PRIMER_SEQUENCE_ID=SSR_00000 Ps_contig_mira-and-tgicl-ass_franssen_26
SEQUENCE=TCTTCTTCGTAGATTTACCTTTKGKATTTTTTTTTTCCTTCCAGTTTCATTATCACCTTCAGAACCTGAATGTAAAGAAGACGACTCATCATTAGATGAGTCATCACCCTCATCATCATCACTCCACGCCCACCAAACTTCTTGGGCTCACAGCGTCTAGGATCAGCAACAAGAAGAGTACGATCATATCGAACAAGAATATCCTTGATCTCCTTCTTACTCTGTTCATCGACATACTTCTGATAGAAAGCCACCAACGCCTTCGCGA
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00001 Ps_contig_mira-and-tgicl-ass_franssen_38
SEQUENCE=AAGCAGTGGTACAACGCAGRGTTACCTTTCTTTTCTTCAACCATAATATCTCAACAACTCTATCCCCCATTCGATTCCTACACTTACTTACTCTCTAATTTTGTTACGAAATATAATTGGTTGCAATTAACATCACTTTGTGATAGCAAAAATGACAGATCAAGAGCAACCAAAAAAGGTAGAACCTGAATCAAGTTCTAATCCTTTATCAGCACCACCACCAAAGGAAGATGTGGCTCAGGAGAAATCTGTAATTCCACAACCTGATCATCTAGATCACAACCCTCCTCCTCCTCCGCCTGTTGTTGATGACTCCAAAGCTCTGGTTGTAGTTCCGAAGACCGAAGAAGCTGCTGAAGAGAAACCACAAGAGGGCTCTATCAACAGAGATGCGGTGCTTGAAAGAGTTGCAACAGAAAAGAGGCTGTCACTGGTCAAAGCATGGGAAGAAAGTGAAAAGTCTAAAGCAGAAAACAAAGCTCAGAGAAAACTGTCTACCATTACAGCATGGGAGAACAGTAAAGAAAGCTGACAACAGAAGCTGAATTAGG
TARGET=284,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00002 Ps_contig_mira-and-tgicl-ass_franssen_99
SEQUENCE=GAATCAGTTTGTCCAAAGAAGGCTTGTTTTCCAAGCAAATAGTCCACCTTAGATAGGAACTCTTCAGGTGAACCATAGTCAGTGATGGACTTCTTATCAGTTGTTTGGACTAAAACCGAAACATTGCTTGTTGCATCAAATTATCCTCATATCTAAGAACCTGACCAGGGAACTCTCTCTCTTTGCTTGGATTCCATTTTGCTGGCACCAGCAGCTTGAATCCATCTCCATTGTATGGCAAGTAATCTGTGTTTGTCTTTGCCTTTCCAAACACATTAGCAGCTTCTCCATAAGCAGCATCTGCAGGTGAAACCTTGGAGCCAACAGCAGCAGCACCAATGAGAACAGAAGTGCTAACCTACGAGACACAACAGCATCAACAACATCATTCTTTGTTTCTGTGCCTTGACAAACAATTRGTTTGGGGTTTTTGGGGTTTTTAGGGTACGGGTTAAAMCTTACGTTTGGGTCGGTTAGGAAGTAAGTTTAAACGGGTTTTCCGTTAAGGGTTTGGGGTAAGGTTTTAGTAAA
TARGET=173,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00003 Ps_contig_mira-and-tgicl-ass_franssen_104
SEQUENCE=AACACATTAGCAGCTTCTCCATAAGTTCAGCTATCTGCAGGTGAAACCTTGGAGCCAACAGCAGCAGCACCAATGAGARCAGAAAGTGCTAACCTACGAGACACAACAGCATCAACAACATCATCTTAGTTTCTGTGCCTTGACAAACAATATTGGTTTGGTTTGGTGGTAACTACTTGGCGTTGAGATAAGGTTCTAGTTGGASTTAGTAATAGCGTATTGGGTGGTGCAAAGAAACATTGTGTAGATGCCTATTTTTTACTTAAAGAGTATGCTATTTAAG
TARGET=153,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00004 Ps_contig_mira-and-tgicl-ass_franssen_123
SEQUENCE=GACAACAACACACAACANGGGTTCTTCATTTTGGACTGTGTGTTTGATTCTAGCATCATTTCTCTCTCTCTGAATTCTGTGCTCTCCCAAGGAAGCCAGTGGATGTACCATTTGGCAGAAACTATTACCCTACTTGGGCTTTTGATCACATCAAATACTTCAATGRCGGTTCTGAGATTCAGCTTCATCTTGATAAATCCACTGGTACTGGTTTTCAGTMCAAAGGTTCATACTTGTTTGGTCATTTCAGTATGAACATTAAGATGGTTCCTGGTGATTCTGCTGGAACAGTTACTGCTTTCTATTTATCTTCACAAAATGCT
TARGET=61,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00005 Ps_contig_mira-and-tgicl-ass_franssen_187
SEQUENCE=GTTAAGTTCTTTGCTGTGTCATGATTTGAAGAACACAGATCTCAAAGTTAGGTTTTATGGGCATTCAGGGTACTTGTTTTTCCCTTTTATTTGAAGGAATTTGTAGTCAGAGTATTAAGGATGCCTATATGCAGTTACAGAAAGTATTCGTTTTATATGGTTTTATGATACCAGAAGACATGCTAATGCTGGTAATGAACTTCATTGTTGATATAGTTTTAGTATTTATTGCAATTTTAGTTCTTGTNTTGCCATTGGACTATCATGATCTCGAAGGGACAAGGTATGTATTTTACCAACCAACCAACCTAAACCCTTTTGTTTAATTTTTTTCCTTACCCTTAACGTTTAAGTAAATTATTTTACCTTAAAAAGTTTAAAAAAAAAAAGT
TARGET=295,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00006 Ps_contig_mira-and-tgicl-ass_franssen_205
SEQUENCE=AAGGCGCAAAGATTTAGACTTTTGAAGACCACCCTCCATATTTGATYCTTTCTTCAACCTATCATCATCATCACCTTAGTCTTCTCCACTTCTCCACCGGACAGTCTCGCCGCCGGAGCCCTTCCCCTTTCTCCGTCTCCTACGACGAATCACCGTTCCCCGATTCAGACTCCCGGCGGCGGGAGCGTGTCCAGATCTATACGAAACCACAGGCCGTAACGAACTACGAGGCGGTCGTTTGGGTCCTTTACGTAAGGAAAAAGTATTAACGTAAGGTTTAAGTAGGAAAAGGTAAGGAAAAAGCG
TARGET=61,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00007 Ps_contig_mira-and-tgicl-ass_franssen_219
SEQUENCE=TATTCGTGTATGGGTGAAGAAAGGGGGAAGTGATCATTTTACCTGCTGGAATTTATCACCGTTTTACATTGGATGAGGGCAATTATATTAAGGCTTTGCGATTTTTTGTTGGTGAACCAGTTTGGACCCCATACAATCGCCCACACGACCATCTCCCTGCAAGAGGGGAATATATCAAGAATTTTGTGGAGAAGGATGTTGCCAACCATACTGTTGACGCCACTGCATAATTTGATTTCTGCTTGATTGTAGCATGGGATAAAAAGGACCGAGGCTTTGTTTGATACTGAAATGGTGTTTGAAAATAATAATAATGGTCTCTTTTGTTTGGTATGTAACCTTTGAGTGTTCGTGTTTGTTACCTGTGTTGTACTAGGATAATTAAAAGTTGCATTTATAAGTATTATTATTATTATTATTATTATTATTATTCAGTATTGGTGACAGAATAAACAGACAGTAATAGGATGTTGATGTATTATATATAATGTTGTACTGTTTTGGTTGCWTTYWACKTAAACT
TARGET=304,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00008 Ps_contig_mira-and-tgicl-ass_franssen_219
SEQUENCE=TATTCGTGTATGGGTGAAGAAAGGGGGAAGTGATCATTTTACCTGCTGGAATTTATCACCGTTTTACATTGGATGAGGGCAATTATATTAAGGCTTTGCGATTTTTTGTTGGTGAACCAGTTTGGACCCCATACAATCGCCCACACGACCATCTCCCTGCAAGAGGGGAATATATCAAGAATTTTGTGGAGAAGGATGTTGCCAACCATACTGTTGACGCCACTGCATAATTTGATTTCTGCTTGATTGTAGCATGGGATAAAAAGGACCGAGGCTTTGTTTGATACTGAAATGGTGTTTGAAAATAATAATAATGGTCTCTTTTGTTTGGTATGTAACCTTTGAGTGTTCGTGTTTGTTACCTGTGTTGTACTAGGATAATTAAAAGTTGCATTTATAAGTATTATTATTATTATTATTATTATTATTATTCAGTATTGGTGACAGAATAAACAGACAGTAATAGGATGTTGATGTATTATATATAATGTTGTACTGTTTTGGTTGCWTTYWACKTAAACT
TARGET=402,30
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00009 Ps_contig_mira-and-tgicl-ass_franssen_304
SEQUENCE=GTTCAGAAATTCATAGATGAACTTGTTAAAATTTGATTTTCCTGGATCAAAAGCAGTATCTGAAGCATCTTCAAAATTTGGAACGGCCTTAGTTTCAGGTCCAATTTTCTTTGTTTTTGAGAAAGTATCAACTTTCATAGTCACAGAAGAAAAAACTGAAGAAGTGGCTAAAACAGAAGAGGAAACAAGCGGTGTAAAAGATAGGGAAATTGTGGTTGAAGAAAGGAAAAAAAGAAGAAACAAGTGAATCTGCAGCTTGTAGAAGGCTGAAGAAAAAGCTAAGGCTGCAAATGACTCTCTGGCTCCGCCGCCGCCGGAGAAAGAGGAAGAGAAACCGGTTGAAACAACTCCGGTGGAAAAAGTGGAACCTCCAAAGGCTTGAAGATGAGACTGTTTTGGAATTGGAGCTATTTGGAGTGAAAATAATTTATATGTAATTATGACTTGTTGTATGTAGGATTGTAATTTGTTATTTGAATGTAATATGAATGATGGTNTAG
TARGET=305,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0822
PRIMER_LEFT_PENALTY=0.048131
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=AAGCTAAGGCTGCAAATGACTC
PRIMER_RIGHT_SEQUENCE=CACTCCAAATAGCTCCAATTCC
PRIMER_LEFT=275,22
PRIMER_RIGHT=418,22
PRIMER_LEFT_TM=60.048
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=0.0822
PRIMER_LEFT_1_PENALTY=0.048131
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=AAGCTAAGGCTGCAAATGACTC
PRIMER_RIGHT_1_SEQUENCE=ACTCCAAATAGCTCCAATTCCA
PRIMER_LEFT_1=275,22
PRIMER_RIGHT_1=417,22
PRIMER_LEFT_1_TM=60.048
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.0914
PRIMER_LEFT_2_PENALTY=0.072552
PRIMER_RIGHT_2_PENALTY=0.018835
PRIMER_LEFT_2_SEQUENCE=AAAACAGAAGAGGAAACAAGCG
PRIMER_RIGHT_2_SEQUENCE=GAGTTGTTTCAACCGGTTTCTC
PRIMER_LEFT_2=169,22
PRIMER_RIGHT_2=349,22
PRIMER_LEFT_2_TM=59.927
PRIMER_RIGHT_2_TM=60.019
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=181
PRIMER_PAIR_PENALTY_3=0.1067
PRIMER_LEFT_3_PENALTY=0.072552
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=AAAACAGAAGAGGAAACAAGCG
PRIMER_RIGHT_3_SEQUENCE=CACTCCAAATAGCTCCAATTCC
PRIMER_LEFT_3=169,22
PRIMER_RIGHT_3=418,22
PRIMER_LEFT_3_TM=59.927
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.1067
PRIMER_LEFT_4_PENALTY=0.072552
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=AAAACAGAAGAGGAAACAAGCG
PRIMER_RIGHT_4_SEQUENCE=ACTCCAAATAGCTCCAATTCCA
PRIMER_LEFT_4=169,22
PRIMER_RIGHT_4=417,22
PRIMER_LEFT_4_TM=59.927
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_00010 Ps_contig_mira-and-tgicl-ass_franssen_309
SEQUENCE=GGCGATTTCCTTTAACGAGGACACACACACGCCACACTCCTCTCAATCTTTTTCCCATTTCCGTTCTGTAGAAAAACCCTAACCATGCTTCTCAAAACCGCCGTAACCGCTTCTTCATCTCCCTCAATTTCTCTCTTCAATTCTGAAAACTCGCTTTTGGTTCCGTTTCTTCACCGAGGAAGTAAACCGGACCGATTAGCGGTTTGTGCTACGAAAGGCTCTAGCAATAACCGTGTTTTAACCGGTGTGTTGTTTGAGCCTTTTGTAGAGGTTAAAAAGGAACTTGATCTTGTTCCTGTTTCTCCACAAGAGTCTCTTGCTCGCCATAAATTTCATGGAGACTCTGAAGCTGCTATTAACGAGCAAATCAACGTGGAATATAATGTTTCGTATGTTTACCATACTATGTATGCGTATTTTGATAGAGATAATGTTGCGCTGAAAGGTCTTGCTAAGTTTTTTAAAGAATCAAGTGAAGAGGAAAGAGGGCATGCTGAGAAGTTGATGGAATATCAGAACAAAAGGGGTGGAAAAGTGAAGTTGCAATCTATAGTGACACCGCTATTGGAACATAAAATGAGAACGCTGTAAATGGTTTATTAAGTGTTAAATTTGGTTATTATATAAGGTTTATGAAGTTTAAACATTAATTTAGGACCATAAACTT
TARGET=21,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.7403
PRIMER_LEFT_PENALTY=2.703497
PRIMER_RIGHT_PENALTY=0.036789
PRIMER_LEFT_SEQUENCE=GCGATTTCCTTTAACGAGGA
PRIMER_RIGHT_SEQUENCE=ACCGGTTAAAACACGGTTATTG
PRIMER_LEFT=1,20
PRIMER_RIGHT=245,22
PRIMER_LEFT_TM=59.297
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=245
PRIMER_PAIR_PENALTY_1=2.8900
PRIMER_LEFT_1_PENALTY=2.703497
PRIMER_RIGHT_1_PENALTY=0.186529
PRIMER_LEFT_1_SEQUENCE=GCGATTTCCTTTAACGAGGA
PRIMER_RIGHT_1_SEQUENCE=CACGGTTATTGCTAGAGCCTTT
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=234,22
PRIMER_LEFT_1_TM=59.297
PRIMER_RIGHT_1_TM=59.813
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=2.9572
PRIMER_LEFT_2_PENALTY=2.920402
PRIMER_RIGHT_2_PENALTY=0.036789
PRIMER_LEFT_2_SEQUENCE=GGCGATTTCCTTTAACGAGG
PRIMER_RIGHT_2_SEQUENCE=ACCGGTTAAAACACGGTTATTG
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=245,22
PRIMER_LEFT_2_TM=60.920
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=2.9859
PRIMER_LEFT_3_PENALTY=2.703497
PRIMER_RIGHT_3_PENALTY=0.282365
PRIMER_LEFT_3_SEQUENCE=GCGATTTCCTTTAACGAGGA
PRIMER_RIGHT_3_SEQUENCE=CGGTTAAAACACGGTTATTGCT
PRIMER_LEFT_3=1,20
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=59.297
PRIMER_RIGHT_3_TM=60.282
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=3.1069
PRIMER_LEFT_4_PENALTY=2.920402
PRIMER_RIGHT_4_PENALTY=0.186529
PRIMER_LEFT_4_SEQUENCE=GGCGATTTCCTTTAACGAGG
PRIMER_RIGHT_4_SEQUENCE=CACGGTTATTGCTAGAGCCTTT
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=234,22
PRIMER_LEFT_4_TM=60.920
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_00011 Ps_contig_mira-and-tgicl-ass_franssen_310
SEQUENCE=ACTTTTTTTAAATTTAGTAGAGAWTCACAATACACATTCACATGCATACTAAACTAAGACAAAGTACWAAATTACTAATTACACAATATAATAAAAGAATAAGAGTATTTTGGTATGTAACATTTTTTTTTGAATATATGCAAACTATTACACATATACAACATATTTATGATCTTCAAGCCTTTGGAGGTTCCACTTTTTCCACCGGAGTTGTTTCAACCGGTTTCTCTTCCTCTTTCTCCGGCGGCGGCGGAGCCAGAGAGTCATTTGCAGCCTTAGTCTTTTTYYTCAGTCCTTCTCAGCTGCAGTATTCTACTTTTGTTTTCTTTTCCTTTTTTCCTCTTACCAACCTACTTAACTTTCCCTATTCCTTTACNCCGTTCTTTCGTTTCCYYTCTKTCTTGWYTACNCCTTACTTTCTTTCTTTAGTTTTTTCTCTGTCTTAGGAACGTACCGACG
TARGET=242,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00012 Ps_contig_mira-and-tgicl-ass_franssen_310
SEQUENCE=ACTTTTTTTAAATTTAGTAGAGAWTCACAATACACATTCACATGCATACTAAACTAAGACAAAGTACWAAATTACTAATTACACAATATAATAAAAGAATAAGAGTATTTTGGTATGTAACATTTTTTTTTGAATATATGCAAACTATTACACATATACAACATATTTATGATCTTCAAGCCTTTGGAGGTTCCACTTTTTCCACCGGAGTTGTTTCAACCGGTTTCTCTTCCTCTTTCTCCGGCGGCGGCGGAGCCAGAGAGTCATTTGCAGCCTTAGTCTTTTTYYTCAGTCCTTCTCAGCTGCAGTATTCTACTTTTGTTTTCTTTTCCTTTTTTCCTCTTACCAACCTACTTAACTTTCCCTATTCCTTTACNCCGTTCTTTCGTTTCCYYTCTKTCTTGWYTACNCCTTACTTTCTTTCTTTAGTTTTTTCTCTGTCTTAGGAACGTACCGACG
TARGET=416,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00013 Ps_contig_mira-and-tgicl-ass_franssen_312
SEQUENCE=GGTTTTTTATTGTAACATTTTTTTGTTAATATATGNAACTATATACATATACAACANATTTATGATCTTCAAGCCCTTTGGTAGGGTTCCACYTTTYYACCGGTTAGTTGTTTCAACCGGTTTCTCTTCCTCTTCTCCGGCGGCGGCGGAGCCAGAGAGTCATTTGCAGCCTTAGCTTTTCTTCAGCCTTCTCAGCTGCAGATTCACTTGTTTCTTCTTTTTTCCTTCTTCAACCACAATTTCCCTATCTTTTACACCGCTTGTTTCCTCTTCTGTTTTAGCCACTTTAACTTAACAAGTTTTTTACTTCTGTGACTATGAAAGTTNATACTTTCACAAAAACAAAGAAATTGGACCTGAAACTAAGGCCGTTACC
TARGET=138,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00014 Ps_contig_mira-and-tgicl-ass_franssen_344
SEQUENCE=GGTTGACGGAAGCTTCCACCATAACCCGATGAGTTACTACCTAGATCGACGATGGTATTCTTGGCCAGTGACCTTCTCCATGATTGTAGGGTGCTGATCAACCATGGTGACTTTCCTTTCACGTAGGGTTTTAGCGAAAGGATGAGAGAACGCTAGAGAGAGAGACGCAGGAAGAGGTTTGTGTGGAGGCGTGTGCGTGCCTTTTC
TARGET=155,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2568
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=1.252475
PRIMER_LEFT_SEQUENCE=GTATTCTTGGCCAGTGACCTTC
PRIMER_RIGHT_SEQUENCE=CTCCACACAAACCTCTTCCTG
PRIMER_LEFT=54,22
PRIMER_RIGHT=187,21
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.748
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=1.3828
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=1.252475
PRIMER_LEFT_1_SEQUENCE=TATTCTTGGCCAGTGACCTTCT
PRIMER_RIGHT_1_SEQUENCE=CTCCACACAAACCTCTTCCTG
PRIMER_LEFT_1=55,22
PRIMER_RIGHT_1=187,21
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.748
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=1.4258
PRIMER_LEFT_2_PENALTY=0.173354
PRIMER_RIGHT_2_PENALTY=1.252475
PRIMER_LEFT_2_SEQUENCE=GACCTTCTCCATGATTGTAGGG
PRIMER_RIGHT_2_SEQUENCE=CTCCACACAAACCTCTTCCTG
PRIMER_LEFT_2=69,22
PRIMER_RIGHT_2=187,21
PRIMER_LEFT_2_TM=59.827
PRIMER_RIGHT_2_TM=59.748
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=119
PRIMER_PAIR_PENALTY_3=1.5330
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=1.528639
PRIMER_LEFT_3_SEQUENCE=GTATTCTTGGCCAGTGACCTTC
PRIMER_RIGHT_3_SEQUENCE=CCTCCACACAAACCTCTTCCT
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=188,21
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=60.529
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=1.5925
PRIMER_LEFT_4_PENALTY=0.340007
PRIMER_RIGHT_4_PENALTY=1.252475
PRIMER_LEFT_4_SEQUENCE=AGATCGACGATGGTATTCTTGG
PRIMER_RIGHT_4_SEQUENCE=CTCCACACAAACCTCTTCCTG
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=187,21
PRIMER_LEFT_4_TM=60.340
PRIMER_RIGHT_4_TM=59.748
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_00015 Ps_contig_mira-and-tgicl-ass_franssen_367
SEQUENCE=TTTGAGTCGTTTGGGGGTACGTTGTAAGTTTGGTCCTTTCGTCCACTTTCMTCCGTATGAACATACAACCATATATCTAATTGACCACATCTTGATTACTAAGTATTCTTCTCCCTAAAATGTACTATATAATTACCCTTCTTAATTAATTATGAGCTAAATTTCAAAATAAATAAGTAATAGATGGAAGTAATTATTCTCCATCACACATTCTCTCATATAAAGCTCCAAAACAAAATAATGTTACTTTTGTTGCATAAATAAATAAACAAGTTAATATCAAAGTGTGTAATTTATGGTGTTTCACTTTCACCACTAAATTTTTCTATGTTTGTTAGGTCAAAGATGTGGCCACGATTCTACATTGGAAGCTATAATCTCGTGGTTAAAGG
TARGET=257,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00016 Ps_contig_mira-and-tgicl-ass_franssen_378
SEQUENCE=TTTTACGTTTTCGGGGTTTTTAAGTTTHGVGACATCAAACATTACTATATAGCATTGTCATTTGAATCTTCATTGCACCAATGTCTACCTCTTCAATAAACGGCTTTAGTCTTTCTTCTTTGTCCCCTGCCAAACTTCTACCAAAAGAACTACATTGAGACCCTTTGTTTCTGCATCTCTTAACACTTCTTCTTCATCTTCTTCCTCGACTTCCTTCTCTTATCAGACAGCCGGTTTTCGTCTTCTTCTTCTCCTATCATCA
TARGET=241,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00017 Ps_contig_mira-and-tgicl-ass_franssen_392
SEQUENCE=TRKTGGACCTTGTAATTAGGAGCTCTATTTAATCATGGCTTAAGTATGACAAATCATTATGCAACACATTCATGAAGTAAAGGCTTAGAATATCTTGTATACGAATGTTACTCATGTTTAAAGACAATAAATATCCGCCATAGGAAAGGTTACATTCTAAACAAATACCAGAACTCAGAAACTGTTCTATCTATCTATCACCTTCAGCTGAATATTTCGTAAAATTTACTCGGTGACGAGTAAACCCGAGTCTATCGACTCCAAAGGATCCTTTCTAAGACGGTGTCTCAAGCAGTTAGGGATGACAGTAGCAATATCCTCTACACTCACTTTGTCTCTTCCCTTCAAAGCAGCCAAAGCTTTAGCAGCTCTATTTGATACAATGTCTCCTCTCAATCCGTCCACGTTCAACTCTGCGTTGATACCACTGCTT
TARGET=186,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00018 Ps_contig_mira-and-tgicl-ass_franssen_394
SEQUENCE=TGTAACGTTGGTACGGATTGAGAGGAGACATTGTATCAAATAGAGCTGCTAAAGCTTTGGCTGCTTTGAAGGGAAGAGACAAAGTGAGTGTAGAGGATATTGCTACTGTCATCCCTAACTGCTTGAGACCACCGTCTTAAGAAAGGATTCCTTTGGAGTCGATAGACTTCGGGTTTACTCGTCSAACCGAGTAAATTTTACGAATATTCAGCTGAAGGTGATAGATAGATAGAM
TARGET=220,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00019 Ps_contig_mira-and-tgicl-ass_franssen_395
SEQUENCE=TGAACGTGGACGGATTGAGAGGAGACATTGTATCAAATAGAGCTGCTAAAGCTTTGGCTACTTTGAAGGGAAGAGACAAAGTGAGTGTAGAGGATATTGCTACTGTCRTCCCTAACTGCTTGAGACACCGTCTTAGAAAGGATCCTTTGGAGTCGATAGACTTCGGGGTTTTACTCGTCACCGAAGTAAATTTTACGAAATATTCAGCTGAAGGTGATAGATAGATAGAACWGTTT
TARGET=216,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00020 Ps_contig_mira-and-tgicl-ass_franssen_480
SEQUENCE=GTTCGGAGGRAAAAAATTAAACSGAGGAAGAAAGAGAGGGCTTCGACTCAACGCACTTTCTCTCTTCCTCTTCTTCTTCTTCCCACCCAAGTCATGAGCCTCTTCGGTATTGGCAGAAATCAGAGAACATTCCGACCCAAAAAAAAGTCTCCTTCTGGAAGCAAGGGAGCTCAGCTTAAGAAAACACATTGATGCAACATTAGGTAGTGGAAATCTGGAAGGGAAGCAAGTAAAGCTACCTCCGTGGAGAGGTATTTGAAT
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00021 Ps_contig_mira-and-tgicl-ass_franssen_649
SEQUENCE=TACGTCGGGGACCTTCCCTCTTCTCAACTCATCTTTTCCTTTTACTGAAAATCTGAACTAAATCTCATCACAATGGACGCAAGCAAACAAACAAAGAAAGGAGCCGGAGGAAGGAAAGGAGGAGGACCAAGGAAGAAGGCGGTCACAAGATCCGTCAGAGCCGGACTTCAATTTCCAGTTGGAAGAATCGGTCGATTCTTGAAGAAAGGTAGATACGCTCAGCGTGTTGGTACCGGTGCTCCTGTTTACTTGGCCGCTGTTCTTGAATATCTTGCTGCTGAGGTTCTTGAGTTGGCTGGAAATGCAGCACGTGATAACAAGAAGAAAAGTATCAGTCCAAGGCATTTGTTGTTGGCTGTGAGGAACGATGTAGAACTTGGAAAATTGCTTGCTGGTGTTACCATTGCTCACGGTGGTGTTCTTCCTAACATCAACCCAGTGTTATTGCCCAAGAGAAATGAAAACGCTGCCAGTACTCCCAAGTCTCCATCAAAGGCCAAGAAATCTCCAAAGAAGGCTTAGATAGATGACTTTAGATGTTAGGTTTTGTTTCTTCCTTTTTGTTGTGATGATATGTAAAACCCCTATGATGGAGCAGGGATATATTAACTTATACCAACCGATGTAATTCAATAGTCTCTGAATTTTCTGACTAGTATTATTCATCAATGAAAACGATTTGTTTTCTGTHMAC
TARGET=84,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00022 Ps_contig_mira-and-tgicl-ass_franssen_676
SEQUENCE=CGATAGAAAACGAAGCACATGAACACCAAATGCATAAAACAAGTGAACACCAAATATAGTCAATTCCACTATTCATAGAATACACAAAAGATCCAACGGATCACATTACATCACCTTACAAGTTAAACATGAATCCTCTAAACAACGATACTGTTACAACTCTAAGCGCTAGTGAACTTGGTTACAGCTTTGGTACCCTCAGATACAGCATGCTTGGCCAATTCTCCAGGAAGAACAAGCCTAACCGCAGTCTGAATTTCCCTCGAAGTTATCGTTGGCTTCTTGTTATACCTCGCAAGCCTCGAAGATTCAGCAGCAAGCTTCTCAAAGATATCATTGATGAAACTGTTCATTATACCCATGGCCTTGCTTGAGATACCAATGTCAGGGTGAACTTGTTTCAGAACCTTGAAGATGTAGATCTTGTAGGTTTCCACATTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCAGCGGCGCCACCGTCCTTGGGTAGCTTCTTTCCGGCCTTTGGCTTCTTCTCGGCGGGAGTCTTCTCGGCTATTGTGGATTTCTTCTCCTCTACAGGTTTCTTCTCAGCTGGTTTCTTCTCTCCTTTGGTGCCATTTTTGTTTGGGAAATGGAAATTGGAAATTGAAAGAAAATAGAAAGA
TARGET=452,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1735
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.078029
PRIMER_LEFT_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_SEQUENCE=AAACCAGCTGAGAAGAAACCTG
PRIMER_LEFT=345,22
PRIMER_RIGHT=586,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.922
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.1909
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_1_SEQUENCE=AAATCCACAATAGCCGAGAAGA
PRIMER_LEFT_1=345,22
PRIMER_RIGHT_1=553,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.1909
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.095445
PRIMER_LEFT_2_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_2_SEQUENCE=AGAAATCCACAATAGCCGAGAA
PRIMER_LEFT_2=345,22
PRIMER_RIGHT_2=555,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.1909
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.095445
PRIMER_LEFT_3_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_3_SEQUENCE=AAGAAATCCACAATAGCCGAGA
PRIMER_LEFT_3=345,22
PRIMER_RIGHT_3=556,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=60.095
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.1909
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=0.095445
PRIMER_LEFT_4_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_4_SEQUENCE=GAAATCCACAATAGCCGAGAAG
PRIMER_LEFT_4=345,22
PRIMER_RIGHT_4=554,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=60.095
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_00023 Ps_contig_mira-and-tgicl-ass_franssen_680
SEQUENCE=ACGGTGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAACAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGTATCATGAACAGTTTCATCAACGACATTTTCGAGAAGCTTGCTGCTGAGTCTTCAAGACTTGCAAGGTACAACAAGAAGCCAACAATCACTTCAAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGAGAATTGGCCAAGCATGCCGTCTCTGAGGGTACCAAGGCTGTGACTAAGTTCACAAGCTCTTAAACTCAATATCTAGGTTTTTTCTGGGTCGCTATGTAGGCACCGTTATACCTTTCTAGTGTTGTAATCTGCTAATGGGATCGCGCTGATCCTTTTATTGTGTATGAACTTATCTTATTATTCTTAACATGATTAATGGTATTGGGCTTCAAAAATTGTTTTTATTGTAACACC
TARGET=24,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00024 Ps_contig_mira-and-tgicl-ass_franssen_681
SEQUENCE=CCTAACAGGGAGGTACCATATCACATAATTCAAGAACTGGTGAATTTGGTGACGGCCTTTGTACCCTCGGAAACGGCGTGTTTGGCCAATTCACCAGGAAGTACCAATCTAACAGCGGTTTGAATCTCCCTTGAAGTAATAGTTGGCTTCTTGTTATACCTAGCCAAACGAGAGGATTCTTGAGCTAGTTTCTCGAAGATGTCGTTGATGAAACTATTCATGATTCCCATAGCCTTGCTGGAGACTCCGATATCAGGATGAACCTGTTTCAAAACCTTGAAGATGTAGATCTTGTAGGTCTCAACGCTCTTCTTGGATTTCTTCTTCTTCTTGTCGCTTCCTCCTTCCTTCGAGATCTTCTTCTCAGCCTTAGGTTTCTTCTCTGCAGGGGATTTCTCTGCTGCGGGTTTCTTCTCGGCTGGCTTCTTCTCGGCCTTTGTTGGAGCCATTGGTGATGTTGGTTGAATTGATGGGTTGGTCGTAGGAAGAAAAGAGAGGTTGATACAGAGATGGAGTGTTGAATCCCCGCGTACTCTGACGTTAG
TARGET=319,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2040
PRIMER_LEFT_PENALTY=0.108206
PRIMER_RIGHT_PENALTY=0.095771
PRIMER_LEFT_SEQUENCE=GTCTCAACGCTCTTCTTGGATT
PRIMER_RIGHT_SEQUENCE=CCATCAATTCAACCAACATCAC
PRIMER_LEFT=297,22
PRIMER_RIGHT=472,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.2040
PRIMER_LEFT_1_PENALTY=0.108206
PRIMER_RIGHT_1_PENALTY=0.095771
PRIMER_LEFT_1_SEQUENCE=GTCTCAACGCTCTTCTTGGATT
PRIMER_RIGHT_1_SEQUENCE=CATCAATTCAACCAACATCACC
PRIMER_LEFT_1=297,22
PRIMER_RIGHT_1=471,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=175
PRIMER_PAIR_PENALTY_2=0.2136
PRIMER_LEFT_2_PENALTY=0.117817
PRIMER_RIGHT_2_PENALTY=0.095771
PRIMER_LEFT_2_SEQUENCE=TCGAAGATGTCGTTGATGAAAC
PRIMER_RIGHT_2_SEQUENCE=CCATCAATTCAACCAACATCAC
PRIMER_LEFT_2=192,22
PRIMER_RIGHT_2=472,22
PRIMER_LEFT_2_TM=60.118
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=281
PRIMER_PAIR_PENALTY_3=0.2136
PRIMER_LEFT_3_PENALTY=0.117817
PRIMER_RIGHT_3_PENALTY=0.095771
PRIMER_LEFT_3_SEQUENCE=TCGAAGATGTCGTTGATGAAAC
PRIMER_RIGHT_3_SEQUENCE=CATCAATTCAACCAACATCACC
PRIMER_LEFT_3=192,22
PRIMER_RIGHT_3=471,22
PRIMER_LEFT_3_TM=60.118
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.2665
PRIMER_LEFT_4_PENALTY=0.170703
PRIMER_RIGHT_4_PENALTY=0.095771
PRIMER_LEFT_4_SEQUENCE=ATTCATGATTCCCATAGCCTTG
PRIMER_RIGHT_4_SEQUENCE=CATCAATTCAACCAACATCACC
PRIMER_LEFT_4=215,22
PRIMER_RIGHT_4=471,22
PRIMER_LEFT_4_TM=60.171
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_00025 Ps_contig_mira-and-tgicl-ass_franssen_683
SEQUENCE=AGGTTTCAAGAAAAGATTCATATTATCATTAATATTCAAAAACACATTTGTAAATCTACAATCCTTTTTATAAAATTTAAAACAAAAGACCAATTTATGATAAACTAAACCCTAATTCAAAGAAAAAGGGAAAAACAGAAAACAAATCAAGAACTGGTGAATTTTGTAACGGCCTTGGTACCCTCTGAGACGGCATGTTTAGCCAATTCACCGGGAAGAACCAATCTAACAGCGGTTTGAATTTCCCGAGAAGTAATCGTTGGCTTCTTGTTGTACCTAGCCAACCGAGACGATTCTTGAGCGAGTTTCTCAAAGATATCGTTGATGAAACTGTTCATGATTCCCATGGCCTTGCTTGAGACACCAATATCGGGGTGAACCTGTTTCAAAACCTTGAAGATGTAAATCTTGTAGGTTTCAATGCTCTTCTTGGTTCTCTTCTTCTTCTTGTCGCTTCCTCCTTCTTTTGAGATCTTCTTCTCGGCCTTTGGTTTCTTCTCGGCGGGGGATTTTTCTACGGCGGGCTTCTTCTCCGCTGGTTTCTTCTCGGCCTTTGTTGGAGCCATTGATGGTGTTGATTGGTAGGAAATTGGGTTGGTTGTGAATGCGAAAGAGATGAAGTGATGAATCCCCGABTA
TARGET=437,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00026 Ps_contig_mira-and-tgicl-ass_franssen_684
SEQUENCE=TCAACGCAGAGTAYTCGGGGACCACAATCTCAATTCTTCATCTAATTTCATCTCAACATTCCAATCTCAAACCCTATTTTCCCCAATGGCGCCAAAAGGAGAGAAGAAGCCAGCCGAGAAGAAACCCGTAGAGGAGAAGAAATCCACGGTAGCCGAGAAGGTTCCGGCTGAGAAGAAGCCAAAGGCCGGCAAGAAGCTGCCTAAAGACGGTGGTGCCACCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGAATCATGAACAGTTTCATCAATGATATTTTCGAGAAGCTTGCTGCGGAGTCGTCTAGACTTGCGAGGTACAACAAGAAGCCGACGATAACTTCGAGGGAAATTCAGACCGCGGTCAGGCTCGTTCTGCCCGGTGAATTGGCTAAGCATGCTGTTTCTGAGGGTACCAAAGCTGTAACTAAGTTCACTAGTTCTTAGATTTGAAACTGTTTTTAGGGGTTTTTGTATGCTTAAGTAGATCTAATGGGTCTGAATATTTTCTTTGAAACTGTGGATTTTATTTTATGACTCATAAAGTAATATGATTCAGTGAATTGACTCAAAGGTTATCTGTAAATGTTTTCTCTTTGAACAAAATCAAACAAGAAAACTAATTAAA
TARGET=230,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00027 Ps_contig_mira-and-tgicl-ass_franssen_689
SEQUENCE=CTCCAACGAAGGCCGAGAAGAAGCCAGCCGAGAAGAAACCCGCAGCAGAGAAGTCCCCTGCAGAGAWGAAACCTAAGGCTGAGAAGAAGATCTCGAAGGAAGGAGGAAGCGACAAGAAGAAGAAGAAGTCCAAGAAGAGCGTTGAGACCTACAAGATCTACATCTTCAAGGTGTTGAAGCAGGTTCATCCTGATATCGGTGTCTCCAGCAAGGCTATGGGAATCATGAACAGTTTCATCAACGACATTTTTGAGAAACTCGCTCAGGAATCCTCCCGTTTGGCTAGGTATAACAAGAAGCCAACTATTACATCTCGGGAGATTCAAACCGCTGTTAGATTGGTTCTCCCCGGGGAGTTGGCCAAACACGCCGTCTCCGAGGGTACCAAGGCCGTCACCAAATTCACCAGTTCTTGAATTATCTGCTCTGTTATTATTCTCTCTATAGGGTTTAGCTTGATTTTTGTTGAATTAGTTTTTTTTAATGGAGCATTGATGTATATAATGGTTATAATCAATTTAAATGATATTTAAAATTCTTCACTTTWTAAT
TARGET=114,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00028 Ps_contig_mira-and-tgicl-ass_franssen_690
SEQUENCE=AAGCTCCGGTCGAGAAGAAGCCAAAGGCCGGAAAGAAGCTGCCTAAGGATGGCGGCGCCGCTGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGTATCATGAACAGTTTCATCAACGACATCTTCGAGAAGCTTGCTGCTGAATCTTCAAGACTTGCAAGATACAACAAGAAGCCAACAATCACTTCAAGGGAAATTCAGACTGCAGTTAGACTTGTTCTCCCTGGAGAATTGGCCAAGCATGCTGTCTCTGAAGGAACAAAAGCCGTGACCAAGTTTACCAGTGCTTAAACTGTACCATTTGTAGTGTTGTTTCAGAAGTGGTTCTTGTTAATGTTTATCTCTCTTGGTATCAATTAAATCCGAATTATTCTGTAATTTTCTTTTAAATATTAAAATAATATCTTGTCTATAGAATAATTATATGTYYTTTGTCGTCTTGTAGGGCACAGTTTATAATGCGACA
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00029 Ps_contig_mira-and-tgicl-ass_franssen_691
SEQUENCE=CKYGGGGAACCACATCCATATTCAATTTGCAAATTCGAAAACCTAAATTCTCAATTTCTTCAATGGCGCCAAAGGGGGAGAAGAAGCCAGCTGAGAAGAAACCCGCGGAGGAGAAGAAAACGACTGTAGCTGAGAAGGCTCCGGCTGAGAAGAAGCCAAAGGCCGGAAAGAAGCTGCCTAAGGATGGCGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGTGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAACAAGTTCACCCTGACATTGGCATCTCAAGCAAGGCCATGGGTATCATGAACAGTTTCATCAACGACATCTTCGAGAAGCTAGCTGCCGAATCTTCAAGACTTGCAAGGTACAACAAGAAGCCAACAATCACTTCTAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGAGAATTGGCCAAGCATGCCGTCTCTGAGGGTACCAAAGCTGTGACTAAGTTCACTAGTTCTTAGATTTTTAGGTTCTTGTTTGCTGCGTTGATTTTGAACTTTTATCTTTTGTAAATTGGAATGAAGTAGTGGGAACCGTTGAATCTTTTCTGTGTATGAAAATGATGATTTTATTCTAATATACTTCAAAATGACTTATATAAAACTTGCTTTAAAATTAAAAAAGGTTT
TARGET=207,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00030 Ps_contig_mira-and-tgicl-ass_franssen_692
SEQUENCE=GTTCGAACACCAAATATAATCAATTCCACCATTTTCAATACACAAAGGATCCAATATATCCCATTACTTCATCATACAAGCTAAAGAGTAGAAAAACTACTCTTGAAATCTATAATAACWTAAAACCAGATCCAGTAAACAAGAACCCATTAAACAACAACAAAACTGTTACAACTCTAACCGCTTGTAAACTTGGTTACAGCTTTAGTACCCTCAGACACGGCGTGTTTGGCCAATTCTCCGGGAAGAACAAGCCTAACCGCAGTCTGAATCTCCCTCGAAGTTATCGTCGGCTTCTTGTTATACCTCGCAAGCCTCGAAGATTCAGCAGCAAGCTTCTCAAAGATGTCGTTGATGAAACTGTTCATTATACCCATGGCCTTGCTTGAGATACCAATGTCAGGGTGAACTTGTTTCAGAACCTTGAAGATGTAGATCTTGTAGGTCTCCACACTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCGGCGGCGCCACCGTCCTTGGGTAGTTTCTTTCCGGCCTTGGGTTTCTTCTCTACTGAATTTGTATC
TARGET=467,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00031 Ps_contig_mira-and-tgicl-ass_franssen_693
SEQUENCE=CTTTGGTACCCTCAGATACAGCATGCTTGGCCAATTCTCCAGGAAGAACGAGCCTAACCGCAGTCTGAATCTCCCTCGAAGTTATCGTTGGCTTCTTGTTATACCTCGCAAGTCTCGAAGATTCAGCAGCAAGCTTCTCAAAGATATCGTTGATGAAACTGTTCATTATACCCATGGCCTTGCTTGAGATACCAATGTCAGGGTGAACTTGTTTCAGAACCTTGAAGATGTAGATCTTGTAGGTTTCCACATTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCNGCGGCGCCACCGTCCGTTGGGTACGTCCTTTCCGTTTCCCGGTCCTTTGGTCTTTCCTTCGTCGGGCGGGTAGTTCCTTCGTCGGTCTTATTGGTGGTTACTTTTCCTTCCTTCCCTTCCTTAACGTTACGGTTTTCCTTCCTCGTA
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0969
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.001455
PRIMER_LEFT_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_SEQUENCE=AACGTTAAGGAAGGGAAGGAAG
PRIMER_LEFT=158,22
PRIMER_RIGHT=414,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.999
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.1488
PRIMER_LEFT_1_PENALTY=0.147295
PRIMER_RIGHT_1_PENALTY=0.001455
PRIMER_LEFT_1_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_RIGHT_1_SEQUENCE=AACGTTAAGGAAGGGAAGGAAG
PRIMER_LEFT_1=183,22
PRIMER_RIGHT_1=414,22
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=59.999
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.1499
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.054491
PRIMER_LEFT_2_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_2_SEQUENCE=GGAAGGGAAGGAAGGAAAAGTA
PRIMER_LEFT_2=158,22
PRIMER_RIGHT_2=406,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=59.946
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.1499
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.054491
PRIMER_LEFT_3_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_3_SEQUENCE=GTTAAGGAAGGGAAGGAAGGAA
PRIMER_LEFT_3=158,22
PRIMER_RIGHT_3=411,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=59.946
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.1867
PRIMER_LEFT_4_PENALTY=0.185267
PRIMER_RIGHT_4_PENALTY=0.001455
PRIMER_LEFT_4_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_4_SEQUENCE=AACGTTAAGGAAGGGAAGGAAG
PRIMER_LEFT_4=195,22
PRIMER_RIGHT_4=414,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=59.999
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_00032 Ps_contig_mira-and-tgicl-ass_franssen_693
SEQUENCE=CTTTGGTACCCTCAGATACAGCATGCTTGGCCAATTCTCCAGGAAGAACGAGCCTAACCGCAGTCTGAATCTCCCTCGAAGTTATCGTTGGCTTCTTGTTATACCTCGCAAGTCTCGAAGATTCAGCAGCAAGCTTCTCAAAGATATCGTTGATGAAACTGTTCATTATACCCATGGCCTTGCTTGAGATACCAATGTCAGGGTGAACTTGTTTCAGAACCTTGAAGATGTAGATCTTGTAGGTTTCCACATTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCNGCGGCGCCACCGTCCGTTGGGTACGTCCTTTCCGTTTCCCGGTCCTTTGGTCTTTCCTTCGTCGGGCGGGTAGTTCCTTCGTCGGTCTTATTGGTGGTTACTTTTCCTTCCTTCCCTTCCTTAACGTTACGGTTTTCCTTCCTCGTA
TARGET=391,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5560
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.460533
PRIMER_LEFT_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_SEQUENCE=TACGAGGAAGGAAAACCGTAAC
PRIMER_LEFT=158,22
PRIMER_RIGHT=433,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.539
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.6078
PRIMER_LEFT_1_PENALTY=0.147295
PRIMER_RIGHT_1_PENALTY=0.460533
PRIMER_LEFT_1_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_RIGHT_1_SEQUENCE=TACGAGGAAGGAAAACCGTAAC
PRIMER_LEFT_1=183,22
PRIMER_RIGHT_1=433,22
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=59.539
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=0.6458
PRIMER_LEFT_2_PENALTY=0.185267
PRIMER_RIGHT_2_PENALTY=0.460533
PRIMER_LEFT_2_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_2_SEQUENCE=TACGAGGAAGGAAAACCGTAAC
PRIMER_LEFT_2=195,22
PRIMER_RIGHT_2=433,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=59.539
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.8356
PRIMER_LEFT_3_PENALTY=0.375090
PRIMER_RIGHT_3_PENALTY=0.460533
PRIMER_LEFT_3_SEQUENCE=TGAGATACCAATGTCAGGGTGA
PRIMER_RIGHT_3_SEQUENCE=TACGAGGAAGGAAAACCGTAAC
PRIMER_LEFT_3=184,22
PRIMER_RIGHT_3=433,22
PRIMER_LEFT_3_TM=60.375
PRIMER_RIGHT_3_TM=59.539
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.9360
PRIMER_LEFT_4_PENALTY=0.475463
PRIMER_RIGHT_4_PENALTY=0.460533
PRIMER_LEFT_4_SEQUENCE=GGGTGAACTTGTTTCAGAACCT
PRIMER_RIGHT_4_SEQUENCE=TACGAGGAAGGAAAACCGTAAC
PRIMER_LEFT_4=200,22
PRIMER_RIGHT_4=433,22
PRIMER_LEFT_4_TM=59.525
PRIMER_RIGHT_4_TM=59.539
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_00033 Ps_contig_mira-and-tgicl-ass_franssen_694
SEQUENCE=CGGAGAAGAAACCGTGGAGGAGAAGAAGTCCACCATAGCAGAGAAGGCTCCTGCCGAGAAGAAGCCGAAGGCCGGAAAGAAGCTGCCTAAGGACGGTGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAACAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGTATCATGAACAGTTTCATCAACGACATTTTCGAGAAGCTTAGCTGCTGAGTCTTCAAGACTTGCAAGGTACAACAAGAAGCCAACAATCACTTCAAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGAGAATTGGCCAAGCATGCCGTCTCTGAGGGTACCAAGGCTGTGACTAAGTTCACAGCTTACTTAAAACTCAAATTTACG
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3743
PRIMER_LEFT_PENALTY=0.226971
PRIMER_RIGHT_PENALTY=0.147295
PRIMER_LEFT_SEQUENCE=AAGTCCACCATAGCAGAGAAGG
PRIMER_RIGHT_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT=25,22
PRIMER_RIGHT=208,22
PRIMER_LEFT_TM=59.773
PRIMER_RIGHT_TM=59.853
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.4122
PRIMER_LEFT_1_PENALTY=0.226971
PRIMER_RIGHT_1_PENALTY=0.185267
PRIMER_LEFT_1_SEQUENCE=AAGTCCACCATAGCAGAGAAGG
PRIMER_RIGHT_1_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=196,22
PRIMER_LEFT_1_TM=59.773
PRIMER_RIGHT_1_TM=60.185
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.4805
PRIMER_LEFT_2_PENALTY=0.226971
PRIMER_RIGHT_2_PENALTY=0.253482
PRIMER_LEFT_2_SEQUENCE=AAGTCCACCATAGCAGAGAAGG
PRIMER_RIGHT_2_SEQUENCE=CTTCTCGAAAATGTCGTTGATG
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=258,22
PRIMER_LEFT_2_TM=59.773
PRIMER_RIGHT_2_TM=59.747
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=234
PRIMER_PAIR_PENALTY_3=0.5712
PRIMER_LEFT_3_PENALTY=0.423908
PRIMER_RIGHT_3_PENALTY=0.147295
PRIMER_LEFT_3_SEQUENCE=GGAGGAGAAGAAGTCCACCATA
PRIMER_RIGHT_3_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_3=15,22
PRIMER_RIGHT_3=208,22
PRIMER_LEFT_3_TM=59.576
PRIMER_RIGHT_3_TM=59.853
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=0.6021
PRIMER_LEFT_4_PENALTY=0.226971
PRIMER_RIGHT_4_PENALTY=0.375090
PRIMER_LEFT_4_SEQUENCE=AAGTCCACCATAGCAGAGAAGG
PRIMER_RIGHT_4_SEQUENCE=TGAGATACCAATGTCAGGGTGA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=207,22
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=60.375
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_00034 Ps_contig_mira-and-tgicl-ass_franssen_695
SEQUENCE=ATGGCCTTGCTTGAGATGCCAATGTCAGGGTGAACTTGTTTCAGAACTCTGAAGATGTAGATCTTGTATGTCTCCACACTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCGGCGGCACCGCCATCCTTAGGCAGCTTCTTTCCGGCCTTTGGCTTCTTCTCAGCCGGAGCCTTCTCAGCTACGGTCGTTTTCTTCTCCTCCGCGGGTTTCTTCTCAGCTGGCTTCTTCTCCCCTTGGCGCCATTGAAGAAATTAG
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6097
PRIMER_LEFT_PENALTY=0.185267
PRIMER_RIGHT_PENALTY=0.424392
PRIMER_LEFT_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_SEQUENCE=GAAAACGACCGTAGCTGAGAAG
PRIMER_LEFT=22,22
PRIMER_RIGHT=194,22
PRIMER_LEFT_TM=60.185
PRIMER_RIGHT_TM=60.424
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.6097
PRIMER_LEFT_1_PENALTY=0.185267
PRIMER_RIGHT_1_PENALTY=0.424392
PRIMER_LEFT_1_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_1_SEQUENCE=GAGAAGAAAACGACCGTAGCTG
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=199,22
PRIMER_LEFT_1_TM=60.185
PRIMER_RIGHT_1_TM=60.424
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.6097
PRIMER_LEFT_2_PENALTY=0.185267
PRIMER_RIGHT_2_PENALTY=0.424392
PRIMER_LEFT_2_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_2_SEQUENCE=GAAGAAAACGACCGTAGCTGAG
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=60.424
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=176
PRIMER_PAIR_PENALTY_3=0.7743
PRIMER_LEFT_3_PENALTY=0.185267
PRIMER_RIGHT_3_PENALTY=0.589035
PRIMER_LEFT_3_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_3_SEQUENCE=GAGGAGAAGAAAACGACCGTAG
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=202,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=59.411
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.8810
PRIMER_LEFT_4_PENALTY=0.185267
PRIMER_RIGHT_4_PENALTY=0.695721
PRIMER_LEFT_4_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_4_SEQUENCE=AAGAAGCCAGCTGAGAAGAAAC
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=229,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=59.304
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_00035 Ps_contig_mira-and-tgicl-ass_franssen_697
SEQUENCE=AAGTCTAACCGCGGTCTGAATTTCCCTTGATGTTAATTGTTGGCTTCTTGTTGTATCCTGCAAGTCTTGAAGACTCTGCAGCAAGCTTCTCGAAGATGTCGTTGATGAAACTATTCATGATACCCATGGCCTTGCTTGAGATACCAATGTCGGGGTGAACTTGCTTCAGAACCTTGAAGATGTAGATCTTGTATGTCTCCACACTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCAGCGGCACCGCCATCCTTAGGCAGCTTCTTTCCGGCCTTTGGTTTCTTCTCGGCAGGAGCCTTCTCGGCCACCGTAGACTTCTTCTCTTCGACGGGTTTCTTCTCAGCAGGCTTCTTCTCTCCCTTTGGCGCCATTACAAGAATTGAGAAATTTAGGGTTTCGAATTGGAAATTGGATATTAAAACTGAAACGAGATC
TARGET=217,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0424
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TGAACTTGCTTCAGAACCTTGA
PRIMER_RIGHT_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_LEFT=155,22
PRIMER_RIGHT=333,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.0734
PRIMER_LEFT_1_PENALTY=0.061230
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=CTATTCATGATACCCATGGCCT
PRIMER_RIGHT_1_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_LEFT_1=110,22
PRIMER_RIGHT_1=333,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.1233
PRIMER_LEFT_2_PENALTY=0.030276
PRIMER_RIGHT_2_PENALTY=0.093026
PRIMER_LEFT_2_SEQUENCE=TGAACTTGCTTCAGAACCTTGA
PRIMER_RIGHT_2_SEQUENCE=AGAAGAAGCCTGCTGAGAAGAA
PRIMER_LEFT_2=155,22
PRIMER_RIGHT_2=356,22
PRIMER_LEFT_2_TM=60.030
PRIMER_RIGHT_2_TM=59.907
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.1300
PRIMER_LEFT_3_PENALTY=0.117817
PRIMER_RIGHT_3_PENALTY=0.012153
PRIMER_LEFT_3_SEQUENCE=TCGAAGATGTCGTTGATGAAAC
PRIMER_RIGHT_3_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_LEFT_3=89,22
PRIMER_RIGHT_3=333,22
PRIMER_LEFT_3_TM=60.118
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.1368
PRIMER_LEFT_4_PENALTY=0.124655
PRIMER_RIGHT_4_PENALTY=0.012153
PRIMER_LEFT_4_SEQUENCE=CTTCTCGAAGATGTCGTTGATG
PRIMER_RIGHT_4_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_LEFT_4=85,22
PRIMER_RIGHT_4=333,22
PRIMER_LEFT_4_TM=59.875
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_00036 Ps_contig_mira-and-tgicl-ass_franssen_698
SEQUENCE=GTACGCGGGGACACCCTAATATCTGATTACGAACCCTATTTTTTCTCGAAATTTCAAATTCCCAACTCCTTCAATGGCGCCAAAGGGAGAGAAGAAGCCCGCGGAGAAGAAACCCGTAGAGGAGAAGAAGTCTACAGTAGCCGAGAAATCTCCGGCTGAGAAGAAGCCAAAGGCCGGTAAGAAGCTACCGAAGGACGGTGGTGCCGCCGCCGTTGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAGACATACAAGATCTACATCTTTAAAGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCTATGGGAATCATGAACAGTTTCATTAACGATATCTTCGAGAAGCTCGCGGCTGAATCTTTCAGACTT
TARGET=221,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7125
PRIMER_LEFT_PENALTY=0.596759
PRIMER_RIGHT_PENALTY=0.115769
PRIMER_LEFT_SEQUENCE=GGAGAAGAAACCCGTAGAGGAG
PRIMER_RIGHT_SEQUENCE=CCCATAGCCTTGCTTGAGATAC
PRIMER_LEFT=102,22
PRIMER_RIGHT=326,22
PRIMER_LEFT_TM=60.597
PRIMER_RIGHT_TM=60.116
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.7125
PRIMER_LEFT_1_PENALTY=0.596759
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=GGAGAAGAAACCCGTAGAGGAG
PRIMER_RIGHT_1_SEQUENCE=CCATAGCCTTGCTTGAGATACC
PRIMER_LEFT_1=102,22
PRIMER_RIGHT_1=325,22
PRIMER_LEFT_1_TM=60.597
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.7441
PRIMER_LEFT_2_PENALTY=0.596759
PRIMER_RIGHT_2_PENALTY=0.147295
PRIMER_LEFT_2_SEQUENCE=GGAGAAGAAACCCGTAGAGGAG
PRIMER_RIGHT_2_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_2=102,22
PRIMER_RIGHT_2=313,22
PRIMER_LEFT_2_TM=60.597
PRIMER_RIGHT_2_TM=59.853
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.8089
PRIMER_LEFT_3_PENALTY=0.693167
PRIMER_RIGHT_3_PENALTY=0.115769
PRIMER_LEFT_3_SEQUENCE=CAAATTCCCAACTCCTTCAATG
PRIMER_RIGHT_3_SEQUENCE=CCCATAGCCTTGCTTGAGATAC
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=326,22
PRIMER_LEFT_3_TM=60.693
PRIMER_RIGHT_3_TM=60.116
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=5.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.8089
PRIMER_LEFT_4_PENALTY=0.693167
PRIMER_RIGHT_4_PENALTY=0.115769
PRIMER_LEFT_4_SEQUENCE=CAAATTCCCAACTCCTTCAATG
PRIMER_RIGHT_4_SEQUENCE=CCATAGCCTTGCTTGAGATACC
PRIMER_LEFT_4=54,22
PRIMER_RIGHT_4=325,22
PRIMER_LEFT_4_TM=60.693
PRIMER_RIGHT_4_TM=60.116
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_00037 Ps_contig_mira-and-tgicl-ass_franssen_700
SEQUENCE=CTTTAATTCAAAAATTTAAGACGAAACATACCCCAYTATAAACTTATAATAACTGAAAAATACATACATAACCAAAAACAAGAGGATTGATTTGATTTTAAAAGAAAGTTACACACTAATTCAGACCTAATTCTAGCACTTATACAAAATTCAAGCACTTGTAAACTTAGTGACAGCTTTCGTCCCTTCAGAAACAGCATGCTTAGCCAATTCTCCAGGCAGAACAAGCCTGACCGCAGTCTGAATTTCCCTCGATGTGATTGTTGGCTTCTTGTTGTACCTTGCAAGTCTGGAAGATTCAGCAGCAAGCTTCTCGAAGATGTCGTTGATGAAGCTGTTCATGATTCCCATGGCCTTGCTTGAGATACCGATGTCAGGGTGAACCTGCTTCAGAACTTTGAAGATGTAGATCTTGTATGTCTCCACGCTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCGGCGGCACCGGCTTCCTTGGGAAGCTTCTTTCCGGCTTTGGGTTTCTTCTCGGCAGGAGACTTCTCGGCCACCGTAGACTTCTTCTCTTCAACAGGTTTCTTCTCAGCGGGCTTCTTCTCTCCCTTTGGCGCCATTGAGAATTGAGAATTTGGAACTTTGAAGAAAAATTAGGGTTTCGGATTGGATATTAGAATTGGAAATCAAATATGATAGATGATCCCGACGTACTCTGCGTTG
TARGET=441,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00038 Ps_contig_mira-and-tgicl-ass_franssen_701
SEQUENCE=AGAGAAGTCGACGAGCGNGAGACNCGCNGAGAAAAGACAAGCGGAAGAAGCNGCTAAGGATGGTGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAGGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGAATCATGAACAGTTTCATCAACGACATATTCGAGAAGCTTGCTGCAGAATCTTCGAGGCTTGCAAGATACAACAAGAAGCCAACGATCACTTCCAGGGAAATTCAGACCGCGGTCAGGCTTGTTCTGCCCGGTGAATTGGCGAAGCATGCCGTTTCTGAGGGCACCAAAGCCGTAACCAAGTTCACAAGCTCTTAGACTTGAATAAATTAAGCTGCCAATTATCAGTTATTTCTATTTTTAGATCTTGTTTAGGTATCCTTAGAAAGTTTAATTTAGGGTTTTTTCTAGCTTTTAAAATGGTAGGCAAAGTAATGGATTCTGTTTCTATTAAAAGTGTAGACG
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3774
PRIMER_LEFT_PENALTY=0.347129
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=GAGAAAAGACAAGCGGAAGAAG
PRIMER_RIGHT_SEQUENCE=TGAACTTGCTTCAGAACCTTGA
PRIMER_LEFT=28,22
PRIMER_RIGHT=155,22
PRIMER_LEFT_TM=59.653
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=0.4070
PRIMER_LEFT_1_PENALTY=0.347129
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=GAGAAAAGACAAGCGGAAGAAG
PRIMER_RIGHT_1_SEQUENCE=TCTGAATTTCCCTGGAAGTGAT
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=295,22
PRIMER_LEFT_1_TM=59.653
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.4944
PRIMER_LEFT_2_PENALTY=0.347129
PRIMER_RIGHT_2_PENALTY=0.147295
PRIMER_LEFT_2_SEQUENCE=GAGAAAAGACAAGCGGAAGAAG
PRIMER_RIGHT_2_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=175,22
PRIMER_LEFT_2_TM=59.653
PRIMER_RIGHT_2_TM=59.853
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=0.5524
PRIMER_LEFT_3_PENALTY=0.347129
PRIMER_RIGHT_3_PENALTY=0.205226
PRIMER_LEFT_3_SEQUENCE=GAGAAAAGACAAGCGGAAGAAG
PRIMER_RIGHT_3_SEQUENCE=GGTGAACTTGCTTCAGAACCTT
PRIMER_LEFT_3=28,22
PRIMER_RIGHT_3=157,22
PRIMER_LEFT_3_TM=59.653
PRIMER_RIGHT_3_TM=59.795
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=130
PRIMER_PAIR_PENALTY_4=0.7222
PRIMER_LEFT_4_PENALTY=0.347129
PRIMER_RIGHT_4_PENALTY=0.375090
PRIMER_LEFT_4_SEQUENCE=GAGAAAAGACAAGCGGAAGAAG
PRIMER_RIGHT_4_SEQUENCE=TGAGATACCAATGTCAGGGTGA
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=174,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=60.375
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_00039 Ps_contig_mira-and-tgicl-ass_franssen_707
SEQUENCE=GKTCGGGGATTCATTCACATTTCAATCTTCCTTTTCAAAATTTCACAGTTGATAATTATAAGAAGAGAAAAATGTCAGGTCGCGGTAAAGGTGGAAAGGGTTTGGGAAAGGGAGGAGCAAAGAGGCACAGAAAAGTGCTTCGTGATAACATCCAGGGAATTACCAAGCCTGCAATTCGCCGTTTGGCGAGAAGAGGTGGTGTCAAGAGAATCAGTGGACTCATCTATGAAGAAACTCGTGGTGTTCTCAAGATCTTTCTCGAGAACGTTATTCGTGATGCCGTTACTTACACTGAGCACGCTAGGAGGAAAACTGTCACTGCCATGGATGTTGTTTATGCTCTCAAGAGGCAGGGAAGGACTCTGTACGGTTTCGGTGGTTGATTTTATGATCCAATTTAGGGTTTCGTTAGTGTTAGTTGGGTGTAGATGTATTTTATCTATTTAAGCTGAACTGAGGCAATTTGTTATGTTCTTCTATCATATGAACTTAATGATGGATATTCTGTTTTTCTGCTAAATATATCTTTGATTTGTTATCGTTTATGATYAAAAAACNGTAATCTCAATTAATTAATTAGAAACC
TARGET=567,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00040 Ps_contig_mira-and-tgicl-ass_franssen_724
SEQUENCE=CCTCTCTACAAATTTACGTAGATTCTTCTTCTTCAACCTCATCGCATCTCTAACCAACTTTTCTCCTAGCCATCACCGTAACAAACCTTCAACCGCTTCCAATCTTCACTCTTCAATTCTTCCGCATAACTGTATGATCATCACAAYCGAACCACCAAAGATCCTGCAACAATTCTGCARCAATTCTCTCCATTCTCGGAGGTGATAAGCAGAAGGAAAAAGAAGAAATAATAGAAAAGAGAAATCCAGAAGAGATCGGAATAATCGGAAACTCACGATCCAAGAGGCTTCTTCTTCATTTCGATCGGCAATACCGGTGTAACGTCTTCGTCTTCATCTCAAAGCGGAGACGCGAGCGAGGCCTTCATCATCCACCAAGAACCTTCAACAAAATCCGTAGAAGCTCGCAACTCATAACGGAATTGATGAGAAGCTCCAATCAGAAAGAAACCGAAAATTGAAGAAGCAGAAGAAGGATAGGAGCAGAGGAGCGATTGTTCATACCGAAGCAGTGTCTAAGCGCCGTCCGCCGTATGCTCCGTCGTCGACGTCGAAGGTACGCAAAGATTCTCCTATTTGACTCGTGTTGTCGACGTTGTAAAGGCATCGATTAGGTGAGCGTTTATCCCAAATTCATGGATTTGATTACCTCCATGCGAGGACTTAATTGAAGTTTTTTACCTAGGTTTCTTAAAATGATCTTTCTGGTTCGGTCAATAAAGAAGAAGTTGGAGTAGGAAATAGGGAGTAGATTCGTGGCAGGTGGTGGAAGCGAGAATGAGTGTGGTGAGTAATTCGCCGCTGTCGGTGATGAAATTCGGCGCGGCAAGGCTTTTCCGGTGAGGAGCGTTCTTTCATTTTTCTTCTGTGAGGTTAGCGAGCGATCCCCGCGTACTCTGCGTTGAACTACCACTGCTT
TARGET=23,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00041 Ps_contig_mira-and-tgicl-ass_franssen_728
SEQUENCE=AAATTTTTTAAAAATTCCATTTTCTCTTTTATCCTATTTATGCTAACCACATCCAATTTTAATTATCCACATTCAATTGAACCAATAGCTACCTACAAAACAACAATTCGCAACTCAAAGCTGCTACAGTTTAAGCCGAACACAACTTGAAACGCAGCCAAGGAATGAAACGTAAAGCAAGGCATCATGAGCTGCAAGCTACAACAACACCAAAACGCATAAGTCAGCTCCAAACGCACACCAGATGCTATGAACACAAGCCACCAAGCCAAACAACTTGCAAATCCATATTGAAACGCAGCTAACGCTTCCAAGCCGGTGAACCGCAAAGCAAAACGTGAGCCAACAACAAATTGCACAAATTAGCTCTCATAACTCTATAAAATCAATCTTCTTCTCATCTCCTAGATTCTTCTTCTTCAACCTCATCGCATCTCTAACCAACCTTTCTTCAGTTCTTTCAACCTTACA
TARGET=410,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3412
PRIMER_LEFT_PENALTY=0.124598
PRIMER_RIGHT_PENALTY=0.216588
PRIMER_LEFT_SEQUENCE=GAGCTGCAAGCTACAACAACAC
PRIMER_RIGHT_SEQUENCE=AGAAAGGTTGGTTAGAGATGCG
PRIMER_LEFT=188,22
PRIMER_RIGHT=450,22
PRIMER_LEFT_TM=60.125
PRIMER_RIGHT_TM=59.783
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=10.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.4236
PRIMER_LEFT_1_PENALTY=0.206979
PRIMER_RIGHT_1_PENALTY=0.216588
PRIMER_LEFT_1_SEQUENCE=GCAAGCTACAACAACACCAAAA
PRIMER_RIGHT_1_SEQUENCE=AGAAAGGTTGGTTAGAGATGCG
PRIMER_LEFT_1=193,22
PRIMER_RIGHT_1=450,22
PRIMER_LEFT_1_TM=60.207
PRIMER_RIGHT_1_TM=59.783
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=10.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.5133
PRIMER_LEFT_2_PENALTY=0.296664
PRIMER_RIGHT_2_PENALTY=0.216588
PRIMER_LEFT_2_SEQUENCE=ATATTGAAACGCAGCTAACGCT
PRIMER_RIGHT_2_SEQUENCE=AGAAAGGTTGGTTAGAGATGCG
PRIMER_LEFT_2=287,22
PRIMER_RIGHT_2=450,22
PRIMER_LEFT_2_TM=60.297
PRIMER_RIGHT_2_TM=59.783
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=10.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.5498
PRIMER_LEFT_3_PENALTY=0.333229
PRIMER_RIGHT_3_PENALTY=0.216588
PRIMER_LEFT_3_SEQUENCE=AGGAATGAAACGTAAAGCAAGG
PRIMER_RIGHT_3_SEQUENCE=AGAAAGGTTGGTTAGAGATGCG
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=450,22
PRIMER_LEFT_3_TM=59.667
PRIMER_RIGHT_3_TM=59.783
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=10.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.5498
PRIMER_LEFT_4_PENALTY=0.333229
PRIMER_RIGHT_4_PENALTY=0.216588
PRIMER_LEFT_4_SEQUENCE=AGCCAAGGAATGAAACGTAAAG
PRIMER_RIGHT_4_SEQUENCE=AGAAAGGTTGGTTAGAGATGCG
PRIMER_LEFT_4=155,22
PRIMER_RIGHT_4=450,22
PRIMER_LEFT_4_TM=59.667
PRIMER_RIGHT_4_TM=59.783
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=10.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_00042 Ps_contig_mira-and-tgicl-ass_franssen_733
SEQUENCE=GTTCGGGGTAAAGCAATATTAGTTTCATCTACAAAACAAGCAAAGAAAGAGAGARTGTTGAGATCCCAGGACTACCACAACCACATTGCAGGAGTCMAGGAAGGGAGCCAAGTGTTCTCTCCGACTTCGACCGCTACCCAAACGCTAGAAATGGCCTTACAACAAGGGTAGGGGTTTGTCTACGATGGAGGTCGGAGGGACTCCCCACCGCCACCACCACCACCACCACCCTGAGATCGTTGGAAGGTAGGGTTGAAGGTTGGGTAGGG
TARGET=211,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00043 Ps_contig_mira-and-tgicl-ass_franssen_734
SEQUENCE=AGCTTATGCTCTGTATGTTTTCCATTTTTGCAATCCAAGACCATTTGGCCTGTTCCTTCGTGGGTTATACTACAATACCAGCTGCTAAACAGAAGTTGAAATGCAGCATTACAAGCCAATGCCGACGCCGTAAACCAAACTCCAAACGTGAGCCAGCCATGAACAAAATTCTCTCCATCACTCTATAAATCAATCTTCACCCCTTCCAGATTCTTCTTCTTCAACCTCATCGCATCTCTAACCAACTTTTCNCCCA
TARGET=211,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6283
PRIMER_LEFT_PENALTY=0.237699
PRIMER_RIGHT_PENALTY=0.390638
PRIMER_LEFT_SEQUENCE=CCTGTTCCTTCGTGGGTTATAC
PRIMER_RIGHT_SEQUENCE=TGGGNGAAAAGTTGGTTAGAGA
PRIMER_LEFT=48,22
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=59.762
PRIMER_RIGHT_TM=60.391
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=4.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.6729
PRIMER_LEFT_1_PENALTY=0.237699
PRIMER_RIGHT_1_PENALTY=0.435201
PRIMER_LEFT_1_SEQUENCE=CCTGTTCCTTCGTGGGTTATAC
PRIMER_RIGHT_1_SEQUENCE=NGAAAAGTTGGTTAGAGATGCG
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=251,22
PRIMER_LEFT_1_TM=59.762
PRIMER_RIGHT_1_TM=59.565
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=4.6000
PRIMER_RIGHT_1_END_STABILITY=10.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.6973
PRIMER_LEFT_2_PENALTY=0.306698
PRIMER_RIGHT_2_PENALTY=0.390638
PRIMER_LEFT_2_SEQUENCE=CCAGCTGCTAAACAGAAGTTGA
PRIMER_RIGHT_2_SEQUENCE=TGGGNGAAAAGTTGGTTAGAGA
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=59.693
PRIMER_RIGHT_2_TM=60.391
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.7419
PRIMER_LEFT_3_PENALTY=0.306698
PRIMER_RIGHT_3_PENALTY=0.435201
PRIMER_LEFT_3_SEQUENCE=CCAGCTGCTAAACAGAAGTTGA
PRIMER_RIGHT_3_SEQUENCE=NGAAAAGTTGGTTAGAGATGCG
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=251,22
PRIMER_LEFT_3_TM=59.693
PRIMER_RIGHT_3_TM=59.565
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=10.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=1.2171
PRIMER_LEFT_4_PENALTY=0.826416
PRIMER_RIGHT_4_PENALTY=0.390638
PRIMER_LEFT_4_SEQUENCE=TAAACCAAACTCCAAACGTGAG
PRIMER_RIGHT_4_SEQUENCE=TGGGNGAAAAGTTGGTTAGAGA
PRIMER_LEFT_4=130,22
PRIMER_RIGHT_4=255,22
PRIMER_LEFT_4_TM=59.174
PRIMER_RIGHT_4_TM=60.391
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_00044 Ps_contig_mira-and-tgicl-ass_franssen_740
SEQUENCE=GATCCTTACACACATAAGGTTCAGCASACCGACGGCGGAATCGACGACGAACGRCGGTGGAAACGGCGGAGCAAGGACGACACAGCGAATCCCAAACCTCAGTGCTGAAACAACGGCGCCGTCTCTGGACGCGCAAAGCTTGACCTTCTCCAAATCGTGGCGCACCAAGCAAAGCAACACCAATTCGCAACTCAAAACCATCGTYGAACCTACCTTCTTGCTTCACCAGATTTAAACAGTAAAATCTAAATGTGGAGGTTGAATCGGAAAACTCACAAAACAGGTATCGCATATTTCTGCTCTGGTTTTCGCTCGATCCTTCTTCTTGAGCTATTCTGAGAGGGCTTTGCGTCGTTGTTGTTCGATGCGTTCTTTTGGTATTGAATCAAGATGAAGCACGTCACGTCGAGTTGCCGAATCGTGATGATTGACGAATCAAGATGGYGATGACGAGTTTGATTTCGTGGAGGATCGCTTCTACTTTTTCGGTTTCGATCTTCTCTCCTYCCGTCGAGGTTCTGYCTGAATTTTGTGAAGTTGCTTAGATTTTCGCAGACTATGGAGGATGGCGAATTGAAGACGTTGATGAGAGAGTGACAGGTTGTGGCTTTACTCCGGTTTCGGTTTCTGTTATATGAAGTTTGTTGAAGGAATTACGGATGGTTACAGGTCCAAGAAGATGATGGTGAAGGAGTTTGTGTGTGGTAGAGAATGAATACGAAGGGTAACGAATGAAGAAATTGGATCACGGTGGTGGTTGGTGAGGGTTGGATTCGATAGCGGTGGTGGTTCTTGGAAGGTTGAAGAAGATTAGGTTGAAGGCGGAGATTGTTACGGTGATGGCTGGGAGAAAGGTTGGTTAGAGATGCGATGAGGTTGAAGAAGAAGAATCTAGGAGATGAGAAGAAGATTGATTTTATAGAGCTAATTTGTGCAATTTGTTGTTGGCTCACGTTTTGCTTTGCGGTTCACCGGCTTGGAAGCGTTAGCTGCGTTTCAATATGGATTTGCAAGTTGTTTGGCTTGGTGGCTTGTGTTCATAGCATCTGGTGTGCGTTTGGAGCTGACTTATGCGTTTTGGTGTTGTTGTAGCTTGCAGCTCATGATGCCTTGTCCTTTTACGTTTCATTTCCTTGGCTTGTCGTTTMMAGTTGTGTTCGGACTTAAACTGTAGCAGTCTTTGAGTTGACGTTAAG
TARGET=879,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00045 Ps_contig_mira-and-tgicl-ass_franssen_764
SEQUENCE=TTTTCTATGTCGGTTTCATCATCCCATGGCTTCACATCCACAAAACAGATGATTTTCCTACTCTCTTCTTTTTTGTCCAGATGCCTTCACAGCTGCTGCCCGTTCCTCAGCTGCCTTCTTCTCTTCCTCAGTCTCTTCACCAAACAAATCCACATCATCATCATCGTCATCCTCTGCTTCGGTGGCCTTTGTGTCAGCAGCTGGGAGGAGTGGCAATGCCTTCTTCAGCCACAAGAGAAGACTTCCCACTAATGACCACCAGACTCCCCTCACCAAGAAACCACCAAGTAAATTCCTACAAACAAAGTCCAATCAATGTNTCTTCGACCCACCTAGACAAACTAGTT
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2504
PRIMER_LEFT_PENALTY=0.131699
PRIMER_RIGHT_PENALTY=0.118715
PRIMER_LEFT_SEQUENCE=TGCCTTCTTCTCTTCCTCAGTC
PRIMER_RIGHT_SEQUENCE=GTGGGTCGAAGANACATTGATT
PRIMER_LEFT=111,22
PRIMER_RIGHT=331,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=59.881
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.50
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.75
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.3144
PRIMER_LEFT_1_PENALTY=0.131699
PRIMER_RIGHT_1_PENALTY=0.182658
PRIMER_LEFT_1_SEQUENCE=TGCCTTCTTCTCTTCCTCAGTC
PRIMER_RIGHT_1_SEQUENCE=TGTCTAGGTGGGTCGAAGANAC
PRIMER_LEFT_1=111,22
PRIMER_RIGHT_1=338,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=60.183
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.50
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.0500
PRIMER_PAIR_1_COMPL_ANY=5.75
PRIMER_PAIR_1_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=0.3144
PRIMER_LEFT_2_PENALTY=0.131699
PRIMER_RIGHT_2_PENALTY=0.182658
PRIMER_LEFT_2_SEQUENCE=TGCCTTCTTCTCTTCCTCAGTC
PRIMER_RIGHT_2_SEQUENCE=GTCTAGGTGGGTCGAAGANACA
PRIMER_LEFT_2=111,22
PRIMER_RIGHT_2=337,22
PRIMER_LEFT_2_TM=60.132
PRIMER_RIGHT_2_TM=60.183
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.50
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.3500
PRIMER_PAIR_2_COMPL_ANY=5.75
PRIMER_PAIR_2_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=0.3376
PRIMER_LEFT_3_PENALTY=0.115965
PRIMER_RIGHT_3_PENALTY=0.221594
PRIMER_LEFT_3_SEQUENCE=TCCTCAGTCTCTTCACCAAACA
PRIMER_RIGHT_3_SEQUENCE=AGTGGGAAGTCTTCTCTTGTGG
PRIMER_LEFT_3=124,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=59.884
PRIMER_RIGHT_3_TM=59.778
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.3533
PRIMER_LEFT_4_PENALTY=0.131699
PRIMER_RIGHT_4_PENALTY=0.221594
PRIMER_LEFT_4_SEQUENCE=TGCCTTCTTCTCTTCCTCAGTC
PRIMER_RIGHT_4_SEQUENCE=AGTGGGAAGTCTTCTCTTGTGG
PRIMER_LEFT_4=111,22
PRIMER_RIGHT_4=249,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=59.778
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_00046 Ps_contig_mira-and-tgicl-ass_franssen_788
SEQUENCE=AAGCATGGTAGTCAACGCAGAGTASWCGGGACAACAAACTCTCCATTTCTATTCACACCCCCTACGCTCACATTAGCACAAAACCAAAATAGGCAACCGCAACAGCAACCGTCCGTCTTTGTTCCTTTACTTCATCCTTCATCGGAACTCGTCTCCGTGAAGTTCAATCCAATTCCGGCCGAGTACAAGCCAGATTCGGATTTGCATTACGGAAAGAAAGAAAAGACCGCACCAAAGAAACCATCACGCTCG
TARGET=212,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00047 Ps_contig_mira-and-tgicl-ass_franssen_796
SEQUENCE=TCGGGGATTCTAGAACACTTAAAACCCTAACTATATAAGCTAACTTCTACTGTACTTCGTTTTGCTCTTCACTTCTTCTTAGATCTGTGTCTCATTTATTTTCCGATTAGCCAAAAGGTAAAATCCAATGGCCGGAAAAGGAGAGGGACCGGCTATCGGAATCGATCTCGGAACCACCTACTCCTGTGTAGGAGTATGGCAACATGATAGAGTTGAAATCATTGCCAACGACCAAGGTAACAGAACTACACCGTCTTATGTCGCTTTCACTGACTCCGAGCGTCTCATCGGTGATGCCGCTAAGAACCAGGTCGCCATGAATCCTATCAACACCGTCTTCGATGCAAAGAGGTTGATTGGTCGTAGAGTCTCTGATGCATCTGTTCAGAGTGACATGAAGTTGTGGCCTTTTAAGATCATTGCTGGTCCTGCTGAGAAGCCAATGATTGTTGTTAACTACAAGGGTGAAGATAAGCAATTTGCTGCTGAAGAGATCTCTTCAATGGTTCTGATGAAGATGCGTGAGATTGCTGAAGCTTACCTTGGCTCTGCAATTAAGAACGCCGTTGTCACTGTCCCTGCTTACTTTAATGATTCTCAGCGTCAAGCTACCAAGGATGCTGGTGTTATTGCTGGTCTTAATGTCATGCGAATCATCAATGAGCCCACTGCTGCTGCCATTGCATATGGGCTAGACAAGAAAGCAACAAGTGTTGGTGAAAAGAATGTCTTGATTTTTGACCTTGGTGGTGGTACTTTTGATGTCTCTTTGCTTACTATTGAAGAGGGTATCTTTGAGGTGAAAGCCACAGCTGGAGACACCCATCTTGGAGGTGAGGATTTTGATAACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGAAAGAACAAGAAGGACATTAGTGGCAACCCCAGGGCACTCAGGCGATTGAGGACTGCATGTGAGAGGGCGAAGAGAACTCTTTCATCTACTGCCCAGACTACCATTGAAATTGACTCTCTATTTGAGGGAATTGATTTCTACTCCCCTATTACTCGTGCCAGATTTGAGGAGCTCAACATGGATCTCTTTAGGAAATGTATGGAACCAGTTGAGAAATGTTTGAGAGATGCCAAGATGGACAAAAAAAGCATCCATGATGTTGTTCTTGTTGGTGGTTCTACTAGAATTCCAAAGGTTCAACAACTTTTGCAGGATTTCTTTAATGGAAAGGAGCTATGCAAAAGCATTAATCCCGATGAGGCTGTTGCGTATGGAGCTGCTGTCCAGGCTGCAATTTTGAGTGGTGAGGGGAATGAGAAGGTGCAGGATCTTCTCCTTCTTGATGTTACCCCTCTCTCTCTTGGTTTGGAGACTGCTGGGGGTGTGATGACTGTTCTGATCCAAAGGAATACCACCATTCCAACAAAGAAAGAACAGGTTTTCTCAACCTATTCTGACAACCAGCCTGGTGTGTTGATCCAAGTCTTTGAAGGAGAGAGAACAAGGACTAGGGATAACAATTTGTTGGGCAAGTTTGAACTTTCTGGTATTCCTCCTGCTCCCAGAGGTGTTCCCCAAATTACTGTGTGCTTTGACATTGATGCCAACGGTATATTGAATGTCTCTGCTGAAGACAAGACTACCGGGCAGAAGAACAAGATCACCATCACAAATGACAAGGGTAGGCTTTCAAAGGATGATATTGAGAAGATGGTTCAAGAGGCTGAGAAGTACAAGTCTGAGGATGAAGAGCATAAGAAGAAGGTTGAAGCCAAGAATGCATTGGAAAACTATGCATACAACATGAGGAACACCATCAAGGATGACAAGATTGCAGGCAAACTTGATTCTGAAGACAAGAAGAAGATTGAGGACACCATTGAGCAGGCTATCCAGTGGTTAGACAGCAACCAACTTGCAGAGGCAGATGAGTTTGAGGACAAAATGAAGGAATTGGAAAGTGTCTGCAACCCTATCATTGCCAAGATGTACCAGGGTGGTGCAGGTCCTGACATGGGTGCTGCTGCAGATGATGATGCTCCTACTGGTGGAAGTGGTGCTGGCCCTAAGATTGAGGAAGTCGACTAAGTGTAGTTTCTTTCATCCTAACTATGATTTATATTTTCAAGTTATATTTAATTTGATGCATTTGCCGCTTATATTGTTTGCTTTTAGCTGGTTTTTGGAGCTATTTAGGCTACTTCGTTATGTTGTACTGTTAAGGATTGTGTTATTGTTATTGCAATTTTCATATACWATTAAAGGGTTTTAAACTTTTT
TARGET=1338,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00048 Ps_contig_mira-and-tgicl-ass_franssen_797
SEQUENCE=AACGAATGTACCAACGGTGGAGGCTGNCCTGACATGAGTGCAGATGATGATGAAGTTCCTCCAGCTGCAGGTGGTGCTGGTCCCAAAATTGAAGAAGTTGATTAAGTTTTTTCGTTTGATTGATTAGTCTCTTTTAATTTTACGCTTGTTTTAGTTAATTGGTCAGCGTTTTGGGGATTTCCTTTCTGATAATGTGATGTTGTGATGATTATTAGGTGTTTTGTTCGAATTTTGAATTGTTCATCTCAATTACAATTGGATGCTAAGTATTTGTATGAATTTTATTTTTTAATTGGAAGGAAGGAATTTTACTGGACBAACC
TARGET=295,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00049 Ps_contig_mira-and-tgicl-ass_franssen_814
SEQUENCE=AAGCAGTGGTATCAACGCAAGAAGTAACGTCGGGGATCAACAATCCAACCTTTCAGCAACACTGGTTACACACTTTACTCTTTTCACTCTCTCTCTCCATCACTTACGTTATCCTTATTCTTTGTTTTCCCACCACATCAATGGAAGTACTCTCTTCAAACAAAAGAAACCAACCTACCTCCTCGTTGAANCCCAACCGCTCCGATGTTCGTT
TARGET=87,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4404
PRIMER_LEFT_PENALTY=0.039879
PRIMER_RIGHT_PENALTY=0.400488
PRIMER_LEFT_SEQUENCE=AATCCAACCTTTCAGCAACACT
PRIMER_RIGHT_SEQUENCE=ACGAGGAGGTAGGTTGGTTTCT
PRIMER_LEFT=41,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=60.400
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=0.6038
PRIMER_LEFT_1_PENALTY=0.203343
PRIMER_RIGHT_1_PENALTY=0.400488
PRIMER_LEFT_1_SEQUENCE=CCTTTCAGCAACACTGGTTACA
PRIMER_RIGHT_1_SEQUENCE=ACGAGGAGGTAGGTTGGTTTCT
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=60.400
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=0.7817
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.741841
PRIMER_LEFT_2_SEQUENCE=AATCCAACCTTTCAGCAACACT
PRIMER_RIGHT_2_SEQUENCE=AACGAGGAGGTAGGTTGGTTTC
PRIMER_LEFT_2=41,22
PRIMER_RIGHT_2=186,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=60.742
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.8973
PRIMER_LEFT_3_PENALTY=0.039879
PRIMER_RIGHT_3_PENALTY=0.857451
PRIMER_LEFT_3_SEQUENCE=AATCCAACCTTTCAGCAACACT
PRIMER_RIGHT_3_SEQUENCE=CGAGGAGGTAGGTTGGTTTCTT
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=60.857
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=0.9452
PRIMER_LEFT_4_PENALTY=0.203343
PRIMER_RIGHT_4_PENALTY=0.741841
PRIMER_LEFT_4_SEQUENCE=CCTTTCAGCAACACTGGTTACA
PRIMER_RIGHT_4_SEQUENCE=AACGAGGAGGTAGGTTGGTTTC
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=186,22
PRIMER_LEFT_4_TM=60.203
PRIMER_RIGHT_4_TM=60.742
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_00050 Ps_contig_mira-and-tgicl-ass_franssen_815
SEQUENCE=CGGTTTAAACTTTTACGGTTTTTTACGGGTTACCCGTTTAGGGGGTACCCCCGTTTCCTAAACCCCCTAACGAAACGTTAGGCCTTCGTAAAAGACTTGAAAACTACACCACCACCCTCCAAGATRMYGGACGSKARWAGWTCAATTAAATGAGAAGCACGTCGATTCCGACAACACCGACGACACCGGTGACTCCGACAACACCTTCATCGGCGCGTAAAGTTGACAACGTTTGGAGGAGCGTGTTCAATCCTGGGAGTAACTCTGCTACAAAGTCAATTGGTGCTCATGTTTTCGACAAACCACTCCCCAACACCCCCACTGTCTATGATTGGATGTACAGTGGGGATACGAGAAGCAAGCACCGTTGATTAGTGATCAAGTGGATACCAACTCCCTTGGGTTTTGTCATGTAAATAGTGTTCTTGTTTTGTTTCTATCTTCTGCCACGTGTCTACATCACCGTTACGTGTTGAGATCTGTAGTTGGTTTGGTTTGGTCGAGTAGGTTTTAGTTTTTTTTTTAAGTTTTTTAAGTTAC
TARGET=486,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00051 Ps_contig_mira-and-tgicl-ass_franssen_816
SEQUENCE=GAACACTCTTATTACATAAATTTAACTCATCAAACTCTTTTATCAAACAAACAAAATGTTAGATAAACTTTGGGATGATGTTGTTGCTGGCCCTCAACCAGAACGTGGCCTTGAAAGCTTAGAAACTCACCACCACCCTCAAAGATGATGGAAGATGGTGCTACTTATTCTCAGGCTGAACCAACAAAACCNGTATGGTGTGAGAGAAAACAGCCGTAGACAAGAAGAAATATGGANAATGAATCAATTACCTGACTAAAAACG
TARGET=44,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00052 Ps_contig_mira-and-tgicl-ass_franssen_825
SEQUENCE=AGAGAAGGAGGAATATGAAGCGGCCCGGCGCGAATTATTGGAAGAAGTAGCTAGGGGTACCTCTGTACAAGATATTCGTGCAAGGTTGACTAACAAAACTAGTGATGTTGAAGTAAAGGAGCCTTCTGTTTCGAAAACAAAGACCATCCCTGATGAACTTGTACAGATTCAAGCATTTATACGCTGGGAAAAAGCTGGGAAGCCTAATTATTCTCCCGAACAACAACTTATTGAATTTGAGGAAGCAAGAARAGGATTGCTAAAGCAAAATCCGAGGCCGCAGAGTACCAAAAACTTCTTGCCTCCAGATTGAAGGAGCAGAGGGACCGCAGAAGTGAGAGTTTGGTTAAGAAGAGATCAAGGCTCTCTACCGCCAAGCCTGTTGCAGCATAGGTCTTTCTTTGCAACAAATGAAGTTCATACTTCGGCCGGTTAGTTTTTGTTAAGTTATTTATTTATTTTTTGGTTCG
TARGET=448,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00053 Ps_contig_mira-and-tgicl-ass_franssen_834
SEQUENCE=AATTCCAACAGCATTCATGGATTTATTACTTAGGACCCAAACGTCCCTTTGTAGGGCCACAAATCTGCTCCTCGCCCATGGCAGACATGACAGACACAATCAGATCTTTACCTTCGTTGAATCCATCCTTGATCTGCGAAAGCAGACTATCATCGGACGGAAGCTTAAGGTCATCCTTTGTACCACCGCTCTCAGTCAGCAGACTCACAAATCCATCCTCTGAGATATCAATAAGCTGGTAATCAGTACGATTGACATGAGGAACATCACTAGTTATGAAGAAGAAGAACAATNTCTNATCTTTGCNGTAGAAGTNTT
TARGET=278,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00054 Ps_contig_mira-and-tgicl-ass_franssen_839
SEQUENCE=AAGCAGGGTAGTCAACGACAGAGTACGCGGGGTGGTGAAGAACAAGAAGTAAAACAATGTCTGACGAGGAACACCATTTCGAGTCCAAAGCTGATGCTGGAGCATCCAAGACATATAACCAAACAAACAAAGCTGGAACCATTCGACAAAAATGGTCACATTGTTATACAAAAACAGACCCTGCAAGGTTGTTGTAGGTTCAACTTACAAAAAC
TARGET=120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5645
PRIMER_LEFT_PENALTY=0.361206
PRIMER_RIGHT_PENALTY=0.203343
PRIMER_LEFT_SEQUENCE=AAACAATGTCTGACGAGGAACA
PRIMER_RIGHT_SEQUENCE=TGAACCTACAACAACCTTGCAG
PRIMER_LEFT=51,22
PRIMER_RIGHT=201,22
PRIMER_LEFT_TM=59.639
PRIMER_RIGHT_TM=60.203
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=0.9430
PRIMER_LEFT_1_PENALTY=0.361206
PRIMER_RIGHT_1_PENALTY=0.581808
PRIMER_LEFT_1_SEQUENCE=AAACAATGTCTGACGAGGAACA
PRIMER_RIGHT_1_SEQUENCE=GACCATTTTTGTCGAATGGTTC
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=156,22
PRIMER_LEFT_1_TM=59.639
PRIMER_RIGHT_1_TM=60.582
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=0.9450
PRIMER_LEFT_2_PENALTY=0.741655
PRIMER_RIGHT_2_PENALTY=0.203343
PRIMER_LEFT_2_SEQUENCE=GCAGGGTAGTCAACGACAGAGT
PRIMER_RIGHT_2_SEQUENCE=TGAACCTACAACAACCTTGCAG
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=201,22
PRIMER_LEFT_2_TM=60.742
PRIMER_RIGHT_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=1.0100
PRIMER_LEFT_3_PENALTY=0.806635
PRIMER_RIGHT_3_PENALTY=0.203343
PRIMER_LEFT_3_SEQUENCE=ATGTCTGACGAGGAACACCATT
PRIMER_RIGHT_3_SEQUENCE=TGAACCTACAACAACCTTGCAG
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=201,22
PRIMER_LEFT_3_TM=60.807
PRIMER_RIGHT_3_TM=60.203
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=1.0100
PRIMER_LEFT_4_PENALTY=0.806635
PRIMER_RIGHT_4_PENALTY=0.203343
PRIMER_LEFT_4_SEQUENCE=AATGTCTGACGAGGAACACCAT
PRIMER_RIGHT_4_SEQUENCE=TGAACCTACAACAACCTTGCAG
PRIMER_LEFT_4=55,22
PRIMER_RIGHT_4=201,22
PRIMER_LEFT_4_TM=60.807
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_00055 Ps_contig_mira-and-tgicl-ass_franssen_851
SEQUENCE=CAATNAGGAGCTAGAGAAAATTAGATGGTTCTGTTTTCTCAATACAAGTTTAGAAGAAAAAAATAAAGCAAGAAGTGTACAAAATTAACAATGGCACATGGTCGTACATATATATATAGATATAGATATAATTAACAAAACCTTATTTTGGCTCTTACTTCCAGTTATTGGCAACAATGTTCAGCCAAATCAACAACCCTTTGTGAGTAACCCCACTCATTATCATACCAAGCAATCACCTTAACCAAGTCATCGCCCATGACCATTGTCACCGCCACCGATGTAAACCAAGGTACTGAATCTATCCAACCAACAAACATGGATCAGCAAACTGCTGTAGATGAGTCTGTTAAACCCAGTTCAACTACATCAGTTCTTTGTGAACTCAGAACCATTGGCGGGAAAAAA
TARGET=108,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1733
PRIMER_LEFT_PENALTY=1.135941
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=TAACAATGGCACATGGTCGTA
PRIMER_RIGHT_SEQUENCE=CAGTTTGCTGATCCATGTTTGT
PRIMER_LEFT=85,21
PRIMER_RIGHT=333,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=1.1953
PRIMER_LEFT_1_PENALTY=1.157991
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=TTAACAATGGCACATGGTCGTA
PRIMER_RIGHT_1_SEQUENCE=CAGTTTGCTGATCCATGTTTGT
PRIMER_LEFT_1=84,22
PRIMER_RIGHT_1=333,22
PRIMER_LEFT_1_TM=61.158
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=1.4117
PRIMER_LEFT_2_PENALTY=1.135941
PRIMER_RIGHT_2_PENALTY=0.275749
PRIMER_LEFT_2_SEQUENCE=TAACAATGGCACATGGTCGTA
PRIMER_RIGHT_2_SEQUENCE=TCCATGTTTGTTGGTTGGATAG
PRIMER_LEFT_2=85,21
PRIMER_RIGHT_2=322,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.724
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=1.4117
PRIMER_LEFT_3_PENALTY=1.135941
PRIMER_RIGHT_3_PENALTY=0.275749
PRIMER_LEFT_3_SEQUENCE=TAACAATGGCACATGGTCGTA
PRIMER_RIGHT_3_SEQUENCE=CCATGTTTGTTGGTTGGATAGA
PRIMER_LEFT_3=85,21
PRIMER_RIGHT_3=321,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.724
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=1.4303
PRIMER_LEFT_4_PENALTY=1.392962
PRIMER_RIGHT_4_PENALTY=0.037339
PRIMER_LEFT_4_SEQUENCE=ATTAACAATGGCACATGGTCGT
PRIMER_RIGHT_4_SEQUENCE=CAGTTTGCTGATCCATGTTTGT
PRIMER_LEFT_4=83,22
PRIMER_RIGHT_4=333,22
PRIMER_LEFT_4_TM=61.393
PRIMER_RIGHT_4_TM=60.037
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_00056 Ps_contig_mira-and-tgicl-ass_franssen_859
SEQUENCE=CCGTTTTCGGTTCTTCGTTGGAGGTTTTGGTTCTAGTGGAGTGATTATTNACAGAGCTGTTGCGAAATACTGAAATTTAGTCAGGTTCAAATGCTTATTCAAGTGTAAGTGTTGCTTGGTTGGTTGGTTAGTTAGAAGTGAGGAAGAAAGATTGCAGGGTTAGCTTCGAGGGTTCAAGAGAAGGGGTAAAAGGGCCGTTGAGTATCTACTGCTGAGGTATTCGAGTTAACCCCGTCTTTGGTTGTTGTTGAAGTGAAGAAAAAAGGAGGGGATAAAGTTGAGTATGATAGGTTTTTAAACACTGAATTGAAGCCTGCTTTGCATAGTTTAACCATGGAAGAATCTGCAGGTTCTTCATGTCAAAATACACCAGAGGAAACTTTGCAACAACGCGTCGTTTTCTGATTCCGCCATTGACAAACNTTCAGATAGCATTGAATCTCTA
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4912
PRIMER_LEFT_PENALTY=0.376728
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=TTGGAGGTTTTGGTTCTAGTGG
PRIMER_RIGHT_SEQUENCE=CCTGCAATCTTTCTTCCTCACT
PRIMER_LEFT=17,22
PRIMER_RIGHT=157,22
PRIMER_LEFT_TM=60.377
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.5297
PRIMER_LEFT_1_PENALTY=0.415225
PRIMER_RIGHT_1_PENALTY=0.114470
PRIMER_LEFT_1_SEQUENCE=ACAGAGCTGTTGCGAAATACTG
PRIMER_RIGHT_1_SEQUENCE=CCTGCAATCTTTCTTCCTCACT
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=157,22
PRIMER_LEFT_1_TM=59.585
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=108
PRIMER_PAIR_PENALTY_2=0.5546
PRIMER_LEFT_2_PENALTY=0.440098
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=CGTTGGAGGTTTTGGTTCTAGT
PRIMER_RIGHT_2_SEQUENCE=CCTGCAATCTTTCTTCCTCACT
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=157,22
PRIMER_LEFT_2_TM=59.560
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=143
PRIMER_PAIR_PENALTY_3=0.5693
PRIMER_LEFT_3_PENALTY=0.415225
PRIMER_RIGHT_3_PENALTY=0.154110
PRIMER_LEFT_3_SEQUENCE=ACAGAGCTGTTGCGAAATACTG
PRIMER_RIGHT_3_SEQUENCE=GGTTAAACTATGCAAAGCAGGC
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=332,22
PRIMER_LEFT_3_TM=59.585
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.5737
PRIMER_LEFT_4_PENALTY=0.459263
PRIMER_RIGHT_4_PENALTY=0.114470
PRIMER_LEFT_4_SEQUENCE=GAGCTGTTGCGAAATACTGAAA
PRIMER_RIGHT_4_SEQUENCE=CCTGCAATCTTTCTTCCTCACT
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=157,22
PRIMER_LEFT_4_TM=59.541
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00057 Ps_contig_mira-and-tgicl-ass_franssen_892
SEQUENCE=CCCCTCCGTTGTCGTGGTCGTCNGAAGACANGGTAGTCAACGACCACGAGTAACGCGGGGAGGTCCACTCCAATCATAATAATACTTTCCTTGTTTCTAACCAGAAAAAAACGAAACAASTCGGGGGATAGATCACTCAATCACTGCATATACATATCTTCATTGAATTAATCAACATTACTCTCTCTTCCTCCATCTTCTAGATTCTTCCATCATCAACAACAACAACAAACATGGCTGCTGCAACCGTTTATCA
TARGET=217,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00058 Ps_contig_mira-and-tgicl-ass_franssen_895
SEQUENCE=TGATGGTGCCTACCCTTCCACTTATAGAAACTTATTTGTTTTCTAATGAAACCTTTTTGAAGACAAGTCTCTCTGGCGTKCTAACAATAGATGTTCTGATCGGAAGGGAAGTGGAGTTGGAGTATGGCAAGGAGACTGCGGCTGTTACTGCAGCAGCCATGTTTGTTGTTGTTGTTGATGATGGAAGAATCTAGAAGNTGGAGGAAGAGAGAGTAATGTTGATTAATTCAATGAAGATATGTATATGCAGTGATTGAGTCCCTGCGTACTCTGCGTTGATA
TARGET=163,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00059 Ps_contig_mira-and-tgicl-ass_franssen_901
SEQUENCE=AGGTCTAAGAGGTAAAAGAAAGTACTAGTAGTAGTAGTAAGTTTACCTTCCACTTAGTTTCTTACCGTAAGACCCCCTCCCGGACGTACAGCATTGTAAGAACGTTCGGTTCCAAACCGGTAGGGAAGTACAAGAAGTTCAGATYWGYTKCAATTCTTTGCAGTGCGCTCGCAAGCCATCCTGATATACCGTCCTGATTCTCGTTCCCGTGCATGCAAGCACTTTTCTATTAGCTTTTTTTGTTTTCCTTTAGCTTTTTTCACTGCTCTCCTTCAAACAACATCTTTCCAAATCCTCTCCTTGCCACTATAATTTTTGTTTCTCTCCTTGTTTCTTTTGTATTTCTTTCTGTTTGAACTGTGGCCATGGCAGTTTCTGCAATTGGTTTTGAAGGTTTCGAAAAGAGGTTGGAAATATCCTTTTCTGACCCAGGACTTTTCTCTGACCCTCAAGGAAGGGGATACGATCTCTCACAAATCCCAATTGGACGAGATTCTTGCACCNGCTGAGTGCCACTATTTGTTTCCTTCCCTTGACTAAAGTGGAGGTATTGGTTTAGTACTTT
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00060 Ps_contig_mira-and-tgicl-ass_franssen_907
SEQUENCE=GGKTTCCGTCTGATCGAGTGTGGTTGTGGGGTTTTAGGGCGCAATCTTCTTTAGCTAGCACGCAGAGCAGACGCAACCTTCCTCACCTCTCTTCCATTCACGATGGCCGRCGATGACTACGAAGATATCGACATGGGATACGAAGAGGAGCCAGCGGAGCCTAGARATCGAGGAAGGCGCAGGAGGAGGAGGATCCAGAATAAACAAAAACGNGTRGWSWYRGAWTAGGG
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00061 Ps_contig_mira-and-tgicl-ass_franssen_910
SEQUENCE=CCTTTTCCCTAAACTTCCCTTCCTTCCTTGTCCACTTATTAATTTTTGTTTTCTTCTTCCTTTGTTTTCTTTTTGTTATTTTCTTTTCTTGTTTTGTAACTTGTTGGCCATGGCAGTTTCTGCAATTGGTTTTGAAGGTTTCGAAAAGAGGTTGGAAATATCCTTTTCTGACCCAGGACTTTTCTCTGACCCTCAAGGAAGGGGATTACGATCTCTCACAAAATCCCAATTGGTACGAGATTCTTGCACCAGCTGAGTGCACCATTGTTTCTTCCYTTGCAAATGAGGATGTTGATTCCTATGTTCTATCTGAGTCCAGCCTTTTTTGTTTATGCCTACAAGCTCATAATCAAACCTGCGGCACTACCAAGTTACTGCTTTACAATCCCAACAATTATTAGAAAATTTCGGGCCCGAAACCTTCCCTAATTTTTCCTCTTTAAATTGGTTTAAGATTCTTGGTTAGGAAGGGTACCCACCCCGGAGGGCCTACG
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00062 Ps_contig_mira-and-tgicl-ass_franssen_934
SEQUENCE=TACACTCCCTTCACGGTTACCTCAGACTTCTTACAAATCTAGTTTCCACCGATTCCACCTCCCTTCATTTTGAGTATGTCGTTTTTTTATTCTCTTGCACAACCTTCACAATGCATGTGAACTTTAAGAACAACCGTTACAATCTGAGGCTCTTCTTTCTTCTCCTCTTCAGGTTTCTTGGTTTTCTCTTCTTCCTCAACAACTTTTTCCTCCTCTGGTGGATTCGGGAGCGGAGATACAAGCTCAACCTTCCTCCGACTCTTCGTCTGAATTCTCTCCACACCTTCGCCGGNTCAGTCTTTCCTCCTACCTTTCAACCAACCAACCTCATTGTTGTTTTGCTAGTTTAGTTGTCCACCTACCCTTACCAACCGGTACCGGGTAATTCTACAACAGCGACGTACTTCCGANCCACACGTCCTTACAACAATACG
TARGET=315,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2048
PRIMER_LEFT_PENALTY=0.060824
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=ACAACCGTTACAATCTGAGGCT
PRIMER_RIGHT_SEQUENCE=GTTGGTAAGGGTAGGTGGACAA
PRIMER_LEFT=129,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.2048
PRIMER_LEFT_1_PENALTY=0.060824
PRIMER_RIGHT_1_PENALTY=0.143968
PRIMER_LEFT_1_SEQUENCE=ACAACCGTTACAATCTGAGGCT
PRIMER_RIGHT_1_SEQUENCE=TTGGTAAGGGTAGGTGGACAAC
PRIMER_LEFT_1=129,22
PRIMER_RIGHT_1=370,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.2808
PRIMER_LEFT_2_PENALTY=0.136881
PRIMER_RIGHT_2_PENALTY=0.143968
PRIMER_LEFT_2_SEQUENCE=CAACAACTTTTTCCTCCTCTGG
PRIMER_RIGHT_2_SEQUENCE=GTTGGTAAGGGTAGGTGGACAA
PRIMER_LEFT_2=196,22
PRIMER_RIGHT_2=371,22
PRIMER_LEFT_2_TM=60.137
PRIMER_RIGHT_2_TM=60.144
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=176
PRIMER_PAIR_PENALTY_3=0.2808
PRIMER_LEFT_3_PENALTY=0.136881
PRIMER_RIGHT_3_PENALTY=0.143968
PRIMER_LEFT_3_SEQUENCE=CAACAACTTTTTCCTCCTCTGG
PRIMER_RIGHT_3_SEQUENCE=TTGGTAAGGGTAGGTGGACAAC
PRIMER_LEFT_3=196,22
PRIMER_RIGHT_3=370,22
PRIMER_LEFT_3_TM=60.137
PRIMER_RIGHT_3_TM=60.144
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.2861
PRIMER_LEFT_4_PENALTY=0.142170
PRIMER_RIGHT_4_PENALTY=0.143968
PRIMER_LEFT_4_SEQUENCE=TTCTCCTCTTCAGGTTTCTTGG
PRIMER_RIGHT_4_SEQUENCE=GTTGGTAAGGGTAGGTGGACAA
PRIMER_LEFT_4=159,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=59.858
PRIMER_RIGHT_4_TM=60.144
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_00063 Ps_contig_mira-and-tgicl-ass_franssen_935
SEQUENCE=CTGCATGTCCTCCATACTATTCTTTAAACTTATTATTCTTTCCACCAGCACCGCAGATCTTCAGCGACGAGAATCCAAATGCTTGCAGTGTTATGTGAAATTTAATTTCACAAATATCAAGGGTCATATTAAAAATAAGAGATAGTAATTAGTTTTATTTTGCTAAATATACTAGAGTATACATGAACCATGGWAWTTTTTATTTTGTAAKATTTTTATACTTTTGTTGAAGTGAGTAATTTCTTATTTTKKTTTAAAGGCATGTGAGAAGTTAGGAAAGTTTGTTATGATATAACAAACAAACAAGCATCTATCTTAGATAGGAAANCTATTTATGTTACTTTTTAAAGTTTT
TARGET=294,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00064 Ps_contig_mira-and-tgicl-ass_franssen_965
SEQUENCE=CAGCCTGATCAGGCAAGGATTTTAGGGTTTTGAATGCTTCGTACTACCTCAAAGCTGGAGGTCATTTTTGTTTATTTTCTTATTCAAGGCCAATTGTATCGATTCCACAGTCCCTGCTGAGGCAGTGTTCACCAGTGAAGTGAACAAGTTGAAAGCAGATCARATTTAAACCATTTGAGCAAGTGACTCTTGAACCTATTTGAGGAGGGACCATGCTTGTGTTGTTGGTGGCTACAGAATGCTAAGAAGAAAGAAAGATGCTAGAAGTTAACCTTTATTTTTGTTTAGTTTTAAAATTAGTTACTTATTTGTTA
TARGET=247,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00065 Ps_contig_mira-and-tgicl-ass_franssen_979
SEQUENCE=CAACTCCTCAATAACATCTTCATTGAAATTTTCTTTCTTTCCCTTTTGAATGTTCTTCTCTTACCTCAGTTTGTAGCTTTTGCTAATACACTTGGGTTTCTTATCATTTCTGT
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00066 Ps_contig_mira-and-tgicl-ass_franssen_999
SEQUENCE=CGAAACCCCGTAAAACCTAACGGAAGTCAGGTCGTGAGCTATCTGGTTGGGTTGGGGGCGGATTCTGTTCGGTTTTTAGGAGTTAAAGGTTCGTATGTCTGAATTAGACGCACAAACTACCTACCTACCTTTGTATCCTTTTGTCTGAGGCAAATAGCTGAGGACGCCGGTGCTGGCTCATCAAAGGAGTACGTGCATATTCGTATACAGCAGCGAAATGGTAGAAAAAGCTTGACAACTGTCCAGGGTTATTTGAAGAAAGAATTCAGTTACAACAAAGATTCTTAAAGACCTTTAAGAAGGAGTTTTGCTGTAATGGTACAGTTGTCCTTAGGACCCAGAGCAAGGACAGGTTATTCAACTTCAAGGTGATCAGAGGAAGAATTGTTTCTACCTTCCTAGTCCAGGCTGGAATTGTGAAGAAGGAGCATTAATTAAAATCATGGTTTMYAAGCTGCGTAGGAAACTTGGAAAGTTGTTTAAGTTTGTATCGTGGCCCACATCGG
TARGET=117,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00067 Ps_contig_mira-and-tgicl-ass_franssen_1001
SEQUENCE=GGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGANCTCGAGAGCACTACAGAGAAGAATTAMACCACCTGACTGGCTATTATTGTTTGATATAACNTGTGTAATGAATGAGTTGACTAACTTTAGGGTTAATTTTGGWAGGGTTTCGTTTTTAACCTTTAACCCTAACAAATTAAAACGTTAAAAA
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00068 Ps_contig_mira-and-tgicl-ass_franssen_1002
SEQUENCE=GGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTAGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTATTATTGTTGATATAACNTGTAGTAATGTAATGAGTTGCYAACTTTAGGGTTATTTKGRAGGGTTTCGTTTTTAACCTTAACCCTAACAAAGTTAAAACGTTAAAAA
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00069 Ps_contig_mira-and-tgicl-ass_franssen_1003
SEQUENCE=GGGATGACRAGGAGGAAGGTTAAAGTAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTATTTATTGTTTGATATAACATGTGTAATGATGAGTTGCAACTTAGGTTATTTGGAGGTTGTTTTACCTTACCTAACATAAAGTAAAA
TARGET=56,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00070 Ps_contig_mira-and-tgicl-ass_franssen_1004
SEQUENCE=GGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTTATTATTGTTTGATTAATAACATGTTAGTAATGAATGAGTTGACTAACGTTTAGGTTATTTGGAGGKTTWCGTTTTTAACCTTAACCCTAACAATTAAAACGGTTAAAA
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00071 Ps_contig_mira-and-tgicl-ass_franssen_1005
SEQUENCE=GKGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAGGRNCTCGAGAGCACTACAGAGAAGAATTAACCNCCTGCTGGCTATTATTGWTTGWTAATAAACAATGTAGTAANGAATGAGTTTGACCTAAACGTTTAGGTTATTTGGAGGTTCTTTACCTACCTANAATTAAAACG
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00072 Ps_contig_mira-and-tgicl-ass_franssen_1006
SEQUENCE=GGGTATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTRGTGACGATTTCTCAGTTCAGTCTGAGAAGGGNCTCGAGAGCACTACAGAGAAGAATTAAACCNCCTGCTGGCTTATTATTGTTNATANAACNTGTAGTAATGAATGAGTT
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00073 Ps_contig_mira-and-tgicl-ass_franssen_1007
SEQUENCE=AAAAGAACAAGTGGTATCAACGCAGAGTGGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTATTATTGTTTGATATAACATGTGTAATGAATGAGTTGCTAAACTTTAGGGTTATTTTGGAGGGTTTCGTTTTTAACCTTAACCCTAACTAAATTAAAACGTTAAAAACC
TARGET=82,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1311
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT=52,22
PRIMER_RIGHT=167,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=0.1311
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=167,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.1764
PRIMER_LEFT_2_PENALTY=0.175676
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=GAACAAGTGGTATCAACGCAGA
PRIMER_RIGHT_2_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=167,22
PRIMER_LEFT_2_TM=60.176
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.2112
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.080887
PRIMER_LEFT_3_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_3_SEQUENCE=ACAATAATAGCCAGCAGGTGGT
PRIMER_LEFT_3=52,22
PRIMER_RIGHT_3=177,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=59.919
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.2112
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.080887
PRIMER_LEFT_4_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_4_SEQUENCE=ACAATAATAGCCAGCAGGTGGT
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=177,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.919
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=127
=
PRIMER_SEQUENCE_ID=SSR_00074 Ps_contig_mira-and-tgicl-ass_franssen_1008
SEQUENCE=GGGTATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAGGGGCTCGAGAGCACTACAGAGAAAGTAAACTTAAACCCACCTGCTGGCTATTNTTGTTGATATAAC
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8133
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.682954
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=TCAACAANAATAGCCAGCAGGT
PRIMER_LEFT=25,22
PRIMER_RIGHT=156,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.683
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.8133
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.682954
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=TCAACAANAATAGCCAGCAGGT
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=156,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.683
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=1.1996
PRIMER_LEFT_2_PENALTY=0.516652
PRIMER_RIGHT_2_PENALTY=0.682954
PRIMER_LEFT_2_SEQUENCE=ATAGCTCAACAGTGGGAGGATG
PRIMER_RIGHT_2_SEQUENCE=TCAACAANAATAGCCAGCAGGT
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=156,22
PRIMER_LEFT_2_TM=60.517
PRIMER_RIGHT_2_TM=60.683
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=130
PRIMER_PAIR_PENALTY_3=1.3346
PRIMER_LEFT_3_PENALTY=0.651681
PRIMER_RIGHT_3_PENALTY=0.682954
PRIMER_LEFT_3_SEQUENCE=GGGTATGACAAGGAGGAAGGTA
PRIMER_RIGHT_3_SEQUENCE=TCAACAANAATAGCCAGCAGGT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=156,22
PRIMER_LEFT_3_TM=59.348
PRIMER_RIGHT_3_TM=60.683
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=1.5210
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=1.390638
PRIMER_LEFT_4_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_4_SEQUENCE=CCAGCAGGTGGGTTTAAGTTT
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=143,21
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=60.391
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_00075 Ps_contig_mira-and-tgicl-ass_franssen_1009
SEQUENCE=GGGGTATTGTACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGAGCTCGAGAGCACTACAGAGAAGAATTAACCACCTGCTGGCTTATTATTGTTAGATATAACNTGTNTAATGAATGAGTTGACTAACGTTAGGTATTTGGAGGTTNTTACTACCANATAAGTAA
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4775
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.347129
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=CCAGCAGGTGGTTAATTCTTCT
PRIMER_LEFT=28,22
PRIMER_RIGHT=141,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=59.653
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=0.4775
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.347129
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=CCAGCAGGTGGTTAATTCTTCT
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=141,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.653
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=0.5133
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.382951
PRIMER_LEFT_2_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_2_SEQUENCE=CAATAATAAGCCAGCAGGTGGT
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=151,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=60.383
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=0.5133
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.382951
PRIMER_LEFT_3_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_3_SEQUENCE=CAATAATAAGCCAGCAGGTGGT
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=151,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=60.383
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=0.6197
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.489375
PRIMER_LEFT_4_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_4_SEQUENCE=TAAGCCAGCAGGTGGTTAATTC
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=145,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=60.489
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_00076 Ps_contig_mira-and-tgicl-ass_franssen_1010
SEQUENCE=AAGCMGTGGTATCAACGCAGAGTGGGATGACTAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAAGAGAAGAATTAAACCCACCTGCTGGCTTATTTATTTGTTTGATATAACNTGTNTAATGAATGTAGTTGACTAACGTTTAGGTTATTTTAGGTAGGTTTACGTTTTACCTTACCCTAACTATAAAGTAAACTTAAA
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00077 Ps_contig_mira-and-tgicl-ass_franssen_1011
SEQUENCE=GGGTATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGANCTCGAGAGCACTACAGAGAAGAATTAAACCNCCTGCTGGCTTATTTATTGTTTGAATAATAACNTGTGTAATGAATGAGTTGACTAACTTTAGGTTATTTTGGAACGGGTTTTACGTTTTTTAAACCCTTTAACCCTAAC
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1988
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.068420
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=ACAATAAATAAGCCAGCAGGNG
PRIMER_LEFT=25,22
PRIMER_RIGHT=152,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=59.932
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.5250
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=0.1988
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.068420
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=ACAATAAATAAGCCAGCAGGNG
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=152,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.932
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.5250
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.5851
PRIMER_LEFT_2_PENALTY=0.516652
PRIMER_RIGHT_2_PENALTY=0.068420
PRIMER_LEFT_2_SEQUENCE=ATAGCTCAACAGTGGGAGGATG
PRIMER_RIGHT_2_SEQUENCE=ACAATAAATAAGCCAGCAGGNG
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=152,22
PRIMER_LEFT_2_TM=60.517
PRIMER_RIGHT_2_TM=59.932
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.5250
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.7201
PRIMER_LEFT_3_PENALTY=0.651681
PRIMER_RIGHT_3_PENALTY=0.068420
PRIMER_LEFT_3_SEQUENCE=GGGTATGACAAGGAGGAAGGTA
PRIMER_RIGHT_3_SEQUENCE=ACAATAAATAAGCCAGCAGGNG
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=152,22
PRIMER_LEFT_3_TM=59.348
PRIMER_RIGHT_3_TM=59.932
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.5250
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=153
PRIMER_PAIR_PENALTY_4=0.7353
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.604956
PRIMER_LEFT_4_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_4_SEQUENCE=AATAAATAAGCCAGCAGGNGGT
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=150,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=60.605
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.50
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.2250
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_00078 Ps_contig_mira-and-tgicl-ass_franssen_1012
SEQUENCE=GGGTATTGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGTAACTTAAACCCTACYTGTCTGGCTTATTTATTTGTTTGACTAATAACTAGTTGTAGTTAATTAGAATGTAGTTGACCTATTTAGGTTAGTTGGTAGGTTTTACGTTTTTACCCTTAACCCGTTAACCAAAGTTAAAAGTTTAAAAA
TARGET=57,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00079 Ps_contig_mira-and-tgicl-ass_franssen_1013
SEQUENCE=GGGTATTGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGTATTTCNCAGTTCAGCTGAAGGAAGGAGCTCGAGNGCACTACAGAAAGAAGTAACTTAACCGCTG
TARGET=56,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9041
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=1.773775
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=ACTTCTTTCTGTAGTGCNCTCG
PRIMER_LEFT=26,22
PRIMER_RIGHT=128,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=58.226
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.50
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.0750
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=103
PRIMER_PAIR_PENALTY_1=1.9041
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=1.773775
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=ACTTCTTTCTGTAGTGCNCTCG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=128,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=58.226
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.50
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.0750
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=104
PRIMER_PAIR_PENALTY_2=2.2904
PRIMER_LEFT_2_PENALTY=0.516652
PRIMER_RIGHT_2_PENALTY=1.773775
PRIMER_LEFT_2_SEQUENCE=ATAGCTCAACAGTGGGAGGATG
PRIMER_RIGHT_2_SEQUENCE=ACTTCTTTCTGTAGTGCNCTCG
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=128,22
PRIMER_LEFT_2_TM=60.517
PRIMER_RIGHT_2_TM=58.226
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.50
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.0750
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=101
PRIMER_PAIR_PENALTY_3=2.5679
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=2.437528
PRIMER_LEFT_3_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_3_SEQUENCE=TTACTTCTTTCTGTAGTGCNCTCG
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=130,24
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=59.562
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.50
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.0750
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=2.5679
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=2.437528
PRIMER_LEFT_4_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_4_SEQUENCE=TTACTTCTTTCTGTAGTGCNCTCG
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=130,24
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.562
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.50
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.0750
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_4=106
=
PRIMER_SEQUENCE_ID=SSR_00080 Ps_contig_mira-and-tgicl-ass_franssen_1031
SEQUENCE=CCTTAATGATTCAGAATTACTTCTATAAAAAAATAAGTATCTGCTGTGTCTTTGGGAATTTTAATCAGTGTGTTTCTGGTGTGATGATCTGATAGCGTTCTGCTTTGATTTTCTTTCTTTCTTATGTAAACTACATTGTGCTGAATAAATGATATTATATGCACATGTATGAATGGCATGTAAGGTATGATATTGAAGAAGTTAACCAAAGTAGGTYAAAAAATTACAAAAG
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00081 Ps_contig_mira-and-tgicl-ass_franssen_1035
SEQUENCE=AAGCAGTGGTACAACGCAGAGTKTTTACTTAAACTTTGTTTGTTAAGGTAAGTAAGTAATATTGTGGTGAGATTAAAGTGGCTCTCACTTTTACACCTGAGAGAAATGAAGAACAGTGGTATCAACGCAGAGGAGAGTGGTGGATGGAAAGAATCAGCTAGTGAATATTGAAGTGAATCAAGGATTTGCATTTACAGATTTCCAAGGTCTAAAATTACTGCTACAAAAAAATCTCTTCAGTCCCTTTAAGAATTTTAATCAATGTGTTTTCTGGTGTGATGATATGATAGTGTTCTGTTTTTACTTTCAACCAATTTGATATATAAACTATATTATCCTTGTTATAACATTGTACGCACTAAAATTTATTCTGATAACAGAACACGTTCAACAAATTTCTGAATGTATAGATATAATCAAATAAACACTGTCAAGACCTTATTCAAATTCGAAAACAGAAACAGTGAAATAAGAGTATATAG
TARGET=48,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00082 Ps_contig_mira-and-tgicl-ass_franssen_1036
SEQUENCE=AAGCAGTGGTTCAACGCAGAGTTTTTACTTAAACTTTGTTGAAGGAAGAAGAATATTGTGGTGAGATTAAAGTGGCTCTCACTTTTACACCTGAGAGAAATGAAGAACAGGGTTACAATGAAGAGGAGAGTGGTGGATGGAAAGAATCAGCTAGTGAATATTGAAGTGAATCAAGGATTTGCATTTACAGATTTCCAAGGTCTAAAATTACTGCTAACAAAAAAATCTTCTTCAGTACCCCTTTAAAGTAAATTTTAATCAATGTGTTTTCTGGTGTGATGATATGATAGTGTTTCTTGTTTTTAACTTTACCAAACCAATTTTAGGTATTAATTAATTAAAAACCTTAATTATTACTTCCTTTAGGTTTAATTAAACCTAATTGGTTACCGGACCTAAACTTTAAAAATTTTAATTTCTTA
TARGET=329,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3275
PRIMER_LEFT_PENALTY=0.361394
PRIMER_RIGHT_PENALTY=1.966130
PRIMER_LEFT_SEQUENCE=AGTGGTGGATGGAAAGAATCAG
PRIMER_RIGHT_SEQUENCE=AAGTTTAGGTCCGGTAACCAAT
PRIMER_LEFT=128,22
PRIMER_RIGHT=402,22
PRIMER_LEFT_TM=60.361
PRIMER_RIGHT_TM=58.034
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=2.4173
PRIMER_LEFT_1_PENALTY=0.451213
PRIMER_RIGHT_1_PENALTY=1.966130
PRIMER_LEFT_1_SEQUENCE=GGTGGATGGAAAGAATCAGCTA
PRIMER_RIGHT_1_SEQUENCE=AAGTTTAGGTCCGGTAACCAAT
PRIMER_LEFT_1=131,22
PRIMER_RIGHT_1=402,22
PRIMER_LEFT_1_TM=60.451
PRIMER_RIGHT_1_TM=58.034
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=2.4768
PRIMER_LEFT_2_PENALTY=0.510631
PRIMER_RIGHT_2_PENALTY=1.966130
PRIMER_LEFT_2_SEQUENCE=ATGAAGAGGAGAGTGGTGGATG
PRIMER_RIGHT_2_SEQUENCE=AAGTTTAGGTCCGGTAACCAAT
PRIMER_LEFT_2=117,22
PRIMER_RIGHT_2=402,22
PRIMER_LEFT_2_TM=60.511
PRIMER_RIGHT_2_TM=58.034
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=2.5098
PRIMER_LEFT_3_PENALTY=0.543622
PRIMER_RIGHT_3_PENALTY=1.966130
PRIMER_LEFT_3_SEQUENCE=ACAATGAAGAGGAGAGTGGTGG
PRIMER_RIGHT_3_SEQUENCE=AAGTTTAGGTCCGGTAACCAAT
PRIMER_LEFT_3=114,22
PRIMER_RIGHT_3=402,22
PRIMER_LEFT_3_TM=60.544
PRIMER_RIGHT_3_TM=58.034
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=2.5232
PRIMER_LEFT_4_PENALTY=0.557021
PRIMER_RIGHT_4_PENALTY=1.966130
PRIMER_LEFT_4_SEQUENCE=GAGAGTGGTGGATGGAAAGAAT
PRIMER_RIGHT_4_SEQUENCE=AAGTTTAGGTCCGGTAACCAAT
PRIMER_LEFT_4=125,22
PRIMER_RIGHT_4=402,22
PRIMER_LEFT_4_TM=59.443
PRIMER_RIGHT_4_TM=58.034
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_00083 Ps_contig_mira-and-tgicl-ass_franssen_1066
SEQUENCE=CCTTTTTTGTGACTATGTTCATCACTATCATCTGGAGACTTGTCCCTTCTGCTTCTTCTGTGCTCCCTCTTTTTCTTCCTCCTCCTCCTTTCACCGGAATCATCCGAATCACTAGCTGCCCTATTAGTCTTAACACTCCTCTTCTTTGCTCTGCCTCTCTTTCTCCTCTTCCGGTCAGAATCTTTGTCTGACTCATCATCATCTGAGTCCTCCTTCTTTCTATAAGAACCTCTCTTCCCACTAACTTTCTTCCCTGACCTTTCAGCAATAKYCMTCTMRRTTTTTCAGCCAATGGATTCGCCAAAGGTGGAACTTTAGCAAGAAACTAAAGAAGCACCACCACCATTAGTTTCTACTTTAGAACTCATTCTGTCGGAAGTTGAAGTTGAATAATTGAAGAAACGGTGAAGAGGGAGATTAGAAAGGAAAAGGAGAATCTGGATTTGGAAACATACGTGAAGATATTAGAAGGAATGGAATTGCTAGGCCACGAAAGAGTATCAAAAGATATGATGCTAAATGATAGCTCAAGTGAGAGTGAGGCACAGAAAGAGAGAAAGGGGTGTGTGTGGAGTTTGTGAATGATCAAAAGAAGTGGCSMMRATNTCTTAG
TARGET=77,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00084 Ps_contig_mira-and-tgicl-ass_franssen_1071
SEQUENCE=ATGTTAACTAAATTTTATTTTCAATAAGATAAGAAACAGATGCATTGATTAAGAGAGACGACACTCTACAATAATAATAATAACACAGTTAGAAACTCGACACAGCATACTATAGTACAAATATAAGTAAAGTTATTGTTTTCGTTTTTATTCATGACTATACTCTTCTCTAATTAGTATTACAATGACTTATTAATTATTA
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00085 Ps_contig_mira-and-tgicl-ass_franssen_1093
SEQUENCE=GAATCATTAATCACCACCTTTATTTTATTCATTTTCGCATCGAAACTCACATGGCCAATTATAGTATTTAATTATTTATAATTATTATATTTATTKACGTACTATATTTATTTATTTTCGTGGACTCTATTCTCTTGACCATCCCCATTGTTTTTCTTCATCGTTCTTCTTTCTATTCCAACAATTTTCCGATTTCGAACGATATCATGGAAGAAGTTAACGTTGTTGAAACTAAGGATGGAACCGTTTCGGTAGCTACTGCGTTTGCTGGTCACCAGGAAGCTGTGCAGGCTAGAGACCACAAATTTTTAAGAAAAGCAGTTGAAGAAGCATATAAAGGGGTAGATTGTGGAGATGGAGGCCCTTTTGGTGCTGTTATTGTTCACAACGATGAAGTAGTTGCTAGTTGTCACAACATGGTTCTGAATTACACCGATCCAACTGCTCATGCAGAGGTCACAGCAATCAGAGAGGCTTGTAAGAAGCTTAAGCAGATAGAACTTTCAGATTGTGAAATATATGCTTCTTGTGAACCCTGCCCCATGTGCTTTGGTGCAATCCACCTCTCGCGGATTAAGAGATTGGTTTATGGAGCAAAGGCAGAGGCAGCAATCGCTATTGGGTTTGACGATTTTATTGCAGATGCATTGCGAGGTACTGGATTCTACCAGAAAGCAACGTTGGAAATTAAAAGAGCTGATGGTAATGAGGCCATGATTGCAAGAAGGTTAGGTTTTWGAAGAAAAACAAAGGACTAAAAACTTACCTAAAATGTTAAGTTTGAATTATTACCTAATTTCTTTTACACAACATTAAGTGAACTAATCTACGACCGTAAACTTAAACCGTTTACGTTTAACGTAACGTTAAACGGGGTACCGGGGTTAACGTTTTAAGGTTTAA
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00086 Ps_contig_mira-and-tgicl-ass_franssen_1119
SEQUENCE=TACTTGATTTCATTTGGTGGGCTTCTGATGATGATGAAGGGAGATCACTCTCATTTCAACTAAGTTTGAACTTGATACAAAGGTTTGTTTCTTCTGAATTAAGGAAAGTTATAGGAAAGAATTACTAAAAGGTTTAAAAAACCGTTTCTTAACATCTAATTGTTTAGGGTAAGGTTTTCGTTTACCGTTTAGTAATACTAA
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5485
PRIMER_LEFT_PENALTY=1.065617
PRIMER_RIGHT_PENALTY=0.482886
PRIMER_LEFT_SEQUENCE=TACTTGATTTCATTTGGTGGGC
PRIMER_RIGHT_SEQUENCE=ACGGTAAACGAAAACCTTACCC
PRIMER_LEFT=0,22
PRIMER_RIGHT=187,22
PRIMER_LEFT_TM=61.066
PRIMER_RIGHT_TM=60.483
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=1.6594
PRIMER_LEFT_1_PENALTY=1.065617
PRIMER_RIGHT_1_PENALTY=0.593822
PRIMER_LEFT_1_SEQUENCE=TACTTGATTTCATTTGGTGGGC
PRIMER_RIGHT_1_SEQUENCE=CGGTAAACGAAAACCTTACCCT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=186,22
PRIMER_LEFT_1_TM=61.066
PRIMER_RIGHT_1_TM=60.594
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=1.7962
PRIMER_LEFT_2_PENALTY=1.313269
PRIMER_RIGHT_2_PENALTY=0.482886
PRIMER_LEFT_2_SEQUENCE=GATTTCATTTGGTGGGCTTCT
PRIMER_RIGHT_2_SEQUENCE=ACGGTAAACGAAAACCTTACCC
PRIMER_LEFT_2=5,21
PRIMER_RIGHT_2=187,22
PRIMER_LEFT_2_TM=60.313
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=1.9071
PRIMER_LEFT_3_PENALTY=1.313269
PRIMER_RIGHT_3_PENALTY=0.593822
PRIMER_LEFT_3_SEQUENCE=GATTTCATTTGGTGGGCTTCT
PRIMER_RIGHT_3_SEQUENCE=CGGTAAACGAAAACCTTACCCT
PRIMER_LEFT_3=5,21
PRIMER_RIGHT_3=186,22
PRIMER_LEFT_3_TM=60.313
PRIMER_RIGHT_3_TM=60.594
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=2.3059
PRIMER_LEFT_4_PENALTY=1.065617
PRIMER_RIGHT_4_PENALTY=1.240245
PRIMER_LEFT_4_SEQUENCE=TACTTGATTTCATTTGGTGGGC
PRIMER_RIGHT_4_SEQUENCE=CGGTAAACGAAAACCTTACCC
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=186,21
PRIMER_LEFT_4_TM=61.066
PRIMER_RIGHT_4_TM=59.760
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00087 Ps_contig_mira-and-tgicl-ass_franssen_1120
SEQUENCE=AAGCAGTGGTATCAACGCAGAGTTACTTGTATTTCATTTGGTGGGCTTCTGATGATGATGAAGGGAGATCACTCTCATTTCAACAAGTTTGAACTTGATCAAAGGTTGTTTCTTCTGATTAGGAAAGTATGAAGATCAAAGTTTAAAAACGTTTCTTACATCAATTGTTTGGAAGGTTTTGTTTACCGTTTGTAATACTAATGCGGATCTTCGCGATCCAATGATTAGTTTAAAAACAATGTTGTACCTTGAAGTTAACTTTTGGCAAGAAATGATCAAACA
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3281
PRIMER_LEFT_PENALTY=0.115769
PRIMER_RIGHT_PENALTY=0.212376
PRIMER_LEFT_SEQUENCE=ACTTGTATTTCATTTGGTGGGC
PRIMER_RIGHT_SEQUENCE=CAAACGGTAAACAAAACCTTCC
PRIMER_LEFT=24,22
PRIMER_RIGHT=191,22
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=59.788
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.3281
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.212376
PRIMER_LEFT_1_SEQUENCE=ACTTGTATTTCATTTGGTGGGC
PRIMER_RIGHT_1_SEQUENCE=CGGTAAACAAAACCTTCCAAAC
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=187,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=59.788
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.4491
PRIMER_LEFT_2_PENALTY=0.236697
PRIMER_RIGHT_2_PENALTY=0.212376
PRIMER_LEFT_2_SEQUENCE=CTTGTATTTCATTTGGTGGGCT
PRIMER_RIGHT_2_SEQUENCE=CAAACGGTAAACAAAACCTTCC
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=191,22
PRIMER_LEFT_2_TM=60.237
PRIMER_RIGHT_2_TM=59.788
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.5747
PRIMER_LEFT_3_PENALTY=0.115769
PRIMER_RIGHT_3_PENALTY=0.458951
PRIMER_LEFT_3_SEQUENCE=ACTTGTATTTCATTTGGTGGGC
PRIMER_RIGHT_3_SEQUENCE=AACTAATCATTGGATCGCGAAG
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=229,22
PRIMER_LEFT_3_TM=60.116
PRIMER_RIGHT_3_TM=60.459
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.6956
PRIMER_LEFT_4_PENALTY=0.236697
PRIMER_RIGHT_4_PENALTY=0.458951
PRIMER_LEFT_4_SEQUENCE=CTTGTATTTCATTTGGTGGGCT
PRIMER_RIGHT_4_SEQUENCE=AACTAATCATTGGATCGCGAAG
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=229,22
PRIMER_LEFT_4_TM=60.237
PRIMER_RIGHT_4_TM=60.459
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_00088 Ps_contig_mira-and-tgicl-ass_franssen_1122
SEQUENCE=CGTTTCTTTAATTTTGTATTTTTTGGCAGAGAGTTACTGATTTCATTTGGTGGGCTTCTGATGATGATGAAGGGAGATCACTCTCATTTCAACAAGTTTGAACTTGATCAAAGGTTGTTTCTTCTGATTAGGAAAGTATGAAGATACAAAGTTAAAAACGTTTCTTACATCAATTGGTTTAGGTAAGGTTTTGTTTACCGTTTGTAATACTAATGCGGAGTTCTTCGCGATCCAATGAATTAAGTTTTAAAAAMMAATTAGTTGTACCTTGAAGTACTCTTGGACAAGAAATGAA
TARGET=59,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00089 Ps_contig_mira-and-tgicl-ass_franssen_1125
SEQUENCE=TTAAGTCTTAGTTGGTTATTCAACGCAGAGTTACTTGTATTTCATTTGGTGGGCTTCTGATGATGATGAAGGGAGATCACTCTCATTTTCAACAAGTTTGAACTTGATACAAAAGGTTGTTTTCTTCTGATTAGGAAAGTATGAAGATCAAAGTTAAAAACGTTTCTTACATCAATTGTTTGGAAGGTTTWGTTTAACCGTTTAGTAAATACTAATGCGGAGTTCTTCGCGATCCAATGATTAGTTTTAAAAACAAATGTTGTACCTGAAGTTAACTTTTAGACAAGAAATG
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00090 Ps_contig_mira-and-tgicl-ass_franssen_1134
SEQUENCE=TTCCTTCAGCTTGCGTCGGAGACANGGTGATGGCCACCGTGAAAGAAAGGGGAAAAAGGACCCAGAATCTGTAGGGAAAAAAGGTTTTAGCCTGCTGTCATCGTTCGTCAACGTAAGCCATGGCGCCGAAAGGACGGTGTCTTCATGTACTTTGTAAGATAATGCTGGTGTCATTGTGAATCCTAAGGGTGAAATGAAAGGTTCTGCCATCACTGGTCCAATCGGTAAGGAGTGTGCTAGATACTAGTCGGACCGAAGGTATTGACTAAGTCGTACCTAAACCTAAGTCCGACCTTAAGTTTAGTTAAGTAGGTAGGTAGTAACTTACGTTTAACGGTTTAAACTACCGACCCTAGTTTGTTT
TARGET=309,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6718
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.671790
PRIMER_LEFT_SEQUENCE=GCTGGTGTCATTGTGAATCCTA
PRIMER_RIGHT_SEQUENCE=GGGTCGGTAGTTTAAACCGTTA
PRIMER_LEFT=163,22
PRIMER_RIGHT=352,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=59.328
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=0.6761
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.671790
PRIMER_LEFT_1_SEQUENCE=AATGAAAGGTTCTGCCATCACT
PRIMER_RIGHT_1_SEQUENCE=GGGTCGGTAGTTTAAACCGTTA
PRIMER_LEFT_1=192,22
PRIMER_RIGHT_1=352,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.328
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=161
PRIMER_PAIR_PENALTY_2=0.6865
PRIMER_LEFT_2_PENALTY=0.014720
PRIMER_RIGHT_2_PENALTY=0.671790
PRIMER_LEFT_2_SEQUENCE=CGGACCGAAGGTATTGACTAAG
PRIMER_RIGHT_2_SEQUENCE=GGGTCGGTAGTTTAAACCGTTA
PRIMER_LEFT_2=248,22
PRIMER_RIGHT_2=352,22
PRIMER_LEFT_2_TM=60.015
PRIMER_RIGHT_2_TM=59.328
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=0.8061
PRIMER_LEFT_3_PENALTY=0.134318
PRIMER_RIGHT_3_PENALTY=0.671790
PRIMER_LEFT_3_SEQUENCE=AATGCTGGTGTCATTGTGAATC
PRIMER_RIGHT_3_SEQUENCE=GGGTCGGTAGTTTAAACCGTTA
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=352,22
PRIMER_LEFT_3_TM=59.866
PRIMER_RIGHT_3_TM=59.328
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.8827
PRIMER_LEFT_4_PENALTY=0.210886
PRIMER_RIGHT_4_PENALTY=0.671790
PRIMER_LEFT_4_SEQUENCE=AAGGTTTTAGCCTGCTGTCATC
PRIMER_RIGHT_4_SEQUENCE=GGGTCGGTAGTTTAAACCGTTA
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=352,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=59.328
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_00091 Ps_contig_mira-and-tgicl-ass_franssen_1156
SEQUENCE=TTGCCTATTCTAATACTGGAAAACAAAACATCAAAGGTCTTTCCAACCTAACTGGCTATGTTAAGGGTAATGCTAATGGAGTTATCAACCTTGGTTCCTTGATAGCCTCTGCAAATGCTCAGAGACAAGCATGAATGAATGAACTCCATAGAAGACTAAATTAATTGTTAATTACGTTCGGTTGGGGTAGGGACGGACCACGTACCTTAACTAAATTTAAACTTTAAAACTTTAAAACCTTAAAAACTTAAAAATTTAAATTTATTTTACTTTACGTTTTAGTTCGGTT
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1677
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=0.946688
PRIMER_LEFT_SEQUENCE=TGGAGTTATCAACCTTGGTTCC
PRIMER_RIGHT_SEQUENCE=CCCAACCGAACGTAATTAACAA
PRIMER_LEFT=76,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=60.947
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=1.3067
PRIMER_LEFT_1_PENALTY=0.360050
PRIMER_RIGHT_1_PENALTY=0.946688
PRIMER_LEFT_1_SEQUENCE=AACCTTGGTTCCTTGATAGCCT
PRIMER_RIGHT_1_SEQUENCE=CCCAACCGAACGTAATTAACAA
PRIMER_LEFT_1=86,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=60.360
PRIMER_RIGHT_1_TM=60.947
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=1.4295
PRIMER_LEFT_2_PENALTY=0.482784
PRIMER_RIGHT_2_PENALTY=0.946688
PRIMER_LEFT_2_SEQUENCE=TCAACCTTGGTTCCTTGATAGC
PRIMER_RIGHT_2_SEQUENCE=CCCAACCGAACGTAATTAACAA
PRIMER_LEFT_2=84,22
PRIMER_RIGHT_2=185,22
PRIMER_LEFT_2_TM=60.483
PRIMER_RIGHT_2_TM=60.947
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=102
PRIMER_PAIR_PENALTY_3=1.4766
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=1.255615
PRIMER_LEFT_3_SEQUENCE=TGGAGTTATCAACCTTGGTTCC
PRIMER_RIGHT_3_SEQUENCE=CCCAACCGAACGTAATTAACA
PRIMER_LEFT_3=76,22
PRIMER_RIGHT_3=185,21
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.744
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=110
PRIMER_PAIR_PENALTY_4=1.5759
PRIMER_LEFT_4_PENALTY=0.629225
PRIMER_RIGHT_4_PENALTY=0.946688
PRIMER_LEFT_4_SEQUENCE=AACATCAAAGGTCTTTCCAACC
PRIMER_RIGHT_4_SEQUENCE=CCCAACCGAACGTAATTAACAA
PRIMER_LEFT_4=26,22
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=59.371
PRIMER_RIGHT_4_TM=60.947
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_00092 Ps_contig_mira-and-tgicl-ass_franssen_1157
SEQUENCE=TTGCCTATTCTAATACTGGAAAACAAAACATCAAAGGTCTTTCCAACCTAACTGGCTATGTTAAGGGTAATGCTAATGGAGTTATCAACCTTGGTTCCTTGATAGCCTCTGCAAATGAACTCAGAGACAAGCAYGAATGAATGAATCMATGAAGCAATAATGTATATCTGGGTGGAGGCAGCTANAATATAATTACAATAAATAATNTTATCTTATGTT
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00093 Ps_contig_mira-and-tgicl-ass_franssen_1159
SEQUENCE=TTGTCCTTATTCTAATACTGGAAAACAAAACATCAAAGGTCTTTCCAACCTAACTGGCTATGTTAAGGGTAATGCTAATGGAGTTATCAACCTTGGTTCCTTGATAGCCTCTGCAAATGCTCAGAGACAAGCATGAATGAATGAACTCCAATAGAAAGACTAAATTAAGTTGTTAATAGTCGGTGGGTAGGAACGAACCGACGTACCTTAACTAAAATTTAAACTTTAAAACTTTAAAACCTTAAAAACTTAAAATTAAATTATTTATTAC
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2874
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=TGGAGTTATCAACCTTGGTTCC
PRIMER_RIGHT_SEQUENCE=GTTAAGGTACGTCGGTTCGTTC
PRIMER_LEFT=78,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.2904
PRIMER_LEFT_1_PENALTY=0.224014
PRIMER_RIGHT_1_PENALTY=0.066394
PRIMER_LEFT_1_SEQUENCE=TTCCTTGATAGCCTCTGCAAAT
PRIMER_RIGHT_1_SEQUENCE=GTTAAGGTACGTCGGTTCGTTC
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=60.224
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=116
PRIMER_PAIR_PENALTY_2=0.3209
PRIMER_LEFT_2_PENALTY=0.254467
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=CTTGGTTCCTTGATAGCCTCTG
PRIMER_RIGHT_2_SEQUENCE=GTTAAGGTACGTCGGTTCGTTC
PRIMER_LEFT_2=91,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=60.254
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=121
PRIMER_PAIR_PENALTY_3=0.4264
PRIMER_LEFT_3_PENALTY=0.360050
PRIMER_RIGHT_3_PENALTY=0.066394
PRIMER_LEFT_3_SEQUENCE=AACCTTGGTTCCTTGATAGCCT
PRIMER_RIGHT_3_SEQUENCE=GTTAAGGTACGTCGGTTCGTTC
PRIMER_LEFT_3=88,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=60.360
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=0.4702
PRIMER_LEFT_4_PENALTY=0.220992
PRIMER_RIGHT_4_PENALTY=0.249232
PRIMER_LEFT_4_SEQUENCE=TGGAGTTATCAACCTTGGTTCC
PRIMER_RIGHT_4_SEQUENCE=CGTTCCTACCCACCGACTATTA
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=194,22
PRIMER_LEFT_4_TM=60.221
PRIMER_RIGHT_4_TM=60.249
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=117
=
PRIMER_SEQUENCE_ID=SSR_00094 Ps_contig_mira-and-tgicl-ass_franssen_1160
SEQUENCE=AGTAATTTAACCAACTATTTTATTGTAGTATAATGGCTATGATGGTGTAAGACACACATTATAATAGGATGAGGCAACATAAGATAATATTTATTTAGTTAGTAATTATATTGAGCTGCCTCCACCAGATATACATTATTGCTTCAGTTGGRTTCATTCATTCATGTCTTGTCTCTGAGCTATTTGCAGGNGGTCTATACGAAACGGGAAACCGTAAGGGTTGTAAA
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00095 Ps_contig_mira-and-tgicl-ass_franssen_1161
SEQUENCE=GATAATTWAACCAACATTTTATTGTAGTATAATGGCTATGATGGTGTAAGACACACATTATAATAGGATGAGGCAACAKAWRATATTTATTTAGTTAGTAATTATATTGAGCTGCCTCCACCAGATATACATTATTGCTTCATGGATTCATTCATTCATGCTTGTCTCTGAGCTATTTGCAGAGGCTATCAAGGAACCAGGTT
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00096 Ps_contig_mira-and-tgicl-ass_franssen_1189
SEQUENCE=TGGTATCAACGCAGAGTAGTCGGGKGACACAACACGTTAATTTTATCTTCACTCTTCTTCAGATCTCAAATTTCATTTCTCGCTCACATCGATTTCTCCTCCTCCTCCATGGCCGCTATTACTTCCATCAAGGCTCGTCAGATCTTCGACAGCCGTGGCAATCCAACCGTCGAGGTTGATATAACTGTCTCCGATGGCACTTTCGCTAGAGCCGCTGTTCCTAGCGGTGCATCCACTGGTATTTACGAGGCTCTTGAGTTGAGAGATGGAGGATCTGACTATCTTGGAAAAGGTGTATCAAAGGCTGTTGACAATGTGAACACCATCATTGCCCCAGCATTGATCGGCAAGGACCCAACTAAGCAGACTGAGATTGACAACTTCATGGTTCAGCAGCTCGATGGAACTGTTAATGAGTGGGGTTGGTGTAAACAGAAGCTTGGGGCAAATGCCATATTGGCAGTGTCTCTTGCAGTCTGCAAAGCTGGTGCTGGAGTCCTAAAAACCCCTCTTTACAAGCATATTGCAAACATTGCAGGCAACAAGAATTTGGTTTTGCCTGTTCCAGCTTTCAATGTCATTAATGGTGGATCACATGCAGGAAACAAGCTTGCCATGCAGGAGTTTATGGTTCTTCCTGTGGGAGCCTCCTCTTTCAAGGAAGCCATGAAGATGGGTGTGGAAGTCTATCACCATTTGAAGGCTGTGATTAAGAAGAAATATGGTCAAGATGCAATCAATGTTGGTGACGAAGGTGGTTTTGCCCCTAACATTCAGGAGAACAAAGAAGGTTTGGAGTTGCTAAAATCTGCCATTGACAAAGCCGGATACACTGGCAAAGTTGTCATTGGAATGGATGTTGCAGCTTCCGAGTTCTACAAGGAAGACAAGTCATATGATTTGAACTTCAAGGAAGATAACAACGACGGCTCACAGAAGATCTCAGGAGAGGCTTTGAAAGATCTCTACAAGTCATTTGTGTCAGAGTACCCAATTGTTTCAATTGAGGATCCTTTTGACCAGGATGACTGGGAGCACTATGCCAAGATGACTGGTGAGATTGGAACCAATGTGCAAATTGTTGGTGATGATCTCTTGGTTACCAACCCCAAGAGGGTTCAGAAGGCAATTGACTCAAAAGCATGCAATGCTCTTCTTCTCAAGGTGAACCAAATTGGATCAGTGACTGAAAGTATTGAAGCCGTGAGGATGTCCAAAAAGGCTGGATGGGGTGTCATGACCAGCCACCGAAGTGGAGAGACTGAGGATACCTTCATTGCTGATCTTGCTGTTGGTTTGTCAACGGGTCAAATTAAGACCGGAGCTCCATGCAGGTCAGAGCGTCTAGCTAAGTATAACCAGCTCTTGAGAATTGAGGAGGAGCTCGGTGCTGAAGCAGTGTATGCTGGAGTTAACTTCCGTACCTYCYGTGWYGACCCCTTAAAGTTAAAAAAGGGTTTTTAGGAAAAAAACGGGGGGGTAAAAAAAAACCCCCTAAAGGGTAAACGTTTAAACCGTTAAACCCCCCGTAAAAACCCGGTTTTAGGGTTTAAAGGGTTTTTTTTAACCGGG
TARGET=96,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00097 Ps_contig_mira-and-tgicl-ass_franssen_1200
SEQUENCE=CGGGTTTTCCTAGTCCACCCCCTTCCCTTCCTTCCTTTTGTTCGTTAACCAACTGGTGGAGAGTCAGTGTGTTGAACGATGCTCTTAAGAGTATGTACAATGCTGAGAAAAGAGGAAAGCGCCAAGTCATGATAAGGCCATCATCCAAAGTTATCATCAAGTTCCTTATTGTTATGCAGAAGCATGGATATACTGGAGAATTTGAGTATGTTGATGATCACCGAGCTGGAAAGATTGTTGTTGAATTGAATGGTAGGTTAAACAAATGTGGGGTTATTAGTCCTCGTTTTGATGTTGGTGTCAAGGAGATTGAAGGTTGGACTGCCAGACTTCTCCCTTCTAGACAGTTTGGTTACATTGTTTTGACCACTTCTGCTGGCATTATGGATCACGAAGAGGCTAGGAGGAAGAATGTTGGTGGTAAGGTCCTTGGCTTCTTCTACTGAGAGTTTTTGTGATTTCAACCTGATGTAATGGAGGATCCTAGTTCCCTAGTAATTGAAGGAGCTGTTTTTGTGATGTGATTTAATTTTATTTTGTGAAACCTAGTTCAGTATAATTTATCTGATTTCTTCTATAAATTTTTGATTGTACCGGAATGAATGGCATACTTGTTTTGAATGGTATAGAATTTTATTATAATGTTGGAGACAYMAAGAGTTTCAATTGGTATGGTCCAGTTGTCAATATTTGGAAAGTATCAACTAGTTCTCAAGTTAGATTAAACAACTCTGAAAAATTTCATGTTTAAC
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00098 Ps_contig_mira-and-tgicl-ass_franssen_1206
SEQUENCE=GAATAATAATAATAATTGGAACACAAAAAACTACCATCACCACTAAGATTCCTCACATATCTATTCAGACCTCTTAGCATTGCCRWCRSSGAAATCACATACTAGAATGGCAACAACAACCTTTGCAACCGTCGTAGGATGGGTGGTGCATCACGTCTCACCTCAGGATTTGAACGAAACCACAAGTAACTGCTAGGAATTCCTTGAGGACAAGCTAGTGGGCGGTAAGGTAAAG
TARGET=2,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00099 Ps_contig_mira-and-tgicl-ass_franssen_1210
SEQUENCE=GAAGTATTCAATTTTTTCATAGAAGTCAATAATAATAATAATAATAATTGGAACACAAAAACTACCATCACCACTAAGATTCCTCACATATCTATTCAGACCTCTTAGCATTGCCAACACCGAAATCACATACTAGAATGGCAACAACACTTGCACCGTGTAGGATGGGTGGTGCATCACGTCCTCCACCTCGAGGTAGTTTACGGGAAACCA
TARGET=28,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00100 Ps_contig_mira-and-tgicl-ass_franssen_1216
SEQUENCE=CCTACCTTACCCCTTCCCCGTTGGTTGGTCCGTTCCGTTCCGTACCTTACCCCTTCCCCGTTTGGTTCGGTCGGTTCGTTCCTACGACGTTCGTTAGTTAGGGTTCGGATAANAANACTCCTACTTAGTTTGTCNCCTCCTCTTTGTGAACCCAATGCAAAACATCCTAAGAGTGTAATTATCGGTGGTTTTCACATCAACATGGGCTTCAATAAGAGTTTGCCATTTGCGAACCAATGACCTGAGCTTGTCAGTTGTGAAATCCATACCCCAGAAGTTGGTCAAGAGGTTTTTCCCCTGAACATCTTCAGCCCTCAGTCTAATCTTCCTGAAAGCGTTGTCTTCATCTCCTTGAAGNTCAGCCAATGACTACCTACAAACACACGTATTGGTTTAGACGTCCAGTCGGGAANCAATTCGTTCGGGTACCCTGGA
TARGET=29,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.4806
PRIMER_LEFT_PENALTY=3.425403
PRIMER_RIGHT_PENALTY=0.055230
PRIMER_LEFT_SEQUENCE=CTACCTTACCCCTTCCCCGT
PRIMER_RIGHT_SEQUENCE=CGAACCCTAACTAACGAACGTC
PRIMER_LEFT=1,20
PRIMER_RIGHT=106,22
PRIMER_LEFT_TM=61.425
PRIMER_RIGHT_TM=60.055
PRIMER_LEFT_GC_PERCENT=60.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.1000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=3.5068
PRIMER_LEFT_1_PENALTY=3.425403
PRIMER_RIGHT_1_PENALTY=0.081398
PRIMER_LEFT_1_SEQUENCE=CTACCTTACCCCTTCCCCGT
PRIMER_RIGHT_1_SEQUENCE=CACAAAGAGGAGGNGACAAACT
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=147,22
PRIMER_LEFT_1_TM=61.425
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=3.5349
PRIMER_LEFT_2_PENALTY=3.425403
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=CTACCTTACCCCTTCCCCGT
PRIMER_RIGHT_2_SEQUENCE=CTGGGGTATGGATTTCACAACT
PRIMER_LEFT_2=1,20
PRIMER_RIGHT_2=273,22
PRIMER_LEFT_2_TM=61.425
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=3.5614
PRIMER_LEFT_3_PENALTY=3.425403
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CTACCTTACCCCTTCCCCGT
PRIMER_RIGHT_3_SEQUENCE=TCTTGACCAACTTCTGGGGTAT
PRIMER_LEFT_3=1,20
PRIMER_RIGHT_3=286,22
PRIMER_LEFT_3_TM=61.425
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.1000
PRIMER_RIGHT_3_END_STABILITY=6.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=3.5965
PRIMER_LEFT_4_PENALTY=3.541267
PRIMER_RIGHT_4_PENALTY=0.055230
PRIMER_LEFT_4_SEQUENCE=CTACCTTACCCCTTCCCCGTT
PRIMER_RIGHT_4_SEQUENCE=CGAACCCTAACTAACGAACGTC
PRIMER_LEFT_4=1,21
PRIMER_RIGHT_4=106,22
PRIMER_LEFT_4_TM=62.541
PRIMER_RIGHT_4_TM=60.055
PRIMER_LEFT_4_GC_PERCENT=57.143
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=106
=
PRIMER_SEQUENCE_ID=SSR_00101 Ps_contig_mira-and-tgicl-ass_franssen_1217
SEQUENCE=TTGTTGGGGGGCGTAAGTTCGTTGCTAGTTTTTGGGCCTTATTGTATTGTTATTTATTTATTCTACAAGATTCTGAAATAGTTATCATATGAAGTATCGGTCCTATTTATGCATTGAGGTTGCAGACATTTGTTTATCCAATCTACAGTTTGTCCACAATTAGAAGTATTCCGTCAGCATTATTGAATTTATGACTTCATTAGTCTAGATGAATGGGATCAGCTGTTAATTCTGTAGTGGGGTAAGTTGGTGATTTTGATTGGGATCAGGGATTCATTTTTTAAGGGTCAGTTGAAAATAAAATTGTTCTTTTCGATTWCCACCGCTTTTTCTAAAGGGGCTTTGCATTGACTTTGTAAATTACTGCCGGAAAATGTGTTTTTTGTTGCATCATCAAACGGTTTTAAAACTT
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00102 Ps_contig_mira-and-tgicl-ass_franssen_1223
SEQUENCE=AAACTTTTTAAAATTTAAGTTACTTGCAACCTCAATGCATAAATAGGACCGATACTTCATATGATACATTTCAGAATCTTGTAGGATAAATAAATAACAATACAATAGGCCAAAACTGCACGAATTCGCCCCACAGACAACCCTATATTCCTCACGCAATATGGTAAAGAAACAAAAMSAGRRGGAATGACCGACGCGAAGCTCCAGCCAACAAATGGACCCTAAACCTTTAAACCCCCCCCGTTTTAAGGGACCTACCAAACTCCGGACCAACCC
TARGET=86,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00103 Ps_contig_mira-and-tgicl-ass_franssen_1224
SEQUENCE=CAATTGTGGTTGGAAAGGGAACTTTCTGTAGAATGTGTATATTTAATAGAGTGTTCATGGTGTGATTGAGTCCTAAAGGGGGTTATGCATTTGTTGGCTAGGAGCTTCGCGTGTCATTCCTCTGTTTTGTTCTTACCATATTGCGTGAGAATATAGGGTTGTCTGTGGGGCAATTCGTGCAGTTTTGGCCTATTGTATTGTTATTTATTTATCCTACAAGATTCTGAAATGTAT
TARGET=201,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00104 Ps_contig_mira-and-tgicl-ass_franssen_1225
SEQUENCE=AAGCAGTGGTATCAACGCAGAGTCAATTGTGATTGGAAAGGGAACTTTCTGTAGAATGTGTATATTTATGAGTGTTCATGGTGCGATTGAGTCCTAAAGGGGGTTATGCATTTGTGGCTAGGAGCTTCGCGTGTCATTCCTCTTGTTTTGTTCTTACCATATTGCGTGAGAATGTAGGGTTGTCTGKKGGGCAATTCGTGCTAGTTTTCGGCCTTATTGTATTGTTATTTATTTATCCTACAAGATTCTGAAAT
TARGET=225,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00105 Ps_contig_mira-and-tgicl-ass_franssen_1227
SEQUENCE=CYTAAGTTTGTTGTATTTGGTAAAGGGTAACTTTTCTTGTAGAATGTGTATATTTATGAGTGTTCATGGTGCGATTGAGTCCTAAAGGGGTTATGCATTTGTGGCTRGGAGCTTCGCGTGTCATTCCTCTTGTTTTGTTCTTACCATATTGCGTGAGAATAGTAGGGTTGTCTGGTGGGGCTAATTTCGGTGCAGTTTTGGCCTNTTGTATTTGTTTATTTATTTATCCTACAAGG
TARGET=215,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00106 Ps_contig_mira-and-tgicl-ass_franssen_1253
SEQUENCE=GTTCGGGTCTTTCTTTCTTCCTAAGTCTACCCTTTCACTCCGAACAACCGATTCCAATGGCTTCAACTTCGATAATCTCAATGGCTGTGCCGGTAACCTGTGCCACCACCAAGAAGATTGAGTCACCGATCTCTCATGCATTCTTCAAGGCACCACNGGAKAKGTGAGCATCATCAAGTACTACGGAAGGTTTCAAGTTGAAACGCATCGCTGAAGGACGAAGTTGTGAACG
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00107 Ps_contig_mira-and-tgicl-ass_franssen_1262
SEQUENCE=TCAACAACAACAACAGCAGCCTCAACATCAACAGCAACAGCAGCCTCAACATCAACAACAGCAACAGCAGCCTCAACAGCAGCAGCTTCAACAACAGCTTCAACAACAGCAGCAGCAAGCAGCAGCTAGTTTATACAGTGGGGGACACTACATTCATCATAACCCTGCTAATTCCTGCATTACTATCCTAGTTATTACTCCTTCACA
TARGET=2,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00108 Ps_contig_mira-and-tgicl-ass_franssen_1267
SEQUENCE=GTAGTAGGGTTTGGAGGGTTTTGGGGTCTACTAGAAGACATTGTTGTGGCAGAATCCCCTATATTGGCCTGCTGCACAGGAAGATTGTAAGCTTGTGGCTGTCTTGGAACATAGTAAACCTGATGATGGGGCTGTTGCTGTGAAGGATATACAGGATAGTATGCAGGAATTGCAGGGTTATGATGAATGTAGTCGTCCCCCATAGTATAAACTGCTGCTGCTGCTGCTGCTGCTGTTGTTGAAGCTGTTGTTGAGCTGCTGCTGTTGAGGCTGCTTGTGCTTGTTGTTGTAGTTTGGGTTTAGGGACGGGTCCTTGGTCCGTTTGGTTTCGGTCGTTTGGTTGTAGTTTGGGTTAGGGACGGGTTCCGTTGGTTCGTTTTGGTTTTTGTTTTTACGTTTTTAACCAAAAC
TARGET=212,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2790
PRIMER_LEFT_PENALTY=0.143555
PRIMER_RIGHT_PENALTY=0.135429
PRIMER_LEFT_SEQUENCE=TAGTAAACCTGATGATGGGGCT
PRIMER_RIGHT_SEQUENCE=CCCTAACCCAAACTACAACCAA
PRIMER_LEFT=111,22
PRIMER_RIGHT=357,22
PRIMER_LEFT_TM=59.856
PRIMER_RIGHT_TM=60.135
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=247
PRIMER_PAIR_PENALTY_1=0.3035
PRIMER_LEFT_1_PENALTY=0.168114
PRIMER_RIGHT_1_PENALTY=0.135429
PRIMER_LEFT_1_SEQUENCE=CTTGTGGCTGTCTTGGAACATA
PRIMER_RIGHT_1_SEQUENCE=CCCTAACCCAAACTACAACCAA
PRIMER_LEFT_1=91,22
PRIMER_RIGHT_1=357,22
PRIMER_LEFT_1_TM=60.168
PRIMER_RIGHT_1_TM=60.135
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.3286
PRIMER_LEFT_2_PENALTY=0.143555
PRIMER_RIGHT_2_PENALTY=0.185055
PRIMER_LEFT_2_SEQUENCE=TAGTAAACCTGATGATGGGGCT
PRIMER_RIGHT_2_SEQUENCE=ACCCAAACTACAACCAAACGAC
PRIMER_LEFT_2=111,22
PRIMER_RIGHT_2=352,22
PRIMER_LEFT_2_TM=59.856
PRIMER_RIGHT_2_TM=60.185
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.3449
PRIMER_LEFT_3_PENALTY=0.143555
PRIMER_RIGHT_3_PENALTY=0.201297
PRIMER_LEFT_3_SEQUENCE=TAGTAAACCTGATGATGGGGCT
PRIMER_RIGHT_3_SEQUENCE=CGTCCCTAACCCAAACTACAAC
PRIMER_LEFT_3=111,22
PRIMER_RIGHT_3=360,22
PRIMER_LEFT_3_TM=59.856
PRIMER_RIGHT_3_TM=59.799
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.3463
PRIMER_LEFT_4_PENALTY=0.210886
PRIMER_RIGHT_4_PENALTY=0.135429
PRIMER_LEFT_4_SEQUENCE=AGGAAGATTGTAAGCTTGTGGC
PRIMER_RIGHT_4_SEQUENCE=CCCTAACCCAAACTACAACCAA
PRIMER_LEFT_4=77,22
PRIMER_RIGHT_4=357,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=60.135
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_00109 Ps_contig_mira-and-tgicl-ass_franssen_1268
SEQUENCE=TTTGAACTCCAGCAGCAACAGCAGTCAATTCGAACTACAGCAACATCAGCAACATCAGCAACAGTTTGAACTCCAGCAGCAACAGCAGCCTCAACAACAACAACAGCAGCCTCAACATACAAACAGCAACAGCAGCCTCAACATACAAACAACAGCAACAGCACGCCTACAACAGCAGCAGCTACAACAACAGCCTTACAACGACA
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4072
PRIMER_LEFT_PENALTY=0.219884
PRIMER_RIGHT_PENALTY=0.187326
PRIMER_LEFT_SEQUENCE=CATCAGCAACAGTTTGAACTCC
PRIMER_RIGHT_SEQUENCE=TTGTTTGTATGTTGAGGCTGCT
PRIMER_LEFT=52,22
PRIMER_RIGHT=151,22
PRIMER_LEFT_TM=59.780
PRIMER_RIGHT_TM=59.813
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=0.4771
PRIMER_LEFT_1_PENALTY=0.219884
PRIMER_RIGHT_1_PENALTY=0.257221
PRIMER_LEFT_1_SEQUENCE=ATCAGCAACAGTTTGAACTCCA
PRIMER_RIGHT_1_SEQUENCE=TGTTGTAGCTGCTGCTGTTGTA
PRIMER_LEFT_1=53,22
PRIMER_RIGHT_1=188,22
PRIMER_LEFT_1_TM=59.780
PRIMER_RIGHT_1_TM=59.743
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.5377
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.317786
PRIMER_LEFT_2_SEQUENCE=CATCAGCAACAGTTTGAACTCC
PRIMER_RIGHT_2_SEQUENCE=GCTGTTGTTTGTATGTTGAGGC
PRIMER_LEFT_2=52,22
PRIMER_RIGHT_2=155,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=59.682
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=0.5377
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=0.317786
PRIMER_LEFT_3_SEQUENCE=ATCAGCAACAGTTTGAACTCCA
PRIMER_RIGHT_3_SEQUENCE=GCTGTTGTTTGTATGTTGAGGC
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=155,22
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=59.682
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=0.5377
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=0.317786
PRIMER_LEFT_4_SEQUENCE=CATCAGCAACAGTTTGAACTCC
PRIMER_RIGHT_4_SEQUENCE=GTTGTTTGTATGTTGAGGCTGC
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=152,22
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=59.682
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_00110 Ps_contig_mira-and-tgicl-ass_franssen_1291
SEQUENCE=CMAAAAACACAGCAATATTTCATTCCTCAATTTCACATCCCTGCACAAATTCAATAACTACACCAAAGGCATAATTCGATATATTTGAATACATAAACCCTAATCAATCACACCAATGTTAATTTATTGAATGATTCAAACGATAAGTATAACATCATTTTACATTACCTGAAGCATTAAATTAACACAAAACCCCTTTCTAGAAAGTAAAAGTTCTAATTATTATCGAAACAAAGGGGATTCAACAACATATTCATACAAACACACTCCAGAATAAGATGATTCATGATAGAGATTATAGAGTTGAATTAATTAATTAACTAGGGTTGCAACAACAGGGTACTTAGCGACGAACTTGGTTTCCCAATCATTGAGAACTCCGATCTCTTTCTCGGTGAGCCCATCCAGTGACGAGGTGATGTCTTCTTCGTTTTTGCTCATCTTCGCCAGTGCTCTGCTCGCGTCTCTGCCGGCGAACATAGCGTAGGCTCCGCCGGGACCGTAGAACGATTTGCCGGTGGTGACGTCGAACACGCGACCCTTCACTGCAACGTATATGGGCTTCGATGGATCTGCGCCGTTGTATGCGCTGAGTTGCTGCGTTGTAATCTCCATTTTTCTTCTTGAATCTCACACCGCACAAAGAAGCAAGGTTTCGCTATGGTTTCACCTTTCTATCCCCCGAGTACT
TARGET=307,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00111 Ps_contig_mira-and-tgicl-ass_franssen_1292
SEQUENCE=AAGCAGGTGTTCAACGCAGAGACGTCGAGCAGAGCACTGGCGAAGATGAGCAAAAACGAAGAAGACATCACCTCGTCACTGGATGGGCTCACCGAGAAAGAGATCGGAGTTCTCAATGATTGGGAAACCAAGTTCGTCGCTAAGTACCCTGTTGTTGCAACCCTAGTTAATTAATTAATTCAACTCTATAATCTCTATCATGAATCATCTTATTCTGGAGTGTGTTTGTATGAATATGTTGTTGTAATCCCCTTTGTTTCGATAATAATTAGAACTTTTACTTCTGAAAGGGTTTAGGTTGGTTAATTTAAATTAGTCTTCTAGGTAAGTAGTAAAATTAAGATGTTATTACTTAATTCGTTTGTAAGTACTATTACTAAACTAAAATTAAAACTAAACTTGGTT
TARGET=167,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3915
PRIMER_LEFT_PENALTY=0.174657
PRIMER_RIGHT_PENALTY=0.216800
PRIMER_LEFT_SEQUENCE=GAAACCAAGTTCGTCGCTAAGT
PRIMER_RIGHT_SEQUENCE=TCGAAACAAAGGGGATTACAAC
PRIMER_LEFT=123,22
PRIMER_RIGHT=261,22
PRIMER_LEFT_TM=59.825
PRIMER_RIGHT_TM=60.217
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=139
PRIMER_PAIR_PENALTY_1=0.4378
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=0.216800
PRIMER_LEFT_1_SEQUENCE=CTCAATGATTGGGAAACCAAGT
PRIMER_RIGHT_1_SEQUENCE=TCGAAACAAAGGGGATTACAAC
PRIMER_LEFT_1=111,22
PRIMER_RIGHT_1=261,22
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=60.217
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=0.5000
PRIMER_LEFT_2_PENALTY=0.283237
PRIMER_RIGHT_2_PENALTY=0.216800
PRIMER_LEFT_2_SEQUENCE=ACCAAGTTCGTCGCTAAGTACC
PRIMER_RIGHT_2_SEQUENCE=TCGAAACAAAGGGGATTACAAC
PRIMER_LEFT_2=126,22
PRIMER_RIGHT_2=261,22
PRIMER_LEFT_2_TM=59.717
PRIMER_RIGHT_2_TM=60.217
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.7899
PRIMER_LEFT_3_PENALTY=0.573098
PRIMER_RIGHT_3_PENALTY=0.216800
PRIMER_LEFT_3_SEQUENCE=CAATGATTGGGAAACCAAGTTC
PRIMER_RIGHT_3_SEQUENCE=TCGAAACAAAGGGGATTACAAC
PRIMER_LEFT_3=113,22
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=60.573
PRIMER_RIGHT_3_TM=60.217
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.8415
PRIMER_LEFT_4_PENALTY=0.624727
PRIMER_RIGHT_4_PENALTY=0.216800
PRIMER_LEFT_4_SEQUENCE=AGAAGACATCACCTCGTCACTG
PRIMER_RIGHT_4_SEQUENCE=TCGAAACAAAGGGGATTACAAC
PRIMER_LEFT_4=59,22
PRIMER_RIGHT_4=261,22
PRIMER_LEFT_4_TM=59.375
PRIMER_RIGHT_4_TM=60.217
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_00112 Ps_contig_mira-and-tgicl-ass_franssen_1312
SEQUENCE=TGGTACATATCAGATGAAGCTCAAAAGGAGCGAGTCAGTCAGATGATTGAAGATTTTGAACCTTTTGAGGGAGAGGCTCTTTTGGATGCCAAGTGCTTCAAGTGATAATTTATTATATGCAAGGTTATTATGCTCGTCTTTGTTTTTGGTATAGTTTAGTTTCTTTGTTATCTCAGTTTCAGGGAAGTTCAAGCCGATACTTCGATTTGACTTGATGTTTTCACCATAGTTTTCATTTTTGCAAGGGAAGACTAGTATCTTTTTTTAGTATGTATGAAAAATTGTGGATTCAGTGTGTTTTTTGGTCCCCTGTTTTGGTACATGGGTTCGAAGTGTTTTTTATTTGTTTTAAAAAATAAAGAAATTTGATGGAACACTTACAAAGGAGGGGAGCATGCTTGAATTCGAAATGTTGATTTATTTATTTATATTTTTATCAY
TARGET=416,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00113 Ps_contig_mira-and-tgicl-ass_franssen_1328
SEQUENCE=ATGGTACCAGAATACTAGAGANTNTATNCGGATCGTTCTGGATNTTCCCGTGTTTTATAACTACTGATCTTCTGGCTCGTGGTATTGATGTGCAGCAAGTGTCTTTAGTTATAAATTATGATCTCCCCACACAACCCGAAAACTATCTCCATCGTATTGGTCGTAGTGGAAGGTTTGGTAGGAAAGGTGTTGCAATTAACTTTGTCACGAAGGATGATGAAAGAATGCTGGGTGACATCCAGAAGTTCTACAATGTGTTGATTGAGGAGCTTCCTTCCAATGTGGCTGAACTTCTGTGATGAAATTTTTCTTCCATCATACTAGARGGAAGTTGGTTTCTGAGTAAAAAATGGTTCAATTTTGGCCACTTTATTTTCAGTTGTGAACTTTCTAATTGTGGTATTGTCAAGTAGCCGGGGCATGGCGTTGCAATTCAGAAATTTGAATACAACTTGTAATCATYCTGCAATTCTGCACTATTCAATCAATCAATTAGACGTTGTGGCATTATAAAACTTGAAGCAGAATTTAAAAAAAACCACTTATGTAATGACATTATATTACTGATCGTTTAATCTTCTACTAGTGGTGAATTT
TARGET=481,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00114 Ps_contig_mira-and-tgicl-ass_franssen_1331
SEQUENCE=AAACCAAAGGTTTACGTAAAACAAGAAAATAACAAACAAACAAGGGCGAAAAGGTAAGGGAAACGAATTAACATATAACTTAACCTTCCTAGAAACCAAACTTACCTTCTAGTATGATGGAGAAAAATTTACATCACAGAAGTTCAGCCACATTGGAAGGAAGCTCCTCAATCAACACATTGTAGAACTTCTGGATGTCACCCAGCATTCTTTCATCATCCTTCGTGACAAAGTTAATTGCAACACCTTTCCTACCAAACCTTCCACTACGACCACGATGGAGATAGTTTTCGGGTTGTGTGGGGAGGATCATAATTTATAACTAAAGACACTTGCTGCACATCAATACCACGAGCCAGAAGATCAGTAGTTATTAAAACASGGAAGATCCASAACGGAATTCCCGCATAATAATATCTCTAGTATTCTGGTCCATGTCTCCGTGTGTTGCTGAGACTGTATGGTCACGGCTTCGCATCTTGTCAGTTAGCCAATCAAACTTTACCGTACTAGGTAGTTACAACAAAAAATAGAAC
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00115 Ps_contig_mira-and-tgicl-ass_franssen_1342
SEQUENCE=CCCCGGTTTTTCGGTTCCCGGTTCGGGTTCCGTTCCCTACGGGGAAAACCGTTTTACCCCTAACTTGGTTTGGTTCGTTCCCAAACCTACCCGTTTACCTAACGTGCACCCTCCAAAACTAAGGACCCAAAACTAGGAACAATCATGCGCCTGTTGCACAGTCTCAAACACCAGGTGTTTCTTGTCTGGTGATAGAACAACATGTTGCCACTCTGTCATGGCTATGTCAGGAGGGCACATAGGGCTGTTACAAAGGGATCTGAAGGTTATCTCTCGGACTTGACCATCATATTTTTCTGCTGTATGCCACTGTCCCATCACAAGATTAGTATCCTTTCGTACTGAGCGATACATGCTAGTAAACTTTGAATCATCCTTTAACAATAAATTAGAAAAACTGCTCAGCTGTGCAGGGGAAAACATCATTGTATATACCAACTAAAGTCTTTCTTTCTTTGATTAGTTAAAAGTTTTAAGTTATACTAACGTTTCGG
TARGET=445,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00116 Ps_contig_mira-and-tgicl-ass_franssen_1353
SEQUENCE=GGKTTTGTAAGGAGTGGGTTACTGCGGTGGTGAAGCTTGAGGTGAACGAAGAAGCGGGTGGTGCTGATGTTCATTTTGAAGCTTTTCAGATGAGTGATGTGTGTATTAGGTTATTTAAGGAGGTTGGTTTGACACTGGAAGGGTTAAGGAGGATCATGATCCTTAAGTTGTCTAATAATGAAGAGNTGTGTGTGTGGTGTAAGGATACTAAGNGTTNATAATGTTATTTTTTCGTGGGTGGTTGTAA
TARGET=187,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00117 Ps_contig_mira-and-tgicl-ass_franssen_1379
SEQUENCE=ATCTTCTTCTTCTTCTTTATAACCCAATCAACAGAGAAAAAGAAGCAGTTTCAGTTTGAGATTCAGATCGGAGAAAGTGATTGGTGATTCATAGATTAGAATTATGCATCTCTAGTGGTTAGAATGGCCATAGAGTTTGTATGTCAGTTGCAAGTAACCGTTGTTATTGTTACAAAGGAAAAGAAACACACGTACCCTTTTTGTTCCGACACCGTNCAATCTCTCTTCTTTNACGTT
TARGET=2,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00118 Ps_contig_mira-and-tgicl-ass_franssen_1380
SEQUENCE=AGTACGCGGGGACTCYTCTTCTTCTTCTTCTTCTTCTTATAACCCAATCAACAGAAGAAAAAAGAAAGCAGTTTCTAGTTTGAGATTCAGATCGGAAGAAAGTGATGGTGATTCATAGAATTGAACTATGCATATCTATGGTTAGAATGGCCATAGTAGTTTGTTATGGCAGTTGCAAGGTAAACGGGTTTGTTTATTGTTACAAAGAAAAGAAACCACCCGTTACCCTTTTTGTTTCCGTTCAGCCGTGACAAAATTCCTTCCCTTCCTTTCCTTTTCACGGTTATTCTTTCGTTG
TARGET=17,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00119 Ps_contig_mira-and-tgicl-ass_franssen_1381
SEQUENCE=TTTTAAAAATTRACCTGAATCAATTCATTCATTCATTCATAATCAACATAACTTGTGCATCATAATCTTAGGAAATTAACCAAGTAGTTTAGAAATAAAAACCAAACCCTAAAAGCATGGTTGTNTCTAATTTTCCTTANATTGTTGGCATACAAATAACAACACGATGACCAACCAAACCGAAAAAGGGTAAATTNTGTACTGATACTAAATATATATACAGTAGTTTTANAGAAAC
TARGET=23,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00120 Ps_contig_mira-and-tgicl-ass_franssen_1383
SEQUENCE=GGWTAGAAATACCTGAATCAATTCATTCATTCATTCATAATCAACATAACTTGTGCATCATAATCTTGGAAATTAACCAAGAGTTTGAAATAAAAAACCAAAACCCTAAAAGCATGGTTGTATCTAATTTTCCTTTACATAGTTTGGCATCAAATACAACACGATGCACAGACAAAAAGGGAAATTNTGTACTGATCTAAACTATATATACAGTAGTTTTANAGAAACAAGAAATTAACAACAACAAACTAAG
TARGET=21,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00121 Ps_contig_mira-and-tgicl-ass_franssen_1384
SEQUENCE=CTCTTCTTCTTCTTCTTCTTCTTATAACCCAATCAACAGAGAAAAAGAAAGCGGTTTCAGTTTGAGATTCAGATCGGAGAAAGTGATGGTGATTCATAGAATTGAACTATGCATATCTATGGTTAAGAATGGACCAATAGTAGTTTGTTATGGCTAGTTGCAAGGTAAACGGTTTNTTTATTGTTACAAAGGAAAGAACCGCCGTACCTTTTGTCCTNACGCCGTGACAAATCCTCCTCTTCCTTT
TARGET=2,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00122 Ps_contig_mira-and-tgicl-ass_franssen_1385
SEQUENCE=ATCTCTTCTTCTTCTTATAACCCAATCAACAGAGAAAAAGAAAGCAGTTTCAGTTTGAGATTCAGATCGGAGAAAGTGATGGTGATTCATAGATTAGAATTATGCATCTCTATTGGTTAAGAAGTGGCCATAGAGTTTGTTATGTCAGTTGCACGAAACCGGTTTGTTTATTGTTCAAGAAAAGAAACACAGTACCCTTTTTGTTCCGTACAACCGTGCAAATACTCCTCTTCTTTCACGTTA
TARGET=4,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00123 Ps_contig_mira-and-tgicl-ass_franssen_1386
SEQUENCE=TTTAGAATGGTCCATAGAGTTGTTTATTGTCAGTTGTCAGAAACCGTTACGGTTTATTTAGTTTCTAACGAAARAAAACAACAGACCCTTTTAGTTTCCGTTRCTAACCGTAGCAAATAMTCCTCTTCCTTTTCACGGTTACCTTCGTTGATGATTTCTATCTAGCATGTTTTTTCTTCAGTTTGTGTTGTTAATTTCTGTTTCATAAAACTCTGTATATATAGTTTAGATCAGTACATAATTTTCCCTTTTGCTGTGCATCGTGTTGTATTTGATGCCAAACTATGTAAAGGAAAATTGATACAACCATGCTTTTAGGGTTTGTTTTTATTCAAACTCTTGGTTAATTTCCAAGATTATGATGCACAAGTTATGTTGATTATGAATGAATGAATGAATTGATTCAGGTATTTCTATTTCTTCAAYTTCTTCTTGTACTGTTTGCTGGTTCACTTTGCTTAATTTTCTTCTTCTTGTTTGTTAATTCTTTCATTGAAATTGGGAAAAATAAATTCTGTATTTTCTTTAG
TARGET=382,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00124 Ps_contig_mira-and-tgicl-ass_franssen_1387
SEQUENCE=CCGTGAAAGGAAGAGGAGATTTGCACGGTTGTACGGAACAAAAGGGTCTGTGTTTCTTTCTTGAACAATAACCACGGTTTCTGCAACTGACATAACAAACTCTATGGCCATTCTAACCATAGAGATGCATAATTCTAATCTATGAATCACCATCACTTTCTCCGATCTGAATCTCAAACTGAAACTGCTTTCTTTTTCTCTGTTGTTGGTTATAAGAAGAAGAAGAGATAGTACTCTGTCGTTGAACTACCCACTGTCCTTTCGTAGGAACGACCACCGACAAACGGGAGGGGTA
TARGET=214,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1137
PRIMER_LEFT_PENALTY=0.066394
PRIMER_RIGHT_PENALTY=0.047267
PRIMER_LEFT_SEQUENCE=AATAACCACGGTTTCTGCAACT
PRIMER_RIGHT_SEQUENCE=CCTACGAAAGGACAGTGGGTAG
PRIMER_LEFT=66,22
PRIMER_RIGHT=267,22
PRIMER_LEFT_TM=59.934
PRIMER_RIGHT_TM=60.047
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.1617
PRIMER_LEFT_1_PENALTY=0.114470
PRIMER_RIGHT_1_PENALTY=0.047267
PRIMER_LEFT_1_SEQUENCE=GGAACAAAAGGGTCTGTGTTTC
PRIMER_RIGHT_1_SEQUENCE=CCTACGAAAGGACAGTGGGTAG
PRIMER_LEFT_1=34,22
PRIMER_RIGHT_1=267,22
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=60.047
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.2145
PRIMER_LEFT_2_PENALTY=0.167242
PRIMER_RIGHT_2_PENALTY=0.047267
PRIMER_LEFT_2_SEQUENCE=AATCACCATCACTTTCTCCGAT
PRIMER_RIGHT_2_SEQUENCE=CCTACGAAAGGACAGTGGGTAG
PRIMER_LEFT_2=144,22
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=59.833
PRIMER_RIGHT_2_TM=60.047
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.8000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=0.2813
PRIMER_LEFT_3_PENALTY=0.234069
PRIMER_RIGHT_3_PENALTY=0.047267
PRIMER_LEFT_3_SEQUENCE=CGTGAAAGGAAGAGGAGATTTG
PRIMER_RIGHT_3_SEQUENCE=CCTACGAAAGGACAGTGGGTAG
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=267,22
PRIMER_LEFT_3_TM=60.234
PRIMER_RIGHT_3_TM=60.047
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.3706
PRIMER_LEFT_4_PENALTY=0.323355
PRIMER_RIGHT_4_PENALTY=0.047267
PRIMER_LEFT_4_SEQUENCE=CGGTTTCTGCAACTGACATAAC
PRIMER_RIGHT_4_SEQUENCE=CCTACGAAAGGACAGTGGGTAG
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=267,22
PRIMER_LEFT_4_TM=59.677
PRIMER_RIGHT_4_TM=60.047
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_00125 Ps_contig_mira-and-tgicl-ass_franssen_1388
SEQUENCE=AAAAACATGCTAGATAGAAATCATCTTAACGTAAGGTTAACCGTTGAAAAGGAAGAGGAGTATTTGCACGGTTGTACGGAACGAAAGGGTCTGTGTTTCTTTCTTGAACAATAACAACGGTTTCTGCAACTGACATAACAAACTCTATGGCCATTCTAACCATAGAGATGCRTAATTCTAATCTATGAATCACCATCACTTTCTCCGATCTGAATCTCARACTGAAACTGTCTTTTCTTTTCTCTGTTGATWGKTTAGRRGWKAAKARAGGAAAGGAAAGGAAAGAGTATTAGTTACTCGTGGCGTTTAGACTACC
TARGET=269,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00126 Ps_contig_mira-and-tgicl-ass_franssen_1389
SEQUENCE=ATCTCTTCTTCTTCTTATAACCCAATCAACAGAGAAAAAGAAAGCAGTTTCAGTTYGAGATTCAGATCGGAGAAAGTGATGGTGATTCATAGATTAGAATTATGCATCTNTATGGTTANATGGCATAGTAGTTTTGTTATGTCAGTTGACAAGTAACCGTTTGTTATTGTACAAAGAAAGAACNCACGTTACCCCTTTTTGTTACCCGAACAACCGTGAACCTAAAAATTACCCTTTCCCCCTTTCCTTTTTACCCCGTTTTTTACCGAACCGGGGTTTTTAA
TARGET=4,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00127 Ps_contig_mira-and-tgicl-ass_franssen_1395
SEQUENCE=CAGTTAAAAGATGCTGAAGAAGAGTTGGAGAAAGTCAAAAGAGGGGAAGCAAAAAGGCTTGATTTAGATTCATTGCTGTGAATCATATGAATAATGCCGTTATTGTAATTTCCTTTTGTCACTATTTTTGTTGTTGTTGAAGTTTTACATTGTTATACGAACTTCTTTANGTGTTGGATTCAAATGTAAGTCATTTATCTAGTTTTGTTCTTATTTTAATCAATAAAAAGT
TARGET=128,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00128 Ps_contig_mira-and-tgicl-ass_franssen_1406
SEQUENCE=TTCGGGGTAGAGCATTTGAAGTGTTTACGGCTGCACTCTCTCTCTCGRCTCAATAWGGCTGAACCGTCGTTNCTCGTCCCCGTCGCCGTCGAAGTCGATCCCAATCAATCGAAGTCAAGCTCTTCAACNGAGT
TARGET=36,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00129 Ps_contig_mira-and-tgicl-ass_franssen_1448
SEQUENCE=TGGAAGAGGAAGTTGAAGAAGATGAGTACGATAAGAGGGTCAAAGAGGTTCATGCAAGTGGAGAATTCCATATCAGTTAAGCACCCAAATCCTTGCATTATTTGACTAAAATTAATTTTTTAGAAGACCTACCCTTTTATTTTATCTGTTGTAGTAAACTAGAGTCACCTATYTGTGTATATTTATTTATTATTATGAACTCTAGTGTTATTTTGTTATGTGTWMTYTATGAGTCGGGACCGGACCGTA
TARGET=180,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00130 Ps_contig_mira-and-tgicl-ass_franssen_1449
SEQUENCE=TGGTAAGTAGGAAGTTGAAGAAGATGAGTACGATAAGAGGGTCAAAGAGGTTCATGCAAGTGGAGAATTCCATATCAGTTAAGCACCCAAATCCTTGCATATTGACTAAATTATTTTTAGAAGACCTACCTTTTATTTATCTGTTGTAGTAAACTAGAAGTCTACCTTATTTGTRTATTATTATTATTATTA
TARGET=176,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00131 Ps_contig_mira-and-tgicl-ass_franssen_1450
SEQUENCE=TGGTAAGTAGGWAAGTTTGTAAGWAAGATGAGTACGATAAGAGGGTCAAAGAGGTTCATGCAAGTGGAGAATTCCATATCAGTTAAGCACCCAAATCCTTGCATATTTGCTAAATATTTTTAGAGACACCCTTTATTTTATCTGTTGTGTAAACTAGAAGTCACCTATTTGTGTATATTATTATTATTATGACTCTAGTGTATTTTGTATGTGTATCATGATGGACGCCGACTATAGGAACRACGTTAGTCGAAC
TARGET=176,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00132 Ps_contig_mira-and-tgicl-ass_franssen_1451
SEQUENCE=GTGGTATCAACGCAGAGTTGGAAGAGGAAGTGAAGAAGATGAGTACGATAAGAGGGTCGAAAGAAGGTTCATGCAAGTGGAGAATTCCATATCAGTTAAGCACCCAAATCCTTGCATATTTGCTAAAATAATTTTTTAGAAGACACCYTTTATTTTATCTGTTGTGTAAACTAGAAGTCACCTATTTGTGTATATTATTATTATGACTCTAGTGATTTGTATGTGTATCATGATGGACGCCGATATGAGAACAATTG
TARGET=193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00133 Ps_contig_mira-and-tgicl-ass_franssen_1452
SEQUENCE=CCTGGCATGTAATCAGAAGATTACKGTTCTCAAACATGGCAATTTAGAATGTAATGAGACTAACATTGTTTCTCATAGTCGGCGTCCATCATGATACACATACAAAATCACTAGAGTCATAATAATAATAATATACACAATAGGTGACTTCTAGTTTACACAACAGATAAATAAGGGTGTCTTACTAAAGWATTWATTTTARRCAAATATGSAMGGAGTTTGGGTGCTTAACTGATATGGAATTCTCC
TARGET=119,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00134 Ps_contig_mira-and-tgicl-ass_franssen_1459
SEQUENCE=TACCGTAAGTCAACTGATCGAACTTGTTTTTTCCTTTTTTTAATTTTTAAAACTTAAAAAATTGAAATTATTATTATTAGGTTCTTTCTTGGTTAGCTTTAAAAATTGTAATGTTTTGTTATGCATGTATTGTATTGCAGTTGTTTCTGGATGACAGCGTAAGAAACA
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4138
PRIMER_LEFT_PENALTY=1.296517
PRIMER_RIGHT_PENALTY=0.117281
PRIMER_LEFT_SEQUENCE=CCGTAAGTCAACTGATCGAACTT
PRIMER_RIGHT_SEQUENCE=TTCTTACGCTGTCATCCAGAAA
PRIMER_LEFT=2,23
PRIMER_RIGHT=164,22
PRIMER_LEFT_TM=59.703
PRIMER_RIGHT_TM=59.883
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=1.4138
PRIMER_LEFT_1_PENALTY=1.296517
PRIMER_RIGHT_1_PENALTY=0.117281
PRIMER_LEFT_1_SEQUENCE=CCGTAAGTCAACTGATCGAACTT
PRIMER_RIGHT_1_SEQUENCE=TTTCTTACGCTGTCATCCAGAA
PRIMER_LEFT_1=2,23
PRIMER_RIGHT_1=165,22
PRIMER_LEFT_1_TM=59.703
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=1.4722
PRIMER_LEFT_2_PENALTY=1.296517
PRIMER_RIGHT_2_PENALTY=0.175676
PRIMER_LEFT_2_SEQUENCE=CCGTAAGTCAACTGATCGAACTT
PRIMER_RIGHT_2_SEQUENCE=TACGCTGTCATCCAGAAACAAC
PRIMER_LEFT_2=2,23
PRIMER_RIGHT_2=160,22
PRIMER_LEFT_2_TM=59.703
PRIMER_RIGHT_2_TM=60.176
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=1.6014
PRIMER_LEFT_3_PENALTY=1.296517
PRIMER_RIGHT_3_PENALTY=0.304841
PRIMER_LEFT_3_SEQUENCE=CCGTAAGTCAACTGATCGAACTT
PRIMER_RIGHT_3_SEQUENCE=CTTACGCTGTCATCCAGAAACA
PRIMER_LEFT_3=2,23
PRIMER_RIGHT_3=162,22
PRIMER_LEFT_3_TM=59.703
PRIMER_RIGHT_3_TM=60.305
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=1.6014
PRIMER_LEFT_4_PENALTY=1.296517
PRIMER_RIGHT_4_PENALTY=0.304841
PRIMER_LEFT_4_SEQUENCE=CCGTAAGTCAACTGATCGAACTT
PRIMER_RIGHT_4_SEQUENCE=TGTTTCTTACGCTGTCATCCAG
PRIMER_LEFT_4=2,23
PRIMER_RIGHT_4=167,22
PRIMER_LEFT_4_TM=59.703
PRIMER_RIGHT_4_TM=60.305
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_00135 Ps_contig_mira-and-tgicl-ass_franssen_1460
SEQUENCE=TATGCATGTATGTATTGTATTGTCTAGTTGTTTCTGGATGACAGCGTAAGAAACATTGCTGCGGGTAAAGAAATGGGTCTTCACACTGTTTTGGTAATTTTACAAACCWAAAGTCTTATGATGTTGATTTATTTATTTGTGATTATGAAATTAATTAATGATGTTTGTTTAGGTTGGGGAGGGGGTGGTAAAAGACAAAGTTGGGGATTATTGTAGTGGATGTGTGAATAGAGTTGCACTTGCGGAAGTG
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00136 Ps_contig_mira-and-tgicl-ass_franssen_1485
SEQUENCE=TCCAGTGGTCGTCCCAAGCATGGTCTCTGTTGTATATTTTGGCACCTCTTCCAATTGGGTTTGTTGTGTTTTTGGTTCACTTGGCTACTATTCCTATAACTGGAACTGGTATTAACCCTGCTAGGAGTCTTGGTGCTGCCATAATATAMACAAAACAAAGAAAGAACCAATAGCTTGGGACGACCACTGGATATTCTGGGTTGGACCTTTTATTGGAGCTGCCCTTGCTGCACTATATCACCAGATCGTGATTCGAGCAATTCCTTTCAAGACAAGGGGCTAATGAATCCATCATCATCATTGACATGTCTTSKTGTTTWGAATTATCTATTCGGTGTATTCATTTCTTTGTTTTATTTTTTTATCTTATGTTAGTAAATTATGAGCTCGTGTTGTATGGACTGGTGGAGGTGGAGTATTTCTTTCTTAAAACGG
TARGET=290,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00137 Ps_contig_mira-and-tgicl-ass_franssen_1494
SEQUENCE=CCTAGTTTTAGATCTCTTTATTGATTATTCATGTGATTGGTTGTTGCTGAAATTTCAAGTTATTTAAGTTTTACTTTACTTTACTTTTATCTAGATTGTTACTCATTATTTTAATTTAAAATGAAAATTAGTAAAAATTTAAAATTTAAAAAAAAMCCTTTT
TARGET=70,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00138 Ps_contig_mira-and-tgicl-ass_franssen_1535
SEQUENCE=ACCCCGTTTTAACCCTTAACCTAAACCTTTTACCTTAAACCTAAACGAAGTTTAACTAACTAACCAAAAATTTAAAACTAAAAGTTTAACGGTACCGAAAAACCTTTAACGTTAAAGGCTTTAACTCTAANCAACAACACAAAGAACCAAGTAAACTAGTACATCACTTGTACAACTGAAAATAACAATAAAATAACAAGTCAATTGTCTGGCCTCAATCTCTTGCTAAATCGATGCAGGGACAAGAGGCCGACCTCGTCCGATTGCAAAACCCCGTGTTCCATCAGGCATTCTTGGCCCAGGAAGGCGGTTTTGATGCTTCAGCGGCWATRAGGAATAAATGATGTACCTCCATGGCCCATTCCATTTGTAGCACGATATTTGTGCTTTCNCGATCTGCCTGTTTAGGGTACCTCTGCCCCACCTTTTATCCTTTGACAAATGCCTCCCCTATTCCCTTTTTCCTCAAAGAAATTATTCCTTCATGATGCTCATGTGAGTCGTGA
TARGET=53,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00139 Ps_contig_mira-and-tgicl-ass_franssen_1623
SEQUENCE=AACTGTTCCCGGAGGACCAACGGTTGCATGTAGTCACAGCAAAAGCTGTTTGAGTGGGATGCTGCTTAAAGGTCAAACAACCTCGATCCTTCTGGTAGAGCAGCACTCGGAGTTTCATGATTCAGCTTATGAAAACCATAACAAAGTCAACAAAACTGTAGAGTTTGAGAAGATTATACCACTGGAAGCCGCTCCTGTCGAGCTTGCCATCCAGGGCTAGTTTCNGCTATGGCAAGGATGACTGTGCTAGAAGAATGAAGATAATATGAAACTTANATAATGAAGTAATTTGCCCTTTCGTTTAGGTTTTTTAAAATTTTTACGGCCCGGGGGGACAAAAACTAATTGGACCTTAATTAATTAAATTAAG
TARGET=353,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00140 Ps_contig_mira-and-tgicl-ass_franssen_1625
SEQUENCE=AGTTTCCAGAACTCTCAATACATAATAGCAGGGAATGCGAGAATATTAAATCAATATMTACACAACCACAATTATAGGCTCTATTTTGATTGACTAAAGTTATAACAAAATATTATACAAATAACTGTTGACCAAATTAATACTGAGGCTGCTTCCTTTTCCCTTGTATTTATTTTTTTGAAAGAAAGAAAAAAAGGACTAGACAAAGTAAAGGATGCCATTCTGCAAGGCATGAGTAGATTCTCTATGCTGCTTCGACAAAACCACTCTGCGTTGACTACCCACTGTCCTTC
TARGET=180,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00141 Ps_contig_mira-and-tgicl-ass_franssen_1627
SEQUENCE=TGGATTTGCCGAAGCAGCATAGAGAATCTGCTCATGCCTTGCAGAATGGCATCCTTTACTTAGTTCCTTTTTTTCCTCTTACAAAAAAATNTCAAAGGGAAAGAGCAGCCTCAGTTATTATTTGGCAACAGTTTATTTGAATTAATTATTTTGTAATTAACTTTGCAATACAAAATGAGCCTATATGTGTTTGTATATATATATWKTRATTTTAATATTCTCGCTACTTTCCCTGTA
TARGET=194,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00142 Ps_contig_mira-and-tgicl-ass_franssen_1634
SEQUENCE=AAAAAAACGTTTTTTTAAACCCTTTTTTTACCGGGGGGGTTTTTCGGGGGGTTCCGGAACCGGTTACCCGTAAACCTTTACCTAAAACGGGTACCCGAACTTTAAGGGTTTAGGGTAACGGCCGTGTTTTTGGGTTAGTTGGTTAAGTTAGATTTTGTATATTGTAAGACTGGTGGTCAACATTTTGTATGAACTTGCAAGAGCACTAAATGGTTTTAGTTGCACAAAAGAAGAGTTTACTTGTCTGCAGCACCACAGTGTCCTTTCCCTGATGCTCATTTGGATTCAGCTATTAACACTGGTTTGTTTGATTACGTTTTGGGTTTCAGTTTTACAATAATCCTCAGTGTCAATACTCGAATGGAAACACTAACAATCTTGTTAATGCTTGGACTCAATGGACTTCAAGTCAAGACTAAAACAAGTGTTCTTAGGTGTTCCTGCTAATGCTGCTGCTGCCTACCAAGGTGGTGTCTTTATTCCTTNTGTATTTGGTTTTTGTACTTTTCCTACTAACGTTTCCTTCCTGACTTTAAGTTTAAAGGTACGTTTTCCCTTAACCCTTAAAAAAGTTTACGGTTT
TARGET=448,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3386
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.335045
PRIMER_LEFT_SEQUENCE=TGTTAATGCTTGGACTCAATGG
PRIMER_RIGHT_SEQUENCE=AAGGGTTAAGGGAAAACGTACC
PRIMER_LEFT=379,22
PRIMER_RIGHT=564,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.665
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=186
PRIMER_PAIR_PENALTY_1=0.3394
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.335045
PRIMER_LEFT_1_SEQUENCE=AATGCTTGGACTCAATGGACTT
PRIMER_RIGHT_1_SEQUENCE=AAGGGTTAAGGGAAAACGTACC
PRIMER_LEFT_1=383,22
PRIMER_RIGHT_1=564,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.665
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=0.6148
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.611210
PRIMER_LEFT_2_SEQUENCE=TGTTAATGCTTGGACTCAATGG
PRIMER_RIGHT_2_SEQUENCE=AGGAATAAAGACACCACCTTGG
PRIMER_LEFT_2=379,22
PRIMER_RIGHT_2=483,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.389
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=0.6156
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.611210
PRIMER_LEFT_3_SEQUENCE=AATGCTTGGACTCAATGGACTT
PRIMER_RIGHT_3_SEQUENCE=AGGAATAAAGACACCACCTTGG
PRIMER_LEFT_3=383,22
PRIMER_RIGHT_3=483,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=59.389
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=1.0113
PRIMER_LEFT_4_PENALTY=0.676221
PRIMER_RIGHT_4_PENALTY=0.335045
PRIMER_LEFT_4_SEQUENCE=GATGCTCATTTGGATTCAGCTA
PRIMER_RIGHT_4_SEQUENCE=AAGGGTTAAGGGAAAACGTACC
PRIMER_LEFT_4=270,22
PRIMER_RIGHT_4=564,22
PRIMER_LEFT_4_TM=59.324
PRIMER_RIGHT_4_TM=59.665
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_00143 Ps_contig_mira-and-tgicl-ass_franssen_1635
SEQUENCE=CGGAGCTAAGAGAGTAGCTTCCAGCGCCGCCTCCGAGAGAGAGAAGAACTTTGATTCCTTTCGCTTGACATGCTTGAATCTCGGAACTAAACTTTGTGCATTCATTGGCACTTGGATTACAATGGCCAGCTAAGGTTTTAGTTGGCGGGGTTTGCCATTGCCGAAGTGGAGAGAAAGCTTATTGTTTAACAAAGTTTGGTAGTTTGTTTGTGTTGCAAGCATC
TARGET=35,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0693
PRIMER_LEFT_PENALTY=0.045851
PRIMER_RIGHT_PENALTY=0.023406
PRIMER_LEFT_SEQUENCE=GCTAAGAGAGTAGCTTCCAGCG
PRIMER_RIGHT_SEQUENCE=TAAAACCTTAGCTGGCCATTGT
PRIMER_LEFT=4,22
PRIMER_RIGHT=139,22
PRIMER_LEFT_TM=59.954
PRIMER_RIGHT_TM=60.023
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=0.0693
PRIMER_LEFT_1_PENALTY=0.045851
PRIMER_RIGHT_1_PENALTY=0.023406
PRIMER_LEFT_1_SEQUENCE=GCTAAGAGAGTAGCTTCCAGCG
PRIMER_RIGHT_1_SEQUENCE=AAAACCTTAGCTGGCCATTGTA
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=138,22
PRIMER_LEFT_1_TM=59.954
PRIMER_RIGHT_1_TM=60.023
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=0.1866
PRIMER_LEFT_2_PENALTY=0.045851
PRIMER_RIGHT_2_PENALTY=0.140702
PRIMER_LEFT_2_SEQUENCE=GCTAAGAGAGTAGCTTCCAGCG
PRIMER_RIGHT_2_SEQUENCE=CTAAAACCTTAGCTGGCCATTG
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=140,22
PRIMER_LEFT_2_TM=59.954
PRIMER_RIGHT_2_TM=60.141
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=0.2894
PRIMER_LEFT_3_PENALTY=0.045851
PRIMER_RIGHT_3_PENALTY=0.243575
PRIMER_LEFT_3_SEQUENCE=GCTAAGAGAGTAGCTTCCAGCG
PRIMER_RIGHT_3_SEQUENCE=CATTGTAATCCAAGTGCCAATG
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=123,22
PRIMER_LEFT_3_TM=59.954
PRIMER_RIGHT_3_TM=60.244
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=0.5182
PRIMER_LEFT_4_PENALTY=0.045851
PRIMER_RIGHT_4_PENALTY=0.472356
PRIMER_LEFT_4_SEQUENCE=GCTAAGAGAGTAGCTTCCAGCG
PRIMER_RIGHT_4_SEQUENCE=ACCTTAGCTGGCCATTGTAATC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=135,22
PRIMER_LEFT_4_TM=59.954
PRIMER_RIGHT_4_TM=59.528
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_00144 Ps_contig_mira-and-tgicl-ass_franssen_1637
SEQUENCE=CAATAGTCAAGCAAGGAAAGTCAAAGTACTTCTCTCTCTCGAGGCGGCGCCGGAAGGCTACTCTCTTAGCTCCGCCTGACGATGCTACACAAGTTGCAAACTATCTATGGAACAATTTCTTGGGTGGCACGTCAACTTCAAGGCCATTAGGTGACGCTGTTTTGGATGGAATTGATTTTGATATTGAAGCTGGTGGTCAACATTTTGATGAACTTGCAAGAGCACTAAATGGTTTTAGTGCACAAAGAGGGTTTACTTGTAACTGCAGCACCACAGTGTCCTTTCCCTGATGCTCATTTGGATTCAGCTATTAACACTGGTTTGTTTGATTACGTTTGGGTTCAGTTTTACAATAATCCTCAGTGTCAATACTCGAATGGAAACACTAACAATCTTGTTAATGCTTGGACTCAATGGACTTCAAGTCAAGACTAAACAAGTGTTCTTAGGTGTTCCTGACTAATGCTGCTGCTGCTCAGTGGTGTCTTTATTCCTTNTGGTATTTGGTTTTGTACTTTTCCTACCGTTAACGTTTCCTTCCTGTACTTAAGTTAAGT
TARGET=31,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.2960
PRIMER_LEFT_PENALTY=2.194342
PRIMER_RIGHT_PENALTY=0.101680
PRIMER_LEFT_SEQUENCE=AGTCAAGCAAGGAAAGTCAAAG
PRIMER_RIGHT_SEQUENCE=ATCCAAAACAGCGTCACCTAAT
PRIMER_LEFT=4,22
PRIMER_RIGHT=166,22
PRIMER_LEFT_TM=57.806
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=2.4401
PRIMER_LEFT_1_PENALTY=2.338413
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=GTCAAGCAAGGAAAGTCAAAGT
PRIMER_RIGHT_1_SEQUENCE=ATCCAAAACAGCGTCACCTAAT
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=166,22
PRIMER_LEFT_1_TM=57.662
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=2.6061
PRIMER_LEFT_2_PENALTY=2.194342
PRIMER_RIGHT_2_PENALTY=0.411756
PRIMER_LEFT_2_SEQUENCE=AGTCAAGCAAGGAAAGTCAAAG
PRIMER_RIGHT_2_SEQUENCE=CTGTGGTGCTGCAGTTACAAGT
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=275,22
PRIMER_LEFT_2_TM=57.806
PRIMER_RIGHT_2_TM=60.412
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=2.6225
PRIMER_LEFT_3_PENALTY=2.194342
PRIMER_RIGHT_3_PENALTY=0.428205
PRIMER_LEFT_3_SEQUENCE=AGTCAAGCAAGGAAAGTCAAAG
PRIMER_RIGHT_3_SEQUENCE=GTGCTCTTGCAAGTTCATCAAA
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=224,22
PRIMER_LEFT_3_TM=57.806
PRIMER_RIGHT_3_TM=60.428
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=2.6682
PRIMER_LEFT_4_PENALTY=2.194342
PRIMER_RIGHT_4_PENALTY=0.473868
PRIMER_LEFT_4_SEQUENCE=AGTCAAGCAAGGAAAGTCAAAG
PRIMER_RIGHT_4_SEQUENCE=ACCTAATGGCCTTGAAGTTGAC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=151,22
PRIMER_LEFT_4_TM=57.806
PRIMER_RIGHT_4_TM=59.526
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_00145 Ps_contig_mira-and-tgicl-ass_franssen_1637
SEQUENCE=CAATAGTCAAGCAAGGAAAGTCAAAGTACTTCTCTCTCTCGAGGCGGCGCCGGAAGGCTACTCTCTTAGCTCCGCCTGACGATGCTACACAAGTTGCAAACTATCTATGGAACAATTTCTTGGGTGGCACGTCAACTTCAAGGCCATTAGGTGACGCTGTTTTGGATGGAATTGATTTTGATATTGAAGCTGGTGGTCAACATTTTGATGAACTTGCAAGAGCACTAAATGGTTTTAGTGCACAAAGAGGGTTTACTTGTAACTGCAGCACCACAGTGTCCTTTCCCTGATGCTCATTTGGATTCAGCTATTAACACTGGTTTGTTTGATTACGTTTGGGTTCAGTTTTACAATAATCCTCAGTGTCAATACTCGAATGGAAACACTAACAATCTTGTTAATGCTTGGACTCAATGGACTTCAAGTCAAGACTAAACAAGTGTTCTTAGGTGTTCCTGACTAATGCTGCTGCTGCTCAGTGGTGTCTTTATTCCTTNTGGTATTTGGTTTTGTACTTTTCCTACCGTTAACGTTTCCTTCCTGTACTTAAGTTAAGT
TARGET=464,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4641
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.460533
PRIMER_LEFT_SEQUENCE=TGTTAATGCTTGGACTCAATGG
PRIMER_RIGHT_SEQUENCE=GAAGGAAACGTTAACGGTAGGA
PRIMER_LEFT=395,22
PRIMER_RIGHT=539,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.539
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=0.4649
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.460533
PRIMER_LEFT_1_SEQUENCE=AATGCTTGGACTCAATGGACTT
PRIMER_RIGHT_1_SEQUENCE=GAAGGAAACGTTAACGGTAGGA
PRIMER_LEFT_1=399,22
PRIMER_RIGHT_1=539,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.539
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.5713
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.567736
PRIMER_LEFT_2_SEQUENCE=TGTTAATGCTTGGACTCAATGG
PRIMER_RIGHT_2_SEQUENCE=GGAAACGTTAACGGTAGGAAAA
PRIMER_LEFT_2=395,22
PRIMER_RIGHT_2=536,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.432
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.5721
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.567736
PRIMER_LEFT_3_SEQUENCE=AATGCTTGGACTCAATGGACTT
PRIMER_RIGHT_3_SEQUENCE=GGAAACGTTAACGGTAGGAAAA
PRIMER_LEFT_3=399,22
PRIMER_RIGHT_3=536,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=59.432
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=0.9059
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.902354
PRIMER_LEFT_4_SEQUENCE=TGTTAATGCTTGGACTCAATGG
PRIMER_RIGHT_4_SEQUENCE=AGGAAACGTTAACGGTAGGAAA
PRIMER_LEFT_4=395,22
PRIMER_RIGHT_4=537,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=59.098
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_00146 Ps_contig_mira-and-tgicl-ass_franssen_1639
SEQUENCE=TTTAGTGCTCTTGCAAGTTCATCAAAATTTGTTTTGACCACCAGCYTCAATATCAAAWWCAATTCCATCCAAAACAGCGTCACCTAATGGCCTTGAAGTTGACGTGCCACCCAAGAAATTGTTCCATAGATAGTTTGCAACTTGTGTAGCATCATCAGCGGAGCTAAGAGAGTAGCTTCCGGCGCCGCCTCCGAGAGAGAGAAGAACTTTGATTTCCTTTCGCTTGACATGCTTGAATCTACGGTAAACTTAAACTTTGGGCTATTTCAGTTGGACCTAACGTTTGGGTAATTTAACAAGTTCGGGCCCTAAGGTCCGTTTAAAAGGGGTTTTTTAGGGTTTTCGGGGTTACCGGGGGGGGTTTTTTACGGACCCC
TARGET=193,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00147 Ps_contig_mira-and-tgicl-ass_franssen_1667
SEQUENCE=TTGNTACGGAGANAATCTTGACTNTTACTCTCAGAGGCCATTCGGATCTTCACTCTCGTTGTCATCAANCCTTCTCGACACCATCGCAGCAGTCCTTAGTATTTGGCTTTTTYCTTCAAAAATAAAATAAATGTGAATCACGGTTCACCTACTTCGGTTGACTCATCTATATATGAGYGTGACACATGTATAATATACTACTACTACTATCAATGGATTTCAATCCTTACTCGACTCACGCATTACTTTTCAAATCAAATAACACTGTGTCTTATTCTTTTAAAGTAAAAAAAGT
TARGET=196,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00148 Ps_contig_mira-and-tgicl-ass_franssen_1671
SEQUENCE=TACTCGAATTTGGCTGCCATACAATGCAACAAAACTAAAAGAAGAGTAAAAAAGGAAGTAGTAGCTGAAATGAAAATAGTGATTTGTTGTTGTTGTTGGTCT
TARGET=84,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00149 Ps_contig_mira-and-tgicl-ass_franssen_1716
SEQUENCE=TTYTTGTATGAATAATATCAATTTATATAATAGTCAACCGAAGAAAAGATAGGAAATTTAAGAATTGTGAGACAAAAAAACAACATAACATAACATTATTAACTTCACATGTTACTACAAACAGGTGAAGGAATTAATAGAGTTACTACTCATTGTAGAAACAAACATAACATGATATCATATCATAGCATAGCAGTAACAGAAATAGTAAAAATTAAGCAGTGAGATCTTCTTCTGTGNGTCTCAATAAACAACATCAAGAGGGTAGGANANCAACANAAGTAANAAA
TARGET=82,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00150 Ps_contig_mira-and-tgicl-ass_franssen_1736
SEQUENCE=TCGGGGAAAGACACCAACACATTTCCCATCATCATCTTCTTCTTCTCCGATAGGAAACTTTCAGTTCTCCAACCCCCCCAAAAAAACTAGACCAAACCCCTTAAAACGTTCTTCGATATGACCGTCGGAGGCCAACCCTCTGGCCGTATTGTCATGGAGCTTTACGCTGATACCACTCCTCTCACCGNTGATTAACCTTCCGTGCACTCTGCCACCGGNGAAGAAAGGNGTCGCATAGGCGGGAA
TARGET=35,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6188
PRIMER_LEFT_PENALTY=0.516652
PRIMER_RIGHT_PENALTY=0.102129
PRIMER_LEFT_SEQUENCE=AAGACACCAACACATTTCCCAT
PRIMER_RIGHT_SEQUENCE=TGAGAGGAGTGGTATCAGCGTA
PRIMER_LEFT=7,22
PRIMER_RIGHT=183,22
PRIMER_LEFT_TM=60.517
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=8.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.6567
PRIMER_LEFT_1_PENALTY=0.516652
PRIMER_RIGHT_1_PENALTY=0.140098
PRIMER_LEFT_1_SEQUENCE=AAGACACCAACACATTTCCCAT
PRIMER_RIGHT_1_SEQUENCE=AGAACGTTTTAAGGGGTTTGGT
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=111,22
PRIMER_LEFT_1_TM=60.517
PRIMER_RIGHT_1_TM=60.140
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=0.6567
PRIMER_LEFT_2_PENALTY=0.516652
PRIMER_RIGHT_2_PENALTY=0.140098
PRIMER_LEFT_2_SEQUENCE=AAGACACCAACACATTTCCCAT
PRIMER_RIGHT_2_SEQUENCE=AACGTTTTAAGGGGTTTGGTCT
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=109,22
PRIMER_LEFT_2_TM=60.517
PRIMER_RIGHT_2_TM=60.140
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.7065
PRIMER_LEFT_3_PENALTY=0.516652
PRIMER_RIGHT_3_PENALTY=0.189810
PRIMER_LEFT_3_SEQUENCE=AAGACACCAACACATTTCCCAT
PRIMER_RIGHT_3_SEQUENCE=AGTGGTATCAGCGTAAAGCTCC
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=176,22
PRIMER_LEFT_3_TM=60.517
PRIMER_RIGHT_3_TM=59.810
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=0.7065
PRIMER_LEFT_4_PENALTY=0.516652
PRIMER_RIGHT_4_PENALTY=0.189810
PRIMER_LEFT_4_SEQUENCE=AAGACACCAACACATTTCCCAT
PRIMER_RIGHT_4_SEQUENCE=AGGAGTGGTATCAGCGTAAAGC
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=179,22
PRIMER_LEFT_4_TM=60.517
PRIMER_RIGHT_4_TM=59.810
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_00151 Ps_contig_mira-and-tgicl-ass_franssen_1741
SEQUENCE=ATCTTAGTCGATTGAATACAGGTTACACATATCTAAATATTTACATTGCAACACACAAATAATTGGCTGAGTTWRTAATAACAAAAAGGTCACAATTTCTTGTTAACTTCAAACATTGTTCCATGCCTAAATAGATGATTTCAATACCAACATTAAGTTAGCACCAAATGAAATTGAACCTAGATTTCTGAAACAAGATCTCAGATTTGACTCTCATGAATGAATAACATGTTAACTGTTTTATTCACAACTAAACTTGCAGCTATATATATTGTAGTTTTCACGAGATTCTAAATTACTAGAGACAATTATGCAGGAAATCAATGAAGGAATTTGCTTTGAGACTAGAGAAACTAGCAGAGGAGCTACTAGACTTGTTATGTGAAAATCTTGGACTAGAAAAAGGGTACCTCAAAAAGGCCTTCTATGGATCAAAAGGACCAACTTTTGGCACCAAGATAGCAAACTACCCTCCATGTCCAAAACCCGATCTAGTGAAGGGTCTCCGCGCACACACCGATGCCGGAGGAATCATCCTCCTTTTCCAAGACGACAAAGTCAGTGGCCTCCAACTTCTCAAAGATGGCATCTGGGTAGATGTTCCTCCCATGCATCATTCAATTGTCATCAACCTCGGTGACCAACTAGAGGTAATTACCAATGGTAAATACAAAAGTATAGAGCACCGTGTGATAGCACAAAGTGATGGAACAAGAATGTCCATAGCCTCATTCTACAACCCTGGTAGTGACGCTGTTATCTACCCAGCACCAACATTGATAGAAGAAAACAATGAAATTTACCCAAAATTTGTGTTTGAGGATTACATGAAACTCTATGCTGGGTTAAAGTTTCAGGCCAAAGAACCAAGATTTGAAGCATTTAAGGAATCAAATGTTGTTAATTTGGGACCAATTGCAACAGTTTAATTATGTGTTACTATAAATAAATATATTAAACATGTTTGTGTAAGGGGAAGCAAGCAAAGTAAAAAAAGGTTAAAGTGAAGTGTATTAGTTTCTCAAATTAGTATATATAGTTTTGTTTACTTATAGTGAGTGATGTGATGTTATTCCTTGTAACGTGGAGAAAATATAATAGTATGAAATGTTCTTAATAATCATAATAATAGTATTGTTTGATTGGAGTGGAGTGGAGAAATAAWTWTTATTGGTTAATGTCTATCTAGTCATTCTA
TARGET=1144,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00152 Ps_contig_mira-and-tgicl-ass_franssen_1772
SEQUENCE=TAATAAACCACTCAATAGCAATACAAACAAAATAAAAATTGAAAATCACCAAAAGTACAATATATTAATGTTACCATAAAATGCCAATGAAGATAATTACAATCAACAAAGTGRAGGAAGAGGCTTCCAAAGGCCATCCAAAGAGTGTTTCATACACACAATCAACTCAACACAAAAACTTAGAAGAAAAAAAAGGCCTAAACATTTAACAAAAGCATCTGCATTGATTCACTCATGAGCAACAACAACAACTTGTTTACCAACATTTTGACCAGTAAACAAGCCAATCAATGCAGCAGGACCATTCTCAAGACCCTCAGCTACGTCTTCCACATACGCAATCTTTCCTTCTCTTATAAGAGGCAAAACAAACTCCAAAAACTTTGTATAAAGATGATAGTACTCAAATACAGCAAAACCTTCCACTCGAACCCTCTTATATATGATATGTGCCAAATTCGTTACACCTTCGGGTTGATCAAGATTATACTGTGAAATCATTCCACACACAGGTATGCGACCGTGGACTCTCATATTCAAAAGTACAGCATCAAGTGTCTTTCCCCCAACATTCTCAAAGTAAATGTCAATGCCTTCTGGAAAGTATTTTTTCAAAACTGCATTGAGGTCTGGTTCTTCTTTGTAGTTAAAGGCTTCATCATATCCAAATTTATTCTTCAACAGATCCACTTTTTCTTTAGTTCCGGCACTTCCAACCACATAGCAACCGTGCAATTTAGCGAATTGTCCAACAAGTTGACCAACAGCACCAGATGCAGCCGAAACGAAAACATTCTCTCCTTTCTTAGGACTTCCTACTTCAAAGAAACCTGCATAAGCAGTCATTCCCGGCATACCGAGAATTCCAGTATAGTAGGAAAGAGGAACATCAGTGTGCTCAATTTTGAAAAGTATTTGAGCTGTAGGGATCAAGCTGTACTCTTCCCATTTAGTCATACCCCAAACTAAATCATCTTTCTTATAATCTGGATCTCTAGATTCTACAACTTTAGACACTCCAAAACCTGTCAATGGCGAACCAGGGGTAAGAGCACCAAGTTCTGCAGTAGTATCCTTGGTCATAAGAATCCGCAGGTAAGGGTCACAAGACAAGTAGAGATTTTTGAGAAGAACAAGATCGTTGGAACCTTCTGGAATCTTAAGAGTTATGGTACTGTCAACTATGTTCATGTCTGATTGTTTAGGAAAACCAGTAACATAGTCCGTGAGAATCACTTGCTTGTTCCTGCTCACTTGTGCCATGTTTGGAATTGTGTAACAAATGTATTCACTGTTGGTTCTGCTGTTGGTGGTGTAGTGTGGTGTGGTGTTTATGCTGTACTTTCTCGCTGTGTAGTGTATGTAAGAGTTATGTACACGGTACGCACATGTACCTCCGATAATACTCCCC
TARGET=240,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00153 Ps_contig_mira-and-tgicl-ass_franssen_1774
SEQUENCE=ACTTGATGCTGTACTTTTGAATATGTAGAGTCCATGGTCGCATACCTGTGTGTGGAATGATTTCACAGTATAATCTTGATCAACCTGAAGGTGTAACGAATTTGGCACATATCATATATAAGAGGGTTAGAGTGGAAGGTTTTGCTGTATTTGAGTACTATCATCTTTATACAAAGTTTTTGGAGTTTGTTTTGCCTCTTATAAGAGAAGGAAAGATTGCGTATGTGGAAGACGTAGCTGAGGGTCTTGAGAATGGTCCTGCTGCATTGATTGGCTTGTTTACTGGTCAAAATGTTGGTAAACAAGTTGTTGTTGTTGCTCATGAGTGAATCAATATAGATGCTTTTGTTAAATGTTTAGGCCTTTTTTTTCTTCAAGTTTTTGTGTTGAGTTGAGTGTGTATGAAACACTCTTTGGATGGCCTTTGGAAGCCTCTTCCTTCACTTTGTTGATTGTAATTATCTTCATTGGCATTTTATGGTAACATTAATATATTGTACTTTTGGTGATTTTCAAAMAAAA
TARGET=306,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00154 Ps_contig_mira-and-tgicl-ass_franssen_1882
SEQUENCE=GGGTCGAATAACAATGTTGTTGTCCTCATCTATGTTTGTATAGATGGTGCTTAAACGTAATAGCCTTGTGAACATGAAATTAAACTAAAAAGAAACAAGAAGATGCAAATATGTTTCGTAAGAAGCTTCATGACTAATTAACAAGTACTATACATAGAGAGAGAGAGAAGAGAGTTATGGAAAAGTGATGATGAAGAGAGTGATAYAAAGAGAAAGTTTTTGTTGGTTCTCCAGCTGGGGTGTAAACATCACAAATCACAAGAATATTATTCCCTCTTCTAGAAATGGGTCCTTGAAAATAGCTTGTGGATATAAAATAACTTCACTATCTTTTCCTGGTGCTTGATCTGTGCTAGACCCATCATAGTTCCACTTAGGTAAGTTTTGCAGGGTCACTCACTGGTCCAGGAAGAGT
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00155 Ps_contig_mira-and-tgicl-ass_franssen_1918
SEQUENCE=TACTGGACAAGTTGCATTTGAAGTGACATAAGTAGAAACAAACAAACTACCCCAATCAATACCCTCTTAACGGCACCCAAACCTCTACTACCCATGACCAAAGCATCAAGCTTCAAATCTCCAACAGACTCACAAATWTTCTCTCTTGCATCTCCCCAATAAACCTTCGCCACCACAGTCACCTGTTTCTGATCAGCCGCAGYATCGAGTACATCCAAAACGTCAGCATCAGGCACCACCTCATACTGGTGAGTCACGTTCTTCTCACTTTTTTGTTAGTTTGATGGCAACTTTGTTTTTGTTTTTTATATTATCTTATTATTATCCCCCCTAATCCCCTNTTGTTAGATTTGAGATTA
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00156 Ps_contig_mira-and-tgicl-ass_franssen_1954
SEQUENCE=TATAATTGAATTTTACTTTCTACCAACTTTTTTAATTTCCTAAGCGGACGAATAGGGTCCTTCAGTTGCCCTGGTATCTGCTCCAAGAATGATACCATCCTGAAAACTAAACCAACAATAGTGGTTCCAGTCTTCAAATAAGAAGGGGGTTTAAGACCCTTCTTTTCAAGCATATCATTTCTTCTGCAGAGATCGAAACTGAACCCACCCTTAGGAGGAAGATCCAATGATTTAGTCATACTCTAAACTCGAATTGAACCTGAAAATAAACCGATACCAAAAAGATACGCATCGTCAAAAAATAAAATCATAATACAAAACCCTTGATTTCGGTGATTTGAAAATGAAGTGTTTGAGAGAGAGAGAGAGAGAGAGAG
TARGET=355,22
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00157 Ps_contig_mira-and-tgicl-ass_franssen_1964
SEQUENCE=GTCGCTCAAAAGGATATGGTTTTGTTAGGTTCGGTGATGAAAATGAAAGGACAAGGGCAATGACTGAAATGAACGGCGTTTATTGTTCTAGCAGACCTATGCGAGTCGGTGTTGCAACGCCCAAAAAAAACTTACGGCAATCCACAACAATATTCTTCACAAGTGTTGGCTGGCGGTGGTCACTCTGCTAATGGTGCTGGTCGGCCCGAAGGCCTACCCAATCTAGGGAAGGGGTATTACTAAACAACAACAACTATTATTTGTGGAGGGTCTTGTATTCTAAG
TARGET=243,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3862
PRIMER_LEFT_PENALTY=0.110393
PRIMER_RIGHT_PENALTY=2.275811
PRIMER_LEFT_SEQUENCE=CTTACGGCAATCCACAACAATA
PRIMER_RIGHT_SEQUENCE=AGAATACAAGACCCTCCACAAA
PRIMER_LEFT=130,22
PRIMER_RIGHT=280,22
PRIMER_LEFT_TM=59.890
PRIMER_RIGHT_TM=57.724
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=2.4299
PRIMER_LEFT_1_PENALTY=0.154110
PRIMER_RIGHT_1_PENALTY=2.275811
PRIMER_LEFT_1_SEQUENCE=GAACGGCGTTTATTGTTCTAGC
PRIMER_RIGHT_1_SEQUENCE=AGAATACAAGACCCTCCACAAA
PRIMER_LEFT_1=70,22
PRIMER_RIGHT_1=280,22
PRIMER_LEFT_1_TM=60.154
PRIMER_RIGHT_1_TM=57.724
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=2.4616
PRIMER_LEFT_2_PENALTY=0.185822
PRIMER_RIGHT_2_PENALTY=2.275811
PRIMER_LEFT_2_SEQUENCE=GATGAAAATGAAAGGACAAGGG
PRIMER_RIGHT_2_SEQUENCE=AGAATACAAGACCCTCCACAAA
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=280,22
PRIMER_LEFT_2_TM=59.814
PRIMER_RIGHT_2_TM=57.724
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=2.5520
PRIMER_LEFT_3_PENALTY=0.276218
PRIMER_RIGHT_3_PENALTY=2.275811
PRIMER_LEFT_3_SEQUENCE=AAAACTTACGGCAATCCACAAC
PRIMER_RIGHT_3_SEQUENCE=AGAATACAAGACCCTCCACAAA
PRIMER_LEFT_3=126,22
PRIMER_RIGHT_3=280,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=57.724
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=2.6361
PRIMER_LEFT_4_PENALTY=0.360320
PRIMER_RIGHT_4_PENALTY=2.275811
PRIMER_LEFT_4_SEQUENCE=AAGGGCAATGACTGAAATGAAC
PRIMER_RIGHT_4_SEQUENCE=AGAATACAAGACCCTCCACAAA
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=280,22
PRIMER_LEFT_4_TM=60.360
PRIMER_RIGHT_4_TM=57.724
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_00158 Ps_contig_mira-and-tgicl-ass_franssen_1978
SEQUENCE=TATGGTAGAAAGCCTAGATTTCTTTGAGGCTATTAAATCAGGAAGATTATGAGATAAGCTGATTCAACRATAATTCCAAAAGTGTTAATGGTGATGAAGAAGAAGAGCAGCTTCTCTTTTCACAAATGCATAGTAAATCCAAAGCATTGCACTGAAAAGTGCAACAACATAAGGAAGTGACTGAAATCCTTCAGCAGATTTTTTCTTGAAGATTATGTAAAAAGTTGGCACAGGTGAAAGAAACACTGCAAAGGAAATGATGTTGCCTAGAAGGCCAAAGACAAAAGCCAAAGTTTCACGATGCATGGCCWATTTCTTGAATCTAAAGTAAAAACTGTTAGAAACTTTGGAAATTAATTTAAGAATAAGTGTCACTCTTCTTTCTTTCTACAAAGTGACTACCTAAAACCCTTTAGKTAGAAGGTATCTACTTTTNTTTCTCTCTACTAAACTTCCCNCCCCG
TARGET=378,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00159 Ps_contig_mira-and-tgicl-ass_franssen_2017
SEQUENCE=CAGGGGAAGACCGTTGGAGCTGRTGTTATTGGAGCTATCATTGAGTGAATGAAGATTTCTATCCAACGATGCAGCACCACTCTTTAAACGGGGTTCTTTATTTATTTATTTTTAATTGCAATCCATTAATTGACTTTTGTCCCTTTTTATACARCTTGTCTGTGGTAATTATAGGTTAATTTAACTTCGGGTATGATTCTTTAAGTCAAATTAGGGTTACGTTATTTTTCCTATCAGAATGTTTTGTTTGTTGTTACTTAG
TARGET=97,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00160 Ps_contig_mira-and-tgicl-ass_franssen_2021
SEQUENCE=GTCGGGATCATCAAACATAATACCTCAAATTCTAACAACCTTACCTTAAACCTTCTCAACCCAACAACAACAACAACAACATGGGCAGTGTTGAAATTCCGACAAAGGTTCTTACCAACACATCTGCTCAAATTAAGATGCCTGTGGTTGGAATGGGATCAGCACCTGACTTCACATGCAAGAAAGACACCAAAGAAGCAATCATTGAAGCCATCAAACAAGGTTACAGACACTTTGATACTGCTGCTGCTTATGGCTCTGAACAAGCTCTTGGTGAAGCTTTGAAYGAGGCTATTCAACTTGGTCTTGTCACTAGAGAACAGCTTTTTGTTACTTCCAAACTTTGGGTTACTGAAAATCAAATACATTGCAGTCGGGAATGAGATTCACGCAGATTCGCCCGAAGCAAGTTCGGTTCTTCCCGCATTGCAAAACATTCAAAACGCGATATCT
TARGET=62,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00161 Ps_contig_mira-and-tgicl-ass_franssen_2028
SEQUENCE=CCGGTTGCAGTTCAGCCGANCCCCTTTGGTCGCGGTGGTGGCGGCGCTGGTGCTCCTGGAAGGAGGTTTAAGTTTAAGTCTTTTTCTTAATTTTGTTATGTCAAGACTATTAGATTTGTTTTACTTTTGAAAAATGTTTTGTTATGGTTGAACTGGTTTAAGTTATTGTAATGGAATTTAATATATATATTTTTCATTCTGAATTGTATGGAATTGTATGAATGATGCTAGTTTTATGACTTTGAACT
TARGET=181,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00162 Ps_contig_mira-and-tgicl-ass_franssen_2050
SEQUENCE=TCGTTGGCCTTCGTCCCGTATCCCCCTCCGTTTCGTCCGGTGGTCGTTCCTAGCCTACGCACCGAACCTACCAACACCCAAACTAGGTCAGGTCGATGAACAAGGTTGAAAGACGCTTGTCGGTGGAACTGAAGTAGAAGACATGACTTTCCAACTCCAGTGTCTCCGATGATGATATACTTGAAGAGATATGCGTAAGACATTGTTGTGTGCGAGAGAGAACGAGAAGGAAGAGGAAAGAAAGAAAAGCGAAGGAGGGAGAAGGAGAAGGAAAAGAGAGAAGAANAAAATTAGAAATCGATTATCGAGATACAAATCGTAATTATACGTCCGTTCCAGGCGAGGACTAAATTAA
TARGET=236,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2605
PRIMER_LEFT_PENALTY=0.171757
PRIMER_RIGHT_PENALTY=0.088755
PRIMER_LEFT_SEQUENCE=ATGAACAAGGTTGAAAGACGCT
PRIMER_RIGHT_SEQUENCE=TTAATTTAGTCCTCGCCTGGAA
PRIMER_LEFT=95,22
PRIMER_RIGHT=354,22
PRIMER_LEFT_TM=60.172
PRIMER_RIGHT_TM=60.089
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.3148
PRIMER_LEFT_1_PENALTY=0.225996
PRIMER_RIGHT_1_PENALTY=0.088755
PRIMER_LEFT_1_SEQUENCE=TGTCGGTGGAACTGAAGTAGAA
PRIMER_RIGHT_1_SEQUENCE=TTAATTTAGTCCTCGCCTGGAA
PRIMER_LEFT_1=117,22
PRIMER_RIGHT_1=354,22
PRIMER_LEFT_1_TM=59.774
PRIMER_RIGHT_1_TM=60.089
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.3148
PRIMER_LEFT_2_PENALTY=0.225996
PRIMER_RIGHT_2_PENALTY=0.088755
PRIMER_LEFT_2_SEQUENCE=TTGTCGGTGGAACTGAAGTAGA
PRIMER_RIGHT_2_SEQUENCE=TTAATTTAGTCCTCGCCTGGAA
PRIMER_LEFT_2=116,22
PRIMER_RIGHT_2=354,22
PRIMER_LEFT_2_TM=59.774
PRIMER_RIGHT_2_TM=60.089
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.3389
PRIMER_LEFT_3_PENALTY=0.171757
PRIMER_RIGHT_3_PENALTY=0.167146
PRIMER_LEFT_3_SEQUENCE=ATGAACAAGGTTGAAAGACGCT
PRIMER_RIGHT_3_SEQUENCE=CTTTTCCTTCTCCTTCTCCCTC
PRIMER_LEFT_3=95,22
PRIMER_RIGHT_3=275,22
PRIMER_LEFT_3_TM=60.172
PRIMER_RIGHT_3_TM=59.833
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.3389
PRIMER_LEFT_4_PENALTY=0.171757
PRIMER_RIGHT_4_PENALTY=0.167146
PRIMER_LEFT_4_SEQUENCE=ATGAACAAGGTTGAAAGACGCT
PRIMER_RIGHT_4_SEQUENCE=CTCTTTTCCTTCTCCTTCTCCC
PRIMER_LEFT_4=95,22
PRIMER_RIGHT_4=277,22
PRIMER_LEFT_4_TM=60.172
PRIMER_RIGHT_4_TM=59.833
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_00163 Ps_contig_mira-and-tgicl-ass_franssen_2053
SEQUENCE=CAAGGCAGCATGCAAATAGCAAATAATNACGATTATTGCTGATTGGTAACAAAGTAGTGANCTTGCTCACANACGGGCTGTATGCACAGAAGAAGGCGAACAGTTTGCAAAGGAGCATGGGTTGATCTTCATGGAGGTCCTTCAGCAAAAACTGCTCAGAATGTTGAAGAGGCCTTCATAAAAACAGCTGGAACCATATACAAAAAGATTCAGGATGGAGTTTTTGATGTATCAAATGAGTCTTATGGAATAAAAGTAGGATATGGTGGAATTCCTGGACCATCGTGGGGGTAGGGATGGCCCTTCTGCTGCTGGTGGAAGCTGCTGCAGTTGAAGTGAAACATATCGCAACCTTGTTTGTTACGAACGTTGTTTCTTTATGTATTCTTCTTCTTCGTTTTGGGTTGGATTATTAGTGGGTAAAGTTATACCTAACG
TARGET=385,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0902
PRIMER_LEFT_PENALTY=0.033905
PRIMER_RIGHT_PENALTY=0.056343
PRIMER_LEFT_SEQUENCE=TAGGATATGGTGGAATTCCTGG
PRIMER_RIGHT_SEQUENCE=CCCACTAATAATCCAACCCAAA
PRIMER_LEFT=256,22
PRIMER_RIGHT=419,22
PRIMER_LEFT_TM=60.034
PRIMER_RIGHT_TM=59.944
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.2497
PRIMER_LEFT_1_PENALTY=0.193337
PRIMER_RIGHT_1_PENALTY=0.056343
PRIMER_LEFT_1_SEQUENCE=CTTCAGCAAAAACTGCTCAGAA
PRIMER_RIGHT_1_SEQUENCE=CCCACTAATAATCCAACCCAAA
PRIMER_LEFT_1=139,22
PRIMER_RIGHT_1=419,22
PRIMER_LEFT_1_TM=59.807
PRIMER_RIGHT_1_TM=59.944
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.4941
PRIMER_LEFT_2_PENALTY=0.437791
PRIMER_RIGHT_2_PENALTY=0.056343
PRIMER_LEFT_2_SEQUENCE=CAGCAAAAACTGCTCAGAATGT
PRIMER_RIGHT_2_SEQUENCE=CCCACTAATAATCCAACCCAAA
PRIMER_LEFT_2=142,22
PRIMER_RIGHT_2=419,22
PRIMER_LEFT_2_TM=59.562
PRIMER_RIGHT_2_TM=59.944
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.5536
PRIMER_LEFT_3_PENALTY=0.033905
PRIMER_RIGHT_3_PENALTY=0.519744
PRIMER_LEFT_3_SEQUENCE=TAGGATATGGTGGAATTCCTGG
PRIMER_RIGHT_3_SEQUENCE=ACCCACTAATAATCCAACCCAA
PRIMER_LEFT_3=256,22
PRIMER_RIGHT_3=420,22
PRIMER_LEFT_3_TM=60.034
PRIMER_RIGHT_3_TM=59.480
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.6110
PRIMER_LEFT_4_PENALTY=0.554687
PRIMER_RIGHT_4_PENALTY=0.056343
PRIMER_LEFT_4_SEQUENCE=TCAGCAAAAACTGCTCAGAATG
PRIMER_RIGHT_4_SEQUENCE=CCCACTAATAATCCAACCCAAA
PRIMER_LEFT_4=141,22
PRIMER_RIGHT_4=419,22
PRIMER_LEFT_4_TM=60.555
PRIMER_RIGHT_4_TM=59.944
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=279
=
PRIMER_SEQUENCE_ID=SSR_00164 Ps_contig_mira-and-tgicl-ass_franssen_2054
SEQUENCE=AGTCATCCTACGCACCGAAACCTACCAACACCCAACTAGGTCAGGTCATGAACAGGTTGAAAGCGCTTGTTTCGGTTGAACTGAAGTAGAAGACATGACTTTCCAACTCCAGTGTCTCCGATGATGATATACTTGAAGAGATATGCGTAAGACATTTGTTGTGNGCGAGAGAGAACGAGAAGGAAGAGGAAAGAAAGAAAAGCGAAGGAAGGGGAAAAGGAGAAAGGAAAAGAGAGAAGAAG
TARGET=189,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0622
PRIMER_LEFT_PENALTY=0.012470
PRIMER_RIGHT_PENALTY=0.049761
PRIMER_LEFT_SEQUENCE=GTCAGGTCATGAACAGGTTGAA
PRIMER_RIGHT_SEQUENCE=TCTTTTCCTTTCTCCTTTTCCC
PRIMER_LEFT=39,22
PRIMER_RIGHT=232,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.050
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=0.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=0.2609
PRIMER_LEFT_1_PENALTY=0.211105
PRIMER_RIGHT_1_PENALTY=0.049761
PRIMER_LEFT_1_SEQUENCE=CCTACCAACACCCAACTAGGTC
PRIMER_RIGHT_1_SEQUENCE=TCTTTTCCTTTCTCCTTTTCCC
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=59.789
PRIMER_RIGHT_1_TM=60.050
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.2653
PRIMER_LEFT_2_PENALTY=0.215550
PRIMER_RIGHT_2_PENALTY=0.049761
PRIMER_LEFT_2_SEQUENCE=ACACCCAACTAGGTCAGGTCAT
PRIMER_RIGHT_2_SEQUENCE=TCTTTTCCTTTCTCCTTTTCCC
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=232,22
PRIMER_LEFT_2_TM=59.784
PRIMER_RIGHT_2_TM=60.050
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.4046
PRIMER_LEFT_3_PENALTY=0.354881
PRIMER_RIGHT_3_PENALTY=0.049761
PRIMER_LEFT_3_SEQUENCE=ACTTTCCAACTCCAGTGTCTCC
PRIMER_RIGHT_3_SEQUENCE=TCTTTTCCTTTCTCCTTTTCCC
PRIMER_LEFT_3=97,22
PRIMER_RIGHT_3=232,22
PRIMER_LEFT_3_TM=59.645
PRIMER_RIGHT_3_TM=60.050
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=0.5324
PRIMER_LEFT_4_PENALTY=0.482662
PRIMER_RIGHT_4_PENALTY=0.049761
PRIMER_LEFT_4_SEQUENCE=TCCAGTGTCTCCGATGATGATA
PRIMER_RIGHT_4_SEQUENCE=TCTTTTCCTTTCTCCTTTTCCC
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=232,22
PRIMER_LEFT_4_TM=60.483
PRIMER_RIGHT_4_TM=60.050
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_00165 Ps_contig_mira-and-tgicl-ass_franssen_2122
SEQUENCE=GTGGGGGGATTCATTTTTCTCTCTCTCTCAAAAGCTTTTCACTTGAATTGAGTTGATGATGGCTTCCGAATCTGAAGTTTCTGAACTTTCTTATATCTTCGAAAGAATGATCAGAAACAGAGACATGTCTCTGTTGTTGCCCTTCATCCAAGGTCTTTCCGAATCTTCAATTCAAAGACACAGGGACAACCCAGTTCAAGAATCTTCGAGCAACGAAGAAGATTCCAACCGTCAAAGAATCATCTTGGTGAACCCTTCTACACAGCGCATGATCGTGATCGAAGGAGTTTCAAGTCTGGAAACTCTGTTTCAAGGAAACTTAGAAAAGTAACTAGAAAGAAAGGTCAGCCACCAGCTTCAA
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00166 Ps_contig_mira-and-tgicl-ass_franssen_2127
SEQUENCE=ATGAGGAAGGCCTAGGTCAAAGTACTTGATTGTCCAACTCCAAGAAAGGAAAAATGAGCTTATCCTTGATTGACTTCCAGATAACTCGGGTCATTTCATCTCCGTCCATCTCAACGATGGGACTGGCGACTTTGATTTTCNGGAATGCCATGGTGGTGATCGGAAGAAGAGAAGAGGATGAAGATGCGGGARGAGAGTGKTAGGTKARGAGAAAGTAAGTAGGACGGACGGACGGACCGGTACCCGGTACCGTAGTTACCTCGGTCGGTTACGGTTACGTAACCCTACCGTTCGG
TARGET=222,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00167 Ps_contig_mira-and-tgicl-ass_franssen_2135
SEQUENCE=TYWCTTGGCGATCCGGAACCTAGTTGTAGCAGCTTGTGTCTGCAATTGAGAGCTCAAGTCCTCTATTTTTGTCTCCCCAAATATAACGTAGGTCTCAGAGTTTGGACTCTTGAAGACATCAGGTTTTGAGATGAAAAACATTATATTTTTTGTTCTCTTGATTGTTACCCTGCTAACACCATCAATTGGTTTTAGTCCCAGCTTCAACATTGCTTTGCGACTCTTCTTCTCACTTCTRCTCTGWCKTTGATACCTWCWGCTWCCACCTTGAGCGGCGTCATCTTCCTCGTCATCGGATTCGTCTTTCTCGTCGTCGTCGTCATCCTCGACAATAGGAGCGTTCGTTTTTCTTCCGTGGGGAANATGTCGTCCTTTCGTTGGGTAGGGGTTTTCGTTTTACCAGTCCGGACGGGAGGGGTAGTTTGGTTCGGGACGGGTCCGGGTTTCCGGTCGGGTCGGTT
TARGET=308,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00168 Ps_contig_mira-and-tgicl-ass_franssen_2156
SEQUENCE=CTTTGCCACTGAGGTGAGGTTTACAGAAGGCATTGAACCAGAAGCTGGTTCGGTACTGTGGAAGTAGAAGAAAAGTTAGAAGAAGAGAAGAGTGTGAGTTTTCAGGAGAGAGAGAGAGAG
TARGET=106,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00169 Ps_contig_mira-and-tgicl-ass_franssen_2161
SEQUENCE=GTTCGGGGTTGGCGCGTGCGTTTTGTTGKATATAAAAAGACAATGATGTATCAGACGAARTGGAGGGATTTTCTTCGAAATCTAATTCTTCTTCTTCTTCTAGTTCTAGATTCCGACTCGCCGTGTTGACTCGGTCCGGGTCGGGGTTTTACTACGACTCATTTCTGAATCTTGATTTTCAATTCTCACGCGGATCTAACTCCTCCACAACCGAGGAAAACAAAGGACTTATCACAATGAAGGCATTGATTCTGGTTGGAGGGTTTGGAACTAGGTTGAGGCCATTGACACTCAGTGTCCCTAAGCCTCTTGTTGATTTTGAACTAACAAACTCATGATTTTGCATCA
TARGET=86,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00170 Ps_contig_mira-and-tgicl-ass_franssen_2237
SEQUENCE=GTAACATGAAAATTGAAATGATGCAAATAAGCATAACACAAATAAGAAACAATATACAAACTTAGTAGTGGAGGGGTAATTTAAAAGAATTWCATCTAAATCATACTCAACCGAGAAAWTTACAAAAACTCCCCTCGTDGAAATCCACGCCTTCAATTTGCTCAATTTCATCCTCCCTCATAAACTCTCTCAACCTCTCCTTAAAAATCCCAACTGCTCCTCCAAAAAGCTCTTCTGAGAATCAAGTTCAACAACATTCCACCTCAACCGACTACAACTACCTACCTTACCCGTACTACTACTACTTCTTCTCCTCCTCCTCCACCACCTACCCTACCCGTAACCTACGTACTTCCGTCCTACCTAACTACCTACCTAACCCGTAACCTTACCTTT
TARGET=294,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00171 Ps_contig_mira-and-tgicl-ass_franssen_2237
SEQUENCE=GTAACATGAAAATTGAAATGATGCAAATAAGCATAACACAAATAAGAAACAATATACAAACTTAGTAGTGGAGGGGTAATTTAAAAGAATTWCATCTAAATCATACTCAACCGAGAAAWTTACAAAAACTCCCCTCGTDGAAATCCACGCCTTCAATTTGCTCAATTTCATCCTCCCTCATAAACTCTCTCAACCTCTCCTTAAAAATCCCAACTGCTCCTCCAAAAAGCTCTTCTGAGAATCAAGTTCAACAACATTCCACCTCAACCGACTACAACTACCTACCTTACCCGTACTACTACTACTTCTTCTCCTCCTCCTCCACCACCTACCCTACCCGTAACCTACGTACTTCCGTCCTACCTAACTACCTACCTAACCCGTAACCTTACCTTT
TARGET=311,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00172 Ps_contig_mira-and-tgicl-ass_franssen_2262
SEQUENCE=CCGTTTTAGAACAARACAAAACATTAGCCAAGAGCAGCAAAATATTTGGTAGCAAGACATTTTATTCATAAAATAGACAATCATCTACATAAGATGATCAAATCATCAGCATAGCATCCTACATAAATGTTATCATCATCCAATTTTCTACTCGTCATCATCCTCCTCCTCACTTTCTTCCTCATCATCATTCTCATCATGCACCTCAGATTCTGATTTCTCGGATTCTTCTTCTTCTTCAACAGCAGCTGGACCTTCAGCTTGTTTCTTGTTATAGGACTTCATGCTCTTCGCATATTCAGCCTTCCTTTTCTCTGCCTTAGCAGCATATGGTGCTTTCTCAGCTTCAGTCATTGACTTCCTATTTTCGACCCCGCAGCTTTGCCCACTGCAGACACAGCTTTGTTGTCTAGGGTTTTCCTTTTGACTGCTTCCTAAAGTCCTCCATAGAAAACGAAGAAAGCACTTGGAGGTCTCTTCGGCTTGGTTACGGGTTCCCTTTTTCGGGTTTTCCCCTTTTTCCCCCGTTTGGGGTCCTTTCGGGGTTTTTCCCTTTTCCCCGGTCCCCGTTTTTGGGTCGGGGCCGGGGGGTAACCGTCCCCTTAACCCCCCTTTTTTCCGTTTTAAACCTTTTCCCCGAAACCCCCGGGTACCTTAAAACGGGTTTCCCCGTTTTTTTCCCGGGGTTTTTTTACCCTAAACGGGTTTTCCCCTTTTTTTTT
TARGET=226,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00173 Ps_contig_mira-and-tgicl-ass_franssen_2264
SEQUENCE=GAAAAGGAAGGCTGAATATGAGAAGAGCATGAAGTCCTATAACAAGAAACAAGCTGAAGGTCCAGCTGCTGTTGAAGAAGAAGAAGAATCCGAGAAATCAGAATCTGAGGTGCATGATGAGAATGATGATGAGGAAGAAAGTGAGGAGGATGATGACGAGTAGAAAATTGGATGATGATAACATTTATGTAGGATGCTATGCTGATGATTTGATCATCTTATGTAGATGATTGTCATTTTATGATAAAAATGTCTTGCTACCAATATTTGCTGCTCTTGGCTTAATTGTTTTGTCTGTTACAAAACGTGAAATAAGTTAAAATGGAGGGAGTACTTAAACTTTTTATTTGTACTTTAACGGCCTAACTTCTTTAAGTTAAGAGTATTTGTTTTATTCGTTTTAGGTAGTAGTTCTTTTAGTATTTTCA
TARGET=74,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2721
PRIMER_LEFT_PENALTY=0.205226
PRIMER_RIGHT_PENALTY=0.066849
PRIMER_LEFT_SEQUENCE=ACAAGAAACAAGCTGAAGGTCC
PRIMER_RIGHT_SEQUENCE=TCAAATCATCAGCATAGCATCC
PRIMER_LEFT=41,22
PRIMER_RIGHT=212,22
PRIMER_LEFT_TM=59.795
PRIMER_RIGHT_TM=60.067
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.2807
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.075444
PRIMER_LEFT_1_SEQUENCE=ACAAGAAACAAGCTGAAGGTCC
PRIMER_RIGHT_1_SEQUENCE=AAACAATTAAGCCAAGAGCAGC
PRIMER_LEFT_1=41,22
PRIMER_RIGHT_1=290,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=59.925
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.3460
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.140792
PRIMER_LEFT_2_SEQUENCE=ACAAGAAACAAGCTGAAGGTCC
PRIMER_RIGHT_2_SEQUENCE=ACTCGTCATCATCCTCCTCACT
PRIMER_LEFT_2=41,22
PRIMER_RIGHT_2=160,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=60.141
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=0.8543
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.649076
PRIMER_LEFT_3_SEQUENCE=ACAAGAAACAAGCTGAAGGTCC
PRIMER_RIGHT_3_SEQUENCE=CTCGTCATCATCCTCCTCACTT
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=159,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=60.649
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=0.8825
PRIMER_LEFT_4_PENALTY=0.205226
PRIMER_RIGHT_4_PENALTY=0.677241
PRIMER_LEFT_4_SEQUENCE=ACAAGAAACAAGCTGAAGGTCC
PRIMER_RIGHT_4_SEQUENCE=CCTCCTCACTTTCTTCCTCATC
PRIMER_LEFT_4=41,22
PRIMER_RIGHT_4=148,22
PRIMER_LEFT_4_TM=59.795
PRIMER_RIGHT_4_TM=59.323
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_00174 Ps_contig_mira-and-tgicl-ass_franssen_2265
SEQUENCE=CTCTTCACTCGGTTCATCATGAAGGAGGAAAGTCCAAGGTGAATCGAGAAAGCTGAACGAAGCTTGCGGTGAATAAGAAGGGTGCTCCCGCCACAAAGGGCGGAAAGAAACCAGCCAAGGGAAAGGAACCAAAGGACCCTAACAAGCCGAAGAGACCTCCAAGTGCTTTCTTCGTTTTCATTGGTAGGTACTTTTAGGTAAGCAGTTCAAAAAGGAAAACCCTGACAACAAAGCTGTGTCTGCAGTGGGCAAAGCTGCTGGGGCAAAATGGAGGTCAATGACTGAAGCTGAGAAAGCACCATATGCTGCTAAGGCAGAGAAAGGAGCTGAATATGAGAGAGCATGAAGTCCTATAACAAGAAACAAGCTGGAACGGTCCAGTCGTGGTCGTTGTTAGGAAAGAAAGAAA
TARGET=398,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_00175 Ps_contig_mira-and-tgicl-ass_franssen_2267
SEQUENCE=TCGGGGGTAAACTASACACAAACAAACAATCACATAACTAGAAAATGGAGAGCACACAGAGAAGTTTAAGCTTGTTGGTACTTTGTTTCCTGCTTATGGGAACAACAATGTTAGTGAGTGGTCAGAGTGCAAACAACGTAAGAGCAACATACAATAACTACAACCCTCAGAACATAATGGATTACAACAGNGCCNGTGTGTACTGTGCCTACCGTGGGATGACCTAAACCGACGTTCC
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00176 Ps_contig_mira-and-tgicl-ass_franssen_2302
SEQUENCE=AAGACAGTGGGTATCAACGCAGAGTCAAAAAATTTATGAGTTGAGGGTGCTTTTTCACTTCTGACCTCCCTCCCTCCAAGAGAAAATCAATAGCTGTTGATGTGGGGGAAATATGTGACTATGGTTCTTTTCTTACACCGACTACCTTCAAAGTAAAAGTATTTTGAAAGGAAAGGAAAGATAAACCTCTCTTGATGAGGTGATACACTGTTCTCATTTTGTGCAGAGAGAGTCACGAGAAGTTTAGATGAGAGCTTGGAAAGGTTGCAG
TARGET=65,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2656
PRIMER_LEFT_PENALTY=0.151106
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=GTTGAGGGTGCTTTTTCACTTC
PRIMER_RIGHT_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT=39,22
PRIMER_RIGHT=266,22
PRIMER_LEFT_TM=60.151
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=0.3197
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.114470
PRIMER_LEFT_1_SEQUENCE=AGGGTGCTTTTTCACTTCTGAC
PRIMER_RIGHT_1_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_1=43,22
PRIMER_RIGHT_1=266,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.3197
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=GAGTTGAGGGTGCTTTTTCACT
PRIMER_RIGHT_2_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=266,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.3892
PRIMER_LEFT_3_PENALTY=0.274768
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=TTGAGGGTGCTTTTTCACTTCT
PRIMER_RIGHT_3_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=60.275
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.6076
PRIMER_LEFT_4_PENALTY=0.151106
PRIMER_RIGHT_4_PENALTY=0.456523
PRIMER_LEFT_4_SEQUENCE=GTTGAGGGTGCTTTTTCACTTC
PRIMER_RIGHT_4_SEQUENCE=CGTGACTCTCTCTGCACAAAAT
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=236,22
PRIMER_LEFT_4_TM=60.151
PRIMER_RIGHT_4_TM=59.543
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=198
=
PRIMER_SEQUENCE_ID=SSR_00177 Ps_contig_mira-and-tgicl-ass_franssen_2302
SEQUENCE=AAGACAGTGGGTATCAACGCAGAGTCAAAAAATTTATGAGTTGAGGGTGCTTTTTCACTTCTGACCTCCCTCCCTCCAAGAGAAAATCAATAGCTGTTGATGTGGGGGAAATATGTGACTATGGTTCTTTTCTTACACCGACTACCTTCAAAGTAAAAGTATTTTGAAAGGAAAGGAAAGATAAACCTCTCTTGATGAGGTGATACACTGTTCTCATTTTGTGCAGAGAGAGTCACGAGAAGTTTAGATGAGAGCTTGGAAAGGTTGCAG
TARGET=166,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2656
PRIMER_LEFT_PENALTY=0.151106
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=GTTGAGGGTGCTTTTTCACTTC
PRIMER_RIGHT_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT=39,22
PRIMER_RIGHT=266,22
PRIMER_LEFT_TM=60.151
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=0.3197
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.114470
PRIMER_LEFT_1_SEQUENCE=AGGGTGCTTTTTCACTTCTGAC
PRIMER_RIGHT_1_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_1=43,22
PRIMER_RIGHT_1=266,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.3197
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=GAGTTGAGGGTGCTTTTTCACT
PRIMER_RIGHT_2_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=266,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.3197
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=GGTGCTTTTTCACTTCTGACCT
PRIMER_RIGHT_3_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.3852
PRIMER_LEFT_4_PENALTY=0.270774
PRIMER_RIGHT_4_PENALTY=0.114470
PRIMER_LEFT_4_SEQUENCE=TTTTTCACTTCTGACCTCCCTC
PRIMER_RIGHT_4_SEQUENCE=CAACCTTTCCAAGCTCTCATCT
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=59.729
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_00178 Ps_contig_mira-and-tgicl-ass_franssen_2305
SEQUENCE=GGGTCTTAGTCACGTTTCTTCAATGTGGACTCTGTACTAAGCATACTCACTGAGAGAAAGAGAGAGAGGAGAGAGAGAGAGAATGTCYTMCCCAAGCAAACGCCGAGACATGGATCTGATAGAAGCTGATGATGGCTAGAATTTAACGAAAAGGTGGGTAAGTAATTTAATACGAAATTGGAACTTAACCCAAGTTCGGAACCGAAAAGGTAACCGGTACCTTTTTCCCGGTTTTTTTTACGGGGTTAAACCCGGTTTAAAAACGTTTTTTTAACCCCCGGAAAACCCCGGGGGGTTTAAAAAACCCCCTTAAAAAAAACGGGTTAAACCCCCGG
TARGET=59,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00179 Ps_contig_mira-and-tgicl-ass_franssen_2305
SEQUENCE=GGGTCTTAGTCACGTTTCTTCAATGTGGACTCTGTACTAAGCATACTCACTGAGAGAAAGAGAGAGAGGAGAGAGAGAGAGAATGTCYTMCCCAAGCAAACGCCGAGACATGGATCTGATAGAAGCTGATGATGGCTAGAATTTAACGAAAAGGTGGGTAAGTAATTTAATACGAAATTGGAACTTAACCCAAGTTCGGAACCGAAAAGGTAACCGGTACCTTTTTCCCGGTTTTTTTTACGGGGTTAAACCCGGTTTAAAAACGTTTTTTTAACCCCCGGAAAACCCCGGGGGGTTTAAAAAACCCCCTTAAAAAAAACGGGTTAAACCCCCGG
TARGET=69,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00180 Ps_contig_mira-and-tgicl-ass_franssen_2324
SEQUENCE=GATTCTTATTTTTTCTATCACCGCTTCAAAAATCAGKAGCATTGCAATCATCATCATCATCCATGGCTCCCAAAAGGGTTCTTCTCCTTTGCGGTGACTTCATGGAAGACTATGAAGCTATGGTTCCTTTTCNGGCATTGCAAGCATTCGGTATCACCGTCGATGCTGTTTCGTACCCGGTAAAAATCCGGTGAAGTCTGCCGCCACCCGCCGTCCATATGTTCCG
TARGET=47,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00181 Ps_contig_mira-and-tgicl-ass_franssen_2341
SEQUENCE=CAAAACCTCAAGACTGATTGGAAGAAGGTGGTTTCCGTTAGTTCAACATCATATTCCTGATTTTTCTGGTCATTGTATACTCCATCGGCTGCTGCGCATTCAGGAATAACAGGCAGGACAATTGGAAGAGATATTAGGCTCTTGAGCATTATTAGCATGTAGATGTATTTAGGCTATATTGGTTTATATGCTGTATTTGTGTGTGTGTATTCTATTACTTTGTTTTACAAGTTACTAGTTTGTACTTTGTTTAGTTAGTTAAATTGTACTTAATTATAGTATANAGTNTGTGTATTAGAATTAACGTAAAACTTAAAAAAACT
TARGET=198,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00182 Ps_contig_mira-and-tgicl-ass_franssen_2342
SEQUENCE=GGCTGCTGCGCATTCAGGAATAACAGGCAGGACAATTGGAAGAGATATTAGGCTCTTGAGCATTATTAGCATGTAGATGATTTAGGCWATATGGTTTATATGCTGTATTTTGTGTGTGTGGATTTCATTACTTGTTTTTCAGTACTAGTTTGACTTGTTTGTAGTAAATTGACTTAAACTTTATAGTATTACAGTATGTGGTATTAGWAATTGAWACTTACWAAAYKTAACCGTTTGGTGACTAACTTACCTTAAGTTAGTTTAAACTAAAACCAAAAAAAAAAC
TARGET=111,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00183 Ps_contig_mira-and-tgicl-ass_franssen_2343
SEQUENCE=GATCTTTCTCAACAATCAAAACAACCATTTCAATTTTTCCATTACTATAAAACAACATTTTCTCTTTCACAAAAAAAAAACCCTAAAACCCATTTCATCTTCATCTTCATCTTCATCTATCAATTCAATTCAATTCAACCATGTCTCGATTCAGTAACAACCTAATCGGAATCCTCAATTTCCTAACCCTACTCCTCTCAATCCCAATTCTAGTCATGGGAGTATGGCTCCACAAACAGTCCACCACGGAATGCGAACGCTTCTTAGACGAAACCCATTATTCGCCCTTTGGKCGTTTTCCTCTTGGTTGTTTCT
TARGET=120,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00184 Ps_contig_mira-and-tgicl-ass_franssen_2346
SEQUENCE=TAAGTGGGTGGTGGTTGTTGATGTTGTTGTAGACAAAGGAATGAGCATCATGTTTTTTCTATATGTTTGACCCAACATGTGAGAATGCTAAAAATTTGCTTAATCAAGTTGTTTCCTTCATCAAAAGAACTTGAAAATATGAGGGTGGGAGTGATGCTTCCTTTATTTTGTTTTCTATGTTCTATTATCATTTTAAATATTTTTATGTACTCTTTCTTGTGTGTTAAAGAAAAAAATCCAAAAAGTTAATAAAATAAAATAAATTTTTAACATAAGATTTATGRTT
TARGET=248,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00185 Ps_contig_mira-and-tgicl-ass_franssen_2349
SEQUENCE=CAACGCAGAGTACGCGGGATACCAACACAAACACAAACCAATTCCTACTGTATCTCAATACAAAAAATGGCCACACTATTTTCATCTTCATCTCTTCATTCAATCCGTCTTTCTTCTTCTACATTCCCCGTTTCTTCCTCTTCTTCTCAATCCCTTCAATTTTGCNCTTTTCCATGCCACCTCGTTGAGCNCTGTCAATAAAAAAGTAACCGTTGTTTTACTTGTTGGTTAAGGCAGTGGAAGATAGAAACCCAACCAAACAAACAAAGAAACG
TARGET=257,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00186 Ps_contig_mira-and-tgicl-ass_franssen_2384
SEQUENCE=GGGTATTGATTCAAGATTAGATGAAAGTTTACATGATGTTTTAGTAATGAATTGTATCAATTCATTTCAATTTGACAAGTCAAGCTTATTTGTATCAAGTATTAAATTGCACATGGTATTTGGACAAAGTGAGTCTTATTTGTATAATGTAATGTAATTAGTTAAATTTGGTTTTATTTCTTATTTTTGAAGACAAGTAACTTCACAATAACTAACTAAAACTATTTATTTATTACTATTAACTAACCAAAAC
TARGET=223,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00187 Ps_contig_mira-and-tgicl-ass_franssen_2396
SEQUENCE=AACGTTTWCGGGGATAACAACTTAACTAACCATTGACACTTAGAGCATCCATCCATCATGTCGCTCGCTTCCATTTCTTCGGCATTCTCCTCATTCTCAATTTCATTACCCTCCTCCTCCCGTTCTACTTCACTTTCATTTCTAACTTACTAATTTCCTAAGAAATACCCTCCTTCTCATCCTCAATTAGCAGCAACATGATAGACCAAAAAGACGAGTGGTTGT
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00188 Ps_contig_mira-and-tgicl-ass_franssen_2399
SEQUENCE=GGAAATCCAAAATCCCCAAACCACACCCAACCATTCTTCCATTGGTCTTCACGTCCATTCATTTCCACCTCACATCACTACAATAACCTCAAAAAACTTCCTTCATCCACTTCACTCTCTCACTCTCCCTCTCTCTCTCTCTACAATGGCTTCAGCTACAGCACCCACAACTCTCTCCCTCCTCCCCACCGCAAAATCCTCCACCTCATCAGCAACCCCATCTTCCTCCCGTGTCCACCTCTCCTCCTCATTCCTTGGCGCCCGTCCGCTCCGCCGACTAGGTTTCTCGGCTGCCGATCCCTTGCTGGCATTGCGAGGTGGCGTCCAAGGTGAGGACAGTGAGGG
TARGET=129,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2053
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=0.984274
PRIMER_LEFT_SEQUENCE=CAACCATTCTTCCATTGGTCTT
PRIMER_RIGHT_SEQUENCE=GAGAGAGTTGTGGGTGCTGTAG
PRIMER_LEFT=27,22
PRIMER_RIGHT=176,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=59.016
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=150
PRIMER_PAIR_PENALTY_1=1.2866
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=1.065587
PRIMER_LEFT_1_SEQUENCE=CAACCATTCTTCCATTGGTCTT
PRIMER_RIGHT_1_SEQUENCE=GAATGAGGAGGAGAGGTGGAC
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=252,21
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=60.066
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=57.143
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=1.5359
PRIMER_LEFT_2_PENALTY=0.220992
PRIMER_RIGHT_2_PENALTY=1.314939
PRIMER_LEFT_2_SEQUENCE=CAACCATTCTTCCATTGGTCTT
PRIMER_RIGHT_2_SEQUENCE=AAGGAATGAGGAGGAGAGGTG
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=255,21
PRIMER_LEFT_2_TM=60.221
PRIMER_RIGHT_2_TM=59.685
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=1.5359
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=1.314939
PRIMER_LEFT_3_SEQUENCE=CAACCATTCTTCCATTGGTCTT
PRIMER_RIGHT_3_SEQUENCE=CAAGGAATGAGGAGGAGAGGT
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=256,21
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.685
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=1.6033
PRIMER_LEFT_4_PENALTY=0.619003
PRIMER_RIGHT_4_PENALTY=0.984274
PRIMER_LEFT_4_SEQUENCE=ATTGGTCTTCACGTCCATTCAT
PRIMER_RIGHT_4_SEQUENCE=GAGAGAGTTGTGGGTGCTGTAG
PRIMER_LEFT_4=40,22
PRIMER_RIGHT_4=176,22
PRIMER_LEFT_4_TM=60.619
PRIMER_RIGHT_4_TM=59.016
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_00189 Ps_contig_mira-and-tgicl-ass_franssen_2405
SEQUENCE=CCCGTTTGTCGTGTCTCAGCATCAATACTGAAGAAAAATAAGACAGAATTTTTAGTTTAACCTTACCCAACTTAGATAACCGAACCGGAAAACCTAGCTACGAAAACCGAACGCTCCTCCCAATAACACCCCTCCCAACCAACCAAAACCTACCTACTCCGTCAACAACACCATCAACAACACCATCAACCACTCCATCAACCCCATCTTCTGAGACAAATACAACTTCTCCACCACCACCAAAGGATAGTGGAGCTGTCGGTGTTTCTAATGGTGTTGGTATTTTGATGGGATCTTTGTTTTTGGCTTTGGGTTTCATGGGCTAGGGAAGAGACAGTGCAATGATTATTATTGATCATTTTAATTTTATGTGTTATTGGTTTTTGTTGAATTTGGTTTAATTTTATTTAATGATTTGAGATGTACATCTTATCTTCATCTTGATATGTATCTATTTTAATGAAAAAAACTTCATTTWAAAATTT
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00190 Ps_contig_mira-and-tgicl-ass_franssen_2405
SEQUENCE=CCCGTTTGTCGTGTCTCAGCATCAATACTGAAGAAAAATAAGACAGAATTTTTAGTTTAACCTTACCCAACTTAGATAACCGAACCGGAAAACCTAGCTACGAAAACCGAACGCTCCTCCCAATAACACCCCTCCCAACCAACCAAAACCTACCTACTCCGTCAACAACACCATCAACAACACCATCAACCACTCCATCAACCCCATCTTCTGAGACAAATACAACTTCTCCACCACCACCAAAGGATAGTGGAGCTGTCGGTGTTTCTAATGGTGTTGGTATTTTGATGGGATCTTTGTTTTTGGCTTTGGGTTTCATGGGCTAGGGAAGAGACAGTGCAATGATTATTATTGATCATTTTAATTTTATGTGTTATTGGTTTTTGTTGAATTTGGTTTAATTTTATTTAATGATTTGAGATGTACATCTTATCTTCATCTTGATATGTATCTATTTTAATGAAAAAAACTTCATTTWAAAATTT
TARGET=231,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00191 Ps_contig_mira-and-tgicl-ass_franssen_2407
SEQUENCE=AAAAGAATGTGTTTGGTAAGAAAGAGGATTCAAGTTGGGAAGAAGAGAACAATAGTGGTTTTAGTTTGTGGAAATGGAGTCTTAGTGGTGTTGGTGCTATTTGTACTTTTGGTGTTGTTGCTGCTTCTAATTAGGAAGAAGAAGAAAAAGAGAGTGAATAGAAAACAAGAATGATCGAATTCGAATGGGACCCCGCGTACTCTGTCGTTTGTATTTACCTTTTACTTTGTTTTTTCTT
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.9765
PRIMER_LEFT_PENALTY=0.065960
PRIMER_RIGHT_PENALTY=4.910554
PRIMER_LEFT_SEQUENCE=TGGAGTCTTAGTGGTGTTGGTG
PRIMER_RIGHT_SEQUENCE=GGTAAATACAAACGACAGAGTACG
PRIMER_LEFT=74,22
PRIMER_RIGHT=218,24
PRIMER_LEFT_TM=60.066
PRIMER_RIGHT_TM=57.089
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=5.0478
PRIMER_LEFT_1_PENALTY=0.065960
PRIMER_RIGHT_1_PENALTY=4.981798
PRIMER_LEFT_1_SEQUENCE=TGGAGTCTTAGTGGTGTTGGTG
PRIMER_RIGHT_1_SEQUENCE=AGGTAAATACAAACGACAGAGTACG
PRIMER_LEFT_1=74,22
PRIMER_RIGHT_1=219,25
PRIMER_LEFT_1_TM=60.066
PRIMER_RIGHT_1_TM=58.018
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=5.0604
PRIMER_LEFT_2_PENALTY=0.149895
PRIMER_RIGHT_2_PENALTY=4.910554
PRIMER_LEFT_2_SEQUENCE=TACTTTTGGTGTTGTTGCTGCT
PRIMER_RIGHT_2_SEQUENCE=GGTAAATACAAACGACAGAGTACG
PRIMER_LEFT_2=103,22
PRIMER_RIGHT_2=218,24
PRIMER_LEFT_2_TM=59.850
PRIMER_RIGHT_2_TM=57.089
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=5.0979
PRIMER_LEFT_3_PENALTY=0.187326
PRIMER_RIGHT_3_PENALTY=4.910554
PRIMER_LEFT_3_SEQUENCE=TGGTGTTGTTGCTGCTTCTAAT
PRIMER_RIGHT_3_SEQUENCE=GGTAAATACAAACGACAGAGTACG
PRIMER_LEFT_3=109,22
PRIMER_RIGHT_3=218,24
PRIMER_LEFT_3_TM=59.813
PRIMER_RIGHT_3_TM=57.089
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=110
PRIMER_PAIR_PENALTY_4=5.1317
PRIMER_LEFT_4_PENALTY=0.149895
PRIMER_RIGHT_4_PENALTY=4.981798
PRIMER_LEFT_4_SEQUENCE=TACTTTTGGTGTTGTTGCTGCT
PRIMER_RIGHT_4_SEQUENCE=AGGTAAATACAAACGACAGAGTACG
PRIMER_LEFT_4=103,22
PRIMER_RIGHT_4=219,25
PRIMER_LEFT_4_TM=59.850
PRIMER_RIGHT_4_TM=58.018
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=117
=
PRIMER_SEQUENCE_ID=SSR_00192 Ps_contig_mira-and-tgicl-ass_franssen_2408
SEQUENCE=TATGTGATTCGAGAGTGAGAATGCTCAATGAATCGAGATAGAGGGTGTGNGAAAGATGTGTTTGGTAAGAAAGAGGATTCAATTGGAAGAAGAGAACAATAGTGGTTTTAGTTTGTTGGTAAGTAGGAGKYGTTAGTTGGTTGGTTTGGGTTGGTCGTATTTWGTTTACGTTTTGGTTTGGTTTGGTTTGGTTCGTGTCGTTCTTCTTTNTTGTTTGTTGTTGTTTGTTAGTACGACAACGAAGAAA
TARGET=172,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00193 Ps_contig_mira-and-tgicl-ass_franssen_2422
SEQUENCE=GATGTTTTTCGTTGAATCTTCACAGAGAAAGTAAATTAGAGAGAGAGAGTAGAAAAGCTTGAAGCTGTATAAGAGACATCAGTGTCTCTAGTAGAGKGTGTTTGTCTTGTGAGAGAGAGAAAGTAAAGTGAATAGTTGGTGAAGATGATGAATAACTCAGTWAGTTTACAAGACAACTAGTTTTATTATTGAAAGTGAAGAAGAAGNTGATAAGGTATTTAACGATAGGAATTGTTTCTGTATTTCCTTTACTTAAAAA
TARGET=38,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00194 Ps_contig_mira-and-tgicl-ass_franssen_2422
SEQUENCE=GATGTTTTTCGTTGAATCTTCACAGAGAAAGTAAATTAGAGAGAGAGAGTAGAAAAGCTTGAAGCTGTATAAGAGACATCAGTGTCTCTAGTAGAGKGTGTTTGTCTTGTGAGAGAGAGAAAGTAAAGTGAATAGTTGGTGAAGATGATGAATAACTCAGTWAGTTTACAAGACAACTAGTTTTATTATTGAAAGTGAAGAAGAAGNTGATAAGGTATTTAACGATAGGAATTGTTTCTGTATTTCCTTTACTTAAAAA
TARGET=111,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00195 Ps_contig_mira-and-tgicl-ass_franssen_2428
SEQUENCE=CATCATCATCATCTTCTTCCTCCTCTTCATCTTCTTCATCCACTGAAACAGGTTTGCGAGGACCTTCCGCGTAAATCTCAGGATCGTGAACAATCAAAAGCTTGAAGCCCATTT
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00196 Ps_contig_mira-and-tgicl-ass_franssen_2452
SEQUENCE=TTTACCCGGTTGTCCTTTCCACCATACGGTATTGTACACCCGTACTTAATTTAACATAGAAAGAAGAGAAAAAAACAAACATTAGATTAGATCTAATCACTGTCGCTGCTTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCCATGGATCTTGTTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTCTCTCCCTCATGAATAGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTYGTTACTTCCTCTTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTAATTTKKCAAMGGWAAMCCCTTTCTTTTCCTCAGTTACCGGTTTGTTCCTTCAAACAACCTCAACTTTCNCAACTGGCACCGAAGTNTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTCNCACCTATTTTCTCCTTCCTCTTCACCTTCCCGCGTACTCTGCGTTNAA
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00197 Ps_contig_mira-and-tgicl-ass_franssen_2452
SEQUENCE=TTTACCCGGTTGTCCTTTCCACCATACGGTATTGTACACCCGTACTTAATTTAACATAGAAAGAAGAGAAAAAAACAAACATTAGATTAGATCTAATCACTGTCGCTGCTTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCCATGGATCTTGTTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTCTCTCCCTCATGAATAGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTYGTTACTTCCTCTTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTAATTTKKCAAMGGWAAMCCCTTTCTTTTCCTCAGTTACCGGTTTGTTCCTTCAAACAACCTCAACTTTCNCAACTGGCACCGAAGTNTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTCNCACCTATTTTCTCCTTCCTCTTCACCTTCCCGCGTACTCTGCGTTNAA
TARGET=163,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00198 Ps_contig_mira-and-tgicl-ass_franssen_2452
SEQUENCE=TTTACCCGGTTGTCCTTTCCACCATACGGTATTGTACACCCGTACTTAATTTAACATAGAAAGAAGAGAAAAAAACAAACATTAGATTAGATCTAATCACTGTCGCTGCTTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCCATGGATCTTGTTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTCTCTCCCTCATGAATAGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTYGTTACTTCCTCTTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTAATTTKKCAAMGGWAAMCCCTTTCTTTTCCTCAGTTACCGGTTTGTTCCTTCAAACAACCTCAACTTTCNCAACTGGCACCGAAGTNTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTCNCACCTATTTTCTCCTTCCTCTTCACCTTCCCGCGTACTCTGCGTTNAA
TARGET=514,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00199 Ps_contig_mira-and-tgicl-ass_franssen_2459
SEQUENCE=GTGGTATCAACGCAGAGTGTTATTTATTGTTTCTTTTACATTGTTATTCTTTTTGTTGTCCTTATTATTAGTATATAAATAAATAAACATGTACTTTTTCATCTGAGTTTTGGGATTCATGGAGGTCTTTGTTTTCTGAATAATAAATATTTAAAGTAACTAC
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3469
PRIMER_LEFT_PENALTY=2.159969
PRIMER_RIGHT_PENALTY=0.186949
PRIMER_LEFT_SEQUENCE=GTGGTATCAACGCAGAGTGTTA
PRIMER_RIGHT_SEQUENCE=CCATGAATCCCAAAACTCAGAT
PRIMER_LEFT=0,22
PRIMER_RIGHT=121,22
PRIMER_LEFT_TM=57.840
PRIMER_RIGHT_TM=60.187
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=122
PRIMER_PAIR_PENALTY_1=2.6861
PRIMER_LEFT_1_PENALTY=2.499147
PRIMER_RIGHT_1_PENALTY=0.186949
PRIMER_LEFT_1_SEQUENCE=GTGGTATCAACGCAGAGTGTTATT
PRIMER_RIGHT_1_SEQUENCE=CCATGAATCCCAAAACTCAGAT
PRIMER_LEFT_1=0,24
PRIMER_RIGHT_1=121,22
PRIMER_LEFT_1_TM=59.501
PRIMER_RIGHT_1_TM=60.187
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=2.7308
PRIMER_LEFT_2_PENALTY=2.159969
PRIMER_RIGHT_2_PENALTY=0.570821
PRIMER_LEFT_2_SEQUENCE=GTGGTATCAACGCAGAGTGTTA
PRIMER_RIGHT_2_SEQUENCE=CATGAATCCCAAAACTCAGATG
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=120,22
PRIMER_LEFT_2_TM=57.840
PRIMER_RIGHT_2_TM=59.429
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=121
PRIMER_PAIR_PENALTY_3=2.8301
PRIMER_LEFT_3_PENALTY=2.159969
PRIMER_RIGHT_3_PENALTY=0.670093
PRIMER_LEFT_3_SEQUENCE=GTGGTATCAACGCAGAGTGTTA
PRIMER_RIGHT_3_SEQUENCE=AAACAAAGACCTCCATGAATCC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=133,22
PRIMER_LEFT_3_TM=57.840
PRIMER_RIGHT_3_TM=59.330
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=134
PRIMER_PAIR_PENALTY_4=2.8548
PRIMER_LEFT_4_PENALTY=2.667901
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=TGGTATCAACGCAGAGTGTTAT
PRIMER_RIGHT_4_SEQUENCE=CCATGAATCCCAAAACTCAGAT
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=121,22
PRIMER_LEFT_4_TM=57.332
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_00200 Ps_contig_mira-and-tgicl-ass_franssen_2461
SEQUENCE=TTTGTTGGCTTACCCAAAACGGGAAACGTAGAAAGACTTGGGTTTAGGGGAAAACGTTAAAGTAGGGAAGGGCGTAAAATTTACTTAGGGAAAGAACAGAACAAGCCCTCTACCTGTGGAAATAAATACAAACAAACCAAAAGGACTTTTTTAGGTTCTTACAAAAACCTAACAATTCTATTGGATTCTTGGAGCTGGCCATTTTGTACCTACTGATCAACCTTGTGTTGCATTAGAAATGATAGCTTCAATTACACAGTCACCAGCTGCTTGAAAAAGAAAACATTAGTACTATCTGTAGAATAAATTAAAGAAAAAAAGTCCAGGAAACAAAATTCATTGTGGATCAGGGAAAGAACAGCAATAAAGTCATGGAAAAGTGTTATTTATTGTTTCTTTTACATTGTTATTCTTTTTTGTTGTCCTTATTATTAGTATATAAATAAATAAACATGTACTTTTTCATCTGAGTTTTGGGATTCATGGGAGGTTCTTTTGTTTTCTGAAATAATAAAAATATTTAAAGTAACAAAAACG
TARGET=439,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2243
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.186949
PRIMER_LEFT_SEQUENCE=CTGATCAACCTTGTGTTGCATT
PRIMER_RIGHT_SEQUENCE=CCATGAATCCCAAAACTCAGAT
PRIMER_LEFT=212,22
PRIMER_RIGHT=485,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.187
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.4276
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.390220
PRIMER_LEFT_1_SEQUENCE=CTGATCAACCTTGTGTTGCATT
PRIMER_RIGHT_1_SEQUENCE=TCAGAAAACAAAAGAACCTCCC
PRIMER_LEFT_1=212,22
PRIMER_RIGHT_1=505,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.610
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=0.5232
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.485895
PRIMER_LEFT_2_SEQUENCE=CTGATCAACCTTGTGTTGCATT
PRIMER_RIGHT_2_SEQUENCE=CAGAAAACAAAAGAACCTCCCA
PRIMER_LEFT_2=212,22
PRIMER_RIGHT_2=504,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=60.486
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.5772
PRIMER_LEFT_3_PENALTY=0.186949
PRIMER_RIGHT_3_PENALTY=0.390220
PRIMER_LEFT_3_SEQUENCE=ATTCATTGTGGATCAGGGAAAG
PRIMER_RIGHT_3_SEQUENCE=TCAGAAAACAAAAGAACCTCCC
PRIMER_LEFT_3=334,22
PRIMER_RIGHT_3=505,22
PRIMER_LEFT_3_TM=60.187
PRIMER_RIGHT_3_TM=59.610
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.5815
PRIMER_LEFT_4_PENALTY=0.394568
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=CCAGCTGCTTGAAAAAGAAAAC
PRIMER_RIGHT_4_SEQUENCE=CCATGAATCCCAAAACTCAGAT
PRIMER_LEFT_4=262,22
PRIMER_RIGHT_4=485,22
PRIMER_LEFT_4_TM=60.395
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_00201 Ps_contig_mira-and-tgicl-ass_franssen_2467
SEQUENCE=GKMRKRKTCCGAGTATATAGATAACCAGAGGCAGATTTTAGTGATKGGAGATATTAGGGTTTAGAGAAACCACCAGCGGAGAAGCAGCCACCAGCGGAGAAGCAACAATGGTTCTCAAGACTGAACTCTGCCGATTCAGTGGTGCAAAGATCTACCCAGGAAGAGGCATCAGATTTATTCGCAGCGATTCTCAGGTGTTCCTATTTGTGAACTCAAAATGTAAGAGGTATTTCCACAACCGTTTGAAGCCTTCAAAGCTCACATGGACTGCCATGTATAGAAAGCAACACAAGAAGGACATTGCTCAAGAAGCTGTAAAGAAGAGACGTCGTGCTACCAAGAAGCCATACTCTAGGTCAATTGTTGGTGCTACCCTGGAAGTCATTCAGAAGAAAAGAGCCGAGAAGCCTGAAGTTCGAGATGCAGCTAGGGAGGCTGCTCTGCGTGAAATCAAAGAGAGGATTAAGAAAACAAAGGATGAGAAGAAAGCCAAGAAAGCAGAAGTAGCATCTAAGGCACAAAAGTCAGGCAAAGGCAATGTTCAGAAAGGAGGTTTGCCCAAGGGTCCTAAATTGGGCGGCGGCGGCGGTAAACGTTAAGTAGTTGCAATTTTGGCTGTAATTTGAATACACTTGTTTTTAAACTTCTATATTTATATTTTATTTGCTCGATTAGCTGACCTTATTATCACTCGTGTTTGGAGCTTCAGACACTACCTTATTGCTGAATGCATTTTACTAGCAATTCTATATTTTGTCTGCTAATAATTTGCATTGTTTAAATGGTTGTCTGTCTACGAGTCTCATAGCTCAAACATGCTTTTTATTTCTAATGTTGC
TARGET=576,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00202 Ps_contig_mira-and-tgicl-ass_franssen_2472
SEQUENCE=TTACCCCTAAAAACCCCTTACGGGTAACCGTTTTAAAAAAACGTTAAAAATTTTTTAAAAAACGGTTTTAAGGGTTACCCTAAGGGGTAACCCCCGAACCGAAAACCCCTTAAAAAACCCAAACCTAATTCCCTAAACGACTCCAATCATGATATAGATCGAAGCATATGCAAATTTATTAAGCCAAAACAGAGCACCTTCTCTCCCACATAAAGCTCCATTGCCTTCTCTTGAAAACTCATTTCCTCCCATTTCTTAGGAGGAGCATCTGCCTTACTGGGTTGCTCTATGCTATTTGTAACAGTTCCTTTCTCCCTCTGCCGTTTCGCTTGTCTCCTTGTTCTTCTCCTGAGCCTTAGCTCAACCAGTTTAGCCGGTGTTGTAGTTGGTGCTTGAATTGAGTTTGTTTCATCCTGGTCTGTTTCGGCAGCGTTGCTGTTGCTGCTGCTGCTGCATGTGATGGAATAGAAAAGGGAGAGAAGT
TARGET=440,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8841
PRIMER_LEFT_PENALTY=0.071684
PRIMER_RIGHT_PENALTY=1.812450
PRIMER_LEFT_SEQUENCE=GTTTCGCTTGTCTCCTTGTTCT
PRIMER_RIGHT_SEQUENCE=CTCCCTTTTCTATTCCATCACA
PRIMER_LEFT=322,22
PRIMER_RIGHT=476,22
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=58.188
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=1.9350
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=1.812450
PRIMER_LEFT_1_SEQUENCE=TTAGGAGGAGCATCTGCCTTAC
PRIMER_RIGHT_1_SEQUENCE=CTCCCTTTTCTATTCCATCACA
PRIMER_LEFT_1=255,22
PRIMER_RIGHT_1=476,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=58.188
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=1.9428
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=1.812450
PRIMER_LEFT_2_SEQUENCE=AGCACCTTCTCTCCCACATAAA
PRIMER_RIGHT_2_SEQUENCE=CTCCCTTTTCTATTCCATCACA
PRIMER_LEFT_2=192,22
PRIMER_RIGHT_2=476,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=58.188
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=1.9428
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=1.812450
PRIMER_LEFT_3_SEQUENCE=GCACCTTCTCTCCCACATAAAG
PRIMER_RIGHT_3_SEQUENCE=CTCCCTTTTCTATTCCATCACA
PRIMER_LEFT_3=193,22
PRIMER_RIGHT_3=476,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=58.188
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=1.9428
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=1.812450
PRIMER_LEFT_4_SEQUENCE=CACCTTCTCTCCCACATAAAGC
PRIMER_RIGHT_4_SEQUENCE=CTCCCTTTTCTATTCCATCACA
PRIMER_LEFT_4=194,22
PRIMER_RIGHT_4=476,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=58.188
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_00203 Ps_contig_mira-and-tgicl-ass_franssen_2483
SEQUENCE=TACGTCGGCGGATGGGGGGATCTGTGTCAATCCCAACATAGTATTCATCTATGGCAGCAACAATGGCAATGATGATCAACTCCAAGTGTCTAAACACAGCCATACCCAAGTTACAAAACACNTCTACCACAGCGAAACTAACAACAACAACATGCAAAAACCCACCACCGTTACAAAACCTAACCAAGGCTATTACCTCATTTGACAACACCACGTTGTTGTGTCACCTTCCATTGCAATT
TARGET=140,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3928
PRIMER_LEFT_PENALTY=0.080323
PRIMER_RIGHT_PENALTY=0.312496
PRIMER_LEFT_SEQUENCE=CACAGCCATACCCAAGTTACAA
PRIMER_RIGHT_SEQUENCE=CAATGGAAGGTGACACAACAAC
PRIMER_LEFT=94,22
PRIMER_RIGHT=235,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.312
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=142
PRIMER_PAIR_PENALTY_1=0.4066
PRIMER_LEFT_1_PENALTY=0.339170
PRIMER_RIGHT_1_PENALTY=0.067419
PRIMER_LEFT_1_SEQUENCE=CAATGGCAATGATGATCAACTC
PRIMER_RIGHT_1_SEQUENCE=GCCTTGGTTAGGTTTTGTAACG
PRIMER_LEFT_1=60,22
PRIMER_RIGHT_1=189,22
PRIMER_LEFT_1_TM=60.339
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=0.4157
PRIMER_LEFT_2_PENALTY=0.103216
PRIMER_RIGHT_2_PENALTY=0.312496
PRIMER_LEFT_2_SEQUENCE=AACACNTCTACCACAGCGAAAC
PRIMER_RIGHT_2_SEQUENCE=CAATGGAAGGTGACACAACAAC
PRIMER_LEFT_2=116,22
PRIMER_RIGHT_2=235,22
PRIMER_LEFT_2_TM=59.897
PRIMER_RIGHT_2_TM=60.312
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=0.5368
PRIMER_LEFT_3_PENALTY=0.339170
PRIMER_RIGHT_3_PENALTY=0.197640
PRIMER_LEFT_3_SEQUENCE=CAATGGCAATGATGATCAACTC
PRIMER_RIGHT_3_SEQUENCE=CCTTGGTTAGGTTTTGTAACGG
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=188,22
PRIMER_LEFT_3_TM=60.339
PRIMER_RIGHT_3_TM=59.802
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.6174
PRIMER_LEFT_4_PENALTY=0.419726
PRIMER_RIGHT_4_PENALTY=0.197640
PRIMER_LEFT_4_SEQUENCE=TGTCTAAACACAGCCATACCCA
PRIMER_RIGHT_4_SEQUENCE=CCTTGGTTAGGTTTTGTAACGG
PRIMER_LEFT_4=86,22
PRIMER_RIGHT_4=188,22
PRIMER_LEFT_4_TM=60.420
PRIMER_RIGHT_4_TM=59.802
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_00204 Ps_contig_mira-and-tgicl-ass_franssen_2485
SEQUENCE=GTTCGTTCGGGTTTATWGATCACTTGCAATCTTCTCTTTCTCATTTTCAATCATTCTTCCAGAAACCCTTTTCTTTCGTTTCAATCTTTTCATCTGCCATGGCGCCCAAGGGAGAGAAGAAACCAGCGGCAAAGAAGCCTGCAGAGAAGGCACCAGTAGAACCCAAGGCTGAGAAAAAGATCCCGAAGGATGCTTCCTCCACCGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAGACGTACAAGATCTACATCTTCAAGGTTCTTAAGCAAGTTCATCCTGACATCGGAATCTCCAGCAAAGCTATGGGGATCATGAATAGCTTCATCAATGATATCTTCGAGAAGCTCGCTCAGGAGTCTTCTAGACTCTCTCGCTACAACAAAAAATCCACCATCTCTTCCCGGGAGATCCAGACTGCTGTTCGTCTTGTTCTTCCCGGTGAGCTTGCCAAGCACGCCGTTTCCGAGGGAACCAAATCTGTCACCAAGTTCACCAGTTCTGATTAGAAATACTGATTTAAATTAGGGTTTACTTGATGTATATTTTGGATTTTCTCGTTTTCGTTTTAGTAAAGAAATATCTCTAATGAAATTAATTTTTGGGAC
TARGET=207,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00205 Ps_contig_mira-and-tgicl-ass_franssen_2487
SEQUENCE=GTCGGGGGATCGCTTTCTCATTCTCGATTTTTCTTCCAGAAACCCTTTTCTTTCGATCAAATCTTTTCATCATCCATGGCCCCTAAGGGAGAGAAAAAACCAGCGGAGAAGAAGCCCGTAGAGAAACCTAAGGCTGAGAAAAAGATCCCGAAGGACGTTTCCTCCACCGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAGACGTACAAGATCTACATCTTCAAGGTTCTTAAGCAAGTTCATCCTGACATTGGAATCTCCAGCAAAGCTAATGGGGATCATGAAAACAGCTTCATCAATGATATCTTCGAGAAGCTCGCTCAGGAGT
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3171
PRIMER_LEFT_PENALTY=0.093130
PRIMER_RIGHT_PENALTY=0.224014
PRIMER_LEFT_SEQUENCE=CCGTAGAGAAACCTAAGGCTGA
PRIMER_RIGHT_SEQUENCE=CCATTAGCTTTGCTGGAGATTC
PRIMER_LEFT=115,22
PRIMER_RIGHT=277,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.224
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.3980
PRIMER_LEFT_1_PENALTY=0.174021
PRIMER_RIGHT_1_PENALTY=0.224014
PRIMER_LEFT_1_SEQUENCE=GATCGCTTTCTCATTCTCGATT
PRIMER_RIGHT_1_SEQUENCE=CCATTAGCTTTGCTGGAGATTC
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=59.826
PRIMER_RIGHT_1_TM=60.224
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=0.4206
PRIMER_LEFT_2_PENALTY=0.093130
PRIMER_RIGHT_2_PENALTY=0.327468
PRIMER_LEFT_2_SEQUENCE=CCGTAGAGAAACCTAAGGCTGA
PRIMER_RIGHT_2_SEQUENCE=ATGAAGCTGTTTTCATGATCCC
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=298,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=60.327
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=0.4207
PRIMER_LEFT_3_PENALTY=0.093130
PRIMER_RIGHT_3_PENALTY=0.327533
PRIMER_LEFT_3_SEQUENCE=CCGTAGAGAAACCTAAGGCTGA
PRIMER_RIGHT_3_SEQUENCE=CTGGAGATTCCAATGTCAGGAT
PRIMER_LEFT_3=115,22
PRIMER_RIGHT_3=265,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=60.328
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.4784
PRIMER_LEFT_4_PENALTY=0.254402
PRIMER_RIGHT_4_PENALTY=0.224014
PRIMER_LEFT_4_SEQUENCE=AGAAGAAGCCCGTAGAGAAACC
PRIMER_RIGHT_4_SEQUENCE=CCATTAGCTTTGCTGGAGATTC
PRIMER_LEFT_4=106,22
PRIMER_RIGHT_4=277,22
PRIMER_LEFT_4_TM=60.254
PRIMER_RIGHT_4_TM=60.224
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_00206 Ps_contig_mira-and-tgicl-ass_franssen_2506
SEQUENCE=TGGTTTGAAACGTTTCCGGTCAACTCCGGCGAGCTGGATAGAATCGTTTATTCTCAAAGAAGAAGAAGAGGAGGACGAAAACCAACAACAAGACAATTTCAGTTTCACTCAACTGCTTTCCAATAACGCTGCTGCCGGTCCTTCAACTCCCGATTCTCATCCATATTTACCTGATTATTACCAGTACTCTTCACCGCATACTCTTTCTGCCGCAGATGCCTCTAATAATCCCTTCACACAGCCAAAAAGTATTCAAATATTCAAGACAAAATGAGACAGAAGGAGGATTGACTATACGAAAAGATAATCAAATAGTTACAGAAAGATGATATGAATTAAATTGTGTATATACTGATTTGGAGTATTATAACATAGACATTATGAGGTTGTTTGATTTGTATAAACAAACCACTGCTTAGGGGTTGAGGAGAGTGATAATGCATTGGATCTTAAAATAGACAAAATTTTAGAGGATTCGGTTCCTTGCAAGATTCGGGCAAAGCGTGGCTGTGCTACACATCCAAGGAGTATTGCTGAAAGGGTAACTGTTAATACTAATTTGGGATTTAGACTTAATCCGAATTGTGTGAATCTTTTCTGTTTT
TARGET=57,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1934
PRIMER_LEFT_PENALTY=0.710129
PRIMER_RIGHT_PENALTY=0.483253
PRIMER_LEFT_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_SEQUENCE=CAGAAAGAGTATGCGGTGAAGA
PRIMER_LEFT=28,22
PRIMER_RIGHT=208,22
PRIMER_LEFT_TM=60.710
PRIMER_RIGHT_TM=59.517
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=1.2362
PRIMER_LEFT_1_PENALTY=0.710129
PRIMER_RIGHT_1_PENALTY=0.526059
PRIMER_LEFT_1_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_1_SEQUENCE=TGGCTGTGTGAAGGGATTATTA
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=243,22
PRIMER_LEFT_1_TM=60.710
PRIMER_RIGHT_1_TM=59.474
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=5.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=216
PRIMER_PAIR_PENALTY_2=1.4178
PRIMER_LEFT_2_PENALTY=0.710129
PRIMER_RIGHT_2_PENALTY=0.707667
PRIMER_LEFT_2_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_2_SEQUENCE=AAATATGGATGAGAATCGGGAG
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=167,22
PRIMER_LEFT_2_TM=60.710
PRIMER_RIGHT_2_TM=59.292
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=1.4316
PRIMER_LEFT_3_PENALTY=0.710129
PRIMER_RIGHT_3_PENALTY=0.721451
PRIMER_LEFT_3_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_3_SEQUENCE=TTGGCTGTGTGAAGGGATTATT
PRIMER_LEFT_3=28,22
PRIMER_RIGHT_3=244,22
PRIMER_LEFT_3_TM=60.710
PRIMER_RIGHT_3_TM=60.721
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=1.4316
PRIMER_LEFT_4_PENALTY=0.710129
PRIMER_RIGHT_4_PENALTY=0.721451
PRIMER_LEFT_4_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_4_SEQUENCE=TTTGGCTGTGTGAAGGGATTAT
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=245,22
PRIMER_LEFT_4_TM=60.710
PRIMER_RIGHT_4_TM=60.721
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=5.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_00207 Ps_contig_mira-and-tgicl-ass_franssen_2511
SEQUENCE=AGCAAATATCATTCCTACATGAGCAACAATTCGTCTCTCTCTCTAACCCAGAGCAACAATATGCTCTGCAACTTCCGACCCAACCCACAGCCAAATCCCACTGGAGAAGACGGTGGCGGCCGGCCAACGGCGGCGGCGCCACCTTCTTCTCCGGCGGACCTCTACCTCACAACTTAAATTCCAAACACTAACCCTACCTAGTCTAACCTCACGATCACAAATCCGGTCACGTAAACACCAAAAGGTTAACCTAAAGCCTTGGATCGGAAAACCCTAACCATACACAAAAAATCTAAACAAAGTTCTTTACAAACCAAACCATATAAATCTTCTCCTCAAGCCAAAACAAACACAAAAGCAAATGAACACTAACATTCAGAGTCAAAACCCTAAGTCTACAAATGAAGCCACAACGGCGGAGATTTTAAGCAATTGACATGGGGGTTTTGGTCGGCTTATCAAGACGAATGAGTTGGTGCAAATATTTAAAGCGAAAGAGAAAGAAGAAGTACTTGACCGGAAGTGGGTCGCCGACAACGTCTTCTCCGCGGCCGTTTTCAGATAAGCTCCTTTCTAATAAACCAACTCCTTTCGTCTTACTCTGTTCTTTCTTAACTTTGAGCTCCTTTAGATGGTTGTGTTGATGAAATATTATTTTGATTTCTTTTTCGTTTCTTGATTCACTCCCCTTTTCAATGTCCTCTGTTCTGGGTATTTATGTTCTTTTCATGATGAATTTATTCAGTCTTGAGATAACTTGTTAGGTTTCTTTTTTCTTATCCCCAGATTTCATGGGATTGTTGTTTTGTGGTTTCATTGTTGTTTGAACTGGTTAACGGAACGCATTCTTGGGTGTTCATGGTAGTCTTTTAGGCAAAACATTAACCTAAGCGTTTTGTTAATTATCCTTCTAATTTTTTAAGCGCTTATTAGAAAAAGTACTTTTAACTTAGATTGTCAACTTCCTTCGNAGGTCAACAAAATCAATTGAAGGAAAAAAGGAACGNTNTNTATTGTTCTAAGAAAGGAGGGTTTGTAGTAA
TARGET=34,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2398
PRIMER_LEFT_PENALTY=0.130883
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=TTCCTACATGAGCAACAATTCG
PRIMER_RIGHT_SEQUENCE=GTGTATGGTTAGGGTTTTCCGA
PRIMER_LEFT=11,22
PRIMER_RIGHT=284,22
PRIMER_LEFT_TM=60.131
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.6000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.5218
PRIMER_LEFT_1_PENALTY=0.130883
PRIMER_RIGHT_1_PENALTY=0.390906
PRIMER_LEFT_1_SEQUENCE=TTCCTACATGAGCAACAATTCG
PRIMER_RIGHT_1_SEQUENCE=TGTATGGTTAGGGTTTTCCGAT
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=283,22
PRIMER_LEFT_1_TM=60.131
PRIMER_RIGHT_1_TM=59.609
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=1.1054
PRIMER_LEFT_2_PENALTY=0.130883
PRIMER_RIGHT_2_PENALTY=0.974470
PRIMER_LEFT_2_SEQUENCE=TTCCTACATGAGCAACAATTCG
PRIMER_RIGHT_2_SEQUENCE=TGTGTATGGTTAGGGTTTTCCG
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=285,22
PRIMER_LEFT_2_TM=60.131
PRIMER_RIGHT_2_TM=60.974
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=1.3090
PRIMER_LEFT_3_PENALTY=0.130883
PRIMER_RIGHT_3_PENALTY=1.178157
PRIMER_LEFT_3_SEQUENCE=TTCCTACATGAGCAACAATTCG
PRIMER_RIGHT_3_SEQUENCE=GTTAGGGTTTTCCGATCCAAG
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=277,21
PRIMER_LEFT_3_TM=60.131
PRIMER_RIGHT_3_TM=59.822
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=1.3424
PRIMER_LEFT_4_PENALTY=0.130883
PRIMER_RIGHT_4_PENALTY=1.211563
PRIMER_LEFT_4_SEQUENCE=TTCCTACATGAGCAACAATTCG
PRIMER_RIGHT_4_SEQUENCE=TATGGTTAGGGTTTTCCGATCC
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=281,22
PRIMER_LEFT_4_TM=60.131
PRIMER_RIGHT_4_TM=61.212
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.6000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_00208 Ps_contig_mira-and-tgicl-ass_franssen_2512
SEQUENCE=GTGAGMAAAKATCATTCCTATATGAGCAACAATTCGTCTCTCTCTCTAACCCAGAGCAACAATATGCTCTGCAACTTCCGACCCAACCCACGGCCAAATCCCACTGGAGAAGACGGTGGCGGCCGGTCAACGGCGGCGGCGCCGCCTTCTTCTCCGGTGAACCTCTACCTCACAACTTAAATTCCAAACACTAACCCTACCTATTCTAGCCTCACGATCACAAATCCGGTCACGAAAACACCAAAAGGTTAACCTAAAGCCTTGGATCGGAAAACCCTAACCATACACAAAAATCTAAACAAAGTTCTTTACAAACCAAATCATATAAATCTTCTCCTCAAGCCAAAACAAACACAAAAGCAAATGAACACTAACATTCAGAGTCAAAACCCTAAGTCTATAAATGAAGCCACAACGGCGGAGATTTTAAGCAATTGACATGGGGTTTTTGGTCGGCTTATCAAGACGAACGAGGTGGTGCAAATATTTAAAGCGAAAGAGAAAGAAGAAGTACTTGACCGGAAGTGGGTCGCCGACAACGTCTTCTCCGCGGCCGTTTTCAGATAAGCTCCTTTCTAATAATCCAACTCCCTTCGTCTTACTCTGTTATTTTTTAACTTTGAGCTCCTTTAGATGGTTGTGTTGATGAAAGATTATTTTGATTTCTCTTTCGTTTCTTCCTTCACTCTCCTTTTCAATGTCCTCTGTTCTGGGTATTTATGTTCTTTTCATGATGAATTTATTCAGTCTTGAGATAACTTGTTAGGTTTCTTTTTTTCTTATCCCCAGATTTCATGGGATTGTTGTTTTGTGGTTTCATTGTTGTTTGAACTGGTTAACGGAATGCATTCTTGGGTGTTCGGGGTAGTCTTTTAGGCAAAACATTAACCTGAGCGTTTTGTTAATTATCCTTCAGTTTTTAAGCGCTTATTGAAAAAGCTTTTAACTTAGATTGTCACTTCCTTCGCAGGTCACAAAATCAATTGAGGAAAAAAGGAGCGGATGTATGTTGGTTCTAAGAAGAGGTTGGGATTTTTAGAGTGCTTTGGCCCAAGAGATGACGTTGGCCGCTGGCTTTTTGGAAATTCCATCTTCACACTGCTGGACAATGCAGCAACCTTTAATTTTCAAAATTAGTCATTTAAGATGTACTTTGGACTTTTGGAACGTATTGGATAATGTATCAGCCTTTGGATAATTATGTAACTTTGGGAATTTTGTAACATTTTGGATTAGAATTCATTTCCGTAATTAATCCTTTTTAATCATTCHAAACTTGACYTAG
TARGET=37,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00209 Ps_contig_mira-and-tgicl-ass_franssen_2514
SEQUENCE=AAAAACAACAAAATAAACTACTTGGCTCAAAGCTTTACCCTTAACAGATTAACATGAATACTCAAAAAARCRCTTCGAAAATAACTTAAAACAGTAACACATTTACAAAAATGGAATCCACAATAACCGGGAATGGAAAGGCATAACAGACGGTTTTTCTTTTGAGGATAAAAAATTCATCTGTGAAGAGATCAGGAGATATATAAGAACACGTCTAAAAAATCCAGGGGGACGGAGATACTTCTTTGTAAGCATAAAATCCAAAAAACAACACAACATACGTTTTGGGGAAATCTAAGAGAAGCAAWTAAAATTGTTTTTCTCTGTTAAACTCAAAAAATATTTCCTTAACACGAAGCCAGATTCAACAGTGAATCAAGAAACAAAAAATAATCTTTCATCAACACAACCATCTAAAGGAGCTCAAAGTTAAACAAGAACAGAGTAAGACGAAGGGAGTTGGATTATTAGAAAGGAGCTTATTTGGAAACAACCGCGGAGAAGACGTCGCCGGCGACCCACTTCCGGTCAAGTAATTCTTCTTTCTCCTTCGCTTTAAATATTTGCACCACCTCGTTCGTCTTGATAAGCCGACCAAAACCCTCATGTCAATTGCTTAAAATCTCCGCTGTTGTGGCTTCATTTGTAGACTTAGGGTTTTGACTCTGAATGTTGGTGTTTATTTGCTTTTGTGTTTGTTTTGGCTTGAGGAGAAGATTTATGTGGTTTGGTTTGTAAAGAACTTTGTTTAGATCTTTGTGTGTGGTTGGGGTTTTCCGATCCAAGGCTTTAGGTTAACCTTTTGGTGTTTTCGTGACCGGATTTGTGATCGTGAGGTTAGAATAGGTAGGGTTAGTGTTTGGAATTTAAGCTGTGAGGTAGAGGTTTGTCGGAGAAGAAGGCGGCGCCGCCGCCGTTGGCTGGCCGCCACCGTCTTCTCCGGTGGGATTTGGTCGTGGGTTGGGTCGGAAGTTGCAGAGCATATTGTTGCTCTGGGTTAGAGAGAGAGAGACGAATTGTTGCTCATGTAGGAATGATATTTGCTCCCCCGAGTACT
TARGET=1000,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00210 Ps_contig_mira-and-tgicl-ass_franssen_2516
SEQUENCE=GATTCCTACATGAGTAACAAATCGTCTCTCTCTCTAACTCAGAGCAACAATGTGCTCTGCAACTTCCGACCCAACCCACAGCCAAATCCCACCGGAGAAAACGGTGGCGGCCGGTCAACGGTGGCGGCCGGTCAACGGTGGCGGCGGCGCCTTCTTCTTCGGCGAACCTCTACCTCAGAGCTTAAATTCCAAACACTAACCCTACCTATTCTAACCTCGCGATCACAGATCCGGTCACGAAAACAA
TARGET=25,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00211 Ps_contig_mira-and-tgicl-ass_franssen_2524
SEQUENCE=CCAAGAAGTGTGAGTTCAATTGGGGATTGTTTGTTATTTAGTTTGAAGGTTGGGAAGGTGAAGCTTATTGGGTTCATGTTTGGACATTGAAAAAATGGGTTTGATTACGCAATTTAGAGAGTATTTTAATACATGGCTTGTTGTGAGAGATTTGAGGCCATTGAGATGGGAAGAAGATCATCATCATCAATAAGCATGATAGTATGATGTACTAGTTTGGTAGGGAAGTAAACGGTTACCCTAAACCCGTTCGGTTAGTTAACTTTTCCGTTTTTAACGGTTCCGGGTTTAAACGGGGTTTACCCCGTTTAAACCCCGGGTTTTTTACCCCCGGGGGGGTTTAAACCGTTTTTTGGGGGTTTTACGGGGTTTTTTAACCGGGGGTTTAAACCCTTTTTTAAAAAACGGTTTTTTTAAAAAACCCGGGAAAAAAACCCCTTAAAACCGGTTAAAAAGGTTTAAAACCGGGTAAAAAACCCGTTTTAAAAAGGGTTTTTTTAGGGGGTTTTTTTTAACGGGGTTTTAACCGGGTTTTTTACCCGGTTTTTTAAGGGGTTTTTACGGTTTTTTTTTAAACCCGG
TARGET=177,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5520
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.551271
PRIMER_LEFT_SEQUENCE=GTTGGGAAGGTGAAGCTTATTG
PRIMER_RIGHT_SEQUENCE=TAACTAACCGAACGGGTTTAGG
PRIMER_LEFT=48,22
PRIMER_RIGHT=260,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.449
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.6468
PRIMER_LEFT_1_PENALTY=0.095574
PRIMER_RIGHT_1_PENALTY=0.551271
PRIMER_LEFT_1_SEQUENCE=GTGAGTTCAATTGGGGATTGTT
PRIMER_RIGHT_1_SEQUENCE=TAACTAACCGAACGGGTTTAGG
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=260,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.449
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.7880
PRIMER_LEFT_2_PENALTY=0.236697
PRIMER_RIGHT_2_PENALTY=0.551271
PRIMER_LEFT_2_SEQUENCE=AGCTTATTGGGTTCATGTTTGG
PRIMER_RIGHT_2_SEQUENCE=TAACTAACCGAACGGGTTTAGG
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=260,22
PRIMER_LEFT_2_TM=60.237
PRIMER_RIGHT_2_TM=59.449
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=0.8163
PRIMER_LEFT_3_PENALTY=0.265053
PRIMER_RIGHT_3_PENALTY=0.551271
PRIMER_LEFT_3_SEQUENCE=AGTGTGAGTTCAATTGGGGATT
PRIMER_RIGHT_3_SEQUENCE=TAACTAACCGAACGGGTTTAGG
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=260,22
PRIMER_LEFT_3_TM=59.735
PRIMER_RIGHT_3_TM=59.449
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.8163
PRIMER_LEFT_4_PENALTY=0.265053
PRIMER_RIGHT_4_PENALTY=0.551271
PRIMER_LEFT_4_SEQUENCE=AAGTGTGAGTTCAATTGGGGAT
PRIMER_RIGHT_4_SEQUENCE=TAACTAACCGAACGGGTTTAGG
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=260,22
PRIMER_LEFT_4_TM=59.735
PRIMER_RIGHT_4_TM=59.449
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.3000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_00212 Ps_contig_mira-and-tgicl-ass_franssen_2525
SEQUENCE=CCGTTTTAACCTTTTAATTTTTACCTAACTTTACTTTTTTTTCCCTTTTCCCTAAAATTTAGGTTGATAACTTTACGCATAACCAATTAACCACAAGACATGAAAAGGATGTACACAACACATTACATTAAAACAACCAAATAAAACACAAGACACAAACATTGTCATGTGTTCTAAATAGCCAACACAAGTCCCGGAAGTGACCTACGATAAAGATCACGAGGTTGACTTCTCCACAATGTCATCATACTATCATGCTTATGATGATGATGATCTTCTTCCCATCTCAATGGCCTCAAATCTCTCACAACAAGCCATGTATTAAAATACTCTCTAAATTGCGTAATCAACCCATTTTTCAATGTCCAAACATGAACCCAATAAGCTTCACCTTCCCAACCTTCAACTATAACACAATCCCCAATTGAACTCACACTTCTTGGCTCAAATTTGAACCCTTTTTTGTGATCTATCTCACCCGTTAACACCTTCATCATGTGTTGACATTTTGGTGGACCATGGAACCAATACTCGAGATCACTTGCTAGCAATTTTGATACCGTTTGGAATTCCATTGCTCCTTGGCCTAGTAATGCTTTGTAGAGAGTCTCAACTATGGACTTGTTTTGTTTTTCAATCATTGAGTCCATTTTAGAGAGAATGGTTACTTTGTTGAGTTACCACTCCCCGACTACTCTGCGTTG
TARGET=261,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0155
PRIMER_LEFT_PENALTY=0.014720
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=CCGGAAGTGACCTACGATAAAG
PRIMER_RIGHT_SEQUENCE=GTTGGGAAGGTGAAGCTTATTG
PRIMER_LEFT=193,22
PRIMER_RIGHT=399,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.1103
PRIMER_LEFT_1_PENALTY=0.014720
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=CCGGAAGTGACCTACGATAAAG
PRIMER_RIGHT_1_SEQUENCE=GAGTTCAATTGGGGATTGTGTT
PRIMER_LEFT_1=193,22
PRIMER_RIGHT_1=431,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.2299
PRIMER_LEFT_2_PENALTY=0.014720
PRIMER_RIGHT_2_PENALTY=0.215215
PRIMER_LEFT_2_SEQUENCE=CCGGAAGTGACCTACGATAAAG
PRIMER_RIGHT_2_SEQUENCE=CAAATTTGAGCCAAGAAGTGTG
PRIMER_LEFT_2=193,22
PRIMER_RIGHT_2=452,22
PRIMER_LEFT_2_TM=60.015
PRIMER_RIGHT_2_TM=59.785
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.2514
PRIMER_LEFT_3_PENALTY=0.014720
PRIMER_RIGHT_3_PENALTY=0.236697
PRIMER_LEFT_3_SEQUENCE=CCGGAAGTGACCTACGATAAAG
PRIMER_RIGHT_3_SEQUENCE=AGCTTATTGGGTTCATGTTTGG
PRIMER_LEFT_3=193,22
PRIMER_RIGHT_3=386,22
PRIMER_LEFT_3_TM=60.015
PRIMER_RIGHT_3_TM=60.237
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=0.2555
PRIMER_LEFT_4_PENALTY=0.254773
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=AGATCACGAGGTTGACTTCTCC
PRIMER_RIGHT_4_SEQUENCE=GTTGGGAAGGTGAAGCTTATTG
PRIMER_LEFT_4=213,22
PRIMER_RIGHT_4=399,22
PRIMER_LEFT_4_TM=59.745
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00213 Ps_contig_mira-and-tgicl-ass_franssen_2527
SEQUENCE=GATCTATCCAACTTTACCACTTACTAATTTATTTATTCACAACATCATGAGTTKAGAAATTGGCAGCGGAATCATCGGAAGGTGRAGAAGAAGGAAAGYTAAGCGGCGGCAACCAGATATTGACTGTCGACGACGACCTCCGAGAATTGGGGAAAAAGGCCGCCTGGAGCGTCAGTTCCTGCAAACCCGGCAACRGCGTTTCTTCTCTTCGAGATGACAATCTCGAGACCTATTGGCAGTTACTCTCACTCACTCATCTTCTCATGCATTCTATTTTCATTAA
TARGET=245,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00214 Ps_contig_mira-and-tgicl-ass_franssen_2528
SEQUENCE=TAGTCTAACANACTTATCCTTACGTCTCACAAAACTACWATACCACAAAAGGACTTGTTTACTAATGTGCAAAAGACTTCAAAAATACTTAAACCATCAGAATATGCTGCTCTTCATGATTGTGCTGAAGAAATCAGTGATAGTGTTGATAGGCTTAGCCGTTCATTGAAAGAGCTTAAACTCTGCAAGATCAACGGTCAGGATTTCAATTGGCATATAAGCAATGTGGAGACATGGGTTAGCTCAGCTTTGACAGATGAGAGCACTTGTGGGGATGGATTTAGTGGAAAAGCACTTGATGGAAAAATCAAAGCTTCTATTAGATCTAGAATGGTTAATGTTGCTCAAGTTACTAGTAATGCTCTTTCACTTATTAATCAATATGCTGCTAAACACTAGGTAGAAATCAAACACTAGGAATCAAAGGTTTTGCTTTTAAGGTTGTTGAGAATGCCTTTAATGTTGTTGGTTCCTTTTTTATTTTGTTTTAATTTATGTGGTGTGTAATGTGTAATTACATCTATATCTATATATATAAAGAGATCACAAAACTTTGTCAAGTGTGAAATAAATTTTCTATTTATTTTTATTGTATTTTTTTTGTTTST
TARGET=528,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00215 Ps_contig_mira-and-tgicl-ass_franssen_2535
SEQUENCE=CCTAATTCACTCTTCTTCTTCTACCTTTCCAAACTTACTTGCATTAACATTCTCAAATTACAACACCAGAACTCCAATCCAATCCTCACCTTCTTCCACAAACATTTGCACCCAAAAAATACGACACATCTTTGATCACCATCGCCGAATCTTTCTACGATGATGAACTCTGGGCAGCTTCTTCCCTCCGTGTTCGTTCCTTCAATCAATTCCGTTCCCGGAACTTACGGAGTCCAATTTTCTACTTATGGAGAAGACAGGATAGTAGTTGGTTCTCTCGATCTTAATCAGTGCTTGAGTCTTCCGGATGAAATTGTAGGAATGAAGCCRGAGGTTAGCGGAGCTGATACGACAAGGGCTTACCTGAGTAATGTGTGTGTTGCTAGAGAGTTGCATAGGAATGGATTGGCCTATGAACTTCTTGAAAAGTCGAAGTTAGTTGCTCGTAATTGGGGCATAACAGATTTATACGTCCATGTTGCCGATGACAATGAACCAGACAAAAGAAATTGTACATAGGAAGTAGGTTGGGTTTTGTTTAACGGAAGG
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00216 Ps_contig_mira-and-tgicl-ass_franssen_2537
SEQUENCE=TTTWTTTACGGCTTAGAAAATAGAAAGGAAGGAAAGTAATGAGGCAATGAGGGAATGTTCTGAAAATAACTTAAAAAGAATAGACCATATTAACGCAGGATACCCGACAACACCGTCAAGTTAGGAGATTGAAAGACCCATCCATAGCAGAAGTCTTCGCGGTCGATCAAGAAACCCGGCCTGCCATGCAGGTTCATCACTCTCATAAACAAACCCGCTCTTCATGTACAATTTCTTTGCTGGTTCATTGTCATCGGCAACATGGACGTATAAATCTGTTATGCCCCAATTACGAGCAACTAACTTCGACTTTTCAAGAAGTTCATAGGCCAATCCATTCCTATGCAACTCTCTAGCAACACACACATTACTCAGGTAAGCCCTTGTCGTATCAGCTCCGCTAACTTGGACTCCGTAAGTTCCGGGACGGAATTGATTGAAGGAACGAACACGGAGGGAAGAAGCTGCCCAGAGTTCATCATCGTAGAAAGATTCGGCGATGGTGATCAAAGATGTGTCGATTTTTTGGGTGCAAATGTTTGTGGAAGAAGGTGAGGATTGGATTGGAGTTCTGGTGTTGTAATTTGAGAATGTTAATGCAGAATTTGGAAAGGTAGAAGAAGAAGAGTGAATTGAAGGGAAGGAACAGAGCGAAGAAATTAGCGTCATTTTTTGTTAGGTGTGTTGTTTGTTGTATTATTCGTCGTTGCATCCCCT
TARGET=616,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00217 Ps_contig_mira-and-tgicl-ass_franssen_2553
SEQUENCE=AGAGCTCCTTCGAATAAGCAGAATATTTTATTCATACCTTACACTGCAAATGATCTGGGATGCCACGGTGACTCCTCGTGGTGCGTGTAAGCCAACTGCACGTCGTCCCCAACCTGCAGCGACACCGCCGACGCCTCCTCTCACAACTTACTTACAAGTAAACGTAACGTCCGTCCGTCGTTCCGGTTACGGACGGTACGGTTCGGTTCCGGTACCGGTACCGGTCCGTCCGACCCAACCGACCGTCCGTTTCCCGGTTACCGGGAACGGGAACGGAACCGACGGACGGACGTACTACGTA
TARGET=168,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.6543
PRIMER_LEFT_PENALTY=0.115769
PRIMER_RIGHT_PENALTY=3.538580
PRIMER_LEFT_SEQUENCE=GAGCTCCTTCGAATAAGCAGAA
PRIMER_RIGHT_SEQUENCE=GAACCGTACCGTCCGTAACC
PRIMER_LEFT=1,22
PRIMER_RIGHT=203,20
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=61.539
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=60.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=3.7706
PRIMER_LEFT_1_PENALTY=0.232060
PRIMER_RIGHT_1_PENALTY=3.538580
PRIMER_LEFT_1_SEQUENCE=AGAGCTCCTTCGAATAAGCAGA
PRIMER_RIGHT_1_SEQUENCE=GAACCGTACCGTCCGTAACC
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=203,20
PRIMER_LEFT_1_TM=59.768
PRIMER_RIGHT_1_TM=61.539
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=3.9068
PRIMER_LEFT_2_PENALTY=0.368190
PRIMER_RIGHT_2_PENALTY=3.538580
PRIMER_LEFT_2_SEQUENCE=CTTACACTGCAAATGATCTGGG
PRIMER_RIGHT_2_SEQUENCE=GAACCGTACCGTCCGTAACC
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=203,20
PRIMER_LEFT_2_TM=59.632
PRIMER_RIGHT_2_TM=61.539
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=3.9068
PRIMER_LEFT_3_PENALTY=0.368190
PRIMER_RIGHT_3_PENALTY=3.538580
PRIMER_LEFT_3_SEQUENCE=CCTTACACTGCAAATGATCTGG
PRIMER_RIGHT_3_SEQUENCE=GAACCGTACCGTCCGTAACC
PRIMER_LEFT_3=36,22
PRIMER_RIGHT_3=203,20
PRIMER_LEFT_3_TM=59.632
PRIMER_RIGHT_3_TM=61.539
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=4.0404
PRIMER_LEFT_4_PENALTY=0.501869
PRIMER_RIGHT_4_PENALTY=3.538580
PRIMER_LEFT_4_SEQUENCE=TTACACTGCAAATGATCTGGGA
PRIMER_RIGHT_4_SEQUENCE=GAACCGTACCGTCCGTAACC
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=203,20
PRIMER_LEFT_4_TM=60.502
PRIMER_RIGHT_4_TM=61.539
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_00218 Ps_contig_mira-and-tgicl-ass_franssen_2553
SEQUENCE=AGAGCTCCTTCGAATAAGCAGAATATTTTATTCATACCTTACACTGCAAATGATCTGGGATGCCACGGTGACTCCTCGTGGTGCGTGTAAGCCAACTGCACGTCGTCCCCAACCTGCAGCGACACCGCCGACGCCTCCTCTCACAACTTACTTACAAGTAAACGTAACGTCCGTCCGTCGTTCCGGTTACGGACGGTACGGTTCGGTTCCGGTACCGGTACCGGTCCGTCCGACCCAACCGACCGTCCGTTTCCCGGTTACCGGGAACGGGAACGGAACCGACGGACGGACGTACTACGTA
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00219 Ps_contig_mira-and-tgicl-ass_franssen_2591
SEQUENCE=GAGTATAAATTTAGTTCTCTCGTATTCATTATTCATTTTCATTATTACGAATCTATCCATGGCTTCTTCTTTCACTTCGTTCCTTCGATTCAACAACCGNTGGATTTGATACTCTCAAGAACTTTGGTCAAATTTCTCCCGTTGTGAGGAATCATCTCAAACAGGTTTACTGACCTTGTGTTTGCGGTGCTGCTGCTGCTGTGGNTCTACT
TARGET=188,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00220 Ps_contig_mira-and-tgicl-ass_franssen_2592
SEQUENCE=GGACTTTGATGAAGAAAGAAAATTACAACTACCAGACTTCATTAGGGTTTTCTCCCAGACTTCATCCKATYWKMATKTTATTTACGAATCTATCCATGGCTTCTTCTTTCACTTCGTTCTTCGATTCAACAAACCGATGGAATTTTGATAACTACTCAAGAACTTCGGTCAAATTTCTCCCGTTGTGAGGAATCATCYCAAACAGGTTTACTTGACCTTGTGTTTTGCGGTTGCTGCTGCTGCTGTTGGACGTCCTTTACCTTCATTCGTTCCCTTCTTTCCACCGTTGGG
TARGET=232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00221 Ps_contig_mira-and-tgicl-ass_franssen_2593
SEQUENCE=GACACAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGACCCTTTAGGAATTGATCCAACAGGTATCCATTACCTGCGTGGATCTCTACTCCATCAAAACCTGCTTTCCATTGCATTTACCTTTTGCGGCAATTTTAAAATCATTGACAAT
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6728
PRIMER_LEFT_PENALTY=1.577079
PRIMER_RIGHT_PENALTY=0.095684
PRIMER_LEFT_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_SEQUENCE=AACATGCAACGGTGTAACTGAG
PRIMER_LEFT=8,21
PRIMER_RIGHT=142,22
PRIMER_LEFT_TM=60.577
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=1.6728
PRIMER_LEFT_1_PENALTY=1.577079
PRIMER_RIGHT_1_PENALTY=0.095684
PRIMER_LEFT_1_SEQUENCE=AAAAACACACCAAGCCAGTGA
PRIMER_RIGHT_1_SEQUENCE=AACATGCAACGGTGTAACTGAG
PRIMER_LEFT_1=7,21
PRIMER_RIGHT_1=142,22
PRIMER_LEFT_1_TM=60.577
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=1.7017
PRIMER_LEFT_2_PENALTY=1.577079
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_2_SEQUENCE=CAACTGCATGAAATGAGAGGAG
PRIMER_LEFT_2=8,21
PRIMER_RIGHT_2=119,22
PRIMER_LEFT_2_TM=60.577
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=1.7017
PRIMER_LEFT_3_PENALTY=1.577079
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=AAAAACACACCAAGCCAGTGA
PRIMER_RIGHT_3_SEQUENCE=CAACTGCATGAAATGAGAGGAG
PRIMER_LEFT_3=7,21
PRIMER_RIGHT_3=119,22
PRIMER_LEFT_3_TM=60.577
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=1.7116
PRIMER_LEFT_4_PENALTY=1.577079
PRIMER_RIGHT_4_PENALTY=0.134571
PRIMER_LEFT_4_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_4_SEQUENCE=CAATTCCTAAAGGGTCAAGTGG
PRIMER_LEFT_4=8,21
PRIMER_RIGHT_4=170,22
PRIMER_LEFT_4_TM=60.577
PRIMER_RIGHT_4_TM=59.865
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_00222 Ps_contig_mira-and-tgicl-ass_franssen_2593
SEQUENCE=GACACAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGACCCTTTAGGAATTGATCCAACAGGTATCCATTACCTGCGTGGATCTCTACTCCATCAAAACCTGCTTTCCATTGCATTTACCTTTTGCGGCAATTTTAAAATCATTGACAAT
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2959
PRIMER_LEFT_PENALTY=1.200250
PRIMER_RIGHT_PENALTY=0.095684
PRIMER_LEFT_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_SEQUENCE=AACATGCAACGGTGTAACTGAG
PRIMER_LEFT=9,21
PRIMER_RIGHT=142,22
PRIMER_LEFT_TM=60.200
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=1.3249
PRIMER_LEFT_1_PENALTY=1.200250
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_1_SEQUENCE=CAACTGCATGAAATGAGAGGAG
PRIMER_LEFT_1=9,21
PRIMER_RIGHT_1=119,22
PRIMER_LEFT_1_TM=60.200
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=1.3348
PRIMER_LEFT_2_PENALTY=1.200250
PRIMER_RIGHT_2_PENALTY=0.134571
PRIMER_LEFT_2_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_2_SEQUENCE=CAATTCCTAAAGGGTCAAGTGG
PRIMER_LEFT_2=9,21
PRIMER_RIGHT_2=170,22
PRIMER_LEFT_2_TM=60.200
PRIMER_RIGHT_2_TM=59.865
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=1.3363
PRIMER_LEFT_3_PENALTY=1.200250
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_3_SEQUENCE=TCCTAAAGGGTCAAGTGGATGT
PRIMER_LEFT_3=9,21
PRIMER_RIGHT_3=166,22
PRIMER_LEFT_3_TM=60.200
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=1.3770
PRIMER_LEFT_4_PENALTY=1.200250
PRIMER_RIGHT_4_PENALTY=0.176730
PRIMER_LEFT_4_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_4_SEQUENCE=TGTTGGATCAATTCCTAAAGGG
PRIMER_LEFT_4=9,21
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=60.200
PRIMER_RIGHT_4_TM=60.177
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_00223 Ps_contig_mira-and-tgicl-ass_franssen_2598
SEQUENCE=TAGTCGGGGAAGTCGCAGTTACTTCTGCGCAGTCTCTTTTTCGTTCGATTAGGGTTTTCTCTCAACAAGTTCTCTTCCGTTTTCAACCGACAATGAGGGCCAAGTGGARGAAGAAGCGTATGAGGAGGTTGAAGAGGAAGCGCCGAAAGATGAGACAGAGATCCAAGTAGTGTGTGTTCTCAGCCATACGTACGTACTAGCTCTTAGTTKTGTTTTTCCCTTTCGTGTTGTTTTGTGGTTATTTAATCAGACTCCTCAGCAAGAGAGTGGTATCTGTCTCTCTCTCTTTGCGCTGTTATGATATTGCGGGTTTGGTATTAGCTCAAAGATATTGATCTTTTTGGAATTGAGTAGCTATAACAGTTGGATTTCGCTCCTATTGTGTGGTTG
TARGET=277,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00224 Ps_contig_mira-and-tgicl-ass_franssen_2607
SEQUENCE=ACTTGGGGAGTGATGGGAAGTATGAGNGTTGGTTAAATGGGTTGATTGAAGAGCCACTTGGGAGGCCTGTGGAGAATGTTGATTCTGAGCTTGTTAAGATGTTTGAAGTGAATAATAATCAAGCCCAGCCCAGTGTTGATAATGGGCTTTCTACGCTCGGTTTGAGTGAGAATGGACCAACTACTGCTAGTGCTTGAGAATTGTTTTATTTCACAAATGAAATACATTACAATTTTTTTCTTCCATTTTTGCATKWTASTTATTWKTCTCTTASTTTTATTTATTTACTTTTACTTGNGTGTATAAGAGTTATTTTAGTTACGAANAAAGTATTAACTAAATTAATTTAACCTTTTCCCGTTGT
TARGET=276,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00225 Ps_contig_mira-and-tgicl-ass_franssen_2615
SEQUENCE=CGTTTTACGTTTAGTACTTACCTTAACTTAAGGTTAAAAGGGTTAAAAGGTTAAAGGGTAAAAAAAAACGAAAAAAAAACGAAAAAAGTTAAAAGTTACGACTTTAGTTTCTAACAAAAACTAAAAAAAAAAACTAGATAGACTTAACAAAGAACAAAAATTAAACTCATCAGAGAAGATCGGCGACATTTGAAGGCAATTCCTCTACGGTGACATTATAGAACTTCTGAATATCAGACAGCATTCTTGCATCATCCACAGTGACAAAGTTTATAGCAACACCTTTTCTTCCGAAACGCCCACTTCGCCCAATGCGATGGAGATAGTTTTCAGGTTGGGTAGGAAGATCATAGTTTATCACCAAAGACACTTGCTGAACATCAATACCACGAGCCAAAAGGTCGGTGGTAATGAGAACTCTGGAAGAACCAGAGCGGAATTCACGCATGATGATATCACGAGTGTTTTGGTCCATGTCTCCATGGGTGGCTGAGACTGTATGGTCCCTGCCTCGCATTTTGTCAGTGAGCCAGTCCACCTTGCGCCTTGTGTTCACAAAAATGACACTCTGAGTGATAGCCAGAGTCTCATAAAGGTCACATAATGTCTCTAGTTTCCATTCTTCCTTGTCAACATTCACATAAAACTGCTTGATACCCTCAAGGGTCAATTCATCACGCTTTACCAGAATTCTCACTGGCTTATTCATGAACTTCCTTGTGATCTCCAAGGCTTCTGGTGGCATTGTTGCAGAGAACACTCCAACCTGAATTTTAGGTGGCAGCAGCTGGAAGATATCATAGATCTGGTCCTTGAAACCACGTGAAAGCATCTCATCAGCCTCATCCAAAACAAACATTTTTATGCTATCGGACCGAAGAGACTGTCTTCGCAGCATGTCAAACACACGTCCAGGAGTGCCAACAACAGTGTGGACACCAGTTTGGAGAATACGCTGATCCTCGCGAACGCTAGTGCCACCAACACAAGCATGAACTTTAACACCAAGGTAATCACCAAGAGCTCTCATAACCTTCTCAATTTGCTGTGCAAGTTCCCTTGTTGGTGCCAAAACCAAAGCCTGGCACTGAACCAATCCATAATCGAGCTGCTGCAAAATTCCAGAACAAAAGGTTGCAGTCTTTCCAGTTCCAGACTGAGCCTGCTGAATCACATCTAGACCTTTGCAGAAAGGAACAATTCCCCTTTGCTGGATTGCAGAAGGCTTCTCAAAACCATAAGCATATATGCCTCTCAGAAGATTTTCTTGCAATCCCATAGCATCAAAAGTATCATAGACCTCATCATAAGTGGTGATGAGTTCTTGTCCATCAGTGGAAAGCAATTCATTCATCTTAGTGTCATACTGCTTAGCATCAAATTGTGAACCTTCCGGAGCCAAACCTGCCATACTGTTGGTAGAGGAAGAAAGAGAGAGAGACGCAAAGAGAGAGAGTGGTGGAAGAAGAAGAAGTGATTAGGGTTTCCCAAACTCCTCGGATCTGCGCCGTGGAGTTGAAGGGAAGAGTGAGAGAGAGAATTAGGTTAATATATATGTTTTTGATGTCAGTGTAGTATC
TARGET=1431,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1705
PRIMER_LEFT_PENALTY=0.026532
PRIMER_RIGHT_PENALTY=0.143960
PRIMER_LEFT_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT=1311,22
PRIMER_RIGHT=1532,22
PRIMER_LEFT_TM=59.973
PRIMER_RIGHT_TM=59.856
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.1705
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=0.143960
PRIMER_LEFT_1_SEQUENCE=GGTGATGAGTTCTTGTCCATCA
PRIMER_RIGHT_1_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_1=1312,22
PRIMER_RIGHT_1=1532,22
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.2022
PRIMER_LEFT_2_PENALTY=0.058242
PRIMER_RIGHT_2_PENALTY=0.143960
PRIMER_LEFT_2_SEQUENCE=GATTTTCTTGCAATCCCATAGC
PRIMER_RIGHT_2_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_2=1260,22
PRIMER_RIGHT_2=1532,22
PRIMER_LEFT_2_TM=59.942
PRIMER_RIGHT_2_TM=59.856
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.2190
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.143960
PRIMER_LEFT_3_SEQUENCE=CAGAAGATTTTCTTGCAATCCC
PRIMER_RIGHT_3_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_3=1255,22
PRIMER_RIGHT_3=1532,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=0.3056
PRIMER_LEFT_4_PENALTY=0.026532
PRIMER_RIGHT_4_PENALTY=0.279044
PRIMER_LEFT_4_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_4_SEQUENCE=GAGGAGTTTGGGAAACCCTAAT
PRIMER_LEFT_4=1311,22
PRIMER_RIGHT_4=1497,22
PRIMER_LEFT_4_TM=59.973
PRIMER_RIGHT_4_TM=59.721
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00226 Ps_contig_mira-and-tgicl-ass_franssen_2615
SEQUENCE=CGTTTTACGTTTAGTACTTACCTTAACTTAAGGTTAAAAGGGTTAAAAGGTTAAAGGGTAAAAAAAAACGAAAAAAAAACGAAAAAAGTTAAAAGTTACGACTTTAGTTTCTAACAAAAACTAAAAAAAAAAACTAGATAGACTTAACAAAGAACAAAAATTAAACTCATCAGAGAAGATCGGCGACATTTGAAGGCAATTCCTCTACGGTGACATTATAGAACTTCTGAATATCAGACAGCATTCTTGCATCATCCACAGTGACAAAGTTTATAGCAACACCTTTTCTTCCGAAACGCCCACTTCGCCCAATGCGATGGAGATAGTTTTCAGGTTGGGTAGGAAGATCATAGTTTATCACCAAAGACACTTGCTGAACATCAATACCACGAGCCAAAAGGTCGGTGGTAATGAGAACTCTGGAAGAACCAGAGCGGAATTCACGCATGATGATATCACGAGTGTTTTGGTCCATGTCTCCATGGGTGGCTGAGACTGTATGGTCCCTGCCTCGCATTTTGTCAGTGAGCCAGTCCACCTTGCGCCTTGTGTTCACAAAAATGACACTCTGAGTGATAGCCAGAGTCTCATAAAGGTCACATAATGTCTCTAGTTTCCATTCTTCCTTGTCAACATTCACATAAAACTGCTTGATACCCTCAAGGGTCAATTCATCACGCTTTACCAGAATTCTCACTGGCTTATTCATGAACTTCCTTGTGATCTCCAAGGCTTCTGGTGGCATTGTTGCAGAGAACACTCCAACCTGAATTTTAGGTGGCAGCAGCTGGAAGATATCATAGATCTGGTCCTTGAAACCACGTGAAAGCATCTCATCAGCCTCATCCAAAACAAACATTTTTATGCTATCGGACCGAAGAGACTGTCTTCGCAGCATGTCAAACACACGTCCAGGAGTGCCAACAACAGTGTGGACACCAGTTTGGAGAATACGCTGATCCTCGCGAACGCTAGTGCCACCAACACAAGCATGAACTTTAACACCAAGGTAATCACCAAGAGCTCTCATAACCTTCTCAATTTGCTGTGCAAGTTCCCTTGTTGGTGCCAAAACCAAAGCCTGGCACTGAACCAATCCATAATCGAGCTGCTGCAAAATTCCAGAACAAAAGGTTGCAGTCTTTCCAGTTCCAGACTGAGCCTGCTGAATCACATCTAGACCTTTGCAGAAAGGAACAATTCCCCTTTGCTGGATTGCAGAAGGCTTCTCAAAACCATAAGCATATATGCCTCTCAGAAGATTTTCTTGCAATCCCATAGCATCAAAAGTATCATAGACCTCATCATAAGTGGTGATGAGTTCTTGTCCATCAGTGGAAAGCAATTCATTCATCTTAGTGTCATACTGCTTAGCATCAAATTGTGAACCTTCCGGAGCCAAACCTGCCATACTGTTGGTAGAGGAAGAAAGAGAGAGAGACGCAAAGAGAGAGAGTGGTGGAAGAAGAAGAAGTGATTAGGGTTTCCCAAACTCCTCGGATCTGCGCCGTGGAGTTGAAGGGAAGAGTGAGAGAGAGAATTAGGTTAATATATATGTTTTTGATGTCAGTGTAGTATC
TARGET=1447,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1705
PRIMER_LEFT_PENALTY=0.026532
PRIMER_RIGHT_PENALTY=0.143960
PRIMER_LEFT_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT=1311,22
PRIMER_RIGHT=1532,22
PRIMER_LEFT_TM=59.973
PRIMER_RIGHT_TM=59.856
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.1705
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=0.143960
PRIMER_LEFT_1_SEQUENCE=GGTGATGAGTTCTTGTCCATCA
PRIMER_RIGHT_1_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_1=1312,22
PRIMER_RIGHT_1=1532,22
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.2022
PRIMER_LEFT_2_PENALTY=0.058242
PRIMER_RIGHT_2_PENALTY=0.143960
PRIMER_LEFT_2_SEQUENCE=GATTTTCTTGCAATCCCATAGC
PRIMER_RIGHT_2_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_2=1260,22
PRIMER_RIGHT_2=1532,22
PRIMER_LEFT_2_TM=59.942
PRIMER_RIGHT_2_TM=59.856
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.2190
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.143960
PRIMER_LEFT_3_SEQUENCE=CAGAAGATTTTCTTGCAATCCC
PRIMER_RIGHT_3_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_3=1255,22
PRIMER_RIGHT_3=1532,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=0.3056
PRIMER_LEFT_4_PENALTY=0.026532
PRIMER_RIGHT_4_PENALTY=0.279044
PRIMER_LEFT_4_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_4_SEQUENCE=GAGGAGTTTGGGAAACCCTAAT
PRIMER_LEFT_4=1311,22
PRIMER_RIGHT_4=1497,22
PRIMER_LEFT_4_TM=59.973
PRIMER_RIGHT_4_TM=59.721
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00227 Ps_contig_mira-and-tgicl-ass_franssen_2615
SEQUENCE=CGTTTTACGTTTAGTACTTACCTTAACTTAAGGTTAAAAGGGTTAAAAGGTTAAAGGGTAAAAAAAAACGAAAAAAAAACGAAAAAAGTTAAAAGTTACGACTTTAGTTTCTAACAAAAACTAAAAAAAAAAACTAGATAGACTTAACAAAGAACAAAAATTAAACTCATCAGAGAAGATCGGCGACATTTGAAGGCAATTCCTCTACGGTGACATTATAGAACTTCTGAATATCAGACAGCATTCTTGCATCATCCACAGTGACAAAGTTTATAGCAACACCTTTTCTTCCGAAACGCCCACTTCGCCCAATGCGATGGAGATAGTTTTCAGGTTGGGTAGGAAGATCATAGTTTATCACCAAAGACACTTGCTGAACATCAATACCACGAGCCAAAAGGTCGGTGGTAATGAGAACTCTGGAAGAACCAGAGCGGAATTCACGCATGATGATATCACGAGTGTTTTGGTCCATGTCTCCATGGGTGGCTGAGACTGTATGGTCCCTGCCTCGCATTTTGTCAGTGAGCCAGTCCACCTTGCGCCTTGTGTTCACAAAAATGACACTCTGAGTGATAGCCAGAGTCTCATAAAGGTCACATAATGTCTCTAGTTTCCATTCTTCCTTGTCAACATTCACATAAAACTGCTTGATACCCTCAAGGGTCAATTCATCACGCTTTACCAGAATTCTCACTGGCTTATTCATGAACTTCCTTGTGATCTCCAAGGCTTCTGGTGGCATTGTTGCAGAGAACACTCCAACCTGAATTTTAGGTGGCAGCAGCTGGAAGATATCATAGATCTGGTCCTTGAAACCACGTGAAAGCATCTCATCAGCCTCATCCAAAACAAACATTTTTATGCTATCGGACCGAAGAGACTGTCTTCGCAGCATGTCAAACACACGTCCAGGAGTGCCAACAACAGTGTGGACACCAGTTTGGAGAATACGCTGATCCTCGCGAACGCTAGTGCCACCAACACAAGCATGAACTTTAACACCAAGGTAATCACCAAGAGCTCTCATAACCTTCTCAATTTGCTGTGCAAGTTCCCTTGTTGGTGCCAAAACCAAAGCCTGGCACTGAACCAATCCATAATCGAGCTGCTGCAAAATTCCAGAACAAAAGGTTGCAGTCTTTCCAGTTCCAGACTGAGCCTGCTGAATCACATCTAGACCTTTGCAGAAAGGAACAATTCCCCTTTGCTGGATTGCAGAAGGCTTCTCAAAACCATAAGCATATATGCCTCTCAGAAGATTTTCTTGCAATCCCATAGCATCAAAAGTATCATAGACCTCATCATAAGTGGTGATGAGTTCTTGTCCATCAGTGGAAAGCAATTCATTCATCTTAGTGTCATACTGCTTAGCATCAAATTGTGAACCTTCCGGAGCCAAACCTGCCATACTGTTGGTAGAGGAAGAAAGAGAGAGAGACGCAAAGAGAGAGAGTGGTGGAAGAAGAAGAAGTGATTAGGGTTTCCCAAACTCCTCGGATCTGCGCCGTGGAGTTGAAGGGAAGAGTGAGAGAGAGAATTAGGTTAATATATATGTTTTTGATGTCAGTGTAGTATC
TARGET=1462,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1705
PRIMER_LEFT_PENALTY=0.026532
PRIMER_RIGHT_PENALTY=0.143960
PRIMER_LEFT_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT=1311,22
PRIMER_RIGHT=1532,22
PRIMER_LEFT_TM=59.973
PRIMER_RIGHT_TM=59.856
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.1705
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=0.143960
PRIMER_LEFT_1_SEQUENCE=GGTGATGAGTTCTTGTCCATCA
PRIMER_RIGHT_1_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_1=1312,22
PRIMER_RIGHT_1=1532,22
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.2022
PRIMER_LEFT_2_PENALTY=0.058242
PRIMER_RIGHT_2_PENALTY=0.143960
PRIMER_LEFT_2_SEQUENCE=GATTTTCTTGCAATCCCATAGC
PRIMER_RIGHT_2_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_2=1260,22
PRIMER_RIGHT_2=1532,22
PRIMER_LEFT_2_TM=59.942
PRIMER_RIGHT_2_TM=59.856
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.2190
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.143960
PRIMER_LEFT_3_SEQUENCE=CAGAAGATTTTCTTGCAATCCC
PRIMER_RIGHT_3_SEQUENCE=TCTCACTCTTCCCTTCAACTCC
PRIMER_LEFT_3=1255,22
PRIMER_RIGHT_3=1532,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=0.3056
PRIMER_LEFT_4_PENALTY=0.026532
PRIMER_RIGHT_4_PENALTY=0.279044
PRIMER_LEFT_4_SEQUENCE=TGGTGATGAGTTCTTGTCCATC
PRIMER_RIGHT_4_SEQUENCE=GAGGAGTTTGGGAAACCCTAAT
PRIMER_LEFT_4=1311,22
PRIMER_RIGHT_4=1497,22
PRIMER_LEFT_4_TM=59.973
PRIMER_RIGHT_4_TM=59.721
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00228 Ps_contig_mira-and-tgicl-ass_franssen_2615
SEQUENCE=CGTTTTACGTTTAGTACTTACCTTAACTTAAGGTTAAAAGGGTTAAAAGGTTAAAGGGTAAAAAAAAACGAAAAAAAAACGAAAAAAGTTAAAAGTTACGACTTTAGTTTCTAACAAAAACTAAAAAAAAAAACTAGATAGACTTAACAAAGAACAAAAATTAAACTCATCAGAGAAGATCGGCGACATTTGAAGGCAATTCCTCTACGGTGACATTATAGAACTTCTGAATATCAGACAGCATTCTTGCATCATCCACAGTGACAAAGTTTATAGCAACACCTTTTCTTCCGAAACGCCCACTTCGCCCAATGCGATGGAGATAGTTTTCAGGTTGGGTAGGAAGATCATAGTTTATCACCAAAGACACTTGCTGAACATCAATACCACGAGCCAAAAGGTCGGTGGTAATGAGAACTCTGGAAGAACCAGAGCGGAATTCACGCATGATGATATCACGAGTGTTTTGGTCCATGTCTCCATGGGTGGCTGAGACTGTATGGTCCCTGCCTCGCATTTTGTCAGTGAGCCAGTCCACCTTGCGCCTTGTGTTCACAAAAATGACACTCTGAGTGATAGCCAGAGTCTCATAAAGGTCACATAATGTCTCTAGTTTCCATTCTTCCTTGTCAACATTCACATAAAACTGCTTGATACCCTCAAGGGTCAATTCATCACGCTTTACCAGAATTCTCACTGGCTTATTCATGAACTTCCTTGTGATCTCCAAGGCTTCTGGTGGCATTGTTGCAGAGAACACTCCAACCTGAATTTTAGGTGGCAGCAGCTGGAAGATATCATAGATCTGGTCCTTGAAACCACGTGAAAGCATCTCATCAGCCTCATCCAAAACAAACATTTTTATGCTATCGGACCGAAGAGACTGTCTTCGCAGCATGTCAAACACACGTCCAGGAGTGCCAACAACAGTGTGGACACCAGTTTGGAGAATACGCTGATCCTCGCGAACGCTAGTGCCACCAACACAAGCATGAACTTTAACACCAAGGTAATCACCAAGAGCTCTCATAACCTTCTCAATTTGCTGTGCAAGTTCCCTTGTTGGTGCCAAAACCAAAGCCTGGCACTGAACCAATCCATAATCGAGCTGCTGCAAAATTCCAGAACAAAAGGTTGCAGTCTTTCCAGTTCCAGACTGAGCCTGCTGAATCACATCTAGACCTTTGCAGAAAGGAACAATTCCCCTTTGCTGGATTGCAGAAGGCTTCTCAAAACCATAAGCATATATGCCTCTCAGAAGATTTTCTTGCAATCCCATAGCATCAAAAGTATCATAGACCTCATCATAAGTGGTGATGAGTTCTTGTCCATCAGTGGAAAGCAATTCATTCATCTTAGTGTCATACTGCTTAGCATCAAATTGTGAACCTTCCGGAGCCAAACCTGCCATACTGTTGGTAGAGGAAGAAAGAGAGAGAGACGCAAAGAGAGAGAGTGGTGGAAGAAGAAGAAGTGATTAGGGTTTCCCAAACTCCTCGGATCTGCGCCGTGGAGTTGAAGGGAAGAGTGAGAGAGAGAATTAGGTTAATATATATGTTTTTGATGTCAGTGTAGTATC
TARGET=1530,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00229 Ps_contig_mira-and-tgicl-ass_franssen_2622
SEQUENCE=GGCGGGGGGGTCTTAGGCCCAAATAATCCACCCTCCCCATACGACGTCGTAGATAAAACTCACATTCCACCGTAATCATCGATCTGAGAACAGAAACTGAAAAAGAGAGAGTGGGAAAACACTTTCAATTTCTTCATCTTCTTCTTCTTGTAATAATAAAACCAGAAAATCGAAACGAAACCGAAATGGGCAAGGATCTGAGCGACGATCAAATTTCTTCCATGAAAGAAGCTTTCACTCTATTCGACACCGACGGCGACGGCAAGATCGCTCCATCGGAGCTCGGAATCCTCATGCGATCTCTCGGCCGGAAACCCNCCAAGCTCAACTCAAT
TARGET=137,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7696
PRIMER_LEFT_PENALTY=0.147734
PRIMER_RIGHT_PENALTY=0.621889
PRIMER_LEFT_SEQUENCE=AAATAATCCACCCTCCCCATAC
PRIMER_RIGHT_SEQUENCE=TCGGTGTCGAATAGAGTGAAAG
PRIMER_LEFT=20,22
PRIMER_RIGHT=252,22
PRIMER_LEFT_TM=60.148
PRIMER_RIGHT_TM=59.378
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.9054
PRIMER_LEFT_1_PENALTY=0.147734
PRIMER_RIGHT_1_PENALTY=0.757660
PRIMER_LEFT_1_SEQUENCE=AAATAATCCACCCTCCCCATAC
PRIMER_RIGHT_1_SEQUENCE=GTCGGTGTCGAATAGAGTGAAA
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=253,22
PRIMER_LEFT_1_TM=60.148
PRIMER_RIGHT_1_TM=59.242
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.9288
PRIMER_LEFT_2_PENALTY=0.306868
PRIMER_RIGHT_2_PENALTY=0.621889
PRIMER_LEFT_2_SEQUENCE=ATTCCACCGTAATCATCGATCT
PRIMER_RIGHT_2_SEQUENCE=TCGGTGTCGAATAGAGTGAAAG
PRIMER_LEFT_2=63,22
PRIMER_RIGHT_2=252,22
PRIMER_LEFT_2_TM=59.693
PRIMER_RIGHT_2_TM=59.378
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=1.0645
PRIMER_LEFT_3_PENALTY=0.306868
PRIMER_RIGHT_3_PENALTY=0.757660
PRIMER_LEFT_3_SEQUENCE=ATTCCACCGTAATCATCGATCT
PRIMER_RIGHT_3_SEQUENCE=GTCGGTGTCGAATAGAGTGAAA
PRIMER_LEFT_3=63,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=59.693
PRIMER_RIGHT_3_TM=59.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=1.2627
PRIMER_LEFT_4_PENALTY=0.640802
PRIMER_RIGHT_4_PENALTY=0.621889
PRIMER_LEFT_4_SEQUENCE=AACTCACATTCCACCGTAATCA
PRIMER_RIGHT_4_SEQUENCE=TCGGTGTCGAATAGAGTGAAAG
PRIMER_LEFT_4=56,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=59.359
PRIMER_RIGHT_4_TM=59.378
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=197
=
PRIMER_SEQUENCE_ID=SSR_00230 Ps_contig_mira-and-tgicl-ass_franssen_2626
SEQUENCE=CGGGGGTTATTTTGTAAATACAGTTTGGGTTCGTAAAAAAGCGCTTATTCCAAACGCGATCTGTGGTGGTGGTGGTGCTGCAGAAACATCAAGGCAAAACCAAAACCCCCATTCACACACCCGCTAGGGTATCTTTCTTCTGCTAAACCAAACCAAATGGCTGATCCTGAGCTGGAAGCTATCAGACAGAGAAGGATGCGGAGGAGCTTAGTGGGGTCGGCATGGCGAACGGGAAATCCAACCGAACCTGAACAGCAGAGCGCTCGAGGGAG
TARGET=63,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.6195
PRIMER_LEFT_PENALTY=2.615161
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=AAAAGCGCTTATTCCAAACG
PRIMER_RIGHT_SEQUENCE=CTGATAGCTTCCAGCTCAGGAT
PRIMER_LEFT=36,20
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=59.385
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=2.6195
PRIMER_LEFT_1_PENALTY=2.615161
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=AAAAGCGCTTATTCCAAACG
PRIMER_RIGHT_1_SEQUENCE=ATAGCTTCCAGCTCAGGATCAG
PRIMER_LEFT_1=36,20
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=59.385
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=2.6806
PRIMER_LEFT_2_PENALTY=2.676221
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=GCTTATTCCAAACGCGATCT
PRIMER_RIGHT_2_SEQUENCE=ATAGCTTCCAGCTCAGGATCAG
PRIMER_LEFT_2=42,20
PRIMER_RIGHT_2=181,22
PRIMER_LEFT_2_TM=59.324
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=2.6806
PRIMER_LEFT_3_PENALTY=2.676221
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=GCTTATTCCAAACGCGATCT
PRIMER_RIGHT_3_SEQUENCE=CTGATAGCTTCCAGCTCAGGAT
PRIMER_LEFT_3=42,20
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=59.324
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=2.7233
PRIMER_LEFT_4_PENALTY=2.615161
PRIMER_RIGHT_4_PENALTY=0.108177
PRIMER_LEFT_4_SEQUENCE=AAAAGCGCTTATTCCAAACG
PRIMER_RIGHT_4_SEQUENCE=AGAAGAAAGATACCCTAGCGGG
PRIMER_LEFT_4=36,20
PRIMER_RIGHT_4=140,22
PRIMER_LEFT_4_TM=59.385
PRIMER_RIGHT_4_TM=60.108
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=12.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00231 Ps_contig_mira-and-tgicl-ass_franssen_2627
SEQUENCE=TTCTTACTTAATTGTTCTTGGAGATTCTGATGATGTACTTGAGCATTTGGAGTGATTCTTGTATTTTAATTGTGATATGTGATACAAAACTTTGATACTTTCCCCCTTGAGCCATGTATTATGTACACTACCAAGGCTTTCTTTTGGTCTTGTGTTAATATATTGTAGACATTTATGAAGTCTAAGTTTTWRWAAMSTCTATTCATTGTCTATGTTATTTTTATTTACAACAGACCAGAGATATACAACGTGGAATAAGTGTAATATATAAATAAATAAAACTAAAAATT
TARGET=268,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00232 Ps_contig_mira-and-tgicl-ass_franssen_2637
SEQUENCE=GTAGTGAAAACAAAAACTCAAGCTTTTGAGGTCACTTATTTTCGTTCCTAGTAATTCGGATATGGTGGTTCTCAGAGTTTTGGGTCTTTGGAATGGAGTGATGGAGTTCATATTGTGAGGAGCCCAATTGCTGCTAGATGGAGCAATGGATTCTCATCAGCATTCCYTTTTGTAWTTTTTCATTCTGTAGAAAGTGGAAACACATTATTATTATTACCATGCATAGGTAGGAAACATTGTTATGTATGTAAAGTTAATTAGTTCTTGTTAATTAGTTAAGTTAAACTTTTAATTAGTTAGTTACTTTTAATTAGTAAACAAAACTAAAAACAAAGTAAAA
TARGET=204,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00233 Ps_contig_mira-and-tgicl-ass_franssen_2638
SEQUENCE=AGTTAAACAAGAAACAAACCAAACCAAATAATTATTCTTGTTTAGTTTGTTACAATAAAAGACTAACATAAAAGTTAACAACAATACAAGACTAATTAACTTTCATACATAACATGTTTCCACCTATGCATGGTAATAATAATAATGTGTTTCCACTTTCACAGAATGAAAAATCAAAAGGATGCTGATGAGAATCCATTGCCTCCATCTAGCAGCAAGTTGGGGCCTCCCTACAACAATATKMR
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00234 Ps_contig_mira-and-tgicl-ass_franssen_2639
SEQUENCE=TTTGGGTTCTTTGGAATGGAGTGATGGAGTTCATATTGTGAGGAGCCCAATTGCTGCTAGATGGAGCAATGGATTCTCATCAGCATYCYTTTGTATTTTTCATTCTGTGAAGTGGAAACACATTATTATTATTACCATGCAAKTAGGGTAGGAAAMMTWGTTATGTATNAAGTTTAATTAGTTCTTTAGTTATTTAGTTTAGTTTAAAC
TARGET=122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00235 Ps_contig_mira-and-tgicl-ass_franssen_2686
SEQUENCE=GCATAAGTTTCTAGCGACGTATCCATCCAATTACTAAACTTGGTCGACCTCCTCAATCTTGGGACCAGGGCCGCCACTGGCACTTGTAGGTGGAGGAACTTCATCATCATCACCACCCATGTCAGGAGCAGCTCCACCTTGGTACATCTTAGCAATTATTGGGTTGCAAAGACTCTCAAGTTCCCTCATCTTATCTTCAAACTCATCTGCTTCAGCCAACTGGTTATTATCCAACCAAGTAATTGAAGCATCAATTGCATCTTCAATTCTTTTTCTTTGTCATCAGCCGAAAGCTTTGAAGCAATTCTTTTCATCCTTAATCGTGTTCCTCATGTTGTAAGCATTAGTTTTCCAGTGCTATTTCTTTGCACTACCTAACCTTTTCCTTTCTTTAGGTTTCGTTTCCCCTC
TARGET=101,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2122
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.205226
PRIMER_LEFT_SEQUENCE=GTTTCTAGCGACGTATCCATCC
PRIMER_RIGHT_SEQUENCE=AACTTGAGAGTCTTTGCAACCC
PRIMER_LEFT=6,22
PRIMER_RIGHT=181,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.795
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.2276
PRIMER_LEFT_1_PENALTY=0.006951
PRIMER_RIGHT_1_PENALTY=0.220659
PRIMER_LEFT_1_SEQUENCE=GTTTCTAGCGACGTATCCATCC
PRIMER_RIGHT_1_SEQUENCE=TAATAACCAGTTGGCTGAAGCA
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=228,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=59.779
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.2604
PRIMER_LEFT_2_PENALTY=0.006951
PRIMER_RIGHT_2_PENALTY=0.253482
PRIMER_LEFT_2_SEQUENCE=GTTTCTAGCGACGTATCCATCC
PRIMER_RIGHT_2_SEQUENCE=TGAGAGTCTTTGCAACCCAATA
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=177,22
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=59.747
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=0.3929
PRIMER_LEFT_3_PENALTY=0.006951
PRIMER_RIGHT_3_PENALTY=0.385922
PRIMER_LEFT_3_SEQUENCE=GTTTCTAGCGACGTATCCATCC
PRIMER_RIGHT_3_SEQUENCE=GGTTGGATAATAACCAGTTGGC
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=235,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=59.614
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=0.4365
PRIMER_LEFT_4_PENALTY=0.231280
PRIMER_RIGHT_4_PENALTY=0.205226
PRIMER_LEFT_4_SEQUENCE=AGCGACGTATCCATCCAATTAC
PRIMER_RIGHT_4_SEQUENCE=AACTTGAGAGTCTTTGCAACCC
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=181,22
PRIMER_LEFT_4_TM=60.231
PRIMER_RIGHT_4_TM=59.795
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_00236 Ps_contig_mira-and-tgicl-ass_franssen_2691
SEQUENCE=ACTTTTTTTAACGTTTTAGTCAATNTTACATTACTTTATTGTAAATCAAAATTAACTTGGAACTTTAATTTTTTTATTTTCTAATTACAAATAAAATTAATAATCTTTAAAGAATCCAAAGTAAATAGCAAGAAATAACACAGGTCTGGGAAGGAGGAGAAAAATTGAAGAAATGTATCTCACATACATACATGTAACAGCTTTATTTCTATATCTCGAACAAAAATAGGGTATGATTTCCGGCTAGTCTCTCATCGAACTTGGCGCGATAAGATTTGACATAGTTATTGGCAATAATTATCTTCTTTCCAAAAATGAAAAATGTAATTTGAAAGCAGCTAGTTGACAACATCAAGAACCAACTTGCGAGACTTCAGAATGGACCATCGAAGCCGACGATAATATGCGGCTTGAAGTAATGCCAATGATAGTACAAATATCCATTTAAATCCCATCAGTTTTCTCTCTTCCATCTCCGGTTCTGCAGCCCAAGACAAAAATGACACAACATCTTTCCCCATCTGAGATTCTGTAGCAGGGGTACCATCTTCATATTCAACAGCACCATCATTAAGCATTTTAGGCATGGCAATGGCTCCGCCTMGG
TARGET=182,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00237 Ps_contig_mira-and-tgicl-ass_franssen_2702
SEQUENCE=TTTTATGCATATATATATATATAAAAGACAAGACTATGTTCTATTTAATGTCTTAAGACTGTTTTTCAGGTGTACGTATGTTTATTGTTACGAGCATATATATATGATTATTCTTAAGAATAACT
TARGET=9,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00238 Ps_contig_mira-and-tgicl-ass_franssen_2702
SEQUENCE=TTTTATGCATATATATATATATAAAAGACAAGACTATGTTCTATTTAATGTCTTAAGACTGTTTTTCAGGTGTACGTATGTTTATTGTTACGAGCATATATATATGATTATTCTTAAGAATAACT
TARGET=96,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00239 Ps_contig_mira-and-tgicl-ass_franssen_2741
SEQUENCE=GGAAAAGAAAAAGAAAAGTAAAAAGWSGGAGTACTAGTGACCGGTGAAGCAGAGGGAAAATTGAAGAGAAAGAAAACAGTTGATTCCGACGAAAAAGAGTGAAATCCAAGCTTCAAATTTACGATGGATGTGATCAAAACGCAGCAAATCTCATCTCGATCGATCGAGAAGGTGGTGGTTCATCCATTAGTTTTGCTCAGCATCGTCGACAACTACAACAGAGTCGCCAAGGACACACGGAAGCGCGTCGTCGGCGTCTTGCTAGGTTCCACTTTCAAAGGCACCGTCGACGTTTCCAACAGCTACGCAGTGCCCTTTGAAGAAGATGACAAGGATCCTAGCATTTGGTTTCTCGACCACAATTACCATGAAGCAATGTTTTCCATGTTTAAGAGAATAAACGCAAAGGAGCACGTCGTGGGGTGGTATAGCACAGGTCCAAAATTGCGTGAAAATGACCTCGACATTCATGGGTTATTTAATGACTATGTTCCAAATCCAGTGTTGGTTATAATTGATGTTGAACCTAAGGAGTTGGGAATTCCAACAAAAGCATACTATGCTGTTGAGGAAGTTAAAGAGAATGCTACTCAGAAAAGCCAAAAGGTCTTTGTGCATGTGCAATCCGAGATAGCTGCTCATGAGGTTGAGGAAATAGGAGTGGAACACTTGCTTAGGGATGTAAAGGATACAACCATCAGCACCCTTGCAACCGAGGTGAGTGGGAAACTCACAGCCTTGAAAGGTTTGGATGCAAGGCTTAAAGAGATAAGGAGTTACCTTGATCTTGTAATTGATGAAAAGCTTCCCTTAAACCATGAAATCCTATACCATCTTCAGGATGTGTTCAACCTGCTACCAAATCTTAATGTGTCTGATCTTATCAAGGCTTTTGCAGTGAAAACCAATGATATGATGCTGGTAATATACCTATCATCTCTCATTAGAAGTGTAATTGCACTCCACAACTTGATTAACAACAAGATGCTCAACAAAGAACATGAAAGGGCAGAAGACTCAAAATCTGTACCGGTGCCAAGTGCATCTGCGTAAGGTTTGAGGGTTGTAATGGTTTGAGCTCTAAAGGCACCGTGATTGATTCCATGGACAGATTCTTACAACTTAAATTGCTTTTGTCAGCCCTCAAGCATTTCTGATAGGGTAGAGAAATACATGTAACCTAGCATAACGATCCTTTTGCATCATTTATGTATTTCACTCTTCTATCCTATGATCATATCCTTAGTTTAGTATCTTTGTTAACATCTGTTGAATAACTTGTTAAAAACGAATTGATCACCGATATCAGGAATCAAATGTCTCCTTAATTGTTTTTTATAATATTTATCACTTGCCCAAT
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00240 Ps_contig_mira-and-tgicl-ass_franssen_2747
SEQUENCE=CTAAACAAATAAAAAATAAATAGTACCAGACAAGAAATAACCAAAAACGTACCGGGACAACTACCAGTTCTAAGAATAAGAAAAAAAAGTAACAAAAAGGTGGACACAATCCATGAAATTKTTAAGATATTAAACTCTCTTCTCAATCTAATTCAATCAAGCATGTCTGTTCCTCTTCTTGGCACCAGACTTTGCAACTTTGAGGCTTCTGTTAACAACACTCAACCTTGCAAGAGCAGCCTTCTTGAGATCTGGTCTGTAGTAGTTATCTGCCACCTGGTTCTGAACAGCCTTAGCCATTCTCCTAAACTCTTTCTTCATCACAGATTTGTGAAGCAAAGCCGAAGGCTTGTTCTGCTTCCTTGGCTTGGAGGTGGCCAACAACACAGACTGATCCTTCCCAGCTGACTGAATAGTAACAGTTTTCTTGTTGGCCAAACCAGAGTACTTGTAGGAGTTGAGGTTGTAGAGATTGTTTGGCTCTCTGCTAAACTGAACACTCTGAGTGTTGTTACCAAACTCCTTGACCAAGAAAGAGTTGTTCCTCTTCACAATCTCCCAAACAAGAGGTCCAGGTACTGTTGTCATCTTCTTCTTCTACGAGCAAACCCTTTGGCTGTTGTGAGAGAAATGCGATTTGAGAAGAAAGAGTAAGCCGCAAAGCAATATATGTAGAGAGATCCCCGACGTACTCTGCGTTG
TARGET=588,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00241 Ps_contig_mira-and-tgicl-ass_franssen_2748
SEQUENCE=TCTGGTCTGTAGTAGTTATCTGCCACCTTTGGTTTTCTTTGKTAACTWAGTCCTTAGTCCATTCTCCTAAACTCTTTCTTCATCACAGATTTGTGAAGCAAAGCCGAAGGCTTGTTCTGCTTCCTTGGCTTGGAGGTGGCCAACAACACAGACTGATCCTTCCCAGCTGACTGAATAGTAACAGTTTTCTTGTTGGCCAAACCAGAGTACTTGTAGGAGTTGAGGTTGTAGAGATTGTTTGGCTCTCTGCTAAACTGAACACTCTGAGTTGTTGTTACCAAACTCCTTGACCAAGAAAGAGTTGTTCCTCTTCACAACTMMWCCWAANAAGGACGGTCCGTAACGGTTACTTGGTTTCGTACTACTTTCCTTTCCTTTCCTTACCTAACGACGAACCCTTAAACCCGTTTGGTCGTTGGTTTGGTAGGAAGGAAAGTAAAACGTCGGTACCGTTTACCCCG
TARGET=365,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00242 Ps_contig_mira-and-tgicl-ass_franssen_2764
SEQUENCE=ACATGGTATCAACGSRGRTATCATCATTATGCAGCCTTATCGTTAACACTATTTCTCTCCGTTTTCTGAAATTACCTTTTCACAACAATGAATTCATCATCGCTACTTCGTCCTTCTCCTCCTCCTCTTCTTTTTCATCGCAACCGTTTCGTTATTTCTTCTTCATCTAGAAACCAATCTTACATCCCTAAACTCGAACCTTTTAGCCGATCCAAAATCGACAGAGTCGCTAAAAGCCTCCCTATCATTAGAAAAATCTAGAAAAAGATCTCTCCGATTATTGTTCTACACTTGAAGGTGATGAATGTTATAGTTGCTGGCAGGCTTATTTTGAGCTACAAAGATACTTACAAAAGGNTCACCGAGAGGAGATATAGAGAGGTTAATTATTCAGATTGGTGGTAGTAAATCCTTGATAGGATTAGTTTACAGTTAGGTAATTGAC
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00243 Ps_contig_mira-and-tgicl-ass_franssen_2793
SEQUENCE=TAAGATTTYTTTTYTTAACTWAATACAATAGATCATATTATAACAATCTTGTTTGTAAACAAAAAGTAAATACGCTAAATTCAAAATACAATCTTGTACCAGTACATAAAAGAACAAAAATAAAACAAACTGCATAACAGGTAAAACAAAATAACTACAACAAAAAACAAACTAGGAAAAATGCAACCCACGTCAGAGTTTGCCGGGAATGAAGTTTTGTTAAAACACAATTATCTTCAGTAAGCTCTTAATGTGCTAACTGATAAAACAAGCAACTCCAATATGAGAAAGGAAATGTGATTACTGAAGAAAGAGGAAAACGAGGAAGTAACTGCTGCTGCTGAACTCCTTGTGAAATTGAGTGTTGA
TARGET=332,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00244 Ps_contig_mira-and-tgicl-ass_franssen_2817
SEQUENCE=GGGGTATCAAGATCCAACGGTCCATAATGTACGAGCGTTCTTACTCCTATTTTTTCAGAGGTTTTAGTGCTTCAAACCCCAGATCTTGCTTATCAGTCTCTAGATCTTCGTTCCTGGAATTAAAAATTTTCTGGTTTGAAGAAAAGCTTGTTTGTTTGTCACACGAATGGAGACTGAACACTAGTTCATNATGCACGCGCGGCGACTTACTGC
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2774
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=1.276629
PRIMER_LEFT_SEQUENCE=TTTAGTGCTTCAAACCCCAGAT
PRIMER_RIGHT_SEQUENCE=AGTGTTCAGTCTCCATTCGTGT
PRIMER_LEFT=62,22
PRIMER_RIGHT=181,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=58.723
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=1.3107
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=1.276629
PRIMER_LEFT_1_SEQUENCE=CAAACCCCAGATCTTGCTTATC
PRIMER_RIGHT_1_SEQUENCE=AGTGTTCAGTCTCCATTCGTGT
PRIMER_LEFT_1=72,22
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=58.723
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=110
PRIMER_PAIR_PENALTY_2=1.3107
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=1.276629
PRIMER_LEFT_2_SEQUENCE=AAACCCCAGATCTTGCTTATCA
PRIMER_RIGHT_2_SEQUENCE=AGTGTTCAGTCTCCATTCGTGT
PRIMER_LEFT_2=73,22
PRIMER_RIGHT_2=181,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=58.723
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=1.3117
PRIMER_LEFT_3_PENALTY=0.035021
PRIMER_RIGHT_3_PENALTY=1.276629
PRIMER_LEFT_3_SEQUENCE=GTTTTAGTGCTTCAAACCCCAG
PRIMER_RIGHT_3_SEQUENCE=AGTGTTCAGTCTCCATTCGTGT
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=181,22
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=58.723
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=1.3581
PRIMER_LEFT_4_PENALTY=0.081468
PRIMER_RIGHT_4_PENALTY=1.276629
PRIMER_LEFT_4_SEQUENCE=GTATCAAGATCCAACGGTCCAT
PRIMER_RIGHT_4_SEQUENCE=AGTGTTCAGTCTCCATTCGTGT
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=181,22
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=58.723
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_00245 Ps_contig_mira-and-tgicl-ass_franssen_2829
SEQUENCE=TGAAATATTCCCCGTCCCAAATTGATCATCCTTCCCAACAGCAGGAACAGGCGGCCCTTCCATCCCCGAACCCGTTGTCCATCCTCTACCAGTTGCAGCACTCAACACCAGCCCCATCGCAGCATCATTGGAAAGAGGGGAAATGGAGAAACAGCGGTTACTGGGAACTGGAAGGTTGATGTTGAGGTTGATCTCGTCGGAATCGTTGGCATCGTCACGTTTGGAGGAGGGTAGACGAAAGTGGTAGGTTTTCTGGAAAGGAAARGGKGATSKTRTTCGTTTGGTTTGGTTTGGTTTGGTTTAGGAAAGGGGAAGGAACGGGAAGGAAAAGGAAAGGGTAGTCGGTTTGTTAGGAAAGAAGATGGTAAAGGTTAAAG
TARGET=279,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00246 Ps_contig_mira-and-tgicl-ass_franssen_2834
SEQUENCE=TGGATCTAGGTTTTCTAAAATTTTGTTAGGTTTTCTTCAATTTTAAGTTAAGGATTCACCGATTTTGTGGTTAGATTTTATTGAATTGAATTGATTGAAAATTTGGACTTATTTTGTGTGATTTGTGATTAGGTTTTGGAATTGGCTGGAAATGCTGCAAGGGACAACAAGAAGACTAGAATTGTGCCAAGACATATTCAATTGGCTGTTAGGAATGATGAAGAATTGAGCAAGCTTCTCGGTGATGTTACCATTGCTAACGGTGGTGTTATGCCCAATATTCACAACCTGTTNCTCCAAGAGGCTGGTACCTCCAAGGGTNCTGCTGCTGATGAANATNATNATTCTGACGACACGCAAACGGG
TARGET=80,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9569
PRIMER_LEFT_PENALTY=0.939039
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=GGATTCACCGATTTTGTGGTTA
PRIMER_RIGHT_SEQUENCE=TAGCAATGGTAACATCACCGAG
PRIMER_LEFT=51,22
PRIMER_RIGHT=258,22
PRIMER_LEFT_TM=60.939
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.9832
PRIMER_LEFT_1_PENALTY=0.939039
PRIMER_RIGHT_1_PENALTY=0.044122
PRIMER_LEFT_1_SEQUENCE=GGATTCACCGATTTTGTGGTTA
PRIMER_RIGHT_1_SEQUENCE=CTCTTGGAGNAACAGGTTGTGA
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=302,22
PRIMER_LEFT_1_TM=60.939
PRIMER_RIGHT_1_TM=60.044
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=1.0732
PRIMER_LEFT_2_PENALTY=1.055315
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=TAAGGATTCACCGATTTTGTGG
PRIMER_RIGHT_2_SEQUENCE=TAGCAATGGTAACATCACCGAG
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=258,22
PRIMER_LEFT_2_TM=61.055
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=1.0994
PRIMER_LEFT_3_PENALTY=1.055315
PRIMER_RIGHT_3_PENALTY=0.044122
PRIMER_LEFT_3_SEQUENCE=TAAGGATTCACCGATTTTGTGG
PRIMER_RIGHT_3_SEQUENCE=CTCTTGGAGNAACAGGTTGTGA
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=302,22
PRIMER_LEFT_3_TM=61.055
PRIMER_RIGHT_3_TM=60.044
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=1.1722
PRIMER_LEFT_4_PENALTY=0.939039
PRIMER_RIGHT_4_PENALTY=0.233127
PRIMER_LEFT_4_SEQUENCE=GGATTCACCGATTTTGTGGTTA
PRIMER_RIGHT_4_SEQUENCE=NAACAGGTTGTGAATATTGGGC
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=293,22
PRIMER_LEFT_4_TM=60.939
PRIMER_RIGHT_4_TM=60.233
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_00247 Ps_contig_mira-and-tgicl-ass_franssen_2859
SEQUENCE=GGCCTTTTCAGAATCTTATTCCCAAAATTCGTCTATTCGTACAATTTCGCAGGCTTAACGCCCAAACCCTATTCCCCCAAATTCTCTGTCTTTCTCTTCACTTCACTTCATCTACAACAATGGGATCCTCCGCGATGGTTTTAGACCCAAAACCCGTCTCAGAACCGCCGCCTTCTCTTCTATCATCAAAATCCGATTACCTTTACGGTGGCGACTCAGCCTCCGATGAAGATGACCTCTACAGCCGCCTCAAATCTCTGCAGCGACAACTAGAGTTCATCGACATCCAAGAGGAGTATGTAAAAGATGAACAGAAGAATCTAAAGCGCGAGCTTCTTAGGGCTCAGGAAGAAGTTAAGAGGATTCAATCTGTTCCACTCGTTATCGGTCAGTTCATGGAGATGGTTGATCAGAACAACGGTATTGTTGGATCAACTACTGGATCCAACTACTATGTCAGGATTCTCAGTACTATCAACCGTGAGCTTCTCAAGCCTTCTGCTTCGGTTGCTCTTCACCGACATTCCAATGCACTTGTTGATGTTCTTCCGCCGGAGGCTGATTCTAGTATCTCGCTACTTAGCCAGTCTGAGAAGCCTGATGTTTCTTACAATGATATTGGAGGATGTGATATCCAAAAGCAGGAAATTCGCGAGGCTGTGGAGTTACCTCTCACACATCACGATTTATACAAGCAAATTGGTATTGATCCTCCTCGAGGTGTTTTACTATATGGCCCCCCTGGAACCGGTAAAACAATGCTTGCCAAAGCTGTTGCTAATCATACCACTGCCGCCTTTATCAGGGTTGTGGGCTCTGAGTTTGTGCAGAAGTATCTTGGCGAGGGTCCAAGAATGGTTCGTGATGTATTTCGTCTCGCAAAAGAAAATGCCCCTGCAATTATATTTATTGATGAGGTTGATGCAATAGCTACTGCTAGGTTTGATGCCCAAACTGGAGCTGATAGAGAAGTACAACGTATCCTCATGGAACTTTTGAATCAGATGGATGGTTTTGACCAGACAGTGAATGTCAAAGTCATAATGGCAACCAATCGAGCAGACACTTTGGATCCCGCCCTCTTGCGTCCTGGAAGACTTGATCGGAAAATCGAGTTCCCCTTGCCCGATAGACGCCAAAAGAGGCTTGTATTTCAGGTTTGCACAGCAAAAATGAATTTGAGTGATGAGGTGGACCTGGAGGATTATGTTTCCCGTCCTGACAAAATTAGTGCTGCCGAGATATCTGCTATTTGTCAAGAAGCGGGGATGCATGCTGTTCGTAAGAATAGATATGTCATACTGCCAAAGGACTTCGAGAAGGGTTATAGGACCAACGTGAAGAAACCTGATACCGACTTTGAATTTTACAAGTGATGATGGAGGGTATTTTTCTGTATTTTGTTTGTGTATGTATGTGCTACGTTTCAGAATATAGTTGAAGGTTCAGAATAKAGTTAAGGTNAG
TARGET=96,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00248 Ps_contig_mira-and-tgicl-ass_franssen_2874
SEQUENCE=CAAAATATTCATTCCAAAAGTGTTGTCAAATTTTTTAGTAATTCCACCCTCTTTAWTTGAATTTGTTAAGTGGTACCACACGTCTTTGAGAACTTGGTCGTGTTGCATGAGAAAGAAAGAAATCATGGAGGCACCATCTTCATCTTTATTATTATTTTCGACAAAGTATTTAGCTAAAGGGTGAACACCATACAATCTTTCTTCATTACAATYTCCATCAACTTGTTCAATGGAACATGTCAGAATTTTGTAGCTAGMCAAAAGACGCAACAATCTATCCAACATAGAAGCTGCGTCTGGATTT
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00249 Ps_contig_mira-and-tgicl-ass_franssen_2895
SEQUENCE=TTACATCTGATTAGGTGAATCATTCTCTTTCTCTTTCTCTTTCTCTTCTTCRWACATTCAACACCCAAACACCTCTCTCTCTCTCTCTCCGCATATAACTCCTCCTGCTATCATCCACATAGACACAACAACCACCTGTGAAGATGGGTAGGAGCTTAAGTTAAGGCTGCACGTCGGGAGACGCAGTTATTGTACCTTTCAGTTGGGTTTGGGTTTTTTTCTTCCTTCCAGTTGGG
TARGET=73,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00250 Ps_contig_mira-and-tgicl-ass_franssen_2896
SEQUENCE=CGGGTTTTTAACTTTTTTTACTTTTTTAAACTTTTTCTTAACTAAWTGAACAACAACATCAACAACAACACAAATCCATTTTGAATTCTCTCTCTCTCTCTCTATTGAATGGAGTCTTTCTCTTACACTAACACCAACCCTTCTCATCTTCNCTTGTCACTTCTCTAACAAGCTATGTGTAGTTTCATCAAAGGAAAGGTAAAGGTTTCTTTTC
TARGET=87,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00251 Ps_contig_mira-and-tgicl-ass_franssen_2899
SEQUENCE=TCGGGGGAACACAAACTCAGSATGAACAACAACATCAACAACAACACAAATCCATTTTGAATTCTCTCTCTCTCTCTCTATTGAATGGAGTCTTTCTCTACACTAACACCAAACCTTCTTCATCTTCTACTTGTCACTTCTCTAACAAGCTATGTGAGTTTCATACAAAGGAAAGTAAAGGTTTCTTTTCTTGAT
TARGET=63,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00252 Ps_contig_mira-and-tgicl-ass_franssen_2904
SEQUENCE=GCTCTGAAGTATTTTAGGGTTTTTGTTTCCGTGAACAGTTTCTTCTTAGGTCTGAGCCAATATCCTCTGACAAAAATGGTTCTTACAAAAACGATTATTGTATTTTACGTTAAACCCCTCCTGCTGAGCTTAGAAGAAGAAGAAGCAACAAGCTCAAGTCGTTCTTGTTCACGTACCAACCAACTTCTTCT
TARGET=132,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2278
PRIMER_LEFT_PENALTY=0.163658
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=TCTGAGCCAATATCCTCTGACA
PRIMER_RIGHT_SEQUENCE=GTTGGTTGGTACGTGAACAAGA
PRIMER_LEFT=50,22
PRIMER_RIGHT=183,22
PRIMER_LEFT_TM=59.836
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.2376
PRIMER_LEFT_1_PENALTY=0.163658
PRIMER_RIGHT_1_PENALTY=0.073989
PRIMER_LEFT_1_SEQUENCE=TCTGAGCCAATATCCTCTGACA
PRIMER_RIGHT_1_SEQUENCE=TTGGTACGTGAACAAGAACGAC
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=178,22
PRIMER_LEFT_1_TM=59.836
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.3292
PRIMER_LEFT_2_PENALTY=0.265053
PRIMER_RIGHT_2_PENALTY=0.064105
PRIMER_LEFT_2_SEQUENCE=AGGTCTGAGCCAATATCCTCTG
PRIMER_RIGHT_2_SEQUENCE=GTTGGTTGGTACGTGAACAAGA
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=183,22
PRIMER_LEFT_2_TM=59.735
PRIMER_RIGHT_2_TM=59.936
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=0.3390
PRIMER_LEFT_3_PENALTY=0.265053
PRIMER_RIGHT_3_PENALTY=0.073989
PRIMER_LEFT_3_SEQUENCE=AGGTCTGAGCCAATATCCTCTG
PRIMER_RIGHT_3_SEQUENCE=TTGGTACGTGAACAAGAACGAC
PRIMER_LEFT_3=47,22
PRIMER_RIGHT_3=178,22
PRIMER_LEFT_3_TM=59.735
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=0.3565
PRIMER_LEFT_4_PENALTY=0.292414
PRIMER_RIGHT_4_PENALTY=0.064105
PRIMER_LEFT_4_SEQUENCE=TGAGCCAATATCCTCTGACAAA
PRIMER_RIGHT_4_SEQUENCE=GTTGGTTGGTACGTGAACAAGA
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=183,22
PRIMER_LEFT_4_TM=59.708
PRIMER_RIGHT_4_TM=59.936
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_00253 Ps_contig_mira-and-tgicl-ass_franssen_2924
SEQUENCE=CRTTCCAAATACTCAATAAATTAAAATCACGTAAATAACTTAAACAAACCTATTTGATGTCTCTGATGTCTCTGCAACTTAATAAAACCAACATGTAACTGAGCACAAGGTCCAGACAAACAAACAAAATTTGGTAACAAGAAAATTAAACAAAACATAAATCATAGCAACAATGTCTAAACGCAAGAAATTAAAAAATTGTTCACTGATAATATCTCTTCTGACAAGACAAAAAACAGAAACTACTTTATCCGTCTTSGWTASRSAACTGTTTTTCC
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00254 Ps_contig_mira-and-tgicl-ass_franssen_2935
SEQUENCE=GAAGCCAAGTAAGGCKTCKGRGGACCTAAGAGTGCAAAGCAAACAAGAGCAGACTTAGAAGCTACTTCGAATTAGGTAAAATATAATTTGACATGGGAAATAGTCTCAACGGTGTTAGTCTCTTCAACGACGACACAGATTATCCATGTCGGATACAGCTAGGTAGATATGCATTCATCGATGTGACACAAACAGAGAACAAAGGCGATTGCAATGATAGTGATGTGAGGCAAACAAAGAACAAAGGCGATTGCAGTGATAATGATGATGAAATTTCCAGTGATAATGATGAAGCTCCCAGTGATAGTGATGATGAAGTTCTCAGTGATGATGATGAAGTCCCCGGTGATAATGATGATGAAATTCTCAGTGATAGTGATGATGAAGTTACCAAAGTGACCAAGATGTTATACACCGTAGGTAGGTCGTCCATATGTGAACTCAAATTTAAGAGAAGACATAAAGAGGCATCATTATCTCTCCTTGATGTCGAGAATGGTTCCAATTCCAATTGTGTGGATGTTAAAATTATAATAAGAAAAACTGATAAGGGATATCTCGAGATACCTTATCCTGATTTCTCCTTTAGAGGCGGAGGGATATACATTTTCATGAGGGATAATAATTCTAACAGAACCATCAATGCATGGGGAGGTGTGACAGAATTAACAACCTTTCTCTATGGTGAAGGAAGGAGGGTAAGTCTTGTTGTTATAGAGTCTAAGAAAAAGAGTATAAGTGATCCAAATCCCTATATGGTGACTGTGGTTCACTATTTTGCTATTGATGATGGAAATTTTTTCAATGCAAAAATGGATATTGGCCTTTCTGTGATGGTAAAGATTCAACCATCAGATGATTTGGGATTGAAATATACTGTAGAGGGTCCAGAGAGACACCCTAGTTCATCATTATTTTACATGTTTGAACGAGTAAAAAGACTTAATGGGGCATGGAAGTCTAATTTGTGTCCTCATTGTGCAAATCTTCAGATGCGGCGTAAATCCTGGCAGACCTCTGAAAGTGAAGACAGCGATTATGTTCCACGACGACATGGTAATGGTAAGCAGAAGTCAGAAAGTATGGTTGCCAATGATTCTAACGTTAAGGGAAATTATAATGGTAGTTTGTTTGGGAAGTATTTCCATTTTCATACAAGGTGAAATATGTAATGGAATCCTTTAATATGAAAAGAAAGAAAATAAAAAGGTTAGGGCAAGGTTAGCCACGGCTAAAGATGGATACCATACCTAACTAAGTATGTTGTTAATTAGAGAGCATTTTAAAATATTGGTAAGTAATTTTGTGTGTTGCACACACCTAGTTGAATGTAAAGAGAGAGGGTTAGCTATCTTCCATGAATGTAATATTTGACACTTAAGATTTGATCATACCTTTAATGTCAAATTACTTGAAATAAATAAAAAAAATACTTTTGTTCAACC
TARGET=326,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00255 Ps_contig_mira-and-tgicl-ass_franssen_2959
SEQUENCE=GTCTAGAAAGTAGGCGTTCACTGGAGTTCAACTACTTTTGTTGTTCCACCTTTCCTTGCTCTCAACCTTGAGTGCCTGCAGAGCTTTCCTTTTCTCTTCTAAAACTTTCTCATATTCTTCTAGTGTCATTTCCTTGTCCTCAGGCTCCTTTTCTTCAGCTTCATTAGCAGGTGCTTCTTTGTTTCCTTCAGCAGCTGCATCTTCTTCACCAGCAGGCTTCTCCTCAGACACATTCTTTTCGGTTTCGTTAGCAACTTCATCAGTCACCTGAGCAATTTCATCTGACTGAGTTCCCCAATTACCACGTCCAGCACCTTCACGTTTGAATCCGCCTCCTCGTCCAGTCCCGCTGTGGCGATCAAATGTCCTTCGTGGACGTCCTTCTTCACCACCTTCACCATTGCTAAATCCTCCACGACGACCGCCACCTCCGCCGACACGGTAAGGAGCACGAGGTGCACCATAACCACGCCTTTCAGAGGGATTCCCAGAATCTCCTTCAAAAGAACCTTGATTAGCAGGAGGAACAGGCAGTGAAGAGGAGTTATCGTCATTGGAAAAGTCACGACCAAAGCCGCCGCCGCCGCCACCACCACCGCGACCACGTCCAAATCCTCCTCCATATCCCCGTCCGCCTCCACGGCCACCACGAGATGGTTCGTTTCTTGACTCCCTCACAGCCTGAGAGGGAGGTAGTGGTTTGGAAGGCAAAAGAGCAGCCTTTTTGGGAGCAACTTGTTTGCCTTGTTCCAAACCCTTTTTGGGAGCAGCCG
TARGET=574,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0386
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=TATCGTCATTGGAAAAGTCACG
PRIMER_RIGHT_SEQUENCE=CTTTTGCCTTCCAAACCACTAC
PRIMER_LEFT=545,22
PRIMER_RIGHT=713,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=5.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=0.1124
PRIMER_LEFT_1_PENALTY=0.077340
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=GAACAGGCAGTGAAGAGGAGTT
PRIMER_RIGHT_1_SEQUENCE=CTTTTGCCTTCCAAACCACTAC
PRIMER_LEFT_1=524,22
PRIMER_RIGHT_1=713,22
PRIMER_LEFT_1_TM=59.923
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.1280
PRIMER_LEFT_2_PENALTY=0.093026
PRIMER_RIGHT_2_PENALTY=0.035021
PRIMER_LEFT_2_SEQUENCE=AGGCAGTGAAGAGGAGTTATCG
PRIMER_RIGHT_2_SEQUENCE=CTTTTGCCTTCCAAACCACTAC
PRIMER_LEFT_2=528,22
PRIMER_RIGHT_2=713,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=60.035
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=0.1567
PRIMER_LEFT_3_PENALTY=0.003589
PRIMER_RIGHT_3_PENALTY=0.153161
PRIMER_LEFT_3_SEQUENCE=TATCGTCATTGGAAAAGTCACG
PRIMER_RIGHT_3_SEQUENCE=GTGAGGGAGTCAAGAAACGAAC
PRIMER_LEFT_3=545,22
PRIMER_RIGHT_3=677,22
PRIMER_LEFT_3_TM=59.996
PRIMER_RIGHT_3_TM=60.153
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=0.1633
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.159665
PRIMER_LEFT_4_SEQUENCE=TATCGTCATTGGAAAAGTCACG
PRIMER_RIGHT_4_SEQUENCE=CAAACCACTACCTCCCTCTCAG
PRIMER_LEFT_4=545,22
PRIMER_RIGHT_4=702,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=60.160
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_00256 Ps_contig_mira-and-tgicl-ass_franssen_2981
SEQUENCE=GGATTTTTGTTTGTTTGTTTCTGAATCGGAACTTTACTTTACTTGTATTTTACATAAAGCATGTTCTGTCCAAGATCACTGCTCCTCCCCAAAAGCTCCTTTACCCTCTCTTTCTTCTCCCATCGCTTCACCTGTAACTACAAGATTGTTGAAATGGCGCGATCTTCGTTGGATGAGATATCAGACTCGGGAGCATTCACAAGATCTGCTTCCACATTCCGACAGTTCGTTTCAAGGGATCTTAATTCCCTAATTCCACCTGAGTCTGGAAGTNTCATCTNTACATNTNAT
TARGET=6,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00257 Ps_contig_mira-and-tgicl-ass_franssen_2986
SEQUENCE=CTTTAGCAATTGAGACTRCTTATGAGGRCCTTGAACGATCCGGCTTCTCTGATGGTAAATCTATACTTGTTCTTGGCGGTGCCGGTGGAGTTGGAACCCATATCATTCAGCTTGCCAAACATGTTTTTGGTGCTTCCAGAGTCGCAGCTACATCTAGCACCAAGAAACTAGAATTGTTAAGAGAGTTAGGAGCTGATTTGCCTATTGATTATACAAAGGAGAATTTTGAAGAGTTGGCTGAGAAGTTTGATGTGGTGTTTGATACAGTCGGGGAAACTGAGAAGGCATTCAAGGCTGTGAAAGAAGGTGGCAAAGTTGTGACAATTGTGCCGCCAGGAATTCCACCAGCAATAGTGTTCATACTTACTTCAAATGGGGCTATCTTAGAGAAACTAAAGCCTTATTTGGAGAGTGGAAAGGTGAAGGCAGTGCTTGATCCCAAAAGTCCATTTCCATTTTCTCAAGCTGTTGAAGCATTTTCCTATCTCGAAACTGGCAGAGTTACTGGAAAACTAGTTATCCATCCCATACCATGAAATTCAAAACAACCCAATACAATCTTTGGAATCTGAATGTGTGTGTGTTTGTATTTCTGTATGATCTCTACTTTCAGTGTAGTCATCATCTCTTACTACTTACTAGTGTGTTTGTATGATGTGAGAACTTGAAAATGGGTTAGTCTTCCTTTGGATTTGTATTTGGTTTGGGGTTGAAAAAACACTACGTTTGGCTTTTGTAGTTTTAGTTAATGGAATAATAATGTGATAACAAGTTTGCTTCATATATTACTATATTGTTCATCTTGGGAGCAACATTTCTTAAAAATCTCAAATGTTTATGGTTGTGTT
TARGET=574,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00258 Ps_contig_mira-and-tgicl-ass_franssen_3012
SEQUENCE=GGSCTCACATCACAATACACAAATACACGAATACACATACACTCTCTCTCTCTCTCCCTCTTTCTCAATCCGCGTTATTACCCTAAAAACAACAAATTCTTCAATCGAATCCAACAATGGCTCTGCGAGTAACAACCCCTTTCCCTACTCAAACTTCCTCCTTCTCCCTCCCTCAAATGGCCAGTCTCAGATCCTACCCAAATTCGTCATGGCCTTCCCACCCTCCGCTCCGGTTACCAAAAGGAGGGTTTAG
TARGET=42,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00259 Ps_contig_mira-and-tgicl-ass_franssen_3019
SEQUENCE=CTTTTTTTTTTSGKAATATTAATAAACGAATATATCATTTAAAAATAAAAGTCAAGCAAAATATATGTGTGTAGGATATGCATGTGTAGTTACTTGTCCTTTGTGAGACAAATGTACATGCTATAGGAATATGACAGTAATACGATACAAAATATCTTTTTCAGTCGTAATCTTAGTTATAAAATGGCATGGCATGGCAGCCTTACAAAATATATCTTCACGACCAAACAAAATCCCATATCCATCAA
TARGET=184,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00260 Ps_contig_mira-and-tgicl-ass_franssen_3023
SEQUENCE=GCTATTTCCCTTGGGGAGAATGATATCAAGTTAAGTTATTTCCTTCCCTTGACACCTGAATCTTCAAGCTCTGATTGGTAGCTGATAATTCGTGGCTTATAGGGTCTGATATGGTTGTTGTTGTTGTTAATAAATGAGTTGTTCATCAGMCCAAATCTACGAGGCTTAGGGCAAACCACAGATTCCATCCTCATCTCTTACACAGCTTCTCATGGCGTTCTTCTGATGGTAACCACAGTGATTCATTCTTTCTCTCTAATTTCACACCTAGAAAACCAAAGATACTTAAACAAAGAACACCTGGAAGGTAAGCTAGAATCAGAGACCTCCTACGTAAAGGAAGGGAAGGAAAAGGAAAGGGAAAAAAATACG
TARGET=114,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00261 Ps_contig_mira-and-tgicl-ass_franssen_3025
SEQUENCE=GGTTTTTCCGTATTTTTTTTTCCCCCCTTTTTTCCCTTTCCCTCCTTAGTAGGAGGCTCTGATTCTAGCTTACCTTCCAGGTGTGAAAATTAGAGAGAAAGAATGAATCRCTGTGGTTACCATCAGAAGAACGCCATGAGAAGCTGTGAAGAGATGAGGATGGAATCTGTGGTTTGCCCTAAGCCTCGTAGATTTGGTCTGATGAACAACTCATTTATTAACAACAACAACAACCATATCACGACCCTANAAGCCACAAATTATCAGCCTACCAATCAGAGTCTTAGAAGTATTCGAGGTGTACGAAGGGAANTTCCTTTCGTTTAGTAA
TARGET=220,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00262 Ps_contig_mira-and-tgicl-ass_franssen_3035
SEQUENCE=GTCGGGACCTACAAAAACAAACTCACGCTCTCTCTCTCAARGGGGATTCATTCCGCCTCAAACCCTAGCCGCCGCTAAACCCTTTTCCTCTTCTCTTCAACAACCGTCAAAACCCTAAATCTCTCTCTTTCAAACCGCGAGCTCGCCGCCATCACCACCGGCGAAAACCCCAACGAACACCATACAACCTCCACGCCGTAACATCTAAACCATCAATCGTCTTCCCCATTTCATCCGCCGAAATACACCGATTAACCTCCGCCTTGACCAAAAACCCCCAAATCCTCACTTCATTTCGTCCCTTTCGAAATTA
TARGET=28,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00263 Ps_contig_mira-and-tgicl-ass_franssen_3041
SEQUENCE=GAAGCAGTGGTAGTCAACGTCGGGGGCTAATTTCCATTTTGTTAGTTCGGTTTAGGTGGAGAGAGAGAGAGAGTGTGTTGAGATTTTTTCTTTCATTTTTCTTATCAATTTCTCTTTATTTGTTAGGTTGTTTTTTCTCTTTCCTCATTTTTGTAGGTATATTGTGGAAGAAGACTTTGTAGCTTGACTTTGTTCAAAAGCATACGATTCAGGGAAGGCCGTAAAGTTGGAGTGAAATTAGATTGAAATCTTAGAAGTTTCTCTCTTTGGATTTGAAGAAATGGAGGTAYATGGAAATTCTATGGTTGCCGCTCCTTCAAATGTTATTTATCTGTCAAGTAT
TARGET=59,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00264 Ps_contig_mira-and-tgicl-ass_franssen_3043
SEQUENCE=TCACACAAAAACCCTACAACAAGTTTAAGACAAAAAAAAGTAGATTTTTTTGACCAAAAAAATAAGAGGAGAATCYTCGTTTTTTATAGAAGCCGGAAAATTAAAAAAATWAAATTAAAAATGTAGAATTTACATAAGGAGAAATGTGATCAAGCGTTTTTATGAGTGCCCCAGTTTGAGAAAAACCGGATAGGATCAAAATCGGAGTCAATTAAAATATATATTCTGAAAATTGTTTTATAAAATATACATTGAACCAGAAATGGAAACAAAGGGGATTAGGGTTTTTAGATGGATCATCAGAGAACAAACAACAAACGGAGAGAGAGTGAGAGGGAGAGAGAGAAGAGTTGGTGTTGTTGGAATAACAAAGGCTTTGGCTTTCGCTTACACTCTCTTGGCCTTTCTTTGTGTTGTC
TARGET=337,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00265 Ps_contig_mira-and-tgicl-ass_franssen_3054
SEQUENCE=CCGACTCCTTAAGTAGTTTTCCGGTCTCAGAATCCCAAATACTCATAGAGACATTCAACTTCATTCACTGTCACAGTAAAGAGTATTTCTAATTCCGATCAACGAAGATAAACATCAAACACATCAAAACCATCCACATCTTAGCGCTTCTCTTTCCCTGATTTCGCTCCATCACTTCTCCCCTTTTTATTTATTTAATTTGGGGTTTTTTTCTGGAATTGAAAAAAAATCGATAATTGTSTATTGCAGNTCTAAGNTTGGATTTGAGAGGTTTNACTTNCGTCATGCAGTNCACGCAACAACGNAT
TARGET=186,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00266 Ps_contig_mira-and-tgicl-ass_franssen_3055
SEQUENCE=AGGACGTCCACGAAAATAAATACATGGTCAACAACAACAAAATTACCCCGAAACCAAGTGTCCCCTCCGTAAAGCACACACTCATAGAGACATTCAACTTCATTCACNGTCACAGAAAGAGATTCAATCCGATCAACGAAGATAAACATCAAACACATCAAAMCCATCCACATCTTAGCGCTTCTCTTTCCCTGATTTCGCTCCATCACTTCTCCCCTTTTTATTTATTTAATTTGGGTTTTTTC
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00267 Ps_contig_mira-and-tgicl-ass_franssen_3055
SEQUENCE=AGGACGTCCACGAAAATAAATACATGGTCAACAACAACAAAATTACCCCGAAACCAAGTGTCCCCTCCGTAAAGCACACACTCATAGAGACATTCAACTTCATTCACNGTCACAGAAAGAGATTCAATCCGATCAACGAAGATAAACATCAAACACATCAAAMCCATCCACATCTTAGCGCTTCTCTTTCCCTGATTTCGCTCCATCACTTCTCCCCTTTTTATTTATTTAATTTGGGTTTTTTC
TARGET=220,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00268 Ps_contig_mira-and-tgicl-ass_franssen_3070
SEQUENCE=GAAGGCAGTGACAGATCGACTTTATCAGATTTTTTTGTTGTAACCCCTTATATTTATTTATCCTTCCGTAGATAGTCGGGGAATGAGAGAGAGAGAAGAACAAGGGTGACTCTCATTCCCCAAACCCTATTCCTCTCTTTCCGTTTCCACGCTTTCTCTTCTCTGACTTTCATTTCAGGATTAACGCCGTCGTTTCGCCGCCGATCATCGTCTGATTAAGAGCAGTGAAGAATAAACTGATGGCTAACATGGATGGCAAATCACTCCTGAGTCCTAAGAATTCCATTCCCATACCCCCTCCTTCGCCTCAATCAGCAGCAACAGGTTATCCTTGCGAGTATGTGGTGATGAATAATTTAGCACCCAGACGGCGAAGCAGTTGTGTCCCTACTGCAACAAGAGATTTGTGGGAGCGTCTTTTCAATGAAGGTTATAAAGCCAATGTTCGCA
TARGET=85,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5499
PRIMER_LEFT_PENALTY=0.519744
PRIMER_RIGHT_PENALTY=0.030114
PRIMER_LEFT_SEQUENCE=TATCCTTCCGTAGATAGTCGGG
PRIMER_RIGHT_SEQUENCE=GGCGTTAATCCTGAAATGAAAG
PRIMER_LEFT=58,22
PRIMER_RIGHT=187,22
PRIMER_LEFT_TM=59.480
PRIMER_RIGHT_TM=59.970
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=0.6780
PRIMER_LEFT_1_PENALTY=0.519744
PRIMER_RIGHT_1_PENALTY=0.158297
PRIMER_LEFT_1_SEQUENCE=TATCCTTCCGTAGATAGTCGGG
PRIMER_RIGHT_1_SEQUENCE=AGGATAACCTGTTGCTGCTGAT
PRIMER_LEFT_1=58,22
PRIMER_RIGHT_1=331,22
PRIMER_LEFT_1_TM=59.480
PRIMER_RIGHT_1_TM=60.158
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=274
PRIMER_PAIR_PENALTY_2=0.6906
PRIMER_LEFT_2_PENALTY=0.519744
PRIMER_RIGHT_2_PENALTY=0.170825
PRIMER_LEFT_2_SEQUENCE=TATCCTTCCGTAGATAGTCGGG
PRIMER_RIGHT_2_SEQUENCE=GAGTGATTTGCCATCCATGTTA
PRIMER_LEFT_2=58,22
PRIMER_RIGHT_2=265,22
PRIMER_LEFT_2_TM=59.480
PRIMER_RIGHT_2_TM=59.829
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.6930
PRIMER_LEFT_3_PENALTY=0.675181
PRIMER_RIGHT_3_PENALTY=0.017846
PRIMER_LEFT_3_SEQUENCE=CTTCCGTAGATAGTCGGGGAAT
PRIMER_RIGHT_3_SEQUENCE=CACCACATACTCGCAAGGATAA
PRIMER_LEFT_3=62,22
PRIMER_RIGHT_3=346,22
PRIMER_LEFT_3_TM=60.675
PRIMER_RIGHT_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.7053
PRIMER_LEFT_4_PENALTY=0.675181
PRIMER_RIGHT_4_PENALTY=0.030114
PRIMER_LEFT_4_SEQUENCE=CTTCCGTAGATAGTCGGGGAAT
PRIMER_RIGHT_4_SEQUENCE=GGCGTTAATCCTGAAATGAAAG
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=187,22
PRIMER_LEFT_4_TM=60.675
PRIMER_RIGHT_4_TM=59.970
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_00269 Ps_contig_mira-and-tgicl-ass_franssen_3079
SEQUENCE=GTTCGGGGGGKCAGAGATTGGTTTGGTTAACGAACGCTTCAACGGCAAAATTTCCCGGGCTACAAGGCTTCCTGTCTCTCTCTCTCTTTCATCTCCATGCGCGACAATTCCCATTTTCTCACTCATAATTTCCATCTCCTCCAAAAGGATGCGTAATTGGAAGGGGGATTGGCAATAACTGGAGGAGGAGAAGGTAGGGAGGTGTGGTGGGTGAGAGTGAGTTGGGTCTAAGGTGAAGNT
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00270 Ps_contig_mira-and-tgicl-ass_franssen_3082
SEQUENCE=AATAGTTTGAGTCGAACCACCGGTAATAGCTTTCTTTTTTAAGTTTCTCCCAGTTTAGCTTCCACCTTCTCTTTAACAGGGGCGGGAACTTTCYTCTCCTAGCTCTTTCAATTGCTTCTCAGTCTGGTAGACAACAGAGTCTGCCTGGTTCTTGGTGTCGATTGCTTCCCTCTTTTCTTTGTCCTCTTTTGAAAATCTCTCTGCCTCGCTCACCATTCTCGACAACATCATCTTTTGCCCATATAAACAAAACAAACAAACAAAAAAACCAA
TARGET=250,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00271 Ps_contig_mira-and-tgicl-ass_franssen_3095
SEQUENCE=CGTCGGGGACAAACATCATTTTTTCCACTATTCTTGTGTTCTCTTCTCTTCTCTGATCTTCTTCTCTCCCACACCACAACTCACAAACATCATTTTTTCCACTATTCTTGTGTTCTCTTCTCTTCTCTGATCTTCTTCTCTCCCACAGTTAGCTCAGAAGAACTCATCCAAAATGGTTATCTCATCAACTGTCAAACATCCAGATGAACATAGTGAAAGCATAGACTGTACTTTTGCTTCTAGATACGTGCGTGAAGCAGTTCCAAAGTTCAAGCTGTCAGAGAGTTCTATACCAAAAGATGCAGCATATCAGATAATAAACGATGAGTTGATGTTAGATGGGAAACCAAGGCTTAACTTGGCTTCATTTGTCACTACTTGGATGGAACCTGAGTGTGATAAACTCATCATGGATTCGCTTAACAAGAACTATGTTGACATGGATGAGTATCCTGTCACCACTGAGCTTCAGAATCGGTGTGTGAATATAATAGCACATCTATTCAATGCTCCTATTGGTAAAGAGGAAACAGCAGTAGGTGTTGGAACCGTAGGATCATCAGAAGCAATAATGTTAGCTGGTTTAGCTTTTAAAAGGAAGTGGCAAACTAAGAGAAAATCAGAAGGCAAACCTTACGATAAGCCCAACATCGTTACCGGGGCTAATGTGCAGGTTTGTTGGGAAAAATTTGCTAAGTACTTTGAGGTTGAACTAAAGGAAGTGAAACTCTCGGAAGGATACTATGTGATGGATCCATTGAAAGCAGTTGAAATGGTAGATGAGAACACTATCTGTGTTGCAGCCATTTTGGGTTCAACGCTGACCGGAGAGTTTGAGGATGTGAAGCTTCTTAACGAACTTCTTACTGTAAAGAACAAAGAGACGGGATGGGATACGCCGATTCATGTAGATGCTGCGAGCGGTGGTTTTGTTGCTCCGTTTCTCTACCCTGATCTGCTATGGGATTTTCGCTTGCCATTGGTGAAAAGTATCAATGTTAGCGGTCACAAGTACGGTCTTGTTTATCCCGGTATTGGTTGGGTTGTCTGGAGGAATCAAGATGACTTGCCGGATGATCTTGTTTTTCATATCAATTATCTTGGATCTGATCAGCCAACTTTCACATTGAATTTCTCCAAAGGATCGAGTCAAATCATTGCTCAATACTACCAATTCATACGACTTGGATTCGAGGGCTACAAAAGTGTGATGGAAAATTGCGTTGGACGAAACACGGAAGTAATTCGTAAGGAAAAAGGAAAGGGTAATTTAGGAAAGTAAAACGTTAGGGTTAAAAGTTTTAAGTATTTGGTACGTACCGAAAGGGGTACCGGTTCCGGG
TARGET=39,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3776
PRIMER_LEFT_PENALTY=2.347318
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=GTCGGGGACAAACATCATTT
PRIMER_RIGHT_SEQUENCE=TCTGACAGCTTGAACTTTGGAA
PRIMER_LEFT=1,20
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=59.653
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=2.3783
PRIMER_LEFT_1_PENALTY=2.347318
PRIMER_RIGHT_1_PENALTY=0.030936
PRIMER_LEFT_1_SEQUENCE=GTCGGGGACAAACATCATTT
PRIMER_RIGHT_1_SEQUENCE=AACTGCTTCACGCACGTATCTA
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=261,22
PRIMER_LEFT_1_TM=59.653
PRIMER_RIGHT_1_TM=59.969
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=2.6910
PRIMER_LEFT_2_PENALTY=2.347318
PRIMER_RIGHT_2_PENALTY=0.343680
PRIMER_LEFT_2_SEQUENCE=GTCGGGGACAAACATCATTT
PRIMER_RIGHT_2_SEQUENCE=TCTCTGACAGCTTGAACTTTGG
PRIMER_LEFT_2=1,20
PRIMER_RIGHT_2=283,22
PRIMER_LEFT_2_TM=59.653
PRIMER_RIGHT_2_TM=59.656
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=2.6910
PRIMER_LEFT_3_PENALTY=2.347318
PRIMER_RIGHT_3_PENALTY=0.343680
PRIMER_LEFT_3_SEQUENCE=GTCGGGGACAAACATCATTT
PRIMER_RIGHT_3_SEQUENCE=CTCTGACAGCTTGAACTTTGGA
PRIMER_LEFT_3=1,20
PRIMER_RIGHT_3=282,22
PRIMER_LEFT_3_TM=59.653
PRIMER_RIGHT_3_TM=59.656
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=2.7599
PRIMER_LEFT_4_PENALTY=2.347318
PRIMER_RIGHT_4_PENALTY=0.412596
PRIMER_LEFT_4_SEQUENCE=GTCGGGGACAAACATCATTT
PRIMER_RIGHT_4_SEQUENCE=GCTTGAACTTTGGAACTGCTTC
PRIMER_LEFT_4=1,20
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=60.413
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_00272 Ps_contig_mira-and-tgicl-ass_franssen_3095
SEQUENCE=CGTCGGGGACAAACATCATTTTTTCCACTATTCTTGTGTTCTCTTCTCTTCTCTGATCTTCTTCTCTCCCACACCACAACTCACAAACATCATTTTTTCCACTATTCTTGTGTTCTCTTCTCTTCTCTGATCTTCTTCTCTCCCACAGTTAGCTCAGAAGAACTCATCCAAAATGGTTATCTCATCAACTGTCAAACATCCAGATGAACATAGTGAAAGCATAGACTGTACTTTTGCTTCTAGATACGTGCGTGAAGCAGTTCCAAAGTTCAAGCTGTCAGAGAGTTCTATACCAAAAGATGCAGCATATCAGATAATAAACGATGAGTTGATGTTAGATGGGAAACCAAGGCTTAACTTGGCTTCATTTGTCACTACTTGGATGGAACCTGAGTGTGATAAACTCATCATGGATTCGCTTAACAAGAACTATGTTGACATGGATGAGTATCCTGTCACCACTGAGCTTCAGAATCGGTGTGTGAATATAATAGCACATCTATTCAATGCTCCTATTGGTAAAGAGGAAACAGCAGTAGGTGTTGGAACCGTAGGATCATCAGAAGCAATAATGTTAGCTGGTTTAGCTTTTAAAAGGAAGTGGCAAACTAAGAGAAAATCAGAAGGCAAACCTTACGATAAGCCCAACATCGTTACCGGGGCTAATGTGCAGGTTTGTTGGGAAAAATTTGCTAAGTACTTTGAGGTTGAACTAAAGGAAGTGAAACTCTCGGAAGGATACTATGTGATGGATCCATTGAAAGCAGTTGAAATGGTAGATGAGAACACTATCTGTGTTGCAGCCATTTTGGGTTCAACGCTGACCGGAGAGTTTGAGGATGTGAAGCTTCTTAACGAACTTCTTACTGTAAAGAACAAAGAGACGGGATGGGATACGCCGATTCATGTAGATGCTGCGAGCGGTGGTTTTGTTGCTCCGTTTCTCTACCCTGATCTGCTATGGGATTTTCGCTTGCCATTGGTGAAAAGTATCAATGTTAGCGGTCACAAGTACGGTCTTGTTTATCCCGGTATTGGTTGGGTTGTCTGGAGGAATCAAGATGACTTGCCGGATGATCTTGTTTTTCATATCAATTATCTTGGATCTGATCAGCCAACTTTCACATTGAATTTCTCCAAAGGATCGAGTCAAATCATTGCTCAATACTACCAATTCATACGACTTGGATTCGAGGGCTACAAAAGTGTGATGGAAAATTGCGTTGGACGAAACACGGAAGTAATTCGTAAGGAAAAAGGAAAGGGTAATTTAGGAAAGTAAAACGTTAGGGTTAAAAGTTTTAAGTATTTGGTACGTACCGAAAGGGGTACCGGTTCCGGG
TARGET=113,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4258
PRIMER_LEFT_PENALTY=0.395499
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=ATCTTCTTCTCTCCCACACCAC
PRIMER_RIGHT_SEQUENCE=TCTGACAGCTTGAACTTTGGAA
PRIMER_LEFT=55,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=59.605
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.4264
PRIMER_LEFT_1_PENALTY=0.395499
PRIMER_RIGHT_1_PENALTY=0.030936
PRIMER_LEFT_1_SEQUENCE=ATCTTCTTCTCTCCCACACCAC
PRIMER_RIGHT_1_SEQUENCE=AACTGCTTCACGCACGTATCTA
PRIMER_LEFT_1=55,22
PRIMER_RIGHT_1=261,22
PRIMER_LEFT_1_TM=59.605
PRIMER_RIGHT_1_TM=59.969
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.6258
PRIMER_LEFT_2_PENALTY=0.395499
PRIMER_RIGHT_2_PENALTY=0.230329
PRIMER_LEFT_2_SEQUENCE=ATCTTCTTCTCTCCCACACCAC
PRIMER_RIGHT_2_SEQUENCE=TCTGATATGCTGCATCTTTTGG
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=313,22
PRIMER_LEFT_2_TM=59.605
PRIMER_RIGHT_2_TM=60.230
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.6586
PRIMER_LEFT_3_PENALTY=0.628294
PRIMER_RIGHT_3_PENALTY=0.030276
PRIMER_LEFT_3_SEQUENCE=GATCTTCTTCTCTCCCACACCA
PRIMER_RIGHT_3_SEQUENCE=TCTGACAGCTTGAACTTTGGAA
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=281,22
PRIMER_LEFT_3_TM=60.628
PRIMER_RIGHT_3_TM=60.030
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=0.6586
PRIMER_LEFT_4_PENALTY=0.628294
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=TGATCTTCTTCTCTCCCACACC
PRIMER_RIGHT_4_SEQUENCE=TCTGACAGCTTGAACTTTGGAA
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=281,22
PRIMER_LEFT_4_TM=60.628
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_00273 Ps_contig_mira-and-tgicl-ass_franssen_3096
SEQUENCE=TTTCGCTAAGGAAACAAGACAAGTCTTATTCATTCATTCATATAAATATATGGTTTAGAGATACATTGACAATGGTTATATCTCTACATAGATAAATAAAGTAACAATGAAACAATCCTTACACACATTAAATTTAATTGTACCGCACAAACAAAGTAGCTCACAAAACACAACAAACATTTGACAACACTAGCATGTTCCAACTCTCTTTCCTGTAGCAAGCTTCTTCCAATAACTTGCAATCTCAGTTTGAGTCTCTGTAATATCTTTCTTAACCTCCTCGCTTGTCTCACTCGCGATAACCGCGACATGAGCACTTTTCGAGGAGATGGTACTTGGAATTGTCTCCAAGAGAGCC
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3172
PRIMER_LEFT_PENALTY=1.302444
PRIMER_RIGHT_PENALTY=0.014720
PRIMER_LEFT_SEQUENCE=TTCGCTAAGGAAACAAGACAAG
PRIMER_RIGHT_SEQUENCE=CAAGCGAGGAGGTTAAGAAAGA
PRIMER_LEFT=1,22
PRIMER_RIGHT=286,22
PRIMER_LEFT_TM=58.698
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=1.3213
PRIMER_LEFT_1_PENALTY=1.302444
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=TTCGCTAAGGAAACAAGACAAG
PRIMER_RIGHT_1_SEQUENCE=GAGACAAGCGAGGAGGTTAAGA
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=290,22
PRIMER_LEFT_1_TM=58.698
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=1.3236
PRIMER_LEFT_2_PENALTY=1.302444
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=TTCGCTAAGGAAACAAGACAAG
PRIMER_RIGHT_2_SEQUENCE=GGAAGAAGCTTGCTACAGGAAA
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=229,22
PRIMER_LEFT_2_TM=58.698
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=1.3895
PRIMER_LEFT_3_PENALTY=1.302444
PRIMER_RIGHT_3_PENALTY=0.087031
PRIMER_LEFT_3_SEQUENCE=TTCGCTAAGGAAACAAGACAAG
PRIMER_RIGHT_3_SEQUENCE=ATTGGAAGAAGCTTGCTACAGG
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=232,22
PRIMER_LEFT_3_TM=58.698
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=232
PRIMER_PAIR_PENALTY_4=1.4102
PRIMER_LEFT_4_PENALTY=1.302444
PRIMER_RIGHT_4_PENALTY=0.107763
PRIMER_LEFT_4_SEQUENCE=TTCGCTAAGGAAACAAGACAAG
PRIMER_RIGHT_4_SEQUENCE=GACAAGCGAGGAGGTTAAGAAA
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=288,22
PRIMER_LEFT_4_TM=58.698
PRIMER_RIGHT_4_TM=59.892
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_00274 Ps_contig_mira-and-tgicl-ass_franssen_3123
SEQUENCE=ACAACAACTGTTTACTTATTTATTTATACATTAACATTTCAGGATTACATGACCATATAACTTCTTGCACACCCACAAAAGGAAACATTATAACCAAGAAAGCACACGATTTAAAGCAACAGAGTAACAGACTCAGTCTAAGTTTAACCAGAAATCTCAATAGACTTAACCTCAGCCTTCTTTATCTCTTTCTTTTGGAACTGTCACTGTGAGAACGCCGTTCNCCATGGGAAGCTTTCACTTTATCCTATTTTTA
TARGET=16,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00275 Ps_contig_mira-and-tgicl-ass_franssen_3126
SEQUENCE=ATTGTCTAACCTTTCTTGTTTCATCAAGCGCTATTTTTGTACTTATGTTCATCATTTGATATCTTGGTCTCTGGAATTACATCATTGAGGTGGACTTTTTAAGGACTATAAAGTACAGAATGGTAGGTACACATCATACAACATGCGCCCACCGGTTATTCTCGAACCATCCGCCACCTTTTCTTCTTCTTCTTCTCCAACCGATCGCAGGCTAAACACCCTCGCAAGACACCTTTCTCAATTTCCAGATCATAATCAAATGGCTTCTCATATTTCTCCTTCTCCAACAGCTTCTTCCGATTCCGTTTTCGCTCACATCGCTCAAGCCTCCCGAAGATCCTATCCTYGGGGTAACTGTTGCTTACAACAAAGATCCAAGTCCTAWTAAGCTCAACTTGGGCGTTGGTGCTTACCGAA
TARGET=181,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00276 Ps_contig_mira-and-tgicl-ass_franssen_3127
SEQUENCE=GATAAAGTAGGCGTACAGAAACCACACCTTTTCKACTTTACATTTTTTTAGTTAACTTTTAATTTAAATTTATTCAACCAAAACCAAACCAGAAAATTCATACACAGAATATATATACACCACCCCATCATACAACATGCGCCCACCGGTTATTCTCGAACCATCCGCCACCTTTTCTTCTTCTTCTTCTCCAACCGATCGCAGGCTAAACACCCTCGCAAGACACCTTTCNCAATTCCAGATCATAATCAATGGCTTCTCATATTCNCCTTCTCCAC
TARGET=175,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00277 Ps_contig_mira-and-tgicl-ass_franssen_3128
SEQUENCE=TCGGGKACCGGTTATTCTCGAACCATCCGCCACCTTTTCTTCTTCTTCTTCTCCAACCGATCGCAGGCTAAACACTCTCGCAAGACACCTTTCTCAATTTCCAGATCATAATCAAATGGCTTCTCATTATTTCCTTCCCTTTCCTACCCAAACAGTCCTTTCCTTCCGTATTTCCGTTTTCGCTCACNTCGCTCAAG
TARGET=37,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00278 Ps_contig_mira-and-tgicl-ass_franssen_3129
SEQUENCE=ACCAACGCCCAAGTTGAGCTTAATAGGACTTGGATCTTTGTTGTAAGCAACAGTTACCCCGAGGATAGGATCTTCGGGAGCTTGAGCGATGTGAGCGAAAACGGAATCGGAAGAAGCTGTTGGAGAAGGAGAAATATGAGAAGCCATTTGATTATGATCTGGAAATTGAGAAAGGTGTCTTGCGAGAGTGTTTAGCCTGCGATCGGTTGGAGAAGAAGAAGAAGAAAAGGTGGCGGATGGTTCGAGAATAACCGGTGGGCGCATGTTGTATGATGGGGTGGTGTATATATATTCTGTGTATGAATTTTCTGGTTTGGTTTTGGTTGAATAAATTTAAATTAAAAGTTAACTAAAAAAATGTAAAGTCCCCGAGTASTSTGGTTTCTGTACGCCTACTTTATCCCCGNGTA
TARGET=211,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00279 Ps_contig_mira-and-tgicl-ass_franssen_3130
SEQUENCE=CTCCTTTGTTGCGTAAAGAAACAAAGTCGTTCAAACAGTTGGTGTTCAACACGCCTCACTACATCCAACACAAGGGGTTTACCTTCCTCAGTTCGGTAAGCACCAACGCCCAAGTTGAGCTTAATTAGGACTTGGATTCTTTTGTTGTAAGCAACACGTTACCCCGAGGATAGGATCGTTCGGGAGCTTGAGCGATGTGAGACGAAAACGGAATCGGAAGAAGCTGGTTGGAAGAAGGAAGAAATATGAAGAAGCYATTTGATTATGATCGTAGGAAATTGGAAGGTAAAAGGTTGTTCTTGGCGAGGAGTTGTTTTACGCCCTGGCGAGTTCGGGTTAGGAAGAAAGAAAGAAAGAAAGGAAAAGGGTGGACGGAGT
TARGET=342,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00280 Ps_contig_mira-and-tgicl-ass_franssen_3143
SEQUENCE=TTAGGGTCAAGGAAAAAGGAAGAAAGTAATGGATGCTCGAGGACGTCATTACCAGAAAGGAGGAAGACAAAGNTCCACCGATTGTGTCATTAAGAAGACCCTACCGAACGGGAGTGATTGTAAAGAGCTCCGAACGAAGTAGGGTACGATGAGTCTGTGATGTAGAGAGTGAATGTGTTTGTATAGGAACTTGTTCTGATGCATGTACCTGTGCATAAAGTATTGCCATGTGTCCATTACCAACATAGCAGTAACAAATTGTCTTGCTAAAACAAGAATAGAAGCATTCTCATTGGTACTACTTTGTGTATCACTTCCTGTCACTGCAAATAAGAGGGTTGCAACCACAGCTTGAACAGCTTGTTGAAGGAGAACTCCTTTAACCACATCAAACTTAGAAACCAAATTCTTTTCATCCTCATCTTTTATTGTATGTAACCTATAATCTTCAGCAAACAACCCCAAAACAACGTAAAAACCAGAATAAATCCAATAAACTAAAAGTGGAGCAAAAGTCCCCAACATCTCATCAGAAATCTCCAAACCCATTTTCTCACACACCCAGAAGAAAAAATCAAATTTTTTTCTCCGATTTGATTTAGGTACAACCCACAAACCCCAAAATTTGCATGAATTTCAAAGTACCCAAAGAAACAAAAGGATTTAATAACAAAATTGGAAACCCTTAACCCTAACCCTCTTTATGGAAAATTTATAAAAAAAAAACCTTAAAATTGAAACTCTCCCTCTTCTTGTTCCTCTAACCCTAATGATTGAATTCAAACCCAAAACCAATAACAACGCGATTAAATATTGGATTAGGTTTCCAGGAAGGTTCCAAAGAGAGAAGAGAGAGAGAATGTTGAAAACTGTTTTTCCT
TARGET=849,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00281 Ps_contig_mira-and-tgicl-ass_franssen_3144
SEQUENCE=ATTTCCTCAAGGACCCTGTGAAATACGATGTTAAATATCAGGCTAGTGCACAACCGTTTTTGGTTCTTGGTCTGTTGGTTACTGCTCTAGCAACAAGCCATTGATAAAAAGAAGTTCAAGAGAGAGAGAGTCACACCAGTTAGAAATAATTTCATAAATTTGTATTGAAGATGTGTGCCATAGTATTAATGTAATTTCATTTTTCTTGTTTTTTCCTTCTAATTTTTGTTTTGTTCTATCTTCTCTTATCCACTTAATTTTTTTTAAAGTATTTCTAAGCTCAGGTTGAGAAAAGATTTATTTTTAGGAAACTATAAATAAGACCAATTGTACAAATGTATTGTAGACATATTCGCATATACAATTTTCCTTCCATAGTTYAATAAATAATTAAAACATTGGCTACCTATCATGGTTTTATTAAGTT
TARGET=119,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00282 Ps_contig_mira-and-tgicl-ass_franssen_3157
SEQUENCE=ACAAGAGGGTGCCCTGGGAACAAAGAAGCAACGTTKTSCCYATTCAGGGCCCATGCCAAGCAACATGAGATCAAATTTTGGAAATCCACTGGTTGATGATGATGTTATCACATTGCTATTGACCAAGTGTTTGACACATGTCTCGTAATCATCAGCTGCTCCTTCAGCTGGAAAGGGCTCATTGATTGACAATAAACAGTTCGGCCCGGGGTTAGGGAAAGGTTCGGGGGTAACCCGTTTGGGGAAGGAAGGAAAACGGCCCAA
TARGET=243,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00283 Ps_contig_mira-and-tgicl-ass_franssen_3178
SEQUENCE=AGKAAAGAAGCAAAGATTCATAGTTCATTGCAGAAAATTTCATAATAAAACAACAGAAACAATTGAAAGAAAGTAAAAATAATGTTACAACTTAAGAGTTTCGCAAATAAGGATAGAAAAACCTTTTACTGAAAATGTTTAGAAGTGATTGAACATTATACATAGCAATGGCACATTTAAAGTGATACAGATACTAGTACATAACCACTAAAGCACAACCAACCCACAAGAAGCATCAGGAGTTGTTCCTTTTGCGAGCTATCTCCCTCGCCATCTCTCTAAGCTCCTCTGGTGGTGGTGGCTTTTGTGTATCTTTGGGATATTCAACGTATGATTTTTTTCCGATGAACTTGTGGAGGGTGTTGTCGGTATTGCTAGCATAAACGTACATAAGAACGATGGGTACAGTAACGTAAACCCCAAACTTGGCGATCTCTAGAATCCCCTTTGAAGTTCCCAGTGATGACATCTTTCCTTTCCTTCCGCCAACAAACTCAACTAACAACTCAGATCAAATCACACACCTCTCAATTTAGATCAAACCATATGGGTGTTGCAATAAATTAAATTGACCCAGCCAGTTGAATTGAGAGGATAGTGTGAACCTAATATGACCTCTGCTCCCGGTATCTCACGTGTTCCACGTGATTATACTATAGTGGACCGAGATCCCCGAC
TARGET=290,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00284 Ps_contig_mira-and-tgicl-ass_franssen_3183
SEQUENCE=TAATATCGGGACAATCAGGTGCCGTTGATCGATCGATCTGGGAGAAGAATGGGAGCAGGAAAGAATACGGCAGAGTTCTTCAGGAGGAGAGACGAATGGAGGAAGCATCCGATGCTTGGGAATCAGTTTCGTCACGCTACTCCTGGCCTCGGTATCGCTCTCGTTGCGTTTGGTGTTTACCTCGTCGCTGAGCAAGTTTACAACAAGGCCAATGCTTCTTCCTCTTCTCACCATGTGAAGGCGTGGGGAGCACTGAAGGAAAATCAACTAATTCATCTATTTTCCTTTGTTTTGTTTTGGTTTAAGTTGTTTAGTTAAATTTAAACGG
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1195
PRIMER_LEFT_PENALTY=1.057682
PRIMER_RIGHT_PENALTY=0.061818
PRIMER_LEFT_SEQUENCE=TAATATCGGGACAATCAGGTGC
PRIMER_RIGHT_SEQUENCE=AGCATTGGCCTTGTTGTAAACT
PRIMER_LEFT=0,22
PRIMER_RIGHT=215,22
PRIMER_LEFT_TM=61.058
PRIMER_RIGHT_TM=60.062
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=1.1671
PRIMER_LEFT_1_PENALTY=1.057682
PRIMER_RIGHT_1_PENALTY=0.109391
PRIMER_LEFT_1_SEQUENCE=TAATATCGGGACAATCAGGTGC
PRIMER_RIGHT_1_SEQUENCE=CTTGTTGTAAACTTGCTCAGCG
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=206,22
PRIMER_LEFT_1_TM=61.058
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=1.1743
PRIMER_LEFT_2_PENALTY=1.057682
PRIMER_RIGHT_2_PENALTY=0.116582
PRIMER_LEFT_2_SEQUENCE=TAATATCGGGACAATCAGGTGC
PRIMER_RIGHT_2_SEQUENCE=GTTGATTTTCCTTCAGTGCTCC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=61.058
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=1.2137
PRIMER_LEFT_3_PENALTY=1.057682
PRIMER_RIGHT_3_PENALTY=0.156061
PRIMER_LEFT_3_SEQUENCE=TAATATCGGGACAATCAGGTGC
PRIMER_RIGHT_3_SEQUENCE=TCACATGGTGAGAAGAGGAAGA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=237,22
PRIMER_LEFT_3_TM=61.058
PRIMER_RIGHT_3_TM=59.844
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=1.4399
PRIMER_LEFT_4_PENALTY=1.057682
PRIMER_RIGHT_4_PENALTY=0.382228
PRIMER_LEFT_4_SEQUENCE=TAATATCGGGACAATCAGGTGC
PRIMER_RIGHT_4_SEQUENCE=ATGGTGAGAAGAGGAAGAAGCA
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=61.058
PRIMER_RIGHT_4_TM=60.382
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_00285 Ps_contig_mira-and-tgicl-ass_franssen_3185
SEQUENCE=GAGAGAAATTTATGATAAATGGTAGAATTAATTTTGATTTAAAATCAATAACATTTTATGGAGTTATTATTATATGCTGGCGGGAGAATCTGTCCAGATTTGGAAGAGAGAAAAAAAAGTTTATTTTTATTTATTTATAAGTAAAATTTTGTTGATTCCATAAAAAAAATTAGTTTATGGTGTTTGTTGTGCACATGGTTTGGTTTCGAATTGTTATTTGCAATGTGAAAGGTAGGTGTTGTTTTTTTTAACCTT
TARGET=126,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5153
PRIMER_LEFT_PENALTY=0.177514
PRIMER_RIGHT_PENALTY=0.337818
PRIMER_LEFT_SEQUENCE=ATTATTATATGCTGGCGGGAGA
PRIMER_RIGHT_SEQUENCE=CATGTGCACAACAAACACCATA
PRIMER_LEFT=65,22
PRIMER_RIGHT=196,22
PRIMER_LEFT_TM=59.822
PRIMER_RIGHT_TM=60.338
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.6380
PRIMER_LEFT_1_PENALTY=0.177514
PRIMER_RIGHT_1_PENALTY=0.460467
PRIMER_LEFT_1_SEQUENCE=ATTATTATATGCTGGCGGGAGA
PRIMER_RIGHT_1_SEQUENCE=CAACACCTACCTTTCACATTGC
PRIMER_LEFT_1=65,22
PRIMER_RIGHT_1=240,22
PRIMER_LEFT_1_TM=59.822
PRIMER_RIGHT_1_TM=59.540
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=0.9284
PRIMER_LEFT_2_PENALTY=0.177514
PRIMER_RIGHT_2_PENALTY=0.750851
PRIMER_LEFT_2_SEQUENCE=ATTATTATATGCTGGCGGGAGA
PRIMER_RIGHT_2_SEQUENCE=AAACCATGTGCACAACAAACAC
PRIMER_LEFT_2=65,22
PRIMER_RIGHT_2=200,22
PRIMER_LEFT_2_TM=59.822
PRIMER_RIGHT_2_TM=60.751
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=1.0955
PRIMER_LEFT_3_PENALTY=0.757635
PRIMER_RIGHT_3_PENALTY=0.337818
PRIMER_LEFT_3_SEQUENCE=TTATTATATGCTGGCGGGAGAA
PRIMER_RIGHT_3_SEQUENCE=CATGTGCACAACAAACACCATA
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=196,22
PRIMER_LEFT_3_TM=60.758
PRIMER_RIGHT_3_TM=60.338
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=1.2181
PRIMER_LEFT_4_PENALTY=0.757635
PRIMER_RIGHT_4_PENALTY=0.460467
PRIMER_LEFT_4_SEQUENCE=TTATTATATGCTGGCGGGAGAA
PRIMER_RIGHT_4_SEQUENCE=CAACACCTACCTTTCACATTGC
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=240,22
PRIMER_LEFT_4_TM=60.758
PRIMER_RIGHT_4_TM=59.540
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_00286 Ps_contig_mira-and-tgicl-ass_franssen_3237
SEQUENCE=ATTTTTAGCTCAAAGGAAGAAGGTTCTTGAGGGAAAGGAGTGAAGTGGGTTTTGTAACGAGTTTTGTTGGGTTGTGGAACTTTGGTGAACTAGGTAGTGTTGAAGCACTTTGTGTTCATCATGGTCATTGCGATTATGGTTGATGCCATTGATTGATTGATGAATGAGTAGAAGTGTCTTTGTTTGAGNGTGTAGAATGATGTGTGGTNTTTGCGTAGGTTAGG
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1400
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.102638
PRIMER_LEFT_SEQUENCE=AAGCACTTTGTGTTCATCATGG
PRIMER_RIGHT_SEQUENCE=TAACCTACGCAAANACCACACA
PRIMER_LEFT=102,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.103
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=0.1679
PRIMER_LEFT_1_PENALTY=0.065292
PRIMER_RIGHT_1_PENALTY=0.102638
PRIMER_LEFT_1_SEQUENCE=TGTAACGAGTTTTGTTGGGTTG
PRIMER_RIGHT_1_SEQUENCE=TAACCTACGCAAANACCACACA
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=221,22
PRIMER_LEFT_1_TM=59.935
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.2046
PRIMER_LEFT_2_PENALTY=0.102004
PRIMER_RIGHT_2_PENALTY=0.102638
PRIMER_LEFT_2_SEQUENCE=AAGAAGGTTCTTGAGGGAAAGG
PRIMER_RIGHT_2_SEQUENCE=TAACCTACGCAAANACCACACA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=221,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=60.103
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.2046
PRIMER_LEFT_3_PENALTY=0.102004
PRIMER_RIGHT_3_PENALTY=0.102638
PRIMER_LEFT_3_SEQUENCE=AAAGGAAGAAGGTTCTTGAGGG
PRIMER_RIGHT_3_SEQUENCE=TAACCTACGCAAANACCACACA
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=221,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.103
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=0.2448
PRIMER_LEFT_4_PENALTY=0.142170
PRIMER_RIGHT_4_PENALTY=0.102638
PRIMER_LEFT_4_SEQUENCE=TTCTTGAGGGAAAGGAGTGAAG
PRIMER_RIGHT_4_SEQUENCE=TAACCTACGCAAANACCACACA
PRIMER_LEFT_4=23,22
PRIMER_RIGHT_4=221,22
PRIMER_LEFT_4_TM=59.858
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_00287 Ps_contig_mira-and-tgicl-ass_franssen_3255
SEQUENCE=TTTAGGAAATACTCTCCTGGCCAGCTGTATCCCAAATCTGTAAGGTGAAAAGCCTATCTTCAAATTGCACTTCTTTGGTTAAGAAATCCGCTCCAATGGTTGCCTTGTACTGATTACTAAACTTCTTAATTCACATATTGGTTCATCAAAGACGTCTTCCCCACACCGCTGTCACCGAGAATGATGACCTTTAAGAGAGTTCTTCTGCGAGAAGGCATAGTACGGATCGGTGGAAGAAGAAGAAACTGTTTGCTTGTGTTTGATGATTCTTTTTTTACTTCAACTAGGACTCTCTCACACCGTCTTCAACTCTATTTCTCTCTCTCTAACTTTCTAAGCAGCCGYCTTTCTCTTTCTATCTACAAATTCTTGAGRACGG
TARGET=233,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00288 Ps_contig_mira-and-tgicl-ass_franssen_3255
SEQUENCE=TTTAGGAAATACTCTCCTGGCCAGCTGTATCCCAAATCTGTAAGGTGAAAAGCCTATCTTCAAATTGCACTTCTTTGGTTAAGAAATCCGCTCCAATGGTTGCCTTGTACTGATTACTAAACTTCTTAATTCACATATTGGTTCATCAAAGACGTCTTCCCCACACCGCTGTCACCGAGAATGATGACCTTTAAGAGAGTTCTTCTGCGAGAAGGCATAGTACGGATCGGTGGAAGAAGAAGAAACTGTTTGCTTGTGTTTGATGATTCTTTTTTTACTTCAACTAGGACTCTCTCACACCGTCTTCAACTCTATTTCTCTCTCTCTAACTTTCTAAGCAGCCGYCTTTCTCTTTCTATCTACAAATTCTTGAGRACGG
TARGET=317,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00289 Ps_contig_mira-and-tgicl-ass_franssen_3277
SEQUENCE=GTCGGRKATTGAAGGTGAGTAMGTCGGGGAGAGATTCAAACATCTCTTCTTCTTTCCCTCACTCTACCCTCTCCTCTAAAAAAAATTCACTTTTTAATAGAATAATATTTTCAAGATTAATTTGTGCATCCTCTTTCAGTTTTCAAGAACATGTTTTTGATAGTTCCTTTCCAAATATGCACCTAGAGAGAGAGAGAAACAATAGCAAGGATCAATCTTCATTTGTGATATAGCTATTGAAGAAAGATTAGTAGAATTTTTGGCTTGCAATGATATGGCTTTCTTTCCATCAAATTTCATGCTTCAAACTTCTCATCAAGATGACCATCATCAACCTCCACCTTCACTCAACTCAATTATAACCTCATGCGCACCTCAAGATTACCATGGAGGAGTATCCTTTTTGGGAAAGAGATCTATGTCGTTTTCCGGGATAGAACTCGGAGAAGAAGCGAATGTGGAAGAAGAATTATCGGACGACGGATCGCAGTTAGGGGAGAAGAAGAGAAGGCTTAACATGGAACAAGTGAAGACCCTTGAGAAAAGCTTTGAGTTAGGAAACAAGCTTGAACCTGAGAGGAAAATGCAGCTTGCAAGGGCTCTTGGACTTCAACCAAGACAAATTGCTATATGGTTTCAAAACAGAAGGGCTAGATGGAAGACAAAACAGTTGGAGAAAGACTATGATGTTCTTAAAAGACAATATGATTCTGTTAAGGCTGATAATGATGCACTTCAAGCTCAGAACCAGAAGCTTCAGTCAGAGATATTGGCACTGAAAAATAGAGAACCAACAGAATCAATCAACCTAAACAAAGAAACAGAAGGATCAAGCAGCAACAGAAGTGAAAACAGTTCAGAAATCAAGTTGGATATTTCAACAAGGACGACACAAACAGCTATTGATAGTCCTTTATCCACTCAACAACAAACAAGCATAAACCTCTTTCCATCTTCTTCTAGACCAACACTAGTTCCTCATCAACTCTTTCAAACCAATTCGACAAGACAAGACATTAATCAGTGCCAAAAGATTGATCACATGGTTAAAGAAGAGAGTTTGAGTAACATGTTTTCTGCCATTGATGATCAATCTGGATTATGGCCATGGTTGGAGCAACAACACTTCAATTGATCAAATAAAAGAGTTTTCAGATTTAAAATTTTCTTGATTATCATACTCGCGCATGTTCGGATAAACAACGACTTTATGTCGTAGTGATTTTGACAAAAATACGCTATGAACTTTTAATAAAATCACAGTGTCGCTGTAATTTTGTCGAATTTACTGTCGATTTATTGAGTTGCGCAAGTTATAAACTTTGTTTTGGTGAACTTGATGTATAAAATAGGATATATATCAAGCATAATTTTTCAAGTTGAAGTTGAAATATATATATTCTTTCCAGTAATTAGTGAAATATTGCAGATTTGCAACTTTKTAAAAAA
TARGET=185,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00290 Ps_contig_mira-and-tgicl-ass_franssen_3277
SEQUENCE=GTCGGRKATTGAAGGTGAGTAMGTCGGGGAGAGATTCAAACATCTCTTCTTCTTTCCCTCACTCTACCCTCTCCTCTAAAAAAAATTCACTTTTTAATAGAATAATATTTTCAAGATTAATTTGTGCATCCTCTTTCAGTTTTCAAGAACATGTTTTTGATAGTTCCTTTCCAAATATGCACCTAGAGAGAGAGAGAAACAATAGCAAGGATCAATCTTCATTTGTGATATAGCTATTGAAGAAAGATTAGTAGAATTTTTGGCTTGCAATGATATGGCTTTCTTTCCATCAAATTTCATGCTTCAAACTTCTCATCAAGATGACCATCATCAACCTCCACCTTCACTCAACTCAATTATAACCTCATGCGCACCTCAAGATTACCATGGAGGAGTATCCTTTTTGGGAAAGAGATCTATGTCGTTTTCCGGGATAGAACTCGGAGAAGAAGCGAATGTGGAAGAAGAATTATCGGACGACGGATCGCAGTTAGGGGAGAAGAAGAGAAGGCTTAACATGGAACAAGTGAAGACCCTTGAGAAAAGCTTTGAGTTAGGAAACAAGCTTGAACCTGAGAGGAAAATGCAGCTTGCAAGGGCTCTTGGACTTCAACCAAGACAAATTGCTATATGGTTTCAAAACAGAAGGGCTAGATGGAAGACAAAACAGTTGGAGAAAGACTATGATGTTCTTAAAAGACAATATGATTCTGTTAAGGCTGATAATGATGCACTTCAAGCTCAGAACCAGAAGCTTCAGTCAGAGATATTGGCACTGAAAAATAGAGAACCAACAGAATCAATCAACCTAAACAAAGAAACAGAAGGATCAAGCAGCAACAGAAGTGAAAACAGTTCAGAAATCAAGTTGGATATTTCAACAAGGACGACACAAACAGCTATTGATAGTCCTTTATCCACTCAACAACAAACAAGCATAAACCTCTTTCCATCTTCTTCTAGACCAACACTAGTTCCTCATCAACTCTTTCAAACCAATTCGACAAGACAAGACATTAATCAGTGCCAAAAGATTGATCACATGGTTAAAGAAGAGAGTTTGAGTAACATGTTTTCTGCCATTGATGATCAATCTGGATTATGGCCATGGTTGGAGCAACAACACTTCAATTGATCAAATAAAAGAGTTTTCAGATTTAAAATTTTCTTGATTATCATACTCGCGCATGTTCGGATAAACAACGACTTTATGTCGTAGTGATTTTGACAAAAATACGCTATGAACTTTTAATAAAATCACAGTGTCGCTGTAATTTTGTCGAATTTACTGTCGATTTATTGAGTTGCGCAAGTTATAAACTTTGTTTTGGTGAACTTGATGTATAAAATAGGATATATATCAAGCATAATTTTTCAAGTTGAAGTTGAAATATATATATTCTTTCCAGTAATTAGTGAAATATTGCAGATTTGCAACTTTKTAAAAAA
TARGET=1391,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00291 Ps_contig_mira-and-tgicl-ass_franssen_3284
SEQUENCE=CCTTAACGTCCAACCAACGTTCTAAACCAACCAAACCAATTTACCTAATACCCTAAAAATTTAACCTAATTCCAATACCTTTGTATNTTTAAAAAAAAGGTAACAAAATAACTAAGCAGACCCATGACACGAATTATTCAATAACACCCTAAAAACGAACGACTTGAGAAAAAAGTAACAAAATAGAAAATAATAGTAAAAGCCATGAATGAAAGGCTGCGATTTGATGATGATGATGATAACGATCACTTTTTGAGGCTTACCTACGTAACTACCTAGGGCGAGGCTTGGAAGATTCCGGAACAGCAGCTGCAGATGCGTGACGATTCACGAGCTTATCTTCCTCGAAAGGGGATTCGTTCGGGTGACGAGCATCGTGATGAATCTGCATCGTATTTCACATCGTAGGTTACCGTTTGTTTGCGTAGGT
TARGET=225,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0467
PRIMER_LEFT_PENALTY=0.035021
PRIMER_RIGHT_PENALTY=0.011696
PRIMER_LEFT_SEQUENCE=ACCCTAAAAACGAACGACTTGA
PRIMER_RIGHT_SEQUENCE=CGATGTGAAATACGATGCAGAT
PRIMER_LEFT=145,22
PRIMER_RIGHT=404,22
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.0467
PRIMER_LEFT_1_PENALTY=0.035021
PRIMER_RIGHT_1_PENALTY=0.011696
PRIMER_LEFT_1_SEQUENCE=ACCCTAAAAACGAACGACTTGA
PRIMER_RIGHT_1_SEQUENCE=AATACGATGCAGATTCATCACG
PRIMER_LEFT_1=145,22
PRIMER_RIGHT_1=396,22
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=59.988
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.1034
PRIMER_LEFT_2_PENALTY=0.035021
PRIMER_RIGHT_2_PENALTY=0.068420
PRIMER_LEFT_2_SEQUENCE=ACCCTAAAAACGAACGACTTGA
PRIMER_RIGHT_2_SEQUENCE=ATCCCCTTTCGAGGAAGATAAG
PRIMER_LEFT_2=145,22
PRIMER_RIGHT_2=355,22
PRIMER_LEFT_2_TM=60.035
PRIMER_RIGHT_2_TM=59.932
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.1153
PRIMER_LEFT_3_PENALTY=0.035021
PRIMER_RIGHT_3_PENALTY=0.080323
PRIMER_LEFT_3_SEQUENCE=ACCCTAAAAACGAACGACTTGA
PRIMER_RIGHT_3_SEQUENCE=CAAACGGTAACCTACGATGTGA
PRIMER_LEFT_3=145,22
PRIMER_RIGHT_3=418,22
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.1576
PRIMER_LEFT_4_PENALTY=0.035021
PRIMER_RIGHT_4_PENALTY=0.122569
PRIMER_LEFT_4_SEQUENCE=ACCCTAAAAACGAACGACTTGA
PRIMER_RIGHT_4_SEQUENCE=CGAGGAAGATAAGCTCGTGAAT
PRIMER_LEFT_4=145,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=60.035
PRIMER_RIGHT_4_TM=59.877
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_00292 Ps_contig_mira-and-tgicl-ass_franssen_3285
SEQUENCE=CTTCCAAGCCTCGCCCTGGTGTTCGTGGTAGTTCCTTCTAAAAAGTTGTATCGTTATCGTCATCATCATCAAATCGCAGCCTTTCATTCATGGCTTTTACTATATTTTCTATTTTTGTACTTTTTTTTCTTTCAAGTCGTCGTTTTTAGGGTGTTATTGAATAATTCGTGTCATGGGTCTGCTTAGTTATTTTGTCCTTTTTTTTTTAAATATCAAGATGATGTAATTTTAGGTATGAATGTTGTGTTGAACTGTTGCGATGTAAAGGTTTTGATGGATCGGNTT
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4827
PRIMER_LEFT_PENALTY=0.447703
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=CTGGTGTTCGTGGTAGTTCCTT
PRIMER_RIGHT_SEQUENCE=ACCCTAAAAACGACGACTTGAA
PRIMER_LEFT=15,22
PRIMER_RIGHT=151,22
PRIMER_LEFT_TM=60.448
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=0.4880
PRIMER_LEFT_1_PENALTY=0.453004
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=GGTGTTCGTGGTAGTTCCTTCT
PRIMER_RIGHT_1_SEQUENCE=ACCCTAAAAACGACGACTTGAA
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=151,22
PRIMER_LEFT_1_TM=59.547
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=0.6132
PRIMER_LEFT_2_PENALTY=0.447703
PRIMER_RIGHT_2_PENALTY=0.165541
PRIMER_LEFT_2_SEQUENCE=CTGGTGTTCGTGGTAGTTCCTT
PRIMER_RIGHT_2_SEQUENCE=CCTTTACATCGCAACAGTTCAA
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=60.448
PRIMER_RIGHT_2_TM=60.166
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.6185
PRIMER_LEFT_3_PENALTY=0.453004
PRIMER_RIGHT_3_PENALTY=0.165541
PRIMER_LEFT_3_SEQUENCE=GGTGTTCGTGGTAGTTCCTTCT
PRIMER_RIGHT_3_SEQUENCE=CCTTTACATCGCAACAGTTCAA
PRIMER_LEFT_3=17,22
PRIMER_RIGHT_3=267,22
PRIMER_LEFT_3_TM=59.547
PRIMER_RIGHT_3_TM=60.166
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=0.6648
PRIMER_LEFT_4_PENALTY=0.447703
PRIMER_RIGHT_4_PENALTY=0.217054
PRIMER_LEFT_4_SEQUENCE=CTGGTGTTCGTGGTAGTTCCTT
PRIMER_RIGHT_4_SEQUENCE=TACATCGCAACAGTTCAACACA
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=263,22
PRIMER_LEFT_4_TM=60.448
PRIMER_RIGHT_4_TM=60.217
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_00293 Ps_contig_mira-and-tgicl-ass_franssen_3286
SEQUENCE=GAACACCGTTTCATTCATCAACTCAATCCGATTCCCATCAAAACCTTTCATCGCAACAGTTCAAACACAACATTCATACCTAAAATTCATCATCTTGAATAATTTAAAAAAAAAGGACAAAATAACTAAGCAGACCCATGAACACGAATTATTCAATAACCAACCCTAAAAACGACGACTTAGAAAGAAAAAAAGTAACAAAATAAGAAAATATAAGTAAAAGCCATGAATGAAAGGCTGCGTATTTGATGATGATGATGATAACGATCTTCTTTTGGACGGCCTAACCCG
TARGET=247,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8970
PRIMER_LEFT_PENALTY=0.081185
PRIMER_RIGHT_PENALTY=0.815854
PRIMER_LEFT_SEQUENCE=ATCGCAACAGTTCAAACACAAC
PRIMER_RIGHT_SEQUENCE=GTTAGGCCGTCCAAAAGAAGAT
PRIMER_LEFT=49,22
PRIMER_RIGHT=287,22
PRIMER_LEFT_TM=60.081
PRIMER_RIGHT_TM=60.816
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=1.1630
PRIMER_LEFT_1_PENALTY=0.081185
PRIMER_RIGHT_1_PENALTY=1.081865
PRIMER_LEFT_1_SEQUENCE=ATCGCAACAGTTCAAACACAAC
PRIMER_RIGHT_1_SEQUENCE=TTAGGCCGTCCAAAAGAAGAT
PRIMER_LEFT_1=49,22
PRIMER_RIGHT_1=286,21
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=60.082
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=1.3381
PRIMER_LEFT_2_PENALTY=0.522246
PRIMER_RIGHT_2_PENALTY=0.815854
PRIMER_LEFT_2_SEQUENCE=AAACCTTTCATCGCAACAGTTC
PRIMER_RIGHT_2_SEQUENCE=GTTAGGCCGTCCAAAAGAAGAT
PRIMER_LEFT_2=40,22
PRIMER_RIGHT_2=287,22
PRIMER_LEFT_2_TM=60.522
PRIMER_RIGHT_2_TM=60.816
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=1.4758
PRIMER_LEFT_3_PENALTY=0.659953
PRIMER_RIGHT_3_PENALTY=0.815854
PRIMER_LEFT_3_SEQUENCE=GAACACCGTTTCATTCATCAAC
PRIMER_RIGHT_3_SEQUENCE=GTTAGGCCGTCCAAAAGAAGAT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=287,22
PRIMER_LEFT_3_TM=59.340
PRIMER_RIGHT_3_TM=60.816
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=1.4876
PRIMER_LEFT_4_PENALTY=0.671790
PRIMER_RIGHT_4_PENALTY=0.815854
PRIMER_LEFT_4_SEQUENCE=ATAACCAACCCTAAAAACGACG
PRIMER_RIGHT_4_SEQUENCE=GTTAGGCCGTCCAAAAGAAGAT
PRIMER_LEFT_4=155,22
PRIMER_RIGHT_4=287,22
PRIMER_LEFT_4_TM=59.328
PRIMER_RIGHT_4_TM=60.816
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_00294 Ps_contig_mira-and-tgicl-ass_franssen_3287
SEQUENCE=CAAAAAGTGATCGTTATCATCATCATCAAATCGCAGCCTTTCATTCATGGCTTTTACTATATTTTCTATTTTGTACTTTTTTTCTTTCAAGTCGTCGTTTTTAGGGTGTTATTGAATAATTCGTGTCATGGGTCTRCTTAGTTATTYTGTCCTTTTTTTAAAATATCAAGATGATGTAATTTTAGGTATGAATGTTGTGTTTGAACTTGTTGCGATGTAAAGGTTTTGAGTGGGAATCGGATTGAGTTGTGCTTGTTAATTTAATTTAAGTTTCGGKCCCCTTTACTTAACGTCC
TARGET=16,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00295 Ps_contig_mira-and-tgicl-ass_franssen_3311
SEQUENCE=ATAATACTATTAATTAATTAAATTTAATACGTTAAGCTTTCTCAGCCGCTACGTTCTTCACCACAGCCGTCACAGCCAGCGTGTTCTTTTGTTCTAATCCCATTCATTAATAATTTACCTGTTTAATTATCACTAATCCTCAATTACAACTCTTTATTCTCATTCTATTAATTAACTAATCTTTTCTCTAGAAAACGATTAATACCGATTTGTCACCTTTCGCCGTTTCTTCCTCTCTCTTTCATCATAACCTTTCCGACTCTGTTATCTTTCCCCGTTAACATTCTTTCTCTTCAATTTCACCTTTCTTATCTCTTCCTCTCAATTCCTCTCAATTCCTCTCAATTCCTCAACCACTCCTTCTCTTCAATCAGGTTTTTTTTCATCTGAAAGAACAGATCTACCTGAATCTATGGCGGAAGAACACCGATGCGAAACTCCGGAAGGTCACAGACTCTGCGCTAACAACTGCGGCTTCTTCGGGAGCTCAGCTACGATGAATTTCTGTTCAAAATGTTACCGAGACATTCATTTGAAGGAGCAAGAACAAGCCAAAACCAAATCCACAATCGAAACCGCTCTTTCTTCTGCCTCCTCCTCCGCCGCTACCGTAGTTGTTGAATCTGCGGTTCTAGAGATCGAATCTCTCCCTCAACCACCGGTACCGTCGATCGTGACTAACGCGTCGGAATCTTCGATCGGATCGGTTCAACCAAACCGGTGCGGGACGTGTAGAAAACGGACCGGGTTAACCGGATTCAAGTGCAGATGTGGAATCACGTTTTGTGGGACCCACAGGTATCCGGAGAAACACGAGTGCGGTTTCGATTTCAAGGCGGTGGGAAGAGAGGAGATAGCTAAAGCGAATCCTGTGATCAAAGCCGATAAGCTGAGAAGAATTTGATTTGATTTCCGCCGTTTGATGTGATTTCGATGATTTCATCTGGACCGTTCGCTGGGGAACTTACCTACACAAACGAATCATGTGGCGACGCTTGATGAAATGATGTAAAATAATCTATCTAATTAAAAAAGGTTGAATTAARTTTAAGTTTTTTTACCG
TARGET=9,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00296 Ps_contig_mira-and-tgicl-ass_franssen_3312
SEQUENCE=GTTTCTTAAACAAAAGTCCTAAACAAGTAGAATGTAAAATAACATAACTTACAAAACCTAACTTCTTCCTAATTTTGCTACTAAACAAAAAACCAATAAAATAGTCTTTTTTTTTCCTAACAAAAGTAATTTACAAATAACTGTAATAACAGAACACCATCCACAAAATCCAAGAAGAATTTAACAAGTAAAAAAACAACTTTTCCATTCCCAAACTCACGTGTGTTTATGAAATCTAGATCCTCATACAAAAACCATAGTCGATAACCTCTCCCCGAGCGGAGCTCGCTCGCGGAGAGGATCCTAACGTCGGTTTTCGAAACAAGAACACACATCTAAAAAAGTTGTAGGGATATGGTTTGGGGAGAATGTAATGTGTCCATGATAAGAGAAGAAAAACAAAACATCCAAAACTTGTGAGGAATAAAATTCATGTTTCCAACCATACTCCAAACTTGGACTTGGGATCAGAAATCAAAGGATCATAACCCTTTATACATTCTTACGGATTAACATTGATAGGATCGGTTCGACCCTGCACGGCCATCCTCTCCGAGACATCCGCTAAAGAACTCAGGTTGCACCTGATCAACGCCTCTACGAAGTAACACGTTTCATCTCTCGTGTTCCCTTCAGGCACGTCCACGACGAACGATTCAATCACCATTGTACCTGGTCTTCCATCAATAACCTCCGGATGGACAGTGATTACGGAGGAATAGTTCCTGAGCCTATGATCRCCACCAACAATCCTGATACCGAGAATATGTTCTTCATCATCAAGCTGTTCCAACCTCTCAGTACTAGTTGTAGCCGGAAGACCAGATTTAACATTCACTTCTCTAACACTTCCAATGCCAAGATCACCTTGCATAATACACCTGCTAACAAATGGTTTATATTTCTGTGGCTGATCAAATCTTCTAACCAGAGACCAAACAAGATGAACAGGGGCTTTGATGTGTTTAACAAGTGCAGAGGAACACTGRTTGTCTCTTAAATCATGTTTGTGAYGTCTTCTTATGTACTGTGTCTCAATAGCAGAGTATTGTTCACCACCGTTGTTCATCTTGTTGGATGATTATTGTTTTGGTGGCTAGAACAAAAGAAAAAGAAGAAGAAGAATAAGAAGATAGAACAAGTGGTGGAGAGGGATAAGCTTGAGAGAGAGAGAGAGTAGWKRGWGTARARAGTGTGTAAGTAAATA
TARGET=1112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00297 Ps_contig_mira-and-tgicl-ass_franssen_3312
SEQUENCE=GTTTCTTAAACAAAAGTCCTAAACAAGTAGAATGTAAAATAACATAACTTACAAAACCTAACTTCTTCCTAATTTTGCTACTAAACAAAAAACCAATAAAATAGTCTTTTTTTTTCCTAACAAAAGTAATTTACAAATAACTGTAATAACAGAACACCATCCACAAAATCCAAGAAGAATTTAACAAGTAAAAAAACAACTTTTCCATTCCCAAACTCACGTGTGTTTATGAAATCTAGATCCTCATACAAAAACCATAGTCGATAACCTCTCCCCGAGCGGAGCTCGCTCGCGGAGAGGATCCTAACGTCGGTTTTCGAAACAAGAACACACATCTAAAAAAGTTGTAGGGATATGGTTTGGGGAGAATGTAATGTGTCCATGATAAGAGAAGAAAAACAAAACATCCAAAACTTGTGAGGAATAAAATTCATGTTTCCAACCATACTCCAAACTTGGACTTGGGATCAGAAATCAAAGGATCATAACCCTTTATACATTCTTACGGATTAACATTGATAGGATCGGTTCGACCCTGCACGGCCATCCTCTCCGAGACATCCGCTAAAGAACTCAGGTTGCACCTGATCAACGCCTCTACGAAGTAACACGTTTCATCTCTCGTGTTCCCTTCAGGCACGTCCACGACGAACGATTCAATCACCATTGTACCTGGTCTTCCATCAATAACCTCCGGATGGACAGTGATTACGGAGGAATAGTTCCTGAGCCTATGATCRCCACCAACAATCCTGATACCGAGAATATGTTCTTCATCATCAAGCTGTTCCAACCTCTCAGTACTAGTTGTAGCCGGAAGACCAGATTTAACATTCACTTCTCTAACACTTCCAATGCCAAGATCACCTTGCATAATACACCTGCTAACAAATGGTTTATATTTCTGTGGCTGATCAAATCTTCTAACCAGAGACCAAACAAGATGAACAGGGGCTTTGATGTGTTTAACAAGTGCAGAGGAACACTGRTTGTCTCTTAAATCATGTTTGTGAYGTCTTCTTATGTACTGTGTCTCAATAGCAGAGTATTGTTCACCACCGTTGTTCATCTTGTTGGATGATTATTGTTTTGGTGGCTAGAACAAAAGAAAAAGAAGAAGAAGAATAAGAAGATAGAACAAGTGGTGGAGAGGGATAAGCTTGAGAGAGAGAGAGAGTAGWKRGWGTARARAGTGTGTAAGTAAATA
TARGET=1163,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00298 Ps_contig_mira-and-tgicl-ass_franssen_3323
SEQUENCE=TAWGCATGATGATGATGATGATGAGAGAGAGAGAGAGGTTGAAGGTGGATGGCATGGTGTGGGGTCGGAGATGAAAGGTTGATGATGATTAAGACCGTGCGGGAAAGGGTTAATTAATGAATAATACTACTAGTAATAACGTGAGTAATGAAAAGTGTTGTAAATTTTTTTTGAAATTCATCGGTACAAGAGAATTACTGTTCTAATGGATTTTTTTTTCTTTA
TARGET=6,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00299 Ps_contig_mira-and-tgicl-ass_franssen_3323
SEQUENCE=TAWGCATGATGATGATGATGATGAGAGAGAGAGAGAGGTTGAAGGTGGATGGCATGGTGTGGGGTCGGAGATGAAAGGTTGATGATGATTAAGACCGTGCGGGAAAGGGTTAATTAATGAATAATACTACTAGTAATAACGTGAGTAATGAAAAGTGTTGTAAATTTTTTTTGAAATTCATCGGTACAAGAGAATTACTGTTCTAATGGATTTTTTTTTCTTTA
TARGET=23,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00300 Ps_contig_mira-and-tgicl-ass_franssen_3324
SEQUENCE=TTTTAMGWAAAAAAATACCATTAGAACAGTAATTCTCTTGTACCGATGAATTTACAAAAAAAATTTACAACACTTTTCATTACTCACGTTATTACTAGTAGTATTATTCAATTTAACTTTAACCCCTTTCCCGCACGGTTCTTAATCATCATCAACCTTTCATCCTCCCGACCCCACACCATACCATCCACCTTCACCTCTCTCTCTCTCNCATCATCATCATCATCATGTCTTAC
TARGET=198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00301 Ps_contig_mira-and-tgicl-ass_franssen_3324
SEQUENCE=TTTTAMGWAAAAAAATACCATTAGAACAGTAATTCTCTTGTACCGATGAATTTACAAAAAAAATTTACAACACTTTTCATTACTCACGTTATTACTAGTAGTATTATTCAATTTAACTTTAACCCCTTTCCCGCACGGTTCTTAATCATCATCAACCTTTCATCCTCCCGACCCCACACCATACCATCCACCTTCACCTCTCTCTCTCTCNCATCATCATCATCATCATGTCTTAC
TARGET=212,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00302 Ps_contig_mira-and-tgicl-ass_franssen_3325
SEQUENCE=AGTGGTATCAACGCAGAGTGGAATTAACTAGGGAGGAAGAAATGGGAATCGGGAGTTTGATTTGAGAGGTGCGAATAGAAAAAAAAATAAGACATTATGCGCAACGGTGTATAGATGAAAATGGATAGATGAAGAAACTGCATGATGATGATGATGATGAGAGAGAGAGAGGTAGGTTAGGAAGGTGGAGTGGCTAGTTGGGTGGGTGGGGGTACGGAARTAAGKTAAGGGTAAAAGGGTTTGAAGTAGAAGTTAGGTAAAACGTTTAAACCGGACCCGGGTAGGGGTAAAACGGGGGTTTAAAAAGGTTTTTAAAA
TARGET=142,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00303 Ps_contig_mira-and-tgicl-ass_franssen_3325
SEQUENCE=AGTGGTATCAACGCAGAGTGGAATTAACTAGGGAGGAAGAAATGGGAATCGGGAGTTTGATTTGAGAGGTGCGAATAGAAAAAAAAATAAGACATTATGCGCAACGGTGTATAGATGAAAATGGATAGATGAAGAAACTGCATGATGATGATGATGATGAGAGAGAGAGAGGTAGGTTAGGAAGGTGGAGTGGCTAGTTGGGTGGGTGGGGGTACGGAARTAAGKTAAGGGTAAAAGGGTTTGAAGTAGAAGTTAGGTAAAACGTTTAAACCGGACCCGGGTAGGGGTAAAACGGGGGTTTAAAAAGGTTTTTAAAA
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00304 Ps_contig_mira-and-tgicl-ass_franssen_3325
SEQUENCE=AGTGGTATCAACGCAGAGTGGAATTAACTAGGGAGGAAGAAATGGGAATCGGGAGTTTGATTTGAGAGGTGCGAATAGAAAAAAAAATAAGACATTATGCGCAACGGTGTATAGATGAAAATGGATAGATGAAGAAACTGCATGATGATGATGATGATGAGAGAGAGAGAGGTAGGTTAGGAAGGTGGAGTGGCTAGTTGGGTGGGTGGGGGTACGGAARTAAGKTAAGGGTAAAAGGGTTTGAAGTAGAAGTTAGGTAAAACGTTTAAACCGGACCCGGGTAGGGGTAAAACGGGGGTTTAAAAAGGTTTTTAAAA
TARGET=199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00305 Ps_contig_mira-and-tgicl-ass_franssen_3326
SEQUENCE=CCCCTAACCGGGTCCGTTTAACTTTACCGTAATCCAATCAAACCTTTACCCTACTTACTTCCGTACCCCCGAACCCAACCCAACCTAGTTCATCCACCTTCCTAACCTCTCTCTCTCTCATCATCATCATCATCATCATGCAGTTTCTTCATCTATCCATTTTCATCTATACACCGTTGCGCATAATGTCTTATTTTTTTTTCTTATTTCGCACCTCTCAAATCAAACTCCCGATTCCCATTTCTTCCTCCCTGTTAATTCCACTCTTCCGAACAATACCATTGAAGCGTCGGCGGTTGTTCGTACGGAAAAGAAATTACAAGTCTCATCGGCAACTAAAGAAAATTNTGCTCTTCCATTTTCACATACTGTGTCTGATACTCATTCTAGAAACTAGAAAAACATTACTCATCGAAGACGGCGAAAGAAGATAGAAAACCGAAACCCCCCCCTAAAATTACG
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1563
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.020269
PRIMER_LEFT_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT=79,22
PRIMER_RIGHT=180,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.020
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=0.1563
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.020269
PRIMER_LEFT_1_SEQUENCE=CAACCTAGTTCATCCACCTTCC
PRIMER_RIGHT_1_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=180,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.5424
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.406341
PRIMER_LEFT_2_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_2_SEQUENCE=CATTATGCGCAACGGTGTATAG
PRIMER_LEFT_2=79,22
PRIMER_RIGHT_2=187,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.406
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=4.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=0.5429
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.406861
PRIMER_LEFT_3_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_3_SEQUENCE=AATCGGGAGTTTGATTTGAGAG
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=235,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.593
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.5429
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.406861
PRIMER_LEFT_4_SEQUENCE=CAACCTAGTTCATCCACCTTCC
PRIMER_RIGHT_4_SEQUENCE=AATCGGGAGTTTGATTTGAGAG
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=59.593
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=156
=
PRIMER_SEQUENCE_ID=SSR_00306 Ps_contig_mira-and-tgicl-ass_franssen_3326
SEQUENCE=CCCCTAACCGGGTCCGTTTAACTTTACCGTAATCCAATCAAACCTTTACCCTACTTACTTCCGTACCCCCGAACCCAACCCAACCTAGTTCATCCACCTTCCTAACCTCTCTCTCTCTCATCATCATCATCATCATCATGCAGTTTCTTCATCTATCCATTTTCATCTATACACCGTTGCGCATAATGTCTTATTTTTTTTTCTTATTTCGCACCTCTCAAATCAAACTCCCGATTCCCATTTCTTCCTCCCTGTTAATTCCACTCTTCCGAACAATACCATTGAAGCGTCGGCGGTTGTTCGTACGGAAAAGAAATTACAAGTCTCATCGGCAACTAAAGAAAATTNTGCTCTTCCATTTTCACATACTGTGTCTGATACTCATTCTAGAAACTAGAAAAACATTACTCATCGAAGACGGCGAAAGAAGATAGAAAACCGAAACCCCCCCCTAAAATTACG
TARGET=118,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1563
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.020269
PRIMER_LEFT_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT=79,22
PRIMER_RIGHT=180,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.020
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=0.1563
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.020269
PRIMER_LEFT_1_SEQUENCE=CAACCTAGTTCATCCACCTTCC
PRIMER_RIGHT_1_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=180,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.5424
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.406341
PRIMER_LEFT_2_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_2_SEQUENCE=CATTATGCGCAACGGTGTATAG
PRIMER_LEFT_2=79,22
PRIMER_RIGHT_2=187,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.406
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=4.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=0.5429
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.406861
PRIMER_LEFT_3_SEQUENCE=CCAACCTAGTTCATCCACCTTC
PRIMER_RIGHT_3_SEQUENCE=AATCGGGAGTTTGATTTGAGAG
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=235,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.593
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.5429
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.406861
PRIMER_LEFT_4_SEQUENCE=CAACCTAGTTCATCCACCTTCC
PRIMER_RIGHT_4_SEQUENCE=AATCGGGAGTTTGATTTGAGAG
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=59.593
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=156
=
PRIMER_SEQUENCE_ID=SSR_00307 Ps_contig_mira-and-tgicl-ass_franssen_3330
SEQUENCE=TTACKGAGTTAGTTATCATCGGCAAAAAACTGATTGCAAGAAAGAGAAAATCCAAAATCCAAAATCCAAACTCATAGAACTAGTAATGTCTCTGAACGCTCTACTTCCACCTCCTCTATCGAGTTCCTCTTCAAGCTTACGCTCCTCCTCCTCCTTCAACCACTCTCTCTTTTCCATTCCAAAACAAATCCCCAAAAGAACTCACCACCACCACCACCACCATGTGTTGGTGGTTGAAGCAAAGGGTAAAAAAGGAATGATGTCCGGCCAATTCCAGCGCCCTCCCCCTCCTCCTCCTTTACCCAAAATCGAAGACGACGGTAACCCCAAATTCGTCGTCTTCATTCGCATGGCTAATATTTACCTTTGGTATCCTCTTAGTATCGTTTCCGGTGGCACCACCGCCAAAATCATGGTTGCCGCTAAAGATAACTTTCTCGGCAAATATATCTACAAGGATACTCTCGCTAGAAATCTCGCTTCTGTTATCTACCAAGATGAGA
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00308 Ps_contig_mira-and-tgicl-ass_franssen_3330
SEQUENCE=TTACKGAGTTAGTTATCATCGGCAAAAAACTGATTGCAAGAAAGAGAAAATCCAAAATCCAAAATCCAAACTCATAGAACTAGTAATGTCTCTGAACGCTCTACTTCCACCTCCTCTATCGAGTTCCTCTTCAAGCTTACGCTCCTCCTCCTCCTTCAACCACTCTCTCTTTTCCATTCCAAAACAAATCCCCAAAAGAACTCACCACCACCACCACCACCATGTGTTGGTGGTTGAAGCAAAGGGTAAAAAAGGAATGATGTCCGGCCAATTCCAGCGCCCTCCCCCTCCTCCTCCTTTACCCAAAATCGAAGACGACGGTAACCCCAAATTCGTCGTCTTCATTCGCATGGCTAATATTTACCTTTGGTATCCTCTTAGTATCGTTTCCGGTGGCACCACCGCCAAAATCATGGTTGCCGCTAAAGATAACTTTCTCGGCAAATATATCTACAAGGATACTCTCGCTAGAAATCTCGCTTCTGTTATCTACCAAGATGAGA
TARGET=203,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00309 Ps_contig_mira-and-tgicl-ass_franssen_3330
SEQUENCE=TTACKGAGTTAGTTATCATCGGCAAAAAACTGATTGCAAGAAAGAGAAAATCCAAAATCCAAAATCCAAACTCATAGAACTAGTAATGTCTCTGAACGCTCTACTTCCACCTCCTCTATCGAGTTCCTCTTCAAGCTTACGCTCCTCCTCCTCCTTCAACCACTCTCTCTTTTCCATTCCAAAACAAATCCCCAAAAGAACTCACCACCACCACCACCACCATGTGTTGGTGGTTGAAGCAAAGGGTAAAAAAGGAATGATGTCCGGCCAATTCCAGCGCCCTCCCCCTCCTCCTCCTTTACCCAAAATCGAAGACGACGGTAACCCCAAATTCGTCGTCTTCATTCGCATGGCTAATATTTACCTTTGGTATCCTCTTAGTATCGTTTCCGGTGGCACCACCGCCAAAATCATGGTTGCCGCTAAAGATAACTTTCTCGGCAAATATATCTACAAGGATACTCTCGCTAGAAATCTCGCTTCTGTTATCTACCAAGATGAGA
TARGET=287,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00310 Ps_contig_mira-and-tgicl-ass_franssen_3331
SEQUENCE=CGAACATCTTGTWTGCAAGAATAAGAACTGTTACTAACATAGAAAAATCAGCATAACTAGCATCAAATAACAAGCTAAAGAAGAATCACAACTAAATCAAACCCAGCRTAAAAAACACTATTATCATTGCCGTTTTCAGGATACAACATAGATAATCCCCTCAAATGTTTGAACCAAATTACACTGGATATCAGATTTAAGTTATTTGAAAGTTTAACCAAAACTAACATTTACCGAAGAACACAAACCCTAATCAATCAATCGTGCAGAATACCCAAAATGTCCTCGTCCCTAAACAAATGGAACTCTTTGTCATCAAGCTTAATTTGAGAGCCACCGTACTCCGGCAAGAGAACGTGGTCACCTTC
TARGET=252,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00311 Ps_contig_mira-and-tgicl-ass_franssen_3355
SEQUENCE=AAGCAGGTGGTATACACGCAGAGTACGSGGACACCATCACCACATTCGTCTTCTCTTTCTCTCTCTCTCAATTCCTCCTTTTCAGGAATTTGGAGAGAGTAGTGATTAACCAAACATGAAGTTTTCAATGTATTTTTCTTGTTAGCAACATTCTATTGCTTCTAAGTTCTTCACCAGCTATTTYGACCAAAACTGTAACGTCCACCGGCACCGATCGCGCCGACACCGCTCCAACTCCCGGCCCCAGCCACCTGCCAACCAGTA
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00312 Ps_contig_mira-and-tgicl-ass_franssen_3357
SEQUENCE=TTACCAACGGACGTGTGGCGGCCCGACTGCCTCTCAAAAACTTAACTTCTATTACTTGAAAATAGCTTTCTTCCTTAATTGTCATTGTTCTTTAGTCAAAATCACGTGTCCCCTTAGGAATCTTGGGCAATCTTCTGCTTTCATTGCTTTCTACAACATCCTTTACTTTCAATAAAAATTCATTGAATTCAGGTCTTGCCAAATCCAAAAACAATAGAAAATCAGCAACCCAAGTCTCTACATCACTCTTTTTTTCACTCTGCAACCTATCCTTAATCAACTGCAAAATCGAACTAGTTCCTTTTCCCAAAACAGCCTTCTTCTTCTTCTTTTCTACTTTGGAATTCTCTCCATTTTCTGTAGCACCTCGGATCTTTTCATTCAACTCACCAGCCTCAAGTACAGCATAAGCAGTCACAAGTTCCCACGCCACAATTTTCCACACAAGCGCAAACAACACATTGATTTCATGTGCAAACTTATCATACACCTTTCCAGAATACAAATTGAAAGACTCGTTAAAACTACTAAGCAACCTAGCATCAGTTGGACATTCCTTCCCAAAGATTTCACCAATTTTCGATTTCAAATCATCACCACCAATAGCCAACAAGTTACTTTGTGCACGAGCAAAACTACAGTCACCCAATTCCCTCAATGCCATTGCCAAAGGCAACAACACATTGCACACAGTATGCTCACTCCCAAGCTCAATTTTCCCCAATTTATGACCGGAATTCAACTCAACTCGCATCCTAGAATGCACTGATTTCGCCTTCTCCCTAATAATCCCATGAATTTTAGGAATTTTCACAACCGAAGAAACAGTTTCCTTTCCCACAAACTTAGATCCATTAAGCAAAATCCTCATATCACCAGCAACTCCAACTTCCTCCTTAGAACTATGTCCATCACCGGAATCCATCAAATTGAAAACACTCACATCAGCCTTCAAAGCCTCACAAGAAAACGCCGCAGCCACATCAGCAAAAGCAGACAGCGCCGTCGATTGATGATCCAAAACTGCGGAAACACCAATCAACCCTAAACACCCATTATTGATCAACAATGACTGTTCGTCTTCAGTCACATCGAGTTCTTCTTCGAAATGAAAACTCTTCTGATTCAAATTCAGTGTTTCTGATATGCATAATGGAACGTGTTTAGATGAGTTGCAGAGGAGGAGTTTGTTGAGAAGGACGATGGAGGAAGGCTCTTGTTTCTTCGTAGGGTTAGGTAA
TARGET=317,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2120
PRIMER_LEFT_PENALTY=0.116582
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=GAAAATCAGCAACCCAAGTCTC
PRIMER_RIGHT_SEQUENCE=AATGAAAAGATCCGAGGTGCTA
PRIMER_LEFT=216,22
PRIMER_RIGHT=381,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.3786
PRIMER_LEFT_1_PENALTY=0.116582
PRIMER_RIGHT_1_PENALTY=0.261998
PRIMER_LEFT_1_SEQUENCE=GAAAATCAGCAACCCAAGTCTC
PRIMER_RIGHT_1_SEQUENCE=AGATCCGAGGTGCTACAGAAAA
PRIMER_LEFT_1=216,22
PRIMER_RIGHT_1=374,22
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=60.262
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.3786
PRIMER_LEFT_2_PENALTY=0.116582
PRIMER_RIGHT_2_PENALTY=0.261998
PRIMER_LEFT_2_SEQUENCE=GAAAATCAGCAACCCAAGTCTC
PRIMER_RIGHT_2_SEQUENCE=AAGATCCGAGGTGCTACAGAAA
PRIMER_LEFT_2=216,22
PRIMER_RIGHT_2=375,22
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=60.262
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=0.3786
PRIMER_LEFT_3_PENALTY=0.116582
PRIMER_RIGHT_3_PENALTY=0.261998
PRIMER_LEFT_3_SEQUENCE=GAAAATCAGCAACCCAAGTCTC
PRIMER_RIGHT_3_SEQUENCE=AAAGATCCGAGGTGCTACAGAA
PRIMER_LEFT_3=216,22
PRIMER_RIGHT_3=376,22
PRIMER_LEFT_3_TM=60.117
PRIMER_RIGHT_3_TM=60.262
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=0.3786
PRIMER_LEFT_4_PENALTY=0.116582
PRIMER_RIGHT_4_PENALTY=0.261998
PRIMER_LEFT_4_SEQUENCE=GAAAATCAGCAACCCAAGTCTC
PRIMER_RIGHT_4_SEQUENCE=AAAAGATCCGAGGTGCTACAGA
PRIMER_LEFT_4=216,22
PRIMER_RIGHT_4=377,22
PRIMER_LEFT_4_TM=60.117
PRIMER_RIGHT_4_TM=60.262
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_00313 Ps_contig_mira-and-tgicl-ass_franssen_3358
SEQUENCE=TTCGGGTATACCAGATAATCTCCGTTGGAGGGCATTACAGTAAATGCAAGAATTATTGCCGCCGCCTTCGTCATTACCATCCCGGTGGGCCCGTTTCGCGATCTTCAAATTTTTGGAAGTTCCTTATAAGAAAACGCTTCCAAATAAKKSRSGACCCTTGATTTTGAATTCACGATAGATAAACAAGATAAGGTGTTTTTGTTTTTGCGTGTGTGTGTATGCGAAGGAAGAAAGAGTTTTTCGAGCAATGGGAAGAGCGTTCGTGTACGTGATTCTCGGTGGTGGTGTTTGAGGCATTTTATAAGAAAGATCTTGCTAAAAGACTACTGTTAGGAAAGAGTGCTTCGATTGATGCAGAGAAATCCATGATCTCCAAGTTGAAGACTGAGTGT
TARGET=209,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00314 Ps_contig_mira-and-tgicl-ass_franssen_3361
SEQUENCE=AGTACGCGGGACCGTCCCTTTCCCCTTTTCSAATGATCTCAATTCATAACTCTTCTTTCTTTCCCCACCTATGCATTCATCACATTTTCCCCAAATTTCTCAATCTCTTCCTCTTCCCAACCCTAAATTCTCCATCCTTTCAAAACCCTAATCCAAACCCAAACCCTCTTAAGGGCTTTTAAACCTTTTCCTTAAATTCATCATTTACTCACTCAGATAACAATTCAAACCAAAAGCTCAAACCTTTCCCATCCCAGATTGCTTTTTTCATCCACTTCATTTTCAATATGCACGCAAAAACTGATTCTGATGTTGCAAGTTTCGACCCATCGTCACCCAGTTCACCAAAACGAACCGTTTACTATGTTCACAGTCCTTCTAGAGACTCTCATGATGGTGACAAATCTTCAACTATGCAACCAAGTCCTATGGATTCACCTTCACATCAATCCTATGTTCACCATTCAAGAGCTTCTTCTTCCAGCAGAATCTCTGGTGGTTATAACTCTTCTTTGGGTAAGAAAGGGAATAGAAAAGGTAATGATAAAGGCTGGCCTCATTCTAAGGTTATAGAAGAAGAAGATGGTGATTTTTATCATGAGAGTAATGGAATTTCTAGGAGGTTTCAAATCTTCATTGCTATTGTCGGGTTTGTGCTGGTTTTGCYGTCTTTTG
TARGET=572,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00315 Ps_contig_mira-and-tgicl-ass_franssen_3363
SEQUENCE=TAACTTTCTCATTAGGTCATGCTGATGACAAATGCAAAGAGCCTCTTGCTGTTATGGCCTTTGCTGGTGACCAAACTCAGAACATTCATTAAACAAACAAACCAAAATTACGAATTCTACTTTTCAAGTTTCTAATCTGAATTTCACTGCCAAAGCTGATATGACTAGAATTGCCTTCTATAGTGTTTACTACAATACTAGAAGTGATGATATGAGCTCTCTTTGTGGGCCTGTTGTGGATGATGTGGAGGGTTTGGTTTTCTAGGTCTAACGGGCTTCGTGGGCTGGGCTTTGAAGACTTGGGCTTGGGTATTTTKGGKTTTAGTTTTGGTTCTTTAGGTTTWAAARGGGGTTNATTTATTGGTTTTTGGTTTATTTAATGTTTATTACTACTAAGCTTGATTAGGTTTCTTTTGTAGTTTTGGGGG
TARGET=91,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00316 Ps_contig_mira-and-tgicl-ass_franssen_3404
SEQUENCE=TAGCTTAGGTTTTTTCCTCTGTTGGTAAACCAGCTCCATYAGCACTCTCACACTCGATCTCAYACAAAGTTCCAAAAATCAAAATCAGTCTCATCCACTTCCAATTTCAACCCCTTTCCAATCATWAAMATTCCTCACATTCCGAAAACCTACCTAAACCTAAAAACCCAATTTTCTACCCACCCACCCCAAACTTCCTCCTTCACCCTTTTCTAGAATCCTCGACTCCAACAAACCCACGCTTCCCCGGTTCCGAGAACACATTCAC
TARGET=178,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00317 Ps_contig_mira-and-tgicl-ass_franssen_3418
SEQUENCE=AAGGCAAAGACACGCTCGTCTTTCACTGTTCGTCTTTCTTTAAGTTTCTTAGGTTTTCGTTAGGTTCCCTTAAGTTTTGTTGTCAAGGAAGCTTGTCAATTATCTTGAAGAGAACAAGGAAGATGTTGGGATAAAGTTAACTATTATGGTGTTGGAACGTGGCTTCAATGGGTGGGAAGCTTCTGGTAGACCTGTTTGCCGCTGCAACAATGTTCCTTGTAAGGAGCATGAGAATGCTTGATCAAAGGCTTATTCTGGTANACCCATCTTACCTGTACTATCTGCAAACTNTTTGATAACTAAAGAAATGTATGAACTTATTAATTAATTACATCATTATAATAATCATAGGTAAGCTAG
TARGET=320,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00318 Ps_contig_mira-and-tgicl-ass_franssen_3481
SEQUENCE=AAAAGTTTCAACATCCAAGAGATATAATTCAGACCAACCAGTTACATTATGTAATCTGCAGCCCATAAACTTGTTGGAGCTTCGCATAAAGACTCGAAGTGCGTATAGTATACACAGAAACACAGAAAACAAGTTTAACTAAGATGGAACAAACAGAATAAATAATTATGAACAACATGATGCAGCTCTGGATCCTTCGGCGGGTCTATCAACAAGAACCATTCCTGCTGGTTGTGCTGGCTGTGCCCTCGGTAACCTCTTGGCTATTTCATAAAATATTTCATTGACATTGGCTGCAGTTTTTGCAGAGGCCTCAATGAAAAACAGACCATTTTCCTCAGCATATACACGTGCTTCTTCAGCTGTCACTTTCCTTTTATCTTCCAAGTCAGCTTTGTTACCGGCAAAAGCCACGACCATGTTAGGATTCCCTTGTTTCTGAAGCTCTTGGACCCATTTCTTCGCCCGTGTAAATGAATCCAAGTTAGTAATGTCATAGACAATGATAGCAGCAGCTGCGCCTCTATAGTACATGGGGGCCAAGCTATGGTATCTCTCTTGTCCTGCTGTGTCCCAAATCTCAAATTTCACTGTGGCGTCATTTACTGCCAGCGTCTGGGAGAAAAATGCTGCCCCTATTGTTGATTCCTGAAACTCAAGGAATTGACCCTTGACAAAACGCAATACAAGGCTTGATTTTCCAGCACCCATATCCCCAAGCAGAACCAATTTGGCGTTGAGGTTGCTGTGTCCGATCGTAGCCATTGAAGTGAAATTGGAGCGATCGGAATGGAAGAAGCTTCGAGACGAAGAAATTGAAATTAAAAAAAAAGAGGAAGAAGAAGAAGACGCCGAAAACAGAGTCCTTCTCCTAGTTGTATTGTTATAGCTTTTAGCTTTGCGTGCAAACGCGCTTTTCTTTTCTTTTACTACTATTTTCTAATGTTTTTTGTTCWSMGACAGATTCCACTCAGCTTTCC
TARGET=836,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00319 Ps_contig_mira-and-tgicl-ass_franssen_3481
SEQUENCE=AAAAGTTTCAACATCCAAGAGATATAATTCAGACCAACCAGTTACATTATGTAATCTGCAGCCCATAAACTTGTTGGAGCTTCGCATAAAGACTCGAAGTGCGTATAGTATACACAGAAACACAGAAAACAAGTTTAACTAAGATGGAACAAACAGAATAAATAATTATGAACAACATGATGCAGCTCTGGATCCTTCGGCGGGTCTATCAACAAGAACCATTCCTGCTGGTTGTGCTGGCTGTGCCCTCGGTAACCTCTTGGCTATTTCATAAAATATTTCATTGACATTGGCTGCAGTTTTTGCAGAGGCCTCAATGAAAAACAGACCATTTTCCTCAGCATATACACGTGCTTCTTCAGCTGTCACTTTCCTTTTATCTTCCAAGTCAGCTTTGTTACCGGCAAAAGCCACGACCATGTTAGGATTCCCTTGTTTCTGAAGCTCTTGGACCCATTTCTTCGCCCGTGTAAATGAATCCAAGTTAGTAATGTCATAGACAATGATAGCAGCAGCTGCGCCTCTATAGTACATGGGGGCCAAGCTATGGTATCTCTCTTGTCCTGCTGTGTCCCAAATCTCAAATTTCACTGTGGCGTCATTTACTGCCAGCGTCTGGGAGAAAAATGCTGCCCCTATTGTTGATTCCTGAAACTCAAGGAATTGACCCTTGACAAAACGCAATACAAGGCTTGATTTTCCAGCACCCATATCCCCAAGCAGAACCAATTTGGCGTTGAGGTTGCTGTGTCCGATCGTAGCCATTGAAGTGAAATTGGAGCGATCGGAATGGAAGAAGCTTCGAGACGAAGAAATTGAAATTAAAAAAAAAGAGGAAGAAGAAGAAGACGCCGAAAACAGAGTCCTTCTCCTAGTTGTATTGTTATAGCTTTTAGCTTTGCGTGCAAACGCGCTTTTCTTTTCTTTTACTACTATTTTCTAATGTTTTTTGTTCWSMGACAGATTCCACTCAGCTTTCC
TARGET=914,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00320 Ps_contig_mira-and-tgicl-ass_franssen_3532
SEQUENCE=TAAAGGTTAACATCCTAATGTTGCTGAGATGAGAAGGATATATATAATGTAATGTAATGATTAAACTCTCTAAACTTTTGTATTAACTTTTTTATTCTGTCGGTTTTGTTTTGAGCACTTAACAAGAGAAGTGTCTTTTGTGACTTCTTATTTTCTATCATCACTATATTCATGTACTAAAATTTAAAAGT
TARGET=45,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6163
PRIMER_LEFT_PENALTY=1.063505
PRIMER_RIGHT_PENALTY=0.552823
PRIMER_LEFT_SEQUENCE=AATGTTGCTGAGATGAGAAGGA
PRIMER_RIGHT_SEQUENCE=AAGTGCTCAAAACAAAACCGAC
PRIMER_LEFT=16,22
PRIMER_RIGHT=119,22
PRIMER_LEFT_TM=58.936
PRIMER_RIGHT_TM=60.553
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=2.3405
PRIMER_LEFT_1_PENALTY=1.063505
PRIMER_RIGHT_1_PENALTY=1.277028
PRIMER_LEFT_1_SEQUENCE=AATGTTGCTGAGATGAGAAGGA
PRIMER_RIGHT_1_SEQUENCE=GCTCAAAACAAAACCGACAGA
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=115,21
PRIMER_LEFT_1_TM=58.936
PRIMER_RIGHT_1_TM=60.277
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=2.5766
PRIMER_LEFT_2_PENALTY=1.063505
PRIMER_RIGHT_2_PENALTY=1.513104
PRIMER_LEFT_2_SEQUENCE=AATGTTGCTGAGATGAGAAGGA
PRIMER_RIGHT_2_SEQUENCE=GCTCAAAACAAAACCGACAGAA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=115,22
PRIMER_LEFT_2_TM=58.936
PRIMER_RIGHT_2_TM=61.513
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=100
PRIMER_PAIR_PENALTY_3=2.6399
PRIMER_LEFT_3_PENALTY=2.087031
PRIMER_RIGHT_3_PENALTY=0.552823
PRIMER_LEFT_3_SEQUENCE=CTAATGTTGCTGAGATGAGAAGGA
PRIMER_RIGHT_3_SEQUENCE=AAGTGCTCAAAACAAAACCGAC
PRIMER_LEFT_3=14,24
PRIMER_RIGHT_3=119,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=60.553
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=2.6399
PRIMER_LEFT_4_PENALTY=2.087031
PRIMER_RIGHT_4_PENALTY=0.552823
PRIMER_LEFT_4_SEQUENCE=TCCTAATGTTGCTGAGATGAGAAG
PRIMER_RIGHT_4_SEQUENCE=AAGTGCTCAAAACAAAACCGAC
PRIMER_LEFT_4=12,24
PRIMER_RIGHT_4=119,22
PRIMER_LEFT_4_TM=59.913
PRIMER_RIGHT_4_TM=60.553
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_00321 Ps_contig_mira-and-tgicl-ass_franssen_3535
SEQUENCE=TTTCACGRCCCAAAGTCACTGTCTTGATTGACATTAAGAAACACCAAACAAACCATCTCAATAAACTAAGCTACCACTAMTACTAATATTAAATAAATAAA
TARGET=90,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_00322 Ps_contig_mira-and-tgicl-ass_franssen_3554
SEQUENCE=TAAAATTAACTTAGTCATCATAAAAATTACTAGYCTTAAAATTACTTGCATCAAGTACAACCAAAATTACTGCNTTAAAAGGACATGTAGAAATTACTGCATAAAATGCATCAAAATTCTGCTGCCAAGCCAAACAAGCCACAAGCGAGCCAAGCCGGTAAACCGCAAGCAAAACGCAGCTAATACACAAACCTTCTAACAAAACCTGCATAAAAGAAAGCAAAAGCATAACAGTGGAAAGGAGGCAAAATAGAAGCATAGCATAATAGAGAATTGGAAACACTCACTGAAACCATGACTAACTTCGTGTGAGAACCGACTCGGTAACTAACCTGCCAAGTGCCATAACCGCCTCATAACAGAATAACTAACTCCACAAGATAGAAATCCAACTTCAAAACACCTTCAGAACCTATTTTCCTCATCCTCCTTCAACTCTCATAACAACTTCCAACTGTCATAACCAACCATCTCTCTAACCTTACTCTCCTATAAGAACCATCCTCCATCTTCAATTCAAGTTTCTTCCATCATCTTTCCCATAATTCAACAATTCACCATTCTTCTCCACCTCCACTCTCAATCCACTTCCAGCTTCTTCGTCAAAACCTTCACCAAACCCCAAATTCCATAAACATAATCTTCTTCAACATCAACCTTCACCTTCCAATCCACACCTTCACCACTACGGATCCGTAACCGTAATCTCCACCTTCACAAGATCTTCAACAACCTCTCTACAATTTTTCCGGGATTCTCCTTTTCTTCCTCCATCGCCTATTCAATTCTTCAACCTCATCACAAACTCTTCGAATCATTGAACCCAAACCTTCAACCACCGTGAATCCATCCAAGAACTCCTCTTCATCATAACTGCAACCAACCAAAACCGTAACCGAAACCGTAATCTCCACCTTCATCAAACCTTCACCAATTCGCAACGGTGCGGTCACGACGAAATCGCGGTCAAAAATCAAACGCCTTCATCTCCGTTACGGTCAACGACGCGCCACGACGAACAACAACAACACGGCTCGAACACGCGGGTTCGTGTGATTCTTCTGGTGACGAAGGAGGATACGAAGAAAGAGAAGTAGAAAGTGGAACACATCGCTTGATTACGGATCTGATCAAGGTGGACGTCAGCTTCACTCCGACGACGTGTTGGTAACGACATTACGCAAACCGTTCCACTCTGGCGAAGACGCATCGACGGCGATGATGCACGGTTGAGGCGCGAGCAGTTTTACTCCGCCGGTAGCGTGTTGACGACGATGATGTGCGGTTGTTACACCAGCAGTTTCCGTTCATGGACGTTTTGACTCCTGAAGGTGAGTTTCGGTTTTCTCATCTGAACCTGTCTTTAGGGATTTCATAGAACATTTTCAATTACCCCGT
TARGET=1018,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00323 Ps_contig_mira-and-tgicl-ass_franssen_3587
SEQUENCE=CCCTTTCAAGTGTTGAATTCCAACCACAAATGTGTTAAGAAATCCTCCAATTGAAGRATGTGATAATATCTTACCACTTGTGGAGCCCAACCTCTAATTATAAGGCCTCTCTCTTTGTTCCTTTCCTCAACCTTTCTTCACTAATCCATTTTTCCAACTCTTCACTTTTATTTCCTTCTCTAATAACCCAAATAAATGGCCTTTGTGTTGCTTCCAAGGCTAAAGCCAATTCCATAAGCTGTGAAGGAACTAAGTTGCATAAACTTCCAAGACAAGCATAGATCACAGACTTTGGTTGGTGCAAATCTAGCCAATTTAAGCAATGATGTTGACTTATTGAAGCCATGTTACCTCTTTGTGCTTTGTCCAACCCATCTTTGTTGCAAAGGGAAACAGGTCCAACACACCACACTTTATCATTTCTTTCTTTCTTGTAATCCTTAACATATGCTTCCTCCAACTCCTCAAAAGTGTTTATGATTTCACCATATGACCTTACTTCAGCCTCATTCATCTTCTCTTTAAACTCCTTAAACTCATCTGGTATTGTTCCTCCTGGTACTTGCACTTTGGTGACATGAATTTGGTCAGGAATACCAGGCACAGTGAAATACTCAGATTCTGAATCGATACTTTCAAGAATCTTCGATGCATTTACCTTGACCCGCGTACTCTGCGTTGA
TARGET=420,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00324 Ps_contig_mira-and-tgicl-ass_franssen_3594
SEQUENCE=GATTCAGACTTTGATTTTTTTCCCGAAAATAATAAAATAAAATAAAAAATAGCCCTGTGTTTTTCCATTTCCTCTAAGAGAGTGAGTTAATGGCAGAAGAAGAAGAAGAATCTGAACTCAGCGAGAAGCAAAAAGTTGAAATCGCGAAATGGTTTCTTCTCAACTCTCCTCCCGGAGAGATACGAAATCCGAAAGAGATAAAGAACATGCTTGACTTTCATCCCCAACGGATTGGACATGCTTGTTTCTTCGG
TARGET=31,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00325 Ps_contig_mira-and-tgicl-ass_franssen_3594
SEQUENCE=GATTCAGACTTTGATTTTTTTCCCGAAAATAATAAAATAAAATAAAAAATAGCCCTGTGTTTTTCCATTTCCTCTAAGAGAGTGAGTTAATGGCAGAAGAAGAAGAAGAATCTGAACTCAGCGAGAAGCAAAAAGTTGAAATCGCGAAATGGTTTCTTCTCAACTCTCCTCCCGGAGAGATACGAAATCCGAAAGAGATAAAGAACATGCTTGACTTTCATCCCCAACGGATTGGACATGCTTGTTTCTTCGG
TARGET=95,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5760
PRIMER_LEFT_PENALTY=0.343977
PRIMER_RIGHT_PENALTY=0.232060
PRIMER_LEFT_SEQUENCE=TAGCCCTGTGTTTTTCCATTTC
PRIMER_RIGHT_SEQUENCE=AGTTGAGAAGAAACCATTTCGC
PRIMER_LEFT=49,22
PRIMER_RIGHT=164,22
PRIMER_LEFT_TM=60.344
PRIMER_RIGHT_TM=59.768
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=0.7043
PRIMER_LEFT_1_PENALTY=0.343977
PRIMER_RIGHT_1_PENALTY=0.360320
PRIMER_LEFT_1_SEQUENCE=TAGCCCTGTGTTTTTCCATTTC
PRIMER_RIGHT_1_SEQUENCE=AAGAAACAAGCATGTCCAATCC
PRIMER_LEFT_1=49,22
PRIMER_RIGHT_1=249,22
PRIMER_LEFT_1_TM=60.344
PRIMER_RIGHT_1_TM=60.360
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.7326
PRIMER_LEFT_2_PENALTY=0.500551
PRIMER_RIGHT_2_PENALTY=0.232060
PRIMER_LEFT_2_SEQUENCE=CCTGTGTTTTTCCATTTCCTCT
PRIMER_RIGHT_2_SEQUENCE=AGTTGAGAAGAAACCATTTCGC
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=164,22
PRIMER_LEFT_2_TM=59.499
PRIMER_RIGHT_2_TM=59.768
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=0.8609
PRIMER_LEFT_3_PENALTY=0.500551
PRIMER_RIGHT_3_PENALTY=0.360320
PRIMER_LEFT_3_SEQUENCE=CCTGTGTTTTTCCATTTCCTCT
PRIMER_RIGHT_3_SEQUENCE=AAGAAACAAGCATGTCCAATCC
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=59.499
PRIMER_RIGHT_3_TM=60.360
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=1.1206
PRIMER_LEFT_4_PENALTY=0.343977
PRIMER_RIGHT_4_PENALTY=0.776671
PRIMER_LEFT_4_SEQUENCE=TAGCCCTGTGTTTTTCCATTTC
PRIMER_RIGHT_4_SEQUENCE=CTTTCGGATTTCGTATCTCTCC
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=194,22
PRIMER_LEFT_4_TM=60.344
PRIMER_RIGHT_4_TM=59.223
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_00326 Ps_contig_mira-and-tgicl-ass_franssen_3595
SEQUENCE=GAAGTACGCCGGGGGGGAGGGTAAACCGWAACCTTTWCTAAGTTCAATCTGTTTTACTATTTGTAAGCTGAAAGAGAAAGTTCGTGTTGTGCATAGTTTCAAGAAAGTTAAAACTGATGATGATGAAGCATGGCAAGTGTTGAGAGGAGAGGTTTTTAGCTGCAGTGAGAATTTGGAGTTGATTAAGGTTGGATTTGAAGAGATACTGGAGAAAGACATGGATTGTAACAAGGTTCATCAAAATAGTGTTCTTGAGTATCTTGAAAGGGTTGATGAATGTAATAGTCTTAGGTTTAAAAGGGGTAAATACGAAGGGTTTTGGGATAGGGAGTAAGTAATTGGTTGGAAGGTGGGGGAAAGAACCGGCGGATTAATTAAAATCGGGCCAA
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00327 Ps_contig_mira-and-tgicl-ass_franssen_3617
SEQUENCE=ATCAAGATCTTCAATACCGAGTAATCTTTTTTCTCTCAACATCTCGGGGATCCTCAATCATTATGGTTAGTTTGACCTCCCCGAATAGCANGTCCCGGTTTTTTTTTAAGAACAAAGACTAACCAAAAACCCTTTGATTTGGGCAATATATTCAAGGTCTTGAGGGGTTGGTTCTTCTTCTTCATCATCATCATCATCCTCCTCAACATCATCATCATCATCATTCAGTTGCTGTGTAGAGGAAACACGGTAGGTACAAGAATCTTCATTCCCAACAGAGGCAACGACGGAAGAAAGTATGGTTGATGTTCGGCGATGAGTGATGCTAAGCTTCGTGAACTGTGCTCCGTTAAGGAAGGNGTGAGAG
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6612
PRIMER_LEFT_PENALTY=0.502921
PRIMER_RIGHT_PENALTY=0.158317
PRIMER_LEFT_SEQUENCE=TTAGTTTGACCTCCCCGAATAG
PRIMER_RIGHT_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT=66,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=59.497
PRIMER_RIGHT_TM=59.842
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.6847
PRIMER_LEFT_1_PENALTY=0.344527
PRIMER_RIGHT_1_PENALTY=0.340219
PRIMER_LEFT_1_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_1_SEQUENCE=TTCACGAAGCTTAGCATCACTC
PRIMER_LEFT_1=148,22
PRIMER_RIGHT_1=338,22
PRIMER_LEFT_1_TM=60.345
PRIMER_RIGHT_1_TM=59.660
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.7491
PRIMER_LEFT_2_PENALTY=0.344527
PRIMER_RIGHT_2_PENALTY=0.404566
PRIMER_LEFT_2_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_2_SEQUENCE=ACCATACTTTCTTCCGTCGTTG
PRIMER_LEFT_2=148,22
PRIMER_RIGHT_2=302,22
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=60.405
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.7491
PRIMER_LEFT_3_PENALTY=0.344527
PRIMER_RIGHT_3_PENALTY=0.404566
PRIMER_LEFT_3_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_3_SEQUENCE=CAACCATACTTTCTTCCGTCGT
PRIMER_LEFT_3=148,22
PRIMER_RIGHT_3=304,22
PRIMER_LEFT_3_TM=60.345
PRIMER_RIGHT_3_TM=60.405
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.7810
PRIMER_LEFT_4_PENALTY=0.502921
PRIMER_RIGHT_4_PENALTY=0.278124
PRIMER_LEFT_4_SEQUENCE=TTAGTTTGACCTCCCCGAATAG
PRIMER_RIGHT_4_SEQUENCE=CACAGCAACTGAATGATGATGA
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.497
PRIMER_RIGHT_4_TM=59.722
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_00328 Ps_contig_mira-and-tgicl-ass_franssen_3617
SEQUENCE=ATCAAGATCTTCAATACCGAGTAATCTTTTTTCTCTCAACATCTCGGGGATCCTCAATCATTATGGTTAGTTTGACCTCCCCGAATAGCANGTCCCGGTTTTTTTTTAAGAACAAAGACTAACCAAAAACCCTTTGATTTGGGCAATATATTCAAGGTCTTGAGGGGTTGGTTCTTCTTCTTCATCATCATCATCATCCTCCTCAACATCATCATCATCATCATTCAGTTGCTGTGTAGAGGAAACACGGTAGGTACAAGAATCTTCATTCCCAACAGAGGCAACGACGGAAGAAAGTATGGTTGATGTTCGGCGATGAGTGATGCTAAGCTTCGTGAACTGTGCTCCGTTAAGGAAGGNGTGAGAG
TARGET=184,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6612
PRIMER_LEFT_PENALTY=0.502921
PRIMER_RIGHT_PENALTY=0.158317
PRIMER_LEFT_SEQUENCE=TTAGTTTGACCTCCCCGAATAG
PRIMER_RIGHT_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT=66,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=59.497
PRIMER_RIGHT_TM=59.842
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.6847
PRIMER_LEFT_1_PENALTY=0.344527
PRIMER_RIGHT_1_PENALTY=0.340219
PRIMER_LEFT_1_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_1_SEQUENCE=TTCACGAAGCTTAGCATCACTC
PRIMER_LEFT_1=148,22
PRIMER_RIGHT_1=338,22
PRIMER_LEFT_1_TM=60.345
PRIMER_RIGHT_1_TM=59.660
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.7491
PRIMER_LEFT_2_PENALTY=0.344527
PRIMER_RIGHT_2_PENALTY=0.404566
PRIMER_LEFT_2_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_2_SEQUENCE=ACCATACTTTCTTCCGTCGTTG
PRIMER_LEFT_2=148,22
PRIMER_RIGHT_2=302,22
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=60.405
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.7491
PRIMER_LEFT_3_PENALTY=0.344527
PRIMER_RIGHT_3_PENALTY=0.404566
PRIMER_LEFT_3_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_3_SEQUENCE=CAACCATACTTTCTTCCGTCGT
PRIMER_LEFT_3=148,22
PRIMER_RIGHT_3=304,22
PRIMER_LEFT_3_TM=60.345
PRIMER_RIGHT_3_TM=60.405
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.7810
PRIMER_LEFT_4_PENALTY=0.502921
PRIMER_RIGHT_4_PENALTY=0.278124
PRIMER_LEFT_4_SEQUENCE=TTAGTTTGACCTCCCCGAATAG
PRIMER_RIGHT_4_SEQUENCE=CACAGCAACTGAATGATGATGA
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.497
PRIMER_RIGHT_4_TM=59.722
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_00329 Ps_contig_mira-and-tgicl-ass_franssen_3617
SEQUENCE=ATCAAGATCTTCAATACCGAGTAATCTTTTTTCTCTCAACATCTCGGGGATCCTCAATCATTATGGTTAGTTTGACCTCCCCGAATAGCANGTCCCGGTTTTTTTTTAAGAACAAAGACTAACCAAAAACCCTTTGATTTGGGCAATATATTCAAGGTCTTGAGGGGTTGGTTCTTCTTCTTCATCATCATCATCATCCTCCTCAACATCATCATCATCATCATTCAGTTGCTGTGTAGAGGAAACACGGTAGGTACAAGAATCTTCATTCCCAACAGAGGCAACGACGGAAGAAAGTATGGTTGATGTTCGGCGATGAGTGATGCTAAGCTTCGTGAACTGTGCTCCGTTAAGGAAGGNGTGAGAG
TARGET=207,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6252
PRIMER_LEFT_PENALTY=0.284978
PRIMER_RIGHT_PENALTY=0.340219
PRIMER_LEFT_SEQUENCE=TTCATCATCATCATCATCCTCC
PRIMER_RIGHT_SEQUENCE=TTCACGAAGCTTAGCATCACTC
PRIMER_LEFT=180,22
PRIMER_RIGHT=338,22
PRIMER_LEFT_TM=59.715
PRIMER_RIGHT_TM=59.660
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=159
PRIMER_PAIR_PENALTY_1=0.6612
PRIMER_LEFT_1_PENALTY=0.502921
PRIMER_RIGHT_1_PENALTY=0.158317
PRIMER_LEFT_1_SEQUENCE=TTAGTTTGACCTCCCCGAATAG
PRIMER_RIGHT_1_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=59.497
PRIMER_RIGHT_1_TM=59.842
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.6847
PRIMER_LEFT_2_PENALTY=0.344527
PRIMER_RIGHT_2_PENALTY=0.340219
PRIMER_LEFT_2_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_2_SEQUENCE=TTCACGAAGCTTAGCATCACTC
PRIMER_LEFT_2=148,22
PRIMER_RIGHT_2=338,22
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=59.660
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.6895
PRIMER_LEFT_3_PENALTY=0.284978
PRIMER_RIGHT_3_PENALTY=0.404566
PRIMER_LEFT_3_SEQUENCE=TTCATCATCATCATCATCCTCC
PRIMER_RIGHT_3_SEQUENCE=ACCATACTTTCTTCCGTCGTTG
PRIMER_LEFT_3=180,22
PRIMER_RIGHT_3=302,22
PRIMER_LEFT_3_TM=59.715
PRIMER_RIGHT_3_TM=60.405
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=123
PRIMER_PAIR_PENALTY_4=0.6895
PRIMER_LEFT_4_PENALTY=0.284978
PRIMER_RIGHT_4_PENALTY=0.404566
PRIMER_LEFT_4_SEQUENCE=TTCATCATCATCATCATCCTCC
PRIMER_RIGHT_4_SEQUENCE=CAACCATACTTTCTTCCGTCGT
PRIMER_LEFT_4=180,22
PRIMER_RIGHT_4=304,22
PRIMER_LEFT_4_TM=59.715
PRIMER_RIGHT_4_TM=60.405
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_00330 Ps_contig_mira-and-tgicl-ass_franssen_3619
SEQUENCE=GTGCGGGGACTTCTTCTCAAACTCTCTCCTTCAAATCTCTCCCCCCCTCTCTCTCTTCAACTAAAACAAACACACCATTCACCTTCCCACATTCAAACCAAACATAACACACGAATGGCAATTGAAAACCAAGACAACACCTCTGTCAGAGAAATCAAACCAAAAAACAGAAGAATCATGGGTGCTGGAGGTCCTGATGAAGAGGATGACAGTAGATGGCCACCATGGTTGAAACCTTTGTTGAAAGAAAATTTCTTTGTTCAATGCAAGTTACATGCTGATTCCCACAAAAGTGAATGTAACATGTATTGTTTGGATTGTATGAATGGTCCTATATGTTCTCTCTGTCTTTCTTACCATAAAGAACATCGTTTTATTCAGATCCGAAGATCTTCATACCATGATGTGATTAGGGTGAATGAGATTCAGAAGGTATTGGACATCACTGGTGTTCAAACGTATATTATCAACAGTGCAAGAGTTGTGTTTTTGAATGAAAGGCCTCAGCCAAGGCCGGGGAAAGGTGTCACAAACACTTGTGAAGTTTGCGAGCGTAGTCTTCTCGATTCCTTCCGGTTCTGCTCTCTGGGTTGCAAGATTGTAGGAACATCTAAGAATTTCCAGAAGAAAAAGAAACAAATAACAGCAATGGCATCAGATTCAGAAGATTCATACAGTAGCAACAACAACATTCACAGCCGTCACAAGAAAATCAAAGTTCAAAGCTTCACACCATCAACACCTCCTCCAACTTCTGTTAATTACAGAACAGCCAAGAGAAGAAAGGGAATTCCACATAGAGCCCCATTAGGGGATAGATAGTTGGTACTATTAATAGTTGTTATTTTATTTATTGCTATAATCGACCCTAGTGTACAAGTTAACTTTTAATTTTTTTTAAAAAGTTGTTTTAGGTGGTGTATATAGAC
TARGET=47,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0515
PRIMER_LEFT_PENALTY=1.047917
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=TCTCTCCTTCAAATCTCTCCCC
PRIMER_RIGHT_SEQUENCE=CTTTTGTGGGAATCAGCATGTA
PRIMER_LEFT=22,22
PRIMER_RIGHT=292,22
PRIMER_LEFT_TM=61.048
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=1.0523
PRIMER_LEFT_1_PENALTY=1.047917
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=TCTCTCCTTCAAATCTCTCCCC
PRIMER_RIGHT_1_SEQUENCE=AGCACCCATGATTCTTCTGTTT
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=61.048
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=1.1227
PRIMER_LEFT_2_PENALTY=1.119099
PRIMER_RIGHT_2_PENALTY=0.003589
PRIMER_LEFT_2_SEQUENCE=CGGGGACTTCTTCTCAAACTCT
PRIMER_RIGHT_2_SEQUENCE=CTTTTGTGGGAATCAGCATGTA
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=292,22
PRIMER_LEFT_2_TM=61.119
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=1.1235
PRIMER_LEFT_3_PENALTY=1.119099
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=CGGGGACTTCTTCTCAAACTCT
PRIMER_RIGHT_3_SEQUENCE=AGCACCCATGATTCTTCTGTTT
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=61.119
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=1.1808
PRIMER_LEFT_4_PENALTY=1.047917
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_LEFT_4_SEQUENCE=TCTCTCCTTCAAATCTCTCCCC
PRIMER_RIGHT_4_SEQUENCE=TTTGTGGGAATCAGCATGTAAC
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=290,22
PRIMER_LEFT_4_TM=61.048
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_00331 Ps_contig_mira-and-tgicl-ass_franssen_3627
SEQUENCE=CTTGTGATTTACTCTTACTTCACTTAATGTACCTTTCTATTAGATCTTATTTTGTGATTTTATTTAATTAATTAAGATGTTGATTTTCGGTTACTGTTGGTTTAGGTGCTCTGATTGCTGTTCATTCTATTCTGTTTCATTTTTGGGATAAGTTGGGAGCTGAAGTGAAAAAAGAGGAGAAAGAAGAAAAGCTTGATGGTGATGCTGCTTTGAATAAATTGTTCCGTGATATTTATCAAAATGCAGATGAGGACATGAGGAGAGCCATGAGCTAAGTTCTTTTCNGGAATCAAATGGAACAGTGCTATCAACTGATTAGGAAAGAGGTGGATACGAAAGGAAGGTGGAAGGAACGTCCCTACCGAAGGAAGGAAGTGGGAGGTAGAA
TARGET=64,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00332 Ps_contig_mira-and-tgicl-ass_franssen_3627
SEQUENCE=CTTGTGATTTACTCTTACTTCACTTAATGTACCTTTCTATTAGATCTTATTTTGTGATTTTATTTAATTAATTAAGATGTTGATTTTCGGTTACTGTTGGTTTAGGTGCTCTGATTGCTGTTCATTCTATTCTGTTTCATTTTTGGGATAAGTTGGGAGCTGAAGTGAAAAAAGAGGAGAAAGAAGAAAAGCTTGATGGTGATGCTGCTTTGAATAAATTGTTCCGTGATATTTATCAAAATGCAGATGAGGACATGAGGAGAGCCATGAGCTAAGTTCTTTTCNGGAATCAAATGGAACAGTGCTATCAACTGATTAGGAAAGAGGTGGATACGAAAGGAAGGTGGAAGGAACGTCCCTACCGAAGGAAGGAAGTGGGAGGTAGAA
TARGET=364,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00333 Ps_contig_mira-and-tgicl-ass_franssen_3628
SEQUENCE=TGGTATCAACGCAGAGTCGGGGGGAAGTTTCAACGAACAAGTGAAGAAAGGGATTTGAAGAAGCAGAAGAAGAAAATCAGAGATGGCGAAAGAGCTTGAGAACAAAGCCAAAGAAGCGTTCTTCGATGACGATTTTTCTCTAGCCGTTGATCTCTATTCTCAGGCTATAGAACTCGATCCTGCCAACGCAAATCTCTTCGCCGACAGAGCTCAAGCACATATCAAACTCAACGCTTTCACTGAAGCTGTTTCTGATGCTAATAAGGCTATTCAATTAAACCCTACTTTATCAAAGGCTTATATCCGCAAGGGGACTGCTTGTATTAATCTGGAAGAATATCATACTGCTAAGGTAGCACTTGAAAAGGGTGCTTCGATTGCACCTGCAGATTCCAGGTTTACCAATTTGATTCAACAATGTGAACGCTACATTGCTGAGGAATCAGATAGTCTTACTGGTATCCTACCAACAAATGGGCCCAAAACTTCTGTACCATCTGTTGATGATACCCATATATGTGATAAAGGTGATGGAACAGGAGCATCCAAAGAACCTCAAAGAGACAGTACCACATCTGAAACAAGCGAAGCTGCACAAGTCAGACCAAAATACAGACATGAATATTACCAAAAGCCTGAAGAAGTGGTTGTCACAATATTTGCAAAAGGAATACCAGCAGACAATGTTGTTGTGGACTTCGGTGAACAAATTCTGAGTGTTACCATTAATGTTCCTGGCCAACATGCATATCTTTATCAACCTCGATTGTTTGGAAAGATTATCCCAGCTAAGTCCAAAGTTTTGGTGTTGTCAACCAAAATAGAAGTTCGTCTGGCAAAAGCTGAAGCTATTAATTGGACATCTCTGGAATATTGCAAGGATGTGGTTCCCCAGAAGATAAGCGTGCCTACAATTCAATCTGAAAGGCCTACATATCCATCATCAAAGTCAAGGACTAAGGATTGGGATAAGTTGGAAGCTGAAGTGAAAAAAGAGGAGAAAGAAGAAAAGCTTGATGGTGATGCTGCTTTGAATAAATTGTTCCGTGATATTTATCAAAATGCAGATGAGGACATGAGGAGAGCCATGAGCAAGTCTTTTCTGGAATCAAATGGAACAGTGCTATCAACTGATTGGAAAGAAGTGGGATCAAAGAAGGTGGAAGGAAGTCCTCCAGAAGGAATGGAGGTGAAGAAATGGGAGTATTAGTTCAGTTTCAATTACTGGGTCATATAAGCTTATGCTTTTGAGTTAAAATTCATTTAGCTTTTGTTTTTTGCATAGCCAATTTGTCTGCAATCTCTTGTCATTAGTTTCTATTTATTTATTTTTGTATGATTAAGTTTTATTTTTCCGTTGGGGTAAGTAATTKKAACTTAATTTGCTAGTTTCGGGACCGGGGTAAGTTTAGTTAAATTTTTTATTTATCTTAGGAACGACC
TARGET=1319,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00334 Ps_contig_mira-and-tgicl-ass_franssen_3629
SEQUENCE=TTGTTCTTTATTTSYTAWCTWWSTKATTGGAAAGAAGTGGGATCAAAGAAGGTGGAAGGAAGTCCTCCAGAAGGAATGGAGGTGAAGAAATGGGAGTATTAGTTCAGTTTCAATTACTGGGTCATATAAGCTTATGCTTTTGAGTTAAAATTCATTTAGCTTTTGTTTTTTGCATAGCCAATTTGTCTGCAATCTCTTGTCATTAGTTTCTATTTATTTATTTTTGTATGATAAGTTATTTTCCTGGGAGAATTGACTAATTTGCATTTGCCGGGGAAGTGAATTTTATTTATTCTCTTGGTCTTTGTTGATTATAGTTATCGGTTAACTTCTGATAATACCCAGACTTGTTTTTATTTTATATTGACATTGGAACTTATATCTTGTAAATTTGACTGMAAAAA
TARGET=211,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00335 Ps_contig_mira-and-tgicl-ass_franssen_3647
SEQUENCE=AGTTGTTCAAGCTTTTGAGCAACTTCAGCCATGTCTGATGATTTCACCTTGTAACCAACCACCGCTAAAAGCTCATCCATACCACCGTCGTCTTCCCAACAAATCTCCGATTTACCAGTCATACTCGCATCTTCGTGTTCTTGGTTATGCTCTCTCTTCATCTTCTTACACTTCTCTCTCTCTCTCACTCACACACAAGTTCTATATTCTGTTTTCTGATTGTAATTTGTGACTTTCTCTTTTCTTTCTCTGTCTTTTTTTTTTTTCTCACTACTGCTTCTAAACTTTCTTCATCAACACAATCATATACTCCGTMAAACTCATATATAT
TARGET=173,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00336 Ps_contig_mira-and-tgicl-ass_franssen_3662
SEQUENCE=CGAAGGTTCTCGAAATCTGGTGGCGGAAACTACGTAAWWCAACCAAAAATCGGGAAAAATAGCAACCTTTGGATCAAGATTGTAATTTAGAAACTCACCTTCGGGCGCCGGCAAATCTCCTCCGACGGCCGCAAGAATCAACATGATCCAACTCCTCCAATCTACGTCGTCATCATTGCTATGTTGGGAAGAACTCGAAGATACTTGTGTTGGCGATGGAGAGAGAATGGTTGTCTGGTTCGTATGCGTCGCATAGAGATGAAGATGAAGGTCGTCTGGACGTRGACGGGTAAGCTCTCTCTCGATTCTCGTTTCCGAWTTTTTGTTTCAGATTCCTTCGCTTTTCCTTTTCGATTCCGTTGCTCTTCTTCTTCTATTTTTTTATTTCTGTTGCAGGGTGGTGGATCATTGTGTTGGAGGTTGTTGTTGGAGATAGAATGGAGTTGTCGTCGATGGTGATGGATGACAGTGGATGAGTCTCGGGTGAATCTGGAGTAACCGAAGGTGAAGAAACCACGCTCAGGAAGTTTGAAGCAGTGGTTATTGAAGAAGGTCGGAGGGTTATGAAAATTTCATATGATGTTGTTGTAGAAAAAAACGGATTGGTTATAAGGAGGTGGAAGTTAGTTCGTGGAATGAATGCTTTGGCAACTGATCTCGTTTAGCTTCTCAATTCTATTCCTGTTCTTTTTTTGCTGTTGTTTCTGTTATGTTTTTTTATTTATTTCTACAGGTTATGAGCTCGAAAGAGTAGTAGTTAGCGAAACTCCGGAAGCTGGTTTTTAGTAGGAATTGA
TARGET=364,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00337 Ps_contig_mira-and-tgicl-ass_franssen_3679
SEQUENCE=CTAAAAACCACCATCTATGCGAAACCACCACCTTGATAGTAACCCCGACAAAACGAAAGAAAATCGCACAAGAAGAAGAAGCAGAGATTAGGACAAGAAGAAAGCAGAGCAAGGGGAAACGATAAGGTATACCTTATACACTGCAGATTCGGTTGGTTTATGCGCTTTGCCTTTGTATAGATCCATGACTCTTAGGGCATTTTGATTTTGTTTTGATGTATGGTGATAACTGTTGATTTATTTTTTACATTTGTTGGATGAATTGTGTTTGTTGCAAACCATGTGAGGTTTGATTCCGACTTTGAAGGTACTGAGAACGAAAATCTGGAGTTCGGTTTTAACATGCGGAATTTGTTTCGCCTTTAAGGACTCTGATCCTTCCAATTGTTGTTCATTTAACGTTAGAATCATGTCTGGTTGGGGATTTGGGTATAGGATCTCTGTTCAGTAGAAGATTTTAGGAAAGTTGGTTCTTAGACTTTGTGTTTGTGGGTTCGGTTGTCCGGAGAAAATGGTGGTAACCACCGCAGACCACCGACTTTTTGTTCTTCTTCCCCGGAGAGGATTCAGAATGAGGGAGAAAGTATCCTAACGATAAGAAGGGAGGCACAATTAGGGAAGAGAAAGAATTGTTTGGTCTTTTAAGTCTGAATTTCATTTATCTTAATTACAADAACS
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00338 Ps_contig_mira-and-tgicl-ass_franssen_3681
SEQUENCE=TTGGTATTCGAATTTACTGTTGCTGTACATCGATCTCCGTTTTCTGTTTGGATTTGCAGTGGTTGATGTGGAGAGGAGGTGGACGATGGTGCGATTGGTAGAGTTGTTGTAGACTGGAAATGGACGTTTGATTGGTAAGGTTTTTGTTTAAAGGTTGAAAGTTTTGTTACGGTGATGACTGGTTGGAAGAAGAAGAAGAATCTGGAATCGTTGCAGAGTATTTGGAGAGTGAAAGAATGAAGAAGAAAAAGG
TARGET=186,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4876
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.483253
PRIMER_LEFT_SEQUENCE=ACATCGATCTCCGTTTTCTGTT
PRIMER_RIGHT_SEQUENCE=CTCTCCAAATACTCTGCAACGA
PRIMER_LEFT=26,22
PRIMER_RIGHT=228,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.517
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=0.5135
PRIMER_LEFT_1_PENALTY=0.030276
PRIMER_RIGHT_1_PENALTY=0.483253
PRIMER_LEFT_1_SEQUENCE=TGTTGTAGACTGGAAATGGACG
PRIMER_RIGHT_1_SEQUENCE=CTCTCCAAATACTCTGCAACGA
PRIMER_LEFT_1=104,22
PRIMER_RIGHT_1=228,22
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=59.517
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=0.5922
PRIMER_LEFT_2_PENALTY=0.108929
PRIMER_RIGHT_2_PENALTY=0.483253
PRIMER_LEFT_2_SEQUENCE=AATGGACGTTTGATTGGTAAGG
PRIMER_RIGHT_2_SEQUENCE=CTCTCCAAATACTCTGCAACGA
PRIMER_LEFT_2=118,22
PRIMER_RIGHT_2=228,22
PRIMER_LEFT_2_TM=60.109
PRIMER_RIGHT_2_TM=59.517
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=0.6131
PRIMER_LEFT_3_PENALTY=0.129818
PRIMER_RIGHT_3_PENALTY=0.483253
PRIMER_LEFT_3_SEQUENCE=AGACTGGAAATGGACGTTTGAT
PRIMER_RIGHT_3_SEQUENCE=CTCTCCAAATACTCTGCAACGA
PRIMER_LEFT_3=110,22
PRIMER_RIGHT_3=228,22
PRIMER_LEFT_3_TM=59.870
PRIMER_RIGHT_3_TM=59.517
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=0.7117
PRIMER_LEFT_4_PENALTY=0.228464
PRIMER_RIGHT_4_PENALTY=0.483253
PRIMER_LEFT_4_SEQUENCE=GACTGGAAATGGACGTTTGATT
PRIMER_RIGHT_4_SEQUENCE=CTCTCCAAATACTCTGCAACGA
PRIMER_LEFT_4=111,22
PRIMER_RIGHT_4=228,22
PRIMER_LEFT_4_TM=60.228
PRIMER_RIGHT_4_TM=59.517
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_00339 Ps_contig_mira-and-tgicl-ass_franssen_3688
SEQUENCE=ACTTTTTAAAACTTTTACTATATCTTCWWTTTCCGCACTTGGTTTTTCTCACATATATACAATCTTGCAGAAAATAATTTAATGCTAACCAAATAAAAAAATAATTACTAGCTAATTAGCCTATGGAGACACTCAAAATCAGTGTTTTCACAGCACATGTAAGGACAACAAAATAAGGAGAATAAATTTCAGGTCCTTCCAGGATAAAATCTTACTCTTTGCTTCCAACTTTCCGCTTCTTTCGTTTGGAAGTTGGCGTCGAACTGTAAACATAAACAAAAATATTAAAAACAGCTCAATTGCAGATGATAGCATCATTAAATTTCATGAATGAATGAATTTATAAGGTGATGGATACCTCCCATTTATAAACACATTTTCAGGTCATAATGTAGAATAGTTAACACACCCCTCAAATTCAGGACTGAACCTTTGAAGTGTGATTCAACCGATCAAGGATAGACCTTCGTAGCTACTTAGAATTTAACTTGGGCCTAACTTAACTTTACACAATTGACTTGTAAGATGAGGGAAATAACTCAAAAACTTTCAGGTCATATATCACCCAACGTAATACTGCCAGCACACACCCATCTGACACTGAATACTAGACTTTTGGTGTCTCATCCGATTGCAAGAGGCGCAACGCATCTTGGAAACTCTCATCCGATCACTCAAGTCACACTTCAGATATTCAATCTTTGGACTCGAGAGAGTTGTATTAATAGTATCATATTTGACGATAAGACCTGAAAATGTGTTTACATAAGTTGACTCCCCCGCCTTACAAGCTAATTTTGTAGGGTATGTAACCTAAATTTAAGACAAATAAATCAAAATCTCATTAGGTTTGTTCTCTTTTGTTTATATGGTTCTAGTGGAAATTTGCTGTATTCATTGATTATTGAATTCAATCAGTTGGTTAACTGCTACATATTTTACAGCTGCTCCAGAGGAAAATGGATCATATGCCATGTATCTATTTATAATAAAGCTCAAGTTCTAACCATGGAAAATAATTTGCACACTCAATCAGACAGTAATCATATATTTAGTCCATTCCGAAAATATTAGAGTTTGGCATAGGTACCTTGTTAGAATAGGTTGTATATCAGCACCAACAGTTTTACTAAGCTCTTCAATAGTTGCAGAAACCAACATTACTACATTTAAGTATTAGTTGCAAG
TARGET=327,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00340 Ps_contig_mira-and-tgicl-ass_franssen_3692
SEQUENCE=TACACTCACTCACTCACCACTAGCTTTCTACCATCCCCTTTTCCCTTTACTTAACTAGAAAAACCAAAAGTAACTATCAATTTGTAAGAACTCTTCTTCTTTCACTATACATGATGATCAATCAGTCTCTTTCACAGTCCTGCATCAAACGGCACACCACTACCAGAAATCCATGACTCACCAGCCAAGAAATTACCAACAGAAAATTTAACTGCTTCCGAAGGATTTGTAATTACATGAAAACCAGACCAATTCACTCTCCCATCTGTATTAGCACCATCACCAACATTCATATATTCTGCATAATAAAGTGTGCTCAAGGCAAAACCACCACTCCATGGAGCCCAACCTTGTGAATTTATAACACCGTCAATATTACTTTTCATTACAACCGTTCTTGAATACTTCTGCCACGGCCTTCCTAGATAACTTTTCACTGAATTTTGAGATGCTTTCAAATCACCTGCTGCGGTGATACGGCAGTTATGGATCACTATTCCGGTATTCTCGTTTGGATCTGTTCTTCCCTGTGCCGTTACGGTGTTCTGTTGGTTGCCCATAGGTTTTCTAACTAAGATGTTGCAGTTTTGAAGAACTGTAACGGCGTTRCCAAAGATGAAATCGACTGTTCCGTAGATGTTACAGTCACGGTAGAATTGACGGTTGGCATAGACGTATAGAGTGTCCTGATAGCCTTTGAAGGAACATCGGTAAGAAAACGGAGTGATCGGCG
TARGET=3,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00341 Ps_contig_mira-and-tgicl-ass_franssen_3699
SEQUENCE=GAATCTAAATGAAAGTGAGTTATGAGAATGAAAGGAAGGAACCRATGARAGGAGGAACATCATGTTTGCTGCTGCTGCAGCAGCTGTTTGTTCTTGTTGCTGGGATGGTCTTTGGCTGATGAACCTAACGTGGTTCTCCAGAAGACTAAAGAAAGCATATGCCACCGTTGTTTGGTACGTTACCA
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00342 Ps_contig_mira-and-tgicl-ass_franssen_3704
SEQUENCE=TAAATAAGTACTAAACAAACAAACTAACCACTAAACCTTACCATCCAAATTAACAAAGTAAAGGTAGCTCAAACAATAGCTATGTTCATGGAATCATGTAAAAAAACTTTTAATAGTCAATTTATTGAATGAAACAAAGTAACCATTTGATTAACGAGCATTCAGGAACACAGATTCTTCAAGTTCTGATGTTCCTGTTTTTGTAGCTGGAGCCTAAGAAAATCAATGGCCGCTTCCGTGATCATCATGACCCCTCCCAGGGATGCCCTCCAGCCCAGTACGTACGGGAGCATTCTTGTCTTTCGCCCTGTACATACCAACCCCAAAACCACATGACAGCGCACAACGTCCGTTTGGCCGGTAGGTACGGTGTCGCCTAGTCGTCGTTTAGGTTG
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00343 Ps_contig_mira-and-tgicl-ass_franssen_3800
SEQUENCE=GAGTTGTGAGGAGAAAAAAGAAGAGAGGKTTAATTATTTATGAAGAAAATAAAGAGAGAGTAAGATTGGATTTATGAAGAGAGAGAGGAGTGTGGGTATAGTATRKYMMSSTAGA
TARGET=78,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00344 Ps_contig_mira-and-tgicl-ass_franssen_3802
SEQUENCE=CCTCTCTCKMWYCATAAATCCAATCTTACTCTCTCTTTATTTTCTTCATAAATAATTAAACCCCTCTCTCTTTTTCTCCTCACAACTCAACTGTTACCATTTTCTTCTTCTTCTTCTTCTTCCTTCCACCCTCAATATGGATTGCTGCATAATCAGTAAACTCAAATCCGAACCTCTCTGGTTCCTCCTTCTCTTCTCACTCGGTCTCACCACCATTCTCAGATTCACTCTAACATTCCTCAACTGGGTCTATGTTAATTTTCTCAGACAACCCAAAAACCTACAAAAAGTACGGATCTTGGGCACTCGTTACCGGATTCTTCTGGTTTAGG
TARGET=102,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00345 Ps_contig_mira-and-tgicl-ass_franssen_3805
SEQUENCE=GGTAGTCAACGACAGAGTAAACAACGGGGGAAGGTTAATTATTTAAAAACCCTAAAACGAATAAATAGCTCACCACCCCTCSGGTGTCATGTTCTTTTTCTAACTCTCTCTCTCTTTCTTCTCACCTTCCTTCTTTCTTCTTCTCCTCCTTTTCCATCTCTATATCTTCCATTTCTCCAATTTTCTCCTCCCTTATTGACCTTTGGTCACCACTCTCAAATACCCATTTCTCTATTTTCTGGTTTAAAGGTCTGAAAAAAGGTTGATAACTCTTTGAGTTGAGTTGGTGTTTGAAGCTAAACAAAGGTTTTTGAAACCCAATTTACAGGTTGAAGCTGATTTTTCTGGAAATTTGGGCCATCTTTAGCGGTGTTTTCCTGAGAAAAGATGATSATGTYWGTCTACGACACAAATATCACAGG
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00346 Ps_contig_mira-and-tgicl-ass_franssen_3811
SEQUENCE=ATTGAGGTAGTTAGATTTTTGCTTAGAGAAGTGTAGAGTAGGAACTAGTCTTGTTGTATTCCACAGTTTTCTCTTACTTTTCCAAGCTTTCGGACCATCTTGTCTTCACTACTATTTAGACCATCTCTAATAGAGGAGTTGTTGTTGTTTTATGTGTGTTTTTAAGCATAAGAATCTGTTGAGATGACATGTTGTGTTCTTCATATGTAGTAATAACATTCTTCAATAAAATAGTTGGTTCAAATTGWC
TARGET=138,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00347 Ps_contig_mira-and-tgicl-ass_franssen_3812
SEQUENCE=CYAATTGAACCAACAATTTTATTGAAGAATGTTATTACTACATATGAAGAACACAACATGTCATCTCAACAGATTCTTATGCTTAAAAACACACGTAAAACAACAACAACTCCTCTATTAGAGATGGTCTAAATAGTAGTAAAGACCAGATGGTCCGAAAGCTTGGAAAAATAAAAGAAACCTGAAAACTGTGGAATACAACAAGACTAGTTCCTACTCTACACTTCTCTAAGCAAAAATCTAACTACCACAATGTCAAAGCTAATTTATTACAAATGATTGACCAAAATTCATACATCCCATGTTCACCTTCCTCTTTCTTTTTTTCTTTCTTTCTTTTAGTAAATCATGTCAACCAATCACGAACTCCTCGATATTTCTTTCAACAAGAAACTCTCACTGGAGTGATTGGTCGACGCTCGTCTGCTCATTTTATGTCTCTTCAAGTGCCGACAACACTGGCAGCAACTCAAGCATCATCACTGTTTCAACTTAGGTTTTGAAATGTTTTCAACTTACTTAAAGCTCCGGCATGCCATTTGATCGCGATGAATATTCTGTCACCAACTTCAAAAACATGGATGACTTGTCTTCTAATAGCCGCAATGGAGTGTCAAACTCTGCGTTGATACCACTYGTCTT
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00348 Ps_contig_mira-and-tgicl-ass_franssen_3812
SEQUENCE=CYAATTGAACCAACAATTTTATTGAAGAATGTTATTACTACATATGAAGAACACAACATGTCATCTCAACAGATTCTTATGCTTAAAAACACACGTAAAACAACAACAACTCCTCTATTAGAGATGGTCTAAATAGTAGTAAAGACCAGATGGTCCGAAAGCTTGGAAAAATAAAAGAAACCTGAAAACTGTGGAATACAACAAGACTAGTTCCTACTCTACACTTCTCTAAGCAAAAATCTAACTACCACAATGTCAAAGCTAATTTATTACAAATGATTGACCAAAATTCATACATCCCATGTTCACCTTCCTCTTTCTTTTTTTCTTTCTTTCTTTTAGTAAATCATGTCAACCAATCACGAACTCCTCGATATTTCTTTCAACAAGAAACTCTCACTGGAGTGATTGGTCGACGCTCGTCTGCTCATTTTATGTCTCTTCAAGTGCCGACAACACTGGCAGCAACTCAAGCATCATCACTGTTTCAACTTAGGTTTTGAAATGTTTTCAACTTACTTAAAGCTCCGGCATGCCATTTGATCGCGATGAATATTCTGTCACCAACTTCAAAAACATGGATGACTTGTCTTCTAATAGCCGCAATGGAGTGTCAAACTCTGCGTTGATACCACTYGTCTT
TARGET=325,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00349 Ps_contig_mira-and-tgicl-ass_franssen_3823
SEQUENCE=GGAGTAGAATAGTGTGACCACTTATTTGATACTTCCTTACGTTCCTGCTTCTTTTTTCTTAACTGCTCTAGGTGCTAGACCAATCGCTTCGGAGCTTTTCTATGATCCTTAGCCTCCTGCATGTATCGATAAACATCTCCCAAGGAACATCACCGACAAGCATCCAGTCACCGTCTTTATCCTCGTAAGTAATAACATATTCTGACCCATGAAGCAGATCTCTCAGCTTTGTTTCATTCAGCATCTGATTTCCATGAGATTCACATTGTCCTGAATATAACATCGACATAAGCAGTAACTTGATCCAGACACAAAATTTGCCGAAAATCATTAGAAAGACTATTTATTTTTCTTACGAAAAAATGAACCTATTCCTACCTACCTATGGTGAAACAGCTGAACATCTTCTCAAGAGATGAAGAGAGTTCTGAATATGCAGTGTAGTTCTTCAGATCTACTTTTCTCAAATAAGGAGCACCATCCATGCTAACCTTCACAAACACGGCGCCTCCTAATCCCAGTTTTCCATCAGCCTCTTCGGTGTTCTTTGAAGCAGTTGTCAAGGAATTTTTGCGAAATGATCTTATTGGAGGCCAACCAACAACCT
TARGET=374,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2375
PRIMER_LEFT_PENALTY=0.203367
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=TTCCATGAGATTCACATTGTCC
PRIMER_RIGHT_SEQUENCE=GCTGATGGAAAACTGGGATTAG
PRIMER_LEFT=249,22
PRIMER_RIGHT=532,22
PRIMER_LEFT_TM=59.797
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.3739
PRIMER_LEFT_1_PENALTY=0.203367
PRIMER_RIGHT_1_PENALTY=0.170538
PRIMER_LEFT_1_SEQUENCE=TTCCATGAGATTCACATTGTCC
PRIMER_RIGHT_1_SEQUENCE=CTGATGGAAAACTGGGATTAGG
PRIMER_LEFT_1=249,22
PRIMER_RIGHT_1=531,22
PRIMER_LEFT_1_TM=59.797
PRIMER_RIGHT_1_TM=59.829
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.4092
PRIMER_LEFT_2_PENALTY=0.375090
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=CCATGAGATTCACATTGTCCTG
PRIMER_RIGHT_2_SEQUENCE=GCTGATGGAAAACTGGGATTAG
PRIMER_LEFT_2=251,22
PRIMER_RIGHT_2=532,22
PRIMER_LEFT_2_TM=60.375
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.5456
PRIMER_LEFT_3_PENALTY=0.375090
PRIMER_RIGHT_3_PENALTY=0.170538
PRIMER_LEFT_3_SEQUENCE=CCATGAGATTCACATTGTCCTG
PRIMER_RIGHT_3_SEQUENCE=CTGATGGAAAACTGGGATTAGG
PRIMER_LEFT_3=251,22
PRIMER_RIGHT_3=531,22
PRIMER_LEFT_3_TM=60.375
PRIMER_RIGHT_3_TM=59.829
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.5533
PRIMER_LEFT_4_PENALTY=0.102129
PRIMER_RIGHT_4_PENALTY=0.451213
PRIMER_LEFT_4_SEQUENCE=TGAAGCAGATCTCTCAGCTTTG
PRIMER_RIGHT_4_SEQUENCE=GGATGGTGCTCCTTATTTGAGA
PRIMER_LEFT_4=209,22
PRIMER_RIGHT_4=482,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=60.451
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_00350 Ps_contig_mira-and-tgicl-ass_franssen_3837
SEQUENCE=TGCTAGATTGTGTTTTGTTGAAATAAAAATGTCATTGCCTTGAGGTTATATATATATATATATATTGTATGAGACAATTAAGGTCATTCTTATGTTTCATTGAGAGTGAAAATTCATAATAAAAAAGGAATAACTACTTTACTTTTACTGATG
TARGET=47,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00351 Ps_contig_mira-and-tgicl-ass_franssen_3840
SEQUENCE=GTAACTTAAGAGCAAGTGTGACATTAATACTAAAACCAAGGTTAGGGTCATCATGATCCTTAATAAGAATACACAAACATCTTTTACTCTTATTAACAACATCCTTAACACCAGTGCAACAATCTATGGTTGGTGTTTTGGACTGACCACTTACAAAAGGAAGACAATTGGCCAAACCTATGAGTTTGTCTGTGCATTCTGCTTTGTCTTGGTTCACATCTGAGGTTACAAGACCAAACATAAGGACTAGGAAAATCAAGGAAGAGAAGAGTGATGATAGATTTTTTGCACTTTTGTAAACCATTTTGATGATGAAAGAGTAGCTCAGAAAGTGAGAGAGAGAGAGGGAGAGACAAAACAAACAAGAAATGTGTGATATAGAATGGAAACACCTCCCCGACCACTGTCTTCG
TARGET=334,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8981
PRIMER_LEFT_PENALTY=0.059316
PRIMER_RIGHT_PENALTY=1.838812
PRIMER_LEFT_SEQUENCE=TCTGTGCATTCTGCTTTGTCTT
PRIMER_RIGHT_SEQUENCE=GGGGAGGTGTTTCCATTCTAT
PRIMER_LEFT=188,22
PRIMER_RIGHT=397,21
PRIMER_LEFT_TM=60.059
PRIMER_RIGHT_TM=59.161
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=1.9185
PRIMER_LEFT_1_PENALTY=0.079714
PRIMER_RIGHT_1_PENALTY=1.838812
PRIMER_LEFT_1_SEQUENCE=ACACCAGTGCAACAATCTATGG
PRIMER_RIGHT_1_SEQUENCE=GGGGAGGTGTTTCCATTCTAT
PRIMER_LEFT_1=107,22
PRIMER_RIGHT_1=397,21
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.161
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=291
PRIMER_PAIR_PENALTY_2=1.9873
PRIMER_LEFT_2_PENALTY=0.148509
PRIMER_RIGHT_2_PENALTY=1.838812
PRIMER_LEFT_2_SEQUENCE=TTGTCTTGGTTCACATCTGAGG
PRIMER_RIGHT_2_SEQUENCE=GGGGAGGTGTTTCCATTCTAT
PRIMER_LEFT_2=203,22
PRIMER_RIGHT_2=397,21
PRIMER_LEFT_2_TM=60.149
PRIMER_RIGHT_2_TM=59.161
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=2.0587
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=1.838812
PRIMER_LEFT_3_SEQUENCE=CTGCTTTGTCTTGGTTCACATC
PRIMER_RIGHT_3_SEQUENCE=GGGGAGGTGTTTCCATTCTAT
PRIMER_LEFT_3=198,22
PRIMER_RIGHT_3=397,21
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=59.161
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=2.0587
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=1.838812
PRIMER_LEFT_4_SEQUENCE=TGCTTTGTCTTGGTTCACATCT
PRIMER_RIGHT_4_SEQUENCE=GGGGAGGTGTTTCCATTCTAT
PRIMER_LEFT_4=199,22
PRIMER_RIGHT_4=397,21
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=59.161
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_00352 Ps_contig_mira-and-tgicl-ass_franssen_3847
SEQUENCE=CCAATCATCCACAAAATTAGCTTGTCCTATATAAAAAATAAAAATCGAAATATATAATAAATTGGCTAAACCATCTTCTATCTAACCCAACTCAAATAAATCCATTTACACTACTCAACTAAAAAAAACTTTAAGTCCTTTTAATTTTTAAATGTAATAATAATCTTGAAAATTGAAATACTAGTATTATTATTATTACTAAATATTTTAAAAACCGAACCAGCAAAACTATGGATCGGGTTCAACAATGTTAAACTGTTCGTTTCAGAATAAAGCCTGAATCAAACGGTTTAAATAATCAAACCCTAGATATCGTCGGTTGATTCGATTTTTAGAATATCGATTAAAAGGTTACTTAATAGTTGAATTGTCTTCCGGTGTAGGTTTGACCAAAAGACCAACTAGCTGGAGCAGCATTGAATGAAACCACACTATGACCATTACTTGTGGTGACAACAAAGGAAAGACTTTGTCCATTAAGGTAAGAGTTACTTTGCCAATTTTCGTCCCCCCAACTTTCTTTGACATGGCTTGCCATCTTGTTCTTGAACCTTTTATGGCCACAGAATGTACATCACCAGCACCTCCAACATTTGTCACAAGTACTAATTGAGAAGAATGT
TARGET=185,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7655
PRIMER_LEFT_PENALTY=1.370891
PRIMER_RIGHT_PENALTY=0.394568
PRIMER_LEFT_SEQUENCE=CATCCACAAAATTAGCTTGTCC
PRIMER_RIGHT_SEQUENCE=CATAGTTTTGCTGGTTCGGTTT
PRIMER_LEFT=5,22
PRIMER_RIGHT=232,22
PRIMER_LEFT_TM=58.629
PRIMER_RIGHT_TM=60.395
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=1.7727
PRIMER_LEFT_1_PENALTY=1.370891
PRIMER_RIGHT_1_PENALTY=0.401769
PRIMER_LEFT_1_SEQUENCE=CATCCACAAAATTAGCTTGTCC
PRIMER_RIGHT_1_SEQUENCE=ATTGTTGAACCCGATCCATAGT
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=58.629
PRIMER_RIGHT_1_TM=59.598
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=1.9636
PRIMER_LEFT_2_PENALTY=1.370891
PRIMER_RIGHT_2_PENALTY=0.592680
PRIMER_LEFT_2_SEQUENCE=CATCCACAAAATTAGCTTGTCC
PRIMER_RIGHT_2_SEQUENCE=TGTTGAACCCGATCCATAGTTT
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=58.629
PRIMER_RIGHT_2_TM=60.593
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=1.9636
PRIMER_LEFT_3_PENALTY=1.370891
PRIMER_RIGHT_3_PENALTY=0.592680
PRIMER_LEFT_3_SEQUENCE=CATCCACAAAATTAGCTTGTCC
PRIMER_RIGHT_3_SEQUENCE=TTGTTGAACCCGATCCATAGTT
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=247,22
PRIMER_LEFT_3_TM=58.629
PRIMER_RIGHT_3_TM=60.593
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=2.1253
PRIMER_LEFT_4_PENALTY=1.370891
PRIMER_RIGHT_4_PENALTY=0.754440
PRIMER_LEFT_4_SEQUENCE=CATCCACAAAATTAGCTTGTCC
PRIMER_RIGHT_4_SEQUENCE=GTTGAACCCGATCCATAGTTTT
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=245,22
PRIMER_LEFT_4_TM=58.629
PRIMER_RIGHT_4_TM=59.246
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_00353 Ps_contig_mira-and-tgicl-ass_franssen_3850
SEQUENCE=GATAAACAGAATTTCGAGCAGGAATTTCCAATATTACCCTTTTCTCTTCCTCAAAATCTCGTTTCGCAATAGCATCTTTTGATTCCCACAGAAACACGCGCTCTCTCTCTCTCTCTTTCTCTCACTTCACGATGGAGAAAGCAGAGAGCTCCACCGCCTCCGCCTCCACCTCCGACCAAGACTCCGACGAAAAGCACCGCACACAACACCACCTAACAGTTCCATCAGGTCTAACTCAACACGAGTTCGACTCACTCATCTCTTTCGTCGCTGAACACCACACCTACCTCATAGGTCCCGGCCAATGCTCCACCTTGCTCGCTCAACGAATCCACGCGCCTCCTGAAACCGTCTGGTCCGTCGTACGCAGCTTCGACAAACCACAAATCTACWATAGACTCAATCCAAACTCACAATCACAATTACAAT
TARGET=101,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00354 Ps_contig_mira-and-tgicl-ass_franssen_3867
SEQUENCE=GTCGGGGAACCCTAAACTAARYCSTSGAAACAATTGTAAATTCCATTCCCAACAAACAGGGGTACACCACACCACACCGCACCGCATGACCAACCTCGCCGTCCCCCTCCTCCTCCTAACGGCGGCGCTAACCCTCTCCGCCGAACCAACCGCCTACGAAGCCCTTCGAAACTTCAATTTTCCAGAGGGAATTCTCCCACAAGGCGTAACACGCTACGAACTCGACGAATCAAGTGGCAATTTCCGCGCCGATCTGAACGGTTCGTGCCGTTTCTCCTTACAAGGTTCGTATC
TARGET=64,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00355 Ps_contig_mira-and-tgicl-ass_franssen_3867
SEQUENCE=GTCGGGGAACCCTAAACTAARYCSTSGAAACAATTGTAAATTCCATTCCCAACAAACAGGGGTACACCACACCACACCGCACCGCATGACCAACCTCGCCGTCCCCCTCCTCCTCCTAACGGCGGCGCTAACCCTCTCCGCCGAACCAACCGCCTACGAAGCCCTTCGAAACTTCAATTTTCCAGAGGGAATTCTCCCACAAGGCGTAACACGCTACGAACTCGACGAATCAAGTGGCAATTTCCGCGCCGATCTGAACGGTTCGTGCCGTTTCTCCTTACAAGGTTCGTATC
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00356 Ps_contig_mira-and-tgicl-ass_franssen_3888
SEQUENCE=GGTAGAACATAATTAAAATTTTGACCAAACAATAGAAACTCAATAAGAATTGTTACATTATTACAATATATATATTGATTGTTTGTCCTACAAAATACAACAGTACTTCTTTTCTTCGTACGGTAATACTCGAAGCTCGCAGTAAGATCTGATAGCCATTGACAGACATTGTTTCTCGAGGTTTTCTGATTTCTGCACAAAGCTCTGCAAGTCATCCCTATGAGAAACTCTTTCAACCTGCAGTTGAAAAGGTTGTAGCACAGTTTA
TARGET=66,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00357 Ps_contig_mira-and-tgicl-ass_franssen_3907
SEQUENCE=AGTTTGGCACCTATCCAATATTTTWCCAAATAACCTTGGCTGAACTAGATAGAGTTGTCTACTCAGAACTTCTGTATGATAGCATCATCATCATCTANTAGTAATTTNCTCTACATTCAATAGCTAGAAACCATC
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00358 Ps_contig_mira-and-tgicl-ass_franssen_3914
SEQUENCE=TAAACAGCTATGTAACCGATCCAGTTAGACTCACGGTCCCAACTCTCACGGGAAAGCGAGTGAAGCAAGAAGGCTTCAGGAACAGAGACACGTGCAGTAGCGTAAAGGAAAGAAACAACAGTGTAGTGTGTTGGGTTTTCAAGCATGACTTTGTTAAAGAACGTGGATTTTCCGTAACGGCTTGAACCGCAGTAGATAGAGTGTTGGTCGTTGTTGAAAGCATCGTAGGTTGTTTGTATGAAGTCACCGCAACGGAGTATGAGGTTGCGGAGGTTGAGGTCTAGTGGGTTTAATAGTGTTTCCCAGTTGTTGCTTCCTAGGAGTTGGTTCCATGTGTCTTGGTTTGTTGTTGTTGTTGATGTTGTTGATAATGTTGGTGGTGGTGGTGTTGTTTCTGTGTCTGTTGCCATGTTTATGGGACTTTTTTTGTGTGATAATCCCATGTTATAGGGAGTGTGTCTGTCTGTTTTTTTGAGGGAGTGGATATATATTGTGAGGAGTTGATGAGGGAAGAGAAGTGTGTGGTGAGA
TARGET=344,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1369
PRIMER_LEFT_PENALTY=0.121635
PRIMER_RIGHT_PENALTY=0.015253
PRIMER_LEFT_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_SEQUENCE=ACCACCACCAACATTATCAACA
PRIMER_LEFT=152,22
PRIMER_RIGHT=383,22
PRIMER_LEFT_TM=59.878
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.1369
PRIMER_LEFT_1_PENALTY=0.121635
PRIMER_RIGHT_1_PENALTY=0.015253
PRIMER_LEFT_1_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_1_SEQUENCE=CACCACCACCAACATTATCAAC
PRIMER_LEFT_1=152,22
PRIMER_RIGHT_1=384,22
PRIMER_LEFT_1_TM=59.878
PRIMER_RIGHT_1_TM=60.015
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.2024
PRIMER_LEFT_2_PENALTY=0.121635
PRIMER_RIGHT_2_PENALTY=0.080808
PRIMER_LEFT_2_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_2_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT_2=152,22
PRIMER_RIGHT_2=411,22
PRIMER_LEFT_2_TM=59.878
PRIMER_RIGHT_2_TM=60.081
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.2374
PRIMER_LEFT_3_PENALTY=0.121635
PRIMER_RIGHT_3_PENALTY=0.115769
PRIMER_LEFT_3_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_3_SEQUENCE=AAAGTCCCATAAACATGGCAAC
PRIMER_LEFT_3=152,22
PRIMER_RIGHT_3=423,22
PRIMER_LEFT_3_TM=59.878
PRIMER_RIGHT_3_TM=60.116
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=272
PRIMER_PAIR_PENALTY_4=0.2454
PRIMER_LEFT_4_PENALTY=0.164560
PRIMER_RIGHT_4_PENALTY=0.080808
PRIMER_LEFT_4_SEQUENCE=GTTGTTGCTTCCTAGGAGTTGG
PRIMER_RIGHT_4_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT_4=305,22
PRIMER_RIGHT_4=411,22
PRIMER_LEFT_4_TM=60.165
PRIMER_RIGHT_4_TM=60.081
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=107
=
PRIMER_SEQUENCE_ID=SSR_00359 Ps_contig_mira-and-tgicl-ass_franssen_3914
SEQUENCE=TAAACAGCTATGTAACCGATCCAGTTAGACTCACGGTCCCAACTCTCACGGGAAAGCGAGTGAAGCAAGAAGGCTTCAGGAACAGAGACACGTGCAGTAGCGTAAAGGAAAGAAACAACAGTGTAGTGTGTTGGGTTTTCAAGCATGACTTTGTTAAAGAACGTGGATTTTCCGTAACGGCTTGAACCGCAGTAGATAGAGTGTTGGTCGTTGTTGAAAGCATCGTAGGTTGTTTGTATGAAGTCACCGCAACGGAGTATGAGGTTGCGGAGGTTGAGGTCTAGTGGGTTTAATAGTGTTTCCCAGTTGTTGCTTCCTAGGAGTTGGTTCCATGTGTCTTGGTTTGTTGTTGTTGTTGATGTTGTTGATAATGTTGGTGGTGGTGGTGTTGTTTCTGTGTCTGTTGCCATGTTTATGGGACTTTTTTTGTGTGATAATCCCATGTTATAGGGAGTGTGTCTGTCTGTTTTTTTGAGGGAGTGGATATATATTGTGAGGAGTTGATGAGGGAAGAGAAGTGTGTGGTGAGA
TARGET=375,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2024
PRIMER_LEFT_PENALTY=0.121635
PRIMER_RIGHT_PENALTY=0.080808
PRIMER_LEFT_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT=152,22
PRIMER_RIGHT=411,22
PRIMER_LEFT_TM=59.878
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.2374
PRIMER_LEFT_1_PENALTY=0.121635
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=GTTAAAGAACGTGGATTTTCCG
PRIMER_RIGHT_1_SEQUENCE=AAAGTCCCATAAACATGGCAAC
PRIMER_LEFT_1=152,22
PRIMER_RIGHT_1=423,22
PRIMER_LEFT_1_TM=59.878
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.2454
PRIMER_LEFT_2_PENALTY=0.164560
PRIMER_RIGHT_2_PENALTY=0.080808
PRIMER_LEFT_2_SEQUENCE=GTTGTTGCTTCCTAGGAGTTGG
PRIMER_RIGHT_2_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT_2=305,22
PRIMER_RIGHT_2=411,22
PRIMER_LEFT_2_TM=60.165
PRIMER_RIGHT_2_TM=60.081
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=107
PRIMER_PAIR_PENALTY_3=0.2454
PRIMER_LEFT_3_PENALTY=0.164560
PRIMER_RIGHT_3_PENALTY=0.080808
PRIMER_LEFT_3_SEQUENCE=TTGTTGCTTCCTAGGAGTTGGT
PRIMER_RIGHT_3_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT_3=306,22
PRIMER_RIGHT_3=411,22
PRIMER_LEFT_3_TM=60.165
PRIMER_RIGHT_3_TM=60.081
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.2454
PRIMER_LEFT_4_PENALTY=0.164560
PRIMER_RIGHT_4_PENALTY=0.080808
PRIMER_LEFT_4_SEQUENCE=TGTTGCTTCCTAGGAGTTGGTT
PRIMER_RIGHT_4_SEQUENCE=ACATGGCAACAGACACAGAAAC
PRIMER_LEFT_4=307,22
PRIMER_RIGHT_4=411,22
PRIMER_LEFT_4_TM=60.165
PRIMER_RIGHT_4_TM=60.081
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00360 Ps_contig_mira-and-tgicl-ass_franssen_3934
SEQUENCE=ATCCCTACCTTAGGGTCCGAGTAACTTTACCTTTCCGTTGGTTCCTAGACTNCGGGCGNGTGGCTTCGTACTTGGCCCTCGTCCGTCCTTGTACATGTGCGCAATTTCTAGGCACAAAGTGGATCGTCAGGGTTGGGGTCCGGTCAACAATGAGCAAAATTGACAAAAAGGAACCTTGGAAATGGTTAGTGCTGGACTCCACTGTTCTTTGAGAATGTCGAGACATATGCTACCGTTGCTGTTGATGTTTGGGTGGAATACCTTGGTCCTAAAAGCAACCTTTGGTGGCTTGAAAGGATAGTCAGGAGGAAAATGTATCGAAACCAGGAAAACACCGCCTGTATACGGACTATCAGACGGTCCCATGATCGTGGCTTGCCAGTGGAACATGTCCTCAGCCACAGGACCTGCACTGCATGACGTTGGAGGATCCTTCTGCAAATCCTTGAGCTCCTTCAAGATACGTTTCGAAGCCATGATAATGGAAACTGAGTCGGGAGCGTGGAAGAAGAACGAATGATTGGGAATGAGGGTTGAAGAAGAAGAAAAGGAGAGATTTAGAAAAAATCGGAAAGTATTGAGTTGAATGTTCCCGCGTACTCTGCGTTGA
TARGET=536,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7376
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.730647
PRIMER_LEFT_SEQUENCE=AGATACGTTTCGAAGCCATGAT
PRIMER_RIGHT_SEQUENCE=GCGGGAACATTCAACTCAATAC
PRIMER_LEFT=458,22
PRIMER_RIGHT=595,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=60.731
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=138
PRIMER_PAIR_PENALTY_1=0.9145
PRIMER_LEFT_1_PENALTY=0.183834
PRIMER_RIGHT_1_PENALTY=0.730647
PRIMER_LEFT_1_SEQUENCE=GGAAAATGTATCGAAACCAGGA
PRIMER_RIGHT_1_SEQUENCE=GCGGGAACATTCAACTCAATAC
PRIMER_LEFT_1=307,22
PRIMER_RIGHT_1=595,22
PRIMER_LEFT_1_TM=60.184
PRIMER_RIGHT_1_TM=60.731
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=289
PRIMER_PAIR_PENALTY_2=0.9384
PRIMER_LEFT_2_PENALTY=0.207756
PRIMER_RIGHT_2_PENALTY=0.730647
PRIMER_LEFT_2_SEQUENCE=GATCCTTCTGCAAATCCTTGAG
PRIMER_RIGHT_2_SEQUENCE=GCGGGAACATTCAACTCAATAC
PRIMER_LEFT_2=428,22
PRIMER_RIGHT_2=595,22
PRIMER_LEFT_2_TM=60.208
PRIMER_RIGHT_2_TM=60.731
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.9502
PRIMER_LEFT_3_PENALTY=0.219551
PRIMER_RIGHT_3_PENALTY=0.730647
PRIMER_LEFT_3_SEQUENCE=GACTATCAGACGGTCCCATGAT
PRIMER_RIGHT_3_SEQUENCE=GCGGGAACATTCAACTCAATAC
PRIMER_LEFT_3=347,22
PRIMER_RIGHT_3=595,22
PRIMER_LEFT_3_TM=60.220
PRIMER_RIGHT_3_TM=60.731
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.5000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.9840
PRIMER_LEFT_4_PENALTY=0.253332
PRIMER_RIGHT_4_PENALTY=0.730647
PRIMER_LEFT_4_SEQUENCE=GTATCGAAACCAGGAAAACACC
PRIMER_RIGHT_4_SEQUENCE=GCGGGAACATTCAACTCAATAC
PRIMER_LEFT_4=314,22
PRIMER_RIGHT_4=595,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=60.731
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_00361 Ps_contig_mira-and-tgicl-ass_franssen_3937
SEQUENCE=AAAGAAAGGTTTCCCTTTCTTTTGGATTGGAAGCTTCACTGGAAGGCTTAGAGCCAGGGTATTTCCATTTCCAACGGCAGGCACGCTCACAACACACACACACTCGCACTTTTGAATCTGATTATGTTTTTTCTTTCACTATTGTACA
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00362 Ps_contig_mira-and-tgicl-ass_franssen_3944
SEQUENCE=CGTNTGGCAGTGACACCAAACCATCACGTTGCTTCTATCGTGGCTGCTGCATTTTATGCAATTTGGAACCNCTTTTCAGGGTTCTTCTTACCTTTTGATAACAGAGCATTCCAGTATGGTGGAGATGGTACTACTGGGCATGTCCAGTTGCATGGACCATCTACGGATTGGTTGCATCTCAATTTGGAGACATAACTACTCGTATGACTAGTGAAAGCGGAAAGGAGGTGAAATTTTTCCTTGAAGACTATTTTGGTATCAAACATGACTTCATAGGAGAGTGCGCAATTGTGGTTGCCGGTATTGCTGTTCTCTTTGCATTTATTTTTGCTGTTGCAATCAAGTCCTTTAACTTTCAAAAGAGATAGATTGTACTCTCTCTCTTTCTCCAACAAGAATTTCTTTATTTATTTTTTACTTTTCCCCTCCCCCTCTACTTTTCCTTGCTTGTATAATCTTGTATCTTGTTATTTCATACTAGTTAGTTTTTAAGGTAATTTACTTTTCCCCTTTGTTTGACACTAA
TARGET=375,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5638
PRIMER_LEFT_PENALTY=0.102129
PRIMER_RIGHT_PENALTY=0.461713
PRIMER_LEFT_SEQUENCE=CATGACTTCATAGGAGAGTGCG
PRIMER_RIGHT_SEQUENCE=AGTGTCAAACAAAGGGGAAAAG
PRIMER_LEFT=263,22
PRIMER_RIGHT=522,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=59.538
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.6912
PRIMER_LEFT_1_PENALTY=0.102129
PRIMER_RIGHT_1_PENALTY=0.589035
PRIMER_LEFT_1_SEQUENCE=CATGACTTCATAGGAGAGTGCG
PRIMER_RIGHT_1_SEQUENCE=GTGTCAAACAAAGGGGAAAAGT
PRIMER_LEFT_1=263,22
PRIMER_RIGHT_1=521,22
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=59.411
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=1.1450
PRIMER_LEFT_2_PENALTY=0.102129
PRIMER_RIGHT_2_PENALTY=1.042913
PRIMER_LEFT_2_SEQUENCE=CATGACTTCATAGGAGAGTGCG
PRIMER_RIGHT_2_SEQUENCE=ACAAGCAAGGAAAAGTAGAGGG
PRIMER_LEFT_2=263,22
PRIMER_RIGHT_2=450,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=58.957
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=1.1542
PRIMER_LEFT_3_PENALTY=0.102129
PRIMER_RIGHT_3_PENALTY=1.052069
PRIMER_LEFT_3_SEQUENCE=CATGACTTCATAGGAGAGTGCG
PRIMER_RIGHT_3_SEQUENCE=AAGGAAAAGTAGAGGGGGAGG
PRIMER_LEFT_3=263,22
PRIMER_RIGHT_3=444,21
PRIMER_LEFT_3_TM=59.898
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=1.2595
PRIMER_LEFT_4_PENALTY=0.216588
PRIMER_RIGHT_4_PENALTY=1.042913
PRIMER_LEFT_4_SEQUENCE=ATGACTAGTGAAAGCGGAAAGG
PRIMER_RIGHT_4_SEQUENCE=ACAAGCAAGGAAAAGTAGAGGG
PRIMER_LEFT_4=203,22
PRIMER_RIGHT_4=450,22
PRIMER_LEFT_4_TM=59.783
PRIMER_RIGHT_4_TM=58.957
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_00363 Ps_contig_mira-and-tgicl-ass_franssen_3964
SEQUENCE=GATCAAGAAACAAAGTAAAGGTGTGAACGTGAACTGAAAATGATGAAGAAGAATTCAGTGGTTTCTATGATGCTTTTAGTATTTCTTATAGTATTTTCTCAAATGGATAACGTTGTTCCAGATGCTTCTGATTGCTTAGATGGTTGCTCCACTGCATGCGTTTCAAGAGACTGTTTGTTACTCTTCTCAACCTTTTATTTTACTTATACTTAATTAATTAATTTTCTTCTTCTATGTTTTCTATTTTCTTATTTCTTACTACTAATTAAAATTTAT
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00364 Ps_contig_mira-and-tgicl-ass_franssen_3969
SEQUENCE=TTGCTGCATGTCACATAAATTTTAATTTAGTAGTAAGAAAATGAAAATAAAAAACATAGAAGAAGAAAATTAATTAATTAATTAATTAATTAATTAAGTATAAATAAAAGGTTGAGAAGAGTAACAAACAG
TARGET=68,28
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00365 Ps_contig_mira-and-tgicl-ass_franssen_3975
SEQUENCE=GCCAGCATAATCCACCAGCTTTAAATCGTCCATTATAGTGTATTCTAATTCAAATTAACWCCACCACCTTCACATTGAGTACATAATATTGCACCATTTCCATCACAAATACAGGAACAGAACAATGCTCCTAACTTTTGTTAGCTGGAGTACTATCTCCTGTTGTAGCCTTAACTTCCAAAGACTCAAAGTTTCTAACTTTAGAATTCAGACAAACGTCCTTCTTCTAAAAAGAACCTTTCCGGGTCCGAACCGAAACTTAACCCGTATAAAGAAGGTTCCGTACGGTTGGTTGGTTTTGGTTAAAGG
TARGET=287,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00366 Ps_contig_mira-and-tgicl-ass_franssen_3983
SEQUENCE=CCGTAATAAATNAAAAGGACTCTGAGGCAATTGGGCTAGGTAAAATTATAACTCAAAATACATTTTTAAACTATTATGGTTATAMAAGTAAAGAACATCTTATATTAAACTATTATGGTTAATTTGTAAATTACAGCGCCAAGGGCAGTGGAAAAGTGCAAGAACAGAAGCTGAAAACCTCGTTATCATGTTCCGACAAAAATCCTCTTGTTGTTGATCATGTATTGGCGTTCCAAGGAAGCAAATTGGGCTCGAGTAAGATCGGGGCTAATATAGGAATAATAAGAGTTCAATTATTATTATTATTATTATTGTTATGTGTCTGTGTTATTTCTTTTGTATTTTTTTTCTTCTTATATGTGTATTATATATGAATGAGAAATTAGGTGGAAATTCGTTAGGGACAAGAAATAATATATAAGATGTTTATATGACCCACTTTTTGTTTTCTAAGGAAAAAAAAATGTGACTTGTCTATTGCCAGCGTGTGCATGACTTATAATAAATGTCAATTTTCCAGACTATMAAACT
TARGET=293,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00367 Ps_contig_mira-and-tgicl-ass_franssen_4000
SEQUENCE=CTAGTGAAAAGCAGTTACATAATTTTAAGCAAATAAAGGAAGCGTTGGATTTGGATTACATTTTGGTACATAATATAACATTTATAAAGATAAAAATAAAATTAAAAACAGGTCACCAGTGCTAGATTCATCATTCATGTTATGGTAACAATCTCAGCATCTGATTCAACCAATTTAGTTCTTTCTTTTTTTGAAAATAGTACTAGTAATAATAATAATAATAATAATAAATTAAAAAAAAAACATGAACTAAGAAGAAAGTGGCCTTGGTGGAGGAGGAAGTAGTTGATGGAGTATTTGGAGGACTCTGCGTTGACTACCACTGCTT
TARGET=207,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3365
PRIMER_LEFT_PENALTY=0.312496
PRIMER_RIGHT_PENALTY=0.023981
PRIMER_LEFT_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_RIGHT_SEQUENCE=CAGTGGTAGTCAACGCAGAGTC
PRIMER_LEFT=108,22
PRIMER_RIGHT=324,22
PRIMER_LEFT_TM=60.312
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.5336
PRIMER_LEFT_1_PENALTY=0.312496
PRIMER_RIGHT_1_PENALTY=0.221115
PRIMER_LEFT_1_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_RIGHT_1_SEQUENCE=ACGCAGAGTCCTCCAAATACTC
PRIMER_LEFT_1=108,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=59.779
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.7364
PRIMER_LEFT_2_PENALTY=0.312496
PRIMER_RIGHT_2_PENALTY=0.423908
PRIMER_LEFT_2_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_RIGHT_2_SEQUENCE=TCCATCAACTACTTCCTCCTCC
PRIMER_LEFT_2=108,22
PRIMER_RIGHT_2=292,22
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=59.576
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.7997
PRIMER_LEFT_3_PENALTY=0.775718
PRIMER_RIGHT_3_PENALTY=0.023981
PRIMER_LEFT_3_SEQUENCE=TCTCAGCATCTGATTCAACCAA
PRIMER_RIGHT_3_SEQUENCE=CAGTGGTAGTCAACGCAGAGTC
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=324,22
PRIMER_LEFT_3_TM=60.776
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.8001
PRIMER_LEFT_4_PENALTY=0.312496
PRIMER_RIGHT_4_PENALTY=0.487583
PRIMER_LEFT_4_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_RIGHT_4_SEQUENCE=CCATCAACTACTTCCTCCTCCA
PRIMER_LEFT_4=108,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=60.488
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_00368 Ps_contig_mira-and-tgicl-ass_franssen_4002
SEQUENCE=TCCTCCAAATCTCCATCAACTACTTCCTCCTCCACCAAGGCCACTTTCTTCTTAGTTCATGTTTTTTTTTTAATTATTATTATTATTATTACTAGTACTATTTTCAAAAAARRAAGAACTAAATTGGTTGAATACAGATGCTGAGATTGTTACCATAACATGAATGATGAATCTAGCACTGGTNACCTTGTTTTTTAATTTTATTTTTATCTTTATAAATGTTATATTATGTACCAAAGTGTAANCCAAATCCAACGCTTTCTTTATTTGTTTAAAATTNTGTAACTGTCTTTT
TARGET=73,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00369 Ps_contig_mira-and-tgicl-ass_franssen_4008
SEQUENCE=GACGTTCCGGTGAATATGCGGGAACTAGTTGACTGCATGAGAGATGTTCGAATTGCGGATTCGGGTCGGGTCGGGTGTCCGCAGGGTGGGTTTTTAGTCTTCCGGCAAGCTATGGAAGGGGTTAGTATGGAGAGGGTGGAATCTGGAAGAGATCTTAGAGCCAAAATCTATGGAAAATTCAGTAAGGTGAACTTCAACGACGGTTGTTGTTTCGGTTCCTGTTCCTGTATTTTGGATTGGGTTTCGGAACTGGTTAACTGGTAATTTTRGGGATTGGAGGTTGAGGAGTATGATTYYGCTTTGTATATAGGATGAATGATGACAAAAAGGAAGAAGTAAATTCGTTTTGGTGCTAAGTTAAGGTTTGGAAGGG
TARGET=61,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00370 Ps_contig_mira-and-tgicl-ass_franssen_4010
SEQUENCE=GACGTCCACAATAAACTAGTTACATTGACAATCTGTCACCATGGCGGCTCATATTAAACACCAATCCCAATCCACTCTTCTCTCTCAATCTCACCCTCTTATAATTCCCTCACCACCACCACCATTTCTCCGATCACCCTTCTCTTCACTTTCCGGCGAGCCAAATCTTTTTATCACCATTTCCTACACTAAGTAACAACTCAACAAAAAAAACAAATCATGTACAAACGCAGATTAACAAACAAACTAAACCATTNTA
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2570
PRIMER_LEFT_PENALTY=0.226866
PRIMER_RIGHT_PENALTY=0.030114
PRIMER_LEFT_SEQUENCE=GCGGCTCATATTAAACACCAAT
PRIMER_RIGHT_SEQUENCE=GGTGATAAAAAGATTTGGCTCG
PRIMER_LEFT=43,22
PRIMER_RIGHT=177,22
PRIMER_LEFT_TM=60.227
PRIMER_RIGHT_TM=59.970
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=0.2846
PRIMER_LEFT_1_PENALTY=0.182682
PRIMER_RIGHT_1_PENALTY=0.101938
PRIMER_LEFT_1_SEQUENCE=TCCCAATCCACTCTTCTCTCTC
PRIMER_RIGHT_1_SEQUENCE=GTGATAAAAAGATTTGGCTCGC
PRIMER_LEFT_1=64,22
PRIMER_RIGHT_1=176,22
PRIMER_LEFT_1_TM=59.817
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=0.3288
PRIMER_LEFT_2_PENALTY=0.226866
PRIMER_RIGHT_2_PENALTY=0.101938
PRIMER_LEFT_2_SEQUENCE=GCGGCTCATATTAAACACCAAT
PRIMER_RIGHT_2_SEQUENCE=GTGATAAAAAGATTTGGCTCGC
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=176,22
PRIMER_LEFT_2_TM=60.227
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=0.4540
PRIMER_LEFT_3_PENALTY=0.423908
PRIMER_RIGHT_3_PENALTY=0.030114
PRIMER_LEFT_3_SEQUENCE=CAATCCCAATCCACTCTTCTCT
PRIMER_RIGHT_3_SEQUENCE=GGTGATAAAAAGATTTGGCTCG
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=177,22
PRIMER_LEFT_3_TM=59.576
PRIMER_RIGHT_3_TM=59.970
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=117
PRIMER_PAIR_PENALTY_4=0.4757
PRIMER_LEFT_4_PENALTY=0.226866
PRIMER_RIGHT_4_PENALTY=0.248820
PRIMER_LEFT_4_SEQUENCE=GCGGCTCATATTAAACACCAAT
PRIMER_RIGHT_4_SEQUENCE=AAAGTGAAGAGAAGGGTGATCG
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=151,22
PRIMER_LEFT_4_TM=60.227
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_00371 Ps_contig_mira-and-tgicl-ass_franssen_4013
SEQUENCE=GTACTTGACCCGATAGCTCCATACAAATTACAACACCTCTCTCTCTCTCTCTTCCATTCACGCTTCTCAACAAACTCTCTCTCTCCCATGGCGACTTCTTTCGCGCCATTATCCATTTCAGTTGGTGGATCTCATCTCAAGTCATCTCTGGTTCGACCTAACCTTGCTGTTCAAAGGAAATCAAACATTCTGACAAGAAAGAACCTCACTTCACCACTTCGTGCAGAATACCGTGATAACAGAGGAGGTGGAGGTGGGGAATTGCTTA
TARGET=37,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4966
PRIMER_LEFT_PENALTY=0.491057
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=ACTTGACCCGATAGCTCCATAC
PRIMER_RIGHT_SEQUENCE=CACCTCCACCTCCTCTGTTATC
PRIMER_LEFT=2,22
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=59.509
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.4966
PRIMER_LEFT_1_PENALTY=0.491057
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=GTACTTGACCCGATAGCTCCAT
PRIMER_RIGHT_1_SEQUENCE=CACCTCCACCTCCTCTGTTATC
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=255,22
PRIMER_LEFT_1_TM=59.509
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.5016
PRIMER_LEFT_2_PENALTY=0.491057
PRIMER_RIGHT_2_PENALTY=0.010545
PRIMER_LEFT_2_SEQUENCE=ACTTGACCCGATAGCTCCATAC
PRIMER_RIGHT_2_SEQUENCE=TCGAACCAGAGATGACTTGAGA
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=155,22
PRIMER_LEFT_2_TM=59.509
PRIMER_RIGHT_2_TM=59.989
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=0.5016
PRIMER_LEFT_3_PENALTY=0.491057
PRIMER_RIGHT_3_PENALTY=0.010545
PRIMER_LEFT_3_SEQUENCE=GTACTTGACCCGATAGCTCCAT
PRIMER_RIGHT_3_SEQUENCE=TCGAACCAGAGATGACTTGAGA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=155,22
PRIMER_LEFT_3_TM=59.509
PRIMER_RIGHT_3_TM=59.989
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.5729
PRIMER_LEFT_4_PENALTY=0.491057
PRIMER_RIGHT_4_PENALTY=0.081801
PRIMER_LEFT_4_SEQUENCE=ACTTGACCCGATAGCTCCATAC
PRIMER_RIGHT_4_SEQUENCE=ACCAACTGAAATGGATAATGGC
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=125,22
PRIMER_LEFT_4_TM=59.509
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_00372 Ps_contig_mira-and-tgicl-ass_franssen_4013
SEQUENCE=GTACTTGACCCGATAGCTCCATACAAATTACAACACCTCTCTCTCTCTCTCTTCCATTCACGCTTCTCAACAAACTCTCTCTCTCCCATGGCGACTTCTTTCGCGCCATTATCCATTTCAGTTGGTGGATCTCATCTCAAGTCATCTCTGGTTCGACCTAACCTTGCTGTTCAAAGGAAATCAAACATTCTGACAAGAAAGAACCTCACTTCACCACTTCGTGCAGAATACCGTGATAACAGAGGAGGTGGAGGTGGGGAATTGCTTA
TARGET=75,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1587
PRIMER_LEFT_PENALTY=0.153161
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=CTCTCTCTCTTCCATTCACGCT
PRIMER_RIGHT_SEQUENCE=CACCTCCACCTCCTCTGTTATC
PRIMER_LEFT=42,22
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=60.153
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=214
PRIMER_PAIR_PENALTY_1=0.4629
PRIMER_LEFT_1_PENALTY=0.153161
PRIMER_RIGHT_1_PENALTY=0.309780
PRIMER_LEFT_1_SEQUENCE=CTCTCTCTCTTCCATTCACGCT
PRIMER_RIGHT_1_SEQUENCE=CCTTTGAACAGCAAGGTTAGGT
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=176,22
PRIMER_LEFT_1_TM=60.153
PRIMER_RIGHT_1_TM=59.690
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=0.4966
PRIMER_LEFT_2_PENALTY=0.491057
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=ACTTGACCCGATAGCTCCATAC
PRIMER_RIGHT_2_SEQUENCE=CACCTCCACCTCCTCTGTTATC
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=59.509
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=0.4966
PRIMER_LEFT_3_PENALTY=0.491057
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=GTACTTGACCCGATAGCTCCAT
PRIMER_RIGHT_3_SEQUENCE=CACCTCCACCTCCTCTGTTATC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=59.509
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.5016
PRIMER_LEFT_4_PENALTY=0.491057
PRIMER_RIGHT_4_PENALTY=0.010545
PRIMER_LEFT_4_SEQUENCE=ACTTGACCCGATAGCTCCATAC
PRIMER_RIGHT_4_SEQUENCE=TCGAACCAGAGATGACTTGAGA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=155,22
PRIMER_LEFT_4_TM=59.509
PRIMER_RIGHT_4_TM=59.989
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_00373 Ps_contig_mira-and-tgicl-ass_franssen_4014
SEQUENCE=GTATACAACGCAGAGTACGCGGAGTGACCCGATAGCTCCATACAAATTACAACACCTCTCTCTCTCTCTCTCTCTTCCATTCACGCTTCTACAAACAAACTCTCTCTCCTCCCATGGCGACTTCTTCGCGCCATTATCCTATTTCTAGGTTGGGTGGATCTCATCTACAAGTCATCTCGTTGGGTTCCGACCCTTAACCTTG
TARGET=56,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1808
PRIMER_LEFT_PENALTY=0.010294
PRIMER_RIGHT_PENALTY=0.170538
PRIMER_LEFT_SEQUENCE=GAGTGACCCGATAGCTCCATAC
PRIMER_RIGHT_SEQUENCE=TCCACCCAACCTAGAAATAGGA
PRIMER_LEFT=21,22
PRIMER_RIGHT=157,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=59.829
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=0.1836
PRIMER_LEFT_1_PENALTY=0.010294
PRIMER_RIGHT_1_PENALTY=0.173354
PRIMER_LEFT_1_SEQUENCE=GAGTGACCCGATAGCTCCATAC
PRIMER_RIGHT_1_SEQUENCE=ATGAGATCCACCCAACCTAGAA
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=163,22
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=59.827
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.3378
PRIMER_LEFT_2_PENALTY=0.010294
PRIMER_RIGHT_2_PENALTY=0.327533
PRIMER_LEFT_2_SEQUENCE=GAGTGACCCGATAGCTCCATAC
PRIMER_RIGHT_2_SEQUENCE=GATGAGATCCACCCAACCTAGA
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=164,22
PRIMER_LEFT_2_TM=59.990
PRIMER_RIGHT_2_TM=60.328
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=0.6833
PRIMER_LEFT_3_PENALTY=0.010294
PRIMER_RIGHT_3_PENALTY=0.672968
PRIMER_LEFT_3_SEQUENCE=GAGTGACCCGATAGCTCCATAC
PRIMER_RIGHT_3_SEQUENCE=GTAGATGAGATCCACCCAACCT
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=167,22
PRIMER_LEFT_3_TM=59.990
PRIMER_RIGHT_3_TM=59.327
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=0.8118
PRIMER_LEFT_4_PENALTY=0.641309
PRIMER_RIGHT_4_PENALTY=0.170538
PRIMER_LEFT_4_SEQUENCE=GACCCGATAGCTCCATACAAAT
PRIMER_RIGHT_4_SEQUENCE=TCCACCCAACCTAGAAATAGGA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=157,22
PRIMER_LEFT_4_TM=59.359
PRIMER_RIGHT_4_TM=59.829
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_00374 Ps_contig_mira-and-tgicl-ass_franssen_4039
SEQUENCE=GTATTTGGTAATAGNTTCNCAAGCCAGCTATAACAGAAATCAAATAGTTGGTTGGAGGTTTTGTCAAGAGATWCATGAAACAAGCAAACTTACTCTATCAGTAATTGATTTCAGATCCATTTGGTCGGTCAACGCTTGTTTAGAAAGAGCCCCTGCCTTGTCAATCTTGTTCCCCAACACCAATAGAGGGATACCACTCAATGATGGTTTGCTCAGCAAATCATGAAGTTCACTTCTTGATATGCTAAGATTATCTGGATCAGCAGCATCAACAACATAAACAATAGCAGAAACCGCACGGCAGTAGCGTTCCCACATGCTACGGAACCTAGGTTGCCCTCCAAGATCCCATAGCTTAATTGTAACATTGCCTTTTGTTACTTTCCTCATATTGAATCCCACGGTAGGAATCATGTCCTCGCTGTATCCACCGGTAGCAACAACATTTACGAAGGGAAGTCTTTCCAGCATTCTGCAGTCCTATAAGAGATAACTCCATTTCCTGCTTAGAAGAAAGGCTGACGTAACCAATTACAAAAAGCCTTCCCACAACTCCCTATTTCGTTCCTTTTTACCCGTAACTTTAATTTCCTTTTCGTTCCTTCCAACCAACCAAACCCTCCCTTCCCTCTTCCCG
TARGET=605,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00375 Ps_contig_mira-and-tgicl-ass_franssen_4052
SEQUENCE=GGGGKAGAATCGTACCTTCAAAATTTTCCCAAAATGAGCGATTGTGGTTGTGAGACCTCACAGACCAACTGAAGCCGTCATGGAGGTTGCGCCGGTGAGGAGCGTTTGAAGGTTGATTGTGGCCGGCGGAGAAATTGTGGTTTTGTGAGGGTGCTCGTTGGAGGTAGAAAATGAGGGTGTGGAACGTGTGGGTTCGCGGTCGAAGGAGGAGAAGAGGTGCGTGTTTGGAGGTTTGGAAGGATTCGTTGGAAGAAGAAGAAGATGAAGTTGAGTTTGATGCGTCCCGTCTTCAGATAAGCCTCCTTCTTCTGTTTCGGTTTTTTTTTAGGGCTTTTTGAAAATATTGCTCTTTGTTGTTGTTCTTGCTGTGCGTTTTCTGTTGTGTACTGGTTTAGGGTTTTGAGAAAAATATCTTTGTTGTGTATCCTCTTGCTGTTAACTTTTTAGGGCTCTTAGAAAAAATTGATTTGTTCTTGCTGTTGTGTTGTTTAGGGTTTTAGAAAAAATCTCTGTGTTGTTGCTGTTGTTCTTTTGGTTTTTTTTTAGAAATAATTTGTTACTGTGTTGTGTTCCTGTTAACCTCTCCTAATCCATTTTTGAAGACAAGATTCAGACTTTAAAAACTCTTTGCTTTTTGAATTTCCCATCCCAGATTATGTGGGATTTGGTTTATTCTGTTTTAAGCTAATGCCAAAAGTGCTTTTTGGATGTTTATGTTAACCTTATGGATAAAGCTTCAAACTGAGTAATTTAAGTTTAAAGTTATTTTCAGTTTTTAAGCGCTTTTGAAAAGTGCT
TARGET=249,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00376 Ps_contig_mira-and-tgicl-ass_franssen_4053
SEQUENCE=GCCTGCATAACCACCAGCACCACCACTGCCCCCATAACCTCCAAAACCAAACTCTTCAGAACTTTTCAAAAACCTGTTGAATAACAACCCCCTTTTTCAATCAATCAACATCAACATCACTTCACCTGACAAAAAACCAATTACAAAAAAAACAGCTTATTCAAAAAGCACTTTTC
TARGET=97,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5309
PRIMER_LEFT_PENALTY=0.717980
PRIMER_RIGHT_PENALTY=0.812957
PRIMER_LEFT_SEQUENCE=CCATAACCTCCAAAACCAAACT
PRIMER_RIGHT_SEQUENCE=TTGTCAGGTGAAGTGATGTTGA
PRIMER_LEFT=31,22
PRIMER_RIGHT=131,22
PRIMER_LEFT_TM=59.282
PRIMER_RIGHT_TM=59.187
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=2.0433
PRIMER_LEFT_1_PENALTY=0.717980
PRIMER_RIGHT_1_PENALTY=1.325343
PRIMER_LEFT_1_SEQUENCE=CCATAACCTCCAAAACCAAACT
PRIMER_RIGHT_1_SEQUENCE=TTTGTCAGGTGAAGTGATGTTG
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=132,22
PRIMER_LEFT_1_TM=59.282
PRIMER_RIGHT_1_TM=58.675
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=2.4367
PRIMER_LEFT_2_PENALTY=1.623715
PRIMER_RIGHT_2_PENALTY=0.812957
PRIMER_LEFT_2_SEQUENCE=CCCATAACCTCCAAAACCAAAC
PRIMER_RIGHT_2_SEQUENCE=TTGTCAGGTGAAGTGATGTTGA
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=131,22
PRIMER_LEFT_2_TM=61.624
PRIMER_RIGHT_2_TM=59.187
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=102
PRIMER_PAIR_PENALTY_3=2.6598
PRIMER_LEFT_3_PENALTY=0.717980
PRIMER_RIGHT_3_PENALTY=1.941852
PRIMER_LEFT_3_SEQUENCE=CCATAACCTCCAAAACCAAACT
PRIMER_RIGHT_3_SEQUENCE=TGTCAGGTGAAGTGATGTTGAT
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=130,22
PRIMER_LEFT_3_TM=59.282
PRIMER_RIGHT_3_TM=58.058
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=2.7279
PRIMER_LEFT_4_PENALTY=1.914963
PRIMER_RIGHT_4_PENALTY=0.812957
PRIMER_LEFT_4_SEQUENCE=CCCATAACCTCCAAAACCAAA
PRIMER_RIGHT_4_SEQUENCE=TTGTCAGGTGAAGTGATGTTGA
PRIMER_LEFT_4=30,21
PRIMER_RIGHT_4=131,22
PRIMER_LEFT_4_TM=60.915
PRIMER_RIGHT_4_TM=59.187
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=102
=
PRIMER_SEQUENCE_ID=SSR_00377 Ps_contig_mira-and-tgicl-ass_franssen_4057
SEQUENCE=CTCTYTTTGGAAGTTCCCAATTCAAAAACCAACTCTYCAGAACTTTTCAAAAACCTGTTGAATAACAACCCCCTTTTTCAATCAATCAACATCAACATCACTTCACCTGCAAAAACCAATTCAAAATCAAAAAACAGCTTATTCAAAAAGCACTTTTCAAAAGCGCTTAAAAAACTGAAAAATAACTAAACAAATTACTCAGTTTGAAGCTTTATCCATAAGGTTAACATAAACATCCAAAAAGCACTTTTGGCATTAGCTTAAAACAGAATAAACCAAA
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00378 Ps_contig_mira-and-tgicl-ass_franssen_4061
SEQUENCE=GATTCACGTTCATCTCAATGTCAGCTCTACACACTCCCTTCACTCACATTCCCTACAATCATTATTCAATCTTCACAAATAGGCTTTATTACGCTTCTACATTTCCCCCTCCAATAACACTCCACCACCTCCCCAAATTAACATTCTCTGCTTCTTCTTCTTCTGTTCTCCCTACTGTTGTTGATAATGGAAGCCACAACGTCGAACAAGATTCGAAGAAAGAGAATCAATTTGTGGATGAAAAAGAGATGACGAGAAGAATGAGAATAGGACTAGCTAACAAAGGAAAAGCACCTTGGAATAAGGGAAGGAAACACACTGCAG
TARGET=151,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0544
PRIMER_LEFT_PENALTY=0.050763
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=TCAGCTCTACACACTCCCTTCA
PRIMER_RIGHT_SEQUENCE=TGGCTTCCATTATCAACAACAG
PRIMER_LEFT=20,22
PRIMER_RIGHT=195,22
PRIMER_LEFT_TM=60.051
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.1612
PRIMER_LEFT_1_PENALTY=0.050763
PRIMER_RIGHT_1_PENALTY=0.110392
PRIMER_LEFT_1_SEQUENCE=TCAGCTCTACACACTCCCTTCA
PRIMER_RIGHT_1_SEQUENCE=CGTCATCTCTTTTTCATCCACA
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=253,22
PRIMER_LEFT_1_TM=60.051
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.1836
PRIMER_LEFT_2_PENALTY=0.050763
PRIMER_RIGHT_2_PENALTY=0.132857
PRIMER_LEFT_2_SEQUENCE=TCAGCTCTACACACTCCCTTCA
PRIMER_RIGHT_2_SEQUENCE=GTGGCTTCCATTATCAACAACA
PRIMER_LEFT_2=20,22
PRIMER_RIGHT_2=196,22
PRIMER_LEFT_2_TM=60.051
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=0.1836
PRIMER_LEFT_3_PENALTY=0.050763
PRIMER_RIGHT_3_PENALTY=0.132857
PRIMER_LEFT_3_SEQUENCE=TCAGCTCTACACACTCCCTTCA
PRIMER_RIGHT_3_SEQUENCE=GTTGTGGCTTCCATTATCAACA
PRIMER_LEFT_3=20,22
PRIMER_RIGHT_3=199,22
PRIMER_LEFT_3_TM=60.051
PRIMER_RIGHT_3_TM=59.867
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.1836
PRIMER_LEFT_4_PENALTY=0.050763
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_LEFT_4_SEQUENCE=TCAGCTCTACACACTCCCTTCA
PRIMER_RIGHT_4_SEQUENCE=TGTGGCTTCCATTATCAACAAC
PRIMER_LEFT_4=20,22
PRIMER_RIGHT_4=197,22
PRIMER_LEFT_4_TM=60.051
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_00379 Ps_contig_mira-and-tgicl-ass_franssen_4068
SEQUENCE=GAACASAACACTAAATTCCACTCAACCCTACTTTCTCTCTCTTCTCTCTCTCTCTCTCTACAATCTCAACAATGGTCGTTCGCTTCTTTCCTCGGAAGAGTCCTACTTAAACGCCTCCGTCTTCATCCTCTCTGCTTA
TARGET=43,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00380 Ps_contig_mira-and-tgicl-ass_franssen_4091
SEQUENCE=CTTCTTTGCAGCCACCTGCAATGCATATTTACAGTTCAAAAATTGACAAAAAGCTCCTGGCCAGTTAGCAAACTCCAACCACAACATAAACATGGAAGCATTTTGAATTTATTTATTTCAAACCTCTTCTCCAATAATAGTAATGTTTGGATGAGTTTGTAGAGCACATTCCAGTGTAATGTGGGAAGCTGCACGCCCCATAAGCCGCACAAAATGGTAATATTTTCCGGTTGATCGGGCATCTATCATAACGTTTCCTATCATTTCTGCATATATCTTGCAAGCAGTATCAAACCCAAAGCTTGTCGGAACTTCTTTACTTTTCAGATCGCCATCAATGGTTTTGGGGCATCCAAGCACCTGTGTTTTTAAATTTTTGTCTTCTGAAGTTCTCGGCAAGGAGGCATGCATTCGTGTTGGAATCATCTCCACCAATAACAACAAGCCCATCCAACTCTAGCTTCTTCGCTGTTTCCTCAGCTTGTTTAAACTGCTCTGGAGTTTCAATCTTGTCCCTCCCACTGCAAATCATGTCAAAACCACCCTGATTTCTATAAGGATAAATATACTCAGAATTGAGTTCAGTGTATTTGCACTTCATAATGCCGGCAGGACCACCTTTGAAACCATACAATGTGCTTCCACTTGCAAGTTGTTGAAGATAATCGTAAATTCCAGATATAACATTGTGACCTCCAGGAGCCTGTCCACCGGAGAGAACAACGCCAATCTTCAACTTCCGATGAGGAAGCGCATTGGTCGAATCAGCAGGCACCAAAGCAGCGGAAGGTTGTCCAAACAAAAATGGAAACAGTTTCGCGATCTCATCTGGATTTCCGGCGGCGGAGCTTTTAGGACCGTCGACAATGGCGAACGGTGTTTGGAGAACGGAAGGAAGACGGAGTTTGTGATCGACGCGGCTGTTTTGGACTTCGCTGTAGACGGAAGCGAAGCGAGTTGTGACGGTGGGAGTTACGTTTCCGTTACTTACGATGGAAGCTGGTGCCATTGTTTGTTGAGAGAGTGAGAGAGAGAGTGTGTGTGTTGTTGAGAAGTCCCCCCGTACTCTGCGTTGATAC
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3686
PRIMER_LEFT_PENALTY=0.095538
PRIMER_RIGHT_PENALTY=0.273036
PRIMER_LEFT_SEQUENCE=GCAAACTCCAACCACAACATAA
PRIMER_RIGHT_SEQUENCE=AACGTTATGATAGATGCCCGAT
PRIMER_LEFT=67,22
PRIMER_RIGHT=254,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=59.727
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=9.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.3896
PRIMER_LEFT_1_PENALTY=0.095538
PRIMER_RIGHT_1_PENALTY=0.294013
PRIMER_LEFT_1_SEQUENCE=GCAAACTCCAACCACAACATAA
PRIMER_RIGHT_1_SEQUENCE=GGAAACGTTATGATAGATGCCC
PRIMER_LEFT_1=67,22
PRIMER_RIGHT_1=257,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=59.706
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.4764
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.273036
PRIMER_LEFT_2_SEQUENCE=CAGTTAGCAAACTCCAACCACA
PRIMER_RIGHT_2_SEQUENCE=AACGTTATGATAGATGCCCGAT
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=254,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=59.727
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.4974
PRIMER_LEFT_3_PENALTY=0.203343
PRIMER_RIGHT_3_PENALTY=0.294013
PRIMER_LEFT_3_SEQUENCE=CAGTTAGCAAACTCCAACCACA
PRIMER_RIGHT_3_SEQUENCE=GGAAACGTTATGATAGATGCCC
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=60.203
PRIMER_RIGHT_3_TM=59.706
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=0.5461
PRIMER_LEFT_4_PENALTY=0.513718
PRIMER_RIGHT_4_PENALTY=0.032347
PRIMER_LEFT_4_SEQUENCE=CCAACCACAACATAAACATGGA
PRIMER_RIGHT_4_SEQUENCE=TAAAAACACAGGTGCTTGGATG
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=370,22
PRIMER_LEFT_4_TM=60.514
PRIMER_RIGHT_4_TM=60.032
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_00381 Ps_contig_mira-and-tgicl-ass_franssen_4091
SEQUENCE=CTTCTTTGCAGCCACCTGCAATGCATATTTACAGTTCAAAAATTGACAAAAAGCTCCTGGCCAGTTAGCAAACTCCAACCACAACATAAACATGGAAGCATTTTGAATTTATTTATTTCAAACCTCTTCTCCAATAATAGTAATGTTTGGATGAGTTTGTAGAGCACATTCCAGTGTAATGTGGGAAGCTGCACGCCCCATAAGCCGCACAAAATGGTAATATTTTCCGGTTGATCGGGCATCTATCATAACGTTTCCTATCATTTCTGCATATATCTTGCAAGCAGTATCAAACCCAAAGCTTGTCGGAACTTCTTTACTTTTCAGATCGCCATCAATGGTTTTGGGGCATCCAAGCACCTGTGTTTTTAAATTTTTGTCTTCTGAAGTTCTCGGCAAGGAGGCATGCATTCGTGTTGGAATCATCTCCACCAATAACAACAAGCCCATCCAACTCTAGCTTCTTCGCTGTTTCCTCAGCTTGTTTAAACTGCTCTGGAGTTTCAATCTTGTCCCTCCCACTGCAAATCATGTCAAAACCACCCTGATTTCTATAAGGATAAATATACTCAGAATTGAGTTCAGTGTATTTGCACTTCATAATGCCGGCAGGACCACCTTTGAAACCATACAATGTGCTTCCACTTGCAAGTTGTTGAAGATAATCGTAAATTCCAGATATAACATTGTGACCTCCAGGAGCCTGTCCACCGGAGAGAACAACGCCAATCTTCAACTTCCGATGAGGAAGCGCATTGGTCGAATCAGCAGGCACCAAAGCAGCGGAAGGTTGTCCAAACAAAAATGGAAACAGTTTCGCGATCTCATCTGGATTTCCGGCGGCGGAGCTTTTAGGACCGTCGACAATGGCGAACGGTGTTTGGAGAACGGAAGGAAGACGGAGTTTGTGATCGACGCGGCTGTTTTGGACTTCGCTGTAGACGGAAGCGAAGCGAGTTGTGACGGTGGGAGTTACGTTTCCGTTACTTACGATGGAAGCTGGTGCCATTGTTTGTTGAGAGAGTGAGAGAGAGAGTGTGTGTGTTGTTGAGAAGTCCCCCCGTACTCTGCGTTGATAC
TARGET=1026,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7462
PRIMER_LEFT_PENALTY=0.269585
PRIMER_RIGHT_PENALTY=0.476630
PRIMER_LEFT_SEQUENCE=AAACAGTTTCGCGATCTCATCT
PRIMER_RIGHT_SEQUENCE=GGGACTTCTCAACAACACACAC
PRIMER_LEFT=808,22
PRIMER_RIGHT=1058,22
PRIMER_LEFT_TM=60.270
PRIMER_RIGHT_TM=59.523
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.9195
PRIMER_LEFT_1_PENALTY=0.442900
PRIMER_RIGHT_1_PENALTY=0.476630
PRIMER_LEFT_1_SEQUENCE=CTGTTTTGGACTTCGCTGTAGA
PRIMER_RIGHT_1_SEQUENCE=GGGACTTCTCAACAACACACAC
PRIMER_LEFT_1=920,22
PRIMER_RIGHT_1=1058,22
PRIMER_LEFT_1_TM=59.557
PRIMER_RIGHT_1_TM=59.523
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=139
PRIMER_PAIR_PENALTY_2=1.0522
PRIMER_LEFT_2_PENALTY=0.575571
PRIMER_RIGHT_2_PENALTY=0.476630
PRIMER_LEFT_2_SEQUENCE=TGTTTTGGACTTCGCTGTAGAC
PRIMER_RIGHT_2_SEQUENCE=GGGACTTCTCAACAACACACAC
PRIMER_LEFT_2=921,22
PRIMER_RIGHT_2=1058,22
PRIMER_LEFT_2_TM=59.424
PRIMER_RIGHT_2_TM=59.523
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=138
PRIMER_PAIR_PENALTY_3=1.5937
PRIMER_LEFT_3_PENALTY=0.269585
PRIMER_RIGHT_3_PENALTY=1.324098
PRIMER_LEFT_3_SEQUENCE=AAACAGTTTCGCGATCTCATCT
PRIMER_RIGHT_3_SEQUENCE=GGACTTCTCAACAACACACACA
PRIMER_LEFT_3=808,22
PRIMER_RIGHT_3=1057,22
PRIMER_LEFT_3_TM=60.270
PRIMER_RIGHT_3_TM=58.676
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=1.6643
PRIMER_LEFT_4_PENALTY=1.187697
PRIMER_RIGHT_4_PENALTY=0.476630
PRIMER_LEFT_4_SEQUENCE=GTTTTGGACTTCGCTGTAGACG
PRIMER_RIGHT_4_SEQUENCE=GGGACTTCTCAACAACACACAC
PRIMER_LEFT_4=922,22
PRIMER_RIGHT_4=1058,22
PRIMER_LEFT_4_TM=61.188
PRIMER_RIGHT_4_TM=59.523
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_00382 Ps_contig_mira-and-tgicl-ass_franssen_4091
SEQUENCE=CTTCTTTGCAGCCACCTGCAATGCATATTTACAGTTCAAAAATTGACAAAAAGCTCCTGGCCAGTTAGCAAACTCCAACCACAACATAAACATGGAAGCATTTTGAATTTATTTATTTCAAACCTCTTCTCCAATAATAGTAATGTTTGGATGAGTTTGTAGAGCACATTCCAGTGTAATGTGGGAAGCTGCACGCCCCATAAGCCGCACAAAATGGTAATATTTTCCGGTTGATCGGGCATCTATCATAACGTTTCCTATCATTTCTGCATATATCTTGCAAGCAGTATCAAACCCAAAGCTTGTCGGAACTTCTTTACTTTTCAGATCGCCATCAATGGTTTTGGGGCATCCAAGCACCTGTGTTTTTAAATTTTTGTCTTCTGAAGTTCTCGGCAAGGAGGCATGCATTCGTGTTGGAATCATCTCCACCAATAACAACAAGCCCATCCAACTCTAGCTTCTTCGCTGTTTCCTCAGCTTGTTTAAACTGCTCTGGAGTTTCAATCTTGTCCCTCCCACTGCAAATCATGTCAAAACCACCCTGATTTCTATAAGGATAAATATACTCAGAATTGAGTTCAGTGTATTTGCACTTCATAATGCCGGCAGGACCACCTTTGAAACCATACAATGTGCTTCCACTTGCAAGTTGTTGAAGATAATCGTAAATTCCAGATATAACATTGTGACCTCCAGGAGCCTGTCCACCGGAGAGAACAACGCCAATCTTCAACTTCCGATGAGGAAGCGCATTGGTCGAATCAGCAGGCACCAAAGCAGCGGAAGGTTGTCCAAACAAAAATGGAAACAGTTTCGCGATCTCATCTGGATTTCCGGCGGCGGAGCTTTTAGGACCGTCGACAATGGCGAACGGTGTTTGGAGAACGGAAGGAAGACGGAGTTTGTGATCGACGCGGCTGTTTTGGACTTCGCTGTAGACGGAAGCGAAGCGAGTTGTGACGGTGGGAGTTACGTTTCCGTTACTTACGATGGAAGCTGGTGCCATTGTTTGTTGAGAGAGTGAGAGAGAGAGTGTGTGTGTTGTTGAGAAGTCCCCCCGTACTCTGCGTTGATAC
TARGET=1036,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.0691
PRIMER_LEFT_PENALTY=0.269585
PRIMER_RIGHT_PENALTY=2.799545
PRIMER_LEFT_SEQUENCE=AAACAGTTTCGCGATCTCATCT
PRIMER_RIGHT_SEQUENCE=GTATCAACGCAGAGTACGGGG
PRIMER_LEFT=808,22
PRIMER_RIGHT=1078,21
PRIMER_LEFT_TM=60.270
PRIMER_RIGHT_TM=61.800
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=57.143
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=3.0880
PRIMER_LEFT_1_PENALTY=0.288468
PRIMER_RIGHT_1_PENALTY=2.799545
PRIMER_LEFT_1_SEQUENCE=ACGTTTCCGTTACTTACGATGG
PRIMER_RIGHT_1_SEQUENCE=GTATCAACGCAGAGTACGGGG
PRIMER_LEFT_1=974,22
PRIMER_RIGHT_1=1078,21
PRIMER_LEFT_1_TM=60.288
PRIMER_RIGHT_1_TM=61.800
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=57.143
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=3.1785
PRIMER_LEFT_2_PENALTY=0.378922
PRIMER_RIGHT_2_PENALTY=2.799545
PRIMER_LEFT_2_SEQUENCE=GAAGGAAGACGGAGTTTGTGAT
PRIMER_RIGHT_2_SEQUENCE=GTATCAACGCAGAGTACGGGG
PRIMER_LEFT_2=890,22
PRIMER_RIGHT_2=1078,21
PRIMER_LEFT_2_TM=59.621
PRIMER_RIGHT_2_TM=61.800
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=57.143
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=3.2424
PRIMER_LEFT_3_PENALTY=0.442900
PRIMER_RIGHT_3_PENALTY=2.799545
PRIMER_LEFT_3_SEQUENCE=CTGTTTTGGACTTCGCTGTAGA
PRIMER_RIGHT_3_SEQUENCE=GTATCAACGCAGAGTACGGGG
PRIMER_LEFT_3=920,22
PRIMER_RIGHT_3=1078,21
PRIMER_LEFT_3_TM=59.557
PRIMER_RIGHT_3_TM=61.800
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=57.143
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=12.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=3.2889
PRIMER_LEFT_4_PENALTY=0.489375
PRIMER_RIGHT_4_PENALTY=2.799545
PRIMER_LEFT_4_SEQUENCE=TTCCGTTACTTACGATGGAAGC
PRIMER_RIGHT_4_SEQUENCE=GTATCAACGCAGAGTACGGGG
PRIMER_LEFT_4=978,22
PRIMER_RIGHT_4=1078,21
PRIMER_LEFT_4_TM=60.489
PRIMER_RIGHT_4_TM=61.800
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=12.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_00383 Ps_contig_mira-and-tgicl-ass_franssen_4098
SEQUENCE=ATTTTCGATGATGGCAGGTTTCTTCGTCTTCAGTGCTGTTCATCTTTACAACAAGTAGATCATCGCACGAAGTTAATGTAGTCTGTAACATGGGAGTTGTACTTATATTGAGATTGAAAATTTTGATTAGGCAATGATAAAAATCATAAGACAGGTTGTTTCTAACTTTATTATAGATGTTRCTTCTTTTTATTATTATTATTCTGTCATTTTAATTTTAGTATTTAGTTATGTGAGTATTAATCACTGTAGCAAACCAATTAACTGAAAATTGAAATGACATGAAAAACCACAC
TARGET=190,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00384 Ps_contig_mira-and-tgicl-ass_franssen_4107
SEQUENCE=AACGTAATGCAATACAAAATAACCGAAACTAATTTTTGTAGTTGTACACATGATTCAATAACTAAAATAGCATCAAACTATGGAACCATTCGTAATCAGTGTTTTCGGTTCGAGATATTACCGAATGCAGCACCGTTGATGAAGCGAATCTGGATCTTTCCGTTCCGAGTCTTAACAGAACCCTTACCTGCATAAACAACCAAACAAAAACCAGCAGCGAACCGATCAAAGAAAACCCTCTAACTTGAAATTGTCCAAAACAAAATTGAAGCAAGTGGCATCGTCCTTGTTTGATATCCTATGGCACCACTGAGTTGTTTTCGGTTTGGTGGAAGAAGATCAACGGCGAATGCGTGAGGTCATGGTTTTGACGGTCGTTTGCGTGTCGTTGTCCCAGATGACGGTGACGGACCCGTGTTGTGATGTTGTGAACAGTATTGATGATGGAAGGTTGAGCTATCACCGACAAGATTCTGCCACGGTGAGTGGTCGGATGTGCGGTGAATAGGACGGCGGTTGTGAAAGGATTGGAAAAGAAACGGCGGTCGTTTGAGGTTGTGGTTTTGGGATTCGTCGGAGGGTTAACGGTGAAGAGGGTGAGTTTAAGGGTTTGACGGCAAAGGGTGGTTTAGCGGCGCATGTGGAGATTTTGTGGAGGTGGGTCGGAGGTGCTTTGATGGTGAGAGAGAATTTGGAGAAAGATAAAGAAGATTTTCTGAGATAGAGAGATGAATCCTGGAAAAAGTTTTTGTTTGTTGGTTAATAGAGAGAGTCTGAGTGGTGAGAGAGAGATAGTGAATGAATTTGTTGAGAGTGGTGTGTGTAATGAGTGGTCTCGTCGGAGAGTACCCCGA
TARGET=783,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3507
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.317612
PRIMER_LEFT_SEQUENCE=TAACGGTGAAGAGGGTGAGTTT
PRIMER_RIGHT_SEQUENCE=GTACTCTCCGACGAGACCACTC
PRIMER_LEFT=582,22
PRIMER_RIGHT=848,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.318
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=59.091
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.3507
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.317612
PRIMER_LEFT_1_SEQUENCE=AACGGTGAAGAGGGTGAGTTTA
PRIMER_RIGHT_1_SEQUENCE=GTACTCTCCGACGAGACCACTC
PRIMER_LEFT_1=583,22
PRIMER_RIGHT_1=848,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.318
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=59.091
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.3507
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.317612
PRIMER_LEFT_2_SEQUENCE=TTAACGGTGAAGAGGGTGAGTT
PRIMER_RIGHT_2_SEQUENCE=GTACTCTCCGACGAGACCACTC
PRIMER_LEFT_2=581,22
PRIMER_RIGHT_2=848,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=60.318
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=59.091
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.4750
PRIMER_LEFT_3_PENALTY=0.157415
PRIMER_RIGHT_3_PENALTY=0.317612
PRIMER_LEFT_3_SEQUENCE=CGGTGAAGAGGGTGAGTTTAAG
PRIMER_RIGHT_3_SEQUENCE=GTACTCTCCGACGAGACCACTC
PRIMER_LEFT_3=585,22
PRIMER_RIGHT_3=848,22
PRIMER_LEFT_3_TM=60.157
PRIMER_RIGHT_3_TM=60.318
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=59.091
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=0.6472
PRIMER_LEFT_4_PENALTY=0.329550
PRIMER_RIGHT_4_PENALTY=0.317612
PRIMER_LEFT_4_SEQUENCE=TGATGGTGAGAGAGAATTTGGA
PRIMER_RIGHT_4_SEQUENCE=GTACTCTCCGACGAGACCACTC
PRIMER_LEFT_4=674,22
PRIMER_RIGHT_4=848,22
PRIMER_LEFT_4_TM=59.670
PRIMER_RIGHT_4_TM=60.318
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=59.091
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_00385 Ps_contig_mira-and-tgicl-ass_franssen_4120
SEQUENCE=TTGAAATTCCTATCACATTTCACCCTAATCTAATCTAATCTAATCTGTAATCGACGATTTACATAACATATGGACAACTTTGAGACAACAAATCATAATCTTGTTCATTACAGTATAATTTTGACATGTTATTGCTAGTTACAAATCCTTTTTTGTTATGCTACTTGTAATTTTGTCACTTTCATGAAATTCTATCAGTACTTACAGGGAAATTCAACCCATTCCCTCTTCCAAAG
TARGET=25,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00386 Ps_contig_mira-and-tgicl-ass_franssen_4133
SEQUENCE=CGTTTTCCACCCTTACGTAGAAATTTCCTTTTTCTTGTCTACAGGTGCCAGTCCATCTCTCACAGCAGCCGTTGTTGTGTAACTGGTCTATTGGGGCGGAATTTCGTGCAAGCAACTCTTGATAGACTCAGATTTAGTGATCCGATTGCCCGAGGAATAGCAACAGCTTCTAGTGCACATGGACTTGGAGCAGCAGCATTATCTGCAAAGGAACCTGAGGCTCTCCCATTTTGTGCCATTGCTTATGCTTTGAATGGCATATTTGGATCTTTACTATGTTCAGTTCCTATTGTCAGACAAAGCTTGCTTGCAATTATTGGCTGAAATTGTCATTAATATCACAATCTGTTACCATTTTCCTTCCTTCCTATGTCATAACTTCCATTGTTTTTATTACGGAAGTACTAGTACAAATGTATGTATAGAGAAATATATTGAGAATCATAATTTTGTGAGTTGTATAGGATGCAATAATCTATTCATTTGAGTAATCAAATATCTATGTCATATTCAATTTTTTTTCGTT
TARGET=357,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00387 Ps_contig_mira-and-tgicl-ass_franssen_4135
SEQUENCE=GGGGGGACTCACTCACCACCACCATGGACCCAACTTCCTTCGATCTCCCTCCACCACCACCCACCACCACCACCACCGTCCCCTTCTCCCTGGAACCCCCTTGTTTGGAACCATCATCATCCACTCCTTACCATTGATGTATGGGAGCATGCTTACTATCTTGACTATCAGAACCGGCGCCCTGATTATATATCAGTGTTCATGGATAAGCTTGTGTCTTGGGAAGCAGTCAG
TARGET=61,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0782
PRIMER_LEFT_PENALTY=1.066501
PRIMER_RIGHT_PENALTY=0.011696
PRIMER_LEFT_SEQUENCE=GACCCAACTTCCTTCGATCTC
PRIMER_RIGHT_SEQUENCE=GTAAGCATGCTCCCATACATCA
PRIMER_LEFT=26,21
PRIMER_RIGHT=155,22
PRIMER_LEFT_TM=60.067
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=1.2089
PRIMER_LEFT_1_PENALTY=1.197222
PRIMER_RIGHT_1_PENALTY=0.011696
PRIMER_LEFT_1_SEQUENCE=CAACTTCCTTCGATCTCCCTC
PRIMER_RIGHT_1_SEQUENCE=GTAAGCATGCTCCCATACATCA
PRIMER_LEFT_1=30,21
PRIMER_RIGHT_1=155,22
PRIMER_LEFT_1_TM=60.197
PRIMER_RIGHT_1_TM=59.988
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=1.2531
PRIMER_LEFT_2_PENALTY=1.066501
PRIMER_RIGHT_2_PENALTY=0.186600
PRIMER_LEFT_2_SEQUENCE=GACCCAACTTCCTTCGATCTC
PRIMER_RIGHT_2_SEQUENCE=TGGTAAGGAGTGGATGATGATG
PRIMER_LEFT_2=26,21
PRIMER_RIGHT_2=132,22
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=59.813
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=107
PRIMER_PAIR_PENALTY_3=1.4391
PRIMER_LEFT_3_PENALTY=1.066501
PRIMER_RIGHT_3_PENALTY=0.372557
PRIMER_LEFT_3_SEQUENCE=GACCCAACTTCCTTCGATCTC
PRIMER_RIGHT_3_SEQUENCE=CCCAAGACACAAGCTTATCCAT
PRIMER_LEFT_3=26,21
PRIMER_RIGHT_3=222,22
PRIMER_LEFT_3_TM=60.067
PRIMER_RIGHT_3_TM=60.373
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=1.5429
PRIMER_LEFT_4_PENALTY=1.066501
PRIMER_RIGHT_4_PENALTY=0.476376
PRIMER_LEFT_4_SEQUENCE=GACCCAACTTCCTTCGATCTC
PRIMER_RIGHT_4_SEQUENCE=TAAGCATGCTCCCATACATCAA
PRIMER_LEFT_4=26,21
PRIMER_RIGHT_4=154,22
PRIMER_LEFT_4_TM=60.067
PRIMER_RIGHT_4_TM=60.476
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=129
=
PRIMER_SEQUENCE_ID=SSR_00388 Ps_contig_mira-and-tgicl-ass_franssen_4150
SEQUENCE=CATTTTTTTCCCAATTCTAATTTCATATTACAACCTAAATTTTACCCTTAACAACCCATTAATTAACAAGATCTTACATAAAAACCTCTCAACCACACTACTCCAGACTTTTTTAATTAACATTTCCGCCCCGAGTTTTACCCCATTTTTTAATTCAACCCTTCCGTTTAAAAAAATCACGGTTGAGTTAGAATTTGAAAAAATGGGATAGGGACGTAACGGGTAATAAGAAGATTCTAGATTAAGGAGAGAAAGAGAGAGAGAGGGAGAGAGAGAGAGAGAACAAAAAGGAAGAGAAGATAGAAAGTTAGACGTGGATATGGATATCGACCGTACGGTAAACCCGG
TARGET=254,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.0456
PRIMER_LEFT_PENALTY=0.321492
PRIMER_RIGHT_PENALTY=2.724156
PRIMER_LEFT_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_SEQUENCE=TCGATATCCATATCCACGTCTAAC
PRIMER_LEFT=205,22
PRIMER_RIGHT=329,24
PRIMER_LEFT_TM=60.321
PRIMER_RIGHT_TM=59.276
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=125
PRIMER_PAIR_PENALTY_1=3.2172
PRIMER_LEFT_1_PENALTY=0.321492
PRIMER_RIGHT_1_PENALTY=2.895724
PRIMER_LEFT_1_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_1_SEQUENCE=TCGATATCCATATCCACGTCTA
PRIMER_LEFT_1=205,22
PRIMER_RIGHT_1=329,22
PRIMER_LEFT_1_TM=60.321
PRIMER_RIGHT_1_TM=57.104
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=3.4344
PRIMER_LEFT_2_PENALTY=0.710267
PRIMER_RIGHT_2_PENALTY=2.724156
PRIMER_LEFT_2_SEQUENCE=GGGACGTAACGGGTAATAAGAA
PRIMER_RIGHT_2_SEQUENCE=TCGATATCCATATCCACGTCTAAC
PRIMER_LEFT_2=210,22
PRIMER_RIGHT_2=329,24
PRIMER_LEFT_2_TM=59.290
PRIMER_RIGHT_2_TM=59.276
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=3.4481
PRIMER_LEFT_3_PENALTY=0.321492
PRIMER_RIGHT_3_PENALTY=3.126610
PRIMER_LEFT_3_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_3_SEQUENCE=TCGATATCCATATCCACGTCTAACT
PRIMER_LEFT_3=205,22
PRIMER_RIGHT_3=329,25
PRIMER_LEFT_3_TM=60.321
PRIMER_RIGHT_3_TM=60.127
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=3.6060
PRIMER_LEFT_4_PENALTY=0.710267
PRIMER_RIGHT_4_PENALTY=2.895724
PRIMER_LEFT_4_SEQUENCE=GGGACGTAACGGGTAATAAGAA
PRIMER_RIGHT_4_SEQUENCE=TCGATATCCATATCCACGTCTA
PRIMER_LEFT_4=210,22
PRIMER_RIGHT_4=329,22
PRIMER_LEFT_4_TM=59.290
PRIMER_RIGHT_4_TM=57.104
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_00389 Ps_contig_mira-and-tgicl-ass_franssen_4150
SEQUENCE=CATTTTTTTCCCAATTCTAATTTCATATTACAACCTAAATTTTACCCTTAACAACCCATTAATTAACAAGATCTTACATAAAAACCTCTCAACCACACTACTCCAGACTTTTTTAATTAACATTTCCGCCCCGAGTTTTACCCCATTTTTTAATTCAACCCTTCCGTTTAAAAAAATCACGGTTGAGTTAGAATTTGAAAAAATGGGATAGGGACGTAACGGGTAATAAGAAGATTCTAGATTAAGGAGAGAAAGAGAGAGAGAGGGAGAGAGAGAGAGAGAACAAAAAGGAAGAGAAGATAGAAAGTTAGACGTGGATATGGATATCGACCGTACGGTAAACCCGG
TARGET=267,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.0456
PRIMER_LEFT_PENALTY=0.321492
PRIMER_RIGHT_PENALTY=2.724156
PRIMER_LEFT_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_SEQUENCE=TCGATATCCATATCCACGTCTAAC
PRIMER_LEFT=205,22
PRIMER_RIGHT=329,24
PRIMER_LEFT_TM=60.321
PRIMER_RIGHT_TM=59.276
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=125
PRIMER_PAIR_PENALTY_1=3.2172
PRIMER_LEFT_1_PENALTY=0.321492
PRIMER_RIGHT_1_PENALTY=2.895724
PRIMER_LEFT_1_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_1_SEQUENCE=TCGATATCCATATCCACGTCTA
PRIMER_LEFT_1=205,22
PRIMER_RIGHT_1=329,22
PRIMER_LEFT_1_TM=60.321
PRIMER_RIGHT_1_TM=57.104
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=3.4344
PRIMER_LEFT_2_PENALTY=0.710267
PRIMER_RIGHT_2_PENALTY=2.724156
PRIMER_LEFT_2_SEQUENCE=GGGACGTAACGGGTAATAAGAA
PRIMER_RIGHT_2_SEQUENCE=TCGATATCCATATCCACGTCTAAC
PRIMER_LEFT_2=210,22
PRIMER_RIGHT_2=329,24
PRIMER_LEFT_2_TM=59.290
PRIMER_RIGHT_2_TM=59.276
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=3.4481
PRIMER_LEFT_3_PENALTY=0.321492
PRIMER_RIGHT_3_PENALTY=3.126610
PRIMER_LEFT_3_SEQUENCE=GGATAGGGACGTAACGGGTAAT
PRIMER_RIGHT_3_SEQUENCE=TCGATATCCATATCCACGTCTAACT
PRIMER_LEFT_3=205,22
PRIMER_RIGHT_3=329,25
PRIMER_LEFT_3_TM=60.321
PRIMER_RIGHT_3_TM=60.127
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=3.6060
PRIMER_LEFT_4_PENALTY=0.710267
PRIMER_RIGHT_4_PENALTY=2.895724
PRIMER_LEFT_4_SEQUENCE=GGGACGTAACGGGTAATAAGAA
PRIMER_RIGHT_4_SEQUENCE=TCGATATCCATATCCACGTCTA
PRIMER_LEFT_4=210,22
PRIMER_RIGHT_4=329,22
PRIMER_LEFT_4_TM=59.290
PRIMER_RIGHT_4_TM=57.104
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_00390 Ps_contig_mira-and-tgicl-ass_franssen_4151
SEQUENCE=CTTTTAACCTAACCCTTTAAACCCGTTTTAAAAAACTTAACTACGGTTAGGTAGTTAAGTAATTAAGTAAAAGTAGGAGTAGGGACGGTAACGGTAAGTAAGAAGATNTANATAGAGAGAAGAGAGAGAGRRGAGAGAGAGAGAGACAAARAGGAAGAGAAGATGAAAAGTTGACGTGGATATGGATATGACCTGTAACCCGGAAATGACTCGTTTCAGACTCATGCAATACGAAGAAGACGTCTCAGATCCCGTGTTGCGGAGTCGTCGAAGGCCACCGGCGCAAACTTCCTCTGAAAAGACGGAATCGGAAGTGCGTTCGGCGACGGAGACATCGCGAACGCGGATCCGGCGTAAACTTCGCAACCAGTCTTGAAATCAACGATCCGGATCTGTTTAGGAACCATATTCGGGTCGGGTACGAGTCTCTGTGTTCCAATGACAACCAAATCGTCACCTTCCTTCTTCAAACCCTCGTTGTTCATCTTCGAATCAAGAGATTCGCCTCTCCTGAGAATCATCACCTTCTCCATCATCATCTCACTACCTTTTGCCATCTTCAAATCATCGTAGCTAGGTCTTCTCTCCGTTGTCATTTTCAAATCATCATAACTAGGTCTTTTCTCCGCCACCGCAACACGTTTTCTCTGTTCCGGTCGAATAACCGGTCTCCGGTTAGTGTTAACCCTCCCGGAGTTTGTTGCCCCATAAAAAGTATTACTATTCCCATTACCATAATTGTTATAATTATTATGGCAATTTGTATAAGTTCTTCGTCGGGAAAAACTTGTCGGAGGAACTCTGATTCGTTCATGCAAACAATCATGTGGTAGCAAAATTTCTGTTCCCATGGTGAATGGTAAAAAAATTCAAAAACTGAGAGAGGAAATGAAGAAGAAAAAAAGTATATGAGCGTTTAGATCTGAATAATTAATCAAAACGACAAAACTATTGTCGTTTTGTTAATTTTATGTTTAGAATTTCTATTCTATTGTAATAATATTATATTCTTCTCTGTTTTGTTTCTTCTTCTTCCTCTGAGAAGACAAGGAAGAGAAGAAGGGGGTGTTGATAAAAAAAAATTAGGAAGAAACAAGAAGAAAAGAAATGAAGAAAATATGAATTTTCGSGAACCCCC
TARGET=121,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00391 Ps_contig_mira-and-tgicl-ass_franssen_4151
SEQUENCE=CTTTTAACCTAACCCTTTAAACCCGTTTTAAAAAACTTAACTACGGTTAGGTAGTTAAGTAATTAAGTAAAAGTAGGAGTAGGGACGGTAACGGTAAGTAAGAAGATNTANATAGAGAGAAGAGAGAGAGRRGAGAGAGAGAGAGACAAARAGGAAGAGAAGATGAAAAGTTGACGTGGATATGGATATGACCTGTAACCCGGAAATGACTCGTTTCAGACTCATGCAATACGAAGAAGACGTCTCAGATCCCGTGTTGCGGAGTCGTCGAAGGCCACCGGCGCAAACTTCCTCTGAAAAGACGGAATCGGAAGTGCGTTCGGCGACGGAGACATCGCGAACGCGGATCCGGCGTAAACTTCGCAACCAGTCTTGAAATCAACGATCCGGATCTGTTTAGGAACCATATTCGGGTCGGGTACGAGTCTCTGTGTTCCAATGACAACCAAATCGTCACCTTCCTTCTTCAAACCCTCGTTGTTCATCTTCGAATCAAGAGATTCGCCTCTCCTGAGAATCATCACCTTCTCCATCATCATCTCACTACCTTTTGCCATCTTCAAATCATCGTAGCTAGGTCTTCTCTCCGTTGTCATTTTCAAATCATCATAACTAGGTCTTTTCTCCGCCACCGCAACACGTTTTCTCTGTTCCGGTCGAATAACCGGTCTCCGGTTAGTGTTAACCCTCCCGGAGTTTGTTGCCCCATAAAAAGTATTACTATTCCCATTACCATAATTGTTATAATTATTATGGCAATTTGTATAAGTTCTTCGTCGGGAAAAACTTGTCGGAGGAACTCTGATTCGTTCATGCAAACAATCATGTGGTAGCAAAATTTCTGTTCCCATGGTGAATGGTAAAAAAATTCAAAAACTGAGAGAGGAAATGAAGAAGAAAAAAAGTATATGAGCGTTTAGATCTGAATAATTAATCAAAACGACAAAACTATTGTCGTTTTGTTAATTTTATGTTTAGAATTTCTATTCTATTGTAATAATATTATATTCTTCTCTGTTTTGTTTCTTCTTCTTCCTCTGAGAAGACAAGGAAGAGAAGAAGGGGGTGTTGATAAAAAAAAATTAGGAAGAAACAAGAAGAAAAGAAATGAAGAAAATATGAATTTTCGSGAACCCCC
TARGET=133,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00392 Ps_contig_mira-and-tgicl-ass_franssen_4151
SEQUENCE=CTTTTAACCTAACCCTTTAAACCCGTTTTAAAAAACTTAACTACGGTTAGGTAGTTAAGTAATTAAGTAAAAGTAGGAGTAGGGACGGTAACGGTAAGTAAGAAGATNTANATAGAGAGAAGAGAGAGAGRRGAGAGAGAGAGAGACAAARAGGAAGAGAAGATGAAAAGTTGACGTGGATATGGATATGACCTGTAACCCGGAAATGACTCGTTTCAGACTCATGCAATACGAAGAAGACGTCTCAGATCCCGTGTTGCGGAGTCGTCGAAGGCCACCGGCGCAAACTTCCTCTGAAAAGACGGAATCGGAAGTGCGTTCGGCGACGGAGACATCGCGAACGCGGATCCGGCGTAAACTTCGCAACCAGTCTTGAAATCAACGATCCGGATCTGTTTAGGAACCATATTCGGGTCGGGTACGAGTCTCTGTGTTCCAATGACAACCAAATCGTCACCTTCCTTCTTCAAACCCTCGTTGTTCATCTTCGAATCAAGAGATTCGCCTCTCCTGAGAATCATCACCTTCTCCATCATCATCTCACTACCTTTTGCCATCTTCAAATCATCGTAGCTAGGTCTTCTCTCCGTTGTCATTTTCAAATCATCATAACTAGGTCTTTTCTCCGCCACCGCAACACGTTTTCTCTGTTCCGGTCGAATAACCGGTCTCCGGTTAGTGTTAACCCTCCCGGAGTTTGTTGCCCCATAAAAAGTATTACTATTCCCATTACCATAATTGTTATAATTATTATGGCAATTTGTATAAGTTCTTCGTCGGGAAAAACTTGTCGGAGGAACTCTGATTCGTTCATGCAAACAATCATGTGGTAGCAAAATTTCTGTTCCCATGGTGAATGGTAAAAAAATTCAAAAACTGAGAGAGGAAATGAAGAAGAAAAAAAGTATATGAGCGTTTAGATCTGAATAATTAATCAAAACGACAAAACTATTGTCGTTTTGTTAATTTTATGTTTAGAATTTCTATTCTATTGTAATAATATTATATTCTTCTCTGTTTTGTTTCTTCTTCTTCCTCTGAGAAGACAAGGAAGAGAAGAAGGGGGTGTTGATAAAAAAAAATTAGGAAGAAACAAGAAGAAAAGAAATGAAGAAAATATGAATTTTCGSGAACCCCC
TARGET=1024,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00393 Ps_contig_mira-and-tgicl-ass_franssen_4153
SEQUENCE=TCATATCCATATCCACGTCAACTTTTCATCTTCTCTTCCTTTTTGTCTCTCTCTCTCTCTCCCTCTCTCTCTCTTTCTCTCTCTTGTTAACCAAAATGGTACTTTA
TARGET=46,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00394 Ps_contig_mira-and-tgicl-ass_franssen_4153
SEQUENCE=TCATATCCATATCCACGTCAACTTTTCATCTTCTCTTCCTTTTTGTCTCTCTCTCTCTCTCCCTCTCTCTCTCTTTCTCTCTCTTGTTAACCAAAATGGTACTTTA
TARGET=63,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00395 Ps_contig_mira-and-tgicl-ass_franssen_4171
SEQUENCE=AATTCATTCCTCCATTCCTATGTTGATTTCGTTTGTTCCTTTTGTTTTTTGTTTTTGTCTGYATTTCCTATTAATTGGAATTGGATTTTTTTTTTATATTTTTTTGGTGCTATTTAGTTACCAGATCTGGTTTTTAATTCTGGCAATTAGTATGTCTAAGTCTTGTTGAAGGGTTGTATTTTAATTTTAAATTTTAATGAGTATTGATAAATATATATATATTGAATTTAGGG
TARGET=211,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00396 Ps_contig_mira-and-tgicl-ass_franssen_4179
SEQUENCE=CGGTCCTRMAATTAATAATAATAATTTATAGATAAATAYTATTCAAACATTAGGTTACAATTATTTGGTTGCTTTTTGCAGATGACCACTGTTAAAATGGAGCATGATGCGACTATATACCATACTATATTTGCTTCTCTTTCCCTCTCTCTCCAAGTAGTCCTTACGAAACCCAACATGCATTCATGAGATCTGTTACCTTAACGCAAGCTATTGTTA
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00397 Ps_contig_mira-and-tgicl-ass_franssen_4181
SEQUENCE=CTTACTTTGGAGTTTGTGGTCTTAACCTTCGTAAAGACTACATAATTATCTGACTTGTTAGATAATTGCAAAGAGCATGAGATCTGCTTCCTCAATTCAAAAGGGAACTGTAGCTCCTGAGGGTGTATCTGGAGGAGCTCGCTGGAACTCATGGCGGAGAGATCGGAGAAGAATCGGCGGAGACTAAGATCAGTGTTGATTTTCCGGTGAAGAAAAACGGTTGTGTTAGAGAGAGAGAGAGAGAGAGAGAGAGGGAGAAGAACTAATAACGTCCGCGTACT
TARGET=228,26
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3797
PRIMER_LEFT_PENALTY=0.193133
PRIMER_RIGHT_PENALTY=0.186529
PRIMER_LEFT_SEQUENCE=GAACTGTAGCTCCTGAGGGTGT
PRIMER_RIGHT_SEQUENCE=CGCGGACGTTATTAGTTCTTCT
PRIMER_LEFT=104,22
PRIMER_RIGHT=276,22
PRIMER_LEFT_TM=59.807
PRIMER_RIGHT_TM=59.813
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.4076
PRIMER_LEFT_1_PENALTY=0.221115
PRIMER_RIGHT_1_PENALTY=0.186529
PRIMER_LEFT_1_SEQUENCE=GGCGGAGACTAAGATCAGTGTT
PRIMER_RIGHT_1_SEQUENCE=CGCGGACGTTATTAGTTCTTCT
PRIMER_LEFT_1=175,22
PRIMER_RIGHT_1=276,22
PRIMER_LEFT_1_TM=59.779
PRIMER_RIGHT_1_TM=59.813
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=0.5005
PRIMER_LEFT_2_PENALTY=0.193133
PRIMER_RIGHT_2_PENALTY=0.307346
PRIMER_LEFT_2_SEQUENCE=GAACTGTAGCTCCTGAGGGTGT
PRIMER_RIGHT_2_SEQUENCE=ACGCGGACGTTATTAGTTCTTC
PRIMER_LEFT_2=104,22
PRIMER_RIGHT_2=277,22
PRIMER_LEFT_2_TM=59.807
PRIMER_RIGHT_2_TM=59.693
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.5285
PRIMER_LEFT_3_PENALTY=0.221115
PRIMER_RIGHT_3_PENALTY=0.307346
PRIMER_LEFT_3_SEQUENCE=GGCGGAGACTAAGATCAGTGTT
PRIMER_RIGHT_3_SEQUENCE=ACGCGGACGTTATTAGTTCTTC
PRIMER_LEFT_3=175,22
PRIMER_RIGHT_3=277,22
PRIMER_LEFT_3_TM=59.779
PRIMER_RIGHT_3_TM=59.693
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=0.6412
PRIMER_LEFT_4_PENALTY=0.454655
PRIMER_RIGHT_4_PENALTY=0.186529
PRIMER_LEFT_4_SEQUENCE=TCCTCAATTCAAAAGGGAACTG
PRIMER_RIGHT_4_SEQUENCE=CGCGGACGTTATTAGTTCTTCT
PRIMER_LEFT_4=88,22
PRIMER_RIGHT_4=276,22
PRIMER_LEFT_4_TM=60.455
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_00398 Ps_contig_mira-and-tgicl-ass_franssen_4194
SEQUENCE=ACAAGGCGTTGGTAATCTTCAAATCATGTTTGGATCTATTGGTTTGAGGCCTGCTGTTGACACACGTTGGAGTTCACCGCACCGTTATTCTTTTCTTCATTGCGTTTCCGGTTAGTAATCCCTGAGCATTAGATTTTATTTTATTTTTTATATATATTGTTGAGGGTCGTGTTTGTTCTCAAATGAAAAAGCAGAGGGATGTTGCAAATCAAAGGGAGTGGATTTTAGCGTTTTTCTTTGTTATTGTTCTGGAACATCATAAGTCTTAATTAATTTTATTATTATTATTTCATCCCTATTAATCTATTTGGGATTTAGAAGTCATTTGGRCTGWATWTTTTTTACAACCTCAAGCAAACTCTAAATTTAMAWAWAMMMWGNTTGTTATGTACCACCCATTTTATTACTTTTATTGTCATTTTTTGTCTTTTCTTATTCAAAATTAAGGATCGAACCGTAAGGGTTGTATTAAGAGGACCTATTGGGTACC
TARGET=133,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00399 Ps_contig_mira-and-tgicl-ass_franssen_4194
SEQUENCE=ACAAGGCGTTGGTAATCTTCAAATCATGTTTGGATCTATTGGTTTGAGGCCTGCTGTTGACACACGTTGGAGTTCACCGCACCGTTATTCTTTTCTTCATTGCGTTTCCGGTTAGTAATCCCTGAGCATTAGATTTTATTTTATTTTTTATATATATTGTTGAGGGTCGTGTTTGTTCTCAAATGAAAAAGCAGAGGGATGTTGCAAATCAAAGGGAGTGGATTTTAGCGTTTTTCTTTGTTATTGTTCTGGAACATCATAAGTCTTAATTAATTTTATTATTATTATTTCATCCCTATTAATCTATTTGGGATTTAGAAGTCATTTGGRCTGWATWTTTTTTACAACCTCAAGCAAACTCTAAATTTAMAWAWAMMMWGNTTGTTATGTACCACCCATTTTATTACTTTTATTGTCATTTTTTGTCTTTTCTTATTCAAAATTAAGGATCGAACCGTAAGGGTTGTATTAAGAGGACCTATTGGGTACC
TARGET=276,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00400 Ps_contig_mira-and-tgicl-ass_franssen_4196
SEQUENCE=TATATAAAAAATAAAATAAAATCTAATGCTCAGGGATTACCAACAGCAAACACAATGATGAAAATAACGGTGCGGTGAACTCCGACGTGCGTCAACAGCAGGCATCAAACCAAATAGATCCAAACATGATTTGAAGATWACCAACGCCTTGTCAACACGGTGTTGTCGACATCATGAATCCATGGTTA
TARGET=8,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00401 Ps_contig_mira-and-tgicl-ass_franssen_4217
SEQUENCE=GCCTTAAAGCAATATAAATAAATAAAAGTATTAGATCATAGAATCATACYYWTTACATCCCTCACTTTTATTATTTATTTCCACCTTCTCTCATAGAAAGTGAGGAATATTTAATTTGCATGACTAWTTTAACTTAGGAAAGTCAAGTCACACTACTATTGCATCATATACAAATGACAACATAGAATAACCAAAATAAAATACGTTCAGAGTGCTCTTATTAGAGTCCAAT
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00402 Ps_contig_mira-and-tgicl-ass_franssen_4248
SEQUENCE=AAGCTAAGTCTAGATTGGGATTGTCTTATGCTAAAAGGGAAGATGGCAAGGAGTCTATCTTTGGTGATGAGTCAAGAGTAACTATTCAAGAAGTTGAAGTGTTTTGTAGCCCTAAAATTGCAAGCTTATATTAGAATTTCATGAAAAATACTATACTATACTAATTCTCCTACTTTGAAGCATTTGTTTTTCTTTTATCAGAAAGAGAGATTGTAAAACTTTAAAACTAAGTACTGCTTATTATTATACTATTACTTAAAGTTAATTAATAAGAAAAAAACCT
TARGET=148,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00403 Ps_contig_mira-and-tgicl-ass_franssen_4262
SEQUENCE=GCAGAGTACGTCGGGATCATCATCAGAAGAAAAAGATACTATGTGGTGAAACCGAGGGCCAAACTCCAACAACAACAAAAATGGCGCTTCAAACGCAATTCGCAACTTATTTCACCACCAAAATCTATCATCATTCGTTCTCTCCCATCCTCACAGTTAGATCTGAAAAGCATCTGAACCGTCCGNTTCAACTTACCCCAAAAAAACGTCCGGGGGTTGGGTTTTGGTCCTCTAATGTGAACACTAAAAGGAAGCAAACAACTAAAGGAAG
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2423
PRIMER_LEFT_PENALTY=1.021310
PRIMER_RIGHT_PENALTY=0.220992
PRIMER_LEFT_SEQUENCE=CGGGATCATCATCAGAAGAAA
PRIMER_RIGHT_SEQUENCE=TCACATTAGAGGACCAAAACCC
PRIMER_LEFT=11,21
PRIMER_RIGHT=239,22
PRIMER_LEFT_TM=60.021
PRIMER_RIGHT_TM=60.221
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=1.2653
PRIMER_LEFT_1_PENALTY=1.021310
PRIMER_RIGHT_1_PENALTY=0.243965
PRIMER_LEFT_1_SEQUENCE=CGGGATCATCATCAGAAGAAA
PRIMER_RIGHT_1_SEQUENCE=GGTGGTGAAATAAGTTGCGAAT
PRIMER_LEFT_1=11,21
PRIMER_RIGHT_1=118,22
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=60.244
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=108
PRIMER_PAIR_PENALTY_2=1.2703
PRIMER_LEFT_2_PENALTY=1.132848
PRIMER_RIGHT_2_PENALTY=0.137489
PRIMER_LEFT_2_SEQUENCE=TACTATGTGGTGAAACCGAGGG
PRIMER_RIGHT_2_SEQUENCE=ACGGTTCAGATGCTTTTCAGAT
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=181,22
PRIMER_LEFT_2_TM=61.133
PRIMER_RIGHT_2_TM=60.137
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=1.2802
PRIMER_LEFT_3_PENALTY=1.021310
PRIMER_RIGHT_3_PENALTY=0.258908
PRIMER_LEFT_3_SEQUENCE=CGGGATCATCATCAGAAGAAA
PRIMER_RIGHT_3_SEQUENCE=CATTAGAGGACCAAAACCCAAC
PRIMER_LEFT_3=11,21
PRIMER_RIGHT_3=236,22
PRIMER_LEFT_3_TM=60.021
PRIMER_RIGHT_3_TM=59.741
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=1.2802
PRIMER_LEFT_4_PENALTY=1.021310
PRIMER_RIGHT_4_PENALTY=0.258908
PRIMER_LEFT_4_SEQUENCE=CGGGATCATCATCAGAAGAAA
PRIMER_RIGHT_4_SEQUENCE=ACATTAGAGGACCAAAACCCAA
PRIMER_LEFT_4=11,21
PRIMER_RIGHT_4=237,22
PRIMER_LEFT_4_TM=60.021
PRIMER_RIGHT_4_TM=59.741
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_00404 Ps_contig_mira-and-tgicl-ass_franssen_4263
SEQUENCE=TCGGGGGKTGGTGAAACCGAGGGCCAAACTCCAACAACAACAAAAAATGGCGCTTCAAACGCAATTCGCAACTTATTTCACCACCAAAATACTATCATCATTCGTTCTCTCCCATCCTCACAGTTAGATCTGAAAAGCAATCTGAACCGTCCGATTCAATTCCCCAAAAAAACTACCGGGGTGGGTTTTGGTCCGTCTAATGTGAACACTAAAGGAAGCAAACACTAAAGGAAGCAGAAGGGACAAGAGAAGAGCTTCGATAA
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00405 Ps_contig_mira-and-tgicl-ass_franssen_4264
SEQUENCE=GTCGGGGATCATTATCATCATCAGAAGAAAAAAGATACTATGTGGTGAAACCGAGGGCCAAACTCCAACAAACAAACAAAAAATGGCGCTTCAAACGCAATTCGCAACTTATTTCACCACCAAAATCTATCATCATTCGTTCTCTCCCATCCTCACAGTTAGATCTGAAAAGCAATCTGAACCGTCCGATTCAATTCCAAAAATCCGGGTGGTTTGGTCCGTCTAATGTGAACACTAAAAGGAAGCAAAAA
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2350
PRIMER_LEFT_PENALTY=1.132848
PRIMER_RIGHT_PENALTY=0.102129
PRIMER_LEFT_SEQUENCE=TACTATGTGGTGAAACCGAGGG
PRIMER_RIGHT_SEQUENCE=GTGTTCACATTAGACGGACCAA
PRIMER_LEFT=35,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=61.133
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=200
PRIMER_PAIR_PENALTY_1=1.3264
PRIMER_LEFT_1_PENALTY=1.224301
PRIMER_RIGHT_1_PENALTY=0.102129
PRIMER_LEFT_1_SEQUENCE=GATACTATGTGGTGAAACCGAGG
PRIMER_RIGHT_1_SEQUENCE=GTGTTCACATTAGACGGACCAA
PRIMER_LEFT_1=33,23
PRIMER_RIGHT_1=234,22
PRIMER_LEFT_1_TM=59.776
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=1.5283
PRIMER_LEFT_2_PENALTY=1.132848
PRIMER_RIGHT_2_PENALTY=0.395499
PRIMER_LEFT_2_SEQUENCE=TACTATGTGGTGAAACCGAGGG
PRIMER_RIGHT_2_SEQUENCE=AACTGTGAGGATGGGAGAGAAC
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=159,22
PRIMER_LEFT_2_TM=61.133
PRIMER_RIGHT_2_TM=59.605
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=1.5317
PRIMER_LEFT_3_PENALTY=1.132848
PRIMER_RIGHT_3_PENALTY=0.398877
PRIMER_LEFT_3_SEQUENCE=TACTATGTGGTGAAACCGAGGG
PRIMER_RIGHT_3_SEQUENCE=TGTTCACATTAGACGGACCAAA
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=233,22
PRIMER_LEFT_3_TM=61.133
PRIMER_RIGHT_3_TM=60.399
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=199
PRIMER_PAIR_PENALTY_4=1.6049
PRIMER_LEFT_4_PENALTY=1.132848
PRIMER_RIGHT_4_PENALTY=0.472052
PRIMER_LEFT_4_SEQUENCE=TACTATGTGGTGAAACCGAGGG
PRIMER_RIGHT_4_SEQUENCE=TGGGAGAGAACGAATGATGATA
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=148,22
PRIMER_LEFT_4_TM=61.133
PRIMER_RIGHT_4_TM=59.528
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=114
=
PRIMER_SEQUENCE_ID=SSR_00406 Ps_contig_mira-and-tgicl-ass_franssen_4266
SEQUENCE=AAGTTACACAGCTAATTTTAAGAAGGTAAAGGGTATTAAATGCAGCTCAGTACATAATATATATATACAAAAAGAAGTTTCATAGCTAAGAAAAGAAGTGA
TARGET=57,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00407 Ps_contig_mira-and-tgicl-ass_franssen_4267
SEQUENCE=TCGGGTACSACTTCATTCATTCATTCATTCTCCGATCTTCACCGCTCCGGTTCTTTGATTCCGGTTCACGTAAAAAACCGAAAAAGAATAACCGAACCGTAAAATCTCGGTGCTCTAATGGAAGCTCACGGCGGAGGACTCCGCCGCGTCCTGGTACTAGCCTTCTGCGTCGCCGGAATCTGGTCGGCTTATATCTACCAAGGCATTCTTCAGGAAACACTGTCAACGAAACGATTCGGTCCAGACGGTGAGAGATTTGAACACCTAGCGGTTTTGAACTTGGCACAGAACGTAGTGTGTTTAATCTGGTCATTCATAATGATACAGATGTGGTCTGGTGGAATTTCTGGTGGTGCTCCTTGGTGGTCTTATTGGAGTGCTGGTATTACTAACACCATTGGTCCTGCTATGGGAATTGAAGCTTTGAAGTATATTAGTTACCCTGCTCAGGTGCTGGCAAAGTCCTCAAAAATGATTCCAGTTATGCTGATGGGTTCACTCGTATACGGTATAAGATACACCATTCCAGAATACCTTTGCACCTTCCTTGTTGCTGGAGGGGTTTCAA
TARGET=12,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00408 Ps_contig_mira-and-tgicl-ass_franssen_4268
SEQUENCE=GGGGATCACACCATTGAGTGGAGAGTGGTATATCGAACAACATTAGAATTAAATATTAAGAAAAAAAAGAAAGAGTGTAAGGTTGTGAGGTACAGAGGAGATAGAAAAAGGAAGTGAGAACAGAGAGAGTGAAGWMTGAAAATAGATAGATAGATCTGAAGAAAAAAATGTCGACGGGTTAGTAGATCTGTATTCGAAGGGGTATTTAGGTTAAGAGTTGGGTTGTTTTTTGGG
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00409 Ps_contig_mira-and-tgicl-ass_franssen_4282
SEQUENCE=CTTAAGTATTCAAGTGTTTTCGGTTTGAATTTCGAAACAACAACAACTGGGTGGAGTGGGTGGGTTGGAAGGGAAAAATTAGGGGAGGGAAAAAAGGGTAGCATAGATAGTGTTTGTGATATGGCTTCTGCTAGCCTTGGAAGTGGAAATGGAAATGGTGGTGTTGGTAGTTCTAGGTCTGCTTTAAGAGGTTCTTCAAGTTCTGTTGATTGGCTTGGGAGAGAGATGCTTGAGATGAGGCTCAGAGATAAAGTTGAGCATGACCATGATGACGATGATGATGGCAGGGAGAGTGAGCCTGATATCATTGATGGTGTGGGTGCTGAAACAGGACATGTAATACGAACCAGCATTGGTGGACGAAATGGTCAATCTAAGCAGAATGTTAGTTATATTGCGGAACACGTAGTTGGGACGGGCTCTTTTGGCACTGTTTTTCAGGCAAAATGTAGAGAAACAGGAGAGATTGTTGCCATCA
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00410 Ps_contig_mira-and-tgicl-ass_franssen_4302
SEQUENCE=TACCGTTTTTGTTTTTATTATTAATGTAATACAACAATTAACGCACAAATAAAATAATACAAGATTGTATTTTGTATTAGAGAGGAAAGATCAGTATCAAGAAAAGAAGAGACGACATTATATATACTTTTGAATCATTTCTCTGACTTCTTCAATAACTAGCCATGCCTTATGACCTCCAATCACTTCTGCTTTCATTTCTCCATCTTTCCAAAGCTGAATTGTTGGCATTTTAGAAATGTTGCCACGCTTGACTAGAGACTGAGGTACTTTATTAACATCCACATAGTAAATTTGTTCTGCAAAGTAAGAAAATAAATAATTAGTAGAAAGAGTAATTGAATCAAATTAATTAATTAAGTAAAATGTAATGAAAGGAGTTAGTTTTACTTGGTGTACTAAATTCAGCTGTCTTTAA
TARGET=347,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.8973
PRIMER_LEFT_PENALTY=0.139083
PRIMER_RIGHT_PENALTY=4.758221
PRIMER_LEFT_SEQUENCE=ATGACCTCCAATCACTTCTGCT
PRIMER_RIGHT_SEQUENCE=GACAGCTGAATTTAGTACACCAAG
PRIMER_LEFT=171,22
PRIMER_RIGHT=412,24
PRIMER_LEFT_TM=60.139
PRIMER_RIGHT_TM=57.242
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=4.9509
PRIMER_LEFT_1_PENALTY=0.139083
PRIMER_RIGHT_1_PENALTY=4.811858
PRIMER_LEFT_1_SEQUENCE=ATGACCTCCAATCACTTCTGCT
PRIMER_RIGHT_1_SEQUENCE=AGACAGCTGAATTTAGTACACCAAG
PRIMER_LEFT_1=171,22
PRIMER_RIGHT_1=413,25
PRIMER_LEFT_1_TM=60.139
PRIMER_RIGHT_1_TM=58.188
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=4.9734
PRIMER_LEFT_2_PENALTY=0.215225
PRIMER_RIGHT_2_PENALTY=4.758221
PRIMER_LEFT_2_SEQUENCE=ATGCCTTATGACCTCCAATCAC
PRIMER_RIGHT_2_SEQUENCE=GACAGCTGAATTTAGTACACCAAG
PRIMER_LEFT_2=164,22
PRIMER_RIGHT_2=412,24
PRIMER_LEFT_2_TM=60.215
PRIMER_RIGHT_2_TM=57.242
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=5.0013
PRIMER_LEFT_3_PENALTY=0.243031
PRIMER_RIGHT_3_PENALTY=4.758221
PRIMER_LEFT_3_SEQUENCE=GACCTCCAATCACTTCTGCTTT
PRIMER_RIGHT_3_SEQUENCE=GACAGCTGAATTTAGTACACCAAG
PRIMER_LEFT_3=173,22
PRIMER_RIGHT_3=412,24
PRIMER_LEFT_3_TM=59.757
PRIMER_RIGHT_3_TM=57.242
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=5.0013
PRIMER_LEFT_4_PENALTY=0.243031
PRIMER_RIGHT_4_PENALTY=4.758221
PRIMER_LEFT_4_SEQUENCE=ACCTCCAATCACTTCTGCTTTC
PRIMER_RIGHT_4_SEQUENCE=GACAGCTGAATTTAGTACACCAAG
PRIMER_LEFT_4=174,22
PRIMER_RIGHT_4=412,24
PRIMER_LEFT_4_TM=59.757
PRIMER_RIGHT_4_TM=57.242
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_00411 Ps_contig_mira-and-tgicl-ass_franssen_4321
SEQUENCE=GAAGAGAAGATGTTCCATTACWCGGCASKGGCCTACACCACTCCACGTTACCATCTTTGTCCGSAAAACCAACAATTCCATACCCATCTTCGTCCTCACAAAATTAAATATCTCCATCTACATACACTACACCTTTTCAATACTCAACTCTTCTCTCTCTCTCTCTTTTCTACCATGGTTGCAAGCTTGGGAAACCTAGCGTTATTACTCGACATTTCTTCGCCGCGAACCTTATCTCTCGATAGAAAGTCTCGTCTCGTTGGCGTAGACGCAATTCTCACCTTACCGCTCAAGCGAGACTCTTCTTCTTCTTCTTCTACTTCCTCCTCTTGCGGATACGATGGGGAGGTACGTGGGCGCGTGGTGGTGGCGCGTGGGAAGTCAAACTCGGAGCAGAACGGGGTTGATTTTGACGCGGAGTGGAGTGACGAGGAAGAAGGAGAGTTTGGGGATGATTTTGAGAAGATAAGGATGAGAGTGAGGGAGCTTGAGGAGAGGAGAGTGAGGGAGCTTGAGGAGAGGAGAGAGCTTGAGAAGAAAACGGAAGAGTATTTGCAGAGTGAAAAAATCGATGATGGAGATGAAGAGATCACCGAGGAAGAGAAGAGGATGAGAGTCAGGAAAGTGCTCGAAAAGGTGGCTAAGGAGCAGGCAGAGAAGAGGGCCATGGCTCAGTTGATGTTCGATTTGGGTCAGAAAGCTTACGGGAGAGGCAAGTATGGACGGGCCATTAAATTTCTTGAAGGTGCGCTCACTATCATAGCAAGACCTACATTATTCGGTGGTGAGATCCAAATATGGTTGGCCATGGCTTATGAGGCCAATAATCGCCATAACGATTGCATTGATCTATACAAGCAATTAGAAAAGTCACACCCTATTGTTAGCATCCGGCGCCAAGCTGCAGAGCTTCGTTACATTTTGGAAGCACCAAAGCTAAAGATAACCCAGGAGGAGATGTTATGCTGTAACATGGAGTGATAAAAACAAGGGTAGAAAGAGTGGAACAGTTAACACTCCATTTCCATCATCAAAAGATAACCTTTTGGATTCTCTTGTGTGGAGCCCTCCTACTGGATTAGGGAAAAATCGAGCATTCTGGGTTGGTTTAACATTATGGTTGGGATTAGTAGGTGCTGCTCTCTTCATTCAAAGATGAAGTTCCAGTTCTTCAATTTCTGATGATTCTAATTTTGTGTTATAATTTTTCATTTTAGTTTTTAGTGTATATAGTCCATTGTAAAATTATTGTATGTAACCAATAGGAAATATGTGTTATTTTATTATATTTTCCTGTTAGGGTGGTTGTTCTATTGAAAGTACAAGATTGTAAGTTCAAATTTAATTGTGTCATTGTATATTGAAAAAATAATTGTTGAATTACAAATATTTATGGTAATTTCTGTTAAACTT
TARGET=152,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00412 Ps_contig_mira-and-tgicl-ass_franssen_4321
SEQUENCE=GAAGAGAAGATGTTCCATTACWCGGCASKGGCCTACACCACTCCACGTTACCATCTTTGTCCGSAAAACCAACAATTCCATACCCATCTTCGTCCTCACAAAATTAAATATCTCCATCTACATACACTACACCTTTTCAATACTCAACTCTTCTCTCTCTCTCTCTTTTCTACCATGGTTGCAAGCTTGGGAAACCTAGCGTTATTACTCGACATTTCTTCGCCGCGAACCTTATCTCTCGATAGAAAGTCTCGTCTCGTTGGCGTAGACGCAATTCTCACCTTACCGCTCAAGCGAGACTCTTCTTCTTCTTCTTCTACTTCCTCCTCTTGCGGATACGATGGGGAGGTACGTGGGCGCGTGGTGGTGGCGCGTGGGAAGTCAAACTCGGAGCAGAACGGGGTTGATTTTGACGCGGAGTGGAGTGACGAGGAAGAAGGAGAGTTTGGGGATGATTTTGAGAAGATAAGGATGAGAGTGAGGGAGCTTGAGGAGAGGAGAGTGAGGGAGCTTGAGGAGAGGAGAGAGCTTGAGAAGAAAACGGAAGAGTATTTGCAGAGTGAAAAAATCGATGATGGAGATGAAGAGATCACCGAGGAAGAGAAGAGGATGAGAGTCAGGAAAGTGCTCGAAAAGGTGGCTAAGGAGCAGGCAGAGAAGAGGGCCATGGCTCAGTTGATGTTCGATTTGGGTCAGAAAGCTTACGGGAGAGGCAAGTATGGACGGGCCATTAAATTTCTTGAAGGTGCGCTCACTATCATAGCAAGACCTACATTATTCGGTGGTGAGATCCAAATATGGTTGGCCATGGCTTATGAGGCCAATAATCGCCATAACGATTGCATTGATCTATACAAGCAATTAGAAAAGTCACACCCTATTGTTAGCATCCGGCGCCAAGCTGCAGAGCTTCGTTACATTTTGGAAGCACCAAAGCTAAAGATAACCCAGGAGGAGATGTTATGCTGTAACATGGAGTGATAAAAACAAGGGTAGAAAGAGTGGAACAGTTAACACTCCATTTCCATCATCAAAAGATAACCTTTTGGATTCTCTTGTGTGGAGCCCTCCTACTGGATTAGGGAAAAATCGAGCATTCTGGGTTGGTTTAACATTATGGTTGGGATTAGTAGGTGCTGCTCTCTTCATTCAAAGATGAAGTTCCAGTTCTTCAATTTCTGATGATTCTAATTTTGTGTTATAATTTTTCATTTTAGTTTTTAGTGTATATAGTCCATTGTAAAATTATTGTATGTAACCAATAGGAAATATGTGTTATTTTATTATATTTTCCTGTTAGGGTGGTTGTTCTATTGAAAGTACAAGATTGTAAGTTCAAATTTAATTGTGTCATTGTATATTGAAAAAATAATTGTTGAATTACAAATATTTATGGTAATTTCTGTTAAACTT
TARGET=301,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00413 Ps_contig_mira-and-tgicl-ass_franssen_4342
SEQUENCE=TATTTGAACCTTGAAGGACAAAGCAATGAAACTTGCATCTTGAAACCAATGAAGATTCATTTTTCTCTTGTAGATTTTCATAAATCAGAAGTATGACCATCCTAAGTCCTATTGTATATATTGAAAATCACGAAATTATTTTTCATGCTCATAAATAAACTATTCTTGAAAAGTATGATCACAATGAAATCAATTTATTTATTTACATGTACATAATATCTTTTTGGTCAATAGATTATTGGATTGAAATAWAATTGTCACAGGTTACTCTTGCTATTGATATTGTGTTTGAATGTATAATTAGGAACTTAGGATCGAATAAGTACAATCACTATTTCGATAGTATACAAGTAATAGTGTGATCACACTGCTCACTACTTTTATTATTCCTA
TARGET=193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00414 Ps_contig_mira-and-tgicl-ass_franssen_4357
SEQUENCE=CTTGGAGCAACATGTGTTTACCCATTACAGGTKGGGAGAACCTTGCGGGTGAATTTCTCGGACAAACCGAAGCCGAAAGAACCGTTGTACCCTGAATCCGAATACAAGCTTTTCGTTGGGAACTTGTCTTGGACAGTAACTTCTGAGAGTCTGGCAGAAGTCTTTCAACAACATGGAACTGTGGTTGGAGCTAGGGTGTTGTATGATGGCGAAACGGGAAAGTCTCGTGGCTATGGCTTTGTTTGCTATGCCAATAAATCAGAAATGGAAGCTGCCCTTACAGTCATGAATGATGTGGAACTAGATGGACGGACATTGCGACGKAAGTTTGGCGCANNAAACGGAAAAACGTACTACTAAAGTTAACGGGTACGTAAGTAAACGAAAACCAACGAAAAAGGAAAAACGTACCGTCCGACGAAGAGTTGGTTACGAAACTAAGTTGGTTGGTCGAACCGAAAAGAAAGTAGGAAGGAAGGAAGG
TARGET=469,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00415 Ps_contig_mira-and-tgicl-ass_franssen_4358
SEQUENCE=ATGGGTGGGGGTGGGCGGGTGGGTGGGTGGTGCTGCAAGAAAGAAACCACGGGTGGGTGGGAAGTAGAAAGAAAAATAGAATATTATTATAATCCACCAAGGTATGGTATGGTAGTTTTATGCTTATCCTGGACCAGTTTACCCTCCACAGTATTTTCAGTGATGACGAGAACCCCAATGCTTGTTCTGTAATGTAAAAATTATTCAAGAATGGAAATGGTAAGGGTAATATTGGAATTATGAAATTTAGACTCTATGAGTGAAATGTGTTTGTGATAGCCATATCCTGGGTAAGATGTGCCCTATGTACTTTTCCCTGCAGTAGAAAAGTGTAATCTACAGATAACTGTAGAGTTTTGAAATGCTTGTACTTTTTTTATTTAACCAAAATTAATTGTTATATATGAAAAATATATAAATAATTAAAGTGTTTTATCTWWAACT
TARGET=17,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00416 Ps_contig_mira-and-tgicl-ass_franssen_4359
SEQUENCE=TGGTATCAACGACAGAGAACGAGACACTATATTTCATACATAAAAAAACACCGCAAATAAAAGTAGCCGCAGAACCATCCTCGCTTATCTCTCTATCTCTCTCTAACCTTTCAGTTCAGTTACACTAAATCAATGGGAGAGGAAGAAAAGAAACCAGAGGAAACTAAAGTTGAGGAGAAAAAACCAGAAGAACCGAAGAAAGAAGAAGAGAAGAAAGAAGATGAAAAAGCCACAGAGGAGAAAAAGCCGGAGGAATCTAAGGAACCGGTTGCCCCGCCGGAAATTGTGCTTAAAGTTTTCATGCATTGTGAAGGCTGTGCTCGTAAAGTTCGTCGTTCACTCAAAGGATTTCCAGGTGTTGAAGATGTTGTAACTGATTGCAAATCTCACAAGGTTGTTGTGAAAGGAGAGAAAGCGGATCCATTGAAGGTTCTAGAAAGAGTACAAAGGAAGAGTCATAGGCAAGTTGAGCTTCTTTCTCCGATCCCGATACCACCGTCGGAAGAAGATAAAAAATCGGAAGAGAAAGAGAAACCTAAACAAGAGGAAGAGAAGAAAGAAGAGCCTCAAGTTATCACAGTGATTCTGAAAGTTCACATGCATTGTGAAGCTTGTTCACAGGAAATCAAAAGACGCATTGAGAGAATCAAAGGAGTGGAATCAGCAGAACCGGATCTGAAGAACTCACTAGTGACAGTGAAAGGAGTGTTTGAAAGTGAGAAATTAGTTGAATATGTTTACAAGAGAACCGGAAAACAAGCGGTGATAGTGAAACAAGAGGCGGAGAAGAAAGAAGAAGCGAAAGAAACAAAGGAAGAAGAGAAGAAAACTGAGGAGAAGGACAAGAATGAGAAAGGAGGTGGTGAAGGAGAGGAAAAGAAAGAAGCTGGAGAAGGAGATGAAAAATCAGATGGTGGGGGTGGCGGTGGTGGTGGTGCTGCAGAAGAAACCACGGTGGTGGAAGTGAAGAAAAATGAATATTATTATAATCCACCAAGGTATGGTATGGAGTTTTATGCTTATCCTGGACCAGTTTACCCTCCACAGATTTTCAGTGATGAGAACCCCAATGCTTGTTCTGTAATGTAAAAAATTATTCAAGAATGGAAATGGTAAGGGTAATATTGGAATTATGAAATTTAGACTCTATGAGTGAAATGTGTTTGTGATAGCCATATCCTGGGTAAGATGTGCCCTATGTACTTTTCCCTGCAGTAGAAAAGTGTAATCTACAGATAACTGTAGAGTTTTGAAATGCTTGTACTTTTTTTATTTAACCAAAATTAATTGTTATATATGAAAAAATATATAAATAATTAAAGTGTTTTATACTTY
TARGET=925,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00417 Ps_contig_mira-and-tgicl-ass_franssen_4371
SEQUENCE=GAAAGTGAAGAGAGGAGGTGAACATTACGAAGTAACAACAACAACACTTATTTCGTCAACTACTTCGCCATTTGCATTACATCTCTTCTCTATAACATAATAAACTCTTCACACTTTACCCATTCTCTGCAAACTCTCGTTTCTTCCATGGATTCCTCCGCCGCCATTCGATCTTCTCACTGCTTTATGTCCCATATGCAATCCTCATTTCAGAAACCAGTTGTAATTCCCATAAAAATGTTGGATGGAACTTTCAAAGTAGGACTTTCATCCAGAGCTTGGCAGTTAGTGTTAAGCACAATGATTCTCACAACAAATGGAAGAAAACACTAGTGTCTTGCTCAAAACCAACAGAAGCTGTTGACACTACCAAGTCCGAGGCTTCTTTGGACAACATTACAAAAGAATCATTGGAGAAAAATCCTTTGCAAACAGCTACATTTCCTAATGGATTTGAGGCTTTACTATTAGATATATGCGATGAGACTCAAATTGCTGAAGTGAAATTAAAGGTTGGAGAGTTTGAGATGCATATGAAACGAAGCATGGGAGTAGCAGATGCTCCAATTTCCGACATTTCACAAGCTACACCATCGCCTGTTCCAAACTAAACTCATAGTTGAATCAACAACTTCRACCCCACCACCTAAACCATCTCCAGGAAAAACCAATCCATTTGTAAATGAGTTCCATGATAAGTCACCAAAGTTGGCTGCATTGGAGGCATCTAAGAMCMAAAATTATCTCTTGGTAGAATCTCCTATGGTATGSYWTATWWCGAAGAGGTAAAACTGTGAAAGGGAA
TARGET=34,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00418 Ps_contig_mira-and-tgicl-ass_franssen_4376
SEQUENCE=GATAGAAAAAAGAAAAGAAAAAAGGAAAAGAGAAAAGCTGTACAGCTTTTCCTCCCCCACCTTCCTTCCTTCTTCGCCACCCACACAAAACGACAACCGCCGCTGCTCCCCTTTCCACTTCCATCATTGCCAACACCTCCACGTAACCGTTCTCCGTCCGCAACCCATCTTCATCATTCATCTCCACCGCAAAAACCCACACGTCGGCGGACTCAAACAACCACACAACACACGTCGCCGGCAAAAATCCTTCTCCAACCGCCGTGACCTAAAGCCGACGAACACCACCATCGCAACGCCCTTCTTCCGCTTCACCCTCACAAACCCTCCTTCTCCGAATCTCCTTCGCCAACAAGGAAAATCCGACCGCCGTACCACCACACACGCGACTCGCATCTCCGATCTACTTTTCCATTTCGATTTCAACTTCACCCTTCCACCATCACAGTTTCACCATTACATACCCTTGAAACCACCTTCAATCTATTCAATCTA
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.2099
PRIMER_LEFT_PENALTY=3.103576
PRIMER_RIGHT_PENALTY=0.106310
PRIMER_LEFT_SEQUENCE=GTACAGCTTTTCCTCCCCCAC
PRIMER_RIGHT_SEQUENCE=TGGAGATGAATGATGAAGATGG
PRIMER_LEFT=39,21
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=62.104
PRIMER_RIGHT_TM=59.894
PRIMER_LEFT_GC_PERCENT=57.143
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=147
PRIMER_PAIR_PENALTY_1=3.3169
PRIMER_LEFT_1_PENALTY=3.210543
PRIMER_RIGHT_1_PENALTY=0.106310
PRIMER_LEFT_1_SEQUENCE=CTGTACAGCTTTTCCTCCCC
PRIMER_RIGHT_1_SEQUENCE=TGGAGATGAATGATGAAGATGG
PRIMER_LEFT_1=37,20
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=58.789
PRIMER_RIGHT_1_TM=59.894
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=3.3193
PRIMER_LEFT_2_PENALTY=3.213030
PRIMER_RIGHT_2_PENALTY=0.106310
PRIMER_LEFT_2_SEQUENCE=CTGTACAGCTTTTCCTCCCCC
PRIMER_RIGHT_2_SEQUENCE=TGGAGATGAATGATGAAGATGG
PRIMER_LEFT_2=37,21
PRIMER_RIGHT_2=185,22
PRIMER_LEFT_2_TM=62.213
PRIMER_RIGHT_2_TM=59.894
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=3.4768
PRIMER_LEFT_3_PENALTY=3.370497
PRIMER_RIGHT_3_PENALTY=0.106310
PRIMER_LEFT_3_SEQUENCE=TACAGCTTTTCCTCCCCCAC
PRIMER_RIGHT_3_SEQUENCE=TGGAGATGAATGATGAAGATGG
PRIMER_LEFT_3=40,20
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=61.370
PRIMER_RIGHT_3_TM=59.894
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=3.4768
PRIMER_LEFT_4_PENALTY=3.370497
PRIMER_RIGHT_4_PENALTY=0.106310
PRIMER_LEFT_4_SEQUENCE=GTACAGCTTTTCCTCCCCCA
PRIMER_RIGHT_4_SEQUENCE=TGGAGATGAATGATGAAGATGG
PRIMER_LEFT_4=39,20
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=61.370
PRIMER_RIGHT_4_TM=59.894
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_00419 Ps_contig_mira-and-tgicl-ass_franssen_4402
SEQUENCE=GACTACAACAACAAGAATCACCTTCTGTACCCARWMRRKKYAATTTGACTTCTTTTTCTCTAACCCTTCTTCTTCTTCTTCTTCACCGATTCTTCTGAACCTGAAAATACTACAACATTGCCACATGAATAATTAGGGTTTACACACAACAAAGTTGAAACGATTTCCGAAATGAGCGGTTCCAGATTCATAAAGTGTGTCACAGTAGGCGATGGAGCCGTTGGCAAGACTTTGTTTGCTTATCT
TARGET=66,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00420 Ps_contig_mira-and-tgicl-ass_franssen_4414
SEQUENCE=GAATATTCAATTTCTATCCACTATTCATCTTCTTCTTCTTCTGTTTCTCATCTAGGGTTTTCTATTCCATTTCCATGGCCACCGCAATTCGAACTCCCTTCTTCTCCTCCCACCACCACCA
TARGET=28,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00421 Ps_contig_mira-and-tgicl-ass_franssen_4414
SEQUENCE=GAATATTCAATTTCTATCCACTATTCATCTTCTTCTTCTTCTGTTTCTCATCTAGGGTTTTCTATTCCATTTCCATGGCCACCGCAATTCGAACTCCCTTCTTCTCCTCCCACCACCACCA
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_00422 Ps_contig_mira-and-tgicl-ass_franssen_4425
SEQUENCE=GGCCACCGGAGCTAACAATTCTCCTGTCAAAACAAATTGACGAGAAAGAGAAGCCGTTAGAAACGGGAGTTTTCCGTAGACGGAAGTTATGCGGCGGAGGAGCGGTGGAGGAAGCGACTCGGAAGATAGTAGCCATGGTAATGGTTTGTTACTCTAGAAGAAGAAGAAGAAGAAGAATTGAGAAATTGAGGAAGAGGAGAATAATTAGGGTTAAGGAATTGCGTGAGGGAGGAGAAAGAGAAAGAGAAAGGGAAATGGTATTTCCTTTCTTTCTTTCTTGTTGGTTTTGATTCACTCCCTCACTCACAAGAGAACTCAGACACTCTCCCCGAGTACTCTGCGTTG
TARGET=156,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0283
PRIMER_LEFT_PENALTY=0.002623
PRIMER_RIGHT_PENALTY=0.025669
PRIMER_LEFT_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_RIGHT_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT=56,22
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=59.997
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.1482
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.025669
PRIMER_LEFT_1_SEQUENCE=GGAAGCGACTCGGAAGATAGTA
PRIMER_RIGHT_1_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=4.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.1542
PRIMER_LEFT_2_PENALTY=0.002623
PRIMER_RIGHT_2_PENALTY=0.151533
PRIMER_LEFT_2_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_RIGHT_2_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=59.997
PRIMER_RIGHT_2_TM=59.848
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.1680
PRIMER_LEFT_3_PENALTY=0.142283
PRIMER_RIGHT_3_PENALTY=0.025669
PRIMER_LEFT_3_SEQUENCE=CGAGAAAGAGAAGCCGTTAGAA
PRIMER_RIGHT_3_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=312,22
PRIMER_LEFT_3_TM=60.142
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.2577
PRIMER_LEFT_4_PENALTY=0.232060
PRIMER_RIGHT_4_PENALTY=0.025669
PRIMER_LEFT_4_SEQUENCE=CGACTCGGAAGATAGTAGCCAT
PRIMER_RIGHT_4_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_4=114,22
PRIMER_RIGHT_4=312,22
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=60.026
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_00423 Ps_contig_mira-and-tgicl-ass_franssen_4425
SEQUENCE=GGCCACCGGAGCTAACAATTCTCCTGTCAAAACAAATTGACGAGAAAGAGAAGCCGTTAGAAACGGGAGTTTTCCGTAGACGGAAGTTATGCGGCGGAGGAGCGGTGGAGGAAGCGACTCGGAAGATAGTAGCCATGGTAATGGTTTGTTACTCTAGAAGAAGAAGAAGAAGAAGAATTGAGAAATTGAGGAAGAGGAGAATAATTAGGGTTAAGGAATTGCGTGAGGGAGGAGAAAGAGAAAGAGAAAGGGAAATGGTATTTCCTTTCTTTCTTTCTTGTTGGTTTTGATTCACTCCCTCACTCACAAGAGAACTCAGACACTCTCCCCGAGTACTCTGCGTTG
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0283
PRIMER_LEFT_PENALTY=0.002623
PRIMER_RIGHT_PENALTY=0.025669
PRIMER_LEFT_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_RIGHT_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT=56,22
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=59.997
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.1482
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.025669
PRIMER_LEFT_1_SEQUENCE=GGAAGCGACTCGGAAGATAGTA
PRIMER_RIGHT_1_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=4.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.1542
PRIMER_LEFT_2_PENALTY=0.002623
PRIMER_RIGHT_2_PENALTY=0.151533
PRIMER_LEFT_2_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_RIGHT_2_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=59.997
PRIMER_RIGHT_2_TM=59.848
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.1680
PRIMER_LEFT_3_PENALTY=0.142283
PRIMER_RIGHT_3_PENALTY=0.025669
PRIMER_LEFT_3_SEQUENCE=CGAGAAAGAGAAGCCGTTAGAA
PRIMER_RIGHT_3_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=312,22
PRIMER_LEFT_3_TM=60.142
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.2577
PRIMER_LEFT_4_PENALTY=0.232060
PRIMER_RIGHT_4_PENALTY=0.025669
PRIMER_LEFT_4_SEQUENCE=CGACTCGGAAGATAGTAGCCAT
PRIMER_RIGHT_4_SEQUENCE=TCTCTTGTGAGTGAGGGAGTGA
PRIMER_LEFT_4=114,22
PRIMER_RIGHT_4=312,22
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=60.026
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_00424 Ps_contig_mira-and-tgicl-ass_franssen_4459
SEQUENCE=GAAACTGTGAGACTTTCCAGCTGCCATTTCAACGCCAAAACAATCATCTACAGAGCTATTTTCAATGTCAAACGCAGTCCATCTGGGGTGTTGTTGAAGGAGGATAGTGTGATGAGAAGGTTGTTGAAGAAGGAAAGGAGAAGAAGAAGAAGAATGATGATGAGATTGGGAGTCGAGGAGGTGCTGAAGCTGACCGATTAGCTGCTTCAGCATGGCCAGGCCTTCCATCGCCCCCCCCGCGTACTTCCCCCGCAAT
TARGET=139,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1659
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=1.029845
PRIMER_LEFT_SEQUENCE=GGGTGTTGTTGAAGGAGGATAG
PRIMER_RIGHT_SEQUENCE=CTAATCGGTCAGCTTCAGCAC
PRIMER_LEFT=85,22
PRIMER_RIGHT=200,21
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=1.2971
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=1.161090
PRIMER_LEFT_1_SEQUENCE=GGGTGTTGTTGAAGGAGGATAG
PRIMER_RIGHT_1_SEQUENCE=CAGCTAATCGGTCAGCTTCAG
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=203,21
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.161
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=119
PRIMER_PAIR_PENALTY_2=1.4713
PRIMER_LEFT_2_PENALTY=0.481423
PRIMER_RIGHT_2_PENALTY=0.989871
PRIMER_LEFT_2_SEQUENCE=CAACGCCAAAACAATCATCTAC
PRIMER_RIGHT_2_SEQUENCE=CTCGACTCCCAATCTCATCATC
PRIMER_LEFT_2=29,22
PRIMER_RIGHT_2=176,22
PRIMER_LEFT_2_TM=59.519
PRIMER_RIGHT_2_TM=60.990
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=1.5076
PRIMER_LEFT_3_PENALTY=0.481423
PRIMER_RIGHT_3_PENALTY=1.026160
PRIMER_LEFT_3_SEQUENCE=CAACGCCAAAACAATCATCTAC
PRIMER_RIGHT_3_SEQUENCE=TCGACTCCCAATCTCATCATC
PRIMER_LEFT_3=29,22
PRIMER_RIGHT_3=175,21
PRIMER_LEFT_3_TM=59.519
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=1.5113
PRIMER_LEFT_4_PENALTY=0.481423
PRIMER_RIGHT_4_PENALTY=1.029845
PRIMER_LEFT_4_SEQUENCE=CAACGCCAAAACAATCATCTAC
PRIMER_RIGHT_4_SEQUENCE=CTAATCGGTCAGCTTCAGCAC
PRIMER_LEFT_4=29,22
PRIMER_RIGHT_4=200,21
PRIMER_LEFT_4_TM=59.519
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_00425 Ps_contig_mira-and-tgicl-ass_franssen_4464
SEQUENCE=TTCATGCAACCACTGCTTGACATTGTTGAAACTTTCCATATCAGTTACATCATAAACAATAATGATTCCATGTGCTCCTCTATAATAACTGCTAGTTATAGTCCTGAATCGCTCTTGTCCAGCAGAAGTCACAATTTGCAGTTTGACGGTTTTGCCTTCAAGTTCAACTGTTCTGATTTTCTGGCATAAAAAGTGTGAATGAGTATATAATTGATGGATAGAATAGAATAGAAAAGAATAGAAAATATAGTTACAAAATCGACGCCAATGGTGCTAATGTAGCTGTCAACATAAGAGTCATCCTAGAAAAAAAGTTAGGGTTAGGGATGGAAAAGGTCAAGTAAGTAAAAAGGATGTGAGTAGAAAAGGAACGGACGGCGAATCTGAGAAGCAGGA
TARGET=218,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2892
PRIMER_LEFT_PENALTY=0.219884
PRIMER_RIGHT_PENALTY=0.069319
PRIMER_LEFT_SEQUENCE=TGTCCAGCAGAAGTCACAATTT
PRIMER_RIGHT_SEQUENCE=ACCTTTTCCATCCCTAACCCTA
PRIMER_LEFT=115,22
PRIMER_RIGHT=336,22
PRIMER_LEFT_TM=59.780
PRIMER_RIGHT_TM=60.069
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.3530
PRIMER_LEFT_1_PENALTY=0.283676
PRIMER_RIGHT_1_PENALTY=0.069319
PRIMER_LEFT_1_SEQUENCE=CAACTGTTCTGATTTTCTGGCA
PRIMER_RIGHT_1_SEQUENCE=ACCTTTTCCATCCCTAACCCTA
PRIMER_LEFT_1=164,22
PRIMER_RIGHT_1=336,22
PRIMER_LEFT_1_TM=60.284
PRIMER_RIGHT_1_TM=60.069
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.3844
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.164560
PRIMER_LEFT_2_SEQUENCE=TGTCCAGCAGAAGTCACAATTT
PRIMER_RIGHT_2_SEQUENCE=CGTCCGTTCCTTTTCTACTCAC
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=376,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=60.165
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=262
PRIMER_PAIR_PENALTY_3=0.4482
PRIMER_LEFT_3_PENALTY=0.283676
PRIMER_RIGHT_3_PENALTY=0.164560
PRIMER_LEFT_3_SEQUENCE=CAACTGTTCTGATTTTCTGGCA
PRIMER_RIGHT_3_SEQUENCE=CGTCCGTTCCTTTTCTACTCAC
PRIMER_LEFT_3=164,22
PRIMER_RIGHT_3=376,22
PRIMER_LEFT_3_TM=60.284
PRIMER_RIGHT_3_TM=60.165
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.6680
PRIMER_LEFT_4_PENALTY=0.598673
PRIMER_RIGHT_4_PENALTY=0.069319
PRIMER_LEFT_4_SEQUENCE=TTGCCTTCAAGTTCAACTGTTC
PRIMER_RIGHT_4_SEQUENCE=ACCTTTTCCATCCCTAACCCTA
PRIMER_LEFT_4=151,22
PRIMER_RIGHT_4=336,22
PRIMER_LEFT_4_TM=59.401
PRIMER_RIGHT_4_TM=60.069
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_00426 Ps_contig_mira-and-tgicl-ass_franssen_4469
SEQUENCE=AGATGATATAACCTTCAAATTTGCTTATCATGTTTCAATGATGATCACAAAGTAAAATCAAAGACAACACAAAATATCRAATATTCCACATCATTAATAACCTTTTAGAATCTACATTCTTCTCGCCCGGGTTAATAAACACAATTCTATATATTGGATTTGTTGCAATGATAACTGAATAAAAAGGAAAAGAAAAGAAAAATCAGTAGTTG
TARGET=187,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00427 Ps_contig_mira-and-tgicl-ass_franssen_4492
SEQUENCE=AAGTAAATAACAAAACGACCCAAACAAAAAATAGTAATTTAAATTTTAAACTAAAAGTTTAACAGCAGACAACTAGCACTTAATAATTTCGTCCTATTACAATGAGATTATTCTTATTTTGTATACCAAATGTCAAGATTATTGCAATGTAGTACTCATACACATACATAATAAGAACATAATGTATTATAAATGTTTTTGGTCCATTATCCCAAAGCCAATGTGTAACTCATTCCTTCCATCTCTGCTCATGAGCAAATGAAGCTTCAAGAGCACATTGCCTTCTCCACAACTTCCTGTAATTCTCCATTCTTATATGCCTCAACAGTGATATCACATCCACCGAAAAACTCTCCATCAATGTATAGCTGAGGAAAAGTAGGCCAACTGCTATACTCCTTAAGTCCCTGGCGCACCAAATCATTATCCAATATGTTTACAGTTTCAAAAGAAGCATTCAAAGACTTCAAAATTTGCACCACTGTATTTGAGAATCCACACTGTGGAAAATCCCTAGTACCCTTCATAAACAAAACTATTTTGTTTGAAGTAACAACTTTATCCAACGTGGTCTTCAATTCAGGAGTCAACGCAGAGCAGCGAGTTGTGGTGGAAGAGCGTTTGAGATGTAGTTTGGGTTGGGAATGGAAAACGGTGTTTTGAGAGAGAAGAGGAGATGAACGAGGTAGTAGAGACAGGGACGAGGTTGTTGTTGTTGTTGTTGTTCCAGAAACGTGGGAGTGGAAGCATGATGTTGTCGGGTTGAATTGTAGCTGTGAAGAAATTGGGTTAACGCACCATGTGAAAGACATGTTCTGAGTTCTAACTTCCACTCACAATTCTCTTCCAACAAATATCACTTGTTTGATTTCTCCGATTTTTTTTTCTCAACTCCCCCGAC
TARGET=706,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3081
PRIMER_LEFT_PENALTY=0.038090
PRIMER_RIGHT_PENALTY=0.269968
PRIMER_LEFT_SEQUENCE=ACGTGGTCTTCAATTCAGGAGT
PRIMER_RIGHT_SEQUENCE=TACAATTCAACCCGACAACATC
PRIMER_LEFT=565,22
PRIMER_RIGHT=771,22
PRIMER_LEFT_TM=60.038
PRIMER_RIGHT_TM=59.730
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.3269
PRIMER_LEFT_1_PENALTY=0.038090
PRIMER_RIGHT_1_PENALTY=0.288804
PRIMER_LEFT_1_SEQUENCE=ACGTGGTCTTCAATTCAGGAGT
PRIMER_RIGHT_1_SEQUENCE=TTGGAAGAGAATTGTGAGTGGA
PRIMER_LEFT_1=565,22
PRIMER_RIGHT_1=849,22
PRIMER_LEFT_1_TM=60.038
PRIMER_RIGHT_1_TM=59.711
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.4139
PRIMER_LEFT_2_PENALTY=0.143968
PRIMER_RIGHT_2_PENALTY=0.269968
PRIMER_LEFT_2_SEQUENCE=GGAAAACGGTGTTTTGAGAGAG
PRIMER_RIGHT_2_SEQUENCE=TACAATTCAACCCGACAACATC
PRIMER_LEFT_2=646,22
PRIMER_RIGHT_2=771,22
PRIMER_LEFT_2_TM=60.144
PRIMER_RIGHT_2_TM=59.730
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.4328
PRIMER_LEFT_3_PENALTY=0.143968
PRIMER_RIGHT_3_PENALTY=0.288804
PRIMER_LEFT_3_SEQUENCE=GGAAAACGGTGTTTTGAGAGAG
PRIMER_RIGHT_3_SEQUENCE=TGGAAGAGAATTGTGAGTGGAA
PRIMER_LEFT_3=646,22
PRIMER_RIGHT_3=848,22
PRIMER_LEFT_3_TM=60.144
PRIMER_RIGHT_3_TM=59.711
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.4328
PRIMER_LEFT_4_PENALTY=0.143968
PRIMER_RIGHT_4_PENALTY=0.288804
PRIMER_LEFT_4_SEQUENCE=GGAAAACGGTGTTTTGAGAGAG
PRIMER_RIGHT_4_SEQUENCE=TTGGAAGAGAATTGTGAGTGGA
PRIMER_LEFT_4=646,22
PRIMER_RIGHT_4=849,22
PRIMER_LEFT_4_TM=60.144
PRIMER_RIGHT_4_TM=59.711
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=204
=
PRIMER_SEQUENCE_ID=SSR_00428 Ps_contig_mira-and-tgicl-ass_franssen_4493
SEQUENCE=GACAATCATTAATATACATGGCTAGAATGAAAATAACAAGATTCGCCACAACAAACAAAGGAACAAGCCATGATGTCCAACTAGTTTTCTTGGATCATAAACACGTGAAGGAGGAGGAGGAATAGAAGAAGAATTAGTTCTTTGTTTCGCCCCTTCTCTCTCAAGATCCTCCTAACCAGTACTTATTTTTCTCTCTCTACGAACCAGAGAGAACTAAATTTACGTAAATTTAGTAGGAACGTAAAGGGTTTGTAGGTAGTGGAAGAAGGAGTAGGGTAAGGGGTTGGGGTTT
TARGET=109,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5344
PRIMER_LEFT_PENALTY=0.306698
PRIMER_RIGHT_PENALTY=0.227726
PRIMER_LEFT_SEQUENCE=CACAACAAACAAAGGAACAAGC
PRIMER_RIGHT_SEQUENCE=CAACCCCTTACCCTACTCCTTC
PRIMER_LEFT=46,22
PRIMER_RIGHT=285,22
PRIMER_LEFT_TM=59.693
PRIMER_RIGHT_TM=60.228
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.6521
PRIMER_LEFT_1_PENALTY=0.424392
PRIMER_RIGHT_1_PENALTY=0.227726
PRIMER_LEFT_1_SEQUENCE=ACAACAAACAAAGGAACAAGCC
PRIMER_RIGHT_1_SEQUENCE=CAACCCCTTACCCTACTCCTTC
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=285,22
PRIMER_LEFT_1_TM=60.424
PRIMER_RIGHT_1_TM=60.228
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.6585
PRIMER_LEFT_2_PENALTY=0.306698
PRIMER_RIGHT_2_PENALTY=0.351777
PRIMER_LEFT_2_SEQUENCE=CACAACAAACAAAGGAACAAGC
PRIMER_RIGHT_2_SEQUENCE=AGGGGCGAAACAAAGAACTAAT
PRIMER_LEFT_2=46,22
PRIMER_RIGHT_2=153,22
PRIMER_LEFT_2_TM=59.693
PRIMER_RIGHT_2_TM=60.352
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.6757
PRIMER_LEFT_3_PENALTY=0.447971
PRIMER_RIGHT_3_PENALTY=0.227726
PRIMER_LEFT_3_SEQUENCE=CCACAACAAACAAAGGAACAAG
PRIMER_RIGHT_3_SEQUENCE=CAACCCCTTACCCTACTCCTTC
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=285,22
PRIMER_LEFT_3_TM=59.552
PRIMER_RIGHT_3_TM=60.228
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=0.7290
PRIMER_LEFT_4_PENALTY=0.501224
PRIMER_RIGHT_4_PENALTY=0.227726
PRIMER_LEFT_4_SEQUENCE=AACAAGCCATGATGTCCAACTA
PRIMER_RIGHT_4_SEQUENCE=CAACCCCTTACCCTACTCCTTC
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=285,22
PRIMER_LEFT_4_TM=59.499
PRIMER_RIGHT_4_TM=60.228
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_00429 Ps_contig_mira-and-tgicl-ass_franssen_4534
SEQUENCE=CTAATRGTAAGAAAATTGTGTCCAAGATTAAGTTCGAATAGAAAATTTCTTGAGTACAGATGTGATGCATAATCTAAATTAAAAGTTACATTCAAGCTTAAGAAAAGACTTCATAAATTTATTACTTTCTCTATGGTACAAGAAAGAAAGTTTATTGGATCAAACGAAACTACACATGAAGAAAAGTAGACAAATGGAAATAAATATATATATGATCAANAATTGGATACACATATAGAACATTAAA
TARGET=204,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00430 Ps_contig_mira-and-tgicl-ass_franssen_4542
SEQUENCE=AGAAAAACCATAAGAGCTACATCTGCATCACATAGAGTTGAAAGCTCCTGAGCTTTCTTGAAAAGCCCTTTCCTCCTCTTAGAGAAAGTCACTTGCCTTGAACTGATGTTGTCAATTTTCTTGATCTGTATCTTCTTTCTTGTCATACTATTATTATTCCTCTCTCTCTCAAAATTTAGGTTACTTTATGAACTATGAAGAGAGAGAGAAAGCGGAAGTTACAAGAACAAACCGAACCAGTTTMTCTTATTGAGATTCCCCGA
TARGET=160,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00431 Ps_contig_mira-and-tgicl-ass_franssen_4542
SEQUENCE=AGAAAAACCATAAGAGCTACATCTGCATCACATAGAGTTGAAAGCTCCTGAGCTTTCTTGAAAAGCCCTTTCCTCCTCTTAGAGAAAGTCACTTGCCTTGAACTGATGTTGTCAATTTTCTTGATCTGTATCTTCTTTCTTGTCATACTATTATTATTCCTCTCTCTCTCAAAATTTAGGTTACTTTATGAACTATGAAGAGAGAGAGAAAGCGGAAGTTACAAGAACAAACCGAACCAGTTTMTCTTATTGAGATTCCCCGA
TARGET=199,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00432 Ps_contig_mira-and-tgicl-ass_franssen_4560
SEQUENCE=AATAYTAKTAGTTCTAAGAAACCTTACCATTCAACCTCAAAATTTACATTCCCGAAATATTAATGTACAGTTGTACTTTGTTTGTGAGTACATAAAACTACACATGTTCCATATAAAAAAACACAAGGATTTAACTAAAATTCTAAACCAATCACAGAACATGCAAACCAAGTTTTGTTTCTTAAACGTTCTGATCCGTTGATGTTGTTGTTTCTGTTGTAGTTGTTGCTGCTGTTGTTGTTCTTTCGACACTCTCTGTTCTTGGAACACGATTTTCATCACCACTTCCTTGTTTCACACTTGAATCACCATCATCATCATCGTTATCGTTATTGTTATCCGCATCATCGTTCATGTTTTCCTTTAATTTCTGGTTCTGGTCACAGGTACATGTACTAGTGTTATCGCCGAAAGACGAGGATCTACTAGTACCTACGCTTAATGAAGACATGCTTGGTGTTGCTAAGAATGTTGGTTTGGCTTGTCCCGCCATAATCACCAAGATTTTCTCCTCGTAAGGCTTCTGAGGTTTTTGATCTGTGTCGTTTGTTTCTTGCCCAGCTTCAAGGTCTCGTTCGGATTCGCCGTTGCCTTCGAGGTAACCAGAGAGTTTCCAATAAGAACATGCAAGGATTAAGAGAGCGAAAGCTATGAGGCCTAACATGGCTGASCTAAAACCCACCGAAGGAGGGTAAGGGACTGGTAGAATGCCAGTGGTAGG
TARGET=310,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00433 Ps_contig_mira-and-tgicl-ass_franssen_4563
SEQUENCE=GCCAAGCTTTAGTAATACGCTGAGGACCGCGTACACTGACTCTTGTTATTGTGGCGTTTCTTCTTGTTGCATTGTCGTCTGTTGATTCGGCTCTCAGCTACCTTTTGAATTTAGCTCTTCGGAAAAGCACATAACAGTAGCAGTATTGCTCATGTTACCATTACTATCTATTCAAGTAGCTCTTATCGAAGCCGTGGATTCAGGTGAGGAAATTGGTTGCATTGCATGGAATCTTGGTAACATAATTTCATGCATAGAAACTGTATATTGAAATACATATTTGAGGATTATAAGTTTGAAGTGTAAAAAATCGGTTGTATTAAACTGAAAAATCAATTAAGTTCCGCATCGATTAGTGTGTGTGTGTGTGAAAAAATAGAACTTGAGAGTTATTATTCATATGAATAGTACTCCCTCCGTTTCTTTTTAAGTGTCGTTTTAGCATAAAGCTCTCCAACCAAAG
TARGET=356,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3183
PRIMER_LEFT_PENALTY=0.061818
PRIMER_RIGHT_PENALTY=0.256503
PRIMER_LEFT_SEQUENCE=AGTAGCTCTTATCGAAGCCGTG
PRIMER_RIGHT_SEQUENCE=CTTTGGTTGGAGAGCTTTATGC
PRIMER_LEFT=174,22
PRIMER_RIGHT=462,22
PRIMER_LEFT_TM=60.062
PRIMER_RIGHT_TM=60.257
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.4209
PRIMER_LEFT_1_PENALTY=0.061818
PRIMER_RIGHT_1_PENALTY=0.359050
PRIMER_LEFT_1_SEQUENCE=AGTAGCTCTTATCGAAGCCGTG
PRIMER_RIGHT_1_SEQUENCE=CACTTAAAAAGAAACGGAGGGA
PRIMER_LEFT_1=174,22
PRIMER_RIGHT_1=432,22
PRIMER_LEFT_1_TM=60.062
PRIMER_RIGHT_1_TM=59.641
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.4775
PRIMER_LEFT_2_PENALTY=0.220992
PRIMER_RIGHT_2_PENALTY=0.256503
PRIMER_LEFT_2_SEQUENCE=ATTCAGGTGAGGAAATTGGTTG
PRIMER_RIGHT_2_SEQUENCE=CTTTGGTTGGAGAGCTTTATGC
PRIMER_LEFT_2=197,22
PRIMER_RIGHT_2=462,22
PRIMER_LEFT_2_TM=60.221
PRIMER_RIGHT_2_TM=60.257
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.5800
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.359050
PRIMER_LEFT_3_SEQUENCE=ATTCAGGTGAGGAAATTGGTTG
PRIMER_RIGHT_3_SEQUENCE=CACTTAAAAAGAAACGGAGGGA
PRIMER_LEFT_3=197,22
PRIMER_RIGHT_3=432,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.641
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.7758
PRIMER_LEFT_4_PENALTY=0.519311
PRIMER_RIGHT_4_PENALTY=0.256503
PRIMER_LEFT_4_SEQUENCE=CAAGTAGCTCTTATCGAAGCCG
PRIMER_RIGHT_4_SEQUENCE=CTTTGGTTGGAGAGCTTTATGC
PRIMER_LEFT_4=172,22
PRIMER_RIGHT_4=462,22
PRIMER_LEFT_4_TM=60.519
PRIMER_RIGHT_4_TM=60.257
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_00434 Ps_contig_mira-and-tgicl-ass_franssen_4574
SEQUENCE=GGAACTAATAACTCTAAATTTTATTAAGTCATAAAGTAGAAGTATCTAACTCTTCCAAACATGGATTAAATTGGATCAAATAAGAATACCAAACACACTACCTTAAATTAAACTAACAAAACAAATCATGTGAATATGTGAATCACCTAACAACAAACAACTAACAATAAAAAATACGAAGGGGGTAGGAGTTCTACACCTATTTTTTCCAACAGGCAATAGGTGAGCAACAAACAAACAGACAAATAGAAGTAATAGTAACTTAAG
TARGET=229,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00435 Ps_contig_mira-and-tgicl-ass_franssen_4585
SEQUENCE=GATTGYGATTGCAGATAAGAAAGAGTTTCTTCTCTCTTCACCGCCTCAAACCACTCTTCCTATTTTCTCTCTCTCTATTGTCTCTGATTTACAACTAATAACAGTGTAGTTTAGTATCATCATCATTCATCCGGACAGATAAAAACAGAGTCCTCTGTTTCACAGAGTGTTGTGTTGTGTTCTGTTTCCTGCTCTCTCTCAGGTCACTCACAGGCTCTCACCTTTGTTTGGATTCACTGATATTTGAAAAATAGCAGCAGCAGCACCTGATGCAGATGCAGCCTATGATGGCAGCTTACTATCCCAACAACGTCACCACTGATCATATTCAACAGTACCTGGACGAGAACAAGTCCCTGATTCTCAAGATCGTCGAAAGCCAGAACTCTGGCAAACTCACCGAATGTGCCGAGAACCAATCAAGGCTTCAGAGAAATCTCATGTACCTAGCTGCAATAGCTGATTCTCAACCCCAACCACCTACCATGTCTGGTCAGTATCCTTCAAGTGGAATGATGCAGCAGGGAGGACACTACATGCAGGCTCAGCAAGCTCAACAGATGACACAGCAGCAACTGATGGCTGCGCGTTCTTCTCTACTGTATGCTCAACAACTTCAACAGCAGCTTGGCATGAATTCCAGTGGAACTCAAGGTCTTCACAT
TARGET=66,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00436 Ps_contig_mira-and-tgicl-ass_franssen_4585
SEQUENCE=GATTGYGATTGCAGATAAGAAAGAGTTTCTTCTCTCTTCACCGCCTCAAACCACTCTTCCTATTTTCTCTCTCTCTATTGTCTCTGATTTACAACTAATAACAGTGTAGTTTAGTATCATCATCATTCATCCGGACAGATAAAAACAGAGTCCTCTGTTTCACAGAGTGTTGTGTTGTGTTCTGTTTCCTGCTCTCTCTCAGGTCACTCACAGGCTCTCACCTTTGTTTGGATTCACTGATATTTGAAAAATAGCAGCAGCAGCACCTGATGCAGATGCAGCCTATGATGGCAGCTTACTATCCCAACAACGTCACCACTGATCATATTCAACAGTACCTGGACGAGAACAAGTCCCTGATTCTCAAGATCGTCGAAAGCCAGAACTCTGGCAAACTCACCGAATGTGCCGAGAACCAATCAAGGCTTCAGAGAAATCTCATGTACCTAGCTGCAATAGCTGATTCTCAACCCCAACCACCTACCATGTCTGGTCAGTATCCTTCAAGTGGAATGATGCAGCAGGGAGGACACTACATGCAGGCTCAGCAAGCTCAACAGATGACACAGCAGCAACTGATGGCTGCGCGTTCTTCTCTACTGTATGCTCAACAACTTCAACAGCAGCTTGGCATGAATTCCAGTGGAACTCAAGGTCTTCACAT
TARGET=167,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00437 Ps_contig_mira-and-tgicl-ass_franssen_4585
SEQUENCE=GATTGYGATTGCAGATAAGAAAGAGTTTCTTCTCTCTTCACCGCCTCAAACCACTCTTCCTATTTTCTCTCTCTCTATTGTCTCTGATTTACAACTAATAACAGTGTAGTTTAGTATCATCATCATTCATCCGGACAGATAAAAACAGAGTCCTCTGTTTCACAGAGTGTTGTGTTGTGTTCTGTTTCCTGCTCTCTCTCAGGTCACTCACAGGCTCTCACCTTTGTTTGGATTCACTGATATTTGAAAAATAGCAGCAGCAGCACCTGATGCAGATGCAGCCTATGATGGCAGCTTACTATCCCAACAACGTCACCACTGATCATATTCAACAGTACCTGGACGAGAACAAGTCCCTGATTCTCAAGATCGTCGAAAGCCAGAACTCTGGCAAACTCACCGAATGTGCCGAGAACCAATCAAGGCTTCAGAGAAATCTCATGTACCTAGCTGCAATAGCTGATTCTCAACCCCAACCACCTACCATGTCTGGTCAGTATCCTTCAAGTGGAATGATGCAGCAGGGAGGACACTACATGCAGGCTCAGCAAGCTCAACAGATGACACAGCAGCAACTGATGGCTGCGCGTTCTTCTCTACTGTATGCTCAACAACTTCAACAGCAGCTTGGCATGAATTCCAGTGGAACTCAAGGTCTTCACAT
TARGET=253,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00438 Ps_contig_mira-and-tgicl-ass_franssen_4604
SEQUENCE=TTACAACATACAACATCTTAAAAGGTTAATACTAGATATATTAAACAAAAGGTTCATAATCTCCTGCTCATAACTCAGTTAACTAGTGAAGATATGTAGCTTTGGTTACTGATAGCCATTTCCAAAGTAGTTTCCATGATTATGAGAGAAGTTATAATGTGCGTTGCCGAGGATAGGGGGACAATGATTGTCGGTTGGTCCTGCGTACATGTAAGGTCGGTCGTACACATATTGTGGGTACCTGTCAGCAGCAACTCTAGCATAGGAAGTTTTGTCAGAAGGGATGTTGTTAGTGACACGGGGACCATATCCTACACCGTTAGCACGAGGTCTCTTTGGTTGAGGCTTTGTCGCTTCAGTTTCCCTTTTTTTGTCAGCTTTCGCCTTCTCTAGTTGAACAACTCGTTTCACGAGAGGTTCCAGAGGATACTGCTCTTCGAGTTTATGTTCTTCAATGCACTTGATTACAGCTTTATGAGCAATCAGCTCTCGTTCATTAACCTCAATCTGTGCAGTGGGGGATGAGTTACCCCTTTTGACAGGTGAGCAAGAATTTCTAGCATCCTTCAAATAAGATTTCAGAAGTGAAACAGGAGAAAACTGTTTTGTCAGATCAAATGCAAAAGCCAAGTTAACCGCGTCAATTTGCCTCCCACTGTTCACCAAAACCTCAATTACACCAGGCATCTTCTCTGATAATCCAAGGCAACGGCATAAATCAGCAGTTTGGCGACGCCGAGACACCATTGGAATTAGCCTGGATAACTCTTCCTCGTCAAAACCAGACGCAATATCAAAACTAGCTACGAGTTGCAAAAACGCATGAGCCTCCAAGGAATTCCCATTGCTAGCATCGATGTCAAGCGCATCCAATCTTGGCTTCCACTCTTCAGCAACTGCCTTTGCCCTATCCTTGATATCTTTGGAAATCACATTAGAAACGGAATGCAAATCGGTTAGGAAATCACTCAGACACTCCATCAGCATAATACAGGTCCGGCGAAGACCTAATAAGTTCGCATCTTTCTTAATATCCTGACTAGACACTTCCGTGCAGTAAAATCCTTCCAAAGAATCTAACACCAGACAGGCAGCATTAGGAGAAGCTCTTAACGCAAGTGGTATTTCCTCCCTTATAGCAGCAAGGTTCTTACGGTTATCAGATATGAATTTGTGAAGTCCAGAAACATCCATCTCTTTACAAAGTTTTACCAATTCTGGATAAGAGATCAATTCCACATTTCTATCTTCACGAGAAACTTTCACATCTTCTACGCGACCTTCCGTCACATTAGAAATAGCATCTGCATCCATCGGTTTCTCCTCCACACGTAATGCACATTGACCCCCATTGGAGACGATGCCCAAATCTTTTTGCAAAACCTTCCTGTGCTTTTCTCGAGCATTTACAATCGCAAATGTAGCAGCATCTCTTTTCTCTTGAAGCCTCTGCAATGTGGCTTGCTCCTGGGCAAAAATAGCCGCTTCCTGCTTCTTCAACATTTCACGAGCTTTCAGTGTTTTGTTCTCAAACACTTTTTCTTGATCTTCTAATTCGTGAAAGCGCCTCTTCAAGGATTTCTCAAGCCCGTGAAAATGTTCCTCCAGTTCTTTCCATTTCATGTTAAGAGTAACCGCTCGATAACTTTCGAGTTCAGCAAACGCCTTCTGCAGCTGTTGTATCTTCGAAGACGTAGAATCCATCAGTGTAGCAACCGAGTTTGTTTCTTCCATTGCTAAGACGCTGATTGATCTAGAAAAGAAACTAGTACTAGTAAAAACAAAGAGGAGGGAGAGGGAGAGATAGAGAGAAAGAGAGAGAGAGTTCTCAATCCATATT
TARGET=1814,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00439 Ps_contig_mira-and-tgicl-ass_franssen_4609
SEQUENCE=ACGCAACAAAAATTCATTGATTATTAAACATCCACTACATCTTACAAGACCTACAACAAACATCAAGAAATTAACATACTATCCTGCATATCCTTCTCATCCATAAGAACAAAAAAATATATACAGAAGCCTAAAAAAAATAGAAAACCATATTACATACTATAGTATATTTATCATTAATTCATAGGTTAATGAAATTCAAGAGCCCTATACTATACTCAAGATTATTATTATTAATTAAAACACTTGAAATCAAATCATTCAACAGGAAGGAACGACGCCGTATCCATCATAAGACTCATCCTCACCGATTAGATTCACATTCATATTCAAATCAGTGCGTTTTGCGAACCTTCCTTTAATTCTAGGTCTAGTTTCTGCATACGCTTTTCTCGAAGCGTAACGAATTGTCTTCTCGAACCTTCTATTCTTTCTCTTCTCTCTGTACCTCATAACCCTAGCTTCTCTATCCGCCGCCGTGATTTGCACCGCCTCCGTCCTTCCGTAACCGCAGTATGACATCTCCGACATCACGTTTCCGTCTGGTACAACTCCAACCTCCAGCGAAGGTGACGATACGCTGTGATTGTAGTTGTAAGTAAAAGGTTTAGAAGTAGAGAAATCGTTGTTGATATCGGAGTAGTGTTCGGTGACAGTTTTGCTATGAGACTGCACCGGCACAACTCCGTCTGCGGTGCCGGGACTATGTTGCTCCGGCGAGTCAGTTTTGGCGTCAACACAGACGAAATTGAGGTCGGTAGCGTCGATTTCGGTGAATGAGTAGTGAGATGAGTTGAGATCGGGGCCTTTAGGGTTGGCGGGAAGTTTGGAGGAGCCAGGAAGCAGCTTCGGCTTCTTCGGTGCTGACGTCAGCATCTGGATCGGATTCGGAGAAGAATTGTTGCTTGCTGTTGTGGTGGAAAGTGTTGAGCGGGATGCGTTCGTGGCGGCTAGCGRGGGGATTGGCGGAGTGGATGTCGTGGTCGCAGGAGACGCAGAGAGCAGCGGCGTCTGCTTTGCATGTGACGTGAGCAGGAGCTTGTTCACAGACTTCGCAGAGTGTGACGCGGTGATGGCGGGAGGCGAGTTTGTTGGCGGCSTGGATGTTGGAGTCACAGGTGATACAGAGGAAAGCTGAGTCAGAACGGCAGAATAGTGTTGCTTTTGTTGACTTGCAGGAATCACAGAGCTTAGTGGCCATTTGTTGTTTATTGAGTTAGTTAGAAGTTTGAGAAGTTGTGAGTGTATTGGCGAAGTGTTTTGGTTGGATTG
TARGET=224,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00440 Ps_contig_mira-and-tgicl-ass_franssen_4628
SEQUENCE=TACGGCGGGTTTGGTAAATCAAGTAGTCTCCGGCCTTCGGTTCCTAGACCAAATTACGACGAATTCAAACGAAAGGAAGGAAGGAGAAAACAAAATAGGTGGAAATTACTTCAACTGGTTCTTCTTTTTCTATAAGCATAGGTTCATTAACAGCGGTGACAGTTCTGGTTTACATTCAAGATCATGTGGGAAGAGATTGGGGATAYGGTTTGTGTGCGTGTGCTATTGTTGTGGCGCTTCTTGTGTTCTTGTCGGGGACAAAGAGGTATCGGTTTAAGAAACTTGGTGGGGAGTCCTTTGACTCAGATTGGTCGGGTTGGGTTTTTTGGTGGGCGGCGTGGAGGAAGGAGGGAGGGTTGGAGTTGCCGTATGATTCGTCGTTGTTGTATAATTTGGATGATGTTGAAGNTCATGATACTGAGACCACGCAAACGG
TARGET=73,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00441 Ps_contig_mira-and-tgicl-ass_franssen_4633
SEQUENCE=TTTCTACAAAGTCCTACATTTTGAGTTAAAATATTTTAGCACACAAGCAAGCTCAATTATTATTCTGTCTTTTGTAGCCATGTGCTAATTTTTTGTTATGATTCCTCGTGTATTGCTTTTCAAAGCAACTTCATCCATATGGCCTGAATTAATTTATAATAATCTCCTACAATTCATTAATATGGCCTGTGTTAATTTCTAATAATCTCCAACAATTGTATTTTCTGCTTTGCCATGAAAAATCCAATTCATATCTTGTTGCAGAGAAAAGAAGAATATGACATGGGTGGAACTAGAGGGCAAAAGCTTTCTGGAATGCAAAAGCAAGTACTTAGTTTATACAGAGGRTTTCTACGTGCAGCACGTTCCAAATCTGAAGAGGAACGCTGCAAGATAGAATCTGTAATATGTCAAGAGTTTCGATGCAATTCCAAGGAAGTGGATAGGAAGAATTTTCAATACATTGAATATTTACTTCGTCGAGGATATAAACAGCTTGATCAACTTCGGAACCCTGGTACCACTGGCTTATCTTTCTTTGACTAAAACTTAGTAATTTTTACAAAAGTTAAAACTTTAAAATTCTTTTCAATAAAGNTTCTTGTCTAATTTGTTATCCAATTAATTAATTCAGGTA
TARGET=620,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00442 Ps_contig_mira-and-tgicl-ass_franssen_4664
SEQUENCE=ATCTACTACCCACTTTCTAACTTTTAATTACAATAACAAACAACCAAAAAATAAAAATACAACCAAACCAACCAACCCTACCTTGGCCGAAACCCAAAACTACCACAAACCTAATCTCTCTGGCCATCCCACTTCCACCTTATATCTGTAGACTTGAGTACAGTGGAGCAAACCAAAAGCAAACCAAATCCATTTTCTATTGAGTTTCTCTGTTTCCTCAAACTCTACCAAATATACAATAATAATAACAACAACAAAAAAACATTTTTTTATATAAACCTCAAACTCTGCACAAACCATGAAAAATCTCTCTACTGTTCTGAACTTTGAGAATCATCCTTAGTAATATTCTGATTCTGGTTCTGATTTTGCTGATGCACCCTAGTTTGATGATTTTGACCAAACTGCTGATAAATCTGACTCAAAGCCTGGTTATTCAACACACCAATATGTCCATCTTGTGACAAACCTAATTCCAAACCAGGTGGCATCTGTTGATTCCCACCCAAAATTGATGTAAAACTCATCCCAACCCAAATTACGCCCCTGGACATATCAAATCCAGAAAACCCTAATTTTCTAGAAAATAACTTGAA
TARGET=66,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00443 Ps_contig_mira-and-tgicl-ass_franssen_4677
SEQUENCE=ATTGTTAGTAAATTTTGGATGTAACAAATACCCTCCCAAGTTTACTCCCCGAAAGTGACGCGTGTACTTCAGAAAGTGAAGCGTCACTAGTTGAAAATTCCATGTCCGGGATTCGACTGTATGCTCCGGCTTTATCAATTAGAATAGTTCCAATTTTTCACCATTACCTCATTCTTGTATGCATTTATTGATATTGAAATGTCTGGCCTTTGGTGTAATGGTAACTTTTGCGCAAGTTCAGCTACTGGTTCATGTTTCTCTCTTTTTCCGGACTTGTGCGAGCGCATGAGTATTTGACTGGTTCTATAGTACACGGCAGTTCAAGGTGGTTTATGTGCACAAAGAGAATGGTCTCACTCAAACCAGCCAATAAAGCTTAAATATGTAATGACCTTGAAATTCAACTCATTCTTCCGTTTATGTTACTGGAATATATATATTTTGTAATATGATCATTAACTGGMTAAGAGATCGAGT
TARGET=431,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00444 Ps_contig_mira-and-tgicl-ass_franssen_4689
SEQUENCE=TTTCAGAATTAACAACAGAAAAGCTGAATTCAGATTTAACAGCAGAAGAACCATTGAGGAGCATAAGTATAAGTTGATTTAAGGGTGCATCAATGTTTTCTTTGTTTTGAAGTTGAGTGAGTGAGCTTTGTTTTGCTTTTGTTTATTGGAAATTAGAGTGATTAGGGTTTTAGACTGCTGTGGTTTAATATGATTGTTTTAATAAGAAATGTTTGAGTTTTTGTGTAGAAAATTATTATTGCATTTTATGTTAAAATATATACGTCGGTTTAGTTTTTGTTTTA
TARGET=114,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0652
PRIMER_LEFT_PENALTY=0.629991
PRIMER_RIGHT_PENALTY=0.435201
PRIMER_LEFT_SEQUENCE=CAGAAGAACCATTGAGGAGCAT
PRIMER_RIGHT_SEQUENCE=AAACCACAGCAGTCTAAAACCC
PRIMER_LEFT=42,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=60.630
PRIMER_RIGHT_TM=59.565
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=1.0959
PRIMER_LEFT_1_PENALTY=0.660713
PRIMER_RIGHT_1_PENALTY=0.435201
PRIMER_LEFT_1_SEQUENCE=AACAGCAGAAGAACCATTGAGG
PRIMER_RIGHT_1_SEQUENCE=AAACCACAGCAGTCTAAAACCC
PRIMER_LEFT_1=37,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=60.661
PRIMER_RIGHT_1_TM=59.565
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=1.4181
PRIMER_LEFT_2_PENALTY=0.629991
PRIMER_RIGHT_2_PENALTY=0.788102
PRIMER_LEFT_2_SEQUENCE=CAGAAGAACCATTGAGGAGCAT
PRIMER_RIGHT_2_SEQUENCE=AACCACAGCAGTCTAAAACCCT
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=184,22
PRIMER_LEFT_2_TM=60.630
PRIMER_RIGHT_2_TM=59.212
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=143
PRIMER_PAIR_PENALTY_3=1.4488
PRIMER_LEFT_3_PENALTY=0.660713
PRIMER_RIGHT_3_PENALTY=0.788102
PRIMER_LEFT_3_SEQUENCE=AACAGCAGAAGAACCATTGAGG
PRIMER_RIGHT_3_SEQUENCE=AACCACAGCAGTCTAAAACCCT
PRIMER_LEFT_3=37,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=60.661
PRIMER_RIGHT_3_TM=59.212
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=1.6154
PRIMER_LEFT_4_PENALTY=1.180219
PRIMER_RIGHT_4_PENALTY=0.435201
PRIMER_LEFT_4_SEQUENCE=ACAGCAGAAGAACCATTGAGGA
PRIMER_RIGHT_4_SEQUENCE=AAACCACAGCAGTCTAAAACCC
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=61.180
PRIMER_RIGHT_4_TM=59.565
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_00445 Ps_contig_mira-and-tgicl-ass_franssen_4699
SEQUENCE=TTCCAAGGCGGAAGAGCTTGGTTTACTCAGCTTATTGGAGAAATTCGCCACTGTTTCTCCTTCTGTTTTGGCTTCTCTTTCGCTTCCTGTGCTTGTTGCTGCTGTTGCCGCGGTTGTTCTGATTCCGGATGATTCTACTGCTCTGGTTGTAGTTCAGGCCGTCGTGGCGGCGGTTCTTGGTGTCGGCGCTGTTGGTTTGTTCGCTGGGTCGGTTGTGCTAGGTGGATTGCAAGAGGCTGATTGATTGGTTCAGTTATTTTAAACGTTTTTTACTGTTGGTTTGTTTTTATGTTAATTACATTGTAGTAATTACTTTACTTCTATTTGTTTTAATACTAAAATTAATTAATTTTTAATTTTAACTTAAAAGAAAAAAACT
TARGET=340,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00446 Ps_contig_mira-and-tgicl-ass_franssen_4703
SEQUENCE=AAAGACTTTCTTATNTGCTGCTGATTTGTTTGTTTGGTTTTTGTTGGATTGTTCCTTCAGAATTAGAGCTAGTTTGAGTGTTGGGATAGTGTGGATGCGAATGCGCATTTCTTGCTTAGTTTGATAGATATATATGATATTTTATATGATTTTTCCTAATAATCTGGAGATTTATTTGAAGTTTTATGTTTACTCAAAAACAAGATTAAAGTTTATATTAACAGACCATACYAATAAATTTTTTATTGTTGTTTCG
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00447 Ps_contig_mira-and-tgicl-ass_franssen_4707
SEQUENCE=GGGGGATGGATTGAGTTAAAGTGTGTGTACAAACAAAGTCAAGTCTAGAGCTTCTTTCTTTCTACATTAGCACCTATAGTTCTTTTCTCTGTGAAACTTCAAAACATGGAGAAGAAAATGAGTTGGACAGTTGGAGATGCTGTGGACTATAAAGGCTTCCCTGCTGATAGGTCCAAAACTGGTGGTTGGGTTCCAGCTGCTCTTATTTTAGGGATTGAAATTGTTGAGAGGCTCTCTACAATGGGGATTGCAGTGAACTTGGTGACATATCTGATGGGAGTCATGCATCTTCCAAGCTCAACAGCAGCCAATACCGTGACCGATTTCATGGGCACGTCGTTTCTCCTTTGTTTGCTTGGAGGTTTTCTAGCAGATTCCTTCCTTGGAAGATTCAAGACTATTGGAATCTTTGCTGCAATACAAACACTGGGTACTGCAGCATTAGCAATCTCAACAAAATTGCCACGGCTAAGTCCACCACCTTGTCACGCGGGCGAAATTTGCAAACCGGCTAATGGAATCCAAATGGGAATCTTATACTTGGCTCTATACCTTATTGCACTAGGAACCGGTGG
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3728
PRIMER_LEFT_PENALTY=0.296177
PRIMER_RIGHT_PENALTY=0.076597
PRIMER_LEFT_SEQUENCE=GGGGATGGATTGAGTTAAAGTG
PRIMER_RIGHT_SEQUENCE=GATGCATGACTCCCATCAGATA
PRIMER_LEFT=1,22
PRIMER_RIGHT=288,22
PRIMER_LEFT_TM=59.704
PRIMER_RIGHT_TM=59.923
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.4109
PRIMER_LEFT_1_PENALTY=0.296177
PRIMER_RIGHT_1_PENALTY=0.114715
PRIMER_LEFT_1_SEQUENCE=GGGGATGGATTGAGTTAAAGTG
PRIMER_RIGHT_1_SEQUENCE=AGCAGGGAAGCCTTTATAGTCC
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=164,22
PRIMER_LEFT_1_TM=59.704
PRIMER_RIGHT_1_TM=60.115
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.4178
PRIMER_LEFT_2_PENALTY=0.296177
PRIMER_RIGHT_2_PENALTY=0.121635
PRIMER_LEFT_2_SEQUENCE=GGGGATGGATTGAGTTAAAGTG
PRIMER_RIGHT_2_SEQUENCE=CCCTAAAATAAGAGCAGCTGGA
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=59.704
PRIMER_RIGHT_2_TM=59.878
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.4401
PRIMER_LEFT_3_PENALTY=0.296177
PRIMER_RIGHT_3_PENALTY=0.143968
PRIMER_LEFT_3_SEQUENCE=GGGGATGGATTGAGTTAAAGTG
PRIMER_RIGHT_3_SEQUENCE=CAACCACCAGTTTTGGACCTAT
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=187,22
PRIMER_LEFT_3_TM=59.704
PRIMER_RIGHT_3_TM=60.144
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.4828
PRIMER_LEFT_4_PENALTY=0.296177
PRIMER_RIGHT_4_PENALTY=0.186600
PRIMER_LEFT_4_SEQUENCE=GGGGATGGATTGAGTTAAAGTG
PRIMER_RIGHT_4_SEQUENCE=TCCCATCAGATATGTCACCAAG
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=278,22
PRIMER_LEFT_4_TM=59.704
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_00448 Ps_contig_mira-and-tgicl-ass_franssen_4720
SEQUENCE=GATTTGGAGTTGAATTAGATCTTCCGTGATTGTGAGAGTGATTCATTCTTAGTATACACTCCCACTGCAAAATACAAAGAAAGAAGAAGAAGGATCTGTTTGGATCCAACGATGAAGTATTTTGGGTTTATTTTTTGTCTTGTGTTATTGATCCACTCTTGCCGCCGCCGCCGAACCATTAACTGTGGATGGGAAAGGTGTTGATACTCGACGAGTCCAATTTCGATTCCCGCCTATTTC
TARGET=81,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1622
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.129818
PRIMER_LEFT_SEQUENCE=CACTCCCACTGCAAAATACAAA
PRIMER_RIGHT_SEQUENCE=GTCGAGTATCAACACCTTTCCC
PRIMER_LEFT=56,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=0.2277
PRIMER_LEFT_1_PENALTY=0.118777
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=TCCGTGATTGTGAGAGTGATTC
PRIMER_RIGHT_1_SEQUENCE=ACCCAAAATACTTCATCGTTGG
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=126,22
PRIMER_LEFT_1_TM=60.119
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=0.2486
PRIMER_LEFT_2_PENALTY=0.118777
PRIMER_RIGHT_2_PENALTY=0.129818
PRIMER_LEFT_2_SEQUENCE=TCCGTGATTGTGAGAGTGATTC
PRIMER_RIGHT_2_SEQUENCE=GTCGAGTATCAACACCTTTCCC
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=60.119
PRIMER_RIGHT_2_TM=59.870
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.2739
PRIMER_LEFT_3_PENALTY=0.032347
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=CACTCCCACTGCAAAATACAAA
PRIMER_RIGHT_3_SEQUENCE=CGAGTATCAACACCTTTCCCAT
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=209,22
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.3603
PRIMER_LEFT_4_PENALTY=0.118777
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=TCCGTGATTGTGAGAGTGATTC
PRIMER_RIGHT_4_SEQUENCE=CGAGTATCAACACCTTTCCCAT
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=209,22
PRIMER_LEFT_4_TM=60.119
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_00449 Ps_contig_mira-and-tgicl-ass_franssen_4720
SEQUENCE=GATTTGGAGTTGAATTAGATCTTCCGTGATTGTGAGAGTGATTCATTCTTAGTATACACTCCCACTGCAAAATACAAAGAAAGAAGAAGAAGGATCTGTTTGGATCCAACGATGAAGTATTTTGGGTTTATTTTTTGTCTTGTGTTATTGATCCACTCTTGCCGCCGCCGCCGAACCATTAACTGTGGATGGGAAAGGTGTTGATACTCGACGAGTCCAATTTCGATTCCCGCCTATTTC
TARGET=161,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1622
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.129818
PRIMER_LEFT_SEQUENCE=CACTCCCACTGCAAAATACAAA
PRIMER_RIGHT_SEQUENCE=GTCGAGTATCAACACCTTTCCC
PRIMER_LEFT=56,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=0.2387
PRIMER_LEFT_1_PENALTY=0.108929
PRIMER_RIGHT_1_PENALTY=0.129818
PRIMER_LEFT_1_SEQUENCE=CCAACGATGAAGTATTTTGGGT
PRIMER_RIGHT_1_SEQUENCE=GTCGAGTATCAACACCTTTCCC
PRIMER_LEFT_1=105,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=60.109
PRIMER_RIGHT_1_TM=59.870
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=107
PRIMER_PAIR_PENALTY_2=0.2486
PRIMER_LEFT_2_PENALTY=0.118777
PRIMER_RIGHT_2_PENALTY=0.129818
PRIMER_LEFT_2_SEQUENCE=TCCGTGATTGTGAGAGTGATTC
PRIMER_RIGHT_2_SEQUENCE=GTCGAGTATCAACACCTTTCCC
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=60.119
PRIMER_RIGHT_2_TM=59.870
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.2739
PRIMER_LEFT_3_PENALTY=0.032347
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=CACTCCCACTGCAAAATACAAA
PRIMER_RIGHT_3_SEQUENCE=CGAGTATCAACACCTTTCCCAT
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=209,22
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.3505
PRIMER_LEFT_4_PENALTY=0.108929
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=CCAACGATGAAGTATTTTGGGT
PRIMER_RIGHT_4_SEQUENCE=CGAGTATCAACACCTTTCCCAT
PRIMER_LEFT_4=105,22
PRIMER_RIGHT_4=209,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00450 Ps_contig_mira-and-tgicl-ass_franssen_4724
SEQUENCE=AAAAATTWACGATAAGCTTATGGATCTTAATATTATATATATACTACTGACCAATGTTTTTGGGTGAATACACCATTTACAAAATCAATTTAAAAATAAAACAATATACACTGGCAAGTCTTTTTAAAATAAAACTCTAAATTCCACAACATTACCTCGTATGCAATTATAAATCATATTTTAGAAGAAAATATCTGATTGTTTTGACTGGGCTTTAAGATCCTTCAATTTCTTCAATATGTTTGTGAAGTAGTCCTGCAGATACTTTTCCAATGTAATTATATCTTTTGTATCCACACCTAGCAGATTGTATGTCTCCCCCATCGGAACAGAGAAAATTGTGTCACTCGTAAGTACCTCTGAAAATGCCAATCTGTCAGCAACATCATTTGTCCACTCAAAAAACCTAGTTAACTGGCGAGTAAATCTTAAAACAGAGACAGGAACTGTAGTTACATTAGCATCTTGACCAGCAAGCCTCTCACACAATGTTATCACCTCTTGGGTTGTCCATGCACGAGGACCGGCAAAAGTAAGAAGTTTACCATTGATTTTTTCATTTCGCAATGCTATGAAAGTCAAGCGAGCTATATCCTGAGTATCCATGTAAGCAATTCTGGTGGGAGCATCAGTTCCCCAAACAGACTTTTCTTCCAGGATAGGAACTGCATACTGGCCTATAAGACCTTGCATGAAACCACATAGGCGAATGACAACGTGATTGAGTCCGGAATCACGGAGGAAGTTCTCGGTGCAATACTTGATCTCCATGAGTGGAACCTCGGGATGTTTGTCGCAGTTATGGATGGAATAGAAAACATATTTCTGAATTCCCATAGCTTTGGCACATTGTATAAGAGCCACTTTACCTTCCCAGTCTACAGTTTTGATGGGCTCTTCAGGACGGCCAGTGGCGCAGTCAATAATAGTATGAACGCCAACCAAAGTAGCGGGAATAGTCTCTGGTTTACTAAGATCGCCATTGACAACAGTTGCTCCCCAATCACGAAGGAAGTCAGCAGGAGCGGGCCTCGGCCTTACAAGGCACCTGACGTCGTATCCTTCGTCGAGTGCTCTTCTCACAATCTGCCTTCCGAGAGTGCCGGTGGCTCCCACAACAAGTATGTTGGTTGACCTAACAGGTGTCCCGGTCCCAAGATTCACGGCGGATCTATCACCAACATCTCCACTAATACTACATTTCACACCACCGACTCTCCTATTACTACTTCCATAAACGTCAAGGTGAAATGCATGTCTCCTCGTGGAGGGTGCTAGTGCTACACCCCATAGTCTCACCCTAGAACCAGTAGCAGTAGAAGTGGGTAGCCTCAAACCCGCCATTTTCATATCACCGGGAAATGCTTCGTTCTTCTCTTTCTAATTCTCATTCAATCCGATACTCATGATCGTAATTTCGCCACGTCTTCCATTGCTACCACACACCGCC
TARGET=34,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00451 Ps_contig_mira-and-tgicl-ass_franssen_4725
SEQUENCE=AAGATATAATTACATTGGAAAAGTATCTGTCTAGGACTACTTCACAAACATATTGAAGAAATTGAAGGATCTTAAAGCCCAGACAAAACAATCAGATATTTTCTTCTAAAATATGATTTATAATTGCATACGGGGTGATGTTGTGGAATTTAGAGTTTTATTTTAAAAAGCTTGCCAGTGTATATTGTTTTATTTTAAATTGATTTTGTAAATGGTGTATTCACCAAAANTTGGTCAGTATGTATATATATAATATTMAGTWCCWTAAGACTT
TARGET=243,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00452 Ps_contig_mira-and-tgicl-ass_franssen_4730
SEQUENCE=AGGTTAGCCAGCAAAGAGGATTCCCAGGTGGCAGGTGACGATTGAACAATTAAGAACCAAACACAGCTCCTCCAAAAGCATCTTGAAAACTAGAAACACGCAAAGAATGCATGTTTTTGGGGATTCGGTTGTGAAGAACAAGAGGGTTTGGAAATCGAGTTATTAAATTGGGTTTTGGAGAGAGAGAAATGGGTTTGCGTGGGAAGTGAAAAGGAAGAGAAAGATACGTGATYGAGTGAAGAGTTAATTGCGCCATAATGTGTCTGTCGATGTCAACGTGAAGGGAAAAAAGATGAGAATGAAAAGAAA
TARGET=178,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00453 Ps_contig_mira-and-tgicl-ass_franssen_4757
SEQUENCE=GATAGAGAGTGTGACTAAAGAATCSGTGTTCGAATCGAAGAGAGCGCAAACTAAACACGGAGCTATTTATTTATTCCTTCTTTAACGCCCTTTTGACTTCACACCCTTCGCATTCTCCGCCACCTTCTCAACCCTCTCTTCTCTTTTTCACTCTCTTTCTCTTCGTCTCTCCACACAAACAACACCACCAACCTCCATGGCGATCAATATGCAAGCATCACACTCTGTGCTCTCTCTCCGTCCTCAACTCAATCAGCGACTGGCTGTCACTTCAACCGCCGGCCAAAGGTCTTCCTTAATTTCAAGCTATGGGAGATGGAATGTGTCTTTTAGCCCAATGTCACGCCCAATGCGCCTTACTGTTACCTGTGCTGCTAAACCACAGACAGTGGAAAAAGTGAGCCAGATAGTGAAGAAGCAGCTGGCTTTATCAGATGACTCTAATGTGACCGGCGAATCAAAATTTGCAGCTCTTGGAGCTGATTCGCTTGACACGGTTGAGATTGTGATGGGACTCGAGGAAGAGTTTGGCATCAGCGTTGAAGAGGATAGTGCTCAGAGCATCACAACAGTTCAAGAAGCTGCTGATCTTATCGAGGATATCCTGAGTAAACAGAGTCCTTAGACGAAAAATGTTGCAGATGTAAAACGCCTCTGAAAGACATGCATAGTTCTTATTCATACTGTTTTTTAGAATGTGGTGGTCAAGTCTCTAGTGTTTTATCTGCATAGTTTGCTACTGTCTTGTTTCGTAGGTCTTAAAGAGCAAATTAGCGAGCTACTTGTGTTTTGGAGAAAATGGCTGTGTAGTCCGTTCTTACTTTCTTTGCTCATTAAGGGCTTATCAAATTGTTTTAGTTACTTTAAGTTGTTGAGCATGCAATCCATAAATTTGCTGCATAATAATGAATAAGAGACTAAGTTAAGGGTAAATAAAAAATTTAAAAAAAAAACG
TARGET=64,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00454 Ps_contig_mira-and-tgicl-ass_franssen_4776
SEQUENCE=AAAAGTAAAAAGAAGAAAGTAAAAGTAAAGTAGTGAATTCATCAAGAACAACCGGTTAATATTCCTAATTGGTCAAAGATTTATGGTCATGATGATGATAAGGTAAACAAGAGTGTTAAGAATGTTTCAAGGTATGGTAATGATAATTATGGTTACTATGATGATCATGAAGGTGGTGATGATCATGAAGTTTTGAACCATGGCGAAAGAGGTAGCGATGATGATGATGATGATGGTGAAGATGAAGATGAAGATGATGATGGTGATGGTGAGTATGGTACTAGGCTGCCACCTCATGAGATAATTGCGAGAAGGCTTGCAAGGAGTCAGATTTCTTCATTTTCTGTTTTTGAAGGTGTTGGTAGGACACTTAAGGGTAGGGATCTTAGCAAAGTGAGGAACTCTGTCCTCATAAAGACTGGTTTTCTTGAATCATTATGATTAAGGGTTTTTCAATTGATCAAATTTGTGAGGTTTTAAATTACGGTTGCGAACTTTTGTATTGCATTAAATTTTGAGAATTTGAAATTGCAGTTGTAATATAGTTGCAAGGACTTCTCAATTA
TARGET=217,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2808
PRIMER_LEFT_PENALTY=0.203457
PRIMER_RIGHT_PENALTY=0.077340
PRIMER_LEFT_SEQUENCE=GATGATCATGAAGGTGGTGATG
PRIMER_RIGHT_SEQUENCE=GGACAGAGTTCCTCACTTTGCT
PRIMER_LEFT=159,22
PRIMER_RIGHT=408,22
PRIMER_LEFT_TM=60.203
PRIMER_RIGHT_TM=59.923
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=0.2808
PRIMER_LEFT_1_PENALTY=0.203457
PRIMER_RIGHT_1_PENALTY=0.077340
PRIMER_LEFT_1_SEQUENCE=GATGATCATGAAGGTGGTGATG
PRIMER_RIGHT_1_SEQUENCE=AGGACAGAGTTCCTCACTTTGC
PRIMER_LEFT_1=159,22
PRIMER_RIGHT_1=409,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=59.923
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=0.2808
PRIMER_LEFT_2_PENALTY=0.203457
PRIMER_RIGHT_2_PENALTY=0.077340
PRIMER_LEFT_2_SEQUENCE=TGATGATCATGAAGGTGGTGAT
PRIMER_RIGHT_2_SEQUENCE=GGACAGAGTTCCTCACTTTGCT
PRIMER_LEFT_2=158,22
PRIMER_RIGHT_2=408,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=59.923
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.2808
PRIMER_LEFT_3_PENALTY=0.203457
PRIMER_RIGHT_3_PENALTY=0.077340
PRIMER_LEFT_3_SEQUENCE=ATGATCATGAAGGTGGTGATGA
PRIMER_RIGHT_3_SEQUENCE=GGACAGAGTTCCTCACTTTGCT
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=408,22
PRIMER_LEFT_3_TM=60.203
PRIMER_RIGHT_3_TM=59.923
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.2808
PRIMER_LEFT_4_PENALTY=0.203457
PRIMER_RIGHT_4_PENALTY=0.077340
PRIMER_LEFT_4_SEQUENCE=ATGATCATGAAGGTGGTGATGA
PRIMER_RIGHT_4_SEQUENCE=AGGACAGAGTTCCTCACTTTGC
PRIMER_LEFT_4=160,22
PRIMER_RIGHT_4=409,22
PRIMER_LEFT_4_TM=60.203
PRIMER_RIGHT_4_TM=59.923
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=250
=
PRIMER_SEQUENCE_ID=SSR_00455 Ps_contig_mira-and-tgicl-ass_franssen_4788
SEQUENCE=AGTGGTACATATTGTCCGAAGAACCATGTGKMDGGGAAGGTTCATTCATTTTGTTCCTCCAACGTATGCTTCTCACTAACATCATCAACAAACCAGTGACAGAAAATGGAATAAAAAATAACAAGAAAAAAACACAGGAAGAATCATAATGCAAATGGCACTTTTGTTAAGAGGTGGAGCACTTGGAGATTCTACCTTTCGTTTGTGTTCTTTAACTTCAACCTCATGTTCTTTACATGTCTCCCAAAATGTTGTCATACCGAACTCATCGTCATCACCAATACTACCTTTGATTGCAAGTAGATTTAAAACGGTAAATAGAAACAAGATAATTTGCTCTGCAGTTCAAGAATCATCTACTACTAGTACATCAGCTACTGCTGAAACAAAGGGGGAGGTAAAGGCAGCAGCAGCAGCAGCTCCAAAAGCAGCAACAGGGAAGAAGGCTCCACCTGCTAAAGCTCCGGTTAAGCCTTTACCTCAGATGATGGAAGAGGATGTGATTCCTTCGTTGAAAACAATTTTGGAATCCAATAAGGATCTCTCTGATATTGAGTTGGTTTTTCAGGACAATAAGTTGGAAGGTTCATTTCTGAAAAAAGGCAATCCCTATTCATTTTGGGCCTTTTTTCCCACAGGAATTACTGGTCCAAAGGGGTTTTCACTGTCATCTTATAACTCAGGAGCAAGCTACGGTTGAACCGATTCTCATTGATGAGAAGAAATCNCTTCAAAACACATAGTTNTTTTGGGTTAGAAAAAACGGTTTTGGGCGAGACGAACGAGGGGGGTAATTTATTCCTGTAGTGGAAAG
TARGET=404,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00456 Ps_contig_mira-and-tgicl-ass_franssen_4811
SEQUENCE=AGGCATGGATTTAGGGTTTGACCACGAATAGAAAGATGATTTTCTTGAAAATTTGGATGCAATTAGAACCCTTCTTCTCTTGTTAAAAGGACTCTCTTGCTTCCGCAACTCGGTTAAATTGCTTACTTGTGATAGATCTGTGAAGGTATTTTTGACTTTTTCCTTACAAAAATGACTCGATAACTCCCCTCTAATCACAAGATTAATACAAAAATTAGAAAAATTAAAACAAAAACAAGTAGAGAGAAGAAAGACAAAGAGAGAAGAGAGAGAGAGAAGAAGAGAACTAACG
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00457 Ps_contig_mira-and-tgicl-ass_franssen_4813
SEQUENCE=ACCTTTCKTCKSRKYCTYCRTYTGGTTCGTCTCCATAACCTTGCCCCGAACCCGAACCATAGCACCCGACCCAAACTCCTAACCTAACCTAACCTAACACCGTTACAGTTATGGGAACGGAGAGTTATACCTACATGGGACGGAGTTTCAACGAATTCACCATTAACGACGATTCTACTTCCTCCGCTTTCAGCGACTGTAACAGTGATAGATCCGGCGAATTCGCCACCACTTCTTCTCAATCCCCGACGACTCTATTATCGCCTGTGCTT
TARGET=78,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00458 Ps_contig_mira-and-tgicl-ass_franssen_4814
SEQUENCE=TGTTGATATTTGGGTGGAAAACTTTAGTCCTAAATGTCTAACCTTGGGTGGCTTGAATGGATAATCAGGAGGAAAATGAATGGTAACTAGAAAAACACCTCCAGCATAAGGGCTATCAGGGGGACCCATTATAGTTGCTTGCCAATGAAACATATCTTCTGCTACAGGGCCAGCACTGCATGAAGTTGGAGGATCTTTCTGAAGGTCCTTGAGTTCCTTCAGGATCCGCTTCGAGGCCATGGAAATACAGAACAAGATGTGAGAGAGAGAGAGAGAGAGGAGGTASAMCCTTAAACGTTTTAAACGTTAAAAAACGTAAAAGGAAAAAGGTAAAAAGGTAAAGGAAAAAAGAAAACGAACTAAAACGG
TARGET=261,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00459 Ps_contig_mira-and-tgicl-ass_franssen_4817
SEQUENCE=GGAAAACAGCCGCCAATAATAAACCAAACAGAAAAAACGCTGGGGGATTTTTTTTCATTTCTCATTCTCGTTCGTTCATTCCTTAGCGTTTTTTGTTGAATCCAATCGAAGAAGAAGAAGATGGGGAAAAATATTGTGCCGGTGAAATCTGTTGTGTATGCTCTTTCTCCTTTCCAGCAGAAAATCATGAGCGGTTTGTGGAAGGATTTGCCAAACCAAAATTCATCACAAGGTCTCCGAAAAATTGGATCAGCGCTATTCTCTTGTTACTCCCGTCGTCGCACTTACTCTATGCTA
TARGET=108,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3415
PRIMER_LEFT_PENALTY=0.328094
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=GTTCGTTCATTCCTTAGCGTTT
PRIMER_RIGHT_SEQUENCE=GCTGGAAAGGAGAAAGAGCATA
PRIMER_LEFT=69,22
PRIMER_RIGHT=177,22
PRIMER_LEFT_TM=59.672
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=0.4514
PRIMER_LEFT_1_PENALTY=0.328094
PRIMER_RIGHT_1_PENALTY=0.123278
PRIMER_LEFT_1_SEQUENCE=GTTCGTTCATTCCTTAGCGTTT
PRIMER_RIGHT_1_SEQUENCE=TTCTGCTGGAAAGGAGAAAGAG
PRIMER_LEFT_1=69,22
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=59.672
PRIMER_RIGHT_1_TM=60.123
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=0.5852
PRIMER_LEFT_2_PENALTY=0.571751
PRIMER_RIGHT_2_PENALTY=0.013401
PRIMER_LEFT_2_SEQUENCE=CCGCCAATAATAAACCAAACAG
PRIMER_RIGHT_2_SEQUENCE=GCTGGAAAGGAGAAAGAGCATA
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=177,22
PRIMER_LEFT_2_TM=60.572
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.5970
PRIMER_LEFT_3_PENALTY=0.328094
PRIMER_RIGHT_3_PENALTY=0.268895
PRIMER_LEFT_3_SEQUENCE=GTTCGTTCATTCCTTAGCGTTT
PRIMER_RIGHT_3_SEQUENCE=CGGGAGTAACAAGAGAATAGCG
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=274,22
PRIMER_LEFT_3_TM=59.672
PRIMER_RIGHT_3_TM=60.269
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=9.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.6011
PRIMER_LEFT_4_PENALTY=0.328094
PRIMER_RIGHT_4_PENALTY=0.273036
PRIMER_LEFT_4_SEQUENCE=GTTCGTTCATTCCTTAGCGTTT
PRIMER_RIGHT_4_SEQUENCE=GAGAATAGCGCTGATCCAATTT
PRIMER_LEFT_4=69,22
PRIMER_RIGHT_4=262,22
PRIMER_LEFT_4_TM=59.672
PRIMER_RIGHT_4_TM=59.727
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_00460 Ps_contig_mira-and-tgicl-ass_franssen_4826
SEQUENCE=CGYGTAGTAATAAATCACATGACCATATAGAGAATCAAAACTAACAAGAAAGATATTGTCACAACGACCCTGCAAGCATTCTTTAAATTATCGTGTGATAGACCATGGCAAAATAATTGATCATAAGAGTCTCAGGTTTCTTAAATAAATGACAAATACACCTGTTACTTATGCACTATTGGCTATCGTAAGAAAGGTCATTGAAATTGCGTAAAGTTAGGGGGTTGACTCAAGACTGATAAAATTGTGTGCATGGCATGTCCATGCTGCAGCAGCAGCAGCAGCAGCGCCTGTTGAGAGTTGAACACAATGAAGGGATACGGAGATACCCGTATAGTTTTATCTCACTTTCTTCTCTTTAGTAGCCTGAGGCTTTTGGTACATCACTATCTGACTTAAGATGGTAAAATTTGTCGCAACACCAATTGCATAGCCCAGTAGAACACTTTGGGGGGCATTTTCTTGGATGGTGGTGAATACTCTAACCATTGAACCTCCAAAGTTCATAAAAGAAGTTAAAAAGCTGAGCTCCCCTGTACTTTTATTAGAAAAGTTTTGCCATACTTGTGGGACCCTTGCACACAGAAATATTAAATGCTGGGATGCATACAAGGCCTCAAAGAGGATAGGATCAATTTTACCAGCTAAGACTGTGGGTGCTACAGCGCAATATATCAATGGCCGAATCCATGTGATTGTACTAATAGGTTGAGAATAGTAGTAGATTATGGCAACCAATACAAAAGCTTGGATCAAAAGAAACAACAATTCTCCATAAGCTGAAAAAGGAAGGCCTTTGTGAAGAGAGTAAGCCAATGCTATGGTATACCCAACAACTTCAAGCTCGAACGCTAACATGCTAAGGCCTCTCACACTTTTATGCTTCAAAATTTTCAAAATCTGAGGGAGTTTGACGGTGGTGGAAGCGGCGACGATGGCGTAACCGAGGAGTTTGGAAATGAGAGGAAGCAAGCAATCTTTGTCAGGGAAGTTTCCGTTACGAAGAGATCCAACGGCACAACTCAAATCAATCCCTAGATATTCCATCGTCTTCTCCGATCTCAATCTAAGGGTTTCTTTCAATGCAAAAAATCGCTTCTCTGGTTTGTTGTTGTTGTTGCCGTCAATGTTTACTCAAACTGAACTTTCTAAAATTCTCTTTTTTTCGTTGAATCMTCCCCGA
TARGET=269,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00461 Ps_contig_mira-and-tgicl-ass_franssen_4826
SEQUENCE=CGYGTAGTAATAAATCACATGACCATATAGAGAATCAAAACTAACAAGAAAGATATTGTCACAACGACCCTGCAAGCATTCTTTAAATTATCGTGTGATAGACCATGGCAAAATAATTGATCATAAGAGTCTCAGGTTTCTTAAATAAATGACAAATACACCTGTTACTTATGCACTATTGGCTATCGTAAGAAAGGTCATTGAAATTGCGTAAAGTTAGGGGGTTGACTCAAGACTGATAAAATTGTGTGCATGGCATGTCCATGCTGCAGCAGCAGCAGCAGCAGCGCCTGTTGAGAGTTGAACACAATGAAGGGATACGGAGATACCCGTATAGTTTTATCTCACTTTCTTCTCTTTAGTAGCCTGAGGCTTTTGGTACATCACTATCTGACTTAAGATGGTAAAATTTGTCGCAACACCAATTGCATAGCCCAGTAGAACACTTTGGGGGGCATTTTCTTGGATGGTGGTGAATACTCTAACCATTGAACCTCCAAAGTTCATAAAAGAAGTTAAAAAGCTGAGCTCCCCTGTACTTTTATTAGAAAAGTTTTGCCATACTTGTGGGACCCTTGCACACAGAAATATTAAATGCTGGGATGCATACAAGGCCTCAAAGAGGATAGGATCAATTTTACCAGCTAAGACTGTGGGTGCTACAGCGCAATATATCAATGGCCGAATCCATGTGATTGTACTAATAGGTTGAGAATAGTAGTAGATTATGGCAACCAATACAAAAGCTTGGATCAAAAGAAACAACAATTCTCCATAAGCTGAAAAAGGAAGGCCTTTGTGAAGAGAGTAAGCCAATGCTATGGTATACCCAACAACTTCAAGCTCGAACGCTAACATGCTAAGGCCTCTCACACTTTTATGCTTCAAAATTTTCAAAATCTGAGGGAGTTTGACGGTGGTGGAAGCGGCGACGATGGCGTAACCGAGGAGTTTGGAAATGAGAGGAAGCAAGCAATCTTTGTCAGGGAAGTTTCCGTTACGAAGAGATCCAACGGCACAACTCAAATCAATCCCTAGATATTCCATCGTCTTCTCCGATCTCAATCTAAGGGTTTCTTTCAATGCAAAAAATCGCTTCTCTGGTTTGTTGTTGTTGTTGCCGTCAATGTTTACTCAAACTGAACTTTCTAAAATTCTCTTTTTTTCGTTGAATCMTCCCCGA
TARGET=1106,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00462 Ps_contig_mira-and-tgicl-ass_franssen_4837
SEQUENCE=GAATCTCTCTTGCTCTTTCATCGCTTCTCTTCAATTTTATTTCAAAGTTGAAAGAAGCTATCATACTCTCATGGCGTCCGAAAATTCCAAACCAGAGGTCTCGTTAGAGAGTTCAAAGGTAGAAACAATAAGCAAAGCTGTAGATTCTCTTCTCAAATGGCACAAATACGACGTTTAAGAAAACCGAAAAACCTTAAACTTTTACGAAGAAGAAGAATTCATCTATCTGGTATTAACCCTAAAGAAGATTCCTAACAAATCGGCCGTGTTAACCCTCA
TARGET=206,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7510
PRIMER_LEFT_PENALTY=0.003166
PRIMER_RIGHT_PENALTY=0.747796
PRIMER_LEFT_SEQUENCE=TCTCAAATGGCACAAATACGAC
PRIMER_RIGHT_SEQUENCE=GTTAACACGGCCGATTTGTTAG
PRIMER_LEFT=150,22
PRIMER_RIGHT=272,22
PRIMER_LEFT_TM=60.003
PRIMER_RIGHT_TM=60.748
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=0.8715
PRIMER_LEFT_1_PENALTY=0.123706
PRIMER_RIGHT_1_PENALTY=0.747796
PRIMER_LEFT_1_SEQUENCE=ATCTCTCTTGCTCTTTCATCGC
PRIMER_RIGHT_1_SEQUENCE=GTTAACACGGCCGATTTGTTAG
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=60.124
PRIMER_RIGHT_1_TM=60.748
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.8000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=1.0098
PRIMER_LEFT_2_PENALTY=0.003166
PRIMER_RIGHT_2_PENALTY=1.006651
PRIMER_LEFT_2_SEQUENCE=TCTCAAATGGCACAAATACGAC
PRIMER_RIGHT_2_SEQUENCE=TTAACACGGCCGATTTGTTAG
PRIMER_LEFT_2=150,22
PRIMER_RIGHT_2=271,21
PRIMER_LEFT_2_TM=60.003
PRIMER_RIGHT_2_TM=60.007
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=1.0302
PRIMER_LEFT_3_PENALTY=0.282356
PRIMER_RIGHT_3_PENALTY=0.747796
PRIMER_LEFT_3_SEQUENCE=CTCTCTTGCTCTTTCATCGCTT
PRIMER_RIGHT_3_SEQUENCE=GTTAACACGGCCGATTTGTTAG
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=272,22
PRIMER_LEFT_3_TM=60.282
PRIMER_RIGHT_3_TM=60.748
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=1.0302
PRIMER_LEFT_4_PENALTY=0.282356
PRIMER_RIGHT_4_PENALTY=0.747796
PRIMER_LEFT_4_SEQUENCE=CTTGCTCTTTCATCGCTTCTCT
PRIMER_RIGHT_4_SEQUENCE=GTTAACACGGCCGATTTGTTAG
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=272,22
PRIMER_LEFT_4_TM=60.282
PRIMER_RIGHT_4_TM=60.748
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_00463 Ps_contig_mira-and-tgicl-ass_franssen_4838
SEQUENCE=RGATATGAATAATCGACGCGATTTATTTGCTCATGACACGAGCTTAGCTCATTCTTTTCTGTAGAATCATACACCAAGAGTCTCATCACTTATTTTCTTCATATAATACAGAAATCAATTAACTTATTTCCTCAAAATACATACTCCAACTATTTGCTAATTTCTGAACTTAATACATCAATTATAACTTACATGGTAAAAACGTCACGCAGTAAAGGAAAATTCAATTGAAACGCGCCACCAATATTGAATGACTCACCAGAGCTGAAACCATATTCTCTCAAGCAGCCAGCCTGTGATTTGTACTCTACTTGAGGAATTACATTTGTTTGTACAAAATGTTATTCGCCATCACTCAAGATGGGAACTATGAGGGGGTTCATTTGTGCCAGATATACAATTGCCCACATCATCCAGCAGCATATTGTTGAAGTAAGGACCAAAGTGAGGTGAAATAAATTAGCAGAAGGTCCTTTGTTGAAGCAAATTCTTGTAGAAAGGCATGCGATGATTCCGACGACAAGGAAGATAAGCGAGGTGACAACGAAACCCATATCGATCTCTGAAACGGTTTGTCGTTTATTGTATGAATGAATGATGGGAAGGTGAAGCTCCCAGGTGGGGTGTGTATTTTATTTTTTATTATTCTTATTTMAAATTTTGGTATTTTTGTGGTACCTTATACTC
TARGET=587,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00464 Ps_contig_mira-and-tgicl-ass_franssen_4839
SEQUENCE=GATCATTCATTCATACAATAAACGACAAACCGTTTCAGAGGTAACCTAATTCTCACGTCGTTTTGATTCTTCCCTCACTTTCTCGCAAATCAAATCAAAAACCGTAACTTTAACAGAACCATTTTCTTTCAATTTGTGTAATTCAGATCGATATGGGTTTCGTTGTCACCTCGCTTATCTTCCTTGTCGTCGGAATCATCGCATGCCTTTCTACAAGAATTTGCTTCAACAAAGGACCTT
TARGET=3,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00465 Ps_contig_mira-and-tgicl-ass_franssen_4841
SEQUENCE=ACGGGGGAGGAAATGNGATTGTTGAAGAAAAAGAAAGACTAGAGATGGTGCAAGATTTCTTCATTAGTTACTATGAGATGTTAAGATTTATGATTATGATGATGATGATAGTTGAAAAGTGGTTTGAAATGACGTGTTATAAGAGAAACCACATTTCTTAGGATCTTCTTTAGATTTCCCTTTAAAAATTATAACAATGTTGTTTTGGTGTTTTAAACTTATTTTTTATTTTCTTTGATCTTTGTGTTAGAGAGTTCATTTATTGTATGTGCGAGGTTTTGTTGAATGAAGATGAATATCTCTCCTTTATTTTCTCATAM
TARGET=96,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00466 Ps_contig_mira-and-tgicl-ass_franssen_4892
SEQUENCE=ACATGCCTGCTCAAACCATGACCCCTGAGCTGCAGAGAAATCTGAAGTTACTAAAGTTGAGGGATGCCATTGATCCAAAACGGCACTACAAGAAGGGTGATTCAAAATCAAAGAGTCTTCCAAAATACTTTCAGATCGGAACAGTTGTAGGTTCTCCGCTGGACTACTTCTCAGGCAGATTAACAAAGAAAGAAAGGAAAGAAACGTTTGCAGATGAGTTGCTTGCTGATCAAAAAGATTCAGCTTACAGGAAGCGTAAGGTTCGAGAAATCGAAGAGCAGAGGCAACCAGCTGGGAATGAAAAGTGGAAGATCAGAGGCCAGAGTTCTCGGAAACGTGCAAAAGAAAGGAGGAAGTTCTGAATTTTACAGACTTGTTGGTTGGAGTTTATTGCATTCAGTTTTGTTTACAAGTCTGGAAATTAATGCAATGGAACAATTTATAATGGTTATATTTAGTCGATTTATATAATAAGTCTAGTCAGTGTTTGGAGTCTCATTTTTGTATTATGGAAATTTATGGTCGTAATTTCTTTCAACTTTAGCATTTGTATCATAAGAATTAGTTATTGTTGTTTAAGCTCATTTTTAAAAAAATGAAGAAGTGAAACAATTTAAGAAGCTGAGTACGCATATTAT
TARGET=185,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0267
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.022302
PRIMER_LEFT_SEQUENCE=GGCACTACAAGAAGGGTGATTC
PRIMER_RIGHT_SEQUENCE=TTGATCAGCAAGCAACTCATCT
PRIMER_LEFT=81,22
PRIMER_RIGHT=232,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.022
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=0.2536
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.249232
PRIMER_LEFT_1_SEQUENCE=GGCACTACAAGAAGGGTGATTC
PRIMER_RIGHT_1_SEQUENCE=CTTCGATTTCTCGAACCTTACG
PRIMER_LEFT_1=81,22
PRIMER_RIGHT_1=275,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=60.249
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=0.3034
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.299089
PRIMER_LEFT_2_SEQUENCE=GGCACTACAAGAAGGGTGATTC
PRIMER_RIGHT_2_SEQUENCE=GATCTTCCACTTTTCATTCCCA
PRIMER_LEFT_2=81,22
PRIMER_RIGHT_2=313,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=60.299
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.3034
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.299089
PRIMER_LEFT_3_SEQUENCE=GGCACTACAAGAAGGGTGATTC
PRIMER_RIGHT_3_SEQUENCE=TGATCTTCCACTTTTCATTCCC
PRIMER_LEFT_3=81,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=60.299
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.3991
PRIMER_LEFT_4_PENALTY=0.004359
PRIMER_RIGHT_4_PENALTY=0.394711
PRIMER_LEFT_4_SEQUENCE=GGCACTACAAGAAGGGTGATTC
PRIMER_RIGHT_4_SEQUENCE=TTTGATCAGCAAGCAACTCATC
PRIMER_LEFT_4=81,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=60.004
PRIMER_RIGHT_4_TM=60.395
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_00467 Ps_contig_mira-and-tgicl-ass_franssen_4921
SEQUENCE=AACACGATTCTGACACTTATGATTATGTTTAATTTATTTATTTTTCAAATCATCATTGATATCAATGTGTCAGAATCGAAGTCGTGTCTGGTGTCCGTCATGATATCTATTCAATAAAAAAAAAGTATTACATGTTAGT
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00468 Ps_contig_mira-and-tgicl-ass_franssen_4923
SEQUENCE=TTTTTTTGTATGATTTGAAAAAATAAATAAATTAAACATAATCATAAGTGTCAGAATCGTGTTGATATCTGACGTTGACACGAACACACCCTTTTTTCCCT
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00469 Ps_contig_mira-and-tgicl-ass_franssen_4925
SEQUENCE=ACACACANTANATTNTCTAATAATAATAACACATAATTGGTCAACTCCATACCAGGANCTTTAAGAAATAAACAAATTACGTATCTTCTTGAACTGGATGTGGTTGTCTCAAATTTGTATTATTATTRTTAGAGAAATRTAACATGTTGGTGTTGAAACTTGTGACACATGAATTAAGTTTCTTCTATTTTTTTCATTATGTTTATAAAATATTTTTAGAAAACACCA
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00470 Ps_contig_mira-and-tgicl-ass_franssen_4927
SEQUENCE=CTCTCTCTCTCTATAAGATACGCAGCATGCAACAGAAGAAGGGAAGTGTAAATTTTCTTGCACTGATTCAGATGGATATATGAAGATAGAAGTGTCCTGCTTCTACTTGTGTATTTTTTTTTTTCAATTCATTTGCATGATTTGTCTTCTGTCTGGCTATAAAGGTCATTTGAAACTTCATATAGGGTCATGCGATGGGCCTAGACAAATTTGAGGTTAACTTTTCTTGCTTTCTTTTGAAGAAAACTAGNGGTTCTGTCTTTTTGTAGTTGGTTTCTATTTCA
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00471 Ps_contig_mira-and-tgicl-ass_franssen_4963
SEQUENCE=CGAGAGCAAACATCATTTTATTTGKTCTTTGATGAGATCCAACTTTTCATTATTGTATGTTTAAGTTCACACACAATAAAGGTGCAATGCAAAGGCTTGTATATGTGTGTAAATTAAAACTCATACAGGGATAAATACACTGGGGAGCGACTGTATTCAGTATGAAATTAACCCATATCCCGGGATGGGAAACGGTGGGATCTAAGTCAAAAGTGGAAGAACAATATATAATACCACTTCCCATATATTTCTAACTATTTGTTACATTTTCAATTGAAGGAGAAACAACGCTTGTTGTCATATCCAATTTGAGCGCATGCCTTGTAATCTGGTTTGTTTGTTTTCTCAATCTAACATTATCAACGAACAAATCTGCTATGATCTTTCTCATTTCATCACCACAGATTGCACCATCTTGTATGACATTGGTTTTTTCCCCGATAAAAGCTGCATTTCCGTAGTCCTTCTCTAAGTTTTCTACCTCTGCTAGTAGGATGTTGATTTGGTCATCAGATGTCTTTAATAGATTAAAAGCATCAGTTGATGTTGATGAATGCAAGACTGTCCTATTCTCGTCATTGAAAGGAAGATCGACATTGCATTCTTGAAGCTGACTTAAAAAGAAGGCCACATTTCTCAAGCAATTCTCTAGAAGCTGTTTCTGCTTGTTTCCCTTTTCTCTCTTTCATGAAGTTAGTTAGTTTTCCTCCGCTTTCTTAACG
TARGET=332,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00472 Ps_contig_mira-and-tgicl-ass_franssen_4963
SEQUENCE=CGAGAGCAAACATCATTTTATTTGKTCTTTGATGAGATCCAACTTTTCATTATTGTATGTTTAAGTTCACACACAATAAAGGTGCAATGCAAAGGCTTGTATATGTGTGTAAATTAAAACTCATACAGGGATAAATACACTGGGGAGCGACTGTATTCAGTATGAAATTAACCCATATCCCGGGATGGGAAACGGTGGGATCTAAGTCAAAAGTGGAAGAACAATATATAATACCACTTCCCATATATTTCTAACTATTTGTTACATTTTCAATTGAAGGAGAAACAACGCTTGTTGTCATATCCAATTTGAGCGCATGCCTTGTAATCTGGTTTGTTTGTTTTCTCAATCTAACATTATCAACGAACAAATCTGCTATGATCTTTCTCATTTCATCACCACAGATTGCACCATCTTGTATGACATTGGTTTTTTCCCCGATAAAAGCTGCATTTCCGTAGTCCTTCTCTAAGTTTTCTACCTCTGCTAGTAGGATGTTGATTTGGTCATCAGATGTCTTTAATAGATTAAAAGCATCAGTTGATGTTGATGAATGCAAGACTGTCCTATTCTCGTCATTGAAAGGAAGATCGACATTGCATTCTTGAAGCTGACTTAAAAAGAAGGCCACATTTCTCAAGCAATTCTCTAGAAGCTGTTTCTGCTTGTTTCCCTTTTCTCTCTTTCATGAAGTTAGTTAGTTTTCCTCCGCTTTCTTAACG
TARGET=692,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00473 Ps_contig_mira-and-tgicl-ass_franssen_4987
SEQUENCE=AAGAAAGAACTCAAGGGATGAGGATGATTATAAGGAGAGAAGGAGCTCTAGGGATGAGGATGTGCGTGAGGAAAGAAAGCACAGAGGGGGTGAGGATTATCATGGAGAAAGAAAGCGCAGAAGGGATGATGATGATCATGTGCATGGAAGAAAGAGCATTANAGAAGGNTGATGATGATGGAAGAAAAGAAAGTACAGGAAGGGAGTTAGTTT
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1829
PRIMER_LEFT_PENALTY=0.109273
PRIMER_RIGHT_PENALTY=0.073614
PRIMER_LEFT_SEQUENCE=AGGAGAGAAGGAGCTCTAGGGA
PRIMER_RIGHT_SEQUENCE=TCTTCCATCATCATCANCCTTC
PRIMER_LEFT=32,22
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.75
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.4889
PRIMER_LEFT_1_PENALTY=0.415247
PRIMER_RIGHT_1_PENALTY=0.073614
PRIMER_LEFT_1_SEQUENCE=GTGAGGAAAGAAAGCACAGAGG
PRIMER_RIGHT_1_SEQUENCE=TCTTCCATCATCATCANCCTTC
PRIMER_LEFT_1=65,22
PRIMER_RIGHT_1=184,22
PRIMER_LEFT_1_TM=60.415
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=0.7085
PRIMER_LEFT_2_PENALTY=0.073866
PRIMER_RIGHT_2_PENALTY=0.634599
PRIMER_LEFT_2_SEQUENCE=AGAACTCAAGGGATGAGGATGA
PRIMER_RIGHT_2_SEQUENCE=TAATGCTCTTTCTTCCATGCAC
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=160,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.365
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.7439
PRIMER_LEFT_3_PENALTY=0.109273
PRIMER_RIGHT_3_PENALTY=0.634599
PRIMER_LEFT_3_SEQUENCE=AGGAGAGAAGGAGCTCTAGGGA
PRIMER_RIGHT_3_SEQUENCE=TAATGCTCTTTCTTCCATGCAC
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=160,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.365
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.8678
PRIMER_LEFT_4_PENALTY=0.233224
PRIMER_RIGHT_4_PENALTY=0.634599
PRIMER_LEFT_4_SEQUENCE=GAGCTCTAGGGATGAGGATGTG
PRIMER_RIGHT_4_SEQUENCE=TAATGCTCTTTCTTCCATGCAC
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=160,22
PRIMER_LEFT_4_TM=60.233
PRIMER_RIGHT_4_TM=59.365
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_00474 Ps_contig_mira-and-tgicl-ass_franssen_4988
SEQUENCE=TCTTTCTTTCTCCAATGCCACTCTCATTCCCTTTTGTTTTGTGCTTCTCTATGACGATCATCATCCCTTAATTTCTTTCTTTCTTCATAATCATCCCCGTCCCTCAAGGTCTTTCTTCCTGTACGTTCATCCTCAGTCCCTCTAATCTTTGTTTCTATGTGACCACCCTCATCCCTCAAGTGCTTTCTTCCTATAGGAAAATCTTCATCCTTTCCATGTTTTCTCTCTCCACGACCATCTTCATCACCTCTTAAGCTCTTTTTCTCTTGGTGAACTTCCCTGCCTCTTTTATGTTTTCCATCTCTTGAATCATCCTCATCCCTTAAGTGCTTTCTTTCTATGCGCTCGTCTTCATTCCTTCCATGTTTTTTTTCTCCATGGTTATCCTGATCCTGTTTATAATCATTATCATGTCTTCCCTTCCTTCTTGTATCATAATCCTCCCTATACCGATCAATTCGTCCAACAGATGGTGATTCAACATCCTTGTTATAATTTCTGCCTCTCTGCTCAGAAGTCTCAATATCATTGCCTCTGAGCCTAGCTGACTTAGAATAACCCTCTTTTTCTGGATTTTCATTTTGATAAATTCGAGATTTTGACTCAGACTTCCTACCCTCTCCATCATGCTTAGCCAATTTGAAAGTCTCCCTATCTCTATCTTCATCAATCTTCTTGTCACCCTTTGCCATTGCTTGACTTCTAGAATTTGGATCCTCTTTAAAATCTCTCCCGTATGATTTTCTTAAAGTATCCAATCCATCAGTATTAGCACTACTTTTCSCBTTTTCGGCAGCAATACTCTGTACTATTTATTTCTCCTTCG
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00475 Ps_contig_mira-and-tgicl-ass_franssen_4989
SEQUENCE=AAAGAAAGAACTCAAGGGATGAGGATGATTATAAGGAGAGAAGGAGCTCTAGGGATGAGGATGTGCGTGAGGAAAGAAGCACAGAGGGGGTGAGGATTATCATGGAAGAAAGAAGCGCAAGGAAGGGATGATGATGATCATGTGCAGTGGAAGAAAGGAGCTATTAGAGGAAGGNTGATGATGATGGAAGAAAAGTAAACGTACGAAGGG
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1829
PRIMER_LEFT_PENALTY=0.109273
PRIMER_RIGHT_PENALTY=0.073614
PRIMER_LEFT_SEQUENCE=AGGAGAGAAGGAGCTCTAGGGA
PRIMER_RIGHT_SEQUENCE=TCTTCCATCATCATCANCCTTC
PRIMER_LEFT=33,22
PRIMER_RIGHT=190,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.75
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=0.9965
PRIMER_LEFT_1_PENALTY=0.073866
PRIMER_RIGHT_1_PENALTY=0.922591
PRIMER_LEFT_1_SEQUENCE=AGAACTCAAGGGATGAGGATGA
PRIMER_RIGHT_1_SEQUENCE=TAGCTCCTTTCTTCCACTGCAC
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=162,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.923
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=1.0246
PRIMER_LEFT_2_PENALTY=0.073866
PRIMER_RIGHT_2_PENALTY=0.950696
PRIMER_LEFT_2_SEQUENCE=AGAACTCAAGGGATGAGGATGA
PRIMER_RIGHT_2_SEQUENCE=AATAGCTCCTTTCTTCCACTGC
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=164,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.049
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=1.1558
PRIMER_LEFT_3_PENALTY=0.233224
PRIMER_RIGHT_3_PENALTY=0.922591
PRIMER_LEFT_3_SEQUENCE=GAGCTCTAGGGATGAGGATGTG
PRIMER_RIGHT_3_SEQUENCE=TAGCTCCTTTCTTCCACTGCAC
PRIMER_LEFT_3=43,22
PRIMER_RIGHT_3=162,22
PRIMER_LEFT_3_TM=60.233
PRIMER_RIGHT_3_TM=60.923
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=1.1839
PRIMER_LEFT_4_PENALTY=0.233224
PRIMER_RIGHT_4_PENALTY=0.950696
PRIMER_LEFT_4_SEQUENCE=GAGCTCTAGGGATGAGGATGTG
PRIMER_RIGHT_4_SEQUENCE=AATAGCTCCTTTCTTCCACTGC
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=164,22
PRIMER_LEFT_4_TM=60.233
PRIMER_RIGHT_4_TM=59.049
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=122
=
PRIMER_SEQUENCE_ID=SSR_00476 Ps_contig_mira-and-tgicl-ass_franssen_4993
SEQUENCE=TGTGTTTGAGTTGCAAGTAAAACTTCTCAAGCAAGTTCCAGAAGAGTATCCATATAAGGATGATGAGTAGTAATGTTCTGTTTTGTATTGTTGTACTTCATCATCCCAAGTATTAGATTATTATTATTATTATCTTTCTTGCCTTGTRTGCATTTTGACAAAACTAAAAATAACGATAGAAAATATTTAATTTAAATCATTATGATAATTGGGATTTAARACTTAAAAAA
TARGET=117,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00477 Ps_contig_mira-and-tgicl-ass_franssen_4994
SEQUENCE=AATTCTTAACTTATAAAYCCCTAATTATCATAATGTATTTAATTAAATTATTTTCTATCGTTATTTTTTAGTTTTGTACAAAATGCACACAAGGCAGAAAGATAATAATAATAATCTAATACTTGGGATGATGAAGTACAACAATAACAAAACAGAACATTACTACTCATCATCCTTATATGGATACTCTTCTGGAACTTGCTTGAGTAAGTTTTCTGCAACTACAAACAC
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00478 Ps_contig_mira-and-tgicl-ass_franssen_4997
SEQUENCE=CAACCCTCTGAACCATTGTAAATTGAAATATTCTCCTCTTCCTCAGGTCGACATCCACGAACCCAAATCCGTTGTTTATACTTGTTCTCCTCCACAAATAAACTACTCATGATCTTTCTTTCTTTGTGACCCAATAAAACTCTGTAAGTAGAATGATGAAAAATAACGCCTGAACCTTTCAAACTGCACAACAAGAGTTTAGCTATTTGCTTTACCATCAACATATACAATACACTATATAGTTGCAATCAAATTAAAAAGATGTGTTATTGCTTAATAAAAAAAGACGTTGTGTTATTTTTGGCACAAAATAGGAACAAA
TARGET=113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3084
PRIMER_LEFT_PENALTY=0.157667
PRIMER_RIGHT_PENALTY=0.150698
PRIMER_LEFT_SEQUENCE=TATTCTCCTCTTCCTCAGGTCG
PRIMER_RIGHT_SEQUENCE=GGTTCAGGCGTTATTTTTCATC
PRIMER_LEFT=28,22
PRIMER_RIGHT=175,22
PRIMER_LEFT_TM=59.842
PRIMER_RIGHT_TM=59.849
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=0.3199
PRIMER_LEFT_1_PENALTY=0.157667
PRIMER_RIGHT_1_PENALTY=0.162217
PRIMER_LEFT_1_SEQUENCE=TATTCTCCTCTTCCTCAGGTCG
PRIMER_RIGHT_1_SEQUENCE=AGTTTGAAAGGTTCAGGCGTTA
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=184,22
PRIMER_LEFT_1_TM=59.842
PRIMER_RIGHT_1_TM=60.162
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=0.3473
PRIMER_LEFT_2_PENALTY=0.157667
PRIMER_RIGHT_2_PENALTY=0.189596
PRIMER_LEFT_2_SEQUENCE=TATTCTCCTCTTCCTCAGGTCG
PRIMER_RIGHT_2_SEQUENCE=TGTTGTGCAGTTTGAAAGGTTC
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=192,22
PRIMER_LEFT_2_TM=59.842
PRIMER_RIGHT_2_TM=60.190
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.4755
PRIMER_LEFT_3_PENALTY=0.157667
PRIMER_RIGHT_3_PENALTY=0.317850
PRIMER_LEFT_3_SEQUENCE=TATTCTCCTCTTCCTCAGGTCG
PRIMER_RIGHT_3_SEQUENCE=TCTTGTTGTGCAGTTTGAAAGG
PRIMER_LEFT_3=28,22
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=59.842
PRIMER_RIGHT_3_TM=60.318
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=0.4755
PRIMER_LEFT_4_PENALTY=0.157667
PRIMER_RIGHT_4_PENALTY=0.317850
PRIMER_LEFT_4_SEQUENCE=TATTCTCCTCTTCCTCAGGTCG
PRIMER_RIGHT_4_SEQUENCE=TTGTGCAGTTTGAAAGGTTCAG
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=190,22
PRIMER_LEFT_4_TM=59.842
PRIMER_RIGHT_4_TM=60.318
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_00479 Ps_contig_mira-and-tgicl-ass_franssen_5015
SEQUENCE=CCATCTGGAGRWTTGTTTTTGTAGTACTAAGGGCTCGATGGAGCCTTCAGTCAGCCCTGGTAGCTCTGAGAGGCTGCCAGAGTAAAAATCTGACATTGGGCTTTTTTCTTTCAGTGCTGGTGACGGAGAAAAAGGTTCGCCAAATATTTGAGGTAATGCTGTTGGAGTTGGGGCATTTCCACCTTCCTCGATAGCTGAGATAGGTATAGAGTTCATGGTGCAGTGCACTCTTCTTGGCCCTCTAGTCCAGAGAGCATTCAGCTCATAGGAAACAGTGCTAACAAGATTGCTACATGCTGGAACTCTTGGTGACACCTGCTTAGAATTAAATATCCCACTTGGACGATGCTTTGGTTGAACTGCAGCATCATGCGGGGGTTGACTGTCGTATACAGTGAATTTGGTGCCCCAAAAATTAGACCTCAGTTTACCAACATATTTGTTGCTGGCTCGAGAAAAATTGTCTGCAGCCAAGGATATGATAAAGCCGGTACCTACGGCCCTCCTTATCTTTCTGGCTGCTAGTAACAACTTATTACTCTCATGCTCCGATGGCACCAAACCTAAGTACAACACGAACGTAGAAGTTTCTCTGTTCCTCCTAATAAAGCACTGTATTGGATAATCACGGGGACCGGGCTGCTTCAATGAAATAGGAAATGTGATCCTTCCACATTGCTGAGGAGTCTTGATGATCTCTTTCATAACAGACCTCCATGATTTACATACCGAAGCACAAAAGACAACAACAGCACGTGCAGGCCAAGATGTCTCACTCTCTTCGACCCTCCGGATTATATCCAAAAGCAATTCAGGTGGTAGACTTGCCCACTGTCCCTGTTGAATAGGTTCAGTCGGAGTTACATCCGGAGCAACATGTGACTTCGACCGAGAGAGCCAATGCTTGCTCTCACCACCCCGCCTTGATATGCTACCTATTCCATTTTTCATCCCCTTGAGTTCACGGACTATACTTTTCAGCGACATGATTCCACGCCTTACAAACTATGTCAATCCAACCACAAGAAGCTTCTAGATTTCTGATGCTAACTGAATGAAARAAAAAAAGAGATAAATAGCAACAAGTTTAGAATACCGATCTACTAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAACGTTATTTGAATTTGTGGCGAAAATTAGTTGACATCGCTCAGTTAAGCGCCACCGCGATTCTGCGGAATCGCGTTTGTGGAATTAGATCAAGCTTTTTTTATCATGTCTCATTTTTATAATAATTGATTATTTCSSTC
TARGET=1106,45
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00480 Ps_contig_mira-and-tgicl-ass_franssen_5018
SEQUENCE=TCGTAATATGACATTCTCAACATGTAATCAACAAGAATTCGCTTCAAGCGAGTGTTATTCCATTCCGACATTTTTCTGCATCTGCAGATTCCAAATGTTCGAGACGTGCACGACACTTCTGCGCTTGCAGATGCTCAGCTCGATTTCCTTCTTCCAATTTTCTTTTGAGTCCTTGGAGGCGGGAGACGAGAGAGCTAAGGTGATTAACGGCGTCGGAGGGGGATAAATCAGAAGAGACGGCATCGTTGACGCCGGAGATAACGGCGGTCATCTCCTTCTCGACGACACGGTTGTTGGCTCGGAGGGTTTTCTTGAAGTGCTCGAAAGGGACTCTGAGGAACTGATGTTCGAGCTTGAGAGACTCCGTCAGTCGGGGAAGGTTGGAGGACGGAGCAGGCGTAGGGTTGACGGCGGCGGTGGCTCCGATTTGAGTTCCGGTGGCAGTGTTGTTTCCGTTCGGAGTGGAATCCATTTCCATTGGGGAAGGAAGGAACGAAAAATTGGAATCTCACAGATTGCAACTTGTAACTGGATAAATGCTCTTACAAAACTAAATCC
TARGET=482,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.4339
PRIMER_LEFT_PENALTY=0.023267
PRIMER_RIGHT_PENALTY=3.410595
PRIMER_LEFT_SEQUENCE=ATGTTCGAGCTTGAGAGACTCC
PRIMER_RIGHT_SEQUENCE=GAGCATTTATCCAGTTACAAGTTGC
PRIMER_LEFT=343,22
PRIMER_RIGHT=541,25
PRIMER_LEFT_TM=60.023
PRIMER_RIGHT_TM=60.411
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=3.5546
PRIMER_LEFT_1_PENALTY=0.143960
PRIMER_RIGHT_1_PENALTY=3.410595
PRIMER_LEFT_1_SEQUENCE=GAAAGGGACTCTGAGGAACTGA
PRIMER_RIGHT_1_SEQUENCE=GAGCATTTATCCAGTTACAAGTTGC
PRIMER_LEFT_1=322,22
PRIMER_RIGHT_1=541,25
PRIMER_LEFT_1_TM=59.856
PRIMER_RIGHT_1_TM=60.411
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=220
PRIMER_PAIR_PENALTY_2=3.6101
PRIMER_LEFT_2_PENALTY=0.199477
PRIMER_RIGHT_2_PENALTY=3.410595
PRIMER_LEFT_2_SEQUENCE=GAGCTTGAGAGACTCCGTCAGT
PRIMER_RIGHT_2_SEQUENCE=GAGCATTTATCCAGTTACAAGTTGC
PRIMER_LEFT_2=349,22
PRIMER_RIGHT_2=541,25
PRIMER_LEFT_2_TM=60.199
PRIMER_RIGHT_2_TM=60.411
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=193
PRIMER_PAIR_PENALTY_3=3.6713
PRIMER_LEFT_3_PENALTY=0.260719
PRIMER_RIGHT_3_PENALTY=3.410595
PRIMER_LEFT_3_SEQUENCE=AAGGGACTCTGAGGAACTGATG
PRIMER_RIGHT_3_SEQUENCE=GAGCATTTATCCAGTTACAAGTTGC
PRIMER_LEFT_3=324,22
PRIMER_RIGHT_3=541,25
PRIMER_LEFT_3_TM=59.739
PRIMER_RIGHT_3_TM=60.411
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=3.8061
PRIMER_LEFT_4_PENALTY=0.395499
PRIMER_RIGHT_4_PENALTY=3.410595
PRIMER_LEFT_4_SEQUENCE=GGGACTCTGAGGAACTGATGTT
PRIMER_RIGHT_4_SEQUENCE=GAGCATTTATCCAGTTACAAGTTGC
PRIMER_LEFT_4=326,22
PRIMER_RIGHT_4=541,25
PRIMER_LEFT_4_TM=59.605
PRIMER_RIGHT_4_TM=60.411
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_00481 Ps_contig_mira-and-tgicl-ass_franssen_5023
SEQUENCE=GAGAGCAGTGGTTTTTACGACGGCGAAATTGAGGTCGATGTTGTTGTTGTTGAGGATAGGGAAGACGGTGATGATGATGAAGGAAATCAGTAGATAATGATGAATTGAGTGGTTGTGGAAGAAAGCGGAGAAACGCCATGTTCACGGTGGTGATGCTGCAGATCTATACTCGGAGAAGATGATTGTTTGTTGTATTYGTTACTACCTCCCCGCGTACTCTGCGTT
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00482 Ps_contig_mira-and-tgicl-ass_franssen_5023
SEQUENCE=GAGAGCAGTGGTTTTTACGACGGCGAAATTGAGGTCGATGTTGTTGTTGTTGAGGATAGGGAAGACGGTGATGATGATGAAGGAAATCAGTAGATAATGATGAATTGAGTGGTTGTGGAAGAAAGCGGAGAAACGCCATGTTCACGGTGGTGATGCTGCAGATCTATACTCGGAGAAGATGATTGTTTGTTGTATTYGTTACTACCTCCCCGCGTACTCTGCGTT
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00483 Ps_contig_mira-and-tgicl-ass_franssen_5026
SEQUENCE=ATTCCATGTCATGTCATGTGGTTCTTTAAAAATTTTAACAACTCCATGCCATGCCATGCCATTCTCAGCTATCTTTTTCATGTACTTTTGTGTTTTCTGTAAATATGATATGTTGTTAAAGTGCTGATAAATAGATAATAAGCACTTTGATAGTTACTTAATATAGTATATAATATATTGTGGTGGGAACATGAGAATTTGTTATT
TARGET=5,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00484 Ps_contig_mira-and-tgicl-ass_franssen_5026
SEQUENCE=ATTCCATGTCATGTCATGTGGTTCTTTAAAAATTTTAACAACTCCATGCCATGCCATGCCATTCTCAGCTATCTTTTTCATGTACTTTTGTGTTTTCTGTAAATATGATATGTTGTTAAAGTGCTGATAAATAGATAATAAGCACTTTGATAGTTACTTAATATAGTATATAATATATTGTGGTGGGAACATGAGAATTTGTTATT
TARGET=44,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00485 Ps_contig_mira-and-tgicl-ass_franssen_5027
SEQUENCE=ATTCCATGTCATGTCATGTGGTTCTTAAAAATTTAACTCCATGCCATGCCATGCCATTCTCAGCTATCTTTTTCATGTACTTTTGTGTTTTCTGTAAAATATGATATGTTGTTAAAGTGCTGATAAATAGATAATAAGCACTTTGATAGTTACTTAATATAGTATATAATATATTGCGGTTGGGAAACATGAGAATTTGTATCAAATTCAGAAGAAGGATGTGTTGGAATTATTGAAGTTACTATGAAAAGACAAATTAATCTGTAC
TARGET=5,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00486 Ps_contig_mira-and-tgicl-ass_franssen_5027
SEQUENCE=ATTCCATGTCATGTCATGTGGTTCTTAAAAATTTAACTCCATGCCATGCCATGCCATTCTCAGCTATCTTTTTCATGTACTTTTGTGTTTTCTGTAAAATATGATATGTTGTTAAAGTGCTGATAAATAGATAATAAGCACTTTGATAGTTACTTAATATAGTATATAATATATTGCGGTTGGGAAACATGAGAATTTGTATCAAATTCAGAAGAAGGATGTGTTGGAATTATTGAAGTTACTATGAAAAGACAAATTAATCTGTAC
TARGET=39,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00487 Ps_contig_mira-and-tgicl-ass_franssen_5028
SEQUENCE=TCGGGGATACTGCCATGGCCACACGTTCATAAGAAAAGAAAAAGNGAGAGTGAAACTTGTAATAGAGTAAGGAAAGCTTTTGCGAAAGAGTCAGAATAATCAACATTCATCATCATCATCATCATCATCTAGAATGGCCACTATATCATCATCTTTGCCAACAAATAGTAAACCTNTTTTCTCAGTATCNGCAAGACCACTACTTTCTTTGTTCCTTT
TARGET=107,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2208
PRIMER_LEFT_PENALTY=0.027715
PRIMER_RIGHT_PENALTY=0.193133
PRIMER_LEFT_SEQUENCE=GCTTTTGCGAAAGAGTCAGAAT
PRIMER_RIGHT_SEQUENCE=TGGTCTTGCNGATACTGAGAAA
PRIMER_LEFT=75,22
PRIMER_RIGHT=198,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=59.807
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=0.3752
PRIMER_LEFT_1_PENALTY=0.182061
PRIMER_RIGHT_1_PENALTY=0.193133
PRIMER_LEFT_1_SEQUENCE=AAAGCTTTTGCGAAAGAGTCAG
PRIMER_RIGHT_1_SEQUENCE=TGGTCTTGCNGATACTGAGAAA
PRIMER_LEFT_1=72,22
PRIMER_RIGHT_1=198,22
PRIMER_LEFT_1_TM=60.182
PRIMER_RIGHT_1_TM=59.807
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=0.5057
PRIMER_LEFT_2_PENALTY=0.312541
PRIMER_RIGHT_2_PENALTY=0.193133
PRIMER_LEFT_2_SEQUENCE=AGAGTAAGGAAAGCTTTTGCGA
PRIMER_RIGHT_2_SEQUENCE=TGGTCTTGCNGATACTGAGAAA
PRIMER_LEFT_2=63,22
PRIMER_RIGHT_2=198,22
PRIMER_LEFT_2_TM=59.687
PRIMER_RIGHT_2_TM=59.807
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.7310
PRIMER_LEFT_3_PENALTY=0.027715
PRIMER_RIGHT_3_PENALTY=0.703266
PRIMER_LEFT_3_SEQUENCE=GCTTTTGCGAAAGAGTCAGAAT
PRIMER_RIGHT_3_SEQUENCE=GTGGTCTTGCNGATACTGAGAA
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=199,22
PRIMER_LEFT_3_TM=60.028
PRIMER_RIGHT_3_TM=59.297
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=0.8543
PRIMER_LEFT_4_PENALTY=0.661199
PRIMER_RIGHT_4_PENALTY=0.193133
PRIMER_LEFT_4_SEQUENCE=AGCTTTTGCGAAAGAGTCAGAA
PRIMER_RIGHT_4_SEQUENCE=TGGTCTTGCNGATACTGAGAAA
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=198,22
PRIMER_LEFT_4_TM=60.661
PRIMER_RIGHT_4_TM=59.807
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_00488 Ps_contig_mira-and-tgicl-ass_franssen_5033
SEQUENCE=TACCACGAAACGGAAGTACAACGAAACCGAAAAAAGGAAAAAGGAAATAGAAGAAAGAAAGAAACAAGTGAAACTAGATCAGTTCCGAGGGTTTCGGTAAWKTWCGTTGGTTTGGTACAAAGAAAGACTGCCATGGTTTCTTCAGACCATTCGCAACATGCTTACTTCTCCAGGGAGTTTCGCTGAAAGCCAACACGGAGAAGTGACTTTTCCTGAGATCAGTACCACTATTCTTGAGAAGATTTGCCAGTATTTTTATTGGCATCTTGAATTCGCCAGTGGAAAGGACAGAGAGTTTCCTATTGAACCGGAATTAACTCTGGAGTTAATGATGGCTGCCAATTACCTACATACTTGAGCATATTCACTTCCCAAAGGTAATGCAGAAGAAACTGTTGCTTTGCTGGGCTATGTAAAAATACAAGCTACTATTGTTGTTGTCGTTATAAACTAGAATATACATGTAAGGGGAGATGTCAACTCCTCGTCTTACTTCTTTGTAGTCTTTTAATTTTGGGCTTTGACACTTAATATTTGTTAAAAATAATATGATAGCTAGCTAGATTCAGCTGTTAGACTGACCTGATTGATAATATGATTAGTTAGATTCAGACTWAAGTT
TARGET=51,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00489 Ps_contig_mira-and-tgicl-ass_franssen_5037
SEQUENCE=ACGTCGGGGATGGGTAGAAAAGAAGAAGAAGAAGTGGCGGTGGGAGTACCAGTTTATGAAAGAAACGGCATCCCACCAAACGCGTTGATCGGAGATCCCAAAGGTATTCCCATCCAACAGACTATTTACAGAGACACTCCTGCTCCCTTCAATTGTCCTCACTGCGGTCACACCTCTCTCACCTCTATCCAAGATTAGTCTGGCTGCATTCGTTAGGATGCTTGATGCCGA
TARGET=20,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00490 Ps_contig_mira-and-tgicl-ass_franssen_5052
SEQUENCE=GCAGTGGTTATTCAACGCAGAGTTATTTATATTAAAGTTTTTTTTTTAATTTAAAATCTTTCCATTTTTCCTACGGCGGCGGCGGCTACCAAACAGGACACGACATAACACAGCCCTCCATCAAGTGAATCGAAGGAAATTTCTGACCATGGCTTCAACCATTCCTGTTGAACAAGTCAACAGTGGTCCGCTTCAGGTTACTGGTGACTCCTTTATCAGACAACATCTTAGGAAATTGGCTCCTTATCAACCCATTTTGCCTTTTGAGGTTTTATCTTCTCGTCTTGGACGGAAGCCGGAGGATATTGTCAAGTTAGATGCTAATGAGAATCCTTACGGCCCTCCTCCAGAGGTAATGGAAGCCCTAGGGTCAATACGATTCCCATATGTCTATCCAGATCCAGAGAGCCGTCGGTTGCGTGAAGCTCTCGCTCAAGATTCAGGCCTTGAATCTGAGTATATTCTTGTAGGATGTGGTGCCGACGAGCTTATTGATTTGATCATGCGTTGTGTGCTTGATCCTGGTGACAAGATCGTCGACTGCCCTCCAACTTTCACAATGTATGAATTTGATGCCGCAGTTAACGGAGCACTCGTCATCAAAGTTCCAAGGAAGCCAGACTTCAGCTTGAATGTCGAACACATAATCGAGGTTGTTGAACAAGAGAAGCCCAAATGCATATTTTTAACATCTCCTAACAATCCAGATGGGAGTGTAATTAATGACGATGATCTCTTAAAGATACTCGAGCTTCCCATACTGGTGGTGCTGGATGAAGCATACATCGAGTTTTCACAAATTGAATCAAAGATGAGTTGGGTGAAGAAACATGAGAATCTCATTATTCTTCGAACATTCAGCAAAAGAGCTGGTTTAGCTGGACTTCGTGTGGGATATGGAGCTTTTCCTTTGAGTTTAATTAAGTATCTTTGGAGAGCAAAGCAACCTTATAATGTATCCGTTGCAGCCGAAGTTTCTGCATGCGCAGCACTGGAAAATCCTACCTATCTAGAGAATGTGAAAGATGCTTTGGTGAAAGAAAGAGGGAGGCTTTTTGAGCTTTTGAAGGCAGTTCCATTCCTTAGGCCATTTCCAAGCCATTCTAATTTCATTCTTTGTGAGGTTACATCTGGATTTGATGCAAAGAAGTTAAAGGATGATCTTGCAGCAATGGGCGTGATGATTCGTCATTACAGCAGTAAAGAGCTGAAAGGCTACGTTCGAGTATCGGTTGGGAAGCCTGAACACACGGACGCGCTTATGAACGGCCTCAACAGACTATTGTAGGAGAAAAATTTCACTGTGATCGATCGATCATACAAAGAGATTCTCTTACATAACATTCAAAACTAGAGGAAAATGTATTATGAGAAGTTGAGCTAGTTTATGTTATGCAGACAATAATGAGTTCATCTCAGATATACAACACCAACGAACGTAATCTTAAGTTGTAACATTTGTATTATGTATTTTAAGGTGGTTGATTCAGGACATAAGTTACTTCCTTGTTTAATAAATTATCTTTAAGGTGAGAAATCAAAATC
TARGET=74,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6993
PRIMER_LEFT_PENALTY=0.691033
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=CAGTGGTTATTCAACGCAGAGT
PRIMER_RIGHT_SEQUENCE=TTGACTTGTTCAACAGGAATGG
PRIMER_LEFT=1,22
PRIMER_RIGHT=179,22
PRIMER_LEFT_TM=59.309
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.7944
PRIMER_LEFT_1_PENALTY=0.691033
PRIMER_RIGHT_1_PENALTY=0.103353
PRIMER_LEFT_1_SEQUENCE=CAGTGGTTATTCAACGCAGAGT
PRIMER_RIGHT_1_SEQUENCE=GCTGTGTTATGTCGTGTCCTGT
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=113,22
PRIMER_LEFT_1_TM=59.309
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=0.7992
PRIMER_LEFT_2_PENALTY=0.691033
PRIMER_RIGHT_2_PENALTY=0.108206
PRIMER_LEFT_2_SEQUENCE=CAGTGGTTATTCAACGCAGAGT
PRIMER_RIGHT_2_SEQUENCE=ACTTGATGGAGGGCTGTGTTAT
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=125,22
PRIMER_LEFT_2_TM=59.309
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.8656
PRIMER_LEFT_3_PENALTY=0.691033
PRIMER_RIGHT_3_PENALTY=0.174518
PRIMER_LEFT_3_SEQUENCE=CAGTGGTTATTCAACGCAGAGT
PRIMER_RIGHT_3_SEQUENCE=GGAGTCACCAGTAACCTGAAGC
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=210,22
PRIMER_LEFT_3_TM=59.309
PRIMER_RIGHT_3_TM=60.175
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=1.1947
PRIMER_LEFT_4_PENALTY=0.691033
PRIMER_RIGHT_4_PENALTY=0.503696
PRIMER_LEFT_4_SEQUENCE=CAGTGGTTATTCAACGCAGAGT
PRIMER_RIGHT_4_SEQUENCE=TGACTTGTTCAACAGGAATGGT
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=59.309
PRIMER_RIGHT_4_TM=59.496
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_00491 Ps_contig_mira-and-tgicl-ass_franssen_5052
SEQUENCE=GCAGTGGTTATTCAACGCAGAGTTATTTATATTAAAGTTTTTTTTTTAATTTAAAATCTTTCCATTTTTCCTACGGCGGCGGCGGCTACCAAACAGGACACGACATAACACAGCCCTCCATCAAGTGAATCGAAGGAAATTTCTGACCATGGCTTCAACCATTCCTGTTGAACAAGTCAACAGTGGTCCGCTTCAGGTTACTGGTGACTCCTTTATCAGACAACATCTTAGGAAATTGGCTCCTTATCAACCCATTTTGCCTTTTGAGGTTTTATCTTCTCGTCTTGGACGGAAGCCGGAGGATATTGTCAAGTTAGATGCTAATGAGAATCCTTACGGCCCTCCTCCAGAGGTAATGGAAGCCCTAGGGTCAATACGATTCCCATATGTCTATCCAGATCCAGAGAGCCGTCGGTTGCGTGAAGCTCTCGCTCAAGATTCAGGCCTTGAATCTGAGTATATTCTTGTAGGATGTGGTGCCGACGAGCTTATTGATTTGATCATGCGTTGTGTGCTTGATCCTGGTGACAAGATCGTCGACTGCCCTCCAACTTTCACAATGTATGAATTTGATGCCGCAGTTAACGGAGCACTCGTCATCAAAGTTCCAAGGAAGCCAGACTTCAGCTTGAATGTCGAACACATAATCGAGGTTGTTGAACAAGAGAAGCCCAAATGCATATTTTTAACATCTCCTAACAATCCAGATGGGAGTGTAATTAATGACGATGATCTCTTAAAGATACTCGAGCTTCCCATACTGGTGGTGCTGGATGAAGCATACATCGAGTTTTCACAAATTGAATCAAAGATGAGTTGGGTGAAGAAACATGAGAATCTCATTATTCTTCGAACATTCAGCAAAAGAGCTGGTTTAGCTGGACTTCGTGTGGGATATGGAGCTTTTCCTTTGAGTTTAATTAAGTATCTTTGGAGAGCAAAGCAACCTTATAATGTATCCGTTGCAGCCGAAGTTTCTGCATGCGCAGCACTGGAAAATCCTACCTATCTAGAGAATGTGAAAGATGCTTTGGTGAAAGAAAGAGGGAGGCTTTTTGAGCTTTTGAAGGCAGTTCCATTCCTTAGGCCATTTCCAAGCCATTCTAATTTCATTCTTTGTGAGGTTACATCTGGATTTGATGCAAAGAAGTTAAAGGATGATCTTGCAGCAATGGGCGTGATGATTCGTCATTACAGCAGTAAAGAGCTGAAAGGCTACGTTCGAGTATCGGTTGGGAAGCCTGAACACACGGACGCGCTTATGAACGGCCTCAACAGACTATTGTAGGAGAAAAATTTCACTGTGATCGATCGATCATACAAAGAGATTCTCTTACATAACATTCAAAACTAGAGGAAAATGTATTATGAGAAGTTGAGCTAGTTTATGTTATGCAGACAATAATGAGTTCATCTCAGATATACAACACCAACGAACGTAATCTTAAGTTGTAACATTTGTATTATGTATTTTAAGGTGGTTGATTCAGGACATAAGTTACTTCCTTGTTTAATAAATTATCTTTAAGGTGAGAAATCAAAATC
TARGET=1306,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9613
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.935692
PRIMER_LEFT_SEQUENCE=ATGAACGGCCTCAACAGACTAT
PRIMER_RIGHT_SEQUENCE=AAGATTACGTTCGTTGGTGTTG
PRIMER_LEFT=1261,22
PRIMER_RIGHT=1445,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=59.064
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=1.1670
PRIMER_LEFT_1_PENALTY=0.231323
PRIMER_RIGHT_1_PENALTY=0.935692
PRIMER_LEFT_1_SEQUENCE=TATGAACGGCCTCAACAGACTA
PRIMER_RIGHT_1_SEQUENCE=AAGATTACGTTCGTTGGTGTTG
PRIMER_LEFT_1=1260,22
PRIMER_RIGHT_1=1445,22
PRIMER_LEFT_1_TM=59.769
PRIMER_RIGHT_1_TM=59.064
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=1.4642
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=1.438617
PRIMER_LEFT_2_SEQUENCE=ATGAACGGCCTCAACAGACTAT
PRIMER_RIGHT_2_SEQUENCE=AGATTACGTTCGTTGGTGTTGT
PRIMER_LEFT_2=1261,22
PRIMER_RIGHT_2=1444,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=58.561
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=1.4672
PRIMER_LEFT_3_PENALTY=0.531474
PRIMER_RIGHT_3_PENALTY=0.935692
PRIMER_LEFT_3_SEQUENCE=CGTGATGATTCGTCATTACAGC
PRIMER_RIGHT_3_SEQUENCE=AAGATTACGTTCGTTGGTGTTG
PRIMER_LEFT_3=1176,22
PRIMER_RIGHT_3=1445,22
PRIMER_LEFT_3_TM=60.531
PRIMER_RIGHT_3_TM=59.064
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=1.5893
PRIMER_LEFT_4_PENALTY=0.025566
PRIMER_RIGHT_4_PENALTY=1.563720
PRIMER_LEFT_4_SEQUENCE=ATGAACGGCCTCAACAGACTAT
PRIMER_RIGHT_4_SEQUENCE=TATGTCCTGAATCAACCACCTT
PRIMER_LEFT_4=1261,22
PRIMER_RIGHT_4=1495,22
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=58.436
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_00492 Ps_contig_mira-and-tgicl-ass_franssen_5061
SEQUENCE=ACGTTAAAAAATTACATCAATTTTCCATTACTATGATCAACCAATGTAAACGGGATAGTACTGCAACCAGTTGTTAGTAATACAAAAACAGAATATAGAATTCCATCATTTCATCTTTTGTGTGTGTCTTGCTAATCTTTGGATTCAAAATCTTGTTACAAAGGGTGATCAGACATCAGCAATGATACCAGCTAACAAGAAGAATGGGAAGGTGCGTAAAATCGTTTCCCAACAAACTATTGTACAAAATGAATACTACTTTGAAGCTCTGGRAAAAAAGGTCGCTGCCTTCATCATTGAGATGCTAGAGTAGTACTCGTAGTCTCCGGCTTGGTTTCAACAGGGCACCCTCCCTTAACAACTGTGGCGGCCATTCTATTTGGTGGCCAATAGCGGATTATCGATCTACCTATGATATTCTTGGCAGGCAGGGGACCCCACACATGTGAGTCGTCGCTGTTATTGCGGTTGTCTCCCATCACAAAAACGGAGTTTTCTGGCACACGTATGGGTTTCATCTCATACTTCGGTGCCTCAATTATAAACTTCTCATCTCTCTCAACCCCATTAACTACAACATGTCCATTTCGAACCTCCACGATATCACCTTCCTTTGCAACAACGCGTTTTATTAAAACATCATCATCAGTATATTTAACTTCTTGAAGATCTGGAGGGCTTTTAAAAACCACAATATCATTTGCACACGGCTTTCTAAAATAGTATGAAACCTTTTCCGCAACAATTCCATCTCCAATATCATACGTAGGATACATTGACATAGAAGGAATAAACCTCGGTTCAGCAATGAAAGTACGAAAAACAAGTGAAATTGCCAAAGCAGCCAAAACCGTTTTGGCATCATCAGTAGTGAAATTCAACCACTCTGGAAAAAGTTCGGACGACACATCATTCATCTCAACCTCCTGGTCACCATCTCCACCATCACCACCACCACCTCCACCGCCACCATCACCGGAATCTTTCAAAGCTTTATGGCATGGGAATCTACGGCGAAGAGTGGTGGAGGTTTTGTAGAGTTGGGGAAAGTTGGGACGAGAGCGTGGCGGTGAAGAGATTCTAGAACTGAAACGGAGATTGGATAATTTGAGGGAATTGAAGAGATAGGGATGGTCCAAGTTAGGGTTTTGAAGTGATGGTAGTGAAGAAGAGATAGAGAATGAAGATGACATAGTTGAGTTGAATTGAAAACGGTTAAATGAGTTTYC
TARGET=947,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00493 Ps_contig_mira-and-tgicl-ass_franssen_5069
SEQUENCE=GGGATTCTGAGTGCTCTCTCCAATCTCAAATCTCATTCCCTTCACAAAATCTCTGAAGCCACAAACTTGTTCTTTCGCACTTGTATTAATTGCTTCCAAACTCTCTCTCTCTCTCTCTTCTCTCTCTGCCCCAAATATGGAGGCCATCACTGCTTCCTTAGAGAGGTCTCTTCAAAACTGTTCCCTAAACAACCACCACCACCACCACCACCAACAGAACGGAAGGAGACAAGACGACGGTTCCGCCACCACAGATGCACCGGCCGCAGAAGGAGAAGGAGGAGGCATAGGAATCTCATCCACCAG
TARGET=101,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1056
PRIMER_LEFT_PENALTY=0.043988
PRIMER_RIGHT_PENALTY=0.061624
PRIMER_LEFT_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_SEQUENCE=GATTCCTATGCCTCCTCCTTCT
PRIMER_LEFT=61,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.956
PRIMER_RIGHT_TM=60.062
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.1056
PRIMER_LEFT_1_PENALTY=0.043988
PRIMER_RIGHT_1_PENALTY=0.061624
PRIMER_LEFT_1_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_1_SEQUENCE=GAGATTCCTATGCCTCCTCCTT
PRIMER_LEFT_1=61,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=59.956
PRIMER_RIGHT_1_TM=60.062
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.1056
PRIMER_LEFT_2_PENALTY=0.043988
PRIMER_RIGHT_2_PENALTY=0.061624
PRIMER_LEFT_2_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_2_SEQUENCE=ATTCCTATGCCTCCTCCTTCTC
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=59.956
PRIMER_RIGHT_2_TM=60.062
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.1056
PRIMER_LEFT_3_PENALTY=0.043988
PRIMER_RIGHT_3_PENALTY=0.061624
PRIMER_LEFT_3_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_3_SEQUENCE=AGATTCCTATGCCTCCTCCTTC
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=295,22
PRIMER_LEFT_3_TM=59.956
PRIMER_RIGHT_3_TM=60.062
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.1125
PRIMER_LEFT_4_PENALTY=0.043988
PRIMER_RIGHT_4_PENALTY=0.068521
PRIMER_LEFT_4_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_4_SEQUENCE=GTGGATGAGATTCCTATGCCTC
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=302,22
PRIMER_LEFT_4_TM=59.956
PRIMER_RIGHT_4_TM=59.931
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_00494 Ps_contig_mira-and-tgicl-ass_franssen_5069
SEQUENCE=GGGATTCTGAGTGCTCTCTCCAATCTCAAATCTCATTCCCTTCACAAAATCTCTGAAGCCACAAACTTGTTCTTTCGCACTTGTATTAATTGCTTCCAAACTCTCTCTCTCTCTCTCTTCTCTCTCTGCCCCAAATATGGAGGCCATCACTGCTTCCTTAGAGAGGTCTCTTCAAAACTGTTCCCTAAACAACCACCACCACCACCACCACCAACAGAACGGAAGGAGACAAGACGACGGTTCCGCCACCACAGATGCACCGGCCGCAGAAGGAGAAGGAGGAGGCATAGGAATCTCATCCACCAG
TARGET=192,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1056
PRIMER_LEFT_PENALTY=0.043988
PRIMER_RIGHT_PENALTY=0.061624
PRIMER_LEFT_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_SEQUENCE=GATTCCTATGCCTCCTCCTTCT
PRIMER_LEFT=61,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.956
PRIMER_RIGHT_TM=60.062
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.1056
PRIMER_LEFT_1_PENALTY=0.043988
PRIMER_RIGHT_1_PENALTY=0.061624
PRIMER_LEFT_1_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_1_SEQUENCE=GAGATTCCTATGCCTCCTCCTT
PRIMER_LEFT_1=61,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=59.956
PRIMER_RIGHT_1_TM=60.062
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.1056
PRIMER_LEFT_2_PENALTY=0.043988
PRIMER_RIGHT_2_PENALTY=0.061624
PRIMER_LEFT_2_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_2_SEQUENCE=ATTCCTATGCCTCCTCCTTCTC
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=59.956
PRIMER_RIGHT_2_TM=60.062
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.1056
PRIMER_LEFT_3_PENALTY=0.043988
PRIMER_RIGHT_3_PENALTY=0.061624
PRIMER_LEFT_3_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_3_SEQUENCE=AGATTCCTATGCCTCCTCCTTC
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=295,22
PRIMER_LEFT_3_TM=59.956
PRIMER_RIGHT_3_TM=60.062
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.1125
PRIMER_LEFT_4_PENALTY=0.043988
PRIMER_RIGHT_4_PENALTY=0.068521
PRIMER_LEFT_4_SEQUENCE=CAAACTTGTTCTTTCGCACTTG
PRIMER_RIGHT_4_SEQUENCE=GTGGATGAGATTCCTATGCCTC
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=302,22
PRIMER_LEFT_4_TM=59.956
PRIMER_RIGHT_4_TM=59.931
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_00495 Ps_contig_mira-and-tgicl-ass_franssen_5092
SEQUENCE=ACCATGGCATCGGTTTCGCCACCGGCTCGAGGATGTGGTGTTCCGATGGAATTCGGCAACACACAAGCTGCTGTTAGGATTGAAGGTTTTGACTGTCTTAGAAGAGATAGAAGGAGTATCTCTGCTGTAGCCTAATCCATTTTTGAAGAAAGAAAGAAGAACATAGAAAATAGTGAGAGAGAACACAGCTCAATAACCAAGAATCAGCTAGTTTAGTTTTTTTTTTCCTTAGGAAAGAGGATCTAATCTTAATAATTAGTAGTTGTTTGATGTATATATATGTGATGTTTGAAGTTTGATTAGTTAATACAAATAAAAAGAGAAGAAACAAATCAAGAAGTAAAACAAAGGTAAAGAAAAGAAGCCTATTTTT
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2900
PRIMER_LEFT_PENALTY=0.019966
PRIMER_RIGHT_PENALTY=1.270039
PRIMER_LEFT_SEQUENCE=AACACACAAGCTGCTGTTAGGA
PRIMER_RIGHT_SEQUENCE=GCTGATTCTTGGTTATTGAGCTG
PRIMER_LEFT=57,22
PRIMER_RIGHT=207,23
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.270
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=1.3571
PRIMER_LEFT_1_PENALTY=0.087031
PRIMER_RIGHT_1_PENALTY=1.270039
PRIMER_LEFT_1_SEQUENCE=AAGCTGCTGTTAGGATTGAAGG
PRIMER_RIGHT_1_SEQUENCE=GCTGATTCTTGGTTATTGAGCTG
PRIMER_LEFT_1=64,22
PRIMER_RIGHT_1=207,23
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=60.270
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=1.4809
PRIMER_LEFT_2_PENALTY=0.210886
PRIMER_RIGHT_2_PENALTY=1.270039
PRIMER_LEFT_2_SEQUENCE=GCTGCTGTTAGGATTGAAGGTT
PRIMER_RIGHT_2_SEQUENCE=GCTGATTCTTGGTTATTGAGCTG
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=207,23
PRIMER_LEFT_2_TM=59.789
PRIMER_RIGHT_2_TM=60.270
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=1.7105
PRIMER_LEFT_3_PENALTY=0.440473
PRIMER_RIGHT_3_PENALTY=1.270039
PRIMER_LEFT_3_SEQUENCE=CACAAGCTGCTGTTAGGATTGA
PRIMER_RIGHT_3_SEQUENCE=GCTGATTCTTGGTTATTGAGCTG
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=207,23
PRIMER_LEFT_3_TM=60.440
PRIMER_RIGHT_3_TM=60.270
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=1.8226
PRIMER_LEFT_4_PENALTY=0.552581
PRIMER_RIGHT_4_PENALTY=1.270039
PRIMER_LEFT_4_SEQUENCE=ACACAAGCTGCTGTTAGGATTG
PRIMER_RIGHT_4_SEQUENCE=GCTGATTCTTGGTTATTGAGCTG
PRIMER_LEFT_4=60,22
PRIMER_RIGHT_4=207,23
PRIMER_LEFT_4_TM=59.447
PRIMER_RIGHT_4_TM=60.270
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_00496 Ps_contig_mira-and-tgicl-ass_franssen_5093
SEQUENCE=GTACGTACCTACTTACNGTGGCGTCGTCNCNNCGGCTCGAGGATGTGGTGTTCCGATGGAATTCGGCAACACACAAGCTGCTGTTAGGATTGAAGGTTTTGACTGTCTTAGAAGAGATAGAAGGAGTATCTCTGCTGTAGCCTAATCCATTTTTGAAGAAAGAAAGAAGAACATAGAAAATAGTGAGAGAGAACACAGCTCAATACCAAGAATCAGCAGTAGTTTTTTTTCCTTTGKAARRAGGATCTAATCTTAATAATTAGTAGTGTTGTNTGTATATATAATTRTGNTGTTGTAAGTTGT
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00497 Ps_contig_mira-and-tgicl-ass_franssen_5094
SEQUENCE=CCTTTTGGTAAAGTAGGATCTAATCTTAATAATTAGTAGTGTTTGATAGTAATAATAATAATTGTGATGTTTGAAGTTTGATGTTAATCAATAAAAGAGAGAACAAATCAGAGTAAACAAGGTAAGAAAAGAAGCCATTTTCTTCTGTTTKKTTATTAGGTTTGTGGAGAGTTAAGGTATCCTTCCTNTGTAATTGTTAATTTGGAGGAAATTGGTAATTTTGTTACATCCTATTATTGTTATTAATCATAATGTAGTTTTAAAAAGT
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00498 Ps_contig_mira-and-tgicl-ass_franssen_5095
SEQUENCE=CGTTTTTTTAAATTTACTTGTATAAATATAAAGTTAGATTTGTTATCCATTATTACTTTACTATGCACACGTATTATAATAAAAATAAAAATAAAATAAATAATATAAAGGGTTTGGTTATCTTTATGTAAACGACCTATGTGAATGGTGGGGCAATCAAAARSTKWCTCCACAAAGACTTGCTTTTTAATTTCTTAATAATAAAATATACACCCCCAATTATATTAAAATAATAATTAAACARATAATGCATTATTGGTTCTTCAATTTGTTTTTTTATGGACCCAAACCGGAAAAGAAGGAGCTTTCTTATATACCCTTTCCTTTTCTTTTCCATTTAATAATCATTCTTTCTCCAAAGGCTCCTATTCTTTTTTTCAACTTTATTGACAAAAAGAATTATTATAGAATAGTAACAACTATGTTTTTTTCCCACCAAATAGGGTTTGTATTGTATTGAAATTTGAATATGTACCAATTTGAATCCATTAGAATGAAGAACCAAAAGGTGCCCTATGAGGAATACCTTTTCTTCTTCTTGCATTTAAAGGAGGAGGAGGAGGTGTGATAGGATACATATCTTGTGTTGAACCTTCACGCAATTCTTCTTCTGATCTACTACTTGACACCCCCTCCATTCTCATTGCTTCATTCTTACCCCCTTCAATAGCAAAACTTGCATCTCCATTTTTCTTTATTCTTTCAAGCTTACATCCCAAAGAACAGAACCTAAATGAATCCAAGAGACTCCTTCCACAAATCTCACAATGTGAGCTACTCCTTTACAGATTTTGGTTGAGGCCTCACATTCAAGAACAATA
TARGET=549,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00499 Ps_contig_mira-and-tgicl-ass_franssen_5110
SEQUENCE=CAGAGAGAATCATTTGAAGATGTTTAAATTAGAAGATGTTGCAATGGGAATCTGGATCGCAGAAATGAAGAAGGAAGGTCTAAAAGTTCGATACGAAAATGAGGGCAGAGTTTATAACGAGGGTTGCAAGGATGGTTATGTGGTTGCCCATTATCAAGGTCCAAGGGAGATGCTATGTTTGTGGCACAAACTTGAGGAATTAAAACGTGCAACATGCTGTGGTGATAGGAGGTGACTACTGAATGCTGACTAACCAAGATCCCACTTCTGAATAAAAGWAACTTTTCCCCWTACTTTTTTTTAGTAATAAATAAATAGTAGAATAGTTAGCTAGCCAGATATGATTAGGAATAGGTTAA
TARGET=306,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00500 Ps_contig_mira-and-tgicl-ass_franssen_5113
SEQUENCE=TTTTTTTTAGTAACAGAAGCAAGATTATCCATTACTGAATAAAATAGCATTCATTTTACAACATTTGATAGGGATTCTTCCTCACACACACAAAAATAACAGCAACTAGCAACTAATAATCAAGGTTCTGCAATTCTTGCAAGGCTCTACAGTTTTTGATATTCGCGGTTATATGCAGCGCGATCAGAGACAGACATCAAAACCCTTGATGTTATATGTGACTGATCATCAGAGACATCAAAACCCTTGATGTTATTATATGCGACGATCATAGACATCAATCGCAGCAAACCTATTTTGAAACCATACTAAGACTAATAACTCTTCGGGCTTAAAATTTCCCTAATACTAGCAAGGCATGGATCCAAAAAACCATAAATCCAAATGCCTTGGAGGAATACACACTATGTGACTTGATCTTCAAAGATATATCTTCATTCTCACAAGTATATATACTGCTTCATCATATTACACCAGACCTACACTGTACAAGGATGAAGTCAGAAGATGCGGTTGCATACAAACACTGCCGCATCATCTCCCAGCACCGGTTAGGGTTCTCTCCAACACTTCAGAATTCGATGTCAATGAAGGTAGAGGAATCGACTCCGAACTCGCAGGTGCAGCTTCGGTCCCCAACTCTTCATTTACAGGCAGTGGTTGAGGCGAGTATGTAAAGGAAAGATCTTTGTTTTCGGATAGAGCTATGAGCTCTTTCTCCTCTAACCCTTTGGTTGGCCCCAAGCTGCACCTTTCTGGAGTGTGTTTGGCCAATGCATCTCTAAACTTCTTGATCGTGGCATTGGTGCAGCTAAAGCTGCATACTCGACCCTGAGCGCCTCTATAGAACCGGAAAAACGGAAGGACATGAACATTAAGACTGTAACACATAGATTTATGCTCCTCATAGTTCACAGAAAGGAACTCAACATCAGGATTCATCTCTGCAAATTGACATATCTTAGGATGAAGGGCTTTGCAGCCACCACAACCAGGAGAGAAAAAATCAACAATCACAAGTTTGTCCCCTGCGTTTAAAAGTGAATCTACAAGATCTTGTGCTGAAGTCACCTCCCTCATGTTAGGTTGAAGACCCTTTTCCCACCATTTTTGAGCTTTCCCTATTCTAAGTGTCATCTTAGGAGCAGCTGAAACTGAAAACTGTGAATTGATCCTTTTGGATGGTATTGATCTAGTTACACGAAAGAGAGGCTTTTTGCCATGAAACTCAGATGAAAAAGAAGATCTGAGAAACTGAGATTTCAGCTTCAAAGATGGAAAACTTCCGACACCAGTCTTGGGAACCATGGAAGCCCTTTGAAGCTGTTGATTTTTGTTTCCATGCCAAGAAGAAACCAAGCTTGTCTTGATCAAAGTTTCAGCCATTAAATATAAATTTTTCTTCACAAAAAAAAAGCTATTCGGTTACGTTTTGTTTCAGATTTTCTCGAGAATAGAAATTGATAAGATGAGAGAAAGAAAATGAAGACTTTTTTGTTCTCCCGACGTACTCTGCGTTGKAGGATTTATCCACCC
TARGET=83,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00501 Ps_contig_mira-and-tgicl-ass_franssen_5118
SEQUENCE=TGACAAAGAACATTGGATCATAACAAACTAAACATATCAGTTTCAGATAAACATCAACAAAGACAGGGCCAACGTGACCTAGTCTAAACACCCAAGTAATAGCTTTCATCTCACTGCTGCTCTAGCACTCATTGATGTGTGATAATTTGTGCTTCTCTGCTTCCGGTAAGATGTATATACATCATCATACTGACTCACTTCATTTGGATCAGCCGTGGCCCCAAGTCCCAATCTTCCCGAACCACTTGATCCTGGCTTTCTGTCGACACTCCCATCAGCATAGGTCCCTGTTTCTGGATCAATTTGCTGCAAGTTTGGGGGTGGAGGTATGCAAGCACCTCCCTTTCGTACTTGAGATTCTCTCTCGTATTCCCCATCGTATTCATCATCATCAT
TARGET=383,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00502 Ps_contig_mira-and-tgicl-ass_franssen_5123
SEQUENCE=AAAAGGAAGATTTGAAGCAGAACCTATGTGTTTTGTAGTAARATCATCTATACATATGTGTTCATCTTCCATATATTGTTTAGGCCCTCTCTCAGCACAACTTCCTGATCGAAATATGGGTAGATAATCTGTCTTGTCTTGAGAAGATGACTTACTTCCAGTTACCCCGGCATCTACACCCAAATTTGCTACAGCCTGCATCAATCTGGTTGAGCTAAGGCTGTGTCTCAAACCAGACACATGCCTTGGAGGTTTCCCCTTTGTGATTGGTTTCAGATTCTTAAAATCCTCTGATTTATCGTCATCATCATCATCAATAACATCAACATCATCATCATCATGAACAACAACCAGAAAATCATTGTTTTTATTGTAATCATCGCTTTCAAACATTGTAAATGGAGGAGAATAATAATCTACACCCTCAAGCATAATAGTTTCTTCCAAATCGTACACACCTAACTAACAAAAACCAATAAAAGAAGAGTCTTTTAAGGGAAAAAATGTTTCAATTGGAAGAATAAGTTAAACCGAAATCGATAAATAAAGTGCCTAACCGTGATAATTTAAGAAAGAGAAACAGATTGAAGATGGTGAATAGGAAGGAATTAGG
TARGET=302,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00503 Ps_contig_mira-and-tgicl-ass_franssen_5123
SEQUENCE=AAAAGGAAGATTTGAAGCAGAACCTATGTGTTTTGTAGTAARATCATCTATACATATGTGTTCATCTTCCATATATTGTTTAGGCCCTCTCTCAGCACAACTTCCTGATCGAAATATGGGTAGATAATCTGTCTTGTCTTGAGAAGATGACTTACTTCCAGTTACCCCGGCATCTACACCCAAATTTGCTACAGCCTGCATCAATCTGGTTGAGCTAAGGCTGTGTCTCAAACCAGACACATGCCTTGGAGGTTTCCCCTTTGTGATTGGTTTCAGATTCTTAAAATCCTCTGATTTATCGTCATCATCATCATCAATAACATCAACATCATCATCATCATGAACAACAACCAGAAAATCATTGTTTTTATTGTAATCATCGCTTTCAAACATTGTAAATGGAGGAGAATAATAATCTACACCCTCAAGCATAATAGTTTCTTCCAAATCGTACACACCTAACTAACAAAAACCAATAAAAGAAGAGTCTTTTAAGGGAAAAAATGTTTCAATTGGAAGAATAAGTTAAACCGAAATCGATAAATAAAGTGCCTAACCGTGATAATTTAAGAAAGAGAAACAGATTGAAGATGGTGAATAGGAAGGAATTAGG
TARGET=327,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00504 Ps_contig_mira-and-tgicl-ass_franssen_5127
SEQUENCE=GGTTCAATACTATAATTTTATTTATGAATAAAACACTAGTATAGTATAGTATCAAACTATGAATCAATATTATTACAACAACAAGAAGAACTTAACAAGTATTGAAACTCAAAATCAAAGACAAGCAGGACAATGAACTAACCCATTCTCATTACACTTAGGACACCTCTGCAGTGTCGAATCACTTATTTCAACCCCACTAATCACCAACTTACAGCTTCCACCACAATCCAAACAAGGAACAAACCTAGCCCCACCACACCCCCCACAACCTTGTCTCCCAACACCTCTCTCCACACGCGTCGCATTCAATATCCTCCCAAGTCTCCCCGTTTCATTTAACTCCGTCAACTCATCCAAACCACCCAAATATCTCCCTTTCACAAACACCCGCGGCAAAACCACCCCAACCCCCGACTCTTCCTCCGTTAACAACAACTCTTTCACTTCCTTCAAAAACTCTCCGTGAAGTGCCACGTCACGCTCGTCGAATATCACCCTGTGATTCTCTAACAAGTCCCGAGCTTTGTTGCAGTTATCGAAAGTTTTTCTAACTCCTCTTAACGACGTCGTGTATATCACCACGGCGTCGTTTCCTTTCGGTGGAACTTTCTCCGGGTAGTTACTCAGTGGTCCCTCCTTAACCGTTCGAAATTCGATTCTCTCTCTTCTTTTTGTTTCTCTAACATGTCTTTCTGTA
TARGET=37,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00505 Ps_contig_mira-and-tgicl-ass_franssen_5128
SEQUENCE=GACATGTACTACTACTACAACACKAGTATATGTGAGGGAGAGAGTCACTGCAACTAGCTATCATCCAAAGAACGAAATGGGGTGTGCATCTTCCAAAGGTATCGACGGCACGGCAGTCGCCGTATACCAGCCACCACCGACGAGCTTCGCCGTGTTCGACATAAACTCCATAGAAGAGCCATGGCTCAAACACCTCAACGACAACACCAACACCATGTCACAAAGAAAACAAACCCGCTCTCCCTCAACCTATTCTCCA
TARGET=7,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00506 Ps_contig_mira-and-tgicl-ass_franssen_5137
SEQUENCE=TGCTTAAGATTACTAATAATCTCGACTTCTATTTACAAAACTCGACATCCCCTTGAAAATCGGATTCTAAAACCCGCTTAACCGCAACAACAGATCCATCAGCCAAAGTTCCTTTGAAAACCAAACCAAACCCACCTCTCCCTATGAAATGTCTTGGATGAAAAATTATCAGTTGCTTTCTCAAGTTCCGGAATTTTGTACCAAATCGACCCGGTATTCGGTCTCACCTACGGCGAGAGTCTTGTTCCTCGGGATCAAATCAATTGAAAGTTCAGCTTCTCTTCC
TARGET=118,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0421
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=1.037717
PRIMER_LEFT_SEQUENCE=AACAGATCCATCAGCCAAAGTT
PRIMER_RIGHT_SEQUENCE=CGGAACTTGAGAAAGCAACTG
PRIMER_LEFT=88,22
PRIMER_RIGHT=189,21
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=1.1663
PRIMER_LEFT_1_PENALTY=0.128591
PRIMER_RIGHT_1_PENALTY=1.037717
PRIMER_LEFT_1_SEQUENCE=TTTACAAAACTCGACATCCCCT
PRIMER_RIGHT_1_SEQUENCE=CGGAACTTGAGAAAGCAACTG
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=189,21
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=60.038
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=1.1663
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=1.037717
PRIMER_LEFT_2_SEQUENCE=TTACAAAACTCGACATCCCCTT
PRIMER_RIGHT_2_SEQUENCE=CGGAACTTGAGAAAGCAACTG
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=189,21
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=60.038
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=158
PRIMER_PAIR_PENALTY_3=1.2252
PRIMER_LEFT_3_PENALTY=0.128591
PRIMER_RIGHT_3_PENALTY=1.096569
PRIMER_LEFT_3_SEQUENCE=TTACAAAACTCGACATCCCCTT
PRIMER_RIGHT_3_SEQUENCE=CATCCAAGACATTTCATAGGGA
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=159,22
PRIMER_LEFT_3_TM=59.871
PRIMER_RIGHT_3_TM=58.903
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=128
PRIMER_PAIR_PENALTY_4=1.2252
PRIMER_LEFT_4_PENALTY=0.128591
PRIMER_RIGHT_4_PENALTY=1.096569
PRIMER_LEFT_4_SEQUENCE=TTACAAAACTCGACATCCCCTT
PRIMER_RIGHT_4_SEQUENCE=TCATCCAAGACATTTCATAGGG
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=160,22
PRIMER_LEFT_4_TM=59.871
PRIMER_RIGHT_4_TM=58.903
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=129
=
PRIMER_SEQUENCE_ID=SSR_00507 Ps_contig_mira-and-tgicl-ass_franssen_5147
SEQUENCE=CTTTTGATGCTAAGAGTTAAGCATGAATCTCTTGCCCAGACTCAAAGGAATTTTTTGGGAGAAGAACTCAATACACTAAGCATAAAAGATTTGCAGAATATAGAAAAACAACTGGAGAGAACGCTTGCACAAGCTCGAAAGCACCAAATGCAGAAACTGATGACACGAGTTGATGAATTAGGCCGAGAGGTTCACAAGGTGGAGGAGGTTAACAAACAGTTAGAATCCAAGTCAGTTTCGGCACCAACAAGCTGGTTCAAAAGGACGGGCAACATATACAAGGATTGACCGAGGCCAGAGTAGTCGCAACAAGAACAATGGATGGATGAATATTTGAACTTTCATTTACCTAAGGCTTGAGACCCCTAAAACATGTCTCAAATAATCACTAATTTATGAGAGCCTATTTGGTTTAATTTATAATAGAACTTCTTCTTTCTTTCTTTTCTWRCAYGAYARTRWSGCKTCARGTTGGGTCGGTTT
TARGET=433,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00508 Ps_contig_mira-and-tgicl-ass_franssen_5155
SEQUENCE=CAATCTATCAACCTGCAACAACAACAACAACAATAACGTGCAATAACATCATCATCATCAACATGAAAACAGAACATGCAAATGAATACGATGAGAATCAAGTCGAAGAAGAAACCAATTGATGATGAGAATCGAAGTTGACAAGATCGTGAGTAGGATGTGGCTGAATGACGAGTTCATCGGAAGGACGAAGGTGAACGGTGGCATAGAAATTGGAAAGACTGAAAGCGATTGTTTTGCCGTAATAGACATCGCTTGGATTTCCGAGAAAACCAA
TARGET=16,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00509 Ps_contig_mira-and-tgicl-ass_franssen_5155
SEQUENCE=CAATCTATCAACCTGCAACAACAACAACAACAATAACGTGCAATAACATCATCATCATCAACATGAAAACAGAACATGCAAATGAATACGATGAGAATCAAGTCGAAGAAGAAACCAATTGATGATGAGAATCGAAGTTGACAAGATCGTGAGTAGGATGTGGCTGAATGACGAGTTCATCGGAAGGACGAAGGTGAACGGTGGCATAGAAATTGGAAAGACTGAAAGCGATTGTTTTGCCGTAATAGACATCGCTTGGATTTCCGAGAAAACCAA
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0402
PRIMER_LEFT_PENALTY=0.019966
PRIMER_RIGHT_PENALTY=0.020269
PRIMER_LEFT_SEQUENCE=AACCTGCAACAACAACAACAAC
PRIMER_RIGHT_SEQUENCE=CGATGTCTATTACGGCAAAACA
PRIMER_LEFT=9,22
PRIMER_RIGHT=253,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.020
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=245
PRIMER_PAIR_PENALTY_1=0.0777
PRIMER_LEFT_1_PENALTY=0.057411
PRIMER_RIGHT_1_PENALTY=0.020269
PRIMER_LEFT_1_SEQUENCE=ATCAACCTGCAACAACAACAAC
PRIMER_RIGHT_1_SEQUENCE=CGATGTCTATTACGGCAAAACA
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=253,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.1806
PRIMER_LEFT_2_PENALTY=0.019966
PRIMER_RIGHT_2_PENALTY=0.160609
PRIMER_LEFT_2_SEQUENCE=AACCTGCAACAACAACAACAAC
PRIMER_RIGHT_2_SEQUENCE=AGCCACATCCTACTCACGATCT
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=164,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=60.161
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.2180
PRIMER_LEFT_3_PENALTY=0.057411
PRIMER_RIGHT_3_PENALTY=0.160609
PRIMER_LEFT_3_SEQUENCE=ATCAACCTGCAACAACAACAAC
PRIMER_RIGHT_3_SEQUENCE=AGCCACATCCTACTCACGATCT
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=164,22
PRIMER_LEFT_3_TM=59.943
PRIMER_RIGHT_3_TM=60.161
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=0.2520
PRIMER_LEFT_4_PENALTY=0.019966
PRIMER_RIGHT_4_PENALTY=0.232060
PRIMER_LEFT_4_SEQUENCE=AACCTGCAACAACAACAACAAC
PRIMER_RIGHT_4_SEQUENCE=AACAATCGCTTTCAGTCTTTCC
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.980
PRIMER_RIGHT_4_TM=59.768
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_00510 Ps_contig_mira-and-tgicl-ass_franssen_5172
SEQUENCE=TTAATAATTTAAATTACTTTTTCTTTTAAAAACCCTAAAACCTTTCTTCCTAAATAAGCATCTAACACTACGCAAATACCCTACCCTTTCCAAAGACCTTCCTTCCCTTCATGAACTGAAAGAAGAAGCTCCTCTAGTTCTAGCAAGATACTCATGAGGAGGTAAACGAATTTCTCCACCATAGCCATCTTCATCTTCATCATCATCACTACTCTCTTTGTTCTTCTTCTTCTTCTTGTATTCATCTTTCAAAATCTTTGACCAATCTGGTATATTCATCGGTAAAGAAGATGAACCTATAGGGTTAACTCGGTTATTGGATTCCATTTTTCTAGAAACTCTCTTCAAACCTGGTAGCACACACTTTTTTGATTCTGTCATGTTGTCGGAATTTGAACATGACATGTTCCATACATCAGCTTCATCGAGCTCGAACTCAGTTGCTTCGGATGATTTTGTGTTTATCTCTGAGGGTGTTGGAAAAATGTAACTTGATCTTGAAAGAAAACTCTTCCTAGAAGAAGCCATTGGAAGTGGTTTTGGTGTTTTTGTTTTTGTTTTTGTTTTTGTTACTATGTTTTCATTTTGTTCTGTTTTTTTGGCTTATATTGTACACTCTGGTCTCACTCTACGCGTTATGATAAATMAAHGAMACAAGTAATAATAGTAAATAC
TARGET=197,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00511 Ps_contig_mira-and-tgicl-ass_franssen_5172
SEQUENCE=TTAATAATTTAAATTACTTTTTCTTTTAAAAACCCTAAAACCTTTCTTCCTAAATAAGCATCTAACACTACGCAAATACCCTACCCTTTCCAAAGACCTTCCTTCCCTTCATGAACTGAAAGAAGAAGCTCCTCTAGTTCTAGCAAGATACTCATGAGGAGGTAAACGAATTTCTCCACCATAGCCATCTTCATCTTCATCATCATCACTACTCTCTTTGTTCTTCTTCTTCTTCTTGTATTCATCTTTCAAAATCTTTGACCAATCTGGTATATTCATCGGTAAAGAAGATGAACCTATAGGGTTAACTCGGTTATTGGATTCCATTTTTCTAGAAACTCTCTTCAAACCTGGTAGCACACACTTTTTTGATTCTGTCATGTTGTCGGAATTTGAACATGACATGTTCCATACATCAGCTTCATCGAGCTCGAACTCAGTTGCTTCGGATGATTTTGTGTTTATCTCTGAGGGTGTTGGAAAAATGTAACTTGATCTTGAAAGAAAACTCTTCCTAGAAGAAGCCATTGGAAGTGGTTTTGGTGTTTTTGTTTTTGTTTTTGTTTTTGTTACTATGTTTTCATTTTGTTCTGTTTTTTTGGCTTATATTGTACACTCTGGTCTCACTCTACGCGTTATGATAAATMAAHGAMACAAGTAATAATAGTAAATAC
TARGET=221,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00512 Ps_contig_mira-and-tgicl-ass_franssen_5173
SEQUENCE=GGGTTTTAGGGGGTAAACGGGGTTAAAAATTAAAACCGTAAAGGTTAACGGGTTTAACGGTTAAACTTTTACCTTGGTAAATTAAGTTAGAGTTGTTTACCAAACCCGTAACCGTTTAGTTATCTAGTTAGAAATAATAATTAATTAATTCTTACCAAAACCTAACTTTCCTTACCTAAATAGMATTCCTAACACTACGCAAATCCCTTACCTTTCTAAAGTCCTTCCTTTTCCCTTTCATGAACTGAAAGAGAAGCTCCTCTAGTTCTAGCAAGATACTCATGAGGGAGGTAAACGAATTTCTCCACCATAGCCATCTTCATCTTCATCATCATCACTACTCTCTTTGTTCTTCTTCTTCTTCTTGTATTCATTCTTTACAAATTCTTGACCATCTGTATATTCATACGGTAAAAGAAGATAGAACCTTAAGTTAGGGGTTAACTTCGGTTAG
TARGET=138,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00513 Ps_contig_mira-and-tgicl-ass_franssen_5173
SEQUENCE=GGGTTTTAGGGGGTAAACGGGGTTAAAAATTAAAACCGTAAAGGTTAACGGGTTTAACGGTTAAACTTTTACCTTGGTAAATTAAGTTAGAGTTGTTTACCAAACCCGTAACCGTTTAGTTATCTAGTTAGAAATAATAATTAATTAATTCTTACCAAAACCTAACTTTCCTTACCTAAATAGMATTCCTAACACTACGCAAATCCCTTACCTTTCTAAAGTCCTTCCTTTTCCCTTTCATGAACTGAAAGAGAAGCTCCTCTAGTTCTAGCAAGATACTCATGAGGGAGGTAAACGAATTTCTCCACCATAGCCATCTTCATCTTCATCATCATCACTACTCTCTTTGTTCTTCTTCTTCTTCTTGTATTCATTCTTTACAAATTCTTGACCATCTGTATATTCATACGGTAAAAGAAGATAGAACCTTAAGTTAGGGGTTAACTTCGGTTAG
TARGET=326,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00514 Ps_contig_mira-and-tgicl-ass_franssen_5173
SEQUENCE=GGGTTTTAGGGGGTAAACGGGGTTAAAAATTAAAACCGTAAAGGTTAACGGGTTTAACGGTTAAACTTTTACCTTGGTAAATTAAGTTAGAGTTGTTTACCAAACCCGTAACCGTTTAGTTATCTAGTTAGAAATAATAATTAATTAATTCTTACCAAAACCTAACTTTCCTTACCTAAATAGMATTCCTAACACTACGCAAATCCCTTACCTTTCTAAAGTCCTTCCTTTTCCCTTTCATGAACTGAAAGAGAAGCTCCTCTAGTTCTAGCAAGATACTCATGAGGGAGGTAAACGAATTTCTCCACCATAGCCATCTTCATCTTCATCATCATCACTACTCTCTTTGTTCTTCTTCTTCTTCTTGTATTCATTCTTTACAAATTCTTGACCATCTGTATATTCATACGGTAAAAGAAGATAGAACCTTAAGTTAGGGGTTAACTTCGGTTAG
TARGET=350,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00515 Ps_contig_mira-and-tgicl-ass_franssen_5178
SEQUENCE=GKTCTTAATTTAATCATGTAATTAAGAAAAGCCAGAAAGATAGAAACATAATAATTCTGAAAGATCATCCATATATTCGGTTGGTGCGAACAATGATCATTCAGAAAACTCATAATTCAAGCATCACGAAAAGTGATATGGAGGTAGAGAATTCAACATTATGTTGATCGATTCGATTGATTAATTCACATATACGATTCAGATCCAATATTTCACATCTTATACAAAGTGAAATAGCTATATATATAATTAACATATAAAAGAACAAAAAGAAAGAAAGAAATGGATCATGATGATGAAAGAAGAGGAAATCGATAAAAGAAGAAAAATCGTGTAATTAATTTACGTAATTTAACCAACGAGCACCAAGGCAGTGTTCGAGACTGAATTTCAGAGAGAGTTTGATCAATTTTCATGTACAAGGACAAGGCCTCTTTGGAAAAGTGGATCTGAAAAACGCATCCATGTCTTCATTACTCGAAACCTCGAAGAATTGAAATTCATCCATAAGCTTCCTCTTACAAGAAACGAACGGCTTCGGTTTCTTCGGCGCCGGCGGACAATTCAAGATCTCCGGTATCTTGCTTTCCTTCGAAGTCGGAGTCCGGTAASTCKGAGTCCG
TARGET=239,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00516 Ps_contig_mira-and-tgicl-ass_franssen_5178
SEQUENCE=GKTCTTAATTTAATCATGTAATTAAGAAAAGCCAGAAAGATAGAAACATAATAATTCTGAAAGATCATCCATATATTCGGTTGGTGCGAACAATGATCATTCAGAAAACTCATAATTCAAGCATCACGAAAAGTGATATGGAGGTAGAGAATTCAACATTATGTTGATCGATTCGATTGATTAATTCACATATACGATTCAGATCCAATATTTCACATCTTATACAAAGTGAAATAGCTATATATATAATTAACATATAAAAGAACAAAAAGAAAGAAAGAAATGGATCATGATGATGAAAGAAGAGGAAATCGATAAAAGAAGAAAAATCGTGTAATTAATTTACGTAATTTAACCAACGAGCACCAAGGCAGTGTTCGAGACTGAATTTCAGAGAGAGTTTGATCAATTTTCATGTACAAGGACAAGGCCTCTTTGGAAAAGTGGATCTGAAAAACGCATCCATGTCTTCATTACTCGAAACCTCGAAGAATTGAAATTCATCCATAAGCTTCCTCTTACAAGAAACGAACGGCTTCGGTTTCTTCGGCGCCGGCGGACAATTCAAGATCTCCGGTATCTTGCTTTCCTTCGAAGTCGGAGTCCGGTAASTCKGAGTCCG
TARGET=269,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00517 Ps_contig_mira-and-tgicl-ass_franssen_5180
SEQUENCE=AAGACAGTGGTATCAACGCAGAGAACCTTCCTTTCCCTCTGTTTTTGTCATCAAAATTAAAGTCATTGTGGTGTTTGTTTGTTTAAAGTCAGTCGTTTAAAATTTCTCATTGTGAAAAACTAAA
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00518 Ps_contig_mira-and-tgicl-ass_franssen_5181
SEQUENCE=CGTCTCGGCTTTCTCCCAAGAAAGACGAGCTGCTCGTCATCGCGGAAAAGTAGAAAAGACCTTTTCCCTAAAACGAATGATCCTACCTAAACCATGCAGCTTGACTGCTTTTGTTGGCTACAAGTCTGGGATGACCCATATTGTTAGGGAGGTTGAGAAGCCTGGATCCAAGCTTCATAAGAAAGAGACATGTGAAGCTGTCACTATCATTGAAACTCCTCCACTTGTTGTTGTTGGAGTGGTGGGATATGTGAAAACACCGCGAGGCCTTCGCACACTGAATACTGTTTGGGCTCAGCATCTAAGTGAAGATATCAAGCGTCGGTTTTACAAGAACTGGTGCAAGTCAAAGAAGAAGGCTTTTACCAAGTATTCCAAAAAGTATGAAACTGAAGAAGGAAAGAAGGATATTCAATCTCAGCTTGAGAAATTGAAAAAATATGCTACTGTCATCCGTGTTCTTGCTCATACTCAGATAAGGAAGTTGAAGGGTCTAAAACAAAAGAAAGCTCATTTGATAGAGATCCAAGTTAATGGTGGAACAATAAGCCAAAAGGTGGATTATTCATACAGTTTCTTTGAAAAGCAAGTTCCTGTCAATGCTGTTTTTCAGAAGGATGAGATGATTGATATCATTGGAGTAACTAAAGGTAAAGGTTATGAAGGTGTGGTAACTCGTTGGGGTGTGACCCGCCTTCCACGGAAGACACACAGAGGTCTAAGGAAGGTTGCTTGTATTGGTGCTTGGCACCCTGCTCGTGTTTCTTTCACAGTTGCCAGGGCTGGTCAGAATGGATACCATCATCGTACTGAATTGAACAAGAAGATCTACAAGGTTGGCAAGACTGACGAAGAGTCACACACAGCCATCACAGAATTTGACAGGACTGAGAAGGATATTACTCCCATGGGTGGATTTCCTCATTACGGTATAGTGAAGCATGACTATCTTATGGTGAAGGGAGGCTGTGTTGGACCAAAGAAGCGGGTTGTGACTCTTCGTCAATCACTACTGAAGCAAACGTCTCGATTGGCTCTTGAAGAGATCAAGTTGAAATTCATTGACACCTCCTCCAAGTTTGGACATGGTCGCTTCCAGACTACTCAGGAGAAACAGAAGTTTTATGGTCGTTTGAAGGCCTAGTTCTTATTTTATTATATTTATTGTTTATTGTTTATTGTGTCGAGAGAACCTTCCTTTTCCCTCTGTTTTTGCATCAAATTAAGTCATTGTGGTGTTTGTTTGTTTAAAGTCAGTCGTTTAAATTTCTCATTGTGAAAAACTAATTTTGTTTTACTTTGATGGGTTTGTGTATTTGCCTT
TARGET=225,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0556
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.048692
PRIMER_LEFT_SEQUENCE=GATCCTACCTAAACCATGCAGC
PRIMER_RIGHT_SEQUENCE=CTTTGACTTGCACCAGTTCTTG
PRIMER_LEFT=78,22
PRIMER_RIGHT=351,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.951
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.0678
PRIMER_LEFT_1_PENALTY=0.006951
PRIMER_RIGHT_1_PENALTY=0.060824
PRIMER_LEFT_1_SEQUENCE=GATCCTACCTAAACCATGCAGC
PRIMER_RIGHT_1_SEQUENCE=AGCCTTCTTCTTTGACTTGCAC
PRIMER_LEFT_1=78,22
PRIMER_RIGHT_1=360,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=60.061
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.1025
PRIMER_LEFT_2_PENALTY=0.006951
PRIMER_RIGHT_2_PENALTY=0.095538
PRIMER_LEFT_2_SEQUENCE=GATCCTACCTAAACCATGCAGC
PRIMER_RIGHT_2_SEQUENCE=TAGATGCTGAGCCCAAACAGTA
PRIMER_LEFT_2=78,22
PRIMER_RIGHT_2=303,22
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.1170
PRIMER_LEFT_3_PENALTY=0.068302
PRIMER_RIGHT_3_PENALTY=0.048692
PRIMER_LEFT_3_SEQUENCE=CCCATATTGTTAGGGAGGTTGA
PRIMER_RIGHT_3_SEQUENCE=CTTTGACTTGCACCAGTTCTTG
PRIMER_LEFT_3=134,22
PRIMER_RIGHT_3=351,22
PRIMER_LEFT_3_TM=60.068
PRIMER_RIGHT_3_TM=59.951
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.1291
PRIMER_LEFT_4_PENALTY=0.068302
PRIMER_RIGHT_4_PENALTY=0.060824
PRIMER_LEFT_4_SEQUENCE=CCCATATTGTTAGGGAGGTTGA
PRIMER_RIGHT_4_SEQUENCE=AGCCTTCTTCTTTGACTTGCAC
PRIMER_LEFT_4=134,22
PRIMER_RIGHT_4=360,22
PRIMER_LEFT_4_TM=60.068
PRIMER_RIGHT_4_TM=60.061
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_00519 Ps_contig_mira-and-tgicl-ass_franssen_5181
SEQUENCE=CGTCTCGGCTTTCTCCCAAGAAAGACGAGCTGCTCGTCATCGCGGAAAAGTAGAAAAGACCTTTTCCCTAAAACGAATGATCCTACCTAAACCATGCAGCTTGACTGCTTTTGTTGGCTACAAGTCTGGGATGACCCATATTGTTAGGGAGGTTGAGAAGCCTGGATCCAAGCTTCATAAGAAAGAGACATGTGAAGCTGTCACTATCATTGAAACTCCTCCACTTGTTGTTGTTGGAGTGGTGGGATATGTGAAAACACCGCGAGGCCTTCGCACACTGAATACTGTTTGGGCTCAGCATCTAAGTGAAGATATCAAGCGTCGGTTTTACAAGAACTGGTGCAAGTCAAAGAAGAAGGCTTTTACCAAGTATTCCAAAAAGTATGAAACTGAAGAAGGAAAGAAGGATATTCAATCTCAGCTTGAGAAATTGAAAAAATATGCTACTGTCATCCGTGTTCTTGCTCATACTCAGATAAGGAAGTTGAAGGGTCTAAAACAAAAGAAAGCTCATTTGATAGAGATCCAAGTTAATGGTGGAACAATAAGCCAAAAGGTGGATTATTCATACAGTTTCTTTGAAAAGCAAGTTCCTGTCAATGCTGTTTTTCAGAAGGATGAGATGATTGATATCATTGGAGTAACTAAAGGTAAAGGTTATGAAGGTGTGGTAACTCGTTGGGGTGTGACCCGCCTTCCACGGAAGACACACAGAGGTCTAAGGAAGGTTGCTTGTATTGGTGCTTGGCACCCTGCTCGTGTTTCTTTCACAGTTGCCAGGGCTGGTCAGAATGGATACCATCATCGTACTGAATTGAACAAGAAGATCTACAAGGTTGGCAAGACTGACGAAGAGTCACACACAGCCATCACAGAATTTGACAGGACTGAGAAGGATATTACTCCCATGGGTGGATTTCCTCATTACGGTATAGTGAAGCATGACTATCTTATGGTGAAGGGAGGCTGTGTTGGACCAAAGAAGCGGGTTGTGACTCTTCGTCAATCACTACTGAAGCAAACGTCTCGATTGGCTCTTGAAGAGATCAAGTTGAAATTCATTGACACCTCCTCCAAGTTTGGACATGGTCGCTTCCAGACTACTCAGGAGAAACAGAAGTTTTATGGTCGTTTGAAGGCCTAGTTCTTATTTTATTATATTTATTGTTTATTGTTTATTGTGTCGAGAGAACCTTCCTTTTCCCTCTGTTTTTGCATCAAATTAAGTCATTGTGGTGTTTGTTTGTTTAAAGTCAGTCGTTTAAATTTCTCATTGTGAAAAACTAATTTTGTTTTACTTTGATGGGTTTGTGTATTTGCCTT
TARGET=1237,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1341
PRIMER_LEFT_PENALTY=0.018372
PRIMER_RIGHT_PENALTY=0.115769
PRIMER_LEFT_SEQUENCE=GAACCTTCCTTTTCCCTCTGTT
PRIMER_RIGHT_SEQUENCE=AAGGCAAATACACAAACCCATC
PRIMER_LEFT=1189,22
PRIMER_RIGHT=1322,22
PRIMER_LEFT_TM=59.982
PRIMER_RIGHT_TM=60.116
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.1368
PRIMER_LEFT_1_PENALTY=0.021044
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=CCTTCCTTTTCCCTCTGTTTTT
PRIMER_RIGHT_1_SEQUENCE=AAGGCAAATACACAAACCCATC
PRIMER_LEFT_1=1192,22
PRIMER_RIGHT_1=1322,22
PRIMER_LEFT_1_TM=59.979
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.1370
PRIMER_LEFT_2_PENALTY=0.021195
PRIMER_RIGHT_2_PENALTY=0.115769
PRIMER_LEFT_2_SEQUENCE=TATGGTCGTTTGAAGGCCTAGT
PRIMER_RIGHT_2_SEQUENCE=AAGGCAAATACACAAACCCATC
PRIMER_LEFT_2=1123,22
PRIMER_RIGHT_2=1322,22
PRIMER_LEFT_2_TM=60.021
PRIMER_RIGHT_2_TM=60.116
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=0.2247
PRIMER_LEFT_3_PENALTY=0.108929
PRIMER_RIGHT_3_PENALTY=0.115769
PRIMER_LEFT_3_SEQUENCE=CTTTTCCCTCTGTTTTTGCATC
PRIMER_RIGHT_3_SEQUENCE=AAGGCAAATACACAAACCCATC
PRIMER_LEFT_3=1197,22
PRIMER_RIGHT_3=1322,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=60.116
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.2518
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.115769
PRIMER_LEFT_4_SEQUENCE=TGTCGAGAGAACCTTCCTTTTC
PRIMER_RIGHT_4_SEQUENCE=AAGGCAAATACACAAACCCATC
PRIMER_LEFT_4=1181,22
PRIMER_RIGHT_4=1322,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=60.116
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=142
=
PRIMER_SEQUENCE_ID=SSR_00520 Ps_contig_mira-and-tgicl-ass_franssen_5195
SEQUENCE=TCTACTTTCTGGAAGTTTTTGACGCACGAAACGACGCGATCTGTGACCTCAGATTTATCGAGGAAAGAACCCCTAACCTCTTCGCTGAAGCGACGGAGGAAGAAATTGGGAAGAAGACGCAGAGGAGACTGGTGGTTGAGCGGAAGAGTATCAACCTGAACTCTTAGGTATTTCATCAAAGGCAAACCGGTTCTCCTCGCCGCCATTGCTGATATCAAAAACCTCACTCTCTCTCTCTCTCTCTGTTCAAATCTACACAACGAAGTGAAAAGAAATATTGTGAAGTTAGATCCMRTGATACCCAAACCAAACCTTGTTTTTAACACTGGGCTTTGTTTACTCC
TARGET=227,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00521 Ps_contig_mira-and-tgicl-ass_franssen_5196
SEQUENCE=GATCTAACTTCGACAATATTTCTTTTCACTTCGTTGTGTAGATTTGAACAGAGAGAGAGAGAGTGAGGTTTTTGATATCAGCAATGGCGGCGAAGGAGAACCGGTTT
TARGET=50,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00522 Ps_contig_mira-and-tgicl-ass_franssen_5197
SEQUENCE=CGGGGACAGAATSAGATCTAAAACTTTTGTTGTTGTTTTGTTTTTCTATATGTCTGATTTCCTATGTTTCTTCATTCATTCATGTTTCTTCAACTTGTCTGTCATTTCACCTTTCTTTCCTAAACCCCTAACTCTACACTCTCTATTTTCTCACACCNTGCACACCCAACCAATAACTACACCAACAAGAATCTTTGAGTACCTTTCCCAATACTAAATTTCAATCCGTTGGGTTTTTCTCATTCAACCATAAACG
TARGET=71,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00523 Ps_contig_mira-and-tgicl-ass_franssen_5199
SEQUENCE=CAAAGGTGTCTTCATCGGACTAAACTGAGGGAATATATCTGATCTTCGTCCTGGCTTAGGCCTCTCCCACTCGATTGGGAAATCGAGAGGCGAACGACGGACTAACTTCTCGTCTTCAGGAGCCATACGAACAACATGCCTCGAAAAACGCGAACGGGAAGAGATAGAGATGATAGACGGTAATCGATAGTTACAAGAAGAAGAAGAAGGAGGATTAGGTTTAAGCGAAGAAGAGAGAGGAGTGAGAATAGGGTTTAGGGTGAAGAAAGCCATTCTTGTTTTTCTTCAAGTCTTGACGCACCTAATCCTATCCCAACCTTTCGTTCCCTTTTCTTATGTTCCCCGAA
TARGET=195,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0322
PRIMER_LEFT_PENALTY=0.018835
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_RIGHT_SEQUENCE=GGATAGGATTAGGTGCGTCAAG
PRIMER_LEFT=102,22
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=0.0703
PRIMER_LEFT_1_PENALTY=0.056850
PRIMER_RIGHT_1_PENALTY=0.013401
PRIMER_LEFT_1_SEQUENCE=CGACGGACTAACTTCTCGTCTT
PRIMER_RIGHT_1_SEQUENCE=GGATAGGATTAGGTGCGTCAAG
PRIMER_LEFT_1=94,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=0.0750
PRIMER_LEFT_2_PENALTY=0.018835
PRIMER_RIGHT_2_PENALTY=0.056142
PRIMER_LEFT_2_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_RIGHT_2_SEQUENCE=GGGGAACATAAGAAAAGGGAAC
PRIMER_LEFT_2=102,22
PRIMER_RIGHT_2=343,22
PRIMER_LEFT_2_TM=60.019
PRIMER_RIGHT_2_TM=60.056
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.0946
PRIMER_LEFT_3_PENALTY=0.018835
PRIMER_RIGHT_3_PENALTY=0.075753
PRIMER_LEFT_3_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_RIGHT_3_SEQUENCE=AACGAAAGGTTGGGATAGGATT
PRIMER_LEFT_3=102,22
PRIMER_RIGHT_3=324,22
PRIMER_LEFT_3_TM=60.019
PRIMER_RIGHT_3_TM=60.076
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.1130
PRIMER_LEFT_4_PENALTY=0.056850
PRIMER_RIGHT_4_PENALTY=0.056142
PRIMER_LEFT_4_SEQUENCE=CGACGGACTAACTTCTCGTCTT
PRIMER_RIGHT_4_SEQUENCE=GGGGAACATAAGAAAAGGGAAC
PRIMER_LEFT_4=94,22
PRIMER_RIGHT_4=343,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=60.056
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=250
=
PRIMER_SEQUENCE_ID=SSR_00524 Ps_contig_mira-and-tgicl-ass_franssen_5200
SEQUENCE=GTCGGGGGGGTCGGGGTAGGTGCGTCAAGACTTGAAGAAAAACAAGAATGGCTTTCTTCACCCTAAACCCTATTCTCACTCCTCTCTCTTCTTCGCTTAAACCTAATCCTCCTTCTTCTTCTTGTAACTATCGATTACCGTCTATCATCTCTATCTCTTCCCGTTCGCGTTTTTCGAGGCATGTTGTTCGTATGGCTCCTGAAGACGAGAAGTTAGTCCGTCGTTCGCCTCTCGATTTCCCAATCGAGTGGGAGAAGGCCTAA
TARGET=112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1183
PRIMER_LEFT_PENALTY=0.099438
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=TCACTCCTCTCTCTTCTTCGCT
PRIMER_RIGHT_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_LEFT=75,22
PRIMER_RIGHT=214,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=140
PRIMER_PAIR_PENALTY_1=0.1183
PRIMER_LEFT_1_PENALTY=0.099438
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=CTCACTCCTCTCTCTTCTTCGC
PRIMER_RIGHT_1_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_LEFT_1=74,22
PRIMER_RIGHT_1=214,22
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.1563
PRIMER_LEFT_2_PENALTY=0.099438
PRIMER_RIGHT_2_PENALTY=0.056850
PRIMER_LEFT_2_SEQUENCE=TCACTCCTCTCTCTTCTTCGCT
PRIMER_RIGHT_2_SEQUENCE=CGACGGACTAACTTCTCGTCTT
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=222,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=0.1563
PRIMER_LEFT_3_PENALTY=0.099438
PRIMER_RIGHT_3_PENALTY=0.056850
PRIMER_LEFT_3_SEQUENCE=CTCACTCCTCTCTCTTCTTCGC
PRIMER_RIGHT_3_SEQUENCE=CGACGGACTAACTTCTCGTCTT
PRIMER_LEFT_3=74,22
PRIMER_RIGHT_3=222,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.2830
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.183585
PRIMER_LEFT_4_SEQUENCE=TCACTCCTCTCTCTTCTTCGCT
PRIMER_RIGHT_4_SEQUENCE=AACGACGGACTAACTTCTCGTC
PRIMER_LEFT_4=75,22
PRIMER_RIGHT_4=224,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=59.816
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_00525 Ps_contig_mira-and-tgicl-ass_franssen_5208
SEQUENCE=CTAATAAACCACACATTTATTATAGAAGATAACAACACAAACACAAACAACCTTGCCGTTGATGGAAAATTGTCTTCACCACCTTACAATCAAAGGATTCTTCTTCACAAAACCTATTTCTTTTTCATCTTCTTCTTCTTCTTCTTCTTCTTTGAAATTTAGGTGCAACAACAACAGCACCAAATTGAAGGTGATTAAGGCGAGTGCCGGAGCTAGCCACTGTCAGTTCAGTAGTTTGAACTCTCCTTTGTACCACAATCCRCGGTTGGTAAA
TARGET=128,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00526 Ps_contig_mira-and-tgicl-ass_franssen_5216
SEQUENCE=AATAATGGCTGTGGCTATGGCTGCTTGTAGCCTTGGACTTCACCTCACTCCTGATAATAATAATAATAATAATAATACTAATTGCTTTGCCCCTATCTTGAAAACCACTGCTATGTATGATACTAGTAAACCATGTTTCATTCTTCACAAAGCAAGATTATTATCTTCAGCAATTACGATCGTCCCTCGAGCAACAGCTTGTTTCTAATGTCGAGG
TARGET=54,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1336
PRIMER_LEFT_PENALTY=0.174518
PRIMER_RIGHT_PENALTY=0.959092
PRIMER_LEFT_SEQUENCE=GTAGCCTTGGACTTCACCTCAC
PRIMER_RIGHT_SEQUENCE=CCTCGACATTAGAAACAAGCTG
PRIMER_LEFT=26,22
PRIMER_RIGHT=215,22
PRIMER_LEFT_TM=60.175
PRIMER_RIGHT_TM=59.041
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=1.1486
PRIMER_LEFT_1_PENALTY=0.189529
PRIMER_RIGHT_1_PENALTY=0.959092
PRIMER_LEFT_1_SEQUENCE=CTTGTAGCCTTGGACTTCACCT
PRIMER_RIGHT_1_SEQUENCE=CCTCGACATTAGAAACAAGCTG
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=215,22
PRIMER_LEFT_1_TM=59.810
PRIMER_RIGHT_1_TM=59.041
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=1.2610
PRIMER_LEFT_2_PENALTY=0.301926
PRIMER_RIGHT_2_PENALTY=0.959092
PRIMER_LEFT_2_SEQUENCE=TAGCCTTGGACTTCACCTCACT
PRIMER_RIGHT_2_SEQUENCE=CCTCGACATTAGAAACAAGCTG
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=215,22
PRIMER_LEFT_2_TM=60.302
PRIMER_RIGHT_2_TM=59.041
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=1.4528
PRIMER_LEFT_3_PENALTY=0.174518
PRIMER_RIGHT_3_PENALTY=1.278288
PRIMER_LEFT_3_SEQUENCE=GTAGCCTTGGACTTCACCTCAC
PRIMER_RIGHT_3_SEQUENCE=GGACGATCGTAATTGCTGAAG
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=184,21
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=59.722
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=1.5802
PRIMER_LEFT_4_PENALTY=0.301926
PRIMER_RIGHT_4_PENALTY=1.278288
PRIMER_LEFT_4_SEQUENCE=TAGCCTTGGACTTCACCTCACT
PRIMER_RIGHT_4_SEQUENCE=GGACGATCGTAATTGCTGAAG
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=184,21
PRIMER_LEFT_4_TM=60.302
PRIMER_RIGHT_4_TM=59.722
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_00527 Ps_contig_mira-and-tgicl-ass_franssen_5217
SEQUENCE=TCGGGGATATAATCTCAACCAAACGCGWCACTCTTTTATTAGTTTTTCAAGATTAGAAGTGAACGTCACAGATTTATCTCCTATTTTCTAGATAAATTCACTCATTCATTCATTCTTCTCTCTCAAAAATCCCTTTATCCTCCTTCAACTCTTTCAATAATGGCTGTGGCTATGGCTGCTTGTAGCCTAGGACTTCACCTCACTCCTGATAATAATAATAATAATAATACTAATTGWCTTTCGCCCCTATCTTAGAAAACCACTGCTATGTATGATACTAACGTAAACC
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00528 Ps_contig_mira-and-tgicl-ass_franssen_5217
SEQUENCE=TCGGGGATATAATCTCAACCAAACGCGWCACTCTTTTATTAGTTTTTCAAGATTAGAAGTGAACGTCACAGATTTATCTCCTATTTTCTAGATAAATTCACTCATTCATTCATTCTTCTCTCTCAAAAATCCCTTTATCCTCCTTCAACTCTTTCAATAATGGCTGTGGCTATGGCTGCTTGTAGCCTAGGACTTCACCTCACTCCTGATAATAATAATAATAATAATACTAATTGWCTTTCGCCCCTATCTTAGAAAACCACTGCTATGTATGATACTAACGTAAACC
TARGET=209,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00529 Ps_contig_mira-and-tgicl-ass_franssen_5222
SEQUENCE=TTTTTTTGTAGGGAAGAGTGATCGTTTGCAGAGTTATTTGTAGCCATAGCTTCTGAGGCAGCGAAACAGAGTTTGAAAAAGAAGGGTTTGCATGTTCCACCATGGAGAAAACTCGAGTACGTTAGATCAAAATGGTTATCTCCTTACACAAGAACCAAACAACCTCTGTTTGCAATTGGCCTCGTGAAATCAGTGAGGAAACTGAGAAGAAAACAGGTAGGGGTTTTTGATTGGGTGGTGGTGGTGGTGAGTCTGAAGTGAGTCGNGA
TARGET=234,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00530 Ps_contig_mira-and-tgicl-ass_franssen_5223
SEQUENCE=AGGAAAACAAGAGGGTTTGGATTGGTGGTGGTGGTGGTGGAGGTCTGAAGTGAGTCGCGATGAGTCAGAGTCAAAAGCGGTGGTTGAATGGAAGCCACCGGAATTGAAACCCAAAAACTCGCTGACCGGGGTTAAGGTTGTGACTGGTTTGGCAGCTGTCTTTCGTGAAAACCCATGAAATTTTGTTTTTTAACCCAAAAAAAAAGAAGTCAGGTTTTTGTATTTTAATATGAGTATTTTAATTCTCTATATAGATAGAGAATTACAAGAGCTTGTGTAAGTACTGTAATAGGCTAACAGCTACTAACCCCTTTSTTAKTTTWWTAAAAGKTAAAAAAAAGG
TARGET=23,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00531 Ps_contig_mira-and-tgicl-ass_franssen_5245
SEQUENCE=CGTTTTTTTAGTAAGAGAGTTTCAAATTGCCTTTGAAGGTAAAAAACCTCACACCATACAATCCAAGTCTTTTCATTATATATACAAGCTAACAGCATGACATAATTTTATTTATGCACATTGAAGGAAATAGAATAAAGCATTCTACATAGTTTACTCATTGTGCTTCCGCGACAGAGTAATGCATTTCTGTAACTGATTTTTTCAAGAAACTAGCTCCGCAGTTTGAATCTTGGCTACAAACATCAACAGAGGCAAAATCAGAAGAACCTTCAATAACTTGAGAATCCGACAACTCATAGCACAGTTCAGAATGTTCACCAAGGTTGCGAGACTGTTTCTTATCTTTATCTTTGCCATTGCTCCCTCGAGTTGAATTTCCCTTGTTACCTTTCTTCAAACTTTTCTTTTCATTCTTCTTCTTCTTGGATTTCGGCTGTTTCTTGTTTTTGACAGTATGCGACACTAATGTAGGTATTGGATCATACACATCAGCAGCCC
TARGET=414,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0862
PRIMER_LEFT_PENALTY=0.023406
PRIMER_RIGHT_PENALTY=1.062826
PRIMER_LEFT_SEQUENCE=TTTTTCAAGAAACTAGCTCCGC
PRIMER_RIGHT_SEQUENCE=GCTGCTGATGTGTATGATCCAA
PRIMER_LEFT=200,22
PRIMER_RIGHT=498,22
PRIMER_LEFT_TM=60.023
PRIMER_RIGHT_TM=61.063
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=1.0960
PRIMER_LEFT_1_PENALTY=0.023406
PRIMER_RIGHT_1_PENALTY=1.072607
PRIMER_LEFT_1_SEQUENCE=TTTTTCAAGAAACTAGCTCCGC
PRIMER_RIGHT_1_SEQUENCE=CTGTCAAAAACAAGAAACAGCC
PRIMER_LEFT_1=200,22
PRIMER_RIGHT_1=455,22
PRIMER_LEFT_1_TM=60.023
PRIMER_RIGHT_1_TM=58.927
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=1.1142
PRIMER_LEFT_2_PENALTY=0.041553
PRIMER_RIGHT_2_PENALTY=1.072607
PRIMER_LEFT_2_SEQUENCE=CTAGCTCCGCAGTTTGAATCTT
PRIMER_RIGHT_2_SEQUENCE=CTGTCAAAAACAAGAAACAGCC
PRIMER_LEFT_2=212,22
PRIMER_RIGHT_2=455,22
PRIMER_LEFT_2_TM=60.042
PRIMER_RIGHT_2_TM=58.927
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=1.1535
PRIMER_LEFT_3_PENALTY=0.080887
PRIMER_RIGHT_3_PENALTY=1.072607
PRIMER_LEFT_3_SEQUENCE=AACTAGCTCCGCAGTTTGAATC
PRIMER_RIGHT_3_SEQUENCE=CTGTCAAAAACAAGAAACAGCC
PRIMER_LEFT_3=210,22
PRIMER_RIGHT_3=455,22
PRIMER_LEFT_3_TM=59.919
PRIMER_RIGHT_3_TM=58.927
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=1.2124
PRIMER_LEFT_4_PENALTY=0.139753
PRIMER_RIGHT_4_PENALTY=1.072607
PRIMER_LEFT_4_SEQUENCE=GAATCCGACAACTCATAGCACA
PRIMER_RIGHT_4_SEQUENCE=CTGTCAAAAACAAGAAACAGCC
PRIMER_LEFT_4=284,22
PRIMER_RIGHT_4=455,22
PRIMER_LEFT_4_TM=60.140
PRIMER_RIGHT_4_TM=58.927
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_00532 Ps_contig_mira-and-tgicl-ass_franssen_5250
SEQUENCE=GAATAATTGACCTGTTTTTTCTTAATTAGTGTGTTTAATGYAATTCATTTTAATTGGGGAAATGGTTGCTTGTGTATGCACTTTGAATTTTTTTTTTTTGGCTGAAGCACTTTCAGTTATGTATGATGCTCAAAAGTCCAAGAAATATATATATTTGTAGTTTTAAC
TARGET=145,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00533 Ps_contig_mira-and-tgicl-ass_franssen_5251
SEQUENCE=TATTCACTTTGTTGATATGACCGAATGTTGAAAGGATCATCTTGGACCTGAGRGTTCATCTGGCTGGATATGTGAAGTTGAATCGACTATCATCCAATTTGCATTAGGGTGATGCATGTGGTTGTTGTTAAAATCTTGTCGATACGTCTTTTCTTTCTGCCATTGCTCCCATTTTTCTGCCAAACCATCACCTTCTAGCATTCTCACCACCTCAGACATCTTTGGTCTTTCCATCGGCGACCCTTGTGTGCATAGTAAAGCCACTTGGATTAACTTCTCTACCTCATCATCATCATAATTTCCCGTTAATTCTGCATCTACAAGTGTTTCCAACTTCTTGTCTTTGAGAAGTCCTTTAACCCAAT
TARGET=284,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00534 Ps_contig_mira-and-tgicl-ass_franssen_5257
SEQUENCE=TAAAAAAAGGAATTAAAGATGAACCACTGTTCATCTTCCTAACAACAACCCGTAACCACCGCGAAACCCATCATGAAACCTCCGTTTCCTCCTTTTCACACCTCCTTCGCTCCGCCGCATCAACCTTCTTCAAACACACACATTACCGGCGAACCGACCCCTCTCCGAACACAACAACCATAACTCCATAAATTTTCGTCGCCGCCGCGAAAAACTTCTGCAACAAAATATCAACCGCGCATCACCGACTCCTTCTGCCGTGATTCACAACCGATGACTCCTTTTTCTCCACATCCTCCGTCTACGCGCATATCCAACACCTTGCATCCACTACAATCCCACACGGCCAAGATATCGTACCACCATCTCCGAACTCAATCTCCAAGGTGGTTTTCTTTTTAACTTTAAAATTTGAGTTGTTTTGTGATTTGTTTTGTTTATGTTTTAATTATTCTTCTTTGATTTGGAATTGGAGAGAGATTGGATGAAACAAAGTATGGATTTTGTTTTATTTCAAATGATCATTGTTGTTGTTGATTGCTGTTGAGAGAAAGGAGAGTGTGAGTTTTTTTATTCTTTTTTTTCCGTTTGAAATAAAAAGAAGAAGCTTTGTTCTTCTTTATTTTTCTGAAAGAAAGATCTGTTGCATTGGTGGTTTCATTTTAAAGATGATGTTGTTTCTCATTTATTAGTTTTATTTGTGAAATTTTAAAATAGTTGAAAAAATCTACTAGATTTCATTTTACTGTTTTTTATAAATAAGAGGAGCATATACGGTTTTCTTGAAGGAGGAGAAATGTGTGGAAATAGAGTTTGTTCATTTTAGATCTAGGTTTCTTTCTTTAATAATGGACAATAATATTATTTAGTAATGTTTGAAAAGGTCATAATCATGTTATTTACTGTTTTTGAAGAGAGATGGAAACATGGGAGAGAGCTTTGTGGGGAAGAAACTTTTCTTTAATTTCATGTTTAGAAGGGTGATTTGAGTGAAATAGAGGATGTTGGTTTCTTAGATTTTTGTTTCTTTCTCTAAATTGCCAAGTGATAATTTGGATATGGCTAATGTTTTTTTTGAGAAAAGTAATGCTAGGCCTTAGGGGCGTCGCCCCAATTGTATATATTAAATTCTTGCTCATTTATTTTATTTATCTATTTATTTATTTTACTCATTAAGGTTTAATTTTAAATTATGGTTAGTGTTAATTATTGGGTTGGATTCAATTTATTTTTTACACCCCATGGTCATGTATACACTCTTACTATTTCTTTGTACTATTTTCTYTCTTGATTCGACTAATAGTAAATTAATTGATTAAGTTTTTTT
TARGET=525,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00535 Ps_contig_mira-and-tgicl-ass_franssen_5257
SEQUENCE=TAAAAAAAGGAATTAAAGATGAACCACTGTTCATCTTCCTAACAACAACCCGTAACCACCGCGAAACCCATCATGAAACCTCCGTTTCCTCCTTTTCACACCTCCTTCGCTCCGCCGCATCAACCTTCTTCAAACACACACATTACCGGCGAACCGACCCCTCTCCGAACACAACAACCATAACTCCATAAATTTTCGTCGCCGCCGCGAAAAACTTCTGCAACAAAATATCAACCGCGCATCACCGACTCCTTCTGCCGTGATTCACAACCGATGACTCCTTTTTCTCCACATCCTCCGTCTACGCGCATATCCAACACCTTGCATCCACTACAATCCCACACGGCCAAGATATCGTACCACCATCTCCGAACTCAATCTCCAAGGTGGTTTTCTTTTTAACTTTAAAATTTGAGTTGTTTTGTGATTTGTTTTGTTTATGTTTTAATTATTCTTCTTTGATTTGGAATTGGAGAGAGATTGGATGAAACAAAGTATGGATTTTGTTTTATTTCAAATGATCATTGTTGTTGTTGATTGCTGTTGAGAGAAAGGAGAGTGTGAGTTTTTTTATTCTTTTTTTTCCGTTTGAAATAAAAAGAAGAAGCTTTGTTCTTCTTTATTTTTCTGAAAGAAAGATCTGTTGCATTGGTGGTTTCATTTTAAAGATGATGTTGTTTCTCATTTATTAGTTTTATTTGTGAAATTTTAAAATAGTTGAAAAAATCTACTAGATTTCATTTTACTGTTTTTTATAAATAAGAGGAGCATATACGGTTTTCTTGAAGGAGGAGAAATGTGTGGAAATAGAGTTTGTTCATTTTAGATCTAGGTTTCTTTCTTTAATAATGGACAATAATATTATTTAGTAATGTTTGAAAAGGTCATAATCATGTTATTTACTGTTTTTGAAGAGAGATGGAAACATGGGAGAGAGCTTTGTGGGGAAGAAACTTTTCTTTAATTTCATGTTTAGAAGGGTGATTTGAGTGAAATAGAGGATGTTGGTTTCTTAGATTTTTGTTTCTTTCTCTAAATTGCCAAGTGATAATTTGGATATGGCTAATGTTTTTTTTGAGAAAAGTAATGCTAGGCCTTAGGGGCGTCGCCCCAATTGTATATATTAAATTCTTGCTCATTTATTTTATTTATCTATTTATTTATTTTACTCATTAAGGTTTAATTTTAAATTATGGTTAGTGTTAATTATTGGGTTGGATTCAATTTATTTTTTACACCCCATGGTCATGTATACACTCTTACTATTTCTTTGTACTATTTTCTYTCTTGATTCGACTAATAGTAAATTAATTGATTAAGTTTTTTT
TARGET=1154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00536 Ps_contig_mira-and-tgicl-ass_franssen_5261
SEQUENCE=AGTGAAATAATATGGCTGAAGTTATTATATAGATCCTTCTAGTTAAAGCTGCGTGATTTTATAATCTTTAGTGATTAATTTGATTATTATATTGTTTAATAATTTATGTGTTTAATTAATTCTTTATAGCCTTTAGGCAATAATTGTATGGCTAATTATGAAGTTGTATTATTATTATTTATATAAAGGTGAATATGTACATGGGATGGGGAAACCATTTTTTAGTATATATACATCTATATATATTAGTCATCTTATAGGAAGGCTCAACCAAAGGTTTTGTAACCTTCCACTGTGTGTACAAGCAGTTTGTGGCTATTTAGTTTCATATGATAGATAAGTTTGTCCTTTCTGGCAATGATATTAATGATAGTGTTTTTTTTTAARTKTTA
TARGET=167,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00537 Ps_contig_mira-and-tgicl-ass_franssen_5262
SEQUENCE=AAACTAAAAAAAACACTATCATTAATATCATTGCCAGAAAGGACAAACTTATCTATCATATGAAACTAAATAGCCACAAACTGCTTGTACACACAGTGGAAGGTTACAAACCTTTGGTTGAGCCTTCCTATAAGATGACTAATATATATAGATGTATATATAACTAAAAATGTTCCCCACTCCCATGTACATATTCACCTTATATAAATAATAATAATACACTTCATAATTAG
TARGET=207,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00538 Ps_contig_mira-and-tgicl-ass_franssen_5263
SEQUENCE=CAGTGGTATCAACGCAGAGACGCGGGGAACAAGTAAAAAGATTAGAAATTTTCATGCAAAAACCACTAGACTTTCTTTTTATTTATTATTATTATTATGAGGTG
TARGET=83,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00539 Ps_contig_mira-and-tgicl-ass_franssen_5264
SEQUENCE=ATATAATAATCAAATTAATCACTAAAGATTTATAAAATCACACAGCTTTAACTAGAAGGATCTATATAACTTCAGCCATATTATTTCACTGTTTTCAAAGAATTCTTGTTATGAACTTGAGAATCCATTCTTGTGTCTTGAGATTGTTTTGGATATTTTGAGTATGAATTAGATCTTCCTTTCTTGTGCTTCCTTCTTGGTTGTGTTGCTTGTGGAATAAACACTCCTGTTCCTTTGCTTTCTCTTCTCACCAAAGCTTGCTCATAGAGGATATCAAACTGTGGAGGAGGATGAATTGCTCTAGAAAAAGCCTGAAAAATGAAACTTAACAATTATGTCGGTGCTATAGAATATTATATGCAAAAGGAAAAAAAATGTAGTTATGAGGTGTGTGAAGTAATTAAGGAGGTGTAATTAGTAAAATACCTGAAAAGAGTTTTGAGGTTGAGGAACAAAAGGATCTTCATCTTCATCCATAATTAAGAGAGCAATTTCCTTGCTCAAATTTGACAAAAAAAAAATCATCATCTAATTCAATAGCAACCTCCATAATAATAATAATAATAAAAAGAAAGTCTAGTGGTTTTGCATGAAAATTTCTAATCTTTTACTTGTTGTTCTCCMTAACTGGGGGATGTTTGGTTTTATATAGAGAAAAAGAACGGTAAGGA
TARGET=549,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00540 Ps_contig_mira-and-tgicl-ass_franssen_5274
SEQUENCE=AGCTCAAGGAATAAGTTGCTAGAGTCATCGCCCTGATGGAGTCACTCATTGCTGCTCATAACCAACCAGCTCCAACCCCTGCAACTCCTCAGCAAGGACTGTCATCTCTGAGATTATTGCAACACTAGTGTTTGTGTTTCCTGCCAGCCAGCCTGCTCAAGCTATGCATGCCAGATTCCCATGGGAAATGCCTCCTAATTTTATGCCTGAAGGTTATGCTCCAAATGTTGCTCCTATGCCGACATCTCACGGTCATGTCAATACCTCATCCTGTGGTGTATGTTATGCCACATGTTGATGAAACCATTTATCATTCTGAGCCGTATGAGGGCCTTGATGTGTATGAGAAAATGGATGAGATGAAAGACCAATTTCAAGAGTTAAGAAAATAATTGAAGACCTTGAGAGGTAAAAATTTGTTTGCAAAAAGTGCTGCTAAACTTTGCTTGGTGCCAAACGTGAAAATCCCTATCAAATTCAAGGTCCCAGATTTTGAGAAGTATAAAGGGAATACTTGTCCATTGAGCCATCTTGTGATGTATGCGCGCAAAATATCGACCCAGATAGATAGTGTTCAATTGCTCATCCATTATTTTCAAGACAATTTGACTGCTGTCATGCTTCAGCGGTATATGGGTTTAGACAGTGCTTGTGTCCATACTTTCAACGACTTAGGCGAGGCCTCCGTCAAGCAATACAAGTACAACATATATATAATGCCAGATCGTGATCAGTTGAGGTCCATGTCTCAGAAGGATAATGAGACATTCAAAGAGTATGCCCAGAGGTGGAGAGAGCTTGCCGCCTAGATTAGTCCCCCATTGGAGGAAAAGTAGATGACGAAGATCTTTTTGAAGACTTTGAGTTTGTTCTATTACGAGCGTATGATTTCCAGTGCTCCCAGTGACTTCACTAAAATGGTGAACATGGGGATTTGTTTAGAAGAGGGTGTCCGAGAGGGCCGTTTGTCTAAAGAAGAAGCATCTACGAGAAATAAGTATGGATGCAATTTCTCTAAGAAGAAGGAAGGGGAAACCAATTCAGTATCTGTGGGGAGGCAAAAGAGGCCTCATGTGAGGAGAAATTCCAGATCTCAGTATCATCATCATCATCAAGTATCCTATGCCATTCCAGCATTCACCAATAATTTCAATCATCTCCAGTTCAACATCAACAACAATGTACTCACAACAATCACAACAATTACAACAATCATCATAACAATTTTGAAAGGAAGAAAGTCACTTTTGATCCTATTCTGAGTCGTATGCAGAATTGTATCCTTCTCTCATCGTCAAGAATTTGATCCAGCTAAGGAATCCTCCGCATACACATGAGCCTCTGCCATGGTGGTCAAGCCCGATCTGCATTATGCTTTATATCAAGGGGCACCCGACCATGACATTGAGAATTGTTATCCTTTGAAGTATGAAGTTAAAAAATTGGTCAAAAGTGGGATGGTGTCCTTTGAGGACCGCGCACCTAATGTCAAAGCTAATCCTTTGCCCTCCCATGCAATTCTTTTATGAATATGGTAGATGGTTGTCTTGGTAATTTCAGAGTTTTTGATGTTCGTCGTATCTGAAGGTCTTTGGTGGAAATACATAGAACTTTGTGCATGATCAGTGATTGTGAACATGACCATGACAATTGTGTTATATACAATGTTAATCCCCGAGAATGTGCGATTGTGAAGAGGGACGTTTAGAAGTTGATGGGTGAAGGTGTTATTCAGATTAATCAGTCCAGAAATTTGGGTAATGATGTGAATGTAATTGTGCCAGTATTCAAGACTCCCGAGCGAGTAGTAATCCAGTTTGATAGCAGCAAAAGAGATAATAGATCGATATCGCTGTTGGTAATACGGTTAGCGGGCCCTATCCCTTATGCATCCGATAAAGTTGTACCTTACAAATATAATGCTACTATTATTGAGAATGGGCAAGAGGTTCCTCTTCCCGCAACAAATTCAGTAGTGAGCATTGCTGATACTGTGAAAGTGACCCATATCGGTCATGTATTTGGTTTAGTACCCATGAAGGTTATGGAGAATGTTGTGGTTGGTAAGAAGGCGAATGTTCCAGTGGTTAATCCTATTAACCCTCCAACTTTCCAGTCTGGTGAATCCAGCGGGTTGAAGGTTAAGGATGATGATGATGAGATTCTCTAGTTGATTAAGAAGAGTGAGTTCAATGTTATGGAGTAGATGCTCCAAACACCATCCAAGATCTTTATGATTTTATTGATGAATTATGAAGCGCACCGAGAGGCGTTGCAGAAGGTGCTTGAGAAAACCTATGTGGAACAGGAATTTACGGTTGATCAGTTTGAACATATCGTTGCCAATATTACTTCATGCAATAATTTGAGCTTTTGTGATCAATATATTAAAAAAAAAATTAAAAAATCAATCTAAAGGAACAAATCAATCAAATTTTATATCTACACCTTTAAATTAATCAATTTTAAAATACTCCTCTGCTTTCCATAATATGTCACTTTCATACATATTAAAAAATTATTTATTAACATGAAAAGGAAAAATGATGCATTATTTTATTAAATAATTGTATTAATAGTATAGGGAAAATAAAGTAATGAGCCGAAAGAAAAGATAGTAACTAATATTTAATAAAAGAGGTTCTAGTAGTACATRRSKKAAGAATTGTATTAAAAAATGAAAACCACATGCTCTTATTCTTTGGATTTGTAATCCTTATCTTCAAAATGGAATATTATTTTGTCTATATATTCAGTTTGATCAAGTATACAAAGATCAKATAGTGAGCAAACCAGTGCTAGCTAACTTATCAGTATGGCTATCCAAGAAACTTTGATAGACAAGCCTAGAAAATCCATTCCCAAAACTTTCTGGTTAATCCTCTCTTTAGCTGCTATCATAGGCTCATCAGCCCTTATTGTTTCTCATCTCAACAAACCTATCTCCTTCTTCCCACTCTCTTCAGCTCCCAATCTGTGTGAGCATGCTGTTGATACAAAATCATGCTTAACTCATGTATCAGAAGTGGTTCAAGGCCAAGCCTTAGCTAACACAAAAGACCACAAATTGAGTACACTCATATCCTTATTAACCAAGTCCACCTCACACATTCAGAAAGCCATGGAAACAGCCAATGTTATCAAACGCCGGGTTAACAGCCCTAGAGAGGAGACGGCTTTGAATGACTGTGAGCAACTAATGGACTTGTCCATGGATAGAGTTTGGGACTCAGTGTTGACTTTAACAAAAAATAACATTGACTCACAACAAGATGCACACACATGGCTAAGTAGTGTGCTCACTAACCATGCAACTTGTTTGAATGGTTTAGAAGGTACATCTCGGGTCGTCATGGAAAGTGACCTTCAGGACTTGATATCAAGAGCTAGATCTTCTCTCGCCGTGCTTGTTTCCGTTTTACCTGCAAAGAGTAACGACGGATTCATTGATGAATCATTGAACGGTGAATTTCCCTCATGGGTAACGAGTAAGGATCGAAGGCTTTTGGAGTCTACAGTTGGGGACATAAAGGCCAATGTTGTGGTGGCTAAAGATGGGAGTGGTAAGTTCAAAACTGTGGCTGAGGCTGTGGCATCTGCACCAGACAACGGTAAGGCAAGGTATGTTATCTATGTGAAAAGAGGAACCTACAAAGAGAAGGTAGAAATTGGTAAGAAAAAGACAAATGTGATGCTCGTTGGTGATGGTATGGATGCAACAATAATCACAGGCAACTTGAATTTTATCGATGGAACAACCACTTTCAATAGTGCAACTGTTGCTGCTGTTGGAGATGGGTTTATAGCTCAGGACATAGGGTTCCAAAACACGGCAGGCCCAGAAAAGCACCAGGCAGTTGCTCTCCGCGTAGGTGCTGATCAATCTGTCATCAACCGTTGTAAAATTGACGCATTTCAAGACACCCTCTACGCACACTCCAACCGACAATTTTACCGTGACTCCTTCATTACCGGTACTGTTGACTTTATCTTTGGAAACGCAGGTGTTGTGTTCCAGAAGAGCAAACTTGTGGCCCGAAAGCCCATGAGCAACCAAAAGAACATGGTCACGGCCCAAGGTCGAGAAGACCCAAACCAGAACACTGCAACTTCAATTCAGCAATGTAATGTCATACCAAGCTCGGACCTCAAGCCTGTGCAAGGCTCCATCAAAACATACCTAGGCCGCCCATGGAAGAAATACTCCAGGACTGTTGTGTTGCAGTCCGTCGTAGACAGCCATATTGACCCAGCAGGATGGGCTGAATGGGATGCCGCGAGTAAGGATTTTCTGCAAACATTGTATTACGGAGAGTACTTGAACAGTGGAGCAGGTGCTGGTACCAGCAAGAGAGTGACTTGGCCTGGTTATCATATCATCAAAACTGCTGCAGAGGCTAGCAAGTTTACAGTGACACAGCTCATCCAGGGTAATGTTTGGTTGAAGAACACAGGGGTAGCCTTCATTGAAGGCCTGTAGAAATTGGCTTCGGCAGGCGTGTACTATTATGTTTTTGATATGAGTGAAATTTGCAGGAAATAAAACAGAAACATTATCCTTTATGGAAGAATGAGGTTTAATTAAAAAAATTTCCTACATACGATGTAACAACTGTCTTCTACCAATGTTAAAATGAATGCCACATGTCTGGAAAATAAACTTTAGTAGTTAACARCC
TARGET=1099,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00541 Ps_contig_mira-and-tgicl-ass_franssen_5274
SEQUENCE=AGCTCAAGGAATAAGTTGCTAGAGTCATCGCCCTGATGGAGTCACTCATTGCTGCTCATAACCAACCAGCTCCAACCCCTGCAACTCCTCAGCAAGGACTGTCATCTCTGAGATTATTGCAACACTAGTGTTTGTGTTTCCTGCCAGCCAGCCTGCTCAAGCTATGCATGCCAGATTCCCATGGGAAATGCCTCCTAATTTTATGCCTGAAGGTTATGCTCCAAATGTTGCTCCTATGCCGACATCTCACGGTCATGTCAATACCTCATCCTGTGGTGTATGTTATGCCACATGTTGATGAAACCATTTATCATTCTGAGCCGTATGAGGGCCTTGATGTGTATGAGAAAATGGATGAGATGAAAGACCAATTTCAAGAGTTAAGAAAATAATTGAAGACCTTGAGAGGTAAAAATTTGTTTGCAAAAAGTGCTGCTAAACTTTGCTTGGTGCCAAACGTGAAAATCCCTATCAAATTCAAGGTCCCAGATTTTGAGAAGTATAAAGGGAATACTTGTCCATTGAGCCATCTTGTGATGTATGCGCGCAAAATATCGACCCAGATAGATAGTGTTCAATTGCTCATCCATTATTTTCAAGACAATTTGACTGCTGTCATGCTTCAGCGGTATATGGGTTTAGACAGTGCTTGTGTCCATACTTTCAACGACTTAGGCGAGGCCTCCGTCAAGCAATACAAGTACAACATATATATAATGCCAGATCGTGATCAGTTGAGGTCCATGTCTCAGAAGGATAATGAGACATTCAAAGAGTATGCCCAGAGGTGGAGAGAGCTTGCCGCCTAGATTAGTCCCCCATTGGAGGAAAAGTAGATGACGAAGATCTTTTTGAAGACTTTGAGTTTGTTCTATTACGAGCGTATGATTTCCAGTGCTCCCAGTGACTTCACTAAAATGGTGAACATGGGGATTTGTTTAGAAGAGGGTGTCCGAGAGGGCCGTTTGTCTAAAGAAGAAGCATCTACGAGAAATAAGTATGGATGCAATTTCTCTAAGAAGAAGGAAGGGGAAACCAATTCAGTATCTGTGGGGAGGCAAAAGAGGCCTCATGTGAGGAGAAATTCCAGATCTCAGTATCATCATCATCATCAAGTATCCTATGCCATTCCAGCATTCACCAATAATTTCAATCATCTCCAGTTCAACATCAACAACAATGTACTCACAACAATCACAACAATTACAACAATCATCATAACAATTTTGAAAGGAAGAAAGTCACTTTTGATCCTATTCTGAGTCGTATGCAGAATTGTATCCTTCTCTCATCGTCAAGAATTTGATCCAGCTAAGGAATCCTCCGCATACACATGAGCCTCTGCCATGGTGGTCAAGCCCGATCTGCATTATGCTTTATATCAAGGGGCACCCGACCATGACATTGAGAATTGTTATCCTTTGAAGTATGAAGTTAAAAAATTGGTCAAAAGTGGGATGGTGTCCTTTGAGGACCGCGCACCTAATGTCAAAGCTAATCCTTTGCCCTCCCATGCAATTCTTTTATGAATATGGTAGATGGTTGTCTTGGTAATTTCAGAGTTTTTGATGTTCGTCGTATCTGAAGGTCTTTGGTGGAAATACATAGAACTTTGTGCATGATCAGTGATTGTGAACATGACCATGACAATTGTGTTATATACAATGTTAATCCCCGAGAATGTGCGATTGTGAAGAGGGACGTTTAGAAGTTGATGGGTGAAGGTGTTATTCAGATTAATCAGTCCAGAAATTTGGGTAATGATGTGAATGTAATTGTGCCAGTATTCAAGACTCCCGAGCGAGTAGTAATCCAGTTTGATAGCAGCAAAAGAGATAATAGATCGATATCGCTGTTGGTAATACGGTTAGCGGGCCCTATCCCTTATGCATCCGATAAAGTTGTACCTTACAAATATAATGCTACTATTATTGAGAATGGGCAAGAGGTTCCTCTTCCCGCAACAAATTCAGTAGTGAGCATTGCTGATACTGTGAAAGTGACCCATATCGGTCATGTATTTGGTTTAGTACCCATGAAGGTTATGGAGAATGTTGTGGTTGGTAAGAAGGCGAATGTTCCAGTGGTTAATCCTATTAACCCTCCAACTTTCCAGTCTGGTGAATCCAGCGGGTTGAAGGTTAAGGATGATGATGATGAGATTCTCTAGTTGATTAAGAAGAGTGAGTTCAATGTTATGGAGTAGATGCTCCAAACACCATCCAAGATCTTTATGATTTTATTGATGAATTATGAAGCGCACCGAGAGGCGTTGCAGAAGGTGCTTGAGAAAACCTATGTGGAACAGGAATTTACGGTTGATCAGTTTGAACATATCGTTGCCAATATTACTTCATGCAATAATTTGAGCTTTTGTGATCAATATATTAAAAAAAAAATTAAAAAATCAATCTAAAGGAACAAATCAATCAAATTTTATATCTACACCTTTAAATTAATCAATTTTAAAATACTCCTCTGCTTTCCATAATATGTCACTTTCATACATATTAAAAAATTATTTATTAACATGAAAAGGAAAAATGATGCATTATTTTATTAAATAATTGTATTAATAGTATAGGGAAAATAAAGTAATGAGCCGAAAGAAAAGATAGTAACTAATATTTAATAAAAGAGGTTCTAGTAGTACATRRSKKAAGAATTGTATTAAAAAATGAAAACCACATGCTCTTATTCTTTGGATTTGTAATCCTTATCTTCAAAATGGAATATTATTTTGTCTATATATTCAGTTTGATCAAGTATACAAAGATCAKATAGTGAGCAAACCAGTGCTAGCTAACTTATCAGTATGGCTATCCAAGAAACTTTGATAGACAAGCCTAGAAAATCCATTCCCAAAACTTTCTGGTTAATCCTCTCTTTAGCTGCTATCATAGGCTCATCAGCCCTTATTGTTTCTCATCTCAACAAACCTATCTCCTTCTTCCCACTCTCTTCAGCTCCCAATCTGTGTGAGCATGCTGTTGATACAAAATCATGCTTAACTCATGTATCAGAAGTGGTTCAAGGCCAAGCCTTAGCTAACACAAAAGACCACAAATTGAGTACACTCATATCCTTATTAACCAAGTCCACCTCACACATTCAGAAAGCCATGGAAACAGCCAATGTTATCAAACGCCGGGTTAACAGCCCTAGAGAGGAGACGGCTTTGAATGACTGTGAGCAACTAATGGACTTGTCCATGGATAGAGTTTGGGACTCAGTGTTGACTTTAACAAAAAATAACATTGACTCACAACAAGATGCACACACATGGCTAAGTAGTGTGCTCACTAACCATGCAACTTGTTTGAATGGTTTAGAAGGTACATCTCGGGTCGTCATGGAAAGTGACCTTCAGGACTTGATATCAAGAGCTAGATCTTCTCTCGCCGTGCTTGTTTCCGTTTTACCTGCAAAGAGTAACGACGGATTCATTGATGAATCATTGAACGGTGAATTTCCCTCATGGGTAACGAGTAAGGATCGAAGGCTTTTGGAGTCTACAGTTGGGGACATAAAGGCCAATGTTGTGGTGGCTAAAGATGGGAGTGGTAAGTTCAAAACTGTGGCTGAGGCTGTGGCATCTGCACCAGACAACGGTAAGGCAAGGTATGTTATCTATGTGAAAAGAGGAACCTACAAAGAGAAGGTAGAAATTGGTAAGAAAAAGACAAATGTGATGCTCGTTGGTGATGGTATGGATGCAACAATAATCACAGGCAACTTGAATTTTATCGATGGAACAACCACTTTCAATAGTGCAACTGTTGCTGCTGTTGGAGATGGGTTTATAGCTCAGGACATAGGGTTCCAAAACACGGCAGGCCCAGAAAAGCACCAGGCAGTTGCTCTCCGCGTAGGTGCTGATCAATCTGTCATCAACCGTTGTAAAATTGACGCATTTCAAGACACCCTCTACGCACACTCCAACCGACAATTTTACCGTGACTCCTTCATTACCGGTACTGTTGACTTTATCTTTGGAAACGCAGGTGTTGTGTTCCAGAAGAGCAAACTTGTGGCCCGAAAGCCCATGAGCAACCAAAAGAACATGGTCACGGCCCAAGGTCGAGAAGACCCAAACCAGAACACTGCAACTTCAATTCAGCAATGTAATGTCATACCAAGCTCGGACCTCAAGCCTGTGCAAGGCTCCATCAAAACATACCTAGGCCGCCCATGGAAGAAATACTCCAGGACTGTTGTGTTGCAGTCCGTCGTAGACAGCCATATTGACCCAGCAGGATGGGCTGAATGGGATGCCGCGAGTAAGGATTTTCTGCAAACATTGTATTACGGAGAGTACTTGAACAGTGGAGCAGGTGCTGGTACCAGCAAGAGAGTGACTTGGCCTGGTTATCATATCATCAAAACTGCTGCAGAGGCTAGCAAGTTTACAGTGACACAGCTCATCCAGGGTAATGTTTGGTTGAAGAACACAGGGGTAGCCTTCATTGAAGGCCTGTAGAAATTGGCTTCGGCAGGCGTGTACTATTATGTTTTTGATATGAGTGAAATTTGCAGGAAATAAAACAGAAACATTATCCTTTATGGAAGAATGAGGTTTAATTAAAAAAATTTCCTACATACGATGTAACAACTGTCTTCTACCAATGTTAAAATGAATGCCACATGTCTGGAAAATAAACTTTAGTAGTTAACARCC
TARGET=2147,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00542 Ps_contig_mira-and-tgicl-ass_franssen_5275
SEQUENCE=CTCAAAATACACAACACCACAAGCATACGAAATTGATATATTTACCGAAGCTGTGTCACTGTTGTCGTTTGTTGTTGCTGCGAAGCTGGAAGGTTGGTTGCTTGGTTTCGCCCGTTGGGGAAGAGAGAATCTGCGTCGCGGTTGGTCGTGGTGAATTTAGAAGAGCGAACCGCGGCGGCGGCGATGTTGGTGAACAGAGGTGGATTCGCCTGAGTGTGAAGGAGCTTGGTTATTGACGCGACGGTGAGGTTTGGAATTTGTCGCCGGAATAGATGAGGAAGACGGCAGATGGTTCAAAATTGACGGTGGTTGAGCGATGATGCGGTGACGTAGTGGTAGTGGTGGCGATTCGACGGAGGTTAGTCTAGGTTTGTTTTGGCGGTTTAGGTAAAAGGGGGTTTTGTTGGTTTGTTGKTWAAAGGGTGTGTCGGTGAGGTTGTTGCGAGACGGAGGAAGACGATGGGTGAGTGGAAGCTATGGCCTCATAGTGGTAGTGGTGGCGATTCACGGTGGAGGATTTTTG
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00543 Ps_contig_mira-and-tgicl-ass_franssen_5278
SEQUENCE=GATAATGGTGATCTGAAAATCTTTTATTTGAGAGATATCCAAAGCTAATCTCGTTGCGAAGGTGGGCGTGGTCATCAAAGCTAATTTGGTTATGGTCATCATGTATCCGTTATTGCAGCATCATGTTATGTTTAGGAATACTTGTTGCATGAAGCAAAATCAACAGCGGCAGTATGCATCGTTGAGAAGCAATGAAAAGCAGCAGCAGCGGCAGTATGCATCGGCGGCGGCGAGAGACAGAGTAATAGATTTCGGTAAATATAAGGGGAAGATGCTGGGAAGCCTTCCATCAAGCTATCTGAAATGGGTTTCAAAAAACTTACGTGCACGGGATTTTGAGAAATGGGCAATTCTTGCCGATCAAGTCCTTGTGGACCCAATCTACAAAGACCGCATCGAGTGGGAGTTTGCTCTCAATGTGTTAGAAAGAAACAGATCCGGTGAGCCCGCAGTTTCTCAACTACAGGACATAAGCGACAGGTTTGGTTGGGATTATCTTGACAAACTTGGTTGGAGCAAAGTTGATTTCGACCTTCTCGGCACTTCTAATGGAGGGAGAATTCCGAGACTAGACAATCCAAGGGACGCCGACGCCAGTCAACCCAGTCTCCCACCTGCTACTACTACTAGTAACAATAGACCCAATCTCCGACCTGCTACTACTAGTAGGAGGATGGAGAGAAGAGAGAAACTGAAAATGAGATCGAGGACGACACATGATGAAGAAGAAGAAGAAGCGCAGCAGCAGCACAAGGACAAGGACGATACTGTGCCTGCTGCTGATATTAAACAAATTGCCAACCCATTCCCTGGACGTCAGGCTCTCCTCCGAAAAGCCATCAACTATCGCGCACAATCCTAAACAACTTTCTATCATCCATCTACTCTAATTAACTAATTTCCTTTTTTACTTTTTTCTTTTAATTAAGATTCTTGTATGTTATTACCCG
TARGET=198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2474
PRIMER_LEFT_PENALTY=0.089133
PRIMER_RIGHT_PENALTY=0.158297
PRIMER_LEFT_SEQUENCE=AGTATGCATCGTTGAGAAGCAA
PRIMER_RIGHT_SEQUENCE=ACTCGATGCGGTCTTTGTAGAT
PRIMER_LEFT=170,22
PRIMER_RIGHT=400,22
PRIMER_LEFT_TM=59.911
PRIMER_RIGHT_TM=60.158
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.3542
PRIMER_LEFT_1_PENALTY=0.089133
PRIMER_RIGHT_1_PENALTY=0.265053
PRIMER_LEFT_1_SEQUENCE=AGTATGCATCGTTGAGAAGCAA
PRIMER_RIGHT_1_SEQUENCE=GGTCTTTGTAGATTGGGTCCAC
PRIMER_LEFT_1=170,22
PRIMER_RIGHT_1=391,22
PRIMER_LEFT_1_TM=59.911
PRIMER_RIGHT_1_TM=59.735
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.3843
PRIMER_LEFT_2_PENALTY=0.119274
PRIMER_RIGHT_2_PENALTY=0.265053
PRIMER_LEFT_2_SEQUENCE=CATGTATCCGTTATTGCAGCAT
PRIMER_RIGHT_2_SEQUENCE=GGTCTTTGTAGATTGGGTCCAC
PRIMER_LEFT_2=99,22
PRIMER_RIGHT_2=391,22
PRIMER_LEFT_2_TM=59.881
PRIMER_RIGHT_2_TM=59.735
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.4495
PRIMER_LEFT_3_PENALTY=0.089133
PRIMER_RIGHT_3_PENALTY=0.360320
PRIMER_LEFT_3_SEQUENCE=AGTATGCATCGTTGAGAAGCAA
PRIMER_RIGHT_3_SEQUENCE=TCAGATAGCTTGATGGAAGGCT
PRIMER_LEFT_3=170,22
PRIMER_RIGHT_3=301,22
PRIMER_LEFT_3_TM=59.911
PRIMER_RIGHT_3_TM=60.360
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=0.4796
PRIMER_LEFT_4_PENALTY=0.119274
PRIMER_RIGHT_4_PENALTY=0.360320
PRIMER_LEFT_4_SEQUENCE=CATGTATCCGTTATTGCAGCAT
PRIMER_RIGHT_4_SEQUENCE=TCAGATAGCTTGATGGAAGGCT
PRIMER_LEFT_4=99,22
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=59.881
PRIMER_RIGHT_4_TM=60.360
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_00544 Ps_contig_mira-and-tgicl-ass_franssen_5278
SEQUENCE=GATAATGGTGATCTGAAAATCTTTTATTTGAGAGATATCCAAAGCTAATCTCGTTGCGAAGGTGGGCGTGGTCATCAAAGCTAATTTGGTTATGGTCATCATGTATCCGTTATTGCAGCATCATGTTATGTTTAGGAATACTTGTTGCATGAAGCAAAATCAACAGCGGCAGTATGCATCGTTGAGAAGCAATGAAAAGCAGCAGCAGCGGCAGTATGCATCGGCGGCGGCGAGAGACAGAGTAATAGATTTCGGTAAATATAAGGGGAAGATGCTGGGAAGCCTTCCATCAAGCTATCTGAAATGGGTTTCAAAAAACTTACGTGCACGGGATTTTGAGAAATGGGCAATTCTTGCCGATCAAGTCCTTGTGGACCCAATCTACAAAGACCGCATCGAGTGGGAGTTTGCTCTCAATGTGTTAGAAAGAAACAGATCCGGTGAGCCCGCAGTTTCTCAACTACAGGACATAAGCGACAGGTTTGGTTGGGATTATCTTGACAAACTTGGTTGGAGCAAAGTTGATTTCGACCTTCTCGGCACTTCTAATGGAGGGAGAATTCCGAGACTAGACAATCCAAGGGACGCCGACGCCAGTCAACCCAGTCTCCCACCTGCTACTACTACTAGTAACAATAGACCCAATCTCCGACCTGCTACTACTAGTAGGAGGATGGAGAGAAGAGAGAAACTGAAAATGAGATCGAGGACGACACATGATGAAGAAGAAGAAGAAGCGCAGCAGCAGCACAAGGACAAGGACGATACTGTGCCTGCTGCTGATATTAAACAAATTGCCAACCCATTCCCTGGACGTCAGGCTCTCCTCCGAAAAGCCATCAACTATCGCGCACAATCCTAAACAACTTTCTATCATCCATCTACTCTAATTAACTAATTTCCTTTTTTACTTTTTTCTTTTAATTAAGATTCTTGTATGTTATTACCCG
TARGET=618,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1733
PRIMER_LEFT_PENALTY=0.054514
PRIMER_RIGHT_PENALTY=0.118777
PRIMER_LEFT_SEQUENCE=GGTTTGGTTGGGATTATCTTGA
PRIMER_RIGHT_SEQUENCE=TTCTTCATCATGTGTCGTCCTC
PRIMER_LEFT=479,22
PRIMER_RIGHT=726,22
PRIMER_LEFT_TM=60.055
PRIMER_RIGHT_TM=60.119
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.1733
PRIMER_LEFT_1_PENALTY=0.054514
PRIMER_RIGHT_1_PENALTY=0.118777
PRIMER_LEFT_1_SEQUENCE=GGTTTGGTTGGGATTATCTTGA
PRIMER_RIGHT_1_SEQUENCE=TCTTCTTCATCATGTGTCGTCC
PRIMER_LEFT_1=479,22
PRIMER_RIGHT_1=728,22
PRIMER_LEFT_1_TM=60.055
PRIMER_RIGHT_1_TM=60.119
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.1796
PRIMER_LEFT_2_PENALTY=0.060824
PRIMER_RIGHT_2_PENALTY=0.118777
PRIMER_LEFT_2_SEQUENCE=ACAGGACATAAGCGACAGGTTT
PRIMER_RIGHT_2_SEQUENCE=TTCTTCATCATGTGTCGTCCTC
PRIMER_LEFT_2=462,22
PRIMER_RIGHT_2=726,22
PRIMER_LEFT_2_TM=60.061
PRIMER_RIGHT_2_TM=60.119
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.1796
PRIMER_LEFT_3_PENALTY=0.060824
PRIMER_RIGHT_3_PENALTY=0.118777
PRIMER_LEFT_3_SEQUENCE=ACAGGACATAAGCGACAGGTTT
PRIMER_RIGHT_3_SEQUENCE=TCTTCTTCATCATGTGTCGTCC
PRIMER_LEFT_3=462,22
PRIMER_RIGHT_3=728,22
PRIMER_LEFT_3_TM=60.061
PRIMER_RIGHT_3_TM=60.119
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.2056
PRIMER_LEFT_4_PENALTY=0.054514
PRIMER_RIGHT_4_PENALTY=0.151106
PRIMER_LEFT_4_SEQUENCE=GGTTTGGTTGGGATTATCTTGA
PRIMER_RIGHT_4_SEQUENCE=GTAGTAGCAGGTCGGAGATTGG
PRIMER_LEFT_4=479,22
PRIMER_RIGHT_4=662,22
PRIMER_LEFT_4_TM=60.055
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_00545 Ps_contig_mira-and-tgicl-ass_franssen_5278
SEQUENCE=GATAATGGTGATCTGAAAATCTTTTATTTGAGAGATATCCAAAGCTAATCTCGTTGCGAAGGTGGGCGTGGTCATCAAAGCTAATTTGGTTATGGTCATCATGTATCCGTTATTGCAGCATCATGTTATGTTTAGGAATACTTGTTGCATGAAGCAAAATCAACAGCGGCAGTATGCATCGTTGAGAAGCAATGAAAAGCAGCAGCAGCGGCAGTATGCATCGGCGGCGGCGAGAGACAGAGTAATAGATTTCGGTAAATATAAGGGGAAGATGCTGGGAAGCCTTCCATCAAGCTATCTGAAATGGGTTTCAAAAAACTTACGTGCACGGGATTTTGAGAAATGGGCAATTCTTGCCGATCAAGTCCTTGTGGACCCAATCTACAAAGACCGCATCGAGTGGGAGTTTGCTCTCAATGTGTTAGAAAGAAACAGATCCGGTGAGCCCGCAGTTTCTCAACTACAGGACATAAGCGACAGGTTTGGTTGGGATTATCTTGACAAACTTGGTTGGAGCAAAGTTGATTTCGACCTTCTCGGCACTTCTAATGGAGGGAGAATTCCGAGACTAGACAATCCAAGGGACGCCGACGCCAGTCAACCCAGTCTCCCACCTGCTACTACTACTAGTAACAATAGACCCAATCTCCGACCTGCTACTACTAGTAGGAGGATGGAGAGAAGAGAGAAACTGAAAATGAGATCGAGGACGACACATGATGAAGAAGAAGAAGAAGCGCAGCAGCAGCACAAGGACAAGGACGATACTGTGCCTGCTGCTGATATTAAACAAATTGCCAACCCATTCCCTGGACGTCAGGCTCTCCTCCGAAAAGCCATCAACTATCGCGCACAATCCTAAACAACTTTCTATCATCCATCTACTCTAATTAACTAATTTCCTTTTTTACTTTTTTCTTTTAATTAAGATTCTTGTATGTTATTACCCG
TARGET=722,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2185
PRIMER_LEFT_PENALTY=0.151106
PRIMER_RIGHT_PENALTY=0.067430
PRIMER_LEFT_SEQUENCE=CCAATCTCCGACCTGCTACTAC
PRIMER_RIGHT_SEQUENCE=AATATCAGCAGCAGGCACAGTA
PRIMER_LEFT=641,22
PRIMER_RIGHT=786,22
PRIMER_LEFT_TM=60.151
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.1000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=0.2185
PRIMER_LEFT_1_PENALTY=0.151106
PRIMER_RIGHT_1_PENALTY=0.067430
PRIMER_LEFT_1_SEQUENCE=CCAATCTCCGACCTGCTACTAC
PRIMER_RIGHT_1_SEQUENCE=TAATATCAGCAGCAGGCACAGT
PRIMER_LEFT_1=641,22
PRIMER_RIGHT_1=787,22
PRIMER_LEFT_1_TM=60.151
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.1000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.2557
PRIMER_LEFT_2_PENALTY=0.188254
PRIMER_RIGHT_2_PENALTY=0.067430
PRIMER_LEFT_2_SEQUENCE=CCAGTCTCCCACCTGCTACTAC
PRIMER_RIGHT_2_SEQUENCE=AATATCAGCAGCAGGCACAGTA
PRIMER_LEFT_2=602,22
PRIMER_RIGHT_2=786,22
PRIMER_LEFT_2_TM=60.188
PRIMER_RIGHT_2_TM=59.933
PRIMER_LEFT_2_GC_PERCENT=59.091
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.1000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.2837
PRIMER_LEFT_3_PENALTY=0.188254
PRIMER_RIGHT_3_PENALTY=0.095445
PRIMER_LEFT_3_SEQUENCE=CCAGTCTCCCACCTGCTACTAC
PRIMER_RIGHT_3_SEQUENCE=GATAGTTGATGGCTTTTCGGAG
PRIMER_LEFT_3=602,22
PRIMER_RIGHT_3=847,22
PRIMER_LEFT_3_TM=60.188
PRIMER_RIGHT_3_TM=60.095
PRIMER_LEFT_3_GC_PERCENT=59.091
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.1000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=0.2995
PRIMER_LEFT_4_PENALTY=0.232060
PRIMER_RIGHT_4_PENALTY=0.067430
PRIMER_LEFT_4_SEQUENCE=GCAAAGTTGATTTCGACCTTCT
PRIMER_RIGHT_4_SEQUENCE=AATATCAGCAGCAGGCACAGTA
PRIMER_LEFT_4=515,22
PRIMER_RIGHT_4=786,22
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=59.933
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_00546 Ps_contig_mira-and-tgicl-ass_franssen_5278
SEQUENCE=GATAATGGTGATCTGAAAATCTTTTATTTGAGAGATATCCAAAGCTAATCTCGTTGCGAAGGTGGGCGTGGTCATCAAAGCTAATTTGGTTATGGTCATCATGTATCCGTTATTGCAGCATCATGTTATGTTTAGGAATACTTGTTGCATGAAGCAAAATCAACAGCGGCAGTATGCATCGTTGAGAAGCAATGAAAAGCAGCAGCAGCGGCAGTATGCATCGGCGGCGGCGAGAGACAGAGTAATAGATTTCGGTAAATATAAGGGGAAGATGCTGGGAAGCCTTCCATCAAGCTATCTGAAATGGGTTTCAAAAAACTTACGTGCACGGGATTTTGAGAAATGGGCAATTCTTGCCGATCAAGTCCTTGTGGACCCAATCTACAAAGACCGCATCGAGTGGGAGTTTGCTCTCAATGTGTTAGAAAGAAACAGATCCGGTGAGCCCGCAGTTTCTCAACTACAGGACATAAGCGACAGGTTTGGTTGGGATTATCTTGACAAACTTGGTTGGAGCAAAGTTGATTTCGACCTTCTCGGCACTTCTAATGGAGGGAGAATTCCGAGACTAGACAATCCAAGGGACGCCGACGCCAGTCAACCCAGTCTCCCACCTGCTACTACTACTAGTAACAATAGACCCAATCTCCGACCTGCTACTACTAGTAGGAGGATGGAGAGAAGAGAGAAACTGAAAATGAGATCGAGGACGACACATGATGAAGAAGAAGAAGAAGCGCAGCAGCAGCACAAGGACAAGGACGATACTGTGCCTGCTGCTGATATTAAACAAATTGCCAACCCATTCCCTGGACGTCAGGCTCTCCTCCGAAAAGCCATCAACTATCGCGCACAATCCTAAACAACTTTCTATCATCCATCTACTCTAATTAACTAATTTCCTTTTTTACTTTTTTCTTTTAATTAAGATTCTTGTATGTTATTACCCG
TARGET=739,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2142
PRIMER_LEFT_PENALTY=0.118777
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=GAGGACGACACATGATGAAGAA
PRIMER_RIGHT_SEQUENCE=GATAGTTGATGGCTTTTCGGAG
PRIMER_LEFT=705,22
PRIMER_RIGHT=847,22
PRIMER_LEFT_TM=60.119
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=143
PRIMER_PAIR_PENALTY_1=0.2142
PRIMER_LEFT_1_PENALTY=0.118777
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=GGACGACACATGATGAAGAAGA
PRIMER_RIGHT_1_SEQUENCE=GATAGTTGATGGCTTTTCGGAG
PRIMER_LEFT_1=707,22
PRIMER_RIGHT_1=847,22
PRIMER_LEFT_1_TM=60.119
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.2185
PRIMER_LEFT_2_PENALTY=0.151106
PRIMER_RIGHT_2_PENALTY=0.067430
PRIMER_LEFT_2_SEQUENCE=CCAATCTCCGACCTGCTACTAC
PRIMER_RIGHT_2_SEQUENCE=AATATCAGCAGCAGGCACAGTA
PRIMER_LEFT_2=641,22
PRIMER_RIGHT_2=786,22
PRIMER_LEFT_2_TM=60.151
PRIMER_RIGHT_2_TM=59.933
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.1000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.2185
PRIMER_LEFT_3_PENALTY=0.151106
PRIMER_RIGHT_3_PENALTY=0.067430
PRIMER_LEFT_3_SEQUENCE=CCAATCTCCGACCTGCTACTAC
PRIMER_RIGHT_3_SEQUENCE=TAATATCAGCAGCAGGCACAGT
PRIMER_LEFT_3=641,22
PRIMER_RIGHT_3=787,22
PRIMER_LEFT_3_TM=60.151
PRIMER_RIGHT_3_TM=59.933
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.1000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=0.2557
PRIMER_LEFT_4_PENALTY=0.188254
PRIMER_RIGHT_4_PENALTY=0.067430
PRIMER_LEFT_4_SEQUENCE=CCAGTCTCCCACCTGCTACTAC
PRIMER_RIGHT_4_SEQUENCE=AATATCAGCAGCAGGCACAGTA
PRIMER_LEFT_4=602,22
PRIMER_RIGHT_4=786,22
PRIMER_LEFT_4_TM=60.188
PRIMER_RIGHT_4_TM=59.933
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_00547 Ps_contig_mira-and-tgicl-ass_franssen_5282
SEQUENCE=AGAAGCACCCTATCATTTKGTATCGTTATCTTCTCAAGTATTGATATAACCCTAGCTCCGAACACCGCTTTCTTCTTTTTCTAATCATCTCATTTTCTTCTCTCTCCAAACCCTAATACCAATTTTCCATTTTCACTCCAACTTCGTTGTATCAATTTCTCTCCATTCATTTCCTTCTAGATCTCAAACTCTTCCTTTTCTTTTTCTCGATTAAATCAAATAATACAAAAGCTTCGTTCTTTCTATAACCGCTTCACCTTTTTGAATTTCAGCGATATATAAAACCCGATTCAGTCCCAATTTTGATTTCAACCGACAACAACACAAGCGGCGAGGTTTGGAATTTCCGAGAGTAGTGAAAATCGCTGTTGACAATGGTTGGCGGTGGAAGCAGAAGAGATGATGGGTCGTTGGTAATTAACAACACCAACGTGTTTGCGGCGCTTGATACTTTGAAGAAGAAAAAGAAATCGGATAAGGAGAGAAAGAATAAGGGTTCCAAATCCACCAAATTGGAATCTGAATCGGATTCAAAGGTGTTTTGGGCTCCGGCCCCATTGAATGCATCTTCATGGGCTGATGTGGATGATGATGATGATTATTATGCCACCACTGCACCGCCTCAATCTGTTTGGTCTGTTTCCGACCCCCAAAACAGTGAGGACAAGCCAGAGATTTTTGAGGATACTGAGAGTGAGGAAGATATTTTAGATGATGGGGATGAGGAGGAGGAAGAGCAAGATCATGAACCAGAACCAGAGGACGCTGTGAAACCTGAACCTGAGCTCAAGAAGCATGCTGAAGTTCCTGTGGCACCAAAGGAAGCTGAACGGCAGCTCTCCAAAAAGGAAAGGAAGAAAAAGGAACTTGAAGAGCTTGAGGCTCTTTTAGCTGATTTTGGAGTCGCACAGAAAGAAAGTAACGATGGTCAAGGTCAAGATGAGTCGCAAGGCGCACCTGAGGATAAGAAAGATGTTGAAGGGGATGTAAATGGAGAAAAGAAAGAAATAGCTGTGGAGTCAAAGAATGCCAAGAAGAAGAAAAAGAAGGAAAAAGCTTCCAAGGAGGTGAAAGAATCCAACGGTCAGCACAGCAATTCAGAAACAAACGTTGTGTCTGATATGGCCGAAAATGCGGAGGAGGATCCATCTGTTGTTGATGTGAAAGAGCGTCTTAAGAAACTTACTTCTATCAAGAAGAAGAAATCCAATAAAGAAATGGATGGTGCTGCTAGAGCTGCTGCTCAAGAAGCTGCTGCAAGGAATGCAAGGCTTGCTGCAGCAAAGAAAAAAGAGAAGAACCATTATAACCAACAACCTGTGCGGTAAAAGAATCTTGCTCCTAAACATCTATCTATACAGGAATTTGTATTTCTTGTCTATATGATGATGATGATGATGATGATGCCGAATAAATAGTCGGGCTTTAGGCTCTGGTTACCCTAGCCGTACTCCAGTAGACTTGATATACTGTCTTATGCTTTTTTGGGCTAGTGTTGTTTAATTGTTATAGACCATTATATTTTGAGCACGGGATTTTATCTCTAAAAATTACAAGAACTATGTTTTGAAAATCCCATGGGACTAAGTTGTTGTGCATTTGTTCTTATGTCGTGGCTACTGCTGGTGCGAGTAGTTGAAGTTTAGTTTTGAAAGTGTTGTGTTGTGTACTACAGACAGATTAAAAAATATTCTTCAAGAAAA
TARGET=585,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00548 Ps_contig_mira-and-tgicl-ass_franssen_5282
SEQUENCE=AGAAGCACCCTATCATTTKGTATCGTTATCTTCTCAAGTATTGATATAACCCTAGCTCCGAACACCGCTTTCTTCTTTTTCTAATCATCTCATTTTCTTCTCTCTCCAAACCCTAATACCAATTTTCCATTTTCACTCCAACTTCGTTGTATCAATTTCTCTCCATTCATTTCCTTCTAGATCTCAAACTCTTCCTTTTCTTTTTCTCGATTAAATCAAATAATACAAAAGCTTCGTTCTTTCTATAACCGCTTCACCTTTTTGAATTTCAGCGATATATAAAACCCGATTCAGTCCCAATTTTGATTTCAACCGACAACAACACAAGCGGCGAGGTTTGGAATTTCCGAGAGTAGTGAAAATCGCTGTTGACAATGGTTGGCGGTGGAAGCAGAAGAGATGATGGGTCGTTGGTAATTAACAACACCAACGTGTTTGCGGCGCTTGATACTTTGAAGAAGAAAAAGAAATCGGATAAGGAGAGAAAGAATAAGGGTTCCAAATCCACCAAATTGGAATCTGAATCGGATTCAAAGGTGTTTTGGGCTCCGGCCCCATTGAATGCATCTTCATGGGCTGATGTGGATGATGATGATGATTATTATGCCACCACTGCACCGCCTCAATCTGTTTGGTCTGTTTCCGACCCCCAAAACAGTGAGGACAAGCCAGAGATTTTTGAGGATACTGAGAGTGAGGAAGATATTTTAGATGATGGGGATGAGGAGGAGGAAGAGCAAGATCATGAACCAGAACCAGAGGACGCTGTGAAACCTGAACCTGAGCTCAAGAAGCATGCTGAAGTTCCTGTGGCACCAAAGGAAGCTGAACGGCAGCTCTCCAAAAAGGAAAGGAAGAAAAAGGAACTTGAAGAGCTTGAGGCTCTTTTAGCTGATTTTGGAGTCGCACAGAAAGAAAGTAACGATGGTCAAGGTCAAGATGAGTCGCAAGGCGCACCTGAGGATAAGAAAGATGTTGAAGGGGATGTAAATGGAGAAAAGAAAGAAATAGCTGTGGAGTCAAAGAATGCCAAGAAGAAGAAAAAGAAGGAAAAAGCTTCCAAGGAGGTGAAAGAATCCAACGGTCAGCACAGCAATTCAGAAACAAACGTTGTGTCTGATATGGCCGAAAATGCGGAGGAGGATCCATCTGTTGTTGATGTGAAAGAGCGTCTTAAGAAACTTACTTCTATCAAGAAGAAGAAATCCAATAAAGAAATGGATGGTGCTGCTAGAGCTGCTGCTCAAGAAGCTGCTGCAAGGAATGCAAGGCTTGCTGCAGCAAAGAAAAAAGAGAAGAACCATTATAACCAACAACCTGTGCGGTAAAAGAATCTTGCTCCTAAACATCTATCTATACAGGAATTTGTATTTCTTGTCTATATGATGATGATGATGATGATGATGCCGAATAAATAGTCGGGCTTTAGGCTCTGGTTACCCTAGCCGTACTCCAGTAGACTTGATATACTGTCTTATGCTTTTTTGGGCTAGTGTTGTTTAATTGTTATAGACCATTATATTTTGAGCACGGGATTTTATCTCTAAAAATTACAAGAACTATGTTTTGAAAATCCCATGGGACTAAGTTGTTGTGCATTTGTTCTTATGTCGTGGCTACTGCTGGTGCGAGTAGTTGAAGTTTAGTTTTGAAAGTGTTGTGTTGTGTACTACAGACAGATTAAAAAATATTCTTCAAGAAAA
TARGET=1383,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00549 Ps_contig_mira-and-tgicl-ass_franssen_5300
SEQUENCE=TCAACGGTTATTCCCTCTCCTCCATTTTCAGCCATGTCGACCGTCGCTCAAACCAAGCCCAAGAAAACCGCTGCAGCAAAGAAGCCACTTTCTCATCCCACTTTCGCTGAGGTAGAAATCAAAATCCCGTTCAATTTTCAGTATTTTTGAATATTTTTTTTGGGTGTGTATATTGATTTTGTTTTTTCTTATTTCCTTTTGAAGATGATAACTGAAGCAATCACAAGCCTGAAAGAAAGAACCGGTTCAAGCCAATATGCTATAACAAAATTCATCGAAGAGAAATACAAGGACTTACCTCCAACTTACCGTAAGCTTGTTCTCCTCCATCTCAAGAAATCCGTCGCTTCAGGAAAGCTTGTCAAGGTGAAAAGCTCCTTCAAACTCGCTCCGGCAGCCGCAAAAACTGCTCCGGCCAAGACATCGGCTGCTACCAAAGCTCCTAAAGCTGTTACCAAGCCAAGCGCTAAAGCTGTCACAAAGCCCAAGGCTAAAGCTGCTGGGAAGCCGAAAGCTGTTGCGAAGCCAAAGGCTAAGAGCGTGAAGGCTACTCCGGTGAAGAAGGCTGTTGCTAAGAAGGTTGTGAAGAAGGCGAAGAGCGTTAAAAGTCCGGCGAAGAAGGTGAAGAGCGTTAAAACTCCGGTGAAGAAGGCTAAGAAGTGAGGGATTAGGGTTTGATTTGATTTGATTGGGTTTTTTAGTTATGTGTGTAATGTTATGGTTTATTATTATTATTGG
TARGET=674,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00550 Ps_contig_mira-and-tgicl-ass_franssen_5300
SEQUENCE=TCAACGGTTATTCCCTCTCCTCCATTTTCAGCCATGTCGACCGTCGCTCAAACCAAGCCCAAGAAAACCGCTGCAGCAAAGAAGCCACTTTCTCATCCCACTTTCGCTGAGGTAGAAATCAAAATCCCGTTCAATTTTCAGTATTTTTGAATATTTTTTTTGGGTGTGTATATTGATTTTGTTTTTTCTTATTTCCTTTTGAAGATGATAACTGAAGCAATCACAAGCCTGAAAGAAAGAACCGGTTCAAGCCAATATGCTATAACAAAATTCATCGAAGAGAAATACAAGGACTTACCTCCAACTTACCGTAAGCTTGTTCTCCTCCATCTCAAGAAATCCGTCGCTTCAGGAAAGCTTGTCAAGGTGAAAAGCTCCTTCAAACTCGCTCCGGCAGCCGCAAAAACTGCTCCGGCCAAGACATCGGCTGCTACCAAAGCTCCTAAAGCTGTTACCAAGCCAAGCGCTAAAGCTGTCACAAAGCCCAAGGCTAAAGCTGCTGGGAAGCCGAAAGCTGTTGCGAAGCCAAAGGCTAAGAGCGTGAAGGCTACTCCGGTGAAGAAGGCTGTTGCTAAGAAGGTTGTGAAGAAGGCGAAGAGCGTTAAAAGTCCGGCGAAGAAGGTGAAGAGCGTTAAAACTCCGGTGAAGAAGGCTAAGAAGTGAGGGATTAGGGTTTGATTTGATTTGATTGGGTTTTTTAGTTATGTGTGTAATGTTATGGTTTATTATTATTATTGG
TARGET=723,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00551 Ps_contig_mira-and-tgicl-ass_franssen_5301
SEQUENCE=GTTCGGGGACATCATCACATTCTCTTCAATCTTCTCAATATCTCTCATTCTCTCTCATTCTCTCTCATCAACGGTTATTCCCTCTCCTCCATTTTCAGCCATGTCGACCGTCGCTCAAACCAAGCCCAAGAAAACTGCTGCGGCAAAGAAGCCACYTTCTCATCCCACTTTCGCTGAGATGATAACTGAAGCAATCACAAGCCTAGAAAGAAAGAACCGGTTCAAGCCAATATGCTATAACAAAGTTCATCGAAGAGAAACACAAGGACTTACCTCCAACTTACCGTAAGCTTGTTCTCCTCCATCTCAAGAAATCCGTCGCTTCAGGAAAGCTTGTCAGGGTGAAAAGCTCCTTCAAACTCGCTCCGGCGGCCGCAAAAACTGCTCCGGCCAAGACATCGGCTGCTACCAAAGCTCCTAAAGCTGTTACCAAGCCAAGCGCTAAAACTGTCACAAAGCCCAAGGCTAAAGCTGCKGGAAGCCGAAAGCCAAAGCTGCGGCAAAGCCGAAAGCTGTTGCGAAGCCAAAGGCTAAGAGCGTGAAGGCTACTCCGGTGAAGAAGGCTGTTGCTAAGAAGGTTGTGAAGAAGGCGAAGAGCGTTAAAAGTCCGGCGAAGAAGGTGAAGAGCGTTAAAACTCCGGTGAAGAAGGCTAAGAAGTGAGGGATTAGGGTTTGATTTGATTTGATTGGGTTTTTTAGTTATGTGTGTAATGTTATGGTTTTATTATTATTATGGTTTGGTTTTAGAAAGATTAGAGATGAAAATTAGTGTTAGGAGGTTGTTGCTGTAAATTGTTTAGGAGAAACACATCTTGTGTTCTTTGTACTCGACACCGTCTTAATGAAATGGGTTTTCTGTTTTCCYSA
TARGET=205,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00552 Ps_contig_mira-and-tgicl-ass_franssen_5301
SEQUENCE=GTTCGGGGACATCATCACATTCTCTTCAATCTTCTCAATATCTCTCATTCTCTCTCATTCTCTCTCATCAACGGTTATTCCCTCTCCTCCATTTTCAGCCATGTCGACCGTCGCTCAAACCAAGCCCAAGAAAACTGCTGCGGCAAAGAAGCCACYTTCTCATCCCACTTTCGCTGAGATGATAACTGAAGCAATCACAAGCCTAGAAAGAAAGAACCGGTTCAAGCCAATATGCTATAACAAAGTTCATCGAAGAGAAACACAAGGACTTACCTCCAACTTACCGTAAGCTTGTTCTCCTCCATCTCAAGAAATCCGTCGCTTCAGGAAAGCTTGTCAGGGTGAAAAGCTCCTTCAAACTCGCTCCGGCGGCCGCAAAAACTGCTCCGGCCAAGACATCGGCTGCTACCAAAGCTCCTAAAGCTGTTACCAAGCCAAGCGCTAAAACTGTCACAAAGCCCAAGGCTAAAGCTGCKGGAAGCCGAAAGCCAAAGCTGCGGCAAAGCCGAAAGCTGTTGCGAAGCCAAAGGCTAAGAGCGTGAAGGCTACTCCGGTGAAGAAGGCTGTTGCTAAGAAGGTTGTGAAGAAGGCGAAGAGCGTTAAAAGTCCGGCGAAGAAGGTGAAGAGCGTTAAAACTCCGGTGAAGAAGGCTAAGAAGTGAGGGATTAGGGTTTGATTTGATTTGATTGGGTTTTTTAGTTATGTGTGTAATGTTATGGTTTTATTATTATTATGGTTTGGTTTTAGAAAGATTAGAGATGAAAATTAGTGTTAGGAGGTTGTTGCTGTAAATTGTTTAGGAGAAACACATCTTGTGTTCTTTGTACTCGACACCGTCTTAATGAAATGGGTTTTCTGTTTTCCYSA
TARGET=672,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00553 Ps_contig_mira-and-tgicl-ass_franssen_5301
SEQUENCE=GTTCGGGGACATCATCACATTCTCTTCAATCTTCTCAATATCTCTCATTCTCTCTCATTCTCTCTCATCAACGGTTATTCCCTCTCCTCCATTTTCAGCCATGTCGACCGTCGCTCAAACCAAGCCCAAGAAAACTGCTGCGGCAAAGAAGCCACYTTCTCATCCCACTTTCGCTGAGATGATAACTGAAGCAATCACAAGCCTAGAAAGAAAGAACCGGTTCAAGCCAATATGCTATAACAAAGTTCATCGAAGAGAAACACAAGGACTTACCTCCAACTTACCGTAAGCTTGTTCTCCTCCATCTCAAGAAATCCGTCGCTTCAGGAAAGCTTGTCAGGGTGAAAAGCTCCTTCAAACTCGCTCCGGCGGCCGCAAAAACTGCTCCGGCCAAGACATCGGCTGCTACCAAAGCTCCTAAAGCTGTTACCAAGCCAAGCGCTAAAACTGTCACAAAGCCCAAGGCTAAAGCTGCKGGAAGCCGAAAGCCAAAGCTGCGGCAAAGCCGAAAGCTGTTGCGAAGCCAAAGGCTAAGAGCGTGAAGGCTACTCCGGTGAAGAAGGCTGTTGCTAAGAAGGTTGTGAAGAAGGCGAAGAGCGTTAAAAGTCCGGCGAAGAAGGTGAAGAGCGTTAAAACTCCGGTGAAGAAGGCTAAGAAGTGAGGGATTAGGGTTTGATTTGATTTGATTGGGTTTTTTAGTTATGTGTGTAATGTTATGGTTTTATTATTATTATGGTTTGGTTTTAGAAAGATTAGAGATGAAAATTAGTGTTAGGAGGTTGTTGCTGTAAATTGTTTAGGAGAAACACATCTTGTGTTCTTTGTACTCGACACCGTCTTAATGAAATGGGTTTTCTGTTTTCCYSA
TARGET=722,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00554 Ps_contig_mira-and-tgicl-ass_franssen_5305
SEQUENCE=CCGGGTAGGACGTCTAGGCGTTTCCAAAGTTAGGCGGCCGAGAAGGAAAGGGTTAGGTTTGGTCAGAAGACCACTGGTTCTTTGTACACCACTTCTGTCTTCTTCTTCTCCTCGTTTAAAGTTMYTCCATCTTTCCGTTAGACCTAAGGATGGACTTGGTAGTTGGTCAACACTTTTCCAGAGGCACCGCTACCTCTTATCTGCTCTCGTGCTCCTTACTATTCTATGTACTGTTTATCTTTACTTCGCCATCACTATRGGAAGTGGCTCCTGTTCTGGGTTGAGTGGAGCACAGAAGCTTCATGCCATATGGAGCTTTGGTACCAAGGTNTTTCTTACTTTTACCGAAGTTCGGG
TARGET=98,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00555 Ps_contig_mira-and-tgicl-ass_franssen_5318
SEQUENCE=CGAACAATAGAACTGCAACAGATATTAACACCTTCCAATTCTTCATCTTGGAAGGTGGCCAATTCCCCTTCTTCCTCAACCTCCTCTTTTGAACAAGCCTCTTTCTCTTCAACCTTATCTTCTTGGCAAGCTTCATCTTCAACATCCGCTCTTCCCTCTCCTTCAATTTTGATTCTAATGGAAGCTTATCTACAGAAACAACATCACTCTCTTTCTTGCTCTCACTTTCTTCTACTCCATCTGCAACATTAGAATCAGCAGCAGCAGCAGCTGATGCAATAAAAATTGCATTTTTTCTCGGTTTCACAACAATGGGTGTCAAGATTCGAAATCCCTTCAAACAGTTGGTGGGCAGACCGATTCGTACTCTAGAAATAGGGTTTGCTGTGGGATGAAGGAAAAGGGAGGAAGAAGAGGTAACAGGGGTGGTTGTGAAGGAATTGAAGCAGAGAAGTGCCATAGTGTTTGGTTTTTTATTTTTCTTTTTCAAATGAAAAATTGGTTTATATGATGTTATGTGGTATGTTATGTTAAGGATAAGAAAGGCACATCAGACGTGTCTTCTTCCATGCTCACCACTCACCCCGA
TARGET=256,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3365
PRIMER_LEFT_PENALTY=0.142170
PRIMER_RIGHT_PENALTY=0.194358
PRIMER_LEFT_SEQUENCE=TCTTCCTCAACCTCCTCTTTTG
PRIMER_RIGHT_SEQUENCE=GATTTCGAATCTTGACACCCAT
PRIMER_LEFT=69,22
PRIMER_RIGHT=332,22
PRIMER_LEFT_TM=59.858
PRIMER_RIGHT_TM=60.194
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.3365
PRIMER_LEFT_1_PENALTY=0.142170
PRIMER_RIGHT_1_PENALTY=0.194358
PRIMER_LEFT_1_SEQUENCE=CTTCCTCAACCTCCTCTTTTGA
PRIMER_RIGHT_1_SEQUENCE=GATTTCGAATCTTGACACCCAT
PRIMER_LEFT_1=70,22
PRIMER_RIGHT_1=332,22
PRIMER_LEFT_1_TM=59.858
PRIMER_RIGHT_1_TM=60.194
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.3782
PRIMER_LEFT_2_PENALTY=0.202525
PRIMER_RIGHT_2_PENALTY=0.175636
PRIMER_LEFT_2_SEQUENCE=GCTCTTCCCTCTCCTTCAATTT
PRIMER_RIGHT_2_SEQUENCE=TCTTCTTCCTCCCTTTTCCTTC
PRIMER_LEFT_2=147,22
PRIMER_RIGHT_2=414,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=60.176
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.3966
PRIMER_LEFT_3_PENALTY=0.142170
PRIMER_RIGHT_3_PENALTY=0.254467
PRIMER_LEFT_3_SEQUENCE=TCTTCCTCAACCTCCTCTTTTG
PRIMER_RIGHT_3_SEQUENCE=CACCAACTGTTTGAAGGGATTT
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=349,22
PRIMER_LEFT_3_TM=59.858
PRIMER_RIGHT_3_TM=60.254
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.3966
PRIMER_LEFT_4_PENALTY=0.142170
PRIMER_RIGHT_4_PENALTY=0.254467
PRIMER_LEFT_4_SEQUENCE=CTTCCTCAACCTCCTCTTTTGA
PRIMER_RIGHT_4_SEQUENCE=CACCAACTGTTTGAAGGGATTT
PRIMER_LEFT_4=70,22
PRIMER_RIGHT_4=349,22
PRIMER_LEFT_4_TM=59.858
PRIMER_RIGHT_4_TM=60.254
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_00556 Ps_contig_mira-and-tgicl-ass_franssen_5335
SEQUENCE=CGTTTATTTCATCTTAATATATCATTGAGTATCAATAAGCTTAGATCATCCATCATATACAATTACATAATACGCACTAATATAGAAAGTACAATGGAAGGATAAATTGCTGAAAATGTGAGATTTGAGAGAATCCCAATTGAAATCTTACCATCCCCAGAAGGACCAATCAGGAAAAGGTATATCAAAATGTTCCTTTGTGGTACAATATCGAAAGAGTGGCAAAGGCTTTGAATTATGTTCAGTTTTGTTAATACTAGGCTTATCCATGCAATCAAACATCAAATCCACATCAGGAACCAACCCAGGGTACTTCCTCAAAAGCTGCAACAAACCCCAAACAGTAAACATAGCCCTACTCTGAACACAAGCATAATACCAATCAACAAACATCTTCCCTCCAACAATCACCACTCTAAAAGCCGCATACTTTTGCGCTTCCGCCAAATGCCCCTCCGAAATTCTGGTTTTCATCCACGGCTCCAGATCTCTCCGGATCGCCTTGAAAAAATCAGGACAAGTCGGCGCTTTCGCTTTCGCATCTCCGGCAGCGTAACCGAGTCGTCCGCTGCCACCGGAGACCGCGTTACGGCATGTGAGATATGAACACTGTATGATTTTGTAGGCTCTGCTCTGACGAGTCTCTTCGTCGAATGGTTTCGTAGGGAAAACGTGCCACGGCGTTGGTTCTAGGTTGTGACCTACCACGGTTCCGGTTCGGCTGACAACGTCGTCCACCTTGTAGAGAAGAAGGCCAGTAAGAGAGAAGAAAGAGAGAGCGATGACACATGGAAAGAGATAAGTTGGGGTACGAGGAGAGTGTTTGGGAGAGAGACCCATGATAGAAGAAGAAGAAAAAGTGAAGAAGAAGAAAGAATTGTTGTGAGTGTTTTGAGACATTGTGCTGTGAGTGTKWGWAAATTTGAGGTGACTTGGAAAATATGGACACTCAATTCACGTGACGGTGGAGACCACGTGTGTGAAAAAAGTC
TARGET=844,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00557 Ps_contig_mira-and-tgicl-ass_franssen_5335
SEQUENCE=CGTTTATTTCATCTTAATATATCATTGAGTATCAATAAGCTTAGATCATCCATCATATACAATTACATAATACGCACTAATATAGAAAGTACAATGGAAGGATAAATTGCTGAAAATGTGAGATTTGAGAGAATCCCAATTGAAATCTTACCATCCCCAGAAGGACCAATCAGGAAAAGGTATATCAAAATGTTCCTTTGTGGTACAATATCGAAAGAGTGGCAAAGGCTTTGAATTATGTTCAGTTTTGTTAATACTAGGCTTATCCATGCAATCAAACATCAAATCCACATCAGGAACCAACCCAGGGTACTTCCTCAAAAGCTGCAACAAACCCCAAACAGTAAACATAGCCCTACTCTGAACACAAGCATAATACCAATCAACAAACATCTTCCCTCCAACAATCACCACTCTAAAAGCCGCATACTTTTGCGCTTCCGCCAAATGCCCCTCCGAAATTCTGGTTTTCATCCACGGCTCCAGATCTCTCCGGATCGCCTTGAAAAAATCAGGACAAGTCGGCGCTTTCGCTTTCGCATCTCCGGCAGCGTAACCGAGTCGTCCGCTGCCACCGGAGACCGCGTTACGGCATGTGAGATATGAACACTGTATGATTTTGTAGGCTCTGCTCTGACGAGTCTCTTCGTCGAATGGTTTCGTAGGGAAAACGTGCCACGGCGTTGGTTCTAGGTTGTGACCTACCACGGTTCCGGTTCGGCTGACAACGTCGTCCACCTTGTAGAGAAGAAGGCCAGTAAGAGAGAAGAAAGAGAGAGCGATGACACATGGAAAGAGATAAGTTGGGGTACGAGGAGAGTGTTTGGGAGAGAGACCCATGATAGAAGAAGAAGAAAAAGTGAAGAAGAAGAAAGAATTGTTGTGAGTGTTTTGAGACATTGTGCTGTGAGTGTKWGWAAATTTGAGGTGACTTGGAAAATATGGACACTCAATTCACGTGACGGTGGAGACCACGTGTGTGAAAAAAGTC
TARGET=862,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00558 Ps_contig_mira-and-tgicl-ass_franssen_5352
SEQUENCE=ATCTCCGACAAAGTTACTAATACTTTGTAGGTAAAGGTAACGGAAGTTACTTCTTCTTTCTAATTGGGAACGTTAGGTTCGGTATGTCGTTTGGTACGGCCGGAACGGAAAGGAAAGGAAAGGAAAATAAGGTTCGAATAGGTGGTTTGGTACGGAAGGAAAGTACTTTTCTATTTTCTTTTGGGTCGTGTTTTCTTTCTTCCAATCGGTGACCCAATTGAAGGAATGAACCACCGTCTTCTTCATCTCGATGATGGGTTCAGTGAGGAACAGGTTAAGGTTAACCATGATATTGATGATGATGATGATGAAGAAGAAGACGACGATGATGATGATGAAGTATTTATTTGGATGATGAGTCTTTGTTCCAGTGGGATAGTARGGAAACTAAAGTTGGGAAANACAGGAAGAATAGGAAGGTAAAAGGTTTAGGGGGTAAAACGGTTTCAATACAAAAGATAGAAACTAAGTTACGG
TARGET=107,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00559 Ps_contig_mira-and-tgicl-ass_franssen_5352
SEQUENCE=ATCTCCGACAAAGTTACTAATACTTTGTAGGTAAAGGTAACGGAAGTTACTTCTTCTTTCTAATTGGGAACGTTAGGTTCGGTATGTCGTTTGGTACGGCCGGAACGGAAAGGAAAGGAAAGGAAAATAAGGTTCGAATAGGTGGTTTGGTACGGAAGGAAAGTACTTTTCTATTTTCTTTTGGGTCGTGTTTTCTTTCTTCCAATCGGTGACCCAATTGAAGGAATGAACCACCGTCTTCTTCATCTCGATGATGGGTTCAGTGAGGAACAGGTTAAGGTTAACCATGATATTGATGATGATGATGATGAAGAAGAAGACGACGATGATGATGATGAAGTATTTATTTGGATGATGAGTCTTTGTTCCAGTGGGATAGTARGGAAACTAAAGTTGGGAAANACAGGAAGAATAGGAAGGTAAAAGGTTTAGGGGGTAAAACGGTTTCAATACAAAAGATAGAAACTAAGTTACGG
TARGET=294,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00560 Ps_contig_mira-and-tgicl-ass_franssen_5352
SEQUENCE=ATCTCCGACAAAGTTACTAATACTTTGTAGGTAAAGGTAACGGAAGTTACTTCTTCTTTCTAATTGGGAACGTTAGGTTCGGTATGTCGTTTGGTACGGCCGGAACGGAAAGGAAAGGAAAGGAAAATAAGGTTCGAATAGGTGGTTTGGTACGGAAGGAAAGTACTTTTCTATTTTCTTTTGGGTCGTGTTTTCTTTCTTCCAATCGGTGACCCAATTGAAGGAATGAACCACCGTCTTCTTCATCTCGATGATGGGTTCAGTGAGGAACAGGTTAAGGTTAACCATGATATTGATGATGATGATGATGAAGAAGAAGACGACGATGATGATGATGAAGTATTTATTTGGATGATGAGTCTTTGTTCCAGTGGGATAGTARGGAAACTAAAGTTGGGAAANACAGGAAGAATAGGAAGGTAAAAGGTTTAGGGGGTAAAACGGTTTCAATACAAAAGATAGAAACTAAGTTACGG
TARGET=325,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00561 Ps_contig_mira-and-tgicl-ass_franssen_5382
SEQUENCE=AGCAGTGGGATCAACGCAGAGTTCGGCTAGGGTTCTAGAGCAGGAAACTCATAATAAGAAGTTGAGACAAAAAAATTATGAGATCTGCTGAATAACTATCTTCAATGGTGGAAATTACTTCTGTTCTTTCAGATATGATATGGCAATGGACAATGTTCAGAGATATGCACCTGACTTGGATCATCAAGTTTCTTTTGAAATCACATGAAACTTGAGTTTTCCTTTTTGAACTTTTGGGTGAACGTGTAGTATATGTGGCGCAGATGTTATTGTATTTTACTTTTTGGCTACTCTTCAAGGTGAAGAAGGTATTGATTGTGCAACTTTGTTGAATGATTTGTGCCTTCATAATATTCGAAAGGTCCTGATGTTCTTGTCTCCCTGCACCACCATTTACTTCATGTAATGATATGCCAACTGTCTTCTATATTGTGTAACTCTTTCTTTCTTTTAGCTCTTCA
TARGET=439,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00562 Ps_contig_mira-and-tgicl-ass_franssen_5388
SEQUENCE=AGATTTGTAGAGAGGAAGGTGTGGAACAGGTTTTTGTTGATGCTGCTCATTCTATTGGGTGTACTGATGTTGACATGCAGGAGATTGGTGCTGATTATTACACTAGTAATTTGCACAAGTTGGTTTTTTTGTCCGCCTTCGATTGTCGTTTTTGTACTCGAAGAAAAAACACTAAACTACTGGTGGTGGTGGTTTGCATCATCCTGTGGTGTCTCATAGAGTATGGAAATGGGTTGGCTGTAGGAAAGTGCTTGGAGTTAGGG
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1368
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.050443
PRIMER_LEFT_SEQUENCE=GTGTGGAACAGGTTTTTGTTGA
PRIMER_RIGHT_SEQUENCE=ACTCCAAGCACTTTCCTACAGC
PRIMER_LEFT=18,22
PRIMER_RIGHT=257,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=59.950
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.1920
PRIMER_LEFT_1_PENALTY=0.141511
PRIMER_RIGHT_1_PENALTY=0.050443
PRIMER_LEFT_1_SEQUENCE=CTGATGTTGACATGCAGGAGAT
PRIMER_RIGHT_1_SEQUENCE=ACTCCAAGCACTTTCCTACAGC
PRIMER_LEFT_1=63,22
PRIMER_RIGHT_1=257,22
PRIMER_LEFT_1_TM=60.142
PRIMER_RIGHT_1_TM=59.950
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=0.2160
PRIMER_LEFT_2_PENALTY=0.165541
PRIMER_RIGHT_2_PENALTY=0.050443
PRIMER_LEFT_2_SEQUENCE=TCGATTGTCGTTTTTGTACTCG
PRIMER_RIGHT_2_SEQUENCE=ACTCCAAGCACTTTCCTACAGC
PRIMER_LEFT_2=138,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=60.166
PRIMER_RIGHT_2_TM=59.950
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=0.2400
PRIMER_LEFT_3_PENALTY=0.189529
PRIMER_RIGHT_3_PENALTY=0.050443
PRIMER_LEFT_3_SEQUENCE=AGGTGTGGAACAGGTTTTTGTT
PRIMER_RIGHT_3_SEQUENCE=ACTCCAAGCACTTTCCTACAGC
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=59.810
PRIMER_RIGHT_3_TM=59.950
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.2808
PRIMER_LEFT_4_PENALTY=0.230329
PRIMER_RIGHT_4_PENALTY=0.050443
PRIMER_LEFT_4_SEQUENCE=TTGATGCTGCTCATTCTATTGG
PRIMER_RIGHT_4_SEQUENCE=ACTCCAAGCACTTTCCTACAGC
PRIMER_LEFT_4=36,22
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=60.230
PRIMER_RIGHT_4_TM=59.950
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_00563 Ps_contig_mira-and-tgicl-ass_franssen_5392
SEQUENCE=CTTAAACTTACATAATTATCTGCATGTTAAAGACAATATAAAAATAGAATAACCYWCACACTAAATCAAACTCCATTAAACTAAAATAAATCTAAGCAAACTAATATTATGACTATTTACAAATTATCTACAGAGTACCGGCTAAAACATGATAAAACAATCACAGTCCAATTTAATTGAAATTTATATTCCACAACTTTAACGCAATTGAACTTAAGATCGAAAATTTACCGACTCCAGATTTGAACTTGTTGACCTCAGACCGACTAATGCACGAACAAAAGTCAACGTAACGCGGAGGTTGTCGTGTGTATCTGTGAATGAATCTTTGTAAAGGGAGGTCGGAGAAATTGCAGATCGGAGGGTATATGGCGGTCCGACGATGAAACGGGGGCAAAGGAGCGATTTAAGGTGTTTACGGTGATGGTATTTTCGCCGGCTACAGTTAGGAGTAGATTTTGAGTTTTACAAGTTTGAGGATGTTGTTCCACGGCGAGTTGAAATCGGAGGCGGTTTTGAGTTATCGGTGAAACGCAACGGCAAAGGGAGCTTTATGGAATCCGCAGTGGATTTGACGGTGGCGGAAGATGAAGAGGTTCGTGGTGGTGGGGATGAGTGATACCGGCGCAATCGACGAGTCGGCGGCGACGGAGGTATGTGTGTCGTGGCGGCGGAGGATTTCACCGGCAATGGATGGAGTTTGGTCGGTGGTCGCCGGAACACGGAGAGGGTTTGAGCAAGCTTTTCGCCGGAATTGGTGGCGGATGGAGAGAGGATGGTGTTTGGTTTTTAAGGTCTGTTTTCTTTGTATTTTTGTTTGTGAGATTTTCAAAGAATAGGTAGCTTTTGGTTAAGGAGGAGAGATTGGGTGGGAAAGAAGAGAGGACGTGTGAATCTGTGTGTCSCCTTGTTCTTTTTGTTTTTCTTTTTAATGTACAGAGAGAGAGGGGTAACGTGGAAAGGTGAGGTAG
TARGET=938,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00564 Ps_contig_mira-and-tgicl-ass_franssen_5414
SEQUENCE=GGTTTTTAGTTTTCTTAGTACTTGAGGGTCTTGCGCGTAATTTTTGTAAAGCCTGTAATAAGGTGGTGGTGGTGGATATGTTGCCGTTGCCATAACAAAAAATCTTCCAATCAAAAACCGTTAAAGATCATACATGCATCTCTACTTCAAGATTTGACAGAGTTGTGTAGATGGAGGGCCGGCCGCCGGTGTGATGTCTGCGGCTGAGAAGCTGACTGGTGATGTGCTGGGCAGCTGTCGGCT
TARGET=62,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4316
PRIMER_LEFT_PENALTY=0.954958
PRIMER_RIGHT_PENALTY=0.476630
PRIMER_LEFT_SEQUENCE=CGCGTAATTTTTGTAAAGCCTG
PRIMER_RIGHT_SEQUENCE=ACATCACCAGTCAGCTTCTCAG
PRIMER_LEFT=33,22
PRIMER_RIGHT=224,22
PRIMER_LEFT_TM=60.955
PRIMER_RIGHT_TM=59.523
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=1.5216
PRIMER_LEFT_1_PENALTY=1.044969
PRIMER_RIGHT_1_PENALTY=0.476630
PRIMER_LEFT_1_SEQUENCE=TCTTAGTACTTGAGGGTCTTGCG
PRIMER_RIGHT_1_SEQUENCE=ACATCACCAGTCAGCTTCTCAG
PRIMER_LEFT_1=12,23
PRIMER_RIGHT_1=224,22
PRIMER_LEFT_1_TM=59.955
PRIMER_RIGHT_1_TM=59.523
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=1.7940
PRIMER_LEFT_2_PENALTY=1.317328
PRIMER_RIGHT_2_PENALTY=0.476630
PRIMER_LEFT_2_SEQUENCE=GGTCTTGCGCGTAATTTTTGTA
PRIMER_RIGHT_2_SEQUENCE=ACATCACCAGTCAGCTTCTCAG
PRIMER_LEFT_2=26,22
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=61.317
PRIMER_RIGHT_2_TM=59.523
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=2.0004
PRIMER_LEFT_3_PENALTY=0.954958
PRIMER_RIGHT_3_PENALTY=1.045432
PRIMER_LEFT_3_SEQUENCE=CGCGTAATTTTTGTAAAGCCTG
PRIMER_RIGHT_3_SEQUENCE=CCCTCCATCTACACAACTCTGTC
PRIMER_LEFT_3=33,22
PRIMER_RIGHT_3=177,23
PRIMER_LEFT_3_TM=60.955
PRIMER_RIGHT_3_TM=60.045
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=2.0210
PRIMER_LEFT_4_PENALTY=0.954958
PRIMER_RIGHT_4_PENALTY=1.066065
PRIMER_LEFT_4_SEQUENCE=CGCGTAATTTTTGTAAAGCCTG
PRIMER_RIGHT_4_SEQUENCE=GCCCTCCATCTACACAACTCTG
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=60.955
PRIMER_RIGHT_4_TM=61.066
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_00565 Ps_contig_mira-and-tgicl-ass_franssen_5415
SEQUENCE=GAAAGTTGGTTCGGAAAAACCCTAGACGGGGGAGTAGTGGAGTTTGAGTTGAGTTCAGGGTTTACTTTCAATTTCAATTCAGTACCAACTAAACCTATTGACTCTCTCTCTCTCTCTCTCTCTGCAATGGCAGTTTCAACGGAATTTCACCTTCCACGTCTCTCCGTCGTCGACTTTCCCTCTCTTCTCCAAGCTATGGCAGCCGACACTCTTCTTGCCGCTGCTCAATCTCTCGCTCTCATTGGATATCCGCTGGCTAATTTCACCAGTTTAGTGTCTCCTAAA
TARGET=102,22
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1693
PRIMER_LEFT_PENALTY=0.145769
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=GGAGTTTGAGTTGAGTTCAGGG
PRIMER_RIGHT_SEQUENCE=CATAGCTTGGAGAAGAGAGGGA
PRIMER_LEFT=38,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=60.146
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=0.3401
PRIMER_LEFT_1_PENALTY=0.145769
PRIMER_RIGHT_1_PENALTY=0.194358
PRIMER_LEFT_1_SEQUENCE=GGAGTTTGAGTTGAGTTCAGGG
PRIMER_RIGHT_1_SEQUENCE=GGATATCCAATGAGAGCGAGAG
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=250,22
PRIMER_LEFT_1_TM=60.146
PRIMER_RIGHT_1_TM=60.194
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.5146
PRIMER_LEFT_2_PENALTY=0.145769
PRIMER_RIGHT_2_PENALTY=0.368866
PRIMER_LEFT_2_SEQUENCE=GGAGTTTGAGTTGAGTTCAGGG
PRIMER_RIGHT_2_SEQUENCE=GCTTGGAGAAGAGAGGGAAAGT
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=193,22
PRIMER_LEFT_2_TM=60.146
PRIMER_RIGHT_2_TM=60.369
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.5360
PRIMER_LEFT_3_PENALTY=0.145769
PRIMER_RIGHT_3_PENALTY=0.390220
PRIMER_LEFT_3_SEQUENCE=GGAGTTTGAGTTGAGTTCAGGG
PRIMER_RIGHT_3_SEQUENCE=TAGCTTGGAGAAGAGAGGGAAA
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=60.146
PRIMER_RIGHT_3_TM=59.610
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=0.6347
PRIMER_LEFT_4_PENALTY=0.145769
PRIMER_RIGHT_4_PENALTY=0.488948
PRIMER_LEFT_4_SEQUENCE=GGAGTTTGAGTTGAGTTCAGGG
PRIMER_RIGHT_4_SEQUENCE=AGCTTGGAGAAGAGAGGGAAAG
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=194,22
PRIMER_LEFT_4_TM=60.146
PRIMER_RIGHT_4_TM=60.489
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_00566 Ps_contig_mira-and-tgicl-ass_franssen_5416
SEQUENCE=AAGCATGGTATCAACGCAGAGTGTTTGGTGGCAATGGAAAGACTGGCAGAGATTGAGAGGGCTGAGAGGAAAATTGAAACTCTTGGGAGGGAGAAAGATAATCTGGAAACTGAATTGCAGAGAGCAACGTATTCAGAGAAGGATGCACTTACCAGAGTTGCGAAGCTTGAGGAGAAAGTGCAACAGAGAGAGAAGGATTTAGAAGCACTTCTGGATAAAGATAAAACACACAGGAAAAATAACGCTGAGATTCTTGAACAACTTTTGGAAACAGAACGTGAAGCACATACACAGGCAAATAACCGGGCCGAGGCTCTCTCTCTGCAGCTACAGTCTGCACAAGCCAAAATTGACTCTCTACATCAAGAGCTGACGAAGTTTCGACTGAATGAAACATTAGACAGTAAGCTAAAAACATCCGATGGTAAGCGATTGAGGGAAGAAAATGACATTGGTGTTGATTCTGTACAAGACATGGATGCGAGCCCTAGAATTTTAAGGGGAACTAAAAGAGCTAGGAGTACATCCAGTCCTAAGTACACTCAGCCAGAAGATGGTGGTTCAATCTTCGAGGGCGGAGAGGATAATCTTAGTCAGCAAACAAATGAAGAGAATTATAAAAAGTTTACCGTCCAGAAGCTTAAGGTAGAACTGACAAAACATAACTATGGTGATCAGCTGCTTCGGTTGAAGAATCCCACCAAGAAAGATATTCTTGCTCTGTATGAGAAGTTTATTCTTCAGAAGGCATAACAGGTTTTGAAAAGATGTGTATTAGTATGCTGCATGTAATCACTCCGTCTAAGTCTCTCTAAATATCATTGATGCCGGGTTTATGAGCTGTGAAATATCGAATTAATTTTAACTTTTTTTTGTTTGTTGAGTTCTACTTGTGTTTGCTAGCAATGATTATCATTTAGGCCATACAGGGTTTTGATGGCACGATCTTTATTTGTTGATTGTTTGAGTTGTATTTAATACATCTGTACAGTAGCATTTAGTCGTATTACTCATAACTCTAACATTGTTTCCTATAGTTAGTATGAACTAATTACTAGAAAAAAGT
TARGET=314,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2291
PRIMER_LEFT_PENALTY=0.165541
PRIMER_RIGHT_PENALTY=0.063537
PRIMER_LEFT_SEQUENCE=CACACAGGAAAAATAACGCTGA
PRIMER_RIGHT_SEQUENCE=TCCCCTTAAAATTCTAGGGCTC
PRIMER_LEFT=226,22
PRIMER_RIGHT=503,22
PRIMER_LEFT_TM=60.166
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=278
PRIMER_PAIR_PENALTY_1=0.2347
PRIMER_LEFT_1_PENALTY=0.171146
PRIMER_RIGHT_1_PENALTY=0.063537
PRIMER_LEFT_1_SEQUENCE=CAGAACGTGAAGCACATACACA
PRIMER_RIGHT_1_SEQUENCE=TCCCCTTAAAATTCTAGGGCTC
PRIMER_LEFT_1=271,22
PRIMER_RIGHT_1=503,22
PRIMER_LEFT_1_TM=59.829
PRIMER_RIGHT_1_TM=59.936
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.2611
PRIMER_LEFT_2_PENALTY=0.165541
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=CACACAGGAAAAATAACGCTGA
PRIMER_RIGHT_2_SEQUENCE=ACACCAATGTCATTTTCTTCCC
PRIMER_LEFT_2=226,22
PRIMER_RIGHT_2=457,22
PRIMER_LEFT_2_TM=60.166
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.2664
PRIMER_LEFT_3_PENALTY=0.170871
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=TGAGGAGAAAGTGCAACAGAGA
PRIMER_RIGHT_3_SEQUENCE=ACACCAATGTCATTTTCTTCCC
PRIMER_LEFT_3=167,22
PRIMER_RIGHT_3=457,22
PRIMER_LEFT_3_TM=60.171
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.2818
PRIMER_LEFT_4_PENALTY=0.060824
PRIMER_RIGHT_4_PENALTY=0.220992
PRIMER_LEFT_4_SEQUENCE=AAGCTTGAGGAGAAAGTGCAAC
PRIMER_RIGHT_4_SEQUENCE=CACCAATGTCATTTTCTTCCCT
PRIMER_LEFT_4=162,22
PRIMER_RIGHT_4=456,22
PRIMER_LEFT_4_TM=60.061
PRIMER_RIGHT_4_TM=60.221
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_00567 Ps_contig_mira-and-tgicl-ass_franssen_5418
SEQUENCE=TTCATTCACTTGAATATTCATTTTAATCCATTGATTGAAGTTGCTTCACTTTGCATTAGAGTTCCAAGAGAATTTGGTGTGCTAATTTTGAGATTGTGACATAATATTTTGATGTATTATTATAGAGAAAGCACAAGAAATACAATTCCTTTCTTTCTTAGGAAAAAAATCYTTCTAACTTCATAGTATGATGAAATTGAATTTGTGGTTGTAAATGTTGATATGATCTTACCGAATATGCGACGAATATGGATTCGCTACAAAATTGAATCTAAACAATATGATATGATATGAATCTTTAGTGTGTATCTATTTGGAGTTTGGACAAT
TARGET=279,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00568 Ps_contig_mira-and-tgicl-ass_franssen_5437
SEQUENCE=AAGTACTAAAATTCAGGAATTTATATATATATATACTCAACACCAGCATTTGTTTACTTGTTACGTTTCCTGCAAATCATCAAACAACCTATGATTTTGTCTTAGTGAGGATGAGATGTYAACTTCATAACAGTTGTTCAAACTTTAAAACCTATGTATTTTGCTTAGTGAGGATAAGTATTTTGCTTAGCGAGGATGGGTATTTTAACTTCAATAAAACAGCTTGACTGTCC
TARGET=22,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00569 Ps_contig_mira-and-tgicl-ass_franssen_5442
SEQUENCE=ATCGCCGTCGATGACGTCAGAAGAATCCGTGTACTTACGTACGGCGYGCTTGGAGGCTGGGATCGGAGAAAAATGGACTCACGGTGGTGATAGGAAAGTTCTCAACCTTTTCATATGAGGACTCTCTGGGTTTGTGTTGCGTTTGCGTGTATCACCTCACGCGCGCGTCGTAACGTTCCTTTGTTTTGCTTACAATACTCATTGTGGGGGAAGGGAGAAAACGGTTTTGTAGTTGGACGAGTTTTCGTGAGTTACCGGTTCAGGATTTCTCTCGAGAGAAAAATGATGGTTCGTCGGTGATTTTTTTTCTTTCTTTTCAGTCTTTTTGATTTTATTTTATTTTCTAGCAAATATGAGACATGTAGTAAACCTGTAAGCGTGGAATATTAATATTAAATCATATGAAATAAAAATAAAGAGAAGTTTTATTTAAATTAAAAATAAACG
TARGET=329,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00570 Ps_contig_mira-and-tgicl-ass_franssen_5444
SEQUENCE=ACCTTCGTTCTCTGGGTTTCTACCCATACTCATTGCCTCACACAATCTCCTCCAAGTACATCCATGAACTGTAACCACGCCACTCTCCTTTGTCTTTCTCTCCCACCACCACCACTGAATTCAAATCGTGTCATTTTCCCAAAATGCCATTCTGCAATTCCCTTATCCAACAAAACCCTTTTGTGCAAACCGAAGCTGAAGAGAAAAACGATTCTTCTTAGAATCAATGCTTCTTCAAAGGATGATAATGCTCGATCACCAAATTGGTCTAAGTGGATCCCCACAGGCTCTTTCGCTGCTGAATTAAAGTTTTCAGATTGATTTCCAGTGCCTACCGCTACGTCCCATTGGACTAGTTTGTATCCTTCCCCCCACCTACGTTTCTTCACTCTA
TARGET=103,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2411
PRIMER_LEFT_PENALTY=0.219884
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=ACATCCATGAACTGTAACCACG
PRIMER_RIGHT_SEQUENCE=ATCGTTTTTCTCTTCAGCTTCG
PRIMER_LEFT=57,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=59.780
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.4075
PRIMER_LEFT_1_PENALTY=0.386286
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=TACCCATACTCATTGCCTCACA
PRIMER_RIGHT_1_SEQUENCE=ATCGTTTTTCTCTTCAGCTTCG
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=60.386
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.4294
PRIMER_LEFT_2_PENALTY=0.408180
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=TCTACCCATACTCATTGCCTCA
PRIMER_RIGHT_2_SEQUENCE=ATCGTTTTTCTCTTCAGCTTCG
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=59.592
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.5034
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=0.283555
PRIMER_LEFT_3_SEQUENCE=ACATCCATGAACTGTAACCACG
PRIMER_RIGHT_3_SEQUENCE=GGGTTTTGTTGGATAAGGGAAT
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=177,22
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=60.284
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=121
PRIMER_PAIR_PENALTY_4=0.5143
PRIMER_LEFT_4_PENALTY=0.493142
PRIMER_RIGHT_4_PENALTY=0.021195
PRIMER_LEFT_4_SEQUENCE=GCCACTCTCCTTTGTCTTTCTC
PRIMER_RIGHT_4_SEQUENCE=ATCGTTTTTCTCTTCAGCTTCG
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=211,22
PRIMER_LEFT_4_TM=59.507
PRIMER_RIGHT_4_TM=60.021
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_00571 Ps_contig_mira-and-tgicl-ass_franssen_5455
SEQUENCE=GGGCCTCAGTAGTTGAAATATCTCTCTCTCTCTCTCTCTCTGAGCAAGTTGTTTTCTTTAATCTTTCATATCCCAAATCTAATCTGTATCTAGCTTCTACCATTCTATACCAAAAAAATCAGAGTGAACTAACATAATTGTGAAACTAAGAAAATTAATTTAAAGGAACTGATATGAAGTGAGATTATTTTTACAAACATGGAAGGAAGGAATACAAAGATGGATAACAAAAAAGAAAAACTA
TARGET=21,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00572 Ps_contig_mira-and-tgicl-ass_franssen_5455
SEQUENCE=GGGCCTCAGTAGTTGAAATATCTCTCTCTCTCTCTCTCTCTGAGCAAGTTGTTTTCTTTAATCTTTCATATCCCAAATCTAATCTGTATCTAGCTTCTACCATTCTATACCAAAAAAATCAGAGTGAACTAACATAATTGTGAAACTAAGAAAATTAATTTAAAGGAACTGATATGAAGTGAGATTATTTTTACAAACATGGAAGGAAGGAATACAAAGATGGATAACAAAAAAGAAAAACTA
TARGET=201,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00573 Ps_contig_mira-and-tgicl-ass_franssen_5467
SEQUENCE=AGTAATAAGTTATAACAAATGATTTAAAGAAGTAAAAATTTAATGTGTGATTATTAAGCTCTGAATTGAGAGTGGAAGAGTCTGTGTGTGTGCGCGKTKGGGTTCTCTACTTCCTT
TARGET=83,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00574 Ps_contig_mira-and-tgicl-ass_franssen_5468
SEQUENCE=ACTTTTGKAACTCTTGTTCTTTTTCAATATATATAATTTCAAGTTTACACCATAAATATAATTTGAAGTTTATACAACAATATAACATATATGGTATATCCTAGCTCACACCTCACATCAACAAAATAATAACATATAAAAAAATTACTATAAAATAAATAAATGGTATTTAACTTACTAAAATAACAAAAACATCCCTAACAAGACAAGAAACACATAAAACTTGTTGTAAAGTTGTGCATACTTTAACACTGAATAAGAACCAGATTTCTCATCACTTGTTTTATCATCATCTTTGTCTTCCTTAGGATTGTTAAAATTGGAAGGAACAGTTGTGTCCGGTGCCGAGTCATCGTCACCGGAAACCGGACTAGCAGGAGAAGGAGAATCATCTGGTAGAAGACTCTTTGGCAACCCTAATCCATGAGTCACATTTATTCTGAAATGTTGTCCATTTTTGCATTGCTCGCCATCATAATCACTCGAGAAGAAATATGTTGGTCCTTCTTTCAACAAAGGAACTGCCACTGTGAGTGGAGTTACTTCACCGTTGGGTTGAGAACCTGATGACCATTGGAGTGTGTCTTTGTCCTGTGCATCATCGTAGTCACATTGTTTGTATGTTGTGAAATTGTATGTTTGAACAATAGTGTGGTTGCTGTCTGTATTGAAAATGAGAAAATCTCCCAAGCTGAATTGTTTTTTGGCAACCCATTTCTGATAATTAACAGTTGGTTTTTCAAGTTTGTCAAACCAGCCTAAAGAATCCCCCACTGTGTAATTTTTGTAAGCTCCAACAGATCCTAAGAAACATGAAAGAAAAAGACAAGAAAGAATTGAGAATAGAAGGCATGTTGATGATGATGATGATGATGATGAGTTTCTTCTATATTCCATTATTCTTGGTGTGTGTGAAATTATTGTGAATCGAAGCAAACTAAGTAAGTAGCTTTTTTATGAGTTAGTGGATGAGAATGTAGCATTCATATGCTGCCAGCTATGGTGTCTCAAGTGGTGTATATTTAAGAAGCCTTGCTTTTCTAATGAATTACTAAAATATACAATCTGTTGTGTTGGTTAGTTCC
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00575 Ps_contig_mira-and-tgicl-ass_franssen_5468
SEQUENCE=ACTTTTGKAACTCTTGTTCTTTTTCAATATATATAATTTCAAGTTTACACCATAAATATAATTTGAAGTTTATACAACAATATAACATATATGGTATATCCTAGCTCACACCTCACATCAACAAAATAATAACATATAAAAAAATTACTATAAAATAAATAAATGGTATTTAACTTACTAAAATAACAAAAACATCCCTAACAAGACAAGAAACACATAAAACTTGTTGTAAAGTTGTGCATACTTTAACACTGAATAAGAACCAGATTTCTCATCACTTGTTTTATCATCATCTTTGTCTTCCTTAGGATTGTTAAAATTGGAAGGAACAGTTGTGTCCGGTGCCGAGTCATCGTCACCGGAAACCGGACTAGCAGGAGAAGGAGAATCATCTGGTAGAAGACTCTTTGGCAACCCTAATCCATGAGTCACATTTATTCTGAAATGTTGTCCATTTTTGCATTGCTCGCCATCATAATCACTCGAGAAGAAATATGTTGGTCCTTCTTTCAACAAAGGAACTGCCACTGTGAGTGGAGTTACTTCACCGTTGGGTTGAGAACCTGATGACCATTGGAGTGTGTCTTTGTCCTGTGCATCATCGTAGTCACATTGTTTGTATGTTGTGAAATTGTATGTTTGAACAATAGTGTGGTTGCTGTCTGTATTGAAAATGAGAAAATCTCCCAAGCTGAATTGTTTTTTGGCAACCCATTTCTGATAATTAACAGTTGGTTTTTCAAGTTTGTCAAACCAGCCTAAAGAATCCCCCACTGTGTAATTTTTGTAAGCTCCAACAGATCCTAAGAAACATGAAAGAAAAAGACAAGAAAGAATTGAGAATAGAAGGCATGTTGATGATGATGATGATGATGATGAGTTTCTTCTATATTCCATTATTCTTGGTGTGTGTGAAATTATTGTGAATCGAAGCAAACTAAGTAAGTAGCTTTTTTATGAGTTAGTGGATGAGAATGTAGCATTCATATGCTGCCAGCTATGGTGTCTCAAGTGGTGTATATTTAAGAAGCCTTGCTTTTCTAATGAATTACTAAAATATACAATCTGTTGTGTTGGTTAGTTCC
TARGET=856,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00576 Ps_contig_mira-and-tgicl-ass_franssen_5477
SEQUENCE=GAAAGTGCTTTTCATTTCTGCGTTAGAATCTGAGAGAAGAAAAAGAGAAGAAGAAGAAAGAGGAGACACAGACACAAATCAATCAACTATAGGTATYTTCACGTCTGTGGCTCCTCTTAATATAAAAGCTAGTTAGTTTCTGGGTTAAAGAAAATTTAAAGGAAAAGATGGATTAATGAGAGTAATAGTTGGTAGTTGGGAGGGTGTAACTACTATCCACTATTTATTTGGTTGGTTTAATTGGTTACGAGCGKTS
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00577 Ps_contig_mira-and-tgicl-ass_franssen_5480
SEQUENCE=CGTTCAGATAGTTTTCTACATATATATATATAAATACATACAAGAATCCAYWAAAGTAAAACCTTACACGTATATAAATACCATAAGAGCTTATTTACTAACACATACTAAAAGACTCATAAAAGATATTCCTACATAATAATAATCTACCCAGAATCTCACTAGGCAGCTTCAGCAAAACCAAGTTGAAGATTTCCATAGTCAAAGATAGTGTGATATACCCTCAATAAAAATATCACCAAGAATCCATAGAGGACCCCTTGGAGGAGGAATGTACAAAACGACAAATAAAACCCAACTAAAGACAGACTTGTGTAATACCTTCTCCGGTTTTCA
TARGET=20,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00578 Ps_contig_mira-and-tgicl-ass_franssen_5482
SEQUENCE=TCCACCACCACCACAAACTCCACAAAATCACTGGATCTCATCAAAGCAAAAACGACAAAATCCACCGTCACAAAAACAGATCAACAAGAATCAAAGAAACCAAACAAGAAACAAACACCACCGACTTGGTTCTTCGACGACGAAGACACCGATTTCGTTTCAGACTTCAAAGATCTCCCAATGAGATTCCAACAAACACTAATCCCAGACCTCGAAAAAATCTCAACAACCTCAAAAGCCTATATAACAAAAACCAACCAACAAATCACAAAAAAATTTCAAACCTTACGTCGGAAACAAATACGCACCAACCATCGCAACTTTACNCTCTTGCACCTGTCGTTTTAC
TARGET=2,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00579 Ps_contig_mira-and-tgicl-ass_franssen_5538
SEQUENCE=GTTAACAAAAATAAAGCCCGACATTTCATTCATTCATTATAAAAAACCAAAGAGTTCGCATAATAGATAGATCCAGCACAAAAAACCCCTAAAAAACTAATTGCAAAAA
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00580 Ps_contig_mira-and-tgicl-ass_franssen_5545
SEQUENCE=GGWKAAATTWGACTTTTAATTGAATTATTACGGTCGTTATGGAGTAAAAACAATAATAGTTACAATAGTACATTGACAATAATATTCTCCCATAGTTCAGAAAAAAAAATTAGAATGTGGTCTCATCATGCTAAGAAATTGATATTGAGTACATAAGGACACCTTAAACAAGAAAATTGGTAGAAATCAAGGGATTGGGAAGAAATTACTTTTATATAGATATAGAAACCTGTTATTACAAACCAAGGAAAACATTAGTATTTCCAAGACATTCCTCGAGCAATCAGATTACCAATCAGATTACTCAGTATCACTTTGTATCTCGCGCTACCTATACTTAAGACACATATTTCTTCAGCTTCCTCCACTATTTCTTTTCTCAAAATGATGAATCCCAAAAATAAATCAGAGAATAATAAAAGCACCTAGAATGCTGCCCATCCTGAAGCGGCTGTTGAGCCCGGAGTGGTTGATGAGGGAAGATTCTTCTGTAATTGAGAAAGATCGGATAGAGAATCAGTAGTATTATGTCTCACGCCTTCGTGTGTCCCTTTAACACTAGATCCAGTACTGGTAGTAGAAGCAATTCGGGCAGCAACAGGATCATTTGGTGGAGGTGGAAGAGCAGATCTGATTTTCCCTGCACCACTTGGTGGAGGAGCAAGGTTCAGTACTTTTGGCTTTGGAGTTGCTATGTGAGCACCAGTTAGTCCAGCTGCTGAAAGCATGCCAGCTCCCCCAGCCGCCTTATGTTTCACATTAATCCTGATGGTTTCCCCCTCCTTGAGCCTATGATTTACAGCAGGGTGAATGTCGATCTGAGAATCCTCTGAGGCATCGGAGTTACCGGACTCCTTGTCATGCTCTCGCCGGACATACTTTTCATGATCAGATAGTGCAACATTGAAATCAAAAGCTTCGTTCCTCTCCGTGAACCCTAATCCAATGAATGCGTGCTTACCTTGACCATCTTCGATCTTGAGGACGAAGTAACGAGACGAGTCGAGAACCGGTTCGACAGAACCCTCACGCTGACCGGGGAACACGAAGCACGCGGCAAAGAGATCGCCGGAGTTTGGATCTTCTAACCGGATCTCACATCGGTCCCGACGTGATACTACCCGGAGGCGTCCCGACCAGATCTTGTCGGATTGAAGCCACTCACCGCACTTGTAGCCGCCGGAGGTTGTTCGCGGCGGGATCTTGTAGACGGCGACTTCGCGAACCACCAGTAGCGTGTGCTCGAAACCCTCTTCCTCCTCCTCTAACGACATCGTTTTGGTTGCTTTGGAGTTTGAATTTGAGTTTATTGTGAATGAATTTAAGAGACGCGTTTGTTGTTGTTGTTTAATTTGGAAATTGAAGATTGGTGTGGTGTGGGGTGTGGTGTCTTTCTCAACTCTGTCTCAAAGAAAACACCAAGGTCAACTGTTTTGGGAGATAGATTAGAAATACACACAATTATCACATTACCAATATGCTGCTCTTTGTTCCTCCATTTTCTTACCTTTCATACCTTACTTCTAAATGATTTTCCTTTCAGACCCCCGCGTACTCTGCGTTGAT
TARGET=1335,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00581 Ps_contig_mira-and-tgicl-ass_franssen_5548
SEQUENCE=TTTCAAAAGCAGTGCTCAGTTTGGCGTTGTTTTTTTGTAATTGTTTTCAAAGGCGCTGACTCTTCCTTCTGCTTGCAAAGTCTCTGCTCCCTCTCTCTCTAACTGTGGATTGTCTGTAACCCCTGCTGCTGCTCCTGGTGTAAGTCTCTCTCCAGCATCTTCTCCTACTGAAGCACCAGGTGTGGCCACTCCTGTGCTTCCATCTAGAGTACCGGCAGCCGCGCCGGGCGTCAAGAGTGCGGCAGCATCAGCATTGCTCCCTATTTCAGCAGGATCCATTCTTGTTTGTCTCTTATTTCCATTCTCAAGCATTTGAGTAGCCATGTTTAGTTTCATTGAGAGTAGGAAGGTTATGTCATGAGGGATTATTTTGAGTTTTGAAGGTTTTTTTTGGAGTATTATTACCATTAGAGCTATTTTCTTTGTGTGTGCAATTTAAACCTATTCATGTATATTTATTTATTTATGAGTAGTGTAATCTTGCTATTTTTTATTTTGTTGTACG
TARGET=91,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2546
PRIMER_LEFT_PENALTY=0.185055
PRIMER_RIGHT_PENALTY=0.069526
PRIMER_LEFT_SEQUENCE=CTTCCTTCTGCTTGCAAAGTCT
PRIMER_RIGHT_SEQUENCE=GAATGGATCCTGCTGAAATAGG
PRIMER_LEFT=61,22
PRIMER_RIGHT=280,22
PRIMER_LEFT_TM=60.185
PRIMER_RIGHT_TM=59.930
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.3362
PRIMER_LEFT_1_PENALTY=0.185055
PRIMER_RIGHT_1_PENALTY=0.151106
PRIMER_LEFT_1_SEQUENCE=CTTCCTTCTGCTTGCAAAGTCT
PRIMER_RIGHT_1_SEQUENCE=CCGGTACTCTAGATGGAAGCAC
PRIMER_LEFT_1=61,22
PRIMER_RIGHT_1=214,22
PRIMER_LEFT_1_TM=60.185
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=154
PRIMER_PAIR_PENALTY_2=0.4598
PRIMER_LEFT_2_PENALTY=0.185055
PRIMER_RIGHT_2_PENALTY=0.274768
PRIMER_LEFT_2_SEQUENCE=CTTCCTTCTGCTTGCAAAGTCT
PRIMER_RIGHT_2_SEQUENCE=CTGCCGGTACTCTAGATGGAAG
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=217,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=60.275
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.4627
PRIMER_LEFT_3_PENALTY=0.185055
PRIMER_RIGHT_3_PENALTY=0.277599
PRIMER_LEFT_3_SEQUENCE=CTTCCTTCTGCTTGCAAAGTCT
PRIMER_RIGHT_3_SEQUENCE=AGGAGAAGATGCTGGAGAGAGA
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=165,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=59.722
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.4870
PRIMER_LEFT_4_PENALTY=0.185055
PRIMER_RIGHT_4_PENALTY=0.301926
PRIMER_LEFT_4_SEQUENCE=CTTCCTTCTGCTTGCAAAGTCT
PRIMER_RIGHT_4_SEQUENCE=ACCTGGTGCTTCAGTAGGAGAA
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=180,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=60.302
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_00582 Ps_contig_mira-and-tgicl-ass_franssen_5548
SEQUENCE=TTTCAAAAGCAGTGCTCAGTTTGGCGTTGTTTTTTTGTAATTGTTTTCAAAGGCGCTGACTCTTCCTTCTGCTTGCAAAGTCTCTGCTCCCTCTCTCTCTAACTGTGGATTGTCTGTAACCCCTGCTGCTGCTCCTGGTGTAAGTCTCTCTCCAGCATCTTCTCCTACTGAAGCACCAGGTGTGGCCACTCCTGTGCTTCCATCTAGAGTACCGGCAGCCGCGCCGGGCGTCAAGAGTGCGGCAGCATCAGCATTGCTCCCTATTTCAGCAGGATCCATTCTTGTTTGTCTCTTATTTCCATTCTCAAGCATTTGAGTAGCCATGTTTAGTTTCATTGAGAGTAGGAAGGTTATGTCATGAGGGATTATTTTGAGTTTTGAAGGTTTTTTTTGGAGTATTATTACCATTAGAGCTATTTTCTTTGTGTGTGCAATTTAAACCTATTCATGTATATTTATTTATTTATGAGTAGTGTAATCTTGCTATTTTTTATTTTGTTGTACG
TARGET=453,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00583 Ps_contig_mira-and-tgicl-ass_franssen_5573
SEQUENCE=ATCCCAAACCCCAACTTTAGTCTCATGAGGTTAGGATATCTTCTACATTAATGAACAACTGCTCCCAGACTCCTTGGCAATAGCTTTTGCAAGCATGGTTTTCCCAGTGCCAGGGGGTCCATACAACAGAACCCCCTTCTGTGGTCCAAGAAGCTTGCCATGCGAAAACAAGTCGGGCCGTTTCAAGGGTAGAATAACAAGCTCAAACAAAGCTTGTTTAATAGTGTCCAGTCCCCCAATGGAGTCAAATTCCACATCAATATGGTCAGGGTTTATAACATCACAGGCTATTACATCCTCATAGGGATTGGTTTGAATGAGGGGACGACCTAGACGCTTGGCAATCTGTTTCTTTTGTTCAAGAGCTTTCTTGGTTGATTCACGGTTGGGGTCAAGGTATCGGAGTCCGGTGACCAATACCCAGTAACTCAGCGCCGCCCTAGCTGCGTACAACAACAGCTCTTGCAGAATCTTGGTATTGGCTGAACCGGTCATTCTCTAAAGTTCAAAGTATAACCCTAAAACTTAGGGTAAAAAAATTATCTTTTCTTCTTCTTCGAAGTATAGCTCCTAAAATTCAAAAGTATTATCAGCTCTCTGTGAGAGAGACCACCGAGATTTTAGGATGAAATGAAATACGAGGTCGTGAAATAAAAGGAAAAATCGAMG
TARGET=547,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00584 Ps_contig_mira-and-tgicl-ass_franssen_5575
SEQUENCE=AATTTGGACATGATCTGACACTGAATGAAGCAAGCAAGCGATTCCAGGCTTACTTAGAAGACAGAAATACTCCACTTCTTCCACCTGATATAAGAAGGGCAGTTTATGTGGCTGTAGTAAAACAGGCAACCAAATCTATCCGGTCGGGTTATGAATCACTTCTGAAAGTATACAGAGAAACTGACCTAAGTGAGGAGAAAACACGTATTCTGGGCTCACTGTCTTCTTCATCTGATCCAGACCTAATTCTTGAAGTTTA
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9764
PRIMER_LEFT_PENALTY=0.861164
PRIMER_RIGHT_PENALTY=0.115189
PRIMER_LEFT_SEQUENCE=TTGGACATGATCTGACACTGAA
PRIMER_RIGHT_SEQUENCE=AGATGAAGAAGACAGTGAGCCC
PRIMER_LEFT=3,22
PRIMER_RIGHT=232,22
PRIMER_LEFT_TM=59.139
PRIMER_RIGHT_TM=59.885
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.9764
PRIMER_LEFT_1_PENALTY=0.861164
PRIMER_RIGHT_1_PENALTY=0.115189
PRIMER_LEFT_1_SEQUENCE=TTTGGACATGATCTGACACTGA
PRIMER_RIGHT_1_SEQUENCE=AGATGAAGAAGACAGTGAGCCC
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=59.139
PRIMER_RIGHT_1_TM=59.885
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=1.1265
PRIMER_LEFT_2_PENALTY=1.011297
PRIMER_RIGHT_2_PENALTY=0.115189
PRIMER_LEFT_2_SEQUENCE=GGACATGATCTGACACTGAATGA
PRIMER_RIGHT_2_SEQUENCE=AGATGAAGAAGACAGTGAGCCC
PRIMER_LEFT_2=5,23
PRIMER_RIGHT_2=232,22
PRIMER_LEFT_2_TM=59.989
PRIMER_RIGHT_2_TM=59.885
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=228
PRIMER_PAIR_PENALTY_3=1.2922
PRIMER_LEFT_3_PENALTY=0.861164
PRIMER_RIGHT_3_PENALTY=0.431057
PRIMER_LEFT_3_SEQUENCE=TTGGACATGATCTGACACTGAA
PRIMER_RIGHT_3_SEQUENCE=TCAGAAGTGATTCATAACCCGA
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=164,22
PRIMER_LEFT_3_TM=59.139
PRIMER_RIGHT_3_TM=59.569
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=1.2922
PRIMER_LEFT_4_PENALTY=0.861164
PRIMER_RIGHT_4_PENALTY=0.431057
PRIMER_LEFT_4_SEQUENCE=TTTGGACATGATCTGACACTGA
PRIMER_RIGHT_4_SEQUENCE=TCAGAAGTGATTCATAACCCGA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=164,22
PRIMER_LEFT_4_TM=59.139
PRIMER_RIGHT_4_TM=59.569
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_00585 Ps_contig_mira-and-tgicl-ass_franssen_5628
SEQUENCE=GATTGTTAACAATGTTGGGTCTTTCASAAGGTACATATCATCATTACATTCTTTGCCTTCTCACACATTCTCTCTCTCTCTCTCTCACMTTCTTTCTCTTCTGTCTACTTTTMGAACACAGCAACAAACTAAAATWACCAAAACCTTAGTTTACGGTTTAATCCGCTT
TARGET=69,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00586 Ps_contig_mira-and-tgicl-ass_franssen_5630
SEQUENCE=CCGTTCTSACACATTCTCTCTCTCTCTCTCTCCAACTCTTTTCTCTTCTGTCACTTTTGAACTACTAGCACAACAAAAATTACAAAACCTATTCGGTTTAATCGCTCTTATT
TARGET=15,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00587 Ps_contig_mira-and-tgicl-ass_franssen_5642
SEQUENCE=TCACMAAAACTTACTCAAATCGATGAAGAACAATCACTCTCTCCGGATTCTGTTATCAACACTTGGGAACTCATGGATGGTTTGGATGAACATGAAGATTCTAATGTTCATAAAGCTTTAATCTTTGATAACCCAGTGAGTTTTTCAGATAAACACAGTTCATGCAGGTACACAGCCTTTGATGGGTCTGCAAAAAAGAAACTTCTTGATTCATTTGAATCACTGAAAGCTTCGGAAGCTGTAATGGAAGAAAAAAATTCCAAATCTTTTGTGAAGAAACCACTTTGGAAGCATTTATCAGAAGAAGCTTTGTTAGCTAAGTTGGATCCAAGTGTTGCTTGGAGTTACAGAAGAGCATTATCTTCAAGACAACTAGGTTGCAACAACAACAAAAACCGGTTAAGATGTGTAAGATCAATGGAATCAAGTCCTATGAACCCTTCTTGTTCTTCTTTGTTTGGTAAAAGTTTGTGTCTTTTACCAGGAACAGAAGACAGAATTGTTGTTTACTGCACAAGTTTGCGAGGGATTCGAAAGACTTACGAGGATTGTTGTTCAGTGAGGATGATTCTGAGAGGATTTCGAGTTGCGGTCGATGAAAGAGACATTTCGATGGATTCATCTTACAGAAAGGAGTTACAGAATGCACTTGGTGGAAAATCAGTAGTGACATTGCCACAGGTTTTTATCAGAGGGAAACATGTTGGAAATGCGGAGGATTTGAAACAATTGAATGAATCCGGTGAATTGGCGAAACTGTTGAGTGGTTTTCCAACTCAAGATCTTTGGTTTGTTTGTGATAAATGTGGTGATGCAAGGTTTGTGCCTTGTGATAATTGCAATGGTAGCAGGAAAGTGTTTGAGGAAGAACAAGGGAAGTTGAAAAGGTGTGTTCATTGTAATGAGAATGGATTGATAAGATGCACAAGCTGTTGTTCATGAATCAGTGATTACCAAAATTACTGATTGATTGATGTTATCTTTTTATTGTTGTTTTTGTTTTTCTTGGGTTGAGTTTGTTATTCTGATAAATTTGGCAGCTACTATTAATGAATATGCATGTTTCCATTTAGATTCGC
TARGET=381,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00588 Ps_contig_mira-and-tgicl-ass_franssen_5642
SEQUENCE=TCACMAAAACTTACTCAAATCGATGAAGAACAATCACTCTCTCCGGATTCTGTTATCAACACTTGGGAACTCATGGATGGTTTGGATGAACATGAAGATTCTAATGTTCATAAAGCTTTAATCTTTGATAACCCAGTGAGTTTTTCAGATAAACACAGTTCATGCAGGTACACAGCCTTTGATGGGTCTGCAAAAAAGAAACTTCTTGATTCATTTGAATCACTGAAAGCTTCGGAAGCTGTAATGGAAGAAAAAAATTCCAAATCTTTTGTGAAGAAACCACTTTGGAAGCATTTATCAGAAGAAGCTTTGTTAGCTAAGTTGGATCCAAGTGTTGCTTGGAGTTACAGAAGAGCATTATCTTCAAGACAACTAGGTTGCAACAACAACAAAAACCGGTTAAGATGTGTAAGATCAATGGAATCAAGTCCTATGAACCCTTCTTGTTCTTCTTTGTTTGGTAAAAGTTTGTGTCTTTTACCAGGAACAGAAGACAGAATTGTTGTTTACTGCACAAGTTTGCGAGGGATTCGAAAGACTTACGAGGATTGTTGTTCAGTGAGGATGATTCTGAGAGGATTTCGAGTTGCGGTCGATGAAAGAGACATTTCGATGGATTCATCTTACAGAAAGGAGTTACAGAATGCACTTGGTGGAAAATCAGTAGTGACATTGCCACAGGTTTTTATCAGAGGGAAACATGTTGGAAATGCGGAGGATTTGAAACAATTGAATGAATCCGGTGAATTGGCGAAACTGTTGAGTGGTTTTCCAACTCAAGATCTTTGGTTTGTTTGTGATAAATGTGGTGATGCAAGGTTTGTGCCTTGTGATAATTGCAATGGTAGCAGGAAAGTGTTTGAGGAAGAACAAGGGAAGTTGAAAAGGTGTGTTCATTGTAATGAGAATGGATTGATAAGATGCACAAGCTGTTGTTCATGAATCAGTGATTACCAAAATTACTGATTGATTGATGTTATCTTTTTATTGTTGTTTTTGTTTTTCTTGGGTTGAGTTTGTTATTCTGATAAATTTGGCAGCTACTATTAATGAATATGCATGTTTCCATTTAGATTCGC
TARGET=964,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00589 Ps_contig_mira-and-tgicl-ass_franssen_5679
SEQUENCE=CGTTTATCCTTCCTTTTCAACCAATAAGGTGAARATGACRTCGGCTCTTTCATCTTATGCTAAGTATTTCAATTACAGTAGTTTTTCTAGTTGTCGTAATCATTCAGTAATGCATTTATAATCCCTTATTCCTTTAATATGTAGTTATATTTTTGACAGTGCTATAGTTTTGACAATGTTATAGTTCTTCTGTTAGTTAGAATTACATCAGCATGGGTGATTCAGTTTGTTTAAAAATTTTGTTTCTGTATTACTCTAATTTCATATTAATATTCTGGAGTGGAGGCATGTTAWTTTTTAAATTTCTTGTTTGCATAATTATCTATCATTATTATTATTATTTGTGGAGTTAGTTGTACTATCGGTTCTTTGATAACACTCATTCCTACTCTTGTATGTTTATTAAATTAATTATTTTGTAGT
TARGET=328,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00590 Ps_contig_mira-and-tgicl-ass_franssen_5697
SEQUENCE=CAATCCTGCAACTTGATTGTTTGAGCTGGACTCAGACACTGCCTTCGTTACGAATTTGTCGAATTTTACGTTACATGATGGTGAAACCTGCTGGAAGGGTTTCACCGATGCAACATGACAAACACTTCCTAGTTTATCACATGAACCAACCTTAACCCTGCCTCCAAGAATCACAGAACCTAACTTCTGTGATGAAGTAACCCTAGGTCTTGACATTGCAACCGAAATATTAGAAACCGATGTTGTTGTAGCCATTTCGAATTATCCTAATCTGAACCTAAATCTTTTGGCAACGAAGGGATGAATGTGGAGAGAGAGAAATGAGAGAGTTTGAAACGGTATGTATGAGATGAAACTGAGGGTAGAAAATGGAGGAGTGGAATAAACGAAAAACCTTGGAAGTGTGTGTCGATGGAAACAATGTTGTTGTGTTTTAATGTGAAGTTGTTTAGAGTCC
TARGET=310,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0605
PRIMER_LEFT_PENALTY=0.003602
PRIMER_RIGHT_PENALTY=0.056850
PRIMER_LEFT_SEQUENCE=GAACCTAAATCTTTTGGCAACG
PRIMER_RIGHT_SEQUENCE=ACACACTTCCAAGGTTTTTCGT
PRIMER_LEFT=273,22
PRIMER_RIGHT=406,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.943
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.1682
PRIMER_LEFT_1_PENALTY=0.003602
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=GAACCTAAATCTTTTGGCAACG
PRIMER_RIGHT_1_SEQUENCE=TCGTTTATTCCACTCCTCCATT
PRIMER_LEFT_1=273,22
PRIMER_RIGHT_1=388,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=116
PRIMER_PAIR_PENALTY_2=0.1682
PRIMER_LEFT_2_PENALTY=0.003602
PRIMER_RIGHT_2_PENALTY=0.164639
PRIMER_LEFT_2_SEQUENCE=GAACCTAAATCTTTTGGCAACG
PRIMER_RIGHT_2_SEQUENCE=TTCGTTTATTCCACTCCTCCAT
PRIMER_LEFT_2=273,22
PRIMER_RIGHT_2=389,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=59.835
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=0.1781
PRIMER_LEFT_3_PENALTY=0.121299
PRIMER_RIGHT_3_PENALTY=0.056850
PRIMER_LEFT_3_SEQUENCE=CTAAATCTTTTGGCAACGAAGG
PRIMER_RIGHT_3_SEQUENCE=ACACACTTCCAAGGTTTTTCGT
PRIMER_LEFT_3=277,22
PRIMER_RIGHT_3=406,22
PRIMER_LEFT_3_TM=60.121
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=130
PRIMER_PAIR_PENALTY_4=0.1859
PRIMER_LEFT_4_PENALTY=0.129072
PRIMER_RIGHT_4_PENALTY=0.056850
PRIMER_LEFT_4_SEQUENCE=GCAACCGAAATATTAGAAACCG
PRIMER_RIGHT_4_SEQUENCE=ACACACTTCCAAGGTTTTTCGT
PRIMER_LEFT_4=217,22
PRIMER_RIGHT_4=406,22
PRIMER_LEFT_4_TM=59.871
PRIMER_RIGHT_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_00591 Ps_contig_mira-and-tgicl-ass_franssen_5703
SEQUENCE=GGCATTTGTAAGGCGGTGCAATCTACAAAAATAGCTTTCTGGGGTGAAATTCATTCCCCGTGACCAGGTAGAAGATCGGGACTTAGATAAGGATTCTGTCTCCAAGGAAAGAAAGAAATCAGACAGTAGGAGGAAAGAGGATAAGAGCCCTAGGAATAGCTCTTCTGATGAGGAGGAGCTTGATAGAATAAAAGGTTCTAGAAAGAAGAAGTTGTATTCATCGGATGAAGATTCTTCATTTTATACAATCGACAGTGAAAGTGATCGGGATGATAAGAAAAGGAAAAGTAAAGGAAAGAAGAAAAAGGACAAGAGTTATAGGAATATCTCTTCTGATGAGGATGCGTTGGATGAGATGAAAAAGGGATCTAGAAAGAAGCGGTACTCATCTGATGAGAATTCTTCATTTTATACAAGCGAAAGTGAAAGCGACCGGGATGATAAGAAAAGGAGAAGGAAAGGAAAGAAGAAAAAGGGTAGGAGTAGAGAGAACAAGATCAAAGGAGAAGCTGCCGACATTGCAGCCAATTCTACAAGTAAGAAGGATATTGCTAGAAAAGAAATGGGGTTGGATTGG
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0793
PRIMER_LEFT_PENALTY=0.390220
PRIMER_RIGHT_PENALTY=0.689105
PRIMER_LEFT_SEQUENCE=CCAGGTAGAAGATCGGGACTTA
PRIMER_RIGHT_SEQUENCE=CGATCACTTTCACTGTCGATTG
PRIMER_LEFT=63,22
PRIMER_RIGHT=266,22
PRIMER_LEFT_TM=59.610
PRIMER_RIGHT_TM=60.689
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=1.0966
PRIMER_LEFT_1_PENALTY=0.460533
PRIMER_RIGHT_1_PENALTY=0.636023
PRIMER_LEFT_1_SEQUENCE=ACAAAAATAGCTTTCTGGGGTG
PRIMER_RIGHT_1_SEQUENCE=GGCTCTTATCCTCTTTCCTCCT
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=148,22
PRIMER_LEFT_1_TM=59.539
PRIMER_RIGHT_1_TM=59.364
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=1.0966
PRIMER_LEFT_2_PENALTY=0.460533
PRIMER_RIGHT_2_PENALTY=0.636023
PRIMER_LEFT_2_SEQUENCE=ACAAAAATAGCTTTCTGGGGTG
PRIMER_RIGHT_2_SEQUENCE=AGGGCTCTTATCCTCTTTCCTC
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=150,22
PRIMER_LEFT_2_TM=59.539
PRIMER_RIGHT_2_TM=59.364
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=127
PRIMER_PAIR_PENALTY_3=1.0982
PRIMER_LEFT_3_PENALTY=0.462203
PRIMER_RIGHT_3_PENALTY=0.636023
PRIMER_LEFT_3_SEQUENCE=CAAAAATAGCTTTCTGGGGTGA
PRIMER_RIGHT_3_SEQUENCE=GGCTCTTATCCTCTTTCCTCCT
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=148,22
PRIMER_LEFT_3_TM=60.462
PRIMER_RIGHT_3_TM=59.364
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=1.0982
PRIMER_LEFT_4_PENALTY=0.462203
PRIMER_RIGHT_4_PENALTY=0.636023
PRIMER_LEFT_4_SEQUENCE=CAAAAATAGCTTTCTGGGGTGA
PRIMER_RIGHT_4_SEQUENCE=AGGGCTCTTATCCTCTTTCCTC
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=150,22
PRIMER_LEFT_4_TM=60.462
PRIMER_RIGHT_4_TM=59.364
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_00592 Ps_contig_mira-and-tgicl-ass_franssen_5705
SEQUENCE=ATAAGGATTCTGTCTCCAAGGAAAGAAAGAAATCAGACAGTAGGAGGAAARAGGATAGGAGCCCTAGGAATAGCTCTTCTGATGAGCAGGAGCTTGATAGAATAAAAGGTTCTAGAAAGAAGAAGTGGTATTCATCGGATGAAGATTCTTCATTTTACAGTGAAAGTGATCGGGATGATAAGAAAAGGAAAAGGAAAGGAAAGAAAGAAAAAGGGACAAGAGTTATAGGAATATCTCTTCTGATGAGGATGCGTTGGATGAGATGAAAAAGGGATCTAGAAAGAAGCGGTACTCATCTGATGAGAATTCTTCTATTTTATACAAGCGAAAGTGAAAGCGACCGGGATGAATAAAGAAAAAGGAAAAGGAAAAAAAAACGGG
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00593 Ps_contig_mira-and-tgicl-ass_franssen_5705
SEQUENCE=ATAAGGATTCTGTCTCCAAGGAAAGAAAGAAATCAGACAGTAGGAGGAAARAGGATAGGAGCCCTAGGAATAGCTCTTCTGATGAGCAGGAGCTTGATAGAATAAAAGGTTCTAGAAAGAAGAAGTGGTATTCATCGGATGAAGATTCTTCATTTTACAGTGAAAGTGATCGGGATGATAAGAAAAGGAAAAGGAAAGGAAAGAAAGAAAAAGGGACAAGAGTTATAGGAATATCTCTTCTGATGAGGATGCGTTGGATGAGATGAAAAAGGGATCTAGAAAGAAGCGGTACTCATCTGATGAGAATTCTTCTATTTTATACAAGCGAAAGTGAAAGCGACCGGGATGAATAAAGAAAAAGGAAAAGGAAAAAAAAACGGG
TARGET=199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00594 Ps_contig_mira-and-tgicl-ass_franssen_5706
SEQUENCE=TATGTGTTTCTCTTCACAACATGACACCTTTTACTTGCTCCCTACCTCTTCCAGAAACATAGGAACCTTAGATTAACTCACCNCAAAGAAGGAGAACACAATTGAAAGAAGCAAAGAAAGGAAAAAGATGATTATAGTGAAAGAAGAGATTAAGATGAAGAACTTGAAATTGTATATGGAGAATCAAACCATCATTGAGGAGAATGAAAAGTTGAGGAAACAAGCTATGCTTTTGCATAAAGAGAATCAAGACCTTATGTCTCAGCTTCAAATTAAGCTTTCACTACAAAATATCAATACCAACAATAACTAGTTTTTCAATTAATTAATTTTCTAGCAATAAGTTTAGAGTTATATAATAAAGATATTGAATAGGTAATATTTTAATTGTAACATTGCATAGAATATGATATTGTGCAATATTGTTGAAATGGGTCAAATGTTTGACTTCTTGATGGTATGTTTAGAGAAGGGAAAATAGAAAGCAAAAATGTCTTGGTTTTACTTATATTATGAGTGTAATTGTATTAAAGTCAAGCAAGTAGTTAAAAATTAAAAAGTTTTTTTTTG
TARGET=320,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.5826
PRIMER_LEFT_PENALTY=1.496015
PRIMER_RIGHT_PENALTY=3.086550
PRIMER_LEFT_SEQUENCE=GAATCAAACCATCATTGAGGAG
PRIMER_RIGHT_SEQUENCE=TCCCTTCTCTAAACATACCATCAAG
PRIMER_LEFT=180,22
PRIMER_RIGHT=474,25
PRIMER_LEFT_TM=58.504
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=4.5826
PRIMER_LEFT_1_PENALTY=1.496015
PRIMER_RIGHT_1_PENALTY=3.086550
PRIMER_LEFT_1_SEQUENCE=GGAGAATCAAACCATCATTGAG
PRIMER_RIGHT_1_SEQUENCE=TCCCTTCTCTAAACATACCATCAAG
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=474,25
PRIMER_LEFT_1_TM=58.504
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=4.5826
PRIMER_LEFT_2_PENALTY=1.496015
PRIMER_RIGHT_2_PENALTY=3.086550
PRIMER_LEFT_2_SEQUENCE=GAATCAAACCATCATTGAGGAG
PRIMER_RIGHT_2_SEQUENCE=CCCTTCTCTAAACATACCATCAAGA
PRIMER_LEFT_2=180,22
PRIMER_RIGHT_2=473,25
PRIMER_LEFT_2_TM=58.504
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=294
PRIMER_PAIR_PENALTY_3=4.5826
PRIMER_LEFT_3_PENALTY=1.496015
PRIMER_RIGHT_3_PENALTY=3.086550
PRIMER_LEFT_3_SEQUENCE=GGAGAATCAAACCATCATTGAG
PRIMER_RIGHT_3_SEQUENCE=CCCTTCTCTAAACATACCATCAAGA
PRIMER_LEFT_3=177,22
PRIMER_RIGHT_3=473,25
PRIMER_LEFT_3_TM=58.504
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=4.6512
PRIMER_LEFT_4_PENALTY=1.564634
PRIMER_RIGHT_4_PENALTY=3.086550
PRIMER_LEFT_4_SEQUENCE=TGAGGAGAATGAAAAGTTGAGG
PRIMER_RIGHT_4_SEQUENCE=TCCCTTCTCTAAACATACCATCAAG
PRIMER_LEFT_4=195,22
PRIMER_RIGHT_4=474,25
PRIMER_LEFT_4_TM=58.435
PRIMER_RIGHT_4_TM=59.913
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_00595 Ps_contig_mira-and-tgicl-ass_franssen_5738
SEQUENCE=TCCTCCTAGATCTCTCCTCATCACTTCCTTCAATGGCTACTTCTTCTTCTTCTTCTTCACCTATTAGAAGATTCTATCTTATTCTTTACAATTGGACTGTTTTATTTGG
TARGET=40,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00596 Ps_contig_mira-and-tgicl-ass_franssen_5741
SEQUENCE=CKTAGCAAAAACTTTAGGATATATCTTTCAAGCCACACTTACATAYATAGTAGAGGAAATTTTTTATTAATTGAAAGAAATGAACAGCTCAGTTGCAATTACAAATATCGCGTCTATAGTCAGGAAACCATATACTGCTTAACTATTAGTATTATTTAGGTCCAAATAAACGATTCCCTGTCGTATATATATAGCTCTCTCTTTTTACATACTAGTATGTTGTAGTTGGTGGAAGCTAGATGAATCCCCAAAATGGAGAACCTTTTTGTACTGAATCCACGGCTTGACCACCACGACGGAGGTGAACATTCTGCGAACACCATTATGATCAACGGCTGAAATATTGAGATAATACTTCATCCCTGACACCACTTGCTTCTTTGCCTCCACCACCTCCACAAACTTCAACGCCTCGCCTCCGCCGTTGCTTTTTTTGTAGTTGTACTCCTCTACCGCGAACTTCCCAAGCTCCTGCACCTCCATGTTTGTCCTCACGTCAGTAATCTCCGTCTTCGCTCCAACTATCAGCCGGCCACACGACGCCGTTGATAGAATGCAGAGCAAGCTGGTTATGAGGATTGCAATATTCACAGGAACAGCCATCATAAAACCACAAAAGAAATGATTAACTTGTGTGTAGGGTAGGGTGTATAAGAAGGAGAATGAATGAAAAGATATCCCCGACTCTTGCGTTGGATACCACTGCTT
TARGET=184,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00597 Ps_contig_mira-and-tgicl-ass_franssen_5742
SEQUENCE=CGGGGGTTTATTTAATTTTTTAAAGTTAGGAGGCACACACACCAGAAAGGTCAACGATCGAAAGAGGAGGCGGTGCCACTGGCAAACTGACAGAACTCGAATCATGATAGGCATATCAGTTACCGGTATCATGTACACACTCTCCCCAACAATCTCTTCATTCACGCGCTTCTCACCCACTTTTTTCACTTCCAACTTTCGTCTTCGAGCTTCTTCTTCTTACAATACTTCTTCAACCACATTTCTTGACACCAATCCAACACTGGATTCCGTTGTAG
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1715
PRIMER_LEFT_PENALTY=0.046801
PRIMER_RIGHT_PENALTY=0.124655
PRIMER_LEFT_SEQUENCE=CACTGGCAAACTGACAGAACTC
PRIMER_RIGHT_SEQUENCE=GTGTTGGATTGGTGTCAAGAAA
PRIMER_LEFT=76,22
PRIMER_RIGHT=262,22
PRIMER_LEFT_TM=59.953
PRIMER_RIGHT_TM=59.875
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.1715
PRIMER_LEFT_1_PENALTY=0.046801
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=CACTGGCAAACTGACAGAACTC
PRIMER_RIGHT_1_SEQUENCE=GGTGTCAAGAAATGTGGTTGAA
PRIMER_LEFT_1=76,22
PRIMER_RIGHT_1=252,22
PRIMER_LEFT_1_TM=59.953
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.1845
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=CTCCCCAACAATCTCTTCATTC
PRIMER_RIGHT_2_SEQUENCE=GTGTTGGATTGGTGTCAAGAAA
PRIMER_LEFT_2=141,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=0.1845
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=TCTCCCCAACAATCTCTTCATT
PRIMER_RIGHT_3_SEQUENCE=GTGTTGGATTGGTGTCAAGAAA
PRIMER_LEFT_3=140,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=123
PRIMER_PAIR_PENALTY_4=0.1845
PRIMER_LEFT_4_PENALTY=0.059875
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=CTCCCCAACAATCTCTTCATTC
PRIMER_RIGHT_4_SEQUENCE=GGTGTCAAGAAATGTGGTTGAA
PRIMER_LEFT_4=141,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=59.940
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=112
=
PRIMER_SEQUENCE_ID=SSR_00598 Ps_contig_mira-and-tgicl-ass_franssen_5745
SEQUENCE=GGACCCAAATATCAATATCATAACAAAATCTCTCTCTCGAATTACAGAACAAGTGTTGTGAGGGAACAGTGGATGGGGTGCTGCTAGTTTCTCACTTCATTCCAAAAATGGTGCACAGACTCTCTCTAATGGCTTCTCACGTCTACCCTTCACCCTTGCCCTTCCCCACATCACACATCAAGGACTGTCAAACTTTTGCACCTTCTTTCTTTTGACAAAAACCAGACATGCTCAATTATCAGTACTTCCTTTTACGTTAAGGGCGAGTCCATTTGTAGAGTCGATGAAAACCCAAAATAAATGGTTTGATGAAAGT
TARGET=29,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00599 Ps_contig_mira-and-tgicl-ass_franssen_5752
SEQUENCE=CGAACAANACAGTTNAGAGAGWACATGATCGGTGTTCCTAACGGTGCCCSTTTGGGTTGCCGGTAGGGGTTGGCAGCGGATGAACAGTAGATGAGGTAAGCCAAGACTTCTCTGTCTTCGGTTGAGAGAGCAGCTGCTAGGGTGAGGATGGTTGCCGGAAGGAAGGAAAGTTGGTCGGTGATAACCGGTGGGGTTGGATGAACCGTCGCTTTATGGTAGAGCTTCTTCATGGTTGAACAAAAAGATGAAAAAAGTC
TARGET=157,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00600 Ps_contig_mira-and-tgicl-ass_franssen_5756
SEQUENCE=TTAAAAATAAAACCAAGTTCATAATACTCCCAACCTAAAAAGTAAAAAGTTACATTCATAAATCATCATAATTATACACTAGAGTACTTCTAAAGTATATCAAGATTTTCCAGATGCTCAATTTTTTCATGCATGAAACTGTAACCGTCCTCCGCAACGAGTCTTGACAACCCGGCCACAGAATATCAAAGAAATAAATAAATCACCAAAAATTTACTAGGACAGTCTTCATGCATCTTCTGTTTTTTTATCTGATCTTCCAATTGACACTTATCACTGAACTAGTTTCCTGCTTCACCTTCCAAGTTTGAACCTTTTTGTGG
TARGET=192,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8899
PRIMER_LEFT_PENALTY=0.428205
PRIMER_RIGHT_PENALTY=0.461713
PRIMER_LEFT_SEQUENCE=CATGCATGAAACTGTAACCGTC
PRIMER_RIGHT_SEQUENCE=AAAGGTTCAAACTTGGAAGGTG
PRIMER_LEFT=127,22
PRIMER_RIGHT=316,22
PRIMER_LEFT_TM=60.428
PRIMER_RIGHT_TM=59.538
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=1.1791
PRIMER_LEFT_1_PENALTY=0.428205
PRIMER_RIGHT_1_PENALTY=0.750914
PRIMER_LEFT_1_SEQUENCE=CATGCATGAAACTGTAACCGTC
PRIMER_RIGHT_1_SEQUENCE=CCACAAAAAGGTTCAAACTTGG
PRIMER_LEFT_1=127,22
PRIMER_RIGHT_1=322,22
PRIMER_LEFT_1_TM=60.428
PRIMER_RIGHT_1_TM=60.751
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=1.2344
PRIMER_LEFT_2_PENALTY=0.772692
PRIMER_RIGHT_2_PENALTY=0.461713
PRIMER_LEFT_2_SEQUENCE=CATGAAACTGTAACCGTCCTCC
PRIMER_RIGHT_2_SEQUENCE=AAAGGTTCAAACTTGGAAGGTG
PRIMER_LEFT_2=131,22
PRIMER_RIGHT_2=316,22
PRIMER_LEFT_2_TM=60.773
PRIMER_RIGHT_2_TM=59.538
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=1.3753
PRIMER_LEFT_3_PENALTY=0.913575
PRIMER_RIGHT_3_PENALTY=0.461713
PRIMER_LEFT_3_SEQUENCE=GCATGAAACTGTAACCGTCCTC
PRIMER_RIGHT_3_SEQUENCE=AAAGGTTCAAACTTGGAAGGTG
PRIMER_LEFT_3=130,22
PRIMER_RIGHT_3=316,22
PRIMER_LEFT_3_TM=60.914
PRIMER_RIGHT_3_TM=59.538
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=1.5236
PRIMER_LEFT_4_PENALTY=0.772692
PRIMER_RIGHT_4_PENALTY=0.750914
PRIMER_LEFT_4_SEQUENCE=CATGAAACTGTAACCGTCCTCC
PRIMER_RIGHT_4_SEQUENCE=CCACAAAAAGGTTCAAACTTGG
PRIMER_LEFT_4=131,22
PRIMER_RIGHT_4=322,22
PRIMER_LEFT_4_TM=60.773
PRIMER_RIGHT_4_TM=60.751
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_00601 Ps_contig_mira-and-tgicl-ass_franssen_5784
SEQUENCE=AAAGACAAGTAGGTAATCAACGCAGAGTTTAGGTTGTTTCATGGAGCAAATGAGGTCTTGCTCACTGAAGGCTCAAGTGGTAGATCAATATCATCAAAGCTGTTCTAAAATGAAAAATAAATAAATTTGTTAGTCCACTACTGGTTTCTACTCAGTGCTCATCAATGTATACATCACTAAAAACATGTGAGAAATTGAGTACTTTCTTTGTTGTATCTAAATTTTCCCTTTTAACTACTATTTATTTTTACTCAACCTTAGTAAAGGTTTTAAACAAAGGACAAAGATCTTGTATTGTTCTACAAAGGATCATATATGAACTGT
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4573
PRIMER_LEFT_PENALTY=0.385416
PRIMER_RIGHT_PENALTY=1.071885
PRIMER_LEFT_SEQUENCE=GTTGTTTCATGGAGCAAATGAG
PRIMER_RIGHT_SEQUENCE=TGATGAGCACTGAGTAGAAACCA
PRIMER_LEFT=32,22
PRIMER_RIGHT=163,23
PRIMER_LEFT_TM=59.615
PRIMER_RIGHT_TM=59.928
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=1.5336
PRIMER_LEFT_1_PENALTY=0.461740
PRIMER_RIGHT_1_PENALTY=1.071885
PRIMER_LEFT_1_SEQUENCE=CACTGAAGGCTCAAGTGGTAGA
PRIMER_RIGHT_1_SEQUENCE=TGATGAGCACTGAGTAGAAACCA
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=163,23
PRIMER_LEFT_1_TM=59.538
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=1.5336
PRIMER_LEFT_2_PENALTY=0.461740
PRIMER_RIGHT_2_PENALTY=1.071885
PRIMER_LEFT_2_SEQUENCE=TCACTGAAGGCTCAAGTGGTAG
PRIMER_RIGHT_2_SEQUENCE=TGATGAGCACTGAGTAGAAACCA
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=163,23
PRIMER_LEFT_2_TM=59.538
PRIMER_RIGHT_2_TM=59.928
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=1.5336
PRIMER_LEFT_3_PENALTY=0.461740
PRIMER_RIGHT_3_PENALTY=1.071885
PRIMER_LEFT_3_SEQUENCE=CTCACTGAAGGCTCAAGTGGTA
PRIMER_RIGHT_3_SEQUENCE=TGATGAGCACTGAGTAGAAACCA
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=163,23
PRIMER_LEFT_3_TM=59.538
PRIMER_RIGHT_3_TM=59.928
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=104
PRIMER_PAIR_PENALTY_4=1.8886
PRIMER_LEFT_4_PENALTY=0.816680
PRIMER_RIGHT_4_PENALTY=1.071885
PRIMER_LEFT_4_SEQUENCE=ATGAGGTCTTGCTCACTGAAGG
PRIMER_RIGHT_4_SEQUENCE=TGATGAGCACTGAGTAGAAACCA
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=163,23
PRIMER_LEFT_4_TM=60.817
PRIMER_RIGHT_4_TM=59.928
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_00602 Ps_contig_mira-and-tgicl-ass_franssen_5792
SEQUENCE=TTGTCGCCGGAGAACCATTTCCGGTGGATGTTTCAGAAACGACGACTGTGTACGACGGTGGTTGCGGCGGGAAGAAAGCGGACTTAGCAGCAATCGAGGACGTTACTCCGCCCATGGTTGCTTTGATTCATAAAGACGGCAGTGACGGCGGTTGCGGCGGTGAGATTTCAGATTCAGAAAGAAAGAAGAGCGGCGCGTGTAAGTTTTTTGTTTGTGGTTGGTGTAAATTAATTTCTTTAAGTTTTTTTC
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00603 Ps_contig_mira-and-tgicl-ass_franssen_5843
SEQUENCE=AATWKTAATAATAATAATAATAATGTAATAATTAACAACCAAAGTGTAGCAACCCATCAACATGTATTTATAGTTGACCCATCTAGAATTAGGAGAAACATTTTCCTAATAATAACAAATTAATGATAAGGACATAATATATTCCATTTTCGTCCTCAATCTTAACGACACGTCCTCACATTAATTCTCAATCCAAAAAATATCAATAAAAATCATTTCCCTACATTATTTATAGATGAAGACTTCCATCAAATATTTAATTAATGTAAGATATTCCATCGCGTATCAAGATTGGGGGACGAAAATGGTACTGCAAATTTACTACTAGGTAC
TARGET=6,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00604 Ps_contig_mira-and-tgicl-ass_franssen_5855
SEQUENCE=ACAATAACACCCTACAAAAAGTAACTTGATCCTAATTTGTTATCTTCTGTATAGTTCAACATCTGCACAATTAAATTTAAAACAACATGCATGAAACAAATAAATAAATAAAAAAAATTATAACTGTAACCTAGGTAAACAAAAGATTGACAAATACAACTCAACAACAGGTGGAGT
TARGET=98,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.9671
PRIMER_LEFT_PENALTY=3.592105
PRIMER_RIGHT_PENALTY=1.375005
PRIMER_LEFT_SEQUENCE=CACCCTACAAAAAGTAACTTGATCC
PRIMER_RIGHT_SEQUENCE=ACTCCACCTGTTGTTGAGTTGT
PRIMER_LEFT=7,25
PRIMER_RIGHT=176,22
PRIMER_LEFT_TM=59.408
PRIMER_RIGHT_TM=58.625
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=5.8022
PRIMER_LEFT_1_PENALTY=3.592105
PRIMER_RIGHT_1_PENALTY=2.210129
PRIMER_LEFT_1_SEQUENCE=CACCCTACAAAAAGTAACTTGATCC
PRIMER_RIGHT_1_SEQUENCE=CTCCACCTGTTGTTGAGTTGTA
PRIMER_LEFT_1=7,25
PRIMER_RIGHT_1=175,22
PRIMER_LEFT_1_TM=59.408
PRIMER_RIGHT_1_TM=57.790
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=5.9044
PRIMER_LEFT_2_PENALTY=3.592105
PRIMER_RIGHT_2_PENALTY=2.312305
PRIMER_LEFT_2_SEQUENCE=CACCCTACAAAAAGTAACTTGATCC
PRIMER_RIGHT_2_SEQUENCE=ACTCCACCTGTTGTTGAGTTGTA
PRIMER_LEFT_2=7,25
PRIMER_RIGHT_2=176,23
PRIMER_LEFT_2_TM=59.408
PRIMER_RIGHT_2_TM=58.688
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=6.0981
PRIMER_LEFT_3_PENALTY=3.592105
PRIMER_RIGHT_3_PENALTY=2.506003
PRIMER_LEFT_3_SEQUENCE=CACCCTACAAAAAGTAACTTGATCC
PRIMER_RIGHT_3_SEQUENCE=CTCCACCTGTTGTTGAGTTGTATT
PRIMER_LEFT_3=7,25
PRIMER_RIGHT_3=175,24
PRIMER_LEFT_3_TM=59.408
PRIMER_RIGHT_3_TM=59.494
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=6.2924
PRIMER_LEFT_4_PENALTY=3.592105
PRIMER_RIGHT_4_PENALTY=2.700295
PRIMER_LEFT_4_SEQUENCE=CACCCTACAAAAAGTAACTTGATCC
PRIMER_RIGHT_4_SEQUENCE=CCACCTGTTGTTGAGTTGTATTTG
PRIMER_LEFT_4=7,25
PRIMER_RIGHT_4=173,24
PRIMER_LEFT_4_TM=59.408
PRIMER_RIGHT_4_TM=60.700
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_00605 Ps_contig_mira-and-tgicl-ass_franssen_5856
SEQUENCE=ATTTTTATTCTTGTTTCTATGCATGTTGTTTAAATTTAATTGTGCAGATGTTGAACTATACAGAAGATAACAAATTAAGAATCAAGTTACTTTTCGTAGGGTGTTATTATTATTATTTTTTCTTTTGGTTCTGTAAGGGTGTTATTTTTTGTTATAATTATTTTGTAACACACCTCCTATTTTATGGGAGAAGCTATTTATTGTTGTTTAGCTATTCATCAGAAGAGCACACGTGT
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.7945
PRIMER_LEFT_PENALTY=3.473595
PRIMER_RIGHT_PENALTY=0.320870
PRIMER_LEFT_SEQUENCE=GAATCAAGTTACTTTTCGTAGGGTG
PRIMER_RIGHT_SEQUENCE=CACGTGTGCTCTTCTGATGAAT
PRIMER_LEFT=78,25
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=59.526
PRIMER_RIGHT_TM=60.321
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=4.3694
PRIMER_LEFT_1_PENALTY=3.473595
PRIMER_RIGHT_1_PENALTY=0.895776
PRIMER_LEFT_1_SEQUENCE=GAATCAAGTTACTTTTCGTAGGGTG
PRIMER_RIGHT_1_SEQUENCE=ACACGTGTGCTCTTCTGATGAA
PRIMER_LEFT_1=78,25
PRIMER_RIGHT_1=235,22
PRIMER_LEFT_1_TM=59.526
PRIMER_RIGHT_1_TM=60.896
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=4.5141
PRIMER_LEFT_2_PENALTY=3.473595
PRIMER_RIGHT_2_PENALTY=1.040498
PRIMER_LEFT_2_SEQUENCE=GAATCAAGTTACTTTTCGTAGGGTG
PRIMER_RIGHT_2_SEQUENCE=CACGTGTGCTCTTCTGATGAA
PRIMER_LEFT_2=78,25
PRIMER_RIGHT_2=234,21
PRIMER_LEFT_2_TM=59.526
PRIMER_RIGHT_2_TM=60.040
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=4.7930
PRIMER_LEFT_3_PENALTY=3.473595
PRIMER_RIGHT_3_PENALTY=1.319419
PRIMER_LEFT_3_SEQUENCE=GAATCAAGTTACTTTTCGTAGGGTG
PRIMER_RIGHT_3_SEQUENCE=CACGTGTGCTCTTCTGATGAATA
PRIMER_LEFT_3=78,25
PRIMER_RIGHT_3=234,23
PRIMER_LEFT_3_TM=59.526
PRIMER_RIGHT_3_TM=60.319
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=5.0926
PRIMER_LEFT_4_PENALTY=3.473595
PRIMER_RIGHT_4_PENALTY=1.619006
PRIMER_LEFT_4_SEQUENCE=GAATCAAGTTACTTTTCGTAGGGTG
PRIMER_RIGHT_4_SEQUENCE=TCTCCCATAAAATAGGAGGTGTG
PRIMER_LEFT_4=78,25
PRIMER_RIGHT_4=190,23
PRIMER_LEFT_4_TM=59.526
PRIMER_RIGHT_4_TM=59.381
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=113
=
PRIMER_SEQUENCE_ID=SSR_00606 Ps_contig_mira-and-tgicl-ass_franssen_5877
SEQUENCE=GATAATKAAATAAGTTTAGGAAAGAAACCGAACAAACTCCATCTTCTTCCTTGCAGTTACACTTGTGATACTCTAACCTCCAACCACTCCTACAAAAAAAATGACTCTATTTTCTTCACTGCAAATCACCACCACCGTCTCTCTTCACTCTTTCAATCCCTCCTCAACCATCTTCCACAAATCCTTTCTTCCTTGTTTCGCAACTTTACCATCTCTTTCTCTCAATAGATTCGTCTTTTCCACTCAAAAATCTCGAACAACTACTACTACTACCACCCGTGCTTCTTTTCATGAAAATCCAGTTCTATGGGTTGGTAGAATCTGCGTCTTCTATGCTCTTCTCAAGACTGGATTAGTCGGATCTCCATCAAATCCGTTCCTCTCAGATTTGGAAATTGGTAGTAATGGTGATAGTAATGATTCTGGTGACTTAGGGTTCTCTAAATGGACACAGTCCTTACTTGGAAAACCAGCCAAGGAAGAAGCTAACGGAGGGAACCTTACGAGCAAATGGCATCCTACAACAAAGGGTACACTGAGGAGGAACTACAGAGTCCCATCCAAGTCTGAGGGGCGACGACTTCTCAAAGCCATTGCATCTCTGTTGTCAGATGATGACCACTTTGTTGATGCCACTTCTCACAAGGGTTGCCAAATCCGGCGAGAAAGTGCTCATGGAGAAAGTGTTTGTTGCAACAACGTGAGAGCTCTTTTTGATGAGCTACCAACTCCTCATCTCATTGTGGAGATCACTCCTTTTCCTGTTGGACCTCTTAGTGACAAGGATTACACTAAAGCTGAGAAGCTTGAGAAGGTTCTCAGATCTAGCCCTTCTGTTTGAACCCTCCTTGATGTTTCTCGTTTTCATGTATATATTCTTTGCTAGCTTGTAAATATTGGCACCACGAAGAGAAATTTGTATAACATGTTTTACATTTGAGTTGAATCATAATCACATCGTTCAAAGACAGATAACCTGTTACCTTACTTTGTAGACGGATCTGTTCACCTTAAATCGACCAAGGAGAATGAATGTCTTGTARTAAAACT
TARGET=260,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00607 Ps_contig_mira-and-tgicl-ass_franssen_5885
SEQUENCE=AAAACAAAATTACTCAGCCAAGATACTCAAACAAACAAACTTTAAGGAAACCAAATACTTATATGAATTATAAGAAGAACAAAAGGTAGTCAGTGGTTCAGTATCTGCTATAGATTCACAGCAGCTTAATGATACCTCAAGCCTTTACCTCATTGGTATATTAGCATCAGTGAACATTGCAGTGTTTCTATTTGAAATAGCAAGTCCTGTAAGGGGTAATGACTTAGAGCTGTTTTCACTTCCATTACTATATGGAGCAAAGATAAATCATTTGATCATGGTTGGAGAATGGTGGAGGCTTATAACACCAATGTTTCTGCATGCAGGAATATTTCACTTGGGTCTAAGTTGTTGGGCCCTTGTATCATTTGGGCCTCAAGTTTGCAAAAGCTATGGTTCACTTACATTTTTCTTGATCTACATATTGGGTGGAGTTTCTGGCAACATCGCAAGTTTTCTACATACACCAAATCCAACTGTTGGTGGGACTGGGCCTGTATTTGCAATAATTGGTGCTTGGCTCATATACCAAATTCAAAATAGAGATGTTATTGCAAATGATGATTCTGAGAATCTATTCAAGAAGGCTATAATTATCACAATTCTTGGTTTCATATTATGCAACCTTGGTCCAGTTGATGAGTGGTCTCACTCAGGCGCAACTTTCACAGGCATGGTATATGGTTTTCTAACTAGTTCAACTCTTCAGCTTGATAGTACATCGCCACAAACCGGTGAAGAAGGACTCAAACTTGTTAAAAAATACGGTGGTTCTTTCAAATCACTAATTATATTCGTTATATCCATAGTTGTTTTAAGCTCATTACTTTTGTTTATGGATCCCCCTCTCAATGCACTGGCGTCTGCTGATGAAGTAGCTGTTGTAGCCTTAGAATATATGTCTAATTTTTTATTGACTGTCATGAGAGAATCTGATACTTATCAACTTAAAAGTTAGGGTTACTAATTACAGATATNTGATTGTATAAGCTCTACGTTCCGTTTCGGTAACCGTTAAAAA
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00608 Ps_contig_mira-and-tgicl-ass_franssen_5886
SEQUENCE=AGTTTTCTTGCACTTTTTCATTGGATAACGATGATGATGATGATGATGATGATGATTCCTCTACATCAACTTTCCTTTTCTAGCTCTTTTTCTAACTCCTATTTCTTCCATTGCATAGTCCTCTTCCTCCCATCATTTTTAGTTATGTTGAATTCAGCACATGTTTGTTGGTTGACAAGTTAGTATTTCTTATGATTTTCTTTTTGGGTAKTGTTTTTTATTGAGGAGGAAAGTAGAAGATCTTGGCCGTA
TARGET=30,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00609 Ps_contig_mira-and-tgicl-ass_franssen_5889
SEQUENCE=TAGAGAAGAGAAACCGCCTAAGCATCGATCTAGGTGATTGGTGGTGGTTTGTGTTGGTCGGTGGTTATAGTTGCCGGAGATGGGATATTCCGGTGAAGGTGAAAGGTTATGATGTTTTTTTGTAAWTATTAATTACTTTAGGTTTGTGGGGTAGTAGTGCCTAGTGGTTGAGTTGAGTTGAGTTCATCATGAATTTGTATTGTTAATTAAGAACATAATGATGATGGGTCATGTTTTATYTTTCCTTTTGCTACCTTTATTATTACAATAGTATTTTAGTAGTATTAAACTTTTTGTTTATGTAGTCTACAAACTCTTAATACTCTTAATTGGATTGGATAAATAG
TARGET=167,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00610 Ps_contig_mira-and-tgicl-ass_franssen_5892
SEQUENCE=GGATTGTGCCGTGGAGATTCTGACGACCGTATTTGGATTCGAGTAACCCGGTTATCGCCGGAGCATGAAACGTGGGTCCATTTACATGGATCCGGGTCGGACCAACCGAGTGAATCGGGCAGGTTGATATTTCTTTTGAGAGATAACATGACTGAAGTGTCGTCGTTTTGGGAGTGAGTGAATAGGGTAATAATGAAGAGGAAGAAAGAAAGAGTTAAAGGGTTTAGTACTATGATGTTTTTCTTTATGTTGTTGTTGTTGTGGAAGAGAAGAGAAGAGGAACGATTTTTCCTTATCTTTCTATCTTCATTTTTATGTGGAGTGAGGTAGGTGGAGAGAAATGGAGGGTTGTTGGTTCGATTTGTGGAAGATGAGAGGTGGTCGGGTGTATTTGTGTAAGGAAGACGACGGCGGAAAGAGGATGGGTCAGCGGTGTTTGTCGGCGTCGTGGAGGTGTGGGAACGGCGGAAGGCAACGGAGGGTATGATGAGTAGAGAGTGGACGTCGCGGAGATGAAGGTGAAGGGTTTGCGGCGGTGGGTAGATGGGGTTTGTGTGTCGTTGGTGGCGAAGAAGAAAAACTTCTTCTTATTTCCTGTTTTTCCTTTTTCAGCTGAGAGAGGAAAGAGAGATGAGTGGAGGTGAGATGATGAGAAGCGAAGAGGAACCATCCTCTCAACTCCCTTTTTCCTTATTTTTCTTCGTGAGTTCAGAGGTTCCAAGAATCCACCCAAAATAAATCATCCTCTCCCCTTTTGAATTGGAAAAGTCCACTTCTTAAAAATAGTATTGTATCTTATTTTGAAAACAGGTTACCAATACTTTTTTCTGTTCTGAAAATAAGGCCCCTTTTCCATTCTTGTGTCACATGGATTTCCAATTTTGTGGACTGCTTTCCTCTACCTCCCAAAAGCAAGATGTCGCACGTAATGTTTTTGTGTCGYSTMC
TARGET=202,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00611 Ps_contig_mira-and-tgicl-ass_franssen_5892
SEQUENCE=GGATTGTGCCGTGGAGATTCTGACGACCGTATTTGGATTCGAGTAACCCGGTTATCGCCGGAGCATGAAACGTGGGTCCATTTACATGGATCCGGGTCGGACCAACCGAGTGAATCGGGCAGGTTGATATTTCTTTTGAGAGATAACATGACTGAAGTGTCGTCGTTTTGGGAGTGAGTGAATAGGGTAATAATGAAGAGGAAGAAAGAAAGAGTTAAAGGGTTTAGTACTATGATGTTTTTCTTTATGTTGTTGTTGTTGTGGAAGAGAAGAGAAGAGGAACGATTTTTCCTTATCTTTCTATCTTCATTTTTATGTGGAGTGAGGTAGGTGGAGAGAAATGGAGGGTTGTTGGTTCGATTTGTGGAAGATGAGAGGTGGTCGGGTGTATTTGTGTAAGGAAGACGACGGCGGAAAGAGGATGGGTCAGCGGTGTTTGTCGGCGTCGTGGAGGTGTGGGAACGGCGGAAGGCAACGGAGGGTATGATGAGTAGAGAGTGGACGTCGCGGAGATGAAGGTGAAGGGTTTGCGGCGGTGGGTAGATGGGGTTTGTGTGTCGTTGGTGGCGAAGAAGAAAAACTTCTTCTTATTTCCTGTTTTTCCTTTTTCAGCTGAGAGAGGAAAGAGAGATGAGTGGAGGTGAGATGATGAGAAGCGAAGAGGAACCATCCTCTCAACTCCCTTTTTCCTTATTTTTCTTCGTGAGTTCAGAGGTTCCAAGAATCCACCCAAAATAAATCATCCTCTCCCCTTTTGAATTGGAAAAGTCCACTTCTTAAAAATAGTATTGTATCTTATTTTGAAAACAGGTTACCAATACTTTTTTCTGTTCTGAAAATAAGGCCCCTTTTCCATTCTTGTGTCACATGGATTTCCAATTTTGTGGACTGCTTTCCTCTACCTCCCAAAAGCAAGATGTCGCACGTAATGTTTTTGTGTCGYSTMC
TARGET=248,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00612 Ps_contig_mira-and-tgicl-ass_franssen_5892
SEQUENCE=GGATTGTGCCGTGGAGATTCTGACGACCGTATTTGGATTCGAGTAACCCGGTTATCGCCGGAGCATGAAACGTGGGTCCATTTACATGGATCCGGGTCGGACCAACCGAGTGAATCGGGCAGGTTGATATTTCTTTTGAGAGATAACATGACTGAAGTGTCGTCGTTTTGGGAGTGAGTGAATAGGGTAATAATGAAGAGGAAGAAAGAAAGAGTTAAAGGGTTTAGTACTATGATGTTTTTCTTTATGTTGTTGTTGTTGTGGAAGAGAAGAGAAGAGGAACGATTTTTCCTTATCTTTCTATCTTCATTTTTATGTGGAGTGAGGTAGGTGGAGAGAAATGGAGGGTTGTTGGTTCGATTTGTGGAAGATGAGAGGTGGTCGGGTGTATTTGTGTAAGGAAGACGACGGCGGAAAGAGGATGGGTCAGCGGTGTTTGTCGGCGTCGTGGAGGTGTGGGAACGGCGGAAGGCAACGGAGGGTATGATGAGTAGAGAGTGGACGTCGCGGAGATGAAGGTGAAGGGTTTGCGGCGGTGGGTAGATGGGGTTTGTGTGTCGTTGGTGGCGAAGAAGAAAAACTTCTTCTTATTTCCTGTTTTTCCTTTTTCAGCTGAGAGAGGAAAGAGAGATGAGTGGAGGTGAGATGATGAGAAGCGAAGAGGAACCATCCTCTCAACTCCCTTTTTCCTTATTTTTCTTCGTGAGTTCAGAGGTTCCAAGAATCCACCCAAAATAAATCATCCTCTCCCCTTTTGAATTGGAAAAGTCCACTTCTTAAAAATAGTATTGTATCTTATTTTGAAAACAGGTTACCAATACTTTTTTCTGTTCTGAAAATAAGGCCCCTTTTCCATTCTTGTGTCACATGGATTTCCAATTTTGTGGACTGCTTTCCTCTACCTCCCAAAAGCAAGATGTCGCACGTAATGTTTTTGTGTCGYSTMC
TARGET=264,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00613 Ps_contig_mira-and-tgicl-ass_franssen_5895
SEQUENCE=CAAAGCAATTTTGGTAGCTGATATACCTTCTAAGCTATTGAAGCTTTGGAGAAAGGAAGAATCCAAAATTTGCAGAAGATGGTGCTAATGATGCTGCTAATGAAGCAAGTTATTGTGAGAGTGAGTTTAATGGGAAATCACCACTTACTATACAGAACAATGCTATGCATGTATTGTTTTCTAGTTTGTTACTGCAGCTTAGTTGGTTAGACTAGTTTAACTTTTAAGTTTAGTTTTATGTATTTATTACTTAATTAAAGTTTAATTTAATTTAATTATTGTTTATTGTTCTTAATAGGTAAAATTTAATTTAGGTTAAGTTAGTTTAAATTATAGTTAAGTTGTTCTTACTTAATTACTTAAATAAAGTTACACGCTATTA
TARGET=261,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00614 Ps_contig_mira-and-tgicl-ass_franssen_5896
SEQUENCE=TTTTCCAGCGGCATCAAGACCAAGCATTTGTACAACCCGCATCTCGGTATTGCCGAAGAAGGTATCGAAAAGCTTGCGAAAARCTTGACCCATTCGATCAAAATCAAAAGAAAATCGTTGCTTGATTGAATATACRATTCTTGGAAAACCGAAAAACTAGGTTTTTTGGATTTACAAAAATGACAAAGGGAAGGGAAAGGAAAGGGGGATTGAATTGAATTGAATTGCGTTTCCTTCAACCTCCCTGTTGCGTTTGATTTAGGAAAATGAATTCCTCCCG
TARGET=209,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00615 Ps_contig_mira-and-tgicl-ass_franssen_5901
SEQUENCE=TCAACGCAGAGTACGGGGGAAATATATGAGATGTGATGGTAATTTGGCAAGGTCTCTGAGATTTTGCGACCAACCTGCTTCTTCTATCTATCGTCTTCCTCTTTTTCTTCTTCTTTTTCTTCCAAACCATTGTTAGGGTTTCAACTTTCTCTCTCTCGGAGCCTCAAATTAATCCACAATTGAACCCAATTTCGCAACCTTTTCATACTTTTCAGTTCATACAACTCACCTTATCCGAACACATCGACAACTCCGTGTGTTATCTGTCATACACCAATGTTGACCACTAGTGCAGTTGCATCTTTGCCCAAATTAACACCAGTGAGAAGAGGTGGAAACTGCATTGAAGAGAATGTTGTCCCTACATCTATATTTTCAAGAGGATTACTGAATGTTAATAACTCAAGATCATTGAGTCGCACACAGTTTCCATTTGATAGAAGAGMAGACCCGTTCTACTCAGGCAGCAACTATAATAT
TARGET=148,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00616 Ps_contig_mira-and-tgicl-ass_franssen_5913
SEQUENCE=GATCYAGAATGATAATTGTGGTTGTATCATCGTGCAGTTGAGTTCGTTCTGTCCTGTAAGCTTTAACTATTTTCTAAAATACATCATTCAATCGAATTTAAACTAATAGAACATGTTAGTCATCATCATCATCAATCTCTCTTCAACCAACGTGNTTCTTCTTCCACCACTNATCCATCGATTACGACCCCACTACAAAACCAAACCTACNTCAAGATAG
TARGET=120,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00617 Ps_contig_mira-and-tgicl-ass_franssen_5929
SEQUENCE=AATATTTCAATGATCTTTTCKAAGAAAAACGGGAGGATAGTAACACAAATTCAATGGCTCTTTCTTACACCTTCATCACTCCTTCTATACCCTCTCTTTCTCTACACAATCATCATTCATCAAAGCTTCCCCTTAGAGTTTCTTATATAAATGCTTCCTTAAACTCTTCTGATTCAAAGGCACCATCATCATCATCATCATCATCATCATCTTCTTCTTCTTCACCACAAAGTGTGCCATCTTCAACCCCTTATGTTGAGTCTAGGCCACATGAGCCTGCCTTTAACTATGCCTTTGCTAACACTAATGGCAACCCACTTTTTAGGATGCTTCAAAATACAGAATCTTCCATTGAAAGGGTGATTTTCGACTTCCGTTTCTTGGCACTCTTTGCTGTTGCAGGCTCCTTGGCTGGCTCACTCCTCTGCTTCCTAAATGGTTGTGTTTATATTGTTGGTGCATACAAAGTGTATTGGACAAGCTGTGTCAAAGGAGTTCACACTGGAAAGATGGTTCTGCTTCTGGTCGAAGCAATTGATGTTTATCTTGCTGGTACTGTTATGTTGATTTTCGGCATGGGCTTATATGGGCTGTTCATCAGCAACACGCCTCCTGATGTGCCACCTTCTGTCGACCGTGCCCTCAAAGGATCTTCTCTCTTTGGAATGTTTGCTTTGAAGGAGAGACCTAAATGGATGAAAATAAGCTCACTTGACGAATTGAAAACGAAAGTAGGGCATGTTATTGTAATGATTCTTCTTGTAAAAATGTTTGAGAGGAGCAAAATGGTTGTAATTGCAACAGGTTTAGACTTGCTTAGTTACTCAGTATGTATTTTCTTGTCATCAGCATCTTTGTATATTCTTCATAATCTACATAAGCAAGATTAGAATGTAGTAGGTAACTAGGTATTGTAATATTCTGAATTCATATATATGTTAATGGAGAATCATATGCTCTTAATCTACTTTTAAAGTTCTAGCTMTAAAATACACTTACTTAAAAA
TARGET=184,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00618 Ps_contig_mira-and-tgicl-ass_franssen_5929
SEQUENCE=AATATTTCAATGATCTTTTCKAAGAAAAACGGGAGGATAGTAACACAAATTCAATGGCTCTTTCTTACACCTTCATCACTCCTTCTATACCCTCTCTTTCTCTACACAATCATCATTCATCAAAGCTTCCCCTTAGAGTTTCTTATATAAATGCTTCCTTAAACTCTTCTGATTCAAAGGCACCATCATCATCATCATCATCATCATCATCTTCTTCTTCTTCACCACAAAGTGTGCCATCTTCAACCCCTTATGTTGAGTCTAGGCCACATGAGCCTGCCTTTAACTATGCCTTTGCTAACACTAATGGCAACCCACTTTTTAGGATGCTTCAAAATACAGAATCTTCCATTGAAAGGGTGATTTTCGACTTCCGTTTCTTGGCACTCTTTGCTGTTGCAGGCTCCTTGGCTGGCTCACTCCTCTGCTTCCTAAATGGTTGTGTTTATATTGTTGGTGCATACAAAGTGTATTGGACAAGCTGTGTCAAAGGAGTTCACACTGGAAAGATGGTTCTGCTTCTGGTCGAAGCAATTGATGTTTATCTTGCTGGTACTGTTATGTTGATTTTCGGCATGGGCTTATATGGGCTGTTCATCAGCAACACGCCTCCTGATGTGCCACCTTCTGTCGACCGTGCCCTCAAAGGATCTTCTCTCTTTGGAATGTTTGCTTTGAAGGAGAGACCTAAATGGATGAAAATAAGCTCACTTGACGAATTGAAAACGAAAGTAGGGCATGTTATTGTAATGATTCTTCTTGTAAAAATGTTTGAGAGGAGCAAAATGGTTGTAATTGCAACAGGTTTAGACTTGCTTAGTTACTCAGTATGTATTTTCTTGTCATCAGCATCTTTGTATATTCTTCATAATCTACATAAGCAAGATTAGAATGTAGTAGGTAACTAGGTATTGTAATATTCTGAATTCATATATATGTTAATGGAGAATCATATGCTCTTAATCTACTTTTAAAGTTCTAGCTMTAAAATACACTTACTTAAAAA
TARGET=211,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00619 Ps_contig_mira-and-tgicl-ass_franssen_5933
SEQUENCE=TAAAAATTGCTTAGGTTTTTTGGGTCTTTCCTCCATTCCTCAAAAGAATCAACGAACCAGGCCTCAGTTTCTTCAGTGCTTTTGCATGTAAAGTCTGGCCTGCTAGATCCACCCCAACCAAGTTGATCAATAGCAACAACTCTAAATCGCGAAGCAAGTGCATCAAAATTGCGAAAGAAGAAACCCTGTGATGCAGCATATCCATGAACCATTACTAGTGTAGGAGAATCCTCTTTACTATCAAAGGTAACAGTGTTGATAAACCGTGGCTCATTGCTAGATGAACGAAACCAGCTCACTCTGGAATCCGGTGGACCAGAACCAATATTAACCTGTTCTTGGACATAAGGAGTCTTGACAAGAGAAAGAAGGCGCTTCTCGGCATTGATGATGTGGTCGGTAGAAGTGGGGATCCAACGGCGGAGAAATGGCCAGAAACCGCCTGATTCGAGGTTGTTTTTTCCGGTGTTTTCTGCCGCCATTTTTGCTAAGAAACGGGATCGAACTGGGTTCATAGAATAACAAACTAGTAACAAATTAACTAACTAAGAAACGAACTTGTTTCTGTGATTGGAAATTGGAAATGATTATTACAACTTGGAAGAATGAATGAATGAAGAAGAAGGAGCAGTGAAATTGAGTTTTGACTGTTMCCGCGTACT
TARGET=604,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00620 Ps_contig_mira-and-tgicl-ass_franssen_5938
SEQUENCE=GGTGCATATATTAATAATAATTTAAAAATTGTTKGAGAGTTGAGAGAGTAGCGTAGTGTTGGCAATTGAAGTTCGTTTCTGGATAATAATGTTGATAACCGTAAGAGACCCTTCCCCTACTCTCCGCTGTTCTTCGCTTAACCGCCGTCTCATTCCAACAAAACACAACCTCTCTCCCCGTCTTCTCCGTTCATCTTTCCGTCAACACAACCTCTCTCTCCGTCTCCGTTCATCCTCCTCCTCCAATCTCTATACCAGTTGTGACCTAGCGAATTTCGATCCTCTAGGCATTAATCCAGATTTATCTTCATCTTCTACTTGGAGAAATCTCCTCACTATCTTTCAAACCTCTTCGCAAAAAGATAAACCTCGAGGCGTAGCAGCTGCAATTGAGGATAGTTCAATTGATTTTGGTGACTTCTTTAATGGTCCACTGCCTGGGAAGTTTCTCAAGCTTTTAGGGTTTCTTGCTTTGTCGAGGCTCGGTGTGTATATACCTCTGGGTGGAGTTAATAGGGACGCTTTTCTAGGAAATTTGGATCAGAACTCTTTGTTAACTACTTTGGATTCCTTTTCTGGTGGAGGTATTGGTAGACTTGGAATATGCTCTCTTGGCATTGTTCCGTTCATCAATGCGCAGATTGTTTTTCAGCTTCTTGCTCAAGTGTATCCCAAATTGCAAGATCTTCAGAAGAAAGAAGGTGAGGCGGGAAGAAAGAAGCTTCTTCAGTATACTCGATATGCTTCGGTTGGATTTGCTATTGTACAGGCTATCGGGCAAGTTCTTTTCCTACGGCCCTATGCAAATGATTTTACTACAGAGTGGGCGCTTACTTCTGTTATCTTACTAACACTTGGCTCTGTCTTTACTACATACATTGGGGAACAAATTACCGAACTCAAACTTGGGAATGGTACCTCTCTTTTGATATTCACAAACATTATCTCATACTTGCCGGCGTCTTTTGGTCGCACATTTTCACAGGCGTTTAGTGATGCTAACTATGTTGGACTTGTTACCATCATTGTCTCATTCTTTCTGTTGGTACTTGGTATTGTCTATGTTCAGGAAGCAGAGAGAAAAATTCCTATTAATTATGCTTCTAGATTCACCAGCAAAAGTGGAGGGATTGAAAAATCGGCTTACCTTCCGTTTAAGGTAAATAGTTCTGGGGTAATGCCTATCATTTTTTCTACATCATCATTAGCTCTTCCTGGTACTTTAGCACGCTTCACTGGTTTAAGCTCACTGAAAACTGCTGCAGTGGCTTTGAATCCAGGAGGCTCCTTCTATCTTCCATTTAACATACTTTTGATTGCCTTCTTCAACTACTATTATACTTTCCTACAACTGGATCCCGATGATGTGAGTGAACAATTGAAACGTCAAGGTGCTTCAATTCCACTTGTCAGACCAGGCAAAAGTACAGCTACATTTATTAAAACAGTTCTTAGTCGAATATCAGTTCTGGGTTCAACATTTTTGGCAATCTTGGCTGCTGGACCTGCAGTTGTTGAGCAGACCGCACACTTGACTGCATTCCGGGGGTTTGCTGGAACATCTATTCTTATTCTTGTTGGTTGTGCGACAGACACGGCCAGAAAAGTTCGAGCTGAGATTATATCCCAGAAGTACAAAAACATAGAGTTATATGATTTTGATAAGTATTGACCATTTAACAATTGGTTGTTTCCTTTGTCTAAACACACTACTAGATCATTTTATATTGACCTGTATATAATTTTGCAGAGCATTATTTTTTTATTATTTTATCTGTAATATAAAGATTAATTTGAATACATACAATTGCTTGCGWAGAACTAGTTTAATCAATCGTTGGAACTTATGG
TARGET=233,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00621 Ps_contig_mira-and-tgicl-ass_franssen_5941
SEQUENCE=GTCGGGGTAACCTTTATTTCTAGATTCTAAGKTCGGGGATGTTATCGGCATATACTAGGAATCTCATCTCGCTTCTGTCACTGCAACCGTGCATCTTAAGTTAGAACGACACAAAAAGCACACAAAAACAACATTGTTGAATCTTAAACACTATAAATCAATTCAATTCAATCAAGCATTGTTGTAAAGTTTGAGTTTTGAGTTTAAAGATCTGAGCTTTGTTGATGGATAGTTCAGAGAAGGTTGTTGCTGTGATTATGGTGGGTGGACCCACCAAAGGAACTAGATTTCGACCTCTTTCTTTCAATACTCCAAAACCCCTTTTCCCTTTGGCTGGACAACCAATGGTTCATCATCCTATTTCTGCTTGTAAAAGGATACCCAATTTAGCTCAAATATATCTCATTGGATTTCCTTACGAGGAGCGAGAATT
TARGET=158,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00622 Ps_contig_mira-and-tgicl-ass_franssen_5988
SEQUENCE=GGGAAGCACCTRRGGGTAGAGAAAAGGACGAGTTATTATCATTCCAAAGAAAAGAAGGTAGAGAAAAAAAGTGTGATTTGTGGAGCAACAATGGCAACACCAAAAAGATCATATGATCAACAACAACAACAACAAATTGTGCAACAAGTGAAGAACTCAGGAATGATTAACAACATTCAGAGTCCAAGAAGAGATGAAAGAGAAGAGGAAATGTCAAGATCAGTTCTAGCAATGTTTAGAGCAAAGGAAGAAGAAATTGAGAGGAGAAAATTGGAGGTTAGAGATAAAGTTCATGCTCATCTCGGTCGAGTACGAAAAGGAAACAAAGGAGGGTTGGCTGAGATAAGAGAAGAGATAGGTAGGGTTTCACAGACTACCAATGGAAGGAAAGTNTTGGTT
TARGET=118,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00623 Ps_contig_mira-and-tgicl-ass_franssen_5997
SEQUENCE=GAACACAAATATAAAAGTAATGTTAACGTGAGATATTTATATAGTATATAAAAATTCAACCCAGAAGAAAACAGATATTAATAATAATAAAAAAAACAATAGGAATATTTCTGGATCTGAGAAACCAGAAACTCATTCATCTTACTCACGCAAAAGGGTTCTCACTCTCGACATCTTTCAAACTCTCAATCTCTCGTTAACCCTCAATCGGCTTGCGCCAACCTCACGGTACGCCACTCCCAATCTCACTCTAAACGCGAAAACCATGGTTTCAAAAGAAGATCCAGTAAGAAGACTCACCTTCGGCGGTGATTCGGCGGCGATGCTAGGGTTTCAGATTGGCCCAAGGAGATCAAAAGGGGTCTCTGCGAAATCCCCCTTAGGGTGCTCAGAATTTTGATTGCTCTATTGATGTTTGGTCTGGAACGTGAAAACTTGGTGAAAATTAAAACTTCGGTTATTTGCAAAGCTTGGTCTTGGAAGTATCGCGTTTGAGGTTATGGCTGGGACACGGTTTTAGCTACAGGCATTTTTTTTAAGTAACACCCTCAATTGATTAAGAATGACTTTTGCATTACCGGAGAATCATATGCCGGACACTATATTCCAGCTCTCGCATCCCGGGTTCACAACGGAAACAAAAACAAAGAAGGAATTATTATAAACTTCAAGGATTTTGCCATTGGTAATGGATTGACCAACCCGGAAATTCAGTACCCAGTATGTACTTAGTTTGCAGTTGATAACAAACTGATCACAAAGGAAGATCAAGCAGATATCATCAAGTTGATCCTATTTCGTGTAGGTGCCGCAAAAAATTGTGTGAGCAAAGGTGGAGAAAGTTGCGAAACAACCTTATTACAATGTCAACCGATAGTCACTAATATCTTAGCCATTGCTGGCAATTAATTACTACGACATCAGGAAGAAATGTGAAGGAGAATTGTGTTATGACTTCTCAAGTTGGGGAAATAAGAAGACCGTTACGGATGCGATGCGTTGGGTTGGTTGGGAAAAGTAAGAGGTTTGGTAAAGTAGTTTGTAAAGTTTAATTATTACCGAATGTTATTAGAAATAGTGTGTATGCGCT
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00624 Ps_contig_mira-and-tgicl-ass_franssen_6001
SEQUENCE=TCSGGGGTACTTAAGTTCGACAGTTGTTGGATACGAAACAAACAAGCAACAAATTCATTCATTCAGATATGATTTGAGAAACCAAAACCGACGAAATTGAAGCATACCGTTGAAGAGTAAAGTGTTGTAGAATTACAAGAATCACAATCGATGGCCACTGCAACAACAACAACAACAGTAGTAAAGTATGGAATAATAGGCGTTGGAATGATGGGAAGGGAACATCTCATCAATTTGTATCAT
TARGET=53,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00625 Ps_contig_mira-and-tgicl-ass_franssen_6001
SEQUENCE=TCSGGGGTACTTAAGTTCGACAGTTGTTGGATACGAAACAAACAAGCAACAAATTCATTCATTCAGATATGATTTGAGAAACCAAAACCGACGAAATTGAAGCATACCGTTGAAGAGTAAAGTGTTGTAGAATTACAAGAATCACAATCGATGGCCACTGCAACAACAACAACAACAGTAGTAAAGTATGGAATAATAGGCGTTGGAATGATGGGAAGGGAACATCTCATCAATTTGTATCAT
TARGET=161,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00626 Ps_contig_mira-and-tgicl-ass_franssen_6025
SEQUENCE=ACTTCATGAAGGTTCGAGATTCTATCGTTAACCGGTAAGGCTTTCTTACCAGGTCCGGCCGTCCTCCCCGGTCGCTACCGGACTCACAGTTTACTTAGCTGGTGGACAAGGGCAGGTTGTTGGCGGAAGTGTTGTTGGATCTTTGATTGCTGCTGGACCTGTTATAGTTATCGCCGCTACTTTTGCTAATGCTACTTACGAGAGATTACCACTTGAGGAGGAAGAAGACGATGACGGAGGCGGGATTCACGGAGGACATCATGTTGGTGGTAATAATAATAACTCATCTCCTCCAGGAATTGGAAGCGGCGGCGGCAGTGGCGGTGGTGTGGGACATATGCAGTCTGGCCTTCCAGATCCATCTTCTTTGCCTTTGTATAACCTGCCACCAAATCTTCTCCCTAATGGTGGCCATGTGGGACATGAAGCTTTTCCATGGCCTCATGGAATTAGGCCACCATACTGAAACAAATGATATGAATCTGATGACATTAGTAAATTGGATAWTTATWTATATAGTTATTATTATATTGTTAGTTGTTTTAGTTGTAGTAATTAAAGTGGTAGTAAGTAAAAGGAAAGGTAAGGTAAGGAAAGG
TARGET=271,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00627 Ps_contig_mira-and-tgicl-ass_franssen_6041
SEQUENCE=AAGGGTGAAACGGAACGGTAAGGGTCTGTATTTTTGGGTAGGTAATGAGCAGGATCCGTTGGATCAGAAGGGTTAATAACGTTTATCTTTTGAACACGTGGGACGGYTTGAACCGTTGCTAGTCGATAAGTAAGAGTTTGTACGGATCAATTGGTAACCCTTGGATTGGGTTAGTGCTCTTTCATCTGGACCGATGATTTGGTATCTGGCACGTGCTTTTGAAATTAATTGAAACTCCTAGTTTCCTTCATTTATTTATTTTGATGAGACACCAAACAATTGTATACCCGTAAAACTAAATCTTCTATGCATATAAAATGGAAGAGAAAAAGGGTGATGAATAGTGTAAATTAATTTTACAAACT
TARGET=250,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00628 Ps_contig_mira-and-tgicl-ass_franssen_6052
SEQUENCE=CAGGTCCACCCTCGAAGGCGACTCCGAACAAGTTTCTACCGCTTTGGCTCTTTCCCCCAACGATAAACTTCTCTTCTCTTCTAGCCACAGCCGCCAAATCAGAGTTTGGGACTTATCCACTCTCAAATGCGTGCGCTCGTGGAAGGGTCACGATGGTCCTGTTATGTGTATGGCTTGTGATGCTTCTGGAGGTTTACTTGCTACTGGTGGAGCTGATAGGAAGGTCCTTGTTTGGAATGTTGATGCTGGATATTGCTCACATTTCTATAAAGGTCATGGTGGAGTTGTTTCT
TARGET=68,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2362
PRIMER_LEFT_PENALTY=0.162217
PRIMER_RIGHT_PENALTY=0.073989
PRIMER_LEFT_SEQUENCE=GAACAAGTTTCTACCGCTTTGG
PRIMER_RIGHT_SEQUENCE=TCAGCTCCACCAGTAGCAAGTA
PRIMER_LEFT=25,22
PRIMER_RIGHT=215,22
PRIMER_LEFT_TM=60.162
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.2385
PRIMER_LEFT_1_PENALTY=0.164560
PRIMER_RIGHT_1_PENALTY=0.073989
PRIMER_LEFT_1_SEQUENCE=CGACTCCGAACAAGTTTCTACC
PRIMER_RIGHT_1_SEQUENCE=TCAGCTCCACCAGTAGCAAGTA
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=215,22
PRIMER_LEFT_1_TM=60.165
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.2578
PRIMER_LEFT_2_PENALTY=0.162217
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=GAACAAGTTTCTACCGCTTTGG
PRIMER_RIGHT_2_SEQUENCE=ATCAACATTCCAAACAAGGACC
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=243,22
PRIMER_LEFT_2_TM=60.162
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.2601
PRIMER_LEFT_3_PENALTY=0.164560
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=CGACTCCGAACAAGTTTCTACC
PRIMER_RIGHT_3_SEQUENCE=ATCAACATTCCAAACAAGGACC
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=60.165
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.2601
PRIMER_LEFT_4_PENALTY=0.164560
PRIMER_RIGHT_4_PENALTY=0.095574
PRIMER_LEFT_4_SEQUENCE=GACTCCGAACAAGTTTCTACCG
PRIMER_RIGHT_4_SEQUENCE=ATCAACATTCCAAACAAGGACC
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=243,22
PRIMER_LEFT_4_TM=60.165
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.9000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_00629 Ps_contig_mira-and-tgicl-ass_franssen_6062
SEQUENCE=CCGTCCTTGCGCCCGGGCCGTAAATACCCCTTTTCGGTAGATGCTTTCAGTGGTCCTGAGATGTGGGCTAAGCTGACGGCTGATCCTACAACTAGGGCTTTTCTTCAACAACCTGATTTTGTCAAGATGATGCAAGATATTCAGAAAAATCCGAATAATCTTAATTTGTATCTGAAGGATCAAAGAGTTATGCAAGCTTTTGGGGTTTTGCTTAATGTTAAGCTTGGAAGTCCTGGTGATGATGTGGACATGCCTGATACTCCTTCTTCCTCTTCTTCTGAGAGGAAGAGAGCGGCTGAAGCTGAGCCTCCTAAGCAGCCTGAGCCGGAGCCTGAACCTGAACCCATGGAAGAGGCTGATGAGGAAAAGGAAGCTAAGCAGAGAAAGGCTGAGGCACAGAAGGAAAAGGAGGCTGGTAATGCAGCGTACAAAAAGAAGGATTTTGATACTGCAATTCACCATTACTCAAAAGCTTTGGAATTGGATGATGAAGATATATCGTTTCTCACCAACCGTGCTGCTGTATACTTGGAGATGGGAAAGTATGAGGATTGCATTAAAGATTGTGACAAGGCAGTTGAAAGAGGACGGGAACTACGATCAGACTACAAGATGGTAGCAAGAGCTTTGACAAGGAAGGGAACTGCCATGGGCAAAACGGCAAAATGCTCTAAAGATTATGAACCTGTCATTGAGACATTTCAAAAAGCACTTACCGAGCACCGCAACCCAGACACCCTAAAGAAACTGAATGAAGCTGAAAAGGCCAAGAAAGAATTAGAACAACAAGAATACTTTGATCCAAATTTGGCTGACGAGGAGCGAGAAAAAGGGAATGAATTCTTTAAGCAGCAGAAGTATCCCGAAGCTATTAGGCATTACACCGAGTCTCTAAAAAGAAACCCGAAAGATCCTAGGGCTTATAGTAACAGAGCTGCATGTTACACCAAATTGGGGGCAATGCCTGAAGGTTTAAAGGATGCAGAGAAGTGCATTGAGCTTGATCCAACCTTCACCAAGGGTTATACCAGGAAAGGTGCAGTTCAGTTTTTCATGAAAGAATATGAAAAGGCTTTGGAAACATATAAGGAGGGGTTGAAACATGATGCCAACAACCAGGAGTTGCTTGATGGCATTGGAAGGTGTGTACAACAAATTAACAGAGCCAGCCGTGGAGATTTAAGTCCTGAAGAGTTGAAGGATAGACAGGCCAAGGCAATGTCAGATCCAGAGATACAGAATATCCTTCAAGACCCTGTTATGCGACAGGTATTGGTTGACTTCCAGGAAAATCCCAAGGCTGCACAGGAACATACCAAGAACCCGGGTGTGATGGACAAGATCCAAAAGCTGATCAGTGCTGGAATTGTCCAGATGAGGTAAACTTTGTGGAAGATGGCAGCTCTCGCATAAAAGCAATTCCTACAGTGACTTTAATTTGATATTTGATAGATTGGATTGGATGTAAAATGGGAAATTTTGACTTTGTTTTTCAGTTTGTTTTCGTGATTTTTTATATTTTATAACAAACTCGAGTGCAATTCATTTTAGCATAGTAGTAGTAGTTGGTTTCGTATGTATTGACTTGGCTTTTTATAATTTAATCTTAATCCGTAWCTAAACGT
TARGET=1554,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00630 Ps_contig_mira-and-tgicl-ass_franssen_6088
SEQUENCE=GGCTATGGTTGGGGACTTGAGAAAAGAAAGAAAAAATTGAGATAGAAGAGAAAGATGAAAACATTATCATCATCTTCATCACATTCTTTCAATTTCATTCACCCTTCCAACTTCAACTATTCTTCTTCTTCATCTTCTGCATTTTCTCCTTCTTCT
TARGET=120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.8348
PRIMER_LEFT_PENALTY=0.213575
PRIMER_RIGHT_PENALTY=2.621188
PRIMER_LEFT_SEQUENCE=TGGGGACTTGAGAAAAGAAAGA
PRIMER_RIGHT_SEQUENCE=GAAGAAGGAGAAAATGCAGAAG
PRIMER_LEFT=9,22
PRIMER_RIGHT=154,22
PRIMER_LEFT_TM=60.214
PRIMER_RIGHT_TM=57.379
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=2.8856
PRIMER_LEFT_1_PENALTY=0.264444
PRIMER_RIGHT_1_PENALTY=2.621188
PRIMER_LEFT_1_SEQUENCE=TTGGGGACTTGAGAAAAGAAAG
PRIMER_RIGHT_1_SEQUENCE=GAAGAAGGAGAAAATGCAGAAG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=154,22
PRIMER_LEFT_1_TM=59.736
PRIMER_RIGHT_1_TM=57.379
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=2.9657
PRIMER_LEFT_2_PENALTY=0.344527
PRIMER_RIGHT_2_PENALTY=2.621188
PRIMER_LEFT_2_SEQUENCE=CTATGGTTGGGGACTTGAGAAA
PRIMER_RIGHT_2_SEQUENCE=GAAGAAGGAGAAAATGCAGAAG
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=57.379
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=153
PRIMER_PAIR_PENALTY_3=3.0114
PRIMER_LEFT_3_PENALTY=0.390220
PRIMER_RIGHT_3_PENALTY=2.621188
PRIMER_LEFT_3_SEQUENCE=GTTGGGGACTTGAGAAAAGAAA
PRIMER_RIGHT_3_SEQUENCE=GAAGAAGGAGAAAATGCAGAAG
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=154,22
PRIMER_LEFT_3_TM=59.610
PRIMER_RIGHT_3_TM=57.379
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=3.3144
PRIMER_LEFT_4_PENALTY=0.693167
PRIMER_RIGHT_4_PENALTY=2.621188
PRIMER_LEFT_4_SEQUENCE=TATGGTTGGGGACTTGAGAAAA
PRIMER_RIGHT_4_SEQUENCE=GAAGAAGGAGAAAATGCAGAAG
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=154,22
PRIMER_LEFT_4_TM=60.693
PRIMER_RIGHT_4_TM=57.379
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_00631 Ps_contig_mira-and-tgicl-ass_franssen_6092
SEQUENCE=CCTTTCGTCTCCGTTTCCTATCGCAAAACCCAACCTTTACCTTCTCCAATCACCACTCTCAACCCCTCTCCATTTCTGCCTCACCTCCAAAACCATGCAACCGGCTCGGTTTCGTTTTTTCTCAATGGCTGCTGCTGAGCCGAAGGAATCGCCGGCTAACAACCCCGGTCTCCATGCAACTATAGATGAAGCTACTAAGGGCTACTTTATGCAACAAACTATGTATAGAATCAAAGACCCCAAAGTTAGCCTTGATTTTTACTCCCGCATTTTGGGCATGTCTTTGCTCAAGAGACTGGATTTTCCCGAGTTGAAGTTCAGCTTGTACTTTATGGGGTATGAGGATACATCAGAAGCTCCAACTAACCCTGTTGATAGAACAGTTTGGACCTTTGCTCAAAAGGCTACAATTGAATTGACACATAATTGGGGCACCGAAAGTGATCCAGAGTTCAAAGGGCACCACAATGGCAATTCCGATCCTCGTGGTTTTGGACATATTGGCATAACTGTTGATGACACCTACAAAGCATGTGAAAGATTTCAGAATCTGGGGGTTGAGTTTGTTAAGAAACCAGATGATGGAAAAATGAAAGGGATAGCATTTATTAAGGATCCCGATGGATACTGGATTGAAATCTTTGACCGGAAAACAATAGGAAATGTAACAGAAGCTGCTGCTGCTTAAGATAAAATCTTCAGGTAGAAGCCGTGGTTTCTAATTCCGTTGTCTTATTTACTTATTCACGTTTTTCGTCCTAAAAGTAATGATGTCCCTACTTCATATTCTCTGATGATATAAAGAACCACGTTACCTGCATGTGAAAATTTGTTTCATTGTTCTGAATTATGTATAACCATGTGAAATCGCTTTCTCCAAATTAGTTTGCTATAATGCATATTTGTAGCTCAGAAACAAATCACGTGATTTTG
TARGET=674,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3992
PRIMER_LEFT_PENALTY=0.182645
PRIMER_RIGHT_PENALTY=0.216588
PRIMER_LEFT_SEQUENCE=AAGATTTCAGAATCTGGGGGTT
PRIMER_RIGHT_SEQUENCE=CACGGCTTCTACCTGAAGATTT
PRIMER_LEFT=537,22
PRIMER_RIGHT=713,22
PRIMER_LEFT_TM=60.183
PRIMER_RIGHT_TM=59.783
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.4755
PRIMER_LEFT_1_PENALTY=0.258908
PRIMER_RIGHT_1_PENALTY=0.216588
PRIMER_LEFT_1_SEQUENCE=AATCTGGGGGTTGAGTTTGTTA
PRIMER_RIGHT_1_SEQUENCE=CACGGCTTCTACCTGAAGATTT
PRIMER_LEFT_1=547,22
PRIMER_RIGHT_1=713,22
PRIMER_LEFT_1_TM=59.741
PRIMER_RIGHT_1_TM=59.783
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.5351
PRIMER_LEFT_2_PENALTY=0.283783
PRIMER_RIGHT_2_PENALTY=0.251350
PRIMER_LEFT_2_SEQUENCE=GACCGGAAAACAATAGGAAATG
PRIMER_RIGHT_2_SEQUENCE=GCAAACTAATTTGGAGAAAGCG
PRIMER_LEFT_2=643,22
PRIMER_RIGHT_2=889,22
PRIMER_LEFT_2_TM=59.716
PRIMER_RIGHT_2_TM=60.251
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.5394
PRIMER_LEFT_3_PENALTY=0.322824
PRIMER_RIGHT_3_PENALTY=0.216588
PRIMER_LEFT_3_SEQUENCE=GCATTTATTAAGGATCCCGATG
PRIMER_RIGHT_3_SEQUENCE=CACGGCTTCTACCTGAAGATTT
PRIMER_LEFT_3=601,22
PRIMER_RIGHT_3=713,22
PRIMER_LEFT_3_TM=59.677
PRIMER_RIGHT_3_TM=59.783
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=0.5742
PRIMER_LEFT_4_PENALTY=0.322824
PRIMER_RIGHT_4_PENALTY=0.251350
PRIMER_LEFT_4_SEQUENCE=GCATTTATTAAGGATCCCGATG
PRIMER_RIGHT_4_SEQUENCE=GCAAACTAATTTGGAGAAAGCG
PRIMER_LEFT_4=601,22
PRIMER_RIGHT_4=889,22
PRIMER_LEFT_4_TM=59.677
PRIMER_RIGHT_4_TM=60.251
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.6000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_00632 Ps_contig_mira-and-tgicl-ass_franssen_6106
SEQUENCE=GGTTAACAARABTTTATAATAACACTTCTTTTTTTCCAATTTGGATCTCATGCAAGCTAATAAACATGTATAACTTCGCTACAATGAATTATTACATACAGCCAAAGAAGAAAATAAGTTTATGCAAAGATTATGCCTAAAATGATGGATTTCAATATTCTTATGGACTGGTGAAAATGTGTTGAGGTACTACAACTCTGCTGTGAATTTTGTAGAATGAAACTGTCATTCTGAACCAAACCAAACAATCAATTTCAATTCTTATTGCATTAACTTGTGAATTCATATTCGTTGGCGGTATCGGATGCATTCACTCTAACTCAGCTGGAGTTTGCGCAATGACAGAGGTTGATAGTGAGGGTCGTAGGAGAATGTATATAGCAGGACCCAGCAATGGTATCAATGATACTGGAAGAAGCCAAGAACCTTTGTCAAACCATTTTCTAGCTGTCATGTCATTGTAAACCCAAAATGGTGCCAATGTTGAGAGTAATGTAAAATCAATGGACATAATATGGATAAATTTGCTTCCTCTGCTATACTGGTAAAATTCTTTCCATACATCTCCCCCGGCTAAACCGGCGTAAATTATGAGGCCTATCCCCGAAGCAAGCAGTATCGATGCAGTAACTTTTGATTCCAGAAAATTGAGTGGCCATGTTTTAAGCTGAGCTTCTTCGACAGGAGGTGGTGGAGGTTTCCATAGCACAAAATAAGGAAGAAGTGCATATATGCCGCCGAAAAACGAGAGTGACAGGAAAGGCCATACAGGAACCTTGCTTTTTGAGCTTCTTCCGGTGGGAAGTAACAGCATGCTATATACCAAAGGCCACAAGCCCATTAGATACCACTCAGATACAATCACTTCATTCATTCTGAAACCATCACCTCCTTTCAAATTCAACAGCTTTTCCAAGAAATACATGTCCATAGACGGTGTTTGGTTTGGAGAGAGAAAGGAGACATAGTAGATAAGTGCAGCCCATAAGAGAAATAGCAAAATTGAGGTTGTCCAATCTCTTTTTCCTTCCTCCACAACCTCAACAACGCTTCTGCTAACCTCACTCTCTTTGTTGGAATCATGGCAAATAACAATACTACCCTTTTTCCGAAACGAAGCAGCAAAGGGTTTTTTTGGTCATCACCTTGCAGGAGCTACTGAGTCTGATGAAACAACATCGTGAGACCAATGCTGTTGTTGTTGTAGTTGATGTATGTGGTTGAATTGAATACTTGTTTTTGAGAGTTGGAAAGTTGCAAGAGACAAGATTGGGTTTAGCTACTACTCCCACCATAATGTTTTCTCTCATAAGATACACAAACAACATCMATTHCAATGTGATATAGTC
TARGET=1194,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00633 Ps_contig_mira-and-tgicl-ass_franssen_6143
SEQUENCE=GATCACTCTCATACCTAATTCATTWACCGAAAAAAWCCTATTCGTATCCGATTACTCACAAACTCTAAGCTAAGCAAAGCAATGACGCAAGAAGAGAACAATCAACCAACCTTCGAGACGGAGGTGTGTCCCGGGTTTGTTGCAATGGACAAGTCACGGACGGAACCGAACTTGCACAACTTCTGAGAGGTGTTACTTAAACTGTATGAAACTATGCGAAACCGGATCAACTGTAAAACTGTTTGGACTGAGCTACTTGTGCATTGGCTTTGGTTACTTGAAGGAACTGTTGGTATAGGAAAGTTCTAAACCCTGCTACGCCAATTGTCGATGGATGGATGCACGGTATGCCGTAGGTTTGTTAGCTTTCAAATTCTGGTGGAACTATTAATGTAGTTTGGTAGGATTTTGGAGTTAATGGTTGGATTTTAATTATTTGTTAGTG
TARGET=330,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00634 Ps_contig_mira-and-tgicl-ass_franssen_6161
SEQUENCE=AGTTGTGATTTAGCCAAAGGAAAAGCAGAAGATGATGTTTTGTGATTCTGGAAATCATTGTGGATGGTGATGTGAGGCGCGGGAAGAAGAAGAAGATATAGGTGTTGTTTGTTAGCAGAAAAGTGGGGAGAGTGAAAGCTCCGATCTTAGCTCATGAGTTTATATCGAGTTGTTTGCAATCGGATGTGAGAGCTTGAAGTTTGAACTTGGTCGCGTTTTCAATTCATGTTGCATTTCATTTTTGTCATGCTGCATTTTGGCTTGCATTTTGGTTTGTGCTGCAATTTCTTGGCAATGTTGCAATTGCGTTTCGTTTCACTTCATGCTGCATTTCATTTTGTAAATTACTGCGTTTTGGCTTTGCAATGCCTACATTTCCAATTGCCTTTTTTTCCTTTTTTGTCTTATTTGATATAGTACTTGGACCTTGCTTTTGTTGTCTTATTTGGTATGCTTCGATATGGTTACTATAATTTGAATCTTTGATATTGGATATATATAATGGATTTTGTTGGATATAC
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0521
PRIMER_LEFT_PENALTY=0.017127
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=TTAGCCAAAGGAAAAGCAGAAG
PRIMER_RIGHT_SEQUENCE=CCCCACTTTTCTGCTAACAAAC
PRIMER_LEFT=9,22
PRIMER_RIGHT=127,22
PRIMER_LEFT_TM=60.017
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.3004
PRIMER_LEFT_1_PENALTY=0.058624
PRIMER_RIGHT_1_PENALTY=0.241736
PRIMER_LEFT_1_SEQUENCE=GAAATCATTGTGGATGGTGATG
PRIMER_RIGHT_1_SEQUENCE=GAAATGCAGCATGAAGTGAAAC
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=333,22
PRIMER_LEFT_1_TM=60.059
PRIMER_RIGHT_1_TM=59.758
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=0.3753
PRIMER_LEFT_2_PENALTY=0.017127
PRIMER_RIGHT_2_PENALTY=0.358210
PRIMER_LEFT_2_SEQUENCE=TTAGCCAAAGGAAAAGCAGAAG
PRIMER_RIGHT_2_SEQUENCE=GCTAAGATCGGAGCTTTCACTC
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=150,22
PRIMER_LEFT_2_TM=60.017
PRIMER_RIGHT_2_TM=59.642
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.3753
PRIMER_LEFT_3_PENALTY=0.017127
PRIMER_RIGHT_3_PENALTY=0.358210
PRIMER_LEFT_3_SEQUENCE=TTAGCCAAAGGAAAAGCAGAAG
PRIMER_RIGHT_3_SEQUENCE=GAGCTAAGATCGGAGCTTTCAC
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=152,22
PRIMER_LEFT_3_TM=60.017
PRIMER_RIGHT_3_TM=59.642
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=0.4168
PRIMER_LEFT_4_PENALTY=0.058624
PRIMER_RIGHT_4_PENALTY=0.358210
PRIMER_LEFT_4_SEQUENCE=GAAATCATTGTGGATGGTGATG
PRIMER_RIGHT_4_SEQUENCE=GCTAAGATCGGAGCTTTCACTC
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=150,22
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=59.642
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_00635 Ps_contig_mira-and-tgicl-ass_franssen_6172
SEQUENCE=AAATTAAATTAATGACAGTAACAAAACATCATGTAAAATAAATGGTAAGTAAGTATATTTGGAGTTTGAAATAGGGAAATGGTAGTTACCTGWYTTTAGGTTGTGCCAGATGTTGTTGGCGGTGGCTTCTGCTTTTTTGCTCCAAGAATCGAACATGTGGAGAATTGATTCCATGGGACTGTTGGCAGGTTTTTCGACAGGGGTGTGTTGAATATAAGGATGATGATCATGTTGGTAATACTGAACCTGAGGTTGTTGTTGTTGTTGTGGATCGGTTTGTAAAGCTGCTTTTTTGTTGTCTGGGTGACTACTTGGGACTGCTGGTGCTCCCATCATGTGAGTTCCCCAATTCTCAGATGTAGAAGGAGGTGATGACGGTGATGCAAATGGGTTTTCATCTTGGGATTGGGTTTTGTGATTGTTGTTGTTGTTCATGTTTTTGTATGGGTGAGAAAGAAAAAAAGGTTAGTGATGAGAGATTGAGAAAAAGGATSAAAAAGAAAATAGAAAATGGTTAGATTTAAGGAGAGAAGAA
TARGET=252,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00636 Ps_contig_mira-and-tgicl-ass_franssen_6172
SEQUENCE=AAATTAAATTAATGACAGTAACAAAACATCATGTAAAATAAATGGTAAGTAAGTATATTTGGAGTTTGAAATAGGGAAATGGTAGTTACCTGWYTTTAGGTTGTGCCAGATGTTGTTGGCGGTGGCTTCTGCTTTTTTGCTCCAAGAATCGAACATGTGGAGAATTGATTCCATGGGACTGTTGGCAGGTTTTTCGACAGGGGTGTGTTGAATATAAGGATGATGATCATGTTGGTAATACTGAACCTGAGGTTGTTGTTGTTGTTGTGGATCGGTTTGTAAAGCTGCTTTTTTGTTGTCTGGGTGACTACTTGGGACTGCTGGTGCTCCCATCATGTGAGTTCCCCAATTCTCAGATGTAGAAGGAGGTGATGACGGTGATGCAAATGGGTTTTCATCTTGGGATTGGGTTTTGTGATTGTTGTTGTTGTTCATGTTTTTGTATGGGTGAGAAAGAAAAAAAGGTTAGTGATGAGAGATTGAGAAAAAGGATSAAAAAGAAAATAGAAAATGGTTAGATTTAAGGAGAGAAGAA
TARGET=419,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00637 Ps_contig_mira-and-tgicl-ass_franssen_6181
SEQUENCE=CAAATATGAACAATATAAGAAACCCAAAACAAGGCTTAAGGTAACACATAAACTAACAAAAGACATGGTCTCTTTTCTATCAAAATTTCACATCTTCATTCTATCAAATATACAAAAATAATAATTAAATATCAACTATGAAAAATTTCGGAGTAATTAAGGAGGTAAATGAAGTGATATCCGACAACAAATCTAAGTACCTAGGAGTAAGCTAAAATCAAAACCACAAAAGGATGGACCATGCAACTTCTTCAAATGAAGGACAAAGGTGAATGAATGAATTTCTTCTATATCTTGAAAACTTTAGTTAAGATATTGGTACTATTTTTCTAAGACCTATCTTAACTAATTGTGNTTTTTCTTTCTAAGGGAAAAGTCTTACCTTACAAGTAGAGGGCGATGACTTATGNGTTGTGTGAATAGANAATTTA
TARGET=270,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7670
PRIMER_LEFT_PENALTY=0.360320
PRIMER_RIGHT_PENALTY=0.406645
PRIMER_LEFT_SEQUENCE=GGACCATGCAACTTCTTCAAAT
PRIMER_RIGHT_SEQUENCE=CNCATAAGTCATCGCCCTCTAC
PRIMER_LEFT=235,22
PRIMER_RIGHT=410,22
PRIMER_LEFT_TM=60.360
PRIMER_RIGHT_TM=60.407
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.7670
PRIMER_LEFT_1_PENALTY=0.360320
PRIMER_RIGHT_1_PENALTY=0.406645
PRIMER_LEFT_1_SEQUENCE=GGACCATGCAACTTCTTCAAAT
PRIMER_RIGHT_1_SEQUENCE=ACNCATAAGTCATCGCCCTCTA
PRIMER_LEFT_1=235,22
PRIMER_RIGHT_1=411,22
PRIMER_LEFT_1_TM=60.360
PRIMER_RIGHT_1_TM=60.407
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.50
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.7921
PRIMER_LEFT_2_PENALTY=0.385416
PRIMER_RIGHT_2_PENALTY=0.406645
PRIMER_LEFT_2_SEQUENCE=GACCATGCAACTTCTTCAAATG
PRIMER_RIGHT_2_SEQUENCE=CNCATAAGTCATCGCCCTCTAC
PRIMER_LEFT_2=236,22
PRIMER_RIGHT_2=410,22
PRIMER_LEFT_2_TM=59.615
PRIMER_RIGHT_2_TM=60.407
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=175
PRIMER_PAIR_PENALTY_3=0.7921
PRIMER_LEFT_3_PENALTY=0.385416
PRIMER_RIGHT_3_PENALTY=0.406645
PRIMER_LEFT_3_SEQUENCE=GACCATGCAACTTCTTCAAATG
PRIMER_RIGHT_3_SEQUENCE=ACNCATAAGTCATCGCCCTCTA
PRIMER_LEFT_3=236,22
PRIMER_RIGHT_3=411,22
PRIMER_LEFT_3_TM=59.615
PRIMER_RIGHT_3_TM=60.407
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.50
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=1.2429
PRIMER_LEFT_4_PENALTY=0.360320
PRIMER_RIGHT_4_PENALTY=0.882618
PRIMER_LEFT_4_SEQUENCE=GGACCATGCAACTTCTTCAAAT
PRIMER_RIGHT_4_SEQUENCE=CACACAACNCATAAGTCATCGC
PRIMER_LEFT_4=235,22
PRIMER_RIGHT_4=417,22
PRIMER_LEFT_4_TM=60.360
PRIMER_RIGHT_4_TM=60.883
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.50
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_00638 Ps_contig_mira-and-tgicl-ass_franssen_6202
SEQUENCE=TTGGGGGAGGGTGAAGAGAAGAAAAGAAAAGGCTGCGAATTTAAAAACGTTTTCTCTGTTTGAGTATATCCACTGAAAAGAGCGCAGCCAGGAGAGAGAACAAAACCCTTTCAAAGGTTTTGAAGATAACAACAATGAATACTTGTTCTTAGACCCAACGGAACAACGTTGTTGTAGTAACAAGTAACCACAAATGCTTCCGCTTAGAAGAAGAAGAACAAGTAGTAACGAATAATAATAATAATGCAGAAACTTCTTCAGCGTCCAACTTGTTCACTCCCAATCCCTATTCTATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCCAACCCTCTCTTTCTCCATCTCCCAACAACAATGTCTACAGCAGCAGCAACAACAACGCCGTCACTCCATTAAGTAATTTCATTCCC
TARGET=206,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0705
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_SEQUENCE=GCTGTAGACATTGTTGTTGGGA
PRIMER_LEFT=140,22
PRIMER_RIGHT=370,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.1078
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.074695
PRIMER_LEFT_1_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_1_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_LEFT_1=140,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.075
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.1692
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_2_SEQUENCE=TAGGGATTGGGAGTGAACAAGT
PRIMER_LEFT_2=140,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.2001
PRIMER_LEFT_3_PENALTY=0.125411
PRIMER_RIGHT_3_PENALTY=0.074695
PRIMER_LEFT_3_SEQUENCE=TATATCCACTGAAAAGAGCGCA
PRIMER_RIGHT_3_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=351,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.2201
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_4_SEQUENCE=GAATAGGGATTGGGAGTGAACA
PRIMER_LEFT_4=140,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_00639 Ps_contig_mira-and-tgicl-ass_franssen_6202
SEQUENCE=TTGGGGGAGGGTGAAGAGAAGAAAAGAAAAGGCTGCGAATTTAAAAACGTTTTCTCTGTTTGAGTATATCCACTGAAAAGAGCGCAGCCAGGAGAGAGAACAAAACCCTTTCAAAGGTTTTGAAGATAACAACAATGAATACTTGTTCTTAGACCCAACGGAACAACGTTGTTGTAGTAACAAGTAACCACAAATGCTTCCGCTTAGAAGAAGAAGAACAAGTAGTAACGAATAATAATAATAATGCAGAAACTTCTTCAGCGTCCAACTTGTTCACTCCCAATCCCTATTCTATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCCAACCCTCTCTTTCTCCATCTCCCAACAACAATGTCTACAGCAGCAGCAACAACAACGCCGTCACTCCATTAAGTAATTTCATTCCC
TARGET=231,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0705
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_SEQUENCE=GCTGTAGACATTGTTGTTGGGA
PRIMER_LEFT=140,22
PRIMER_RIGHT=370,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.1078
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.074695
PRIMER_LEFT_1_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_1_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_LEFT_1=140,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.075
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.1692
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_2_SEQUENCE=TAGGGATTGGGAGTGAACAAGT
PRIMER_LEFT_2=140,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.2001
PRIMER_LEFT_3_PENALTY=0.125411
PRIMER_RIGHT_3_PENALTY=0.074695
PRIMER_LEFT_3_SEQUENCE=TATATCCACTGAAAAGAGCGCA
PRIMER_RIGHT_3_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=351,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.2201
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_4_SEQUENCE=GAATAGGGATTGGGAGTGAACA
PRIMER_LEFT_4=140,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_00640 Ps_contig_mira-and-tgicl-ass_franssen_6202
SEQUENCE=TTGGGGGAGGGTGAAGAGAAGAAAAGAAAAGGCTGCGAATTTAAAAACGTTTTCTCTGTTTGAGTATATCCACTGAAAAGAGCGCAGCCAGGAGAGAGAACAAAACCCTTTCAAAGGTTTTGAAGATAACAACAATGAATACTTGTTCTTAGACCCAACGGAACAACGTTGTTGTAGTAACAAGTAACCACAAATGCTTCCGCTTAGAAGAAGAAGAACAAGTAGTAACGAATAATAATAATAATGCAGAAACTTCTTCAGCGTCCAACTTGTTCACTCCCAATCCCTATTCTATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCCAACCCTCTCTTTCTCCATCTCCCAACAACAATGTCTACAGCAGCAGCAACAACAACGCCGTCACTCCATTAAGTAATTTCATTCCC
TARGET=295,30
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0705
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_SEQUENCE=GCTGTAGACATTGTTGTTGGGA
PRIMER_LEFT=140,22
PRIMER_RIGHT=370,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.1078
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.074695
PRIMER_LEFT_1_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_RIGHT_1_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_LEFT_1=140,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.075
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.1090
PRIMER_LEFT_2_PENALTY=0.071684
PRIMER_RIGHT_2_PENALTY=0.037339
PRIMER_LEFT_2_SEQUENCE=GAAACTTCTTCAGCGTCCAACT
PRIMER_RIGHT_2_SEQUENCE=GCTGTAGACATTGTTGTTGGGA
PRIMER_LEFT_2=248,22
PRIMER_RIGHT_2=370,22
PRIMER_LEFT_2_TM=59.928
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.1464
PRIMER_LEFT_3_PENALTY=0.071684
PRIMER_RIGHT_3_PENALTY=0.074695
PRIMER_LEFT_3_SEQUENCE=AGAAACTTCTTCAGCGTCCAAC
PRIMER_RIGHT_3_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_LEFT_3=247,22
PRIMER_RIGHT_3=351,22
PRIMER_LEFT_3_TM=59.928
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.2001
PRIMER_LEFT_4_PENALTY=0.125411
PRIMER_RIGHT_4_PENALTY=0.074695
PRIMER_LEFT_4_SEQUENCE=TATATCCACTGAAAAGAGCGCA
PRIMER_RIGHT_4_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_LEFT_4=64,22
PRIMER_RIGHT_4=351,22
PRIMER_LEFT_4_TM=59.875
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_00641 Ps_contig_mira-and-tgicl-ass_franssen_6237
SEQUENCE=GTGGTATCAACGCAGAGTTGAAACGCATACCAAACTTCCAAATGAACCAAGCGTAAGCGGCTACAATACAAGCCAAGCTACTTCTAAGCTCCAAGTTCTGCAATAAAAAACGCACGTGACCAAAAACCATAACCAATCCATAACAGAAAATTCATAACTAACTAACAAACTCTAACAGAGAAATCTGACAAAAACNGGGCTACTAAGTAAAACGGGGAACCCCACGCATCAATCTAGGTTTCTTCTTCAATCTCCACTTC
TARGET=155,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2561
PRIMER_LEFT_PENALTY=0.165541
PRIMER_RIGHT_PENALTY=0.090580
PRIMER_LEFT_SEQUENCE=CAGAGTTGAAACGCATACCAAA
PRIMER_RIGHT_SEQUENCE=CCCNGTTTTTGTCAGATTTCTC
PRIMER_LEFT=12,22
PRIMER_RIGHT=198,22
PRIMER_LEFT_TM=60.166
PRIMER_RIGHT_TM=59.909
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.2598
PRIMER_LEFT_1_PENALTY=0.169174
PRIMER_RIGHT_1_PENALTY=0.090580
PRIMER_LEFT_1_SEQUENCE=GCCAAGCTACTTCTAAGCTCCA
PRIMER_RIGHT_1_SEQUENCE=CCCNGTTTTTGTCAGATTTCTC
PRIMER_LEFT_1=71,22
PRIMER_RIGHT_1=198,22
PRIMER_LEFT_1_TM=60.169
PRIMER_RIGHT_1_TM=59.909
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=128
PRIMER_PAIR_PENALTY_2=0.6681
PRIMER_LEFT_2_PENALTY=0.165541
PRIMER_RIGHT_2_PENALTY=0.502561
PRIMER_LEFT_2_SEQUENCE=CAGAGTTGAAACGCATACCAAA
PRIMER_RIGHT_2_SEQUENCE=AGAAACCTAGATTGATGCGTGG
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=242,22
PRIMER_LEFT_2_TM=60.166
PRIMER_RIGHT_2_TM=60.503
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.6717
PRIMER_LEFT_3_PENALTY=0.169174
PRIMER_RIGHT_3_PENALTY=0.502561
PRIMER_LEFT_3_SEQUENCE=GCCAAGCTACTTCTAAGCTCCA
PRIMER_RIGHT_3_SEQUENCE=AGAAACCTAGATTGATGCGTGG
PRIMER_LEFT_3=71,22
PRIMER_RIGHT_3=242,22
PRIMER_LEFT_3_TM=60.169
PRIMER_RIGHT_3_TM=60.503
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.6978
PRIMER_LEFT_4_PENALTY=0.165541
PRIMER_RIGHT_4_PENALTY=0.532249
PRIMER_LEFT_4_SEQUENCE=CAGAGTTGAAACGCATACCAAA
PRIMER_RIGHT_4_SEQUENCE=GTAGCCCNGTTTTTGTCAGATT
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=202,22
PRIMER_LEFT_4_TM=60.166
PRIMER_RIGHT_4_TM=59.468
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_00642 Ps_contig_mira-and-tgicl-ass_franssen_6242
SEQUENCE=TCCAAGATCTATAGGAAGTAGTGAGTTTCTCAACCCAATAACTCATGAAGTCATTTTGAGTAACTCAAGGTGTGAGTTAAAAATCACTGGTTTTCAAGAAGGGGTTAATAGTGGTGGTAACAATGCTAGTGGAAGTAGTGGTGGTGGTTCTTCAACTTTTGTTGGTACTTTGAGACCTGGAACACCTGGACCTGGAGGACACCCTACAATGCATTACTTTAACCCTTCTTTTAGGACTTCTTCAACTCCTCCAAGGAAATCTCCTTTTCTTTTGTCAGAAGGGTCTGGTTTTCATGGTGGTGCTGGTGTTCATTCAAGTAATAGGATGTCCCTTGAGACAGATTCTAATGGCTCTTCAACTGTTACTTGTCACAAATGTGGAGAACAGTTTAGCAAATGGGAAGCTGCTGAAACTCACCATCTCTCCAAGCATGCTGTTACTGAACTTGTCGAAGGAGATTCATCAAGGAAAATAGTAGAGATAATATGCAGAACAAGTTGGTTAAAGTCTGAAAACCACTGTGGTAGAATTGAGAGAGTTTTGAAGGTGCACAACATGCAAAAGACATTAGCTAGATTCGAAGAGTACCGAGAGATGGTGAAAATCAAAGCAAGCAAGCTCCAGAAGAAGCATCCTAGGTGTTTAGCGGACGGAAACGAGCTTTTACGGTTCTATGGAACCAGTGTTGCTTGTTCTCTTGGTCTCAATGGTTCGTCAAGTCTTTGCCTCTCAGAAAAATGCTGTGTTTGTAGAATTATCAGGAATGGGTTTTCTGCTAAGAAGGAGCTGAAAGGTGGTATAGGTGTTTTCACAACTTCCACAAGTGGAAGAGCTTTCGAGTCTATAGAGATTCTTGATAATGAACCATCACTAAGAAAGGCGTTGATAGTGTGCAGAGTAATCGCTGGCAGGGTTCATAGGCCGCTCGAGAACATTCAGGAAATGGCAGCTCAAACTGGATTTGATTCATTGGCTGGAAAAGTCGGTCTTTACTCGAATATCGAAGAGCTCTATTTGCTTAATCCTAGAGCTCTTCTTCCTTGTTTTGTCGTAATTTGCAAACCATGAACCCGAATAAAGATACGCGTCTTCGCACATGTTTGGATTTACAATGAATTTGACAGAATCACGGTAACACTCACGGCGATTTCCGGCATGAACTTACATATTGTGTAGTTTTATGAACGCTTACCACTCTTCTATTTTGGCCTTTTTCTATGGAGGTTCTTTGCTTTACATATTTTGAAGAATTTTCATTCTTTTTCACTTGTGCTTGTTCATGTTAATTGTACTAATATTTACTATGAACCACCTATTCATACATTTGTATTTTTHAACTTYTT
TARGET=609,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00643 Ps_contig_mira-and-tgicl-ass_franssen_6285
SEQUENCE=CCTTTTTTTAATTGGTAAGGAACTGGTGATCAATTGAAAAAGAAAAGAAAAATCTGTAAAAATTTAAATACTATCATTTATATGATTACATTGCATGGCCCTATTTTAGCCGTATACATGTCCAAAATCCTTACAGAATATAGACGAGAAATCCTGACATCGCCCTCTTCCTTAACTCCCTTAGTTTTGTTTCACCTTCATGGCTGGTATATTCTTTGCAAAAACTCACTGCTTTAATTTCTTCAAACCTTCCTTTTTGTCACGGCTTCGAATCAGTTGCGGTTTTAGGAGAGGGTGTTTTAATTTTACCACTACCGGAGACAACCTCGTCCATGCTTTGTCCCTGTCGTCTGTATCTACCACCAGAAGCAACAAATCCAGGGCATGGTCTGTAAGCTTTTATAGGCCACCATCCAAAGCCAGGCACAGTTGCTATCCCACCTTGCATCCTTCCTTCACCATTGCATCCTCTCTCTATTTCTTGCTTCCCACATGCTTTACAACCACTTTGTTGGGTTGAGGATTTTGATTGATTGGGAACAGCAAGTTGTTCTTCTGGTGAAGAAGAAAGGAGGGAAGTTGGAGTAGAAGAAGGAGAACATTGCGAGGGAGGAACAAGAATCTTAGAAAAACAAGGGGAAGTTGTAACCGACCATGGATTTTTTGGCTTGTGCAATTGAACTTGATGATAATGTTGAGTAGTTGAAACTGAGAGAAAAGCTATTGTCATTCTTTCTCTCTCCCTCGCTCCCCCTCTCTCTCTATTTGGGACAGAGTCCCCCGAGCCACTCTTTTAATTCTACTTATCCTMMMKWCC
TARGET=754,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00644 Ps_contig_mira-and-tgicl-ass_franssen_6298
SEQUENCE=GGGGATATATTTACCTTTTCGGGGATAAACCCGTACTGTATACTTCCTTCCTCGCTAAGTCCGCAGCACTAGTTTGTTTGTTTGATTTCCTCGCCGTCATGGTAGCTAAACCCCTTACCACCGAACTCGTTGCTCTTACAGAGAAGAAGATGGACATGACATTAGATGATATAATCAAAATGTCTAAAAACCCTAAAGTTACAAAGCAAACTAGGGTTTCGAACAAGAGCAAAAAATTTACAAACACTTTTGCACAAGATAAATCTTTGAAGGCGCGGCGATATATGGAGTCGAGATCTTCTCTTAGACAAGGAGTTCTTGCAAAAAGAAGGTCAAGTTTTCAAGGAAATCAATTTCCCCTTGCAACTGAGGTTGCGCGTAAAGTTGTTGCTGCTCCTCTGCATTTATGGAGTTGCTAATCGAAATAACATGGCTAACTGGAAATAAAACAAGGTATTGTTGTTTTTCTTTGATTGCTATTTGA
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4671
PRIMER_LEFT_PENALTY=0.389044
PRIMER_RIGHT_PENALTY=0.078029
PRIMER_LEFT_SEQUENCE=TTTACCTTTTCGGGGATAAACC
PRIMER_RIGHT_SEQUENCE=GATCTCGACTCCATATATCGCC
PRIMER_LEFT=9,22
PRIMER_RIGHT=297,22
PRIMER_LEFT_TM=60.389
PRIMER_RIGHT_TM=59.922
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.5513
PRIMER_LEFT_1_PENALTY=0.389044
PRIMER_RIGHT_1_PENALTY=0.162217
PRIMER_LEFT_1_SEQUENCE=TTTACCTTTTCGGGGATAAACC
PRIMER_RIGHT_1_SEQUENCE=TTGTTCGAAACCCTAGTTTGCT
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=225,22
PRIMER_LEFT_1_TM=60.389
PRIMER_RIGHT_1_TM=60.162
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.5513
PRIMER_LEFT_2_PENALTY=0.389044
PRIMER_RIGHT_2_PENALTY=0.162217
PRIMER_LEFT_2_SEQUENCE=TTTACCTTTTCGGGGATAAACC
PRIMER_RIGHT_2_SEQUENCE=TCGAAACCCTAGTTTGCTTTGT
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=221,22
PRIMER_LEFT_2_TM=60.389
PRIMER_RIGHT_2_TM=60.162
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=213
PRIMER_PAIR_PENALTY_3=0.5513
PRIMER_LEFT_3_PENALTY=0.389044
PRIMER_RIGHT_3_PENALTY=0.162217
PRIMER_LEFT_3_SEQUENCE=TTTACCTTTTCGGGGATAAACC
PRIMER_RIGHT_3_SEQUENCE=TGTTCGAAACCCTAGTTTGCTT
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=224,22
PRIMER_LEFT_3_TM=60.389
PRIMER_RIGHT_3_TM=60.162
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=216
PRIMER_PAIR_PENALTY_4=0.5513
PRIMER_LEFT_4_PENALTY=0.389044
PRIMER_RIGHT_4_PENALTY=0.162217
PRIMER_LEFT_4_SEQUENCE=TTTACCTTTTCGGGGATAAACC
PRIMER_RIGHT_4_SEQUENCE=CTTGTTCGAAACCCTAGTTTGC
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=226,22
PRIMER_LEFT_4_TM=60.389
PRIMER_RIGHT_4_TM=60.162
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_00645 Ps_contig_mira-and-tgicl-ass_franssen_6313
SEQUENCE=CTCTTTGTTCCTTAACCTTAAAAGAATAGACAAAGCTTCGTGTATTATCTGAGTCTATCAAATACGTGAAAGAGCTTAAAGAGCGTTTGGAAGTTTTGGAAGATCAGAACAAGAAAACAAAACTAGAATCGGTTGTTGTTCCGAACAAACCAGACATATGTAGCGATGAAGATAACTCTTCATGCGATGAGAACATTGAAGGTGGTGCTGGTGCTGGTGATAATGAATCACTCTTCCAAGTGGAAGCAAGAGAGTTAGGAAAAGAGATTCTGATTAGGATCCAGAGCAAGAAGAAGAAGGGTGTTTTGGTAAAAATGATGGAAGAGATTCAAAGTTTAGAATTATTTGTTGTGAATAGCAGTGTGTTACCTCTTGGGGATTCTGTCATTGACATTACGATCATTGCTCAGATGGGTGAAGGATACAGATTAAGCGTAAAGGAACTTGCGAAGAAGTTGCGNATAGAAAGCTATTAGTAAGTTTTATGTCATCAATTAATTAACGG
TARGET=288,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1907
PRIMER_LEFT_PENALTY=0.118120
PRIMER_RIGHT_PENALTY=0.072552
PRIMER_LEFT_SEQUENCE=AACTCTTCATGCGATGAGAACA
PRIMER_RIGHT_SEQUENCE=CAACTTCTTCGCAAGTTCCTTT
PRIMER_LEFT=173,22
PRIMER_RIGHT=457,22
PRIMER_LEFT_TM=59.882
PRIMER_RIGHT_TM=59.927
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.2541
PRIMER_LEFT_1_PENALTY=0.118120
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=AACTCTTCATGCGATGAGAACA
PRIMER_RIGHT_1_SEQUENCE=AGAATCCCCAAGAGGTAACACA
PRIMER_LEFT_1=173,22
PRIMER_RIGHT_1=382,22
PRIMER_LEFT_1_TM=59.882
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.2541
PRIMER_LEFT_2_PENALTY=0.118120
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=AACTCTTCATGCGATGAGAACA
PRIMER_RIGHT_2_SEQUENCE=ACAGAATCCCCAAGAGGTAACA
PRIMER_LEFT_2=173,22
PRIMER_RIGHT_2=384,22
PRIMER_LEFT_2_TM=59.882
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.2541
PRIMER_LEFT_3_PENALTY=0.118120
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=AACTCTTCATGCGATGAGAACA
PRIMER_RIGHT_3_SEQUENCE=CAGAATCCCCAAGAGGTAACAC
PRIMER_LEFT_3=173,22
PRIMER_RIGHT_3=383,22
PRIMER_LEFT_3_TM=59.882
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=0.2915
PRIMER_LEFT_4_PENALTY=0.118120
PRIMER_RIGHT_4_PENALTY=0.173354
PRIMER_LEFT_4_SEQUENCE=AACTCTTCATGCGATGAGAACA
PRIMER_RIGHT_4_SEQUENCE=ATGACAGAATCCCCAAGAGGTA
PRIMER_LEFT_4=173,22
PRIMER_RIGHT_4=387,22
PRIMER_LEFT_4_TM=59.882
PRIMER_RIGHT_4_TM=59.827
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_00646 Ps_contig_mira-and-tgicl-ass_franssen_6316
SEQUENCE=CACCGTTTGTTGTATCCATGGGATCAGCTTTCGGCTTCCCCTTCAGTAACTTTCTTACCTTTCTTCTTCTTCCCACCGCCCTTCTTCTTTGTCTTGACACCCAAGGCTAACCAGGCTTTAATCTCAGGATCATCTACTGTTTTAGTAGGCTGAATTTCCTGGAGTGGGTGAGATGTGATACGATCTGAACCATTTGGCATAAGCAAGACTGTGAATTTGATATGGGCAACATAATCACCAGATTTTTCATGTAGAACTGGATATGGTTGCAGAAGCTCATGATTCATGCATTCCACTAAACCCAGACGAGCTTCTTTTTTCTCCMAAGCCCTGGCAGAGAATGGCATGATAGGGTAAAATTTGGCTTAATTTCACTAGTAAATGAACCTAGATTCGTTCATTCTTACTAAGTTATTAACTTCTATCAACAGTCTCCTTACTTTAATAAAAGTTAGTTTCGG
TARGET=61,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00647 Ps_contig_mira-and-tgicl-ass_franssen_6321
SEQUENCE=CCCTAAGAACTTTTTGTTTGGAACTGCTTCTTCTTCTTACCAGGTGGTTATAACATTGATGGCAAGGGACAGAGTAATTGGGATAACTTCACTCATGGAGGTAGAAGTATGATAGTGGATGGAAGTAATGGGGATATTGCAGTTGACCATTATCATCGTTTCCAGGAGGATATAGATCTATTGGAAGCTTTGAAAGTAAATAGTTACCGACTCTCCATATCATGGACAAGATTCTACAAGGTGTTGANAATNATGC
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00648 Ps_contig_mira-and-tgicl-ass_franssen_6324
SEQUENCE=TCGGGGTATAAGTGGGAGCTTCGAACAGGGACTCCGGCTAAGTCGTCGTCAAAGCCGCCGACAAAAACCGGAACTTCACAGAAGCCGCAACCGAGACCAAATAATACTGGATCTTCACAGAAGCCGCAATCGAGACCAAATAATACTGGATCTTCACAGAAGCCGCAACAGAAATCAAATAACGGAGAAATTAAAGCAAAGCGAAAAATCGAGAGTGACAGTGATGATGACGACGACGACGATTTCATTTTGAGTCATAAAAGAAGAAGAGTGTAAGAAGAAAAACCTCACAAATTTTATTTAATAAAGGTTATAAAAAGGAAAAGAAAAGATTTGTTGTAAGGTAACTTGAACTGTAAGATTAAAATATTCTTCATGTGTCAAACTTTACTGATTCAACAAGGGACTTGCACTGTGTTGTTGTATGTGTCTTTTGCAAGCAACATTATTAATGAAGATGGAGAGTGCTTTTACTCCATTACTTCTTAAACC
TARGET=229,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6233
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.536975
PRIMER_LEFT_SEQUENCE=CGAAAAATCGAGAGTGACAGTG
PRIMER_RIGHT_SEQUENCE=GCAAGTCCCTTGTTGAATCAGT
PRIMER_LEFT=201,22
PRIMER_RIGHT=410,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=60.537
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.6233
PRIMER_LEFT_1_PENALTY=0.086339
PRIMER_RIGHT_1_PENALTY=0.536975
PRIMER_LEFT_1_SEQUENCE=CGAAAAATCGAGAGTGACAGTG
PRIMER_RIGHT_1_SEQUENCE=AGTGCAAGTCCCTTGTTGAATC
PRIMER_LEFT_1=201,22
PRIMER_RIGHT_1=413,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=60.537
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.6387
PRIMER_LEFT_2_PENALTY=0.101680
PRIMER_RIGHT_2_PENALTY=0.536975
PRIMER_LEFT_2_SEQUENCE=AAAGCGAAAAATCGAGAGTGAC
PRIMER_RIGHT_2_SEQUENCE=GCAAGTCCCTTGTTGAATCAGT
PRIMER_LEFT_2=197,22
PRIMER_RIGHT_2=410,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.537
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.6387
PRIMER_LEFT_3_PENALTY=0.101680
PRIMER_RIGHT_3_PENALTY=0.536975
PRIMER_LEFT_3_SEQUENCE=AAAGCGAAAAATCGAGAGTGAC
PRIMER_RIGHT_3_SEQUENCE=AGTGCAAGTCCCTTGTTGAATC
PRIMER_LEFT_3=197,22
PRIMER_RIGHT_3=413,22
PRIMER_LEFT_3_TM=59.898
PRIMER_RIGHT_3_TM=60.537
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=1.0857
PRIMER_LEFT_4_PENALTY=0.086339
PRIMER_RIGHT_4_PENALTY=0.999370
PRIMER_LEFT_4_SEQUENCE=CGAAAAATCGAGAGTGACAGTG
PRIMER_RIGHT_4_SEQUENCE=AACAACACAGTGCAAGTCCCTT
PRIMER_LEFT_4=201,22
PRIMER_RIGHT_4=421,22
PRIMER_LEFT_4_TM=59.914
PRIMER_RIGHT_4_TM=60.999
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_00649 Ps_contig_mira-and-tgicl-ass_franssen_6325
SEQUENCE=ACTCATAGAAATCCTCAAGGGTTCTTTTCTCTTTTCAGGTGGATCGTGTTTCTGGCCAGGCAAATTATAGACTTTCCTCTCTCTCACTTTGGTGGTAGTAGTTTTCCTTCTTTGTCGTCTTCGTAGTCGTCGTTGTTTTCGATGATGACGACAACATTGTTGTCGGCTTTGATTTAACCTCAGATTTCGTGAGAGTGGTGACAGATTTCATCTTATTTGAGCTGGGGAGATTCTTATTGAGAGAAGAAGAAGAATCAGTAATCTTAGGCTTGGAAGGGGCAATTTGAGAAATTGGATTGATCTTCACTGGAGTGGTTTTGGTCTCAGCCATGTCTTTGAAAGTAACTGTTATGTACTGTATCTATAGATTATTGAAGTCCCCC
TARGET=242,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2039
PRIMER_LEFT_PENALTY=0.092792
PRIMER_RIGHT_PENALTY=0.111077
PRIMER_LEFT_SEQUENCE=CTCAGATTTCGTGAGAGTGGTG
PRIMER_RIGHT_SEQUENCE=CACTCCAGTGAAGATCAATCCA
PRIMER_LEFT=178,22
PRIMER_RIGHT=313,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.111
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=0.3423
PRIMER_LEFT_1_PENALTY=0.231272
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=ATTTCGTGAGAGTGGTGACAGA
PRIMER_RIGHT_1_SEQUENCE=CACTCCAGTGAAGATCAATCCA
PRIMER_LEFT_1=183,22
PRIMER_RIGHT_1=313,22
PRIMER_LEFT_1_TM=59.769
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.3423
PRIMER_LEFT_2_PENALTY=0.231272
PRIMER_RIGHT_2_PENALTY=0.111077
PRIMER_LEFT_2_SEQUENCE=GATTTCGTGAGAGTGGTGACAG
PRIMER_RIGHT_2_SEQUENCE=CACTCCAGTGAAGATCAATCCA
PRIMER_LEFT_2=182,22
PRIMER_RIGHT_2=313,22
PRIMER_LEFT_2_TM=59.769
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=0.3423
PRIMER_LEFT_3_PENALTY=0.231272
PRIMER_RIGHT_3_PENALTY=0.111077
PRIMER_LEFT_3_SEQUENCE=TTCGTGAGAGTGGTGACAGATT
PRIMER_RIGHT_3_SEQUENCE=CACTCCAGTGAAGATCAATCCA
PRIMER_LEFT_3=185,22
PRIMER_RIGHT_3=313,22
PRIMER_LEFT_3_TM=59.769
PRIMER_RIGHT_3_TM=60.111
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.3423
PRIMER_LEFT_4_PENALTY=0.231272
PRIMER_RIGHT_4_PENALTY=0.111077
PRIMER_LEFT_4_SEQUENCE=TCGTGAGAGTGGTGACAGATTT
PRIMER_RIGHT_4_SEQUENCE=CACTCCAGTGAAGATCAATCCA
PRIMER_LEFT_4=186,22
PRIMER_RIGHT_4=313,22
PRIMER_LEFT_4_TM=59.769
PRIMER_RIGHT_4_TM=60.111
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_00650 Ps_contig_mira-and-tgicl-ass_franssen_6330
SEQUENCE=CGTTTTATTAAGAACCAAATGAATGTCTTATTTATCCTCATCTTTTGTAATTAAAGCAAAATGGAACATTATTTGAATTTTACAAGTAAATGATGAATGTAAATACAGATGCACCCCAAATTTTGGTGTGCATTATGTTTGTTTGGCTATTTGTTGGTTTCCCTCAAAATACAACAGTCTTTTTGCCTTCATAAGGTAATACTCTAAGTTCACAATAAGATCTTATAGCCTTGGAAAGACATTGTTTCTCGAGGTTTTCTGATTTCTGCACAAAGCTCTGCAAGTTATCTCTGTGTGAGACCCTCTCGACCATTTGTTCAATTATAGGTCCGGCGTCAAGTTCTTCGGTCACAAAGTGAGTTGTTGCTCCAATTAATTTCACACCAGCCTCAAAGGCCTGTTTCGATGGATGACCACCCTTGAAAGATGGCAACAAACCATGGTGAATGTTAATTACGTCGTTACCGTAGCTCCTTAGAAAGTTTCCAGATAGTATCTGCATATATCTTGCAAGTACTAAAAAATCGGTATTCTGAACAAGCTCTAATATCTCTCTTTCTCTTTTATTTTCTTTGGTCATGCTTAAACAATGATATGGAATACCGTGCCTTTCGAGAAAACGAATCACATGTGTGTTTGAATCTCTATGATGGTTACTGATTACACAAGTGATATCCACCGGAAGTCTCCCGTCCTGCCATCCGTGTAATAAATCAACCAAACAATGGTCCTGCTTTGATGCAAGAACGGCTATTTTATATTTAGGATCTAGAGCCGGCACTCTCACGACTGATCTCGTTGCATTGAATGCCTGTGAAAGCTTTAGGAAATCCTCCTCCATCTGCATCCGTGGCCATTTAAGAGGATCAAAAACAAAATCACTTCTAGAGTAAAAGACAAGTTTATTTTGAGGAACAAAAACATCAGCAGACAAAATGTTTCCACCTTTAGAGGCAATACAATCAGATAACTTTGCAACAATTCCAACGGTATCGGGACAATGAAAAACGTGGATTCCATGGCAGAGTGATGGTAATGGTGGATCTAAGGATTTGAGTGAGAAATTCCTGATCTTAGTGTTGTTGGATAATCCCAAAACTTGGGATACTCTTCTTACCACCATGCCCATTTTTTCTCTCTCTCTCTCTCTGAACCACAAGTTACACTACATGATTCAAGAGGATCAAGTGTTAGGAGC
TARGET=1134,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5765
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=1.575769
PRIMER_LEFT_SEQUENCE=TGTTTCCACCTTTAGAGGCAAT
PRIMER_RIGHT_SEQUENCE=GCTCCTAACACTTGATCCTCTTG
PRIMER_LEFT=936,22
PRIMER_RIGHT=1197,23
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.424
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=1.5765
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=1.575769
PRIMER_LEFT_1_SEQUENCE=AATGTTTCCACCTTTAGAGGCA
PRIMER_RIGHT_1_SEQUENCE=GCTCCTAACACTTGATCCTCTTG
PRIMER_LEFT_1=934,22
PRIMER_RIGHT_1=1197,23
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.424
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=1.5765
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=1.575769
PRIMER_LEFT_2_SEQUENCE=ATGTTTCCACCTTTAGAGGCAA
PRIMER_RIGHT_2_SEQUENCE=GCTCCTAACACTTGATCCTCTTG
PRIMER_LEFT_2=935,22
PRIMER_RIGHT_2=1197,23
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=59.424
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=1.6156
PRIMER_LEFT_3_PENALTY=0.039879
PRIMER_RIGHT_3_PENALTY=1.575769
PRIMER_LEFT_3_SEQUENCE=AGCAGACAAAATGTTTCCACCT
PRIMER_RIGHT_3_SEQUENCE=GCTCCTAACACTTGATCCTCTTG
PRIMER_LEFT_3=925,22
PRIMER_RIGHT_3=1197,23
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=59.424
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=1.7298
PRIMER_LEFT_4_PENALTY=0.154023
PRIMER_RIGHT_4_PENALTY=1.575769
PRIMER_LEFT_4_SEQUENCE=GGAACAAAAACATCAGCAGACA
PRIMER_RIGHT_4_SEQUENCE=GCTCCTAACACTTGATCCTCTTG
PRIMER_LEFT_4=911,22
PRIMER_RIGHT_4=1197,23
PRIMER_LEFT_4_TM=60.154
PRIMER_RIGHT_4_TM=59.424
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_00651 Ps_contig_mira-and-tgicl-ass_franssen_6334
SEQUENCE=CTTTTTCTATTTTATTTTCTCGCTTGTCAATTTCCAAGGCATCTCCACCACTCTTTTTTCTAATATATCATCATCTTCCTCGTTTAATCTACCTTTACTTATTTTATTTATTTCCTCTATCTTTTGCAGTTTCATGTCACTTTTAGATGAGCTGAACGCATAGATACAATTATTATTATTATTATTATTATATGTATGTATGGCAACGTTGGGTGCAAACTCAAGTATTATTATTAAAAATAGATTTAAGGTTTCATACAACAACAACAACAT
TARGET=169,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7793
PRIMER_LEFT_PENALTY=0.482784
PRIMER_RIGHT_PENALTY=1.296491
PRIMER_LEFT_SEQUENCE=CTTGTCAATTTCCAAGGCATCT
PRIMER_RIGHT_SEQUENCE=ATACTTGAGTTTGCACCCAACG
PRIMER_LEFT=22,22
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=60.483
PRIMER_RIGHT_TM=61.296
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=1.8541
PRIMER_LEFT_1_PENALTY=0.482784
PRIMER_RIGHT_1_PENALTY=1.371364
PRIMER_LEFT_1_SEQUENCE=CTTGTCAATTTCCAAGGCATCT
PRIMER_RIGHT_1_SEQUENCE=ACCCAACGTTGCCATACATAC
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=213,21
PRIMER_LEFT_1_TM=60.483
PRIMER_RIGHT_1_TM=59.629
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=2.1029
PRIMER_LEFT_2_PENALTY=0.482784
PRIMER_RIGHT_2_PENALTY=1.620131
PRIMER_LEFT_2_SEQUENCE=CTTGTCAATTTCCAAGGCATCT
PRIMER_RIGHT_2_SEQUENCE=CCAACGTTGCCATACATACATA
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=60.483
PRIMER_RIGHT_2_TM=58.380
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=2.2240
PRIMER_LEFT_3_PENALTY=0.482784
PRIMER_RIGHT_3_PENALTY=1.741252
PRIMER_LEFT_3_SEQUENCE=CTTGTCAATTTCCAAGGCATCT
PRIMER_RIGHT_3_SEQUENCE=CCCAACGTTGCCATACATACAT
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=212,22
PRIMER_LEFT_3_TM=60.483
PRIMER_RIGHT_3_TM=61.741
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=2.3079
PRIMER_LEFT_4_PENALTY=0.482784
PRIMER_RIGHT_4_PENALTY=1.825088
PRIMER_LEFT_4_SEQUENCE=CTTGTCAATTTCCAAGGCATCT
PRIMER_RIGHT_4_SEQUENCE=AATACTTGAGTTTGCACCCAAC
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=228,22
PRIMER_LEFT_4_TM=60.483
PRIMER_RIGHT_4_TM=58.175
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_00652 Ps_contig_mira-and-tgicl-ass_franssen_6334
SEQUENCE=CTTTTTCTATTTTATTTTCTCGCTTGTCAATTTCCAAGGCATCTCCACCACTCTTTTTTCTAATATATCATCATCTTCCTCGTTTAATCTACCTTTACTTATTTTATTTATTTCCTCTATCTTTTGCAGTTTCATGTCACTTTTAGATGAGCTGAACGCATAGATACAATTATTATTATTATTATTATTATATGTATGTATGGCAACGTTGGGTGCAAACTCAAGTATTATTATTAAAAATAGATTTAAGGTTTCATACAACAACAACAACAT
TARGET=191,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7793
PRIMER_LEFT_PENALTY=0.482784
PRIMER_RIGHT_PENALTY=1.296491
PRIMER_LEFT_SEQUENCE=CTTGTCAATTTCCAAGGCATCT
PRIMER_RIGHT_SEQUENCE=ATACTTGAGTTTGCACCCAACG
PRIMER_LEFT=22,22
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=60.483
PRIMER_RIGHT_TM=61.296
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=2.3079
PRIMER_LEFT_1_PENALTY=0.482784
PRIMER_RIGHT_1_PENALTY=1.825088
PRIMER_LEFT_1_SEQUENCE=CTTGTCAATTTCCAAGGCATCT
PRIMER_RIGHT_1_SEQUENCE=AATACTTGAGTTTGCACCCAAC
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=228,22
PRIMER_LEFT_1_TM=60.483
PRIMER_RIGHT_1_TM=58.175
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=2.3626
PRIMER_LEFT_2_PENALTY=1.066143
PRIMER_RIGHT_2_PENALTY=1.296491
PRIMER_LEFT_2_SEQUENCE=TGTCAATTTCCAAGGCATCTC
PRIMER_RIGHT_2_SEQUENCE=ATACTTGAGTTTGCACCCAACG
PRIMER_LEFT_2=24,21
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=60.066
PRIMER_RIGHT_2_TM=61.296
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=204
PRIMER_PAIR_PENALTY_3=2.4211
PRIMER_LEFT_3_PENALTY=1.124598
PRIMER_RIGHT_3_PENALTY=1.296491
PRIMER_LEFT_3_SEQUENCE=AGGCATCTCCACCACTCTTTT
PRIMER_RIGHT_3_SEQUENCE=ATACTTGAGTTTGCACCCAACG
PRIMER_LEFT_3=36,21
PRIMER_RIGHT_3=227,22
PRIMER_LEFT_3_TM=60.125
PRIMER_RIGHT_3_TM=61.296
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=2.4211
PRIMER_LEFT_4_PENALTY=1.124598
PRIMER_RIGHT_4_PENALTY=1.296491
PRIMER_LEFT_4_SEQUENCE=AAGGCATCTCCACCACTCTTT
PRIMER_RIGHT_4_SEQUENCE=ATACTTGAGTTTGCACCCAACG
PRIMER_LEFT_4=35,21
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=60.125
PRIMER_RIGHT_4_TM=61.296
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_00653 Ps_contig_mira-and-tgicl-ass_franssen_6334
SEQUENCE=CTTTTTCTATTTTATTTTCTCGCTTGTCAATTTCCAAGGCATCTCCACCACTCTTTTTTCTAATATATCATCATCTTCCTCGTTTAATCTACCTTTACTTATTTTATTTATTTCCTCTATCTTTTGCAGTTTCATGTCACTTTTAGATGAGCTGAACGCATAGATACAATTATTATTATTATTATTATTATATGTATGTATGGCAACGTTGGGTGCAAACTCAAGTATTATTATTAAAAATAGATTTAAGGTTTCATACAACAACAACAACAT
TARGET=258,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00654 Ps_contig_mira-and-tgicl-ass_franssen_6366
SEQUENCE=TAAGACGTGCCCGATAGTGCCGCAACTATTATTCGCAGAGCGCTTGCCTGTATATGATGTCTAGGTGTTAACTTGACCAACTTTTGTATACAAGACCATAGAAGAAAGTGACTAAAGGAAAGTGAAAAAATGTTAATACTTGGTATATTTTTTGTTGTCACATTAATTCAGTGCCTAACTTTTTTTTTACAATTTGTATATATATAATAGATATTTTGAGGGTTTTACAATTCAGTCCACCCAATTTTTANCCGGGTCAATCAAGTGTAAAAACTTGGCTTCAATTTA
TARGET=197,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6563
PRIMER_LEFT_PENALTY=0.271198
PRIMER_RIGHT_PENALTY=0.385098
PRIMER_LEFT_SEQUENCE=AGAGCGCTTGCCTGTATATGAT
PRIMER_RIGHT_SEQUENCE=GGGTGGACTGAATTGTAAAACC
PRIMER_LEFT=36,22
PRIMER_RIGHT=241,22
PRIMER_LEFT_TM=60.271
PRIMER_RIGHT_TM=59.615
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.6563
PRIMER_LEFT_1_PENALTY=0.271198
PRIMER_RIGHT_1_PENALTY=0.385098
PRIMER_LEFT_1_SEQUENCE=AGAGCGCTTGCCTGTATATGAT
PRIMER_RIGHT_1_SEQUENCE=GGTGGACTGAATTGTAAAACCC
PRIMER_LEFT_1=36,22
PRIMER_RIGHT_1=240,22
PRIMER_LEFT_1_TM=60.271
PRIMER_RIGHT_1_TM=59.615
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=1.1103
PRIMER_LEFT_2_PENALTY=0.725159
PRIMER_RIGHT_2_PENALTY=0.385098
PRIMER_LEFT_2_SEQUENCE=CGCTTGCCTGTATATGATGTCT
PRIMER_RIGHT_2_SEQUENCE=GGGTGGACTGAATTGTAAAACC
PRIMER_LEFT_2=40,22
PRIMER_RIGHT_2=241,22
PRIMER_LEFT_2_TM=59.275
PRIMER_RIGHT_2_TM=59.615
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=1.1103
PRIMER_LEFT_3_PENALTY=0.725159
PRIMER_RIGHT_3_PENALTY=0.385098
PRIMER_LEFT_3_SEQUENCE=CGCTTGCCTGTATATGATGTCT
PRIMER_RIGHT_3_SEQUENCE=GGTGGACTGAATTGTAAAACCC
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=240,22
PRIMER_LEFT_3_TM=59.275
PRIMER_RIGHT_3_TM=59.615
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=1.3524
PRIMER_LEFT_4_PENALTY=0.271198
PRIMER_RIGHT_4_PENALTY=1.081208
PRIMER_LEFT_4_SEQUENCE=AGAGCGCTTGCCTGTATATGAT
PRIMER_RIGHT_4_SEQUENCE=GGNTAAAAATTGGGTGGACTGA
PRIMER_LEFT_4=36,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=60.271
PRIMER_RIGHT_4_TM=61.081
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_00655 Ps_contig_mira-and-tgicl-ass_franssen_6374
SEQUENCE=TGTCGTTCATCATGACCTCCGTAACCGTTTTCCGTTTTTCCTATTACGTGCACCGGTTTYCGAAGGGAGACTGAGCGTAACTTGAATCCTTGATCGCTGAATCCGAATATTTCAGGCGACTCCGCCGCTGCTGCTGGTGTGTGATCTACGGGGACTTCTTCGCCGGAGAAGCTGGAGAATCCGCCGTATTCGGAGTAAGTGTCGTCACCGGCAGCGAAGTGGTGTTCGTCGACGGTGAATGACATGGCTGTGATTTGCGGTGTCTCTAGTTTCTAATAAATAAATAAACGGTTGAACGATGGAAGAAGTTGAAGTAGGAAAAG
TARGET=275,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00656 Ps_contig_mira-and-tgicl-ass_franssen_6390
SEQUENCE=CAACACAAAGTGTTGGATATTGTGTTGTGCTACATGAGGATTTGATGAAGTTTGTTTGGTTGTTGATGTTGCTATAGAGACAAGAGTTATTAGAAGGAAAAGAAGGGAAACCATGGTTGCCATGTTTGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTAGTGTTGTTGTTGTGTGT
TARGET=129,46
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00657 Ps_contig_mira-and-tgicl-ass_franssen_6390
SEQUENCE=CAACACAAAGTGTTGGATATTGTGTTGTGCTACATGAGGATTTGATGAAGTTTGTTTGGTTGTTGATGTTGCTATAGAGACAAGAGTTATTAGAAGGAAAAGAAGGGAAACCATGGTTGCCATGTTTGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTAGTGTTGTTGTTGTGTGT
TARGET=179,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00658 Ps_contig_mira-and-tgicl-ass_franssen_6411
SEQUENCE=GGTCCCATCACCACCCAATCTCTCTCTCTTTTCCTCTCCATAATACCAATCCCTCTCTSACGCATATTACCAAAAAGTCACCGWCATAATGGAAGCTGCATCGGGTGGAGAACCACCACCGCCACCGTCAGCACAACCTCAACCGGACTCATCACCACCAACACCAAGCCGATACGAGTCACAAAAGCGACGAGACTGGAACACGTTCCAACAGTATCTTCCAAATCACAAACCTCCATTAACCCTAGCACGTTGCAGCGGAGCGCACGTGATTGTAGTTTTTAGAAATTA
TARGET=19,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00659 Ps_contig_mira-and-tgicl-ass_franssen_6420
SEQUENCE=TAAAGCCTTACGTCTTTGTTCAAACGACGACAGAAGAGGGAAGAATCAATCGAGGGTTTCATTTCTCCTCCTCCTCCTCCTCACAATTACCCTATGAATCAATCATCCACACTCTGAAAATCCCAAATTTCTCACTCGATGGATTCAAACTCACAGTCTCCGCCGTGTGGCGATTCTGACACCAAAAATGTATTTCGCAAGCCTTCTGGGGAGGCAGCTAATAGGAATTATCGGCGCCGATCACCTATTGATGGGTCATCATCACCTGATGGTAAGTCCCAGGAGAGAACACAGCTCAAGTCC
TARGET=65,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4018
PRIMER_LEFT_PENALTY=0.180765
PRIMER_RIGHT_PENALTY=0.220992
PRIMER_LEFT_SEQUENCE=GTCTTTGTTCAAACGACGACAG
PRIMER_RIGHT_SEQUENCE=ATTTGGGATTTTCAGAGTGTGG
PRIMER_LEFT=11,22
PRIMER_RIGHT=127,22
PRIMER_LEFT_TM=59.819
PRIMER_RIGHT_TM=60.221
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=117
PRIMER_PAIR_PENALTY_1=0.4661
PRIMER_LEFT_1_PENALTY=0.200343
PRIMER_RIGHT_1_PENALTY=0.265715
PRIMER_LEFT_1_SEQUENCE=CAGAAGAGGGAAGAATCAATCG
PRIMER_RIGHT_1_SEQUENCE=CTGTGAGTTTGAATCCATCGAG
PRIMER_LEFT_1=30,22
PRIMER_RIGHT_1=155,22
PRIMER_LEFT_1_TM=60.200
PRIMER_RIGHT_1_TM=59.734
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.4677
PRIMER_LEFT_2_PENALTY=0.246726
PRIMER_RIGHT_2_PENALTY=0.220992
PRIMER_LEFT_2_SEQUENCE=ACGACAGAAGAGGGAAGAATCA
PRIMER_RIGHT_2_SEQUENCE=ATTTGGGATTTTCAGAGTGTGG
PRIMER_LEFT_2=26,22
PRIMER_RIGHT_2=127,22
PRIMER_LEFT_2_TM=60.247
PRIMER_RIGHT_2_TM=60.221
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=102
PRIMER_PAIR_PENALTY_3=0.5004
PRIMER_LEFT_3_PENALTY=0.180765
PRIMER_RIGHT_3_PENALTY=0.319597
PRIMER_LEFT_3_SEQUENCE=GTCTTTGTTCAAACGACGACAG
PRIMER_RIGHT_3_SEQUENCE=CATCGAGTGAGAAATTTGGGAT
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=140,22
PRIMER_LEFT_3_TM=59.819
PRIMER_RIGHT_3_TM=60.320
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=130
PRIMER_PAIR_PENALTY_4=0.5004
PRIMER_LEFT_4_PENALTY=0.180765
PRIMER_RIGHT_4_PENALTY=0.319597
PRIMER_LEFT_4_SEQUENCE=GTCTTTGTTCAAACGACGACAG
PRIMER_RIGHT_4_SEQUENCE=ATCCATCGAGTGAGAAATTTGG
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=143,22
PRIMER_LEFT_4_TM=59.819
PRIMER_RIGHT_4_TM=60.320
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_00660 Ps_contig_mira-and-tgicl-ass_franssen_6427
SEQUENCE=GGCACAAACCACAGACTTTACCATTTTCTTCTTCTTCTTCCCTACTTTTTATTTGAATTTTGTACAGCGAAACAACGAAGCTTCGCAATTCACCAACTCCATAATCATCGATTCGATTTCTCTTCTGTAAAGGAGGATGGCGTGTAGGGGATTCTGGGAGTGTCTTTTGAAGCTTTTCAACTTCGTTCTGAGTGTGACTGGTTTGGCCATGGTGGGTTATGGGATCTATTTGGTGGTTCAATTCAGTAAAGCTTCTGATGATGATACGCCGGCGTATTTCTCCAGCAAGTGATGATAGT
TARGET=26,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1520
PRIMER_LEFT_PENALTY=0.073989
PRIMER_RIGHT_PENALTY=0.078029
PRIMER_LEFT_SEQUENCE=GCACAAACCACAGACTTTACCA
PRIMER_RIGHT_SEQUENCE=AAGCTTCAAAAGACACTCCCAG
PRIMER_LEFT=1,22
PRIMER_RIGHT=174,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.922
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=0.2646
PRIMER_LEFT_1_PENALTY=0.073989
PRIMER_RIGHT_1_PENALTY=0.190632
PRIMER_LEFT_1_SEQUENCE=GCACAAACCACAGACTTTACCA
PRIMER_RIGHT_1_SEQUENCE=AACCAGTCACACTCAGAACGAA
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=202,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.809
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.2646
PRIMER_LEFT_2_PENALTY=0.073989
PRIMER_RIGHT_2_PENALTY=0.190632
PRIMER_LEFT_2_SEQUENCE=GCACAAACCACAGACTTTACCA
PRIMER_RIGHT_2_SEQUENCE=AAACCAGTCACACTCAGAACGA
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=203,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.809
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.3053
PRIMER_LEFT_3_PENALTY=0.073989
PRIMER_RIGHT_3_PENALTY=0.231323
PRIMER_LEFT_3_SEQUENCE=GCACAAACCACAGACTTTACCA
PRIMER_RIGHT_3_SEQUENCE=CATCACTTGCTGGAGAAATACG
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=293,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.769
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.3488
PRIMER_LEFT_4_PENALTY=0.073989
PRIMER_RIGHT_4_PENALTY=0.274768
PRIMER_LEFT_4_SEQUENCE=GCACAAACCACAGACTTTACCA
PRIMER_RIGHT_4_SEQUENCE=AAAGCTTCAAAAGACACTCCCA
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=175,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=60.275
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_00661 Ps_contig_mira-and-tgicl-ass_franssen_6430
SEQUENCE=CAACTTTGAAAGAAATGAGCTCTACAGCCCGTAGTACTTCATTGATAGCCCCAGATACCATGATAATCCTATCCGTAGTTCCAGGGAAGAATTCATTATTGCGTGACAACTGAATTCGTGCTCCAGATTGTGACTGAAAATCAGTGATAGTCGAACCACCCTTTCCAATGACAGAACCAGCAGCAGAGTTTGATACCAGAAACCTAACGTATGTTGGTTTTTCCGCAGAATCTTGAGAAGGTGATTCCGGAGGAGGTGAAGAAGCGTGCTTCCTCGGTCCTTCTGGAGATGAGACGTAAGAAGATTCAGTCGACTCCATTGTTGTTGTTGTTGTTGTTCGTCCTCTTCCTCTTGAGCAAAAGAACCTAATTCTCACTCCTTCGTTTCAAAGAGCGAGAGAAAGAGACGGTTAATTTCAGAGAGAAAGGTGAGAGAAAAATTTCATAGTAATGGCGTGAGGTGAGAAATGAAAGTGTTGAGAGAATTGAAAAAAGTGAAAACGGCGGAGAGGGAAGAGAGAGAGGGGGTTGAACCGGAGATGAGAGAGAAAGAGAGAGAGAGAGGAATGAGAAGAAGAGAAGAAGTTGCTGCTCTGAAGTTTTGGTTTGTTTCTGCTCTCTACAACGTCC
TARGET=319,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0763
PRIMER_LEFT_PENALTY=0.073989
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=CAGCAGCAGAGTTTGATACCAG
PRIMER_RIGHT_SEQUENCE=TTTTGCTCAAGAGGAAGAGGAC
PRIMER_LEFT=177,22
PRIMER_RIGHT=360,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.0768
PRIMER_LEFT_1_PENALTY=0.074466
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=GAATCTTGAGAAGGTGATTCCG
PRIMER_RIGHT_1_SEQUENCE=TTTTGCTCAAGAGGAAGAGGAC
PRIMER_LEFT_1=227,22
PRIMER_RIGHT_1=360,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=0.0918
PRIMER_LEFT_2_PENALTY=0.073989
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=CAGCAGCAGAGTTTGATACCAG
PRIMER_RIGHT_2_SEQUENCE=CACCTCACGCCATTACTATGAA
PRIMER_LEFT_2=177,22
PRIMER_RIGHT_2=461,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.0923
PRIMER_LEFT_3_PENALTY=0.074466
PRIMER_RIGHT_3_PENALTY=0.017846
PRIMER_LEFT_3_SEQUENCE=GAATCTTGAGAAGGTGATTCCG
PRIMER_RIGHT_3_SEQUENCE=CACCTCACGCCATTACTATGAA
PRIMER_LEFT_3=227,22
PRIMER_RIGHT_3=461,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.1321
PRIMER_LEFT_4_PENALTY=0.129818
PRIMER_RIGHT_4_PENALTY=0.002310
PRIMER_LEFT_4_SEQUENCE=GTGATAGTCGAACCACCCTTTC
PRIMER_RIGHT_4_SEQUENCE=TTTTGCTCAAGAGGAAGAGGAC
PRIMER_LEFT_4=143,22
PRIMER_RIGHT_4=360,22
PRIMER_LEFT_4_TM=59.870
PRIMER_RIGHT_4_TM=59.998
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_00662 Ps_contig_mira-and-tgicl-ass_franssen_6430
SEQUENCE=CAACTTTGAAAGAAATGAGCTCTACAGCCCGTAGTACTTCATTGATAGCCCCAGATACCATGATAATCCTATCCGTAGTTCCAGGGAAGAATTCATTATTGCGTGACAACTGAATTCGTGCTCCAGATTGTGACTGAAAATCAGTGATAGTCGAACCACCCTTTCCAATGACAGAACCAGCAGCAGAGTTTGATACCAGAAACCTAACGTATGTTGGTTTTTCCGCAGAATCTTGAGAAGGTGATTCCGGAGGAGGTGAAGAAGCGTGCTTCCTCGGTCCTTCTGGAGATGAGACGTAAGAAGATTCAGTCGACTCCATTGTTGTTGTTGTTGTTGTTCGTCCTCTTCCTCTTGAGCAAAAGAACCTAATTCTCACTCCTTCGTTTCAAAGAGCGAGAGAAAGAGACGGTTAATTTCAGAGAGAAAGGTGAGAGAAAAATTTCATAGTAATGGCGTGAGGTGAGAAATGAAAGTGTTGAGAGAATTGAAAAAAGTGAAAACGGCGGAGAGGGAAGAGAGAGAGGGGGTTGAACCGGAGATGAGAGAGAAAGAGAGAGAGAGAGGAATGAGAAGAAGAGAAGAAGTTGCTGCTCTGAAGTTTTGGTTTGTTTCTGCTCTCTACAACGTCC
TARGET=514,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5566
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=0.538752
PRIMER_LEFT_SEQUENCE=TTCATAGTAATGGCGTGAGGTG
PRIMER_RIGHT_SEQUENCE=CTTTCTCTCTCATCTCCGGTTC
PRIMER_LEFT=440,22
PRIMER_RIGHT=550,22
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=59.461
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=111
PRIMER_PAIR_PENALTY_1=0.5566
PRIMER_LEFT_1_PENALTY=0.017846
PRIMER_RIGHT_1_PENALTY=0.538752
PRIMER_LEFT_1_SEQUENCE=TTCATAGTAATGGCGTGAGGTG
PRIMER_RIGHT_1_SEQUENCE=TCTTTCTCTCTCATCTCCGGTT
PRIMER_LEFT_1=440,22
PRIMER_RIGHT_1=551,22
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=59.461
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=112
PRIMER_PAIR_PENALTY_2=0.6029
PRIMER_LEFT_2_PENALTY=0.064105
PRIMER_RIGHT_2_PENALTY=0.538752
PRIMER_LEFT_2_SEQUENCE=CGACTCCATTGTTGTTGTTGTT
PRIMER_RIGHT_2_SEQUENCE=CTTTCTCTCTCATCTCCGGTTC
PRIMER_LEFT_2=310,22
PRIMER_RIGHT_2=550,22
PRIMER_LEFT_2_TM=59.936
PRIMER_RIGHT_2_TM=59.461
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=0.6029
PRIMER_LEFT_3_PENALTY=0.064105
PRIMER_RIGHT_3_PENALTY=0.538752
PRIMER_LEFT_3_SEQUENCE=CGACTCCATTGTTGTTGTTGTT
PRIMER_RIGHT_3_SEQUENCE=TCTTTCTCTCTCATCTCCGGTT
PRIMER_LEFT_3=310,22
PRIMER_RIGHT_3=551,22
PRIMER_LEFT_3_TM=59.936
PRIMER_RIGHT_3_TM=59.461
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.6121
PRIMER_LEFT_4_PENALTY=0.073355
PRIMER_RIGHT_4_PENALTY=0.538752
PRIMER_LEFT_4_SEQUENCE=CAGTCGACTCCATTGTTGTTGT
PRIMER_RIGHT_4_SEQUENCE=CTTTCTCTCTCATCTCCGGTTC
PRIMER_LEFT_4=306,22
PRIMER_RIGHT_4=550,22
PRIMER_LEFT_4_TM=60.073
PRIMER_RIGHT_4_TM=59.461
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_00663 Ps_contig_mira-and-tgicl-ass_franssen_6430
SEQUENCE=CAACTTTGAAAGAAATGAGCTCTACAGCCCGTAGTACTTCATTGATAGCCCCAGATACCATGATAATCCTATCCGTAGTTCCAGGGAAGAATTCATTATTGCGTGACAACTGAATTCGTGCTCCAGATTGTGACTGAAAATCAGTGATAGTCGAACCACCCTTTCCAATGACAGAACCAGCAGCAGAGTTTGATACCAGAAACCTAACGTATGTTGGTTTTTCCGCAGAATCTTGAGAAGGTGATTCCGGAGGAGGTGAAGAAGCGTGCTTCCTCGGTCCTTCTGGAGATGAGACGTAAGAAGATTCAGTCGACTCCATTGTTGTTGTTGTTGTTGTTCGTCCTCTTCCTCTTGAGCAAAAGAACCTAATTCTCACTCCTTCGTTTCAAAGAGCGAGAGAAAGAGACGGTTAATTTCAGAGAGAAAGGTGAGAGAAAAATTTCATAGTAATGGCGTGAGGTGAGAAATGAAAGTGTTGAGAGAATTGAAAAAAGTGAAAACGGCGGAGAGGGAAGAGAGAGAGGGGGTTGAACCGGAGATGAGAGAGAAAGAGAGAGAGAGAGGAATGAGAAGAAGAGAAGAAGTTGCTGCTCTGAAGTTTTGGTTTGTTTCTGCTCTCTACAACGTCC
TARGET=550,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8487
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=0.830836
PRIMER_LEFT_SEQUENCE=TTCATAGTAATGGCGTGAGGTG
PRIMER_RIGHT_SEQUENCE=ACCAAAACTTCAGAGCAGCAAC
PRIMER_LEFT=440,22
PRIMER_RIGHT=604,22
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=60.831
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=0.9682
PRIMER_LEFT_1_PENALTY=0.017846
PRIMER_RIGHT_1_PENALTY=0.950358
PRIMER_LEFT_1_SEQUENCE=TTCATAGTAATGGCGTGAGGTG
PRIMER_RIGHT_1_SEQUENCE=CCAAAACTTCAGAGCAGCAACT
PRIMER_LEFT_1=440,22
PRIMER_RIGHT_1=603,22
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=60.950
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.9819
PRIMER_LEFT_2_PENALTY=0.151106
PRIMER_RIGHT_2_PENALTY=0.830836
PRIMER_LEFT_2_SEQUENCE=GAGCGAGAGAAAGAGACGGTTA
PRIMER_RIGHT_2_SEQUENCE=ACCAAAACTTCAGAGCAGCAAC
PRIMER_LEFT_2=390,22
PRIMER_RIGHT_2=604,22
PRIMER_LEFT_2_TM=60.151
PRIMER_RIGHT_2_TM=60.831
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=0.9840
PRIMER_LEFT_3_PENALTY=0.153161
PRIMER_RIGHT_3_PENALTY=0.830836
PRIMER_LEFT_3_SEQUENCE=GTTGTTGTTCGTCCTCTTCCTC
PRIMER_RIGHT_3_SEQUENCE=ACCAAAACTTCAGAGCAGCAAC
PRIMER_LEFT_3=329,22
PRIMER_RIGHT_3=604,22
PRIMER_LEFT_3_TM=60.153
PRIMER_RIGHT_3_TM=60.831
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=1.0568
PRIMER_LEFT_4_PENALTY=0.225996
PRIMER_RIGHT_4_PENALTY=0.830836
PRIMER_LEFT_4_SEQUENCE=CGTGAGGTGAGAAATGAAAGTG
PRIMER_RIGHT_4_SEQUENCE=ACCAAAACTTCAGAGCAGCAAC
PRIMER_LEFT_4=453,22
PRIMER_RIGHT_4=604,22
PRIMER_LEFT_4_TM=59.774
PRIMER_RIGHT_4_TM=60.831
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_00664 Ps_contig_mira-and-tgicl-ass_franssen_6431
SEQUENCE=CCGTTTCACTTTTTCTAATTCTCTCAACACTTTCATTTCTCACCTCACGCCATTACTATGAAATTTTCTCTCACCTTCTCTCTGAAATTAAACCGTCTCTTTCTCTCGCTCTTTGGAACGAAGGAGTGAGAATTAGGTTCTTTTGCTCAAGAGGAAGAGGACGAACAACAACAACAACAATGGAGTCGACTGAATCTTCTTACGTCTCATCTCCAGAAGGACCGAGGAAGCACGCTTACTTAAAACACCTCCTCCGGAATCACCTTCTCAAGATT
TARGET=164,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0080
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.005674
PRIMER_LEFT_SEQUENCE=TTTTGCTCAAGAGGAAGAGGAC
PRIMER_RIGHT_SEQUENCE=TCCGGAGGAGGTGTTTTAAGTA
PRIMER_LEFT=140,22
PRIMER_RIGHT=257,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=118
PRIMER_PAIR_PENALTY_1=0.0269
PRIMER_LEFT_1_PENALTY=0.021195
PRIMER_RIGHT_1_PENALTY=0.005674
PRIMER_LEFT_1_SEQUENCE=TTAAACCGTCTCTTTCTCTCGC
PRIMER_RIGHT_1_SEQUENCE=TCCGGAGGAGGTGTTTTAAGTA
PRIMER_LEFT_1=87,22
PRIMER_RIGHT_1=257,22
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=171
PRIMER_PAIR_PENALTY_2=0.1152
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.005674
PRIMER_LEFT_2_SEQUENCE=TTTGGAACGAAGGAGTGAGAAT
PRIMER_RIGHT_2_SEQUENCE=TCCGGAGGAGGTGTTTTAAGTA
PRIMER_LEFT_2=111,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=0.1290
PRIMER_LEFT_3_PENALTY=0.123278
PRIMER_RIGHT_3_PENALTY=0.005674
PRIMER_LEFT_3_SEQUENCE=CTTTTGCTCAAGAGGAAGAGGA
PRIMER_RIGHT_3_SEQUENCE=TCCGGAGGAGGTGTTTTAAGTA
PRIMER_LEFT_3=139,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=60.123
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=0.1360
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.005674
PRIMER_LEFT_4_SEQUENCE=TCTTTCTCTCGCTCTTTGGAAC
PRIMER_RIGHT_4_SEQUENCE=TCCGGAGGAGGTGTTTTAAGTA
PRIMER_LEFT_4=97,22
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_00665 Ps_contig_mira-and-tgicl-ass_franssen_6437
SEQUENCE=GGCGGGGATCACAAACAGAATACACCTCAATTCTTCTCTCTCATAACAATCTCTCGTCTTCTCATAAATTAAAAACCCTTTAAACTTTAATTTAATTTTTTTGTTTTAGTAAAAATAACTTATTATTATTATTATTATCATCATGGCTCAAAGAAACAGAAAATGAAGAAACTGAATTCAAAGTACCTGAAACGATATCGCTTTGCATCAACAACTGTGGCGTAATCGGTAATCCTTCAACGAACAATATGTGTCAAAATTGCTTCACCGCTTCAACCGTAACCACTTCTTCCACCGTCGGGATCGGAATAA
TARGET=120,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8279
PRIMER_LEFT_PENALTY=1.697705
PRIMER_RIGHT_PENALTY=0.130244
PRIMER_LEFT_SEQUENCE=CGGGGATCACAAACAGAATAC
PRIMER_RIGHT_SEQUENCE=GTTGAAGGATTACCGATTACGC
PRIMER_LEFT=2,21
PRIMER_RIGHT=240,22
PRIMER_LEFT_TM=59.302
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=1.8373
PRIMER_LEFT_1_PENALTY=1.707042
PRIMER_RIGHT_1_PENALTY=0.130244
PRIMER_LEFT_1_SEQUENCE=GGGATCACAAACAGAATACACC
PRIMER_RIGHT_1_SEQUENCE=GTTGAAGGATTACCGATTACGC
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=240,22
PRIMER_LEFT_1_TM=58.293
PRIMER_RIGHT_1_TM=59.870
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=1.8373
PRIMER_LEFT_2_PENALTY=1.707042
PRIMER_RIGHT_2_PENALTY=0.130244
PRIMER_LEFT_2_SEQUENCE=GGGGATCACAAACAGAATACAC
PRIMER_RIGHT_2_SEQUENCE=GTTGAAGGATTACCGATTACGC
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=240,22
PRIMER_LEFT_2_TM=58.293
PRIMER_RIGHT_2_TM=59.870
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=1.8579
PRIMER_LEFT_3_PENALTY=1.727609
PRIMER_RIGHT_3_PENALTY=0.130244
PRIMER_LEFT_3_SEQUENCE=GGGATCACAAACAGAATACACCT
PRIMER_RIGHT_3_SEQUENCE=GTTGAAGGATTACCGATTACGC
PRIMER_LEFT_3=4,23
PRIMER_RIGHT_3=240,22
PRIMER_LEFT_3_TM=59.272
PRIMER_RIGHT_3_TM=59.870
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=1.9344
PRIMER_LEFT_4_PENALTY=1.697705
PRIMER_RIGHT_4_PENALTY=0.236697
PRIMER_LEFT_4_SEQUENCE=CGGGGATCACAAACAGAATAC
PRIMER_RIGHT_4_SEQUENCE=ATTGTTCGTTGAAGGATTACCG
PRIMER_LEFT_4=2,21
PRIMER_RIGHT_4=247,22
PRIMER_LEFT_4_TM=59.302
PRIMER_RIGHT_4_TM=60.237
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_00666 Ps_contig_mira-and-tgicl-ass_franssen_6449
SEQUENCE=TTCGGGGTAAGCASWCRKKSATTTCATGSATCATCATCACTCACATATCATCCAATCCAATCCAATATTCCCAAAATGCTCAATGCTCTTTCTTCTTCGTTCTCAATTGTTCCCTTTCATGTCAATTATTCACTCTTTACTCAAACTCAGTTATTAGTTCCCACTAATCTTAATTACCCTTTTCTTAATACAGCTACCAGAAAGTTCAGGACTTTTCAGCTCAAGGCTGGATTTTGGGAGTCAATTAAATCTGGGTTGACGAAGAATAACACCGCCACGCAGGTTATCGATCCAGAGTTAACAGATGAAGAAGACGAAGAACCTCTACCTCAAGAGTTTGTGCTTGTTGAAAAGACTGAACCGGATGGAACAATTGAACAGATAATGTTCTCTTCGGGTGGAGATATTGATGTTTATGATCTTCAAGCCCTATGTGACAAGGTAGGGTGGCCTCGTAGGCCGTTATCAAAATTAGCTGCTGCTTTGAAAAATAGCTATATGGTGGCCTGTCTCTATTCCATAAGAAAGTCACCAGGGTCAGAGGGGAATGAACAAAAGATATTAATTGGCATGGCTCGTGCTACGTCAGACCATGCCTTCAATGCCACAATTTGGGATGTCCTAGTTGATCCCGGCTACCAGGGCCAAGGTCTTGGCAAAGCTCTCGTAGAGAAACTGATTCGAGCACTTTTGCAAAGGGACATCGGTAATATAACATTGTTTGCAGATAGTAAAGTTGTGGAATTTTATCGCAATTTAGGTTTCGAAGCTGACCCAGAGGGCATAAAAGGCATGTTTTGGTACCCAACTAACTAATTTAAAAGGAAGTTACCTTTATGCTATTGTTACATAGTTTAACATTGCTACCTGAAGTATACAACAATGCCATGTTTGTAATATGAAAGTATGAAGATTGGATATCAATCCTGTAATTATTCTGTAAGGAAGTGGTAGCAGGATATAAAATAGCCATCTTTGATAAAA
TARGET=50,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00667 Ps_contig_mira-and-tgicl-ass_franssen_6454
SEQUENCE=CGACNCACAATAATATTCTTCTCTCTACCKACCCTTTTCCTTTCCTTCTCTCTCTCTCTCTTCAGCTTCTACATTCCTTCCTCTTTAAAGCAAATTCTAGAGAGAGAGAAAGCGAGAGAGTGTGATTTTCGAGCTTTTCACAATGGCAGCTTCTATGAAATGGTTTCTCAATTTGTTGTTACTTTTTGTGCTGCTCGTTGAAGAAGAAGCTATGGCTTCTACACAGATCTCTCAGTTCAGCAATGTGGATACTGAGACACACAGGTAACAAGTA
TARGET=47,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00668 Ps_contig_mira-and-tgicl-ass_franssen_6454
SEQUENCE=CGACNCACAATAATATTCTTCTCTCTACCKACCCTTTTCCTTTCCTTCTCTCTCTCTCTCTTCAGCTTCTACATTCCTTCCTCTTTAAAGCAAATTCTAGAGAGAGAGAAAGCGAGAGAGTGTGATTTTCGAGCTTTTCACAATGGCAGCTTCTATGAAATGGTTTCTCAATTTGTTGTTACTTTTTGTGCTGCTCGTTGAAGAAGAAGCTATGGCTTCTACACAGATCTCTCAGTTCAGCAATGTGGATACTGAGACACACAGGTAACAAGTA
TARGET=99,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00669 Ps_contig_mira-and-tgicl-ass_franssen_6464
SEQUENCE=ATGGTTTATATTGTGTGAAAACTAAACATGTAATTAGGATGTTTTTGTTATTGTKTTTTGTCATGTAGAGTTATTATTATAGCTACATATAGGCTTTAATGGCAGAGTTATGTGCAGTGCAGTGCAGTTTACAAAGACTTGTGTTAATGGGACTGTGAGAGAATTGATTATAGATATCTATGAGTATTGGAAGTAAAAGACTATTTTTCTTCCTCCCTTTTTGAAAACCAAAAATTTAATGCAATAAAACATTGAT
TARGET=112,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00670 Ps_contig_mira-and-tgicl-ass_franssen_6491
SEQUENCE=CGTACAGAAGGCTCTGTGATTCCTTCAGTAGAAGATATCTCATCCTTTGGTTGAGCCGTAATTCTCTTCACTTCTGAAGCATGAAACGGATGAACCTGACGAATTGGTTTGGGGCATGAAACTTGAAGGCGTAGAGGACGATGAAAATGATCAACTGAGGATAAAGAAGAGATAGGATGGTGAAGGAAGTTGAGGGAAGATGAAACCCTGATTCTGAGAACGCCATTGTGATTCAATGGAATTTGAACCACAGAAGCCAAACCCATAATTCTTGAGTTCAGAAAGAAGAATTAAAACCGAATGAATCAACCTCTTCTTTTCTCCTTCTCAGACAGTTTGTTTGTTTGCGCTACAAGTACAGAAGATCGTTGTTGTTGTTTTTGGGTTTTGGGTTAAGGAGTGTGGTCACAAGCACACTCRGAATATTATTTACATATTTTATTCTMSYCCCAT
TARGET=335,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00671 Ps_contig_mira-and-tgicl-ass_franssen_6491
SEQUENCE=CGTACAGAAGGCTCTGTGATTCCTTCAGTAGAAGATATCTCATCCTTTGGTTGAGCCGTAATTCTCTTCACTTCTGAAGCATGAAACGGATGAACCTGACGAATTGGTTTGGGGCATGAAACTTGAAGGCGTAGAGGACGATGAAAATGATCAACTGAGGATAAAGAAGAGATAGGATGGTGAAGGAAGTTGAGGGAAGATGAAACCCTGATTCTGAGAACGCCATTGTGATTCAATGGAATTTGAACCACAGAAGCCAAACCCATAATTCTTGAGTTCAGAAAGAAGAATTAAAACCGAATGAATCAACCTCTTCTTTTCTCCTTCTCAGACAGTTTGTTTGTTTGCGCTACAAGTACAGAAGATCGTTGTTGTTGTTTTTGGGTTTTGGGTTAAGGAGTGTGGTCACAAGCACACTCRGAATATTATTTACATATTTTATTCTMSYCCCAT
TARGET=368,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00672 Ps_contig_mira-and-tgicl-ass_franssen_6499
SEQUENCE=TGTCATGATTTAGAAAGATTTTCTGTCATCAATCGATTTGCCAAATTTCATGGACGGGGGCAGAATGATGGGGCAGAGACTTCACAATCCTCAGCAGCAACGGCGAATGCTCCGAAATCATTTGTCCAGAGATATGTTACTGGATATTCAATGCCGGAAACTTTCCCAGACAGGGTACAATGTCTTTCACTTTGATAATTAATTAATTCATTTGTCCATCTTTCTTGGTCGGTTTTCTACGCAATCTCCTGTTTTTGGTCCTTTTCTTTCAAGTTATATTATTGGACATCATCGCCATAGAACCATACAACTGGCAATGTAGGAGAAGAATGGTACTAGGTTGCCTCCTCAACCAATGGAGGCTGTTAGATCTTTCCCATCTATATTTTGTATGTATGTATGTCTAGATCTTCGCAATCTAGTTATCTTTTAACTTTTTGGTTTTCAGGTTC
TARGET=196,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1172
PRIMER_LEFT_PENALTY=0.099308
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=AAACTTTCCCAGACAGGGTACA
PRIMER_RIGHT_SEQUENCE=CAGTTGTATGGTTCTATGGCGA
PRIMER_LEFT=157,22
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=0.1172
PRIMER_LEFT_1_PENALTY=0.099308
PRIMER_RIGHT_1_PENALTY=0.017846
PRIMER_LEFT_1_SEQUENCE=AACTTTCCCAGACAGGGTACAA
PRIMER_RIGHT_1_SEQUENCE=CAGTTGTATGGTTCTATGGCGA
PRIMER_LEFT_1=158,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=155
PRIMER_PAIR_PENALTY_2=0.1645
PRIMER_LEFT_2_PENALTY=0.146683
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=TGTTACTGGATATTCAATGCCG
PRIMER_RIGHT_2_SEQUENCE=CAGTTGTATGGTTCTATGGCGA
PRIMER_LEFT_2=134,22
PRIMER_RIGHT_2=312,22
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.7000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.1655
PRIMER_LEFT_3_PENALTY=0.146683
PRIMER_RIGHT_3_PENALTY=0.018835
PRIMER_LEFT_3_SEQUENCE=TGTTACTGGATATTCAATGCCG
PRIMER_RIGHT_3_SEQUENCE=TGAGGAGGCAACCTAGTACCAT
PRIMER_LEFT_3=134,22
PRIMER_RIGHT_3=350,22
PRIMER_LEFT_3_TM=59.853
PRIMER_RIGHT_3_TM=60.019
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=0.2166
PRIMER_LEFT_4_PENALTY=0.099308
PRIMER_RIGHT_4_PENALTY=0.117281
PRIMER_LEFT_4_SEQUENCE=AAACTTTCCCAGACAGGGTACA
PRIMER_RIGHT_4_SEQUENCE=TGCCAGTTGTATGGTTCTATGG
PRIMER_LEFT_4=157,22
PRIMER_RIGHT_4=315,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=59.883
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_00673 Ps_contig_mira-and-tgicl-ass_franssen_6499
SEQUENCE=TGTCATGATTTAGAAAGATTTTCTGTCATCAATCGATTTGCCAAATTTCATGGACGGGGGCAGAATGATGGGGCAGAGACTTCACAATCCTCAGCAGCAACGGCGAATGCTCCGAAATCATTTGTCCAGAGATATGTTACTGGATATTCAATGCCGGAAACTTTCCCAGACAGGGTACAATGTCTTTCACTTTGATAATTAATTAATTCATTTGTCCATCTTTCTTGGTCGGTTTTCTACGCAATCTCCTGTTTTTGGTCCTTTTCTTTCAAGTTATATTATTGGACATCATCGCCATAGAACCATACAACTGGCAATGTAGGAGAAGAATGGTACTAGGTTGCCTCCTCAACCAATGGAGGCTGTTAGATCTTTCCCATCTATATTTTGTATGTATGTATGTCTAGATCTTCGCAATCTAGTTATCTTTTAACTTTTTGGTTTTCAGGTTC
TARGET=389,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00674 Ps_contig_mira-and-tgicl-ass_franssen_6500
SEQUENCE=CTCCTACATTGCCAGTTGTATGGTTCTATGGCGACGAAGTGCAATAATATAATTTGAAAGAAATAGACCAGAAATAGGAGATTGGGCAGACAACTAATCAAGAAAGATGGAATAATGAATTAATTAATTAATTATCAAAGTGAAAGACATTGTACCCTGTCTGGGAGATTCCTAGGCAATGGAACTTCAGTAACAAGTTTCACGGGAAAAAATCTCTGAAAAGTAGTATCAGAGGATGATGAGGTCTCTGCGTTGATACCACTGTCTTKATGAACTGCAATCCCGCGTACT
TARGET=118,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00675 Ps_contig_mira-and-tgicl-ass_franssen_6502
SEQUENCE=TTTTCTTTTCCTCTGTCATTCCTCCTTTATAAGAGAACTTCTGACGATTCAACCAAGACTTGAAGATAAAAGTAATAACAAATCCCCAAATTTCCAATGTTCTCTGTATAGTCGAGTAAGTCTTGAACCTACTCCAACGACCACCGGGCGCAACCGCCACCTCAACTTGTGCTTCACCATTTCGTTTAAACCACGCATCTTCTTTTCCAATCTCCTCCAACCTCTTCTTCCTCCCAACTTCCTTCTGCTTCGAGGTTTCAACAACTTCCGCCGCCACGCCATTTCCATTTACATACTTCACCAACCTTCCATTTTCACTACCGTTAACGTAACCTCTAACGGAACCGTTTTTACCGTTTACAACACTTGTAGCAGCTCCGTTTCTACTCCACTTCACATCGCTTGCTCTTTCTTCAATCAATGCACTTTCTTCCTTGACAGCGCGAATTCGAGTGCGAAGAGTAACATTGTATTTGTAGTTTGAAACGGAATTTAAAGAGCGGAATGTAGTGAGAGAACGACGACGTTTTGGTCTGATTTGTGGAGCAAGAAAATTGAGTTCGGTTAAGAGTAGAGAAGATGAAGCCATTGATGAGAGAGAGAGAGAGGGGATCAAACTGGAACCCTAGTTTTCATGGGGGTTCGATTGGAAGGAAAGTGTGTGAAAGAGTGGAAGAAAAGAAAGAAGAGTAGTWCCCCGAC
TARGET=594,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00676 Ps_contig_mira-and-tgicl-ass_franssen_6510
SEQUENCE=CACCACTAGGAACATTTATATTGTATGATATACAGATATTCAAGTTCTATCTATTTACATAATCTTTAATCTACCTAGAAATGTAATCCAAGTTACATGAATAATCTAACATAGTTGCRAGAACATATACAATGATGAATTTATCCTAAAAACAATTGGAATAAATAATCAGGTGAAGCAATCTGCAAATGGATAGAAAGAAAGAAGTGATGACTGCTAGCAATTCTCGGCTCTGGAATACTTTGTGGGAAGAGATGAACACCTTTCATTCATGTGAGGATAAGGTTGCATGGTATTGTATCCAGGTAACTCCATCCGATACTTGCCTTTGATCTGACAATATGGAAGCTTCACGGTGTCCCCGTCGTTGCACCATGTTGGCAACCGGCTAGAATTGTTCTCGAAAAAATTGAGAATGTAAGCATCTTTTATCGTAAACTCTGTCACTTGGATAGAACTAGCAATTGGATCAAACAGTCCTGCCTCCTTGTACATTGCAAGTATAAAAGCAACACAAGAAGTTGACTTTCCATCACTGTAGGTCCAGTCATCCTGTTCTGGAATCGTCAGCAGTTCATCAAAAGATGATCCACGCCTTTCAACTTCTACTAATACTTCTGAAAGGTCGAGCCCTTTAGTTCCAAGTCGTTTGTTCAAGGCTTCATTCCACATATTTCCAGCATATTCAGGTTTAAGTTGACTCCAAATTGTCATAACACAAGCAACCACATTAGCATCCAAGGGAGGAGGATAGTTCGCAGTTAAAGTATCTATCCAACTGAAAATCATGTTATGGTARCCATAAGGTTTTCCGTCCATACTCAAAGCATACTCCCACGCTGCAGTCTCATTAAACCTCG
TARGET=195,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00677 Ps_contig_mira-and-tgicl-ass_franssen_6532
SEQUENCE=ATCAAAATCAATACTTTCTTAATGTTACTTTTCTTTAATTACATCCACTAACTAATAGTATTATATACATTACTTCTTTAACATTATAACGTTATACTGTTAATACGAAAAAAGTAACACCAATAAATGGTCACACAGCTAGCAATATATTGTTCCCCTTTTTAGGTAAATTAAATGAAGTACAAGTTATTTATGCAGTGGCAAACAAAAGTCTTACACTTAATGGTCATCAACTTACTAAAATCAATACCAATAACTGGTGTATTAGCATACTTTCTTTTTGATGATGCACCTTTAGACACAGTCGATCACTAGATAACGCCTAGTTAAGTCTTTCTCAGGATAACATAACTGGCTCCATTTGTAGATTTCTGTATCTTGGAGATTCTCTTCAGAACATCGGCAAACGCTTTCTTATCCTCTGAACATCTTAATCTGGCCTTAAATTTACCCACAAGCTCCTGTGTGGTTAATGGGGCCCTTTGCATTAGAACAGCTGTAATTTCTTCCGCTGTTACAGGTCCAGAAGCTGCATTAGAAGACCCAGTGTTTGATGAAGGTGCTGGTGTACCTCTTGGAGGTGCGCTAGTTTTAGCTGTAGATCCATTTACATCTTTTTCAGATGGTTTTGTTTCATTTTCTGTCTTTACTTTCTTATTAGGTGGTACACTATTAGATGGCTTTGCTTCTTCATTTGCTTTTCTTTTCCCCCCCTTTGAAGACTTTGAAGTAGGTGTTCCACGAGCAGATCCTGTTGCTGTAGGTTTCGAAGGACTGTTGTCAACAGGTTCTTCTTTAGGTTCCTTTTGCTTTGTAGCAGTAGCAGGAGGAACACCACCCTCTTCATCAATATCGTCGTCGTCATCGTCATCATCGTCTGCTTCAGAATCACTCAGACCACCACTTCGCCCAAGTAACTTCTTCAGCTCTTTCCCAGATTTACTCAGACCACCTCCTTCTTCATTGTCTTCATCCTCATCCTCTTCATCCTGCTTGATTTCAGGAGGAGCAGGAACTTCAGGAGCCAAATCCTCCCTTTCCTCGGGATCATTACCAACAGCTTCATCATCGTCTGTGAAAATCTCTTCATGCTCCCAATCATCACCCTTTTCAACATCATAATCATCCTCATCATGGTCCCCTCCCCTTGGACCTTCATCTTCATCTTCATCATAGCCAGGTTTTTTATTCAGCCCAAGTCTATTCTTCCTATCTACCTCCTCATCTTCATCTTCCTCTCCTTTATCAGAGGAAGGACCTTCATCATCATCATCACCAGTCTTTTTTTYSGCCTCTCCCACCACCTGCATTGTCTTTTCCTTTTCATCTTAATTTTGCATTGTTCCAACCGAATGCTAAGG
TARGET=1261,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00678 Ps_contig_mira-and-tgicl-ass_franssen_6544
SEQUENCE=TATGGATCTAAATCCCTTTTTAAAATTTTACTTTCATGGCATAAGCAAGACAAAAAAATAAATTTTAATAATAATAATAAACATAAAAAAATTGACAATATCCATAGTTTCTATCTCCTACACATGAAATTAAACATAAAATAACAGAAAAAGTTTGAACTAGTAGTAAGTAGTAACTAGTACATAGACAGATCTTAGATCTAGACGATCTCGATGTTTCAGATTCGAATTCCGCCGCCATATCCTTGAAATCTTAGGAGTTCTCCACACCCCTTAATTAATCTTTATAAAAGACAATGAGACTTGATGTCTCCTTGAAGAACTGTAGTTTTCAGGTAACCCTCATTTCATGTATAGTATAGGTAGTATGGGAGAAGGCTATCCCTTGTTTCCTGTATATGGAACAAAGAACAGTTTTGAGATGTGTCCCC
TARGET=66,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8825
PRIMER_LEFT_PENALTY=1.596540
PRIMER_RIGHT_PENALTY=0.285981
PRIMER_LEFT_SEQUENCE=ACTTTCATGGCATAAGCAAGAC
PRIMER_RIGHT_SEQUENCE=TAAGATTTCAAGGATATGGCGG
PRIMER_LEFT=29,22
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=58.403
PRIMER_RIGHT_TM=60.286
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=2.3985
PRIMER_LEFT_1_PENALTY=1.596540
PRIMER_RIGHT_1_PENALTY=0.801964
PRIMER_LEFT_1_SEQUENCE=ACTTTCATGGCATAAGCAAGAC
PRIMER_RIGHT_1_SEQUENCE=CGGAATTCGAATCTGAAACATC
PRIMER_LEFT_1=29,22
PRIMER_RIGHT_1=233,22
PRIMER_LEFT_1_TM=58.403
PRIMER_RIGHT_1_TM=60.802
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=2.3985
PRIMER_LEFT_2_PENALTY=1.596540
PRIMER_RIGHT_2_PENALTY=0.801964
PRIMER_LEFT_2_SEQUENCE=ACTTTCATGGCATAAGCAAGAC
PRIMER_RIGHT_2_SEQUENCE=GGAATTCGAATCTGAAACATCG
PRIMER_LEFT_2=29,22
PRIMER_RIGHT_2=232,22
PRIMER_LEFT_2_TM=58.403
PRIMER_RIGHT_2_TM=60.802
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=204
PRIMER_PAIR_PENALTY_3=2.4794
PRIMER_LEFT_3_PENALTY=1.596540
PRIMER_RIGHT_3_PENALTY=0.882866
PRIMER_LEFT_3_SEQUENCE=ACTTTCATGGCATAAGCAAGAC
PRIMER_RIGHT_3_SEQUENCE=AGGGGTGTGGAGAACTCCTAAG
PRIMER_LEFT_3=29,22
PRIMER_RIGHT_3=273,22
PRIMER_LEFT_3_TM=58.403
PRIMER_RIGHT_3_TM=60.883
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=2.6165
PRIMER_LEFT_4_PENALTY=1.596540
PRIMER_RIGHT_4_PENALTY=1.019966
PRIMER_LEFT_4_SEQUENCE=ACTTTCATGGCATAAGCAAGAC
PRIMER_RIGHT_4_SEQUENCE=GGGGTGTGGAGAACTCCTAAG
PRIMER_LEFT_4=29,22
PRIMER_RIGHT_4=272,21
PRIMER_LEFT_4_TM=58.403
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=244
=
PRIMER_SEQUENCE_ID=SSR_00679 Ps_contig_mira-and-tgicl-ass_franssen_6546
SEQUENCE=TTWTTTCTACTATGATATTTGTCCATTTTCTTTCACTATCATTCTCAATAAAAATAAAGAAACCCTACACACTTGCTTCCTGTCTCATAATGCCAACTTGATTTGAAGAACATCTGTCATATGGATCTAATCCTTTTAAATTTCTTCATGGCATAAGCAAGACAAAAAAATAAATAATAATAATAATAAACATAAAAAAATTGACAATATCCATAGTTTCTATCTCCTACACATGAAATTAAACATAAAATAACAGAAAAAGTTTGAACTAGTAGTAAGTAGTAACTAGTACATAGACAGATCTTAGATCTAGACGATCTCGATGTTTCAGATTCGAATTCCGCCGCCATATCCTTGAAATCTTAGGAGTTCTCCACACCCTAAATTGTTTTTATCAAAAAAGAAGAAGAAATTAAACATAAAATTATAACTTTTTTTCCTGTCAGTAAAATGGTTTAATTTGTTGAAGAATTTTGTTCTTTAAGAGTGAAAACTCAAAGCCCTAAAGATAAGGTTTTTTCATAACCGACAAATGATCCAACTGGTTACTCTCTTATCTCTAGATGGGTGGTGTACATGCACTTCCAAACAATAAAATAAACTAATAAAACAAACCATGTTTCTTGGCAGTATGAATCAAAGACAAACTATAGGTTTTTCTAGATAATTTAAATTAATTAGTAGTACTAAAACTAGTACTAGTAGGTTGACTGAGTATATAAAATCAAAATTCATTGACTAAATATAAATTAATATGATTTTGATGAAGTAACTGTTGGATAGCATGCTAAGAAACAACCAAGAAGAATCCATTCAAGAACAATCTAAAAACAACTCTCTTCGAAATTTTGAATGAAATTTGATGTATTATTGGTATATACCTGGAAAATTTATGAATGAGATTTGATGTATTATTGGTTTTCTGTACTGAATCCCCAATTTGAAAGCTAGGGTTAGTGTTTTCATTAAAAAGGAAAAACAAAAAGACTGAATCGAAACGACAAAGACTGAATTCATGTAAACCAGATCTTGACATCAAGACGACTATGTTAAAAACTGATAATAGAAGAAGATGAGAAGGAAAGAGAGATGGTGGTGGGGTGACAGATAGAGGGTGAGCACGCATAGCTTCAAGCATGAACCATCATCATGCAAGAAAACCACCATGTGTGCTCACTCTCTTCTGTCACCGCCCTTACCCTTAATTAATCTTTATAAAAGACAATGAGACTTGATGTCTCCTTGAAGAACTGTAGTTTTCAGGTAACCCTCATTTCATGTATAGTATAGGTAGTATGGGAGAAGGCTATCCCTTGTTTCCTGTATATGGAACAAAGAACAGTTTTGAGATGTGTCCCCCGACT
TARGET=173,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00680 Ps_contig_mira-and-tgicl-ass_franssen_6547
SEQUENCE=AATTTTTTAGTAAAATCATCATTTATTAATTTTTTTCGAAGATCCAGAATAAAAACACTTCAATTCAAAACTAATAATCTTACATGCATTAAATTAATTTGATGAAAGTAAAAAATGAAAGCAACAGTATAACAAGAGAGAAACAAAATTGACAATACACATCAAAATCACTATGATATTTGTCCATTTTCTTTCACTATCATTCTCAATAAAAATAAAGAAACMCTACACACTTGCTTCCTGTCTCATAATGCCAACTTGATTTGAAGAACATCTGTCATATGGATCTAATCCTTTTAAATTTCTTCATGGCATAAGCAAGAACAAAAAAATAAATAATAATAATAAACATAAAAAATTGACAATATCCATAGTTTCTATCTCCTACA
TARGET=335,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00681 Ps_contig_mira-and-tgicl-ass_franssen_6548
SEQUENCE=CGTTATGTTTCTGAGGTAAAGAGAAATTGARAAGAAATAGGCCATGATATCAAAATAACTTCCAAGAATTATCCTTAGTACCTCATTATTCCTCTTCAAACGACGAAATCAGCACTTCCTTCCAGCATCATCACATTCTGAAAACTTAGTTTGATAATCAGAAGAATTTTTGCATCATCATCTTGTTCCTCTTGCATGCATCATCGAAGTCAGAGAGACACAGTGAACCGCTTATTCTGAAAAGTACTGCTTCTCTCTCTCACTCTTTTTCTGTCTTTCTCCTCCTCGTA
TARGET=252,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00682 Ps_contig_mira-and-tgicl-ass_franssen_6552
SEQUENCE=CTTGAGTCTTGTTTTCTTTTAGTTTGCGCATGTCCAAGATTAGTTAACTTCATTTTGATCTTAGGATTCTGCATTGCGATGCGTGTTCTTTCCTTGATCTTGTGCAAAGTTTCGGGAGAATGTTTTCTTCCTTTGTTCCATGGAGTGTTTCCTTTATTAGCTTTAGAAATCCTCGATCTTCTCAACTTCTCCCTCTCGTCGACTGGTTTTTCAGATAAATCGAGCATAATTCCGGGTTGGTGATTGTGCGGTTGAACAATGGTGGTGGCGGCGGCGGCGACGGCTTTGATGAGGAAGGGTAACCTAGGGCGTGGAACGTTGAACTTGTGTTTTCGAAATAAATTCCAATTCCAGCCGGAAATTTGAGAGTGTTTGACATGATAGTAATATGGCAATGGAGCCGTCAGTAAATGTGCATTTCTTATCTCAATCAAAGGCATGGGGAGAATGAAATTAGAAAAAGGAAGCAGAAAGCAGCAGCAGCATCTGAGAATATATCATATGTATGTGTGTTTGCGCGTTTGGTAATGGTGGGATATAGGAAGGAAACCACAGAGAAGAGAAAGAGGATAACGTTGTGAATGCTCCCCCGA
TARGET=267,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2010
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.195462
PRIMER_LEFT_SEQUENCE=TTCTCAACTTCTCCCTCTCGTC
PRIMER_RIGHT_SEQUENCE=CCCTAGGTTACCCTTCCTCATC
PRIMER_LEFT=178,22
PRIMER_RIGHT=308,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.195
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=131
PRIMER_PAIR_PENALTY_1=0.2758
PRIMER_LEFT_1_PENALTY=0.080323
PRIMER_RIGHT_1_PENALTY=0.195462
PRIMER_LEFT_1_SEQUENCE=CTTGATCTTGTGCAAAGTTTCG
PRIMER_RIGHT_1_SEQUENCE=CCCTAGGTTACCCTTCCTCATC
PRIMER_LEFT_1=92,22
PRIMER_RIGHT_1=308,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=60.195
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.2854
PRIMER_LEFT_2_PENALTY=0.005574
PRIMER_RIGHT_2_PENALTY=0.279852
PRIMER_LEFT_2_SEQUENCE=TTCTCAACTTCTCCCTCTCGTC
PRIMER_RIGHT_2_SEQUENCE=TCTAATTTCATTCTCCCCATGC
PRIMER_LEFT_2=178,22
PRIMER_RIGHT_2=457,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.280
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.3198
PRIMER_LEFT_3_PENALTY=0.124370
PRIMER_RIGHT_3_PENALTY=0.195462
PRIMER_LEFT_3_SEQUENCE=TCTTCTCAACTTCTCCCTCTCG
PRIMER_RIGHT_3_SEQUENCE=CCCTAGGTTACCCTTCCTCATC
PRIMER_LEFT_3=176,22
PRIMER_RIGHT_3=308,22
PRIMER_LEFT_3_TM=60.124
PRIMER_RIGHT_3_TM=60.195
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=0.3330
PRIMER_LEFT_4_PENALTY=0.137489
PRIMER_RIGHT_4_PENALTY=0.195462
PRIMER_LEFT_4_SEQUENCE=ATGCGTGTTCTTTCCTTGATCT
PRIMER_RIGHT_4_SEQUENCE=CCCTAGGTTACCCTTCCTCATC
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=308,22
PRIMER_LEFT_4_TM=60.137
PRIMER_RIGHT_4_TM=60.195
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_00683 Ps_contig_mira-and-tgicl-ass_franssen_6552
SEQUENCE=CTTGAGTCTTGTTTTCTTTTAGTTTGCGCATGTCCAAGATTAGTTAACTTCATTTTGATCTTAGGATTCTGCATTGCGATGCGTGTTCTTTCCTTGATCTTGTGCAAAGTTTCGGGAGAATGTTTTCTTCCTTTGTTCCATGGAGTGTTTCCTTTATTAGCTTTAGAAATCCTCGATCTTCTCAACTTCTCCCTCTCGTCGACTGGTTTTTCAGATAAATCGAGCATAATTCCGGGTTGGTGATTGTGCGGTTGAACAATGGTGGTGGCGGCGGCGGCGACGGCTTTGATGAGGAAGGGTAACCTAGGGCGTGGAACGTTGAACTTGTGTTTTCGAAATAAATTCCAATTCCAGCCGGAAATTTGAGAGTGTTTGACATGATAGTAATATGGCAATGGAGCCGTCAGTAAATGTGCATTTCTTATCTCAATCAAAGGCATGGGGAGAATGAAATTAGAAAAAGGAAGCAGAAAGCAGCAGCAGCATCTGAGAATATATCATATGTATGTGTGTTTGCGCGTTTGGTAATGGTGGGATATAGGAAGGAAACCACAGAGAAGAGAAAGAGGATAACGTTGTGAATGCTCCCCCGA
TARGET=473,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3624
PRIMER_LEFT_PENALTY=0.279852
PRIMER_RIGHT_PENALTY=0.082508
PRIMER_LEFT_SEQUENCE=GCATGGGGAGAATGAAATTAGA
PRIMER_RIGHT_SEQUENCE=GGTTTCCTTCCTATATCCCACC
PRIMER_LEFT=436,22
PRIMER_RIGHT=550,22
PRIMER_LEFT_TM=60.280
PRIMER_RIGHT_TM=59.917
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=0.3892
PRIMER_LEFT_1_PENALTY=0.306698
PRIMER_RIGHT_1_PENALTY=0.082508
PRIMER_LEFT_1_SEQUENCE=GAACGTTGAACTTGTGTTTTCG
PRIMER_RIGHT_1_SEQUENCE=GGTTTCCTTCCTATATCCCACC
PRIMER_LEFT_1=313,22
PRIMER_RIGHT_1=550,22
PRIMER_LEFT_1_TM=59.693
PRIMER_RIGHT_1_TM=59.917
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.6604
PRIMER_LEFT_2_PENALTY=0.279852
PRIMER_RIGHT_2_PENALTY=0.380537
PRIMER_LEFT_2_SEQUENCE=GCATGGGGAGAATGAAATTAGA
PRIMER_RIGHT_2_SEQUENCE=GGAGCATTCACAACGTTATCCT
PRIMER_LEFT_2=436,22
PRIMER_RIGHT_2=587,22
PRIMER_LEFT_2_TM=60.280
PRIMER_RIGHT_2_TM=60.381
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.6872
PRIMER_LEFT_3_PENALTY=0.306698
PRIMER_RIGHT_3_PENALTY=0.380537
PRIMER_LEFT_3_SEQUENCE=GAACGTTGAACTTGTGTTTTCG
PRIMER_RIGHT_3_SEQUENCE=GGAGCATTCACAACGTTATCCT
PRIMER_LEFT_3=313,22
PRIMER_RIGHT_3=587,22
PRIMER_LEFT_3_TM=59.693
PRIMER_RIGHT_3_TM=60.381
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.0000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=0.9419
PRIMER_LEFT_4_PENALTY=0.306698
PRIMER_RIGHT_4_PENALTY=0.635171
PRIMER_LEFT_4_SEQUENCE=GAACGTTGAACTTGTGTTTTCG
PRIMER_RIGHT_4_SEQUENCE=TCTTCTCTGTGGTTTCCTTCCT
PRIMER_LEFT_4=313,22
PRIMER_RIGHT_4=560,22
PRIMER_LEFT_4_TM=59.693
PRIMER_RIGHT_4_TM=59.365
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_00684 Ps_contig_mira-and-tgicl-ass_franssen_6557
SEQUENCE=AAAAAGAAGGGAAGGAAAATGGGCAGCTGGGCTATAGGACAATCTCATTTGAAGGCAGGCACCATCTGGATCACTTGGATAATCAGAAGCTAGTGTATTGGCAAAATGAATGTCAGCTTCAAGAGCTTTGAGGGAATCCTTGAAAGACTTTCGCATGATCTCTCTATTTCCTTTTTCTTCCTTCTTCCTTCTTTTGAGAACAAACCCCATTCATAAATCTCTCAATAAAAAAACTGTGGGATAAAAGAACATGATAAAGAACAACATCCTCGTACCAAACCAGAGTAGAAAAAAGAACAGTCTTTCAGATGTAGCGAGAGATCAGAGAGAGAGAGTAAATAGTATAGTTATTAACTAAGAATGTCTTGTGTTGTTGTGTTGGGGGTACTCACTTGAACTACC
TARGET=324,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3378
PRIMER_LEFT_PENALTY=0.149516
PRIMER_RIGHT_PENALTY=0.188254
PRIMER_LEFT_SEQUENCE=CTTTTGAGAACAAACCCCATTC
PRIMER_RIGHT_SEQUENCE=AAGTGAGTACCCCCAACACAAC
PRIMER_LEFT=190,22
PRIMER_RIGHT=393,22
PRIMER_LEFT_TM=59.850
PRIMER_RIGHT_TM=60.188
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=0.3394
PRIMER_LEFT_1_PENALTY=0.151106
PRIMER_RIGHT_1_PENALTY=0.188254
PRIMER_LEFT_1_SEQUENCE=AACAACATCCTCGTACCAAACC
PRIMER_RIGHT_1_SEQUENCE=AAGTGAGTACCCCCAACACAAC
PRIMER_LEFT_1=259,22
PRIMER_RIGHT_1=393,22
PRIMER_LEFT_1_TM=60.151
PRIMER_RIGHT_1_TM=60.188
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=0.5785
PRIMER_LEFT_2_PENALTY=0.390220
PRIMER_RIGHT_2_PENALTY=0.188254
PRIMER_LEFT_2_SEQUENCE=TCCTTCTTTTGAGAACAAACCC
PRIMER_RIGHT_2_SEQUENCE=AAGTGAGTACCCCCAACACAAC
PRIMER_LEFT_2=185,22
PRIMER_RIGHT_2=393,22
PRIMER_LEFT_2_TM=59.610
PRIMER_RIGHT_2_TM=60.188
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.6035
PRIMER_LEFT_3_PENALTY=0.415247
PRIMER_RIGHT_3_PENALTY=0.188254
PRIMER_LEFT_3_SEQUENCE=ACATCCTCGTACCAAACCAGAG
PRIMER_RIGHT_3_SEQUENCE=AAGTGAGTACCCCCAACACAAC
PRIMER_LEFT_3=263,22
PRIMER_RIGHT_3=393,22
PRIMER_LEFT_3_TM=60.415
PRIMER_RIGHT_3_TM=60.188
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=0.6035
PRIMER_LEFT_4_PENALTY=0.415247
PRIMER_RIGHT_4_PENALTY=0.188254
PRIMER_LEFT_4_SEQUENCE=CATCCTCGTACCAAACCAGAGT
PRIMER_RIGHT_4_SEQUENCE=AAGTGAGTACCCCCAACACAAC
PRIMER_LEFT_4=264,22
PRIMER_RIGHT_4=393,22
PRIMER_LEFT_4_TM=60.415
PRIMER_RIGHT_4_TM=60.188
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_00685 Ps_contig_mira-and-tgicl-ass_franssen_6583
SEQUENCE=GGGGGATACTTCTTCTTATTTTCATTTCTAGAGAGAGGAAACTACTTCAACTCAACTCTCAACTCTCAACTCAACTCTCAACAACAACAACTAACTAGTGAGAGAAACTATGGGATCTCAATCTTTCATTTGATTTCATTCTCTCTTCTTCAATCTTCAATCTCTTCAGTTTTAATCTGGTTTCTTTGAGTACTGCAATCGGCGAAGGGAACTAGTTTGTTTGGGCTGATTTGCAAAGACGTGATGGGTTCGAGATTCCCATCTCATCAGCTCAGCAATGGTCTGTATGTATCAGGCCGTCCTGAACAGCCGAAAGAAAAGGCTCCAACGATGAGCTCAGTTGCCATGCCTTATACGGGCGGAGACATCAAGAGGTCGGGAGAGTTAGGGAAAATGTTTGATATACCTATGGATGGCTCTAAATCTAGAAAATCAGGTCCATTAAATAATGCTCCTATAAGGACCGGATCATTTGGAGGCGCTGTTTCGCACTCCGGACCAATTATGCAAAACGCGGCTGCCCGTTCTGCTTATGTCACATCAGGTAATCTATCTGCTGCAGGCATGTCCAC
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.8999
PRIMER_LEFT_PENALTY=3.737673
PRIMER_RIGHT_PENALTY=0.162217
PRIMER_LEFT_SEQUENCE=CTTCAACTCAACTCTCAACTCTCAA
PRIMER_RIGHT_SEQUENCE=CAAACAAACTAGTTCCCTTCGC
PRIMER_LEFT=44,25
PRIMER_RIGHT=222,22
PRIMER_LEFT_TM=59.262
PRIMER_RIGHT_TM=60.162
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=3.9622
PRIMER_LEFT_1_PENALTY=3.866633
PRIMER_RIGHT_1_PENALTY=0.095523
PRIMER_LEFT_1_SEQUENCE=TTCAACTCAACTCTCAACTCTCAAC
PRIMER_RIGHT_1_SEQUENCE=TACAGACCATTGCTGAGCTGAT
PRIMER_LEFT_1=45,25
PRIMER_RIGHT_1=286,22
PRIMER_LEFT_1_TM=59.133
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=4.0289
PRIMER_LEFT_2_PENALTY=3.866633
PRIMER_RIGHT_2_PENALTY=0.162217
PRIMER_LEFT_2_SEQUENCE=TTCAACTCAACTCTCAACTCTCAAC
PRIMER_RIGHT_2_SEQUENCE=CAAACAAACTAGTTCCCTTCGC
PRIMER_LEFT_2=45,25
PRIMER_RIGHT_2=222,22
PRIMER_LEFT_2_TM=59.133
PRIMER_RIGHT_2_TM=60.162
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=4.1682
PRIMER_LEFT_3_PENALTY=4.072692
PRIMER_RIGHT_3_PENALTY=0.095523
PRIMER_LEFT_3_SEQUENCE=CTTCAACTCAACTCTCAACTCTCA
PRIMER_RIGHT_3_SEQUENCE=TACAGACCATTGCTGAGCTGAT
PRIMER_LEFT_3=44,24
PRIMER_RIGHT_3=286,22
PRIMER_LEFT_3_TM=57.927
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=4.2349
PRIMER_LEFT_4_PENALTY=4.072692
PRIMER_RIGHT_4_PENALTY=0.162217
PRIMER_LEFT_4_SEQUENCE=CTCAACTCTCAACTCTCAACTCAA
PRIMER_RIGHT_4_SEQUENCE=CAAACAAACTAGTTCCCTTCGC
PRIMER_LEFT_4=50,24
PRIMER_RIGHT_4=222,22
PRIMER_LEFT_4_TM=57.927
PRIMER_RIGHT_4_TM=60.162
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_00686 Ps_contig_mira-and-tgicl-ass_franssen_6609
SEQUENCE=GACTTCTTATTTTACACACGACTCTCACAAACCTATCATTCACGTGAATCTCCAAAATGGGTTCTGAGAAGAAGGAAAATGGAGTGATTTTGGAGGTGGAACCAAAACCTAGCAATGGGTTCACTTCAAAAGCCGTTGATTTGTTGGAGAAGATTATTGTTAAGCTCTTCTATGATTCTTCACTTCCTCATCACTGGCTTTCTGGTAATTTTGCACCTGTCAAAGACGAGACGCCTCCTGTTAAGGATCTTACCGTGCAAGGCCACCTTCCGGATTGCTTGAATGGAGAGTTTGTTAGGGTGGGGCCCAATCCAAAGTTTTCTCCCGTGGCAGGATATCACTGGTTTGACGGAGATGGAATGATCCATGGTTTGCGTATCAAAGATGGAAAAGCTTCTTATGTTTCCCGCTTCGTGAAAACTTCTCGTTTTAAACAAGAAGAATACTTTAATGGCTCTAAATTTATGAAGATTGGAGATCTCAAAGGTCTATTTGGACTGTTAATGGTTAACATGCAAATGTTAAGAGCTAAATTGAAAATACTGGATGTTTCTTATGGACACGGAACAGCTAATACAGCTCTTGTATATCATCACCAGAAGCTTCTAGCACTCTCGGAAGGAGACAAACCTTATGCTATTAAAGTTTTTGAAGACGGTGATTTGCAGACACTTGGCATGCTAGATTATGACAAGAGATTGGGCCATAACTTCACAGCTCATCCAAAAGTTGACCCATTTACTGGGGAGATGTTTACATTTGGATATTCACATACAGCACCATATGTCACATACAGAGTAATATCAAAGGACGGTTTTATGCAAGATCCCGTTCCCATAACAATATCAGATCCCGTCATGATGCATGACTTTGCCATCACAGAGAATTATTCGATATTTATGGACCTTCCTCTGTACTTTAGGCCAAAGGAAATGGTGAAGAATAAGACCTTGATATTCTCATTTGATTCAACCAAGAAAGCTCGTTTTGGTGTCTTACCTCGGTATGCTAAGGATGATAAGCATATCAGATGGTTTGAACTACCAAATTGCTTCATTTTCCACAATGCCAATGCTTGGGAGGAGGAGGATGAAATTGTTTTGATCACGTGCCGCTTGGAGAATCCAAATTTGGACGTGGTCGGCGGGGCTGTCAAGGAAAAGCTTGATAATTTCTCAAATGAGCTGTATGAGATGAGATTTAACATGAAAACCGGTGAAGCTTCTCAAAAGAAACTATCCGCGTCTACTGTAGATTTTCCTAGGGTGAATGAAAGCTACACTGGCAGGAAGCAACGATACGTGTATGGAACCACATTAGACAGCATTGCTAAAGTTACTGGGATTATTAAATTTGATTTGCACGCTGAGCCGGATTCTGGAAAAACAAAGCTTGAAGTTGGAGGAAATGTTCAAGGTCTTTATGACTTGGGACCAGGAAGGTTTGGCTCTGAGGCTGTTTATGTCCCACGTGTCCCTGGCACCGATTCTGAAGAAGATGACGGATACTTGATCTTTTTTGTACATGATGAGAATACCGGGAAATCATTCGTGCACGTCATAGATGCAAAAACAATGTCAGCGGAACCTGTTGCAGTTGTAGAATTGCCCCAAAGAGTTCCATATGGGTTCCATGCTTTCTTTGTCACTGAGGACCAACTGCAAGAACAGGCTAAGTTCTAAACATGCAGACAACCTCATTGCACTGCCTCAGAATGAATCACGCTTATATTCTAAACTTATAGTTTTATTTATCATATACTTGTATAATTGTAACTTGTTTTAAAGTCACTCTCTAAAGTTAAAGATTAAAGGATTCTGTTATACTATTTTTATATTTATTTGAGAGAGAATTATGAAACCATTTGATTAATATAATAGAAGCGCATATACAGACTTC
TARGET=1079,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4283
PRIMER_LEFT_PENALTY=0.171757
PRIMER_RIGHT_PENALTY=0.256503
PRIMER_LEFT_SEQUENCE=GCTCGTTTTGGTGTCTTACCTC
PRIMER_RIGHT_SEQUENCE=GACGCGGATAGTTTCTTTTGAG
PRIMER_LEFT=980,22
PRIMER_RIGHT=1245,22
PRIMER_LEFT_TM=60.172
PRIMER_RIGHT_TM=60.257
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.4283
PRIMER_LEFT_1_PENALTY=0.171757
PRIMER_RIGHT_1_PENALTY=0.256503
PRIMER_LEFT_1_SEQUENCE=GCTCGTTTTGGTGTCTTACCTC
PRIMER_RIGHT_1_SEQUENCE=AGACGCGGATAGTTTCTTTTGA
PRIMER_LEFT_1=980,22
PRIMER_RIGHT_1=1246,22
PRIMER_LEFT_1_TM=60.172
PRIMER_RIGHT_1_TM=60.257
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.4283
PRIMER_LEFT_2_PENALTY=0.171757
PRIMER_RIGHT_2_PENALTY=0.256503
PRIMER_LEFT_2_SEQUENCE=GCTCGTTTTGGTGTCTTACCTC
PRIMER_RIGHT_2_SEQUENCE=ACGCGGATAGTTTCTTTTGAGA
PRIMER_LEFT_2=980,22
PRIMER_RIGHT_2=1244,22
PRIMER_LEFT_2_TM=60.172
PRIMER_RIGHT_2_TM=60.257
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.4886
PRIMER_LEFT_3_PENALTY=0.232060
PRIMER_RIGHT_3_PENALTY=0.256503
PRIMER_LEFT_3_SEQUENCE=GATTCAACCAAGAAAGCTCGTT
PRIMER_RIGHT_3_SEQUENCE=GACGCGGATAGTTTCTTTTGAG
PRIMER_LEFT_3=965,22
PRIMER_RIGHT_3=1245,22
PRIMER_LEFT_3_TM=59.768
PRIMER_RIGHT_3_TM=60.257
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.4886
PRIMER_LEFT_4_PENALTY=0.232060
PRIMER_RIGHT_4_PENALTY=0.256503
PRIMER_LEFT_4_SEQUENCE=GATTCAACCAAGAAAGCTCGTT
PRIMER_RIGHT_4_SEQUENCE=AGACGCGGATAGTTTCTTTTGA
PRIMER_LEFT_4=965,22
PRIMER_RIGHT_4=1246,22
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=60.257
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_00687 Ps_contig_mira-and-tgicl-ass_franssen_6621
SEQUENCE=GGGATTTTGAATCACATACTACATTAATCTCTTGGTCTCCTTATTATTACCGTCTCCAAAACCTTACBCGCGGACACACAAAAAAAAATCCCATCTTCCTCACACCCCAATCAAATAAACTAGAAGCTTAAATCCCAAATCCTTCATCATCAGCAAACATGTTTACATCATCTTCTATCCAATTCCACGCTCCAATCATTCATCATCGTCTATCCTTCACCACAGCCCACTCCTTATCCTTTCACTCCCACAACCATAACCACACATTCCACACTCCATCATTCCTACGCCCTCCTCCGCTGCTAAACCCCTCCCCTCTTCCCTCCATTTTCACTGTTGCCGCCAAGTCTCCAACACCCGACAAAGCCAGCGAGCAGAAATGGGTCCACGAAGGTCTCATCACTGAATCCCTCCCCAACGGCATGTTCCGCGTTCTCCTCGACAACGAAGACCTTATCCTCGGCTACATCTCCGGAAAGATTCGAAAGAATTATGTTCGGATCCTTCCCGGAGATAGAGTTAGAGTTGAAGTCAGTCGTTATGACTCCTCCAAAGGACGCATAATTTACCGGATTCGAAATACCACTGGTGGTGGCAGCTCTTAGTTTTTAGGCATGTCTAGTGTTTGTGAAAATGACTCAATCAAATTTGTGAGTTTTGTTTGGGTCCTAATCGGGTTTGTTTAAATGGAGGGATGAGTAAAAAGGAAACTTTGTCTATTTGTTTTTTAATTTCAACGTTCCTTGGGTATAAAGAACCAACTTTGAGTACATTGTTGTTGTTGTAGAAAAAGCTAAAAGCTAAAATATGCTTTCTTCTCTCATTTTGCACTGCTTATTTCAGTTTCATCTTTTTAAGTCTTGTCTTGTTTGTAGTTTAGAGGATAACTAAATAAACTATTCAATTTCATTATGGAGTAGTGACGATACAAAGTAGTAGTTTGCAATACTAAAGTGAACACCAAAGGTTTTGTTGTCCGGCAAATTTTCCATTGCTGAAATGCTACCTTCAACACCGAATGGACTCTGCTGCTACCTTCAGCCTCAGGGTGATGGTTATGTAGATAAAGCCTTTCCCAATTTTTAAAATCATTCATTGCAAA
TARGET=773,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00688 Ps_contig_mira-and-tgicl-ass_franssen_6622
SEQUENCE=ACTTGACCGGTAATGGCTATGAGAGGGACTACTGTCCAATCAAAAGCATCAGCAAGTCCGCTGACTAAGTTGGTGGCACCGGGACCGGAAGTGGCGATGCAGACACCGGGGAGACCGGAGGAGCGTGCGTAGCCTTCGGCGGCAAAGACTCCACCTTGTTCGTGGCGGGGAAGTATGTTTCGGATTGTTCTAGAGCGTGTAAGAGCTTGGTGGATCTCCATGGAAGCTCCGCCGGGGTAAGCGAATACGTTGGTGACGCCTTGACGCTCGAGAGCTTCGACGAGAAYGTCGGCTCCTTTTCTTGGCTCGGTGGAGGAGAAACGAGAGATGTATTGATCATCGGAGGTCGTCGTGGTGGTGGTGGTGGAAGGTGGTGGCGGGCGGGGGRRGTTGGAGG
TARGET=352,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00689 Ps_contig_mira-and-tgicl-ass_franssen_6623
SEQUENCE=TTTTTYTKTRGTAGAAATAACGCTCTATAAAAACACATTAACAACAACAACCAAGTCTTATCCTACTAAGTGAAATCGACTACATAGATCAACTTTCACCATAATATTCACTCTATAAAAACACATACCACCAAAAATTACAACAAAATATAATGCTTAACTTACCACATGGAAAATCCTGCAACTATATTGTACAAAACAAACATGAAGGAATATCATATCTTATTTTGAACCAAGAAATCAGTAGCTCGTTCTGCCATCACCCTCTGTGATCACATCCTTGAAAGATCCATTACTTGGAATCATTGGCAATACATGTTCTTGATGAGGTGTAATGACATCAAGAAGATAAGGGCCAGGAGTGTCCAACATTTTCTGAATAGCTTCTCGAAGCTCTTCCTTTTTGGTCACACGAGCTGCCGGTATTCCACAAGCATCAGCAAATCCAAGCATGTTAGGGAAAATCTCATCCTCCTTAGAAGGGTCACCAAGATAAGTATGACCTCTATTTGACTTATAGAATCTATCCTCCCATTGAACAACCATACCCAAATGTTGATTATTCAACAACAATATCTTAATTGGAAGATTCTCCACTCTTATAGTAGCCAACTCTTGAACATTCATGATAAAGCTACCATCCCCATCAATATCAACAACGATCGCATCAGGGTTAGCAACAGCAGCACCAATAGCAGCTGGTAATCCAAAACCCATAGCACCAAGTCCACCGGAAGTTAACCACTGCCTAGGTCTCTTATACTCATAAAACTGAGCAGCCCACATTTGATGCTGTCCAACACCAGTACTAATAATAGCATCTCCATTAGTCAATTCATCAAGTACCTGAATAGCATACTGCGGAGAAATCGCATTCTCAAACGTCTTAAACCCAAGAGGAAATTTCAATTTCTGAACATTCAACTCCTGCCTCCATGCTTCAAAATCAAGTTTACCCTTAACTCCTTTACTCTCCAAAACCCTATTAAGACCTTCCAATGCCACCTTCATATCAGCACAGATCGACAAATGTGGAATCTTATTCTTCCCAATCTCAGCAGAATCAATATCTATATGAACAATCTTAGCCCTACTAGCAAAAGCTTCTAACTTTCCGGTTACACGATCGTCAAACCTAACCCCGAAAGCAAGCAACAGATCACTACTATCAACAGCATAATTAGCATAAACAGTACCATGCATACCCAACATGTGAAGGGAATGTTCAGCTCCAATAGGGTAACTACCAAGTCCCATTAAAGTACTAGCAACAGGAATACCGGTCAGTTCAACAAACCGGTTCAATTCCTCACTGGAATTCAAACATCCACCTCCAACATACAAAACTGGTTTTTTAGATTCTAATAACAACCTCAGAACCTGTTCAAGTTGGGCCTCGCTAGGAATTTTGGGTAACCTGGAAACATACCCAGTGAGCCTGATGGGCTCGGCCCAATTAGGAACTGCTAACTGTTGTTGAATGTCTTTGGGTAGGTCGATGAGAACCGGGCCGGGCCTGCCTGAAGTTGCAAGGAAAAAAGCCTCTTTCACAACCCTAGGAATGTCATCAACATCGAGAATGAGGTAGTTATGCTTTGTGATGGATCTCGTTACTTCAACGATTGGGGTTTCTTGAAAAGCATCGGTTCCAATCATCCTCCGGGGAACTTGACCGGTAATGGCTATGAGAGGGACACTGTCCATCAAAGCATCAGCAAGTCCGCTGACTAAGTGGTGGCGACCGGGACCGGAAGG
TARGET=40,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00690 Ps_contig_mira-and-tgicl-ass_franssen_6659
SEQUENCE=GTTGGTTGCATCCCTGATACAGCTCTCACTTCTCCCACACTCTCACCGGTTATCTCTTTTGGCATAAGTGTTTTTGGAATGATTCCTAACACGTGACATCCACCATCATAAACAACTTGCGAGATACGACCCATCAATCCTATACTTCCTCCTCCATACACCAAGTCAATCTTCCTTTCCACCAATTGTTTTCCGAGCTGAATAGCAGCAATTTGGTAGCTAGGATTTTTTTCCTGGACTGCTTCCACAGTAAACACATATGCTTTTGAATCTAGACTTGATGAGGATGGGTACTTGCTGCTCAATTTCCATAAAGATTAAGAGAATAAGCGATTTTTTGGGGTTAAGAAATTAAGGAAGGGACAAAGAGAATTGGATAGAAGGTAATGAAGTGATATGTSAGAATGAATGAATGAAAGAAGACACTCAAGAAGNGTAGTCAGCAATCATGATTCCAAGTCCCGNGTATNTG
TARGET=403,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00691 Ps_contig_mira-and-tgicl-ass_franssen_6699
SEQUENCE=GGTCGTCTTCCACTTTTTGGTTAGTTCTCTCATGTCATTGGTTGGTAGGCATTCTAACTATCACGGCTTCTATTCTGCTGCCTTGCACGTGTTACTTGAAGATTTCTGGAAGTTATAGAAAAATTGGATTGAAACAATAGCAATAGTGACAATAATATTAGTAGCTATAGTGATGGGAATTTCAAGGAACATACACTTCAATTGTTGAATTGGTACAAAAGTCTTCTAACAACTAGAAGATTTATATATGATGATGGGATAAATTTATAAACAACCACTTGTTACAAATGTAAAAGGTCTTGATCGCATGATTTATCAATCATATAATTATCTGCGCGCGTGATGGCTTTGTTGAATTGTCGTTATTTATTTATTTTTATTAATTTTAAACATTTGTTAGTAAGAAAAAAACT
TARGET=363,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00692 Ps_contig_mira-and-tgicl-ass_franssen_6704
SEQUENCE=CTATTTTGATGTTCCTTGCTTTCGATAAATATGGACTTTCAACAAACAAACAAACCAAAGTCTTATCCCATTAAATGGTGTTCGGCTACTATGGATCAAAATTTCACTATAATTTTTATCCTCTAAA
TARGET=41,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=8.1848
PRIMER_LEFT_PENALTY=3.108135
PRIMER_RIGHT_PENALTY=5.076662
PRIMER_LEFT_SEQUENCE=TTTGATGTTCCTTGCTTTCG
PRIMER_RIGHT_SEQUENCE=GTGAAATTTTGATCCATAGTAGCCG
PRIMER_LEFT=4,20
PRIMER_RIGHT=106,25
PRIMER_LEFT_TM=58.892
PRIMER_RIGHT_TM=62.077
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.0000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=103
PRIMER_PAIR_PENALTY_1=8.7785
PRIMER_LEFT_1_PENALTY=3.701882
PRIMER_RIGHT_1_PENALTY=5.076662
PRIMER_LEFT_1_SEQUENCE=TGATGTTCCTTGCTTTCGAT
PRIMER_RIGHT_1_SEQUENCE=GTGAAATTTTGATCCATAGTAGCCG
PRIMER_LEFT_1=6,20
PRIMER_RIGHT_1=106,25
PRIMER_LEFT_1_TM=58.298
PRIMER_RIGHT_1_TM=62.077
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.3000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=101
=
PRIMER_SEQUENCE_ID=SSR_00693 Ps_contig_mira-and-tgicl-ass_franssen_6706
SEQUENCE=GTACATGCGAGACAAAATTGAATCATTTTTTAGGTTGTGGTGATTGTTGGATATGGTTCTGAAAATGGTGTGGATTATTGGTTGTTGAGGAAACTCATGGGGCACTAAATGGGGGTGAAGATGGTTACTTCAAGTTAGAGAGTAATATAGAAAAACACAGTTAGAGGCAACTGTGGAATCGCAATGTATCCTTCATACCCAATGAAGTATGGTCCAAATTCTGTCGGTTTTTACAAGGGGTAATGAAGTATGGTCCAAATTCTGCGGTTCTTTTAACTAAGGGTTAGGGATGTGCATGGATCAATAAACCAAATTAAATTAAATTATATATACATTGGTTTCGGTTTNAATCTTAAAAACCNTTTCAATTAAAACCTAATTTTATTTTACTAAAACCG
TARGET=311,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00694 Ps_contig_mira-and-tgicl-ass_franssen_6727
SEQUENCE=CCGTTTTTTTTAAAACTTTAAAAGTANAACGGACCCGTTTCCAACTTAAGNTAAAAATAATCAATTGTTACAATATCTACTCACTNAGAGNTCTGCTGCAACAAACAATAACACAAACTAAATAGCTTCAACACACATGTATAGTAATTTCATCATCTCTACTTAGACCGTTACAGACTCAAGTTTCACAACACATCCAAGATTACGTGCTCGCAGTAGCTTCTTCAACGACGCCTTCTTTTCCAACACCAGAACCTCCCTCCTTATCATCACCCTCTTCTTTTCCAGAATTTGACACTTCTTGCCCTTCATCCTCACTGTCATCAAAATCATCACCGACAGCATCGTCACCCATCCCGCCAAATTGCGAGAAATCCATCCCTCCCATGTCTAGGTCACCGGTACCCGCATCTTCATCTTCATCCACCCATTTATCCCAATCTACTTTCACGTAATGTGGAGGCTTCCCTTCTGCTTTCAGTAACCTTTTCCACCACTCTTCTTCTGCTTTCTGCACAACACAGAATATGCTTCTCACCCCTACATTAATTTTGCTCTCCTCTACATTAACCTTGTCAAAGAGTGGCAAGTTAAGCTCATACAGATGGTCTCCAGCACCAGCAGTGGCAGAAAAAGTAAAAACACCATCTGGAGTAAGATCCACTTTCGCATTTTTGGTAATCTGGCAACTGAACCGTGACGTAAACTTTGTCGCCCCTTTGAGCCCACTTGATCTCCGGATGACGACTCATATTTGTTTGAGAGAAGAAGAAGAAGCTGTGAAACCCTAGATTCTTTGGTAACTGACTGTCAGAGACAATTGTAACCTCGTTTTCGGATTTTGACGATGATGCAATGCAAAACCTGTTGTTTCTAGATTGTTCTTCTTTTCACTAAATTGCGTAACC
TARGET=764,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2631
PRIMER_LEFT_PENALTY=0.141511
PRIMER_RIGHT_PENALTY=0.121635
PRIMER_LEFT_SEQUENCE=TCATACAGATGGTCTCCAGCAC
PRIMER_RIGHT_SEQUENCE=CAAAATCCGAAAACGAGGTTAC
PRIMER_LEFT=596,22
PRIMER_RIGHT=843,22
PRIMER_LEFT_TM=60.142
PRIMER_RIGHT_TM=59.878
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.3310
PRIMER_LEFT_1_PENALTY=0.209343
PRIMER_RIGHT_1_PENALTY=0.121635
PRIMER_LEFT_1_SEQUENCE=ACCTTGTCAAAGAGTGGCAAGT
PRIMER_RIGHT_1_SEQUENCE=CAAAATCCGAAAACGAGGTTAC
PRIMER_LEFT_1=569,22
PRIMER_RIGHT_1=843,22
PRIMER_LEFT_1_TM=60.209
PRIMER_RIGHT_1_TM=59.878
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.3369
PRIMER_LEFT_2_PENALTY=0.215215
PRIMER_RIGHT_2_PENALTY=0.121635
PRIMER_LEFT_2_SEQUENCE=TTAACCTTGTCAAAGAGTGGCA
PRIMER_RIGHT_2_SEQUENCE=CAAAATCCGAAAACGAGGTTAC
PRIMER_LEFT_2=566,22
PRIMER_RIGHT_2=843,22
PRIMER_LEFT_2_TM=59.785
PRIMER_RIGHT_2_TM=59.878
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.3369
PRIMER_LEFT_3_PENALTY=0.215215
PRIMER_RIGHT_3_PENALTY=0.121635
PRIMER_LEFT_3_SEQUENCE=TAACCTTGTCAAAGAGTGGCAA
PRIMER_RIGHT_3_SEQUENCE=CAAAATCCGAAAACGAGGTTAC
PRIMER_LEFT_3=567,22
PRIMER_RIGHT_3=843,22
PRIMER_LEFT_3_TM=59.785
PRIMER_RIGHT_3_TM=59.878
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.3984
PRIMER_LEFT_4_PENALTY=0.276812
PRIMER_RIGHT_4_PENALTY=0.121635
PRIMER_LEFT_4_SEQUENCE=CTCATACAGATGGTCTCCAGCA
PRIMER_RIGHT_4_SEQUENCE=CAAAATCCGAAAACGAGGTTAC
PRIMER_LEFT_4=595,22
PRIMER_RIGHT_4=843,22
PRIMER_LEFT_4_TM=60.277
PRIMER_RIGHT_4_TM=59.878
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_00695 Ps_contig_mira-and-tgicl-ass_franssen_6751
SEQUENCE=CATGATGAAGATGAGMCAACCGACACTGCAGGTATTGATACTACAACTATGAAGAGAGCAGGCAGGAAGGGAGTCTCCTTCCTTTTCTTCCCTATTCTTATTGCTTTATGTGCTTTCTTGTGGGTTTCTATTGGAAATTTAAGACTATTAGGGACATACGTCTCTCCTTGAATCTGTAAACCCAAAGTTTTGCTGCTATATATATAATTTTGTCTGAGATCTCCTCAGAGTTGATGAAAGCAATCTTCTAACATATTCTAGAAAAATAAAAATATTTTATCCGTTGTAAATATTCAACTCAAGATATGGTTTAGGGTAAGTCACACTCATCTGTAATTCATGAGTAGAGGCACTAAACTTATATAATCTTATTGTTGTTTGATTTTTTGAATTCTTCATAATATCTTTGATTGTACAAATTTGTGAAATATGTACATAGATGTGTATCTATATGCCAGCCAATTTTGTAACCGTCTCTAATTGGTAGTAACTTCTTCAGCATTAATATTATATATTGTTGATRACGT
TARGET=197,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00696 Ps_contig_mira-and-tgicl-ass_franssen_6753
SEQUENCE=TTTTTCGTTATTTGGAAATTTAAAGTAACTATTAGGGACGATACGTCTCTCCTTGAATCTGTAAACCCAAAGTTTTAGTCTGCTATATATATATAATTTTGTTCTGAGATCTCCTCAGAGTTGATGAAAGCAATCTTCTAACATATTCTGAAAATAAAAATAATTTTATCCGTTGTAAATATTCAACTCAAGATATGGTTTAGGGTAAGTCACACTCATCTGTAATTACATGAGTAGAGGCAACTAAACTTATATAATCTTATTGTTGTTTGATTTTTTGAATTCTTCATAATATCTTGATTAGTACAAATTTGNGAAATNTGTACATAGATGTGTATCTATATGCCAGTA
TARGET=84,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8855
PRIMER_LEFT_PENALTY=0.629991
PRIMER_RIGHT_PENALTY=1.255555
PRIMER_LEFT_SEQUENCE=GGACGATACGTCTCTCCTTGAA
PRIMER_RIGHT_SEQUENCE=GATTGCTTTCATCAACTCTGAGG
PRIMER_LEFT=35,22
PRIMER_RIGHT=134,23
PRIMER_LEFT_TM=60.630
PRIMER_RIGHT_TM=60.256
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=2.9438
PRIMER_LEFT_1_PENALTY=0.629991
PRIMER_RIGHT_1_PENALTY=2.313789
PRIMER_LEFT_1_SEQUENCE=GGACGATACGTCTCTCCTTGAA
PRIMER_RIGHT_1_SEQUENCE=TGAGTGTGACTTACCCTAAACCA
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=217,23
PRIMER_LEFT_1_TM=60.630
PRIMER_RIGHT_1_TM=58.686
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=2.9572
PRIMER_LEFT_2_PENALTY=1.701644
PRIMER_RIGHT_2_PENALTY=1.255555
PRIMER_LEFT_2_SEQUENCE=GGACGATACGTCTCTCCTTGA
PRIMER_RIGHT_2_SEQUENCE=GATTGCTTTCATCAACTCTGAGG
PRIMER_LEFT_2=35,21
PRIMER_RIGHT_2=134,23
PRIMER_LEFT_2_TM=59.298
PRIMER_RIGHT_2_TM=60.256
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=100
PRIMER_PAIR_PENALTY_3=3.1090
PRIMER_LEFT_3_PENALTY=1.853395
PRIMER_RIGHT_3_PENALTY=1.255555
PRIMER_LEFT_3_SEQUENCE=GGACGATACGTCTCTCCTTGAAT
PRIMER_RIGHT_3_SEQUENCE=GATTGCTTTCATCAACTCTGAGG
PRIMER_LEFT_3=35,23
PRIMER_RIGHT_3=134,23
PRIMER_LEFT_3_TM=60.853
PRIMER_RIGHT_3_TM=60.256
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=3.2785
PRIMER_LEFT_4_PENALTY=2.022900
PRIMER_RIGHT_4_PENALTY=1.255555
PRIMER_LEFT_4_SEQUENCE=GGGACGATACGTCTCTCCTTG
PRIMER_RIGHT_4_SEQUENCE=GATTGCTTTCATCAACTCTGAGG
PRIMER_LEFT_4=34,21
PRIMER_RIGHT_4=134,23
PRIMER_LEFT_4_TM=61.023
PRIMER_RIGHT_4_TM=60.256
PRIMER_LEFT_4_GC_PERCENT=57.143
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_00697 Ps_contig_mira-and-tgicl-ass_franssen_6756
SEQUENCE=CGGGAACGTTAAGTTGGGAACTTGTCCTAATTAAAAAAGCAAAAGCAAAAGCAAAGAATTTGCAAAACTGCAAAAACCGATTAAAACTCTTTCTTTCCGTTAAACTCTTTCTTGTTGTTGTTGTTGTTGTTGTGAAACGCAACAGAATATTGTTATTGTTGTTCTGCTATCAATGGAGTTCGATCCGATCCCAATCGGTTCGTGCTCCAAAGATCACCACAACATTTATCAAAACTGGTTTTCGGATGAAATCGGTGTCGTAGGGTTG
TARGET=112,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5950
PRIMER_LEFT_PENALTY=0.440098
PRIMER_RIGHT_PENALTY=0.154878
PRIMER_LEFT_SEQUENCE=CGTTAAGTTGGGAACTTGTCCT
PRIMER_RIGHT_SEQUENCE=TCGAACTCCATTGATAGCAGAA
PRIMER_LEFT=6,22
PRIMER_RIGHT=182,22
PRIMER_LEFT_TM=59.560
PRIMER_RIGHT_TM=59.845
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.7218
PRIMER_LEFT_1_PENALTY=0.566962
PRIMER_RIGHT_1_PENALTY=0.154878
PRIMER_LEFT_1_SEQUENCE=ACGTTAAGTTGGGAACTTGTCC
PRIMER_RIGHT_1_SEQUENCE=TCGAACTCCATTGATAGCAGAA
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=182,22
PRIMER_LEFT_1_TM=59.433
PRIMER_RIGHT_1_TM=59.845
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=1.1023
PRIMER_LEFT_2_PENALTY=0.440098
PRIMER_RIGHT_2_PENALTY=0.662154
PRIMER_LEFT_2_SEQUENCE=CGTTAAGTTGGGAACTTGTCCT
PRIMER_RIGHT_2_SEQUENCE=ATCGGATCGAACTCCATTGATA
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=188,22
PRIMER_LEFT_2_TM=59.560
PRIMER_RIGHT_2_TM=60.662
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=1.2291
PRIMER_LEFT_3_PENALTY=0.566962
PRIMER_RIGHT_3_PENALTY=0.662154
PRIMER_LEFT_3_SEQUENCE=ACGTTAAGTTGGGAACTTGTCC
PRIMER_RIGHT_3_SEQUENCE=ATCGGATCGAACTCCATTGATA
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=188,22
PRIMER_LEFT_3_TM=59.433
PRIMER_RIGHT_3_TM=60.662
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=1.4817
PRIMER_LEFT_4_PENALTY=0.440098
PRIMER_RIGHT_4_PENALTY=1.041614
PRIMER_LEFT_4_SEQUENCE=CGTTAAGTTGGGAACTTGTCCT
PRIMER_RIGHT_4_SEQUENCE=AATGTTGTGGTGATCTTTGGAG
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=225,22
PRIMER_LEFT_4_TM=59.560
PRIMER_RIGHT_4_TM=58.958
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_00698 Ps_contig_mira-and-tgicl-ass_franssen_6764
SEQUENCE=TTTAAAGAACGAAAGAAAGAAAAATAGACTTGCTGGTGTTAAACCCTTGTTATCATCCGCCGTCTCCAAGAATTTCCTCCGGCAAGCAAGCTAGATCCTAGTGTCTATGGAGACCAAAACAGTTCCATCCAAGAAAAGCACATAGCAAATAGTTTAGATGGGTTCACAATAGATGAGGCTATGCAAAGGAATAAACTCTATATATTAGACCATCATGATGCTCTGATGCCATACCTAGGTAGAATAAACTCTACCAACACCAAGACTTATGCTACCAGAACAGTTCTATTTCTACAAGATGATGGCACATTGAAGCCACTGGCT
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00699 Ps_contig_mira-and-tgicl-ass_franssen_6774
SEQUENCE=TRYTCGGGGACCCATCTCTCTCTATCTCTCTCGTTTACAACCTTTTCACGTGTCTCTCCCTTTCACACTTGTGTTCTTTTCTGTCTTTGTTTCTTCCCTCTGTTTTTTCCTAGGAAAACCATTTCAATTGGGGCGGTGGTTAAGGTTAATGTTTCTCTGTTTTCTTGTTGAAACTTCGAGGCGAAATAAATAAATAATGGAAGGAAGAAAGCAATTGGGTTCCTCTTCTTCATCTTCTTCTTTCACTTCTGAACTCTTTGGTTCCATTGATTTTCAACCTTCCTCTGCTTCTTCTGGAATTTTCGATTCCCTTTTTTCTCCTTCTTCTAAGGTGTTTGGAAGAGAGTCTCTCCATTCTGTACTGAATGGAAAAACTGCTACTGAGGGATCAAACTCCAAAATTGCTACTCAAGATTATATGAGTAAGGGAGTGAGGGTGAAACTGAGAACAAACAAATAAGGATTTAGAGTTATCTTTATACAAAGAAACGAAAGGGGTTTACCAAAACCCGTAAGTTTACGGGTTACCCGAAACGGGTTACCCGTTTTTTAAACGGGTTTTTAACCCGTAAACGTTTAACCCGTAAAAAACGTTTAACCCGTTTTAAAAAGGTTTTTTAAAACGGGTTTACGGGGGGGGTTTTAACCGGGGGGTAACCCCCGGTAAAAGGGGGGTTAAACGTTTTACGGGGTTTTAAAAACGTTTTTAAACCCCTTTTTTTTAACCCGTAAAACCGGTTTTTAACCCCCCCGTTTTAACCCGGTTAAAACCCGTTTACGGGGGTAAAACGGGTAAAACCCCGGTAAACCCCGGGGTTACCCGTTTTAACGGGGGTAAAACCGGG
TARGET=184,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00700 Ps_contig_mira-and-tgicl-ass_franssen_6783
SEQUENCE=CTATTAAATTGGYCTATTTTTCTTTCACCGCAATGCTATTTGTTTATGGACGTATTCTGAGTCAGCGCCTGGTTAACACAGTAAATTCCGAACAAGGTTTTATATCGGCTTGTGAGCAGTCTTATAAAGTATCATATGGTTGTCTGCTATTCCCTGTACATTACAGGATTCATATGGTTCATTCTCACATTGAAAAAGAAGATGTACAAGTACCAATTTGGCCAGTATGCTTGGACACACATGATTCTGATTGTTGTATTTGGGCAGTCCTCTTTCACCGTGCCAAGCATTTTTGAAGGGATTTTCTGGTTTCTTCTTCCTGCAACGCTTATTGTCATTAATGACATTGCTGCTTATTTCTTTGGTTTCTTCTTTGGAAAAACCCCTTTGATTAAGTTGTCACCAAAGAAAACCTGGGAGGGTTTTATCGGGGCATCTGTTACAACCATCATATCTGCATTCATGCTTGCCAACATCATGGGTCGTTCTCCGTGGTTTACATGTCCCAGGAAGGACCTATCAACTGGTTGGCTTGACTGTGATCCTGGTCTACTGTTTAAGCCAGAATCTTACTCGTTGTTAGGATGGACTCCTCACTGGTTTCCTTGGAAAGAGATATCAATCCTACCAGTTCAATGGCATGCTTTATGTCTTGGCTTGTTTGCTTCAATTATCGCACCTTTCGGAGGCTTCTTTGCAAGTGGTTTCAAAAGGGCTTTTAAAATAAAGGATTTTGGTGATAGTATTCCAGGACACGGTGGAATTACCGACAGAATGGACTGCCAGATGGTTATGGCCGTGTTTGCATATATATATACAATCAGTCCTTTGTCGTGCCAACTAAAGTTTATCGGTTAGAAATGTATTTTAGGACCAAG
TARGET=807,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00701 Ps_contig_mira-and-tgicl-ass_franssen_6801
SEQUENCE=AAAAAGTACTTTTTWAWTAAWTTGAAGCAAGCTGTCTGTAATTAATTGGATTTCAGAAGTTGTTATGTACAAGAATGGAAGAGCTAGCTAGACCTTTGTAAGAAAGTCCCTCTTTTAGTTAATACAGATGAAAAAGTCAGTCTTGAAGTCTTTTTTGATATTGTTGCCTTTGGACTATTATACCATATTCTATCACCATAACTTCTACATGACTTCATTCTTTTATTGCTTTTCTTTGGAACATCTTCCATCTTTTCTACCTCACCTAAGCAACTGAATGATTGTGTCTTCCCTTCATAGAACATCGACAACCCTCTCTTTATTGGAAGATTGTTCATGAGCTCTGATAAGTCACATAAAGATCCATTTGAATGTGATGATGAAGTTGAAGATGAATCAGCATCCTCTATCAACTCAGATGAACAAGAACACAAACAATTCATTGAATCTTCTGAAAATGTTCCAATGGATGATGATGATGATGATGTTGTTGAATCACTTTTATTTTCCTTTACCATTATTCCTTTAACATTCATTTCAACAAAGATTTGTATCAAGAAGAAGAAATGAAACAATCCTTCAATGAAAAAGCTTAGATCCGTTATGTTATATTTATAAAAATATTGGATCTTATATATATAAGAATAATTGGATAACATTGACTAATTTTATCCCTTTTAGTATAGTTTACTTAAAAGGTCATTATTATATTAAATTTTGYCSCKAYGAGATAAATGTTATATCCCGA
TARGET=469,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00702 Ps_contig_mira-and-tgicl-ass_franssen_6801
SEQUENCE=AAAAAGTACTTTTTWAWTAAWTTGAAGCAAGCTGTCTGTAATTAATTGGATTTCAGAAGTTGTTATGTACAAGAATGGAAGAGCTAGCTAGACCTTTGTAAGAAAGTCCCTCTTTTAGTTAATACAGATGAAAAAGTCAGTCTTGAAGTCTTTTTTGATATTGTTGCCTTTGGACTATTATACCATATTCTATCACCATAACTTCTACATGACTTCATTCTTTTATTGCTTTTCTTTGGAACATCTTCCATCTTTTCTACCTCACCTAAGCAACTGAATGATTGTGTCTTCCCTTCATAGAACATCGACAACCCTCTCTTTATTGGAAGATTGTTCATGAGCTCTGATAAGTCACATAAAGATCCATTTGAATGTGATGATGAAGTTGAAGATGAATCAGCATCCTCTATCAACTCAGATGAACAAGAACACAAACAATTCATTGAATCTTCTGAAAATGTTCCAATGGATGATGATGATGATGATGTTGTTGAATCACTTTTATTTTCCTTTACCATTATTCCTTTAACATTCATTTCAACAAAGATTTGTATCAAGAAGAAGAAATGAAACAATCCTTCAATGAAAAAGCTTAGATCCGTTATGTTATATTTATAAAAATATTGGATCTTATATATATAAGAATAATTGGATAACATTGACTAATTTTATCCCTTTTAGTATAGTTTACTTAAAAGGTCATTATTATATTAAATTTTGYCSCKAYGAGATAAATGTTATATCCCGA
TARGET=632,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00703 Ps_contig_mira-and-tgicl-ass_franssen_6812
SEQUENCE=CATTGGAATATCAGAAAATATTGAATGAGAAAATTAGGCCTTCTCTTCAAGATATTGTTTGGGTTTGGCAAGGTGAAATACAAGAACAATCACAAGAGTCGGAGCAGGAGCAGTAGCAGCAGCAGCAACAATCGTGCTATCTTCATTTTGTACATTTCAGGATATACCAGTTTTTTTCCCGAAATTATGCTGTTTCTGCATAGCTTTTTATGTGTTTAACTATGACATAACCTTATTGTATTAGGTTTGTATGTTGAGTGTGATGGTTAACAAACAACCAGTGTACCAAAGACATGCTAGAACATGTTCAAAGTTCTTGAGAAATAAAGTTCTGGGTTGAAGATTGACTAAA
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1941
PRIMER_LEFT_PENALTY=0.225996
PRIMER_RIGHT_PENALTY=0.968095
PRIMER_LEFT_SEQUENCE=CAAGAACAATCACAAGAGTCGG
PRIMER_RIGHT_SEQUENCE=GCATGTCTTTGGTACACTGGTT
PRIMER_LEFT=80,22
PRIMER_RIGHT=296,22
PRIMER_LEFT_TM=59.774
PRIMER_RIGHT_TM=59.032
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=1.2480
PRIMER_LEFT_1_PENALTY=0.225996
PRIMER_RIGHT_1_PENALTY=1.022025
PRIMER_LEFT_1_SEQUENCE=CAAGAACAATCACAAGAGTCGG
PRIMER_RIGHT_1_SEQUENCE=AGCATGTCTTTGGTACACTGGTT
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=297,23
PRIMER_LEFT_1_TM=59.774
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=218
PRIMER_PAIR_PENALTY_2=1.4919
PRIMER_LEFT_2_PENALTY=0.225996
PRIMER_RIGHT_2_PENALTY=1.265933
PRIMER_LEFT_2_SEQUENCE=CAAGAACAATCACAAGAGTCGG
PRIMER_RIGHT_2_SEQUENCE=GCAGAAACAGCATAATTTCGG
PRIMER_LEFT_2=80,22
PRIMER_RIGHT_2=198,21
PRIMER_LEFT_2_TM=59.774
PRIMER_RIGHT_2_TM=59.734
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=119
PRIMER_PAIR_PENALTY_3=1.5308
PRIMER_LEFT_3_PENALTY=0.225996
PRIMER_RIGHT_3_PENALTY=1.304785
PRIMER_LEFT_3_SEQUENCE=CAAGAACAATCACAAGAGTCGG
PRIMER_RIGHT_3_SEQUENCE=CAGAAACAGCATAATTTCGGGA
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=197,22
PRIMER_LEFT_3_TM=59.774
PRIMER_RIGHT_3_TM=61.305
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=1.5447
PRIMER_LEFT_4_PENALTY=0.576589
PRIMER_RIGHT_4_PENALTY=0.968095
PRIMER_LEFT_4_SEQUENCE=GATATTGTTTGGGTTTGGCAAG
PRIMER_RIGHT_4_SEQUENCE=GCATGTCTTTGGTACACTGGTT
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=296,22
PRIMER_LEFT_4_TM=60.577
PRIMER_RIGHT_4_TM=59.032
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_00704 Ps_contig_mira-and-tgicl-ass_franssen_6832
SEQUENCE=AAAATTTAGTCTCGGNGAAAGAAGACTGATAGAGACCTCAGCTAATCAATTCCATACATTCTCAATAAATTTTTGTGAAAATAGGATTGATGTTACTTACTGCGAACAGAATTGTCACGATGAACAAAAAGATTCGGATGAATATTCTCATCATCATCAAATGTTGTCCACTGCCTCGAGTTCTCACTGTGTTTTTCTTTTGCTATGCCGCGCATGTGTTCCATTT
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9262
PRIMER_LEFT_PENALTY=0.069627
PRIMER_RIGHT_PENALTY=0.856575
PRIMER_LEFT_SEQUENCE=AGTCTCGGNGAAAGAAGACTGA
PRIMER_RIGHT_SEQUENCE=AAACACAGTGAGAACTCGAGGC
PRIMER_LEFT=7,22
PRIMER_RIGHT=193,22
PRIMER_LEFT_TM=59.930
PRIMER_RIGHT_TM=60.857
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=1.0436
PRIMER_LEFT_1_PENALTY=0.069627
PRIMER_RIGHT_1_PENALTY=0.973959
PRIMER_LEFT_1_SEQUENCE=AGTCTCGGNGAAAGAAGACTGA
PRIMER_RIGHT_1_SEQUENCE=CGGCATAGCAAAAGAAAAACAC
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=209,22
PRIMER_LEFT_1_TM=59.930
PRIMER_RIGHT_1_TM=60.974
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=1.5558
PRIMER_LEFT_2_PENALTY=0.069627
PRIMER_RIGHT_2_PENALTY=1.486178
PRIMER_LEFT_2_SEQUENCE=AGTCTCGGNGAAAGAAGACTGA
PRIMER_RIGHT_2_SEQUENCE=AACACAGTGAGAACTCGAGGC
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=192,21
PRIMER_LEFT_2_TM=59.930
PRIMER_RIGHT_2_TM=59.514
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=1.5889
PRIMER_LEFT_3_PENALTY=0.732298
PRIMER_RIGHT_3_PENALTY=0.856575
PRIMER_LEFT_3_SEQUENCE=GTCTCGGNGAAAGAAGACTGAT
PRIMER_RIGHT_3_SEQUENCE=AAACACAGTGAGAACTCGAGGC
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=193,22
PRIMER_LEFT_3_TM=59.268
PRIMER_RIGHT_3_TM=60.857
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.50
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=1.7063
PRIMER_LEFT_4_PENALTY=0.732298
PRIMER_RIGHT_4_PENALTY=0.973959
PRIMER_LEFT_4_SEQUENCE=GTCTCGGNGAAAGAAGACTGAT
PRIMER_RIGHT_4_SEQUENCE=CGGCATAGCAAAAGAAAAACAC
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=209,22
PRIMER_LEFT_4_TM=59.268
PRIMER_RIGHT_4_TM=60.974
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.50
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_00705 Ps_contig_mira-and-tgicl-ass_franssen_6836
SEQUENCE=TCATTGGCTAAGTCTTTTGATTTTGCTTCACCTTTTTCTTGTTCTCCTGTCTTCTCCCCTTTGAAATTCTATTCTTCTTCTTCTTCTCCTTTTCTTAATTCTTCTATTACAATGCAAACTCCAAACCCTGATTCTTTGGG
TARGET=72,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6445
PRIMER_LEFT_PENALTY=0.143960
PRIMER_RIGHT_PENALTY=0.500551
PRIMER_LEFT_SEQUENCE=TCTTGTTCTCCTGTCTTCTCCC
PRIMER_RIGHT_SEQUENCE=AAGAATCAGGGTTTGGAGTTTG
PRIMER_LEFT=36,22
PRIMER_RIGHT=135,22
PRIMER_LEFT_TM=59.856
PRIMER_RIGHT_TM=59.499
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=0.6445
PRIMER_LEFT_1_PENALTY=0.143960
PRIMER_RIGHT_1_PENALTY=0.500551
PRIMER_LEFT_1_SEQUENCE=TCTTGTTCTCCTGTCTTCTCCC
PRIMER_RIGHT_1_SEQUENCE=CAAAGAATCAGGGTTTGGAGTT
PRIMER_LEFT_1=36,22
PRIMER_RIGHT_1=137,22
PRIMER_LEFT_1_TM=59.856
PRIMER_RIGHT_1_TM=59.499
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=1.7111
PRIMER_LEFT_2_PENALTY=0.143960
PRIMER_RIGHT_2_PENALTY=1.567125
PRIMER_LEFT_2_SEQUENCE=TCTTGTTCTCCTGTCTTCTCCC
PRIMER_RIGHT_2_SEQUENCE=CCAAAGAATCAGGGTTTGGAGT
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=138,22
PRIMER_LEFT_2_TM=59.856
PRIMER_RIGHT_2_TM=61.567
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=1.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=1.9750
PRIMER_LEFT_3_PENALTY=0.143960
PRIMER_RIGHT_3_PENALTY=1.831057
PRIMER_LEFT_3_SEQUENCE=TCTTGTTCTCCTGTCTTCTCCC
PRIMER_RIGHT_3_SEQUENCE=CCAAAGAATCAGGGTTTGGAG
PRIMER_LEFT_3=36,22
PRIMER_RIGHT_3=138,21
PRIMER_LEFT_3_TM=59.856
PRIMER_RIGHT_3_TM=60.831
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=2.0071
PRIMER_LEFT_4_PENALTY=1.506526
PRIMER_RIGHT_4_PENALTY=0.500551
PRIMER_LEFT_4_SEQUENCE=TTGTTCTCCTGTCTTCTCCCCT
PRIMER_RIGHT_4_SEQUENCE=CAAAGAATCAGGGTTTGGAGTT
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=137,22
PRIMER_LEFT_4_TM=61.507
PRIMER_RIGHT_4_TM=59.499
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=100
=
PRIMER_SEQUENCE_ID=SSR_00706 Ps_contig_mira-and-tgicl-ass_franssen_6839
SEQUENCE=TGCTTCTTTGTCAGAAAAGAAARTACGAGGAAGCAGGTCTCTTAGGCCTGTTTGGATCAGACTCCTTATTTTGCTTCTTCTTCTGACGATTCAATTTTGTTTCCTTTATCATGTTTTTAGTTTTCTCCTTCTTCTTCAGCATTTGCAAGGCTTCATGTTTCTGAAGTTTCATATGCTCTTCCTCATCTTTCATCATATTTGCAGCCTCCTCAGCTTTTTTCTGTTTGTATGCCTCCATTTCTTGTGCATACTTCTCCTTGTTCTTCTTTGCAATCTCTTCATAAGGCCTCTTTTGTTCTTCTGTCATGTTTTTCCATTCCTCACCTGTGATCTTTGCAACCTCCAAGACACCCTTGTTTTCTGCAAGAATAGCCGCTCTTCTATCATTTGTAAACAAGAAATAAGCTGACATGGGATGTTTTGGTTTCAATGAATCCTTCTCTTTCTTCTTGTTCTTCTTCTTGTTCTCTTTCTCTGTCTCTTGTTTGAATTGCATATACTGTTCAAGCAATTCCATAGCAGTCTTATGCTTCTGCTCTTCTTCCAAGAGCTTCATTGCCTCAGTCTCACGTTTTTCCTTTGTAATTACCTGCAAATAAGCTTCTTTTTCTGCAAGGTATTTTTCCTCATAAGGCTTCTTCTCTTCTGCACTAACTGTATTTTTCCATTTCGCACCAAGAATGTTCGATATGTGCTTAAACTCGGCCTCGGGATTAGCCTTCTTTACCTCATGCCATTGGTCTTTGACCCATAACGTGTAGGCATGAGTAGGCCTTTTTGTCTCAGAGCATCCATTCTTCTTCTTCTTGTCCTTCTTCTCTTGTTCCTTATCTTTCACCATAGGCAAATTCATGGTAGGTTTGAATTCCTTCAACTT
TARGET=795,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00707 Ps_contig_mira-and-tgicl-ass_franssen_6840
SEQUENCE=GTTCGATATGTGCTTAAACTCGGCCTCGGGATTAGCCTTCTTTACCTCATGCCATTGGTCTTTGACCCACAACGTGTAAGCATGAGTAGGCCTTTTTGTCTCAGAGCATCCGTTCTTCTTCTTCTTCTTATCCTTCTTTTCTTGTTCCTTATCTTTCACCATAGGCAAACTCATGGTAGGTTTGAATTCCTTCAACTTCTGCAATTTCTTAAGTTCAATCTGAAGTTTCTCATGCTCTTTTCCACGATTTTCGAGTTCTTCATCCTTTTGCTTCAGGATCTCGTCTTTGGCTTTCAAGAGTTCGTCCGTCTTCTCCTTCTCAAGACGCAACTGCTGCAGTTTCTCCTGCATCTCCAGCAAGTCTTGCTCGAACGATTGTTGCTTTGGCTGTTGTTGTTTCTTTGATGCAGGTTTTCCCTTTGATTCCCCAGCTATGATATTGGCATCACTGGCGGATGGGTTTTTCTCCTTGAGAGCCTTTCTGGATCCTCTTGATTTCTTCGTCGGAATTTCAGCAACTGTAGAATCCGCCATTGTTAGGGGGAGAGAGAAAATTGAACAGAGAAATTTCTGAATACTACAAAAATGTAAGAGAATGGTTTGTGCCTCCCCGAA
TARGET=113,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0697
PRIMER_LEFT_PENALTY=0.064105
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=GTCTTTGACCCACAACGTGTAA
PRIMER_RIGHT_SEQUENCE=GTCTTGAGAAGGAGAAGACGGA
PRIMER_LEFT=57,22
PRIMER_RIGHT=325,22
PRIMER_LEFT_TM=59.936
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=269
PRIMER_PAIR_PENALTY_1=0.0697
PRIMER_LEFT_1_PENALTY=0.064105
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=GTCTTTGACCCACAACGTGTAA
PRIMER_RIGHT_1_SEQUENCE=TCTTGAGAAGGAGAAGACGGAC
PRIMER_LEFT_1=57,22
PRIMER_RIGHT_1=324,22
PRIMER_LEFT_1_TM=59.936
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.0724
PRIMER_LEFT_2_PENALTY=0.066849
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=TCTTTGACCCACAACGTGTAAG
PRIMER_RIGHT_2_SEQUENCE=GTCTTGAGAAGGAGAAGACGGA
PRIMER_LEFT_2=58,22
PRIMER_RIGHT_2=325,22
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.0724
PRIMER_LEFT_3_PENALTY=0.066849
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=TCTTTGACCCACAACGTGTAAG
PRIMER_RIGHT_3_SEQUENCE=TCTTGAGAAGGAGAAGACGGAC
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=324,22
PRIMER_LEFT_3_TM=60.067
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.0829
PRIMER_LEFT_4_PENALTY=0.064105
PRIMER_RIGHT_4_PENALTY=0.018835
PRIMER_LEFT_4_SEQUENCE=GTCTTTGACCCACAACGTGTAA
PRIMER_RIGHT_4_SEQUENCE=AGACGAGATCCTGAAGCAAAAG
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=285,22
PRIMER_LEFT_4_TM=59.936
PRIMER_RIGHT_4_TM=60.019
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_00708 Ps_contig_mira-and-tgicl-ass_franssen_6863
SEQUENCE=TATTGTGATAGACAAAAGCTCTAGGTCTTTTTTCCCATGATACAACTTCAACCCAGCGCTCTCCCTTACCATCATCATCATCAACACTATCGACGGTGTTATGAGCAATGGAGGTGAGATTCGGTTT
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00709 Ps_contig_mira-and-tgicl-ass_franssen_6879
SEQUENCE=AAGTAAAAAATGGATCAAAATGTATTGGTAACTTATTCACGGGGCCGATAAGGGTATCCCGTCAATGTATCACACTAGTAAATCAACAAAATCCTGGGATACAATATGCTAAATTCAAACCAAAACGGTTGAAGGAACAAACAACTTCTGAGTAGAACGAACAACTTTGCAGGACATTACATGCAAAAAGCTACCCGAAAAGAAAAGAAAAGTTTTTCATGTTTTTTATATTGCTTCCTCATGCATGATACACTTTCCCTATATCATCCCGCAATAAGATTTTACATCTCACTCAAATCCCAAAAACCCGAAACCTAAATAATAATAATAACTCAAAAAACTCAAGCCGTAACATTCCCTTGTAACCTCTATCTCTATATCTGAATCCTCAAAACAGGCACTTCAGTTAGCTCTAGATAAACCAAACATCTTATGTCTTAAAAACCGAAATATAGTAACAACAGTGGTGTGAGAAAATCACAATTCACCTGCTTCACTGGATTATCTTCGCCTACATGTTCGTGTCATCCTATGTAGTAATTCCCCTATTTGGCTGCCTTTTATTAACAGTATAACCACCAACATAACCAGGAGAAGTCTTGACAGAAACATCTGAAGCTGCACCACCGAGTCCGTAACCGAAGGGACCAGATCCATCTCGTTCAGAATTTGCAGATCGCCAAGTGGAGTCCCCATAGACCGAACTATTGCTGTAAAAATCACCGAAAGAACCATCAACACCACCACCACCATTTGATGTAGAGTATGAAGATGTTGGTGCAAGAGAGGAACCGCTGCTTCTTCCATATCCTTCAGTCCCCAATCCGTAACCGTTGTTGCCACCTCCGTATCCGAGGTTGCCACTACCCGGTGACACATTGTTCCCTCCCCCGTGTCCAGAAATCGGCGAAGAACTCCAGTTGACTCCAGTATTGCCAAATGCATTTCCTCCAACATTTCCACTTCCCGATCCAATGTATGCATTGGAATTCGCAGAATTTGTCCCATAGTTAAGGCTCCCATTCCCCCACAAATTTCGAGTAACAGAACTGAAGAAAGAATTGTTTCCTCCGTTACCACTTTCATATCCAACAGGATTGCCAAACCTATTGGAGCTGCCGATAAAGTACGGATTCAAGCCCCGTCCATAGCTAAGATTGCCATTGAAATTTGCATTTCCTCTAAATCCAGGACTCAAACCCGGCTCGAAATTCATACCCATTCCATAACCAGATCCAAATGGTGCAAATGCATTTCTAGCACTAGCAACCGGACTGAACCTACCATCCATCTGGAGTCCATAGCCTCCGACAGCACTCGGACTATACCCCTGATTGAAGCCATTCAAGAAACTATTAACCCTACTCAGACCATAGTTGTATCCACCGAGAGGTGAGCGGGTAGGTCCTGGCGATAACTCCTTAGGAACTGCTCGCTTGACCTCAACCATTTTACCGTTCAATTCATGAAACGTCTTAAGTAAGACCTGGTCAACAGCTTCTTCGGAATCATACGTGATAAATCCAAATCCTCTTGGCCTCTGAGTGTTGTGATCGTACATTACTACAACATCTGTTATAGTCCCAAACTGATCAAAGTATTTCTTGAAATCACTCTCTGTCACTGTAGAAGCTAAACCTCCAACAAATATCTTTCTCGTGCGACCTTGACCGGGAGAACCATGGATGCTGCCACTGGTTCTACTCAATACGTTTTGATCATCTCTAGGAACAGCTTTCTTCGCCTCAACCATCCTTCCGTCGATGTTGTGCTTCTCCTGGATAACAATCTCTGCAACAGCCGGATCAATGAAAACAACAAAACCAAATCCACGTGCTCTACCAGTTGTCCGATCCTTCATGATCACGGCTTCCTTAACCTCCCCATAAGTACTAAAATACTCCCTGAGTCGCTCTTCGTTGGTGTCCCATGATATTCCACCGATAAATAACTTGCCACTATCTGATTGCATTTTATCTTTGATTATGACAAACTCAGAAACTGATGCAATTATAGATATTGTCGGATTCAATTAAGCACAATCAGATCCTTGATAAACAACAAACAATGAATCCAAGCAAGAACAAAAACAATCCGATTGAAGACGACCCAGATAAGAAAAT
TARGET=197,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1583
PRIMER_LEFT_PENALTY=0.056850
PRIMER_RIGHT_PENALTY=0.101479
PRIMER_LEFT_SEQUENCE=ACGGTTGAAGGAACAAACAACT
PRIMER_RIGHT_SEQUENCE=TTACAAGGGAATGTTACGGCTT
PRIMER_LEFT=124,22
PRIMER_RIGHT=364,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=59.899
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=0.1734
PRIMER_LEFT_1_PENALTY=0.056850
PRIMER_RIGHT_1_PENALTY=0.116582
PRIMER_LEFT_1_SEQUENCE=ACGGTTGAAGGAACAAACAACT
PRIMER_RIGHT_1_SEQUENCE=GAAGTGCCTGTTTTGAGGATTC
PRIMER_LEFT_1=124,22
PRIMER_RIGHT_1=403,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=60.117
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.1818
PRIMER_LEFT_2_PENALTY=0.080323
PRIMER_RIGHT_2_PENALTY=0.101479
PRIMER_LEFT_2_SEQUENCE=TAGAACGAACAACTTTGCAGGA
PRIMER_RIGHT_2_SEQUENCE=TTACAAGGGAATGTTACGGCTT
PRIMER_LEFT_2=152,22
PRIMER_RIGHT_2=364,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=59.899
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=213
PRIMER_PAIR_PENALTY_3=0.1969
PRIMER_LEFT_3_PENALTY=0.080323
PRIMER_RIGHT_3_PENALTY=0.116582
PRIMER_LEFT_3_SEQUENCE=TAGAACGAACAACTTTGCAGGA
PRIMER_RIGHT_3_SEQUENCE=GAAGTGCCTGTTTTGAGGATTC
PRIMER_LEFT_3=152,22
PRIMER_RIGHT_3=403,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=60.117
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.2670
PRIMER_LEFT_4_PENALTY=0.165541
PRIMER_RIGHT_4_PENALTY=0.101479
PRIMER_LEFT_4_SEQUENCE=CGAACAACTTTGCAGGACATTA
PRIMER_RIGHT_4_SEQUENCE=TTACAAGGGAATGTTACGGCTT
PRIMER_LEFT_4=157,22
PRIMER_RIGHT_4=364,22
PRIMER_LEFT_4_TM=60.166
PRIMER_RIGHT_4_TM=59.899
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_00710 Ps_contig_mira-and-tgicl-ass_franssen_6879
SEQUENCE=AAGTAAAAAATGGATCAAAATGTATTGGTAACTTATTCACGGGGCCGATAAGGGTATCCCGTCAATGTATCACACTAGTAAATCAACAAAATCCTGGGATACAATATGCTAAATTCAAACCAAAACGGTTGAAGGAACAAACAACTTCTGAGTAGAACGAACAACTTTGCAGGACATTACATGCAAAAAGCTACCCGAAAAGAAAAGAAAAGTTTTTCATGTTTTTTATATTGCTTCCTCATGCATGATACACTTTCCCTATATCATCCCGCAATAAGATTTTACATCTCACTCAAATCCCAAAAACCCGAAACCTAAATAATAATAATAACTCAAAAAACTCAAGCCGTAACATTCCCTTGTAACCTCTATCTCTATATCTGAATCCTCAAAACAGGCACTTCAGTTAGCTCTAGATAAACCAAACATCTTATGTCTTAAAAACCGAAATATAGTAACAACAGTGGTGTGAGAAAATCACAATTCACCTGCTTCACTGGATTATCTTCGCCTACATGTTCGTGTCATCCTATGTAGTAATTCCCCTATTTGGCTGCCTTTTATTAACAGTATAACCACCAACATAACCAGGAGAAGTCTTGACAGAAACATCTGAAGCTGCACCACCGAGTCCGTAACCGAAGGGACCAGATCCATCTCGTTCAGAATTTGCAGATCGCCAAGTGGAGTCCCCATAGACCGAACTATTGCTGTAAAAATCACCGAAAGAACCATCAACACCACCACCACCATTTGATGTAGAGTATGAAGATGTTGGTGCAAGAGAGGAACCGCTGCTTCTTCCATATCCTTCAGTCCCCAATCCGTAACCGTTGTTGCCACCTCCGTATCCGAGGTTGCCACTACCCGGTGACACATTGTTCCCTCCCCCGTGTCCAGAAATCGGCGAAGAACTCCAGTTGACTCCAGTATTGCCAAATGCATTTCCTCCAACATTTCCACTTCCCGATCCAATGTATGCATTGGAATTCGCAGAATTTGTCCCATAGTTAAGGCTCCCATTCCCCCACAAATTTCGAGTAACAGAACTGAAGAAAGAATTGTTTCCTCCGTTACCACTTTCATATCCAACAGGATTGCCAAACCTATTGGAGCTGCCGATAAAGTACGGATTCAAGCCCCGTCCATAGCTAAGATTGCCATTGAAATTTGCATTTCCTCTAAATCCAGGACTCAAACCCGGCTCGAAATTCATACCCATTCCATAACCAGATCCAAATGGTGCAAATGCATTTCTAGCACTAGCAACCGGACTGAACCTACCATCCATCTGGAGTCCATAGCCTCCGACAGCACTCGGACTATACCCCTGATTGAAGCCATTCAAGAAACTATTAACCCTACTCAGACCATAGTTGTATCCACCGAGAGGTGAGCGGGTAGGTCCTGGCGATAACTCCTTAGGAACTGCTCGCTTGACCTCAACCATTTTACCGTTCAATTCATGAAACGTCTTAAGTAAGACCTGGTCAACAGCTTCTTCGGAATCATACGTGATAAATCCAAATCCTCTTGGCCTCTGAGTGTTGTGATCGTACATTACTACAACATCTGTTATAGTCCCAAACTGATCAAAGTATTTCTTGAAATCACTCTCTGTCACTGTAGAAGCTAAACCTCCAACAAATATCTTTCTCGTGCGACCTTGACCGGGAGAACCATGGATGCTGCCACTGGTTCTACTCAATACGTTTTGATCATCTCTAGGAACAGCTTTCTTCGCCTCAACCATCCTTCCGTCGATGTTGTGCTTCTCCTGGATAACAATCTCTGCAACAGCCGGATCAATGAAAACAACAAAACCAAATCCACGTGCTCTACCAGTTGTCCGATCCTTCATGATCACGGCTTCCTTAACCTCCCCATAAGTACTAAAATACTCCCTGAGTCGCTCTTCGTTGGTGTCCCATGATATTCCACCGATAAATAACTTGCCACTATCTGATTGCATTTTATCTTTGATTATGACAAACTCAGAAACTGATGCAATTATAGATATTGTCGGATTCAATTAAGCACAATCAGATCCTTGATAAACAACAAACAATGAATCCAAGCAAGAACAAAAACAATCCGATTGAAGACGACCCAGATAAGAAAAT
TARGET=318,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1583
PRIMER_LEFT_PENALTY=0.056850
PRIMER_RIGHT_PENALTY=0.101479
PRIMER_LEFT_SEQUENCE=ACGGTTGAAGGAACAAACAACT
PRIMER_RIGHT_SEQUENCE=TTACAAGGGAATGTTACGGCTT
PRIMER_LEFT=124,22
PRIMER_RIGHT=364,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=59.899
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=0.1734
PRIMER_LEFT_1_PENALTY=0.056850
PRIMER_RIGHT_1_PENALTY=0.116582
PRIMER_LEFT_1_SEQUENCE=ACGGTTGAAGGAACAAACAACT
PRIMER_RIGHT_1_SEQUENCE=GAAGTGCCTGTTTTGAGGATTC
PRIMER_LEFT_1=124,22
PRIMER_RIGHT_1=403,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=60.117
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.1818
PRIMER_LEFT_2_PENALTY=0.080323
PRIMER_RIGHT_2_PENALTY=0.101479
PRIMER_LEFT_2_SEQUENCE=TAGAACGAACAACTTTGCAGGA
PRIMER_RIGHT_2_SEQUENCE=TTACAAGGGAATGTTACGGCTT
PRIMER_LEFT_2=152,22
PRIMER_RIGHT_2=364,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=59.899
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=213
PRIMER_PAIR_PENALTY_3=0.1969
PRIMER_LEFT_3_PENALTY=0.080323
PRIMER_RIGHT_3_PENALTY=0.116582
PRIMER_LEFT_3_SEQUENCE=TAGAACGAACAACTTTGCAGGA
PRIMER_RIGHT_3_SEQUENCE=GAAGTGCCTGTTTTGAGGATTC
PRIMER_LEFT_3=152,22
PRIMER_RIGHT_3=403,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=60.117
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.2670
PRIMER_LEFT_4_PENALTY=0.165541
PRIMER_RIGHT_4_PENALTY=0.101479
PRIMER_LEFT_4_SEQUENCE=CGAACAACTTTGCAGGACATTA
PRIMER_RIGHT_4_SEQUENCE=TTACAAGGGAATGTTACGGCTT
PRIMER_LEFT_4=157,22
PRIMER_RIGHT_4=364,22
PRIMER_LEFT_4_TM=60.166
PRIMER_RIGHT_4_TM=59.899
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_00711 Ps_contig_mira-and-tgicl-ass_franssen_6879
SEQUENCE=AAGTAAAAAATGGATCAAAATGTATTGGTAACTTATTCACGGGGCCGATAAGGGTATCCCGTCAATGTATCACACTAGTAAATCAACAAAATCCTGGGATACAATATGCTAAATTCAAACCAAAACGGTTGAAGGAACAAACAACTTCTGAGTAGAACGAACAACTTTGCAGGACATTACATGCAAAAAGCTACCCGAAAAGAAAAGAAAAGTTTTTCATGTTTTTTATATTGCTTCCTCATGCATGATACACTTTCCCTATATCATCCCGCAATAAGATTTTACATCTCACTCAAATCCCAAAAACCCGAAACCTAAATAATAATAATAACTCAAAAAACTCAAGCCGTAACATTCCCTTGTAACCTCTATCTCTATATCTGAATCCTCAAAACAGGCACTTCAGTTAGCTCTAGATAAACCAAACATCTTATGTCTTAAAAACCGAAATATAGTAACAACAGTGGTGTGAGAAAATCACAATTCACCTGCTTCACTGGATTATCTTCGCCTACATGTTCGTGTCATCCTATGTAGTAATTCCCCTATTTGGCTGCCTTTTATTAACAGTATAACCACCAACATAACCAGGAGAAGTCTTGACAGAAACATCTGAAGCTGCACCACCGAGTCCGTAACCGAAGGGACCAGATCCATCTCGTTCAGAATTTGCAGATCGCCAAGTGGAGTCCCCATAGACCGAACTATTGCTGTAAAAATCACCGAAAGAACCATCAACACCACCACCACCATTTGATGTAGAGTATGAAGATGTTGGTGCAAGAGAGGAACCGCTGCTTCTTCCATATCCTTCAGTCCCCAATCCGTAACCGTTGTTGCCACCTCCGTATCCGAGGTTGCCACTACCCGGTGACACATTGTTCCCTCCCCCGTGTCCAGAAATCGGCGAAGAACTCCAGTTGACTCCAGTATTGCCAAATGCATTTCCTCCAACATTTCCACTTCCCGATCCAATGTATGCATTGGAATTCGCAGAATTTGTCCCATAGTTAAGGCTCCCATTCCCCCACAAATTTCGAGTAACAGAACTGAAGAAAGAATTGTTTCCTCCGTTACCACTTTCATATCCAACAGGATTGCCAAACCTATTGGAGCTGCCGATAAAGTACGGATTCAAGCCCCGTCCATAGCTAAGATTGCCATTGAAATTTGCATTTCCTCTAAATCCAGGACTCAAACCCGGCTCGAAATTCATACCCATTCCATAACCAGATCCAAATGGTGCAAATGCATTTCTAGCACTAGCAACCGGACTGAACCTACCATCCATCTGGAGTCCATAGCCTCCGACAGCACTCGGACTATACCCCTGATTGAAGCCATTCAAGAAACTATTAACCCTACTCAGACCATAGTTGTATCCACCGAGAGGTGAGCGGGTAGGTCCTGGCGATAACTCCTTAGGAACTGCTCGCTTGACCTCAACCATTTTACCGTTCAATTCATGAAACGTCTTAAGTAAGACCTGGTCAACAGCTTCTTCGGAATCATACGTGATAAATCCAAATCCTCTTGGCCTCTGAGTGTTGTGATCGTACATTACTACAACATCTGTTATAGTCCCAAACTGATCAAAGTATTTCTTGAAATCACTCTCTGTCACTGTAGAAGCTAAACCTCCAACAAATATCTTTCTCGTGCGACCTTGACCGGGAGAACCATGGATGCTGCCACTGGTTCTACTCAATACGTTTTGATCATCTCTAGGAACAGCTTTCTTCGCCTCAACCATCCTTCCGTCGATGTTGTGCTTCTCCTGGATAACAATCTCTGCAACAGCCGGATCAATGAAAACAACAAAACCAAATCCACGTGCTCTACCAGTTGTCCGATCCTTCATGATCACGGCTTCCTTAACCTCCCCATAAGTACTAAAATACTCCCTGAGTCGCTCTTCGTTGGTGTCCCATGATATTCCACCGATAAATAACTTGCCACTATCTGATTGCATTTTATCTTTGATTATGACAAACTCAGAAACTGATGCAATTATAGATATTGTCGGATTCAATTAAGCACAATCAGATCCTTGATAAACAACAAACAATGAATCCAAGCAAGAACAAAAACAATCCGATTGAAGACGACCCAGATAAGAAAAT
TARGET=739,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2321
PRIMER_LEFT_PENALTY=0.228464
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=AATCACCGAAAGAACCATCAAC
PRIMER_RIGHT_SEQUENCE=CATTTGGCAATACTGGAGTCAA
PRIMER_LEFT=717,22
PRIMER_RIGHT=941,22
PRIMER_LEFT_TM=60.228
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.3966
PRIMER_LEFT_1_PENALTY=0.228464
PRIMER_RIGHT_1_PENALTY=0.168114
PRIMER_LEFT_1_SEQUENCE=AATCACCGAAAGAACCATCAAC
PRIMER_RIGHT_1_SEQUENCE=TTGGCAATACTGGAGTCAACTG
PRIMER_LEFT_1=717,22
PRIMER_RIGHT_1=938,22
PRIMER_LEFT_1_TM=60.228
PRIMER_RIGHT_1_TM=60.168
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.4495
PRIMER_LEFT_2_PENALTY=0.228464
PRIMER_RIGHT_2_PENALTY=0.220992
PRIMER_LEFT_2_SEQUENCE=AATCACCGAAAGAACCATCAAC
PRIMER_RIGHT_2_SEQUENCE=AGTGGAAATGTTGGAGGAAATG
PRIMER_LEFT_2=717,22
PRIMER_RIGHT_2=963,22
PRIMER_LEFT_2_TM=60.228
PRIMER_RIGHT_2_TM=60.221
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.4631
PRIMER_LEFT_3_PENALTY=0.459539
PRIMER_RIGHT_3_PENALTY=0.003589
PRIMER_LEFT_3_SEQUENCE=ATCCATCTCGTTCAGAATTTGC
PRIMER_RIGHT_3_SEQUENCE=CATTTGGCAATACTGGAGTCAA
PRIMER_LEFT_3=651,22
PRIMER_RIGHT_3=941,22
PRIMER_LEFT_3_TM=60.460
PRIMER_RIGHT_3_TM=59.996
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.4673
PRIMER_LEFT_4_PENALTY=0.463680
PRIMER_RIGHT_4_PENALTY=0.003589
PRIMER_LEFT_4_SEQUENCE=CCAGATCCATCTCGTTCAGAAT
PRIMER_RIGHT_4_SEQUENCE=CATTTGGCAATACTGGAGTCAA
PRIMER_LEFT_4=647,22
PRIMER_RIGHT_4=941,22
PRIMER_LEFT_4_TM=60.464
PRIMER_RIGHT_4_TM=59.996
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_00712 Ps_contig_mira-and-tgicl-ass_franssen_6906
SEQUENCE=TACATCACTGTTATCTACAACATGGAAGATTTTCTTGGCGATTCTGATCAAAATAGAACAAACAAGGGTCTTCAGCCCTCCAAACAAGCTGTCACCGTTGCTTGAATTTCAGATGTCCTGAAATGGTGGGAGCAATAGATTTTATCAAGACTTTGATATATCTTAGCAGTGAACATTCTTTTTTTTGCAATAGGAATTGAATTTCTGGTATTCTGGCAGATCAAAGTAGAAATGGTTAATAGGAAAGAGTTTATGGTAAGTACAACAAAATTGTTAGTTAGTTAACTGTGGTCAAGAATTTTGCTATTGAACTCAATAGTTATTTGATATGATACTATGTTAAATTATGAAACAAATTGATCTGATAGACTTCATAATAGTTCATCTTTACAAATTTATAGAAGCTACTATGATATTTTTTCCCCCTTTTGG
TARGET=273,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00713 Ps_contig_mira-and-tgicl-ass_franssen_6913
SEQUENCE=AAACATTTTCTTATCGATTCTCAGCAACAACATTTTTTTTGTTACCTGAGGTAAAGGGAGTTGCACAACAATCCCATGCACATCATCATCATCATTGAAACCAGAAACAACATCAAGCAGTTCATTTTCRGTACAACTCTCTGGCAACTCAGAAACCAACARTTCCAATCCAACTTTATCACAAGCTTTCATACTTTAACAATGAATAGAAAGTATGAGATCTTCTNTCACCTACCAAACCACAGCATA
TARGET=81,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00714 Ps_contig_mira-and-tgicl-ass_franssen_6934
SEQUENCE=TTTCTTCGTTGGGTCGTACCACGTTTAAACTTTTAGCTTTTCTTTTATTNTTGTTTCTAGGACAGGAAGGACGATTACTTTGTCTAAAACCTTACGGTCTAAGTTTGTTAAATAGTTCCCTTTCGTTTATTTGTCAGATGCMACTACACACCACACCATCATCATATTCAACCTCAAACAAACACTTACCACGAATCTATGCTTTTTTTTCTTGCCTAAAAATAATCCCAATTTATAACATAAAATCAAACACCCAACAATCACAAATCAAATCAACCTTGTTCCATTCCATCCATGGCTGCTGCTGCTGCGTTTCGATCGAAGCCTTCAAACTACAAAGATAAAGCAACCCTTATGTTATCTCAGTTAAGGTACTTCGCTTTCAACTACACTAACGTTGGTAATTCTCACTCTCCACCTCGCTCTCCGTTAATCAACACCCCTTATCACTTCACTTCCTTCAAACCCCTTTCCCTCCGCGGCGATTTCACTCACAACAATCCAACGGCTCGTAGTAACACCGCTCATTTCGATGACTCTACCACACCCAATCAAAACCCTAATCTCGTTCCTTATGATAGAACCGCGTATAGGGTTCTTGTTTCTTCATTTGGGGATCCTCCTGAAGTTTGGTCTGGCGCTGGAATTGTTGTTCGCCCGGGGAATTCCGGTTATGATGTGTCTGGTGGCGGTGGTGGTGGGGGTGGAGGTGGTGCTAGCTCCGGTGGTGACTCCGGGTCGGTGAATTCGAAAGATGGATGTTGGGGTGGGTCTAATTTGGGAGGTAGTTTCCCTACTCCGAAGGAAATTTGCAAGGGGCTTGATAAATTCGTAATTGGACAAGAGAGAGCTAAGAAGGTGCTTTCTGTGGCAGTTTATAATCACTATAAGAGAATATTTCATGAAAAGTCCATGTGGCCTGCAGGAGATTCAAGTAATGATGGTAAAGCCAATGTTGCTGTTGATGATGATGATGAAGAAGTTGAACTTGAGAAAAGTAATATCCTTTTGATGGGGCCGACGGGATCAGGGAAGACATTACTTGCTAAAACCTTGGCTAAGTTTGTAAATGTTCCCTTTGTTATTGCAGATGCAACTACACTCACTCAGGCTGGTTATGTTGGAGAAGATGTGGAGTCCATATTATACAAGCTCCTCATGGCTGCAGATTATAACGTGGCAGCTGCTCAGCAAGGCATTGTTTACATTGATGAAGTTGACAAGATTAACCAAAAGTCTGAGAGACTTAATAT
TARGET=298,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00715 Ps_contig_mira-and-tgicl-ass_franssen_6934
SEQUENCE=TTTCTTCGTTGGGTCGTACCACGTTTAAACTTTTAGCTTTTCTTTTATTNTTGTTTCTAGGACAGGAAGGACGATTACTTTGTCTAAAACCTTACGGTCTAAGTTTGTTAAATAGTTCCCTTTCGTTTATTTGTCAGATGCMACTACACACCACACCATCATCATATTCAACCTCAAACAAACACTTACCACGAATCTATGCTTTTTTTTCTTGCCTAAAAATAATCCCAATTTATAACATAAAATCAAACACCCAACAATCACAAATCAAATCAACCTTGTTCCATTCCATCCATGGCTGCTGCTGCTGCGTTTCGATCGAAGCCTTCAAACTACAAAGATAAAGCAACCCTTATGTTATCTCAGTTAAGGTACTTCGCTTTCAACTACACTAACGTTGGTAATTCTCACTCTCCACCTCGCTCTCCGTTAATCAACACCCCTTATCACTTCACTTCCTTCAAACCCCTTTCCCTCCGCGGCGATTTCACTCACAACAATCCAACGGCTCGTAGTAACACCGCTCATTTCGATGACTCTACCACACCCAATCAAAACCCTAATCTCGTTCCTTATGATAGAACCGCGTATAGGGTTCTTGTTTCTTCATTTGGGGATCCTCCTGAAGTTTGGTCTGGCGCTGGAATTGTTGTTCGCCCGGGGAATTCCGGTTATGATGTGTCTGGTGGCGGTGGTGGTGGGGGTGGAGGTGGTGCTAGCTCCGGTGGTGACTCCGGGTCGGTGAATTCGAAAGATGGATGTTGGGGTGGGTCTAATTTGGGAGGTAGTTTCCCTACTCCGAAGGAAATTTGCAAGGGGCTTGATAAATTCGTAATTGGACAAGAGAGAGCTAAGAAGGTGCTTTCTGTGGCAGTTTATAATCACTATAAGAGAATATTTCATGAAAAGTCCATGTGGCCTGCAGGAGATTCAAGTAATGATGGTAAAGCCAATGTTGCTGTTGATGATGATGATGAAGAAGTTGAACTTGAGAAAAGTAATATCCTTTTGATGGGGCCGACGGGATCAGGGAAGACATTACTTGCTAAAACCTTGGCTAAGTTTGTAAATGTTCCCTTTGTTATTGCAGATGCAACTACACTCACTCAGGCTGGTTATGTTGGAGAAGATGTGGAGTCCATATTATACAAGCTCCTCATGGCTGCAGATTATAACGTGGCAGCTGCTCAGCAAGGCATTGTTTACATTGATGAAGTTGACAAGATTAACCAAAAGTCTGAGAGACTTAATAT
TARGET=963,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00716 Ps_contig_mira-and-tgicl-ass_franssen_6946
SEQUENCE=TAGRTGCAAAATGCAATTGTATAATGATGCAAAATAAGTACAACAGTTTGTTACTGTGACATCATTAAACACCTAACTATTTTCATGTTGATATCTTTCAAGCATCCAGAACTCCACCAAAACTAGAAACATAATCTAACCAAAAAACACTTGAAAGATTTGAGAACAAAATGAAGAAGAAGAAAAAAAGAAATTACTAAATCTTAGGGTGTGTTACATTCTAATAAATGAAGGGAAATAGTTAG
TARGET=173,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00717 Ps_contig_mira-and-tgicl-ass_franssen_6952
SEQUENCE=CATTGAAACTGAAACAGCTCAAAGTTCTTTGTTGATCAACATTCATCTTCGGTTTTGTCCGAGTATTCTTCTAAAAAAGAGGCTATTGCTTACTTGAAGCAAAAGCTCGCAGGTAGTAAGAATTTTTGTATTGAAGGGAAGGCAGTAAGATTGGCATCGAAGACAAGTTAAAATTTTGATACATTTTTGGCGGCTTTTCTTATTCTTTTCATGCTGGGGAGAAAAAAGGGTTAAGGTGTTCCTAGATTGCATTGGTTGGAGTTAGTATAGTATAGGCAAATGTTGTAATAATATGTTAATGGTTACAAATTTACAATAGCTTTTTGTTAAATTGAATTTATAATTATTTTTAAGTGTTAGTTTAAAATGTTGCAAGTGTGTGTGTAATGGTATTCATTGGATTATATATTGAAATTATTTTTGTGCTCTAVMA
TARGET=376,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00718 Ps_contig_mira-and-tgicl-ass_franssen_6988
SEQUENCE=TACGGGGGCTTAACGCCTCTCTAATAACCCCCACCCTACCTTTTCCATTTCCGTTTCTCATCTCTTCTCTTCAAATCACACACTCTCTCTACCTTTCTCTCATCAATGGACACTCCTCATTCCCCTTCTTCCTGTTCCTCTTCCTCCTCCTCCTCCTATTCATGGGAAGGACACGCTGCCGACACAATCAGTTTAGAGCCAGAAGAGCCGCAAACGCCGATAGCCGTCCGGCGTGCTCTTCAGCTTTTGAAATCCGGCTTGCCGGAGTTACGTCTTCCAAGCCGCTCGCGACATCCGGAAACTCACGAAAACCTCGCAGCGGTGTCGGCGTCAGCTTTCTGAATCTGTCGGTCCACTCGTTTCTATGCTCCGAGTCGACTCGGCCGAGTCACATGAACCAGCGCTTCTCGCTTTGCTTAATCTCGCCGTCAAAGATGAAAAGAATAAAATCAAGATTGTGGAGGCCGGAGCATTAGAACCAATACTTAGTTTCCTCAAGTCACAAAATCCAAATCTTCAGGCATCTGCAACCGCGGGCTTTTGCTCACACTATCTGCTT
TARGET=142,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1858
PRIMER_LEFT_PENALTY=0.034674
PRIMER_RIGHT_PENALTY=0.151106
PRIMER_LEFT_SEQUENCE=CTCTAATAACCCCCACCCTACC
PRIMER_RIGHT_SEQUENCE=GGCTCTTCTGGCTCTAAACTGA
PRIMER_LEFT=18,22
PRIMER_RIGHT=208,22
PRIMER_LEFT_TM=59.965
PRIMER_RIGHT_TM=60.151
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.1858
PRIMER_LEFT_1_PENALTY=0.034674
PRIMER_RIGHT_1_PENALTY=0.151106
PRIMER_LEFT_1_SEQUENCE=TCTAATAACCCCCACCCTACCT
PRIMER_RIGHT_1_SEQUENCE=GGCTCTTCTGGCTCTAAACTGA
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=208,22
PRIMER_LEFT_1_TM=59.965
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.2256
PRIMER_LEFT_2_PENALTY=0.074466
PRIMER_RIGHT_2_PENALTY=0.151106
PRIMER_LEFT_2_SEQUENCE=TCCATTTCCGTTTCTCATCTCT
PRIMER_RIGHT_2_SEQUENCE=GGCTCTTCTGGCTCTAAACTGA
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=208,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=60.151
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=0.2847
PRIMER_LEFT_3_PENALTY=0.197963
PRIMER_RIGHT_3_PENALTY=0.086698
PRIMER_LEFT_3_SEQUENCE=ATCAATGGACACTCCTCATTCC
PRIMER_RIGHT_3_SEQUENCE=AGTCGACTCGGAGCATAGAAAC
PRIMER_LEFT_3=101,22
PRIMER_RIGHT_3=379,22
PRIMER_LEFT_3_TM=60.198
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.3025
PRIMER_LEFT_4_PENALTY=0.151423
PRIMER_RIGHT_4_PENALTY=0.151106
PRIMER_LEFT_4_SEQUENCE=TAATAACCCCCACCCTACCTTT
PRIMER_RIGHT_4_SEQUENCE=GGCTCTTCTGGCTCTAAACTGA
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=208,22
PRIMER_LEFT_4_TM=59.849
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_00719 Ps_contig_mira-and-tgicl-ass_franssen_6990
SEQUENCE=CACTCGACGCAAGCCAGCAAGGAAATAGCTGTTGCAATAMACCACGCATGACAGTATGCTGCAGGATATGCTTGGCGGCCGGATTCCCAACCTGCATCAACCTGCAGAACTTATTTTTCACGGGGCAGACTTCACTATGAAATGGAATTCACAACCGTTTCTTCCCTTTCATTCCAATCTCCATTGTTCTTCACTCCAATCTTCTTCTTCTACGGCGGGAATCTTCTTCATACCAAGAGTCTCTAATCTTCTGTCATC
TARGET=200,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00720 Ps_contig_mira-and-tgicl-ass_franssen_6994
SEQUENCE=CGTAAGAAGTTAGTCAAAAAGAACTAGGTGAGAAGCATGTTCCTGTGGAGTTAGCCTTGTATATTGCCAAAGATATCTCATGCGCTTTGTCGGAACTGCATTCAAAGCACATAATTCACCGTGACATAAAAAGCGAAAACATTCTGTTTGATTGTGATAGGAAGAAAGATGATGGAACTCCCACTGTGAAGCTCTGTGATTTTGATAGTGCAGTGCCACTAAGATCACCTTTACATGCATGCTGTATTGCTCATGTAGGATCACCTCCTCCTTGTGTGTGTGTTGGAACACCTCGTTGGATGGCTCCAGAGGTCATGCGGACTATGTACGAAAAAACACTTATGGA
TARGET=273,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1622
PRIMER_LEFT_PENALTY=0.137575
PRIMER_RIGHT_PENALTY=0.024665
PRIMER_LEFT_SEQUENCE=AAGATGATGGAACTCCCACTGT
PRIMER_RIGHT_SEQUENCE=TTTCGTACATAGTCCGCATGAC
PRIMER_LEFT=165,22
PRIMER_RIGHT=332,22
PRIMER_LEFT_TM=59.862
PRIMER_RIGHT_TM=60.025
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.1628
PRIMER_LEFT_1_PENALTY=0.138114
PRIMER_RIGHT_1_PENALTY=0.024665
PRIMER_LEFT_1_SEQUENCE=GGAACTGCATTCAAAGCACATA
PRIMER_RIGHT_1_SEQUENCE=TTTCGTACATAGTCCGCATGAC
PRIMER_LEFT_1=91,22
PRIMER_RIGHT_1=332,22
PRIMER_LEFT_1_TM=60.138
PRIMER_RIGHT_1_TM=60.025
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.2916
PRIMER_LEFT_2_PENALTY=0.137575
PRIMER_RIGHT_2_PENALTY=0.154023
PRIMER_LEFT_2_SEQUENCE=AAGATGATGGAACTCCCACTGT
PRIMER_RIGHT_2_SEQUENCE=TACATAGTCCGCATGACCTCTG
PRIMER_LEFT_2=165,22
PRIMER_RIGHT_2=327,22
PRIMER_LEFT_2_TM=59.862
PRIMER_RIGHT_2_TM=60.154
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=0.2921
PRIMER_LEFT_3_PENALTY=0.138114
PRIMER_RIGHT_3_PENALTY=0.154023
PRIMER_LEFT_3_SEQUENCE=GGAACTGCATTCAAAGCACATA
PRIMER_RIGHT_3_SEQUENCE=TACATAGTCCGCATGACCTCTG
PRIMER_LEFT_3=91,22
PRIMER_RIGHT_3=327,22
PRIMER_LEFT_3_TM=60.138
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=0.3285
PRIMER_LEFT_4_PENALTY=0.174518
PRIMER_RIGHT_4_PENALTY=0.154023
PRIMER_LEFT_4_SEQUENCE=AGAAGCATGTTCCTGTGGAGTT
PRIMER_RIGHT_4_SEQUENCE=TACATAGTCCGCATGACCTCTG
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=327,22
PRIMER_LEFT_4_TM=60.175
PRIMER_RIGHT_4_TM=60.154
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_00721 Ps_contig_mira-and-tgicl-ass_franssen_7006
SEQUENCE=TGATGACAGTGCCTGTTTTGATTCTCACTGTTTCGGCGGCCGCTGTTTTTCTGTTCTTCTTGTTGTCCTCTCTCTCTTCTTGCTCATGCCCTTCACAACCTTCATCCACCCCTGTCAACAATGCAGAGAACTCTGCTGTTGGTTTGTCCGAGTCTAGTAGTGGTAATGGTTTGTTAGCAACAAGGAAGGAGGATGTTCAGTGGGTGAAAGATCAAATACAAGCTAATGGACTTCATATGCAAGAAAATGTGCTGCGCAAGGGTATTAATCCTCGCACTAGGGTTCAACAACTCGAGGATCTTAGACAATTTAAGGGCATAATCACACTATGAGGGACCTGACTCAAAC
TARGET=68,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0749
PRIMER_LEFT_PENALTY=0.072552
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=CCGCTGTTTTTCTGTTCTTCTT
PRIMER_RIGHT_SEQUENCE=ATCCTCGAGTTGTTGAACCCTA
PRIMER_LEFT=39,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=59.927
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.1076
PRIMER_LEFT_1_PENALTY=0.072552
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=CCGCTGTTTTTCTGTTCTTCTT
PRIMER_RIGHT_1_SEQUENCE=CCTTCCTTGTTGCTAACAAACC
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=188,22
PRIMER_LEFT_1_TM=59.927
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=150
PRIMER_PAIR_PENALTY_2=0.2029
PRIMER_LEFT_2_PENALTY=0.072552
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=CCGCTGTTTTTCTGTTCTTCTT
PRIMER_RIGHT_2_SEQUENCE=ACATCCTCCTTCCTTGTTGCTA
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=195,22
PRIMER_LEFT_2_TM=59.927
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.2222
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=CAGTGCCTGTTTTGATTCTCAC
PRIMER_RIGHT_3_SEQUENCE=ATCCTCGAGTTGTTGAACCCTA
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=298,22
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.2222
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=0.002310
PRIMER_LEFT_4_SEQUENCE=GTGCCTGTTTTGATTCTCACTG
PRIMER_RIGHT_4_SEQUENCE=ATCCTCGAGTTGTTGAACCCTA
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=298,22
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=59.998
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_00722 Ps_contig_mira-and-tgicl-ass_franssen_7008
SEQUENCE=CTACACAAAATTAATGACAGCTAACATATGAACCTTTAGTTTAAAGAKTTTGTCAGAATGCAGTTATCCTTATTAAAAGTAAAAAAAAATAACACTTCAAGCAACTACCTTCATCTCTTGATGATGATGAATCAATAATTACTACGTATATAACTTAAAAATAAACACAAATTTAAGTTACATTCTTCTCTATGCATTCATCAAGTCTTTTATTATTGTAAACTTTTGTCCTAGAACTCTATA
TARGET=119,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00723 Ps_contig_mira-and-tgicl-ass_franssen_7019
SEQUENCE=GTCGGTCGGTACTARAAAMBAAAACAAAAAAATCGAAATCGAAATSAAGATTCTAGGGCTTGTGCTGTGACTCCGAGGTGCAATCGAACTTCAATGGACGACATGGCAGCCTACTACGCTCAGAACCAACCGCCGAATCTCCTAACTTATCAGTATTATCCACCGCCGCCGCCTCCTCCTCATCAACCCATGGCGGTGATACCACCGCCCCCTGGTGCTCCACTACCGCCGCCGCCGCACCACCACCACCTTCCGCCACAGTACCACCCGCAAACCCGTCTATGGTTCTTACGTTCCTCCTCTATTCTT
TARGET=226,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00724 Ps_contig_mira-and-tgicl-ass_franssen_7019
SEQUENCE=GTCGGTCGGTACTARAAAMBAAAACAAAAAAATCGAAATCGAAATSAAGATTCTAGGGCTTGTGCTGTGACTCCGAGGTGCAATCGAACTTCAATGGACGACATGGCAGCCTACTACGCTCAGAACCAACCGCCGAATCTCCTAACTTATCAGTATTATCCACCGCCGCCGCCTCCTCCTCATCAACCCATGGCGGTGATACCACCGCCCCCTGGTGCTCCACTACCGCCGCCGCCGCACCACCACCACCTTCCGCCACAGTACCACCCGCAAACCCGTCTATGGTTCTTACGTTCCTCCTCTATTCTT
TARGET=238,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00725 Ps_contig_mira-and-tgicl-ass_franssen_7064
SEQUENCE=GTCGGGGACGTCTCATTTTCGACTCTTATTCAACATCACTGGTTTTGTTTAGGTTTCGTGCTGCTGTTCACACTTTACAACAACAACAACAAAAAATGCAGTCAAGTCCTTTCG
TARGET=77,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8851
PRIMER_LEFT_PENALTY=0.243031
PRIMER_RIGHT_PENALTY=0.642030
PRIMER_LEFT_SEQUENCE=GGACGTCTCATTTTCGACTCTT
PRIMER_RIGHT_SEQUENCE=CGAAAGGACTTGACTGCATTTT
PRIMER_LEFT=5,22
PRIMER_RIGHT=113,22
PRIMER_LEFT_TM=59.757
PRIMER_RIGHT_TM=60.642
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=1.8663
PRIMER_LEFT_1_PENALTY=1.224301
PRIMER_RIGHT_1_PENALTY=0.642030
PRIMER_LEFT_1_SEQUENCE=GGACGTCTCATTTTCGACTCTTA
PRIMER_RIGHT_1_SEQUENCE=CGAAAGGACTTGACTGCATTTT
PRIMER_LEFT_1=5,23
PRIMER_RIGHT_1=113,22
PRIMER_LEFT_1_TM=59.776
PRIMER_RIGHT_1_TM=60.642
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=1.8743
PRIMER_LEFT_2_PENALTY=0.243031
PRIMER_RIGHT_2_PENALTY=1.631316
PRIMER_LEFT_2_SEQUENCE=GGACGTCTCATTTTCGACTCTT
PRIMER_RIGHT_2_SEQUENCE=CGAAAGGACTTGACTGCATTT
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=113,21
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=59.369
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=2.5264
PRIMER_LEFT_3_PENALTY=1.884339
PRIMER_RIGHT_3_PENALTY=0.642030
PRIMER_LEFT_3_SEQUENCE=GGGACGTCTCATTTTCGACTCT
PRIMER_RIGHT_3_SEQUENCE=CGAAAGGACTTGACTGCATTTT
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=113,22
PRIMER_LEFT_3_TM=61.884
PRIMER_RIGHT_3_TM=60.642
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=110
PRIMER_PAIR_PENALTY_4=2.6562
PRIMER_LEFT_4_PENALTY=2.014145
PRIMER_RIGHT_4_PENALTY=0.642030
PRIMER_LEFT_4_SEQUENCE=GGGACGTCTCATTTTCGACTC
PRIMER_RIGHT_4_SEQUENCE=CGAAAGGACTTGACTGCATTTT
PRIMER_LEFT_4=4,21
PRIMER_RIGHT_4=113,22
PRIMER_LEFT_4_TM=61.014
PRIMER_RIGHT_4_TM=60.642
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_00726 Ps_contig_mira-and-tgicl-ass_franssen_7113
SEQUENCE=TCAAATTGGTTTCTATGAATTAGTCCACTGCACTCCCTTTTCCTAAAGCACAAGATCAATTCTTGGTACAGAAATTTCCAATGCTAAGTTGAGTCAAAACAAGCCTCAAATGGTGCCACTGATTCCACTTTCTTTAGTTTCCTGTCTGAAGCCTTCTTAAGGAAATCACGAATGAATGGCTTGTAAACAGAAGTTTGCAATTCAGTGAAAGGAGTTATAGTACTAATTTTGGTGCCACCATTTCCCTTTTTGTCAATGGACAACAAAGAAGGCTTTAAGTTCAAAACATGTCCATCAATCTTAATAGCTTTCACATTAATAAAATATTCATTAGAAGGAACACCTTCAACAGAAACAGCACCTGTTGAAACAGGGTTGACAATGAGTGGTGTGGTTTGAACAAATTTAGGCAATTTATCTGATCCAACAAGCAAGTTAGTGAATCCTTGTTTGTTTGAAGAAGGCAAACAAAGAGAGAATTTGTGCGGAAGGTTGTTTTTCAAAGCAAGCTGTTTGGGTAATGAAAGGTTTGATCTTGAAAGACCAATGATTCCTTTGGTGTTTTTTGGTAAGCCATTAAGTGCCGATTCATTGCCAGTGAATGATGGAAATGAATCAGTATCAATGCAACTTGAAAGCAAACCAGAAACCTTGTATTGAGAAATGAAAATGAAATCTTGACCAAGGCCACCACCAAATATGAATTTGGCTAATGAGTTGGTTGCAGAAGCAGGACATGTGTTGTTAGTGCAACCTGGTTTGAAGGGGCCATTGCAACCAATACATCCAATTTCTGGACATTGTTTAGAGCCACATTGAATAGGAGTATAGGATGATGAATTGTAGTGAGTATCACAGTCATACCAGAGATTTTCTCCTGCAAGATCAATGACTAGATTGAAATTTGTTCTAGGAGTTCCTATGCCAAGTGATGTATAGAAAAGATTGGTTGATGGATCTTTTCTGATGGGAAGATTGAAGGAGTTTGGTTGAGAGGATGCTGATAAAGAAGAAAGTGAGAAAAAGGATAGTGATATGAGGAAGAAATGGATGCTGAGAGAAGAAGAAGACATTGTGAATGATGAAGATATTGTTGTTGTGTCGCWATC
TARGET=1059,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00727 Ps_contig_mira-and-tgicl-ass_franssen_7122
SEQUENCE=CTACAATAATAGTCTAGAGTAACAGGTAATTATAGTTATCGGGTACTCATCTAAACTAAAACTAAAAAGAAGCATCTTTTGTGAAACTGCATCCACATATCCAATTGGGGAGGATTGGAGAGAGAGAKATCCAATGTTCCTAAATCAAATAATATAAGATTTTACTAGTAATCAACATATAGTGTCACCATTGTATGCAAGTCGAATGACAATCTTAATCTCCAATCCTTCTGTTTACGATTCAATGGCTTGCCTTTTTTTGTAGGATCTCTATTCTCTTTAACATCTGCTAATTTCCAAACACATGTCTCCGAGTCTTTTTCGATAGAATCTAAAAAGAATACTCCATCCACTTCACGGCAGTTGGACTCTATATTTTCTGTATAGGAAACTCTCATTGGCATGTCTTCAAATCGAGTGAGATCATCTGGCACTTTCAGTATCCTATCAGCACCCGGGGAAGAAACCTCGAGAGCCAAATCATCAGGTATGTCTCCAAGTGCTCCAACTTCATCTAATCTTTTCTTGAATTCCTGATTGTAACTCTCAAGCTCTTCCATGCTTGGGCATCCATATTCGCTTGTTAATTTGTCCAATCTCACGTAAACNTATCCACGAGG
TARGET=118,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00728 Ps_contig_mira-and-tgicl-ass_franssen_7127
SEQUENCE=TGACTATGAAGGTGAGGATGAAGTTCAGGAGGAGGAGGAGGAAGCTTAAACACTAGTATTTTGAACAGTGTGTTTTGTTTGTGTTGTGATATTAATGTGGTGTTTTCTCCCTCAACCTCAACCTCAACCTCAATTCCTTTTCTGGGTTTAACATTTTGGACACTTTGCTTGTGTTGCTATGGTAGTATTAGAGCGTACTTTTCTTGCATGTTTTAATTGCTGTTAAAAGGTGCTTATAAATTTCTTGGGTTTAAACTWAAAAATTT
TARGET=27,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00729 Ps_contig_mira-and-tgicl-ass_franssen_7155
SEQUENCE=AATGTTTGATATTAAGGAGTGAACTAAGTTTATTCTTATCTGCAGCTAAATTTAATACGTTTTTATTCCAAAAAATTCAAAACTCAATTAGCTTTTTTTATTTATTTATTTCACTTGTTCAAATTCCAACCTAGCTGTTTTCTTGTGATTTTCTCTCTGAGATTTCCTGTTCCACGTTTTGGAAATTGAACTTTCCTTTTTCATCACTTCATGGTTACCTAATTTGGTAAATTGAGATTAACTGCTTTTCAGACAAGTTTTCTAGTGGAATCATAGCTCAAAATTTGTGATTGCAATAAGGGAATGTTTTCATTATGTATTAGGGCAAAAATCTGTGATTGGAGTGTACTGTTTAGTTGAGAATGGATTATACTGTTTTGTTGTTGGTGATATTGTTGCAATTGTCAACAGTTTTTGGGTCTGGAGATGTTTTACAGACAAAGGATTGTGGCGATAATTCGGTAGCGAGTTCGATTTCTAGTCATGGTGAGGAATTGTTTTACTTAAATGGGAATGTTGTGAACAAAGTTGGTTTCTGTAAAGCTCTGAAATTGTATATTGCAAACGGTTGTGATGTGAATGACTACTTTGAAAGCAATCACTGTGTTTTGGATTTGGATTTGGATTTAGATGTCTCTTTTGTGAATTTGCCTTCAAAGGGGGGAAGGAAACTTTTGCAGAAAGGTTTGAACAATAATTCCACATCACAAGGAGATTCTAAGCATGTGTCAAGCAACCATGTTGGAATTTKTGTAGGTGGAGCTTTGTTGGTATGTTGTGCTGTTGTTTGTCCTTGTTTCTATGGGAAGAGAAGGAANACACCGCTCATGCTGTCTGGAAAGCTCAATCATGGATTGCTTCTCTTGA
TARGET=97,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00730 Ps_contig_mira-and-tgicl-ass_franssen_7161
SEQUENCE=GAGGTAGAGCTTTGTGACAATTCCTTAAAATTTCTACGCAATGTTCATCTGACCAATTATGGCATACGGCCTACAATGAAATCTAGAGTTAAATAGTATTTTGATAAGCAATATATATATTAAAGATTAGTTTTTATGTTTATATACCAACCTTAAGTATTATGGCATCTCCTTGTGGAATACTCTCAAACATGCTTCCTCCAACCATGTTCAATACCTATATAAAATTTGG
TARGET=111,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2026
PRIMER_LEFT_PENALTY=0.170825
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=TGTTCATCTGACCAATTATGGC
PRIMER_RIGHT_SEQUENCE=GAGTATTCCACAAGGAGATGCC
PRIMER_LEFT=41,22
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=59.829
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=0.2803
PRIMER_LEFT_1_PENALTY=0.170825
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=GTTCATCTGACCAATTATGGCA
PRIMER_RIGHT_1_SEQUENCE=AGGTATTGAACATGGTTGGAGG
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=218,22
PRIMER_LEFT_1_TM=59.829
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.2803
PRIMER_LEFT_2_PENALTY=0.170825
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=TGTTCATCTGACCAATTATGGC
PRIMER_RIGHT_2_SEQUENCE=AGGTATTGAACATGGTTGGAGG
PRIMER_LEFT_2=41,22
PRIMER_RIGHT_2=218,22
PRIMER_LEFT_2_TM=59.829
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=0.6014
PRIMER_LEFT_3_PENALTY=0.569606
PRIMER_RIGHT_3_PENALTY=0.031769
PRIMER_LEFT_3_SEQUENCE=AATTATGGCATACGGCCTACAA
PRIMER_RIGHT_3_SEQUENCE=GAGTATTCCACAAGGAGATGCC
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=60.570
PRIMER_RIGHT_3_TM=59.968
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=0.7321
PRIMER_LEFT_4_PENALTY=0.700295
PRIMER_RIGHT_4_PENALTY=0.031769
PRIMER_LEFT_4_SEQUENCE=GCATACGGCCTACAATGAAATC
PRIMER_RIGHT_4_SEQUENCE=GAGTATTCCACAAGGAGATGCC
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=184,22
PRIMER_LEFT_4_TM=60.700
PRIMER_RIGHT_4_TM=59.968
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_00731 Ps_contig_mira-and-tgicl-ass_franssen_7172
SEQUENCE=CTAAAACAATAATATGTGTTTTTCTAGCTACATAATATGTAACTGCNCCTATTTTTGTTTCTTAAATGACAATTATTTGCTTGTGTTAAGAGTCTGGCACCTTTAGGATTTTCTTTGGTACTTCCTGTTGTTGAGACACGTATATGGGTGAGTGAGTGAGTTTCTCTTAAGTGATGTTCTTTTTCCTTGTAGCCTATAAAATAAAACTTGTAACTAATTGTTTTGAGTTTAATTCTGATATAGTATTTGTAATAGTATTTTTTTCTATATTGATAATTTATCATAATTCAAT
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.8267
PRIMER_LEFT_PENALTY=0.437791
PRIMER_RIGHT_PENALTY=2.388959
PRIMER_LEFT_SEQUENCE=TTGCTTGTGTTAAGAGTCTGGC
PRIMER_RIGHT_SEQUENCE=GGCTACAAGGAAAAAGAACATCAC
PRIMER_LEFT=76,22
PRIMER_RIGHT=193,24
PRIMER_LEFT_TM=59.562
PRIMER_RIGHT_TM=60.389
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=118
PRIMER_PAIR_PENALTY_1=3.3328
PRIMER_LEFT_1_PENALTY=0.943830
PRIMER_RIGHT_1_PENALTY=2.388959
PRIMER_LEFT_1_SEQUENCE=GCTTGTGTTAAGAGTCTGGCAC
PRIMER_RIGHT_1_SEQUENCE=GGCTACAAGGAAAAAGAACATCAC
PRIMER_LEFT_1=78,22
PRIMER_RIGHT_1=193,24
PRIMER_LEFT_1_TM=59.056
PRIMER_RIGHT_1_TM=60.389
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=116
PRIMER_PAIR_PENALTY_2=3.3647
PRIMER_LEFT_2_PENALTY=0.437791
PRIMER_RIGHT_2_PENALTY=2.926891
PRIMER_LEFT_2_SEQUENCE=TTGCTTGTGTTAAGAGTCTGGC
PRIMER_RIGHT_2_SEQUENCE=GGCTACAAGGAAAAAGAACATC
PRIMER_LEFT_2=76,22
PRIMER_RIGHT_2=193,22
PRIMER_LEFT_2_TM=59.562
PRIMER_RIGHT_2_TM=57.073
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=3.3814
PRIMER_LEFT_3_PENALTY=0.992475
PRIMER_RIGHT_3_PENALTY=2.388959
PRIMER_LEFT_3_SEQUENCE=TGCTTGTGTTAAGAGTCTGGCA
PRIMER_RIGHT_3_SEQUENCE=GGCTACAAGGAAAAAGAACATCAC
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=193,24
PRIMER_LEFT_3_TM=60.992
PRIMER_RIGHT_3_TM=60.389
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=117
PRIMER_PAIR_PENALTY_4=3.4065
PRIMER_LEFT_4_PENALTY=1.017590
PRIMER_RIGHT_4_PENALTY=2.388959
PRIMER_LEFT_4_SEQUENCE=GAGTCTGGCACCTTTAGGATTTT
PRIMER_RIGHT_4_SEQUENCE=GGCTACAAGGAAAAAGAACATCAC
PRIMER_LEFT_4=89,23
PRIMER_RIGHT_4=193,24
PRIMER_LEFT_4_TM=60.018
PRIMER_RIGHT_4_TM=60.389
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00732 Ps_contig_mira-and-tgicl-ass_franssen_7176
SEQUENCE=GTAGAGAGTAATAACATAGATGCAGTANATTTTTCTAGTTTCTAATCCTAATTGAATTGCATTATTGATACACTCAATGAATAAACTACTTTTCTAAAACATAACAGTGAAGATCAAAAGAAAAAGGAAACCGTTGTAAGTGAACTTTGATTCATCAGAGACACNGAAAAAATGTTCRTACAGTTTGTTTCAGAACAGTCGATTTAGAAGCAGCCGCCCAACAAAACTGCCTCAAATCTCCTCATTGGTGACCGGAGTTCTTTTGGGCTGAAGAGAGGCTTTTCCTCCTAATGTTGTCGAAGCATTTGAACTTGAAAAGAACGATAAACCGCCATCGCCTGTCGAAGACAAAGCTTGACCCTCAGAGGCAGAACCAGAGGATTTTAGGTGCCTGTTGGGGAACTTGATGCGAGGGATTCTCTTCAAAGCATGAGCACCACCCATCGTTGAAACAAAACAAATAACCTTCGAGAAATCAACACAACACAACACTTCTCCGAAATGTCTTAAGGCAGCCCCGATGACATTAACCTCTGTTTAGTATTTGTGCACGCCA
TARGET=477,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00733 Ps_contig_mira-and-tgicl-ass_franssen_7194
SEQUENCE=TCTCCACCACTTCCTGTTTCCAAACTSAATCATTACATTACTCACTCCCTCCTCCTCCTTCCTCCTCCTCTAAACAATCAAAAAACCAAACCAAACCTTCNTCACAATAA
TARGET=48,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00734 Ps_contig_mira-and-tgicl-ass_franssen_7194
SEQUENCE=TCTCCACCACTTCCTGTTTCCAAACTSAATCATTACATTACTCACTCCCTCCTCCTCCTTCCTCCTCCTCTAAACAATCAAAAAACCAAACCAAACCTTCNTCACAATAA
TARGET=83,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00735 Ps_contig_mira-and-tgicl-ass_franssen_7198
SEQUENCE=GGTTTTTTAGTCAACATCAAAACAATTGTTGCAACTATTAAGAAAAAATAATCAATTTGRCACACTATTCTACAAATAAAATAATGAAGCAAACTATTTACAAAGGAGTGTAGCTCATGGTTACATGTGACCACCTTATACAGATTACACATTTCTATGAATATTTTCCTCCCTCCTTTCACTTTTTCATGTGAAAATTCAAGTAACATTATGACTAAAAATTAAAATGTTTTTCCTAGCTTGTTGCCACATCAACTTTTCTTATATGAACACAAGTATTTGACTGAATTCTTCATCATGAATATACAGTTTAGATGGAAGATGCCATAAATTTGCGAAATCTTTCCTCTCTTTCCCTGCGAGACCGAGAAGATGATGTACTTGATGATGATGATGATGACGAGGTTCGTCTTTTCCTTCTTGGGCGGAAGAAAAAATCTTGTGTGTCAAGAACATAAGAAATCTTGGATGAGTTGCAAGATATCTTGCATTCCAATCCATTAACTACTTTGACATCTTCCATTACAGCGCAAATTGCAGAAAGCTCATCTAAATCCCCGTATTTCGATTTGTTTCTTTCTAGTAAAATGCCATGAGGCTCCTTTTTGCTAGGTAGAACCGACAGAAAATCATTGTGCAGATAAAAAGAAAAATTTGGATCAACAGGAACAGCAGCAATCTCGGGTTTTTCATTCATATTGTCCATGAGTTGTATGGAGAGTCGAGAGGGGCTAGACGAACATTGAAATCGATATATGTTTTCGTCATGAAGGATGACATGTGCGTTTGAATGATATACTGAATCATTTAATTTGTCTGGTTTCCGAGACTTTTCGTACTCGTATAATTGGAGAAGCTTATTGTCTTCCTCATCGGTTGCAACAGTTTGAAGCTGTCTGATAAGTTTATAGATTAACTTGTCCAAGGTGAATAACACATAAGACTGGTTTCCAAGAATTGCTCGACATTCATCTTCAAACTTAGCATTCTCAACGGATCCATCGAGCAAATTATATAAYGCGTCCATGAATCTTGCATAAGGATCCGTAGAACTAGCATCTTTTGCTTTCCGTTTCATTTCAGCAGTAGTTGAATTTTCTTTTGCAGATAAGATCCTTTCGTAAAGAATTTGATGAAGCCTAAAAAGCGCAAAGAAATCATCATTTCCGTAAAACACCCTTGAATCCTTCACATCTTCAACGAATGAAACGGCTGATACATGCTTTGTGAGAGGTTTCACAGTCGAAAGGAACCTCTCTGATAACGGTAAAGATGAGCCATCTCCACCATTTTGTGCATCACACATCCCCTCAGCCTCACCTTCACTCTCAGCTTTACCATCAACATCATCATGTTCCATATCTTCCTCCTCCTCGTGATCTCCCCGGGAACATTCATCACCAGGAGATTCACTTCCCGACACATCTTCGCCAGCTTCAGAAACATTTTCGCTATCTTCATCATCAGCAT
TARGET=383,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00736 Ps_contig_mira-and-tgicl-ass_franssen_7205
SEQUENCE=GTGACAATCACCATATAATCTAGCAAACATTGTCCATACAAATCAAATAGCAGTACACCCTATTATTGCATTACCATAAGAAACTTAACATGCTACAAGCTAGTTCACCAAGTATTTTCTAACAATAACAACTTCAGCTTAAAAAAATAATAATAATTGGCATGACCAACAAGATGATCAACGGATCCAAGTTCGTAATTACAATCAAATAACAATCATCCAGTAATTATTTTGCAATTTT
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6898
PRIMER_LEFT_PENALTY=1.522538
PRIMER_RIGHT_PENALTY=0.167242
PRIMER_LEFT_SEQUENCE=CATGCTACAAGCTAGTTCACCAA
PRIMER_RIGHT_SEQUENCE=ACTTGGATCCGTTGATCATCTT
PRIMER_LEFT=88,23
PRIMER_RIGHT=191,22
PRIMER_LEFT_TM=59.477
PRIMER_RIGHT_TM=59.833
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=1.7592
PRIMER_LEFT_1_PENALTY=1.522538
PRIMER_RIGHT_1_PENALTY=0.236697
PRIMER_LEFT_1_SEQUENCE=CATGCTACAAGCTAGTTCACCAA
PRIMER_RIGHT_1_SEQUENCE=AATTACGAACTTGGATCCGTTG
PRIMER_LEFT_1=88,23
PRIMER_RIGHT_1=199,22
PRIMER_LEFT_1_TM=59.477
PRIMER_RIGHT_1_TM=60.237
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=112
PRIMER_PAIR_PENALTY_2=2.0327
PRIMER_LEFT_2_PENALTY=1.865442
PRIMER_RIGHT_2_PENALTY=0.167242
PRIMER_LEFT_2_SEQUENCE=CATGCTACAAGCTAGTTCACCA
PRIMER_RIGHT_2_SEQUENCE=ACTTGGATCCGTTGATCATCTT
PRIMER_LEFT_2=88,22
PRIMER_RIGHT_2=191,22
PRIMER_LEFT_2_TM=58.135
PRIMER_RIGHT_2_TM=59.833
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=2.1021
PRIMER_LEFT_3_PENALTY=1.865442
PRIMER_RIGHT_3_PENALTY=0.236697
PRIMER_LEFT_3_SEQUENCE=CATGCTACAAGCTAGTTCACCA
PRIMER_RIGHT_3_SEQUENCE=AATTACGAACTTGGATCCGTTG
PRIMER_LEFT_3=88,22
PRIMER_RIGHT_3=199,22
PRIMER_LEFT_3_TM=58.135
PRIMER_RIGHT_3_TM=60.237
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=112
PRIMER_PAIR_PENALTY_4=2.1793
PRIMER_LEFT_4_PENALTY=2.012100
PRIMER_RIGHT_4_PENALTY=0.167242
PRIMER_LEFT_4_SEQUENCE=ACATGCTACAAGCTAGTTCACCA
PRIMER_RIGHT_4_SEQUENCE=ACTTGGATCCGTTGATCATCTT
PRIMER_LEFT_4=87,23
PRIMER_RIGHT_4=191,22
PRIMER_LEFT_4_TM=58.988
PRIMER_RIGHT_4_TM=59.833
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00737 Ps_contig_mira-and-tgicl-ass_franssen_7220
SEQUENCE=CGGGTTAGTTCTTACWYACTTTATCACATATATCATATCATGTACTATCATCATCATCATCTCTTCCTCTTTGTTTTTTTCACCCATGTATTTCAGGGTTCTTTCCTTCCTATTA
TARGET=47,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00738 Ps_contig_mira-and-tgicl-ass_franssen_7225
SEQUENCE=TACCGTTCGGGAGTCTGTTCGTAGCTTAGGTAGTCATGTTCAATTGGAAGCCATGGAAGCCGGAGATTNAGNTTACCCAAATCCGGCGAAATGAAGGAAAATCGGAGAGAAACCCTAGGTTGTTGATGAGAGAGGAAGACGAAGAAGCATTAGTGTAATTGATTTAGGGAAAAAAGAAATGGAAAATGCAGAGTTTGTTGGATGGAATTGAGAATAGGGTCGTTAGCGTGATAGTGAGAGAATGAAGAAGAAGAAAGAAGACACAGCACAATACAGCACGACACAACACAACGGAACGGAGAAGTTGAGCCTGTGTTGTGCGATGTGTGAGGC
TARGET=244,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1960
PRIMER_LEFT_PENALTY=0.149184
PRIMER_RIGHT_PENALTY=0.046801
PRIMER_LEFT_SEQUENCE=TTGAGAATAGGGTCGTTAGCGT
PRIMER_RIGHT_SEQUENCE=CACAACACAGGCTCAACTTCTC
PRIMER_LEFT=207,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=60.149
PRIMER_RIGHT_TM=59.953
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=113
PRIMER_PAIR_PENALTY_1=0.3147
PRIMER_LEFT_1_PENALTY=0.267873
PRIMER_RIGHT_1_PENALTY=0.046801
PRIMER_LEFT_1_SEQUENCE=GAGAGAGGAAGACGAAGAAGCA
PRIMER_RIGHT_1_SEQUENCE=CACAACACAGGCTCAACTTCTC
PRIMER_LEFT_1=127,22
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=60.268
PRIMER_RIGHT_1_TM=59.953
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.3147
PRIMER_LEFT_2_PENALTY=0.267873
PRIMER_RIGHT_2_PENALTY=0.046801
PRIMER_LEFT_2_SEQUENCE=TGAGAGAGGAAGACGAAGAAGC
PRIMER_RIGHT_2_SEQUENCE=CACAACACAGGCTCAACTTCTC
PRIMER_LEFT_2=126,22
PRIMER_RIGHT_2=319,22
PRIMER_LEFT_2_TM=60.268
PRIMER_RIGHT_2_TM=59.953
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.3430
PRIMER_LEFT_3_PENALTY=0.296177
PRIMER_RIGHT_3_PENALTY=0.046801
PRIMER_LEFT_3_SEQUENCE=TGAAGGAAAATCGGAGAGAAAC
PRIMER_RIGHT_3_SEQUENCE=CACAACACAGGCTCAACTTCTC
PRIMER_LEFT_3=91,22
PRIMER_RIGHT_3=319,22
PRIMER_LEFT_3_TM=59.704
PRIMER_RIGHT_3_TM=59.953
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=0.4627
PRIMER_LEFT_4_PENALTY=0.415851
PRIMER_RIGHT_4_PENALTY=0.046801
PRIMER_LEFT_4_SEQUENCE=GAAGGAAAATCGGAGAGAAACC
PRIMER_RIGHT_4_SEQUENCE=CACAACACAGGCTCAACTTCTC
PRIMER_LEFT_4=92,22
PRIMER_RIGHT_4=319,22
PRIMER_LEFT_4_TM=60.416
PRIMER_RIGHT_4_TM=59.953
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_00739 Ps_contig_mira-and-tgicl-ass_franssen_7240
SEQUENCE=GGGGATACTATATAAACCATAAAAGAAAAAGAATAGTAAAATTAGAAGKTCCTCCACCACTAAACAAAACCAATCCATTCCATTCAACTTCACAATCCACCCAATCCAACCCAAAGAAAAAAGAAACCAGCACTCTCCATAAATCAATGGCTACAACCAAGCTTCTATTATCATTCCCTCTCTTCCAACTGCAACTCCCTCCCCACCACCACCACCACCGCCGCCGCCGCTGCCAAAAATCAACTTCCCTTCATTCTACGTTTCCAGCACCGTAATGGATTCTCCCCACTCCGCT
TARGET=203,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00740 Ps_contig_mira-and-tgicl-ass_franssen_7240
SEQUENCE=GGGGATACTATATAAACCATAAAAGAAAAAGAATAGTAAAATTAGAAGKTCCTCCACCACTAAACAAAACCAATCCATTCCATTCAACTTCACAATCCACCCAATCCAACCCAAAGAAAAAAGAAACCAGCACTCTCCATAAATCAATGGCTACAACCAAGCTTCTATTATCATTCCCTCTCTTCCAACTGCAACTCCCTCCCCACCACCACCACCACCGCCGCCGCCGCTGCCAAAAATCAACTTCCCTTCATTCTACGTTTCCAGCACCGTAATGGATTCTCCCCACTCCGCT
TARGET=218,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00741 Ps_contig_mira-and-tgicl-ass_franssen_7246
SEQUENCE=TCAACGCAGAGTACGCGGGGAAACAACAACAGTAGAAAAATAGGGTACTAGAAGAGCAACAAGRACAAGAGAAAAAAAAAACCTGTGAGCACAAGAATTAAATGATTCAGATCGTKWGATTCTAAACCGAATGAACTTTCAGTTAGTTTCTCCTNTGTCGTTAGCGTACTAATTTGTTTTTGTTTTGTTTTGTTTTGTTTCGTTTTGTTTCACGTTTACCCAAATTCCGTGTGTCTTTCACTNTTTCNCACCAATAATATACTCT
TARGET=178,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00742 Ps_contig_mira-and-tgicl-ass_franssen_7247
SEQUENCE=GAACAACGCAGAGTACACGGGGAGGGTACTAGAAGARGCAACAAGAACAAGAGAAAAAAAAACCTAGTGAGCACAAGAATTAAATGATTCAGATCGTGAGATTCTAAACCGAATGAACTTTCAGTAGTTTCTCCTCTGTCGTTTAGCGTCAATTGTTTTTGTTTTGTTTTGTTTTGTTTCGTTTTGTTTTCAGTTTCCCAAATCCGTGTGCGTTTTCACATTTTCTCACCCAATAAATATAACTCTCTGAAGAAGTTTGTTTTTCAACTGGTTTTTGTTATGGTGTTCAAGTTTTGAAAATTTTCAGGTTTTAAGGTTGTTACAGAAATGGAGTGTTGGAGCTCGGTGGATGGGGTGGTTGGAGAGATAATGAGGATTCATAGATCTTTGCCGGTTAGGCCTGGGATTGATGAAGTTGAAGCAGCGAAAGGTTTGGTTATGAATGTTGAGAAGATGAACCTAAATTTAAGTTGGAATCTATAGAAGAAACAAAGGAAAGGAAATGATGTGTCTGGAGGTAGTTCTTTT
TARGET=157,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00743 Ps_contig_mira-and-tgicl-ass_franssen_7248
SEQUENCE=AACAAAAACAATTGACGCTAAACGACAGAGGAGAAACTACTGAAAGTTCATTCGGTTTAGAATCTCACGATCTGAATCATTTAATTCTTGTGCTCACAGGTTTTTTTTTTCTCTTGTTCTTGTTGCTCTTCTAGTACCCTATTTTTCTACTGTTGTTGTTGTTCCTTCTTCTAGTCGTTGCTGGTTTTGTTTTATGCGTTACTTTTCATATTTCCGAATATACCCTTATCTATTTTGCAAAATAACCACGTACGCCATTCACTGGTTTTTGTTTTTCTCCCACGCCCGCGGTTTTTTAATTAACGGCTTCTATCAAACTTGATTTATGAATTATGACATAAGTGCCGTTTTTATTTTATTTCCAAAAATAAAAATAAAATAAAACTTTTGTATTTGGCCC
TARGET=151,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1157
PRIMER_LEFT_PENALTY=0.058853
PRIMER_RIGHT_PENALTY=0.056850
PRIMER_LEFT_SEQUENCE=CAATTGACGCTAAACGACAGAG
PRIMER_RIGHT_SEQUENCE=ACCAGCAACGACTAGAAGAAGG
PRIMER_LEFT=8,22
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=59.941
PRIMER_RIGHT_TM=59.943
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.1727
PRIMER_LEFT_1_PENALTY=0.058853
PRIMER_RIGHT_1_PENALTY=0.113803
PRIMER_LEFT_1_SEQUENCE=CAATTGACGCTAAACGACAGAG
PRIMER_RIGHT_1_SEQUENCE=GTGGGAGAAAAACAAAAACCAG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=282,22
PRIMER_LEFT_1_TM=59.941
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.1727
PRIMER_LEFT_2_PENALTY=0.058853
PRIMER_RIGHT_2_PENALTY=0.113803
PRIMER_LEFT_2_SEQUENCE=CAATTGACGCTAAACGACAGAG
PRIMER_RIGHT_2_SEQUENCE=GGGAGAAAAACAAAAACCAGTG
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=280,22
PRIMER_LEFT_2_TM=59.941
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.1935
PRIMER_LEFT_3_PENALTY=0.058853
PRIMER_RIGHT_3_PENALTY=0.134602
PRIMER_LEFT_3_SEQUENCE=CAATTGACGCTAAACGACAGAG
PRIMER_RIGHT_3_SEQUENCE=GAATGGCGTACGTGGTTATTTT
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=59.941
PRIMER_RIGHT_3_TM=60.135
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.2442
PRIMER_LEFT_4_PENALTY=0.187326
PRIMER_RIGHT_4_PENALTY=0.056850
PRIMER_LEFT_4_SEQUENCE=ACAATTGACGCTAAACGACAGA
PRIMER_RIGHT_4_SEQUENCE=ACCAGCAACGACTAGAAGAAGG
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=184,22
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_00744 Ps_contig_mira-and-tgicl-ass_franssen_7257
SEQUENCE=AAGCAGTGGTATCACGCAGAGTGAGTGGTCTTATAGGACCTGCAAGAACGCAGAGATTAGTGAATGGAGCATTAGTACTAATCACTCTGGGAGGAAATGTATTTTGTGAAATTAATTTATTTATTTTGGTGTCCTTTATTCTGACAAGATCGCG
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1543
PRIMER_LEFT_PENALTY=0.062823
PRIMER_RIGHT_PENALTY=1.091518
PRIMER_LEFT_SEQUENCE=TATCACGCAGAGTGAGTGGTCT
PRIMER_RIGHT_SEQUENCE=CGCGATCTTGTCAGAATAAAGG
PRIMER_LEFT=9,22
PRIMER_RIGHT=153,22
PRIMER_LEFT_TM=59.937
PRIMER_RIGHT_TM=61.092
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=1.2928
PRIMER_LEFT_1_PENALTY=0.201251
PRIMER_RIGHT_1_PENALTY=1.091518
PRIMER_LEFT_1_SEQUENCE=GTATCACGCAGAGTGAGTGGTC
PRIMER_RIGHT_1_SEQUENCE=CGCGATCTTGTCAGAATAAAGG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=153,22
PRIMER_LEFT_1_TM=59.799
PRIMER_RIGHT_1_TM=61.092
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=1.4317
PRIMER_LEFT_2_PENALTY=0.340219
PRIMER_RIGHT_2_PENALTY=1.091518
PRIMER_LEFT_2_SEQUENCE=GCAAGAACGCAGAGATTAGTGA
PRIMER_RIGHT_2_SEQUENCE=CGCGATCTTGTCAGAATAAAGG
PRIMER_LEFT_2=41,22
PRIMER_RIGHT_2=153,22
PRIMER_LEFT_2_TM=59.660
PRIMER_RIGHT_2_TM=61.092
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=1.5950
PRIMER_LEFT_3_PENALTY=0.062823
PRIMER_RIGHT_3_PENALTY=1.532165
PRIMER_LEFT_3_SEQUENCE=TATCACGCAGAGTGAGTGGTCT
PRIMER_RIGHT_3_SEQUENCE=GCGATCTTGTCAGAATAAAGGA
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=152,22
PRIMER_LEFT_3_TM=59.937
PRIMER_RIGHT_3_TM=58.468
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=1.6109
PRIMER_LEFT_4_PENALTY=0.519421
PRIMER_RIGHT_4_PENALTY=1.091518
PRIMER_LEFT_4_SEQUENCE=GCAGAGATTAGTGAATGGAGCA
PRIMER_RIGHT_4_SEQUENCE=CGCGATCTTGTCAGAATAAAGG
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=153,22
PRIMER_LEFT_4_TM=59.481
PRIMER_RIGHT_4_TM=61.092
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00745 Ps_contig_mira-and-tgicl-ass_franssen_7262
SEQUENCE=ATGGRAAAATAGCCAGAGGAAGAAGAAGAACAAGAAGATGAATTCTGGGGGGTTTGGAGGATTTGGAATATGAATATGATACTTCATTTTCAATGTTCTTTAATTGATTCAGTTGAAAGTATTTAGTTTTCAGTTGCATGTTGTTTTATGTATATACTACTACTACTACATAGAAAATTAAAATTTAAAAAAGTTAAGGGGGTTGAATTTAAATAAAAAAAGTT
TARGET=19,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00746 Ps_contig_mira-and-tgicl-ass_franssen_7262
SEQUENCE=ATGGRAAAATAGCCAGAGGAAGAAGAAGAACAAGAAGATGAATTCTGGGGGGTTTGGAGGATTTGGAATATGAATATGATACTTCATTTTCAATGTTCTTTAATTGATTCAGTTGAAAGTATTTAGTTTTCAGTTGCATGTTGTTTTATGTATATACTACTACTACTACATAGAAAATTAAAATTTAAAAAAGTTAAGGGGGTTGAATTTAAATAAAAAAAGTT
TARGET=155,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00747 Ps_contig_mira-and-tgicl-ass_franssen_7271
SEQUENCE=CAAGCTTCCAACTCTCATCATCTCTCTCTCTCTCTCTCTCTCTCAACTTATTTTCTAGTATCATCTTCATCACCCTWTBGATCAGTCATCAATCCATGGACACAACTCAATGGCCTCAGGAGATTATGGTGAAGCCATTAGCAACAAACACAAGTGAAAAGAAACCAAGACCAGAAAAACAACAAGCTGTTAATTGTCCAAGGTGCAACTCAATCAACACAAAATTCTGTTACTACAACAACTATAGCTTAACACAACCAAGATATTTCTGCAAAACATGTAGAAGGTACTGGACTCAAGGTGGATCCATTAGAAACATTCCCGTGGGAGGTGGAACAAGAAAAAACAACAAGGTTATTAGATCTTCTTCTTAACCTTGTTTCAAATCCAACAAAAAACCTAGTACCTAGTATTCTTGTTACTAGTTCTCAAAATCAAAAACATCATGAACAAGGACAAGATCTAAATTTGGATTTCACTTCTGTTTCTTCTCATAGTTTTTCAGCATTGGAGCTTCTTACTGGGATAACTGCTTCAACTACAAGGGGTTTTCATTCTTTTATGCCGGTTCAACTTCAAGGTGATTCCAACACTAGTAATATTGGGTTTCCTTTGCAGGATTTTAAGCAAGTGCCAATGAATTTTTGTTTAGATGGGATTGGTGGAAATGGTTATGGAAATGAAGGAAGGGTTTTGTTTCCTTTTGAGGATTTAAAACAGGATTTGGATCAGAATAACAATAAGGGAGATCAACAAGGTTATACAACTKGGGTTTTGGAATGGAATGTTGGGAGGAGGAGKAKRAGGATATAATGGTAATTAATTAAGGAAAAGTCATCTATATGTTTCATAGTATTGCTAACTTAATTTCTTCTTTCAATTTCTTGCACTAGTTGTTGTTGAATTATCTATAGGAATTAAGGATGAAAAAATAAATTGATCTTCAATTTTAGTGTTTAGGACAATTAGTGATGGAAGTAGAGATAATTTTATATTTTGTATGTTTTGTTTCTCATTGTTACTTCAACTTTTTTTTAGTSATGTTTGATGCATTCTC
TARGET=21,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00748 Ps_contig_mira-and-tgicl-ass_franssen_7272
SEQUENCE=GACTCACATTTCCTSTSAGAAAACATTCCCTTTTTCTTCACAAGCTTCCAACTCTCATCATCTCTCTCTCTCTCTCTCAACTTATTTTCAGTATCATCTTCATCACCCTTTGATCATCATCAATCCATGGACACAACTCAATGGCCTCAGGAGATTATGGTGAAGCCATTAGCAACAAACACAAGTAGAAAAGAAAMCMAAGACCAAGAAAAACAAACAAGCTAGTTAATTGTCCAAGG
TARGET=61,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00749 Ps_contig_mira-and-tgicl-ass_franssen_7302
SEQUENCE=CTTAGCCATCAAACTACTCAGTAAAAAACAAGATTCACACAATTCAACTAGTTAAACCGTATGAGTCAGTTATGCTTTTACAGTATTGCTAGAATCACCCCTCTTCATCATACAACAATTTGAGGCACGTATTTGATGTACTCACTTATTATTATTATTAATATTACAAGCACCGTTATTGTGTGCCTTGAGTTTCGCCGCCTTCACTTGATGGATCCCAACCAGGGGGTTTGATGTCCGGCCAATCACCACGACATCCCAATCTTATGTATAAGCCTTTTTTACCTGGAAGTTTCTTGTCAGATAGGATTAACTGTTTTAGGGCCCTCAAACATCCTTCTGCCACACTTTTAGCATAGGTAGGATCCCATCCCTGTAGCTTGACAACAACATCTGATTTTGTGTATGCAGTTATGCCATCTTTGACATTTCGGTGTGTCTCTTCCTTTGCAGAATCTTCATCTGATGCTTCAGTCTGTGACAACCAGACAGTAAATCCTGCATAAAGATGTCTCCATTTATCAGATCTTGAAGCAGTACCATGTCTACCTCCTAATGTTGCAACTACTGTCCTTACATGGCTACTTGTGCTTTCAAGTACAGCGCCTTCACCCGCGTACTCTGCGTTTGTTATTTTTACCACTGCTT
TARGET=146,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1355
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=TGCTAGAATCACCCCTCTTCAT
PRIMER_RIGHT_SEQUENCE=AAAAGTGTGGCAGAAGGATGTT
PRIMER_LEFT=86,22
PRIMER_RIGHT=351,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.1872
PRIMER_LEFT_1_PENALTY=0.147295
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=CACCCCTCTTCATCATACAACA
PRIMER_RIGHT_1_SEQUENCE=AAAGTGTGGCAGAAGGATGTTT
PRIMER_LEFT_1=95,22
PRIMER_RIGHT_1=350,22
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.2611
PRIMER_LEFT_2_PENALTY=0.095574
PRIMER_RIGHT_2_PENALTY=0.165541
PRIMER_LEFT_2_SEQUENCE=TGCTAGAATCACCCCTCTTCAT
PRIMER_RIGHT_2_SEQUENCE=CGAAACTCAAGGCACACAATAA
PRIMER_LEFT_2=86,22
PRIMER_RIGHT_2=196,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.166
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=0.3065
PRIMER_LEFT_3_PENALTY=0.095574
PRIMER_RIGHT_3_PENALTY=0.210886
PRIMER_LEFT_3_SEQUENCE=TGCTAGAATCACCCCTCTTCAT
PRIMER_RIGHT_3_SEQUENCE=GCTAAAAGTGTGGCAGAAGGAT
PRIMER_LEFT_3=86,22
PRIMER_RIGHT_3=354,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.789
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=0.3128
PRIMER_LEFT_4_PENALTY=0.147295
PRIMER_RIGHT_4_PENALTY=0.165541
PRIMER_LEFT_4_SEQUENCE=CACCCCTCTTCATCATACAACA
PRIMER_RIGHT_4_SEQUENCE=CGAAACTCAAGGCACACAATAA
PRIMER_LEFT_4=95,22
PRIMER_RIGHT_4=196,22
PRIMER_LEFT_4_TM=59.853
PRIMER_RIGHT_4_TM=60.166
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=102
=
PRIMER_SEQUENCE_ID=SSR_00750 Ps_contig_mira-and-tgicl-ass_franssen_7317
SEQUENCE=TTCTTCTTCTTTCTATCTTCTANAAAGGAAAAAGAAAGACAATAATTACAGAAAATAAATAAACCAAGAAAAACAAACCTACCAAAAGAAGGGAACGTCGGTGGTTGTCTCAGTTGGAGAAAATACAGAATATTACATTCTCAGTTACATCATCATCATCATCCACCTCCTCCTCCTTAACTTCARCTACAATGAAGATCCAAGGTTACTGCAAACGCCATCATACTCACCTAATTCATATACTTTCCAACCTCACACTATGGCCTTACCT
TARGET=148,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00751 Ps_contig_mira-and-tgicl-ass_franssen_7317
SEQUENCE=TTCTTCTTCTTTCTATCTTCTANAAAGGAAAAAGAAAGACAATAATTACAGAAAATAAATAAACCAAGAAAAACAAACCTACCAAAAGAAGGGAACGTCGGTGGTTGTCTCAGTTGGAGAAAATACAGAATATTACATTCTCAGTTACATCATCATCATCATCCACCTCCTCCTCCTTAACTTCARCTACAATGAAGATCCAAGGTTACTGCAAACGCCATCATACTCACCTAATTCATATACTTTCCAACCTCACACTATGGCCTTACCT
TARGET=166,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00752 Ps_contig_mira-and-tgicl-ass_franssen_7319
SEQUENCE=TGGCTCCATCGTCCAGCTTCTCGATTACATATGCACTCTTTTCCAGAATCGCAAGATTATTATGGTCCTCAACATGGAATCCATAATACCTGATTGTGAGATCATTGGAAGTCCAAAGCCATTTGTGGATTCAGAATTACCAGTTCAGAAAGCATTGCGTGATAACACTGTGGGGAGTTCGATCAACAACAATAATAATAACAATTATAATAATAATAGTAATATTTTGGCTAATCAAAATACAGGTAGTACTAATGTGCAGAATTTCAGGCCAACAATTCAACCTTCATACAAACCT
TARGET=207,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0435
PRIMER_LEFT_PENALTY=0.067436
PRIMER_RIGHT_PENALTY=0.976051
PRIMER_LEFT_SEQUENCE=CCTGATTGTGAGATCATTGGAA
PRIMER_RIGHT_SEQUENCE=GGCCTGAAATTCTGCACATTAG
PRIMER_LEFT=88,22
PRIMER_RIGHT=272,22
PRIMER_LEFT_TM=59.933
PRIMER_RIGHT_TM=60.976
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=1.1559
PRIMER_LEFT_1_PENALTY=0.067436
PRIMER_RIGHT_1_PENALTY=1.088501
PRIMER_LEFT_1_SEQUENCE=CCTGATTGTGAGATCATTGGAA
PRIMER_RIGHT_1_SEQUENCE=GGCCTGAAATTCTGCACATTA
PRIMER_LEFT_1=88,22
PRIMER_RIGHT_1=272,21
PRIMER_LEFT_1_TM=59.933
PRIMER_RIGHT_1_TM=60.089
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=1.2191
PRIMER_LEFT_2_PENALTY=0.243031
PRIMER_RIGHT_2_PENALTY=0.976051
PRIMER_LEFT_2_SEQUENCE=ATAACACTGTGGGGAGTTCGAT
PRIMER_RIGHT_2_SEQUENCE=GGCCTGAAATTCTGCACATTAG
PRIMER_LEFT_2=161,22
PRIMER_RIGHT_2=272,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=60.976
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.3000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=1.2321
PRIMER_LEFT_3_PENALTY=0.143555
PRIMER_RIGHT_3_PENALTY=1.088501
PRIMER_LEFT_3_SEQUENCE=CTCTTTTCCAGAATCGCAAGAT
PRIMER_RIGHT_3_SEQUENCE=GGCCTGAAATTCTGCACATTA
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=272,21
PRIMER_LEFT_3_TM=59.856
PRIMER_RIGHT_3_TM=60.089
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=1.2480
PRIMER_LEFT_4_PENALTY=0.271999
PRIMER_RIGHT_4_PENALTY=0.976051
PRIMER_LEFT_4_SEQUENCE=GTTCAGAAAGCATTGCGTGATA
PRIMER_RIGHT_4_SEQUENCE=GGCCTGAAATTCTGCACATTAG
PRIMER_LEFT_4=142,22
PRIMER_RIGHT_4=272,22
PRIMER_LEFT_4_TM=60.272
PRIMER_RIGHT_4_TM=60.976
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=131
=
PRIMER_SEQUENCE_ID=SSR_00753 Ps_contig_mira-and-tgicl-ass_franssen_7322
SEQUENCE=CACCGATGGCATATGACGACACGCGCGAGGACGAGTAAAATTAGATTCAAAAATCGCGCAAGAAAATCGGATGAATCCGTGCCTTTCGAATTCCAAACGTAATCAATGCGCGAAATAGAAGCAATTTGAGGAAGACAAAAGACCTACCGGACTCGTCGATTCATTGTGTTGCGTTCATGGATGTTGATTCTTCAATTGGCCGTTGATGATGAAGAGTGAGTGAACACCGATATCCAACCCCATGAGTTTTTCTTCTTCTTCTTCCAAATTCTTGAAATCTCCTTCTTCCGATTTCAACTTCCCTAATCGCTCGTATCTCCTTCTACTGTTATCTCTTTCTCTGTTTTTGTTATTCTTCTGTTGAGGAACGAATTGAGTTTGAAGATTGAAGGATCAATCTGAATCGAAAGTTTGCAGGATCAATCTGAATCGAAAGTTTGTTATGGATTTTTCAGTTGCTCGCGTGGTGTTGGCTCGGTGAAGATTGAAGAAGAAAGAGGAGGTCCAAAAAAGAGGGAAAAGATTTGTGTACGTTTGGCTTATTGCAGCTTGTGGTTGAGTTTCGTCGTGCGTTGCCGTTTGCTTTGGAGCTGCTTTGTGTTGCAACGTCGTTTTGGATGCCATTTCATGTCGTTTGCAGCTTGCTATTTGTATTGCGTTGATGTG
TARGET=250,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2881
PRIMER_LEFT_PENALTY=0.219844
PRIMER_RIGHT_PENALTY=0.068302
PRIMER_LEFT_SEQUENCE=GCGAAATAGAAGCAATTTGAGG
PRIMER_RIGHT_SEQUENCE=TAGGGAAGTTGAAATCGGAAGA
PRIMER_LEFT=109,22
PRIMER_RIGHT=304,22
PRIMER_LEFT_TM=60.220
PRIMER_RIGHT_TM=60.068
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.4898
PRIMER_LEFT_1_PENALTY=0.140832
PRIMER_RIGHT_1_PENALTY=0.348937
PRIMER_LEFT_1_SEQUENCE=GTGAGTGAACACCGATATCCAA
PRIMER_RIGHT_1_SEQUENCE=ATACGAGCGATTAGGGAAGTTG
PRIMER_LEFT_1=215,22
PRIMER_RIGHT_1=315,22
PRIMER_LEFT_1_TM=59.859
PRIMER_RIGHT_1_TM=59.651
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.4941
PRIMER_LEFT_2_PENALTY=0.140832
PRIMER_RIGHT_2_PENALTY=0.353222
PRIMER_LEFT_2_SEQUENCE=TGAGTGAACACCGATATCCAAC
PRIMER_RIGHT_2_SEQUENCE=TGGACCTCCTCTTTCTTCTTCA
PRIMER_LEFT_2=216,22
PRIMER_RIGHT_2=506,22
PRIMER_LEFT_2_TM=59.859
PRIMER_RIGHT_2_TM=60.353
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.4941
PRIMER_LEFT_3_PENALTY=0.140832
PRIMER_RIGHT_3_PENALTY=0.353222
PRIMER_LEFT_3_SEQUENCE=GTGAGTGAACACCGATATCCAA
PRIMER_RIGHT_3_SEQUENCE=TGGACCTCCTCTTTCTTCTTCA
PRIMER_LEFT_3=215,22
PRIMER_RIGHT_3=506,22
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=60.353
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=292
PRIMER_PAIR_PENALTY_4=0.5160
PRIMER_LEFT_4_PENALTY=0.219844
PRIMER_RIGHT_4_PENALTY=0.296177
PRIMER_LEFT_4_SEQUENCE=GCGAAATAGAAGCAATTTGAGG
PRIMER_RIGHT_4_SEQUENCE=GAAGTTGAAATCGGAAGAAGGA
PRIMER_LEFT_4=109,22
PRIMER_RIGHT_4=300,22
PRIMER_LEFT_4_TM=60.220
PRIMER_RIGHT_4_TM=59.704
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_00754 Ps_contig_mira-and-tgicl-ass_franssen_7333
SEQUENCE=CAAATCCCAACAACAACAATCCCAACAACTAACACAGAAGGCATCGTTTTGTCACGTGACATCTTCATATCTGAAACCGATGAAGAAAACCAACTCGGCGAAGAAAACACTGTAATCGGTCTAGAAGACAACGTAATAAACTCGTACCCGAGGTTTCAATTCAACAGAGACAACGCTACCGTATTAGGACAGAACACAACATGTTCTATTTGTTTGTGTGAGTATAAAGATTCCGAGATGCTGAGGATGATGCCTGAGTGTCGTCATTATTTTCATCTCTGCTGTCTTGATTCGTGGTTGAAGCTTAACGGTTCTTGTCCGGTTTGCCGAAACTCCCCGCTTCCAACKCCGCTAGCTACTCCGCTTCAGGAGGTTGTTCCGCTTTCTCAGTTACGGTGCTGATCGGAGACGAAGAAGGTGTACTTTTTTTTCTTCATAATTATAGTTAGATTGAAAATTATTTAGTGNGGATTGAACTGAGGTGATGTATGAATTATTAGTAGTTTGTAGTGGAGGGAGGAGGTA
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00755 Ps_contig_mira-and-tgicl-ass_franssen_7334
SEQUENCE=GATGTCACGTGACAAAACGATGCCTTCTTGTTGTAGTTGTTGGGATTGTTGTTGTTGGGATTGTTGTTGTTGAGGTTGTTGTTGTTGTTGTGATTGGTGCGACAACAGAGGTAGAAAGAGAGGAAGAGAGTGGCGAGGAGGACGAGAAAAGCGAGGGTTATGGCTATGGAGTAGCCGAGGCCAAGGTTGGTGAAGTAACTGTGTGGAGAGGACATGGAGAAGGGAGAGGAAGAGGAAGAGGAGGAGGAGAAAAGGGATTCTCCATTTGTGGAGGGAGGGAAGAGAAATTGAGGGAGGAAAAGTTAAGGGGTTAGGAG
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2214
PRIMER_LEFT_PENALTY=0.193337
PRIMER_RIGHT_PENALTY=0.028056
PRIMER_LEFT_SEQUENCE=CGATGCCTTCTTGTTGTAGTTG
PRIMER_RIGHT_SEQUENCE=CCATGTCCTCTCCACACAGTTA
PRIMER_LEFT=17,22
PRIMER_RIGHT=216,22
PRIMER_LEFT_TM=59.807
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=200
PRIMER_PAIR_PENALTY_1=0.2364
PRIMER_LEFT_1_PENALTY=0.193337
PRIMER_RIGHT_1_PENALTY=0.043085
PRIMER_LEFT_1_SEQUENCE=CGATGCCTTCTTGTTGTAGTTG
PRIMER_RIGHT_1_SEQUENCE=TTCTCCTCCTCCTCTTCCTCTT
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=250,22
PRIMER_LEFT_1_TM=59.807
PRIMER_RIGHT_1_TM=59.957
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.2364
PRIMER_LEFT_2_PENALTY=0.193337
PRIMER_RIGHT_2_PENALTY=0.043085
PRIMER_LEFT_2_SEQUENCE=CGATGCCTTCTTGTTGTAGTTG
PRIMER_RIGHT_2_SEQUENCE=CTTCCTCTTCCTCTCCCTTCTC
PRIMER_LEFT_2=17,22
PRIMER_RIGHT_2=237,22
PRIMER_LEFT_2_TM=59.807
PRIMER_RIGHT_2_TM=59.957
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=221
PRIMER_PAIR_PENALTY_3=0.2364
PRIMER_LEFT_3_PENALTY=0.193337
PRIMER_RIGHT_3_PENALTY=0.043085
PRIMER_LEFT_3_SEQUENCE=CGATGCCTTCTTGTTGTAGTTG
PRIMER_RIGHT_3_SEQUENCE=TCTTCCTCTTCCTCTCCCTTCT
PRIMER_LEFT_3=17,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=59.807
PRIMER_RIGHT_3_TM=59.957
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.2364
PRIMER_LEFT_4_PENALTY=0.193337
PRIMER_RIGHT_4_PENALTY=0.043085
PRIMER_LEFT_4_SEQUENCE=CGATGCCTTCTTGTTGTAGTTG
PRIMER_RIGHT_4_SEQUENCE=CTCTTCCTCTTCCTCTCCCTTC
PRIMER_LEFT_4=17,22
PRIMER_RIGHT_4=239,22
PRIMER_LEFT_4_TM=59.807
PRIMER_RIGHT_4_TM=59.957
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_00756 Ps_contig_mira-and-tgicl-ass_franssen_7334
SEQUENCE=GATGTCACGTGACAAAACGATGCCTTCTTGTTGTAGTTGTTGGGATTGTTGTTGTTGGGATTGTTGTTGTTGAGGTTGTTGTTGTTGTTGTGATTGGTGCGACAACAGAGGTAGAAAGAGAGGAAGAGAGTGGCGAGGAGGACGAGAAAAGCGAGGGTTATGGCTATGGAGTAGCCGAGGCCAAGGTTGGTGAAGTAACTGTGTGGAGAGGACATGGAGAAGGGAGAGGAAGAGGAAGAGGAGGAGGAGAAAAGGGATTCTCCATTTGTGGAGGGAGGGAAGAGAAATTGAGGGAGGAAAAGTTAAGGGGTTAGGAG
TARGET=61,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1962
PRIMER_LEFT_PENALTY=0.168114
PRIMER_RIGHT_PENALTY=0.028056
PRIMER_LEFT_SEQUENCE=GTTGTTGGGATTGTTGTTGTTG
PRIMER_RIGHT_SEQUENCE=CCATGTCCTCTCCACACAGTTA
PRIMER_LEFT=35,22
PRIMER_RIGHT=216,22
PRIMER_LEFT_TM=60.168
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=0.2112
PRIMER_LEFT_1_PENALTY=0.168114
PRIMER_RIGHT_1_PENALTY=0.043085
PRIMER_LEFT_1_SEQUENCE=GTTGTTGGGATTGTTGTTGTTG
PRIMER_RIGHT_1_SEQUENCE=TTCTCCTCCTCCTCTTCCTCTT
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=250,22
PRIMER_LEFT_1_TM=60.168
PRIMER_RIGHT_1_TM=59.957
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=216
PRIMER_PAIR_PENALTY_2=0.2112
PRIMER_LEFT_2_PENALTY=0.168114
PRIMER_RIGHT_2_PENALTY=0.043085
PRIMER_LEFT_2_SEQUENCE=GTTGTTGGGATTGTTGTTGTTG
PRIMER_RIGHT_2_SEQUENCE=TTTCTCCTCCTCCTCTTCCTCT
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=251,22
PRIMER_LEFT_2_TM=60.168
PRIMER_RIGHT_2_TM=59.957
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=217
PRIMER_PAIR_PENALTY_3=0.2112
PRIMER_LEFT_3_PENALTY=0.168114
PRIMER_RIGHT_3_PENALTY=0.043085
PRIMER_LEFT_3_SEQUENCE=GTTGTTGGGATTGTTGTTGTTG
PRIMER_RIGHT_3_SEQUENCE=TCTTCCTCTTCCTCTCCCTTCT
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=60.168
PRIMER_RIGHT_3_TM=59.957
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.2112
PRIMER_LEFT_4_PENALTY=0.168114
PRIMER_RIGHT_4_PENALTY=0.043085
PRIMER_LEFT_4_SEQUENCE=GTTGTTGGGATTGTTGTTGTTG
PRIMER_RIGHT_4_SEQUENCE=CTTCCTCTTCCTCTCCCTTCTC
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=237,22
PRIMER_LEFT_4_TM=60.168
PRIMER_RIGHT_4_TM=59.957
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_00757 Ps_contig_mira-and-tgicl-ass_franssen_7334
SEQUENCE=GATGTCACGTGACAAAACGATGCCTTCTTGTTGTAGTTGTTGGGATTGTTGTTGTTGGGATTGTTGTTGTTGAGGTTGTTGTTGTTGTTGTGATTGGTGCGACAACAGAGGTAGAAAGAGAGGAAGAGAGTGGCGAGGAGGACGAGAAAAGCGAGGGTTATGGCTATGGAGTAGCCGAGGCCAAGGTTGGTGAAGTAACTGTGTGGAGAGGACATGGAGAAGGGAGAGGAAGAGGAAGAGGAGGAGGAGAAAAGGGATTCTCCATTTGTGGAGGGAGGGAAGAGAAATTGAGGGAGGAAAAGTTAAGGGGTTAGGAG
TARGET=75,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1962
PRIMER_LEFT_PENALTY=0.168114
PRIMER_RIGHT_PENALTY=0.028056
PRIMER_LEFT_SEQUENCE=GTTGTTGGGATTGTTGTTGTTG
PRIMER_RIGHT_SEQUENCE=CCATGTCCTCTCCACACAGTTA
PRIMER_LEFT=50,22
PRIMER_RIGHT=216,22
PRIMER_LEFT_TM=60.168
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.1962
PRIMER_LEFT_1_PENALTY=0.168114
PRIMER_RIGHT_1_PENALTY=0.028056
PRIMER_LEFT_1_SEQUENCE=GTTGTTGTTGGGATTGTTGTTG
PRIMER_RIGHT_1_SEQUENCE=CCATGTCCTCTCCACACAGTTA
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=216,22
PRIMER_LEFT_1_TM=60.168
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.1962
PRIMER_LEFT_2_PENALTY=0.168114
PRIMER_RIGHT_2_PENALTY=0.028056
PRIMER_LEFT_2_SEQUENCE=GTTGTTGGGATTGTTGTTGTTG
PRIMER_RIGHT_2_SEQUENCE=CCATGTCCTCTCCACACAGTTA
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=216,22
PRIMER_LEFT_2_TM=60.168
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=182
PRIMER_PAIR_PENALTY_3=0.2112
PRIMER_LEFT_3_PENALTY=0.168114
PRIMER_RIGHT_3_PENALTY=0.043085
PRIMER_LEFT_3_SEQUENCE=GTTGTTGGGATTGTTGTTGTTG
PRIMER_RIGHT_3_SEQUENCE=TTCTCCTCCTCCTCTTCCTCTT
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=250,22
PRIMER_LEFT_3_TM=60.168
PRIMER_RIGHT_3_TM=59.957
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=0.2112
PRIMER_LEFT_4_PENALTY=0.168114
PRIMER_RIGHT_4_PENALTY=0.043085
PRIMER_LEFT_4_SEQUENCE=GTTGTTGGGATTGTTGTTGTTG
PRIMER_RIGHT_4_SEQUENCE=TTTCTCCTCCTCCTCTTCCTCT
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=251,22
PRIMER_LEFT_4_TM=60.168
PRIMER_RIGHT_4_TM=59.957
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_00758 Ps_contig_mira-and-tgicl-ass_franssen_7334
SEQUENCE=GATGTCACGTGACAAAACGATGCCTTCTTGTTGTAGTTGTTGGGATTGTTGTTGTTGGGATTGTTGTTGTTGAGGTTGTTGTTGTTGTTGTGATTGGTGCGACAACAGAGGTAGAAAGAGAGGAAGAGAGTGGCGAGGAGGACGAGAAAAGCGAGGGTTATGGCTATGGAGTAGCCGAGGCCAAGGTTGGTGAAGTAACTGTGTGGAGAGGACATGGAGAAGGGAGAGGAAGAGGAAGAGGAGGAGGAGAAAAGGGATTCTCCATTTGTGGAGGGAGGGAAGAGAAATTGAGGGAGGAAAAGTTAAGGGGTTAGGAG
TARGET=238,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4410
PRIMER_LEFT_PENALTY=0.168114
PRIMER_RIGHT_PENALTY=0.272848
PRIMER_LEFT_SEQUENCE=GTTGTTGGGATTGTTGTTGTTG
PRIMER_RIGHT_SEQUENCE=TAACCCCTTAACTTTTCCTCCC
PRIMER_LEFT=50,22
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=60.168
PRIMER_RIGHT_TM=59.727
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.4410
PRIMER_LEFT_1_PENALTY=0.168114
PRIMER_RIGHT_1_PENALTY=0.272848
PRIMER_LEFT_1_SEQUENCE=GTTGTTGTTGGGATTGTTGTTG
PRIMER_RIGHT_1_SEQUENCE=TAACCCCTTAACTTTTCCTCCC
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=60.168
PRIMER_RIGHT_1_TM=59.727
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.4410
PRIMER_LEFT_2_PENALTY=0.168114
PRIMER_RIGHT_2_PENALTY=0.272848
PRIMER_LEFT_2_SEQUENCE=GTTGTTGGGATTGTTGTTGTTG
PRIMER_RIGHT_2_SEQUENCE=TAACCCCTTAACTTTTCCTCCC
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=312,22
PRIMER_LEFT_2_TM=60.168
PRIMER_RIGHT_2_TM=59.727
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.4662
PRIMER_LEFT_3_PENALTY=0.193337
PRIMER_RIGHT_3_PENALTY=0.272848
PRIMER_LEFT_3_SEQUENCE=CGATGCCTTCTTGTTGTAGTTG
PRIMER_RIGHT_3_SEQUENCE=TAACCCCTTAACTTTTCCTCCC
PRIMER_LEFT_3=17,22
PRIMER_RIGHT_3=312,22
PRIMER_LEFT_3_TM=59.807
PRIMER_RIGHT_3_TM=59.727
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.5703
PRIMER_LEFT_4_PENALTY=0.028056
PRIMER_RIGHT_4_PENALTY=0.542268
PRIMER_LEFT_4_SEQUENCE=TAACTGTGTGGAGAGGACATGG
PRIMER_RIGHT_4_SEQUENCE=AACCCCTTAACTTTTCCTCCCT
PRIMER_LEFT_4=195,22
PRIMER_RIGHT_4=311,22
PRIMER_LEFT_4_TM=60.028
PRIMER_RIGHT_4_TM=60.542
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=117
=
PRIMER_SEQUENCE_ID=SSR_00759 Ps_contig_mira-and-tgicl-ass_franssen_7335
SEQUENCE=GGAGTCTCTCCACAAAAACGCAGTATTAAATGCAGCAGATGAAACCTCACCCACCCTKACCCTTCAAAAAAAATTAAATCCCATCCTAACCCCTTAATTTCCTCCCTCAATTTCTCTTTCTCTCCCTCACAAATGGAGAATTCCCCTTTTTCTCCTCCTCCTCTTCCTCTTCCTCTCCCTTCTCCATGTCCTCTCCACACAGTTACTTCACCAACCTTGGCCTCGGCTACTCCATAGCCATAACCCTCGCTTTTCTCGT
TARGET=152,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00760 Ps_contig_mira-and-tgicl-ass_franssen_7343
SEQUENCE=ATTCAGTGAAGAATGAATGCTTTTCTTTTTCTTTCTTCAAAAAATGAAAATATCATAGATTTCTCATCATTTACAGTGTGTTATATGAAGTCAACCAAAAACGATAAAAATAATATATTTTTGCAGAAAATGGTCGTATCTGCATTCGTGGCATCCGAATATGGAATTTATTGTTTTTCTTAGTATAGCTGCTCAAGAAATTGTTTCCTAATGTGTCCAGTTTTTTCTTTCTTTCAAGTTTGTGACC
TARGET=224,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00761 Ps_contig_mira-and-tgicl-ass_franssen_7362
SEQUENCE=CGGAGGGCGGTGAGTCTCGCGATTTCGCGGCGGAAATTGAATCCAGGCGATGGCGGAGATAGCTCTAACGGTTCTGTAGAAATACGTGCGACGCGGCAACATGATGATGATGATGATGATGGAGAAGATGATAGAAGAGATAGTGATTTTGAAAAAGAGAGAGGTTGTTGTTGTTGTGATTGTAACTGAGAGTGTGAGTGTGAATTATCTAAGAGTGAAGGTTTGGGGCGGAAAGGGAAAAGGAACCGGTGGGTAGGGGGAAGAAGAGAAGTAGAGTGAAGCATGGTTTAATTGGAGAGGATTAATAAATGCAATAATGAATGAGAATATGGAGGAATATAAGAAGGGACGAAGATGAATTTGGATATTGAGAGAGAGAGAGAAGTATCAAGAGAAGATGCTAGGAGGATATACCATGAAGGACGAAGAGGGAGAGCTTGTTTGTTAGCCGTGAATCATTTCCATTTCCATTTCCATGTACTCTGCGTTGATAACCA
TARGET=101,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8566
PRIMER_LEFT_PENALTY=0.706271
PRIMER_RIGHT_PENALTY=0.150322
PRIMER_LEFT_SEQUENCE=GGTTCTGTAGAAATACGTGCGA
PRIMER_RIGHT_SEQUENCE=TATCCAAATTCATCTTCGTCCC
PRIMER_LEFT=69,22
PRIMER_RIGHT=366,22
PRIMER_LEFT_TM=59.294
PRIMER_RIGHT_TM=60.150
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=1.1131
PRIMER_LEFT_1_PENALTY=0.706271
PRIMER_RIGHT_1_PENALTY=0.406861
PRIMER_LEFT_1_SEQUENCE=GGTTCTGTAGAAATACGTGCGA
PRIMER_RIGHT_1_SEQUENCE=CAAATTCATCTTCGTCCCTTCT
PRIMER_LEFT_1=69,22
PRIMER_RIGHT_1=362,22
PRIMER_LEFT_1_TM=59.294
PRIMER_RIGHT_1_TM=59.593
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=1.2256
PRIMER_LEFT_2_PENALTY=1.075241
PRIMER_RIGHT_2_PENALTY=0.150322
PRIMER_LEFT_2_SEQUENCE=GGTTCTGTAGAAATACGTGCGAC
PRIMER_RIGHT_2_SEQUENCE=TATCCAAATTCATCTTCGTCCC
PRIMER_LEFT_2=69,23
PRIMER_RIGHT_2=366,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=60.150
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=1.2475
PRIMER_LEFT_3_PENALTY=0.706271
PRIMER_RIGHT_3_PENALTY=0.541246
PRIMER_LEFT_3_SEQUENCE=GGTTCTGTAGAAATACGTGCGA
PRIMER_RIGHT_3_SEQUENCE=CGTCCCTTCTTATATTCCTCCA
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=350,22
PRIMER_LEFT_3_TM=59.294
PRIMER_RIGHT_3_TM=59.459
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=1.3696
PRIMER_LEFT_4_PENALTY=0.706271
PRIMER_RIGHT_4_PENALTY=0.663326
PRIMER_LEFT_4_SEQUENCE=GGTTCTGTAGAAATACGTGCGA
PRIMER_RIGHT_4_SEQUENCE=ATCCTCTCCAATTAAACCATGC
PRIMER_LEFT_4=69,22
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=59.294
PRIMER_RIGHT_4_TM=59.337
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_00762 Ps_contig_mira-and-tgicl-ass_franssen_7362
SEQUENCE=CGGAGGGCGGTGAGTCTCGCGATTTCGCGGCGGAAATTGAATCCAGGCGATGGCGGAGATAGCTCTAACGGTTCTGTAGAAATACGTGCGACGCGGCAACATGATGATGATGATGATGATGGAGAAGATGATAGAAGAGATAGTGATTTTGAAAAAGAGAGAGGTTGTTGTTGTTGTGATTGTAACTGAGAGTGTGAGTGTGAATTATCTAAGAGTGAAGGTTTGGGGCGGAAAGGGAAAAGGAACCGGTGGGTAGGGGGAAGAAGAGAAGTAGAGTGAAGCATGGTTTAATTGGAGAGGATTAATAAATGCAATAATGAATGAGAATATGGAGGAATATAAGAAGGGACGAAGATGAATTTGGATATTGAGAGAGAGAGAGAAGTATCAAGAGAAGATGCTAGGAGGATATACCATGAAGGACGAAGAGGGAGAGCTTGTTTGTTAGCCGTGAATCATTTCCATTTCCATTTCCATGTACTCTGCGTTGATAACCA
TARGET=164,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8566
PRIMER_LEFT_PENALTY=0.706271
PRIMER_RIGHT_PENALTY=0.150322
PRIMER_LEFT_SEQUENCE=GGTTCTGTAGAAATACGTGCGA
PRIMER_RIGHT_SEQUENCE=TATCCAAATTCATCTTCGTCCC
PRIMER_LEFT=69,22
PRIMER_RIGHT=366,22
PRIMER_LEFT_TM=59.294
PRIMER_RIGHT_TM=60.150
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=1.1131
PRIMER_LEFT_1_PENALTY=0.706271
PRIMER_RIGHT_1_PENALTY=0.406861
PRIMER_LEFT_1_SEQUENCE=GGTTCTGTAGAAATACGTGCGA
PRIMER_RIGHT_1_SEQUENCE=CAAATTCATCTTCGTCCCTTCT
PRIMER_LEFT_1=69,22
PRIMER_RIGHT_1=362,22
PRIMER_LEFT_1_TM=59.294
PRIMER_RIGHT_1_TM=59.593
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=1.2256
PRIMER_LEFT_2_PENALTY=1.075241
PRIMER_RIGHT_2_PENALTY=0.150322
PRIMER_LEFT_2_SEQUENCE=GGTTCTGTAGAAATACGTGCGAC
PRIMER_RIGHT_2_SEQUENCE=TATCCAAATTCATCTTCGTCCC
PRIMER_LEFT_2=69,23
PRIMER_RIGHT_2=366,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=60.150
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=1.2475
PRIMER_LEFT_3_PENALTY=0.706271
PRIMER_RIGHT_3_PENALTY=0.541246
PRIMER_LEFT_3_SEQUENCE=GGTTCTGTAGAAATACGTGCGA
PRIMER_RIGHT_3_SEQUENCE=CGTCCCTTCTTATATTCCTCCA
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=350,22
PRIMER_LEFT_3_TM=59.294
PRIMER_RIGHT_3_TM=59.459
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=1.3696
PRIMER_LEFT_4_PENALTY=0.706271
PRIMER_RIGHT_4_PENALTY=0.663326
PRIMER_LEFT_4_SEQUENCE=GGTTCTGTAGAAATACGTGCGA
PRIMER_RIGHT_4_SEQUENCE=ATCCTCTCCAATTAAACCATGC
PRIMER_LEFT_4=69,22
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=59.294
PRIMER_RIGHT_4_TM=59.337
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_00763 Ps_contig_mira-and-tgicl-ass_franssen_7362
SEQUENCE=CGGAGGGCGGTGAGTCTCGCGATTTCGCGGCGGAAATTGAATCCAGGCGATGGCGGAGATAGCTCTAACGGTTCTGTAGAAATACGTGCGACGCGGCAACATGATGATGATGATGATGATGGAGAAGATGATAGAAGAGATAGTGATTTTGAAAAAGAGAGAGGTTGTTGTTGTTGTGATTGTAACTGAGAGTGTGAGTGTGAATTATCTAAGAGTGAAGGTTTGGGGCGGAAAGGGAAAAGGAACCGGTGGGTAGGGGGAAGAAGAGAAGTAGAGTGAAGCATGGTTTAATTGGAGAGGATTAATAAATGCAATAATGAATGAGAATATGGAGGAATATAAGAAGGGACGAAGATGAATTTGGATATTGAGAGAGAGAGAGAAGTATCAAGAGAAGATGCTAGGAGGATATACCATGAAGGACGAAGAGGGAGAGCTTGTTTGTTAGCCGTGAATCATTTCCATTTCCATTTCCATGTACTCTGCGTTGATAACCA
TARGET=370,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1996
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.165541
PRIMER_LEFT_SEQUENCE=AGCATGGTTTAATTGGAGAGGA
PRIMER_RIGHT_SEQUENCE=TGATTCACGGCTAACAAACAAG
PRIMER_LEFT=279,22
PRIMER_RIGHT=457,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.166
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.2453
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.211181
PRIMER_LEFT_1_SEQUENCE=AGCATGGTTTAATTGGAGAGGA
PRIMER_RIGHT_1_SEQUENCE=ATGGAAATGATTCACGGCTAAC
PRIMER_LEFT_1=279,22
PRIMER_RIGHT_1=464,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.211
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=0.3159
PRIMER_LEFT_2_PENALTY=0.150322
PRIMER_RIGHT_2_PENALTY=0.165541
PRIMER_LEFT_2_SEQUENCE=GGGACGAAGATGAATTTGGATA
PRIMER_RIGHT_2_SEQUENCE=TGATTCACGGCTAACAAACAAG
PRIMER_LEFT_2=345,22
PRIMER_RIGHT_2=457,22
PRIMER_LEFT_2_TM=60.150
PRIMER_RIGHT_2_TM=60.166
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=0.3615
PRIMER_LEFT_3_PENALTY=0.150322
PRIMER_RIGHT_3_PENALTY=0.211181
PRIMER_LEFT_3_SEQUENCE=GGGACGAAGATGAATTTGGATA
PRIMER_RIGHT_3_SEQUENCE=ATGGAAATGATTCACGGCTAAC
PRIMER_LEFT_3=345,22
PRIMER_RIGHT_3=464,22
PRIMER_LEFT_3_TM=60.150
PRIMER_RIGHT_3_TM=60.211
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=0.3969
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.362762
PRIMER_LEFT_4_SEQUENCE=AGCATGGTTTAATTGGAGAGGA
PRIMER_RIGHT_4_SEQUENCE=ATCAACGCAGAGTACATGGAAA
PRIMER_LEFT_4=279,22
PRIMER_RIGHT_4=491,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.637
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_00764 Ps_contig_mira-and-tgicl-ass_franssen_7365
SEQUENCE=GAAGATGAGAATCTGTGCAAATAATAATAATGAATGATGAAAATGTTGAAGGACACAGTGATGGTAACATGCAATAGAAGCAGAAAAGAAGAAGGACTAGTCTTGAGTAGTTTCATGAGATAGCTATATTGCTGACACTGTGTACTACTACTACTACTAACTCAAAGTTGCTTTGTTTGTTTTGAGTCTTGAAATTTTGCTTTGTTTTAGAAGTTTTGCGTTTTGTCCCTGTTGATCAAGTTGCCTGATACGTGAAAGACATTGAATGCAGTGTTAAA
TARGET=20,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00765 Ps_contig_mira-and-tgicl-ass_franssen_7365
SEQUENCE=GAAGATGAGAATCTGTGCAAATAATAATAATGAATGATGAAAATGTTGAAGGACACAGTGATGGTAACATGCAATAGAAGCAGAAAAGAAGAAGGACTAGTCTTGAGTAGTTTCATGAGATAGCTATATTGCTGACACTGTGTACTACTACTACTACTAACTCAAAGTTGCTTTGTTTGTTTTGAGTCTTGAAATTTTGCTTTGTTTTAGAAGTTTTGCGTTTTGTCCCTGTTGATCAAGTTGCCTGATACGTGAAAGACATTGAATGCAGTGTTAAA
TARGET=143,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1175
PRIMER_LEFT_PENALTY=0.109200
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=ATGTTGAAGGACACAGTGATGG
PRIMER_RIGHT_SEQUENCE=CAACTTGATCAACAGGGACAAA
PRIMER_LEFT=42,22
PRIMER_RIGHT=242,22
PRIMER_LEFT_TM=59.891
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=201
PRIMER_PAIR_PENALTY_1=0.2849
PRIMER_LEFT_1_PENALTY=0.109200
PRIMER_RIGHT_1_PENALTY=0.175676
PRIMER_LEFT_1_SEQUENCE=ATGTTGAAGGACACAGTGATGG
PRIMER_RIGHT_1_SEQUENCE=ACTGCATTCAATGTCTTTCACG
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=271,22
PRIMER_LEFT_1_TM=59.891
PRIMER_RIGHT_1_TM=60.176
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.4720
PRIMER_LEFT_2_PENALTY=0.109200
PRIMER_RIGHT_2_PENALTY=0.362762
PRIMER_LEFT_2_SEQUENCE=ATGTTGAAGGACACAGTGATGG
PRIMER_RIGHT_2_SEQUENCE=CAATGTCTTTCACGTATCAGGC
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=263,22
PRIMER_LEFT_2_TM=59.891
PRIMER_RIGHT_2_TM=59.637
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.4746
PRIMER_LEFT_3_PENALTY=0.466342
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=TGTTGAAGGACACAGTGATGGT
PRIMER_RIGHT_3_SEQUENCE=CAACTTGATCAACAGGGACAAA
PRIMER_LEFT_3=43,22
PRIMER_RIGHT_3=242,22
PRIMER_LEFT_3_TM=60.466
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=0.6096
PRIMER_LEFT_4_PENALTY=0.109200
PRIMER_RIGHT_4_PENALTY=0.500361
PRIMER_LEFT_4_SEQUENCE=ATGTTGAAGGACACAGTGATGG
PRIMER_RIGHT_4_SEQUENCE=GGGACAAAACGCAAAACTTCTA
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=228,22
PRIMER_LEFT_4_TM=59.891
PRIMER_RIGHT_4_TM=60.500
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00766 Ps_contig_mira-and-tgicl-ass_franssen_7371
SEQUENCE=GAAGGACACAAAGTTTCGCACAACTGAATTGAAACGCGCTTTTCACAACAAAACCAAAGCATTCAGGCTCAGCCCCTTCCTCCATTCCCCTTTTCTTCTTCTTCTCCGCGTTTCCGATCAGATCCTTATCACCCCCACCTTTCCAATCCACAAACACGATCGTGCTTCTTCTCTACGGTTTCGCCTTTTCCTTCATACACCAATTCTCGATTTCAATTTTCAATTTTCAATTATCATACATATATGTTAAATTAGGTAAAAAAAATATGTATAGCAATTTCAAGGAACAGGCGATAGAGTACGTGAAACAAGCCGTTCATGAAGATAATGCTGGTAACTATAGACGAAAGCGTTTCCTTTATATATGAACGCTTTG
TARGET=93,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8308
PRIMER_LEFT_PENALTY=0.761293
PRIMER_RIGHT_PENALTY=0.069526
PRIMER_LEFT_SEQUENCE=ACACAAAGTTTCGCACAACTGA
PRIMER_RIGHT_SEQUENCE=GATAAGGATCTGATCGGAAACG
PRIMER_LEFT=5,22
PRIMER_RIGHT=129,22
PRIMER_LEFT_TM=60.761
PRIMER_RIGHT_TM=59.930
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=125
PRIMER_PAIR_PENALTY_1=0.8567
PRIMER_LEFT_1_PENALTY=0.761293
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=ACACAAAGTTTCGCACAACTGA
PRIMER_RIGHT_1_SEQUENCE=TCTATCGCCTGTTCCTTGAAAT
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=297,22
PRIMER_LEFT_1_TM=60.761
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.9569
PRIMER_LEFT_2_PENALTY=0.761293
PRIMER_RIGHT_2_PENALTY=0.195574
PRIMER_LEFT_2_SEQUENCE=ACACAAAGTTTCGCACAACTGA
PRIMER_RIGHT_2_SEQUENCE=CGTACTCTATCGCCTGTTCCTT
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=302,22
PRIMER_LEFT_2_TM=60.761
PRIMER_RIGHT_2_TM=59.804
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=1.0233
PRIMER_LEFT_3_PENALTY=0.761293
PRIMER_RIGHT_3_PENALTY=0.261998
PRIMER_LEFT_3_SEQUENCE=ACACAAAGTTTCGCACAACTGA
PRIMER_RIGHT_3_SEQUENCE=ACTCTATCGCCTGTTCCTTGAA
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=299,22
PRIMER_LEFT_3_TM=60.761
PRIMER_RIGHT_3_TM=60.262
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=1.1335
PRIMER_LEFT_4_PENALTY=1.063952
PRIMER_RIGHT_4_PENALTY=0.069526
PRIMER_LEFT_4_SEQUENCE=CACAAAGTTTCGCACAACTGA
PRIMER_RIGHT_4_SEQUENCE=GATAAGGATCTGATCGGAAACG
PRIMER_LEFT_4=6,21
PRIMER_RIGHT_4=129,22
PRIMER_LEFT_4_TM=59.936
PRIMER_RIGHT_4_TM=59.930
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_00767 Ps_contig_mira-and-tgicl-ass_franssen_7374
SEQUENCE=CTTCGGTTGTCCACACTTCTTGCACTTCTCTCTTGATGCATAATTGTTGTTAGTGCAACCGGAGCAGATCCAATCGCCGATACGAGGGAGCCATTTGGAATCAGCGGGGGTTTTGTTATCAACAAGAAGACGTGGCTGTTTGCATCGATTGCAGAATGATCGGAAAGCGTAGTTTCGGTTGTTGCAACTGCTACACTCCCAATCACCTTCTCTACCTTCTCCCATTCTTCTTCTTCTTCTTCTCGACATTTAA
TARGET=225,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00768 Ps_contig_mira-and-tgicl-ass_franssen_7380
SEQUENCE=GATAAAAAATAAGGGAGGATKAATTAGCACTAGCTCGTCCCTTCTGGTTTTGTCTAAACGAAACCCCAACCGCCGGCGAACGACATCGTTTTCTGTTAACGAAACCCTAATCGAATAACGATACCGTTTTGATCAAAATGGGAACACCGGAAACTTCACGAGAGCCGTGTCCAGATCGCATCCTAGACGACATCGGAGGAGCCTTTGGAATGGGCGCCGTCGGCGGCTCCGCCTTCCACTTCCTCAAAGGTCTCTACAATTCCCCGAAAGGCGATCGATTAATCGGTGCAACACAAGCGGTTCGTCTCAACGCTCCAAGAATCGGTGGTAGTTTCGCTGTTTGGGGTGGTCTTTTCTCCTCTTTTGATTGTACTATGGTTTACGTTCGTCAGAAAGAAGATCCATGGAATTCGATTGTTGCTGGTGCTGCTACCGGTGGTTTTCTTGCTATGCGTCAGGGTTTTGCTGCTTCTGCTAGATCCGCTGCGTTTGGCGGTGTTCTTCTTGCGCTTATTGAAGGTGCCGGGATTGCGCTTAATAAGTTTCTTAGTGCTCAGCAGCCTATGCCGATTATGATGGATGAGATGCCGCCGCAGACTCCGGAGAATTCGGATTCCAACAAGTCGTGGTTTGGTGGAATCTTCGGTGGGGCCAAGGAAGAAGAAGAAGAATACTAGT
TARGET=657,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00769 Ps_contig_mira-and-tgicl-ass_franssen_7383
SEQUENCE=GAAGAAAAGGAAGGAAGAGGGAAGGTTGCAGTCGAGGAGACCGTGTCGTTGTGTCGGTCGTCGTCGTCAACAATACGAATAATTCAAGAACGACCAGTTTTCAGATCCACATATCTTCCCAAAACGTTTCAAATATGACGATGGGGGATGACGACTACACTGTAGATGATGCTCTCGTGACTCTGGGTTTTGGAAAATTTCAATTACTCGTTCTTGTTTATGCCGGCATTGGTTGGATTTTCTGAAGCTATGGAAATGATG
TARGET=57,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2507
PRIMER_LEFT_PENALTY=0.175636
PRIMER_RIGHT_PENALTY=0.075027
PRIMER_LEFT_SEQUENCE=GAAGAAAAGGAAGGAAGAGGGA
PRIMER_RIGHT_SEQUENCE=CGTTTTGGGAAGATATGTGGAT
PRIMER_LEFT=0,22
PRIMER_RIGHT=125,22
PRIMER_LEFT_TM=60.176
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=0.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=126
PRIMER_PAIR_PENALTY_1=0.2843
PRIMER_LEFT_1_PENALTY=0.175636
PRIMER_RIGHT_1_PENALTY=0.108684
PRIMER_LEFT_1_SEQUENCE=GAAGAAAAGGAAGGAAGAGGGA
PRIMER_RIGHT_1_SEQUENCE=TCTGAAAACTGGTCGTTCTTGA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=104,22
PRIMER_LEFT_1_TM=60.176
PRIMER_RIGHT_1_TM=59.891
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=0.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=0.2846
PRIMER_LEFT_2_PENALTY=0.175636
PRIMER_RIGHT_2_PENALTY=0.108929
PRIMER_LEFT_2_SEQUENCE=GAAGAAAAGGAAGGAAGAGGGA
PRIMER_RIGHT_2_SEQUENCE=AACGTTTTGGGAAGATATGTGG
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=127,22
PRIMER_LEFT_2_TM=60.176
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=0.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=128
PRIMER_PAIR_PENALTY_3=0.3593
PRIMER_LEFT_3_PENALTY=0.284310
PRIMER_RIGHT_3_PENALTY=0.075027
PRIMER_LEFT_3_SEQUENCE=AAGAAAAGGAAGGAAGAGGGAA
PRIMER_RIGHT_3_SEQUENCE=CGTTTTGGGAAGATATGTGGAT
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=125,22
PRIMER_LEFT_3_TM=59.716
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=0.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=0.3930
PRIMER_LEFT_4_PENALTY=0.284310
PRIMER_RIGHT_4_PENALTY=0.108684
PRIMER_LEFT_4_SEQUENCE=AAGAAAAGGAAGGAAGAGGGAA
PRIMER_RIGHT_4_SEQUENCE=TCTGAAAACTGGTCGTTCTTGA
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=104,22
PRIMER_LEFT_4_TM=59.716
PRIMER_RIGHT_4_TM=59.891
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=0.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=104
=
PRIMER_SEQUENCE_ID=SSR_00770 Ps_contig_mira-and-tgicl-ass_franssen_7392
SEQUENCE=ATCAACGCAGAGTACGCGGGAGTTTTGCAGAGGTTTTGGTGGGATAGGTGGGATCTTGCGTTATCAGCTTGATATGAGAACATTTGACGATTTTTCTGATGATGGAGGAGTTTATGACGATGAATAGCAATCAATGAAATACTTCCTACTACTGGTGCAGGAAAGGGAGGTCAAAATACCTGCACCAGTATGGGAGGTAGCTGCACGTGCTGCTGCTGCTCCTCCCGTTCCCCCTCTTCTGTCTGTGATGTTGCATGGAAAGTTTGACTGCAAAATGAGCTGCTTTACTCCGGAAGTGCTGTCTGATCTGAATTACTTCCTCACTTGTTTTCCCATTGTTCGATCGGCACTACAAACTTCAGCAACATTGCTGGCAACAGCTGGTGAAAACCTGTCATTTCCTACTCCTCTGCGATCTACTTGTATTCAGAAGGGCAAAAACAAAGTCATCCTCCCAAAAGAACACAACCTAGCATCAATGAAGTCTTGCGTGAACCAGTCTGTATATGTAATCTAGCTAAGCTTCGATGAAGAAGCAACCTGCTCACTAAGTTATCAAGGTGAACGAGATATTTCGTTGTATGCGTTTCTTCAAGGATAATCGAGCCTAGCATGCCACATCAATGCTTGTGTCTCCTACTATATATCATATGTGTGCCTCAGTCATGCTTTTGAAACTTTCGCTGCATTGAACTGTGTTCTTTTTTTWTTTATTGGTACTGTACTCATGGTTTGCTTCTATTGGAAGTATGGTTTGTATAATAGATTGCTTAAAGATGTTATATGTTATGAAAACTTCAGTTATTAAAATTAAATTATAAATTTACATC
TARGET=208,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00771 Ps_contig_mira-and-tgicl-ass_franssen_7400
SEQUENCE=GAATGAATTATTTGATGCAGCTATCTTCTCTTGGAGATGCTCATGATGAAATAGATTTTGAATTTCTTGGTAACTTAAGTGGTGATCCTTATATTGTTCATRCAAACATTTATGCACAAGGGAAAGGAAACAAAGAACAACAATTTTACCTGTGGTTTGATCCCACCAAGGACTTTCACACCTACTCCATTCTTTGGAATCCTCAAAGCATCATGTATATATTCTATTCTCTTCTATTATAAAGTTATATATTAGATGATATATTACTTATTTTCATATTTTAACCTTTTTGTGTGTGTGTGTAATATCATATATC
TARGET=291,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00772 Ps_contig_mira-and-tgicl-ass_franssen_7401
SEQUENCE=TAATCATCATCATCACTTTGTTTCCCTAATACTTTCTAAAGTAAGTATCCTTTTTGKTTATCGTCAAGATCGTCTCCTGTTCCTTCATCAGGATCTTCAGAGCCTTCTGAATCATCAGAATCTTCCTCATCATCATCCTCACCATCATCATCTTCACCAGAAGAATCTTCCTCATCTTTTCTCTCAAGAATCAAATCAATCAAACCCTTTTTAGTTATAGTTTCTTCATCAACAGAGAAAGAATCACCAAGGTCATCACCAGAGACAGATCGTTGTTTCTGTTTTGATGGCTTTTCTGAATCTTCATCATCTTCTTCACTT
TARGET=3,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00773 Ps_contig_mira-and-tgicl-ass_franssen_7423
SEQUENCE=GGGATTCCATAACCACCCAATTGAGAATACATTTTTCTGTCTTTGAACACTTTCTTTTCATACACGTAACATAACCATGTCTTCTGAGACGGTTGTCCTGAAAGTAAAGATGTCATGCACAGGCTGTTCAGGAGCGGTCAATAGAGTTTTGGAAAAAATGCAAGGTGTAGAATCATTTGAGATTGATATGAAGGAACAAAAGGTGACTGTGAAAGGAAATGTAAAGCCACAAGATGTTTTTGAAACTGTTTCCAAAACTGGAAAGAAAACTGAATTCTGGGTGGAGCCTGAAAACAAGTCTACAGAAACTGCAACTGAGGTTGAACATGTTATCAAACCTTCTGATTCAGAAGCTGCAGTTATTGCCTCTGTTGAGCTTGATAATAAGCCAATAGAAGCTTCAACTGAGGCTGAATCTGAAAATAAGTCCACAGAAACTGCAACCATTGGAGTTGTTGAGCCTAACAACAAGCTTTCTGAAACTGCCACTGTTGCTGCCTAAGAGATTACTACTTGTGTTTATTTCTCTTTGAAATTAGTCTATTTTAAATTTCAAAATGATCTTTGGTCGATGCTCATATGATATATAGATATATATATTCAGTGTGATGTGTTTGTGGTATCTAAGCTACAGATACTTTCTTACAATGCACTTAGGATGAATATTTTAATTGACTATACTAAGCAGTGTGTTATATATTAGAATTTAAATTAGTCGACT
TARGET=591,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.0465
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=3.039578
PRIMER_LEFT_SEQUENCE=TCAACTGAGGCTGAATCTGAAA
PRIMER_RIGHT_SEQUENCE=AGATACCACAAACACATCACACTG
PRIMER_LEFT=400,22
PRIMER_RIGHT=624,24
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=58.960
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=3.0574
PRIMER_LEFT_1_PENALTY=0.017846
PRIMER_RIGHT_1_PENALTY=3.039578
PRIMER_LEFT_1_SEQUENCE=TTGCCTCTGTTGAGCTTGATAA
PRIMER_RIGHT_1_SEQUENCE=AGATACCACAAACACATCACACTG
PRIMER_LEFT_1=362,22
PRIMER_RIGHT_1=624,24
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=58.960
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=3.0574
PRIMER_LEFT_2_PENALTY=0.017846
PRIMER_RIGHT_2_PENALTY=3.039578
PRIMER_LEFT_2_SEQUENCE=TTATTGCCTCTGTTGAGCTTGA
PRIMER_RIGHT_2_SEQUENCE=AGATACCACAAACACATCACACTG
PRIMER_LEFT_2=359,22
PRIMER_RIGHT_2=624,24
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=58.960
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=3.0651
PRIMER_LEFT_3_PENALTY=0.025566
PRIMER_RIGHT_3_PENALTY=3.039578
PRIMER_LEFT_3_SEQUENCE=GAAGCTTCAACTGAGGCTGAAT
PRIMER_RIGHT_3_SEQUENCE=AGATACCACAAACACATCACACTG
PRIMER_LEFT_3=394,22
PRIMER_RIGHT_3=624,24
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=58.960
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=231
PRIMER_PAIR_PENALTY_4=3.0809
PRIMER_LEFT_4_PENALTY=0.006945
PRIMER_RIGHT_4_PENALTY=3.073969
PRIMER_LEFT_4_SEQUENCE=TCAACTGAGGCTGAATCTGAAA
PRIMER_RIGHT_4_SEQUENCE=GATACCACAAACACATCACACTG
PRIMER_LEFT_4=400,22
PRIMER_RIGHT_4=623,23
PRIMER_LEFT_4_TM=59.993
PRIMER_RIGHT_4_TM=57.926
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_00774 Ps_contig_mira-and-tgicl-ass_franssen_7424
SEQUENCE=TATTTTTTTTTAACGACCAAAGTAAAAATAGTGACAATTTAAATTCTAATATATAAAGCACACTGCTTAGTATAGTCAAATTAAAATATTCATCCTAAGTGCATTGTAAGAAAGTATCTGTAGCTTAGATACCACAAACACATCACACTGAATATATATATCTATATATCATATGAGCATCGACCAAAGNTCATTTTAGAAAATTTAAAATAGACTTAATTTCAAAAGAGAAAATAAACACAAGTATAT
TARGET=152,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00775 Ps_contig_mira-and-tgicl-ass_franssen_7429
SEQUENCE=AAATTCGATGTTGTTGTTGTCAGTTGTTGCTCACGCTCAGCCAAAAAACTAGCCAATGACAAATGCCGAAAAGCATCAAAACGTGTAGCATTTTATACCATTGACTGTAGGGATTCTTCTGGTGAAATTTTCGTTGATTTGCAGGACTATAAATATTCTAAGAAAAAAATGGAGGAAACTATTGAATGCCACCTAAAATATCCAAGTTTTGAGGATGCTTTATCAGTACCTTGGAGGGCACTTCACAGGAGAATGTCAAAGCTGTACTTTGCAATGAGAGTAATAGAGAAGTTTGAAGAGGCTGAAGGACGCAGTGCGGGAGATGTTTCGGAGGCAGATCTTTCCGATGTTCTCAAGCTTAAAAAGGAACTTTGTACTGCACAATCTCTCAATGAATCTCATGTTCCCGACACTCTTCTAGAAAGATTGGTAGCGGATACAGCCGAGTTTCCTCCCGTCTGTGCTGTTATTGGGGGAATCCTTGGACAGGAGGTTATCAAAGCTATTTCTGGAAAAGGGGACCCCATAAAAAACTTCTTCTATTTTGACGCGTCTGATGGAAAGGGTGTTATAGAAGACATATCTGATTCAAACGCTGGAAAGTAGCTTCTCTGGGTCAGTGTTTCTAATGAGTCACCCGCAATCGTGGTTGTGAATATTGAACCTTTTGCTGCATTAACTGCTCATTTATAGTTTAGTAGTTCCTTATTTGTTTTAGTCATTGGCAATTTAGGATCAGAAATGTTTTGAAATGTAGACACTCGAATGTATTTATGCTGACCTTCAAAGCCATCTTCGAAGTTGCAAAGCTTCATGATGGTTAAGGAAAAAAGCTCAACGTTGTTTATATACTGGATTCTTTAGTGGGTAAACAAGGTTAATTGAACTTCTATAGTAATGATTTTTGCTATAGTTGACCTGATGACATCTWWSYA
TARGET=9,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00776 Ps_contig_mira-and-tgicl-ass_franssen_7431
SEQUENCE=CATACTTCGTTCAAACTAAAACATGGATTCATCAAACACTAATCTTCCGGTGCTGTCTCTGCCTAAAGCAGACTCGGAGCTTGATGCTGCTGCAATCAAAATTCAAAAGGTTTATAAGAGTTACCAAACAWGAAAAAACCTTGCAGATTGTGCAATTATTGTGGAAGAGTTATGGTGGAGGGCATTGGAGTTTGCTGCTCTTAAAAGAGCCTCCGCTTCGTTTGTTGATGGTGAGAAATCTAAACAAGTTCGCGTCTCTGAGTTTGTATGCTGCTGCAAACTAAAAATACAAAAGGAATTACAAAGGTTACCGTACTAGAAGAACATTGAAGAATGGAAGAAAGTTAAAGAAAAAAATAATCGTTTGTGTCATATGAATGAACGATTGTGATATATCTTATTATGTATTTATGTTATATATTTATTTATTTTGTTTTGATCATTTTTTAAAATAAAATAATGTTGGAAATAAAGTCTTTTGTGTTTAGGAAAAATATGCGTGAGAATATTAGAGGTTTG
TARGET=419,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00777 Ps_contig_mira-and-tgicl-ass_franssen_7432
SEQUENCE=CCTATCCCCTGTTCGTGTCKCAGGACAGTCACTTCAAAGTTTTCTTGTAGAAGAGAAGGATGAGGGTACGTGGCAAAGTACCTTATACCTCTTCATCATCAAAAGGAACTAACAACATTGACAACAACTTCAACACAGCATTGGTTTCCCTTCCTACTTGAAGACAGATATGTTATGTTTATTGTTTTCTACAACAAATATTATAGCTAATTAATTTATTATTATTATTTATTATTATTATTTATTTGTTTTGTTAACTTTAAGTTGTT
TARGET=216,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00778 Ps_contig_mira-and-tgicl-ass_franssen_7432
SEQUENCE=CCTATCCCCTGTTCGTGTCKCAGGACAGTCACTTCAAAGTTTTCTTGTAGAAGAGAAGGATGAGGGTACGTGGCAAAGTACCTTATACCTCTTCATCATCAAAAGGAACTAACAACATTGACAACAACTTCAACACAGCATTGGTTTCCCTTCCTACTTGAAGACAGATATGTTATGTTTATTGTTTTCTACAACAAATATTATAGCTAATTAATTTATTATTATTATTTATTATTATTATTTATTTGTTTTGTTAACTTTAAGTTGTT
TARGET=229,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00779 Ps_contig_mira-and-tgicl-ass_franssen_7442
SEQUENCE=AAGGAAGGCTTTTGAATAGGAAATCAATGGGAGAAGATCTTTTTTGGACTATARGAGGTGGTGGTGGTGCTAGTTTTGGTGTTGTTTTATCTTATACKGTTAAATTAGTATCGGTTCCTGAAACTGTTACTGTTTTCCGTATTGAGAAAACTTTAGATCAAAATGCTA
TARGET=57,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00780 Ps_contig_mira-and-tgicl-ass_franssen_7444
SEQUENCE=AAGAATAACACCAAAACTAGCACCACCACCACCTCTTATAGCCCAAAAGAGATCTTCTCCATAAGTCTGAGCATAACCTTCTCTGATAGCCTTCCTCTGACTACCATTCCTATGACCCAAAATCGATATAATCAAATCTTCATTGGTCCCCCAACCTACAATCAAAAAATCAAAATCGATCCATTAACAACACCCGATCCTTAAAC
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00781 Ps_contig_mira-and-tgicl-ass_franssen_7446
SEQUENCE=CTTCTTTCTTTTCTGTTTTTTNTTTCCTCCAAAGTTTACTTKATTAAAGTACTGAAARTTTAAACCTAAACTTTTAGTCAGTACATAGATTCTTTTTCCTTTTTTCATCTTAGAAGTGATTAAAATAAGCACTTATGCTGCTTAGAAACAGATCAAGAGCTGTAACCAAACAAAGTCTAATGGGTGAACAACAATCACCAAGTCAAAACTTCACAAACACAATCCCTTCTCTATTTGGTTCATCAAACTATAGAGACTTTCATTTCACTTCCAAAAAAGCTGAATCTTTGATAAGTCCAACATCGATTCTTGACACAAAAGCTTTATCTCATTTTGAGAATCCTTTCTCAGTTAACAATAGGAACAACAACAACAACAACAAAGTTTGTAGCTCAAGGGACAAACTAGATTCGAAAAACGTAGGACTTGGTCTTGTTGGTGTTCTAAAAGATGAACAGCCTTTTCATCAAAATTCTGAAAAAATAAGGAATAGAAAAGTTCTGTTTGGAACTGATCAGCTTAGAGTGAAAATCCCTCCTCTACCAACTTCCACGTTTGAGTCAAAAAGTGATTTTGGTAGCAAAATGAATTATTCTTCATCATCAGAAAAAGGTGTTCTTAGTTTGAGTGAAATGGAGGTTTATGAAGAATACACATGTGTGATATCTCATGGAGCAAATCCAAAAACAACACATATATTCGATAATTGTGTTGTGGGAAGTTACTGTTCTGTCCCTAATAGTCCTCACTCTTTTTCACTTGATTTTCTTAGCTTTTGTTACACTTGCAAAATTCATCTTCAGCATACAAAAGACATCTTCATTTACAGAGGAGAAAAAGCTTTTTGCAGTCAAGAATGTCGTAACCAAGAGATGATGTTAGATGAAGGAGAAAATTAAGAAATTAATTTTGCGTTTTTATATATATTTTTTTATGTTCTATTTGTGTGATTTTTTCTTGTTGTATCCTGCAAATAGAGATAAAAGAGGATGTATTATTGTAGAGTTCATTTCAAGAAATAAGTACTAATATTACTATTGAATTGCTTTTGATTATGATGATGATGATATGAAATT
TARGET=363,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00782 Ps_contig_mira-and-tgicl-ass_franssen_7446
SEQUENCE=CTTCTTTCTTTTCTGTTTTTTNTTTCCTCCAAAGTTTACTTKATTAAAGTACTGAAARTTTAAACCTAAACTTTTAGTCAGTACATAGATTCTTTTTCCTTTTTTCATCTTAGAAGTGATTAAAATAAGCACTTATGCTGCTTAGAAACAGATCAAGAGCTGTAACCAAACAAAGTCTAATGGGTGAACAACAATCACCAAGTCAAAACTTCACAAACACAATCCCTTCTCTATTTGGTTCATCAAACTATAGAGACTTTCATTTCACTTCCAAAAAAGCTGAATCTTTGATAAGTCCAACATCGATTCTTGACACAAAAGCTTTATCTCATTTTGAGAATCCTTTCTCAGTTAACAATAGGAACAACAACAACAACAACAAAGTTTGTAGCTCAAGGGACAAACTAGATTCGAAAAACGTAGGACTTGGTCTTGTTGGTGTTCTAAAAGATGAACAGCCTTTTCATCAAAATTCTGAAAAAATAAGGAATAGAAAAGTTCTGTTTGGAACTGATCAGCTTAGAGTGAAAATCCCTCCTCTACCAACTTCCACGTTTGAGTCAAAAAGTGATTTTGGTAGCAAAATGAATTATTCTTCATCATCAGAAAAAGGTGTTCTTAGTTTGAGTGAAATGGAGGTTTATGAAGAATACACATGTGTGATATCTCATGGAGCAAATCCAAAAACAACACATATATTCGATAATTGTGTTGTGGGAAGTTACTGTTCTGTCCCTAATAGTCCTCACTCTTTTTCACTTGATTTTCTTAGCTTTTGTTACACTTGCAAAATTCATCTTCAGCATACAAAAGACATCTTCATTTACAGAGGAGAAAAAGCTTTTTGCAGTCAAGAATGTCGTAACCAAGAGATGATGTTAGATGAAGGAGAAAATTAAGAAATTAATTTTGCGTTTTTATATATATTTTTTTATGTTCTATTTGTGTGATTTTTTCTTGTTGTATCCTGCAAATAGAGATAAAAGAGGATGTATTATTGTAGAGTTCATTTCAAGAAATAAGTACTAATATTACTATTGAATTGCTTTTGATTATGATGATGATGATATGAAATT
TARGET=1055,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00783 Ps_contig_mira-and-tgicl-ass_franssen_7451
SEQUENCE=AAGACAGAATGATTATTGCAAGGGTTTTGGTAGTTWRATWRKGRAATACTGTGGACAAGAAAAACAAAAGGACTACATTGAGATATGTGAAATGGAAAGTGTATTGAAGTCAAGTTTGTTAAAGGAACATTCTCCAGTAATGAAATTAAAAAGAAATGATTATTCAAGAGGAGAGTGAGGAATATTGTAAGTAAGTAACTAA
TARGET=187,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00784 Ps_contig_mira-and-tgicl-ass_franssen_7453
SEQUENCE=TTTWTGGACCAAGCTATCTGGTTTATTTCCATAAGAAAATTTATTATAAATATCACAATTCAAAACATACAAATTCAATGTAGCCTTTTTAAAAACTTAACATAGCTTAATCAAATTTGACAACAACAAAAAAAAGATCTCCCTTCTATATTACAGAAGATTTCTTCAGCATAAGAATTTACTTATTTGTATCACAATCTTCTAAATAGTGATTGTCTAGTCTCTATCGATCGGCAACTCGGACGCATCATGCAGAAAGTGTAGATAACAATTTGTCCAAACCAACATACAAGCTATCACAAAGAATAATCAGACTAAAATATGAACACAACACTGTCATGAACTCTGGACTTTTGATTCTGAGCCACACATTCTGAAATCCACATAAGATTCCTGATCTCCTGCTCCCTTAACCTCATATATGCGAAGAACACAGCATAGTGAAACTGTTGCTCAAATGCTAAGCAATGCCTCTTGACCTCTTCCTCGTAGAATGCCTTGTCAAGCATCTGGCTTTCTCCATATGACAGCTTGGCAAAAATAGATTGATAAGGAGGGTATTTTTCCATGACAGCACGAACCTGATCAATGTCCTCACAGACGGCAAGTTCCTCATGACCATATGGGTAGAACAAACCGAAGTTAGAGTACAGTTTCCTACGGTCATCTCTGGTAAGCTCAGTACCAATGCTGTTAATGGTAATATTCACAGCCCTTCTATCAGCCTCAAAAGCTAGAAGGTCAGACATTATTTCAGCAGTGGCACCACCAAGTTTCTGACAAAATCTGTAAAAATCTTCAAGGTATGCCTTGTAAAGAGTGTTCCTCATTATTTCAATGTTCATGTCATCCAAGTCCTCTGATGTAATGCACTCAGAGAAGTATGGAGCAAGAGGGGTATCAACAAGAACCAGCCTGTAAAGCTCCCGCATATTCTGAGCAACTGCCAGAGTAGCAATACTGTCAAACATGCCCAGTGGATGGCATTTTTCAAGAAGTTCTTGGACATCTCTCTCATGCAAGGTGCCAGTAACAATCAAAACAACATTGTCTATCATGTGCCCATATGTGATATATTCTAAAAAGGTCGACAAGGGTTCTGTAGCTTGGCATAGCATGTGCTTGTAATCATCAACCAGCTTAAGAGTACATTTCTCCACAATTGTTGTGGTATGCAACGGAGAAGGTTCGTTTTGGAGATAAGGACCGTACTCGGTAGCGGAGAGATGCATCTTGATATCATCGAGGGTTTCGCATTGGCAGAGATTGTTGTAATCGGAGGTGGTGAGTAGACCGGAACGGTGACCGCGGACGATTGCCTCAAGGTAACCGCCGTGGATGTTGAAGGTGAGAGCTTCGAAACCGTACATATTGAATTGGATCTGGATTCGGGTCGGGTTAACGAATGGATCGAGAATTGGAGAGAGAAAGAGAGAAGCACCTCCTTCAGAGAAAATCTGAACCGAGAAGAAGAAGAAAGTGCCTSGTTGTCC
TARGET=1466,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00785 Ps_contig_mira-and-tgicl-ass_franssen_7465
SEQUENCE=CGGGGGGTAGTTAATTAGAGAGAGACTGTGTGAAMGRGGGATAGCAACCAACAGCTAGAAAGAGAGAATAAGAGAGAGAAAGAAGAGAGAGAGAAAGAAATTCCAGATGTCTTTGGCAATGGGGAAAATTGGAAATCAAGATGTGCAACAAGAAGAACAGAGGCAGAACGACGGTGAACGGTTGTTTTTCACGGTGGTGCTGAGATTATCAGTTCAGTNTTATCATTCTTCTTTTNAGTTGCGTNTTTGTTCNGAT
TARGET=84,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00786 Ps_contig_mira-and-tgicl-ass_franssen_7467
SEQUENCE=GTATGCCGGGTCCCGTTGATACTATCAATGTAGTATTTTTCTCTATATGATCATGAGAATGAAGTTATGATAGGAGTAGATGATATTTTTAGTTTCAAAATGCATAGTTGTGTTTTGAAAAAATGTAAATGATCATTCTGAGGACACCTATTTTTGTTTGTTTGCAAACTNTTTTCTTAAATTCACTCCCCTAATCCTTGAGAATTAGTACAAAAGTAAACTTCTTAATTGTTATGAATAACAATTAAATTGTTAATAAAATTAAATTAGTTAATTTTAAAAGGTTTAAAAAAAAAGTTTTTTT
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00787 Ps_contig_mira-and-tgicl-ass_franssen_7474
SEQUENCE=CTAACTGCAAATTCATWGCACTCACCAGATCAGAACCCTTCAAAATTCTTGAAGAGTAGGTGCAGCTTCTTCTGTTCTAGTTAGGAACTTGCAGATACTACTCATCTAGAAATTTTCTAGCTTTGTATACAGCATGGCAAGGACCAATCCTATGTTATCTATGTTGTTCATTTCACTTATATATATATAAATATCTAGCCTAGTATATAATCATATATATAYCATCATGTCGTAAAG
TARGET=178,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00788 Ps_contig_mira-and-tgicl-ass_franssen_7477
SEQUENCE=GTCGSGGGATCCAAACACAAAAATTCTAATAGCAATATTTAAGTAAGATTATAAGAATGTCATCTCGTGTGCTCAAGAAACTCATCGCCATGCTTGAGGCTGAAGAAGCCGAAGCTGCGAAATCCAACAACCATGGTACTGCTGACGCATTTAACAACCATGGCGGCGGGAACCAAAATTTCTCCGGTGCCAAAATCAACAGCGGTTCAAATTCTGGGGACCGAGAGAAACACCGCACCACCAACAATCATGGCGAGCGTACCGTAAATAACAGTGGTACTTTCCATGGTAATGGCAATGGAGGGTACACTGAGGGTAACTTTGACGCATCCACAACGAACTTTAACAAATAGTTATAAAGTATTTGTGATGATAAAAATGGTATATGCAAGTAATAATGAAGTTGAAGATATATATATTTTCGATGGTTTATGGTTGTATGTTGCTCTATAGGAAATTATAAATAAACGCACTAGTGTGCTATGATAAATCTTCTTATGTTTGATTATATATAGTTACTATACAGGTTATAATAAACGATAATTTATCTTAACTTTAAT
TARGET=410,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00789 Ps_contig_mira-and-tgicl-ass_franssen_7499
SEQUENCE=CGGGGATATRAAAAATAGATAAATAAATAAAATAGTGAGTTAAAGATCTATAACCCTTCTTTTCACGAGGGATCAAAATCCATAATTGAAGCATACAATCAACAAAACCAGAACCCATTATTTCTGTGAAAGAAAAAAGCAAACTATTTTTTCTACTACAGTATCATGGGTATTGTTGTGGCTATTGCTGTGTCATCGCCTATTTCCATTCTACTATTACCTCTGGAATTCGGCCTCAATCAGT
TARGET=19,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00790 Ps_contig_mira-and-tgicl-ass_franssen_7500
SEQUENCE=GTCGGTAGTAGTATTAAATTAAATTAAAATTAGTGAGTTAAAGATCTATAACCCTTCTTTTCACGAGGGATCAAAATCCATAATTGAAGCATACAATCAACAAAACCAGAACCCATTATTTCTGTGAAGAAAAAAGCAAACATTTTTTCTACTACAGTATGATGGGTATTCTTGTGGCCATTGCTGTGTTATCGCCATTTCCATTCTACTATTACCTCTGGAATTGGCCTCAATCATGGGTAGACTTATGCGGTAAAGAAAGAGATCCTTCAAAGATTATGGCTTACGTTTCTCATTTCTTGAAGCTGATTCAGTTCATTGCGCTCTTTTCTGTCTCTTCTTTTCATTGGCCTCCTTTTTACTTCTGGCCCCTTTTTGCATTTGGACAGTTTCTTAACTTCAGGGTTTATCAGCTGCTCGGTGAAGCCGGGACATACTATGGCGTACGCTTTGGAAAAACGATTCCGTGGGTAACCGAATTCCCTTTCGGGGTCATCAGTGATCCTCAATATGTTGGCAGCATCTTGAGTCTTGTTGCATGCCTTTCATGGGTTCCTTATCAGTACATTCTCCTGTGGATTTTAGGGTACGTGTTTATGATTCGCGTTGAATCAAAGGAAGATCAATCTACTCGTGCGAAGCCGCTCAATTGATTTGATTTAAGTAGTTAGATATAGCTTAGAGGATTCTGTAGCATCTAAAGTTGAGAAGGTGTTGTTAAATAATGGAACTCTCTCTCTGTCTTTCTATCTAGCTAGTTGTTCTTCAAATTCAATGATGGTGGATTTGAATTTGGTGTTCTTGTGTTTTGTTTAGGTCATGTGTGAATGTCTGAATTGTAATAAGA
TARGET=13,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00791 Ps_contig_mira-and-tgicl-ass_franssen_7500
SEQUENCE=GTCGGTAGTAGTATTAAATTAAATTAAAATTAGTGAGTTAAAGATCTATAACCCTTCTTTTCACGAGGGATCAAAATCCATAATTGAAGCATACAATCAACAAAACCAGAACCCATTATTTCTGTGAAGAAAAAAGCAAACATTTTTTCTACTACAGTATGATGGGTATTCTTGTGGCCATTGCTGTGTTATCGCCATTTCCATTCTACTATTACCTCTGGAATTGGCCTCAATCATGGGTAGACTTATGCGGTAAAGAAAGAGATCCTTCAAAGATTATGGCTTACGTTTCTCATTTCTTGAAGCTGATTCAGTTCATTGCGCTCTTTTCTGTCTCTTCTTTTCATTGGCCTCCTTTTTACTTCTGGCCCCTTTTTGCATTTGGACAGTTTCTTAACTTCAGGGTTTATCAGCTGCTCGGTGAAGCCGGGACATACTATGGCGTACGCTTTGGAAAAACGATTCCGTGGGTAACCGAATTCCCTTTCGGGGTCATCAGTGATCCTCAATATGTTGGCAGCATCTTGAGTCTTGTTGCATGCCTTTCATGGGTTCCTTATCAGTACATTCTCCTGTGGATTTTAGGGTACGTGTTTATGATTCGCGTTGAATCAAAGGAAGATCAATCTACTCGTGCGAAGCCGCTCAATTGATTTGATTTAAGTAGTTAGATATAGCTTAGAGGATTCTGTAGCATCTAAAGTTGAGAAGGTGTTGTTAAATAATGGAACTCTCTCTCTGTCTTTCTATCTAGCTAGTTGTTCTTCAAATTCAATGATGGTGGATTTGAATTTGGTGTTCTTGTGTTTTGTTTAGGTCATGTGTGAATGTCTGAATTGTAATAAGA
TARGET=731,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5650
PRIMER_LEFT_PENALTY=0.113803
PRIMER_RIGHT_PENALTY=0.451213
PRIMER_LEFT_SEQUENCE=CTCCTGTGGATTTTAGGGTACG
PRIMER_RIGHT_SEQUENCE=GAACACCAAATTCAAATCCACC
PRIMER_LEFT=569,22
PRIMER_RIGHT=800,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=60.451
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.7150
PRIMER_LEFT_1_PENALTY=0.263824
PRIMER_RIGHT_1_PENALTY=0.451213
PRIMER_LEFT_1_SEQUENCE=AGGGTACGTGTTTATGATTCGC
PRIMER_RIGHT_1_SEQUENCE=GAACACCAAATTCAAATCCACC
PRIMER_LEFT_1=583,22
PRIMER_RIGHT_1=800,22
PRIMER_LEFT_1_TM=60.264
PRIMER_RIGHT_1_TM=60.451
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=218
PRIMER_PAIR_PENALTY_2=0.7295
PRIMER_LEFT_2_PENALTY=0.278329
PRIMER_RIGHT_2_PENALTY=0.451213
PRIMER_LEFT_2_SEQUENCE=TATGTTGGCAGCATCTTGAGTC
PRIMER_RIGHT_2_SEQUENCE=GAACACCAAATTCAAATCCACC
PRIMER_LEFT_2=509,22
PRIMER_RIGHT_2=800,22
PRIMER_LEFT_2_TM=60.278
PRIMER_RIGHT_2_TM=60.451
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=292
PRIMER_PAIR_PENALTY_3=1.3443
PRIMER_LEFT_3_PENALTY=0.893116
PRIMER_RIGHT_3_PENALTY=0.451213
PRIMER_LEFT_3_SEQUENCE=ATGCCTTTCATGGGTTCCTTAT
PRIMER_RIGHT_3_SEQUENCE=GAACACCAAATTCAAATCCACC
PRIMER_LEFT_3=538,22
PRIMER_RIGHT_3=800,22
PRIMER_LEFT_3_TM=60.893
PRIMER_RIGHT_3_TM=60.451
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=1.3573
PRIMER_LEFT_4_PENALTY=0.906133
PRIMER_RIGHT_4_PENALTY=0.451213
PRIMER_LEFT_4_SEQUENCE=TACGTGTTTATGATTCGCGTTG
PRIMER_RIGHT_4_SEQUENCE=GAACACCAAATTCAAATCCACC
PRIMER_LEFT_4=587,22
PRIMER_RIGHT_4=800,22
PRIMER_LEFT_4_TM=60.906
PRIMER_RIGHT_4_TM=60.451
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_00792 Ps_contig_mira-and-tgicl-ass_franssen_7509
SEQUENCE=GTCGGSGGATATCGATCTATTAACATAATAAAATTAGTGTTCGTAAGATAAGATCATGGTTATTGTTTCAACACCACCGGAAGACGAAAACCGAAAGCATGATGGCTGCTTCATCATCATCATCATCAACATCAAGCATCATAACCATGCATCATGACATCATCCATACACACATCCTCACACGCCTTGACGCCGCCRCGCTGGCAACAACCGCTTCCACTTGCTCGCACCTCCGCGACCTCTGCATAGACGACAGTCTYTGGCGAAAAATCTGCACCGCCACGTGGCCTTCCTTAGTAGACCCCACCACCTGCCACATCATCTCCACTTTCCCAAACCGCCACCGTTCGCTCTTCTCCGACGCGTTCCCCGCACTTCATCATTTCTCTTGCAATTCAAATCGTCCGTCGTCTCCTCCTCCGGCAGAGATCATTTCCGCCGTGGATATTTTCTACAAAGGTAAGCCGGTGTTTTCGAGAGTTCGGCGAACCGAAACAACGAAGAACTCATTTCTATTCTCGCCGCTGTGGATTGAAATTCTGGAACCGAACGAGTTAATTCCGACGCCGGTGAAGTTCGTAAGAAAAGACGAAGAATTAATGAAGCATTTGGAAGAGAATTTATGTTTAAGCTGGATCGTAATCGATCCAACTGGAAAACGATCGGCGAATGTTTCGAGTCGGAAGCCGGTTTTAGTACGGCGGCACTGGCTCACAAGAGACGTGGAGATTCTATTTGCAGTGACGATGGCGGGGGAGCCGCGACGCACGACGGAGAGTGTACAGTGTATGGTGAAGGTCACGTGCTGTGGGAAAGTAGGAGGGGAGTTGCACGTGAGGGAGGTGAATTTAGTGATGGAGGATATGGAAGGAGGGAAGGTGAGTGGGAAAGAAGGGGTTATAATTTTGCAGAAGGCGATGGAGTTTGGGGAGAGGAAGAAAGTGGGTGAAGTGGGAGAGATGACGGAGAGGTTTGATAGATTTTTGGGTATGATTAGGGAGAGGAGAGAGAGGAGACATAGAAGACAGAGGGAACGTGATGGTGTTTCTATGGTGGTTGCGTTCATTGTTTGCATTTGGTTATGTTACCTAGCTGGTTTTTGAGGGAGGCSTTGACTTTTACTACAATATACAGTGATTGTG
TARGET=111,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00793 Ps_contig_mira-and-tgicl-ass_franssen_7510
SEQUENCE=CGGGGATATCGATCTATTAACATAATAAAATTAGTGTTCGTAAGATAAGATCATGGTTATTGTTTCAACACCACCGGAAGCAAAAACCGAAAGCATGRTGGCTGCTTCATCATCATCATCAACATCGAGCATCATAACCATGCATCATGACATCATCCATACCACACATCCTCACACGCCTTGACSSCCGCCACGCTGGCAACAACCCGCTTCCACTTGCTCG
TARGET=107,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00794 Ps_contig_mira-and-tgicl-ass_franssen_7540
SEQUENCE=TATATATGAGTTGTAGGATGAGGATATTGATAAAATTWGWAAAAAAAGTTTTTTTAAAGTTTTTAGTTTTGTAGTTAAAAAGTTAGAATNTATATTATTATATCGYTATATGTGATGATGATGAACATGATGATATGTAATGTTTGGGGTTTTATGCTGTACTCTATTATGGGATATCACAGTGATGTTGATATCGTGAATTGAATTTCAATGCACAAGAATAATTAATAGCTTGGTATTAGCTTAGTTGATTTGTTGTTCTGTTAAG
TARGET=113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00795 Ps_contig_mira-and-tgicl-ass_franssen_7541
SEQUENCE=ACAACAAATCAACKWAGCTARTACCAAGCTATTAATTATTCTTGTGCATTGAAATTCAATTCACGATATCAACATCACTGTGATATCCCATAATAGAGTACAGCAACTAAAAACCCCAAACATTACATATCATCATGTTCATCATCATCACATATAACGATAGTAATAATAATTAGAATTCTAACTTTTTAACTACAAAACTAAAACTTAAAAAAAACTTTTTTTTCAAATTTTATACAAATTACTCCTCATCCTACAAACTCATATAA
TARGET=139,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00796 Ps_contig_mira-and-tgicl-ass_franssen_7542
SEQUENCE=TTTMYAAGTCTTAATTTAATTATTCTTGTGCATTGAAATTCAATTCACGATATCAACATCACTGTGATACTCCCATAATAGAGTACAGCAACTAAAAMCCCCAANATTACATATCATCATGTTCATCATCATCACATATAACGATAGTAATAATATANATTCTAACTTTTAACTACAAAACTAAAACTTTAAAAAAACTTTTTTTTCAAATTTTATACAAATTACTCCCTACTACTTCCCTAACCAAAACTTCGTAAA
TARGET=123,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00797 Ps_contig_mira-and-tgicl-ass_franssen_7561
SEQUENCE=CTTAGTCTGTAGTATAGTTTGTTTACTATTAAATTTAACAAAATAATTTCTTTGTCTTCAACACATGACAAAACAATCTTATATGCTAATATGAGATTACTACCTTTTTTTTTGGTCCAAGGTTGCAAAACAGCTTAAACAAACAGAATAAGTCCCAATAACCTAACTGAACTATTCAAGAGACACTGATATGTACTACACAAGGCTCAACCTTCAACTATTACCTGTATTTTGAACTTACACTTTTATCTGGTCTCCATATCCTTCTTGGAGACGAAATTTTGTTGGGGGACGACGTTTTCGTTGTTTCCAATTGAACTTCCAACTTTGACACATCTAAGGATAACTTTTTCATAATGAAACGATCCAAATCAAACTCACTTTTATCACCCTCGTTGGTATTATCATCATCCTCACTAGAATAATCAACCATGTTTTTCTTCGTTCCGGTAATATTTTTCATGGAATCTGGTGACACTTGGAATGAATTTGGGTCCATCTTCAAGAGTCTCTTCAAACTGTCAACGGCAGGGTCTAAAATATCTAAGAATCCCGTAACCCATGAAGCATCATCTACAACTTCTGTGCGCTGCTGGAAATAAAGGTCACGAATCTCGTCAGCGCGCTCGGGATTTCCTTGATCTTCCTCCAGTGATGCCCAAGTCATCCATGTCACATAACTCTGAGAACTTACATTCAACGAGGATCTAAATAATCTCCGAGCATTTGAAAGATTGCCAACCCTCTGCTCCAGAACACCCCATGCCTGGAGGCACCTGGCCGCAGATTCAGTGTTTAGATTAATTGATAAAACTTTTTGGTATAATTGTCTTGCTGTATTCAAGTTTCCTTCCTTCCATTCCATCCAACCCCAAGCCAACCAAACAGGTTGATGCTTCGGATCTATTTCAGATGCTCTCTTGAACAAAACACGAGCACGGTTTGCACTTGAGTGTTTGTATTCTATCAATGCAAGAGACTGAAGGAAGAAACGGMAYMMMTAACCCTTAAGGAAATTTAAAAGAGCGTGGCCTATCTTTAGAAGTTTTCTTCCCT
TARGET=847,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00798 Ps_contig_mira-and-tgicl-ass_franssen_7564
SEQUENCE=TAACAAGAATTTCTTCCAAAATTACCAAGATGGCTTAAAAGCTTTTTCTGATAGCCCCATCATTGGAGAGATACGGGGAGCTGGCTTGATCATGGGGACCGAGTTCACAGATAACAAATCACCTAATGATCCATTTCCTCCCGAATGGGGAATAGGTGCCTATTTCGGAGCACAATGTGAGAAACATGGCATGTTAGTTCGTGTAGCCGGAGATAATATCATGATATGTCCACCGCTTATCATCTCTTCTGAAGAAGTTGATGAGTTAATCAGCATTTATGGGAAAGCTTTAAGAGAAACGGAAAAGAGAGTCCAGGAGCTCAAGTCTCAACGCAAGTAACCTAGGCCGATGATGATGAAACAATAAACTCTGGCCCCTTCGTGTGAAAAGGAATTATTTATTGTATAATAACTTACTGTAAGTATTACTGTGTGTATGTGCGCTTAATTGGGATCCCTATTAAAGTAAATTGCAGAGGGTACAGTTCCAATTTTAAGCATTAAAGTTTATTAAATTTAATTTCAAAGTACAAATGTTAAATAAATCAAGGGAAGTTCATWTATAGHAGMWMAATTGGTATAATTGAGATATTTTATTTATTTATTTTTAAAAGATGAAATTTCAACTATTGGGTTAATTAGT
TARGET=593,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00799 Ps_contig_mira-and-tgicl-ass_franssen_7574
SEQUENCE=AGTAATATTTTTCTTCAGTCGGCATCATCTTTGCATTTTTTAGTGAGTTAAGGGCAAGTAATACAACCCCTATAAGGCCTTGTACATATTCAGAAGGTTTAGAAATTCCTGAAGATCCCCACTTACCATTAGCAGGAAGTTTATTATTATTACCCATTGCTGCCGACGATGTAGGTTGATATTCTGGTGGACCATGTGGATAGCCTTGCGTGCTACTGCTATGCACCATATGTCCATTTTGTTCATTCGTTGATCTTACCAGAAGTGGAGTACCCTTAGGATTAGGTTTCAACTCTCCACTCCGTTGCGGAACAGTACGGGGGTTACGAGCATTATTAGG
TARGET=141,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1413
PRIMER_LEFT_PENALTY=0.014720
PRIMER_RIGHT_PENALTY=0.126537
PRIMER_LEFT_SEQUENCE=CCCACTTACCATTAGCAGGAAG
PRIMER_RIGHT_SEQUENCE=ATGGACATATGGTGCATAGCAG
PRIMER_LEFT=117,22
PRIMER_RIGHT=236,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.873
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=0.2701
PRIMER_LEFT_1_PENALTY=0.143555
PRIMER_RIGHT_1_PENALTY=0.126537
PRIMER_LEFT_1_SEQUENCE=GATCCCCACTTACCATTAGCAG
PRIMER_RIGHT_1_SEQUENCE=ATGGACATATGGTGCATAGCAG
PRIMER_LEFT_1=113,22
PRIMER_RIGHT_1=236,22
PRIMER_LEFT_1_TM=59.856
PRIMER_RIGHT_1_TM=59.873
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.2701
PRIMER_LEFT_2_PENALTY=0.143555
PRIMER_RIGHT_2_PENALTY=0.126537
PRIMER_LEFT_2_SEQUENCE=AGATCCCCACTTACCATTAGCA
PRIMER_RIGHT_2_SEQUENCE=ATGGACATATGGTGCATAGCAG
PRIMER_LEFT_2=112,22
PRIMER_RIGHT_2=236,22
PRIMER_LEFT_2_TM=59.856
PRIMER_RIGHT_2_TM=59.873
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.3092
PRIMER_LEFT_3_PENALTY=0.182645
PRIMER_RIGHT_3_PENALTY=0.126537
PRIMER_LEFT_3_SEQUENCE=AAATTCCTGAAGATCCCCACTT
PRIMER_RIGHT_3_SEQUENCE=ATGGACATATGGTGCATAGCAG
PRIMER_LEFT_3=102,22
PRIMER_RIGHT_3=236,22
PRIMER_LEFT_3_TM=60.183
PRIMER_RIGHT_3_TM=59.873
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=0.4305
PRIMER_LEFT_4_PENALTY=0.014720
PRIMER_RIGHT_4_PENALTY=0.415781
PRIMER_LEFT_4_SEQUENCE=CCCACTTACCATTAGCAGGAAG
PRIMER_RIGHT_4_SEQUENCE=TGGACATATGGTGCATAGCAGT
PRIMER_LEFT_4=117,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=60.015
PRIMER_RIGHT_4_TM=60.416
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_00800 Ps_contig_mira-and-tgicl-ass_franssen_7585
SEQUENCE=GGATSAATAGTCAAATSACAAATCTCTCTCTCTTTCTCTCTCAATCGTTGAACTTTCCAGTTTCCACTTTCCCATTTCCACATTCTTCTAACCTTCACTCACAAAACCACAATCCGATCTAGGGTTCATCACTCTCACTTCCGATGGACAAATTGAAGCTAGCTCTCTTCGGCGAGAGGCTWCAAAAACCAACAGCGCTAATCTGAGTCGAATCGTAAGCGGACAAAATGAAGAATCNCCAAACTCG
TARGET=23,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00801 Ps_contig_mira-and-tgicl-ass_franssen_7596
SEQUENCE=AAAATAAAAACCTTTTAAACAAACACTTCACCTGCAACCTCAAAACTCAACAGCCCTTTTCTTTTCTGAAACTTTTATCACAGTTACAGTTGCAATTACACGATTTTCAGATTGAGATTGGTAAATGGAAGTCATTCATTCATTCACAATCATCAAATCATCAAATAAGATAAGCATAAGATGTAAGRAATATATAGAAAATAGATACTAATGGAAAACCCAAAAACTAAAAACAAAAGAGCTTTTTTTTATAAGTTTTGTTTATTATAGAGTAACATATTATAGACACATGTACATAAAGTGCAGATCTATGTTAATCCACTTGAATTTCCCACAAAGACACAATTTTTTTAACAAATTGAAAGAACAAAAGAAACCCAAATGGTAGATAGAGAGTGAGTGTATTTTTGGATCAAAGGTTGAGGATGAGAATGGAATTGAAGAGAAGCTAGAAATGAGGATGAAAGAGAGGCTGAAATGGGTTCAGAAACGAGAAGAGTGGAATGTGAAGAGAGAGAAATGGATAGGGAAAGAGTGGGATGCGGAGAAGATGAAAATGGAGAAACAATCGGAGAAAAGCACGGAGATCGCAGTGAACCAGAGACTCGTTCTTGTGTTGAGTTTGTAAAATCTCAATTTTAGAGAGAGAAGGTCCCGA
TARGET=132,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00802 Ps_contig_mira-and-tgicl-ass_franssen_7604
SEQUENCE=CGAAACCCAACTTAGTTGGATCTTTCAAATTCGGGAAATTCAAACGCGCGTATTCTCCACGAAGCTTATAAGCTGCACGATCATAAGCATAAGCAGCAGCTTCAGCAGTTTCGTAAGTTCCAAGCCAAACTCTCATTCTATTCTGAGGAAGTCGAATCTCAGCCAACCCTACTTTTCCCCAGTGTCTCTGTCTCACTCCTCTGTATAGCTTCTTCTTCAAAGGATTGTTCACCAAAAGAAGTGAACAAACTCGAACTTGCACTTGAAATTGAATTTGTTGTTGTTGTGTATAAGGTGTGTTAGAAATGAAGATGGAATGAAAGGAGGGGGTTAA
TARGET=275,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6071
PRIMER_LEFT_PENALTY=0.026872
PRIMER_RIGHT_PENALTY=0.580189
PRIMER_LEFT_SEQUENCE=CGATCATAAGCATAAGCAGCAG
PRIMER_RIGHT_SEQUENCE=CCTCCTTTCATTCCATCTTCAT
PRIMER_LEFT=77,22
PRIMER_RIGHT=326,22
PRIMER_LEFT_TM=60.027
PRIMER_RIGHT_TM=59.420
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=0.6260
PRIMER_LEFT_1_PENALTY=0.045851
PRIMER_RIGHT_1_PENALTY=0.580189
PRIMER_LEFT_1_SEQUENCE=AGCAGTTTCGTAAGTTCCAAGC
PRIMER_RIGHT_1_SEQUENCE=CCTCCTTTCATTCCATCTTCAT
PRIMER_LEFT_1=103,22
PRIMER_RIGHT_1=326,22
PRIMER_LEFT_1_TM=59.954
PRIMER_RIGHT_1_TM=59.420
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.6605
PRIMER_LEFT_2_PENALTY=0.080323
PRIMER_RIGHT_2_PENALTY=0.580189
PRIMER_LEFT_2_SEQUENCE=TTCAGCAGTTTCGTAAGTTCCA
PRIMER_RIGHT_2_SEQUENCE=CCTCCTTTCATTCCATCTTCAT
PRIMER_LEFT_2=100,22
PRIMER_RIGHT_2=326,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=59.420
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=0.6605
PRIMER_LEFT_3_PENALTY=0.080323
PRIMER_RIGHT_3_PENALTY=0.580189
PRIMER_LEFT_3_SEQUENCE=TCAGCAGTTTCGTAAGTTCCAA
PRIMER_RIGHT_3_SEQUENCE=CCTCCTTTCATTCCATCTTCAT
PRIMER_LEFT_3=101,22
PRIMER_RIGHT_3=326,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=59.420
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.6782
PRIMER_LEFT_4_PENALTY=0.098039
PRIMER_RIGHT_4_PENALTY=0.580189
PRIMER_LEFT_4_SEQUENCE=ACGATCATAAGCATAAGCAGCA
PRIMER_RIGHT_4_SEQUENCE=CCTCCTTTCATTCCATCTTCAT
PRIMER_LEFT_4=76,22
PRIMER_RIGHT_4=326,22
PRIMER_LEFT_4_TM=59.902
PRIMER_RIGHT_4_TM=59.420
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_00803 Ps_contig_mira-and-tgicl-ass_franssen_7611
SEQUENCE=CGGGGGGTAAGGAGGGATTTGTAAGCAGTTCCACGGTTGGGAGGTGGGCGTTGCTCCCCCCAGGAGCACTAATTAGTTTTCCACAGAGATTAAAGTACTGGGGTGGTTTTCCCCATCTGAATTGCCAGAGCTGCTTCAGCATGAGTACTAAATCTCACAAACCCAAATCCTTTATCACGCTGGACACGAACCTCCTCTATCACACCAGCCCCAAGGGTATAGAAATGACGATGGAGATCAAGCTGTGTTGCCTCAGAACCAAGGTTACCCACATAAACAGTTGTATATTGAGGATTGTTCTCAGGAGCATCACTGTTAGGTGTCTCTTTACCATCCTCAGATGAGCCATTTGTTAGTTCCACCACACTTTTGTTTTCTGGGGTTTGCTTCTCTTCAATGCCACCAGCACCTTTTGTTGCCCAATTGCAGCGTATTTGTCTACTTCCAAGCCACTTACCAGTCAAATCATTTATAGCACTTTGAGCATCCTGCTGACTACGGAATGAAACAAATCCAAATCCCCTTGAGCGCCCAGTCTTCTGATCCCACATAACCCTTGCATCTGAACAACTTTGGTAGACAGAAAAGCATGCAAAAAGTGTAGCATCAGTAACCTCAGGACTGAGGTCACCAACAAATATGTTGTAATGGCCTGATGTATCCTCTCTCTGACCACTAGCATATGCCCAGTTAACTTTGATAGGTTGCCCAAAAAGATGCCTTCCGTTGAGAGTTAATATGGCCAGAGCAGCAGATCTGCGATCAAAGTAATGAATGAATCCATAGGATGACTTTTCTTTCCTAAAAAGTTTACACCCTTCAACTGGGCCAGTACCTGCAAAAACTTCTTGCAGAAGTGGCTCGGTTACCTGGGTATGAACGTTGCCCACATACACACTGCGGCAAGTACTTGGATCAAAACCAGGAGGCAGATTTCCACTTGGGATTGGTTCAATCTGAGGTGGAGCTAGGAGACCAGGGTGGTAGAGAGACTGTTGTTGAAGAAGAGCTTGTTGCATCATAGCTTGTTGTTGTTGCTGATGCTGCTGATGCTGCTGCTTCAACCTATGATTCTGCATTTCGAGGTACTTCACCGTTTTCCGGGGGGAAATCTCGCCGGAAAATGGGTTATAGGGCGGAGATTAGGGTTGAAAGGGTCCGTAGAGACACAGGCAGGCCTCTGAGAAAACAGCAGAGGAGTCCACAGAAAACCCCTCGCCGACTATTCATC
TARGET=1026,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1594
PRIMER_LEFT_PENALTY=0.124370
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=TAGGAGACCAGGGTGGTAGAGA
PRIMER_RIGHT_SEQUENCE=AAAACGGTGAAGTACCTCGAAA
PRIMER_LEFT=968,22
PRIMER_RIGHT=1099,22
PRIMER_LEFT_TM=60.124
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.2017
PRIMER_LEFT_1_PENALTY=0.124370
PRIMER_RIGHT_1_PENALTY=0.077340
PRIMER_LEFT_1_SEQUENCE=TAGGAGACCAGGGTGGTAGAGA
PRIMER_RIGHT_1_SEQUENCE=GTGGACTCCTCTGCTGTTTTCT
PRIMER_LEFT_1=968,22
PRIMER_RIGHT_1=1204,22
PRIMER_LEFT_1_TM=60.124
PRIMER_RIGHT_1_TM=59.923
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=0.2017
PRIMER_LEFT_2_PENALTY=0.124370
PRIMER_RIGHT_2_PENALTY=0.077340
PRIMER_LEFT_2_SEQUENCE=TAGGAGACCAGGGTGGTAGAGA
PRIMER_RIGHT_2_SEQUENCE=GTTTTCTGTGGACTCCTCTGCT
PRIMER_LEFT_2=968,22
PRIMER_RIGHT_2=1211,22
PRIMER_LEFT_2_TM=60.124
PRIMER_RIGHT_2_TM=59.923
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.2048
PRIMER_LEFT_3_PENALTY=0.169745
PRIMER_RIGHT_3_PENALTY=0.035021
PRIMER_LEFT_3_SEQUENCE=GACCAGGGTGGTAGAGAGACTG
PRIMER_RIGHT_3_SEQUENCE=AAAACGGTGAAGTACCTCGAAA
PRIMER_LEFT_3=973,22
PRIMER_RIGHT_3=1099,22
PRIMER_LEFT_3_TM=60.170
PRIMER_RIGHT_3_TM=60.035
PRIMER_LEFT_3_GC_PERCENT=59.091
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=0.2097
PRIMER_LEFT_4_PENALTY=0.174657
PRIMER_RIGHT_4_PENALTY=0.035021
PRIMER_LEFT_4_SEQUENCE=GCCAGTACCTGCAAAAACTTCT
PRIMER_RIGHT_4_SEQUENCE=AAAACGGTGAAGTACCTCGAAA
PRIMER_LEFT_4=827,22
PRIMER_RIGHT_4=1099,22
PRIMER_LEFT_4_TM=59.825
PRIMER_RIGHT_4_TM=60.035
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_00804 Ps_contig_mira-and-tgicl-ass_franssen_7612
SEQUENCE=AAAATGGTAGTTTGTTCTGTAGCTTTGGGTATATCCCTTTAGGGTTTAGTGGAAATTTTAAGGATATTAGTGGGTCATCTTCGGAATGGAACCCTATTTCTACTTTGTTTCCTTGCTCTATGAAAGGCGCTTCTTCCAAGAAGGGTTTTCAAAGAGAGCCTGTTGTTTATTACGATCATGATACGTGTGCGGCTTTCGCGGTGCTTTCTGATGAAACTGAAGATGATACTGATGATGATGATGATTGGTGGAGAGGGGATGATAGCAATGAGAGGAAGAAGGTGGTTATGACGAGGAGGAGTATAGAAAAGAAGAAGGACTGTTGGGGGTGGTGATGGTGATGGTGCGTGGGAAAGGGGCCTCTTGATACTGGGAGGCCCGAAGGAGGGGGGGTTCGAGAATGGTAATGCTGGCGGG
TARGET=230,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0451
PRIMER_LEFT_PENALTY=0.042816
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=ATCTTCGGAATGGAACCCTATT
PRIMER_RIGHT_SEQUENCE=CCTCGTCATAACCACCTTCTTC
PRIMER_LEFT=76,22
PRIMER_RIGHT=295,22
PRIMER_LEFT_TM=60.043
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.0451
PRIMER_LEFT_1_PENALTY=0.042816
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=ATCTTCGGAATGGAACCCTATT
PRIMER_RIGHT_1_SEQUENCE=TCCTCGTCATAACCACCTTCTT
PRIMER_LEFT_1=76,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=60.043
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.0451
PRIMER_LEFT_2_PENALTY=0.042816
PRIMER_RIGHT_2_PENALTY=0.002310
PRIMER_LEFT_2_SEQUENCE=ATCTTCGGAATGGAACCCTATT
PRIMER_RIGHT_2_SEQUENCE=CGTCATAACCACCTTCTTCCTC
PRIMER_LEFT_2=76,22
PRIMER_RIGHT_2=292,22
PRIMER_LEFT_2_TM=60.043
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=217
PRIMER_PAIR_PENALTY_3=0.0451
PRIMER_LEFT_3_PENALTY=0.042816
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=ATCTTCGGAATGGAACCCTATT
PRIMER_RIGHT_3_SEQUENCE=TCGTCATAACCACCTTCTTCCT
PRIMER_LEFT_3=76,22
PRIMER_RIGHT_3=293,22
PRIMER_LEFT_3_TM=60.043
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.0451
PRIMER_LEFT_4_PENALTY=0.042816
PRIMER_RIGHT_4_PENALTY=0.002310
PRIMER_LEFT_4_SEQUENCE=ATCTTCGGAATGGAACCCTATT
PRIMER_RIGHT_4_SEQUENCE=CTCGTCATAACCACCTTCTTCC
PRIMER_LEFT_4=76,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=60.043
PRIMER_RIGHT_4_TM=59.998
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_00805 Ps_contig_mira-and-tgicl-ass_franssen_7616
SEQUENCE=GTCRRRSAAATTAATTAGACGGAAAACGAACATACTAACGACAGCCGAACTCTCTTTCGCCCTCCGCCTCTCTCTCCGCCGCTCCCGCGCCGTCGCTCTCTCTCACACACACACTTGCACCGCCGTTCCTACTCAGAGTCACAGTTTTTTCTTGTGCTCAGTTGAGTGAGTTCAGAAGGAAGAGTGGTTTTATTTTTATCAATTTTTATGGAGATCGACAGACCTGACGGAAGGACACCCAATCAA
TARGET=104,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00806 Ps_contig_mira-and-tgicl-ass_franssen_7620
SEQUENCE=CTCTCTCTTTCTTCTCNCAAAAAANAAGTTTCTTTTTTCTTTTCTAATAATCTAAAGAAGTAAAAGACAAGTACCAAAATTAGTTGCTTGTTTGWAAAACCCTTACGAAGGAAAGTAACAAAACAAATCAAACCCCACTATTGAAAGTTTTACATTTTTAAAACTTTTAAATATAGTATAGTAGTGATGAAGTGGAAGTAAGAGAGAGAGAGAGAGCGAGAGAGGGGGAGAGAGAGAGATTCATATGAAGAGTAACGAGTATTGTGGAAGAAAGAGTCAACCTGGGAAAGCCGGGAGAGTTTTTGTTGGAAGGTATATTGGATTGCATCCATGAAATCACAGTTGCACTTTCCTTTCCAATAGAATGAGTGATTCTCTTTAACAAAGTTGATAACAACA
TARGET=201,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00807 Ps_contig_mira-and-tgicl-ass_franssen_7620
SEQUENCE=CTCTCTCTTTCTTCTCNCAAAAAANAAGTTTCTTTTTTCTTTTCTAATAATCTAAAGAAGTAAAAGACAAGTACCAAAATTAGTTGCTTGTTTGWAAAACCCTTACGAAGGAAAGTAACAAAACAAATCAAACCCCACTATTGAAAGTTTTACATTTTTAAAACTTTTAAATATAGTATAGTAGTGATGAAGTGGAAGTAAGAGAGAGAGAGAGAGCGAGAGAGGGGGAGAGAGAGAGATTCATATGAAGAGTAACGAGTATTGTGGAAGAAAGAGTCAACCTGGGAAAGCCGGGAGAGTTTTTGTTGGAAGGTATATTGGATTGCATCCATGAAATCACAGTTGCACTTTCCTTTCCAATAGAATGAGTGATTCTCTTTAACAAAGTTGATAACAACA
TARGET=228,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00808 Ps_contig_mira-and-tgicl-ass_franssen_7641
SEQUENCE=AAACAGAAGATTTCAAATAACTTATTTCAAATGCAAAGATAACCATTGCAGATTTTTCCTCACAATTACAAAAAGAACAACAACTGCAGAATATTCAGAAACTTATTAACATCAAAATGAATCTCAAATACATTTAATACTTTTTCATTTCAATTTGTATTTCACTTCACCTCAGTCTAGTAGCTGAAAAATCACCTAACAAGCTAATAGAGTATTTAACTAGCTAACATGGTCGAAGCATAATAACCTGCAGAATGAAATTCAAGCAAAAAAAGYATTTGGAAACTAACTCAATTTAAAAGATAATTTCATATGCATTTCTAATTGGAATTCCTAATTTCTAACTAACTAATGCATTTCCATTTCATAGAAGCTAGTGGCAATCTGGATTTGGATTTTGGAGGGGATTGGATGGTGGAGCCTTGTTTCTTCATCCCGTCTTCGCTCCGATCTCAAATTCGCCGCGAAAAGCGCAACACGAACGGACCTTTCAAAATTGGCGCAACATCGTCTTGAT
TARGET=341,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00809 Ps_contig_mira-and-tgicl-ass_franssen_7658
SEQUENCE=AGTCCAAAGATCGTGGAGATGCAGATGCTACCGYTGAGGTGTTGCTCTGCCCTTCCTCTTCAGGAACCAGCAGCGGTGGCGGAGGCATCAGATTCCAAATATACAAACTATGGAAGTGGAAGAGTTGGTGTAGCAGAAGAAGAAGAGAGATTATTCTTTGTTGTTTGAAGAGTTGGCAGAGAAGAGTGAAACCTAGAGAGAGAGAGAGAGAGAACTCAAGGGTCTTTCATTCCAATAAGCCACCCCGAC
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00810 Ps_contig_mira-and-tgicl-ass_franssen_7658
SEQUENCE=AGTCCAAAGATCGTGGAGATGCAGATGCTACCGYTGAGGTGTTGCTCTGCCCTTCCTCTTCAGGAACCAGCAGCGGTGGCGGAGGCATCAGATTCCAAATATACAAACTATGGAAGTGGAAGAGTTGGTGTAGCAGAAGAAGAAGAGAGATTATTCTTTGTTGTTTGAAGAGTTGGCAGAGAAGAGTGAAACCTAGAGAGAGAGAGAGAGAGAACTCAAGGGTCTTTCATTCCAATAAGCCACCCCGAC
TARGET=195,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00811 Ps_contig_mira-and-tgicl-ass_franssen_7659
SEQUENCE=GGTGCTTATTGGAATGAAAGACCCTTGAGTTCTCTCTCTCTCTCTCTCTCTTAGGTTCACTCTTCTCTCGMCAACTTCTTACAAAACAAACAAAAGAATAATCTCTCTTCTTCTCTGCTAACCAACCAACTTCCTTCCACCTT
TARGET=31,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00812 Ps_contig_mira-and-tgicl-ass_franssen_7661
SEQUENCE=TGCAACCCACTATAACGGCGTCTTGGAGTATGATGCTCATAGTATCTATGGTTTCTCGCAATCCGTCGCAACACACAAGGGTCTTCTTGGAATTGAAGGTAAAAAGGCCTTTTATTTTGTCGCGATCCACTTATGTTGGTTCGGGGAAATATGCAGCACATTGGACAGGTGACAATCAAGGAACATGGGAGAATTTGAGGTATTCAATATCCACAATGCTGAACTTCGGTATATTCGGTGTTCCAATGGTTGGTTCAGACATTTGTGGATTCTATCCGCAACCAACTGAAGAACTATGCAATCGATGGATCGAAGTAGGGGCTTTCTACCCTTTTTCAAGAGATCATGCAAACTACTACTCCCCTAGACAAGAGCTTTACCAATGGGATTCAGTAGCTCAGTCAGCTAGAAACGCTTTAGGTATAAGATACAAGATTCTCCCATATCTTTACACCTTAAACTATGAAGCTCATGTCAGTGGATCACCAATTGCAAGACCACTTTTCTTCACTTTCCCATCTTACACCGAATGTTACGGCCTCAGCACTCAGTTTTTGCTCGGAAGCAGTCTCATGATATCGCCGGTGCTCGAGCAAGGAAAAACACAAGTGAAGGCATTGTTTCCTCCCGGTAGTTGGTACAGTTTACTTGATTGGACTCACACCATAACATCAAAAGGCGGAACTTATGTTACACTCGACGCGCCTCTTCATGTAGTAAATGTCCATTTATATCAGAACACAATTCTTCCAATGCAGCAAGGTGGTTTGATCTCCAAGGAAGCTAGAACAACACCGTTCACACTCATTGTAACATTCCCGGCTGGCGCAACAGAAGGAGAAGCTAAGGGTACACTTTTCATCGACGACGACGAACGTCCAGAAATAAAGCTTGGAAATGGATATTCGAGTTTTATCGATCTATACGCGAGTGTGAAACAAGGAGGTGTGAAAGTGTGGTCAGAAGTTCAAGAGGGTAAGTTTGCTTTAGACAAAGGTTTGATTATTGATTCAATATCTGTGTTGGGATTGGATGGAAATGTTGGAGCAGTGGCTTCACTTGAGTTAGATGGAAAACCATTGATTGGTAAGTCAGGTTTGGATGTTACTACTTCAGAACATGTAGATTTGAATGGTGAAGGAAATGGAGAAAGTAAGACTGTGATGGTTGCATTGAGAGGTTTGAGCATCCCTGTTGGTAAGAACTTTGCTATGACATGGAAAATGGGATGATGATTTAGATTGACTGAGTATTTGTGTTATGTTAGCTTTGTTTTATTTTTCTAGTTTTAAGTAATGAACRAAAGAAAAAAGA
TARGET=863,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00813 Ps_contig_mira-and-tgicl-ass_franssen_7664
SEQUENCE=ACGGTCATTTATTANCNCATATACATATACTATANGANNATGTTGTTTATATAGTAACATTACAAAGAGCTTTTCATTGTTATTTTCACCACTATAAAATACAAATCGTTTAATTTTTTGGGTAAGTAGAAGCAAACACAACCACAATCTCAATCTCAAAACTCATATGCAAAACGCCAAAAAAAACCTTAAACCTTAAGAAATTCCATGATGTATGTGTTGAAAGCATTACAATGGACTTTGAAACCTTGGAATCCAGCACCTTTAGCAAGATCCTCAAACTCTTTCTGTGTTCTCTCTTTCCCACCTGGATTATGAGCCAACATGATCACATCAATGTGCACCACACCTTTTGTTGCCAAGCTTGTGTCTGGTGCCACTGGAAGAATGCATTCTGCCACAATCACTTTTCCATTTTCAGGAAGTGCCTCATAACAGTTCTTCAAAAATTTCAAGCAGTGCTCATCACTCCAATCATGACAAATCCACTTCATAAAAATAGCATCAGCCTTTGGAACACTGAGAAACATGTCTCCGCCAACATGCTCCACTCCGGGATAAGAAGGAGCGTCTTCAATGACATGAGGCAAATCAAAATTAATCCCTTTAATGGTGGGATATTTTGAGACAATGGTGTTAATTACAGCTCCGGTGCCACCACCTACATCAACAAGAGATTTAAGGCCTTGAAAACCTGTGTAGGTCTCAAGAATTTTCTTCATTGTGATGGTAGAGTGATCAGACATTCCCTTGTTGAAAACTTTGTTAAACCTTGGATCTGTTCCATGGTATTCAAAGGCTGTCATTCCATAAGCTTTGTTGAATGGAATGCCCCCATCAAGGACAGCATCTTTAAGGTGGTACCAGCTTTCCATGAGTACTTTATCCTGATTCATGAGATTAAGAGCAGAAATAGAAACACCATCTTCATTCTTAACCAAGTACTTAGCAACAGTAGCTAAACCATAGAGTCTCTGAACCTTTCCATCTTCTAGAGTCCTTAACGTACAAGTAAGAATATTGTAACAAGCCAATAGCCGCAAGATACGGTCCAACATAACAGGTGCATCAGGGTTCTTTGTTGGAAGCTGAGAAGCAATATCTAGCGGCGAAAGATGAGCACCAGGACCAGATTTAGCTATGATTTCTAAGAGATCAAGTTCAAGAGCTGTTTTTAAGACCATTGGAAGAACAGAAGCACTTGCTAGCTGCATAGCGAAGAGATTTGCTTCTTCGTCTGATACGTGGGTTGGTGTTATTTGTGTCTCACTTGTTGTTGTTGAACCCATTGTTGTTTTGGTTAAGGTTTGAGGAGATTGAGGTGGTGATTGGAAGAAGAATGGTCCATGAATTTCAATGTGGCTTTGTGTTATATAGAACAACAACGTATGGGAAAAATGTTTGGTTGGGTTTGGTGTGTTGGTTCAATCTATTTAGATATGGGTCCCACTCAACAACCACGTCTAGGACCC
TARGET=1271,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2048
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=0.186949
PRIMER_LEFT_SEQUENCE=TGTTGGAAGCTGAGAAGCAATA
PRIMER_RIGHT_SEQUENCE=CATGGACCATTCTTCTTCCAAT
PRIMER_LEFT=1079,22
PRIMER_RIGHT=1348,22
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=60.187
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=270
PRIMER_PAIR_PENALTY_1=0.2071
PRIMER_LEFT_1_PENALTY=0.017846
PRIMER_RIGHT_1_PENALTY=0.189235
PRIMER_LEFT_1_SEQUENCE=TGTTGGAAGCTGAGAAGCAATA
PRIMER_RIGHT_1_SEQUENCE=GGACCATTCTTCTTCCAATCAC
PRIMER_LEFT_1=1079,22
PRIMER_RIGHT_1=1345,22
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=59.811
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.2071
PRIMER_LEFT_2_PENALTY=0.017846
PRIMER_RIGHT_2_PENALTY=0.189235
PRIMER_LEFT_2_SEQUENCE=TGTTGGAAGCTGAGAAGCAATA
PRIMER_RIGHT_2_SEQUENCE=GACCATTCTTCTTCCAATCACC
PRIMER_LEFT_2=1079,22
PRIMER_RIGHT_2=1344,22
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=59.811
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.2952
PRIMER_LEFT_3_PENALTY=0.108206
PRIMER_RIGHT_3_PENALTY=0.186949
PRIMER_LEFT_3_SEQUENCE=ATAACAGGTGCATCAGGGTTCT
PRIMER_RIGHT_3_SEQUENCE=CATGGACCATTCTTCTTCCAAT
PRIMER_LEFT_3=1056,22
PRIMER_RIGHT_3=1348,22
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=60.187
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.2974
PRIMER_LEFT_4_PENALTY=0.108206
PRIMER_RIGHT_4_PENALTY=0.189235
PRIMER_LEFT_4_SEQUENCE=ATAACAGGTGCATCAGGGTTCT
PRIMER_RIGHT_4_SEQUENCE=GGACCATTCTTCTTCCAATCAC
PRIMER_LEFT_4=1056,22
PRIMER_RIGHT_4=1345,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=59.811
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_00814 Ps_contig_mira-and-tgicl-ass_franssen_7665
SEQUENCE=AAGTTAATTAGTGGTTTTTTTCGTTTACGTAGGTAAATTAGGTAACAGAAAGAGGGAAGGGACGTTTGTAAACTTACTTTCGTATCTTTGTAGGAAATAGTTCATTCTACNGKTTTGATGAATTGATTCAGAGAGCTTCTGATTGTGTTTCGTCTCCAAGTGGTTCTACTGCCGCTTCGAATTCGAGGAAAATTGCTTACTCTAATGGTTCTGCTACCGCGGTTTCGACAAGTTTATCGAAGAATTTGACTTCTGGTTCTGGTTCTCGAGGTGGGTTTGAGAAGCAGGTGCCTTCGATGGCTGCTTCTTCCACTTTGGTTCATGTGGAGAGAGAAGTTTCTGCAAAGGCTTCCGAATTGTTCACAAGAGATGATAGTTATGTGAGCAAGTCCAAATCTACATTCTATCCCAACGGGTATGGGATTGAACCAAATTTTGCATCCAAACCCCAAATAATGGATTCATCGTTAAAATCCACAACTGCTGCAGGTCAAGATGGTGACAAGTTGAGTTTGATCAAACTTGCTGGCATAATTGAGGTTTCTGCGAAGAAAGGAACTCGAGATCTCAAGCTCCAGGGAAAGCTGATGGACCAAGTTGATTGGCTACCTGATTCGATAGGAAAGTTATCTAGTTTGGTTACCCTTGATTTATCAGAGAATAGGATTGTGGCTATACCTTCAACAATTGGAGGCCTTTCATCATTGACCAAATTGGACTTGCATTCCAATAGGATCACAGAGATTCCTGATTCTGTTGGAAATCTCTTAAGTCTGGTATATCTAAATCTGAGGGGAAACCAGTTAACAACCTTACCTGCTTCTTTGAGCAGATTGATATGTCTCGAGGAGCTTGATTTGAGTTCAAATCTAATATCAGTGCTTCCTGACACTATAGGGTCGCTTGTTAACCTTAAAGTGTTGAATGTGGAAACTAATGATATAGAAGAAATTCCACATTCTATTGGTAATTGTTCTTCCCTTAGAGAGCTTCAAGCGGACTATAATCGGCTTAAAGCCCTACCAGAAGCTGTAGGGAAGATTGAGAGTTTAGAGATCTTGTCTGTGCGGTACAACAACGTCAAACAACTACCAACAACAATGTCGTCTATGATAAACCTGAAGGAACTTAATGTGAGTTTCAACGAACTTGAGTCCGTTCCTGAGAGCTTATGTTTCGCGACCTCCCTTGTCAAGATGAACATAGGTAACAACTTTGCTGACATGAGATACTTACCAAGATCAATTGGGAACCTCGAAATGCTCGAGGAATTGGATATCAGCAATAATCAGATACGGGTTCTTCCCGACTCATTTAGGATGCTTACAAAACTACGTATCTTGCGAGTTGAAGAAAATCCTCTTGAGGTTCCTCCAAGAGAAATAGCTGAGAAAGGAGCACAGGCTGTTGTTCAGTACATGGCTGATTTTGTGGAAAAGAGGGAAAAGAAAGATGTTAAACCAGTTCCAATTAAGCAGAAAAAGACCTGGGCTCAGATCTGCTTCTTTTCAAGGTCTAACAAAAGAAAGCGCGATGGAGTTGATTATGTGAAAGCTTGATTCCATTAGTTTCACTACATGTGGCATGGCTATATTTCATCCTAGGAAAGAAGGAAAGGAATGGCTGCTGATATTCTCTCTATTTTCCTGTTTGGAATGATCTATCTTTGCATTGAGAATAGAGACAAAACAAAATATCCTAATGGTGGGTGGGAGAATGAAGTGACAGCATTGGTTGGCTGTTACTAGAATTCCCTGTTGAGATTGCAGCAAAAATTTGTAGATTTGTTGTTCAGTATTTTCTTTTTGAATTTTCCATTTATTTCATTTTGTATTTGTATTATCTTGAAATTAGAATGTGTACACAATCTAATAGGAAAGCAGAGATGATGGAGATAAATTGAATGTGATTATGGACCAGTGTTTATGTATCATGTATGTATCTATGTATGTATGTACAATTGTACTTTTAATCCATTGTTAAATGATTCGTAGTTACTRAAAGTT
TARGET=1944,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00815 Ps_contig_mira-and-tgicl-ass_franssen_7685
SEQUENCE=TTTMYTTCTTATGCTATGTAATTCTACCTAAATGGAKTTACATATTTTTATGTTGTGCAGGAACTACTACAGGTGCACTCACAAAATTGAACRAGGTTGCAAAGCAACAAAACAAGTGCAAAAAATTCAAGAGGATCCACCTCTTCACAAAACAACTTACTATGGTCATCACACTTGTAGAATCTTACAAGCCCTGAGATCATTGTGGATTCATCACTTTCTCCTTCTCATCACTCTGCGTTGATACCACTTTGACAACTCTTTCCCAACACCAGCCAAACAAGACTGCCGTTTTCTCTCATCACCTTCTTATCCATCAACATCATTATCAATAATATCATCATTGAAAAGGGAGTGCAAGGAAGAGATTGTTCATCCTCCTCCTTCATCTTCAGCTAATGATTATTACCTCTCTGGACTCACTTTTGATGATTCAGAAAAGGATGTCACACTATCATCAACCCTTGATTCTCATCAACTAGGTGTCAATATTCCTGATATCTTGTATGATGATGTCCTTAATTGGCCTCTTTCTTGAAAKTTGATCAAGTAGTCAACTATGTTTTGGTCAATGTAATTTTCCTTTGTCTTAGTGTGTTTTTCCATTATGGTATTTTAGCTTAATATGGTTGATAGCAAGTAGGAGAGTATTTAGTTTAGTATGGTTGACTAGCTAGCTAGTCTTGGCTTTCTATTTTGAATAGTAAAAATTGTTGGGTTTTTTAGTTAAATTTTAACTTACCCCTTAATTTTTAAA
TARGET=670,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00816 Ps_contig_mira-and-tgicl-ass_franssen_7722
SEQUENCE=GAAATAAATAAATACCAAATAATAAACAGAGTAAGAAACSACAAGGATTCAGAATCAACCAACAAAGAAGGGGAATCACATTTCTGCAAAAAATGGAATCACCGAGCA
TARGET=2,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00817 Ps_contig_mira-and-tgicl-ass_franssen_7731
SEQUENCE=TTAGGACCATGCTCGAGCCTATGAGTATCCAATTCTTTGTCCATAGTTTCTTGTAAATTCTGGTCATTACTGTTTGCTTTCCTTGCATTCCTCGCATTCTGCCATCTCTCCAAATCCTTCTCAACATTGGCAAGATACTGGTCCCCAATTTGTTTTTGATACTCAGTCAATTCCTCTCTTCTTGCATTCTTCCATTCAAGGAAATCCTCTTCTATGCGTTTCTCAGCTGATGCAGCATCTTCATCCAAGGGCATGTTAGGCATATAGTAGATTGGAGGCTCTGCTTTAGTCCTGCAAAAATACATTCAATCAAATAACAGACGAAAATTATCAAAGAAAACTTTAATTATTTAAAATCCCGCACTTAGCAGGAACATCGAGTCATCAAATTGCATCAATATTACAATGCTCGTGAACAGAAACTTATCTTCGTCTCAATAAAATGTGAATATGGACACTTTTGGACCAAAATTAAAAATAACCTTATAAAATTGCTTAGTCGTTTATGATGCTCACTCCACCGAAGAAAGAGTAACTCCAATTTCTTTTCTTCTGTCTTAGCAGCCACACGTGCCCTAAGTGTCAAATCCCTCCTCCGTTTTTCAGCAATCTGCTCACGCTCTTCTTTCCTAAGCCTTTCACTTTCTTCCCGTGCTCTTTGCTCAGCTCTTTGCAACGAATTAGATCTTCGCATATATGCCTCAGTTCCTGAGAGCTGCTTGTCTTCTTTTCTGAATTTCTCCAGAGTTCCCAAAAGCTGACCCAGCATTCTTTTGTTCCTATTAACCAAGCTTGGGTCCTCGTTCTTGGGCAATACCCTCGGAACATGTTCTGAAGCAGGAATCTCAAAAGCCTTTGAATTTCTTTGATAGCCATCTCTTCTAGAAAATCCACTTTGGTGAGAATTAAAACGGTTCACGTCATTCTGACCAACAGTAGCATTCCCATTTTCACTTTTTCCAATTGAATCCTTTGTGTTCCCAGGATCAGCATCTTCAAGCAACTCCCCTTCCTCAAGCTTAACAATAGCAGAAGAAAGTCGACGTTTAGCAGGAGGTTGATCTTCAGAATCATTATTATCTACACCAGGACGAAGGAAGGAACGTTGACGAATAGCATTGTTGCGTAGAAGAGGAGCTGGTAAGGCACCTTTGCGAAGGCCACGAGGATCTCGAAGCCTCTCAGTTATCTCGCGCTGTTGGCGAAGAAGCTCATCGATCTCGCTGCGTAGTTCTTGTTCGGTCTTCTCCGCCGCCGCCGTACTCCCCATTGGTGTCTGTCAGCAGAAAAAGGAACTATCTTCAGAGTGTCTGCAAATTATAAAACCCTCGCAATGCATCCCCGA
TARGET=1249,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0077
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.006957
PRIMER_LEFT_SEQUENCE=AAGGAAGGAACGTTGACGAATA
PRIMER_RIGHT_SEQUENCE=GCGAGGGTTTTATAATTTGCAG
PRIMER_LEFT=1093,22
PRIMER_RIGHT=1331,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.0239
PRIMER_LEFT_1_PENALTY=0.016950
PRIMER_RIGHT_1_PENALTY=0.006957
PRIMER_LEFT_1_SEQUENCE=GGATCTCGAAGCCTCTCAGTTA
PRIMER_RIGHT_1_SEQUENCE=GCGAGGGTTTTATAATTTGCAG
PRIMER_LEFT_1=1166,22
PRIMER_RIGHT_1=1331,22
PRIMER_LEFT_1_TM=59.983
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=166
PRIMER_PAIR_PENALTY_2=0.0347
PRIMER_LEFT_2_PENALTY=0.027715
PRIMER_RIGHT_2_PENALTY=0.006957
PRIMER_LEFT_2_SEQUENCE=AAGGAACGTTGACGAATAGCAT
PRIMER_RIGHT_2_SEQUENCE=GCGAGGGTTTTATAATTTGCAG
PRIMER_LEFT_2=1097,22
PRIMER_RIGHT_2=1331,22
PRIMER_LEFT_2_TM=60.028
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=235
PRIMER_PAIR_PENALTY_3=0.0590
PRIMER_LEFT_3_PENALTY=0.052069
PRIMER_RIGHT_3_PENALTY=0.006957
PRIMER_LEFT_3_SEQUENCE=AAGAAAGTCGACGTTTAGCAGG
PRIMER_RIGHT_3_SEQUENCE=GCGAGGGTTTTATAATTTGCAG
PRIMER_LEFT_3=1032,22
PRIMER_RIGHT_3=1331,22
PRIMER_LEFT_3_TM=59.948
PRIMER_RIGHT_3_TM=59.993
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.0674
PRIMER_LEFT_4_PENALTY=0.060408
PRIMER_RIGHT_4_PENALTY=0.006957
PRIMER_LEFT_4_SEQUENCE=ACGTTGACGAATAGCATTGTTG
PRIMER_RIGHT_4_SEQUENCE=GCGAGGGTTTTATAATTTGCAG
PRIMER_LEFT_4=1102,22
PRIMER_RIGHT_4=1331,22
PRIMER_LEFT_4_TM=60.060
PRIMER_RIGHT_4_TM=59.993
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_00818 Ps_contig_mira-and-tgicl-ass_franssen_7771
SEQUENCE=TATGATTCAATCATATGTGAAGCAATGAGAGTTTTTACAAACCAAGTTGATGAATACTAAAGGAACCAAAGTAGTTGCTTTTGGGGCTATGGCTTTTGTTGCTATTAGTGTTCCATTTTCTTCTTTTCTTGTTTTTCTTCATCTTTTGGAATTTGCTAAATCTTTTGATGTGATTAATTTAGTGTTTTGAGTTAGTGGATTGGTATTTGGAAAGCCAACAAATTCATTTTAAATATATATTTTGGGATTATATATCATACATACATACTAGTAATTAG
TARGET=256,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00819 Ps_contig_mira-and-tgicl-ass_franssen_7776
SEQUENCE=CAACGACTTAAAAACAGGGAGTGAAGCTTCGACATTTCTGTATGGAGGAGTGTTCCTTTTGCTGGATGGATGGATTATTGTAAAGAAAAAACCAGCAATTTAACATGAGTGATGAATGGGGTAGAAGCTAGAATCTAGGACTCTTTTGGTTACTTGATGGAGTCTTGTACAATTATTGGAATTGGCTTTGCATATGAGAAGCGCATTGTNTATATGTTGTAGATTTCTTTATATAGTGGATGTTGAAATNTCGAAA
TARGET=63,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1400
PRIMER_LEFT_PENALTY=0.108206
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=GGAGTGAAGCTTCGACATTTCT
PRIMER_RIGHT_SEQUENCE=GCTTCTACCCCATTCATCACTC
PRIMER_LEFT=17,22
PRIMER_RIGHT=127,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=111
PRIMER_PAIR_PENALTY_1=0.4628
PRIMER_LEFT_1_PENALTY=0.431057
PRIMER_RIGHT_1_PENALTY=0.031769
PRIMER_LEFT_1_SEQUENCE=TTCGACATTTCTGTATGGAGGA
PRIMER_RIGHT_1_SEQUENCE=GCTTCTACCCCATTCATCACTC
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=127,22
PRIMER_LEFT_1_TM=59.569
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.9067
PRIMER_LEFT_2_PENALTY=0.874885
PRIMER_RIGHT_2_PENALTY=0.031769
PRIMER_LEFT_2_SEQUENCE=GAGTGAAGCTTCGACATTTCTG
PRIMER_RIGHT_2_SEQUENCE=GCTTCTACCCCATTCATCACTC
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=127,22
PRIMER_LEFT_2_TM=59.125
PRIMER_RIGHT_2_TM=59.968
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=110
PRIMER_PAIR_PENALTY_3=0.9340
PRIMER_LEFT_3_PENALTY=0.108206
PRIMER_RIGHT_3_PENALTY=0.825798
PRIMER_LEFT_3_SEQUENCE=GGAGTGAAGCTTCGACATTTCT
PRIMER_RIGHT_3_SEQUENCE=TACCCCATTCATCACTCATGTT
PRIMER_LEFT_3=17,22
PRIMER_RIGHT_3=122,22
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=59.174
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.9340
PRIMER_LEFT_4_PENALTY=0.108206
PRIMER_RIGHT_4_PENALTY=0.825798
PRIMER_LEFT_4_SEQUENCE=GGAGTGAAGCTTCGACATTTCT
PRIMER_RIGHT_4_SEQUENCE=ACCCCATTCATCACTCATGTTA
PRIMER_LEFT_4=17,22
PRIMER_RIGHT_4=121,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=59.174
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00820 Ps_contig_mira-and-tgicl-ass_franssen_7778
SEQUENCE=CGSGGGGGGGTTGTTACAAAAACTCAACAAAAATTTGGATCTGATTGGGCGGCGACGATGGAGAACAACGGCAGCCTTATATCGAGAGAGAAGATCGATCAGATGGCGACCTGGGTGGGATCCACCGTCTCATCCGCTTTCTTCTCTTCCTTGGAACGCTTTTCCTGCGTCAACGTCGCTACTTCTGACCCTGACAACGACGAAGACGACGACGACGATTACTCCGTCAGTACAACCGCCACCACCACCGTCGACAATCCTAATTCCTCCGTTCAGAACACAAACGACGTCGCCAACCTCCCTGTTTGAATCATCGATACGACATCGTCGTTTCCGGTTCGCTGATCAAAATGATAATCAATCATTTTCTGCACCTTTAAATTCTTGTGAAAGTTGA
TARGET=205,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00821 Ps_contig_mira-and-tgicl-ass_franssen_7779
SEQUENCE=ACTCGTCGTTTCCGGATCAAAATGATAATCAATCATTTTCTTGCACCTTTAAATTCTTGTGAAAGTTTATGATAGTGTACACTAACTCTAAGGCTAGGAAAGTTTGTCAAGTGGAGTGCCTTTACTTTTGGCTATGTGGGATGAGAGACATGGGTAATGAGTAGTATCTCTATAAACCAACATGTTATATATATATATATAGTTAGGTTCTATTCTTTCTACTTCTACTTACTTACCTTCGTCTTGGTGTCGTTGTTGGT
TARGET=186,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6869
PRIMER_LEFT_PENALTY=0.107351
PRIMER_RIGHT_PENALTY=0.579590
PRIMER_LEFT_SEQUENCE=CCTTTACTTTTGGCTATGTGGG
PRIMER_RIGHT_SEQUENCE=CGACACCAAGACGAAGGTAAGT
PRIMER_LEFT=118,22
PRIMER_RIGHT=251,22
PRIMER_LEFT_TM=59.893
PRIMER_RIGHT_TM=60.580
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.7864
PRIMER_LEFT_1_PENALTY=0.206859
PRIMER_RIGHT_1_PENALTY=0.579590
PRIMER_LEFT_1_SEQUENCE=GGAGTGCCTTTACTTTTGGCTA
PRIMER_RIGHT_1_SEQUENCE=CGACACCAAGACGAAGGTAAGT
PRIMER_LEFT_1=112,22
PRIMER_RIGHT_1=251,22
PRIMER_LEFT_1_TM=59.793
PRIMER_RIGHT_1_TM=60.580
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=140
PRIMER_PAIR_PENALTY_2=0.7889
PRIMER_LEFT_2_PENALTY=0.209343
PRIMER_RIGHT_2_PENALTY=0.579590
PRIMER_LEFT_2_SEQUENCE=AAGTTTGTCAAGTGGAGTGCCT
PRIMER_RIGHT_2_SEQUENCE=CGACACCAAGACGAAGGTAAGT
PRIMER_LEFT_2=99,22
PRIMER_RIGHT_2=251,22
PRIMER_LEFT_2_TM=60.209
PRIMER_RIGHT_2_TM=60.580
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=153
PRIMER_PAIR_PENALTY_3=0.7889
PRIMER_LEFT_3_PENALTY=0.209343
PRIMER_RIGHT_3_PENALTY=0.579590
PRIMER_LEFT_3_SEQUENCE=AAGTTTGTCAAGTGGAGTGCCT
PRIMER_RIGHT_3_SEQUENCE=ACGACACCAAGACGAAGGTAAG
PRIMER_LEFT_3=99,22
PRIMER_RIGHT_3=252,22
PRIMER_LEFT_3_TM=60.209
PRIMER_RIGHT_3_TM=60.580
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.7889
PRIMER_LEFT_4_PENALTY=0.209343
PRIMER_RIGHT_4_PENALTY=0.579590
PRIMER_LEFT_4_SEQUENCE=AGTTTGTCAAGTGGAGTGCCTT
PRIMER_RIGHT_4_SEQUENCE=CGACACCAAGACGAAGGTAAGT
PRIMER_LEFT_4=100,22
PRIMER_RIGHT_4=251,22
PRIMER_LEFT_4_TM=60.209
PRIMER_RIGHT_4_TM=60.580
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_00822 Ps_contig_mira-and-tgicl-ass_franssen_7780
SEQUENCE=ACCGTCTCATCCGCTTTCTTCTCTTCCTTGRACGCTTTTCCTTCGCGTCAACGTCGACTACTTCTGACCCTGACAACGACGAAGACGACGACGACGATTACTCCGGCAGTACAACCGCCACCACCACCGTCGACAATCCTAATTCCTCCGTTCAGAACACAAACGATGTCGCCAACCTCCCTGTTTGAAT
TARGET=84,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00823 Ps_contig_mira-and-tgicl-ass_franssen_7812
SEQUENCE=AGCACCCAAATCTGCTCCAGTGACTTATTCACCAGCTCCAAAGGTTTCACCACCATCAAGTCCAATAGTCTCTCCACTCCAACCACCAAACACTTCCCCTGTCTCAACTCCCACGTTGCCACCACCACCAAATATATCGCCAGCGCCAGTTCAAACAACACCTGCCCCCGCACCAGTAAAGGCAACACCAACGCCAGCACCTGCACCCACAAAGCAAGCCCCAGCTCCTACACCGATTACGTCACCACCATTACCTGCAATAAACGCCCCTGGACCAGCACCCGAGTCCCCCAAGCACAAGAAAGGAAGACACAAACACAAGCACAGGAGACATCATGCACCAGGGCCAGCACCAACAATTATTCACAAGAGTCCCCCTGCACCACCAACTGATACTAAACCAGATGACGACACAGCACCGGCACCAGCACCTAGTCTTAATTTGAATGGCACACCATCAAACCATCATCAAGGGAGAAATATATGGGCAACAGCTGGATTTGCTATCACTGTTTTTCTAGCGGTAACTGTCTACAGCAGCTAGTCTAGGTGCATTGTACTCTATGGATTCATTACTAAGCAGTTACTTTGGG
TARGET=119,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1815
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.180765
PRIMER_LEFT_SEQUENCE=TTATTCACCAGCTCCAAAGGTT
PRIMER_RIGHT_SEQUENCE=TGCTTGTGTTTGTGTCTTCCTT
PRIMER_LEFT=24,22
PRIMER_RIGHT=323,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.819
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.2428
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.242059
PRIMER_LEFT_1_SEQUENCE=TTATTCACCAGCTCCAAAGGTT
PRIMER_RIGHT_1_SEQUENCE=TTTATTGCAGGTAATGGTGGTG
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.758
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=0.2443
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.243575
PRIMER_LEFT_2_SEQUENCE=TTATTCACCAGCTCCAAAGGTT
PRIMER_RIGHT_2_SEQUENCE=TTATTGCAGGTAATGGTGGTGA
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.244
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.2484
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.247631
PRIMER_LEFT_3_SEQUENCE=TTATTCACCAGCTCCAAAGGTT
PRIMER_RIGHT_3_SEQUENCE=TATTGCAGGTAATGGTGGTGAC
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=59.752
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.3281
PRIMER_LEFT_4_PENALTY=0.147295
PRIMER_RIGHT_4_PENALTY=0.180765
PRIMER_LEFT_4_SEQUENCE=TCACCACCATCAAGTCCAATAG
PRIMER_RIGHT_4_SEQUENCE=TGCTTGTGTTTGTGTCTTCCTT
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=323,22
PRIMER_LEFT_4_TM=59.853
PRIMER_RIGHT_4_TM=59.819
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_00824 Ps_contig_mira-and-tgicl-ass_franssen_7813
SEQUENCE=GAGCACCAAAATCAAAACCAAAACTTGTCAGCATACCATTGAAATCAGGTTTATTAATTGGTTCAGGGAATTATTTTACAAAAATAGGACTTGGTAGCCCTACAAAGTACTATAGCATGCTTGTAGACACAGGTAGTTCCTTCTCATGGCTCCAATGTCAACCTTGTACTATATACTGTCATGCACAAGATGATCCTATTTTCAATCCTTCCTCGTCTAATACCTATAAAACCTTACCGTGTTCGTCCCCTCAATGTTCTTCTCTCAAATCTTCCACACTCAATGAACCAAGTTGTTCAAAAGTCTCGAATGCTTGTGTCTACAAAGCAAGCTATGGCGATTCATCTCTCTCGCTCGGTTACTTGAGTCAAGATGTTTTAACCATAACACCGTCAGAGAGACTACCGGCTTTTGTATACGGATGTGGACAGGAAAATCAAGGTTTGTTTGGAAGGTCGTCAGGTATAATCGGTATGGCCAATGACAAACTATCCATGCTTGCGCAATTGTCTGGAAAATACGGAAACGCCTTTTCCTACTGTCTTCCTACGTCTTTTTCAGCACCAAACTCTACCAAGGAAGGTTTCTTGTCAATCGGAACATCATCATTGACACCTTCGCCGTACAAATTCACTCCTATACTTAAGAATCCAAAAGTTCCTAGCTTATATTTTATAGATTTGAATGCAATCACTGTGGGAGGAAAGCCATTGGGAATCGCAGCATCGAGTTATAAGGTTCCTACTATCATCGATTCTGGCACGGTTATTACAAGGCTACCTACGCCTGTTTATACGGCCCTGAAAAATGCTTTAGTGACGATCTTGTCCAAGAAATAATAAACAAGCACCAGGAATTTCTTTATTGGATACTTGTTTTACAGGTAGTGTTAAGGAAATCTCAGAATCAGTGCCTGTGATTGCGCTTATATTTGGAGGAGGTGCTGAGTTACCACTTACCGGTCACAATACCCTCTTAGAACTTGAGAAGGGTGTTACTTGTTTGGCTATAGCAGGTAATTCTCAGACAAGCTCTATTGCCATTATTGGGAATTATCAACAGCAAACATTTAAGGTCGCTTATGATGTTGCCAATTCCAAACTTGGCTTTGCACCTGGTGGCTGCCAGTGATTCCTAACCAAATCAGTTTCAGTTCACAAAGCTCTCTGTGACAATTTATCAAATATGTTGTATGCAAATTTTTATGTTACAATAAATGTGATGTGATGTGATGTGNTGTGNTGT
TARGET=1217,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00825 Ps_contig_mira-and-tgicl-ass_franssen_7815
SEQUENCE=ACCATAACTGCAGGTTTTATGTAAGTATTTACTTATCATTTTTCTTGTTTTCACAAGATACATCACATCACATCACATCACATCACATTTATTGTAACAATAAAAATTTGC
TARGET=60,25
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00826 Ps_contig_mira-and-tgicl-ass_franssen_7825
SEQUENCE=CTCAAAAGAAAGTTAGAAATGCTACTGAGGCTACCAGCAAAGTTTTCTCCCTTGATAGGGAGCCATTCCCCTCTCTGAACGGCCTTCCAGGAGACACTCATCATAGTTACATTGCAGATAAACGACTTGAGAACATCCCATGGAATGGAGTAGATGAGTGGGCTCTAAAGGAGGAAGTGAACGGACTCATTAATGCGACCCTTGTGGAACGAGAGAGAGAAATTGTTCGTCTTTATTATGGACTGGATAAAGAATGTCTTACGTGGGAAGACATAAGTAAACGGAAAGGTTTGTCGAGAGAAAGAGTAAGGCAAGTTGGACTTGTTGCATTGGAGAAACTAAAACATGCCGCAAGGAAGAGGGAGATGGAAGCCATGCTTTTGAAGCACTGATATGATGGACTCATCACAGAGACATGTATATATACAGAACAGAAAGGAAGCATGTACCTAAAAATTTGACTTCCTGATAAAAATAGGCGAAAGAGGACAATATAATGGAACATTGAAGGTTCATAAATCTCCTAAAAAAATTGTCTTTTGGAGTAATTATGTTTTATGAAATATGTTGAAGGATTAATTATATAGATCTCAACTTTCCATTTCAAAATTACGAGGTAACTGTTTCGTAGGAAGACTTGTATTTGTTATCTACAGTTTCACGAGCCAGTTACAATTACGTTGATAGTATGAATAGCATTTAACATGTACTTGTTAATA
TARGET=211,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0738
PRIMER_LEFT_PENALTY=0.038821
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=ATCCCATGGAATGGAGTAGATG
PRIMER_RIGHT_SEQUENCE=CTCGACAAACCTTTCCGTTTAC
PRIMER_LEFT=134,22
PRIMER_RIGHT=297,22
PRIMER_LEFT_TM=60.039
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.0952
PRIMER_LEFT_1_PENALTY=0.060191
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=AAATGCTACTGAGGCTACCAGC
PRIMER_RIGHT_1_SEQUENCE=TCGACAAACCTTTCCGTTTACT
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.0952
PRIMER_LEFT_2_PENALTY=0.060191
PRIMER_RIGHT_2_PENALTY=0.035021
PRIMER_LEFT_2_SEQUENCE=AAATGCTACTGAGGCTACCAGC
PRIMER_RIGHT_2_SEQUENCE=CTCGACAAACCTTTCCGTTTAC
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=297,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=60.035
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.1648
PRIMER_LEFT_3_PENALTY=0.129818
PRIMER_RIGHT_3_PENALTY=0.035021
PRIMER_LEFT_3_SEQUENCE=AAACGACTTGAGAACATCCCAT
PRIMER_RIGHT_3_SEQUENCE=TCGACAAACCTTTCCGTTTACT
PRIMER_LEFT_3=119,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=59.870
PRIMER_RIGHT_3_TM=60.035
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=178
PRIMER_PAIR_PENALTY_4=0.1648
PRIMER_LEFT_4_PENALTY=0.129818
PRIMER_RIGHT_4_PENALTY=0.035021
PRIMER_LEFT_4_SEQUENCE=AAACGACTTGAGAACATCCCAT
PRIMER_RIGHT_4_SEQUENCE=CTCGACAAACCTTTCCGTTTAC
PRIMER_LEFT_4=119,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=59.870
PRIMER_RIGHT_4_TM=60.035
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_00827 Ps_contig_mira-and-tgicl-ass_franssen_7873
SEQUENCE=TTGAGTTGTCTGACGAAGCTGGAGAAATTGTTGTGTTTGAAGTAATTAGGAAGAAGAGTGAGAGAGAATTGAGAGACGTTACCGATGATGAAACTGTTATCGGATTGAGACCAAAAGATGATGGAGTCGGTGGAAGGATCTTCAACCATATCGTAACATTTCGTCAAGAAAGGTGCCACAGAAGAAAAACCATTCTCGCTCGCTGCTTTCACCATAATCGGATACAACGCAAAACAGAGGAACCAACAAAAGAATGTACTTTGAAGCACTCTCTCTCTATAACAGAGTCTAACTAGAAACAAAGACTCATGAACTTCGTTTCACCTTTCCCCTGGACGTCGTTCTTCTACCAGAACTTTTCGTGYC
TARGET=269,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00828 Ps_contig_mira-and-tgicl-ass_franssen_7881
SEQUENCE=TTGTAGTAGTATGGGGGTTTGTATACAGGTGATGGTGGGGATGGTGATTTGTACACGTAAGTTGGTGATGGTGGAGGTGGTGAATTGTACTTGTAAACTGGTGTTGGAGGAGGWGGTAKMAWYGYAGTAGTACRGGGGTTTGTATATAGGTGATGGTGGTGGTGGTGATTTATACACGTATGTTGGTGACGGTGGAGGTGGTGAGTTGTACTTGTAAACCGGTGTTGGAGGAGGAGGTGAGTTATAGTAGKAGTGGGGGTTTGTATGCAGGTGATGGTGGAGGTGGTGACTTGTACACGTTAAGGTTGGTGCCGTCCGGCCTCCGGGCGTCTAGACAAAGGAAGGATGATAGGTGGCGGCGTAGGGAA
TARGET=154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00829 Ps_contig_mira-and-tgicl-ass_franssen_7898
SEQUENCE=CACGGTTGATCCAAGAAGTCACTGCTTCTGCTAATAATGTAGCTCAAGAGACATTCTCTCTTATTAGAACTGTTCGAGTTTATGGAACAGAAGAAGAAGAACAGGGGAGGTACAAATTGTGGCTGGAAAAATTAGCTGACATAAGCTTGCGCCAAAGTGCTGCATATGGTATTTTGGTAATTTTAACTTCAACACTCTTTATCACTCAACCTCTAGGTTATTGCCGTGCTTATTGAGGAT
TARGET=89,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9976
PRIMER_LEFT_PENALTY=1.861571
PRIMER_RIGHT_PENALTY=0.135999
PRIMER_LEFT_SEQUENCE=CACGGTTGATCCAAGAAGTCAC
PRIMER_RIGHT_SEQUENCE=GTCAGCTAATTTTTCCAGCCAC
PRIMER_LEFT=0,22
PRIMER_RIGHT=139,22
PRIMER_LEFT_TM=61.862
PRIMER_RIGHT_TM=60.136
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=140
PRIMER_PAIR_PENALTY_1=2.0818
PRIMER_LEFT_1_PENALTY=1.945787
PRIMER_RIGHT_1_PENALTY=0.135999
PRIMER_LEFT_1_SEQUENCE=ACGGTTGATCCAAGAAGTCAC
PRIMER_RIGHT_1_SEQUENCE=GTCAGCTAATTTTTCCAGCCAC
PRIMER_LEFT_1=1,21
PRIMER_RIGHT_1=139,22
PRIMER_LEFT_1_TM=59.054
PRIMER_RIGHT_1_TM=60.136
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=139
PRIMER_PAIR_PENALTY_2=2.2158
PRIMER_LEFT_2_PENALTY=2.079769
PRIMER_RIGHT_2_PENALTY=0.135999
PRIMER_LEFT_2_SEQUENCE=CACGGTTGATCCAAGAAGTCA
PRIMER_RIGHT_2_SEQUENCE=GTCAGCTAATTTTTCCAGCCAC
PRIMER_LEFT_2=0,21
PRIMER_RIGHT_2=139,22
PRIMER_LEFT_2_TM=61.080
PRIMER_RIGHT_2_TM=60.136
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=2.2264
PRIMER_LEFT_3_PENALTY=1.861571
PRIMER_RIGHT_3_PENALTY=0.364827
PRIMER_LEFT_3_SEQUENCE=CACGGTTGATCCAAGAAGTCAC
PRIMER_RIGHT_3_SEQUENCE=ATGTCAGCTAATTTTTCCAGCC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=141,22
PRIMER_LEFT_3_TM=61.862
PRIMER_RIGHT_3_TM=59.635
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=142
PRIMER_PAIR_PENALTY_4=2.3106
PRIMER_LEFT_4_PENALTY=1.945787
PRIMER_RIGHT_4_PENALTY=0.364827
PRIMER_LEFT_4_SEQUENCE=ACGGTTGATCCAAGAAGTCAC
PRIMER_RIGHT_4_SEQUENCE=ATGTCAGCTAATTTTTCCAGCC
PRIMER_LEFT_4=1,21
PRIMER_RIGHT_4=141,22
PRIMER_LEFT_4_TM=59.054
PRIMER_RIGHT_4_TM=59.635
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_00830 Ps_contig_mira-and-tgicl-ass_franssen_7924
SEQUENCE=GATNTCACATGTTGGCRAGCMMASGTGGAATTTCAGAAAGATTAAAACTTTGATGTTGTTGTTGTTCTTTTTTCAATAATGGAAAACTATTATGAAGCTCTTGTTGTTGATGTTGATTTTGATTATTGAATTGAGTTTGTAGATTTATCATGTGATGATGATGAGAAGATGATGGTGATTGTGATGAGGGTATAATCGGAATTGTGTTATTTGTGATAACTCTTGCACTTTCTTCTGCTAATGCATCACAGAATGCTCTATGAGTTATGAAACTGTCCCTCCTTGAGAAAAGAGTTCCACAGTCACATCTATACTCTCTTGTTCCACATGTTTTAGAATGAGCTTTCCAAT
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00831 Ps_contig_mira-and-tgicl-ass_franssen_7924
SEQUENCE=GATNTCACATGTTGGCRAGCMMASGTGGAATTTCAGAAAGATTAAAACTTTGATGTTGTTGTTGTTCTTTTTTCAATAATGGAAAACTATTATGAAGCTCTTGTTGTTGATGTTGATTTTGATTATTGAATTGAGTTTGTAGATTTATCATGTGATGATGATGAGAAGATGATGGTGATTGTGATGAGGGTATAATCGGAATTGTGTTATTTGTGATAACTCTTGCACTTTCTTCTGCTAATGCATCACAGAATGCTCTATGAGTTATGAAACTGTCCCTCCTTGAGAAAAGAGTTCCACAGTCACATCTATACTCTCTTGTTCCACATGTTTTAGAATGAGCTTTCCAAT
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00832 Ps_contig_mira-and-tgicl-ass_franssen_7925
SEQUENCE=CAACAACAAGAGCTTCATAATAGTTTCCATTATWRAAAAAAGAACAACAACAACATCAAAGTTTTAATCTTTCTGAAATTCCACCTTGGCTTGGCCCACCAAATGTTGGTGAAATCTCATCATCGATATTCTCTCAAACACAACARCAACAACAACATCAAGAAAACCCTAACCCTAATCATGTTCATGTTCATGGGMCCACATTACCTCCTTACCAAACAGTGCCTTCCCCACACATGTCAGCTACTGCATTATTGCAGAAAGCAGCTCAGATGGGTGCAACTATGAATAGAACAGCTTCTGCTTCTTCACCAGCTATGAATGTTAGGTCCCACCAAGTTGATTCTCTCAACAATGTTTCTTCTGGTCATTTTTGGTTTGAATTTGTTATCATCATCACATGAACAACAACAAAACACAACAG
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00833 Ps_contig_mira-and-tgicl-ass_franssen_7926
SEQUENCE=TCCATTTTCCTATTTGACTCTKGCACTATATTATAAGACTCTATCTATTTGCAATTTTAAGTCACCTTTTAARTAATATATTAATAGTTTTTTTATCTATTAAAAGTGGAAATTTTGTTTCACAATTTTCATACTTATTATTATGGCTAGAATCCTAAAACCATCTAAGTGGTTCTCTAACAAAGGTCTTAAACTTAGGTCAAGATCTTCCAATTCATCATCATCATTATGCTCGCCAAAAAATAACAAGAATGAAGATGATATGGGATTAAGAGAGGTTTTCAAWTTACTTTAGATAGTGATGGAGATG
TARGET=215,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00834 Ps_contig_mira-and-tgicl-ass_franssen_7958
SEQUENCE=AACAAACAAGAACTAGTTATTTTTTTATTTTATATAGTTCATTCCTCTTACAGCACACTTAGAAATTGACGATAGACAAAACAAAACAAAACAAACGAAAGGCAACAAAGAAA
TARGET=76,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00835 Ps_contig_mira-and-tgicl-ass_franssen_7968
SEQUENCE=TAGGAAACAGAAAAGGGGTAAAAACAAAATTGATACAATACACACACACATACATTGATTGATAGACAAAAGTGATGATGACTAGTAGTCTTCAGCAGCAAGAGGCTGGTG
TARGET=40,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00836 Ps_contig_mira-and-tgicl-ass_franssen_7983
SEQUENCE=CGAGAAAGAAAACCAAAAACCAAAAACCAAAAACCAGTTTCACAACAAGAAGATGGGAAGAGGTAAGATTGAGATCAAGAGGATTGAAAACTCAAGCAACAGACAAGTTACCTATTCAAAGAGAAAGAACGGTATTCTTAAGAAAGCAAAGGAAATAAGTGTTCTCTGTGATGCTCAAGTTTCTCTTATTCTCTTTGGTGCTTCTGGAAAGATGCATGAATACATCAGTCCCTCCACCACGTTGATTGATGTTCTTGACAGATACCAAAGAGCTTCTGGAAAAACTCTCTGGGATGCTAAAACAATGAGAACCTTAGCAATGAAATTGATAGAATTCAAAAGGAGAATGACAGCATGCAAATTGAACTCAGGCACTTGAAGGGAGAGGACATTACTTCACTGAATTAACAAAGAGCTTATGTCCCTTGAGGATGCCCTTGAAAATGGCCTCACTGGTGTTAGAGACAAAAAGATGGAAGTGCATAGGATGTTCAAGAGAAATGGAAAGATTTTGGAGGATGAAAATAAGGAACTCAACTTTCTTCTGCAACAACATTTGGCATTGGAAGGTGTTGGAAACATGCATGGACAATGGATTTAACCAGATCATAAGGGAATTAGTTTGCTCAATATTATGCCTAAATGGTCATTAATCTTCTAAAAAGAATGTATGTTGAATTAATGGCTGCTACTATATCTAATTATATATATATATAGTGTGCTCTAAGTCGTTGGGTTGGTGAACTTAATTGTAATTTCTATTTCTGATTTCAGTAGGGTTTGTTGATTATGTATCATTGGAGACCTTTGAGTCTAAACAATATCCAAAAACTTGTCATTGAAGGTGAAGTTTATTGGCTTTGATTTGACTATTAAATAAGTGGTGTTTTCTG
TARGET=703,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6442
PRIMER_LEFT_PENALTY=0.501869
PRIMER_RIGHT_PENALTY=0.142283
PRIMER_LEFT_SEQUENCE=TGGAAGTGCATAGGATGTTCAA
PRIMER_RIGHT_SEQUENCE=AATTAAGTTCACCAACCCAACG
PRIMER_LEFT=473,22
PRIMER_RIGHT=750,22
PRIMER_LEFT_TM=60.502
PRIMER_RIGHT_TM=60.142
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=278
PRIMER_PAIR_PENALTY_1=0.6850
PRIMER_LEFT_1_PENALTY=0.542697
PRIMER_RIGHT_1_PENALTY=0.142283
PRIMER_LEFT_1_SEQUENCE=ATGGAAGTGCATAGGATGTTCA
PRIMER_RIGHT_1_SEQUENCE=AATTAAGTTCACCAACCCAACG
PRIMER_LEFT_1=472,22
PRIMER_RIGHT_1=750,22
PRIMER_LEFT_1_TM=59.457
PRIMER_RIGHT_1_TM=60.142
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.9407
PRIMER_LEFT_2_PENALTY=0.798404
PRIMER_RIGHT_2_PENALTY=0.142283
PRIMER_LEFT_2_SEQUENCE=CATGGACAATGGATTTAACCAG
PRIMER_RIGHT_2_SEQUENCE=AATTAAGTTCACCAACCCAACG
PRIMER_LEFT_2=583,22
PRIMER_RIGHT_2=750,22
PRIMER_LEFT_2_TM=59.202
PRIMER_RIGHT_2_TM=60.142
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=1.1246
PRIMER_LEFT_3_PENALTY=0.501869
PRIMER_RIGHT_3_PENALTY=0.622772
PRIMER_LEFT_3_SEQUENCE=TGGAAGTGCATAGGATGTTCAA
PRIMER_RIGHT_3_SEQUENCE=ATTAAGTTCACCAACCCAACGA
PRIMER_LEFT_3=473,22
PRIMER_RIGHT_3=749,22
PRIMER_LEFT_3_TM=60.502
PRIMER_RIGHT_3_TM=60.623
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=1.1379
PRIMER_LEFT_4_PENALTY=0.995624
PRIMER_RIGHT_4_PENALTY=0.142283
PRIMER_LEFT_4_SEQUENCE=CATTGGAAGGTGTTGGAAACAT
PRIMER_RIGHT_4_SEQUENCE=AATTAAGTTCACCAACCCAACG
PRIMER_LEFT_4=560,22
PRIMER_RIGHT_4=750,22
PRIMER_LEFT_4_TM=60.996
PRIMER_RIGHT_4_TM=60.142
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_00837 Ps_contig_mira-and-tgicl-ass_franssen_7992
SEQUENCE=CAAAATCAAAATCATCATCACACTTCTTAATCAAAWTCCTAACTCAGATGGTTCAAACCCAAATGAGTTTTTTGAGCTTGGATAGTCACCATGATGGTGGAAGGAAAATGGACAAGTTTAAGAGAAGTTTCACTTTACCTACAAGAGTTGCTTCATCATCTTCATCTACTTCAATGGATCATCATCAACAACAACAAGGTGTGGAGTATAGGAATTCAGAAGGTGGTATGTACTCAGATGTTATGGAGACCTTTTTGGAGTGAATGTTCTTGAAAAGTATGATTTAGAATTTGTATCTAGGAAAAGCTAATGATGTGTTTCTTTTTCATGTGTTTTGTTTTTTCACTATTGTATCTTTGTTGTTTTCTTTATGTTATT
TARGET=186,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00838 Ps_contig_mira-and-tgicl-ass_franssen_7998
SEQUENCE=TCGGGKACTCTTTCCACTTTTTCATTTCATTCTTTTCCATGTAAGAGATACAAGAGAACAAAACAGTAGCAGATTGATTCTAAACAACAAAGAAGAAAAGCATCACATCACGTAACTGCCAACAACAATGGCGTCTCCTTCTTCTTCTTCTTCTTCTTCCCGCGTCCTGTTATTTCTCATGCTGTTTCTGACTGCATCTGCCTCCGATGATGTGATGCGCGATGACGAAAGAGCTCCAAAATCCGAATCATGCAATAACCCCTTCCAATTGGTTAAGGTGGAAATTGG
TARGET=138,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00839 Ps_contig_mira-and-tgicl-ass_franssen_7999
SEQUENCE=AACAGAGAGAAGAGTGAAGAAGAAGAATAGCAGTGGCAGTAACTAACTACTCTTTCCACTTTTTCATTTCATTCTTTTCCATGTAAGAGATACAAGAGRACAAAACAGTAG
TARGET=16,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00840 Ps_contig_mira-and-tgicl-ass_franssen_8001
SEQUENCE=TCTGCTCCGTCTACGGCGACGACTTTTGTCGGTAAGCTTCTCCCTTCGTCTCCTGCGTTTCAGTTCTTCTTCTTCAATGTTCTTCCGCGTTTGTGTCGCATTTTCAGAACT
TARGET=64,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.1672
PRIMER_LEFT_PENALTY=2.826973
PRIMER_RIGHT_PENALTY=0.340219
PRIMER_LEFT_SEQUENCE=GTCTACGGCGACGACTTTTG
PRIMER_RIGHT_SEQUENCE=GTTCTGAAAATGCGACACAAAC
PRIMER_LEFT=8,20
PRIMER_RIGHT=109,22
PRIMER_LEFT_TM=60.827
PRIMER_RIGHT_TM=59.660
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=4.9428
PRIMER_LEFT_1_PENALTY=4.602532
PRIMER_RIGHT_1_PENALTY=0.340219
PRIMER_LEFT_1_SEQUENCE=CGTCTACGGCGACGACTTTT
PRIMER_RIGHT_1_SEQUENCE=GTTCTGAAAATGCGACACAAAC
PRIMER_LEFT_1=7,20
PRIMER_RIGHT_1=109,22
PRIMER_LEFT_1_TM=62.603
PRIMER_RIGHT_1_TM=59.660
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=103
PRIMER_PAIR_PENALTY_2=6.8960
PRIMER_LEFT_2_PENALTY=4.602532
PRIMER_RIGHT_2_PENALTY=2.293496
PRIMER_LEFT_2_SEQUENCE=CGTCTACGGCGACGACTTTT
PRIMER_RIGHT_2_SEQUENCE=CTGAAAATGCGACACAAACG
PRIMER_LEFT_2=7,20
PRIMER_RIGHT_2=106,20
PRIMER_LEFT_2_TM=62.603
PRIMER_RIGHT_2_TM=60.293
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=100
PRIMER_PAIR_PENALTY_3=7.5151
PRIMER_LEFT_3_PENALTY=2.826973
PRIMER_RIGHT_3_PENALTY=4.688082
PRIMER_LEFT_3_SEQUENCE=GTCTACGGCGACGACTTTTG
PRIMER_RIGHT_3_SEQUENCE=TCTGAAAATGCGACACAAAC
PRIMER_LEFT_3=8,20
PRIMER_RIGHT_3=107,20
PRIMER_LEFT_3_TM=60.827
PRIMER_RIGHT_3_TM=57.312
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=7.5151
PRIMER_LEFT_4_PENALTY=2.826973
PRIMER_RIGHT_4_PENALTY=4.688082
PRIMER_LEFT_4_SEQUENCE=GTCTACGGCGACGACTTTTG
PRIMER_RIGHT_4_SEQUENCE=GTTCTGAAAATGCGACACAA
PRIMER_LEFT_4=8,20
PRIMER_RIGHT_4=109,20
PRIMER_LEFT_4_TM=60.827
PRIMER_RIGHT_4_TM=57.312
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=102
=
PRIMER_SEQUENCE_ID=SSR_00841 Ps_contig_mira-and-tgicl-ass_franssen_8034
SEQUENCE=GTTTGTTCGTTTATTTATTTGGTCATATGTGTGTGTGTGTGTGAAAGTACGAGTGAAATGAGTGGATATAGACATTGGTCATTAGAGTTACTCCCTGCTTTTGAAGGATTTTGATTTGATGTATGTTGCATGCAGTCRTGCTAAGTTAATAACTCCAAGTTGGTCACGATACGTCCAATTGTTTAACTTTTTTTTTAATGTTAATTTTTATTTAATTTAATAAAAAATTTATTTANGA
TARGET=28,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00842 Ps_contig_mira-and-tgicl-ass_franssen_8061
SEQUENCE=GATTTTTTATGTGTTTTGACTTTAGCTTGTAAAATCTTATGCTTTTCAACTTCCTGCACCAGAACCTGCTTCATAGGTTAGGGTATTAGGCATCATATCTGACTTCATTTTCTTGTATGTGATAGCATATGACACCCTTGTGCTAACTAATTGAGAAAGATCATTCTTCTCCTTATTTGATAGTAGTTGCAGTGCCGCCAGTCTTATGCTTGATGGGGATAGAATATGTAATAATGGAGAAATTAAGTCTTTAACAAAAGAAGTAGTTGTAGCATGATCAAATAGGAGCATATGGGTGCTTAAGAGGATGCGATGAGTGGGATGACCTAAATTTTACCCAAAACCTTCCTTCCTTTTCCCTTTTGAATTTACCAAAAACCCTAATTAATTATTTGGTGGTAATTATTTAATAGTAAATTAAAGTAAATTTAAGGAAGTTAA
TARGET=344,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00843 Ps_contig_mira-and-tgicl-ass_franssen_8062
SEQUENCE=TYCAGAATCGTTCTTCTTCTTTCCCCTCAAACTCCACCAAAACTTGATCAAATTTCCATAACCATTTCTCACAAAAGACCGCCATAACCGTTTCCTCACTCCACCTTCCATTTCTCCTTAAATCCTTCAATGATCCTCAATCTTTAATAACGAACCACCACCACATGCTTCCTCCACCTGCATAATCAAATCGTCGTCGCCGCCACATTCGTTCCGCGACAACCACGGTGAATCCAGACAAAATAGAGTGATAATACCAAGTCGTGATCTACGGTAGATCGTAGCAGAAGTTTGTAACTGAAGGACAAGTGGAGGATAGATGGTTGCAGAACCGAATCGTGCAGGATACCGGATTTTCGAGTTCGTCTTCGCCGTCGCCGGTAAGAACGAGCCGTAGTAGCGATCTGGTTTTTTGTGTGTGTGGAGTATTTGCTTCCCGTTTCAATTAGGAGAGTTTAAGGTCGCGTAGAATGGTGTAACGCTTCAAGGTGAACCGCCGCCGCCGATGGTGAAAGCCGACACGGCTAGGCTTTTACGTTGTCTCACGTTTTCTCTTTGTTGAGTTTCCCCGNGTACTCTGCGTTGACTACCCA
TARGET=414,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00844 Ps_contig_mira-and-tgicl-ass_franssen_8062
SEQUENCE=TYCAGAATCGTTCTTCTTCTTTCCCCTCAAACTCCACCAAAACTTGATCAAATTTCCATAACCATTTCTCACAAAAGACCGCCATAACCGTTTCCTCACTCCACCTTCCATTTCTCCTTAAATCCTTCAATGATCCTCAATCTTTAATAACGAACCACCACCACATGCTTCCTCCACCTGCATAATCAAATCGTCGTCGCCGCCACATTCGTTCCGCGACAACCACGGTGAATCCAGACAAAATAGAGTGATAATACCAAGTCGTGATCTACGGTAGATCGTAGCAGAAGTTTGTAACTGAAGGACAAGTGGAGGATAGATGGTTGCAGAACCGAATCGTGCAGGATACCGGATTTTCGAGTTCGTCTTCGCCGTCGCCGGTAAGAACGAGCCGTAGTAGCGATCTGGTTTTTTGTGTGTGTGGAGTATTTGCTTCCCGTTTCAATTAGGAGAGTTTAAGGTCGCGTAGAATGGTGTAACGCTTCAAGGTGAACCGCCGCCGCCGATGGTGAAAGCCGACACGGCTAGGCTTTTACGTTGTCTCACGTTTTCTCTTTGTTGAGTTTCCCCGNGTACTCTGCGTTGACTACCCA
TARGET=494,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00845 Ps_contig_mira-and-tgicl-ass_franssen_8064
SEQUENCE=CGGGGATGCATTACAATTCCAATCCCAATTTCTTCTATCTTTCTCTTTCTTGTTCCTCTCCTTGTTAACACCAAAAAAACCAAACAAGAATCATGGTTGTTTTGTCTAAACCATCCTTAAACAATTTCCTTCTTCCCAAATCACCATGCAAACCAACAACAACAACAACTATGTTCAATGGAATACCTATCATCGACGTCACAGACCCAGAAGCAAAGACANAATTGCAACGCTTAGAAGGAGTTGTTCTA
TARGET=154,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5884
PRIMER_LEFT_PENALTY=0.522051
PRIMER_RIGHT_PENALTY=0.066354
PRIMER_LEFT_SEQUENCE=ATTTCCTTCTTCCCAAATCACC
PRIMER_RIGHT_SEQUENCE=TTNTGTCTTTGCTTCTGGGTCT
PRIMER_LEFT=123,22
PRIMER_RIGHT=223,22
PRIMER_LEFT_TM=60.522
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=1.1766
PRIMER_LEFT_1_PENALTY=0.522051
PRIMER_RIGHT_1_PENALTY=0.654586
PRIMER_LEFT_1_SEQUENCE=ATTTCCTTCTTCCCAAATCACC
PRIMER_RIGHT_1_SEQUENCE=CAATTNTGTCTTTGCTTCTGGG
PRIMER_LEFT_1=123,22
PRIMER_RIGHT_1=226,22
PRIMER_LEFT_1_TM=60.522
PRIMER_RIGHT_1_TM=60.655
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=104
PRIMER_PAIR_PENALTY_2=1.2377
PRIMER_LEFT_2_PENALTY=0.522051
PRIMER_RIGHT_2_PENALTY=0.715687
PRIMER_LEFT_2_SEQUENCE=ATTTCCTTCTTCCCAAATCACC
PRIMER_RIGHT_2_SEQUENCE=ATTNTGTCTTTGCTTCTGGGTC
PRIMER_LEFT_2=123,22
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=60.522
PRIMER_RIGHT_2_TM=59.284
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=102
PRIMER_PAIR_PENALTY_3=1.3014
PRIMER_LEFT_3_PENALTY=0.522051
PRIMER_RIGHT_3_PENALTY=0.779358
PRIMER_LEFT_3_SEQUENCE=ATTTCCTTCTTCCCAAATCACC
PRIMER_RIGHT_3_SEQUENCE=CCTTCTAAGCGTTGCAATTNTG
PRIMER_LEFT_3=123,22
PRIMER_RIGHT_3=240,22
PRIMER_LEFT_3_TM=60.522
PRIMER_RIGHT_3_TM=60.779
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.9500
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=1.3145
PRIMER_LEFT_4_PENALTY=0.522051
PRIMER_RIGHT_4_PENALTY=0.792480
PRIMER_LEFT_4_SEQUENCE=ATTTCCTTCTTCCCAAATCACC
PRIMER_RIGHT_4_SEQUENCE=GCAATTNTGTCTTTGCTTCTGG
PRIMER_LEFT_4=123,22
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=60.522
PRIMER_RIGHT_4_TM=60.792
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_00846 Ps_contig_mira-and-tgicl-ass_franssen_8067
SEQUENCE=CAACTCACAATAATGTGAACCGCGCATGCAAATGCAATAGAGTTGAGGTGTATCTATCAACTACAATAATGCCGACTATATATATACAACTAGTCGAAACAAACAGTACAATTTTATTCAAGAAATGACAATTTATGAATCTCAGTTCAACCTGTGGAAAAATGCAATGCCAATTGAATTCCCAAGAGAGCTATGTTATAACAGATACCTCAACTTATGTGCAGCTGATCCTTTGATCGCATCAAATTGTTAAGTACAATCTCCAATGGAAGAAATTACCTAGATGGAACCAAGTGAATTAAAAAGACTACTACACTCTGATCAGAACTCAAGAATTTGTCACATCCCAACTCTTTTTCATGAATGGAGGCAAATCCGCTTCCAACTCAAGTGCTTTAACCGCTGAAAGGTCATAACTTAAAGCTGATAGTGATTGCACATTTTGCAGTGCTTGAGATATATCAGGCAACACTATTTGCTTGCAATGAAGATGTAAATGAGGACGCTTGTCAGAGATGCTCCCCTTGTTCAAGTTAAGGCCAAACGGTAGTGCCTCTTCCTTCAGAAGCTCTTCAGTTGAGTCCTCCGGATTAGACAAATCAAACTGTCCCCACTTCTTATGAGCTTGCCATCCATACTTGTAGTCCCCAACTATTGGTGTACCTAACACCTCGGCACAGTGGACTCGGAGCTGGTGTTTTCTACCGGTTAAGGGGGAAAGCTCCAACCACGTATAACCATGAGATGATGATGCAATCACCCGGTACTCTGTAATTGCATGTTGGGGTGACAGTGATGTAGAATTGTCAACGATAGTTATTCGATCAGATTTTCCGTTGTCAACCACCACCTTACCCAGTGGAGCAGTAACCAAACCCCCTGAACGTCTAGGACATCCGAGCACCAGTGCCCAATACTTTCTTTGTAGGATTCTCTTTTC
TARGET=77,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3598
PRIMER_LEFT_PENALTY=0.264300
PRIMER_RIGHT_PENALTY=0.095538
PRIMER_LEFT_SEQUENCE=TGCAAATGCAATAGAGTTGAGG
PRIMER_RIGHT_SEQUENCE=CAAAGGATCAGCTGCACATAAG
PRIMER_LEFT=26,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=60.264
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.5131
PRIMER_LEFT_1_PENALTY=0.264300
PRIMER_RIGHT_1_PENALTY=0.248781
PRIMER_LEFT_1_SEQUENCE=TGCAAATGCAATAGAGTTGAGG
PRIMER_RIGHT_1_SEQUENCE=TTTTCCACAGGTTGAACTGAGA
PRIMER_LEFT_1=26,22
PRIMER_RIGHT_1=160,22
PRIMER_LEFT_1_TM=60.264
PRIMER_RIGHT_1_TM=59.751
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=0.5284
PRIMER_LEFT_2_PENALTY=0.264300
PRIMER_RIGHT_2_PENALTY=0.264143
PRIMER_LEFT_2_SEQUENCE=TGCAAATGCAATAGAGTTGAGG
PRIMER_RIGHT_2_SEQUENCE=ATCAAAGGATCAGCTGCACATA
PRIMER_LEFT_2=26,22
PRIMER_RIGHT_2=236,22
PRIMER_LEFT_2_TM=60.264
PRIMER_RIGHT_2_TM=59.736
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.5426
PRIMER_LEFT_3_PENALTY=0.264300
PRIMER_RIGHT_3_PENALTY=0.278329
PRIMER_LEFT_3_SEQUENCE=TGCAAATGCAATAGAGTTGAGG
PRIMER_RIGHT_3_SEQUENCE=GGATCAGCTGCACATAAGTTGA
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=230,22
PRIMER_LEFT_3_TM=60.264
PRIMER_RIGHT_3_TM=60.278
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=0.6569
PRIMER_LEFT_4_PENALTY=0.264300
PRIMER_RIGHT_4_PENALTY=0.392624
PRIMER_LEFT_4_SEQUENCE=TGCAAATGCAATAGAGTTGAGG
PRIMER_RIGHT_4_SEQUENCE=GCATTTTTCCACAGGTTGAACT
PRIMER_LEFT_4=26,22
PRIMER_RIGHT_4=164,22
PRIMER_LEFT_4_TM=60.264
PRIMER_RIGHT_4_TM=60.393
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_00847 Ps_contig_mira-and-tgicl-ass_franssen_8069
SEQUENCE=CCTTGAAGCATTTTTTGATCTTCTTCCTGATTCTGATGACGAGGGTGTGGACACTGAAGAAAAACAAAGTCTTTGATTGGGTGTAGTACACAATAGGCTGTACTTTCTTTCTTTGATTGTTTCTTTCTTTTATTGGATATCAGTAATTTAGTTTCCAAAAAAGAAAGGTGGCAAATATATAGTGATAATAGGTTGCTCCATGTTAGTTAGCACTTCTAATCAGCTCATTCATCCTCATTAATTGACATTCAATTGGAGGTCTCAAGTCTCAACAAATTCTTATAATGAATTGATAATATTTACACTGTCGCGAGTACATTCAAGGCAATTTAATACAATTTGGTAGGGAGTAGCGACGACTAGTTTWATATATAGAGTATGTATATGCTGTTGAAGATTGTATTCTTCAGTTTGCGATCAACTAGGTTTATGAGTATTGGATGTATTCATTT
TARGET=103,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00848 Ps_contig_mira-and-tgicl-ass_franssen_8080
SEQUENCE=AGTATAATAAGCAATTTATAATCTTTAACACCAGCTTTGAACCCCAAMCCTTTGTCTTTTCGTTTCTGTTTGTGGTGACATAAATCTCCCCCTACAACGCATGAATTAAGAATTATGGCAACAACAACAAGGTCTA
TARGET=119,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00849 Ps_contig_mira-and-tgicl-ass_franssen_8089
SEQUENCE=AGGTTTCTATAAAAGTGTTGATTACGTGATAACAATTAGTGACAATATGTCCAAATTCGAAACAAATTTGACATTGCATATTACCATGGCTACCACGACCACCATGATAGCTTGTATGTCCACCTCTTCCAAAACCATAGCTAGAAGGTGTAGAAGAAGAGTCATAAAAGGATACATATGTTATGTGCATTCAAATCATGTGTACCAAAGAAAGAATCTAAAACAGGTTTTGACTGAGTGAGATTGATTGAAACATTGTCAATAATTAAGATCTTCTTGAATCTTTCAGAACGCAATTCATGATCATGCAACAGAGCCTCCACTTCCTCAAGCTAAATAGACTCAAACTTGCTTTCTATAACCTGAAATAACTGGTCCATACTCATGGGAAAGACCTTCAAGCACAACATCAATGTATTGATGTGAAGGAATTGAATCTCCTACCAATGCAAGAGTCAATTAGAGCCTTTACACGAAGCATATAGTCTTGTACCGATAAATCCCTAAAAACTATGGCGCGAAGCTCAGATTGCAACTAACGAGCCCGAGTCATATTACAATCTCTGTTTAAAATATCATCATTTCAACCCTTCACTAAGCCCAGGTGGCATTCCTAAGCTCTGTGCAAGACCATCCATCCTTTCCTTCATGGCCTGGACACTCTTCTGGTGTGCTTCCTTGTATGCTTCAGTGATTAAAAGCGATAGTTTTTCTGATCCCAATTCCATAGCTGCCTCAGTAATTTCTGTCCTTAAGGGTTGCTGGTTTCCTGTCAGAGTTACCTTTACTAACTCACCCTCACAATAACCATCAAACTCAGTCGCTGCAAGTTCTTTCTGCACCCTCACTGCTTCAACTTGGACAACCATTTGTGCCTTCTTCAAAGTCTCAAAGAGGTTTTGCATGTTTCCAAGAATTCCTGCCTTAGAAGGTGCATCATCATCACTTTTCTCATTACCATCTTTTCTCCCTCCAAATAATCCATACGATCGAATACATCTTTGGCCATGTCCAACTTTTGAACAACCGCGCCGCGAAAGAATCTTCATGTCAACTCCTTCTGAGCGAAGAGTTGATGAAAAGGTACTGTGATTTTTGCTGCTACAGAATCCGATTGCGTTTGAGAGAGAGACGACCGACGCCATTGCCTTCCACACCCAAGGAGCAGCGATAACCGACTGCCGGGGATAGATGTAACTGGAAGATTGTCGCAAGAGSACACCGCAATMACCCCCGA
TARGET=1123,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00850 Ps_contig_mira-and-tgicl-ass_franssen_8099
SEQUENCE=AGCTCTAGCTTGTTCTAAAGAAGAAGAACTTAGCACAACAAACNCTTAAGGAGTAATCATTTTGTGCTGCATGCATGCATGCTTCCATTGACAGTTTTTTGCGGTCGAGAATTCTGCATAATCTTTTCCTTTCACTCTTGTTGAGTTCTGGGTGAGCCTTGAGATAAA
TARGET=68,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.6286
PRIMER_LEFT_PENALTY=3.431623
PRIMER_RIGHT_PENALTY=0.197024
PRIMER_LEFT_SEQUENCE=GAAGAACTTAGCACAACAAACNC
PRIMER_RIGHT_SEQUENCE=CACCCAGAACTCAACAAGAGTG
PRIMER_LEFT=22,23
PRIMER_RIGHT=153,22
PRIMER_LEFT_TM=57.568
PRIMER_RIGHT_TM=59.803
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.0250
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=4.0595
PRIMER_LEFT_1_PENALTY=3.431623
PRIMER_RIGHT_1_PENALTY=0.627863
PRIMER_LEFT_1_SEQUENCE=GAAGAACTTAGCACAACAAACNC
PRIMER_RIGHT_1_SEQUENCE=CCCAGAACTCAACAAGAGTGAA
PRIMER_LEFT_1=22,23
PRIMER_RIGHT_1=151,22
PRIMER_LEFT_1_TM=57.568
PRIMER_RIGHT_1_TM=59.372
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.0250
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=4.0616
PRIMER_LEFT_2_PENALTY=3.431623
PRIMER_RIGHT_2_PENALTY=0.629991
PRIMER_LEFT_2_SEQUENCE=GAAGAACTTAGCACAACAAACNC
PRIMER_RIGHT_2_SEQUENCE=TATCTCAAGGCTCACCCAGAAC
PRIMER_LEFT_2=22,23
PRIMER_RIGHT_2=165,22
PRIMER_LEFT_2_TM=57.568
PRIMER_RIGHT_2_TM=60.630
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.0250
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=4.3806
PRIMER_LEFT_3_PENALTY=3.431623
PRIMER_RIGHT_3_PENALTY=0.948996
PRIMER_LEFT_3_SEQUENCE=GAAGAACTTAGCACAACAAACNC
PRIMER_RIGHT_3_SEQUENCE=GATTATGCAGAATTCTCGACCG
PRIMER_LEFT_3=22,23
PRIMER_RIGHT_3=122,22
PRIMER_LEFT_3_TM=57.568
PRIMER_RIGHT_3_TM=60.949
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.0250
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=4.4442
PRIMER_LEFT_4_PENALTY=3.431623
PRIMER_RIGHT_4_PENALTY=1.012540
PRIMER_LEFT_4_SEQUENCE=GAAGAACTTAGCACAACAAACNC
PRIMER_RIGHT_4_SEQUENCE=CTCACCCAGAACTCAACAAGAG
PRIMER_LEFT_4=22,23
PRIMER_RIGHT_4=155,22
PRIMER_LEFT_4_TM=57.568
PRIMER_RIGHT_4_TM=58.987
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.0250
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_00851 Ps_contig_mira-and-tgicl-ass_franssen_8131
SEQUENCE=AAAGCCATTACTTGACTTGTTCCTTCCACTGAACTTGCTATATAGAATCCTTGTGCTTTCCCTACTAATCCTGATCCCAACTCTTCCCCTTCTGTTAATTCATCATCAAACACTGTCATTGTTCCAAACATCAACTCCTGCACCGTCATTCCCTGTGGTATCTGATTCATATTCGTCAACCGGAAAATTGTTGTTCCCGTTGTTGTTGTTATTGTTATTGAAGACGTTTCCCGTGTTTCCGCTGAGTCCTGTTAGGAAAGGGAGGTTATTGTTGTTTAAG
TARGET=199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1659
PRIMER_LEFT_PENALTY=0.040146
PRIMER_RIGHT_PENALTY=0.125755
PRIMER_LEFT_SEQUENCE=TCTGATTCATATTCGTCAACCG
PRIMER_RIGHT_SEQUENCE=AACCTCCCTTTCCTAACAGGAC
PRIMER_LEFT=160,22
PRIMER_RIGHT=266,22
PRIMER_LEFT_TM=59.960
PRIMER_RIGHT_TM=59.874
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=0.2628
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.254567
PRIMER_LEFT_1_SEQUENCE=TTGTTCCAAACATCAACTCCTG
PRIMER_RIGHT_1_SEQUENCE=CTCCCTTTCCTAACAGGACTCA
PRIMER_LEFT_1=118,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=59.745
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.2628
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.254567
PRIMER_LEFT_2_SEQUENCE=TTGTTCCAAACATCAACTCCTG
PRIMER_RIGHT_2_SEQUENCE=TCCCTTTCCTAACAGGACTCAG
PRIMER_LEFT_2=118,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=59.745
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=0.3329
PRIMER_LEFT_3_PENALTY=0.207104
PRIMER_RIGHT_3_PENALTY=0.125755
PRIMER_LEFT_3_SEQUENCE=TTCCCTACTAATCCTGATCCCA
PRIMER_RIGHT_3_SEQUENCE=AACCTCCCTTTCCTAACAGGAC
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=59.793
PRIMER_RIGHT_3_TM=59.874
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=0.3329
PRIMER_LEFT_4_PENALTY=0.207104
PRIMER_RIGHT_4_PENALTY=0.125755
PRIMER_LEFT_4_SEQUENCE=TCCCTACTAATCCTGATCCCAA
PRIMER_RIGHT_4_SEQUENCE=AACCTCCCTTTCCTAACAGGAC
PRIMER_LEFT_4=58,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=59.793
PRIMER_RIGHT_4_TM=59.874
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_00852 Ps_contig_mira-and-tgicl-ass_franssen_8137
SEQUENCE=GAAGTGAATCCTCATCCCAAGCAAACTCCCACTTACCATTCTCCTCTTTCCTCTGCGCCTTCACCGGAAGCTGGTTACCAACAATAATCATCCTTTCTTGAGAAACAGACGAAGGAGCATCAGAGCCAACACTGTTGTTTGTTTCATCATCTAATTCAGACAACACTCCAGCCACAGTTGCAACTCTGGGAAGCCTTCTCTTCTCCCGACTGAAAGACGACGGCGAACCACATGAAGTAAGATCTAACAAATTGGAATATGACCTTGAAGCCATTTTTCTCACTCACTCACTCACAGTCACAATCCTTGAATGAATGTTAGTTGGAGAAGAGACWATTAAACCAAAGATTCTTGTTCTCCGTCAAAATCACNCACTCACTCAACTTAACCCCCGAC
TARGET=279,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00853 Ps_contig_mira-and-tgicl-ass_franssen_8174
SEQUENCE=ATTGCATTTCTAACAGGCAAGAATACAGCATCTATAGTATAACTTCTATCTGAATGTTGTTAGTGTTTTTATACGATATCCCCACTTTCTCTCAATTTGTAATTCAATACACAAATTAATTGGTTCTGTTAGGTTAGTCTTATTATGTTGTGTATTATTCAATACTCAGGGATTAATATAGGTAGATTATTCATATAATTAGTCCAAAAAGTTATTTAATCTACATTGAATATATATTGGCAGATTATTCATTATTAATTTAGTCCAAAAAGTTATCCCGCTATCCCATTTTTGGGGTCGGACGCTCCGCTCCGCTCTCTGGGATTAATAACTAGAATCTAACATGGTATCACTTTGCTTAGGTTGTGATTCTTAGGKTGTTTCSGTGGATGCAACACTACAACCGGTGCAATCACCGGTGGCTTCCACTCATGTTTCTGATTCTCAGTGCGTTATGTCGAGGATTAAGTGTCTTCACTTCTCGCTCAACCCGG
TARGET=303,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00854 Ps_contig_mira-and-tgicl-ass_franssen_8191
SEQUENCE=GTTCAATATGCATTGACAATCAAACTTATTTGGATTCATTTCAATTTCTCCGGCTGGATCTGGTGATCATGATGATGATGATAGGTAGTAGGGCTGAGGTTGGCTTTGGTGTAGCTGCTA
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00855 Ps_contig_mira-and-tgicl-ass_franssen_8202
SEQUENCE=AGAGGAGAAAGAAAGGGAAAAGTACGTGAATATAAAATAACCCACTTCATGCATTCAATTTGAAATATACTTGTGTCCTCCCAACTTCACCCATTCATTCATTGCAGATTAACCTAATTTAACAKWWMARAATTAGTTGCTTGTTTTTATTGCTTGCAGTTYTCACTTCATTCAACAACTACATGGGGAATTGTGTGGTTAAGTCTCACTCTCATGTGAAAGAGGATGATTCTCTTCATCAGGACGTCGAATTTGCTTCTGGGAAGGTTCATCTTATTAATACCAAAGAATCTTGGGACCAAAAGTTAGAACAAGCAAGAGGAGATGGAAAAATGGTTATTGCAAATTTCAGTGCGACGTGGTGTGGTCCTTGTAAGATGATTGCACCATATTATTGCGAGTTATCCGAGCACTATCCGTCCATTATGTTCTTACTAATTGATGTCGATGAACTAATTGACTTTAGCACGTCGTGGGACATTAAAGCCACGCCGACGTTCTTTTTTCTTAGAGATGGACAAGAGATTGACAAACTTGTAGGAGCCAACAAGCCAGAGCTGGAGAGGAAGATTGCTAGTGTTAATGTGGTTCCCCGACATCAATAGCGATTCATGAAAGTTATTGCTCAACTCTTCTTCTCTCATCAGAGCCTGCAGTGATATGTATTCAATGTGTAATCCTACAACTTTGTCGCTGTATTTTTCTTTGTAAGTAAATATGCAAACATTTCAAACAAATATCTTGTATTCAATTGATATCATTGTAGTTAAAAGAAGTTTATTTCTGATAWATAAAGAATTGTTGAAGTTTT
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00856 Ps_contig_mira-and-tgicl-ass_franssen_8204
SEQUENCE=CTATGGGTTTTACTAGTGCTAGTGAGCTCCATGCCCAGCGAGAATCTATTATTCAGATAACCACTGGGTCAAGAGAGCTTGACAAGATATTAGAAGGTGGAATTGAGACGGGTTCTATCACTGAAATGTATGGTGAATTTAGATCTGGGAAGACTCAGTTGTGTCACACTCTCTGTGTCACTTGTCAATTGCCACTAGATCAAGGAGGAGGTGAGGGTAAAGCTATGTACATAGATGCTGAGGGAACATTTAGACCTCAGCGACTCTTACAGATAGCAGACAGGTTTGGACTGAATGGTGCTGATGTATTGGAAAATGTCGCTTATGCTAGAGCTTATAACACGGATCATCAATCACGGCTTCTGCTTGAAGCAGCTTCGATGATGGTTGATACAAGGTTTGCTCTAATGATAATAGACAGTGCTACTGCTCTCTATAGGACAGATTTTTCCGGAAGAGGGGAGCTTTCAGCCCGGCAAATGCATTTAGCAAAGTTCCTGAGAAGCCTTCAGAAATTAGCAGATGAGTTCGGTGTGGCTGTTGTCTTAACAAACCAAGTAGTCTCACAAGTAGATGGTTCTGCAATGTTTGCTGGACCCCAAACCAAACCTATTGGAGGCAACATTATGGCTCATGCAACTACAACAAGGTTAGCTCTCAGGAAAGGAAGAGGAGAAGAGCGAATCTGTAAAGTGATTAGTTCTCCTTGTTTGGCTGAAGCCGAAGCAAGATTTCAGATATTAGGCGAAGGAGTTTCAGATGTTAAAGACTAACCATTTTTAGCATTGTCTGAAACATTGTAATTTTTTGTTTGTTTGTTTGTGTTCTTTTTGCTAGTTTGTAAAAGATGATTTGCAAGTATCCATGTCTAGTTTTGCAATAA
TARGET=807,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.7598
PRIMER_LEFT_PENALTY=0.012045
PRIMER_RIGHT_PENALTY=2.747724
PRIMER_LEFT_SEQUENCE=AAGGAAGAGGAGAAGAGCGAAT
PRIMER_RIGHT_SEQUENCE=GCAAAACTAGACATGGATACTTGC
PRIMER_LEFT=663,22
PRIMER_RIGHT=877,24
PRIMER_LEFT_TM=59.988
PRIMER_RIGHT_TM=59.252
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=215
PRIMER_PAIR_PENALTY_1=2.8258
PRIMER_LEFT_1_PENALTY=0.078029
PRIMER_RIGHT_1_PENALTY=2.747724
PRIMER_LEFT_1_SEQUENCE=AGTTCTCCTTGTTTGGCTGAAG
PRIMER_RIGHT_1_SEQUENCE=GCAAAACTAGACATGGATACTTGC
PRIMER_LEFT_1=698,22
PRIMER_RIGHT_1=877,24
PRIMER_LEFT_1_TM=59.922
PRIMER_RIGHT_1_TM=59.252
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=2.9844
PRIMER_LEFT_2_PENALTY=0.236697
PRIMER_RIGHT_2_PENALTY=2.747724
PRIMER_LEFT_2_SEQUENCE=CAAACCTATTGGAGGCAACATT
PRIMER_RIGHT_2_SEQUENCE=GCAAAACTAGACATGGATACTTGC
PRIMER_LEFT_2=604,22
PRIMER_RIGHT_2=877,24
PRIMER_LEFT_2_TM=60.237
PRIMER_RIGHT_2_TM=59.252
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=3.0097
PRIMER_LEFT_3_PENALTY=0.261998
PRIMER_RIGHT_3_PENALTY=2.747724
PRIMER_LEFT_3_SEQUENCE=TAGGCGAAGGAGTTTCAGATGT
PRIMER_RIGHT_3_SEQUENCE=GCAAAACTAGACATGGATACTTGC
PRIMER_LEFT_3=741,22
PRIMER_RIGHT_3=877,24
PRIMER_LEFT_3_TM=60.262
PRIMER_RIGHT_3_TM=59.252
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=137
PRIMER_PAIR_PENALTY_4=3.0128
PRIMER_LEFT_4_PENALTY=0.265053
PRIMER_RIGHT_4_PENALTY=2.747724
PRIMER_LEFT_4_SEQUENCE=GAAGAGGAGAAGAGCGAATCTG
PRIMER_RIGHT_4_SEQUENCE=GCAAAACTAGACATGGATACTTGC
PRIMER_LEFT_4=666,22
PRIMER_RIGHT_4=877,24
PRIMER_LEFT_4_TM=59.735
PRIMER_RIGHT_4_TM=59.252
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_00857 Ps_contig_mira-and-tgicl-ass_franssen_8208
SEQUENCE=AACAATCCCGGCTAATTTCTCAACACTCAATGTTTCACTTCGAAGCAGCGGCTCCACGCTGTCGGCGCCGCCGTCGAAATCAGCTCCTCATAGTTTTCACGGCGCGCTTGGACTGGCACACCATCCTTACGAAGAAGCTTCATACCAACACGCGCCTCTTCTAGGGTTTCATCCTCATCAACACCAACAACATCTCATGAACGCCGATCAAATCGGTGAAGCGCTTTCTAATGAGAATTACATGAGAAAACGCTATAGAGAAGATCTTTTCAAAGACGAAAACAACAACAACGAAAGCGGTGGTGATGGTTCTTCTTCTCCAAAAGCTTACAAAACAAATCACCAGCAAGATAGTATTGGTGATGGTGAAGGTGGAGGAGGAGGAGGGGGAGGAGGTGGAGTCTCTTCATCCTCTTCTCCCGGTAATTCTATTTCTGGACTTCTTAGACCGTCGAATATGTTACCGGCAACAGCCATGTGGGCCACCGCGGGTAGTGGAAGTACAATATGGATGGTACCAGTGAGTGGTGGTGGCGGTGGTGGAGGTGCTGCAACCACGAATTCAGAGACACAAACACAAAACATGTGGCCTTTTAGTCCTCATACTCAGTCTCAAAATCAAGCAAACGCTACTGCAACACCGTCTCAGTTACATTTCATGCCAAGGTTCAATCTTCCTCCCGGTGGTGGTGGTGGCGGTGTTGAATTCCAAGGTGGACGCGGTGGTTTACAACTGGGTTCAGTGTATCAGCCGTCACAGCATCTTGGATTAGCAGTGTCTGATTCAAACTTGGGTATGTTGGCTGCTCTTAATGCTTACACAAGAGCTGGTCATTTCAATGTTAACAACTCTTCCTCCGATCATCAACATCATCACCATCATTCAATGTCATTGGATTCTCATCCTCAACCTAATGATGAAAGCGGAGAAGATGCTCCTAATAATTCTCAATAACAATCCATGATTCCAAGTTGAATTCTCATCAGATTTGAGTTTTTCTCTTACATGTTCTTTTTTGGTAGGTACTTATTGTGGTATTGTGTGTTATTTACGGTTTGATTTTAGTCCAAATTAAGGGGCTTTGCAGACAGAGAGAGTTTGTATAGGAGGAAATTTCTCTTAATATTTTATGTATTTATTTCATTTTCTAGCTCTGTAATTCATGAAAAACAATAGGATTTTCAAACAAAATTTTGAATTGTTTTGACTATATATATAATTGGAAAATCTACTATTCTTT
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1161
PRIMER_LEFT_PENALTY=0.095396
PRIMER_RIGHT_PENALTY=0.020747
PRIMER_LEFT_SEQUENCE=GCCTCTTCTAGGGTTTCATCCT
PRIMER_RIGHT_SEQUENCE=GAGAAGAAGAACCATCACCACC
PRIMER_LEFT=153,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.979
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.1161
PRIMER_LEFT_1_PENALTY=0.095396
PRIMER_RIGHT_1_PENALTY=0.020747
PRIMER_LEFT_1_SEQUENCE=GCCTCTTCTAGGGTTTCATCCT
PRIMER_RIGHT_1_SEQUENCE=GGAGAAGAAGAACCATCACCAC
PRIMER_LEFT_1=153,22
PRIMER_RIGHT_1=320,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=59.979
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=168
PRIMER_PAIR_PENALTY_2=0.1844
PRIMER_LEFT_2_PENALTY=0.163658
PRIMER_RIGHT_2_PENALTY=0.020747
PRIMER_LEFT_2_SEQUENCE=TTTCATCCTCATCAACACCAAC
PRIMER_RIGHT_2_SEQUENCE=GAGAAGAAGAACCATCACCACC
PRIMER_LEFT_2=166,22
PRIMER_RIGHT_2=319,22
PRIMER_LEFT_2_TM=59.836
PRIMER_RIGHT_2_TM=59.979
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=0.1844
PRIMER_LEFT_3_PENALTY=0.163658
PRIMER_RIGHT_3_PENALTY=0.020747
PRIMER_LEFT_3_SEQUENCE=GTTTCATCCTCATCAACACCAA
PRIMER_RIGHT_3_SEQUENCE=GAGAAGAAGAACCATCACCACC
PRIMER_LEFT_3=165,22
PRIMER_RIGHT_3=319,22
PRIMER_LEFT_3_TM=59.836
PRIMER_RIGHT_3_TM=59.979
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=0.1844
PRIMER_LEFT_4_PENALTY=0.163658
PRIMER_RIGHT_4_PENALTY=0.020747
PRIMER_LEFT_4_SEQUENCE=TTTCATCCTCATCAACACCAAC
PRIMER_RIGHT_4_SEQUENCE=GGAGAAGAAGAACCATCACCAC
PRIMER_LEFT_4=166,22
PRIMER_RIGHT_4=320,22
PRIMER_LEFT_4_TM=59.836
PRIMER_RIGHT_4_TM=59.979
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_00858 Ps_contig_mira-and-tgicl-ass_franssen_8208
SEQUENCE=AACAATCCCGGCTAATTTCTCAACACTCAATGTTTCACTTCGAAGCAGCGGCTCCACGCTGTCGGCGCCGCCGTCGAAATCAGCTCCTCATAGTTTTCACGGCGCGCTTGGACTGGCACACCATCCTTACGAAGAAGCTTCATACCAACACGCGCCTCTTCTAGGGTTTCATCCTCATCAACACCAACAACATCTCATGAACGCCGATCAAATCGGTGAAGCGCTTTCTAATGAGAATTACATGAGAAAACGCTATAGAGAAGATCTTTTCAAAGACGAAAACAACAACAACGAAAGCGGTGGTGATGGTTCTTCTTCTCCAAAAGCTTACAAAACAAATCACCAGCAAGATAGTATTGGTGATGGTGAAGGTGGAGGAGGAGGAGGGGGAGGAGGTGGAGTCTCTTCATCCTCTTCTCCCGGTAATTCTATTTCTGGACTTCTTAGACCGTCGAATATGTTACCGGCAACAGCCATGTGGGCCACCGCGGGTAGTGGAAGTACAATATGGATGGTACCAGTGAGTGGTGGTGGCGGTGGTGGAGGTGCTGCAACCACGAATTCAGAGACACAAACACAAAACATGTGGCCTTTTAGTCCTCATACTCAGTCTCAAAATCAAGCAAACGCTACTGCAACACCGTCTCAGTTACATTTCATGCCAAGGTTCAATCTTCCTCCCGGTGGTGGTGGTGGCGGTGTTGAATTCCAAGGTGGACGCGGTGGTTTACAACTGGGTTCAGTGTATCAGCCGTCACAGCATCTTGGATTAGCAGTGTCTGATTCAAACTTGGGTATGTTGGCTGCTCTTAATGCTTACACAAGAGCTGGTCATTTCAATGTTAACAACTCTTCCTCCGATCATCAACATCATCACCATCATTCAATGTCATTGGATTCTCATCCTCAACCTAATGATGAAAGCGGAGAAGATGCTCCTAATAATTCTCAATAACAATCCATGATTCCAAGTTGAATTCTCATCAGATTTGAGTTTTTCTCTTACATGTTCTTTTTTGGTAGGTACTTATTGTGGTATTGTGTGTTATTTACGGTTTGATTTTAGTCCAAATTAAGGGGCTTTGCAGACAGAGAGAGTTTGTATAGGAGGAAATTTCTCTTAATATTTTATGTATTTATTTCATTTTCTAGCTCTGTAATTCATGAAAAACAATAGGATTTTCAAACAAAATTTTGAATTGTTTTGACTATATATATAATTGGAAAATCTACTATTCTTT
TARGET=374,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2865
PRIMER_LEFT_PENALTY=0.020747
PRIMER_RIGHT_PENALTY=0.265715
PRIMER_LEFT_SEQUENCE=GGTGGTGATGGTTCTTCTTCTC
PRIMER_RIGHT_SEQUENCE=CCACTCACTGGTACCATCCATA
PRIMER_LEFT=298,22
PRIMER_RIGHT=527,22
PRIMER_LEFT_TM=59.979
PRIMER_RIGHT_TM=59.734
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.2865
PRIMER_LEFT_1_PENALTY=0.020747
PRIMER_RIGHT_1_PENALTY=0.265715
PRIMER_LEFT_1_SEQUENCE=GTGGTGATGGTTCTTCTTCTCC
PRIMER_RIGHT_1_SEQUENCE=CCACTCACTGGTACCATCCATA
PRIMER_LEFT_1=299,22
PRIMER_RIGHT_1=527,22
PRIMER_LEFT_1_TM=59.979
PRIMER_RIGHT_1_TM=59.734
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=229
PRIMER_PAIR_PENALTY_2=0.3820
PRIMER_LEFT_2_PENALTY=0.020747
PRIMER_RIGHT_2_PENALTY=0.361206
PRIMER_LEFT_2_SEQUENCE=GGTGGTGATGGTTCTTCTTCTC
PRIMER_RIGHT_2_SEQUENCE=TTGTGTCTCTGAATTCGTGGTT
PRIMER_LEFT_2=298,22
PRIMER_RIGHT_2=573,22
PRIMER_LEFT_2_TM=59.979
PRIMER_RIGHT_2_TM=59.639
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.3820
PRIMER_LEFT_3_PENALTY=0.020747
PRIMER_RIGHT_3_PENALTY=0.361206
PRIMER_LEFT_3_SEQUENCE=GGTGGTGATGGTTCTTCTTCTC
PRIMER_RIGHT_3_SEQUENCE=TTTGTGTCTCTGAATTCGTGGT
PRIMER_LEFT_3=298,22
PRIMER_RIGHT_3=574,22
PRIMER_LEFT_3_TM=59.979
PRIMER_RIGHT_3_TM=59.639
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.3820
PRIMER_LEFT_4_PENALTY=0.020747
PRIMER_RIGHT_4_PENALTY=0.361206
PRIMER_LEFT_4_SEQUENCE=GTGGTGATGGTTCTTCTTCTCC
PRIMER_RIGHT_4_SEQUENCE=TTGTGTCTCTGAATTCGTGGTT
PRIMER_LEFT_4=299,22
PRIMER_RIGHT_4=573,22
PRIMER_LEFT_4_TM=59.979
PRIMER_RIGHT_4_TM=59.639
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_00859 Ps_contig_mira-and-tgicl-ass_franssen_8208
SEQUENCE=AACAATCCCGGCTAATTTCTCAACACTCAATGTTTCACTTCGAAGCAGCGGCTCCACGCTGTCGGCGCCGCCGTCGAAATCAGCTCCTCATAGTTTTCACGGCGCGCTTGGACTGGCACACCATCCTTACGAAGAAGCTTCATACCAACACGCGCCTCTTCTAGGGTTTCATCCTCATCAACACCAACAACATCTCATGAACGCCGATCAAATCGGTGAAGCGCTTTCTAATGAGAATTACATGAGAAAACGCTATAGAGAAGATCTTTTCAAAGACGAAAACAACAACAACGAAAGCGGTGGTGATGGTTCTTCTTCTCCAAAAGCTTACAAAACAAATCACCAGCAAGATAGTATTGGTGATGGTGAAGGTGGAGGAGGAGGAGGGGGAGGAGGTGGAGTCTCTTCATCCTCTTCTCCCGGTAATTCTATTTCTGGACTTCTTAGACCGTCGAATATGTTACCGGCAACAGCCATGTGGGCCACCGCGGGTAGTGGAAGTACAATATGGATGGTACCAGTGAGTGGTGGTGGCGGTGGTGGAGGTGCTGCAACCACGAATTCAGAGACACAAACACAAAACATGTGGCCTTTTAGTCCTCATACTCAGTCTCAAAATCAAGCAAACGCTACTGCAACACCGTCTCAGTTACATTTCATGCCAAGGTTCAATCTTCCTCCCGGTGGTGGTGGTGGCGGTGTTGAATTCCAAGGTGGACGCGGTGGTTTACAACTGGGTTCAGTGTATCAGCCGTCACAGCATCTTGGATTAGCAGTGTCTGATTCAAACTTGGGTATGTTGGCTGCTCTTAATGCTTACACAAGAGCTGGTCATTTCAATGTTAACAACTCTTCCTCCGATCATCAACATCATCACCATCATTCAATGTCATTGGATTCTCATCCTCAACCTAATGATGAAAGCGGAGAAGATGCTCCTAATAATTCTCAATAACAATCCATGATTCCAAGTTGAATTCTCATCAGATTTGAGTTTTTCTCTTACATGTTCTTTTTTGGTAGGTACTTATTGTGGTATTGTGTGTTATTTACGGTTTGATTTTAGTCCAAATTAAGGGGCTTTGCAGACAGAGAGAGTTTGTATAGGAGGAAATTTCTCTTAATATTTTATGTATTTATTTCATTTTCTAGCTCTGTAATTCATGAAAAACAATAGGATTTTCAAACAAAATTTTGAATTGTTTTGACTATATATATAATTGGAAAATCTACTATTCTTT
TARGET=683,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0445
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.040897
PRIMER_LEFT_SEQUENCE=TCAGTTACATTTCATGCCAAGG
PRIMER_RIGHT_SEQUENCE=TAAGCATTAAGAGCAGCCAACA
PRIMER_LEFT=645,22
PRIMER_RIGHT=818,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.041
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=0.0445
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.040897
PRIMER_LEFT_1_SEQUENCE=TCAGTTACATTTCATGCCAAGG
PRIMER_RIGHT_1_SEQUENCE=TGTAAGCATTAAGAGCAGCCAA
PRIMER_LEFT_1=645,22
PRIMER_RIGHT_1=820,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=60.041
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=0.0610
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.057411
PRIMER_LEFT_2_SEQUENCE=TCAGTTACATTTCATGCCAAGG
PRIMER_RIGHT_2_SEQUENCE=TGACCAGCTCTTGTGTAAGCAT
PRIMER_LEFT_2=645,22
PRIMER_RIGHT_2=833,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.0610
PRIMER_LEFT_3_PENALTY=0.003589
PRIMER_RIGHT_3_PENALTY=0.057411
PRIMER_LEFT_3_SEQUENCE=TCAGTTACATTTCATGCCAAGG
PRIMER_RIGHT_3_SEQUENCE=ATGACCAGCTCTTGTGTAAGCA
PRIMER_LEFT_3=645,22
PRIMER_RIGHT_3=834,22
PRIMER_LEFT_3_TM=59.996
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=190
PRIMER_PAIR_PENALTY_4=0.0743
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.070748
PRIMER_LEFT_4_SEQUENCE=TCAGTTACATTTCATGCCAAGG
PRIMER_RIGHT_4_SEQUENCE=CACTGAACCCAGTTGTAAACCA
PRIMER_LEFT_4=645,22
PRIMER_RIGHT_4=744,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=59.929
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=100
=
PRIMER_SEQUENCE_ID=SSR_00860 Ps_contig_mira-and-tgicl-ass_franssen_8208
SEQUENCE=AACAATCCCGGCTAATTTCTCAACACTCAATGTTTCACTTCGAAGCAGCGGCTCCACGCTGTCGGCGCCGCCGTCGAAATCAGCTCCTCATAGTTTTCACGGCGCGCTTGGACTGGCACACCATCCTTACGAAGAAGCTTCATACCAACACGCGCCTCTTCTAGGGTTTCATCCTCATCAACACCAACAACATCTCATGAACGCCGATCAAATCGGTGAAGCGCTTTCTAATGAGAATTACATGAGAAAACGCTATAGAGAAGATCTTTTCAAAGACGAAAACAACAACAACGAAAGCGGTGGTGATGGTTCTTCTTCTCCAAAAGCTTACAAAACAAATCACCAGCAAGATAGTATTGGTGATGGTGAAGGTGGAGGAGGAGGAGGGGGAGGAGGTGGAGTCTCTTCATCCTCTTCTCCCGGTAATTCTATTTCTGGACTTCTTAGACCGTCGAATATGTTACCGGCAACAGCCATGTGGGCCACCGCGGGTAGTGGAAGTACAATATGGATGGTACCAGTGAGTGGTGGTGGCGGTGGTGGAGGTGCTGCAACCACGAATTCAGAGACACAAACACAAAACATGTGGCCTTTTAGTCCTCATACTCAGTCTCAAAATCAAGCAAACGCTACTGCAACACCGTCTCAGTTACATTTCATGCCAAGGTTCAATCTTCCTCCCGGTGGTGGTGGTGGCGGTGTTGAATTCCAAGGTGGACGCGGTGGTTTACAACTGGGTTCAGTGTATCAGCCGTCACAGCATCTTGGATTAGCAGTGTCTGATTCAAACTTGGGTATGTTGGCTGCTCTTAATGCTTACACAAGAGCTGGTCATTTCAATGTTAACAACTCTTCCTCCGATCATCAACATCATCACCATCATTCAATGTCATTGGATTCTCATCCTCAACCTAATGATGAAAGCGGAGAAGATGCTCCTAATAATTCTCAATAACAATCCATGATTCCAAGTTGAATTCTCATCAGATTTGAGTTTTTCTCTTACATGTTCTTTTTTGGTAGGTACTTATTGTGGTATTGTGTGTTATTTACGGTTTGATTTTAGTCCAAATTAAGGGGCTTTGCAGACAGAGAGAGTTTGTATAGGAGGAAATTTCTCTTAATATTTTATGTATTTATTTCATTTTCTAGCTCTGTAATTCATGAAAAACAATAGGATTTTCAAACAAAATTTTGAATTGTTTTGACTATATATATAATTGGAAAATCTACTATTCTTT
TARGET=1210,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00861 Ps_contig_mira-and-tgicl-ass_franssen_8223
SEQUENCE=AAAGGTTTAGATAATTGGAGAAAGTGAAGAGAGAAGAGAAGAGAAACAGCTTCTTAGGTTCTTGTCATCATCAGAGACCAACACAAACACACAACAAAGGTTAATGGTTAGGTCAAGTCATGACCATGTTTCTCTTTCTCTTGGACTCAACTTGGCAAACAAGCAAGGTGATAAGAACAAGATGAAATCAAACACTTCTTTGAGTGAATGGTCACAAGGTGCATCAGCATCAACATCAAAGTAAATTTTGATGCCTCAGATGTTGATACTTCACTTCATCTATAAATCAGTARTTAAWTATTTTTTTACCTATGTTNTTATTTGTATTCTTACAATACAATATATATGTGTTTAGTTAAGTT
TARGET=30,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00862 Ps_contig_mira-and-tgicl-ass_franssen_8233
SEQUENCE=CCCGGATCCGACCGGTTCGGTTCAAGTTTCGACCGGTTTTAAAATTTGGGAAGTTGGTTTTCACGGTCACATTCCACGGGTCATTCTCTGGTTCAAGAAGGGGAGAATCACGAGCGGTTCACGTTGAGATTACCGGAGGAGGTGAGGAGCCGGTTGGTGGGTTCGACTCCCAGCCACGTGGTGAGTTGTGGTGTTACTTTTACACGAGAAGGTAGTGGGAGGAGGGGTTTCAGAACGAGGAGTGTGGGAAGTAGTGCTCCTGGGAGGAATAATGAGTGGTTCAACCGGCCGGACCGGAGGGGATTTTCTTGGACGCCACCGTTTATGGGTCGAACCGGTTCAAGTTGGTTGAAGAATAAGTCACCGGGGAAACTGGATGATGTGGGAGAACGGTCTTCTGAACGGTTGTTTGCAGGTAATCAGGTTTAGTGGAATAGATTTTATTGRATAAAAATGAAAATTTAGTTTTTTTTTAATTTACCTTTTATTGTTTTTATTTATCGTTAAAAGTTGGAAATACACATGAATCTGGTGAAGAAAGGTTTGGTGTTCATAGAAATAGGATGCGTTTATTTATTTAATTTAAATTTACTTTTCAGAATTCGAAGATTGTTTATGAAGTGGATAGAATTTTTTCTTGAATGTGGTGTTACAATTTTAAAAAAATTAAATTTAATGACGTTTAATTTATAC
TARGET=569,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00863 Ps_contig_mira-and-tgicl-ass_franssen_8238
SEQUENCE=GATCTTTCTCCCGTGTTCTCTCTCTCGCCTCTCAAACATCACGCTAAAAAACTTCGCACACTTCCGCCGCGCCTAACCTTCGCCGATGATGATGGAACACATGAACACCAGCAACCTCACCTACTTCATCTCTAAACGAATCCAACGACACCTAATCTCCACTCTGACGCGAACCGAATCTCAAGTCGAACGAGACCTAGCAACCGACAGGAAAATGGAAAGAAAGGAAGGCAARGRGAATCGAAGACATTACTCTGAATCGCAATCCAAATTCAGGGCTTGGATTTGAATTTGTTTCCGTTGTGCATCGATCTCCATTTGCTGTTTAGATTTGTAGTGGTTGATGTGGAGTTGTTACGATGTATTGAATCGCAGGGATTGAAGGAATCGCGGGGAAAAAGGATTCGAAGGTTTAGCAGGTTGCGTTCTTTCAGAAAGGTTAAAGAGGTTGATGAGGAGCGGCGGTGGTTGAGGAGAGGTTGATGAAGCGCGGCGGTGGTTGAAAGGATGTAGACGGTGGCGCGACGACAAGGAAAGGATGTTGATGGTGGCACGACGGAGGGAGAAAGGATGTGGATTGTGGTGTATTGAATCACAAGATTAAGGTAATTTATGGTGGTGGTTATGGAGGATTGAAGAAAAAGTTTGTTATCAGTGGAAAGAAAATGGAAGAGTGAGGAAGAAGAATTATGGAAAAAGAATTGAAGGGGGCCAAAATTCGTTGCTAATCAAGGTATTTATAGAATTTGAAGCTTACGTTTCTGGATATTTCAATTTTGTTTTGCCTTTGCTGCATTTTGCTTGCGTTTCATTTCTAACTTGAATGCGTTTTCACTTGCCACTGCGTTTTGGTTGCAACCTTTGTATTGCTATGTTGTCGCTGCTTTTCTGTCTTTTTGGCCTTGGCGGTTGCCGTTTTGGATTT
TARGET=17,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00864 Ps_contig_mira-and-tgicl-ass_franssen_8246
SEQUENCE=CTACCAACTGCAAAAAAAAACAACAAAAAAAAATTAWGTTGAAATTTCGTCGTTTTTGGTTTTCATTTCTCGAAGAAAAAAATACGATTGTGTTTTTCTTCATCTGTTTCGTTTCGATTCGATTCGATTCTAGCTGTTTTTATTTTTTAGATTTTTGATTTTGTTTATTGTTATAATTGGATTCTTCCTCGCAAAG
TARGET=113,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00865 Ps_contig_mira-and-tgicl-ass_franssen_8251
SEQUENCE=CGTCACTGAAAATTAGAGTCTCTTCTATTAGATGGAACATACTGCTGCAGTGAAACTTGAGTCTCCTGCACTGAAATTTGAGCCTCCTCTGCTGCTGTTGGTTCTGCCATATTATTATCACCGAGAAGTTCATTCCAATCTGGATGAAGAGAATCATCATCAACTTGCATTAACTGATCAACCCACTCCTTGAACTCCGTTGTTTTGGCATTTCCACCCAAGACGTTAGAAGCACTGTACTCCACATGATCATTCTGGATGGGAACATTATCAGGAAACGGAAGCATATCTTCAAAGAGATCTGTTCCCCAGGAAATAGCATCACTTTCTTGAGGGAATGGCATAGAATGCATTTCTGAAGCAGGGGGGAAAGGGATGTTATCAAGGAGCTCAGCAGGGTTTGAAAACATGTGTCCAGCACTTCTGTTGTTGGAATTAATCGATGCAGTCCTCATCGAGGATGAGGGATAAGATGAAGACATAGCTCCCACAAAAGGCTTCCAAGTGAATCAATCAACTTTGTATGACCTTGACATGGAAGAATGAAGAAGAAGAAGAGAAGGTGCGGCMAAAAGTTTAATTAATTAATAATATC
TARGET=545,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00866 Ps_contig_mira-and-tgicl-ass_franssen_8251
SEQUENCE=CGTCACTGAAAATTAGAGTCTCTTCTATTAGATGGAACATACTGCTGCAGTGAAACTTGAGTCTCCTGCACTGAAATTTGAGCCTCCTCTGCTGCTGTTGGTTCTGCCATATTATTATCACCGAGAAGTTCATTCCAATCTGGATGAAGAGAATCATCATCAACTTGCATTAACTGATCAACCCACTCCTTGAACTCCGTTGTTTTGGCATTTCCACCCAAGACGTTAGAAGCACTGTACTCCACATGATCATTCTGGATGGGAACATTATCAGGAAACGGAAGCATATCTTCAAAGAGATCTGTTCCCCAGGAAATAGCATCACTTTCTTGAGGGAATGGCATAGAATGCATTTCTGAAGCAGGGGGGAAAGGGATGTTATCAAGGAGCTCAGCAGGGTTTGAAAACATGTGTCCAGCACTTCTGTTGTTGGAATTAATCGATGCAGTCCTCATCGAGGATGAGGGATAAGATGAAGACATAGCTCCCACAAAAGGCTTCCAAGTGAATCAATCAACTTTGTATGACCTTGACATGGAAGAATGAAGAAGAAGAAGAGAAGGTGCGGCMAAAAGTTTAATTAATTAATAATATC
TARGET=577,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00867 Ps_contig_mira-and-tgicl-ass_franssen_8256
SEQUENCE=CAAGTTCAAATTCAGAACAGACGAAGATTCCCCAAGGGCAATAATTGTAGTCGCATTTATCATGAGGCCACCAATCAATAGTAGCACAAATGAAATTATCATCATTTTCAGCAATTGCTTGAAATCCATCTACTATAATCAAACCGTTTTCAACTTCTTGAGTTAACACCGAATGAAGAGAAGCCGGAAAGAGAAACAAGATGAGATAGAATCCCATTTGTTAGCAGCAGATCAATCCAACACCAACTCAACTTATCCAGGTTAATTTTCTCAATCTTGTTTGGATCGACCAATAATGATGTTGTTGTTGTTGTTAAGTTAGTGAG
TARGET=300,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00868 Ps_contig_mira-and-tgicl-ass_franssen_8273
SEQUENCE=GATTGATTGATGGACGCACAAAACAAACTGCACCAGACAGGTGGGGTCCACACTGCCATTTCCTTTCATTTCTCTTCTTCTCCTTCTTCTTCACATTCCATCATCACTATTCTCACCTAACCATTTATTACTCTCTTCAAATTCAGACACCCTTTATCGTTTCCTGTCCAAATTGTTCAAAAAAAATCTGATTTTGGTGGGTCGATGTCTACGCTTTCTAAAGATGATAACTTGGCTCTTTCTTCTGAGGATTCTTCTAGCCCGGATGAGTCTGAGCTTGAATTGGGTCTTGGGTTGAGTCTTTCTCATCCTGCAAAGTCACACCAGTATCATCATCATCATGGTAACTATGCTAGGATTTTCACTGCTAAGGATTTTTCTTCTTCACCTTCCTCTGTTGGAACTAAGAGACCTGCTGATTCTCTTGATGCTACCAACCGTCCCAGTCAAGTAGTTGGATGGCCTCCCCTTCGAACATACCGAGTGAATAGCTTTAATGCCAACGTGAAATCAACTGAAGCATTTGACTCTGTTGCTGAGAAGAACAAGAACAATAATACTGGGGCAAGGAAGAGTACTGACAACGGAAAGGATGATAATAACATTAATGTCAAAGATAAAAGAAACCTCAGGAGTTCCCCCTTTGTCAAAGTAAATATGGATGGAATACCAATTGGAAGGAAGGTTGATTTGAGCGCTCACAGTTCCTACGAAACATTGGCAGAGACATTGGAGGACATGTTTGACGAATCAACCACAGGCGTCACTTGCAAAGGATCAGATGGCGAGGATCACAACATGACTATCAGAGGAGAAAAGCTTTCGATACTGTTGGATGGATCTTCCAAGTTTGTACTCACTTATGAAGACAAGGAAGGAGATTGGATGCTTGTTGGAGATGTTCCTTGGGGAATGTTCCTTAGTTCCGTGAAGAGGCTAAGAATCATGAGGACATCCGAGGCTAATGGACTTGCACCAAGATTGGAAGAAAAGAGCAGCAGACCGAAAAGCAAGCCTAAATAGATATTGTTTTCATGGCTAAGGATAAGAACTTACTTATCTACCTTATATGTAACACAAATAGAAGGAGGAAAAACAAAAACAAAAACAAAAAGTATGAAGTACAGCAAGTAAATGGTATTTTGAGTTTACAAATAG
TARGET=329,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0663
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=0.059316
PRIMER_LEFT_SEQUENCE=TCTTTCTCATCCTGCAAAGTCA
PRIMER_RIGHT_SEQUENCE=AGCAACAGAGTCAAATGCTTCA
PRIMER_LEFT=299,22
PRIMER_RIGHT=536,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=60.059
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.0663
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=0.059316
PRIMER_LEFT_1_SEQUENCE=TCTTTCTCATCCTGCAAAGTCA
PRIMER_RIGHT_1_SEQUENCE=GCAACAGAGTCAAATGCTTCAG
PRIMER_LEFT_1=299,22
PRIMER_RIGHT_1=535,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=60.059
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=0.0663
PRIMER_LEFT_2_PENALTY=0.006945
PRIMER_RIGHT_2_PENALTY=0.059316
PRIMER_LEFT_2_SEQUENCE=TGAGTCTTTCTCATCCTGCAAA
PRIMER_RIGHT_2_SEQUENCE=AGCAACAGAGTCAAATGCTTCA
PRIMER_LEFT_2=295,22
PRIMER_RIGHT_2=536,22
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=60.059
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.0663
PRIMER_LEFT_3_PENALTY=0.006945
PRIMER_RIGHT_3_PENALTY=0.059316
PRIMER_LEFT_3_SEQUENCE=TGAGTCTTTCTCATCCTGCAAA
PRIMER_RIGHT_3_SEQUENCE=GCAACAGAGTCAAATGCTTCAG
PRIMER_LEFT_3=295,22
PRIMER_RIGHT_3=535,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=60.059
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=0.0663
PRIMER_LEFT_4_PENALTY=0.006945
PRIMER_RIGHT_4_PENALTY=0.059316
PRIMER_LEFT_4_SEQUENCE=TGAGTCTTTCTCATCCTGCAAA
PRIMER_RIGHT_4_SEQUENCE=CAGCAACAGAGTCAAATGCTTC
PRIMER_LEFT_4=295,22
PRIMER_RIGHT_4=537,22
PRIMER_LEFT_4_TM=59.993
PRIMER_RIGHT_4_TM=60.059
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_00869 Ps_contig_mira-and-tgicl-ass_franssen_8283
SEQUENCE=CTCCCATTGACGTTCCACCCATTGTTCAWTCCTTCTTTGATCACCACAGAGCAGTTAACCATGACGGTCACACCATGTCTTTGTTATCCATCCAAGTCACTGAATTATTGGATGGTGTTTTCATAGTTGTTCCATGAATCACTCTATTGGYGATGGAACTGCTTATTGGAATTTCTTTAACACATTTTCTGAGATATTTCAAAATGATGGCAGTGTTCATGTTCCAATTTCACACCAACCCATCCACAATAAGTTGTTTCCAGAAGGTTATGGCCCAATTATCAACCTTCCTTTCAAACATCACGACGAGTTTATTCAAAGATATAAAGCACCGATTTTGAGAGAGAGAATCTTCCATTTTTCAGCAGAATCTATAGCAAAAATCAAAGCAAAGGCTAACGAAGAATCTAACACAAACAAAATCTCTTCGTTCCAATCTTTATCAGCACTAGTTTGGAGATCTATAACGCGTGTGCGTCAACTACAACATGATACAGAAAACAACTTGCAACTTGGCTATAAACAACCGAACGAGAACGGACCNCCTATTCCTAAGGATTTATTTTAGGAAATTCCGTTAATGCAGTGAAAG
TARGET=340,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00870 Ps_contig_mira-and-tgicl-ass_franssen_8308
SEQUENCE=CACAACGAGTATATGTTTTACTGTTGTAAAGGTTCAATAGATCTTACAACAAAATGACGCGTCTTACCACCTTCATTGTTTAGGGGACCATACCAAAATCTGGAGATGATTTTATTCTAACAGAATGATCCGTTGTATTTGACCAGCTCACAAATTTTGTACACAATTCCAAAATAACATATTTCCTCTATAAATAAGTAGGTTCCAATCATTGAGAAGGATATATCGTTACCCTGCCGAAATCCCACCTCAACCTGTACTCGAGGTAAAGAATAGTTGTCGGCGTCTCGCCGGAGTCGCGAAGCTTCCGGTCGGGGATCTTTTGCCTCTTTCTCCAACCTTTTCAAGGCGTCGAAACTCCATGTGTTAGCCGCTTGCATGTTTGATTTGATGATTGTTTATTTATGCATGACCGTTTGTATTAGAACTAGACTATTGTAGATTTAAAGATAATCTTGCTGCGACCCGTGAAATTCGATATTAGATCTGTTTTTTTTCGGTTCGATTTTGCTTGTGCTTTTTGTTTGGGTGTGATTATTTTCCTCTTTGTTGAAAACCAAGAACTAGTGTTAACGAAGAGAGAGAGGAGTCGTTTCGG
TARGET=577,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00871 Ps_contig_mira-and-tgicl-ass_franssen_8309
SEQUENCE=TCGATTATTAGANCTTTGGTTTTTTTTCGGTTCGTATTTTGCTTGTGTCTTTTTKTTTGGGTGTGATATTTTCCTCTTTGTTGAAACCAAGAACTAGTGTTAACGAAGAGAGAGAGGAGTCGTCGTTTCGGTTTTTTTGAGTTAGTGAGGATGAACACCGTGAGATGTTCATCCTTGGTTCTATGAGTTGAAGACATTTTCAGTTTCCTCTTGTTTTTTTAGAAAGGTTTTTAGTTTGATGTTAGAAGAAAGAAGGTGAGCATGTATGCATGACCTTTAGC
TARGET=107,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00872 Ps_contig_mira-and-tgicl-ass_franssen_8321
SEQUENCE=CCTTTTCTCCTAAGCTTTTTCTCCCTTTCTTTTGTTCTTTTCCTCTCCCTGCGCTTTCTTCTTCTTCACGTTTTTCCTTTTCCTCCTCTTCAAGAAGTTTTGTCTGCTTTTCTAAATGTAACGATCT
TARGET=56,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.0431
PRIMER_LEFT_PENALTY=1.776417
PRIMER_RIGHT_PENALTY=2.266650
PRIMER_LEFT_SEQUENCE=TTTCTCCTAAGCTTTTTCTCCC
PRIMER_RIGHT_SEQUENCE=GACAAAACTTCTTGAAGAGGAGGA
PRIMER_LEFT=3,22
PRIMER_RIGHT=103,24
PRIMER_LEFT_TM=58.224
PRIMER_RIGHT_TM=60.267
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=4.2056
PRIMER_LEFT_1_PENALTY=1.776417
PRIMER_RIGHT_1_PENALTY=2.429181
PRIMER_LEFT_1_SEQUENCE=TTTCTCCTAAGCTTTTTCTCCC
PRIMER_RIGHT_1_SEQUENCE=GACAAAACTTCTTGAAGAGGAGG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=103,23
PRIMER_LEFT_1_TM=58.224
PRIMER_RIGHT_1_TM=58.571
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=4.2979
PRIMER_LEFT_2_PENALTY=1.776417
PRIMER_RIGHT_2_PENALTY=2.521503
PRIMER_LEFT_2_SEQUENCE=TTTCTCCTAAGCTTTTTCTCCC
PRIMER_RIGHT_2_SEQUENCE=AGACAAAACTTCTTGAAGAGGAGG
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=104,24
PRIMER_LEFT_2_TM=58.224
PRIMER_RIGHT_2_TM=59.478
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=102
PRIMER_PAIR_PENALTY_3=4.4987
PRIMER_LEFT_3_PENALTY=2.232077
PRIMER_RIGHT_3_PENALTY=2.266650
PRIMER_LEFT_3_SEQUENCE=CTTTTCTCCTAAGCTTTTTCTCCC
PRIMER_RIGHT_3_SEQUENCE=GACAAAACTTCTTGAAGAGGAGGA
PRIMER_LEFT_3=1,24
PRIMER_RIGHT_3=103,24
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=60.267
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=4.5061
PRIMER_LEFT_4_PENALTY=2.232077
PRIMER_RIGHT_4_PENALTY=2.274070
PRIMER_LEFT_4_SEQUENCE=CTTTTCTCCTAAGCTTTTTCTCCC
PRIMER_RIGHT_4_SEQUENCE=CAAAACTTCTTGAAGAGGAGGA
PRIMER_LEFT_4=1,24
PRIMER_RIGHT_4=101,22
PRIMER_LEFT_4_TM=60.232
PRIMER_RIGHT_4_TM=57.726
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_00873 Ps_contig_mira-and-tgicl-ass_franssen_8328
SEQUENCE=CACTGGAAAGTTTAGATATGGCCATCAACAGTAAAAAAAAATCTTTCTTTCTTTGATTTTTTCTTCCTCTAATCCAGAGTGGGTCTGGTGGGACGCTATCTGCGCAAAAGCTTCTAGACTTACTTCTGTGCTGCACCGCCACGTATGGGCACACCCGCGCTGCTCCTCTCTGGTACTATAGTGCCGCAACATGGTGCTGGCCTTTCCTTGATAACGCACATCTAGAATCACCAGCTATTTGACAAACTAAAAAACGTTTTTCCGTTTTTCCCTTACCGGGTAA
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00874 Ps_contig_mira-and-tgicl-ass_franssen_8382
SEQUENCE=GACACGCATGCTCCAAGTTTGAATTTCGAAGCCAAATCGATACTCTCATGGTTATGGCTTCTTCTTCTTCTTCTTCGCCCGAGGAGCAATGTGGAGCTGAGGTTTCCGATAATTATGAGACGACATGCTGGGGTTGTGGACTGCGCGTTATGCTTCCATCATGTGCGTCCATTTTCAAATGTGGTTGGTGTGGGGCCTTACAGATACAAAAACAAAAAGAACGTGAACGAAAAGTGCCG
TARGET=58,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9043
PRIMER_LEFT_PENALTY=0.224014
PRIMER_RIGHT_PENALTY=0.680280
PRIMER_LEFT_SEQUENCE=TTCGAAGCCAAATCGATACTCT
PRIMER_RIGHT_SEQUENCE=GCACTTTTCGTTCACGTTCTTT
PRIMER_LEFT=24,22
PRIMER_RIGHT=236,22
PRIMER_LEFT_TM=60.224
PRIMER_RIGHT_TM=60.680
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.9165
PRIMER_LEFT_1_PENALTY=0.224014
PRIMER_RIGHT_1_PENALTY=0.692533
PRIMER_LEFT_1_SEQUENCE=TTCGAAGCCAAATCGATACTCT
PRIMER_RIGHT_1_SEQUENCE=TTTTTGTATCTGTAAGGCCCCA
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=60.224
PRIMER_RIGHT_1_TM=60.693
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=188
PRIMER_PAIR_PENALTY_2=1.0282
PRIMER_LEFT_2_PENALTY=0.347935
PRIMER_RIGHT_2_PENALTY=0.680280
PRIMER_LEFT_2_SEQUENCE=GCCAAATCGATACTCTCATGGT
PRIMER_RIGHT_2_SEQUENCE=GCACTTTTCGTTCACGTTCTTT
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=236,22
PRIMER_LEFT_2_TM=60.348
PRIMER_RIGHT_2_TM=60.680
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=1.0405
PRIMER_LEFT_3_PENALTY=0.347935
PRIMER_RIGHT_3_PENALTY=0.692533
PRIMER_LEFT_3_SEQUENCE=GCCAAATCGATACTCTCATGGT
PRIMER_RIGHT_3_SEQUENCE=TTTTTGTATCTGTAAGGCCCCA
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=60.348
PRIMER_RIGHT_3_TM=60.693
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=1.1515
PRIMER_LEFT_4_PENALTY=0.471231
PRIMER_RIGHT_4_PENALTY=0.680280
PRIMER_LEFT_4_SEQUENCE=AGCCAAATCGATACTCTCATGG
PRIMER_RIGHT_4_SEQUENCE=GCACTTTTCGTTCACGTTCTTT
PRIMER_LEFT_4=29,22
PRIMER_RIGHT_4=236,22
PRIMER_LEFT_4_TM=60.471
PRIMER_RIGHT_4_TM=60.680
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_00875 Ps_contig_mira-and-tgicl-ass_franssen_8390
SEQUENCE=TTTTTCCTTGCAACAGTTTCNGTTTCACGGATACCTGGTTGAAACGCTGGAATGATTCCTCTTCCAAACACTCCCGTCCAAAACCGCCGCGTGCCATTCTAGATCATTCAAGCTCTGATGATGAGGATGTTGGTGGTAGTAGAATGGATAGGATTGTTGAGAAATTGAAGAAGTTTGGGTATGAAAGTGATGGGGTTGAGAATAAAACAGAAGAGAGTGTGATTGAAAAAGGGTCCGTGGAGGATATATTTTATGTGGAAGAAGGAATGTTGCCTAATACAAGAGGTGGGTTTTCGTCGGAATCTCCATTTGGGGTTGGAAGATTTGGAAGTGATGGTGGTGGTGGAGAAGTAAGGTTTCCTTGGGAGAAACCAGTGGTGGATAAAGAGGTTGAAGAGAGGAGGGCTTTGAGGAAAAAAAAGTAAGACTTCTATGGCGGAATTGACTCTTCCT
TARGET=335,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2032
PRIMER_LEFT_PENALTY=0.107763
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=GTTGCCTAATACAAGAGGTGGG
PRIMER_RIGHT_SEQUENCE=AAGAGTCAATTCCGCCATAGAA
PRIMER_LEFT=268,22
PRIMER_RIGHT=449,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=0.2032
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=TTGCCTAATACAAGAGGTGGGT
PRIMER_RIGHT_1_SEQUENCE=AAGAGTCAATTCCGCCATAGAA
PRIMER_LEFT_1=269,22
PRIMER_RIGHT_1=449,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=0.2032
PRIMER_LEFT_2_PENALTY=0.107763
PRIMER_RIGHT_2_PENALTY=0.095445
PRIMER_LEFT_2_SEQUENCE=TGCCTAATACAAGAGGTGGGTT
PRIMER_RIGHT_2_SEQUENCE=AAGAGTCAATTCCGCCATAGAA
PRIMER_LEFT_2=270,22
PRIMER_RIGHT_2=449,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.2470
PRIMER_LEFT_3_PENALTY=0.151533
PRIMER_RIGHT_3_PENALTY=0.095445
PRIMER_LEFT_3_SEQUENCE=GAGTGTGATTGAAAAAGGGTCC
PRIMER_RIGHT_3_SEQUENCE=AAGAGTCAATTCCGCCATAGAA
PRIMER_LEFT_3=214,22
PRIMER_RIGHT_3=449,22
PRIMER_LEFT_3_TM=59.848
PRIMER_RIGHT_3_TM=60.095
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.3166
PRIMER_LEFT_4_PENALTY=0.095629
PRIMER_RIGHT_4_PENALTY=0.220992
PRIMER_LEFT_4_SEQUENCE=CGTGCCATTCTAGATCATTCAA
PRIMER_RIGHT_4_SEQUENCE=TTTATCCACCACTGGTTTCTCC
PRIMER_LEFT_4=89,22
PRIMER_RIGHT_4=385,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=60.221
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_00876 Ps_contig_mira-and-tgicl-ass_franssen_8396
SEQUENCE=TAAGCCCGCAGTCAAACARGTTGACAGTTTTTTTTGAGATTTGAATTGAAGTCCCACTCAAGTACGTTGTCCTAAACACGTAGTAAGATAGAAAGAGAAAAGAAAACCCTCAATTCGAGTTTCAAATTTCACAATCCGATTCTCAACCTTACAGAAGAACAACAACAAGACCTTATTCAACAACATGACTAGTGTTGATCAACCTCAAGTCCAACCCTCCGACTCTCACTCCCATACCCCCTCCGATTACGCTCCATATCCTAAAATTGACCCAAACGACGTCGCACCACCACCACCACCATCTCAATCCGTCGCAACTGAATCACGCGCCACCGATGCTGCCACCACTATGCCCACCGAGTCTAATCCATATGTCTCTCCCGCGCCTGTCCCCGCACCCACACCTGCAAAGAATACTTTAGATTCCGTGAAAGATGTTCTCGGAAAATGGGGAAAGAAAGCTGCTGAAGCTACCAAGAAAGCCGAGGATCTTGCTGGGAATATGTGGCAGCACTTGAAAACGGGTCCTAGTTTTGCGGATGCAGCTGTGGGGAGGATTGCTCAGGGAACAAAAGTTCTGGCAGAAGGTGGTTATGAGAAGATCTTTAGGCAAACTTTTGAGACTGTACCCGAGGAACAACTTCTTAAAACATATGCGTGCTATCTCTCCACCTCTGCTGGACCTGTCATGGGAGTCTTGTATTTGTCATCTGCAAAGCTCGCATTTTCTAGTGATAACCCGCTCTCTTATCAAACCGGTGACCAGACTCAATGGAGCTATTATAAGGTGGTTATTCCATTGCATCAGCTGAGAGCCGTGAACCCATCCACAAGCAAAGTCAACCCATCCGAAAAATTTATACAGATTATCTCCGTCGACAACCATGAATTTTGGTTTATGGGCTTTGTGAACTATGACAGCGCTGTTAAACATATTCAAGAAGCATTGCAGTCTCGCTGATACACGCTGAACTGAAAAGCGTCATGTATTATACCAAAGATGAGGTCTGTGTGATAAACTATCCTCAATTCACAACTTGAAACATTATATCTTTATACTGTAGTTGGTAAGTCTAGTTTGTGGAGCCGTTATAGTTGAGATCGAAACACATTTCTTATTTCTGGCATTATGTGTTTTACATGTGTGAGAGTTAATTGATTTGCTGTAAGTTAAACCAATGTACTTGCGTTTCTTATATCTTATTCTATTCAATTGTACTTTTTCTTTTCTTAAAA
TARGET=285,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00877 Ps_contig_mira-and-tgicl-ass_franssen_8405
SEQUENCE=GAATAATGTACAGAAAACATCATGTTGTGAAGATTTAGTTTACCGAATAAGTTGTAAAACATGATTGAAGTACCTTATTGGTTTAACATTGTTTGAAGGAATGTAGATGGTAAGAATAGTTAGGATTCAATCATAATAAACCATTTCCATGGCCAAAAGTTTCGAATAATCCTGATCGAGCAAAATCCTTATCATGTCATCGACGATTGAKTGTTTTGGTAAGAAAACCTGAAATGCTCTAGATATATATATTCTTGATTGACTTCTCAGATTTCTTGTA
TARGET=243,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00878 Ps_contig_mira-and-tgicl-ass_franssen_8413
SEQUENCE=CCAACTCCTTCGCACCGCCCAGAAACAAACCAACAACAGACGCTCTTACAGTTTTTTTCTTTTGACAGTTTTAGAAGTTATAGGCTGCAAAAGAAGCCTCATGAAAAGCCTATTATCAGTAGTTGGAGCAACTTGCTGCCATTGAACAACAAGATCAGTAGCATTCTGTTCCAAAGTTTTCTCAATTCTGAAAACAGTTACAGTTTCAGGAACCGCTACTAATTTAACAGTATAAGATAAAATAACACCAAAACTAGCACCACCACCACCTCTTATAGCCCAAAATAGATCTTCCCCCATTGATTTTCTATTCAAAAGCCTTCCCTTCACATCAACAATTTCAGCATCAATRATATTATCARYAGAWARACCATAYTTTCTCAACATTGTACCATACCCTCCACCACTGATGTGACCACCAACACCAACAGTTGGGCACACACCAGCAGGAAAGCCATGAAGTTTGCTTCTTTTTCGTAAATTACTATTAAA
TARGET=258,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00879 Ps_contig_mira-and-tgicl-ass_franssen_8439
SEQUENCE=CGTTTCGGGGGCCCGTGAACCCCTTCACCATCAAACTCAACAAAACACAACCCAATCTCTCTCTCTCTCTTATTTTTCTTCTCTTCTCCACCTTCTTCTTTTTCTCTCTCAACCTAATTAAAATTTGACAGTGTAATCATGTAGAACAAATT
TARGET=56,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=5.6483
PRIMER_LEFT_PENALTY=2.758218
PRIMER_RIGHT_PENALTY=2.890131
PRIMER_LEFT_SEQUENCE=GTGAACCCCTTCACCATCAA
PRIMER_RIGHT_SEQUENCE=GGTTGAGAGAGAAAAAGAAGAAGG
PRIMER_LEFT=14,20
PRIMER_RIGHT=113,24
PRIMER_LEFT_TM=60.758
PRIMER_RIGHT_TM=59.110
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=5.6635
PRIMER_LEFT_1_PENALTY=2.773400
PRIMER_RIGHT_1_PENALTY=2.890131
PRIMER_LEFT_1_SEQUENCE=GTGAACCCCTTCACCATCAAAC
PRIMER_RIGHT_1_SEQUENCE=GGTTGAGAGAGAAAAAGAAGAAGG
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=113,24
PRIMER_LEFT_1_TM=62.773
PRIMER_RIGHT_1_TM=59.110
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=5.8106
PRIMER_LEFT_2_PENALTY=2.758218
PRIMER_RIGHT_2_PENALTY=3.052398
PRIMER_LEFT_2_SEQUENCE=GTGAACCCCTTCACCATCAA
PRIMER_RIGHT_2_SEQUENCE=AGGTTGAGAGAGAAAAAGAAGAAGG
PRIMER_LEFT_2=14,20
PRIMER_RIGHT_2=114,25
PRIMER_LEFT_2_TM=60.758
PRIMER_RIGHT_2_TM=59.948
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=101
PRIMER_PAIR_PENALTY_3=5.8258
PRIMER_LEFT_3_PENALTY=2.773400
PRIMER_RIGHT_3_PENALTY=3.052398
PRIMER_LEFT_3_SEQUENCE=GTGAACCCCTTCACCATCAAAC
PRIMER_RIGHT_3_SEQUENCE=AGGTTGAGAGAGAAAAAGAAGAAGG
PRIMER_LEFT_3=14,22
PRIMER_RIGHT_3=114,25
PRIMER_LEFT_3_TM=62.773
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=5.9229
PRIMER_LEFT_4_PENALTY=2.758218
PRIMER_RIGHT_4_PENALTY=3.164715
PRIMER_LEFT_4_SEQUENCE=GTGAACCCCTTCACCATCAA
PRIMER_RIGHT_4_SEQUENCE=GGTTGAGAGAGAAAAAGAAGAAGGT
PRIMER_LEFT_4=14,20
PRIMER_RIGHT_4=113,25
PRIMER_LEFT_4_TM=60.758
PRIMER_RIGHT_4_TM=59.835
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=100
=
PRIMER_SEQUENCE_ID=SSR_00880 Ps_contig_mira-and-tgicl-ass_franssen_8450
SEQUENCE=CTAAAATCAAGAACCCTAAGCTATGAAAAGTGAAATTAAATGGTTGGAGCTTCAATCTGAACCCACACCTTAGGGGTTTGGTTCAGGTTACGCAGGGCTACATTGCGGGTAAAGGAATTTGGAGCAAAGGGAAAGGAAAGGAAAATCAAATGCCGACCTACGAGACCATGGCTTATCCCGAAATTTGTTGCAACAAAGAAGATGAAGTCGAAGCAAGAACACAAAAGGCTCTACTGAGGTTGGTAGAATCGAAATTACCTTCATCGTAGAGAAGCTTGGCTTCAACAAAAGCAATAACACCAAGCTCTACTGTAGTTTTAGTT
TARGET=130,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4744
PRIMER_LEFT_PENALTY=0.394672
PRIMER_RIGHT_PENALTY=0.079714
PRIMER_LEFT_SEQUENCE=CTTCAATCTGAACCCACACCTT
PRIMER_RIGHT_SEQUENCE=TGTGTTCTTGCTTCGACTTCAT
PRIMER_LEFT=49,22
PRIMER_RIGHT=222,22
PRIMER_LEFT_TM=60.395
PRIMER_RIGHT_TM=59.920
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=0.6089
PRIMER_LEFT_1_PENALTY=0.529160
PRIMER_RIGHT_1_PENALTY=0.079714
PRIMER_LEFT_1_SEQUENCE=TTCAATCTGAACCCACACCTTA
PRIMER_RIGHT_1_SEQUENCE=TGTGTTCTTGCTTCGACTTCAT
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=222,22
PRIMER_LEFT_1_TM=59.471
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.6727
PRIMER_LEFT_2_PENALTY=0.593025
PRIMER_RIGHT_2_PENALTY=0.079714
PRIMER_LEFT_2_SEQUENCE=CTTAGGGGTTTGGTTCAGGTTA
PRIMER_RIGHT_2_SEQUENCE=TGTGTTCTTGCTTCGACTTCAT
PRIMER_LEFT_2=68,22
PRIMER_RIGHT_2=222,22
PRIMER_LEFT_2_TM=59.407
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.6821
PRIMER_LEFT_3_PENALTY=0.394672
PRIMER_RIGHT_3_PENALTY=0.287386
PRIMER_LEFT_3_SEQUENCE=CTTCAATCTGAACCCACACCTT
PRIMER_RIGHT_3_SEQUENCE=CCAACCTCAGTAGAGCCTTTTG
PRIMER_LEFT_3=49,22
PRIMER_RIGHT_3=242,22
PRIMER_LEFT_3_TM=60.395
PRIMER_RIGHT_3_TM=60.287
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=0.7629
PRIMER_LEFT_4_PENALTY=0.394672
PRIMER_RIGHT_4_PENALTY=0.368190
PRIMER_LEFT_4_SEQUENCE=CTTCAATCTGAACCCACACCTT
PRIMER_RIGHT_4_SEQUENCE=TGCTTCGACTTCATCTTCTTTG
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=214,22
PRIMER_LEFT_4_TM=60.395
PRIMER_RIGHT_4_TM=59.632
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_00881 Ps_contig_mira-and-tgicl-ass_franssen_8465
SEQUENCE=GGCGATTCCATACATCGCAGAACGGATTAGTTCTAGAATTGTCTTTCCCTTAAGGTCATTATCCTGTCTGATTACGAGTATGCTGAGATTTTTGGTGAAAGTGCCCGCGGGAGTAGTAGTAGTCGGGTTTCTTCGGGGAGTGAACTTGCTGCTTCGT
TARGET=112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7623
PRIMER_LEFT_PENALTY=0.369507
PRIMER_RIGHT_PENALTY=1.392768
PRIMER_LEFT_SEQUENCE=CATACATCGCAGAACGGATTAG
PRIMER_RIGHT_SEQUENCE=AAGTTCACTCCCCGAAGAAAC
PRIMER_LEFT=8,22
PRIMER_RIGHT=146,21
PRIMER_LEFT_TM=59.630
PRIMER_RIGHT_TM=59.607
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=139
PRIMER_PAIR_PENALTY_1=2.3728
PRIMER_LEFT_1_PENALTY=0.369507
PRIMER_RIGHT_1_PENALTY=2.003322
PRIMER_LEFT_1_SEQUENCE=CATACATCGCAGAACGGATTAG
PRIMER_RIGHT_1_SEQUENCE=GAAGCAGCAAGTTCACTCCC
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=154,20
PRIMER_LEFT_1_TM=59.630
PRIMER_RIGHT_1_TM=59.997
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=2.5937
PRIMER_LEFT_2_PENALTY=0.369507
PRIMER_RIGHT_2_PENALTY=2.224191
PRIMER_LEFT_2_SEQUENCE=CATACATCGCAGAACGGATTAG
PRIMER_RIGHT_2_SEQUENCE=CAAGTTCACTCCCCGAAGAA
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=147,20
PRIMER_LEFT_2_TM=59.630
PRIMER_RIGHT_2_TM=60.224
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=2.5988
PRIMER_LEFT_3_PENALTY=0.369507
PRIMER_RIGHT_3_PENALTY=2.229246
PRIMER_LEFT_3_SEQUENCE=CATACATCGCAGAACGGATTAG
PRIMER_RIGHT_3_SEQUENCE=CAAGTTCACTCCCCGAAGAAAC
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=147,22
PRIMER_LEFT_3_TM=59.630
PRIMER_RIGHT_3_TM=62.229
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=140
PRIMER_PAIR_PENALTY_4=2.7893
PRIMER_LEFT_4_PENALTY=1.396516
PRIMER_RIGHT_4_PENALTY=1.392768
PRIMER_LEFT_4_SEQUENCE=CATACATCGCAGAACGGATTAGT
PRIMER_RIGHT_4_SEQUENCE=AAGTTCACTCCCCGAAGAAAC
PRIMER_LEFT_4=8,23
PRIMER_RIGHT_4=146,21
PRIMER_LEFT_4_TM=60.397
PRIMER_RIGHT_4_TM=59.607
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_00882 Ps_contig_mira-and-tgicl-ass_franssen_8466
SEQUENCE=GKATGCAAGAGTTKGGGGATAACACGAACTTCGTTAATACTGTGTCAACAGAAAAATCCATGGGGTGGGGTCACCTTGGAAGACGTGGCTCAGATTCCGGCAACGACGCCGATGCAGGTCCGAGCACCAGCACGGCGGAGCAACGCCGTTTTCCTTCAGCTGCGCAGCCAGAGATGATGAGAGCAGCTGAAAAAGACGATCAGTACGCCTCTTTCGTTTACGAAGCCTGTCGCGACGCCTTCCGCCACCTCTTCGGTACTAGAGTTGCGGTGGCTTATCAAAATGAGACGAAGCTTTTAGGGCAAATGCTTTACTTTGTTCTCACAACAGGCTCAGGACAACAAACACTTGGAGAGGAATACTGTGACATCACTCAGGTTGCAGGACCTTATGGACTTCCTCCAACACCGGCCAGGCGCGCTTTGTTTATTGTATACCAAACGGCGATTCCATACATCGCAGAACGGATTAGTTCTAGAATTGCTTCCCAAGGCATTATCCTGTCTGATTACGAGTATGCTGAGTATTTTTGGTGAAAGTGCCCGCGGGAGTAGTAGTAGTCGGGTTTCGTTCGGGGAGTGAACTTGCTGTCTTCGTCTG
TARGET=550,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00883 Ps_contig_mira-and-tgicl-ass_franssen_8469
SEQUENCE=CTCTCTTCCTAATCAAACCGCCAAAAACAGCAGAAGGGACATCTTTTTGAGCAATTTCAATCTTATCATCATCATCATCAGAGTCGTTTTCTTCGGGTAACTCAATTTCCTCATGGTTTGCATTAGACTTTATCCCTCCGTCCGACTGTGACTCCACTCCGGCACTTACAAACCCAACTTTTCTCTCTTTGGTCACTGACTTGTCAGCGGCTGGGGCTAATTGCCTTTCTAAAGCAGCCATTTCATCCTCAGGGATCCCAGCCTCTTTAAGTTTGTCTTTTGGCTTCATCAAGATTCACAGTTTGATCCTTCTGCATCAGATACTCTGGCAGTATAAAATAGTGTCTGGCTGTAGCTTGCAGACACACTCCGTTTAATTCGAAGCATTTCTCTAAATGTATCTTCATTTCCATGCTGAACCTCAAATTCATGCCACTTATTCCAGAAGTCGGGATCAGACCGTGGATCAGCAAACTTTGAAGCAAAAACATATATACCACGGGCACGCTCAATTTCTCCCAAACTTCTTTCCAGCTCGGCATACTTCAAACACATAGCTTTGACATCCTTATCTGGAAGACCAGATTCAATTGCTTGCTCATAGATCTCTCGTGTTTTGGGTACGCCAAATATCTCAGCTGCGCGAGAAATGTATATTTCATACATGCTCAACTTCTCATTGTTGGGCACAGCTTTCGTGGCTTGATCATAAACCTTCATAGCTCGCTTTGCTAGTCCATAATCCTCCTCCAGCTTAGCATACTGCAGATAGAGAGGCTTCACTTGATCAGCCGGGGCCGTTTCAACCGCATTCTCAAACAGCTCTCTTGCTCTTTCCAATTTTGTCCTTCCATATCTCTTCACAAATTTAGACAGATATGTAACCCAAATATCCTTAACATGTGGGTACTTAAATATCTTCACTCCTCTTTCATAAACCTTGAACGCATCTTCAAAGTATTTGTGCTCCTCCAGAAAGTATGCATAATTGATTATTACTTGTGGTGTGGCTATTCGTAAATCCAAAATCCGTTCATAAACTGCCCGAGTAGACTCCAAATCACCTAGACTCTCTTCCAAATCAACATAAAAGGTCCACAATCTCAGAGATTTATGAAGCTTCATCTGAACCGGTTGAT
TARGET=65,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2883
PRIMER_LEFT_PENALTY=0.241544
PRIMER_RIGHT_PENALTY=0.046801
PRIMER_LEFT_SEQUENCE=GCAGAAGGGACATCTTTTTGAG
PRIMER_RIGHT_SEQUENCE=GCTGACAAGTCAGTGACCAAAG
PRIMER_LEFT=29,22
PRIMER_RIGHT=207,22
PRIMER_LEFT_TM=60.242
PRIMER_RIGHT_TM=59.953
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.2883
PRIMER_LEFT_1_PENALTY=0.241544
PRIMER_RIGHT_1_PENALTY=0.046801
PRIMER_LEFT_1_SEQUENCE=AGCAGAAGGGACATCTTTTTGA
PRIMER_RIGHT_1_SEQUENCE=GCTGACAAGTCAGTGACCAAAG
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=207,22
PRIMER_LEFT_1_TM=60.242
PRIMER_RIGHT_1_TM=59.953
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.2883
PRIMER_LEFT_2_PENALTY=0.241544
PRIMER_RIGHT_2_PENALTY=0.046801
PRIMER_LEFT_2_SEQUENCE=CAGAAGGGACATCTTTTTGAGC
PRIMER_RIGHT_2_SEQUENCE=GCTGACAAGTCAGTGACCAAAG
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=207,22
PRIMER_LEFT_2_TM=60.242
PRIMER_RIGHT_2_TM=59.953
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=0.2883
PRIMER_LEFT_3_PENALTY=0.241544
PRIMER_RIGHT_3_PENALTY=0.046801
PRIMER_LEFT_3_SEQUENCE=AGAAGGGACATCTTTTTGAGCA
PRIMER_RIGHT_3_SEQUENCE=GCTGACAAGTCAGTGACCAAAG
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=207,22
PRIMER_LEFT_3_TM=60.242
PRIMER_RIGHT_3_TM=59.953
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=0.3510
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=0.109407
PRIMER_LEFT_4_SEQUENCE=GCAGAAGGGACATCTTTTTGAG
PRIMER_RIGHT_4_SEQUENCE=GGCTGCTTTAGAAAGGCAATTA
PRIMER_LEFT_4=29,22
PRIMER_RIGHT_4=238,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=59.891
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_00884 Ps_contig_mira-and-tgicl-ass_franssen_8477
SEQUENCE=GAACCTTACGCACACGAAKSKGRGAGGCAAACGCGACTTCGACTCAGAGCTTGGCGGTGTGTTGCGATCCGTCCTTCATCTCATGAGTTCATCTTCTTCAACGACGGATCAAAACAAAACTCAGATAATCAGCGTCGCGAAATCAATCTCGACCGTTCGATTTGATTAGCCTGCAAACGCACGAAAATGAATGCGAAATCGAAAGAAAGGATCGCTGGATTGAAGCTCGATTGAGGACAGGTGTCTGGATTCTAACAAGTATATCTTCTTCTTCTAACACTCCTGGCAACGAATCGGACTTTCGTCACGAACAGGCGGAGTTTGTTGTGAATTGGAAATGGAGGTTTGATGAAGGTGATTGTGAATTGAAAAACGGTTACGGTTACGGTGGTTGAAGGTTTTGGTTGAAGAATTGGAAGAGAGTTTTTGATGAAGGTGATTGTGAATTGAAAAACGGTTACGGTTACTGTGGTTGAAGGTTTTGGTTGTAGAAGATTAGGTGATGGAGGAGGGAGTTTTGNGAAGGTGGTTGATGATGGAGGTTGATTGTTCTTNTATTGATGATAGTT
TARGET=264,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00885 Ps_contig_mira-and-tgicl-ass_franssen_8478
SEQUENCE=AAACGGTTACGGTTACTGTGGTTGAAGGTTTTGGTTGTAGAAGATTAGGTGATGGAGGAGGGAGTTTTGTGAAGGTGGTTGATGAATGGAGGTTGAAGAAGGAGAAGCTKRMRGYGGTTGAAGGTTTGGCTATGGAGTTTTGGTGAAGGAATTGAAGAGAGGAAGAAGAAGAAGAAGAATTTGAGAGAGGGAAGGAGAATTTATAGAGTT
TARGET=162,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00886 Ps_contig_mira-and-tgicl-ass_franssen_8497
SEQUENCE=AATTGGAGTACTCTTAGGAGTAGTTTCCAAATTAGGGCTTTCATCAAAGGATTTCAAAGGYGTAGTAGGTAATAAAGCTAAACCAGACTCAAAAGTCCTGCAATTAGTCCCTATAAAGTCTTTGGAAGATGAATACTCAAATCTACCATGGACTTGTGGCTTAGTACTGTTACAGAATTGCTCCTTATGCTGYTCAACAACAACAAGTGTCTCCATAATCACAAGRACTAAGTCAAAAAGCTAGATTTCAAATCTCAGTGCCAAAGTAAGAAAGTACAAAACTTCACCTTGCCAAAACAGATCTCAAACCAACATAAACTTTCTCAGACACAGTTTGTTGCAACTTGCAAGAGATTTTTTTTCCAGAAAGAACACAGAAAGTGTA
TARGET=195,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00887 Ps_contig_mira-and-tgicl-ass_franssen_8507
SEQUENCE=CGACAGTTGTGGAGGGCAGCTGCGGCGGAAGCAGCAGTCTTCTTGGCAGCTCCCTTCCGCCGCAGCCGCCATGAGCGCTCTGCCGGCAAATGCTCCGAAAACCCTAGCGTGCACCATGATCGCTCCGAGAAAATGCTTTTCGTTTTCAAATTTACTAAACCCTAGAGAGAGAGAGAGTATTTTTTCAAGCTTTTAGCCGTTTAGTTCCCTTTCCCGTTTATTGAGCTCKATGTTCACTC
TARGET=164,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00888 Ps_contig_mira-and-tgicl-ass_franssen_8508
SEQUENCE=GTTTCTTCTACGACAGTGTGGAGGTAGCTNCGGCGGAAGCAGCAGTCTTTCTTGGCAGCTCCCTTCGCCGCAGCCGCCATGAGCGCTCTGCCGGCAAATGCTCCGAAAACCCTAGCGTGCACCATGATCGCTCCGAAGAAAATGCTTTTTCGTTTTCTAAATTTACTAAACCCTAGAGAGAGAGAGATTTTTTCTAAGCTTTTAGTCCGTTTAGTTCCCTTTCCCGTTTATTGAGCTCGATGTTCCCCCGC
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1848
PRIMER_LEFT_PENALTY=0.575411
PRIMER_RIGHT_PENALTY=0.609434
PRIMER_LEFT_SEQUENCE=TCTACGACAGTGTGGAGGTAGC
PRIMER_RIGHT_SEQUENCE=CAATAAACGGGAAAGGGAACTA
PRIMER_LEFT=6,22
PRIMER_RIGHT=232,22
PRIMER_LEFT_TM=59.425
PRIMER_RIGHT_TM=59.391
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=1.5515
PRIMER_LEFT_1_PENALTY=0.575411
PRIMER_RIGHT_1_PENALTY=0.976051
PRIMER_LEFT_1_SEQUENCE=TCTACGACAGTGTGGAGGTAGC
PRIMER_RIGHT_1_SEQUENCE=GAACATCGAGCTCAATAAACGG
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=244,22
PRIMER_LEFT_1_TM=59.425
PRIMER_RIGHT_1_TM=60.976
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=1.5515
PRIMER_LEFT_2_PENALTY=0.575411
PRIMER_RIGHT_2_PENALTY=0.976051
PRIMER_LEFT_2_SEQUENCE=TCTACGACAGTGTGGAGGTAGC
PRIMER_RIGHT_2_SEQUENCE=GGAACATCGAGCTCAATAAACG
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=245,22
PRIMER_LEFT_2_TM=59.425
PRIMER_RIGHT_2_TM=60.976
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=1.5885
PRIMER_LEFT_3_PENALTY=0.575411
PRIMER_RIGHT_3_PENALTY=1.013115
PRIMER_LEFT_3_SEQUENCE=TCTACGACAGTGTGGAGGTAGC
PRIMER_RIGHT_3_SEQUENCE=CTCAATAAACGGGAAAGGGAAC
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=234,22
PRIMER_LEFT_3_TM=59.425
PRIMER_RIGHT_3_TM=61.013
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=1.5885
PRIMER_LEFT_4_PENALTY=0.575411
PRIMER_RIGHT_4_PENALTY=1.013115
PRIMER_LEFT_4_SEQUENCE=TCTACGACAGTGTGGAGGTAGC
PRIMER_RIGHT_4_SEQUENCE=TCAATAAACGGGAAAGGGAACT
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=59.425
PRIMER_RIGHT_4_TM=61.013
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_00889 Ps_contig_mira-and-tgicl-ass_franssen_8518
SEQUENCE=TTCATGCCACAWTGTTCATGGTTGGTAAAATAATGTAGCAACAGAAGCTGACATAGCTGCTAATAGAACAGCTGGTTATGGTTTGGTCACAAACATAATCAATGGTGGATTGGAATGTGGAATTCCTGGTGATGCAAGAGTCAACGATCGAATTGGATTTTTTCAAAGATATTCTAAGTTGTTTAATGTTGATGTTGGACCTAACTTGGATTGTGCATATCAAGAAATTCCTTAATAATCTATTTAGTTACTTAAACTTTTAACGTTCCCGGGACGTAAGGGTTTAGTTTGTTTGTTTTTCCTTCTTTCTTTCGTATTTCCTACCAACAACGTAACCTTACTAAATTTAACTTTACCTAACCTAATTAA
TARGET=287,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00890 Ps_contig_mira-and-tgicl-ass_franssen_8519
SEQUENCE=AACCCNCTTCACTTCCNCCGCTTCCTTGTCTAAGCTCACCAACAACGACACCTGCACATACTTCCAGCTCGCGAAGGAGACATTGGATGCTGCTTTGCCTGAAGGGTTGCCGATTGGTATGACGAAGGAGTTTCAGGACTCTATGCGAACTGCGTTGCTAGTTCGCCGGAGCTTTTTGGATCTTCGTGATAACTTCAGGCGTGTGGTTGATCCACCAATGTGGTCACCTCATGGTAAAGGGGTTAAAGTTAGGAAGCAAGTTGTTTTAGATGGTCCTGTTAGTTGTGGGAAGAGCATTGCGCTTGCAATGCTTGTTCAGTGGGCTAGAGAAGAAGGTTGGTTGGTTTTCTATGTTCCCAGAGGAAAGGAATGGACACATGGAGGATTTTTCTATAAGCATCCACAAACAGGTCTATGGGACACACCTGTCCAGGCCGAGTATGTCCTCAAAGATTTCTTGAAGTACAATGAATCCTACCTAAAGCAACTGCCATGCCAAATATTTGATCCAATCCCATTAGGCGAGGGTGCTGGTGTGGGATGGTTAAAAGATGTTGATTCCATGGCAGTTCCTGAAGGTACAATGTTGTATGAGCTGGTGAAGACTGGCATTGAGCAAACACATGCAGCTGTTGGGGTGGTAGTTCGTTTGAGGAAAGAGTTATCACTTGTTAAAGACATGCCTGTTCTTATTGCAATTGATCAATATAATAACTGGTTTACATTCAGTGAGTATGAGGAACCTGTCACAATTCGATCTTGTCGGCCAATACATGCTAGAGAAATTACGATGGTGAATGCTTTTAGGTCAATGATGCATGATAATATGATGGTAGGGGCTTTTTCTCATTCGACAGCTGTAGGAAAGCTTCGTAAAGACTTACCAGATGTACCAGTCGATGCCCGTGCTATGTTTCCTCGATACAGTTTAGAGGAAGCAGACACTGTTTGTCATTATTATTTAAGGCAAAGGCTTATTCGGCGCGAAGCATTCACAGAAGAAAATTGGAAAAAAATTTACTTCCTATGCAATGGAAATGGAACAGAGATGAGGGGGTTAGTTCCTTTCATGCGATGACAGACGTCAGATGCACTAGTATTAATTATTTTTAACCAGTGAGATGTGTTCTGACTGTCATCCATAATAATATAGATATTTTGTATTTTCTTGAGCTATATTCATCATAGAGGATAGAATACAGTTGGATTACTATTTGCACGATCCACTTTAATCTGTTTAATTTGCTTTAAAACTTGTTCTCAAAGGCAGTGTATTGAATGTTTGTTGCAGATTGTGTACATCTAAAAACTTATTTATTACAAAAATGTTAAATTTAGTTGAC
TARGET=335,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0625
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.060196
PRIMER_LEFT_SEQUENCE=GGTATGACGAAGGAGTTTCAGG
PRIMER_RIGHT_SEQUENCE=AAAATCCTCCATGTGTCCATTC
PRIMER_LEFT=115,22
PRIMER_RIGHT=388,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.060
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.0625
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.060196
PRIMER_LEFT_1_SEQUENCE=GGTATGACGAAGGAGTTTCAGG
PRIMER_RIGHT_1_SEQUENCE=GAAAAATCCTCCATGTGTCCAT
PRIMER_LEFT_1=115,22
PRIMER_RIGHT_1=390,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.060
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.0698
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.067516
PRIMER_LEFT_2_SEQUENCE=GGTATGACGAAGGAGTTTCAGG
PRIMER_RIGHT_2_SEQUENCE=TCCTTTCCTCTGGGAACATAGA
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=368,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=60.068
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=0.1532
PRIMER_LEFT_3_PENALTY=0.093026
PRIMER_RIGHT_3_PENALTY=0.060196
PRIMER_LEFT_3_SEQUENCE=AGGAGTTTCAGGACTCTATGCG
PRIMER_RIGHT_3_SEQUENCE=AAAATCCTCCATGTGTCCATTC
PRIMER_LEFT_3=125,22
PRIMER_RIGHT_3=388,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=60.060
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=0.1532
PRIMER_LEFT_4_PENALTY=0.093026
PRIMER_RIGHT_4_PENALTY=0.060196
PRIMER_LEFT_4_SEQUENCE=AGGAGTTTCAGGACTCTATGCG
PRIMER_RIGHT_4_SEQUENCE=GAAAAATCCTCCATGTGTCCAT
PRIMER_LEFT_4=125,22
PRIMER_RIGHT_4=390,22
PRIMER_LEFT_4_TM=59.907
PRIMER_RIGHT_4_TM=60.060
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.1000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_00891 Ps_contig_mira-and-tgicl-ass_franssen_8543
SEQUENCE=GTCCCCCNCTTTCTTTCTCTCTCTATTTATATATATATAGATTGAGGGAGTTTCTAGAGAGAGAAAGAGKAGATTAGTCCATATCTACAGAGAGAGAATCGATTTTGGTTCTTTTTGGGTGACTTTTGTTGTGGGTTTGGTTTGGTTCCRTMKKSGGAGTCTTCTTTGTTGTTGTTGTTCGTGTTATTCTTATGTTTGATGTGTTCGGTCTTTGAAGCCAAGATCTAGTTGAGTGTTGGAGATTTTAGTGTGGTTAAGATGTCATCTCTGAGTAGGGAATTGGTGTTCTTGATCTTGCAGTTTCTTGATGAGGAAAAGTTCAAAGAAACTGTTCACAAGCTTGAGCAGGAGTCTGGTATTTTTCTTAACATGAAGTTTTTTGAGGTATGAAGTT
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00892 Ps_contig_mira-and-tgicl-ass_franssen_8543
SEQUENCE=GTCCCCCNCTTTCTTTCTCTCTCTATTTATATATATATAGATTGAGGGAGTTTCTAGAGAGAGAAAGAGKAGATTAGTCCATATCTACAGAGAGAGAATCGATTTTGGTTCTTTTTGGGTGACTTTTGTTGTGGGTTTGGTTTGGTTCCRTMKKSGGAGTCTTCTTTGTTGTTGTTGTTCGTGTTATTCTTATGTTTGATGTGTTCGGTCTTTGAAGCCAAGATCTAGTTGAGTGTTGGAGATTTTAGTGTGGTTAAGATGTCATCTCTGAGTAGGGAATTGGTGTTCTTGATCTTGCAGTTTCTTGATGAGGAAAAGTTCAAAGAAACTGTTCACAAGCTTGAGCAGGAGTCTGGTATTTTTCTTAACATGAAGTTTTTTGAGGTATGAAGTT
TARGET=166,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00893 Ps_contig_mira-and-tgicl-ass_franssen_8560
SEQUENCE=TTCTGATTGTGGCGAGTGTGTTAAATCTGCTGTTCAAAGAGCTCAAGTTGAATGTGGAAGCTCTATTTCTGGACAAGTTTATTTGCATAAGTGCTTTATCAGTTTTAGCTATTATCCCAATGGGGTTCCAAGGCAACACTCTTCTTATGGTGGTTCATCTTCTTCTTCTTCTTCTGGTTCTTCATCTTTTACTGGACAAAATACAGGGAAGACAGTGGCTATAATATTAGGAGGTA
TARGET=158,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5380
PRIMER_LEFT_PENALTY=0.064105
PRIMER_RIGHT_PENALTY=0.473868
PRIMER_LEFT_SEQUENCE=TCTGCTGTTCAAAGAGCTCAAG
PRIMER_RIGHT_SEQUENCE=GCCACTGTCTTCCCTGTATTTT
PRIMER_LEFT=25,22
PRIMER_RIGHT=218,22
PRIMER_LEFT_TM=59.936
PRIMER_RIGHT_TM=59.526
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=0.5933
PRIMER_LEFT_1_PENALTY=0.064105
PRIMER_RIGHT_1_PENALTY=0.529160
PRIMER_LEFT_1_SEQUENCE=TCTGCTGTTCAAAGAGCTCAAG
PRIMER_RIGHT_1_SEQUENCE=TGTCTTCCCTGTATTTTGTCCA
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=213,22
PRIMER_LEFT_1_TM=59.936
PRIMER_RIGHT_1_TM=59.471
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=0.7972
PRIMER_LEFT_2_PENALTY=0.323355
PRIMER_RIGHT_2_PENALTY=0.473868
PRIMER_LEFT_2_SEQUENCE=TTGTGGCGAGTGTGTTAAATCT
PRIMER_RIGHT_2_SEQUENCE=GCCACTGTCTTCCCTGTATTTT
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=218,22
PRIMER_LEFT_2_TM=59.677
PRIMER_RIGHT_2_TM=59.526
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=213
PRIMER_PAIR_PENALTY_3=0.8525
PRIMER_LEFT_3_PENALTY=0.323355
PRIMER_RIGHT_3_PENALTY=0.529160
PRIMER_LEFT_3_SEQUENCE=TTGTGGCGAGTGTGTTAAATCT
PRIMER_RIGHT_3_SEQUENCE=TGTCTTCCCTGTATTTTGTCCA
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=213,22
PRIMER_LEFT_3_TM=59.677
PRIMER_RIGHT_3_TM=59.471
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.8941
PRIMER_LEFT_4_PENALTY=0.064105
PRIMER_RIGHT_4_PENALTY=0.829981
PRIMER_LEFT_4_SEQUENCE=TCTGCTGTTCAAAGAGCTCAAG
PRIMER_RIGHT_4_SEQUENCE=AGCCACTGTCTTCCCTGTATTT
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=219,22
PRIMER_LEFT_4_TM=59.936
PRIMER_RIGHT_4_TM=59.170
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_00894 Ps_contig_mira-and-tgicl-ass_franssen_8593
SEQUENCE=TGRGAGAAACAAAATATCAATAACATAAGATGGGTTTGTGTTCAATTATTGTCGTTGTTGTTTTGATTGTTTGTTTGTGCGGAGAAGGTTTAGGTTTGAGAGAACAAGAAGAGGATAGAGTTTATGAACTACCAGGGCAACCTTTGGTGAATTTCAAACAATATTCAGGTTATATAACAGTGAATGAGAGTCATGGAAGAGCACTTGTTTTATTGGTTCTTTGAATCTGTTGATCAACCTCAAACAAAACCT
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00895 Ps_contig_mira-and-tgicl-ass_franssen_8595
SEQUENCE=GTCATATAATTAATTAATTAAATAACTAATTAATTAATTAAAATACTTTAAAATTAAAGCTCATTAAATACACACAAGACATCTCTATAGTCCATATAAATGGTAAAAGAGTCGTACACCTTTCCATCCCTACTTTTCTTGTACTCAAACAAGAGATATGAGAGATTAAATGCTGTTAGTTTGCCAAGTCTCTATCCCTGAAAATACTCCAAAGTCCAAACAGGAGGTGCTGCAGTGAAATCTGACAAGTGACTTCCCCCTCCACTGACAACCACATGAATTGTTCTGTTCACAGTTCCAAAGTAATGATATTTTTCTTTATTCACGCATTGGTTCAAAAGTTAGAAGAGTAATTGAAGGGACGATGTGCTGCGAAGATCAATCATGGCTTGTTTGTTTTGATCTACTATTTGACAAGACTAA
TARGET=7,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00896 Ps_contig_mira-and-tgicl-ass_franssen_8595
SEQUENCE=GTCATATAATTAATTAATTAAATAACTAATTAATTAATTAAAATACTTTAAAATTAAAGCTCATTAAATACACACAAGACATCTCTATAGTCCATATAAATGGTAAAAGAGTCGTACACCTTTCCATCCCTACTTTTCTTGTACTCAAACAAGAGATATGAGAGATTAAATGCTGTTAGTTTGCCAAGTCTCTATCCCTGAAAATACTCCAAAGTCCAAACAGGAGGTGCTGCAGTGAAATCTGACAAGTGACTTCCCCCTCCACTGACAACCACATGAATTGTTCTGTTCACAGTTCCAAAGTAATGATATTTTTCTTTATTCACGCATTGGTTCAAAAGTTAGAAGAGTAATTGAAGGGACGATGTGCTGCGAAGATCAATCATGGCTTGTTTGTTTTGATCTACTATTTGACAAGACTAA
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00897 Ps_contig_mira-and-tgicl-ass_franssen_8599
SEQUENCE=GTCGTAGAAACCAAACCTAACTACATATCGTTCTTTACGACGGAACGTTAAAGTTAGTATTAATTAACTACTTACTTTACGACGTCGTCGACATTTCAGTTGCATTTCCAAATGTAGCATTGAGCAAACCCCCAACTGTTGATCCGGTGTAGAAGGCAAGTTGTTCGGTAGCATAACCTAGACGTTCAGCCAATGGAGCAATCCCCAATAAAGCGAAGAAGAAAACCCAAACATGTTTAGCAGTGAAATAATGCAGGAAGATAGCCAAGGGGCCAAAAGGTAACAAGATGTTGATCTTAGCTTTGATTAAAACAGTATAAATACTCCTACTGATTCGTCTAGTCGAAGAAGAAGATGATGATTTGGGTAAAGGAAGTGATGAATCGAAATGAAAAGTATGAGGTTTAGATGCAGTAGTAGAAGAAGAACTGAGAGGAGTTTCCTCATCGAAATCAGTCTCCATCTTATGCTCCGCCATCTCACAGATCAGATCAGATCAACTCAACTCAACTCAAACCCAGTTTGACTGTAACACAATACAGCTGGACCCCACACCACAATATACTAACAACTTCTTAATCTCTTTTTTTTTTCTATGAATTCATTCATATTTGGCGTTGATTTGAATTCAAT
TARGET=483,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2043
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=0.129818
PRIMER_LEFT_SEQUENCE=AGGAGTTTCCTCATCGAAATCA
PRIMER_RIGHT_SEQUENCE=AGTATATTGTGGTGTGGGGTCC
PRIMER_LEFT=433,22
PRIMER_RIGHT=565,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=0.2043
PRIMER_LEFT_1_PENALTY=0.074466
PRIMER_RIGHT_1_PENALTY=0.129818
PRIMER_LEFT_1_SEQUENCE=GGAGTTTCCTCATCGAAATCAG
PRIMER_RIGHT_1_SEQUENCE=AGTATATTGTGGTGTGGGGTCC
PRIMER_LEFT_1=434,22
PRIMER_RIGHT_1=565,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.870
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=0.2945
PRIMER_LEFT_2_PENALTY=0.164639
PRIMER_RIGHT_2_PENALTY=0.129818
PRIMER_LEFT_2_SEQUENCE=GGGTAAAGGAAGTGATGAATCG
PRIMER_RIGHT_2_SEQUENCE=AGTATATTGTGGTGTGGGGTCC
PRIMER_LEFT_2=364,22
PRIMER_RIGHT_2=565,22
PRIMER_LEFT_2_TM=59.835
PRIMER_RIGHT_2_TM=59.870
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.3032
PRIMER_LEFT_3_PENALTY=0.173354
PRIMER_RIGHT_3_PENALTY=0.129818
PRIMER_LEFT_3_SEQUENCE=GAGAGGAGTTTCCTCATCGAAA
PRIMER_RIGHT_3_SEQUENCE=AGTATATTGTGGTGTGGGGTCC
PRIMER_LEFT_3=430,22
PRIMER_RIGHT_3=565,22
PRIMER_LEFT_3_TM=59.827
PRIMER_RIGHT_3_TM=59.870
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.0000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=0.5935
PRIMER_LEFT_4_PENALTY=0.463680
PRIMER_RIGHT_4_PENALTY=0.129818
PRIMER_LEFT_4_SEQUENCE=CCTCATCGAAATCAGTCTCCAT
PRIMER_RIGHT_4_SEQUENCE=AGTATATTGTGGTGTGGGGTCC
PRIMER_LEFT_4=441,22
PRIMER_RIGHT_4=565,22
PRIMER_LEFT_4_TM=60.464
PRIMER_RIGHT_4_TM=59.870
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_00898 Ps_contig_mira-and-tgicl-ass_franssen_8599
SEQUENCE=GTCGTAGAAACCAAACCTAACTACATATCGTTCTTTACGACGGAACGTTAAAGTTAGTATTAATTAACTACTTACTTTACGACGTCGTCGACATTTCAGTTGCATTTCCAAATGTAGCATTGAGCAAACCCCCAACTGTTGATCCGGTGTAGAAGGCAAGTTGTTCGGTAGCATAACCTAGACGTTCAGCCAATGGAGCAATCCCCAATAAAGCGAAGAAGAAAACCCAAACATGTTTAGCAGTGAAATAATGCAGGAAGATAGCCAAGGGGCCAAAAGGTAACAAGATGTTGATCTTAGCTTTGATTAAAACAGTATAAATACTCCTACTGATTCGTCTAGTCGAAGAAGAAGATGATGATTTGGGTAAAGGAAGTGATGAATCGAAATGAAAAGTATGAGGTTTAGATGCAGTAGTAGAAGAAGAACTGAGAGGAGTTTCCTCATCGAAATCAGTCTCCATCTTATGCTCCGCCATCTCACAGATCAGATCAGATCAACTCAACTCAACTCAAACCCAGTTTGACTGTAACACAATACAGCTGGACCCCACACCACAATATACTAACAACTTCTTAATCTCTTTTTTTTTTCTATGAATTCATTCATATTTGGCGTTGATTTGAATTCAAT
TARGET=498,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2043
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=0.129818
PRIMER_LEFT_SEQUENCE=AGGAGTTTCCTCATCGAAATCA
PRIMER_RIGHT_SEQUENCE=AGTATATTGTGGTGTGGGGTCC
PRIMER_LEFT=433,22
PRIMER_RIGHT=565,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=0.2043
PRIMER_LEFT_1_PENALTY=0.074466
PRIMER_RIGHT_1_PENALTY=0.129818
PRIMER_LEFT_1_SEQUENCE=GGAGTTTCCTCATCGAAATCAG
PRIMER_RIGHT_1_SEQUENCE=AGTATATTGTGGTGTGGGGTCC
PRIMER_LEFT_1=434,22
PRIMER_RIGHT_1=565,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.870
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=0.2945
PRIMER_LEFT_2_PENALTY=0.164639
PRIMER_RIGHT_2_PENALTY=0.129818
PRIMER_LEFT_2_SEQUENCE=GGGTAAAGGAAGTGATGAATCG
PRIMER_RIGHT_2_SEQUENCE=AGTATATTGTGGTGTGGGGTCC
PRIMER_LEFT_2=364,22
PRIMER_RIGHT_2=565,22
PRIMER_LEFT_2_TM=59.835
PRIMER_RIGHT_2_TM=59.870
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.3032
PRIMER_LEFT_3_PENALTY=0.173354
PRIMER_RIGHT_3_PENALTY=0.129818
PRIMER_LEFT_3_SEQUENCE=GAGAGGAGTTTCCTCATCGAAA
PRIMER_RIGHT_3_SEQUENCE=AGTATATTGTGGTGTGGGGTCC
PRIMER_LEFT_3=430,22
PRIMER_RIGHT_3=565,22
PRIMER_LEFT_3_TM=59.827
PRIMER_RIGHT_3_TM=59.870
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.0000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=0.5935
PRIMER_LEFT_4_PENALTY=0.463680
PRIMER_RIGHT_4_PENALTY=0.129818
PRIMER_LEFT_4_SEQUENCE=CCTCATCGAAATCAGTCTCCAT
PRIMER_RIGHT_4_SEQUENCE=AGTATATTGTGGTGTGGGGTCC
PRIMER_LEFT_4=441,22
PRIMER_RIGHT_4=565,22
PRIMER_LEFT_4_TM=60.464
PRIMER_RIGHT_4_TM=59.870
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_00899 Ps_contig_mira-and-tgicl-ass_franssen_8611
SEQUENCE=AGTGATGCAGATTCAGTTTCCCTATTCAGGAAAGGTTTAGAAGAAGCCARCAAGTTTCTTCCTCCGCAGCCTAAGCTCGTGACCGGTCTAGAATCATCCAATTTCAATATGGTTGGAGAGAAAGAGAATCGTTGTGTTGAAGGGTAGAAAGAATCACGAGCGCGTAAGAAAGTGATAGGTAAAAGAAGAACAAGAAGAAGAAGATAGGAAGAAGTAAACAAGCAATACAGCTAAGG
TARGET=192,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00900 Ps_contig_mira-and-tgicl-ass_franssen_8612
SEQUENCE=CCGTTTTTACTTGTCCGTTCTGTAAGCTATCATTTTTCATTTGCAGAGTGGCAAATGCTCCACACTAAGCAAAACTTTATCGAACATCTCTGATAATTCATCTTCATCAACAACACTAACTGCTGATTGCTTGTTACTTCTCCTATCTTCTTCTTCTTGTTCTTCTTTACCTATCACTTTCTTCGCGCTCGTGATTCTTTCTACCCTKCAACACAAACGGATTCTCTTTCTCTCCAACCATATTGAAATTGGA
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00901 Ps_contig_mira-and-tgicl-ass_franssen_8620
SEQUENCE=ACTTTTTTACTATCGTTHTTAAATAAAATTTCAATCAAATTGATACATCAGTATACATTGTACACATTGTTTCGGAATGCTTAGACCATACCTCAAACTACATGCAAGGCTAAACTGTATTTTTCATCCAATGTAGCAGTTGATTTTGAACTCTTGGTTTACTAATGCAAAAAATAATACAATTCTCTATTTGCAACAGACAAATTACAGATGAAAATGGAATCTTACATCCAAAAGCAACTAAAAAATGGAACAACTTCGAAATGGATGTTTAGTGATTTGGTCAGGTGTTTGCTTTGTTTTATGGAAAAAAACTCCGAGTGGCTTGCTTCTTTGCAGGATGGCTGTCAGGGTCATATGCTTCCATGAAGGGAGCAGGAAGGCCAAGTCTTCCCTCATTTGAATTAGTGCCATCTTCCGGACCTACCCAAGCCAGAGGTCTCACTTGCTCAGGTTCAACTGATTTAGCATGAGAGCGCTTCCTCACATAGTTTTCTTGCAATGGCTTAGGTATTTGAAATGAGAACCGTGAAAGAGTTACGCCGTCTATCATGTACTCTGGATGTTCCACCAAGAACTTCTGGACCTCGACCATTGAGCGCAATCTCTTGCCTGATGGTGCTACATAGTATATATCTGCAAATTTGCTGCTTCCTTCACCTCTAATTCGTAGAAGTCGCTGCCATCCATCTGGAGGCTGTGCAATGCTAGGCTTGTCAATAGCCCAAATTCTACTGCCATCCTGGGAAATATCCTCGGGATCATCACAAGATATATCGGGTCGCCACTCACGAGCTTTTTCACAAACAAAAGGATGTTGAAGGATATACTCGCGTATTTCTTCGTATTTTTCCTTTGTTGGAATCAACCTCCATTTAAAACAGGAAGCACATTGAACAGTGAAAGCACCAACTGCTGGCAAAACTCTAGGAGTTGAACTTGAAGGTTTACTTCGTGGACGCGGTGGAGACTTAAACTGAAAAGGATCCGGGGATTGAATAGTGTTGTCTCCATTTATCACAGGGTCATAAAGTACTAATTGTTTAGATGCGTCGTCATTAGAGATGTTATTTTCATCATCTGAAGATGAAGAAGAAACATATATGGGTTCGGGAAGACTTCTATGTTGATTATCAGGAAAACCTGAACCTGCTAGGTCATTGACTTGATTCTTGGGTGTAAGTGAAAACTTCCCACGATGCATTTGATTTTCCTAGGTCTAGCACCTGATACACTAGAGGGAGGTAGATAGAAGAAGAAGAAAGAGGCTGACTAAAAAGTTAAGATCCTGTTGCTRKAAAMCTTAAAAGAAGAAACNCAGAGAGCGTTGAAGGNTGAACTGAACAAAGTNTTTA
TARGET=1251,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00902 Ps_contig_mira-and-tgicl-ass_franssen_8627
SEQUENCE=GGTTTACTAAATGATAGTTCTATTGAGAATAAAAGTGTTCCTTTTCTTCTTCAAATATTGAGTTCACAATTTGGTTTACAAAATAAAATTTTTTTAGGTTCACTTATCAATGTAAAATACTACCAGAAAACTAATTTTGAATTCTTCTTCTTCATATCAGCTTCTCTGAATGATTTTTCGACAAGGCCGGACAGGGTCAATTTCAAATCATGGGTCATCTTGTGAAGAATCAACGATAATGTGTTCACATCTACCATGGCTCTGTGGGCTGAGCCATCCACTTTAATATCATAGAACTTGGTAAGGGCATCAAGGGATACTGAAGGAAGTTTTGTTCCTTTAGGCTTTAGTTGACGTGCTAAAGGAAGACTGTCCAGAAACAGCCAATTCAGAGGAATCGGTGTCGAACATCGACGTAACTCGTTAATGATGAACGGTACATCAAAAGTACGAGCATTATGAGCAACCCATAACACGTATCCCCCAGGTTTCTCACGGCTTTTAACGTAGCTTAATAATATGGGAACCAGATCTTCCATCCTCGGAACACCAGGTCTGTTCACCATATCTGTAGTAATGCCATGGATTTTTGTAGTTTTCAACACAGCATTGAGGATTTACAAGAGTTTCGAAAGTGCTGTTGGGACCACCCTGAAGATCTCGAAGCGCAATCTCAATGATCCTTTCTTTCTCTCGGTGAAGCCCGGTGGTCTCAATATCAAAAACAATAACAGTGACTAAGCTAGACAAGTCTTTATTCTGAGCTAACTCTTGTTGAATGTTACATTGCTGAATTTCCTGAAATTGACCGAGCTGTGTTTTATTTTACATTTACATTAGAGCTAGTTGAAACTGTTTCACTCAAAATTTCTAGCTTGGTGCTTCTTGATCTGGTGTCCGCGCTGCTTTCTGTGGTTGTAGTTATCGGTCTTCGAGTCCACTTTTTTCTGTGGCCTCCCTGAAGTCCAATAAATTCTAGATCCAAGCAACCTAACACTA
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00903 Ps_contig_mira-and-tgicl-ass_franssen_8629
SEQUENCE=GTCGAAAAATACATTCAGAGAAGCTGATTATGAAGAAGAAGAATTCAAAATAGTTTTCTGGTAGTATTTTACATTGATAAGTGACCAAAAAATTTATTTGTAACCAAATTGTGAACTCAATATTTGAAGAAGAAAAGGAACACTTTTATTCTCAATAGAACTATCATTTGTACC
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00904 Ps_contig_mira-and-tgicl-ass_franssen_8642
SEQUENCE=CTAATCTCTATCTGGTTTTGTTTAGTAGGAAACGAATTATTATGTTATGATTTTTATTCAAGAGGAAGCCTAGAAGAAAACCTACACGGTAACAGAATTAACTATAATTCATTTGGCTGGCAAGAGAGGTACCGAGTGGCTGTGGGTGTAGCCGAGGCCTTGGATTATCTGCATAATGGCTGTGCCCGAGCTGTAATCCATAGGGATGTCAAATCTTCTAATATCCTTCTTTCAGATGATTTTGAGGCTCAGCTCTCAGATTTTGGACTGGCTAGTTGGGGTTCTTCTTCTTCTCATATTTATTTTGTACTGATGGTTGCCGGAACTATTTTGGGTACCTAGCTCCTGAATTNTTTTATGCACGGTAGAGTTACTAAGAAAAAATTGATGTATATGCTAGTTTTGGTTAAGGTTTGTTACCTTTCCG
TARGET=282,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1727
PRIMER_LEFT_PENALTY=0.050443
PRIMER_RIGHT_PENALTY=0.122257
PRIMER_LEFT_SEQUENCE=TGGATTATCTGCATAATGGCTG
PRIMER_RIGHT_SEQUENCE=TTCAGGAGCTAGGTACCCAAAA
PRIMER_LEFT=160,22
PRIMER_RIGHT=349,22
PRIMER_LEFT_TM=59.950
PRIMER_RIGHT_TM=60.122
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=0.7849
PRIMER_LEFT_1_PENALTY=0.674496
PRIMER_RIGHT_1_PENALTY=0.110393
PRIMER_LEFT_1_SEQUENCE=GCCTAGAAGAAAACCTACACGG
PRIMER_RIGHT_1_SEQUENCE=CGGCAACCATCAGTACAAAATA
PRIMER_LEFT_1=67,22
PRIMER_RIGHT_1=321,22
PRIMER_LEFT_1_TM=59.326
PRIMER_RIGHT_1_TM=59.890
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=0.7968
PRIMER_LEFT_2_PENALTY=0.674496
PRIMER_RIGHT_2_PENALTY=0.122257
PRIMER_LEFT_2_SEQUENCE=GCCTAGAAGAAAACCTACACGG
PRIMER_RIGHT_2_SEQUENCE=TTCAGGAGCTAGGTACCCAAAA
PRIMER_LEFT_2=67,22
PRIMER_RIGHT_2=349,22
PRIMER_LEFT_2_TM=59.326
PRIMER_RIGHT_2_TM=60.122
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.8952
PRIMER_LEFT_3_PENALTY=0.050443
PRIMER_RIGHT_3_PENALTY=0.844757
PRIMER_LEFT_3_SEQUENCE=TGGATTATCTGCATAATGGCTG
PRIMER_RIGHT_3_SEQUENCE=TCAGGAGCTAGGTACCCAAAAT
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=348,22
PRIMER_LEFT_3_TM=59.950
PRIMER_RIGHT_3_TM=59.155
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.9918
PRIMER_LEFT_4_PENALTY=0.869569
PRIMER_RIGHT_4_PENALTY=0.122257
PRIMER_LEFT_4_SEQUENCE=ATTATCTGCATAATGGCTGTGC
PRIMER_RIGHT_4_SEQUENCE=TTCAGGAGCTAGGTACCCAAAA
PRIMER_LEFT_4=163,22
PRIMER_RIGHT_4=349,22
PRIMER_LEFT_4_TM=59.130
PRIMER_RIGHT_4_TM=60.122
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_00905 Ps_contig_mira-and-tgicl-ass_franssen_8644
SEQUENCE=TGATGCTAATGATGAGGATCATAATGCTTCAATGGATGTGAAGCCAAACCCTAAATTACTCTCACTTGAATGGCAGCAAGATCAAGGTTGCTCTGATGCTGGATATGGATCATGGAGTGGCATGATGAACGGTTATGGATCTTCCACAACAAACCCTTTGATCAACGGCTAAGATTTCTCTTGGTAAATGGTTAATTTGATCAATGACCTAATCAATGACTCTATAGTATTATATATACATAAAGTTATTGTATGGGGAGGGTTTAATTAATTAATTAATCAATTAATTGCATGTCTTTTTTTTATTAGTGTTTTTTAGTNTGGTGGTATGGATGGNGTGTGATGAGAGGTGAGAGTCAATGTACTGNATGGTGTGTAGNTTTGATTATATAATANNNATGTGT
TARGET=264,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2587
PRIMER_LEFT_PENALTY=0.124655
PRIMER_RIGHT_PENALTY=0.134049
PRIMER_LEFT_SEQUENCE=CAAGATCAAGGTTGCTCTGATG
PRIMER_RIGHT_SEQUENCE=TCTCATCACACNCCATCCATAC
PRIMER_LEFT=76,22
PRIMER_RIGHT=347,22
PRIMER_LEFT_TM=59.875
PRIMER_RIGHT_TM=60.134
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.75
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.3945
PRIMER_LEFT_1_PENALTY=0.124655
PRIMER_RIGHT_1_PENALTY=0.269856
PRIMER_LEFT_1_SEQUENCE=CAAGATCAAGGTTGCTCTGATG
PRIMER_RIGHT_1_SEQUENCE=CTCTCATCACACNCCATCCATA
PRIMER_LEFT_1=76,22
PRIMER_RIGHT_1=348,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.270
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.4627
PRIMER_LEFT_2_PENALTY=0.328676
PRIMER_RIGHT_2_PENALTY=0.134049
PRIMER_LEFT_2_SEQUENCE=GATGAACGGTTATGGATCTTCC
PRIMER_RIGHT_2_SEQUENCE=TCTCATCACACNCCATCCATAC
PRIMER_LEFT_2=123,22
PRIMER_RIGHT_2=347,22
PRIMER_LEFT_2_TM=59.671
PRIMER_RIGHT_2_TM=60.134
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=0.5985
PRIMER_LEFT_3_PENALTY=0.328676
PRIMER_RIGHT_3_PENALTY=0.269856
PRIMER_LEFT_3_SEQUENCE=GATGAACGGTTATGGATCTTCC
PRIMER_RIGHT_3_SEQUENCE=CTCTCATCACACNCCATCCATA
PRIMER_LEFT_3=123,22
PRIMER_RIGHT_3=348,22
PRIMER_LEFT_3_TM=59.671
PRIMER_RIGHT_3_TM=60.270
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.6123
PRIMER_LEFT_4_PENALTY=0.478252
PRIMER_RIGHT_4_PENALTY=0.134049
PRIMER_LEFT_4_SEQUENCE=TTACTCTCACTTGAATGGCAGC
PRIMER_RIGHT_4_SEQUENCE=TCTCATCACACNCCATCCATAC
PRIMER_LEFT_4=55,22
PRIMER_RIGHT_4=347,22
PRIMER_LEFT_4_TM=59.522
PRIMER_RIGHT_4_TM=60.134
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_00906 Ps_contig_mira-and-tgicl-ass_franssen_8648
SEQUENCE=AAGAGAAAGAGAGTTTTCTTGCGTTACAAGCACAGACCAAGGCTTGCAAAGCTTTCACGGAATTTCTGAGAAGGTCCGGTTCCGATGATGAAGAAGAGGATAATGATGATGATGACAGTGATGCTTAGTGGTT
TARGET=103,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00907 Ps_contig_mira-and-tgicl-ass_franssen_8653
SEQUENCE=AATAATTGGAGCATTAGGGATTCCCGCATTTCCAACAGTTAACAGGAAATAATTCCATCCTCTTCTATGCACCTGTCATCTTCCAGAGTTTAGGATTCGGAGCTAACGCATCTCTTTTCTCGTCTTTTATTACCAACGGAGCTCTCCTTGTTGCTACTGTCATCTCAATGTTTTTAGTTGACAAGTTTGGTAGAAGAAAATTCTTCCTAGAAGCTGGTTTTGAAATGATATGCTGCATGATTATTACTGCTGTGGTTTTGGCACTGGAGTTTGGACATGGCAAAGWAACTTTCAAAAGGAATAAGTACACTTCTTGTTATTGTTGATTTTCTTTGTTTTGGTTGGTTGGTCCG
TARGET=338,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00908 Ps_contig_mira-and-tgicl-ass_franssen_8675
SEQUENCE=CGTTTTTTTTTAACTAAATTAAGTATCTATTATTTATTTTATTTAATTTTTTTWTKTTTWTTAACAAATTTATTTATTTATTTTTAAAAAGACAAAAAAATTGACATAGATATCACTGGCTTCCCTCTCATATGTTTCATGTAAACTTATTTGATAATACAGTCCTACTGGTAAAAAGAGTTTATTTTAAACCTTCATTTTATTCTAAGCAACTCTCGTTCCACAACAAAGTCACAACATATTACTACTACTTCTCAAGATTCTTGATAACTGGACGAGCTTTACACACAGCCCTTAATGGAACCGATCGAGACAAAGTGAATCCACTTTCTTCACTCATATCAATCTCTTTGTCACCCACTTTTTTCCATTCTAAGCACTGAATCAATAAACCCAAAGTTAGGCTTATTGCACGCAGAGCCAACACTTCACCTGGACAAGCCCTTCTACCCATCCCAAACGCAATCAACTTCTCCAACTCTCCTTCTTTCTCAAACCTCTCAGGCTTGAAGGTTGTTGCCTCACTCCATGTTTCAGGGTCTCTATGAATGGCCCAAGCATTGATCAATACTATGGTGTCTCCTGGTACGTTGTATCCTCCAATAATACAGTTGTCGGAAGATGAGTGTGGTAGTGATAATGGAGCAGGAGTGTACAACCTAAGTGTCTCGTAGATTATGTTTTTAAGGTAAGGGAGTTTTGGAAGGTCGGATTCATCCAATAAACGGTCTTGACCTACGTGAGTTTCCAGTTCTTGTCTCGCCTTCTCTAGCACTTCTGGATAGGTCAATACACAAGATAATGCCCACTCTAAGGTTACAGCTGATGAGTCTGTTCCAGCAAGAAGCATACCCAGAGCAAGACCTTTGATGATTTGATCAGTATAGTACTCAGGTTGTGATTCTTGCAGACTTAAAAGATGATCTATCATAGTATTCGTATGTTGCCCTTTGTTGCGTTGTTCTTGAATCAGTCCTCTCAAGAACGTATCTGTTTTGCTGCTAATCTTCTTTAACCTCTTCTCCAAGTTTCAAAGTC
TARGET=68,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00909 Ps_contig_mira-and-tgicl-ass_franssen_8689
SEQUENCE=GSWCAAAAGATTACAGTTTGGGACAGAAAAAAAGTTAAAGCAAGGTGCAATTTTATACAGGTTTGGTCTCTGACCAGAAATATCACAATTCTGTAAAATTCACAGACTACATAAGTTATTACATATGGAAGATAGCACTGGGTTCACGAAAGAAGAGAGACCAGAGAAACAAACTCCAGCGTAACCCGACCCTTGGACCTTGTGTTTATGACAAAATGCTCCGGGTATTTCCTTAATAGCATAAGTAAGCCATACCAAGGTTTTCCTTTGGATTCAGGACCAGGCCATTCCTCTGATGTGAGATATCTTCTTACTCTACCATGGTGCCAAACACTGCTATACTTATCCACCAACACAGCATGAAGTCTCTCCATAGGCATCCACTCTCCAGGGCCACAAAGATCAGAGAGGACATTAGCTACAGATGATACTACATCCTTTTCTTCCAATTGCTGAAGTTGAAGATTATCAGCATCACGATCTACTCGGGACCTGCCTTCTGCCTTCTTATCAATTGGTCCAGGCAGAGAAGCCTTTCCCCCAGAACCAACTCCACCACCATACCCATAACCATCATAAGCCTCCCTCCTGTTAACCTTCTTCCCACTACTACCACCACCACCAACAACAACATCAACATCAACATCATCATCCATCTCATACTCCTCTTCATAATACTCCCTCCTCTCTTTCTTCACCATCGCGCCACCCGACCCTGCAGGACGTCCATAACCATAATGCGGCTGAACAGGCGGAGGAAGAGCAGGAGAAGGAGCAGGAGGAGCAATAGCTGAGGAATGATGATTAGCATAATGCCGAGTACCAACTGAGCCACCGCCGCCGAGTATACTACGAACAGGAAGMMAAAAAGTAAACTCTTANCACCGATCTAGAAGAAGAATACCTAAGAAGAATACAAGACTTAACGAGGGAGGTACCAGGGAG
TARGET=612,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00910 Ps_contig_mira-and-tgicl-ass_franssen_8704
SEQUENCE=CGCTATGGATTTTCCAGGATTCGGTCTTTCAGAAGGTCTTCATGGATATATTCCAAGTTTCGATCAGCTTGTTGATGATGTCATTGAGCAGTACTCCAAAATCAAAGAAAATCCAGAGTTCAAATCTCTTCCAAGTTTTCTTTTTTGGKACAATCAATGGGTGGAGCTGTTGCATTAAAGATACATCTTAAACAACCAAAAGCATGGAATGGTGCAATTCTTTGTGCACCAATGTGTAAAATTTCAGAGAACATGGTTCCACCAAAGTTGGTTGTTCATATGCTAACCGCAATGGCAAATGTTTTCCCGAAGAAGAAGTTAGTTCCAATGAAAGATATGGGAGACGCGGCATTCAGAGAACCAAAGAAAAAGAAACAGACGGAATATAATGTGATTAGTTACAAGGATAAACCGCGCTTGCGCACCGCGGTAGAACTTCTTAAAACTACCGAAGAAATCGAACAAAACTTGGAAGAAGTGACTCTACCATTATTAATCCTTCATGGAGAAGCTGATACTGTGACTGATCCATCAGTGAGCAAAGCATTGTATGAAAAAGCAACTTCTTCTGACAAGAAACTTGAGCTTTACAAAGATGCTTACCATGCTCTAATTGAGGGTGAACCTGATGAAAGAGTAATTCAAGTTCTTAATGACATCATTTCTTGGCTTGATGAGCACAGCTTGAAACAAAATATTTAATCTTCCTCTTAATATTGATGATGTGGTTACATAAGAAAGAAAGAAAGAAACACTATCATTTATCTTGTTCTCTTACAATTATTTTTTGTTACATTCAATTATACCTGACCATACTTAATGGTGTATATGAATAACATACAATTGTTTATATCCTTACATAAGAAATAAACACAATCATTCATGTTACTTTT
TARGET=735,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00911 Ps_contig_mira-and-tgicl-ass_franssen_8718
SEQUENCE=ATTACTTATATTCTTTGAAGTTGAAATTGAAAGAGGCTTGCTCTGTGAAACCCTAACCCTAAGGGGAGGGAAGAGAGAGAGAGGCGCAATGTCAGTTAGGTTAAGGAATAGTAATAGGAATGATTACAAATTACTCCGATTACGAGACGCTCATTCTTGTATCAATCAGAGAGTTAACATCATAGCCATTGTTCTTGAGTTTGGTCTCCCCAAAACAACTAGAGGCACTGATCAGTGTTGTACCCTAAGATTGATTGATGAAACTCATCACCAAATCGGTATGGCTGTTAATATTTTTACTGAAAATGCTGAAAGTCTTCCCCATGTTGCTGCTGCTGGAGATATAATTCAGCTTTGTAATGTTACGTTAAGGACGCATAATTAAGGAAGTAAATGCTGTTTTCTACAAAAACTCTTC
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1007
PRIMER_LEFT_PENALTY=0.060824
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=AAAGAGGCTTGCTCTGTGAAAC
PRIMER_RIGHT_SEQUENCE=ACCAAACTCAAGAACAATGGCT
PRIMER_LEFT=29,22
PRIMER_RIGHT=204,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.2044
PRIMER_LEFT_1_PENALTY=0.164560
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=CTTGCTCTGTGAAACCCTAACC
PRIMER_RIGHT_1_SEQUENCE=ACCAAACTCAAGAACAATGGCT
PRIMER_LEFT_1=36,22
PRIMER_RIGHT_1=204,22
PRIMER_LEFT_1_TM=60.165
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.2838
PRIMER_LEFT_2_PENALTY=0.243965
PRIMER_RIGHT_2_PENALTY=0.039879
PRIMER_LEFT_2_SEQUENCE=GTTGAAATTGAAAGAGGCTTGC
PRIMER_RIGHT_2_SEQUENCE=ACCAAACTCAAGAACAATGGCT
PRIMER_LEFT_2=19,22
PRIMER_RIGHT_2=204,22
PRIMER_LEFT_2_TM=60.244
PRIMER_RIGHT_2_TM=60.040
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=0.3425
PRIMER_LEFT_3_PENALTY=0.302618
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=GCTTGCTCTGTGAAACCCTAAC
PRIMER_RIGHT_3_SEQUENCE=ACCAAACTCAAGAACAATGGCT
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=204,22
PRIMER_LEFT_3_TM=60.303
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=0.3870
PRIMER_LEFT_4_PENALTY=0.347129
PRIMER_RIGHT_4_PENALTY=0.039879
PRIMER_LEFT_4_SEQUENCE=GCTCTGTGAAACCCTAACCCTA
PRIMER_RIGHT_4_SEQUENCE=ACCAAACTCAAGAACAATGGCT
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=204,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=60.040
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_00912 Ps_contig_mira-and-tgicl-ass_franssen_8723
SEQUENCE=CGTTGGGTACCCGAAACTAGGTTTTCGTTTCCTTTCCTTTCCTTCGGGTAAAGTACTCTTCTGCTCGCAATAGTCCAGTATTGTATACATTAAATAATTTTTCAAATCTTTTCATTGGATGGGAACACCTTGTATATCTTGTGATAGAARACTTCAAGCAGCCGTAGCTCAGCATCAGGATGAGATAACTCCACCTTTGTTTTAAGATCATCAAGGACATCACCAACAGTGCTCTGCTTTGGGAGTCTGATAGTGTGACTAACCACCTCGTCCTTGATGGCGTGATGAAATGCAACTTTCAGAGTTTTCAAGCCTTGTAATTCGGGTAGAGGGATGTCGAGCACTTCATAGTATAATATATCTGATGTCTGATTGTAATGAACTAGCATATCAGACAAAATGATCAACGCCTCGG
TARGET=28,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00913 Ps_contig_mira-and-tgicl-ass_franssen_8758
SEQUENCE=TAAGAGCATATGTGGAGCTGATGGGTATGATGAGATCGGCGGTGGAGACGGGGAGCTTGGCGAACGGGACTGAAACAACGGCGGAAGGTAGGTGGGTGGTGGCGGTGGTGACGGCATGAATTCACAACAACCGTCTAGTGCGTGACCACCTAAAGTTGCCACGTGGTTTTTGAGACATTCTTTGTAAGTAACAGCAACAACGTTGTTGTTGTGGTGGTTGTGAGTGTTGGGATGGTGGCGTTTAAGAACCCCATTTGAAAAAGACAGAGGCTTTGTTATGTTTGAGGTTGTTGTGTTAACGGGTTGTTGTTGTTGGATTCGGGTCGGGTTTTCTGAATCGGGTTCTGATTGGTTTTGAAATTGTTGTCAAAGGAGTAGTGTTTGTGGCGGTTGTTGTTGTTGTTAAAACCTCCATCATGATAAATCTAAAAAAGATTAAAATTAAAAAAACTTACCTTAGTTTCAATTATGTGGTTCTAACTTCTAAGTTTATAAAACCTCATAAATGTATCACATCATACACTATAGTCTCCATGAAGAAGCTAGCTTGGTTTTGAGCTTATTTTTTTCTGAAAATTTGAGGAATTGAAAGGAGAACAAAAAGAAAGAGGAGAAGTGAGTGGTGGAGGAGGAGAAGTGGTGGTGGTTGTGG
TARGET=303,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6375
PRIMER_LEFT_PENALTY=0.102129
PRIMER_RIGHT_PENALTY=0.535412
PRIMER_LEFT_SEQUENCE=CATGAATTCACAACAACCGTCT
PRIMER_RIGHT_SEQUENCE=ATCAGAACCCGATTCAGAAAAC
PRIMER_LEFT=114,22
PRIMER_RIGHT=348,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=59.465
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=0.6849
PRIMER_LEFT_1_PENALTY=0.149516
PRIMER_RIGHT_1_PENALTY=0.535412
PRIMER_LEFT_1_SEQUENCE=GAACCCCATTTGAAAAAGACAG
PRIMER_RIGHT_1_SEQUENCE=ATCAGAACCCGATTCAGAAAAC
PRIMER_LEFT_1=245,22
PRIMER_RIGHT_1=348,22
PRIMER_LEFT_1_TM=59.850
PRIMER_RIGHT_1_TM=59.465
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=104
PRIMER_PAIR_PENALTY_2=0.7797
PRIMER_LEFT_2_PENALTY=0.065292
PRIMER_RIGHT_2_PENALTY=0.714453
PRIMER_LEFT_2_SEQUENCE=TTTGAGGTTGTTGTGTTAACGG
PRIMER_RIGHT_2_SEQUENCE=GCCACAAACACTACTCCTTTGA
PRIMER_LEFT_2=280,22
PRIMER_RIGHT_2=387,22
PRIMER_LEFT_2_TM=59.935
PRIMER_RIGHT_2_TM=59.286
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.8166
PRIMER_LEFT_3_PENALTY=0.102129
PRIMER_RIGHT_3_PENALTY=0.714453
PRIMER_LEFT_3_SEQUENCE=CATGAATTCACAACAACCGTCT
PRIMER_RIGHT_3_SEQUENCE=GCCACAAACACTACTCCTTTGA
PRIMER_LEFT_3=114,22
PRIMER_RIGHT_3=387,22
PRIMER_LEFT_3_TM=59.898
PRIMER_RIGHT_3_TM=59.286
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.8640
PRIMER_LEFT_4_PENALTY=0.149516
PRIMER_RIGHT_4_PENALTY=0.714453
PRIMER_LEFT_4_SEQUENCE=GAACCCCATTTGAAAAAGACAG
PRIMER_RIGHT_4_SEQUENCE=GCCACAAACACTACTCCTTTGA
PRIMER_LEFT_4=245,22
PRIMER_RIGHT_4=387,22
PRIMER_LEFT_4_TM=59.850
PRIMER_RIGHT_4_TM=59.286
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_00914 Ps_contig_mira-and-tgicl-ass_franssen_8758
SEQUENCE=TAAGAGCATATGTGGAGCTGATGGGTATGATGAGATCGGCGGTGGAGACGGGGAGCTTGGCGAACGGGACTGAAACAACGGCGGAAGGTAGGTGGGTGGTGGCGGTGGTGACGGCATGAATTCACAACAACCGTCTAGTGCGTGACCACCTAAAGTTGCCACGTGGTTTTTGAGACATTCTTTGTAAGTAACAGCAACAACGTTGTTGTTGTGGTGGTTGTGAGTGTTGGGATGGTGGCGTTTAAGAACCCCATTTGAAAAAGACAGAGGCTTTGTTATGTTTGAGGTTGTTGTGTTAACGGGTTGTTGTTGTTGGATTCGGGTCGGGTTTTCTGAATCGGGTTCTGATTGGTTTTGAAATTGTTGTCAAAGGAGTAGTGTTTGTGGCGGTTGTTGTTGTTGTTAAAACCTCCATCATGATAAATCTAAAAAAGATTAAAATTAAAAAAACTTACCTTAGTTTCAATTATGTGGTTCTAACTTCTAAGTTTATAAAACCTCATAAATGTATCACATCATACACTATAGTCTCCATGAAGAAGCTAGCTTGGTTTTGAGCTTATTTTTTTCTGAAAATTTGAGGAATTGAAAGGAGAACAAAAAGAAAGAGGAGAAGTGAGTGGTGGAGGAGGAGAAGTGGTGGTGGTTGTGG
TARGET=390,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1490
PRIMER_LEFT_PENALTY=1.058858
PRIMER_RIGHT_PENALTY=0.090155
PRIMER_LEFT_SEQUENCE=TTCTGAATCGGGTTCTGATTG
PRIMER_RIGHT_SEQUENCE=CTCCACCACTCACTTCTCCTCT
PRIMER_LEFT=330,21
PRIMER_RIGHT=627,22
PRIMER_LEFT_TM=60.059
PRIMER_RIGHT_TM=59.910
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=1.2703
PRIMER_LEFT_1_PENALTY=1.180191
PRIMER_RIGHT_1_PENALTY=0.090155
PRIMER_LEFT_1_SEQUENCE=GAATCGGGTTCTGATTGGTTT
PRIMER_RIGHT_1_SEQUENCE=CTCCACCACTCACTTCTCCTCT
PRIMER_LEFT_1=334,21
PRIMER_RIGHT_1=627,22
PRIMER_LEFT_1_TM=60.180
PRIMER_RIGHT_1_TM=59.910
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=1.2805
PRIMER_LEFT_2_PENALTY=1.058858
PRIMER_RIGHT_2_PENALTY=0.221594
PRIMER_LEFT_2_SEQUENCE=TTCTGAATCGGGTTCTGATTG
PRIMER_RIGHT_2_SEQUENCE=CCACCACTCACTTCTCCTCTTT
PRIMER_LEFT_2=330,21
PRIMER_RIGHT_2=625,22
PRIMER_LEFT_2_TM=60.059
PRIMER_RIGHT_2_TM=59.778
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=1.3466
PRIMER_LEFT_3_PENALTY=1.058858
PRIMER_RIGHT_3_PENALTY=0.287765
PRIMER_LEFT_3_SEQUENCE=TTCTGAATCGGGTTCTGATTG
PRIMER_RIGHT_3_SEQUENCE=TCCACCACTCACTTCTCCTCTT
PRIMER_LEFT_3=330,21
PRIMER_RIGHT_3=626,22
PRIMER_LEFT_3_TM=60.059
PRIMER_RIGHT_3_TM=60.288
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=1.4018
PRIMER_LEFT_4_PENALTY=1.180191
PRIMER_RIGHT_4_PENALTY=0.221594
PRIMER_LEFT_4_SEQUENCE=GAATCGGGTTCTGATTGGTTT
PRIMER_RIGHT_4_SEQUENCE=CCACCACTCACTTCTCCTCTTT
PRIMER_LEFT_4=334,21
PRIMER_RIGHT_4=625,22
PRIMER_LEFT_4_TM=60.180
PRIMER_RIGHT_4_TM=59.778
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_00915 Ps_contig_mira-and-tgicl-ass_franssen_8766
SEQUENCE=AYAGAAAAATAAATAAATCAACCAAGCARTCAAACGTACYAGGATCGGTTCATAGTGTGCAAAATCTCATGTTTGAGTACATTCACTAAGACGCGTAATAAGGTGATCTAGGAATTAGCATAAATAAAATACATTTGAGCAATTGGTACTACCCTTCCCCTAATATTAAGCACAAACAATTATTTCTCAATCTATTACAAAACCGTTTAACCAATNTGCCAAATAATAAATGCCGCTAATTAAA
TARGET=7,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00916 Ps_contig_mira-and-tgicl-ass_franssen_8767
SEQUENCE=TATGGAGATCACATGATTGTCGTCTTGCCATCATTTCATGACATTAAGTGATCCAGCATTTTCCTGCCATTGAAGATTTAATTATGCGGCATTTATATTGGCATATTGGTAAACGTTTTGAATAGATTGAGAAATAATTGTTTGTGCTTAAATATTAGGGGAAGGGTATACCAATTGCTCAAAATTGTATTTTTATTATGCTAATTCCTAGATCACCTTATTACGCGTCTTAGTGAATGTACTCAAACATGAGATTTTGCACACTATGAACCGATCCTAGTACGTTTGATTGCTTGGTTGATTTTATTTTATTTTTCTAGTAAAAAGAAAAAGGTGTAGAACTTGTTTTGGGATACTCGGATCATAATCGGTAAACCATGCATTGCTAGAATGTAGTAAAAATT
TARGET=301,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1318
PRIMER_LEFT_PENALTY=0.003166
PRIMER_RIGHT_PENALTY=0.128591
PRIMER_LEFT_SEQUENCE=GATTTTGCACACTATGAACCGA
PRIMER_RIGHT_SEQUENCE=CCGAGTATCCCAAAACAAGTTC
PRIMER_LEFT=252,22
PRIMER_RIGHT=359,22
PRIMER_LEFT_TM=60.003
PRIMER_RIGHT_TM=59.871
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=108
PRIMER_PAIR_PENALTY_1=0.1678
PRIMER_LEFT_1_PENALTY=0.003166
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=GATTTTGCACACTATGAACCGA
PRIMER_RIGHT_1_SEQUENCE=GATCCGAGTATCCCAAAACAAG
PRIMER_LEFT_1=252,22
PRIMER_RIGHT_1=362,22
PRIMER_LEFT_1_TM=60.003
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=0.3025
PRIMER_LEFT_2_PENALTY=0.003166
PRIMER_RIGHT_2_PENALTY=0.299343
PRIMER_LEFT_2_SEQUENCE=GATTTTGCACACTATGAACCGA
PRIMER_RIGHT_2_SEQUENCE=GGTTTACCGATTATGATCCGAG
PRIMER_LEFT_2=252,22
PRIMER_RIGHT_2=376,22
PRIMER_LEFT_2_TM=60.003
PRIMER_RIGHT_2_TM=59.701
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.5652
PRIMER_LEFT_3_PENALTY=0.003166
PRIMER_RIGHT_3_PENALTY=0.562082
PRIMER_LEFT_3_SEQUENCE=GATTTTGCACACTATGAACCGA
PRIMER_RIGHT_3_SEQUENCE=ATGATCCGAGTATCCCAAAACA
PRIMER_LEFT_3=252,22
PRIMER_RIGHT_3=364,22
PRIMER_LEFT_3_TM=60.003
PRIMER_RIGHT_3_TM=60.562
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=0.8175
PRIMER_LEFT_4_PENALTY=0.003166
PRIMER_RIGHT_4_PENALTY=0.814346
PRIMER_LEFT_4_SEQUENCE=GATTTTGCACACTATGAACCGA
PRIMER_RIGHT_4_SEQUENCE=ACCGATTATGATCCGAGTATCC
PRIMER_LEFT_4=252,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=60.003
PRIMER_RIGHT_4_TM=59.186
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_00917 Ps_contig_mira-and-tgicl-ass_franssen_8769
SEQUENCE=CGATTTCCTTTTCTTCTTCATTTTCATCCTCTATAATTCCATTTTCTTCTTCTTCGTTTCTTANGTTTCCAAACCCACGCTCTTACAATCCCTTTCCCAATGCATTCATATGTTAATCCAAAAATCGCTTTTACTATACTGAATTAACTGATTATGCTTAATAATCACTHTTTTCTGAACAGGTGTATCTCAGAAGCTTGATGCTCTCGGTGTTGATTTTGAATTCAAGGTAATTTTATGGGGAATGTACTCGGTCCGAACAGTACCAAGGCTAGAGGAAGTGTTTCAACGAGCGAGGTTTTGCCGGGTGAAGCTTGCAAGCGACAGAGATTGTTGTCGACTTCTGATGAGGATAACTCTAGACTGATTCCTTCTCTTCCTGATGAGATATCGGTTCAGATTCTGGCTTAGGGTACCTCGCGTTTGTTACTTGAATCTAAAGTCGGTTTCTCGCACTTGGAAGATGGCGCTTGGGAGTTCGGATCTGTTTTGTTGAGAAAGGACTTGGAACAACGGAGGAGTGGTTGTAATGTTAA
TARGET=44,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00918 Ps_contig_mira-and-tgicl-ass_franssen_8779
SEQUENCE=TGCATTTTTTATTCAATACAATATTAATCTTACTTCTAAACTTCAAATGCATAATAAACCCTACCAGAAAAGCCAGCTTCATTTTTCTTTATATATATAGACCACCACTCTCTTCCACTTTTAAGTATTAAAAAAACCACAAGAGTTGGATGCTCACACTTTAAGTCTTCTTCGTTTTTCATCCGTATTTTCCACTCACTTTTTTTCTTTTCACTTTTTCTTCTTCCTGTTACRCCCTWCTKGTAATGATAGCTTTCAACACCCGTGAACCACAAACCCAAATATCTTCACCAAAACTTGGTTTCCCTCACTATAGATATTTCAGATACACTTTCCCTTGCAATCCCCGATGCCAAACAACAAAAACCAACGTAACAGTCCGGTGGAAACTCACCGGCGAGGCAAACTAACAGAGAAATCATCGTYYTTTTACGGTTCACACTACCTCCACGGCAGCYACGGTTCGGTACCGAA
TARGET=90,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00919 Ps_contig_mira-and-tgicl-ass_franssen_8798
SEQUENCE=CTAGGAGATTGATGATATTGTGTATTTGTTGATGAAGCCAAAACTCCCGCTCTAATATCATTGAGAGCCTCAGCTGAAGCATTCCAGAAAGGAGTATTGTTTGAAAAGTGCAATCCACTAAGCTGTTGCTTTGGCATTGAAGATGAATAGGACATAGAAGAAGGGTTGGTAAAAAGTGAGTTTTGTGGCTGTGGTTGAGAATCAAATAAACTTTGTATCAAACTTGAAGGGTAACAACCATAAGAATCAACTGATCCAATTGGATATGATGATAAACTTTGATGATCCATGACAAAAATTACTWGTTCCATTGAAGATGAGAAATTCTTTGTGCTTTCTTCTTGAATCACAGGCTCAAAATTGTTCTCTGACAATATTTGAGCGATTCCAATTTGATGAATTTGGTAGTATTGTTAGTTAGTTATTTCAAAGAACATGCTACTAGTACTACCGGTTCCGTTAACCGATATTTCCGGCGGCTT
TARGET=413,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00920 Ps_contig_mira-and-tgicl-ass_franssen_8801
SEQUENCE=GAAATAAAAAGAAGAACAAGAAAGAACTCTAGAAGTAAGAAGAAAASATTTTCATTTTAATACCAACAGCAACAACGAGTAAGCGCATTTCTCGTGACTCTATTTATTCTGTCTTTGCGTTTGGGTGTAAATCACCTAAGACGCGTTCTACAGCTACACACTCTGAGTCTGTGTGAATGGGTTCTGACGGAGCAACTTCGGGCGGCGAACAGGTCGGAAGGAAACCGTGTGGAGGGAGAGAGAGAAA
TARGET=236,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00921 Ps_contig_mira-and-tgicl-ass_franssen_8824
SEQUENCE=AGCCATTTTGTGATTTTTTCTCTTTCCGTTATTGATTCAATGAAACTCAAAAATGGGTTACTRTTGTTCTTTATGTTTCTGGATTGTATTTTTTTCAAAGTGGAATCCAAGTGTGTAATAGGGTGTGATATAGCTTTAGCTTCCTACTATGTAATGCCTTTAGTTGAACTCTYAAATATAACAACCTTTATGCAATCAAARCTTSTTACCAATTCTTCTGAGGTTATAGTAAGGTACAACAGAGACATTGTATTCAGTAATGATAATCTTTTTTCCTATTTTAGAATCAACATTCCATTCCCATGTGAATGTATTGGAGGTGARTTTCTKGGGCATGTGTTTGAATAYACAGCAAATGAAGGAGATACTTATGATTTAATTGCAAATACCTATTATGCAAGCTTAACAACTGTTGAGGTTTTGAAAAAGTTCAACAGCTATGATCCAAATCATATACCTGCTAAGGCTAAGGTTAATGTCACTGTTAATTGTTCTTGTGGGAACAGTCAGATTTCAAAAGACTATGGGCTGTTTATCACTTATCCACTTAGGCCTAGGGATACTCTTGAGAAGATTGCAAGCCATTCTAAACTTGATGAAGGGGTGATACAAAGTTACAACTTGGGTGTCAATTTCAGCAAAGGCAGCGGGATAGTGTTCTTTCCGGGAAGAGGTATGYATGTTTTTCTTCTTTTCCACCACCTTTTGATCTTCAGATCAAAAGTATTTGGTAKAAATATATCTGTTMKAGTATATTCAATGAATAAATTAYTCTACCAACTGTGGTKGGCAMAAATGGTCGGRCATTCCGCTCACMCCCGCGGTGACTTGGGTAAATATGAAATAATGMAGTTGGTAGGTTATACCTAAACTGCATTGTTAATCCTCAGTTGGTGGTTCTGATCAAAAGTACYTGGTATAAATATGAGTTAGATAATTTTTTATGGTCTCTATCCAGTAAGTGTTCCAGTTAACATTTGAATTCAGGTACTTCGGGTCAATTCAACCTCATATAAACTAATTAGTCACTAGAGTTCAACCATTTGGTTTCAATTATAGTATATTCAATGGATAACTTACTCTGTTATTCTTCTTCAAAGTATATATATGGTATTTATGGTAATGACTTTGCTTAAAGTTTATTTATTTTTATCTTTGCAGATAAAAATGGAGAATATGTTCCTTTATATCCTAGGTAGGTAGCTTTGATTATGTCACTTATCATGTTGAATGCTATAGAAGAAGTAAATATTGCTTCATATTTGTTTCTATTCCTTCTAACTTTCATTTTTTCCCTTTTGCAGAACAGGTTTGGCATTCTGACTTTGTATTATGATGATTAATTTTAGCTTGATCCTGTTCCAAAAATAAAACAGAGAATGCAATAATGGTCGGTCATTGCGTCTTGTGAGATTAAATTTGGTTCTATATAAGCAGGTCTTGGTAAGGGTGCAGCTGCTGGTATATCTATAGCTGGAATATTTGCACTTCTGTTATTTGTTATCTGCATATATATCAAATACTTCCAAAAGAAGGAAGAAGAGAAAACTAAACTGCCACAAGTTTCTACGGCGCTTTCAGCTCAAGATGGTAATGGTATATTTTTGTATTCATATTCTAATTGAAGTTCTTATTAAACTATTTATTTGAACTTATCTTATGGTATAAGCACTTGTATAGTATTTGAGAAATCTTACAAAATATAGTTTACGACATATTCGGAAGTTGTTCATTTATTTCAATAAGCTTTCGGTGATAGTTTATGGAAACAACTTACTACTTATATGAAAACAGTTTAATCATATTTCCACTTTGATTATGAAAATAGACTATGGTTGTTGATTTTTGTTTCTGTTCCCGGTGAAGCCTCGGGTAGTGGAGAGTACGAAACTTCGGGATCCAGTGGGCATGGWACCGGTAAGTAACTAGACTAGGCCTTACAGGAATTATGGTGGCAAAGTCAACTGAGTTTTCATATCAAGAACTAGCCAAGGCTACAAATAACTTCAGCTTAGATAATAAAATTGGTCAAGGTGGATTTGGAGCTGTCTATTATGCAGTACTCAGAGGCGAGGTGTGTAACTGCGTCTATATCTTTATAAAACTAAAAATGAATAATATTAGCTTGTTTGATCGTGTTTTGTTTTGAAAATGTTTTATAAAACTTTATTTTTGAAATTGGGGAAGAAAACTATTATTGAAGTTGGTCTAATGACCATTTTACAATTATTTATGAGGGAATTTGAACTGTATTTCTTGAAGTTACCCGGTCAAACTCTTATTACTGAGTTGATATCTCATGTACATACAATACTTTTTTCCTTAACAAGAAATTAATAAAAGATTTTGTAAATTGTGTAGCACCGTTTACTTAAGGGTGAACTGGAAAGTGTTTGTTTCTCTCTCTTCACATAGAATAACCACTTTTTTCACTCTTTTCAAGTTGAATTTCCTCTCATACAATTTCTTCTATGCAGAAAACAGCAATTAAGAAGATGGATGTACAAGCGTCRACAGAATTYCTTTGCGAGTTGCAAGTCTTAACACATAGTTCATCACTTGAATCTGGTGAKAATATCCTTCAACATTTTAAGCCATATATGATGTACTCCTACTTGGGAAGGATAGCCAATCATTTTTATGCATGGCTTGAAAATAATCATTTAAAACAAAGCAAGTATAACTAATGTGATTTTGAAAACAGAACAGTTCTATGACTATCAAGCAAAATACATGCAAAACATTTAATATGTAACTTGTTGACCATTATCTGTCCTATAAAGTTAAAAAATATTTAAGACACTGCATAACATTATTTGATTTTGAATATATTAGTTACATGTTTGCATACTTTTAATTTATTTGTTTTTCCATATTTGAAATATTTACCTTGCATTTGCATAAAATGTTTCAAGTATGTTATAAATCTTTTTCTTAATTTACCTATATTTTTGTGAACAACATAGGTGAGGTTGATTGGATATTGTGTCGAGGGATCACTTTTCCTTGTATATGAACATATTGACAATGGAAACTTGGGTCAATATTTGCACGGTATAGGTAAGATCAGTGACAGAAAGTGCTTTTTTGTGATCTTACAATATTGTCAAACATTTATTAACAATGGTGATATGACTCCAGATAAAGCGCCATTACCATGGTCAAGTAGGGTGCAAATTGCTCTAGATTCSGGTCAAGAGGCCTTGAATACATTCATGAACACACTGTACCTGTGTATATCCATCGTGATGTAAAATCAGCAAATATATTAATAGACAAAAACTTGCACGGAAAGGTTGCAGTATAACCAATCTAAACAATCTTATTTCCAAATTTTCCTTTATCTGTTGAGATTAATTAAACTATGAATGGTCTTAAACTCAGGTTGCAGATTTTGGCTTGACCAAACTTATTGAAGTTGGAAATTCCACACTCCACACTCGTCTCGTGGGAACATTTGGATACATGCCACCAGAGTATGATTTATTTTTAATTTGTATTAAATTCGAGTGCATCCTATGTCACTTTATTATATTACATAGTGAGATAACAATGAAGAGCTTAGTAACAAAAGGTTTTTGTATCATTGTATGATTAATTAGTAAATCAAACCTCTTGGTTTATTAACTAACTAAATTGTYYWSMAGAATATGCTCAATATGGCGATGTTTCTCCAAAAATAGATGTATATGCTTTTGGAGTTGTTCTTTATGAGCTTATTTCTGCAAAGAATGCTATTCTGAAGACAGGTGAATCTGCTGTCGAATCAAAGGGTCTTGTAGCATTGGTATGTCTAAAATCAAGATACCCTTTTATCCCACTATAGCATTTTTACTTATAAAACACTCGATTAAATTTCATAGTCAAATGTTAGTAAGCTAGTTGCAATGAGGTTATCCTTGACTTGTCTTGTATTGCAGTTTGAAGAAGCACTTAATCAGATCGATCCTTTAGAAGCTCTTCGCAAATTGGTGGATCCTAGGCTTAAAGAAAACTATCCAATTGATTCTGTTTTAAAGGTTGGAATCATTTCTCTTAGAAGAAAATTTGAATGTTATGATGTGATAAAGTTGTGGAATATAATTGAATCTATGTATGTAAAAGTTATTTTCGAAATGAGTGAGAGTTACTACGCCCAAAAATCTTGGTACCAGATTTTCTTAATGTGTGGTGGATGTTTGAATTGCAKATGGCTCAACTTGGGAGGAGCATGTACAAGAGACAATCCACTACTACGCCCAACGTATGAGATCTTTAGTCGTTGCTCTTATGACACTCTTATCACATACTGATGATGATGACACTTTCTATGAAAATCAATCTCTCACAAATCTATTATCAGTGAGATGAAGGCTTTGTGTGCCAAATTGAATGATGTTTGTGAAAACTTTTAGAAGCATACAGCAAAATGTTTGTACTCTGAACATAATATTGAGGTTAGGAAGTTTTGATCTTGTGTTCAAATTTTATTTCCCAAAATAGTCAAAAAGTCCTAGATCCAAAGAAGACATCCTGTAATTATTTTTAGTGACGCTGTAANACTAAAGTACAG
TARGET=4290,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00922 Ps_contig_mira-and-tgicl-ass_franssen_8825
SEQUENCE=ATTTTCAAGAAATGAATTTTGTACTACATATTAATCATTCGTTGCTGATTTGAGTTAATTTCTTTTTCTGCTTCTTGCTTTCCTTCGGCAGCCATTTTGTGATTTTTTCTCTTTCCCTTATTGATTCAATGAAACTCAAAAATGGGTTACTGYTGTTCTTTATGTTTCTGGATTGTATTTTTTTCAAAGTGGAATCCAAGTGTGTAATAGGGTGTGATATAGCTTTAGCTTCCTACTATGTAATGCCTTTAGTTCAACTCTCCAATATAACAACCTTTATGCAATCAAAGCTTGTTACCAATTCTTTTGAGGTTATAGTAAGGTACAACAGAGACATTGTRTTCAGTAATGATAATCTTTTTTCCTATTTTAGAGTCAACATTCCATTCCCATGTGAATGTATTGGAGGTGAATTTCTTGGGCATGTGTTTGAATACACTGCAAATGAAGGYGATACTTATGATTTAATTGCAAATACCTATTATGCAAGCTTAACAACTGTTGAGGTTTTGAAAAAGTACAACAGCTATGATCCAAATCATATACCTGTCAAAGCTAAGGTTAATGTCACTGTTAATTGTTCTTGTGGGAACAGCCAGATTTCAAAAGACTATGGGCTATTTATCACCTATCCACTTAGGCCTAGGGATACTCTTGAGAAGATTGCAAGACATTCTAAWCTTGATGAAGGAGTAATACAAAGTTACAATTTGGGTGTCAATTTCAGCAAAGGCAGCGGGGTAGTGTTCTTTCCCGGAAGAGGTRTGTATGTTTTTCTTCATTTCCACCACCCTTTGATGTACAGATCARAAGTATTGGGTATAAATATATCTGTTAGAGTATATTCAATGAATAAATTACTCTACCAACTGCGATTGGCACAAATGGTTGGGCATTCCAMTCTCGCCCCCGGTGAATCGGGTAAATATGAAATTATGCAGTTGGTAGGTTATAYGTAAACTCCATTGTTAATCCCAATGAAATTGGTGGTTCCGATCAGAAGTACTTGGTATAAATATGAGTTAGATAATTTATTATGGTCCTCTATCCAACAATTGTTCTAGTTAGCGTTTGAATTAAGGTACTCCTGGTCAATTCAACCTCATATAAACTAATTAATCACTAGAGTTCAACCACTTGGTTTCAATTGTAGTATATTCAATGGATAACTTACTCTGTTATTCTTCTTCAAAGTATATATATGGTATTTATGGTAATGACTTTGCTTAAAGTTTATTTATTTTTATCTTTGCAGATAAAAATGGAGAATATGTTCCTCTATATCCTAGGTAGGTAGCTTTGATTATGTCACTTATTATGTTGAATGCTATAGAAGAAGTAAATATTGCTTCATATTTGTTTCTATTCCTTCTAACTTTCATTTTTTCCCTTTTGCAGAACAGGTTTGGCATTCTGACTTTGTATTATGATGATTAATTTTAGCTTGATCCTTGTTCCAAAAATAAAACAGAGAATGCAATAATGGTCGGTCATTGCTTCTTGTGAGATTAAATTTGGTTCTATATAAGCAGGTCTTGGTAAGGGTGCAGCTGCTGGTATATCTATAGCTGGAATATTTGCGCTTCTGTTATTTGTTATCTGCATATATATCAAATACTTCCAAAAGAAGGAAGAAGAGAAAACTAAACTGCCACAAGTTTCTACGGCGCTTTCAGCTCAAGATGGTAATGGTATATTTTTGTATTCATATTCTAATTGAAGTTCTTATTAAACTATTTATTTGAACTTATCTTATGGTATAAGCACTTGTATAGTATTTGAGAAATCTTACAAAAAATAGTTTACGACATATTCGGAAGTTGTTCATTTATTTCAATAAGCTTTCGGTGATAGTTTATGGAAACAACTTACTACTTATATGAAAACAGTTTAATCATATTTCCACTTTGATTATGAAAATAGACTATGGTTGTTGATTTTTGTTTCTGTTCCCGGTGAAGCCTCAGGTAGTGGAGAGTACGAAACTTCGGGATCCAGTGGGCATGGTACCGGTAGTACTGCTGGCCTTACAGGCATTATGGTGGCAAAGTCAACTGAGTTTTCATATCAAGAACTAGCCAAGGCTACAAATAACTTCAGCTTAGATAATAAAATTGGTCAAGGTGGATTTGGAGCTGTCTATTATGCAGTACTCAGAGGCGAGGTATGTAACTGCATCTATATCTTTTATAAAACTAAAAATGGATAATATTAGCTTGTTTGATCGTGTTTTGTTTTGGAGATGTTTTATAAAACTTTATTTTTGAAATTGGGGAAGAAAACTATTATTGAAGTTGGTCTAGTGACCATTTTACAATTATTTATGAGGGAATTTGAACTGTATTTCTTGAAGTTACCCGGTCAAACTCTTATTACTGAGTTGATATCTCATGTACATACAATACTTTTTTCCTTAACAAGAAATTAATAAAAGATTTTGTAAATTGTGTAGCACTGTTTACTTAAGGGTGAACTGGAAAGTGTTTGTTTCTCTCTCTTCACATAGAATAACCACTTTTTTCACTCTTTTTAAGTTGAATTTCCTCTCATACAATTTCTTCTATGCAGAAAACAGCAATTAAGAAGATGGATGTACAAGCGTCGACAGAATTTCTTTGCGAGTTGCAAGTCTTAACACATGTTCATCACTTGAATCTGGTATAATATCCTTCAACATTTTAAGCCATATATGATGTACTCCTACTTGGGAAGGATAGCCAATCATTTTTATGCATGGTTTGAAAATAATCATTTAACAACAAAGCAAATATAACTAATGTGATTTTGAGAACAGAACAGTTCTATGACTATCAAGCAAAATCATTTCAGCAATGCAAAATATAACTAAAAAAACATTTAATATGTAACTTCTTGACCATTATTGGTCCTATAAAGTTAAAAAATATTTAAGACACTGCATAACATTATTTGATTTTGAATATATTAGTTACATGTTTGCATACTTTTAATTTATTTGTTTTTCCATATTTGAAATCTTTACCTTGCATATGCATAAAAAGTTTCAAGTATGTTATAAGTCTTTTTCTGAATTTACCTATATTTTTATGAACAACATAGGTGAGGTTGATTGGATATTGTGTCGAGGGATCACTTTTCCTTGTATATGAACATATTGACAATGGAAACTTGGGTCAATATTTGCACGGTATAGGTAAGATCAGCGACAGAAAGTGCTCTTTCGTGGTCTTACAATATTGTCAAACATTTATTAACAATGGTGATATGATTCCAGATAAAGCGCCATTACCATGGTCAAGTAGGGTGCAAATTGCTCTAGATTCCGCAAGAGGCCTTGAATACATTCATGAACACACTGTACCTGTGTATATCCATCGTGATGTAAAATCAGCAAATATATTAATAGACAAAAACTTGCACGGAAAGGTTGCAGTATAACCAATCTAAACAATCTTATTTCCAAATTTTCCTTTATCCGTTGAGATTAATTAAACTATGAATGGTCTTAAACTCAGGTTGCAGATTTTGGCTTGACCAAACTTATTGAAGTTGGAAACTCCACACTTCACACTCGTCTAGTGGGAACATTCGGATACATGCCACCAGAGTATGATTTGTTTTTAATTTGTATTAAATTCGAGTGCGTCTTATGTCACTTTATTATATAGTGATATGACGATGAAGAGCTAGTTACAAAAGGTTTTTGTATCACTGTATGATTAATTAGTAAAATCAAACCTCTTGGTTTATTAACCAACTAAATTGTTTTGAAGATATGCTCAATATGGCGATGTTTCTCCAAAAATAGATGTATATGCTTTTGGAGTTGTTCTTTATGAGCTTATTTCTGCAAAGAATGCTATTCTGAAGACAGGTGAATCTGCTGTCGAATCAAAGGGTCTTGTAGCATTGGTATGTCTAAAATCAAGATACCCTTTTATCCCACTATAGCATCTTTACTTACAAAACACTTGATTAAATTTCTTAGTCAAATGTTAGTAAGCTAGTTGCAATGAGGTTATCCTTGACTTGTTTTGTATTGCAGTTTGAAGAAGCACTTAATCAGATCGATCCTTTAGAAGCTCTTCGCAAATTGGTGGATCCTAGGCTTAAAGAAAACTATCCAATTGATTCTGTTTTAAAGGTTGGAATCATTTCTCTTAGAAGAAAATTTGAATGTTATGATGTGATAAAGTTGTGGAATATAATTAAATCTATATATGTAAAAGTTAGAGTTACTATGCCCAAAACTCTTGGTACCAGATTTTCTTAACGTGTGGTGAATGTTTGAATTGCAGATGGCTCAACTTGGGAGAGCATGTACAAGAGACAATCCACTACTACGCCCAAGTATGAGATCTTTAGTCGTTGCTCTTATGACACTCTTATCACATACTGATGATGATGACACGTTCTATGAAAATCAATCTCTCACAAATCTATTATCAGTGAGATGAAGGCTTTATGTGCCAAATTGAATGATGTTTGTGAAAACTTTTAGAAGCATACAGCAAAATGTTTGTACTCTGAACATAATATTGAGGTTAGGAAGTTTTGATCTTGTGTTCAAATTTTATTTCCCAAAATAGTCAAAAAGTCCTAGATCCAAAGAAGACA
TARGET=4376,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00923 Ps_contig_mira-and-tgicl-ass_franssen_8842
SEQUENCE=ACTTCNCACNGCACGAGTNTTCTTCTTTCTTCTTNTTTCCTTCTTCTTCTTTCTTTTCTCAMCCTCAATTCCGCACTCACTTTCCTCTTCTTATTGAATTGCAACCTTCAAACATGGCGTAACATCTCTCTTTCGTTCTCTGATTCACTTCACTCTTCACCACCTTCTTTCACTACTACTCTTGTCTCTTCTGGTGGTCTTTTCTGTTTAACCGATTCTCTTTCATGTTCTTTACTTGTTTGTAACCTTTTAGCCAAATCCAAAAAGAAAAATTCAATACCCTAATTTCAATCTTCATATTGAGCATCTTACCTTTGTTTCAACTCCAAAAGGGTACTTCATTTTTATACTCTCTTCTGAATCATCTTCAAATAA
TARGET=40,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00924 Ps_contig_mira-and-tgicl-ass_franssen_8858
SEQUENCE=ACTGAGTTGACCCAAATAATTATCAACCCCTTAGCTCAACAGAAGCACCTAACAAGATTGGAATGAGACAGGGCAAAACCGAAACCTATACATTTTAGAATCATAACATATTGAAAACAACAACAACACTCTTAAACCAAA
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00925 Ps_contig_mira-and-tgicl-ass_franssen_8865
SEQUENCE=CGAAAAATACTAGTTTGCTTCTTTCTGTTTACGGTTAGTTACCAATCACTAATCTCTATCCACACCACCATTGTTGCTATTTGCAGTGTAACCCTTTTTTCTCGCTTCCACTGCTACTCTCGCTTTTTCTCTTTCCAAGCTCACTATCCATCCCAACAGTTGAGTTAGATCTTCCTCTTCTTCCGCAACATTGCGCCACCATGAAAGAAACCACCACTAATAATGCCACGGAAACTGCCACTGTAACCGCCACAATGGCACCGGGACTCAACATTTTCCGTTTGCTCGAAGGAGTTTCAGATGGAATGATACCGTTAGCATTTCTACTTGATTTTGGCATAGAGCTAGGGTTTGAAGGAACAATTTCAGAACCATCATCAGAAGGAGGAGGAGGATGAGTTTCGGTGACAGGACAATTTGGTAACGGCGTCAATCCGCATAGATCATCGTTGCCGGAGAAGCTTTTGTTGCCGAATTTGGTAAGCATGGCGTCTGGTAAGCGTCCTTGAAGTTCGTTGTTAGTAATGTTGAGTTCTCTGAGATTGAGTAGAGAAGAAGAAAGATCCGGAACTTCACCGGAGAGTTCGTTGTTCTGTAACCGGAGAGTGAGGAGATATTTCAGTTTC
TARGET=383,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2221
PRIMER_LEFT_PENALTY=0.129103
PRIMER_RIGHT_PENALTY=0.093026
PRIMER_LEFT_SEQUENCE=GCTTTTTCTCTTTCCAAGCTCA
PRIMER_RIGHT_SEQUENCE=AAATTGTCCTGTCACCGAAACT
PRIMER_LEFT=121,22
PRIMER_RIGHT=418,22
PRIMER_LEFT_TM=60.129
PRIMER_RIGHT_TM=59.907
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=0.2491
PRIMER_LEFT_1_PENALTY=0.074466
PRIMER_RIGHT_1_PENALTY=0.174657
PRIMER_LEFT_1_SEQUENCE=TCGAAGGAGTTTCAGATGGAAT
PRIMER_RIGHT_1_SEQUENCE=AACGAACTTCAAGGACGCTTAC
PRIMER_LEFT_1=285,22
PRIMER_RIGHT_1=516,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.825
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.2581
PRIMER_LEFT_2_PENALTY=0.074466
PRIMER_RIGHT_2_PENALTY=0.183585
PRIMER_LEFT_2_SEQUENCE=TCGAAGGAGTTTCAGATGGAAT
PRIMER_RIGHT_2_SEQUENCE=ACTTCAAGGACGCTTACCAGAC
PRIMER_LEFT_2=285,22
PRIMER_RIGHT_2=511,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.816
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=0.2812
PRIMER_LEFT_3_PENALTY=0.149516
PRIMER_RIGHT_3_PENALTY=0.131699
PRIMER_LEFT_3_SEQUENCE=GGGTTTGAAGGAACAATTTCAG
PRIMER_RIGHT_3_SEQUENCE=ATCTCCTCACTCTCCGGTTACA
PRIMER_LEFT_3=347,22
PRIMER_RIGHT_3=614,22
PRIMER_LEFT_3_TM=59.850
PRIMER_RIGHT_3_TM=60.132
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.3166
PRIMER_LEFT_4_PENALTY=0.149516
PRIMER_RIGHT_4_PENALTY=0.167063
PRIMER_LEFT_4_SEQUENCE=GGGTTTGAAGGAACAATTTCAG
PRIMER_RIGHT_4_SEQUENCE=CCTCACTCTCCGGTTACAGAAC
PRIMER_LEFT_4=347,22
PRIMER_RIGHT_4=610,22
PRIMER_LEFT_4_TM=59.850
PRIMER_RIGHT_4_TM=60.167
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_00926 Ps_contig_mira-and-tgicl-ass_franssen_8869
SEQUENCE=CAACGCAGAGTASTCGGGGGACGCGAGCCACTCACACACTGGGGGTTCCCCCAACAACACGCGCCCTTCCCCGGCGACCGTACTGTATTATCCCATTCTTTCGCCTTTGTTTTCCTTCCGCCGTTTCTCTTTCTATATCTCTATCTCTTTCTCTCTCATCATTCCTCAACCCTAATTTCTCTTTTTTCACCGATGCCGGACTTTCACGATTCTCTCTCTCCGCCGTCACGTCCTGTCCCTGTCCGTGAGGATTGCTGGTCTGAGGAAGCTTCTTCCACTCTGGTAGATGCTTGGGGTCGGCGTTACCTTGAACTCAACCGTGGTAATCTTCGTCAGAAGGATTGGCAGGATGTTGCCGATGCCGTTAACGCTCTTCATGGTCATACGAAGAAAACTCATCGCACCGATGTTCAGTGTAAGAATCGAATCGATACGATTAAGAAGAAGTATAAGATCGAGAAAGCTAGGGTTTCGTCTTCCAGTGGTGTTGTTTCTTCGTCTTGGCCTTTTTTCGAACGGTTAGATGCTTTGGTTGGACCTAATTTCAATTCGAAGAAAGCTTCCTCTTCGCCATCGCCGTCGCCGCCGATTGCTCTTGCTTTGGTTCCTCATCGGAAATTTCCTTCTTCGTCACCTGCTATTTCTGTGCCTCCGACTGCCGTCGCGCTTCCGCAGAAACGGCCGCTTGCAGCGGTGATGGATGATGGTTATTTCAGGAGGAATTATTCAGCGGTAGCCGCTGCAGCGGCTGCCGCAGAGGCTGATGAAGACGAGGAGGAAGATGAAGAAGAGGAAGTTGAGGAGGAAGAGGTGGAGGTAGAGGAAGAAGAAGGGAGGGGAAGCGAAGTCGATGAAGGGGATAAAGGGAGGGAAGGGATGAAGAGGTTGGCGAAGGCTATAGAGAGTATTTGGTGAAATGTATGAGNGTGGAGGACAAGAAACTGGAGGCAGNGTGGTGGTATTTGGAAGAAAACAAAGGATG
TARGET=211,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00927 Ps_contig_mira-and-tgicl-ass_franssen_8901
SEQUENCE=TACCCAACTGAAAGAAGACCTTCCAATAATCTCAAGAAATAAGCACAAGCGAGACAGTTAGTCCCATGCAAATTTCATCACAACGACATCATACATTTGAAAATGATCAGAAGTCCGAGCATTCAAAGTGCTCAAAAGGGCCAGAAAATCCAGTTGCAGTTGATGACCAAGAGAATCTATTGCAAACTCCTCAATCTCATTTTAAAGATGTTCAACTCAAATCCCAAAATGGTTCTGCTCGAAGTTGCACAAAAGTTCACAAGAAAGGTATCGCTCTTGGTAGGTCGGTTGATCTTACAAAGTTTAACGACTATGATGAACTAGTCACTGAATTGGATCAACTATTTGAATTCGGAGGCGAATTGGTTTCTCCACAAAAGGACTGGCTTGTTGTCTACACTGATAATGAGGGTGACATGATGCTTGTTGGTGATGATCCATGGCAGGAGTTTTGCGCCATGGTACGCAAGATTTACATCTACCCAAAAGAGGAGATTCAGAAAATGAGCCCCGGTACATTGAGTTCTAAAAATGAAGAGAATCATTCAGCTACCGAAGGCGGAGGAGAAGCACAAGAAACAAAATGTCAGATGAATCAAACAACTTCAGATGCTTGATTTCATTCTTTAGTAGATTTAATTTTCTTAGTATCATCCAGATTCCGGACTTTGGAATGGAAAGAAGTAGAGAGGACTGTCATAAAGACAAGCAGAAGCAGTTAGAGAAAAAGTGTATGACCTGAACAAGAAACTTTTAATTCTGAAGAGACAAGAACAACATCAACAACGTATGTGTTTGGAATCAGAATATGGAGTTAGAGTTTTCCATCCGATTTTGGACCATGTCGTGGAATTAGTGTTAGTAGTATATTTATATATATGACTTTCCAATTATAGTATATATAAATAGTATAGCTTGCATTTTTCATCAAATCCTAAACCTTGTTCATAGTTAAGCTAGAATCCATTAATGTAATTATTATTATTATTTTTATTAAGTAGTAGTAGTTCATAATCATATACAAGGGAGGTTTATCTGATTTTTATTTTTAATTAGTATGTGGAAATTAATTATTATGGATTCAATTTGGTATTTTGTCTTGCTTAATGTATATAATTAGTTTAATTATTTAATAKTA
TARGET=975,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00928 Ps_contig_mira-and-tgicl-ass_franssen_8901
SEQUENCE=TACCCAACTGAAAGAAGACCTTCCAATAATCTCAAGAAATAAGCACAAGCGAGACAGTTAGTCCCATGCAAATTTCATCACAACGACATCATACATTTGAAAATGATCAGAAGTCCGAGCATTCAAAGTGCTCAAAAGGGCCAGAAAATCCAGTTGCAGTTGATGACCAAGAGAATCTATTGCAAACTCCTCAATCTCATTTTAAAGATGTTCAACTCAAATCCCAAAATGGTTCTGCTCGAAGTTGCACAAAAGTTCACAAGAAAGGTATCGCTCTTGGTAGGTCGGTTGATCTTACAAAGTTTAACGACTATGATGAACTAGTCACTGAATTGGATCAACTATTTGAATTCGGAGGCGAATTGGTTTCTCCACAAAAGGACTGGCTTGTTGTCTACACTGATAATGAGGGTGACATGATGCTTGTTGGTGATGATCCATGGCAGGAGTTTTGCGCCATGGTACGCAAGATTTACATCTACCCAAAAGAGGAGATTCAGAAAATGAGCCCCGGTACATTGAGTTCTAAAAATGAAGAGAATCATTCAGCTACCGAAGGCGGAGGAGAAGCACAAGAAACAAAATGTCAGATGAATCAAACAACTTCAGATGCTTGATTTCATTCTTTAGTAGATTTAATTTTCTTAGTATCATCCAGATTCCGGACTTTGGAATGGAAAGAAGTAGAGAGGACTGTCATAAAGACAAGCAGAAGCAGTTAGAGAAAAAGTGTATGACCTGAACAAGAAACTTTTAATTCTGAAGAGACAAGAACAACATCAACAACGTATGTGTTTGGAATCAGAATATGGAGTTAGAGTTTTCCATCCGATTTTGGACCATGTCGTGGAATTAGTGTTAGTAGTATATTTATATATATGACTTTCCAATTATAGTATATATAAATAGTATAGCTTGCATTTTTCATCAAATCCTAAACCTTGTTCATAGTTAAGCTAGAATCCATTAATGTAATTATTATTATTATTTTTATTAAGTAGTAGTAGTTCATAATCATATACAAGGGAGGTTTATCTGATTTTTATTTTTAATTAGTATGTGGAAATTAATTATTATGGATTCAATTTGGTATTTTGTCTTGCTTAATGTATATAATTAGTTTAATTATTTAATAKTA
TARGET=997,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00929 Ps_contig_mira-and-tgicl-ass_franssen_8913
SEQUENCE=TCTAGCTTCTCTCCTTCACTTTGAACATTAATTACATCATCAAAACAAATGTATCTACCATAAGCATTTCCGTTCATTTCCTTGAACACACATTCATGAGCTTCAGCTACTTTTTTTACATCAATTGTTGCTAGTAAACCATTTGCATACATTTCTTGTGATCCTTTGAGATAAGCAATTGTTGATGTTGGATTCCTAGGACAAAATTCAGGTCCTGTGATAAGTGCTGGACAAATGGTAGTGAGTTTTAAACCTTTCTCTTTGGCTATTCTCCAAGCTGCCTTCTCTGCCCTCATCTTGCCTAATGCATACCATAGCTTTTTGTTTACGCAGAGTGACTCATTGCTCCAAGATTCATGATTAATAATTGGAGTAAGATTTGAGTTTACATTTTCTTGCCAAATACAAGCAGCTAGTGATGATGTGAATACACATCTTTTAATTGAAGGTGTTCTTGCACATGCTTCCATCACATTTTCTGCTGCATTCGCTTCAATCTCAGCCATGGATTTAGTGTATCCAGAGAGACCAGCGGGGTCAGTAAATGCAGAGGTATGAAAAACGCCACGACATCCTTCAAATGCTTTCACCAAACTATCAACATCACTTAGCTTTGCCATTATTATTTCCAAGTTACTTTCTTCATATCTTTCCATTTCCCTCAATTTCTCTATCTCATCTGGGTTGTCAATAGTTATTCTAACAGAATAACCTAAGACCAAAAGATGGTTGACCAAAGCAAGACCCAAATAAGAAACACCACTTGTAACACAAACCAAGGTTGTTCTTTTTTCTTCATCATCTTCATCTAAAGAAGAAAAATATTTGTTTTTTCTTAACCCTTCATCGTCTTTTCTCCGGTGGATACCGGCGGTAGCCACTAGATTCCGGTGAAAGGCAATAAGCTCTGATCTCTTGGTTTCACATGTGCCTACAACACCCTATTTTTTTTTATATGATATGATATGGTATTTAGTTAGGGATATGAAAAGAATGGATTGTGAAAGGTTTATATTATGGTTATGAAGGTTSAAAGGGGTTNGAAAGGAATATTTATATAATAAAAAAAGNGTGAGTGTGTTGTGTTGTATTGTGTTGTACCTAAGTTCAATNTGGGTCAAGTGAATGAACTATTCATTCATTCCCGAGTTACT
TARGET=954,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00930 Ps_contig_mira-and-tgicl-ass_franssen_8913
SEQUENCE=TCTAGCTTCTCTCCTTCACTTTGAACATTAATTACATCATCAAAACAAATGTATCTACCATAAGCATTTCCGTTCATTTCCTTGAACACACATTCATGAGCTTCAGCTACTTTTTTTACATCAATTGTTGCTAGTAAACCATTTGCATACATTTCTTGTGATCCTTTGAGATAAGCAATTGTTGATGTTGGATTCCTAGGACAAAATTCAGGTCCTGTGATAAGTGCTGGACAAATGGTAGTGAGTTTTAAACCTTTCTCTTTGGCTATTCTCCAAGCTGCCTTCTCTGCCCTCATCTTGCCTAATGCATACCATAGCTTTTTGTTTACGCAGAGTGACTCATTGCTCCAAGATTCATGATTAATAATTGGAGTAAGATTTGAGTTTACATTTTCTTGCCAAATACAAGCAGCTAGTGATGATGTGAATACACATCTTTTAATTGAAGGTGTTCTTGCACATGCTTCCATCACATTTTCTGCTGCATTCGCTTCAATCTCAGCCATGGATTTAGTGTATCCAGAGAGACCAGCGGGGTCAGTAAATGCAGAGGTATGAAAAACGCCACGACATCCTTCAAATGCTTTCACCAAACTATCAACATCACTTAGCTTTGCCATTATTATTTCCAAGTTACTTTCTTCATATCTTTCCATTTCCCTCAATTTCTCTATCTCATCTGGGTTGTCAATAGTTATTCTAACAGAATAACCTAAGACCAAAAGATGGTTGACCAAAGCAAGACCCAAATAAGAAACACCACTTGTAACACAAACCAAGGTTGTTCTTTTTTCTTCATCATCTTCATCTAAAGAAGAAAAATATTTGTTTTTTCTTAACCCTTCATCGTCTTTTCTCCGGTGGATACCGGCGGTAGCCACTAGATTCCGGTGAAAGGCAATAAGCTCTGATCTCTTGGTTTCACATGTGCCTACAACACCCTATTTTTTTTTATATGATATGATATGGTATTTAGTTAGGGATATGAAAAGAATGGATTGTGAAAGGTTTATATTATGGTTATGAAGGTTSAAAGGGGTTNGAAAGGAATATTTATATAATAAAAAAAGNGTGAGTGTGTTGTGTTGTATTGTGTTGTACCTAAGTTCAATNTGGGTCAAGTGAATGAACTATTCATTCATTCCCGAGTTACT
TARGET=1133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00931 Ps_contig_mira-and-tgicl-ass_franssen_8920
SEQUENCE=CASTCRSKSACAGCAAAAACCAGCCTTTTAATTTCAATTCACATAAATTAAAATAATACATTAAATTCAGTGATTCTTCTTCTTCTTCTTCTTCCTCACTCACACTATGAAATAAATAAAACATTTCAAACTCGTTTAAAATTCAGAATACAAACCCTAAATCCTAATCGTGAGCGTTGAAGCTTCGAGAATGGTTTCTCTTGAAGAATCCCGCTCGAATTCGAATCGTTTTCCTTTACCGAGAAGCTATCAGTATCATAATCATTCATCTGTTTCCTTCAAAATCGCAGCGGAATATTGGAAGATCCATGCGAACCAGAAGATCTAGTATCTTTGATGAAGATAACAGTAGTTGCACGTTTACCGAGAGATCAACGTGTGTATCGGAGAATTTAACCGATTCCGTCGTCGACCTCCGTCTCGGTGAACTCGCTTCCCGGAATATTAAATCCGGTAGGTCCTCTACGACTGAGCAGGACCTTCTCGATCTTTCACAGGCTTTCAGTGAGTATTCTGCTTGTAGTAGTGATATTTCCGGTGAGCTTCAGCGGTTGGCGACTCTGCCGTCGCCGGAATGTAATCTGAAGAGTGATGGTGGAGGTGATGGCGAGGAGGAAGTTGAGCCGGAGCCGTGCATGGGGTTTTTGCAGAGGGAGAATTTCTCGACGGAGATTATTGAGAGTATTTCGCCGGAGGATCTTCAGCCGACGGTGAAGCTTTGTATTGATGGTTTACAGTCTTCGTCGGTGGCTGTGAAACGTTCGGCGGCGGCGAAACTGAGGCTGCTTGCTAAAAGAAATAGGGCTGATAATAGAGTTCTGATTGGACG
TARGET=74,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00932 Ps_contig_mira-and-tgicl-ass_franssen_8924
SEQUENCE=TGTTACTAAATGTCCCAAATCATTTCAACACATAAACAACATCATCATCATCATTATCATTATCATTATCATTATCATCATCATGATTTCCTTAATTGATGTTTATCATGTAGTGACAGCAACTGTCCCATTATATGTAACTATGTTACTAGCTTACATTTGTAGTTAAATGGTAGGTAAACTTTCACTCCAGATCAA
TARGET=40,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00933 Ps_contig_mira-and-tgicl-ass_franssen_8941
SEQUENCE=GASGATCTAGAGAGATTGRGAATATAAATTTCCCTTCACTCAAAAAAACGAAAACCTAAAGAAAGAACATGCCAAGAAACGATGCACAGTAACAACAACAACAACATCAACAACTCAGAAGGTAGCAGAAACACCATGAGCATTCTTTCTTCCCGAACAAGCTCTCGTTCTGTAACCGAAACCGTAAACGGTTCACACAAGTTCGTAATTAAGGGATACTCTCTTGCCAAAGGGATGGGAGTTGGAAAACACATAGCCAGCGAAACTTTCACCGTCGGTGGTTACCAATGGGCTATTTACTTCTACCCCGATGGTAAAAACCCCGAGGATAATTCCGCTTATGTTTCCGTTTTCATTGCTCTTGCTTCCGAAGGAACCGATGTTCGTGCTCTTTTTGAACTCACTCTGCTTGATCAGAGTCCCAATGGAAAGCATAAGGTTCATAGTCACTTTGATCGATCTCTTGAGAGTGGTCCTTATACTCTCAAGTATAGAGGCAGCATGTGGGGATATAAGCGGTTTTTCAAACGTGCTCAACTCGAGGCATCATCCTTTCTTAAGGATGACTGCTTGAAGATAAATTGCACTGTTGGTGTTGTTGTGTCGTCCATAGATTGTTCTAAATTGACCACCATACATGTTCCTGAATCTGATATTGGATCTCATTTCGGCATGCTGTTAGAGAATGAGGAGGGATCTGATATTACTTTCTCTGTTGGCGGTGAAAGGTTTCACGCACACAAACTTGTTATGGCAGCTCGGTCAACTGCATTTGAAACTGAGTTTTTCAATAGGATGGAGGAAGATGACTGTGATGTTGTTGTTACGGACATGGAACCTAAGGTTTTCAAGGCGTTGCTTCATTTTATTTATAGAGACGCTCTTATAGAAGATGAAGAGTTCTTCATGTCAGGTTCATCGTTTCTGCCTTCAATATCTGAAACATTTGCAGCAAAGTTATTAG
TARGET=91,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00934 Ps_contig_mira-and-tgicl-ass_franssen_8989
SEQUENCE=GGTCTCACTCACACTGTAAAATTAATTTTTAACAYCTCTCTTATTTCTGTATGAAATTCCATTTCCGAAGCATTTCCGATCCAGAGAAAGAGAACCCTTTGTGACAGCTGTATCCAGAGAAAGAGATTTATAGATTAGATTTTTCGTGTGGTAATTGGTTTCTCTCTTCGCAAGTGGCGAGATCCATCTCGCTCCCACTCGCTAGGGTTTTTGATTTCGCGAGATGGCGACTTCATCTTCTTCTTCTTCAGCGTCGACGTTGTCTACGAGTTCAAGCTGGCGCGAAGGAATGTCCTACCG
TARGET=236,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00935 Ps_contig_mira-and-tgicl-ass_franssen_9006
SEQUENCE=GCGTTTACAAACACTGTAACCCCTGCGCGCCGGCGAGTCGTGTCAGGAACGGTGGAAATGGAAATTCAGAGTCGGAAACTGATTCGGAGAAGTTTCGGAGAACGAGGACATGGGGAAAGGAGATGGAAGCGAGGTTGAAAGGGATCTCGAGTGGAGAAGGAACGCCGAGTTCGTCTTCGTCGTCGTTCGGCGGTGGTAGTGGACGGAGAGTGGTGGTTTTGGAGGAGGAGGAAGGGAAGGAGTGGGTGGCTCAAGTGGAGCCTGGCGTTTTGATTACTTTTGTGTCGCTTACTCGTGGCGGGAATGATCTAAAGCGGATACGGTTCAGTCGCGATACATTTAACAAATGGCAAGCTCAAAGATGGTGGGCAGAGAACTATGACAAAGTCATGGAACTTTACAATGTTCAAAGGCTTAACCGCCAAGCTTTCCCGTTACCAACTCCAGCAAGATCTGAAGATGAGAGTTCAAAGCGTGAATCAATAGCAGACAGCCCTGTGACACCTCCACTGACCAAGGAACAACTATCGCGCAATTTATACCGACCAACAGGAATGGGAATGGGATATTCATCCTCAGATTCCTTTGATCAACACTCAATGCAGTCCAAACATTATCAGTATGATTTAAGTGGCATGAACTCAACTCCAAAGKTTCAACCATTAGTGCTGACAAAGACAGATA
TARGET=221,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00936 Ps_contig_mira-and-tgicl-ass_franssen_9013
SEQUENCE=TAARTGAAGGGAAGTTGGAGGGAGGCTCTAATAGTGCCTCCAAAAAATAGGCTTTGTAAGGAGAGGTTCAAGAATTTCAATGCATGTTTTGAAGAAATATACAGGGTTCAGACAGGTTGGAAGGTACCTGATGAGCAGCTTCGTGAAGAGATGCGAATATCTATATCTGAGAGGGTGATTCCTGCATACCGCTCATTTGTAGGGAGATTTAGCTGTCAGCTGGAAGGAAGACATTCAAGCAAATACATTAAGTATTCACCAGAGGATCTAGAGACTTTTTTATTAGATTTATTTGAAGGATCCCCTGCTGTATTGCATCATATAAAGAGAAAGAGTTCATAGTATATTAAAAGATAAGATAAGATGGTTTTGACAGGTGAACTCATTTTATCAGGATTAGTTGAAAAAGTTTTTATGTGTCATGTGTAAACTATCTACCTTCTACCTGGATAGCTGTTTATCTTACAGGTCTTAATGTTAGAGTTGGATTTTTCTCCTGCGAATACTGAAATATCTCTTGTATATATTCATAAGTCTAGTGTCTTGCTCAACTTATTTATGCTGTTATGGGTAATCACTGTCCTTGTGGAAATAATATTCATACAGTCATACAGTACTTATATTTGTTGCCTTGATTGAGTTCTGATCTCATGTAATTACATATCTTCAATTTGCATAATTTTCAGTTTTCTGTAAAT
TARGET=351,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00937 Ps_contig_mira-and-tgicl-ass_franssen_9024
SEQUENCE=CACACAAAACATTTGTTCATATATTCTCTCTCTCACTAACTTCAACAATCTCAATTTCTTCTTTCTAATCCAACCAAATTTCACACTCACACTCGTCAAATTCAAACCAAAAATGTCTGCTTTTTCCCCTCCAATACATTCACCACTTCTTCTAAACACCCAAACCAATCTTTCTTCATCTTCTTCACC
TARGET=25,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00938 Ps_contig_mira-and-tgicl-ass_franssen_9030
SEQUENCE=CGGCAGTTGTAAGACTTGTTTCGTTTGGTAGTCTGTATGAGTGTCCTGTTACTCGTGTTAAGGACTATCGATCTAGGGTTTTTGGGTTTTTGGTATTAAGTCGCTTAAGTTCTTGGATAAGATGGATGCGGAGGAGAATGAGAGGCCGGAGTCCGATGAGGAGGAGGAGGATGAAGAGGAGGAAGAAGAGGAGGAGGATCCTGGGAGTGGTGAGATTGATGGAGGCGAAGAAGATCGACAGTTTGGGATGAGCAATGGTCATAGTGAGGGTGTTGATGGAGTTGTTGATGCTGATGAGGAAGATGAGAGTGATGCGGATGAGGAGGTGACCGAGACTTCAAGAGGGAGGGTGAATGGGTTGAACCATCAGGAGAATGGGTTCCATGTTGCCCCTGCTGTGGGTGATGATGTGGAGGAGGAAGATGATGATGATGACGACGATGATGATGTTATTTTGTTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1188
PRIMER_LEFT_PENALTY=0.075753
PRIMER_RIGHT_PENALTY=0.043085
PRIMER_LEFT_SEQUENCE=ATCGATCTAGGGTTTTTGGGTT
PRIMER_RIGHT_SEQUENCE=CCTCCTCTTCTTCCTCCTCTTC
PRIMER_LEFT=66,22
PRIMER_RIGHT=193,22
PRIMER_LEFT_TM=60.076
PRIMER_RIGHT_TM=59.957
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=0.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=0.1188
PRIMER_LEFT_1_PENALTY=0.075753
PRIMER_RIGHT_1_PENALTY=0.043085
PRIMER_LEFT_1_SEQUENCE=ATCGATCTAGGGTTTTTGGGTT
PRIMER_RIGHT_1_SEQUENCE=TCCTCCTCTTCTTCCTCCTCTT
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=194,22
PRIMER_LEFT_1_TM=60.076
PRIMER_RIGHT_1_TM=59.957
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.2752
PRIMER_LEFT_2_PENALTY=0.232153
PRIMER_RIGHT_2_PENALTY=0.043085
PRIMER_LEFT_2_SEQUENCE=TGGGTTTTTGGTATTAAGTCGC
PRIMER_RIGHT_2_SEQUENCE=CCTCCTCTTCTTCCTCCTCTTC
PRIMER_LEFT_2=82,22
PRIMER_RIGHT_2=193,22
PRIMER_LEFT_2_TM=60.232
PRIMER_RIGHT_2_TM=59.957
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=0.2752
PRIMER_LEFT_3_PENALTY=0.232153
PRIMER_RIGHT_3_PENALTY=0.043085
PRIMER_LEFT_3_SEQUENCE=TGGGTTTTTGGTATTAAGTCGC
PRIMER_RIGHT_3_SEQUENCE=TCCTCCTCTTCTTCCTCCTCTT
PRIMER_LEFT_3=82,22
PRIMER_RIGHT_3=194,22
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=59.957
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=0.2830
PRIMER_LEFT_4_PENALTY=0.075753
PRIMER_RIGHT_4_PENALTY=0.207235
PRIMER_LEFT_4_SEQUENCE=ATCGATCTAGGGTTTTTGGGTT
PRIMER_RIGHT_4_SEQUENCE=GATCCTCCTCCTCTTCTTCCTC
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=60.076
PRIMER_RIGHT_4_TM=59.793
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_00939 Ps_contig_mira-and-tgicl-ass_franssen_9030
SEQUENCE=CGGCAGTTGTAAGACTTGTTTCGTTTGGTAGTCTGTATGAGTGTCCTGTTACTCGTGTTAAGGACTATCGATCTAGGGTTTTTGGGTTTTTGGTATTAAGTCGCTTAAGTTCTTGGATAAGATGGATGCGGAGGAGAATGAGAGGCCGGAGTCCGATGAGGAGGAGGAGGATGAAGAGGAGGAAGAAGAGGAGGAGGATCCTGGGAGTGGTGAGATTGATGGAGGCGAAGAAGATCGACAGTTTGGGATGAGCAATGGTCATAGTGAGGGTGTTGATGGAGTTGTTGATGCTGATGAGGAAGATGAGAGTGATGCGGATGAGGAGGTGACCGAGACTTCAAGAGGGAGGGTGAATGGGTTGAACCATCAGGAGAATGGGTTCCATGTTGCCCCTGCTGTGGGTGATGATGTGGAGGAGGAAGATGATGATGATGACGACGATGATGATGTTATTTTGTTT
TARGET=422,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00940 Ps_contig_mira-and-tgicl-ass_franssen_9045
SEQUENCE=CCAAAAAGTAAACAAACAAAAAACAAACAMTTACAACAAACAAACAACAATAGAAACTTAAAACTAAGTTTCTATATACTTCACATTTTTCCTTACTCACTCTTCTCTATTTCTGCCTCACTGTTGGTGATCACTTCCTCCTCTTTCTTTTCATCTATGGCTTCTTCTTTCTCAACGTTCTCCTCGGCTTTCTCTTTTGGTGCTTCTTCTGTGTTAACACTGTCCTTAGTTTCAGGCTCATCTATCACATTCTCATCCTTAACAACATTTTTTACTGTCTCAGATTCTGCAGTTGGTGGTTCCTGAATAACATCAACAACAGAATCAGCATTTTCTTTTGTTTCCTCAACAGGTTCCTCTTTCTCAGTTTCTTCTTCCTTCTTTGTTTCCTCTTCTGTGTGTTTCACAGTCACAGTTTCTTCTACCACCTCTTTAGTTTCAGATTCAATCACAGGTACTTTTGCTTCTGCAGTCG
TARGET=35,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00941 Ps_contig_mira-and-tgicl-ass_franssen_9046
SEQUENCE=ATTGGTGATATGGTTTGTAGCTGAATTGTTCTGTTTATTGATTGATTATAGAAATTGGAGCTACACTGTTGAATTTAGCACTGCAGGCCTATTGAGCATAACATGAGTCAAATTTGTTATGTCATAAATAAAATAAAGCTTATAGGCTCTAAATTTWAWTTTAATATACTAGGCCTTGTGTATGTATATGTAATATTTAAGGACTTGTGATATATATATATTTTAGACTAAAGT
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00942 Ps_contig_mira-and-tgicl-ass_franssen_9051
SEQUENCE=TGTTTAGTKAGAAWTTGGGAAGGGTTYTTGTGATGTGCATCCTCATCATCATCACTTTACTATCTTTTTTGAGAAAACTTTGCTAAAGCTATTTTTGGTGGCTGATTAAGACCATTTAGTGTGGACAGCAGGTGCAGGTGCAGATAATAATCTGAGTTTTTAGGTTAATGTAGAGTTAGTTAACATTTGTTTTTTTGCTAATTAGTTACTAGTTCTCAATCCACTTCACATTTTAGTTGCAAGTTAGTTA
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00943 Ps_contig_mira-and-tgicl-ass_franssen_9056
SEQUENCE=TCCGCGCGAACTGATTNAAACTTCAACACAAGTAGCTACTTCAACGCCACCCGTTTGCGAAACTGTCATGCTCGATCCAGCTCCGTAAAGTGGAATCTTGTTACCTTCCAAGTTCACTGATACAACCCGGTGACTCTTCCTCGGCTGATAGTGTTTCTTCAACTCGCCGGTTGCGATACTGATTTCTGAGTAAACAAGATTGATGGGTGAAGAGTGGACATGGATGCCAAAAAATGTAGCAGGGTTATAAATGTTCATGTGCATAGTGCTATTAAGTGTCAGAATTTTTGTTGGCACACCAGTGAAGTCTGTACCCTCCCAAACATAAAGATTATGTACTGTCAAACTCTTGACAGTAACTTCTGCTTTGTAAGGTCTGCTAGCACCCCAAATGATCAAACAAAACACAGTAAAAACAACAACAAAAGTAAAAACAGCAATCAAAGCTTGAAAGCGCCTAGCGAAAGCCTTATCCTCGAATTCGCGATAAGACCCTTCTTCAAGAATCACACCACACTCCGGCCAACCTTTATCATTCCTCTTCCTACCAACTTTCCTACTCGAAGACGACCTGAAAATCCCCGAAAACCTACTACCAGACGAGTTCCTCGAGTGGCGCCCGAACGAAGGATGCGAAGGCGACTCCATAGGACTATTTGATATTGGAGTAGCATGCATGACAGATGACTTATCTCCATCATGTGAATCTCTTGAAGGACTTTGTACATAGTATACAGGTCTCTTCGGAGATCTTGATGGAGATGAAGGAGCTAAACTAGTTATATCAGATTCTGATTTTGCAGATTGCATAGTTACTGATGTAAATTATAATGTACACACTGTGACACACTAAGAAGTTTATGCATGCATGCGTGTATATATAATATATCAGAAAAACTTAGAAATGGAAACAAATGATATCATCCCCCGTACT
TARGET=861,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00944 Ps_contig_mira-and-tgicl-ass_franssen_9075
SEQUENCE=ATAATTANAGANATAGAGGGGAAGTTTACTTTACTAATGCCAAGATTGCAGTCAATTACCATTCAATGTTTGGAAGCACTTCAATATTTACATAACCTTGGACTAATACATTGTGACTTGAAGCCGGAGAATATTTTGGTTAAAAGCTATAGCCGATGCGAGGTGAAGGTCATTGATCTTGGAAGTAGTTGTTTTGAAACAGATCACCTTTGCTCATATGTTCAGTCCAGGTCCTATCGTGCTCCCGAGGTTATTCTCGGGCTTTCATATGACAAGAAGATTGATATCTGGTCCCTTGGATGCATCCTGGCAGAACTTTGTACTGGCAATGTCTTATTCCAAAATGATTCCCCTGCAACATTACTAGCTAGGGTAATCGGTATTATCGGTCCAATTGGTCAAAGTATGCTAGCAAAAGGACGGGATACATATAAGTACTTCACAAAAAATCATATGCTTTATGAAAGGAATCAGGAAAGCAACAGGTTGGAATACCTGATTCCGAAAAAGTCGTCCTTGAGGCATAGATTACCGATGGGGGACCAAGGTTTCATTGACTTTGTTGCTCATTTGCTCGAGGTTAACTCGAAGAAGCGGCCTTCTGCATCCGAGGCTCTAAAGCACCCGTGGTTATCATACCCTTATGAACCAATATCATCTTGAAGCACACATGGTGATAATGTCAATACTACCCTCGAGAAAGGCACCACCTTTGCATCCATGCTGCTTTTCATTATCAAAATTTTAAAGATGATATGGAAAGAGTTGTGCACTTTCACATTCTTGAGAAGTTTTTTATTTTGTGGGTTATTCTTGTCATTTCCCTCCCCCCTCCAAGTTGTTAACCATGGTCAAGGATGTAATTTTAGAACAACATGTGATGTAATGTTTAGGCTATTGGTTTGTTTGTTTTGGTGTGTGATGGAAAGGGAAGTCCTTGAAATCTTTTGGTTGTACATGATGTGGGATAATTCTTTTTAGTCTGTTTAGTAGTGCTTTTGTAGGATACTATTATTGTTGGAAGAACAAGTTAATTCTAAGGTGAGGGCAATGTAAGACTCAATTAACATGAC
TARGET=901,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0769
PRIMER_LEFT_PENALTY=0.054514
PRIMER_RIGHT_PENALTY=0.022341
PRIMER_LEFT_SEQUENCE=TGGGTTATTCTTGTCATTTCCC
PRIMER_RIGHT_SEQUENCE=ATCCCACATCATGTACAACCAA
PRIMER_LEFT=803,22
PRIMER_RIGHT=968,22
PRIMER_LEFT_TM=60.055
PRIMER_RIGHT_TM=59.978
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.3442
PRIMER_LEFT_1_PENALTY=0.054514
PRIMER_RIGHT_1_PENALTY=0.289736
PRIMER_LEFT_1_SEQUENCE=TGGGTTATTCTTGTCATTTCCC
PRIMER_RIGHT_1_SEQUENCE=GATTTCAAGGACTTCCCTTTCC
PRIMER_LEFT_1=803,22
PRIMER_RIGHT_1=944,22
PRIMER_LEFT_1_TM=60.055
PRIMER_RIGHT_1_TM=60.290
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=142
PRIMER_PAIR_PENALTY_2=0.4111
PRIMER_LEFT_2_PENALTY=0.054514
PRIMER_RIGHT_2_PENALTY=0.356592
PRIMER_LEFT_2_SEQUENCE=TGGGTTATTCTTGTCATTTCCC
PRIMER_RIGHT_2_SEQUENCE=GAGTCTTACATTGCCCTCACCT
PRIMER_LEFT_2=803,22
PRIMER_RIGHT_2=1060,22
PRIMER_LEFT_2_TM=60.055
PRIMER_RIGHT_2_TM=59.643
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.5682
PRIMER_LEFT_3_PENALTY=0.054514
PRIMER_RIGHT_3_PENALTY=0.513718
PRIMER_LEFT_3_SEQUENCE=TGGGTTATTCTTGTCATTTCCC
PRIMER_RIGHT_3_SEQUENCE=CCCACATCATGTACAACCAAAA
PRIMER_LEFT_3=803,22
PRIMER_RIGHT_3=966,22
PRIMER_LEFT_3_TM=60.055
PRIMER_RIGHT_3_TM=60.514
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.7118
PRIMER_LEFT_4_PENALTY=0.689440
PRIMER_RIGHT_4_PENALTY=0.022341
PRIMER_LEFT_4_SEQUENCE=TTATTCTTGTCATTTCCCTCCC
PRIMER_RIGHT_4_SEQUENCE=ATCCCACATCATGTACAACCAA
PRIMER_LEFT_4=807,22
PRIMER_RIGHT_4=968,22
PRIMER_LEFT_4_TM=59.311
PRIMER_RIGHT_4_TM=59.978
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_00945 Ps_contig_mira-and-tgicl-ass_franssen_9102
SEQUENCE=TACKCGGRKGATTGAAATTTAGTTTGGTAGGATGAGGAAGAGACGCATTCATCCATTCGAGATTGAAGGAAGTAGCTCTTTAACAAATCCATGAGAAGAAAAGGAGAAAAGTTTATTATCAATGAAAAAAATATTAACAAGTAACAACAATAATATTAAATATTAAGCTATGAAATTGAAATTACTTCCATCAACAATTTCATCCCATTCCTTTGCACTCCGTCGTGATTCCAATTACAATGCTTCTTCTTCTTCCTCCTCCGTTCACAGACACAAGCACAGTTTCATAGTAGCCTCCAAAAGCAAAAAGCACCGTCACAATGACGCTAAGGGTTCCAATTCTCAACCAAGTCCTCCCAGAATCACTTCAAACGCCAAGCAAAACCTTCAAATCCTCAAGACATGGAAAGGAAAGTAACGAAAAAACGAAAATCTACCGCTCCAAGACCTTCCACCAGTTACCGCAA
TARGET=243,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00946 Ps_contig_mira-and-tgicl-ass_franssen_9104
SEQUENCE=CCGGTTTCCAACAAACCAAGACCTTGGCATGAAAAGGGACGAAAGAACCAGAGGGTAAAAATTACCCGGTAAAGGGTGGAGTGAGAGGATTTTGGTAGCTTGTGTTAATTTTCTACAACAGCATTAACCATTGTGAAAGGTGATGCCGTTATCAAATGCCCTAAAACTGCGTTCGGGGCACTGGCACCATAGGGTCAGTTTTATGTGCAGTGTGTTTACATTCAGGTTTAAAAATGGTAGCACATAGTACAGTATAATGGTCTGCTAGAGTTTWGGTGAATTTGCTTTCTTTTTTTAGGCTTGKTTTKGTTTCATTATTTATTAATTATTGTTTATATTATTATCTATTTATTACTGTTATATATATATTCACAGTCTTGTCGTTGACTTAGTTTTAG
TARGET=359,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00947 Ps_contig_mira-and-tgicl-ass_franssen_9105
SEQUENCE=GTCGGGGTATTCSATTTTAAAGACTATCAAAAACCTCACTCACTSACACAGAATTCTCTCCCCCTCTCTCTCTCAACATACCAACCACATACTCTCTCTTTCTCACTAGAACCATCTCAGGGAGAGAGTGACTGATCGATGATGTCTTCCTCCGGCACCGGCAGGTACATGGCTTTCCCGCCGTCGCCATCACACTCACTCCCTCATCTATCCGCTCTTCGTTCTCCTGCTTCAACTGCCATCGCTGAACATGACAAATATCTAAGTGAGTTATTGGGAGAGCGTCAGAAGATTAGTCCGTTCATGGCTGTGCTTCCTCACTGCTATCGATTGTTAAACCAAGAAATATTGCGTGTAACAACAATCTTGGGGAATGCGTCATCAGTATTAGGTCAAAGTGGGCTTGAGCACGGTAGCCCCCTGGCTACTGGCGGGATGTTCTCCAAAGGAAGTGCTGATCCGAACGGATGGGTATCACGATTTCAATCAGAAATGCCGAGTCTGATACAGTCGTCACCTACACCAAACTGGCTGAGTCCACAAAGTAGCTCGTCCGGTCTTCTTGTTAAAAAGACAATCAGAGTGGATATACCCGTGGAGACATTTCCTAACTTTAATTTTGTTGGTCGCCTCCTAGGCCCGAGAGGAAACTCTCTGAAGCGCGTGGAAGCGAACACTGAGTGCCGTGTCTTGATCAGGGGCCGAGGTAGCATCAAAGATACAGCTAGGGAGGAGATGATGAGAGGTAAACCTGGGTATGAGCATCTGAATGAACCTCTCCATATTCTAGTCGAGGCAGAATTACCGGCTGAAATAATAGATGCTCGCCTGATGCAGGCACGTGAAATACTCGAAGATTTGCTTAGGCCTGTGGAAGAATCCCATGATTTCTACAAGAAGCAGCAGCTTCGCGAACTTGCGATGATCAATGGCACTCTCCGCGAGGAGGATTCTCCCATGTCTGGTTCTGTTTCGCCGTTCAACAACAGCCTTGGCATGAAAAGGGCAAAGACCAGAGGGTAAAAAATTCCCGGTAAAGGGTGAGTGAGAGGATTTTGGAGCTTGTGTTAATTTTCTACAACAGCATTAACCATTGTGAAAGGTGATGCCGTTATCAAATGCCCTAAAACTGCGTTCGGGGCACTGGCACCATAGGGTCAGTTTTATGTGCAGTGTGTTTACATTCAGGTTTAAAAATGGTAGCACATAGTACAGTATAATGGTCTGCTAGAGTTTGGTGAATTTGCTTCTTTTTTTAGGCTGTTTGGTTTCATATTTATTAATTATGTTTATATTATTATCATTATTACTGTTATATATATATTCACAGCTTGCGTTGACAGTTTTGATATGGTTTTGCAATATAATGCATGGTTTGTACCCTTTTGTAMG
TARGET=64,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00948 Ps_contig_mira-and-tgicl-ass_franssen_9105
SEQUENCE=GTCGGGGTATTCSATTTTAAAGACTATCAAAAACCTCACTCACTSACACAGAATTCTCTCCCCCTCTCTCTCTCAACATACCAACCACATACTCTCTCTTTCTCACTAGAACCATCTCAGGGAGAGAGTGACTGATCGATGATGTCTTCCTCCGGCACCGGCAGGTACATGGCTTTCCCGCCGTCGCCATCACACTCACTCCCTCATCTATCCGCTCTTCGTTCTCCTGCTTCAACTGCCATCGCTGAACATGACAAATATCTAAGTGAGTTATTGGGAGAGCGTCAGAAGATTAGTCCGTTCATGGCTGTGCTTCCTCACTGCTATCGATTGTTAAACCAAGAAATATTGCGTGTAACAACAATCTTGGGGAATGCGTCATCAGTATTAGGTCAAAGTGGGCTTGAGCACGGTAGCCCCCTGGCTACTGGCGGGATGTTCTCCAAAGGAAGTGCTGATCCGAACGGATGGGTATCACGATTTCAATCAGAAATGCCGAGTCTGATACAGTCGTCACCTACACCAAACTGGCTGAGTCCACAAAGTAGCTCGTCCGGTCTTCTTGTTAAAAAGACAATCAGAGTGGATATACCCGTGGAGACATTTCCTAACTTTAATTTTGTTGGTCGCCTCCTAGGCCCGAGAGGAAACTCTCTGAAGCGCGTGGAAGCGAACACTGAGTGCCGTGTCTTGATCAGGGGCCGAGGTAGCATCAAAGATACAGCTAGGGAGGAGATGATGAGAGGTAAACCTGGGTATGAGCATCTGAATGAACCTCTCCATATTCTAGTCGAGGCAGAATTACCGGCTGAAATAATAGATGCTCGCCTGATGCAGGCACGTGAAATACTCGAAGATTTGCTTAGGCCTGTGGAAGAATCCCATGATTTCTACAAGAAGCAGCAGCTTCGCGAACTTGCGATGATCAATGGCACTCTCCGCGAGGAGGATTCTCCCATGTCTGGTTCTGTTTCGCCGTTCAACAACAGCCTTGGCATGAAAAGGGCAAAGACCAGAGGGTAAAAAATTCCCGGTAAAGGGTGAGTGAGAGGATTTTGGAGCTTGTGTTAATTTTCTACAACAGCATTAACCATTGTGAAAGGTGATGCCGTTATCAAATGCCCTAAAACTGCGTTCGGGGCACTGGCACCATAGGGTCAGTTTTATGTGCAGTGTGTTTACATTCAGGTTTAAAAATGGTAGCACATAGTACAGTATAATGGTCTGCTAGAGTTTGGTGAATTTGCTTCTTTTTTTAGGCTGTTTGGTTTCATATTTATTAATTATGTTTATATTATTATCATTATTACTGTTATATATATATTCACAGCTTGCGTTGACAGTTTTGATATGGTTTTGCAATATAATGCATGGTTTGTACCCTTTTGTAMG
TARGET=1314,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00949 Ps_contig_mira-and-tgicl-ass_franssen_9107
SEQUENCE=AGAGAATTTTGGGTTAAGGTTGCTGAGGTGTAAATAGTTCTGTAAAATAAGGTTCATCCAAAAGCCTTCATCTCTTGTTTATATATATATGTGTGCCATGGATAATAAAAAGGGGAAATGTTTGTGCTGGCAAC
TARGET=80,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5338
PRIMER_LEFT_PENALTY=0.121635
PRIMER_RIGHT_PENALTY=0.412146
PRIMER_LEFT_SEQUENCE=GAATTTTGGGTTAAGGTTGCTG
PRIMER_RIGHT_SEQUENCE=CCCTTTTTATTATCCATGGCAC
PRIMER_LEFT=3,22
PRIMER_RIGHT=113,22
PRIMER_LEFT_TM=59.878
PRIMER_RIGHT_TM=59.588
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=111
PRIMER_PAIR_PENALTY_1=0.8167
PRIMER_LEFT_1_PENALTY=0.404566
PRIMER_RIGHT_1_PENALTY=0.412146
PRIMER_LEFT_1_SEQUENCE=GGGTTAAGGTTGCTGAGGTGTA
PRIMER_RIGHT_1_SEQUENCE=CCCTTTTTATTATCCATGGCAC
PRIMER_LEFT_1=10,22
PRIMER_RIGHT_1=113,22
PRIMER_LEFT_1_TM=60.405
PRIMER_RIGHT_1_TM=59.588
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=104
PRIMER_PAIR_PENALTY_2=0.8727
PRIMER_LEFT_2_PENALTY=0.460533
PRIMER_RIGHT_2_PENALTY=0.412146
PRIMER_LEFT_2_SEQUENCE=ATTTTGGGTTAAGGTTGCTGAG
PRIMER_RIGHT_2_SEQUENCE=CCCTTTTTATTATCCATGGCAC
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=113,22
PRIMER_LEFT_2_TM=59.539
PRIMER_RIGHT_2_TM=59.588
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=1.3740
PRIMER_LEFT_3_PENALTY=0.961875
PRIMER_RIGHT_3_PENALTY=0.412146
PRIMER_LEFT_3_SEQUENCE=GAGAATTTTGGGTTAAGGTTGC
PRIMER_RIGHT_3_SEQUENCE=CCCTTTTTATTATCCATGGCAC
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=113,22
PRIMER_LEFT_3_TM=59.038
PRIMER_RIGHT_3_TM=59.588
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=1.8213
PRIMER_LEFT_4_PENALTY=1.409123
PRIMER_RIGHT_4_PENALTY=0.412146
PRIMER_LEFT_4_SEQUENCE=GGGTTAAGGTTGCTGAGGTGT
PRIMER_RIGHT_4_SEQUENCE=CCCTTTTTATTATCCATGGCAC
PRIMER_LEFT_4=10,21
PRIMER_RIGHT_4=113,22
PRIMER_LEFT_4_TM=60.409
PRIMER_RIGHT_4_TM=59.588
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=104
=
PRIMER_SEQUENCE_ID=SSR_00950 Ps_contig_mira-and-tgicl-ass_franssen_9112
SEQUENCE=CATGCCAAAGTTCTGATAAGGTTGTGTGTGTGGAACAAAGAACTCAACTTCACTATAATTAAGTTCTATTGGGTTTTTTTCTTAGAACTCAACAGTGAATTTTATATATGCTAATTTCCTATTGAAATGGCTCTTCCTCATCTACTTCTTCTGCTTATTCTTCATTGCTCATTGTTTGTAGCATTGGTATCTGGCTGGAAGACATTGCAAGGATCACCACCGGTGGTAATAGCACGCGGCGGATTTTCGGGGTTATATCCAGATTCAAGTTTACTTTCTTATAACTTGG
TARGET=23,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8881
PRIMER_LEFT_PENALTY=1.736200
PRIMER_RIGHT_PENALTY=0.151911
PRIMER_LEFT_SEQUENCE=CATGCCAAAGTTCTGATAAGGT
PRIMER_RIGHT_SEQUENCE=CAGCCAGATACCAATGCTACAA
PRIMER_LEFT=0,22
PRIMER_RIGHT=196,22
PRIMER_LEFT_TM=58.264
PRIMER_RIGHT_TM=60.152
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=1.9440
PRIMER_LEFT_1_PENALTY=1.736200
PRIMER_RIGHT_1_PENALTY=0.207756
PRIMER_LEFT_1_SEQUENCE=CATGCCAAAGTTCTGATAAGGT
PRIMER_RIGHT_1_SEQUENCE=AGATGAGGAAGAGCCATTTCAA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=142,22
PRIMER_LEFT_1_TM=58.264
PRIMER_RIGHT_1_TM=60.208
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=2.0408
PRIMER_LEFT_2_PENALTY=1.736200
PRIMER_RIGHT_2_PENALTY=0.304636
PRIMER_LEFT_2_SEQUENCE=CATGCCAAAGTTCTGATAAGGT
PRIMER_RIGHT_2_SEQUENCE=CTTGAATCTGGATATAACCCCG
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=268,22
PRIMER_LEFT_2_TM=58.264
PRIMER_RIGHT_2_TM=59.695
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=2.2445
PRIMER_LEFT_3_PENALTY=1.736200
PRIMER_RIGHT_3_PENALTY=0.508338
PRIMER_LEFT_3_SEQUENCE=CATGCCAAAGTTCTGATAAGGT
PRIMER_RIGHT_3_SEQUENCE=TGAATCTGGATATAACCCCGAA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=58.264
PRIMER_RIGHT_3_TM=60.508
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=2.4499
PRIMER_LEFT_4_PENALTY=1.736200
PRIMER_RIGHT_4_PENALTY=0.713664
PRIMER_LEFT_4_SEQUENCE=CATGCCAAAGTTCTGATAAGGT
PRIMER_RIGHT_4_SEQUENCE=CTGGATATAACCCCGAAAATCC
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=261,22
PRIMER_LEFT_4_TM=58.264
PRIMER_RIGHT_4_TM=60.714
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_00951 Ps_contig_mira-and-tgicl-ass_franssen_9117
SEQUENCE=AATGCTGGAGAATGATTTGGACTATATCCTTCAGAATGATTTGGACTATATCCTGAAGAAGGATTTGGATTATGGTTGGGCCATTGATTCATGCCTGGTGCATTGTTGTATGATGGACCGCCCGGTGGTTCAAACCTAGGACTTGGATAAGTAGCACCACCTGATGGACGGAATGGAAAGTTATACTGAGGCGGGCCTCTGGGTCCATTCCAAACTGGGTTTCTATAGGGTGCTGGCAATGCTTGAGTGGGAGAAGGAGACATCTGTGGATTTGCTGATTCTGTATGTGGCAACGAAAACCGCACCATAGGAGATCCACCAAAATTTAGAGGAAGATGAGGAGGAGGAGGAGGC
TARGET=338,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00952 Ps_contig_mira-and-tgicl-ass_franssen_9119
SEQUENCE=AGACACCAGCATGTCTTTAATTATGGATGGAAGTGCGGCTTCATTTTCAACTCAAGAGTCTGATTTTTCAACTGGTTATTTAGAAGATGCTTTGGTTGAGTTTGGTGAAAGTTCTAAACGTAGACGTTTACTGCCATATACTGATACTGATGATGAACAAAGCAAGAGTAGTATTACTACTACTACTACTACTTCCATGGATGATTTTGATAAGAATTTCTGGAATTTCAACCCTATATGGAACCAACCAGTTGAGAATTYCTACTGCATGGATCAAATTGAAAGGATTTGTGGATTTTCAGATGAGCTTATAAGTCCATTAGAGGAGTAGAATGAATGAGCAACA
TARGET=175,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00953 Ps_contig_mira-and-tgicl-ass_franssen_9140
SEQUENCE=GATTAAAAATATTAATAATTACTCCCTTTTCCCTTCTTTCTTTATTCTTTTCTTTTCTTTTCTTTCTTCCCTCTTTCCTTCTTCCTACTTTTTAATGTTCTTTTAGGGAAGGAAAAAAAAAAA
TARGET=45,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00954 Ps_contig_mira-and-tgicl-ass_franssen_9147
SEQUENCE=ACAATTCAATTTTTCCACACAAATATGGACTCATTATTATTAGTTACTATTATTCCATATTAATATTAATAAACACGTAATATATATTTTCAGGCTTGATTCCATAGATAAATAAATAAATATATCTTCGATAAMRTCKKGGTATTCCTGAAGTGGCAAAACAAAATTCATCTTTTAGTTTTATTTCATTACAAGATAGACTCTAAGAAGAGTGAGAACATAAAATCATCATAACGAAACCCTAATTCCCAAATCCTAGACTCAATCGATTTCAATGGAGGGAATGGTATCTGCCATTGATCAACAAGCATTGGTCTCTTCTTTCCTGGAAGTCGCTCAGGGCCAAACCGCCGCCACCGCTAGACAGTTTCTTCAGGCTACGAGTTGGAAACTTGAGGAAGCTCTTCAGTTTGTTCTTGATTGGGAGTGAATCTGGGGTGGTGCTACCGCCGGCACCATCGTTCTTTACTCCACCTTTAAGAAAA
TARGET=108,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00955 Ps_contig_mira-and-tgicl-ass_franssen_9150
SEQUENCE=AAATAATCTCTTCTTATTTGTTGGTAAGGAATTACTTGTAAGAAACAAGAAGAATGAGCTGACTGAATTGATAAAGAAAAGGAAGTCTGTTAAAGTGGGCGGGTGTGAGCAAAGTTCTCTGTCAAGATTTGAGCAAACATTGCAGTCAGACGAGTGTATCGCGCGAGGCGGAGGTTGATGACGATGATGATGATGATGACGACGAAGATGACGGGGAGCTCTAAAACCCTGGATAATATGGTGGCTGAAAATATCTGCTTTGGGCAATTAAAAGTTAACAGACTGCATCACAAAATTGATGGTGCTGTCCTTGTTGTAGATGGAGTTAGAATGACTTCCATTTTTCTTAATTAATTAAAAGACTATTTTAATGATTATAGGTTAATCAGACTCCTAAWCAAATTATTAATTTAAAGAAAAACT
TARGET=183,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00956 Ps_contig_mira-and-tgicl-ass_franssen_9150
SEQUENCE=AAATAATCTCTTCTTATTTGTTGGTAAGGAATTACTTGTAAGAAACAAGAAGAATGAGCTGACTGAATTGATAAAGAAAAGGAAGTCTGTTAAAGTGGGCGGGTGTGAGCAAAGTTCTCTGTCAAGATTTGAGCAAACATTGCAGTCAGACGAGTGTATCGCGCGAGGCGGAGGTTGATGACGATGATGATGATGATGACGACGAAGATGACGGGGAGCTCTAAAACCCTGGATAATATGGTGGCTGAAAATATCTGCTTTGGGCAATTAAAAGTTAACAGACTGCATCACAAAATTGATGGTGCTGTCCTTGTTGTAGATGGAGTTAGAATGACTTCCATTTTTCTTAATTAATTAAAAGACTATTTTAATGATTATAGGTTAATCAGACTCCTAAWCAAATTATTAATTTAAAGAAAAACT
TARGET=347,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00957 Ps_contig_mira-and-tgicl-ass_franssen_9151
SEQUENCE=TGACGCAGTCTGTTAACTTTTAATTGCCCAAAGCAGATATTTTTCTAGCCACCATATTATCCAGGGTTTTAGAGCTCCCCGTCATCTTCGTCGTCATCATCATCATCGTCATCAACCTCCGCCTCGCGCGATACACTCGTCTGACTGCAATGTTTGCTCAAATCTTGACAGAGAACTTTGCTCACACCGCCCACTTYAACAGATTCCTTTTCTTTATCAATTCAGTCAGCTCATCTTCTGTTTCTCAAAGTAATCTCCACAATAAG
TARGET=94,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00958 Ps_contig_mira-and-tgicl-ass_franssen_9166
SEQUENCE=GKCGGGGATAACATATATGAGAGATTTTCTTCTTGACAAATTACAAGCAATCATGGATGGAAAAAGTAAAACCTATTGGGTTGATAAGGATGATGATGATGATGTAACTGATTCAATTTCTAATGGATATATGACAGAAGATTCAATGCGTTCATTTTTTCTTCATCAGAGATGGATGATGATGATCAAGAAGTTTCATCATCTTA
TARGET=89,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00959 Ps_contig_mira-and-tgicl-ass_franssen_9166
SEQUENCE=GKCGGGGATAACATATATGAGAGATTTTCTTCTTGACAAATTACAAGCAATCATGGATGGAAAAAGTAAAACCTATTGGGTTGATAAGGATGATGATGATGATGTAACTGATTCAATTTCTAATGGATATATGACAGAAGATTCAATGCGTTCATTTTTTCTTCATCAGAGATGGATGATGATGATCAAGAAGTTTCATCATCTTA
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00960 Ps_contig_mira-and-tgicl-ass_franssen_9167
SEQUENCE=TGGTTTTTCTTTCTTAGGAAGATCTTCTATGTTTTCCTACCCTTGCTTTAAAGTACCCAAAAGATTGTGCCTTCCCTTGGTAAAACATAGACAATCCGCTCTTCATAGGAAGATGGTTCATGAGCTCTGATAATTCATATAAAGGACCATTTAAATTTGATGATGATGAAGATGATAAACAGAAGTTGAAGNTGATGAAACTTCTTGATCATCATACATCCATCTCGGATGAAANAAAATNTGAACACTATTGATTCTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3381
PRIMER_LEFT_PENALTY=0.122569
PRIMER_RIGHT_PENALTY=0.215550
PRIMER_LEFT_SEQUENCE=GTACCCAAAAGATTGTGCCTTC
PRIMER_RIGHT_SEQUENCE=TTTCATCCGAGATGGATGTATG
PRIMER_LEFT=52,22
PRIMER_RIGHT=233,22
PRIMER_LEFT_TM=59.877
PRIMER_RIGHT_TM=59.784
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=0.4185
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.295944
PRIMER_LEFT_1_SEQUENCE=GTACCCAAAAGATTGTGCCTTC
PRIMER_RIGHT_1_SEQUENCE=TTCATCCGAGATGGATGTATGA
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=60.296
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=0.6637
PRIMER_LEFT_2_PENALTY=0.448106
PRIMER_RIGHT_2_PENALTY=0.215550
PRIMER_LEFT_2_SEQUENCE=CCCTTGCTTTAAAGTACCCAAA
PRIMER_RIGHT_2_SEQUENCE=TTTCATCCGAGATGGATGTATG
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=233,22
PRIMER_LEFT_2_TM=59.552
PRIMER_RIGHT_2_TM=59.784
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.7441
PRIMER_LEFT_3_PENALTY=0.448106
PRIMER_RIGHT_3_PENALTY=0.295944
PRIMER_LEFT_3_SEQUENCE=CCCTTGCTTTAAAGTACCCAAA
PRIMER_RIGHT_3_SEQUENCE=TTCATCCGAGATGGATGTATGA
PRIMER_LEFT_3=39,22
PRIMER_RIGHT_3=232,22
PRIMER_LEFT_3_TM=59.552
PRIMER_RIGHT_3_TM=60.296
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=0.8001
PRIMER_LEFT_4_PENALTY=0.584504
PRIMER_RIGHT_4_PENALTY=0.215550
PRIMER_LEFT_4_SEQUENCE=AAAGTACCCAAAAGATTGTGCC
PRIMER_RIGHT_4_SEQUENCE=TTTCATCCGAGATGGATGTATG
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=59.415
PRIMER_RIGHT_4_TM=59.784
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_00961 Ps_contig_mira-and-tgicl-ass_franssen_9174
SEQUENCE=CTTACTCATTCTTCATCTCAAACCCAACATGCACGCAAAACCAACTCCAGATCCAACAACAAACACTWCCTTCTTCCTTCARAACCCTCCATCACTCTCTTCTCAATCTCAACCACTTTCCACTTCATTCCCTCACCGTCCTAACCGTTCCAAGAAACCCACACCTGTCGCCGCCGCTGCCGCCGCCGCCGCAGAATCCTACCAAAAAGTTCCAGAGAGTCTTCTTCACTTCCGACGACGTCGTACTCATTCTTAAATCCCTTTCAGTATTTCAAAATCC
TARGET=179,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00962 Ps_contig_mira-and-tgicl-ass_franssen_9193
SEQUENCE=GTCGGGGATACTACCTCACCCAACTTCATACATTGTTCATTCAAACTTCCCTATTCTTCCTTTCTTCAAATAAGCACTTAATATCAGAAGCGCGTTTATAACTTAACTTATAACAACACAAAAGAACTTGTTTTGTCATTAGTGTGAGTAGCATGGGTATTGAAAGAGCAGGAGGAGGAGGGCTTAAGAGTTTTCGAAGCGGATGGAGTGTGCCACCTAAGCTATGTGATTCTTGCAAGCTTTCTTCTGCCGCGCTTTTTTGCCATTCTGATTCCGCGTTTCTATGTATCAACTGTGATTCCAGGATTCATTCTGGGAACAAGCTTTCCACGCGCCATGAGCGCGTGTGGATGTGCGAAGTTTGCGAACAAGCACCAGCCTCCGTCACGTGTAAAGCGGACGCCGCTGCTCTCTGCGTCACGTGCGATTCTGACATCCACTCAGCGAATCCACTCGCCCGCCGCCACGAGCGTGTCCCCGTCGAGCCTTTCTTCGACTCTGCCGAATCCGTTGTGAAATCCTCCGCCGCTGCTGCCGCTTCCTTCAATTTCGTCGTACCTACCGACGACGGTTTCAGCCAAGACGACGCCGAAGCATCTACGTGGTTGATTCCGAATCTGAATCCGAATTTCGGATCTAAACTAACCGAAACTCCAGATATCAAAACCAGAGATATGTTCTTCTCCGATATGGATCCGTTTCTCGATTTCGATTACTCTAACAACTTCCAGAACAATAATTGCAACGCGATAAACGATAGCGTAGTTCCGGTTCAAACCAAACCTACTCCGGCACCGATGATGAATCATCATAACTCAGAAGCTTGCTTCGACATCGATTTTTGCAGATCGAAGCTATCCTCGTTTAACTATCCATCACATTCTATCAGCCAAAGCGTTTCTTCTTCTTCGCTTGACGTCGGAGTGGTGCCGGACGGGAACGTGGTGTCGGAGTTTTCATATACTTTCGGTTCGGAATCAATGGTGTCAGGTGGTGTTAGTAGCAACAACCAAGGGGTGCAAGGAGCAACTCAACTATGTGGAATAGATCGTGAAGCGAGGGTTCTAAGATACAGAGAGAAGAGGAAGAACCGTAAATTCGAAAAAACAATTCGTTATGCTTCGCGAAAAGCTTATGCAGAAACCCGGCCTAGAATCAAAGGCCGGTTCGCGAAACGAACCGAAATTGACTCGGATATAGACGGTCTCTATAATAACCCTGCAGATCCTCTCACCATCCCTGCATCACTTTTAATCGATTCCCCCTACGGCGTTGTCCCGACATTTTAGTAAACAATATCTGATGTTATTGCATAACTATCGATTAAGCCCGCAAAGAAATTGAAATGGATGAATATGAATCTCCTATAATGGTGTTTATGGGAACAGTTGATTATTATTATGATGCATGATGTATGAATTTGTCTAGAGTAAAAAAAAGGAGTATTATTACTTTTTTAGTTGAATTGAAAAGAAGGAAAGAGTTTAGGGGAGTTTTTGGTGATAGTATTAATCTGGAGTAGAAGTAGAATGTGTACTGTAAACTCAATTTCACTGTTTAATGTCCTATTTTGAGTAAAAAAAGAAGTGTCTTTTTCTAAAGGGATCACGGATCTCTTTCCTCGTTTTTATTGGATGGGGTGGCACTCCCCAACTGTGTATGTGTATCCGTGTTTTCTTTTTCTGAATGATTAATATATTAACTTCCAGTAAAMC
TARGET=170,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00963 Ps_contig_mira-and-tgicl-ass_franssen_9193
SEQUENCE=GTCGGGGATACTACCTCACCCAACTTCATACATTGTTCATTCAAACTTCCCTATTCTTCCTTTCTTCAAATAAGCACTTAATATCAGAAGCGCGTTTATAACTTAACTTATAACAACACAAAAGAACTTGTTTTGTCATTAGTGTGAGTAGCATGGGTATTGAAAGAGCAGGAGGAGGAGGGCTTAAGAGTTTTCGAAGCGGATGGAGTGTGCCACCTAAGCTATGTGATTCTTGCAAGCTTTCTTCTGCCGCGCTTTTTTGCCATTCTGATTCCGCGTTTCTATGTATCAACTGTGATTCCAGGATTCATTCTGGGAACAAGCTTTCCACGCGCCATGAGCGCGTGTGGATGTGCGAAGTTTGCGAACAAGCACCAGCCTCCGTCACGTGTAAAGCGGACGCCGCTGCTCTCTGCGTCACGTGCGATTCTGACATCCACTCAGCGAATCCACTCGCCCGCCGCCACGAGCGTGTCCCCGTCGAGCCTTTCTTCGACTCTGCCGAATCCGTTGTGAAATCCTCCGCCGCTGCTGCCGCTTCCTTCAATTTCGTCGTACCTACCGACGACGGTTTCAGCCAAGACGACGCCGAAGCATCTACGTGGTTGATTCCGAATCTGAATCCGAATTTCGGATCTAAACTAACCGAAACTCCAGATATCAAAACCAGAGATATGTTCTTCTCCGATATGGATCCGTTTCTCGATTTCGATTACTCTAACAACTTCCAGAACAATAATTGCAACGCGATAAACGATAGCGTAGTTCCGGTTCAAACCAAACCTACTCCGGCACCGATGATGAATCATCATAACTCAGAAGCTTGCTTCGACATCGATTTTTGCAGATCGAAGCTATCCTCGTTTAACTATCCATCACATTCTATCAGCCAAAGCGTTTCTTCTTCTTCGCTTGACGTCGGAGTGGTGCCGGACGGGAACGTGGTGTCGGAGTTTTCATATACTTTCGGTTCGGAATCAATGGTGTCAGGTGGTGTTAGTAGCAACAACCAAGGGGTGCAAGGAGCAACTCAACTATGTGGAATAGATCGTGAAGCGAGGGTTCTAAGATACAGAGAGAAGAGGAAGAACCGTAAATTCGAAAAAACAATTCGTTATGCTTCGCGAAAAGCTTATGCAGAAACCCGGCCTAGAATCAAAGGCCGGTTCGCGAAACGAACCGAAATTGACTCGGATATAGACGGTCTCTATAATAACCCTGCAGATCCTCTCACCATCCCTGCATCACTTTTAATCGATTCCCCCTACGGCGTTGTCCCGACATTTTAGTAAACAATATCTGATGTTATTGCATAACTATCGATTAAGCCCGCAAAGAAATTGAAATGGATGAATATGAATCTCCTATAATGGTGTTTATGGGAACAGTTGATTATTATTATGATGCATGATGTATGAATTTGTCTAGAGTAAAAAAAAGGAGTATTATTACTTTTTTAGTTGAATTGAAAAGAAGGAAAGAGTTTAGGGGAGTTTTTGGTGATAGTATTAATCTGGAGTAGAAGTAGAATGTGTACTGTAAACTCAATTTCACTGTTTAATGTCCTATTTTGAGTAAAAAAAGAAGTGTCTTTTTCTAAAGGGATCACGGATCTCTTTCCTCGTTTTTATTGGATGGGGTGGCACTCCCCAACTGTGTATGTGTATCCGTGTTTTCTTTTTCTGAATGATTAATATATTAACTTCCAGTAAAMC
TARGET=899,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00964 Ps_contig_mira-and-tgicl-ass_franssen_9194
SEQUENCE=GATTAAGAAAAACAGCACCCAAAAGAAAKGATACTACCTCACCCAACTTCATACATTGTTCATTCAAACTTCCCTATTCTTCCTTTCTTCAAATAAGCACTTAATATCAGAAGCGCGTTTATAACTTAACTTATAACAACACAAAAGAACTTGTCTTGTCATTAGTGTGAGTAGCATGGGTATTGAAAGAGCAGGAGGAGGAGGGCTTAAGAGTTTTCGAAGCGGATGGAGTGTGCCACCTAAGCTATGTGATTCTTGCAAGCTTTCTTCTGCCGCGCTTTTTTGTCCATTCTGATTCCGCGTTTCTATGTATCAACTGTGATTCCAGGATTCATTCTGGGAACAAGCTTTCCACGCGCCATGAGCGCGTGTGGATGTGCGAAGTTTGCGAACAAGCACCAGCCTCGGTCACGTGTAAAGCTGACGCCGCTGCTCTCTGCGTCACGTGCGATTCTGACATCCACTCAGCGAATCCACTCGCCCGCCGCCACGAGCGTGTCCCCGTCGAGCCTTTCTTCGACTCTGCCGAATCCGTCGTGAAATCCTCCGCTGCTGCTGCTGCTTCCTTCAATTTCGTCGTACCTACCGACGACGGTTTCAGCCAAGACGACGCCGAAGCATCTACGTGGTTGATTCCGAATCTGAATCCGAATTTCGGATCTAAACTAACCGAAACTCCAGATATCAAAAACCAGAGATATGTTCTTCTCCGATATGGACTTCCGTTTCTCGTATTTCGAATTACTCTAACAACTTCCAAGAACAATATTGCAACGCAATAAACGATAGCGTTACGG
TARGET=193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00965 Ps_contig_mira-and-tgicl-ass_franssen_9194
SEQUENCE=GATTAAGAAAAACAGCACCCAAAAGAAAKGATACTACCTCACCCAACTTCATACATTGTTCATTCAAACTTCCCTATTCTTCCTTTCTTCAAATAAGCACTTAATATCAGAAGCGCGTTTATAACTTAACTTATAACAACACAAAAGAACTTGTCTTGTCATTAGTGTGAGTAGCATGGGTATTGAAAGAGCAGGAGGAGGAGGGCTTAAGAGTTTTCGAAGCGGATGGAGTGTGCCACCTAAGCTATGTGATTCTTGCAAGCTTTCTTCTGCCGCGCTTTTTTGTCCATTCTGATTCCGCGTTTCTATGTATCAACTGTGATTCCAGGATTCATTCTGGGAACAAGCTTTCCACGCGCCATGAGCGCGTGTGGATGTGCGAAGTTTGCGAACAAGCACCAGCCTCGGTCACGTGTAAAGCTGACGCCGCTGCTCTCTGCGTCACGTGCGATTCTGACATCCACTCAGCGAATCCACTCGCCCGCCGCCACGAGCGTGTCCCCGTCGAGCCTTTCTTCGACTCTGCCGAATCCGTCGTGAAATCCTCCGCTGCTGCTGCTGCTTCCTTCAATTTCGTCGTACCTACCGACGACGGTTTCAGCCAAGACGACGCCGAAGCATCTACGTGGTTGATTCCGAATCTGAATCCGAATTTCGGATCTAAACTAACCGAAACTCCAGATATCAAAAACCAGAGATATGTTCTTCTCCGATATGGACTTCCGTTTCTCGTATTTCGAATTACTCTAACAACTTCCAAGAACAATATTGCAACGCAATAAACGATAGCGTTACGG
TARGET=549,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00966 Ps_contig_mira-and-tgicl-ass_franssen_9196
SEQUENCE=AGGGCACTGTCTCTCACTAAACACATCCCCCTTTTCTTCTCTCTCTCCTCTATCCACCCACCACTTCTCTTCACTCCATTTCTCTCTCATCAAATTTCCCCCAATTTTCTCTCCCTCAATTCCCACTCATCACCAATGCAGAATCATCACCGCCATCGCGCTTTCGATCTATAATAATCACCTTTCTCTCTTCCTCTATCTCTTAATTTCAATTCTCCTTTTCAGAAAAATGTTGGAAGCAATGGAAAGTTCAGTGAACGGTGGTTTCTCGCAACATTTACAAAGCTTCGGTGATAGTAGTGAAGAAGAACTCTCAGTTCTTCCACGTCACACCAAAGTCGTTGTAACTGGAAACAATCGCACCAAATCGGTACTCGTTGGTCTTCGTGGTGTTGTCAAGAAAGCTGTTGGACTTGGAGGTTGGCATTGGCTGGTTCTTACCAATGGTATTGAAGTGAAGCTGCAGAGGAATGCTCTTAGTGTGATTGAACCACCTACCGGTAACGAGGTAGATGATGACCTTGAATTTGAGAATACTCAGTGGCATGGATCGGATATGGCATCTGATGAAACTCAGAAGTCCCACAAATCAAGGCATAGGATGCATAGATCATTAGGATCATCTCACAAGAGCATGAGCAGGTCCTTTTCTGTTGATTCACAATCCAAGGGATCCATTTCCATGCCTCATGGGTGGACGAAGGTTGATTTGAGCAAATTGGAAATGGCTGCATTGTGGAGATATTGGCGACACTTCAATCTGGTGGATGCTGTTCCAAACCCGTCCAAAGAGCAGCTGGTAGATGTTGTTCAGAGGCATTTCATGTCACAGCAAATGGATGAGTTGCAAGTTATTCGAGGATTTGTGAAAGCTGCAAAGAGGTTGAAGACAACTATGTGCAAATGATGGAGCTGAGCTGAGCAAAATGCTGATGTTTATGAAGTTATATATTCTAATGCTAACAAAGATAATGGTGTCTACTATGAATATCCATTTCCCTTGGTACTATGTAACATATTTAGCCGATACTAAGTAGTAAGTATCTTTCGGATCTCAATTAGTAGTAGGTTGATTAAGAGGTTATATATAGAATGACTCTTTTGTAGCATCACCTAGTTATATTATGTAGATATGTGACCAAGAACTTGAATCTTGAGTGAAGTTTTAAGGCTTCCTTTAGCTTTTGATTATAATGTGCTGCACCCATGGGAAGTGGGTTTCCTTATCCTTGTGGTTGTATATATCTCTTATTTTCCTTT
TARGET=38,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1752
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.039178
PRIMER_LEFT_SEQUENCE=TCTCACTAAACACATCCCCCTT
PRIMER_RIGHT_SEQUENCE=TGTAAATGTTGCGAGAAACCAC
PRIMER_LEFT=11,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.2968
PRIMER_LEFT_1_PENALTY=0.258908
PRIMER_RIGHT_1_PENALTY=0.037936
PRIMER_LEFT_1_SEQUENCE=CACTAAACACATCCCCCTTTTC
PRIMER_RIGHT_1_SEQUENCE=ATGATTCTGCATTGGTGATGAG
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=146,22
PRIMER_LEFT_1_TM=59.741
PRIMER_RIGHT_1_TM=59.962
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=0.2968
PRIMER_LEFT_2_PENALTY=0.258908
PRIMER_RIGHT_2_PENALTY=0.037936
PRIMER_LEFT_2_SEQUENCE=TCACTAAACACATCCCCCTTTT
PRIMER_RIGHT_2_SEQUENCE=ATGATTCTGCATTGGTGATGAG
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=146,22
PRIMER_LEFT_2_TM=59.741
PRIMER_RIGHT_2_TM=59.962
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=0.2981
PRIMER_LEFT_3_PENALTY=0.258908
PRIMER_RIGHT_3_PENALTY=0.039178
PRIMER_LEFT_3_SEQUENCE=CACTAAACACATCCCCCTTTTC
PRIMER_RIGHT_3_SEQUENCE=TGTAAATGTTGCGAGAAACCAC
PRIMER_LEFT_3=14,22
PRIMER_RIGHT_3=281,22
PRIMER_LEFT_3_TM=59.741
PRIMER_RIGHT_3_TM=60.039
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.2981
PRIMER_LEFT_4_PENALTY=0.258908
PRIMER_RIGHT_4_PENALTY=0.039178
PRIMER_LEFT_4_SEQUENCE=TCACTAAACACATCCCCCTTTT
PRIMER_RIGHT_4_SEQUENCE=TGTAAATGTTGCGAGAAACCAC
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=281,22
PRIMER_LEFT_4_TM=59.741
PRIMER_RIGHT_4_TM=60.039
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_00967 Ps_contig_mira-and-tgicl-ass_franssen_9218
SEQUENCE=GGAGYTAAGAGAAAGAAAAGAATGCGGAAACAAGGAGGAAAGTACACTTCACAAAAACAAACAACCACATCTTCAACTCCGGTTCTTGTTTTTCTCATTTTCCGACCAAACCTGTTCCAAATTCGTCATTCTCCGGCAATGTCTATCAACACCGAATCTTCGCCGCCGCCTCCGGTCATCGGTAAAATTGGACCGTACACTGTTTTCATGACGCCGCCATCAACCCCTAAACCCTCCTCTTCCGAACCGGTTACTACTCTTCCTTCACCAAACATCAACAATGTTAACAATCATGCTAAGATCCTTCCTCCTCCACCTCAGATTCATAACCCTCTCCCTTCTTCCAAAACCCTCTCCTCTGATGCTTCGTCGTTTCTCGGATTTTTCAAGATCGCTGTTAACAGGGTTCAGACCGCTCATTCTAATTTGGATGACCATTTGGCTCGTTGGTTTGGGTTGAATCAGTCAAAGTATCAATGGGCTCTTGATGATTATTACGAGACCAACGGAACTGCAAAAGGAGATGTAAAAGTGAAAGAAGTATCAAGCAAAGTACAGAGTGTTTGACCTGCGTAGTTCTCATGGATTTAATCAAAAGTGAGATGCGAAGAGTCTTGCTTTGAGTATATTTATACCTTCGTCGTATTTACATGTGGAGTAGACAAACAACAATTCTTGTGATATGTTCAAGAGTTATTTGATCTACAACCATTGATCTTCTGATTCAATGCTTCAATGCTTTGTTGTTCTTGTATTGTATTGTATTGTAGTTTAAGAAGTGTTTCAATTTTCCTTGCCTAGGAGCTGTTTTTGTATTTGCATTTGGTTAAGGAAAAACATTGGTACTTGCATTCTTATGGCGACGACAACAACGTTATTATCTCCAAAACAGTTTGTATGACTTAAGAAATGAAAGGGTCTGATAATTTTATTGTTTTGTGTCAAATTTGATAAGATTTGAGCAGAGGTGTATGCATTCTGTCAAAAGAAAACAATAGTGTATGTGATATGGTGAGATATGTAGTCAGATAATGAAGTCTTTTAATAATGTGATTATAGATABTATTACC
TARGET=750,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00968 Ps_contig_mira-and-tgicl-ass_franssen_9227
SEQUENCE=GATTTTTCTTTAATATAAAAAAGAAAAAGGTTTCCGGTTTGCAGTCCGGAATTTTCAGGTCTCTCCCCAAACATCTGCCTAAACGCCATCCCCAAACGCAAAATGACAACCTCTTTCCCTCGCTTGCTCTCCACCGCATCCAACCCTACTCTCCTCCGCACCGGATCCGAACACCTGACTCGTTCTGTTTCCAACTCAGTGACACGCCTCCTCTTCTCCTCCTCGACTCAGCACCACCACCACAACAACACCGTTAGGGATCAACCACAGACACCGGCGCCAGAATCTCTTAAACAAAGCGAAAAGGAACAAGAAGAAGAAGATGACGGCGACGAAATTGATCTGAACAAAGAAACGGGCGAGGTCGGTGGACCCAAAGGGCCTGAGCCCACAAGGTATGGCGATTGGGAACGCAATGGTCGCTGTTCTGATTTTTGATATTTTTCTTCACTTTACCATTAGCGTTACCATAATATAAGTAATAATTTGTGTATTTCTCGTTCAATATTTCAAGATTCACTGCGAATTCTTTTGGTTCTTTACTGTTTTATATATTTATATGTAACTGAGATTGGAACAAATCAATTTGTTGCTTGTATGATCTCTAGAAATAAATTAGTGGTTTGTGATTAACTTGAGGAATGTTGAACAAATTAGTGATATTGATGAGTTAGATGTTGGTTTG
TARGET=232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3267
PRIMER_LEFT_PENALTY=0.325919
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=TCGTTCTGTTTCCAACTCAGTG
PRIMER_RIGHT_SEQUENCE=TCTTCTTCTTGTTCCTTTTCGC
PRIMER_LEFT=179,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=60.326
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.4341
PRIMER_LEFT_1_PENALTY=0.325919
PRIMER_RIGHT_1_PENALTY=0.108206
PRIMER_LEFT_1_SEQUENCE=TCGTTCTGTTTCCAACTCAGTG
PRIMER_RIGHT_1_SEQUENCE=GCCGTCATCTTCTTCTTCTTGT
PRIMER_LEFT_1=179,22
PRIMER_RIGHT_1=329,22
PRIMER_LEFT_1_TM=60.326
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=0.5013
PRIMER_LEFT_2_PENALTY=0.500551
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=CAAAATGACAACCTCTTTCCCT
PRIMER_RIGHT_2_SEQUENCE=TCTTCTTCTTGTTCCTTTTCGC
PRIMER_LEFT_2=98,22
PRIMER_RIGHT_2=319,22
PRIMER_LEFT_2_TM=59.499
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.5230
PRIMER_LEFT_3_PENALTY=0.522246
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=ACGCAAAATGACAACCTCTTTC
PRIMER_RIGHT_3_SEQUENCE=TCTTCTTCTTGTTCCTTTTCGC
PRIMER_LEFT_3=95,22
PRIMER_RIGHT_3=319,22
PRIMER_LEFT_3_TM=60.522
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=0.5859
PRIMER_LEFT_4_PENALTY=0.585203
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=ACACCTGACTCGTTCTGTTTCC
PRIMER_RIGHT_4_SEQUENCE=TCTTCTTCTTGTTCCTTTTCGC
PRIMER_LEFT_4=170,22
PRIMER_RIGHT_4=319,22
PRIMER_LEFT_4_TM=60.585
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_00969 Ps_contig_mira-and-tgicl-ass_franssen_9227
SEQUENCE=GATTTTTCTTTAATATAAAAAAGAAAAAGGTTTCCGGTTTGCAGTCCGGAATTTTCAGGTCTCTCCCCAAACATCTGCCTAAACGCCATCCCCAAACGCAAAATGACAACCTCTTTCCCTCGCTTGCTCTCCACCGCATCCAACCCTACTCTCCTCCGCACCGGATCCGAACACCTGACTCGTTCTGTTTCCAACTCAGTGACACGCCTCCTCTTCTCCTCCTCGACTCAGCACCACCACCACAACAACACCGTTAGGGATCAACCACAGACACCGGCGCCAGAATCTCTTAAACAAAGCGAAAAGGAACAAGAAGAAGAAGATGACGGCGACGAAATTGATCTGAACAAAGAAACGGGCGAGGTCGGTGGACCCAAAGGGCCTGAGCCCACAAGGTATGGCGATTGGGAACGCAATGGTCGCTGTTCTGATTTTTGATATTTTTCTTCACTTTACCATTAGCGTTACCATAATATAAGTAATAATTTGTGTATTTCTCGTTCAATATTTCAAGATTCACTGCGAATTCTTTTGGTTCTTTACTGTTTTATATATTTATATGTAACTGAGATTGGAACAAATCAATTTGTTGCTTGTATGATCTCTAGAAATAAATTAGTGGTTTGTGATTAACTTGAGGAATGTTGAACAAATTAGTGATATTGATGAGTTAGATGTTGGTTTG
TARGET=311,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6423
PRIMER_LEFT_PENALTY=0.151106
PRIMER_RIGHT_PENALTY=0.491155
PRIMER_LEFT_SEQUENCE=AACAACACCGTTAGGGATCAAC
PRIMER_RIGHT_SEQUENCE=ATCAAAAATCAGAACAGCGACC
PRIMER_LEFT=243,22
PRIMER_RIGHT=438,22
PRIMER_LEFT_TM=60.151
PRIMER_RIGHT_TM=60.491
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.7931
PRIMER_LEFT_1_PENALTY=0.151106
PRIMER_RIGHT_1_PENALTY=0.642030
PRIMER_LEFT_1_SEQUENCE=AACAACACCGTTAGGGATCAAC
PRIMER_RIGHT_1_SEQUENCE=AGAACCAAAAGAATTCGCAGTG
PRIMER_LEFT_1=243,22
PRIMER_RIGHT_1=538,22
PRIMER_LEFT_1_TM=60.151
PRIMER_RIGHT_1_TM=60.642
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=0.8171
PRIMER_LEFT_2_PENALTY=0.325919
PRIMER_RIGHT_2_PENALTY=0.491155
PRIMER_LEFT_2_SEQUENCE=TCGTTCTGTTTCCAACTCAGTG
PRIMER_RIGHT_2_SEQUENCE=ATCAAAAATCAGAACAGCGACC
PRIMER_LEFT_2=179,22
PRIMER_RIGHT_2=438,22
PRIMER_LEFT_2_TM=60.326
PRIMER_RIGHT_2_TM=60.491
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=1.0764
PRIMER_LEFT_3_PENALTY=0.585203
PRIMER_RIGHT_3_PENALTY=0.491155
PRIMER_LEFT_3_SEQUENCE=ACACCTGACTCGTTCTGTTTCC
PRIMER_RIGHT_3_SEQUENCE=ATCAAAAATCAGAACAGCGACC
PRIMER_LEFT_3=170,22
PRIMER_RIGHT_3=438,22
PRIMER_LEFT_3_TM=60.585
PRIMER_RIGHT_3_TM=60.491
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=1.1144
PRIMER_LEFT_4_PENALTY=0.623261
PRIMER_RIGHT_4_PENALTY=0.491155
PRIMER_LEFT_4_SEQUENCE=GACTCGTTCTGTTTCCAACTCA
PRIMER_RIGHT_4_SEQUENCE=ATCAAAAATCAGAACAGCGACC
PRIMER_LEFT_4=176,22
PRIMER_RIGHT_4=438,22
PRIMER_LEFT_4_TM=59.377
PRIMER_RIGHT_4_TM=60.491
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_00970 Ps_contig_mira-and-tgicl-ass_franssen_9228
SEQUENCE=ACATCCTCAAGAMKYWCAAACCACTAATTTATTTCTAGAGATCATACACGCAACAAATTGATTTGTTTCAATCTCAGTTACATATAAATATATAAAACAGTAAAAAACCAAAAGAATTCGCAGTGAATCTTGAAATATTGAACGAGAAATACACAAATTATTACTTACATTATGGTAACAGTAATGGTAAAGTGAAGAAAAATATCAAAAATCAGAACAGCGACCATTGCGTTCCCAATCGCCATACCTTGTGGGCTCAGGCCCTTTGGGTCCACCGACCTCGCCCGTTTCTTTGTTCAGATCAATTTCGTCGCCGTCATCTTCTTCTTCTTCTTCCTTTTCGCTTTGTTTAAGAGATTCTGGCGCCGGTGTCTGTGGTTGATCCCTAACGGTGTTGTTGTGGTGGTGCTGAGTCGAGGAGGAGAGGAGACGTGTCACTGAGTTGGAAACAGAACGAGTCAGGTGTTCGGATCCGGTGCGGAGGAGAGTAGGGTTGGATGCGGTGGAGAGCAAGCGAGGGAAAGAGGTTGTCATTTTGCGTTTGGGGATGGCGTTTAGGCAGATGTTTGGAGAGAGACCTGAAAATTCGGACTGCCCCCGAC
TARGET=320,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00971 Ps_contig_mira-and-tgicl-ass_franssen_9235
SEQUENCE=GACCGATTCATTTATAAGTGACCGAACCTCCATTTGACGAGTGGTTTTTTATTTCAACAACTTTCCATCCGCCACTCACTCACACACTAACTCATCAACCATGGCCGCCGCTAGTTTAACTTCCCAAACCCTCAGAGTCCCCACACCAACTTCCCTCTCTCTCTCCTCCTCCAAACTTCCTCTCTCCTCCATTTCCCTTCCGTTCCCACCCCCGTCGCTCTCTAGTCGTCTACGTCTCCTCCGACGCCAAACCCACCGTCCTCGTCGCCGAGAAGCTCGGCGAAGCAGGTCTAACTCTTCTCAAAGACTTCGCCAACGTGGATTGCTCCTACAATCTCAGTCCCGAGGAGCTTTGCACTAAGATCTCGCTCTGTGACGCTCTCATCGTCCGGAGTGGGACTAAGGTCTCCCGTGAAGTCTTTGAGTCCTCCGGTGGTAGGCTTAAGGTAGTCGGAAGAGCTGGCGTGGGAATCGATAACGTGGATCTGGCCGCCGCTACTGAACATGGTTGCTTGGTTGTTAATGCTCCCACTGCGAATACTGTTGCCGCTGCGGAGCACGGGATCGCTCTCTTGGCTTCCATGGCTAGAAATGTTGCTCAGGCTGATGCCTCTATTAAAGCTGGGAAGTGGCTGAGAAATAAGTATGTTGGAGTTTCCCTTGTTGGGAAGACACTTGCTGTACTGGGATTTGGGAAGGTTGGTACTGAAGTTGCTAGGCGTGCTAAGGGGCTTGGTATGAGTGTTATTGCTCATGATCCTTATGCTCCTGCTGATCGTGCACGTGCTATTGGTGTGGAGCTTGTGAGCTTTGATGTGGCCATTGCTACTGCTGATTTCATATCTTTGCATATGCCGCTTACGCCTGCTACCTCGAAGATGCTCAATGACGAGACTTTTGCTAAGATGAAGAAAGGAGTTAGAATTGTCAATGTTGCTCGTGGTGGAGTCATTGATGAGGATGCGCTTGTTAGAGCATTGGATTCTGGAATTGTAGCTCAGGCGGCTCTTGATGTTTTCACGGTGGAACCACCCGCCAAAGACAGCAAGTTGGTTTTACATGAGCTAGTTACTGCGACACCTCATCTAGGTGCCAGTACAACGGAAGCTCAGGAAGGTGTGGCTATTGAAATAGCAGAAGCTGTTGTTGGGGCGTTGAAAGGGGAGCTTGCTGCTACCGCAGTCAATGCACCAATGGTTCCCTCAGAGGTGTTAACTGAGCTAAAACCATTTGTTGTTCTTGCTGAGAAACTGGGTAGGCTGGCTGTCCAACTGGTAGCAGGAGGAAGTGGTGTAAAATCAGTGAAAGTGACTTATGCTTCTGCTAGGGCCCCGGATGATCTTGACACCCGTCTTCTTCGTGCTATGATAACAAAGGGTCTGATAGAGCCCATATCCAGTGTTTTTGTGAACTTAGTTAATGCCGACTTCACTGCTAAACAAAGAGGGCTAAGATTAACCGAGGAGAGAGTTATTCTTGATGGTTCACCTGAGAATCCACTTGAATTCATCCAGGTCCAGATTGCTAATGTGGAAACTCGGTTTGCCGGTGCCATATCAGATTCAGGGGAGGTTACAGTTGAGGGTCGCGTGAAAGATGGTGTCCCTCATCTTACGAAGGTTGGATCTTTTGAAGTTGATGTAAGTTTGGAAGGTAGCATCATACTTTGCAGACAGGTAGATCAACCAGGTATGATTGGAAAGGTGGGAAGCATCTTGGGTGAAGAGAATGTGAATGTCAGCTTCATGAGTGTTGCAAGGACTGCTCCAAGAAAGCATGCTGTGATGGCAATTGGAGTTGATGAACAACCCCGCAAAGAAAGTTTGAAAAAGATTGGAGATATTCCTGCTATCGAAGAATTTGTTTTCCTCAAGTTTTAAGATTAATGTTTTTGTCTTGTGTTTTATTTTTCTTTCAATTGGGCATATCTGGTGTGCCCAGTTTTGATGTATCATCATGAACAAGTGAGTAGTGAGTCTTCGGTAGATGAGGAAAAGAATAATTTAGTATAGTGAAATTCCTGCTTTTTCATTTTCATTTGAAAATAGAGAAAAACAATATGTTGCAAGTTTCAACG
TARGET=155,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2354
PRIMER_LEFT_PENALTY=0.026163
PRIMER_RIGHT_PENALTY=0.209271
PRIMER_LEFT_SEQUENCE=TTTAACTTCCCAAACCCTCAGA
PRIMER_RIGHT_SEQUENCE=CAGAGCGAGATCTTAGTGCAAA
PRIMER_LEFT=114,22
PRIMER_RIGHT=372,22
PRIMER_LEFT_TM=59.974
PRIMER_RIGHT_TM=59.791
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=0.2399
PRIMER_LEFT_1_PENALTY=0.026163
PRIMER_RIGHT_1_PENALTY=0.213725
PRIMER_LEFT_1_SEQUENCE=TTTAACTTCCCAAACCCTCAGA
PRIMER_RIGHT_1_SEQUENCE=TCACAGAGCGAGATCTTAGTGC
PRIMER_LEFT_1=114,22
PRIMER_RIGHT_1=375,22
PRIMER_LEFT_1_TM=59.974
PRIMER_RIGHT_1_TM=59.786
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.2862
PRIMER_LEFT_2_PENALTY=0.026163
PRIMER_RIGHT_2_PENALTY=0.260060
PRIMER_LEFT_2_SEQUENCE=TTTAACTTCCCAAACCCTCAGA
PRIMER_RIGHT_2_SEQUENCE=CTCCTCGGGACTGAGATTGTAG
PRIMER_LEFT_2=114,22
PRIMER_RIGHT_2=349,22
PRIMER_LEFT_2_TM=59.974
PRIMER_RIGHT_2_TM=60.260
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.4067
PRIMER_LEFT_3_PENALTY=0.026163
PRIMER_RIGHT_3_PENALTY=0.380537
PRIMER_LEFT_3_SEQUENCE=TTTAACTTCCCAAACCCTCAGA
PRIMER_RIGHT_3_SEQUENCE=GATTGTAGGAGCAATCCACGTT
PRIMER_LEFT_3=114,22
PRIMER_RIGHT_3=335,22
PRIMER_LEFT_3_TM=59.974
PRIMER_RIGHT_3_TM=60.381
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.4593
PRIMER_LEFT_4_PENALTY=0.384647
PRIMER_RIGHT_4_PENALTY=0.074695
PRIMER_LEFT_4_SEQUENCE=CTCCATTTGACGAGTGGTTTTT
PRIMER_RIGHT_4_SEQUENCE=GGAAATGGAGGAGAGAGGAAGT
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=195,22
PRIMER_LEFT_4_TM=60.385
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=169
=
PRIMER_SEQUENCE_ID=SSR_00972 Ps_contig_mira-and-tgicl-ass_franssen_9254
SEQUENCE=TATTTTCTTCTAATTCACATGTAATCTTTTATAATAATTTTTGAAAGTATAATTATTCAGAAAATTGGTTATCTCCTTATAGATCTGATTAGTAGATTTGTTTAGTCAATTTGGATAAACATTTTCTTTGTGAACTCTATTTTATCTTCATCTTTTTTTGTTATAAATTTGTTTAAATTGTTTGACATATTATTGTTTGAGCTTATTCATTTAAGTTGTCATTTGTTTGTTACACACACATTTTAAATATGAACGAAATTAATATTATTATTGAACTTTTATAAGAAGATTTGAGTGTTGTTAAACCTTTCAATTTCACAACACATTCAAATATAATTGAGTATGATATCATATTACAATTAAATTAAATTAAAAAATAAAAATAAAAATGTTTAAAATTAT
TARGET=358,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_00973 Ps_contig_mira-and-tgicl-ass_franssen_9256
SEQUENCE=TTAAAACAAGCTKCCTCGCGTTCCAACATACTCTCTCTCTTGGGGATCTTGGGTCGTCGACTCCGCCGCGCCAGAAATCGTCTCTGGCGGCGGCGGAGGCGGAGGCGGAGGCTACAAACACTCCAATTCATACCTNATTCTTCGTCCCTCTTCTTTCTTCTCTATACCTAAACCCTAACCTTACTTTCCCATTCCTAA
TARGET=31,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00974 Ps_contig_mira-and-tgicl-ass_franssen_9257
SEQUENCE=AAACAAGAAAGGCAGTTCTACATTTTATTTAACCCGGGCTTGGCAAAACTCAAAAACACTAGTTCAAAGTAGCAGAAACAAATGAACATCAGAATAAATAATTAATTACCACCAGACTGTACATTTCACTTCAATAAAACCATAACTAAGAGCTAACCTTTCCCCACCCAATAAAAATCCAAATAAGAACTATGAACAACACTCAAATCTTACCCTAACACCACCAAAATCCAAGACTACCTCTTGCTTTCCTTTCCTTACGCTAAATTATTTTACTTTATTGTTTTTCTCCTTATTTCGCCCCTTTGGCTTACCAACTGCCATCCAAAATTGCCTAAAAGGTAAAATTGAACATTCACTTCTCTTTTATTCTGTGCATTTGCTTTGCTTTGCTTTAGGACCTTGAAAAAACAAACAAGCGTCCTAAGTCATCACTGTGTGTTTGCTGCTGCCCTGGTGCTTGTTGTTGAGTCTCATGTTGCCTCTGGACAAATTCATCCTGATTCCCATCAATATTCTGGTCTTCTTTGGTGAGGTAGCTGTCAACTTTGGAAGCAGCTTGTCCTTCCCTCTGAAATACGCCCCCGTGTA
TARGET=379,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0666
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.046801
PRIMER_LEFT_SEQUENCE=TACCCTAACACCACCAAAATCC
PRIMER_RIGHT_SEQUENCE=GAGACTCAACAACAAGCACCAG
PRIMER_LEFT=210,22
PRIMER_RIGHT=474,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=59.953
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.0839
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.064105
PRIMER_LEFT_1_SEQUENCE=TACCCTAACACCACCAAAATCC
PRIMER_RIGHT_1_SEQUENCE=CAGTGATGACTTAGGACGCTTG
PRIMER_LEFT_1=210,22
PRIMER_RIGHT_1=436,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=59.936
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=227
PRIMER_PAIR_PENALTY_2=0.1058
PRIMER_LEFT_2_PENALTY=0.019805
PRIMER_RIGHT_2_PENALTY=0.085951
PRIMER_LEFT_2_SEQUENCE=TACCCTAACACCACCAAAATCC
PRIMER_RIGHT_2_SEQUENCE=AGAGGCAACATGAGACTCAACA
PRIMER_LEFT_2=210,22
PRIMER_RIGHT_2=485,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.1058
PRIMER_LEFT_3_PENALTY=0.019805
PRIMER_RIGHT_3_PENALTY=0.085951
PRIMER_LEFT_3_SEQUENCE=TACCCTAACACCACCAAAATCC
PRIMER_RIGHT_3_SEQUENCE=CAGAGGCAACATGAGACTCAAC
PRIMER_LEFT_3=210,22
PRIMER_RIGHT_3=486,22
PRIMER_LEFT_3_TM=59.980
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.2738
PRIMER_LEFT_4_PENALTY=0.226971
PRIMER_RIGHT_4_PENALTY=0.046801
PRIMER_LEFT_4_SEQUENCE=AACACCACCAAAATCCAAGACT
PRIMER_RIGHT_4_SEQUENCE=GAGACTCAACAACAAGCACCAG
PRIMER_LEFT_4=216,22
PRIMER_RIGHT_4=474,22
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=59.953
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=259
=
PRIMER_SEQUENCE_ID=SSR_00975 Ps_contig_mira-and-tgicl-ass_franssen_9262
SEQUENCE=TTGAAGCACTGAAAATTACAAGCTGGCATGGCCGAGGAAGAAGCTCCATTTGATTTTTCTGCACTCAGCTCAAAGAAAGGGGTTAGCGGGAACGATCTTTTGGCTTCTGCTATTCCACTTGAGGATTGGATTAGAAACCAAAGCTTAGGCAACAACAAGGGCTCTGCAACTGCAAGTTCTGATGGCGAAACTGACAGAGTCACACTGATATTGGTTGTCCAACTGCGGGATCCAATGAGACGCTATGAAACAGTTGGAGGCCCTGTTATGGTGCTTATTCATGCATCGCGTGTTGGCGCGAACGGGACTGAGGAGGAGGAGAAAGAAATTCAAACTAATAAGCATGCATGTGGGAGGTTTCAAGGTTAGGAGTTCTACAAAGAAGAACGGATGGGACAACGAGAAGCAGAGACTAACCGCAATGCAATGGTTAGTTGCATACGGGTTGGGAAAAGCGGGGAAGAAAGGGAAACCGGCAGTGGCAAAGGGACAAGACCTGCTATATAGTATTTCATCGCGGATCGTGGCTGATATGTGGCTCAAAACTATTAGGAACCCGGATGTTAAGCTTGTAAAGTGATTAGTTACGCCAAGGAAACAGTCTTAGACATTCATGTATCATAGAAAGTTCTATACAAAGTAGGGTCGGAGA
TARGET=310,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2415
PRIMER_LEFT_PENALTY=0.115779
PRIMER_RIGHT_PENALTY=0.125755
PRIMER_LEFT_SEQUENCE=CGATCTTTTGGCTTCTGCTATT
PRIMER_RIGHT_SEQUENCE=ACTCCTAACCTTGAAACCTCCC
PRIMER_LEFT=92,22
PRIMER_RIGHT=372,22
PRIMER_LEFT_TM=59.884
PRIMER_RIGHT_TM=59.874
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.3571
PRIMER_LEFT_1_PENALTY=0.231323
PRIMER_RIGHT_1_PENALTY=0.125755
PRIMER_LEFT_1_SEQUENCE=CCAATGAGACGCTATGAAACAG
PRIMER_RIGHT_1_SEQUENCE=ACTCCTAACCTTGAAACCTCCC
PRIMER_LEFT_1=231,22
PRIMER_RIGHT_1=372,22
PRIMER_LEFT_1_TM=59.769
PRIMER_RIGHT_1_TM=59.874
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=142
PRIMER_PAIR_PENALTY_2=0.3796
PRIMER_LEFT_2_PENALTY=0.304841
PRIMER_RIGHT_2_PENALTY=0.074807
PRIMER_LEFT_2_SEQUENCE=TGAGACGCTATGAAACAGTTGG
PRIMER_RIGHT_2_SEQUENCE=CGGTTAGTCTCTGCTTCTCGTT
PRIMER_LEFT_2=235,22
PRIMER_RIGHT_2=418,22
PRIMER_LEFT_2_TM=60.305
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=0.3823
PRIMER_LEFT_3_PENALTY=0.256503
PRIMER_RIGHT_3_PENALTY=0.125755
PRIMER_LEFT_3_SEQUENCE=TTTGGCTTCTGCTATTCCACTT
PRIMER_RIGHT_3_SEQUENCE=ACTCCTAACCTTGAAACCTCCC
PRIMER_LEFT_3=98,22
PRIMER_RIGHT_3=372,22
PRIMER_LEFT_3_TM=60.257
PRIMER_RIGHT_3_TM=59.874
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=0.3823
PRIMER_LEFT_4_PENALTY=0.256503
PRIMER_RIGHT_4_PENALTY=0.125755
PRIMER_LEFT_4_SEQUENCE=CTTTTGGCTTCTGCTATTCCAC
PRIMER_RIGHT_4_SEQUENCE=ACTCCTAACCTTGAAACCTCCC
PRIMER_LEFT_4=96,22
PRIMER_RIGHT_4=372,22
PRIMER_LEFT_4_TM=60.257
PRIMER_RIGHT_4_TM=59.874
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=277
=
PRIMER_SEQUENCE_ID=SSR_00976 Ps_contig_mira-and-tgicl-ass_franssen_9273
SEQUENCE=CAGTTTSMSATAAGTTTATTCTCCYTTTTTAAATCTCCTCGTCTCCCTCTATTCTTCTTTCTTCTTTCTAGATTTTGAATGTTGTTTGTTGTTCTTGGTTCGAATGTTACGGTCTTTGTGTTTTACAAAATATTTTTGGTTGTTCTGCTCGGATTCTCAAAAACGTGTATTTTGTTGTTTGGGATTTGATGTTGACAAAGAAATATTTTGATAACTTCTTGAATTTTTCAGTCGTGTTACTGTTGATTTGTTGTTGTTGGTTTGATGTTAACAAAAATATTTTGATAATTTCTTCTTAGATTTTCCTAAAAAAAAACCCTTTTTGTTACCGTTAATTTATTGTTGTTATATATATGATGAATGTTGAGGTCCTGAGATAACTTGTTAAGTTATCCTTTTTCCAGATTTTGTGGGATATTTACTCTGTGGATTTAGAGCTGTTTGGATTGGATATAAGAATGTGTTCTTGGGTGTTTACCTTGATTCATTGTGCAAAAAATTAATTTGTTCCTCTTATCAATTTATTTTTCAGTTTCCCATAAGTGCTTATTTGATAAGTTTTTAAATCTTTGAATTGTTTTTGCAGGTTTTTACAAAAGTGAAGTGATGTTGATTGAAAGTAGTTTATGCGCCAGCTTTGGAGGCTAGGATTGGGGCGCTGTGAATTTCAAACAATTGGCTTTGGAAATGTCTTTGAAGCTTGCTGCTTCAAACTGTTGTATTTTACAGCTTATTTCCAACTTTTTTCAACTTTATTTGATGTAACAAAACTTTTGGATATTTGGATTAATTATGTAACTCGTAGAGTTTTAATTTGAAACTTCTTTTAGGATAATTATGTAACTATTTTGGATTATTTGGAATTTGGAATTAATTATGTATAG
TARGET=248,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00977 Ps_contig_mira-and-tgicl-ass_franssen_9286
SEQUENCE=CAAATATTACAATCAAAGCAACCATTTTCACCACTAGAGATTGTTCTCTCTTCTTCTTCTTCTTCTTCTGTAGTAGATTTACATTTTTGCTTTAGTGAGACTTTCACATTAGAACCAAAGCATGTAGATTCCATTGAATTTTCTACTACAGAAATATACCCTTTGGAAATAAAGAAATTAAGACGTTAGCTTAGCTTGAAAGGAAGAGAAAAGGAAAAAAGTAGAAAGGTGGGAGAGAATTGGATTGAGAAGAGCGCAATGGAGGAGAGTAAATTGAGGATGAAGAAGAAGAAGAAGATTGTGTTGTTAAGGAAACGAACTCCCGAMAATC
TARGET=49,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00978 Ps_contig_mira-and-tgicl-ass_franssen_9286
SEQUENCE=CAAATATTACAATCAAAGCAACCATTTTCACCACTAGAGATTGTTCTCTCTTCTTCTTCTTCTTCTTCTGTAGTAGATTTACATTTTTGCTTTAGTGAGACTTTCACATTAGAACCAAAGCATGTAGATTCCATTGAATTTTCTACTACAGAAATATACCCTTTGGAAATAAAGAAATTAAGACGTTAGCTTAGCTTGAAAGGAAGAGAAAAGGAAAAAAGTAGAAAGGTGGGAGAGAATTGGATTGAGAAGAGCGCAATGGAGGAGAGTAAATTGAGGATGAAGAAGAAGAAGAAGATTGTGTTGTTAAGGAAACGAACTCCCGAMAATC
TARGET=282,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00979 Ps_contig_mira-and-tgicl-ass_franssen_9287
SEQUENCE=GAAAATAAAATAAAATAAAATAAAGTAAATAACVVGGGGAAAGAAGAGGAAGAGGATTGAAGTTAATCCCAGAAAAAGGTTTTCCGAGAGAGTCGAAATGGACGCCGCCGTCACCGCCACCGCGCCGCCGACGAGAATAACCGAAGATAAAGAAGATATGAAACCCTGGGAGCAGCATTCCGGCGTAATCAGACTCCCTCGCTTCGACTACAATGCTCCGTCTTCTCTTCTCACTCACTCTCATTCCGCCTTTCTCATCACCTGTACAATCAAGCGAGAGAAAAGCGCTACTAAAGAAGCTATTACAATCCTTCATAGCTTCATCAATGGCGATCAACACTCTTCCGACAAAACTGATACTACTTCCAAGAGAAGGAAAATATCCGCACCAGAAACTGCTGTCAAAGAGGAAGGAGTTACCGATCTTTCTCTGGTTAAACTCATAAGAAATGGATTGGTACTTTTTATTTTTCCTAAAAATACATTTCCAGATACTGTTGGAATTGTATCTAGTGTTATTCAGGCTTTGGAGTCTGGGAAGATAACTTCGCCGGCCTGGTGCCATCGCATTTTGCCCATCCAAGGTACCTGTAGTTTGAATGAAAAAGAACTGACAATGAAGTAGTATCAGTGCTTGTT
TARGET=2,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00980 Ps_contig_mira-and-tgicl-ass_franssen_9288
SEQUENCE=AGCTGGAAATGTTACTTGCAAATTCACTTCTTGCAGTTAATTTATATATATATATGATATAGCCATTTTGCTAGCATGTTTGGGAAATGATTATTTTGAAAAGTTTACTTAGAAATATTTTGAATGAAAATATTGACTATAAAATAAGCTAACCCAAACATGCATGCTAGAGTGTTAATGTAATGATATTATATATAACTTTTTTATGTATTCTATAAAGGAGATTTGGTGTTGTATATATGGTTTTGAATATGTAACAAATGAGACATAGTTAGTCCCATTTTCATAATCATACATACATTACTTCTTATCTYAA
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00981 Ps_contig_mira-and-tgicl-ass_franssen_9297
SEQUENCE=TACGGTTAGGGTATAAAACGGGACGTTTATTATAAACACTCAAGTCTCATTCTGAGGGAGCTTCTTCAACCTTCATCTTTAACTTCTCCATTTCACTCTCTCTGCAAAACCTAACTAGAAAAACGAATGAAAATCCTTCATAGATGCAGAGGAGTTTTTTTTCCATCTTCACTATTCTTCATTTCATTTCATTCTTCAATCACGATTCACTCTACATTGTTCGGTACAAATCTCAGAACAAAAAAAGAATACATCAAAATATGAAGAAATCCAATTTCTTGATTCAAATCAGAGTCCAACAGAAAAGAGAGGAGCAGACGCAGAAGAACGACGTGGAATAGAAGAAAATCTTCGATTTCACTTCAGTCCATATTCAGTTTCAATTTCACACCTTTGACATTTGTTTGGGATAGAACGTTGAACCGAATTGAGGCGGCACCATTGAGTTCTAGAAAATGCGACAACAACGCG
TARGET=178,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5728
PRIMER_LEFT_PENALTY=0.384515
PRIMER_RIGHT_PENALTY=0.188254
PRIMER_LEFT_SEQUENCE=CTCCATTTCACTCTCTCTGCAA
PRIMER_RIGHT_SEQUENCE=CGTCTGCTCCTCTCTTTTCTGT
PRIMER_LEFT=85,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=59.615
PRIMER_RIGHT_TM=60.188
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=0.6534
PRIMER_LEFT_1_PENALTY=0.506150
PRIMER_RIGHT_1_PENALTY=0.147295
PRIMER_LEFT_1_SEQUENCE=CCATTTCACTCTCTCTGCAAAA
PRIMER_RIGHT_1_SEQUENCE=TGGACTGAAGTGAAATCGAAGA
PRIMER_LEFT_1=87,22
PRIMER_RIGHT_1=369,22
PRIMER_LEFT_1_TM=59.494
PRIMER_RIGHT_1_TM=59.853
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.6944
PRIMER_LEFT_2_PENALTY=0.506150
PRIMER_RIGHT_2_PENALTY=0.188254
PRIMER_LEFT_2_SEQUENCE=CCATTTCACTCTCTCTGCAAAA
PRIMER_RIGHT_2_SEQUENCE=CGTCTGCTCCTCTCTTTTCTGT
PRIMER_LEFT_2=87,22
PRIMER_RIGHT_2=319,22
PRIMER_LEFT_2_TM=59.494
PRIMER_RIGHT_2_TM=60.188
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.7936
PRIMER_LEFT_3_PENALTY=0.605368
PRIMER_RIGHT_3_PENALTY=0.188254
PRIMER_LEFT_3_SEQUENCE=AGGGAGCTTCTTCAACCTTCAT
PRIMER_RIGHT_3_SEQUENCE=CGTCTGCTCCTCTCTTTTCTGT
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=319,22
PRIMER_LEFT_3_TM=60.605
PRIMER_RIGHT_3_TM=60.188
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=0.8777
PRIMER_LEFT_4_PENALTY=0.384515
PRIMER_RIGHT_4_PENALTY=0.493142
PRIMER_LEFT_4_SEQUENCE=CTCCATTTCACTCTCTCTGCAA
PRIMER_RIGHT_4_SEQUENCE=GCTCCTCTCTTTTCTGTTGGAC
PRIMER_LEFT_4=85,22
PRIMER_RIGHT_4=314,22
PRIMER_LEFT_4_TM=59.615
PRIMER_RIGHT_4_TM=59.507
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_00982 Ps_contig_mira-and-tgicl-ass_franssen_9299
SEQUENCE=TTATATGCGTGAGCTGCGAGAAGAATGCGACAATGACAATTGCCCTTGGTAGCATGCGAATAACTATGGGGATGGAAACATCAATCATCATCGTCGTATAACAGATCCAGCTTCAAGCCTTGCTGCAAGATAGAAGGTTAAATTGTATTAATCTAAAGCGGAAACTAGAAACTAGATCCAGATCCATCAGTCTCTAACCGAATTATACTAACCAAAATTATGCATTGGATAACCAAGTGATTTTCGTAATACAATTTAAATTCCAGAAACTAGAGTTCCAACTAATCCTAACTAACTTAAAAGTGCCCAAGCTCTAACAAACCTGCCCGATCCATTGCCCGAATCGGTTAGGATAAAGGCTTTATATAAACACTTCAAGTCTCATTCTGAGGGAGCTTCTTCAACCTTCATCTTTAACTTCTCCATTTCACTCTCTCTGCAAAACCAACAGAAAAACGAATGAAAATCCTTCATAGATGCAGAGGAGTTTTTTTCCATCTTCACTATTCTTCATTTCATTTCATTCTTCAATCACGATTCACTCTACATTGTTCGGTAACAAATCTCAGAAACAAAAAAGATCATCAAAATATGAAAGAAATCCTAATTTCTTAGATTA
TARGET=510,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3453
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=1.315001
PRIMER_LEFT_SEQUENCE=TCTCTCTGCAAAACCAACAGAA
PRIMER_RIGHT_SEQUENCE=CGAACAATGTAGAGTGAATCGTG
PRIMER_LEFT=431,22
PRIMER_RIGHT=554,23
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=59.685
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=1.3453
PRIMER_LEFT_1_PENALTY=0.030276
PRIMER_RIGHT_1_PENALTY=1.315001
PRIMER_LEFT_1_SEQUENCE=TTCACTCTCTCTGCAAAACCAA
PRIMER_RIGHT_1_SEQUENCE=CGAACAATGTAGAGTGAATCGTG
PRIMER_LEFT_1=426,22
PRIMER_RIGHT_1=554,23
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=59.685
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=1.3453
PRIMER_LEFT_2_PENALTY=0.030276
PRIMER_RIGHT_2_PENALTY=1.315001
PRIMER_LEFT_2_SEQUENCE=TTTCACTCTCTCTGCAAAACCA
PRIMER_RIGHT_2_SEQUENCE=CGAACAATGTAGAGTGAATCGTG
PRIMER_LEFT_2=425,22
PRIMER_RIGHT_2=554,23
PRIMER_LEFT_2_TM=60.030
PRIMER_RIGHT_2_TM=59.685
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=130
PRIMER_PAIR_PENALTY_3=1.4488
PRIMER_LEFT_3_PENALTY=0.030276
PRIMER_RIGHT_3_PENALTY=1.418512
PRIMER_LEFT_3_SEQUENCE=TCTCTCTGCAAAACCAACAGAA
PRIMER_RIGHT_3_SEQUENCE=CCGAACAATGTAGAGTGAATCGT
PRIMER_LEFT_3=431,22
PRIMER_RIGHT_3=555,23
PRIMER_LEFT_3_TM=60.030
PRIMER_RIGHT_3_TM=60.419
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=1.4488
PRIMER_LEFT_4_PENALTY=0.030276
PRIMER_RIGHT_4_PENALTY=1.418512
PRIMER_LEFT_4_SEQUENCE=TTCACTCTCTCTGCAAAACCAA
PRIMER_RIGHT_4_SEQUENCE=CCGAACAATGTAGAGTGAATCGT
PRIMER_LEFT_4=426,22
PRIMER_RIGHT_4=555,23
PRIMER_LEFT_4_TM=60.030
PRIMER_RIGHT_4_TM=60.419
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.0000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_00983 Ps_contig_mira-and-tgicl-ass_franssen_9305
SEQUENCE=ATCACCAATGAGGGATGAAGGATGATGATGATGATAAAGGAGTTAGAGTTTTTCTTTTGTACAGATTTTGTTGTAATAATTTGGTCTAGAAATTAAAGCAGCTTAATTTTCTGATATATTAATAATTTGTTTTTGTTATATTAGTATTACTTAGTATGTTGTTGTTACTTTCTCTATGGTTCTTTTGGTAGTGGTGGGGGGAAGTGAGAACATAAAAGTTGACTTTTATCATAAGATCATATAATTTGAGHAATTT
TARGET=21,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00984 Ps_contig_mira-and-tgicl-ass_franssen_9314
SEQUENCE=GGAGACACACGAATTTGTTGGGACATTGAAGCTAAAGAAAAACCTCATCTCTATCTCTTCTCCTCGKSGACTCGTCCCTGCAACACCACCACTTCAAACTTCACACCCAGTTTTTTTCTTTCTCTTTTCTTTTTAGCGATGGCTTATATAAAATCTTGTATTTCTTATTCTGCTTTACCCTTCTTCCAACGGAGGGGTCTACCACCTGCCTCTTCTTCACCATTTCATCACACAGGAACATTCATTCCCCAATGTTTAACACCAAAAATATTAAGTGCCAAGTTTTCAAAGAATGTCAGATTCTCTATTCCTAGAAGTACTACTTCTTCTTCTTCAAACTCCACTGATGATGATGA
TARGET=323,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00985 Ps_contig_mira-and-tgicl-ass_franssen_9314
SEQUENCE=GGAGACACACGAATTTGTTGGGACATTGAAGCTAAAGAAAAACCTCATCTCTATCTCTTCTCCTCGKSGACTCGTCCCTGCAACACCACCACTTCAAACTTCACACCCAGTTTTTTTCTTTCTCTTTTCTTTTTAGCGATGGCTTATATAAAATCTTGTATTTCTTATTCTGCTTTACCCTTCTTCCAACGGAGGGGTCTACCACCTGCCTCTTCTTCACCATTTCATCACACAGGAACATTCATTCCCCAATGTTTAACACCAAAAATATTAAGTGCCAAGTTTTCAAAGAATGTCAGATTCTCTATTCCTAGAAGTACTACTTCTTCTTCTTCAAACTCCACTGATGATGATGA
TARGET=345,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_00986 Ps_contig_mira-and-tgicl-ass_franssen_9316
SEQUENCE=TTCTAGATTATTATGATATTAAGTACAAAGTTTGGTTTGGTAAGTTAATCCGATGAACAAGAAAGAGATTAAATGGTCTGATTACAAAAAAGGTGCCTATTGTCACTGTTGATGGTGAACAATTGGTTGATTCTTCAGACATAATTGATAAATTGGTCAAAAGGATACATCCAGATTATGACCTAAATGCTGATGAAGAGAAGAAGTGGCGTGGGTGGGTGGATAATCACTTGGTGNATTGTTTTGTCCGACCAAACATATATACGAACTTGGTTTACGGGAAGTCCTTCTTAG
TARGET=211,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0797
PRIMER_LEFT_PENALTY=0.048588
PRIMER_RIGHT_PENALTY=0.031122
PRIMER_LEFT_SEQUENCE=TGTCACTGTTGATGGTGAACAA
PRIMER_RIGHT_SEQUENCE=GGACAAAACAATNCACCAAGTG
PRIMER_LEFT=100,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=60.049
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.75
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=0.3152
PRIMER_LEFT_1_PENALTY=0.048588
PRIMER_RIGHT_1_PENALTY=0.266650
PRIMER_LEFT_1_SEQUENCE=TGTCACTGTTGATGGTGAACAA
PRIMER_RIGHT_1_SEQUENCE=AAGGACTTCCCGTAAACCAAGT
PRIMER_LEFT_1=100,22
PRIMER_RIGHT_1=288,22
PRIMER_LEFT_1_TM=60.049
PRIMER_RIGHT_1_TM=60.267
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=0.3152
PRIMER_LEFT_2_PENALTY=0.048588
PRIMER_RIGHT_2_PENALTY=0.266650
PRIMER_LEFT_2_SEQUENCE=TGTCACTGTTGATGGTGAACAA
PRIMER_RIGHT_2_SEQUENCE=AGGACTTCCCGTAAACCAAGTT
PRIMER_LEFT_2=100,22
PRIMER_RIGHT_2=287,22
PRIMER_LEFT_2_TM=60.049
PRIMER_RIGHT_2_TM=60.267
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.4355
PRIMER_LEFT_3_PENALTY=0.048588
PRIMER_RIGHT_3_PENALTY=0.386884
PRIMER_LEFT_3_SEQUENCE=TGTCACTGTTGATGGTGAACAA
PRIMER_RIGHT_3_SEQUENCE=CTTCCCGTAAACCAAGTTCGTA
PRIMER_LEFT_3=100,22
PRIMER_RIGHT_3=283,22
PRIMER_LEFT_3_TM=60.049
PRIMER_RIGHT_3_TM=60.387
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.6047
PRIMER_LEFT_4_PENALTY=0.338015
PRIMER_RIGHT_4_PENALTY=0.266650
PRIMER_LEFT_4_SEQUENCE=AGGTGCCTATTGTCACTGTTGA
PRIMER_RIGHT_4_SEQUENCE=AGGACTTCCCGTAAACCAAGTT
PRIMER_LEFT_4=90,22
PRIMER_RIGHT_4=287,22
PRIMER_LEFT_4_TM=59.662
PRIMER_RIGHT_4_TM=60.267
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=198
=
PRIMER_SEQUENCE_ID=SSR_00987 Ps_contig_mira-and-tgicl-ass_franssen_9318
SEQUENCE=CTTAGAACACCGAAGACTGCAAGATCTGCTAAATTAGGTTCCAAATCACCAAGAAACTTTCGGCCCTTTAAAGCATCAACCCATTGTTCAGCAGCTCCATACAACGCTTCACGCTCATCGGTGATATTGTGTTTCTTCTTCAATTTCTTCGACACAAAATACATAGCAGCAGCCCCACCATACTTTGCCACTAACCTCTCATATAAACTGAAATTGCCTTTAGTGGTGATATAATCAAATGATTCAAGAGCTTCCGAAACAGTTCGATATATGTTTGGCGACAAAACATGCACCAAGTGATTATCCACCCACCCACGCCACTTCTTCTCTTCATCAGCATTTAGGTCATAATCTGGATGTATCCTTTTGACCAATTTATCAATTATGTCTGAAGAATCAACCAATTGTTCACCATCAACAGTGACAATAGGCACCTTTTTGTAATCAGACCATTTAATCTCTTTCTTGTTCATCGGATTAACTTCCACCACTTTGTACTTAATATCATAATAATCTAGAAAAGCTTTAACCTTGTTGCAGAAGGGACAAGCTTCATACTGAAAGAGAACAACTTCTTTGGGATTGAAGCGATTTTGTTCTGAGCCTGTTGCTTTGAGATCTTGCTGCGCAAGAGACGTGGCAGTAACAGAACCGTAATACCTGAACCCTACGGCGTTACTTCGAATAGCTAGGGCAGTTGATAGGTCGGAGCGATCAACGAGATTCCGAGGGTTGGAAGAAGTCCAGCATGTTGTGATATATAGAGCTGATCGCGGCGGCCGGAGGAGAGTGGTTACGGCGGTGGAGTTGCCTTTTAGCGCCTTTGCGACGGATATAGCTCCATTGAGACCTCTCATTTTTTTTTGGTGGAAATGGTTCCCCCCGAC
TARGET=305,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0634
PRIMER_LEFT_PENALTY=0.014792
PRIMER_RIGHT_PENALTY=0.048588
PRIMER_LEFT_SEQUENCE=CAGTTCGATATATGTTTGGCGA
PRIMER_RIGHT_SEQUENCE=TGTCACTGTTGATGGTGAACAA
PRIMER_LEFT=259,22
PRIMER_RIGHT=425,22
PRIMER_LEFT_TM=59.985
PRIMER_RIGHT_TM=60.049
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.1374
PRIMER_LEFT_1_PENALTY=0.014792
PRIMER_RIGHT_1_PENALTY=0.122569
PRIMER_LEFT_1_SEQUENCE=CAGTTCGATATATGTTTGGCGA
PRIMER_RIGHT_1_SEQUENCE=GGTCTGATTACAAAAAGGTGCC
PRIMER_LEFT_1=259,22
PRIMER_RIGHT_1=450,22
PRIMER_LEFT_1_TM=59.985
PRIMER_RIGHT_1_TM=59.877
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.1434
PRIMER_LEFT_2_PENALTY=0.014792
PRIMER_RIGHT_2_PENALTY=0.128591
PRIMER_LEFT_2_SEQUENCE=CAGTTCGATATATGTTTGGCGA
PRIMER_RIGHT_2_SEQUENCE=AAAGTGGTGGAAGTTAATCCGA
PRIMER_LEFT_2=259,22
PRIMER_RIGHT_2=493,22
PRIMER_LEFT_2_TM=59.985
PRIMER_RIGHT_2_TM=59.871
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=235
PRIMER_PAIR_PENALTY_3=0.1441
PRIMER_LEFT_3_PENALTY=0.095523
PRIMER_RIGHT_3_PENALTY=0.048588
PRIMER_LEFT_3_SEQUENCE=GACAAAACATGCACCAAGTGAT
PRIMER_RIGHT_3_SEQUENCE=TGTCACTGTTGATGGTGAACAA
PRIMER_LEFT_3=279,22
PRIMER_RIGHT_3=425,22
PRIMER_LEFT_3_TM=59.904
PRIMER_RIGHT_3_TM=60.049
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=0.1701
PRIMER_LEFT_4_PENALTY=0.014792
PRIMER_RIGHT_4_PENALTY=0.155310
PRIMER_LEFT_4_SEQUENCE=CAGTTCGATATATGTTTGGCGA
PRIMER_RIGHT_4_SEQUENCE=CCCTTCTGCAACAAGGTTAAAG
PRIMER_LEFT_4=259,22
PRIMER_RIGHT_4=544,22
PRIMER_LEFT_4_TM=59.985
PRIMER_RIGHT_4_TM=60.155
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_00988 Ps_contig_mira-and-tgicl-ass_franssen_9326
SEQUENCE=TCGGGGATAACCTCTTCATTATAATTTATAACATTTAACATGTCAAACATAGTTTTAAAAGACAAGTACAGATCTATTTTGCATGATGAAGATGAGAACATCCAGTGGAGACATGGTGGCCCTCCCACTTATGGTCTTGTCAACCAGCTTTTCGAACAAGGAAGAACCAAAGAATGGCCAGAAGGATCATTGGAGGAAATAGTGCAAAATGGTATTAAGTCATGGGAGATGGAACTCTCTCACAAGATCCGGTTGCAGGACTTCAAGACCATTGTGCCTGAAAAATTTAAGCTCTTTGTTAATGGCAGAGAGGGACTAAGTGCAGAGGAAACTCTAAGTTTAGGAAGTTATAATGCATTGTTGAAAAGTTCTTTGCCAGAAAATTTGAAGCCATATAATTCAAATGAAGAGACTTTTGAATCCTCTCATGAAGTGTTCAAATCAGCTTTTCCAAGAGGATTTGCATGGGAAGTGATTAAAGTTTACACAGGACCACCTGAAATTGCTTTCAAGTTTAGGCATTGGGGATTCTTTGAAGGTCCATTCAAAGGACATGCCCCTAATGGGAAAATAGTCCAATTCTTTGGCTTGGGAACTCTCAAGGTGGATGATGCTTTGAAGGTTGAAGAGGTGGAAATTTACTATGACCCAGCAGAGTTGCTTGGAGGCCTTCTATCAAATGGAGATAGTACAAAAACTTCACCTTGTCCTTTTTCCAATTAATAAATTATTTACTATAAAATTGCAAGTGAATAATAATAATAATAATAATATTTGATCTTGCTGTATTTCTAGAATAAATTTATGGGGGCAGAGGAATTCATTTATTTTTCTTCTATTTTGTGAATAAAAAATTGTGAGCCGAAGTAATTACTCTAGTATATAGTAAGGTGAATTTCTTTAACTTT
TARGET=752,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5102
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.498050
PRIMER_LEFT_SEQUENCE=ACTTCACCTTGTCCTTTTTCCA
PRIMER_RIGHT_SEQUENCE=AATAAATGAATTCCTCTGCCCC
PRIMER_LEFT=696,22
PRIMER_RIGHT=828,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.498
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=12.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=0.5216
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.498050
PRIMER_LEFT_1_SEQUENCE=TGAAGGTTGAAGAGGTGGAAAT
PRIMER_RIGHT_1_SEQUENCE=AATAAATGAATTCCTCTGCCCC
PRIMER_LEFT_1=616,22
PRIMER_RIGHT_1=828,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.498
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=12.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.5557
PRIMER_LEFT_2_PENALTY=0.057688
PRIMER_RIGHT_2_PENALTY=0.498050
PRIMER_LEFT_2_SEQUENCE=ATGCCCCTAATGGGAAAATAGT
PRIMER_RIGHT_2_SEQUENCE=AATAAATGAATTCCTCTGCCCC
PRIMER_LEFT_2=553,22
PRIMER_RIGHT_2=828,22
PRIMER_LEFT_2_TM=59.942
PRIMER_RIGHT_2_TM=60.498
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=12.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.5867
PRIMER_LEFT_3_PENALTY=0.088662
PRIMER_RIGHT_3_PENALTY=0.498050
PRIMER_LEFT_3_SEQUENCE=CTTGGAGGCCTTCTATCAAATG
PRIMER_RIGHT_3_SEQUENCE=AATAAATGAATTCCTCTGCCCC
PRIMER_LEFT_3=660,22
PRIMER_RIGHT_3=828,22
PRIMER_LEFT_3_TM=60.089
PRIMER_RIGHT_3_TM=60.498
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=12.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=0.6371
PRIMER_LEFT_4_PENALTY=0.139083
PRIMER_RIGHT_4_PENALTY=0.498050
PRIMER_LEFT_4_SEQUENCE=AACTCTCAAGGTGGATGATGCT
PRIMER_RIGHT_4_SEQUENCE=AATAAATGAATTCCTCTGCCCC
PRIMER_LEFT_4=593,22
PRIMER_RIGHT_4=828,22
PRIMER_LEFT_4_TM=60.139
PRIMER_RIGHT_4_TM=60.498
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=12.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_00989 Ps_contig_mira-and-tgicl-ass_franssen_9330
SEQUENCE=CAACGAGGAAGAGATTACTATCTTCAAGAGAATGACACTGCACAACCCTTCTCTTCTACTTCAACGCTTTCACCCGCTGACAACGAAGAAGAAGAAGAAGCAATAGATGACGCTGATTCTGACAACAACAACAATGAACAACACCCATCACACGATATGCCTTCAAAATTTCTCCTTTACCAACAATCTGTCCAGTCTCCCAAAGGAGACATTAGTTATTTGCAAAAGTTCTTTCTCATGTATGTTGGGGGAAGGGTGCCCCTCCATCTTCAAGAAGATTTCTGCGGCACTGCGTTTCTTAGTACAGAATGGCTCCGGAGCGATCCAAGAAAGACAGCCATAGGATTAGATTTGGATCTTGAGGCACTTACT
TARGET=85,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7949
PRIMER_LEFT_PENALTY=0.456883
PRIMER_RIGHT_PENALTY=0.338015
PRIMER_LEFT_SEQUENCE=CCCTTCTCTTCTACTTCAACGC
PRIMER_RIGHT_SEQUENCE=GGGTGTTGTTCATTGTTGTTGT
PRIMER_LEFT=45,22
PRIMER_RIGHT=145,22
PRIMER_LEFT_TM=59.543
PRIMER_RIGHT_TM=59.662
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=0.8358
PRIMER_LEFT_1_PENALTY=0.456883
PRIMER_RIGHT_1_PENALTY=0.378958
PRIMER_LEFT_1_SEQUENCE=CCCTTCTCTTCTACTTCAACGC
PRIMER_RIGHT_1_SEQUENCE=GTGATGGGTGTTGTTCATTGTT
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=150,22
PRIMER_LEFT_1_TM=59.543
PRIMER_RIGHT_1_TM=59.621
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=0.8358
PRIMER_LEFT_2_PENALTY=0.456883
PRIMER_RIGHT_2_PENALTY=0.378958
PRIMER_LEFT_2_SEQUENCE=CCCTTCTCTTCTACTTCAACGC
PRIMER_RIGHT_2_SEQUENCE=GATGGGTGTTGTTCATTGTTGT
PRIMER_LEFT_2=45,22
PRIMER_RIGHT_2=148,22
PRIMER_LEFT_2_TM=59.543
PRIMER_RIGHT_2_TM=59.621
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=0.8420
PRIMER_LEFT_3_PENALTY=0.456883
PRIMER_RIGHT_3_PENALTY=0.385098
PRIMER_LEFT_3_SEQUENCE=CCCTTCTCTTCTACTTCAACGC
PRIMER_RIGHT_3_SEQUENCE=CCTATGGCTGTCTTTCTTGGAT
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=343,22
PRIMER_LEFT_3_TM=59.543
PRIMER_RIGHT_3_TM=59.615
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.8709
PRIMER_LEFT_4_PENALTY=0.456883
PRIMER_RIGHT_4_PENALTY=0.414033
PRIMER_LEFT_4_SEQUENCE=CCCTTCTCTTCTACTTCAACGC
PRIMER_RIGHT_4_SEQUENCE=GGAGACTGGACAGATTGTTGGT
PRIMER_LEFT_4=45,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=59.543
PRIMER_RIGHT_4_TM=60.414
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_00990 Ps_contig_mira-and-tgicl-ass_franssen_9330
SEQUENCE=CAACGAGGAAGAGATTACTATCTTCAAGAGAATGACACTGCACAACCCTTCTCTTCTACTTCAACGCTTTCACCCGCTGACAACGAAGAAGAAGAAGAAGCAATAGATGACGCTGATTCTGACAACAACAACAATGAACAACACCCATCACACGATATGCCTTCAAAATTTCTCCTTTACCAACAATCTGTCCAGTCTCCCAAAGGAGACATTAGTTATTTGCAAAAGTTCTTTCTCATGTATGTTGGGGGAAGGGTGCCCCTCCATCTTCAAGAAGATTTCTGCGGCACTGCGTTTCTTAGTACAGAATGGCTCCGGAGCGATCCAAGAAAGACAGCCATAGGATTAGATTTGGATCTTGAGGCACTTACT
TARGET=122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5116
PRIMER_LEFT_PENALTY=0.126537
PRIMER_RIGHT_PENALTY=0.385098
PRIMER_LEFT_SEQUENCE=GCAATAGATGACGCTGATTCTG
PRIMER_RIGHT_SEQUENCE=ATCCTATGGCTGTCTTTCTTGG
PRIMER_LEFT=99,22
PRIMER_RIGHT=345,22
PRIMER_LEFT_TM=59.873
PRIMER_RIGHT_TM=59.615
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=247
PRIMER_PAIR_PENALTY_1=0.5116
PRIMER_LEFT_1_PENALTY=0.126537
PRIMER_RIGHT_1_PENALTY=0.385098
PRIMER_LEFT_1_SEQUENCE=GCAATAGATGACGCTGATTCTG
PRIMER_RIGHT_1_SEQUENCE=CCTATGGCTGTCTTTCTTGGAT
PRIMER_LEFT_1=99,22
PRIMER_RIGHT_1=343,22
PRIMER_LEFT_1_TM=59.873
PRIMER_RIGHT_1_TM=59.615
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.5406
PRIMER_LEFT_2_PENALTY=0.126537
PRIMER_RIGHT_2_PENALTY=0.414033
PRIMER_LEFT_2_SEQUENCE=GCAATAGATGACGCTGATTCTG
PRIMER_RIGHT_2_SEQUENCE=GGAGACTGGACAGATTGTTGGT
PRIMER_LEFT_2=99,22
PRIMER_RIGHT_2=199,22
PRIMER_LEFT_2_TM=59.873
PRIMER_RIGHT_2_TM=60.414
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=101
PRIMER_PAIR_PENALTY_3=0.7493
PRIMER_LEFT_3_PENALTY=0.126537
PRIMER_RIGHT_3_PENALTY=0.622772
PRIMER_LEFT_3_SEQUENCE=GCAATAGATGACGCTGATTCTG
PRIMER_RIGHT_3_SEQUENCE=CTCCGGAGCCATTCTGTACTAA
PRIMER_LEFT_3=99,22
PRIMER_RIGHT_3=319,22
PRIMER_LEFT_3_TM=59.873
PRIMER_RIGHT_3_TM=60.623
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.7493
PRIMER_LEFT_4_PENALTY=0.126537
PRIMER_RIGHT_4_PENALTY=0.622772
PRIMER_LEFT_4_SEQUENCE=GCAATAGATGACGCTGATTCTG
PRIMER_RIGHT_4_SEQUENCE=TCCGGAGCCATTCTGTACTAAG
PRIMER_LEFT_4=99,22
PRIMER_RIGHT_4=318,22
PRIMER_LEFT_4_TM=59.873
PRIMER_RIGHT_4_TM=60.623
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_00991 Ps_contig_mira-and-tgicl-ass_franssen_9347
SEQUENCE=ACTATTGATACGCATAGCGGGGTAGTTGACTTCCCCTTTCGGAAATCCCACCAAAATAATAATTCCATTTATTGTGGNGCCGAGCATCATGATTACCATCATTATGTTGGAGGACAAAGCCAAAGCAATTTTGCATCAGTTTTCACGTACTGCGATTACATCTATGGAACTGACAAGGGTTTTCGGGTTATCAGAAGAAGATACTTCAAAATCTGAAGGAAGATTTGACAAATGGTGCTGCACAGAATAGAGGATCATACGATGCACAAGATTATAGTAAATCTGAGTGAGACAGACGAATAACTAGTACTAGTTAAATAGTTGTCTTGGGTTAGCTTCAGGATGAAGACTACTAGAATGGAGCTTTAGGAATTGTGGTTTTGTTTTACAAGAATGGGTGGGGGAAGCATTTTTCTGATATCATGTTATGGAAATCTAACATTTGGTTGTGCTTTATGTCGATGTACGAATGATCATTTCCTTGTAAGCTGCAAATTAAATTCTGGAAATATGCAGCACGCTTCGTTCCTCCTTCCGGCGGGTAATTTGGAAAGCAGAATAAAATTTGGAAGTTTAGGTTTWAWRWAAWARMTCTTCATGAACAGGTAATGTCCCTTCTACCATATGTAGTCTATAAACAATGGAAAATCATGATGGATTTAGTCAGGAACTCAGAAGTCAAGTTTTGTGAAACACTATTTTGCTACAATAATTTATTTATTTTTTTCAGTGTGAAATTAACTGAAAACTAAATT
TARGET=712,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00992 Ps_contig_mira-and-tgicl-ass_franssen_9365
SEQUENCE=CTTTTTTTTTTAKATAGTAAACTATATAATATTCTCTTTATATATATTAATCAAAAATACACTCCACAATATCGACATAGAAGTAGACATAATCAAAAGCACATAAGCTCCATAGAAAATTTATCTACTTTCTATTTATTTATCTAAAAGTATATGATCAAAACCATATATTATGAGTCATTGCATACACATAATAATATTCTAGTACTATACTCTAGTACTAGAAAACAACAACTCTTGCAATACATATCTATAATAAATGCACTTTACTCAACAATAAGCACTACACTTGTTCTTGCAATCAATGGTACAACCACCACCACCGCCGCCACCACCGTACCCCTTACCGGAATGGCTGAAAGAAGAGAAACACTTAGCTGGACATGTCAACTTTTTCTGATAACATGGACCTTTCTCAGTGCATACAACAGAAGATCTTATTATACCATTCTTAGAGTGTCCTCCATTCGGACCTCCATACCCGGATCCATATCCACCTCCAAATCCATTACCGAACCCGTTTCCAAAACCCGGAATATTAAAACCTCCTCCAGGTCCAAAAACACCACCTGGTATACCACCACCTTGGTTGTTATTGTTGTTGTCCTTAGGGTTAGGGTTAGAGTGTTTGGTTTGAGTAGAAGGAGAAGATCCAGATGAAACGGATCGGGTTGCAAAAGAGGATGAAGCTATGAGAATAAGAGTAGTGAACAAGAGAAAAGTGGTCATAGGTTTCATTTTGTTTTGTTTTTTGTGTGTGTTTGGTTGAGTAAGAATACGATCTGAAATACTATCCGCGTACKAACCCACTTGTCCTTTCGTAGG
TARGET=313,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00993 Ps_contig_mira-and-tgicl-ass_franssen_9376
SEQUENCE=CTTTACYTGAAGTTCATTCTAATCTCTTCCACTCTCAATTCCTTGCTCTCTCTCTTTTCATTCTTCAGGCGAATCCCTCAACCTTTCTGCATCCATCACACATCACGATCCAATAGTCATTCTTCGCTTTTCTTGATTCAGTAGCAGAAACATAAGGAAGAAGAGGAATTGAAGAAGAAGAATAGAAGAAGAAGAAGCGTGAGGAAGAACGATTAATACTTGTTCTTGCAAGACGCGCCGGATTCCGGTAGGTGATTTACCACTTTCTAATAACTTATTTGACGTGGTATTACTACTGAATCTCGTGATGCAATCGCTTTCGACTTAGAACCGCAAACTTCGCCATACATCGGATGTGAAATATGGATTTTTACGGCGTGAAATTACGAACCAGAAAGCACGCAAGATCATCACAAGATGCTATTTAATTTGAAAACTTTAGCGTGGGTTCAACTCTTCTCCTCTTCAATTCTGGATCGAGCTTTTGAAGTGGAGCGAGGAAGCGATTCATGTGGAACTGGTGATGGAAGCTCTTGAGCGGCGCAGACTCGGTCTCACGAGSAATTCCCCGA
TARGET=46,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00994 Ps_contig_mira-and-tgicl-ass_franssen_9376
SEQUENCE=CTTTACYTGAAGTTCATTCTAATCTCTTCCACTCTCAATTCCTTGCTCTCTCTCTTTTCATTCTTCAGGCGAATCCCTCAACCTTTCTGCATCCATCACACATCACGATCCAATAGTCATTCTTCGCTTTTCTTGATTCAGTAGCAGAAACATAAGGAAGAAGAGGAATTGAAGAAGAAGAATAGAAGAAGAAGAAGCGTGAGGAAGAACGATTAATACTTGTTCTTGCAAGACGCGCCGGATTCCGGTAGGTGATTTACCACTTTCTAATAACTTATTTGACGTGGTATTACTACTGAATCTCGTGATGCAATCGCTTTCGACTTAGAACCGCAAACTTCGCCATACATCGGATGTGAAATATGGATTTTTACGGCGTGAAATTACGAACCAGAAAGCACGCAAGATCATCACAAGATGCTATTTAATTTGAAAACTTTAGCGTGGGTTCAACTCTTCTCCTCTTCAATTCTGGATCGAGCTTTTGAAGTGGAGCGAGGAAGCGATTCATGTGGAACTGGTGATGGAAGCTCTTGAGCGGCGCAGACTCGGTCTCACGAGSAATTCCCCGA
TARGET=171,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00995 Ps_contig_mira-and-tgicl-ass_franssen_9376
SEQUENCE=CTTTACYTGAAGTTCATTCTAATCTCTTCCACTCTCAATTCCTTGCTCTCTCTCTTTTCATTCTTCAGGCGAATCCCTCAACCTTTCTGCATCCATCACACATCACGATCCAATAGTCATTCTTCGCTTTTCTTGATTCAGTAGCAGAAACATAAGGAAGAAGAGGAATTGAAGAAGAAGAATAGAAGAAGAAGAAGCGTGAGGAAGAACGATTAATACTTGTTCTTGCAAGACGCGCCGGATTCCGGTAGGTGATTTACCACTTTCTAATAACTTATTTGACGTGGTATTACTACTGAATCTCGTGATGCAATCGCTTTCGACTTAGAACCGCAAACTTCGCCATACATCGGATGTGAAATATGGATTTTTACGGCGTGAAATTACGAACCAGAAAGCACGCAAGATCATCACAAGATGCTATTTAATTTGAAAACTTTAGCGTGGGTTCAACTCTTCTCCTCTTCAATTCTGGATCGAGCTTTTGAAGTGGAGCGAGGAAGCGATTCATGTGGAACTGGTGATGGAAGCTCTTGAGCGGCGCAGACTCGGTCTCACGAGSAATTCCCCGA
TARGET=184,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00996 Ps_contig_mira-and-tgicl-ass_franssen_9377
SEQUENCE=AAACTCTCCCGGCTTTCCCAGGTTGACTCTTTCTTCCACAATACTCGTTACTCTTCATATGAATCGCTCTCTCTCCCTCTCTCGCTCYCTCTCTCTCTTACTTCCACTTCATCACTACTATACTATATTAAAGTTTTAAAAATGAAAACTTTCAATATGGGGTTTGATTGTTTTGTACTTTCCTACAAGGGTTTTCAACAAGCAACAATTTTGGACTTGCTTTTACTCTTTAGATATTGAAGAAAAAGAAAACTATTTTTTTGTTGAGAGAAAGAGAGAGAGAGTTGGTTTTTGG
TARGET=89,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00997 Ps_contig_mira-and-tgicl-ass_franssen_9377
SEQUENCE=AAACTCTCCCGGCTTTCCCAGGTTGACTCTTTCTTCCACAATACTCGTTACTCTTCATATGAATCGCTCTCTCTCCCTCTCTCGCTCYCTCTCTCTCTTACTTCCACTTCATCACTACTATACTATATTAAAGTTTTAAAAATGAAAACTTTCAATATGGGGTTTGATTGTTTTGTACTTTCCTACAAGGGTTTTCAACAAGCAACAATTTTGGACTTGCTTTTACTCTTTAGATATTGAAGAAAAAGAAAACTATTTTTTTGTTGAGAGAAAGAGAGAGAGAGTTGGTTTTTGG
TARGET=273,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00998 Ps_contig_mira-and-tgicl-ass_franssen_9378
SEQUENCE=GTCGGGGGTTGGTAAAAACCGTATTAGTCCATTTTAGATAACCTCTGTTATCGTCGTTCATTSACGCCTCACTCCACCAATGGCTTCTTCTCTCCACCAAAACCCCCACCTTCCTTAAACCCCACCACCACCCTCAATCCCCAAAACCCACCACCACCACACGCCGGAGCTACATCCCGGTACAGTGCGGAGGACCGCGTTCCCAGCGTGGACCACTAGTAAAAGGCCGTTTCCTAAGCATCGAAGCAATCCAACGCCATCYWAMCCMWAAACSMCGTCCACCGAACCAAACCCCAACCCGAACCCAAACCTACCTAACTCCTAACCCAAACCACGCTAACCCGTACCTTAATTA
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_00999 Ps_contig_mira-and-tgicl-ass_franssen_9393
SEQUENCE=GAATTGTTTCCACACAAGAGAGAATCAAGAGTCCCTTTGTCGATAACAGAACCAAAAGTCTTTGATTCAAAAGCACTCATGTCTCTTACATCCATTTTCAAATACTTCAACTGAGGACAATTTCGGTATTTATTCTGCATAGCATCAATCACCACAGAAGATATATCAATGTTGACAACATCTGTGTAACCACCTTCATCAACCATTCCTTCACTAAACGCTGAATTGCCGGAACCAACAACGAGGATGGATTGGTTTGGAGGAACATAGAGATTGATGATAGGAGCCAAAGTGATATACTTCTGGTACCAATCAAAAGGACCTGGTTCGTTTGTGTAACGGTTGTCCCAGTACCATGATTCACCGTAGGCTTGTGTACCTGTTGCCATCACTGTTGTTCAATGTTGATGGAAGTGTTTGGAGCAAGATGAATGATGAAGTTGTTGTTTTTGGTTGTTTCATTCAAATCTGAAAATTTTGGATCCCGAATCAGATCAATGAAATTGAAGTGCCACGTCTTACACACACACACTTGGCATCGGGCATTAGGCTATGTTSGTGACATTCCT
TARGET=521,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01000 Ps_contig_mira-and-tgicl-ass_franssen_9399
SEQUENCE=GAAGAATAACATTTTTCATAAAGGATATGTCTTAGCATAGATAAATTGATATAAGGATTCTATCCTTTCCTTTCAACGCCATCCCTCATGGCCCGCTTTCAACTACCCACCCTCCTCCTCCTCATCGCCACCGTCCCCCTCATCTCCGCTCTCCATGACCTCGCCGGAGATTTTCTCCGATTACCCTCCCAAGCTTCCACATTCCTCCACGACAACAACGAAGGCACCCGATGGGCCATCCTAATCGCTGGATCCAATGGCTACNGGAATTACCGCGCNGTCTGATGTATGTCACGCGTA
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4513
PRIMER_LEFT_PENALTY=0.263023
PRIMER_RIGHT_PENALTY=0.188292
PRIMER_LEFT_SEQUENCE=TTCTATCCTTTCCTTTCAACGC
PRIMER_RIGHT_SEQUENCE=GGAGGGTAATCGGAGAAAATCT
PRIMER_LEFT=57,22
PRIMER_RIGHT=188,22
PRIMER_LEFT_TM=59.737
PRIMER_RIGHT_TM=59.812
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.6275
PRIMER_LEFT_1_PENALTY=0.263023
PRIMER_RIGHT_1_PENALTY=0.364479
PRIMER_LEFT_1_SEQUENCE=TTCTATCCTTTCCTTTCAACGC
PRIMER_RIGHT_1_SEQUENCE=TACGCGTGACATACATCAGACN
PRIMER_LEFT_1=57,22
PRIMER_RIGHT_1=299,22
PRIMER_LEFT_1_TM=59.737
PRIMER_RIGHT_1_TM=60.364
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.0500
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=1.2702
PRIMER_LEFT_2_PENALTY=1.081865
PRIMER_RIGHT_2_PENALTY=0.188292
PRIMER_LEFT_2_SEQUENCE=CTATCCTTTCCTTTCAACGCC
PRIMER_RIGHT_2_SEQUENCE=GGAGGGTAATCGGAGAAAATCT
PRIMER_LEFT_2=59,21
PRIMER_RIGHT_2=188,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=59.812
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=130
PRIMER_PAIR_PENALTY_3=1.3805
PRIMER_LEFT_3_PENALTY=0.263023
PRIMER_RIGHT_3_PENALTY=1.117486
PRIMER_LEFT_3_SEQUENCE=TTCTATCCTTTCCTTTCAACGC
PRIMER_RIGHT_3_SEQUENCE=GTTGTTGTCGTGGAGGAATGT
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=218,21
PRIMER_LEFT_3_TM=59.737
PRIMER_RIGHT_3_TM=59.883
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=1.4463
PRIMER_LEFT_4_PENALTY=1.081865
PRIMER_RIGHT_4_PENALTY=0.364479
PRIMER_LEFT_4_SEQUENCE=CTATCCTTTCCTTTCAACGCC
PRIMER_RIGHT_4_SEQUENCE=TACGCGTGACATACATCAGACN
PRIMER_LEFT_4=59,21
PRIMER_RIGHT_4=299,22
PRIMER_LEFT_4_TM=60.082
PRIMER_RIGHT_4_TM=60.364
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=11.1000
PRIMER_RIGHT_4_END_STABILITY=7.0500
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_01001 Ps_contig_mira-and-tgicl-ass_franssen_9400
SEQUENCE=AGTATAAAKTTGATATAAGGATTCTATCCTTCCTTCTSAACGCCATCCCTCATGGCCCGCTTTCAACTACCCACCCTCCTCCTCCTCATCGCCACCGTCCCCCTCATCTCCGCTCTCCATGACCTCGCCGGAGATTTTCTCCGATTACCCTCCCAAGCTTCCACATTCCTCCACGACAACAACGAAGGCACCCGATGGGCCATCCTAATCGCTGGATCCAATGGCTACTGGAATTACCGACATCAGTCTGATGTATGTCACGCGTATCAAGTGTTGAGGAAAGGTGGTTTAAAAGAGGAGAATATTATTGTTTTCATGTATGATGATATTGCTTACGACGAAGAGAATCCACATCCTGGAGTCATCATTAACAGTCCAAATGGAGAGAATGTTTACAAGGGAGTCCCTAAGGATTATACCGGTGAAGATGTTACTGTCGGCAATTTCTTTGCTGCTTTGCTTGGAAATAAGTCAGCTCTTACCGGTGGGAGTGGGAAGGTTGTGGATAGCGGTCCCAATGATCATATATTTGTTTACTATAGTGATCATGGAGGTCCTGGAGTGCTTGGGATGCCCACTAGTCCGTACATGTACGCATCCGATCTAATTGAAGTCTTGAAGATAAAACATGCTGCTGGAACTTATAAAAGCCTAGTATTTTATCTAGAGGCATGCGAATCTGGGAGTATCTTTGAAGGTCTTCTTCCTGAAGGTCTGAATATCTATGCAACTACTGCCGCAAACGCAGAAGAAAGCAGTTGGGGAACATATTGTCCTGGGGAGAATCCTAGTCCTCCCCCAGAATATGGAACCTGCCTGGGTGACCTATATAGTGTTGCTTGGATGGAAGACAGTGACAAACACAATTTGCAAACAGAAACTTTGCACCAACAATATGAATTGGTCAAAGAAAGGACTTCAAACGGGAATTCAAATTATGGTTCCCATGTGATGCAATATGGTGACATTGAGCTTAGCAGGGATAGTCTATTCCTATATTTGGGATCAAATCCTTCAAATGAAAATTTTACTTTTGTGGGTAGAAACTCACTAGTGCCACCTTCCAAAGCGATCAACCAGCGTGATGCAGATCTAATCCATTTCTGGGATAAGTTCCGCAAAGCTCCTCAAGGTTCTCCTAGGAAAATTGCAGCTGAGAAGCAAGTTCTGGAAGCAATGTCTCACAGATTGCATATAGATGACAGCATAAAACTTATTGGAAAACTTCTATTTGGCATGAAAAAGGGTCCAGAGGTGCTCACCAGTGTTAGACCAGCTGGGCAGCCTCTTGTTGATGACTGGGACTGCCTCAAAACACTGGTTAGGACCTTTGAGACATATTGTGGATCTCTATCTCAGTATGGGATGAAACACATGCGGTCCTTTGCAAACTTCTGCAACGCAGGAATACACAGAGAGCATATGGCTGAGGCCTCTGCACAAGCATGCACCAATATTCCAGTCAATCCCTGGAGCTCTCTGCACAGTGGTTTCAGCGCATAATTCATCGCTCATTATTGAAGGTTAATGTATAATTGTCATGGGACTGCTTTATAATTGTTATATATAACATATATTGTACATGGTGTCATTATTTGTTCACACCTCTGTAAATACAATGAGGGACCGTGTGAATTGTGTGTATCTATGCGAATTAAAACAGAGGCAGTAACTTTTGCATTTTACTGAAAGATATTGCACCAAAAGGGGCCTAGCAACGTCTAATGTGAGGTCATGTTTATATATGAAATGCAGTTTTATTTATAGTACGTTATAGTAACCTGGTTGTGTTAAACTTTTAGTAAGAAAAATGTTATTTGACTATTTTAATTTTANAGAAACT
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01002 Ps_contig_mira-and-tgicl-ass_franssen_9408
SEQUENCE=CGGGGGATACGAACCCTAGTGTATTTTTGTCCTAGATCACAACTTTATAAGVAACCACAAATAAAAATAACACTCACACGATTTCATTGACTCTTCATCTCTCTCTCTCTCTCTCTCTSTGTRCCAATGGCGGATCCAATCTCCAAGCCTCTGATTGCGTTTCTGCTCATAGCTTCTCTGCTGCTGTGCTCACAAGCTTCCTCTGACGTTCCCTTCATCGTCGCTCACAAGAAGGCTTCTCTCAACAGGCTCAAGACTGGCG
TARGET=98,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01003 Ps_contig_mira-and-tgicl-ass_franssen_9412
SEQUENCE=AATATTAATTAATAAAAGTAAAGAAAAACAAAATTTTTAACTTAAAAATAAAAATAAAGATTTTAACTTAATATAGAATTAAGACAAAAATTAGGAAAATTTAAAAGAATATAATTATTAACTTGTAACATCCTGGAACAATATTCTGCAAGCATCTTAATTTCTCATTGATTTTCCCTCTTCTAACCCTTTCTGCTAAACTGTGACTATCAGTAGCTTGACCTCTTCTTGCTCTAACATGAACAACTTCCTTTGCTTTTTCTTCTTCTGTTTCATAGCTTTTCACTTTTTTTCCTCTTCCACCACTAAATTTGGTTTTGCTTCCACTTTCAGAAACTACAGGAGTTGAATTTGCAGAACTAGTCTCTTGAAAATCCATCATTTTCCTCTTTTTACCATCACGAACTTTATTTTCTTCTTGAAGATTTTGAAGTGAAACTTGAACTGCATTATTAACAAGATGATGATGATGATGATGAACAAGACCATGAAAATTCTCTTCCAAGTTTCTTGGAAACACGTGTTCTTGTTCTTGTTGATGTTGCTGATGATGTTCCAAG
TARGET=460,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2691
PRIMER_LEFT_PENALTY=1.269097
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=TGGTTTTGCTTCCACTTTCAG
PRIMER_RIGHT_SEQUENCE=CTTGGAACATCATCAGCAACAT
PRIMER_LEFT=312,21
PRIMER_RIGHT=559,22
PRIMER_LEFT_TM=60.269
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=1.5011
PRIMER_LEFT_1_PENALTY=1.501110
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=TTGGTTTTGCTTCCACTTTCAG
PRIMER_RIGHT_1_SEQUENCE=CTTGGAACATCATCAGCAACAT
PRIMER_LEFT_1=311,22
PRIMER_RIGHT_1=559,22
PRIMER_LEFT_1_TM=61.501
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=1.6298
PRIMER_LEFT_2_PENALTY=1.629805
PRIMER_RIGHT_2_PENALTY=0.000037
PRIMER_LEFT_2_SEQUENCE=ACAGGAGTTGAATTTGCAGAAC
PRIMER_RIGHT_2_SEQUENCE=CTTGGAACATCATCAGCAACAT
PRIMER_LEFT_2=338,22
PRIMER_RIGHT_2=559,22
PRIMER_LEFT_2_TM=58.370
PRIMER_RIGHT_2_TM=60.000
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=1.6548
PRIMER_LEFT_3_PENALTY=1.654741
PRIMER_RIGHT_3_PENALTY=0.000037
PRIMER_LEFT_3_SEQUENCE=GGTTTTGCTTCCACTTTCAGA
PRIMER_RIGHT_3_SEQUENCE=CTTGGAACATCATCAGCAACAT
PRIMER_LEFT_3=313,21
PRIMER_RIGHT_3=559,22
PRIMER_LEFT_3_TM=59.345
PRIMER_RIGHT_3_TM=60.000
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=1.7615
PRIMER_LEFT_4_PENALTY=1.761496
PRIMER_RIGHT_4_PENALTY=0.000037
PRIMER_LEFT_4_SEQUENCE=TCCTCTTTTTACCATCACGAAC
PRIMER_RIGHT_4_SEQUENCE=CTTGGAACATCATCAGCAACAT
PRIMER_LEFT_4=384,22
PRIMER_RIGHT_4=559,22
PRIMER_LEFT_4_TM=58.239
PRIMER_RIGHT_4_TM=60.000
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=176
=
PRIMER_SEQUENCE_ID=SSR_01004 Ps_contig_mira-and-tgicl-ass_franssen_9443
SEQUENCE=GGAAGGGTGGATAACAGAATGAAGAGATAAGAGAAAGATTGAAAGAGTGAAGAAAACTCGTGGCCCGAGCCAATGGTGAGAACTGCCAATTTATAGAGTTTCTATGAAGTGATTTTGTTAACTCTGTTAATCTCTTCTCCCACCAAATTTGTTAATCGTTGAAACTCTGTTAGTTAGTTAGTTTTTTAAATTGATTCTTCTGTTAAATTTCTGTTGACAATCTCAAATGGCTTTTGATTCTCTGTGGTGAATGCAGCTTCTGTTATCATGGATGGTTCGAAAAAATAGCAGGTGTTTTGAACTTGGATGCGAC
TARGET=169,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0760
PRIMER_LEFT_PENALTY=0.071684
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=AAGAGTGAAGAAAACTCGTGGC
PRIMER_RIGHT_SEQUENCE=ACCACAGAGAATCAAAAGCCAT
PRIMER_LEFT=42,22
PRIMER_RIGHT=247,22
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.1116
PRIMER_LEFT_1_PENALTY=0.071684
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=AAGAGTGAAGAAAACTCGTGGC
PRIMER_RIGHT_1_SEQUENCE=ATCCAAGTTCAAAACACCTGCT
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=307,22
PRIMER_LEFT_1_TM=59.928
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.1895
PRIMER_LEFT_2_PENALTY=0.071684
PRIMER_RIGHT_2_PENALTY=0.117817
PRIMER_LEFT_2_SEQUENCE=AAGAGTGAAGAAAACTCGTGGC
PRIMER_RIGHT_2_SEQUENCE=GCATTCACCACAGAGAATCAAA
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=253,22
PRIMER_LEFT_2_TM=59.928
PRIMER_RIGHT_2_TM=60.118
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.1895
PRIMER_LEFT_3_PENALTY=0.071684
PRIMER_RIGHT_3_PENALTY=0.117817
PRIMER_LEFT_3_SEQUENCE=AAGAGTGAAGAAAACTCGTGGC
PRIMER_RIGHT_3_SEQUENCE=GCCATTTGAGATTGTCAACAGA
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=230,22
PRIMER_LEFT_3_TM=59.928
PRIMER_RIGHT_3_TM=60.118
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.1913
PRIMER_LEFT_4_PENALTY=0.186949
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=GGAAGGGTGGATAACAGAATGA
PRIMER_RIGHT_4_SEQUENCE=ACCACAGAGAATCAAAAGCCAT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=247,22
PRIMER_LEFT_4_TM=60.187
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_01005 Ps_contig_mira-and-tgicl-ass_franssen_9444
SEQUENCE=GGAGAAACAACAAACGTAGTACTAGTAGTAGTAATGGTCCTACTTCTACTACTACTCTCATCAAAAGACCAATATCCACCATTGAAACCGCTACTACTTCTAATTCTTCTTCTCCTTCTTCAACTCATAGTTCAACTTCAAATCATATGAACCCTATGTTTTATGGCTTATCTAGTACTAATAACCCTTGTGATCCGAATCTTCCATTTTCAAGGTTCAATATTACTTCTAGGCTCTCAACAAGCTCAGGTTATGATCTTCAGCCTCAGATGAATTTTTTTTGGATTAGGTTTTTCATCTGGGTTTGAAAATAATGGTTATACAAACGGGTTTAATACTTCAAATAATAACTATGATTCAATCTTTAGTTCTTCAACTTCTGCATCAAATAATACATCAGTTATGCCTTCTGTGTTGAGCTCTACTCTACTGCAACATAAATTCTTCGACGATGGTTTGAAATATGGTTCAGATGCAGGTAGCAACGGCGCTTTCCAAGACTTACAATTTGGCTCAAAAATGCAAAACCAGATGGAACATATTGGTGGTTTTTATGATCCTGCTTCTTCAATTTACTTGAATGCAACGTCATCATCAGCTATTGGTGTTTGGAATGACCAAGGTGCAAATAATATTGGTTCATCCGTCACTTCTCTGATCTAGAATGAATAATCCACTCAAGTTCAAGTTCAAGTTCAAGGTCAAGGTTATCTTTAATTTGGTCCTTTTGTTTAGTTTTACTGATTAGGTTTCTGTGTTTTTTGTTTTGTTTTGTATTTTTGGGTTGGGTTTAGTTACATTTAACTTTATTGAAAAAAGAAAAAGAAGGAAAAAAAAAGATACTTGTTAGAAAAATGGGAAAGTTAAAGGTGTGAG
TARGET=760,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.7092
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=2.705594
PRIMER_LEFT_SEQUENCE=CAGCTATTGGTGTTTGGAATGA
PRIMER_RIGHT_SEQUENCE=CTCACACCTTTAACTTTCCCAT
PRIMER_LEFT=595,22
PRIMER_RIGHT=875,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=57.294
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=2.7092
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=2.705594
PRIMER_LEFT_1_SEQUENCE=TCAGCTATTGGTGTTTGGAATG
PRIMER_RIGHT_1_SEQUENCE=CTCACACCTTTAACTTTCCCAT
PRIMER_LEFT_1=594,22
PRIMER_RIGHT_1=875,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=57.294
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=2.7092
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=2.705594
PRIMER_LEFT_2_SEQUENCE=CATCAGCTATTGGTGTTTGGAA
PRIMER_RIGHT_2_SEQUENCE=CTCACACCTTTAACTTTCCCAT
PRIMER_LEFT_2=592,22
PRIMER_RIGHT_2=875,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=57.294
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=284
PRIMER_PAIR_PENALTY_3=3.0752
PRIMER_LEFT_3_PENALTY=0.369628
PRIMER_RIGHT_3_PENALTY=2.705594
PRIMER_LEFT_3_SEQUENCE=ATTGGTTCATCCGTCACTTCTC
PRIMER_RIGHT_3_SEQUENCE=CTCACACCTTTAACTTTCCCAT
PRIMER_LEFT_3=633,22
PRIMER_RIGHT_3=875,22
PRIMER_LEFT_3_TM=60.370
PRIMER_RIGHT_3_TM=57.294
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=3.2075
PRIMER_LEFT_4_PENALTY=0.501869
PRIMER_RIGHT_4_PENALTY=2.705594
PRIMER_LEFT_4_SEQUENCE=TCATCAGCTATTGGTGTTTGGA
PRIMER_RIGHT_4_SEQUENCE=CTCACACCTTTAACTTTCCCAT
PRIMER_LEFT_4=591,22
PRIMER_RIGHT_4=875,22
PRIMER_LEFT_4_TM=60.502
PRIMER_RIGHT_4_TM=57.294
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_01006 Ps_contig_mira-and-tgicl-ass_franssen_9445
SEQUENCE=GGTCCTTTTGTTTTAGTTTTACTGATTTAGGTTTCTGTTGTTTTTTGTTTTGTTTTGTATTTTTGGGTTGGGTTTAGTTACATTTAACTTTATTGAAAAAAGAAAAAGAAGGAAAAAAARRATACTTGTTAGAAAAATGGGAAAGTTAAAGGTGTGAGAGAAGAGTGTACTTTGGTAACTTTGATCAGAGAGATCCTATGTCAGCTACATCCTTCAAATTACTTCAGCTAGCTGGTTGGTATGAAAAAATTTGTGTTTGTGTTACGATAAACTTATTCACTTTTTCCAATTATTACAGGTCCCTGTGTCAGTGATCGGGGTGTCGTGTTTCATAGGTTTTATGTTGTTTTTCAGTTTGTATTATGTGTAAATATTGGATGACATGAACTAGT
TARGET=43,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01007 Ps_contig_mira-and-tgicl-ass_franssen_9448
SEQUENCE=TGAATCCCTGGAGGTTGCTCAAGATCTTCCAAAGAAGTGATAATCCATTATATCGATTGGAAGCCATTGATTTTGGTCGAAGAATGGCTGTTGAGAGAGAAATTGAGAAGACAGAAAGTGCAGCACAACCAAATGCAGTTTTTGATGATAGTTCCAACTTTCTTATATATGCAACCTTGCTTGGGATTAAAATTGTTAATTTACACACCAATACAGTTGCTCGAATACTTGGAAAGGTGGAGAATAATGATAGATTCTTAAGTATAGCTTTATATCATGGGGAACGAAGTGGCAAAAAAGTGAGAAAGATTCCTTCAGCAGCTGCAAATGCTAATGAGAGCAAGGAGCCTTTGACAGATCCCACTCTCCTCTGTTGTGCCTTTCAAAAAGCATAGGATTTATTTATTTAGTCGAAGAGAACCTGAGGAGCCTGAAGATGCAACTAAGGGAAGAGACGTGTTCAATGAAAAGCCTCCCGCTGATGAACTTTTGGCAGTTTCAGATATTGGAAAGGCACTAACAACATCACTTCCTGACAATGTGAT
TARGET=397,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1973
PRIMER_LEFT_PENALTY=0.013714
PRIMER_RIGHT_PENALTY=0.183585
PRIMER_LEFT_SEQUENCE=AATGCTAATGAGAGCAAGGAGC
PRIMER_RIGHT_SEQUENCE=ACACGTCTCTTCCCTTAGTTGC
PRIMER_LEFT=326,22
PRIMER_RIGHT=459,22
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=59.816
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.3127
PRIMER_LEFT_1_PENALTY=0.129103
PRIMER_RIGHT_1_PENALTY=0.183585
PRIMER_LEFT_1_SEQUENCE=TTGCTCGAATACTTGGAAAGGT
PRIMER_RIGHT_1_SEQUENCE=ACACGTCTCTTCCCTTAGTTGC
PRIMER_LEFT_1=216,22
PRIMER_RIGHT_1=459,22
PRIMER_LEFT_1_TM=60.129
PRIMER_RIGHT_1_TM=59.816
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.3127
PRIMER_LEFT_2_PENALTY=0.129103
PRIMER_RIGHT_2_PENALTY=0.183585
PRIMER_LEFT_2_SEQUENCE=GTTGCTCGAATACTTGGAAAGG
PRIMER_RIGHT_2_SEQUENCE=ACACGTCTCTTCCCTTAGTTGC
PRIMER_LEFT_2=215,22
PRIMER_RIGHT_2=459,22
PRIMER_LEFT_2_TM=60.129
PRIMER_RIGHT_2_TM=59.816
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.3548
PRIMER_LEFT_3_PENALTY=0.171245
PRIMER_RIGHT_3_PENALTY=0.183585
PRIMER_LEFT_3_SEQUENCE=AGCTTTATATCATGGGGAACGA
PRIMER_RIGHT_3_SEQUENCE=ACACGTCTCTTCCCTTAGTTGC
PRIMER_LEFT_3=265,22
PRIMER_RIGHT_3=459,22
PRIMER_LEFT_3_TM=59.829
PRIMER_RIGHT_3_TM=59.816
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=0.3564
PRIMER_LEFT_4_PENALTY=0.172839
PRIMER_RIGHT_4_PENALTY=0.183585
PRIMER_LEFT_4_SEQUENCE=CAGCTGCAAATGCTAATGAGAG
PRIMER_RIGHT_4_SEQUENCE=ACACGTCTCTTCCCTTAGTTGC
PRIMER_LEFT_4=318,22
PRIMER_RIGHT_4=459,22
PRIMER_LEFT_4_TM=60.173
PRIMER_RIGHT_4_TM=59.816
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=142
=
PRIMER_SEQUENCE_ID=SSR_01008 Ps_contig_mira-and-tgicl-ass_franssen_9454
SEQUENCE=CGGTTTTTATTGATAATCATAATTTCTTATAAATGATAAATGTTTGCTTACAATCAAAAGCTTGAAAATGCACTGGAACAAAAAAGGAACCAGGGCAATGAGTAAGCTAAACTATACTTTGCTAGATACAAAAGCAAGACAAATAAAACAGCATGGTACAAGTTAGAAAAACTATGATAAACCCGCCATACTAGTACTACCAACAACCCTAACAATATTTTATAACGGTCTATCTCAAGTCAACATAAGATCATTCCCTCCCAGATGAGGGCGGAGGCAAACGCGGACTTCGTGTTCTTCTGGTCCGACGCGATGTTTTGGCACCATGATCTCCAGCACTGATCCTGGCTCGTCGTAATATGCTTCCAGATTAGCATCTTCAGGAATTCTGGTTGAGAGAGAGATTTCGCGGACAAACTCTCCAGGAGGGCAGTGTTCTGAGGATGCATCTGTAAGCTTGAATGTTCTATCACGTCTCTTGATGAATGGCTTTCGAGATGTGCTCATACAAGAAACTTTGATGATACCGTGAGTCAGAGTGTTCCTCCATGAAACTTTCACACTTGCAAGGTCTACAAAGGGTAGACTGATGATAATCAAGTAACCCTGTTCGTCCTCGTAGATAGTTTTTGCGGCTGTAACTGGTCCGTATACATTCTTTATCACCCCGCTGAAGTCATTCAACCAGTGCGGCTCACTTGGGTGCATTTCTATATCTTGGATCCGATCAGATGGAGGATTAACAGCAAGGTAACATTCTTCATCGTTTAGGTTACCGTTTAGGCTTCCATTTAGGGTTCCATTACCGTTCCCGTTTACATGAGGGAAAAAGTCTTTCCTCTTTTTTCCGTTGGCAGAAGGTAGGTCCAACAAATCGCCGTTGGTGTGGTTAGACAGAGAAGTTGACAGATTCAGCCCAGACCCATTAAGCAGCTTCTTAGGAGGGTGTCCATTTGCAGCACTCTTGATTGAAGGAAGAGGTCTCTCAAGCTCAAAGTCCGTGTTGGGAAGATTTCTCCACGAACTAAAATCACTTGCTTCAGTTGGTACTGTGAAATTCAAATCACGGCCAGTGAGTTCAATCCACCTTTTCCGATCATCCTCGTCAAGATTCATCAGATTGGGTGAAGGAAAAACCTCAATGCCGTGTACACACTGAGGGTTTGAAAGTCCTCTATAATGCTTCCTTTGCATCCGGTGGGATCGAACAAATCCTTTGTCAACGCTAAAAGGAAACGGACGCTCCCCCTGGCGAGAATGACCGTTCATGTAACTCCTCAGCTGCATCTTCCCCAAGGCATTTTCAGGCCTCTCCTTGAAAACCCACATGTACATATTTTCCATATCATGTTGAACCATGAAAACATCAAGATTGAGATCCGATTTCTCAAAACCCGAAATACCATTTCCGTCCCTGATAATCTTGGCCTTCGATTTATCATTCAACGACGGCTTAAAGTAAAAACTAAAAGAAAAACCAAGCACCCCATATGCTATCAACTCGCTTAGCACATTTTCTTCCAACCTTAGGCAAAGTAAGAAAAGTCTCAGTCTCATACCCTTGAGTACCAAGACCAACATCCAGAATATCACAAGGATCAGAATTCCACGACTGAGGCGGCGGACTACGTTCAGCAGAAAGAGGCAAATTAATATCCGGCGGACGAGAAAGTATGATCTGCCGATTCATCTCCAAGTCCAGTTCCTCATGAGAAGACGCACTCGAATCCATAGACAATAACGTTGAAGGGTGATGATTCTGATGATTCTCCATTGACAAAGAAGCGAAACAGGAATCGCCAATGCTACCTCATCTTGTTGATGAGTTCTATTATTGCTGGTTGATAAAATATTGCCATCCCAAAAACAATTCAACAAAATAACAACCTTATAAACACCTTCATCATCATATCAATCCCCACTATATCTCCCTTCCCATNAAACCCCTANATTTATCAATTGAATTTGAGACAAAACAAAACCAAAACC
TARGET=395,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0373
PRIMER_LEFT_PENALTY=0.018437
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=TGCTTCCAGATTAGCATCTTCA
PRIMER_RIGHT_SEQUENCE=TATCTACGAGGACGAACAGGGT
PRIMER_LEFT=360,22
PRIMER_RIGHT=624,22
PRIMER_LEFT_TM=59.982
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.0725
PRIMER_LEFT_1_PENALTY=0.018437
PRIMER_RIGHT_1_PENALTY=0.054021
PRIMER_LEFT_1_SEQUENCE=TGCTTCCAGATTAGCATCTTCA
PRIMER_RIGHT_1_SEQUENCE=TACGAGGACGAACAGGGTTACT
PRIMER_LEFT_1=360,22
PRIMER_RIGHT_1=620,22
PRIMER_LEFT_1_TM=59.982
PRIMER_RIGHT_1_TM=60.054
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.0725
PRIMER_LEFT_2_PENALTY=0.018437
PRIMER_RIGHT_2_PENALTY=0.054021
PRIMER_LEFT_2_SEQUENCE=TGCTTCCAGATTAGCATCTTCA
PRIMER_RIGHT_2_SEQUENCE=CTACGAGGACGAACAGGGTTAC
PRIMER_LEFT_2=360,22
PRIMER_RIGHT_2=621,22
PRIMER_LEFT_2_TM=59.982
PRIMER_RIGHT_2_TM=60.054
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=262
PRIMER_PAIR_PENALTY_3=0.0799
PRIMER_LEFT_3_PENALTY=0.018437
PRIMER_RIGHT_3_PENALTY=0.061436
PRIMER_LEFT_3_SEQUENCE=TGCTTCCAGATTAGCATCTTCA
PRIMER_RIGHT_3_SEQUENCE=AGCCGCAAAAACTATCTACGAG
PRIMER_LEFT_3=360,22
PRIMER_RIGHT_3=636,22
PRIMER_LEFT_3_TM=59.982
PRIMER_RIGHT_3_TM=59.939
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.1624
PRIMER_LEFT_4_PENALTY=0.018437
PRIMER_RIGHT_4_PENALTY=0.143968
PRIMER_LEFT_4_SEQUENCE=TGCTTCCAGATTAGCATCTTCA
PRIMER_RIGHT_4_SEQUENCE=CTATCTACGAGGACGAACAGGG
PRIMER_LEFT_4=360,22
PRIMER_RIGHT_4=625,22
PRIMER_LEFT_4_TM=59.982
PRIMER_RIGHT_4_TM=60.144
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_01009 Ps_contig_mira-and-tgicl-ass_franssen_9456
SEQUENCE=GGATGTTAGCATTCGACAGCWCGTRKGAGTATCACCACTACCAATACCATTCGTCTTCGAACCTTAGGCGGAGATTTCATACCCATTTTCCCATTTAAACCTCTCTCTCTCTATCTCTCCCTCAACTTCTCCTCCCAATTCCCATTACCATTCCTAGAGGTTGAGATGGCAACAGCAGCATCAGCAGCTACTTTTTCTATTGGAACCGCCCAAACAGGGAGGTCACTTTCTCCATCAAACCCTTTTGGTTTCAAATCCAATTCCCAGGTTAATTTCTCTGGCCTCAAGGCCATGTCATCTTTCTCTGGCAACGAAACTTGTGCTGCTCTTCGTGCAACTTTTGCTCCCAAAGCTGTAAAAGAAAACCAAAACCGAAACCACATCTTGCAGCCACAGGCATCCTACAAAGTAGCGCTTCTCGGTGCTGCCGGAGGGATTGGTCAGCCACTGGCACTTCTCATCAAGATGTCGCCTTTGGTATCCGACCTGCATCTCTATGATATCGCGAACGTTAAGGGAGTTGCTGCTGATATTAGTCATTGCAACACTCCTTCAAAAGTTGCGGATTTCACAGGAGCTGCTGAGTTAGCCAATTGTTTGAAAGGTGTGGATGTTGTTGTTATACCTGCTGGTGTTCCTAGGAAACCCGGGATGACTCGTGATGATCTTTTCAACATCAATGCCGGTATAGTTAGAGACTTGGTCTCTGCCGTTGCAGATAATTGCCCTGGGGCTTTTATTCATATTATTAGTAATCCGGTGAATTCTACTGTGCCTATTGCTGCTGAAATTCTGAAACAAAAAGGTGTCTATGATCCTAAAAAGCTCTTTGGTGTTAGCACACTTGATGTTGTGAGGGCAAACACCTTTGTTGCTCAGAGAAAGAACTTGAGGCTGATTGATGTAGATGTCCCTGTTGTTGGGGGGCATGCTGGGATTACCATTCTTCCTCTTTTGTCAAAGACAAAACCCTCGGCGAGTTTCACTGATGAAGAAATTGAGGAGCTGACTGTCAGGATTCAAAATGCTGGAACTGAAGTTGTTGAGGCAAAAGCTGGTGCAGGGTCTGCAACTTTGTCAATGGCTTATGCAGCAGCTAGATTTGTTGAATCCTCTCTTCGTGCGCTTGACGGAGATGCTGATGTGTACGAGTGCTCATATGTACAGTCAGATCTGACTGACCTTCCGTTTTTTGCTTCAAGGGTGAAGATTGGTAGGAAAGGAGTCGAGGCTTTCATTCCAACTGATCTCCAAGGATTGAGTGAGTACGAGCAGAAGGCTTTGGAAGCACTCAAGCCAGAACTTAAGGCCAGTATCGAGAAGGGGGTTGCTTTTGCTCAAAAGCAAACTGTTGTCGCTTAACTTATTTTGCAAATGCGTTTATGCAATAATCTCGTGTCCTTTTGAGAGGATGAATACTACTTAGAGAACTTAGGTGGCAGATTTATTGTAGAATCATTTACTGCTTCCAGAATTTAGGACTTGAATTTTCTGTTGTATCAATTCTGCTTCCAGACTTGTTAGTAGAACGGGTGGGGGCTTCCATTTCTGTTCTCTATTTTGAGCTTTAGTTTTGATCAGAAATATCAATAGATTGTTACTATAATGTACTCTACTAGAATAAGAAAAATGTAAACTTTGCATTTTGAATAATATGGCCATTGGAGACGTTTGTAGTTTYT
TARGET=101,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01010 Ps_contig_mira-and-tgicl-ass_franssen_9461
SEQUENCE=TTAAGAGCTATAAAACTACCAGTTAAACCTTCAACACAAAAGACTCCTGCAAATCTGAAGGCAAATGACACCAAGCAAGACTTGAATCCCTTTAGCTCTCTTGGGGAGCTCAAACAATGAAACATTGAAGTGACCCTCTGATGATTCCASTATATTCGCATACCCAATTCTCAAGAAATGAATTAGGTGAACTTCGTCCAAGTCAAGCACAAACTTGTATGGCTCTACAGAAGTCCTCAGTTCACTCATCTTCATGAGCAGCAGTATGTGTGTGTGCGCGCTCCAAAAGCTCCTCACAAAATACCAAATGTCCACAAGAGAAACATAGTCATGTAACTCATAGAAGATGCAAGAAGCATCCTTCGTAAACCGAGATTAGAGGAAAGGAT
TARGET=267,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01011 Ps_contig_mira-and-tgicl-ass_franssen_9472
SEQUENCE=CAACTCCTTCTAGTGTTGTTGCTAATCCAAACCCTCCTTCTCAATCTCAACAACAACAACAACAACAACAACAGCACCATAGTTTTGATATTGCTGCTACCTCAAACCA
TARGET=48,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01012 Ps_contig_mira-and-tgicl-ass_franssen_9473
SEQUENCE=AAAGGGCGTGTTTCGAAGTAGGGGGAGGGTTAGGCGTGAGGGTTACGAAACTAGCTGCTAGGGAGATAACTTCATGAGGAGGAGGAAGATGTGGTGGAGGAGGAGAGGATGAAGGAGAGGGAGATGAGGGATAAGTCGGATATGAAAGAGAGGGAATTCATTGTTCACGTGCCGTTGCCTGATGAAAAGGAGATTGAGAAGATGGTGTTGCAGAAGAAGAAGACGGATTTGTTGAATAAGTATGTTAGTGATGTTCTCATGGAGGAGCAGACTGAAGCTAAAGACTTGCTTAACATTCATCGCTAGCTAGGTAGGTGATTTTTATGTTCTGCCCCGAATTGGATTGGAACTGTTTACTTACATCAATACATGTTGCAGTTGATTTAGACTAGTATGTTTGATGTTTTCTGAATGACTTATCTATTGTTGTAAATTGCACTGGATGATGTTGTATTCATTAAGAGAATTTAAATGCATATAGTGTAGCATTGTATTTGC
TARGET=212,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5320
PRIMER_LEFT_PENALTY=0.073866
PRIMER_RIGHT_PENALTY=0.458124
PRIMER_LEFT_SEQUENCE=TTCATGAGGAGGAGGAAGATGT
PRIMER_RIGHT_SEQUENCE=GCTAGCGATGAATGTTAAGCAA
PRIMER_LEFT=70,22
PRIMER_RIGHT=306,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.542
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.5529
PRIMER_LEFT_1_PENALTY=0.401769
PRIMER_RIGHT_1_PENALTY=0.151106
PRIMER_LEFT_1_SEQUENCE=GTTGCCTGATGAAAAGGAGATT
PRIMER_RIGHT_1_SEQUENCE=TTTAGCTTCAGTCTGCTCCTCC
PRIMER_LEFT_1=173,22
PRIMER_RIGHT_1=281,22
PRIMER_LEFT_1_TM=59.598
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.5968
PRIMER_LEFT_2_PENALTY=0.445740
PRIMER_RIGHT_2_PENALTY=0.151106
PRIMER_LEFT_2_SEQUENCE=GTCGGATATGAAAGAGAGGGAA
PRIMER_RIGHT_2_SEQUENCE=TTTAGCTTCAGTCTGCTCCTCC
PRIMER_LEFT_2=134,22
PRIMER_RIGHT_2=281,22
PRIMER_LEFT_2_TM=59.554
PRIMER_RIGHT_2_TM=60.151
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=0.6622
PRIMER_LEFT_3_PENALTY=0.204119
PRIMER_RIGHT_3_PENALTY=0.458124
PRIMER_LEFT_3_SEQUENCE=CTTCATGAGGAGGAGGAAGATG
PRIMER_RIGHT_3_SEQUENCE=GCTAGCGATGAATGTTAAGCAA
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=306,22
PRIMER_LEFT_3_TM=60.204
PRIMER_RIGHT_3_TM=59.542
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.6659
PRIMER_LEFT_4_PENALTY=0.207756
PRIMER_RIGHT_4_PENALTY=0.458124
PRIMER_LEFT_4_SEQUENCE=GCCTGATGAAAAGGAGATTGAG
PRIMER_RIGHT_4_SEQUENCE=GCTAGCGATGAATGTTAAGCAA
PRIMER_LEFT_4=176,22
PRIMER_RIGHT_4=306,22
PRIMER_LEFT_4_TM=60.208
PRIMER_RIGHT_4_TM=59.542
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=131
=
PRIMER_SEQUENCE_ID=SSR_01013 Ps_contig_mira-and-tgicl-ass_franssen_9479
SEQUENCE=AAATTTGTTGGAATGAAGAAGATGAAGCCAAACGATAATTCCAGAGAAACTTCGAAGAGGGCTTGGCATGGAATGGTACAAGTTGGCAAGGCATGGTGATTAAGAGGTTTTGGAATGGCATGAAAGACTTGATGCCACAAATGGAGAATCCAATGGTATGAAGACTAAAGTAGGCTAGGGGATGATTAGCTTGACTTTGGTCAACTGTTTTACCAAAAATTCAATAATTGATCAAAAGTCAACCTAGGTCAAAATTAGGGTTTTCTTTGTGTGTGTGCTTTATAAATTTGGATTTTGTAATGTATTTTTGGTGTGTTGACCATGAATTATCCTTGTATGGTTGTAATTTGTTTTCCAAGCAATGCAAATGTTTTTACCCCC
TARGET=268,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3631
PRIMER_LEFT_PENALTY=0.008930
PRIMER_RIGHT_PENALTY=1.354147
PRIMER_LEFT_SEQUENCE=TTCCAGAGAAACTTCGAAGAGG
PRIMER_RIGHT_SEQUENCE=GGATAATTCATGGTCAACACACC
PRIMER_LEFT=38,22
PRIMER_RIGHT=331,23
PRIMER_LEFT_TM=59.991
PRIMER_RIGHT_TM=60.354
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=1.3762
PRIMER_LEFT_1_PENALTY=0.022096
PRIMER_RIGHT_1_PENALTY=1.354147
PRIMER_LEFT_1_SEQUENCE=AGAGAAACTTCGAAGAGGGCTT
PRIMER_RIGHT_1_SEQUENCE=GGATAATTCATGGTCAACACACC
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=331,23
PRIMER_LEFT_1_TM=60.022
PRIMER_RIGHT_1_TM=60.354
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=1.3882
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=1.354147
PRIMER_LEFT_2_SEQUENCE=GGGATGATTAGCTTGACTTTGG
PRIMER_RIGHT_2_SEQUENCE=GGATAATTCATGGTCAACACACC
PRIMER_LEFT_2=178,22
PRIMER_RIGHT_2=331,23
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=60.354
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=1.3882
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=1.354147
PRIMER_LEFT_3_SEQUENCE=GGGGATGATTAGCTTGACTTTG
PRIMER_RIGHT_3_SEQUENCE=GGATAATTCATGGTCAACACACC
PRIMER_LEFT_3=177,22
PRIMER_RIGHT_3=331,23
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=60.354
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=1.6816
PRIMER_LEFT_4_PENALTY=0.327468
PRIMER_RIGHT_4_PENALTY=1.354147
PRIMER_LEFT_4_SEQUENCE=GGAATGGCATGAAAGACTTGAT
PRIMER_RIGHT_4_SEQUENCE=GGATAATTCATGGTCAACACACC
PRIMER_LEFT_4=111,22
PRIMER_RIGHT_4=331,23
PRIMER_LEFT_4_TM=60.327
PRIMER_RIGHT_4_TM=60.354
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_01014 Ps_contig_mira-and-tgicl-ass_franssen_9483
SEQUENCE=CCCAACTCACGGATCTCTCAGCCTCTCTGGCTCATATCCTTGGATCAGGTCAGCAGCTTCCGCAGCTATACGCTGCCTTAAATTCTCACGAACTAAAGCACAGTCCTTCCCAAGCAAATACTCAAGTGCCTGTTTCGATCACATGCATCAAGCCAGATCCTGCTGTTGGGCTCCCAAAGCATTATGAACCAATGATTGGTAGTAATGAGCAAAAGAATGCTGATGCAAGAGGCATACCCCCAACCATTCCTCCAAGTAAAAATATTGCCAAAGTAGAAATGCTGTCACCGTTGTCTAATTTAGGAAAACAAAATTATGGGGATTCTATTAAGGGTGCCTCCTCAGAACTTATCAAGAGTGATAATCTAATCCGTTTGCAGCCTGGTCATAACACAGTGCTTTCTAACAATGAAGAGGTAGCCAAGGAAAGGAAAAATTCTCAAGACGGTCCTAAAAGTAACAAAAGAGAATGGTCCACAAAACATGGACCAAGATGGTAAACTTGATGATGACAAACAAACAAAGGATATGAAAGGGATTCGTGCATTTTAAATTTTGCACTGGCTGTAGTTTTGTTAAGGAGNTTTTGAAACCAGCCTGGGAAAAGAAGGTACAAAGTAAAATAAAGATGCTTAATAAAACAATTGTAGAAAG
TARGET=512,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2386
PRIMER_LEFT_PENALTY=0.088662
PRIMER_RIGHT_PENALTY=0.149895
PRIMER_LEFT_SEQUENCE=TATTAAGGGTGCCTCCTCAGAA
PRIMER_RIGHT_SEQUENCE=ACAAAACTACAGCCAGTGCAAA
PRIMER_LEFT=325,22
PRIMER_RIGHT=575,22
PRIMER_LEFT_TM=60.089
PRIMER_RIGHT_TM=59.850
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.2386
PRIMER_LEFT_1_PENALTY=0.088662
PRIMER_RIGHT_1_PENALTY=0.149895
PRIMER_LEFT_1_SEQUENCE=TATTAAGGGTGCCTCCTCAGAA
PRIMER_RIGHT_1_SEQUENCE=AACAAAACTACAGCCAGTGCAA
PRIMER_LEFT_1=325,22
PRIMER_RIGHT_1=576,22
PRIMER_LEFT_1_TM=60.089
PRIMER_RIGHT_1_TM=59.850
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.2649
PRIMER_LEFT_2_PENALTY=0.115007
PRIMER_RIGHT_2_PENALTY=0.149895
PRIMER_LEFT_2_SEQUENCE=TAATCTAATCCGTTTGCAGCCT
PRIMER_RIGHT_2_SEQUENCE=AACAAAACTACAGCCAGTGCAA
PRIMER_LEFT_2=361,22
PRIMER_RIGHT_2=576,22
PRIMER_LEFT_2_TM=60.115
PRIMER_RIGHT_2_TM=59.850
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=216
PRIMER_PAIR_PENALTY_3=0.2665
PRIMER_LEFT_3_PENALTY=0.088662
PRIMER_RIGHT_3_PENALTY=0.177853
PRIMER_LEFT_3_SEQUENCE=TATTAAGGGTGCCTCCTCAGAA
PRIMER_RIGHT_3_SEQUENCE=ATGCACGAATCCCTTTCATATC
PRIMER_LEFT_3=325,22
PRIMER_RIGHT_3=546,22
PRIMER_LEFT_3_TM=60.089
PRIMER_RIGHT_3_TM=60.178
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.2803
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.149895
PRIMER_LEFT_4_SEQUENCE=ACAATGAAGAGGTAGCCAAGGA
PRIMER_RIGHT_4_SEQUENCE=ACAAAACTACAGCCAGTGCAAA
PRIMER_LEFT_4=405,22
PRIMER_RIGHT_4=575,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.850
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_01015 Ps_contig_mira-and-tgicl-ass_franssen_9484
SEQUENCE=CTAACAATGAAGAGGTAGCCAAGGGAAGGAAAAATTCTCAAGACGGTCCTAAAAGTACAAAAGAGAATGGTCCACAAAACATGGACCAAGATGGTAAACTTGATGATGACAAACAAACAAAGGATATGAAAGGGATTCGTGCATTTAAATTTGCACTGGCTGAGTTTGTTAAGGAGCTTTTAAAACCAGCCTGGAAAGAAGGTCAAGTAAATAAAGATGCTTATAAAACAATTGTGAAGAAAGTTGTTGATAAAGTATCGAGTACCATGCAGGGGGCCAACATTCCTCAGACACAAGAGAAATTGACCAATATT
TARGET=109,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4243
PRIMER_LEFT_PENALTY=0.275749
PRIMER_RIGHT_PENALTY=0.148509
PRIMER_LEFT_SEQUENCE=CAAAACATGGACCAAGATGGTA
PRIMER_RIGHT_SEQUENCE=TCTTGTGTCTGAGGAATGTTGG
PRIMER_LEFT=74,22
PRIMER_RIGHT=297,22
PRIMER_LEFT_TM=59.724
PRIMER_RIGHT_TM=60.149
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.4956
PRIMER_LEFT_1_PENALTY=0.347129
PRIMER_RIGHT_1_PENALTY=0.148509
PRIMER_LEFT_1_SEQUENCE=AACAATGAAGAGGTAGCCAAGG
PRIMER_RIGHT_1_SEQUENCE=TCTTGTGTCTGAGGAATGTTGG
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=297,22
PRIMER_LEFT_1_TM=59.653
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=0.5134
PRIMER_LEFT_2_PENALTY=0.275749
PRIMER_RIGHT_2_PENALTY=0.237699
PRIMER_LEFT_2_SEQUENCE=CAAAACATGGACCAAGATGGTA
PRIMER_RIGHT_2_SEQUENCE=TTACTTGACCTTCTTTCCAGGC
PRIMER_LEFT_2=74,22
PRIMER_RIGHT_2=209,22
PRIMER_LEFT_2_TM=59.724
PRIMER_RIGHT_2_TM=59.762
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.5250
PRIMER_LEFT_3_PENALTY=0.347129
PRIMER_RIGHT_3_PENALTY=0.177853
PRIMER_LEFT_3_SEQUENCE=AACAATGAAGAGGTAGCCAAGG
PRIMER_RIGHT_3_SEQUENCE=ATGCACGAATCCCTTTCATATC
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=143,22
PRIMER_LEFT_3_TM=59.653
PRIMER_RIGHT_3_TM=60.178
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=5.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=142
PRIMER_PAIR_PENALTY_4=0.5848
PRIMER_LEFT_4_PENALTY=0.347129
PRIMER_RIGHT_4_PENALTY=0.237699
PRIMER_LEFT_4_SEQUENCE=AACAATGAAGAGGTAGCCAAGG
PRIMER_RIGHT_4_SEQUENCE=TTACTTGACCTTCTTTCCAGGC
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=209,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=59.762
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_01016 Ps_contig_mira-and-tgicl-ass_franssen_9488
SEQUENCE=AAAGTAGGNGGAAATTCATTTAGTCTTTTACTTCATTCATATRAATAAAAGAAMCCAAAAAAGGTAAAACAAMCATAAAATTACTAAAAACRMCCTAAAAATTACGATGTCATTACATTGTCTCAATTGAAATAGCAAAATCTTGTGAGTAAAACTAAAACTATAGCTAGAACCACAAATGTCTAACAAGTAACTCACTTCAGACCTTAGTCAAATAAGCTGAATCCCATGTCATCATCGGATTCTTCCTTCTCTTCCACCTTCTTCTCCTCCTTAGCTTCAGCAGCAGGGGCAGCAGCTGCAGCACCTCCTCCAGAAGCAGCAGAAAGAGCAACGGCACCACCACCACCACCAGATGGAACTGAAGCCAACTTTTCTCTTCCACTGGCAATAAGCTCAGCAAAATCTTTGCCTTTCACTTCACTTAGGAGTAACTCAATATTTGAGTCCTCAGCCTCAACTCCAACTGATCCGAGAATCTTCTTAATATCAGCGGCTGAAGGGGATGTGTTGCCTCCCAATACGGCCAACAAATAAGCGGCGATAACCTTCATTTTTGATGTAGAGGGAAGAGGAATGAGAACTCGATTTAGGGTTTCACCAGAGAGATATTGAGGCACACTGCCCCGACWCYCYGCGTTGATACCTA
TARGET=338,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01017 Ps_contig_mira-and-tgicl-ass_franssen_9511
SEQUENCE=GAAAAGCTGCACAGCTTTTTCTCTCTCCCTTTCCTTRAAAATGAGCCAAGTGGTCAGAGAGAGGAGAGAGGCACGGTAGAGGTTGTGGGAAAGAAGAAGAAGAAGTTTTGTTTTCTAGTTTTCTTTTTCGGTTTGCAGAGTGTGTAGTGAGTCTTRAATAGTGAGAGAAGAGGGGTCTTAATGATGAGTGGAGAGTGAGGTATAGTSMSTGAGAGAGATGGAGTGAGGAATTGGCAAATAGTGTGAGACC
TARGET=91,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01018 Ps_contig_mira-and-tgicl-ass_franssen_9513
SEQUENCE=AGAAACCTATAATGGACAGACTTGGAGAAGCTTATGACAGCCCAGACAAGAATTGGCATTGCAATTATGTTGGGCCATTGGAGAGCATGGTGGTGGTGGTGGATCTCACAAAGACGAAGCCCGTGAGCTTTTTGAGAGTTTAGAACTACTTCTGTATGAAAACCTTTCCTCAAGTCGTCTGGGCATGGCACATGAGTTATCCCTTAGTTCTGATAAGGATACCTACAGAAGGTCATCGCAGTCCAGGCTTATGTGTTTTGTTGTGACAGCCATTGCAAAACTTGCTACGTATCACCGCGTAA
TARGET=88,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1190
PRIMER_LEFT_PENALTY=0.086698
PRIMER_RIGHT_PENALTY=0.032347
PRIMER_LEFT_SEQUENCE=AGAAGCTTATGACAGCCCAGAC
PRIMER_RIGHT_SEQUENCE=CAACAAAACACATAAGCCTGGA
PRIMER_LEFT=25,22
PRIMER_RIGHT=262,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=60.032
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.2307
PRIMER_LEFT_1_PENALTY=0.086698
PRIMER_RIGHT_1_PENALTY=0.143968
PRIMER_LEFT_1_SEQUENCE=AGAAGCTTATGACAGCCCAGAC
PRIMER_RIGHT_1_SEQUENCE=ACGACTTGAGGAAAGGTTTTCA
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=177,22
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=153
PRIMER_PAIR_PENALTY_2=0.2699
PRIMER_LEFT_2_PENALTY=0.086698
PRIMER_RIGHT_2_PENALTY=0.183236
PRIMER_LEFT_2_SEQUENCE=AGAAGCTTATGACAGCCCAGAC
PRIMER_RIGHT_2_SEQUENCE=TACGTAGCAAGTTTTGCAATGG
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=290,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=60.183
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.3803
PRIMER_LEFT_3_PENALTY=0.086698
PRIMER_RIGHT_3_PENALTY=0.293633
PRIMER_LEFT_3_SEQUENCE=AGAAGCTTATGACAGCCCAGAC
PRIMER_RIGHT_3_SEQUENCE=CGGTGATACGTAGCAAGTTTTG
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=59.706
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=272
PRIMER_PAIR_PENALTY_4=0.4250
PRIMER_LEFT_4_PENALTY=0.392624
PRIMER_RIGHT_4_PENALTY=0.032347
PRIMER_LEFT_4_SEQUENCE=CTTGGAGAAGCTTATGACAGCC
PRIMER_RIGHT_4_SEQUENCE=CAACAAAACACATAAGCCTGGA
PRIMER_LEFT_4=20,22
PRIMER_RIGHT_4=262,22
PRIMER_LEFT_4_TM=60.393
PRIMER_RIGHT_4_TM=60.032
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_01019 Ps_contig_mira-and-tgicl-ass_franssen_9525
SEQUENCE=TCAAATGGAAACACCTCCTTGTCAAGAATTTTTTGTTAGTCTTTTATCATGTTGCAACGAACAGCAATCTTGCGAAAATCTTCTAAACTTAACCTTCCATCTCCGTCACTATCAAAGCAGCGAATCATATCAACCAACTCATTATCGGTCCATGCAAAATCATGAGTTATAGCTGCTCTTTCTAAATCCCTTATTGTTACAGTTCCTTTTCCAGCTTCATCAAGCTGGAAGAAGTGTACGATGAGTTCATCCTCAGTCATTTGCAGCCGACTAATAAACGATTTTTTGTTCTTCTTTTGTTCCTTATCTTCTTGAGTGTGAATGTTTTCCTTTTTCTCGGCCTTGCTGATGTTCCCTACATTCATGTTAGAGCAAACTGAAGGTTCTGCAGAATTCTGCAGAGAAAGAGCAATTGCCTGCATCAAAGCTTCATCTTCATCGTCAACATTATCTAAATTACCACCATGCTTCTTTCCGGCACTCTGCTTTTCACCTTCAAACTTTTGTTCTTCGCCTTCCTTTTCCGTGACCCAGAAGCATTTTCAAAATCAGACTCATGTTCACTGGAAGAATCTGATTCCGTTTCTCGTTCCTCTTCATTTTCGGGTCTATACTCTTCATCATCACCTTCTAACCCTTTTTCCTTTTTCCTTTTCCCCTTTTTCTTTGTTAATCTGAGGCAAGGTTTTCAAGGAAGAGGCCATTTGAGGTAACCCCAAAGCTTTCAACCTCGCTTGGTTTTCTGCAATTCTCCGTAGTCTTTGTTCCTCATACTCCGATATGCTACTCCTCCCATTACGTACGGTTTCTCCTGCTTCCTCCGATTGCGACGATGTACCAACCGATTGTGAATTGGATTTCGATTTCTTGCGTTTCGCCATTTTCTTATGMTCTTACAATTTCAATTCTGGTTCTACTTTTATTGGGGTTACGTTGTGGAAAAGAAAAAGTTGAGATAAATAAATAAATTCTTAAAAAAAATCCCGAC
TARGET=956,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01020 Ps_contig_mira-and-tgicl-ass_franssen_9534
SEQUENCE=TTCGACCGGGTTCAGCTACCCTCTCTCTCTAAGAAAGAACAAGAGTTTTGATAGACTCGTTTGTTTCGCCGTTGATGACGAAATCAGAGAGAAGCAACAACAACAGTTGAGTACTAGTTCCACTAGACTTGGCTCCGCCGTTGAAGAAAGACCCGATATGATTGATGGTATAAGTGAAGAAGCATTGGAAAATTTTAAGGAAGATGGGGAAAGAAGTGCTATCTACGATTTTCTTTATCCTAGTAAAGAGCTTCTACCCGATGATAAAGAGATGAGCATATTTGATCACCTTGAAGAGCTACGAGAGAGAATCTTTATATCAGTTCTGG
TARGET=21,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01021 Ps_contig_mira-and-tgicl-ass_franssen_9549
SEQUENCE=GAGTCACACATTAATTCCTCAACGTCTCTCTCTGACCAAAAGATAAAGGAAAGGATTAAAAACAARAAAGGGAGAGGTTCATCTCCACTACATGTTTCCTCTCTCATAAAAGATTCTCTAACAAGACTCTTAGAAGAAAGGAGAAGAAATAAAAAAGAGAGGAACAAGATTGTTCCTCTTTGTCCTCAACCACACGCTCTCTTCTTCTTCTTCCAACACATAACCTCTCATCTTCACAAAACTCCAAAACAAAACCTACTTCGCCGCAACCCACACAGAACTTTTCCGACCATCACCGCGAACACGCCAACAACCTCCTTCCGCCGCTATAGCCAAAACCCCCCCGCCTGCATCTTCTCCCTAAATCGTCGCCTCCGCCTCCACCACCTACGACCACCGTCACGCACACATCCCAC
TARGET=200,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01022 Ps_contig_mira-and-tgicl-ass_franssen_9550
SEQUENCE=CTCTCTAACACTTGAACCAATATTTTTCTTGCCTACTACAAAAACATTTAGAGTTTGAAGGTTTTCTAGTTCAACAATTTGCTTCGGCATCTTTATTATGCTTGTCCTATCAATATAAAGATGACGCAAATTAATTAATTTACCAACMTGTTCTGGCAATTYTGTGAGTTWTTGTAGCACTCAGATAAAATCAAGGTTTGCAAGTAGTAGWGGTTNCATNTGGTNTCAGGCAAGCTG
TARGET=129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01023 Ps_contig_mira-and-tgicl-ass_franssen_9562
SEQUENCE=TAGGCTGGCCGTAAAATTTTAGCAAAACTGACAGGCTTTGACTACAAGCTTAAACGAATATTTAACATGAATCACAAGACGGCAAACTAGCAGATAGTCTAACTTTCGATACCATTCTCGTGACCCCGCTCCATCTGTTCTTTAAACCCGAATTTGAACGATTTGGGAGTGATCGACATTTTGTTCAAGTCGGGGTCAAAATCAAGACCTGCTCCAACACTACCTATCATATCATCATCATCAATCATATCCTTTGCATTTAAGTCAACAAAATCGTCGTCTGATGCTTCACTTCCAGAATCACTCTCTTCCTCATCGCCATATGTAGTTCTGACTCTTGACCAGCGGACAAAGTGTGGTCTTAGGTAGCTTTCACTTTTCTTCAACAAACATGGATCAAATGGGAAGAACATGTCGAGTCTATCCATCCCACCAAAAGCCTTGGAAAGATCAGCCTCTAGCAAATCCTCAAAAACAAATAACTCGGCTGACATGAAAAGCCGAGCAGCCTTTGCCTGTTTAAGAAATTCTTCTACTACACTAGGCAAACACACCTTCAAGGGATTCAATTTATGTTTCCAGAGTAACAACATTGGCATTTTGATAAGCTGCATTCTAAGCCTAGGCACGTCCACCAATGATTTCATGCGAAAGCATAAAATGTACATGATAGCCTGACACCCAGAATAAAACACTTGGTGAGCTTGCGGGTTCATATCTAAATCATGTATCTTGCAGTATGAAAAACACCAATCTACCAATCTTTGTATGATGTCAGCAACCAACGCAGATGAAAGAAACTTTGCACGAGATAAATAGCTAGCAAGATAAGCAACAGCACTCATCCTAGTAATAGGGGGATTAACAGGTGATTCAAACATATCTCTGAGAACAATAGCCAATTTCACACCACATAGTTCAGGATCCAATGCACATGCATAGAACATCACAAACTGGGTAAATTTGGACTTGTATGTATTCAGCACAGTTCTCTTAAATGACATCAATAATATATCAAATACCTCAGACAATCGGCCACTACTTTGACAGGATTCAAGATGCAAAAAAGTCAGCACAATTAGGCTATCTAATTTCTCCACAACCTTATTGCCTTGCAATTTTTTCCTATTTAACAGCTCTGAGGCACACTGGACTCAACATRATTGHAACTTTTACCCCTTCACATAGCCGCTCAACCTGAACC
TARGET=231,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01024 Ps_contig_mira-and-tgicl-ass_franssen_9566
SEQUENCE=GGCSATAACAAAATCTTCCACCAAAAAACACTCATTCTCAGGCCGCCGCCGCCGCCTGCCGTTCGGACTGCAGTGAGCCGCCGAKSWCAGAACCACGAAAAACAGCATTGCCTGTATAAAATAATTTGAACTTCAGAGATGGAGTATGTCAGCCCGGAGGGACTTCGCTTGGATGGTCGCCGACCCATGGAAATGCGACAAATTCGAGCAGAGATTGGCGCTGTATCCAAAGCTGACGGCTCTGCCGTTTTCGAGATGGGTAATACCAAAGTGATTGCTGCTGTTTATGGCCCTAGAGAGGTTCAAAATAGGAGCCAACAACTGAGTGACAAGGCTCTGGTTAGATGTGAATACAACATGGCTAATTTTAGCACGGGTGATCGCATGAGGAAACCAAAGGGTGACAGGAGATCAACTGAAATTTCTTTGGTTATTCGACAAACCCTAGAAGAATGCATATTAACACATTTAATGCCCCGTTCCCAGATAGATATTTATGTCCAAGTTCTCCAAGCTGATGGAGGAACAAGGTCTGCTTGTATAAATGCTGCAACTCTGGCCCTAGCTGATGCTGGGATTCCCATGCGTGATCTTGTAACTTCCTGTAGTGCTGGATACCTCAATAGCACGCCTCTACTTGATTTGAACTATGTAGAAGACAGTGCTGGGGGTCCTGATGTAACCGTAGGAATTCTGCCGAAGTTGGATAAAGTGACGCTTCTTCAGATGGATTCTAAACTACCAATAGACATTTTGGAAAATGTTATACAACTTGCTACAGAAGGTTGCAAAGCAATTGCAAACTACATACGAGAAATTTTGCTGGAGAACACAAAGGAACTTGAGTATCGACGGGGTTCATAGTCTTATAGAAGTAATATTTCCTTTTTGCTTGTTTCTTTTTCTTGGTTTATTTCATGTTATGCATATAACTTGATACTATGGTGGTTGTTGCTGATGGTAGTTCTTTTCATGCACTGCTAGTTTATTTGTTTTTTATTGTTTTTTACTTTTTAATTTCTTWAGGGTTTAAGAATTTCTAAATAAGTTTAATTTACCTGAAAATGTTTGAAGGTAAGAAAAACG
TARGET=42,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01025 Ps_contig_mira-and-tgicl-ass_franssen_9572
SEQUENCE=CGTTTMWAAAAAWWATGAAAATTATTCTTTATAAAAAACAAATGAATCTAAAAATGCTTTTGCTAACAATCACAATCCCCAAAACAGCTAGAATGATTACAAACAAAACACAATCTAAGAAACAAAATGAATCTTACAAACCAAAAAAATGGACATGTATTATTCTTCAACACAAACAAAACTAACAAAAACCAATCTTACTACTTATGTAACTAGTAATGAATCTCTTTAAAAGCATTGGTTATTCTCAGCCTAGTATAAACAGCACGCCACCCTGCATCAGTAGGATGAACCAAATCCCAAAAGAAAGCAGATTTAGGATTCTCACAAACCGTATATTTCTTAACCTTCTCCACAACACTTCCACAAAAATACTTACTACTCACTCCAACACAACAAGGTTCCAACTCGTTCTTAATATTGTGTGTGGATGGATGCTTCAACACCGACATGAAACTATCGTATAGATCAAGAACTATGAAGGGTAATGAAGAATTATCGTTGAGTTGTTGGTTTAACTTTGTGACAGCTTCGGTTAAGAGGGTGTTATGGTAAGCTACGAGGAGATTGTTGGATGTTTCATTGCATTCTTTGAATGAAGATGAAGCTGTTACCGAAGGTAGACATCCAACTGGTTGTAGATTTGATATAATCACTTTTTTCACTCCTAATTCTTTGATACGAATCACGTTTCTGATTGTTTGATTAACCACCGATAATATGAATGATGGTAAACCCTGAATAGAGCCATTTGTGACAGTGTAACGAGTGTAGTCATTACCAGCTACAGAAACAAGAGCAACTGATTTTCTAATATCTGAGGCTGTGAATACTTTGTCTTGTATTGCCTTTTGAAAAAAATTGATCTGAGTTGTCATGTTTGGACCTGGATTCAATGTCTCAAACACACCAGTACCACCAAATGCAAAGCTCATCCCATTTTTCAAATGATGTGGTGCCAAATGTTTTCTTATTCTGTATGATGCTGGTGATTTCACTTTCAAATACTTGGCAATGTAGTCGGTTAGGACCCTGCCGTCGGAGAAACGACCGGCGGGCTTGCCGGGAAAGGTGACACCATAGGGATGTTTCCACGAAGGAGCAAAACCTCTTTTGATGTTTCCTGTGTCAGCATATGAATCTCCAAACACAAACAATTTTGTTATAGGCTCTTTATGATGATGGTGGTGACGTTGAATATCAACCTCACCTTGTACAAATCTTTGTCCTAAGAGATGTAGAATAATGAAAATGAAAGAGAGGAAAAAGGGAAAGAAATCCATGAATGGGTTTTGCTACGTGGTTAGTAGTAGCTAAAGGTTAGAGAGAGAGAGAGTGAAGTGATGAATGAAGGTTTTGAGATTTGAAGGGAACCCCCGC
TARGET=1323,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01026 Ps_contig_mira-and-tgicl-ass_franssen_9573
SEQUENCE=GAAGGGAAGGTAGGCGAAGAAGCGGGAGGGAGGGATACGCNGGAGANGAAGATCGATATGCGAGAATCCGTTATGCATGTGATGGTGGGGGTTTTTGGGTCGGTGTTCCCACGATGAAAAAAGTAGAAAGGTGTTAATGGAGCTGGTTATATAGATTTTAAGGTTAACAGAGTTAAATTCTGTTAAGTTCTTAGATTGAAAATCCGTTTGTAGTTAAACCTCTGTTTGGATCTGTTAAGTGAATTTGTTAGTTTTTTGTTATTCTTCCTTTTTCTTCTCTCTTTTTATTTTTGTTTCTGTATGAACTTCTTGCATTTTTTT
TARGET=24,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.4118
PRIMER_LEFT_PENALTY=2.064680
PRIMER_RIGHT_PENALTY=0.347129
PRIMER_LEFT_SEQUENCE=AAGGGAAGGTAGGCGAAGAAG
PRIMER_RIGHT_SEQUENCE=CCAGCTCCATTAACACCTTTCT
PRIMER_LEFT=1,21
PRIMER_RIGHT=145,22
PRIMER_LEFT_TM=61.065
PRIMER_RIGHT_TM=59.653
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=2.5048
PRIMER_LEFT_1_PENALTY=2.157667
PRIMER_RIGHT_1_PENALTY=0.347129
PRIMER_LEFT_1_SEQUENCE=AGGGAAGGTAGGCGAAGAAG
PRIMER_RIGHT_1_SEQUENCE=CCAGCTCCATTAACACCTTTCT
PRIMER_LEFT_1=2,20
PRIMER_RIGHT_1=145,22
PRIMER_LEFT_1_TM=59.842
PRIMER_RIGHT_1_TM=59.653
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=2.5385
PRIMER_LEFT_2_PENALTY=2.064680
PRIMER_RIGHT_2_PENALTY=0.473868
PRIMER_LEFT_2_SEQUENCE=AAGGGAAGGTAGGCGAAGAAG
PRIMER_RIGHT_2_SEQUENCE=ACCAGCTCCATTAACACCTTTC
PRIMER_LEFT_2=1,21
PRIMER_RIGHT_2=146,22
PRIMER_LEFT_2_TM=61.065
PRIMER_RIGHT_2_TM=59.526
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=2.6315
PRIMER_LEFT_3_PENALTY=2.157667
PRIMER_RIGHT_3_PENALTY=0.473868
PRIMER_LEFT_3_SEQUENCE=AGGGAAGGTAGGCGAAGAAG
PRIMER_RIGHT_3_SEQUENCE=ACCAGCTCCATTAACACCTTTC
PRIMER_LEFT_3=2,20
PRIMER_RIGHT_3=146,22
PRIMER_LEFT_3_TM=59.842
PRIMER_RIGHT_3_TM=59.526
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=2.6742
PRIMER_LEFT_4_PENALTY=2.200343
PRIMER_RIGHT_4_PENALTY=0.473868
PRIMER_LEFT_4_SEQUENCE=AAGGGAAGGTAGGCGAAGAA
PRIMER_RIGHT_4_SEQUENCE=ACCAGCTCCATTAACACCTTTC
PRIMER_LEFT_4=1,20
PRIMER_RIGHT_4=146,22
PRIMER_LEFT_4_TM=60.200
PRIMER_RIGHT_4_TM=59.526
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_01027 Ps_contig_mira-and-tgicl-ass_franssen_9580
SEQUENCE=TTGTTGGGCGTTCATGTAATTTGGTTGAACCATCTTGTTTTGTAGTTTTTCTTTGAAACTGGTGTTGAATGAAGTTGAAGAGTCTCAAGATCAGAGAGATTGATTGAGTTTGATTTCTTCATTTTCTTCTTCATATCAAAAAGTTTACTTTTTTTCATCTTGGTTTTGATGAGGAGAAGATGAAGAAGATAGTTTCATGTTTGTTGAAAATTTCTCTGATTTAACATGTTGTGTTTGGATACTAAGCTTGTTTGGGACCTTTCTTTGAACCATTTTTTAAGAAAGGGAGGAAGGTTTTGTTGTGGTTTTGGTTTTCATGTGAGGAAGTTTAGGAAGAAAAGGTTTTGAAAGCTTGAAACACAAACAACCATAGAGAAAGAGAGAGAGAGAGATTCTCTTCTTTTAGTTTTGGTTATTCTTTTTGTTTATTTTCAATAGTSGTTTACYCYGMRYTG
TARGET=378,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01028 Ps_contig_mira-and-tgicl-ass_franssen_9587
SEQUENCE=GATCTTTGTCTTTCAGAACATTATCAATCTTTGTCTTTTGCCGACACCCTTTCTTGTTTGCTACATATTACATGATTGACTCACCCAAAATATATCATCAAAGCTTTTCAAAAAAGTTAAATTTTGCTTCTGCTTCTTGACATGTCTGAACTCCTTTGCAAAGATTTAAGAAGAGAGAATTCAGAGGAAGATAAGTGGAAAAAAATGGGGAGTCTTAAAAAGAAAGCAATAAATGCTTCTTGCAAGTTAAGGYATTCTACGTTAAGAAGAAGAAGAAGTAGACAAAAGGTGGAAGTAGACACACTCACTTCATGAG
TARGET=264,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01029 Ps_contig_mira-and-tgicl-ass_franssen_9595
SEQUENCE=AAACCAACGATCTTCACGAAACAGAAAACAATAAGACAATAAAAGAAGAGAAATCAGTGTCTCTCTTCACTACCACACGCTTCCTCCGTCAAGCCGCTCACCAAACACCATTAAACCCCTAAATCCGCCGTCAACACCCAAAACGGCTTCACCATCAAACATCACACGAAACTCTTACTCCGCCGTCAGTCATCTTCAACCCTCTTCAAACCCTAAATCCGCCGTCAACACCCAAAACGGCTCCACCACCAACTCGCACGATTTCCTTCACGGTTGTTCCTTTTGTTGGCTCGTGAAAGTGGTTTGAGGAGGTAGCCTCTGGTTATTTTGACGCGGTTCCGGCTCAGATTCAAGCAACTCACAACCGCTGCCTCACGCCCATCATCATCATCGTCGTCGCCGCCCCTCCATAACTCACAGTTCCTTTCCGATCTGTACACGGGTGAGATCCGGGAAAGCTATCGGGTTATGCGTTAATGTTTGTGGATTTGGCAGAGATTCAGTTTTTATTAGTGTTTGTTACTGAGGCGTTTGCTGGAAAGGCAATGGATACGCATCAAATCTTCAGCATCAACATGTCGATTCCATCTACCTTGAGCACAATATTGGCAATACAGCAATGACGCCACTGATAACAAAACAGGGCAGCAACATTCGATGCGGTAACATTTCAGTGTTTGTGTTTATTTTRTTTGTTTCGTRGCGTACAWGTTTRKTTATTGAACG
TARGET=380,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01030 Ps_contig_mira-and-tgicl-ass_franssen_9596
SEQUENCE=GTCGGSGAKGGGGATTCATCTCTCTCTCCCCACATCTTCTTTTGGTTTTTTCCCTCACGCTAACTACATAACCCTTTCATTCTCTCACTCACTCTCCAGCTACCACCTAACAACCTACTACACTAAAAATTCTCACAACTTCACTCAGAAATCCATCTTCGTGGCCTCGCCGTTAGCCCACACAAACCCTCCATCCGACTCCACTCCATTCACCATCGCACACCCTTCATCACTCAACTACGCACTCACAAAACCACGGATTACCTTAACAAACGAAACAGAAAACATAAGCAATAAAAGAGAGAAATCAGTGTCTCTCTTCACTACCACACGCTTCCTCCGCCTCCACTAAGTTCTTCAACCACCATTAAACCCCTAAATCCGCCGTCAACACCCAAAACGGCTTCACCATCAAACATCACACGAAACTCTTACTCCG
TARGET=19,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01031 Ps_contig_mira-and-tgicl-ass_franssen_9601
SEQUENCE=CAAATCCCTCCAATCGCCATGTTTGTTTGTTGCTAGCCATTACAATGAAACAAATAAGAGAGAAAAGCAAAGCGATCCCTCTCAATCCCAAGGATCCTCTCTTTATCAAGTCTTCCCTCTTCCACCGCTTCAATATCCCTGCTATGCCTTCGGTCGGCGGCGGTGGGTTTTCCCGATCAGTCACATCTGGCGCCGGTGGTGGAGTCTGAATGTGTATCCTCGGGGGAGATGAATCTTCTTCGATGTTGGACATTTTCTGAGGATGTTTTTTACTTTTTTAATGTTAAAAATTGTAGTATTTAGATTCAGATATGRAGGATTGAATTGAAAATTGAAAAGAAGAATAGAACAAGAAAGAA
TARGET=20,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01032 Ps_contig_mira-and-tgicl-ass_franssen_9610
SEQUENCE=TTYTWCCAAAATAAATATTTATTATAGTTATAGGTTCTTAATATCATCCATCATATACATACATACATAGAAAGACACAGATGCAGATGCACCTATGCTATTGTTGTTTCAGTAGTAGAGGATGGCTGCAAAAGTGTAGAAGATGAACCCAGAGGCTCATGCACTACAGAATTTGAAGAAACTGATATAGGACTTCCTTCCAACTGCAACATGAGACTCCCCAATAATTTTCAGTTAATACTTTCTTGAACTCATCTTCTTTCACCAACTTCGCATCATCTGCACTCACCATCTTAAACACTTCACCGATAACCTTATCCATCTCTTGAACTGCGCCAAAAGGAGGTAAAGCAGCGGATGGAGCCACCACATCCAGCGCCACTCGGAGATAATCCTTTGACATTTTTCCATTACGATCTTTAGGGATAGTCTCTATAGCTGAGTTCAAAATCTTGTCTAATTCAAACTTATTTGATAATAGTCTCTTAACACCACTTCCATCAAATTTGCTTTCACTATGAGCAACTATGACAGGTTGCTCCCGAAGACGATTCGCTACGAGCAATGCCACTCTCTTAAATTCTTCAATAAATATCTCTTGAGAAACTGGCTTATTCAAACTAGATCCACGTCCAGAAAGCAGTGCTGGTTCCACAATGTTGTTAATAACCCAAGGATCAGAAGTAGGAGGAATCCCTTGATCAACATTGAGTTTGCTGAGAGCTTGAATTATAAGATCATGAAGAGATGCGTGAGGTGATTCAATTTGCGAGAAAATGGATACGATTTCGGGTTCATAAACTGAACCATTCAGAAACTCGAGAAGATCCTCGCCATCAATCCGAAGTATCACAATCGGGTCTCGTTTGAGTCCAGCAGCCATGCCCAACAGAATGTCTGAAAGAACCTCTTTGAACTCAGTCTTGCTCACTTTTTCATGCTTTCCATGTGTGAATTCATTCAAAACCTCAGAATAGATGTGATCAGAATCAGGATTAGTGCCTTTAGGAGGTAAACCAAGAGCAGCACCAATATCAGAAACAGCAGGTTTGAGTTCGTTCACAGAAAGTTTACCATCTCTATCTCGATCCAACTCTTGAAACTTATGGTTCACSAAGKTGCTAGAAAACNTTCTTTGTTAACCAACTAACTCATAATATAC
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01033 Ps_contig_mira-and-tgicl-ass_franssen_9624
SEQUENCE=TCTTATGGACTAGTCCATGGTTGATTGGAGTAGGTTTATGTTATGATTATGGTTGACTAGGTTTTTGATATTGTTATATTTATCTTAATCAAGAGTTATTAGGACTAGTTTGAATTTGATTTGATTAATTAACGTTTGTGTGTATGAGAAGAAGGTGCAATAAAGATAGTTTATAGGATAATAATAATAATAGCAAGGAAAAAGAAACAAATAAGTTGCATTGTTTGTTGTAACTTAGATGGTGGGAGGAGAGTTATCTTGTGAGTTGTGATTA
TARGET=178,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2307
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=1.121175
PRIMER_LEFT_SEQUENCE=CCATGGTTGATTGGAGTAGGTT
PRIMER_RIGHT_SEQUENCE=TCCTCCCACCATCTAAGTTACAA
PRIMER_LEFT=14,22
PRIMER_RIGHT=249,23
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.879
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=1.3727
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=1.263235
PRIMER_LEFT_1_SEQUENCE=CCATGGTTGATTGGAGTAGGTT
PRIMER_RIGHT_1_SEQUENCE=CTCTCCTCCCACCATCTAAGTT
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=252,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=58.737
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=1.7311
PRIMER_LEFT_2_PENALTY=0.609876
PRIMER_RIGHT_2_PENALTY=1.121175
PRIMER_LEFT_2_SEQUENCE=GTCCATGGTTGATTGGAGTAGG
PRIMER_RIGHT_2_SEQUENCE=TCCTCCCACCATCTAAGTTACAA
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=249,23
PRIMER_LEFT_2_TM=60.610
PRIMER_RIGHT_2_TM=59.879
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=1.7311
PRIMER_LEFT_3_PENALTY=0.609876
PRIMER_RIGHT_3_PENALTY=1.121175
PRIMER_LEFT_3_SEQUENCE=TCCATGGTTGATTGGAGTAGGT
PRIMER_RIGHT_3_SEQUENCE=TCCTCCCACCATCTAAGTTACAA
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=249,23
PRIMER_LEFT_3_TM=60.610
PRIMER_RIGHT_3_TM=59.879
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=1.8731
PRIMER_LEFT_4_PENALTY=0.609876
PRIMER_RIGHT_4_PENALTY=1.263235
PRIMER_LEFT_4_SEQUENCE=TCCATGGTTGATTGGAGTAGGT
PRIMER_RIGHT_4_SEQUENCE=CTCTCCTCCCACCATCTAAGTT
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=60.610
PRIMER_RIGHT_4_TM=58.737
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_01034 Ps_contig_mira-and-tgicl-ass_franssen_9626
SEQUENCE=TTAACTGCTTTGTTTGGATTGATGGAAAAAAAAATTGCTTATKAYYWHGAAGGCCCCAACGTCACTTTGCAACGCGTGTGGGATTAGACAAAGGAAGGCGAGGCGCGCGATGGCATTGGCTGCCGCGTCTGGAAACGGAACAACAATTGCTGATCACGAGACATCTTGTGCGAAGAAGAGAAAAAAGCTGCAGAAGAAGAAGGAGAATAAGTCCAAAATTGAGTTTGAATGTTCAGCGCATATGAAAAAGAAACGCAAGATGGAAGCAACAAAAGCGTCTCAAATTAGTGGAAACAAGTTCATCAGTTTCGAGGATTTGAGATTAAGCTTGAGCCAGAATTTGTCTGTTCAACAAGTTTTTCCTCAGGATGAGAAGGAAGCTGCGATCCTTCTCATGGCTTTATCTTATGGACTAGTCCATGGTTGATTGGAGAGGTTTATGTTATGATTATGGTTGACTAGGTTTTTGATATTGTTATATTTATCTTAATCAAGAGTTATTAGGACTAGTTTGAATTTGATTTGATTAATTAACGTTTGTGTGTATGAGAAGAAGGTGCAATAAAGATAGTTTATAGGATAATAATAATAGCAAGGAAAAAGAACAAATAAGTTGCATTGTTTGTTGTAACTTGATGGTGGGAGAGAGTTATCTTGTGAGTTGTGATCTTAATTATATAAATGTTTGATTTGAAAATTARMATGTGAGTTGTAATCTTATAAGTACT
TARGET=580,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01035 Ps_contig_mira-and-tgicl-ass_franssen_9629
SEQUENCE=CGACTCAATTGATTTCTTCACAACCAAATTTTCCTTTTATCCTTTTCAAGCTCTTCAACCCTTCTTTCTTTCTCAATCTTTTCCTCCAACAGACTCCCAGCTTCCAACCTAATTCTCTCCACATTCCGCTTCTCGTTCTCAAGACTCAACACAAGGTCATTCACCTTCTCGTTCTGAAGATCCACCTCGTACTTGGTCTCATCCCTCTCCTCAACAACCTTATCGAAACGAAACCCTAATTCAGCAATATGAGCCCTAGACAAAAAACGAAAAAGACCGAGT
TARGET=62,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1505
PRIMER_LEFT_PENALTY=1.106446
PRIMER_RIGHT_PENALTY=0.044097
PRIMER_LEFT_SEQUENCE=ATCCTTTTCAAGCTCTTCAACC
PRIMER_RIGHT_SEQUENCE=GGCTCATATTGCTGAATTAGGG
PRIMER_LEFT=38,22
PRIMER_RIGHT=254,22
PRIMER_LEFT_TM=58.894
PRIMER_RIGHT_TM=59.956
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=1.1505
PRIMER_LEFT_1_PENALTY=1.106446
PRIMER_RIGHT_1_PENALTY=0.044097
PRIMER_LEFT_1_SEQUENCE=ATCCTTTTCAAGCTCTTCAACC
PRIMER_RIGHT_1_SEQUENCE=GGGCTCATATTGCTGAATTAGG
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=255,22
PRIMER_LEFT_1_TM=58.894
PRIMER_RIGHT_1_TM=59.956
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=218
PRIMER_PAIR_PENALTY_2=1.2095
PRIMER_LEFT_2_PENALTY=1.086266
PRIMER_RIGHT_2_PENALTY=0.123278
PRIMER_LEFT_2_SEQUENCE=CCTTTTCAAGCTCTTCAACCCT
PRIMER_RIGHT_2_SEQUENCE=CGATAAGGTTGTTGAGGAGAGG
PRIMER_LEFT_2=40,22
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=61.086
PRIMER_RIGHT_2_TM=60.123
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=1.2180
PRIMER_LEFT_3_PENALTY=1.086266
PRIMER_RIGHT_3_PENALTY=0.131699
PRIMER_LEFT_3_SEQUENCE=CCTTTTCAAGCTCTTCAACCCT
PRIMER_RIGHT_3_SEQUENCE=AGAGGGATGAGACCAAGTACGA
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=207,22
PRIMER_LEFT_3_TM=61.086
PRIMER_RIGHT_3_TM=60.132
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=1.2180
PRIMER_LEFT_4_PENALTY=1.086266
PRIMER_RIGHT_4_PENALTY=0.131699
PRIMER_LEFT_4_SEQUENCE=CCTTTTCAAGCTCTTCAACCCT
PRIMER_RIGHT_4_SEQUENCE=GAGAGGGATGAGACCAAGTACG
PRIMER_LEFT_4=40,22
PRIMER_RIGHT_4=208,22
PRIMER_LEFT_4_TM=61.086
PRIMER_RIGHT_4_TM=60.132
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=169
=
PRIMER_SEQUENCE_ID=SSR_01036 Ps_contig_mira-and-tgicl-ass_franssen_9632
SEQUENCE=TAGAATAACTCCAGCACTCCAGATATCTACTTCCGGGCCATAATTTCTCTTCAATACCTCCGGAGCCATGTAATATGGACTTCCAACTATCTCATTAAATCTTTCACCTGGTTTAAAGAACACTGACAAACCAAAATCTATAGCTTTCAAAGCTGCTGTTTCCTTTTTATTCGCAAACAAAAAATTCTCCGGCTTGAGATCTCTATGCATCACACCATGTTTATGGCACATCTGAACAACTTCAACGATAGTTTTGGTAACAGCCGCGGCGGCTCGTTCAGTATAATGACCCCTGGCAACAATCCGATCAAAAAGCTCCCCACCTTCACAAAGCTCCATAACAAGATGAACAGCATTATCATCCTCATAAGTATCTTTCAACGTAACAATATTATTATGCGGAGGCATATGCTTCATGATTTCAACTTCTCTCCTAACATCTTCAATATCAATAGCAGTTCTAAGCTTCTTCTTCGAAATCGACTTGCACGCCAGTTCCTCTCTGGTTTCCTTGTCTGTGCAAAGATACGTTACGCCGAATTCACCTCTACCGAGTTCACGACCTAGCTCGTAACGTGTCTCGATTTCGCGGCCGGTTGGATCCTTCAAAACGGTGAGTTTTGAGCTTGAGGTTTTGTTGTTGTTGAAACCGTAATCGATGGCGAAGGGATTCTCCTTTTTAGCCTTTTTGTTTTTCTTTGTTGGTTGTGTCTTCTCCTGTTACAGCAGGGGTAGCACAACAATTTCCCATGGTGATTTTTAGAGATTTATCCTAAAAATTATTTAAGATCTGGGTTTGAAATTTGAATTGGATCTAGCTATCTGATATAGAGAAGAGAATGAGAAGGGTATAATGGGAAAAGGGAAATTAAAAGTAGTTAGGTTTTTTT
TARGET=635,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2130
PRIMER_LEFT_PENALTY=0.027715
PRIMER_RIGHT_PENALTY=0.185267
PRIMER_LEFT_SEQUENCE=GTTACGCCGAATTCACCTCTAC
PRIMER_RIGHT_SEQUENCE=CAGGAGAAGACACAACCAACAA
PRIMER_LEFT=529,22
PRIMER_RIGHT=719,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=60.185
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.2882
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.082963
PRIMER_LEFT_1_SEQUENCE=GGTGAGTTTTGAGCTTGAGGTT
PRIMER_RIGHT_1_SEQUENCE=CCCTTTTCCCATTATACCCTTC
PRIMER_LEFT_1=612,22
PRIMER_RIGHT_1=864,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=59.917
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.2882
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.082963
PRIMER_LEFT_2_SEQUENCE=GGTGAGTTTTGAGCTTGAGGTT
PRIMER_RIGHT_2_SEQUENCE=TCCCTTTTCCCATTATACCCTT
PRIMER_LEFT_2=612,22
PRIMER_RIGHT_2=865,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=59.917
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=0.2882
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.082963
PRIMER_LEFT_3_SEQUENCE=GGTGAGTTTTGAGCTTGAGGTT
PRIMER_RIGHT_3_SEQUENCE=TTCCCTTTTCCCATTATACCCT
PRIMER_LEFT_3=612,22
PRIMER_RIGHT_3=866,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=59.917
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.3156
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.185267
PRIMER_LEFT_4_SEQUENCE=CGAATTCACCTCTACCGAGTTC
PRIMER_RIGHT_4_SEQUENCE=CAGGAGAAGACACAACCAACAA
PRIMER_LEFT_4=536,22
PRIMER_RIGHT_4=719,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=60.185
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_01037 Ps_contig_mira-and-tgicl-ass_franssen_9640
SEQUENCE=GACACACACACATACAACCCACAGTAATCATCATGTCCTCTTCCTTATTCCTCCCTTCATTATTATTATTCTCTCCCTTTCATCATCATCACTTCATCTTTTACCTCCCACCAACCGCACAAAACCCATCATCTATCTCTAATAAGGTCGCTACCATAACAATTACAAGAAAGAAAAAAGATAGAGATAAAGGTTAGAAGATGAAAGAAGATGGCTTCATACAAGAAGAAGAAACCCTAGCTACCAAGAATTACTATTTTTCTTTTGATTTCAAGGGTAGTGATTTGATATATCTTCTTCTTAATTAATTTCATATGGTTATCATCATCATCATGCTACTTTCTTCTCTTCTCGGGGAATTGAAGCTTTATGGTCCGATTATCTGCTAGATCAAGCTAAGTTTATATTCGCYACCTTTCTTTCTCTACTTATTTATTTTATYKTTTTCGG
TARGET=2,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01038 Ps_contig_mira-and-tgicl-ass_franssen_9640
SEQUENCE=GACACACACACATACAACCCACAGTAATCATCATGTCCTCTTCCTTATTCCTCCCTTCATTATTATTATTCTCTCCCTTTCATCATCATCACTTCATCTTTTACCTCCCACCAACCGCACAAAACCCATCATCTATCTCTAATAAGGTCGCTACCATAACAATTACAAGAAAGAAAAAAGATAGAGATAAAGGTTAGAAGATGAAAGAAGATGGCTTCATACAAGAAGAAGAAACCCTAGCTACCAAGAATTACTATTTTTCTTTTGATTTCAAGGGTAGTGATTTGATATATCTTCTTCTTAATTAATTTCATATGGTTATCATCATCATCATGCTACTTTCTTCTCTTCTCGGGGAATTGAAGCTTTATGGTCCGATTATCTGCTAGATCAAGCTAAGTTTATATTCGCYACCTTTCTTTCTCTACTTATTTATTTTATYKTTTTCGG
TARGET=59,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01039 Ps_contig_mira-and-tgicl-ass_franssen_9640
SEQUENCE=GACACACACACATACAACCCACAGTAATCATCATGTCCTCTTCCTTATTCCTCCCTTCATTATTATTATTCTCTCCCTTTCATCATCATCACTTCATCTTTTACCTCCCACCAACCGCACAAAACCCATCATCTATCTCTAATAAGGTCGCTACCATAACAATTACAAGAAAGAAAAAAGATAGAGATAAAGGTTAGAAGATGAAAGAAGATGGCTTCATACAAGAAGAAGAAACCCTAGCTACCAAGAATTACTATTTTTCTTTTGATTTCAAGGGTAGTGATTTGATATATCTTCTTCTTAATTAATTTCATATGGTTATCATCATCATCATGCTACTTTCTTCTCTTCTCGGGGAATTGAAGCTTTATGGTCCGATTATCTGCTAGATCAAGCTAAGTTTATATTCGCYACCTTTCTTTCTCTACTTATTTATTTTATYKTTTTCGG
TARGET=80,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01040 Ps_contig_mira-and-tgicl-ass_franssen_9640
SEQUENCE=GACACACACACATACAACCCACAGTAATCATCATGTCCTCTTCCTTATTCCTCCCTTCATTATTATTATTCTCTCCCTTTCATCATCATCACTTCATCTTTTACCTCCCACCAACCGCACAAAACCCATCATCTATCTCTAATAAGGTCGCTACCATAACAATTACAAGAAAGAAAAAAGATAGAGATAAAGGTTAGAAGATGAAAGAAGATGGCTTCATACAAGAAGAAGAAACCCTAGCTACCAAGAATTACTATTTTTCTTTTGATTTCAAGGGTAGTGATTTGATATATCTTCTTCTTAATTAATTTCATATGGTTATCATCATCATCATGCTACTTTCTTCTCTTCTCGGGGAATTGAAGCTTTATGGTCCGATTATCTGCTAGATCAAGCTAAGTTTATATTCGCYACCTTTCTTTCTCTACTTATTTATTTTATYKTTTTCGG
TARGET=321,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01041 Ps_contig_mira-and-tgicl-ass_franssen_9648
SEQUENCE=GTTCGGGTTCTTACCTACATAAAARWYCRRKWCAAGTATTCATCTCCACCACCTCCAACCCACCACGTTTACCCTCACCCAATCTACCACTCACCACCACCACCAAAGAAACCATACAAATATTCATCACCTCCACCACCAGTCCCAACTCCCTACGTACCTCACCCAGTTTACTCCCCACCAAAGAAGCCATACAAATACTCCTCTCCTCCACCACCGGTCCACCCTCCATACGTTCCTCATCCAGTGTACTCACCACCAAAGAAACCATACAAATATTCATCACCTCCACCACCAGTCCCAACTCCCTACGTTCCTCATCCAGTGTACTCACCACCAAAGAAACCATATAAGTACTCATCACCTCCACCACCAGTCCCAACTCCCTACGTTCCTCACCCAGTTTACTCACCACCAAAGAAACCATACAAATACTCATCTCCTCCACCACCAGTCCCCACTCCATACGTTCCTCACCCAGTTTACTCACCACCTAAAAAGCCATACAAATACTCATCTCCACCACCACCGGTTCCCTCACCCTACATTCCTCACCCAGTTTACTCACCACCAAAAAAGCCATATAAATACTCATCTCCACCACCTCCAGTCCCCACACCCTACGTTCCTCACCCAGTTTACTCACCACCCAAGAAGCCATACAAGTACGCTTCTCCTCCTCCTCCACCAGTTCACACTTATCCTCCATACATTCCTCACCCAGTTCACCACTCTCCTCCACCAACTCCAACCAAGAAGCCTTACCTCTATGTTTCTCCTCCCCCTCCTTACCACTCCTAGATAGTGGTTTGCCATAGCTCTCTATGATATATGAGTGAAGAATATTTGAAAATAAAGAAAGCGATGGAACGAAGGAAGAAGCATTGTTCGTAATAACAGAAGAAAAAGAAGAAAAGGAAGCAAAGTTTCCGCATTTTGTCTTCATCCAAAAAGTGTTAATTTATTTTCTGTTTCTATGATAGGATTGCTATTGCGTTTTTCTAATGTGCTTTTGTTTAGTTTATTTCCATGAAAATCGCTTTTAATATTTGACAGTTGTAAAGCAGCACTGTTCTGCTATAGTACATGCAAATGTGGATGATGTGTAGTCTTCAACCTGCTTCTTTTGCAGGGGTTGTAAGACATTTTATATATATTATATCAATATCAATTACTATATKAAAGTTAAT
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01042 Ps_contig_mira-and-tgicl-ass_franssen_9648
SEQUENCE=GTTCGGGTTCTTACCTACATAAAARWYCRRKWCAAGTATTCATCTCCACCACCTCCAACCCACCACGTTTACCCTCACCCAATCTACCACTCACCACCACCACCAAAGAAACCATACAAATATTCATCACCTCCACCACCAGTCCCAACTCCCTACGTACCTCACCCAGTTTACTCCCCACCAAAGAAGCCATACAAATACTCCTCTCCTCCACCACCGGTCCACCCTCCATACGTTCCTCATCCAGTGTACTCACCACCAAAGAAACCATACAAATATTCATCACCTCCACCACCAGTCCCAACTCCCTACGTTCCTCATCCAGTGTACTCACCACCAAAGAAACCATATAAGTACTCATCACCTCCACCACCAGTCCCAACTCCCTACGTTCCTCACCCAGTTTACTCACCACCAAAGAAACCATACAAATACTCATCTCCTCCACCACCAGTCCCCACTCCATACGTTCCTCACCCAGTTTACTCACCACCTAAAAAGCCATACAAATACTCATCTCCACCACCACCGGTTCCCTCACCCTACATTCCTCACCCAGTTTACTCACCACCAAAAAAGCCATATAAATACTCATCTCCACCACCTCCAGTCCCCACACCCTACGTTCCTCACCCAGTTTACTCACCACCCAAGAAGCCATACAAGTACGCTTCTCCTCCTCCTCCACCAGTTCACACTTATCCTCCATACATTCCTCACCCAGTTCACCACTCTCCTCCACCAACTCCAACCAAGAAGCCTTACCTCTATGTTTCTCCTCCCCCTCCTTACCACTCCTAGATAGTGGTTTGCCATAGCTCTCTATGATATATGAGTGAAGAATATTTGAAAATAAAGAAAGCGATGGAACGAAGGAAGAAGCATTGTTCGTAATAACAGAAGAAAAAGAAGAAAAGGAAGCAAAGTTTCCGCATTTTGTCTTCATCCAAAAAGTGTTAATTTATTTTCTGTTTCTATGATAGGATTGCTATTGCGTTTTTCTAATGTGCTTTTGTTTAGTTTATTTCCATGAAAATCGCTTTTAATATTTGACAGTTGTAAAGCAGCACTGTTCTGCTATAGTACATGCAAATGTGGATGATGTGTAGTCTTCAACCTGCTTCTTTTGCAGGGGTTGTAAGACATTTTATATATATTATATCAATATCAATTACTATATKAAAGTTAAT
TARGET=674,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01043 Ps_contig_mira-and-tgicl-ass_franssen_9663
SEQUENCE=GAACCTTGAAATCTCCATCTATCAATATATAACAACCTCCTTCCACTCTTCTTGCTCCACCCAAAATACTACAAAACCAGTAATCAAATTTCTCTGTCAACTAAAAAGCAACAAAATCAGTTATTAGATTTCAAGTAATCATTAACACAGAAGAAGATGAGTTCAACAAGCAGAGCCTGGGCAGCAGCAGCCAGTGTTGCAGTCGTGGAAGCATTGAAGGATCAAGGTATATGCAGATGGAATCATACRCTAAAATCACTTTCAAATCATGTAAAAAAACAATGTCAGATCATTTTCTCAAGCAAAGAAACTCYCATCCTCCACTTCTTCTTCTTCAGTTATGGTTTCTAGTAATAGACAAAAAGAGATGGCAAAGCAAT
TARGET=324,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01044 Ps_contig_mira-and-tgicl-ass_franssen_9665
SEQUENCE=AWTGGGCGGCAGCAGCCAGTGTTGCAGTGGTGGAAGCATTGAAGGATCAAGGTATATGCAGATGGAATCATACACTAAAGTCACTTCAAAATCATGTTAAAAACAATGTCAGATCATTTTCTCAAGCAAAGAAACTCTCATCCTCCACTTCTTCTTCTTCAGTTATGGTTTCTACTAATAGAACAAAAAAGATGGACAAAGCAATCAGAAGAATCTTTAGG
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01045 Ps_contig_mira-and-tgicl-ass_franssen_9670
SEQUENCE=CGGGGGGNGGTTTACGGACTGAGATAGATGCAGAACAGGGGAAGGTGACGGTTTCCGGGAATGTGGACCCAAACATTCTCATCAAGAAGCTTGCAAAATCGGGAAAACACGCAGAGTTATGGAGTGTACCCAAACCAAACAACAACAACAACAACAACAACAACCAGAACAATGTTGTTAATCAGTTAAAGAACATGCAAATTGATAATGGTAAAGGTGGTGGTGGAAACAAGGACAACAACAAAGGTCAGAATCAGAACCAGAAGGGTGGGAATAGTAACCAGCCTAAAGGTGTTCCACCAGGGTTAAATCCTCAGCAGGTGCAACAACTTCAGCTTCAGCAACAGTTGCAGCAACTGACAAA
TARGET=138,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1819
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=GATAGATGCAGAACAGGGGAAG
PRIMER_RIGHT_SEQUENCE=TTGTTGTTGTCCTTGTTTCCAC
PRIMER_LEFT=22,22
PRIMER_RIGHT=243,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.2242
PRIMER_LEFT_1_PENALTY=0.095574
PRIMER_RIGHT_1_PENALTY=0.128591
PRIMER_LEFT_1_SEQUENCE=GATAGATGCAGAACAGGGGAAG
PRIMER_RIGHT_1_SEQUENCE=CCACCACCACCTTTACCATTAT
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=225,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.871
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.2242
PRIMER_LEFT_2_PENALTY=0.095574
PRIMER_RIGHT_2_PENALTY=0.128591
PRIMER_LEFT_2_SEQUENCE=AGATAGATGCAGAACAGGGGAA
PRIMER_RIGHT_2_SEQUENCE=CCACCACCACCTTTACCATTAT
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=225,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=59.871
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.4220
PRIMER_LEFT_3_PENALTY=0.095574
PRIMER_RIGHT_3_PENALTY=0.326376
PRIMER_LEFT_3_SEQUENCE=GATAGATGCAGAACAGGGGAAG
PRIMER_RIGHT_3_SEQUENCE=CTGACCTTTGTTGTTGTCCTTG
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=250,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.674
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=0.4220
PRIMER_LEFT_4_PENALTY=0.095574
PRIMER_RIGHT_4_PENALTY=0.326376
PRIMER_LEFT_4_SEQUENCE=AGATAGATGCAGAACAGGGGAA
PRIMER_RIGHT_4_SEQUENCE=CTGACCTTTGTTGTTGTCCTTG
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=250,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=59.674
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_01046 Ps_contig_mira-and-tgicl-ass_franssen_9671
SEQUENCE=CYTACTGTTTGCAGTTGCTGCAACTGTTGCTGAAGCTGAAGTTGTTGCACCTGCTGAGGATTTAACCCTGGTGGAACAACACCTTTGGGCTGGTTACTATTCCCACCCTTCTGGTTCTGATTCTGACCTTTGTTGTTGTCCTTGTTTCCACCACCACCTTTACCATTATCAATTTGCATGTTCTTTAACTGATTAACAACATTGTTCTGGTTGTTGTTGTTGTTGTTTGGTTTGGGTACACTCCATAACTCTGCGTGTTTTCCCGATTTTGCAAGCTTCTTGATGAGAATGTTTGGGTCCACATTCCCGGAAACCGTCACCTTCCCCTGTTCTGCAT
TARGET=210,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01047 Ps_contig_mira-and-tgicl-ass_franssen_9673
SEQUENCE=GGTTCGATGACAGAGTTCTTCCCAACAAGAGCAAACGTGCTACCATTATACGGCCCATCAACAAACGAGTAGCTCAGGGACATAAGAAGCCCGGGCTCCTGCTGACACGACGACGCGTAAATACCTTGGGCCCGGCCGACGAGCTTGGACTTCGGGTCAGATGTTTCTGTTAACGGGTCATCCGCCATCCAGATGCTTCCGAACTGTGTAACGAATGGCTTGCTCTTGTCTACCGGTTCAGCTACCTTAACAACGCTTGGGTTTTTTCCGCTGACAGTGTCGTGGAAATAAAACTGAAGGTTTGTTTGTGTTTCTTGGTTCTTTTTTAGAAAACTTTTACCCCAGGTTCCTGCTTTTAGTTTCAGGCTTTGAATCGATGGTGGAAGAATAACACTCAGTATAAGCATCATCGCCACCAGCACATTTTGCTTATACATCTTGGAAGTATTTTTGCAGGGTTGTGTTGTGTTGTGTTATGGATTTTGGTTTCTTCTCTGTGTGTGTGGTGTATATAAATAGTAATTTTTTTTTTCCTTGTGGAGTTAGTCATTTGTGTGGTTTAACTTTTACAACAACATTACTA
TARGET=458,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3909
PRIMER_LEFT_PENALTY=0.055230
PRIMER_RIGHT_PENALTY=0.335679
PRIMER_LEFT_SEQUENCE=CCGGTTCAGCTACCTTAACAAC
PRIMER_RIGHT_SEQUENCE=CCACACACACAGAGAAGAAACC
PRIMER_LEFT=232,22
PRIMER_RIGHT=505,22
PRIMER_LEFT_TM=60.055
PRIMER_RIGHT_TM=59.664
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.4598
PRIMER_LEFT_1_PENALTY=0.124146
PRIMER_RIGHT_1_PENALTY=0.335679
PRIMER_LEFT_1_SEQUENCE=TCAGTATAAGCATCATCGCCAC
PRIMER_RIGHT_1_SEQUENCE=CCACACACACAGAGAAGAAACC
PRIMER_LEFT_1=394,22
PRIMER_RIGHT_1=505,22
PRIMER_LEFT_1_TM=60.124
PRIMER_RIGHT_1_TM=59.664
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=112
PRIMER_PAIR_PENALTY_2=0.5114
PRIMER_LEFT_2_PENALTY=0.175676
PRIMER_RIGHT_2_PENALTY=0.335679
PRIMER_LEFT_2_SEQUENCE=GCTGACAGTGTCGTGGAAATAA
PRIMER_RIGHT_2_SEQUENCE=CCACACACACAGAGAAGAAACC
PRIMER_LEFT_2=269,22
PRIMER_RIGHT_2=505,22
PRIMER_LEFT_2_TM=60.176
PRIMER_RIGHT_2_TM=59.664
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=0.5207
PRIMER_LEFT_3_PENALTY=0.185055
PRIMER_RIGHT_3_PENALTY=0.335679
PRIMER_LEFT_3_SEQUENCE=CTTGTCTACCGGTTCAGCTACC
PRIMER_RIGHT_3_SEQUENCE=CCACACACACAGAGAAGAAACC
PRIMER_LEFT_3=224,22
PRIMER_RIGHT_3=505,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=59.664
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=0.5207
PRIMER_LEFT_4_PENALTY=0.185055
PRIMER_RIGHT_4_PENALTY=0.335679
PRIMER_LEFT_4_SEQUENCE=TGTCTACCGGTTCAGCTACCTT
PRIMER_RIGHT_4_SEQUENCE=CCACACACACAGAGAAGAAACC
PRIMER_LEFT_4=226,22
PRIMER_RIGHT_4=505,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=59.664
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_01048 Ps_contig_mira-and-tgicl-ass_franssen_9673
SEQUENCE=GGTTCGATGACAGAGTTCTTCCCAACAAGAGCAAACGTGCTACCATTATACGGCCCATCAACAAACGAGTAGCTCAGGGACATAAGAAGCCCGGGCTCCTGCTGACACGACGACGCGTAAATACCTTGGGCCCGGCCGACGAGCTTGGACTTCGGGTCAGATGTTTCTGTTAACGGGTCATCCGCCATCCAGATGCTTCCGAACTGTGTAACGAATGGCTTGCTCTTGTCTACCGGTTCAGCTACCTTAACAACGCTTGGGTTTTTTCCGCTGACAGTGTCGTGGAAATAAAACTGAAGGTTTGTTTGTGTTTCTTGGTTCTTTTTTAGAAAACTTTTACCCCAGGTTCCTGCTTTTAGTTTCAGGCTTTGAATCGATGGTGGAAGAATAACACTCAGTATAAGCATCATCGCCACCAGCACATTTTGCTTATACATCTTGGAAGTATTTTTGCAGGGTTGTGTTGTGTTGTGTTATGGATTTTGGTTTCTTCTCTGTGTGTGTGGTGTATATAAATAGTAATTTTTTTTTTCCTTGTGGAGTTAGTCATTTGTGTGGTTTAACTTTTACAACAACATTACTA
TARGET=496,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1452
PRIMER_LEFT_PENALTY=0.088791
PRIMER_RIGHT_PENALTY=1.056374
PRIMER_LEFT_SEQUENCE=GAAAACTTTTACCCCAGGTTCC
PRIMER_RIGHT_SEQUENCE=CCACACAAATGACTAACTCCACA
PRIMER_LEFT=328,22
PRIMER_RIGHT=557,23
PRIMER_LEFT_TM=60.089
PRIMER_RIGHT_TM=59.944
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=1.1805
PRIMER_LEFT_1_PENALTY=0.124146
PRIMER_RIGHT_1_PENALTY=1.056374
PRIMER_LEFT_1_SEQUENCE=TCAGTATAAGCATCATCGCCAC
PRIMER_RIGHT_1_SEQUENCE=CCACACAAATGACTAACTCCACA
PRIMER_LEFT_1=394,22
PRIMER_RIGHT_1=557,23
PRIMER_LEFT_1_TM=60.124
PRIMER_RIGHT_1_TM=59.944
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=1.2321
PRIMER_LEFT_2_PENALTY=0.175676
PRIMER_RIGHT_2_PENALTY=1.056374
PRIMER_LEFT_2_SEQUENCE=GCTGACAGTGTCGTGGAAATAA
PRIMER_RIGHT_2_SEQUENCE=CCACACAAATGACTAACTCCACA
PRIMER_LEFT_2=269,22
PRIMER_RIGHT_2=557,23
PRIMER_LEFT_2_TM=60.176
PRIMER_RIGHT_2_TM=59.944
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=1.3076
PRIMER_LEFT_3_PENALTY=0.251214
PRIMER_RIGHT_3_PENALTY=1.056374
PRIMER_LEFT_3_SEQUENCE=CTCAGTATAAGCATCATCGCCA
PRIMER_RIGHT_3_SEQUENCE=CCACACAAATGACTAACTCCACA
PRIMER_LEFT_3=393,22
PRIMER_RIGHT_3=557,23
PRIMER_LEFT_3_TM=60.251
PRIMER_RIGHT_3_TM=59.944
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=1.4959
PRIMER_LEFT_4_PENALTY=0.439481
PRIMER_RIGHT_4_PENALTY=1.056374
PRIMER_LEFT_4_SEQUENCE=TCCTGCTTTTAGTTTCAGGCTT
PRIMER_RIGHT_4_SEQUENCE=CCACACAAATGACTAACTCCACA
PRIMER_LEFT_4=347,22
PRIMER_RIGHT_4=557,23
PRIMER_LEFT_4_TM=59.561
PRIMER_RIGHT_4_TM=59.944
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_01049 Ps_contig_mira-and-tgicl-ass_franssen_9689
SEQUENCE=AAACATGAACCTCATCATCATCATCCTCTTCAACCTAAATATCACACTTTGCTTCATACTCGTCAATATAGAATTTTGGACAAAGCAGCAATAATTGATGAAGTTGATG
TARGET=12,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01050 Ps_contig_mira-and-tgicl-ass_franssen_9690
SEQUENCE=CTTCAACACCATTCTCCTATCTTCACTCATTTATCTTGTGGGCATGGTTTTCTTGACACTTTCAGTGTCAGTACTAAGACACAAGACACTTTTCTTCTTTGCACTTTATGTGCTTTCCATTGGTGAAGGAGGTCATAAGCCATGTGTTCAGACGTTCGCCGCCGATCAATTCGACGACGATACGCCGGAAGAGAAGGATGCGAAGAGTTCATTTTTCAATTGGTGGTATTTGGGCATTGTCGCCGGTTCAACTGCCGCTGTGTTTATACCTGTTTATCTTCAGGACAATGTTGGGTGGGGAGTGGGGTTAGGAGTATTGGCAGGAGTGTTGGCAATGGCATTGGCAATCTTCTTATTGGGAATCAAAAGGTACAGAAAAGAAGGCCCTACGGGTAGCCCATTCACAAGACTGGCCCAAGTTTTTGTTGCCGCATCTCGCAAGTGGCGCGTGCAAGACACCATTGGTAACTAACTAATTTTTKKTATAGTCAAGAAGACAAACATGAACCTCATCATCATCATCCTCTTACAAACCTAAT
TARGET=510,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01051 Ps_contig_mira-and-tgicl-ass_franssen_9691
SEQUENCE=GAGAGGATGCGAAGAGTTCATTTTTCAATTTTTTGGTTGGTATTTGGGCATTGTCGCCGGTTCAACTGCCGCTGTGTTTATACCTGTTTATCTTCAGGACAATGTTGGGTGGGGAGTGGGGTTAGGAGTATTGGCAGGTAGTTGTTTGGTCAATGGCATTGGCAATCTTCTTATTGGGAATCAAARGGTACAGAAAAGTAGGCCCTACGGGTAGCCCATTCACAAGACTGGCCCAAGTTTTTGTTGCCGCATCTCGCAAGTGGCGCGTGCAAGACACCATTGGTACTAACATTTTTGTTATAGTCAAGAAGACAAACATGAACCTCATCATCATCATCCTCTCAACCTAATATCACTACTTTGCTTA
TARGET=325,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01052 Ps_contig_mira-and-tgicl-ass_franssen_9698
SEQUENCE=TGGAGCCACCAATTTGGACCAGTCCATCAGCAGTGAAGAAGGTAAATCTGTCCTGAGGAACCCATTGAATTTCACTTTCGGAGTCAGACCAAGGAAAGAACTCGAAAGGATCTTCATCGGAGCTGTTGTTGTTGGAATGGTTGGCTTTGGGGGTGAAACGGAGAAGACGAGGTTGAGAGATAGAAGGTGATGAGAGAGGGAAAAAATTGGTATTGAATGTGAAAGGTTTTGAAGGAGAAACGGAATTGAATGGTGGAGAAGAGAATCTGAATCTGAAAAGGGTCGAAGTTTCGGCCATTGAAGACAGAGAGATAGAGAGAGAGAGAAGGAGTGAACCACACGTATTGTCC
TARGET=314,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6735
PRIMER_LEFT_PENALTY=0.054900
PRIMER_RIGHT_PENALTY=0.618623
PRIMER_LEFT_SEQUENCE=GAAGGAGAAACGGAATTGAATG
PRIMER_RIGHT_SEQUENCE=GACAATACGTGTGGTTCACTCC
PRIMER_LEFT=230,22
PRIMER_RIGHT=348,22
PRIMER_LEFT_TM=59.945
PRIMER_RIGHT_TM=59.381
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.6735
PRIMER_LEFT_1_PENALTY=0.054900
PRIMER_RIGHT_1_PENALTY=0.618623
PRIMER_LEFT_1_SEQUENCE=GAAGGAGAAACGGAATTGAATG
PRIMER_RIGHT_1_SEQUENCE=GGACAATACGTGTGGTTCACTC
PRIMER_LEFT_1=230,22
PRIMER_RIGHT_1=349,22
PRIMER_LEFT_1_TM=59.945
PRIMER_RIGHT_1_TM=59.381
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=0.6785
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.618623
PRIMER_LEFT_2_SEQUENCE=GGAATTGAATGGTGGAGAAGAG
PRIMER_RIGHT_2_SEQUENCE=GACAATACGTGTGGTTCACTCC
PRIMER_LEFT_2=241,22
PRIMER_RIGHT_2=348,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=59.381
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.6785
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.618623
PRIMER_LEFT_3_SEQUENCE=GGAATTGAATGGTGGAGAAGAG
PRIMER_RIGHT_3_SEQUENCE=GGACAATACGTGTGGTTCACTC
PRIMER_LEFT_3=241,22
PRIMER_RIGHT_3=349,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=59.381
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=0.8997
PRIMER_LEFT_4_PENALTY=0.281044
PRIMER_RIGHT_4_PENALTY=0.618623
PRIMER_LEFT_4_SEQUENCE=GTCAGACCAAGGAAAGAACTCG
PRIMER_RIGHT_4_SEQUENCE=GACAATACGTGTGGTTCACTCC
PRIMER_LEFT_4=82,22
PRIMER_RIGHT_4=348,22
PRIMER_LEFT_4_TM=60.281
PRIMER_RIGHT_4_TM=59.381
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_01053 Ps_contig_mira-and-tgicl-ass_franssen_9706
SEQUENCE=GTCTTCTTCAATAAGAAACTACTAAATAGAAGTTAAAAAAAAAATTAAAAKRAAGAAACAAAGACCATGATATGTATCATATGATATCAAATCATATACTATATACATCAAATAAAAAAGAAATGAATGATATAAGTATATGATGAAATTTATGTACTTCTTGCTAGCCTCTCATGAAATTCAAGCAATGATTTCTCTTTTTGAAGCCTTATATCTTCACGAAACTTATAGATGAATTCTTCAGCTTTTTGATCCACCTGCATCATTGGAGACTTAGCAACCACAGCTTTCCATGCATCTTCTATAGTATTCATCTCATTGTTCTTGCTGTTGTTGTTATCATCACCATGCACCTTTTCAATCTCTCTACTTGTGTTTCCTTGTGCATGACGAATACTACTACTACTACTGCTCGGTTTGTTGTAGATAGGGATAGGTGCAAAGTTCTTGTGTTGGAAATGGTAATAAGAAGTTGAAGGGGAATATAATGATTTAGAAGAAGTGAGAGAGCGATGATGAGTGGTTTTCGAGGTTATGAGATGAGTGATGGAATGGAAGAGTGAAAGAAGGTGTTGAAGGGTGGTTTTGAAAGCTTTTTGAATGTTGAGTTTGTGGAGTTTTGATTGGAAATTGTTTGAGAGGCTTTTCCATGCTTTTTTGGCTGGTTTGAGTTTCTTTGATAGCTTCAAGCGAGACATGGATGAAAGTGTGTGTGATTGATTCTTGGTTAGAAAAGAGKAGTTTAWGAGAGAAAGAGTTTGATTGAGAAGGGTTGAAAGAATGTCCTAAGGAAGAGTTATTCCTATATTAGGAAAAAGAAAATGATTCTCAATCTCCTCCCCCGCGTACTCTGCGTTAG
TARGET=395,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01054 Ps_contig_mira-and-tgicl-ass_franssen_9713
SEQUENCE=AAAGCCTGAAACTTGATGATGTCCCAGTCAGGATTATACTCAGTCCACTGCTTACCTCTTAGGTTGGCTGGTGCTGAGGGATCACCAATACGATGCATTCTAATCAAGTACTAAGGAGGAAGTGCTGTCTCAAATGCAATGGTAAGGAGGGGATCATCAATGATGTATGTATGTTTAGAGCAAGGAGTGATGACCAAAACGTAGGATCATAGAAAAGTAAGATCCGACCGGTCACCGGTATGTA
TARGET=163,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3636
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.360050
PRIMER_LEFT_SEQUENCE=TGTCTCAAATGCAATGGTAAGG
PRIMER_RIGHT_SEQUENCE=ACCGGTCGGATCTTACTTTTCT
PRIMER_LEFT=125,22
PRIMER_RIGHT=231,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.360
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=0.3785
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.374887
PRIMER_LEFT_1_SEQUENCE=TGTCTCAAATGCAATGGTAAGG
PRIMER_RIGHT_1_SEQUENCE=CCGGTCGGATCTTACTTTTCTA
PRIMER_LEFT_1=125,22
PRIMER_RIGHT_1=230,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=59.625
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=0.4982
PRIMER_LEFT_2_PENALTY=0.138114
PRIMER_RIGHT_2_PENALTY=0.360050
PRIMER_LEFT_2_SEQUENCE=GCTGTCTCAAATGCAATGGTAA
PRIMER_RIGHT_2_SEQUENCE=ACCGGTCGGATCTTACTTTTCT
PRIMER_LEFT_2=123,22
PRIMER_RIGHT_2=231,22
PRIMER_LEFT_2_TM=60.138
PRIMER_RIGHT_2_TM=60.360
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=0.5130
PRIMER_LEFT_3_PENALTY=0.138114
PRIMER_RIGHT_3_PENALTY=0.374887
PRIMER_LEFT_3_SEQUENCE=GCTGTCTCAAATGCAATGGTAA
PRIMER_RIGHT_3_SEQUENCE=CCGGTCGGATCTTACTTTTCTA
PRIMER_LEFT_3=123,22
PRIMER_RIGHT_3=230,22
PRIMER_LEFT_3_TM=60.138
PRIMER_RIGHT_3_TM=59.625
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=108
PRIMER_PAIR_PENALTY_4=0.6296
PRIMER_LEFT_4_PENALTY=0.504940
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=AGCCTGAAACTTGATGATGTCC
PRIMER_RIGHT_4_SEQUENCE=TTGGTCATCACTCCTTGCTCTA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=196,22
PRIMER_LEFT_4_TM=60.505
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_01055 Ps_contig_mira-and-tgicl-ass_franssen_9729
SEQUENCE=TCTTGGAGCAGCACCTTCCATTCCAGCAAGGTCANCTKMAGGAGAWTGTAATCTGGAGACGGGAGGGAGAAGGCATCATCGGCGGCGTGCTTCCGAACAAGATCAACACAAACCACGCTGAAGGACCCTCGTCGAAGCTTCGAGTACGATTGGTTGGTTCCTTTCTCAAGCTTCCTATTTATCCATCTTGATCTCATTTCGTTAGCCTAAAAGCTTTGTTTGTTCTGCCTAACTTTTGTTTGTTTGGTGTGGGTGAGTTTGGATCTGTTGGTTTAAGGTTTTTCTTGGGAAAAATATGGCCGGATCCGTAACCGCCAGGTTGAAATAGGAAGGTTAGTGTTTAGGTTTTGGTTTTTGGGGAAGTGATTCGCCGGAGAAGAAGGTGGCGCCGCCGCCGTTGGCCGGCGACCAACCCCTTCTCCGGTAGGTTGGGAACGGAGAAGATGAAAGGAGTTGCAAAGCAAACCTGGTTATTCTTGAAGAGAGAGAATTATTTTTAACAAAAATCCCCGA
TARGET=235,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01056 Ps_contig_mira-and-tgicl-ass_franssen_9731
SEQUENCE=TTTGMGGGGGGGTAGTACCGGAAGACTGTGGAAGAAGAAGAAGCAGTAATGGAAGACGCAGAGGAAGAGCAACGCCGTCGTTTGAAGCTTGAAGAAGCCCTAGAAATCCAATCCCTCAGACGCATCATTAGCGCCTACCTCAACTATCCTGATGCTGCCGATGAGGATGTTAGAAGATATGAGAGATCTTTTAGGAAGCTTTCACCTGCCCATAAGGATTTGCTATCCCACTATCCTCTAAAGTTTGAAAAATTACGCAGGTGTATATCAATGAACTCTCATTTCATATTCGGCATGCTTCATGCATTTGAACCTCCACTTGATATGAGCCAGGACATAGATTTAAGTGCAGATCAACATCCTGAATATGCCCCAAAAGATCATTTAGTCCATGAAGAGATTAATTCTTGTTCATGTGAATCAGCCCCATTGAGAATAACATGTTCTGTTTCTAATCGACATGGTTGTGTGGAAGGCAGCAATGATTCATGCAGATCATCAGTACTGGTGCATCCCAATGAGGTCACTGACACCTCATCACAGCAACAATGGACGGAGCCATCTTTTCAGTTGAATGTTCCTTTAGTTGACGTTGACAAGGTGCGATGTATTATAAGGAATGTAGTTAGGGACTGGGCTGTAGAGGGTCAAAAGGAACGCGATCAGTGCTACAAGCCTATTCTTGAAGAGCTCAACAGTTTATTTCCTAATCGCAGTAAAGAGAGTCCACCTGCTTGTTTAGTTCCTGGTGCTGGACTCGGTCGGCTGGCTTTGGACATTTCATGTTTAGGATTTATCAGTCAGGGGAATGAGTTTTCATACTACATGATGATATGCTCAAGTTTTATTCTTAACCACTGTGAAACTGCTGGTGAGTGGACAATATATCCTTGGATTCACAGCAATTGCAATTCACTTTCAGATAGTGACCAACTTCGTCCTGTTTCAATACCAGATATTCATCCTGCCAGTGCAGGAATTACCGAAGGTTTTTCCATGTGTGGAGGTGACTTCGTTGAAGTCTACAGTGATGCAAGCCAAATAGGTGCATGGGATGCAGTTGTAACTTGTTTCTTTATTGATACTGCTCACAATATTGTCGAGTACATTGAAATTATTTCACAAATTTTGAAAGATGGAGGAGTTTGGATAAATTTGGGCCCTCTTCTTTATCACTTTGCAGATACGTATGGACAAGACGATGAAATGTCGATTGAATTAAGTTTAGAAGACGTTAAAAGGATTGCATTGAATTATGGATTAGAATTTGAGAAAGAAAGGACCATTGAGACTACCTACACGACCAATCCTAAATCAATGATGCAAAATCGTTACTTTGCTGCATTTTGGACAATGAGAAAAAAATCGGCAGCAACTCATCAGCAAGTTCCCTGAGGTAGGGGGACATGATTGTTGTGATATAAGTTAATAAATTAATTTTCTTTTATCATCTGACTTTGTACCTTTCCTGGATGAAATGGAGAACATTCCCGCATTTGTGAAATCTTGACAATTTGTTTTTATTGGAAAGTTTAACTTGTGACATTTGGTTAAAGAAAAGTATATCATGAATATTTGTTTTKRTAGTGATATGCATGCCAAACAGTATGCATTTTAGATACTATAAGAAAAACGAAAAGTTACGGTGTGGGTTGAAACAAATTATTTGCTTGCAAGTGACACGAGCACTGATTTCATAAACNGCCAA
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01057 Ps_contig_mira-and-tgicl-ass_franssen_9733
SEQUENCE=CATCWGTATTCATCTAGACTCAATATTGTAGGCATGCTAAAGGAAACAATCTAAATACTTTTGTATGGTGGCTCAAGGACACTGGACAGGAGTAAGACGTTGCTGAGTGGAGCACCTCAAAGTTACTCATTCAACCAATCTCTTGAAGAAACCAAAGAGAGAGAGGCTGATGATAATTGGAAAGGAAGTTCGGGTGATAAGCCAAATGACTTCCCACCAGGGGATACAGATGATAGTATTCCAAGTGTTTTATATGACCTCTTGCAGAAAGAAGTTATTGCCTTGAGGAGAGCTGGTCATGAGAAAGACCAAAGCCTAAAAGATAAAGATGATGCCATTGAGATGTTAGCAAAGAAAGTAGATACATTGACCAAAGCTATGGAAGTTGAAGCAAAGAAGATGAGAAGGGAAGTAGCTGCCATGGAAAAGGAGGTAGCTGCTATAGCGTGTCGAGAAAGTACAAGAGAACAGAGCAAAACGGTTCAGCAAA
TARGET=156,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01058 Ps_contig_mira-and-tgicl-ass_franssen_9736
SEQUENCE=CTTCAGTTTTCTGGGCCGATGAATGTGTGCGGGTTACAAATGCTTCCGGGGGCTTGTGATAAACAGAAGATARTGTAGCAATATTGACAAGAAGCTCGTCAAGTAGAGAAGGATCAAGGTTGTTGGAGTCATCAGTTATGACAGGCTTCTCAGCTAACACAACATCCTTAGCTGCCTCTGGATCAGTTGAGAGGAGACGCCAATATATATATGCACGATCTCGCAAATCAGGATTATCTGTCTCCATAGTGGCATTGTTCAAAACAACCTGAATCATCTGCTGTGGACCCTCAGTTGGCTTCTTAAGGAAAAGTTTGACAGTAGCAGTCAGCAATTGTAGTTGGACAAGTGCAGGTTCTTCAGGGAAACTTTCCAAGAAACTTTCAAGAAGCTCGTCAGCATTGTCAATTCTTTCCGCATATTCACCAATTATCCAAATCATTGATGCCTTGGCTTCGGGCTCATCCAAGGTGTCTAAGCTCTCACAAAGAGTTGCAATTATGGACTCATATGTGTTGGGGTATCGTCTAAATATGTCTTTGATAACAATGATTGCCTCTTGAACCACATAATTTACTTTTATCTTGATCAACTCAAGCAATACACTAATGCATCTTTCAGCTGCTCTCTCTAATTTGATGGCACAACGACCAATAGCACGAACAGCCTTTCTAACAAATCTACGTCAACTTCAGTAGCATATTTACCTTTAAAACTTTACCCTAAAACTTAAAAAACCCTTAGGGTTACCTTTAA
TARGET=203,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01059 Ps_contig_mira-and-tgicl-ass_franssen_9743
SEQUENCE=GGGAGTTGTATTTTGTATTATGAAGCGGATACCCTATTATTCCTTTATATTTAAACTGCAAAGAGTAACTGCTTCACWKGGTGTTTGCAATCCCAACTATCACAATCTAGGGTTTTACAATTTTCCCCAAAACCCTAATGGCAGAAGAAACCGCTCCAGAAGTTGTTAATACCAACGCCGCCGTCGTCGATACCAACAACACCGTTGCAACCGCCGTCGATACCGACAACGCCGTTGCTGCTGCTGTCGACATGGAGGTTGAAAACGCTGTTGATTCCAAGGATAAACGACCGAGGGAAGAAAACGAACCCAAAGATGATATCGTTCCGAAGAAGGCTAAAGTTGACGAGGAAAAGTCAGTAGAAGAGCAAAGGTTGGAGAAGTTTGAGAAAACCGAAGATGGAGAAGAAAAGGAAGCATCCGATGCTGTGAAATTGGGACCTAAGACCTTTGGTTCTTCCGTGGAAATGTTTCATTACTTCCACAAATTTCTTAATGCTTGGCCTCATAATTTCTTTGTAAATGTTAACAAGTATGAGCACACGATGTTGCTGGAATTGCTCAAGAATGGGCATGCTGAGTCTGATAAAAAGATTGGCACAGGGGTTTGTGCTTTCCAAATTCGCGAACACCCTCGCTATAAAAATAGGTGCTATTTCCTCATCAGGGACGATGACACAGCTGATGATTTCAGCTTCCGAAAATGTGTGGATCACATAAGTCCCTTGCCAGAAGGGATGCAATTGAAGCCTGAGGGGAACAAGAGGTCTGGTGGTGGTGGAGGAAATCATCATGGAGGAAATGGTGGCAGAGGAAGGGGTGGAAGAGGAGGTGGCCGTGGTGGTCGTGGAAGAGGAGGAAGGGGGAGATATTGATTAATGATAGTCATCGGTGGTCAGGATTTTGTTGAGATCTAAAATGTGTTCTGTACACTTACTTGGCTGGTGTATTTCTTGTTTTCTGCTAATTACTTGATAATTAAGATTTAGGCATTATGGTTATTTATATTTTAAGTAAAATTTTGATATATACCATAACTCTGTATTTTGAATAGGGTTAGGTAAACG
TARGET=770,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01060 Ps_contig_mira-and-tgicl-ass_franssen_9745
SEQUENCE=ATCTCTCTACATTTAAAACTTACATATACGAGATTTTCTTTATTACAGTCAAAAGAAATTTTACTAGCTCAGGATAACAAAGAACCTCTATGCCGAGGAGGCTGCAGCACAGAGAGAGAGGGAACGACAAAGAATGCTTTCTATTCCGGGGCTGATTGCGCCAAGCGAGTTGCAAGACGAAATGGTGGACTCATAGGTTGATTTAGTTGCAAGACGAAATGGTGGACTCATAGGTAGATTAAACAGCGTGGAAGTTAGTATTAATACCATGGGCATTTTAGTGTATTTCACAGGCTCCGTGATTCAATCAATTAACATTGACTCTGAGAATAGGTGCTAACTTTTTTCTCCTAAGTTTAGTCTTTTACTCTT
TARGET=111,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3821
PRIMER_LEFT_PENALTY=0.216588
PRIMER_RIGHT_PENALTY=0.165541
PRIMER_LEFT_SEQUENCE=AACAAAGAACCTCTATGCCGAG
PRIMER_RIGHT_SEQUENCE=CACCATTTCGTCTTGCAACTAA
PRIMER_LEFT=75,22
PRIMER_RIGHT=223,22
PRIMER_LEFT_TM=59.783
PRIMER_RIGHT_TM=60.166
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=0.4581
PRIMER_LEFT_1_PENALTY=0.216588
PRIMER_RIGHT_1_PENALTY=0.241544
PRIMER_LEFT_1_SEQUENCE=AACAAAGAACCTCTATGCCGAG
PRIMER_RIGHT_1_SEQUENCE=AACCTATGAGTCCACCATTTCG
PRIMER_LEFT_1=75,22
PRIMER_RIGHT_1=198,22
PRIMER_LEFT_1_TM=59.783
PRIMER_RIGHT_1_TM=60.242
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=9.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.4596
PRIMER_LEFT_2_PENALTY=0.216588
PRIMER_RIGHT_2_PENALTY=0.243031
PRIMER_LEFT_2_SEQUENCE=AACAAAGAACCTCTATGCCGAG
PRIMER_RIGHT_2_SEQUENCE=ACCTATGAGTCCACCATTTCGT
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=59.783
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.4596
PRIMER_LEFT_3_PENALTY=0.216588
PRIMER_RIGHT_3_PENALTY=0.243031
PRIMER_LEFT_3_SEQUENCE=AACAAAGAACCTCTATGCCGAG
PRIMER_RIGHT_3_SEQUENCE=ACCTATGAGTCCACCATTTCGT
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=234,22
PRIMER_LEFT_3_TM=59.783
PRIMER_RIGHT_3_TM=59.757
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=0.5971
PRIMER_LEFT_4_PENALTY=0.216588
PRIMER_RIGHT_4_PENALTY=0.380537
PRIMER_LEFT_4_SEQUENCE=AACAAAGAACCTCTATGCCGAG
PRIMER_RIGHT_4_SEQUENCE=AATCACGGAGCCTGTGAAATAC
PRIMER_LEFT_4=75,22
PRIMER_RIGHT_4=303,22
PRIMER_LEFT_4_TM=59.783
PRIMER_RIGHT_4_TM=60.381
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_01061 Ps_contig_mira-and-tgicl-ass_franssen_9747
SEQUENCE=CMACACACACACAAAATTGAACATCCTTTTGTAAAGAATTGTATCTTACTACTAATCCTTGCAATGTGCAAACATCTTAGAAAATAACAGAGGCCGGTTACGATAAAACGACCCGGCTTTATTTTCAAAAATCGACTGAACTAATCGGAATGCTAAAGTGGGATACAACTGGGATATCACTGAGCAAAAATGTCATCAATCCCAGAATTGTCCTATACCAATATCCATTCAGGATCATTAGCTACTTGATGATGCTCCTGAGGATTTCTTAGATCTCTTACTCATGCAAATCTTGTTGTAAACAGTCTTGGTTCTAAGTGACAGTAGAATGTCTAAGAAGAATCCAACAACACATGCTCCAGCCATGACTATAAACACCAGTCTATAACAATGCCCTCCGACGCATGTGTTACCGCCTCCTTCTGTTGTCGTTGCCTCCATGTCGTAGAGTATACCGGCAAGTAGCCCGGAGAAGAGGAACGAGCCGAGAGGAAGGTTGAGGATGAGAATATTGTAGATTAGACCGTAGTATTTGAGTCCAAATAACTCGGAAGCTGTTGGGACGGTAACAGCTAAACGCACGCCATAACATATTCCGACGATAATAGAACCGATGTAAAGTGAACCGGGCATAGCCAGGGCTAGGAGTATGTAACCTACAGYCATCAGAATTTGAGATATTGCATTCCAAAGAGGCCTAGGAGTTGCAGATTTCTTGATGAAATGTTCAGAAACCGAACCAGATATGATCCTTCCAAAAAATCCCCAAATGCTAGTCAATGAAACGAATATGGATACATCCGTTATACCCGAGGTGCCGAACCCCGATTTGACCCATTATTATTCACTAACCGACCCAAACCCCGTTTACCAACTCACATAAGAAAACG
TARGET=3,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01062 Ps_contig_mira-and-tgicl-ass_franssen_9752
SEQUENCE=TTCCTCACCTTTTGTAGTACCAATAATTGTAATTCTTTSTRWTCTTTGTCTTTTAGTGTCATGGTTGGGAAGTTCTAGGAAAAGGGTTTGTGCCAAGCCAAGATACTGTCACTGCACCCATGCTTGCTACTGTTACTGGTAGGTAGTGTTTGTCCAAGAGCTTGGTGGTGGGTGATGATTGAATTGTTTGTATTTTGTTCTATCACCCCATTATCTGTGCTTGTTATTTGAGTCTCTTCCAATGTTTCATATCAACCATAGTTTTCTGATGTATTAGTATAGTATTATTTTTAACGTTATAATATAATATAATGTTATATTTCAAAA
TARGET=298,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01063 Ps_contig_mira-and-tgicl-ass_franssen_9756
SEQUENCE=CGTCTTTTTCTACTTCTCCTCATCAAGCGATTGCTGCCAAAGACATTCTTCAGGAGATGCTGTTCGATCATGAATCCAAGTCCGTTCTTCTACACTGAGATGGCCAAGAAAAATACTGTTTGTTAAAAGAGGAATAGGAGCTGATGCAAGTGCTTTTGATCAAAGTAAGCGGTTACGAACAGCAGGGGATTATACACATACCGCTTATGTAACCCCATCTCCTTTTCATCCTCCACCACCACCTGTTTGGGGACCACATGGATATATGGCACCACCACCTCCTCCTCCTTATGATCCATATGCAGGCTATCCTGTTGCACCAGTACCAATGCCTGCTTCAGTTTCTATAGCAGCG
TARGET=278,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0623
PRIMER_LEFT_PENALTY=0.010077
PRIMER_RIGHT_PENALTY=0.052187
PRIMER_LEFT_SEQUENCE=CATACCGCTTATGTAACCCCAT
PRIMER_RIGHT_SEQUENCE=TGGTACTGGTGCAACAGGATAG
PRIMER_LEFT=196,22
PRIMER_RIGHT=327,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.1768
PRIMER_LEFT_1_PENALTY=0.124655
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=TTCTACACTGAGATGGCCAAGA
PRIMER_RIGHT_1_SEQUENCE=TGGTACTGGTGCAACAGGATAG
PRIMER_LEFT_1=87,22
PRIMER_RIGHT_1=327,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.1768
PRIMER_LEFT_2_PENALTY=0.124655
PRIMER_RIGHT_2_PENALTY=0.052187
PRIMER_LEFT_2_SEQUENCE=TCTACACTGAGATGGCCAAGAA
PRIMER_RIGHT_2_SEQUENCE=TGGTACTGGTGCAACAGGATAG
PRIMER_LEFT_2=88,22
PRIMER_RIGHT_2=327,22
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=60.052
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=0.2024
PRIMER_LEFT_3_PENALTY=0.010077
PRIMER_RIGHT_3_PENALTY=0.192310
PRIMER_LEFT_3_SEQUENCE=CATACCGCTTATGTAACCCCAT
PRIMER_RIGHT_3_SEQUENCE=AACTGAAGCAGGCATTGGTACT
PRIMER_LEFT_3=196,22
PRIMER_RIGHT_3=342,22
PRIMER_LEFT_3_TM=59.990
PRIMER_RIGHT_3_TM=60.192
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=5.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=0.2194
PRIMER_LEFT_4_PENALTY=0.167242
PRIMER_RIGHT_4_PENALTY=0.052187
PRIMER_LEFT_4_SEQUENCE=ATCATGAATCCAAGTCCGTTCT
PRIMER_RIGHT_4_SEQUENCE=TGGTACTGGTGCAACAGGATAG
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=327,22
PRIMER_LEFT_4_TM=59.833
PRIMER_RIGHT_4_TM=60.052
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_01064 Ps_contig_mira-and-tgicl-ass_franssen_9757
SEQUENCE=CCTTYCATAAACCTCTCTAGAATAGGGGCATGTATTTTATTATAATTAGTTTCAACTACACAAAAGAGAAACTCACATAAAAAGTTAAATTATAAAGCACTACGTGTAGACAAAGACAAAGATACACAGACGTCAATATCCACACTGTTTCTTCCCCTTTATACGCGTCTTACAATCTTACACGTAATTAGAACATCACACAACAACACAGACCCAGATTCACCCCTTCCAGCAATTCTTTTTTCATTAAACTTCACAACCACTTTTGTTAATATCAATGTTGTCTTTCTCATTCTTTACCTCACTTACCTGCTCCTTTGGTTTCTTCTCTTCAGCCTGTTCATCTTTCTGCTCTTCCTTTTTATCCACAATCTCTATATCAACACTCACTTCAATTTTCTTCTCATCAGAAGCTGGAACCACGGTTTCAATTTCATTGTTGGTAATATCAATAACATCTCCTTTGTGTTCAGGAAGCTTCTCATCATTTACTGGCTTACTGTTTCGGTATATAGCATACAGTGTGATCTGAACTATTCCAAATGTTAACCCCACAACGTTCGGAAGGGTAACATAAATATCTCTGAGAGAAAGACCATACAAGAGCCACATAACCGCGCTTATGAGGAGAAGAAGTGAAAGAGGAAAAGGAAGAAACTCTACACTTTTGGTGCGAATAACAACTCTAATAATACTTAAAGGTGCTGCAAAAACACTAGTGGCAAAAACTACACAGATCCATCCGAGAAGCTTGATACGTCCTTCTTCTTCTTTTGCTAGGACATAAGTTAAGACGACAATCGTGCCGAATCCGACGAAATTCATCAACACAATCATCCTCAATGTCGACATCCTAATTTTCTTGGGACAGTAAGTGACATAAATGGCTAGGTAGATAGTTTCAATCACACAACCAAATGCATTGATGGTGATGAGAAGAGTTTCTCCTGTCTTGATATAAGCATAAAAAAGCCAAAGCATTGCACTGAATAATGCAGCCACATAAGGAATTGATTGAAACCCTTCTGTTGATTTCTTCTTACAAACTCTATAGAATGTTGGTAGTGGTGCCAGAAAGCACACAAAAGATGAAATGTTGCCTAGAACTCCAAAGGCAAAAGATAGATGACTGTGTTGGGACATGGTTATGATCTTATGAAAACTCTTTCTTTCTCTCTCTGAATTGTCCCTTGTGAGAAGGGAGAGAAGGAAGGTTTAAGAGGAAGGTTTAGTTCTAGTTTAGTTTTCTTTGTAACACATTTGATATCCCCGACGA
TARGET=762,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01065 Ps_contig_mira-and-tgicl-ass_franssen_9773
SEQUENCE=TTTTTTGGGTTCGKAGATAGAATAAGTTTCTATTCGCCTTGAGCATAAAGCGGAATTTTCAAGCTGTTTACCTACTTTTTCTTTCTTTCTCACATGGAAAATGTTTCACAATTTTTTTTTTAATTTAATTTAATGTTCATAAATTCTTCAATCCTTCCTCACTAACAACAGCTCTGTCACTCAGCTGTTACTTTTATTTAGGAAAAAAATGGAGTTTCGGGAAACAGAGAAAAAGAAGGTTAACTCAGAGGTTCCATGGATACCCACCACACCTTTTAAGCCCATTGCACAAAGACCAGCGCAGATCTGCACTTCTGGAGAGAGAAATCAGCTGAATTCTCATTTCAAATGGAGAATTTGCATCTCTAGAATCTTCGGCAGGTGGAAATGTTAACCACGAGTCAGAATCTGTGGCGGGAACATCAAACATTGGTCATGACAATGCCAGAACTCGGGTACAGGTGTCGTTCGAAGATGTTCCTGGTCCGAGTAATAGTTTTGCTGAGCTATTGGCTCAAGCAGGAGCTTCCTTCCGCATACTGTAATTCAAGATATGATTTTTTGGTATAACCAAATTTGTTTCCTTGG
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01066 Ps_contig_mira-and-tgicl-ass_franssen_9773
SEQUENCE=TTTTTTGGGTTCGKAGATAGAATAAGTTTCTATTCGCCTTGAGCATAAAGCGGAATTTTCAAGCTGTTTACCTACTTTTTCTTTCTTTCTCACATGGAAAATGTTTCACAATTTTTTTTTTAATTTAATTTAATGTTCATAAATTCTTCAATCCTTCCTCACTAACAACAGCTCTGTCACTCAGCTGTTACTTTTATTTAGGAAAAAAATGGAGTTTCGGGAAACAGAGAAAAAGAAGGTTAACTCAGAGGTTCCATGGATACCCACCACACCTTTTAAGCCCATTGCACAAAGACCAGCGCAGATCTGCACTTCTGGAGAGAGAAATCAGCTGAATTCTCATTTCAAATGGAGAATTTGCATCTCTAGAATCTTCGGCAGGTGGAAATGTTAACCACGAGTCAGAATCTGTGGCGGGAACATCAAACATTGGTCATGACAATGCCAGAACTCGGGTACAGGTGTCGTTCGAAGATGTTCCTGGTCCGAGTAATAGTTTTGCTGAGCTATTGGCTCAAGCAGGAGCTTCCTTCCGCATACTGTAATTCAAGATATGATTTTTTGGTATAACCAAATTTGTTTCCTTGG
TARGET=119,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01067 Ps_contig_mira-and-tgicl-ass_franssen_9774
SEQUENCE=GACCTTTTTTTCTTTTCTCTCCTTCATTTGGTGGTGAAATGATTTTTTTTTTAATTATTTTTCACTAATTTTTATTCAGGTAGACTTGTATAAAGGTTACCAGATCTCATGAGCAGTCGGGGTCATTGTAACAACATCTGCATAAATTTCTCCGAGTTTTGGTTTTTGGTCATATAGAATAAGTTTCTATTCACCTTGAGCATAAAGCGGAATTTTCAAGCTGTTTACCTACTTTTTCTTTCTTTCTCACATGGAAAATGTTTCACAATTTTTTTTTAATTTAATTTAATGTTCATAAATTCTTCAATCCTTCCTCACTAACAACAGCTCTGTCACTCAGCTGTTTACTTTTATTWAGGAAAAAATRGAGTTTCGGGAACAGAAG
TARGET=235,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01068 Ps_contig_mira-and-tgicl-ass_franssen_9774
SEQUENCE=GACCTTTTTTTCTTTTCTCTCCTTCATTTGGTGGTGAAATGATTTTTTTTTTAATTATTTTTCACTAATTTTTATTCAGGTAGACTTGTATAAAGGTTACCAGATCTCATGAGCAGTCGGGGTCATTGTAACAACATCTGCATAAATTTCTCCGAGTTTTGGTTTTTGGTCATATAGAATAAGTTTCTATTCACCTTGAGCATAAAGCGGAATTTTCAAGCTGTTTACCTACTTTTTCTTTCTTTCTCACATGGAAAATGTTTCACAATTTTTTTTTAATTTAATTTAATGTTCATAAATTCTTCAATCCTTCCTCACTAACAACAGCTCTGTCACTCAGCTGTTTACTTTTATTWAGGAAAAAATRGAGTTTCGGGAACAGAAG
TARGET=275,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01069 Ps_contig_mira-and-tgicl-ass_franssen_9784
SEQUENCE=CACCCAAGGCTTGAAAAGAAGGAAAAGTTCTGTTACTTATGTTTTGCATTCATAAATATACAAACCAGCTTTTGTGTTACTTAAAGTAGCAGTTACAATCTCACCAATTTTTTTATTTCCATTACTACCCTTTTCCCTTTTGTCATCATCACCATCATTACCATCACCACCACCATGTTGCTTGTGATTTCCTCCTTGAGCAAGGAGAATCAAACAATTAGCCATGTCTTCTTCATCATCCTCACCATCAGTATTGCTCTCACCACTCGAACAACTCGATGGAGTAGAAACAACACCGCAAGGAGATAAAAGTCTTAACCTCTTTGTGCGTTTTCCTTTGACAACATGAGTTGCATCTTTGTTAGAATAACCAATCAGTTTTTCTTCCATAACAAACTCTTCCATTTTAAATTCTGAAGCAAGAGCAGTTATAATGGGAGAGAGAAGTAACAAAAAAATAAGTGTGTGAGTGAACAAGTTTTGTGTGTGGGTATATATATATATTGGAGAGAGAGTGAGAATAGAGAGAGAAAAAGCGAGAGAGGAGTTTGAAGCCGTCTTATATTTTTG
TARGET=492,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3845
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.347129
PRIMER_LEFT_SEQUENCE=CCTTTGACAACATGAGTTGCAT
PRIMER_RIGHT_SEQUENCE=CAAACTCCTCTCTCGCTTTTTC
PRIMER_LEFT=334,22
PRIMER_RIGHT=550,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.653
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.3845
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.347129
PRIMER_LEFT_1_SEQUENCE=CCTTTGACAACATGAGTTGCAT
PRIMER_RIGHT_1_SEQUENCE=TCAAACTCCTCTCTCGCTTTTT
PRIMER_LEFT_1=334,22
PRIMER_RIGHT_1=551,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.653
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=218
PRIMER_PAIR_PENALTY_2=0.3845
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.347129
PRIMER_LEFT_2_SEQUENCE=CCTTTGACAACATGAGTTGCAT
PRIMER_RIGHT_2_SEQUENCE=TTCAAACTCCTCTCTCGCTTTT
PRIMER_LEFT_2=334,22
PRIMER_RIGHT_2=552,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=59.653
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.5012
PRIMER_LEFT_3_PENALTY=0.154023
PRIMER_RIGHT_3_PENALTY=0.347129
PRIMER_LEFT_3_SEQUENCE=TTCCTTTGACAACATGAGTTGC
PRIMER_RIGHT_3_SEQUENCE=CAAACTCCTCTCTCGCTTTTTC
PRIMER_LEFT_3=332,22
PRIMER_RIGHT_3=550,22
PRIMER_LEFT_3_TM=60.154
PRIMER_RIGHT_3_TM=59.653
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=219
PRIMER_PAIR_PENALTY_4=0.5012
PRIMER_LEFT_4_PENALTY=0.154023
PRIMER_RIGHT_4_PENALTY=0.347129
PRIMER_LEFT_4_SEQUENCE=TTCCTTTGACAACATGAGTTGC
PRIMER_RIGHT_4_SEQUENCE=TCAAACTCCTCTCTCGCTTTTT
PRIMER_LEFT_4=332,22
PRIMER_RIGHT_4=551,22
PRIMER_LEFT_4_TM=60.154
PRIMER_RIGHT_4_TM=59.653
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_01070 Ps_contig_mira-and-tgicl-ass_franssen_9785
SEQUENCE=GACATTGAGAAAGACTACTGATAATTTAGATTCTTCAAGAATACTTGGAGAAGGTGGTTTTGGGATTGTTTTCGGAGGTGTCCTTGGAAATGGGACAAAAGTGGCAGTCAAGGTTCTAAAAAAGAGGGATGATCTTCATAGTAATCATGAATTCTTATCTGAAGTTGAGATGCTTAGTCGTCTTCACCATAGAAATCTGGTCAAGTTGATTGGTATATGTACCGAGGAAGACAGCTTCCGCTGCTTGGTTTATGAACTCGTTACAAATGCAAGCTTGGAATCACATTTACATGGGGTTGACAGAGAAAAGTGTGCACTCGATTGGCGTGCTAGGATGAAGATAGCTCTTGGTGCAGCTCGTGGTTTAGCTTATCTGCATGAAGATTCAAGTCCATCTGTTATTCATAGGGACTTCAAGTCTAGCAACATATTGTTGGAAGATGATTTTACTCCAAAAGTATCTGATTTTGGATTGGCCCGGACCGCCGTGGACGAGGAAAATAGACACATATCAACTCGTGTCGTGGGAACATTCGGCTACGTGGCTCCAGAGTACGCAATGAACGGCCATCTTCTCGTCAAGAGTGATGTATACAGCTATGGCGTCGTTCTTCTAGAGCTTCTGACAGGAAGAAAACCTATAGACATGTCACAAACTCCCGGCCAAGAGAATCTCGTTGCTTGGTCTCGTCAATTTCTCACAAGCAACAAAGGATTAGAAGCAATAATAGATCCATCTATAGGACTCGACGCGCCTTTTGATAGCGTGGCCAAAGTAGCAGCCATTGCTTCAATGTGTGTTGAGCCAGAAGTATCGAACCGTCCATTCATGAGTGAAGTTGTCCAGGCTTTAAAACTTGTATGCAGTGAATGTGAAGAAGAAGAAGCAAAAGAAGATGATGGTTCAAGAAGTTATAGCCAAGAGCTCGAGGATTTATCGGTTGTTGATAATGATATTGAAAATGGTTTGTCAGCATCAGAACTATTCAGTTCTTCTGCTAGAATTGGTAGAATGGAGTATGAAACATTTAGAAGAAAATCATATTCAGGTCCTATGGGAAATGGAAGAAGCAAACAATTGTGGCAGATAATGAGAAGGCTTTCTGGTGGTAGTGTTAGTGATCATGGAACTATATGATCAAGTAATTACTATAGAGAAATAGCGTTCTTTTTTATTTTGTTCTAATTTGTGGATTATTTGTTTTTCTATTGTTATTAATTTAAAAAATAAAACTAAAAAA
TARGET=875,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3122
PRIMER_LEFT_PENALTY=0.108206
PRIMER_RIGHT_PENALTY=0.203971
PRIMER_LEFT_SEQUENCE=AAGAGAATCTCGTTGCTTGGTC
PRIMER_RIGHT_SEQUENCE=ATCAACAACCGATAAATCCTCG
PRIMER_LEFT=665,22
PRIMER_RIGHT=948,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=60.204
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.3415
PRIMER_LEFT_1_PENALTY=0.137489
PRIMER_RIGHT_1_PENALTY=0.203971
PRIMER_LEFT_1_SEQUENCE=GTTGAGCCAGAAGTATCGAACC
PRIMER_RIGHT_1_SEQUENCE=ATCAACAACCGATAAATCCTCG
PRIMER_LEFT_1=799,22
PRIMER_RIGHT_1=948,22
PRIMER_LEFT_1_TM=60.137
PRIMER_RIGHT_1_TM=60.204
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=150
PRIMER_PAIR_PENALTY_2=0.3525
PRIMER_LEFT_2_PENALTY=0.148509
PRIMER_RIGHT_2_PENALTY=0.203971
PRIMER_LEFT_2_SEQUENCE=TTCATGAGTGAAGTTGTCCAGG
PRIMER_RIGHT_2_SEQUENCE=ATCAACAACCGATAAATCCTCG
PRIMER_LEFT_2=826,22
PRIMER_RIGHT_2=948,22
PRIMER_LEFT_2_TM=60.149
PRIMER_RIGHT_2_TM=60.204
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.3725
PRIMER_LEFT_3_PENALTY=0.148509
PRIMER_RIGHT_3_PENALTY=0.224014
PRIMER_LEFT_3_SEQUENCE=TTCATGAGTGAAGTTGTCCAGG
PRIMER_RIGHT_3_SEQUENCE=AAAGCCTTCTCATTATCTGCCA
PRIMER_LEFT_3=826,22
PRIMER_RIGHT_3=1102,22
PRIMER_LEFT_3_TM=60.149
PRIMER_RIGHT_3_TM=60.224
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.3873
PRIMER_LEFT_4_PENALTY=0.183296
PRIMER_RIGHT_4_PENALTY=0.203971
PRIMER_LEFT_4_SEQUENCE=ATTGCTTCAATGTGTGTTGAGC
PRIMER_RIGHT_4_SEQUENCE=ATCAACAACCGATAAATCCTCG
PRIMER_LEFT_4=784,22
PRIMER_RIGHT_4=948,22
PRIMER_LEFT_4_TM=60.183
PRIMER_RIGHT_4_TM=60.204
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_01071 Ps_contig_mira-and-tgicl-ass_franssen_9787
SEQUENCE=TATTTACATCAATCCGGTGGCAAGCCTAGGGAGCAAATCAATTGCATCAGCAATGTTCTGCNGTTGATTTTCGACATAGCCTGCATCTTTGTTATTCACATTACTATTTGAATCAATCAATCGTTCGGCAACCAACGAAAGCAACTTCTCCTGTGAGACTTCTGCGACATCCAGGCTCAAACCTAACTGGTTTCTTAGCAGAAGAACATTACAAATAGCGAGGAGAGGACAAGGTCCATTGTCGTTTTGAAGGACGATCGGAGTAGTGCGTCCAAGAAATTGAATCGCTTTGGTTTTGTGAAGAAAATCTTTAACTGGAGAAAGCTGTTGTTGTTCTTGTTGAAGTTGATGTTGAAGATCGTGATGTTGATTTTGATTCTGAGGGTCTTCCGATGGGGAAGAAGACGGTGGTGCCGCCGTGTCCATGGCGATGTGTGTATGTGCGTTATTGACAGAGGTSAGAAATTAAAGGTGGTGTTAGAAATTAATAAGACG
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01072 Ps_contig_mira-and-tgicl-ass_franssen_9788
SEQUENCE=CAGTTGTTGCGGCCACAAAATCAACACAATCTTCTTCTTCTGGGTTTATTTTTTGGTAGGGTTTCGACATTCCGTGTTTCAAGAGAAACAAGCTCTCCCATAAGGGCATTATAGGACTTCGATCCAATTGCAGTTGCAGTATCATGATCCTGCGGATCAACTATCCAGCCATGATACAATGGGATGTCCAGCAAATCGAATATGGCACACTCTGAAGTGAATTCAAAATCATCTATTCTACGTTAAATTTTAGATTTACATCAATTCCGGTGGCAAGCCTAGGGAGCAAATCAATTG
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.9733
PRIMER_LEFT_PENALTY=3.674441
PRIMER_RIGHT_PENALTY=0.298898
PRIMER_LEFT_SEQUENCE=GGCCACAAAATCAACACAAT
PRIMER_RIGHT_SEQUENCE=TGAATTCACTTCAGAGTGTGCC
PRIMER_LEFT=10,20
PRIMER_RIGHT=224,22
PRIMER_LEFT_TM=58.326
PRIMER_RIGHT_TM=60.299
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=215
PRIMER_PAIR_PENALTY_1=3.9733
PRIMER_LEFT_1_PENALTY=3.674441
PRIMER_RIGHT_1_PENALTY=0.298898
PRIMER_LEFT_1_SEQUENCE=GGCCACAAAATCAACACAAT
PRIMER_RIGHT_1_SEQUENCE=GAATTCACTTCAGAGTGTGCCA
PRIMER_LEFT_1=10,20
PRIMER_RIGHT_1=223,22
PRIMER_LEFT_1_TM=58.326
PRIMER_RIGHT_1_TM=60.299
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=3.9743
PRIMER_LEFT_2_PENALTY=3.674441
PRIMER_RIGHT_2_PENALTY=0.299855
PRIMER_LEFT_2_SEQUENCE=GGCCACAAAATCAACACAAT
PRIMER_RIGHT_2_SEQUENCE=TGGATCGAAGTCCTATAATGCC
PRIMER_LEFT_2=10,20
PRIMER_RIGHT_2=125,22
PRIMER_LEFT_2_TM=58.326
PRIMER_RIGHT_2_TM=60.300
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=4.0773
PRIMER_LEFT_3_PENALTY=3.674441
PRIMER_RIGHT_3_PENALTY=0.402858
PRIMER_LEFT_3_SEQUENCE=GGCCACAAAATCAACACAAT
PRIMER_RIGHT_3_SEQUENCE=TTCAGAGTGTGCCATATTCGAT
PRIMER_LEFT_3=10,20
PRIMER_RIGHT_3=215,22
PRIMER_LEFT_3_TM=58.326
PRIMER_RIGHT_3_TM=59.597
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=4.1175
PRIMER_LEFT_4_PENALTY=3.674441
PRIMER_RIGHT_4_PENALTY=0.443036
PRIMER_LEFT_4_SEQUENCE=GGCCACAAAATCAACACAAT
PRIMER_RIGHT_4_SEQUENCE=AATTGATTTGCTCCCTAGGCTT
PRIMER_LEFT_4=10,20
PRIMER_RIGHT_4=295,22
PRIMER_LEFT_4_TM=58.326
PRIMER_RIGHT_4_TM=60.443
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_01073 Ps_contig_mira-and-tgicl-ass_franssen_9793
SEQUENCE=ATTATTGGTACGAATACATACAGCATAACATAATATACATTTGTAAACTTCTAATGAAATACAGATATTAAAGAAGCTATTCAAGTTGTGAAACTAAGGGCTRTATATATATATATATCACTGATCACTAATTGCAGATGCATCAAATCAAAGATCTACACTTACATGGATAGAAGTTACATGCCCCAGATGCCACGAGCTGTTGCCACTTTGTTACTTGGTCGAGATGTTAGTTCATACAATATCCTCGCAAACGTGTCAGAATATTTGCTATCTGTGAAAAGTATCTCAGAAAAAGCATTCTCATAGGCTAGCAACAAAATTCTGTTTTGTGGCTCAGAAACAAG
TARGET=104,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01074 Ps_contig_mira-and-tgicl-ass_franssen_9806
SEQUENCE=GGGTTTTGTGGTTTGTGGCTTTCCTTCACAGAGGATGCATTCGAAAGGAGAACAAGGGAACCAGACAAGGTCACTGCCTGTGTCCATGTAGAGAGTTATGAGTTGTGGAGGGTGTGAACCTAGGTTGAAAGAAAGTGTGTAATCACTACCGGGAGAGAGTGGGAGAGAAACTTGGGGTTGGTGGTGGTTGTTGTTGTGTTGGTGGTGGAAGCGTTTTATGGAGCGGGTTGAGGTCGATTTGAGGAGGTGGTGGGTACTGTTGAATTGGGTATTGGAAATTGAGTGTGTTAAGGGTAATAGAATAATTTGAGACGAGGGTGAAAAACAGAGTATGAAACATAGTAGTAGTAGAAGAAGCAACATGGGAGAAGACATTGATTGTTTGTTATTAAGAGGAAGAGAGAACAGAAGAGAGAGTAATAATGAGATAGAGAGAGAGAGAGGGGATTTGAATTGAATTGAGGATTACCCCCGCGTACT
TARGET=340,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1575
PRIMER_LEFT_PENALTY=0.153161
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=GAGGTGGTGGGTACTGTTGAAT
PRIMER_RIGHT_SEQUENCE=AATGTCTTCTCCCATGTTGCTT
PRIMER_LEFT=243,22
PRIMER_RIGHT=375,22
PRIMER_LEFT_TM=60.153
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=0.2801
PRIMER_LEFT_1_PENALTY=0.275749
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=TGGGTATTGGAAATTGAGTGTG
PRIMER_RIGHT_1_SEQUENCE=AATGTCTTCTCCCATGTTGCTT
PRIMER_LEFT_1=265,22
PRIMER_RIGHT_1=375,22
PRIMER_LEFT_1_TM=59.724
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=0.4143
PRIMER_LEFT_2_PENALTY=0.409918
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=TATGAGTTGTGGAGGGTGTGAA
PRIMER_RIGHT_2_SEQUENCE=AATGTCTTCTCCCATGTTGCTT
PRIMER_LEFT_2=96,22
PRIMER_RIGHT_2=375,22
PRIMER_LEFT_2_TM=60.410
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.4143
PRIMER_LEFT_3_PENALTY=0.409918
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=TTATGAGTTGTGGAGGGTGTGA
PRIMER_RIGHT_3_SEQUENCE=AATGTCTTCTCCCATGTTGCTT
PRIMER_LEFT_3=95,22
PRIMER_RIGHT_3=375,22
PRIMER_LEFT_3_TM=60.410
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.6276
PRIMER_LEFT_4_PENALTY=0.623261
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=GTTATGAGTTGTGGAGGGTGTG
PRIMER_RIGHT_4_SEQUENCE=AATGTCTTCTCCCATGTTGCTT
PRIMER_LEFT_4=94,22
PRIMER_RIGHT_4=375,22
PRIMER_LEFT_4_TM=59.377
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_01075 Ps_contig_mira-and-tgicl-ass_franssen_9806
SEQUENCE=GGGTTTTGTGGTTTGTGGCTTTCCTTCACAGAGGATGCATTCGAAAGGAGAACAAGGGAACCAGACAAGGTCACTGCCTGTGTCCATGTAGAGAGTTATGAGTTGTGGAGGGTGTGAACCTAGGTTGAAAGAAAGTGTGTAATCACTACCGGGAGAGAGTGGGAGAGAAACTTGGGGTTGGTGGTGGTTGTTGTTGTGTTGGTGGTGGAAGCGTTTTATGGAGCGGGTTGAGGTCGATTTGAGGAGGTGGTGGGTACTGTTGAATTGGGTATTGGAAATTGAGTGTGTTAAGGGTAATAGAATAATTTGAGACGAGGGTGAAAAACAGAGTATGAAACATAGTAGTAGTAGAAGAAGCAACATGGGAGAAGACATTGATTGTTTGTTATTAAGAGGAAGAGAGAACAGAAGAGAGAGTAATAATGAGATAGAGAGAGAGAGAGGGGATTTGAATTGAATTGAGGATTACCCCCGCGTACT
TARGET=430,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.3148
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=3.310420
PRIMER_LEFT_SEQUENCE=AAGCAACATGGGAGAAGACATT
PRIMER_RIGHT_SEQUENCE=GGGGGTAATCCTCAATTCAA
PRIMER_LEFT=354,22
PRIMER_RIGHT=472,20
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=58.690
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=3.4249
PRIMER_LEFT_1_PENALTY=0.114470
PRIMER_RIGHT_1_PENALTY=3.310420
PRIMER_LEFT_1_SEQUENCE=AGAAGAAGCAACATGGGAGAAG
PRIMER_RIGHT_1_SEQUENCE=GGGGGTAATCCTCAATTCAA
PRIMER_LEFT_1=349,22
PRIMER_RIGHT_1=472,20
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=58.690
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=3.5535
PRIMER_LEFT_2_PENALTY=0.243031
PRIMER_RIGHT_2_PENALTY=3.310420
PRIMER_LEFT_2_SEQUENCE=AAGAAGCAACATGGGAGAAGAC
PRIMER_RIGHT_2_SEQUENCE=GGGGGTAATCCTCAATTCAA
PRIMER_LEFT_2=351,22
PRIMER_RIGHT_2=472,20
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=58.690
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=3.5915
PRIMER_LEFT_3_PENALTY=0.281044
PRIMER_RIGHT_3_PENALTY=3.310420
PRIMER_LEFT_3_SEQUENCE=GAGACGAGGGTGAAAAACAGAG
PRIMER_RIGHT_3_SEQUENCE=GGGGGTAATCCTCAATTCAA
PRIMER_LEFT_3=308,22
PRIMER_RIGHT_3=472,20
PRIMER_LEFT_3_TM=60.281
PRIMER_RIGHT_3_TM=58.690
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=3.8062
PRIMER_LEFT_4_PENALTY=0.495821
PRIMER_RIGHT_4_PENALTY=3.310420
PRIMER_LEFT_4_SEQUENCE=ATTTGAGACGAGGGTGAAAAAC
PRIMER_RIGHT_4_SEQUENCE=GGGGGTAATCCTCAATTCAA
PRIMER_LEFT_4=304,22
PRIMER_RIGHT_4=472,20
PRIMER_LEFT_4_TM=59.504
PRIMER_RIGHT_4_TM=58.690
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=169
=
PRIMER_SEQUENCE_ID=SSR_01076 Ps_contig_mira-and-tgicl-ass_franssen_9814
SEQUENCE=TAAAATACAACCAACNCAAATATTYCAGTTTTTATGAGACGAGAAAAATCCTAGAAATTTTACTCATAGAAAAATTATGGGTTATGACATCAACCCATCTTATGATTCCAAATCAACATAATTCTTGATCTAAAATTCAAACAGAAACTAAAATAACAAACAAACAGCTTAGACATTTCTCCTTAAACTGATATCTATTTGTTTGAAGCAACATGCCTTTCATAGTGACCTTTGTAATAGGCCATAAGGGTTTCCCTAGGAGGCAATGTTTCCTTAACAATTGGGTGATTGTTGAAGTCTTGGCTCCAATTGTAAAGCTTGGGAAATTTGTCACTAGTAAACAACTTCGCTCCAAATGCTTCTTGTATAATAGGGAGCCAGTAAGCTATAAGAACAGCAGTAATGTCAACAATTCCAATCTCATCTCCACCAAAGAATTTACCCTTGAGCTCATTCTCAAAAAGATGAAGAGCCTCTTCTATTTCCTCAACACCTTTCTCACGTTCTTTCTCATCATGAGTGTATAGAGCTTTCCGTGCAGCATTAATAATCTTGTCATCAATGAATTTTGACCAGAAACGAGCCAAGGCTCTTTTGTAAGGATCAGAAGGCAAGATTGGATTTTGCTTCCATGTTTCATCGATGTACTCAAGAATGACAAGTGATTCTGAGATTGGTTTTCCATTATGAACTAATACAGGAACTTTTTTATAAACTGGATTATATTTGAGGAGAAACTCACTCTTGTTTTGCAAGTTTTCTACTTCATATTTGTATTCAACTCCCTTCAATTTTAGAGCAATATCGACCCTACTCACAAATGGACTTTCCACCATTCCTAAAAGCTTCACATCTTCCTTATTTGATGCCATCTTAGTGCTTCAAATCTTTACTCAAACAACTGTTTTAATCTCTGATCCTSAYTTCTAGTACCTACTTCTATATATAGAGAGAGCCAAGATTATTATTCAACAAAGACGGTC
TARGET=155,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01077 Ps_contig_mira-and-tgicl-ass_franssen_9842
SEQUENCE=TTTGGAACAAAACCCTTACCAGACCCCTTCGACTTCGAAATTGTAACCCTTTCYTTTTGTTTGTCTTTTCCTAGAAAAATCTGAACTAGCATTAACACAAGAAACTCCTCTACTTGATTTACCCCATGAAGAAGAAGTTAAACCAGAAAATGGAGCGATGTTCAAATTCAAACAACCGCCTCTATTAGGGAACGCCATTCTACATCTAAAATGTAACGAAATCTCCATCTTAATTAGTTACTAACTTTGTTACGAAAGACATTTGTATTGATGAATAATGATAAATTAAAAACAAACACAAGGTTWWTGTGAATCCGTGACGACGTTGAGTTTTTGTGGTCGGTTGGTTGGTTGT
TARGET=342,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01078 Ps_contig_mira-and-tgicl-ass_franssen_9846
SEQUENCE=GCTTGTTCTCCAAGTAACACTCGCATGCCAAATTCTTCTTTGCCACTCTCTTTAAACCATATCTCAAACCTCACCTAACTCACATTTTCTCACACCTCTCTCTCTCTTACACACACAGTCACAGTAAGAACTATCAACTATGGATGGTTCTCCTGCAAACCCTAACCCTTCACACAAACCAAAACAACCGCTTCGACCCTGTGACTATTGCAGCAATTCAACTGCAGTTCTATACTGCAGAGCCGATTCAGCCAAACTCTGTTTCTCTTGCGACCGTGAAGTCCACTCTACAAACCAGCTTTTCTCAAAGCACACGCGCTCCTTAATCTGTGATTCATGCGATGATTCCCCTGCTACTATACTCTGTTCGACGGAATCCTCTGTTTTCTGTCAGAATTGTGATTGGGAGAATCATAATCTCTCGCTTTGTTCTCCGCATGAAAGAAGACCTCTTGAGGGTTTCACTGGGTGTCCTTCGGTTACTGAACTGTTGGCGATTGTTGGGTTGCAGGATATTGGGAAAAAATCGTTGCTTTTTCCTGAAGAAAGTTTTGGGGATGGGTTTCTAGGGTATGAGATTGAAGGGGTGTCTGATATGTTTGTTTGGGATGCTCCTACTTTTGTTAGTCTTGATGATCTTGTTTCTTCTTCTCCTTCTTCTCATAACTATAGTGCTATGGAGGTTCCTCCTTTGCCCAAGAATCGTAAGGCCGCATGTGGGAGACAGAAAGAAGAGATTTTAAGTCAGCTACGCGAGATGACGAAGTCTGAGCCCTTGGATCAGGAAGATTATATGCCATCAAGGAGTTTGTCTATAGATTTTGAACACGACACTAAGGCTGATATTGTTCCTATAAATGAGTGGCTCAGAGAAAGCAGCGAACCAATTTATCAAGTTGTGCCTGTTGATACATCAATCAGAGCTCATACCGAAGAATTTCCAGTTAAACATTCTGTATCTTCTGTCGGGGAGTCTCATACTCACATGACACCTTCAGAATCTATTAAGTCTGAAACCTTGTCGACTACTTTTAAACCTCTTCCTCCTCCATATGAATTAGCGAGTCAAGAAAGGGATTCTGCATTGTTACGGTACAAGCAGAAGAAGAAAACAAGAAGATACGACAAGCATATAAGGTATGAATCACGAAAAGTTCGGGCGGAAACCAGAACACGGGTTAAGGGCCGATTTGCTAAGATAGAGCATTGAAATCACGGTAGGCTGCCTGCTACAACAACGTAGCTAAAATTCTTGGCTCGTGTCGCATAAATCATGTAACATGTCTCTTGTATAGTGAACTTATTTAAATTTTGCATGTATGTAAGGTAAGATGTATCGATCCCTGGATCCCGAGGTTTTGAAGTTATGCAATATCAAAGTAGTAGTATTGCTATCAGATTTTGTCTGATATAGAAAAACACAGTATCAGTTTTGTTCTTTCTGTATTTGAATTTGAATTACCCTTGGATTGGTGTTGGAAGAGCTTGAATAATAAGTGCTTTGGAGCATATTACCTAAACC
TARGET=96,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2409
PRIMER_LEFT_PENALTY=0.237699
PRIMER_RIGHT_PENALTY=0.003166
PRIMER_LEFT_SEQUENCE=CCAAATTCTTCTTTGCCACTCT
PRIMER_RIGHT_SEQUENCE=TGAATCGGCTCTGCAGTATAGA
PRIMER_LEFT=27,22
PRIMER_RIGHT=249,22
PRIMER_LEFT_TM=59.762
PRIMER_RIGHT_TM=60.003
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=223
PRIMER_PAIR_PENALTY_1=0.3041
PRIMER_LEFT_1_PENALTY=0.237699
PRIMER_RIGHT_1_PENALTY=0.066394
PRIMER_LEFT_1_SEQUENCE=CCAAATTCTTCTTTGCCACTCT
PRIMER_RIGHT_1_SEQUENCE=GCTTTGAGAAAAGCTGGTTTGT
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=310,22
PRIMER_LEFT_1_TM=59.762
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=0.4023
PRIMER_LEFT_2_PENALTY=0.237699
PRIMER_RIGHT_2_PENALTY=0.164560
PRIMER_LEFT_2_SEQUENCE=CCAAATTCTTCTTTGCCACTCT
PRIMER_RIGHT_2_SEQUENCE=GTTTTGGTTTGTGTGAAGGGTT
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=184,22
PRIMER_LEFT_2_TM=59.762
PRIMER_RIGHT_2_TM=60.165
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=158
PRIMER_PAIR_PENALTY_3=0.4599
PRIMER_LEFT_3_PENALTY=0.237699
PRIMER_RIGHT_3_PENALTY=0.222249
PRIMER_LEFT_3_SEQUENCE=CCAAATTCTTCTTTGCCACTCT
PRIMER_RIGHT_3_SEQUENCE=TTTGTGTGAAGGGTTAGGGTTT
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=177,22
PRIMER_LEFT_3_TM=59.762
PRIMER_RIGHT_3_TM=59.778
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.5787
PRIMER_LEFT_4_PENALTY=0.575571
PRIMER_RIGHT_4_PENALTY=0.003166
PRIMER_LEFT_4_SEQUENCE=TTGTTCTCCAAGTAACACTCGC
PRIMER_RIGHT_4_SEQUENCE=TGAATCGGCTCTGCAGTATAGA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=249,22
PRIMER_LEFT_4_TM=59.424
PRIMER_RIGHT_4_TM=60.003
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_01079 Ps_contig_mira-and-tgicl-ass_franssen_9863
SEQUENCE=CGAAATCGGAACACAGTAGGTGGGTGATATGTCGAGTTTACGAGCGCGATGAAGACGATGATGAAGATGGAGATRGAACGGAGTTATCTTGTTYGGATGAAGTTTTCTTATCATTGGATGATCTTGATGAAGTAAGCTTGCCGAATTAGAAGATGCAAATGCAATGCAAGTGATAATAATAATAATACTATAGAGAGAAAGAGAGTTTAATTAGTAGCTACATTTTCCAAATGGGAGATAATTAAAGGTTTAAGCTAATTGTTGTTGTTATGAA
TARGET=173,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01080 Ps_contig_mira-and-tgicl-ass_franssen_9866
SEQUENCE=TTTAGTTATGAATTATGAATTTGAACTCTTATGATGTTTTAGTTGTTTATTATTTATTTATCTGTGTGACTCTTTTTGTGAATAAAAAAATCAATAAAAAATGAATTGTAAATTT
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01081 Ps_contig_mira-and-tgicl-ass_franssen_9880
SEQUENCE=AATTTCATCCTCGAATCCCAATAGTTCAGCAATTTCATCAACAGTGGTTTTCCCATTGGAAAACCTACCAGAAGGTCCATCAGGGAAATCAATTCCGTAAGGAAGATAATCAGCTCTAGCCAAAGATCTAAGAACATTGTTGTTACCATTATCCACCAAAGAATCACCAAAAATGAAGTAACAAGGAACTTGTGGTGCAGCTCTAACTCCACTCGAGAAACAAAATATGACAACGGACAACAATGCAAGCATGGTTATGAGATCCTGAAATGCCATGGAAGAAAGAAATGGTAGGTAGAAGATAGATAGTGAGAATGAATGAATGAATCCCCGMGTACTCTGCGTTG
TARGET=313,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01082 Ps_contig_mira-and-tgicl-ass_franssen_9885
SEQUENCE=TTTAAGTTTAACTAACAATACATCNTACACANGTATTTAAGGTAAACAACCTAAAATACTTAAAAAATTACCCCTTTGGAACCCCGTTTAACACCACTTGTTTAATTCATCATGGCTCAAATTTGTTAGCAGGCTTTCTCTGATTGCATAAACCTTCAAATGCTTGATTTTGTAGCATTAACATTGGATTTTGAAGATACTCATTCCACTTAGTTTCTTCTTCTTCTGTTTGATTCTCTTTAATAGAGGTCTCACAATCTGTCAATCCCCAAGGAGATAGCATATTATTCTTCATATGAAAAGAATCCGAAGAAACCGAAACAT
TARGET=215,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1203
PRIMER_LEFT_PENALTY=0.075444
PRIMER_RIGHT_PENALTY=0.044904
PRIMER_LEFT_SEQUENCE=GCTCAAATTTGTTAGCAGGCTT
PRIMER_RIGHT_SEQUENCE=CTATCTCCTTGGGGATTGACAG
PRIMER_LEFT=114,22
PRIMER_RIGHT=279,22
PRIMER_LEFT_TM=59.925
PRIMER_RIGHT_TM=59.955
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.2656
PRIMER_LEFT_1_PENALTY=0.220659
PRIMER_RIGHT_1_PENALTY=0.044904
PRIMER_LEFT_1_SEQUENCE=CATGGCTCAAATTTGTTAGCAG
PRIMER_RIGHT_1_SEQUENCE=CTATCTCCTTGGGGATTGACAG
PRIMER_LEFT_1=110,22
PRIMER_RIGHT_1=279,22
PRIMER_LEFT_1_TM=59.779
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.5343
PRIMER_LEFT_2_PENALTY=0.489375
PRIMER_RIGHT_2_PENALTY=0.044904
PRIMER_LEFT_2_SEQUENCE=ATGGCTCAAATTTGTTAGCAGG
PRIMER_RIGHT_2_SEQUENCE=CTATCTCCTTGGGGATTGACAG
PRIMER_LEFT_2=111,22
PRIMER_RIGHT_2=279,22
PRIMER_LEFT_2_TM=60.489
PRIMER_RIGHT_2_TM=59.955
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.5486
PRIMER_LEFT_3_PENALTY=0.503688
PRIMER_RIGHT_3_PENALTY=0.044904
PRIMER_LEFT_3_SEQUENCE=CAGGCTTTCTCTGATTGCATAA
PRIMER_RIGHT_3_SEQUENCE=CTATCTCCTTGGGGATTGACAG
PRIMER_LEFT_3=129,22
PRIMER_RIGHT_3=279,22
PRIMER_LEFT_3_TM=59.496
PRIMER_RIGHT_3_TM=59.955
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.7552
PRIMER_LEFT_4_PENALTY=0.710297
PRIMER_RIGHT_4_PENALTY=0.044904
PRIMER_LEFT_4_SEQUENCE=TTTGTTAGCAGGCTTTCTCTGA
PRIMER_RIGHT_4_SEQUENCE=CTATCTCCTTGGGGATTGACAG
PRIMER_LEFT_4=121,22
PRIMER_RIGHT_4=279,22
PRIMER_LEFT_4_TM=59.290
PRIMER_RIGHT_4_TM=59.955
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_01083 Ps_contig_mira-and-tgicl-ass_franssen_9896
SEQUENCE=CTCTCCTCAAAGCATCAACTTCCTTCAACAAATCAAATTCCAAACCTTTCTTCTTCAACTCCTCCGAACACTTCCTCAACAACCCTCTATCCTCTTTCTCCCTACAAAGCATCTTCTCCATATGACCCTCCACGTCACTCTTATACCTTAAAGCCCATATAAACCCCAACGAAAACAACAAAGAACTTAGCGACGGAACCCACGATTTCTGACAGGCTTTGCTAGGTGTTGTTGTTGTTGAAGATGATGAAGAGTTGAAGAGAAGCATGAGAGTGGTTGAATGGAAGAGGAAGAAGAAGATGTAGAGCATTGTGATCTCTTTTCGGATTGAATCTATTTGGGTTTCTTTTAAAGCTATGCGTCCGTTGATGAGTTCTTCTTCTTTGAGCCATTGCTTTAATAGGAGTTTGTGGTTGGTGTTCGGCTATTTG
TARGET=227,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0801
PRIMER_LEFT_PENALTY=0.058944
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=CCTTAAAGCCCATATAAACCCC
PRIMER_RIGHT_SEQUENCE=AAGCAATGGCTCAAAGAAGAAG
PRIMER_LEFT=145,22
PRIMER_RIGHT=396,22
PRIMER_LEFT_TM=59.941
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.1082
PRIMER_LEFT_1_PENALTY=0.087031
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=ATTTCTGACAGGCTTTGCTAGG
PRIMER_RIGHT_1_SEQUENCE=AAGCAATGGCTCAAAGAAGAAG
PRIMER_LEFT_1=204,22
PRIMER_RIGHT_1=396,22
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.1191
PRIMER_LEFT_2_PENALTY=0.008930
PRIMER_RIGHT_2_PENALTY=0.110131
PRIMER_LEFT_2_SEQUENCE=CTTTCTTCTTCAACTCCTCCGA
PRIMER_RIGHT_2_SEQUENCE=TCCTCTTCCATTCAACCACTCT
PRIMER_LEFT_2=45,22
PRIMER_RIGHT_2=290,22
PRIMER_LEFT_2_TM=59.991
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=0.1191
PRIMER_LEFT_3_PENALTY=0.008930
PRIMER_RIGHT_3_PENALTY=0.110131
PRIMER_LEFT_3_SEQUENCE=CTTTCTTCTTCAACTCCTCCGA
PRIMER_RIGHT_3_SEQUENCE=CCTCTTCCATTCAACCACTCTC
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=289,22
PRIMER_LEFT_3_TM=59.991
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.1264
PRIMER_LEFT_4_PENALTY=0.016312
PRIMER_RIGHT_4_PENALTY=0.110131
PRIMER_LEFT_4_SEQUENCE=GAACACTTCCTCAACAACCCTC
PRIMER_RIGHT_4_SEQUENCE=CCTCTTCCATTCAACCACTCTC
PRIMER_LEFT_4=65,22
PRIMER_RIGHT_4=289,22
PRIMER_LEFT_4_TM=60.016
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_01084 Ps_contig_mira-and-tgicl-ass_franssen_9897
SEQUENCE=ACAACTGTCTCTACATCTTCTTCTTCCTTCTTCCTATTCAACCACTCTCATGCTTCTCTTCAACTCTTCATACATCTTCCAAACAACAACAACACCTAGA
TARGET=82,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01085 Ps_contig_mira-and-tgicl-ass_franssen_9898
SEQUENCE=AACTTTTGGTTTTGTTTTTTCTTTCTTTCTGGATTCTTCTTCGCCATGTCTGAGGGGTATTATAATTTACGAAGAAGACTKATGATATCTGCGAGGATGTTTGTGGAGAGCAGGGAACACCGGCAGCACTGAACATGTCGAGACTGAAGTGTATTTTGCGAGGATTGGACTTCAAGACTTACATGTTTACGTTTATTATCATCCCAATAGGTGTCTTTTTAATCTATTTGCACGGACAGAAAATTACATACTTTTTTAGACCATTGTGGGAAT
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01086 Ps_contig_mira-and-tgicl-ass_franssen_9899
SEQUENCE=CAGACTTCTGTGCCCAGTACAGCYTAAGATTTGAACTGCCTGTTGTTGAACCTCCATCTGACATAAGAATAGTTATTGAGAGGATCGTTGAGTTCATCTTAAAAAAATGGCAGGCCATTTGAGGCCGTTCTTGCTGAACAGGATCGTGCTCATGGAAGGTTCCCATTTCTTCTGCCTTCTAATCAGTATCACACTTACTATCTGAAAGTTCTTCAAACTGCTGAAGAGTCTAAATTACCAGGTAAGGGCTACCAAAAGTACAATCCAGCTGGTCGTGCAAGAGATAATAACACTGCTGCATATGAAGAAAATGACAATAAATTCAAAATGATTATTGGCAATTTAAAGAAGGATGGTCAAGAGCCAACTCCCAAAGACTGCCAATCTCAAACTACTGTTAACATCCATGCGGCGGCAGCTGCTGCTATTCTTCAGGCAGCTACTAGGGGTATTAAAGGCCCAATTTAGAAATTCTCAGTAAGACGTCAAGTGGTAATGGTCAAGGCTATTGYAGTCTGAACGCAGAGACTAGTGCTTCTGTTCCAGTTGCCAAGGCTATTGCAGAAAAAGTGGCTATTGCAGCAGCAGGTGAGGCTGACTCCTCTGAAGCACATATGACTAAAGAGCAAAAGCTTAAAGCAGAGAGATTGAAACGAGCCAAGATGTTTGCAGCCATGAATAAAAAGTGGAGCTGGAGCACTTAAAAGTGAACTGCCAAGAGCTTTATCTCTTGAACCACCAGGCTCTGGGCTTTCAGGTTCAGATGCTGAGATTGGAAATCTTGTGAATAAAGAAAGGGAAGGAAGTTCTGTTCCATTCAATGCTGATAACTCAGATAAAAGTCATAAGTCTGAAGAAAAGCTTTCTGATAATAATTCAGATAGAAGTAACAAGTCTGAGGAAAAACTTGTTGATAATAATATTGAAAGGCGATCTAAAAGAAAGTACCGATCAAGGTCCAGTAGACATGAAGAAGAAGAAGAGGAGGAGGAAGAGGAAGAAGAAGAAGAAAATAAAGAAGATATAAGGAATCAGARAACGATCCAGAAAA
TARGET=970,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01087 Ps_contig_mira-and-tgicl-ass_franssen_9899
SEQUENCE=CAGACTTCTGTGCCCAGTACAGCYTAAGATTTGAACTGCCTGTTGTTGAACCTCCATCTGACATAAGAATAGTTATTGAGAGGATCGTTGAGTTCATCTTAAAAAAATGGCAGGCCATTTGAGGCCGTTCTTGCTGAACAGGATCGTGCTCATGGAAGGTTCCCATTTCTTCTGCCTTCTAATCAGTATCACACTTACTATCTGAAAGTTCTTCAAACTGCTGAAGAGTCTAAATTACCAGGTAAGGGCTACCAAAAGTACAATCCAGCTGGTCGTGCAAGAGATAATAACACTGCTGCATATGAAGAAAATGACAATAAATTCAAAATGATTATTGGCAATTTAAAGAAGGATGGTCAAGAGCCAACTCCCAAAGACTGCCAATCTCAAACTACTGTTAACATCCATGCGGCGGCAGCTGCTGCTATTCTTCAGGCAGCTACTAGGGGTATTAAAGGCCCAATTTAGAAATTCTCAGTAAGACGTCAAGTGGTAATGGTCAAGGCTATTGYAGTCTGAACGCAGAGACTAGTGCTTCTGTTCCAGTTGCCAAGGCTATTGCAGAAAAAGTGGCTATTGCAGCAGCAGGTGAGGCTGACTCCTCTGAAGCACATATGACTAAAGAGCAAAAGCTTAAAGCAGAGAGATTGAAACGAGCCAAGATGTTTGCAGCCATGAATAAAAAGTGGAGCTGGAGCACTTAAAAGTGAACTGCCAAGAGCTTTATCTCTTGAACCACCAGGCTCTGGGCTTTCAGGTTCAGATGCTGAGATTGGAAATCTTGTGAATAAAGAAAGGGAAGGAAGTTCTGTTCCATTCAATGCTGATAACTCAGATAAAAGTCATAAGTCTGAAGAAAAGCTTTCTGATAATAATTCAGATAGAAGTAACAAGTCTGAGGAAAAACTTGTTGATAATAATATTGAAAGGCGATCTAAAAGAAAGTACCGATCAAGGTCCAGTAGACATGAAGAAGAAGAAGAGGAGGAGGAAGAGGAAGAAGAAGAAGAAAATAAAGAAGATATAAGGAATCAGARAACGATCCAGAAAA
TARGET=997,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01088 Ps_contig_mira-and-tgicl-ass_franssen_9939
SEQUENCE=TCGGGGGTGAATCATRTTATTATTATAGATTATTCTTAGGTACATACATACATGAATAAAGTCATATACACACAGCTACTACCAACCAACCCACATGTTTTTTTCTCTACGATTTTATAATATATAAAACTCAGAATCATAAAGATAAAGATAGTTAATTATCACAACAAGAAGGGGAAAAATGGATTCTAGAAAGTTTCAAATTCAAGCAATTTCGGATCCTAACTCGTCATCCACTGACGTTTATCATCTTGCTTTGGATATTGGAGGATTCTCTTTGACAAACTGTATACTCANAAAG
TARGET=41,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01089 Ps_contig_mira-and-tgicl-ass_franssen_9958
SEQUENCE=TCCTTTCAAATATCTAAAAATAGGATTGATAAGATTGATAGTGACAACTTGGTTTGCAACTACACAACGATTGAAGGAGATCCATTAGGTGACAAGCTTGAGACAATAGCTTATGAGGTTAAGTTTGAGGCCATTAATGATGGAGGTTGTCTTTGTAAGATGACAAGCAAATACAATGCTATTGGAGAGTTTGAAGTCAAAGAGGAAGAGATCAAGGAAGGAAGGGAAAGCTCTATTGGAATTTGCAAAGTTGTTGAAGCCTATTTGCTTGAGAATCCACAAATCTATGTTTAATTAGTTATTTTTAATTCATTTATTTTAATTTGTCAAAAAGTATATATATATACACTCATCTTATCTTATTTAAAGATGAAATACAAATT
TARGET=214,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1340
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.102276
PRIMER_LEFT_SEQUENCE=GGAGATCCATTAGGTGACAAGC
PRIMER_RIGHT_SEQUENCE=GTGGATTCTCAAGCAAATAGGC
PRIMER_LEFT=75,22
PRIMER_RIGHT=279,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.1781
PRIMER_LEFT_1_PENALTY=0.075795
PRIMER_RIGHT_1_PENALTY=0.102276
PRIMER_LEFT_1_SEQUENCE=CGATTGAAGGAGATCCATTAGG
PRIMER_RIGHT_1_SEQUENCE=GTGGATTCTCAAGCAAATAGGC
PRIMER_LEFT_1=67,22
PRIMER_RIGHT_1=279,22
PRIMER_LEFT_1_TM=59.924
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.4256
PRIMER_LEFT_2_PENALTY=0.323355
PRIMER_RIGHT_2_PENALTY=0.102276
PRIMER_LEFT_2_SEQUENCE=GCAACTACACAACGATTGAAGG
PRIMER_RIGHT_2_SEQUENCE=GTGGATTCTCAAGCAAATAGGC
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=279,22
PRIMER_LEFT_2_TM=59.677
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=0.4959
PRIMER_LEFT_3_PENALTY=0.031769
PRIMER_RIGHT_3_PENALTY=0.464167
PRIMER_LEFT_3_SEQUENCE=GGAGATCCATTAGGTGACAAGC
PRIMER_RIGHT_3_SEQUENCE=CTCAAGCAAATAGGCTTCAACA
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=272,22
PRIMER_LEFT_3_TM=59.968
PRIMER_RIGHT_3_TM=59.536
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=0.5400
PRIMER_LEFT_4_PENALTY=0.075795
PRIMER_RIGHT_4_PENALTY=0.464167
PRIMER_LEFT_4_SEQUENCE=CGATTGAAGGAGATCCATTAGG
PRIMER_RIGHT_4_SEQUENCE=CTCAAGCAAATAGGCTTCAACA
PRIMER_LEFT_4=67,22
PRIMER_RIGHT_4=272,22
PRIMER_LEFT_4_TM=59.924
PRIMER_RIGHT_4_TM=59.536
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=206
=
PRIMER_SEQUENCE_ID=SSR_01090 Ps_contig_mira-and-tgicl-ass_franssen_9958
SEQUENCE=TCCTTTCAAATATCTAAAAATAGGATTGATAAGATTGATAGTGACAACTTGGTTTGCAACTACACAACGATTGAAGGAGATCCATTAGGTGACAAGCTTGAGACAATAGCTTATGAGGTTAAGTTTGAGGCCATTAATGATGGAGGTTGTCTTTGTAAGATGACAAGCAAATACAATGCTATTGGAGAGTTTGAAGTCAAAGAGGAAGAGATCAAGGAAGGAAGGGAAAGCTCTATTGGAATTTGCAAAGTTGTTGAAGCCTATTTGCTTGAGAATCCACAAATCTATGTTTAATTAGTTATTTTTAATTCATTTATTTTAATTTGTCAAAAAGTATATATATATACACTCATCTTATCTTATTTAAAGATGAAATACAAATT
TARGET=335,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01091 Ps_contig_mira-and-tgicl-ass_franssen_9982
SEQUENCE=AGTTTTTTTTTAAGTTTACTAACTTAGAATTTTCTTAAGTCACTAGCAGAGAAAGTAGATGTTATAACATTCATATATTTACWTAGAAAATTGTAAATGTATTTCAACCTATTTTTATTACATTCAATTATACWAAAGAATAGTATGAATATATATATATATATAGAAATTTCATGTACACTATAGAGTAAATAGACATAGAAAGTAACTTTTGTGAACAATCTTAAACAAGGAAAAATTATTGAAAGTGGAAACAATTCACACTTTGTTATTGTTTTTTTATTATACAGCCTCAACTTCATAGGACCTTAATGCCATTGTTTTTCATAACCTGAGAGTTTGAATCAAGAAGTGTTTCCAGTGATCTTTTCGACCTTCGGTTTCTTGCTCTTCCTGCTTCTTTTAACAACTCGGCCAACCTACGTTTCTCATCGTCGACATCAGGGTTTGCTGTTCCAAGCTTCTCTTCTCTCATGCCAACTACATATTCCAGCATTTCAATGGCATCATCCACTCTTCCCATTGCATCATAGGTGCCTGCAAGATTGCTATAGACTCCTAATGTATCTTGATAGAAGTAACGGGTMCMTACTTCTTTTTACCAATATAGTTCTAGCCTCTTACAAACAAT
TARGET=149,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01092 Ps_contig_mira-and-tgicl-ass_franssen_9988
SEQUENCE=AACAAACAAACCGACAAACATATAATCTCGGTAATTGGRTGATGTAGTAATGCAGAAACTCCAAATTAATTACGCACACCAAGAAGAAATTGCAACAACAACAACTTTATATAATGTTAAATAACAAAAGACAAAGCTGGAA
TARGET=93,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01093 Ps_contig_mira-and-tgicl-ass_franssen_9989
SEQUENCE=ATACACAACCATTCTCTATAATATTTACATAGATCAAATTGTGAAATAATGCAACACAATGAACATGAAGTCGGCCAAACATCAACTTAATTCAACTCGTTTATATAATTTTTTCTTTAACTATCCGAGCTAGAAGGCAAAACATCATGACACAAAACAAACAAACCGCAAACATATAATCTCGGTAATGGATGATGTAGTAATGCAGAAACTCCAAATTAATTACG
TARGET=155,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.4325
PRIMER_LEFT_PENALTY=0.102129
PRIMER_RIGHT_PENALTY=2.330336
PRIMER_LEFT_SEQUENCE=AACACAATGAACATGAAGTCGG
PRIMER_RIGHT_SEQUENCE=GCATTACTACATCATCCATTACCG
PRIMER_LEFT=52,22
PRIMER_RIGHT=205,24
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=59.670
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=8.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=3.0316
PRIMER_LEFT_1_PENALTY=0.701300
PRIMER_RIGHT_1_PENALTY=2.330336
PRIMER_LEFT_1_SEQUENCE=CGGCCAAACATCAACTTAATTC
PRIMER_RIGHT_1_SEQUENCE=GCATTACTACATCATCCATTACCG
PRIMER_LEFT_1=71,22
PRIMER_RIGHT_1=205,24
PRIMER_LEFT_1_TM=60.701
PRIMER_RIGHT_1_TM=59.670
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=3.2073
PRIMER_LEFT_2_PENALTY=0.876993
PRIMER_RIGHT_2_PENALTY=2.330336
PRIMER_LEFT_2_SEQUENCE=AAGTCGGCCAAACATCAACTTA
PRIMER_RIGHT_2_SEQUENCE=GCATTACTACATCATCCATTACCG
PRIMER_LEFT_2=67,22
PRIMER_RIGHT_2=205,24
PRIMER_LEFT_2_TM=60.877
PRIMER_RIGHT_2_TM=59.670
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=3.2329
PRIMER_LEFT_3_PENALTY=0.902591
PRIMER_RIGHT_3_PENALTY=2.330336
PRIMER_LEFT_3_SEQUENCE=CAACACAATGAACATGAAGTCG
PRIMER_RIGHT_3_SEQUENCE=GCATTACTACATCATCCATTACCG
PRIMER_LEFT_3=51,22
PRIMER_RIGHT_3=205,24
PRIMER_LEFT_3_TM=59.097
PRIMER_RIGHT_3_TM=59.670
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=3.7071
PRIMER_LEFT_4_PENALTY=1.376776
PRIMER_RIGHT_4_PENALTY=2.330336
PRIMER_LEFT_4_SEQUENCE=AGTCGGCCAAACATCAACTTA
PRIMER_RIGHT_4_SEQUENCE=GCATTACTACATCATCCATTACCG
PRIMER_LEFT_4=68,21
PRIMER_RIGHT_4=205,24
PRIMER_LEFT_4_TM=59.623
PRIMER_RIGHT_4_TM=59.670
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_01094 Ps_contig_mira-and-tgicl-ass_franssen_9998
SEQUENCE=GCACGACTTGGTTGGAGATAGATGGAATTTGATAGCAGGTCGCGTTCCAGGTCGCAARGCAGAAGAAATTGAGAGATTCTGGACTATGAGACAGTGCTCCTCATGCCAACAGAAATCATCAAAGTAAAGCAAATTAAATTTAGGATTAAGAATCTTGTTTTGGTTATTCAGATTATTATTATTTTTGGAATAGGGTTTATTTTGAATTATTCGTAAYTACCTCTCTAGTTGAAAGTAG
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01095 Ps_contig_mira-and-tgicl-ass_franssen_9999
SEQUENCE=TGTTTTGGATATTCAGATTATTATTATTTTGGAATAGGGTTTATTTTGAATTATTCGTATTACCTCTCTAGTTGAAAGAGTTTGAATTTTATTAGTTTTGTATACTTTGTACCCACAAAATAATATATCTATTTAAGTATATTAATTAGATCCTTTAAGTT
TARGET=17,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01096 Ps_contig_mira-and-tgicl-ass_franssen_10025
SEQUENCE=TTTATTTCTTATTTGTAATTATTCACATTTCTACTTGTTCTCTTGGATACAATACTATGCTAAGCTGAGTTCACATTTCATTATTTCCACACACACACTCAATACAATTAGGTTTAACCAAAAAGCAAATATAC
TARGET=88,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01097 Ps_contig_mira-and-tgicl-ass_franssen_10046
SEQUENCE=GAATGTGAAATGGAGTTACTCTTCTTCTTCTTCTTCTTCACAGTTCCAAGTTCAGACAAACCAAAACCCCTTCTTCACTCCTCACACAGATCCACAACCATCTTTCTCTCTCCAACACTTCATTCWCTGAAGTGAGGATAGTGTATGATAAAGACAGTGGTAGAGCTAGAGGCTTTGGATTTGTTATCTTTTCAAATGAAGATGATGCAAA
TARGET=20,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01098 Ps_contig_mira-and-tgicl-ass_franssen_10092
SEQUENCE=CCGGGGGCTCTCTCGTTGGGTTGCATTCTATTTCTACTTTTCTTTAACTACTTCTTAATAAACAACAACTTCATAACCATTTCTTTCTTCTTCTTTCSAGCGAAAGAAAGAAAGAAAATGGGTTGTTTTCTTCAGTGTTTTGGTTTAACCACTAAAAGAAAACGCAGAAAGACTCTTTACAAAGTCCTAGCTCGAGAAGACCAGAAATATGGAAACTATGAAGTTCTTGCTATTACAGAAAAAGCAATTATCCCTTATTCTGAATCCAGAGGTAGAGATCAAGAAAAGGAGAAGAGTGGTGTTAAACCCAAGAAGAAGAAAGTTAGCTTTAATTTGAATTTACAGATATATGAGGCAAACCCTAGTTCTTATCAGGTGTTGGAGGATGAGGAAGAAGAGAATAAGGAC
TARGET=102,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01099 Ps_contig_mira-and-tgicl-ass_franssen_10093
SEQUENCE=CGTCAAACACAGTAACTTGTTGGAGCCAAACGGCTTTAAAACTTCTCACATGTTYCTTCTCTCTCTCYCTCTCTCTCTGGGTTGCATTCATTTCACTTTCTTTAACTACTCTTAATAAACAACAACTTCATAACCATTCTTTCTTCTCTTTCCAACAGCGAAAGAAAGAAAGAAAATGGGTTGTTTTCTTCAGTGTTTTGGTTTAACCACTAAAAGAAAACGCAGAAAGACTCTTTACAAAGTCCTAGCTCGAGAAGACCAGAAATATGGAAACTATGAAGTTCTTGCTATTACAGAAAAAGCAATTATCCCTTATTCTGAATCCAGAGGTAGAGATAAGGAGAAGAGTGGTGTTAAACCCAAGAAGAAGAAAGTTAGCTTTAATTTGAATTTACAGATATATGAGGCAAACCCTACGTTCTTTATCAGGTGTTGGAGGAGATGAGAGGTTGG
TARGET=58,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01100 Ps_contig_mira-and-tgicl-ass_franssen_10093
SEQUENCE=CGTCAAACACAGTAACTTGTTGGAGCCAAACGGCTTTAAAACTTCTCACATGTTYCTTCTCTCTCTCYCTCTCTCTCTGGGTTGCATTCATTTCACTTTCTTTAACTACTCTTAATAAACAACAACTTCATAACCATTCTTTCTTCTCTTTCCAACAGCGAAAGAAAGAAAGAAAATGGGTTGTTTTCTTCAGTGTTTTGGTTTAACCACTAAAAGAAAACGCAGAAAGACTCTTTACAAAGTCCTAGCTCGAGAAGACCAGAAATATGGAAACTATGAAGTTCTTGCTATTACAGAAAAAGCAATTATCCCTTATTCTGAATCCAGAGGTAGAGATAAGGAGAAGAGTGGTGTTAAACCCAAGAAGAAGAAAGTTAGCTTTAATTTGAATTTACAGATATATGAGGCAAACCCTACGTTCTTTATCAGGTGTTGGAGGAGATGAGAGGTTGG
TARGET=69,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01101 Ps_contig_mira-and-tgicl-ass_franssen_10093
SEQUENCE=CGTCAAACACAGTAACTTGTTGGAGCCAAACGGCTTTAAAACTTCTCACATGTTYCTTCTCTCTCTCYCTCTCTCTCTGGGTTGCATTCATTTCACTTTCTTTAACTACTCTTAATAAACAACAACTTCATAACCATTCTTTCTTCTCTTTCCAACAGCGAAAGAAAGAAAGAAAATGGGTTGTTTTCTTCAGTGTTTTGGTTTAACCACTAAAAGAAAACGCAGAAAGACTCTTTACAAAGTCCTAGCTCGAGAAGACCAGAAATATGGAAACTATGAAGTTCTTGCTATTACAGAAAAAGCAATTATCCCTTATTCTGAATCCAGAGGTAGAGATAAGGAGAAGAGTGGTGTTAAACCCAAGAAGAAGAAAGTTAGCTTTAATTTGAATTTACAGATATATGAGGCAAACCCTACGTTCTTTATCAGGTGTTGGAGGAGATGAGAGGTTGG
TARGET=160,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01102 Ps_contig_mira-and-tgicl-ass_franssen_10101
SEQUENCE=GAATTTTTTGTCATGCCCCCTTCCATCTGTCATTCCTCTCCACAATCATCCTGGAATGACGGTCCTAAGCAAGCTCATATATGGTACAGTGCATGTTAAATCCTATGACTGGATCGATTTCCCTGGCCCAAATGATCCAACTGAAGCTAGAGCTGCAAAGCTTGTGAAAGATAAAGACATGACAGCACCGACTTCAACAACAGTTCTTTATCCTACCGTTGGAGGAAACATTCATTGTTTTAAGGCCATTACTCCTTGCGCAATCTTTGACATATTATCTCCACCATATTCACCAGAACACGAAAGACACTGCACTTACTTCCAACAATCTCAAAGAAAAGATCTTCCAGGTAATGTTGAGTTGGATGGAGTTACATTTTCTGATGTGACATGGCTAGAAGAATTTCAACCGTCGGATGAGTTTGCAATAAGAAGAGGACTGTACAAGGGTCCAGTTATTAGAACATATTAACAAGTTTCTAGTTTTTCTATATATTTACATTTGAGAAGAAGTCATGAAAATTGTGGAAGAAGTCATGAAAAAAAGGCAATGCAATTAATTTTATTATTTATTTTTTGGTCATTGTTAGTTATTATTATTATTATTATTTTTA
TARGET=591,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01103 Ps_contig_mira-and-tgicl-ass_franssen_10191
SEQUENCE=TCTCGACATAGTTTCCGATCCTCACGTATGAACAATCACATAACCAACAACAATGAACGCGATACTCACCTCGACGTCCGGTATGGTCTTCAGCTCTTGGTTGATCAGGATCTGTGTGCGGGCGTGACGTTATCGTTGAAGAGCAAATGATGGTTCGTTGTCGATTAAGGATGRAGGAGATGGATTCTTTCGGGTGCGCCTCCGTCGTCGAATACAGATGAAGTTGGAGGCCCGTTGATTTCGCAACGAGGGATGACGGAACCTCGGCGTTACGAAGAATCCGATAGTTATCGCGTAGTGAAGCGTTGTTGATGAACGATGAAGAATTAAAATCGTTCCCTCGTTCTTCTTGAACCCTCGATTATTAGAAGGTGGGCGTACTTTAACTCTGTTTTCTTCTTCTTCAACTTTGTTCGCGGATCCGTTTTCTGTTTCGTTGTTCTATGGATTCGTGTTGTTGTA
TARGET=394,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01104 Ps_contig_mira-and-tgicl-ass_franssen_10192
SEQUENCE=ACTTCATCGTCTCAAACTTCTAACTTCATTCACCCTTCGTCGTCCTCCATAACCAATTTCTTCGACCTCATTATCCATCTTCTCYCCACTCTTCTTCTCACGCGGCTTCACCCTTCACCTTACAAACAGAATCCAAAGGAAACCTTCCATTTGGTGAAAACACCTGCAACCATAACTCAGTTCAGAATCCTTTCCTCAAATATACAACAACACGAATCCATAGAACAACGAAACAGAAACGGATCCGCGAACAAGTTGAAGAAGAAGAAACAGAGTTAAGACG
TARGET=258,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01105 Ps_contig_mira-and-tgicl-ass_franssen_10199
SEQUENCE=GATTGTCATTGTTGTTTSGAAAACACCATGTGTTCTTCTGCGTTTGGAATTTGAAACAGAACCACCAACTTTTATTCATTCCTTCATCCGTACACACTTCTAATTCAAATCCTAATCATAATCTAGGGTTCCTCTCTCTCTCTCATGACTTCAGTTCTCAATTATTCCTCAATTCAACTACCTTCTTCTCTCACCTTCNTCATCCACCATCCTTATTTTGAGA
TARGET=132,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01106 Ps_contig_mira-and-tgicl-ass_franssen_10222
SEQUENCE=TACTTTCTTGTTTTTTCTATTGTAAATAACTTTTGGKCCTCTCAAGCACATTGGACTTGTGTAGATCATGGGTTTTATTTGTTTTCTCTGCTGATAATACAATACCTTCGTTTTTCCTAGGTCCATAGATCCTTAGAAGAATAGCAACCAACTCCTATAATGTCCACACGAGCAGGTTCCTTTGTTATGATTCGCAAGGTAGGTAGGTCCTATTCTTTATTATTCACATACAATAATAGTGTTGAACTTTCATACAAACAGAACGCCACCCACATGTG
TARGET=197,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01107 Ps_contig_mira-and-tgicl-ass_franssen_10253
SEQUENCE=GATAAACGTCCCCCTTTCATATATAACACCTCTATCTCTTCTTTCCTTCACGCTCCCAATTCCAGATTTTCTTTCTTCCTCCCTCATGCGTAACACAATGCGGAGATAGCGATTCTCTCTCTCTCTCTCTCTCTCTCAGATCTGATTCTCTCCGAATAGCCTAAGGTCTAAACTATCTGTAAGGCACTTGACCTGCTGACAAGTGGCAACATGGCGCAAGACGAAATATTCTAGAATAGAATAACAAACGGTCTACATCGGGTTACTGTTCG
TARGET=114,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5372
PRIMER_LEFT_PENALTY=0.408061
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=CCAGATTTTCTTTCTTCCTCCC
PRIMER_RIGHT_SEQUENCE=CCCGATGTAGACCGTTTGTTAT
PRIMER_LEFT=61,22
PRIMER_RIGHT=261,22
PRIMER_LEFT_TM=60.408
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=201
PRIMER_PAIR_PENALTY_1=0.5916
PRIMER_LEFT_1_PENALTY=0.408061
PRIMER_RIGHT_1_PENALTY=0.183585
PRIMER_LEFT_1_SEQUENCE=CCAGATTTTCTTTCTTCCTCCC
PRIMER_RIGHT_1_SEQUENCE=ACAGTAACCCGATGTAGACCGT
PRIMER_LEFT_1=61,22
PRIMER_RIGHT_1=268,22
PRIMER_LEFT_1_TM=60.408
PRIMER_RIGHT_1_TM=59.816
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=0.6472
PRIMER_LEFT_2_PENALTY=0.408061
PRIMER_RIGHT_2_PENALTY=0.239177
PRIMER_LEFT_2_SEQUENCE=CCAGATTTTCTTTCTTCCTCCC
PRIMER_RIGHT_2_SEQUENCE=CTAGAATATTTCGTCTTGCGCC
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=233,22
PRIMER_LEFT_2_TM=60.408
PRIMER_RIGHT_2_TM=60.239
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.7030
PRIMER_LEFT_3_PENALTY=0.408061
PRIMER_RIGHT_3_PENALTY=0.294975
PRIMER_LEFT_3_SEQUENCE=CCAGATTTTCTTTCTTCCTCCC
PRIMER_RIGHT_3_SEQUENCE=CAGTAACCCGATGTAGACCGTT
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=267,22
PRIMER_LEFT_3_TM=60.408
PRIMER_RIGHT_3_TM=60.295
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=0.8994
PRIMER_LEFT_4_PENALTY=0.770341
PRIMER_RIGHT_4_PENALTY=0.129103
PRIMER_LEFT_4_SEQUENCE=GCTCCCAATTCCAGATTTTCTT
PRIMER_RIGHT_4_SEQUENCE=CCCGATGTAGACCGTTTGTTAT
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=261,22
PRIMER_LEFT_4_TM=60.770
PRIMER_RIGHT_4_TM=60.129
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_01108 Ps_contig_mira-and-tgicl-ass_franssen_10256
SEQUENCE=CTTTTCTAGAGTATTCCAAATTTGGGTTGAAGTTTGTTCCGTACAGTCCAAAGAAAGTTCTCGACCAACCTATCTCTAGCAAAGCTCAACTTTTCTCCAAGGCCTGTTCTTTTCCACCATCTTATAATTAGAAAGGACAAAAAAAAATTAAGCATTAGAACGAAAATGTTCTCTCTCTCGTGGCATTCAAAATATGTAT
TARGET=170,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01109 Ps_contig_mira-and-tgicl-ass_franssen_10259
SEQUENCE=GCTCTCTCTCTKAATTTATTCAAACCTCTCTCTACTAAAAATCATAAACATGGAGGAACGACAGCCCGAACAAGAACAACACTGCCAGGTCATCACTCTCGGAACTCATAACCCTCTCCTCCCCGGGCCCCAATCGGACGATGAAGACGACGGCGGATGCGCCTATTCAAGGGCTGTTCTCGTTGTCGACATGGTCTGGAACCTGCCTTCGTGCTTGTCGCCGCCGCCGTCATCCTATCCACGTTCGCCGAGCCGTCCCTCCACTCCTCTGAGTTACG
TARGET=2,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01110 Ps_contig_mira-and-tgicl-ass_franssen_10275
SEQUENCE=GAAAATATAAAACGGTGTTTTTGAATCSGGATCKAGCGCCTTCCATTCACCTCTTCAAAAACCCTATTTTTTATTCATTCTCTATCTTCTTCTTCTTCTTCCCTTTGCTTTTCTGTGTTCTCGATTCGATTCACCACAATGGCCAAAGGAGCTAAGAGAAAGACCAAGCAAACTGAAGATTCCAAACCTGATTCCGAACCTGCTACAGCTACAGGTTCATCGNAAAGAAAACGAAGCCAAAATCAAAATAACCAAAGCCGCCCTACCCAAAAGCAAAACG
TARGET=85,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01111 Ps_contig_mira-and-tgicl-ass_franssen_10279
SEQUENCE=CTTTTTACTTTTTCTATATATATAACTAGCTTTGATTCATCTCGTTTCCTTATCGCAATCTGAACCGAGGAATGTTCTTCTTTAAGCGNTAAAATCGACGTTTGGAACATTCCGTAAGCGTCGACGAAGCGGAGAGAATACTCTAGAAGCTTCTCGACCCAAGCAGGGTGGTAGCGAAGAGACTCTTGAGTTTGCGGAAGTTGGAGAATGTCTTGAAAAGTTTCATGAGTTTGTTTGAGAAGTSYTAAGCCGTTGGATAGGCTTTTGGATGTTAATGGATTCGATTTCGTAGAATTAGTCCAGGATCTGAGACCGTTGATCTCATCCTTGATCTGAGAAATCAACGGATGGGATCTGCAGGGAAGACTTATGGATCTTATGTAGGTGTGATATTGATGGCTTTTGNGATGGACGGTTAGGTTA
TARGET=15,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01112 Ps_contig_mira-and-tgicl-ass_franssen_10287
SEQUENCE=ACAAGAGGATGAATGGTTCCAACTTTAATTCTTTCTAGCCATATTGAATTTGATGCAGTTTACGAAGCCATAGATGACGATGATGATGATGGATGGAGCCGGAGCTTTGGAGAGCCGAGGCCACGGGAACATCCACAGGAAGAGGAACTTCAGAAATTCAAGAAAGAGTTTGAAGCATATCAAGCAGCGAGGTTTCAAGAATGTTCGAGGTTTGCTGAAATCGGCAGTGTTTCTAGCCGATTAATTTTA
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5182
PRIMER_LEFT_PENALTY=0.497481
PRIMER_RIGHT_PENALTY=0.020747
PRIMER_LEFT_SEQUENCE=ATTTGATGCAGTTTACGAAGCC
PRIMER_RIGHT_SEQUENCE=GTTCCTCTTCCTGTGGATGTTC
PRIMER_LEFT=47,22
PRIMER_RIGHT=147,22
PRIMER_LEFT_TM=60.497
PRIMER_RIGHT_TM=59.979
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=0.6070
PRIMER_LEFT_1_PENALTY=0.497481
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=ATTTGATGCAGTTTACGAAGCC
PRIMER_RIGHT_1_SEQUENCE=CCTCGAACATTCTTGAAACCTC
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=209,22
PRIMER_LEFT_1_TM=60.497
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.6076
PRIMER_LEFT_2_PENALTY=0.497481
PRIMER_RIGHT_2_PENALTY=0.110131
PRIMER_LEFT_2_SEQUENCE=ATTTGATGCAGTTTACGAAGCC
PRIMER_RIGHT_2_SEQUENCE=GAAGTTCCTCTTCCTGTGGATG
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=150,22
PRIMER_LEFT_2_TM=60.497
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=0.6076
PRIMER_LEFT_3_PENALTY=0.497481
PRIMER_RIGHT_3_PENALTY=0.110131
PRIMER_LEFT_3_SEQUENCE=ATTTGATGCAGTTTACGAAGCC
PRIMER_RIGHT_3_SEQUENCE=TGAAGTTCCTCTTCCTGTGGAT
PRIMER_LEFT_3=47,22
PRIMER_RIGHT_3=151,22
PRIMER_LEFT_3_TM=60.497
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.6908
PRIMER_LEFT_4_PENALTY=0.670093
PRIMER_RIGHT_4_PENALTY=0.020747
PRIMER_LEFT_4_SEQUENCE=GAGGATGAATGGTTCCAACTTT
PRIMER_RIGHT_4_SEQUENCE=GTTCCTCTTCCTGTGGATGTTC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=147,22
PRIMER_LEFT_4_TM=59.330
PRIMER_RIGHT_4_TM=59.979
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_01113 Ps_contig_mira-and-tgicl-ass_franssen_10306
SEQUENCE=CTCTCTCTTCTCTCTCTCCTAATACGATGCTATTTTCTTCGATTTTCTTAGAAACCTCAGAGCTGCCTTCAATGGATGTCGCTGCTATCTCTCTATTCGAGACCACAACCACCGCTTTCTTCTCTCTCACTCGCATAGAGACTCTACTCAACAGGTTATGAGAGGTTTAATTTTTTCAAAGGGCTTGCCAGCGTTGTATAGTAGTAGATATCAAATTCAATCATCAAAGCAGCTCTCTAAT
TARGET=9,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01114 Ps_contig_mira-and-tgicl-ass_franssen_10311
SEQUENCE=GACTGTAACCCTACCTCCACTCAAACGAMMCCTTCGTTTTCCTAACCCAAAACCAACCGTCCAGAGCTCACTGCAGCTACGCTGTGAATAGCTACTTCCAGAAGAAGGGCCAAGCACCAGGAACTTGTGACTTTGCAGGAACAGCTACACCTGTTGCATCTGACCCAAGTGTTAGTGGCTGTGTCTACCCTTCAAGTGCCAGTGGTGCAGGCACTAGCACAACAACCCCATCGTCAGGCACCACTACACCCTCCATGGGAACCGGCACTACCCCTTCAACAAGCACCGGCACCGGCACCGGCAGTGGTATGGGCACCGGCACAGGAACAGGAACAGGCACCAGCACCGGCACCGGCACGGGTACGGGTACGACTCCATACAACACATCTCCTGGAGTGTTAGGAGGTATTGGAACTGGTATGGGGCCTTCTGGAGCTGGAGGAATGAATGATGATAGTCATGGTGGGATTAGGCTTTTGGACACTAGTTTCTTATCAATCCCACTCTTTTCTATTTTCATTATGTTTTGGTAGGGTTAAAAAGTTTATATGTCAGTGTATGAATGGGTGTTATTTTCTAGGTATTGAACACCGAGATGTTACTAGTTCACTTTTTTTGTTTATTTTTCTCTCCACCTAATTCTGCCATTTCTTGTGTTGGTTTTTAAAAAGATGGGCAGTTGATTAATTAATTAATTTTGATGAGTACTCCTATTAAGGTCCCTTAGTCAGTATAATAAAGTTTWAATACTTGTCATTTCAAGGAACCTTACGTTTGTAAACCTCTCTTGTATCATCCATCAAAGTGTTGAAACTTTGGAG
TARGET=683,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01115 Ps_contig_mira-and-tgicl-ass_franssen_10352
SEQUENCE=GGTTGATAATCGCTTTTTAGTATTACAAATGGCCTTTACAAGTGTTATAGCNCTTTTTGCTTTTCTTCTTCTTCAACCTCTTTTAGCTAAAAGTGACYTGCTTTCTCCTTTGTTTTCTCCAATCTTTGATGATGTGTGCAAGGAAGTTGAATGTGGGAAAGGAACATGCAAAGCTTCTAAGAATGGTGTTATTCCTTTTGAATGTGAGTGTGATCATGGTTGGAAGAAAGCTTTTGATTCCAA
TARGET=63,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01116 Ps_contig_mira-and-tgicl-ass_franssen_10354
SEQUENCE=GTCGGGAGCCGAACAAACTCTCCAACGTCTCTCACACTCAGCAGAAAAATCCAAAGAATCTCTCTCTCCCTCATTCCATTCCCTCTCCCTCTCACTACCATAAATCCACATGTCACTCATACCTACTCCACCCTCATCATACAAAAAAATTTCTCACCAAATTCCTCATTCTCACTTCTCTCTCTGACAAAAGTAAACTTCCACCTTYATTCTCTCAA
TARGET=59,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01117 Ps_contig_mira-and-tgicl-ass_franssen_10355
SEQUENCE=CGATTTTAATTTCTTTCGAGCCTCCTTGTAACCTTCCTTTCCCTACTTAATTTGTKTCCYACGTCCTGCTTCCCGGAAGCTTGAGACATTCTCTCGTGCTCTTGAAGTGATTCTTCCACTCTCTGAACAGCTGCCCTAGCATTATCTATTTCTGAACGAGCGAAAGCTCTTTCCTCATCAACAAGTTTCTTAGCTTCTTCAGAAGCCTGCTTAAGAAAACTTGCCAATTTCTTCACCTCCGCTTTCTCTTGTATTAGTTCCCCTTCCCTTTGAGTTAGTTGAACTGCTAGAGCTTCAACCATAGCAATAGCATCGTCGACATCATCCATGTTTCTACCCGCCACACGTCCCTTTAACGATTCAAGAGAATCTCTGAGCTTCTTTAAAAGAACGTGTTTTTCCAATGATGCTGCTTCTCTAAGTCTGGCCTCTTCCGACAACTTAGCAGCAGCCGCCAAACCCTTCTCAAATTTACTAGCAAGATCACGAACCGAAAGACGATTATGCTGATCCAACAACTGAGCAGTCTCGCGCGCAATAACCTCCTTCATAGATAACAGTTTTGGGTCATCCTTTGTCTCCATAATCTGATTGTTAGCTCCGATTCTGTAATTAGGAAACCGACTAGAAGCAAATATCACATCAGAAGATACCGCAGGCACTGCCTCTTTTTTCATAGTATCTCCAAAATCACGTGTTAACCTCGTCATCTCTTACAACCAATTCCCTAAGAGATCATGAGAAGATGAAAATGGTGGTTAGGGTTGTGATTGTTGAAGTAGATGCAAAAGATTGCTCCAATTTGGTTATTCAACACAGGAATCACTGGTCTTGTTGATGATGCTTTTAGAGAGAGAGAGAGAGAGAGATTGGAAGTATGCTTTTTGAAAAGC
TARGET=849,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01118 Ps_contig_mira-and-tgicl-ass_franssen_10368
SEQUENCE=AAGGAATCAAATTTGATATCTAAGACTTGTCTAACACTCACACACACTAGGCAACAACCATTTGTGCTTTGACATTAGAGATTGTGATTTGACACAAAATCAAATACATGATTTTAGTGCTGAATTATCATAATTTTTCTAAGTTATTTTGTTTACTTTTTCAAGACTAGAAATTATTCGAGAATCCAACTTGTCCAAATTCAAATCATAAGTACAGAGTCCACAAGTATTAATTAATTATGATTTATGAAAGG
TARGET=229,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01119 Ps_contig_mira-and-tgicl-ass_franssen_10377
SEQUENCE=ATTTGTTAAGGGATTTGTCTTCATTAGTGCATATGGAAATAACAATTAATTTTARTCAACTAAAAAAAACGAATATTGATATTATTGACTACAGATATTTCACATCAGAAGCAGAATCTTCATACAAACAAAATAAAATCCTCATGTTTTGACTCTTACCAGCAACATAGATAGAGATCCACTCTTCAATGTGCTAAAGGCAGGAATGAAAAGATATGTTATATGATATTACACATCATGACACTTAGCAAATGGAATGGAAGTAACATGTACTTCCAACATGCTTGCTAGCCCATTGCATACTTCTGAGTCCAGCTCCGGGCAGTCGTCTCGTACTTGGACCTGTCTGTCTTGTACATATGAGCAATTTCAGGCACCAAGGGATCGTCTGGGTTTGGATCCGTCAATAGCGAACAAATGGATAGCAATACCTTAGATATTGTCAAAGCGGGACTCCACTGCTCCTTTAAGATATCCAGACAAATACTACCATTGCTGTTGATATTTGGGTGGAAAACTTTAGTCCTAAACGCAACCTTGGGTGGCTTGAATGGATAATCAGGAGGAAAATGAATGGTAACTAGAAAAACACCTCCAGCATAAGGGCTATCAGGGGGACCCATTATAGTTGCTTGCCAATGAAACATATCTTCTGCTACAGGGCCAGCACTGCATGAAGTTGGAGGATCTTTCTGAAGGTCCTTGAGTTCCTTCAGGATCCGCTTGGAGGCCATGGAAATACAGAACAAGATGAGAGAGAGAGAGAAAGAGAGAGAGAGAGAGAGGTAGAACCTAACTTTAAGTTAAAACTAAAGAAAAGAAAAGGTAAGAAAAAGAAAGCACTCGTGTCGTTAGACTACCACTGTCTTCAGACGAC
TARGET=753,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01120 Ps_contig_mira-and-tgicl-ass_franssen_10377
SEQUENCE=ATTTGTTAAGGGATTTGTCTTCATTAGTGCATATGGAAATAACAATTAATTTTARTCAACTAAAAAAAACGAATATTGATATTATTGACTACAGATATTTCACATCAGAAGCAGAATCTTCATACAAACAAAATAAAATCCTCATGTTTTGACTCTTACCAGCAACATAGATAGAGATCCACTCTTCAATGTGCTAAAGGCAGGAATGAAAAGATATGTTATATGATATTACACATCATGACACTTAGCAAATGGAATGGAAGTAACATGTACTTCCAACATGCTTGCTAGCCCATTGCATACTTCTGAGTCCAGCTCCGGGCAGTCGTCTCGTACTTGGACCTGTCTGTCTTGTACATATGAGCAATTTCAGGCACCAAGGGATCGTCTGGGTTTGGATCCGTCAATAGCGAACAAATGGATAGCAATACCTTAGATATTGTCAAAGCGGGACTCCACTGCTCCTTTAAGATATCCAGACAAATACTACCATTGCTGTTGATATTTGGGTGGAAAACTTTAGTCCTAAACGCAACCTTGGGTGGCTTGAATGGATAATCAGGAGGAAAATGAATGGTAACTAGAAAAACACCTCCAGCATAAGGGCTATCAGGGGGACCCATTATAGTTGCTTGCCAATGAAACATATCTTCTGCTACAGGGCCAGCACTGCATGAAGTTGGAGGATCTTTCTGAAGGTCCTTGAGTTCCTTCAGGATCCGCTTGGAGGCCATGGAAATACAGAACAAGATGAGAGAGAGAGAGAAAGAGAGAGAGAGAGAGAGGTAGAACCTAACTTTAAGTTAAAACTAAAGAAAAGAAAAGGTAAGAAAAAGAAAGCACTCGTGTCGTTAGACTACCACTGTCTTCAGACGAC
TARGET=768,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01121 Ps_contig_mira-and-tgicl-ass_franssen_10392
SEQUENCE=GTCGAKGATAAGAGTTCTCTCCATTTCATACTATTGCGGCCGTTGAAGTAAGAGAAAAATAGTCTCACCGAAAGTTTGAGCATCCAAGACACGGTTCTCTCGGCTTTCTCCCAAGGAAGCGAGCTGCTCGTCATCGCGGAAAAGTGAAAGCCTTTCCAAAAGACGATCCTACCAAACCATGCAGCTTGACTGCTTTTGTTGGCTACAAGTCTGGGATGACCCATATAGTTAGGGAGGTTGAGAAGCCTGGATCCAAGCTTCATAAGAAAGAGACATGTGAAGCTGTCACTATCATTGAAAACWCCTCCACTTGTTGTTGTTGGAGTGGTGGG
TARGET=311,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01122 Ps_contig_mira-and-tgicl-ass_franssen_10406
SEQUENCE=GGGGTATTATACAATGTTTAGGGACAGAGTATGATCATCAAGATAATAATAATAAGCTGCATGTTTTCATGGAGTATATGGCTGGAGGTAGTTTGGCTGACGCGTTACACAAGTTTGGTGGTTCGTTCGATGAACATGTTGTTCGTTTGTATACTAGACAGATTTTGCTTGGGTTATATCATCTTCACCAACAAGGAATTGTGCACTGTGACCTCAAATGAA
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.4232
PRIMER_LEFT_PENALTY=2.865899
PRIMER_RIGHT_PENALTY=0.557268
PRIMER_LEFT_SEQUENCE=GGGACAGAGTATGATCATCAAGA
PRIMER_RIGHT_SEQUENCE=GAACCACCAAACTTGTGTAACG
PRIMER_LEFT=20,23
PRIMER_RIGHT=123,22
PRIMER_LEFT_TM=58.134
PRIMER_RIGHT_TM=59.443
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=3.4232
PRIMER_LEFT_1_PENALTY=2.865899
PRIMER_RIGHT_1_PENALTY=0.557268
PRIMER_LEFT_1_SEQUENCE=GGGACAGAGTATGATCATCAAGA
PRIMER_RIGHT_1_SEQUENCE=ACGAACCACCAAACTTGTGTAA
PRIMER_LEFT_1=20,23
PRIMER_RIGHT_1=125,22
PRIMER_LEFT_1_TM=58.134
PRIMER_RIGHT_1_TM=59.443
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=3.4232
PRIMER_LEFT_2_PENALTY=2.865899
PRIMER_RIGHT_2_PENALTY=0.557268
PRIMER_LEFT_2_SEQUENCE=GGGACAGAGTATGATCATCAAGA
PRIMER_RIGHT_2_SEQUENCE=AACGAACCACCAAACTTGTGTA
PRIMER_LEFT_2=20,23
PRIMER_RIGHT_2=126,22
PRIMER_LEFT_2_TM=58.134
PRIMER_RIGHT_2_TM=59.443
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=107
PRIMER_PAIR_PENALTY_3=3.4232
PRIMER_LEFT_3_PENALTY=2.865899
PRIMER_RIGHT_3_PENALTY=0.557268
PRIMER_LEFT_3_SEQUENCE=GGGACAGAGTATGATCATCAAGA
PRIMER_RIGHT_3_SEQUENCE=CGAACCACCAAACTTGTGTAAC
PRIMER_LEFT_3=20,23
PRIMER_RIGHT_3=124,22
PRIMER_LEFT_3_TM=58.134
PRIMER_RIGHT_3_TM=59.443
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=3.4298
PRIMER_LEFT_4_PENALTY=2.872565
PRIMER_RIGHT_4_PENALTY=0.557268
PRIMER_LEFT_4_SEQUENCE=AGGGACAGAGTATGATCATCAAGA
PRIMER_RIGHT_4_SEQUENCE=GAACCACCAAACTTGTGTAACG
PRIMER_LEFT_4=19,24
PRIMER_RIGHT_4=123,22
PRIMER_LEFT_4_TM=59.127
PRIMER_RIGHT_4_TM=59.443
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_01123 Ps_contig_mira-and-tgicl-ass_franssen_10446
SEQUENCE=CAAGTGTCAAATTCTCACCACCCGGAAACAACATAAGCTTTCCTTCACCACCATTTCCACCAACCGAACCACCACCACCGTTCTCCCCGTCCTCTTCATCATCATTCAACTCATCTTCACTCTCACCGCTCCCCTTTTTCTTCTTGTTTTGCATATAACCAATCAACAAAGAAGCCAAAACAACAGCACCTGTCATAAGACTAATAACAATACCAGCAACAGCACCAGAACTCAATGAAGAATTCTTACCACAGCTTTGACAAAGGTGACCACACAATCAGGACTATTCCCTACAAAANAATCCA
TARGET=68,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0612
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.060408
PRIMER_LEFT_SEQUENCE=TAAGCTTTCCTTCACCACCATT
PRIMER_RIGHT_SEQUENCE=GGTGCTGTTGCTGGTATTGTTA
PRIMER_LEFT=33,22
PRIMER_RIGHT=225,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.060
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=193
PRIMER_PAIR_PENALTY_1=0.0705
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.069737
PRIMER_LEFT_1_SEQUENCE=TAAGCTTTCCTTCACCACCATT
PRIMER_RIGHT_1_SEQUENCE=CTGATTGTGTGGTCACCTTTGT
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=280,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.930
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.1089
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.108177
PRIMER_LEFT_2_SEQUENCE=TAAGCTTTCCTTCACCACCATT
PRIMER_RIGHT_2_SEQUENCE=AAACAAGAAGAAAAAGGGGAGC
PRIMER_LEFT_2=33,22
PRIMER_RIGHT_2=148,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.108
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=0.2206
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=TAAGCTTTCCTTCACCACCATT
PRIMER_RIGHT_3_SEQUENCE=TTCATTGAGTTCTGGTGCTGTT
PRIMER_LEFT_3=33,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.3283
PRIMER_LEFT_4_PENALTY=0.267873
PRIMER_RIGHT_4_PENALTY=0.060408
PRIMER_LEFT_4_SEQUENCE=AAGTGTCAAATTCTCACCACCC
PRIMER_RIGHT_4_SEQUENCE=GGTGCTGTTGCTGGTATTGTTA
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=225,22
PRIMER_LEFT_4_TM=60.268
PRIMER_RIGHT_4_TM=60.060
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_01124 Ps_contig_mira-and-tgicl-ass_franssen_10474
SEQUENCE=AGAAACATGAAGAGAAAAAAAAATTAACAAAGACTATAAATGTAGCCTAGCAGGAATTGAACTTGGGACCTAAGCATTACAATCTAAAGCTCTTGCCACTCTACCAGATCTACTTTTCTAACTTTTATAACAGACAGAAAATATAATATAAACAATATGATGAAATAAAAAAAACCAAACAAGAATCATAGTAGCAGTAATGCAGTAGCAGTAGTGCAGTAGTGCAGTAACATGTTTTTTCAAGTTTATCTTCTTCATTCTAACACAATAATGAACTATTTACAAACCACAAAAATATTTCCAAATGCAAAATGATTAATAAATTTTAATATAAAGAATAAAAAATCTAATCTTAACTAAAATTTATAATAATAATAATAATAACATATAATAATTCATAAAACATATTAGAAATAAAATAAATAATAAGATAAAAAGAAAAAGAAAAGAAAAAAATATTAGTTTGAGTTTCTTAACGAAATCAAATCCCAATGCCGGAAAACGAAGAAAACAACAACTTCAAGTC
TARGET=366,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01125 Ps_contig_mira-and-tgicl-ass_franssen_10479
SEQUENCE=GGGGGGGAGKAACAACACGCTGTCGTTTATATTTGAACTCAGTCGAAGAACACATTTTCCTTCTCCCTTTCCCCAATCAGAAAAGCAGTAAGGAACAGAGATGGCAGACGATTTGTTTGAAGGATTGCCTCCTCCTTCTTCCAACACACACTTCCAGCCACCACCACCACCACCGGTTCTTTCCGTTTCCACCATCAACAACACCACCGAATCCTCTACAGTTCCCTCCGTACCAAAGCCAATTCTCAAAAGTTCCCTCAAACGACCTAACCCTACTCAATCCGATAACACCCAAGCTGCAGCACCTAAAAAGAGTTTGAAGTTTAAAACCTCTACGGATGCTTCTGAAGCTCAAGTTATTGACGCTATGAAGAAGATATCTTCGCATATCAAGAACCCTGCCAWMTYCAGCAAG
TARGET=158,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01126 Ps_contig_mira-and-tgicl-ass_franssen_10480
SEQUENCE=TTCTTCATAGCGTCAATAACTTGAGCTTCAGAAGCATCCGTAGAGGTTTTTAAACTTCAAACTCTTTTTTAGGTGCTTGGGTGTTATCGGATTGARTAGGGTTAGGTCGTTTGAGGGAACTTTTGAGAAYTGGCTTTGGTACGGAGGGAACTGTAGAGGATTCGGTGGTGTTGTTGATGGTGGAAACGGAAAGAACCGGTGGTGGTGGTGGTGGCTGGAAGTGTGTGTTGGAAGAAGGAGGAGGCAATCCTTCAAACAAATCGTCTGCCATCTCTGTTCCTTACCTCCCCGCGTTA
TARGET=198,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01127 Ps_contig_mira-and-tgicl-ass_franssen_10490
SEQUENCE=AAGAAATCAGCAAAGGGGTGATGATGTATGATGATGATGATGATGATGATGAGCTATTATTGAATACATTTTCATGTGCTTTTGCTTTCTTTTGTCCTTTCA
TARGET=28,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01128 Ps_contig_mira-and-tgicl-ass_franssen_10491
SEQUENCE=AAGCAGTGGATCAACGCAGAGTCACTTCAATGTCTGCCATGGCACGTGACTGATTAGGGTTAGCAGGGTAGCACGTGCTGGTCTGAGGTGCACATGTGGAGTATTGAGGGAACATGAAGAAATCAGCAAAGGGTGATGATGATGATGATGATGATGATGATGATGAGCTATTATTGAATACATTTCATGTGTCTTTTGTCTTTTCTTTTGTCCTTT
TARGET=134,33
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01129 Ps_contig_mira-and-tgicl-ass_franssen_10492
SEQUENCE=TACTGCTTGTCACTGNCCTAACTAGTGCACATGGGCGATCAACTTTTTTGTAAGTCAACACTTTGCTGCTCCAGAGCAAAGGACAGATCTTTGAGAATCCTCTCGGAATCATCAAAGAAAAGAGAAACAACTTCAAAAACAAACTCAGGGTTATTCTCATCTTGTAGCTGTTGAAGTTGCAGAAACTGACCATCTAAGAACCCCTCCTCGAACATGGTTTTGATGTAGTCCATCCATTGTCTCCGCATCTGACCCACATCCATGTTTCTAAACAAACAAGTCACTTGTTATTGTTATGTCACTAATCACAAAAGAAACAAGTGACCCAGTTGAGTTAAGGTGTTCAAGTTTTGATCTTTAAGAAGAGGGTATACCTTGAAATTGAAGAAGAACCCACGTAGGCTTTCTCTCTCTCTCTCACAAAGTTGTGTATTCTTTCTTTATAATATAGATGTAGTTGTAGTAG
TARGET=406,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01130 Ps_contig_mira-and-tgicl-ass_franssen_10504
SEQUENCE=ATGGCAGACATTTGTTGCGTATGTTAAACGTTAGGTTGTTATTATGGAATTGGCATACCGTTGGGTTCGGTTCTTGGATTCTATTTCAAATTCGGTGCTAAGGGAATATGGTTGGGAATGCTAGCTGGCACAGTTTTGCAAACAATCATTTTATTATACGTCACATTTCGAACTGATTGGAATAAAGAGGTTGTAGAATCAAATAAAAGGCTCAATAAGTGGGAGAACAAGACTGAACCACTACTTAAGAATTAACAAATAAACTCTTAAATCTTCTTGTAACTCGATTAACCCGTTGTAATAAATTCAAATAAGTCAGTTTCATAAATGACAACAAGTAACATATTCTATGTATTAAGATGGTGACCTTCCGTCAAATTTTTAATTTTATGGTATAAATATTTATAAATTAATTAATTTTTTAAAAAGAAAATTAGACAAAAAATT
TARGET=408,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01131 Ps_contig_mira-and-tgicl-ass_franssen_10505
SEQUENCE=AAWTAATGCACAATAATAACTTCGGTCATATCAACAAAAATAWRTAAAAAATAATAAACACTCCTTGCATATCATTCCACAAGAATGAAATTAATTAATTAATTAATTAAATACCTTAATCACTTAAGAAAATCAGGAAGTTCAACATTGTTTACATCCTCAACTTGATTAACCCAACCATACATCTCCATGAAAGGATTAACCAAAGTTTCTGGTGGATAATCATGAACACAATCATCCCAAACATTTCCATATCCATTTCCAAGATCCTTCATCACTTCCCATAAACAACTATTGCATTTAAATCCAGCTCCAAAACTTATCATGAAAATTCTATCACCTTTTTTAATCTCTTTTTTGCTTCCATGTAACCTAACACATACCACAAACTACTAGCAGAAGTGTTACCAAAACGATGAAGTGTCATCCTTGCTGGCTCAAGATCATATTCACTTAGATCTAAACTCATTCCAACTCCATCTATCACTGATTTTCCTCCTGTATGGAGACAAAAATGATCAACACCGGTTTTGAAATTCAATGGTGATTTTTTTGTACTTATTGTTCCTCCACCACTACAAGACTTGGAATAACAACTTATTGTTTTAAGTTTCTCCAAGAGTGACACTAAAAGAAACCTAAATAGTTCTCTTGTTGGTAAAATTTTGGGTGATATTACTCTTAGATTGTCGACAAACGCTCTTGTTGCGGCTTTTGGGAGGAGTTTTTCCTAGGAAAATACCTCTCCTACCTTGCTCATCTTCTTTCTGAATACAACAACCGAAGGACTCGTCTCTCGCGCCGTGGTGAGTTCTTACTAAACACTTCAGTTTGAGTATGGCTTTGTTCTTTAAGGATCTTTTGTTTGTTAGAAGAATCGCGCATCCGCCCGACCGGAAGAGACAGTTGGCAAGGATCATTGATTTGCTGTTTCCGGGATACCAATTTGGACTAAGAGATTCTGATGCGACCAAAAGTGCTAACTTG
TARGET=89,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01132 Ps_contig_mira-and-tgicl-ass_franssen_10507
SEQUENCE=TTGAAATCCGATGTGGGACAAAGTGATGTTATGAAAGGTTCTGGACGCATGCGGCTGCCAATACCAACTGGTAGGGCAATACAGGTGGAAGGTGGCTATGGTGAACGTCTTCCTTCCATTGATACCCGCCCTCCTCGAATGCGTGATTCAGATGCAATCAYKGRGATTATTTTGCAGGGTACTGAAGATGATGAGTCCTCTCGTCGGAATAGATGTCCAAGATCAATCAGATGATGGAGAACCCCAGAGAGAGAGTT
TARGET=246,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01133 Ps_contig_mira-and-tgicl-ass_franssen_10518
SEQUENCE=GTACACCATACAAACCAATATTATTAGTTTATATTCAATAATAACAAAAACATTTTGGTACAATAAGAAGTACAAGTTTTCACTAGATACACTACTTTATGGTGTTTTTACAGTTAATATTTAGATGAACACAACTAATTCCACATCTAATGAGGGTTACAACATCATGTGAGAATCCTAGGTTGAAATGCATTAAATTTAAGACAGCAGATGCTCTCTGTTTGCGTGAACCGGATCAGAGTTATTCTTCTTCTGGTTCTTCTTCTTCCTGTAGAAGATTTTCTCTGTTGTCATGAGCCCAGAAACCACGCACATCTATGAGCATGCTTGCTACAGTTCCTCCCTGTTTGCAGCCTAATAGATCAGCCAATGTTATCTTCAGAGGATCTCCTGGCTTAACCATATCCCAGATTTCATCTCGTACATCCTCAATGCAAAGTTCATAGTTGCCACCTTCAATCCATTTATGGTGGACGTCTCTGAAAAGAGAGTGAATATCCGCTGTAGTAAGGTAACCCCTCCTTGAAGNTCAAGGCAACGAAACAAGTATGTCAAGCCTTCNGGATGTCTTTGTTTA
TARGET=257,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3961
PRIMER_LEFT_PENALTY=0.372557
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=CGTGAACCGGATCAGAGTTATT
PRIMER_RIGHT_SEQUENCE=TAAGCCAGGAGATCCTCTGAAG
PRIMER_LEFT=224,22
PRIMER_RIGHT=397,22
PRIMER_LEFT_TM=60.373
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=0.4275
PRIMER_LEFT_1_PENALTY=0.372557
PRIMER_RIGHT_1_PENALTY=0.054900
PRIMER_LEFT_1_SEQUENCE=CGTGAACCGGATCAGAGTTATT
PRIMER_RIGHT_1_SEQUENCE=ACCATAAATGGATTGAAGGTGG
PRIMER_LEFT_1=224,22
PRIMER_RIGHT_1=470,22
PRIMER_LEFT_1_TM=60.373
PRIMER_RIGHT_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.4757
PRIMER_LEFT_2_PENALTY=0.372557
PRIMER_RIGHT_2_PENALTY=0.103187
PRIMER_LEFT_2_SEQUENCE=CGTGAACCGGATCAGAGTTATT
PRIMER_RIGHT_2_SEQUENCE=GCATTGAGGATGTACGAGATGA
PRIMER_LEFT_2=224,22
PRIMER_RIGHT_2=434,22
PRIMER_LEFT_2_TM=60.373
PRIMER_RIGHT_2_TM=60.103
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.5371
PRIMER_LEFT_3_PENALTY=0.372557
PRIMER_RIGHT_3_PENALTY=0.164560
PRIMER_LEFT_3_SEQUENCE=CGTGAACCGGATCAGAGTTATT
PRIMER_RIGHT_3_SEQUENCE=AAACAGGGAGGAACTGTAGCAA
PRIMER_LEFT_3=224,22
PRIMER_RIGHT_3=348,22
PRIMER_LEFT_3_TM=60.373
PRIMER_RIGHT_3_TM=60.165
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=0.5621
PRIMER_LEFT_4_PENALTY=0.372557
PRIMER_RIGHT_4_PENALTY=0.189529
PRIMER_LEFT_4_SEQUENCE=CGTGAACCGGATCAGAGTTATT
PRIMER_RIGHT_4_SEQUENCE=AACAGGGAGGAACTGTAGCAAG
PRIMER_LEFT_4=224,22
PRIMER_RIGHT_4=347,22
PRIMER_LEFT_4_TM=60.373
PRIMER_RIGHT_4_TM=59.810
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_01134 Ps_contig_mira-and-tgicl-ass_franssen_10550
SEQUENCE=ACGTAAGATAAAATCCCAATACGTTGTATTACAACACCACTGGTCACAGAGATAGTGTCTCCTCCAATTGGGTAGCCCACAACAGTTACTGCATCTTGAAGGGTAGGCAAATCCCCAAACTCCACAGGTGACATACCTTGCCAGAACTCATCATCATCAACGGTAAGCATGGCAATATCACACTCGGTTCCAATGGCAAGAACAGTAGCCAAGTACTTAGTGTCAGAGCCACGTTTCTTGAGCTTGACCTGAGTGTAATGCTCAACAGAATGCGCATTGGTGAGAACCCTCTTCCCACTTA
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0930
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.058853
PRIMER_LEFT_SEQUENCE=CATCTTGAAGGGTAGGCAAATC
PRIMER_RIGHT_SEQUENCE=TACTTGGCTACTGTTCTTGCCA
PRIMER_LEFT=91,22
PRIMER_RIGHT=214,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.941
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=0.1315
PRIMER_LEFT_1_PENALTY=0.072672
PRIMER_RIGHT_1_PENALTY=0.058853
PRIMER_LEFT_1_SEQUENCE=CAACACCACTGGTCACAGAGAT
PRIMER_RIGHT_1_SEQUENCE=TACTTGGCTACTGTTCTTGCCA
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=214,22
PRIMER_LEFT_1_TM=60.073
PRIMER_RIGHT_1_TM=59.941
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.2264
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.192310
PRIMER_LEFT_2_SEQUENCE=CATCTTGAAGGGTAGGCAAATC
PRIMER_RIGHT_2_SEQUENCE=ACTTGGCTACTGTTCTTGCCAT
PRIMER_LEFT_2=91,22
PRIMER_RIGHT_2=213,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=60.192
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.2650
PRIMER_LEFT_3_PENALTY=0.072672
PRIMER_RIGHT_3_PENALTY=0.192310
PRIMER_LEFT_3_SEQUENCE=CAACACCACTGGTCACAGAGAT
PRIMER_RIGHT_3_SEQUENCE=ACTTGGCTACTGTTCTTGCCAT
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=213,22
PRIMER_LEFT_3_TM=60.073
PRIMER_RIGHT_3_TM=60.192
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.3600
PRIMER_LEFT_4_PENALTY=0.301100
PRIMER_RIGHT_4_PENALTY=0.058853
PRIMER_LEFT_4_SEQUENCE=TAGCCCACAACAGTTACTGCAT
PRIMER_RIGHT_4_SEQUENCE=TACTTGGCTACTGTTCTTGCCA
PRIMER_LEFT_4=72,22
PRIMER_RIGHT_4=214,22
PRIMER_LEFT_4_TM=59.699
PRIMER_RIGHT_4_TM=59.941
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_01135 Ps_contig_mira-and-tgicl-ass_franssen_10555
SEQUENCE=AGTGTNTNACGCANAGTACGGTTGATCAATAATATTTCCAATTCTCTTAGAAATATACATATGGCACCTCATGGAGATTCATTACCTAGCTCATTCTCTAGAACCAACAACGTAGTATGCTCCAAACCACCAAAACCTAATTCATCCGATCACAACAACAACAATAACAACAACATTCAAAGAACAATCTCCGACATATCGTTCGCGTTATCAAAAGAAGACTTTGATTTAACACTTCCACCAATAACTGAAGTTGAAGATGCAAAGTGCGAGTGCTGTGGCATGTCGGAAGAGTGTACACCTGAGTACATAAAACGCATACGCGACAAATACTTAGGAAAATGGGTTTGTGGTTTGTGTTCTGAGGCAGTGAAGGAAGAGTTGGAGAAAAATGGAGGGAAAAAAGATGAAGCTTTGAGTGAACACATGAATGCATGTGTTAGGTTTAACAAATATGGAAGGACTTTTCCTGTTTTGTTTCAAGCTGAAGCCATGAAAGAGATGCTGAAAAAGAGTAAAATGGAAGGTAGAAGGGCTAAGTCATTCAACCCTAGAGAGAAAGGTGGTGGTGGTGAAAAGAAAGGTGGACTTGCTAGAAGTTCAAGTTGCATTCCTGCTATCACAAGAGATATCAATGATTTCAAAATTTCAAGTTAAACTTAACTTACAAGTTATTATAACAACTTTTCATTTTACAGGTCAGATAACTTAACTTACACGCATGTTTAGATCAAAAGTGATTATGATAGAATTTCATCATGTTACATTGATTTTAATAAAAATCATGTGTAGATTATCATTTTGTCAAAATCACTATCGTTCTAAAGATGCGGTAAGTCTTTTAATTATGTGTTGATTAA
TARGET=152,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1062
PRIMER_LEFT_PENALTY=0.053515
PRIMER_RIGHT_PENALTY=0.052729
PRIMER_LEFT_SEQUENCE=CCAACAACGTAGTATGCTCCAA
PRIMER_RIGHT_SEQUENCE=AACTCTTCCTTCACTGCCTCAG
PRIMER_LEFT=103,22
PRIMER_RIGHT=382,22
PRIMER_LEFT_TM=60.054
PRIMER_RIGHT_TM=60.053
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.1062
PRIMER_LEFT_1_PENALTY=0.053515
PRIMER_RIGHT_1_PENALTY=0.052729
PRIMER_LEFT_1_SEQUENCE=CAACGTAGTATGCTCCAAACCA
PRIMER_RIGHT_1_SEQUENCE=AACTCTTCCTTCACTGCCTCAG
PRIMER_LEFT_1=107,22
PRIMER_RIGHT_1=382,22
PRIMER_LEFT_1_TM=60.054
PRIMER_RIGHT_1_TM=60.053
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.3451
PRIMER_LEFT_2_PENALTY=0.053515
PRIMER_RIGHT_2_PENALTY=0.291540
PRIMER_LEFT_2_SEQUENCE=CCAACAACGTAGTATGCTCCAA
PRIMER_RIGHT_2_SEQUENCE=CGCGTATGCGTTTTATGTACTC
PRIMER_LEFT_2=103,22
PRIMER_RIGHT_2=324,22
PRIMER_LEFT_2_TM=60.054
PRIMER_RIGHT_2_TM=59.708
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.3451
PRIMER_LEFT_3_PENALTY=0.053515
PRIMER_RIGHT_3_PENALTY=0.291540
PRIMER_LEFT_3_SEQUENCE=CAACGTAGTATGCTCCAAACCA
PRIMER_RIGHT_3_SEQUENCE=CGCGTATGCGTTTTATGTACTC
PRIMER_LEFT_3=107,22
PRIMER_RIGHT_3=324,22
PRIMER_LEFT_3_TM=60.054
PRIMER_RIGHT_3_TM=59.708
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.3451
PRIMER_LEFT_4_PENALTY=0.053515
PRIMER_RIGHT_4_PENALTY=0.291540
PRIMER_LEFT_4_SEQUENCE=CCAACAACGTAGTATGCTCCAA
PRIMER_RIGHT_4_SEQUENCE=TCGCGTATGCGTTTTATGTACT
PRIMER_LEFT_4=103,22
PRIMER_RIGHT_4=325,22
PRIMER_LEFT_4_TM=60.054
PRIMER_RIGHT_4_TM=59.708
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=5.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_01136 Ps_contig_mira-and-tgicl-ass_franssen_10555
SEQUENCE=AGTGTNTNACGCANAGTACGGTTGATCAATAATATTTCCAATTCTCTTAGAAATATACATATGGCACCTCATGGAGATTCATTACCTAGCTCATTCTCTAGAACCAACAACGTAGTATGCTCCAAACCACCAAAACCTAATTCATCCGATCACAACAACAACAATAACAACAACATTCAAAGAACAATCTCCGACATATCGTTCGCGTTATCAAAAGAAGACTTTGATTTAACACTTCCACCAATAACTGAAGTTGAAGATGCAAAGTGCGAGTGCTGTGGCATGTCGGAAGAGTGTACACCTGAGTACATAAAACGCATACGCGACAAATACTTAGGAAAATGGGTTTGTGGTTTGTGTTCTGAGGCAGTGAAGGAAGAGTTGGAGAAAAATGGAGGGAAAAAAGATGAAGCTTTGAGTGAACACATGAATGCATGTGTTAGGTTTAACAAATATGGAAGGACTTTTCCTGTTTTGTTTCAAGCTGAAGCCATGAAAGAGATGCTGAAAAAGAGTAAAATGGAAGGTAGAAGGGCTAAGTCATTCAACCCTAGAGAGAAAGGTGGTGGTGGTGAAAAGAAAGGTGGACTTGCTAGAAGTTCAAGTTGCATTCCTGCTATCACAAGAGATATCAATGATTTCAAAATTTCAAGTTAAACTTAACTTACAAGTTATTATAACAACTTTTCATTTTACAGGTCAGATAACTTAACTTACACGCATGTTTAGATCAAAAGTGATTATGATAGAATTTCATCATGTTACATTGATTTTAATAAAAATCATGTGTAGATTATCATTTTGTCAAAATCACTATCGTTCTAAAGATGCGGTAAGTCTTTTAATTATGTGTTGATTAA
TARGET=562,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2781
PRIMER_LEFT_PENALTY=0.052729
PRIMER_RIGHT_PENALTY=0.225403
PRIMER_LEFT_SEQUENCE=CTGAGGCAGTGAAGGAAGAGTT
PRIMER_RIGHT_SEQUENCE=CTTGTGATAGCAGGAATGCAAC
PRIMER_LEFT=361,22
PRIMER_RIGHT=625,22
PRIMER_LEFT_TM=60.053
PRIMER_RIGHT_TM=59.775
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.2781
PRIMER_LEFT_1_PENALTY=0.052729
PRIMER_RIGHT_1_PENALTY=0.225403
PRIMER_LEFT_1_SEQUENCE=CTGAGGCAGTGAAGGAAGAGTT
PRIMER_RIGHT_1_SEQUENCE=TGTGATAGCAGGAATGCAACTT
PRIMER_LEFT_1=361,22
PRIMER_RIGHT_1=623,22
PRIMER_LEFT_1_TM=60.053
PRIMER_RIGHT_1_TM=59.775
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.5597
PRIMER_LEFT_2_PENALTY=0.334292
PRIMER_RIGHT_2_PENALTY=0.225403
PRIMER_LEFT_2_SEQUENCE=GTTTGTGTTCTGAGGCAGTGAA
PRIMER_RIGHT_2_SEQUENCE=CTTGTGATAGCAGGAATGCAAC
PRIMER_LEFT_2=352,22
PRIMER_RIGHT_2=625,22
PRIMER_LEFT_2_TM=60.334
PRIMER_RIGHT_2_TM=59.775
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=0.5597
PRIMER_LEFT_3_PENALTY=0.334292
PRIMER_RIGHT_3_PENALTY=0.225403
PRIMER_LEFT_3_SEQUENCE=GTTTGTGTTCTGAGGCAGTGAA
PRIMER_RIGHT_3_SEQUENCE=TTGTGATAGCAGGAATGCAACT
PRIMER_LEFT_3=352,22
PRIMER_RIGHT_3=624,22
PRIMER_LEFT_3_TM=60.334
PRIMER_RIGHT_3_TM=59.775
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.5597
PRIMER_LEFT_4_PENALTY=0.334292
PRIMER_RIGHT_4_PENALTY=0.225403
PRIMER_LEFT_4_SEQUENCE=GTTTGTGTTCTGAGGCAGTGAA
PRIMER_RIGHT_4_SEQUENCE=TGTGATAGCAGGAATGCAACTT
PRIMER_LEFT_4=352,22
PRIMER_RIGHT_4=623,22
PRIMER_LEFT_4_TM=60.334
PRIMER_RIGHT_4_TM=59.775
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_01137 Ps_contig_mira-and-tgicl-ass_franssen_10567
SEQUENCE=TTTGAATCGATTTAGTTTAGTTACCAATTTTTGTTAGAMTTTTAGTTAGTTAGTTGTTGTTATGGCATGATTTCTGTTAGGTTATGATTCAGTTAGTTGGTGGGATTAATGAGTGAATATATTAGTAACTGTTATAAGAATACTGTTATGTTATATGTTGAGTTGTTACGACYGAACT
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01138 Ps_contig_mira-and-tgicl-ass_franssen_10573
SEQUENCE=CGAATAGGAGTACATTTATTTATTACTATTAAGACCTTCGTCTTAATTATAACTGTTTAGTTTACGCTTTTGTAATCTACAATCATGCACACATCACAAACAGAATATATATATATATACTACAGAAACAGCATGTTAGGTTATAGCAGAAACACCAAATGAATAACACACAATAAATAACGAATGAATTCGGAGAATAACTTGATCAATGGATTATGTAAAATGGGACAAAAAAGAGGACTCCTCGTGTGTGTCTCGTTTTACATGATCCATTGATCTAGATTCAATCACTCGTTTCACCCACTCACCTTCTTCAATTACCTACTTACGCATCCAATTATGATGAAAAATTGAATCTGAAGCAGTGTTCTTCTGGTAGATCGAGATCTGGTCTTGTTGGTGGTTGATGTGGCGAAGATTCAGAGATCTGAATCGGTTTTGTTTGATAGGTTTAGTTTAGGTTTTCAAGCGCCGATAACTATAACAGAGATCTGAATCGGTTTTGTTTAGGTTTTCACAAGCGCGGATCC
TARGET=105,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1216
PRIMER_LEFT_PENALTY=0.105266
PRIMER_RIGHT_PENALTY=0.016312
PRIMER_LEFT_SEQUENCE=CAATCATGCACACATCACAAAC
PRIMER_RIGHT_SEQUENCE=GAAGAAGGTGAGTGGGTGAAAC
PRIMER_LEFT=79,22
PRIMER_RIGHT=314,22
PRIMER_LEFT_TM=59.895
PRIMER_RIGHT_TM=60.016
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.4031
PRIMER_LEFT_1_PENALTY=0.105266
PRIMER_RIGHT_1_PENALTY=0.297793
PRIMER_LEFT_1_SEQUENCE=CAATCATGCACACATCACAAAC
PRIMER_RIGHT_1_SEQUENCE=GTAAAACGAGACACACACGAGG
PRIMER_LEFT_1=79,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=59.895
PRIMER_RIGHT_1_TM=59.702
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.5209
PRIMER_LEFT_2_PENALTY=0.504596
PRIMER_RIGHT_2_PENALTY=0.016312
PRIMER_LEFT_2_SEQUENCE=ATCATGCACACATCACAAACAG
PRIMER_RIGHT_2_SEQUENCE=GAAGAAGGTGAGTGGGTGAAAC
PRIMER_LEFT_2=81,22
PRIMER_RIGHT_2=314,22
PRIMER_LEFT_2_TM=59.495
PRIMER_RIGHT_2_TM=60.016
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=234
PRIMER_PAIR_PENALTY_3=0.6348
PRIMER_LEFT_3_PENALTY=0.618494
PRIMER_RIGHT_3_PENALTY=0.016312
PRIMER_LEFT_3_SEQUENCE=CATGCACACATCACAAACAGAA
PRIMER_RIGHT_3_SEQUENCE=GAAGAAGGTGAGTGGGTGAAAC
PRIMER_LEFT_3=83,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=60.618
PRIMER_RIGHT_3_TM=60.016
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=232
PRIMER_PAIR_PENALTY_4=0.8024
PRIMER_LEFT_4_PENALTY=0.504596
PRIMER_RIGHT_4_PENALTY=0.297793
PRIMER_LEFT_4_SEQUENCE=ATCATGCACACATCACAAACAG
PRIMER_RIGHT_4_SEQUENCE=GTAAAACGAGACACACACGAGG
PRIMER_LEFT_4=81,22
PRIMER_RIGHT_4=263,22
PRIMER_LEFT_4_TM=59.495
PRIMER_RIGHT_4_TM=59.702
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_01139 Ps_contig_mira-and-tgicl-ass_franssen_10575
SEQUENCE=GTCKCTCTGTTATATCGTTCTGTTGTCTCTTCTTCTCTTCTTTCTCTCTCTTCTTCTCTGAAACTAAGTTCCAGCTTGGAAGGGTCCACCACTCCACGGTGGAGTTTCTGGATCTCACCGTCGCTCTTTCAGCTTTCTCCCTTAACTTTGACACAAAGTAAAACATTCTTTTCTTCGATAAGTTCTTTCGGCTTGATTTTGATTCTGATTCTTGTTCTTAGTCAAGAGGAATTTTAGGGTTTTGTGGTAGAGAAAGAAAGGGGATTACGACGTCGGTTTAGGATGCCGGGACGTCGGTCTAAGTCGGAGAAGAAAGATGACGACGAGAAGCAGCTCCGGCGAGACCCGTATGAAGTCCTTGGCGTTAATCGGAACTCCACCGATCAGGAAATTAAATCCGCTTACCGGAAAATGGCTCTCAAGTTTCATCCTGACAAGAATGCTAATGATCCCAAAGCAGCTGATATGTTCAAAGAGGCTACCTTTTCTTATAATATCTTAGCGGATCCTGATAAACGGCGCCAGTATGACTCATCTGGCTTTGAGGCCGTTGAATCAGATAACCAGGAGTTAGAGTTGGATCTTTCAAGTTTGGGTGCTGTCAACACAATGTTTGCTGCACTTTTTAGCAAACTTGGTGTACCAATCAAGACAACTGTATCTGCGACTGTTTTGGAAGAGGCACTCAATGGGTTAGTTACAATTCGCCCACTTCCGTTGGGAGAGTTCATATCTAGAAGGGTTGAGAAGCAGTGTGCACATTTTTATTCAGTTACAATAACAGAAGAGGAAGCAAGAGCTGGATTTGTTTGTCGAGTACAATCATCAGACAAAAGCAAGTTCAAGTTATTATACTTCGACCAGGAAGACAATGGTGGCTTCAGTCTTGCCCTCCAGGAAGACAGTGCAAAAAATGGAAAGTTTACTTCTGCTGGAATGTATTTTCTTGGCTTCCCTGTTTATCGGCTAGATCAAACAATGAACTCCATAGCTGCGGCCAAGGATCCAGATACTTCATTTTTCAAAAAGCTTGATGGGTTCCAGCCCTGTGAATTAACTGAATTGAAGGCTGGTACCCATACATTTGCGGTTTATGGTGACAACTTTTTCAAAAGTGCAAACTATACAATAGAAGTCCTGTGTGCTGCACCTTTTAATGAAGAAAAAGAAAATTTAAGAAATGTAGAAGCTCAAATTTTGTCCAAGAGAGCAGAAATATCAAAGTTTGAATCAGAATATCGAGAGGTTCTTGCACAATTCACAGAAATGACAAGCAGATATGCACATGAAATGCAAACGATTGACGAGCTATTGAAACAAAGAAATGAGATACATGCCTCGTACACAGTTGTTCCTCTAAAACGTAGTAGCAGCAGCAGCAAGGGCAGAAGTAAAACATCTTCAAAGGAGTCAAAAGAAGATGGTGAAGTAAGAGAAAAGAGGAGTACAAGAGAACGGCCAAGGAAGAAAAAATGGTATAACCTCCACTTAAGAGTTGACAAGAGAAAGGCTTGCTAAGGTGAGAGAATTGGAGAACTAAGAAATTCTTAATTCTTTTGCTGTCACTCATCATGTATTATTAAGTAACACATTATTTTATAGTAGCTTGTCAACTCTGAATGCCATTATCAAACGCTATAGTCGGTTGATCTTTTGATTATATAGCTCTTGTACAAGCTATAAGAGGAAGGGTTTGGCAAAGCAACAAAACAACTCAATGTAGTTGGAAGGTTTTATTTATGTTTGCCATTCAAGGATGACAACAACGCATACTTCTTATTGATTTTGTATAATCCCTTTCCCCTCTTTCCTTTTATTTTGTCTCTTAGTAGCTCTCATCTTCAGGATTCGAATTTGTAAATTGTTGATTTATTGATGAAGAATGGTAAATTCTCTAAGCGGGTTGTAATTTTATTTTGAAATATTGGCAAAGCGTTGGAAGATAGAGCCTGGTAATTTCTTGTTCAATATTACCAAATAATAATCTCAAATGTTGGCTAAATATGGGGCCGTTATTATTTCTCTACAYTCTATAATT
TARGET=1369,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01140 Ps_contig_mira-and-tgicl-ass_franssen_10583
SEQUENCE=GTATAGAGATAAGAATAAATAGAATAGCAGAGATAAGAAGAAATTAGGATGAGTTCTGTTCAGCTATGGAACACGACCACCACAACAACCACCACCACTACTAATAACAATGATAATAATAATAATGCTTATGAATCTGATCATAAAGTTCGGAACAATCAAAATAAATTAGAAACCAAGAAAAGGGAAGAGGAAGAAAAATCTGTGACGGTAGCTGAAGATCGTTTTCAATCTTGTAGTAGTAATAGGAATGGAGGAAGTGCTTTTATGCCATTTTCTACGTATTCTTCTGTTCCTGTGACGACGGTTACATTGGCTGCACCTAAAGATGAGAAAGATGAAAGTGGTGTGAATAGACTGTCTTTTATGATGCCTGGAGTGAAAGGTTTGAGGGAAGGATTTGATTCGAGGGGATCGAGGAGTAGTTCTAGCAGGGCTGTTTCTGTTTCGTCTTCTCCTCCTACGGTGCAGCCGAGTTTGCGCAGTGCGCCAGTTCAGCCGCAGCAGATTGCTAGGAAACAGAGGAGGTGCTGGTCGCCGGAGTTGCATCGGAGATTTGTTAATGCATTGCAGAAGCTTGGAGGCTCTCAAGCAACGCCAAAGCAAATCAGAGAACTGATGCAAGTTGATGGCTTAACTAATGATGAAGTGAAGAGCCATTTGCAAAAATACCGGCTTCATACACGGAGAGTTCCAGCTGCAAACGGTAACGAGTCTGTTGTAGTTCT
TARGET=113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2527
PRIMER_LEFT_PENALTY=0.150726
PRIMER_RIGHT_PENALTY=0.102004
PRIMER_LEFT_SEQUENCE=CACAACAACCACCACCACTACT
PRIMER_RIGHT_SEQUENCE=CTTCCTCTTCCCTTTTCTTGGT
PRIMER_LEFT=80,22
PRIMER_RIGHT=195,22
PRIMER_LEFT_TM=59.849
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=0.2602
PRIMER_LEFT_1_PENALTY=0.150726
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=CACAACAACCACCACCACTACT
PRIMER_RIGHT_1_SEQUENCE=CCGTCACAGATTTTTCTTCCTC
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=210,22
PRIMER_LEFT_1_TM=59.849
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.2991
PRIMER_LEFT_2_PENALTY=0.197140
PRIMER_RIGHT_2_PENALTY=0.102004
PRIMER_LEFT_2_SEQUENCE=CTGTTCAGCTATGGAACACGAC
PRIMER_RIGHT_2_SEQUENCE=CTTCCTCTTCCCTTTTCTTGGT
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=195,22
PRIMER_LEFT_2_TM=59.803
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=141
PRIMER_PAIR_PENALTY_3=0.3066
PRIMER_LEFT_3_PENALTY=0.197140
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=CTGTTCAGCTATGGAACACGAC
PRIMER_RIGHT_3_SEQUENCE=CCGTCACAGATTTTTCTTCCTC
PRIMER_LEFT_3=55,22
PRIMER_RIGHT_3=210,22
PRIMER_LEFT_3_TM=59.803
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.3616
PRIMER_LEFT_4_PENALTY=0.150726
PRIMER_RIGHT_4_PENALTY=0.210886
PRIMER_LEFT_4_SEQUENCE=CACAACAACCACCACCACTACT
PRIMER_RIGHT_4_SEQUENCE=AAAACGATCTTCAGCTACCGTC
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=59.849
PRIMER_RIGHT_4_TM=59.789
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_01141 Ps_contig_mira-and-tgicl-ass_franssen_10589
SEQUENCE=GATGTGCTTAAAGAAGAAGGGTATGGAGGAGAAGGTAGCAGATGGAAGAATGTTGGTGTCACTGTWGGCTTCATCATGGTTTACAGATTCATTTCCTATGTTATTCTGAGATACAAATGCTCAGAGAGGAGGGTCTAGAGCCTATGGTGAGTCTCTCTCTCTCTCAATCACTTTCTCTCTCTATGGAGTTCACATGTTCTTGGTATTTTACTTATGATGGCAACCATAGAAGATAATATCAAACCAAAAAAAATAGTAAAGAATTAG
TARGET=152,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01142 Ps_contig_mira-and-tgicl-ass_franssen_10594
SEQUENCE=TAGAAAATCCTACCTTAAAGATTGAAGAAATGATTGGTTATATAGGAGGGGAGTGATGCTAACAGAAGTGGGGTAGTTAGGTCAGGAGGCGGTTAGGGCAATGCACCTCTGTCTAGTTTTTCAGTTGGTTTTGGTTGTTAGCAGTTAGCAATTCTGTTAGCCTTTGTTAACCTTTGGTTTTGAATTTAGTTACGATGTTAGAGTCTAAGTTAGTGTTAATTTCACTTAGTTAGTTAGGAAAGAGTTAAAAACTGTTTTAATTGTTAGTTATGATATCAATTAGTGAATGAGTAGTACTGTTAAGTTGAGTTAGCTCAATTAGAAGGTTAGTGAAATGAAATGAAGTTGGACTGTCATAGCTTGAATGGATGGTGTGGTATTGSTTTAGTTAGTTATGGCTAAATCTGTTATGTTATTTTAGTCTAGAKTTAGGTGTTAATAATTAAGATTCAAGCTAGTTAGTAGCTAGTTGTAGTTTTATTAACAAGTTAGCTTGTTAGTTGAAATTAGAGTTATGTTGRCAGTTAGTAAAGTTAGGCTGGATAATTGTGATGTTAGTTTAGCAAGCCAACTATAACTCTTATGAATTTACCAAAGGTTTGAATC
TARGET=226,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01143 Ps_contig_mira-and-tgicl-ass_franssen_10596
SEQUENCE=TGTTTAGAATGGATTGCCGACGCCGAAAGGGAAACTATGTTCGTTTTTCTGAGGAGGAGGAGGAGGAAGAGGGAAAATCTGATATGTGATTTCATGGATGAGGATGGTTTTTAGTTTTATGCATTGTTTTGTTTTGTTTTGAAGGCTATGAATGGCCACAGTTTTTGTGGTCGTCAATTTTATAATTCCAGAACTAAATAACATTGTTGCTCCAATTATAGTGTTTTGTGGAACTGGTGTAAAAAACACTTGACAGTTGCATGCAGCTTTTGCTTA
TARGET=51,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5246
PRIMER_LEFT_PENALTY=0.460533
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=CGAAAGGGAAACTATGTTCGTT
PRIMER_RIGHT_SEQUENCE=ATTGACGACCACAAAAACTGTG
PRIMER_LEFT=23,22
PRIMER_RIGHT=177,22
PRIMER_LEFT_TM=59.539
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.6792
PRIMER_LEFT_1_PENALTY=0.460533
PRIMER_RIGHT_1_PENALTY=0.218662
PRIMER_LEFT_1_SEQUENCE=CGAAAGGGAAACTATGTTCGTT
PRIMER_RIGHT_1_SEQUENCE=GCATGCAACTGTCAAGTGTTTT
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=59.539
PRIMER_RIGHT_1_TM=60.219
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.9468
PRIMER_LEFT_2_PENALTY=0.460533
PRIMER_RIGHT_2_PENALTY=0.486257
PRIMER_LEFT_2_SEQUENCE=CGAAAGGGAAACTATGTTCGTT
PRIMER_RIGHT_2_SEQUENCE=CAAAAACTGTGGCCATTCATAG
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=166,22
PRIMER_LEFT_2_TM=59.539
PRIMER_RIGHT_2_TM=59.514
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=1.0719
PRIMER_LEFT_3_PENALTY=1.047725
PRIMER_RIGHT_3_PENALTY=0.024206
PRIMER_LEFT_3_SEQUENCE=TGTTTAGAATGGATTGCCGAC
PRIMER_RIGHT_3_SEQUENCE=AACCATCCTCATCCATGAAATC
PRIMER_LEFT_3=0,21
PRIMER_RIGHT_3=108,22
PRIMER_LEFT_3_TM=59.952
PRIMER_RIGHT_3_TM=60.024
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=1.1118
PRIMER_LEFT_4_PENALTY=1.047725
PRIMER_RIGHT_4_PENALTY=0.064105
PRIMER_LEFT_4_SEQUENCE=TGTTTAGAATGGATTGCCGAC
PRIMER_RIGHT_4_SEQUENCE=ATTGACGACCACAAAAACTGTG
PRIMER_LEFT_4=0,21
PRIMER_RIGHT_4=177,22
PRIMER_LEFT_4_TM=59.952
PRIMER_RIGHT_4_TM=59.936
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_01144 Ps_contig_mira-and-tgicl-ass_franssen_10596
SEQUENCE=TGTTTAGAATGGATTGCCGACGCCGAAAGGGAAACTATGTTCGTTTTTCTGAGGAGGAGGAGGAGGAAGAGGGAAAATCTGATATGTGATTTCATGGATGAGGATGGTTTTTAGTTTTATGCATTGTTTTGTTTTGTTTTGAAGGCTATGAATGGCCACAGTTTTTGTGGTCGTCAATTTTATAATTCCAGAACTAAATAACATTGTTGCTCCAATTATAGTGTTTTGTGGAACTGGTGTAAAAAACACTTGACAGTTGCATGCAGCTTTTGCTTA
TARGET=124,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1736
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=ATGTTCGTTTTTCTGAGGAGGA
PRIMER_RIGHT_SEQUENCE=ATTGACGACCACAAAAACTGTG
PRIMER_LEFT=36,22
PRIMER_RIGHT=177,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=142
PRIMER_PAIR_PENALTY_1=0.2429
PRIMER_LEFT_1_PENALTY=0.024206
PRIMER_RIGHT_1_PENALTY=0.218662
PRIMER_LEFT_1_SEQUENCE=GATTTCATGGATGAGGATGGTT
PRIMER_RIGHT_1_SEQUENCE=GCATGCAACTGTCAAGTGTTTT
PRIMER_LEFT_1=87,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=60.024
PRIMER_RIGHT_1_TM=60.219
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.3282
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.218662
PRIMER_LEFT_2_SEQUENCE=ATGTTCGTTTTTCTGAGGAGGA
PRIMER_RIGHT_2_SEQUENCE=GCATGCAACTGTCAAGTGTTTT
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=263,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.219
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=228
PRIMER_PAIR_PENALTY_3=0.5246
PRIMER_LEFT_3_PENALTY=0.460533
PRIMER_RIGHT_3_PENALTY=0.064105
PRIMER_LEFT_3_SEQUENCE=CGAAAGGGAAACTATGTTCGTT
PRIMER_RIGHT_3_SEQUENCE=ATTGACGACCACAAAAACTGTG
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=177,22
PRIMER_LEFT_3_TM=59.539
PRIMER_RIGHT_3_TM=59.936
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=0.5958
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.486257
PRIMER_LEFT_4_SEQUENCE=ATGTTCGTTTTTCTGAGGAGGA
PRIMER_RIGHT_4_SEQUENCE=CAAAAACTGTGGCCATTCATAG
PRIMER_LEFT_4=36,22
PRIMER_RIGHT_4=166,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.514
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=131
=
PRIMER_SEQUENCE_ID=SSR_01145 Ps_contig_mira-and-tgicl-ass_franssen_10597
SEQUENCE=GAGTTCGSGGGATTCATTCTTTTGTTCTCTAATCATTCATTTCAATCTTTTTTCTCTCTGCAAAATTCCCACAAACCTTTCTACTTTTCCTTTTCAATGGAGACCCCATCATCCACAACCAGGTTCACCAACACCATTCCACTCTCAAGAAAATTCGAAGATTCTGAAAAGACCATGTCGAAATCAAAGGCACAACAGCAGCAAGATCGATGTGCATTGATGGACATGACAAACGATTCACCGATCGTTGGGCTCGCCAACGGTGGCAATCTTGACACACCATCGGGGAAGCAAAAGGCTAGCCGAGTGAAGAACACTCCGGGATCCGGCGAGGCCTTGCTGAGAGGTCAAGCAAAGACTCTTATGCAGATGGTGGAAGAAGAGGCTTTGGATGTTTCTGGAAATTCCACTGATGGATTGCCTTCAATCACCTCTTCTGCTGTTCATCAGGTGGTGAATATGGAGGAAGATGAAAAGTATGAGATAGCAAAATCCTTGGATTTCTCGGAAAAATCTCAAGTATCAGAAGAATGTAACTCTGAAGTGAGTTATCAGGAAGTAACACAAGGAAGCTGTGTTGGAAGCTGCAGTGTTGAGGATGATGCTTCCGTCTGGTCAATGCAAGTGAGTGCGAGCAACAAGGATGAAGATGATGTGGAGGAAGAAATAGCTGAGGAAGAAGATGATGAAGAAGACTATTATGATGTGGATGATGAAGAAGAATATGAAGAGGATTTGGGGCTTGATGAACTGTGTGAAGGATTAAACAAGGTTAATGTGAATGAAAAGGTGGTTTCGAAATTTGCTGGGAAACATACAAGGTTTGTTTACAACAGTGATGATGAAATTGTGAAAGAAGAGGAAGATGAAAGTGTTGATGACAGTAATGTGTTGCGTTTGAATGGATTGCCGACGCCGAAAGGGAAACATGTTCGTTTTTCTGAGGAGGAGGAGGAGGAGGAAGAGGGAAAATCTGATATGTGATTTCATGGATGAGGATGGTTTTTAGTTTTATGCATTGTTTTGTTTTGTTTTGAAGGCTATGAATGGCCACAGTTTTTGTGGTCGTCAATTTTATAATTCCAGAACTAAATAACATTGTTGCTCCAATTATAGTGTTTTGTGGAACTGGTGTAAAACACTTGACAGTTGCATGCAGCTTTTGCTTACTCGACATATATGCTGACAGGCTATGATTG
TARGET=943,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01146 Ps_contig_mira-and-tgicl-ass_franssen_10597
SEQUENCE=GAGTTCGSGGGATTCATTCTTTTGTTCTCTAATCATTCATTTCAATCTTTTTTCTCTCTGCAAAATTCCCACAAACCTTTCTACTTTTCCTTTTCAATGGAGACCCCATCATCCACAACCAGGTTCACCAACACCATTCCACTCTCAAGAAAATTCGAAGATTCTGAAAAGACCATGTCGAAATCAAAGGCACAACAGCAGCAAGATCGATGTGCATTGATGGACATGACAAACGATTCACCGATCGTTGGGCTCGCCAACGGTGGCAATCTTGACACACCATCGGGGAAGCAAAAGGCTAGCCGAGTGAAGAACACTCCGGGATCCGGCGAGGCCTTGCTGAGAGGTCAAGCAAAGACTCTTATGCAGATGGTGGAAGAAGAGGCTTTGGATGTTTCTGGAAATTCCACTGATGGATTGCCTTCAATCACCTCTTCTGCTGTTCATCAGGTGGTGAATATGGAGGAAGATGAAAAGTATGAGATAGCAAAATCCTTGGATTTCTCGGAAAAATCTCAAGTATCAGAAGAATGTAACTCTGAAGTGAGTTATCAGGAAGTAACACAAGGAAGCTGTGTTGGAAGCTGCAGTGTTGAGGATGATGCTTCCGTCTGGTCAATGCAAGTGAGTGCGAGCAACAAGGATGAAGATGATGTGGAGGAAGAAATAGCTGAGGAAGAAGATGATGAAGAAGACTATTATGATGTGGATGATGAAGAAGAATATGAAGAGGATTTGGGGCTTGATGAACTGTGTGAAGGATTAAACAAGGTTAATGTGAATGAAAAGGTGGTTTCGAAATTTGCTGGGAAACATACAAGGTTTGTTTACAACAGTGATGATGAAATTGTGAAAGAAGAGGAAGATGAAAGTGTTGATGACAGTAATGTGTTGCGTTTGAATGGATTGCCGACGCCGAAAGGGAAACATGTTCGTTTTTCTGAGGAGGAGGAGGAGGAGGAAGAGGGAAAATCTGATATGTGATTTCATGGATGAGGATGGTTTTTAGTTTTATGCATTGTTTTGTTTTGTTTTGAAGGCTATGAATGGCCACAGTTTTTGTGGTCGTCAATTTTATAATTCCAGAACTAAATAACATTGTTGCTCCAATTATAGTGTTTTGTGGAACTGGTGTAAAACACTTGACAGTTGCATGCAGCTTTTGCTTACTCGACATATATGCTGACAGGCTATGATTG
TARGET=1019,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01147 Ps_contig_mira-and-tgicl-ass_franssen_10623
SEQUENCE=GAGATAATAATAACTCTCTTKAGTCTTTACTTTCATTGCAATCTAGTTCTATAGTCTCTCTCTTTGGAAAAGGAAAGAGAAAAAGTAGTGAAGAGAAGAGAAGAAACATAGCAAGTACAAGGTCATTGAAAATGGGTCGTGGGAAGATAGAGATAAAGTTGATTGAGAATCCAACAAACAGGCAAGTGACATACTCAAAGAGAAGGAATGGTATATTCAAGAAAGCTCATGAGCTTAGTGTTCTTTGTGATGCTAAGGTCTCACTCATCATGTTCTACCAAAAACAACAAGATG
TARGET=90,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01148 Ps_contig_mira-and-tgicl-ass_franssen_10630
SEQUENCE=CTTTCGAGGTTGCTGCTCACAGCTTGCTTCTGTTATGGGGTCACAACCACAGTGTCTATGCGAGGTTGTTAACGGCGGTGCTGCTTCATCTATTGCTGCTAGTTTTAACATCAATCAAACTCGCGCTTTAGCACTTCCAGCAACTTGTAATGTCCAAACACCTCCTATCAACACTTGCACCGGTTCAGCTTCTTCTTCTTCTCCTCCTGTGGGAATTTCTGTTTCCAACATTCCGAATTCTTCTTCAGGGAGTTTTTCATCAACTACTGGAAGTAGTGAAAGTATTCGTGGCTCATCGTCGTCTTATAGAACATCATCAAGTTCTGGAAAATTACAATGTTCTTTGCTTGTTCTGGTTATCTTTGCAAAGTTCACATTCATATTGATGACCACTACTTAGGTTGCGTGTGCATGTTTTTGTAAACCTTATTAGTATTAGTCTAAATTCTACTTATTTTAGGCTACCCTTTGTTTTTCTCCCTTGCACTATTAACGAAGACATAATCATGTAAAAGTGCTATATTATTTTGNGAAATGACTATTAAGGAGAAAATTTCTT
TARGET=189,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0872
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.054900
PRIMER_LEFT_SEQUENCE=CCAGCAACTTGTAATGTCCAAA
PRIMER_RIGHT_SEQUENCE=GAAGAAGAATTCGGAATGTTGG
PRIMER_LEFT=136,22
PRIMER_RIGHT=245,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=59.945
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=0.1055
PRIMER_LEFT_1_PENALTY=0.081990
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=GCTTTAGCACTTCCAGCAACTT
PRIMER_RIGHT_1_SEQUENCE=GAAACAGAAATTCCCACAGGAG
PRIMER_LEFT_1=124,22
PRIMER_RIGHT_1=224,22
PRIMER_LEFT_1_TM=60.082
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.1369
PRIMER_LEFT_2_PENALTY=0.081990
PRIMER_RIGHT_2_PENALTY=0.054900
PRIMER_LEFT_2_SEQUENCE=GCTTTAGCACTTCCAGCAACTT
PRIMER_RIGHT_2_SEQUENCE=GAAGAAGAATTCGGAATGTTGG
PRIMER_LEFT_2=124,22
PRIMER_RIGHT_2=245,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=59.945
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=0.1652
PRIMER_LEFT_3_PENALTY=0.032347
PRIMER_RIGHT_3_PENALTY=0.132857
PRIMER_LEFT_3_SEQUENCE=CCAGCAACTTGTAATGTCCAAA
PRIMER_RIGHT_3_SEQUENCE=TCTATAAGACGACGATGAGCCA
PRIMER_LEFT_3=136,22
PRIMER_RIGHT_3=309,22
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=59.867
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.2148
PRIMER_LEFT_4_PENALTY=0.081990
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_LEFT_4_SEQUENCE=GCTTTAGCACTTCCAGCAACTT
PRIMER_RIGHT_4_SEQUENCE=TCTATAAGACGACGATGAGCCA
PRIMER_LEFT_4=124,22
PRIMER_RIGHT_4=309,22
PRIMER_LEFT_4_TM=60.082
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_01149 Ps_contig_mira-and-tgicl-ass_franssen_10639
SEQUENCE=CTTTAGCAAAAAGGAGCCGAATTCCAATTAAAGGAAAATGCAATATAAGATACACAAGTCACATAACATTCATATAGATTAGTCTTTTCCATCTACCATGATTTATTGAAAACATAACATTCAATTATTTACTACCACAAAATGAAGTGTTTTTCCCCCAACTTGAAAGATTCAATGCATTCATTCTACTTCAAAACCCTTCTCCTGAAGCTCAGCAATAATTTCATTCCTCATCCTCAACCAAAGCTTCTGAGCCTCCTGCTCAAACTTCTTATCCTTAAGCTCCTTCTTATAATCACTTTCATCCATATCATTCTCCACCATTCCTTTAGGTCCAATAGTTGTTCCAATCATTCCACCTTGTTTAACAAACTCCTCCATAGCTTCTGCGTCACCGGGATACGGAAACTCACGCCGCTCATACAAATGAGCCATATCCTTCTCTTCCTTTGGCATTGGCTTCAGCGTCCACCAACCCAATGGCGATGTCTCGTTGTATAACCACGCTGCACCCATCACTGTATAGGTGCATATCAAAGCCTTCCCAATTTGTTTCCAGAAGTAGTAGTTATTTGGCCCTAGTCCTATTTTCTTCAGATAGTATTGGGCATGTTGTATAGCTGTTTGGGGTTCCTGGATTCCGAAGCGTTTACGAGGCATCGAGATTGAGAAGTGAGCTACGATGAATATGAATGAATGATTATGCTGTTTTTATTTCATATATTCATC
TARGET=689,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01150 Ps_contig_mira-and-tgicl-ass_franssen_10640
SEQUENCE=ATAAAACGACATATTTTAATTTGTAATTTAATCTAACTATTACACCAACAATGACATCAATTCATACTCATAGAAAGTAACTTTAAGAAACATGAAAACATTCTTATATCAAGACAACATCAAATTTGCACTGAATAAACAACTAACAAACAAACAATATGAAAAACATATTCAACTCTTAAAGCATAGCCAATTGTTGAATATTAATAGGAGAAGTATAAGATTGTGAAGTATATGATTCTTTTGCTAGTAAAATATTAGCCATCTCAGTAGGGTGAAAAGCATCCCAAAACAAATACTTGCCACGTTCTTCACATGGTTGTTGCAATGGAAGACAAGTGATTTGTCCATTGTTTTTACCAACACCACAGCATCCTTTGTCTACAACATCAAATCCATATGATTTTCCATTTGCAGCTAGATCTTCACTACTTTGATAAAAATCCAAGTACACAAACTTTGATCCTGGAAGCTGTCCTCCATTGAAATTCTGAACTAACTGTTTAAGACCTGAGTTGAAATATATAATCGCGTCGTTGATCTTGTCGTTGCATCTGCTGTTGTTTCCATTGAAACGCGCTAATTCGTATGGTATGCATCCGATTTGTCCAACTGCTGCAACAATCACTTTTCTTGCACCTAATGAATGTAATTGAGTTAACTGACGAGCGTAAGCTTGAAGAAGGGCAGAAGCAAAAGCTTTAGGAGTGTATTGAGCGTTAGTCGAAATAAAAATCCGTCATGAAATAGTTATTAAGGTAATCATTGCTTGCCATTCCTGAAT
TARGET=145,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01151 Ps_contig_mira-and-tgicl-ass_franssen_10647
SEQUENCE=CCCTTCTTGTTATTGTAACTACTTCAAAGAAATTATCATGCAAGAATTGCATATWATAAGCAACAAGAGAAACTTWCAAAATATAACTTGGTTCCTACCTAGCAAATAGGAAATAAAAAACAACCAAGTGCCTCCACTTCCTATTTTTTTCCCCTTCTTCTGTGAGCCTTTCCCTTTAGTATTGTCCGTGGAAGAATCCATATTAGTATCCTTGATATTAATGGCACCCTGGCTGAATTCCGGAGACAAAATCTTAGCAATCTCAGGAAAACCAACACTGTTTCTGCCACGCAACAGTGTTTGCAAATTTTGCTCCCAAAGTTTGTCTTTCTTAATACCAGGACTCCTGAAACTGTAGAGTTTGATGAACTTTGAAATGATGGAGGCGATTTCGGAACGAGAGTGATGAGGGATGCCCATGAAGGTGCAGAGCTCACTGGTAGCTTTTCGGTTCTTGGTTTTGCCGACGCCTACAATTTCAGCGACGGCTTTAGATACCGTCGACATGCTCCTCCTCCCTGATGCTGCACCCATCGGATTCATTGTTTCGGCTGGACTCTTCTCTTCTCTTTTCTCTTCCAATTTGATTTATCTGTGTTGCCGTTGATCCCACCCAACTTCCGAGCSA
TARGET=557,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01152 Ps_contig_mira-and-tgicl-ass_franssen_10648
SEQUENCE=GAACTCGAGTAGCACGTGTGACAGTTAATACAAAGAGTAGCAAGCGCCGCAGTGCGTAATCACAAGGAAGACCGTTCGATCGAACACCACGTCATCGATCCGCGTGAAATCGTATCTGAACGGCTCAGATCATTTTCACAGAGTAACTGAAGAAACGRAGCGAAGAAGAGTCTGGGAACTTGGCTTCGTTGTTTCTCTACTCTCTCGTGCTCGATTTTCCACTCTTCAATGAAAACAGAATCTCAAATTTTTGTGGCGTCGAAGATGAATTTCACGGAGGATTCTGAGAAAGTGAAGAAAATTGAGGTTGTTGTTTTGAGTGAAGATGAAGGTGAAGACGATTATGAGTCAGTGGAAGAAGTAGACGCCGGAGAAGACGACGGTGATGAGGATGACGATGTTGATGATGAGGAGGAGGATGAAGAGGAGGAGGAGGAGGATGCTCCTGACGCCGGCAACGACGAGGATGATGATGACGAAGAGGAAGATGGAGATGACGAAGCAGGTGGTGGTGAGCGTGGTGGTGACTCTGATGATGATGACGACGACGACGAGAACGATGACGACGAGGAGGATAACGATGAAGAGGATGATTTGGGAACAGAGCACCTCGTTCGCCCTATAGGCAATGCTGAAGAGGAGGAAGCTTCTAGTGATTTTGACCCAGAAGAAAATGACGAGGATGAAGACGAAGATGATGATGAGAAGGATAAGGTTCCACCGAAAAGGAAGAGGTCAAATAAAGATGATGATTCCGATGACGATGATGGAGGTGAAGATGATGAAAGACCCTCGAAAAAATAGAGTTGGGCTATTTCTTTGTTTGAGGACTTAACAAACCAAACCATAGTTGAAGTAGATCAGCATCTGTCCTTTGAACGCTTTTCAGAGAAAGGATGAGTAGGTCAATGGAATTGTTTTGTGTTCTCTTTGTATTATTATCATCATTAGTATTATGGTGCAACATTGTTAGGATTTTTAGTTTTTGTCTGGTTGGTGCATATAGTCAATTGTGGGTCGAAAACATCTGCTAGGACAACTAATGTGGATTTCAATTTATGCATAATATTTCTGACACCCTGAATTTGTTGGTAGATTTCTTCTGTWWTA
TARGET=424,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01153 Ps_contig_mira-and-tgicl-ass_franssen_10648
SEQUENCE=GAACTCGAGTAGCACGTGTGACAGTTAATACAAAGAGTAGCAAGCGCCGCAGTGCGTAATCACAAGGAAGACCGTTCGATCGAACACCACGTCATCGATCCGCGTGAAATCGTATCTGAACGGCTCAGATCATTTTCACAGAGTAACTGAAGAAACGRAGCGAAGAAGAGTCTGGGAACTTGGCTTCGTTGTTTCTCTACTCTCTCGTGCTCGATTTTCCACTCTTCAATGAAAACAGAATCTCAAATTTTTGTGGCGTCGAAGATGAATTTCACGGAGGATTCTGAGAAAGTGAAGAAAATTGAGGTTGTTGTTTTGAGTGAAGATGAAGGTGAAGACGATTATGAGTCAGTGGAAGAAGTAGACGCCGGAGAAGACGACGGTGATGAGGATGACGATGTTGATGATGAGGAGGAGGATGAAGAGGAGGAGGAGGAGGATGCTCCTGACGCCGGCAACGACGAGGATGATGATGACGAAGAGGAAGATGGAGATGACGAAGCAGGTGGTGGTGAGCGTGGTGGTGACTCTGATGATGATGACGACGACGACGAGAACGATGACGACGAGGAGGATAACGATGAAGAGGATGATTTGGGAACAGAGCACCTCGTTCGCCCTATAGGCAATGCTGAAGAGGAGGAAGCTTCTAGTGATTTTGACCCAGAAGAAAATGACGAGGATGAAGACGAAGATGATGATGAGAAGGATAAGGTTCCACCGAAAAGGAAGAGGTCAAATAAAGATGATGATTCCGATGACGATGATGGAGGTGAAGATGATGAAAGACCCTCGAAAAAATAGAGTTGGGCTATTTCTTTGTTTGAGGACTTAACAAACCAAACCATAGTTGAAGTAGATCAGCATCTGTCCTTTGAACGCTTTTCAGAGAAAGGATGAGTAGGTCAATGGAATTGTTTTGTGTTCTCTTTGTATTATTATCATCATTAGTATTATGGTGCAACATTGTTAGGATTTTTAGTTTTTGTCTGGTTGGTGCATATAGTCAATTGTGGGTCGAAAACATCTGCTAGGACAACTAATGTGGATTTCAATTTATGCATAATATTTCTGACACCCTGAATTTGTTGGTAGATTTCTTCTGTWWTA
TARGET=531,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01154 Ps_contig_mira-and-tgicl-ass_franssen_10648
SEQUENCE=GAACTCGAGTAGCACGTGTGACAGTTAATACAAAGAGTAGCAAGCGCCGCAGTGCGTAATCACAAGGAAGACCGTTCGATCGAACACCACGTCATCGATCCGCGTGAAATCGTATCTGAACGGCTCAGATCATTTTCACAGAGTAACTGAAGAAACGRAGCGAAGAAGAGTCTGGGAACTTGGCTTCGTTGTTTCTCTACTCTCTCGTGCTCGATTTTCCACTCTTCAATGAAAACAGAATCTCAAATTTTTGTGGCGTCGAAGATGAATTTCACGGAGGATTCTGAGAAAGTGAAGAAAATTGAGGTTGTTGTTTTGAGTGAAGATGAAGGTGAAGACGATTATGAGTCAGTGGAAGAAGTAGACGCCGGAGAAGACGACGGTGATGAGGATGACGATGTTGATGATGAGGAGGAGGATGAAGAGGAGGAGGAGGAGGATGCTCCTGACGCCGGCAACGACGAGGATGATGATGACGAAGAGGAAGATGGAGATGACGAAGCAGGTGGTGGTGAGCGTGGTGGTGACTCTGATGATGATGACGACGACGACGAGAACGATGACGACGAGGAGGATAACGATGAAGAGGATGATTTGGGAACAGAGCACCTCGTTCGCCCTATAGGCAATGCTGAAGAGGAGGAAGCTTCTAGTGATTTTGACCCAGAAGAAAATGACGAGGATGAAGACGAAGATGATGATGAGAAGGATAAGGTTCCACCGAAAAGGAAGAGGTCAAATAAAGATGATGATTCCGATGACGATGATGGAGGTGAAGATGATGAAAGACCCTCGAAAAAATAGAGTTGGGCTATTTCTTTGTTTGAGGACTTAACAAACCAAACCATAGTTGAAGTAGATCAGCATCTGTCCTTTGAACGCTTTTCAGAGAAAGGATGAGTAGGTCAATGGAATTGTTTTGTGTTCTCTTTGTATTATTATCATCATTAGTATTATGGTGCAACATTGTTAGGATTTTTAGTTTTTGTCTGGTTGGTGCATATAGTCAATTGTGGGTCGAAAACATCTGCTAGGACAACTAATGTGGATTTCAATTTATGCATAATATTTCTGACACCCTGAATTTGTTGGTAGATTTCTTCTGTWWTA
TARGET=543,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01155 Ps_contig_mira-and-tgicl-ass_franssen_10649
SEQUENCE=CCTCCTCTTCAGCATTGCCTATAGGGTTTCGTAACGAGGTGCTCTGTTCCCAAATCATCCTCTTCATCGTTATCCTCCTCGTCGTCATCGTTCTCGTCGTCGTCGTCATCATCATCAGAGTCACCACCACGCTCACCACCACCTGCTTCGTCATCTCCATCTTCCTCTTCGTCATCATCATCCTCGTCGTTGCCGGCGTCAGGAGCATCCTCCTCCTCCTCYTCWTCCTCCTCCTCATCATCAACATCGTCATCCTCATCACCGTCGTCTTCTCCGG
TARGET=94,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01156 Ps_contig_mira-and-tgicl-ass_franssen_10649
SEQUENCE=CCTCCTCTTCAGCATTGCCTATAGGGTTTCGTAACGAGGTGCTCTGTTCCCAAATCATCCTCTTCATCGTTATCCTCCTCGTCGTCATCGTTCTCGTCGTCGTCGTCATCATCATCAGAGTCACCACCACGCTCACCACCACCTGCTTCGTCATCTCCATCTTCCTCTTCGTCATCATCATCCTCGTCGTTGCCGGCGTCAGGAGCATCCTCCTCCTCCTCYTCWTCCTCCTCCTCATCATCAACATCGTCATCCTCATCACCGTCGTCTTCTCCGG
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01157 Ps_contig_mira-and-tgicl-ass_franssen_10649
SEQUENCE=CCTCCTCTTCAGCATTGCCTATAGGGTTTCGTAACGAGGTGCTCTGTTCCCAAATCATCCTCTTCATCGTTATCCTCCTCGTCGTCATCGTTCTCGTCGTCGTCGTCATCATCATCAGAGTCACCACCACGCTCACCACCACCTGCTTCGTCATCTCCATCTTCCTCTTCGTCATCATCATCCTCGTCGTTGCCGGCGTCAGGAGCATCCTCCTCCTCCTCYTCWTCCTCCTCCTCATCATCAACATCGTCATCCTCATCACCGTCGTCTTCTCCGG
TARGET=208,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01158 Ps_contig_mira-and-tgicl-ass_franssen_10667
SEQUENCE=CCTTTCATCACAATTTTTTTTATTCATTTATGATACTGTCATGTCAGATTCACCAGTAAAATGAAGCAAAAATATTGGAAAGCATGGAAGTTTTAATAAACATTCATTGTTCCATTTTTTTTTGAAACATTTGAGTACATCATTTGGCAGATTTAACTATAGCTGCAATTAAAAAAACAAATTCCAAACTTAAAAAAAAGTGGAATTGTTCACAATGTAATTAGGAAGATTAACTAAGGAAGAAAAACAATTAAACAGACTCTTAATTAATCGACAGAACTCCGGTGTTAGCTGAAATTTTCCCTCTCTAAATCTAATCCTCTCTACGACTACAGCACCGTGCAACAGTGGTAGGAGATTCAAATTCGGTTGAGAGATGAATGTGTCAAGAGGCTAGGGGAAGTACCTGTGATGTGCTTGAATGGTACTGTTGTAGATGAGATTGATGATTATGATTAACCTCTCTTTCGTAACTCTTGTCAACAACGATTGTAATGAGAGAATCATCAACTTCGTTTCCGTTAAAACCCTTGTTGTTGTTGTTGTTGTTGTTATCTTGCATTTTTCTTCGAAGCTTCTCTTCGGCTTCTTGTAAGAACTTGAATTTCGGAGGAGGTGACGACTTTAATCTATTGAACTCTGCATCGGTTGTTGATTCGAAAAGAGGGTTGAATCCGTGTTGATTATAGGGACTAACATTGACGTTAGGAGTGAAAGTAGGAGTGAAAAAATGTGGAGATGAAATAGGAGTCAAATATGGTGTTTCAACATCTAACAAATCGCCTAAGCTTCTTGAATCCTTTCCACTTTTGTTTTCACATCTAGATTTACTATCTTCTGATTCCAAATCTTCTTTTGATTCTTCGACTATTGTGAATAAAATTCTCGGATGACCTAAAACGCCGTCGTCGTGAAGATGATGTTGCATCATGTATTCAGCTTCGACGGAATCTTCGCCGTAGGGCTTGAATAGAAACTCTTTGCTAGTTTGTACTTGAGGGTCATGGACAAAATTGTCATTCGTTCTCATTGAAGAACAAAGCTCCGGTGGAGTAAAACCTGTGTGTCTCATAGAACTTGATGATGATGGCCTTTTCCAACAAAACATATAGAAAAGCTCTTTCAAAGGGTTTCCATTTCCATAATCATTTTCAATCTCTCTATGGATTATCTTTTTCTTCCACCATAACAAGTAGTAAACTTCAGCTACTAAAGCCAATAAAAGACAACCAAAAACAATACTAAGACCAAGTCCTACACTACTCAAAGATTTCATCTTTGTTCTCAAATCAAACTTAAAAATCAAAATTTGAATCTAACGACGGTACATACTTCTCAGCCGAACTCCGGATTACCAGGTTGGTCAGTCGCCCTTTCGGTAAAAATCAAAGAGTTAAAACACAACTTTCTTGACCTCCAATGATGTTCATTGGCCAAAATGATTAACATCTAATCTAGTCATGTCAAATAGAGCAATGGAATACAAGAAAATTGGAAAATGAAAAAAATGCTTACTTGGGTAATTGACTTAATCCAAACCCTTATGCAAAGATTTTGAACTCAAACATGCAAATCTGGATATTCCTTTGACTAAAATTCAATTTCAATGTAAACCAATACCTCTTAGACATAATAACAAACACCTTGTGAGCAAATTGTAAAAGCTTCTCAAAAACACCAGGGACCATCCAAAAAAAAGCTCCTTTTTTCTCTCTTGAAAGCAACCCTTGCAAATCATAAGCTTGAAACTTTTTAGCTTTAGCCTTTAACCCCACAAGAGATTAGGCACCTCCTTATGGATTTGTCTCTTCATTGCCTTCAAAGATAATAGCAACATTTTCTAAAACACCATTGAAAAGAGAAAAATTCTCTCAATGAAAACCACTTTGTGGTCGTGCTAAAAAACCATGAGCAAGAATATAAACTAA
TARGET=531,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1680
PRIMER_LEFT_PENALTY=0.018835
PRIMER_RIGHT_PENALTY=0.149184
PRIMER_LEFT_SEQUENCE=GCTAGGGGAAGTACCTGTGATG
PRIMER_RIGHT_SEQUENCE=TTACAAGAAGCCGAAGAGAAGC
PRIMER_LEFT=392,22
PRIMER_RIGHT=594,22
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=60.149
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=0.1688
PRIMER_LEFT_1_PENALTY=0.087340
PRIMER_RIGHT_1_PENALTY=0.081412
PRIMER_LEFT_1_SEQUENCE=CAACTTCGTTTCCGTTAAAACC
PRIMER_RIGHT_1_SEQUENCE=CAAGAAGCTTAGGCGATTTGTT
PRIMER_LEFT_1=507,22
PRIMER_RIGHT_1=794,22
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=59.919
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.2502
PRIMER_LEFT_2_PENALTY=0.018835
PRIMER_RIGHT_2_PENALTY=0.231323
PRIMER_LEFT_2_SEQUENCE=GCTAGGGGAAGTACCTGTGATG
PRIMER_RIGHT_2_SEQUENCE=CAACCGATGCAGAGTTCAATAG
PRIMER_LEFT_2=392,22
PRIMER_RIGHT_2=650,22
PRIMER_LEFT_2_TM=60.019
PRIMER_RIGHT_2_TM=59.769
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.2681
PRIMER_LEFT_3_PENALTY=0.018835
PRIMER_RIGHT_3_PENALTY=0.249232
PRIMER_LEFT_3_SEQUENCE=GCTAGGGGAAGTACCTGTGATG
PRIMER_RIGHT_3_SEQUENCE=CGAAGAGAAGCTTCGAAGAAAA
PRIMER_LEFT_3=392,22
PRIMER_RIGHT_3=583,22
PRIMER_LEFT_3_TM=60.019
PRIMER_RIGHT_3_TM=60.249
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=0.2908
PRIMER_LEFT_4_PENALTY=0.141638
PRIMER_RIGHT_4_PENALTY=0.149184
PRIMER_LEFT_4_SEQUENCE=AAGAGGCTAGGGGAAGTACCTG
PRIMER_RIGHT_4_SEQUENCE=TTACAAGAAGCCGAAGAGAAGC
PRIMER_LEFT_4=387,22
PRIMER_RIGHT_4=594,22
PRIMER_LEFT_4_TM=60.142
PRIMER_RIGHT_4_TM=60.149
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_01159 Ps_contig_mira-and-tgicl-ass_franssen_10676
SEQUENCE=ACTTAAACGCTCACCTACTCTTGCCGCCTTCCAGAAGAAACCTCGAAATCCAGTTCTCTGTTCAAGGATAGCTGGARATTGCATCTTGAAGTAAGTACAGGCACATATTCCCAAAAGTATGACAGTAAGAAATGAATGGAAATTGAAAAGCGCAGACATCTTCAACTATTCTTATAATTCACCACTCTTCACCTTCTTCTTCTTCTTCTTCCCTCGTTATAACTCTGAATTCGAWGGTGTGATCGTCGATCGGTGAGAACGAAAAATT
TARGET=193,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01160 Ps_contig_mira-and-tgicl-ass_franssen_10677
SEQUENCE=TCGTATCTAATCCACTCCAAATATCTTAAGGGTATAGTTAGAAGAAGAAAGATGTCAACCGTATCTTGAATTTCTTACACACTCAATCAAATGCTTAAACTCAACCTAGTTTTTAAGGAAAGAGCAAGCACATTCTACAGATACCATTGTACACTGTGTCTGAAAAAGTTACATAAAACTATCAGAAGAAAAATCAGTTAATCGGATTCAAGCTACTACAATGAAAAAGAGCTAAACAGTGGAGTTTCTGGGGATTCAATTAAATAATTTTAGGTACAAACTATTATATTGTAGATCTGCAGACCATGTGTCACACAAAACTGAAGTAGAGCAATAGAATGGATTCCCTATTGAGTAGACGGAGACAGAGCTTAGAAGAAGATTATTGAAACACCCATCATGAAACAACCCATAGCTACCCACGGACTTAAACGCTCACCTACTCTTGCCGCCTTCCAGAAGAAACCTCGAAATCCAGTTCTCTGTTCAAGGATAGCTGGAAATTGCATCTTGAAGTAAGTACAGGCACATATTCCCAAAAGTATGACAGTAAGAAATGAATGGAAATTGAAAAGCGCAGACATCTTCAACTATTCTTATAATTCACCACTCTTCACCTTCTTCTTCTTCTTCTTCTTCCCTCGTTATAACTCTGAATTCGAAGGTGTGATCGTCGATCGGTGAGCGAAAAATTGATTCAGCTTGCGGTTCCMAGTAATGTTGCTTTACCCCG
TARGET=618,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01161 Ps_contig_mira-and-tgicl-ass_franssen_10680
SEQUENCE=ACAAGACAGGAATATGTTGCTCATTTAAGAAGAAAAAGCAGCGGCTTCTCAAGAGGTGCTTCCATGTACAGAGGAGTAACAAGACACCATCAACATGGAAGATGGCAGGCTAGAATTGGTAGGGTGGCTGGTAACAAAGACCTATATCTTGGAACCTTCAGTACTCAAGAGGAAGCGGCTGAGGCCTACGACATAGCTGCGATAAAATTCAGAGGAGCTAATGCAGTAACCAACTTTGATATAATAAAATATGATGTTAGAAAAAATCATGGCAAGTAGTAACCTTCTAAGCATTGAACTAGCAAGAAGGAACAAAGAGGTAGTAAATATTACCACAACTCAATATATTGAACCTCTCAACAACACTACTCAAGAGGCCATGCAGAAGAATATGATTCTCTATCAATCCCCTCAACAACAACAACAACTTCAACAGAATCAACCAAGAATCGAGAACGAGAGAACTCATCAATCATTTTCCTCGGTGTCTTTGGATAACATGTTTCATCAAGAAGTAGCCGAGGAAGCAAGTAAGATGAGAGCGCATTTGTCAAATGCTTCTTCATTGGCCACAAGTTTGAGTAGCTCAAGAGAAGGTAGCCCTGATAGAACAAGCTTGCAGAATCTCTCTGGAATTCCTTCAACATCATCGAAACTATTGGTTGCGACCG
TARGET=413,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0525
PRIMER_LEFT_PENALTY=0.042194
PRIMER_RIGHT_PENALTY=0.010294
PRIMER_LEFT_SEQUENCE=AGGCCATGCAGAAGAATATGAT
PRIMER_RIGHT_SEQUENCE=ATTCCAGAGAGATTCTGCAAGC
PRIMER_LEFT=374,22
PRIMER_RIGHT=635,22
PRIMER_LEFT_TM=59.958
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=0.0837
PRIMER_LEFT_1_PENALTY=0.042194
PRIMER_RIGHT_1_PENALTY=0.041553
PRIMER_LEFT_1_SEQUENCE=AGGCCATGCAGAAGAATATGAT
PRIMER_RIGHT_1_SEQUENCE=TCTTACTTGCTTCCTCGGCTAC
PRIMER_LEFT_1=374,22
PRIMER_RIGHT_1=535,22
PRIMER_LEFT_1_TM=59.958
PRIMER_RIGHT_1_TM=60.042
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.0837
PRIMER_LEFT_2_PENALTY=0.042194
PRIMER_RIGHT_2_PENALTY=0.041553
PRIMER_LEFT_2_SEQUENCE=AGGCCATGCAGAAGAATATGAT
PRIMER_RIGHT_2_SEQUENCE=TACTTGCTTCCTCGGCTACTTC
PRIMER_LEFT_2=374,22
PRIMER_RIGHT_2=532,22
PRIMER_LEFT_2_TM=59.958
PRIMER_RIGHT_2_TM=60.042
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.1595
PRIMER_LEFT_3_PENALTY=0.042194
PRIMER_RIGHT_3_PENALTY=0.117281
PRIMER_LEFT_3_SEQUENCE=AGGCCATGCAGAAGAATATGAT
PRIMER_RIGHT_3_SEQUENCE=TCGGCTACTTCTTGATGAAACA
PRIMER_LEFT_3=374,22
PRIMER_RIGHT_3=521,22
PRIMER_LEFT_3_TM=59.958
PRIMER_RIGHT_3_TM=59.883
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=0.2366
PRIMER_LEFT_4_PENALTY=0.042194
PRIMER_RIGHT_4_PENALTY=0.194358
PRIMER_LEFT_4_SEQUENCE=AGGCCATGCAGAAGAATATGAT
PRIMER_RIGHT_4_SEQUENCE=GACACCGAGGAAAATGATTGAT
PRIMER_LEFT_4=374,22
PRIMER_RIGHT_4=488,22
PRIMER_LEFT_4_TM=59.958
PRIMER_RIGHT_4_TM=60.194
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.5000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_01162 Ps_contig_mira-and-tgicl-ass_franssen_10693
SEQUENCE=CTTGGATGATGAACTGCCGAGATTACATCGTCAGGACTCTCCACACTACAAGCCACCATTGATTTCTTCAAAGCAGCCAACATTAGCGCCGCCGGCGAAACTTCCTCGCCGCCGCCGCCGTCACGTCCTCCTCTTCTTCCCTTCACACCACCACCACCGCATCCACCACTCACCATCACTGGTCCTCTCATTCTCAATTATCCTCTTGTTATTCTCCACACAAACACTATCTCTAGAGASMCMGYATTTTGAATTCGG
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01163 Ps_contig_mira-and-tgicl-ass_franssen_10693
SEQUENCE=CTTGGATGATGAACTGCCGAGATTACATCGTCAGGACTCTCCACACTACAAGCCACCATTGATTTCTTCAAAGCAGCCAACATTAGCGCCGCCGGCGAAACTTCCTCGCCGCCGCCGCCGTCACGTCCTCCTCTTCTTCCCTTCACACCACCACCACCGCATCCACCACTCACCATCACTGGTCCTCTCATTCTCAATTATCCTCTTGTTATTCTCCACACAAACACTATCTCTAGAGASMCMGYATTTTGAATTCGG
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01164 Ps_contig_mira-and-tgicl-ass_franssen_10700
SEQUENCE=TCGTGGATTTTTTCTCGGCTAGTAGAGTCAGTGTGGTGGCAGGCTTTGACTCCTCATATGCAATCTTCGGTTGTGGACTCGTGTTCAAATAAATCCATTGGGAGGCTGCTAGGGCCTGCTTTAGGTGATCACAATCAAGGAAACTTTTCCATCAATCTCTGGAGAAACGCTTTCCAAGACGCGTTTCAAAGACTCTGTCCTGTTCGAGCAGGAGGGCACGAGTGTGGTTGTTTGCCTGTGATGGCAAGAATGGTCATGGAACAGTGTGTAGATAGACTAGATGTTGCGATGTTCAATGCAATTCTTCGAGAATCAGCCCATGAGATTCCAACTGATCCTATATCAGACCCGATTGTCGATTCCAAGGTTTTGCCAATTCCAGCAGGAAACTTAAGCTTTGGATCTGGTGCACAACTGAAAAATTCCGTTGGCAATTGGTCGAGATTGCTCACTGATATGTTCGGCATTGATGCTGAAGATTGTCTAGAAGAATATCAGGAGAACGGTGAGAACGACGAAAGGCAAAGCGGAGACGGTGAACGTAAATCATTTGCTCTCCTTAACGACTTGAGTGACCTTCTAATGCTCCCAAAAGACATGCTTATAGAAAAACAAGTTAGACATGAGGTATGTTCATCCATCAGTCTTTCTTTGATTATACGCGTTCTATGTAACTTCACACCGGACGAGTTCTGTCCCGACCCGGTTCCAGGAACTGTCTTGGAGGCCTTGAATGGAGAGACTTTTGTGGAGCGAAGACTGTCGGCGGAATCCATTAGAAGCTTCCCTTATGCTGCGGCTCCAGTTGCGTATACACCTCCCTCTTCAGCCAATGTGGCGGAGAAAGTTGCAGAGGCGGGAGGAAAGTCTCACCTGACGAGGAATGTATCGGTTGTCCAGAGGAGAGGATATACAAGTGACGAAGAACTTGAGGAACTTGAATCGCCGATCGCGTCTATCATTGATAAGGTTCCTTCTTCTCCAACAGTTACTACTAATGGAAATGGTAATCACAAGGAGCAAGGGAGTAACATTACAACAAATGCTAGATATCAACTACTTCGTGAGGTTTGGTCCATGTGATGTGATGTGAACCTTGTAGAAACGAAATCAACATGTATACTACTTAACAAAGTAAATAAGTAAGGAAAAAAATGTTAGAAAACCTTCCCATACAAATCCACCACCGTTACACGGTTGTTCTTT
TARGET=1078,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4761
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.345718
PRIMER_LEFT_SEQUENCE=GTAATCACAAGGAGCAAGGGAG
PRIMER_RIGHT_SEQUENCE=GTGTAACGGTGGTGGATTTGTA
PRIMER_LEFT=1006,22
PRIMER_RIGHT=1194,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=59.654
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.4761
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.345718
PRIMER_LEFT_1_SEQUENCE=ATCACAAGGAGCAAGGGAGTAA
PRIMER_RIGHT_1_SEQUENCE=GTGTAACGGTGGTGGATTTGTA
PRIMER_LEFT_1=1009,22
PRIMER_RIGHT_1=1194,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.654
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=0.4761
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.345718
PRIMER_LEFT_2_SEQUENCE=TAATCACAAGGAGCAAGGGAGT
PRIMER_RIGHT_2_SEQUENCE=GTGTAACGGTGGTGGATTTGTA
PRIMER_LEFT_2=1007,22
PRIMER_RIGHT_2=1194,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=59.654
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.4761
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.345718
PRIMER_LEFT_3_SEQUENCE=AATCACAAGGAGCAAGGGAGTA
PRIMER_RIGHT_3_SEQUENCE=GTGTAACGGTGGTGGATTTGTA
PRIMER_LEFT_3=1008,22
PRIMER_RIGHT_3=1194,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=59.654
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.5044
PRIMER_LEFT_4_PENALTY=0.158697
PRIMER_RIGHT_4_PENALTY=0.345718
PRIMER_LEFT_4_SEQUENCE=GGAAATGGTAATCACAAGGAGC
PRIMER_RIGHT_4_SEQUENCE=GTGTAACGGTGGTGGATTTGTA
PRIMER_LEFT_4=999,22
PRIMER_RIGHT_4=1194,22
PRIMER_LEFT_4_TM=59.841
PRIMER_RIGHT_4_TM=59.654
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=196
=
PRIMER_SEQUENCE_ID=SSR_01165 Ps_contig_mira-and-tgicl-ass_franssen_10725
SEQUENCE=TTTGTAATCAAAGGTGACGATATTGAACAAGAAAACCAAGAGATTCTTCAGGTATGCAATTCTTGGATCCTCGATCTTGCCTTCTTCTTCTTCTTGGTGACTTTAGTCTGTAGGAAGTGATGRGGGATTTCAATTAAACCCTTGCGTGAGAGTAAGGAGAGGGATCTGTTGAATAAGGTTCAAGCTTAACCGATTGAAACTTCTACGTGRAGCTTCACTGGCCTAGTAATGGAAGCTTTAGGTAGAGGGTGWGTTCAGAGAGGGAGAGAATT
TARGET=81,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01166 Ps_contig_mira-and-tgicl-ass_franssen_10738
SEQUENCE=GATSACCCTTTTCACATTCTTCTTCTTCTCCTCACACAGATCTTCATCTCACCTTTCACTTTCTCTCTCTCAATTCACTCTCTGAGGTGAAACTGTTTCTGGGTTTTGGTCTTGGATCCTTCAAAAGACTCAAACTTTCATTCTGCTGTTCAAAATGTGGATCTTGTAAAAGACTTATTGATTTCATCAAGAAGAAATTA
TARGET=17,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01167 Ps_contig_mira-and-tgicl-ass_franssen_10738
SEQUENCE=GATSACCCTTTTCACATTCTTCTTCTTCTCCTCACACAGATCTTCATCTCACCTTTCACTTTCTCTCTCTCAATTCACTCTCTGAGGTGAAACTGTTTCTGGGTTTTGGTCTTGGATCCTTCAAAAGACTCAAACTTTCATTCTGCTGTTCAAAATGTGGATCTTGTAAAAGACTTATTGATTTCATCAAGAAGAAATTA
TARGET=62,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01168 Ps_contig_mira-and-tgicl-ass_franssen_10740
SEQUENCE=CGGGGCAACCCGGCTGCACAAGCGAGTTTGTTGTTGGGGATGTTTCCTTGGAGAGGGTATACACTTGTGGAGGAGARTGGTTCTTTGAAGCTTGGTTGGAAAGATCTTTCTCTTTTGATTGCGTCTGCTTGGCAACCGTCTGATTTGATTACTTACACTTGATGATGATGATGATGAGTGGCGTATTGTTACTAGTTTAGGTTTTGGTTCGGTTCCGGGGGTTTTAACGGGGGGGGTTAAAGGGGGGGGGTTAAAAATTTGGTTTAAGGTTAAACTTTTAACGGTTTTTCCCTCCTTACGTACGTTCCGGT
TARGET=160,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01169 Ps_contig_mira-and-tgicl-ass_franssen_10741
SEQUENCE=GCAACTTTTTTCTTTGACAACCATAATGAACATTGCAMAAAAAAAGGAACAGAAAGCTATGAACACACACGAGCTCTTTGCGCTTCTCTTTCTTCCAACCCAACAATTAAAGAATTTTCGTCTCTTTCAGATAAACATCTTTTATATGGTACAAACCCTCTTCCAGATTTCTTGAGGAACACTCCTTCATGATGATGATGTTTTCGGCTTTGAGTATCGTCGGTATCTGATGAACTGTTCTTGCTTTGATTTTCCGTATCACAAACTGATCCGGTATTAGAACCTGTGCAAGAACTTTCCTCCTCTCGAGTTCTCACCTTCAAACAAGGCCTGAGAGGCACAGGACTTAAAATCTGATGATTGCATGGCTTGAGGCTCAAAGCGGGAAGACCTTGGTACAAACTCCAACATGGTAAAGATGATGCGCATTCACCGCCGCCGCACGGATTCTCCTTCGGTTGTTCCATACTGTCACAATTACCACTACACATGCCTAGTGATAGTCGTGTAACAAGTTTCTCTGATTCCTCTTCTAAGCCAACTTTCTCATTCTCAACAATTTCATCAGATTTGATGTCTTCTACTGTTTTCTGAGTATCAACCATAGAGACTTGTTCTCGACCAAACTAAGTTTTAATACTAGTGATGTGTGGCATATCCGCAGCATCTTCCTTGAAACATTGAGTTTCTTCCGAAGAAATAATCTCGGGCTTCGTGCATATAAGTGGTTTTGAGTCGGTGGATAAAGGAACTGAAGCTAGAGTTCCTTTCTCTTCCT
TARGET=189,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01170 Ps_contig_mira-and-tgicl-ass_franssen_10743
SEQUENCE=TCTGGTACAAGAAGATGGAAAACTGTGTAACTCCTGCCAAACCTAGTGGTTCGTGGAAGCCATTCCCTGAGAGACTCAATGCTGTTCCTTCGAGAATCACCAGTGGATCCATTCCCGGTGTATCCGCAGAGTTATTTGAGGAGGACAACCGATTATGGAAGAAACATGTGAATGCTTACAGGCGTGTCAACAAAATCATCGACTCGGGGAGGTATCGCAATGTGATGGACATGAATGCTGGTTTTGGAGGTTTTGCTGCGGCCATAGACTCTCCCAAATTATGGGTTATGAATGTCGCGCCTACGATTTCTGAGAAAGCTAGCCTTGGTGTTGTCTTCGAGCGAGGATTGATTGGCATTTATCATGATTGGTGTGAAGCCTTCTCCACATATCCAAGGACCTATGACCTTATCCATGCAAATGGTGTTTTCACCTTGTACAAAAATGCGTGTAATGCAGAAGATATTCTATTGGAGATGGACCGCATCTTACGGCCCGAGGGAGCGGTAATATTCCGAGATCAGGTTGGAGTGCTGAAGCAAGTTAAAAGAATTGCAAAAGGAATGAGATGGAATACCAAAATGGTGGATCATGAAGATGGTCCACTGATTTCAGAGAAGGTGTTGTATGCCGTCAAAAGATATTGGGTTGCCGGTGACAACACCACAAGCTCAGGGTAGTTCCAAGGTTTCAGATGACATGATGATTCTGATTTTGATTTTTTTGGGGCATGAAGTTTTCTAGATTCGTAACATCAGTGAGCATATATTTCTTGTTTTGTTGAATGTAAAGTTGATAGATGTCTTATTTTTTGGAGATTATAAATTGTAGTAGTCGCTGATCATATTTATTTATTTGTTTKGKTYACGAAACGTT
TARGET=845,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01171 Ps_contig_mira-and-tgicl-ass_franssen_10765
SEQUENCE=AGAAAGGCTTTCTATAAACATCAAATGCTTCGCCAGCTGTTAACTGTGGTTCCTGTCTCAGCCCACCAGCAGTAAGACTACCTCCAGTCAAAAGCATGGAAAGAAGAGATGTTACCACATCGCCGCCCCCACTACCACCACCACCGCTGCCAGCATTTTTAATAGCAGTGCGCATTGCAGTAAGAATGGTGCCATATGTGGCACCTGGACCAGAGCGTTCTATTGCTTGGATGAAGCAAAATGTCATGGCTCCAGTTGATGTAATTTTAGATAGTGCAGATGTATCTGCAGAAGTTTGATGATCATCGCATCCACTAAAACTTATAACTTCTCCACCACTTGATCCCTTCCATACACCTGACCTAGGGCGATGGTCCTCCCATACATATTGTCCACGGCTGTTCATTCTGCAAAGAAATGGCAAATCCATAACTGTGCCACTATGGCAGGCATCAATTAGTGCATGCAGCCTAACCCCGTGAGTAAGAGGCCTTACGAGCGTTGCATTGATCTCAATCATCTACTATCATACCCTGTG
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2254
PRIMER_LEFT_PENALTY=0.162046
PRIMER_RIGHT_PENALTY=0.063381
PRIMER_LEFT_SEQUENCE=AGCCCACCAGCAGTAAGACTAC
PRIMER_RIGHT_SEQUENCE=CGCACTGCTATTAAAAATGCTG
PRIMER_LEFT=59,22
PRIMER_RIGHT=171,22
PRIMER_LEFT_TM=59.838
PRIMER_RIGHT_TM=59.937
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.1000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=113
PRIMER_PAIR_PENALTY_1=0.2280
PRIMER_LEFT_1_PENALTY=0.164595
PRIMER_RIGHT_1_PENALTY=0.063381
PRIMER_LEFT_1_SEQUENCE=CAGCTGTTAACTGTGGTTCCTG
PRIMER_RIGHT_1_SEQUENCE=CGCACTGCTATTAAAAATGCTG
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=171,22
PRIMER_LEFT_1_TM=59.835
PRIMER_RIGHT_1_TM=59.937
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=139
PRIMER_PAIR_PENALTY_2=0.3647
PRIMER_LEFT_2_PENALTY=0.301367
PRIMER_RIGHT_2_PENALTY=0.063381
PRIMER_LEFT_2_SEQUENCE=CCCACCAGCAGTAAGACTACCT
PRIMER_RIGHT_2_SEQUENCE=CGCACTGCTATTAAAAATGCTG
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=171,22
PRIMER_LEFT_2_TM=59.699
PRIMER_RIGHT_2_TM=59.937
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=0.6430
PRIMER_LEFT_3_PENALTY=0.579590
PRIMER_RIGHT_3_PENALTY=0.063381
PRIMER_LEFT_3_SEQUENCE=CCAGCTGTTAACTGTGGTTCCT
PRIMER_RIGHT_3_SEQUENCE=CGCACTGCTATTAAAAATGCTG
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=171,22
PRIMER_LEFT_3_TM=60.580
PRIMER_RIGHT_3_TM=59.937
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=140
PRIMER_PAIR_PENALTY_4=0.6635
PRIMER_LEFT_4_PENALTY=0.164595
PRIMER_RIGHT_4_PENALTY=0.498888
PRIMER_LEFT_4_SEQUENCE=CAGCTGTTAACTGTGGTTCCTG
PRIMER_RIGHT_4_SEQUENCE=CATATGGCACCATTCTTACTGC
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=197,22
PRIMER_LEFT_4_TM=59.835
PRIMER_RIGHT_4_TM=59.501
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_01172 Ps_contig_mira-and-tgicl-ass_franssen_10767
SEQUENCE=CGAAGAAAAACAAACAACTTCAAGTCGAAATCAAAATGCTCTCTCTCTCTCAACAAATATATGAAAACGACGATACAGAGTGATACAAAAATGCAAACATGGGAAAGGAACAAGAAACAAACCGGCAGAAGGCGAAGTTTGGTGGTGATTTCGTACGCGGTTTCCAGAATGGAGGGTGTTGGATTAGGAAGCTTC
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01173 Ps_contig_mira-and-tgicl-ass_franssen_10779
SEQUENCE=CCGGTAGTCAATACATATTAAAAAAAGGTTTAAAGGKCGAAGTAATAGCTCATCGGAATAGCAGTTGCGGTTTCCCTCCATATTCGGCCTGCAATGATGTTTGAATTTTGTAAAATAGAGGCTGTCTGCCATAGTTATTATAGCATGCTGTCGTTTCATGGAGAACCACTGTGAACCCTTTCGAAAATCTTTCTTTTCTACTTCAGGCAACATACGTTCCATATACCTGCCATTTCCATGAGGACCGAGATCCACATAGCTATCAATAAAGCTGACACTTGTTAATAACAAGTAGTTGTACACAAAATCAAAGCGGCGCACCGGAATACAACTATCAGACAACAAGACGAAATGTTGGTTATCAGGGTCTAAAAGCGCATTTGCCAAAAGTCTCCTTTCAGCTTCAACCATTGAAACTTTTCCCCAAGTTACCGGTTCACTGTGGATGTCTCGACCAACAAAATAGGAGCTGACATGTACCGGTTTTTCCTTCGATGCATGTACATAGACAGTGAATTTGCCTTCATGGCCCTGGAAGAACATGTGCCATAGTTTCTCGAAAGRTAATGAACCCGGAGTCAAAAAACAAAAACGCAACTTTAGGGACCTTGGTTTGAACAGGATAATACTTAAGAATTTCGTTAATAACAACCCGAGATTCAACTTCTGCATCCGAGAATTCCCGTGTCTGTACAGGTGGTGGCCGACTATACGTACTACCACTACCACCAACAAAACTACAACCTTGAGGGTTAAAGAAAGAACACGTCGAGGAGAGGCTCCACAGCGGATAAATATAAGCAGCAATCAGAAAAAAAACACWAYGAAGACTAAAACAACAATCCAATTAGGCCTCTTTAAGTGTGATCGGGGTCTTGATCCAACCATGATCAAATGACTTCTTATGAACTGCGGCCATGCAAGTTCTTTCTTCATTTAAGTTCAAGACATTCCTTCACGTTCCCCGAATGAATCCGTAGTCGAACGACAACATTCATTTACATTCAACCCTGTGATTGAATGCAGGGATCTGAGGAAGAACCCTGCAGAAAAAATGGAAACGGAAGAATGGAAGACAATGTCAACGTTGGTGTTTTTAGGTTGTGTCGTGGTTGGATTGGATTGGATCGGTGGTTTAGATTTGTTATGACAAATGGAGAGCGTTGATTCCGGGGTTTTGTGTTGYGTTGTTGAATATTTTAAGTTTAACTTGTATTTGTSGATGTGTTTGGGTTTTTGATTATTTTTGTATGTCTCTCTCTTCCCCGTCGCTGTTTTGTTG
TARGET=1113,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01174 Ps_contig_mira-and-tgicl-ass_franssen_10791
SEQUENCE=GTCGGGGAGGAACCAAACCATGAACTCTCTCTCCCTCCACAACGCTAATGCAACCACAACACACACACTCCACTTCCACTCTCACCACACCTTCATCCACTCCAAACCCACCCCTTCCTTCCTCTTCCTCAAATCAAAACCCCCAACCCATACACAATGCTGCATCAACAACAACAACAGCAACAACATTATCGACATCGACATGGTAAAAACCAAACAAGGAACCTACGTACCAAAACAGAACAAAGTCGTTGTTCTCTGGGACCTCGACAACAAGCCCCCACGCGGTCCACCTTA
TARGET=59,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1348
PRIMER_LEFT_PENALTY=0.110131
PRIMER_RIGHT_PENALTY=0.024665
PRIMER_LEFT_SEQUENCE=CAAACCATGAACTCTCTCTCCC
PRIMER_RIGHT_SEQUENCE=CGATGTCGATAATGTTGTTGCT
PRIMER_LEFT=13,22
PRIMER_RIGHT=199,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=60.025
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.1348
PRIMER_LEFT_1_PENALTY=0.110131
PRIMER_RIGHT_1_PENALTY=0.024665
PRIMER_LEFT_1_SEQUENCE=CCAAACCATGAACTCTCTCTCC
PRIMER_RIGHT_1_SEQUENCE=CGATGTCGATAATGTTGTTGCT
PRIMER_LEFT_1=12,22
PRIMER_RIGHT_1=199,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.025
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=188
PRIMER_PAIR_PENALTY_2=0.1787
PRIMER_LEFT_2_PENALTY=0.110131
PRIMER_RIGHT_2_PENALTY=0.068602
PRIMER_LEFT_2_SEQUENCE=CAAACCATGAACTCTCTCTCCC
PRIMER_RIGHT_2_SEQUENCE=ATTTGAGGAAGAGGAAGGAAGG
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=133,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.069
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=121
PRIMER_PAIR_PENALTY_3=0.1787
PRIMER_LEFT_3_PENALTY=0.110131
PRIMER_RIGHT_3_PENALTY=0.068602
PRIMER_LEFT_3_SEQUENCE=CCAAACCATGAACTCTCTCTCC
PRIMER_RIGHT_3_SEQUENCE=ATTTGAGGAAGAGGAAGGAAGG
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=133,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=60.069
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=0.2769
PRIMER_LEFT_4_PENALTY=0.252279
PRIMER_RIGHT_4_PENALTY=0.024665
PRIMER_LEFT_4_SEQUENCE=CATGAACTCTCTCTCCCTCCAC
PRIMER_RIGHT_4_SEQUENCE=CGATGTCGATAATGTTGTTGCT
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=60.252
PRIMER_RIGHT_4_TM=60.025
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_01175 Ps_contig_mira-and-tgicl-ass_franssen_10791
SEQUENCE=GTCGGGGAGGAACCAAACCATGAACTCTCTCTCCCTCCACAACGCTAATGCAACCACAACACACACACTCCACTTCCACTCTCACCACACCTTCATCCACTCCAAACCCACCCCTTCCTTCCTCTTCCTCAAATCAAAACCCCCAACCCATACACAATGCTGCATCAACAACAACAACAGCAACAACATTATCGACATCGACATGGTAAAAACCAAACAAGGAACCTACGTACCAAAACAGAACAAAGTCGTTGTTCTCTGGGACCTCGACAACAAGCCCCCACGCGGTCCACCTTA
TARGET=166,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0530
PRIMER_LEFT_PENALTY=0.028296
PRIMER_RIGHT_PENALTY=0.024665
PRIMER_LEFT_SEQUENCE=AACACACACACTCCACTTCCAC
PRIMER_RIGHT_SEQUENCE=CGATGTCGATAATGTTGTTGCT
PRIMER_LEFT=57,22
PRIMER_RIGHT=199,22
PRIMER_LEFT_TM=59.972
PRIMER_RIGHT_TM=60.025
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=143
PRIMER_PAIR_PENALTY_1=0.0983
PRIMER_LEFT_1_PENALTY=0.073614
PRIMER_RIGHT_1_PENALTY=0.024665
PRIMER_LEFT_1_SEQUENCE=ACTTCCACTCTCACCACACCTT
PRIMER_RIGHT_1_SEQUENCE=CGATGTCGATAATGTTGTTGCT
PRIMER_LEFT_1=71,22
PRIMER_RIGHT_1=199,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.025
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.1348
PRIMER_LEFT_2_PENALTY=0.110131
PRIMER_RIGHT_2_PENALTY=0.024665
PRIMER_LEFT_2_SEQUENCE=CAAACCATGAACTCTCTCTCCC
PRIMER_RIGHT_2_SEQUENCE=CGATGTCGATAATGTTGTTGCT
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=199,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.025
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=0.1348
PRIMER_LEFT_3_PENALTY=0.110131
PRIMER_RIGHT_3_PENALTY=0.024665
PRIMER_LEFT_3_SEQUENCE=CCAAACCATGAACTCTCTCTCC
PRIMER_RIGHT_3_SEQUENCE=CGATGTCGATAATGTTGTTGCT
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=199,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=60.025
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=0.2259
PRIMER_LEFT_4_PENALTY=0.201241
PRIMER_RIGHT_4_PENALTY=0.024665
PRIMER_LEFT_4_SEQUENCE=CACACTCCACTTCCACTCTCAC
PRIMER_RIGHT_4_SEQUENCE=CGATGTCGATAATGTTGTTGCT
PRIMER_LEFT_4=63,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=59.799
PRIMER_RIGHT_4_TM=60.025
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_01176 Ps_contig_mira-and-tgicl-ass_franssen_10812
SEQUENCE=GSACAGAGACAAACATTGTATTACCCGAAAAACCGTATACATTAGAAAAGAAAAGAAAAAAATAAGACAGGAAGAAAAAAGYAATTATCTTTAACATGAGGAAGRGAAACTACATTTCAAACTCAAATATTATCTGTAAACAAATTACTTTTCAAAGAGATAAATAATCACAACTATTTTTTACAAACAGTTGTACATTATGGTGTACAAAACTTGTTGCATGGAAATTATGGCGAAACTCGATCGCCATCGAT
TARGET=44,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01177 Ps_contig_mira-and-tgicl-ass_franssen_10830
SEQUENCE=AGATCCGTCTCTGTTTGGTATGAATAATTATTAACTATAAACATCCCAAATTCACAAGGAACCAGCAAGATAATCCACAAATCAAATTACTGAAAGAAAGAAAAATAATAGAATCAATTTTTTTTAATAATCCACCGCAACATAGAGAGA
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2659
PRIMER_LEFT_PENALTY=0.513831
PRIMER_RIGHT_PENALTY=0.752117
PRIMER_LEFT_SEQUENCE=AAATTCACAAGGAACCAGCAAG
PRIMER_RIGHT_SEQUENCE=CTCTCTATGTTGCGGTGGATTA
PRIMER_LEFT=47,22
PRIMER_RIGHT=148,22
PRIMER_LEFT_TM=60.514
PRIMER_RIGHT_TM=59.248
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=1.6966
PRIMER_LEFT_1_PENALTY=0.944463
PRIMER_RIGHT_1_PENALTY=0.752117
PRIMER_LEFT_1_SEQUENCE=GATCCGTCTCTGTTTGGTATGA
PRIMER_RIGHT_1_SEQUENCE=CTCTCTATGTTGCGGTGGATTA
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=148,22
PRIMER_LEFT_1_TM=59.056
PRIMER_RIGHT_1_TM=59.248
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=148
PRIMER_PAIR_PENALTY_2=1.7637
PRIMER_LEFT_2_PENALTY=1.011619
PRIMER_RIGHT_2_PENALTY=0.752117
PRIMER_LEFT_2_SEQUENCE=AGATCCGTCTCTGTTTGGTATGA
PRIMER_RIGHT_2_SEQUENCE=CTCTCTATGTTGCGGTGGATTA
PRIMER_LEFT_2=0,23
PRIMER_RIGHT_2=148,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=59.248
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=1.7982
PRIMER_LEFT_3_PENALTY=1.046061
PRIMER_RIGHT_3_PENALTY=0.752117
PRIMER_LEFT_3_SEQUENCE=ATCCCAAATTCACAAGGAACC
PRIMER_RIGHT_3_SEQUENCE=CTCTCTATGTTGCGGTGGATTA
PRIMER_LEFT_3=42,21
PRIMER_RIGHT_3=148,22
PRIMER_LEFT_3_TM=60.046
PRIMER_RIGHT_3_TM=59.248
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=1.8054
PRIMER_LEFT_4_PENALTY=0.513831
PRIMER_RIGHT_4_PENALTY=1.291527
PRIMER_LEFT_4_SEQUENCE=AAATTCACAAGGAACCAGCAAG
PRIMER_RIGHT_4_SEQUENCE=TCTCTCTATGTTGCGGTGGAT
PRIMER_LEFT_4=47,22
PRIMER_RIGHT_4=149,21
PRIMER_LEFT_4_TM=60.514
PRIMER_RIGHT_4_TM=59.708
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_01178 Ps_contig_mira-and-tgicl-ass_franssen_10841
SEQUENCE=AGTACGCGGGGATCTCCAAATCTCTCTCCACACAAAAGAAACTCTAACCCTTTTCTCTCTCTCTCTCTCTCAAATGGAGTCTCTCTCTTTAATTTATGTAGTTCCAGCATTGTACTTGTGTTTCCTATTATGTAAACTATTGGATCAAAAGAGGGACCAAGAATGTTACATATTAGATTACCAATGTCACAAACCAACACAAGATAGAATGTTAGGGACGAGAGTTTTGTTGGCAAAATAAATACAAAAACGAAACGTGTAAGG
TARGET=54,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1796
PRIMER_LEFT_PENALTY=0.156061
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=TCTCCAAATCTCTCTCCACACA
PRIMER_RIGHT_SEQUENCE=ACATTCTTGGTCCCTCTTTTGA
PRIMER_LEFT=12,22
PRIMER_RIGHT=165,22
PRIMER_LEFT_TM=59.844
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.3124
PRIMER_LEFT_1_PENALTY=0.288804
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=TCCAAATCTCTCTCCACACAAA
PRIMER_RIGHT_1_SEQUENCE=ACATTCTTGGTCCCTCTTTTGA
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=165,22
PRIMER_LEFT_1_TM=59.711
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.6165
PRIMER_LEFT_2_PENALTY=0.156061
PRIMER_RIGHT_2_PENALTY=0.460467
PRIMER_LEFT_2_SEQUENCE=TCTCCAAATCTCTCTCCACACA
PRIMER_RIGHT_2_SEQUENCE=GGAAACACAAGTACAATGCTGG
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=124,22
PRIMER_LEFT_2_TM=59.844
PRIMER_RIGHT_2_TM=59.540
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=0.6566
PRIMER_LEFT_3_PENALTY=0.156061
PRIMER_RIGHT_3_PENALTY=0.500551
PRIMER_LEFT_3_SEQUENCE=TCTCCAAATCTCTCTCCACACA
PRIMER_RIGHT_3_SEQUENCE=AACATTCTTGGTCCCTCTTTTG
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=166,22
PRIMER_LEFT_3_TM=59.844
PRIMER_RIGHT_3_TM=59.499
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=0.6615
PRIMER_LEFT_4_PENALTY=0.156061
PRIMER_RIGHT_4_PENALTY=0.505477
PRIMER_LEFT_4_SEQUENCE=TCTCCAAATCTCTCTCCACACA
PRIMER_RIGHT_4_SEQUENCE=CCAACAAAACTCTCGTCCCTAA
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=232,22
PRIMER_LEFT_4_TM=59.844
PRIMER_RIGHT_4_TM=60.505
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_01179 Ps_contig_mira-and-tgicl-ass_franssen_10879
SEQUENCE=GTAGTAGTTCAAGGGAAAGGAGGGTGTCGATAACACAGTATAAGTCATTGTGTTACAACAGGTCCAACATAGGTAGAAGTCCTATGGTAGAGTGTTGTGTTTGTTTGTCTGGTTTTGAAGAGAATCAGGAGGTGAGTGAGTTGCCTTGCAAGCATTTCTTCATAGAGGTTGTTTGGATAATGGTTGATAATAAACATAGTTCATGTCCTTATGTCGTTCAATGGAATAGTTAGTTAGTTGAGGTTAATTAGTNTTAATTGTTAGTAGTTAACTTGTTGTTAGTTTAAAGAAGAAGTTAATGCAGTTGGTTCTTATTTACTTTGTTTGTAGAA
TARGET=227,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01180 Ps_contig_mira-and-tgicl-ass_franssen_10906
SEQUENCE=TCGGGGAAATTCAAATTTAGCAGTAACTAACTACTTGTACCATAAATAAATAATGCTATTAACTCGATTCAKGACATCAATCTCTCATTTCAATTCCAAATCCAATTTTCTTCTCCTTTCCTCCGCTACTATGTCTTCCACCGCCGCCGTCGACGACGCCAAACACGGTTTCTCCCGTCCTGAGATGTACAAACAAATCTCGCGGAACCGTCGATTCCTACGATCGCCACGTGTTCNCTGTACAGA
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01181 Ps_contig_mira-and-tgicl-ass_franssen_10909
SEQUENCE=CCTTATAATAACAAAACAACATTAAATCCCTTGCACTTTGTTACAACTATTATTTCTCATCACAAGAAAACATTATTATTAGCAAAACTGAAAATAAATAAAAAAACAATCTCATGATTGCTACTATTGCAATCTAAAACAGCATTTATTTATCTGATCTATATTTATAATAAAGACCAGAAGAATAGAAAGCAACACATTTATTTACTCATGGATAAAAGAAGCCAATCGTTTGATCAATTTCTGACCATTCACAGATTCAGGATAAAAGATATGATAAACATGATCTTCGCCTTCTTCTTCAAACAATTCCAATTTCCCTTTCCAACCACTCTTTTTCACTATTTCATAATACCAAACTCCTCTCTCTCTAATTCCATCTTTCCCAGCAACACAAACAATCATCTTATCACAACCAAGTCCTTCCAAACTCGGTGCCTCTAAACCCAAAGGATTAACACAACAGTTATCAATTCCACCCGGTGCTGATGGATACACGAAATTCCAAACGGAGCAGTAAAAATCATGATCTGAACTCTTGAGTTTAACACCTTCTAATCCAACAGGGTATGAACTAAAGAAAAAAGGCTGTTGTAAAATAGCTCCTAAGAGTTTAACATCGTTCGGTAAAGCTTCAGAGCCAGCACGCATGGCGATGTTATGAACTATGTTACCACCAGCACTGTCACCACCAATGAAAAGTCTATTTAAATCACCGTGGTTGGTTAGCCACGGTTCTTGGTTAGAAGCAACCCATTGGAGTGAGCTCCAGCAATCATGGTACGCTGCAGGAAGAGGGTGTTCTGGAGCGAGTCTGTATTCGACGGAAACAACTATGGTGTTTGCTTGAGGAGCGAAGATGTTGAAGTGCTCATGGTAGAGTTTGGAGAAAGCTGATTCGAAAGAAGAATCCCACCCGGCCGGTAGGGAAAGAAATAAGTAAGNGTGGGGTAGTTTTTGGGGTGGGGTTGTTTGGGAGGTAG
TARGET=363,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0676
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.033470
PRIMER_LEFT_SEQUENCE=GATCTTCGCCTTCTTCTTCAAA
PRIMER_RIGHT_SEQUENCE=GAATTTCGTGTATCCATCAGCA
PRIMER_LEFT=284,22
PRIMER_RIGHT=504,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.967
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.0704
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.036271
PRIMER_LEFT_1_SEQUENCE=GATCTTCGCCTTCTTCTTCAAA
PRIMER_RIGHT_1_SEQUENCE=TAATCCTTTGGGTTTAGAGGCA
PRIMER_LEFT_1=284,22
PRIMER_RIGHT_1=456,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.964
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.0714
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.037339
PRIMER_LEFT_2_SEQUENCE=GATCTTCGCCTTCTTCTTCAAA
PRIMER_RIGHT_2_SEQUENCE=AGATGATTGTTTGTGTTGCTGG
PRIMER_LEFT_2=284,22
PRIMER_RIGHT_2=406,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.1445
PRIMER_LEFT_3_PENALTY=0.111077
PRIMER_RIGHT_3_PENALTY=0.033470
PRIMER_LEFT_3_SEQUENCE=CTGACCATTCACAGATTCAGGA
PRIMER_RIGHT_3_SEQUENCE=GAATTTCGTGTATCCATCAGCA
PRIMER_LEFT_3=243,22
PRIMER_RIGHT_3=504,22
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=59.967
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.1473
PRIMER_LEFT_4_PENALTY=0.111077
PRIMER_RIGHT_4_PENALTY=0.036271
PRIMER_LEFT_4_SEQUENCE=TCTGACCATTCACAGATTCAGG
PRIMER_RIGHT_4_SEQUENCE=TAATCCTTTGGGTTTAGAGGCA
PRIMER_LEFT_4=242,22
PRIMER_RIGHT_4=456,22
PRIMER_LEFT_4_TM=60.111
PRIMER_RIGHT_4_TM=59.964
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_01182 Ps_contig_mira-and-tgicl-ass_franssen_10931
SEQUENCE=GTGAAAGTATGAGCACAGAAAAATTAGGATGATTAGCAGTACAGTTACATTAGTTTTTTCTTTTGGTGAAATACATACTTAGTTCTTATTATACAACAAGATACATTTTTTGACTATTACTTGAGAACAATTACTGAGAGAGAGAGGGGAATGGGCGGGAGTGCATTCATGCAAGCTTCCTTTTGAAGTAATGGTATTTTTGGAGAGACTATTGAGTCTTGAAGAACCGACANAATAAGCCAAAGCAACGGAAAGGAGAGAATAGATCAACTAAGTTAAAAGGAAAGGTTGGGTNTTAGTAAAG
TARGET=136,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01183 Ps_contig_mira-and-tgicl-ass_franssen_10932
SEQUENCE=CTATCGTCGAACAAGCTTCACCTTGAAGCTTGACACAACAAGATATAAACCTTACTCTACACCCTACTCTAATCAACTACAACCATACCACAAATTCTCCCTACAAATTATAGACTCAAGAAGAAGAAGAAGANAGAAGAAGAAGAAGAAGAA
TARGET=118,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01184 Ps_contig_mira-and-tgicl-ass_franssen_10932
SEQUENCE=CTATCGTCGAACAAGCTTCACCTTGAAGCTTGACACAACAAGATATAAACCTTACTCTACACCCTACTCTAATCAACTACAACCATACCACAAATTCTCCCTACAAATTATAGACTCAAGAAGAAGAAGAAGANAGAAGAAGAAGAAGAAGAA
TARGET=135,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01185 Ps_contig_mira-and-tgicl-ass_franssen_10933
SEQUENCE=TAAATCCTAAAAATGGAATTAAACGCAAACGAAGGGGAACCAAGCCATCCAACCTTGGGGCTTTTATTCCCCCCATCCTACTCTACATGGCGGTAACATGATTGGAGCAAAGAAGCAGAGTTTTTGAAGACCTACCTACAAGACGGAGTCTGGCCTTGATTCTTGTTGAAGATGAGGATGATGAAGCTGAAGAATAAAAGAAGAGCAGAGAATACAATCATCAGATAAGTAATTTGAATTCTCAGTGCTTGCAGAATGTTTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTGAGTCTATAATTGAGGGANAATTTGNGGTATGGTTGTAGTTGATTAGAGTAGGGTG
TARGET=264,30
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=5.2979
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=5.272293
PRIMER_LEFT_SEQUENCE=GATTGGAGCAAAGAAGCAGAGT
PRIMER_RIGHT_SEQUENCE=CTCTAATCAACTACAACCATACCNC
PRIMER_LEFT=99,22
PRIMER_RIGHT=342,25
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=57.728
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=9.2250
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.75
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=5.3316
PRIMER_LEFT_1_PENALTY=0.059316
PRIMER_RIGHT_1_PENALTY=5.272293
PRIMER_LEFT_1_SEQUENCE=TTCTCAGTGCTTGCAGAATGTT
PRIMER_RIGHT_1_SEQUENCE=CTCTAATCAACTACAACCATACCNC
PRIMER_LEFT_1=238,22
PRIMER_RIGHT_1=342,25
PRIMER_LEFT_1_TM=60.059
PRIMER_RIGHT_1_TM=57.728
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.2250
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=5.3316
PRIMER_LEFT_2_PENALTY=0.059316
PRIMER_RIGHT_2_PENALTY=5.272293
PRIMER_LEFT_2_SEQUENCE=CTCAGTGCTTGCAGAATGTTTC
PRIMER_RIGHT_2_SEQUENCE=CTCTAATCAACTACAACCATACCNC
PRIMER_LEFT_2=240,22
PRIMER_RIGHT_2=342,25
PRIMER_LEFT_2_TM=60.059
PRIMER_RIGHT_2_TM=57.728
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.2250
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=5.5153
PRIMER_LEFT_3_PENALTY=0.243031
PRIMER_RIGHT_3_PENALTY=5.272293
PRIMER_LEFT_3_SEQUENCE=GAGTCTGGCCTTGATTCTTGTT
PRIMER_RIGHT_3_SEQUENCE=CTCTAATCAACTACAACCATACCNC
PRIMER_LEFT_3=145,22
PRIMER_RIGHT_3=342,25
PRIMER_LEFT_3_TM=59.757
PRIMER_RIGHT_3_TM=57.728
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.2250
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=5.5366
PRIMER_LEFT_4_PENALTY=0.264300
PRIMER_RIGHT_4_PENALTY=5.272293
PRIMER_LEFT_4_SEQUENCE=CTACATGGCGGTAACATGATTG
PRIMER_RIGHT_4_SEQUENCE=CTCTAATCAACTACAACCATACCNC
PRIMER_LEFT_4=82,22
PRIMER_RIGHT_4=342,25
PRIMER_LEFT_4_TM=60.264
PRIMER_RIGHT_4_TM=57.728
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.2250
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_4=261
=
PRIMER_SEQUENCE_ID=SSR_01186 Ps_contig_mira-and-tgicl-ass_franssen_10937
SEQUENCE=CAACAACAACAATAACATCAACACCCTCCTTAAAATTMSGGTTTCAAACACATTTCTCTGTCGAGTCATTTCAATTCAAAAAATCTCAAAATGAGTTCTCTAAATTTCTTTCTATTTTCATTCATCTTAGTTTCTTCACTCAAAATAGCAGAATCAAAAACACATTGGAAAGACATAGAGGCTCTTAAACAACTCAAACAGAACATAAACCTTAATTCAATGAATCCAGGCTCATGCGTGAGTACCTGGGACTTCACCTTCGACCCATGCGAAAATCTCTTCAGCGAGAAATTCACCTGCGGATTCAGATGCGACGTCGTCGTTTCACAGCTGAGTCGAGTCACCGAACTCGGTCTCGACCAAGCTGGTTACTCCGGTTCACTCTCTTCCATCGCTTGGGAGAATCTTCCTTATCTCGAAACTCTCGATTTCTCAAACAACTACTTCACGGGTCTCATTCCTGATTCTTTCTCAAACTTAACTCGTCTGAGTCGACTCAGTCTATCATCCAACTCGTTCACCGGAGAAATACCATCTTCCATTGGAACATCTCTTGTAAATCTCCAAGAGCTATATCTCGACAACAACAATCTCCAAGGAACAATCCCTTCAAGCTTCAACAATCTCAAAAGCTTAACAAGGCTCGAACTTCAACGCAATAACCTCACCGGAAAAATCACTGATTTGGGCTCTTTGGTGAATCTCTACCACATGGATCTCAGTGACAATCGTTTCTCCGAAAACCCATCTTCATTCCCGGTTTCTTTAGTTCAGATTTCAATACGGAACAACAATATAAGCGGTGGTTTAGCCTCTGAAACCTTCAAGAACATGATGTATCTCCAGGTAGTTGATTTCAGCTCCAACAAAATCAACGGCTATGTTCCTTCTATATTCTTCAACCTTCCATCGTTACAACAGTTAACACTTTCATTCAACGAATTTTCCTCCATTGAAGCCCCATACGGAACACAATCACTACAAAGCGAACTCATAGCAGTTGATTTGAGTAACAATCAGCTTCAAGGCTTGTTACCTGCATTTCTAGCGTTTTTACCTAGGCTTTCTTCTCTGTCTCTGGAGAATAACCGGTTCACTGGTTTGATACCGGTTCAGTTTGCTTTAAAGACGGTTTTTCCTGAAACCGGAATGGCTCCGTTTGGGAGGCTTTTGTTAGGGGGAAATTATTTGTTGGGAGGGATACCTCGTCCTTTGTTGGTTCTTAAACAAGATTCTGCTAATGTGAGTTTAGTTGATAATTGTTTGTTTAGGTGTCCTCATGTTTTCTTTTTTTGTCAAGGTGGACAACAGAAATCATCCTCGGAATGTAGTCATGTGATTATTCCTTAGATGATGAATTTATTACGATCATGGTATTTTATTTCATACTCATACATGTACATTTTTTATTTGWWTTTGGTTTTGGTTTTTTTCTWWTGTTCTTTTGTAACAGTAATCAATTTGTTATTATATTATAAGTTACTAAAGT
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01187 Ps_contig_mira-and-tgicl-ass_franssen_10946
SEQUENCE=GACCAAAAAAAACAGCTTTGGAAATGCTTTGATAAATCAATGAGGCAACAACTATAAASGCACTGGCCTCTTCTTGTCCTTTTCTTTCTCSAACCGCCGACGCTTCTCTTCTTTCTTTCTCTCCCCTCACCATCATTCTTCTTCCACTTTTCACTCCTCTGTAAACATCCACATAACCCTTACCATACACCTCAAATAAAACCCGAACNCGTCACATCTCAATTTTTATANAAATAAAACCCCACACGTGG
TARGET=109,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01188 Ps_contig_mira-and-tgicl-ass_franssen_10948
SEQUENCE=GGGGGGGTGGGAGTGAGTGGTAGTTGTTAACTATTTACTTTTCAGAACCGTGCAAACAAAACGCCAAGTCGTTTAACACAACATTGCAGATGCAGTCAAAGCTCAACAAATTTTTCAAATCTTCCTCCGATTCCACACCTCCTCCTCCCGCCGCCGCCGTCGCCGCCGATGACGGTGACCATGATTTGGCCAACTGGAACTGGGAGAACAATCAACACCATATTATCAACACTTACACCCGAACTCGTCGGAACCCTAATCCAATTACTTCACCTCCCACCTTAATCGAAAAACCGATAGTAGTCAAGAACAAGAAGAGAAGCTACGCTCAGTTCCATTTGGAGTTAGGTCAATCGGATTTCCTATTACGCGCGTGTTCCACTTGTGGTATTGAGTTCACTCCCGGAGATGTCGAAGACGAGAAATTACACGCCCAATTTCACAAACGTTATACGCAAGGAATCCAATTTAGAGGTTGGAATGATGAGAGGGTTGTTTCTTCTGACAAAAG
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2240
PRIMER_LEFT_PENALTY=0.059316
PRIMER_RIGHT_PENALTY=0.164639
PRIMER_LEFT_SEQUENCE=AGATGCAGTCAAAGCTCAACAA
PRIMER_RIGHT_SEQUENCE=ATCCGATTGACCTAACTCCAAA
PRIMER_LEFT=87,22
PRIMER_RIGHT=358,22
PRIMER_LEFT_TM=60.059
PRIMER_RIGHT_TM=59.835
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.2240
PRIMER_LEFT_1_PENALTY=0.059316
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=AGATGCAGTCAAAGCTCAACAA
PRIMER_RIGHT_1_SEQUENCE=TCCGATTGACCTAACTCCAAAT
PRIMER_LEFT_1=87,22
PRIMER_RIGHT_1=357,22
PRIMER_LEFT_1_TM=60.059
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=0.2240
PRIMER_LEFT_2_PENALTY=0.059316
PRIMER_RIGHT_2_PENALTY=0.164639
PRIMER_LEFT_2_SEQUENCE=AGATGCAGTCAAAGCTCAACAA
PRIMER_RIGHT_2_SEQUENCE=AATCCGATTGACCTAACTCCAA
PRIMER_LEFT_2=87,22
PRIMER_RIGHT_2=359,22
PRIMER_LEFT_2_TM=60.059
PRIMER_RIGHT_2_TM=59.835
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.2240
PRIMER_LEFT_3_PENALTY=0.059316
PRIMER_RIGHT_3_PENALTY=0.164639
PRIMER_LEFT_3_SEQUENCE=AGATGCAGTCAAAGCTCAACAA
PRIMER_RIGHT_3_SEQUENCE=AAATCCGATTGACCTAACTCCA
PRIMER_LEFT_3=87,22
PRIMER_RIGHT_3=360,22
PRIMER_LEFT_3_TM=60.059
PRIMER_RIGHT_3_TM=59.835
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.2867
PRIMER_LEFT_4_PENALTY=0.059316
PRIMER_RIGHT_4_PENALTY=0.227356
PRIMER_LEFT_4_SEQUENCE=AGATGCAGTCAAAGCTCAACAA
PRIMER_RIGHT_4_SEQUENCE=TATGGTGTTGATTGTTCTCCCA
PRIMER_LEFT_4=87,22
PRIMER_RIGHT_4=221,22
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=60.227
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=135
=
PRIMER_SEQUENCE_ID=SSR_01189 Ps_contig_mira-and-tgicl-ass_franssen_10958
SEQUENCE=GGCAGATTAGAATTTTGCAGTTTCTTTCTTTCCATTCTTCTTCTTCTTCTTGCATTTGCGTTGTTCATCTTTCTCAAAACCCTAACTCTATAAACACTTTCAGTACACATGTCAATTCACCCTCTTATGAATTTGCGATAACAACATGAACACTCTCTGCTRCCCCAACAACAATTCCTATCACATTCACACTCACCGCCACACAACACTTCATTCCAATCGCACCAGAACGCGGCAACTTCTTCTTCGGACGGCCAT
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01190 Ps_contig_mira-and-tgicl-ass_franssen_10958
SEQUENCE=GGCAGATTAGAATTTTGCAGTTTCTTTCTTTCCATTCTTCTTCTTCTTCTTGCATTTGCGTTGTTCATCTTTCTCAAAACCCTAACTCTATAAACACTTTCAGTACACATGTCAATTCACCCTCTTATGAATTTGCGATAACAACATGAACACTCTCTGCTRCCCCAACAACAATTCCTATCACATTCACACTCACCGCCACACAACACTTCATTCCAATCGCACCAGAACGCGGCAACTTCTTCTTCGGACGGCCAT
TARGET=35,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01191 Ps_contig_mira-and-tgicl-ass_franssen_10966
SEQUENCE=AAATNAGGANCTTGAGTTGTCATGTAACCTTGTTTATCTCCTCTTTGTTATAATTGTAATTCTCCAGTGGCTCCCATTTGGCGGCGGCCAGGCGCACATCAAAACACTTGATTTCCTTTCCTGATGAGACATAGATATTATCTTCATTCCCTGACTTGAAACACAAGGAAAGAATTGACTCGTCACTAACATCCATAGTGAGCCGCGGTTCATCATTGCATCGTAAATCAAACCAGCAAACACGACCATCCTCGCCGGAAGTGACAATAAGACGAGGGTTTTGAGAAGAGGCAATGCAGCAGTTAGTGCTGTCTTTGTGACCTTTCAATCGTCGAGCCTTCAGTTTAATCTTTGGAGGCTGGATATTAAGCGCACGCTGAGCTTCCTGCGCCACCGAAACCATTTCCGCTTCTCTGAAACCTAAATTCGCTGTATCTATAGGTAATTTTTAAGGAAAAGGAAAGAACGAATGTGGTGGTGGTGGTGTTGAGTTTGGAATTTGGATGGAATCAGGCGCTATCGAAGTTGCTGCAATGAAACCGCGCAACGCCGCAATGACACCGGGCACTGCCAAACACSACTGGTCCC
TARGET=472,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01192 Ps_contig_mira-and-tgicl-ass_franssen_10973
SEQUENCE=CCTTCTGATTTCGTAGGATGAAAATAGTGCAATCTGATGATGAAGATGATGATGATTCCGGAGACGAAGATGGCGCGAGACATGCTAGGATGTTACAAGGAATCACTGGGAAGACTATGATCAAGGATACTGGTA
TARGET=45,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5577
PRIMER_LEFT_PENALTY=1.672265
PRIMER_RIGHT_PENALTY=0.885426
PRIMER_LEFT_SEQUENCE=CCTTCTGATTTCGTAGGATGAA
PRIMER_RIGHT_SEQUENCE=AGTCTTCCCAGTGATTCCTTGT
PRIMER_LEFT=0,22
PRIMER_RIGHT=115,22
PRIMER_LEFT_TM=58.328
PRIMER_RIGHT_TM=59.115
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=2.6772
PRIMER_LEFT_1_PENALTY=1.672265
PRIMER_RIGHT_1_PENALTY=1.004943
PRIMER_LEFT_1_SEQUENCE=CCTTCTGATTTCGTAGGATGAA
PRIMER_RIGHT_1_SEQUENCE=CCTTGATCATAGTCTTCCCAGTG
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=125,23
PRIMER_LEFT_1_TM=58.328
PRIMER_RIGHT_1_TM=60.005
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=2.9073
PRIMER_LEFT_2_PENALTY=1.672265
PRIMER_RIGHT_2_PENALTY=1.235024
PRIMER_LEFT_2_SEQUENCE=CCTTCTGATTTCGTAGGATGAA
PRIMER_RIGHT_2_SEQUENCE=TCCCAGTGATTCCTTGTAACATC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=110,23
PRIMER_LEFT_2_TM=58.328
PRIMER_RIGHT_2_TM=60.235
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=3.1595
PRIMER_LEFT_3_PENALTY=1.672265
PRIMER_RIGHT_3_PENALTY=1.487218
PRIMER_LEFT_3_SEQUENCE=CCTTCTGATTTCGTAGGATGAA
PRIMER_RIGHT_3_SEQUENCE=TCTTCCCAGTGATTCCTTGTAAC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=113,23
PRIMER_LEFT_3_TM=58.328
PRIMER_RIGHT_3_TM=59.513
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=3.2360
PRIMER_LEFT_4_PENALTY=1.672265
PRIMER_RIGHT_4_PENALTY=1.563720
PRIMER_LEFT_4_SEQUENCE=CCTTCTGATTTCGTAGGATGAA
PRIMER_RIGHT_4_SEQUENCE=CCCAGTGATTCCTTGTAACATC
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=109,22
PRIMER_LEFT_4_TM=58.328
PRIMER_RIGHT_4_TM=58.436
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_01193 Ps_contig_mira-and-tgicl-ass_franssen_10974
SEQUENCE=AGTCGGGGAGCGGCTCAAGTCTGCCTCCATGAGTTAAGTGCTTCAGCTTCATCAAACATGGCAGACAAGAAGCGGAAGGAGAGGGGCGACGCCCAACGCAGCAGCCGCAACACCGTTTCTAAGAAGTCGAAGAAGACTTCCGACGTTCGCCGGAAAAGAACCGGTCCCCATTTGCCTTCATCTTTGAAAAAACAAATCGAAAGTTTAAACCCTACCACTGTTGATATCGATGAAGTTGATAATGACGTATACGAATACGAAGAAGAATTACCTGAAGAAGAATCGAGGAAGAACAAGCGTTACRACCCTGTCTCCGTCRAAGATAATGACCTTTCTTCTGATTTCGAGGATGAAAATGTGCAATCTGATGATGAAGATGATGATGATTCCGGAGACGAAGATGGCGCGAG
TARGET=376,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01194 Ps_contig_mira-and-tgicl-ass_franssen_10985
SEQUENCE=GTATTTGCTATAGATTTTATCTTAATTATTTTTTTCTTGAATTGTGTTTTTATGTGTCAAAGCAGCTAGATGATTGGGTCTTATGTAGAATCTACAAGAAGAAGAACACCACAAAATCATTGGAGACAAACCATGATTACTCACCAAACCAAATGAACATGACACAAAGAAAAGATGATAATGAACAAGAATTGGTGAAATTTCCAAGGACATGTTCCCTTACTAATCTTTTGGACATGGATTACATGGGACCAATATCACAAATATTATCAGATGGATCCTATAATTCAACTTTTGAATATCAAATGAATAGTGCAAGTGATGGAATAATTGACCCCTTTGTAAAGCCACAAATGGTTGAAATGGATACTACTAACAACCCTTATGGGGCTGATTCAGGGAAGTACCGTGGGAACCAAGTATGTGATTAGAGAGAATAACAACAAAAGAATAGATAATGACATTAATGGATTTTTAGGTATGACGATAAGGATTACCAATATAATAGTCATGAAGATTCTTTGTAGAAATATATATATATATATTACATCACACAATGAATGAATTTTCAGTTATAATCHMACTGATAGGTCCCTCAGAATATTTG
TARGET=530,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01195 Ps_contig_mira-and-tgicl-ass_franssen_10989
SEQUENCE=AACCATATAAGAAAGTGGGTTTGATGACAATTTAGATTTGTTATTGTCAACAACATCAAGAGATTGTGTCTTGTGGGTATATACCAAAGAAGAAGTTGAAGGGGTGGGTTTGATCATGGTAGTAGCTGATTGTTGTTGTTGCTGTTGATTATGAGGATGATCTTCAGTGTTTGGYGTTTGGAGAGGAAG
TARGET=130,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01196 Ps_contig_mira-and-tgicl-ass_franssen_11001
SEQUENCE=GGACATAGCCTGCATTTAAATACTGATTTTATTTGTACGACAAAAGCGGACTGTATATCTGCTGGTAGGGGTTTCTTTTCAGGCATAACATTAGATTTCTCAGAGTCACGTTCACTAGCAAACTTCTTGTTAATAAACACTTCCCTTTCTTTTATAGTTCCAGTATCATCTTCACTAAATAGCAGACCTGCAGAGTTTGTATCAGCAGAGTGCGCATCAATGTCGTTGGCAGAGCTATCCTCACTTTTATATCCAGAAGATGCTGAGCTAACTTCATCATCATCATCATTCTCCTGGCTTCCTTCCGCGGGTTGATTATCATAATCTTCATCCGAGGATGAAGAAGAATGATCTGCACCATCCCTATCTCCATGATCAACCTTAAAGATCTTCTCTTATNATTGTTTCG
TARGET=274,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0751
PRIMER_LEFT_PENALTY=0.036271
PRIMER_RIGHT_PENALTY=0.038821
PRIMER_LEFT_SEQUENCE=TAGGGGTTTCTTTTCAGGCATA
PRIMER_RIGHT_SEQUENCE=GATCATTCTTCTTCATCCTCGG
PRIMER_LEFT=65,22
PRIMER_RIGHT=352,22
PRIMER_LEFT_TM=59.964
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.0962
PRIMER_LEFT_1_PENALTY=0.057411
PRIMER_RIGHT_1_PENALTY=0.038821
PRIMER_LEFT_1_SEQUENCE=ACCTGCAGAGTTTGTATCAGCA
PRIMER_RIGHT_1_SEQUENCE=GATCATTCTTCTTCATCCTCGG
PRIMER_LEFT_1=185,22
PRIMER_RIGHT_1=352,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=60.039
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=168
PRIMER_PAIR_PENALTY_2=0.1200
PRIMER_LEFT_2_PENALTY=0.081185
PRIMER_RIGHT_2_PENALTY=0.038821
PRIMER_LEFT_2_SEQUENCE=GAGTTTGTATCAGCAGAGTGCG
PRIMER_RIGHT_2_SEQUENCE=GATCATTCTTCTTCATCCTCGG
PRIMER_LEFT_2=192,22
PRIMER_RIGHT_2=352,22
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.039
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=0.1255
PRIMER_LEFT_3_PENALTY=0.086698
PRIMER_RIGHT_3_PENALTY=0.038821
PRIMER_LEFT_3_SEQUENCE=GTTGGCAGAGCTATCCTCACTT
PRIMER_RIGHT_3_SEQUENCE=GATCATTCTTCTTCATCCTCGG
PRIMER_LEFT_3=224,22
PRIMER_RIGHT_3=352,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=60.039
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.2382
PRIMER_LEFT_4_PENALTY=0.057411
PRIMER_RIGHT_4_PENALTY=0.180795
PRIMER_LEFT_4_SEQUENCE=ACCTGCAGAGTTTGTATCAGCA
PRIMER_RIGHT_4_SEQUENCE=GATAGGGATGGTGCAGATCATT
PRIMER_LEFT_4=185,22
PRIMER_RIGHT_4=367,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=60.181
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_01197 Ps_contig_mira-and-tgicl-ass_franssen_11002
SEQUENCE=CTATCCGAGGATGAAGAAGAATGATCTGCACCATCCCTATCTCCATGATCAACCTTAAAGAATCTTCTCTTTATCATTGTTTCGTCTCAGTATAAAAATTGATTGAATTTCARAAGTAAGAAACACGAGAACAAGTGAAGAAAACTGAAGATAGCGCAAAGTAAGTGCGAGGTAGAAGTATAGTAGTTCCAGTGTTACACGGAGAAGAAGAAGAAGTTGGGAACGACGGCGAGAAAMCAGTC
TARGET=203,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01198 Ps_contig_mira-and-tgicl-ass_franssen_11020
SEQUENCE=AGATGCTTTGGTTTTTTGTCTTCTGTTTAGTGTTTGTTTGTTTTGTTTCTGTCTTATCTTATGCTCTAAGAAGGCAATGTTGGGCAACTTGCCCCAATGTGTTGACAATGGGCGTGAGCCGACATCTGTAGCTTTTATGTCCATGGAGGAATCAATGCACCGGTATTTGGTGGGTCTTGTTCGTCTTCAACTGGAAACTATATCTACTTTGGTAGACGTACAGATATTATCTCCAAAACTTGTTTAAATGTTCCTTTAATCTCATGCCTATGTTAAGAAGGTTAAATTCTAATGGAAATTGGAGTAAGAGGAGTCATTGATACCCAATCAATCAATAGTTATTCAAAAGGTTCAGTAATGAATTTGTTCTGAGGTGTAATATGCCTATATTAGCAGGTCAGATTACTGCGCCAAATCCTACAATTCCTGGAGTGTTGCAAAACATGTTTCCGATGGCTCCTAGTCAGATGCAGCAGTTCAGTGCTCTCCCTATGATGCCAATTCAGGCTATGACACAACAGGCTACTCGACATGCTAGAAGGGTGTATGTTGGAGGACTCCCTCCGACAGCCAATGAGCAGTCTGTTGCTATTTTCTTCAGCCAGGTCATGGCTAAGATTGGAGGAAACACTGCTGGTCCAGGTGATGCTGTGGTCAATGTTTACATTAACCATGACAAAAAATTCGCCTTTGTTGAGATGAGGTCCGTTGAGGAAGCTAGCAATGCAATGGCTTTGGATGGGATTATTTTTGAGGGGGCACCTGTCAAAGTCAGGAGACCTACTGATTACAACCCTTCTCTAGCTGCTACTCTAGGCCCAAGCCAACCTAACCCAAACCTGAACCTTGGCGCTGTTGGTTTAACCCCTGGGTCAGCTGGTGGACTTGAAGGCCCTGATCGCATTTTTGTGGGTGGACTTCCATATTACTTCACAGAAACTCAGATCAGGGAGCTTTTAGAAACTTTTGGTCCTCTTCGGGGTTTCGATCTGGTGAAAGATAGAGAAACAGGAAATTCAAAGGGTTATGCATTTTGTGTTTACCAAGATCTTGCTGTTACTGATATTGCATGTGCAGCTCTGAATGGAATTAAGATGGGAGATAAGACTCTTACTGTTAGACGTGCTAATCAAGGGACAACCCAGCCTAAACCTGAACAAGAGAGCATTTTAATGCATGCACAACAGCAAATTGCTCTGCAGAAACTTATATTTCAACCAGCATTAGTAGCTACAAAGGTGGTGTGTTTAACCAACGCAGTTGCTCCTGACGAGCTCAAGGAAGATGAGGACTTTGAAGAAATTATCGATGATATGAGACAGGAATGCTCCAAATTTGGTACTTTGGTGAATGTTGTGATCCCTCGCCCACAACCAGATGGGGATCTATCTGGTGGAGTTGGAAAGGTGTTTTTGGAATATGTGGATATTGAGGGTGCTACAAAAGCCCGCACTGGATTGAATGGGAGAAAATTCGGTGGAAATGAAGTAATAGCCGTTTTCTATCAAGAGAATAAGTTTGCTCAGGGGGATTATGAAGGCTAATTGCTGTAATGTCACTTGGATTATATCTCAAATTTTCATGTATGTTGTGAGATTGTTCAATATAAGTTTCTTTATTTCTTGTACACCGAGGTGGGAGCGGAGAAACTGATAGCTTCTGTTCTTAACTTGGATTTTTTTTTCATTTGATTGATAAATAATACTAGTTGATAATTTGTAACTTGTCATATCTTATGCTCTCTATCTGTGATATCTTCATATTA
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01199 Ps_contig_mira-and-tgicl-ass_franssen_11020
SEQUENCE=AGATGCTTTGGTTTTTTGTCTTCTGTTTAGTGTTTGTTTGTTTTGTTTCTGTCTTATCTTATGCTCTAAGAAGGCAATGTTGGGCAACTTGCCCCAATGTGTTGACAATGGGCGTGAGCCGACATCTGTAGCTTTTATGTCCATGGAGGAATCAATGCACCGGTATTTGGTGGGTCTTGTTCGTCTTCAACTGGAAACTATATCTACTTTGGTAGACGTACAGATATTATCTCCAAAACTTGTTTAAATGTTCCTTTAATCTCATGCCTATGTTAAGAAGGTTAAATTCTAATGGAAATTGGAGTAAGAGGAGTCATTGATACCCAATCAATCAATAGTTATTCAAAAGGTTCAGTAATGAATTTGTTCTGAGGTGTAATATGCCTATATTAGCAGGTCAGATTACTGCGCCAAATCCTACAATTCCTGGAGTGTTGCAAAACATGTTTCCGATGGCTCCTAGTCAGATGCAGCAGTTCAGTGCTCTCCCTATGATGCCAATTCAGGCTATGACACAACAGGCTACTCGACATGCTAGAAGGGTGTATGTTGGAGGACTCCCTCCGACAGCCAATGAGCAGTCTGTTGCTATTTTCTTCAGCCAGGTCATGGCTAAGATTGGAGGAAACACTGCTGGTCCAGGTGATGCTGTGGTCAATGTTTACATTAACCATGACAAAAAATTCGCCTTTGTTGAGATGAGGTCCGTTGAGGAAGCTAGCAATGCAATGGCTTTGGATGGGATTATTTTTGAGGGGGCACCTGTCAAAGTCAGGAGACCTACTGATTACAACCCTTCTCTAGCTGCTACTCTAGGCCCAAGCCAACCTAACCCAAACCTGAACCTTGGCGCTGTTGGTTTAACCCCTGGGTCAGCTGGTGGACTTGAAGGCCCTGATCGCATTTTTGTGGGTGGACTTCCATATTACTTCACAGAAACTCAGATCAGGGAGCTTTTAGAAACTTTTGGTCCTCTTCGGGGTTTCGATCTGGTGAAAGATAGAGAAACAGGAAATTCAAAGGGTTATGCATTTTGTGTTTACCAAGATCTTGCTGTTACTGATATTGCATGTGCAGCTCTGAATGGAATTAAGATGGGAGATAAGACTCTTACTGTTAGACGTGCTAATCAAGGGACAACCCAGCCTAAACCTGAACAAGAGAGCATTTTAATGCATGCACAACAGCAAATTGCTCTGCAGAAACTTATATTTCAACCAGCATTAGTAGCTACAAAGGTGGTGTGTTTAACCAACGCAGTTGCTCCTGACGAGCTCAAGGAAGATGAGGACTTTGAAGAAATTATCGATGATATGAGACAGGAATGCTCCAAATTTGGTACTTTGGTGAATGTTGTGATCCCTCGCCCACAACCAGATGGGGATCTATCTGGTGGAGTTGGAAAGGTGTTTTTGGAATATGTGGATATTGAGGGTGCTACAAAAGCCCGCACTGGATTGAATGGGAGAAAATTCGGTGGAAATGAAGTAATAGCCGTTTTCTATCAAGAGAATAAGTTTGCTCAGGGGGATTATGAAGGCTAATTGCTGTAATGTCACTTGGATTATATCTCAAATTTTCATGTATGTTGTGAGATTGTTCAATATAAGTTTCTTTATTTCTTGTACACCGAGGTGGGAGCGGAGAAACTGATAGCTTCTGTTCTTAACTTGGATTTTTTTTTCATTTGATTGATAAATAATACTAGTTGATAATTTGTAACTTGTCATATCTTATGCTCTCTATCTGTGATATCTTCATATTA
TARGET=325,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2251
PRIMER_LEFT_PENALTY=0.151106
PRIMER_RIGHT_PENALTY=0.073989
PRIMER_LEFT_SEQUENCE=GTATTTGGTGGGTCTTGTTCGT
PRIMER_RIGHT_SEQUENCE=AACATGTTTTGCAACACTCCAG
PRIMER_LEFT=162,22
PRIMER_RIGHT=447,22
PRIMER_LEFT_TM=60.151
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.3216
PRIMER_LEFT_1_PENALTY=0.151106
PRIMER_RIGHT_1_PENALTY=0.170538
PRIMER_LEFT_1_SEQUENCE=GTATTTGGTGGGTCTTGTTCGT
PRIMER_RIGHT_1_SEQUENCE=CTCCAGGAATTGTAGGATTTGG
PRIMER_LEFT_1=162,22
PRIMER_RIGHT_1=431,22
PRIMER_LEFT_1_TM=60.151
PRIMER_RIGHT_1_TM=59.829
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.4949
PRIMER_LEFT_2_PENALTY=0.151106
PRIMER_RIGHT_2_PENALTY=0.343828
PRIMER_LEFT_2_SEQUENCE=GTATTTGGTGGGTCTTGTTCGT
PRIMER_RIGHT_2_SEQUENCE=GTAGGATTTGGCGCAGTAATCT
PRIMER_LEFT_2=162,22
PRIMER_RIGHT_2=420,22
PRIMER_LEFT_2_TM=60.151
PRIMER_RIGHT_2_TM=59.656
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.6772
PRIMER_LEFT_3_PENALTY=0.603210
PRIMER_RIGHT_3_PENALTY=0.073989
PRIMER_LEFT_3_SEQUENCE=CTTGTTCGTCTTCAACTGGAAA
PRIMER_RIGHT_3_SEQUENCE=AACATGTTTTGCAACACTCCAG
PRIMER_LEFT_3=175,22
PRIMER_RIGHT_3=447,22
PRIMER_LEFT_3_TM=59.397
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.6854
PRIMER_LEFT_4_PENALTY=0.151106
PRIMER_RIGHT_4_PENALTY=0.534252
PRIMER_LEFT_4_SEQUENCE=GTATTTGGTGGGTCTTGTTCGT
PRIMER_RIGHT_4_SEQUENCE=TTGCAACACTCCAGGAATTGTA
PRIMER_LEFT_4=162,22
PRIMER_RIGHT_4=439,22
PRIMER_LEFT_4_TM=60.151
PRIMER_RIGHT_4_TM=60.534
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_01200 Ps_contig_mira-and-tgicl-ass_franssen_11032
SEQUENCE=ATTCACAATCCCCACCATTCCAAAACAATGAAACCTTTTCTTTTCTTTTCTCATCCCATTCTCTTTCTTTTTCCAAACACAGTTACCACTAGTTTCTTCTGCTACAACTCTTCCCTTTCAACTCATCACTCTCCTTTCAATAAAATCAGCCCTCATTGACCCTCTTAATCATCTCAATGACTGGAAAATCAACCCTTCTAATTCTTCCTCTGCTTTCTCAAACTCCAATAACTCCCAAGACCCAATTYGGTTTGG
TARGET=35,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01201 Ps_contig_mira-and-tgicl-ass_franssen_11035
SEQUENCE=TAATACACAGAACTTAATCATCGGGAGGAGAGGGAAAAAAGGAACTGAGGAAACATCACAATCTCACAAGCAAAGTACACAACAGAAACCCTATAATCTCCAGATTTTGGTGCAAGAATTATACTCCCACTCCCTTGTATATTTATCTAGAATGTAGTTCTTTACAACAACTTCATTAGGGGGGTAACAAGTTACAGCATGTTTTGTTAAAACATCTTGGAGACTCAGCCAAATGGTGAAGAAACAGGTCCTGCAATAGCATCAGACCTTTTGATGCTTCCCATTCCACACACGATGCATCCAGGTGCAGTCACAGCCGAAAATTTGGCCCCACAGAGATCACACACATCATATCCAATGGTAGACAGCCTGCTTAGAGGTGGCAGCACAGAAATGTGAGGGATCTTCGTAAGGGATACAAGTAGGGTACTTTAGGTTTAAGGTACCAAACCGGCCCCCCTTCTAGAAACCAACCAACCGAGGGTACCAAACCCG
TARGET=468,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01202 Ps_contig_mira-and-tgicl-ass_franssen_11052
SEQUENCE=GGGGAAGCAATGTTCTCTCCGAAGGAATGTTCCACTTTCTCACAAGTGTTTTGTTTGTGCTAAGGTTTTTGCAGCATCCATGTCTTGTTTTTTAATTCTTTAGTGCATARTGTGATGATGATAGAATTAGAAAATGACATGCAATTAATTTAGTTTATGTACTTTTTCACTCTCTCTCTCAAACNCTATATCCTATGTTGNGGANCATCTGTT
TARGET=170,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01203 Ps_contig_mira-and-tgicl-ass_franssen_11059
SEQUENCE=TTGTCCTTATCCCCAGTTGGTGGTCCCTCCTTCAATTACTCTCCAATCTCCAAAAACTTCACCGTTGCAGCTTCTCTTCTATTGTTGGTGGTACATTGATGCAATTGGGGAGAACCAAACAGATTCTTTTGAACAACATCAGTGTAAATTGAACCACTCCCTGCTGTTGTGACATGGGTAGGATCAACCTCATATGGTCCACTTCTACTGCTACCAGGAACAGATGAAGACAAAGCCAAACGGTGGGAAGGAGAATGTAATGTGTTCCGTGGACGAGCGGAATCAATCATTGCACTGATGCATCTTGTACAAGGAGACAGACGACTTTGTCGATGTAAATGATCAGTTGTGGATTCAATGGTAGGAGTTTTCCTCACTTAAAATGTCACAGACCATCTTTCCAGTTTTAGWACAATTACATGTCACTTGCTGCTTGATTTCCCAAAGAGCCCGGCAAGCATGTGCGGAGAAGGCACGAGTCTGGCAGTCAACAACAACAAGTGTGTATGGTGTTGGATTGTTTTGGCTGA
TARGET=489,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01204 Ps_contig_mira-and-tgicl-ass_franssen_11066
SEQUENCE=TTATAGGAATTAGTTAGTTTCTTTACTAATATATGTCTATTCCTATATATATATATATGTCCACGCGTTTAATAAGAAATTATATTATTATCCTAATTTATTTGG
TARGET=44,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01205 Ps_contig_mira-and-tgicl-ass_franssen_11067
SEQUENCE=TTGGTTTATGAGGCTTTTCAAAATAAGAGATTTTGAAGATATGTTTGGCCAACTTTTCTTAGTAAGTTACGTCATGGCTCTTAGTTATCCAATTTTTGAAGGGATTTTAACTATGAAAAGTAAGAGTGGATAGTGGAACTTCTTCAAGAATTCAAAGTGCATAAGTATTTGTATCTTAAAATGAAATCCTCATAATTATGTTTAAATTTTTGTTTTGTTGTTGAGATAATTGTTGTGTTTAGATTAATAAAATAATTAGGTTTTTCTTCTCTTTYTTATAGGAATTAGTTAGTTTCTTTACTAATATATGTCTATTCCTATATATATATATRTGTCCACGCGACTTAAATAAGAAATTATATTAATTAATAAAAAACCG
TARGET=319,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01206 Ps_contig_mira-and-tgicl-ass_franssen_11068
SEQUENCE=CTCCGTTAAGTTCAGAGCCGGATCATAGCCAAGTCCCAAAACATGCCAACCCCGATCTAGCGGATAGGTCAGTTTATAAAAGGTGATTAAGCCTGGTGGTAATGTTCCAAGCTTCCAGAGGGTTCTATCCTCATTCAAATCTTGCCACCAATGATAGATTCCGGTGATGTTTCGTTTCTTCCACTCCTTCAAGTCAAACATATTCATGCCAAATGCCCAACCACATGCATCGGGACTGAAATTACTGGAGATCAGTGGATTACTAAAATTTAGGTATTTATCAAACCTATGGAAGCTCTCCTTGCATGTCTCTACTGCACCGATCACCATACCTTTCAAATCAATTGACCAAAGAGGTGCCAAATCTTTCTGCACGACAATGTCATCATCCAAGAATAGAATTTTGTTCTAATTTTGGATAAACTTCGGGAAGGTAGAACCTTAAGTGATTCAGCATTGACAAATATTTGGGATTTCTATATTTTAGATTGTCAGAACCAGCAGAGAGAGAGGAAGGATGATTGGCTTTAAAGTAATACTCCTTGATTCTTGCTGATTCAAGTTGACGTAAAACAGAACAATACGAGGAATTCAGCCACTTGAAATCATCAATATTCTGAACTTCAATGGTTGCTTTAGAAGGAGGGTTGATGAGAA
TARGET=503,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0500
PRIMER_LEFT_PENALTY=0.022302
PRIMER_RIGHT_PENALTY=0.027712
PRIMER_LEFT_SEQUENCE=TCTACTGCACCGATCACCATAC
PRIMER_RIGHT_SEQUENCE=GGCTGAATTCCTCGTATTGTTC
PRIMER_LEFT=310,22
PRIMER_RIGHT=596,22
PRIMER_LEFT_TM=60.022
PRIMER_RIGHT_TM=59.972
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.1020
PRIMER_LEFT_1_PENALTY=0.022302
PRIMER_RIGHT_1_PENALTY=0.079714
PRIMER_LEFT_1_SEQUENCE=TCTACTGCACCGATCACCATAC
PRIMER_RIGHT_1_SEQUENCE=ACGTCAACTTGAATCAGCAAGA
PRIMER_LEFT_1=310,22
PRIMER_RIGHT_1=568,22
PRIMER_LEFT_1_TM=60.022
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.1027
PRIMER_LEFT_2_PENALTY=0.075027
PRIMER_RIGHT_2_PENALTY=0.027712
PRIMER_LEFT_2_SEQUENCE=CCGATCACCATACCTTTCAAAT
PRIMER_RIGHT_2_SEQUENCE=GGCTGAATTCCTCGTATTGTTC
PRIMER_LEFT_2=319,22
PRIMER_RIGHT_2=596,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=59.972
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.1547
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.079714
PRIMER_LEFT_3_SEQUENCE=CCGATCACCATACCTTTCAAAT
PRIMER_RIGHT_3_SEQUENCE=ACGTCAACTTGAATCAGCAAGA
PRIMER_LEFT_3=319,22
PRIMER_RIGHT_3=568,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.1817
PRIMER_LEFT_4_PENALTY=0.154023
PRIMER_RIGHT_4_PENALTY=0.027712
PRIMER_LEFT_4_SEQUENCE=CTCTACTGCACCGATCACCATA
PRIMER_RIGHT_4_SEQUENCE=GGCTGAATTCCTCGTATTGTTC
PRIMER_LEFT_4=309,22
PRIMER_RIGHT_4=596,22
PRIMER_LEFT_4_TM=60.154
PRIMER_RIGHT_4_TM=59.972
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_01207 Ps_contig_mira-and-tgicl-ass_franssen_11071
SEQUENCE=AGAATATAGGGGAGTAATAATAATAGAATAATAACAATAAATATCCTCTTCGCTTAATAAAAAAATAAATAAATATCCTCTTTTATTTTTCACAAACATCTACAATAAATAATTAAATACAAAATAAATAGTGTAAAAATCTCTCACTCTCCACTATACATACATAGATGAACTCGTAGAAGTAATCAATCAGATCCATGTTCTGTTACAAAACGGTAATAAGAATCATGCATCGGAAAAGAATCAATCATGTTCCCACAAAATAGACTCATAGTGTTTTTCTCAGTTCTTGAAGATAGCTAGCATAGACAGGACAATTGAAGCTCCGATCATAGCCACAGAATTTGTCACAGATCCGGCAGCTCCGGCATCAGGGCCGGGCGCTGGAGCCAACGAGGGACTGAGATCTTGAGCGGAGGCAACGGCGGCGAAAACAACGACGAATAGGAAGGAAAAGATGTTGACAGTGGTAGCTGATGATGCCATTGTAAAAATTCTTAACAGAGAAGAAGAAAAAGCTGAATTGAAAAAAAAGTGGAAAATGTGAATTAAGGAGAAGCTTTTGTGTTGAAGAGATTGTGATTTCCCCGCGTACTCTGCGTTGA
TARGET=63,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01208 Ps_contig_mira-and-tgicl-ass_franssen_11090
SEQUENCE=ASGAATGGATGAATGGAGCATCCCATCTGGCAACAAGTGGTCCCATTTTAGTTTTCTCGGTCGGCGCTGGCTCGCTCATCCATTGTTTTATGTCATAGCAACCCTAGATCCCTAAATTTTACAATTCAATCTGAATCTAGATTCCTAATTATTCTCTTTCAATTCAATCCTATATGTATCTATTCCTTCCTCCAGGTAACTTTTTTCAATTCAAATCATCCAAGATAAGGTGGTTTTCTGGTTGACGTTTGCAGGTTTATTGCTTGTGCTGAAATGGTCTTTTGTAATTCTAGTTCTGCCAATGAAACCTATTGTATAATCCTTATTCTACATCTCCCGAAATGCAATTAGCTTCTAACTCTGAATCCCAACCCATCTTGAATCACTCCTGTGAAATTATTCCTGACAATGACGAGGAGGAGGAGGAGGATATAGAGAATGTTCGCCTTCACCACTCTTCTCATCTTCTTGTTACAGATCAGCCACAATGCCGAATATGCCTTGATTTTGAAGGAGAAGACTTAATTGCCCCCTGCCATTGCAAAGGTACACAAAAATATGTCCATAGATCATGTTTGGATAACTGGAGGTCTACCAAGGAGGGCTTTGCTTTTTCTCACTGTACAGAGTGCAGAGCTGTCTTCCTATTACGACCCAATGTCCCAAAAGATCGCTGGTGGTTGAGGTTGAAGTTTCAGTTCCTTGTTGCCAGAGATCATGCATTCATTTTCATCATTGTTCAACTGGTTGTTGCTTTCTTGGGGGTGCTTATTTACAAATTCTATGGGGATGAACTTAGAGAAATGTTTGGTTATGAAGAACATCCCTATGGATTTTATACAATGGCTGTTCTCGCCATTATTTTGGTGGGTTTGCTCTATGGCTTCTTTATTGCAATAATATGTGGCCAAAGAATCAATGAACGCCACTACCATGTTCTTGCCAAACAAGAGTTGACAAAGGAATATGTGGTAGAAGATCGAGAACATGTAAAGAATGTGCCCGAACTCGACCCCAGCCATGTGACAGAACTAAGGATGCTGGGCCTTTACTAGCCGGTATATGTATATCCCATTCTTTCCCGTTTAAATTTCCTGTCTTCTTCCACTTTTAGATGTTAAATTAGAAACAGTATAAAGAGAAAGGGAGACCAATCAAAAGTTTAGGCAAAACTGTTAAAAGGTATTTAGAATGAAGTGGTCTCTTACAAAATATTATCATTTCGTTTGATCCATGTAGCTTATCCACCAACTGGACTATCTTTTTTATTTCTCTACCACTTTGTATCTTGAATTCTATTGGTAGTGATATGATACTGTTTCATTTCAG
TARGET=414,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01209 Ps_contig_mira-and-tgicl-ass_franssen_11105
SEQUENCE=GTCGGGGTAGACTCTGATTCATTCACCAAAATCCGATCCTTCTCTCGAATTCTTGAACCTTTCTCTGAAGAAGATGACCGATTCTTTCGCTCACTGTGCGTCACACATTAATTACCGTCACAAAATGAAAACTATGTTTATCTTCTCCACACCGTGTTGTTCTCCTTCAACCGCGTTTTTCTCACCTTTTAGGGCTTCAAATTCTAAACCTCTTCGTTCTACTCTTTCTCTTCGCTCTTCAATTTCTTCTTCTTCTATAACTTCCACTTCTCATTGTTCACTCGCTTTCAACATTGTTAAACATAAAGAGAAAAACGTTGTTTCCGCGAACATGACCTCTTCCGTTTCTTCTCGCACTTTTCTCAACGCTCAAAATGAACAAGATGTTCTTTCTGGAATTAAGAAAGAAGTAGAAGCGGGAACTTTGCCTGCTAGTATTGCTGCAGGGATGGAAGAAGTGTACCTTAATTATAAAAGTGCAGTTATTAAAAGTGGAGATCCCAAAGCAAATGAAATTGTATTATCAAATATGACTGCCTTATTAGATCGCATATTTTTGGATGTGAAGGAGCCTTTTGTCTTTGAAGCACACCACAAAGCAAAGAGAGAGCCTTTTGACTACTACATGTTTGGTCAAAATTATATTCGTCCCTTAGTTGATTTCGAAACTTCTTATGTTGGTAACATGCCCCTTTTCATTCAAATGGAAGAACAACTTAAGCAGGGGCACAATATTATCTTGATGTCAAACCACCAAAGTGAAGCAGATCCAGCTATTATTGCATTGCTGCTTGAAATGCGACTTCCACATATTGCTGAAAACTTGATTTATGTAGCAGGAGATAGAGTTATAACCGATCCTCTATGCAAGCCCTTCAGTATTGGCAGGAATCTGATCTGTGTTTATTCGAAAAAGCACATGCTTGATAATCCAGAACTTGTAGATATGAAAAGAAAAGCAAATACAAGAAGTCTGAAGGAAATGGCTATGCTTTTAAGGAGTGGATCACAAATAATTTGGATTGCTCCAAGTGGTGGTAGGGATCGACCAGTTGCCAATTCTGGGGAATGGGCACCGGCACCCTTTGATTCTTCTTCAGTGGATAATATGCGAAGGCTTGTCGATCATTCAGGTCCACCAGGTCATATCTATCCTTTGGCAATATTGTGCCATGACATAATGCCTCCTCCACTAAAGGTCGAAAAAGAAATTGGGGAGAAAAGAATTATATCCTATCATGGGACTGGCATATCAACTGCTCCAGAAATAAGCTTTTCCAACACCACTGCTGCTTGTGAAAATCCTGAAAAGGCTAAGGATGCTTATACAAAAGCCTTGTATGATTCTGTGACCGAACAATATGATGTGCTGAAGTCTGCTATACACGGGAAAAAAGGATTACAAGCATCAACTCCTGTCGTTTCTTTGTCACAGCCATGGAAGTAGTTCAGCTCTGAATTTTTTGTTACAATTGTCTGATTATCAGATGCAAGTTTCAAGAAGTCAGTCAAACAATCCAAACCGATTAGCTTCAGAGATGACTGTCTTTTACTACATCCAATGATTAGCTTGATGGAAGTTTCCTGTCAATGATATTTCCTTTTCATTTTTCTGTTTGCGCTTGCAATTGTCAGGAATCTGTTTCTGAAGGTTCAAAATCAGCTGCTTACTGTACAGGTCTCAGTTCCATCTCACATCCAGTCCATTGACTGATAAGAGACCAATATAGTTTTTTACCCTTTTTTTATTATCAAGATTTCTTTTGGCTAGCTAAATAGATAATAGCTAATTTTCTCTGTGTAATTTTTAGTCAAATTTGTTCCATTATAGATGTATAATTGTTAATATTTAGAACCTCTTTCATATTCAAATTTATTCAAACGCGCCACAGTATAAGCAAGGTCAAAATGCAGTTGGAAGACTATTTGCGATGTCAGAATAAATAAAAGGGCCACATTTACGATAGMAAMMCT
TARGET=244,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01210 Ps_contig_mira-and-tgicl-ass_franssen_11106
SEQUENCE=CTCTATATTTGGCCAGTCGGGGGATCCTCCATGAAGACTCTTTATAATACACAAAAGATGGCTCCTACATTAGCATTTCACTTTCTTTCTTTGGTCCTTTTCTTCGTTACGATGGGTGAAGGAATCATTGTTGAAGACATGAAAATAGAGTTACCAGATCAAAAGGATATCGAGGAAGCTAAACAATCAAATCATCTGCATAACTTAATCGATGAAGCTAAAAAACCTAATTACAATTCTGAAGATATTACCCATGATCCCAATCCTTGGCTCCCTTGGGGTAGTCGTGAAACCAAAAAACCTAATTTACAATTCTGAAGTTAATACCCATGATCCTAATCCTTGGCTCCCTTGGGGTAGTCGTGAAAATCAAAAAAACCTATTTACAATTCTGAAGTTAATACCCGTGATCTTAATCCTTGGCTCCCTTGGGGTAGTCGGGAAACCAAAAGACCTATTTACAATTCTGAAGTTAATACCCGTGATCCTAATCCTTGGCTTCCTTGGGGTAGTCGTGAAACCATAAACCTATTTACAATAATGAAGTTAATACCCGTGATCTTAATCCTTGGCTCCTTGGGGTAGTCGTGAAATCAAAAGACCTATTTACAATTCTGAAGTTAATACCCGTGATCCTAATCCTTGGCTCCCTTGGGGTATCGTGAAAACCAAAGCCTTATTTACATT
TARGET=81,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2918
PRIMER_LEFT_PENALTY=0.257927
PRIMER_RIGHT_PENALTY=0.033905
PRIMER_LEFT_SEQUENCE=TGGCTCCTACATTAGCATTTCA
PRIMER_RIGHT_SEQUENCE=CCAAGGATTAGGATCATGGGTA
PRIMER_LEFT=58,22
PRIMER_RIGHT=345,22
PRIMER_LEFT_TM=59.742
PRIMER_RIGHT_TM=60.034
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.3337
PRIMER_LEFT_1_PENALTY=0.257927
PRIMER_RIGHT_1_PENALTY=0.075795
PRIMER_LEFT_1_SEQUENCE=TGGCTCCTACATTAGCATTTCA
PRIMER_RIGHT_1_SEQUENCE=CCTCGATATCCTTTTGATCTGG
PRIMER_LEFT_1=58,22
PRIMER_RIGHT_1=174,22
PRIMER_LEFT_1_TM=59.742
PRIMER_RIGHT_1_TM=59.924
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.8557
PRIMER_LEFT_2_PENALTY=0.257927
PRIMER_RIGHT_2_PENALTY=0.597751
PRIMER_LEFT_2_SEQUENCE=TGGCTCCTACATTAGCATTTCA
PRIMER_RIGHT_2_SEQUENCE=AGGATTGGGATCATGGGTAATA
PRIMER_LEFT_2=58,22
PRIMER_RIGHT_2=266,22
PRIMER_LEFT_2_TM=59.742
PRIMER_RIGHT_2_TM=59.402
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.8756
PRIMER_LEFT_3_PENALTY=0.257927
PRIMER_RIGHT_3_PENALTY=0.617670
PRIMER_LEFT_3_SEQUENCE=TGGCTCCTACATTAGCATTTCA
PRIMER_RIGHT_3_SEQUENCE=AAGGATTGGGATCATGGGTAAT
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=267,22
PRIMER_LEFT_3_TM=59.742
PRIMER_RIGHT_3_TM=60.618
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=0.9034
PRIMER_LEFT_4_PENALTY=0.257927
PRIMER_RIGHT_4_PENALTY=0.645442
PRIMER_LEFT_4_SEQUENCE=TGGCTCCTACATTAGCATTTCA
PRIMER_RIGHT_4_SEQUENCE=AGCTTCCTCGATATCCTTTTGA
PRIMER_LEFT_4=58,22
PRIMER_RIGHT_4=179,22
PRIMER_LEFT_4_TM=59.742
PRIMER_RIGHT_4_TM=59.355
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=122
=
PRIMER_SEQUENCE_ID=SSR_01211 Ps_contig_mira-and-tgicl-ass_franssen_11122
SEQUENCE=GACAAACTCAAATCCCATTKCTCACAAACTCAAATCATTCTCTTCTTCTTGATCTCTCTCTTCTTGGACGCCATTTTCGTTATCTCTCAAAATCTCTCTGCAAACCAGTGGATGGCAAAAAACAACAAATATGCTTCCATCAACTTCAACCACATCTACGACAAATCCATAACAACCACCACAAACTCATCGAAAAGCCCCTCCATTTCTTCATCTTCATCCTCCTTCTCCTCCGCTTCCTATTCTGCAGTTTCCGCTCCCAACAAACATGGCCGCATCCTCGTTCTAACACGACCCACACCGAAACCCATCGCACAACCTCCCACCCCTTCTCCCGCACCACCATCCAAACCAACCCCGATCTTACAGACTCCTCCAGATCGACCCCGGTCCGAACCGGGACCAGATTCGATCTCGCTTCGTCCTCTCGGTCGAACCGGTGTCGGGTCGCCTCTTTTACCAACCCCGAATCTGAATCACGAGAAAGAGAAAGATTTGCCTTCTCTGAAGCCCAATAAGTTTATCCCGCCGCATCTTCGACCGGGGTTTGTGCCCAGAGGGGAGCCTCCTAGACCGGAATTGGGTCGGTTTAGGGAAAACTTGCGACCCAAGTCTGGTGGAAACGAGAGGATGAGGAGGGTTGGTGTCTCAGATTCGAGTTTCCAGAATCGCCCCAGTTCTAGCGGATGATGATGATGATGATGATGAATAGTTGAGTTCTTTTCTGATATCTGAAATCATTGAAGATGAAGACAGATTCTCAACACCTAACTCTCACAATTTGTAATGAAAATTGTAGATTTTGACAAGATGATTTGCTTCTGATGATGACAATAGTGTAGAATTAGAATCTAAGTACATATTTTTAATTTCATGTTGAACTTTTTTTGTCTGTTTTTGTGGGCATGCCATTGTGATTTGAGCATGTGTTGTTTGTTGTATTTCATTCTTTTGGTAGTTGTTTTGCACAAGCTTGGTCTTTTCAGTTTACTAAAAAAA
TARGET=686,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01212 Ps_contig_mira-and-tgicl-ass_franssen_11123
SEQUENCE=CGCCGCATCTTCGACCGGGGTTTGTGCCCAGAGGGGAGCCTCCTAGACCGGAATTGGGTCGGTTTAGGGAAAACTTGCGACCCAAGTCTGGTGGAAACGAGAGGATGAGGAGGGTTGGTGTCTCAGATTCGAGTTTCCAGAATCCGCCCCAGTTCTAGCGGATGATGATGATGATGATGATGTTGAGTTTCTTTTCTGATATCTGAAATCATTGAAGATGAAGACAGATTCTCAACACCTAACTCTCACTAATTTGTAATGAAAATTGTAGATTTTGACAAGATGATTTGT
TARGET=161,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.1299
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=3.055462
PRIMER_LEFT_SEQUENCE=CAGATTCGAGTTTCCAGAATCC
PRIMER_RIGHT_SEQUENCE=GGTGTTGAGAATCTGTCTTCATCTT
PRIMER_LEFT=123,22
PRIMER_RIGHT=238,25
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=60.055
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=3.1729
PRIMER_LEFT_1_PENALTY=0.117452
PRIMER_RIGHT_1_PENALTY=3.055462
PRIMER_LEFT_1_SEQUENCE=GGTGTCTCAGATTCGAGTTTCC
PRIMER_RIGHT_1_SEQUENCE=GGTGTTGAGAATCTGTCTTCATCTT
PRIMER_LEFT_1=116,22
PRIMER_RIGHT_1=238,25
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=60.055
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=123
PRIMER_PAIR_PENALTY_2=3.2873
PRIMER_LEFT_2_PENALTY=0.074466
PRIMER_RIGHT_2_PENALTY=3.212790
PRIMER_LEFT_2_SEQUENCE=CAGATTCGAGTTTCCAGAATCC
PRIMER_RIGHT_2_SEQUENCE=GGTGTTGAGAATCTGTCTTCATCT
PRIMER_LEFT_2=123,22
PRIMER_RIGHT_2=238,24
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=58.787
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=3.2873
PRIMER_LEFT_3_PENALTY=0.074466
PRIMER_RIGHT_3_PENALTY=3.212790
PRIMER_LEFT_3_SEQUENCE=CAGATTCGAGTTTCCAGAATCC
PRIMER_RIGHT_3_SEQUENCE=AGGTGTTGAGAATCTGTCTTCATC
PRIMER_LEFT_3=123,22
PRIMER_RIGHT_3=239,24
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=58.787
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=117
PRIMER_PAIR_PENALTY_4=3.3014
PRIMER_LEFT_4_PENALTY=0.074466
PRIMER_RIGHT_4_PENALTY=3.226936
PRIMER_LEFT_4_SEQUENCE=CAGATTCGAGTTTCCAGAATCC
PRIMER_RIGHT_4_SEQUENCE=GGTGTTGAGAATCTGTCTTCATC
PRIMER_LEFT_4=123,22
PRIMER_RIGHT_4=238,23
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=57.773
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=116
=
PRIMER_SEQUENCE_ID=SSR_01213 Ps_contig_mira-and-tgicl-ass_franssen_11146
SEQUENCE=GGACAAGAGAAGGATAGAAGGTTGCAGAACCGAATCAAGCAAGAACCCGGAGCGGCATCACAAGGAGGAGAAGAAGATTAAAGCTGATTTGGAAGTTGACGCGCCGAAATCTATCGAGGTATGCACTCAAACGAACTGTTTTCTTTGGATGTGATTTGATGATTTAGATTTCCGATTCGCATTCGCCGTGGCTTGCCGAAGCTTGGAGATTCGACCGATCGTGGTCGTCTTCGATTCACTTGCATTCACCTTCAAGTCGCATGTGTGGTTAAGTTGTGTGTGTGTGGTGTTCAATGTGATACCGG
TARGET=275,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01214 Ps_contig_mira-and-tgicl-ass_franssen_11147
SEQUENCE=AGAAATCAAAGTATSCHGGTCCTCACCTTCATCCTCATTGTCATTCAACACCACACGTACGCCGGCGGAGAACAGACGCGACGATCTCACCGGCATCTCTCTTCCTCGACTCCGGTATCACATTGAACACCACACACACACAACTTAACCACACATGCGACTTGGAAGGTGAATGACAAGTGAATACGAAGACCGACCAGATCGGTAC
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01215 Ps_contig_mira-and-tgicl-ass_franssen_11162
SEQUENCE=AAAACTACTTCACAGACACAAAACAACATCACAGTAAGCAGTTTCTGAGCTGAAACTCAGTGACACTAACCATTTGAATTAAAAAGGAAAAAGATAATTGGTGATTGCAGACATCAGATTCAGCCATGGCCTTTTTTCACCATCATTCTGCAGAGGGAGTATTTTTCATCCTGCATCCGATCTGCTTTATTTTTCATCAGAGTTCTTCTTCTGCATCCGATTTGCTTCATTCTGCATCAGAGTTCTTCTTCTTCTGCATCCGTATTTTCCTTCATTCTGCATCAGTAGTTT
TARGET=243,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9655
PRIMER_LEFT_PENALTY=0.525918
PRIMER_RIGHT_PENALTY=0.439553
PRIMER_LEFT_SEQUENCE=TGGTGATTGCAGACATCAGATT
PRIMER_RIGHT_SEQUENCE=GCAGAATGAAGGAAAATACGGA
PRIMER_LEFT=98,22
PRIMER_RIGHT=279,22
PRIMER_LEFT_TM=60.526
PRIMER_RIGHT_TM=60.440
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=1.2133
PRIMER_LEFT_1_PENALTY=1.027078
PRIMER_RIGHT_1_PENALTY=0.186184
PRIMER_LEFT_1_SEQUENCE=TCTGCAGAGGGAGTATTTTTCA
PRIMER_RIGHT_1_SEQUENCE=GAATGAAGGAAAATACGGATGC
PRIMER_LEFT_1=146,22
PRIMER_RIGHT_1=276,22
PRIMER_LEFT_1_TM=58.973
PRIMER_RIGHT_1_TM=59.814
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=1.4278
PRIMER_LEFT_2_PENALTY=1.241569
PRIMER_RIGHT_2_PENALTY=0.186184
PRIMER_LEFT_2_SEQUENCE=GCAGACATCAGATTCAGCCAT
PRIMER_RIGHT_2_SEQUENCE=GAATGAAGGAAAATACGGATGC
PRIMER_LEFT_2=106,21
PRIMER_RIGHT_2=276,22
PRIMER_LEFT_2_TM=60.242
PRIMER_RIGHT_2_TM=59.814
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=1.4666
PRIMER_LEFT_3_PENALTY=1.027078
PRIMER_RIGHT_3_PENALTY=0.439553
PRIMER_LEFT_3_SEQUENCE=TCTGCAGAGGGAGTATTTTTCA
PRIMER_RIGHT_3_SEQUENCE=GCAGAATGAAGGAAAATACGGA
PRIMER_LEFT_3=146,22
PRIMER_RIGHT_3=279,22
PRIMER_LEFT_3_TM=58.973
PRIMER_RIGHT_3_TM=60.440
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=134
PRIMER_PAIR_PENALTY_4=1.5578
PRIMER_LEFT_4_PENALTY=1.371663
PRIMER_RIGHT_4_PENALTY=0.186184
PRIMER_LEFT_4_SEQUENCE=CATCATTCTGCAGAGGGAGTATT
PRIMER_RIGHT_4_SEQUENCE=GAATGAAGGAAAATACGGATGC
PRIMER_LEFT_4=140,23
PRIMER_RIGHT_4=276,22
PRIMER_LEFT_4_TM=59.628
PRIMER_RIGHT_4_TM=59.814
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_01216 Ps_contig_mira-and-tgicl-ass_franssen_11163
SEQUENCE=TCGGATGCAGGAGAAGAAGAACTCTGATGCAGAATGAAGGAAATCGGATGCAGAAGAAGAAGAACTCTGATGCAGAATGAAGCAAATCGGATGCAGAAGAAGAA
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01217 Ps_contig_mira-and-tgicl-ass_franssen_11201
SEQUENCE=TACCAATCGNTTCCTCNTAATCCACACTACGCGGCTCTCTTGAAGAATTTCACGTGACACATTGAGCGGAAGGTCATTTGAGTCGACCACACCTTTGACAAAACTCAAGTATCTTGGGAACAGTTCTCCATCAAAGTCATCTGAAATAAATACTCTCTTCACATAAAGTCTTATATTCTTGGTCTTGGGATTGATTACGTCATCCTTCCCTGATGGAGCATAGGGAGGAACATAAAGTATAGACCTAAATTCTACCTCGCCCTCTGTGGTAAAGTGTGATGATGCCAACGGCTCCAGGTATTCATTAAATGTTTTCTTGTAAAACTCGTTGTAGTCCTCTTTGGTGACTTCTTTAGGATTACGAAGCCAGATAGGTTGTGTCTCATTTGTGAGCTCCCAGTCCCAATACTTCTCAACAACAGTCTTAGTTTTCTTCTTCTTCTCAGTCTTCTCATCTTGGTTGTCTGTTTTCGCCTCAGCTGGATCCTCATCAACCTCTATTTCTTTGGTGAATCCCTTTTCCTGCCAAGTGTATATTGGGAATGAGACAAATTGGGAGTAGTTTTTCACAAGCTTCTCAATTCGCTCTGGATGAGCAAAACCTTTGTCATCCCTCTTAAGATGCAGGGTAAGGCGAGTTCCTCTTGGAATCAGCTTCTCCCGGTCTGTCTCCTCAGATATGGTATATGAGCTAGCATTTACCTCTCCTTCCCAAATATATTGTTT
TARGET=431,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1553
PRIMER_LEFT_PENALTY=0.008805
PRIMER_RIGHT_PENALTY=0.146525
PRIMER_LEFT_SEQUENCE=AGACCTAAATTCTACCTCGCCC
PRIMER_RIGHT_SEQUENCE=CGAAAACAGACAACCAAGATGA
PRIMER_LEFT=240,22
PRIMER_RIGHT=472,22
PRIMER_LEFT_TM=59.991
PRIMER_RIGHT_TM=60.147
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=12.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.1553
PRIMER_LEFT_1_PENALTY=0.008805
PRIMER_RIGHT_1_PENALTY=0.146525
PRIMER_LEFT_1_SEQUENCE=GACCTAAATTCTACCTCGCCCT
PRIMER_RIGHT_1_SEQUENCE=CGAAAACAGACAACCAAGATGA
PRIMER_LEFT_1=241,22
PRIMER_RIGHT_1=472,22
PRIMER_LEFT_1_TM=59.991
PRIMER_RIGHT_1_TM=60.147
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.1553
PRIMER_LEFT_2_PENALTY=0.008805
PRIMER_RIGHT_2_PENALTY=0.146525
PRIMER_LEFT_2_SEQUENCE=ACCTAAATTCTACCTCGCCCTC
PRIMER_RIGHT_2_SEQUENCE=CGAAAACAGACAACCAAGATGA
PRIMER_LEFT_2=242,22
PRIMER_RIGHT_2=472,22
PRIMER_LEFT_2_TM=59.991
PRIMER_RIGHT_2_TM=60.147
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.2547
PRIMER_LEFT_3_PENALTY=0.108177
PRIMER_RIGHT_3_PENALTY=0.146525
PRIMER_LEFT_3_SEQUENCE=CCTAAATTCTACCTCGCCCTCT
PRIMER_RIGHT_3_SEQUENCE=CGAAAACAGACAACCAAGATGA
PRIMER_LEFT_3=243,22
PRIMER_RIGHT_3=472,22
PRIMER_LEFT_3_TM=60.108
PRIMER_RIGHT_3_TM=60.147
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=0.2826
PRIMER_LEFT_4_PENALTY=0.136083
PRIMER_RIGHT_4_PENALTY=0.146525
PRIMER_LEFT_4_SEQUENCE=ACGGCTCCAGGTATTCATTAAA
PRIMER_RIGHT_4_SEQUENCE=CGAAAACAGACAACCAAGATGA
PRIMER_LEFT_4=287,22
PRIMER_RIGHT_4=472,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=60.147
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_01218 Ps_contig_mira-and-tgicl-ass_franssen_11219
SEQUENCE=TTAACCTAGATTTAGTAGCTATTAGGTCGAAACTTAGAAATTTGGAACCTTTTTTTCTTCTTTTTCGACAAGACTATGACTATGGATGATGTGGATAGTAGCTCTGAGTGGAGCAGGGAGCAAGATAAAGCATTTGAAAATGCTTTGGCTACTTATCCCGAGGACGCTTCAGATCGATGGGAAAAGATTGCGGCGGATGTACCGGGGAAGACTATGGAAGAAATTACACAGCATTATGAGCTCTTGGTTGACGATGTTGGCCAAATTGAAGCTGGTTGTGTTCCAATACCGTCGTATAATTCTTCTTCTGAGGGATCAACGAGTCATGCTAGCGATGAAGGAGTTGGAGGGAAGAAGGGTGGCCATAGTGGTCACAATAACAACGAGTCTAACCATGGAACTAAGGCTTCAAGATCGGATCAAGAACGGCGAAAGGGTATTGCTTGGACTGAGGAAGAACACAGGTTATTTCTTCTGGGCCTAGACAAGTACGGAAAAGGCGACTGGCGAAGTATATCAAGAAACTTTGTGGTAACCCGAACGCCAACACAAGTAGCAAGCCACGCCCAAAAATACTTCATCCGATTGAACTCAATGAACAAAGACAGAAGGCGATCGAGCATACATGATATCACAAGTGTCAACAACGGAGACATCTCAACGCCTCAAGGACCAATCACCGGTCAAACAAACGGTTCCTCCGGAAATTCCGCAAACAAATCAGTCAAACAAGCCGTTCCGTCCACAGCCGGTATACCAGGTGCTGGAATATACGCTGCCCCTTCCATCGGACAACCTATAGGAGGACCTTTAGTATCTGCCGTCGGCACCCCGGTGAACCTTTCCGGATCTCCACATATGGCATACGGTATTCGAGCACCGGTTCCCGGTACCGTTGTTCCCGGTGCACCTATGAACATGGTCCCTATGACATACCCTATGCCTCATACTTCTGGTCCTCACAGGTGAAGTGCTTTTAGAAACCTTAATGTACAAAAAAGTTAGAAGACTAGATTGGCTTCTGCAGTTGATGTAATTTTTTCTTTTTGCTAGACAAAATTGCTTTTTGATTTATTAGCATGCATGCATGAATTTTATTCTTATTCAGTTATTTCAAAAATAAACCATAGCAGGAAGAAATTGAATGTATGTTTCAGTATAGGTTGCCATGAAATGTGACCACTTTGTGACATAGGAGTAAGGTTGTTTTGTAGACATTTGAATTGCTATTTTGCTAAACATTGTTTG
TARGET=1078,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2811
PRIMER_LEFT_PENALTY=0.038361
PRIMER_RIGHT_PENALTY=0.242742
PRIMER_LEFT_SEQUENCE=CATGGTCCCTATGACATACCCT
PRIMER_RIGHT_SEQUENCE=ACAAAGTGGTCACATTTCATGG
PRIMER_LEFT=917,22
PRIMER_RIGHT=1187,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=59.757
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.3818
PRIMER_LEFT_1_PENALTY=0.139083
PRIMER_RIGHT_1_PENALTY=0.242742
PRIMER_LEFT_1_SEQUENCE=GCCTCATACTTCTGGTCCTCAC
PRIMER_RIGHT_1_SEQUENCE=ACAAAGTGGTCACATTTCATGG
PRIMER_LEFT_1=941,22
PRIMER_RIGHT_1=1187,22
PRIMER_LEFT_1_TM=60.139
PRIMER_RIGHT_1_TM=59.757
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.4100
PRIMER_LEFT_2_PENALTY=0.167242
PRIMER_RIGHT_2_PENALTY=0.242742
PRIMER_LEFT_2_SEQUENCE=ACATGGTCCCTATGACATACCC
PRIMER_RIGHT_2_SEQUENCE=ACAAAGTGGTCACATTTCATGG
PRIMER_LEFT_2=916,22
PRIMER_RIGHT_2=1187,22
PRIMER_LEFT_2_TM=59.833
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.4681
PRIMER_LEFT_3_PENALTY=0.225403
PRIMER_RIGHT_3_PENALTY=0.242742
PRIMER_LEFT_3_SEQUENCE=ATTGGCTTCTGCAGTTGATGTA
PRIMER_RIGHT_3_SEQUENCE=ACAAAGTGGTCACATTTCATGG
PRIMER_LEFT_3=1013,22
PRIMER_RIGHT_3=1187,22
PRIMER_LEFT_3_TM=59.775
PRIMER_RIGHT_3_TM=59.757
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.4681
PRIMER_LEFT_4_PENALTY=0.225403
PRIMER_RIGHT_4_PENALTY=0.242742
PRIMER_LEFT_4_SEQUENCE=TGGCTTCTGCAGTTGATGTAAT
PRIMER_RIGHT_4_SEQUENCE=ACAAAGTGGTCACATTTCATGG
PRIMER_LEFT_4=1015,22
PRIMER_RIGHT_4=1187,22
PRIMER_LEFT_4_TM=59.775
PRIMER_RIGHT_4_TM=59.757
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_01219 Ps_contig_mira-and-tgicl-ass_franssen_11224
SEQUENCE=ATACCACCAGCAGCAGCACCAGAAGAAGAAGAAGAAGGATGTTTCTTACATGGAAGAAAATCAGTTAAGTAGAGTAGTAACAATCCATGTGATTGGTATAA
TARGET=21,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01220 Ps_contig_mira-and-tgicl-ass_franssen_11225
SEQUENCE=TAGTTGAAGCTTATACCAATCACACGGATTGTTACTACTCTACTTCTGATTTTCTTCCATGTAAGAAACATCCTTCTTCTTCTTCTGGTGCTGCTGCTGGTGGTATTTGTGCTTACTGTCTTAAGGAGCGTTTGGTGAAGCTTGTGTGTTCAGATTGTGGGGAACAAAGGCTTTCTTCTTGTTCTTGTTCTGATGAGATCACTTCAAATAGAAACTCATGTTCTGTTGAGGTGGGAAGTGTTGGCAGGGTTTCTTTTCTTATTGAGAATGAGAAGAATCAGACAAACCTAATACAGCATTTGAGTTCCAAGGCCAAGATGAATGAGAAAGAAGAAGAGGTTGTGGTGCTGAGAAGAAGCAGCAGCAGCTGTGTTGATATAAAAGGCATGGTTTTGGAAG
TARGET=73,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4072
PRIMER_LEFT_PENALTY=1.346747
PRIMER_RIGHT_PENALTY=0.060408
PRIMER_LEFT_SEQUENCE=AAGCTTATACCAATCACACGGA
PRIMER_RIGHT_SEQUENCE=GTAAGCACAAATACCACCAGCA
PRIMER_LEFT=6,22
PRIMER_RIGHT=115,22
PRIMER_LEFT_TM=58.653
PRIMER_RIGHT_TM=60.060
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=1.4072
PRIMER_LEFT_1_PENALTY=1.346747
PRIMER_RIGHT_1_PENALTY=0.060408
PRIMER_LEFT_1_SEQUENCE=GAAGCTTATACCAATCACACGG
PRIMER_RIGHT_1_SEQUENCE=GTAAGCACAAATACCACCAGCA
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=115,22
PRIMER_LEFT_1_TM=58.653
PRIMER_RIGHT_1_TM=60.060
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=1.4375
PRIMER_LEFT_2_PENALTY=1.377092
PRIMER_RIGHT_2_PENALTY=0.060408
PRIMER_LEFT_2_SEQUENCE=GAAGCTTATACCAATCACACGGA
PRIMER_RIGHT_2_SEQUENCE=GTAAGCACAAATACCACCAGCA
PRIMER_LEFT_2=5,23
PRIMER_RIGHT_2=115,22
PRIMER_LEFT_2_TM=60.377
PRIMER_RIGHT_2_TM=60.060
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=1.4462
PRIMER_LEFT_3_PENALTY=1.346747
PRIMER_RIGHT_3_PENALTY=0.099438
PRIMER_LEFT_3_SEQUENCE=AAGCTTATACCAATCACACGGA
PRIMER_RIGHT_3_SEQUENCE=ACTTCCCACCTCAACAGAACAT
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=58.653
PRIMER_RIGHT_3_TM=59.901
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=1.4462
PRIMER_LEFT_4_PENALTY=1.346747
PRIMER_RIGHT_4_PENALTY=0.099438
PRIMER_LEFT_4_SEQUENCE=GAAGCTTATACCAATCACACGG
PRIMER_RIGHT_4_SEQUENCE=ACTTCCCACCTCAACAGAACAT
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=238,22
PRIMER_LEFT_4_TM=58.653
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_01221 Ps_contig_mira-and-tgicl-ass_franssen_11225
SEQUENCE=TAGTTGAAGCTTATACCAATCACACGGATTGTTACTACTCTACTTCTGATTTTCTTCCATGTAAGAAACATCCTTCTTCTTCTTCTGGTGCTGCTGCTGGTGGTATTTGTGCTTACTGTCTTAAGGAGCGTTTGGTGAAGCTTGTGTGTTCAGATTGTGGGGAACAAAGGCTTTCTTCTTGTTCTTGTTCTGATGAGATCACTTCAAATAGAAACTCATGTTCTGTTGAGGTGGGAAGTGTTGGCAGGGTTTCTTTTCTTATTGAGAATGAGAAGAATCAGACAAACCTAATACAGCATTTGAGTTCCAAGGCCAAGATGAATGAGAAAGAAGAAGAGGTTGTGGTGCTGAGAAGAAGCAGCAGCAGCTGTGTTGATATAAAAGGCATGGTTTTGGAAG
TARGET=357,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01222 Ps_contig_mira-and-tgicl-ass_franssen_11248
SEQUENCE=TCTGGTATTTTTGTTTATTGATTTACATTAATTGATTTCTGGCTGCTTTTTWWTCTAACTGCTTTGAAATTCTCTTGATGGATTGGTTTTTGGTTGCAACTGGCCCTGTGGTTTTGACATTACTTTGTATGAATGATGATGATGAATCTATAGTTTGCCAWATTTAGGTAGT
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01223 Ps_contig_mira-and-tgicl-ass_franssen_11253
SEQUENCE=CATCCGAAACCACATAATCCTCATCGGAATCATCAGAACCACCCCCCTTTGACCTAGCCCTTTTCCTGAAACTACCTTTGCTACTAACCTTCCCTCCCTTTACCATCTGAGAGAGAGAGGAAGAACAAAGCGAAGCACAAAGGATCGAAATTCAAAGAAAAAGTTTCTTTGGG
TARGET=109,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4097
PRIMER_LEFT_PENALTY=0.327913
PRIMER_RIGHT_PENALTY=0.081801
PRIMER_LEFT_SEQUENCE=ACCTAGCCCTTTTCCTGAAACT
PRIMER_RIGHT_SEQUENCE=GAATTTCGATCCTTTGTGCTTC
PRIMER_LEFT=51,22
PRIMER_RIGHT=152,22
PRIMER_LEFT_TM=59.672
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=0.5225
PRIMER_LEFT_1_PENALTY=0.440748
PRIMER_RIGHT_1_PENALTY=0.081801
PRIMER_LEFT_1_SEQUENCE=ACCACATAATCCTCATCGGAAT
PRIMER_RIGHT_1_SEQUENCE=GAATTTCGATCCTTTGTGCTTC
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=152,22
PRIMER_LEFT_1_TM=59.559
PRIMER_RIGHT_1_TM=60.082
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=0.6128
PRIMER_LEFT_2_PENALTY=0.530964
PRIMER_RIGHT_2_PENALTY=0.081801
PRIMER_LEFT_2_SEQUENCE=CCACATAATCCTCATCGGAATC
PRIMER_RIGHT_2_SEQUENCE=GAATTTCGATCCTTTGTGCTTC
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=152,22
PRIMER_LEFT_2_TM=60.531
PRIMER_RIGHT_2_TM=60.082
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=0.6439
PRIMER_LEFT_3_PENALTY=0.562082
PRIMER_RIGHT_3_PENALTY=0.081801
PRIMER_LEFT_3_SEQUENCE=CCGAAACCACATAATCCTCATC
PRIMER_RIGHT_3_SEQUENCE=GAATTTCGATCCTTTGTGCTTC
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=152,22
PRIMER_LEFT_3_TM=60.562
PRIMER_RIGHT_3_TM=60.082
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=150
PRIMER_PAIR_PENALTY_4=0.6439
PRIMER_LEFT_4_PENALTY=0.562082
PRIMER_RIGHT_4_PENALTY=0.081801
PRIMER_LEFT_4_SEQUENCE=GAAACCACATAATCCTCATCGG
PRIMER_RIGHT_4_SEQUENCE=GAATTTCGATCCTTTGTGCTTC
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=152,22
PRIMER_LEFT_4_TM=60.562
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.8000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_01224 Ps_contig_mira-and-tgicl-ass_franssen_11254
SEQUENCE=AAAGCGAAGCACAAAGGATCGAAATTCAAGAAAAAGTTTCTTTGGGGCAGTTTCTTTAACGGTTTGGGTTTAATAATAAATTAATATTTAATTATGCATTTTTCTTTTTTCTTTTCAAAATTTTAGAGAGAGAGAGAGAGATTCAGATTTTTCATTCCCCCCGCGTACT
TARGET=125,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.5464
PRIMER_LEFT_PENALTY=0.354011
PRIMER_RIGHT_PENALTY=3.192432
PRIMER_LEFT_SEQUENCE=TCTTTGGGGCAGTTTCTTTAAC
PRIMER_RIGHT_SEQUENCE=GGGGGAATGAAAAATCTGAA
PRIMER_LEFT=38,22
PRIMER_RIGHT=160,20
PRIMER_LEFT_TM=59.646
PRIMER_RIGHT_TM=58.808
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=3.6876
PRIMER_LEFT_1_PENALTY=0.495211
PRIMER_RIGHT_1_PENALTY=3.192432
PRIMER_LEFT_1_SEQUENCE=AGTTTCTTTGGGGCAGTTTCTT
PRIMER_RIGHT_1_SEQUENCE=GGGGGAATGAAAAATCTGAA
PRIMER_LEFT_1=34,22
PRIMER_RIGHT_1=160,20
PRIMER_LEFT_1_TM=60.495
PRIMER_RIGHT_1_TM=58.808
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=3.6876
PRIMER_LEFT_2_PENALTY=0.495211
PRIMER_RIGHT_2_PENALTY=3.192432
PRIMER_LEFT_2_SEQUENCE=AAGTTTCTTTGGGGCAGTTTCT
PRIMER_RIGHT_2_SEQUENCE=GGGGGAATGAAAAATCTGAA
PRIMER_LEFT_2=33,22
PRIMER_RIGHT_2=160,20
PRIMER_LEFT_2_TM=60.495
PRIMER_RIGHT_2_TM=58.808
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=128
PRIMER_PAIR_PENALTY_3=3.7428
PRIMER_LEFT_3_PENALTY=0.550413
PRIMER_RIGHT_3_PENALTY=3.192432
PRIMER_LEFT_3_SEQUENCE=CAGTTTCTTTAACGGTTTGGGT
PRIMER_RIGHT_3_SEQUENCE=GGGGGAATGAAAAATCTGAA
PRIMER_LEFT_3=47,22
PRIMER_RIGHT_3=160,20
PRIMER_LEFT_3_TM=59.450
PRIMER_RIGHT_3_TM=58.808
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=4.0172
PRIMER_LEFT_4_PENALTY=0.824786
PRIMER_RIGHT_4_PENALTY=3.192432
PRIMER_LEFT_4_SEQUENCE=GTTTCTTTGGGGCAGTTTCTTT
PRIMER_RIGHT_4_SEQUENCE=GGGGGAATGAAAAATCTGAA
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=160,20
PRIMER_LEFT_4_TM=60.825
PRIMER_RIGHT_4_TM=58.808
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_01225 Ps_contig_mira-and-tgicl-ass_franssen_11260
SEQUENCE=GAAACAAACATTTCACSAACAACTTTAGGTTACAATAGAGATTCTTCTTCTTCTTCACCAACTCCACCTCAAACAACTCCATCTCAAACTAACACAAACACATTAGTCTTCAATATTCCACCACAAAATCCACAACACAACAATAATCTTCATCCAGATCCAGATCTAGTCGTTAC
TARGET=42,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01226 Ps_contig_mira-and-tgicl-ass_franssen_11261
SEQUENCE=TTAGTTTGAGATGGAGTGTTTNAGGTGGAGTTGGTGAGAAGAAGAAGAATCTCTATTGTAACCTAAAGTTGTTGGTTGAAATGTTTGTTTCCATTAACCTTATTGTGAGGAGGGTGTGTGTGTGTGAGAGAGATCTAAATTAGAATAATCTCTCTCTAACTTAAACCATAATCAATGATTATTTTGTGTTTAACTATGGTTATGGATGCTAAACCCTAATTCAACACTATCTAATAYACAAGAACAAAACTTAAACAATGATGATTATAGTAATAGTAGTAGTAGAAGAGAGATACTGTTATTTATTGTGTG
TARGET=37,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01227 Ps_contig_mira-and-tgicl-ass_franssen_11261
SEQUENCE=TTAGTTTGAGATGGAGTGTTTNAGGTGGAGTTGGTGAGAAGAAGAAGAATCTCTATTGTAACCTAAAGTTGTTGGTTGAAATGTTTGTTTCCATTAACCTTATTGTGAGGAGGGTGTGTGTGTGTGAGAGAGATCTAAATTAGAATAATCTCTCTCTAACTTAAACCATAATCAATGATTATTTTGTGTTTAACTATGGTTATGGATGCTAAACCCTAATTCAACACTATCTAATAYACAAGAACAAAACTTAAACAATGATGATTATAGTAATAGTAGTAGTAGAAGAGAGATACTGTTATTTATTGTGTG
TARGET=114,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01228 Ps_contig_mira-and-tgicl-ass_franssen_11261
SEQUENCE=TTAGTTTGAGATGGAGTGTTTNAGGTGGAGTTGGTGAGAAGAAGAAGAATCTCTATTGTAACCTAAAGTTGTTGGTTGAAATGTTTGTTTCCATTAACCTTATTGTGAGGAGGGTGTGTGTGTGTGAGAGAGATCTAAATTAGAATAATCTCTCTCTAACTTAAACCATAATCAATGATTATTTTGTGTTTAACTATGGTTATGGATGCTAAACCCTAATTCAACACTATCTAATAYACAAGAACAAAACTTAAACAATGATGATTATAGTAATAGTAGTAGTAGAAGAGAGATACTGTTATTTATTGTGTG
TARGET=274,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01229 Ps_contig_mira-and-tgicl-ass_franssen_11288
SEQUENCE=CCTTTACCTTACCCTAACCTAACTAGTTCWAGAAAAGAACCTTTGAATCTCTTTCTTTGTCTTTGTAAAACAACTTTTGATCTTGATGATGTAATCCAGTTGGTCCTGTCAACATTTTCTTCAAATCCCCAAATGTGGCTTGTGAGCTAATGTTGAGTTCATGATAAATTGAACCAAACTTGACCCTAACTCTAATAGTTGGAACCAAAACCGGGTTTTGATCTGAGTTATTGGTTCTAGTTTGAACCAACATTCCACCTGGCCTCATCTCCCAATCCATTGAACCGGGTTCAGTTCTGCAACCACCTGAGGTTATAGGTGAAGAAACAMCCATTTGTCTTATTACTATGATTATGATTATTCCTCATCCTCATCATCTTCTTTCACTTTCACAGCTATGCAACAAAAGAGGTATGAACTGAGAACTATGAGAGAGAGAGAGAGAGAGAGGGGGGGTTAGATCTCTGAGAACACAGATTCTCTAGAAAACTTGACAGGAAATTCACCGACCCAATTCACCAAAAGGCTGCTAGAACATGTTACAGAAACCTCTGACCAAAGCATTCCACAAGTCCACCATCACAAGAGAGAGAAAAAAACAAAAGCTTTGAACTTCAAGAGAGAGAGAAATAAAAACAAAAAATTGAAACTTTTTCT
TARGET=430,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01230 Ps_contig_mira-and-tgicl-ass_franssen_11288
SEQUENCE=CCTTTACCTTACCCTAACCTAACTAGTTCWAGAAAAGAACCTTTGAATCTCTTTCTTTGTCTTTGTAAAACAACTTTTGATCTTGATGATGTAATCCAGTTGGTCCTGTCAACATTTTCTTCAAATCCCCAAATGTGGCTTGTGAGCTAATGTTGAGTTCATGATAAATTGAACCAAACTTGACCCTAACTCTAATAGTTGGAACCAAAACCGGGTTTTGATCTGAGTTATTGGTTCTAGTTTGAACCAACATTCCACCTGGCCTCATCTCCCAATCCATTGAACCGGGTTCAGTTCTGCAACCACCTGAGGTTATAGGTGAAGAAACAMCCATTTGTCTTATTACTATGATTATGATTATTCCTCATCCTCATCATCTTCTTTCACTTTCACAGCTATGCAACAAAAGAGGTATGAACTGAGAACTATGAGAGAGAGAGAGAGAGAGAGGGGGGGTTAGATCTCTGAGAACACAGATTCTCTAGAAAACTTGACAGGAAATTCACCGACCCAATTCACCAAAAGGCTGCTAGAACATGTTACAGAAACCTCTGACCAAAGCATTCCACAAGTCCACCATCACAAGAGAGAGAAAAAAACAAAAGCTTTGAACTTCAAGAGAGAGAGAAATAAAAACAAAAAATTGAAACTTTTTCT
TARGET=618,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01231 Ps_contig_mira-and-tgicl-ass_franssen_11296
SEQUENCE=CAGATTCGCAACCTTGCTTACGACCCGAGATCGCTTTGCTTTTGACGCTTCTAGCTGCGAGCTCGAGATGATGATGATTGTTGCAGACCAAAYSATGAKTACTACTATTCAACACCTCATAACCTACCCGAAACCAAAACCATCCTACCAACAATTCACCCACCCATCCTCACAATCACCACTCCCAACTACCCAAAAATCAACGCCTTAGA
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01232 Ps_contig_mira-and-tgicl-ass_franssen_11314
SEQUENCE=TCGGGGGTATGATTCACGTTCAACACCTTGTGAACCCTTGAAACTTTTGTTGCCAGAAGCTTCCACCTCCACCGTGTCGGAAATTACCTTTGGTGGCGGAGGACCAAACACCAAAAACCATATATCAAAACCTTCGTTTCGATGTCCTTAACTCAATTCCAACATCAGTTTCACGAGATTCACAATCAAATGCCCTAACTGAAACGCTTTGTTCAAGAACCCTAAACACGAAAATCAAAATTAAATCCTAATTACATGGAATCAAAGCCTGAATTTTTGGGGCTTTGATTCCTCACACCCCAAGCTACATAACAGTAATCGATTCGAAGCAAAATGGTGAGCAAAGTGAACCTAACTGCCGGAGAAGACGAAGATGAAGACGATTTCGATAAGTTCTTGTTCTGACTCCTTTCTTCCTTATGAATTCAAATGATTCTTCTTCTCCTTCTTCTTCTACTACTCAGTTGAATTTGCTACGAGGTTGTTGAATTGAGAGGGTGAAGGATGGAATTGAGAGAGTTATGTGAGAGTGGTTCTGAAAGGGTTCATGTGAATTTTTAGAAAGATGTAGGAATCAAAGAGTGTGTGTGTGAATGAGTTTCTGAAAAATGAATGAATCCCCCAGATCAATGAATGTCTAGGGTGAATGATTTATAGGGTTTAGAGTTAACAAAGATCAACTCTGTTAGTTTGAATCAAAGCCAAAACGGTTTGAAGTTAATTACTCTGTTACTTTAGGGATTCATGAAAGTTTTTTAGTTGGAAATTATGTTCGGAAGTTAGTTAAGCCCGTGTAAGTTAGCTTCTGTTATATTTTTGATTAAGTTAAAATAAATTCTGTTAAAATGAAGTTATGTTATGAAACTCTATTAGTACTATTAGCAGATATGTGCATATTAACTGAAAAATCATGTTAACATATGTCAGTAAGTGTTCTGTGGTTTGGGCTTATGGA
TARGET=582,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1774
PRIMER_LEFT_PENALTY=0.145769
PRIMER_RIGHT_PENALTY=0.031678
PRIMER_LEFT_SEQUENCE=TGAGAGTGGTTCTGAAAGGGTT
PRIMER_RIGHT_SEQUENCE=CAGAAGCTAACTTACACGGGCT
PRIMER_LEFT=524,22
PRIMER_RIGHT=807,22
PRIMER_LEFT_TM=60.146
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.2996
PRIMER_LEFT_1_PENALTY=0.267873
PRIMER_RIGHT_1_PENALTY=0.031678
PRIMER_LEFT_1_SEQUENCE=GTGGTTCTGAAAGGGTTCATGT
PRIMER_RIGHT_1_SEQUENCE=CAGAAGCTAACTTACACGGGCT
PRIMER_LEFT_1=529,22
PRIMER_RIGHT_1=807,22
PRIMER_LEFT_1_TM=60.268
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.2996
PRIMER_LEFT_2_PENALTY=0.145769
PRIMER_RIGHT_2_PENALTY=0.153836
PRIMER_LEFT_2_SEQUENCE=TGAGAGTGGTTCTGAAAGGGTT
PRIMER_RIGHT_2_SEQUENCE=ACAGAAGCTAACTTACACGGGC
PRIMER_LEFT_2=524,22
PRIMER_RIGHT_2=808,22
PRIMER_LEFT_2_TM=60.146
PRIMER_RIGHT_2_TM=59.846
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.3866
PRIMER_LEFT_3_PENALTY=0.354881
PRIMER_RIGHT_3_PENALTY=0.031678
PRIMER_LEFT_3_SEQUENCE=GTGAGAGTGGTTCTGAAAGGGT
PRIMER_RIGHT_3_SEQUENCE=CAGAAGCTAACTTACACGGGCT
PRIMER_LEFT_3=523,22
PRIMER_RIGHT_3=807,22
PRIMER_LEFT_3_TM=59.645
PRIMER_RIGHT_3_TM=59.968
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.4217
PRIMER_LEFT_4_PENALTY=0.267873
PRIMER_RIGHT_4_PENALTY=0.153836
PRIMER_LEFT_4_SEQUENCE=GTGGTTCTGAAAGGGTTCATGT
PRIMER_RIGHT_4_SEQUENCE=ACAGAAGCTAACTTACACGGGC
PRIMER_LEFT_4=529,22
PRIMER_RIGHT_4=808,22
PRIMER_LEFT_4_TM=60.268
PRIMER_RIGHT_4_TM=59.846
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=12.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_01233 Ps_contig_mira-and-tgicl-ass_franssen_11323
SEQUENCE=CCCGACGTAACGAAGTTACCTATCTTACTATCCTCCTTGTCAAAGACATCAGGTGGAGGTTTTGGTTTCGTTTCTATGATGATTTTGGTAGAAGCTTTKGKTGTTAATTTACTAAAGGGACATCTGCCAGATCAGCAGGCTCCCATGGACTGTTAGAATTGCGGACATCAGATGATATTCTCATTACACAAGTTGTGGGTCCAGGGGAAGAATCACACGTAGACACCCATGAAACATTTAAAAATGACRCCGCTCTTTATATTCAAATGCTAAGTGTTGAAGAGACACAGAGCTTCTCTCTCTCTATGGAAACCAAAGGAGATATCAAAAGTGATTTTGTCTTTTTCCAGTTTGCTATTCAGTATTCAAATGTGTATCAGGCTGATGTTTCAAGGGTTGTTACTGTTAGATTGCCTACAGTAGACAGTGTTTCGGG
TARGET=295,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01234 Ps_contig_mira-and-tgicl-ass_franssen_11331
SEQUENCE=TAGAAACCGTTCAAGAAAGCCTGTGGAACTAGTTGTGTCTTCATCATCATCAACAACAACACACACAAACTCAATCTCTCTATCTCAATCTCAATCTCAATCTCATTCTCAAACAAATACCAGAAACCTCTCCTTGAACAACAACAACAACAACAACACCTTAACACCACCTTCTTCTTTTCCTTTTCATGAATCTCAACAACAAGCCTTTTCACAACCCTACCAAAACCCTTACTTTTATTCTCAGCAATCTGTTACTGGTTCTAAACCTCTTGATCATTCTGAGTTTCAACCTCATGACGGTTCTACTACTCATCACCTTCTTTTTGGATTCT
TARGET=41,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3275
PRIMER_LEFT_PENALTY=0.205226
PRIMER_RIGHT_PENALTY=0.122257
PRIMER_LEFT_SEQUENCE=GTTCAAGAAAGCCTGTGGAACT
PRIMER_RIGHT_SEQUENCE=GGTTTTGGTAGGGTTGTGAAAA
PRIMER_LEFT=8,22
PRIMER_RIGHT=229,22
PRIMER_LEFT_TM=59.795
PRIMER_RIGHT_TM=60.122
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.3407
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.135429
PRIMER_LEFT_1_SEQUENCE=GTTCAAGAAAGCCTGTGGAACT
PRIMER_RIGHT_1_SEQUENCE=TAAGGGTTTTGGTAGGGTTGTG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=233,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=60.135
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.4472
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.242013
PRIMER_LEFT_2_SEQUENCE=GTTCAAGAAAGCCTGTGGAACT
PRIMER_RIGHT_2_SEQUENCE=TTTTGGTAGGGTTGTGAAAAGG
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.5119
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.306698
PRIMER_LEFT_3_SEQUENCE=GTTCAAGAAAGCCTGTGGAACT
PRIMER_RIGHT_3_SEQUENCE=GTTGTGAAAAGGCTTGTTGTTG
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=217,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=59.693
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=0.5231
PRIMER_LEFT_4_PENALTY=0.205226
PRIMER_RIGHT_4_PENALTY=0.317850
PRIMER_LEFT_4_SEQUENCE=GTTCAAGAAAGCCTGTGGAACT
PRIMER_RIGHT_4_SEQUENCE=TGTGAAAAGGCTTGTTGTTGAG
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=215,22
PRIMER_LEFT_4_TM=59.795
PRIMER_RIGHT_4_TM=60.318
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_01235 Ps_contig_mira-and-tgicl-ass_franssen_11331
SEQUENCE=TAGAAACCGTTCAAGAAAGCCTGTGGAACTAGTTGTGTCTTCATCATCATCAACAACAACACACACAAACTCAATCTCTCTATCTCAATCTCAATCTCAATCTCATTCTCAAACAAATACCAGAAACCTCTCCTTGAACAACAACAACAACAACAACACCTTAACACCACCTTCTTCTTTTCCTTTTCATGAATCTCAACAACAAGCCTTTTCACAACCCTACCAAAACCCTTACTTTTATTCTCAGCAATCTGTTACTGGTTCTAAACCTCTTGATCATTCTGAGTTTCAACCTCATGACGGTTCTACTACTCATCACCTTCTTTTTGGATTCT
TARGET=137,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3275
PRIMER_LEFT_PENALTY=0.205226
PRIMER_RIGHT_PENALTY=0.122257
PRIMER_LEFT_SEQUENCE=GTTCAAGAAAGCCTGTGGAACT
PRIMER_RIGHT_SEQUENCE=GGTTTTGGTAGGGTTGTGAAAA
PRIMER_LEFT=8,22
PRIMER_RIGHT=229,22
PRIMER_LEFT_TM=59.795
PRIMER_RIGHT_TM=60.122
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.3407
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.135429
PRIMER_LEFT_1_SEQUENCE=GTTCAAGAAAGCCTGTGGAACT
PRIMER_RIGHT_1_SEQUENCE=TAAGGGTTTTGGTAGGGTTGTG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=233,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=60.135
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.4472
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.242013
PRIMER_LEFT_2_SEQUENCE=GTTCAAGAAAGCCTGTGGAACT
PRIMER_RIGHT_2_SEQUENCE=TTTTGGTAGGGTTGTGAAAAGG
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.5119
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.306698
PRIMER_LEFT_3_SEQUENCE=GTTCAAGAAAGCCTGTGGAACT
PRIMER_RIGHT_3_SEQUENCE=GTTGTGAAAAGGCTTGTTGTTG
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=217,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=59.693
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=0.5231
PRIMER_LEFT_4_PENALTY=0.205226
PRIMER_RIGHT_4_PENALTY=0.317850
PRIMER_LEFT_4_SEQUENCE=GTTCAAGAAAGCCTGTGGAACT
PRIMER_RIGHT_4_SEQUENCE=TGTGAAAAGGCTTGTTGTTGAG
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=215,22
PRIMER_LEFT_4_TM=59.795
PRIMER_RIGHT_4_TM=60.318
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_01236 Ps_contig_mira-and-tgicl-ass_franssen_11333
SEQUENCE=GCAATGAGCATATAAAATAAATATAGAGAAATARAAAACTATTGGGCTACTCTGCTAATTTGTTTTGTGATGTCAAGCTTCTTGATATAAATGCAGTTCATCAAGAAAGATCCAAGTCATTATATCTTTGTCTTGGGCCTACTGAACTCCTATCCAACGAGCATAATCCACCCATAATAATGAACCCAAATATGTATTGCCAGTTCGAACAGCAAAACAAAGAGCACCAAGTATAAGTTTGTGCTGGTGCAACATAGGTTCCAAAAGCCTTTGTTCAATAACTCCCGCAACAACTTGGTACCTGAGATTGCTAGAAACTGCCATATAAACACCATAAGCAGCACTGGTAGACAATATGGGAACATTTTCAATTTCCTCTGCAGAAGACTTGTTAACAGCCTTCTTTGCATTAATTAACGCGTTCGTCACAGCTGTCCCAACCAGTGATGCACCAGTTCCAACTGCAAAAAGCTTGGACCCATTACGCACAATTGCACCAAATCTCTGCAAGAAGGAATATGATGTTCCAGAGAGAGCAACCTGGAATGCATTATCTGGGCAGTTGTGGAAAAACTTAGAAATAGCCCCTGCACTAACTCCAAGTGGTGGTCTAAGAGAAACAGTAGGAGCAGGAAGATAAACTAGCATGAAATCTGCGAGTATAGCCATGACCACATCGGCGAACACAATTTCAAGCTCGTTGAAAAAGTTCTCTCTCCTACGATCATACTCGGCGGCAGTCTTGGCAATGACGCCAACACCGCACTCCATACCGAGCTTAGCGAGGAAAAGATCATCAGCGAGAAGACGCTCTCTGAAACCGGTAAACTTAAGCAGCCACTGCATGAACGGAGACTTCTCAAGTTCGAGAAACCGAGCAACGACGGCGGCAGGAATCTTACCGTCCTTGATAGCGGAAGCTAGATCAGCAGGCACACTCTCCAACGACCTACCTGCTTGCGCCAACAACAGAAGCGCCTCGTTAACTACGTTGTGTCCGTCATTGTTTTCTCCGTCTCCGTCATTGGCGTCTCCGCCGCCACCGTGTCCGCCGAACGAGCCGCCATCGTCGTGACCTCCGAAACCTCCGCCGGAGTCCGTGGAATGGAGAATGAAGAGAGGATCTGCGCGGCGGTTGAGGCGGAGAGAAGAAGAGGGAGATGGAGTGAGACGGAGATGGAGAGAAGATGAAGAGAAGGAAATGGAAGGGATGGATTTGGGAGTGGAAGGAGAATGGGAGGAAAGAGAATGGGAAGAAGGAGGAACGAAGGAAAAAGCCATCGACATTGTTTCTTCTGTGATTCGGTTTTGGCTTTTCAGTGACTTACGAGGCTGCGCACGACTCAGGGGTTTTGAGTTGAGTTGAGTTGAGTTACGCTGTTATGACTTATSACTTGTC
TARGET=1353,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01237 Ps_contig_mira-and-tgicl-ass_franssen_11370
SEQUENCE=TTATCATAAAATATATATATTTTTGATTGAATATTAAAAATTATAAGTATCAACAAATTGAATCATTTCCATGACACATAAGGCTAAAAAACAAAGCCTTCACAACATTTGGGAAACAGAAATCCTCATTTTCCCACTTTACCAAAACAGAATTTCTTTGCTGCTCCACTCTTTGAAGATGAGAAGACAGTTAGAAACAAATCAACAGTAACTCCATTTCAGATTTGATAACTCCCTTAGAGTTTATCTCAAGTAGAATAAAGATTCATTTCGTCATTGTTTA
TARGET=11,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01238 Ps_contig_mira-and-tgicl-ass_franssen_11373
SEQUENCE=CATCAGGGGCACCCGGCATACCAATCACTTTCAAGCTCCACATCTTTTGGCGGCCTGCATCATCAACCAAAACAATTTTCCTGGGAGTCAGATGTGAACAGACCACAACCTTCTTTGGGCAGCAGGTTGCCTCCTGAAGGACATTTCAGTACTTCCTCCCAGATTCATCCATTAGCACAACAACAACAATCAGTTCATAATTTCAATATACTTC
TARGET=177,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01239 Ps_contig_mira-and-tgicl-ass_franssen_11374
SEQUENCE=ATCCATTAGCACAACAACAACAATCAGTTCATAATTTTCAATATACTTCATCTGATGTTAATTTGACCGGACCTGGAGGAACTGCCACTGTATCCAGATATCCACCAGATATTCCTGATAGCAACCATTCAACTTCTCTTCCGGCTT
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01240 Ps_contig_mira-and-tgicl-ass_franssen_11380
SEQUENCE=TGTCACTGTTCCTTCAGTTGAATATGGCCAAGCTTTTGCTTTAGAGTTCATTATCAGCTTTAATCTCATGTTTGTTGTCACTGCAGTTGCAACCGACACAAGAGCTGTGGGAGAGCTTGCGGGAATCGCGGTGGGAGCCACCGTCATGCTCAACATACTCATTGCTGGGCCTGCAACTGGAGGTTCAATGAATCCAGTAAGAACATTGGGCCCAGCAATTGCTGCAAACAACTACAAAGGCATATGGCTATATATCATAGCTCCTATTCTTGGAGCTTTAGCTGGGGCAGGTGCCTACACTGCTGTTAAACTCCCTGATGAAGATTTTAACTCTGAAGTAAAAGCCTCTTCTGCCCCTGGAAGCTTTAGGAGATGATCTCATCTCATCCACAAGGTTGCAATACAAGATCTAACAAAGCTACTTTAAATAGAACTTTTTTATTATATAAGTACATTATAGTTTGTAATTAAGAATGAGTGAGTGAGTACTGCAATTGATGCATCCTTAGCTTGCTAGCTACATGCGCATGTTGTAATATTTGTGTTCAAATGCATCAATTAGTTGTGCAACGTTATGATCCAAGATGAATTAGTGTTGTTTCTGTACACMTGTATATGCTATTTGTTTTTTCTTTTAW
TARGET=475,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01241 Ps_contig_mira-and-tgicl-ass_franssen_11395
SEQUENCE=TTTAAGCAAAACTACGCGATTTAATCCTATCTTTAATTCCAGAATATCAAAACAGCAAGTGGCTAAAATTATAGAATGTCAACCCATATTCATCCCAGAATCGGAACGGCAATCAGCATAATCAAATGAAAACAAAACATATCCAACATGACCATATCAAAAATACAGCAGAGTAAACCTAAACATTAAACCGAATCAAGAGATGAAATTTAAAAAGGAAAATCCAACCGTTTCTGCGGCGTCGACTCGGGCTTTCTCAGGAACTAATGACTCCGAATCGTATTTGGAGTTCGACTTTGTGCTCAACCGTTCCAGTGCTGATCTGAACCGAAAACTTCGTCGGTATAGTTGCCGGTTCGTGTCGTCAATCTGCGTTTTAACCAAGAACCGAACGGAACCCTTCGATACCACAATCAACCAAAATCCAATTCCAATTGAAACAAGCACATAACCTGCATAAAAAACAGACAAATGAAGCAGCACAAGCTTCACATATTTGGAAGCGGAAAAACAGAGTTTGGTTCGAAAATGTGGATTTTGATACGGTAGCGGCGGAAATAGAGGGTTATTGAGTTTGAGAGGTGTAAGGCTGGGTTYGGCGAAAGAAGGTTTGAGAAGCGTAGTATGGTGTGGTGTCGCGGAGGGTTTCGGTAAAGGGGGTNATGGAAGGCAGGCGACGGGTGGTGGGCGGAAGGGGAGCGGGCGGGTAAGGAAGGAAGGTAGGTTTTGGAGTTGGGTAAGG
TARGET=709,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01242 Ps_contig_mira-and-tgicl-ass_franssen_11412
SEQUENCE=AGCCGGAACTGCTAATTCGCCGGCAAAAATGGAGTCAGACTCGCCGTTACCGGAAAACATTATGGAATTAGAATTTCCGTCGTCTTCTATTCGGTCATGCTCAGAGCCAGAGGTGGTGCTGTTTTTCTCATGTTCTTCTTCTTCCTCCTCGTAATTATAAATTTCTTTTCCGACAGAACCATGTTGAAATTCGCCGTTTGAAAAATCAAGCAAGTCATCGACAGAAAAATCTTCACCGACAACAACGTTATTCGCGTTCAAACACAAAATTTCCTCGCTAATCGTTTGTGAAAAAACAGACTCTGTCCTCAAGCTTGGTTTCAACGCTTCCGCCACCACAATTTCCATTATTCTCTTCTCTGAAT
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3895
PRIMER_LEFT_PENALTY=0.312177
PRIMER_RIGHT_PENALTY=0.077340
PRIMER_LEFT_SEQUENCE=TGGAATTAGAATTTCCGTCGTC
PRIMER_RIGHT_SEQUENCE=AACCAAGCTTGAGGACAGAGTC
PRIMER_LEFT=62,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=60.312
PRIMER_RIGHT_TM=59.923
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.4077
PRIMER_LEFT_1_PENALTY=0.312177
PRIMER_RIGHT_1_PENALTY=0.095538
PRIMER_LEFT_1_SEQUENCE=TGGAATTAGAATTTCCGTCGTC
PRIMER_RIGHT_1_SEQUENCE=GAATAACGTTGTTGTCGGTGAA
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=252,22
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.4303
PRIMER_LEFT_2_PENALTY=0.312177
PRIMER_RIGHT_2_PENALTY=0.118120
PRIMER_LEFT_2_SEQUENCE=TGGAATTAGAATTTCCGTCGTC
PRIMER_RIGHT_2_SEQUENCE=TCTGTCGATGACTTGCTTGATT
PRIMER_LEFT_2=62,22
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=59.882
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=0.4303
PRIMER_LEFT_3_PENALTY=0.312177
PRIMER_RIGHT_3_PENALTY=0.118120
PRIMER_LEFT_3_SEQUENCE=TGGAATTAGAATTTCCGTCGTC
PRIMER_RIGHT_3_SEQUENCE=TTCTGTCGATGACTTGCTTGAT
PRIMER_LEFT_3=62,22
PRIMER_RIGHT_3=225,22
PRIMER_LEFT_3_TM=60.312
PRIMER_RIGHT_3_TM=59.882
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.4472
PRIMER_LEFT_4_PENALTY=0.369874
PRIMER_RIGHT_4_PENALTY=0.077340
PRIMER_LEFT_4_SEQUENCE=AATTTCCGTCGTCTTCTATTCG
PRIMER_RIGHT_4_SEQUENCE=AACCAAGCTTGAGGACAGAGTC
PRIMER_LEFT_4=71,22
PRIMER_RIGHT_4=319,22
PRIMER_LEFT_4_TM=59.630
PRIMER_RIGHT_4_TM=59.923
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.6000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_01243 Ps_contig_mira-and-tgicl-ass_franssen_11435
SEQUENCE=CCAACCCTTGTCGAAATCGAAAAACCCCAGTAACTTAACTCACATAAAGATCCAATTTCGCATTGCTAAACAACACAAAAACCTTCATCAAACAAAAACCCACAGACGGATCCAAATTTTCCCCTTATGTAATTGAAAATTGAAAGAAAAAAATAATAATAATTTGGGAGTTGAATTGTAGTTGGATTGTTCCTTAAAG
TARGET=152,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1326
PRIMER_LEFT_PENALTY=0.603423
PRIMER_RIGHT_PENALTY=0.529160
PRIMER_LEFT_SEQUENCE=CCTTCATCAAACAAAAACCCAC
PRIMER_RIGHT_SEQUENCE=TCCAACTACAATTCAACTCCCA
PRIMER_LEFT=81,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=60.603
PRIMER_RIGHT_TM=59.471
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=105
PRIMER_PAIR_PENALTY_1=1.1510
PRIMER_LEFT_1_PENALTY=0.621889
PRIMER_RIGHT_1_PENALTY=0.529160
PRIMER_LEFT_1_SEQUENCE=CATCAAACAAAAACCCACAGAC
PRIMER_RIGHT_1_SEQUENCE=TCCAACTACAATTCAACTCCCA
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=59.378
PRIMER_RIGHT_1_TM=59.471
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=1.6201
PRIMER_LEFT_2_PENALTY=1.090970
PRIMER_RIGHT_2_PENALTY=0.529160
PRIMER_LEFT_2_SEQUENCE=GAAATCGAAAAACCCCAGTAAC
PRIMER_RIGHT_2_SEQUENCE=TCCAACTACAATTCAACTCCCA
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=185,22
PRIMER_LEFT_2_TM=58.909
PRIMER_RIGHT_2_TM=59.471
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=1.6804
PRIMER_LEFT_3_PENALTY=1.151217
PRIMER_RIGHT_3_PENALTY=0.529160
PRIMER_LEFT_3_SEQUENCE=ATCAAACAAAAACCCACAGACG
PRIMER_RIGHT_3_SEQUENCE=TCCAACTACAATTCAACTCCCA
PRIMER_LEFT_3=86,22
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=61.151
PRIMER_RIGHT_3_TM=59.471
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=2.3454
PRIMER_LEFT_4_PENALTY=1.816208
PRIMER_RIGHT_4_PENALTY=0.529160
PRIMER_LEFT_4_SEQUENCE=CTTGTCGAAATCGAAAAACCC
PRIMER_RIGHT_4_SEQUENCE=TCCAACTACAATTCAACTCCCA
PRIMER_LEFT_4=6,21
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=60.816
PRIMER_RIGHT_4_TM=59.471
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=180
=
PRIMER_SEQUENCE_ID=SSR_01244 Ps_contig_mira-and-tgicl-ass_franssen_11438
SEQUENCE=AGCTTATTCGGCTATAAAGAATATGTGAAGTCGGTATAATGTATTTGTATATGTRTTGTTTAAAAAAACATGTATATGTATATATATATTTTGAGATACAGGCCTGAATCATATGTAATTATTTAGTATCCTTTTCATTTTTTTTAATAAGATTTCTTTTGAGAATCTTCTAACC
TARGET=79,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01245 Ps_contig_mira-and-tgicl-ass_franssen_11454
SEQUENCE=TGCGACTAACTCCAACGACAAACATTTCTCCTATTGGAGGAAGCCTTACAAAAAATCATCACTGATAACCCACATAAAAAATAAAACTCTTATTTATATATATATTTACAGGTCATGTGTCAGATTATATATCATGGCAAGGTTGTTCTATGCTATGTATAGTCTATTACATCTTCAAAATAAGACCTAATGCATGTGTGTTTGATTTTGTGGGACAAACTCGTTTTGTCTTTGTATCTCTATTATGTTTTGACGAGTGAACGTCTTTTGTTTTATGTATTTAAAGTCG
TARGET=95,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2694
PRIMER_LEFT_PENALTY=0.088662
PRIMER_RIGHT_PENALTY=0.180765
PRIMER_LEFT_SEQUENCE=TCCTATTGGAGGAAGCCTTACA
PRIMER_RIGHT_SEQUENCE=AAGACGTTCACTCGTCAAAACA
PRIMER_LEFT=28,22
PRIMER_RIGHT=266,22
PRIMER_LEFT_TM=60.089
PRIMER_RIGHT_TM=59.819
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.2694
PRIMER_LEFT_1_PENALTY=0.088662
PRIMER_RIGHT_1_PENALTY=0.180765
PRIMER_LEFT_1_SEQUENCE=TCCTATTGGAGGAAGCCTTACA
PRIMER_RIGHT_1_SEQUENCE=AACAAAAGACGTTCACTCGTCA
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=271,22
PRIMER_LEFT_1_TM=60.089
PRIMER_RIGHT_1_TM=59.819
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.3841
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.180765
PRIMER_LEFT_2_SEQUENCE=CGACTAACTCCAACGACAAACA
PRIMER_RIGHT_2_SEQUENCE=AAGACGTTCACTCGTCAAAACA
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=266,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=59.819
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.3841
PRIMER_LEFT_3_PENALTY=0.203343
PRIMER_RIGHT_3_PENALTY=0.180765
PRIMER_LEFT_3_SEQUENCE=CGACTAACTCCAACGACAAACA
PRIMER_RIGHT_3_SEQUENCE=AACAAAAGACGTTCACTCGTCA
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=271,22
PRIMER_LEFT_3_TM=60.203
PRIMER_RIGHT_3_TM=59.819
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.4065
PRIMER_LEFT_4_PENALTY=0.088662
PRIMER_RIGHT_4_PENALTY=0.317850
PRIMER_LEFT_4_SEQUENCE=TCCTATTGGAGGAAGCCTTACA
PRIMER_RIGHT_4_SEQUENCE=CAAAAGACGTTCACTCGTCAAA
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=269,22
PRIMER_LEFT_4_TM=60.089
PRIMER_RIGHT_4_TM=60.318
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_01246 Ps_contig_mira-and-tgicl-ass_franssen_11473
SEQUENCE=TATTGTAATATTTGCAGCAATTTGCTTACTATCTTTGTAATCTGTGTATCATATCTGTTGTTCATAGTATTTTGTTGTTGGTTATGAAATTTGCAATACAAATAATGATGATGATGATGATGATAATGATGATCTTGATAGATGTCAAACATATAGATAGTGTGTACTTTACTGTTTCTGTTGTGGATGTGAGATGTTTCTTGTTTTCATGTGAAGTTATAAGAAAAATTTTT
TARGET=105,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01247 Ps_contig_mira-and-tgicl-ass_franssen_11504
SEQUENCE=GTTTGCGAGGCGGAGAGGGTTGGAGTTGCTTTGGGTTTTTGCGGTGATAAGCGGATCGTTGCCGGCACGGACGGAGAGTTTATGGTGGTGTGAGGGTAGAGATTGAAGAGGTTGAAGGTGGGTTGAAGGGTGACGGCGACGGAAGTAGGTGGATTTGCCGGTGATGAAAATTGTTGACGGAGGGTTTTTGCACGGAGGAGGTTGTGCGATGGTTTTTTGGATGGAGGAGGGTTTTGTGGTTGTTGACGATGAAAGGTTGGGTTTGTGAGTTGTGGAAGATAGAGGTTATGGGTGGGTGGGTTTGAGGTGGTGATGGAAGGTTTCACGGCGGTGGATTGGTGGTATTTGAAAG
TARGET=289,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3358
PRIMER_LEFT_PENALTY=0.524309
PRIMER_RIGHT_PENALTY=0.811488
PRIMER_LEFT_SEQUENCE=GTGATGAAAATTGTTGACGGAG
PRIMER_RIGHT_SEQUENCE=TTTCAAATACCACCAATCCACC
PRIMER_LEFT=160,22
PRIMER_RIGHT=350,22
PRIMER_LEFT_TM=59.476
PRIMER_RIGHT_TM=60.811
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=1.6385
PRIMER_LEFT_1_PENALTY=0.826973
PRIMER_RIGHT_1_PENALTY=0.811488
PRIMER_LEFT_1_SEQUENCE=GTTTTGTGGTTGTTGACGATGA
PRIMER_RIGHT_1_SEQUENCE=TTTCAAATACCACCAATCCACC
PRIMER_LEFT_1=230,22
PRIMER_RIGHT_1=350,22
PRIMER_LEFT_1_TM=60.827
PRIMER_RIGHT_1_TM=60.811
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=121
PRIMER_PAIR_PENALTY_2=1.9019
PRIMER_LEFT_2_PENALTY=0.254773
PRIMER_RIGHT_2_PENALTY=1.647160
PRIMER_LEFT_2_SEQUENCE=ATGGTGGTGTGAGGGTAGAGAT
PRIMER_RIGHT_2_SEQUENCE=CTTTCAAATACCACCAATCCAC
PRIMER_LEFT_2=81,22
PRIMER_RIGHT_2=351,22
PRIMER_LEFT_2_TM=59.745
PRIMER_RIGHT_2_TM=58.353
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=1.9873
PRIMER_LEFT_3_PENALTY=0.524309
PRIMER_RIGHT_3_PENALTY=1.463021
PRIMER_LEFT_3_SEQUENCE=GTGATGAAAATTGTTGACGGAG
PRIMER_RIGHT_3_SEQUENCE=TTCAAATACCACCAATCCACC
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=349,21
PRIMER_LEFT_3_TM=59.476
PRIMER_RIGHT_3_TM=59.537
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=190
PRIMER_PAIR_PENALTY_4=2.0107
PRIMER_LEFT_4_PENALTY=1.199179
PRIMER_RIGHT_4_PENALTY=0.811488
PRIMER_LEFT_4_SEQUENCE=GTGGTTGTTGACGATGAAAGGT
PRIMER_RIGHT_4_SEQUENCE=TTTCAAATACCACCAATCCACC
PRIMER_LEFT_4=235,22
PRIMER_RIGHT_4=350,22
PRIMER_LEFT_4_TM=61.199
PRIMER_RIGHT_4_TM=60.811
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=116
=
PRIMER_SEQUENCE_ID=SSR_01248 Ps_contig_mira-and-tgicl-ass_franssen_11523
SEQUENCE=TTTTTTCATTCCTGCTGCTCTACAACCTGAACGAAACTACCTGTGANATTTTTCGACCCAATACCAACTACCATTCTTGTCCTGATAGTTTCAAGACGGAGCAAGAATAGCTTTAGTCCATAGCACCAGCTAGGGCACCACTCTGTTACCAGCCCACAATCCTTTTTGCCATCCCTGCTGCTCTACAACCTGAACGAAACTACCTGTGATAWTTTTTCGACCCAATACCAACTACCATTCTTGTCCTGATAGTTTCAAGAGGAGCAAGAATAGCTTTAGTCATAGCACCAGCTAGGGCACCACTCATAAACTCCCGAACCTCTCTCTTATCTAAAAATTCCTGTGCTTGGCGTGGAAGTTGAAGTCGAAAATGGAGATTAGGGTTTGGAAGAACGTCAATGTCTTTAAGGATAGTGTAAACATCACCAGGGAGCGGTGAGATTGAAGAAGAAGAAGACAGGGTATTAGTTTTTGTGTTTGAAGACATTCTCGGTTTATCCAAAATTTCTTCTTCTTCTTCAACTTATTGTCTTCTTCTTCTCGCTTTCAATAATCGTTTGCGATATAGTACTGTATATGCTTTGGGTTTGAGTTTTGAATTATCACTTGATTCGGCGTAAATTTAACATAATCTGAAACCCTAGGAGCTGTTTCATACTTCTACACTTTGTTCATGTTCATGTCATCCC
TARGET=444,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01249 Ps_contig_mira-and-tgicl-ass_franssen_11523
SEQUENCE=TTTTTTCATTCCTGCTGCTCTACAACCTGAACGAAACTACCTGTGANATTTTTCGACCCAATACCAACTACCATTCTTGTCCTGATAGTTTCAAGACGGAGCAAGAATAGCTTTAGTCCATAGCACCAGCTAGGGCACCACTCTGTTACCAGCCCACAATCCTTTTTGCCATCCCTGCTGCTCTACAACCTGAACGAAACTACCTGTGATAWTTTTTCGACCCAATACCAACTACCATTCTTGTCCTGATAGTTTCAAGAGGAGCAAGAATAGCTTTAGTCATAGCACCAGCTAGGGCACCACTCATAAACTCCCGAACCTCTCTCTTATCTAAAAATTCCTGTGCTTGGCGTGGAAGTTGAAGTCGAAAATGGAGATTAGGGTTTGGAAGAACGTCAATGTCTTTAAGGATAGTGTAAACATCACCAGGGAGCGGTGAGATTGAAGAAGAAGAAGACAGGGTATTAGTTTTTGTGTTTGAAGACATTCTCGGTTTATCCAAAATTTCTTCTTCTTCTTCAACTTATTGTCTTCTTCTTCTCGCTTTCAATAATCGTTTGCGATATAGTACTGTATATGCTTTGGGTTTGAGTTTTGAATTATCACTTGATTCGGCGTAAATTTAACATAATCTGAAACCCTAGGAGCTGTTTCATACTTCTACACTTTGTTCATGTTCATGTCATCCC
TARGET=506,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01250 Ps_contig_mira-and-tgicl-ass_franssen_11523
SEQUENCE=TTTTTTCATTCCTGCTGCTCTACAACCTGAACGAAACTACCTGTGANATTTTTCGACCCAATACCAACTACCATTCTTGTCCTGATAGTTTCAAGACGGAGCAAGAATAGCTTTAGTCCATAGCACCAGCTAGGGCACCACTCTGTTACCAGCCCACAATCCTTTTTGCCATCCCTGCTGCTCTACAACCTGAACGAAACTACCTGTGATAWTTTTTCGACCCAATACCAACTACCATTCTTGTCCTGATAGTTTCAAGAGGAGCAAGAATAGCTTTAGTCATAGCACCAGCTAGGGCACCACTCATAAACTCCCGAACCTCTCTCTTATCTAAAAATTCCTGTGCTTGGCGTGGAAGTTGAAGTCGAAAATGGAGATTAGGGTTTGGAAGAACGTCAATGTCTTTAAGGATAGTGTAAACATCACCAGGGAGCGGTGAGATTGAAGAAGAAGAAGACAGGGTATTAGTTTTTGTGTTTGAAGACATTCTCGGTTTATCCAAAATTTCTTCTTCTTCTTCAACTTATTGTCTTCTTCTTCTCGCTTTCAATAATCGTTTGCGATATAGTACTGTATATGCTTTGGGTTTGAGTTTTGAATTATCACTTGATTCGGCGTAAATTTAACATAATCTGAAACCCTAGGAGCTGTTTCATACTTCTACACTTTGTTCATGTTCATGTCATCCC
TARGET=530,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01251 Ps_contig_mira-and-tgicl-ass_franssen_11528
SEQUENCE=CAGCTGCCACGGCAGCCGATGAATCAAACCCGTATGCTGAGGGTTTTCTTACTGGAGAGGAAGGTGTGTTGTTGGATGGATAACTTGCGCGAAGGTGGTTCATGTTCATGTTTTGACGCATCTGCATCGCAGTGGGAGATTGCATCTGTTGTGTGGGTGTTGAAGGTTGCATGGAAAGACCATGTAGTTGAGATAGTAAATTAGGATCAGCAGATGCTAATAGGTCATCGAGGTTAGTTGGATTCATATCAACAATCCTGTTGAATTCGTTGCTCCGGAAAGATGGAGAAGAGATTCTGGCAATCTCTTCAATCATTTGCTGTTGTTGTTGTTGCTGCTGTTGCTGCTGGTGCTGTTGTTTGACGGCGAGGACTATCGAGACCGAGCAG
TARGET=321,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9970
PRIMER_LEFT_PENALTY=0.069737
PRIMER_RIGHT_PENALTY=1.927237
PRIMER_LEFT_SEQUENCE=ATCTGTTGTGTGGGTGTTGAAG
PRIMER_RIGHT_SEQUENCE=CTGCTCGGTCTCGATAGTCCT
PRIMER_LEFT=143,22
PRIMER_RIGHT=388,21
PRIMER_LEFT_TM=59.930
PRIMER_RIGHT_TM=60.927
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=57.143
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=2.0595
PRIMER_LEFT_1_PENALTY=0.132262
PRIMER_RIGHT_1_PENALTY=1.927237
PRIMER_LEFT_1_SEQUENCE=GGTGGTTCATGTTCATGTTTTG
PRIMER_RIGHT_1_SEQUENCE=CTGCTCGGTCTCGATAGTCCT
PRIMER_LEFT_1=93,22
PRIMER_RIGHT_1=388,21
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=60.927
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=57.143
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=2.0963
PRIMER_LEFT_2_PENALTY=0.069737
PRIMER_RIGHT_2_PENALTY=2.026532
PRIMER_LEFT_2_SEQUENCE=ATCTGTTGTGTGGGTGTTGAAG
PRIMER_RIGHT_2_SEQUENCE=CTGCTCGGTCTCGATAGTCC
PRIMER_LEFT_2=143,22
PRIMER_RIGHT_2=388,20
PRIMER_LEFT_2_TM=59.930
PRIMER_RIGHT_2_TM=59.973
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=2.0963
PRIMER_LEFT_3_PENALTY=0.069737
PRIMER_RIGHT_3_PENALTY=2.026532
PRIMER_LEFT_3_SEQUENCE=ATCTGTTGTGTGGGTGTTGAAG
PRIMER_RIGHT_3_SEQUENCE=TGCTCGGTCTCGATAGTCCT
PRIMER_LEFT_3=143,22
PRIMER_RIGHT_3=387,20
PRIMER_LEFT_3_TM=59.930
PRIMER_RIGHT_3_TM=59.973
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=2.1034
PRIMER_LEFT_4_PENALTY=0.069737
PRIMER_RIGHT_4_PENALTY=2.033680
PRIMER_LEFT_4_SEQUENCE=ATCTGTTGTGTGGGTGTTGAAG
PRIMER_RIGHT_4_SEQUENCE=CTCGGTCTCGATAGTCCTCG
PRIMER_LEFT_4=143,22
PRIMER_RIGHT_4=385,20
PRIMER_LEFT_4_TM=59.930
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_01252 Ps_contig_mira-and-tgicl-ass_franssen_11547
SEQUENCE=CGGCCTGCAATGTTGTTGGAATGATCGATCGTAACTTATTGGGTATACATCATCTCTACCGGAGACCGATATATGCGCGGATGCCTGAATGTAGTATCAATTCTCCTGACTATGGACCATTGCCTCCTGATGCTCCTTCTTGGTGTCAAGCTCCTTTCGATCCCGAAGGACTCTTAAGTTCAGTGATGGCTATTGTTACCTGCTTGATTGGCTTGCATTATGGTCACATTATTCTCCATTTTAAGGATCATAGAATTAGAATCATATACTGGATGATTCCAACCTCTTGTCTTGTAGTTTCTGGTCTTGTCTTGGATTTATTTGGAATGCATGTAAATAAAGTTCTCTACTCATTGAGTTATACTTGTCTCACTGCTGGTGCTTCTGGCATCCTCTTTGTTGGAATATACCTGATGGTTGATGTATGTGGCTATAGTCGCATGACTTCGATTTTGGAATGGATGGGGATGCATGCATTAATGATATATGTCCTAGCAGCTTGCAACATTTTCCCAATTTTCATCCAAGGATTTTATTGGGGAAATCCTCATAATAACCTTCTAAAGTTAATTGGAGTTGGAACATGAAGGGGGAGCAAATATTGCTAGTGGACAAGAAAATTATTGTGTTGTTAAATGGTGAGACATTTTTTTGTTACACCTATGTCTTAGAGTAACGAGGAGGACCAAGCAATGTTTGTGGATTTTTGAGACAAATTCTGAGCTGGTCTTTTTGTTCAAGAACATGAACATGAATAATGTTTCTCTGATCTATTGATAAAGTCACCTACATGGATGATGCATGATGTATTTTGGAGAAGATACATTTCTTCATGGCATAGCCTTTTTTTAATTTTTATATATAGAAAAAAATGAGCAGCTTTTGTTTATAGTATATGACTGCTAAATATTTTGTTACTTTGTATCCTTGAAATGGTAGATTTCAAGATGAGATATCAATGTACAACTTTTTTTATTATTATTATTATTAGTNTAAA
TARGET=973,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01253 Ps_contig_mira-and-tgicl-ass_franssen_11557
SEQUENCE=GTTTTTGTATCATCATCAATAGAAAGAGTAAAGTAGAAGAATCTCCAACTTGAGAAGTGCTTGTTGATCTTGAATACTTTCTTTGAAGAAGATGAAGATGTTGTTGATGAAGATGATGATGATGATGAAT
TARGET=112,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01254 Ps_contig_mira-and-tgicl-ass_franssen_11562
SEQUENCE=CTTCTTACCCAATCAACTTCAATTAAACATAAACTTCAACACACACAAAACTGATACCTTTACTATACAGATATGAAAATTACAAAATGGAAAATAAAATCTAAGAAATATAAAAACTCATGAACAAAGCTTTGGCAAAGATTTACCATAACTATTAGATGCTTTTGATTTGGTCATCTTAGCCATCTGACTTTGCATTTTCCTACAGATTTTCTCTAACTCCATAACCCTCCATTGCATTCCTTGCAAATGTGCTTTGAGTTTCTCATTATCAGGTGGAATATCAAAATTGCTAGCATACAACACAACTAGTTGTTCATTGTCCTTTTCTTTCTTTCCCTTTTGAGATGAACCGCACGATGAATCGCTGTACGGAGTTGAAATCGGAGTAGTGGCATTGAGTTAAATTTTTTAGCTTCGATTGCTGAGAAACAAGAGCTTGAACCGCGGATTTTGATGGAAATTTTGTGTTCTTCGAAAGGTGTAAGCATGCTTGAGGTGAAAGCTTCTCATAATTTAAACCATAGCATATCTTTATTCTTTCTTCTTGTGATAATTGAGTATGAACCTCAAGATACATGTCCATTGCATGGTAGAGCTCATCACAAGAATCCCTAGCAGAATCAGGTAGAGCTGTGGCAAGAGACCAAAAACTTTGTAAGTTTTTAAACAAGGA
TARGET=327,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2063
PRIMER_LEFT_PENALTY=0.203971
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=TAACTCCATAACCCTCCATTGC
PRIMER_RIGHT_SEQUENCE=CCACTACTCCGATTTCAACTCC
PRIMER_LEFT=216,22
PRIMER_RIGHT=394,22
PRIMER_LEFT_TM=60.204
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.2295
PRIMER_LEFT_1_PENALTY=0.203971
PRIMER_RIGHT_1_PENALTY=0.025566
PRIMER_LEFT_1_SEQUENCE=TAACTCCATAACCCTCCATTGC
PRIMER_RIGHT_1_SEQUENCE=ACTCAATGCCACTACTCCGATT
PRIMER_LEFT_1=216,22
PRIMER_RIGHT_1=402,22
PRIMER_LEFT_1_TM=60.204
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.2295
PRIMER_LEFT_2_PENALTY=0.203971
PRIMER_RIGHT_2_PENALTY=0.025566
PRIMER_LEFT_2_SEQUENCE=TAACTCCATAACCCTCCATTGC
PRIMER_RIGHT_2_SEQUENCE=AACTCAATGCCACTACTCCGAT
PRIMER_LEFT_2=216,22
PRIMER_RIGHT_2=403,22
PRIMER_LEFT_2_TM=60.204
PRIMER_RIGHT_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.2295
PRIMER_LEFT_3_PENALTY=0.203971
PRIMER_RIGHT_3_PENALTY=0.025566
PRIMER_LEFT_3_SEQUENCE=TAACTCCATAACCCTCCATTGC
PRIMER_RIGHT_3_SEQUENCE=GAGAAGCTTTCACCTCAAGCAT
PRIMER_LEFT_3=216,22
PRIMER_RIGHT_3=510,22
PRIMER_LEFT_3_TM=60.204
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=0.2295
PRIMER_LEFT_4_PENALTY=0.203971
PRIMER_RIGHT_4_PENALTY=0.025566
PRIMER_LEFT_4_SEQUENCE=TAACTCCATAACCCTCCATTGC
PRIMER_RIGHT_4_SEQUENCE=ATGAGAAGCTTTCACCTCAAGC
PRIMER_LEFT_4=216,22
PRIMER_RIGHT_4=512,22
PRIMER_LEFT_4_TM=60.204
PRIMER_RIGHT_4_TM=60.026
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_01255 Ps_contig_mira-and-tgicl-ass_franssen_11563
SEQUENCE=AACCAAATGAGCAGTTTTCTAAATCAATACTCGATGCCTTTTTTTTTAAAAAACTAAGTTTTAATCTTCAGAACCTGTTTAAGTTGCTCTCCTAAATATGCTTGGATCTTCAAAAAACTGTCGTTCCCGGTATGTTGGCTTCTTGTAAGTGAAAGGAATCACGAAGTCACTGGTGGTTGGCCATCGCCGCCTTCGTGGGGAGGTGCTATGAATCCGTGAGAGAGGGAGGCACTACGTATCCGTGATGGAGGGAGAGAGAGAAAGAGAGAGAGAGAGAGAGAGAGTGTGYGGGTGGGTGTNTCTCTKAGTGG
TARGET=252,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01256 Ps_contig_mira-and-tgicl-ass_franssen_11563
SEQUENCE=AACCAAATGAGCAGTTTTCTAAATCAATACTCGATGCCTTTTTTTTTAAAAAACTAAGTTTTAATCTTCAGAACCTGTTTAAGTTGCTCTCCTAAATATGCTTGGATCTTCAAAAAACTGTCGTTCCCGGTATGTTGGCTTCTTGTAAGTGAAAGGAATCACGAAGTCACTGGTGGTTGGCCATCGCCGCCTTCGTGGGGAGGTGCTATGAATCCGTGAGAGAGGGAGGCACTACGTATCCGTGATGGAGGGAGAGAGAGAAAGAGAGAGAGAGAGAGAGAGAGTGTGYGGGTGGGTGTNTCTCTKAGTGG
TARGET=263,22
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01257 Ps_contig_mira-and-tgicl-ass_franssen_11573
SEQUENCE=CCGTCGGGGTATTKATCAACAATCATAATGYTAAATTAAAACCTCTCTCTCTCTCCTCTCTCTTTCTCTCTCTACTACTTGTTCTTCACTCACTACCATGGCTTTCTGGTTTAACCATGGCCGTTAGATCTTTGATCACTCTAATGTGAGTGAATCAATGTTACTTCTGACTAGAAAAAACAAACACCTTGCCATTGGATCCAACTCAGTGGATAATATTGNGGAA
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01258 Ps_contig_mira-and-tgicl-ass_franssen_11599
SEQUENCE=GTGACTGGCATCGATTTAACCAAACGAGAGGCATATTTATCTCAAGAAGAGTTCCACGAAAAGTTCGGAATGCCAAAGTCTTCCTTCTATAAACTCCCTAGATGGAAACAAAACAAGCTGAAGATTTCACTGGATCTATTTTAGAGAATGAACAACAAATGTCTGTGTTTTAATTGTGGCACTATATATATAACATGATACAATTTTATCTCTTGAGTGTGTATTATGCACGCGTGAATAGCTTACTACAGATTTTTATTCTTTCATTTTGTTTTATGATAATATACTTTTGTGATGATGCTTTTGGGGC
TARGET=183,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4517
PRIMER_LEFT_PENALTY=0.243965
PRIMER_RIGHT_PENALTY=1.207756
PRIMER_LEFT_SEQUENCE=ACTGGCATCGATTTAACCAAAC
PRIMER_RIGHT_SEQUENCE=GCGTGCATAATACACACTCAAGA
PRIMER_LEFT=3,22
PRIMER_RIGHT=232,23
PRIMER_LEFT_TM=60.244
PRIMER_RIGHT_TM=60.208
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=1.7015
PRIMER_LEFT_1_PENALTY=0.493727
PRIMER_RIGHT_1_PENALTY=1.207756
PRIMER_LEFT_1_SEQUENCE=GATTTAACCAAACGAGAGGCAT
PRIMER_RIGHT_1_SEQUENCE=GCGTGCATAATACACACTCAAGA
PRIMER_LEFT_1=12,22
PRIMER_RIGHT_1=232,23
PRIMER_LEFT_1_TM=59.506
PRIMER_RIGHT_1_TM=60.208
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=1.7061
PRIMER_LEFT_2_PENALTY=0.498341
PRIMER_RIGHT_2_PENALTY=1.207756
PRIMER_LEFT_2_SEQUENCE=ATCGATTTAACCAAACGAGAGG
PRIMER_RIGHT_2_SEQUENCE=GCGTGCATAATACACACTCAAGA
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=232,23
PRIMER_LEFT_2_TM=59.502
PRIMER_RIGHT_2_TM=60.208
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=1.7618
PRIMER_LEFT_3_PENALTY=0.243965
PRIMER_RIGHT_3_PENALTY=1.517800
PRIMER_LEFT_3_SEQUENCE=ACTGGCATCGATTTAACCAAAC
PRIMER_RIGHT_3_SEQUENCE=ACGCGTGCATAATACACACTCA
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=234,22
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=61.518
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=232
PRIMER_PAIR_PENALTY_4=1.8458
PRIMER_LEFT_4_PENALTY=0.243965
PRIMER_RIGHT_4_PENALTY=1.601882
PRIMER_LEFT_4_SEQUENCE=ACTGGCATCGATTTAACCAAAC
PRIMER_RIGHT_4_SEQUENCE=GCGTGCATAATACACACTCAAG
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=232,22
PRIMER_LEFT_4_TM=60.244
PRIMER_RIGHT_4_TM=58.398
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_01259 Ps_contig_mira-and-tgicl-ass_franssen_11624
SEQUENCE=CAACTTTGTCTACATTTTATCATTCCGAGTAGTTTGGTACCTACCTTGTACTGGTTGCTAGACTGCTTCTGCCTGCTGGCATTAATGATACCATAGCTACATACCAAGAGGGAACCTGGTRGCCTTGCTACCATGAAAACTCAGCTTACAAGGGCATGTTATGGTCCAATCCTGCTACACCAGCAACAACAACAACAATTACACCAGCAGCAACAACAACAGTTATACAACACCATTGACTTCACTCAGAACAAGCATCACCATGGCAGGCTCATTCCATCCTAAACCAGATCAATGCATAATGACTAGAGTTGGAACATTTGACTTGAGCTAAGTCATATCAACCAAGAA
TARGET=184,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01260 Ps_contig_mira-and-tgicl-ass_franssen_11631
SEQUENCE=GTSGGGGACACACAAAATAGTATTCAATATTTCATATCAACCAGCAGCTAGCTACTTATTGATTCATGGGCAGAACAAACACGTTGATGCATCTCCGGAGGTCTGCTAAGGGAGCGGAGAGTACTGCCGGGCTCTGCAAATTGATCTTAGGGAAGGGAAGCGTGGGTCGCAGCGACCGGGTTGATACAAAAGACAAAGAGTAGTAGTAGTAGGGAAGAAGGCAG
TARGET=199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01261 Ps_contig_mira-and-tgicl-ass_franssen_11645
SEQUENCE=CAGCCACACCAACATAGAAACATGTTCCAACCCCAAAGTTCACCTTGCAAACTGAGCTTAAAATAATTTCCATGATGACAAAGTACAAGTCCAATGAGACCAATAATGAGAAAAGGGAAATTGGAAATCACATCAAGTGCATTGGGAATACCGAAAAAGGTGCGTTGATCGGCGAAATTGTGATAATCTTGGGACTGAGGAATGGATGGGGTTAGAATCATGAGTACTATGAAACAAACGAGAGCTACAGCCAGAGAATAAGCGGTGCGTTTTCTCATTTCTGTTTGATTGATGAAGTCGGAGAGAAAAGAAAAGAATATGAGATCAGAGAGGAGGAGGAGGAGGGAAAGGGCGTGTGTAGTGCGGTCCCCGC
TARGET=330,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01262 Ps_contig_mira-and-tgicl-ass_franssen_11660
SEQUENCE=TCGGGGTCCCTTCTTCAAGTTCAGTTTCTCCTATGCCGCGCCAGATGTCGCCTCCGGCGGCGGCGGAGCCTCAATGTCCAAAATTAACAGATAAACTTCTTCAATTCTTTCCCCTAAACCGAATATCAAAGTAGAACAAACCAATTCTCCATGAATAACCTGAATTGAAGCATAGAGTTGAAGAACCCTAGATCCTGATATGCAAAATTAAATCTCTCTCCCTCGGAATTAAAGCCCTAGAGGTTAGGGATTTGATTCCCCTAACCTCCCTCTATATTCCGGTAATTCACACATAACGAAATAATGAAGATGACGAAAAACTCACCGGAGCAGCGCCAGAATCAGGACCGTGGCATGTTGTGTTGATGCAATGGCTTGTTGCTCATGCTGTTGTGGAATGGTTATTATAGGGTTTATGTATGGTAGGTTTGGTTGGATCCATGGTTTGCTGTAGATTTATTGTTACATGGTTGCAGGTTAATTGATGTCGGATGGTACATGGCCATGTTGTTGAAATATTGATGCAAAATTCTAGGTATAGTATTTACTGCAAGATAGTATGGGATGCTCATGTTGTTGTGTGACTGTTGTAGGCCGATATGAGGTGGTGGACAAAGGCTTTGGCGTGTT
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2283
PRIMER_LEFT_PENALTY=0.216588
PRIMER_RIGHT_PENALTY=0.011726
PRIMER_LEFT_SEQUENCE=AAGTTCAGTTTCTCCTATGCCG
PRIMER_RIGHT_SEQUENCE=AACCTCTAGGGCTTTAATTCCG
PRIMER_LEFT=16,22
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=59.783
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.7000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.4602
PRIMER_LEFT_1_PENALTY=0.216588
PRIMER_RIGHT_1_PENALTY=0.243575
PRIMER_LEFT_1_SEQUENCE=AAGTTCAGTTTCTCCTATGCCG
PRIMER_RIGHT_1_SEQUENCE=TCGTTATGTGTGAATTACCGGA
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=298,22
PRIMER_LEFT_1_TM=59.783
PRIMER_RIGHT_1_TM=60.244
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.7000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.5317
PRIMER_LEFT_2_PENALTY=0.216588
PRIMER_RIGHT_2_PENALTY=0.315112
PRIMER_LEFT_2_SEQUENCE=AAGTTCAGTTTCTCCTATGCCG
PRIMER_RIGHT_2_SEQUENCE=GGGAATCAAATCCCTAACCTCT
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=59.783
PRIMER_RIGHT_2_TM=59.685
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.5848
PRIMER_LEFT_3_PENALTY=0.216588
PRIMER_RIGHT_3_PENALTY=0.368179
PRIMER_LEFT_3_SEQUENCE=AAGTTCAGTTTCTCCTATGCCG
PRIMER_RIGHT_3_SEQUENCE=TAGGGGAATCAAATCCCTAACC
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=59.783
PRIMER_RIGHT_3_TM=60.368
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.6520
PRIMER_LEFT_4_PENALTY=0.216588
PRIMER_RIGHT_4_PENALTY=0.435429
PRIMER_LEFT_4_SEQUENCE=AAGTTCAGTTTCTCCTATGCCG
PRIMER_RIGHT_4_SEQUENCE=ACCTCTAGGGCTTTAATTCCGA
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=243,22
PRIMER_LEFT_4_TM=59.783
PRIMER_RIGHT_4_TM=60.435
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=11.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_01263 Ps_contig_mira-and-tgicl-ass_franssen_11664
SEQUENCE=CGTTGGTGGTTCTGGTGGTGGTGTCTTTCCTCATCCTTGAGTAACAAGATAATTACTATATATGGAATTTACTTTATTTTAGTATTATTAATAAAATAAAAGTTAATTATATTGGGTTTTATTGTTATCACAATTGTTTAATTTTAGTGTTCTATTATTATATTTTTCACCTACAGAAACTGTGTTAGCTAGTACTATTTTGTTTTGTTATGATGTTTATGATGTGTTGTTCTTTCTAATGTAATGTAATTGTATGGGATATACATTTCAYCATCTACAAGTTTATCAGTTAAAACCGAAAATTTATGTATATATATATGAAATATTATATATTTT
TARGET=309,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01264 Ps_contig_mira-and-tgicl-ass_franssen_11677
SEQUENCE=CTTTGTCTTTTGTGATAACTAGCTGACTAGGGATGCCAACCTTGCCGTAGCTCTCAGATAAGTTTAGATCCACCACATCTTGAAGCATGGATTGGCGATTGTGTAAATGTTGAATTGCATCTGGGGGATCAAGAACAATTACATCGGGATGTTTTTGCCTGAAATCCTCGATAATATCACGCCATTCCTCTCCTGACAACTTATGCAAGACAATATCAAATGGACCTTGTTCTGACAGTGGTTTGTTCACATCAATAGCAACAAAGTATATGCCCTTGCTCCTTGCAAGAGAAATGAAATTCGGTTGCAGAAAGCTTTTCTTCTTCTTAGCCGTTAACGCATACCCAACAACAACTTTCTGTCCCGAAAACGGCGTCGTACCGACCTCAGATTTCTCCATTACTTCTTCTTCTTCGCCGCTCGAAAACTCTCCGTTTAGCCTCATACCGAAAACACCCCTGATTCAAAGATCAAATCTTTTTCCCTTTTCCAATCAATTCACCTCAAAATCAATACGAAATCGAAAAACTTGAGAGTCAAAAACAAGATTATGAAAATTGAAATTGAGAAAGGGAATTTCGTTAGTGAAATTGGTGTAATGTGTGAGTAATTTATGATGAATTTATAGGAAAACAAAAGAGGAATATTCAGTTTGCAGAGAGAAGAAAAAGTGTGGATTGGGTTTTTTTAGAATGAACGAAATGCCCGCTTACTCTGCGTTGATA
TARGET=403,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2228
PRIMER_LEFT_PENALTY=0.199358
PRIMER_RIGHT_PENALTY=0.023406
PRIMER_LEFT_SEQUENCE=TTAACGCATACCCAACAACAAC
PRIMER_RIGHT_SEQUENCE=GTTTTCGGTATGAGGCTAAACG
PRIMER_LEFT=333,22
PRIMER_RIGHT=453,22
PRIMER_LEFT_TM=59.801
PRIMER_RIGHT_TM=60.023
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.2228
PRIMER_LEFT_1_PENALTY=0.199358
PRIMER_RIGHT_1_PENALTY=0.023406
PRIMER_LEFT_1_SEQUENCE=GTTAACGCATACCCAACAACAA
PRIMER_RIGHT_1_SEQUENCE=GTTTTCGGTATGAGGCTAAACG
PRIMER_LEFT_1=332,22
PRIMER_RIGHT_1=453,22
PRIMER_LEFT_1_TM=59.801
PRIMER_RIGHT_1_TM=60.023
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=0.2242
PRIMER_LEFT_2_PENALTY=0.038361
PRIMER_RIGHT_2_PENALTY=0.185822
PRIMER_LEFT_2_SEQUENCE=TACCGACCTCAGATTTCTCCAT
PRIMER_RIGHT_2_SEQUENCE=GAGGTGAATTGATTGGAAAAGG
PRIMER_LEFT_2=378,22
PRIMER_RIGHT_2=504,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=59.814
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=127
PRIMER_PAIR_PENALTY_3=0.3071
PRIMER_LEFT_3_PENALTY=0.199358
PRIMER_RIGHT_3_PENALTY=0.107763
PRIMER_LEFT_3_SEQUENCE=TTAACGCATACCCAACAACAAC
PRIMER_RIGHT_3_SEQUENCE=TGAGGCTAAACGGAGAGTTTTC
PRIMER_LEFT_3=333,22
PRIMER_RIGHT_3=443,22
PRIMER_LEFT_3_TM=59.801
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=111
PRIMER_PAIR_PENALTY_4=0.3071
PRIMER_LEFT_4_PENALTY=0.199358
PRIMER_RIGHT_4_PENALTY=0.107763
PRIMER_LEFT_4_SEQUENCE=GTTAACGCATACCCAACAACAA
PRIMER_RIGHT_4_SEQUENCE=TGAGGCTAAACGGAGAGTTTTC
PRIMER_LEFT_4=332,22
PRIMER_RIGHT_4=443,22
PRIMER_LEFT_4_TM=59.801
PRIMER_RIGHT_4_TM=59.892
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=112
=
PRIMER_SEQUENCE_ID=SSR_01265 Ps_contig_mira-and-tgicl-ass_franssen_11708
SEQUENCE=ATTATCCTGTTTTCGAATAGCGAGCCAATTTCCACTCAGTTTGCTTGGACTTTGGCTTCTTGAGCTTGGAAAATCCATCATCATCATCATCGGATTCATCGCTATCATCACCCTTCTTCTTGCCGCGTTTCTTCTCTGCTTTTCCATATACAATAATCTTCCTTTCATCTTTCTCCTCAACGGGCTCGGATCTCTCCCTGGGCTTCCAAATGAATAGAGATCTAGAACTTCCAGTAGCTAGGATATCATCGCGTGGATGAAGCTTATTCACCGGA
TARGET=76,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1374
PRIMER_LEFT_PENALTY=0.048692
PRIMER_RIGHT_PENALTY=0.088662
PRIMER_LEFT_SEQUENCE=CACTCAGTTTGCTTGGACTTTG
PRIMER_RIGHT_SEQUENCE=CTCTATTCATTTGGAAGCCCAG
PRIMER_LEFT=32,22
PRIMER_RIGHT=218,22
PRIMER_LEFT_TM=59.951
PRIMER_RIGHT_TM=60.089
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.1565
PRIMER_LEFT_1_PENALTY=0.048692
PRIMER_RIGHT_1_PENALTY=0.107763
PRIMER_LEFT_1_SEQUENCE=CACTCAGTTTGCTTGGACTTTG
PRIMER_RIGHT_1_SEQUENCE=ATGGAAAAGCAGAGAAGAAACG
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=146,22
PRIMER_LEFT_1_TM=59.951
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=0.2828
PRIMER_LEFT_2_PENALTY=0.048692
PRIMER_RIGHT_2_PENALTY=0.234069
PRIMER_LEFT_2_SEQUENCE=CACTCAGTTTGCTTGGACTTTG
PRIMER_RIGHT_2_SEQUENCE=CGTTGAGGAGAAAGATGAAAGG
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=181,22
PRIMER_LEFT_2_TM=59.951
PRIMER_RIGHT_2_TM=60.234
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=0.2828
PRIMER_LEFT_3_PENALTY=0.048692
PRIMER_RIGHT_3_PENALTY=0.234069
PRIMER_LEFT_3_SEQUENCE=CACTCAGTTTGCTTGGACTTTG
PRIMER_RIGHT_3_SEQUENCE=CCGTTGAGGAGAAAGATGAAAG
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=182,22
PRIMER_LEFT_3_TM=59.951
PRIMER_RIGHT_3_TM=60.234
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.3039
PRIMER_LEFT_4_PENALTY=0.215215
PRIMER_RIGHT_4_PENALTY=0.088662
PRIMER_LEFT_4_SEQUENCE=CAATTTCCACTCAGTTTGCTTG
PRIMER_RIGHT_4_SEQUENCE=CTCTATTCATTTGGAAGCCCAG
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=218,22
PRIMER_LEFT_4_TM=59.785
PRIMER_RIGHT_4_TM=60.089
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_01266 Ps_contig_mira-and-tgicl-ass_franssen_11729
SEQUENCE=AATGCCTAATAGAAGATTGTCAAAAGATCCTGAATCTATGTTCAATATAGAAGCCAAGCCAGCACCTACAAAAGACCCTATTGTTGTTCCCAAGTTGTTTATTGACATGAAAAGAGCAAATAATGTCCCTTCAATTCCTGGAGGGCATAGTCGTCCAGATAAGATCAGGAAAGGCATAAACTTGAATTGATTGATTGCATCAACAAGAGCTGAGCCAAAGAGCACCATAACCCTGTCGGATACTCCAAAGGCAATATTTTTTCTAGACACAACAGCAATCTGCAGAAGATTCAAGAAGGCCAACGCGACATGTGCGTACATGAGAATTCTTCGTAGCGTCATATGCTTTAGGTGACGGTTATAAATGAATGTTCCAAGCATAAGTWCCTAACCATCCAAACAACACGTCG
TARGET=186,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01267 Ps_contig_mira-and-tgicl-ass_franssen_11769
SEQUENCE=CTCCAAAAATCAGACCAAATTAACCCATAAACCCTCAAATAAACAACATCCACAACATGAAATCACGAGAAAAATAACATATCAACATGAAAACAAGTCAAACATAGTTCAAATCAAGCAATCAACATTAGGTTTTACACCAAGCCTTAGTTTAATTCCAAACACAACAATATAAGCATCAAACAAACAAAAGGATTTCATACTACTAATAAGAAAGAATAATTGTTAGTGTCCAAGAAGTTACTAGGAGTGAAGGCTCCATAAGGCTTGGGT
TARGET=180,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3997
PRIMER_LEFT_PENALTY=0.140832
PRIMER_RIGHT_PENALTY=0.258908
PRIMER_LEFT_SEQUENCE=ATCCACAACATGAAATCACGAG
PRIMER_RIGHT_SEQUENCE=CCTTATGGAGCCTTCACTCCTA
PRIMER_LEFT=47,22
PRIMER_RIGHT=265,22
PRIMER_LEFT_TM=59.859
PRIMER_RIGHT_TM=59.741
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=1.1827
PRIMER_LEFT_1_PENALTY=0.923750
PRIMER_RIGHT_1_PENALTY=0.258908
PRIMER_LEFT_1_SEQUENCE=CCACAACATGAAATCACGAGAA
PRIMER_RIGHT_1_SEQUENCE=CCTTATGGAGCCTTCACTCCTA
PRIMER_LEFT_1=49,22
PRIMER_RIGHT_1=265,22
PRIMER_LEFT_1_TM=60.924
PRIMER_RIGHT_1_TM=59.741
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=1.2297
PRIMER_LEFT_2_PENALTY=0.140832
PRIMER_RIGHT_2_PENALTY=1.088867
PRIMER_LEFT_2_SEQUENCE=ATCCACAACATGAAATCACGAG
PRIMER_RIGHT_2_SEQUENCE=AAGCCTTATGGAGCCTTCACTC
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=268,22
PRIMER_LEFT_2_TM=59.859
PRIMER_RIGHT_2_TM=61.089
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=1.2784
PRIMER_LEFT_3_PENALTY=0.140832
PRIMER_RIGHT_3_PENALTY=1.137562
PRIMER_LEFT_3_SEQUENCE=ATCCACAACATGAAATCACGAG
PRIMER_RIGHT_3_SEQUENCE=AGCCTTATGGAGCCTTCACTC
PRIMER_LEFT_3=47,22
PRIMER_RIGHT_3=267,21
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=59.862
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=1.4198
PRIMER_LEFT_4_PENALTY=0.140832
PRIMER_RIGHT_4_PENALTY=1.278953
PRIMER_LEFT_4_SEQUENCE=ATCCACAACATGAAATCACGAG
PRIMER_RIGHT_4_SEQUENCE=CCTTATGGAGCCTTCACTCCT
PRIMER_LEFT_4=47,22
PRIMER_RIGHT_4=265,21
PRIMER_LEFT_4_TM=59.859
PRIMER_RIGHT_4_TM=59.721
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_01268 Ps_contig_mira-and-tgicl-ass_franssen_11773
SEQUENCE=ACCTATTGAAACAACCAAAGTTTTACTTTCTAACCCTACCAGTCCTTAGGTCATGTTTAACCGGCCGATCATTTCCAGTAGCTTTCCAGTAACCTCTATTGGTAGCCCTGTTCATTCTTCCACCATTTCCATACTTCTTGTCCAATGCACTAAAAAAATAACCATTCCTGGTCTCTGGTCTTCAATCTTGATTTATCTGATAGGTCCCAGGGCTCACTCTTGTAGATATCAACTTCTGCTATAGCATCAAATCGAAAGGGTTTTCCAAAAAACTTGCGCTTGAGGTAATAAATAACGAGTTCCTCATCAGTTGGGTGAAACCTAAATCCAGGAGCAAGGGAGGTAGGTGGTGCAGCAGTTGCTGAAGCAGACGGCACTGAGACTGAGAGTGGATGGTTCACTAGTGTGGTAGTAGGAGGGGCAGTGAGTAAGAGAGTTTCGTGACCCATGTTGTGTTAGGGCGTGAAGGGAAAAGGGTAGTGATGGTGGTGGTGGGTATGGTGGATTAGGGCTTCGCAGCCGAAGCAAAAAGGAAAAGATGGGTTTTGATTGAGGGTCAAGACAAACCCCCGAGTA
TARGET=484,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1896
PRIMER_LEFT_PENALTY=0.038090
PRIMER_RIGHT_PENALTY=0.151533
PRIMER_LEFT_SEQUENCE=AGAGAGTTTCGTGACCCATGTT
PRIMER_RIGHT_SEQUENCE=GTCTTGACCCTCAATCAAAACC
PRIMER_LEFT=430,22
PRIMER_RIGHT=562,22
PRIMER_LEFT_TM=60.038
PRIMER_RIGHT_TM=59.848
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=0.1896
PRIMER_LEFT_1_PENALTY=0.038090
PRIMER_RIGHT_1_PENALTY=0.151533
PRIMER_LEFT_1_SEQUENCE=AAGAGAGTTTCGTGACCCATGT
PRIMER_RIGHT_1_SEQUENCE=GTCTTGACCCTCAATCAAAACC
PRIMER_LEFT_1=429,22
PRIMER_RIGHT_1=562,22
PRIMER_LEFT_1_TM=60.038
PRIMER_RIGHT_1_TM=59.848
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=0.2084
PRIMER_LEFT_2_PENALTY=0.056887
PRIMER_RIGHT_2_PENALTY=0.151533
PRIMER_LEFT_2_SEQUENCE=GCACTGAGACTGAGAGTGGATG
PRIMER_RIGHT_2_SEQUENCE=GTCTTGACCCTCAATCAAAACC
PRIMER_LEFT_2=373,22
PRIMER_RIGHT_2=562,22
PRIMER_LEFT_2_TM=60.057
PRIMER_RIGHT_2_TM=59.848
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.2508
PRIMER_LEFT_3_PENALTY=0.099308
PRIMER_RIGHT_3_PENALTY=0.151533
PRIMER_LEFT_3_SEQUENCE=TAGGAGGGGCAGTGAGTAAGAG
PRIMER_RIGHT_3_SEQUENCE=GTCTTGACCCTCAATCAAAACC
PRIMER_LEFT_3=412,22
PRIMER_RIGHT_3=562,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=59.848
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.2513
PRIMER_LEFT_4_PENALTY=0.099730
PRIMER_RIGHT_4_PENALTY=0.151533
PRIMER_LEFT_4_SEQUENCE=CACTGAGACTGAGAGTGGATGG
PRIMER_RIGHT_4_SEQUENCE=GTCTTGACCCTCAATCAAAACC
PRIMER_LEFT_4=374,22
PRIMER_RIGHT_4=562,22
PRIMER_LEFT_4_TM=59.900
PRIMER_RIGHT_4_TM=59.848
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_01269 Ps_contig_mira-and-tgicl-ass_franssen_11780
SEQUENCE=GCTTAAATTGGCTATTATTTCGATTGAATGAGAAAGGAGGGATGAAAGTGGAACAAGTTCTTGAAGAGATAGAGAAAGGTAGACAAAAAGCTGAGGGGTGGAAACCTTCTTTAGAGAGCATTGTGGAAGCTCCTGAAGTTCTTGAGATCAATTGATTTTCACACCCTCTCAATTTTTCTTCTTCTTCTTCTTCATGTTTGAGATATAGAGATCCTTTTTGTAATGACTGATTAGATCTCCCACTTTTGTAAATCTTAATTAGGGTCAAGAAATTGTTTAAATTCGATCGAGGAAATAAACTTGTGTTATTTTCTGCTAGTAATTCTTTTCTCCATAAGCTGGATGGAACATATTATTTTCCAGCATCTAGCTCTTACMTT
TARGET=176,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01270 Ps_contig_mira-and-tgicl-ass_franssen_11848
SEQUENCE=TACGCGGGGGRAAAACAGTTGCATTTCATTGCGGTTACCAAAACCTCAATTTGAAAAAATGGCATCCCTTGCTCTTCTCCGTCCCTGCACATTCCACCACACCCACAAGCCTTCTACCCTAACCCTCAAACCAAAACGCAATTCCATTTCATTCTCACCTTTATCAACACCACCATCAAAACTCAAACTCCGAAATGGCGTCGTTAAAGCCAACCTATCCTCGCCCGCACCACCGGAGCGGTTAATCTCAATCGCCGCATACGCACTTCCATTCTTCAATTCCCTTCAATACGGTCACAACATCCTTACACAATACCCTAAATTCGCACTCCTCTTCGATCCAATCATTCCCTTCCTTTCATTCTACAGATCCCTCCCTTACGCCTCGTTTGTCGCATTCTTTGCTCTCTACCTGGGGATAGTGAGAAACCCTAGTTTCCCTCAATTTGTTAGATTCAACGCTATGCAAGCACTCACGCTCGATGTTCTCCTTGTACTTCCGGTTCTGTTTCAGAGGATCTTCTCTCCCGGTCGATATGGATTGGCCTTTAAGGTTATGGTTTGGTCTCACAATGTGGTGTTCGTTTTTAGTATTCTCTGTTTTCTTTATAGTGCTTTGTCTTGTCTTTTGGGTCGGACTCCTTACTTGCCTTTTGTTGCTGATGCTGCTGCCAGACAAATCTGAATATCATTTCTATGTTTATATTTCTTTATTGTATTATCCATAAACAAATATTCTCTTTTGTTTTAATGTTTGTTACTTGATTGGAATGAATGAATACAATTAGGTGTCCTTTTCAATTTTGAACCT
TARGET=769,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01271 Ps_contig_mira-and-tgicl-ass_franssen_11853
SEQUENCE=GGGAAACCGAWTTGATCTCTGTCGAATCGACGCTCTCTCTTACTTCCTCTGCTCTAACTCAACCTTCACTTCACTTCGCTTCGCTTCGACCTAAGCAAACTTTCGCAATGTCTGACAAAGGAGGAAAGGCTGGTTCCCTGTTAAGCAAAGGATCCCTCAAGGGGAAAGACGACAGTGCAACAAAATCAGCAAAGGGAAGGAAGGTTCAGTTTGCCAAAGAAGCTCCATTTGAATCCATACTAAACGGGCAAAAATCTGGTGGAAAAGCTGATTTTGGCAGAGGAAAAGGAGATAAGATTGCAAATGGTGGGAAGAGTTCTGCAACAAAAGACCCGCATCAATTTGAACATAGAGTTGATCAAAAACTTCCTGACAATTTCAAATGCTTGATGGACTGCGAGGCTGCAGTTTTGTTACAAGGAATTCAAGATCGTATGGTTTCGTTATCTAGAGATCCAGCTATCAAAATCCCTGCATCATTTGACAAGGGACTGCACTATGCCAAAAGCAGCAACAAGCATTCAAATCCCGAGTTTGTTAGACTCGCTCTTGAGCCTCTAATGAATCATGGTCTCACTGAGAGCGAGATATGTGTGATTGCTAATGCATACCCCGAAACTGCTGATGAAGTTTTTGCTCTCCTCCCATCACTAAAGGGTAAGAGGGGCATTAACAGCCAACCTATTGAAGAATCATTGAGTGAGCTTGCTAAGCTTAAACAGACTATGTAAACTAAAACCTCATAATGAAAAGGTTCACTGTTTTACTGTCTAGTAAAGCTAGAAGTAACTGCTCTGGCAAATGTAGCACATTCAAAACGCTTGATGAACTCACTAATTGAGACAATATATAATGCAGAACTTTAGTCTGAATATTATATTCGCCTTTTTAGTCTCATGCTATTAGGATGTACATTGGTTTAATGGATCTTATGCATGGCAACAAGGCGAAACTTGGTGACTTTAAGGAATGTTGTATAATCCATTTTGCTGTAGAATATTGTCTCATCACTTCAAATTTTTATAAAACMAGAAAAGGACCATTGTCATGGCACG
TARGET=74,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01272 Ps_contig_mira-and-tgicl-ass_franssen_11854
SEQUENCE=TCGGGGTAGAGGGATGTTCGAATTTCAATTGCATTTCAAAATTATTATTATTATTAGAGCAATGAGCGCTACGCAACTGTTTCACACGGCGCTGCTACCGTCGCTCCGCCCTTGTTGCCGGTGTTATAATAACGTCCGCACAACCTCCACCGCACAGGGGCTGAGACGCGGGTGGGTCCTCCCCAGCACAAACACGAAGAAAGCTGCTGTTGGTTCAAGTAATAATAAAGTGTATGGACG
TARGET=41,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01273 Ps_contig_mira-and-tgicl-ass_franssen_11872
SEQUENCE=ATCCTTTTCACATCAAAATGATCCTTCTTCTCACCTTCCCTAATATTTCCATATAAATACAATTCATGTAACTTTTCACATAATCATTTTGCTATTAACCAAAAACACAACAACCTTAGAATTACTATGGAAGTGATGATTCAAAGTTCAAAGATGGAGACATTTAATTTTAGTAATGGAACAATGAGTTCACCATATCTAAGTCCACCTTCATCACCAAAACGTTTTGGTGAATATTACTTAAGTGCCCCTTCAAGTCCTTCTAGGCTTTCTGAATTATATAGTCAAATTGATTATTTGTCCATAATGGATCAAACATCATCAACCAACAAAAACAACAACGTTGTTGTTGTTGATGATGATGATGTTTATGATCATGATGATGAAGGTGGTGGTTTTGCTTTCTTTGTTAATCACGATGAATCGAAGAATATGTCAACTCGTTCAGCTGAAGAACTTTTTCATGGTGGCAAAATTAAACCTTTTGAAGAAACTAAGGTTGTCGTTGAACCTAGGAAGCAACAAAACAACGTAGGAGTTGGTTTTGATGATGAAAGAAGAGGAAGAGAAAGAGAAAGAACAAAAGGAACAGATTCATCGTTGAATAATTCTGGTAGAAGAGTAACAAGATCACACTCTCCTTATAGAAAATCAAACTACACATTTGAGTTAGAAGAACAGAATTTCCAACAAAAACAACAACCTCGTATGATCAAAGAGGAATCGAAATCATCGAGTTCGAAGGGTTCGAGGAGATGGAAACTGAGTGATTTGTTGTTGTTTACGAAGTGCTTCGGAAGGGAGAGGATCAAACA
TARGET=343,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6923
PRIMER_LEFT_PENALTY=0.267873
PRIMER_RIGHT_PENALTY=0.424392
PRIMER_LEFT_SEQUENCE=AGTCCACCTTCATCACCAAAAC
PRIMER_RIGHT_SEQUENCE=ACAAAGAAAGCAAAACCACCAC
PRIMER_LEFT=201,22
PRIMER_RIGHT=409,22
PRIMER_LEFT_TM=60.268
PRIMER_RIGHT_TM=60.424
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.8323
PRIMER_LEFT_1_PENALTY=0.407941
PRIMER_RIGHT_1_PENALTY=0.424392
PRIMER_LEFT_1_SEQUENCE=TCTAAGTCCACCTTCATCACCA
PRIMER_RIGHT_1_SEQUENCE=ACAAAGAAAGCAAAACCACCAC
PRIMER_LEFT_1=197,22
PRIMER_RIGHT_1=409,22
PRIMER_LEFT_1_TM=59.592
PRIMER_RIGHT_1_TM=60.424
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.8795
PRIMER_LEFT_2_PENALTY=0.846380
PRIMER_RIGHT_2_PENALTY=0.033137
PRIMER_LEFT_2_SEQUENCE=GGATCAAACATCATCAACCAAC
PRIMER_RIGHT_2_SEQUENCE=CTTCCTAGGTTCAACGACAACC
PRIMER_LEFT_2=308,22
PRIMER_RIGHT_2=518,22
PRIMER_LEFT_2_TM=59.154
PRIMER_RIGHT_2_TM=60.033
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.8795
PRIMER_LEFT_3_PENALTY=0.846380
PRIMER_RIGHT_3_PENALTY=0.033137
PRIMER_LEFT_3_SEQUENCE=GGATCAAACATCATCAACCAAC
PRIMER_RIGHT_3_SEQUENCE=TCCTAGGTTCAACGACAACCTT
PRIMER_LEFT_3=308,22
PRIMER_RIGHT_3=516,22
PRIMER_LEFT_3_TM=59.154
PRIMER_RIGHT_3_TM=60.033
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.9536
PRIMER_LEFT_4_PENALTY=0.529160
PRIMER_RIGHT_4_PENALTY=0.424392
PRIMER_LEFT_4_SEQUENCE=TAAGTCCACCTTCATCACCAAA
PRIMER_RIGHT_4_SEQUENCE=ACAAAGAAAGCAAAACCACCAC
PRIMER_LEFT_4=199,22
PRIMER_RIGHT_4=409,22
PRIMER_LEFT_4_TM=59.471
PRIMER_RIGHT_4_TM=60.424
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_01274 Ps_contig_mira-and-tgicl-ass_franssen_11872
SEQUENCE=ATCCTTTTCACATCAAAATGATCCTTCTTCTCACCTTCCCTAATATTTCCATATAAATACAATTCATGTAACTTTTCACATAATCATTTTGCTATTAACCAAAAACACAACAACCTTAGAATTACTATGGAAGTGATGATTCAAAGTTCAAAGATGGAGACATTTAATTTTAGTAATGGAACAATGAGTTCACCATATCTAAGTCCACCTTCATCACCAAAACGTTTTGGTGAATATTACTTAAGTGCCCCTTCAAGTCCTTCTAGGCTTTCTGAATTATATAGTCAAATTGATTATTTGTCCATAATGGATCAAACATCATCAACCAACAAAAACAACAACGTTGTTGTTGTTGATGATGATGATGTTTATGATCATGATGATGAAGGTGGTGGTTTTGCTTTCTTTGTTAATCACGATGAATCGAAGAATATGTCAACTCGTTCAGCTGAAGAACTTTTTCATGGTGGCAAAATTAAACCTTTTGAAGAAACTAAGGTTGTCGTTGAACCTAGGAAGCAACAAAACAACGTAGGAGTTGGTTTTGATGATGAAAGAAGAGGAAGAGAAAGAGAAAGAACAAAAGGAACAGATTCATCGTTGAATAATTCTGGTAGAAGAGTAACAAGATCACACTCTCCTTATAGAAAATCAAACTACACATTTGAGTTAGAAGAACAGAATTTCCAACAAAAACAACAACCTCGTATGATCAAAGAGGAATCGAAATCATCGAGTTCGAAGGGTTCGAGGAGATGGAAACTGAGTGATTTGTTGTTGTTTACGAAGTGCTTCGGAAGGGAGAGGATCAAACA
TARGET=355,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6923
PRIMER_LEFT_PENALTY=0.267873
PRIMER_RIGHT_PENALTY=0.424392
PRIMER_LEFT_SEQUENCE=AGTCCACCTTCATCACCAAAAC
PRIMER_RIGHT_SEQUENCE=ACAAAGAAAGCAAAACCACCAC
PRIMER_LEFT=201,22
PRIMER_RIGHT=409,22
PRIMER_LEFT_TM=60.268
PRIMER_RIGHT_TM=60.424
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.8323
PRIMER_LEFT_1_PENALTY=0.407941
PRIMER_RIGHT_1_PENALTY=0.424392
PRIMER_LEFT_1_SEQUENCE=TCTAAGTCCACCTTCATCACCA
PRIMER_RIGHT_1_SEQUENCE=ACAAAGAAAGCAAAACCACCAC
PRIMER_LEFT_1=197,22
PRIMER_RIGHT_1=409,22
PRIMER_LEFT_1_TM=59.592
PRIMER_RIGHT_1_TM=60.424
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.8795
PRIMER_LEFT_2_PENALTY=0.846380
PRIMER_RIGHT_2_PENALTY=0.033137
PRIMER_LEFT_2_SEQUENCE=GGATCAAACATCATCAACCAAC
PRIMER_RIGHT_2_SEQUENCE=CTTCCTAGGTTCAACGACAACC
PRIMER_LEFT_2=308,22
PRIMER_RIGHT_2=518,22
PRIMER_LEFT_2_TM=59.154
PRIMER_RIGHT_2_TM=60.033
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.8795
PRIMER_LEFT_3_PENALTY=0.846380
PRIMER_RIGHT_3_PENALTY=0.033137
PRIMER_LEFT_3_SEQUENCE=GGATCAAACATCATCAACCAAC
PRIMER_RIGHT_3_SEQUENCE=TCCTAGGTTCAACGACAACCTT
PRIMER_LEFT_3=308,22
PRIMER_RIGHT_3=516,22
PRIMER_LEFT_3_TM=59.154
PRIMER_RIGHT_3_TM=60.033
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.9536
PRIMER_LEFT_4_PENALTY=0.529160
PRIMER_RIGHT_4_PENALTY=0.424392
PRIMER_LEFT_4_SEQUENCE=TAAGTCCACCTTCATCACCAAA
PRIMER_RIGHT_4_SEQUENCE=ACAAAGAAAGCAAAACCACCAC
PRIMER_LEFT_4=199,22
PRIMER_RIGHT_4=409,22
PRIMER_LEFT_4_TM=59.471
PRIMER_RIGHT_4_TM=60.424
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_01275 Ps_contig_mira-and-tgicl-ass_franssen_11881
SEQUENCE=GGAAGARGCAGAAGAAAAGACCAATATACTTTTACTTGTTCTTTCCTTGATCCCATGAAGATTATTGAAAAATACTAAACTATTGTAAATGAGTCACAAACTCTTACTTCGATGTGCAAATCTAAGAAAAGCACCACCGTGGTGCAACCCACAAACAGAAACCCTAGGCGAACATCAACTTCTCCTTCGTCTTCGAATTTTCCATCTTCCAACAGTGAAGTTCCAATCACGGAATCAAGAAGTTACGTATACGGTGGAAAAGATAAAGAATGGTCCAAAACTTCTTCTTCTTCTGTATCTAGCCACGCTTCTCTTGCAAGCCTCAAACACACACTTCCAGAAAACCCTCAACAATATACCCTATCTCTGAGATCTCAACC
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01276 Ps_contig_mira-and-tgicl-ass_franssen_11955
SEQUENCE=ATKTCGATKGCACGCATTCTCTCCATCTCATCTCAAAATTCAAACGAAAAATCTTGAAGAAAACAACTTTGAATTTATGCTCTTACACCAACATCGAATTCATCACACCAAATCGAAATCTCCGCCAAAATCGAATCCTCCAAACACTCGTTCTTGCACGCGGATTAAGGAAACCAGAAGAAGAAGAACAACCTACCGGATTGGAGCTCCAGCGCAGTGGCTCTTCGGCGACAACATTGKTTTGGTATGGCAAANGGATTTAATACAAAGGATACCAAGGCTTGAAGATCAAGATAA
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01277 Ps_contig_mira-and-tgicl-ass_franssen_11968
SEQUENCE=CTCCAGTCTTCTGCATTCCCCTTCAAAAAACCTAMCTCTTGCTCTCAAGGTTTTTCTACATACATCTTCTTCCTCTCTCTTGATTTCACAGCAGCATCCCTGTTCCTCATCTGCCGTATTTGCTTCTTTGAGACGGGTTCATGAGGAACCTCTTCGGGAGTTAACGGATCAACTCTGTCTTTATCAGAAGGAGTCAAACTTTCCTCCGATTGAGGAAGCGGCTGAACGAGAATCTCCTCGAGAAGCTTATCATAGTCGGATTCCGGCGACGCGATTCCTTCTCCGTTGTCGAAATCTGTCATGAGCAAGTTCTCAAGCTGCGACATAGATGAATTAGGCGAGGGATGGTCTGCGAAGGAATCAGGAGGAATAATTCCATCTTGAAAAAAATCGGCGACTTCAAGCTCTGGTAAATCATCGAGGATAGATTCCCAATCTACAAYGTCCATGGAATGAGGAAGAAGATGGTGGTGGTGGTGGTGGTGGTKGTGGTGGTGGTGATTGAACGGGGTTAGTRAAGGTTATGCATAAAAAAGAAGAAGAAGAAAAAAAAAGCGGTTTATGAAGTTTGGGGTTTACACAGTTAGGATTTTATTACTTTTTGAGTCTTGTCTTGGTGATTC
TARGET=466,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01278 Ps_contig_mira-and-tgicl-ass_franssen_11968
SEQUENCE=CTCCAGTCTTCTGCATTCCCCTTCAAAAAACCTAMCTCTTGCTCTCAAGGTTTTTCTACATACATCTTCTTCCTCTCTCTTGATTTCACAGCAGCATCCCTGTTCCTCATCTGCCGTATTTGCTTCTTTGAGACGGGTTCATGAGGAACCTCTTCGGGAGTTAACGGATCAACTCTGTCTTTATCAGAAGGAGTCAAACTTTCCTCCGATTGAGGAAGCGGCTGAACGAGAATCTCCTCGAGAAGCTTATCATAGTCGGATTCCGGCGACGCGATTCCTTCTCCGTTGTCGAAATCTGTCATGAGCAAGTTCTCAAGCTGCGACATAGATGAATTAGGCGAGGGATGGTCTGCGAAGGAATCAGGAGGAATAATTCCATCTTGAAAAAAATCGGCGACTTCAAGCTCTGGTAAATCATCGAGGATAGATTCCCAATCTACAAYGTCCATGGAATGAGGAAGAAGATGGTGGTGGTGGTGGTGGTGGTKGTGGTGGTGGTGATTGAACGGGGTTAGTRAAGGTTATGCATAAAAAAGAAGAAGAAGAAAAAAAAAGCGGTTTATGAAGTTTGGGGTTTACACAGTTAGGATTTTATTACTTTTTGAGTCTTGTCTTGGTGATTC
TARGET=489,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01279 Ps_contig_mira-and-tgicl-ass_franssen_11968
SEQUENCE=CTCCAGTCTTCTGCATTCCCCTTCAAAAAACCTAMCTCTTGCTCTCAAGGTTTTTCTACATACATCTTCTTCCTCTCTCTTGATTTCACAGCAGCATCCCTGTTCCTCATCTGCCGTATTTGCTTCTTTGAGACGGGTTCATGAGGAACCTCTTCGGGAGTTAACGGATCAACTCTGTCTTTATCAGAAGGAGTCAAACTTTCCTCCGATTGAGGAAGCGGCTGAACGAGAATCTCCTCGAGAAGCTTATCATAGTCGGATTCCGGCGACGCGATTCCTTCTCCGTTGTCGAAATCTGTCATGAGCAAGTTCTCAAGCTGCGACATAGATGAATTAGGCGAGGGATGGTCTGCGAAGGAATCAGGAGGAATAATTCCATCTTGAAAAAAATCGGCGACTTCAAGCTCTGGTAAATCATCGAGGATAGATTCCCAATCTACAAYGTCCATGGAATGAGGAAGAAGATGGTGGTGGTGGTGGTGGTGGTKGTGGTGGTGGTGATTGAACGGGGTTAGTRAAGGTTATGCATAAAAAAGAAGAAGAAGAAAAAAAAAGCGGTTTATGAAGTTTGGGGTTTACACAGTTAGGATTTTATTACTTTTTGAGTCTTGTCTTGGTGATTC
TARGET=534,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01280 Ps_contig_mira-and-tgicl-ass_franssen_11982
SEQUENCE=CTTCGGTTGCTAAGTATTGTATAACAAGAAATATAAGGGAGTACAGCATGGGTTTATTGGGTTGAATGTTCTTACTATTGGTTTTGTTCCGCTGACAAATACTAGTGGAGATATTATAGCAACTCAAAGGGCTCAAGACTTTTGGATACGGTGGGTAATAAATCCATTAACATTGGGTGATTATCCTGATATAATGAAAAAGAATGTTGGCTCAAGGCTTCCTTCCTTCACTAAAGAGGAATCAAATCTGATCAGAAACTCAATTGACTTCTTAGGAATTTAACTTTTACAACACATTTTCGGTTAACAACTATCCCGAAAGCTTGGACATGGAGGATAGAACTTTCATGCAAGATATGGCAGCAGAAATGACAAYGCTTCCTAGAAAACGATACATTTANAGACGNGGTGCTAGNTTACCTTCAAGAATGATTATAGG
TARGET=218,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01281 Ps_contig_mira-and-tgicl-ass_franssen_11987
SEQUENCE=GACATTAATTTCTCTTCCTCTTKATACCAAAAAAAGCATATATTATAAACTATACTATATAAAAAAAATGGTTGATCCACGAAGATACCATTACCAACCCACAATTTCTTTTCCAGTTTCTGATAATATATCATCGCAACAAAAACCTCACACCAACAACAACAACAACAACATTTGTCAAAACAAAAAACATCAACAATGATCCACATGAATCTGGTATGTGTTCTCCTCCTTTATGGACAACAAAGAGTAAAAGCAACAACTACAGAAGTTCTTTCKCCTGAGTCAAAG
TARGET=154,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01282 Ps_contig_mira-and-tgicl-ass_franssen_11988
SEQUENCE=ACAACAAAGAGTAAAAGCAACAACTACAGAAGTCTTTCTCCTGAGTCAAAGACAGAAGCCATTGAAAGAGGTCAAAGAGAGTTAATGGAAATGGTCAAGAACATGCCAGAGTCAAGCTATGAACTCACTCTAAAAGATCTTGTAGAGGAACAACATCACCATCATCATCATCATCATCATGTTGAAAGAAAACAACAACAACAATATCAATAGGGTWGGTAAAGCAAGAAGTAGTTTGAAATAAACAAAAAGGCCATAGAAAAGAGAAGGGATGATGGAATAAGAGAAGTATTAGACCGG
TARGET=160,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01283 Ps_contig_mira-and-tgicl-ass_franssen_11988
SEQUENCE=ACAACAAAGAGTAAAAGCAACAACTACAGAAGTCTTTCTCCTGAGTCAAAGACAGAAGCCATTGAAAGAGGTCAAAGAGAGTTAATGGAAATGGTCAAGAACATGCCAGAGTCAAGCTATGAACTCACTCTAAAAGATCTTGTAGAGGAACAACATCACCATCATCATCATCATCATCATGTTGAAAGAAAACAACAACAACAATATCAATAGGGTWGGTAAAGCAAGAAGTAGTTTGAAATAAACAAAAAGGCCATAGAAAAGAGAAGGGATGATGGAATAAGAGAAGTATTAGACCGG
TARGET=191,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01284 Ps_contig_mira-and-tgicl-ass_franssen_11998
SEQUENCE=GGATACTACTTATGAACKAAATTGAAGAAGAAAAAAGAAAGAAAGAAGATAAGTAGAAAAACCAAAGCCTTAGAGAAAGAAGAAGAGAAGAGAGACAACGATGGGAACACCAGAGACTTCAAGAGAACCCTGCCCTGATCGAATTCTCGACGACATAGGCGGTGCCTTCGGTATGGGTGCCGTCGGTGGCTCCGCCTTCCACTTCCTCAAAGGACTCTACAATTCTCCCAAAGGCTCCCGCTTCGTCGGTGCTTCCCAAGCCGTTCGTCTCAACGCTCCCCGAGTCGGCGGTAGTTTCGCCGTTTGGGGTGGTCTCTTCTCCTCTTTCGACTGCACCATGGTCTACCTCCGCCAAAAAGAAGACCCTTGGAACTCTATCTTCGCCGGCGCCGCCACCGGTGGGTTTCTCTCAATGCGTCAAGGTTTGGTCGCTTCCGGTCGATCCGCTGCGTTTGGCGGTGTTCTCTTGGCGCTTATTGAGGGAGCGGGAATTATGCTTAACAAGGTTCTTAGCCCGCAGCAGCAGGTGCCTGCTGTCATGGATGAACCAATCCCTGGACATCCCAACGGCGGTTTTCCGGGAAAACAGGTTCCGCCGGCGGGTGACTCCGAAGCTAAACCGTGGTTCGGTGGTGGGTTGTTCGGTGAAGATAAGAAAAAGGATGAAGCTGTTTCTGATGGAGGAAGTGAGACCAAGATTTTGGAGAGTTTTGATGCTCCTCCTGTGCCAAATTTTGACTACAAATAATGTTTGGTTTTGGTTATAGTTTTTCTGAACCTTTTCTACTGTATTTTACTTATATATATGCAAGACATAGTTTTATCTTAATGTAAGAGAAAATTTACTAATTGGTCGGAAGATCTTATTTGTGTGCAGGGTTTGCAATGTTCCTCCCAATATAATTTCGGGAGAATTATTTTATGGGGTACACTGTTTTATCTAGAACAGGTCTTTTAGGTTTTGTAAAAATAGAGACATGTTTATGTTTCAGGTAGTATGAATGATTTTGTATTTAGCTCAAAGCTTTTAGTTATAGGCTGGAAATTTTGTGACTTGATTTTGATGATGTAATTTTAAGTTAATTTTTCATAG
TARGET=34,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01285 Ps_contig_mira-and-tgicl-ass_franssen_12022
SEQUENCE=ATGGTGGTGGTTATGAGAGAGGAAGAAGGATTTTATTTGTTTTATCTTTTTCAGAGAGAGAGAGATGAGATTTGTGAGGAATTGAAAGAGTGGAGGTAGCGTGAGGGAGTTTGAGAATTCTGTTAGTACCCCGA
TARGET=53,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4955
PRIMER_LEFT_PENALTY=0.246726
PRIMER_RIGHT_PENALTY=0.248820
PRIMER_LEFT_SEQUENCE=ATGGTGGTGGTTATGAGAGAGG
PRIMER_RIGHT_SEQUENCE=AGAATTCTCAAACTCCCTCACG
PRIMER_LEFT=0,22
PRIMER_RIGHT=120,22
PRIMER_LEFT_TM=60.247
PRIMER_RIGHT_TM=59.751
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.6102
PRIMER_LEFT_1_PENALTY=0.361394
PRIMER_RIGHT_1_PENALTY=0.248820
PRIMER_LEFT_1_SEQUENCE=GGTGGTGGTTATGAGAGAGGAA
PRIMER_RIGHT_1_SEQUENCE=AGAATTCTCAAACTCCCTCACG
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=120,22
PRIMER_LEFT_1_TM=60.361
PRIMER_RIGHT_1_TM=59.751
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=119
PRIMER_PAIR_PENALTY_2=1.0389
PRIMER_LEFT_2_PENALTY=0.246726
PRIMER_RIGHT_2_PENALTY=0.792166
PRIMER_LEFT_2_SEQUENCE=ATGGTGGTGGTTATGAGAGAGG
PRIMER_RIGHT_2_SEQUENCE=TTCTCAAACTCCCTCACGCTAC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=116,22
PRIMER_LEFT_2_TM=60.247
PRIMER_RIGHT_2_TM=60.792
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=1.1536
PRIMER_LEFT_3_PENALTY=0.361394
PRIMER_RIGHT_3_PENALTY=0.792166
PRIMER_LEFT_3_SEQUENCE=GGTGGTGGTTATGAGAGAGGAA
PRIMER_RIGHT_3_SEQUENCE=TTCTCAAACTCCCTCACGCTAC
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=116,22
PRIMER_LEFT_3_TM=60.361
PRIMER_RIGHT_3_TM=60.792
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=115
PRIMER_PAIR_PENALTY_4=1.2745
PRIMER_LEFT_4_PENALTY=1.025669
PRIMER_RIGHT_4_PENALTY=0.248820
PRIMER_LEFT_4_SEQUENCE=TGGTGGTGGTTATGAGAGAGG
PRIMER_RIGHT_4_SEQUENCE=AGAATTCTCAAACTCCCTCACG
PRIMER_LEFT_4=1,21
PRIMER_RIGHT_4=120,22
PRIMER_LEFT_4_TM=59.974
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_01286 Ps_contig_mira-and-tgicl-ass_franssen_12027
SEQUENCE=AGAAATGTTTAATTGTTTGGGCAGTGAAGAATTCAAACGAGTCTTCCACTTCTTTCGACAGTTGACATGGAAACAATCTGTCCACGCTTTTCTGATAAAGTGAATATCAATATACATATTTTCGTCACTCGAGAATCAGATCCTCCATTGGAAGAGGGATACGCTTTCAAACCGATAAAATCTTCACTCTGTCCCTTTCCAATGCCTAGTGATTACGGAATGTCTGGTTTGGTTGGTACAGGAAACAATGTTTGGTCAGGACTATATGTCATTTCATCTACCTTGGGCTTTGTGATCTTATTTGCTTTGTTGAATATTTACTATATAAATCCATTTGGCGTGTATAAATGGTGGTACAAAGGGTTACTATTTGTAGTCTGCATGATTGCAAGTGTCGTTATCTTTGGTGGCATAGTGGTTGGATTTTGGCATATATGGGAAAAGCAAAGTTCTCTCAAAGATAATTCCAATAACATCAAGGTTGATAAAATCGAGCAAAACGGCTCCGCAGCTTCCAAGGATCCAAGTCCAGACAATCTTGCAAAGTTAACCGACGTTCGATATGGTTCTAGGCCAGACTTTAAAGAGATTTTTGAATTGATGTCAGAGAAATGGGGCCATGCTGATGTTGGTGTTATAGTTTGTGGTCCTCTAACTCTTCAAGCAAGTGTTGCACAAGAAATCAGGTCACATAGTTTGACAAGACAAACTTATCATCCCATCTTCCATTTCCACAGTCATAGCTTTGATCTTTAGCTTCCTCTCTATGATTGAAAGATGGAAGATGTGCCTTCAATAATGGAAAAACATGCATATATATTGTATATATATAGATATAGCAGATTCTCTGGTTGAGACTAGTCATGGCGTAGTTAGTGAATACTTTGCACCTTTWAWANATGGCAAAAGGAAACTATATTAGTGCCAAATTG
TARGET=823,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01287 Ps_contig_mira-and-tgicl-ass_franssen_12031
SEQUENCE=TCGGGGATCATAAACTAAAATTAGTCTCCAATCTTCAATAAGCTCTCTCTCTAAGCTATTATTTTGTGTTTGCATATTGAAAAATGGCATGCATTAGTAGCTGCAACATGAACAAGGCAATAGTGTTGATGGTGTTTCTTGTGGGACTTGTGTTGGCTGCTGAGGCGAGGACACAAGATGACGAGTTCGCTCCGGCGCCGGCACCGTCAAAGGATGCCGGAGCTTGGGTTTTTGGTGACTTATTCTGGAG
TARGET=43,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01288 Ps_contig_mira-and-tgicl-ass_franssen_12046
SEQUENCE=TGTCTTGCTAGCATTTCATCAGAATGCTCATTTTGACGCACTATATCCAACCTATTGCTATTAATAAACGCATGCGGTCTACTCTCCACTTCGGAAGGTCTGACCACCTCAACATCTGTCTGATAATTGACTGACTGCCATCTACCAGAAGCCACCTTATCAAGGGCACTAGCATGAGTCAACTTGGAAACAGCATTAGCAGCAGACCAAGCAGCTTGTTCCGACTCAACAGTACCTGCCACCTCCTTCCTCGTTGACCACGCATTTGGATAACCACCACCACCACCAGAGCCAGCAGCAGCTGATCCATGCTCCTTACTAGCTCCAAAACTCTGAGAATTTACTCCCACATGAACTGTCTCGGCAAGCCTAGCCGAGTACGAATTAGCATTCCCAACAGGGGACACCGGAGCCACTTGCCTGCTTAACGAACTGCTTCTCTTCACCTCAACACGAGAAGGCAGAGGAGCACGGAGGCTCTCATCACTAATTTGTCTCCTTGGGGCAGAACTTCCATCCAGCGGCTTCCTCTCATCCTCATCAAAATTCCTCCCAATTGTGGTAGTATGTGGAAGAAACAGGGATTTGTCATCGTAATGCCTAGTTGCAGGCGAAGTGTGTGGCCGAGACCAATGATCCGGCCTCCCCATCGGGTTTCCCCACGACGTAGGCCGGTCATAACCAACACGGTCGGAAGGCCTCACAGTTACGCCGGGAGCCGAAGGGAGAGGGAGATCAGAAGGTATAGAACCGCCATGGAAGTCCTTGAGCGTCATGGTGCTCCCACTACCTTTCTTCTTCGACATTATCCCCTTCACCTTCTCCTTCTTCCTCGCAAAATCAGAAAGAAAAAAAACAGAAAAAAAWTWCAAAAAATAAATCGTAACCCCGGAAGCTAAATTTGAGGATGGTTTTTGTTGTTGATTAGAGGAAAGTGAGAAAAGAAACCCACGAATGGGTGAAAGAGAGAAACGGAGAAGGAACAGAATTAGTGAAATTTGGGTGCGAAAAAAGAAAAGGGAAAAAAAAGTGAAGAGA
TARGET=273,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01289 Ps_contig_mira-and-tgicl-ass_franssen_12057
SEQUENCE=ATCTCTTCAACAACAATCTCAGCATCACAGTAATCATAATCAACAACATCACTCAATAGCTTCTCAAGGCTTCCACTGAGCTTATTCAAACTCACAATTTCAATATTTGCCACATGTTCATTGTGAGTATTACTTGAACCATTTGATAAGTGTGATGATACAAAACTCAATGATGATGATGCTTCATTTGAATTATCCATGACCCTTTTAAGCCTCCAATTGAGTCAACAGATTGGTTGACACAAGAATGCTTTCTAAGTCCATTCCAA
TARGET=170,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1674
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=0.154023
PRIMER_LEFT_SEQUENCE=TCAATAGCTTCTCAAGGCTTCC
PRIMER_RIGHT_SEQUENCE=GAAAGCATTCTTGTGTCAACCA
PRIMER_LEFT=52,22
PRIMER_RIGHT=254,22
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=60.154
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=0.2669
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=0.253482
PRIMER_LEFT_1_SEQUENCE=TCAATAGCTTCTCAAGGCTTCC
PRIMER_RIGHT_1_SEQUENCE=TGTTGACTCAATTGGAGGCTTA
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=229,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=59.747
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.3901
PRIMER_LEFT_2_PENALTY=0.236064
PRIMER_RIGHT_2_PENALTY=0.154023
PRIMER_LEFT_2_SEQUENCE=CAACAACAATCTCAGCATCACA
PRIMER_RIGHT_2_SEQUENCE=GAAAGCATTCTTGTGTCAACCA
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=254,22
PRIMER_LEFT_2_TM=59.764
PRIMER_RIGHT_2_TM=60.154
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=0.4351
PRIMER_LEFT_3_PENALTY=0.236064
PRIMER_RIGHT_3_PENALTY=0.199067
PRIMER_LEFT_3_SEQUENCE=CAACAACAATCTCAGCATCACA
PRIMER_RIGHT_3_SEQUENCE=ATTGGAGGCTTAAAAGGGTCAT
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=219,22
PRIMER_LEFT_3_TM=59.764
PRIMER_RIGHT_3_TM=60.199
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.5466
PRIMER_LEFT_4_PENALTY=0.392624
PRIMER_RIGHT_4_PENALTY=0.154023
PRIMER_LEFT_4_SEQUENCE=AGGCTTCCACTGAGCTTATTCA
PRIMER_RIGHT_4_SEQUENCE=GAAAGCATTCTTGTGTCAACCA
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=254,22
PRIMER_LEFT_4_TM=60.393
PRIMER_RIGHT_4_TM=60.154
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_01290 Ps_contig_mira-and-tgicl-ass_franssen_12067
SEQUENCE=TTTCCCGGCCCTGTCCTTGAAGGTTTAGAAGATGTGCAGGAGGAGGAGGATGGGTTGGCATGCATGGTCTGACCGGGAAAGGATACAGTTTAAGACCTACTGATTTACTGGGTG
TARGET=38,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01291 Ps_contig_mira-and-tgicl-ass_franssen_12083
SEQUENCE=AATCGGAACCGTTGGTTGACGTCTTGGAGATGATTAAAAGACATGAAAATTTTTCATTATTTGAGCGCCGTCTCGAGAAAAATCAGGATTTGGATAGATACAAAGACATTGTGAGACAGCACCTGGACTTGGAAACCATACATTCGAGACTCCAAAAGGGTCACTATTCCTCATGCACTAACACATTYTTCCGTGACCTCCTCCTCCTCTTCACCAACGCCACTGTTTTCTTCCATCATGACTCAACCGAATCACGCGCGGCGCAGCAGCTGATTCGCCTTGTCATTGCTGAGATGAGAAGCTGCAGTCGAACACAGT
TARGET=197,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01292 Ps_contig_mira-and-tgicl-ass_franssen_12096
SEQUENCE=ATGGAGATGGATATGGACATGGATGCTATCCACCACCYCGACTTCTCCGGACACAGCAGTACCCTCACTAACACCCCTACTTCCGACGATGGTTATGGTTGCAACTGGAACCACTGGTCCCCCATCGTCAACTGGGACACCTTCACAGGTGCCCCGGATGATTTTCACCACCTAATGGATACCATCATCGACGACAGAACCACCGTCCTTGACCAGCTCAGTCCAACTACAACTACAACCACAACCACAACCACCGAGGAGGAAGAAAGAAAACAAGAAAACAAGAAAACAAACTACCACCACCACCACAACAGAAACAGTTGGCGTTGGCGATGGCGATGATTTGAAGGGGCTAAAACTAGTGCACCTATTAATGGCTGGCGCAGAAGCCTTAACCGGCTCCACCAAGAGCCGCGACTTAGCTCGAGTGATATTGGTTCGGCTCAAGGAGTTGGTTTCACAACACGCCAACGGCTCCAACATGGAAAGACTCGCCGCTTATTTCACCGAAGCTCTTCAGGGCCTACTAGAAGGCGCTGGGGGTGCGCACAGCAACAACAACAAACATTACCTCACCACTAACGGGCCCCACGACAACCAAAACGACACACTCGCTGCTTTCCAACTACTTCAAGACATGTCTCCTTATGTCAAGTTCGGCCACTTCACCGCCAATCAAGCCATACTCGAATCCGTGGCCCACGAACGCCGAGTCCATGTCATTGACTACGACATCATGGAAGRGGTCCAATGGGCTTCACTAATCCAAGCCCTAGCTTCCAGCAACAACAGCCCGCATCTCCGGATCACGGCATTATCGCGAACCGGAACCGGACGGAGATCAATCGCCACCGTACAAGAAACCGGCCGCCGTTTAACCTCTTTTGCCGCTTCCCTCGGCCAGCCGTTTTCTTTTCACCACTGCAGGTTAGACTCCGACGAAACTTTCCGTCCTTCTTCTTTGAAGCTTGTTCGAGGAGAGGCTTTGGTTTTCAACTGCATGCTGAATCTGCCTCATCTTAGTTACCGTGCACCTGATTCGGTTGCTTCGTTTTTAAACGGAGCAAAAGCTTTAAACCCGAAGCTTGTGACGTTGGTGGAAGAGGAAAATGGATCTGTTGTTGGTGGATTCGTGGAGCGGTTCATGGACTCGCTTCACCATTACTCAGCGGTGTTTGATTCGTTAGAGGCTGGGTTTCCTRTGCAGAATCGGGCCAGGGCTTTGGTGGAGCGAGTATTTTTCGGGCCCCGAATAGCGGGCTCGTTGGGCCGGATATATAGAACAGGTGGAGACGGAGAGGAGGAGAGAAGGTCGTGGGGGGAGTGGTTAGGTGCGGCGGGGTTTAGAGGAGTTCCGGTAAGCTTTGCAAATCACTGCCAAGCGAAGCTGTTATTGGGTCTTTTCAATGACGGGTATAGGGTCGAAGAGGTGGGTTTGGGTAGTAATAAGTTGGTGTTGGATTGGAAATCAAGGCGTTTGCTTTCTGCTTCCGTTTGGACTTGTTCTTCGGATTCTGATTTATAG
TARGET=296,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01293 Ps_contig_mira-and-tgicl-ass_franssen_12096
SEQUENCE=ATGGAGATGGATATGGACATGGATGCTATCCACCACCYCGACTTCTCCGGACACAGCAGTACCCTCACTAACACCCCTACTTCCGACGATGGTTATGGTTGCAACTGGAACCACTGGTCCCCCATCGTCAACTGGGACACCTTCACAGGTGCCCCGGATGATTTTCACCACCTAATGGATACCATCATCGACGACAGAACCACCGTCCTTGACCAGCTCAGTCCAACTACAACTACAACCACAACCACAACCACCGAGGAGGAAGAAAGAAAACAAGAAAACAAGAAAACAAACTACCACCACCACCACAACAGAAACAGTTGGCGTTGGCGATGGCGATGATTTGAAGGGGCTAAAACTAGTGCACCTATTAATGGCTGGCGCAGAAGCCTTAACCGGCTCCACCAAGAGCCGCGACTTAGCTCGAGTGATATTGGTTCGGCTCAAGGAGTTGGTTTCACAACACGCCAACGGCTCCAACATGGAAAGACTCGCCGCTTATTTCACCGAAGCTCTTCAGGGCCTACTAGAAGGCGCTGGGGGTGCGCACAGCAACAACAACAAACATTACCTCACCACTAACGGGCCCCACGACAACCAAAACGACACACTCGCTGCTTTCCAACTACTTCAAGACATGTCTCCTTATGTCAAGTTCGGCCACTTCACCGCCAATCAAGCCATACTCGAATCCGTGGCCCACGAACGCCGAGTCCATGTCATTGACTACGACATCATGGAAGRGGTCCAATGGGCTTCACTAATCCAAGCCCTAGCTTCCAGCAACAACAGCCCGCATCTCCGGATCACGGCATTATCGCGAACCGGAACCGGACGGAGATCAATCGCCACCGTACAAGAAACCGGCCGCCGTTTAACCTCTTTTGCCGCTTCCCTCGGCCAGCCGTTTTCTTTTCACCACTGCAGGTTAGACTCCGACGAAACTTTCCGTCCTTCTTCTTTGAAGCTTGTTCGAGGAGAGGCTTTGGTTTTCAACTGCATGCTGAATCTGCCTCATCTTAGTTACCGTGCACCTGATTCGGTTGCTTCGTTTTTAAACGGAGCAAAAGCTTTAAACCCGAAGCTTGTGACGTTGGTGGAAGAGGAAAATGGATCTGTTGTTGGTGGATTCGTGGAGCGGTTCATGGACTCGCTTCACCATTACTCAGCGGTGTTTGATTCGTTAGAGGCTGGGTTTCCTRTGCAGAATCGGGCCAGGGCTTTGGTGGAGCGAGTATTTTTCGGGCCCCGAATAGCGGGCTCGTTGGGCCGGATATATAGAACAGGTGGAGACGGAGAGGAGGAGAGAAGGTCGTGGGGGGAGTGGTTAGGTGCGGCGGGGTTTAGAGGAGTTCCGGTAAGCTTTGCAAATCACTGCCAAGCGAAGCTGTTATTGGGTCTTTTCAATGACGGGTATAGGGTCGAAGAGGTGGGTTTGGGTAGTAATAAGTTGGTGTTGGATTGGAAATCAAGGCGTTTGCTTTCTGCTTCCGTTTGGACTTGTTCTTCGGATTCTGATTTATAG
TARGET=553,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01294 Ps_contig_mira-and-tgicl-ass_franssen_12097
SEQUENCE=ATGGAGATGGATATGGACATGGATGCTATCCACCACCTCGACTTCTCCGGACACAGCAGTACCCTCACTAACACCCCTACTTCCGATGATGGTTATGGTTGCAACTGGAACCACTGGTCCCCCATCGTCAACTGGGACACCTTCACAGGTGCCCCGGATGATTTTCACCACCTAATGGATACCATCATCGACGACAGAACCACCGTCCTTGACCAACTCAGTCCAACTACAACTACGACCACAACCACAACCACCGAGGAGGAAGAAGAAACAGAAACAACTACCACCACCACAACAACAGAAACAGTTGGCGTTGGCGATGGCGATGATTTGAAGGGGCTAAAACTAGTGCACCTATTAATGGCTGGCGCAGAAGCCTTAACCGGCTCCACCAAGAGCCGCGACTTAGCTCGAGTGATATTGGTTCGGCTCAAGGAGTTGGTTTCACAACACGCCAACGGCTCCAACATGGAAAGACTCGCCGCTTATTTCACCGAAGCTCTTCAGGGCCTACTAGAAGGCGCTGGGGGTGCGCACAGCAACAACAACAAACATTACCTCACCACTAACGGGCCCCACGACAACCAAAACGACACACTCGCTGCTTTCYAACTACTTCAAGACATGTCTCCTTATGTCAAGTTCGGCCACTTCACCGCCAATCAGGCCATACTCGAATCCGTGGCCCACGAACGCCGCGTCCATGTCATTGACTACGACATCATGGAAGGGGTCCAATGGGCTTCACTAATCCAAGCCCTAGCTTCCAGCAACAACAGCCCGCATCTCCGGATCACGGCATTATCGCGAACCGGAACCGGACGGAGATCAATCGCCACCGTACAAGAAACCGGCCGCCGTTTAACCTCTTTTGCCGCTTCCCTCGGCCAGCCGTTTTCTTTTCACCACTGCAGGTTAGACTCCGACGAAACTTTCCGTCCTTCTTCTTTGAAGCTTGTTCGAGGAGAGGCTTTGGTTTTCAACTGCATGCTGAATCTGCCTCATCTTAGTTACCGTGCACCTGATTCGGTTGCTTCGTTTTTAAACGGAGCAAAAGCTTTAAACCCGAAGCTTGTGACGATGGTGGAGGAGGAAAATGGATCTGTTGTCGGTGGATTCGTGGAGCGGTTCATGGACTCGCTTCACCATTACTCAGCGGTGTTTGATTCGTTAGAGGCTGGGTTTCCTATGCAGAATCGGGCCAGGGCTTTGGTGGAGCGAGTATTTTTCGGGCCCCGAATAGCGGGCTCGTTGGGCCGGATATATAGAACAGGTGGAGACGGAGAGGAGGAGAGAAGGTCGTGGGGGGAGTGGTTAGGTGCGGCGGGGTTTAGAGGAGTTCCGGTAAGCTTTGCAAATCACTGCCAAGCGAAGCTGTTATTGGGTCTTTTCAATGACGGGTATAGGGTCGAAGAGGTGGGTTTGGGTAGTAATAAGTTGGTGTTGGATTGGAAATCAAGGCGTTTGCTTTCTGCTTCCGTTTGGACTTGTTCTTCGGATTCTGATTTATAG
TARGET=540,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01295 Ps_contig_mira-and-tgicl-ass_franssen_12189
SEQUENCE=ATAAACAAACAACTGTAACAAAACAGTGGAAAGTGTACGATGTAAAGGAACATCCTAGGAAACAGACAATGAACAACAACAACAAGGTGTTACTACATGTAAACGACGTCGTTCGTAGTGGTGATAATCAGTTAGCTTCAAAACAAGATCCAAAACCAGTGGATGAAGATCTATATAAGATCYCTCCTGAGCTTCTTCGTACAACAAAAACGGAAAAAAATGCTG
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01296 Ps_contig_mira-and-tgicl-ass_franssen_12194
SEQUENCE=CTGCATTTATAAAGTTACGTATAAATGCAGAATGTCCTAACTATATATATTTCAATACACACTCTCGGTTAATTGAAATTTATATAAATCCAAATTTATATAAAGTTCATTATGTAGAAAAAAGTGTGATAAAAAAATGCGTGTTAAAAGTTTGTTTTGAAGGATGGTATTTTGTGATGGTTGAAACATCCAAACTATCCTATGATTGTAATGTTTTTGTTTKTTTTTGTGTATTGGTGCTGTGCTATTGTTTGGTTTCAATATATGTATTCTTGGTGAAATAATTTGATTTGGATGATATCCTAATCTTAAATGTATAAATATATACGTGTGCGGGTGTGAAAAAGAAAGAAAGGA
TARGET=344,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01297 Ps_contig_mira-and-tgicl-ass_franssen_12207
SEQUENCE=GCTGCGGCTATATACKGATCCATTGCACAGTCTACAGTCGACACATTTGTCTCTCTTTTCTCTCTCTCGGTTTGTGTGCTGTTTTATGTGTACATGTCCATGACACCAACTTATCCTTATTCACTATCTTCTCCTCCTATGCCTTTTGATCTTAATAACAATGAAGATCACAATCATCATCTCTTCTATACAAATATTCATCAACCCTCTTCTTCATCTTCTTCTCTATCATATTCCACACTTTTCAACCAAAATCAAAATCAAGGAGACTCTTATTCTTATGAACCAAATCACTTACAAAGTGATGTACAGGTTATGAAGTTTGTTCCTTCTAGTGGATCACGTGATCATCAAGAAGTAAAGAAAGAAAATGGAGAATGACAGAATTTGAAGGCCGAGGATAGTAG
TARGET=59,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01298 Ps_contig_mira-and-tgicl-ass_franssen_12231
SEQUENCE=ACGACTTCGCCGGGGAAAAGGTAAAGTACCGGGTATCAAACCACTGACTTACCAAGTCTTCAAAAGGAAAGCGATCGTGCTGAGGAAACAGACAGCTCCGCTGGCACACTGCATGGGGAAAAGAGGTAATAACTCCACGTGCAACCCTGGAGGGGATGTTAATAACAGCTCTACTCGCGACTCCGAGGGGAGTATCAATCAATCAGTTATACTCATGRYTGTGCTGAGGAGACAAATAAAGTCTGGGGCATACAACCCACTGGAGAAAGTGACGGGGCACCAAGATGACAGACCATCAATCACCGAAACTTCTATAAGGAAACACCCATGCTGGGAAAACTGCTGCTGGAGAGAACGAAGTCTTCAACAACACT
TARGET=194,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01299 Ps_contig_mira-and-tgicl-ass_franssen_12234
SEQUENCE=TTTCTGGCACAACCACTTCTCAAACGGTAACCACGGCTTGAGACTAGCTTATGCTTGCAATGATGCATGATTGATTTTTTCTGCGTAATGCTCCATAAACATGGAAATGCTACGCGATTATTTATTCTATGCAATATGCTATGCTTATTTACATGATGAATGYATAAAAAGTATCCCCCTCAAGGGACTCTTCTGGGGAATACGAGGCACTCTGCTGAGGAACTCGTCACTGCTCCGATTCCACCCTGCTGGGGAATAGCACTGCTGCTAGGAAAAAGGCAACCCTTGCGTAGGGGAAAAACCTCCTTTGCACCCAATCCGCTCGGGAATTGCGTGGGGATAAGCCACCACCACCACTTCG
TARGET=345,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01300 Ps_contig_mira-and-tgicl-ass_franssen_12260
SEQUENCE=TTGGCGTGTTGTAGCAGGTGTTGTGCGTTTTGGAATGCTGTAGTTTAGGACTAGCGTTTCAAGTTGTGCCCATGGCTTGGCGCTGGCTTTGGTGAAGCTTACGCGTATCCGTTGTTTTGGAGCTTTGGATGCATCTTTTGAAGCTTACGCTCAGGCTTTTCACTTGCAGAACTATTATTCTCACTTGCTTTTTCCTCCATTGCATTACTTATTATTATTATTTATTTTTATGGACAAACTACATGCATATGGTTTTGGATCATGTTATGGAATTATTTGGAAAATGGTTTATTGGTTGTTAAGGGGATATGGAAATTGTTTATGAATAAAAAATGGTTTATGAAAYTATTATCAAATGAATTTGG
TARGET=209,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01301 Ps_contig_mira-and-tgicl-ass_franssen_12261
SEQUENCE=GCAGCCAAACACAGCATTCCAAACGCACAACACCTGCTACAACACGCCAAGCCAAAACGCGATGCAATTCCAACTACAAGCCAAGCTACTTCCAAGCACCAAATAAGCTACAGTTACAATTAAGCCGGCAAACCGCAGCTAAACGCGAGCCAGCAAAAGCCAATTCCACACCTCTCTCAAAGCCTCTTCGATTCATCCTCTTCTTCTCAACCTCATTCTTCTTCTTCTCCTNTATCAATACTTCAATCC
TARGET=216,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01302 Ps_contig_mira-and-tgicl-ass_franssen_12292
SEQUENCE=CGTTCATTCTAATTGCAGATTTTGTCATTTTTCAAGCAATGCATGTAATGTGGAAAGATTATATAGCACTACTGCTTAAAACCACCGGGAGAAATCAATTGGCTCAATGTCTCCTTGGCGCAGATCTACATGGTGCTATTATTTTAAGTTCGAGTACACTCTATTTTTGTTTTTACTTTTGGTTGAGTTGTGAACACCTACTATAGAAAATGCTAAACCATTTGTCCTTTATACGGTTGTGATATGTAATTATTTCACATGCATTGAAAGAAAGAAAGTTGTATTGCAAGT
TARGET=266,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01303 Ps_contig_mira-and-tgicl-ass_franssen_12295
SEQUENCE=CGGACATCGTTTCCTTTCCTTTTCTATCTGAAACGGAAGATACACAAATGGATAATCTATATCCTTACGTTCATCTTCTCCCTAACGGTAAACTGTTTGTGTTTGCGAATACGAAATCGGTTATGTACGATTTCGTTGAGAATCGTGTTGTTAAGGTTTACCCGGAGTTAGACGGTGGTCCGAGGAATTATCCGTCGGCGGGTTCTTCGGTGATGTTAGCTTTGGAAGGTGAATTTTCTGAGGCGGTTGTTGTTGTTTGCGGCGGTGCTCAATACGGTGCGTTTTTAGAACGGAATGTAGATACGCCGGCGCATGGAAGCTGCGGCCGGATAATGGCGACGGGAGAAAACCCGGTTTGGGAAATGGAGGATATGCCGTTCCAGAGAATAATGGGTGATATGGTAATTTTACCAAACGGTGACGTTTTGATTATAAATGGTGCTATGAAAGGGACGCAAGGTTTTGAATTGGGTTCTGATCCGTGTTTGAACCCGGTTTTATATCGGCCCGATGAACCAGCCGGGTTACGGTTCATTGGTTTTGAATC
TARGET=246,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4213
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.312329
PRIMER_LEFT_SEQUENCE=GCTTTGGAAGGTGAATTTTCTG
PRIMER_RIGHT_SEQUENCE=GGATCAGAACCCAATTCAAAAC
PRIMER_LEFT=218,22
PRIMER_RIGHT=480,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=59.688
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.4213
PRIMER_LEFT_1_PENALTY=0.108929
PRIMER_RIGHT_1_PENALTY=0.312329
PRIMER_LEFT_1_SEQUENCE=GCTTTGGAAGGTGAATTTTCTG
PRIMER_RIGHT_1_SEQUENCE=GATCAGAACCCAATTCAAAACC
PRIMER_LEFT_1=218,22
PRIMER_RIGHT_1=479,22
PRIMER_LEFT_1_TM=60.109
PRIMER_RIGHT_1_TM=59.688
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.5658
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.312329
PRIMER_LEFT_2_SEQUENCE=TGATGTTAGCTTTGGAAGGTGA
PRIMER_RIGHT_2_SEQUENCE=GGATCAGAACCCAATTCAAAAC
PRIMER_LEFT_2=210,22
PRIMER_RIGHT_2=480,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=59.688
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=0.5658
PRIMER_LEFT_3_PENALTY=0.253482
PRIMER_RIGHT_3_PENALTY=0.312329
PRIMER_LEFT_3_SEQUENCE=TGATGTTAGCTTTGGAAGGTGA
PRIMER_RIGHT_3_SEQUENCE=GATCAGAACCCAATTCAAAACC
PRIMER_LEFT_3=210,22
PRIMER_RIGHT_3=479,22
PRIMER_LEFT_3_TM=59.747
PRIMER_RIGHT_3_TM=59.688
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.6542
PRIMER_LEFT_4_PENALTY=0.341875
PRIMER_RIGHT_4_PENALTY=0.312329
PRIMER_LEFT_4_SEQUENCE=GGTTCTTCGGTGATGTTAGCTT
PRIMER_RIGHT_4_SEQUENCE=GATCAGAACCCAATTCAAAACC
PRIMER_LEFT_4=200,22
PRIMER_RIGHT_4=479,22
PRIMER_LEFT_4_TM=59.658
PRIMER_RIGHT_4_TM=59.688
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_01304 Ps_contig_mira-and-tgicl-ass_franssen_12318
SEQUENCE=AGCATTGCACACCGCTATTCGATGCATCAGTGATCATAAGAAGTACTATGAAAAGGTTTTGCGTAGCGCGTTGAAAAGGATTGGAACAGACGAAGATGGACTGACTCGTGTTGTGGTAACGCGAGCTGAGAAGGTATTTATTTATTTACCAAATGTATTACGATGACTTGCCTTAGTTTGGGGTATTTTAGTTCGGGTTACCGTTAGAAACGAAAAGGAAAGGAAAAAGTCGACCCTAGCGAAGTTAATTAAGTTTATTTTTCTTTAANAAG
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0883
PRIMER_LEFT_PENALTY=0.073355
PRIMER_RIGHT_PENALTY=0.014945
PRIMER_LEFT_SEQUENCE=GGACTGACTCGTGTTGTGGTAA
PRIMER_RIGHT_SEQUENCE=GGTCGACTTTTTCCTTTCCTTT
PRIMER_LEFT=97,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=60.073
PRIMER_RIGHT_TM=59.985
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=138
PRIMER_PAIR_PENALTY_1=0.1478
PRIMER_LEFT_1_PENALTY=0.132841
PRIMER_RIGHT_1_PENALTY=0.014945
PRIMER_LEFT_1_SEQUENCE=ACTGACTCGTGTTGTGGTAACG
PRIMER_RIGHT_1_SEQUENCE=GGTCGACTTTTTCCTTTCCTTT
PRIMER_LEFT_1=99,22
PRIMER_RIGHT_1=234,22
PRIMER_LEFT_1_TM=60.133
PRIMER_RIGHT_1_TM=59.985
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.2008
PRIMER_LEFT_2_PENALTY=0.073355
PRIMER_RIGHT_2_PENALTY=0.127478
PRIMER_LEFT_2_SEQUENCE=GGACTGACTCGTGTTGTGGTAA
PRIMER_RIGHT_2_SEQUENCE=GCTAGGGTCGACTTTTTCCTTT
PRIMER_LEFT_2=97,22
PRIMER_RIGHT_2=239,22
PRIMER_LEFT_2_TM=60.073
PRIMER_RIGHT_2_TM=60.127
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=143
PRIMER_PAIR_PENALTY_3=0.2348
PRIMER_LEFT_3_PENALTY=0.219854
PRIMER_RIGHT_3_PENALTY=0.014945
PRIMER_LEFT_3_SEQUENCE=AGATGGACTGACTCGTGTTGTG
PRIMER_RIGHT_3_SEQUENCE=GGTCGACTTTTTCCTTTCCTTT
PRIMER_LEFT_3=93,22
PRIMER_RIGHT_3=234,22
PRIMER_LEFT_3_TM=60.220
PRIMER_RIGHT_3_TM=59.985
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=142
PRIMER_PAIR_PENALTY_4=0.2370
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.127478
PRIMER_LEFT_4_SEQUENCE=AAAGGATTGGAACAGACGAAGA
PRIMER_RIGHT_4_SEQUENCE=GCTAGGGTCGACTTTTTCCTTT
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=239,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=60.127
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_01305 Ps_contig_mira-and-tgicl-ass_franssen_12323
SEQUENCE=TTACAAAGCTTGTTTGTTTGTTCCTGCTGTAGTATAACCCAGAGTTATTTCCGTGGCTAATTTACCAAATGAAACAACCAAATATAGTCTTATATATATTCGACTCTGGAAAAGTTTTTCTGAAAGGAACCAAGTCGAGAAATGAGATTTACACAGCCTTTGAGAACATTTATCCAGTGCTTACTGAGTTCAGAAAAAACCAACAATGATCTTCGTCATGTGCAAGAGCTAAGGTTTTCCGGCTATGAAGGTTGTTCTTTTGCAAGGGCTCAATACAAAGTTAGTTAAAATTTAAAA
TARGET=10,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01306 Ps_contig_mira-and-tgicl-ass_franssen_12329
SEQUENCE=CTCTGATTTTTGAACGGTAGTGTCGGAGGATTTTGATTTGTTGAAACCAGACATGAAAACACGGAGCTTGGTGGCGGATTTCAAGAACGAGGCGGTGAACTGAGGTTTGGAGTTAGGTTCGGAGGAGTTCAGTTCCGACTGAACTTGGTGGTTGGTTAGAGGAGAAAGCGTAAAGCCGAGTTCAGTCTCAGACTCGTTTGGTTCTTCTTCTTCAGGTACAGTGAACTCTATGTCAAAGAAAGGCTCGTCTTCTTCTTCTCCTTCTTCGTCGGTAGTGGTTTCGACGTAG
TARGET=202,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0445
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TGATTTTTGAACGGTAGTGTCG
PRIMER_RIGHT_SEQUENCE=CTACCGACGAAGAAGGAGAAGA
PRIMER_LEFT=3,22
PRIMER_RIGHT=274,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.1115
PRIMER_LEFT_1_PENALTY=0.099308
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=AACTTGGTGGTTGGTTAGAGGA
PRIMER_RIGHT_1_SEQUENCE=CTACCGACGAAGAAGGAGAAGA
PRIMER_LEFT_1=141,22
PRIMER_RIGHT_1=274,22
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=0.1115
PRIMER_LEFT_2_PENALTY=0.099308
PRIMER_RIGHT_2_PENALTY=0.012153
PRIMER_LEFT_2_SEQUENCE=GAACTTGGTGGTTGGTTAGAGG
PRIMER_RIGHT_2_SEQUENCE=CTACCGACGAAGAAGGAGAAGA
PRIMER_LEFT_2=140,22
PRIMER_RIGHT_2=274,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=135
PRIMER_PAIR_PENALTY_3=0.1556
PRIMER_LEFT_3_PENALTY=0.032347
PRIMER_RIGHT_3_PENALTY=0.123278
PRIMER_LEFT_3_SEQUENCE=TGATTTTTGAACGGTAGTGTCG
PRIMER_RIGHT_3_SEQUENCE=CGACGAAGAAGGAGAAGAAGAA
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=270,22
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=60.123
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.1696
PRIMER_LEFT_4_PENALTY=0.157415
PRIMER_RIGHT_4_PENALTY=0.012153
PRIMER_LEFT_4_SEQUENCE=CTGAGGTTTGGAGTTAGGTTCG
PRIMER_RIGHT_4_SEQUENCE=CTACCGACGAAGAAGGAGAAGA
PRIMER_LEFT_4=99,22
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=60.157
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=176
=
PRIMER_SEQUENCE_ID=SSR_01307 Ps_contig_mira-and-tgicl-ass_franssen_12329
SEQUENCE=CTCTGATTTTTGAACGGTAGTGTCGGAGGATTTTGATTTGTTGAAACCAGACATGAAAACACGGAGCTTGGTGGCGGATTTCAAGAACGAGGCGGTGAACTGAGGTTTGGAGTTAGGTTCGGAGGAGTTCAGTTCCGACTGAACTTGGTGGTTGGTTAGAGGAGAAAGCGTAAAGCCGAGTTCAGTCTCAGACTCGTTTGGTTCTTCTTCTTCAGGTACAGTGAACTCTATGTCAAAGAAAGGCTCGTCTTCTTCTTCTCCTTCTTCGTCGGTAGTGGTTTCGACGTAG
TARGET=248,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3220
PRIMER_LEFT_PENALTY=0.157415
PRIMER_RIGHT_PENALTY=0.164560
PRIMER_LEFT_SEQUENCE=CTGAGGTTTGGAGTTAGGTTCG
PRIMER_RIGHT_SEQUENCE=GAAACCACTACCGACGAAGAAG
PRIMER_LEFT=99,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=60.157
PRIMER_RIGHT_TM=60.165
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.3930
PRIMER_LEFT_1_PENALTY=0.228464
PRIMER_RIGHT_1_PENALTY=0.164560
PRIMER_LEFT_1_SEQUENCE=GTGTCGGAGGATTTTGATTTGT
PRIMER_RIGHT_1_SEQUENCE=GAAACCACTACCGACGAAGAAG
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=281,22
PRIMER_LEFT_1_TM=60.228
PRIMER_RIGHT_1_TM=60.165
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.4134
PRIMER_LEFT_2_PENALTY=0.248820
PRIMER_RIGHT_2_PENALTY=0.164560
PRIMER_LEFT_2_SEQUENCE=GAGTTAGGTTCGGAGGAGTTCA
PRIMER_RIGHT_2_SEQUENCE=GAAACCACTACCGACGAAGAAG
PRIMER_LEFT_2=109,22
PRIMER_RIGHT_2=281,22
PRIMER_LEFT_2_TM=59.751
PRIMER_RIGHT_2_TM=60.165
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.4134
PRIMER_LEFT_3_PENALTY=0.248820
PRIMER_RIGHT_3_PENALTY=0.164560
PRIMER_LEFT_3_SEQUENCE=TAGGTTCGGAGGAGTTCAGTTC
PRIMER_RIGHT_3_SEQUENCE=GAAACCACTACCGACGAAGAAG
PRIMER_LEFT_3=113,22
PRIMER_RIGHT_3=281,22
PRIMER_LEFT_3_TM=59.751
PRIMER_RIGHT_3_TM=60.165
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=0.5170
PRIMER_LEFT_4_PENALTY=0.352395
PRIMER_RIGHT_4_PENALTY=0.164560
PRIMER_LEFT_4_SEQUENCE=AGTGTCGGAGGATTTTGATTTG
PRIMER_RIGHT_4_SEQUENCE=GAAACCACTACCGACGAAGAAG
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=281,22
PRIMER_LEFT_4_TM=60.352
PRIMER_RIGHT_4_TM=60.165
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_01308 Ps_contig_mira-and-tgicl-ass_franssen_12377
SEQUENCE=TAGAGATTTAGTAGTAGGAGTCGTTATTTGGGTGTGAAACATGATTGCCATTCTCAGAAGCCTAAGAAGAAAAGTGCACGAAACCGTAATCTTTCTGAGGAGGAATAATGCTTATGAGACTCTCAATGATCATTCTTTGTTCTTTTGCTTGAAGAGTTGAAGTTTCGTCTCTTGTTCCGCCCGATACAACCATATGAAGTCCACCACCGCCGCCTTTAATCCAGTTACTACCGCTACCACTACTACTACTACTATGACTATTACCGATACCGTAATTGCTCGTTTCGTCGAATGTGATTGTTGTTTCACTAACCAACCCTTGTASCCKATTTTGTTGCATAA
TARGET=239,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01309 Ps_contig_mira-and-tgicl-ass_franssen_12423
SEQUENCE=CGACAATCATAGTAACATATANACCTGCTGCTGCTGGTCACAACATTTTTAAAGAGAAGCTTACTCTCATTTCTAGTCACCATCCAACAGGGTAAAGCAACATGGCATATGTTTCTACTTCTGCTGCTTTATCAGAACCTGAATATGAAATGGTGAAACACATCATCGACATTAAAGACCTAGAAGAGTTAAGTTTAGCTGAGTGTAGCATCTACAATGTTCCTCACAATCTTAGAAAAGTTAAACAAGAAGCTTACACTCCTCAACTAATCTCAATCGGTCCCATTCACCTTGGAGAACAAGAACTTCAACCAATGCAACAGCACAAAAAAAAGGTACTTCCATTTCTTCTGGGAACGTTTATCTTCAACCAACCAACAATCCATGAAGAACTACAAACTCTATCTAGAAAACAAAGAACAAGAAATAAGAAAGTGTTATGCGGAGAAATTTCCTAATAT
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01310 Ps_contig_mira-and-tgicl-ass_franssen_12423
SEQUENCE=CGACAATCATAGTAACATATANACCTGCTGCTGCTGGTCACAACATTTTTAAAGAGAAGCTTACTCTCATTTCTAGTCACCATCCAACAGGGTAAAGCAACATGGCATATGTTTCTACTTCTGCTGCTTTATCAGAACCTGAATATGAAATGGTGAAACACATCATCGACATTAAAGACCTAGAAGAGTTAAGTTTAGCTGAGTGTAGCATCTACAATGTTCCTCACAATCTTAGAAAAGTTAAACAAGAAGCTTACACTCCTCAACTAATCTCAATCGGTCCCATTCACCTTGGAGAACAAGAACTTCAACCAATGCAACAGCACAAAAAAAAGGTACTTCCATTTCTTCTGGGAACGTTTATCTTCAACCAACCAACAATCCATGAAGAACTACAAACTCTATCTAGAAAACAAAGAACAAGAAATAAGAAAGTGTTATGCGGAGAAATTTCCTAATAT
TARGET=368,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7425
PRIMER_LEFT_PENALTY=0.248781
PRIMER_RIGHT_PENALTY=0.493727
PRIMER_LEFT_SEQUENCE=TGGAGAACAAGAACTTCAACCA
PRIMER_RIGHT_SEQUENCE=AGGAAATTTCTCCGCATAACAC
PRIMER_LEFT=292,22
PRIMER_RIGHT=455,22
PRIMER_LEFT_TM=59.751
PRIMER_RIGHT_TM=59.506
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.7425
PRIMER_LEFT_1_PENALTY=0.248781
PRIMER_RIGHT_1_PENALTY=0.493727
PRIMER_LEFT_1_SEQUENCE=TGGAGAACAAGAACTTCAACCA
PRIMER_RIGHT_1_SEQUENCE=GGAAATTTCTCCGCATAACACT
PRIMER_LEFT_1=292,22
PRIMER_RIGHT_1=454,22
PRIMER_LEFT_1_TM=59.751
PRIMER_RIGHT_1_TM=59.506
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.8133
PRIMER_LEFT_2_PENALTY=0.319597
PRIMER_RIGHT_2_PENALTY=0.493727
PRIMER_LEFT_2_SEQUENCE=TCAACTAATCTCAATCGGTCCC
PRIMER_RIGHT_2_SEQUENCE=AGGAAATTTCTCCGCATAACAC
PRIMER_LEFT_2=262,22
PRIMER_RIGHT_2=455,22
PRIMER_LEFT_2_TM=60.320
PRIMER_RIGHT_2_TM=59.506
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.8133
PRIMER_LEFT_3_PENALTY=0.319597
PRIMER_RIGHT_3_PENALTY=0.493727
PRIMER_LEFT_3_SEQUENCE=TCAACTAATCTCAATCGGTCCC
PRIMER_RIGHT_3_SEQUENCE=GGAAATTTCTCCGCATAACACT
PRIMER_LEFT_3=262,22
PRIMER_RIGHT_3=454,22
PRIMER_LEFT_3_TM=60.320
PRIMER_RIGHT_3_TM=59.506
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.8301
PRIMER_LEFT_4_PENALTY=0.336392
PRIMER_RIGHT_4_PENALTY=0.493727
PRIMER_LEFT_4_SEQUENCE=CCATTTCTTCTGGGAACGTTTA
PRIMER_RIGHT_4_SEQUENCE=AGGAAATTTCTCCGCATAACAC
PRIMER_LEFT_4=341,22
PRIMER_RIGHT_4=455,22
PRIMER_LEFT_4_TM=60.336
PRIMER_RIGHT_4_TM=59.506
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_01311 Ps_contig_mira-and-tgicl-ass_franssen_12433
SEQUENCE=CATTACACCAAGATCCATGTAGAAAAAAAGTGTGAGAGAGAGAAAAAAAAAGAAGAAAGTGGACAAGGTTTAAGGTAAGGAAATGAAGTGGTGTGTTATGTTATTATACCA
TARGET=34,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01312 Ps_contig_mira-and-tgicl-ass_franssen_12437
SEQUENCE=AAAACTTAATAATAAATAATCTTCATCCAATAAGCTCCCACAATCACTTGTGTCAAAATTCAAAGAATAGCTCAATGGATCATAGCAACATTGAAACGAACCTTGTCTGATTCGGCTATGTGATCTTTTCATGAGAAGTTTTCTCATCAGTTTTGCCATTAAACAACAACATAGATGATGATGATGATCTTGTAGTGGCCACAGTATTTTGTTGCCATGGTNATAGATCCACCTTTAANCTTCATGGTGGTAGG
TARGET=174,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4779
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=1.452353
PRIMER_LEFT_SEQUENCE=ACCTTGTCTGATTCGGCTATGT
PRIMER_RIGHT_SEQUENCE=CCATGAAGNTTAAAGGTGGATCT
PRIMER_LEFT=99,22
PRIMER_RIGHT=246,23
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=59.548
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.50
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=1.5219
PRIMER_LEFT_1_PENALTY=0.069526
PRIMER_RIGHT_1_PENALTY=1.452353
PRIMER_LEFT_1_SEQUENCE=ATCTTCATCCAATAAGCTCCCA
PRIMER_RIGHT_1_SEQUENCE=CCATGAAGNTTAAAGGTGGATCT
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=246,23
PRIMER_LEFT_1_TM=59.930
PRIMER_RIGHT_1_TM=59.548
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.50
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=6.75
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=229
PRIMER_PAIR_PENALTY_2=1.5989
PRIMER_LEFT_2_PENALTY=0.146525
PRIMER_RIGHT_2_PENALTY=1.452353
PRIMER_LEFT_2_SEQUENCE=CATTGAAACGAACCTTGTCTGA
PRIMER_RIGHT_2_SEQUENCE=CCATGAAGNTTAAAGGTGGATCT
PRIMER_LEFT_2=88,22
PRIMER_RIGHT_2=246,23
PRIMER_LEFT_2_TM=60.147
PRIMER_RIGHT_2_TM=59.548
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.50
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=1.7165
PRIMER_LEFT_3_PENALTY=0.264143
PRIMER_RIGHT_3_PENALTY=1.452353
PRIMER_LEFT_3_SEQUENCE=AGCTCAATGGATCATAGCAACA
PRIMER_RIGHT_3_SEQUENCE=CCATGAAGNTTAAAGGTGGATCT
PRIMER_LEFT_3=68,22
PRIMER_RIGHT_3=246,23
PRIMER_LEFT_3_TM=59.736
PRIMER_RIGHT_3_TM=59.548
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.50
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.75
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=179
PRIMER_PAIR_PENALTY_4=1.7227
PRIMER_LEFT_4_PENALTY=0.270338
PRIMER_RIGHT_4_PENALTY=1.452353
PRIMER_LEFT_4_SEQUENCE=CTTGTCTGATTCGGCTATGTGA
PRIMER_RIGHT_4_SEQUENCE=CCATGAAGNTTAAAGGTGGATCT
PRIMER_LEFT_4=101,22
PRIMER_RIGHT_4=246,23
PRIMER_LEFT_4_TM=60.270
PRIMER_RIGHT_4_TM=59.548
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.50
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_01313 Ps_contig_mira-and-tgicl-ass_franssen_12439
SEQUENCE=TATGGGAAGGTTAATCTTGTGATAAGGGATGGGAAGACAAAGTTTGAGACTCATGAGGTTGAGCCTCCTAGGAAAGATAAGTGGAAGACTAAGAAGAGGTTGAAGATTCAGAGGAAGAGGGAGAAGGAGAAGAGGAAAGCGGAAGAATAGGAGAGATCCTCGTCGACTTGGCGTTAAAGGGAAGAAGAAGAAACAAAAGTTTGCTAGTCCAGAAGAGAGAATCAAGTTCAAGATTGATAATGCGAGAGTACAAAGAAGCGTTGCTCATTAGAAAGACTCAAACG
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3247
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.301100
PRIMER_LEFT_SEQUENCE=GGATGGGAAGACAAAGTTTGAG
PRIMER_RIGHT_SEQUENCE=TGAGCAACGCTTCTTTGTACTC
PRIMER_LEFT=26,22
PRIMER_RIGHT=266,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.699
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=0.3442
PRIMER_LEFT_1_PENALTY=0.043085
PRIMER_RIGHT_1_PENALTY=0.301100
PRIMER_LEFT_1_SEQUENCE=AGAGGGAGAAGGAGAAGAGGAA
PRIMER_RIGHT_1_SEQUENCE=TGAGCAACGCTTCTTTGTACTC
PRIMER_LEFT_1=115,22
PRIMER_RIGHT_1=266,22
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=59.699
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=0.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.3442
PRIMER_LEFT_2_PENALTY=0.043085
PRIMER_RIGHT_2_PENALTY=0.301100
PRIMER_LEFT_2_SEQUENCE=AAGAGGGAGAAGGAGAAGAGGA
PRIMER_RIGHT_2_SEQUENCE=TGAGCAACGCTTCTTTGTACTC
PRIMER_LEFT_2=114,22
PRIMER_RIGHT_2=266,22
PRIMER_LEFT_2_TM=59.957
PRIMER_RIGHT_2_TM=59.699
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=0.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=153
PRIMER_PAIR_PENALTY_3=0.3442
PRIMER_LEFT_3_PENALTY=0.043085
PRIMER_RIGHT_3_PENALTY=0.301100
PRIMER_LEFT_3_SEQUENCE=GAAGAGGGAGAAGGAGAAGAGG
PRIMER_RIGHT_3_SEQUENCE=TGAGCAACGCTTCTTTGTACTC
PRIMER_LEFT_3=113,22
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=59.957
PRIMER_RIGHT_3_TM=59.699
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=0.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.3442
PRIMER_LEFT_4_PENALTY=0.043085
PRIMER_RIGHT_4_PENALTY=0.301100
PRIMER_LEFT_4_SEQUENCE=AGGAAGAGGGAGAAGGAGAAGA
PRIMER_RIGHT_4_SEQUENCE=TGAGCAACGCTTCTTTGTACTC
PRIMER_LEFT_4=111,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=59.699
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=0.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=156
=
PRIMER_SEQUENCE_ID=SSR_01314 Ps_contig_mira-and-tgicl-ass_franssen_12454
SEQUENCE=CCGATTCCGTCGTTGAATCGGAATGGTAAACCTAGTCTTTTTATCTCAGGCCGGTGAGGAAAGACGGGAGGTTAGGAATTAACGGAAGTTAGAATACACCGACCTGATATCCTTCATGCTTCACGGCATGATGGACGGTTGACTATTAGACAATCTAATACCAGATCGAGATAGTGAAAGAAGAAGGAAGAAAGTGAAGATAGAAAGAAGAAGATAGAAAGTAATTTGGAAGGGAAGGAAAGAAAGAAAGAAAGAAAGTAAAATATAGAAGTAATTTCGTAACGGTAGGTAGGGAAATTAAG
TARGET=238,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1567
PRIMER_LEFT_PENALTY=0.107763
PRIMER_RIGHT_PENALTY=1.048930
PRIMER_LEFT_SEQUENCE=CGGAAGTTAGAATACACCGACC
PRIMER_RIGHT_SEQUENCE=TCCCTACCTACCGTTACGAAAT
PRIMER_LEFT=82,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=58.951
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=1.1567
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=1.048930
PRIMER_LEFT_1_SEQUENCE=CGGAAGTTAGAATACACCGACC
PRIMER_RIGHT_1_SEQUENCE=ATTTCCCTACCTACCGTTACGA
PRIMER_LEFT_1=82,22
PRIMER_RIGHT_1=297,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=58.951
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=216
PRIMER_PAIR_PENALTY_2=1.2403
PRIMER_LEFT_2_PENALTY=0.107763
PRIMER_RIGHT_2_PENALTY=1.132541
PRIMER_LEFT_2_SEQUENCE=CGGAAGTTAGAATACACCGACC
PRIMER_RIGHT_2_SEQUENCE=TTCCCTACCTACCGTTACGAAAT
PRIMER_LEFT_2=82,22
PRIMER_RIGHT_2=295,23
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=60.133
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=1.2403
PRIMER_LEFT_3_PENALTY=0.107763
PRIMER_RIGHT_3_PENALTY=1.132541
PRIMER_LEFT_3_SEQUENCE=CGGAAGTTAGAATACACCGACC
PRIMER_RIGHT_3_SEQUENCE=AATTTCCCTACCTACCGTTACGA
PRIMER_LEFT_3=82,22
PRIMER_RIGHT_3=298,23
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=60.133
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=1.3189
PRIMER_LEFT_4_PENALTY=0.269968
PRIMER_RIGHT_4_PENALTY=1.048930
PRIMER_LEFT_4_SEQUENCE=CTGATATCCTTCATGCTTCACG
PRIMER_RIGHT_4_SEQUENCE=TCCCTACCTACCGTTACGAAAT
PRIMER_LEFT_4=103,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=59.730
PRIMER_RIGHT_4_TM=58.951
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_01315 Ps_contig_mira-and-tgicl-ass_franssen_12468
SEQUENCE=GTTACTCTTAGATGAGATTTGAGCTCTGACCAGTGCTGTTTACTCAACAGCTCCGAAATCGTTGATATGCAGAATGAGTTGGAGATTGGACCTTGAATACATTGAGAAAGTTTGTGAGTAGTCATTGCTCCTGGTGTTTTTTCCTTGAATCGCGGCGTGATTCAATCGGTGGTGCGGGGCTTTTTGCGGCGGCGGCGTGATTCAATCGCGGCGTGAATTCACTTAAACAAGAAAGAAAGATGCCAACAAAGTAAAA
TARGET=186,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01316 Ps_contig_mira-and-tgicl-ass_franssen_12468
SEQUENCE=GTTACTCTTAGATGAGATTTGAGCTCTGACCAGTGCTGTTTACTCAACAGCTCCGAAATCGTTGATATGCAGAATGAGTTGGAGATTGGACCTTGAATACATTGAGAAAGTTTGTGAGTAGTCATTGCTCCTGGTGTTTTTTCCTTGAATCGCGGCGTGATTCAATCGGTGGTGCGGGGCTTTTTGCGGCGGCGGCGTGATTCAATCGCGGCGTGAATTCACTTAAACAAGAAAGAAAGATGCCAACAAAGTAAAA
TARGET=229,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01317 Ps_contig_mira-and-tgicl-ass_franssen_12471
SEQUENCE=TGNTATTAGCCTTGATATGCTCCAATTAAGAACAATAGCCTTCTCACGACCGAGAATGGTAGAAGGGTAAGAGAGAGAGGGATCAAGGATTCTAAGATCACGTGCATTAATCTGAACCCTATGCATGATAGCATACTTGTCCACGTCAAGTATCGACCCTTGGCCCGTAGCATCAAACAGAATCCAACTTCTAGCAGACTGTGTCTTCTTCTTCGCAACAACCATAGCCTGTGGGTCCGCAGGGACAACGCTGCCATCTCGAGCCATCCCCTTACTCTATCCCTCCAACCACTTCAAATCACATACCACACCACACCACAACCTCAATTCAAAACAGCAATTCAAACTCCAAACTCTAA
TARGET=70,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2544
PRIMER_LEFT_PENALTY=0.137489
PRIMER_RIGHT_PENALTY=0.116923
PRIMER_LEFT_SEQUENCE=GAACAATAGCCTTCTCACGACC
PRIMER_RIGHT_SEQUENCE=TGCTATCATGCATAGGGTTCAG
PRIMER_LEFT=29,22
PRIMER_RIGHT=132,22
PRIMER_LEFT_TM=60.137
PRIMER_RIGHT_TM=60.117
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=0.2684
PRIMER_LEFT_1_PENALTY=0.137489
PRIMER_RIGHT_1_PENALTY=0.130883
PRIMER_LEFT_1_SEQUENCE=GAACAATAGCCTTCTCACGACC
PRIMER_RIGHT_1_SEQUENCE=TTGGATTCTGTTTGATGCTACG
PRIMER_LEFT_1=29,22
PRIMER_RIGHT_1=186,22
PRIMER_LEFT_1_TM=60.137
PRIMER_RIGHT_1_TM=60.131
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=0.3002
PRIMER_LEFT_2_PENALTY=0.137489
PRIMER_RIGHT_2_PENALTY=0.162674
PRIMER_LEFT_2_SEQUENCE=GAACAATAGCCTTCTCACGACC
PRIMER_RIGHT_2_SEQUENCE=GGAGGGATAGAGTAAGGGGATG
PRIMER_LEFT_2=29,22
PRIMER_RIGHT_2=285,22
PRIMER_LEFT_2_TM=60.137
PRIMER_RIGHT_2_TM=60.163
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.3002
PRIMER_LEFT_3_PENALTY=0.137489
PRIMER_RIGHT_3_PENALTY=0.162674
PRIMER_LEFT_3_SEQUENCE=GAACAATAGCCTTCTCACGACC
PRIMER_RIGHT_3_SEQUENCE=GAGGGATAGAGTAAGGGGATGG
PRIMER_LEFT_3=29,22
PRIMER_RIGHT_3=284,22
PRIMER_LEFT_3_TM=60.137
PRIMER_RIGHT_3_TM=60.163
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.3002
PRIMER_LEFT_4_PENALTY=0.137489
PRIMER_RIGHT_4_PENALTY=0.162674
PRIMER_LEFT_4_SEQUENCE=GAACAATAGCCTTCTCACGACC
PRIMER_RIGHT_4_SEQUENCE=TGGAGGGATAGAGTAAGGGGAT
PRIMER_LEFT_4=29,22
PRIMER_RIGHT_4=286,22
PRIMER_LEFT_4_TM=60.137
PRIMER_RIGHT_4_TM=60.163
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_01318 Ps_contig_mira-and-tgicl-ass_franssen_12471
SEQUENCE=TGNTATTAGCCTTGATATGCTCCAATTAAGAACAATAGCCTTCTCACGACCGAGAATGGTAGAAGGGTAAGAGAGAGAGGGATCAAGGATTCTAAGATCACGTGCATTAATCTGAACCCTATGCATGATAGCATACTTGTCCACGTCAAGTATCGACCCTTGGCCCGTAGCATCAAACAGAATCCAACTTCTAGCAGACTGTGTCTTCTTCTTCGCAACAACCATAGCCTGTGGGTCCGCAGGGACAACGCTGCCATCTCGAGCCATCCCCTTACTCTATCCCTCCAACCACTTCAAATCACATACCACACCACACCACAACCTCAATTCAAAACAGCAATTCAAACTCCAAACTCTAA
TARGET=305,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4558
PRIMER_LEFT_PENALTY=0.338015
PRIMER_RIGHT_PENALTY=1.117810
PRIMER_LEFT_SEQUENCE=GACTGTGTCTTCTTCTTCGCAA
PRIMER_RIGHT_SEQUENCE=GAGTTTGGAGTTTGAATTGCTG
PRIMER_LEFT=196,22
PRIMER_RIGHT=355,22
PRIMER_LEFT_TM=59.662
PRIMER_RIGHT_TM=58.882
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=10.5000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=1.7159
PRIMER_LEFT_1_PENALTY=0.598109
PRIMER_RIGHT_1_PENALTY=1.117810
PRIMER_LEFT_1_SEQUENCE=TTCTTCTTCGCAACAACCATAG
PRIMER_RIGHT_1_SEQUENCE=GAGTTTGGAGTTTGAATTGCTG
PRIMER_LEFT_1=205,22
PRIMER_RIGHT_1=355,22
PRIMER_LEFT_1_TM=59.402
PRIMER_RIGHT_1_TM=58.882
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=1.7159
PRIMER_LEFT_2_PENALTY=0.598109
PRIMER_RIGHT_2_PENALTY=1.117810
PRIMER_LEFT_2_SEQUENCE=CTTCTTCTTCGCAACAACCATA
PRIMER_RIGHT_2_SEQUENCE=GAGTTTGGAGTTTGAATTGCTG
PRIMER_LEFT_2=204,22
PRIMER_RIGHT_2=355,22
PRIMER_LEFT_2_TM=59.402
PRIMER_RIGHT_2_TM=58.882
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=1.7216
PRIMER_LEFT_3_PENALTY=0.603757
PRIMER_RIGHT_3_PENALTY=1.117810
PRIMER_LEFT_3_SEQUENCE=CTGTGTCTTCTTCTTCGCAACA
PRIMER_RIGHT_3_SEQUENCE=GAGTTTGGAGTTTGAATTGCTG
PRIMER_LEFT_3=198,22
PRIMER_RIGHT_3=355,22
PRIMER_LEFT_3_TM=60.604
PRIMER_RIGHT_3_TM=58.882
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=1.8367
PRIMER_LEFT_4_PENALTY=0.718884
PRIMER_RIGHT_4_PENALTY=1.117810
PRIMER_LEFT_4_SEQUENCE=GCAGACTGTGTCTTCTTCTTCG
PRIMER_RIGHT_4_SEQUENCE=GAGTTTGGAGTTTGAATTGCTG
PRIMER_LEFT_4=193,22
PRIMER_RIGHT_4=355,22
PRIMER_LEFT_4_TM=59.281
PRIMER_RIGHT_4_TM=58.882
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_01319 Ps_contig_mira-and-tgicl-ass_franssen_12480
SEQUENCE=TACATCATCAAGAACAATTAGGATTTGAGCCTGGCTCCCACCACATTCAAATTGCGGATTCCACTGAGGAACAACATAATTGCTATTCAAGTTTCCATTTCCCATGACGTATCCCCAAATCTTTGCCCTCAGCTGCTCCACATTAGGCGATTGTGAGACAGTTAGAAACAAAATTCTTTCTTTAAAATGACATCGAACTTCTTCATCTCTGCAAACCTCTCTAGCAAGAGTAGTTTTCCCAGATCCACCCATCCCACAAATCCCAACAACACACAAATCTTTTCTTCCAACAACCATCTCCATAACCTTCTTCTTCCCAAACTCCAAACCAATACTCAAATTAAAATTCCCGAAACTACCCTCATCTTCTTCATTAGTCCTAACAGCTTCCTCAACCCACCCACCACCACCAACCCCAATCTTCATCGACTCGAAAAACCTCTCTATCCTCCGGTTTGACTCATCAATCCGGTGGAACCGTTCACCCATATCAGACCGAAGCCGATGCACATCAGCAAGTATATAAGCCTGCAACGGAACCTGCAAAAACGTTGTCACACTTTCTTCAAGACTCTCCATCTTCTTAGCAAAGTTGAAAATTTCTATAAACATTCCAACGACTAGTAGCAAGAACTTGGTGAGAAAGCTCAACGCCGGAGCGGAGAATCTCCGATAGACGGTCGAGCTGAGACTGTCTCGGCGGCGGAAGTTCCGG
TARGET=401,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0567
PRIMER_LEFT_PENALTY=0.018372
PRIMER_RIGHT_PENALTY=0.038361
PRIMER_LEFT_SEQUENCE=AACCTTCTTCTTCCCAAACTCC
PRIMER_RIGHT_SEQUENCE=GATGAGTCAAACCGGAGGATAG
PRIMER_LEFT=303,22
PRIMER_RIGHT=464,22
PRIMER_LEFT_TM=59.982
PRIMER_RIGHT_TM=59.962
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=162
PRIMER_PAIR_PENALTY_1=0.0567
PRIMER_LEFT_1_PENALTY=0.018372
PRIMER_RIGHT_1_PENALTY=0.038361
PRIMER_LEFT_1_SEQUENCE=AACCTTCTTCTTCCCAAACTCC
PRIMER_RIGHT_1_SEQUENCE=ATGAGTCAAACCGGAGGATAGA
PRIMER_LEFT_1=303,22
PRIMER_RIGHT_1=463,22
PRIMER_LEFT_1_TM=59.982
PRIMER_RIGHT_1_TM=59.962
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=161
PRIMER_PAIR_PENALTY_2=0.0995
PRIMER_LEFT_2_PENALTY=0.061184
PRIMER_RIGHT_2_PENALTY=0.038361
PRIMER_LEFT_2_SEQUENCE=TCCATAACCTTCTTCTTCCCAA
PRIMER_RIGHT_2_SEQUENCE=GATGAGTCAAACCGGAGGATAG
PRIMER_LEFT_2=298,22
PRIMER_RIGHT_2=464,22
PRIMER_LEFT_2_TM=59.939
PRIMER_RIGHT_2_TM=59.962
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.0995
PRIMER_LEFT_3_PENALTY=0.061184
PRIMER_RIGHT_3_PENALTY=0.038361
PRIMER_LEFT_3_SEQUENCE=TCCATAACCTTCTTCTTCCCAA
PRIMER_RIGHT_3_SEQUENCE=ATGAGTCAAACCGGAGGATAGA
PRIMER_LEFT_3=298,22
PRIMER_RIGHT_3=463,22
PRIMER_LEFT_3_TM=59.939
PRIMER_RIGHT_3_TM=59.962
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.1141
PRIMER_LEFT_4_PENALTY=0.075753
PRIMER_RIGHT_4_PENALTY=0.038361
PRIMER_LEFT_4_SEQUENCE=AAATTCCCGAAACTACCCTCAT
PRIMER_RIGHT_4_SEQUENCE=ATGAGTCAAACCGGAGGATAGA
PRIMER_LEFT_4=343,22
PRIMER_RIGHT_4=463,22
PRIMER_LEFT_4_TM=60.076
PRIMER_RIGHT_4_TM=59.962
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_01320 Ps_contig_mira-and-tgicl-ass_franssen_12487
SEQUENCE=GGGGGGGGAAGTTGGAATATCTGTGAGAAATGGTTGGTTTAGGAGTGAGAGTAAGACTTCAACATTCCCTCTTTCTTCCTCACCAACCACTCACACGCTTCGCTCACTCTTCCTCCTCCATCTTCACTCTTTCAAATCCCCCAACAAGCCCTAGGTTTTATGTACTAGCTCACAGTAATAACCAAAATAGACACGTCCACGAAGATGAACCACCTTCTTCTTCTTCTTCTTCTTCCTCAGAACAACTTGAATGGATTCGGAATTCAGGAATTATATCTTCATGCTTGGTTGGTCTCTTAACCGGCGTCGCAGTTGTTCTCTTCAACAACACTGTTCATGAAATTCGTGATTTTTCGTGGGATGGGATTCCCGATCGAGGAGCTTCATGGTTAAGAGAAGCCCCTATTCAGGATACATGGAAAAGGGTTATACTTGTTCCAGCTTTTGGAGGACTTTTTGTCTCTCTCCTCAACCTCCTTTCCAAATTCCAAATTYCAATTTCATTTCCCCGCCCTTTCCTCAAAGCTATTGCAGCTTCCGTTAC
TARGET=214,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01321 Ps_contig_mira-and-tgicl-ass_franssen_12489
SEQUENCE=GGTGTTACGGTTGCATTTCGCTGGTAGAGATATGTATATGCTATCAGAGTTCAGTTTTTGAACAGTTAAGTCGCCAATTTTGATTTGATTGTTGTTGTTGCTGCAGTTTAATTTGATTTTGCAACCTTCTGAGAATCCGAATGGGTAGTTCACGGATTGTTCTCCGCATCTTCTGTTGCAGTGACTTGTTTGTTTTGATCAGCTAGTTTATTACTGTTATGAAGATTGTGATGATGATGAAGAATAAGAAAGCTTTGAATTGTGATGATGGCGATGATGATGATGATGAAGGAACACTTTCTGATGAAAAATATTGAAAATGGGGTTC
TARGET=89,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2420
PRIMER_LEFT_PENALTY=0.125411
PRIMER_RIGHT_PENALTY=0.116582
PRIMER_LEFT_SEQUENCE=TTGCATTTCGCTGGTAGAGATA
PRIMER_RIGHT_SEQUENCE=ACAATCCGTGAACTACCCATTC
PRIMER_LEFT=10,22
PRIMER_RIGHT=159,22
PRIMER_LEFT_TM=59.875
PRIMER_RIGHT_TM=60.117
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=150
PRIMER_PAIR_PENALTY_1=0.2420
PRIMER_LEFT_1_PENALTY=0.125411
PRIMER_RIGHT_1_PENALTY=0.116582
PRIMER_LEFT_1_SEQUENCE=TTGCATTTCGCTGGTAGAGATA
PRIMER_RIGHT_1_SEQUENCE=GAACAATCCGTGAACTACCCAT
PRIMER_LEFT_1=10,22
PRIMER_RIGHT_1=161,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.117
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.2552
PRIMER_LEFT_2_PENALTY=0.125411
PRIMER_RIGHT_2_PENALTY=0.129818
PRIMER_LEFT_2_SEQUENCE=TTGCATTTCGCTGGTAGAGATA
PRIMER_RIGHT_2_SEQUENCE=GAGAACAATCCGTGAACTACCC
PRIMER_LEFT_2=10,22
PRIMER_RIGHT_2=163,22
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=59.870
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=0.2552
PRIMER_LEFT_3_PENALTY=0.125411
PRIMER_RIGHT_3_PENALTY=0.129818
PRIMER_LEFT_3_SEQUENCE=TTGCATTTCGCTGGTAGAGATA
PRIMER_RIGHT_3_SEQUENCE=GGAGAACAATCCGTGAACTACC
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=164,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=59.870
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=0.2629
PRIMER_LEFT_4_PENALTY=0.125411
PRIMER_RIGHT_4_PENALTY=0.137489
PRIMER_LEFT_4_SEQUENCE=TTGCATTTCGCTGGTAGAGATA
PRIMER_RIGHT_4_SEQUENCE=AACAGAAGATGCGGAGAACAAT
PRIMER_LEFT_4=10,22
PRIMER_RIGHT_4=176,22
PRIMER_LEFT_4_TM=59.875
PRIMER_RIGHT_4_TM=60.137
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_01322 Ps_contig_mira-and-tgicl-ass_franssen_12489
SEQUENCE=GGTGTTACGGTTGCATTTCGCTGGTAGAGATATGTATATGCTATCAGAGTTCAGTTTTTGAACAGTTAAGTCGCCAATTTTGATTTGATTGTTGTTGTTGCTGCAGTTTAATTTGATTTTGCAACCTTCTGAGAATCCGAATGGGTAGTTCACGGATTGTTCTCCGCATCTTCTGTTGCAGTGACTTGTTTGTTTTGATCAGCTAGTTTATTACTGTTATGAAGATTGTGATGATGATGAAGAATAAGAAAGCTTTGAATTGTGATGATGGCGATGATGATGATGATGAAGGAACACTTTCTGATGAAAAATATTGAAAATGGGGTTC
TARGET=229,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5099
PRIMER_LEFT_PENALTY=0.038361
PRIMER_RIGHT_PENALTY=1.471585
PRIMER_LEFT_SEQUENCE=TCTGAGAATCCGAATGGGTAGT
PRIMER_RIGHT_SEQUENCE=GCCATCATCACAATTCAAAGC
PRIMER_LEFT=127,22
PRIMER_RIGHT=271,21
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=60.472
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.1000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=1.5882
PRIMER_LEFT_1_PENALTY=0.116582
PRIMER_RIGHT_1_PENALTY=1.471585
PRIMER_LEFT_1_SEQUENCE=ATGGGTAGTTCACGGATTGTTC
PRIMER_RIGHT_1_SEQUENCE=GCCATCATCACAATTCAAAGC
PRIMER_LEFT_1=140,22
PRIMER_RIGHT_1=271,21
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=60.472
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=1.5882
PRIMER_LEFT_2_PENALTY=0.116582
PRIMER_RIGHT_2_PENALTY=1.471585
PRIMER_LEFT_2_SEQUENCE=GAATGGGTAGTTCACGGATTGT
PRIMER_RIGHT_2_SEQUENCE=GCCATCATCACAATTCAAAGC
PRIMER_LEFT_2=138,22
PRIMER_RIGHT_2=271,21
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=60.472
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=1.5970
PRIMER_LEFT_3_PENALTY=0.125411
PRIMER_RIGHT_3_PENALTY=1.471585
PRIMER_LEFT_3_SEQUENCE=TTGCATTTCGCTGGTAGAGATA
PRIMER_RIGHT_3_SEQUENCE=GCCATCATCACAATTCAAAGC
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=271,21
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=60.472
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=1.6014
PRIMER_LEFT_4_PENALTY=0.129818
PRIMER_RIGHT_4_PENALTY=1.471585
PRIMER_LEFT_4_SEQUENCE=GGGTAGTTCACGGATTGTTCTC
PRIMER_RIGHT_4_SEQUENCE=GCCATCATCACAATTCAAAGC
PRIMER_LEFT_4=142,22
PRIMER_RIGHT_4=271,21
PRIMER_LEFT_4_TM=59.870
PRIMER_RIGHT_4_TM=60.472
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_01323 Ps_contig_mira-and-tgicl-ass_franssen_12489
SEQUENCE=GGTGTTACGGTTGCATTTCGCTGGTAGAGATATGTATATGCTATCAGAGTTCAGTTTTTGAACAGTTAAGTCGCCAATTTTGATTTGATTGTTGTTGTTGCTGCAGTTTAATTTGATTTTGCAACCTTCTGAGAATCCGAATGGGTAGTTCACGGATTGTTCTCCGCATCTTCTGTTGCAGTGACTTGTTTGTTTTGATCAGCTAGTTTATTACTGTTATGAAGATTGTGATGATGATGAAGAATAAGAAAGCTTTGAATTGTGATGATGGCGATGATGATGATGATGAAGGAACACTTTCTGATGAAAAATATTGAAAATGGGGTTC
TARGET=273,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01324 Ps_contig_mira-and-tgicl-ass_franssen_12521
SEQUENCE=AGTACGMRRGKMRTGGAGAGTATATAAACCAATCACATGCATTGATACTATTTCAAACCTTTCTCTCTCTCTCTCTCAATTTTAGTCTTAAGAGTGATCCAATAGTAGTGAAGAGTTTTGAGGGTAGAAAAAAAGAAAGAATCCATGGGAAGGCCTCCTTGCTGTGACAAAGTTGGCATCAAGAAAGGTCCATGGACACCTGAAGAAGATATCATCCTTGTTTCTTACATCCAAGAACATGGTCCAGGAAATTGGAGATCCGTTCCTACTAATACAGGGTTGTCAAGGTGCAGCAAAAGTTGCAGACTTAGATGGACAAATTATCTAAGGCCAGGAATCAAGAGAGGAAACTTCACTCCTCATGAAGAAGGAATGATTATYCATTTGCAAGCTCTTCTTGGTAACAAATGGGCAGCCATAGCTTCATACCTTCCACAAAGAACAGATAATGATATAAAGAATTATTGGAACACACATTTAAAGAAAAAGCTTAACAAATTTCAAGCTGCTGCTTTAGAGTCATCAAACTCAACAACTTATGTATCAAAAAGTTTTAGTGACAGAAGCTTAGATATTACCAACAATCATGCTTCGTCGCTAAAGCTTAATAGTAATAATCAAAATTCTCATCAATCTTCTTCATCAACATATGCTTCAAGCACTGAGAATATTTCAAGGCTCTTAGAAGGTTGGATGAGATCTTCACCAAACTCACAACAACAACAACAGATCAAAGGAAAATTACCAAATATGGTCAT
TARGET=62,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01325 Ps_contig_mira-and-tgicl-ass_franssen_12521
SEQUENCE=AGTACGMRRGKMRTGGAGAGTATATAAACCAATCACATGCATTGATACTATTTCAAACCTTTCTCTCTCTCTCTCTCAATTTTAGTCTTAAGAGTGATCCAATAGTAGTGAAGAGTTTTGAGGGTAGAAAAAAAGAAAGAATCCATGGGAAGGCCTCCTTGCTGTGACAAAGTTGGCATCAAGAAAGGTCCATGGACACCTGAAGAAGATATCATCCTTGTTTCTTACATCCAAGAACATGGTCCAGGAAATTGGAGATCCGTTCCTACTAATACAGGGTTGTCAAGGTGCAGCAAAAGTTGCAGACTTAGATGGACAAATTATCTAAGGCCAGGAATCAAGAGAGGAAACTTCACTCCTCATGAAGAAGGAATGATTATYCATTTGCAAGCTCTTCTTGGTAACAAATGGGCAGCCATAGCTTCATACCTTCCACAAAGAACAGATAATGATATAAAGAATTATTGGAACACACATTTAAAGAAAAAGCTTAACAAATTTCAAGCTGCTGCTTTAGAGTCATCAAACTCAACAACTTATGTATCAAAAAGTTTTAGTGACAGAAGCTTAGATATTACCAACAATCATGCTTCGTCGCTAAAGCTTAATAGTAATAATCAAAATTCTCATCAATCTTCTTCATCAACATATGCTTCAAGCACTGAGAATATTTCAAGGCTCTTAGAAGGTTGGATGAGATCTTCACCAAACTCACAACAACAACAACAGATCAAAGGAAAATTACCAAATATGGTCAT
TARGET=714,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01326 Ps_contig_mira-and-tgicl-ass_franssen_12522
SEQUENCE=CAACAACAACAACAGATCAAAGGAAATTACCAATATGGTCATCATCAAGAGTTTCAAAGCAATGGTGATGATAATGTTATTATTGATGATGATAATAATAGCTTTGAAAATAATGGTAATTTGTTAGTGAAGTCTGCTTCAGTTCCAGCTGATTTAAACCTTCAAGGCCATCATCATCATCATCATCATCACCAGCAGCAGCAGCAGAAAACTAGCAAAGATGAAATGGTTTCTCACGATCAAGTAGTTTGATTTCTTATTCTTTCATTTGAGAATCTTAACACGGT
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01327 Ps_contig_mira-and-tgicl-ass_franssen_12522
SEQUENCE=CAACAACAACAACAGATCAAAGGAAATTACCAATATGGTCATCATCAAGAGTTTCAAAGCAATGGTGATGATAATGTTATTATTGATGATGATAATAATAGCTTTGAAAATAATGGTAATTTGTTAGTGAAGTCTGCTTCAGTTCCAGCTGATTTAAACCTTCAAGGCCATCATCATCATCATCATCATCACCAGCAGCAGCAGCAGAAAACTAGCAAAGATGAAATGGTTTCTCACGATCAAGTAGTTTGATTTCTTATTCTTTCATTTGAGAATCTTAACACGGT
TARGET=169,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7291
PRIMER_LEFT_PENALTY=0.215215
PRIMER_RIGHT_PENALTY=0.513867
PRIMER_LEFT_SEQUENCE=CAAGAGTTTCAAAGCAATGGTG
PRIMER_RIGHT_SEQUENCE=CTTTGCTAGTTTTCTGCTGCTG
PRIMER_LEFT=45,22
PRIMER_RIGHT=219,22
PRIMER_LEFT_TM=59.785
PRIMER_RIGHT_TM=59.486
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=175
PRIMER_PAIR_PENALTY_1=0.8993
PRIMER_LEFT_1_PENALTY=0.385416
PRIMER_RIGHT_1_PENALTY=0.513867
PRIMER_LEFT_1_SEQUENCE=GAGTTTCAAAGCAATGGTGATG
PRIMER_RIGHT_1_SEQUENCE=CTTTGCTAGTTTTCTGCTGCTG
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=219,22
PRIMER_LEFT_1_TM=59.615
PRIMER_RIGHT_1_TM=59.486
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=1.5649
PRIMER_LEFT_2_PENALTY=0.215215
PRIMER_RIGHT_2_PENALTY=1.349728
PRIMER_LEFT_2_SEQUENCE=CAAGAGTTTCAAAGCAATGGTG
PRIMER_RIGHT_2_SEQUENCE=CTAGTTTTCTGCTGCTGCTGCT
PRIMER_LEFT_2=45,22
PRIMER_RIGHT_2=214,22
PRIMER_LEFT_2_TM=59.785
PRIMER_RIGHT_2_TM=61.350
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=1.6036
PRIMER_LEFT_3_PENALTY=0.215215
PRIMER_RIGHT_3_PENALTY=1.388340
PRIMER_LEFT_3_SEQUENCE=CAAGAGTTTCAAAGCAATGGTG
PRIMER_RIGHT_3_SEQUENCE=TCTTTGCTAGTTTTCTGCTGCT
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=220,22
PRIMER_LEFT_3_TM=59.785
PRIMER_RIGHT_3_TM=58.612
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=1.6909
PRIMER_LEFT_4_PENALTY=0.215215
PRIMER_RIGHT_4_PENALTY=1.475706
PRIMER_LEFT_4_SEQUENCE=CAAGAGTTTCAAAGCAATGGTG
PRIMER_RIGHT_4_SEQUENCE=TGCTAGTTTTCTGCTGCTGCT
PRIMER_LEFT_4=45,22
PRIMER_RIGHT_4=216,21
PRIMER_LEFT_4_TM=59.785
PRIMER_RIGHT_4_TM=60.476
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_01328 Ps_contig_mira-and-tgicl-ass_franssen_12522
SEQUENCE=CAACAACAACAACAGATCAAAGGAAATTACCAATATGGTCATCATCAAGAGTTTCAAAGCAATGGTGATGATAATGTTATTATTGATGATGATAATAATAGCTTTGAAAATAATGGTAATTTGTTAGTGAAGTCTGCTTCAGTTCCAGCTGATTTAAACCTTCAAGGCCATCATCATCATCATCATCATCACCAGCAGCAGCAGCAGAAAACTAGCAAAGATGAAATGGTTTCTCACGATCAAGTAGTTTGATTTCTTATTCTTTCATTTGAGAATCTTAACACGGT
TARGET=193,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.7779
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=2.760072
PRIMER_LEFT_SEQUENCE=TGCTTCAGTTCCAGCTGATTTA
PRIMER_RIGHT_SEQUENCE=CAAACTACTTGATCGTGAGAAACC
PRIMER_LEFT=134,22
PRIMER_RIGHT=250,24
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=59.240
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=117
PRIMER_PAIR_PENALTY_1=2.9753
PRIMER_LEFT_1_PENALTY=0.215215
PRIMER_RIGHT_1_PENALTY=2.760072
PRIMER_LEFT_1_SEQUENCE=CAAGAGTTTCAAAGCAATGGTG
PRIMER_RIGHT_1_SEQUENCE=CAAACTACTTGATCGTGAGAAACC
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=250,24
PRIMER_LEFT_1_TM=59.785
PRIMER_RIGHT_1_TM=59.240
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=3.1455
PRIMER_LEFT_2_PENALTY=0.385416
PRIMER_RIGHT_2_PENALTY=2.760072
PRIMER_LEFT_2_SEQUENCE=GAGTTTCAAAGCAATGGTGATG
PRIMER_RIGHT_2_SEQUENCE=CAAACTACTTGATCGTGAGAAACC
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=250,24
PRIMER_LEFT_2_TM=59.615
PRIMER_RIGHT_2_TM=59.240
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=3.2223
PRIMER_LEFT_3_PENALTY=0.462203
PRIMER_RIGHT_3_PENALTY=2.760072
PRIMER_LEFT_3_SEQUENCE=CTGATTTAAACCTTCAAGGCCA
PRIMER_RIGHT_3_SEQUENCE=CAAACTACTTGATCGTGAGAAACC
PRIMER_LEFT_3=148,22
PRIMER_RIGHT_3=250,24
PRIMER_LEFT_3_TM=60.462
PRIMER_RIGHT_3_TM=59.240
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=3.6236
PRIMER_LEFT_4_PENALTY=0.863548
PRIMER_RIGHT_4_PENALTY=2.760072
PRIMER_LEFT_4_SEQUENCE=AGTGAAGTCTGCTTCAGTTCCA
PRIMER_RIGHT_4_SEQUENCE=CAAACTACTTGATCGTGAGAAACC
PRIMER_LEFT_4=125,22
PRIMER_RIGHT_4=250,24
PRIMER_LEFT_4_TM=59.136
PRIMER_RIGHT_4_TM=59.240
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_01329 Ps_contig_mira-and-tgicl-ass_franssen_12525
SEQUENCE=GTCGGGGACACTTTCATTTCTCATTTCTCTTTTCTCTCTATAATCTTTCTCTTCCTAAAACCCTTCAAAAACAACCACAAACTCTCACTTCAATGGATTCTTCTCATTCAACTAATTCACCCATCACCACCATCCATTCAGACATAATCCAATCTCTCATCCTTTCCCGACTGGACGGCACATCTCTCACTTCTGCCGCCTCCACCGCTTCCCACCTCAACCGTCTCTGCACCGAACACCACCTCTGGCAAAAAATCTCCACCGCCACGTGGCCTTCTCTTAATGATCCTATCGCCAAGTCACTCATCTCCACCTTTCCATCATCCCACCGTTCAATTTTCTCTGACTCATTCCCCTCCGTCGATTACTCCTCTTCCTGTGCCTCTCCTTCAATCTCAACTGTATCATCATCATCCCTTACGAGAGAATTAATCTCCGCTGTAGATTTATACTACAAGGGCAAACCTGTCTTTTCGAAAATCCTACGCACGGACACTCATAAAGGATGGTTCTTATGTTCACCTCTATGGATTGAAATTCTCGAGCCGAACGAAGTTATTCAGACTTCTATAAAATTCGAGCAAAACGACGTCGTGGAGTGGCTTGAAGAAAAATCTAACGTTAAAGCTGGATTATGATTGAACCG
TARGET=404,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0453
PRIMER_LEFT_PENALTY=0.010294
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=ATCCTATCGCCAAGTCACTCAT
PRIMER_RIGHT_SEQUENCE=AAAAGACAGGTTTGCCCTTGTA
PRIMER_LEFT=285,22
PRIMER_RIGHT=473,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.0513
PRIMER_LEFT_1_PENALTY=0.016312
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=TACTCCTCTTCCTGTGCCTCTC
PRIMER_RIGHT_1_SEQUENCE=AAAAGACAGGTTTGCCCTTGTA
PRIMER_LEFT_1=365,22
PRIMER_RIGHT_1=473,22
PRIMER_LEFT_1_TM=60.016
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.1879
PRIMER_LEFT_2_PENALTY=0.016312
PRIMER_RIGHT_2_PENALTY=0.171564
PRIMER_LEFT_2_SEQUENCE=TACTCCTCTTCCTGTGCCTCTC
PRIMER_RIGHT_2_SEQUENCE=CTTTATGAGTGTCCGTGCGTAG
PRIMER_LEFT_2=365,22
PRIMER_RIGHT_2=503,22
PRIMER_LEFT_2_TM=60.016
PRIMER_RIGHT_2_TM=59.828
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=0.2212
PRIMER_LEFT_3_PENALTY=0.010294
PRIMER_RIGHT_3_PENALTY=0.210886
PRIMER_LEFT_3_SEQUENCE=ATCCTATCGCCAAGTCACTCAT
PRIMER_RIGHT_3_SEQUENCE=GTCTGAATAACTTCGTTCGGCT
PRIMER_LEFT_3=285,22
PRIMER_RIGHT_3=564,22
PRIMER_LEFT_3_TM=59.990
PRIMER_RIGHT_3_TM=59.789
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.2212
PRIMER_LEFT_4_PENALTY=0.010294
PRIMER_RIGHT_4_PENALTY=0.210886
PRIMER_LEFT_4_SEQUENCE=ATCCTATCGCCAAGTCACTCAT
PRIMER_RIGHT_4_SEQUENCE=AGTCTGAATAACTTCGTTCGGC
PRIMER_LEFT_4=285,22
PRIMER_RIGHT_4=565,22
PRIMER_LEFT_4_TM=59.990
PRIMER_RIGHT_4_TM=59.789
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_01330 Ps_contig_mira-and-tgicl-ass_franssen_12526
SEQUENCE=CTTCTTCTCTCCTCAACCTCCTCTCTCTCCTCTCTCTCTTCATCAAATAGAATTTCTCAAACCTTTCTTCATTCCTCAACATCCACCTTCTTCCTCTCACCCTTTTCCATCGCCCCTTGCAAAATTACCAAACTATCCCTCCCAACCATGTGTCTTCCATCCATATCCTCCATACTAAAACTCACCTCCCTCACGTGCATCTCTCCCCCAGGTTTCCCACAGCACGTGACCTTTATCGTGCACTGCACTAATCCCATAACCACAGCGTATAAAACCTCAAGCTCCCCCGTCAGCCAGTGACGTCTCACTGTTACCGGTAACCGGCTAGATAAGTTCACCGAACGCTTACGTGTCGGTTCAATCATAATCCAGCTTAACGTTAGATTTTCTTCAAG
TARGET=30,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9420
PRIMER_LEFT_PENALTY=0.824674
PRIMER_RIGHT_PENALTY=0.117281
PRIMER_LEFT_SEQUENCE=TCTCCTCAACCTCCTCTCTCTC
PRIMER_RIGHT_SEQUENCE=TGGTTATGGGATTAGTGCAGTG
PRIMER_LEFT=7,22
PRIMER_RIGHT=261,22
PRIMER_LEFT_TM=59.175
PRIMER_RIGHT_TM=59.883
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=255
PRIMER_PAIR_PENALTY_1=0.9420
PRIMER_LEFT_1_PENALTY=0.824674
PRIMER_RIGHT_1_PENALTY=0.117281
PRIMER_LEFT_1_SEQUENCE=TCTCCTCAACCTCCTCTCTCTC
PRIMER_RIGHT_1_SEQUENCE=TGTGGTTATGGGATTAGTGCAG
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=59.175
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.9420
PRIMER_LEFT_2_PENALTY=0.824674
PRIMER_RIGHT_2_PENALTY=0.117281
PRIMER_LEFT_2_SEQUENCE=TCTCTCCTCAACCTCCTCTCTC
PRIMER_RIGHT_2_SEQUENCE=TGGTTATGGGATTAGTGCAGTG
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=261,22
PRIMER_LEFT_2_TM=59.175
PRIMER_RIGHT_2_TM=59.883
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=1.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.9420
PRIMER_LEFT_3_PENALTY=0.824674
PRIMER_RIGHT_3_PENALTY=0.117281
PRIMER_LEFT_3_SEQUENCE=TCTCTCCTCAACCTCCTCTCTC
PRIMER_RIGHT_3_SEQUENCE=TGTGGTTATGGGATTAGTGCAG
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=263,22
PRIMER_LEFT_3_TM=59.175
PRIMER_RIGHT_3_TM=59.883
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.9984
PRIMER_LEFT_4_PENALTY=0.881127
PRIMER_RIGHT_4_PENALTY=0.117281
PRIMER_LEFT_4_SEQUENCE=CTCCTCAACCTCCTCTCTCTCC
PRIMER_RIGHT_4_SEQUENCE=TGTGGTTATGGGATTAGTGCAG
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=263,22
PRIMER_LEFT_4_TM=60.881
PRIMER_RIGHT_4_TM=59.883
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_01331 Ps_contig_mira-and-tgicl-ass_franssen_12530
SEQUENCE=TAGGCTAGGTTTTCTAAAAGCAATCAAGATGAAGAATTGTTGGATGATGATGATCAAGACCTCCATACCGTCAACACTTCTTCCGCCGCTCTCAACATCAACAACAACAATGTAAAGGTGGATGAACCTGGCAGAGGGAAAAGAGCGAACCCTCATCGTTCTAAGCATTCTGAGACTGAGCAGCGTAGAAGAAGCAAAATTAACGAAAGATTTCAGGTTCTGAGAGATCTTATACCTCAAAATGATCAAAAAAGAGATAAAGCTTCCTTTTTGTTGGAGGTTATTGAATATATCCAGTTCCTACAGGAAAAGTTACAAATTTATGAACATTCTTATGAAGGATGGAATCAGGAACCAAGTAAATTAATTCCATGGAGAAATCATCACGGACCTGCCGAAAACACTATAGATCCTTCTCGAGCTATTCCAAATGGGTCTGATCATGAAACACGTAACAATGTCTCTCTGTCATTGCCTAAAAACGTACAGAA
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01332 Ps_contig_mira-and-tgicl-ass_franssen_12530
SEQUENCE=TAGGCTAGGTTTTCTAAAAGCAATCAAGATGAAGAATTGTTGGATGATGATGATCAAGACCTCCATACCGTCAACACTTCTTCCGCCGCTCTCAACATCAACAACAACAATGTAAAGGTGGATGAACCTGGCAGAGGGAAAAGAGCGAACCCTCATCGTTCTAAGCATTCTGAGACTGAGCAGCGTAGAAGAAGCAAAATTAACGAAAGATTTCAGGTTCTGAGAGATCTTATACCTCAAAATGATCAAAAAAGAGATAAAGCTTCCTTTTTGTTGGAGGTTATTGAATATATCCAGTTCCTACAGGAAAAGTTACAAATTTATGAACATTCTTATGAAGGATGGAATCAGGAACCAAGTAAATTAATTCCATGGAGAAATCATCACGGACCTGCCGAAAACACTATAGATCCTTCTCGAGCTATTCCAAATGGGTCTGATCATGAAACACGTAACAATGTCTCTCTGTCATTGCCTAAAAACGTACAGAA
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1268
PRIMER_LEFT_PENALTY=0.077340
PRIMER_RIGHT_PENALTY=0.049498
PRIMER_LEFT_SEQUENCE=AGACCTCCATACCGTCAACACT
PRIMER_RIGHT_SEQUENCE=AATTTTGCTTCTTCTACGCTGC
PRIMER_LEFT=56,22
PRIMER_RIGHT=200,22
PRIMER_LEFT_TM=59.923
PRIMER_RIGHT_TM=60.049
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=0.3307
PRIMER_LEFT_1_PENALTY=0.077340
PRIMER_RIGHT_1_PENALTY=0.253332
PRIMER_LEFT_1_SEQUENCE=AGACCTCCATACCGTCAACACT
PRIMER_RIGHT_1_SEQUENCE=AGAATGCTTAGAACGATGAGGG
PRIMER_LEFT_1=56,22
PRIMER_RIGHT_1=170,22
PRIMER_LEFT_1_TM=59.923
PRIMER_RIGHT_1_TM=59.747
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=0.3310
PRIMER_LEFT_2_PENALTY=0.281487
PRIMER_RIGHT_2_PENALTY=0.049498
PRIMER_LEFT_2_SEQUENCE=ATGATCAAGACCTCCATACCGT
PRIMER_RIGHT_2_SEQUENCE=AATTTTGCTTCTTCTACGCTGC
PRIMER_LEFT_2=49,22
PRIMER_RIGHT_2=200,22
PRIMER_LEFT_2_TM=59.719
PRIMER_RIGHT_2_TM=60.049
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.3384
PRIMER_LEFT_3_PENALTY=0.288917
PRIMER_RIGHT_3_PENALTY=0.049498
PRIMER_LEFT_3_SEQUENCE=ACCTCCATACCGTCAACACTTC
PRIMER_RIGHT_3_SEQUENCE=AATTTTGCTTCTTCTACGCTGC
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=200,22
PRIMER_LEFT_3_TM=60.289
PRIMER_RIGHT_3_TM=60.049
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=0.3384
PRIMER_LEFT_4_PENALTY=0.288917
PRIMER_RIGHT_4_PENALTY=0.049498
PRIMER_LEFT_4_SEQUENCE=AAGACCTCCATACCGTCAACAC
PRIMER_RIGHT_4_SEQUENCE=AATTTTGCTTCTTCTACGCTGC
PRIMER_LEFT_4=55,22
PRIMER_RIGHT_4=200,22
PRIMER_LEFT_4_TM=60.289
PRIMER_RIGHT_4_TM=60.049
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_01333 Ps_contig_mira-and-tgicl-ass_franssen_12542
SEQUENCE=TCCGCTGGCCGTGCATCTTCTCGTGTTCCCCTCCATGTTCTTATAAATTCAAATTTTAAGACCATTTCTCTAAACTTCTTAATCCATTAGAGATGTATCAAAGTACAAGGTATTTCTGAAACCACAAATCTTTGAAACACGTACTTAAGCAGCTCCAGCTACTTCAAACTCGAGATCCCATAACCGTAGAAGAAGATCGAAGCATTTGAAGGCAACAATGGCGATATTGAAGTGAAAGAGAATTTGAATCTGAATGAATGAATGAATTCCGTCGGAGAGAGATGTGATAGTTTTGGGTTTAGGAGGAAGGTATGGAGAAAGCGATGGGGGTTAGAATAGAGAGTTTGAGAGAGAGTGTGTTGAGAGAGTAGTTAATTAAAACTTAAAAAGAGGTGAAAGATGAGGTGGTGAGTCACGTGGTCAGAGGGATCTCTCCGA
TARGET=251,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1152
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.095396
PRIMER_LEFT_SEQUENCE=CGAGATCCCATAACCGTAGAAG
PRIMER_RIGHT_SEQUENCE=GCTTTCTCCATACCTTCCTCCT
PRIMER_LEFT=170,22
PRIMER_RIGHT=321,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=0.1445
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.124726
PRIMER_LEFT_1_SEQUENCE=CGAGATCCCATAACCGTAGAAG
PRIMER_RIGHT_1_SEQUENCE=CCTTCCTCCTAAACCCAAAACT
PRIMER_LEFT_1=170,22
PRIMER_RIGHT_1=309,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=140
PRIMER_PAIR_PENALTY_2=0.2180
PRIMER_LEFT_2_PENALTY=0.122569
PRIMER_RIGHT_2_PENALTY=0.095396
PRIMER_LEFT_2_SEQUENCE=CCGTAGAAGAAGATCGAAGCAT
PRIMER_RIGHT_2_SEQUENCE=GCTTTCTCCATACCTTCCTCCT
PRIMER_LEFT_2=183,22
PRIMER_RIGHT_2=321,22
PRIMER_LEFT_2_TM=59.877
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=0.2390
PRIMER_LEFT_3_PENALTY=0.143555
PRIMER_RIGHT_3_PENALTY=0.095396
PRIMER_LEFT_3_SEQUENCE=AACTCGAGATCCCATAACCGTA
PRIMER_RIGHT_3_SEQUENCE=GCTTTCTCCATACCTTCCTCCT
PRIMER_LEFT_3=166,22
PRIMER_RIGHT_3=321,22
PRIMER_LEFT_3_TM=59.856
PRIMER_RIGHT_3_TM=60.095
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.2390
PRIMER_LEFT_4_PENALTY=0.143555
PRIMER_RIGHT_4_PENALTY=0.095396
PRIMER_LEFT_4_SEQUENCE=AGAAGATCGAAGCATTTGAAGG
PRIMER_RIGHT_4_SEQUENCE=GCTTTCTCCATACCTTCCTCCT
PRIMER_LEFT_4=190,22
PRIMER_RIGHT_4=321,22
PRIMER_LEFT_4_TM=59.856
PRIMER_RIGHT_4_TM=60.095
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_01334 Ps_contig_mira-and-tgicl-ass_franssen_12549
SEQUENCE=TTTTTAACATAGAAATAATAACCACATTCCACACAAAGCTCAAAAACAAAGCCAACCCTGATTAATCATATCATAAATATAGGTATATATACATAAACCATAACAACAATCAATATTTTTTTAAAAAGTTAAATATACATGTAGTTCCTACCAAATTCACAAATTTTCAAGTTTAATCCCTACAATTTTTCTTTCTTTCAATTTTAGCCCTAAACCTTGAATATTGAGAATTTTTGTTTGGTGTCAAAAGCTTATCAGAAAAGAGNGATATT
TARGET=188,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.0220
PRIMER_LEFT_PENALTY=1.027592
PRIMER_RIGHT_PENALTY=2.994441
PRIMER_LEFT_SEQUENCE=ACCACATTCCACACAAAGCTC
PRIMER_RIGHT_SEQUENCE=CTCAATATTCAAGGTTTAGGGC
PRIMER_LEFT=20,21
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=57.006
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=4.0826
PRIMER_LEFT_1_PENALTY=1.088154
PRIMER_RIGHT_1_PENALTY=2.994441
PRIMER_LEFT_1_SEQUENCE=CACATTCCACACAAAGCTCAAA
PRIMER_RIGHT_1_SEQUENCE=CTCAATATTCAAGGTTTAGGGC
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=227,22
PRIMER_LEFT_1_TM=61.088
PRIMER_RIGHT_1_TM=57.006
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=4.1097
PRIMER_LEFT_2_PENALTY=1.115242
PRIMER_RIGHT_2_PENALTY=2.994441
PRIMER_LEFT_2_SEQUENCE=ATAACCACATTCCACACAAAGC
PRIMER_RIGHT_2_SEQUENCE=CTCAATATTCAAGGTTTAGGGC
PRIMER_LEFT_2=17,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=58.885
PRIMER_RIGHT_2_TM=57.006
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=4.1292
PRIMER_LEFT_3_PENALTY=1.134770
PRIMER_RIGHT_3_PENALTY=2.994441
PRIMER_LEFT_3_SEQUENCE=CACAAAGCTCAAAAACAAAGCC
PRIMER_RIGHT_3_SEQUENCE=CTCAATATTCAAGGTTTAGGGC
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=227,22
PRIMER_LEFT_3_TM=61.135
PRIMER_RIGHT_3_TM=57.006
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=4.2457
PRIMER_LEFT_4_PENALTY=1.251261
PRIMER_RIGHT_4_PENALTY=2.994441
PRIMER_LEFT_4_SEQUENCE=CACATTCCACACAAAGCTCAA
PRIMER_RIGHT_4_SEQUENCE=CTCAATATTCAAGGTTTAGGGC
PRIMER_LEFT_4=22,21
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=59.749
PRIMER_RIGHT_4_TM=57.006
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=206
=
PRIMER_SEQUENCE_ID=SSR_01335 Ps_contig_mira-and-tgicl-ass_franssen_12554
SEQUENCE=AACGCAGAGTACGGCGGGGACCACNCGNGATAATAACTACACCCATATTTCAATATCCCTCTCTCACTTAATGTACAGAGAATAAAATAAAAAGTTAAAAATAAATCACAAAGAAAAACCACAACACAACGACACCGCTCCATCACCACCGTGAAGAAACTGCGTAAATCATGTATAAGATTCTTTCGTCATTTCATTAACTCCAGTCGGTACCATCTTCATTCCTTTTCATGATTTGTATGTTTATTTCCATGGTTTCATAACATTCCTTTACTTGATTTCTGGATCTAAGAAGGGCATGGTTTATTTTGTGTTAGATTTTGTTTTGAAAGGGAAGGTTTTCTCACCATGATAAARGAAAGAGTTTCGATGGTTATGTGTATAGGGATGAAGTGATTTTTCTTCTATATTATTCCTTGTTTAGTCTTCGAAGAGAGAGAGGAA
TARGET=432,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01336 Ps_contig_mira-and-tgicl-ass_franssen_12555
SEQUENCE=GATTTTTCTCTATATTATTCCTGTTTAGCTTCGAAGAGAGAGAGGAATGAGAGCATTTTGATTTGTTTCTATTCTTTTTCCTTCTTTACTGTTTTTTAGATTTGGGTCTAAAAGCCCCTTTGATGTTTTACACTTACGTGTCTATTGCTTACACTCCATTTTAGTGTGCAACCTATTGTAATTAGTGTTTCTATTTTCTTGTTGTTTATTTTAATTATTTAATTAATGAGTAATTAAGTTTTATTATTTGAGT
TARGET=35,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01337 Ps_contig_mira-and-tgicl-ass_franssen_12561
SEQUENCE=CTAAACATATCTCTTGTTATAGCTTGATTTTTTCGCTTATTCAGTAGACAAACAGATAATTTATTAATTTAACTCTTAATTATTATTATTATTTTATAAATTTTCTGCCATTA
TARGET=79,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01338 Ps_contig_mira-and-tgicl-ass_franssen_12605
SEQUENCE=GGTGAAGAATCTACTGGTTTGTGATTTTGCTCTATGCGGTTTGTGGCTGCGTTCTGCTGTTAGGAGCTGAAGTGGTGGTATGGCGGAAGCTGGTTGCAGAGTCGAAGGATGGGGAGTGAAGAAGAAGATTGAAAGAAAAGGTGAAGTGAAGTAATTGAAGAAGAGGGAAACGGAAATGGAGATTGAACCGAGTGAAAAACTACCAATCGAATGAGGATTCAACTGGTTAAATAGAGGATCAATCACAACTAACYCGGGCAAATTCTCGGGCAACCAGGTCAGTTAGTTAGTTAACAGTTATTTGGTGAAGGAGAAATTTTTTAGAATGCGGTTATTTGGTTCAGTTTCAGTTAGAAATGTTAGCTGGCGGTAGAATTCTGTTAGAAATCTGTTATGAGTTAATTATTATGAGGTTGAAATGGTTGGTAAGTGACTGTTTTGTGGTGTGTTGCAGG
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01339 Ps_contig_mira-and-tgicl-ass_franssen_12608
SEQUENCE=TAATGGCATTGTTGGAATAAGAAAACCCAGTTTTTATCAGGAAAATCACCAAATGGTTGTAGAACTAATTGGATCTTGCATGAATATCGTCTCGTCAACGTAGAAACTAGTTCTTCAAATTCAACTCAGAATTATGGAAATGAAATTGGAGATTGGCTTCTATGTCGATTATCGACGAAGGAAAAGAAATTTAGAATATGGAAGCACTAGTACTCATAATAATAATAATAATA
TARGET=216,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_01340 Ps_contig_mira-and-tgicl-ass_franssen_12609
SEQUENCE=AAATTTAGAATATGGAAGCACTAGTACTCTATTAATAATAATAATAATAACAATAACAATAATGCTACTCCGAATTCGRGATTAATGTTTGATTTTATGATGGTAAACAAACAAGGCATGTTCTTCTACTTCATCTTCATGTTCAAGTTCAAGTAACAACAACATTGAGGTCTCTTCAAATCTACAAGATCATGAACAAGATTATGCTGAACTATTTTTAATAKMAGTNAATTATTTTAATATTATGTTAATTTCTTTGT
TARGET=33,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01341 Ps_contig_mira-and-tgicl-ass_franssen_12619
SEQUENCE=TGTTGCCAACTTGATAAAAGCCACCTTGGTCTATTATTCCTATTCAGATATGAAATCAAATTGAAGAGAGGAAGGTAAGGATGATGATGATTTCAGATGCATGGTTTTGGAGATGAAAATGGTTGTAACCATTGTGACTTTAACTCCTTTTGTGCYTAAACGTTTGCTAAATTGAAGCATTGGGTTTATGTGACCTTGGCTTGGATATGGAACCATTAAAACNTGCATATTACTACACTTTCTCTCTTCCTATTTTTTTATTCTA
TARGET=80,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01342 Ps_contig_mira-and-tgicl-ass_franssen_12649
SEQUENCE=GAGGAACAGGGAGGAAGAAGAAGAATGAGGAAGATGGTGATGGTGATGATGGYGGTGCTTTTTCTTCTTCTCCTATGGTTTTTAAACCGCTTAATTTGGATTTTGATCAGTGTACACCTGTTAGAGGGAGGAATTTTGTTGGAAAAGATGGAACGCCGGTTAGATGTTCGGTTGGTAGGTGGGTCGTTTTTAGGGAAAATGAGTGA
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01343 Ps_contig_mira-and-tgicl-ass_franssen_12718
SEQUENCE=TAAGGAAGGAAGGAAAGGAAAGRGAAGAKKARTTWGTATTTTTACGTATGCTTTTGTCTTATCCCTGGGAGAAAGATAAACATTACAAAATGGTTTATCAATTGGAGAAAAAGTTTTTTCCTCATCAGTGTTTGGATAAAGCTTTTCTTCAACCAGGTCAATCAAAATCAAATTCAAATTCAGTTTCAGTTTCAATTTCAAATTCAGTTTCAGTTTCAAATTTGAATGAGAATGAGAATGTGAATGAGAATGAGATTGCTAGGGTTAGAAACAAGAAAGTTGGTGTTATTGGTGGGGAGAATGAAGATGAGAAGGTTGGTGATGGTGATAACAAGTTAGTGTTTTTCGAGGAGAATGTGAAAGGGGAAGAAGACAGAAAAAAGGGTGAGAATAAAGATCATAGTGAGAAGAAGGTTGAGGATTTTTTTAAGGGTTTGAAGAAAGATGTTGAAGTTGTCGAGCCGTTTTTTTCTTCAAGGAGGACTGGTCTTCCTCCTGTTTGGGATTCTCCACACGGGACAGTGCTTTTGATTAACAAACCTAAAGGTTGGACTTCATTCACAGTTTGTGGAAAGCTGCGTCGCCTTGTTAAAGTGAAAAAGGTTGGCCATGCTGGAACTCTTGATCCAATGGCAACTGGTTTATTGATTGTTTGTGTTGGCAAATCAACAAAACTGGTGGATAGATATCAAGGGATGATTAAGGGTTATAGTGGGGTTTTCCGCTTAGGGGAGGCTACTTCAACATGGGATGCTGATTCACCCGTCATTCAGCGTGAGCCATGGGAGCACATCAAAGATGAGGACATAAAGAAAAGTGCCTTGTCCTTTTGTGGGGAGATTTGGCAAGTTCCTCCTATGTTCTCTGCTATTAAAGTTGGAGGTGAAAAGATGTATGAGAAAGCAAGGAGAGGAGAAAGCATTGAACTTTCGCCTAGACGAATTTCAATTTTCCAATTTGACATAGAGCGTAGCTTGGACGACAGACAAAATTTGATTTTTAGAGTGACTTGCTCTAAAGGGACATATATTCGGTCACTCTGTGCAGATTTTGGGAAGGCTCTTGGCAGCTGTGCCCATTTAACGGCTCTCCGAAGAGATTCAATTGGACAATATTTAGCTGATGATGCATGGGAGTTTCAAGAGCTGGAAGAGTCTATCACCAAAACTTATCTTTAACTTCTCTGTTTCTCCACCCTGTAACATGCAGTTTTCTTTTTTGGTAAACATGTTTATGTACCAATTCTTAAGCATATTATGGCCCTCTCTGAAATTTTTTAACTTTCAGAATTCATTTTTGAGTTGGTATGTAAAATTGAGGGAAAGGGTCTGTGAAATTCATTCATTCATTATTTATATACCTTCAATGTCCCTCAACTTACATCTTTGGTGGTTGTTGTCGCTGTAAAATACTTTCAATGGAATCCTTAT
TARGET=2,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01344 Ps_contig_mira-and-tgicl-ass_franssen_12718
SEQUENCE=TAAGGAAGGAAGGAAAGGAAAGRGAAGAKKARTTWGTATTTTTACGTATGCTTTTGTCTTATCCCTGGGAGAAAGATAAACATTACAAAATGGTTTATCAATTGGAGAAAAAGTTTTTTCCTCATCAGTGTTTGGATAAAGCTTTTCTTCAACCAGGTCAATCAAAATCAAATTCAAATTCAGTTTCAGTTTCAATTTCAAATTCAGTTTCAGTTTCAAATTTGAATGAGAATGAGAATGTGAATGAGAATGAGATTGCTAGGGTTAGAAACAAGAAAGTTGGTGTTATTGGTGGGGAGAATGAAGATGAGAAGGTTGGTGATGGTGATAACAAGTTAGTGTTTTTCGAGGAGAATGTGAAAGGGGAAGAAGACAGAAAAAAGGGTGAGAATAAAGATCATAGTGAGAAGAAGGTTGAGGATTTTTTTAAGGGTTTGAAGAAAGATGTTGAAGTTGTCGAGCCGTTTTTTTCTTCAAGGAGGACTGGTCTTCCTCCTGTTTGGGATTCTCCACACGGGACAGTGCTTTTGATTAACAAACCTAAAGGTTGGACTTCATTCACAGTTTGTGGAAAGCTGCGTCGCCTTGTTAAAGTGAAAAAGGTTGGCCATGCTGGAACTCTTGATCCAATGGCAACTGGTTTATTGATTGTTTGTGTTGGCAAATCAACAAAACTGGTGGATAGATATCAAGGGATGATTAAGGGTTATAGTGGGGTTTTCCGCTTAGGGGAGGCTACTTCAACATGGGATGCTGATTCACCCGTCATTCAGCGTGAGCCATGGGAGCACATCAAAGATGAGGACATAAAGAAAAGTGCCTTGTCCTTTTGTGGGGAGATTTGGCAAGTTCCTCCTATGTTCTCTGCTATTAAAGTTGGAGGTGAAAAGATGTATGAGAAAGCAAGGAGAGGAGAAAGCATTGAACTTTCGCCTAGACGAATTTCAATTTTCCAATTTGACATAGAGCGTAGCTTGGACGACAGACAAAATTTGATTTTTAGAGTGACTTGCTCTAAAGGGACATATATTCGGTCACTCTGTGCAGATTTTGGGAAGGCTCTTGGCAGCTGTGCCCATTTAACGGCTCTCCGAAGAGATTCAATTGGACAATATTTAGCTGATGATGCATGGGAGTTTCAAGAGCTGGAAGAGTCTATCACCAAAACTTATCTTTAACTTCTCTGTTTCTCCACCCTGTAACATGCAGTTTTCTTTTTTGGTAAACATGTTTATGTACCAATTCTTAAGCATATTATGGCCCTCTCTGAAATTTTTTAACTTTCAGAATTCATTTTTGAGTTGGTATGTAAAATTGAGGGAAAGGGTCTGTGAAATTCATTCATTCATTATTTATATACCTTCAATGTCCCTCAACTTACATCTTTGGTGGTTGTTGTCGCTGTAAAATACTTTCAATGGAATCCTTAT
TARGET=1336,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01345 Ps_contig_mira-and-tgicl-ass_franssen_12721
SEQUENCE=CACCATACCGAATACGTTGGTCTTCCGGTAAAGCCTTTTGAATTCTGTGATCTTGGCGTGCTCCTTATCATAGGCAAGTCTTGCAAATGGATCTGAAAGAATGGCATAAGCATCATTAAGAATGATGGCCATGTCATGACCAGAAGGACCTGCAATATCAGGATGGCATCGTTTCTGAAGAGATCGATACGCGGTCTTGATCTGTGACTGATCACACGAGCTATCAATTCCTAGAAGATCATACAAGTCAAAATCCAACATGGATGATGAAAAACTTGATGATGATGATGCCTTGCATGTGAGAGTAGAGAAATTGGATTTAGAAAAACAAGACTTGTTGTAGAGATTTGAAGTTTTGGGAGTGAAGAAAGGAAGAGATGAAGATGAAACACTAGCCATGTTTATGGTTTGAGTTTGAGAACAAGGTGAGGCAACAATAGGATAAGACATCATCGTACCTTCCCGCGTACTCTGTCGTTGTATTACCTTTTACTTTTTGCTTA
TARGET=277,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1739
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.142170
PRIMER_LEFT_SEQUENCE=GACCAGAAGGACCTGCAATATC
PRIMER_RIGHT_SEQUENCE=CTCTTCCTTTCTTCACTCCCAA
PRIMER_LEFT=137,22
PRIMER_RIGHT=376,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=59.858
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.5000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.2453
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.213575
PRIMER_LEFT_1_SEQUENCE=GACCAGAAGGACCTGCAATATC
PRIMER_RIGHT_1_SEQUENCE=TCTTCCTTTCTTCACTCCCAAA
PRIMER_LEFT_1=137,22
PRIMER_RIGHT_1=375,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=60.214
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.5000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.2962
PRIMER_LEFT_2_PENALTY=0.031769
PRIMER_RIGHT_2_PENALTY=0.264444
PRIMER_LEFT_2_SEQUENCE=GACCAGAAGGACCTGCAATATC
PRIMER_RIGHT_2_SEQUENCE=CTTCCTTTCTTCACTCCCAAAA
PRIMER_LEFT_2=137,22
PRIMER_RIGHT_2=374,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=59.736
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.5000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.3162
PRIMER_LEFT_3_PENALTY=0.174021
PRIMER_RIGHT_3_PENALTY=0.142170
PRIMER_LEFT_3_SEQUENCE=ATATCAGGATGGCATCGTTTCT
PRIMER_RIGHT_3_SEQUENCE=CTCTTCCTTTCTTCACTCCCAA
PRIMER_LEFT_3=154,22
PRIMER_RIGHT_3=376,22
PRIMER_LEFT_3_TM=59.826
PRIMER_RIGHT_3_TM=59.858
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.3200
PRIMER_LEFT_4_PENALTY=0.177853
PRIMER_RIGHT_4_PENALTY=0.142170
PRIMER_LEFT_4_SEQUENCE=AATATCAGGATGGCATCGTTTC
PRIMER_RIGHT_4_SEQUENCE=CTCTTCCTTTCTTCACTCCCAA
PRIMER_LEFT_4=153,22
PRIMER_RIGHT_4=376,22
PRIMER_LEFT_4_TM=60.178
PRIMER_RIGHT_4_TM=59.858
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_01346 Ps_contig_mira-and-tgicl-ass_franssen_12722
SEQUENCE=AGAATTCAAAGGCTTTACCGGAAGACCAACGTATTCGGTATGGTGTGGTTCAGAAAGTGAGGAGAGGGCTGTATTTGTTGATGAAATAAAATGTGTTGGATGCTTAAAATGTGCTTTGTTAGCTGAAAAGACTTTTGCTATAGAATCAGTTTATGGAAGAGCTAGGGTTGTTTCTCAGTGGGCTGATTCTGAACCTAAAATTGATGAGGCAATACAAGCTTGTCCTGTCAACTGCAYTTCAGTTGTTGAGAGATCCAACCTTGCTGCTCTAGAGTTCTTGATGTCGAAGCAGACACGCGGCATAGTAAGAGTAGGTGCGAGTAATACAGCAGGTGTTCGCGTCTCGAACATATTTGTGGATGTGGAGAAGTTTCAGACTAGATTCCAACAGACAATGGAAAAAGCTTCCAAGTACTCTAAGGAGACGGATCTCCAAAGAGAATCAAGAATGTCAGCAATTCAAGCAATAAGATCAATTTCCAATTGGTTATACTGGCATCCACATAGTGCAAGTGTTAACAAAAGCATAACAAGAGTTGTGCATCAACTACCTGATCCGAATCTTAATAAGCTTAGAGATGCAGCTGCAAAGAGAAAACTAAGAGATTCAAACAAAACCAACAATCAAACTCCTTTAAACTCCATTCATCACGAGGAATATTGGACTCCATCGACAATCGATCAAACTAGTACTATTAGTAGTATTACTACTCCAACACCAATTGAGAAGCCTTCGTATGCGAAAGGAAAGAAACAAAGAAAGAAAG
TARGET=756,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_01347 Ps_contig_mira-and-tgicl-ass_franssen_12746
SEQUENCE=GAAGCAAAGARRRGGAGATAGCAGCACTCTGTCCCATTTCATCACTTTCATTTCACTCTGTTTCTCACTTTGCTTCTTCTTCTTCATCCTTCCCAGAAATGTCACTTACTCCCTTCTTCACAACAACACTAACCTTCTTTCTCATCATCACAGAAGTAACCTCTCACTACTCTTCTTCGTCTTCTCAATCACCACCTTCTCAACAAACCTCACCTTCTTCACCGAATGGCGAT
TARGET=73,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01348 Ps_contig_mira-and-tgicl-ass_franssen_12747
SEQUENCE=GGTTAAACTTTTGCAATTATTGTAACTATTACGACTTCTCAAAACCTAAACAAACTCTCACTCTCTCGCTCACTGTTACTACTCTATATCATTGTTATCTTCTTCTTTCTTTCTGGTTGGTTTGGATTTCGGTTTCATTAATTCATTTTTACAGTTACTTGTGGATTTTGTGAGGAAGTTATGGCAGGAGGTGGCGGTGGTGGTGGAGCTCCGGCGCCGAAAGCGGATGAGCCACAGCCTCATCCACCGAAAGATCAGCTACCTAATGTTTCTTACTGCATAACAAGTCCTCCTCCTTGGCCGGAGGCCATACTTCTTGGTTTCCAACATTATCTTGTGAT
TARGET=103,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0787
PRIMER_LEFT_PENALTY=0.069737
PRIMER_RIGHT_PENALTY=0.008930
PRIMER_LEFT_SEQUENCE=CAAACTCTCACTCTCTCGCTCA
PRIMER_RIGHT_SEQUENCE=CCAAGGAGGAGGACTTGTTATG
PRIMER_LEFT=50,22
PRIMER_RIGHT=299,22
PRIMER_LEFT_TM=59.930
PRIMER_RIGHT_TM=59.991
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=0.1923
PRIMER_LEFT_1_PENALTY=0.069737
PRIMER_RIGHT_1_PENALTY=0.122569
PRIMER_LEFT_1_SEQUENCE=CAAACTCTCACTCTCTCGCTCA
PRIMER_RIGHT_1_SEQUENCE=GTTGGAAACCAAGAAGTATGGC
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=327,22
PRIMER_LEFT_1_TM=59.930
PRIMER_RIGHT_1_TM=59.877
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.2001
PRIMER_LEFT_2_PENALTY=0.069737
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=CAAACTCTCACTCTCTCGCTCA
PRIMER_RIGHT_2_SEQUENCE=CAAGGAGGAGGACTTGTTATGC
PRIMER_LEFT_2=50,22
PRIMER_RIGHT_2=298,22
PRIMER_LEFT_2_TM=59.930
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.2967
PRIMER_LEFT_3_PENALTY=0.069737
PRIMER_RIGHT_3_PENALTY=0.226971
PRIMER_LEFT_3_SEQUENCE=CAAACTCTCACTCTCTCGCTCA
PRIMER_RIGHT_3_SEQUENCE=AGGAGGAGGACTTGTTATGCAG
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=59.930
PRIMER_RIGHT_3_TM=59.773
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.4263
PRIMER_LEFT_4_PENALTY=0.069737
PRIMER_RIGHT_4_PENALTY=0.356592
PRIMER_LEFT_4_SEQUENCE=CAAACTCTCACTCTCTCGCTCA
PRIMER_RIGHT_4_SEQUENCE=GGAGGAGGACTTGTTATGCAGT
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=295,22
PRIMER_LEFT_4_TM=59.930
PRIMER_RIGHT_4_TM=59.643
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_01349 Ps_contig_mira-and-tgicl-ass_franssen_12756
SEQUENCE=TTATCACATGAACTACTATACATAACCAAATCAGAATHAAAACTGAAAGCTATGATGTTATATATATATGGTGAATTTGATGTTTTCTGAAACGAAGATCGATATGGTGAACCAAATCAGAACAATGATTTAACTATACATAACGAAAACTGAAAGCTATGTTTTCGCAAACACATAACAATGATAGAATAACCAAATCAGAACAAGAATTTAACCATACATAATACTTGGTTGAAGTAGGAGTCACCTCGTCATGAACACCACCTGCATTGAAAGAAACTCAAAATCAAAGAAAAAAGGAAAACGAAATGTAGAAAACAAAACCCTAATAATATCTGAACGGGAGAGAAAAGTCAAAAAAAATACAGAAAACAAAACCCTAATACTTGGTTGAAATAGGAGTCACGTCGTCACGAACACCACCTGCATTGAAGAGAAAACAAAACCCTAAACTCAAAATCAAAGAGAAAAGGAAATGCAGAAAAGCAAAACCCYAACAACAATGTCTGAACGGCAGCGAAGAAAGACGAGACAGGGAAAAAAATACTC
TARGET=59,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01350 Ps_contig_mira-and-tgicl-ass_franssen_12779
SEQUENCE=TAGAACAACAAACAAACACTCAGATAGTAAGAATCAACAATGGAGGCTTCGAATAAAATAGGTCAACGCCAAATTGAARGAAGTGAGAGAGTAATATGAAKAWATGGATGTGAA
TARGET=7,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01351 Ps_contig_mira-and-tgicl-ass_franssen_12831
SEQUENCE=GTTTTTCCCTTTCATTTCGAGTATTTTGAGCATGAGGAATAATATCTTGGCCACCATAAAGTATTGATGAGCTGAGATTACATGGTTCAATTCTTTGGTCTTGATATATGTAACTCTTATCTGCAGTTTGTATTTTTTGAGATCCACCACCACAGGCCTTGAAAATGACATCAGGTGTTCCAAGTATAGAGTCCCATTTCTCCTTGATAGCTTTGTCCCTCACTTCATTAGGGGATGGAGAAGAAGAGGAAGATGAGTGTGTCTCTTTGGAGCCAGAGAGGTCAGAAACAAATGAAGAAGAAGAATCATTTTGGTTACTTTTTCCTTTCCATTTTCTTATTATATTGTTATCAAAATACACTACTTCAACTTTTGGT
TARGET=294,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01352 Ps_contig_mira-and-tgicl-ass_franssen_12847
SEQUENCE=TACAATACAAAAACGAAAAAATTCTTCAGAAACTGTTACATACATGAATGTCTTACCTCTMMAAAAAATGAGGTTAGAAACCAAAACAACTGCTCATTATTCGAGTACAACTACTCTAGACCTTTTCTGTGTCTCCTCATCATTTGATAGATTAAATACTAATCTTACACACTTCATACCAGAAAGTTCAAATTATATGAATGAACATGTGATATTAGATTCCACTAAAATTTGAGGATGACTCTGTTTTCATCTATGCAATATGCCCCGCTAAGGTAAGGATTTTGACATGCTTCTTCTGCCTCAACTCATGAGGAACTGGTCCGAAAACTCTGGTCCCAATAGGCTGCCCTTGTTTGTCAACAAGGACGACAGCATTGTCGTCAAACTTGACCTCACTGCCATCACACCTCCCCTTGGGCATTGCTGCACGAACAACTACGGCAAATACAGTTGATCCCTTCTTCACTTTTCCATTAGGATGTGCTTCCTTCACTGATGCTTTTATCATATCACCTAATCTTGCTCCTTTCTTCCCTTTCAACGCGTGTATGCACATCACCTTTTTAGCTCCAGAGTTGTCCACAACCTTCAGAACAGTCCTCATCTGTATGAAGGTTCGCTGTTGCTGAGAAAAATAATTGCTGCATGTCATCTCATGTGTCGTGGTAAATAGACCAGGGGAACTGTTGCTTAATCCACCGAACAGAGAACGGCCCACACGAGAACATCTGGAAGCAAAGGTTGCAGCCATTTTCAAAAGTTACAATCTTCTATATTCCCTGCGAGGAGAGAGAGAAGCTATGAAGTCGGAGAAGAGGGGGAAATGAGTGTACGCAGCAGAACCAAAGTCACGAAAGATGCAGTCGCATC
TARGET=790,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01353 Ps_contig_mira-and-tgicl-ass_franssen_12848
SEQUENCE=CTAATACAAAAACGTAAAAAATTTCTTCAAGAAACTGTTACATACMTATGAATRKTCCTTACCTCTAACAAAAAAATRGAGGTTAGAAACCAAAACAACTGCTCATTATTCGAGTACAACTACTCTAGACCTTTTCTGTGTCTCCTCATCATTTGATAGATTAAATACTAATCTTACACACTTCATACCAGAAAGTTCAAATTATATGAATGAACATGTGATATTAGATTCCACTAAAATTTGAGGGTGACTCTGTTTTCATCTATGCAATATGCCCGGCTAAGGTAAGGATCTTGACATGCTTCTTCTGCCTCAACTCATGAGGAACTGGTCCGAAAACTCTGGTCCCAATAGGCTGCCCTTGTTTGTCAACAAGGACAACAGCATTGTCGTCAAACTTGACCTCACTGCCATCACACCTCCCCTTGGGCATTGCTGCACGAACAACTACGGCAAATACAGTTGATCCCTTCTTCACTTTTCCATTAGGATGTGCTTCCTTCACTGATGCTTTTATCATATCACCTAGTCTTGCTCCTTTCTTCCCTTTCAACGCGTGTATGCACATCACCTTTTTAGCTCCAGAGTTGTCCACAACCTTCAGAACAGTCCTCATCTGTATGAAGGTTCGCTGTTGCTGAGAAAAATAATTGCTGCATGTCATCTCATGTGACGTGGTAAATAGACCAGGGGAACTGTTGCTTAATCCACCGAACAGAGAACGGCCCACACGAGAACATCTGGAAGCAAAGGTTGCAGCCATTTTCAAAAGTTACAATCTTCTATATTCCCTGCGAGGAGAGAGAGAAGCTATGAAGCCGGAGAAGAGGGGGAAATGAGTGTACGCAGCAGAACCAAAGTCACGAAAGATGCAGTCRCMYCGA
TARGET=799,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01354 Ps_contig_mira-and-tgicl-ass_franssen_12856
SEQUENCE=TTGTAATAGATAACTGGTTTATTGGAATTGAAATCCCACATGCCTCCTTCCTTAAGATACTCAATATACCTTGCAAATTCAGGCCTAGCTGTTGTTTTCTTTTCCCTACGAAAATCCAAGAAGTTGAAGAGTGGATGACTCTTCTTCTTACTACTACTACTATTASCCTTCTTTGTAGGGTTTCTTTGTAGCTTTATATCCCTAAAAGTTGAACCTAGAACCATTTTTGTTGAAGAATACGATGGTTTAGAAGGCATGGTTGTACTTCTCTTGAACTCCTCATGCAAAAGTGTTGTGGTTGCAGATTCTTTCCCCAACCTCTCATACCTCCCAGTTCCAAAACCTCTACTCATGGATCTAAACATGACTAAATGTGTGAAGAAA
TARGET=149,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01355 Ps_contig_mira-and-tgicl-ass_franssen_12879
SEQUENCE=GATATACTGATCAATGAAGAAGAAGAAACTATGAATATATATCACTTGGATGTGATACTTTGCCAGTTCTCAAGGGTAGAACCCCAGTTCAATGTTATGCTGATTTCATGCGTGCTTTCAGAGACAATTTCAAGCACCTTCTTGGGGATACCATTAAGTGGAAATCCAAGTTGGAATGGGACCAGCTGGTGAGTTTACGTTACTCTTCATA
TARGET=16,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01356 Ps_contig_mira-and-tgicl-ass_franssen_12895
SEQUENCE=GKAGATGATATTTTAGTTACGAAATTCGTTTCTGTAAAGTTCGTTTTGGTCAAGCTGCGCGGGCATGCATGCATAGGTGGTGAGATGAAAGTCCTTGCGGAATTTGGATCTGCTCAACTGCTTAATAACTATTCACCGATATGCAAGTTGTATAAGCTGGATTATTGTTAATTTCCTTGCTGCCAATTACCAAGTTCCCTAAAAGTTAAACTCAGTTTTTTTAAGCAAAAAAGTTGTGTATCAACTTTTTT
TARGET=63,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01357 Ps_contig_mira-and-tgicl-ass_franssen_12905
SEQUENCE=CGATGCCACCGAGAATTCTTCATCTTCTTCTTCTGACGCGAGTCTTGCTGTTGTTGTTCTTCAACAACACCAAGCGGAGATTCATTTGCTTGGAAAAGGAACCACCACTCGTTGTTCCAAAATCGAAGCCTCTTCACATTCAACGTAAAAAAT
TARGET=23,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01358 Ps_contig_mira-and-tgicl-ass_franssen_12906
SEQUENCE=CTCGCGTCAGAAGAAGAAGATGAAGTAATTCTCGGTGGCATCGGATAAAGCTTCAACGGCGTGTGGACTCCACAACTTTTTCCGAAGTTCAAAGGGCTTGTTTCTTTCGAGAGTTAAAGTGTGGAACGAACCAGCGGCGACGTGTTGATTTGATTCAAGGTTGCGGCCATGGACGCGGAGGGTGGAAGAGTCTTTGTGAAAGTCGCGGCTT
TARGET=9,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01359 Ps_contig_mira-and-tgicl-ass_franssen_12910
SEQUENCE=GSAAGAAAACAAGAGTATATATAATATGATATTTCAAGTCAATACTCCTAATACTAATGCATTTACAACCCAAAGGATCTCACAACTTATAAGAGAAGAGATCAATGTCATCATAATATAAACATTCAGTAAAAAATAATAATATAAATGAACATAACCTGATCAAAGAATAGATGAAACCTCTATACAGAGAGATATTACATATAAAGAACAAATTCTGATTTACAGGGCTATATATGTGTGTGTGTCTTGTCCATATAAATAGCAGGTGTGTACCCTATCCACATTTTTAAGAATATGATGCAGCTAGCAGTGCTGCTCCAATGCCTGATCCATCTTCGGTGACATTTAGAGTTACATGCTTTGCAATATCTTCCCCCAGTATTTCGTTTAAAGCTTCATGCAAGTACTCCCTAAACAATGTATAGCTCGAGTATAATCCTCCTTCGATTGCCACAACTGTTCTTTTCATTTTTATATCGCTCCTACTTCGTCCTCCTGTGATCCCACCACTCCCATCCCGACCAATCTTCTTCCCT
TARGET=238,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01360 Ps_contig_mira-and-tgicl-ass_franssen_12922
SEQUENCE=CGTTTTTTTTAATTTTTTCTATTGCATTTGAAAGTACTTCATAGGATGTAGTGAACAATGCTTATTTTGGACAAGTTCCACTATTACATGATATATATATATAGTAATCAACAACATGCAACTTATACAACAAATGCACATATATTTTGCTAGTGAATCCTGCAAGTTTGAACATCAATAGTTCAAACCAAAATAGTGCACTCCCTAGTTTTTTTATTGGTATATACTAAAACTTAAAAGTTTTCAAAATTCTCACGAATAAGGTTTAAATCCAAATAAACCTCTAGTATTCTATTTCTCTAAAACATCAAATTTTCGAACAAATGAAAGTAAATAATTTACAGTTGCACTAAACTTCAATTTTCACAGATTGTGTGGCTCCAACTATTTGTGTCGACTCTTATGTCTTGTGGACAGGCAGGAAGGACATAGAAAATTACCCAATATAGTGCGTAATGCAGTGATCCAAAGGCACAAGTTATGTTCCTACAACCTCCAAACTCTGGTCCAATTTTCAGTGATCCAAGAGAGAGAATGCTACGAGTGAATCAGGAACTCTTCAATTCCTA
TARGET=91,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.6637
PRIMER_LEFT_PENALTY=2.199575
PRIMER_RIGHT_PENALTY=0.464167
PRIMER_LEFT_SEQUENCE=GCTTATTTTGGACAAGTTCCAC
PRIMER_RIGHT_SEQUENCE=CTTGCAGGATTCACTAGCAAAA
PRIMER_LEFT=59,22
PRIMER_RIGHT=165,22
PRIMER_LEFT_TM=57.800
PRIMER_RIGHT_TM=59.536
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=2.9499
PRIMER_LEFT_1_PENALTY=2.199575
PRIMER_RIGHT_1_PENALTY=0.750366
PRIMER_LEFT_1_SEQUENCE=GCTTATTTTGGACAAGTTCCAC
PRIMER_RIGHT_1_SEQUENCE=GGGAGTGCACTATTTTGGTTTG
PRIMER_LEFT_1=59,22
PRIMER_RIGHT_1=204,22
PRIMER_LEFT_1_TM=57.800
PRIMER_RIGHT_1_TM=60.750
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=3.0652
PRIMER_LEFT_2_PENALTY=2.199575
PRIMER_RIGHT_2_PENALTY=0.865663
PRIMER_LEFT_2_SEQUENCE=GCTTATTTTGGACAAGTTCCAC
PRIMER_RIGHT_2_SEQUENCE=GGAGTGCACTATTTTGGTTTGA
PRIMER_LEFT_2=59,22
PRIMER_RIGHT_2=203,22
PRIMER_LEFT_2_TM=57.800
PRIMER_RIGHT_2_TM=59.134
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=3.1308
PRIMER_LEFT_3_PENALTY=2.199575
PRIMER_RIGHT_3_PENALTY=0.931205
PRIMER_LEFT_3_SEQUENCE=GCTTATTTTGGACAAGTTCCAC
PRIMER_RIGHT_3_SEQUENCE=AGGGAGTGCACTATTTTGGTTT
PRIMER_LEFT_3=59,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=57.800
PRIMER_RIGHT_3_TM=59.069
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=3.1555
PRIMER_LEFT_4_PENALTY=2.199575
PRIMER_RIGHT_4_PENALTY=0.955963
PRIMER_LEFT_4_SEQUENCE=GCTTATTTTGGACAAGTTCCAC
PRIMER_RIGHT_4_SEQUENCE=CAAACTTGCAGGATTCACTAGC
PRIMER_LEFT_4=59,22
PRIMER_RIGHT_4=169,22
PRIMER_LEFT_4_TM=57.800
PRIMER_RIGHT_4_TM=59.044
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=111
=
PRIMER_SEQUENCE_ID=SSR_01361 Ps_contig_mira-and-tgicl-ass_franssen_12955
SEQUENCE=AAGATTCGACGAAAAGGAAAGAGTTAAGGTAATAGAAAAAGCACAACAAACCATCACAATAATGGAAGAGCGAGGTTTAGGGGACAAGTAAGTTTTTTGGTGGCAACAATATTGGAATGGTAGACATAGTATATGGATGTCTTGGTCATTGGTTAGAAGGGTTGGAAGAAATTGTTGGGATCAAATTAATTGAGGAAAAAAATTTTCCTTGCATGCATGCATGGATTCAAAACTTTAAGCAAGTTCCTTTGATTAAAGAAAACCTTCCAGATTATGAGAAATTGTTGCTCCATCTTCAGTGGCGTAGGCAGGAATATGTTATATAGTTTCATCAATATGAAAAAGTGGTTGGAAATTAGG
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6927
PRIMER_LEFT_PENALTY=0.095629
PRIMER_RIGHT_PENALTY=0.597078
PRIMER_LEFT_SEQUENCE=CATCACAATAATGGAAGAGCGA
PRIMER_RIGHT_SEQUENCE=ATAACATATTCCTGCCTACGCC
PRIMER_LEFT=51,22
PRIMER_RIGHT=321,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.403
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=11.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.8181
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=0.597078
PRIMER_LEFT_1_SEQUENCE=GTCATTGGTTAGAAGGGTTGGA
PRIMER_RIGHT_1_SEQUENCE=ATAACATATTCCTGCCTACGCC
PRIMER_LEFT_1=144,22
PRIMER_RIGHT_1=321,22
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=59.403
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=11.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.8244
PRIMER_LEFT_2_PENALTY=0.227356
PRIMER_RIGHT_2_PENALTY=0.597078
PRIMER_LEFT_2_SEQUENCE=TGGATGTCTTGGTCATTGGTTA
PRIMER_RIGHT_2_SEQUENCE=ATAACATATTCCTGCCTACGCC
PRIMER_LEFT_2=133,22
PRIMER_RIGHT_2=321,22
PRIMER_LEFT_2_TM=60.227
PRIMER_RIGHT_2_TM=59.403
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=11.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.8560
PRIMER_LEFT_3_PENALTY=0.258908
PRIMER_RIGHT_3_PENALTY=0.597078
PRIMER_LEFT_3_SEQUENCE=TTGGTCATTGGTTAGAAGGGTT
PRIMER_RIGHT_3_SEQUENCE=ATAACATATTCCTGCCTACGCC
PRIMER_LEFT_3=141,22
PRIMER_RIGHT_3=321,22
PRIMER_LEFT_3_TM=59.741
PRIMER_RIGHT_3_TM=59.403
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=11.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.9416
PRIMER_LEFT_4_PENALTY=0.344527
PRIMER_RIGHT_4_PENALTY=0.597078
PRIMER_LEFT_4_SEQUENCE=TCTTGGTCATTGGTTAGAAGGG
PRIMER_RIGHT_4_SEQUENCE=ATAACATATTCCTGCCTACGCC
PRIMER_LEFT_4=139,22
PRIMER_RIGHT_4=321,22
PRIMER_LEFT_4_TM=60.345
PRIMER_RIGHT_4_TM=59.403
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_01362 Ps_contig_mira-and-tgicl-ass_franssen_12962
SEQUENCE=ATCCACAGAGCTGTAGAACTCTTCTGGAATTGATGGCTCTKGATAAACACCTAATTTTGAGGAAAATGTACCAAAGAAAATTTGGCGGAGGCCCCTCCATGCTACAGAATCTTATTGGAAGCAAATCGAGTCAAGAGAAGTGGATTTGCGCAGATCATTTCTTTGCCAATCCTACAAGTACTTCCTTGTTGTAGCGAAAATATATGGGTATTGGATATGCAAAGTAGATTAATATCATGTATATATATAGGCAATTTCAGATACTGATATTAAAGTYCATATTGGAAGAATCATAACTTAGGTAGCTTTTTGGACKCCATGTTCTGTTCTGACTTGAGAATATGTTCAATGTTGAAATTGATGAGTTGAGTAAGATTTCAAAGGGTCACAATCAACCCAGTGTTATCAATTAG
TARGET=240,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01363 Ps_contig_mira-and-tgicl-ass_franssen_12964
SEQUENCE=TAAACATAACTAACGTAACTAAAAGTTAAATTAATTAACAATAAATTATTTATCCATCGATGAGGTGATAAACAAATAAAAATAATTCAAAAAAATGCGACACAAAAAGAAAATAAAATAAAATTACAAAAATAGCAAAATGCCTCAAAAAGACAAGGCAACCAAGAGAGCCACGCCACCCAAAACAACCGGAGCCCCGGAAATTGATCTTGAACCCGAATCATCGCCGATAGGACCCGGTGAAGAAGTTTCTGGTGAAGAACTCGACGGTGCAGTTCCAGGCGGCGATTTGCTAGACGGAAGTGTCGCCGGTGAATCTGCCGGAGTAGCCGAACTTGAAGGTATACCTCCCGAAGAAGCCTCTGGTGGAGAAGAAACTGATGGACCTTCTGTTACCGTTGGGGCCCCAACAGGACTACTTACCGGAGCCACCGCTGCAGTTGGCGGAGGAGTGGACGGGGATTGAACCGGTGGCGAAGACACATTGGTGGATGGGCTGATAGCCGGGGAAACCAAAGCCGGTGGCTGAACAGTCGGAGAGTTCGCCGGCGATGGAGTAGACCCAGATGGAGAGATCGCCGGAGTTTGTGAAGGCGGAGATAAAGTTGGAGACTTTGCAGGGGATGCTTTAGAAGCCGTGGGAGACTGTACCGGAGAAACCGAGACCGTAGAAGGTGGTTTCGCCGGTGGTAGAGAAACCGGTGACTTTGGAGAAGAAGCCGGAGCATTGTCTGCAAGAACCAAAACCGAGGATGTTGTGAAGAACATAGAAAGTAGTAAGAGTGAAAGCAAGTTATTCATTTTTGGAGTTTTTGAGAATTCGGAAAGTACGAGGATGAATGAAAAAAATAGGTAGCAAGTATGTCGGAAAATAGTTACAACAATGTTGGAATTCTAAGTGATCGTGTTTGTGTGTCTCCCBGAC
TARGET=110,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01364 Ps_contig_mira-and-tgicl-ass_franssen_12974
SEQUENCE=ACTGAGATTATTGTAATCTTCTTTGCTTCCTATAAATAATCATCAGAACTGACACGATGCAAGAGTATGGTCTCATTGTCTTCTTCATCATAAACATATAAGCCCAGATCCTCAGATCTCTCTAAATAATCAATAGTTACATGCCCAGTACCCTGGTCATCCCATTTTCCATCTTCATTCAGACGGTACACCTTGACCCGCTGCATCGAACTAGCGTTGGTCTGTGATTTCTCTGGCGCTGGCGCTCCCATCACAGCTCTTCAAGATTCAAAGCAATAACGAAATACACAATCTCGGGAACACTAAATTAGGGTTTTGAGACGAAGGAGAATGGGAGAGAAAACAATTGCAGTGAAGAGATTGAGCTTCGTCGGATCAAGTATCAACAAGAAACCCTAACGGCCATTGACGTAGCAGCGTGAAGTTTCTCTTCAATCTCTCTCTACCCTCTYCTCTCTCTCTCCTCC
TARGET=453,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01365 Ps_contig_mira-and-tgicl-ass_franssen_12975
SEQUENCE=GGKATTCAACTAAACGGTTACTTCATTTTTCACTTCTCTCTCTCTCTCTCAAACAACAACAACACCTTCATTCTTGTAGGTCAGGGTGCATTCATATTTCAACGTGTGTGAGGCCCAAACCATTAACCCCATTGAGCTATAAGCTATGCATACCCCAAAGACAAGAAGTGGTTCTTCTTCTGAAGTGACTCAGAAGGTTTCTGCACGTGCGGTACGTCAAATGAGGACGACTACTATAA
TARGET=35,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01366 Ps_contig_mira-and-tgicl-ass_franssen_12975
SEQUENCE=GGKATTCAACTAAACGGTTACTTCATTTTTCACTTCTCTCTCTCTCTCTCAAACAACAACAACACCTTCATTCTTGTAGGTCAGGGTGCATTCATATTTCAACGTGTGTGAGGCCCAAACCATTAACCCCATTGAGCTATAAGCTATGCATACCCCAAAGACAAGAAGTGGTTCTTCTTCTGAAGTGACTCAGAAGGTTTCTGCACGTGCGGTACGTCAAATGAGGACGACTACTATAA
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01367 Ps_contig_mira-and-tgicl-ass_franssen_12978
SEQUENCE=CTAAGAATACGAGCCCCATCCCTACCAAGACAAYGGACTAAGCGCRACCTTTCAAGAAGAATCTCAGATTGAAGCTGAAGTCGTTTACCGAATTCTCAATGGGAAATCGCATTCGCTGAAGCCTAATTCTGGTGAAACGGTTATGATTTGTGAAAGTAGCATTGCTGTTGGGTTTCATGCGGAAGAAGAAGGGGAGTATATTGTGTGGGAATGGCATGGTCACATTCCGAGGTACACCGAAGACCATGAGTTTTCTCTTGAGTATATCTATGGGAATTACTTTCAGAGGATTGTGCCTGAGGAGTGTACTCCCACACCGCCTCGAGTAGATGCAGCGGTTGATATGGGCTTGAAGGACTTGTTTGATGGTGTTGTCAATCCCGCCCCTGGTAGGATTCTTCATCGCAATCTCAACATTGGTTCCACTCCTCCCAGGCTCTAAGAATTTCGTCATTGGATTTTAAAGTTTAAAGGATCGCAGGCAGAGGTCATAGTCATCATCTCCTTCCTTTTGTGAAGCAAAGATAGTTTTCCGATTTTCCCAGTACAGATCTCAAGAATAAGCCTGCATCTCTATCCTTGTTTGACTTTCAAATCATACTTGTAAAGGATGCAAGACATTAGGTTTTTAATTTAATTTTAGTTTATTTCTTTGTCGTTGGGTGGTAATTAATTAATTAGTACTTAA
TARGET=667,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01368 Ps_contig_mira-and-tgicl-ass_franssen_12979
SEQUENCE=GTCGGGGGTTAAAACCCGTTTCCGTTTCACAAAACCCAGCCAATCATTTTCAGGGAGAACTTTCATCAAACACCATGAGCTCCCCTCCAAACAACTCTTCCTCCTCCGTCAACCGCCTCGACACCATGTTCCTCCACCATCTAATGCGCAGCCTTCAGGTTCAAAATTCTGACAACCAACTTACCGACGAGGATTTCATTTCCGACGACGACGAAGAAGAACTAGAATACGAGCCCCATCCCTACCAAGACAACGGACTAAGCG
TARGET=203,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4711
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=0.396594
PRIMER_LEFT_SEQUENCE=CCATGTTCCTCCACCATCTAAT
PRIMER_RIGHT_SEQUENCE=TTAGTCCGTTGTCTTGGTAGGG
PRIMER_LEFT=123,22
PRIMER_RIGHT=260,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=60.397
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=138
PRIMER_PAIR_PENALTY_1=0.5481
PRIMER_LEFT_1_PENALTY=0.151533
PRIMER_RIGHT_1_PENALTY=0.396594
PRIMER_LEFT_1_SEQUENCE=GGGAGAACTTTCATCAAACACC
PRIMER_RIGHT_1_SEQUENCE=TTAGTCCGTTGTCTTGGTAGGG
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=260,22
PRIMER_LEFT_1_TM=59.848
PRIMER_RIGHT_1_TM=60.397
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.6118
PRIMER_LEFT_2_PENALTY=0.215225
PRIMER_RIGHT_2_PENALTY=0.396594
PRIMER_LEFT_2_SEQUENCE=ATGTTCCTCCACCATCTAATGC
PRIMER_RIGHT_2_SEQUENCE=TTAGTCCGTTGTCTTGGTAGGG
PRIMER_LEFT_2=125,22
PRIMER_RIGHT_2=260,22
PRIMER_LEFT_2_TM=60.215
PRIMER_RIGHT_2_TM=60.397
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.7583
PRIMER_LEFT_3_PENALTY=0.074466
PRIMER_RIGHT_3_PENALTY=0.683827
PRIMER_LEFT_3_SEQUENCE=CCATGTTCCTCCACCATCTAAT
PRIMER_RIGHT_3_SEQUENCE=GGATGGGGCTCGTATTCTAGTT
PRIMER_LEFT_3=123,22
PRIMER_RIGHT_3=240,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=60.684
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=0.7886
PRIMER_LEFT_4_PENALTY=0.074466
PRIMER_RIGHT_4_PENALTY=0.714145
PRIMER_LEFT_4_SEQUENCE=CCATGTTCCTCCACCATCTAAT
PRIMER_RIGHT_4_SEQUENCE=TGGGGCTCGTATTCTAGTTCTT
PRIMER_LEFT_4=123,22
PRIMER_RIGHT_4=237,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=59.286
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_01369 Ps_contig_mira-and-tgicl-ass_franssen_12995
SEQUENCE=AGCTAACTAACTCACTATTTAACTGCAACTAACTGGGATAACTAACTAACTGGATCCAAACGAAAAAGCTAACAGGAAATTTGTTTCGAATTGTAACCGAATTTTTACTAACTAACAGAAGTTCAGTTCACTAAAAACTTCAT
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01370 Ps_contig_mira-and-tgicl-ass_franssen_13001
SEQUENCE=CTTTATACCTATAAAGTCATCTTATTCATTTCTTCTCTTAAATTATTTAATTGTAACTATATATATATTAATCCGTGGATATGTTTCAAATCCATATAGCACCAGCCTCCTTCAACATAGGCACTAACTCATTCCTCACATGAAGACTCATAATTTTCTTAGAACCACCTCTGAACTGCTGTCCTATGAAAACAGCAGGCACGCTTGGTTTGCACCCCATTTGAAGCAACTCACTTTCAATCTGCGACCCGTTTGTGATTTTATCAAGCTCATAAACAATAGGGTTGGCTCCAAAGCTACGTATCAGCGATGTTACAGAGTGGCTCAAACAACAAGTACTCTTGCTGAATATCACCACTGGCTTTTCTACCTACCATGCTTGTGACAGTGTCCATGTATAATATTACAAATTTATGATATATAATTTGTGTGTCCCGAYTCTGTTTT
TARGET=58,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01371 Ps_contig_mira-and-tgicl-ass_franssen_13017
SEQUENCE=GTTCGACKGTTTCCTTCAGGAAACTCTCATTTCCAACTGACCACTCTCTCTCTAACCCACACTCTCTATGATACTCCATCTCATCTCTCTACCAAACCTCGACCTACCTCCATCGTCTGGAAAATGATTCCAACGGTGGCTCCACCTCGAACCACCCTTCAATCCTATGTTTCCTAGGTTTTCGATTGAAGTTAAACTGGAACCTTCAATCAAACATAAAATCGCCCCTCTAACCATCGTAGGCCCTCACCAGAGAAGATGAAGATTCATCTTCTCCGACCACCCGCCACCGTCAAAACCCATATATCCCATAAACATCTGT
TARGET=44,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01372 Ps_contig_mira-and-tgicl-ass_franssen_13025
SEQUENCE=CCAAACTAAACCCTACATTTTCTTCATTCTTCAATTAAGGTACACTACTTTTCTATTTCTCTTTTTACTTCAATGTTTTTTTTGTTTGTGAATGACGATGATGATCCAGTAACGAAACCCTAGTGAGRGATAATAGATATTAATACTATGGAGGGTGGTGAGAGAATCGGAGAAGATATGGATCTCGTTCATTCTGAAAATGCTGCTGCTGCTGCCGCTGTTGGTGTTGGTTCCGTTTCTCAGGATCAAG
TARGET=201,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01373 Ps_contig_mira-and-tgicl-ass_franssen_13042
SEQUENCE=GTTAGATGGGTTACCCCACTTCATAGTCCAACATGTCTCTCTAATTTGCAGAAAAGGAATGGATGGAGTGAATGTTTATCTCATATCTTCTGAATCAAAGACCACACTATAATGGCGTGTAGATTAAGTCTATTCTCTCTCTCTCTCTCTATGTGTTTCAGAGAGACTCTATGTTTTTCTTTTTTCACACACTGCTATGCTAAATGGTTATGCGTTTAAAAGATCTCACTTTCACCCTAAAAAAGCTCGACACTTTTGTCT
TARGET=134,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1950
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.135150
PRIMER_LEFT_SEQUENCE=AGAAAAGGAATGGATGGAGTGA
PRIMER_RIGHT_SEQUENCE=CGCATAACCATTTAGCATAGCA
PRIMER_LEFT=49,22
PRIMER_RIGHT=213,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=60.135
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=0.1953
PRIMER_LEFT_1_PENALTY=0.060196
PRIMER_RIGHT_1_PENALTY=0.135150
PRIMER_LEFT_1_SEQUENCE=GGAATGGATGGAGTGAATGTTT
PRIMER_RIGHT_1_SEQUENCE=CGCATAACCATTTAGCATAGCA
PRIMER_LEFT_1=55,22
PRIMER_RIGHT_1=213,22
PRIMER_LEFT_1_TM=60.060
PRIMER_RIGHT_1_TM=60.135
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.2447
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.135150
PRIMER_LEFT_2_SEQUENCE=GGTTACCCCACTTCATAGTCCA
PRIMER_RIGHT_2_SEQUENCE=CGCATAACCATTTAGCATAGCA
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=213,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.135
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.2447
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.135150
PRIMER_LEFT_3_SEQUENCE=TGGGTTACCCCACTTCATAGTC
PRIMER_RIGHT_3_SEQUENCE=CGCATAACCATTTAGCATAGCA
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=213,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=60.135
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.3221
PRIMER_LEFT_4_PENALTY=0.186949
PRIMER_RIGHT_4_PENALTY=0.135150
PRIMER_LEFT_4_SEQUENCE=AAAGGAATGGATGGAGTGAATG
PRIMER_RIGHT_4_SEQUENCE=CGCATAACCATTTAGCATAGCA
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=213,22
PRIMER_LEFT_4_TM=60.187
PRIMER_RIGHT_4_TM=60.135
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_01374 Ps_contig_mira-and-tgicl-ass_franssen_13052
SEQUENCE=CTCTTGCGAAAATGAGCCACTGCCAAGCTCACATAACTGAGGGGCGCGAACAAGAAGTTTTTTCAACTGTTCCAGCGTTACGCTTTTGTTAACCTTCAAAGTCTTCAATGATTTGCACCTACCAACAAGTTTCTCTAGCGCGTCGATGTTTACTTCATTGGTTAGGTTGGCAAAGTTCAACACTTCCAATGATGTAAAGCTTTCTGGGAAGCAACTCAACCAGTTACCGCTTTTGTCTTCGATACCATTCTCTTGTATGTCAAGCTCAGTTAAGTTCTTGCAATTAGTAGCAACAGCAGCTAAACCATCAGTGCTAAATCCATCACAGCTCAAAAGAGAAAGCGCTTTAAAATTCGGAAACGAAAAAGCCAGAAACTCTAAACTCTCATCAGTAACAACCATTCTCTTAAGCCTTAACTCTTCAAGAAAAGGGTACTTTTCAGCGAAAACAACAAGCCATGGATGAATATCAGCACCCCAATTAGCAGGAACCAAGTTGAAATCAGAGAAACGTGGCTTGCCTTTCAATGTAACACTTCGAACATTCGGAAATCTTTGAGTCAAGATCTCTGGTGTAACGGAATAACAGTTACCTATGAACACATTCTTTCTCGACCATCTTTCAGCATCGAACCAGTCTTTGCAAACCAATGAAACCGAACTCTTGTCTTTGCGTGACTTCACCATGCTGAGAATTCTCTCCAGCACTTCATCTGGAAAGACTGAACTGGGATTCATGTTTTGTGGTTCCATCTTCCTCTTACACTTCATGATCAACACAACAACAACAAGAACAAAAAGAATTCAAAATCATAATAGATTTTTTTGTGAAATAATGGAAATATAGCAATGTATTAAAAGTGATTATAAGAAGTAAAAAGTTTGGTTTTATGTACTTTTGAAACCAAAAGTACTTGTTTTCATCCATGTTTATCT
TARGET=779,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01375 Ps_contig_mira-and-tgicl-ass_franssen_13068
SEQUENCE=CCGACAATACGAAACCTATTTTGAAGAACTCTTTTGTATTTTTGTAATTCATTCTTCTTCGCTCTTTCAATCGGTATTTGCTAGATCTCCATTTTTTCGTTTCTTCATCGAATCTTGGTGAACTAGAACCGTCAGATCAGGGAGTAGTGGAGAGTTGTGATTCGAAGTTTCATCATCGTCGCGATCACGTTCAAGCATCATCCCTGTCATCATCACCATAGATTGGAAGTGGAGGCGGTCAGATTGGAAGTAGAGAAAGAAGGGAGAGGATCCACGGTTCTTCATACGAGTTCTTCATTCCAGTTTTCATATATTCTTCACGTAACGGAAAGAAGGAATAAAATCGAAAGAAAACAACTAGCTTCAAAGAGAGTCCGATATATTCACTCCCCGATTCGTCATCATTGCAGATTTACCGGTAGCTCAACGAAGTGCTTCATCACTAATCAGCATCGGTCATCGCTACCTCCTCCTCCTCGTCGTCGGTGCAAATCGAAGAGCGAACAACAACGGAGCACAACGCTCGAAGAACGACGAACGCGCCACCGGAGCCACCGTGCCAGACTCGTCAGGTACCTTCGATTGATTTTTTTTCGTTCCTCTAGCAAATAAGATTGGTGATTTCCTACTCGACGACGACGAGAGGAGGATCCGGAAATTGTAGCGCCGCACTTAGGTTCTTAAAGAAGCTTCGTCTCCTCCMTGAACCATGGAAGATGTATC
TARGET=466,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01376 Ps_contig_mira-and-tgicl-ass_franssen_13068
SEQUENCE=CCGACAATACGAAACCTATTTTGAAGAACTCTTTTGTATTTTTGTAATTCATTCTTCTTCGCTCTTTCAATCGGTATTTGCTAGATCTCCATTTTTTCGTTTCTTCATCGAATCTTGGTGAACTAGAACCGTCAGATCAGGGAGTAGTGGAGAGTTGTGATTCGAAGTTTCATCATCGTCGCGATCACGTTCAAGCATCATCCCTGTCATCATCACCATAGATTGGAAGTGGAGGCGGTCAGATTGGAAGTAGAGAAAGAAGGGAGAGGATCCACGGTTCTTCATACGAGTTCTTCATTCCAGTTTTCATATATTCTTCACGTAACGGAAAGAAGGAATAAAATCGAAAGAAAACAACTAGCTTCAAAGAGAGTCCGATATATTCACTCCCCGATTCGTCATCATTGCAGATTTACCGGTAGCTCAACGAAGTGCTTCATCACTAATCAGCATCGGTCATCGCTACCTCCTCCTCCTCGTCGTCGGTGCAAATCGAAGAGCGAACAACAACGGAGCACAACGCTCGAAGAACGACGAACGCGCCACCGGAGCCACCGTGCCAGACTCGTCAGGTACCTTCGATTGATTTTTTTTCGTTCCTCTAGCAAATAAGATTGGTGATTTCCTACTCGACGACGACGAGAGGAGGATCCGGAAATTGTAGCGCCGCACTTAGGTTCTTAAAGAAGCTTCGTCTCCTCCMTGAACCATGGAAGATGTATC
TARGET=631,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01377 Ps_contig_mira-and-tgicl-ass_franssen_13096
SEQUENCE=TTCACCATAACCGTTTCTTCTTCTTCATCATCATGACAGGCGAAGGTGAAGAAGACCCAACATCAMAAAAAACCTTCTTCCTTCATCCCTAGGTGGCGGTGGCGGTATCGGCGAAGG
TARGET=15,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01378 Ps_contig_mira-and-tgicl-ass_franssen_13138
SEQUENCE=GGACGTTTGGTTTTTACCAAGTGATGATAGAAACNGAATAATAGTTTTGCAGTGTTGGTTCCTTGTTATGGAAGGGATTTTGGTTAGGGTTCAAGAGGATACTAAAACCAACTTGCTCAATGGTGTTGGGATTTTTTTGGATAGTTATTCTGATGAAAGCAAGAAATATGTGGAAAACTGGCTATCTTCTCTCCGAAACACTTGACGAACTAATCTCCATCATTTTGATCCTGTATCTTCATGACAAAATCCTCTGATATGATCTTTAGCCGACAAAAAATTGTATATTGTAAATAGTGTTAAAAAAAGAGCACTCTTATGCGATGTGATGTCTAATATGATTGGTAAATTTCTTGGATAATAATTGAAACCTATGAGGAGCAAACATTATAGTCAAGAATAAAGCAACTGTGGTGGTGGTGCTTTTGCTTTGAGAATGATGTGTAACTTCTGTTGTTCTTCTTCTCTTGCTTTGAGAATCGCGACAATGTGGCGGAATTGGGAGCAATGTGGATGATGCATGTTTGAAGTGTGGTGATTTTGTAGCTTGCAATTTGTTGATGTAAATATGAATGTAGATATTGGAATTTGATTTAATGCTTAATCTTGTTCTCAGAACTGTATATAAATTTTGATAATAACATCAGTTTATTCTGTATAGATGTATAWAWMTATGTA
TARGET=411,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01379 Ps_contig_mira-and-tgicl-ass_franssen_13157
SEQUENCE=AGAAAATGTATAARRWTWTTCTAAATCCATGGTGATATTATTATTGAATTGAATACACAACGCACAAGGATCAGGCCCCAAAGACCTTTTCACCTCTCCATTTTTCAAAGCCAATTAGGCCACTATGTAAAAGAGAGTTCACCTTCTTTTTTGAAAACCAATAAAAAAATTAAAAGTATATATATAAAAAAACTATATACACGTGTTTCCCATCACACGCGTTTGACACCAAATAATGGAGTCTTGTATAGTTAGGCCAAACCTCGTGGACCAAAAAAAGAGAAGTAAAAAAAATCACTCAAACACTAGTGATTGGCACGTGCCAAATCCGTCTTCACACGTACCATAGGAAAAGTCCATAGAAATGTAGTAGATGTTTTGTACTAGTATTGTTGAGACTTGAGAGTACCCATTCAAAGGAAAGTGAGTGGCGCCAAGAAAAGAAACTTCACTCATAAATCTCTTCCCATAACAAGTCAAAAACAGCTCAATTTTTCAGAGTTTTCATCCCCCATTGATGCATGCTCTGTTTTTTTTCTTCCATGTTTCTAGAGCACTGACCCACCATCTTTCATCTCATTCACCTATAATCAATGTAACCTTTGAATTCCTATCCCCGACATGGAATTCATCTATCTCTATTTTCTTCACTGTCACACACTCTGTTTCTTGTAGCAATAAATGTGTGTTCCAAAATCAACCTCCCCTAGGGAATCTCTGCAAACCCATTGAATGTGGATACATACCCTCAAAACATAAAAAAAG
TARGET=177,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01380 Ps_contig_mira-and-tgicl-ass_franssen_13159
SEQUENCE=GGAACGAATTGTGGCGCGTTTCATTCTTCATTCGGAGGGAAAAGAAAATAAATGGCTTCTTCAACTTCTTCTTCTTCTTGCCTCTTCCCCTCTTTCATCTCATCCAGCGCCAAACGCGTCCCAATCCAAATCTTCTTCAAATTAACAATCCCCAAAGCTTCTTCACAAGTTCACTGCCAGTGTCGTTCAAATTCCAATTCTTATTCAAATCAATCTCACGGAATCGAACGCCGTTGGGTTGAAGCATTCGATCAATCCCTCGCATCGSCGGAGAGATT
TARGET=65,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01381 Ps_contig_mira-and-tgicl-ass_franssen_13183
SEQUENCE=GCAATAGTCAAAGCATTAAAGGATTTATTCATAAGTCTCACAACTCGCAAACTCCACACTTAAGGATTCATTCAACCCAAAAGAAAAGTGACAGAAAAAATAAGATTAAGATTGTTACTTCAATACAACAACTCCCAACAACACAACACACACACACACGTTAGCAAGCAAGCAACACAACACTGGAACCTTCTAAATTCTATCAAATATCAATTATTCCTAATATTGATTGATATTCCTACCTCATTGATGGCAACACTTGTCCATACAAAGAACCTAATAATTACTAGATTATTACCATTGCAACATTACAGAGTGAGATGTTTGGCTAATTACTATTTGTTTAAGTTAAAGTTTCCAGCAATTAGCATCAAATGATAGCTTCAAAAATTTGTGAATACTTGAATTGAAGATCGAAAAAGGAGATGACAAGAAGCTAGAGTTGTGAGTGTTGCAAATCAAGTGCGGCAGAACGATACTTGCGATCTAGAGCTTCTGAAATAGGCGAAACCAATACTGAATCACGAGCTCTGGGTTCAATTAAAGTTGTGTTTCTTTGTTCACTCTGGGTTACTCACGAGCTAAAAACAAAATCTTTTTTAGAAGATGATTTAAAACCCTAAGCTGAAA
TARGET=146,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7992
PRIMER_LEFT_PENALTY=0.338748
PRIMER_RIGHT_PENALTY=0.460467
PRIMER_LEFT_SEQUENCE=CAACTCGCAAACTCCACACTTA
PRIMER_RIGHT_SEQUENCE=CTTTGTATGGACAAGTGTTGCC
PRIMER_LEFT=40,22
PRIMER_RIGHT=272,22
PRIMER_LEFT_TM=60.339
PRIMER_RIGHT_TM=59.540
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.8080
PRIMER_LEFT_1_PENALTY=0.347541
PRIMER_RIGHT_1_PENALTY=0.460467
PRIMER_LEFT_1_SEQUENCE=ACAACAACTCCCAACAACACAA
PRIMER_RIGHT_1_SEQUENCE=CTTTGTATGGACAAGTGTTGCC
PRIMER_LEFT_1=124,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=60.348
PRIMER_RIGHT_1_TM=59.540
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.8981
PRIMER_LEFT_2_PENALTY=0.338748
PRIMER_RIGHT_2_PENALTY=0.559375
PRIMER_LEFT_2_SEQUENCE=CAACTCGCAAACTCCACACTTA
PRIMER_RIGHT_2_SEQUENCE=GACAAGTGTTGCCATCAATGAG
PRIMER_LEFT_2=40,22
PRIMER_RIGHT_2=263,22
PRIMER_LEFT_2_TM=60.339
PRIMER_RIGHT_2_TM=60.559
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.9053
PRIMER_LEFT_3_PENALTY=0.338748
PRIMER_RIGHT_3_PENALTY=0.566538
PRIMER_LEFT_3_SEQUENCE=CAACTCGCAAACTCCACACTTA
PRIMER_RIGHT_3_SEQUENCE=TGCCATCAATGAGGTAGGAATA
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=254,22
PRIMER_LEFT_3_TM=60.339
PRIMER_RIGHT_3_TM=59.433
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=0.9069
PRIMER_LEFT_4_PENALTY=0.347541
PRIMER_RIGHT_4_PENALTY=0.559375
PRIMER_LEFT_4_SEQUENCE=ACAACAACTCCCAACAACACAA
PRIMER_RIGHT_4_SEQUENCE=GACAAGTGTTGCCATCAATGAG
PRIMER_LEFT_4=124,22
PRIMER_RIGHT_4=263,22
PRIMER_LEFT_4_TM=60.348
PRIMER_RIGHT_4_TM=60.559
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=140
=
PRIMER_SEQUENCE_ID=SSR_01382 Ps_contig_mira-and-tgicl-ass_franssen_13183
SEQUENCE=GCAATAGTCAAAGCATTAAAGGATTTATTCATAAGTCTCACAACTCGCAAACTCCACACTTAAGGATTCATTCAACCCAAAAGAAAAGTGACAGAAAAAATAAGATTAAGATTGTTACTTCAATACAACAACTCCCAACAACACAACACACACACACACGTTAGCAAGCAAGCAACACAACACTGGAACCTTCTAAATTCTATCAAATATCAATTATTCCTAATATTGATTGATATTCCTACCTCATTGATGGCAACACTTGTCCATACAAAGAACCTAATAATTACTAGATTATTACCATTGCAACATTACAGAGTGAGATGTTTGGCTAATTACTATTTGTTTAAGTTAAAGTTTCCAGCAATTAGCATCAAATGATAGCTTCAAAAATTTGTGAATACTTGAATTGAAGATCGAAAAAGGAGATGACAAGAAGCTAGAGTTGTGAGTGTTGCAAATCAAGTGCGGCAGAACGATACTTGCGATCTAGAGCTTCTGAAATAGGCGAAACCAATACTGAATCACGAGCTCTGGGTTCAATTAAAGTTGTGTTTCTTTGTTCACTCTGGGTTACTCACGAGCTAAAAACAAAATCTTTTTTAGAAGATGATTTAAAACCCTAAGCTGAAA
TARGET=163,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5341
PRIMER_LEFT_PENALTY=0.073623
PRIMER_RIGHT_PENALTY=0.460467
PRIMER_LEFT_SEQUENCE=ACACAACACACACACACACGTT
PRIMER_RIGHT_SEQUENCE=CTTTGTATGGACAAGTGTTGCC
PRIMER_LEFT=140,22
PRIMER_RIGHT=272,22
PRIMER_LEFT_TM=59.926
PRIMER_RIGHT_TM=59.540
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=0.6278
PRIMER_LEFT_1_PENALTY=0.167348
PRIMER_RIGHT_1_PENALTY=0.460467
PRIMER_LEFT_1_SEQUENCE=AACTCCCAACAACACAACACAC
PRIMER_RIGHT_1_SEQUENCE=CTTTGTATGGACAAGTGTTGCC
PRIMER_LEFT_1=129,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=59.833
PRIMER_RIGHT_1_TM=59.540
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=0.6330
PRIMER_LEFT_2_PENALTY=0.073623
PRIMER_RIGHT_2_PENALTY=0.559375
PRIMER_LEFT_2_SEQUENCE=ACACAACACACACACACACGTT
PRIMER_RIGHT_2_SEQUENCE=GACAAGTGTTGCCATCAATGAG
PRIMER_LEFT_2=140,22
PRIMER_RIGHT_2=263,22
PRIMER_LEFT_2_TM=59.926
PRIMER_RIGHT_2_TM=60.559
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=0.6402
PRIMER_LEFT_3_PENALTY=0.073623
PRIMER_RIGHT_3_PENALTY=0.566538
PRIMER_LEFT_3_SEQUENCE=ACACAACACACACACACACGTT
PRIMER_RIGHT_3_SEQUENCE=TGCCATCAATGAGGTAGGAATA
PRIMER_LEFT_3=140,22
PRIMER_RIGHT_3=254,22
PRIMER_LEFT_3_TM=59.926
PRIMER_RIGHT_3_TM=59.433
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=115
PRIMER_PAIR_PENALTY_4=0.7267
PRIMER_LEFT_4_PENALTY=0.167348
PRIMER_RIGHT_4_PENALTY=0.559375
PRIMER_LEFT_4_SEQUENCE=AACTCCCAACAACACAACACAC
PRIMER_RIGHT_4_SEQUENCE=GACAAGTGTTGCCATCAATGAG
PRIMER_LEFT_4=129,22
PRIMER_RIGHT_4=263,22
PRIMER_LEFT_4_TM=59.833
PRIMER_RIGHT_4_TM=60.559
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=135
=
PRIMER_SEQUENCE_ID=SSR_01383 Ps_contig_mira-and-tgicl-ass_franssen_13195
SEQUENCE=AAATTGGAAATTATATGTTTAGAAATGGTTGAATTGAAATGTAATGTATGGTTATAACATGATTTTGTTGGTTTATGGAAGTGTATATGGTGATGTCATGGTCTATGTATGGTATTAATGAATGAATGAAAACTTGGTTAGAAACTTGAATTT
TARGET=117,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01384 Ps_contig_mira-and-tgicl-ass_franssen_13228
SEQUENCE=AATAAAAGGGTAATTAACACCATTGTAAGACACAAATTCAAGATTAAAAGCTTTCAGAATTTCTGTGGTGACTAATCCTAGGGTTGAAGAAATGAAAACCCATATAAATGTTAATACTGCATCAGCAATTGCTAATAGTATTGCATTCGCCATTTTTCTTTCTTCAATTGAATCTCGTTCTACAATATCATATCAAAGAAAGTTATGATTTTTGGTTTTGGTGAGAATTGATAATGATGATGATGAAGAAACAAAGTGACAAAAACAGAAAAGGAAGAAAATGATYGTGAATTAACAAATCAGATTCAGTTC
TARGET=234,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01385 Ps_contig_mira-and-tgicl-ass_franssen_13306
SEQUENCE=TTCCATACCCCTAATAGGAATTTTTTCCTCCTAACAAATTATATGGAAGGAGGAACCACCCCTTAAGTTAACCCAAAATGGAAGAAAGGTGGTTTTTTTAAAAAAAAAGTACTAGAGCATAGAAAAAGCTCAGCATATGAAAGAGATCCATGTCAACACGAAAAAACTTAATAAAATATTCACAAAAGTTCTCAGTCCCACAAAACAATTCCATTTTCATTTCAACAAGATCGATCTCTCTCTCTCTCTCCATCGGTATCAACAAAAAAGAATCAATCTTTGAACTGTGTGAACTCGCTCACTCGTTCACTAACTAACTCACTAACTCGTGAATGGCGAAGAGCAACGAAGAACCAAATTCGAATCTGAATACTAACAAACCACCGTTGAAGAGAACAAAAACACTCGCACAGCAACCTTCTCTCAATCTCAGAGTCTCTATCGCCGCCGCCGACAATGGAATCGGAAATTCATCTTCATCTTCAACCAAAACCGATTTYGAACAACAACAGWGGAACTACCCTTCTTTTCTTGGCATTGGCTCCACCTCCAGRAAAAGAAGACCGCCACCTCCTCCCAAACCTTCCAACMTCACTCCGAACSTAAAACCTCCCGCCTCCGACTTCCAAACCAAACCTCACTCCGAACCTAAAACCTCTCCCTCTTCCTCTTCGCCACCTTCTCTCCCCATCGCCATCACCAAACAACAACAACAACAACACTCTATCTCCTCTCCAATTTTCTATCTCTTYGTTATCACATGTGTTATTTTTGTGCCTTATTCAGCTTTTCTACAATACAAGCTTGCCAAACTCAAGGATATGAAACTTCAACTTTGTTGTCAAATTGATTTTTGTTCTGGSAATGGAAAAACATCTTTACAAAAAGATGTTGTTGATGATGGTTCCTTTTCTTATTACATTCTGAATGCAGATAGTAGAACAATTTCTTTGTATATTGTGCTTTTCACACTTGTTTTACCTTTTATATTGTACAAATATATTGATTATCTTCCTCAAATGATTAATTTCTCGAGGAGAACAAATAGTAATAAAGAGGATGTACCATTAAAGAAGAGAGTTGCTTATATGGTAGATGTGTTTTTCTCCATATATCCTTATGCAAAGCTGCTTGCACTTCTTTTTGCAACTCTCTTTCTTATAGCATTTGGTGGTTTAGCGTTGTATGCGGTTACTGGCGGTAGCATGGCTGAAGCACTTTGGCATTCATGGACTTATGTAGCCGACGCAGGAAATCATGCTGAAACGGAAGGAATGGGCCAGAGAATCGTCTCTGTCTCAATTAGCGCAGGTGGCATGCTTATATTTGCCATGATGCTTGGGCTTGTTTCGGATGCTATATCGGAGAAGGTTGATTCACTTAKAAAAGGGAAGAGTGAAGTGATCGAAAGAAACCATGTACTCATCCTTGGGTGGAGTGACAAATTGGGCTCACTTTTGAAGCAGCTAGCAATAGCAAATAAGAGTGTCGGTGGTGGTGTTATTGTGGTGCTTGCTGAAAAGGAAAAGGAAGAAATGGAAATGGATATAGCCAAGCTAGAATTTGATTTCATGGGGACGTCTGTAATATGTAGAAGTGGCAGTCCACTTATACTGGCTGACTTAAAGAAGGTTTCAGTTTCAAAGGCACGTGCAATCATTGTTTTAGCTTCGGACGAAAATGCAGATCAGAGTGATGCACGTGCTTTAAGAGTTGTTCTTAGCTTAACTGGTGTAAAGGAGGGCTTAAGGGGACATGTTGTTGTGGAGATGAGTGACCTTGACAATGAACCCCTAGTGAAACTTGTTGGTGGGGAACTCATTGAAACAGTTGTTGCACATGATGTAATTGGACGTTTGATGATTCAATGTGCTCTGCAGCCTGGCCTTGCACAGATATGGGAGGACATTCTGGGATTTGAGAATGCTGAATTTTACATAAAAAGATGGCCTGAATTGGATGGTCTTCTTTTCAAAGACATATTAATTTCATTTCCTGATGCAATACCCTGTGGGGTTAAGGTTTCTGCAGATGGTGGGAAGATTGTCATAAATCCAGATGATAATTATGTTCTGAGAGATGGTGATGAAGTCCTTGTCATAGCTGAGGATGACGACACCTATGCTCCAGGCCCTCTCCCAGAGGTACGCAAGGGCTATTTTCCCAGGATACGTGACCCCCCTAAATATCCAGAGAAAATATTATTTTGTGGCTGGCGCCGTGACATTGATGATATGATCATGGTTTTAGAAGCATTCTTGGCCCCTGGTTCAGAACTCTGGATGTTCAACGAGGTTCCTGAAAAGCAAAGAGAGAGGAAACTTGCTGCTGGTGAACTTGATGTTTTTGGSCTAGAGAACATAAAGCTTGTCCACAGGGAGGGAAATGCTGTCATTAGGCGGCACCTGGAGAGTCTTCCATTGGAGACTTTTGATTCTATCCTTATTCTTGCAGATGAGTCAGTGGAGGACTCTGTTGCTCATTCCGACTCAAGATCCCTAGCTACCCTTCTGCTCATTCGTGATATACAGTCGAGACGTCTACCATACAGAGACACAAAGTCAACTTCTTTGAGGTTATCCGGGTTCTCTCACAACTCATGGATCCGCGAAATGCAGCAAGCTTCAGATAAATCAATTATAATTAGTGAAATTTTGGATTCTAGAACTAGAAATCTAGTTTCTGTGTCCAGAATAAGTGATTATGTATTATCTAATGAGCTGGTTAGCATGGCACTAGCTATGGTGGCTGAAGACAAGCAAATCAACCGTGTTCTTGAGGAATTATTTGCAGAGGAGGGGAACGAGATGTGTATTAAACCTGCAGAGTTCTATTTATTTGATCAAGAGGAGCTATGTTTTTATGATATAATGATTAGGGGTCGTACAAGAAAGGAAATCGTTATAGGATATCGCTTGGCCAGCCAAGAACGTGCTCTTATCAACCCTTCAGAAAAATCAATGACAAGAAAATGGTCACTTGATGATGTTTTCGTTGTTATTGCCTCAGGAGAATGAAAGAATAGTATAGTATTATTCTTGTTAGTTAGGTTATGTCTTCATCAGATTCATCATGCATGCATGCATATGATGGCCCTTCCTTTCCCTCTAAATTTAACTTGCCAAAATGTACTGACGGTGGCAGCTAGTTAACTGATGATAGCAGTTGGGAATTTGTAGACTCCTTAAAATTAGAAGGAACAATGGTTTTACCGGCTCGAAAATTCCTCATGATAATGGTTTATTGTGTAATTTAATGTGAATTGTTGTATCTGTAACAAAACTGTAACTTGTAAAATAATCTTTCAGAAATAGATGTAGGTGACTAGGGTCTAGGGACAAGTTCAAGTTGCGGAGGATAACAGATCTTTTGAAACTTTGGACTCAATGAGAGTGAGTAAGATGCTGTACTGATTCAAAGAGGTCTATATATAAAATTCACTTTTGTTTCTTTTTATWATT
TARGET=235,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01386 Ps_contig_mira-and-tgicl-ass_franssen_13306
SEQUENCE=TTCCATACCCCTAATAGGAATTTTTTCCTCCTAACAAATTATATGGAAGGAGGAACCACCCCTTAAGTTAACCCAAAATGGAAGAAAGGTGGTTTTTTTAAAAAAAAAGTACTAGAGCATAGAAAAAGCTCAGCATATGAAAGAGATCCATGTCAACACGAAAAAACTTAATAAAATATTCACAAAAGTTCTCAGTCCCACAAAACAATTCCATTTTCATTTCAACAAGATCGATCTCTCTCTCTCTCTCCATCGGTATCAACAAAAAAGAATCAATCTTTGAACTGTGTGAACTCGCTCACTCGTTCACTAACTAACTCACTAACTCGTGAATGGCGAAGAGCAACGAAGAACCAAATTCGAATCTGAATACTAACAAACCACCGTTGAAGAGAACAAAAACACTCGCACAGCAACCTTCTCTCAATCTCAGAGTCTCTATCGCCGCCGCCGACAATGGAATCGGAAATTCATCTTCATCTTCAACCAAAACCGATTTYGAACAACAACAGWGGAACTACCCTTCTTTTCTTGGCATTGGCTCCACCTCCAGRAAAAGAAGACCGCCACCTCCTCCCAAACCTTCCAACMTCACTCCGAACSTAAAACCTCCCGCCTCCGACTTCCAAACCAAACCTCACTCCGAACCTAAAACCTCTCCCTCTTCCTCTTCGCCACCTTCTCTCCCCATCGCCATCACCAAACAACAACAACAACAACACTCTATCTCCTCTCCAATTTTCTATCTCTTYGTTATCACATGTGTTATTTTTGTGCCTTATTCAGCTTTTCTACAATACAAGCTTGCCAAACTCAAGGATATGAAACTTCAACTTTGTTGTCAAATTGATTTTTGTTCTGGSAATGGAAAAACATCTTTACAAAAAGATGTTGTTGATGATGGTTCCTTTTCTTATTACATTCTGAATGCAGATAGTAGAACAATTTCTTTGTATATTGTGCTTTTCACACTTGTTTTACCTTTTATATTGTACAAATATATTGATTATCTTCCTCAAATGATTAATTTCTCGAGGAGAACAAATAGTAATAAAGAGGATGTACCATTAAAGAAGAGAGTTGCTTATATGGTAGATGTGTTTTTCTCCATATATCCTTATGCAAAGCTGCTTGCACTTCTTTTTGCAACTCTCTTTCTTATAGCATTTGGTGGTTTAGCGTTGTATGCGGTTACTGGCGGTAGCATGGCTGAAGCACTTTGGCATTCATGGACTTATGTAGCCGACGCAGGAAATCATGCTGAAACGGAAGGAATGGGCCAGAGAATCGTCTCTGTCTCAATTAGCGCAGGTGGCATGCTTATATTTGCCATGATGCTTGGGCTTGTTTCGGATGCTATATCGGAGAAGGTTGATTCACTTAKAAAAGGGAAGAGTGAAGTGATCGAAAGAAACCATGTACTCATCCTTGGGTGGAGTGACAAATTGGGCTCACTTTTGAAGCAGCTAGCAATAGCAAATAAGAGTGTCGGTGGTGGTGTTATTGTGGTGCTTGCTGAAAAGGAAAAGGAAGAAATGGAAATGGATATAGCCAAGCTAGAATTTGATTTCATGGGGACGTCTGTAATATGTAGAAGTGGCAGTCCACTTATACTGGCTGACTTAAAGAAGGTTTCAGTTTCAAAGGCACGTGCAATCATTGTTTTAGCTTCGGACGAAAATGCAGATCAGAGTGATGCACGTGCTTTAAGAGTTGTTCTTAGCTTAACTGGTGTAAAGGAGGGCTTAAGGGGACATGTTGTTGTGGAGATGAGTGACCTTGACAATGAACCCCTAGTGAAACTTGTTGGTGGGGAACTCATTGAAACAGTTGTTGCACATGATGTAATTGGACGTTTGATGATTCAATGTGCTCTGCAGCCTGGCCTTGCACAGATATGGGAGGACATTCTGGGATTTGAGAATGCTGAATTTTACATAAAAAGATGGCCTGAATTGGATGGTCTTCTTTTCAAAGACATATTAATTTCATTTCCTGATGCAATACCCTGTGGGGTTAAGGTTTCTGCAGATGGTGGGAAGATTGTCATAAATCCAGATGATAATTATGTTCTGAGAGATGGTGATGAAGTCCTTGTCATAGCTGAGGATGACGACACCTATGCTCCAGGCCCTCTCCCAGAGGTACGCAAGGGCTATTTTCCCAGGATACGTGACCCCCCTAAATATCCAGAGAAAATATTATTTTGTGGCTGGCGCCGTGACATTGATGATATGATCATGGTTTTAGAAGCATTCTTGGCCCCTGGTTCAGAACTCTGGATGTTCAACGAGGTTCCTGAAAAGCAAAGAGAGAGGAAACTTGCTGCTGGTGAACTTGATGTTTTTGGSCTAGAGAACATAAAGCTTGTCCACAGGGAGGGAAATGCTGTCATTAGGCGGCACCTGGAGAGTCTTCCATTGGAGACTTTTGATTCTATCCTTATTCTTGCAGATGAGTCAGTGGAGGACTCTGTTGCTCATTCCGACTCAAGATCCCTAGCTACCCTTCTGCTCATTCGTGATATACAGTCGAGACGTCTACCATACAGAGACACAAAGTCAACTTCTTTGAGGTTATCCGGGTTCTCTCACAACTCATGGATCCGCGAAATGCAGCAAGCTTCAGATAAATCAATTATAATTAGTGAAATTTTGGATTCTAGAACTAGAAATCTAGTTTCTGTGTCCAGAATAAGTGATTATGTATTATCTAATGAGCTGGTTAGCATGGCACTAGCTATGGTGGCTGAAGACAAGCAAATCAACCGTGTTCTTGAGGAATTATTTGCAGAGGAGGGGAACGAGATGTGTATTAAACCTGCAGAGTTCTATTTATTTGATCAAGAGGAGCTATGTTTTTATGATATAATGATTAGGGGTCGTACAAGAAAGGAAATCGTTATAGGATATCGCTTGGCCAGCCAAGAACGTGCTCTTATCAACCCTTCAGAAAAATCAATGACAAGAAAATGGTCACTTGATGATGTTTTCGTTGTTATTGCCTCAGGAGAATGAAAGAATAGTATAGTATTATTCTTGTTAGTTAGGTTATGTCTTCATCAGATTCATCATGCATGCATGCATATGATGGCCCTTCCTTTCCCTCTAAATTTAACTTGCCAAAATGTACTGACGGTGGCAGCTAGTTAACTGATGATAGCAGTTGGGAATTTGTAGACTCCTTAAAATTAGAAGGAACAATGGTTTTACCGGCTCGAAAATTCCTCATGATAATGGTTTATTGTGTAATTTAATGTGAATTGTTGTATCTGTAACAAAACTGTAACTTGTAAAATAATCTTTCAGAAATAGATGTAGGTGACTAGGGTCTAGGGACAAGTTCAAGTTGCGGAGGATAACAGATCTTTTGAAACTTTGGACTCAATGAGAGTGAGTAAGATGCTGTACTGATTCAAAGAGGTCTATATATAAAATTCACTTTTGTTTCTTTTTATWATT
TARGET=703,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01387 Ps_contig_mira-and-tgicl-ass_franssen_13306
SEQUENCE=TTCCATACCCCTAATAGGAATTTTTTCCTCCTAACAAATTATATGGAAGGAGGAACCACCCCTTAAGTTAACCCAAAATGGAAGAAAGGTGGTTTTTTTAAAAAAAAAGTACTAGAGCATAGAAAAAGCTCAGCATATGAAAGAGATCCATGTCAACACGAAAAAACTTAATAAAATATTCACAAAAGTTCTCAGTCCCACAAAACAATTCCATTTTCATTTCAACAAGATCGATCTCTCTCTCTCTCTCCATCGGTATCAACAAAAAAGAATCAATCTTTGAACTGTGTGAACTCGCTCACTCGTTCACTAACTAACTCACTAACTCGTGAATGGCGAAGAGCAACGAAGAACCAAATTCGAATCTGAATACTAACAAACCACCGTTGAAGAGAACAAAAACACTCGCACAGCAACCTTCTCTCAATCTCAGAGTCTCTATCGCCGCCGCCGACAATGGAATCGGAAATTCATCTTCATCTTCAACCAAAACCGATTTYGAACAACAACAGWGGAACTACCCTTCTTTTCTTGGCATTGGCTCCACCTCCAGRAAAAGAAGACCGCCACCTCCTCCCAAACCTTCCAACMTCACTCCGAACSTAAAACCTCCCGCCTCCGACTTCCAAACCAAACCTCACTCCGAACCTAAAACCTCTCCCTCTTCCTCTTCGCCACCTTCTCTCCCCATCGCCATCACCAAACAACAACAACAACAACACTCTATCTCCTCTCCAATTTTCTATCTCTTYGTTATCACATGTGTTATTTTTGTGCCTTATTCAGCTTTTCTACAATACAAGCTTGCCAAACTCAAGGATATGAAACTTCAACTTTGTTGTCAAATTGATTTTTGTTCTGGSAATGGAAAAACATCTTTACAAAAAGATGTTGTTGATGATGGTTCCTTTTCTTATTACATTCTGAATGCAGATAGTAGAACAATTTCTTTGTATATTGTGCTTTTCACACTTGTTTTACCTTTTATATTGTACAAATATATTGATTATCTTCCTCAAATGATTAATTTCTCGAGGAGAACAAATAGTAATAAAGAGGATGTACCATTAAAGAAGAGAGTTGCTTATATGGTAGATGTGTTTTTCTCCATATATCCTTATGCAAAGCTGCTTGCACTTCTTTTTGCAACTCTCTTTCTTATAGCATTTGGTGGTTTAGCGTTGTATGCGGTTACTGGCGGTAGCATGGCTGAAGCACTTTGGCATTCATGGACTTATGTAGCCGACGCAGGAAATCATGCTGAAACGGAAGGAATGGGCCAGAGAATCGTCTCTGTCTCAATTAGCGCAGGTGGCATGCTTATATTTGCCATGATGCTTGGGCTTGTTTCGGATGCTATATCGGAGAAGGTTGATTCACTTAKAAAAGGGAAGAGTGAAGTGATCGAAAGAAACCATGTACTCATCCTTGGGTGGAGTGACAAATTGGGCTCACTTTTGAAGCAGCTAGCAATAGCAAATAAGAGTGTCGGTGGTGGTGTTATTGTGGTGCTTGCTGAAAAGGAAAAGGAAGAAATGGAAATGGATATAGCCAAGCTAGAATTTGATTTCATGGGGACGTCTGTAATATGTAGAAGTGGCAGTCCACTTATACTGGCTGACTTAAAGAAGGTTTCAGTTTCAAAGGCACGTGCAATCATTGTTTTAGCTTCGGACGAAAATGCAGATCAGAGTGATGCACGTGCTTTAAGAGTTGTTCTTAGCTTAACTGGTGTAAAGGAGGGCTTAAGGGGACATGTTGTTGTGGAGATGAGTGACCTTGACAATGAACCCCTAGTGAAACTTGTTGGTGGGGAACTCATTGAAACAGTTGTTGCACATGATGTAATTGGACGTTTGATGATTCAATGTGCTCTGCAGCCTGGCCTTGCACAGATATGGGAGGACATTCTGGGATTTGAGAATGCTGAATTTTACATAAAAAGATGGCCTGAATTGGATGGTCTTCTTTTCAAAGACATATTAATTTCATTTCCTGATGCAATACCCTGTGGGGTTAAGGTTTCTGCAGATGGTGGGAAGATTGTCATAAATCCAGATGATAATTATGTTCTGAGAGATGGTGATGAAGTCCTTGTCATAGCTGAGGATGACGACACCTATGCTCCAGGCCCTCTCCCAGAGGTACGCAAGGGCTATTTTCCCAGGATACGTGACCCCCCTAAATATCCAGAGAAAATATTATTTTGTGGCTGGCGCCGTGACATTGATGATATGATCATGGTTTTAGAAGCATTCTTGGCCCCTGGTTCAGAACTCTGGATGTTCAACGAGGTTCCTGAAAAGCAAAGAGAGAGGAAACTTGCTGCTGGTGAACTTGATGTTTTTGGSCTAGAGAACATAAAGCTTGTCCACAGGGAGGGAAATGCTGTCATTAGGCGGCACCTGGAGAGTCTTCCATTGGAGACTTTTGATTCTATCCTTATTCTTGCAGATGAGTCAGTGGAGGACTCTGTTGCTCATTCCGACTCAAGATCCCTAGCTACCCTTCTGCTCATTCGTGATATACAGTCGAGACGTCTACCATACAGAGACACAAAGTCAACTTCTTTGAGGTTATCCGGGTTCTCTCACAACTCATGGATCCGCGAAATGCAGCAAGCTTCAGATAAATCAATTATAATTAGTGAAATTTTGGATTCTAGAACTAGAAATCTAGTTTCTGTGTCCAGAATAAGTGATTATGTATTATCTAATGAGCTGGTTAGCATGGCACTAGCTATGGTGGCTGAAGACAAGCAAATCAACCGTGTTCTTGAGGAATTATTTGCAGAGGAGGGGAACGAGATGTGTATTAAACCTGCAGAGTTCTATTTATTTGATCAAGAGGAGCTATGTTTTTATGATATAATGATTAGGGGTCGTACAAGAAAGGAAATCGTTATAGGATATCGCTTGGCCAGCCAAGAACGTGCTCTTATCAACCCTTCAGAAAAATCAATGACAAGAAAATGGTCACTTGATGATGTTTTCGTTGTTATTGCCTCAGGAGAATGAAAGAATAGTATAGTATTATTCTTGTTAGTTAGGTTATGTCTTCATCAGATTCATCATGCATGCATGCATATGATGGCCCTTCCTTTCCCTCTAAATTTAACTTGCCAAAATGTACTGACGGTGGCAGCTAGTTAACTGATGATAGCAGTTGGGAATTTGTAGACTCCTTAAAATTAGAAGGAACAATGGTTTTACCGGCTCGAAAATTCCTCATGATAATGGTTTATTGTGTAATTTAATGTGAATTGTTGTATCTGTAACAAAACTGTAACTTGTAAAATAATCTTTCAGAAATAGATGTAGGTGACTAGGGTCTAGGGACAAGTTCAAGTTGCGGAGGATAACAGATCTTTTGAAACTTTGGACTCAATGAGAGTGAGTAAGATGCTGTACTGATTCAAAGAGGTCTATATATAAAATTCACTTTTGTTTCTTTTTATWATT
TARGET=3072,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01388 Ps_contig_mira-and-tgicl-ass_franssen_13312
SEQUENCE=GCTTCTTCATCGAAGAACTGAACGACTCCTCGATTCTTCCTCTTGAGGCCGCCTGTCTTGTCATCGTCGTAACCGCGCTTGCGTTTTCCGTCGGAATCTTTCCCGGCGGGAGCTTTACCCTTTCCGTTTGCTTTGCCCTTGTTCGTCATTTCCGGCGAATTGAGACGAACGGGAATTGCATGCAGAGAAAGAGGGANAAATGAGGAGGGAGGGAGTGAGTGTGGAAAGTGTGAAGAGCGATGACTTC
TARGET=204,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0132
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=1.006271
PRIMER_LEFT_SEQUENCE=TCTTCATCGAAGAACTGAACGA
PRIMER_RIGHT_SEQUENCE=GCTCTTCACACTTTCCACACTC
PRIMER_LEFT=3,22
PRIMER_RIGHT=237,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=58.994
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=1.0648
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=1.057845
PRIMER_LEFT_1_SEQUENCE=TCTTCATCGAAGAACTGAACGA
PRIMER_RIGHT_1_SEQUENCE=AAGTCATCGCTCTTCACACTTTC
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=245,23
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=59.942
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=1.0648
PRIMER_LEFT_2_PENALTY=0.006945
PRIMER_RIGHT_2_PENALTY=1.057845
PRIMER_LEFT_2_SEQUENCE=TCTTCATCGAAGAACTGAACGA
PRIMER_RIGHT_2_SEQUENCE=GAAGTCATCGCTCTTCACACTTT
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=246,23
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=59.942
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=1.1986
PRIMER_LEFT_3_PENALTY=0.006945
PRIMER_RIGHT_3_PENALTY=1.191640
PRIMER_LEFT_3_SEQUENCE=TCTTCATCGAAGAACTGAACGA
PRIMER_RIGHT_3_SEQUENCE=GTCATCGCTCTTCACACTTTCC
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=61.192
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=1.3443
PRIMER_LEFT_4_PENALTY=0.338015
PRIMER_RIGHT_4_PENALTY=1.006271
PRIMER_LEFT_4_SEQUENCE=CTGTCTTGTCATCGTCGTAACC
PRIMER_RIGHT_4_SEQUENCE=GCTCTTCACACTTTCCACACTC
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=237,22
PRIMER_LEFT_4_TM=59.662
PRIMER_RIGHT_4_TM=58.994
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_01389 Ps_contig_mira-and-tgicl-ass_franssen_13313
SEQUENCE=CCGTTAGATATATTTAAATTAAACCAGCCCCCTCGCTTAGGGTATCTGCCCAAACTGTTGATTTTGTCTCAAACTTATCCTTGGAAGGTTGAGAAACTAAAGAATTTGATGTCGTTGAAACAGCATATGGTGAAGCATTGGGAGCAGGTGTTTGATGGGTATATGATGAACCTAAGGGAGCTGCATTCCCTTGTTGCCCAAAGAAGCCCTGGCTGTTGTTGGAAGGAGAACCGGTAGATCCTCCATGATGGGGAAAGTTTGTGATTTGTGCCGGTTGTTGTGCCGCTTGTGGTRTTGGAGCTTGGGGAGTCATGTGTGAAGGAAAACCTGTAGAGGATCCCTGTGGCAAGTAATTCCCACTGTTCATAGACCCATTATATCCATTTTGTGATTGAGAATACATGTTTGGAGCTCCTGGAGCAAGAGATGTATCCAGGGAGTACAATCCACCATAGGTATGACCACTTGATTGTGCAGATTGACCACCTTGAGAAGCATAAGGTGCCTGNATAGAATGGCCGAGTAGATAGATAGAAAAACTACC
TARGET=524,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01390 Ps_contig_mira-and-tgicl-ass_franssen_13314
SEQUENCE=AGTTTCTGAATTTATAGGAGAAGACAAAGTATGAAATGTTAAGGAAAGTGGAGAGTGAGATGAAATTCTAGATGAAGATGTTAGTGAATGTAGAGAAAAGAGTGGCATTTTCAAAGGTGGTGTCGGCGGCGGCGGCGGAGGGGTTGGTGGTGGAGGAGGAAGAGGAGGAGAAGTGGAAGTAGATGTTTTTGTACAATAAACAAAAAGTTTCAATCTCTTCATGTGTGTTTGAGATTGTAGTTTTGTGGAGAATTAGGTTTCTTTGTTTTGTACCAAAGAAAA
TARGET=124,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=5.1286
PRIMER_LEFT_PENALTY=1.256912
PRIMER_RIGHT_PENALTY=3.871638
PRIMER_LEFT_SEQUENCE=GGAAAGTGGAGAGTGAGATGAAA
PRIMER_RIGHT_SEQUENCE=CTTCCTCCTCCACCACCAAC
PRIMER_LEFT=42,23
PRIMER_RIGHT=161,20
PRIMER_LEFT_TM=59.743
PRIMER_RIGHT_TM=61.872
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=60.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=5.4352
PRIMER_LEFT_1_PENALTY=3.389252
PRIMER_RIGHT_1_PENALTY=2.045905
PRIMER_LEFT_1_SEQUENCE=GAAATGTTAAGGAAAGTGGAGAGTG
PRIMER_RIGHT_1_SEQUENCE=CTTCTCCTCCTCTTCCTCCTCC
PRIMER_LEFT_1=32,25
PRIMER_RIGHT_1=172,22
PRIMER_LEFT_1_TM=59.611
PRIMER_RIGHT_1_TM=62.046
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=59.091
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=5.5884
PRIMER_LEFT_2_PENALTY=3.389252
PRIMER_RIGHT_2_PENALTY=2.199179
PRIMER_LEFT_2_SEQUENCE=GAAATGTTAAGGAAAGTGGAGAGTG
PRIMER_RIGHT_2_SEQUENCE=TTCTCCTCCTCTTCCTCCTCC
PRIMER_LEFT_2=32,25
PRIMER_RIGHT_2=171,21
PRIMER_LEFT_2_TM=59.611
PRIMER_RIGHT_2_TM=61.199
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=57.143
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=5.7239
PRIMER_LEFT_3_PENALTY=5.353764
PRIMER_RIGHT_3_PENALTY=0.370176
PRIMER_LEFT_3_SEQUENCE=GGAGAGTGAGATGAAATTCTAGATG
PRIMER_RIGHT_3_SEQUENCE=CACTTCTCCTCCTCTTCCTCCT
PRIMER_LEFT_3=49,25
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=57.646
PRIMER_RIGHT_3_TM=60.370
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=5.8799
PRIMER_LEFT_4_PENALTY=2.008292
PRIMER_RIGHT_4_PENALTY=3.871638
PRIMER_LEFT_4_SEQUENCE=GGAAAGTGGAGAGTGAGATGAAAT
PRIMER_RIGHT_4_SEQUENCE=CTTCCTCCTCCACCACCAAC
PRIMER_LEFT_4=42,24
PRIMER_RIGHT_4=161,20
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=61.872
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_01391 Ps_contig_mira-and-tgicl-ass_franssen_13321
SEQUENCE=AACAGAGGTTGAGGCAAGGGGCCAAGTGGTAATTTTAATGGCAACCAAATCCCCGGAGATACCCGCCGAGACACCGTCTTCCGCCGCGGAACCATCTCAACCTCAAATTCAAACTCAACCTCCGACTTCTACTCCGCCTCTTCCTCCTACGAAACCACCACCACCACCACCGCCGACGCCCAAACTGGAAGCTCCCTTACCAGAGTCCAAACCCTCTACCGACACCGCCTTAATTCATATCCCATCTCACTCGAGATGGTTCTCAT
TARGET=154,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5408
PRIMER_LEFT_PENALTY=0.246779
PRIMER_RIGHT_PENALTY=0.294013
PRIMER_LEFT_SEQUENCE=GCCAAGTGGTAATTTTAATGGC
PRIMER_RIGHT_SEQUENCE=GGATATGAATTAAGGCGGTGTC
PRIMER_LEFT=20,22
PRIMER_RIGHT=241,22
PRIMER_LEFT_TM=59.753
PRIMER_RIGHT_TM=59.706
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.5751
PRIMER_LEFT_1_PENALTY=0.281044
PRIMER_RIGHT_1_PENALTY=0.294013
PRIMER_LEFT_1_SEQUENCE=TCAAACTCAACCTCCGACTTCT
PRIMER_RIGHT_1_SEQUENCE=GGATATGAATTAAGGCGGTGTC
PRIMER_LEFT_1=108,22
PRIMER_RIGHT_1=241,22
PRIMER_LEFT_1_TM=60.281
PRIMER_RIGHT_1_TM=59.706
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=0.5941
PRIMER_LEFT_2_PENALTY=0.246779
PRIMER_RIGHT_2_PENALTY=0.347318
PRIMER_LEFT_2_SEQUENCE=GCCAAGTGGTAATTTTAATGGC
PRIMER_RIGHT_2_SEQUENCE=TCTCGAGTGAGATGGGATATGA
PRIMER_LEFT_2=20,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=59.753
PRIMER_RIGHT_2_TM=59.653
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.6284
PRIMER_LEFT_3_PENALTY=0.281044
PRIMER_RIGHT_3_PENALTY=0.347318
PRIMER_LEFT_3_SEQUENCE=TCAAACTCAACCTCCGACTTCT
PRIMER_RIGHT_3_SEQUENCE=TCTCGAGTGAGATGGGATATGA
PRIMER_LEFT_3=108,22
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=60.281
PRIMER_RIGHT_3_TM=59.653
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=0.6700
PRIMER_LEFT_4_PENALTY=0.375941
PRIMER_RIGHT_4_PENALTY=0.294013
PRIMER_LEFT_4_SEQUENCE=GGCCAAGTGGTAATTTTAATGG
PRIMER_RIGHT_4_SEQUENCE=GGATATGAATTAAGGCGGTGTC
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=241,22
PRIMER_LEFT_4_TM=59.624
PRIMER_RIGHT_4_TM=59.706
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_01392 Ps_contig_mira-and-tgicl-ass_franssen_13336
SEQUENCE=CTCTCCTTCCAACAGTGGATGGTGGTGGAAAAATGGAGCTAAARATGCTGGTGGAAGAAGATGAAGTAGTGGAAGGATCTTTGGGACCAAAAAGATGATCGAAATTGGTAGGAGATGAGGAAGAACCAGCTCCTCCAACTTTCTTTTTGTTGTTCTTGTTCTCCATGTCTTTTGCTACAAAGTGTATGATTCACTACTACTATAATTAAGCTTTTTAAGTATTTTGAGTGCTTTTTATGAAAAGCAATTAACGAACAAAACAGGTTATGTAATAGAAAGAGTAAAGCTAAAGAACAAAACTAACGACTAAAAGATATTGTTGGATAAGGTTTGCATGGAAGAAGCAAAACAAAGTAAAGAGAGAGCAGAAGGCAGAGAAAAGGAGAGGAAGAACACGTGATCGATAGAAGATGTGTGTGTGTGTGTGTTC
TARGET=412,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01393 Ps_contig_mira-and-tgicl-ass_franssen_13381
SEQUENCE=TTCTTCAGTTCTTTCTGAAACTCCCAGTACAGGTTCAAGCAAAGATATTTAATTTGAAGTACCAAAACAGGCTTCTCTGTTATTCAACTCTATCCATTTTCATTCATTCACACGATTCTTCACAAGTATTTCTTATAGGACACTGGTCATTGTACTCTTACAGATATTGAATTCTTGTTAATCTGTTTAAATTAGGTGTATAAAGTATTCAGTTTTCTTCATTGTGTCTTTGGTTCTATTTGGAATTTTGAGTTCTGATTCTAGTTTCCTGTTTTACCAGAGTGCTTCTTTCTGTATATTCATATTAGTATCTGATATAAACAATAACTTCTACTCCTATTTTTTGTTTACAAG
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5058
PRIMER_LEFT_PENALTY=0.317768
PRIMER_RIGHT_PENALTY=1.187985
PRIMER_LEFT_SEQUENCE=AACTCCCAGTACAGGTTCAAGC
PRIMER_RIGHT_SEQUENCE=GAAGCACTCTGGTAAAACAGGAA
PRIMER_LEFT=18,22
PRIMER_RIGHT=287,23
PRIMER_LEFT_TM=59.682
PRIMER_RIGHT_TM=59.812
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=270
PRIMER_PAIR_PENALTY_1=1.5548
PRIMER_LEFT_1_PENALTY=0.366796
PRIMER_RIGHT_1_PENALTY=1.187985
PRIMER_LEFT_1_SEQUENCE=TGAAACTCCCAGTACAGGTTCA
PRIMER_RIGHT_1_SEQUENCE=GAAGCACTCTGGTAAAACAGGAA
PRIMER_LEFT_1=15,22
PRIMER_RIGHT_1=287,23
PRIMER_LEFT_1_TM=59.633
PRIMER_RIGHT_1_TM=59.812
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=1.7682
PRIMER_LEFT_2_PENALTY=0.580184
PRIMER_RIGHT_2_PENALTY=1.187985
PRIMER_LEFT_2_SEQUENCE=CCAGTACAGGTTCAAGCAAAGA
PRIMER_RIGHT_2_SEQUENCE=GAAGCACTCTGGTAAAACAGGAA
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=287,23
PRIMER_LEFT_2_TM=59.420
PRIMER_RIGHT_2_TM=59.812
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=1.8064
PRIMER_LEFT_3_PENALTY=0.317768
PRIMER_RIGHT_3_PENALTY=1.488657
PRIMER_LEFT_3_SEQUENCE=AACTCCCAGTACAGGTTCAAGC
PRIMER_RIGHT_3_SEQUENCE=GAAGCACTCTGGTAAAACAGGA
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=287,22
PRIMER_LEFT_3_TM=59.682
PRIMER_RIGHT_3_TM=58.511
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=1.8541
PRIMER_LEFT_4_PENALTY=0.317768
PRIMER_RIGHT_4_PENALTY=1.536322
PRIMER_LEFT_4_SEQUENCE=AACTCCCAGTACAGGTTCAAGC
PRIMER_RIGHT_4_SEQUENCE=AGAAGCACTCTGGTAAAACAGGA
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=288,23
PRIMER_LEFT_4_TM=59.682
PRIMER_RIGHT_4_TM=59.464
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_01394 Ps_contig_mira-and-tgicl-ass_franssen_13416
SEQUENCE=ATCAACGCAGAGTACGCGGGGGCAACAACTAATTTTTTACCTTCACACTACAATTTTCTTTAATTCCCCAAAAGGAACACACACACAAAAATAACCACTTTCTCTCTCTTCTCTCTCTATACATTGAAGCTTAGAATAAAGTGTTGAAAAAAAACAATAACATATTCAAGGTTCATTATTCATTATGGGCTTCAAGCTCAATGTGTTGCTGTTTGTGATACTTGCAACTTCTACTTATTTGCTCAACATGAAGCTTCTTGTTT
TARGET=77,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01395 Ps_contig_mira-and-tgicl-ass_franssen_13464
SEQUENCE=CAAGAAAGAAAGAACAATCGATGGATTTCTTTGTTTGGCGGTGGCTTCATTAAAGGGTCCTGAGTTTGCCACCATTGTCAATTCAGTGACATCGAAGAAAGTCGCGGCTTGATTACTTAATGTGGTTTTGGCAATATGAGAGCATTAAGGRAGGTGGCAATAATATTACTTGGTTTGGTCTTGATTCATCCTTTTATTATTTTCATTGCAATCAATAGGAGAATCATCCTCCCAGTTTTCTAAAGAACCTGTGTTTTATGGTATAGCATTTAGAGATACAAAGAATGTTAAGCAATAATGTTTMCTAADTTATTGGTTTGTTTTTTCTATAATTCTACTTTTACWATTAAGAAATCTTGGTCTTTATTTTTGGTCCTCCAGGGCGGCAATTGGAATCATGCATTGTACGAAAAGT
TARGET=2,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01396 Ps_contig_mira-and-tgicl-ass_franssen_13489
SEQUENCE=AAGCAGTGGTTATCAACGCAGAGTGGGGCATTGAGATGGCAAAGAGAAAGATCGCTCGAAAATATCATCGATCCACATCTTAAAGGAAACTATTGCCCGGAATCATTGTCTAAGTTCGGGGAGATAGCTGAAAAATGTCTCGCCGATGATGGTAAAAGCCGTCCCACAATGGGTGAGGTGTTATGGCACTTGGAATATGTGTTACAGCTACATGAAGCTTGGCTCAACATGGACAACAACAACAACAACAATAGCACAGAAAATTCATTCTCTAGAGATGAAGAAGCATGACCTTATATTATATATAGCTAAAATCCTCTACAGATTCTTTCTATTTGTTTTATTGAATTCAAGTTGTTTATTATTATCATTACTGTTTTTTAGCATCTGGTTCATTATGTATACATGGTAATGGATTTTCAAACATATGTCCAACAAAAATATGTTAATATTTGTGATATGCACTGCTTTTTGCATTGAGTTGCAACTGATTGTATTGTTACTGGGATCTTTCTTCAGAAAATGTTCATATCAATAGCTTCATAC
TARGET=233,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0565
PRIMER_LEFT_PENALTY=0.225403
PRIMER_RIGHT_PENALTY=0.831057
PRIMER_LEFT_SEQUENCE=TACATGAAGCTTGGCTCAACAT
PRIMER_RIGHT_SEQUENCE=CAACTCAATGCAAAAAGCAGTG
PRIMER_LEFT=208,22
PRIMER_RIGHT=483,22
PRIMER_LEFT_TM=59.775
PRIMER_RIGHT_TM=60.831
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=1.2715
PRIMER_LEFT_1_PENALTY=0.440473
PRIMER_RIGHT_1_PENALTY=0.831057
PRIMER_LEFT_1_SEQUENCE=CTACATGAAGCTTGGCTCAACA
PRIMER_RIGHT_1_SEQUENCE=CAACTCAATGCAAAAAGCAGTG
PRIMER_LEFT_1=207,22
PRIMER_RIGHT_1=483,22
PRIMER_LEFT_1_TM=60.440
PRIMER_RIGHT_1_TM=60.831
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=277
PRIMER_PAIR_PENALTY_2=1.8927
PRIMER_LEFT_2_PENALTY=0.225403
PRIMER_RIGHT_2_PENALTY=1.667347
PRIMER_LEFT_2_SEQUENCE=TACATGAAGCTTGGCTCAACAT
PRIMER_RIGHT_2_SEQUENCE=GCAACTCAATGCAAAAAGCAGT
PRIMER_LEFT_2=208,22
PRIMER_RIGHT_2=484,22
PRIMER_LEFT_2_TM=59.775
PRIMER_RIGHT_2_TM=61.667
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=2.0762
PRIMER_LEFT_3_PENALTY=1.245123
PRIMER_RIGHT_3_PENALTY=0.831057
PRIMER_LEFT_3_SEQUENCE=ACATGAAGCTTGGCTCAACAT
PRIMER_RIGHT_3_SEQUENCE=CAACTCAATGCAAAAAGCAGTG
PRIMER_LEFT_3=209,21
PRIMER_RIGHT_3=483,22
PRIMER_LEFT_3_TM=59.755
PRIMER_RIGHT_3_TM=60.831
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=2.1078
PRIMER_LEFT_4_PENALTY=0.440473
PRIMER_RIGHT_4_PENALTY=1.667347
PRIMER_LEFT_4_SEQUENCE=CTACATGAAGCTTGGCTCAACA
PRIMER_RIGHT_4_SEQUENCE=GCAACTCAATGCAAAAAGCAGT
PRIMER_LEFT_4=207,22
PRIMER_RIGHT_4=484,22
PRIMER_LEFT_4_TM=60.440
PRIMER_RIGHT_4_TM=61.667
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_01397 Ps_contig_mira-and-tgicl-ass_franssen_13538
SEQUENCE=CYTTGATGACACTGAAATTCTACCATATTTTCGTACTTCCTATTTCGTCATGTCATTTTAGTTTGTTTTATTTATTTATTTTGTTTATGTTATTTACTATGTYTCTCTTTGTTTTCAGGTTTTTACTTTGAATTGGAGCCCCGACCGAGAAAAGGAGTGAAAACGAGCTAAAAACCATAAAATTCAGCATTTTGCACTTGTGGCTCCCACTGTGGCTGGCGCCATGGGCCCAGCCATGACGTGTCCTAGCTCAACTTCCCTCAAATGCCACGTTCCCCACTAATTCCACTAAGGCGCCACAATTTGGCCTTATGGCGGGCGCCATGGACTTGTGGCGGGCGCCACAAGAAGGAAAGTTTTCCCTCCCAATTTCAAACTGAAGGGCATCCTTGTCATTTCCATTATTTTACTTGCCTATAAATAGAGACTCGAATTCATTTGTTTAATCATCCAAACTTAGAGCAGAGGCAAACTTAGAGCAAACTTAGTTTCACTTAGGCATATATTCAGTTATTTTAAAATGGTAATCGCTTCGCAGTTGGGGTNTTAACCACTAATTGTAGTAATCGTAGTTTG
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01398 Ps_contig_mira-and-tgicl-ass_franssen_13553
SEQUENCE=TCGGGGATCACTTTTCTTTCTTTCATTCTATCATTTCGTTGGCCAAATCTCTTTCACACTCAAAGATGACTCAGAAAAACAGCATGTACATCGACACCCCAGAAGCTTTTATCGATGGTGGCAAAAACTTTGACGATGATGGACGAGACAAAAGACTGGTACATGGGTGACTGCAAGTGCACACATCATAACAGCAGTGATAGGTTCAGGTATTTTANCACTTGCAGT
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01399 Ps_contig_mira-and-tgicl-ass_franssen_13560
SEQUENCE=CGCTGTTATTGTAGCTTTAATTGCAAATTTAGGGTTTGCCTTTGAATGTTATTTATAAATGTTTTTGTGTTTGTTTTGTTTGTTTGAGGAGAGTTCTATTTAGGTTTTCTATGTTAGGGCTTCCGATTTGGGGTTTTGGTTGGTAATTTTGGTTGTTTTT
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0099
PRIMER_LEFT_PENALTY=1.921134
PRIMER_RIGHT_PENALTY=0.088755
PRIMER_LEFT_SEQUENCE=GCAAATTTAGGGTTTGCCTTTG
PRIMER_RIGHT_SEQUENCE=TCGGAAGCCCTAACATAGAAAA
PRIMER_LEFT=22,22
PRIMER_RIGHT=125,22
PRIMER_LEFT_TM=61.921
PRIMER_RIGHT_TM=60.089
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=2.7766
PRIMER_LEFT_1_PENALTY=1.921134
PRIMER_RIGHT_1_PENALTY=0.855463
PRIMER_LEFT_1_SEQUENCE=GCAAATTTAGGGTTTGCCTTTG
PRIMER_RIGHT_1_SEQUENCE=ATCGGAAGCCCTAACATAGAAA
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=126,22
PRIMER_LEFT_1_TM=61.921
PRIMER_RIGHT_1_TM=59.145
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=2.7766
PRIMER_LEFT_2_PENALTY=1.921134
PRIMER_RIGHT_2_PENALTY=0.855463
PRIMER_LEFT_2_SEQUENCE=GCAAATTTAGGGTTTGCCTTTG
PRIMER_RIGHT_2_SEQUENCE=AATCGGAAGCCCTAACATAGAA
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=127,22
PRIMER_LEFT_2_TM=61.921
PRIMER_RIGHT_2_TM=59.145
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=106
PRIMER_PAIR_PENALTY_3=3.4627
PRIMER_LEFT_3_PENALTY=3.446387
PRIMER_RIGHT_3_PENALTY=0.016290
PRIMER_LEFT_3_SEQUENCE=AGGGTTTGCCTTTGAATGTT
PRIMER_RIGHT_3_SEQUENCE=CAAATCGGAAGCCCTAACATAG
PRIMER_LEFT_3=30,20
PRIMER_RIGHT_3=129,22
PRIMER_LEFT_3_TM=58.554
PRIMER_RIGHT_3_TM=59.984
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=3.5171
PRIMER_LEFT_4_PENALTY=2.056850
PRIMER_RIGHT_4_PENALTY=1.460253
PRIMER_LEFT_4_SEQUENCE=TTGCAAATTTAGGGTTTGCC
PRIMER_RIGHT_4_SEQUENCE=CAAAATTACCAACCAAAACCCC
PRIMER_LEFT_4=20,20
PRIMER_RIGHT_4=150,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=61.460
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=10.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=131
=
PRIMER_SEQUENCE_ID=SSR_01400 Ps_contig_mira-and-tgicl-ass_franssen_13571
SEQUENCE=AGGAAAGAATGGATCTCCGATCTTCTTAGATCCACCTGCGTACTAGCCTGGGTCGAAAATGAGCTGGAGTTTCTCCGAAGGAATTCATCGCATTGTGCGGCCAAGAAGACTCCGACGAAGCTCGCACAAGTCCGTTGCATGTTCAGATCCGTATAACGCTCCTTGTCTCCTTTAGATTCCATGTCTTACCTCACGAATTCATGTACTCTCCAATGAAGAATCAAGAACAACAATCGGATTCGTCGGATACTCTCAACATTGAATCGACATCGAGACCGAGACTGAATTAACTTCCTTTCTTTTTCTTGTGCGCGAATTGAAAGAAATTGAAGAGAGAGTGGTTACCTACCGGAATCGATTTGTTGACGGTGCGGTTGGTTGTTCGTTCTGTGAGTTCCTGCAGCATCGGCTAAACCGAGAGCGAAGAGGGAGAGCGTACTCGTGGCGCAATGGAGCTTCGAAGATGGAAAGCAGAATCGAAGCTGTGGATTCACCGGTTCAGATGATTGAAGAGTGATATTGGATTGAAGGTGATGAAATGGATTGAAGGTTGGTGTGGTGAAGGTTTTGTGAATGGAGGTTGAAGTGGCGGTTACCGGTTTTTGTGAGTTTGTTACGTTTGTTGAAGGAATTGAAGAGAGGAAGAAGAAGCCGGAAGCCGAGAAGTGAAGATTGAAGAAGAAACCTGATTGATGCGTGGGTTCCCTTTATAGAGCCCTGTTTTTGCAGATTTCTCTGTTAGAGTTTGTTAGTTAGTTATGAATTTTCTGTTAT
TARGET=748,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01401 Ps_contig_mira-and-tgicl-ass_franssen_13576
SEQUENCE=CTGTTTACATTAGTGTCTGTGTCACCCAAAGAATATGTTTCTTGTTGTTGTTGTGATGGTTGATATTTATGTTGATAAAAATGTTGATATTGTTGTCGGTGCTGAGGAGAATGTGAATGGTGATAGTGTTGGAGAATAGGGTGATGAATGTTGTTTGGTGCTAAGGTAAGTTGAGGGGTGGAGAGAATGGAGGAGTGAGAATCATCAATGGTGAGTTGTTGTTGTTGGGGATGGTAAAGGGTATTATTGGGAATTTCATTTTCATAGTAAGGCTGCATAAGCAATGCTATGATAAAGGAGGCAGGAAAGAATGGAAGTACTAAATAACACCTTTGTCAGGGAATAGAACTAGTGGTCTAAAAACGTACCCCGACGTACTCTGCGTTGTAA
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3646
PRIMER_LEFT_PENALTY=0.335679
PRIMER_RIGHT_PENALTY=0.028939
PRIMER_LEFT_SEQUENCE=AGTGTCTGTGTCACCCAAAGAA
PRIMER_RIGHT_SEQUENCE=TAGCATTGCTTATGCAGCCTTA
PRIMER_LEFT=11,22
PRIMER_RIGHT=288,22
PRIMER_LEFT_TM=59.664
PRIMER_RIGHT_TM=60.029
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=278
PRIMER_PAIR_PENALTY_1=0.3680
PRIMER_LEFT_1_PENALTY=0.335679
PRIMER_RIGHT_1_PENALTY=0.032347
PRIMER_LEFT_1_SEQUENCE=AGTGTCTGTGTCACCCAAAGAA
PRIMER_RIGHT_1_SEQUENCE=CCTTAGCACCAAACAACATTCA
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=165,22
PRIMER_LEFT_1_TM=59.664
PRIMER_RIGHT_1_TM=60.032
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=155
PRIMER_PAIR_PENALTY_2=0.4312
PRIMER_LEFT_2_PENALTY=0.335679
PRIMER_RIGHT_2_PENALTY=0.095523
PRIMER_LEFT_2_SEQUENCE=AGTGTCTGTGTCACCCAAAGAA
PRIMER_RIGHT_2_SEQUENCE=AGCACCAAACAACATTCATCAC
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=161,22
PRIMER_LEFT_2_TM=59.664
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=151
PRIMER_PAIR_PENALTY_3=0.4468
PRIMER_LEFT_3_PENALTY=0.335679
PRIMER_RIGHT_3_PENALTY=0.111077
PRIMER_LEFT_3_SEQUENCE=AGTGTCTGTGTCACCCAAAGAA
PRIMER_RIGHT_3_SEQUENCE=TCACCATTCACATTCTCCTCAG
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=122,22
PRIMER_LEFT_3_TM=59.664
PRIMER_RIGHT_3_TM=60.111
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=112
PRIMER_PAIR_PENALTY_4=0.5155
PRIMER_LEFT_4_PENALTY=0.335679
PRIMER_RIGHT_4_PENALTY=0.179801
PRIMER_LEFT_4_SEQUENCE=AGTGTCTGTGTCACCCAAAGAA
PRIMER_RIGHT_4_SEQUENCE=AATACCCTTTACCATCCCCAAC
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=244,22
PRIMER_LEFT_4_TM=59.664
PRIMER_RIGHT_4_TM=60.180
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_01402 Ps_contig_mira-and-tgicl-ass_franssen_13576
SEQUENCE=CTGTTTACATTAGTGTCTGTGTCACCCAAAGAATATGTTTCTTGTTGTTGTTGTGATGGTTGATATTTATGTTGATAAAAATGTTGATATTGTTGTCGGTGCTGAGGAGAATGTGAATGGTGATAGTGTTGGAGAATAGGGTGATGAATGTTGTTTGGTGCTAAGGTAAGTTGAGGGGTGGAGAGAATGGAGGAGTGAGAATCATCAATGGTGAGTTGTTGTTGTTGGGGATGGTAAAGGGTATTATTGGGAATTTCATTTTCATAGTAAGGCTGCATAAGCAATGCTATGATAAAGGAGGCAGGAAAGAATGGAAGTACTAAATAACACCTTTGTCAGGGAATAGAACTAGTGGTCTAAAAACGTACCCCGACGTACTCTGCGTTGTAA
TARGET=215,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1245
PRIMER_LEFT_PENALTY=0.095523
PRIMER_RIGHT_PENALTY=0.028939
PRIMER_LEFT_SEQUENCE=GTGATGAATGTTGTTTGGTGCT
PRIMER_RIGHT_SEQUENCE=TAGCATTGCTTATGCAGCCTTA
PRIMER_LEFT=140,22
PRIMER_RIGHT=288,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=60.029
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=0.1400
PRIMER_LEFT_1_PENALTY=0.111077
PRIMER_RIGHT_1_PENALTY=0.028939
PRIMER_LEFT_1_SEQUENCE=CTGAGGAGAATGTGAATGGTGA
PRIMER_RIGHT_1_SEQUENCE=TAGCATTGCTTATGCAGCCTTA
PRIMER_LEFT_1=101,22
PRIMER_RIGHT_1=288,22
PRIMER_LEFT_1_TM=60.111
PRIMER_RIGHT_1_TM=60.029
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=188
PRIMER_PAIR_PENALTY_2=0.2043
PRIMER_LEFT_2_PENALTY=0.032347
PRIMER_RIGHT_2_PENALTY=0.171921
PRIMER_LEFT_2_SEQUENCE=TGAATGTTGTTTGGTGCTAAGG
PRIMER_RIGHT_2_SEQUENCE=GTACGTCGGGGTACGTTTTTAG
PRIMER_LEFT_2=144,22
PRIMER_RIGHT_2=377,22
PRIMER_LEFT_2_TM=60.032
PRIMER_RIGHT_2_TM=59.828
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=234
PRIMER_PAIR_PENALTY_3=0.2043
PRIMER_LEFT_3_PENALTY=0.032347
PRIMER_RIGHT_3_PENALTY=0.171921
PRIMER_LEFT_3_SEQUENCE=TGAATGTTGTTTGGTGCTAAGG
PRIMER_RIGHT_3_SEQUENCE=AGTACGTCGGGGTACGTTTTTA
PRIMER_LEFT_3=144,22
PRIMER_RIGHT_3=378,22
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=59.828
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.2116
PRIMER_LEFT_4_PENALTY=0.182682
PRIMER_RIGHT_4_PENALTY=0.028939
PRIMER_LEFT_4_SEQUENCE=GGAGAGAATGGAGGAGTGAGAA
PRIMER_RIGHT_4_SEQUENCE=TAGCATTGCTTATGCAGCCTTA
PRIMER_LEFT_4=179,22
PRIMER_RIGHT_4=288,22
PRIMER_LEFT_4_TM=59.817
PRIMER_RIGHT_4_TM=60.029
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_01403 Ps_contig_mira-and-tgicl-ass_franssen_13586
SEQUENCE=GGTGGATCTATAAGAGAACGTTCACTTCACGKCKKKGAGTCTCTTCTAGGGTTAAAGCAGCGCAGCTAGAAAAAAAAGGGTTTTGTGAAAAAAATTTGAAATCATGAGTTCTAGGTTATCTGGGAAGGTGAAGTGGTTCAACGATCAGAAGGGGTTTGGATTCATTACCCCTGATGATGGTACCGAGGAGCTTTTCGTTCACCAATCTCAGATCCAGACTGATGGTTTCAGGAGTCTTGCTGAAGGTGAATCTGTCGAGTATCAGGTTGAATCTGATTCCGATGGACGATCCAAGGCTGTTCAGGTGACTGGTCCTGATGGTGCGTCTGTGCAGGGAAGCAAGCGTGGTGGAGGAGGKGGTTATGGAGGCGGTGGTGGTGGTTATGGAGGCGGTGGTGGCTACAACAGTGGCGGTGGAGGTGGTTATGGTGGAAGCAGAGGAGGTGGCGGATACGGTGGCGGTGGAAGCTGCTACAGCTGTGGAGAATCTGGTCATTTTGCGAGAGATTGCCCAACCAGTACCCGTTAGATCT
TARGET=371,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01404 Ps_contig_mira-and-tgicl-ass_franssen_13589
SEQUENCE=AGGAGTTTGAGAGAGGCGAGGGTTTGTGGGTTCTTTTTCTCAGGAGAAAAATTGTGAGGTGAGGGAAGAGAGATTGATTTGTGTGTTTTTCGTGACATTGTGAGTGAGGTCAGAGTGAGAGGATGAAGGTGAGTGGGTAGCGATTTTTTTTAGAGAGTTTTTTTGAGTGAGTGATTCAGAGTGAGTCCGTTGTGAGAGTTTTCGTGAGAATGAATAGTGAGTGAGTGAAATAATTTGTTTTTTTCAGCTGAGAGTGAGAGAGGKTGGAGTAGTTTTTTAGGGAGAAACGGTATTTTTGTTGGTGGTGAGGGGGATGGAGGTATTTTGTTTGTGTTGAGAGACGTTCGGTCTTCTTCCAGAGTAGTTTTCGTCCCCGCGTTA
TARGET=216,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01405 Ps_contig_mira-and-tgicl-ass_franssen_13594
SEQUENCE=CCTCCCGGTTCATTTTCCGATGCTTCTCCTCCTGGTATTTAACCTTATTATGGTTAGGAAGGAAGGTAGATTTCATCTGAACTACAATCCCTTTCCCCTATAGATTTTTTCTCTTCCACCAACTTTTCAAGTTGGATCTCATAGCATCATACCGAATTTCAGTTTTGGTTTCTAGTTTCGATTTCGAGTATGATGCCTTTTTCCCAAGTTACTATTTTTGGGTAAAAACTTTTACTTAGGGATCTTTATGTACTGCTTTATTTATTTAATCATTGCCTTTCTCCATGCCAACAACCCATCGATGGTAACAAAGGCATGCAGTAGTTAAATAATTTCATTTTATTTTAGTAATGATTTAGATTCCTGATTCTTTTTCCTG
TARGET=257,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5146
PRIMER_LEFT_PENALTY=0.055352
PRIMER_RIGHT_PENALTY=0.459263
PRIMER_LEFT_SEQUENCE=TGGATCTCATAGCATCATACCG
PRIMER_RIGHT_SEQUENCE=TACTGCATGCCTTTGTTACCAT
PRIMER_LEFT=132,22
PRIMER_RIGHT=322,22
PRIMER_LEFT_TM=59.945
PRIMER_RIGHT_TM=59.541
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.7714
PRIMER_LEFT_1_PENALTY=0.312177
PRIMER_RIGHT_1_PENALTY=0.459263
PRIMER_LEFT_1_SEQUENCE=GAGTATGATGCCTTTTTCCCAA
PRIMER_RIGHT_1_SEQUENCE=TACTGCATGCCTTTGTTACCAT
PRIMER_LEFT_1=185,22
PRIMER_RIGHT_1=322,22
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=59.541
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=0.8032
PRIMER_LEFT_2_PENALTY=0.343977
PRIMER_RIGHT_2_PENALTY=0.459263
PRIMER_LEFT_2_SEQUENCE=TGATGCCTTTTTCCCAAGTTAC
PRIMER_RIGHT_2_SEQUENCE=TACTGCATGCCTTTGTTACCAT
PRIMER_LEFT_2=190,22
PRIMER_RIGHT_2=322,22
PRIMER_LEFT_2_TM=60.344
PRIMER_RIGHT_2_TM=59.541
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=133
PRIMER_PAIR_PENALTY_3=0.8988
PRIMER_LEFT_3_PENALTY=0.439553
PRIMER_RIGHT_3_PENALTY=0.459263
PRIMER_LEFT_3_SEQUENCE=TTCGAGTATGATGCCTTTTTCC
PRIMER_RIGHT_3_SEQUENCE=TACTGCATGCCTTTGTTACCAT
PRIMER_LEFT_3=182,22
PRIMER_RIGHT_3=322,22
PRIMER_LEFT_3_TM=60.440
PRIMER_RIGHT_3_TM=59.541
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=141
PRIMER_PAIR_PENALTY_4=0.9182
PRIMER_LEFT_4_PENALTY=0.458951
PRIMER_RIGHT_4_PENALTY=0.459263
PRIMER_LEFT_4_SEQUENCE=CGATTTCGAGTATGATGCCTTT
PRIMER_RIGHT_4_SEQUENCE=TACTGCATGCCTTTGTTACCAT
PRIMER_LEFT_4=178,22
PRIMER_RIGHT_4=322,22
PRIMER_LEFT_4_TM=60.459
PRIMER_RIGHT_4_TM=59.541
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_01406 Ps_contig_mira-and-tgicl-ass_franssen_13620
SEQUENCE=GTTCGGGGKAGTTCGGACCACAAACAACAACTTCATCTCTTCTTTACAACTACCACTCCCTCAACTCTCTCTCTATAACAAGAAAAAACTACTCTCTCTCTAGAAGAAGAAGACAAAGAAGAGAGCATAGAGAATTCGGCCATGGATACGGCATCGCNGCTCGTCTACCGTGGAATCGACTCCCCTTTACCTTTCCGTTCCTCCTCACTTCCCCACCCGTCCAACCAAAAACCTCTCTCTCCGTCGCCGTTCAACCGTGTCTCCGCTGTCCG
TARGET=65,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01407 Ps_contig_mira-and-tgicl-ass_franssen_13620
SEQUENCE=GTTCGGGGKAGTTCGGACCACAAACAACAACTTCATCTCTTCTTTACAACTACCACTCCCTCAACTCTCTCTCTATAACAAGAAAAAACTACTCTCTCTCTAGAAGAAGAAGACAAAGAAGAGAGCATAGAGAATTCGGCCATGGATACGGCATCGCNGCTCGTCTACCGTGGAATCGACTCCCCTTTACCTTTCCGTTCCTCCTCACTTCCCCACCCGTCCAACCAAAAACCTCTCTCTCCGTCGCCGTTCAACCGTGTCTCCGCTGTCCG
TARGET=92,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01408 Ps_contig_mira-and-tgicl-ass_franssen_13620
SEQUENCE=GTTCGGGGKAGTTCGGACCACAAACAACAACTTCATCTCTTCTTTACAACTACCACTCCCTCAACTCTCTCTCTATAACAAGAAAAAACTACTCTCTCTCTAGAAGAAGAAGACAAAGAAGAGAGCATAGAGAATTCGGCCATGGATACGGCATCGCNGCTCGTCTACCGTGGAATCGACTCCCCTTTACCTTTCCGTTCCTCCTCACTTCCCCACCCGTCCAACCAAAAACCTCTCTCTCCGTCGCCGTTCAACCGTGTCTCCGCTGTCCG
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01409 Ps_contig_mira-and-tgicl-ass_franssen_13654
SEQUENCE=GAGTTCCATTTAATTGGGCATTGTTGTCTTCGCGAGTTCTAAGAATCCACTCTCATCCCTGTCTTTGAAGTGGGAAGCAGAACAACCCCTTACAACTTGGAAATTCCAAACTGAACTCCGATACAAACCATAAACAACACTTCACAGAATCGATTTCGTCCTATATACCTTACTTTATCATCATGTATTTGTTGACAGCGAAACCCTCCTTCAGACCTCAATTGACATTATCATCTTCTTCTTCTTCAACTTCTACTTCGTTATCTTCAATTTCTCGATTTTCCGTCCCAACTCGTTCACCTTCATCAATCACCTTTCCGAGAAATTCAAACCATAATGGAAGACTTAGTTTCAGAGTTGAATCATCCAAAAAATGACTCTCCCAATTCCGCTAGTGCCGGCGACT
TARGET=234,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0590
PRIMER_LEFT_PENALTY=0.039178
PRIMER_RIGHT_PENALTY=0.019805
PRIMER_LEFT_SEQUENCE=TGTATTTGTTGACAGCGAAACC
PRIMER_RIGHT_SEQUENCE=GTTTGAATTTCTCGGAAAGGTG
PRIMER_LEFT=183,22
PRIMER_RIGHT=331,22
PRIMER_LEFT_TM=60.039
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=0.0941
PRIMER_LEFT_1_PENALTY=0.039178
PRIMER_RIGHT_1_PENALTY=0.054900
PRIMER_LEFT_1_SEQUENCE=TGTATTTGTTGACAGCGAAACC
PRIMER_RIGHT_1_SEQUENCE=GAATTTCTCGGAAAGGTGATTG
PRIMER_LEFT_1=183,22
PRIMER_RIGHT_1=327,22
PRIMER_LEFT_1_TM=60.039
PRIMER_RIGHT_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=0.3122
PRIMER_LEFT_2_PENALTY=0.292414
PRIMER_RIGHT_2_PENALTY=0.019805
PRIMER_LEFT_2_SEQUENCE=TCACAGAATCGATTTCGTCCTA
PRIMER_RIGHT_2_SEQUENCE=GTTTGAATTTCTCGGAAAGGTG
PRIMER_LEFT_2=141,22
PRIMER_RIGHT_2=331,22
PRIMER_LEFT_2_TM=59.708
PRIMER_RIGHT_2_TM=59.980
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.3473
PRIMER_LEFT_3_PENALTY=0.292414
PRIMER_RIGHT_3_PENALTY=0.054900
PRIMER_LEFT_3_SEQUENCE=TCACAGAATCGATTTCGTCCTA
PRIMER_RIGHT_3_SEQUENCE=GAATTTCTCGGAAAGGTGATTG
PRIMER_LEFT_3=141,22
PRIMER_RIGHT_3=327,22
PRIMER_LEFT_3_TM=59.708
PRIMER_RIGHT_3_TM=59.945
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.3688
PRIMER_LEFT_4_PENALTY=0.348961
PRIMER_RIGHT_4_PENALTY=0.019805
PRIMER_LEFT_4_SEQUENCE=AAACCCTCCTTCAGACCTCAAT
PRIMER_RIGHT_4_SEQUENCE=GTTTGAATTTCTCGGAAAGGTG
PRIMER_LEFT_4=200,22
PRIMER_RIGHT_4=331,22
PRIMER_LEFT_4_TM=60.349
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_01410 Ps_contig_mira-and-tgicl-ass_franssen_13658
SEQUENCE=GGTTGCTGCGGTTATATAGAGTTTGTTGTGATTTTGGAGAAGATGGAAGAGAGGTTGAAGATGAATGTGGCAATGAAAGAGAGAGAGCTTCTGAACGGCGTAAGGTTTTGTATCCGCGTTTCGTTCGCGTTTGGGTTGACGCCGCTCATGAACT
TARGET=78,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1406
PRIMER_LEFT_PENALTY=2.045081
PRIMER_RIGHT_PENALTY=0.095566
PRIMER_LEFT_SEQUENCE=GGTTGCTGCGGTTATATAGAGTTT
PRIMER_RIGHT_SEQUENCE=GATACAAAACCTTACGCCGTTC
PRIMER_LEFT=0,24
PRIMER_RIGHT=113,22
PRIMER_LEFT_TM=59.955
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=2.3023
PRIMER_LEFT_1_PENALTY=2.206703
PRIMER_RIGHT_1_PENALTY=0.095566
PRIMER_LEFT_1_SEQUENCE=GGTTGCTGCGGTTATATAGAGTT
PRIMER_RIGHT_1_SEQUENCE=GATACAAAACCTTACGCCGTTC
PRIMER_LEFT_1=0,23
PRIMER_RIGHT_1=113,22
PRIMER_LEFT_1_TM=58.793
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=2.5617
PRIMER_LEFT_2_PENALTY=2.206703
PRIMER_RIGHT_2_PENALTY=0.355025
PRIMER_LEFT_2_SEQUENCE=GGTTGCTGCGGTTATATAGAGTT
PRIMER_RIGHT_2_SEQUENCE=GAAACGCGGATACAAAACCTTA
PRIMER_LEFT_2=0,23
PRIMER_RIGHT_2=121,22
PRIMER_LEFT_2_TM=58.793
PRIMER_RIGHT_2_TM=60.355
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=2.5797
PRIMER_LEFT_3_PENALTY=2.484135
PRIMER_RIGHT_3_PENALTY=0.095566
PRIMER_LEFT_3_SEQUENCE=GGTTGCTGCGGTTATATAGAGT
PRIMER_RIGHT_3_SEQUENCE=GATACAAAACCTTACGCCGTTC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=113,22
PRIMER_LEFT_3_TM=57.516
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=2.5878
PRIMER_LEFT_4_PENALTY=2.045081
PRIMER_RIGHT_4_PENALTY=0.542749
PRIMER_LEFT_4_SEQUENCE=GGTTGCTGCGGTTATATAGAGTTT
PRIMER_RIGHT_4_SEQUENCE=AAACGCGGATACAAAACCTTAC
PRIMER_LEFT_4=0,24
PRIMER_RIGHT_4=120,22
PRIMER_LEFT_4_TM=59.955
PRIMER_RIGHT_4_TM=59.457
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_01411 Ps_contig_mira-and-tgicl-ass_franssen_13667
SEQUENCE=CTACTTTTCTCTTTCTTGTACCTTCTTTTCACCTTATCCAAAACACTTCTACACTGTGAAACGTCCCGTTCGACTTTTAACTCTTCCGAAACTTTCTCAGAAACCTCATGCCATTCCTCCGATCTCAAACTGTTTCTCCCAATTTGAAGAAACTTTTCCCCCCAAATCTCCAACAACACAAAAGTCGCATGTTCTGTCCATTCCTCAGAACCAGAAGAAGAACCCCCACCACCAGAACCACCACCACCGCGATTAACGTATGAGAATTTGCCACGTGGAACCAATTCATAGTTTGAAACAGAAGAACCACCACCACCACCACCACTACCACCGCTTACGTAATTTTCTCTTCTTCCTAATGAACCCTTCGTTTTCCTTCAAAATTAA
TARGET=237,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1015
PRIMER_LEFT_PENALTY=0.081185
PRIMER_RIGHT_PENALTY=0.020269
PRIMER_LEFT_SEQUENCE=ACAACACAAAAGTCGCATGTTC
PRIMER_RIGHT_SEQUENCE=CGTGGCAAATTCTCATACGTTA
PRIMER_LEFT=172,22
PRIMER_RIGHT=274,22
PRIMER_LEFT_TM=60.081
PRIMER_RIGHT_TM=60.020
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=103
PRIMER_PAIR_PENALTY_1=0.1046
PRIMER_LEFT_1_PENALTY=0.081185
PRIMER_RIGHT_1_PENALTY=0.023406
PRIMER_LEFT_1_SEQUENCE=ACAACACAAAAGTCGCATGTTC
PRIMER_RIGHT_1_SEQUENCE=GAGAAAATTACGTAAGCGGTGG
PRIMER_LEFT_1=172,22
PRIMER_RIGHT_1=348,22
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=60.023
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.1335
PRIMER_LEFT_2_PENALTY=0.110131
PRIMER_RIGHT_2_PENALTY=0.023406
PRIMER_LEFT_2_SEQUENCE=GTCCATTCCTCAGAACCAGAAG
PRIMER_RIGHT_2_SEQUENCE=GAGAAAATTACGTAAGCGGTGG
PRIMER_LEFT_2=195,22
PRIMER_RIGHT_2=348,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.023
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=0.1345
PRIMER_LEFT_3_PENALTY=0.111077
PRIMER_RIGHT_3_PENALTY=0.023406
PRIMER_LEFT_3_SEQUENCE=CATGTTCTGTCCATTCCTCAGA
PRIMER_RIGHT_3_SEQUENCE=GAGAAAATTACGTAAGCGGTGG
PRIMER_LEFT_3=187,22
PRIMER_RIGHT_3=348,22
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=60.023
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=0.2312
PRIMER_LEFT_4_PENALTY=0.210973
PRIMER_RIGHT_4_PENALTY=0.020269
PRIMER_LEFT_4_SEQUENCE=CAACACAAAAGTCGCATGTTCT
PRIMER_RIGHT_4_SEQUENCE=CGTGGCAAATTCTCATACGTTA
PRIMER_LEFT_4=173,22
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=60.211
PRIMER_RIGHT_4_TM=60.020
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=102
=
PRIMER_SEQUENCE_ID=SSR_01412 Ps_contig_mira-and-tgicl-ass_franssen_13667
SEQUENCE=CTACTTTTCTCTTTCTTGTACCTTCTTTTCACCTTATCCAAAACACTTCTACACTGTGAAACGTCCCGTTCGACTTTTAACTCTTCCGAAACTTTCTCAGAAACCTCATGCCATTCCTCCGATCTCAAACTGTTTCTCCCAATTTGAAGAAACTTTTCCCCCCAAATCTCCAACAACACAAAAGTCGCATGTTCTGTCCATTCCTCAGAACCAGAAGAAGAACCCCCACCACCAGAACCACCACCACCGCGATTAACGTATGAGAATTTGCCACGTGGAACCAATTCATAGTTTGAAACAGAAGAACCACCACCACCACCACCACTACCACCGCTTACGTAATTTTCTCTTCTTCCTAATGAACCCTTCGTTTTCCTTCAAAATTAA
TARGET=306,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1046
PRIMER_LEFT_PENALTY=0.081185
PRIMER_RIGHT_PENALTY=0.023406
PRIMER_LEFT_SEQUENCE=ACAACACAAAAGTCGCATGTTC
PRIMER_RIGHT_SEQUENCE=GAGAAAATTACGTAAGCGGTGG
PRIMER_LEFT=172,22
PRIMER_RIGHT=348,22
PRIMER_LEFT_TM=60.081
PRIMER_RIGHT_TM=60.023
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.1335
PRIMER_LEFT_1_PENALTY=0.110131
PRIMER_RIGHT_1_PENALTY=0.023406
PRIMER_LEFT_1_SEQUENCE=GTCCATTCCTCAGAACCAGAAG
PRIMER_RIGHT_1_SEQUENCE=GAGAAAATTACGTAAGCGGTGG
PRIMER_LEFT_1=195,22
PRIMER_RIGHT_1=348,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.023
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=154
PRIMER_PAIR_PENALTY_2=0.1345
PRIMER_LEFT_2_PENALTY=0.111077
PRIMER_RIGHT_2_PENALTY=0.023406
PRIMER_LEFT_2_SEQUENCE=CATGTTCTGTCCATTCCTCAGA
PRIMER_RIGHT_2_SEQUENCE=GAGAAAATTACGTAAGCGGTGG
PRIMER_LEFT_2=187,22
PRIMER_RIGHT_2=348,22
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=60.023
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=0.2344
PRIMER_LEFT_3_PENALTY=0.210973
PRIMER_RIGHT_3_PENALTY=0.023406
PRIMER_LEFT_3_SEQUENCE=CAACACAAAAGTCGCATGTTCT
PRIMER_RIGHT_3_SEQUENCE=GAGAAAATTACGTAAGCGGTGG
PRIMER_LEFT_3=173,22
PRIMER_RIGHT_3=348,22
PRIMER_LEFT_3_TM=60.211
PRIMER_RIGHT_3_TM=60.023
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=0.3046
PRIMER_LEFT_4_PENALTY=0.281144
PRIMER_RIGHT_4_PENALTY=0.023406
PRIMER_LEFT_4_SEQUENCE=CGTTCGACTTTTAACTCTTCCG
PRIMER_RIGHT_4_SEQUENCE=GAGAAAATTACGTAAGCGGTGG
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=348,22
PRIMER_LEFT_4_TM=60.281
PRIMER_RIGHT_4_TM=60.023
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_01413 Ps_contig_mira-and-tgicl-ass_franssen_13692
SEQUENCE=ACAAACAAAGAATGTGATTCCCGCAAAGAGCTAAAACTGGAAGAACATTCCAAATTGGAAGTGGAACAATCACGAGACGATCCAAGGAATGAAAATTTAATCAGACGCATCRGTAGAGAAAGAAGCGACCGCAACAGCAGCAGCAGCCACAAGGGGTCTCTGAATTGGTCCAGAAAAATAACTCATTACAGAAACCTTTAACCCTTAATAATATCACAAATTGAATGAAATTGGCAAGACCCAGAATGAAATCAAGTGGAAAAACG
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01414 Ps_contig_mira-and-tgicl-ass_franssen_13709
SEQUENCE=ATTGCCATTGGAACATTGAGACGAAGCATCTCTGCAATATTCAGGATTTCAGGATCATCCGGCTAAATTCTTTCTTTCTAATGCAAATGGAGACGGAGAGTTCCAACTCCTTCGGCTTACTCTTCGGCGTCCGGCTTCTGACTTTCCGATACTCCCTCWGTCCGCCGGCGTTTGCACGGACTCCCCGAAACGGTTGCGACTGCTGCTTCTTGACGCTGTTATCGAAAAGGAGGAAAGAGACACGTCGAGGAGGGTTACATGAAGGGAATGCATTA
TARGET=68,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01415 Ps_contig_mira-and-tgicl-ass_franssen_13722
SEQUENCE=TTGCATATAGTGAAGGGATTCGTTTTGATAAGATCAACAGTGAATCAGAGTAATCATCTAGTTGGAATGTTAGAGTTCCCAGAGTTGTATTATTTCCTTACTCCGTGTCCGATTTTTACAGATACAGCAGTTATTTTGCTTATGGACAATCGAAACTAGCAAACATTTTGCATGCCAATGAGCTTGCAAGGCGCTTGGAAGGAAGGAACGGGTGACAAATAACAGTTAACTCTCTTCATCCGTGGTACAATTATTACAAATATTCTGCGGTACCATGGTTATTTTAATGCCGTTGCTAATA
TARGET=197,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7207
PRIMER_LEFT_PENALTY=0.355872
PRIMER_RIGHT_PENALTY=0.364827
PRIMER_LEFT_SEQUENCE=TTATTTCCTTACTCCGTGTCCG
PRIMER_RIGHT_SEQUENCE=AATAACCATGGTACCGCAGAAT
PRIMER_LEFT=89,22
PRIMER_RIGHT=282,22
PRIMER_LEFT_TM=60.356
PRIMER_RIGHT_TM=59.635
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=0.7694
PRIMER_LEFT_1_PENALTY=0.404566
PRIMER_RIGHT_1_PENALTY=0.364827
PRIMER_LEFT_1_SEQUENCE=ACTCCGTGTCCGATTTTTACAG
PRIMER_RIGHT_1_SEQUENCE=AATAACCATGGTACCGCAGAAT
PRIMER_LEFT_1=99,22
PRIMER_RIGHT_1=282,22
PRIMER_LEFT_1_TM=60.405
PRIMER_RIGHT_1_TM=59.635
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.8511
PRIMER_LEFT_2_PENALTY=0.486257
PRIMER_RIGHT_2_PENALTY=0.364827
PRIMER_LEFT_2_SEQUENCE=TACTCCGTGTCCGATTTTTACA
PRIMER_RIGHT_2_SEQUENCE=AATAACCATGGTACCGCAGAAT
PRIMER_LEFT_2=98,22
PRIMER_RIGHT_2=282,22
PRIMER_LEFT_2_TM=59.514
PRIMER_RIGHT_2_TM=59.635
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.9514
PRIMER_LEFT_3_PENALTY=0.355872
PRIMER_RIGHT_3_PENALTY=0.595491
PRIMER_LEFT_3_SEQUENCE=TTATTTCCTTACTCCGTGTCCG
PRIMER_RIGHT_3_SEQUENCE=AAAATAACCATGGTACCGCAGA
PRIMER_LEFT_3=89,22
PRIMER_RIGHT_3=284,22
PRIMER_LEFT_3_TM=60.356
PRIMER_RIGHT_3_TM=60.595
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=0.9514
PRIMER_LEFT_4_PENALTY=0.355872
PRIMER_RIGHT_4_PENALTY=0.595491
PRIMER_LEFT_4_SEQUENCE=TTATTTCCTTACTCCGTGTCCG
PRIMER_RIGHT_4_SEQUENCE=AAATAACCATGGTACCGCAGAA
PRIMER_LEFT_4=89,22
PRIMER_RIGHT_4=283,22
PRIMER_LEFT_4_TM=60.356
PRIMER_RIGHT_4_TM=60.595
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_01416 Ps_contig_mira-and-tgicl-ass_franssen_13739
SEQUENCE=TCAACGCAGAGTATATGTTATTATAGTAGTTTTGTTWATTCAGTTGTGACCTGGATTTGAGTTAAAATGTGTTATGCAGTTAGATGGTTTTGAATAATGTTAGTAGAAAATCAATGAAGTTAGTTGTTGAAGTTAGTGTAAGTGAATTAGAACTATCAGTTTGGATTTTGTTAGTTAGTTAATTAGTTTAATTAAAATTATTTTAATTTGGAAGGTGTTGTGAACAATGTCATTATGTAATGGATAATACTTCTAACCAGATACATTCTSCAATGACTCTTTCTCTACCTNTATAAAAGGAGCTGGAGTTTNAAGAGG
TARGET=170,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01417 Ps_contig_mira-and-tgicl-ass_franssen_13743
SEQUENCE=CACTGTTGGCTTTGCAACACACCAAATAGCATAGTGTGGTGGTGAGCTTTTATKGAGGTGGTGGTGGTGGATAAGCCATAGGTGGGCTTATAACAACTTTAGGTGGGCTTGGAACATGTTTTGGCGGGCTTGGAGGTGGTGGTGTTGGTGGTGGTGAAATTGTAACAATGGTTTTTGGACTTGGTGGTGGGTTTGGAAGAGAGGAATAAGGTTGTGGTGCAAGTGAATTGAAAGGTGGAAATGGTTGAGAAGAAGGAGAACCATATGAGTCAATGTCTAAGTATTCAAATGATGAAAATTTGTTTATACTGTTTCGAGATTCTAGTTTCTTCCATGATTTGATGGAGGGGCTTCTACTAATTATACCTCCTCCATGTTTTATTGCACCAGACAGAGGAAAAAGAGTGGAAACTAGGATCAAGAGGCAAAGATGTGCAATGCTGACTCTCCCG
TARGET=57,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01418 Ps_contig_mira-and-tgicl-ass_franssen_13746
SEQUENCE=CTTATTCTCTTCTCATCACAACAACACTTTTAACTACTACCTCATCATCTCATCACCCTTCTTGCATCAATTCCCTTCCTCACATCAACAAAATTCAGAAAATCCATTTCCTCTCCCTCACTACAATGTAACAATTTACACTCAAACAACAACCCCTTAAAACCTCCTTTCTTTACCGGCGACAGAAGCTTCACCACTAGACCACCAACTTCGAACTACCGCAAAAAACCATTCTTCTTCTTCCTCTTCCAATGGAACCGCCACCCATACATAAAAAATCGGTACCACGAAACAGCCTCACCACCTTAAGAAATCAATTTCACTCTTAAACCCTCCCTCTAAATTCAATGATCCAAACATAAACCGTCATGACGTTCAAATTGTTTCCTCATTAACCGATCCGCTCGGCTACAACTCCCACATCTGTC
TARGET=232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3584
PRIMER_LEFT_PENALTY=0.174518
PRIMER_RIGHT_PENALTY=0.183834
PRIMER_LEFT_SEQUENCE=GAAGCTTCACCACTAGACCACC
PRIMER_RIGHT_SEQUENCE=GGATCGGTTAATGAGGAAACAA
PRIMER_LEFT=184,22
PRIMER_RIGHT=401,22
PRIMER_LEFT_TM=60.175
PRIMER_RIGHT_TM=60.184
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=0.3691
PRIMER_LEFT_1_PENALTY=0.204533
PRIMER_RIGHT_1_PENALTY=0.164560
PRIMER_LEFT_1_SEQUENCE=CAACCCCTTAAAACCTCCTTTC
PRIMER_RIGHT_1_SEQUENCE=TTTCTTAAGGTGGTGAGGCTGT
PRIMER_LEFT_1=149,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=60.205
PRIMER_RIGHT_1_TM=60.165
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.3691
PRIMER_LEFT_2_PENALTY=0.204533
PRIMER_RIGHT_2_PENALTY=0.164560
PRIMER_LEFT_2_SEQUENCE=CAACCCCTTAAAACCTCCTTTC
PRIMER_RIGHT_2_SEQUENCE=TTCTTAAGGTGGTGAGGCTGTT
PRIMER_LEFT_2=149,22
PRIMER_RIGHT_2=311,22
PRIMER_LEFT_2_TM=60.205
PRIMER_RIGHT_2_TM=60.165
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=0.3691
PRIMER_LEFT_3_PENALTY=0.204533
PRIMER_RIGHT_3_PENALTY=0.164560
PRIMER_LEFT_3_SEQUENCE=CAACCCCTTAAAACCTCCTTTC
PRIMER_RIGHT_3_SEQUENCE=CTTAAGGTGGTGAGGCTGTTTC
PRIMER_LEFT_3=149,22
PRIMER_RIGHT_3=309,22
PRIMER_LEFT_3_TM=60.205
PRIMER_RIGHT_3_TM=60.165
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=0.3856
PRIMER_LEFT_4_PENALTY=0.220992
PRIMER_RIGHT_4_PENALTY=0.164560
PRIMER_LEFT_4_SEQUENCE=CCCTTCCTCACATCAACAAAAT
PRIMER_RIGHT_4_SEQUENCE=CTTAAGGTGGTGAGGCTGTTTC
PRIMER_LEFT_4=72,22
PRIMER_RIGHT_4=309,22
PRIMER_LEFT_4_TM=60.221
PRIMER_RIGHT_4_TM=60.165
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_01419 Ps_contig_mira-and-tgicl-ass_franssen_13786
SEQUENCE=CGGGGGGAACTACGTCGCCCTGATCCTCATACCATATGAGGTACGTAGGCAGGAGATTGAGCTGATCTCTCCGGGCACTTTCTTTTCTTTTTGTGTGTGTTTGTTATCCTTTTCTGGTTGGAGTCTGATGTAAGTCCAGCGATTGGCAGTCAGTTTCCTGTGTGTTTGTGGTGTGTTGGAGTCTGACGTAAGTCCAGCGATTGGCAGTCGGTTTCCTGTGTCTTGTTTGTTTGTGCGGAGTCTGACATAAGTCCAGCGGTTGGCAGTCGGTTTCCTGTTTGCGTTTTGTTTGGCGTGCGTTAGCCGAGCTACGAGTGCTCTGATTCTTTCTCTGGTTAGAGAAGATACGTATGCATAGGATGCGACATCCTAGCGAGCACGTTTCCCCTGTCCTCGAACTACGTCGACTCTGATGTTTGTGTCTGACAAACTACGTAGTCCCAGGATGCGATATCCTGCCGAGTCCGTTTCTTCCGTCCTTTCTGTGTTTGTTTCCAGTTTTGTGCAGTTTTGAGAAGTATCTTTAGCAACCTTTCTTCTATTCTTTCTGAGCGTGGATCCCGTCGAGTACGACGGATGCGTAGGGGTGCTAATACCTTCCCTTCGCATAACCGACTCCCGATCCCATCAATCTCTGGTCGCGAGACCATTTCCTTTCCAGGTTTACTTCGAGCGTTTCCTTTCCCGCCTTTGGGATAAATAACGCACGGTGGCGGCTCTGTGTTTCTTTTCCCGCCGGTTTTTCGCGTAATGCGACACACACAATGGGAGAAAAGGAAAGAAGATAATCAGAAGTGTTGGAGAAGAAAAACAGAGGCTTTCTTGTGTATTCCATTTGCTGTCAAACATGGTGATGAGGAGCTAAACCCCACTTTGTCAAGATTGGAGGTAGTAGTTATCCCTTATTGATTATGTATTCCTATTGACTCTTGTATGTGACAAAAGATTATTGATGTAATGATTGATATTTTCACCCTTATTTTGAATTATTTCAGATTTGAARAGTT
TARGET=223,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01420 Ps_contig_mira-and-tgicl-ass_franssen_13802
SEQUENCE=CGTAATWATGAATAAGTGAATGATTTAATAAAAAATGCGACTCATATTCAATGTAATGTGGTTTACACTTTACACCAGAAAATTCCCAGAACTCTCCAACAAAAGAGATGAAGATTCTCAAAACATAAAACACTAAGCCAATTAACGAACCAAAATTGCTGATAATAATAATACGAGGAACAAAAACAAACTGCACTAGGAAACCTTGTGATGCTAAATATAAAATACATATTCTACCTCAAAGACCGAAATCGTGCCTGACAAGAATAACTTGACTTATTTCTTATTTTCATCAACCAGCTCTTTTACACAAAATTGATGAAGGTTTTCATACATTTTCTTCCATTGATTAACCATCTGCACAGACTTTTGGTAATCGCTTTTTAGTTTCTCTATCTCCATTTCAGAAGCGGCAGCTGAACCTTGATTCTGAAGTTCTTTACTGTAACTAGAAATCACATGAGAACTAAGGTATGAAGCGTCCATGCATCTATCACTATCCCCTAGACTGACGATAGTCTTTGTATCTGAATGCCCGTCTATCGAATTTGCATATTTTTCCAAAGGAGGAAATTGAGCTCCCTTCTTTTTTGAGGCTGCTTGTAAATTAGATGACATATGATTACTTGAATCATTTTCTGGTCTAACAACAATAATATTGGTTATTGGTCCTTTAGCATGTTTAAAGACACGGATGATGTTATGAGTACTTGCGTTCCAAACTCGGACTATTCCATCCTCAGATCCCGATATTAAAAAGTTATCACTGGCGCTATATGCCAAGCAAGTAACTGCCTTGCTGTGGTTAGAAAATGAATCAACGATATAAGAAACGTGATTATTGGTAGCGATTTTTGTGGTGTTAAGTGCAACGATAAATATTTTTCCATCTTCACTGCCAGTATAAAAGACATGTTCAGCAGGATCCAAAGCAATAGCTTTAATTTTTGAAGGGAATACTATATTTCTTTGTAGCATTCCATTAGATAAGCTCCATACCTTACAAGTCCGATCATCTGATGCTGAAACTATAATTGCATTGCACCCTCCAGAACCAATCACAACATCATTTACACACAAAGTGTGATCTGAAAAACTATACTCGTAAATCTGGCTTGCTTCCCGTCTTCTTACATCATCAAATATCATTAAGAGAGACCAAACTCGTACGCTTCCATTTTTTAAAACCAGATATTAGAAGTGAATCGTCTTCAGAAAATACAAGGCAAGTAACAGCCGTATTATGACCATGCCACTTCTTAAGCAATTTGCCGGTTTCAACCTCCCACAAGTATATTTCACCGGACAAACCTCCACCAACAAGATAGGTGCCACTGTGATTAGCAGCGAGAGGCGTAATAGGTTCGGCGGGAAAGCTCTTTTACCTTC
TARGET=162,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01421 Ps_contig_mira-and-tgicl-ass_franssen_13816
SEQUENCE=GAAATCCAACTCACTCAAATATCTCTCTCTCTCTCTCTAGTATTCTAGTTAAGGAAAAWGACAAGAACGTAACGTTTAAAGGTCGTAACCCTTAAACCTTACCTTTTCGTTTCCGTTTCCCTCTACTAAGTTGGT
TARGET=22,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01422 Ps_contig_mira-and-tgicl-ass_franssen_13831
SEQUENCE=GTTTTCCTACTTACTAAACGACCTCCGCCACGTGGAAGGTACCTCCGTCCTTCCTAAAAGAATAATCAACCGCCAGTGCATCCAAAGACATTGTATCTTTTTCCTACAGTTGGTACATATATATTCTTGAACAGTGTTTTTGTTCTCCAATTCATCTTTCAGCTTATGCTTCATGCGATGCAGTCTGTACCTGACCACATCATATATCTGTGCATAATCTAGACAACAGTAAGAGTGTGTGTGTAGCTTGTACCTTTTCTTCTCCCTCCTTTCCTGTTGCAGCATCAGCTGTAGCACGCTACNCGCCCGCTACTTATTATTTA
TARGET=235,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1125
PRIMER_LEFT_PENALTY=0.034674
PRIMER_RIGHT_PENALTY=0.077784
PRIMER_LEFT_SEQUENCE=CCTCCGTCCTTCCTAAAAGAAT
PRIMER_RIGHT_SEQUENCE=TAAATAATAAGTAGCGGGCGNG
PRIMER_LEFT=41,22
PRIMER_RIGHT=322,22
PRIMER_LEFT_TM=59.965
PRIMER_RIGHT_TM=59.922
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=11.5250
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.4257
PRIMER_LEFT_1_PENALTY=0.347892
PRIMER_RIGHT_1_PENALTY=0.077784
PRIMER_LEFT_1_SEQUENCE=GCAGTCTGTACCTGACCACATC
PRIMER_RIGHT_1_SEQUENCE=TAAATAATAAGTAGCGGGCGNG
PRIMER_LEFT_1=179,22
PRIMER_RIGHT_1=322,22
PRIMER_LEFT_1_TM=59.652
PRIMER_RIGHT_1_TM=59.922
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=11.5250
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=0.4257
PRIMER_LEFT_2_PENALTY=0.347892
PRIMER_RIGHT_2_PENALTY=0.077784
PRIMER_LEFT_2_SEQUENCE=GATGCAGTCTGTACCTGACCAC
PRIMER_RIGHT_2_SEQUENCE=TAAATAATAAGTAGCGGGCGNG
PRIMER_LEFT_2=176,22
PRIMER_RIGHT_2=322,22
PRIMER_LEFT_2_TM=59.652
PRIMER_RIGHT_2_TM=59.922
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=11.5250
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=0.4303
PRIMER_LEFT_3_PENALTY=0.352523
PRIMER_RIGHT_3_PENALTY=0.077784
PRIMER_LEFT_3_SEQUENCE=AGGTACCTCCGTCCTTCCTAAA
PRIMER_RIGHT_3_SEQUENCE=TAAATAATAAGTAGCGGGCGNG
PRIMER_LEFT_3=36,22
PRIMER_RIGHT_3=322,22
PRIMER_LEFT_3_TM=60.353
PRIMER_RIGHT_3_TM=59.922
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=11.5250
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=0.4303
PRIMER_LEFT_4_PENALTY=0.352523
PRIMER_RIGHT_4_PENALTY=0.077784
PRIMER_LEFT_4_SEQUENCE=AAGGTACCTCCGTCCTTCCTAA
PRIMER_RIGHT_4_SEQUENCE=TAAATAATAAGTAGCGGGCGNG
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=322,22
PRIMER_LEFT_4_TM=60.353
PRIMER_RIGHT_4_TM=59.922
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=11.5250
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_01423 Ps_contig_mira-and-tgicl-ass_franssen_13835
SEQUENCE=GACAAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAGACTTGCTCTTCACAAACAATCATCGACAAAGAACACCAGCACAGTGCCCACAATTACCATCCACTTCCCATTGTTTTTGCT
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6320
PRIMER_LEFT_PENALTY=1.577079
PRIMER_RIGHT_PENALTY=0.054900
PRIMER_LEFT_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_SEQUENCE=AATGGGAAGTGGATGGTAATTG
PRIMER_LEFT=7,21
PRIMER_RIGHT=143,22
PRIMER_LEFT_TM=60.577
PRIMER_RIGHT_TM=59.945
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=1.6320
PRIMER_LEFT_1_PENALTY=1.577079
PRIMER_RIGHT_1_PENALTY=0.054900
PRIMER_LEFT_1_SEQUENCE=AAAAACACACCAAGCCAGTGA
PRIMER_RIGHT_1_SEQUENCE=AATGGGAAGTGGATGGTAATTG
PRIMER_LEFT_1=6,21
PRIMER_RIGHT_1=143,22
PRIMER_LEFT_1_TM=60.577
PRIMER_RIGHT_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=1.8769
PRIMER_LEFT_2_PENALTY=1.821959
PRIMER_RIGHT_2_PENALTY=0.054900
PRIMER_LEFT_2_SEQUENCE=AAAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_2_SEQUENCE=AATGGGAAGTGGATGGTAATTG
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=143,22
PRIMER_LEFT_2_TM=61.822
PRIMER_RIGHT_2_TM=59.945
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=138
PRIMER_PAIR_PENALTY_3=2.0400
PRIMER_LEFT_3_PENALTY=1.577079
PRIMER_RIGHT_3_PENALTY=0.462913
PRIMER_LEFT_3_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_3_SEQUENCE=AACAATGGGAAGTGGATGGTAA
PRIMER_LEFT_3=7,21
PRIMER_RIGHT_3=146,22
PRIMER_LEFT_3_TM=60.577
PRIMER_RIGHT_3_TM=60.463
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=140
PRIMER_PAIR_PENALTY_4=2.0400
PRIMER_LEFT_4_PENALTY=1.577079
PRIMER_RIGHT_4_PENALTY=0.462913
PRIMER_LEFT_4_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_4_SEQUENCE=AAACAATGGGAAGTGGATGGTA
PRIMER_LEFT_4=7,21
PRIMER_RIGHT_4=147,22
PRIMER_LEFT_4_TM=60.577
PRIMER_RIGHT_4_TM=60.463
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_01424 Ps_contig_mira-and-tgicl-ass_franssen_13835
SEQUENCE=GACAAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAGACTTGCTCTTCACAAACAATCATCGACAAAGAACACCAGCACAGTGCCCACAATTACCATCCACTTCCCATTGTTTTTGCT
TARGET=57,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2552
PRIMER_LEFT_PENALTY=1.200250
PRIMER_RIGHT_PENALTY=0.054900
PRIMER_LEFT_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_SEQUENCE=AATGGGAAGTGGATGGTAATTG
PRIMER_LEFT=8,21
PRIMER_RIGHT=143,22
PRIMER_LEFT_TM=60.200
PRIMER_RIGHT_TM=59.945
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=1.5286
PRIMER_LEFT_1_PENALTY=1.473671
PRIMER_RIGHT_1_PENALTY=0.054900
PRIMER_LEFT_1_SEQUENCE=AAAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_1_SEQUENCE=AATGGGAAGTGGATGGTAATTG
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=143,22
PRIMER_LEFT_1_TM=61.474
PRIMER_RIGHT_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=1.6320
PRIMER_LEFT_2_PENALTY=1.577079
PRIMER_RIGHT_2_PENALTY=0.054900
PRIMER_LEFT_2_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_2_SEQUENCE=AATGGGAAGTGGATGGTAATTG
PRIMER_LEFT_2=7,21
PRIMER_RIGHT_2=143,22
PRIMER_LEFT_2_TM=60.577
PRIMER_RIGHT_2_TM=59.945
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=1.6320
PRIMER_LEFT_3_PENALTY=1.577079
PRIMER_RIGHT_3_PENALTY=0.054900
PRIMER_LEFT_3_SEQUENCE=AAAAACACACCAAGCCAGTGA
PRIMER_RIGHT_3_SEQUENCE=AATGGGAAGTGGATGGTAATTG
PRIMER_LEFT_3=6,21
PRIMER_RIGHT_3=143,22
PRIMER_LEFT_3_TM=60.577
PRIMER_RIGHT_3_TM=59.945
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=1.6632
PRIMER_LEFT_4_PENALTY=1.200250
PRIMER_RIGHT_4_PENALTY=0.462913
PRIMER_LEFT_4_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_4_SEQUENCE=AACAATGGGAAGTGGATGGTAA
PRIMER_LEFT_4=8,21
PRIMER_RIGHT_4=146,22
PRIMER_LEFT_4_TM=60.200
PRIMER_RIGHT_4_TM=60.463
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_01425 Ps_contig_mira-and-tgicl-ass_franssen_13869
SEQUENCE=CGAGAGCATCAAGCTTCTGAGATACACCTGTTCAGAAAATAGTGATTATTAAGCATAATCAGTTAATTCAGTATAGTAAAAGCGATTTTTGGATTAACATATGAATGCATTAGGAAAGGGATTGTAAGAGCGTGGGTTTGGGAAGAAGAAGAAGAAAAATGGAATTATAGAGGATGAAAATGAAGAGAGGAATCGAAGAGAACCTTGAAGAGAAAAGAGTATTGAAATTAGGGTGTGGAAAATGGGAATGATGGTCCCCGCGTAGAGTTTCGCCC
TARGET=142,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3652
PRIMER_LEFT_PENALTY=0.191886
PRIMER_RIGHT_PENALTY=0.173354
PRIMER_LEFT_SEQUENCE=GAATGCATTAGGAAAGGGATTG
PRIMER_RIGHT_SEQUENCE=TCAAGGTTCTCTTCGATTCCTC
PRIMER_LEFT=102,22
PRIMER_RIGHT=207,22
PRIMER_LEFT_TM=59.808
PRIMER_RIGHT_TM=59.827
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=0.6039
PRIMER_LEFT_1_PENALTY=0.191886
PRIMER_RIGHT_1_PENALTY=0.412035
PRIMER_LEFT_1_SEQUENCE=GAATGCATTAGGAAAGGGATTG
PRIMER_RIGHT_1_SEQUENCE=CCATTTTCCACACCCTAATTTC
PRIMER_LEFT_1=102,22
PRIMER_RIGHT_1=244,22
PRIMER_LEFT_1_TM=59.808
PRIMER_RIGHT_1_TM=59.588
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.6598
PRIMER_LEFT_2_PENALTY=0.247794
PRIMER_RIGHT_2_PENALTY=0.412035
PRIMER_LEFT_2_SEQUENCE=TAGGAAAGGGATTGTAAGAGCG
PRIMER_RIGHT_2_SEQUENCE=CCATTTTCCACACCCTAATTTC
PRIMER_LEFT_2=110,22
PRIMER_RIGHT_2=244,22
PRIMER_LEFT_2_TM=59.752
PRIMER_RIGHT_2_TM=59.588
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=135
PRIMER_PAIR_PENALTY_3=0.8436
PRIMER_LEFT_3_PENALTY=0.191886
PRIMER_RIGHT_3_PENALTY=0.651681
PRIMER_LEFT_3_SEQUENCE=GAATGCATTAGGAAAGGGATTG
PRIMER_RIGHT_3_SEQUENCE=CTTCAAGGTTCTCTTCGATTCC
PRIMER_LEFT_3=102,22
PRIMER_RIGHT_3=209,22
PRIMER_LEFT_3_TM=59.808
PRIMER_RIGHT_3_TM=59.348
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=108
PRIMER_PAIR_PENALTY_4=0.8964
PRIMER_LEFT_4_PENALTY=0.484334
PRIMER_RIGHT_4_PENALTY=0.412035
PRIMER_LEFT_4_SEQUENCE=AGGAAAGGGATTGTAAGAGCGT
PRIMER_RIGHT_4_SEQUENCE=CCATTTTCCACACCCTAATTTC
PRIMER_LEFT_4=111,22
PRIMER_RIGHT_4=244,22
PRIMER_LEFT_4_TM=60.484
PRIMER_RIGHT_4_TM=59.588
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_01426 Ps_contig_mira-and-tgicl-ass_franssen_13871
SEQUENCE=GTAATCCTTATCCATAACCTTARTGCTTTCTGAGTTTTCGCGACAAAGAGGTGTCTCTTGAAGCTCTAGTTCAACACCTTCAGATTTGTCATTTGGTAGTGTAATTTCACAATGGAAGCATTTCAATGCAGATGCTTTTATAGTTTGAAACATGTTCATGAAAGGCTTCTTATCTTGAAGACCATCATCATCATCCTGTTTTTATATGAATATATCGAACTTCCGNTAGAGAAAATCAAGATAAGAAAGAAGCTAA
TARGET=183,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01427 Ps_contig_mira-and-tgicl-ass_franssen_13877
SEQUENCE=GTCGGGGAATCTCAAACACTATAACAAGCTATATTGTGAGTGTGAAAAATCCACTATCACACCATAGAGAATAACAACAACAACAAAAATAGTGTGTGAAACCACAAAAAAAACTAAGAGAGTGGAAGTAATATAGACATTTTGAAGAGAATGGAGATGGCTTCAGATAAAGTTGTGGAAACAGTAATAGTTGGAAACTATGAAGAAATGGAAAATGAAGGAAAGCCACAAGACATAAAATCCAAGTTATCAAACTTCTTATGGCATGGTGGTTCTGCTTATGACGCTTGGTTTAGTTGTGCTTCCAACCAGGTGGCTCAAGTTCTGCTTACGTTGCCATACTCATTTTCTCAACTGGGAATGCTTTCTGGCATTCTTTTTCAACTCTTCTATGGCTTACTTGGTAGCTGGACTGCTTACCTCATTAGCATTCTCTATGTTGAATACAAGAACAAGAAAAGAGAGAAGAAAAAAGTTAATTT
TARGET=73,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6518
PRIMER_LEFT_PENALTY=0.647458
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=GTCGGGGAATCTCAAACACTAT
PRIMER_RIGHT_SEQUENCE=ATGTCTTGTGGCTTTCCTTCAT
PRIMER_LEFT=0,22
PRIMER_RIGHT=235,22
PRIMER_LEFT_TM=59.353
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.3000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.7368
PRIMER_LEFT_1_PENALTY=0.647458
PRIMER_RIGHT_1_PENALTY=0.089381
PRIMER_LEFT_1_SEQUENCE=GTCGGGGAATCTCAAACACTAT
PRIMER_RIGHT_1_SEQUENCE=TAAACCAAGCGTCATAAGCAGA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=294,22
PRIMER_LEFT_1_TM=59.353
PRIMER_RIGHT_1_TM=59.911
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=0.7564
PRIMER_LEFT_2_PENALTY=0.647458
PRIMER_RIGHT_2_PENALTY=0.108929
PRIMER_LEFT_2_SEQUENCE=GTCGGGGAATCTCAAACACTAT
PRIMER_RIGHT_2_SEQUENCE=CTTGTGGCTTTCCTTCATTTTC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=231,22
PRIMER_LEFT_2_TM=59.353
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.7990
PRIMER_LEFT_3_PENALTY=0.647458
PRIMER_RIGHT_3_PENALTY=0.151533
PRIMER_LEFT_3_SEQUENCE=GTCGGGGAATCTCAAACACTAT
PRIMER_RIGHT_3_SEQUENCE=CTGAAGCCATCTCCATTCTCTT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=165,22
PRIMER_LEFT_3_TM=59.353
PRIMER_RIGHT_3_TM=59.848
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.8552
PRIMER_LEFT_4_PENALTY=0.647458
PRIMER_RIGHT_4_PENALTY=0.207756
PRIMER_LEFT_4_SEQUENCE=GTCGGGGAATCTCAAACACTAT
PRIMER_RIGHT_4_SEQUENCE=AGCCATCTCCATTCTCTTCAAA
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=161,22
PRIMER_LEFT_4_TM=59.353
PRIMER_RIGHT_4_TM=60.208
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_01428 Ps_contig_mira-and-tgicl-ass_franssen_13923
SEQUENCE=AACTCCATTTTCCTTTTCCTCGGGCCACTTTGATGTATTGCGACAATGTTAGTGTCATCTATCTATCTGGTAATCTTGTGCAGCATCAACGCACTAAACATATTGAGATGGACATTCATTTTGGTTACGGGAAAAAGATAGCTCGTGGTCAGGCTCGCGTTCCTTCATTCGTTCCCTACAA
TARGET=57,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0054
PRIMER_LEFT_PENALTY=0.788802
PRIMER_RIGHT_PENALTY=0.216588
PRIMER_LEFT_SEQUENCE=AACTCCATTTTCCTTTTCCTCG
PRIMER_RIGHT_SEQUENCE=CCTGACCACGAGCTATCTTTTT
PRIMER_LEFT=0,22
PRIMER_RIGHT=152,22
PRIMER_LEFT_TM=60.789
PRIMER_RIGHT_TM=59.783
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=1.1412
PRIMER_LEFT_1_PENALTY=0.788802
PRIMER_RIGHT_1_PENALTY=0.352395
PRIMER_LEFT_1_SEQUENCE=AACTCCATTTTCCTTTTCCTCG
PRIMER_RIGHT_1_SEQUENCE=TGTAGGGAACGAATGAAGGAAC
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=179,22
PRIMER_LEFT_1_TM=60.789
PRIMER_RIGHT_1_TM=60.352
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=1.2688
PRIMER_LEFT_2_PENALTY=0.788802
PRIMER_RIGHT_2_PENALTY=0.480018
PRIMER_LEFT_2_SEQUENCE=AACTCCATTTTCCTTTTCCTCG
PRIMER_RIGHT_2_SEQUENCE=GCTATCTTTTTCCCGTAACCAA
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=141,22
PRIMER_LEFT_2_TM=60.789
PRIMER_RIGHT_2_TM=59.520
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=1.3947
PRIMER_LEFT_3_PENALTY=0.788802
PRIMER_RIGHT_3_PENALTY=0.605851
PRIMER_LEFT_3_SEQUENCE=AACTCCATTTTCCTTTTCCTCG
PRIMER_RIGHT_3_SEQUENCE=CACGAGCTATCTTTTTCCCGTA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=146,22
PRIMER_LEFT_3_TM=60.789
PRIMER_RIGHT_3_TM=60.606
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=1.4010
PRIMER_LEFT_4_PENALTY=0.788802
PRIMER_RIGHT_4_PENALTY=0.612170
PRIMER_LEFT_4_SEQUENCE=AACTCCATTTTCCTTTTCCTCG
PRIMER_RIGHT_4_SEQUENCE=TGACCACGAGCTATCTTTTTCC
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=150,22
PRIMER_LEFT_4_TM=60.789
PRIMER_RIGHT_4_TM=60.612
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_01429 Ps_contig_mira-and-tgicl-ass_franssen_13933
SEQUENCE=CCAAAAAATTCTCTTCCGGAAGAATCTCGCCGCTGAAGCTACTACTTGAACCTCCATTACCACCTACGGAGCCCCCAAACTCCTCTCCACCGGAATCACTGCTGCAATATTCCAAACTTCCTTCAAAGCCCTCGCTTTCAAGGTCGCAGCTCCACGCAAAGCTGTTGCAGCAGCAGCAGTTAAAGTCATGATATCACCGGCTGATCTAACATTCACGGCGGAGCCAACCACGGCAGCAAGATGTTCCCTGTCTGCCCCCAGAGACTCAGCTGCCTCAACGCATTGAGCCGCAACCAACGTCGCTGCAGAGGCCACAGCCGAGTCCGTCTTCGCAATCTGCTCGTCATCACCAGACCCGGAAGAAGCAGCGGTG
TARGET=167,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6501
PRIMER_LEFT_PENALTY=0.364827
PRIMER_RIGHT_PENALTY=1.285229
PRIMER_LEFT_SEQUENCE=GCTGCAATATTCCAAACTTCCT
PRIMER_RIGHT_SEQUENCE=GTCTGGTGATGACGAGCAGAT
PRIMER_LEFT=100,22
PRIMER_RIGHT=354,21
PRIMER_LEFT_TM=59.635
PRIMER_RIGHT_TM=60.285
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=255
PRIMER_PAIR_PENALTY_1=1.9870
PRIMER_LEFT_1_PENALTY=0.364827
PRIMER_RIGHT_1_PENALTY=1.622131
PRIMER_LEFT_1_SEQUENCE=GCTGCAATATTCCAAACTTCCT
PRIMER_RIGHT_1_SEQUENCE=GTCTGGTGATGACGAGCAGATT
PRIMER_LEFT_1=100,22
PRIMER_RIGHT_1=354,22
PRIMER_LEFT_1_TM=59.635
PRIMER_RIGHT_1_TM=61.622
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=2.0830
PRIMER_LEFT_2_PENALTY=0.797780
PRIMER_RIGHT_2_PENALTY=1.285229
PRIMER_LEFT_2_SEQUENCE=ATTCCAAACTTCCTTCAAAGCC
PRIMER_RIGHT_2_SEQUENCE=GTCTGGTGATGACGAGCAGAT
PRIMER_LEFT_2=108,22
PRIMER_RIGHT_2=354,21
PRIMER_LEFT_2_TM=60.798
PRIMER_RIGHT_2_TM=60.285
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=2.1820
PRIMER_LEFT_3_PENALTY=0.364827
PRIMER_RIGHT_3_PENALTY=1.817214
PRIMER_LEFT_3_SEQUENCE=GCTGCAATATTCCAAACTTCCT
PRIMER_RIGHT_3_SEQUENCE=TCTGGTGATGACGAGCAGATT
PRIMER_LEFT_3=100,22
PRIMER_RIGHT_3=353,21
PRIMER_LEFT_3_TM=59.635
PRIMER_RIGHT_3_TM=60.817
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=2.2953
PRIMER_LEFT_4_PENALTY=0.364827
PRIMER_RIGHT_4_PENALTY=1.930508
PRIMER_LEFT_4_SEQUENCE=GCTGCAATATTCCAAACTTCCT
PRIMER_RIGHT_4_SEQUENCE=GTGAATGTTAGATCAGCCGGT
PRIMER_LEFT_4=100,22
PRIMER_RIGHT_4=215,21
PRIMER_LEFT_4_TM=59.635
PRIMER_RIGHT_4_TM=59.069
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=116
=
PRIMER_SEQUENCE_ID=SSR_01430 Ps_contig_mira-and-tgicl-ass_franssen_13934
SEQUENCE=CGTCTAAAAGTACTAACTTAAACCGGCAATGTTAATAGCCCCAAGAAGTACATACTCTGAAAGAAAGAAAAARCATATAAAGGATGATGAGACCCATTCCAAACATTCTGCTAGGATGCATGTTGTTGCGCGGTAAAACAAACAAGTGCCCATAGGAGTCCTGTGATAAGAAATCCCATTGTGTAGAATAARCTACTGTCTTTAGGCACCACGTACAAACAAGGTTTCTTTGACCACGCGCCAAGCAGCAATGAGATTCCCAAACCAACAAGTGTGTTGAACATAGGACCTGCATAGGATCCAGATAGAGCTATCTGAACACCATCATCTCCATCTAATGCCAATGCAACATTGGACATTAAATCTCCCATTGAATTTCCCCATGCTAATACGGTTAATCCAAGAATCGATGGATTAATTACCAAAAATTAACAACCAAAAGACTAACTAATAGAGMCACAAGCTCATTT
TARGET=59,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01431 Ps_contig_mira-and-tgicl-ass_franssen_13952
SEQUENCE=TACTAGGGAGCTTAGTTAACGAACTTTGAAGCTGGAACAAGGCCTTCTACATGTATTTCTTTTGTCCACCATTTTGATAGATAGAGAATTTCTTTYACTGGACTGAAAATTAATTAATTTTTTAA
TARGET=108,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01432 Ps_contig_mira-and-tgicl-ass_franssen_14004
SEQUENCE=AGGCTTAGAAGGTGCAGTGCCCACAGGCTTTCCCTTCCCCGCACTCACTTTTTTTGCGGGCCGGGCCTAGGTTTTAGGCCCTCATAATCAACTATGCCCGCCCGCCCCCTTTTTTTGTGGGCTTTTGCGGGGCGGGCCTAAACAGGGCGGGTATTGCCCGTTTGCCACCCCTAATTTTGAAATGTATGAATAATTGAAAAGTTTTTGGAATGTTGTGCTCGATTGATTTGATGATAATTGCATTGCACCATTTATTTAAGCTTCTAGCCTTTTATCAACATATATAA
TARGET=96,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1543
PRIMER_LEFT_PENALTY=0.643545
PRIMER_RIGHT_PENALTY=0.510724
PRIMER_LEFT_SEQUENCE=GGTTTTAGGCCCTCATAATCAA
PRIMER_RIGHT_SEQUENCE=CATCAAATCAATCGAGCACAAC
PRIMER_LEFT=69,22
PRIMER_RIGHT=232,22
PRIMER_LEFT_TM=59.356
PRIMER_RIGHT_TM=60.511
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=1.4947
PRIMER_LEFT_1_PENALTY=0.983983
PRIMER_RIGHT_1_PENALTY=0.510724
PRIMER_LEFT_1_SEQUENCE=AGGTTTTAGGCCCTCATAATCA
PRIMER_RIGHT_1_SEQUENCE=CATCAAATCAATCGAGCACAAC
PRIMER_LEFT_1=68,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=59.016
PRIMER_RIGHT_1_TM=60.511
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=1.5996
PRIMER_LEFT_2_PENALTY=1.088859
PRIMER_RIGHT_2_PENALTY=0.510724
PRIMER_LEFT_2_SEQUENCE=GGTTTTAGGCCCTCATAATCAAC
PRIMER_RIGHT_2_SEQUENCE=CATCAAATCAATCGAGCACAAC
PRIMER_LEFT_2=69,23
PRIMER_RIGHT_2=232,22
PRIMER_LEFT_2_TM=60.089
PRIMER_RIGHT_2_TM=60.511
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=1.6894
PRIMER_LEFT_3_PENALTY=0.643545
PRIMER_RIGHT_3_PENALTY=1.045809
PRIMER_LEFT_3_SEQUENCE=GGTTTTAGGCCCTCATAATCAA
PRIMER_RIGHT_3_SEQUENCE=ATCAATCGAGCACAACATTCC
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=226,21
PRIMER_LEFT_3_TM=59.356
PRIMER_RIGHT_3_TM=59.954
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=1.8832
PRIMER_LEFT_4_PENALTY=0.643545
PRIMER_RIGHT_4_PENALTY=1.239704
PRIMER_LEFT_4_SEQUENCE=GGTTTTAGGCCCTCATAATCAA
PRIMER_RIGHT_4_SEQUENCE=AATCAATCGAGCACAACATTCC
PRIMER_LEFT_4=69,22
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=59.356
PRIMER_RIGHT_4_TM=61.240
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_01433 Ps_contig_mira-and-tgicl-ass_franssen_14011
SEQUENCE=GTGATTGAAAGAAGAAGCCTTACCGTCACGAATTCTTCGACGCGGCGATTTCAGGTTCTGATCTTCTTCTTCTTCTGATGTGTGAGGTTGATGATTGGAGTGTGATGAGGG
TARGET=62,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1343
PRIMER_LEFT_PENALTY=0.107763
PRIMER_RIGHT_PENALTY=0.026532
PRIMER_LEFT_SEQUENCE=TGAAAGAAGAAGCCTTACCGTC
PRIMER_RIGHT_SEQUENCE=TCACACTCCAATCATCAACCTC
PRIMER_LEFT=5,22
PRIMER_RIGHT=104,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=59.973
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=0.3482
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.240472
PRIMER_LEFT_1_SEQUENCE=TGAAAGAAGAAGCCTTACCGTC
PRIMER_RIGHT_1_SEQUENCE=CATCACACTCCAATCATCAACC
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=106,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.240
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=0.3482
PRIMER_LEFT_2_PENALTY=0.107763
PRIMER_RIGHT_2_PENALTY=0.240472
PRIMER_LEFT_2_SEQUENCE=GAAAGAAGAAGCCTTACCGTCA
PRIMER_RIGHT_2_SEQUENCE=CATCACACTCCAATCATCAACC
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=106,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=60.240
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=101
PRIMER_PAIR_PENALTY_3=0.5939
PRIMER_LEFT_3_PENALTY=0.567360
PRIMER_RIGHT_3_PENALTY=0.026532
PRIMER_LEFT_3_SEQUENCE=TTGAAAGAAGAAGCCTTACCGT
PRIMER_RIGHT_3_SEQUENCE=TCACACTCCAATCATCAACCTC
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=104,22
PRIMER_LEFT_3_TM=59.433
PRIMER_RIGHT_3_TM=59.973
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=0.8078
PRIMER_LEFT_4_PENALTY=0.567360
PRIMER_RIGHT_4_PENALTY=0.240472
PRIMER_LEFT_4_SEQUENCE=TTGAAAGAAGAAGCCTTACCGT
PRIMER_RIGHT_4_SEQUENCE=CATCACACTCCAATCATCAACC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=106,22
PRIMER_LEFT_4_TM=59.433
PRIMER_RIGHT_4_TM=60.240
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_01434 Ps_contig_mira-and-tgicl-ass_franssen_14016
SEQUENCE=AGGCACATCCTTGCATTGCAGACATGTTTGGGAGAGACTTCTCACTTTGGTTCCTGACTGAGACAGGATTGCTGATCGTCGACCGCATCGGTACTCAACCAGACTYAATCATCAGAGGATTATGGACCAGGTTCAAGACAGAATTGGCCGAGATGAGGGCAAACATGGCCCAGTTCATGATGATGATGCAAGGAGTTGTTCAGGGGCAAGAGGAGCTGCGTGCCTTAGCCTAGAGACTGGAAGCCGTGATTCCACCAGTCCACCGTGCTTCA
TARGET=177,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01435 Ps_contig_mira-and-tgicl-ass_franssen_14046
SEQUENCE=TTGGAAATCGACGGCGCAGATTATCTCACACGGTAGGGATTCTCTCTTAGCCCCTGATTCTGTATTCTGATTCAAGCCATAACGGTAACAAGAGAATATTGAGGAGTAGTAGTAGTGCTCAGGGTTTGGATTTGAAATATAGTAGATTTGATGTATTAATGCGTGGT
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.4182
PRIMER_LEFT_PENALTY=0.061624
PRIMER_RIGHT_PENALTY=4.356567
PRIMER_LEFT_SEQUENCE=GGATTCTCTCTTAGCCCCTGAT
PRIMER_RIGHT_SEQUENCE=TTTCAAATCCAAACCCTGAG
PRIMER_LEFT=36,22
PRIMER_RIGHT=136,20
PRIMER_LEFT_TM=60.062
PRIMER_RIGHT_TM=57.643
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=4.4883
PRIMER_LEFT_1_PENALTY=0.131699
PRIMER_RIGHT_1_PENALTY=4.356567
PRIMER_LEFT_1_SEQUENCE=TCACACGGTAGGGATTCTCTCT
PRIMER_RIGHT_1_SEQUENCE=TTTCAAATCCAAACCCTGAG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=136,20
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=57.643
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=112
PRIMER_PAIR_PENALTY_2=4.4883
PRIMER_LEFT_2_PENALTY=0.131699
PRIMER_RIGHT_2_PENALTY=4.356567
PRIMER_LEFT_2_SEQUENCE=CTCACACGGTAGGGATTCTCTC
PRIMER_RIGHT_2_SEQUENCE=TTTCAAATCCAAACCCTGAG
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=136,20
PRIMER_LEFT_2_TM=60.132
PRIMER_RIGHT_2_TM=57.643
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=4.5658
PRIMER_LEFT_3_PENALTY=0.209271
PRIMER_RIGHT_3_PENALTY=4.356567
PRIMER_LEFT_3_SEQUENCE=CGCAGATTATCTCACACGGTAG
PRIMER_RIGHT_3_SEQUENCE=TTTCAAATCCAAACCCTGAG
PRIMER_LEFT_3=14,22
PRIMER_RIGHT_3=136,20
PRIMER_LEFT_3_TM=59.791
PRIMER_RIGHT_3_TM=57.643
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=123
PRIMER_PAIR_PENALTY_4=4.6356
PRIMER_LEFT_4_PENALTY=0.279044
PRIMER_RIGHT_4_PENALTY=4.356567
PRIMER_LEFT_4_SEQUENCE=TAGGGATTCTCTCTTAGCCCCT
PRIMER_RIGHT_4_SEQUENCE=TTTCAAATCCAAACCCTGAG
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=136,20
PRIMER_LEFT_4_TM=59.721
PRIMER_RIGHT_4_TM=57.643
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=104
=
PRIMER_SEQUENCE_ID=SSR_01436 Ps_contig_mira-and-tgicl-ass_franssen_14049
SEQUENCE=GAAGCAAGCATTGAAGAAATACACTCGGATGATATATTGTTAGAAAGAGATGTTCCACCACCTTTATGTGAGAAAGACAAAGTGGAGAAAGCAAGGCGAAGTGAGTTTGTACAAGGGCTTTTGTTTTCTACTATTCTTGATCCTGACTTTTGACCCATACATTATCGGTTTTTATCAATTTGATGGATGGAAAAAGAGACGAAAAGAAGGCTACGTCTTCTTGAGATGATAAATAGTATGTGTATGAATTATACGTATGCACTGGGATGTATCTAGTCAATAGTATATAGAGCTAAGCTATTCAATAAAGAGAGAGAGAGAGAGCGCTAAGCTATGTAGTAAGAAATTATTATTTTATTTATTTGTAATGTAAATTCAAGTCTATGAAATTGGTATTTAG
TARGET=309,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01437 Ps_contig_mira-and-tgicl-ass_franssen_14054
SEQUENCE=CGAGGGGTGGCGGTCCGTATGGAACCGAAATAGGTCGAAGAAATAGTACAAAGAAAGAAGTTTGGTCGATTCCGAGACAAGCAGCAATCTTTTATTCAGAAAACAACACCGAAAGCTCCAAGAGACGAAGATCTTCGAGTGTGGGATGATAAAAGAGATGAGTCTCATGACAAGTTAATAACCATTGATAACGTTCAACAACAACAAGGTCAAATCGCAGGGTTGAAGCTGGATCATGCATCCTCCTCGACCACCACGGGGACAGAATTGGAAAGAGGGAATCAAAGTAATGATCATTGGACAAATGTGATGGATTCTAGAATCGGGAAAATGCAGGGTGATCAACAAAGGTTTAACATGAATAATAGTCCGTATTCAAATGCACCTATCTCGATAAACCAAAATGGCGAAAACTGCATAATGGATTCTACTCCTACTACTTATGATGATTTATCCGAGTTGAGTAACTTCGCTGTTGGTGGACATGTCTCGCTTGCTTTGGAGTTGAGGAACTCGGAAAGTGATGGATTTGGTTTGTCGAATGATGACGTCAATAAAAGGCGTAATCAAACATTGGCTTCTTCGCCGGATAATGATTTGTTAGATTATCATTTCACAGACAATGGAAAGCAACAACATAAGTTTGGCAATCCTCATTTATTACACGAGTTTGTTGTCTGAATTGAAAGAAGTTCGTGTTTGGATAGCATCACATTTGAAGTTCTCCGATTGTCTGCAGTTAACAATCATACTCATTCACTCAGTCAGGTCATCGATGATCGTTGTATCGAGATCAGACAGTTCAAATTTCAAAGCAAAATTTGATTTTCTTAAAACTATATCTTGATCTCAATTCGACGACCACTGATGTACTGACTGCGTGAATGTCTGAGAATGCTGACTATAGGTAATCCAAATTCGTTATATTCRCGGTNTTACAACNCGTCTAT
TARGET=196,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01438 Ps_contig_mira-and-tgicl-ass_franssen_14055
SEQUENCE=GAGAATGCTGACTATAGGTAATCCAAATTCGTTATATTCACGGTGTTACAACACGTCTATCTCAAAGCTACAAACCTACAACTACTAGCTTCACGCCGTGCGTCTTAATACATCGGCGTCTTGATACATTTCCAAACACATTGGAATATTTTTAATGTTTTGTTGAGGGAATTATGGAAAATGTGTTCCTTAAAAAAAAGTGAAACTAGGAAGAGAAAAAACATATGGTGTTACTTTGTGCTATTACTCATTATTGTTGTTGTTGATAGTGAATGATAAAGGTGTATGTAAAGCATGAACCGTACAAAATATACGAGTAGGTATGTGTAGATATGTTGTAATATTGTGGGGGTGATTGATTGTATGTATTTGCAGGCAATTTTGTAAGGACAAAGAAAGAGCATGTCCAATGCATCATCATCATAAATAAGGAAAGAATCTTTGTGAAAA
TARGET=254,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4071
PRIMER_LEFT_PENALTY=0.381973
PRIMER_RIGHT_PENALTY=0.025128
PRIMER_LEFT_SEQUENCE=TACAACTACTAGCTTCACGCCG
PRIMER_RIGHT_SEQUENCE=TCACCCCCACAATATTACAACA
PRIMER_LEFT=76,22
PRIMER_RIGHT=354,22
PRIMER_LEFT_TM=59.618
PRIMER_RIGHT_TM=59.975
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=13.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.4439
PRIMER_LEFT_1_PENALTY=0.381973
PRIMER_RIGHT_1_PENALTY=0.061961
PRIMER_LEFT_1_SEQUENCE=TACAACTACTAGCTTCACGCCG
PRIMER_RIGHT_1_SEQUENCE=TACATACAATCAATCACCCCCA
PRIMER_LEFT_1=76,22
PRIMER_RIGHT_1=367,22
PRIMER_LEFT_1_TM=59.618
PRIMER_RIGHT_1_TM=59.938
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=13.4000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.5240
PRIMER_LEFT_2_PENALTY=0.498888
PRIMER_RIGHT_2_PENALTY=0.025128
PRIMER_LEFT_2_SEQUENCE=AATACATCGGCGTCTTGATACA
PRIMER_RIGHT_2_SEQUENCE=TCACCCCCACAATATTACAACA
PRIMER_LEFT_2=106,22
PRIMER_RIGHT_2=354,22
PRIMER_LEFT_2_TM=59.501
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.5608
PRIMER_LEFT_3_PENALTY=0.498888
PRIMER_RIGHT_3_PENALTY=0.061961
PRIMER_LEFT_3_SEQUENCE=TACATCGGCGTCTTGATACATT
PRIMER_RIGHT_3_SEQUENCE=TACATACAATCAATCACCCCCA
PRIMER_LEFT_3=108,22
PRIMER_RIGHT_3=367,22
PRIMER_LEFT_3_TM=59.501
PRIMER_RIGHT_3_TM=59.938
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.5608
PRIMER_LEFT_4_PENALTY=0.498888
PRIMER_RIGHT_4_PENALTY=0.061961
PRIMER_LEFT_4_SEQUENCE=AATACATCGGCGTCTTGATACA
PRIMER_RIGHT_4_SEQUENCE=TACATACAATCAATCACCCCCA
PRIMER_LEFT_4=106,22
PRIMER_RIGHT_4=367,22
PRIMER_LEFT_4_TM=59.501
PRIMER_RIGHT_4_TM=59.938
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_01439 Ps_contig_mira-and-tgicl-ass_franssen_14055
SEQUENCE=GAGAATGCTGACTATAGGTAATCCAAATTCGTTATATTCACGGTGTTACAACACGTCTATCTCAAAGCTACAAACCTACAACTACTAGCTTCACGCCGTGCGTCTTAATACATCGGCGTCTTGATACATTTCCAAACACATTGGAATATTTTTAATGTTTTGTTGAGGGAATTATGGAAAATGTGTTCCTTAAAAAAAAGTGAAACTAGGAAGAGAAAAAACATATGGTGTTACTTTGTGCTATTACTCATTATTGTTGTTGTTGATAGTGAATGATAAAGGTGTATGTAAAGCATGAACCGTACAAAATATACGAGTAGGTATGTGTAGATATGTTGTAATATTGTGGGGGTGATTGATTGTATGTATTTGCAGGCAATTTTGTAAGGACAAAGAAAGAGCATGTCCAATGCATCATCATCATAAATAAGGAAAGAATCTTTGTGAAAA
TARGET=413,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01440 Ps_contig_mira-and-tgicl-ass_franssen_14061
SEQUENCE=GGCGTCACAATCTCTCAAGACTCTTCTCTCCACCTATTTCTTCCCCACTCCCTCATTTTCGTCCCTGACAGACACTCACACTCCACTCCCCAACGTCTCTCTCCACTGCTAACAACCTCACGCTCTCTCTCTCTAAAAGACCCTCACCTTCCACTCATTTTTACGGACAACCCACAAACCCTTCTTTCACTCTCTCTCACGCAAAAGAGAAAACAGAAAGAGAAGGCGGAAGAGAAGAAGAAAAACCCTTCTTGTTCTCCAACCCCGCCGCAAACCTCCGAACCCTTTCTCCTCTCACTGACCAAAAACCCATCACAACCTCCGTCTCCACTACCTTCAAAACACAAGCTCAGCCGCCGTCAACCTCCACTGCCGCCGCGGATTCACCACCGCGAAACCATCGACCACCCAAAACCCACCGTAAACCTTCAAAATCCCAATCGTCAAACCCTTCTTCATCACAACACWTTAAAAAAAAAATGCTTATCCAAATAAGCAACTTTAAAAACTAGAAAATAAAGGATGGAAACAAACAGATTAA
TARGET=123,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01441 Ps_contig_mira-and-tgicl-ass_franssen_14066
SEQUENCE=CGGCGAACATGGTGTATGGTAGAGTAAAAAGGACGACGACGGAAGGTTGTGTGTGTGAGTTTGGTTTATGTGGTTGACGTTGTAGGGCGGTCGAAGGTGGTTTCGTTGGTGGCTTGTTCGGTTGACCGCCGTTTTATCGTGTTGCGTTTGAAATGGTGGCGACGGCGTCGGTAAAAAAGCAGGAGGTGCAGCGGTTCGGACTAGTAAAACCCCGCTTCCTCTTTCTGTCTTCTTTGTGTTTTTGTTTGTGAGATTTTCAATGAATAGTTGGCTTTTCAAGTAGAAAAAAACAAGGTTGTTCTCGAGGTTAAGGAGGAGATGAA
TARGET=48,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6603
PRIMER_LEFT_PENALTY=0.478252
PRIMER_RIGHT_PENALTY=0.182061
PRIMER_LEFT_SEQUENCE=GCGAACATGGTGTATGGTAGAG
PRIMER_RIGHT_SEQUENCE=GTTTTACTAGTCCGAACCGCTG
PRIMER_LEFT=2,22
PRIMER_RIGHT=209,22
PRIMER_LEFT_TM=59.522
PRIMER_RIGHT_TM=60.182
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.9429
PRIMER_LEFT_1_PENALTY=0.478252
PRIMER_RIGHT_1_PENALTY=0.464628
PRIMER_LEFT_1_SEQUENCE=GCGAACATGGTGTATGGTAGAG
PRIMER_RIGHT_1_SEQUENCE=AAGAGGAAGCGGGGTTTTACTA
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=222,22
PRIMER_LEFT_1_TM=59.522
PRIMER_RIGHT_1_TM=60.465
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=4.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=1.1780
PRIMER_LEFT_2_PENALTY=0.478252
PRIMER_RIGHT_2_PENALTY=0.699731
PRIMER_LEFT_2_SEQUENCE=GCGAACATGGTGTATGGTAGAG
PRIMER_RIGHT_2_SEQUENCE=AAGAAGACAGAAAGAGGAAGCG
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=233,22
PRIMER_LEFT_2_TM=59.522
PRIMER_RIGHT_2_TM=59.300
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=1.3387
PRIMER_LEFT_3_PENALTY=0.478252
PRIMER_RIGHT_3_PENALTY=0.860451
PRIMER_LEFT_3_SEQUENCE=GCGAACATGGTGTATGGTAGAG
PRIMER_RIGHT_3_SEQUENCE=GGTTTTACTAGTCCGAACCGCT
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=210,22
PRIMER_LEFT_3_TM=59.522
PRIMER_RIGHT_3_TM=60.860
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=1.3507
PRIMER_LEFT_4_PENALTY=1.168677
PRIMER_RIGHT_4_PENALTY=0.182061
PRIMER_LEFT_4_SEQUENCE=TGGTAGAGTAAAAAGGACGACGA
PRIMER_RIGHT_4_SEQUENCE=GTTTTACTAGTCCGAACCGCTG
PRIMER_LEFT_4=16,23
PRIMER_RIGHT_4=209,22
PRIMER_LEFT_4_TM=60.169
PRIMER_RIGHT_4_TM=60.182
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_01442 Ps_contig_mira-and-tgicl-ass_franssen_14072
SEQUENCE=TTTGTCAACAGAAGCAACCCTGGAGAAGGATAATTATTTTCTTTATCAACCATCCAAGTTTACAATCCAATAAAATATATATATATATAAAAACATGGCCAAGAAAAAAAAACTAATCTAAAATTTAAACAGGCACCCATCTCTAGTTCAACTTTTCTACATTCACAAAGGCACGAATATATTGAGGTAATAGCCCCAAAAGAATGTGCTGTAAACTCTAGTAAATTGTAACCGACCCTTAGGATATTGTTATACCGCGGGTAAAATGACAAAACTCAGAGCCCTACAAAACCACAACACCAGGAAACAGAAACAACCAGTCCGCAGAATCCATTATGGCTTGTTGAGGAAACCTCGTATAAATATCCCAGAAGCTGGAACCGACTGTCTTGATGGGACCTAGTGATGTGGTGCAAGCACAGAGACAAAATTTTGATTTTCACACCACCCCAACCAATTTGCTTTTAGGCTGACAGGGCTCCTTCATTATTCGTACCCCACTCTTCGTCAGCCTCGCTGCCGTTTACAAGTACTGCACCATCTGTTGATTCGGCATCCACTACAACTGCCTCTTCTTGGCTTCCATCCTCTCCATTTTGTTCCTCCGTGTAGTGTTCTTCCGTAAAGTGCTCTTCATTTTGTTCAGTCAACTCATGATTAGAATCTCCATTCTCAAGACGCTCCTCCTCTTCAATCTGCATGTTTCTGAAAGCTTCAACAAGGGTAACATGTGGTTTGTCAGCATGGTTGATGTACTGCTCTGCTGGAGGGTAAACATTCAATGTTTCTGCAGCTTTAGACTCAACAGCAAGTGCAACTTGCCAGTCTTCAAACAGATTTGGATATTCCTCAGGATCAGCCAATGATTCAGCAGCTTTTGGATTAACCTTATTGAGATCCTTTCTCCAAATTGCCACTATCTCTGGGACCTTACTTGGGAGATAAGATCGAGCCATTAAAGCTGCCTCTGGAATTCGATTGCTTTCTACCAATAGTTGAAGGCAGTCTTCAAGTTTACCCAACATAAATAAGCAAAGGAATGCAACATTGTTCTTCCCTTGCTCCTTAGCAAGGGTTGCAAGTTTTGAAATTCCTTCAGCATCTACCGAAAGAAGAATATAGCAAATAACAACCCACTCAAAATCCATTGCATGATTACAAACATTCCTCAGCCTATTTTGTACTTGCCAACTAGACATAGCTTAACTTTCCTACCCCAACCTGGTCCTTTACCCTAAGTTTTAAGTACTTCCTA
TARGET=75,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3996
PRIMER_LEFT_PENALTY=0.328860
PRIMER_RIGHT_PENALTY=0.070748
PRIMER_LEFT_SEQUENCE=AGCAACCCTGGAGAAGGATAAT
PRIMER_RIGHT_SEQUENCE=TCCTGGTGTTGTGGTTTTGTAG
PRIMER_LEFT=12,22
PRIMER_RIGHT=304,22
PRIMER_LEFT_TM=60.329
PRIMER_RIGHT_TM=59.929
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.8000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.4152
PRIMER_LEFT_1_PENALTY=0.328860
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=AGCAACCCTGGAGAAGGATAAT
PRIMER_RIGHT_1_SEQUENCE=TGTAGGGCTCTGAGTTTTGTCA
PRIMER_LEFT_1=12,22
PRIMER_RIGHT_1=287,22
PRIMER_LEFT_1_TM=60.329
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.4934
PRIMER_LEFT_2_PENALTY=0.328860
PRIMER_RIGHT_2_PENALTY=0.164560
PRIMER_LEFT_2_SEQUENCE=AGCAACCCTGGAGAAGGATAAT
PRIMER_RIGHT_2_SEQUENCE=TGGTTTTGTAGGGCTCTGAGTT
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=60.329
PRIMER_RIGHT_2_TM=60.165
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.6257
PRIMER_LEFT_3_PENALTY=0.328860
PRIMER_RIGHT_3_PENALTY=0.296849
PRIMER_LEFT_3_SEQUENCE=AGCAACCCTGGAGAAGGATAAT
PRIMER_RIGHT_3_SEQUENCE=TTCCTGGTGTTGTGGTTTTGTA
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=305,22
PRIMER_LEFT_3_TM=60.329
PRIMER_RIGHT_3_TM=60.297
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=0.6466
PRIMER_LEFT_4_PENALTY=0.328860
PRIMER_RIGHT_4_PENALTY=0.317768
PRIMER_LEFT_4_SEQUENCE=AGCAACCCTGGAGAAGGATAAT
PRIMER_RIGHT_4_SEQUENCE=GTGGTTTTGTAGGGCTCTGAGT
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=60.329
PRIMER_RIGHT_4_TM=59.682
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_01443 Ps_contig_mira-and-tgicl-ass_franssen_14080
SEQUENCE=GTTAACACCGTGATACACTCTCTTTTCATCTTCCTAACAAACAACAACGCAACACCGCAACCGTGTCATCTTTGAAAAGAAATTAACAATTTCAGTTTCGGCTTCACATCGTCACACTGCCTAAAGATCGATTACAAGGGGGTTTCAAGTCTGAAGGTTTGTGGAAGATCATGGTGTGCTCTTTGGACCGGTGAAAGAAGGAGTCAAGTGGTAGCCAAAATCACCAGATCCAGCCAACAATAGAGGTGACGCGGGAAAGGCCGGAAGTTCTATTTTCAACTCGGTAAAAGTAGGTTGTTGAAGACAAGCATCATCACTAAAAGTAAATCAATTCCGGTCAACGTTATACAGATTGGGTAATATATATATATCCTGATTTCATATGAATATTGTTTGGTTTACCCAGTTTCCTTCGTCAAAAATGCTTGTGAGATAATTATAATAGAAACTTGTGTTCATGTGGTACTATTGGATTGTTGCTGCCGTATCAATTTGAAAAAATAATAATTAGCTGCTATATGATATATCTGCATTTTTTATCACGAAACTTTACTGTGGAGATCCTATTTTTGAGGAGCCTATATTGATTAGTTTGGGTTGATAAAATAATTCGTGATGGTATTATTTGTGAGGAATATTTGGTATAATTGTTCTTTGTGGAAAGAGCTTGTAGAAAGTTGGTGAAGAATGCTAGGCTATTTGTTTAGAACCTCCCACCCCGAGGTACAAAGGTTCTTG
TARGET=360,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1053
PRIMER_LEFT_PENALTY=0.024937
PRIMER_RIGHT_PENALTY=0.080323
PRIMER_LEFT_SEQUENCE=GAAAGGCCGGAAGTTCTATTTT
PRIMER_RIGHT_SEQUENCE=CTCACAAGCATTTTTGACGAAG
PRIMER_LEFT=254,22
PRIMER_RIGHT=431,22
PRIMER_LEFT_TM=59.975
PRIMER_RIGHT_TM=59.920
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.1053
PRIMER_LEFT_1_PENALTY=0.024937
PRIMER_RIGHT_1_PENALTY=0.080323
PRIMER_LEFT_1_SEQUENCE=AAAGGCCGGAAGTTCTATTTTC
PRIMER_RIGHT_1_SEQUENCE=CTCACAAGCATTTTTGACGAAG
PRIMER_LEFT_1=255,22
PRIMER_RIGHT_1=431,22
PRIMER_LEFT_1_TM=59.975
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.1472
PRIMER_LEFT_2_PENALTY=0.024937
PRIMER_RIGHT_2_PENALTY=0.122257
PRIMER_LEFT_2_SEQUENCE=GAAAGGCCGGAAGTTCTATTTT
PRIMER_RIGHT_2_SEQUENCE=GGAAACTGGGTAAACCAAACAA
PRIMER_LEFT_2=254,22
PRIMER_RIGHT_2=410,22
PRIMER_LEFT_2_TM=59.975
PRIMER_RIGHT_2_TM=60.122
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.1472
PRIMER_LEFT_3_PENALTY=0.024937
PRIMER_RIGHT_3_PENALTY=0.122257
PRIMER_LEFT_3_SEQUENCE=AAAGGCCGGAAGTTCTATTTTC
PRIMER_RIGHT_3_SEQUENCE=GGAAACTGGGTAAACCAAACAA
PRIMER_LEFT_3=255,22
PRIMER_RIGHT_3=410,22
PRIMER_LEFT_3_TM=59.975
PRIMER_RIGHT_3_TM=60.122
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.1980
PRIMER_LEFT_4_PENALTY=0.075753
PRIMER_RIGHT_4_PENALTY=0.122257
PRIMER_LEFT_4_SEQUENCE=AAAGATCGATTACAAGGGGGTT
PRIMER_RIGHT_4_SEQUENCE=GGAAACTGGGTAAACCAAACAA
PRIMER_LEFT_4=122,22
PRIMER_RIGHT_4=410,22
PRIMER_LEFT_4_TM=60.076
PRIMER_RIGHT_4_TM=60.122
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_01444 Ps_contig_mira-and-tgicl-ass_franssen_14096
SEQUENCE=GATGATATTTATTTATTTTGGGTGATTCACTTTCAACGAGTTCCACCGATATTTAGTTTTTCCGGTGACACAAAAAATAAAAGGCTTAAAAAATTTTACAGCTTCAAACATTTTGCGATGGCTCTACTCTCCTCCACACTACGCGCTCCACTCGTCTTCTCCAAAAACCCTAAACCCGTCTCTCTCTCTTCCCTTCACTCTCGAATCTATCTATCTCCTCGCTCTCCCAGGTTCCCTTCCCTTCGCTTCATCTCCGCCGCCGGCGATACCGGAGACGCCGAAAAGCCATCTTCCAATATCTCCGACGAGTGGGGCGAGGGGTCTGAGCCCGAAACCAAACCGTTCACATATTTCAAGCTTCCAGATTCGGATCCTCCCAAGGACGAGGATGAATGGGGAAAAGGAGCGGCAGCTGGCGCCGGAAGCTATAACGACGCCGGGAATGGAACTCCAACTTTCGCTGCCGAAGCTTCGCCGGAGGCGGAGGCGGAGGATGGAGTTGATGAAAATCTTGAGGGGCTGAAGCGTTCTCTTGTTGACACTGTGTACGGGACTGAGCTCGGGTTCCGAGCTCGGTCAGAGGTTCGAGCTGAGGTGTCCGAGTTTGTGGCTCAATTGGAAGCTGCTAACCCCACCCCCGCTCCGGTTGAGGAACCTGACCTTCTCAATGGGAATTGGGTGCTGCTGTACACAGCATCTTCTGAACTGTTGCCACTTTTAGCAGCAGGATCATTGCCTTTGTTGAAGTTGGATAAGATTTCGCAAACAATTGATACGGATAGCTTCACTGTTGTAAACTCTACGACATTGTCTAGCCCTTTTGCCTCGTTTTCTTTTAGTGTATCTGCTTCATTTGAAGTTCGAAGCCCTACAAGAATACAGGTTACATTCAAAGAAGGATCACTACAACCTCCAGAAATAAAGTCTAAAATTGACCTACCAGAAAACATAAACATTTTTGGTCAACAACTTAGCCTAGGGCCTCTGCTACAGTCTCTCGGACCCCTGGAGAATGTGGTGGCAAACATATCACGTGTCATTTCAGGTCAGTCACCTCTTAAGATTCCCATACCGGGTGAGAGGACAAGCTCCTGGCTTATTACCACATATCTTGATAAGGACTTGCGAATATCAAGGGGAGATGGTGGTTTGTTTGTTCTTGCAAGAGAAGGGAGTTCCCTTCTTGATCAGTAAGAAAATAATAATCTTTTCATTTTGATACTCCACCACCCCGTTTCCCTCTGCCCACCCAAAATGTGAAAATTCATATACATTTTTTACGTTCTCGAGATGTTATATGTTGTATATTCAAAGGATACAGAGCTTATGTTGTCCACATGTAATTTTACTATATATATAAAATGTTGGAACGGAATGTTCCCCTATTAACTTAGGTAAAWATTGATTTGGGGGGAAGGAATGTGAAATCAAATTTATAAAG
TARGET=6,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01445 Ps_contig_mira-and-tgicl-ass_franssen_14096
SEQUENCE=GATGATATTTATTTATTTTGGGTGATTCACTTTCAACGAGTTCCACCGATATTTAGTTTTTCCGGTGACACAAAAAATAAAAGGCTTAAAAAATTTTACAGCTTCAAACATTTTGCGATGGCTCTACTCTCCTCCACACTACGCGCTCCACTCGTCTTCTCCAAAAACCCTAAACCCGTCTCTCTCTCTTCCCTTCACTCTCGAATCTATCTATCTCCTCGCTCTCCCAGGTTCCCTTCCCTTCGCTTCATCTCCGCCGCCGGCGATACCGGAGACGCCGAAAAGCCATCTTCCAATATCTCCGACGAGTGGGGCGAGGGGTCTGAGCCCGAAACCAAACCGTTCACATATTTCAAGCTTCCAGATTCGGATCCTCCCAAGGACGAGGATGAATGGGGAAAAGGAGCGGCAGCTGGCGCCGGAAGCTATAACGACGCCGGGAATGGAACTCCAACTTTCGCTGCCGAAGCTTCGCCGGAGGCGGAGGCGGAGGATGGAGTTGATGAAAATCTTGAGGGGCTGAAGCGTTCTCTTGTTGACACTGTGTACGGGACTGAGCTCGGGTTCCGAGCTCGGTCAGAGGTTCGAGCTGAGGTGTCCGAGTTTGTGGCTCAATTGGAAGCTGCTAACCCCACCCCCGCTCCGGTTGAGGAACCTGACCTTCTCAATGGGAATTGGGTGCTGCTGTACACAGCATCTTCTGAACTGTTGCCACTTTTAGCAGCAGGATCATTGCCTTTGTTGAAGTTGGATAAGATTTCGCAAACAATTGATACGGATAGCTTCACTGTTGTAAACTCTACGACATTGTCTAGCCCTTTTGCCTCGTTTTCTTTTAGTGTATCTGCTTCATTTGAAGTTCGAAGCCCTACAAGAATACAGGTTACATTCAAAGAAGGATCACTACAACCTCCAGAAATAAAGTCTAAAATTGACCTACCAGAAAACATAAACATTTTTGGTCAACAACTTAGCCTAGGGCCTCTGCTACAGTCTCTCGGACCCCTGGAGAATGTGGTGGCAAACATATCACGTGTCATTTCAGGTCAGTCACCTCTTAAGATTCCCATACCGGGTGAGAGGACAAGCTCCTGGCTTATTACCACATATCTTGATAAGGACTTGCGAATATCAAGGGGAGATGGTGGTTTGTTTGTTCTTGCAAGAGAAGGGAGTTCCCTTCTTGATCAGTAAGAAAATAATAATCTTTTCATTTTGATACTCCACCACCCCGTTTCCCTCTGCCCACCCAAAATGTGAAAATTCATATACATTTTTTACGTTCTCGAGATGTTATATGTTGTATATTCAAAGGATACAGAGCTTATGTTGTCCACATGTAATTTTACTATATATATAAAATGTTGGAACGGAATGTTCCCCTATTAACTTAGGTAAAWATTGATTTGGGGGGAAGGAATGTGAAATCAAATTTATAAAG
TARGET=179,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01446 Ps_contig_mira-and-tgicl-ass_franssen_14096
SEQUENCE=GATGATATTTATTTATTTTGGGTGATTCACTTTCAACGAGTTCCACCGATATTTAGTTTTTCCGGTGACACAAAAAATAAAAGGCTTAAAAAATTTTACAGCTTCAAACATTTTGCGATGGCTCTACTCTCCTCCACACTACGCGCTCCACTCGTCTTCTCCAAAAACCCTAAACCCGTCTCTCTCTCTTCCCTTCACTCTCGAATCTATCTATCTCCTCGCTCTCCCAGGTTCCCTTCCCTTCGCTTCATCTCCGCCGCCGGCGATACCGGAGACGCCGAAAAGCCATCTTCCAATATCTCCGACGAGTGGGGCGAGGGGTCTGAGCCCGAAACCAAACCGTTCACATATTTCAAGCTTCCAGATTCGGATCCTCCCAAGGACGAGGATGAATGGGGAAAAGGAGCGGCAGCTGGCGCCGGAAGCTATAACGACGCCGGGAATGGAACTCCAACTTTCGCTGCCGAAGCTTCGCCGGAGGCGGAGGCGGAGGATGGAGTTGATGAAAATCTTGAGGGGCTGAAGCGTTCTCTTGTTGACACTGTGTACGGGACTGAGCTCGGGTTCCGAGCTCGGTCAGAGGTTCGAGCTGAGGTGTCCGAGTTTGTGGCTCAATTGGAAGCTGCTAACCCCACCCCCGCTCCGGTTGAGGAACCTGACCTTCTCAATGGGAATTGGGTGCTGCTGTACACAGCATCTTCTGAACTGTTGCCACTTTTAGCAGCAGGATCATTGCCTTTGTTGAAGTTGGATAAGATTTCGCAAACAATTGATACGGATAGCTTCACTGTTGTAAACTCTACGACATTGTCTAGCCCTTTTGCCTCGTTTTCTTTTAGTGTATCTGCTTCATTTGAAGTTCGAAGCCCTACAAGAATACAGGTTACATTCAAAGAAGGATCACTACAACCTCCAGAAATAAAGTCTAAAATTGACCTACCAGAAAACATAAACATTTTTGGTCAACAACTTAGCCTAGGGCCTCTGCTACAGTCTCTCGGACCCCTGGAGAATGTGGTGGCAAACATATCACGTGTCATTTCAGGTCAGTCACCTCTTAAGATTCCCATACCGGGTGAGAGGACAAGCTCCTGGCTTATTACCACATATCTTGATAAGGACTTGCGAATATCAAGGGGAGATGGTGGTTTGTTTGTTCTTGCAAGAGAAGGGAGTTCCCTTCTTGATCAGTAAGAAAATAATAATCTTTTCATTTTGATACTCCACCACCCCGTTTCCCTCTGCCCACCCAAAATGTGAAAATTCATATACATTTTTTACGTTCTCGAGATGTTATATGTTGTATATTCAAAGGATACAGAGCTTATGTTGTCCACATGTAATTTTACTATATATATAAAATGTTGGAACGGAATGTTCCCCTATTAACTTAGGTAAAWATTGATTTGGGGGGAAGGAATGTGAAATCAAATTTATAAAG
TARGET=205,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01447 Ps_contig_mira-and-tgicl-ass_franssen_14096
SEQUENCE=GATGATATTTATTTATTTTGGGTGATTCACTTTCAACGAGTTCCACCGATATTTAGTTTTTCCGGTGACACAAAAAATAAAAGGCTTAAAAAATTTTACAGCTTCAAACATTTTGCGATGGCTCTACTCTCCTCCACACTACGCGCTCCACTCGTCTTCTCCAAAAACCCTAAACCCGTCTCTCTCTCTTCCCTTCACTCTCGAATCTATCTATCTCCTCGCTCTCCCAGGTTCCCTTCCCTTCGCTTCATCTCCGCCGCCGGCGATACCGGAGACGCCGAAAAGCCATCTTCCAATATCTCCGACGAGTGGGGCGAGGGGTCTGAGCCCGAAACCAAACCGTTCACATATTTCAAGCTTCCAGATTCGGATCCTCCCAAGGACGAGGATGAATGGGGAAAAGGAGCGGCAGCTGGCGCCGGAAGCTATAACGACGCCGGGAATGGAACTCCAACTTTCGCTGCCGAAGCTTCGCCGGAGGCGGAGGCGGAGGATGGAGTTGATGAAAATCTTGAGGGGCTGAAGCGTTCTCTTGTTGACACTGTGTACGGGACTGAGCTCGGGTTCCGAGCTCGGTCAGAGGTTCGAGCTGAGGTGTCCGAGTTTGTGGCTCAATTGGAAGCTGCTAACCCCACCCCCGCTCCGGTTGAGGAACCTGACCTTCTCAATGGGAATTGGGTGCTGCTGTACACAGCATCTTCTGAACTGTTGCCACTTTTAGCAGCAGGATCATTGCCTTTGTTGAAGTTGGATAAGATTTCGCAAACAATTGATACGGATAGCTTCACTGTTGTAAACTCTACGACATTGTCTAGCCCTTTTGCCTCGTTTTCTTTTAGTGTATCTGCTTCATTTGAAGTTCGAAGCCCTACAAGAATACAGGTTACATTCAAAGAAGGATCACTACAACCTCCAGAAATAAAGTCTAAAATTGACCTACCAGAAAACATAAACATTTTTGGTCAACAACTTAGCCTAGGGCCTCTGCTACAGTCTCTCGGACCCCTGGAGAATGTGGTGGCAAACATATCACGTGTCATTTCAGGTCAGTCACCTCTTAAGATTCCCATACCGGGTGAGAGGACAAGCTCCTGGCTTATTACCACATATCTTGATAAGGACTTGCGAATATCAAGGGGAGATGGTGGTTTGTTTGTTCTTGCAAGAGAAGGGAGTTCCCTTCTTGATCAGTAAGAAAATAATAATCTTTTCATTTTGATACTCCACCACCCCGTTTCCCTCTGCCCACCCAAAATGTGAAAATTCATATACATTTTTTACGTTCTCGAGATGTTATATGTTGTATATTCAAAGGATACAGAGCTTATGTTGTCCACATGTAATTTTACTATATATATAAAATGTTGGAACGGAATGTTCCCCTATTAACTTAGGTAAAWATTGATTTGGGGGGAAGGAATGTGAAATCAAATTTATAAAG
TARGET=1350,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01448 Ps_contig_mira-and-tgicl-ass_franssen_14129
SEQUENCE=CCGACCAAATGTAATTTCAGGAATCATCTTAGAAATATGATCCAAGTATTCATTTGCCCCGTGTATAAATTGTGTACCACCTCCTGGAAATTTAAACTTATTTTTGTCTCTGGAAATCCAGTTTTGGCCACCTTTATCTTCAACAAGACGAGTATGAGGAACATTACTGAACCATACCTCATCTCGGCTTTTAGGCCATGGAATTGGAGCACGGTAGCCTTTAGGAGCAGGAACCAAACAATTCAACCTTTTACCTTCCTCTGGACAATGCCGTTCAAATCTCTCTCCTTTCTCCGTGGACGGCAGCTTCTGAATCGCTTCCACATTATCCAAACAAGGTATATACTCACTCATATCACGCGAACACAACCCAAACTTCTTAATCGCGAAACTTTGAACACTCTCAGAACCCTCCTTCTCAGCTTGCGTTTCGTTAACCCAATCATCCACCATACCTGGATCGAATTCCCCAACCTCAAACTCCTCAGACATTGTTCCATTCTCATTCACGATCCCGAATTTCGCCACCGAATCTTCTGTCGGTGGCGGCGGCGGCGGTGGTGGTTGTTTCGTTAGGGCTTTTTCTCGAACAGGTTCTGTGCTGTTGTGATCGATTAGTGAAGAAATATCGAATGATTTGTTGTAATTAGGGGAAATCGAAACCGCTTGATTCGGATCCGGGTCGGAGTCCTGTGTGAAGAAGATGAGTTGTTGGTAGCCATCGGACCAGTGKTTTCCTAAGTAGAAGAAAGTGATGGAAATGAAGAAAAAAAAGGCGATCTTGAGAGCGTGTGTTGATTTGAGAAAATCGAGATTGATTGGAGATTTCATAGTTCAATTTGCGGATCTAACTAGTTCATGAATTCAGCATATCTGAGACGAATGAAGAACTGTGTTTGGATCTTACTGTATTTTGGAGCTAAACTAGTTAGTTACTCAGAGTACTAATTACACTCTGAGCAATCC
TARGET=545,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01449 Ps_contig_mira-and-tgicl-ass_franssen_14144
SEQUENCE=ACAACAATCTTGTTATTTGCTTTCACCGTGGTTGATATTGTCATCGCTGCCACCACCCCTTTTCCGATCACTCTCCCCCTCCCGAACTACCCTCCCCTTCCATCGACTCAAACCAATCAAAGATCGCAGCTTTGCTCTCTCTCTCTCTCTCTCTTTTCACAAAACCTAAGAAATCGCAAGAACCCTCAAAAGAAGAAAACACAAAAACTAGGGTTTGGCAAAAGAATCAATTTGGTATATTTTCTCATTTTCTCTCTCTTTTAAATCAAAATTCCGTGATACTCAAGCAACGGAACAACACAAGATTTCG
TARGET=135,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2464
PRIMER_LEFT_PENALTY=0.117817
PRIMER_RIGHT_PENALTY=0.128591
PRIMER_LEFT_SEQUENCE=TCACCGTGGTTGATATTGTCAT
PRIMER_RIGHT_SEQUENCE=TTCTTCTTTTGAGGGTTCTTGC
PRIMER_LEFT=22,22
PRIMER_RIGHT=196,22
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.871
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=175
PRIMER_PAIR_PENALTY_1=0.2935
PRIMER_LEFT_1_PENALTY=0.117817
PRIMER_RIGHT_1_PENALTY=0.175676
PRIMER_LEFT_1_SEQUENCE=TCACCGTGGTTGATATTGTCAT
PRIMER_RIGHT_1_SEQUENCE=TGTTCCGTTGCTTGAGTATCAC
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=60.118
PRIMER_RIGHT_1_TM=60.176
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.3288
PRIMER_LEFT_2_PENALTY=0.117817
PRIMER_RIGHT_2_PENALTY=0.210973
PRIMER_LEFT_2_SEQUENCE=TCACCGTGGTTGATATTGTCAT
PRIMER_RIGHT_2_SEQUENCE=GTGTTGTTCCGTTGCTTGAGTA
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=60.118
PRIMER_RIGHT_2_TM=60.211
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.3986
PRIMER_LEFT_3_PENALTY=0.269968
PRIMER_RIGHT_3_PENALTY=0.128591
PRIMER_LEFT_3_SEQUENCE=TTTCACCGTGGTTGATATTGTC
PRIMER_RIGHT_3_SEQUENCE=TTCTTCTTTTGAGGGTTCTTGC
PRIMER_LEFT_3=20,22
PRIMER_RIGHT_3=196,22
PRIMER_LEFT_3_TM=59.730
PRIMER_RIGHT_3_TM=59.871
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=0.4048
PRIMER_LEFT_4_PENALTY=0.276218
PRIMER_RIGHT_4_PENALTY=0.128591
PRIMER_LEFT_4_SEQUENCE=GTTATTTGCTTTCACCGTGGTT
PRIMER_RIGHT_4_SEQUENCE=TTCTTCTTTTGAGGGTTCTTGC
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=196,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=59.871
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_01450 Ps_contig_mira-and-tgicl-ass_franssen_14172
SEQUENCE=CTATCTCCATAAGGACAACCTTCCTCGGTATAAAACTTCTTACAATGCCTACCCTTATAAGACCTATTATTCATACTCTCCCCTACAAAAGTACTCGACCCAACCGTCGGAATCTGAAACTCCTCCCTCGGCTGCTCAAGCACGTTCCTCTCTTCCTCATGTGCCGCCACAATTTCCTGCCAATTCGCCGGCGGCCGACGGAGCTCTTCAACACTGTGAGCGAAATTACAGTTCGTTATGTAAGGACAAGTCCCCACACGAAACTTGCAGCAGAGCTTCGTCTTGAAAAACATCTTCCCAATTGCTTTCGCTCGATTGGATCCGCCGCCGCCGCCATTTCCTCCGTCTTGCGAACTGCTTCTCGATTTCTTATTCGGCGGCTCGCTTCCCGAACGCTGTTGTCTCTCGCAATTGGAGTTCGAAGACGGAGTGGTTTCGCCGGCTTCGTTTCCGTTCCAGTATCGGTACTCATCTTCCGTCGCCCAATCTGCTTGGTCACTCGACCAGCTATCAGTGCCGCCTCCGCCTCCGCCGGTGATGATTTGAACGACGTTGCCGTCTCGGTTGTAATCCATTATTACAAAACCCTAACTGTGAGTGTTGGAAAAAATAACTAAAACTACATAGGAGTSYCCGAACAAGAACACGAACACGTGTTAAGTAAGATTAGGAAAATAGAAATT
TARGET=322,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01451 Ps_contig_mira-and-tgicl-ass_franssen_14188
SEQUENCE=TTATGCAAGGCACTCTCCGAAGCCCCTGGGTTCAGTAAGTAGCAATGCAGTCTTTTCCACTGGAAAGTTTAAGAGGAGAAACTTGGAAGCCATTCCGGCTGTTTCTAAGACACGATCAAATTCCATGGCTACAAGTGCTGATTCTCCCTCCCTCCAGCAGAAGCATGGCAAGCATTCTCATGGGAACACTGTTGATGATTCCTTTAGCAAGGAACCTGCGTATCCAGAAGAGTGGAGATTCAACTACCAGGG
TARGET=144,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1901
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.186529
PRIMER_LEFT_SEQUENCE=TCAAATTCCATGGCTACAAGTG
PRIMER_RIGHT_SEQUENCE=ATACGCAGGTTCCTTGCTAAAG
PRIMER_LEFT=115,22
PRIMER_RIGHT=222,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.813
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=108
PRIMER_PAIR_PENALTY_1=0.2142
PRIMER_LEFT_1_PENALTY=0.027715
PRIMER_RIGHT_1_PENALTY=0.186529
PRIMER_LEFT_1_SEQUENCE=AAATTCCATGGCTACAAGTGCT
PRIMER_RIGHT_1_SEQUENCE=ATACGCAGGTTCCTTGCTAAAG
PRIMER_LEFT_1=117,22
PRIMER_RIGHT_1=222,22
PRIMER_LEFT_1_TM=60.028
PRIMER_RIGHT_1_TM=59.813
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=0.2247
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.221115
PRIMER_LEFT_2_SEQUENCE=TCAAATTCCATGGCTACAAGTG
PRIMER_RIGHT_2_SEQUENCE=ACTCTTCTGGATACGCAGGTTC
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=232,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.779
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=0.2488
PRIMER_LEFT_3_PENALTY=0.027715
PRIMER_RIGHT_3_PENALTY=0.221115
PRIMER_LEFT_3_SEQUENCE=AAATTCCATGGCTACAAGTGCT
PRIMER_RIGHT_3_SEQUENCE=ACTCTTCTGGATACGCAGGTTC
PRIMER_LEFT_3=117,22
PRIMER_RIGHT_3=232,22
PRIMER_LEFT_3_TM=60.028
PRIMER_RIGHT_3_TM=59.779
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=116
PRIMER_PAIR_PENALTY_4=0.2629
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.259289
PRIMER_LEFT_4_SEQUENCE=TCAAATTCCATGGCTACAAGTG
PRIMER_RIGHT_4_SEQUENCE=ATCTCCACTCTTCTGGATACGC
PRIMER_LEFT_4=115,22
PRIMER_RIGHT_4=238,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=59.741
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_01452 Ps_contig_mira-and-tgicl-ass_franssen_14193
SEQUENCE=TCGGGGACCACAAGTTTTCTTCCTTCATATCTCCTCCACACACACACTACCTCTTCAATCAACTACATATATATATTTATGGTTCATTAGTCTATTATTGAAGTATTCAAACAACAACTCCACACACCTTAATTTGGTATACTTTCATAACTTGTTCACACAGCAAACTTTGAAGTATAAGAAATTAATTAATGGCTGCTACTGCTACTGCTACTGCTGGTTCATTTTCAATGAAGGGTTCGAAGTTTCGATCGTTGGGACGATACAGTTCGAAGCAAGTTCGTCAGCAGAGAGCAAGGTTATACATAATATGGAGATGTACTGTTTTACTATTGTGCTGGAATGATTAAAAGTAAGTACAAGAATGATGAATATTTGTAATTGCAATTGTAGGTTCTTCTATTATGGGAACTTTATGTAGTAGTTTTTGTTACTACTTAGTAGGGTAGGTGAAGGAGTATGTAATTAAGGAGCTAGATTAAGCAGGAGTTTCTGATTAAACTGTAAATTATATGATATAGTACTCAACCATGTATAGATATAATGATAATTCAAGTTGAMTTTCTTAACAAAAAATT
TARGET=37,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01453 Ps_contig_mira-and-tgicl-ass_franssen_14193
SEQUENCE=TCGGGGACCACAAGTTTTCTTCCTTCATATCTCCTCCACACACACACTACCTCTTCAATCAACTACATATATATATTTATGGTTCATTAGTCTATTATTGAAGTATTCAAACAACAACTCCACACACCTTAATTTGGTATACTTTCATAACTTGTTCACACAGCAAACTTTGAAGTATAAGAAATTAATTAATGGCTGCTACTGCTACTGCTACTGCTGGTTCATTTTCAATGAAGGGTTCGAAGTTTCGATCGTTGGGACGATACAGTTCGAAGCAAGTTCGTCAGCAGAGAGCAAGGTTATACATAATATGGAGATGTACTGTTTTACTATTGTGCTGGAATGATTAAAAGTAAGTACAAGAATGATGAATATTTGTAATTGCAATTGTAGGTTCTTCTATTATGGGAACTTTATGTAGTAGTTTTTGTTACTACTTAGTAGGGTAGGTGAAGGAGTATGTAATTAAGGAGCTAGATTAAGCAGGAGTTTCTGATTAAACTGTAAATTATATGATATAGTACTCAACCATGTATAGATATAATGATAATTCAAGTTGAMTTTCTTAACAAAAAATT
TARGET=67,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01454 Ps_contig_mira-and-tgicl-ass_franssen_14204
SEQUENCE=CCGAATACAAGAAAACTTAAGACCTTACCTAAACTAGGNGACCCACGATAAGAATAGTGACAATAAGAGAAGAAAGACAGAAGGGCAAATTACCGAGAGATCGCTGCTGCATACGAGGTTCTAAGTGATGAAGACAAGCGCACGAGATACGACCGAGGGGAGGACCTTGAAGAATCAGGAATGGGAGGCGGTGGCGGTGGAGGTTTCAATCCCTTCGGCGGTGGTGGACAGCAATTTCACTTTACTTTTGATGGTGGCTTCCCTGGTGGGGGATTTCCCGGCGGCGGCGGTGGTTATGAATTTCATTTTTGATTCAAAAATAGTCTCAATCCTTAAATTTTTCTTAGGGAAAGCTATAGCCTTGATTTCCTCAAGGGACCACAGCTATAATGTTGACTAATCACTTGCTAACGGTTGTTGAGGTAAATACATAGGCCATCCCCAAGTTGCTTGCAAAACAAGACTTTGTTGTACTTTACAGAAATTTTTGCTTTGTGTAAAAGTGATGGGGATAGGTGTTAGTTAAAGAGGCACATTTTGATGTAGACCCATTTAGTTTTATGTGATGGTTGTTACTATGTAAG
TARGET=279,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1554
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.095496
PRIMER_LEFT_SEQUENCE=GGACCTTGAAGAATCAGGAATG
PRIMER_RIGHT_SEQUENCE=ACCTCAACAACCGTTAGCAAGT
PRIMER_LEFT=161,22
PRIMER_RIGHT=423,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.1554
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=0.095496
PRIMER_LEFT_1_SEQUENCE=GACCTTGAAGAATCAGGAATGG
PRIMER_RIGHT_1_SEQUENCE=ACCTCAACAACCGTTAGCAAGT
PRIMER_LEFT_1=162,22
PRIMER_RIGHT_1=423,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.1630
PRIMER_LEFT_2_PENALTY=0.067516
PRIMER_RIGHT_2_PENALTY=0.095496
PRIMER_LEFT_2_SEQUENCE=CTTGAAGAATCAGGAATGGGAG
PRIMER_RIGHT_2_SEQUENCE=ACCTCAACAACCGTTAGCAAGT
PRIMER_LEFT_2=165,22
PRIMER_RIGHT_2=423,22
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.3749
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.315058
PRIMER_LEFT_3_SEQUENCE=GACCTTGAAGAATCAGGAATGG
PRIMER_RIGHT_3_SEQUENCE=TTTACCTCAACAACCGTTAGCA
PRIMER_LEFT_3=162,22
PRIMER_RIGHT_3=426,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=59.685
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=265
PRIMER_PAIR_PENALTY_4=0.3749
PRIMER_LEFT_4_PENALTY=0.059875
PRIMER_RIGHT_4_PENALTY=0.315058
PRIMER_LEFT_4_SEQUENCE=GGACCTTGAAGAATCAGGAATG
PRIMER_RIGHT_4_SEQUENCE=TTTACCTCAACAACCGTTAGCA
PRIMER_LEFT_4=161,22
PRIMER_RIGHT_4=426,22
PRIMER_LEFT_4_TM=59.940
PRIMER_RIGHT_4_TM=59.685
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_01455 Ps_contig_mira-and-tgicl-ass_franssen_14211
SEQUENCE=CCCCAAACCCGAAACCCAAAAACCCAATCTTCATCTTCYCGAAGCGGCATGAAACTAATCGTGCCTCTTCAAGGTGTAGTTCAAGGTCGCAGTGGCTTTCTCCTCGGTATTTCAATTCCTTGTGCTCTCTTTTACTTCCTTCAACTTTACCTCAAACGACGTCGTCCTAATCCTAACCCTTCTTCTCCTTCTTCTTCTTCTCCCACTCTTCTTCTTACAAGGTCTTCTTCCAGGTCTAATCTATCCTCTCGCGGATCAATTTCGCGTGTTCGTCTTTCTAGACTCGCTACGGTTCTATCTAAGCCTGATGATTCTTCTTTTATTATCGTCGGGTTGGA
TARGET=188,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01456 Ps_contig_mira-and-tgicl-ass_franssen_14226
SEQUENCE=GGAGGGGAGAAACGAAGAAACCAACGATATTTGGGAAGGAGATTCCGCTAATGGTGCAGATCAAAACCAGAGGGATTGGTCGTCTTGTGTTTTCGTCGAGGATTTTGGAATGGGGCACCAAATCCCAATCCCAATCCAATCCAATCCCCACCGTAGTTCCCTTTGCCTCAACCACGGTGGCCACTCATGGATTATCACGCCCACGGACACCACCCTTCTTCTGACTCCCGCCACCCCAACCACCCCMAAGACCCCCACCCTCTT
TARGET=131,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01457 Ps_contig_mira-and-tgicl-ass_franssen_14242
SEQUENCE=TACATCCCGGTTCCGTTTTCCTAGATGCAGCTTCACAAGAAACGTTAAGGAAGGTCATAAATAAYACAAGCGCCGATTCGTATCCTTCAACTCGTCTCAACATGCGTAGATCTGCGATGAAGAATTCACTGGTGAGGATCGGTGGTGTGGAAGGAGAATGGGTGAAAAGAGCTTTGACAGCGTTGATTCGACCTTCTTCGCACCAACAGAGGCGGAGATCGGCGTTTGAGCCAAGACCTAGTCGTCTCTCCGTCATGACCAAGGCTGAAGATCTTTGATTTTGCCTGTTTTCGTTTTTCAGGTTTGATCTTGCTAATCACGCTTTTGCGGTGGTTGATTTACACCCACTGCGTTGATCTGCCGCCAGATCGGTTGATCCGGCGAGCATTTAGGTTAAAGCGTGTCATCTCTGGCAAGTTTTGGGAGCATATTGATGAATCAGACATCGGAGAAAATCGTTGAAGAATTTCTGATTAGATCTGTTGAGATTCAATTTGGACTCATCATCATCATCAGTATTGCATTTTGTACATAACATTTGCTATGGAAGCTGAGAATATTGGTACTTTTTTGATTTTATTTCCAGTACTAAA
TARGET=501,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01458 Ps_contig_mira-and-tgicl-ass_franssen_14256
SEQUENCE=TGGGAGTGATGTTTCTGTTTTTTTGTTTGTGGTGGTGGTGGGTGGTGGTGGTGGTGGAGGAGATGTTTTTGTTTTTGTAGTGGTGGTTTTTAGTGTCTGGGTTTGGGTTGTTTGGGGTTTTTCTTTGGTTTTGATGGATGAGATGTTAAACGAAGGGATTSAAGTTGGGGGTTGTTTACA
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01459 Ps_contig_mira-and-tgicl-ass_franssen_14256
SEQUENCE=TGGGAGTGATGTTTCTGTTTTTTTGTTTGTGGTGGTGGTGGGTGGTGGTGGTGGTGGAGGAGATGTTTTTGTTTTTGTAGTGGTGGTTTTTAGTGTCTGGGTTTGGGTTGTTTGGGGTTTTTCTTTGGTTTTGATGGATGAGATGTTAAACGAAGGGATTSAAGTTGGGGGTTGTTTACA
TARGET=41,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01460 Ps_contig_mira-and-tgicl-ass_franssen_14273
SEQUENCE=GGTTCTGTGRGTGACTCAATCAATCAATACTGTACTAGTACTTATTATACTATCACAGACTCTTCTTGCGTTTTGGCTTCTTTCATTTCTTCACTCTTAAATATAATAATAACAATAATCTCTTTTCATGAAGATGGAGATGAAGAAAGGAGTTGAAACTGTGGTGATTAATAATGGAAACTTTGTGGAAGAAGAAGAACTCTCGAGCTGTAAATTCTCGCATCTTCTTGCTTCCAAGGATCGTGATTTTCTTCTATCTTCAACTGGAGCTCAGGTTAAAGTTTCAGAACTTGAAGGAAAAGTAGTGGGATTATTATTCGCTGCCAACTGGTACCCACCATGTCGTGGATTCACCCAGTTGCTCATTGGAAATATACCAACAACTAGAAAAACAAAAATAAATACCCTTCCTTTGAAATAGTTTATGTATCTTCTG
TARGET=16,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01461 Ps_contig_mira-and-tgicl-ass_franssen_14273
SEQUENCE=GGTTCTGTGRGTGACTCAATCAATCAATACTGTACTAGTACTTATTATACTATCACAGACTCTTCTTGCGTTTTGGCTTCTTTCATTTCTTCACTCTTAAATATAATAATAACAATAATCTCTTTTCATGAAGATGGAGATGAAGAAAGGAGTTGAAACTGTGGTGATTAATAATGGAAACTTTGTGGAAGAAGAAGAACTCTCGAGCTGTAAATTCTCGCATCTTCTTGCTTCCAAGGATCGTGATTTTCTTCTATCTTCAACTGGAGCTCAGGTTAAAGTTTCAGAACTTGAAGGAAAAGTAGTGGGATTATTATTCGCTGCCAACTGGTACCCACCATGTCGTGGATTCACCCAGTTGCTCATTGGAAATATACCAACAACTAGAAAAACAAAAATAAATACCCTTCCTTTGAAATAGTTTATGTATCTTCTG
TARGET=188,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01462 Ps_contig_mira-and-tgicl-ass_franssen_14277
SEQUENCE=TGTGGATTGAAGGATGAAGAGAATTGGATTGAAGTGGTGGTTAACTGTTTYGTGAGTTTGTTACAGTTGTTGAAGGAATTGAAGAGAAGAAGAAGAATCTGGATGAAGAGAAGTGAATTGATTTTATAGAGTTATGAGAGGGATTGGAAGTTTGTYAGAAAAAATTCTGTGTGAGAAATTCTGTTATTCGATTGTGAGTTCATTTTTTANGCAGAAATTTAATTTGATTCTTATNNAATATNTNTTGTAGA
TARGET=85,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01463 Ps_contig_mira-and-tgicl-ass_franssen_14288
SEQUENCE=GTTCAAATGGGGGAGTAAAGCGTGGAAAAGGAGGCAAAAAGAGAGACATACAGTTCTACGAATCATTCACCTTGAATGGTGTTGATTACTCACTCTTTGATACTGTTTATCTTAAAAGTGGAGCGCAACCTGAACCACATATTGGAAAAATCATCAAGATATGGGGAACTCCTAGTGACAAGACTAGGAAAATTAAGATTCAGCGGTTCATTCGTCCGTGTGAGATCTCAAAGCTTTTGACCGGGATTAAGATTTATTATAATGAATTATTCTTGTCTTGTGGTGATTGCACTGGACTTGCCCTTATCAATCCTCTGGAATCTATTACTGGGAAATGCAATGTTGTTTGCATTTCTAAGGATTCTAGGAATCCACAGCCATCAGATGAAGCATTTCGGAAAGCTGGCTTTGTGTTCTATCGATACTTTGATGTTGGGCAGTGTAAAATAGTGGAGGAAGTAGATGACAAGATTGCTGGAGTGGAAGTTAAAAACTTTTTTTAACAAAACGGCATAGTCAAAAGCCTGCTGCTGCTGCTGCTAGTAAAACTATTTGTTTATAACGAAGAAAAGGAAGGTTAGGTAGGGGAATGACTAGAAAGTAG
TARGET=525,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2962
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.263882
PRIMER_LEFT_SEQUENCE=CTTTGATGTTGGGCAGTGTAAA
PRIMER_RIGHT_SEQUENCE=CATTCCCCTACCTAACCTTCCT
PRIMER_LEFT=424,22
PRIMER_RIGHT=591,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=59.736
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.3716
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.263882
PRIMER_LEFT_1_SEQUENCE=GGGCAGTGTAAAATAGTGGAGG
PRIMER_RIGHT_1_SEQUENCE=CATTCCCCTACCTAACCTTCCT
PRIMER_LEFT_1=434,22
PRIMER_RIGHT_1=591,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=59.736
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=0.4384
PRIMER_LEFT_2_PENALTY=0.174518
PRIMER_RIGHT_2_PENALTY=0.263882
PRIMER_LEFT_2_SEQUENCE=ACAAGATTGCTGGAGTGGAAGT
PRIMER_RIGHT_2_SEQUENCE=CATTCCCCTACCTAACCTTCCT
PRIMER_LEFT_2=465,22
PRIMER_RIGHT_2=591,22
PRIMER_LEFT_2_TM=60.175
PRIMER_RIGHT_2_TM=59.736
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=127
PRIMER_PAIR_PENALTY_3=0.5652
PRIMER_LEFT_3_PENALTY=0.032347
PRIMER_RIGHT_3_PENALTY=0.532832
PRIMER_LEFT_3_SEQUENCE=CTTTGATGTTGGGCAGTGTAAA
PRIMER_RIGHT_3_SEQUENCE=TCATTCCCCTACCTAACCTTCC
PRIMER_LEFT_3=424,22
PRIMER_RIGHT_3=592,22
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=60.533
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=0.6332
PRIMER_LEFT_4_PENALTY=0.032347
PRIMER_RIGHT_4_PENALTY=0.600830
PRIMER_LEFT_4_SEQUENCE=CTTTGATGTTGGGCAGTGTAAA
PRIMER_RIGHT_4_SEQUENCE=CCCCTACCTAACCTTCCTTTTC
PRIMER_LEFT_4=424,22
PRIMER_RIGHT_4=587,22
PRIMER_LEFT_4_TM=60.032
PRIMER_RIGHT_4_TM=59.399
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=164
=
PRIMER_SEQUENCE_ID=SSR_01464 Ps_contig_mira-and-tgicl-ass_franssen_14295
SEQUENCE=TTTTTGTTTAATTTTAGTTAAGTTACTATAAGTTACGTATTAATTTTAATTTTTAGGGTTAAGTTTTGGTTAAAGGTACCCAAACGGTACCGTTAAAACTAAGTTTACGTATTCTAAAAGAAAGTAGGAAAAGTTACTATCTTTCTAATAATGTGAAAGTGAAACTCAAAAGACAAGAAATCAATTCCAAGAATATAGAATCATTGACACAAGAGAAAGAAGTTCCGCGCGTCGTCATCGATGTTGAAGCTAAAGAAGAGGAGGAAGAAGAAGAAGTTGAGATTGAAATTGAAAACAATGATGATGTTGAAATTGAAAACAATGATGATGNGTTGAGAAAAAGAGTGGAAGAGTTTATTGAGAAAGTTAACAAAAATGGAAAGAGGAATTGTTGAGCACATCAACATTAGTATATAGGAAAAAGTAATAATGGGTATTTTGGCTATATGAAAATGTTGTTAGTNTTTTGTTGTATTATGAAGTATTAGTTTTGTCATAAGGTNCTAA
TARGET=264,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1883
PRIMER_LEFT_PENALTY=0.071684
PRIMER_RIGHT_PENALTY=0.116582
PRIMER_LEFT_SEQUENCE=ACACAAGAGAAAGAAGTTCCGC
PRIMER_RIGHT_SEQUENCE=GTGCTCAACAATTCCTCTTTCC
PRIMER_LEFT=206,22
PRIMER_RIGHT=398,22
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=60.117
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=193
PRIMER_PAIR_PENALTY_1=0.1895
PRIMER_LEFT_1_PENALTY=0.071684
PRIMER_RIGHT_1_PENALTY=0.117817
PRIMER_LEFT_1_SEQUENCE=ACACAAGAGAAAGAAGTTCCGC
PRIMER_RIGHT_1_SEQUENCE=TTGATGTGCTCAACAATTCCTC
PRIMER_LEFT_1=206,22
PRIMER_RIGHT_1=403,22
PRIMER_LEFT_1_TM=59.928
PRIMER_RIGHT_1_TM=60.118
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.3809
PRIMER_LEFT_2_PENALTY=0.264300
PRIMER_RIGHT_2_PENALTY=0.116582
PRIMER_LEFT_2_SEQUENCE=CGTCATCGATGTTGAAGCTAAA
PRIMER_RIGHT_2_SEQUENCE=GTGCTCAACAATTCCTCTTTCC
PRIMER_LEFT_2=232,22
PRIMER_RIGHT_2=398,22
PRIMER_LEFT_2_TM=60.264
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.3821
PRIMER_LEFT_3_PENALTY=0.264300
PRIMER_RIGHT_3_PENALTY=0.117817
PRIMER_LEFT_3_SEQUENCE=CGTCATCGATGTTGAAGCTAAA
PRIMER_RIGHT_3_SEQUENCE=TTGATGTGCTCAACAATTCCTC
PRIMER_LEFT_3=232,22
PRIMER_RIGHT_3=403,22
PRIMER_LEFT_3_TM=60.264
PRIMER_RIGHT_3_TM=60.118
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.3949
PRIMER_LEFT_4_PENALTY=0.278329
PRIMER_RIGHT_4_PENALTY=0.116582
PRIMER_LEFT_4_SEQUENCE=GTCGTCATCGATGTTGAAGCTA
PRIMER_RIGHT_4_SEQUENCE=GTGCTCAACAATTCCTCTTTCC
PRIMER_LEFT_4=230,22
PRIMER_RIGHT_4=398,22
PRIMER_LEFT_4_TM=60.278
PRIMER_RIGHT_4_TM=60.117
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=169
=
PRIMER_SEQUENCE_ID=SSR_01465 Ps_contig_mira-and-tgicl-ass_franssen_14326
SEQUENCE=CTTTTTCTATTCGCCGTTTCTCTTACTATCACCACTCTCTCTCTCTCTCCCTGAATTTTGGACACAGATCTTCATCTCCACAAAATGCGCAATTTCAATTTCTTCCACCGTTTCTCCAAAACCTTCCGTGACTACGATTCTCAGTTCAAGCTTGTCCTCCTCTGCACCACCGTAAGTGGCGGTGGCCTTTTGGCATACGGTGAGGCTGTTGCTACATCTGAAGCGGCGGTACCTGAGAAGAAAAAGGTGTTGGTGCTGGGAACTGGTTGGGCAGGAACGAGTTTCTTGAGGAATCTTAATGATCCTCGATATGAGGTTCTACGTTGWCTCTCCTCGGAATTATTTTGCATTCACTCCTTTGCTGCCCAGTGTTACCTGTGGCACCATCGAGGCTCGCAGCATTGTTGAACCCGTCCGCAATATCTTCAGGAAGAAACATGTGGACTCTCGATTCAGTGAAGCAGAATGTATCAAGATTGATGCAGTAAACAGAAAAGTTTACTGTCGAGCTAATATTAACAATAATTCGAATAGAAAAAAGATGAATTCGTTGTGGACTATGACTAC
TARGET=35,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01466 Ps_contig_mira-and-tgicl-ass_franssen_14365
SEQUENCE=TTACCCTATCCTCTCAACCAAAGCCAAACAACTCTCCCACTGCCAACCCGTAAATACCTCACCCTGTTCCATCAATCCTCAGTGGCCCTCCAGATAAACTCCCTCTTCCAATNCCGGGAGCAAATCACAATTATCGCAGCAGCAACAGAAACAACAGCAGCAACAACACCAACACCAGCAGCAGCAAATAGCAAAGCAGAACTTGCAACAGGCCCAACTGTTCTTTACAAATCCTTACATGCACTCGCAAGTTGCACAATCCAACAGTCCCACCTCTACTACCTCAGCTGCAACTGGGTATTATCTTCAACGGCGCGGCCCCGAGCAAGTGCAACGACAGGGATCTGGTGGCACATCCTCAAATGGCGCAGCTGCAAACAATTCGAAAGGCAGTACCTTGAACACACAGGGTCTTCTGCATCCTTCCCAGTTTGCGGCAATGCCACCTTCTGGGAACCACCATCAATTTGTTCCAGCTGGTTTCTATAATGTTCAACCGGTGCCCACAGCAGTTCAGGTGAAGCCAGCAGAGCAGAAACAACCAGCAGGTGAGTAGAATGATATCTTCCCTTAGTTGCACCCCATGTGCATATGGTGAGGAAAGAAGAAAAGTAACTGAATGAGATATGAATTTTGAAGTTGGTAAGAAAAGATGGTATCTTCTGAAGTGGTGGGGCCAAGATGTTGATGTCGTATTGTGCCGCGTGCCTTGAGAAGAAGATACGGAAAAGCAAGCAAGCTCGAGCTGATCACGGGCAACACAAGATATGATTTGGAGTCCACAATTGTCCACAATTTTGGATGGGGGAAATCGACCAGAACTTTGATGTGCATGAGTTGTGTTTAGTAATTCATTTGTACACTTGTGTTTATAATCTGACAAGGTTGATCTAATTAAGTCTAATACTAAAATTCGTATTTTTAAAACGG
TARGET=729,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7029
PRIMER_LEFT_PENALTY=0.143555
PRIMER_RIGHT_PENALTY=0.559375
PRIMER_LEFT_SEQUENCE=GCCTTGAGAAGAAGATACGGAA
PRIMER_RIGHT_SEQUENCE=CATGCACATCAAAGTTCTGGTC
PRIMER_LEFT=706,22
PRIMER_RIGHT=834,22
PRIMER_LEFT_TM=59.856
PRIMER_RIGHT_TM=60.559
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=129
PRIMER_PAIR_PENALTY_1=0.7029
PRIMER_LEFT_1_PENALTY=0.143555
PRIMER_RIGHT_1_PENALTY=0.559375
PRIMER_LEFT_1_SEQUENCE=GCCTTGAGAAGAAGATACGGAA
PRIMER_RIGHT_1_SEQUENCE=TCATGCACATCAAAGTTCTGGT
PRIMER_LEFT_1=706,22
PRIMER_RIGHT_1=835,22
PRIMER_LEFT_1_TM=59.856
PRIMER_RIGHT_1_TM=60.559
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=0.7387
PRIMER_LEFT_2_PENALTY=0.203324
PRIMER_RIGHT_2_PENALTY=0.535412
PRIMER_LEFT_2_SEQUENCE=CAACCAGCAGGTGAGTAGAATG
PRIMER_RIGHT_2_SEQUENCE=ATCAAAGTTCTGGTCGATTTCC
PRIMER_LEFT_2=538,22
PRIMER_RIGHT_2=827,22
PRIMER_LEFT_2_TM=59.797
PRIMER_RIGHT_2_TM=59.465
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.7974
PRIMER_LEFT_3_PENALTY=0.261998
PRIMER_RIGHT_3_PENALTY=0.535412
PRIMER_LEFT_3_SEQUENCE=GTGCCTTGAGAAGAAGATACGG
PRIMER_RIGHT_3_SEQUENCE=ATCAAAGTTCTGGTCGATTTCC
PRIMER_LEFT_3=704,22
PRIMER_RIGHT_3=827,22
PRIMER_LEFT_3_TM=60.262
PRIMER_RIGHT_3_TM=59.465
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=0.8002
PRIMER_LEFT_4_PENALTY=0.264820
PRIMER_RIGHT_4_PENALTY=0.535412
PRIMER_LEFT_4_SEQUENCE=GATGTTGATGTCGTATTGTGCC
PRIMER_RIGHT_4_SEQUENCE=ATCAAAGTTCTGGTCGATTTCC
PRIMER_LEFT_4=680,22
PRIMER_RIGHT_4=827,22
PRIMER_LEFT_4_TM=60.265
PRIMER_RIGHT_4_TM=59.465
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_01467 Ps_contig_mira-and-tgicl-ass_franssen_14386
SEQUENCE=TCGGGGGAATCGAAGTGTAGTCCAAGTATCGAACGAACAAGTTCCGCCGTTAAATTAAAATCAAAATAATTTCCGCCTTTGCTTCACGGCCATTGCCTCTGCTCTGCTCTGCCTGATGCTTCGCCTTCCATCATCACGTTGAGGCTCTTTCCCTCCTTTGAATTCTGCAGCTCTGAGTAAAGATCATCGGGCTGTAGAGAATGGCAGAAACTATGTCCAGCTTCTGGGGTCCAGTTACATCCACCAAAGAGTGCTGTGAAGAAAATTTTATACATTCGTGTTACATTGCAGAACTTTATAACACTATATCCAACATTCCGACAATTATTTTGGCTCTGATTGGTCTTATAAATGCTCTAAGACAGCGATTTGAGAAAAGGTTCAGTGTCCTTCATTTATCAAACATGACACTTGCCATTGGGAGCATGTTGTACCATGCCACACTGCAACGTGTGCAACAACAAGGGGATGAAACTCCTATGGTATGGGAGGTGCTTCTGTACATGTACATCCTCTACTCTCCAGATTGGCACTATCGCAGTACAATGCCTATCTTCCTATTTCTGTATGGTGCTGGTTTTGCAATCGCCCATTCCGTGTTCCGTTTTGGTGTCGGCTTCAAAGTGCATTATATAGTTCTGTGTCTGCTGTGTACTCCAAGAATGTACAAGTATTACATTCACACTCAAGATGTGTTGGCCAAGCGGCTAGCTAAGCTATTTTTAGGCACTCTTATATTAGGAAGTTTGTTTGGGTTTTGTGACCGTGTTTTCTGCAACGAGATATTGGGCTGGCGTATTAACCCTCAGGGTCATGCTTTGTGGCATGTCTTCATGGGTTTCAATTCCTACTTTGCAAATACATTCTTGATGTTTTGCCGTGCTCAACAGCGTGAGTGGTCTCCGAAAGTTGTTTATTTAATGGGTGTTTTACCATATGTGAAGATTGAGAAACCAAAAAGCCAGTGACAGATTTGGAAGCTTTTTTCTCATGATACATTATAGCTATAGACAAGTTGTTGACAAAATTATTAAATGGGTGTCTTTCTTTTCTTTAGTTCTTTTTCACTTTGTGGTTTTGATTGGCTTAGAAATGATATTGAATATTTGCATATTTTATTGTAGAAGTAGGATGTGTACCATATTTATCTTGGACCTTAACTCAATATTGGCAAAAGTGAACGTTGTTTTGAAMAAAAAAG
TARGET=97,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01468 Ps_contig_mira-and-tgicl-ass_franssen_14393
SEQUENCE=CCCTCTCTCATATGCCACTGACATAGCAAAAAAAATAACTCATTTTATTTTTTTCTTCTTCTTTCTCCATACAAACAACTCCACAAACCGCCTTCTTTCTCTTCATTTTTCCCCATCATTATCCATACAAAAAATATCTCATCGCTCACATATCACTTCCTCTCATCTCTCAACAATCACTTCCACTTTTAAAAAAATAAATAAAATAAGTTTTATCATTTTCTTYKKSKACACACCAAAACCGCCTCAACCTCATCTCACATAACATCATACTTCTAGTTTCTTAATCATCCTTACCATTTCATTTCACTCTCTTCAAACATCAATCTTTAAGTTTCTTAATCATCATCATCAACCAATATCCTACTCATCACAACTCACTTAACTCTTTCATCAAACTACAACATAAACCNTCATCATCTTCACAAACACCATATAAAGTCAAA
TARGET=342,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01469 Ps_contig_mira-and-tgicl-ass_franssen_14394
SEQUENCE=TTTCATTTCACTCTCTTCAAACATCAATCTTTAAGTTTCTTAATCATCATCATCAACCAATATCCWACTCATCACAACTCACTTAACTCTTTCATCAAACTACAACATAAACCATCATCATCTTCTCAAACAACCATATAAAGTCAAACACGAACACCGTCGGGTATTTTTTTAAGAGAGTACGTGATGATTTATTTGTTTTATTTTTATTATCTTTTGATGTTTCTCTGTTGTCTTAATATGAGTTAGAGAAAGATTAAGTTGTGTTTGTTGTTGTTTCTCTTTGCAAAGCCATGAGAGAGGTAGAATCATAGAGTTGTTTTAATTTTTTAAAATTGTTTTT
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01470 Ps_contig_mira-and-tgicl-ass_franssen_14397
SEQUENCE=AAACTCTACAAGACACTAACCCTACGTCTTCAACTTCGCCACAATCATCACTGTTCCTTTCAACAAATCCAGGTGCTACAAATCTTGTTCCTTCTACCACACCGCCAACGAATCCAGCAAACTCGCCAGTTCCTGTTACAAATCCTGTTACAGCTCCTGTAACAGTTCCTGTGAGTTCTTATCCACCACCTTCACCGTTATCAGGAGCCGTTCCTGTTCCTGTTACAAATCCTCAGCTTCAGCCTCCTCCTTCAAACATGAATCCTCCTCCATCTTCATCTTCATCTTCTCAAGGAGGAGGTGGTGGTAGCTGGTGTGTGGCTAAACCTGGAGTTTCACAAACTACACTTCAGACTGCTTTGGATTATGCTTGTGGGACTAATGGTGCTGATTGTTCACAAATACAACAAGGTGGAATCTGTTATAATCCTAATTCACTTTCAAACCATGCTTCTTTTGCTTTCAATAGCTACTACGTAAAGAATCCTGCTCCAACTAGCTGTGACTTTGGAGGGGTTGCTACCATTGTTACCACCAATCCTAGTACTGGTACTTGCATCTTTCCATCATCTTCAGGAGGAGCAGGCTCATCCGGCTCTGGTTCTAACTCACCTTCAATAGGTTTTGGTGGGCCTTCAACCTCTCCGGATTCATCTCACTCAACTGGTGTGAGGCCTTTGCTAAGTTTCATGATTCTGATGACATTATTGGCCAGCGGAAGACTCGCGAGTCCATAGATTAACTTCAATACTCTAGCTCCATTTTGGAGTTTGTATACAATTCTACATGCAGGTGTAGAAAAGTAGAAAGATAGTAGAGTAGAAGAGAGAGTTTGTGACGATGATGATGATGATGATGATGATAGTTGATAGAAAACTAAAGTTCCTCAATCTTGTTTTAACTTTAGCTTAAGAAG
TARGET=840,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01471 Ps_contig_mira-and-tgicl-ass_franssen_14402
SEQUENCE=TGAAACTGTTGGTACTGTTATTGTCGTTGTTGTTGTTGAAGTTCTGAAGAAAACGAAACTCGTTTTCGTTAGCGTTTTCTTTCTCCGCTACGTCGTCGTTTTGTTCTTGTTTAGAAACAAGAGTGGGTTTCCATGGAGGAAGTTGCGCGAGTCGATCGATGGCGGGCTTGGCTTTATTAATGAGCCAGTCAAGGGCTTTACTGGGCCGGTCAAAGCCCAACCGATCTTGAACATCGTAGAATTCAATAGCGGTGTGGGCCGATAAACGGACCCGCCGGTCTCTCGGGCCTTTCGCGGTGCAAACTTTACTGTGACGGTCTTTTCTTCCTCTTGACCGTATAATGTGACCGCCGTCAACTTCCACTATCTCACCGCCATTCATTATTCCTACATAATCAACTGAACGACACCGTTTCTTTCCCCGTGGTAACGGAACGGTCCCTTCTGTTAATTCTAATGGTTCTACATGTTTTGAGTTTAATACGGCGTCGTTTTGGTGAAGGAGTTGATGGTGGGTGTTGCTTAGAGGATCCAGACTCGGACAGGTTATTGTTTTTGTTTTGGTTGTTTTATTTCTTGTTCTAGTGATGGTGGTGGTGTTGATTAATTCTTTTCTTTGTTGTGAAGTAAAGTTTYGCCTATTACWAATTTATTTTTCGTTTTTTTCCATGACGTAAATTAGGTTAAAATTTTGAAGTACTAATTTAGGTCGAGGGGAAGAGAGAAGAGGA
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01472 Ps_contig_mira-and-tgicl-ass_franssen_14403
SEQUENCE=TACGTCGGGGTCGTCAACTTCCTCCATGGAAACCCACTCTTGTTTCTAAACAAGAACAAAACGACGACGTAGCGGAGAAAGAAAACGCTAACGAAAACGAGTTTCGTTTTCTTCAGAACTTCAACAACAACAACAACGACAATAACAGTACCAACAGTTTCATTCCGTTTGAAACGGAAACGACGTCGTCTTCGCCGATTCAGTTTCAGACTTATAATAGTACCACTCCTGATTTGCTTTCCAGAACGAATAGTAATGATTTGCGTCTCTCGCTTCAACAGAATCGGAATCAGCAGGCGCTATTCGCCGGGAACTTTGATGGAATTCCGGTAGCATGGAATTCCGGTGGTGGTGGTGGTGGTGGTGGTGGTGCTACTACTATTGATTACG
TARGET=122,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2847
PRIMER_LEFT_PENALTY=0.043121
PRIMER_RIGHT_PENALTY=0.241544
PRIMER_LEFT_SEQUENCE=GTAGCGGAGAAAGAAAACGCTA
PRIMER_RIGHT_SEQUENCE=GTTCTGGAAAGCAAATCAGGAG
PRIMER_LEFT=68,22
PRIMER_RIGHT=246,22
PRIMER_LEFT_TM=60.043
PRIMER_RIGHT_TM=60.242
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.2847
PRIMER_LEFT_1_PENALTY=0.043121
PRIMER_RIGHT_1_PENALTY=0.241544
PRIMER_LEFT_1_SEQUENCE=GTAGCGGAGAAAGAAAACGCTA
PRIMER_RIGHT_1_SEQUENCE=TTCTGGAAAGCAAATCAGGAGT
PRIMER_LEFT_1=68,22
PRIMER_RIGHT_1=245,22
PRIMER_LEFT_1_TM=60.043
PRIMER_RIGHT_1_TM=60.242
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.2891
PRIMER_LEFT_2_PENALTY=0.047586
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=AGAAAACGCTAACGAAAACGAG
PRIMER_RIGHT_2_SEQUENCE=GTTCTGGAAAGCAAATCAGGAG
PRIMER_LEFT_2=79,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=59.952
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.2891
PRIMER_LEFT_3_PENALTY=0.047586
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=AGAAAACGCTAACGAAAACGAG
PRIMER_RIGHT_3_SEQUENCE=TTCTGGAAAGCAAATCAGGAGT
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=245,22
PRIMER_LEFT_3_TM=59.952
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=167
PRIMER_PAIR_PENALTY_4=0.3430
PRIMER_LEFT_4_PENALTY=0.101479
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=GGAGAAAGAAAACGCTAACGAA
PRIMER_RIGHT_4_SEQUENCE=GTTCTGGAAAGCAAATCAGGAG
PRIMER_LEFT_4=73,22
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=59.899
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=174
=
PRIMER_SEQUENCE_ID=SSR_01473 Ps_contig_mira-and-tgicl-ass_franssen_14403
SEQUENCE=TACGTCGGGGTCGTCAACTTCCTCCATGGAAACCCACTCTTGTTTCTAAACAAGAACAAAACGACGACGTAGCGGAGAAAGAAAACGCTAACGAAAACGAGTTTCGTTTTCTTCAGAACTTCAACAACAACAACAACGACAATAACAGTACCAACAGTTTCATTCCGTTTGAAACGGAAACGACGTCGTCTTCGCCGATTCAGTTTCAGACTTATAATAGTACCACTCCTGATTTGCTTTCCAGAACGAATAGTAATGATTTGCGTCTCTCGCTTCAACAGAATCGGAATCAGCAGGCGCTATTCGCCGGGAACTTTGATGGAATTCCGGTAGCATGGAATTCCGGTGGTGGTGGTGGTGGTGGTGGTGGTGCTACTACTATTGATTACG
TARGET=345,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01474 Ps_contig_mira-and-tgicl-ass_franssen_14407
SEQUENCE=CTATATTTCAATAAATCAATTAACCATTACAAAAATTTAAAAAATTATAAGGAACCACAGTTGATAATGTGAAGGATTTCTCAACTTAATAAAGTAATTTCACTAATTTAAAAGATTCTCTTCTCTGCCAACTATGTCCTTTCTTGGAGATTCAACATAACAAGCTTTGCAGAGGCATGAGAGAAACTTATCAACACTTTTCCCCCCACCGAAGACACAAACTATGAACATCACCATCATCATCATCAAGTGTAAGTAATCAAATCAGACGGTTGACCAAGCAAAG
TARGET=235,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5310
PRIMER_LEFT_PENALTY=0.482784
PRIMER_RIGHT_PENALTY=1.048185
PRIMER_LEFT_SEQUENCE=GCCAACTATGTCCTTTCTTGGA
PRIMER_RIGHT_SEQUENCE=GGTCAACCGTCTGATTTGATTA
PRIMER_LEFT=126,22
PRIMER_RIGHT=277,22
PRIMER_LEFT_TM=60.483
PRIMER_RIGHT_TM=58.952
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=1.7226
PRIMER_LEFT_1_PENALTY=0.482784
PRIMER_RIGHT_1_PENALTY=1.239865
PRIMER_LEFT_1_SEQUENCE=GCCAACTATGTCCTTTCTTGGA
PRIMER_RIGHT_1_SEQUENCE=GGTCAACCGTCTGATTTGATTAC
PRIMER_LEFT_1=126,22
PRIMER_RIGHT_1=277,23
PRIMER_LEFT_1_TM=60.483
PRIMER_RIGHT_1_TM=59.760
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=1.8512
PRIMER_LEFT_2_PENALTY=0.482784
PRIMER_RIGHT_2_PENALTY=1.368450
PRIMER_LEFT_2_SEQUENCE=GCCAACTATGTCCTTTCTTGGA
PRIMER_RIGHT_2_SEQUENCE=TGGTCAACCGTCTGATTTGAT
PRIMER_LEFT_2=126,22
PRIMER_RIGHT_2=278,21
PRIMER_LEFT_2_TM=60.483
PRIMER_RIGHT_2_TM=60.368
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=153
PRIMER_PAIR_PENALTY_3=2.0177
PRIMER_LEFT_3_PENALTY=0.482784
PRIMER_RIGHT_3_PENALTY=1.534890
PRIMER_LEFT_3_SEQUENCE=GCCAACTATGTCCTTTCTTGGA
PRIMER_RIGHT_3_SEQUENCE=CTTGGTCAACCGTCTGATTTG
PRIMER_LEFT_3=126,22
PRIMER_RIGHT_3=280,21
PRIMER_LEFT_3_TM=60.483
PRIMER_RIGHT_3_TM=60.535
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=2.0801
PRIMER_LEFT_4_PENALTY=1.031944
PRIMER_RIGHT_4_PENALTY=1.048185
PRIMER_LEFT_4_SEQUENCE=ATAACAAGCTTTGCAGAGGCA
PRIMER_RIGHT_4_SEQUENCE=GGTCAACCGTCTGATTTGATTA
PRIMER_LEFT_4=156,21
PRIMER_RIGHT_4=277,22
PRIMER_LEFT_4_TM=60.032
PRIMER_RIGHT_4_TM=58.952
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=122
=
PRIMER_SEQUENCE_ID=SSR_01475 Ps_contig_mira-and-tgicl-ass_franssen_14408
SEQUENCE=GGTTTGGGTAAAGGAAGTACTTTCTTCCTTTTTGTATTGCGTCTGCTTGGCAACCGTCTGATTTGATTACTTACACTTGATGATGATGATGATGATGGCGATGTTCATAGTTTGTGTCTTCGGTGGGGGGAAAAGTGTTGATAAGTTTCTCTCATGCCTCTGCAAAGCTTGTTATGTTGAATCTCCAAGAAAGGACATAGTTGGCAGAGAAGAGAATCTTTTAAATTAGTGAAATTACTTATAAGTGAGAAATCCTTCACGTTATCAACTGTTGGTTCCTTATTAATTTTTTAAATTTTTAG
TARGET=78,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5367
PRIMER_LEFT_PENALTY=0.053944
PRIMER_RIGHT_PENALTY=0.482784
PRIMER_LEFT_SEQUENCE=CTTTTTGTATTGCGTCTGCTTG
PRIMER_RIGHT_SEQUENCE=GCCAACTATGTCCTTTCTTGGA
PRIMER_LEFT=27,22
PRIMER_RIGHT=204,22
PRIMER_LEFT_TM=59.946
PRIMER_RIGHT_TM=60.483
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.6734
PRIMER_LEFT_1_PENALTY=0.643136
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=CCTTTTTGTATTGCGTCTGCTT
PRIMER_RIGHT_1_SEQUENCE=CCACCGAAGACACAAACTATGA
PRIMER_LEFT_1=26,22
PRIMER_RIGHT_1=125,22
PRIMER_LEFT_1_TM=60.643
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=1.1259
PRIMER_LEFT_2_PENALTY=0.643136
PRIMER_RIGHT_2_PENALTY=0.482784
PRIMER_LEFT_2_SEQUENCE=CCTTTTTGTATTGCGTCTGCTT
PRIMER_RIGHT_2_SEQUENCE=GCCAACTATGTCCTTTCTTGGA
PRIMER_LEFT_2=26,22
PRIMER_RIGHT_2=204,22
PRIMER_LEFT_2_TM=60.643
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=1.1504
PRIMER_LEFT_3_PENALTY=0.053944
PRIMER_RIGHT_3_PENALTY=1.096426
PRIMER_LEFT_3_SEQUENCE=CTTTTTGTATTGCGTCTGCTTG
PRIMER_RIGHT_3_SEQUENCE=CTGCCAACTATGTCCTTTCTTG
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=206,22
PRIMER_LEFT_3_TM=59.946
PRIMER_RIGHT_3_TM=58.904
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=1.1545
PRIMER_LEFT_4_PENALTY=0.053944
PRIMER_RIGHT_4_PENALTY=1.100593
PRIMER_LEFT_4_SEQUENCE=CTTTTTGTATTGCGTCTGCTTG
PRIMER_RIGHT_4_SEQUENCE=TCTCTTCTCTGCCAACTATGTCC
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=214,23
PRIMER_LEFT_4_TM=59.946
PRIMER_RIGHT_4_TM=59.899
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_01476 Ps_contig_mira-and-tgicl-ass_franssen_14409
SEQUENCE=AGTAGTTCTGAGCTGAGCAGACAAAGGAAAAGTCAAGTCAATGAAGAATCAAGCAAAAGACTATTCTACTTCAATTAAACTATGAGCTTTGTTATGTTGACACCAAATTTCCTATATCTTAATTTTTTTTCTAAAATATTTTAAGTAGTTACACAAAAACCAAANACAAGAAAAATAATTAAATAAATAAATA
TARGET=180,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01477 Ps_contig_mira-and-tgicl-ass_franssen_14431
SEQUENCE=GACCTTTCTCTCTCTCTTTCTCTCTTTCTCATAACTAGACACATATTTTGGTTTGTARTMRKGGGTWGAAGAAAGTGTTCACATTGTGGTAACATAGGACATAATTGTAGGACATGCACATCCTTCACCACAAAGCTTATAGGACTTCGTCTCTTTGGTGTCCAACTATCCTCATCATCATTATCATCATCTAATACCATCATCAAGAAAAGCTTTAGCATGGACACTTTTCCCTCACCATCCTCTTCATCTTCTTCATTCTCTTCATCAACCATTCCTGATAATCATCATCA
TARGET=7,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01478 Ps_contig_mira-and-tgicl-ass_franssen_14438
SEQUENCE=GATTCTCGTTCGTTCATTCCTTTAGCGTTTTTTGTTTGAATCCAATCCAATCGAAGAAGAAGAAGATGGGAAACAATCTGGTGAGAAATTGAGTCTGGGGGAAGTGATTAGCGGCGAAAGTCAGGTTGTGGCTGGAACCAATTATCGACTTGTGTTGGCGGCAAAAGATGAATCTGCTGCTACCAAGAATTATGAAGCGCAAGTTTGGGATAAACCTTTTGCCCATTTTAGGAACCTCACTTCGTTTAAACCACTTAATTAATCATGCTTTTTTGTAATATTGCAAAATTGTTATATAATGTGTTGTTGAATTTTCAATAATAATAAGGTTTTGATTTTAAGAAACG
TARGET=53,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2622
PRIMER_LEFT_PENALTY=0.256503
PRIMER_RIGHT_PENALTY=0.005674
PRIMER_LEFT_SEQUENCE=CTCGTTCGTTCATTCCTTTAGC
PRIMER_RIGHT_SEQUENCE=CGAAGTGAGGTTCCTAAAATGG
PRIMER_LEFT=4,22
PRIMER_RIGHT=243,22
PRIMER_LEFT_TM=60.257
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.3338
PRIMER_LEFT_1_PENALTY=0.328094
PRIMER_RIGHT_1_PENALTY=0.005674
PRIMER_LEFT_1_SEQUENCE=GTTCGTTCATTCCTTTAGCGTT
PRIMER_RIGHT_1_SEQUENCE=CGAAGTGAGGTTCCTAAAATGG
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=243,22
PRIMER_LEFT_1_TM=59.672
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=0.4074
PRIMER_LEFT_2_PENALTY=0.401769
PRIMER_RIGHT_2_PENALTY=0.005674
PRIMER_LEFT_2_SEQUENCE=GATTCTCGTTCGTTCATTCCTT
PRIMER_RIGHT_2_SEQUENCE=CGAAGTGAGGTTCCTAAAATGG
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=243,22
PRIMER_LEFT_2_TM=59.598
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.5028
PRIMER_LEFT_3_PENALTY=0.256503
PRIMER_RIGHT_3_PENALTY=0.246250
PRIMER_LEFT_3_SEQUENCE=CTCGTTCGTTCATTCCTTTAGC
PRIMER_RIGHT_3_SEQUENCE=GCGCTTCATAATTCTTGGTAGC
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=199,22
PRIMER_LEFT_3_TM=60.257
PRIMER_RIGHT_3_TM=60.246
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=0.5743
PRIMER_LEFT_4_PENALTY=0.328094
PRIMER_RIGHT_4_PENALTY=0.246250
PRIMER_LEFT_4_SEQUENCE=GTTCGTTCATTCCTTTAGCGTT
PRIMER_RIGHT_4_SEQUENCE=GCGCTTCATAATTCTTGGTAGC
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=59.672
PRIMER_RIGHT_4_TM=60.246
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_01479 Ps_contig_mira-and-tgicl-ass_franssen_14445
SEQUENCE=ACCCACCAAAATACATTTCTTTCTTAACCTAACCCACTAATCTTTAAACAAGTACTATGAACACAGACATCACAGAACGACTGTTTCAACTAAAACTTAAGTACATGTTACAACTGTTTTTTTATTTATTTGCATAACCGTACACGTTAAGATCTATTAGGACGAAGCGTATCAAATGGCCTATGCTGAACCGTTGGATACACGGGAAATGTACGACTATGATTTTGAACCATAGGATGACGACGCGTGGCAGCATCGACGGCAGATGTTGGAACAGCGACACGCTCACAAGAAGGGCACATGGTAAGCGTTGTAGGCGGTGTCATTTGCATGTAGAATTGTGGGGAAAGTTTCAGTGCTCTAAGCTCTTGAACTTCTTTTTGCAACCGCCGATTTTCATCCGTTAGATTCTCACAACATCTCTTCAAAAACTCACAATCTACCTCCGTTTGTTTCAGCTTAGTCCTTGCTCTACGATTTTGAAACCACACTTCAACTTGTCTTGCACGAAGGCCTAATTGTTTTGCAAGTGCCAATTTTTGTTTGGGGTTAAGAGTGTTGTGTTCTTTGAAACTTTCTTCAAGGATAATAGATTGGTCTTTGGTGAGTCTGAGTTTTTTTCTGGAAGTTTCAGCGTCTTCTTCATCGCTGATTCCTCTTGAACAATCTCTCTCCATGTCAAGATCTTCGCCGGTGATTTCTCTTTCGCTTCGTTTACCGCTCACACTTGAAACCGTGCTGTTGGGAGATGAGACGCCTGCTTCTTCTTCGCAGTTAGCGTCGGAAGGTAACCGGTTCACGTCGATTCCTCGGAGGAGTAACGGTCGTTCTTCGCCGCCTCTGCATGGTTCTGAATCCCGATCTGAAGATGTGAAAGCATTGATCCAAGAAGGTTTGTTGTGAGGATTGTAGGTGGAAGGAGAAGAGGAATGAGTTGAAGAAGACATGAGATTAAGGTATTGAGGTTTGGGTGGATTTTGAGGGAAGTTTAAGCTTAGGCTCAAACCCAAATCTTCCTTTTCAACTGTCATGTTTTGTAACCTTGTAAGACAAGAAAGAAAACTAAAGTGTTTTGTTTTTGGGAACTAAGAAACTGAGTTTTTTTGAGTTACTTGGGAGAGAAATTGGGGAAGAGAAGAGTGAAAGAGAAGAAGCTTTTTGAAAAGAGAGAGAGAAATGGGAGAGATGAATACCAHCGWSGAGAGGGAAGTTGTGGGAA
TARGET=1165,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01480 Ps_contig_mira-and-tgicl-ass_franssen_14446
SEQUENCE=CAGAGACAAACAAACAGACCAAAGGACAAATGCATGAAGGTATGAAGACCAACCTAACGAAATGGAGATGTATTCCAGTAGAAAAAGGTAACCCTTGAWTTCCTTTTCTTTTATTCCTTTGATTGTCCTTTTCTTTCTTGTTTTTTCATTTGATCACTTACTACTTCTCTTTTCTTTGAATACAAGGTCCTTACTAGTCAAGGATGAAGGCTAAGCTTAGAGAAATCAGAGTTTCAATTTGAAACCCTAATCCTGCTTTGATTCAAGCTCTGGTGTGGGATTGGAATTGCAGCTACAATAGTRAAAGTTTTTTTTAGGGTTCTTCTTTTTTCATTCCTTGTTCATCTGATTTCTTCTGTTATTTTTTAGGTTTTTTAGGTATAGTATTTTAATTGATTGATTGTTATTTTAGGTATTTTTATATTTGTATTTTTTAA
TARGET=392,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01481 Ps_contig_mira-and-tgicl-ass_franssen_14447
SEQUENCE=GAATACAAGGTCCTTACTAGTCAAGGATGAAGGCTAAGCTTAGAGAAATCAGAGTTTCAATTTGAAACCCTAATCCTGCTTTGATTCAAGCTCTGGTGTGGGATTGGAATTGCAGCTACAATAGTAAAGTTTTTTGGTCTTCTTTTTTCATCCTTTGTTCATCTGTATTTCTTCTGTTWWTTTTTAGGTTTTTTAGTATAGTATTTTTAATTGATTGATTGTTATTTTAGGTATTTTTATATTTGTAKTTTWTAGAAATCGTTATTTTTTANACTTAAGTTGGTATTTTGATTNTGTATTTAGTTAAGTTTACTTTGACTTAAGTTTAATTCTAGTATTTTAA
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01482 Ps_contig_mira-and-tgicl-ass_franssen_14448
SEQUENCE=ATGAATCCTTGCTTGGTAGAGCACTTCATCTGCAACCCAATTTCCAATACCTGAAATATAGCTTTGATCTAGTAATAAAGCCTTGATTTCAGTTTTCTTCTTGTGCAAACGCCCAGTGAATTCGTCAAGTGTCATGGGTTCAAAGAGTGCATCAGGACCAAGCTCGGATATGGGAGGCACTGATGTGGGATCTTTGAGCAGGCGAACTTTTGCAAATCTCCTTTTGTCAGTGAAAGATATCTCCAAACCATCATCTAGCTGAATAAAAAACTTCGAATATTTGGAAGGCCATTCATCCTCATCATTTACAGCAGACCTTTTGTACTTTGTTACTGCAACTCCCTTGATGTATATCGCACCAGCCATACCGAATTGGAAGGAAGGAAACGGCGGAGAATCGAGCTGAAGCCACATGTTCTTGCCCTTCCGGCGAGCTGCGACAATGGTTTTGCCGATGACGGAAGCTTCAAATTCGGAGTGAGAAACACCGTCGATGACTTTTGAATCATCGGCAACGATGCACTTGGTTATCTTCTTCCCGATACAGTTCTCTTCCACCGCTTTTCTTGCCGCCTCTACCTCCGGTAGCTCCGGCATTGTGCTTGCTCGCTCTGATTGCTATCTATGGAGTTTTCCCGCGTTGCTTCTCTTCTTACAAGTACGCTACTCTAHCSMCGCC
TARGET=375,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01483 Ps_contig_mira-and-tgicl-ass_franssen_14451
SEQUENCE=TTCCTCAAGCCACCCACTCCTTTCGTATCCTGCACTCTTCATTCCATTTTCGTCCTTTGAAAATTCCAGACGGATTCTGAGTTCTTCATTCTCTCAAAGTCTCACCTCCATCATTTCATATTCCACAATCATTCCGCGAGGACTTTGCAAGCGAAACTTCCTCTTGCATCCGAATTTGTTCTTGCTGCAACTAGGCAGCTTTGCTCTGCAATACGAGTTTTCCCTTTCTGCAGCTGGAAGTTCCAATTCCACAGTTTGGATCTTGACGCGGCAAGGCGATATTCTCACTTTCTACCTAACAAACATGGAAGTCAGAAGAAGTATAACAGGAAGCAGAAAGAAACAAGATTTCGATACTTATGCAGAGATTGTACACGACTCCATAATCAGAATAAATCGCGACTTCGAAGATTCAATTTGGTTAGGGAGGTCGGGGTTGAGGAGGAGATCGATTTGTCGAAGCCGAAGACGAATCTAAGTAACAACAATTTCATATTCGATTGGCCTTTGGTGTTCGAGACAAGTACAAGATCCATTTCCGGCCATGTTCATGAACAGGAACCGATCAAGAAGAAGAAGAATCTCACAGAAAATCGCATCAATAATCGCATCAATAATCGCAGATTCATACACACGTAGTTTCAGGTCAATAAGTATTCCTCATTTCTGATCTCTTTCTTTTGATTCGACGCGTTTGCAAGTCGTTACTGGTTTCGTGATTAGACGGTCTTTGGGGTCATCATGATTCATCACCGATAGCGCGCAGTAGATGGAAGATACTGGGATCCGACGCGATGAAGCTTCACGTTTTGGACATCTCGAAGACCGGCACATCAAATTTATTCGTCGTCCTTGATTCTTCATTCGACGTCCTTTCCGAGAGAGAACACGAGACGCGGATGGTGGATCGAGGTGAGGAAGCTCGTCGCGGCGAGTTGTCCCCG
TARGET=568,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4904
PRIMER_LEFT_PENALTY=0.418705
PRIMER_RIGHT_PENALTY=0.071684
PRIMER_LEFT_SEQUENCE=GTTGAGGAGGAGATCGATTTGT
PRIMER_RIGHT_SEQUENCE=ACCGTCTAATCACGAAACCAGT
PRIMER_LEFT=435,22
PRIMER_RIGHT=727,22
PRIMER_LEFT_TM=59.581
PRIMER_RIGHT_TM=59.928
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.6220
PRIMER_LEFT_1_PENALTY=0.418705
PRIMER_RIGHT_1_PENALTY=0.203343
PRIMER_LEFT_1_SEQUENCE=GTTGAGGAGGAGATCGATTTGT
PRIMER_RIGHT_1_SEQUENCE=TCACGAAACCAGTAACGACTTG
PRIMER_LEFT_1=435,22
PRIMER_RIGHT_1=718,22
PRIMER_LEFT_1_TM=59.581
PRIMER_RIGHT_1_TM=60.203
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=0.8926
PRIMER_LEFT_2_PENALTY=0.418705
PRIMER_RIGHT_2_PENALTY=0.473868
PRIMER_LEFT_2_SEQUENCE=GTTGAGGAGGAGATCGATTTGT
PRIMER_RIGHT_2_SEQUENCE=AAGACCGTCTAATCACGAAACC
PRIMER_LEFT_2=435,22
PRIMER_RIGHT_2=730,22
PRIMER_LEFT_2_TM=59.581
PRIMER_RIGHT_2_TM=59.526
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=1.0115
PRIMER_LEFT_3_PENALTY=0.162220
PRIMER_RIGHT_3_PENALTY=0.849238
PRIMER_LEFT_3_SEQUENCE=CGAAGATTCAATTTGGTTAGGG
PRIMER_RIGHT_3_SEQUENCE=GCGTCGAATCAAAAGAAAGAGA
PRIMER_LEFT_3=405,22
PRIMER_RIGHT_3=691,22
PRIMER_LEFT_3_TM=59.838
PRIMER_RIGHT_3_TM=60.849
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=1.0224
PRIMER_LEFT_4_PENALTY=0.950696
PRIMER_RIGHT_4_PENALTY=0.071684
PRIMER_LEFT_4_SEQUENCE=GAAGCCGAAGACGAATCTAAGT
PRIMER_RIGHT_4_SEQUENCE=ACCGTCTAATCACGAAACCAGT
PRIMER_LEFT_4=458,22
PRIMER_RIGHT_4=727,22
PRIMER_LEFT_4_TM=59.049
PRIMER_RIGHT_4_TM=59.928
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_01484 Ps_contig_mira-and-tgicl-ass_franssen_14455
SEQUENCE=CACCTCCTCCAGTAGGGGATAAAAATACTTTTTGGAAGACCGGGTGGGTTTCTGAGGAACGTTCATACACTGAGTTATGACTTAAATGCTGAGAGAAATTTTGCGAAATGTTCCGTTGATGATGATGATACACAGAATGCGAAATCCTTCAATGAAAAGGAACGTGAGATCAATGATCCTTTAACTCCAAATAAAGAGGAAATGGAACATCTTAAAAGGTTGAATTTACTGGATCACCGTGAGGTTCCTGGGACAGGATTGGATGCCATTACG
TARGET=117,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3346
PRIMER_LEFT_PENALTY=0.195462
PRIMER_RIGHT_PENALTY=0.139129
PRIMER_LEFT_SEQUENCE=ACCTCCTCCAGTAGGGGATAAA
PRIMER_RIGHT_SEQUENCE=CACGGTGATCCAGTAAATTCAA
PRIMER_LEFT=1,22
PRIMER_RIGHT=240,22
PRIMER_LEFT_TM=60.195
PRIMER_RIGHT_TM=59.861
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.4385
PRIMER_LEFT_1_PENALTY=0.195462
PRIMER_RIGHT_1_PENALTY=0.243031
PRIMER_LEFT_1_SEQUENCE=ACCTCCTCCAGTAGGGGATAAA
PRIMER_RIGHT_1_SEQUENCE=ACCTCACGGTGATCCAGTAAAT
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=244,22
PRIMER_LEFT_1_TM=60.195
PRIMER_RIGHT_1_TM=59.757
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.7873
PRIMER_LEFT_2_PENALTY=0.648142
PRIMER_RIGHT_2_PENALTY=0.139129
PRIMER_LEFT_2_SEQUENCE=CCTCCTCCAGTAGGGGATAAAA
PRIMER_RIGHT_2_SEQUENCE=CACGGTGATCCAGTAAATTCAA
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=240,22
PRIMER_LEFT_2_TM=60.648
PRIMER_RIGHT_2_TM=59.861
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.7881
PRIMER_LEFT_3_PENALTY=0.195462
PRIMER_RIGHT_3_PENALTY=0.592680
PRIMER_LEFT_3_SEQUENCE=ACCTCCTCCAGTAGGGGATAAA
PRIMER_RIGHT_3_SEQUENCE=CGGTGATCCAGTAAATTCAACC
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=60.195
PRIMER_RIGHT_3_TM=60.593
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.8912
PRIMER_LEFT_4_PENALTY=0.648142
PRIMER_RIGHT_4_PENALTY=0.243031
PRIMER_LEFT_4_SEQUENCE=CCTCCTCCAGTAGGGGATAAAA
PRIMER_RIGHT_4_SEQUENCE=ACCTCACGGTGATCCAGTAAAT
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=244,22
PRIMER_LEFT_4_TM=60.648
PRIMER_RIGHT_4_TM=59.757
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_01485 Ps_contig_mira-and-tgicl-ass_franssen_14460
SEQUENCE=TAGGATTTGTCTAGATAGAATAACCATCAGGAACAGTCCAGGTTTTGTTCACCTGACAATCAAGCTAATACTCCCCAATTTTCGGTAGACATTTAAATCTGATACCTTGAATAATGGCGATGTGATTAGTACCTTGTAYKGRTGCAGAGATTGGAGAAGCAGATCACGATGAGCATAAATCTGTAGATCCGTTGGATAAATTTCTTCCTCCTCCACCCAAAGTTAAATGCTCAGAGGAATTACAAAGAAAAATAAATAAATTTCTGGAGTATAAGAAGGCTGGAAAAAGTTTTAATGCAGAAGTTCGCAACAGGAAGGACTATCGAAACCCAGACTTCTTACTGCATGCTGTGAGGTACCAGGATATTGATCAAATAGGGTCTTGCTTTAGTAAAGATGTATTTGACCCTCATGGATATGATTCAAGTGACTTCTATGATCAAATAGAAGCTGATATGAGGCGGGAAAGTGATAGGAAAGAGCAAGAGAAGAAGAAACCACAAAACCAGAAGGTTGACTTTACTTCTGGTGGTACACAGCCAGGAATTGTGCTTGGTGCTCCCAGGATCAGCATGCCTGTTACAGGCGGTTCTGCTGCAACTACAGGTGGTTTGCATTTGGTGCCTCCTCCAGCGGTATTCTACTAATCGGGATGGCAGACAGAATAAAAAATCAAAATGGGATAAGGTGGATGGTGAT
TARGET=250,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01486 Ps_contig_mira-and-tgicl-ass_franssen_14461
SEQUENCE=TCAGTTTCAAATTGAAGAATACTGTTGATTATTTGCAGTYACCAGTAACCATACATTTAGAAATAGCAGTAGCTGATTTTGCATCAAATCCAATAATCAATATTAGCATTGGCACAAAGATGCCACCACCACCAACACCACCAACACTTCCAAATGAAGCTCCAAAGACTCCAATCAAGGTACCAACAATGATTTCCCATCCAAATTCTATATTTGGCCAAATATGTTCATAACCTGGATGACTAGTAACTTTGGCATGGTGATATAAAGATTGTAATGATGATGATTCATTTTTGGTGGTTCTATTAGCACAAAAGATGAGAAGAACAAACAAAAGAATGAATGAAAACAAGACCACATTTCTCATGACCCTTCAATGATGGAAAACTAGCCAGCCTGGAAAGAAGTAAACTTTTGGTTCMAAAATAGGAGAGCAGAAATGGAAGATATATACTTGACTAAGTTTC
TARGET=123,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01487 Ps_contig_mira-and-tgicl-ass_franssen_14462
SEQUENCE=CCGTTTGGTATTGGTAGGTCTTTGGTAGTCTTCATTGGAGTGTTGGTGGTGTTGGTGGTGGTGGCATCTTTGTGCCAATGCTAATATTGATTATTGGATTTGATGCAAAATCAGCTACTGCTATTTCTAAATGTATGGTTACTGGTGCTGCAATATCAACAGTATTCTTCAATTTGAAACTGAGACATCCAACACTTGATATACCAATGATTGATTATGATTTGGTGCTTTTAAACCAACCTGTTATAATTCTTGGTATTAGTATTGGAGTTGTTTTAAGTGTAGTTTTTGCAGATTGGATGATTACTGTTCTACTAATCATTATCTTCATAGTTACATCAGTGAAGGCTTTCATCAAGGGTCTTGACACATGGAAAAAAGAAACCATAGTGAAAGAGGAGTTTGTGAAGCATTTAGAGTCCACTGCCACTAGCAGTGAAGATGTTGAATACAAGTATCTTCCTAGTTGTCCAGATGATGAGCCTCAGAAGAAAATCAATCAAGGTACCATTCTTAGCAACATTCAATGGAAGAAATTTGGACTTCTTTGTTTTGTTTGGATTGCAGTTCTTCTCTTGCAGATTGCCAAGAATTATACAGCTACATGTTCAATCAAATACTGGTTACTAACTTTGTTACAGATACCAGTTACTGTAGGGGTTTCAATGTACCAAGCAATAGGTCTATATCAAGGGAAGAAGATTACAGTTTCTAAGCAAGATCATGGCACACATTGGCCTTTGCATCTTCTGATTGTATCACTTTCATGTGCTTTGCTAGCTGGAATATTGGGTGGACTTCTTGGTGTAGGTAGTGGATTTGTCATGGGTCCTTTGTTTATCGAATTCGGAATTGCGCCTCAGGTAGCAAGTGCCACGGCCACATTAGGAATGACATTCACAGCATCTATATCGGTTGCCCAATATTTATYTTCTCTTAGTAACTTTCCGGTTTTTCCCGGTTTACCTTTAAGTTAAGGTTAAAAATTGAAT
TARGET=53,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01488 Ps_contig_mira-and-tgicl-ass_franssen_14470
SEQUENCE=TGTGATGATTGGTTTCAATCTCTTAATATCAGGAAGGAAAAATGGTGGGATGAGAAGAAAAATTGATGTCATTTTCCTTTATCTCCAGCTCTGCTATGTGCTACATTGTAAAGTGAAACCTTCTGTCCTGATGGAGCTGGTGTGGGAACTTCTACAAGATGAAACAAATTCCGAGGATTTGTCAGTTTCGGATTGTAACTTTGGTTGCAAAGTCGATATGCGCTTCAGATTGTATTCATGATCAACTATTTCATTTGACTGGCTTTTTGTGTTTCTCTTTATTCCGATTCGCCAGAAAACATCTTCACGGTTAACCAAGTGTTTTTCTAGAAGAATCTCTTTCTTATATCCAGTACTAATACCCCCACCACCAGCTGGTCCATTTGTATACAATGCCGTAAACTCTCTCGTAAATTGGATAGCGTGTTCTTTCTGCTCAAAAAGTCCGTCCATGCGTAACCTAATATCTTCACTAGTCCTCTGTTGTGAAGCATTTTCGTCGCTGCTGAAAGCTTTGAGGCTATCATACCCAATCATATAAGCAACTATATGGTGATTAAGACCAGGAATTATTTCTTCCATCCATGATCTGACCACAAATTCAGCCGCTTTGGCACGTTTAACACATTCATAGCCTCCATATGATATCTCTCCCCATCCTTTCCAACCACAATCCTTTGGAACTAACTGTAAAAGTTTATCAGGAACTGATATAGTAGATGGTTTTGCCCCAAAACAAAGTACTCTACTGCTTGATAATGGAAGGAAGGAAACATCYTGAAAGTCAATGACTACATCAGGAGTCACATAAGCACTGGGGT
TARGET=761,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01489 Ps_contig_mira-and-tgicl-ass_franssen_14473
SEQUENCE=TGTGGATTCTGGTTAGACAAGGCTTTGAAATATCTTGGACTCAATATTGATGATGTTGATACAAATGATTCTTCTTCCACTGAGGCGATATTGGAATCATCTTCTCCTCCTCCTCCTCTACCATTGCAGCTTCAAGAGGGAATTGGCAAAGTGAATTTGATCATGACAGATTATTGCATGCCTGGGATGAGTGGCTATGATTTACTTAAAAGAGTCAAGGGATCTTCTTGGAAAGATGTTCCAGTTGTGATCATGTCTTCAGAAAATGTACCTTCCAGAATTACCATGTGCTTGGAAGAAGGTGCAGAAGAGTTTCTGTTGAAGCCTCTTCAGATATCAGATTTGCAGAAGCTTCAGCCTTATTTTCCTAAATCTCTTGATAATTCTTCGGATGAACAAGAATCTACTAACAGTACTTCTATAGATTCTTCTGACAATGAAGATGTGATCAACAATAATAACAGTAATGGGATTAGCAAAAGGAAGGCCATGTCTCCTGAGCCTCCGGAGAGATCAAAGCCAAAAATGAAAGGGTTAACGGTGGTGGTGTAAAGAGAAAACAGTACAAAATAAGAGTTGATGATGATGTTGTTGTCGCATTATTTAATTTAGTTTATGGTTATGGTTATATTTTGTTTCTATTAACNGAAGNGTCCTTTAAATGAATTCGCTTG
TARGET=104,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0079
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=GTGGATTCTGGTTAGACAAGGC
PRIMER_RIGHT_SEQUENCE=TTCCAAGCACATGGTAATTCTG
PRIMER_LEFT=1,22
PRIMER_RIGHT=296,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=0.1339
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=TGGATTCTGGTTAGACAAGGCT
PRIMER_RIGHT_1_SEQUENCE=TTCCAAGCACATGGTAATTCTG
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=0.2534
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.249074
PRIMER_LEFT_2_SEQUENCE=GTGGATTCTGGTTAGACAAGGC
PRIMER_RIGHT_2_SEQUENCE=GCACATGGTAATTCTGGAAGGT
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=290,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=60.249
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.2569
PRIMER_LEFT_3_PENALTY=0.253332
PRIMER_RIGHT_3_PENALTY=0.003589
PRIMER_LEFT_3_SEQUENCE=CTTCTTCCACTGAGGCGATATT
PRIMER_RIGHT_3_SEQUENCE=TTCCAAGCACATGGTAATTCTG
PRIMER_LEFT_3=70,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=59.747
PRIMER_RIGHT_3_TM=59.996
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.2851
PRIMER_LEFT_4_PENALTY=0.281487
PRIMER_RIGHT_4_PENALTY=0.003589
PRIMER_LEFT_4_SEQUENCE=ATGATTCTTCTTCCACTGAGGC
PRIMER_RIGHT_4_SEQUENCE=TTCCAAGCACATGGTAATTCTG
PRIMER_LEFT_4=64,22
PRIMER_RIGHT_4=296,22
PRIMER_LEFT_4_TM=59.719
PRIMER_RIGHT_4_TM=59.996
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_01490 Ps_contig_mira-and-tgicl-ass_franssen_14477
SEQUENCE=GTGACCAAAAAGATGGAGTTACTTTATCTTCTATTCTCTATAGTCTTCACTTCTTTCACGACTCTTATCCTATCCCTCATTCTCCCTTTTCACGCGCTACTGCGTCGTTTAGTATCCTCACGCGCTTCTTCTTCTTCTTCCAGTGACGAAGCTGAACCCATTTCTCTCTATGAAGGTACAGTCTATCATCAGCGCCGTCACCCAGTTCACCACTCCTTTCAGTATCGTGTTCGCTACGCGCTCATTGACATCGACCGTGCACCTCACGCGCCGCCCAATCATCTTTCTCCCGATGAAGCTCGTCGAATTACCGGTACCAATGGAACCATTTTGCTTTTGACGATTCCACCCAGTGTGGGATATGAACAGAATCCATTGAGTGTGTATTATTGCTATGATGTTGAAGAGTCTGCCACATGTTTGAAGAAATGCATTGCTGAGGTAACAAATACACCATGGGCTGAAAGAGTAACCTTTATTTTCAATCCACATTCTGATTTAGTGGCCAAAGCTTTGCATGTCAGTCCTTTTATGGATATGCTTGGAAGTTGGAACATTAAAGCAAGTGATCCCGGAGATTATCTTAGCATATCAATTTCAGTTCATCACCCTGAGTTTGGAAACTATTTTACTGCTAGTTTGAAAGCCAAAAAGCTGCACTCGTCATCAGCGTCAGATCATGCAGTTTTCTTTTGGTTGATGCCTCATAAGGTTGCCGTATGGATATATTGGCATGCCATAAAACTGTGGTGGAAAAATGTGCAGTTTATACAACACCCTAGATATGCGACTCCGACGTACAGAGACGAAGCTTTAATACGAGATAGAAATCTGCAGTGTTGTGGATTCAGTGCAGAAAGAGAAAACAAGAAGGACTGTTTATCAYCTGAAGCAAGTCCTAGAGACAGGTGGTTTAGATGGATCGATGMTAAATGGCCTATTGGTCAATAAAGAAAAATAAACAAGCTATTTT
TARGET=125,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01491 Ps_contig_mira-and-tgicl-ass_franssen_14479
SEQUENCE=TCGGGGTGTTAATGTGTAACRGTAAGTCAGTAACAGAGTCTCCCTCTTCATTGGATAGAGCGAAGCAGCAGAAGCGGAAGCAATGGACATATCATTTCCCGCCGCCTTCAAATCCCTCAACTTACCACCATCTAACGCTTTTCATTTTCGTTCCAAACTTYYTCTTTTCGTTCAAACTATCAATTTACTTCACTTCAAACCCTACCMTWTCTTCTTTCTTCKTCTTCTTCTTCTTCTCGCCTCTTTCCCTTCCTTCTCCTCTCTCACAA
TARGET=223,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01492 Ps_contig_mira-and-tgicl-ass_franssen_14480
SEQUENCE=GGACTTATAAGTGTCTGTGAAASTRYGTTCGGGGGGTAACAGTAAGTCAGTAACAGAGTCTCCCTCTTCATTGGATAGAGCGAAGCAGCAGAAGCGGAAGCAATGGACATATCATTTCCCGCCGCCTTCAAATCCCTCAACTTACCACCATCTAACGCTTTTCATTTTCGTTCCAAACTTTCTCTTTTTCGTCAAACTATCAATTTACTTCACTTCAAACCCTCCATTTCTTCTTCTTCTTCTTCTTCTTCTCGCCTCTTTCCCCTTTCCTTCTCCTCTCTCACCAATGGTTCTGCTACTGCTACTGCTGCTGCTGAGATAAGCCAGGAAGGTACTTTGCCTCATGGTGTTGGAGAGGGAGTTAATGAAGCCGCACATCCTAAGATTCTTCAGGTTGTGCTCGTTTCCCCTCAGATTCCAGGAAACACTGGCTGCATTGCTAGAACATGTGCAGCATCAGCTGTTGGACTACACTTAGTTGGGCCATTGGGATATAAGGTGGATGATACTAAACTAAAGCGAGCTGGATTGGACTACTGGCCATACGTGGTTGTTAAAATTCATGATTCTTGGGAAGGTTTTCGTGATTATTTTTTGCAACAGGAGGGTGAAAAGCGATTGCTAGCATTTACAAAAAGAGGAACAAAAATTCATTCTGATTTTTCATACAGAAAAGGCGATTATCTATTATTCGGTGCTGAAACGACTGGTCTTCCGCCTGAAGCTTTGTTAGACTGCAAAACTCAACCGTTTGGCGGTGGGACTATTAAGATCCCGATGGTTGAAACATACGTCAGGTGTTTGAATCTATCGGTAAGTGTTGGCATAGCTTTGTATGAAGCTTCTAGGCAATTAAACTATGAATCACTTCAGATACCTTCAGAATCATGTATTGATACTGAGGAGGAATCATTCATTACTGAAGACATTTTTGGATGATCATTATATAATTTATTTTGTTTCAGTACAGAGAATGTTCCTGTGTTTTTTTCTTAATTGATTATGCTTAATTGAACATGCCTGGCCTGAAACCTGATCAAGGGTGTAGTTCTAGTAAAGGGAGCATTTAGAAGCTGAGTCTCTAATTTTTMAAACTATTCTGACAAATACAGTTGTTTAACTTTA
TARGET=228,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01493 Ps_contig_mira-and-tgicl-ass_franssen_14480
SEQUENCE=GGACTTATAAGTGTCTGTGAAASTRYGTTCGGGGGGTAACAGTAAGTCAGTAACAGAGTCTCCCTCTTCATTGGATAGAGCGAAGCAGCAGAAGCGGAAGCAATGGACATATCATTTCCCGCCGCCTTCAAATCCCTCAACTTACCACCATCTAACGCTTTTCATTTTCGTTCCAAACTTTCTCTTTTTCGTCAAACTATCAATTTACTTCACTTCAAACCCTCCATTTCTTCTTCTTCTTCTTCTTCTTCTCGCCTCTTTCCCCTTTCCTTCTCCTCTCTCACCAATGGTTCTGCTACTGCTACTGCTGCTGCTGAGATAAGCCAGGAAGGTACTTTGCCTCATGGTGTTGGAGAGGGAGTTAATGAAGCCGCACATCCTAAGATTCTTCAGGTTGTGCTCGTTTCCCCTCAGATTCCAGGAAACACTGGCTGCATTGCTAGAACATGTGCAGCATCAGCTGTTGGACTACACTTAGTTGGGCCATTGGGATATAAGGTGGATGATACTAAACTAAAGCGAGCTGGATTGGACTACTGGCCATACGTGGTTGTTAAAATTCATGATTCTTGGGAAGGTTTTCGTGATTATTTTTTGCAACAGGAGGGTGAAAAGCGATTGCTAGCATTTACAAAAAGAGGAACAAAAATTCATTCTGATTTTTCATACAGAAAAGGCGATTATCTATTATTCGGTGCTGAAACGACTGGTCTTCCGCCTGAAGCTTTGTTAGACTGCAAAACTCAACCGTTTGGCGGTGGGACTATTAAGATCCCGATGGTTGAAACATACGTCAGGTGTTTGAATCTATCGGTAAGTGTTGGCATAGCTTTGTATGAAGCTTCTAGGCAATTAAACTATGAATCACTTCAGATACCTTCAGAATCATGTATTGATACTGAGGAGGAATCATTCATTACTGAAGACATTTTTGGATGATCATTATATAATTTATTTTGTTTCAGTACAGAGAATGTTCCTGTGTTTTTTTCTTAATTGATTATGCTTAATTGAACATGCCTGGCCTGAAACCTGATCAAGGGTGTAGTTCTAGTAAAGGGAGCATTTAGAAGCTGAGTCTCTAATTTTTMAAACTATTCTGACAAATACAGTTGTTTAACTTTA
TARGET=305,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01494 Ps_contig_mira-and-tgicl-ass_franssen_14494
SEQUENCE=CATGGCTAGTAGTAGTAGTAGTGGTACTAGTAGCTTCCAAAACACGCCTCCTCAATCTCAACCTTGTTTTATGACTAGAATGGCCATGAGAATATCAAGATCAAGATGGTTCATTTTTTTTGTAGAAGAGTGTTTCATTACCAAAACGGACCAAGATCTGATCTTGGTTCTAACCCTTTTAATTCTAGGACTTGGATGATGTTGGAGTTGGTTGCTTTGTTGGTTCAAATTACAAGCACAACATTTACTTTGGTGGTTTCAAAGAGTGAGAAACCGGTTAGGCCTATGGAGGGGTTTTGGGTTGTTGGTTATGACAT
TARGET=7,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01495 Ps_contig_mira-and-tgicl-ass_franssen_14500
SEQUENCE=TCGGRKGGATTTTAGAAAATAGCAAAATAGGAGTGTGTTTAACGACATCGTATCCTAAATCAACCACCAGCACCCGTAGCAAGTTTCTAACCCTAGTTCATCACACGCGCCGCCGTCATTCTCTACTCCGGCCGGTAACATGGTACGTCGCAAACGGGAAAGTCTTCCACCGGMGATTTCAAGAGATGATTATCCTTCAAAGCGTAGAAGACATTCTATGTTCGAAATTTCGGCCTCTAATCACGGAGTATCTTCTTCTTCTAGAAAATGGGTTTTCTCCTCCGAAAATTGTTCTGATTGCACGGATACGATTGTAGTTGTTTCATACAATATACTTGGTGTTGAAAATGCATCAAATCATATGGATTTGTATTCCAATATTCCACGACGTTTCTTGGAGTGGGGAAGACGCAAGAGGCTTATACTTGACGAGATTAACGGCTATAATGCAAGTATACTCTGTTTTCAGGAGGTTGATCATTTTGACGATTTAGATGATCTTTTTCAGAACAATGGCTTCAAAAGTTCTTACAAGGCTCGCACAGGAGAAGCAAACGATGGATGTGCTATTATTTTGGAAAGACAAATTATTCAGTCTTTTGCATCAAGAAGATA
TARGET=251,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01496 Ps_contig_mira-and-tgicl-ass_franssen_14513
SEQUENCE=CAGAATCATCCGTAGGAGACCCTTCATCATCTTTTTCAGCAAACAAAATCTTCATCCTCCTCGTCACTTTCTATYTTCACCAGCTTCATTTCTGATGCGCTCAAGATGTGGATCAACAGCATCATCATCATCGCTCTCAAGAACCTTAGCCACACCAGCGGCTTGTTGGGCATCTCCTAAATTCATAATTTTCAAGCCCTTTGAACTGATAAAGCCATAACAAATTGTGGTACTCATTTCTCTGAATATT
TARGET=120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01497 Ps_contig_mira-and-tgicl-ass_franssen_14514
SEQUENCE=CTGTCTTATAAGGGGCTCATCCATGAAGTGTTCACCACAATATTACGTGGCTTGTCTGGGGCCAAAGTTACCAAGCCTGGAAAATTTAGGAGTTGTCAAGATGGTTATGCTGTAAAATCATCTTTGAAAGCTGAAGATGGAATTCTGTATCCCCTTGAGAAGAGCTTCTTCTTTCTACCTAAACCTCCCACTCTTATTACTCATGAAGAGATTGACTATGTGGAATTTGAGCGACATGCTGCTGGTGGTTCAAATATGCATTATTTTGACCTTCTTATCAGACTAAAATCTGAGCAAGAGCATCTCTTCCGTAATATTCAGAGAAATGAGTACCACAATTTGTATGGTTTTATCAGTTCAAAGGGCTTGAAAATTATGAATTTAGGAGATGCCCAACAAGCCGCTGGTGTGGCTAAGGTTCTTGAGAGCGATGATGATGATGCTGTTGATCCACATCTTGAGCGCATCAAGAAATGAAGCTTGGGTAAGAAAAATAGAAAGTGACGAGGAGGATGAAGTATTTTGTTGCTAGAAAAAGATGATGAAGGGTCTCCTACGGATGATTCGTGGGGCAG
TARGET=430,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3632
PRIMER_LEFT_PENALTY=0.116503
PRIMER_RIGHT_PENALTY=0.246726
PRIMER_LEFT_SEQUENCE=GCAAGAGCATCTCTTCCGTAAT
PRIMER_RIGHT_SEQUENCE=TTCATCCTCCTCGTCACTTTCT
PRIMER_LEFT=293,22
PRIMER_RIGHT=516,22
PRIMER_LEFT_TM=59.883
PRIMER_RIGHT_TM=60.247
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.3632
PRIMER_LEFT_1_PENALTY=0.116503
PRIMER_RIGHT_1_PENALTY=0.246726
PRIMER_LEFT_1_SEQUENCE=GCAAGAGCATCTCTTCCGTAAT
PRIMER_RIGHT_1_SEQUENCE=CTTCATCCTCCTCGTCACTTTC
PRIMER_LEFT_1=293,22
PRIMER_RIGHT_1=517,22
PRIMER_LEFT_1_TM=59.883
PRIMER_RIGHT_1_TM=60.247
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=0.5179
PRIMER_LEFT_2_PENALTY=0.116503
PRIMER_RIGHT_2_PENALTY=0.401361
PRIMER_LEFT_2_SEQUENCE=GCAAGAGCATCTCTTCCGTAAT
PRIMER_RIGHT_2_SEQUENCE=CGTAGGAGACCCTTCATCATCT
PRIMER_LEFT_2=293,22
PRIMER_RIGHT_2=557,22
PRIMER_LEFT_2_TM=59.883
PRIMER_RIGHT_2_TM=59.599
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.5589
PRIMER_LEFT_3_PENALTY=0.312177
PRIMER_RIGHT_3_PENALTY=0.246726
PRIMER_LEFT_3_SEQUENCE=GAATTTAGGAGATGCCCAACAA
PRIMER_RIGHT_3_SEQUENCE=CTTCATCCTCCTCGTCACTTTC
PRIMER_LEFT_3=377,22
PRIMER_RIGHT_3=517,22
PRIMER_LEFT_3_TM=60.312
PRIMER_RIGHT_3_TM=60.247
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=141
PRIMER_PAIR_PENALTY_4=0.5589
PRIMER_LEFT_4_PENALTY=0.312177
PRIMER_RIGHT_4_PENALTY=0.246726
PRIMER_LEFT_4_SEQUENCE=GAATTTAGGAGATGCCCAACAA
PRIMER_RIGHT_4_SEQUENCE=TTCATCCTCCTCGTCACTTTCT
PRIMER_LEFT_4=377,22
PRIMER_RIGHT_4=516,22
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=60.247
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=140
=
PRIMER_SEQUENCE_ID=SSR_01498 Ps_contig_mira-and-tgicl-ass_franssen_14518
SEQUENCE=CTCCTCCGGTACAATCAGAATCTCCACCTGTAACAAATCCAACCCCTCCTCCGCCGCCTGACTCCAGCTCCAGCTCCTACCCTCCTCCTCCTACCGAACCTWGACTCTAACCCTAACACTTCTCCTCCACCGTCAATTCAGTCTCCGCCTCCTCCTCCGGCGAGTGTAGCAGAGCCACCGACGAGAAGTACGCCGGGATCGGCGCTAGTGAGTCCGGGTCCTGTACATGCTATTTTCTAGGGTTCTTTGTTTGGCTATTTTTGGTTTTGTAGATCATTGAAGAAGGGAAATTGGTTGGTTGGTGTTGTGATTGATCTGATGTCTTTTATTTTATTATATTA
TARGET=81,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01499 Ps_contig_mira-and-tgicl-ass_franssen_14518
SEQUENCE=CTCCTCCGGTACAATCAGAATCTCCACCTGTAACAAATCCAACCCCTCCTCCGCCGCCTGACTCCAGCTCCAGCTCCTACCCTCCTCCTCCTACCGAACCTWGACTCTAACCCTAACACTTCTCCTCCACCGTCAATTCAGTCTCCGCCTCCTCCTCCGGCGAGTGTAGCAGAGCCACCGACGAGAAGTACGCCGGGATCGGCGCTAGTGAGTCCGGGTCCTGTACATGCTATTTTCTAGGGTTCTTTGTTTGGCTATTTTTGGTTTTGTAGATCATTGAAGAAGGGAAATTGGTTGGTTGGTGTTGTGATTGATCTGATGTCTTTTATTTTATTATATTA
TARGET=291,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01500 Ps_contig_mira-and-tgicl-ass_franssen_14519
SEQUENCE=CCAGCTCCAGCTCCTACCCTCCTCCTCCTCCGCCTGACTCTAACCCTAACACTTCTCCTCCACCGTCAATTCAGTCTCCGCCTCCTCCTCCGGCGAGTGTAGCAGAGCCACCGACGAGAAGTACGCCGGGATCGGCGCTAGTGAGTCCGGGTCCTGTACATGCTATTTTCTAGGGTTCTTTGTTTGGCATTTTGGTTTTGTAGATCATTGAAGAAAGGGGAAAATTGGTTGGTTGGTGGTTGTGGATTTGGATCTGATAGCTTTTTACTTTTTATTTAATTATTTACTGAGTNATTATGTTTTNATTGAGNTGGGNNTGGGA
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01501 Ps_contig_mira-and-tgicl-ass_franssen_14519
SEQUENCE=CCAGCTCCAGCTCCTACCCTCCTCCTCCTCCGCCTGACTCTAACCCTAACACTTCTCCTCCACCGTCAATTCAGTCTCCGCCTCCTCCTCCGGCGAGTGTAGCAGAGCCACCGACGAGAAGTACGCCGGGATCGGCGCTAGTGAGTCCGGGTCCTGTACATGCTATTTTCTAGGGTTCTTTGTTTGGCATTTTGGTTTTGTAGATCATTGAAGAAAGGGGAAAATTGGTTGGTTGGTGGTTGTGGATTTGGATCTGATAGCTTTTTACTTTTTATTTAATTATTTACTGAGTNATTATGTTTTNATTGAGNTGGGNNTGGGA
TARGET=225,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1206
PRIMER_LEFT_PENALTY=0.167063
PRIMER_RIGHT_PENALTY=0.953558
PRIMER_LEFT_SEQUENCE=CTAACACTTCTCCTCCACCGTC
PRIMER_RIGHT_SEQUENCE=GCTATCAGATCCAAATCCACAA
PRIMER_LEFT=45,22
PRIMER_RIGHT=260,22
PRIMER_LEFT_TM=60.167
PRIMER_RIGHT_TM=59.046
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=1.1386
PRIMER_LEFT_1_PENALTY=0.185055
PRIMER_RIGHT_1_PENALTY=0.953558
PRIMER_LEFT_1_SEQUENCE=CGCCTGACTCTAACCCTAACAC
PRIMER_RIGHT_1_SEQUENCE=GCTATCAGATCCAAATCCACAA
PRIMER_LEFT_1=30,22
PRIMER_RIGHT_1=260,22
PRIMER_LEFT_1_TM=60.185
PRIMER_RIGHT_1_TM=59.046
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=1.2101
PRIMER_LEFT_2_PENALTY=0.256503
PRIMER_RIGHT_2_PENALTY=0.953558
PRIMER_LEFT_2_SEQUENCE=CGGGTCCTGTACATGCTATTTT
PRIMER_RIGHT_2_SEQUENCE=GCTATCAGATCCAAATCCACAA
PRIMER_LEFT_2=147,22
PRIMER_RIGHT_2=260,22
PRIMER_LEFT_2_TM=60.257
PRIMER_RIGHT_2_TM=59.046
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=1.3122
PRIMER_LEFT_3_PENALTY=0.167063
PRIMER_RIGHT_3_PENALTY=1.145108
PRIMER_LEFT_3_SEQUENCE=CTAACACTTCTCCTCCACCGTC
PRIMER_RIGHT_3_SEQUENCE=GCTATCAGATCCAAATCCACAAC
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=260,23
PRIMER_LEFT_3_TM=60.167
PRIMER_RIGHT_3_TM=59.855
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=216
PRIMER_PAIR_PENALTY_4=1.3302
PRIMER_LEFT_4_PENALTY=0.185055
PRIMER_RIGHT_4_PENALTY=1.145108
PRIMER_LEFT_4_SEQUENCE=CGCCTGACTCTAACCCTAACAC
PRIMER_RIGHT_4_SEQUENCE=GCTATCAGATCCAAATCCACAAC
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=260,23
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=59.855
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_01502 Ps_contig_mira-and-tgicl-ass_franssen_14523
SEQUENCE=GAAGGGTATTGAACGGTAGGGTAGGGGCCTGTTTGGTGCTTTGTATTTTCATTTTGTAACGCRTYKRKGGCAGAAAACAGAAGGTGGAGGGTGGAAGAAAGAAAGAAGAGGTGGTGGGTTTAGAGAGAGAAAAGGGTGTTACAGTTGACCATAATGAAGCTTATATAATTACAACAACTCATCCATTATCTGATACTATATACTCTCTCTTCGGTTGCTTTTATTTTCAGTATTCATTTTTATTTAATTCCATTTATATATTCTTTTATTTTCATTCTTATAACCATTCAATTCAATTTATGAAACACAGTTACCAACTTGACCAGAAACTCCTGCTTTCTCCTCGTCTCTAGCTTTGGACTTTGGTTCTTGTTGAATATTGTATGATCAGATTAGTCTCTTTGATTGAAAGTAAACAAAATAGCGTTGGTTGTTGCGATTGGTAAATACACACAT
TARGET=95,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01503 Ps_contig_mira-and-tgicl-ass_franssen_14524
SEQUENCE=CGTAACAAAATGTAAATTGCAGATATTATTTACAACAACAGTAAATATTTGTTACTCTCCACGTAGAAAATAATATAGAAAAACAAATAAATATGAGCAATTTGTACAAGAAAATGGAGACCAGAAAAGAAACAAGGGGAAAGAAGCTGTTCAGTGAATATGGATTTCTTGGTAAATTCCAACATGAGTAACTGTAGTCATGGTCAGTGAGACATGTAATGTGGTGTAGCATCTTTCTCACAGATTGTATACAGAATGAATTTGGCCCACTAAAAGTAACCTTTTAAATATTTGTAGATTTGAAAGTCAATCAATATTCAAAACCATCATCAAACCAGTTATTCTTCAAGTATTTAGCAAGATTTTTTCGTTTGAAATGACCAAACTAGTCAAGTTCCTGATCAAATATCATCTCCTGATCACATATAGGACAGGAGTCACTTCTTTCCATCCACTCAAGAATGCAAGCCAAGTGAAAGTGGTGTTCACATTTGGTAAGAGTTTTAGGATTCTCAACATCATATTCTTCAAGGCAGATTGGACAGCCATCTTCTTCTTCTATCACCAGTGCTTGAGGTTTAATTGATTTGCACAGCTCTGCCTTGCTAGGAGATAATGGTGCAGATTTAGCTTGAGCTTTAGGGTCACATTCTTCAATATCTTCGCATGTAACTAAAGTTTCAAAACTACTACCACTAACTGTGTCTCTGCCTGACTTGGAGTCTGTCGATGCTGGACCTCCCAAGACTGTATCATAAGGGAGTGGTGCAGGAGGAGGCTGGTAAGTATCAGGTGTGGATTCTTCCACATTCAAGCCAACCAGCAATCCAGCAGTGAGTGAATCGGTCGGACCATCATTAGAGGTTAAAGAATCGCGCTCTTCTAAAATTGGTGGGCAATAGTAATACACTGGAGTTCCATGCAGATGCGTTTTTCTGGCAGAACAACAGCAACCTCCCATTTTTGAAGCAGTTCGTTTCAGTTATTTAATCAAACCTACCAGCAACACTCCCATTCTCTTCAATTATTTAAGATCAAGTGAGGATCAAAAGCCACCAAAATTTAACCATCGTTTACGGAAAGTGGAGATGTCAATC
TARGET=549,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0689
PRIMER_LEFT_PENALTY=0.020747
PRIMER_RIGHT_PENALTY=0.048131
PRIMER_LEFT_SEQUENCE=GGAGTCACTTCTTTCCATCCAC
PRIMER_RIGHT_SEQUENCE=AAGCTCAAGCTAAATCTGCACC
PRIMER_LEFT=433,22
PRIMER_RIGHT=638,22
PRIMER_LEFT_TM=59.979
PRIMER_RIGHT_TM=60.048
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.0794
PRIMER_LEFT_1_PENALTY=0.020747
PRIMER_RIGHT_1_PENALTY=0.058624
PRIMER_LEFT_1_SEQUENCE=GGAGTCACTTCTTTCCATCCAC
PRIMER_RIGHT_1_SEQUENCE=AGACTCCAAGTCAGGCAGAGAC
PRIMER_LEFT_1=433,22
PRIMER_RIGHT_1=724,22
PRIMER_LEFT_1_TM=59.979
PRIMER_RIGHT_1_TM=60.059
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.1411
PRIMER_LEFT_2_PENALTY=0.110131
PRIMER_RIGHT_2_PENALTY=0.030986
PRIMER_LEFT_2_SEQUENCE=CAGGAGTCACTTCTTTCCATCC
PRIMER_RIGHT_2_SEQUENCE=CAGGCAGAGACACAGTTAGTGG
PRIMER_LEFT_2=431,22
PRIMER_RIGHT_2=713,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.969
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.1411
PRIMER_LEFT_3_PENALTY=0.110131
PRIMER_RIGHT_3_PENALTY=0.030986
PRIMER_LEFT_3_SEQUENCE=AGGAGTCACTTCTTTCCATCCA
PRIMER_RIGHT_3_SEQUENCE=CAGGCAGAGACACAGTTAGTGG
PRIMER_LEFT_3=432,22
PRIMER_RIGHT_3=713,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.969
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=0.1583
PRIMER_LEFT_4_PENALTY=0.110131
PRIMER_RIGHT_4_PENALTY=0.048131
PRIMER_LEFT_4_SEQUENCE=AGGAGTCACTTCTTTCCATCCA
PRIMER_RIGHT_4_SEQUENCE=AAGCTCAAGCTAAATCTGCACC
PRIMER_LEFT_4=432,22
PRIMER_RIGHT_4=638,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=60.048
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_01504 Ps_contig_mira-and-tgicl-ass_franssen_14541
SEQUENCE=GACACATAACATAACCAACTAACCGAAAAAGGGTGTCAATGTCCAAATGACCACATCACATTCACATCTAAGAAGAAGCCATTTCTGAAACTCGGTATCCTCTAATGGCCTCCAATCTCACCAACCTCACCATTCCTCCTCCTCCTCCTCCTTCTTCTTCTTACCCGTCCCCCAATCGACTCCCAACCCATCTCCAACAATCATCTACAGTACGGTCCCACTTCCTTCGTACGGTCCCCTCCCT
TARGET=134,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3969
PRIMER_LEFT_PENALTY=0.093130
PRIMER_RIGHT_PENALTY=0.303792
PRIMER_LEFT_SEQUENCE=CAACTAACCGAAAAAGGGTGTC
PRIMER_RIGHT_SEQUENCE=AGATGATTGTTGGAGATGGGTT
PRIMER_LEFT=15,22
PRIMER_RIGHT=205,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=59.696
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.3969
PRIMER_LEFT_1_PENALTY=0.093130
PRIMER_RIGHT_1_PENALTY=0.303792
PRIMER_LEFT_1_SEQUENCE=ACTAACCGAAAAAGGGTGTCAA
PRIMER_RIGHT_1_SEQUENCE=AGATGATTGTTGGAGATGGGTT
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=59.696
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=0.3969
PRIMER_LEFT_2_PENALTY=0.093130
PRIMER_RIGHT_2_PENALTY=0.303792
PRIMER_LEFT_2_SEQUENCE=AACTAACCGAAAAAGGGTGTCA
PRIMER_RIGHT_2_SEQUENCE=AGATGATTGTTGGAGATGGGTT
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=205,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=59.696
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.3994
PRIMER_LEFT_3_PENALTY=0.095574
PRIMER_RIGHT_3_PENALTY=0.303792
PRIMER_LEFT_3_SEQUENCE=CTCTAATGGCCTCCAATCTCAC
PRIMER_RIGHT_3_SEQUENCE=AGATGATTGTTGGAGATGGGTT
PRIMER_LEFT_3=99,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.696
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=0.4329
PRIMER_LEFT_4_PENALTY=0.129103
PRIMER_RIGHT_4_PENALTY=0.303792
PRIMER_LEFT_4_SEQUENCE=AAGCCATTTCTGAAACTCGGTA
PRIMER_RIGHT_4_SEQUENCE=AGATGATTGTTGGAGATGGGTT
PRIMER_LEFT_4=75,22
PRIMER_RIGHT_4=205,22
PRIMER_LEFT_4_TM=60.129
PRIMER_RIGHT_4_TM=59.696
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=131
=
PRIMER_SEQUENCE_ID=SSR_01505 Ps_contig_mira-and-tgicl-ass_franssen_14542
SEQUENCE=GTCGGAGGGAGGGGGGATGAGAGGGGAGGGACCTACGAAGGAGTGGGACCTACTGTAGATGATTTGTGGAGATGGGTTGGGATCGATTGGGGGACGGTAAGAAGAAGAAGGAGGAGGAGGAGGAGGAATGGTGAGGTTGGTGAGATTGGAGGCCATTAGAGGATACCGAGTTTCAGAAATGGCTT
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4329
PRIMER_LEFT_PENALTY=0.303792
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=AGATGATTTGTGGAGATGGGTT
PRIMER_RIGHT_SEQUENCE=AAGCCATTTCTGAAACTCGGTA
PRIMER_LEFT=56,22
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=59.696
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=129
PRIMER_PAIR_PENALTY_1=0.4684
PRIMER_LEFT_1_PENALTY=0.303792
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=AGATGATTTGTGGAGATGGGTT
PRIMER_RIGHT_1_SEQUENCE=CCATTTCTGAAACTCGGTATCC
PRIMER_LEFT_1=56,22
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=59.696
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.6010
PRIMER_LEFT_2_PENALTY=0.303792
PRIMER_RIGHT_2_PENALTY=0.297163
PRIMER_LEFT_2_SEQUENCE=AGATGATTTGTGGAGATGGGTT
PRIMER_RIGHT_2_SEQUENCE=ATCCTCTAATGGCCTCCAATCT
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=163,22
PRIMER_LEFT_2_TM=59.696
PRIMER_RIGHT_2_TM=60.297
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.6521
PRIMER_LEFT_3_PENALTY=0.303792
PRIMER_RIGHT_3_PENALTY=0.348289
PRIMER_LEFT_3_SEQUENCE=AGATGATTTGTGGAGATGGGTT
PRIMER_RIGHT_3_SEQUENCE=ACTCGGTATCCTCTAATGGCCT
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=170,22
PRIMER_LEFT_3_TM=59.696
PRIMER_RIGHT_3_TM=60.348
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=115
PRIMER_PAIR_PENALTY_4=0.8796
PRIMER_LEFT_4_PENALTY=0.303792
PRIMER_RIGHT_4_PENALTY=0.575816
PRIMER_LEFT_4_SEQUENCE=AGATGATTTGTGGAGATGGGTT
PRIMER_RIGHT_4_SEQUENCE=TATCCTCTAATGGCCTCCAATC
PRIMER_LEFT_4=56,22
PRIMER_RIGHT_4=164,22
PRIMER_LEFT_4_TM=59.696
PRIMER_RIGHT_4_TM=59.424
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_01506 Ps_contig_mira-and-tgicl-ass_franssen_14542
SEQUENCE=GTCGGAGGGAGGGGGGATGAGAGGGGAGGGACCTACGAAGGAGTGGGACCTACTGTAGATGATTTGTGGAGATGGGTTGGGATCGATTGGGGGACGGTAAGAAGAAGAAGGAGGAGGAGGAGGAGGAATGGTGAGGTTGGTGAGATTGGAGGCCATTAGAGGATACCGAGTTTCAGAAATGGCTT
TARGET=111,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4329
PRIMER_LEFT_PENALTY=0.303792
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=AGATGATTTGTGGAGATGGGTT
PRIMER_RIGHT_SEQUENCE=AAGCCATTTCTGAAACTCGGTA
PRIMER_LEFT=56,22
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=59.696
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=129
PRIMER_PAIR_PENALTY_1=0.4684
PRIMER_LEFT_1_PENALTY=0.303792
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=AGATGATTTGTGGAGATGGGTT
PRIMER_RIGHT_1_SEQUENCE=CCATTTCTGAAACTCGGTATCC
PRIMER_LEFT_1=56,22
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=59.696
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.6010
PRIMER_LEFT_2_PENALTY=0.303792
PRIMER_RIGHT_2_PENALTY=0.297163
PRIMER_LEFT_2_SEQUENCE=AGATGATTTGTGGAGATGGGTT
PRIMER_RIGHT_2_SEQUENCE=ATCCTCTAATGGCCTCCAATCT
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=163,22
PRIMER_LEFT_2_TM=59.696
PRIMER_RIGHT_2_TM=60.297
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.6521
PRIMER_LEFT_3_PENALTY=0.303792
PRIMER_RIGHT_3_PENALTY=0.348289
PRIMER_LEFT_3_SEQUENCE=AGATGATTTGTGGAGATGGGTT
PRIMER_RIGHT_3_SEQUENCE=ACTCGGTATCCTCTAATGGCCT
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=170,22
PRIMER_LEFT_3_TM=59.696
PRIMER_RIGHT_3_TM=60.348
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=115
PRIMER_PAIR_PENALTY_4=0.8796
PRIMER_LEFT_4_PENALTY=0.303792
PRIMER_RIGHT_4_PENALTY=0.575816
PRIMER_LEFT_4_SEQUENCE=AGATGATTTGTGGAGATGGGTT
PRIMER_RIGHT_4_SEQUENCE=TATCCTCTAATGGCCTCCAATC
PRIMER_LEFT_4=56,22
PRIMER_RIGHT_4=164,22
PRIMER_LEFT_4_TM=59.696
PRIMER_RIGHT_4_TM=59.424
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_01507 Ps_contig_mira-and-tgicl-ass_franssen_14548
SEQUENCE=ATATDCAAACAGATATTTGTCACTCATTTACTATAGTCGTTCACGTATTGACCTTGTGACAAGTTTATACCATAAACCAAGAAAGAAACAGATTGTAAATGTTCTCGTCCTCCTCAAAATCGATCTCATGTAAACTACATACAATGGGACACAGAATAAGTCTCCATGTTGAAAATCTCATAATTGTTCTAATATAATAGGATCGTTAAAACAAACTCAGATTAAGTACTAAAAGTCTCCATGTTGAAAATCTCCCAGCAATGTAGGTCAAACAATCATCAGAAGGAGTTGCTGTGGGAGGCTAGCTTACAGGATAGATGGTAGAAATTGCAACTTTAGTTCAAGCGTATGTCGACAAGATGTTTTTCTTTAGAGTGAACTCATCGGCCTTAGCAGGGCTGATAGACTTTTCAAGTATGCGCTCTAGACGCTGAATTTCTTTCTTAGCATAATCAGAACCTTTCTCCAGATAATTCTTGGCAGTTTTCAAGTAAATCTTCCCGTATCTAGAAGAAGAACCCTGAAGCTTCCCAACTTCCTCTTCTATTTGAGCAAATACGGTCTTCTTCTCTTCATCATTAGCAGCTACAAACTCCTTTACCAACACGTCCAAGTTCTCTACTATACCAGCCTCAGAAGTAAGCTGTCCTTTTGAATCTCGACTTGTTCCAGATTTCTCATTGATAAAAGCCACAAAGTCATCCAAGTCTCGTCCACCTCCATAATCTTCACCGGCTTTATTGCCCTTTGGGAAGAACTTCAAGGTAGGAAATCCACTCACGTCATACTTTTCAGCCAGGTCCCTATATTTGTCGGCATCCAAATTTGCAATCACTACATCGTCCTCCGATTTAAAGGCTGCAGCAACTTTTTCATAAATAGGAGCAAGACTTTTGCAATGTCCACACCATGGTGCATAAAACTCAACCAAGACATCTTTGGTTTCATCTAAGACAACTTCTTTGAAGCTTTCAGGTGTTAGCACCACTACACTAGATGGAGCTGTAACAATCTTTACATTAGTTCCTGCTTCTGTATTTATAAACTCGGCAAGTGAATCTGCAGTGCGTGGCCCTTCAAACTTTTTGGGTTCCAGAGATCCTTTTGGAAACCACTGAATCGTTGGGTACCCAGAGACTCCATATTTGGTGCACACACTCTTATGCTCATCACAGTCCACCTTGGCAATCAAAACAGATTTTGCTTTCTTGAAGGTGTTACCAAGCTTTTCATATTCTGGAGCAAGCTTTTTGCAGTGTCCACACCAAGGGGCGTAGAACTCAACGAGAGCTCCTTTGTCTTGACCAACTTCCTTCTCGAAATTCTCTTCAGAGAGCACAACGACGTCGTCTGCGGAGACCGATTGGGAGAGAAGCGCAAACGCTAACGCTGCGAAAGCTATACTTCTCCAGATCTGATACATCTCCATTTTCTTCTTCTTCTTCTTTCTCAGTTCTTTCTTTCTTTCGGTTTCTGCTTCAACTGTTACGGCACGTGACTCGTTATATAGGARGAAAAATACCCTCYSTTTCC
TARGET=1431,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01508 Ps_contig_mira-and-tgicl-ass_franssen_14548
SEQUENCE=ATATDCAAACAGATATTTGTCACTCATTTACTATAGTCGTTCACGTATTGACCTTGTGACAAGTTTATACCATAAACCAAGAAAGAAACAGATTGTAAATGTTCTCGTCCTCCTCAAAATCGATCTCATGTAAACTACATACAATGGGACACAGAATAAGTCTCCATGTTGAAAATCTCATAATTGTTCTAATATAATAGGATCGTTAAAACAAACTCAGATTAAGTACTAAAAGTCTCCATGTTGAAAATCTCCCAGCAATGTAGGTCAAACAATCATCAGAAGGAGTTGCTGTGGGAGGCTAGCTTACAGGATAGATGGTAGAAATTGCAACTTTAGTTCAAGCGTATGTCGACAAGATGTTTTTCTTTAGAGTGAACTCATCGGCCTTAGCAGGGCTGATAGACTTTTCAAGTATGCGCTCTAGACGCTGAATTTCTTTCTTAGCATAATCAGAACCTTTCTCCAGATAATTCTTGGCAGTTTTCAAGTAAATCTTCCCGTATCTAGAAGAAGAACCCTGAAGCTTCCCAACTTCCTCTTCTATTTGAGCAAATACGGTCTTCTTCTCTTCATCATTAGCAGCTACAAACTCCTTTACCAACACGTCCAAGTTCTCTACTATACCAGCCTCAGAAGTAAGCTGTCCTTTTGAATCTCGACTTGTTCCAGATTTCTCATTGATAAAAGCCACAAAGTCATCCAAGTCTCGTCCACCTCCATAATCTTCACCGGCTTTATTGCCCTTTGGGAAGAACTTCAAGGTAGGAAATCCACTCACGTCATACTTTTCAGCCAGGTCCCTATATTTGTCGGCATCCAAATTTGCAATCACTACATCGTCCTCCGATTTAAAGGCTGCAGCAACTTTTTCATAAATAGGAGCAAGACTTTTGCAATGTCCACACCATGGTGCATAAAACTCAACCAAGACATCTTTGGTTTCATCTAAGACAACTTCTTTGAAGCTTTCAGGTGTTAGCACCACTACACTAGATGGAGCTGTAACAATCTTTACATTAGTTCCTGCTTCTGTATTTATAAACTCGGCAAGTGAATCTGCAGTGCGTGGCCCTTCAAACTTTTTGGGTTCCAGAGATCCTTTTGGAAACCACTGAATCGTTGGGTACCCAGAGACTCCATATTTGGTGCACACACTCTTATGCTCATCACAGTCCACCTTGGCAATCAAAACAGATTTTGCTTTCTTGAAGGTGTTACCAAGCTTTTCATATTCTGGAGCAAGCTTTTTGCAGTGTCCACACCAAGGGGCGTAGAACTCAACGAGAGCTCCTTTGTCTTGACCAACTTCCTTCTCGAAATTCTCTTCAGAGAGCACAACGACGTCGTCTGCGGAGACCGATTGGGAGAGAAGCGCAAACGCTAACGCTGCGAAAGCTATACTTCTCCAGATCTGATACATCTCCATTTTCTTCTTCTTCTTCTTTCTCAGTTCTTTCTTTCTTTCGGTTTCTGCTTCAACTGTTACGGCACGTGACTCGTTATATAGGARGAAAAATACCCTCYSTTTCC
TARGET=1454,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01509 Ps_contig_mira-and-tgicl-ass_franssen_14552
SEQUENCE=GAACTGATTAGATTAATCCTGATTTGAAGGTCCTTTATGACATTAAGTGATTCCAACTTCTTTCTATTACAGTTGCTGATGATCGATCCGTAATCCTTCCGGAGTCAATTTCATGCTTTAGTTCTCTCCTGTACGACTGCGTTTTCAACACCAAAAACTCTCTCTCTCACAACCATGGTTTTGCTACGATTAGATCCGTTTCTCAATAAACTCACCAGCATGTTCGAGCGTAGCACTGACAAGGCTTCTGTTTGGGTTACTCTCAAACGATCATCCTTGAAATCTAAAGTTTGGAAGAATAAATTGGTTACTGCTGGTGAGACAATTGAGTATG
TARGET=158,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2429
PRIMER_LEFT_PENALTY=0.232784
PRIMER_RIGHT_PENALTY=0.010077
PRIMER_LEFT_SEQUENCE=GATGATCGATCCGTAATCCTTC
PRIMER_RIGHT_SEQUENCE=CGGATCTAATCGTAGCAAAACC
PRIMER_LEFT=77,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.767
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.3170
PRIMER_LEFT_1_PENALTY=0.306903
PRIMER_RIGHT_1_PENALTY=0.010077
PRIMER_LEFT_1_SEQUENCE=CTGATGATCGATCCGTAATCCT
PRIMER_RIGHT_1_SEQUENCE=CGGATCTAATCGTAGCAAAACC
PRIMER_LEFT_1=75,22
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=60.307
PRIMER_RIGHT_1_TM=59.990
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=123
PRIMER_PAIR_PENALTY_2=0.5376
PRIMER_LEFT_2_PENALTY=0.232784
PRIMER_RIGHT_2_PENALTY=0.304841
PRIMER_LEFT_2_SEQUENCE=GATGATCGATCCGTAATCCTTC
PRIMER_RIGHT_2_SEQUENCE=CTCGAACATGCTGGTGAGTTTA
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=59.767
PRIMER_RIGHT_2_TM=60.305
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=151
PRIMER_PAIR_PENALTY_3=0.5426
PRIMER_LEFT_3_PENALTY=0.232784
PRIMER_RIGHT_3_PENALTY=0.309780
PRIMER_LEFT_3_SEQUENCE=GATGATCGATCCGTAATCCTTC
PRIMER_RIGHT_3_SEQUENCE=AGTAACCCAAACAGAAGCCTTG
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=260,22
PRIMER_LEFT_3_TM=59.767
PRIMER_RIGHT_3_TM=59.690
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.5685
PRIMER_LEFT_4_PENALTY=0.558430
PRIMER_RIGHT_4_PENALTY=0.010077
PRIMER_LEFT_4_SEQUENCE=TAATCCTTCCGGAGTCAATTTC
PRIMER_RIGHT_4_SEQUENCE=CGGATCTAATCGTAGCAAAACC
PRIMER_LEFT_4=90,22
PRIMER_RIGHT_4=197,22
PRIMER_LEFT_4_TM=59.442
PRIMER_RIGHT_4_TM=59.990
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_01510 Ps_contig_mira-and-tgicl-ass_franssen_14561
SEQUENCE=AAGGGAAAAGTTTACCCAACTTAATTCCAGTTTAAAGGTACGTACCCCCAAAAACTACCCTTTAGTCATATTTTGTCTAAANAAATTTTTAAGTTAAGTANACCCTTATTTTATTCTGTTGTGTAACTAGAAGTCACCTATTTGTGTATATTATTATTATTATTGACTCTAGTGATTTTGTTATGTGTATCATGATGGACGCCGACTATGAGAACAATGTTAGTCTCATTACATTCTAAATTGCCATGTTTGAGGRCAGTAATCTTCC
TARGET=149,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01511 Ps_contig_mira-and-tgicl-ass_franssen_14562
SEQUENCE=CTTATATGGAAACTGATATGGATTCTTAGCATGTAATCAGAAGATTACTGTTCYCAAACATGGCAATTTAGAATGTAATGAGACTAACATTGTTCTACATAGTCGGCGTCCATCATGATACACATAACAAATCACTAGAGTCATAATAATAATAATATACACAAAGTAGGTGACTTTCTAGTTACACAANAGAAATAAAATAAGGGTNTCTTAYTAAAAATTNTTTAGA
TARGET=143,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01512 Ps_contig_mira-and-tgicl-ass_franssen_14574
SEQUENCE=GAACACCTAGGATTAATGAACTCTAGTATCTTAATCACGTGCAACASATGCTCATGAAGCAATTCAATGCAAAACAAACCGACCGCATCTCTCTCCTCAGCACAGTTCTCCCTCCGTCGTGCCAGAGGCCGTCCTCGACGACGGAGACGCCTCTAAAATCAAACCAAGAAAATTATCCAATCTGTTTTGATGCACTGATTCTAAAAATGCGAACGGATTATCCTTATTCTTTATGAGCTAAGTGAATCAAATAAAAAGCACGAAGAACCCTAACATGAAGAATCCAAATTAAACGCCAACGGAGGAGAATCAGGCCACCACTCGTTGGGGATTTGATTCCCCTCGTAAAACTCTACATTTTTGTAACAAGTTGAAGGAAAAAGGAGGGAGAAAAACTCACCTGCGATGTTCTCTGATGGTGGTGGTGGTGACAGCGACGATGAATGAAAATTTGATACCCATCTTGAAACTTCTTCTATGAATCTTCTTCTTCCTCCTCTCTTCGTCTTCTCTTTTCTTCTTCTCAATTCTTCTACGATTTGAGCAAACGAGAGTTTGGGGAACCAGAAGTAGAGAGTGTGTTGATGATGGAGCTTAGCACAAGCTGATTGAAGCTCTGATGACGAGCTTAAATGGCTTCCAAGGAGTGTGTATAGGTGAGGTTGAGAGAGGGAGAGTTGMKWTGGCAATGCTCATGATATACTTC
TARGET=417,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01513 Ps_contig_mira-and-tgicl-ass_franssen_14576
SEQUENCE=TTTAGAAGAWTMRRAGTAMACKTGGACATTAATATCCCTTCTAAGAAATAAAAGTCCTCCTTGCTGCTGGGTCCTAAATCTAAGAAAAATATGGCAATTCATAACAACATGTTCTTCTTTCTTTCTTCTCTCCTCTTCTTCACATTCCTCCAAATCCAAACCAAAGTCTTCTCTTTCCAATACAAAGTTGGAGATCTAAATGCTTGGAGCTTACCCTCCTCAACAAATCCACAAATCTACACCAAATGGTCCAAACTTCACAACTTCACCCTTGGAGACTCACTTTTGTTTCTATATCCACCAAGTCAAGATTCGTTGATTCAAGTATCAGAACAATCCTACAAAAGCTGCAACATTAAAGATCCAATATTGTTCATCAACAATGGTAACTCTTTGTTTAACATAACATCATCACATGGTGATTTTTACTTCACAAGTGGAGTAAATGGCCATTGCCAAAAGAATCAGAAGATTCATATATCTGTTGGAGGAATTGGAAATGTTGATGTAGAAGAAGCTAATAGTCCTAATTCATTGCCTGCTAATGGACCTTCCTATCAAGCAGCTTTTGGGAATATTCCAGTAGCTCCTTCTTCATCAAATTCACATCACATAACTTCAACTTTTCATGTATTCATCATTGGATTTGGATCTGTCATATATGCACTGTTATTTGGTTTAATGTAAAAGCTCAAGATGAAGATGATGTCATAAATGTTTATTTTTATTTTATATTTACTTTTCTTTACTTTGGTATTTCTTTTTGTCAATGATTTGAATATGTAATTCTTTATGTGGGATTAAATTTTTAAAGTTACTTTTGTTMTAWGATAATTGAAATATTCTTCCCCTCTTTGT
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01514 Ps_contig_mira-and-tgicl-ass_franssen_14582
SEQUENCE=TTTTTTYYTACWATAAAAAGTTTGCAATCATTTTTGTAAAACAAATATTAGAAGAAATATGTTCTTTTAAATGGATACATACATATAGACAAAATTTGGTTCTTCAATTAATTTGATCAAATAAATCAGATGATTGGCAGAGAACATGGTATCATTTTTGCTCAAGCTTTTTAAGTACTGTACAAAAGCCAAAGCCTCTGCAACTAAATTGGTTGCTAAGTAGATAGACTCTTATTCGAAGAAGGTAACGAAAGGAACATTCTTCCCTACTCAAAAGAAGAAGCTTAGTTGAGGCCAGAGGAGGAGTTTATGTCACTCCATGATGATGATGAGGTGCTGCACTTCACCTTCTTATGAAACTTGGGTGTTTTAGGTTGAGTAGGACACTTCGCTGACCTCCTTGGGATAAAGGGTCTGTCGAAATATGGCATAGGTTGGGCTTTTGGAACAAGTTCTTTCCGCAACCTTTTGATTTCTTCTTCTTCTTCCAACTTCTGCTGTCTCTCCCTTTCCAATTTATATTGTTCTATGAAGAGCAATTTTTCTGCCACTTGTTCATCAAACT
TARGET=320,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01515 Ps_contig_mira-and-tgicl-ass_franssen_14582
SEQUENCE=TTTTTTYYTACWATAAAAAGTTTGCAATCATTTTTGTAAAACAAATATTAGAAGAAATATGTTCTTTTAAATGGATACATACATATAGACAAAATTTGGTTCTTCAATTAATTTGATCAAATAAATCAGATGATTGGCAGAGAACATGGTATCATTTTTGCTCAAGCTTTTTAAGTACTGTACAAAAGCCAAAGCCTCTGCAACTAAATTGGTTGCTAAGTAGATAGACTCTTATTCGAAGAAGGTAACGAAAGGAACATTCTTCCCTACTCAAAAGAAGAAGCTTAGTTGAGGCCAGAGGAGGAGTTTATGTCACTCCATGATGATGATGAGGTGCTGCACTTCACCTTCTTATGAAACTTGGGTGTTTTAGGTTGAGTAGGACACTTCGCTGACCTCCTTGGGATAAAGGGTCTGTCGAAATATGGCATAGGTTGGGCTTTTGGAACAAGTTCTTTCCGCAACCTTTTGATTTCTTCTTCTTCTTCCAACTTCTGCTGTCTCTCCCTTTCCAATTTATATTGTTCTATGAAGAGCAATTTTTCTGCCACTTGTTCATCAAACT
TARGET=474,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01516 Ps_contig_mira-and-tgicl-ass_franssen_14625
SEQUENCE=CGGGATATTATAAAATTTTATATGATATAAATTCCCTTTCATGTTGGTCTTAAATTSATCAAACCTCAAACCCCTTCCTCATAATCTTCACACACATTCTCTCTCTCTCTCTTACTTCAAGTGAAACTGTGTTTGTCTTTATTTTCCTTTTGTTGTTTCTCATTGTAAACTTTCATTATTTCACACCTCTCTCTCTCACTCTCTCTCTGCTGAAGAAAATTGTTTGCAAGAACTGGTCTT
TARGET=98,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01517 Ps_contig_mira-and-tgicl-ass_franssen_14625
SEQUENCE=CGGGATATTATAAAATTTTATATGATATAAATTCCCTTTCATGTTGGTCTTAAATTSATCAAACCTCAAACCCCTTCCTCATAATCTTCACACACATTCTCTCTCTCTCTCTTACTTCAAGTGAAACTGTGTTTGTCTTTATTTTCCTTTTGTTGTTTCTCATTGTAAACTTTCATTATTTCACACCTCTCTCTCTCACTCTCTCTCTGCTGAAGAAAATTGTTTGCAAGAACTGGTCTT
TARGET=187,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01518 Ps_contig_mira-and-tgicl-ass_franssen_14625
SEQUENCE=CGGGATATTATAAAATTTTATATGATATAAATTCCCTTTCATGTTGGTCTTAAATTSATCAAACCTCAAACCCCTTCCTCATAATCTTCACACACATTCTCTCTCTCTCTCTTACTTCAAGTGAAACTGTGTTTGTCTTTATTTTCCTTTTGTTGTTTCTCATTGTAAACTTTCATTATTTCACACCTCTCTCTCTCACTCTCTCTCTGCTGAAGAAAATTGTTTGCAAGAACTGGTCTT
TARGET=199,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01519 Ps_contig_mira-and-tgicl-ass_franssen_14626
SEQUENCE=CGGGGATCAAACCTACCAAACCCCBTCCTSATAATCTTCACACACATTCTCTCTCTCTCTCTTACTTCAAGTGAAACTGTGTTTGTCTTATTTCCTTTTGTTGTTTCTCATTGTAAACTTTCATTATTTCACACCTCTCTCTCTCTCTCTCTCTGCTGAAGAAATTGTTTGCAAGAACGTGGTTCTTCTCTTCCCTTACCACAGTAACTTACCTACCAACTAAAGTTACGTTTACCAAACCTAACGTTAGAAGTAGTTA
TARGET=48,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01520 Ps_contig_mira-and-tgicl-ass_franssen_14626
SEQUENCE=CGGGGATCAAACCTACCAAACCCCBTCCTSATAATCTTCACACACATTCTCTCTCTCTCTCTTACTTCAAGTGAAACTGTGTTTGTCTTATTTCCTTTTGTTGTTTCTCATTGTAAACTTTCATTATTTCACACCTCTCTCTCTCTCTCTCTCTGCTGAAGAAATTGTTTGCAAGAACGTGGTTCTTCTCTTCCCTTACCACAGTAACTTACCTACCAACTAAAGTTACGTTTACCAAACCTAACGTTAGAAGTAGTTA
TARGET=135,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01521 Ps_contig_mira-and-tgicl-ass_franssen_14628
SEQUENCE=AGCAGCAAACTCTCTTGCATTKCAGACAAAGATTCTTATTATTATTGTARCAAGCGTCGTCAAAGGATAATACCCGAAATGATCATTGAAGCCATAATCTTATTGCTAGAACTTTGTACAAATGAATTGAGAGAATATAATAACAAAAAATTAAGCTTCTTACTTGCCTCTCCCAACACTAAAATAAACATAGATCAGTGCAACAAACTCATCTACTCGTTTATAGCAGCTCCAACTGGGACTATACATCATGAAAGAGTCTCCTTATCTGGTCAACTGTTGCAGGCATCATCGTTGCTGGGCAACGCTTATACTGAGTAACAAACTTGAATACACAGGCATAGCAGAAGACGAAACCTGAAACCGTAATTACAGATGGATTTACGCGCTTCTGCAAGCACAGGGGGCAAACTGTTCTGTCTGGCGGTAACTGTACCCCTTCTTTGGCTACCTTTGGTGGTGGAGGAGGAGGGGGAGGAGGATATACAGTTGGAGCCGACATTCTTTCCTCAGCAGATTGATACCACCATTCCATCATTTTAAAGAAGAAAACTGCTGCAATCAAACCGGTTTGGGCATAGTCAAGTACAGTGTATGTACAGCTGAGCAATGCTCCCTGTAATGTCTTTACCCATTGAGGACCACGGAGTCTCTCACGTTCACGGCTACGTATCTTAGAAATTCTTGAAGAGGCGTCCATCAGCTCTTGTCCACTGGCTCGGCACACGTGAATCCCAAGTGCGTGCAGCGCTAGTGAGTAGTAACCAGTAGCATCCAATAGGTATAACATTTGGTATGCGAACTGGAATCCCTCGGTACCAGCATGTAACAAGGGGTAACAAATTCCGACAATTTTCTTGAAATCTCTTTGTGATGCGTGTTGTAACGGAGGCACGAGCATCCAAGGTTGACGTGGACACCAGAGAATTCTGTTCAAAATCATATGATTCGTTTTCATCTCCCCAAAGACTGGCTTGAATCCTTGCTTCCCTTTCTTTATTGTAGATTGAATGCAGCTTCGATTTCAAATACGGCAACACAACCAAAAAGACAAGAGAAACAAGCTTTTGGCGTCTGCGTAACTGTGAAGTAGTAGTAGTAGCAGCAGCATCACTATCGTTATTGAGTTTGATATTTACCGGTCTTCTTCGGAGACCATAGAGAGATTCGGAAAAAGAAGCATCTGTTGTTCGTAAACTGTGAGCTTCGAGAACGAGCATGAGTAAGGAGAAAGATTCGTCTTCGTAATCTAGTAATTTATGAAGGAAAGGTGTTCGTAATGCTAAAACACCGATTGAGTATGTCAGAGCGGCGCGAAGACTTCGCGGAAGCTGATCGGGCCGGCCCGCCATTCCTACGRAAAACGTCGGCCGACTGCCTTGTCCACCTA
TARGET=1089,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01522 Ps_contig_mira-and-tgicl-ass_franssen_14629
SEQUENCE=GCTCAATCAAGTGGAGGATCGGATGCTAAAGAAGAAGATTGGATTGCGGAGAGGAGAAAAGAAGAAGAGAAGAAAATGGTTAGGGGGAGAAAAAAGGGTTTGAAACAACTTGTGAAGCAGTGTGTGATCAAGTCAAGAAGAGAACATGATAATGATAATGAGTGATTAAGAAGGTTGAATTTAGGAATTGTATGCGAGTTATATATTGTTTAGTTAAGGTAGTTGAATTAACAAAAGTGTCATGAATGTGGATTTTGGTTTGGTTTGGTTTTCATTTATACTATTATTTATGAGTAATTAAGTCGTGTATTTATTACTACTGTGGACTATTTCTGTGATATGTTGACACATTTGAATTCATTATGGTATA
TARGET=254,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01523 Ps_contig_mira-and-tgicl-ass_franssen_14640
SEQUENCE=CCTAACAAAAGTGCTCAGATAGAATTTTATTTTGAAAAAGATAGATGAGGGGAGAAACCTTGTACTTGGTTTCAAAAACAGAGGAGATTCTACCAAGTTTCACTTTAATTTAATTGTATAATTTACCACTAAAGTTTACAATGTCACTTTCAGATATAGTGGTATAAGAATTAGTAACTTTCATAAAACAAATGAATTAAGAATACTAGAAATTAACAGCAGCAGCAACAACAAAAACAAAAAGCTAAATTTTGCAAAGTGGCAAATTGGCTATACCAAAACAAATCCAAACAAAACAAAAGCAAAAGACAAGAATAGAGAGTTAAGTCCCAAATACTCCAAGACCATAGAAGCAGCAGAGCTTGGTGTTGAATGGTTGTGTTGAGATGAAGTAGGAGGAGGAGGAAGTGGATGAACAAGAACTGAAACTTTCATTCCATTTAAGCATTGGCCATTACCACAAATGAAGTAATATCTCATAGTCTTATTCAGTAATATGAAATCTTTCCCACTAGACCAATTTCCAACAGCACTGTCTAGGGTACAGTTATCATAACCAGTTTGGTTCACTTCAAACACGTTGTACTGATTCTTCTGATACCTAAATGAAATGAAATCGCCGACATAGAAAGTGTGGTTATTGGCCCAAAGAGTGTAGTTCATACCAGGGTTCCAGCCACGGTTAGCACCTACGATGTGGTCGGTTGCAGAAACAGGAAGAGGAGAGAGTGTGAAGAGAAAAACGAAGAGGAAGAAGAAGGTTAGTATGAAGAATGAGGAAGAAAGAGCCATGTTAGGTTCAGAAGAGAATGAGGTATCGTTCYTTTTGTTATATTACTTTCCCT
TARGET=394,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01524 Ps_contig_mira-and-tgicl-ass_franssen_14641
SEQUENCE=AACTCTCTATTCTTGTCTTTTGCTTTTCTTTTTGTTTTGGGTATTTGTTTTTGGTATAGCCAATTTGCCACTTTGCAAAATTTAGCTTTTTGTTTTTTGTTGTTGTTGCCTGCTGTTAATTTCTAGTATTCTTAATTCATTGTTTTATCAAAGTTACTAATTCTTATACCACTATATCTGAAAGTGACATTAAAGGAAAAAAAAA
TARGET=97,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01525 Ps_contig_mira-and-tgicl-ass_franssen_14645
SEQUENCE=ACGGGGGGAATCCTCTCTCTCTCTCTTCACCAAACCCCTCAACAACATGGGAACCCCACAGTCCAATCTTCCCCCAGGTTTCAGATTCCACCCTACTGACGCAGAACTCATTCTTCACTATCTTAGAAAAAAAAATTGCTTCTATTCCCTTACCTGTTTCCATCATTGCTGAAGTTGATATTTACAAATTAGATCCATGGGATTTACCAGCTAAGGCTTTGTTTGGTGAGAAAGAGTGGTACTTTTCAGTCCTAGAGATAGAAAGTACCCAAATGGTGCAAGGCCAAACAGGGCTGCTGCTTCAGGGTATTGGAAAGCCACTGGAACTGATAAGACGATAGTTGCTTCATTGCCATGTGGAGGAAGATCACAAGAGAATATTATTGGTGTCAAAAAGGCACTTGTTTTTTACAAAGGAAAACCTCCAAAGGGTATTAAAACTAATTGGATCATGCATGAATATCGTCTCGTCGATAATAATAAACCAATTAAACTCAAAGATTCTTCCATGAGGTTGGATGATTGGGTATTATGTCGAATTTACAAGAAATCAAAATGCGCGCTAACTTCAACCGAATCCGAGACAATGATAGGCGAAGTCAAGCACTTGGATGATGCGCAATACAAAGAAACTCATTTTCCGATCACAAAAAACACAACATCTTCTCATCAAAACACACTTATGTCACAAAAATCAGTGTCTTTCTA
TARGET=13,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01526 Ps_contig_mira-and-tgicl-ass_franssen_14658
SEQUENCE=AACTTAGTGATCAGATCCTTCACATGACTTGACTTTTCTCCCTTAGACGGATTCTTGGTGCGGCCAATATCGTGTCTATCATAACTTTCGAGAACTTTATGACTCGAAGGAGTACTTCTCTGTTCATATATATATCCGGAAGAGTGAAAACCGTTGTGGAGACCGAGCAAGAAGACGAGAGGGACGAGCATGGAGAGAATGACGAGACCGAGAACCGCAATTACGAAACCTCGCCACCGTCGTTTTGCGGGGACGCCGTAAGATGGAACCGCGCCCAAACCACCGCTCTTCATCGTTTTTTCTTACCCCCCGCGTACTCTG
TARGET=126,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3585
PRIMER_LEFT_PENALTY=0.243298
PRIMER_RIGHT_PENALTY=0.115189
PRIMER_LEFT_SEQUENCE=TTGACTTTTCTCCCTTAGACGG
PRIMER_RIGHT_SEQUENCE=GTTCTCGGTCTCGTCATTCTCT
PRIMER_LEFT=28,22
PRIMER_RIGHT=214,22
PRIMER_LEFT_TM=59.757
PRIMER_RIGHT_TM=59.885
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.3954
PRIMER_LEFT_1_PENALTY=0.280216
PRIMER_RIGHT_1_PENALTY=0.115189
PRIMER_LEFT_1_SEQUENCE=TTCTCCCTTAGACGGATTCTTG
PRIMER_RIGHT_1_SEQUENCE=GTTCTCGGTCTCGTCATTCTCT
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=214,22
PRIMER_LEFT_1_TM=59.720
PRIMER_RIGHT_1_TM=59.885
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.8030
PRIMER_LEFT_2_PENALTY=0.243298
PRIMER_RIGHT_2_PENALTY=0.559665
PRIMER_LEFT_2_SEQUENCE=TTGACTTTTCTCCCTTAGACGG
PRIMER_RIGHT_2_SEQUENCE=CTCCACAACGGTTTTCACTCTT
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=160,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=60.560
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=133
PRIMER_PAIR_PENALTY_3=0.8399
PRIMER_LEFT_3_PENALTY=0.280216
PRIMER_RIGHT_3_PENALTY=0.559665
PRIMER_LEFT_3_SEQUENCE=TTCTCCCTTAGACGGATTCTTG
PRIMER_RIGHT_3_SEQUENCE=CTCCACAACGGTTTTCACTCTT
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=160,22
PRIMER_LEFT_3_TM=59.720
PRIMER_RIGHT_3_TM=60.560
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.9452
PRIMER_LEFT_4_PENALTY=0.829995
PRIMER_RIGHT_4_PENALTY=0.115189
PRIMER_LEFT_4_SEQUENCE=CTCCCTTAGACGGATTCTTGGT
PRIMER_RIGHT_4_SEQUENCE=GTTCTCGGTCTCGTCATTCTCT
PRIMER_LEFT_4=37,22
PRIMER_RIGHT_4=214,22
PRIMER_LEFT_4_TM=60.830
PRIMER_RIGHT_4_TM=59.885
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_01527 Ps_contig_mira-and-tgicl-ass_franssen_14666
SEQUENCE=CATAGTAAGTGACCACCCAACTGAAACTACCACCGCNTGACAAAACAACTAGTGAAGTGTATACAGGTTGCGAATACTTCTGTCTGCATCAGGAGATAGATATTTGCCATTATCCCTATCAAGATCTAGTTGCTAAGGGGAACTCATAGCGGTCATTGATCTTAACCATAGTGTCTCGCACAAAATCATACTCGAACCGTTTTAACTGAAGCTGAAGAACCGGTGGAAAATCAATGAACAATACACCCTTCTTTGCTTCCTGTAAACCATACTGCTCTGCATGATACTTGTTATCACCCTCAAGTTTTTCCACTTCCACATACTTATCGAAAGAAGCATACACATCAGTACAGCCTTTGACATCCAGCTGAAGATCATAAAATGATTCTTTTCGTGTTGATTTATAATCCACATTGATGCATTCAATATAGTTCATATGGTGGCCTTCAAACAACTGCTGTATAGTGCCTTCAACAACAGTTCCCTTCATTTTATCTTCTAGTCTTTTCACAAAGAACCCTATTAAGTTCTTGGACATCATGCTGCATGAATGAATCATATGTATCCCATCCAAAAGATTTTGTTAACTCTTTTGTTGATACGCTGCTGTCATTATACTGCAACTTGTAAAACAAACTCTGTAATGCCAGAGGGATGCTCCCAGATGGCATATCATTTTCGGTTGTGGGCATATGGTACACAGCCTTTCTGAAATAAGGGATGTGATACAATGTCTGAAGAAGAAGAATTCATGTAACAAGTAGCTCCTTG
TARGET=737,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01528 Ps_contig_mira-and-tgicl-ass_franssen_14679
SEQUENCE=TTAGTAGAATAGAGATAGAACTCTAGAAGCAGTGGTGGCGGAGGCCGTTCGCCCACTTGATTTAGTTTGGTTACTTGGTTATAGATTTCTTCTCATGATGATTGATAAGATATATATAGTATGGTAGTGTAAATATATATATTACCATTTGACATTGTGTTAAGGGCAAGGTTATATGGTTGGATTGTGGATTTTTCATGGCTAAAAGACAGGATTAGAAAAAGTTT
TARGET=133,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7265
PRIMER_LEFT_PENALTY=0.444124
PRIMER_RIGHT_PENALTY=0.282356
PRIMER_LEFT_SEQUENCE=GAACTCTAGAAGCAGTGGTGGC
PRIMER_RIGHT_SEQUENCE=ACCTTGCCCTTAACACAATGTC
PRIMER_LEFT=17,22
PRIMER_RIGHT=171,22
PRIMER_LEFT_TM=60.444
PRIMER_RIGHT_TM=60.282
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.7382
PRIMER_LEFT_1_PENALTY=0.455824
PRIMER_RIGHT_1_PENALTY=0.282356
PRIMER_LEFT_1_SEQUENCE=GTTCGCCCACTTGATTTAGTTT
PRIMER_RIGHT_1_SEQUENCE=ACCTTGCCCTTAACACAATGTC
PRIMER_LEFT_1=46,22
PRIMER_RIGHT_1=171,22
PRIMER_LEFT_1_TM=59.544
PRIMER_RIGHT_1_TM=60.282
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.8669
PRIMER_LEFT_2_PENALTY=0.584504
PRIMER_RIGHT_2_PENALTY=0.282356
PRIMER_LEFT_2_SEQUENCE=GCCCACTTGATTTAGTTTGGTT
PRIMER_RIGHT_2_SEQUENCE=ACCTTGCCCTTAACACAATGTC
PRIMER_LEFT_2=50,22
PRIMER_RIGHT_2=171,22
PRIMER_LEFT_2_TM=59.415
PRIMER_RIGHT_2_TM=60.282
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=1.0604
PRIMER_LEFT_3_PENALTY=0.444124
PRIMER_RIGHT_3_PENALTY=0.616243
PRIMER_LEFT_3_SEQUENCE=GAACTCTAGAAGCAGTGGTGGC
PRIMER_RIGHT_3_SEQUENCE=GCCCTTAACACAATGTCAAATG
PRIMER_LEFT_3=17,22
PRIMER_RIGHT_3=166,22
PRIMER_LEFT_3_TM=60.444
PRIMER_RIGHT_3_TM=59.384
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=150
PRIMER_PAIR_PENALTY_4=1.2001
PRIMER_LEFT_4_PENALTY=0.444124
PRIMER_RIGHT_4_PENALTY=0.755994
PRIMER_LEFT_4_SEQUENCE=GAACTCTAGAAGCAGTGGTGGC
PRIMER_RIGHT_4_SEQUENCE=CCCTTAACACAATGTCAAATGG
PRIMER_LEFT_4=17,22
PRIMER_RIGHT_4=165,22
PRIMER_LEFT_4_TM=60.444
PRIMER_RIGHT_4_TM=59.244
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_01529 Ps_contig_mira-and-tgicl-ass_franssen_14680
SEQUENCE=ACTCTAGAAGCAGTGGTGGCGGAGGCCGTTCGCCCGCTTGATTTAGTTTGGTTATTTGGTTATAGATTTCTTCTCATTGATGAGATATATATAGTATGGTAGTGTAAATATATATATTACCATTTGACATTGTGTTAAGGGCAAGGTTATATGGTTGGATTGTGGATTTTTCATG
TARGET=108,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6300
PRIMER_LEFT_PENALTY=0.347655
PRIMER_RIGHT_PENALTY=0.282356
PRIMER_LEFT_SEQUENCE=CCCGCTTGATTTAGTTTGGTTA
PRIMER_RIGHT_SEQUENCE=ACCTTGCCCTTAACACAATGTC
PRIMER_LEFT=32,22
PRIMER_RIGHT=146,22
PRIMER_LEFT_TM=60.348
PRIMER_RIGHT_TM=60.282
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=1.2217
PRIMER_LEFT_1_PENALTY=0.347655
PRIMER_RIGHT_1_PENALTY=0.874074
PRIMER_LEFT_1_SEQUENCE=CCCGCTTGATTTAGTTTGGTTA
PRIMER_RIGHT_1_SEQUENCE=AATCCAACCATATAACCTTGCC
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=160,22
PRIMER_LEFT_1_TM=60.348
PRIMER_RIGHT_1_TM=59.126
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=1.2953
PRIMER_LEFT_2_PENALTY=0.347655
PRIMER_RIGHT_2_PENALTY=0.947643
PRIMER_LEFT_2_SEQUENCE=CCCGCTTGATTTAGTTTGGTTA
PRIMER_RIGHT_2_SEQUENCE=TAACCTTGCCCTTAACACAATG
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=148,22
PRIMER_LEFT_2_TM=60.348
PRIMER_RIGHT_2_TM=59.052
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=1.4295
PRIMER_LEFT_3_PENALTY=0.347655
PRIMER_RIGHT_3_PENALTY=1.081865
PRIMER_LEFT_3_SEQUENCE=CCCGCTTGATTTAGTTTGGTTA
PRIMER_RIGHT_3_SEQUENCE=CCAACCATATAACCTTGCCCT
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=157,21
PRIMER_LEFT_3_TM=60.348
PRIMER_RIGHT_3_TM=60.082
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=1.4663
PRIMER_LEFT_4_PENALTY=0.347655
PRIMER_RIGHT_4_PENALTY=1.118632
PRIMER_LEFT_4_SEQUENCE=CCCGCTTGATTTAGTTTGGTTA
PRIMER_RIGHT_4_SEQUENCE=ATCCAACCATATAACCTTGCCC
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=159,22
PRIMER_LEFT_4_TM=60.348
PRIMER_RIGHT_4_TM=61.119
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_01530 Ps_contig_mira-and-tgicl-ass_franssen_14698
SEQUENCE=CYTTTGTTTTGGAGAGGAGAAATGTTGCTATTGAGTACATAATACATGTCTGAAACGTGTATACTTCTAGTTTTTATYCAATTTGTTTATTTGAGTTTGTTGTTGTTGGAGATTTCATGTTGATAATATGACTAAAAACAATGTATGATTGTATTGAAGAATTTGATTGGTAAACTTTAAAACCCCT
TARGET=97,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01531 Ps_contig_mira-and-tgicl-ass_franssen_14699
SEQUENCE=GGTTTTTTTTTAGGRGKRAAAGTTCCAATCAAATTTCTTCAATACTAATCATACATTGTTTTAGCATATTATCAACATGAAATCTCCAACAACAACAAACTCAAATAAACAAATTGGAATAAAAACTAGAAGTATACACGTTTCAGACATGTATTATGTACTCAATAGCAACATTTCTCCTCTACCAAAAAC
TARGET=87,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01532 Ps_contig_mira-and-tgicl-ass_franssen_14708
SEQUENCE=GTTCGRGGACAAACTAAAACAACAAAGCCTGTGTGAGAGAAACACTAAATCAAACATGTCATCATCTTCATCAATGACATTGAAAGGTGACTACATGGTGAGACCGAAGGAACCAACATGGCATGGTTGTTTACCATTATTTGAATGGAGTCTAACAGGTATGATATCTCACACTCCAATTATTTACTTTTATCACCCTCCACCAAATTGGCTTATCTCCCCCACTAAAATTGCCACCACTTTGAAAGATTCATTAAGCAAAATTCTAGTCCAGTTTTATCCACTTGCTGGTCGTTTACAAAATAAAGGAAATGGTCGTTTTGATCTAGAATGCAATGCAATGGGTGTTCAGTTCATCGAAGCAGAAACCTCATCAACATTATCTGATCTCGGTGATTTTTCACCTTCTTCAAACAATTATTGCCATTACCTTTTCCCGCATATTGATTATACGCTCCCAATGCAAGAGCTTCCTTTGCTTATTGTTCAGCTCACGAAGTTCAAATGTGGTGGCATTAGCATTAGTTTATTAATCTCTCACATAGTTGCCGATGGACAAAGCGTGACGCATTTCATTTCTGAATGGGCTCAAGTTGCACGTGGAGAGCCTTTGAAAAGGATTCCATATCTAGATAGAAACATATTGCGTGGTGGAGAACCATGCAACAATATTAATGACTGGGAATTTGAACAACTTCCACGTTTGTTGAAAAATTTGGATAATAGTTTTGAAGAAAGGAAGGAAAAAACAACCAATGCTATAATCAAAGTGAGTAAAAACCAACTTGAAAAGTTGAGAAAAACAGCGAATGAGAGCTGGAATAAACCTAGTAATGATCGAGGTTATTCAATATATGAAACAATAACCGGTCATATTTGGAGAAGTGCGTGTAAAGCTAGAGGCCATGAAAATAACCAACCAACTGCGATTGGCGTCTGCGTTGATTGGAGGAGTCGCGTTAAACCTAGTTTACCAAAAGAGTCTTTTGGGAATGCGATATTGGATGTGTTGGCTACTAGTCTCGCAGGTGATTTGATGTCTAAGCCATTAGGGTATGCTTCGAGTAGGATAAGAGAAGCAATAGAGAAGGTGAATGATGAGTATGTGAGGTCGGGGGTTGAGTATTTTAAAAAGCAATATGATTTAACTAAATTTCAAGATATTCATTGTATACAGAGTGACGACCACATGCCTTTTTATCGGAATCCTAATTTAATAGTCGTTAGTTGGTTGAAATTGCCTATTTATGGAATTGATTTTGGATGGGGAAAAGAGATTTATATGGGGCCAGGATTTCATGAAGAACTTGAAGGAGATTCGTTGATTCTTCCTAGTTCTCATGATGATGGATCTTTGTTGATTTTTATGTGTCTGCAAGAGGTCCATATGGATGCATTTAAGAGACATTTTTATGAAGGTGCTGAGTTTGAATTCCTTAAGGGAAGATTGTAAAAGGAGGTAGGTTTTTATCGATGTTTGTTTAATTAATTAAAAAAAATTAAAACAAACTATAAACTTAAAGGAGGTAGGTTTAATTATTTTATGTCAATGATGTTATGTTTTGTTATTAGTGATTTTGTTAATCTATTAAGTATGTGTCAAAGAGTTGCAGTTTGTATTTTTCAATTTATAAAGAGCAATGTTATTTTGACATAAAGTATTACACTTACCCAATAATGGACTATTTACGAGAA
TARGET=1482,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01533 Ps_contig_mira-and-tgicl-ass_franssen_14735
SEQUENCE=AAGAGTGGTATCAACGCAGAGTCYAAAAAATTTTAATTGAGTTGAGTGTGCTTTTTCTCTTCTGGCCTCCCTCCCTCCTAGAGAAAATCAATAGCTGTTGATGTGGGGGAAATATGTGACTATGGTTCTTTTCTTACACCGACTACCTTCAAAGTAAAGGTATTTTGAAAGGAAAGGAAAGATAAGCCTCTCTTGATGAGGTGATACACTGTTCTCATTTTGTGCATATATCCTGTAAAGAAATATTATTCAACCCTATTGGATTATGMKTAAA
TARGET=66,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01534 Ps_contig_mira-and-tgicl-ass_franssen_14735
SEQUENCE=AAGAGTGGTATCAACGCAGAGTCYAAAAAATTTTAATTGAGTTGAGTGTGCTTTTTCTCTTCTGGCCTCCCTCCCTCCTAGAGAAAATCAATAGCTGTTGATGTGGGGGAAATATGTGACTATGGTTCTTTTCTTACACCGACTACCTTCAAAGTAAAGGTATTTTGAAAGGAAAGGAAAGATAAGCCTCTCTTGATGAGGTGATACACTGTTCTCATTTTGTGCATATATCCTGTAAAGAAATATTATTCAACCCTATTGGATTATGMKTAAA
TARGET=167,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01535 Ps_contig_mira-and-tgicl-ass_franssen_14736
SEQUENCE=CTTTGCTGAGCGCAGAGTCAAAAAATTTATGAGTTGAGGGTGCTTTTTCTCTTCTGACCTCCCTCCCTCCAAGAGAAAATCAATAGCTGTTGATGTGGGGGAAATATGTGACTATGGTTCTTTTCTTACACCGACTACCTTCAAAGTAAAAGTATTTTGAAAGGAAAGGAAAGATAAACCTCTCTTGATGAGGTGATACACTGTTCTCATTTTGTGCATATATCCTGTAAGAAATAAATATTATTCAACCCTATTGGATTATGCTAATAATTTTGCACCTGCGTATATAAGATTGGCCTAATCGCCCCTGTACATTTTAAAGTTGAC
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1038
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.101479
PRIMER_LEFT_SEQUENCE=GAGGGTGCTTTTTCTCTTCTGA
PRIMER_RIGHT_SEQUENCE=ACTTTAAAATGTACAGGGGCGA
PRIMER_LEFT=35,22
PRIMER_RIGHT=322,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.899
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.4778
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.475486
PRIMER_LEFT_1_SEQUENCE=GAGGGTGCTTTTTCTCTTCTGA
PRIMER_RIGHT_1_SEQUENCE=AGGCCAATCTTATATACGCAGG
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=298,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=59.525
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.5493
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.547024
PRIMER_LEFT_2_SEQUENCE=GAGGGTGCTTTTTCTCTTCTGA
PRIMER_RIGHT_2_SEQUENCE=AACTTTAAAATGTACAGGGGCG
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=323,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=59.453
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.5801
PRIMER_LEFT_3_PENALTY=0.478666
PRIMER_RIGHT_3_PENALTY=0.101479
PRIMER_LEFT_3_SEQUENCE=TTGAGGGTGCTTTTTCTCTTCT
PRIMER_RIGHT_3_SEQUENCE=ACTTTAAAATGTACAGGGGCGA
PRIMER_LEFT_3=33,22
PRIMER_RIGHT_3=322,22
PRIMER_LEFT_3_TM=59.521
PRIMER_RIGHT_3_TM=59.899
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.5867
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.584386
PRIMER_LEFT_4_SEQUENCE=GAGGGTGCTTTTTCTCTTCTGA
PRIMER_RIGHT_4_SEQUENCE=CTTTAAAATGTACAGGGGCGAT
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=321,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=59.416
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_01536 Ps_contig_mira-and-tgicl-ass_franssen_14736
SEQUENCE=CTTTGCTGAGCGCAGAGTCAAAAAATTTATGAGTTGAGGGTGCTTTTTCTCTTCTGACCTCCCTCCCTCCAAGAGAAAATCAATAGCTGTTGATGTGGGGGAAATATGTGACTATGGTTCTTTTCTTACACCGACTACCTTCAAAGTAAAAGTATTTTGAAAGGAAAGGAAAGATAAACCTCTCTTGATGAGGTGATACACTGTTCTCATTTTGTGCATATATCCTGTAAGAAATAAATATTATTCAACCCTATTGGATTATGCTAATAATTTTGCACCTGCGTATATAAGATTGGCCTAATCGCCCCTGTACATTTTAAAGTTGAC
TARGET=159,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1038
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.101479
PRIMER_LEFT_SEQUENCE=TGCTTTTTCTCTTCTGACCTCC
PRIMER_RIGHT_SEQUENCE=ACTTTAAAATGTACAGGGGCGA
PRIMER_LEFT=40,22
PRIMER_RIGHT=322,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.899
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.1038
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.101479
PRIMER_LEFT_1_SEQUENCE=GAGGGTGCTTTTTCTCTTCTGA
PRIMER_RIGHT_1_SEQUENCE=ACTTTAAAATGTACAGGGGCGA
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=322,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=59.899
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.1548
PRIMER_LEFT_2_PENALTY=0.053348
PRIMER_RIGHT_2_PENALTY=0.101479
PRIMER_LEFT_2_SEQUENCE=GATGTGGGGGAAATATGTGACT
PRIMER_RIGHT_2_SEQUENCE=ACTTTAAAATGTACAGGGGCGA
PRIMER_LEFT_2=91,22
PRIMER_RIGHT_2=322,22
PRIMER_LEFT_2_TM=59.947
PRIMER_RIGHT_2_TM=59.899
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.4778
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.475486
PRIMER_LEFT_3_SEQUENCE=TGCTTTTTCTCTTCTGACCTCC
PRIMER_RIGHT_3_SEQUENCE=AGGCCAATCTTATATACGCAGG
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=298,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=59.525
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.4778
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.475486
PRIMER_LEFT_4_SEQUENCE=GAGGGTGCTTTTTCTCTTCTGA
PRIMER_RIGHT_4_SEQUENCE=AGGCCAATCTTATATACGCAGG
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=298,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=59.525
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_01537 Ps_contig_mira-and-tgicl-ass_franssen_14743
SEQUENCE=ATTTCCAGATGAAGACCGTTCATCCTCTTGAGACTCAAAACTATAAGATTCCGTCCCAGCTAGGGGGTTCTCAAATGATTCATTTCTTTGCATGCCAGGAGATGAGTATGGTGGAGGTTCCTCTCTTATCGGAGAACCAAGACCCTCATAAATAGAGGGACCACTACTTCGGCCAGCCTTGTCCGTAGTTTGTGATGCTCCACGTGAATTCGGCTTTGAACTGAACAGTGAAGGGTAGCTCATTCCACCAAAATGGGATGATATTGATGTTTCAACTGATCCAGTTGAATCTGGAGATGATGATCCCAATGACTTTGCATCGTCCTCCTCCAGTTCTGTAGTTTCTAGAAGTGTTTTTGCCCACATATCATCATAGCTAACAGAAGGCCTAGAGATTACATTCTCTTCATCAACTTCTGGGGCATCAGTTCCCGCACCCGCCAGAAATTCGTTTACTCTATTCATTGCTGGTGCATTTACTTTCCCCACAAGCCATCATCCCCGAGGTTTCGCTGCCCAAGCATTTACAAGGTCATCATCAAGGGCATCAGGTTCAGCAGCAGCAGGTGATTTGGATGATTCATAGATGAGGTCAGATATACCGGTGGCAACAGCTGGATCACTCAAACCAGTTGATGCACTAATATTGTGGCGTGTAACGATAGATATCAATGAGCTTG
TARGET=555,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1521
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.148509
PRIMER_LEFT_SEQUENCE=CCAAGCATTTACAAGGTCATCA
PRIMER_RIGHT_SEQUENCE=TCAACTGGTTTGAGTGATCCAG
PRIMER_LEFT=516,22
PRIMER_RIGHT=635,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.149
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=0.2732
PRIMER_LEFT_1_PENALTY=0.177596
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=TTTGCCCACATATCATCATAGC
PRIMER_RIGHT_1_SEQUENCE=CCACCGGTATATCTGACCTCAT
PRIMER_LEFT_1=355,22
PRIMER_RIGHT_1=607,22
PRIMER_LEFT_1_TM=59.822
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.3108
PRIMER_LEFT_2_PENALTY=0.215215
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=TCTAGAAGTGTTTTTGCCCACA
PRIMER_RIGHT_2_SEQUENCE=CCACCGGTATATCTGACCTCAT
PRIMER_LEFT_2=343,22
PRIMER_RIGHT_2=607,22
PRIMER_LEFT_2_TM=59.785
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.3261
PRIMER_LEFT_3_PENALTY=0.177596
PRIMER_RIGHT_3_PENALTY=0.148509
PRIMER_LEFT_3_SEQUENCE=TTTGCCCACATATCATCATAGC
PRIMER_RIGHT_3_SEQUENCE=TCAACTGGTTTGAGTGATCCAG
PRIMER_LEFT_3=355,22
PRIMER_RIGHT_3=635,22
PRIMER_LEFT_3_TM=59.822
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.3637
PRIMER_LEFT_4_PENALTY=0.215215
PRIMER_RIGHT_4_PENALTY=0.148509
PRIMER_LEFT_4_SEQUENCE=TCTAGAAGTGTTTTTGCCCACA
PRIMER_RIGHT_4_SEQUENCE=TCAACTGGTTTGAGTGATCCAG
PRIMER_LEFT_4=343,22
PRIMER_RIGHT_4=635,22
PRIMER_LEFT_4_TM=59.785
PRIMER_RIGHT_4_TM=60.149
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_01538 Ps_contig_mira-and-tgicl-ass_franssen_14749
SEQUENCE=CGTTACAACCAAATTAAACCCTTAACCTCAGATAAGAGAATAAACAATAAAGTAGAGACAAGGACATGCACAATAACAAACAAAATTACAAGAGTAGTAGTATAGTTATATATTATAGATATATATAACTAGACACTATTACATAAGTAAGCTCCTAATTATAGCTGCTTGAGCACTGTTGGAATCTGTAGTTGCCAAAAGTTGTGACAATCTTTCACTTAAATCATCAACTTCTCTATGCAAGTTTTTTATGTAGTTGCAAGTCTCTTGCAATACCTTAGAAGCTGAAACCTTGTTGGAGCGGGTAGCGCGAAGTTCGGGGATGAGTTGTTGTAACTTGGAGACGAGATCAGTGATTTGAGCATCAGTGATCTCAGAGGAAGCACCGGATTGTCTAGAACGAGATCTTCTGCTAGACATTATTCTATTAAGGTTGTAGTAACCAGTAACTAGTAAGTGTGTGAGTGTGTCAGAAACACTTATACTTGATGATGTAAATTGGTATAGGAAGCTTATAAAAATTTGTAGTTGAAGAAAAATAAATAGAGAGAATGAGATAGGTTGGTTGGTTTGTTGGTTGGTTGTCAGAGAAGAAAAGAGAGAAGAAAAGTGGTTATATGATTATTTTGTAGTGTTTGTTGATATGAAGGAGAGAAGAGAAGTGAGGTAGTGGGTTGAATTGAAGAGGACAAGTGCATCT
TARGET=560,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4060
PRIMER_LEFT_PENALTY=0.157217
PRIMER_RIGHT_PENALTY=0.248781
PRIMER_LEFT_SEQUENCE=CGAGATCAGTGATTTGAGCATC
PRIMER_RIGHT_SEQUENCE=TTCTTCTCTGACAACCAACCAA
PRIMER_LEFT=344,22
PRIMER_RIGHT=594,22
PRIMER_LEFT_TM=59.843
PRIMER_RIGHT_TM=59.751
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.4060
PRIMER_LEFT_1_PENALTY=0.157217
PRIMER_RIGHT_1_PENALTY=0.248781
PRIMER_LEFT_1_SEQUENCE=CGAGATCAGTGATTTGAGCATC
PRIMER_RIGHT_1_SEQUENCE=TTTCTTCTCTGACAACCAACCA
PRIMER_LEFT_1=344,22
PRIMER_RIGHT_1=595,22
PRIMER_LEFT_1_TM=59.843
PRIMER_RIGHT_1_TM=59.751
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.5235
PRIMER_LEFT_2_PENALTY=0.274768
PRIMER_RIGHT_2_PENALTY=0.248781
PRIMER_LEFT_2_SEQUENCE=CGGGGATGAGTTGTTGTAACTT
PRIMER_RIGHT_2_SEQUENCE=TTCTTCTCTGACAACCAACCAA
PRIMER_LEFT_2=317,22
PRIMER_RIGHT_2=594,22
PRIMER_LEFT_2_TM=60.275
PRIMER_RIGHT_2_TM=59.751
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.5235
PRIMER_LEFT_3_PENALTY=0.274768
PRIMER_RIGHT_3_PENALTY=0.248781
PRIMER_LEFT_3_SEQUENCE=CGGGGATGAGTTGTTGTAACTT
PRIMER_RIGHT_3_SEQUENCE=TTTCTTCTCTGACAACCAACCA
PRIMER_LEFT_3=317,22
PRIMER_RIGHT_3=595,22
PRIMER_LEFT_3_TM=60.275
PRIMER_RIGHT_3_TM=59.751
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.6451
PRIMER_LEFT_4_PENALTY=0.396351
PRIMER_RIGHT_4_PENALTY=0.248781
PRIMER_LEFT_4_SEQUENCE=TCAGTGATCTCAGAGGAAGCAC
PRIMER_RIGHT_4_SEQUENCE=TTTCTTCTCTGACAACCAACCA
PRIMER_LEFT_4=364,22
PRIMER_RIGHT_4=595,22
PRIMER_LEFT_4_TM=59.604
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_01539 Ps_contig_mira-and-tgicl-ass_franssen_14749
SEQUENCE=CGTTACAACCAAATTAAACCCTTAACCTCAGATAAGAGAATAAACAATAAAGTAGAGACAAGGACATGCACAATAACAAACAAAATTACAAGAGTAGTAGTATAGTTATATATTATAGATATATATAACTAGACACTATTACATAAGTAAGCTCCTAATTATAGCTGCTTGAGCACTGTTGGAATCTGTAGTTGCCAAAAGTTGTGACAATCTTTCACTTAAATCATCAACTTCTCTATGCAAGTTTTTTATGTAGTTGCAAGTCTCTTGCAATACCTTAGAAGCTGAAACCTTGTTGGAGCGGGTAGCGCGAAGTTCGGGGATGAGTTGTTGTAACTTGGAGACGAGATCAGTGATTTGAGCATCAGTGATCTCAGAGGAAGCACCGGATTGTCTAGAACGAGATCTTCTGCTAGACATTATTCTATTAAGGTTGTAGTAACCAGTAACTAGTAAGTGTGTGAGTGTGTCAGAAACACTTATACTTGATGATGTAAATTGGTATAGGAAGCTTATAAAAATTTGTAGTTGAAGAAAAATAAATAGAGAGAATGAGATAGGTTGGTTGGTTTGTTGGTTGGTTGTCAGAGAAGAAAAGAGAGAAGAAAAGTGGTTATATGATTATTTTGTAGTGTTTGTTGATATGAAGGAGAGAAGAGAAGTGAGGTAGTGGGTTGAATTGAAGAGGACAAGTGCATCT
TARGET=573,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4017
PRIMER_LEFT_PENALTY=0.872565
PRIMER_RIGHT_PENALTY=0.529160
PRIMER_LEFT_SEQUENCE=ACCGGATTGTCTAGAACGAGAT
PRIMER_RIGHT_SEQUENCE=TTCAATTCAACCCACTACCTCA
PRIMER_LEFT=384,22
PRIMER_RIGHT=683,22
PRIMER_LEFT_TM=59.127
PRIMER_RIGHT_TM=59.471
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=1.9142
PRIMER_LEFT_1_PENALTY=0.872565
PRIMER_RIGHT_1_PENALTY=1.041614
PRIMER_LEFT_1_SEQUENCE=ACCGGATTGTCTAGAACGAGAT
PRIMER_RIGHT_1_SEQUENCE=TCAATTCAACCCACTACCTCAC
PRIMER_LEFT_1=384,22
PRIMER_RIGHT_1=682,22
PRIMER_LEFT_1_TM=59.127
PRIMER_RIGHT_1_TM=58.958
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=299
PRIMER_PAIR_PENALTY_2=1.9581
PRIMER_LEFT_2_PENALTY=0.872565
PRIMER_RIGHT_2_PENALTY=1.085505
PRIMER_LEFT_2_SEQUENCE=ACCGGATTGTCTAGAACGAGAT
PRIMER_RIGHT_2_SEQUENCE=TCAATTCAACCCACTACCTCACT
PRIMER_LEFT_2=384,22
PRIMER_RIGHT_2=682,23
PRIMER_LEFT_2_TM=59.127
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=2.1410
PRIMER_LEFT_3_PENALTY=0.872565
PRIMER_RIGHT_3_PENALTY=1.268402
PRIMER_LEFT_3_SEQUENCE=ACCGGATTGTCTAGAACGAGAT
PRIMER_RIGHT_3_SEQUENCE=TTCAATTCAACCCACTACCTCAC
PRIMER_LEFT_3=384,22
PRIMER_RIGHT_3=683,23
PRIMER_LEFT_3_TM=59.127
PRIMER_RIGHT_3_TM=60.268
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=2.2041
PRIMER_LEFT_4_PENALTY=0.872565
PRIMER_RIGHT_4_PENALTY=1.331503
PRIMER_LEFT_4_SEQUENCE=ACCGGATTGTCTAGAACGAGAT
PRIMER_RIGHT_4_SEQUENCE=TTCAACCCACTACCTCACTTCTC
PRIMER_LEFT_4=384,22
PRIMER_RIGHT_4=678,23
PRIMER_LEFT_4_TM=59.127
PRIMER_RIGHT_4_TM=59.668
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_01540 Ps_contig_mira-and-tgicl-ass_franssen_14759
SEQUENCE=CCCAAAGTWATTAACAAATAACTAAAACATACACCACTCTATTCAGATTCCACTTTTTACCGAGTGTTAATTACATAATGAATTAATAATGTCTATTAGTACAATATAAACAAACATTAAATTAAATTAAATCTATCTTATCAACTTGGCTCATGTACACATATAAGATAACGGGCCATGGATGAATCATCGCCGCATTATTTAACATTTACATTAGACCCGACCCGACCCGACCCATAAGTGAAACTTCAGTTAGATGCAAATAATCGAGTGATACTCAGAACACCGTGGACGTTAGTTTTTCTCACTATTAAGGCTATCCCAAAGTAACGCGCACTCTTTCTTTGCGAAACCCACGCGCTCTTTCTCCAAATCATACACTACCTCAAATCCTTGCTGTTGATAATTCCCTAGTGTAGCTCCAGGTCCACCATCTAACTCAGTCTCATCACCACCGTTCATCAGCATCATACATCCAACTTTCCCTTTCCTTGTCTTCTCATCACCACCGTCCAAAAATTCATAAAAATAATTCTTCCTAGGCAACACCACACCCGAACTATTCCCAACAAAATGCAACGTCATCGTTGGTATCTGTGAAAATCCATTTAAATAATAACACGGAGCAAGACCCGTTTTCGTTTCTATCTCACTCGCTCGCTTGTGAACTCGTCCAACTCGTTTATCGAACTCAGCAACCACCGCTAGTGTTTCCACTCCTGAAACCACCGGATGCATCGCCGGAGACGCTTCCA
TARGET=116,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01541 Ps_contig_mira-and-tgicl-ass_franssen_14759
SEQUENCE=CCCAAAGTWATTAACAAATAACTAAAACATACACCACTCTATTCAGATTCCACTTTTTACCGAGTGTTAATTACATAATGAATTAATAATGTCTATTAGTACAATATAAACAAACATTAAATTAAATTAAATCTATCTTATCAACTTGGCTCATGTACACATATAAGATAACGGGCCATGGATGAATCATCGCCGCATTATTTAACATTTACATTAGACCCGACCCGACCCGACCCATAAGTGAAACTTCAGTTAGATGCAAATAATCGAGTGATACTCAGAACACCGTGGACGTTAGTTTTTCTCACTATTAAGGCTATCCCAAAGTAACGCGCACTCTTTCTTTGCGAAACCCACGCGCTCTTTCTCCAAATCATACACTACCTCAAATCCTTGCTGTTGATAATTCCCTAGTGTAGCTCCAGGTCCACCATCTAACTCAGTCTCATCACCACCGTTCATCAGCATCATACATCCAACTTTCCCTTTCCTTGTCTTCTCATCACCACCGTCCAAAAATTCATAAAAATAATTCTTCCTAGGCAACACCACACCCGAACTATTCCCAACAAAATGCAACGTCATCGTTGGTATCTGTGAAAATCCATTTAAATAATAACACGGAGCAAGACCCGTTTTCGTTTCTATCTCACTCGCTCGCTTGTGAACTCGTCCAACTCGTTTATCGAACTCAGCAACCACCGCTAGTGTTTCCACTCCTGAAACCACCGGATGCATCGCCGGAGACGCTTCCA
TARGET=217,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01542 Ps_contig_mira-and-tgicl-ass_franssen_14763
SEQUENCE=CTCCAAACTTACAGGTCATCCTCAAAATTAATATCAAAATACAAAATTCCGCAGAAGTTCGGGAATCGGAAAACCACCATCATCTGCACGCATTGATCCCATTTCAACATCAAGTTTCTCCTTTGGTGTCAAAATTTCGTCCACAATATTCGGAGTCAATGAACCAGTTGTCATATGTTCATCTTGCTGTACAAATGATCGGAAATTATTCTTTGAAGCGGGAATACTGGAACAGAGTGTTTCTCCAATATTTCTCACTGATCCCCTGTTGTATGGATTCCCCTTTTTATCATATTGGTACCGGAAGTTTTCATAAGTTGTCTGATTTGTACAAATGAGATAGAAATGGAAAGCTGTGAGGCCCCCGACAAACCAGACAGCAATGAAGCAATATATAATGAGAAAATCCGATACATAATCATGTGAGATTGCGTCCCAAACATTGTTCCTGGTCTTGGAAAGATTGACCCAAGAGAACACAAAGACATATAGGCACAAAAAGGTTGATGTTGTAATAAACATGAAGAAGTGCCGATAGTTTCGTCTTCCGATGCACTGGCCAACCCAGGGACAATGATGATCAAATCTCTGGACACAGTTGTTGCATATAGAACAATGAGAAGTACGAGGGGGTCGATAAAGCAAGCATGTATCACAAAACTTCACTTTCACCGTGTAACCATTAACCATTACATCTTTAGTTCGAGGTAGTTTCAAATGAGGGGTTGAACCATTAATCCACTCCATGGAAGGAGTAGGTATATTAAATGTTTCGTCGCATTCTGGAGGTGTTGAGTTTCTAGGAATAATTCCAGGATCTCTCGCGGAAGTCAAGAGGAGGAATAGGAAATCCAGAATAGTGAGAGTTAGACCGACAATCACTACGGGACACCAAAAAACATGACCACTACTTGCATTCTCATTCTTGGCTTTAAGATACATCTTGACACAAAATGTAATCGCAGGTGCAGCAATTAGGAACGATGTAAGGAACAAGGAAGCTACATCAGGACCAAAAACCAGTCTCCCACCACAGAGAAACTTATTGCCTCCTCTCCAAACTTGATAGAGCCTCCTCGGTTTCATATCATGGGCCCCAACTCCAGAAGGAGGAGACCCAAATTGCTGACTTCCATTCCTAATCATTGCAGCAGCACCACACGTGTGTCACCGAAATTGGTTTCTAACCTTGACAAAACCCATAAGAACCCAAATCAAGCAAGCTTCAACCCCGATTCAGAATCTTGTTAAAAATCAAAAATTTCGATCAGGGGTTCCTACAATTATCCTATTTCTGCAGTGAGAATGGAGAATGCAGATGAGAGAGAGAGAGAGAGAAAGAGAAGTTTGTTGTTTGGAATCACAGCAGAGGAATTGAGAATGAAGCTGTGACACCAAATTACAAACAGGAAAATTTGATCGAATGAAGGGATCAGAGGAGGAGGAGAATCTCCACGACCCTTTTTCTAATAGAAAAATTCCTGAAAGTTTACGAAATTTCCTTTCTCTCTCTAAAAGTGTTTTTCTCTCTCCTCTGGGAAGGAAAACCAAAGTGCTGGCAGCTTAAGGTGTTCTTGGGCGGATGTTTCAATTCTTTGCGGCTTTTACGCGCGTTATGTTC
TARGET=1321,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2133
PRIMER_LEFT_PENALTY=0.088662
PRIMER_RIGHT_PENALTY=0.124655
PRIMER_LEFT_SEQUENCE=GACAAAACCCATAAGAACCCAA
PRIMER_RIGHT_SEQUENCE=CATTCTCAATTCCTCTGCTGTG
PRIMER_LEFT=1191,22
PRIMER_RIGHT=1382,22
PRIMER_LEFT_TM=60.089
PRIMER_RIGHT_TM=59.875
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=0.2133
PRIMER_LEFT_1_PENALTY=0.088662
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=TTGACAAAACCCATAAGAACCC
PRIMER_RIGHT_1_SEQUENCE=CATTCTCAATTCCTCTGCTGTG
PRIMER_LEFT_1=1189,22
PRIMER_RIGHT_1=1382,22
PRIMER_LEFT_1_TM=60.089
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=194
PRIMER_PAIR_PENALTY_2=0.2521
PRIMER_LEFT_2_PENALTY=0.127450
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=CCGAAATTGGTTTCTAACCTTG
PRIMER_RIGHT_2_SEQUENCE=CATTCTCAATTCCTCTGCTGTG
PRIMER_LEFT_2=1170,22
PRIMER_RIGHT_2=1382,22
PRIMER_LEFT_2_TM=59.873
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=213
PRIMER_PAIR_PENALTY_3=0.3085
PRIMER_LEFT_3_PENALTY=0.088662
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=GACAAAACCCATAAGAACCCAA
PRIMER_RIGHT_3_SEQUENCE=CTCTGCTGTGATTCCAAACAAC
PRIMER_LEFT_3=1191,22
PRIMER_RIGHT_3=1370,22
PRIMER_LEFT_3_TM=60.089
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.3085
PRIMER_LEFT_4_PENALTY=0.088662
PRIMER_RIGHT_4_PENALTY=0.219884
PRIMER_LEFT_4_SEQUENCE=GACAAAACCCATAAGAACCCAA
PRIMER_RIGHT_4_SEQUENCE=TTTGGTGTCACAGCTTCATTCT
PRIMER_LEFT_4=1191,22
PRIMER_RIGHT_4=1398,22
PRIMER_LEFT_4_TM=60.089
PRIMER_RIGHT_4_TM=59.780
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_01543 Ps_contig_mira-and-tgicl-ass_franssen_14763
SEQUENCE=CTCCAAACTTACAGGTCATCCTCAAAATTAATATCAAAATACAAAATTCCGCAGAAGTTCGGGAATCGGAAAACCACCATCATCTGCACGCATTGATCCCATTTCAACATCAAGTTTCTCCTTTGGTGTCAAAATTTCGTCCACAATATTCGGAGTCAATGAACCAGTTGTCATATGTTCATCTTGCTGTACAAATGATCGGAAATTATTCTTTGAAGCGGGAATACTGGAACAGAGTGTTTCTCCAATATTTCTCACTGATCCCCTGTTGTATGGATTCCCCTTTTTATCATATTGGTACCGGAAGTTTTCATAAGTTGTCTGATTTGTACAAATGAGATAGAAATGGAAAGCTGTGAGGCCCCCGACAAACCAGACAGCAATGAAGCAATATATAATGAGAAAATCCGATACATAATCATGTGAGATTGCGTCCCAAACATTGTTCCTGGTCTTGGAAAGATTGACCCAAGAGAACACAAAGACATATAGGCACAAAAAGGTTGATGTTGTAATAAACATGAAGAAGTGCCGATAGTTTCGTCTTCCGATGCACTGGCCAACCCAGGGACAATGATGATCAAATCTCTGGACACAGTTGTTGCATATAGAACAATGAGAAGTACGAGGGGGTCGATAAAGCAAGCATGTATCACAAAACTTCACTTTCACCGTGTAACCATTAACCATTACATCTTTAGTTCGAGGTAGTTTCAAATGAGGGGTTGAACCATTAATCCACTCCATGGAAGGAGTAGGTATATTAAATGTTTCGTCGCATTCTGGAGGTGTTGAGTTTCTAGGAATAATTCCAGGATCTCTCGCGGAAGTCAAGAGGAGGAATAGGAAATCCAGAATAGTGAGAGTTAGACCGACAATCACTACGGGACACCAAAAAACATGACCACTACTTGCATTCTCATTCTTGGCTTTAAGATACATCTTGACACAAAATGTAATCGCAGGTGCAGCAATTAGGAACGATGTAAGGAACAAGGAAGCTACATCAGGACCAAAAACCAGTCTCCCACCACAGAGAAACTTATTGCCTCCTCTCCAAACTTGATAGAGCCTCCTCGGTTTCATATCATGGGCCCCAACTCCAGAAGGAGGAGACCCAAATTGCTGACTTCCATTCCTAATCATTGCAGCAGCACCACACGTGTGTCACCGAAATTGGTTTCTAACCTTGACAAAACCCATAAGAACCCAAATCAAGCAAGCTTCAACCCCGATTCAGAATCTTGTTAAAAATCAAAAATTTCGATCAGGGGTTCCTACAATTATCCTATTTCTGCAGTGAGAATGGAGAATGCAGATGAGAGAGAGAGAGAGAGAAAGAGAAGTTTGTTGTTTGGAATCACAGCAGAGGAATTGAGAATGAAGCTGTGACACCAAATTACAAACAGGAAAATTTGATCGAATGAAGGGATCAGAGGAGGAGGAGAATCTCCACGACCCTTTTTCTAATAGAAAAATTCCTGAAAGTTTACGAAATTTCCTTTCTCTCTCTAAAAGTGTTTTTCTCTCTCCTCTGGGAAGGAAAACCAAAGTGCTGGCAGCTTAAGGTGTTCTTGGGCGGATGTTTCAATTCTTTGCGGCTTTTACGCGCGTTATGTTC
TARGET=1436,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4491
PRIMER_LEFT_PENALTY=0.095924
PRIMER_RIGHT_PENALTY=0.353222
PRIMER_LEFT_SEQUENCE=TGGAGAATGCAGATGAGAGAGA
PRIMER_RIGHT_SEQUENCE=TCCCAGAGGAGAGAGAAAAACA
PRIMER_LEFT=1306,22
PRIMER_RIGHT=1539,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=60.353
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.4779
PRIMER_LEFT_1_PENALTY=0.124655
PRIMER_RIGHT_1_PENALTY=0.353222
PRIMER_LEFT_1_SEQUENCE=CACAGCAGAGGAATTGAGAATG
PRIMER_RIGHT_1_SEQUENCE=TCCCAGAGGAGAGAGAAAAACA
PRIMER_LEFT_1=1361,22
PRIMER_RIGHT_1=1539,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.353
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=179
PRIMER_PAIR_PENALTY_2=0.5500
PRIMER_LEFT_2_PENALTY=0.196811
PRIMER_RIGHT_2_PENALTY=0.353222
PRIMER_LEFT_2_SEQUENCE=CGATCAGGGGTTCCTACAATTA
PRIMER_RIGHT_2_SEQUENCE=TCCCAGAGGAGAGAGAAAAACA
PRIMER_LEFT_2=1264,22
PRIMER_RIGHT_2=1539,22
PRIMER_LEFT_2_TM=60.197
PRIMER_RIGHT_2_TM=60.353
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.5731
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=0.353222
PRIMER_LEFT_3_SEQUENCE=AGAATGAAGCTGTGACACCAAA
PRIMER_RIGHT_3_SEQUENCE=TCCCAGAGGAGAGAGAAAAACA
PRIMER_LEFT_3=1377,22
PRIMER_RIGHT_3=1539,22
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=60.353
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=163
PRIMER_PAIR_PENALTY_4=0.5731
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=0.353222
PRIMER_LEFT_4_SEQUENCE=GTTGTTTGGAATCACAGCAGAG
PRIMER_RIGHT_4_SEQUENCE=TCCCAGAGGAGAGAGAAAAACA
PRIMER_LEFT_4=1349,22
PRIMER_RIGHT_4=1539,22
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=60.353
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_01544 Ps_contig_mira-and-tgicl-ass_franssen_14764
SEQUENCE=GAGAGGAGAGAGAAAAACACTTTTAGAGAGAGAAAGGAAATTTCGTAAACTTTCAGGAATTTTTTCTTATTAGAAAAAAGGGTACGTGGAGATTCTCCTCCTCCTCTGATCCCTTCATTCGATCAAATTTTCCTGTTGTAATTTGGTGTCACAGCTTCATTCTCAATTCCTCTGCTGTGATTCCAAACAACAAACTTCTCTTTCTCTCTCTCTCTCATCTGCATTCTCCATTCTCA
TARGET=95,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.8755
PRIMER_LEFT_PENALTY=3.779535
PRIMER_RIGHT_PENALTY=0.095924
PRIMER_LEFT_SEQUENCE=AAAAAGGGTACGTGGAGATTC
PRIMER_RIGHT_SEQUENCE=TGGAGAATGCAGATGAGAGAGA
PRIMER_LEFT=74,21
PRIMER_RIGHT=229,22
PRIMER_LEFT_TM=57.220
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=3.9042
PRIMER_LEFT_1_PENALTY=3.779535
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=AAAAAGGGTACGTGGAGATTC
PRIMER_RIGHT_1_SEQUENCE=CACAGCAGAGGAATTGAGAATG
PRIMER_LEFT_1=74,21
PRIMER_RIGHT_1=178,22
PRIMER_LEFT_1_TM=57.220
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=3.9994
PRIMER_LEFT_2_PENALTY=3.779535
PRIMER_RIGHT_2_PENALTY=0.219884
PRIMER_LEFT_2_SEQUENCE=AAAAAGGGTACGTGGAGATTC
PRIMER_RIGHT_2_SEQUENCE=GTTGTTTGGAATCACAGCAGAG
PRIMER_LEFT_2=74,21
PRIMER_RIGHT_2=190,22
PRIMER_LEFT_2_TM=57.220
PRIMER_RIGHT_2_TM=59.780
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=4.1275
PRIMER_LEFT_3_PENALTY=3.779535
PRIMER_RIGHT_3_PENALTY=0.347935
PRIMER_LEFT_3_SEQUENCE=AAAAAGGGTACGTGGAGATTC
PRIMER_RIGHT_3_SEQUENCE=GCAGAGGAATTGAGAATGAAGC
PRIMER_LEFT_3=74,21
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=57.220
PRIMER_RIGHT_3_TM=60.348
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=4.3270
PRIMER_LEFT_4_PENALTY=3.779535
PRIMER_RIGHT_4_PENALTY=0.547508
PRIMER_LEFT_4_SEQUENCE=AAAAAGGGTACGTGGAGATTC
PRIMER_RIGHT_4_SEQUENCE=CAGCAGAGGAATTGAGAATGAA
PRIMER_LEFT_4=74,21
PRIMER_RIGHT_4=176,22
PRIMER_LEFT_4_TM=57.220
PRIMER_RIGHT_4_TM=59.452
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_01545 Ps_contig_mira-and-tgicl-ass_franssen_14764
SEQUENCE=GAGAGGAGAGAGAAAAACACTTTTAGAGAGAGAAAGGAAATTTCGTAAACTTTCAGGAATTTTTTCTTATTAGAAAAAAGGGTACGTGGAGATTCTCCTCCTCCTCTGATCCCTTCATTCGATCAAATTTTCCTGTTGTAATTTGGTGTCACAGCTTCATTCTCAATTCCTCTGCTGTGATTCCAAACAACAAACTTCTCTTTCTCTCTCTCTCTCATCTGCATTCTCCATTCTCA
TARGET=203,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01546 Ps_contig_mira-and-tgicl-ass_franssen_14769
SEQUENCE=CTATGGTGCGCTGAGGAGGAACACTGTCCCTTGCTCACGACGCGGTGCTTCTTATTATAATTGTCGACCTGGTGCTCAGGCGAATCCCTACCACCGTGGATGCAGTGCCATCACTCGATGCAGGCGTTGAAGTTATTATATTCTATTTTCTCCTAATTTAYTTTGTTTATTTTCTATTTTTTATATATATATATTTNTATGATGATAATAGATCTTGTTGTTATGTACTATATAATAGTATAGTATAAG
TARGET=182,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01547 Ps_contig_mira-and-tgicl-ass_franssen_14770
SEQUENCE=GWYKSKGAACTCGAGTAAACAAATCAAACATGGGAACCAGAATCAGCTTCTTTCTTATCTGTGCAATCTTAGCCGTCCACGTGGAGTTTTCGTTATCCTTTACCCTTGATTTCACCGACAACCGTCTCGGCAACTTCTTCCTTCCGATGAACACCGGCTGCCGTGGATCCATCGCCGAATGTAGCCTTGAAGATACCGAGTTTCTGATGGAATCTGAGAGCAACAGGCGCATTTTAGCAGCAACAAAGTACATAAGCTATGGTGCGCTGAGGAGGAACACTGTCCCTTGCTCACGACGCGGTGCTTCTTATTATAATTGTCGACCTGGTGCTCAGGCGAATCCCTACCACCGTGGATGCAGTGCCATCACTCGATGCAGGCGTTGAAGTTATTATATTCATTTTCTCTAATTTATTTTGTTATTTTCATTTTTTTATATATATAATTTTGTAATTGATGATAATAGATCTTGTTGTTATGTACTACTAGTAATAGTATAGTATAGAGAAAATTGAAGTAGTAGAGTTGAATAAAATGAAGTTCCATGCCGTTTGTATTATTCATTTGAGACTTGAGAAGATTACTATCATCAATAATTTATATTGTTAATTCTTGTTGATTAMTTT
TARGET=435,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01548 Ps_contig_mira-and-tgicl-ass_franssen_14801
SEQUENCE=GTCGGGTTTTATTTAGAAAGTTTTCGATTTAAGAAAGAATAGAACAAACTATATATATTTCTAAGAGAGAACCATTTCCCTACAAATCAAATATAAGTAAGGGTTCGATGATTTTGGAAGTTTCTATTTTGTCCTTAATATTTATGTGTCACCCAACCAATCTTCTTTTCTTTGTCAAAACTCTCTCTCTCTTATTACATTCACTCGTCTTCTATTTTTTTTCCTTATGTTTCCAACTTTTTTCTCTTTCACTTCTATTTTTTTCTTCAATCGAAATTTCTCTCTTATATCAAACTCTCTGTCTTTCTCTTTCTCATCACCACTTTTTCCACGGTCATTTCAACTTCTTTTTCAACTTTTTTCCTTCTGATTTGTCATACGGTTGTGTTTTGCAGATCCATTCAAAATTGTTCATTTTCATCAAGAAAATAATAAAGAATAAGAATAGGTCTAATAAAAGTTGTTTATCTTTATCAAGAAAAAAATAAAGAACAAGAGATTTCTAGGTTGAAAAGAAGGTTGGTG
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3418
PRIMER_LEFT_PENALTY=0.267873
PRIMER_RIGHT_PENALTY=0.073900
PRIMER_LEFT_SEQUENCE=GTGTCACCCAACCAATCTTCTT
PRIMER_RIGHT_SEQUENCE=ATCTGCAAAACACAACCGTATG
PRIMER_LEFT=145,22
PRIMER_RIGHT=396,22
PRIMER_LEFT_TM=60.268
PRIMER_RIGHT_TM=59.926
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.4060
PRIMER_LEFT_1_PENALTY=0.332137
PRIMER_RIGHT_1_PENALTY=0.073900
PRIMER_LEFT_1_SEQUENCE=TCACCCAACCAATCTTCTTTTC
PRIMER_RIGHT_1_SEQUENCE=ATCTGCAAAACACAACCGTATG
PRIMER_LEFT_1=148,22
PRIMER_RIGHT_1=396,22
PRIMER_LEFT_1_TM=60.332
PRIMER_RIGHT_1_TM=59.926
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=0.4821
PRIMER_LEFT_2_PENALTY=0.408180
PRIMER_RIGHT_2_PENALTY=0.073900
PRIMER_LEFT_2_SEQUENCE=TTTATGTGTCACCCAACCAATC
PRIMER_RIGHT_2_SEQUENCE=ATCTGCAAAACACAACCGTATG
PRIMER_LEFT_2=140,22
PRIMER_RIGHT_2=396,22
PRIMER_LEFT_2_TM=59.592
PRIMER_RIGHT_2_TM=59.926
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.5685
PRIMER_LEFT_3_PENALTY=0.183834
PRIMER_RIGHT_3_PENALTY=0.384647
PRIMER_LEFT_3_SEQUENCE=GTAAGGGTTCGATGATTTTGGA
PRIMER_RIGHT_3_SEQUENCE=AAAGAAGTTGAAATGACCGTGG
PRIMER_LEFT_3=96,22
PRIMER_RIGHT_3=349,22
PRIMER_LEFT_3_TM=60.184
PRIMER_RIGHT_3_TM=60.385
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.5745
PRIMER_LEFT_4_PENALTY=0.500551
PRIMER_RIGHT_4_PENALTY=0.073900
PRIMER_LEFT_4_SEQUENCE=CACCCAACCAATCTTCTTTTCT
PRIMER_RIGHT_4_SEQUENCE=ATCTGCAAAACACAACCGTATG
PRIMER_LEFT_4=149,22
PRIMER_RIGHT_4=396,22
PRIMER_LEFT_4_TM=59.499
PRIMER_RIGHT_4_TM=59.926
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_01549 Ps_contig_mira-and-tgicl-ass_franssen_14802
SEQUENCE=ATGTGTTTTTTCATAGTGAAAAACTAACCTGATCTTTAAAATCAAATTCAACTTATCAATAAGATTTCAACCATTAATTTAAATTAACATAAATGAATATAATATTATGTTTACCTTATTTTCTTTTATAGTATCACTATCCTATTTTATCTATTTTTCTTTAATGATTATTATGTATGAATAATTTTTTATAGAAAGTTTTCGATTTAAGAAAGAATAGAACAAACTATATATATTTCTAAGAGAGAACCATTTCCCTACAAATCAAATATAAGTAAGGGTTCGATGATTTTTGGAAGTTTYYTATTTTGTTACCTTAATTATTTATGTGTCACCCAACCTAATCTTTCTTTTCTTGTACAAACTCTCTCTCTCTTA
TARGET=365,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01550 Ps_contig_mira-and-tgicl-ass_franssen_14803
SEQUENCE=ACGGGGATCGGATTATGAAATCAAATCGATGTGTTTCACAAAAGVGGGGGTATAATCCTAGTCGCATCGAGTAGAAAGGGTTAACCAAAGTCAAAACCCATAGAGGAAGTTATGTTGAGAGCATCACCATCATTCTTTCGATTAACCACCACCACCACCACCACCACCAYCAGAATGTTCTCTTCTACTTCTTCCATTTCTTCTCTCAGAAACCCTAACTTCCTCTTCCCTTCAACTTCATCACCGCATTCTTCAAAACTCTCTCTTCCTCCTCACTCTCTTTCGTTTTCCAACAATCGCAATCGCAATCGCATCTCCTTTCGATGCTTCGCTTCTCGTCCCATTCATGTCGTCAATCCCATCGTCGAAATGGACGGTGACG
TARGET=145,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01551 Ps_contig_mira-and-tgicl-ass_franssen_14804
SEQUENCE=AAGGAGATGCGATTGCGATTGCGATTGTTGGAAAACGAAAGAGAGTGAGGAGGAAGAGAGAGTTTTGAAGAATGCGGTGATGAAGTTGAAGGGAAGAGGAAGTTAGGGTTTCTGAGAGAAGAAATGGAAGAAGTAGAAGAGAACATTCTGGTGGTGGTGGTGGTGGTGGTGGTTAATCGAAAGAATGATGGTGATGCTCTCAACATAACCTTCC
TARGET=149,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4424
PRIMER_LEFT_PENALTY=0.142170
PRIMER_RIGHT_PENALTY=0.300243
PRIMER_LEFT_SEQUENCE=TGAAGTTGAAGGGAAGAGGAAG
PRIMER_RIGHT_SEQUENCE=TGAGAGCATCACCATCATTCTT
PRIMER_LEFT=80,22
PRIMER_RIGHT=201,22
PRIMER_LEFT_TM=59.858
PRIMER_RIGHT_TM=59.700
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=122
PRIMER_PAIR_PENALTY_1=0.4424
PRIMER_LEFT_1_PENALTY=0.142170
PRIMER_RIGHT_1_PENALTY=0.300243
PRIMER_LEFT_1_SEQUENCE=TGAAGTTGAAGGGAAGAGGAAG
PRIMER_RIGHT_1_SEQUENCE=TTGAGAGCATCACCATCATTCT
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=202,22
PRIMER_LEFT_1_TM=59.858
PRIMER_RIGHT_1_TM=59.700
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=123
PRIMER_PAIR_PENALTY_2=0.5634
PRIMER_LEFT_2_PENALTY=0.124370
PRIMER_RIGHT_2_PENALTY=0.439061
PRIMER_LEFT_2_SEQUENCE=CGAAAGAGAGTGAGGAGGAAGA
PRIMER_RIGHT_2_SEQUENCE=GTTGAGAGCATCACCATCATTC
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=203,22
PRIMER_LEFT_2_TM=60.124
PRIMER_RIGHT_2_TM=59.561
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.5812
PRIMER_LEFT_3_PENALTY=0.142170
PRIMER_RIGHT_3_PENALTY=0.439061
PRIMER_LEFT_3_SEQUENCE=TGAAGTTGAAGGGAAGAGGAAG
PRIMER_RIGHT_3_SEQUENCE=GTTGAGAGCATCACCATCATTC
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=203,22
PRIMER_LEFT_3_TM=59.858
PRIMER_RIGHT_3_TM=59.561
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=0.6097
PRIMER_LEFT_4_PENALTY=0.309418
PRIMER_RIGHT_4_PENALTY=0.300243
PRIMER_LEFT_4_SEQUENCE=GATGAAGTTGAAGGGAAGAGGA
PRIMER_RIGHT_4_SEQUENCE=TGAGAGCATCACCATCATTCTT
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=201,22
PRIMER_LEFT_4_TM=59.691
PRIMER_RIGHT_4_TM=59.700
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_01552 Ps_contig_mira-and-tgicl-ass_franssen_14805
SEQUENCE=GATTCACATAACATCTTCGTCTCTCATCAATCATCATTCAACTACTCTTTGATATATATAATTCTAATCAACTTCATCATCATCAACAACATCATGAGTAACTTTAACAATCAGCAACAAGCAGCACCTATATCATATCCACCACCAGGTGAGGCGTATTCTACTTCTCAATATGTAACTGCCCCACCACCTATGGGTTACCCTTCCCAGGATGCCCCTCAACAAAGGATTCCAGATCAAACAACGACCAGAGGTGACGGTTT
TARGET=74,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1451
PRIMER_LEFT_PENALTY=2.107002
PRIMER_RIGHT_PENALTY=0.038090
PRIMER_LEFT_SEQUENCE=CTTCGTCTCTCATCAATCATCA
PRIMER_RIGHT_SEQUENCE=ACCTCTGGTCGTTGTTTGATCT
PRIMER_LEFT=14,22
PRIMER_RIGHT=254,22
PRIMER_LEFT_TM=57.893
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=2.1625
PRIMER_LEFT_1_PENALTY=2.102638
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=CATCTTCGTCTCTCATCAATCATC
PRIMER_RIGHT_1_SEQUENCE=GATCTGGAATCCTTTGTTGAGG
PRIMER_LEFT_1=11,24
PRIMER_RIGHT_1=237,22
PRIMER_LEFT_1_TM=60.103
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=227
PRIMER_PAIR_PENALTY_2=2.1669
PRIMER_LEFT_2_PENALTY=2.107002
PRIMER_RIGHT_2_PENALTY=0.059875
PRIMER_LEFT_2_SEQUENCE=CTTCGTCTCTCATCAATCATCA
PRIMER_RIGHT_2_SEQUENCE=GATCTGGAATCCTTTGTTGAGG
PRIMER_LEFT_2=14,22
PRIMER_RIGHT_2=237,22
PRIMER_LEFT_2_TM=57.893
PRIMER_RIGHT_2_TM=59.940
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=2.4284
PRIMER_LEFT_3_PENALTY=2.102638
PRIMER_RIGHT_3_PENALTY=0.325733
PRIMER_LEFT_3_SEQUENCE=CATCTTCGTCTCTCATCAATCATC
PRIMER_RIGHT_3_SEQUENCE=AAGTAGAATACGCCTCACCTGG
PRIMER_LEFT_3=11,24
PRIMER_RIGHT_3=165,22
PRIMER_LEFT_3_TM=60.103
PRIMER_RIGHT_3_TM=59.674
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=2.4327
PRIMER_LEFT_4_PENALTY=2.107002
PRIMER_RIGHT_4_PENALTY=0.325733
PRIMER_LEFT_4_SEQUENCE=CATCTTCGTCTCTCATCAATCA
PRIMER_RIGHT_4_SEQUENCE=AAGTAGAATACGCCTCACCTGG
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=165,22
PRIMER_LEFT_4_TM=57.893
PRIMER_RIGHT_4_TM=59.674
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_01553 Ps_contig_mira-and-tgicl-ass_franssen_14806
SEQUENCE=TAAGTGGGATTCACATAACATCTTCTCCTCTCATCAATCATCATTCAACTACTCTTTGATATATATAATTCTAATCATCTTCATCAACAACAACAACATCATGAGTAACTTTAACAATCAGCAACAAGCACCTATATCATATCCACCACCAGGTGAGGCTTATTCGACTTCTCAATATGTAACTGCCCCACCACCTATGGGTTACCCCTTCCCAGGATGCCCCTCAACAAAGGATTCCAGATCAAACAACAACCAGAGGTGATGGTTTCGTGGGAAGGGATGTTGTGC
TARGET=85,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1694
PRIMER_LEFT_PENALTY=2.161164
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=GGGATTCACATAACATCTTCTCC
PRIMER_RIGHT_SEQUENCE=CTGGTTGTTGTTTGATCTGGAA
PRIMER_LEFT=5,23
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=58.839
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=2.2210
PRIMER_LEFT_1_PENALTY=2.161164
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=GGGATTCACATAACATCTTCTCC
PRIMER_RIGHT_1_SEQUENCE=GATCTGGAATCCTTTGTTGAGG
PRIMER_LEFT_1=5,23
PRIMER_RIGHT_1=242,22
PRIMER_LEFT_1_TM=58.839
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=2.2615
PRIMER_LEFT_2_PENALTY=2.253194
PRIMER_RIGHT_2_PENALTY=0.008257
PRIMER_LEFT_2_SEQUENCE=GGGATTCACATAACATCTTCTCCT
PRIMER_RIGHT_2_SEQUENCE=CTGGTTGTTGTTTGATCTGGAA
PRIMER_LEFT_2=5,24
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.008
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=2.3131
PRIMER_LEFT_3_PENALTY=2.253194
PRIMER_RIGHT_3_PENALTY=0.059875
PRIMER_LEFT_3_SEQUENCE=GGGATTCACATAACATCTTCTCCT
PRIMER_RIGHT_3_SEQUENCE=GATCTGGAATCCTTTGTTGAGG
PRIMER_LEFT_3=5,24
PRIMER_RIGHT_3=242,22
PRIMER_LEFT_3_TM=59.747
PRIMER_RIGHT_3_TM=59.940
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=2.3485
PRIMER_LEFT_4_PENALTY=2.161164
PRIMER_RIGHT_4_PENALTY=0.187326
PRIMER_LEFT_4_SEQUENCE=GGGATTCACATAACATCTTCTCC
PRIMER_RIGHT_4_SEQUENCE=TAGGTGCTTGTTGCTGATTGTT
PRIMER_LEFT_4=5,23
PRIMER_RIGHT_4=133,22
PRIMER_LEFT_4_TM=58.839
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=129
=
PRIMER_SEQUENCE_ID=SSR_01554 Ps_contig_mira-and-tgicl-ass_franssen_14807
SEQUENCE=TCAACTCMCAATCCCGTGAAGCTTGCGGATGTTAAAACTGAAGTTGAAGCTGACGGCTCCGCTGAGAAGGTAACTGAGAAGCTGAAGGAGATCAAAGTTGAAGTGTGCGAAGGTTCGCATTCTGCTTCAATTCATATATCAGCAGCAACAGCAACAGCTGGTGCATCTCAAGAGCAATCTGCTCTACAGAACAATAAGGATGCGGGTATCCCCATAAGTAAAAAGAAGCGCGGGGAACGCAAGGCTGGTTCAGGTGGGTGGAACAAGTAGTCCCCCCGGAGAAGCCGATAAACAATAAGACTTAGCTGGTGAATAATAATAATAATACTTCACAATTTGTTGTGAAAACCGGTGAATAATACTTCACAATTTGTTGTGACATAATAGTTTCAAGCTCATTTTTATCAGGGTTGTTGTGACATTTATTTAAACAGTTTTAGCCGTGATATTGAATATGGTTTTATTAGAGAATATGATGGATCAGTAGCTGTAAGTGTCTTTGGGATAAGGGGTTGAGTGAGAGAATGGTGGGGGTGTATCTTGGAATTGAAATTTAGTTTAGTTAGTTATGGATAGGAGGCAATAGCTTTCTATAGGAGCATAAGCTCTGAGGTAGTCAGGGTTTTCCCGTCATTTCTCTGTAACTTCCCCTGTTCACATTAANAAATAACACTCTAAATTT
TARGET=312,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01555 Ps_contig_mira-and-tgicl-ass_franssen_14820
SEQUENCE=AAACGAATGTAAGAAGAAGTATAAGGAGTAAGAATNAGGAGTTGGGGTTCGGTTGGGTCGTCAGAGATTAGAGCACCGAATCAAAAAACAAAGAATATGGTTAGGTTCTTATTCAACTCCAGAAGCTGCTGCTAGAGCTTATGATGCTGCACTTTTGTGCCTTAAAGGTTCATCTTCAGCTTCAAATCTCAATTTCCCTTTAACAGTCTCTTCTTCGGTTTCGCATTGCATTATTCCTCAAGATATGTCTCCGAAATCAATTCAAAGAGTTGCTGCAGCTGCTGCTAATAATTTTAATCTCAATGTCACTCCCTCTTCATCATCATCATCATTGGTTTCATCTCCATCAATGGGTTCTTCTTCGGATGATGTTTCAATTAGGTCATCTTCTGATCAAGCTAACCGTGAAATGATGGATATGATGGAGACTAATTGGTATGGATTAGAAGGTTTAGAATCTCCTAAATATGTTGATCAGGTGTTGTTAAGTGCTTCTTTCTTTGATGTTGAATATTCATCATATTTGCTTGGTGATGTGTATGAAGAAAGTGATATTCGTTTATGGAACTTCAACTGAAGAAACTTAAATCCTTTTTCTTGTTTTTTTCTCCTTAAAACTCAATTCAAATTATTTTCTCTTCAGTGATACTACTTGAAGATGTTAGTGTAAGAAATAAATCTATGGCTCTAATGCTATATATTTGAATATATTGTCAGAGCTCAAATTTGTATGTCTTTTTTGATTTGTTGAAATGGAAGTATAAATATTTTCCTCWAWMWAYTCATGTCATGAC
TARGET=317,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01556 Ps_contig_mira-and-tgicl-ass_franssen_14833
SEQUENCE=GCAAACGAATGCAGAAAACGAAATGCAGAAACGACACGGGYGCAGCATCAGTACGCAAAAGTACGAACAACAACAACGCCAAAATGAAGGAAACGACACAAAGCAAAAATAAAACAAACTAGCAGTAACAATTACAAAACGACAGTAGAAACCGAAGCTTCCAAAAAGCCTTCACAACATTATCCTGTTATCACCAGAAAGCCACTGTATCGGTAAGTTACTTTTTTTGTCATCT
TARGET=66,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01557 Ps_contig_mira-and-tgicl-ass_franssen_14871
SEQUENCE=TTTGCCGAAATCCATTAACCTAGTAAATGATTTTATCAACAGTGGAATAAACATTTTATATTACAATTTTACTTGTAGTCATAACATGAGGAAATATACTGAAGTTGAATAAGCAATGACAAAATTTAAAATAGATAAAGAGGAGTACTTCCTGTACATAGTGCACATCTCAAAGCATGACTAAATCTATATAACACAAATGGAATGAATGGATTGAGGGCAAAAGGAAATGCTACCAGTTATCCTTAACAATTTTTGACTTTTCAAGGATGAATTTTCCTTCTTTGTACTTCTGGGAAGCATCATAAGCTCTTTCGTATTTCCAACCCAGAACTTCTCGGCAATCACCGCAGTAAACATCCGCAACGGTGTGGAGGCCAGTCAAGAGATGCCTGTCCTCCTTTGGCCCAGTGATAACATTCATGGCATGGGAAAACAGAAAGGCTCGGCCGTTTTTTCCCTGAAAAGCCTTTGAAATGATATCATCGTGGAGTGAAACATGGTTTCTACAATTACAGCAACTGTACAAACGAGGCACTATGAGTTCCGCCATGAATAATTTGGTAACTGTATTTCAGATTGAAAATAACTCAGATGCGTGACCAATACGATAGAGTCTGCAATAACAACAAGATAAGAATGAATGATACAGAGAAAAAGATAGCGTGCAAGTACTAATTAATTAATTCATTCATCACACAGATAACTAAATATTCTTTCTCTAAATAAATAATATCTAATCAGATAAAGACATAGCCACTTATAAATAGCATAGCGAGCCAACGCTCAGACTCMAATGACGAGAGTGGAACTTCC
TARGET=676,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01558 Ps_contig_mira-and-tgicl-ass_franssen_14875
SEQUENCE=CTGAAATTAGCTTGCTGTCACCTCCTTAGAATCTTAATCTGAGAAATCAACTCTGATCGTTCCGACCGCTTTGCCTTCAGTGCTTCAGTGATCTGAAACCGAGCTTGATTTTTCTTGCTGATCTCCTTATCAAGGAGATCAATCTTCGTTTTGATGGATGGATCATCATAAGGCCTGCACCTGACAAAATAGAACGAATGAATCTGCTTAGGCGCAGGCCACTCTTCAGCAGCGTTCTTCGGGGTATTGGAATCAGCAACATCGTCAGCTTCCCCTTTAGCAGATTCATCTCCATGTGAGCCAAATTTGATGGCCTCACCAGCTCCCAGATCTTGTTCCAGCTTTCCATTCTCCTTTTCATGTGCAACAGATTTGCCTCCTTCAACACCATTCTCAATCGGTCCTTGAACCATCTCGAATCCCGCAATCTCAACCGCCATGACTCCAACTACAAAATCCTACAAACCAGTGTAGAGAGGTAAGGAGATTTGTAGAGAAGAAGACCCCTTTTGACAAAGGGTCTCCGAATTCAATTCCAAATTATGTAAAAACCCAAAAAATTGAGATTGAAAAAATGGGTCAGATCTCGTGATTTCTTCTTCCTTAGTCCTCCACGTGCAGGTTTAATCAGAACCCGTTTCGCAGAGAGGGAGAGAGAGAGAAACAGGGAGGGGAGAGAGAAACAGAAAGAGAC
TARGET=651,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3454
PRIMER_LEFT_PENALTY=0.270774
PRIMER_RIGHT_PENALTY=1.074607
PRIMER_LEFT_SEQUENCE=GAGAAGAAGACCCCTTTTGACA
PRIMER_RIGHT_SEQUENCE=TTTCTGTTTCTCTCTCCCCTCC
PRIMER_LEFT=493,22
PRIMER_RIGHT=688,22
PRIMER_LEFT_TM=59.729
PRIMER_RIGHT_TM=61.075
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=1.4679
PRIMER_LEFT_1_PENALTY=0.270774
PRIMER_RIGHT_1_PENALTY=1.197140
PRIMER_LEFT_1_SEQUENCE=GAGAAGAAGACCCCTTTTGACA
PRIMER_RIGHT_1_SEQUENCE=TTCTGTTTCTCTCTCCCCTCC
PRIMER_LEFT_1=493,22
PRIMER_RIGHT_1=687,21
PRIMER_LEFT_1_TM=59.729
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=1.4933
PRIMER_LEFT_2_PENALTY=0.418705
PRIMER_RIGHT_2_PENALTY=1.074607
PRIMER_LEFT_2_SEQUENCE=GGGTCAGATCTCGTGATTTCTT
PRIMER_RIGHT_2_SEQUENCE=TTTCTGTTTCTCTCTCCCCTCC
PRIMER_LEFT_2=576,22
PRIMER_RIGHT_2=688,22
PRIMER_LEFT_2_TM=59.581
PRIMER_RIGHT_2_TM=61.075
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=1.6158
PRIMER_LEFT_3_PENALTY=0.418705
PRIMER_RIGHT_3_PENALTY=1.197140
PRIMER_LEFT_3_SEQUENCE=GGGTCAGATCTCGTGATTTCTT
PRIMER_RIGHT_3_SEQUENCE=TTCTGTTTCTCTCTCCCCTCC
PRIMER_LEFT_3=576,22
PRIMER_RIGHT_3=687,21
PRIMER_LEFT_3_TM=59.581
PRIMER_RIGHT_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=112
PRIMER_PAIR_PENALTY_4=1.6258
PRIMER_LEFT_4_PENALTY=0.270774
PRIMER_RIGHT_4_PENALTY=1.355029
PRIMER_LEFT_4_SEQUENCE=GAGAAGAAGACCCCTTTTGACA
PRIMER_RIGHT_4_SEQUENCE=TCTTTCTGTTTCTCTCTCCCCTC
PRIMER_LEFT_4=493,22
PRIMER_RIGHT_4=690,23
PRIMER_LEFT_4_TM=59.729
PRIMER_RIGHT_4_TM=60.355
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=198
=
PRIMER_SEQUENCE_ID=SSR_01559 Ps_contig_mira-and-tgicl-ass_franssen_14885
SEQUENCE=AAGCGGTGGTTAATTCAGCCGTTATCAGAGCTTCTGGAATGCTCCAGATGAAAGGGAAGATGAGGAAGCCGAGAAGGGCGAAGAGAGGACCGGCGGCCTGCACGGCGGGTTCTTCGCCGTATGGGCCGCCGGCTACTTCGAAGTAGATGAGGAATATTAGAGGGATTAGGGTTAGTTTTTTGGTGGGTTTTATGGGAGTGGTTACTTGTGCTGGTTCCGGCGGTGTGGTGGTGGTGGTGGTGGTGGCGGTGGCGGTGGAAGGAACAGCTTCGCCCATTGATGAGTTAGTTGAATGATTGGAATTTGATAGTGGCAGATATATAGATTCACTTTCACGCATGAATAGAAATACCCCCGAC
TARGET=225,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5224
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.502561
PRIMER_LEFT_SEQUENCE=GTGGGTTTTATGGGAGTGGTTA
PRIMER_RIGHT_SEQUENCE=ACTAACTCATCAATGGGCGAAG
PRIMER_LEFT=182,22
PRIMER_RIGHT=288,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.503
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=0.5224
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.502561
PRIMER_LEFT_1_SEQUENCE=TGGGTTTTATGGGAGTGGTTAC
PRIMER_RIGHT_1_SEQUENCE=ACTAACTCATCAATGGGCGAAG
PRIMER_LEFT_1=183,22
PRIMER_RIGHT_1=288,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=60.503
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=0.7559
PRIMER_LEFT_2_PENALTY=0.253332
PRIMER_RIGHT_2_PENALTY=0.502561
PRIMER_LEFT_2_SEQUENCE=CCGTTATCAGAGCTTCTGGAAT
PRIMER_RIGHT_2_SEQUENCE=ACTAACTCATCAATGGGCGAAG
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.503
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=0.7602
PRIMER_LEFT_3_PENALTY=0.257608
PRIMER_RIGHT_3_PENALTY=0.502561
PRIMER_LEFT_3_SEQUENCE=GGTGGTTAATTCAGCCGTTATC
PRIMER_RIGHT_3_SEQUENCE=ACTAACTCATCAATGGGCGAAG
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=288,22
PRIMER_LEFT_3_TM=59.742
PRIMER_RIGHT_3_TM=60.503
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.8690
PRIMER_LEFT_4_PENALTY=0.019805
PRIMER_RIGHT_4_PENALTY=0.849238
PRIMER_LEFT_4_SEQUENCE=GTGGGTTTTATGGGAGTGGTTA
PRIMER_RIGHT_4_SEQUENCE=AACTAACTCATCAATGGGCGAA
PRIMER_LEFT_4=182,22
PRIMER_RIGHT_4=289,22
PRIMER_LEFT_4_TM=59.980
PRIMER_RIGHT_4_TM=60.849
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_01560 Ps_contig_mira-and-tgicl-ass_franssen_14903
SEQUENCE=AATTTTTTTTTCTTAAAGAAAATTTAACATTCAAAATAGAGCCATTACATTCAAATCCCAACTTACATTGTTAATACAAAAAAARRAGATTTTGGTAAGGTTGACCTTTTAGTCAATTATTGACTTTTTGGTCAACAGTCGACCAAAGTCAACAACACATACTTCTAAAACCCTATTTCTCATTTTTTCCATCATCTCTTTTGACTCTCATCCAAAGCTTGCTTCAATCACAAGCAAGCCATGTTTGCATTCTCCACCTCCATTGAACTTTCATCATATGCACACTTTGAAGTCATGATCCAATGCAAGCAAATCAGCCTTGGGCAGCCCTCGTTAAAGCACTCATTGGTTTCTTGTTTCAAAACTGCATCTTCTTCATTGCAACAATATCCGGAGCAAGCCACCGATTTTGAAAGGTAGGAATCTCCCAATCCTTCTGTTCATCTTCAATTTTCATTACCAGCATGTAATTCGAGCGCTTAGGATTCATAGCCCTAAACTTTGGAGTCTGAATTAAGCATCGTTGGGTTTAATTTGATTTTAGAGTTCTACGGTTCATATATGGTAGGGCTCGTATTTGGAAGTTTGAGATCGGATTAGGAACAAAAGGTAGTTTTAAATTTAGGGGTAGGGGAGGAANATACGAACTATTTTGATGGTTACGGGAAGGGTTAGGCTATTTTCGGGCCCCCTCCCGCCCGCCCGGCCGGAGA
TARGET=694,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01561 Ps_contig_mira-and-tgicl-ass_franssen_14913
SEQUENCE=GGTGTTTACCTTTAGGCCTGACTACATAGGTTCTTCCAAACTCAAATGCTCTTGCAGCAGATCTACCACCAACAGAACCGATTGATGGTGCAGCAAAGTTCATATTTGCCACTGAACAATACACCTATGGAACAGTCTTAAAGCTGAAGAGAGAAAGAGAGAGAGAGGGAGAGAAAGAGAGAGAGAGAATAGGGTTTTACAAACAAAGACGAGGATCTGAATTTCGAGATTCTAGAGACCACCCCCGCGTACTCTGTCGG
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3946
PRIMER_LEFT_PENALTY=0.243031
PRIMER_RIGHT_PENALTY=0.151533
PRIMER_LEFT_SEQUENCE=ATCTACCACCAACAGAACCGAT
PRIMER_RIGHT_SEQUENCE=CTCGAAATTCAGATCCTCGTCT
PRIMER_LEFT=60,22
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=59.757
PRIMER_RIGHT_TM=59.848
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.8000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.4508
PRIMER_LEFT_1_PENALTY=0.243031
PRIMER_RIGHT_1_PENALTY=0.207756
PRIMER_LEFT_1_SEQUENCE=ATCTACCACCAACAGAACCGAT
PRIMER_RIGHT_1_SEQUENCE=TCGAAATTCAGATCCTCGTCTT
PRIMER_LEFT_1=60,22
PRIMER_RIGHT_1=226,22
PRIMER_LEFT_1_TM=59.757
PRIMER_RIGHT_1_TM=60.208
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.8000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.4640
PRIMER_LEFT_2_PENALTY=0.312496
PRIMER_RIGHT_2_PENALTY=0.151533
PRIMER_LEFT_2_SEQUENCE=CAGCAGATCTACCACCAACAGA
PRIMER_RIGHT_2_SEQUENCE=CTCGAAATTCAGATCCTCGTCT
PRIMER_LEFT_2=54,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=59.848
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.4992
PRIMER_LEFT_3_PENALTY=0.347645
PRIMER_RIGHT_3_PENALTY=0.151533
PRIMER_LEFT_3_SEQUENCE=GCAAAGTTCATATTTGCCACTG
PRIMER_RIGHT_3_SEQUENCE=CTCGAAATTCAGATCCTCGTCT
PRIMER_LEFT_3=92,22
PRIMER_RIGHT_3=227,22
PRIMER_LEFT_3_TM=59.652
PRIMER_RIGHT_3_TM=59.848
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=0.4992
PRIMER_LEFT_4_PENALTY=0.347645
PRIMER_RIGHT_4_PENALTY=0.151533
PRIMER_LEFT_4_SEQUENCE=CAGCAAAGTTCATATTTGCCAC
PRIMER_RIGHT_4_SEQUENCE=CTCGAAATTCAGATCCTCGTCT
PRIMER_LEFT_4=90,22
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=59.652
PRIMER_RIGHT_4_TM=59.848
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_01562 Ps_contig_mira-and-tgicl-ass_franssen_14913
SEQUENCE=GGTGTTTACCTTTAGGCCTGACTACATAGGTTCTTCCAAACTCAAATGCTCTTGCAGCAGATCTACCACCAACAGAACCGATTGATGGTGCAGCAAAGTTCATATTTGCCACTGAACAATACACCTATGGAACAGTCTTAAAGCTGAAGAGAGAAAGAGAGAGAGAGGGAGAGAAAGAGAGAGAGAGAATAGGGTTTTACAAACAAAGACGAGGATCTGAATTTCGAGATTCTAGAGACCACCCCCGCGTACTCTGTCGG
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3946
PRIMER_LEFT_PENALTY=0.243031
PRIMER_RIGHT_PENALTY=0.151533
PRIMER_LEFT_SEQUENCE=ATCTACCACCAACAGAACCGAT
PRIMER_RIGHT_SEQUENCE=CTCGAAATTCAGATCCTCGTCT
PRIMER_LEFT=60,22
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=59.757
PRIMER_RIGHT_TM=59.848
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.8000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.4508
PRIMER_LEFT_1_PENALTY=0.243031
PRIMER_RIGHT_1_PENALTY=0.207756
PRIMER_LEFT_1_SEQUENCE=ATCTACCACCAACAGAACCGAT
PRIMER_RIGHT_1_SEQUENCE=TCGAAATTCAGATCCTCGTCTT
PRIMER_LEFT_1=60,22
PRIMER_RIGHT_1=226,22
PRIMER_LEFT_1_TM=59.757
PRIMER_RIGHT_1_TM=60.208
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.8000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.4640
PRIMER_LEFT_2_PENALTY=0.312496
PRIMER_RIGHT_2_PENALTY=0.151533
PRIMER_LEFT_2_SEQUENCE=CAGCAGATCTACCACCAACAGA
PRIMER_RIGHT_2_SEQUENCE=CTCGAAATTCAGATCCTCGTCT
PRIMER_LEFT_2=54,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=59.848
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.4992
PRIMER_LEFT_3_PENALTY=0.347645
PRIMER_RIGHT_3_PENALTY=0.151533
PRIMER_LEFT_3_SEQUENCE=GCAAAGTTCATATTTGCCACTG
PRIMER_RIGHT_3_SEQUENCE=CTCGAAATTCAGATCCTCGTCT
PRIMER_LEFT_3=92,22
PRIMER_RIGHT_3=227,22
PRIMER_LEFT_3_TM=59.652
PRIMER_RIGHT_3_TM=59.848
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=0.4992
PRIMER_LEFT_4_PENALTY=0.347645
PRIMER_RIGHT_4_PENALTY=0.151533
PRIMER_LEFT_4_SEQUENCE=CAGCAAAGTTCATATTTGCCAC
PRIMER_RIGHT_4_SEQUENCE=CTCGAAATTCAGATCCTCGTCT
PRIMER_LEFT_4=90,22
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=59.652
PRIMER_RIGHT_4_TM=59.848
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_01563 Ps_contig_mira-and-tgicl-ass_franssen_14914
SEQUENCE=GGTGTTTACCTTTAGGCCTGACTACATAGGTTCTTCCAAACTCAAATGCTCTTGCAGCAGATCTACCACCAACAGAACCGATTGATGGTGCAGCAAAGTTCATATTTGCCACTGAACAATACACCTATGGAACAGTCTTAAAGCTGAAGAGAGAAAGAGAGAGAGAGGGAGAGAAGAGAGAGAGAGAAGTAGGGTTTANAACAAGACGAGGATCTGTAATTTACGTAGTATTACTAGAGACCACCCCGCCGTACTCTGTCG
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3386
PRIMER_LEFT_PENALTY=0.243031
PRIMER_RIGHT_PENALTY=1.095566
PRIMER_LEFT_SEQUENCE=ATCTACCACCAACAGAACCGAT
PRIMER_RIGHT_SEQUENCE=GATCCTCGTCTTGTTNTAAACCC
PRIMER_LEFT=60,22
PRIMER_RIGHT=213,23
PRIMER_LEFT_TM=59.757
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.50
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=9.8000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.75
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=1.4081
PRIMER_LEFT_1_PENALTY=0.312496
PRIMER_RIGHT_1_PENALTY=1.095566
PRIMER_LEFT_1_SEQUENCE=CAGCAGATCTACCACCAACAGA
PRIMER_RIGHT_1_SEQUENCE=GATCCTCGTCTTGTTNTAAACCC
PRIMER_LEFT_1=54,22
PRIMER_RIGHT_1=213,23
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.50
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=1.4217
PRIMER_LEFT_2_PENALTY=0.243031
PRIMER_RIGHT_2_PENALTY=1.178627
PRIMER_LEFT_2_SEQUENCE=ATCTACCACCAACAGAACCGAT
PRIMER_RIGHT_2_SEQUENCE=TCCTCGTCTTGTTNTAAACCCT
PRIMER_LEFT_2=60,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=58.821
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.50
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.8000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=1.4432
PRIMER_LEFT_3_PENALTY=0.347645
PRIMER_RIGHT_3_PENALTY=1.095566
PRIMER_LEFT_3_SEQUENCE=CAGCAAAGTTCATATTTGCCAC
PRIMER_RIGHT_3_SEQUENCE=GATCCTCGTCTTGTTNTAAACCC
PRIMER_LEFT_3=90,22
PRIMER_RIGHT_3=213,23
PRIMER_LEFT_3_TM=59.652
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.50
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.75
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=1.4432
PRIMER_LEFT_4_PENALTY=0.347645
PRIMER_RIGHT_4_PENALTY=1.095566
PRIMER_LEFT_4_SEQUENCE=GCAAAGTTCATATTTGCCACTG
PRIMER_RIGHT_4_SEQUENCE=GATCCTCGTCTTGTTNTAAACCC
PRIMER_LEFT_4=92,22
PRIMER_RIGHT_4=213,23
PRIMER_LEFT_4_TM=59.652
PRIMER_RIGHT_4_TM=59.904
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.50
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.75
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=122
=
PRIMER_SEQUENCE_ID=SSR_01564 Ps_contig_mira-and-tgicl-ass_franssen_14914
SEQUENCE=GGTGTTTACCTTTAGGCCTGACTACATAGGTTCTTCCAAACTCAAATGCTCTTGCAGCAGATCTACCACCAACAGAACCGATTGATGGTGCAGCAAAGTTCATATTTGCCACTGAACAATACACCTATGGAACAGTCTTAAAGCTGAAGAGAGAAAGAGAGAGAGAGGGAGAGAAGAGAGAGAGAGAAGTAGGGTTTANAACAAGACGAGGATCTGTAATTTACGTAGTATTACTAGAGACCACCCCGCCGTACTCTGTCG
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3386
PRIMER_LEFT_PENALTY=0.243031
PRIMER_RIGHT_PENALTY=1.095566
PRIMER_LEFT_SEQUENCE=ATCTACCACCAACAGAACCGAT
PRIMER_RIGHT_SEQUENCE=GATCCTCGTCTTGTTNTAAACCC
PRIMER_LEFT=60,22
PRIMER_RIGHT=213,23
PRIMER_LEFT_TM=59.757
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.50
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=9.8000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.75
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=1.4081
PRIMER_LEFT_1_PENALTY=0.312496
PRIMER_RIGHT_1_PENALTY=1.095566
PRIMER_LEFT_1_SEQUENCE=CAGCAGATCTACCACCAACAGA
PRIMER_RIGHT_1_SEQUENCE=GATCCTCGTCTTGTTNTAAACCC
PRIMER_LEFT_1=54,22
PRIMER_RIGHT_1=213,23
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.50
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=1.4217
PRIMER_LEFT_2_PENALTY=0.243031
PRIMER_RIGHT_2_PENALTY=1.178627
PRIMER_LEFT_2_SEQUENCE=ATCTACCACCAACAGAACCGAT
PRIMER_RIGHT_2_SEQUENCE=TCCTCGTCTTGTTNTAAACCCT
PRIMER_LEFT_2=60,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=58.821
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.50
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.8000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=1.4432
PRIMER_LEFT_3_PENALTY=0.347645
PRIMER_RIGHT_3_PENALTY=1.095566
PRIMER_LEFT_3_SEQUENCE=CAGCAAAGTTCATATTTGCCAC
PRIMER_RIGHT_3_SEQUENCE=GATCCTCGTCTTGTTNTAAACCC
PRIMER_LEFT_3=90,22
PRIMER_RIGHT_3=213,23
PRIMER_LEFT_3_TM=59.652
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.50
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.75
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=1.4432
PRIMER_LEFT_4_PENALTY=0.347645
PRIMER_RIGHT_4_PENALTY=1.095566
PRIMER_LEFT_4_SEQUENCE=GCAAAGTTCATATTTGCCACTG
PRIMER_RIGHT_4_SEQUENCE=GATCCTCGTCTTGTTNTAAACCC
PRIMER_LEFT_4=92,22
PRIMER_RIGHT_4=213,23
PRIMER_LEFT_4_TM=59.652
PRIMER_RIGHT_4_TM=59.904
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.50
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.75
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=122
=
PRIMER_SEQUENCE_ID=SSR_01565 Ps_contig_mira-and-tgicl-ass_franssen_14951
SEQUENCE=TCGGGGTATGTCATAGTCTCATTCACAACAAACGCATATCACAAACACCTTCTCTCTCTCCCTCTCTCCCAAACCCTAGTATCTATCTCTCTTCACCGTTCGGTTTCATCATGAAAGGAGGAAAGTCCAAGGGTGAATCGAAGAAAGCTGAAACGAAGCTTGCGGTGAATAAGAAGGGGTGCTCCCCGCCAACAAAGGGGACGGGAAAAGAAAMCCAGCCGAAAGGGGAAAAGGGAAACCCAAAACGGRACCCCTAACAAGGACCGGAAGAAGA
TARGET=51,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01566 Ps_contig_mira-and-tgicl-ass_franssen_14952
SEQUENCE=GATTAGCAATTGAATTTCAATTTCCCTTTAAGGCCTTTAATATCCACCCGCATTTGTCCTAGCCTTAKGTCATAGTCTCATTCACAACAAACGCATATCACAAACACCTTCTCTCTCTCCCTCTCTCCCAAACCCTAGTATCTATCTCTCTTCACCGTTCGGTTTCATCATGAAAGGAGGAAAGTCCAAGGGTGATCGAAGAAAGCTGAAACGAAGCTTGCGGTGAATAAGAAGGGTGCTCCCGCCACAAAGGGACGGAAAGAAACCAGCCGAAAGGGAAAAGGGAAACCCGAAAACGGGAAACCCCCGTTAAAACCCCTAAAAAGGACCCCGGGAAAACGGAACGGTAAACCCCC
TARGET=110,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01567 Ps_contig_mira-and-tgicl-ass_franssen_14958
SEQUENCE=TCCCTTTAGATATACTGATTTATAAGTTGGTGAAATCTTATCTTCATGCAACACCATTTAAACGAGCAGCAGTGAAGGCTCTTTCAAAAGCATTGACAGAGGATCAGCTAGTGTATCTTCGAGCACAGTTCAGATTGTTGGAACCAAATAGAGACGGGCATGTCTCCCTCGACAACTTTAAAATGGCTCTTGCACGCCATGCAACTGAAGCCATGAGGGAATCAAGGGTTCTTGACATCATACATACGATGGAACCGCTTGCCTATAGAAAGATGGATTTCGAAGAATTTTGTGCAGCTGCAACGAGTACATATCAGCTGGAGGCACTTGATAGGTGGGAAGATATTGCCTGCACTGCATTTGAGCATTTCGAGAGTGAGGGAAACCGTGTGATATCGATCGAAGAATTAGCAAGAGAGCTAAATCTCGGTCCTTCGGCGTATTCAGTTCTGAGAGATTGGATTCGAAATACTGACGGAAAGCTTAGTTTACTTGGATATACAAAATTTTTGCATGGAGTGACTCTGCGGAGTTCTGTACCAAGACCTCGTCAGTCTTGAGCTCTTTATTTATTTAGCACATAAAGATGGGAGAAAAAGATTTATAGGTTCATTTATAGATTTATAAGGATTATTTTTTACTTTGGATTTCTCTTTCGGGTGGTGAAAAGTGGAATGTGTATAAATGAATGATGTGTTGGAAATATGAGAAATGTTGAGTAAATTTCATGTTACAGTACCCT
TARGET=565,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5447
PRIMER_LEFT_PENALTY=0.203971
PRIMER_RIGHT_PENALTY=0.340760
PRIMER_LEFT_SEQUENCE=ATTGGATTCGAAATACTGACGG
PRIMER_RIGHT_SEQUENCE=ACCCGAAAGAGAAATCCAAAGT
PRIMER_LEFT=456,22
PRIMER_RIGHT=660,22
PRIMER_LEFT_TM=60.204
PRIMER_RIGHT_TM=60.341
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.5998
PRIMER_LEFT_1_PENALTY=0.203971
PRIMER_RIGHT_1_PENALTY=0.395857
PRIMER_LEFT_1_SEQUENCE=ATTGGATTCGAAATACTGACGG
PRIMER_RIGHT_1_SEQUENCE=CCCGAAAGAGAAATCCAAAGTA
PRIMER_LEFT_1=456,22
PRIMER_RIGHT_1=659,22
PRIMER_LEFT_1_TM=60.204
PRIMER_RIGHT_1_TM=59.604
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.6529
PRIMER_LEFT_2_PENALTY=0.312177
PRIMER_RIGHT_2_PENALTY=0.340760
PRIMER_LEFT_2_SEQUENCE=GGATTCGAAATACTGACGGAAA
PRIMER_RIGHT_2_SEQUENCE=ACCCGAAAGAGAAATCCAAAGT
PRIMER_LEFT_2=459,22
PRIMER_RIGHT_2=660,22
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=60.341
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.7056
PRIMER_LEFT_3_PENALTY=0.364827
PRIMER_RIGHT_3_PENALTY=0.340760
PRIMER_LEFT_3_SEQUENCE=ATTCGAAATACTGACGGAAAGC
PRIMER_RIGHT_3_SEQUENCE=ACCCGAAAGAGAAATCCAAAGT
PRIMER_LEFT_3=461,22
PRIMER_RIGHT_3=660,22
PRIMER_LEFT_3_TM=59.635
PRIMER_RIGHT_3_TM=60.341
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=0.7080
PRIMER_LEFT_4_PENALTY=0.312177
PRIMER_RIGHT_4_PENALTY=0.395857
PRIMER_LEFT_4_SEQUENCE=GGATTCGAAATACTGACGGAAA
PRIMER_RIGHT_4_SEQUENCE=CCCGAAAGAGAAATCCAAAGTA
PRIMER_LEFT_4=459,22
PRIMER_RIGHT_4=659,22
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=59.604
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=201
=
PRIMER_SEQUENCE_ID=SSR_01568 Ps_contig_mira-and-tgicl-ass_franssen_14959
SEQUENCE=GAAGAAAACAGAAGAWCGAGKBATTCGTCAATTTTCTGTTTGTCTGAAACACATGTGCGTCTATATATATACTCTCACATTTTCTGTTAACAAACCAATAACTTGTCGCTTCGTCTTCTTCTTCTACGTTCTCACTCCAAACCACCACWAWTTTGAGATGGAAAACCCTTCCTCTTCACCTCAGAATCTGTAAATGAAGGTCCACCCCGACAAGCTGTGTGATCGTAGGTTTCAGATGCCATTCCTTGA
TARGET=62,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01569 Ps_contig_mira-and-tgicl-ass_franssen_14959
SEQUENCE=GAAGAAAACAGAAGAWCGAGKBATTCGTCAATTTTCTGTTTGTCTGAAACACATGTGCGTCTATATATATACTCTCACATTTTCTGTTAACAAACCAATAACTTGTCGCTTCGTCTTCTTCTTCTACGTTCTCACTCCAAACCACCACWAWTTTGAGATGGAAAACCCTTCCTCTTCACCTCAGAATCTGTAAATGAAGGTCCACCCCGACAAGCTGTGTGATCGTAGGTTTCAGATGCCATTCCTTGA
TARGET=114,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01570 Ps_contig_mira-and-tgicl-ass_franssen_14960
SEQUENCE=CTCAACAGTCACTTGGGTTTTACCATCAGGCCTAAGCCATGGACATGTTTTGTKCTTTCTAACTTCAGTGAGCTTTGCACCAACTTTAGTAGCAAGCACATGAGTGAGTGGCATTAGCTCAGGTGTTTCGTCTGTGGCATAACCAAACATGTGGCCTTGGTCACCAGCACCAATATCCTCTGGTTTTTTGGTCAAGTGACCATGAACTCCTTGAGCAATATCAGGACTCTGTTGCTCAATGTTGACAAGAACTTTGCAGTTGTCAGCGTCAAGACCGACATCAGCCGATACAAATCCAATTCCTCTGCAAGTGTCTCGAACTATTTTCTCATAGTTCACATTAGCCTGGGTTGTGATCTCGCCAAAGACCATAACCATGTTAGTCTTTGTACATGTCTCACAGGCAACCTTACTTTCCGGGTCTTGCTCCAAACACGCATCAAGGATGGCATCTGAAACCTGATCACACAGCTTGTCGGGGTGACCTTCATTTACAGATTCTGAGGTGAAGAGGAAGGTTTCCATCTCAAATTGTGGTGGTTGGAGTGAGAACGTAGAAGAAGAAGACGAAGCGACAAGTTCCT
TARGET=556,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01571 Ps_contig_mira-and-tgicl-ass_franssen_14985
SEQUENCE=AAGATTTTAAGTTACTCATATATTTTCCAGAGTTAATGTACATGTACATAGTTACATTGCATGTGTTCAAGAAAAAGAAGTTACATTACATTACATGTTACAGCCATTATTGTTGAAGATAGAGATGCATCCAACAAAAGAGAATAAGACCATTATTGTTGCATAACCTTTCGCATTGACGCCAGTTAGAACTATATAAATTACACATTCGGACTAAATGTGAGTATGGCTATCAAGTTATGTCACATTATTTCTTCAAACTCACCATTTATCCAAACATGCACTAAATAACTATTAAAATGGAGCTGTAAGCTCGGCCGCCGATTCTTCCTCTTTCCCCTTCATTTCTCCACCGTTAGCCACCACCTCCTCCGCTTCCCACATGAAGTGTGCATAGGATCCTAACACGGTACTATCATCAGGAGCAGCATGAATGGCTTGATCGAAGTAAGATTGAGCTCTTTCTTCATCTCTATTCATTTCCCATATCAACTTTCCGTACAGAGAAAGCAACTCGCCATCTTCAGGATTCGCTAGAATTGCTCTTCCGTAATATTCCTCTGCTCTCACCAAATTTTTTTCTACCTCGTGAAGAAATTTGCCGTAGTTTCTAAGCAAAAGCGCGTCCGTTGGATTTGACTTCAACATTTCCTCATAGTACGCGCCGATTTTAATCCTTTCACCGCCGTTTCCGCCGCTGGCGACTATCACATCACCGTGATCGCATCCGCCGCCGCCGAGTCCGGCCTCCTCCAAAGGAAAATACTGTGATCCGGATCGATTCAGGTTCCTAGAAAATCCGAAATTCTCGTTTGGTGATCGTATTGCAGCGCTTTCCGATAACTCTCTGCTGACTTCCCGTTTGAATGCGTGGGTCGGTGGAGTAGGAAATGCAGGTAGAAAGCSTYGGAGATTGAACGGTTGGTTGGTTTTT
TARGET=81,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01572 Ps_contig_mira-and-tgicl-ass_franssen_14985
SEQUENCE=AAGATTTTAAGTTACTCATATATTTTCCAGAGTTAATGTACATGTACATAGTTACATTGCATGTGTTCAAGAAAAAGAAGTTACATTACATTACATGTTACAGCCATTATTGTTGAAGATAGAGATGCATCCAACAAAAGAGAATAAGACCATTATTGTTGCATAACCTTTCGCATTGACGCCAGTTAGAACTATATAAATTACACATTCGGACTAAATGTGAGTATGGCTATCAAGTTATGTCACATTATTTCTTCAAACTCACCATTTATCCAAACATGCACTAAATAACTATTAAAATGGAGCTGTAAGCTCGGCCGCCGATTCTTCCTCTTTCCCCTTCATTTCTCCACCGTTAGCCACCACCTCCTCCGCTTCCCACATGAAGTGTGCATAGGATCCTAACACGGTACTATCATCAGGAGCAGCATGAATGGCTTGATCGAAGTAAGATTGAGCTCTTTCTTCATCTCTATTCATTTCCCATATCAACTTTCCGTACAGAGAAAGCAACTCGCCATCTTCAGGATTCGCTAGAATTGCTCTTCCGTAATATTCCTCTGCTCTCACCAAATTTTTTTCTACCTCGTGAAGAAATTTGCCGTAGTTTCTAAGCAAAAGCGCGTCCGTTGGATTTGACTTCAACATTTCCTCATAGTACGCGCCGATTTTAATCCTTTCACCGCCGTTTCCGCCGCTGGCGACTATCACATCACCGTGATCGCATCCGCCGCCGCCGAGTCCGGCCTCCTCCAAAGGAAAATACTGTGATCCGGATCGATTCAGGTTCCTAGAAAATCCGAAATTCTCGTTTGGTGATCGTATTGCAGCGCTTTCCGATAACTCTCTGCTGACTTCCCGTTTGAATGCGTGGGTCGGTGGAGTAGGAAATGCAGGTAGAAAGCSTYGGAGATTGAACGGTTGGTTGGTTTTT
TARGET=728,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01573 Ps_contig_mira-and-tgicl-ass_franssen_14985
SEQUENCE=AAGATTTTAAGTTACTCATATATTTTCCAGAGTTAATGTACATGTACATAGTTACATTGCATGTGTTCAAGAAAAAGAAGTTACATTACATTACATGTTACAGCCATTATTGTTGAAGATAGAGATGCATCCAACAAAAGAGAATAAGACCATTATTGTTGCATAACCTTTCGCATTGACGCCAGTTAGAACTATATAAATTACACATTCGGACTAAATGTGAGTATGGCTATCAAGTTATGTCACATTATTTCTTCAAACTCACCATTTATCCAAACATGCACTAAATAACTATTAAAATGGAGCTGTAAGCTCGGCCGCCGATTCTTCCTCTTTCCCCTTCATTTCTCCACCGTTAGCCACCACCTCCTCCGCTTCCCACATGAAGTGTGCATAGGATCCTAACACGGTACTATCATCAGGAGCAGCATGAATGGCTTGATCGAAGTAAGATTGAGCTCTTTCTTCATCTCTATTCATTTCCCATATCAACTTTCCGTACAGAGAAAGCAACTCGCCATCTTCAGGATTCGCTAGAATTGCTCTTCCGTAATATTCCTCTGCTCTCACCAAATTTTTTTCTACCTCGTGAAGAAATTTGCCGTAGTTTCTAAGCAAAAGCGCGTCCGTTGGATTTGACTTCAACATTTCCTCATAGTACGCGCCGATTTTAATCCTTTCACCGCCGTTTCCGCCGCTGGCGACTATCACATCACCGTGATCGCATCCGCCGCCGCCGAGTCCGGCCTCCTCCAAAGGAAAATACTGTGATCCGGATCGATTCAGGTTCCTAGAAAATCCGAAATTCTCGTTTGGTGATCGTATTGCAGCGCTTTCCGATAACTCTCTGCTGACTTCCCGTTTGAATGCGTGGGTCGGTGGAGTAGGAAATGCAGGTAGAAAGCSTYGGAGATTGAACGGTTGGTTGGTTTTT
TARGET=920,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01574 Ps_contig_mira-and-tgicl-ass_franssen_15018
SEQUENCE=GACTTCCTTTTTCTCTTTTTTCTCGTCTCCGTTACAACAGTTCCTTCCTTCTAATCACTTCCATCCTAAATTCTCTCCTTTCTCCATAACTAACCAATCCCTTCCTTAACTATTTCTTCAACATTTTCCGTTACCTCTTATATTTCACAAACAACGTCTTATTATGGAACTTGTAACCGCAACTCATCTTTTACTCACTGCAACGCTTGGACTTCTCATTTTCTTCATCTTCGCAAAACTTGTTTCCATAGCAACCACCGACAATAGCACAACGACAAAACATCGTGTTCAGGAACAACCGGTTGGTCCGGTTCTTCAACGGTTGGCGGTTCAGTCACCTCAAACCAAGAGCCGAGTTGGTTTTGTTAAACCGGTTCAAGTTACAACAACAACAACGACAACAACTAGTTTAGAAACCGACGACGCTATTGTCAATTCCGATTCTGGAGTTGATGTCGAGTTGCCACCGACAAACTCAAACGTTGACGAAACTGAACACGATGACATAGGAGTCGCTAAGGTTGAATCTGATTCCGAGGTTGTTGAATGTTTAGAAGAAACGGTTGTGAAAGGTGGCGATTCTACTGAAGAGAAGAGCATTGAGTATGTGGAAGAAGCCACCGGAGAAAATTCCGGTGAGAAGTTAGTTTCTGATGTGGTGAGCGAAGGAGAGGAAAAGGAAGATGATGAAGATTGGGAATGGGAAGGGATTGAGAGGAGTGAGGTGGAGAAGACGTTTATGGCGGCTACGGAATTTGTTGCTGAGAAAGGGTATATTGGTAAATGCGGTGATGATTTGGAGATGGTGTTGTATGGACTTGAGAGAGTTGCTATTGAAGGACCTTGCCGTGAACCTCAACCAATGCCGCTTAAACTCTCCGCACGTGCTAAATGGAATGCTTGGCAAAAGCTGGGGAACATGAGTCCAGACGTTGCAATGGAGCAATATATCGGCCTTGTTTCCGATAAATTTCCTGGATGGATGAAAAATACTTCTACTGGAATGAGTGAAGGTGGACCCTCGGGATCAGAAGTTTCTGAGTCTGCTGCTCTGGGTTTGAGCTCAGCTTCGTCTCATCAACAAACAAGCTTAGCAGACAGGAAACTTGTTCAAGAATCTAAGTCTGGTGCCCAGGAGTTTATCCCATATGCAGAGTCAGATTCCGAGAACAATGTTAAAAAATGATGGCATACCGCACCATGGGACATTTTCCTACATTCATTTCAGCTATAAGAAGACTGATCCTAAGATTATAAGTTCTTTTTCAAATTATGCGAGATTCTCGAGCTGTTTGCTTTCTCCTTTATTACTTAAATTATGTGTTGATTGTTGATGTGTTATTCGATATCGATCCGATGAAATGTTAATAGATATTTTCTGTAAATAAATAAATGCATGTAATTATGATCTTATCTTCGGTAGAAGGTTCAATAATTCCTATTTGTTATATCAAATTTACAATTCATATCYC
TARGET=383,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01575 Ps_contig_mira-and-tgicl-ass_franssen_15018
SEQUENCE=GACTTCCTTTTTCTCTTTTTTCTCGTCTCCGTTACAACAGTTCCTTCCTTCTAATCACTTCCATCCTAAATTCTCTCCTTTCTCCATAACTAACCAATCCCTTCCTTAACTATTTCTTCAACATTTTCCGTTACCTCTTATATTTCACAAACAACGTCTTATTATGGAACTTGTAACCGCAACTCATCTTTTACTCACTGCAACGCTTGGACTTCTCATTTTCTTCATCTTCGCAAAACTTGTTTCCATAGCAACCACCGACAATAGCACAACGACAAAACATCGTGTTCAGGAACAACCGGTTGGTCCGGTTCTTCAACGGTTGGCGGTTCAGTCACCTCAAACCAAGAGCCGAGTTGGTTTTGTTAAACCGGTTCAAGTTACAACAACAACAACGACAACAACTAGTTTAGAAACCGACGACGCTATTGTCAATTCCGATTCTGGAGTTGATGTCGAGTTGCCACCGACAAACTCAAACGTTGACGAAACTGAACACGATGACATAGGAGTCGCTAAGGTTGAATCTGATTCCGAGGTTGTTGAATGTTTAGAAGAAACGGTTGTGAAAGGTGGCGATTCTACTGAAGAGAAGAGCATTGAGTATGTGGAAGAAGCCACCGGAGAAAATTCCGGTGAGAAGTTAGTTTCTGATGTGGTGAGCGAAGGAGAGGAAAAGGAAGATGATGAAGATTGGGAATGGGAAGGGATTGAGAGGAGTGAGGTGGAGAAGACGTTTATGGCGGCTACGGAATTTGTTGCTGAGAAAGGGTATATTGGTAAATGCGGTGATGATTTGGAGATGGTGTTGTATGGACTTGAGAGAGTTGCTATTGAAGGACCTTGCCGTGAACCTCAACCAATGCCGCTTAAACTCTCCGCACGTGCTAAATGGAATGCTTGGCAAAAGCTGGGGAACATGAGTCCAGACGTTGCAATGGAGCAATATATCGGCCTTGTTTCCGATAAATTTCCTGGATGGATGAAAAATACTTCTACTGGAATGAGTGAAGGTGGACCCTCGGGATCAGAAGTTTCTGAGTCTGCTGCTCTGGGTTTGAGCTCAGCTTCGTCTCATCAACAAACAAGCTTAGCAGACAGGAAACTTGTTCAAGAATCTAAGTCTGGTGCCCAGGAGTTTATCCCATATGCAGAGTCAGATTCCGAGAACAATGTTAAAAAATGATGGCATACCGCACCATGGGACATTTTCCTACATTCATTTCAGCTATAAGAAGACTGATCCTAAGATTATAAGTTCTTTTTCAAATTATGCGAGATTCTCGAGCTGTTTGCTTTCTCCTTTATTACTTAAATTATGTGTTGATTGTTGATGTGTTATTCGATATCGATCCGATGAAATGTTAATAGATATTTTCTGTAAATAAATAAATGCATGTAATTATGATCTTATCTTCGGTAGAAGGTTCAATAATTCCTATTTGTTATATCAAATTTACAATTCATATCYC
TARGET=1382,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01576 Ps_contig_mira-and-tgicl-ass_franssen_15025
SEQUENCE=TTTATGAGAGAAACAAAACTCTTTACATTAGCTCATCGACAACATTATACAAATGCACAAGAATTTACTTCAACCAAACAAATGGAACATAGACATGACTCAAGAAAAGCGAGTAGCAGACAAGGATGAGTCACAATCTATCCATCTATATTTCCGCGACAGTCATTCTTCAGGATTGCAAGTATTTTCAAGGTATCATCAATGCAGTTCAACAGAGGATGCCATAGTATTTTGAGTGATACCTAAAATTCTGTGTCAAAACATGAAACCATGATCTTTGAAACCATGGACCTAGCAAAAGGGATGTAGCTCAAACTATACCAAACTAACGCCCCAAACTCCAAGATAATCGCGAGAAGTGTAAGTAACTTGTTATGAATATAAAGTGCACAAAACAAGGCAATTATCATACTTGCAATGTATATGGCAGTTGCATAAATACGAACAGGATCAAGCATCATCGATACTTGCCTTTTAGGACCTATGAGGAATGCTGTGCTTCCAAGTGAAAGCAAATTGCCAAGAGTGAAAGTTATCCCAAACTTGATTGGTTTGAGGAAAACAAGCATAGACAACAGTGTACAGGTTACTCCAGTAGCAAAGCATATGGCAAAACCATAAAGTCTCTGTTTGGTAGTCAAAGTGCAGTTACGGTTGAAATCGTCCATGAAAGCGAATGAGTTTTCGTTATCCAATGCTGCAGCTTGTTGCTCTTCGTCTTCGACTTCCATTCCTACCATCATTTTCACTTTCTCAAAGGCTTTATTCATTTTTTCCATTGTTTTTTCTTCAGATTGTCGGGAAAATGGAAGAGGAAAATTTAGAGAAAGAGAGAGAAAGATCTGGTGTTTGTGGGAAGAAAGAAAGAGGAAGTAAGGGATGAAGTTGGTTAGATTTTCAGAAATTGTTTTGAGGAATTCGATTGAGATGTAACAACGACTTCACTACAGTGAAACCAAACTCAACGATCCCCGGTA
TARGET=857,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4351
PRIMER_LEFT_PENALTY=0.114470
PRIMER_RIGHT_PENALTY=0.320679
PRIMER_LEFT_SEQUENCE=CTCTTCGTCTTCGACTTCCATT
PRIMER_RIGHT_SEQUENCE=TCGTTGAGTTTGGTTTCACTGT
PRIMER_LEFT=710,22
PRIMER_RIGHT=967,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=59.679
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.4510
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.320679
PRIMER_LEFT_1_SEQUENCE=CGTCTTCGACTTCCATTCCTAC
PRIMER_RIGHT_1_SEQUENCE=TCGTTGAGTTTGGTTTCACTGT
PRIMER_LEFT_1=715,22
PRIMER_RIGHT_1=967,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.679
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.5284
PRIMER_LEFT_2_PENALTY=0.207756
PRIMER_RIGHT_2_PENALTY=0.320679
PRIMER_LEFT_2_SEQUENCE=CGACTTCCATTCCTACCATCAT
PRIMER_RIGHT_2_SEQUENCE=TCGTTGAGTTTGGTTTCACTGT
PRIMER_LEFT_2=721,22
PRIMER_RIGHT_2=967,22
PRIMER_LEFT_2_TM=60.208
PRIMER_RIGHT_2_TM=59.679
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.5000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.6403
PRIMER_LEFT_3_PENALTY=0.319597
PRIMER_RIGHT_3_PENALTY=0.320679
PRIMER_LEFT_3_SEQUENCE=TTCGACTTCCATTCCTACCATC
PRIMER_RIGHT_3_SEQUENCE=TCGTTGAGTTTGGTTTCACTGT
PRIMER_LEFT_3=719,22
PRIMER_RIGHT_3=967,22
PRIMER_LEFT_3_TM=60.320
PRIMER_RIGHT_3_TM=59.679
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.6855
PRIMER_LEFT_4_PENALTY=0.364827
PRIMER_RIGHT_4_PENALTY=0.320679
PRIMER_LEFT_4_SEQUENCE=AGCGAATGAGTTTTCGTTATCC
PRIMER_RIGHT_4_SEQUENCE=TCGTTGAGTTTGGTTTCACTGT
PRIMER_LEFT_4=671,22
PRIMER_RIGHT_4=967,22
PRIMER_LEFT_4_TM=59.635
PRIMER_RIGHT_4_TM=59.679
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_01577 Ps_contig_mira-and-tgicl-ass_franssen_15031
SEQUENCE=AAAHTTCAAACTGAATTTGATTTTATTTTTTAAATTGGAATCCTTTTACTATAAAATCGATTCAACTCACACCACTCTTGGTACAAACTCCACCTAGAATAAAATTTAAACAAAAATTGTATCTAAAATTATGATGTATCTAATGTTAATTTTTAACTAAATACATTAACAATTGGTACATACTTGTCCTAGAATTCCATATGCTGAAGAGTTGTGATGCAAAACATGGCGATGTCTGTCTACTCTTCAATTGTTAGTCCGTCCATGGAAGCTGCTTCAAGAGATGTATCCATTCGAGCATTTTGAAAGCGTCTTGAAAACTGTTTCAACAACTTGATTAACTCATATGTTCTAAAACTTTGACCGGAGTAGAGGCAAATGTCAAGTGGAGTTAAACCATCCTTGTTTTTTAATGTCTTATCAGCACCTTTAATCAATAGAAGCCTTACTACATCATTTCTCTGAGTTTGTACAGCAAGATGTAATGGTGTCCAGCCATCATTGTCCGGAAGATTTATATCCACATTATACAACAAAAGCACTTTAATTGTTGGGACAGAAGCTGTTTGTACAGCATAATGCATCAAGGTAGCCCCATCATTATCTTGTACAAAGGGATTGGCTGAGTTTCGCAGGAGAAGATTAGTTATGGCACGCTTTCTTCCAATTATCGATTTTGAAAGAGCAGTCAAACCATCCTTATCCATAGCATTAATATCAACCTCGTGCTTCAACAAAGTTTGTAAGAGGAAAGATTCTCCGCTCGCAGCAAAAGTATGCAGAGGAAGCCATTTATCCGAAGTTGCAACTGACAAGTCTGGCTTAATCTTATTCAACAAAGCCTTCTCCTCCTTTGTAAACAACTTAGGACGACCTTCAGTTTTCTTTAACTAAGCAAGATCATAACTTTCACTATTCAAAACTCATTGACATCATCCAAACCTCTTCCATAAACAGCCCACCTAGGATTCTTAGGCTCAACGCGTTCTTCACCAGACCCATCACTCAATTCATCTTCCAACTCTTCCTCTTTCTCAGCAAAACTAAAACTTGGAGGCTTATCAAGAACCACAGGTTTCAACTTAGCCAACATAGCATCTCTATAAGCTTTGTAATCATCCAAATCCATATGCCTCAAAGGAACCAACCCTCGTTTCTTCTTCAAAACCTTACTATTGAACTCAATTTCCTGAGCTATCTTACGTCTCTCTTTCTTCGTTATGTTTGCCTCTGCTTGAACATCTCTCCACTTGTCAGGAACCAAATTCTCCGACTCAACTGTTACTGTTTTTTTCTTCTTTTTCTTTGAAGTGGGTTTTGGTTTTTTGTTGTCTACGAGGTTGTCGGAAGGAGTAGGAAATATGGGGTCTTCGAAAATGCCTTCCTCGAAGACGAGACAATCTCCGATTACATGTTCTTCGTAGTTGTCGTTTTGGTAGGATGAGGAGAGAGAATTGTGGAGGTTCCAGTTTGTGGGGAGTTTGAGAGATTGAAATTTGCGGGGTGTGGCAGAAGAAGGGGGGAATGGGAATGGAGAGAAGAAAAGTGTGTTGGATTGAGGATTATGCACAAGTGTGGAGAAATTGAACATTGTTGTTGTTGATGTTGCTTYTRCGTACTCTG
TARGET=1591,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01578 Ps_contig_mira-and-tgicl-ass_franssen_15040
SEQUENCE=TAGATGAAAACAATTGAATGTTGCCAACTCAGAAATAAAGGGCGACTGATCCAGCAACAAGRACGGCAGCCGATCGCGACTTAGTAGCACCTCCTGTTTCCATCATTCTGTTCATAGTTTTTTCATATTCTCCTTCAATCTTCTTCTTCATCTCTCTTAATCCCACCTTCGTCATCCATGAGCCTTCGTTCTTCAATTCTGCATCAGAATTCTACAATCGAAAATCCGCCGCAGCCAAGATTCTTCAACTGAACCTTCATTCTTCTTCTTCATCATCCCGCAATAATTTCTCTTCTCCATCACTCTGCAGCTTCTCCTTCATCGATCTACAACGATTTCGACCCCTCAATTCGCAGCACAGCYTTTTTACCGGTGAGTCATTCGTTCTTGCTTCAATTCCCATTGTCGCGTTCTGTTTCCTCTTTCTATTTGCGCACGTAGCAGATTGAGTCGAGCTCACGAATATTCGAACCGTGATGGATGATGAATGATAAGCGAAAAACGAATGCGAAGGACAAGAGGAAAGATTGAACGCGAATCAAATGCCATTGATGGACGGGGGTGATCCGCGGATGTGACATCGAATGCGATGCGAAGATACGCGACGGTGGAGTCGTGAAAACGTGACGCCAGCGTACACCGTAACGACGCGCGTTGCTGCGAAGAAAGAGTGATGGAGCCTGCCGCCAAAACACGATGATGGATCGAAGCCAAGACGGACGATTTCCTCATGATTCTTCGAGCAAAAGAACGCATATGGTGAAGCGGAATTGGAATGTGCTTACTGCTGCCGTTTGGTCGTTTGACCTATTTTCGTTC
TARGET=260,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01579 Ps_contig_mira-and-tgicl-ass_franssen_15047
SEQUENCE=TATCGNACTCGCTNNTNNTTCACWCCMTYCNTAACATGCCATCTATTGTTATTCTTTTAATCTTTTCGANGGCCTTGCCTTGGAAAGATGCGCATCAACATCTCTAGTGCATTGGCTAATGTAGTCCATCCATCCATTTGGAGGATGAATATCCTCGTTGATTTTCTCAAATTCAACGGTCCATTTAGCAACAGCAGTGCCATCTTCAGCCTTATCAATTACTTGGAAGGGTGAGACTTAAAGGACTTATAGTGCTTATCAATATCCTACCCAAACCCTAAAAATTACGGT
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01580 Ps_contig_mira-and-tgicl-ass_franssen_15053
SEQUENCE=AGGGGCGTATCTCTTATCCTATCTCATCTCAGTCCTGTTCTGTTTCTCCCTCCACGGTGGCAGAGGCTGTCCTCGGTGGCAGAGGCTCTCCAAAATCAAGTCAAACACCAAAATTCAAACCGAAATCACACAATGAGTCTGAATCCACCAACGGATTTCCTTGATTTTTCATGGACTATGCAAATCGAAGAGAGAGCTTCAAGAACCCTAATGCGAAAATTCGAAATTAAATAGCATCAGAGGGGGATCGAGCCACCTCACATTGGGGATTTGATTCCCCTCGTGAAGGACTACGCAATGGTAACTCAAAATTTCAAAAAGAAAGAAGATGATTGAAGTCTCACCRAAGCACACACCGACGTCAAATACAACTTGAAGTTTTCTTCTTATCCTCTTTCTCTTATTCTTCTTCTTCTTCTCTGTGATGTGATTCTGCTTCTTCTGTGCGTTGCAAATTGCGAATCGAGAATTGTTGTGAGAGTTTGGTGATGAATGAGTGTGGTTGATCTTTGAGGATTGAAGTGATTGTTTGTGGAAGCTTGGTGATGAATGAGTGGAGAAGATGATGATGAATTAAGATTGATGATGAGGTTACTGAAATTTGGTTTGTGGAGGGTGATTTGAGGAAATCGAAAATGTGAAAGAAATGAGGATTGAAGGTGTTGAATTGTGAGAATACTAAAAAAAGTGAGAGTTGAAGCCTCTAGAAAAATAAGTAATAGTACTTAGTAAGTTTTTCTCA
TARGET=404,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01581 Ps_contig_mira-and-tgicl-ass_franssen_15059
SEQUENCE=GAAGGTAGCACATCTGTGTCAGTATGGTGGTGGAGGGTACATCCCAGCATTTGGAGGATAAGTTGCAGGTGGAGGGTAGGAAGGTGGTGGATAAGCAGAGTGGGGAGGAGGGGGGTATGGGGAGGAGGAGGGTGGTGGATAAGGAGATGGCATGGATGGATAGGTTGAGGGTGGTCGATAGTGAACTGAATTATGCGAAGCAGCAGGAGGAGGAGGAGCAGGATAAGGTTGTTGTGGATAAGCAAGAGCAGGAGCAGGTGTTGGATGTTGATAATTAGAGTATGATGGTTGGTGTGGGTAGTAGGAAGGAGGGACTGTGGGTGCTGCTCCATAGGAAATAGGAGTATGGGTTGAGGAGGAGCCTTGTTTCTGGTGATTTAGTTTGGCGTTCGCATAATGCAATATAAGTTTAATTTCGCCTGCGTGTCTGCCAGATTTACTTTGAAGAGGCCAACAAGAGTCATCAAAACCTTGAGACAGAACCTTGTTCAATTGAACCTTTCCGGTGCCGATAAAATCATCCAATTTGAGAGTATTGCTGTTCCAAACGACAACAGTGAGTTCGCGAAGACCTTCAATCAGCGGCACAACGAACTTCTCTTGAAACACCGGATATTTTCCACCGTCTGTGCAGGTTCTTGTTCGGAATTTGGTGGACGCGTATTCAAGACAGACGTAGGGATTCTTGTCTTGAAATCCATTCAGTGTCCTTCAATTTCGAGCAAGAAACCACCGTAACCTCAAGAGGTTGACCCTGAATCCCCGCTACCGTTCCAGAAGACATTGTTTTTTGATCGGAATATGCTCGCTGATTTAGATCCACAACGTTACTTATACTAAAATTTACTTTTCAATCAAACTGTGGGCATTTATTTTCATACTTATCTCTTTACTTTACGCGTATATTTCCTTTCTTCTCTATTTTACCAAATCAAAACAATAAAACTTCATCATCTTTTACGACTTGACCAAAATAAAATACCAGAAAAACAAAACATGTTGTAACGTTTAAGATTTTTAGATTTTTTTAACAAAACAAACTTATTCATTAAGGAAATGCCCGCACTTGGAATTTTGGCCTCC
TARGET=205,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1145
PRIMER_LEFT_PENALTY=0.114470
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=TAGGTTGAGGGTGGTCGATAGT
PRIMER_RIGHT_SEQUENCE=TATCAACATCCAACACCTGCTC
PRIMER_LEFT=160,22
PRIMER_RIGHT=272,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=113
PRIMER_PAIR_PENALTY_1=0.1594
PRIMER_LEFT_1_PENALTY=0.114470
PRIMER_RIGHT_1_PENALTY=0.044904
PRIMER_LEFT_1_SEQUENCE=TAGGTTGAGGGTGGTCGATAGT
PRIMER_RIGHT_1_SEQUENCE=TCCTCCTCAACCCATACTCCTA
PRIMER_LEFT_1=160,22
PRIMER_RIGHT_1=359,22
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.3000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.1925
PRIMER_LEFT_2_PENALTY=0.114470
PRIMER_RIGHT_2_PENALTY=0.078029
PRIMER_LEFT_2_SEQUENCE=TAGGTTGAGGGTGGTCGATAGT
PRIMER_RIGHT_2_SEQUENCE=CTCTTGTTGGCCTCTTCAAAGT
PRIMER_LEFT_2=160,22
PRIMER_RIGHT_2=459,22
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=59.922
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.3078
PRIMER_LEFT_3_PENALTY=0.114470
PRIMER_RIGHT_3_PENALTY=0.193337
PRIMER_LEFT_3_SEQUENCE=TAGGTTGAGGGTGGTCGATAGT
PRIMER_RIGHT_3_SEQUENCE=CAAAGTAAATCTGGCAGACACG
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=443,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=59.807
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.3000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.3169
PRIMER_LEFT_4_PENALTY=0.271999
PRIMER_RIGHT_4_PENALTY=0.044904
PRIMER_LEFT_4_SEQUENCE=GTGAACTGAATTATGCGAAGCA
PRIMER_RIGHT_4_SEQUENCE=TCCTCCTCAACCCATACTCCTA
PRIMER_LEFT_4=180,22
PRIMER_RIGHT_4=359,22
PRIMER_LEFT_4_TM=60.272
PRIMER_RIGHT_4_TM=59.955
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=180
=
PRIMER_SEQUENCE_ID=SSR_01582 Ps_contig_mira-and-tgicl-ass_franssen_15064
SEQUENCE=CGCGGAAAAGATAAGAGACCGGGTTCTAAGCGGCACCGCAAAGTTGTGGCCGGAAATAGAGACATTGAAGCAAAATTTAAAAGTTTTGTTTTGGGTGATTATATCATTAAATTTTTGTTGTTTGTTTTTTCTTTTGATATTCTATTCTACCTACGAAAATCATTCATTGCTGAACTGAGGGAAAATGTGGAAGTAGAAGATGAATGTTGTATTGTTTCCACACGGACACTTCTTTATTTCTTTATTACTTTGTGCTAATTTGTTAAACGTTTGTTGTTATTTTTTTGTTGTATATATATAATAATAAAGTAAATTAAAAA
TARGET=291,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01583 Ps_contig_mira-and-tgicl-ass_franssen_15065
SEQUENCE=TGTGGCCGGAAATAGAGACATTGAAGCAAATTAAAAGTTTTGTTTTGGGTGATTATATCATAAATTTTTGTTGTTGTTTTTTCTTTTGATATTCTATTCTACCTACGAAAATCATTCATTGCTGAACTGAGGGAAAATGTGGAAGTAGAAGATGAATGTTGTATTGTTTCCACACGGACACTCTTATTCTTTATACTTTGTGCAATTGTTAAACGTTGTTGATTTTTTTGTTGTATATATATAATATAAAGTAAAATTTAATAATTATACATAATGGTTGATACCTATGACCAAATTCCAAGAA
TARGET=234,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01584 Ps_contig_mira-and-tgicl-ass_franssen_15080
SEQUENCE=CTAGATTCAGCAGGCCATTGGACTGNGAAACAAATTAGCATCTCTTGCAAGCTTTAATGCAAATTTCTAGATCATGTCTTGCCACAAGCCTWGGACTGCAGTATTCAAATTTTCTTTTGGAGCAGATTCACCTGATTAACTAGCATAGCAATCTTCCGCTGTTTCGCAGGCCATTTTCCACTTAAAAATTGGAAAAGTGGATAGTAATCACCATAATAAATAACATGCGTCAAATCCAAAGCAATAACAGCATTGAAAGCTTCAATCAAAGCCTTAAGCTCCACAGCAATTTTTACTAGTTCCATTCCCAACCACGGATTTAGAAACTTCAAATATCAAATTATCAGTCAAATCACAAACCGCAACACCAATCCCAGCCAACGAAACTTTCTCCCCTCWCACAGTCTCTTCACTCACTAAACCCTTAAAATACACCCTAACAGACGCACCTTCAACGTTACCACTCTTCAACCCAGACGAACTCGAACTCCCCTCACCGAACGGTTTCTCGAAATTATCGCCCCATTCATCCCAATCTTCTCCAGGAAAATTGAGAATCTCCGACGCCATTTTCTGATCGTGAATCCGACGGTTGATATCAACCTTCGCCTCGCGCATTATTCTCTCGCTCTGTTCTCTGTCGTTCATCTCGATCTCCATCCTTGCGAGCTCTTCCAACTGGAGAGACGGTGCTTTGGAAACGTCGTCGTCGTCCAAGGGGAGTTCTACGAGTATAGCACCCATGGAGGATGAGGGATGATCGGTGAGGGAAGCGGCAAGGGCTTCCTGTAACTGGAGATGGAAAGCGAAATCGAGATCGGATTCCATGGATTCCGCCGCCGTGAGTTCCCGGCGTTGCGCTGTTAATAAGAAGTGCAGGTCTTCCGTTGAGTCCATGGAATGTGACTCCCCGAC
TARGET=702,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01585 Ps_contig_mira-and-tgicl-ass_franssen_15103
SEQUENCE=TGGACACAAGCACTATCAACTATCAACACATCCAATTCACCGTAAAGAAAATGTCACTCTTTTCCAATAGTATCTTTGGTCGAAGAAGATCAAAATCACCACAAGATCATCATCATCATCATCACCAATCAAGGAACAACCACCCATCACACCAAACCCATGGTTATGAAGTTTCTCAAACCCACACACCCCATATAACACTACCACCAAATTTCCACGAGCCATCACCAATTGTCGACACCATTGACATAGAGTGGAAAGAAACCCCTAGAAAGAACTCATGTTTTCAAAGCACATCTTCCGGGG
TARGET=106,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3676
PRIMER_LEFT_PENALTY=0.139129
PRIMER_RIGHT_PENALTY=0.228464
PRIMER_LEFT_SEQUENCE=CACATCCAATTCACCGTAAAGA
PRIMER_RIGHT_SEQUENCE=ATAACCATGGGTTTGGTGTGAT
PRIMER_LEFT=26,22
PRIMER_RIGHT=166,22
PRIMER_LEFT_TM=59.861
PRIMER_RIGHT_TM=60.228
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.3925
PRIMER_LEFT_1_PENALTY=0.139129
PRIMER_RIGHT_1_PENALTY=0.253332
PRIMER_LEFT_1_SEQUENCE=CACATCCAATTCACCGTAAAGA
PRIMER_RIGHT_1_SEQUENCE=GTTATATGGGGTGTGTGGGTTT
PRIMER_LEFT_1=26,22
PRIMER_RIGHT_1=198,22
PRIMER_LEFT_1_TM=59.861
PRIMER_RIGHT_1_TM=59.747
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.4139
PRIMER_LEFT_2_PENALTY=0.139129
PRIMER_RIGHT_2_PENALTY=0.274768
PRIMER_LEFT_2_SEQUENCE=CACATCCAATTCACCGTAAAGA
PRIMER_RIGHT_2_SEQUENCE=CTCGTGGAAATTTGGTGGTAGT
PRIMER_LEFT_2=26,22
PRIMER_RIGHT_2=220,22
PRIMER_LEFT_2_TM=59.861
PRIMER_RIGHT_2_TM=60.275
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.4411
PRIMER_LEFT_3_PENALTY=0.139129
PRIMER_RIGHT_3_PENALTY=0.301926
PRIMER_LEFT_3_SEQUENCE=CACATCCAATTCACCGTAAAGA
PRIMER_RIGHT_3_SEQUENCE=GGTGTGTGGGTTTGAGAAACTT
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=189,22
PRIMER_LEFT_3_TM=59.861
PRIMER_RIGHT_3_TM=60.302
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.5706
PRIMER_LEFT_4_PENALTY=0.139129
PRIMER_RIGHT_4_PENALTY=0.431486
PRIMER_LEFT_4_SEQUENCE=CACATCCAATTCACCGTAAAGA
PRIMER_RIGHT_4_SEQUENCE=CCACTCTATGTCAATGGTGTCG
PRIMER_LEFT_4=26,22
PRIMER_RIGHT_4=256,22
PRIMER_LEFT_4_TM=59.861
PRIMER_RIGHT_4_TM=60.431
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_01586 Ps_contig_mira-and-tgicl-ass_franssen_15108
SEQUENCE=ATTCAATTTTGATTTGTGGGTTGTTTTCATTTGAAGATCTTGAGGATTTGAGGATTGAATTGAGATAGGTTTTGTAGATTTTACTATGTAGTTTGGTGGAAATCATTGAAGAATGTGTGTGGAATTGGATTGGCTGTGGAATGGGTTTATTGGTTTGATTTGATTTGAATTTGTTGTGATTGAGAAGAAATGGAAGGGAATTTATCACAAGGAGGTATAATTCAAGGTGGTGGTAGTGGTTCTTTTGGTTTGCCTGGATCAATGCGAGTTCATCGTCAAGCACAGATGAACCAACATCAAGCTCATCCTTGTCAAGGGTCTGCGGGGCATTCGTCGACTCGTGAAGGTTTTCCTCT
TARGET=154,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1001
PRIMER_LEFT_PENALTY=0.095771
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=GAATGTGTGTGGAATTGGATTG
PRIMER_RIGHT_SEQUENCE=AAGGATGAGCTTGATGTTGGTT
PRIMER_LEFT=110,22
PRIMER_RIGHT=309,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=200
PRIMER_PAIR_PENALTY_1=0.1680
PRIMER_LEFT_1_PENALTY=0.163658
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=GAAGAATGTGTGTGGAATTGGA
PRIMER_RIGHT_1_SEQUENCE=AAGGATGAGCTTGATGTTGGTT
PRIMER_LEFT_1=107,22
PRIMER_RIGHT_1=309,22
PRIMER_LEFT_1_TM=59.836
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.1987
PRIMER_LEFT_2_PENALTY=0.163658
PRIMER_RIGHT_2_PENALTY=0.035021
PRIMER_LEFT_2_SEQUENCE=GAAGAATGTGTGTGGAATTGGA
PRIMER_RIGHT_2_SEQUENCE=AGGCAAACCAAAAGAACCACTA
PRIMER_LEFT_2=107,22
PRIMER_RIGHT_2=254,22
PRIMER_LEFT_2_TM=59.836
PRIMER_RIGHT_2_TM=60.035
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=0.2204
PRIMER_LEFT_3_PENALTY=0.095771
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=GAATGTGTGTGGAATTGGATTG
PRIMER_RIGHT_3_SEQUENCE=CTTGACAAGGATGAGCTTGATG
PRIMER_LEFT_3=110,22
PRIMER_RIGHT_3=315,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.2507
PRIMER_LEFT_4_PENALTY=0.163658
PRIMER_RIGHT_4_PENALTY=0.087031
PRIMER_LEFT_4_SEQUENCE=GAAGAATGTGTGTGGAATTGGA
PRIMER_RIGHT_4_SEQUENCE=GGCAAACCAAAAGAACCACTAC
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=253,22
PRIMER_LEFT_4_TM=59.836
PRIMER_RIGHT_4_TM=59.913
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=5.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_01587 Ps_contig_mira-and-tgicl-ass_franssen_15132
SEQUENCE=GTCGGGGATCCATTTCTCATTCTTTTTCTTTCTGTAAACAAAAAGAGAGTGCGAGGGTGTGAACAGTGTTTGATTCCACCCTCAAGAATCATACTATTCAAATCGAAAACTAAAGTTTTGTAATGGCTTCAAGACCCATTGTTCCTCAACAACAACAACCTAGAGGCGATGCTGCTGTGGGGGGAGTGAAACAGCAGAAGAAGAACCTTGCAGCAGGCGAGGGAAGGAACCGTAGAGCAGCACTTCATGATATTGGTAATGTAGTCACGGCAGTAAGAGGTGTTGAAGTCAAGCCAAATCGTCCCATAACAAGGAATTTCTGTGCACAACTACTTGCCAATGCCCAAGCAGCTGCTGCTGAAAATAACAAGAAACAGGTCTGTCCTCCTCCTGCTGCTGTCAAGGGAGTTCCAGTGGCTAGAAGAGTGGCAGTGGCCCCTAAACCTGTTCAGAAGAAAGTCACTGCAAAACCAAAGCCTGTTGAAGTGATTGATGTAATAAGTTCTGAAGAAGAGAGCAGTAAAGAAAAGTCTGTGCTTAACAAAAAGGGAGGAGAGGTTAATTCCAGGAAGAAATCATCACGCACTCTTACTTCTGTTCTAACAGCTAGAAGCAAGGCTGCATGTGGTTTGACTAACAAACCTAAGGAGATTGTTGACATTGATGCTGCTGATGTTAACAATGAACTTGCTGCTGTTGAGTATCTTGATGACATTTACAAGTTCTACAAGTTAGTTGAGAATGAGAGCCGTCCACATGATTACATGGACTCACAACCTGAGATTAACGACAAGATGAGAGCTATACTCATTGACTGGCTAGTAGATGTTCACACAAAATTCGAACTTTCGCCAGAAGCCCTTTACCTCACAATCAACATAATTGATAGATTCTTAGCTATCAGCCTTGTGTCAAGAAGGGAACTTCAATTGGTTGGCATCAGTGCTATGTTGATGGCATCAAAGTACGAAGAAATCTGGCCACCTGAGGTTAATGACTTTGTGTGCCTCTCAGATAGAGCCTACACTCATGAACAAATACTCATCATGGAGAAAACCATCCTAGGGAAGCTTGAATGGACACTGACAGTGCCTACACCATTTGTTTTCTTAGTCCGATTCCTCAAGGCGGCTTCGGTGTCCTTACCTTCTTCTGATCTCGCTTTAGAGAACATGGCTCATTTTCTTTCTGAGCTGGGAATGATGCATTATGCTACCTTGATGTACAGCCCCTCAATGATGGCTGCAGCAGCAGTTTATGCAGCAAGGTGCACATTGAACAAAAGTCCTGTTTGGGATGAAACATTGACGATGCACACTGGTTACTCAGAAGAAGAATTGATGGGTTGTGCTAGATTGTTGGTGAGTTTTCACAGTGCTAGTGGGAGTGGAAAACTCAAGGGTGTCTATAAGAAGTATGCTGATCCTCAGAAAGGCGCTGTTGCTGTTCTTCCACCGGCGAAAAACCTTGTGTCATCTGCCGCAGTTGGTTCTTAAATTTGTCATTGAAATAAACAAAGGAAGCTGCCTGAGTTTTTAGGTTTATGTAGGGCTCTCAGCAATAACCATTTGTTATTGTAATTTTTTTCTTTCTTTTCATTGTTTAGACCCTTTTCAGATGTTATGAAATCAAACACTTTCATATATAAAGCTTCCTCATTTTCCTTATTTTCATTCTGACATCATAGAATTGTATAATCTCTTAAAA
TARGET=147,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3078
PRIMER_LEFT_PENALTY=0.267873
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=AACAGTGTTTGATTCCACCCTC
PRIMER_RIGHT_SEQUENCE=ATTTGGCTTGACTTCAACACCT
PRIMER_LEFT=61,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=60.268
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.3287
PRIMER_LEFT_1_PENALTY=0.267873
PRIMER_RIGHT_1_PENALTY=0.060824
PRIMER_LEFT_1_SEQUENCE=AACAGTGTTTGATTCCACCCTC
PRIMER_RIGHT_1_SEQUENCE=GCAAGGTTCTTCTTCTGCTGTT
PRIMER_LEFT_1=61,22
PRIMER_RIGHT_1=210,22
PRIMER_LEFT_1_TM=60.268
PRIMER_RIGHT_1_TM=60.061
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=150
PRIMER_PAIR_PENALTY_2=0.3287
PRIMER_LEFT_2_PENALTY=0.267873
PRIMER_RIGHT_2_PENALTY=0.060824
PRIMER_LEFT_2_SEQUENCE=GAACAGTGTTTGATTCCACCCT
PRIMER_RIGHT_2_SEQUENCE=GCAAGGTTCTTCTTCTGCTGTT
PRIMER_LEFT_2=60,22
PRIMER_RIGHT_2=210,22
PRIMER_LEFT_2_TM=60.268
PRIMER_RIGHT_2_TM=60.061
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=151
PRIMER_PAIR_PENALTY_3=0.3390
PRIMER_LEFT_3_PENALTY=0.299089
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=TTTGATTCCACCCTCAAGAATC
PRIMER_RIGHT_3_SEQUENCE=ATTTGGCTTGACTTCAACACCT
PRIMER_LEFT_3=68,22
PRIMER_RIGHT_3=298,22
PRIMER_LEFT_3_TM=60.299
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=231
PRIMER_PAIR_PENALTY_4=0.3599
PRIMER_LEFT_4_PENALTY=0.299089
PRIMER_RIGHT_4_PENALTY=0.060824
PRIMER_LEFT_4_SEQUENCE=TTTGATTCCACCCTCAAGAATC
PRIMER_RIGHT_4_SEQUENCE=GCAAGGTTCTTCTTCTGCTGTT
PRIMER_LEFT_4=68,22
PRIMER_RIGHT_4=210,22
PRIMER_LEFT_4_TM=60.299
PRIMER_RIGHT_4_TM=60.061
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_01588 Ps_contig_mira-and-tgicl-ass_franssen_15139
SEQUENCE=AAGGTTGAAGCAGTAGAAAACAGATCATTCAGCCATATATTGCTCCAATGTTTTTATCAGAATTTCTTCCAGAAAATATTCTGAAATCATAAGTTTCTAGCACTGTTAAATACGAAATATACACCTGTACAGGCAACACATGACCAAGTTTGATATGTATGTATGTCAATGATAGAAACAAAATTTAAAATACAATTAAACCACTTCAAAATTCAAGTCACATCCAGAAGCAAGAATGTTGCAGATATGGCTGGTGGGAGTGGCATGTAGATGGATAAATCCACTTTTAGTAGTATGTAAACTTTTGCAAGTTTGTTGTCTTAAGCCGCATGAATGCATTACCTGCAATCATACTGACAAAAGAAATAGAAACATTGANAAATTAAACAATCCCTGTTCTGATCTATACAATAA
TARGET=154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6634
PRIMER_LEFT_PENALTY=0.474295
PRIMER_RIGHT_PENALTY=0.189072
PRIMER_LEFT_SEQUENCE=GTACAGGCAACACATGACCAAG
PRIMER_RIGHT_SEQUENCE=ATGCGGCTTAAGACAACAAACT
PRIMER_LEFT=126,22
PRIMER_RIGHT=330,22
PRIMER_LEFT_TM=60.474
PRIMER_RIGHT_TM=60.189
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.7340
PRIMER_LEFT_1_PENALTY=0.474295
PRIMER_RIGHT_1_PENALTY=0.259704
PRIMER_LEFT_1_SEQUENCE=GTACAGGCAACACATGACCAAG
PRIMER_RIGHT_1_SEQUENCE=TGCTTCTGGATGTGACTTGAAT
PRIMER_LEFT_1=126,22
PRIMER_RIGHT_1=231,22
PRIMER_LEFT_1_TM=60.474
PRIMER_RIGHT_1_TM=59.740
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=0.7340
PRIMER_LEFT_2_PENALTY=0.474295
PRIMER_RIGHT_2_PENALTY=0.259704
PRIMER_LEFT_2_SEQUENCE=GTACAGGCAACACATGACCAAG
PRIMER_RIGHT_2_SEQUENCE=ACATTCTTGCTTCTGGATGTGA
PRIMER_LEFT_2=126,22
PRIMER_RIGHT_2=238,22
PRIMER_LEFT_2_TM=60.474
PRIMER_RIGHT_2_TM=59.740
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=0.7340
PRIMER_LEFT_3_PENALTY=0.474295
PRIMER_RIGHT_3_PENALTY=0.259704
PRIMER_LEFT_3_SEQUENCE=GTACAGGCAACACATGACCAAG
PRIMER_RIGHT_3_SEQUENCE=CATTCTTGCTTCTGGATGTGAC
PRIMER_LEFT_3=126,22
PRIMER_RIGHT_3=237,22
PRIMER_LEFT_3_TM=60.474
PRIMER_RIGHT_3_TM=59.740
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=112
PRIMER_PAIR_PENALTY_4=0.9058
PRIMER_LEFT_4_PENALTY=0.474295
PRIMER_RIGHT_4_PENALTY=0.431486
PRIMER_LEFT_4_SEQUENCE=GTACAGGCAACACATGACCAAG
PRIMER_RIGHT_4_SEQUENCE=CTTGCTTCTGGATGTGACTTGA
PRIMER_LEFT_4=126,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=60.474
PRIMER_RIGHT_4_TM=60.431
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_01589 Ps_contig_mira-and-tgicl-ass_franssen_15147
SEQUENCE=TTCTCGTTTCTCTATKCTCCTCTTCCTACTCTTTATTTATACCCCATTTACACCTGCACCCTAGTTCCATTCCCTTCTGCTTCACCTCTTCTCCTTCTTCTCCTCATGTGAACGCTATTTCACTACAATAATACTACAACACTTCAAAATAAACATGAGTTGCAACGGTTGCCGTGTCCTTAGAAAGGGTTGTAGTGAGTCTTGTATCTTACGCCCTTGTATTCAATGGATCGATACCCCTGAATCACAAGGCTATGCCACTCTCTTCGTAGCCAAGTTTTTTGGTCGTGCTGACCTTATGTCTTTCATTTCTAACGTCCCTCAACCACAAAGACCCGCTTTGTTTCAATCTCTCTTGTTTGAAGCGTGTGGACGAACGGTTAATCCCGTTAACGGTGCTGTTGGCCTTTTATGGACAGGAAACTGGCACGTGTGTCAAGCGGCTGTTGAAACCGTTCTCCGCGGCGGAACCCTTGGGCCTATACCGGAGCTTCTATGTTTAGATGACGCATCCGAAGGGGAGGTCACGTGTAACGACGCGCGGAGGATCCGAGATCCGAACTCGAGTTTCCGGTTCGTGAGTTCCGGTGGTAAACGCAAGCGATTGAGTGAAGCTGCGAAGATTCAGACTCAGACGACGACTGATCTTAATCTCCGGTTGACTCCATGTTTTCTTCAGAACGCGTTGACCAACAGTTACCGGAGGGACGTTTGTCGGCAGGGGACACCGTCGTTGAATTCTGAGGAATCTGTGACGACGACGACGACGTGTTTGGAGAGTGGGAACGGAGATGAGTACGTTCACGACGGAGACCGGAAAGTTCTTAACCTTTTTGTTTGAGGGATATTTCTTCTTTATGAGATTCGTGTTTTTTGTTTCTGGATCGTGCGATTAAGGGCTTGTGAGCACGCTATTTTTGTCTTTTTTTTAGTGATTTCTTTTACCGGAAAATAATTCCGGTGAGATTTTGTAAAACGCCGGCACATGATTTTTCTCTGTTCTTTTATTAACAGGGAGAAAGATGAAGGTTAGTCGGTGTAAGGATTTTTTTTTTTAAAAAATTTAAAA
TARGET=754,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01590 Ps_contig_mira-and-tgicl-ass_franssen_15160
SEQUENCE=GTYYRGGGTAATTCCTTAATTTTCTAAACCCTATTTTTTCTTTCTTTCACCCAAAGTTTCAATCTTTAACCCCAATTCAACCTTCTTCTTCATCCATGGCTACTTGTGTTGATTCTTCAACCCCTCCCCTTTCTCGCAAACCAAACGTTTCCCAACCCGATGATTGTTCCTTCAACTTCGCCAAATTTTTCCAACCCACTTTCTCAGATCGTGTTTCATCCATACCCATCTCAACCAATCATGAAAAAGCCTCGGTTTTGGAGGATTTTGAACAGGTTCATGATAAGGTTCAAAATGAGGTTGATGATGATGCTAATGTTAGGTTTGATTTATGGTTGAAGATTCAAGAAGAAGCAAGGTTAGATTTAGATCAAGAACCAATTTTATCTAGCTACTATTTCAGTTCAATTTTATCTCACAAGTCATTCGAAAGTGCTTTAGCAAATCAACTCTCGATTCATTTGAGTAATTTGAGTCTTTCTAGCACAACCCTTTTCGATATTTTCAAGAGTGTTATTGATGATGATGAAGATATCATAAACGCTATGAAATTCGACATCGAAGCAGTGAAAGAAAGAGACCCTGCATGTATAAGCTATGTTCATTGTTTGTTAAACTTCAAAGGCTTTCTAGCAATGCAAGCTCATAGGGTAGCTCACAAATTATGGTTACAAGGTAGAAAAGTTTTAGCTTTGTTGATTCAAAATCGGGTATCGGAGGTTTTCGCAGTTGACATTCATCCCGGCGCTAAGATCGGAAGAGGGATTCTGCTTGATCATGCAACGGGACTAGTTGTTGGCGAAACGGCTGTGATCGGGAACAATGTTGCGATTTTGCATAATGTTACGTTAGGCGGAACGGGTAAAGCTTGTGGCGATCGACATCCGAAAATCGCAGATGGTGTTTTGATTGGTGCAGGGACATGTATTTTAGGGAATGTTAGTATTGGTGATGGTGCTAAGATTGGTGCTGGTTCTGTTGTTTTGAAAGATGTTCCTCCAAGAACTACTGCTGTTGGAAATCCTGCTAAGTTGATTGGTGGAAAAGATAACCCTATTATGTTGGATAAGATTCCTAGCTTTACTATGGATCATACTTCTTATATTTCTGATTGGTCTGATTATGTCATTTAAGAACATGGACACACAGGAACTGGTTTGGATGTTGATTCAGACGAATCAACGTCCAAAAACGCGTTACTGTGTGTATAGTTAAATAAGTTGTTTAGAGATTAATCTTGTTATATGTCAGTAGATTTTTTTTATTAGATAATTTTGGTTGGGTATTTTGTTTGTTTGTTGTATAAGAGAGTATTTAGATTCTCCTGTTAACTACAAAAACATGCCTCTTAAAACCATGTATGTTTGCAAGAACCACTCTTTATTTGTATTTTATGTGCTTTAGCATATATATGATATATGAAAATGAATTTTGCTTGTTTTBCATTCATTTCTTTTTTATTAGCAT
TARGET=37,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01591 Ps_contig_mira-and-tgicl-ass_franssen_15160
SEQUENCE=GTYYRGGGTAATTCCTTAATTTTCTAAACCCTATTTTTTCTTTCTTTCACCCAAAGTTTCAATCTTTAACCCCAATTCAACCTTCTTCTTCATCCATGGCTACTTGTGTTGATTCTTCAACCCCTCCCCTTTCTCGCAAACCAAACGTTTCCCAACCCGATGATTGTTCCTTCAACTTCGCCAAATTTTTCCAACCCACTTTCTCAGATCGTGTTTCATCCATACCCATCTCAACCAATCATGAAAAAGCCTCGGTTTTGGAGGATTTTGAACAGGTTCATGATAAGGTTCAAAATGAGGTTGATGATGATGCTAATGTTAGGTTTGATTTATGGTTGAAGATTCAAGAAGAAGCAAGGTTAGATTTAGATCAAGAACCAATTTTATCTAGCTACTATTTCAGTTCAATTTTATCTCACAAGTCATTCGAAAGTGCTTTAGCAAATCAACTCTCGATTCATTTGAGTAATTTGAGTCTTTCTAGCACAACCCTTTTCGATATTTTCAAGAGTGTTATTGATGATGATGAAGATATCATAAACGCTATGAAATTCGACATCGAAGCAGTGAAAGAAAGAGACCCTGCATGTATAAGCTATGTTCATTGTTTGTTAAACTTCAAAGGCTTTCTAGCAATGCAAGCTCATAGGGTAGCTCACAAATTATGGTTACAAGGTAGAAAAGTTTTAGCTTTGTTGATTCAAAATCGGGTATCGGAGGTTTTCGCAGTTGACATTCATCCCGGCGCTAAGATCGGAAGAGGGATTCTGCTTGATCATGCAACGGGACTAGTTGTTGGCGAAACGGCTGTGATCGGGAACAATGTTGCGATTTTGCATAATGTTACGTTAGGCGGAACGGGTAAAGCTTGTGGCGATCGACATCCGAAAATCGCAGATGGTGTTTTGATTGGTGCAGGGACATGTATTTTAGGGAATGTTAGTATTGGTGATGGTGCTAAGATTGGTGCTGGTTCTGTTGTTTTGAAAGATGTTCCTCCAAGAACTACTGCTGTTGGAAATCCTGCTAAGTTGATTGGTGGAAAAGATAACCCTATTATGTTGGATAAGATTCCTAGCTTTACTATGGATCATACTTCTTATATTTCTGATTGGTCTGATTATGTCATTTAAGAACATGGACACACAGGAACTGGTTTGGATGTTGATTCAGACGAATCAACGTCCAAAAACGCGTTACTGTGTGTATAGTTAAATAAGTTGTTTAGAGATTAATCTTGTTATATGTCAGTAGATTTTTTTTATTAGATAATTTTGGTTGGGTATTTTGTTTGTTTGTTGTATAAGAGAGTATTTAGATTCTCCTGTTAACTACAAAAACATGCCTCTTAAAACCATGTATGTTTGCAAGAACCACTCTTTATTTGTATTTTATGTGCTTTAGCATATATATGATATATGAAAATGAATTTTGCTTGTTTTBCATTCATTTCTTTTTTATTAGCAT
TARGET=518,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01592 Ps_contig_mira-and-tgicl-ass_franssen_15160
SEQUENCE=GTYYRGGGTAATTCCTTAATTTTCTAAACCCTATTTTTTCTTTCTTTCACCCAAAGTTTCAATCTTTAACCCCAATTCAACCTTCTTCTTCATCCATGGCTACTTGTGTTGATTCTTCAACCCCTCCCCTTTCTCGCAAACCAAACGTTTCCCAACCCGATGATTGTTCCTTCAACTTCGCCAAATTTTTCCAACCCACTTTCTCAGATCGTGTTTCATCCATACCCATCTCAACCAATCATGAAAAAGCCTCGGTTTTGGAGGATTTTGAACAGGTTCATGATAAGGTTCAAAATGAGGTTGATGATGATGCTAATGTTAGGTTTGATTTATGGTTGAAGATTCAAGAAGAAGCAAGGTTAGATTTAGATCAAGAACCAATTTTATCTAGCTACTATTTCAGTTCAATTTTATCTCACAAGTCATTCGAAAGTGCTTTAGCAAATCAACTCTCGATTCATTTGAGTAATTTGAGTCTTTCTAGCACAACCCTTTTCGATATTTTCAAGAGTGTTATTGATGATGATGAAGATATCATAAACGCTATGAAATTCGACATCGAAGCAGTGAAAGAAAGAGACCCTGCATGTATAAGCTATGTTCATTGTTTGTTAAACTTCAAAGGCTTTCTAGCAATGCAAGCTCATAGGGTAGCTCACAAATTATGGTTACAAGGTAGAAAAGTTTTAGCTTTGTTGATTCAAAATCGGGTATCGGAGGTTTTCGCAGTTGACATTCATCCCGGCGCTAAGATCGGAAGAGGGATTCTGCTTGATCATGCAACGGGACTAGTTGTTGGCGAAACGGCTGTGATCGGGAACAATGTTGCGATTTTGCATAATGTTACGTTAGGCGGAACGGGTAAAGCTTGTGGCGATCGACATCCGAAAATCGCAGATGGTGTTTTGATTGGTGCAGGGACATGTATTTTAGGGAATGTTAGTATTGGTGATGGTGCTAAGATTGGTGCTGGTTCTGTTGTTTTGAAAGATGTTCCTCCAAGAACTACTGCTGTTGGAAATCCTGCTAAGTTGATTGGTGGAAAAGATAACCCTATTATGTTGGATAAGATTCCTAGCTTTACTATGGATCATACTTCTTATATTTCTGATTGGTCTGATTATGTCATTTAAGAACATGGACACACAGGAACTGGTTTGGATGTTGATTCAGACGAATCAACGTCCAAAAACGCGTTACTGTGTGTATAGTTAAATAAGTTGTTTAGAGATTAATCTTGTTATATGTCAGTAGATTTTTTTTATTAGATAATTTTGGTTGGGTATTTTGTTTGTTTGTTGTATAAGAGAGTATTTAGATTCTCCTGTTAACTACAAAAACATGCCTCTTAAAACCATGTATGTTTGCAAGAACCACTCTTTATTTGTATTTTATGTGCTTTAGCATATATATGATATATGAAAATGAATTTTGCTTGTTTTBCATTCATTTCTTTTTTATTAGCAT
TARGET=1287,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01593 Ps_contig_mira-and-tgicl-ass_franssen_15179
SEQUENCE=TATTAAGAATTTGCCATTTGAGCCTACTGTTCTACTAGTATTGGAGACCCTCAAGAGTTTTGCTCGACTTCATGGAGATATTGATCTTGAAGACCATGTTGAAGAGTTGATAGTTAGTCTTGACCCGTCAAAGGCGGTTGCCAATAAGATCCCTACCCCGCCTCCCAAAAAATATACTGCAATCAGCATGCTTGATGGCAGGAACAGAATCGTTGAGTATAAGAATCCTACTCTTTACAAGGATGACGAGAAGTTGAAGGCTTTGAGCAGTATGAAAGACGCCGGATATGTTCCTGATACAAGATATGTTCTTCACGACATTGATCAGGAAGCAAAGGAGCAGGCCTTGCTCTACCACAGTGAACGTCTAGCAATCGCATATGGTCTTATTAGTACTCCACCTAGAACACCTCTTAGGATTATCAAGAACCTTCGTGTCTGCGGTGATTGCCACAATGCCATCAAGATCATGTCTAGGATTGTAGGGAGGGAACTGATTGTCAGAGATAACAAAAGATTTCATCATTTCAAAGATGGAAAATGCTCTTGTGGGGATTACTGGTGAAGGAATCATTTCTCCAAATCCTATAATATCACGGAGTATATAAGTTTCTAGGCGCTGTCCGGTTCTGAATTGTGTGCCTGGAAGACAGATGGTGTGCGCGGAAGCTTTCAATACTGCTAGGCATAGGAGATGAATGGATTATGTACTATGCTCTGACTTATAATGTATTTGCTTCTTGTTCAGATACCAGCACTATTCCCTGTCTTCTATTCCCTGCTATATACTCATAAGGTTTTATTTATTTAATTATTTTTTGGTTCTAGTTCTTACCACTGCAAATTGCTCCACTTTGATCCATGCAAAGTATGTAGGACAAGTTTGCTTTACGAAAAGTGTTTTGGAACATTGTCTTGGAACGGTTGTTTGTTATTTTTAGTAAGGAAAAAAAAATAGTATTTTTTAAA
TARGET=799,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6755
PRIMER_LEFT_PENALTY=0.081801
PRIMER_RIGHT_PENALTY=0.593677
PRIMER_LEFT_SEQUENCE=GCTAGGCATAGGAGATGAATGG
PRIMER_RIGHT_SEQUENCE=GAGCAATTTGCAGTGGTAAGAA
PRIMER_LEFT=680,22
PRIMER_RIGHT=849,22
PRIMER_LEFT_TM=60.082
PRIMER_RIGHT_TM=59.406
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.7037
PRIMER_LEFT_1_PENALTY=0.081801
PRIMER_RIGHT_1_PENALTY=0.621889
PRIMER_LEFT_1_SEQUENCE=GCTAGGCATAGGAGATGAATGG
PRIMER_RIGHT_1_SEQUENCE=CCAAGACAATGTTCCAAAACAC
PRIMER_LEFT_1=680,22
PRIMER_RIGHT_1=918,22
PRIMER_LEFT_1_TM=60.082
PRIMER_RIGHT_1_TM=59.378
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.7623
PRIMER_LEFT_2_PENALTY=0.081801
PRIMER_RIGHT_2_PENALTY=0.680486
PRIMER_LEFT_2_SEQUENCE=GCTAGGCATAGGAGATGAATGG
PRIMER_RIGHT_2_SEQUENCE=ACAACCGTTCCAAGACAATGTT
PRIMER_LEFT_2=680,22
PRIMER_RIGHT_2=927,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=60.680
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=0.7806
PRIMER_LEFT_3_PENALTY=0.186949
PRIMER_RIGHT_3_PENALTY=0.593677
PRIMER_LEFT_3_SEQUENCE=GGGATTACTGGTGAAGGAATCA
PRIMER_RIGHT_3_SEQUENCE=GAGCAATTTGCAGTGGTAAGAA
PRIMER_LEFT_3=551,22
PRIMER_RIGHT_3=849,22
PRIMER_LEFT_3_TM=60.187
PRIMER_RIGHT_3_TM=59.406
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.8489
PRIMER_LEFT_4_PENALTY=0.226971
PRIMER_RIGHT_4_PENALTY=0.621889
PRIMER_LEFT_4_SEQUENCE=CCAGCACTATTCCCTGTCTTCT
PRIMER_RIGHT_4_SEQUENCE=CCAAGACAATGTTCCAAAACAC
PRIMER_LEFT_4=751,22
PRIMER_RIGHT_4=918,22
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=59.378
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=168
=
PRIMER_SEQUENCE_ID=SSR_01594 Ps_contig_mira-and-tgicl-ass_franssen_15185
SEQUENCE=GGTTTTAAAATTAAGTAAGAATCATTTCAAGTTCAAAGTTCTTGAAAACTAGGAGCAACAAACTAAATATACTTGATTCACACACACACAGATATGCATACTCTTCAAACATGAAAACTTTAATTGCTTGAATGTCTTCAAGTTCATGCTGGTGCTTTGACAGTAATAGATGGGAAATCCATCACCACAATCTTCTCAAGAGCAGGTGAAAATACCTTGGAGAACTCAACATGATTAGGGTGAAGCTCAAATGCATTCAAAGCCTCTTTCTCATTGAATGTCATCAAAAAGGCATGAGTAAAACCTTGTCTCAGCATGTCATGGCTTTCAATGTCTTTCCCCCATTCAAAGGACTTGACATGGTCAATCCCAGAAACCATCTTCTCCAAATCTTGGATGAGTTCTTCAACTTTGACACCATCCTTGAACTTAACAATTACATAATGATTGTAAGTCCCCATGACTGCAATACAATTTTTTATCCCCGCGWTACTCTGCGTTGATAACCAACTAGTT
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01595 Ps_contig_mira-and-tgicl-ass_franssen_15186
SEQUENCE=AATTAAAGTTTTCATGTTTGAAGAGTATGCATATCTGTGTGTGTGAATCAAGTATATTTAGTTTGTTGCTCCTAGTTTTCAAGCAATTTGAACTTGAAATGATCTTCTATTTTACAATTAATGGGATGTTCTAGTTATTGGTATTTATTAATATACTAAGTATTTGTGTGAMYTGATTTATTTGA
TARGET=36,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01596 Ps_contig_mira-and-tgicl-ass_franssen_15190
SEQUENCE=ATCCATTTGAAATTTTCCATATCTACTTTATTTATTTATTTTTGTCYCAACAGAAGAAAGGTTTTCCTGGCTGGATGGCCGAACTGAATTTCCTGTTGCAGTTGCAGACATT
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01597 Ps_contig_mira-and-tgicl-ass_franssen_15193
SEQUENCE=ATGAGTTAACTCGTTATTTATTTATTTTTAATATGTTTTTCTTGTGGAGTTCAAAAGGTGAGATGAGTAGCAAGTGCCTTTTTTTTCTTTCTCTTTATTTTATTGGTTTATTAGACACCATCCACTAGTACTGATTATCATAATTATTTTATCAATCGTCTAAT
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01598 Ps_contig_mira-and-tgicl-ass_franssen_15199
SEQUENCE=GTCTATAGATGCTTCGACAGACGAATGGCTACAGGGAACTGCATATACTCAGTCTCTTTCCAGCAAATCAAAGAAGAAGAAGTAGATGCATAGTTTTCCTTTTCCTCCAGTTCTTCAGCCAAACATGACTAGAGATGTAACACCATGTTAATTAGTTTGAGAGCGCGAGGAATTGTGGCTGAATGRTGACGACCTGTCCTTTTCTGTTGTAGTACTTTTAAAAGTAAACTTTTTTACCCTTTTTTCCCTTCCTATTGTAGTTTGAGTTACTTTTTAATAAAAACG
TARGET=71,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01599 Ps_contig_mira-and-tgicl-ass_franssen_15200
SEQUENCE=GGTCAAGTACAAAACCTATCTAAGAAATCAAAGAGTTTTTAGTTTTTCTTTTTTCCCTTTTAAAGWKTTGAAGTTGGAACTAGGTCTATAGATGCTTCGACAGACGAATGGCTACAGGGAACTGCATATACTCAGTCTCTTTCCAGCAAATCAAAGAAGAAGAAGTAGATGCATAGTTTTCCTTTTCCTCCAGTTCTTCAGCCAAACATGACTAGAGATGTAACACCATGTTAATTAGTTTGAGAGCGCGAGGAATTGNGGAACTGAATGATGACACCTGCCTTTTCTGTTGTAGACTTTAAAAGTAAACTTTTTACCCTTTTCCCTCTAG
TARGET=154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01600 Ps_contig_mira-and-tgicl-ass_franssen_15206
SEQUENCE=CGTTAATCTCTTCTTCCCACGTGGAACCAACCAACCTGGACCTCCGGTCAAAACGAAGATATTCCAGCAGAAAGTGTAAGAATATCAGCACAAGAAACTYTGTTTGGACAAGCACTTTCTACAGCTGTTTTAATCTGATTTATAATATCTAAGCCTCTTAAGGAATTGATATTTGGGAAGGCTTCTTGTTCAGTCACAATGGTATCAGTTTTGTTCAACAAAACGGATGCATCACAACCTTGAACAAAGCAATCGTGAAAGTGGAGCCTGATGAGACTAGCAGGCATGCGAGGATCTGTCTTAGCAACTTTGAATAAGATTTGGTATACAATATAATGTAATTGGGACAAGTTTGACTGTAAAAAAATGGATTAAGTTGTGCATTTGAGGGGAAGGTTAATCCTCCAAACACAATTGCAACACAGCAGAGAGCTGTGGCTATGAGACGAAGGGGGTTCATATTTTCCCTTACTGATATTAGAGATGTGTGTTTGAAGTTTGTGCTTCCCGA
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01601 Ps_contig_mira-and-tgicl-ass_franssen_15207
SEQUENCE=GTCGTGGTGTAGATCACAATTTTTTCTTTATCATAAAAATAGTTATCGACATACCTGAGAGTGAAACTAGATCAAGTGTGGTGAGGCCTTGATCAGCAAAAGCAGATTTCAGTTCAGTTAAGCTGAAAGATGGACCTGGAAGGTTTTGATTAGCCAGAGTTTGATTTGCGGTTAAACTATCTCTTCTTCCCAGTGGAACCAACCAACCTGGACCTCCGGTCAAAACGGAAGATATTCCAGCAGAAAGTGTAAGAATATCAGCACAAAGAAACTTTTGTTTTGGACAAGCACTTTCTACAGCTTGTTTTAANCTGATTTATAATATCTAACG
TARGET=197,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2177
PRIMER_LEFT_PENALTY=0.122257
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=CTGGAAGGTTTTGATTAGCCAG
PRIMER_RIGHT_SEQUENCE=TCTGCTGGAATATCTTCCGTTT
PRIMER_LEFT=135,22
PRIMER_RIGHT=243,22
PRIMER_LEFT_TM=60.122
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=0.2177
PRIMER_LEFT_1_PENALTY=0.122257
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=CTGGAAGGTTTTGATTAGCCAG
PRIMER_RIGHT_1_SEQUENCE=TTCTGCTGGAATATCTTCCGTT
PRIMER_LEFT_1=135,22
PRIMER_RIGHT_1=244,22
PRIMER_LEFT_1_TM=60.122
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=110
PRIMER_PAIR_PENALTY_2=0.3061
PRIMER_LEFT_2_PENALTY=0.122257
PRIMER_RIGHT_2_PENALTY=0.183834
PRIMER_LEFT_2_SEQUENCE=CTGGAAGGTTTTGATTAGCCAG
PRIMER_RIGHT_2_SEQUENCE=TGGAATATCTTCCGTTTTGACC
PRIMER_LEFT_2=135,22
PRIMER_RIGHT_2=238,22
PRIMER_LEFT_2_TM=60.122
PRIMER_RIGHT_2_TM=60.184
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=0.3990
PRIMER_LEFT_3_PENALTY=0.215198
PRIMER_RIGHT_3_PENALTY=0.183834
PRIMER_LEFT_3_SEQUENCE=AAAGATGGACCTGGAAGGTTTT
PRIMER_RIGHT_3_SEQUENCE=TGGAATATCTTCCGTTTTGACC
PRIMER_LEFT_3=125,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=60.215
PRIMER_RIGHT_3_TM=60.184
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=0.4921
PRIMER_LEFT_4_PENALTY=0.122257
PRIMER_RIGHT_4_PENALTY=0.369874
PRIMER_LEFT_4_SEQUENCE=CTGGAAGGTTTTGATTAGCCAG
PRIMER_RIGHT_4_SEQUENCE=CTGCTGGAATATCTTCCGTTTT
PRIMER_LEFT_4=135,22
PRIMER_RIGHT_4=242,22
PRIMER_LEFT_4_TM=60.122
PRIMER_RIGHT_4_TM=59.630
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_01602 Ps_contig_mira-and-tgicl-ass_franssen_15213
SEQUENCE=CCGGGGATAGCATAGCATCATCCCTTTTTATTCTATCTTTTCTTGTCCCTTTCCCATTCACTTGATCTTCATTTTTCATCTCAAACACCACCATAGCCACTTCTCTTCTTTGAATTGCGTGCAAATTTAGCTACTTTTGTTCCTCTTGTTGCTTCTCTTACAAGATCTTATCTCACCCCAGCTTTGTTCCTAAGCTGAAATCTTTTATTTTATTTTACAAGATTCTCTATTTTTTTCTCATATAATGGTGACCCTTAATATTATTTACATATAATGGATTCCAATACCCAACAGAACCAACCCAATTCTGGGTTGCTTCGTTTTCGTTCTGCACCAAGTTCACTCTTTTCAAATCTCACACCTTCTTTTGTTGATGATAACAAAGGCACTAAAGATTTTTGGGATGATTCTGAATCAGAGAGATTGGTTTCAAGGTTTGTGAGTAACGATAATATTAATATGTCTGGTTCTTCTTGTTCTAAGGAGATGAGTGGAATGAATTCTGGTTATGGCGGCGGCGGCGGCTGTGGTAGTGGGTTGCCACCTCACTATCCTAGACACGGTTCTGCTGCAGCTTCAAGTTCTGCTATGGATGGTTCTTTTGGATTGGTCGGTTCATTGGGGATGAATAATGAAACATCTCATAAGACCTTTGGTTCAAATCTTCTTAGGCAGGGTAGTTCCCCTGCTGGTCTTTTCTCCAACATTTCATTTCAAAATGGGTTTGCTGCCATGAAGGGTGTGGGAA
TARGET=203,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2521
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.156479
PRIMER_LEFT_SEQUENCE=CAAGATCTTATCTCACCCCAGC
PRIMER_RIGHT_SEQUENCE=CATCCCAAAAATCTTTAGTGCC
PRIMER_LEFT=160,22
PRIMER_RIGHT=405,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.844
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.2868
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.156479
PRIMER_LEFT_1_SEQUENCE=TCTTATCTCACCCCAGCTTTGT
PRIMER_RIGHT_1_SEQUENCE=CATCCCAAAAATCTTTAGTGCC
PRIMER_LEFT_1=165,22
PRIMER_RIGHT_1=405,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.844
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.4835
PRIMER_LEFT_2_PENALTY=0.059762
PRIMER_RIGHT_2_PENALTY=0.423703
PRIMER_LEFT_2_SEQUENCE=ACACCACCATAGCCACTTCTCT
PRIMER_RIGHT_2_SEQUENCE=GGTTCTGTTGGGTATTGGAATC
PRIMER_LEFT_2=84,22
PRIMER_RIGHT_2=297,22
PRIMER_LEFT_2_TM=60.060
PRIMER_RIGHT_2_TM=59.576
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.4954
PRIMER_LEFT_3_PENALTY=0.071684
PRIMER_RIGHT_3_PENALTY=0.423703
PRIMER_LEFT_3_SEQUENCE=AAACACCACCATAGCCACTTCT
PRIMER_RIGHT_3_SEQUENCE=GGTTCTGTTGGGTATTGGAATC
PRIMER_LEFT_3=82,22
PRIMER_RIGHT_3=297,22
PRIMER_LEFT_3_TM=59.928
PRIMER_RIGHT_3_TM=59.576
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=216
PRIMER_PAIR_PENALTY_4=0.5036
PRIMER_LEFT_4_PENALTY=0.347129
PRIMER_RIGHT_4_PENALTY=0.156479
PRIMER_LEFT_4_SEQUENCE=CTTATCTCACCCCAGCTTTGTT
PRIMER_RIGHT_4_SEQUENCE=CATCCCAAAAATCTTTAGTGCC
PRIMER_LEFT_4=166,22
PRIMER_RIGHT_4=405,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=59.844
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_01603 Ps_contig_mira-and-tgicl-ass_franssen_15213
SEQUENCE=CCGGGGATAGCATAGCATCATCCCTTTTTATTCTATCTTTTCTTGTCCCTTTCCCATTCACTTGATCTTCATTTTTCATCTCAAACACCACCATAGCCACTTCTCTTCTTTGAATTGCGTGCAAATTTAGCTACTTTTGTTCCTCTTGTTGCTTCTCTTACAAGATCTTATCTCACCCCAGCTTTGTTCCTAAGCTGAAATCTTTTATTTTATTTTACAAGATTCTCTATTTTTTTCTCATATAATGGTGACCCTTAATATTATTTACATATAATGGATTCCAATACCCAACAGAACCAACCCAATTCTGGGTTGCTTCGTTTTCGTTCTGCACCAAGTTCACTCTTTTCAAATCTCACACCTTCTTTTGTTGATGATAACAAAGGCACTAAAGATTTTTGGGATGATTCTGAATCAGAGAGATTGGTTTCAAGGTTTGTGAGTAACGATAATATTAATATGTCTGGTTCTTCTTGTTCTAAGGAGATGAGTGGAATGAATTCTGGTTATGGCGGCGGCGGCGGCTGTGGTAGTGGGTTGCCACCTCACTATCCTAGACACGGTTCTGCTGCAGCTTCAAGTTCTGCTATGGATGGTTCTTTTGGATTGGTCGGTTCATTGGGGATGAATAATGAAACATCTCATAAGACCTTTGGTTCAAATCTTCTTAGGCAGGGTAGTTCCCCTGCTGGTCTTTTCTCCAACATTTCATTTCAAAATGGGTTTGCTGCCATGAAGGGTGTGGGAA
TARGET=511,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2976
PRIMER_LEFT_PENALTY=0.054900
PRIMER_RIGHT_PENALTY=0.242742
PRIMER_LEFT_SEQUENCE=AATGGATTCCAATACCCAACAG
PRIMER_RIGHT_SEQUENCE=TGTCTAGGATAGTGAGGTGGCA
PRIMER_LEFT=272,22
PRIMER_RIGHT=559,22
PRIMER_LEFT_TM=59.945
PRIMER_RIGHT_TM=59.757
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.3992
PRIMER_LEFT_1_PENALTY=0.156479
PRIMER_RIGHT_1_PENALTY=0.242742
PRIMER_LEFT_1_SEQUENCE=GGCACTAAAGATTTTTGGGATG
PRIMER_RIGHT_1_SEQUENCE=TGTCTAGGATAGTGAGGTGGCA
PRIMER_LEFT_1=384,22
PRIMER_RIGHT_1=559,22
PRIMER_LEFT_1_TM=59.844
PRIMER_RIGHT_1_TM=59.757
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=0.5022
PRIMER_LEFT_2_PENALTY=0.156479
PRIMER_RIGHT_2_PENALTY=0.345718
PRIMER_LEFT_2_SEQUENCE=GGCACTAAAGATTTTTGGGATG
PRIMER_RIGHT_2_SEQUENCE=TCCATAGCAGAACTTGAAGCTG
PRIMER_LEFT_2=384,22
PRIMER_RIGHT_2=592,22
PRIMER_LEFT_2_TM=59.844
PRIMER_RIGHT_2_TM=59.654
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.6536
PRIMER_LEFT_3_PENALTY=0.054900
PRIMER_RIGHT_3_PENALTY=0.598673
PRIMER_LEFT_3_SEQUENCE=AATGGATTCCAATACCCAACAG
PRIMER_RIGHT_3_SEQUENCE=CAGCAGAACCGTGTCTAGGATA
PRIMER_LEFT_3=272,22
PRIMER_RIGHT_3=570,22
PRIMER_LEFT_3_TM=59.945
PRIMER_RIGHT_3_TM=59.401
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.6664
PRIMER_LEFT_4_PENALTY=0.423703
PRIMER_RIGHT_4_PENALTY=0.242742
PRIMER_LEFT_4_SEQUENCE=GATTCCAATACCCAACAGAACC
PRIMER_RIGHT_4_SEQUENCE=TGTCTAGGATAGTGAGGTGGCA
PRIMER_LEFT_4=276,22
PRIMER_RIGHT_4=559,22
PRIMER_LEFT_4_TM=59.576
PRIMER_RIGHT_4_TM=59.757
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_01604 Ps_contig_mira-and-tgicl-ass_franssen_15221
SEQUENCE=CCAAAACAAAATCATGGTACAATAACATAAATTTTGGACACTACAYTCATATAAAACAAATGAATTGCAACCGTCTGTATTTACTAGTACCAGTTTCATGATTCCAACTACAAGTTTTCACCATCATCACTACTGTCAACTGAAGATAAACTACAAAATTTTGAGTGGATAAAGAACATGCCAAATCTTCCCGGGGCTCTAAGGAGGTAACGTTCTATATCGTTTAACATCTTGTCCTTCATGAACACCCAACCCAACGGTAAATCATTAACCGCCTAAACTGGACAACTAAAGAAGTTTCAGTCCACCTGTTACCTCGTCTACCACTTCAACAGGTCCGAGAATCGCCATCACTGTTCTGGAATTCGGTGCAATCTGTGTTCGTCCCGCATCAATTGTGATATGAGTAGGTAACTTCAGAGATTTAGCCCTTTCTTGCAAAGCCAGCATATCTTCTTCACTTTCAATTTTGACAACAACCTTGGGCTGCGCAGACATCTCCCACCTATTTAAAGCCTTTGGAGCTCGATAAAGAACCTTTTTGTAGAGACCCAACGTTGCATGACTGCATTGAGCTGCAATTTTTCCTTTACCCATTTTAAGATCATTTCGAACAACGAGTATCATCTTAAAATCCTCGAGAATTTCCGCGAGCTGTTCAACCTCAAGTGAGTCTTTGATTTTGGACTTACTATTCTTCTTCTGCTTACTGTTATTGTTGTGATTGAGAAGTGAATCATCTTTCTCTTTAAGGGATGAAAATCTATTCGAGAAAAAGAAACGATGGGGATAGTGAGAACCAATGAAGTAGCCGAAAGCGAGATAACCGGCGCCGACCAAAATTGCACTCAACCATGATACTTCCATTTCAATCACTTGAGAAGATGAAGATCACGGTTGGGATTTTAGGGTTCTGAAAAGGGGATTTTAGTAATTAATAAAATARAGTAAAAACACACTTTACTTTCGGCAACAACAACAACACCGAATGAATTAACTCC
TARGET=971,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01605 Ps_contig_mira-and-tgicl-ass_franssen_15225
SEQUENCE=AAACCAAACGAAATMYTTTCTACTATCGTCCGCGTCGATTCTCAGTGAGATCTCAACTTTTATTTTTACTTCATCAATGAGTTACCGAAGAGACAATAGGTCGTCCAGATCGTCACTTTTAGATGGTCTTGATAGTCTAGAGGAGGGTGGTTTAAGGGCTTCTTCTTCTTACTCACCTGAAATAAAAGAGCACGATAATGATACAGCCATGGATACTCTCCACGACAGAGTTTCTTTTCTAAAGCAATTAACAGGTGATATACATGAGGAGGTCGAGAGTCATAACAGCATGCTCGATCGGGTGGGTAACAAAATGGATGGATCAAGGGGTATGATGTTGGGAACCATGGATAGATTCAAGAAGGTTTTTGAGAAGAAATCGACAAGGAAAACATGCTCACTTGTGGGATATTTCACAGTTGGTTTCATATTCGTATACTATCTTATTAGGATGCTTGGATATTTTACACTAGGATGAAGCCTGTTATGTTAAGCTAGCTAGAATGTGGGATGGATGTTTCAAGTAAAATTTTGTTCTTGTGCTATAAAATGCTTGAACCATTGGCTTCTGGTTTGAAAACTCAGTTGTGAATTGTGATCTTCGAAACTTACTGCATATATAGAAAGCTTAAACAAAAGTATATTGTTTAATTACCTACTAAACT
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01606 Ps_contig_mira-and-tgicl-ass_franssen_15226
SEQUENCE=AGAAAAATGGTGGGAAAAAATTAAAGAAGAAGCTTTAAGTACACATATGAATGCATGTGTTAAGTTTAACAAATATGGAAGGGCTTTTCCTGTTTTGTTTCAAGCTCAAGCTATGAAGGAAATGCTGAAAAAGAGTAACTTAGATGGGAAAATTAGGGCAAAGTCTATTAGTCCAAGGGACAAGAAAGGAGGAGGAGGACTTACACGTAGTTCAAGCTGTATTCCAGCACTTACAAGAGATATACAAAAATATCAAAATTTAAATCTATCTATCTTAAGAAAAATCATATGATATTAATGTGTGACAATATTTTCATGTTCTTTGAGCACATGAACTTTACTCGTGAGTTATGATCTTACTATAAGATAGTTTTTTTATAGGCCTTGTTAGAAGTTGCCTTAGGTTTTTTTAGGGTATTGGTAACCCTTTTACTTCTCTAGGCTGCTTCTTTAATTTTGGTCTATGTAGATTTTTTTAAAATAACTTTGAGCAATGTATTGTAGATMTTACCAATTATATTTTGGAATAAAATTCAA
TARGET=187,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01607 Ps_contig_mira-and-tgicl-ass_franssen_15226
SEQUENCE=AGAAAAATGGTGGGAAAAAATTAAAGAAGAAGCTTTAAGTACACATATGAATGCATGTGTTAAGTTTAACAAATATGGAAGGGCTTTTCCTGTTTTGTTTCAAGCTCAAGCTATGAAGGAAATGCTGAAAAAGAGTAACTTAGATGGGAAAATTAGGGCAAAGTCTATTAGTCCAAGGGACAAGAAAGGAGGAGGAGGACTTACACGTAGTTCAAGCTGTATTCCAGCACTTACAAGAGATATACAAAAATATCAAAATTTAAATCTATCTATCTTAAGAAAAATCATATGATATTAATGTGTGACAATATTTTCATGTTCTTTGAGCACATGAACTTTACTCGTGAGTTATGATCTTACTATAAGATAGTTTTTTTATAGGCCTTGTTAGAAGTTGCCTTAGGTTTTTTTAGGGTATTGGTAACCCTTTTACTTCTCTAGGCTGCTTCTTTAATTTTGGTCTATGTAGATTTTTTTAAAATAACTTTGAGCAATGTATTGTAGATMTTACCAATTATATTTTGGAATAAAATTCAA
TARGET=264,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01608 Ps_contig_mira-and-tgicl-ass_franssen_15245
SEQUENCE=ACGATATTCCTTATTTTAGACCTAACATGGATGAAACTGTACGCTCACAGGTTCTTTCACCACATCAGGAGGTTTTGAACTCCCATGCTGCAAACAATGCTCCCACATCCACAATTGCCATGGTGCAACAACAGCAACAACAACAACAACAACATCAAGTTGCACAGATGTATCCANAAGTTCATCTTTCACATTACGCTAATCTTANGCCCTATCGTCAGTTTTCATCCT
TARGET=135,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3054
PRIMER_LEFT_PENALTY=0.012470
PRIMER_RIGHT_PENALTY=0.292894
PRIMER_LEFT_SEQUENCE=TCACAGGTTCTTTCACCACATC
PRIMER_RIGHT_SEQUENCE=CGATAGGGCNTAAGATTAGCGT
PRIMER_LEFT=44,22
PRIMER_RIGHT=216,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.293
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.50
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.75
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.3054
PRIMER_LEFT_1_PENALTY=0.012470
PRIMER_RIGHT_1_PENALTY=0.292894
PRIMER_LEFT_1_SEQUENCE=TCACAGGTTCTTTCACCACATC
PRIMER_RIGHT_1_SEQUENCE=ACGATAGGGCNTAAGATTAGCG
PRIMER_LEFT_1=44,22
PRIMER_RIGHT_1=217,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=60.293
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.50
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=9.2000
PRIMER_PAIR_1_COMPL_ANY=4.75
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=174
PRIMER_PAIR_PENALTY_2=0.5303
PRIMER_LEFT_2_PENALTY=0.012470
PRIMER_RIGHT_2_PENALTY=0.517877
PRIMER_LEFT_2_SEQUENCE=TCACAGGTTCTTTCACCACATC
PRIMER_RIGHT_2_SEQUENCE=AGGATGAAAACTGACGATAGGG
PRIMER_LEFT_2=44,22
PRIMER_RIGHT_2=230,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=59.482
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=0.5539
PRIMER_LEFT_3_PENALTY=0.012470
PRIMER_RIGHT_3_PENALTY=0.541428
PRIMER_LEFT_3_SEQUENCE=TCACAGGTTCTTTCACCACATC
PRIMER_RIGHT_3_SEQUENCE=ATGAAAACTGACGATAGGGCNT
PRIMER_LEFT_3=44,22
PRIMER_RIGHT_3=227,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=60.541
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.50
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.50
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=10.3250
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.5608
PRIMER_LEFT_4_PENALTY=0.267873
PRIMER_RIGHT_4_PENALTY=0.292894
PRIMER_LEFT_4_SEQUENCE=ACCACATCAGGAGGTTTTGAAC
PRIMER_RIGHT_4_SEQUENCE=CGATAGGGCNTAAGATTAGCGT
PRIMER_LEFT_4=58,22
PRIMER_RIGHT_4=216,22
PRIMER_LEFT_4_TM=60.268
PRIMER_RIGHT_4_TM=60.293
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.50
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_01609 Ps_contig_mira-and-tgicl-ass_franssen_15261
SEQUENCE=TGATGAATGATGTAGCTGAGGAGGACAGAGGGGGATTGATTGAGCTACACAGCTACTGCACCAGCGTATACGAACAGGGCGATGCTCGCTCTGCTCTTATTGCTATGGTGCAGTCTATAAACCATGCAAAAAATGGTGTGGACATTGTGTCAGGCACTCGCGTCAAGTCTCACTTTGCCAAACCCAATTGGCGATCTGTTTACAAACGCATTGCACTTAATCATCCGGAAGCAAGAGTTGGGGTATTTTACTGTGGGCCATCCACCCTGACCCACGAGCTTCGCCAGCTATCGTTGGATTTCTCTCATAATACATCCACGAAATACGACTTTCATAAAGAAAATTTCTGATAAGATGAAAATAATTGAGGATTACGGTGAGAGCTAGAACTCCACCAAGATGACTAAACAATTATCATGTATTGTCTCCACCTTTCTGTCGATAACCTAAAGTGTGTTTCTACTTTCTACCTATGGTTGGGATGGTTTTAGTTAAGGATCAAGATATACGTGGATGGTTTGGTTGTGAGTTGGCTACCAAATACTAAAGGTGTGGTAAGAATAGGATGGCCCCGTCAATAATTTATAAGCGTGTCGTCATGATTGTAAATTTAATACATTAAAGGGAAATAGATGACCACGACAAGGATTTTTTTGTTTTGTTTTGTTTTTCATTCATATTGTGATCTCAAGATAAGGGACCAGGAAGAAAGTAAACGAGAAAGGAAATAATAAGATGCAGTGCAATYAAWARTTCATGTTTTTAATTGTATTTTGTTAATTTATTTTATTTTATT
TARGET=652,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01610 Ps_contig_mira-and-tgicl-ass_franssen_15261
SEQUENCE=TGATGAATGATGTAGCTGAGGAGGACAGAGGGGGATTGATTGAGCTACACAGCTACTGCACCAGCGTATACGAACAGGGCGATGCTCGCTCTGCTCTTATTGCTATGGTGCAGTCTATAAACCATGCAAAAAATGGTGTGGACATTGTGTCAGGCACTCGCGTCAAGTCTCACTTTGCCAAACCCAATTGGCGATCTGTTTACAAACGCATTGCACTTAATCATCCGGAAGCAAGAGTTGGGGTATTTTACTGTGGGCCATCCACCCTGACCCACGAGCTTCGCCAGCTATCGTTGGATTTCTCTCATAATACATCCACGAAATACGACTTTCATAAAGAAAATTTCTGATAAGATGAAAATAATTGAGGATTACGGTGAGAGCTAGAACTCCACCAAGATGACTAAACAATTATCATGTATTGTCTCCACCTTTCTGTCGATAACCTAAAGTGTGTTTCTACTTTCTACCTATGGTTGGGATGGTTTTAGTTAAGGATCAAGATATACGTGGATGGTTTGGTTGTGAGTTGGCTACCAAATACTAAAGGTGTGGTAAGAATAGGATGGCCCCGTCAATAATTTATAAGCGTGTCGTCATGATTGTAAATTTAATACATTAAAGGGAAATAGATGACCACGACAAGGATTTTTTTGTTTTGTTTTGTTTTTCATTCATATTGTGATCTCAAGATAAGGGACCAGGAAGAAAGTAAACGAGAAAGGAAATAATAAGATGCAGTGCAATYAAWARTTCATGTTTTTAATTGTATTTTGTTAATTTATTTTATTTTATT
TARGET=781,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01611 Ps_contig_mira-and-tgicl-ass_franssen_15262
SEQUENCE=CACAAATAATCTATATTTCTTTGCAGTKCWKYATTATATATCTTTTTCTTTTTCTTCTTCTTCCATAGATTTAAAGCCTAACAATCTCAGGAATATGAACAGGATAACGATCAATGCAATCTTCCCAAGGTCCATCAATGGGTGTCTTAATGCTTCTGTTACATTTCCACACAGCACTATTGCACTTAAATCCACTTCCAAAAGCTATTTGCCATATTCTATCTCCTTTCTTCATCCTTCCTTTTGCTTCAATATAGTTTAATTCATACCATAAAGACGAAGAAGACGTGTTCCCGAATCGGTGCAATGTCATCCTCGAAGCCTCGACGTGTTCCGATGATAGCTGAAGATTCTTCTGTAACTCATCGATCACGGCGCGGCCACCAGCATGAATACAAAAGTGCTCAAAGGCTTGTTTGAAATCAGGAATATAAGGCTTCCATTTAGGGTTGAAAACTTTCTTTCCAATGAGTGTTAAAAGAAAGAGTAACTGTTCTGAAGCTGGTAGAACAAGAGGGCCAATAGTTGTTATATTTGATTTCAATGCTTCTCCTGCTATAGCCATGAGATCTTTTGAAAGTGAAATTCCTACTTTACCTTCTCGGTCTTCTTCTTCGAAAACGCAACGGTAAGCTTTATCGTCTGCGCCTTTGTGTGTTCTAACAACTTGGAGTAATCTATATTTAGCTCTTTTGTGTTCCTTTTTTTTGTTTGATAAAAGAATTGCAGCACCACCCATTCTGAATAAACAATTTGGTAAGAGCATTGCTCTTTCTTTTCCTTGGTAATAGTT
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01612 Ps_contig_mira-and-tgicl-ass_franssen_15270
SEQUENCE=TTTGATGTTTGAATCGTTGATGGTTGTTGATTTTTGAATGAATTATGGATGGTTTGAGTTTTGTGAGATTAGGGTTTTGGGTGGTCTGATCCCGTCGTTTGGCTTGGATTTAGGTGAAATAGAAGTCAGATTTGAAAAATTGAAGGTAAAAAGAGGAAGGGGGTTTATGTTTAGGTTTTTGTGGTTGGTTGTGGTTGGCCACCAAAGAGGACGGTGGCACCACCGCCCCTCTCCAGTGAGGTTAGATGAGGAAGATGACCGGAACGGTGCAAAGTTTATTCTTGAAGAGAGAGAGAGAGAGGAGAGAGAGAGAGAAGTAATANAATATACTTCCTCCCTCCCGT
TARGET=286,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01613 Ps_contig_mira-and-tgicl-ass_franssen_15270
SEQUENCE=TTTGATGTTTGAATCGTTGATGGTTGTTGATTTTTGAATGAATTATGGATGGTTTGAGTTTTGTGAGATTAGGGTTTTGGGTGGTCTGATCCCGTCGTTTGGCTTGGATTTAGGTGAAATAGAAGTCAGATTTGAAAAATTGAAGGTAAAAAGAGGAAGGGGGTTTATGTTTAGGTTTTTGTGGTTGGTTGTGGTTGGCCACCAAAGAGGACGGTGGCACCACCGCCCCTCTCCAGTGAGGTTAGATGAGGAAGATGACCGGAACGGTGCAAAGTTTATTCTTGAAGAGAGAGAGAGAGAGGAGAGAGAGAGAGAAGTAATANAATATACTTCCTCCCTCCCGT
TARGET=302,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01614 Ps_contig_mira-and-tgicl-ass_franssen_15294
SEQUENCE=CCTATCCCCTTTTGCGTGTCTCAGTAATTAATTAATTATGGTATTTTATAATTTATGTTAGTAGTGTTTTACTAAAATTGGAAGAGGTGGAAATTAATACCACCCGCCATATTAGTATTGTTGGAAGCATTGTTTGCTTCACTAATTTATTGTGAGCAACTTTAGCTGAGTATGGTTGGGTTTGGTGTATTAAGAGAATGTAATTTAGTTAAATTTTTCTTTATTTATGACAAAGTAATAGGTAATTTATTATTTAATTTGTTTAGTTTTTAAACTTTTAAAAAAAAGG
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0014
PRIMER_LEFT_PENALTY=1.991339
PRIMER_RIGHT_PENALTY=0.010077
PRIMER_LEFT_SEQUENCE=CTATCCCCTTTTGCGTGTCTC
PRIMER_RIGHT_SEQUENCE=CAATACTAATATGGCGGGTGGT
PRIMER_LEFT=1,21
PRIMER_RIGHT=119,22
PRIMER_LEFT_TM=60.991
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=2.0014
PRIMER_LEFT_1_PENALTY=1.991339
PRIMER_RIGHT_1_PENALTY=0.010077
PRIMER_LEFT_1_SEQUENCE=CTATCCCCTTTTGCGTGTCTC
PRIMER_RIGHT_1_SEQUENCE=ACAATACTAATATGGCGGGTGG
PRIMER_LEFT_1=1,21
PRIMER_RIGHT_1=120,22
PRIMER_LEFT_1_TM=60.991
PRIMER_RIGHT_1_TM=59.990
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=2.0837
PRIMER_LEFT_2_PENALTY=2.073614
PRIMER_RIGHT_2_PENALTY=0.010077
PRIMER_LEFT_2_SEQUENCE=TATCCCCTTTTGCGTGTCTC
PRIMER_RIGHT_2_SEQUENCE=CAATACTAATATGGCGGGTGGT
PRIMER_LEFT_2=2,20
PRIMER_RIGHT_2=119,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.990
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=2.0837
PRIMER_LEFT_3_PENALTY=2.073614
PRIMER_RIGHT_3_PENALTY=0.010077
PRIMER_LEFT_3_SEQUENCE=TATCCCCTTTTGCGTGTCTC
PRIMER_RIGHT_3_SEQUENCE=ACAATACTAATATGGCGGGTGG
PRIMER_LEFT_3=2,20
PRIMER_RIGHT_3=120,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.990
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=2.8156
PRIMER_LEFT_4_PENALTY=2.805475
PRIMER_RIGHT_4_PENALTY=0.010077
PRIMER_LEFT_4_SEQUENCE=CTATCCCCTTTTGCGTGTCT
PRIMER_RIGHT_4_SEQUENCE=CAATACTAATATGGCGGGTGGT
PRIMER_LEFT_4=1,20
PRIMER_RIGHT_4=119,22
PRIMER_LEFT_4_TM=59.195
PRIMER_RIGHT_4_TM=59.990
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_01615 Ps_contig_mira-and-tgicl-ass_franssen_15295
SEQUENCE=TTTTCTTTTTGAATTAATCTTCTGTTGCTAAAAAAGCTTACAGAGATTAATTAATTAATTATGGTATTTTTATAATTTATGTTAGTAGTGTTTTACTAAATTGGAAGAGGTGGAAATTAATACCACCCGCCATATTAGTATTGTTGGAAGCATTGTTTGCTTCACTAATTTATTGTGAGCAACTTTAGCTGAGTATGTTGGGTTTTGGTGTATTAAGAGAATGTAATTGTAAATTTTCTTATTTATGCAAGTAATGGAAATTTATATAATTTGTGTA
TARGET=46,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01616 Ps_contig_mira-and-tgicl-ass_franssen_15319
SEQUENCE=AAAACAGAGTATCGATAAATAAAATGCAGAAATGAAATTCGTGAAGAACGTGGAATGCCAGCGAATAGAATTGCTTTGTATAATGGAATTTTAAGAGATAGAGTTCATAGAATCGGGTCATGTCACTGATCATCATCATCATTATTCCTTTTAGGAAATTAGAAGTTAGTAATTTTCAGGGTATGTAATTGCTCTTGTTTTTCTTTTTATTTTATTTAGAAGAAGTGTTGGAACATATTTGAAAGTTCAAAACGGG
TARGET=129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01617 Ps_contig_mira-and-tgicl-ass_franssen_15327
SEQUENCE=AGTACGCAGGGRAAGCATCAGACTCAAGGGATATGTGAAAACATGAGATCTATTTAATGCTTGTAATAAGAATATTGTGGGGATGTGAGAATTTTGCTTTTTTTCTCTCTCTCAACGCTTGTATAAACTTTGGTTAAGTTGTTATTCAATACATATCTCCCTTCATTTTTTCCATTGTTGTACGGCATTGTTTCACTATTGCTTATATTTCACAATGAACAAGGAGAAGTAGTAG
TARGET=104,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01618 Ps_contig_mira-and-tgicl-ass_franssen_15371
SEQUENCE=GGTTGGTTAGAATAAGGTGATTATTATTATTATTGTTGTTGGGAAAAGGTGATCATCGGAAGCTCTTTACAACACTTCCCGTCACCTCTTCAATTCTTTTCCTCTCTCCAATCCTTTCAGACATGTCAAGTGGATCTGTGAGACGGGTTTCTCGGGAAGATATCCAGCTGGTACAGAATCTCATAGAACGATGTCTGCAACTATATATGAACCCAAAAGAAGTTGTGGAAACTCTACTAGCTCAAGCAAAAATTGAGCCTGGTTTTACCGAACTTGTTTGGCAAAAGCTTGAAGAAGAAAATCAAGAATTTTTCAAGGCTTATTATTTGAGGTTGCTGCTGAAGGAGCAAATAACGGAGTTCAACAGGTTGCTGAAGGAACAAGCTGAGTTAAGTCAGCTACAATCAACTGCTGTTGCTTCACTACCCAACTCTAATGGTTTACATATTTCTTCATTACCTCAGAATCCATCTTGCTATGCCTCTGAGCAAGCCAGTGCTGCTGTGAAGCCAGAGAATATGAGACATGCTCTTGATTCCACGTTACATGATGTATTTAACAATGGCGGGTCATCACTGAACACAAGTATACATGATTTG
TARGET=20,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01619 Ps_contig_mira-and-tgicl-ass_franssen_15372
SEQUENCE=AGTCAGCTACAATCAACTGCTGTTTTTGTCTTCACTACCCACCTCTAATGGTTTACATATTTCTTCATTACCTCAGAATCCATCTTGCTATGCCTCTGAGCAAGCCAGTGCTGCTGTGAAGCCAGAGAATATGAGACATGCTCTTGATTCCACGTTACATGATGTATTTAACAATGGCGGGTCATCACTGAACACAAGTATACATGATTTGACTCAGATATCTGCTCGTGGAAATAGGATTAGCAGCCCACCAAGCATGCTTTCATCTCAAAACTCAAGCTTGGGTTTGATACAAGGAGTTAATGGGGGAATGATAAAATCAGAACCCGGATATTCGGGTAGTCCTCCTTACATCTTTGGTCATGATGGCTCTGTGTTGGAAGCATGCCCAACTATTGCTGATGCAGCGGTTACATCGTTTAATAGTGTTGACTCAAACTCTCATTCTATGAATGGAGCACTCATTGACCCAGATATTTCATCATTTGGAGTTTTGGGGCAGATTTCTAGAAACCTCAGCCTTTCAGATCTGACTGCTGATTTTTCTCAGAGTTCTGATATAATGGAGAGCTATCCAAGATGTCCCTACCTGGGTACTACTAATGAGAACTTCCTTCAAAATGGAGAACAGAATTGAACAGCACCCGTAAAATGAAGTGCTTGTAAATAGAAGTTTAGAAGTTTGACAGACTATGAAATTCCAACTCTTCCGTGAACATGTGTATAGGAGTTCACTAACCATAATCTGCAGTGATGGGAAGTTAAATTTGAAAGGGTTTGTATGTACTCAATAAATAATGAATCTCTCTTATTTTGGTTTGTTTTCATCCTGTGTCCGTTTGGGTATAGCTTTGCATAGTGGTTTTTTCTACTTTGTTTATATATATACTAGTTCTCTTGTGTTTTGATGATGTTATTTTTGGCGTAGAATGCATGAAACTGTGTTTTACAGGGATGATGTATCTAGAGTTTTCACCTTATCAAATGAAAAACACTACTGTTATAAGTTACWAAAAGTT
TARGET=879,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01620 Ps_contig_mira-and-tgicl-ass_franssen_15375
SEQUENCE=CCTCTCTAGGTTTTCCTTGAAAGAGGTTGAGGTAGATACCAGAATGGGATTTTCCAACAAAGAAAGTAGAATTTCCAGTCCCTGAGATGTTGAGGATGAATGGGTAGATAATGGAACCCCGTCAATTAATATCATGATCAGTTAGGATTGGAAATAAGCTTCACAAGTAGGGTAGCAGTTTCTTCTTCTTCTTCAATTTGAACGATCGCACTAAACCATAGAGAAATCTTGGACTTGTTCASVHHGTMAAAAAAAAATC
TARGET=180,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01621 Ps_contig_mira-and-tgicl-ass_franssen_15376
SEQUENCE=ATACGCGGGGACATCTGAACAAGTCCAAGACTTCTCGATGGTTTAGGGCGATCGTTCAAATTGAAGAAGAAGAAGAAGAAGAAACTGCTACCCTACTTGTGAAGCTTATTTCCAATCCTAACTGATCATGGTATTAATTGACGGGGTTCCATTCTCTATCCACTCATCCTCAACATCTCAGGGTCTGGAAATTCTACTTTCTTTGTTGGAAAATCCCATTCTGGTATCTACCTCAACCTCTTTCAAGGAAAACCTAGAGAGGAAGTTTTCAGTCTCTGAGGTAGTTTTCTCCAGAGAGATCAAAATTGGTTTATTTATTCCAAAAAGAATATGCTACTGTTG
TARGET=63,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4334
PRIMER_LEFT_PENALTY=0.378922
PRIMER_RIGHT_PENALTY=0.054514
PRIMER_LEFT_SEQUENCE=GTCCAAGACTTCTCGATGGTTT
PRIMER_RIGHT_SEQUENCE=TACCAGAATGGGATTTTCCAAC
PRIMER_LEFT=22,22
PRIMER_RIGHT=225,22
PRIMER_LEFT_TM=59.621
PRIMER_RIGHT_TM=60.055
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=0.6434
PRIMER_LEFT_1_PENALTY=0.378922
PRIMER_RIGHT_1_PENALTY=0.264444
PRIMER_LEFT_1_SEQUENCE=GTCCAAGACTTCTCGATGGTTT
PRIMER_RIGHT_1_SEQUENCE=TTTTCCTTGAAAGAGGTTGAGG
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=251,22
PRIMER_LEFT_1_TM=59.621
PRIMER_RIGHT_1_TM=59.736
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.6578
PRIMER_LEFT_2_PENALTY=0.378922
PRIMER_RIGHT_2_PENALTY=0.278847
PRIMER_LEFT_2_SEQUENCE=GTCCAAGACTTCTCGATGGTTT
PRIMER_RIGHT_2_SEQUENCE=CTGAGATGTTGAGGATGAGTGG
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=180,22
PRIMER_LEFT_2_TM=59.621
PRIMER_RIGHT_2_TM=59.721
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.6578
PRIMER_LEFT_3_PENALTY=0.378922
PRIMER_RIGHT_3_PENALTY=0.278847
PRIMER_LEFT_3_SEQUENCE=GTCCAAGACTTCTCGATGGTTT
PRIMER_RIGHT_3_SEQUENCE=CCTGAGATGTTGAGGATGAGTG
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=181,22
PRIMER_LEFT_3_TM=59.621
PRIMER_RIGHT_3_TM=59.721
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=0.7691
PRIMER_LEFT_4_PENALTY=0.378922
PRIMER_RIGHT_4_PENALTY=0.390220
PRIMER_LEFT_4_SEQUENCE=GTCCAAGACTTCTCGATGGTTT
PRIMER_RIGHT_4_SEQUENCE=GGTTTTCCTTGAAAGAGGTTGA
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=253,22
PRIMER_LEFT_4_TM=59.621
PRIMER_RIGHT_4_TM=59.610
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_01622 Ps_contig_mira-and-tgicl-ass_franssen_15378
SEQUENCE=ACGGGGGATTCCAATAACAAAAAATAAAATCTCTGGATTGGATCCCGAATTGCTCAGATTTCAACATCTCTTTCGTTTCACACTCACAAGTCTTCACAGCTCAACTCAACTAACCAAAAAAAGTTTAAATTTTCGCTTCAAATTCCCATAACCATCTCACTCTTCTGCTTCTATCTATCTTCCATTATCTTCATTTTCAAGATCCATTCACTGATTCACCAAAATTTCTCTTCTGTTTCCGTTACAAAGAAAGAAAGGGTAACTTCTTCGAAATCAACAAACTAGGTTTTCGTATGAACTGATTCTGTTTTTCTTCTGCGATTAGAGTTTTTGAACTTCGATTGTGTTCTGTATATTTGCTGAAGTTGGTTGTAGTTCTGTAATCATGGAAGAAGAGAATGCTATAGATCTTCTTCAAAGATATAGAAGGGATAGGCGAGTACTTCTCGATTTTATACTTTCGGGGAGTTTGATTAAGAAAGTTGTGATACCACCTGGTGCTGTCACGCTCGATGATGTGGATCTAGATCAAGTCAGTGTTGATTATGTTCTTAATTGTGCTAAAAAGAGTGAAATGCTTGAGCTTTCAGAAGCTATTAGAGAATATCATGACCACACCGGGTTACCCCAAATGAGTGACACAGGTTCAGTTGGTGAATTTTATTTGGTCACTGATCCCGAGTCTTCAGGTTCACCTCCCAAAAGGCCACCCCCACCTGTTCCTATAACTGCAGTGCCTCCTATCGCTGTTTCCACTCCGCCTCATGCTTTTCCTCCGTCTTCAATTGCTTCTAATCTATCAAGATCAGAGTCTTTAGACTCCGCACAAGAAAGAGAGTTAACTGTGGATGACATTGAAGACTTTGACGATGACGACGATATTTCTACAGTAGAAGGTCTCAGGGCTAAAAGGACATTAAATGATGCTTCCGATCTTGCTAG
TARGET=246,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5992
PRIMER_LEFT_PENALTY=0.203367
PRIMER_RIGHT_PENALTY=0.395857
PRIMER_LEFT_SEQUENCE=GATCCATTCACTGATTCACCAA
PRIMER_RIGHT_SEQUENCE=TCTTAATCAAACTCCCCGAAAG
PRIMER_LEFT=200,22
PRIMER_RIGHT=478,22
PRIMER_LEFT_TM=59.797
PRIMER_RIGHT_TM=59.604
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.5992
PRIMER_LEFT_1_PENALTY=0.203367
PRIMER_RIGHT_1_PENALTY=0.395857
PRIMER_LEFT_1_SEQUENCE=GATCCATTCACTGATTCACCAA
PRIMER_RIGHT_1_SEQUENCE=CTTTCTTAATCAAACTCCCCGA
PRIMER_LEFT_1=200,22
PRIMER_RIGHT_1=481,22
PRIMER_LEFT_1_TM=59.797
PRIMER_RIGHT_1_TM=59.604
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=0.7249
PRIMER_LEFT_2_PENALTY=0.203367
PRIMER_RIGHT_2_PENALTY=0.521503
PRIMER_LEFT_2_SEQUENCE=GATCCATTCACTGATTCACCAA
PRIMER_RIGHT_2_SEQUENCE=CCCCGAAAGTATAAAATCGAGA
PRIMER_LEFT_2=200,22
PRIMER_RIGHT_2=465,22
PRIMER_LEFT_2_TM=59.797
PRIMER_RIGHT_2_TM=59.478
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.9858
PRIMER_LEFT_3_PENALTY=0.589903
PRIMER_RIGHT_3_PENALTY=0.395857
PRIMER_LEFT_3_SEQUENCE=AGATCCATTCACTGATTCACCA
PRIMER_RIGHT_3_SEQUENCE=TCTTAATCAAACTCCCCGAAAG
PRIMER_LEFT_3=199,22
PRIMER_RIGHT_3=478,22
PRIMER_LEFT_3_TM=59.410
PRIMER_RIGHT_3_TM=59.604
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.9858
PRIMER_LEFT_4_PENALTY=0.589903
PRIMER_RIGHT_4_PENALTY=0.395857
PRIMER_LEFT_4_SEQUENCE=AGATCCATTCACTGATTCACCA
PRIMER_RIGHT_4_SEQUENCE=CTTTCTTAATCAAACTCCCCGA
PRIMER_LEFT_4=199,22
PRIMER_RIGHT_4=481,22
PRIMER_LEFT_4_TM=59.410
PRIMER_RIGHT_4_TM=59.604
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_01623 Ps_contig_mira-and-tgicl-ass_franssen_15399
SEQUENCE=ACTTTTTTTAAAATTTACTTATTATCATAYATTGGTGAATTCAAATTTATCATAAATACAAACTAGAAGATATTCATTTGAATTGTACAAATAGTACTAATGAAGTACATTGTTCTGTCCTAAAAAATATGCAAAACCTTGAGAATCAAACCAAGATATCGAGACAAAAGTATGGGTCTTCCGAAAACTAATAGTTCAATTGCATAATGCATTGAACAAAAGAAAAAGCTCAAGCTGAAGCAAACTCTGACACACAAACCCAAAAAAATGGATGGTTCCACAAGCCACAGAAAAATAACAAGCACAGGCAGCCGAACAAGCATACAGTAAACAGATATATAGATGATTGTAAATGAATCAGAGAAACAAGGAACTGAAGCCACTCCCACAGCAATCCAAGGAAAGATGAAGTAAAAATAATAAGAAACTCTCAATTGCCCGTCAACTTTAACCACCTGAGTGAGAAGCAGGAGTAGTTTCAAAACGTCCTTCCTTGAATAAATGAATGTACTGTTGTGGCAAAGAATGCTCCACCCAATCACCATCAGACTCGGCGCTAGGAACTAATACATTGATCTCACTTGACTTTGCTGTGGATATGGATGTTTCTAAAGAGTCTTTGCTCAGATATAACTGGCATCCAGAAGTGTTGTCCACCGAAATTGTAGGAGCTGAACCCTGGCATTGAACCTCAACCCCATTACTGTTCACAATCTCACAAGCTGCAACAACGTCCTGAAATACAACTCCCATCTTTTTGCAATTGTCAATAGTTATATTGTTGACCTTGCCTTGAATCTGCAACACGGAGTTTTTACATCCATAAACATATACAGACTGTTTTGCATCACAATCTTCAATGACCAAGCTTTTCTGCTCAATTTGATTCTCAACAACCCACTTGCGACCCATTTGAAGTTCGAACTTCGGAGGTCCTACTTTAGCAACTGCACGTGAACCGGCGTGACTTTCTTTTACGGTACTGCCGCCAACAATTCCAGCTCTTTCTGTGCGATTCTTAGTCTTCATATCAGCCGTAACCTTTCTCAGACCTGCTGTGACATTTCCTGTACCAATCTCTTGAAAAACAGCCGACATCCCCACTTTTGGCTTAGAAGATGAAGCCTGAGATGATTCAGAACTAAARGGAGACGNGAGGGCGGAGGGAGGGAGGGGCAAGGTAAGGTGACTAAGTACGGAAA
TARGET=1162,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01624 Ps_contig_mira-and-tgicl-ass_franssen_15406
SEQUENCE=AGGGGTCATCAATSKGGCCGAATAATCGCTGATTTCTCGAACAGCGTGTCGTTTTCCGTTCCCGCCCACTTCCATCTCTCTCTCCACCATGGGAAACTCCTCCAAGGATAATTCCGCCAACTCCGGCGACGCATCTCCCGCCGACGCTCCTCCTTCCGATTCAAGCACATACTCCGAGGGCGAGAAAGTCCTTGCTTATCACGGTCCCCGCATCTACGAAGCAAAGGTGCAAAAGGCAGAGATCAGAAAGAATGAATGGAGATACTTTGTTCACTACCTTGGCTGGAATAAAAATTGGGACGAATGGGTAGGTGAGAGCCGTTTAATGAAACATACTGAAGCGAATGTTGTGAAACAGCATGCCTTAGACGAAAAGCAAAGGTGTAAGACAAGAATGTTGAATCTGGGCGTACT
TARGET=75,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01625 Ps_contig_mira-and-tgicl-ass_franssen_15425
SEQUENCE=CGTAATAAMWMAAACTTTAGTAAATTTTAAAAACAAGTTTTGTCAGCAACACACACATGATTAAATACCAATTAAAAACAATTGTAATAGATACATTTGGAAGAACTCGAAATCGTTACCAAAACACGGATCTTCGTTGCTGCCAATTGCGGAGGCGTTCGCCGATACTGCCGCTGAGCTTGTTGATGACGTGAAAAATGCTGACTCGTACCAGTGTTGAAATCTAGTCGGAGCTAATGGTCGAAAGATTGTCGTTGTAGTGTTGCGTTGATGATGACGAAGGTTTTTCGTGAGGGTTGGTGGCCGGAGGGTTCGACGGTCGTGTGGGGGTTTGGAGGATTGTGTTGAGCGGCGATTTTGACGGAAGATGAAGGTAAAAGGGTTTCGTGGTGGTGGTTGCCGCGGATTCGCCGGTGAATGAAGGATATTTCGGTGAGGGTTTACGGCGGATACGGTAATGAAGGGAGCTGGGTATAGGCGATGAAGGAAGGGTTTGGGTAGGTGACGATGAGGTTGTTGTTGTTGAAGGAGAAGAGGTGGATGGCGGCGGTAGGTTAGTGAAGAGGTTGGTCGCGATTGGGGTTTGTTAGTGAGAGATTTCTTTGTGAGAGAGGGAGAGTGAGGTTAGCGTGAAATGGAAGAGAGGTATTTTAGAGAGTGATTCAGAGTSMGTGAATAGTGTATTTAGTGAATTTTTTTTTGTCTTTTTTCTTTTCCCCCCGCGTACT
TARGET=513,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01626 Ps_contig_mira-and-tgicl-ass_franssen_15457
SEQUENCE=ATANACNCAACWTATTGTGTAGCAGGAGCAGCATGAAAATAGAAATACCCATTTGTAATAAATATACAGAAACTGAAACTCATAAACTGCTAACATATGGATACCAATAAGAAAGGTAACAGAGAAGCCTATAATGAGGAACGACAACTTTATCATGGATTTGAGATGGAAATGCTATGCAAATGGCCAATGAAAGCTGTCAGTTACAGGCCTGCCACGGAAGATCATTTCATTCATTCATTCAATCAGCATTTTGATTAAGAGGATTATCAGGAGATTTCTTCCAAAGCTTCCAAATCATATCCTTGTATTGTGTGTAAGTGAGTCCCGGTTTCTCTTGCTTCAATATCGGAAGTTCTGTTTCTTCAAACGCCTTGAAGGAAGCCTTGAGGCGTTTCTCGGGATGCCTGTCAAGGGGTAAGTTGTTATCGATGCTCATCTTAGCGATGGCTTCGTCAAGAGTACTAGCTTCAATTATCGAATCATCACGATTCGTGTTTGATACAAGCACCACTCTCTCGTACTCCTCCTCCGCGGCCGAGCGATTATTCGCTTCCGCGGCCTTCTTCTGAGCCAGAGCATGCTCCTCCTCGCGCCGCTTCCTTAGCTCCACCTCCGTCACCTTCGGTACAGGGATCGACACCCTGTTCGCCTTCTTGTCGGGCTTTTTAATCATTTTCTCGAGTTCTTTCTCCTCCAGCTCCGCCAATCGACGTACCTCCGCTCGCTTGGCCGCCGCCTCGGCCTTCTTATCCGCCTCTTCCTCCCTCTTCTTGGCGGCGCGTGGCTTTGAACTCTCAGCCTCTCGCCAGTATTGCTCCTCCTTCTCTCGAGTCTCCCGGTCCTTTTTCTCCGTTTCAGCGGCGCTTTTGCGAGCCCTTGCCACCTCGGCCTTAGAATTGACACCCATCTTTTTCGGCATCTTAACGCCTAGTGTAGTAGGGTTTGGGTGGTGAATTGGTATTTTCTTCTGCTTCTGCAACTGCACTTACAATTACGATTACAAGCAGTCGAAATGGAAGAAAGAMACTC
TARGET=229,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01627 Ps_contig_mira-and-tgicl-ass_franssen_15465
SEQUENCE=TACGATAAATACTTACCGGCAGAACTAGTAATTTTATTGAAATCATCTCACATTCTTTTACAATAGTATATTGTGTACTTAACAATTAACACAACATATAGATTCATACATACATGCATCATCTATATGATATCAATCTGGGAATTACATTCTCCAAACCACTCAAATTAAACTGCAATTAGATGAACTCAATACTAATAAATACACATAAACAACCCAAGAGCATAATTAATTATAGGGTAAAAGCTAAGCAGAATGAATATTTCCATAATGCTTGTATGCTTATTATTATATAGTTATATTATATACACATATCTTCAGCCAAAGAAATGGTGTTTTTTCTTTCCATGGGCTTCTTCATCTTCCTCCTTGGATTCTTTTTTCTCATGATGTTCATGAAAGGCAAATCCACCAGATCCCACTGCAGCAGCTGCTGCAACCTCTTCTTCTATCTTGTGCCTGTGAGCGTTCTCAGGATCCTTCCCTGATTTGTGCTTCTCATACAAAGCATAACCACCGGCAGCTACAGCACCAAACTCACCAACATGCTCAAGTTTCTTGTGATGTTTCTCCTCCTTCTTATAATCAACATCATCATCACGACTATTAGTATCATCACCATAACCGCCACCATAACCACCACCAGTGGTTGTTTTGGTGTCAGAGTCACCATAGCCACCACCATAACCGCCACCAGTGGTTGTTTTGGTGTCAGAGTCACCATAGCCACCACCATAGCCACCACCGGTAGTTTTTCTGGTGTCAGAGTCATCATAGCCACCACCACCATAGCCGCCACCAGTAGTTTTTCTGGTGTCAGAGTCATCATAGCCACCACCACCATAGCCGCCGCCAGTAGTTTTTCTGGTGTCAGAGTCATCATAGCCACCACTACCATAGCCGCCACCGGTAGTTTTTCTGGTGTCAGAGTCACCATATCCGCCACCGTAGCCGCCACCGGTGGTTGTTTTGGTGTCAGTGTATCCACTACCAGTACCACCATAGTTATCAGATGTTTCATAATTAGTTGTTGTTTTATTGTAACCAGTTCCATAGTCACCACCGGGATTTTCATTACCATATGATGTTTTGTTGTAACTAGATTCATTGTCACCACTAGAAAACTCATCATTGTGTGATGTCTTTCGGTAATTACCGGAGTCATAGTCATCGCCGGTAGGTCTCTTATCTTCATCCTTTTTGTGGTGGAAGAGGCCGCGGCTACGGTGCTCCTCTTCAGCCATGGTGTAGTTGCAAAGGGAGAATTGATAAAAGGATATAGAAAAATGAATTTAAGGAGAGATTGATATCCCCGAGTACTCTGCGTTGTATTACCTTACTTTTGTTTTCTTTTTT
TARGET=778,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1689
PRIMER_LEFT_PENALTY=0.050763
PRIMER_RIGHT_PENALTY=0.118120
PRIMER_LEFT_SEQUENCE=TGGTTGTTTTGGTGTCAGAGTC
PRIMER_RIGHT_SEQUENCE=TGGTAGTGGTGGCTATGATGAC
PRIMER_LEFT=697,22
PRIMER_RIGHT=896,22
PRIMER_LEFT_TM=60.051
PRIMER_RIGHT_TM=59.882
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=200
PRIMER_PAIR_PENALTY_1=0.1689
PRIMER_LEFT_1_PENALTY=0.050763
PRIMER_RIGHT_1_PENALTY=0.118120
PRIMER_LEFT_1_SEQUENCE=TGGTTGTTTTGGTGTCAGAGTC
PRIMER_RIGHT_1_SEQUENCE=TGGTAGTGGTGGCTATGATGAC
PRIMER_LEFT_1=646,22
PRIMER_RIGHT_1=896,22
PRIMER_LEFT_1_TM=60.051
PRIMER_RIGHT_1_TM=59.882
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=0.3614
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.141511
PRIMER_LEFT_2_SEQUENCE=CTCACCAACATGCTCAAGTTTC
PRIMER_RIGHT_2_SEQUENCE=TGGCTATGATGACTCTGACACC
PRIMER_LEFT_2=536,22
PRIMER_RIGHT_2=833,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=60.142
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=0.3614
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=0.141511
PRIMER_LEFT_3_SEQUENCE=CTCACCAACATGCTCAAGTTTC
PRIMER_RIGHT_3_SEQUENCE=GGCTATGATGACTCTGACACCA
PRIMER_LEFT_3=536,22
PRIMER_RIGHT_3=832,22
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=60.142
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.3614
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=0.141511
PRIMER_LEFT_4_SEQUENCE=CTCACCAACATGCTCAAGTTTC
PRIMER_RIGHT_4_SEQUENCE=GGTGGCTATGATGACTCTGACA
PRIMER_LEFT_4=536,22
PRIMER_RIGHT_4=835,22
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=60.142
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=300
=
PRIMER_SEQUENCE_ID=SSR_01628 Ps_contig_mira-and-tgicl-ass_franssen_15465
SEQUENCE=TACGATAAATACTTACCGGCAGAACTAGTAATTTTATTGAAATCATCTCACATTCTTTTACAATAGTATATTGTGTACTTAACAATTAACACAACATATAGATTCATACATACATGCATCATCTATATGATATCAATCTGGGAATTACATTCTCCAAACCACTCAAATTAAACTGCAATTAGATGAACTCAATACTAATAAATACACATAAACAACCCAAGAGCATAATTAATTATAGGGTAAAAGCTAAGCAGAATGAATATTTCCATAATGCTTGTATGCTTATTATTATATAGTTATATTATATACACATATCTTCAGCCAAAGAAATGGTGTTTTTTCTTTCCATGGGCTTCTTCATCTTCCTCCTTGGATTCTTTTTTCTCATGATGTTCATGAAAGGCAAATCCACCAGATCCCACTGCAGCAGCTGCTGCAACCTCTTCTTCTATCTTGTGCCTGTGAGCGTTCTCAGGATCCTTCCCTGATTTGTGCTTCTCATACAAAGCATAACCACCGGCAGCTACAGCACCAAACTCACCAACATGCTCAAGTTTCTTGTGATGTTTCTCCTCCTTCTTATAATCAACATCATCATCACGACTATTAGTATCATCACCATAACCGCCACCATAACCACCACCAGTGGTTGTTTTGGTGTCAGAGTCACCATAGCCACCACCATAACCGCCACCAGTGGTTGTTTTGGTGTCAGAGTCACCATAGCCACCACCATAGCCACCACCGGTAGTTTTTCTGGTGTCAGAGTCATCATAGCCACCACCACCATAGCCGCCACCAGTAGTTTTTCTGGTGTCAGAGTCATCATAGCCACCACCACCATAGCCGCCGCCAGTAGTTTTTCTGGTGTCAGAGTCATCATAGCCACCACTACCATAGCCGCCACCGGTAGTTTTTCTGGTGTCAGAGTCACCATATCCGCCACCGTAGCCGCCACCGGTGGTTGTTTTGGTGTCAGTGTATCCACTACCAGTACCACCATAGTTATCAGATGTTTCATAATTAGTTGTTGTTTTATTGTAACCAGTTCCATAGTCACCACCGGGATTTTCATTACCATATGATGTTTTGTTGTAACTAGATTCATTGTCACCACTAGAAAACTCATCATTGTGTGATGTCTTTCGGTAATTACCGGAGTCATAGTCATCGCCGGTAGGTCTCTTATCTTCATCCTTTTTGTGGTGGAAGAGGCCGCGGCTACGGTGCTCCTCTTCAGCCATGGTGTAGTTGCAAAGGGAGAATTGATAAAAGGATATAGAAAAATGAATTTAAGGAGAGATTGATATCCCCGAGTACTCTGCGTTGTATTACCTTACTTTTGTTTTCTTTTTT
TARGET=832,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1689
PRIMER_LEFT_PENALTY=0.050763
PRIMER_RIGHT_PENALTY=0.118120
PRIMER_LEFT_SEQUENCE=TGGTTGTTTTGGTGTCAGAGTC
PRIMER_RIGHT_SEQUENCE=TGGTAGTGGTGGCTATGATGAC
PRIMER_LEFT=697,22
PRIMER_RIGHT=896,22
PRIMER_LEFT_TM=60.051
PRIMER_RIGHT_TM=59.882
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=200
PRIMER_PAIR_PENALTY_1=0.1689
PRIMER_LEFT_1_PENALTY=0.050763
PRIMER_RIGHT_1_PENALTY=0.118120
PRIMER_LEFT_1_SEQUENCE=TGGTTGTTTTGGTGTCAGAGTC
PRIMER_RIGHT_1_SEQUENCE=TGGTAGTGGTGGCTATGATGAC
PRIMER_LEFT_1=646,22
PRIMER_RIGHT_1=896,22
PRIMER_LEFT_1_TM=60.051
PRIMER_RIGHT_1_TM=59.882
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=0.3089
PRIMER_LEFT_2_PENALTY=0.141511
PRIMER_RIGHT_2_PENALTY=0.167348
PRIMER_LEFT_2_SEQUENCE=TCAGAGTCATCATAGCCACCAC
PRIMER_RIGHT_2_SEQUENCE=ACACTGACACCAAAACAACCAC
PRIMER_LEFT_2=762,22
PRIMER_RIGHT_2=981,22
PRIMER_LEFT_2_TM=60.142
PRIMER_RIGHT_2_TM=59.833
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.3089
PRIMER_LEFT_3_PENALTY=0.141511
PRIMER_RIGHT_3_PENALTY=0.167348
PRIMER_LEFT_3_SEQUENCE=GTCAGAGTCATCATAGCCACCA
PRIMER_RIGHT_3_SEQUENCE=ACACTGACACCAAAACAACCAC
PRIMER_LEFT_3=761,22
PRIMER_RIGHT_3=981,22
PRIMER_LEFT_3_TM=60.142
PRIMER_RIGHT_3_TM=59.833
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.3089
PRIMER_LEFT_4_PENALTY=0.141511
PRIMER_RIGHT_4_PENALTY=0.167348
PRIMER_LEFT_4_SEQUENCE=TGTCAGAGTCATCATAGCCACC
PRIMER_RIGHT_4_SEQUENCE=ACACTGACACCAAAACAACCAC
PRIMER_LEFT_4=760,22
PRIMER_RIGHT_4=981,22
PRIMER_LEFT_4_TM=60.142
PRIMER_RIGHT_4_TM=59.833
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_01629 Ps_contig_mira-and-tgicl-ass_franssen_15486
SEQUENCE=GTMRRSKTAACAAATAAAGAATAGATAAGAGTAATGAGAATCAAGACGGACCCGTACGGATTTCTTACTGGTTAGGGATACAAAGTTGTGGAGAGCAAGAAGGACATGGTATTGGGTTCATTTCAAAGATACTTTAGCATGAGGCACCAAAACTCCAAGATTCAGGGTTTATACGATCACTGTAAAAACACTTTCTCTCCCTCCGGAATACCCTCTCCTTCTTCCCAACCTTTGCACAAGCTTTCTTTAATTCTTGACACGATTCGTCCCGCTGATGTTGGGCTTAAGAAGGAAGCTGCAGATGATGATCGAGGGCTTGGATTTTTCGGTGTTAACCAACAGAATAGAATAGCTCGATGGGCTAAGCCAATAACATACATCGACATTCATGAATCTGATAGTTTTACGATGTGTATATTTTGTTTTCCAACTTCTTCAGTTATTCCACTTCATGACCATCCGGGAATGACTGTATTTAGCAAACTTCTCTATGGATCTTTACATGTGAAAGCTTATGATTGGGTCGAGCCACCCTGCATTGTAGAAAGCAAGGGACCAGGACATCCTCAAGTAAGACTAGCAAAGTTAGCAGTTGATAAGGTTTTGAATGCACCGTGTGAGCCATCGGTTTTATATCCAAAGCATGGTGGAAATCTTCACTGTTTCACAGCAGTAAGACCTTGTGCGATGCTGGACATTCTCGCGCCTCCTTACAAAGAATATGAAGGAAGGAAGTGTACTTATTATCACGACTATCCTTATTCAACATTCTCTGCGGGAAATGGACCTGTATGTGTCGGTGAAGAGGATGAATATGCTTGGCTTGCAGAGACAGAACCTAGTGATCTTTATATGAATAGTGGTGTATATGCTGGTCCACCTATTAAGCCATAGTTTTGCTGCTTGGATTTTAAAGCAATTGCATGTTTCTTTGTTGTAAATTGTGGATAATATTAATGTTGGAAATTTCTAGAGTCTGTTTGATTACAAAAAAAAACCTGTCTTTTTACACATTTAAAAAATAAGGAAACAAAAATGAATTTTGTAGAATAAAAAAATTTAAAAAACAAACAAAATGAAACAAAGTAAAAA
TARGET=724,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01630 Ps_contig_mira-and-tgicl-ass_franssen_15503
SEQUENCE=GTCGGGGAAGCCCCTSRTCCTTATTCCCTACGCTCCTTGCCTCAGTTGCCGAGCAAGATATCACGCTGTCCGAGCTTGACTCTGAGAACCGGAAAATGAAAGTTGAGCTTGAGGAATTTAGAACAGAGGCAACTCACTTGAAGAATCAGCAGGCCACAATACGGAGACTTGAAGAGCGAAGCAGGCAATTAGAACAACAGATGGAAGAGAAAGTAAAGGAAATTGTGGAGATTAAGCATCGCAATCTAGCAGAGGAGAACCAAAAAACATTAGAAATTTTAAAAGAACGGGAACAACTACTACAGGACCAACTGCAGAGTGCTAAAGAAAGTGTTTCAAATATGAAGAAATTACATGAACTTGCACAAAACCAGTTGTTTGAACTCCGTGCTCAGTCTGAGGAAGAGAGGGCAGCTAAGCAAGCAGAGGCCAATCTGCTGATGGATGAAGTAGAACGAGCACAAACAATGCTTCTTGGTTTGGAGAGAGAGAAGGGAGTACTTCGATCTCAGTTAGTAACAGCAAATGAAGATTCTGAAAATAAAAAGAGTGATAATTTAGACTCAAATAATGCACTTGAGAACTCTTTAATTGCCAAAGAGAAGTTAATCTCTGAATTGAACATGGAGCTACACAATATTGAAACAACCTTATCAAATGAACGGGAAGAACACATTAATGATGTGAAGAAATTTACTGCAATGCTCAATGAAAAGGAAGCTTCTATTGTGGCAATGAAGAAAGAGCTTCACACAAGGCCAAACAAGAARAAATGTTGATGATGAAAATGCTAGACTTACATAGAAAAAATTAAAAAAAAACT
TARGET=483,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01631 Ps_contig_mira-and-tgicl-ass_franssen_15510
SEQUENCE=ATACGTAACTAGATATTTGGTGAGATTATTCTAAGTTTTTTTTTTTAATTTTTTATTAACTGAAAATTAATAAGCTATTATTATATGGTTCTGTTTATCTGAGATGCTTGAAACATAACTTTGTTTAGGTAAACAAATGCTGGAATTCCTGGAGTTCACATTGACAGTGAGGCTCTGGCTGCTGCTAACATTGTGGAGGGAAATCGTGGCCAGATTGATTGGAGTAAAATACTAACTGAGAGCTATGAACAAACCGAAGCAGGTATTAAAACAAACTACTACGGAGCAAAGGAATTAACCGAAGCACTTATTCCCCTTCTCCAGCTTTCAAGCTCACCAACAATTGTCAATGTTTCATCATCCATGGGAAGATTAGAGAATATTCCGAACGGATGGCCTAAGGAAGTGCTGAGTGATGTTGAGAATCTTACTGAAGAAAAAATTGATGAAGTATTGAACCAATTTCTAAAGGATTTTAAAGAGGGTTCAATAGTAGAAAACAAAGGTTGGCCTATTAATATGTCTACATATATCATCTCGAAAGCGGCTCTAAGTGCCTACACAAGAGTTCTGGCTAAAAAGTTTCCGTCAATACGCGTCAATGCTGTTTGTCCTGGCTATGTGAAAACAGATATAAACTACAATACAGGTCGTTTAACACCTGATGAAGGCGCCGAGTCGATTGTGAGGTTGGCATTGTTACCTGAGAATACTTCTGGCTTCTTTTTTGTTAGAAATGAAGAGAAACCATTTTGATCTTAGAGAACAAAATGTGATGGATATTTGAAGGGAAGTTATGAGGTGAAGAGAACTACTTTGTGTTGAAAATTTTCTACTTGTGTTGTACTAAAGAGAATATAAGTTCACTTTGTCACCTTAGCGTAATTAATCATTGTGCCTCCTCTACTATATAAACAGCTCGAAGCTTTAGTCTTAACATATCAGAAAATAAACTTTACATTATTGCATCTTAGTTTATTTATTTTTTCTTTTAATTTTAGTTTTGTTTATCGCATTAGTTTAAGCTTTATTTTTTGTTGTTGTTGTTATAATCCTTTTG
TARGET=1035,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01632 Ps_contig_mira-and-tgicl-ass_franssen_15516
SEQUENCE=GGAGGGACTCCAATTAAGCTCTATAAACTAAATATTCTTCAACTTATTTCTCCAAATTAAATTTCTTTACTCCCAAATTCTCAAACCTCATCCAAACAAAAATGGTTGATGTTCTAAGCCCTTTCACAATCCCATCTCCACCTCCACCATCTCCTTTATCAAACCAAAACAACAACACCATACCCATGTTATACTATAGTCTAGTAGTGATGGGAGTTGCAGCCATAGTTCTAGCAATTTACAACATCATCATCATCAAACGTTGCAATAGAAACAACCACAATAATCAACCACAACCCACAAGAGTAAATCTTAGTAGTACGAGGTTTGAAAACCAACAAAGAAACTTACTTTCAAGCTTCAAGTATAGGAAAGACATGGTTACAAAAGAAGAAGAAGAAAAGGGGTATGATGAATATGAATGTTCTGTTTGTTTATCGGTTTATGAAGAAGGAGAAGATGTTAGGAAGCTACCAAGGTGTAAACATAGTTTTCATGCTGTTTGTATAGATATGTGGCTTTATTCTCATTTTGATTGTCCAATTTGTAGAACATCTGTTGGTTCTTTTTGTGATGGGATGATTGAATCTGGTGGTAGCATCTCTGTTTGATTTGATTTAGTTTAGAAATGTGTGCGTGGTATGTTGGGTTGTCTCTAGGACAGGACATTGAGTGATTAACACCCCATCACACTAGTTTGGTTCATTTTTAGTTCTTTATTTGGAACAAGTTTTAGGTGGGATTGGATAAGAAAATATTGTTAC
TARGET=245,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0715
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.070748
PRIMER_LEFT_SEQUENCE=TGGTTGATGTTCTAAGCCCTTT
PRIMER_RIGHT_SEQUENCE=TTACTCTTGTGGGTTGTGGTTG
PRIMER_LEFT=102,22
PRIMER_RIGHT=308,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.929
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.2441
PRIMER_LEFT_1_PENALTY=0.173354
PRIMER_RIGHT_1_PENALTY=0.070748
PRIMER_LEFT_1_SEQUENCE=CACCTCCACCATCTCCTTTATC
PRIMER_RIGHT_1_SEQUENCE=TTACTCTTGTGGGTTGTGGTTG
PRIMER_LEFT_1=138,22
PRIMER_RIGHT_1=308,22
PRIMER_LEFT_1_TM=59.827
PRIMER_RIGHT_1_TM=59.929
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=171
PRIMER_PAIR_PENALTY_2=0.2441
PRIMER_LEFT_2_PENALTY=0.173354
PRIMER_RIGHT_2_PENALTY=0.070748
PRIMER_LEFT_2_SEQUENCE=ACCTCCACCATCTCCTTTATCA
PRIMER_RIGHT_2_SEQUENCE=TTACTCTTGTGGGTTGTGGTTG
PRIMER_LEFT_2=139,22
PRIMER_RIGHT_2=308,22
PRIMER_LEFT_2_TM=59.827
PRIMER_RIGHT_2_TM=59.929
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.2631
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.262407
PRIMER_LEFT_3_SEQUENCE=TGGTTGATGTTCTAAGCCCTTT
PRIMER_RIGHT_3_SEQUENCE=TCTTGTGGGTTGTGGTTGATTA
PRIMER_LEFT_3=102,22
PRIMER_RIGHT_3=304,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.262
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.3242
PRIMER_LEFT_4_PENALTY=0.253482
PRIMER_RIGHT_4_PENALTY=0.070748
PRIMER_LEFT_4_SEQUENCE=TGATGTTCTAAGCCCTTTCACA
PRIMER_RIGHT_4_SEQUENCE=TTACTCTTGTGGGTTGTGGTTG
PRIMER_LEFT_4=106,22
PRIMER_RIGHT_4=308,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=59.929
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_01633 Ps_contig_mira-and-tgicl-ass_franssen_15516
SEQUENCE=GGAGGGACTCCAATTAAGCTCTATAAACTAAATATTCTTCAACTTATTTCTCCAAATTAAATTTCTTTACTCCCAAATTCTCAAACCTCATCCAAACAAAAATGGTTGATGTTCTAAGCCCTTTCACAATCCCATCTCCACCTCCACCATCTCCTTTATCAAACCAAAACAACAACACCATACCCATGTTATACTATAGTCTAGTAGTGATGGGAGTTGCAGCCATAGTTCTAGCAATTTACAACATCATCATCATCAAACGTTGCAATAGAAACAACCACAATAATCAACCACAACCCACAAGAGTAAATCTTAGTAGTACGAGGTTTGAAAACCAACAAAGAAACTTACTTTCAAGCTTCAAGTATAGGAAAGACATGGTTACAAAAGAAGAAGAAGAAAAGGGGTATGATGAATATGAATGTTCTGTTTGTTTATCGGTTTATGAAGAAGGAGAAGATGTTAGGAAGCTACCAAGGTGTAAACATAGTTTTCATGCTGTTTGTATAGATATGTGGCTTTATTCTCATTTTGATTGTCCAATTTGTAGAACATCTGTTGGTTCTTTTTGTGATGGGATGATTGAATCTGGTGGTAGCATCTCTGTTTGATTTGATTTAGTTTAGAAATGTGTGCGTGGTATGTTGGGTTGTCTCTAGGACAGGACATTGAGTGATTAACACCCCATCACACTAGTTTGGTTCATTTTTAGTTCTTTATTTGGAACAAGTTTTAGGTGGGATTGGATAAGAAAATATTGTTAC
TARGET=388,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5590
PRIMER_LEFT_PENALTY=0.070748
PRIMER_RIGHT_PENALTY=0.488216
PRIMER_LEFT_SEQUENCE=CAACCACAACCCACAAGAGTAA
PRIMER_RIGHT_SEQUENCE=CCATCACAAAAAGAACCAACAG
PRIMER_LEFT=287,22
PRIMER_RIGHT=576,22
PRIMER_LEFT_TM=59.929
PRIMER_RIGHT_TM=59.512
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=290
PRIMER_PAIR_PENALTY_1=0.7506
PRIMER_LEFT_1_PENALTY=0.262407
PRIMER_RIGHT_1_PENALTY=0.488216
PRIMER_LEFT_1_SEQUENCE=TAATCAACCACAACCCACAAGA
PRIMER_RIGHT_1_SEQUENCE=CCATCACAAAAAGAACCAACAG
PRIMER_LEFT_1=283,22
PRIMER_RIGHT_1=576,22
PRIMER_LEFT_1_TM=60.262
PRIMER_RIGHT_1_TM=59.512
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=0.9323
PRIMER_LEFT_2_PENALTY=0.444122
PRIMER_RIGHT_2_PENALTY=0.488216
PRIMER_LEFT_2_SEQUENCE=TCAACCACAACCCACAAGAGTA
PRIMER_RIGHT_2_SEQUENCE=CCATCACAAAAAGAACCAACAG
PRIMER_LEFT_2=286,22
PRIMER_RIGHT_2=576,22
PRIMER_LEFT_2_TM=60.444
PRIMER_RIGHT_2_TM=59.512
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=1.0019
PRIMER_LEFT_3_PENALTY=0.513718
PRIMER_RIGHT_3_PENALTY=0.488216
PRIMER_LEFT_3_SEQUENCE=CAATAATCAACCACAACCCACA
PRIMER_RIGHT_3_SEQUENCE=CCATCACAAAAAGAACCAACAG
PRIMER_LEFT_3=280,22
PRIMER_RIGHT_3=576,22
PRIMER_LEFT_3_TM=60.514
PRIMER_RIGHT_3_TM=59.512
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=1.0019
PRIMER_LEFT_4_PENALTY=0.513718
PRIMER_RIGHT_4_PENALTY=0.488216
PRIMER_LEFT_4_SEQUENCE=CACAATAATCAACCACAACCCA
PRIMER_RIGHT_4_SEQUENCE=CCATCACAAAAAGAACCAACAG
PRIMER_LEFT_4=278,22
PRIMER_RIGHT_4=576,22
PRIMER_LEFT_4_TM=60.514
PRIMER_RIGHT_4_TM=59.512
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_01634 Ps_contig_mira-and-tgicl-ass_franssen_15522
SEQUENCE=CGGGGTAAGTTTTGTATAAGTTTACTTGTCTGAGATTTACYGKRACTCTCACAGGCGTAACCTTATCCAACCTATTCATGAGAACTTGAGCGAGCCAAGCATTTCGACCGCCGCCAACTAAAATTATTTCATATCCCTCTCTTTTAATTTTCATTCCTTCCATCATCATCATCATGTTGAAGTTCAGTATATGTCCCATCAAAACCCTAACCCATCTCAACTGCTTACCTTCCCCACAACAATCACCATCCTCTCTACATTTCGAATCAACCCCATTCCAAGTCTCCTACCTCATCAACAATTTCGATTTCTCCCCACAATCCGCTTCCAAACTTTGCTCCACCTACCGTCTTAATTTCAAAACCACCCAAAATCCTGACTCTGTTATCAACTTCTTCACAGACTACGGTTTCTCTATTTCCCAGTTACGCCACATCATTGCCAAGGAACCGTGGCTGCTTTCATGCAACTTCTCCAAAAGGGTCTTGCCAAAGTTTCTATTTTTTCTCTCCAAAGGT
TARGET=161,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01635 Ps_contig_mira-and-tgicl-ass_franssen_15526
SEQUENCE=CCTTAAGATCATTCGCAGGCATTAAACATTTCCTTAAGATCATTCGCAGGCATTAAACATTTGTCTAGAAGTCTTACTCTTCTACATTGTATTGGCTGTTTCTTTTGCAGGCATGAGAATGAGATTCTTGAGGCTGCTAATCAGCCCCTTCCTGATGATGATGACGATGCTTTTGAATAGGAAGGACCTTCGAGAGTGCTGGCCTATGTGTGTTGATTTGTGCCGTTATATGCTGTAATGTTATGTGTTTTGTGGTTGAACCGTTGACTATTTTTTTGATATAGATTTATGGATTTCAATCATCATGTGATTGTAGTTTGTTTTTACTTTTATCCCAAACCCTCAACTTAATGGCTGCTATGTATTCCTTTTACTCTGCCGTGCCTAAGTTAAGGTAAAAAAAGGGTTTAA
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0420
PRIMER_LEFT_PENALTY=0.010294
PRIMER_RIGHT_PENALTY=0.031678
PRIMER_LEFT_SEQUENCE=GAATGAGATTCTTGAGGCTGCT
PRIMER_RIGHT_SEQUENCE=CTTAACTTAGGCACGGCAGAGT
PRIMER_LEFT=116,22
PRIMER_RIGHT=393,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=278
PRIMER_PAIR_PENALTY_1=0.0420
PRIMER_LEFT_1_PENALTY=0.010294
PRIMER_RIGHT_1_PENALTY=0.031678
PRIMER_LEFT_1_SEQUENCE=AGAATGAGATTCTTGAGGCTGC
PRIMER_RIGHT_1_SEQUENCE=CTTAACTTAGGCACGGCAGAGT
PRIMER_LEFT_1=115,22
PRIMER_RIGHT_1=393,22
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.1595
PRIMER_LEFT_2_PENALTY=0.010294
PRIMER_RIGHT_2_PENALTY=0.149184
PRIMER_LEFT_2_SEQUENCE=GAATGAGATTCTTGAGGCTGCT
PRIMER_RIGHT_2_SEQUENCE=TAGCAGCCATTAAGTTGAGGGT
PRIMER_LEFT_2=116,22
PRIMER_RIGHT_2=359,22
PRIMER_LEFT_2_TM=59.990
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.1595
PRIMER_LEFT_3_PENALTY=0.010294
PRIMER_RIGHT_3_PENALTY=0.149184
PRIMER_LEFT_3_SEQUENCE=AGAATGAGATTCTTGAGGCTGC
PRIMER_RIGHT_3_SEQUENCE=TAGCAGCCATTAAGTTGAGGGT
PRIMER_LEFT_3=115,22
PRIMER_RIGHT_3=359,22
PRIMER_LEFT_3_TM=59.990
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.2136
PRIMER_LEFT_4_PENALTY=0.010294
PRIMER_RIGHT_4_PENALTY=0.203283
PRIMER_LEFT_4_SEQUENCE=GAATGAGATTCTTGAGGCTGCT
PRIMER_RIGHT_4_SEQUENCE=ATAACGGCACAAATCAACACAC
PRIMER_LEFT_4=116,22
PRIMER_RIGHT_4=228,22
PRIMER_LEFT_4_TM=59.990
PRIMER_RIGHT_4_TM=59.797
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=113
=
PRIMER_SEQUENCE_ID=SSR_01636 Ps_contig_mira-and-tgicl-ass_franssen_15532
SEQUENCE=ACATTTTCATTTGCGGAAGATATAGGAGATAAAGACGAGTCCTTCCATGAGAATTTCACGATACATGTATTATCCCAAAATGAGGATGAGGTCTCACAGAACCAAGAAATTCAAGAAATCCAAGTTATTGACAAGAAGATACAACAATGGTCTAAAGGGAAGGAAGGAAACATACGCTCGCTGCTGTCAACATTACAATACGTTCTTTGGCCAAACTGTGGCTGGAAGCCTGTGCCTCTCGTCGATATAATTGAAGGGAATGCGGTGAAAAGGTCTTATCAGAGAGCTTTACTCAGTCTGCATCCGGATAAGTTGCAGCAAAAGGGTGCGACTTCAGACCAAAAATACATCGCAGAAAAAGTTTTTGATATTTTACAGGAGGCATGGACTCAATTCAACATGGTTGGTCAACTCTAATATGCCTGTCAATACTCAATACCAACATGAGAAACTATCTTCTACAGTCTGTCTACAAGGGTATATGTGTTGTACTTATTGGAACTGTAAGTGTTCTTCATATTTCTGTAATTGAATTAGTAAAAAAAATAATAAATGAATTRCTCCTCTGGTATGTATACAAATTATAAAATATTAGTCTATTTTTTWWAMG
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01637 Ps_contig_mira-and-tgicl-ass_franssen_15534
SEQUENCE=GACACGAGTTAAAAATGGATGTGATAATGTTCGGGATTATAATCGCCGTTATCATACTGTTCTTAATTATCGTCTGCTACATCACAATTGAAGGCACTTGCAACTCACGCTCTTAATTCAACTGAAGTCCTACTTTTTCTTTTCACCCTTTCAATTTCATGGCAATGGCATTGAGTAGTAGTAGTAGAAGAAGAAAGAGAGTTGAATCGTCGTTACTCGTTTTCTTGTTGTTTGTTTCTATGCTCCTTTTTGGATCTGGTAACTGCACATCAAGGTCACTCTCATTCTGAATCTAATATTCATCATCATCACTGTGGCGGTGGAGATGGTCATCACCATCATCATCACCATCACCATGATCATGATGATCATGACCATGATCATGATCATGGCCATGTTCGCCAGTCGATGCTTCCGGAGGAGTTAGCGGAAGAGGAGGATATGAAACTCTACGGGTTTGGTTTACCACATCACGACCTTCACTTTACTGGCTCCACTGAATTATCGGGTTTAGGTCTTTGGTTGAATGCATTGGGCTGTTCATTTCTGGTTAGCATGGCTTCCCTGATTTGCCTCATTTTGCTGCCTGTAATTTTTATTCAAGGGAAACCATCAAAGGCGGTTGTCGATTCATTGGCTTTATTTGGGGCAGGAGCTATGTTGGGGGATGCTTTTCTTCACCAACTACCACACGCTTTTGGTGGTGAACATTCCCATTCTCACAGCGACCATGCAGACCATGACCATGGCCATGATGCTAGTTCTGGACATGGACATTCACATTCCTTAGCTGATCTTTCTACAGGAATGTCAATCCTAGCTGGAATTGTACTCTTTCTTCTTGTGGAGAAGCTAGTGAGGTATGTCGAAGAAAACTCAGGAGGAGCTAATTCGTGGGGGCATGGACACCACCACCATAACCACAACAGCAAAGAAAAAAATTGGAAGATGATAATAATTCTGATGTCAACAATCAGTCACAGTCTATCAAAACAAAGAAAGAGAGATTGGTAGATGAGGGGAAAGAAGACAATCAGGTATCACATGACTCATTGAAGGGAGATAAGCTAGCTCAAACTGAATCTTCTCTTAGAAAGAGAATTGGAAGCAATACAACCAAAGATGATAGTCTCAATGATAATGCTGAAGATAGCTCCACTGATAATATCAAACCTTCAATTGTTAAAGAACCAGTTAGACCAGCAAGTAGTCTTGTGTTTGGCTATCTCAATCTTTTCTCTGATGGAGTTCATAATTTCACTGATGGAATTGCATTAGGAAGTGCATTCATGCTTCATGGATCAGTTGGTGGCTGGTCTAGAACTCTGTTTTTGTTGGCACATGAAATTCCTCAAGAGGTTGGTGATTTTGGTATTTTGATAAGATCTGGTTTCAGCATACCAAAAGCTTTGTTCTTCAACTTTTTATCACGCACTTGTGGCACTCGCAGGCACTGCATTGGCTCTGGTTTGGGGGAAAGATCCTGGGCAGTCATCTCTGATTGAGGGATTTACAGCAGGTGGATTTATATACATAGCCATTGCGAGGAGTG
TARGET=174,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0844
PRIMER_LEFT_PENALTY=0.084327
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=GGATGTGATAATGTTCGGGATT
PRIMER_RIGHT_SEQUENCE=GATGTGCAGTTACCAGATCCAA
PRIMER_LEFT=16,22
PRIMER_RIGHT=270,22
PRIMER_LEFT_TM=59.916
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=255
PRIMER_PAIR_PENALTY_1=0.0844
PRIMER_LEFT_1_PENALTY=0.084327
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=GGATGTGATAATGTTCGGGATT
PRIMER_RIGHT_1_SEQUENCE=TTGATGTGCAGTTACCAGATCC
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=59.916
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.0851
PRIMER_LEFT_2_PENALTY=0.084327
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=GGATGTGATAATGTTCGGGATT
PRIMER_RIGHT_2_SEQUENCE=GCAGTTACCAGATCCAAAAAGG
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=265,22
PRIMER_LEFT_2_TM=59.916
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.1184
PRIMER_LEFT_3_PENALTY=0.084327
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=GGATGTGATAATGTTCGGGATT
PRIMER_RIGHT_3_SEQUENCE=ACCAGATCCAAAAAGGAGCATA
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=59.916
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.1184
PRIMER_LEFT_4_PENALTY=0.084327
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=GGATGTGATAATGTTCGGGATT
PRIMER_RIGHT_4_SEQUENCE=TACCAGATCCAAAAAGGAGCAT
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=260,22
PRIMER_LEFT_4_TM=59.916
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_01638 Ps_contig_mira-and-tgicl-ass_franssen_15534
SEQUENCE=GACACGAGTTAAAAATGGATGTGATAATGTTCGGGATTATAATCGCCGTTATCATACTGTTCTTAATTATCGTCTGCTACATCACAATTGAAGGCACTTGCAACTCACGCTCTTAATTCAACTGAAGTCCTACTTTTTCTTTTCACCCTTTCAATTTCATGGCAATGGCATTGAGTAGTAGTAGTAGAAGAAGAAAGAGAGTTGAATCGTCGTTACTCGTTTTCTTGTTGTTTGTTTCTATGCTCCTTTTTGGATCTGGTAACTGCACATCAAGGTCACTCTCATTCTGAATCTAATATTCATCATCATCACTGTGGCGGTGGAGATGGTCATCACCATCATCATCACCATCACCATGATCATGATGATCATGACCATGATCATGATCATGGCCATGTTCGCCAGTCGATGCTTCCGGAGGAGTTAGCGGAAGAGGAGGATATGAAACTCTACGGGTTTGGTTTACCACATCACGACCTTCACTTTACTGGCTCCACTGAATTATCGGGTTTAGGTCTTTGGTTGAATGCATTGGGCTGTTCATTTCTGGTTAGCATGGCTTCCCTGATTTGCCTCATTTTGCTGCCTGTAATTTTTATTCAAGGGAAACCATCAAAGGCGGTTGTCGATTCATTGGCTTTATTTGGGGCAGGAGCTATGTTGGGGGATGCTTTTCTTCACCAACTACCACACGCTTTTGGTGGTGAACATTCCCATTCTCACAGCGACCATGCAGACCATGACCATGGCCATGATGCTAGTTCTGGACATGGACATTCACATTCCTTAGCTGATCTTTCTACAGGAATGTCAATCCTAGCTGGAATTGTACTCTTTCTTCTTGTGGAGAAGCTAGTGAGGTATGTCGAAGAAAACTCAGGAGGAGCTAATTCGTGGGGGCATGGACACCACCACCATAACCACAACAGCAAAGAAAAAAATTGGAAGATGATAATAATTCTGATGTCAACAATCAGTCACAGTCTATCAAAACAAAGAAAGAGAGATTGGTAGATGAGGGGAAAGAAGACAATCAGGTATCACATGACTCATTGAAGGGAGATAAGCTAGCTCAAACTGAATCTTCTCTTAGAAAGAGAATTGGAAGCAATACAACCAAAGATGATAGTCTCAATGATAATGCTGAAGATAGCTCCACTGATAATATCAAACCTTCAATTGTTAAAGAACCAGTTAGACCAGCAAGTAGTCTTGTGTTTGGCTATCTCAATCTTTTCTCTGATGGAGTTCATAATTTCACTGATGGAATTGCATTAGGAAGTGCATTCATGCTTCATGGATCAGTTGGTGGCTGGTCTAGAACTCTGTTTTTGTTGGCACATGAAATTCCTCAAGAGGTTGGTGATTTTGGTATTTTGATAAGATCTGGTTTCAGCATACCAAAAGCTTTGTTCTTCAACTTTTTATCACGCACTTGTGGCACTCGCAGGCACTGCATTGGCTCTGGTTTGGGGGAAAGATCCTGGGCAGTCATCTCTGATTGAGGGATTTACAGCAGGTGGATTTATATACATAGCCATTGCGAGGAGTG
TARGET=300,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0310
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=CCTTTTTGGATCTGGTAACTGC
PRIMER_RIGHT_SEQUENCE=TAAAGTGAAGGTCGTGATGTGG
PRIMER_LEFT=244,22
PRIMER_RIGHT=486,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.0351
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=GGATCTGGTAACTGCACATCAA
PRIMER_RIGHT_1_SEQUENCE=ATGTGGTAAACCAAACCCGTAG
PRIMER_LEFT_1=251,22
PRIMER_RIGHT_1=470,22
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=220
PRIMER_PAIR_PENALTY_2=0.0351
PRIMER_LEFT_2_PENALTY=0.000037
PRIMER_RIGHT_2_PENALTY=0.035021
PRIMER_LEFT_2_SEQUENCE=TTGGATCTGGTAACTGCACATC
PRIMER_RIGHT_2_SEQUENCE=ATGTGGTAAACCAAACCCGTAG
PRIMER_LEFT_2=249,22
PRIMER_RIGHT_2=470,22
PRIMER_LEFT_2_TM=60.000
PRIMER_RIGHT_2_TM=60.035
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.0358
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.035021
PRIMER_LEFT_3_SEQUENCE=CCTTTTTGGATCTGGTAACTGC
PRIMER_RIGHT_3_SEQUENCE=ATGTGGTAAACCAAACCCGTAG
PRIMER_LEFT_3=244,22
PRIMER_RIGHT_3=470,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.035
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.0644
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=TATGCTCCTTTTTGGATCTGGT
PRIMER_RIGHT_4_SEQUENCE=TAAAGTGAAGGTCGTGATGTGG
PRIMER_LEFT_4=238,22
PRIMER_RIGHT_4=486,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_01639 Ps_contig_mira-and-tgicl-ass_franssen_15537
SEQUENCE=CTTAATTTCAACACCTAAACCAAACTTCAAATTTACTAAAACCACAAACCAATTGCCATTAATTCTAATTACAACATAATAATACATAACCTACATTCAAACCAATACAATGTCAATTTCAAAATTACAAAAAATACCAACCATTAACAATCCTAAGTTCCAAGTCATTTTTCAACATCACCATACAATACGTCAATAACATGTCCTAGCATTCACATCCCACAATAGCTCCATGATTAACATGTCCAAGCATCAAATTTCCACAAGCAAGCCAATGATCACAACCCTGGTTGCATCAATGCTTCAATCCGGTGAGTTTTCTTGATTTCAATTCCATGCTCTTCACTTGACGCGTCATAATCTGAAGAAGAAGAAGAAGTTGAGGATGAACACAATCCAGACGTGAATCCGGAAAGTTGAAGTTGTGAATCGCGTTGTCGTTGCAGCTTTTTTACATAAGCGTTTTTTCGTTTTCTCCGTTTATGTTTGTTGAGCATCAAAAGCCTTCGATTATTGCTGATAGAAAAAATTGATTAAGGTAGGATAGGGTTACACAACATGCAGAGGGAAAAAAATTCACCCTTTGCAGGCTCATGTTACCTATAGCCTGGTTGATGTGGAGGTGTGAGCTTTTGGGGCTCATGTATCTCCCKCCKWCTTCTTCCCGTAGGAGACGGGTGTCA
TARGET=364,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01640 Ps_contig_mira-and-tgicl-ass_franssen_15548
SEQUENCE=GCTGCACCTTTTGGAGTCTCCTTTGTTGAATGAACAACCTGCCATCTACTAGGTTTATTAAGGACATCCTTCCAGGATTTCCTTTTGTCATGTTCCTCAGTTTGCTCGGAATGGATATCCTTTTCCGATGACTTGATTTCTTCATCCTCTCTTTCAGAAGAAGAATCACATTTTTGCTTAGAAGTTAAAGAAGCCTTCTTCACAAGAGGATAATCTTGAACAAAGTTAAAGGACTTTGTGCCATAACCTGAAACTGGTATTGAGGTTTGGAATGTTTGGGATCAGTACTGATTTGTCCAACAACATGAACAACGTCGTTTTGTTTGAGATGAAAGTTAGCAGTATGAGCTAAATCGCCTTGAAATATAACGGGGATCAAAAGGGAAGAAGGATTATGGTCAAGACTCGAGATAATGGTGGCGGCGAAGGGGTTTCCGTCGGGAGAAGTTTCGAACTGAACTGGCGTGTTAACGGAGCCGATTAGGTTGACAGAGTTGGCGAGTTTCGGTTGAAATGGAATCTCGGTGGGTTTTGAAAACTTTGATTTGGATTTGCGAGTGGTGGTGGTGGTGGAGGAATAATAGCTACTGTTTCGATATCGAAGACGGAGTAACGCCGAGTTCGATGAACACCACACTCGCCGGAGTGAATTCATTCCCCCCCGAC
TARGET=558,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5233
PRIMER_LEFT_PENALTY=0.035742
PRIMER_RIGHT_PENALTY=2.487598
PRIMER_LEFT_SEQUENCE=CCTTGAAATATAACGGGGATCA
PRIMER_RIGHT_SEQUENCE=GGCGTTACTCCGTCTTCGATA
PRIMER_LEFT=356,22
PRIMER_RIGHT=616,21
PRIMER_LEFT_TM=60.036
PRIMER_RIGHT_TM=61.488
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=2.5593
PRIMER_LEFT_1_PENALTY=0.071684
PRIMER_RIGHT_1_PENALTY=2.487598
PRIMER_LEFT_1_SEQUENCE=AGAAGTTTCGAACTGAACTGGC
PRIMER_RIGHT_1_SEQUENCE=GGCGTTACTCCGTCTTCGATA
PRIMER_LEFT_1=442,22
PRIMER_RIGHT_1=616,21
PRIMER_LEFT_1_TM=59.928
PRIMER_RIGHT_1_TM=61.488
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=175
PRIMER_PAIR_PENALTY_2=2.5772
PRIMER_LEFT_2_PENALTY=0.089612
PRIMER_RIGHT_2_PENALTY=2.487598
PRIMER_LEFT_2_SEQUENCE=GCAGTATGAGCTAAATCGCCTT
PRIMER_RIGHT_2_SEQUENCE=GGCGTTACTCCGTCTTCGATA
PRIMER_LEFT_2=338,22
PRIMER_RIGHT_2=616,21
PRIMER_LEFT_2_TM=59.910
PRIMER_RIGHT_2_TM=61.488
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=2.6675
PRIMER_LEFT_3_PENALTY=0.179943
PRIMER_RIGHT_3_PENALTY=2.487598
PRIMER_LEFT_3_SEQUENCE=GTTTCGGTTGAAATGGAATCTC
PRIMER_RIGHT_3_SEQUENCE=GGCGTTACTCCGTCTTCGATA
PRIMER_LEFT_3=501,22
PRIMER_RIGHT_3=616,21
PRIMER_LEFT_3_TM=59.820
PRIMER_RIGHT_3_TM=61.488
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=116
PRIMER_PAIR_PENALTY_4=2.7468
PRIMER_LEFT_4_PENALTY=0.259220
PRIMER_RIGHT_4_PENALTY=2.487598
PRIMER_LEFT_4_SEQUENCE=AATATAACGGGGATCAAAAGGG
PRIMER_RIGHT_4_SEQUENCE=GGCGTTACTCCGTCTTCGATA
PRIMER_LEFT_4=362,22
PRIMER_RIGHT_4=616,21
PRIMER_LEFT_4_TM=60.259
PRIMER_RIGHT_4_TM=61.488
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_01641 Ps_contig_mira-and-tgicl-ass_franssen_15564
SEQUENCE=CCGTAAAACCTAAAACCTAAAAAAAMAWKRMAACTHCAAAAATAGTTAAGCAAAAAGACCCTAGAATAATTCCATTTTTAATCAATCATTGATTCATAAATGGATACCACTAAGCATTATCATTATAATTCAAAGTTAAACCGAAGAAAATGATGCATTTGTACTTTAAATTTATATGAAATTAGGGTTTAAACTATATGAAATCCATTTGGTTATAAAAAGCTATATACCAAGCTTAGAAAAACTTTATTAATTATAGATCTGGGTGAAATTATAGATCTGAGTGAAACTACTCAAAATGCATACCATGAAACCCTAGAGAGAGAAATTGTAGAGAGAGAAAGTGAAAAGGAGAAGGGGTGGAATGTTGAAGATTCATTGAAAGGGTTGCTAGATCTGAGAAATTCATGCTAGAAACAGACAGATCCGAAGGAAAAAACCATAATTGTGAGAAGATCTAGCTAGCTAATGATGAAGAAGAAAAGAAAAAAGTGTTACATCTGACAAGAAAAACACCATCATAACACAATCAGAGAGTAAGAAAAAGGAGGTAATGATGAAAACCTTTTGAGGAAGAGAAAGAGAAATAAAATTGGTCAAAATGCTTCATCATTACACCAATCTATATAAGAACAGATGAATGGAAGTTTATAATTGAGGGGAATGAAGCCTGGTCCGAGAACACTTTACGATTCTTGACCATAGATAATTTATAGATCAAAAAGAAAAAGTCCTCGAAGCAGACAACATTCCCCGCAAAAAATAAAGCTTCTTTCTAAATAGTTTTTTTTCTTGAATTTCATGAGAGAGATAGATATATGAAGTTTTTTCACATGGTTTGTGTTCTCTTTTAACTCTTGAATCATCCCCCAAAAGTCTCGGCTACCTTCAAGGGGGAGTGAAAGAGAGAGAGAAGGGAATGAGTGGTGGTGGTGAMYCCGA
TARGET=904,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01642 Ps_contig_mira-and-tgicl-ass_franssen_15564
SEQUENCE=CCGTAAAACCTAAAACCTAAAAAAAMAWKRMAACTHCAAAAATAGTTAAGCAAAAAGACCCTAGAATAATTCCATTTTTAATCAATCATTGATTCATAAATGGATACCACTAAGCATTATCATTATAATTCAAAGTTAAACCGAAGAAAATGATGCATTTGTACTTTAAATTTATATGAAATTAGGGTTTAAACTATATGAAATCCATTTGGTTATAAAAAGCTATATACCAAGCTTAGAAAAACTTTATTAATTATAGATCTGGGTGAAATTATAGATCTGAGTGAAACTACTCAAAATGCATACCATGAAACCCTAGAGAGAGAAATTGTAGAGAGAGAAAGTGAAAAGGAGAAGGGGTGGAATGTTGAAGATTCATTGAAAGGGTTGCTAGATCTGAGAAATTCATGCTAGAAACAGACAGATCCGAAGGAAAAAACCATAATTGTGAGAAGATCTAGCTAGCTAATGATGAAGAAGAAAAGAAAAAAGTGTTACATCTGACAAGAAAAACACCATCATAACACAATCAGAGAGTAAGAAAAAGGAGGTAATGATGAAAACCTTTTGAGGAAGAGAAAGAGAAATAAAATTGGTCAAAATGCTTCATCATTACACCAATCTATATAAGAACAGATGAATGGAAGTTTATAATTGAGGGGAATGAAGCCTGGTCCGAGAACACTTTACGATTCTTGACCATAGATAATTTATAGATCAAAAAGAAAAAGTCCTCGAAGCAGACAACATTCCCCGCAAAAAATAAAGCTTCTTTCTAAATAGTTTTTTTTCTTGAATTTCATGAGAGAGATAGATATATGAAGTTTTTTCACATGGTTTGTGTTCTCTTTTAACTCTTGAATCATCCCCCAAAAGTCTCGGCTACCTTCAAGGGGGAGTGAAAGAGAGAGAGAAGGGAATGAGTGGTGGTGGTGAMYCCGA
TARGET=924,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01643 Ps_contig_mira-and-tgicl-ass_franssen_15574
SEQUENCE=GAAATTAGTAGGNGAAAAGAAGAGCAGGTNAAAACTTAATTACAATAAAGCACCAAATTTGAGAAGCAGGCAAATAAAGAGAAGATTCTTTGGTTGTCTNAAGTTCATGAGATTGCTGCCTTTGTGGTGACTCAATCCGGACATTACGAGGCAATTACAAAGAACAAATCTAACTACTACTACTTATCTGCCGAATCCGTGGCACTATTTACAGACCATCTTCCAAGCAGACCTAACTACATCGTCGGACAGATTGTGCATATCGAACACCAAATTGTGAAGGCATTGCCGGAGCACGGTAGGGTGACAACTTCTGACAAAGGGACTACTGACTGGTTGACCTTGAATTCAGGTTCAACTCCAAATCCATATGGTCTACCTGTTGGATGTGAATATTTTGTAGTGACGGTAGCCATGTTACCCGATACCGCCATTCGTTCATCCTCTCCTACTTCCTGATCCTGCTTCTTCATCATGGTAGTAGCAGATTAGACATGATGGAAGAATTCAATTGTATATAAACAATATTCAAAGTTTAAATGCTCGTTATGAGTTTTGAATCAGTTAACTATCTGTACAGAGCTCTCTTTA
TARGET=173,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3942
PRIMER_LEFT_PENALTY=0.269585
PRIMER_RIGHT_PENALTY=0.124655
PRIMER_LEFT_SEQUENCE=AGTTCATGAGATTGCTGCCTTT
PRIMER_RIGHT_SEQUENCE=TCTGCTTGGAAGATGGTCTGTA
PRIMER_LEFT=101,22
PRIMER_RIGHT=230,22
PRIMER_LEFT_TM=60.270
PRIMER_RIGHT_TM=59.875
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=0.3942
PRIMER_LEFT_1_PENALTY=0.269585
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=AAGTTCATGAGATTGCTGCCTT
PRIMER_RIGHT_1_SEQUENCE=TCTGCTTGGAAGATGGTCTGTA
PRIMER_LEFT_1=100,22
PRIMER_RIGHT_1=230,22
PRIMER_LEFT_1_TM=60.270
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.3999
PRIMER_LEFT_2_PENALTY=0.269585
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=AGTTCATGAGATTGCTGCCTTT
PRIMER_RIGHT_2_SEQUENCE=TTAGGTCTGCTTGGAAGATGGT
PRIMER_LEFT_2=101,22
PRIMER_RIGHT_2=235,22
PRIMER_LEFT_2_TM=60.270
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=135
PRIMER_PAIR_PENALTY_3=0.3999
PRIMER_LEFT_3_PENALTY=0.269585
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=AGTTCATGAGATTGCTGCCTTT
PRIMER_RIGHT_3_SEQUENCE=GTTAGGTCTGCTTGGAAGATGG
PRIMER_LEFT_3=101,22
PRIMER_RIGHT_3=236,22
PRIMER_LEFT_3_TM=60.270
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=0.3999
PRIMER_LEFT_4_PENALTY=0.269585
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=AAGTTCATGAGATTGCTGCCTT
PRIMER_RIGHT_4_SEQUENCE=TTAGGTCTGCTTGGAAGATGGT
PRIMER_LEFT_4=100,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=60.270
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=136
=
PRIMER_SEQUENCE_ID=SSR_01644 Ps_contig_mira-and-tgicl-ass_franssen_15575
SEQUENCE=TAGTTAGGTCTGCTTGGTAAGGATGGTCTGTAAATAAGTGCCACGGATTCGGCAGATAAGTAGTAGTAGTTAGATTTGTTCTTTGTAATTGCCTCGTAATGTCCGGATTGAGTCACCACAAAGGCAGCAATCTCATGAACTTCTAGACAACCAAAAGAAATCTTCTCTTTATTTGCCTGCTTCTCAATTTGGTGCTTATTGTAATAAGTTTTGACCTGCTCTCTTTTCTCCTCCAATTCTTTACGGAGCTGTTCGTTAGATGCATCCATTCTGGTGTACTTATCATGCAATTCTTCACGATGCCTAGATAAGAAACCA
TARGET=59,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0558
PRIMER_LEFT_PENALTY=0.053515
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=TGGTCTGTAAATAAGTGCCACG
PRIMER_RIGHT_SEQUENCE=GGAGAAAAGAGAGCAGGTCAAA
PRIMER_LEFT=23,22
PRIMER_RIGHT=230,22
PRIMER_LEFT_TM=60.054
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.0870
PRIMER_LEFT_1_PENALTY=0.053515
PRIMER_RIGHT_1_PENALTY=0.033470
PRIMER_LEFT_1_SEQUENCE=TGGTCTGTAAATAAGTGCCACG
PRIMER_RIGHT_1_SEQUENCE=GAATGGATGCATCTAACGAACA
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=270,22
PRIMER_LEFT_1_TM=60.054
PRIMER_RIGHT_1_TM=59.967
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.1218
PRIMER_LEFT_2_PENALTY=0.053515
PRIMER_RIGHT_2_PENALTY=0.068302
PRIMER_LEFT_2_SEQUENCE=TGGTCTGTAAATAAGTGCCACG
PRIMER_RIGHT_2_SEQUENCE=TCCGTAAAGAATTGGAGGAGAA
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=60.054
PRIMER_RIGHT_2_TM=60.068
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.1491
PRIMER_LEFT_3_PENALTY=0.053515
PRIMER_RIGHT_3_PENALTY=0.095629
PRIMER_LEFT_3_SEQUENCE=TGGTCTGTAAATAAGTGCCACG
PRIMER_RIGHT_3_SEQUENCE=CAGAATGGATGCATCTAACGAA
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=272,22
PRIMER_LEFT_3_TM=60.054
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.1826
PRIMER_LEFT_4_PENALTY=0.053515
PRIMER_RIGHT_4_PENALTY=0.129103
PRIMER_LEFT_4_SEQUENCE=TGGTCTGTAAATAAGTGCCACG
PRIMER_RIGHT_4_SEQUENCE=AACAGCTCCGTAAAGAATTGGA
PRIMER_LEFT_4=23,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=60.054
PRIMER_RIGHT_4_TM=60.129
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_01645 Ps_contig_mira-and-tgicl-ass_franssen_15576
SEQUENCE=GSAGGGACCAAACCACACTCCACTCCTTCTTCTTCTTCTCTCACAAACCCTCCCCCTCTCTCTCTCACTTTCTCTCTCTACTCACTTTCTCTCTCTAACCATGTCTACCCACATTGATAACCTCTGGATATTCGCCTTAGCTTCAAAATGCACACAAGAAAACNTTTGTTTACTCTCTCTTGATCATTGGTTTTACTCTGGCCTAACCATGTAATTTTCTTTTACNGGTCTCCGACCCGGGGTTGG
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01646 Ps_contig_mira-and-tgicl-ass_franssen_15576
SEQUENCE=GSAGGGACCAAACCACACTCCACTCCTTCTTCTTCTTCTCTCACAAACCCTCCCCCTCTCTCTCTCACTTTCTCTCTCTACTCACTTTCTCTCTCTAACCATGTCTACCCACATTGATAACCTCTGGATATTCGCCTTAGCTTCAAAATGCACACAAGAAAACNTTTGTTTACTCTCTCTTGATCATTGGTTTTACTCTGGCCTAACCATGTAATTTTCTTTTACNGGTCTCCGACCCGGGGTTGG
TARGET=56,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01647 Ps_contig_mira-and-tgicl-ass_franssen_15581
SEQUENCE=GCTATTTTCCAACAAAATCCAACACCTAACATACAAACCTGTCATTTGACATGCATACCAAACTCTTGTTTATTATATAATATAATATATTCTAATCAGTTCTTCAAAGGTAGAAACTCAATAAACTGGTTTGGCGCATTCATTGCTGAAGCTGATATCTCTGTCTCTTTGTGTTGGTTAATAATGCTATCATCACTGCATCCATTATCTCCTTCGTTCCTTATTGGGAAAAGTTGAAGTGTTTGAGCAG
TARGET=75,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3620
PRIMER_LEFT_PENALTY=1.298798
PRIMER_RIGHT_PENALTY=0.063211
PRIMER_LEFT_SEQUENCE=TGACATGCATACCAAACTCTTG
PRIMER_RIGHT_SEQUENCE=GAACGAAGGAGATAATGGATGC
PRIMER_LEFT=46,22
PRIMER_RIGHT=218,22
PRIMER_LEFT_TM=58.701
PRIMER_RIGHT_TM=59.937
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=1.4262
PRIMER_LEFT_1_PENALTY=1.298798
PRIMER_RIGHT_1_PENALTY=0.127450
PRIMER_LEFT_1_SEQUENCE=TGACATGCATACCAAACTCTTG
PRIMER_RIGHT_1_SEQUENCE=CAACTTTTCCCAATAAGGAACG
PRIMER_LEFT_1=46,22
PRIMER_RIGHT_1=235,22
PRIMER_LEFT_1_TM=58.701
PRIMER_RIGHT_1_TM=59.873
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=1.5001
PRIMER_LEFT_2_PENALTY=1.298798
PRIMER_RIGHT_2_PENALTY=0.201288
PRIMER_LEFT_2_SEQUENCE=TGACATGCATACCAAACTCTTG
PRIMER_RIGHT_2_SEQUENCE=GGAACGAAGGAGATAATGGATG
PRIMER_LEFT_2=46,22
PRIMER_RIGHT_2=219,22
PRIMER_LEFT_2_TM=58.701
PRIMER_RIGHT_2_TM=59.799
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=1.6613
PRIMER_LEFT_3_PENALTY=1.598066
PRIMER_RIGHT_3_PENALTY=0.063211
PRIMER_LEFT_3_SEQUENCE=CCAACAAAATCCAACACCTAAC
PRIMER_RIGHT_3_SEQUENCE=GAACGAAGGAGATAATGGATGC
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=218,22
PRIMER_LEFT_3_TM=58.402
PRIMER_RIGHT_3_TM=59.937
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=1.7255
PRIMER_LEFT_4_PENALTY=1.598066
PRIMER_RIGHT_4_PENALTY=0.127450
PRIMER_LEFT_4_SEQUENCE=CCAACAAAATCCAACACCTAAC
PRIMER_RIGHT_4_SEQUENCE=CAACTTTTCCCAATAAGGAACG
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=58.402
PRIMER_RIGHT_4_TM=59.873
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_01648 Ps_contig_mira-and-tgicl-ass_franssen_15588
SEQUENCE=GTCGGGTTAWGARAAATAWAAGACAAAAGGGTAAGTGGCACAGTTCACTACTCACGGTCGCTTGAAGCTGTTTTTCAATGGGGAAAGTGTGTCATCTTTTTTGGAGAACAGAGACCCTTTTCAATTCAAGTGTAGATCAGTTCAGACCCACAGGAGAAGATCAAGTTACAAAGGTTCTTCTTCTTCTTCTTCCGCTGCTAGATTTCACCATGCATCTTCTTCATTGCTCAGAAGCAAATCAAACTGTTACAATGCTGAAGCACGCCCAAGTGAGGGTGTTCATGATGAATATGGATATGTTTTTGAATATAATGATTATGATGATGATGATGATAGTGAAGATGATGATGAGGATGATGTGTTTGAGGGAGATGGCTTGTCTTTTTTCCGGGGTCTTGTGTTGGATATATCTTATAGGCCAGTCAATGTTGTATGCTGGAAGCGTGCCATTTGTTTAGAGTTCATGGAGAAGGCTGATGTACTAGAGTACTATGATAAGACTGTGAACTCGCCCAGCGGGTCTTTCTATATACCAGCTGTAGTTAAAGGAAATTCGTCATTTACTTCAGGTTAATTAAAAGAAGAAGRGTCTCAAAGAACACTCTTAGTGGAAAAATATTCTGTTTAGAG
TARGET=175,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01649 Ps_contig_mira-and-tgicl-ass_franssen_15588
SEQUENCE=GTCGGGTTAWGARAAATAWAAGACAAAAGGGTAAGTGGCACAGTTCACTACTCACGGTCGCTTGAAGCTGTTTTTCAATGGGGAAAGTGTGTCATCTTTTTTGGAGAACAGAGACCCTTTTCAATTCAAGTGTAGATCAGTTCAGACCCACAGGAGAAGATCAAGTTACAAAGGTTCTTCTTCTTCTTCTTCCGCTGCTAGATTTCACCATGCATCTTCTTCATTGCTCAGAAGCAAATCAAACTGTTACAATGCTGAAGCACGCCCAAGTGAGGGTGTTCATGATGAATATGGATATGTTTTTGAATATAATGATTATGATGATGATGATGATAGTGAAGATGATGATGAGGATGATGTGTTTGAGGGAGATGGCTTGTCTTTTTTCCGGGGTCTTGTGTTGGATATATCTTATAGGCCAGTCAATGTTGTATGCTGGAAGCGTGCCATTTGTTTAGAGTTCATGGAGAAGGCTGATGTACTAGAGTACTATGATAAGACTGTGAACTCGCCCAGCGGGTCTTTCTATATACCAGCTGTAGTTAAAGGAAATTCGTCATTTACTTCAGGTTAATTAAAAGAAGAAGRGTCTCAAAGAACACTCTTAGTGGAAAAATATTCTGTTTAGAG
TARGET=318,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01650 Ps_contig_mira-and-tgicl-ass_franssen_15590
SEQUENCE=ATATATACATAGTACATACATACAGTACTTTTTCTNTTTAATTGTATTTGTTAATTAACTATTAAAAAGTAAAACAAAGACTAATGAATAGCAAAGCAAAGCAACACAAACATAAATAACAAACMATGATGGATCATGGAAAATTACAATCACATTCCTTCCACTGGTTTACATAGCATAAACTATCTCTCCACTGCTATCAATATCGAGGTCAGGATCAGTGTCTAAATCCTCTTCCATGTCATCATCAATATCCAAACTTCCAGTAAGATACTGGTTGTGGCTCTGAGTTCCTGCTGCAGGAACTGTCGCCTCCGCAATTATCTGCATGATTTCTTCGACACTCTGCGTTGATACCACT
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01651 Ps_contig_mira-and-tgicl-ass_franssen_15590
SEQUENCE=ATATATACATAGTACATACATACAGTACTTTTTCTNTTTAATTGTATTTGTTAATTAACTATTAAAAAGTAAAACAAAGACTAATGAATAGCAAAGCAAAGCAACACAAACATAAATAACAAACMATGATGGATCATGGAAAATTACAATCACATTCCTTCCACTGGTTTACATAGCATAAACTATCTCTCCACTGCTATCAATATCGAGGTCAGGATCAGTGTCTAAATCCTCTTCCATGTCATCATCAATATCCAAACTTCCAGTAAGATACTGGTTGTGGCTCTGAGTTCCTGCTGCAGGAACTGTCGCCTCCGCAATTATCTGCATGATTTCTTCGACACTCTGCGTTGATACCACT
TARGET=90,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01652 Ps_contig_mira-and-tgicl-ass_franssen_15592
SEQUENCE=TGACCGTCCATCAAAAAGAAGAGCTTATCCAGTTGTCAACGTGTTATATAGTACTAGTACCTTAAAATAATGGGCTGTCTCATTGCTTAAGAGAGACGAAGAAAACCAAATACTATTGAATTTGTTTCAATACTTTAGCCACTGTGGTGGCGTCTTTGTCTGAACTGAACGCAACAAAAAGAAACCAAACCAAACTCAGAGTTGAGAATCAATTCAAGATATGGGAAGTGTGAGTATGAATCCATTATTTAGAAGTGAGTCTTTTGGATATTATAACTACTTCCCTGACCAGAACTACACCATGATGGAGAAGAGACAGTTGTTCCTAAGAAGCTACCAGTTCTGTAGAAAGAAGAGTCTGACAGAGAGAATCAAAGGGTCATTCATCCGTGCTAAGAAAGTTGTTTGGTTAAAGCTAAGGCATGCTCGTGGATTAAGAAAGTTGGTGTTTTTTCCCAGGTTTAAGTGCGGTTTCTATTACCGTAGAAGAAGGTTCTTTTCACAGCTTTAAACACTAGTAATAATTATAGCCACAAGCGCAAAACTGATTCTTCAACGTGTCTATGGTAGTGAAACATCTATGTAATTCATTCGGATTTAGATTTTAGAACATGTATGTATTTTATTCTGTTCATCGTTCTCTCTATTGTTTAATTGTTTGGTTGATAGGGTTGTTTGTCATATCTTTAACTGCTCCTTCTTTCTTTCTTGTCTTTTCTAATGGAGTGAGCAAATTATTGAAATAATAAAATTAGATTCAKAAATTAGTTTC
TARGET=698,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01653 Ps_contig_mira-and-tgicl-ass_franssen_15599
SEQUENCE=GATACTTGAGAACGTTAACAAAAACCATTCACTTCTCATTCTCTCCTTCAAAAACGCACCCTATTCTTCCCTATTTTGCATCGGCATTTGTTCTTGAATCTCTGTGGTATTCTCTTCACAGAGATGGAAAGAATGTTCCCTACAAAGAAGCCTTCAACTATGAATTCACATGATAGACCCATGTGTGTTCAAGGTGACTCAGGTCTTGTTCTTACCACTGACCCGAAACCTCGTCTTCGTTGGACTGTTGAACTCCATGAACGTTTTGTTGATGCTGTTACTCAACTTGGAGGACCTGACAAAGCCACTCCTAAAACTATTATGAGGGTTATGGGTGTCAAGGGTCTCACTCTTTACCATCTCAAGAGCCATCTTCAGAAATTTAGGCTTGGAAAGCAACCCCACAAAGAATTCAATGATCACTCAATTAAGGATGGTATGAGAGCTTCAGCTTTAGAACTGCAACGAAATACTGCTTCCTCTTCTGCCATGATTGGCCGCAACATGAATGAGATGCAAATTGAGGTGCAAAGAAGACTTCATGAACAGCTTGAGGTGCAAAAACACCTTCAACTAAGGATTGAAGCTCAAGGAAAATACATGCAAAGTATATTAGAGAAGGCTTATCAAACCCTTGCAGGTGAAAACATGGCATCAGCTACAACTAACCTAAAGGGTGTTGTTATTGGACCTCAAGGAATTCCAGAAATGGGACTATTGAAAGAATTTGGTACACCTCTTAGTTTCTCATCTTTTCAAGATCTCGACCTTTTCGGCAGCGGCGGCGGCGATCAACTCGACCTTCAACAAACCATAGGAAAAACCAAACCCTAAGACCATCANGGATTCATCCAAATA
TARGET=779,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0898
PRIMER_LEFT_PENALTY=0.074807
PRIMER_RIGHT_PENALTY=0.014945
PRIMER_LEFT_SEQUENCE=AGGTGCAAAAACACCTTCAACT
PRIMER_RIGHT_SEQUENCE=AGGGTTTGGTTTTTCCTATGGT
PRIMER_LEFT=553,22
PRIMER_RIGHT=831,22
PRIMER_LEFT_TM=60.075
PRIMER_RIGHT_TM=59.985
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.1531
PRIMER_LEFT_1_PENALTY=0.138114
PRIMER_RIGHT_1_PENALTY=0.014945
PRIMER_LEFT_1_SEQUENCE=GAAAACATGGCATCAGCTACAA
PRIMER_RIGHT_1_SEQUENCE=AGGGTTTGGTTTTTCCTATGGT
PRIMER_LEFT_1=642,22
PRIMER_RIGHT_1=831,22
PRIMER_LEFT_1_TM=60.138
PRIMER_RIGHT_1_TM=59.985
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.1636
PRIMER_LEFT_2_PENALTY=0.074807
PRIMER_RIGHT_2_PENALTY=0.088791
PRIMER_LEFT_2_SEQUENCE=AGGTGCAAAAACACCTTCAACT
PRIMER_RIGHT_2_SEQUENCE=GGTCTTAGGGTTTGGTTTTTCC
PRIMER_LEFT_2=553,22
PRIMER_RIGHT_2=837,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=60.089
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.1941
PRIMER_LEFT_3_PENALTY=0.074807
PRIMER_RIGHT_3_PENALTY=0.119274
PRIMER_LEFT_3_SEQUENCE=AGGTGCAAAAACACCTTCAACT
PRIMER_RIGHT_3_SEQUENCE=GAATCCNTGATGGTCTTAGGGT
PRIMER_LEFT_3=553,22
PRIMER_RIGHT_3=848,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.881
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.50
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.1976
PRIMER_LEFT_4_PENALTY=0.182645
PRIMER_RIGHT_4_PENALTY=0.014945
PRIMER_LEFT_4_SEQUENCE=AAGGAATTCCAGAAATGGGACT
PRIMER_RIGHT_4_SEQUENCE=AGGGTTTGGTTTTTCCTATGGT
PRIMER_LEFT_4=694,22
PRIMER_RIGHT_4=831,22
PRIMER_LEFT_4_TM=60.183
PRIMER_RIGHT_4_TM=59.985
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_01654 Ps_contig_mira-and-tgicl-ass_franssen_15616
SEQUENCE=GTCTCCGTTTACATCTTCCTTCCCTCCGTCGTTTGACTACTCCTTTCTCTCTCTACACCTCTCTCTGTCTTTCTCTCTCCTCAAACCTCTCTTATCTCTCTCATTTCTCTCATTCATTCATTCATATCATACCTCATCATATATGTCAATGAATCAATCAATGTGATCGATCTTTCTCACTCTTTCTCTCTTTCTTTTTYACCTTCTTTTCTTCTTCTTAAACCGACAAATCAACCTTACCCTCTTTCTTCTA
TARGET=110,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01655 Ps_contig_mira-and-tgicl-ass_franssen_15619
SEQUENCE=TAGTGCTCATTAGTATCTTTGTTTATTGATGCTGATCATCTGTATTGTGTGGCTTGATTAATCACATTGATTGGATTTGGATAATTTAGGCATATGTCAAGAACTTTGGTGACAAAGATGGGAAGGTTTGGCAGGGTGGTGGAGGAAGAAATCCACCTCCGGAGGCACCTCATCTGGTGGTGACTACTCCTGATATAGAGGCTCTTAGGCCAAAGTCCCCAATGAAGAACCAGCAACAAATGCAGCAGCAGCAGCAATCAGTGTCCATTTCTTTGACGCCGGGTAACGCATCAAACAGAAGGCCTGGGAATTCACCGCAGAATCCAACTGCGCTTGGTGTTAATCAAGATGCCTCTAGCTCAAACCCATCTACACCCGCTCCTTGGGCTTCTTCACCTATGGAGAGCTTTAAGGGACGTGAAGGCTCTGGATTGCCTTCAGATGAATAAAGTATTGATATGTATGGTAGGTTTAATGACCACCACCACCNGGCTATCTAGTATTTTGAATCATTCAGAACAAATAGAATTAGTTGGTTTAGAG
TARGET=242,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4821
PRIMER_LEFT_PENALTY=0.359557
PRIMER_RIGHT_PENALTY=0.122569
PRIMER_LEFT_SEQUENCE=CATCTGGTGGTGACTACTCCTG
PRIMER_RIGHT_SEQUENCE=TAGATGGGTTTGAGCTAGAGGC
PRIMER_LEFT=170,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=59.640
PRIMER_RIGHT_TM=59.877
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.7321
PRIMER_LEFT_1_PENALTY=0.359557
PRIMER_RIGHT_1_PENALTY=0.372557
PRIMER_LEFT_1_SEQUENCE=CATCTGGTGGTGACTACTCCTG
PRIMER_RIGHT_1_SEQUENCE=GGGTGTAGATGGGTTTGAGCTA
PRIMER_LEFT_1=170,22
PRIMER_RIGHT_1=376,22
PRIMER_LEFT_1_TM=59.640
PRIMER_RIGHT_1_TM=60.373
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.8164
PRIMER_LEFT_2_PENALTY=0.359557
PRIMER_RIGHT_2_PENALTY=0.456883
PRIMER_LEFT_2_SEQUENCE=CATCTGGTGGTGACTACTCCTG
PRIMER_RIGHT_2_SEQUENCE=CTTCACGTCCCTTAAAGCTCTC
PRIMER_LEFT_2=170,22
PRIMER_RIGHT_2=422,22
PRIMER_LEFT_2_TM=59.640
PRIMER_RIGHT_2_TM=59.543
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.8757
PRIMER_LEFT_3_PENALTY=0.753123
PRIMER_RIGHT_3_PENALTY=0.122569
PRIMER_LEFT_3_SEQUENCE=ACTTTGGTGACAAAGATGGGAA
PRIMER_RIGHT_3_SEQUENCE=TAGATGGGTTTGAGCTAGAGGC
PRIMER_LEFT_3=102,22
PRIMER_RIGHT_3=371,22
PRIMER_LEFT_3_TM=60.753
PRIMER_RIGHT_3_TM=59.877
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.8757
PRIMER_LEFT_4_PENALTY=0.753123
PRIMER_RIGHT_4_PENALTY=0.122569
PRIMER_LEFT_4_SEQUENCE=AACTTTGGTGACAAAGATGGGA
PRIMER_RIGHT_4_SEQUENCE=TAGATGGGTTTGAGCTAGAGGC
PRIMER_LEFT_4=101,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=60.753
PRIMER_RIGHT_4_TM=59.877
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_01656 Ps_contig_mira-and-tgicl-ass_franssen_15619
SEQUENCE=TAGTGCTCATTAGTATCTTTGTTTATTGATGCTGATCATCTGTATTGTGTGGCTTGATTAATCACATTGATTGGATTTGGATAATTTAGGCATATGTCAAGAACTTTGGTGACAAAGATGGGAAGGTTTGGCAGGGTGGTGGAGGAAGAAATCCACCTCCGGAGGCACCTCATCTGGTGGTGACTACTCCTGATATAGAGGCTCTTAGGCCAAAGTCCCCAATGAAGAACCAGCAACAAATGCAGCAGCAGCAGCAATCAGTGTCCATTTCTTTGACGCCGGGTAACGCATCAAACAGAAGGCCTGGGAATTCACCGCAGAATCCAACTGCGCTTGGTGTTAATCAAGATGCCTCTAGCTCAAACCCATCTACACCCGCTCCTTGGGCTTCTTCACCTATGGAGAGCTTTAAGGGACGTGAAGGCTCTGGATTGCCTTCAGATGAATAAAGTATTGATATGTATGGTAGGTTTAATGACCACCACCACCNGGCTATCTAGTATTTTGAATCATTCAGAACAAATAGAATTAGTTGGTTTAGAG
TARGET=478,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01657 Ps_contig_mira-and-tgicl-ass_franssen_15620
SEQUENCE=GTTAACTTTATATTGCTTTAGAACATTTKACAARATAATRACATAAAATGTAACCAATTTTACAGTAATCCAAACGCTTTCTTTTATTCCCCTAACGAAGCATAATGGCGAGCGCTACCAAAAGAATGAACCGACATTTTTGCAAATAATTTTCTTCCCATTTATATTAGCAGGGAAAAAAAATAATACATAACATAATAACCTTATCCTCAATGTTATGCTACAACATATTCATTTGTCTGAATGATTCAAAATCTAGATAGCCTGGTGGTGGTGGTCCATTAAACCCTCCATACATATCAAATACTTTATCATCTGAAGGCAATCCAGAGCCTTCACGTCCCTTAAAGCTCTCCATAGGTGAAGAAGCCCAAGGAGCGGGTGTAGATGGGTTTGAGCTAGAGGCATCTTGATTAACACCAAGCGCAGTTGGACTCTGCGTTGATACCTACTTGTCTTTGATGCGTTAC
TARGET=266,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01658 Ps_contig_mira-and-tgicl-ass_franssen_15639
SEQUENCE=GGTGTAAWTTATAACTTCTTTCTATATCTTGTGAGTGAGAGAGAGAGAGAATGGTTTCTAAGGTTCACAAGAGAACAGCCATGTACAGAAACTTACAACTGCTACGTTCCACCACATACTCCACTTCCGTGATTATTCTCATACTTGTCTTTCTATTCTCTTTCTGGATGCATGTAACTTTTCTCGACAAATTTCATTTAATTTTCCACCATGATTCATAGCATTTCTTTTCTTAA
TARGET=37,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01659 Ps_contig_mira-and-tgicl-ass_franssen_15640
SEQUENCE=ATATTCAATAACTTTCTGTGCTGTTGCAACTGCAAACAGRTTCAACTCTTCCAGTTTGTGCTTCAAATCTTGTATATATTCCGTTGCTTCCAATATCACCGAAGTTTTCCTTTGCTACATTAAGACGATGAAAGATTCATGATGCATGACAAAATATAGTTCTTCCAAAAATATCTAAACATAGTAGAAAAGATGCTATGAATCATGATGGAAAATTAATGAAATTTGCGAGAAAAGTTCATGTATCCAGAAAGAGAATAGAAAGAGAAGTATGAGAATAATCACGGAAGTGGAGTATGTGATGGAACGTAGCAGTTGTAAGTTTCTGTACATGGCTGTTCTCTTGTGAACCTTAGAAACCATTCTCTCTCTCACTCACAAGATATAGAAAGAAGTTATAATTTACACCCCCGA
TARGET=364,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01660 Ps_contig_mira-and-tgicl-ass_franssen_15659
SEQUENCE=AAAGACAAGTAGGTAATCAAACGCAGAGGAAGGCAAAGACAAATTAGTTTATCATCTAGGATTTCTCATATTCATCTTCACAGTCGAAAGTCTGAAAATAGTCCACCAATGCCTACAACAAATCCATCTCAAACTATTGATTTGGACAAACATGAAGATGGTAATGTTCAAAAAGAAAAAGAAAAGAAAAGGAAAGTTAGAGAAGTTGGTGATTACTAATGATAATGATTCTAATGATGGAGTTCAAGGTAAAACAAAAACCGTATAGGACCTAAATTCGG
TARGET=177,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7344
PRIMER_LEFT_PENALTY=0.216588
PRIMER_RIGHT_PENALTY=1.517784
PRIMER_LEFT_SEQUENCE=AGGTAATCAAACGCAGAGGAAG
PRIMER_RIGHT_SEQUENCE=CCGAATTTAGGTCCTATACGGT
PRIMER_LEFT=10,22
PRIMER_RIGHT=280,22
PRIMER_LEFT_TM=59.783
PRIMER_RIGHT_TM=58.482
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=1.7494
PRIMER_LEFT_1_PENALTY=0.419726
PRIMER_RIGHT_1_PENALTY=1.329720
PRIMER_LEFT_1_SEQUENCE=TAGTCCACCAATGCCTACAACA
PRIMER_RIGHT_1_SEQUENCE=CCGAATTTAGGTCCTATACGGTT
PRIMER_LEFT_1=98,22
PRIMER_RIGHT_1=280,23
PRIMER_LEFT_1_TM=60.420
PRIMER_RIGHT_1_TM=59.670
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=1.8316
PRIMER_LEFT_2_PENALTY=0.501869
PRIMER_RIGHT_2_PENALTY=1.329720
PRIMER_LEFT_2_SEQUENCE=TGCCTACAACAAATCCATCTCA
PRIMER_RIGHT_2_SEQUENCE=CCGAATTTAGGTCCTATACGGTT
PRIMER_LEFT_2=109,22
PRIMER_RIGHT_2=280,23
PRIMER_LEFT_2_TM=60.502
PRIMER_RIGHT_2_TM=59.670
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=1.9375
PRIMER_LEFT_3_PENALTY=0.419726
PRIMER_RIGHT_3_PENALTY=1.517784
PRIMER_LEFT_3_SEQUENCE=TAGTCCACCAATGCCTACAACA
PRIMER_RIGHT_3_SEQUENCE=CCGAATTTAGGTCCTATACGGT
PRIMER_LEFT_3=98,22
PRIMER_RIGHT_3=280,22
PRIMER_LEFT_3_TM=60.420
PRIMER_RIGHT_3_TM=58.482
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=1.9872
PRIMER_LEFT_4_PENALTY=0.657455
PRIMER_RIGHT_4_PENALTY=1.329720
PRIMER_LEFT_4_SEQUENCE=CAATGCCTACAACAAATCCATC
PRIMER_RIGHT_4_SEQUENCE=CCGAATTTAGGTCCTATACGGTT
PRIMER_LEFT_4=106,22
PRIMER_RIGHT_4=280,23
PRIMER_LEFT_4_TM=59.343
PRIMER_RIGHT_4_TM=59.670
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_01661 Ps_contig_mira-and-tgicl-ass_franssen_15664
SEQUENCE=TTGTTTCTGTGAAAGCTATGCGAGAGGCTCCCGAATCCGGTTGGACAATCATTTATAAACTGCAGTAGTGTCTCAGATATGCCACACATTACATTCACAATTGGAAGCAAATTGTTTCCTCTCTCTCCAGAACAGTATATTCTAAAAGTTGATGATGATGATAAAGATTGTTCTCCTGTGTGCTATAGTGGTTTTGTTGCACTAGATGTGCCCTTCCCCACGACGGGTCCCCTAGTTGGGTTTTTGGAGACTATTTTCTTGCAGGCATA
TARGET=150,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5271
PRIMER_LEFT_PENALTY=0.209271
PRIMER_RIGHT_PENALTY=0.317786
PRIMER_LEFT_SEQUENCE=TTCTGTGAAAGCTATGCGAGAG
PRIMER_RIGHT_SEQUENCE=GCACATCTAGTGCAACAAAACC
PRIMER_LEFT=4,22
PRIMER_RIGHT=210,22
PRIMER_LEFT_TM=59.791
PRIMER_RIGHT_TM=59.682
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.5271
PRIMER_LEFT_1_PENALTY=0.209271
PRIMER_RIGHT_1_PENALTY=0.317786
PRIMER_LEFT_1_SEQUENCE=TTCTGTGAAAGCTATGCGAGAG
PRIMER_RIGHT_1_SEQUENCE=GGCACATCTAGTGCAACAAAAC
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=59.791
PRIMER_RIGHT_1_TM=59.682
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=0.7466
PRIMER_LEFT_2_PENALTY=0.428833
PRIMER_RIGHT_2_PENALTY=0.317786
PRIMER_LEFT_2_SEQUENCE=TTGTTTCTGTGAAAGCTATGCG
PRIMER_RIGHT_2_SEQUENCE=GCACATCTAGTGCAACAAAACC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=210,22
PRIMER_LEFT_2_TM=60.429
PRIMER_RIGHT_2_TM=59.682
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.7466
PRIMER_LEFT_3_PENALTY=0.428833
PRIMER_RIGHT_3_PENALTY=0.317786
PRIMER_LEFT_3_SEQUENCE=TTGTTTCTGTGAAAGCTATGCG
PRIMER_RIGHT_3_SEQUENCE=GGCACATCTAGTGCAACAAAAC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=60.429
PRIMER_RIGHT_3_TM=59.682
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.9498
PRIMER_LEFT_4_PENALTY=0.209271
PRIMER_RIGHT_4_PENALTY=0.740565
PRIMER_LEFT_4_SEQUENCE=TTCTGTGAAAGCTATGCGAGAG
PRIMER_RIGHT_4_SEQUENCE=TGCCTGCAAGAAAATAGTCTCC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=59.791
PRIMER_RIGHT_4_TM=60.741
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_01662 Ps_contig_mira-and-tgicl-ass_franssen_15678
SEQUENCE=TCTGACACACGTGTGGGTGTTTGCACGTTTGATGAAACGGAGTTAGTTATTCCTTTTTTTATGATGACTGAAAATTCTGTCTGTACAATTACTTAGAGAATAACGGTAGAATGTAAAAAAGCATTAAACGTAAATAAACTAAATTAGGAATAATTATTGTTAACAAAATTATGTATGTTGCTGCAAAATACGTATATAGAATTGTACAATACTACTCTTATACGTTAACAMWYTTCGCTTATGTTTATTTATTTATTTTGATAG
TARGET=244,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01663 Ps_contig_mira-and-tgicl-ass_franssen_15703
SEQUENCE=ATGGATTTAACCTCTTTTTTTCCTTTCATATTATTATCATTATTATTATTATCCGGTTTTATTTTGTTTATTGAAGTTCTGCTCCGGTATTCTGACTGTTTCCAGAATGGATCATTTTTTTCGATTTTCGGTTTATAGATTCGCCGAGATTCTGGTGTTTCATTGCTTATAGAGTTGTGTTATTGAAACATACAATTTTAGACATATATCTTAACTGTTATATATTTTCTGTTATATATTTTGCATGGTTTGAGAGAACATAATAAAAACATGAAACTGAGTGGTCCTTGGTAATAAAG
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01664 Ps_contig_mira-and-tgicl-ass_franssen_15704
SEQUENCE=AACTTTAGTTTAAGATATATGTCTAAAATTGTATGTTTCAATAACACAACTCTATAAGCAATGAAACACCAGAATCTCGGCGAATCTAATAAAACCGAAAATACGAAAAAAAATGATCCATTCTGGAAACAGTCAGAATACCGGAGCAGAACTTCAAATAAAACAAAATAAAACCGGATAATAATAATAATAATGATAATAATATAGAAAAGGAAAAAAAGAGGTTAAATCCATTGCTGCTTTGTGCCTCCATTCTCTGACAAAA
TARGET=178,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3671
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.364827
PRIMER_LEFT_SEQUENCE=GGAAACAGTCAGAATACCGGAG
PRIMER_RIGHT_SEQUENCE=GCAGCAATGGATTTAACCTCTT
PRIMER_LEFT=124,22
PRIMER_RIGHT=239,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.635
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=0.3671
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.364827
PRIMER_LEFT_1_SEQUENCE=GGAAACAGTCAGAATACCGGAG
PRIMER_RIGHT_1_SEQUENCE=AAGCAGCAATGGATTTAACCTC
PRIMER_LEFT_1=124,22
PRIMER_RIGHT_1=241,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=59.635
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=0.4939
PRIMER_LEFT_2_PENALTY=0.129103
PRIMER_RIGHT_2_PENALTY=0.364827
PRIMER_LEFT_2_SEQUENCE=ACCGGAGCAGAACTTCAAATAA
PRIMER_RIGHT_2_SEQUENCE=AAGCAGCAATGGATTTAACCTC
PRIMER_LEFT_2=139,22
PRIMER_RIGHT_2=241,22
PRIMER_LEFT_2_TM=60.129
PRIMER_RIGHT_2_TM=59.635
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.4939
PRIMER_LEFT_3_PENALTY=0.129103
PRIMER_RIGHT_3_PENALTY=0.364827
PRIMER_LEFT_3_SEQUENCE=AATACCGGAGCAGAACTTCAAA
PRIMER_RIGHT_3_SEQUENCE=AAGCAGCAATGGATTTAACCTC
PRIMER_LEFT_3=136,22
PRIMER_RIGHT_3=241,22
PRIMER_LEFT_3_TM=60.129
PRIMER_RIGHT_3_TM=59.635
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.4939
PRIMER_LEFT_4_PENALTY=0.129103
PRIMER_RIGHT_4_PENALTY=0.364827
PRIMER_LEFT_4_SEQUENCE=ACCGGAGCAGAACTTCAAATAA
PRIMER_RIGHT_4_SEQUENCE=GCAGCAATGGATTTAACCTCTT
PRIMER_LEFT_4=139,22
PRIMER_RIGHT_4=239,22
PRIMER_LEFT_4_TM=60.129
PRIMER_RIGHT_4_TM=59.635
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_01665 Ps_contig_mira-and-tgicl-ass_franssen_15706
SEQUENCE=TTAATTACAATAGTTCAGCTCAAATTTGATACTAGTATATATATACTTTTTGGACTAATACTAGACATTACAAACATGATATTATTGTTGCTATCTCAATTTGGTAGAACTGGAACTGAGAAATATGCCTCTGCCACAGGTGCTTCAATTCCCACCATTCTTGATTCACTAGCAACATCTCCAATGAATCCTTTGTAGAATGCTTTAACCTTAATTACCATGTAAAGAAAAGATGTTGCAACGTAAGAGATTCCCAACCTCTGAAGCTTCTGTGCTTGAGCTAGATTTGTGCTACGTTTTGCACCCTTTTCTTGTGATGATTCTTTCTTATTTTTGTGCTCTTTGAAGGCACTTTCTTCAGGTTGGATTATGCTATTCTCTATTCGACGATAACCATAGTTTTGGCGACGAAATGGTTTTTGTTTGCTTCCTCTTTCACCATAAGAAGCTGCATACTGCATTAGTAATGGTTGGCATCTTACATCACTAACAAGTTGTGAAGCCATGTGTGTGTGAGATATGTGGAGGTTAAGTATTGTCCTCTCTTACTCACTCTTTGTCTCTTAAACTATGTGTC
TARGET=36,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01666 Ps_contig_mira-and-tgicl-ass_franssen_15706
SEQUENCE=TTAATTACAATAGTTCAGCTCAAATTTGATACTAGTATATATATACTTTTTGGACTAATACTAGACATTACAAACATGATATTATTGTTGCTATCTCAATTTGGTAGAACTGGAACTGAGAAATATGCCTCTGCCACAGGTGCTTCAATTCCCACCATTCTTGATTCACTAGCAACATCTCCAATGAATCCTTTGTAGAATGCTTTAACCTTAATTACCATGTAAAGAAAAGATGTTGCAACGTAAGAGATTCCCAACCTCTGAAGCTTCTGTGCTTGAGCTAGATTTGTGCTACGTTTTGCACCCTTTTCTTGTGATGATTCTTTCTTATTTTTGTGCTCTTTGAAGGCACTTTCTTCAGGTTGGATTATGCTATTCTCTATTCGACGATAACCATAGTTTTGGCGACGAAATGGTTTTTGTTTGCTTCCTCTTTCACCATAAGAAGCTGCATACTGCATTAGTAATGGTTGGCATCTTACATCACTAACAAGTTGTGAAGCCATGTGTGTGTGAGATATGTGGAGGTTAAGTATTGTCCTCTCTTACTCACTCTTTGTCTCTTAAACTATGTGTC
TARGET=506,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.1135
PRIMER_LEFT_PENALTY=0.060824
PRIMER_RIGHT_PENALTY=3.052685
PRIMER_LEFT_SEQUENCE=GTGCTCTTTGAAGGCACTTTCT
PRIMER_RIGHT_SEQUENCE=GAGAGGACAATACTTAACCTCCACA
PRIMER_LEFT=335,22
PRIMER_RIGHT=544,25
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=59.947
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=44.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=3.3275
PRIMER_LEFT_1_PENALTY=0.274768
PRIMER_RIGHT_1_PENALTY=3.052685
PRIMER_LEFT_1_SEQUENCE=TTTGAAGGCACTTTCTTCAGGT
PRIMER_RIGHT_1_SEQUENCE=GAGAGGACAATACTTAACCTCCACA
PRIMER_LEFT_1=341,22
PRIMER_RIGHT_1=544,25
PRIMER_LEFT_1_TM=60.275
PRIMER_RIGHT_1_TM=59.947
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=44.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=3.3498
PRIMER_LEFT_2_PENALTY=0.297164
PRIMER_RIGHT_2_PENALTY=3.052685
PRIMER_LEFT_2_SEQUENCE=CTACGTTTTGCACCCTTTTCTT
PRIMER_RIGHT_2_SEQUENCE=GAGAGGACAATACTTAACCTCCACA
PRIMER_LEFT_2=291,22
PRIMER_RIGHT_2=544,25
PRIMER_LEFT_2_TM=59.703
PRIMER_RIGHT_2_TM=59.947
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=44.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=3.4493
PRIMER_LEFT_3_PENALTY=0.396594
PRIMER_RIGHT_3_PENALTY=3.052685
PRIMER_LEFT_3_SEQUENCE=CTTTGAAGGCACTTTCTTCAGG
PRIMER_RIGHT_3_SEQUENCE=GAGAGGACAATACTTAACCTCCACA
PRIMER_LEFT_3=340,22
PRIMER_RIGHT_3=544,25
PRIMER_LEFT_3_TM=60.397
PRIMER_RIGHT_3_TM=59.947
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=44.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=3.5138
PRIMER_LEFT_4_PENALTY=0.461118
PRIMER_RIGHT_4_PENALTY=3.052685
PRIMER_LEFT_4_SEQUENCE=TGAAGCTTCTGTGCTTGAGCTA
PRIMER_RIGHT_4_SEQUENCE=GAGAGGACAATACTTAACCTCCACA
PRIMER_LEFT_4=261,22
PRIMER_RIGHT_4=544,25
PRIMER_LEFT_4_TM=60.461
PRIMER_RIGHT_4_TM=59.947
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=44.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_01667 Ps_contig_mira-and-tgicl-ass_franssen_15707
SEQUENCE=CAAAGGATTCATTGGAGATGTTGCTAGTGAATCAAGAATGGTGGGAATTGAAGCACCTGTGGCAGAGGCATATTTCTCAGTTCCAGTTCTACCAAATTGAGATAGCAACAATAATATCATGTTTGTAATGTCTAGTATTAGTCCAAAAAGTATATATATATTACTAGTATCAAATTTGAGCTGAACTATTGTAATTAATAAGCTTAGATTTGCTAAAATGTTCCCTGCTTTTGCAGTGAGATTACAATTATAGGAAGTGGTTAATTTTTATTTAATGAAGCAATATTGTGTACAAGGGCATTTTCTATTGTACAAGTTCTATCACCATAAGTAAAGTTTGGATATCTAAACTTGTACTAAAG
TARGET=151,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0841
PRIMER_LEFT_PENALTY=0.060196
PRIMER_RIGHT_PENALTY=0.023913
PRIMER_LEFT_SEQUENCE=GTGAATCAAGAATGGTGGGAAT
PRIMER_RIGHT_SEQUENCE=GCAGGGAACATTTTAGCAAATC
PRIMER_LEFT=26,22
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=60.060
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.1597
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.127927
PRIMER_LEFT_1_SEQUENCE=AGGATTCATTGGAGATGTTGCT
PRIMER_RIGHT_1_SEQUENCE=GCAAAAGCAGGGAACATTTTAG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=233,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=60.128
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.1597
PRIMER_LEFT_2_PENALTY=0.031769
PRIMER_RIGHT_2_PENALTY=0.127927
PRIMER_LEFT_2_SEQUENCE=AGGATTCATTGGAGATGTTGCT
PRIMER_RIGHT_2_SEQUENCE=CAAAAGCAGGGAACATTTTAGC
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=232,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=60.128
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.1881
PRIMER_LEFT_3_PENALTY=0.060196
PRIMER_RIGHT_3_PENALTY=0.127927
PRIMER_LEFT_3_SEQUENCE=GTGAATCAAGAATGGTGGGAAT
PRIMER_RIGHT_3_SEQUENCE=GCAAAAGCAGGGAACATTTTAG
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=233,22
PRIMER_LEFT_3_TM=60.060
PRIMER_RIGHT_3_TM=60.128
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.1881
PRIMER_LEFT_4_PENALTY=0.060196
PRIMER_RIGHT_4_PENALTY=0.127927
PRIMER_LEFT_4_SEQUENCE=GTGAATCAAGAATGGTGGGAAT
PRIMER_RIGHT_4_SEQUENCE=CAAAAGCAGGGAACATTTTAGC
PRIMER_LEFT_4=26,22
PRIMER_RIGHT_4=232,22
PRIMER_LEFT_4_TM=60.060
PRIMER_RIGHT_4_TM=60.128
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_01668 Ps_contig_mira-and-tgicl-ass_franssen_15708
SEQUENCE=GTTCGKVGACTTACTAAAATGCACATTCTTCTAAAACTCCTTTTTTGGTTAAGCATTCTCTTCATCAATATGGCCTCTTCTTCTTCTCTCTCATCAGATACCAAACAACAAGAATCTGTTGGTGGTATTGAGGATAAGAAACAAAATCCAGTTCATGAAAATGTTCTTCAGAAACATGCTGCTTTCTTTGATGTTAATAAAGATGGTGTTATTTATCCATGGGAAACTTTCCAAGCAATGCGTGAAATTGGGAGTGGGGTGTTATTATCAACTGCAGCTGCTGCTTTCATTAATGTGGCCCTCAGTCAGACTACTCGTCCTGGGAAGTTCCCATCTCCACTTTTTCCAATTGAAGTTAAGAATATCAAACTAGGCAAACATGGCAGTGACACCGGAGCTTATGATAGTGAAGGAAGGTTTGTTGAATCAAAATTTGAAGAAATTTTCATGAAACATGCACATACGCATCCAAATGCTTTAACACATGATGAGCTCGACGAGTTGATAAAAGCAAACAGAGAGCCTAAAGATCTCACAGGAAGAATTGGTAGCTTTGTTGAATGGAAAATTCTTTACACACTTGCGAAAGACAAGAATGGTTTACTTCCCAAAGAAACAATTCGCAGTGTTTATGATGGAAGCTTGTTTGAAGTCCTCAAAAATGAACATACAAAAAGAATTAGTTCATCCTAATCATAATTTATCTATATAGACGCAAAAAGCACAAGTTATAATATAGACAAATATACCAAAGTTTGTTCACATATGTAATTTACAAAGTATACCAATTTGTTTTGAGTTTCATCGTTCATTTAATGTATCATACTCGCGATGATCTATCATGTTGTGGAATATAGATATTGAAATGACAATTTATATCAAGTTCATGAC
TARGET=76,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01669 Ps_contig_mira-and-tgicl-ass_franssen_15711
SEQUENCE=ACTTTACTCCTCTCAAATCTAAAGTCTCCCCTTTTCTTATCATCCAAATTAAAAAGCTCATAGGACAAACATTCATAAATCCCACAGAAAAAACTCACATATAGGTAGAAAAAATAAAACAACAACATTATCAACTAGTGAAATGAAATGCAACTTCAAATCCAACTATTGAGTGACACGATACCTATCCAATGGATCCTTGAACAACAGACATTCATGATAAACTCTCCAAGCATAGTCCTCAAAATCAATGTTGTTGAATACCTTCTTTTKGACTTCACCACCAATGTCGCTTTCTTCTTCATCTGCGTCTTCAACTTTGTCTTTGATGCCAATGGGAAGAAGTGGAGCTACATTGCTCTCTGTAGGAAGAGTTATCAACAATGACATTGTTATGCAACGTGAATCGTTTTCATCTCCCAATGTTGCCATTGGTCTGCCTCTCACTTTCTTTAGCTTTCCATTGCTCCACACCTGAGCAATATCACCAACAGTAACTAAGATGGAGTCCACGTGTGGTAATACAGAAATCCATCCACTTGAATCCGACAACAAAGAGTGCTTCTGGCACCTTATCTGATACTGCAACCCAATAATAAACGGGTAAAACCCACTTCTTGAACCGGGTTTATTTCCGGCTAAACACTCCGAAATCCACATAATCGAACTGAACCGGGTTGGGTCCTCTTCAACCGGATTCACAACTCCTAAACCCTTCGTCAACACATCAATGATATTCAAACCCACCTTCTCAAGCGCGCGTGCGAACTCGTTCAGGGAATCCAGTTTTAACTCGTCCGAGACTGTTGAGCACGACAACGCCAACCGGAACGACTCGGTGATTCCGTCATCGTCGTCATTATCGCCGCCTTCGTAACCTAAAGGCCAGTTTTGAGGGAAGAACGATTCCTTTTGGTCACGAGAAAGGTCGAATAGTTTAAGCGATTCCGACTCGGCCGAGTTGGCGAGTTTGGAGGAAACCGAGTGATCGGTTAGTTGAACGAAACCGGACTTGGAAACAGAAGAGAGAAGTTCGTTTGGAGTTAATGAAGAAAAAGAGAGAGACGGAGGAGACGTGGAGGATGAAGGAGAGCGGCGGTTCGGGAGAGAGAGATTTGGCGGGAGACGGTGGAGGAGACGTGAGAGTGCGTCGGCGGCATCGGAAGATGAGAGGAGGTTGCTGGTGTTATTTGACGGCGTTGGTGGTGGTGCTGACGTGGCACCACCGTGAAGGTTTGATAATAGTTG
TARGET=1105,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01670 Ps_contig_mira-and-tgicl-ass_franssen_15713
SEQUENCE=GGCCTCGGGCGTCGCTCCTATAGATGATGGTGGTTTGGTCTAGCTGTATTCTTTCCTTCCATTCTTCAACCTTCCATCTGTATCTAGAGGTACCACAGGAACTAGACTCCAAATGAGGATTAGGAGACCTTCGAGGCTCTGGGGGGAAAATTACGAGGCAGAATCTGGATCTGCTACTAGAGATAGAGATGATACAACAAATGAGTCTTCCGATGGAGGCTCTGATAATAGGAGGCGCCGTGTTAGAAGACGGATTCAAACACCTGACAGACAACCATAACTTGGAAAACCAGATATGTCAATTTGAATTGTTGATAATGTTCCCAAGTAATAGGTGGAAGAGCAAGAGAGAGAGAGAGAGTGGGGAGTTTCCGGCTTGGATATTCAAATCTGCTCTAGCAAGTGTTGAACAGTTATTGCTAATTCTCAGAATGAGAAGGGTGCTATTCGTTGAGCATCCCATAATCTGTTTATCTAACTATTTTTAGCATTCCTGCATCTTTGGTGCCATATCTCTAGAACAATTGAGTTTGAAATAAATTTACTGTTGTGTTTCTTTTTCTCTCTACAATCTATATGTGAGGCATCATTGAAAGAAAATGCCATGTGAGGTGAAGTATTGACCTGCCTTGTTACTATAGTTGTTTAGCTTAGAAAATTAATTTTATAACGTAAAAAAAG
TARGET=346,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1903
PRIMER_LEFT_PENALTY=0.012470
PRIMER_RIGHT_PENALTY=0.177853
PRIMER_LEFT_SEQUENCE=GGATTCAAACACCTGACAGACA
PRIMER_RIGHT_SEQUENCE=GATTATGGGATGCTCAACGAAT
PRIMER_LEFT=251,22
PRIMER_RIGHT=466,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.178
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=0.2286
PRIMER_LEFT_1_PENALTY=0.050763
PRIMER_RIGHT_1_PENALTY=0.177853
PRIMER_LEFT_1_SEQUENCE=TTCAAACACCTGACAGACAACC
PRIMER_RIGHT_1_SEQUENCE=GATTATGGGATGCTCAACGAAT
PRIMER_LEFT_1=254,22
PRIMER_RIGHT_1=466,22
PRIMER_LEFT_1_TM=60.051
PRIMER_RIGHT_1_TM=60.178
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.2476
PRIMER_LEFT_2_PENALTY=0.069737
PRIMER_RIGHT_2_PENALTY=0.177853
PRIMER_LEFT_2_SEQUENCE=CAAACACCTGACAGACAACCAT
PRIMER_RIGHT_2_SEQUENCE=GATTATGGGATGCTCAACGAAT
PRIMER_LEFT_2=256,22
PRIMER_RIGHT_2=466,22
PRIMER_LEFT_2_TM=59.930
PRIMER_RIGHT_2_TM=60.178
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.2529
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.177853
PRIMER_LEFT_3_SEQUENCE=CGATGGAGGCTCTGATAATAGG
PRIMER_RIGHT_3_SEQUENCE=GATTATGGGATGCTCAACGAAT
PRIMER_LEFT_3=210,22
PRIMER_RIGHT_3=466,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=60.178
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=8.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.2658
PRIMER_LEFT_4_PENALTY=0.012470
PRIMER_RIGHT_4_PENALTY=0.253332
PRIMER_LEFT_4_SEQUENCE=GGATTCAAACACCTGACAGACA
PRIMER_RIGHT_4_SEQUENCE=CGAATAGCACCCTTCTCATTCT
PRIMER_LEFT_4=251,22
PRIMER_RIGHT_4=449,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.747
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_01671 Ps_contig_mira-and-tgicl-ass_franssen_15720
SEQUENCE=CGTACAGTGGTATACAAACGACAGAGTACGGSGGGAACTCACATTTGTTCATATCTTCAAAAAGAAAAACATTGTAAAGTCTCAAATTTAAATATTGAGTTTTGTGTGTGTGTTTGTGGTTGTGTGTGAGAGAGAGAGGGAACAAAAAGAGAAGAGTAATGGTGGAAAAGTGGAAGAAAGGTGTTTTGGTAGGGAAAAAGGGAGGAACATGTACTACTCCATCACCTACATGGAGATTTGAACCTCCATCTCAACAGAATAATGTTGTTTCAACTTCAACCGTTTCTGCTAGAAAGCTTTGTGCCAAT
TARGET=103,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01672 Ps_contig_mira-and-tgicl-ass_franssen_15720
SEQUENCE=CGTACAGTGGTATACAAACGACAGAGTACGGSGGGAACTCACATTTGTTCATATCTTCAAAAAGAAAAACATTGTAAAGTCTCAAATTTAAATATTGAGTTTTGTGTGTGTGTTTGTGGTTGTGTGTGAGAGAGAGAGGGAACAAAAAGAGAAGAGTAATGGTGGAAAAGTGGAAGAAAGGTGTTTTGGTAGGGAAAAAGGGAGGAACATGTACTACTCCATCACCTACATGGAGATTTGAACCTCCATCTCAACAGAATAATGTTGTTTCAACTTCAACCGTTTCTGCTAGAAAGCTTTGTGCCAAT
TARGET=128,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01673 Ps_contig_mira-and-tgicl-ass_franssen_15721
SEQUENCE=AACTGTGCCGCAGCCTGGTCAGAGGAGTTGTTTTTGCGGCGGAGAGTGGCGCCACCATGTTGAATTTGCCAGAGATTGGCAACAAAGCTTTCTAGCAGAAACGGTTGAAGTTGAAACAACATTATTCTGTTGAGACGGAGGTTCAAATCTCCATGTAGGTGATGGAGTAGTACATGTTCCTCCTTTTCCACCAAACNCCTTTCTTCCACTTTTCCACCATTACTCTTCTCTTTTGTCCCTCTCTCTCTCACACACAACCACAAA
TARGET=239,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01674 Ps_contig_mira-and-tgicl-ass_franssen_15726
SEQUENCE=TTTGGCTCTCTTTTCTCTTCAAAACCATCTCTCAAATTCTCAAAGACACAGAGAAACAACCCAGGTGTGTTGAGATAATGCTGAGGAAGAGAAGCTAGTTWSTTMAWWTCCAGAAAGATCAACACAACATGGGTTATTTGACAAATTCTGATGCTAATTCTGAAAAGTATTTGAAATCTCATGATTTCGGTCGCAATATCAAAGGCAACTCGATTTTCAATATCCCTTGTTTGTTTGTGGGTTTAGGTCCAAAAGGGTTTTTGGATTCGGATTCGGTTAGAAGTCCAACTTCACCTTTAGATACTAAAGTTCTTTCGAATTTTGGTAACCATGTTAGGAACCAAAGATCTTCGCTTTTTGAGGGGAATCAAAGGAGTTGGAATTGTTGCAAAGTTGGTTTAAGTATTGTAGAGTCTTTGGAAGATTGTAACTGTTCTAGGTTTTGTGGGAAAACTCTTA
TARGET=227,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01675 Ps_contig_mira-and-tgicl-ass_franssen_15727
SEQUENCE=TTATAACAACTTTWRGTCGAAACAAAAATAAGACTGGGTTTACKAATAAAAAAATTAACTTCTAATAAATAAAAAACTAATAATACACATCCATATTGATCTATACACAAATAAAATCAAAGAGACCACAAATAAACTAATCTCGCTTCTCCCTTTAGATTTGTCTATCCCTTGAATAGAATAAGACAAGGAAGTGCAAGATTGGCACCAAAGAAATAAAATAAATATTGTCTGGATCATAAATATTATAACTCAAAGATGATATATCCAGACATTTATTTAACGAATTTAGACATGAAGCTAGAAGTTTCACTTTGCTCCAAATGGAGATTGACTTTATTCCTAGATGAAAACTGGAACATGATTTATCATGAATGGTGCTTCTGCAGTTTGAGCTTAGTTGTAGATGGGTGGTGGTGGTGAAGCATAAATATAAGCTGGTGGTGGAGGTGCCCGGTATCTGCGTTAG
TARGET=410,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01676 Ps_contig_mira-and-tgicl-ass_franssen_15728
SEQUENCE=ATATCCAGACATTATTTTAACGTAAATTTAGTACATGAAGCTGAAGTTTCACTTTGTCTCCAAATGGTAGATTGACTTATTCCTAGATGAAAACTGGAACATGATTTATCATGAATGGTGCTTCTGCAGTTTGAGCTTAGTTGTAGATGGGTGGTGGTGGTGAAGCATAAATATAAGCTGGTGGTGGAGGTGACTTTACCGGTGGGGGAGGACTCACATATTGGTAAGGAGGTGGAGGAGACGACGATGGTGGTGGAGGGGATTTGTAGTAGTAGGGAGGATGAGAAATGGGAGATGGTGGAGGAGGACTTTGATAGTGATAAGGAGGTGGTGGTGATGGAGATGGAGGTGGGGGAGATTTGTAAGGAATAGAAGAGAAGAGAATGAGAGTC
TARGET=150,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1120
PRIMER_LEFT_PENALTY=0.037486
PRIMER_RIGHT_PENALTY=0.074466
PRIMER_LEFT_SEQUENCE=TGCTTCTGCAGTTTGAGCTTAG
PRIMER_RIGHT_SEQUENCE=TACCAATATGTGAGTCCTCCCC
PRIMER_LEFT=118,22
PRIMER_RIGHT=225,22
PRIMER_LEFT_TM=59.963
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=108
PRIMER_PAIR_PENALTY_1=0.1470
PRIMER_LEFT_1_PENALTY=0.037486
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=TGCTTCTGCAGTTTGAGCTTAG
PRIMER_RIGHT_1_SEQUENCE=TACTACAAATCCCCTCCACCAC
PRIMER_LEFT_1=118,22
PRIMER_RIGHT_1=270,22
PRIMER_LEFT_1_TM=59.963
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=153
PRIMER_PAIR_PENALTY_2=0.1549
PRIMER_LEFT_2_PENALTY=0.037486
PRIMER_RIGHT_2_PENALTY=0.117452
PRIMER_LEFT_2_SEQUENCE=TGCTTCTGCAGTTTGAGCTTAG
PRIMER_RIGHT_2_SEQUENCE=ATCACCACCACCTCCTTATCAC
PRIMER_LEFT_2=118,22
PRIMER_RIGHT_2=337,22
PRIMER_LEFT_2_TM=59.963
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.1773
PRIMER_LEFT_3_PENALTY=0.102789
PRIMER_RIGHT_3_PENALTY=0.074466
PRIMER_LEFT_3_SEQUENCE=TTATCATGAATGGTGCTTCTGC
PRIMER_RIGHT_3_SEQUENCE=TACCAATATGTGAGTCCTCCCC
PRIMER_LEFT_3=105,22
PRIMER_RIGHT_3=225,22
PRIMER_LEFT_3_TM=60.103
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=121
PRIMER_PAIR_PENALTY_4=0.2108
PRIMER_LEFT_4_PENALTY=0.037486
PRIMER_RIGHT_4_PENALTY=0.173354
PRIMER_LEFT_4_SEQUENCE=TGCTTCTGCAGTTTGAGCTTAG
PRIMER_RIGHT_4_SEQUENCE=TATCAAAGTCCTCCTCCACCAT
PRIMER_LEFT_4=118,22
PRIMER_RIGHT_4=315,22
PRIMER_LEFT_4_TM=59.963
PRIMER_RIGHT_4_TM=59.827
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=198
=
PRIMER_SEQUENCE_ID=SSR_01677 Ps_contig_mira-and-tgicl-ass_franssen_15736
SEQUENCE=GAATTGAATTTTGGTTTTAGCCAAGCTCGATCCAAACAATTTATGTACACTCTAATCTAATGTAAATGTAAATCATAAATGTCAATTAAAAAAATATTAGTGTTAACGGAGGTATTKAATTGTATGTAATTGTAATGGATAGATAGTTGACGAGAGTAAACCACTCCATAGGTCATCATCCATATACCTACGCTAACAAACAACAATCACAACGCAATGGACATCAATGCGGATGATCGGGACGACTCAAAACCGGTGGTCCTCGTCACGGGTTGCAGCGGCGGAGGAATCGGCCACGCGCTTGCACGCTCCTTCGCCGCCAACAGTTGCAAAGTAGTGGCCACCAGCCGATCGCGTTCCACCATGGCGGACTTGGACCACGACCCCAAATTCTTCCTTCAAGAACTCGATGTTCAGTCCGATGAGAGCGTAAACAGAGTTGTCAATACTGTCTTGAATAAATACAGCCGTATTGATATTCTCGTCAATAACGCCGGTGTTCCGTGTGTCGGTCCACTCGCCGAGCTTCCTCTATCGACTATTCAAAACACCTTTGATACTAATGTCTTTGGTTCTTTGAGAATGGTCCAGGCGGTGGTTCCTCACATGGCAGCTAGGAAGCAGGGAAAGATTGTGAATATTGGCAGTGTTACTGGATTGGCCTCTGGACCTTGGGCGGGTGCTTACAGTGCTTCTAAGGCTGCCATTCATGCTTTGACAGATACATTAAGATTGGAACTTGGTCATTTTGGAATTGATGTTGTGAACATTGTCCCTGGAGCTGTGAAGTCGAATATTGGAAATTCTGGCATATCCATCTACAACAGCATGCCAGAGTGGAAACTGTTTAAGCCATTTGAAGCAGCAATCAGAGAGAGAGCTCTACTTTCTCAGAGGTCAAGATCAACCCCTACAGATGAGTTTGCTAAACATACTGTAGCTGCTGTCCTTAGAAAGAAACCACCTGCATGGTTCTCTTATGGTCATTATACCACTGCCATGGCTATCATGTACCATTTGCCTCTTTGTGTTAGAGACTTTGTTTTCAAGAAAGCAATGAAATGCTGAGCTATACTTCATCTACCCATCTTTATGGAGATCTCAATCAATAAATTGCTAGTATTCCGCTTAAGCAGTAATGGTGTACTGTACTGCATGATTATTATGCATGGTTGCTAATCAATTGTGTGTCCCAGTCAAAAAAAAGTATTTTCTTTACTATTTTGATAATTTATATTAGGAGGTGTAACGGTGGGGTTCGGGGTTGTAGTTTGCCAAATCCAACTACCGGTTACGTTCGGGGTTTTGGGTA
TARGET=871,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01678 Ps_contig_mira-and-tgicl-ass_franssen_15771
SEQUENCE=TATCTTGGTTGTTTAATTAAAGTTACATAAAAAAAATAGTTTTGTAAAACAACTTTTAATGTCTACTTTGGAGCATGTTATTATTAGACAAATACATAATGAATTTAAAGTGAAAAATACTGCAAGGTCTATAGTAGAGCAAATCATCAAAAGAGTCCCAAATACTATTGATCATTTTACAGCAGAATAATCAAGAAAAAGATGAGACTCATTCAAACACGCAGACAGACAGAATGAAAATCATAGGATTCTTATCTCAAGCAAAGCATGGTTGTTGTTGCTGCTATTCCTCTTCCCACATTCTTGTTCTCATCATCACACACTTTCAATTTTCGAATACTCAAAGTTAAAGCTAATGCTACACTTATCACAAACTCTGATTGGTTTCAAGTGGGTAGACCCATTGGGAACTATGGTTTCATGAACGTTACCACTAATACTGATCAATATTCGTTTGGTGAAGGAGGAGGATTCAAAAGTCAAGATGTTCAAGAAGGTAGCGTCAAAATCAGGCTTTATGAAGGCAGGGTTTCTCAAGGTCCGCTTACACAAACACCTGTTCTCTTTAAGGTTTATCCCGGAACACGAGCTGGTGGTGTTGTGGCAGATATGATGGCTGCCAACGAGTTGAATTCCCACATGTTCCTTCAAAGTAGTTCTAAAGGTATGAGTCAGCATCTTATGTTACTTTTAGGTGGCTTTGAAACAACCACCGGAGAGCAGTGGCTTGCTTTTCGTGATTACGGGAAATCTAGCGCAGCAGACTATGCTAAAGTGGCGAGTGACAAGATATCAAAACTGTCTTCGTGGAATAGCTTTGAACGAGGACAGGCAATGAAAAGAAGACGACGTTTCATTATAATACTGCTTCAGGGTGCTTTGAGAGGTTTAGCTTACATGCATGATCATGATAGATTGCACCAGAGTCTTGGACCGTTTTCCGTATCTCTCAACACAATTTCTGAAACAGATGCTCCCTATTTGATTTCAAGGCTTAAGGATTTAGCCTTTTCTGTTAGTGTTAGGTACTCAGAACTAGAGGATTCGGGACCACTTACGGAAGGTCTTTGGGCACGAGCTTCTGCAGCTAGTGCATTCACTTATTTAGAGAAAAGAGCTTTTGGAATAGCCGATGACATATATGAGGCAGGCCTTCTTTTTGCATACTTGGCTTTTGTTCCGTTTTGTGAAGCTGGTGTAATGGATGGCCTTTCTTTGCAAAGGCTTTTAGAGAACACTTTTCGGCTTGATCTTGAAGCAATGCGAGAGTAAGATATCTTTTATTCTATATTTCTGGTTTAACTATTACTGGGAGTAGACATCTGTTGTTTTAAAGCTAGTTTTCCATGGTTGAGCTATACTTTACAAAGTGTGAGTGGATTCAAAAGTTCCGTTGTCTTGTTGCAAGTTGCATCCACTCCAAAAATTAAAAAAGGAAGTATATTTTTCACAAACTAAAATTTGCATTTTATTCTAGCATGTGTAAAACTCTGAAGAAGCAAATGCCTAGTAATACACAAGATCAATCAAATTACAAAAGAAAATAAAGGTTATACAGAAATTGGATA
TARGET=222,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4253
PRIMER_LEFT_PENALTY=0.401769
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=AGCAAATCATCAAAAGAGTCCC
PRIMER_RIGHT_SEQUENCE=AACAAGAATGTGGGAAGAGGAA
PRIMER_LEFT=137,22
PRIMER_RIGHT=307,22
PRIMER_LEFT_TM=59.598
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=0.6661
PRIMER_LEFT_1_PENALTY=0.401769
PRIMER_RIGHT_1_PENALTY=0.264300
PRIMER_LEFT_1_SEQUENCE=AGCAAATCATCAAAAGAGTCCC
PRIMER_RIGHT_1_SEQUENCE=CAACCATGCTTTGCTTGAGATA
PRIMER_LEFT_1=137,22
PRIMER_RIGHT_1=273,22
PRIMER_LEFT_1_TM=59.598
PRIMER_RIGHT_1_TM=60.264
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=0.6906
PRIMER_LEFT_2_PENALTY=0.401769
PRIMER_RIGHT_2_PENALTY=0.288804
PRIMER_LEFT_2_SEQUENCE=AGCAAATCATCAAAAGAGTCCC
PRIMER_RIGHT_2_SEQUENCE=TGAGAACAAGAATGTGGGAAGA
PRIMER_LEFT_2=137,22
PRIMER_RIGHT_2=311,22
PRIMER_LEFT_2_TM=59.598
PRIMER_RIGHT_2_TM=59.711
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=175
PRIMER_PAIR_PENALTY_3=0.7860
PRIMER_LEFT_3_PENALTY=0.401769
PRIMER_RIGHT_3_PENALTY=0.384229
PRIMER_LEFT_3_SEQUENCE=AGCAAATCATCAAAAGAGTCCC
PRIMER_RIGHT_3_SEQUENCE=AGAACAAGAATGTGGGAAGAGG
PRIMER_LEFT_3=137,22
PRIMER_RIGHT_3=309,22
PRIMER_LEFT_3_TM=59.598
PRIMER_RIGHT_3_TM=59.616
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=173
PRIMER_PAIR_PENALTY_4=0.7869
PRIMER_LEFT_4_PENALTY=0.401769
PRIMER_RIGHT_4_PENALTY=0.385098
PRIMER_LEFT_4_SEQUENCE=AGCAAATCATCAAAAGAGTCCC
PRIMER_RIGHT_4_SEQUENCE=AATGTGGGAAGAGGAATAGCAG
PRIMER_LEFT_4=137,22
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=59.598
PRIMER_RIGHT_4_TM=59.615
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_01679 Ps_contig_mira-and-tgicl-ass_franssen_15775
SEQUENCE=AGTTCAATTATTTTATTGTACCACATGCTGAATATTAAATACTATACAAYTTATAAATATTAAATTATACAATAACTAATAATATTAATTGGCTAATACAAATCAAAAGCAAAAAAAAAATTAATGGGAATTAATTAATTTACAATTGAATTACTCATGAATATAGAACCAATGGAGACTCYTTTTCTTCAATCTAAAAAATACATATAAATATAAGTTAAACAAAAGAACAAGCTAAAGAACTTGTTTGCAGTAAAGAGCAGTTTCAATGCATGCTCAATCCTTAANCCTATTGCAGTACACTTCNGAGAACATGG
TARGET=129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01680 Ps_contig_mira-and-tgicl-ass_franssen_15776
SEQUENCE=ATTTGTATTTGCTGGCTCCTCCAAATCTTTCTGATACCCTTTTCAAATATATAGCTCCTGAGCAAGTGCCAGTTCAATATGGAGGACTTAGCAGAGAAGGCGAACAGGAATTCACCACGGCTGACCCTGCTACAGAGGTTATTATCAAACCAGCAACAAAACATGCTGTTGAGTTCCCAATTTCTGAGATGTGGATCTCAGATTTTGGGGTATATTAGTATTAATTATAGTTCTCCAACACTTGTTAAGGATGGAAAAGTTAGCTATAAAGAGTAAAGTAGCAATGCTAAGGTAATGGGAAGCAAATAGGATTAAAGGATTGAGCATTGCATTTGAAACTGCTCTTTACTGCAAACAAGTTCTTTAGCTTGTTCTTTTTGTTTAACTTATATTTATATGTATTTTTTTAGATTGAAGAAAAAGAGTCTCCATTGGTTCTATATTGATGAGTAATTCAATTGTAAATTAATTAATTCCCATTAATTTTTTTTTKKCTTTTGTATTTGTTATTAGCCAATTAATATTATTAGTTATTGTATTAATTTAAATTAATTT
TARGET=464,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01681 Ps_contig_mira-and-tgicl-ass_franssen_15778
SEQUENCE=CGTAACGACAGAGTACGCGGGGTCGTCTCTCAGCATTCTATTTCTACTCACTGCTCTCTCTCTCTCACTCTCTCATATTTCCATTTCAACTTCTTCTGAAGCTCCTTCTTTCCAGATCGAGCTTCGGAAACAAAAATAAATTATTAATTTAGATTCAGTTTTCATTTTCTTCATTCATGGCAACAAGTTCGTTACATCTTCAATACTACGGAGTGAACGTGAAACCGAATCTTCTACCAGTGACGTTGCCGCTGAGAACGAAGCTTTTTATCAACAATCATGAAACGACGCCGTCGTCGTCGTTGAAGGTCACATGCAAGTCTTCGAGCGACGTGTTGGAGAAGCACGTGATCGAGAATGGGAAGCACGTGACGAGTGAAGATGAAAACAGAAAGAGTTACACTTGTGTGATGAAATTCGGTGGCTCTTCAGTTGCTTCTGCGGAGAGGATCATGGAAGTTGCTACTCTTGTTATGAGTTTTCCTCAAGAGATTCCTATTGTTGTTCTTTCTGCCATGGGAAAAACCACTAACAAGCTTTTGCTGGCTGGAGAAAAAGCTGTGAGTTGCGGTGTTACTAATGTCTCTTGTATTGAAGAGCTTAGCTTTATAAAAGATCTGCATCATAGGACTGTGGATCAGCTTGGAGTGGACAGATCTGTTATTGAGAAGCACCTCGAAGAATTGGAG
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2985
PRIMER_LEFT_PENALTY=0.259289
PRIMER_RIGHT_PENALTY=0.039178
PRIMER_LEFT_SEQUENCE=GGGTCGTCTCTCAGCATTCTAT
PRIMER_RIGHT_SEQUENCE=GTAACGAACTTGTTGCCATGAA
PRIMER_LEFT=19,22
PRIMER_RIGHT=194,22
PRIMER_LEFT_TM=59.741
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.2992
PRIMER_LEFT_1_PENALTY=0.259289
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=GGGTCGTCTCTCAGCATTCTAT
PRIMER_RIGHT_1_SEQUENCE=AAGATTCGGTTTCACGTTCACT
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=59.741
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=0.3024
PRIMER_LEFT_2_PENALTY=0.259289
PRIMER_RIGHT_2_PENALTY=0.043121
PRIMER_LEFT_2_SEQUENCE=GGGTCGTCTCTCAGCATTCTAT
PRIMER_RIGHT_2_SEQUENCE=ATAAAAAGCTTCGTTCTCAGCG
PRIMER_LEFT_2=19,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=59.741
PRIMER_RIGHT_2_TM=60.043
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=0.5826
PRIMER_LEFT_3_PENALTY=0.259289
PRIMER_RIGHT_3_PENALTY=0.323355
PRIMER_LEFT_3_SEQUENCE=GGGTCGTCTCTCAGCATTCTAT
PRIMER_RIGHT_3_SEQUENCE=AGATGTAACGAACTTGTTGCCA
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=198,22
PRIMER_LEFT_3_TM=59.741
PRIMER_RIGHT_3_TM=59.677
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.7963
PRIMER_LEFT_4_PENALTY=0.259289
PRIMER_RIGHT_4_PENALTY=0.536975
PRIMER_LEFT_4_SEQUENCE=GGGTCGTCTCTCAGCATTCTAT
PRIMER_RIGHT_4_SEQUENCE=AGATTCGGTTTCACGTTCACTC
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=231,22
PRIMER_LEFT_4_TM=59.741
PRIMER_RIGHT_4_TM=60.537
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_01682 Ps_contig_mira-and-tgicl-ass_franssen_15778
SEQUENCE=CGTAACGACAGAGTACGCGGGGTCGTCTCTCAGCATTCTATTTCTACTCACTGCTCTCTCTCTCTCACTCTCTCATATTTCCATTTCAACTTCTTCTGAAGCTCCTTCTTTCCAGATCGAGCTTCGGAAACAAAAATAAATTATTAATTTAGATTCAGTTTTCATTTTCTTCATTCATGGCAACAAGTTCGTTACATCTTCAATACTACGGAGTGAACGTGAAACCGAATCTTCTACCAGTGACGTTGCCGCTGAGAACGAAGCTTTTTATCAACAATCATGAAACGACGCCGTCGTCGTCGTTGAAGGTCACATGCAAGTCTTCGAGCGACGTGTTGGAGAAGCACGTGATCGAGAATGGGAAGCACGTGACGAGTGAAGATGAAAACAGAAAGAGTTACACTTGTGTGATGAAATTCGGTGGCTCTTCAGTTGCTTCTGCGGAGAGGATCATGGAAGTTGCTACTCTTGTTATGAGTTTTCCTCAAGAGATTCCTATTGTTGTTCTTTCTGCCATGGGAAAAACCACTAACAAGCTTTTGCTGGCTGGAGAAAAAGCTGTGAGTTGCGGTGTTACTAATGTCTCTTGTATTGAAGAGCTTAGCTTTATAAAAGATCTGCATCATAGGACTGTGGATCAGCTTGGAGTGGACAGATCTGTTATTGAGAAGCACCTCGAAGAATTGGAG
TARGET=292,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1420
PRIMER_LEFT_PENALTY=0.039879
PRIMER_RIGHT_PENALTY=0.102129
PRIMER_LEFT_SEQUENCE=AGTGAACGTGAAACCGAATCTT
PRIMER_RIGHT_SEQUENCE=CACCGAATTTCATCACACAAGT
PRIMER_LEFT=211,22
PRIMER_RIGHT=422,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.4255
PRIMER_LEFT_1_PENALTY=0.323355
PRIMER_RIGHT_1_PENALTY=0.102129
PRIMER_LEFT_1_SEQUENCE=TGGCAACAAGTTCGTTACATCT
PRIMER_RIGHT_1_SEQUENCE=CACCGAATTTCATCACACAAGT
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=422,22
PRIMER_LEFT_1_TM=59.677
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=0.4875
PRIMER_LEFT_2_PENALTY=0.385416
PRIMER_RIGHT_2_PENALTY=0.102129
PRIMER_LEFT_2_SEQUENCE=CTTCATTCATGGCAACAAGTTC
PRIMER_RIGHT_2_SEQUENCE=CACCGAATTTCATCACACAAGT
PRIMER_LEFT_2=168,22
PRIMER_RIGHT_2=422,22
PRIMER_LEFT_2_TM=59.615
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=0.5582
PRIMER_LEFT_3_PENALTY=0.456098
PRIMER_RIGHT_3_PENALTY=0.102129
PRIMER_LEFT_3_SEQUENCE=ACTACGGAGTGAACGTGAAACC
PRIMER_RIGHT_3_SEQUENCE=CACCGAATTTCATCACACAAGT
PRIMER_LEFT_3=204,22
PRIMER_RIGHT_3=422,22
PRIMER_LEFT_3_TM=60.456
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=219
PRIMER_PAIR_PENALTY_4=0.5760
PRIMER_LEFT_4_PENALTY=0.473868
PRIMER_RIGHT_4_PENALTY=0.102129
PRIMER_LEFT_4_SEQUENCE=ACGTGAAACCGAATCTTCTACC
PRIMER_RIGHT_4_SEQUENCE=CACCGAATTTCATCACACAAGT
PRIMER_LEFT_4=216,22
PRIMER_RIGHT_4=422,22
PRIMER_LEFT_4_TM=59.526
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_01683 Ps_contig_mira-and-tgicl-ass_franssen_15779
SEQUENCE=GGGGGGCTTTACATTATATAAGAAGACGTTTTAGACGAGTGCCGCCGCGTCTCTCTCAGCATTCATTTCACTCACTGCTCTCTCTCTCACTCTCTCACATTTCCATTTCAACTTCTTCTGAAGCTCCTTCTTTCCAGGTCGAGCTTCGGAAAACAAAAATAAATTATTAATTTAGATTCTAGTTTTTCTATTTTCTTCATTCATGGCAACAAGTTCGTTTACATCTTCAATACTACGGAGTGAACGTAGAAACCGAAATACTTA
TARGET=78,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3418
PRIMER_LEFT_PENALTY=0.302618
PRIMER_RIGHT_PENALTY=0.039178
PRIMER_LEFT_SEQUENCE=GAAGACGTTTTAGACGAGTGCC
PRIMER_RIGHT_SEQUENCE=GTAAACGAACTTGTTGCCATGA
PRIMER_LEFT=21,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=60.303
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=201
PRIMER_PAIR_PENALTY_1=0.6880
PRIMER_LEFT_1_PENALTY=0.302618
PRIMER_RIGHT_1_PENALTY=0.385416
PRIMER_LEFT_1_SEQUENCE=GAAGACGTTTTAGACGAGTGCC
PRIMER_RIGHT_1_SEQUENCE=GAACTTGTTGCCATGAATGAAG
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=215,22
PRIMER_LEFT_1_TM=60.303
PRIMER_RIGHT_1_TM=59.615
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=0.8164
PRIMER_LEFT_2_PENALTY=0.302618
PRIMER_RIGHT_2_PENALTY=0.513831
PRIMER_LEFT_2_SEQUENCE=GAAGACGTTTTAGACGAGTGCC
PRIMER_RIGHT_2_SEQUENCE=TTTCGGTTTCTACGTTCACTCC
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=60.303
PRIMER_RIGHT_2_TM=60.514
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=0.9436
PRIMER_LEFT_3_PENALTY=0.904447
PRIMER_RIGHT_3_PENALTY=0.039178
PRIMER_LEFT_3_SEQUENCE=GCGTCTCTCTCAGCATTCATTT
PRIMER_RIGHT_3_SEQUENCE=GTAAACGAACTTGTTGCCATGA
PRIMER_LEFT_3=46,22
PRIMER_RIGHT_3=221,22
PRIMER_LEFT_3_TM=60.904
PRIMER_RIGHT_3_TM=60.039
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=0.9591
PRIMER_LEFT_4_PENALTY=0.919960
PRIMER_RIGHT_4_PENALTY=0.039178
PRIMER_LEFT_4_SEQUENCE=CGTCTCTCTCAGCATTCATTTC
PRIMER_RIGHT_4_SEQUENCE=GTAAACGAACTTGTTGCCATGA
PRIMER_LEFT_4=47,22
PRIMER_RIGHT_4=221,22
PRIMER_LEFT_4_TM=59.080
PRIMER_RIGHT_4_TM=60.039
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_01684 Ps_contig_mira-and-tgicl-ass_franssen_15786
SEQUENCE=GGGGGKCTCAAGAACGAAACTGAAACAAACAAAGGTGAAGARAAAAAAAGGCTCAAAAAATCACTTCAATCTTCGAAATCAACATCCATCATTCAATTCAATTCATCTGGCGCACGCGGAATCAAAAAAAAGATGAAAACGAATTTGAAGCAAGAACTCAATGAATCAGAGTTTCGACATGAACAGGGCAATTGAAACTGTCTTTTTAGATTTGATGTGAATTGGAATTTGGCTTGTAGTAAAA
TARGET=91,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01685 Ps_contig_mira-and-tgicl-ass_franssen_15787
SEQUENCE=TAAGAGAAAAAAAGGAACTCAAAAATACACTTCAATCTTCGAAATACAACATCCATCATTCAATTCAATTCATCTGGCGCACGCGGAATCAAAAAAAGAATGAAAACGAATTTGAAGCAAGAACTCAATGAATCAGAGTTTCGACATGAACAGGGGCAATTGAAACTGGCTTTTTTAGAATTTGATGTGAATTGGAATTTGGCTTGTGAAACTAATTGGCTGCACTTGGCAATTATTTTTGCACGTGAATGAGTTTGGAAGGTTTTTGGAATATTGTTGATCATAAGCTATGATTGGAATGGATTTGGATTATTATATTGACTTGATTGTGTTGTAATTTTGAAGTTGAAATATGTGAAGTGTATTGAATACTGTAACTTGTAATCAATGGTTGAATGTTGGTTTGTGGTTGTAATGTACATGGAATTGAATGAAGATGAATGTATTTGGGTATTGAACACGTATGGCTTATGTATTTGAGAATGGAAATTGAATGGTTGAATGAAATATTGGAATCAATAGAACTTATGTAT
TARGET=58,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01686 Ps_contig_mira-and-tgicl-ass_franssen_15800
SEQUENCE=TTAAGAACGTAGCTCTAGCAAATTAATNATCCATAAGACAGACGCTTCCATTACATCAAGTAGTGTAATTAATACAGTTCAATTTTCGATCGCCAATGWTGATATGAAGGTCAAGTGAAGTGATTGCAGGCTGATATCAAAAGGAATGTGCCCATATGACTTTTCCGGTTGAAAAAGCTCAACACTAGAGACTTGTTGTTTTGGATTTTCTATACAACGCCCAAAGGAATCCTCAAATTTTAACTATCAAACATGTTTTATTAAAAAACCAATAGATGCTGTTACGATTCCAAGTAAACCACCAGCAATTACCTGAGGCGGGGTATGCCCAAGAAGTTCGCGAAGAGGTCTGCTGTCAGACAGAGGATGTTCAGCAGGAAGTTCAATTACAATTTGATTAAGAACCTCCGCTTGTCGTCCTGCTTGCAATCTTACACCAGTAGCATCATACATCACAATGATAGCCAGAACCAATGCAGTAGCGAAAAGTGGTCCTCCAAATCCTTCCCGAAATCCAACCGCTGTAGCAAGAGCAGTAACAGTAGCTGAATGAGAAGACGGCATTCCGCCAGATCCAATCAATTGTTTCGGATCCCATATCCTTTCCTTATACCAAGCGGTGAAGAATTTGATGAGTTGAGCGATGGTAAAAGCAACTATGGCGCAAAAAAGAGGGTAATTGCCAAAGGTTGAAGAAGAAGAAGACGTGGTTGTACCTGCAGCGTCGTCCATGGGTTCTCTATTAATTAATTAAATTAATTAATTAACCAGTAAATGAATTAATTAATTAATNAATTAATAGATGAGAATGAATTGGGAAATTNTGGGTAGATTTTAAGAAATGCAGTGCA
TARGET=692,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01687 Ps_contig_mira-and-tgicl-ass_franssen_15800
SEQUENCE=TTAAGAACGTAGCTCTAGCAAATTAATNATCCATAAGACAGACGCTTCCATTACATCAAGTAGTGTAATTAATACAGTTCAATTTTCGATCGCCAATGWTGATATGAAGGTCAAGTGAAGTGATTGCAGGCTGATATCAAAAGGAATGTGCCCATATGACTTTTCCGGTTGAAAAAGCTCAACACTAGAGACTTGTTGTTTTGGATTTTCTATACAACGCCCAAAGGAATCCTCAAATTTTAACTATCAAACATGTTTTATTAAAAAACCAATAGATGCTGTTACGATTCCAAGTAAACCACCAGCAATTACCTGAGGCGGGGTATGCCCAAGAAGTTCGCGAAGAGGTCTGCTGTCAGACAGAGGATGTTCAGCAGGAAGTTCAATTACAATTTGATTAAGAACCTCCGCTTGTCGTCCTGCTTGCAATCTTACACCAGTAGCATCATACATCACAATGATAGCCAGAACCAATGCAGTAGCGAAAAGTGGTCCTCCAAATCCTTCCCGAAATCCAACCGCTGTAGCAAGAGCAGTAACAGTAGCTGAATGAGAAGACGGCATTCCGCCAGATCCAATCAATTGTTTCGGATCCCATATCCTTTCCTTATACCAAGCGGTGAAGAATTTGATGAGTTGAGCGATGGTAAAAGCAACTATGGCGCAAAAAAGAGGGTAATTGCCAAAGGTTGAAGAAGAAGAAGACGTGGTTGTACCTGCAGCGTCGTCCATGGGTTCTCTATTAATTAATTAAATTAATTAATTAACCAGTAAATGAATTAATTAATTAATNAATTAATAGATGAGAATGAATTGGGAAATTNTGGGTAGATTTTAAGAAATGCAGTGCA
TARGET=742,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01688 Ps_contig_mira-and-tgicl-ass_franssen_15800
SEQUENCE=TTAAGAACGTAGCTCTAGCAAATTAATNATCCATAAGACAGACGCTTCCATTACATCAAGTAGTGTAATTAATACAGTTCAATTTTCGATCGCCAATGWTGATATGAAGGTCAAGTGAAGTGATTGCAGGCTGATATCAAAAGGAATGTGCCCATATGACTTTTCCGGTTGAAAAAGCTCAACACTAGAGACTTGTTGTTTTGGATTTTCTATACAACGCCCAAAGGAATCCTCAAATTTTAACTATCAAACATGTTTTATTAAAAAACCAATAGATGCTGTTACGATTCCAAGTAAACCACCAGCAATTACCTGAGGCGGGGTATGCCCAAGAAGTTCGCGAAGAGGTCTGCTGTCAGACAGAGGATGTTCAGCAGGAAGTTCAATTACAATTTGATTAAGAACCTCCGCTTGTCGTCCTGCTTGCAATCTTACACCAGTAGCATCATACATCACAATGATAGCCAGAACCAATGCAGTAGCGAAAAGTGGTCCTCCAAATCCTTCCCGAAATCCAACCGCTGTAGCAAGAGCAGTAACAGTAGCTGAATGAGAAGACGGCATTCCGCCAGATCCAATCAATTGTTTCGGATCCCATATCCTTTCCTTATACCAAGCGGTGAAGAATTTGATGAGTTGAGCGATGGTAAAAGCAACTATGGCGCAAAAAAGAGGGTAATTGCCAAAGGTTGAAGAAGAAGAAGACGTGGTTGTACCTGCAGCGTCGTCCATGGGTTCTCTATTAATTAATTAAATTAATTAATTAACCAGTAAATGAATTAATTAATTAATNAATTAATAGATGAGAATGAATTGGGAAATTNTGGGTAGATTTTAAGAAATGCAGTGCA
TARGET=754,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01689 Ps_contig_mira-and-tgicl-ass_franssen_15800
SEQUENCE=TTAAGAACGTAGCTCTAGCAAATTAATNATCCATAAGACAGACGCTTCCATTACATCAAGTAGTGTAATTAATACAGTTCAATTTTCGATCGCCAATGWTGATATGAAGGTCAAGTGAAGTGATTGCAGGCTGATATCAAAAGGAATGTGCCCATATGACTTTTCCGGTTGAAAAAGCTCAACACTAGAGACTTGTTGTTTTGGATTTTCTATACAACGCCCAAAGGAATCCTCAAATTTTAACTATCAAACATGTTTTATTAAAAAACCAATAGATGCTGTTACGATTCCAAGTAAACCACCAGCAATTACCTGAGGCGGGGTATGCCCAAGAAGTTCGCGAAGAGGTCTGCTGTCAGACAGAGGATGTTCAGCAGGAAGTTCAATTACAATTTGATTAAGAACCTCCGCTTGTCGTCCTGCTTGCAATCTTACACCAGTAGCATCATACATCACAATGATAGCCAGAACCAATGCAGTAGCGAAAAGTGGTCCTCCAAATCCTTCCCGAAATCCAACCGCTGTAGCAAGAGCAGTAACAGTAGCTGAATGAGAAGACGGCATTCCGCCAGATCCAATCAATTGTTTCGGATCCCATATCCTTTCCTTATACCAAGCGGTGAAGAATTTGATGAGTTGAGCGATGGTAAAAGCAACTATGGCGCAAAAAAGAGGGTAATTGCCAAAGGTTGAAGAAGAAGAAGACGTGGTTGTACCTGCAGCGTCGTCCATGGGTTCTCTATTAATTAATTAAATTAATTAATTAACCAGTAAATGAATTAATTAATTAATNAATTAATAGATGAGAATGAATTGGGAAATTNTGGGTAGATTTTAAGAAATGCAGTGCA
TARGET=778,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01690 Ps_contig_mira-and-tgicl-ass_franssen_15801
SEQUENCE=ATNAGTGAGNGATGTAAAGNAACATGGCGCAAAAANAGGGTAATTGCCAAAGGTTGAAGAAGAAGAAGACGTGGTTGTACCTGCAGCGTCGTCCATGGGGTTCTCTATTAATTAATTAATTAATTAATTAACCAGTAAATGAATTAATTAATTAATCAATTAATAGATGAGGATGAATTGGGGAATTCTGGGTAGATTTTAAGAATGCAAG
TARGET=56,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1637
PRIMER_LEFT_PENALTY=1.081205
PRIMER_RIGHT_PENALTY=0.082508
PRIMER_LEFT_SEQUENCE=ANAGGGTAATTGCCAAAGGTTG
PRIMER_RIGHT_SEQUENCE=AATCTACCCAGAATTCCCCAAT
PRIMER_LEFT=34,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=61.081
PRIMER_RIGHT_TM=59.917
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=1.4237
PRIMER_LEFT_1_PENALTY=1.341209
PRIMER_RIGHT_1_PENALTY=0.082508
PRIMER_LEFT_1_SEQUENCE=NAGGGTAATTGCCAAAGGTTG
PRIMER_RIGHT_1_SEQUENCE=AATCTACCCAGAATTCCCCAAT
PRIMER_LEFT_1=35,21
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=60.341
PRIMER_RIGHT_1_TM=59.917
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=1.4538
PRIMER_LEFT_2_PENALTY=1.081205
PRIMER_RIGHT_2_PENALTY=0.372603
PRIMER_LEFT_2_SEQUENCE=ANAGGGTAATTGCCAAAGGTTG
PRIMER_RIGHT_2_SEQUENCE=TCCCCAATTCATCCTCATCTAT
PRIMER_LEFT_2=34,22
PRIMER_RIGHT_2=183,22
PRIMER_LEFT_2_TM=61.081
PRIMER_RIGHT_2_TM=59.627
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=1.7138
PRIMER_LEFT_3_PENALTY=1.341209
PRIMER_RIGHT_3_PENALTY=0.372603
PRIMER_LEFT_3_SEQUENCE=NAGGGTAATTGCCAAAGGTTG
PRIMER_RIGHT_3_SEQUENCE=TCCCCAATTCATCCTCATCTAT
PRIMER_LEFT_3=35,21
PRIMER_RIGHT_3=183,22
PRIMER_LEFT_3_TM=60.341
PRIMER_RIGHT_3_TM=59.627
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=1.7147
PRIMER_LEFT_4_PENALTY=1.081205
PRIMER_RIGHT_4_PENALTY=0.633469
PRIMER_LEFT_4_SEQUENCE=ANAGGGTAATTGCCAAAGGTTG
PRIMER_RIGHT_4_SEQUENCE=TTCCCCAATTCATCCTCATCTA
PRIMER_LEFT_4=34,22
PRIMER_RIGHT_4=184,22
PRIMER_LEFT_4_TM=61.081
PRIMER_RIGHT_4_TM=60.633
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_01691 Ps_contig_mira-and-tgicl-ass_franssen_15801
SEQUENCE=ATNAGTGAGNGATGTAAAGNAACATGGCGCAAAAANAGGGTAATTGCCAAAGGTTGAAGAAGAAGAAGACGTGGTTGTACCTGCAGCGTCGTCCATGGGGTTCTCTATTAATTAATTAATTAATTAATTAACCAGTAAATGAATTAATTAATTAATCAATTAATAGATGAGGATGAATTGGGGAATTCTGGGTAGATTTTAAGAATGCAAG
TARGET=107,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2111
PRIMER_LEFT_PENALTY=0.128591
PRIMER_RIGHT_PENALTY=0.082508
PRIMER_LEFT_SEQUENCE=GCCAAAGGTTGAAGAAGAAGAA
PRIMER_RIGHT_SEQUENCE=AATCTACCCAGAATTCCCCAAT
PRIMER_LEFT=45,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.871
PRIMER_RIGHT_TM=59.917
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.4265
PRIMER_LEFT_1_PENALTY=0.343977
PRIMER_RIGHT_1_PENALTY=0.082508
PRIMER_LEFT_1_SEQUENCE=GGTAATTGCCAAAGGTTGAAGA
PRIMER_RIGHT_1_SEQUENCE=AATCTACCCAGAATTCCCCAAT
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=60.344
PRIMER_RIGHT_1_TM=59.917
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.6709
PRIMER_LEFT_2_PENALTY=0.588441
PRIMER_RIGHT_2_PENALTY=0.082508
PRIMER_LEFT_2_SEQUENCE=AAGAAGACGTGGTTGTACCTGC
PRIMER_RIGHT_2_SEQUENCE=AATCTACCCAGAATTCCCCAAT
PRIMER_LEFT_2=62,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=60.588
PRIMER_RIGHT_2_TM=59.917
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.7166
PRIMER_LEFT_3_PENALTY=0.343977
PRIMER_RIGHT_3_PENALTY=0.372603
PRIMER_LEFT_3_SEQUENCE=GGTAATTGCCAAAGGTTGAAGA
PRIMER_RIGHT_3_SEQUENCE=TCCCCAATTCATCCTCATCTAT
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=183,22
PRIMER_LEFT_3_TM=60.344
PRIMER_RIGHT_3_TM=59.627
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.8237
PRIMER_LEFT_4_PENALTY=0.741209
PRIMER_RIGHT_4_PENALTY=0.082508
PRIMER_LEFT_4_SEQUENCE=TTGCCAAAGGTTGAAGAAGAAG
PRIMER_RIGHT_4_SEQUENCE=AATCTACCCAGAATTCCCCAAT
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=197,22
PRIMER_LEFT_4_TM=60.741
PRIMER_RIGHT_4_TM=59.917
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_01692 Ps_contig_mira-and-tgicl-ass_franssen_15801
SEQUENCE=ATNAGTGAGNGATGTAAAGNAACATGGCGCAAAAANAGGGTAATTGCCAAAGGTTGAAGAAGAAGAAGACGTGGTTGTACCTGCAGCGTCGTCCATGGGGTTCTCTATTAATTAATTAATTAATTAATTAACCAGTAAATGAATTAATTAATTAATCAATTAATAGATGAGGATGAATTGGGGAATTCTGGGTAGATTTTAAGAATGCAAG
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2111
PRIMER_LEFT_PENALTY=0.128591
PRIMER_RIGHT_PENALTY=0.082508
PRIMER_LEFT_SEQUENCE=GCCAAAGGTTGAAGAAGAAGAA
PRIMER_RIGHT_SEQUENCE=AATCTACCCAGAATTCCCCAAT
PRIMER_LEFT=45,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.871
PRIMER_RIGHT_TM=59.917
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.4265
PRIMER_LEFT_1_PENALTY=0.343977
PRIMER_RIGHT_1_PENALTY=0.082508
PRIMER_LEFT_1_SEQUENCE=GGTAATTGCCAAAGGTTGAAGA
PRIMER_RIGHT_1_SEQUENCE=AATCTACCCAGAATTCCCCAAT
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=60.344
PRIMER_RIGHT_1_TM=59.917
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.6709
PRIMER_LEFT_2_PENALTY=0.588441
PRIMER_RIGHT_2_PENALTY=0.082508
PRIMER_LEFT_2_SEQUENCE=AAGAAGACGTGGTTGTACCTGC
PRIMER_RIGHT_2_SEQUENCE=AATCTACCCAGAATTCCCCAAT
PRIMER_LEFT_2=62,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=60.588
PRIMER_RIGHT_2_TM=59.917
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.7166
PRIMER_LEFT_3_PENALTY=0.343977
PRIMER_RIGHT_3_PENALTY=0.372603
PRIMER_LEFT_3_SEQUENCE=GGTAATTGCCAAAGGTTGAAGA
PRIMER_RIGHT_3_SEQUENCE=TCCCCAATTCATCCTCATCTAT
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=183,22
PRIMER_LEFT_3_TM=60.344
PRIMER_RIGHT_3_TM=59.627
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.7655
PRIMER_LEFT_4_PENALTY=0.682954
PRIMER_RIGHT_4_PENALTY=0.082508
PRIMER_LEFT_4_SEQUENCE=TCGTCCATGGGGTTCTCTATTA
PRIMER_RIGHT_4_SEQUENCE=AATCTACCCAGAATTCCCCAAT
PRIMER_LEFT_4=88,22
PRIMER_RIGHT_4=197,22
PRIMER_LEFT_4_TM=60.683
PRIMER_RIGHT_4_TM=59.917
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_01693 Ps_contig_mira-and-tgicl-ass_franssen_15829
SEQUENCE=CAATTCTATGTATACCTCTTCATCTCAMCGACATGGTAAATAAATAAATGGCCATTGGCCACCCACTATCAYAGTTTTTCTATCCATAAGTAGAATGTATTTACCTCTTTGTTTTAAGGGCTTTTCTTATCTTAAGGTTGGAATATCATCCCTTCGTAGTGAGTCACACAGCCTTTAAGGACACGATAAAATGACTAACATGCTTGTCACCATGGCAAGATATGCGGGCCCAATGAATTAAACAATGATATAGAGGTTCCGACAATTCTTTATGCATAGGAAACGTGGGTTCATGAACCTAATTTTGTAGGGAAACGTGGCTCATGAACCTAATCTTAAGG
TARGET=37,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01694 Ps_contig_mira-and-tgicl-ass_franssen_15832
SEQUENCE=TCCAGAGTGTYGCGCYTTRCAGCATCGACTGAAAAATTCTTATAAGCGATAGACATCRATAATGAGTTATTRTTWTGTAATTTTTCAGGAGATGTGCAAGTAGTATTTTGGTTTAGTACTACTACTACTACCTAGTTGATGGCCTCTAAGCTCTCTTAAATGCATTTGGCACTTTGCTTTGAGCCATCGTAGTTCATGCCTTATATCATTTTCATAGTCCAAGAATAATGAACATTCTGA
TARGET=117,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01695 Ps_contig_mira-and-tgicl-ass_franssen_15833
SEQUENCE=AATTGAAAGACATTCATTGTGATAAATCTCATGAGATATCTAAACTATCACTCATAAAGGAGGATGAAGAGGAGAATAGCGACCAAATTTATCTTAATACCAAAAAGCCTCCTTCTAGCCCTGCAGCATCAGAATGTTCATTATTCTTGGACTATGAAAATGATATAAGGCATGAACTACGATGGCTCAAAGCAAAGTACCAAATGCATTTAAGAGAKCTTAGAGGCCATCAACTAGGTAGTAGTAGTAGTAAACCAAAATCTACTTGCACATCTCCTGAAAAATTACATAACAATA
TARGET=238,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01696 Ps_contig_mira-and-tgicl-ass_franssen_15852
SEQUENCE=GATTACATAGATAAACTAAWMRWYAWTGCACTTTTTCCTTTTTCCTTTTTTTATTGAACAGACACTTTTCTGATCTTACAGAGAAAAAAAAGAAACTTATTTTCACCCCCTTATTTAGACATTATGGAGCCTCAAAACGCAAAGTTCATCATGTCCATGCTCAAGCTTCGCATCATAACATTTTCCATGATGAATCCACACTTGGTGGGAGCCTTCTTAATGCTCTGGCTTCTTGCCTTGAAAGAGCATGAAGAAGAAGAAATAGGCACTATTGGGATATTCTCACAGTTGCAAAGTTCTATCATGTATCCATCTGGGTCATGGAAGAACACCTGCTCCACCTTGATTCCTTCATCCTCTACCAATGCTGTAACATACTTCATCCCCATTTCTTCCAACCTCATTTTCACAAGTCCAACATCTGTACACTGGAATGATATGTGGTTGTCCTTGGGATTAATGGGTCGTGGCTCATCCATAGGAGTATCATCAAATGCATCATAGTTTGGATTCTCAAGCAGGTGTATTCCAAAACCATAATTATACAACCAGGCTCCATTGAATTTGAAAGAGGAAGGGCGTTTAATGAGACCAAAACCCAAGACTTCTTCATAAAATCTCATAGAGTCTAACACTGATCTGCACAATAGAGAGACATGATTCAGGGAGAGAAGTGGTAGTGGTGCTTCGTAGCTGCCAGTTATCTCCATGTTCATATTTTCTCTCTTTCAACACTTCACTCACCTAAACTTTTTTAGATGTTAGCTTTGAAGTGTSWMYYCGA
TARGET=250,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01697 Ps_contig_mira-and-tgicl-ass_franssen_15860
SEQUENCE=CGTCTCGTCATCCTCTGTTTTACGATCCCGGTTCTTTTTTCTTGTTCAATCCGTTTGCAGCTTTCTTGACTTTCTTGTGATGTCTACGGAGTTTCTTCCATTTTCCGAGGATCGCGCGGCTCCAGCGTGTTCTTCCTTTGGCTGATGTGGCGAGGACTCGATCGGCGGTTTCGCGGACTTGACCGGCGGCTCTAGGTTTGGTGGTTGCCGGGGAATTGATCCGGCTGAGAGCTTCGATTAGTTTTTTGGAGTAGTTGTTCTGGTCTGATTCAGATCTCCATCGCATAAGAGTGAGGGAATTTTGATCGGCGGTTATATCTCCGATTTTGCGGCGTTTCTTGTGATTGGATTCTTGCAAAGTGTGTGATGGTTCGAATGAATCCATTGATGAATTGGAATCGAATGGTTGAGTTGTTTGAAGATTGAGTTAAGGAATTGATTGAAGAGTTAGAATCGAGAATTGGAGAAGAAGAAGATGAAGAGGATGGTTTGTTGTATGGTAGGAATTGTGAGGAGCAGGGAAGAGAAGAGAGAAAAGAGTATATAACAGCAGTTTAGGGTCACCGGGGCCCCGTGTACTCTGG
TARGET=465,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6708
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.633469
PRIMER_LEFT_SEQUENCE=ATTCTTGCAAAGTGTGTGATGG
PRIMER_RIGHT_SEQUENCE=CTCTCTTCTCTTCCCTGCTCCT
PRIMER_LEFT=348,22
PRIMER_RIGHT=532,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.633
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=0.6913
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.653952
PRIMER_LEFT_1_SEQUENCE=ATTCTTGCAAAGTGTGTGATGG
PRIMER_RIGHT_1_SEQUENCE=TTCTCTCTTCTCTTCCCTGCTC
PRIMER_LEFT_1=348,22
PRIMER_RIGHT_1=534,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.346
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.7289
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.633469
PRIMER_LEFT_2_SEQUENCE=TCGCATAAGAGTGAGGGAATTT
PRIMER_RIGHT_2_SEQUENCE=CTCTCTTCTCTTCCCTGCTCCT
PRIMER_LEFT_2=280,22
PRIMER_RIGHT_2=532,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.633
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.7356
PRIMER_LEFT_3_PENALTY=0.102129
PRIMER_RIGHT_3_PENALTY=0.633469
PRIMER_LEFT_3_SEQUENCE=AAAGTGTGTGATGGTTCGAATG
PRIMER_RIGHT_3_SEQUENCE=CTCTCTTCTCTTCCCTGCTCCT
PRIMER_LEFT_3=356,22
PRIMER_RIGHT_3=532,22
PRIMER_LEFT_3_TM=59.898
PRIMER_RIGHT_3_TM=60.633
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=0.7494
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=0.653952
PRIMER_LEFT_4_SEQUENCE=TCGCATAAGAGTGAGGGAATTT
PRIMER_RIGHT_4_SEQUENCE=TTCTCTCTTCTCTTCCCTGCTC
PRIMER_LEFT_4=280,22
PRIMER_RIGHT_4=534,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=59.346
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_01698 Ps_contig_mira-and-tgicl-ass_franssen_15871
SEQUENCE=CGTGTCTCAGTGTTCAAGRACTTGTGTAGAAGTAATTCTCTTCTTTTGGATCCGGATTCAGCRTCTTCCTGACTAGATCTTTAGCACTGTCTGATATTGATGGCCATGGTTCACTAACAAAATCAAGTTCGCCTTCCAATATGGCATTAAATATTCCCTTTTCAGTTTCAGCCCAAAAAGGTGGTACACCACTAAGGAGAATATACAATATAATGCCTGCACTCCAAATGTCTATTTCCTTCCCATAACTACGACGCAGTACCTCAGGAGCAACATAGTAAGCACTGCCTACCATATCACGGTAAACCTTGCCTTCTTCGATGAAAACAGAAAGTCCAAAATCAGTAGCCTTGAGCGCCGAACCTTCGTCCTTACTAGACAGCAAGAAATTCTCCGGCTTCAAATCGCGATGCAAAACACCCATAAAATGACAAATATGCACAACATTAACAATAGCCCTACAAAGAGATGCAGCAGCTCTCTCTGAATAATGACCTTGCGCAATAATCCTATCAAACAACTCTCCACCAGCACAAAGCTCCATCACAAGATGCACAGAAAATCTATCCTCATAAGCACCTTTAAATTCAACAATATTAGGTTGACCTGATAAATGCTGCAAAATCTGAATCTCTCTCTTAATATCTTCTCTATCAGCTTTGCTCACAAGCTTCCTTTTCAATATAGATTTACAAGCATAATTCAAACCAGTAGAACTCTCAGTGCAGAAATACGTAATCCCAAATTGACCTCGCCCTAATTCTTTACCTAAACTATAGAACTTCTTAATATCTTACAAAAGGTTTACCTAATATAGCTGTATCGGTTTTCTGCACAGTTTTCACATTCTGAGGTGTGACAGAGGGTTTAGGATTCGGTGTTGTTGTTATCACTGTTGAAGAAGCTTGTGAGTGTGAATTAGGTTTTGGTGGTGATGAAGTCTGAACATGACTGGAAGATGATTGAACACTGTGGTAGTGAGTATTACTACTACCACCTCCACCACCGCCGCCACCACCTCCGCCGGTGACGGATGGTCCACGGCGGGTACTAGTAACTTCCTTTGGTTTCCTCTCCTTGCTACCGCGACAACCCATTATCGAAGGAGGCTATGATTAGAGAGAGAGAGAGAGATGATGATGAGTATAACGCGGASTAAGGGASTATTAAGAAATAAGAAAAAGCAGTATTAAGATAAGGAAAAAAGGAGGGTTAA
TARGET=1118,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01699 Ps_contig_mira-and-tgicl-ass_franssen_15872
SEQUENCE=GTCGGGGKACACCAATTCATTCACACACTCTTTGTTCTAAACCACACAAGAACAAACATAGTAACATAAACACATAAAAACAAACAACAGTTTCTTCAATGTCTTCTATTGTTACTTCAAGTGTTATCAACGTTCCACGTTCTTCTTCTTCATCAAAGAACCTCTCATTCTCATCATCACAACTCTCCGGTGACAAGATTCTTACAGTTTCAGGTAAGGGTGCACCAAGAGGAAGATGCACCCGCAAGCATGTGATTGTTACTCCTAAAGCTGTTTCTGATTCACAGAACTCTCAAACTTGCCTTGATCCTGATGCTAGCAGAAGTGTGCTTGGAATTATACTTGGAGGTGGTGCTGGTACTCGTCTTTATCCACTCACCAAGAAGAGAGCAAAACCTGCTGTTCCTCTTGGAGCTAACTATAGACTCATTGATATTCCTGTTAGCAATTGCTTGAATAGCAACATTTCTAAGATCTATGTTCTTACTCAATTCAATTCCGCCTCACTCAATCGACATCTTTCTCGCGCTTATGCGAGTAATTTGGGTGGTTACAAAAATGAGGGTTTTGTTGAAGTTCTTGCTGCTCAGCAAAGTCCTGAGAATCCTAATTGGTTTCAGGGTACTGCAGATGCTGTGAGGCAATATTTATGGCTTTTTGAAGAGCATAATGTTTTGGAGTACTTAATTCTGGCGGGTGACCATTTGTATCGAATGGATTATGAGAAATTTATCCAAGCACATAGGGAATCTGATGCTGATATCACCGTGGCTGCGTTGCCAATGGATGAAAAGCGTGCAACTGCTTTCGGTTTGATGAAGATCGATGAAGAGGGGCGTATAATTGAGTTTGCAGAAAAGCCGAAAGGAGAACAGTTGAAAGCTATGAAGGTTGATACTACGATTTTAGGTCTTGACGATGAAAGAGCGAAAGAAATGCCTTTTATTGCTAGCATGGGTATATATGTTATCAGCAAAAATGTGATGCTAGACCTTCTCCGCGACAAGTTTCCCGGTGCAAATGACTTTGGGAGTGAAGTGATTCCTGGTGCTACTTCTGTTGGAATGAGAGTGCAAGCTTACTTATATGATGGCTACTGGGAAGACATTGGTACCATTGAGGCTTTCTATAATGCAAATCTTGGAATCACCAAAAAGCCTGTGCCTGATTTCAGTTTCTATGATCGTTCATCTCCGATTTACACCCAACCGCGATACTTGCCTCCATCTAAGATGCTTGATGCTGATATTACTGATAGTGTTATCGGAGAAGGATGTGTGATTAAGAACTGCAAGATTTTCCACTCTGTGGTCGGGCTGCGATCTTGCATATCAGAAGGTGCAATTATTGAAGACACTTTGTTAATGGGGGCAGATTATTACGAGACAGAAGCTGATAAAAGGTTTTTGGCTGCTAAAGGCAGTGTTCCAATTGGTATCGGCAAAAAYTCGGSATATCAAAAGAGCAATTGTTGACAAGAACGCGAGAATCGGAGAAAACGTCAAGATAATTAACAGTGACAATGTTCAAGAAGCTGCTAGGGAAACAGAAGGCTATTTCATCAAAAGCGGGATCGTCACAATAATCAAGGATGCCTTGATTCCTAGTGGAACTGTCATATAGAAGACTGAAACTCATCTTTTGTTTCATAATGGTGAGTGAGACTTTGAAGTTCATGTTGCATGATATATTTATTTTTCGATAAAGGCTTGTAAATAATAGCTGAAGAGAGAACCTTCTTGTGTTTTGGAAACTAGTACTGGTAAAGTTTGTTGATCAATTGAATAAAAGTCGTTTTATTTTACCTAAAAGT
TARGET=140,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01700 Ps_contig_mira-and-tgicl-ass_franssen_15878
SEQUENCE=ATTTACCCAAAAAAAACCAACAAAAAATACCAAATCAAATCAAATCATGAACACCATGAAACTCAGACTCAGATCTTTCGAATCCAAAGAAACCCTAAAATTCGAAGTTCCCGATCCATGTTCTCTTCAACAACTCAAATACACCATTTCTACCTAATCCCTTCTTCTTCTTCTTCCTC
TARGET=31,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01701 Ps_contig_mira-and-tgicl-ass_franssen_15878
SEQUENCE=ATTTACCCAAAAAAAACCAACAAAAAATACCAAATCAAATCAAATCATGAACACCATGAAACTCAGACTCAGATCTTTCGAATCCAAAGAAACCCTAAAATTCGAAGTTCCCGATCCATGTTCTCTTCAACAACTCAAATACACCATTTCTACCTAATCCCTTCTTCTTCTTCTTCCTC
TARGET=161,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01702 Ps_contig_mira-and-tgicl-ass_franssen_15879
SEQUENCE=TTTTTTTTAAAAAAGACTCAGTGACAATGAAATAAATCTAAACAGCAAAAACAATGCGGCTCCATTTGGTAAAAATTATTCTGAAACATCATCATCTACATGCTGACGCTGAACAGAAATTGGTTTTTACCCAAACATTGGAACCAAWGACATWGAGAWACAATTATWTTCTTCCTAATATCAGACATACATGGATTCTATTCAAAATGTATAACCAAAAGGAATAACATTTTATAAATCTTATTACATCATTCACAACCAGTTAAGTCCAAGCACGGATTATTCATCAAAATTCTCCCAAATGACATGGGGGAAAGAAAGTTCGCCTGGGTGTGTATGCAGGGGGGAGATTTAAACCAAAATTTGGCTGCAGATCATACTCACCACCCCAAATTGGAGGCATTCCAAAAGGAATAGGACCTCTTCTCCTCTGAAAGTATCTCATAAAACCAGACCGAGGATTTCGAAAAGGTGGCGGTGGTTGTTCTGATTTCTTCTTAGTTGCCCGATAATGTGCAAACAAGTTCTTAAAAAACTTCATACCATTCACCCTATCTCCAAACTCCTCTTCAAACTTTTTCCTCCATAACTCATCATTCGACGCAAGGTACGACAACTCCGAACATGTGCAACACACTTTGGCCAAATCATCACCCGACAGATACTCAAATATCAGAAGCTTAAGTTCCATTGGAAGTCGCATAAAACAAGGTGGAAGATCCAATCCAGCTTTATCACAAAGATCAATCAAAAGTGGCAATGCAAGCTTATCTTTCACTATCTTCCACAATTCAAAAACTTCATCATCACCACCATCATTAACATTAACACTAACACTAGCACTAGGTTCAGAATTTGCCAGCATTAACTCTAAAGGCTTAGCAAATCTAAATTTATCTAAATAAACCCTATGAACCCTAGACCCAACATCATCAAACAACGAACCATAAACATTCACAAAATTCCCTAAAGTTTGAATTTTTAAATCCACAGCATGTGATAAGCCTTTAGAAACAATCTCAGGAAGCGTATACCTCAAAGATATCATTGAAGAAGAAGATACGTCATCATTCGCCTTCCCTGAATCTCGACCAACACGAACAAACCCAGATTCGAGAATAACAGCATGAACTGCGAATACCAAAAGTTTGAAATCGCTAACATCATCGCCAAGCGCTTCTTTAAGAATCGTTTTCACGAAATCAGGTTCCGAATTGTTCTTCCCCGCTAAAACCGCGGGTTCATCAGACACATCCACCATTTCTAGGGTTTGCACTTCCGCGGGCTTTTCTGCGAAAGTCGGAGGGTTTTCTGCGGCAGGTTTGTGAGGGAGGGTTTGACCGGAGAAGGCGTTCGAGTTGAAAGTGTAGAAGATGAGGTCGCCGCCGGCGATGCCGATTGAGTGGAGGGAATCGGTCGGAGAAGAAGTGTTGATTTCGTCTTTTCTGTTGAGGGAAAGATGAAGAGAAGAAGAGGAAGAAGAAGGAGAAGGGATTAGGGTAGAAATGGTGTATTTGAGTTGTTGAAGAGAACATGGATCGGGAACTTCGAATTTTAGGGTTTCTTTGGATTCGAAAGATCTGAGTCTGAGTTTCATGGTGTTCATGATTTGATTTGATTTGGATTTTTGTGTTTTTTTGGGTAAATTCTTTGTCTCAAHDCKTMCYCCCGCCGGTACTTCTTGCGGTTTGAA
TARGET=1606,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01703 Ps_contig_mira-and-tgicl-ass_franssen_15884
SEQUENCE=GAGAGTTTCATGAATATCCAAGCGCTTGTGTCTGTGAGAAACCATTTATAGATAAACTGAGYGAAGATGGAATTCACCCGCCTAAACTCGCGTCGAGAAATATGACAACGGGAACTGACTCTGAAATAGGTAGTGGAAATGAAGAAAATTTTGAGCTACCAAAGCCAAGTGAGAGAACATGACATTATGATGATGATGAGAATTGAAAAAGCAACTTTTAGGTACCTTAAGTTGGTAGTAATATT
TARGET=187,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01704 Ps_contig_mira-and-tgicl-ass_franssen_15896
SEQUENCE=AGGGAGTGAGTGCGATGTGGCGAAGGTGAAAGAGAGAGCAAAGATGGAGAAAATGATTGCAGTGGGAGTGATATGGGGCGCCACCAACGCTATCATGCGCCGTGGTGCACTTCTTTGGGACGAAGCACTCAAATCCTCTTCCGCAAAACAACCACATCCTCAATCAACAAATCTCGCTCAAAAAATCGCTTTCTCTCTACGCAACTGGCTTAAGCTTCTCTCCATTTGGCAATACTCGATCCCCTTTCTCGTTAATCTCTCCGCCTCCGCCACTTTCTTCTCCATTCTCTCCGACGCTCCTCTTTCCCTCGCCGTCCCCGTCACCAATGCCACCACCTTCGCCTCCACCGCTCTCTTTGCTACACTCCTCGGCGAACAAACTAATCTCCCCCGCACTTTCTTCGGTACTGCTCTCATCATCACCGGAATTTGCTTATGTATCAATKCATAGCTTGATTTCTTTCTTTCCGTTTCATTTATTTTCATTTCTATAAAATAGTATAGTAGTAAATTAGGAAGTTAACAAGATTCGAATTCAATTATTAAGATAGGGTTTATGTTTGACATAAGAAAAAAAAACG
TARGET=457,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01705 Ps_contig_mira-and-tgicl-ass_franssen_15897
SEQUENCE=AACAAACTAATCTCCCCCGCCACTTTCTTCGGTACTGCTCTCATCATCACCGGAATTTGCTTATGTATCAATTCATAGCTTGATTTCTTTCTTTCCGTTTCATTATTTTCATTTCTGTAAAATAGTATGTGTAATTGGAGTTACAAGATTCGAATTCAATTATTAAGATAGGGTTTATGTTTGACATGAATTGAGTATAACAACATTGCAATATGCAGCACCGATATCTCTGAAATGTGATCGTGCCACTATCCATCTGAAA
TARGET=84,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2852
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.269968
PRIMER_LEFT_SEQUENCE=TTCTTCGGTACTGCTCTCATCA
PRIMER_RIGHT_SEQUENCE=CATTTCAGAGATATCGGTGCTG
PRIMER_LEFT=24,22
PRIMER_RIGHT=236,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.730
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.3268
PRIMER_LEFT_1_PENALTY=0.056850
PRIMER_RIGHT_1_PENALTY=0.269968
PRIMER_LEFT_1_SEQUENCE=CCACTTTCTTCGGTACTGCTCT
PRIMER_RIGHT_1_SEQUENCE=CATTTCAGAGATATCGGTGCTG
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=236,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=59.730
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=218
PRIMER_PAIR_PENALTY_2=0.4100
PRIMER_LEFT_2_PENALTY=0.015253
PRIMER_RIGHT_2_PENALTY=0.394711
PRIMER_LEFT_2_SEQUENCE=TTCTTCGGTACTGCTCTCATCA
PRIMER_RIGHT_2_SEQUENCE=ATAGTGGCACGATCACATTTCA
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=251,22
PRIMER_LEFT_2_TM=60.015
PRIMER_RIGHT_2_TM=60.395
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=228
PRIMER_PAIR_PENALTY_3=0.4185
PRIMER_LEFT_3_PENALTY=0.015253
PRIMER_RIGHT_3_PENALTY=0.403233
PRIMER_LEFT_3_SEQUENCE=TTCTTCGGTACTGCTCTCATCA
PRIMER_RIGHT_3_SEQUENCE=GAGATATCGGTGCTGCATATTG
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=229,22
PRIMER_LEFT_3_TM=60.015
PRIMER_RIGHT_3_TM=59.597
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.4516
PRIMER_LEFT_4_PENALTY=0.056850
PRIMER_RIGHT_4_PENALTY=0.394711
PRIMER_LEFT_4_SEQUENCE=CCACTTTCTTCGGTACTGCTCT
PRIMER_RIGHT_4_SEQUENCE=ATAGTGGCACGATCACATTTCA
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=251,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=60.395
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_01706 Ps_contig_mira-and-tgicl-ass_franssen_15902
SEQUENCE=CACAGGCTGTGGCTTCACCTAAACAAAGTCAACATCTATGGCATCATCTAGCAATGTTTTTCCGAGATGAGCTACTGTCTTGGTCTTGGAGAAGACCACTTGGGATGCCCATGGCCCCTATGGCTGCAGGCGGTACCATGAGTCCTGTTGACTTTAAACAAAAGGTTATGACAAATGTGGAACATGTCGTTGCAAGGGTTAAGGGAATAGCACCGCAGAATTTTTCTGACGAGGAGGAGAATGTCATGGAGCCACCCCAATCTGTACAGCGGGGTGTTACTGAGTTGGTTGAAGCTGCCTTAAACCCAAGGAACTTGTGCATGATGGATCCGACATGGCATCCCTGGTTCTAAGATATATCTAGCAGTACTGTCTGTGTACATATGTGTACATATTCCAGCTAATGTGAGCAATACTAGTATTAATTGAAACCCAAGGGTGAATCTGAAACTTGGGTCCCTATTTTTAAGGGTTAGAATCCGCTGTAGAACATTAATTACAGGAATATGACTGAGTAGGTAGGTAGAGATCATTGTTTTGTGGAAATGTAGTGGGGTTTTACCCTTTTTGATGTGTATAATTTGAATGATTGCTTACTCTAGTAAAGAACACTATGCTTATACCTATATGGCATGCAACTAAACAGTGGATCGACCCTAGTGTATTTTGACTTCCCAACATGAATATATAAAATTATGCTCTTATTTTTTCT
TARGET=515,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2722
PRIMER_LEFT_PENALTY=0.035021
PRIMER_RIGHT_PENALTY=0.237199
PRIMER_LEFT_SEQUENCE=CTTAAACCCAAGGAACTTGTGC
PRIMER_RIGHT_SEQUENCE=CCCACTACATTTCCACAAAACA
PRIMER_LEFT=298,22
PRIMER_RIGHT=554,22
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=59.763
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.2873
PRIMER_LEFT_1_PENALTY=0.050058
PRIMER_RIGHT_1_PENALTY=0.237199
PRIMER_LEFT_1_SEQUENCE=TTGGGTCCCTATTTTTAAGGGT
PRIMER_RIGHT_1_SEQUENCE=CCCACTACATTTCCACAAAACA
PRIMER_LEFT_1=451,22
PRIMER_RIGHT_1=554,22
PRIMER_LEFT_1_TM=59.950
PRIMER_RIGHT_1_TM=59.763
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=104
PRIMER_PAIR_PENALTY_2=0.2873
PRIMER_LEFT_2_PENALTY=0.050058
PRIMER_RIGHT_2_PENALTY=0.237199
PRIMER_LEFT_2_SEQUENCE=TGGGTCCCTATTTTTAAGGGTT
PRIMER_RIGHT_2_SEQUENCE=CCCACTACATTTCCACAAAACA
PRIMER_LEFT_2=452,22
PRIMER_RIGHT_2=554,22
PRIMER_LEFT_2_TM=59.950
PRIMER_RIGHT_2_TM=59.763
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.2876
PRIMER_LEFT_3_PENALTY=0.050443
PRIMER_RIGHT_3_PENALTY=0.237199
PRIMER_LEFT_3_SEQUENCE=ATCTGTACAGCGGGGTGTTACT
PRIMER_RIGHT_3_SEQUENCE=CCCACTACATTTCCACAAAACA
PRIMER_LEFT_3=259,22
PRIMER_RIGHT_3=554,22
PRIMER_LEFT_3_TM=59.950
PRIMER_RIGHT_3_TM=59.763
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.3010
PRIMER_LEFT_4_PENALTY=0.063793
PRIMER_RIGHT_4_PENALTY=0.237199
PRIMER_LEFT_4_SEQUENCE=CTTGGGTCCCTATTTTTAAGGG
PRIMER_RIGHT_4_SEQUENCE=CCCACTACATTTCCACAAAACA
PRIMER_LEFT_4=450,22
PRIMER_RIGHT_4=554,22
PRIMER_LEFT_4_TM=60.064
PRIMER_RIGHT_4_TM=59.763
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_01707 Ps_contig_mira-and-tgicl-ass_franssen_15905
SEQUENCE=ATAATGTATAACAAATTGGGATAAAAACACCAGTTAACTARCAAACAGACCATCCATACAAATTATCTCGAATAAAATCAAGAGAGGTGCATAATTTACATAAAATTACCAGGGCACATGAGTCAGATATTACTATCTCAAAATCCTTGTGCAATTCTAGAAGCAAAACACATTAAAACCAGTAACTTAAATAGTCTGAGAATACTGTTTAGACACTGCCATTCTCACTGGCTGGATCCTGGAACTGGAGCAGTATCTTCAATAGCCATTGGCTCAGTCTGAGGAGCTTTCTTCGTAAGCAATTTTGGGATGTCAACATTATCTTGTACACAGCGCTGTTGAGGATGCGACAATAGAATTAACACATGACGGAAAAGAACCCTAGCTAACCCTTGTCCACTCTCCCAGATATAACCCTCGTATTCAGTAACATGTTCAAGAGTTTCCACTAATGTATCATACACCTCCTGGGGGCAATTGTCAGGTCCGGGTTTGTCATGAAGGTACATGACATCCAAAGTGAATATCTTCTGGTTCACAGGCCACTGATGCAGCGGACGCACGTTTGATCGGCAGTAGAATAAAATCACTCTTAAAATGCGACCCTGCGCACGGGATTTTCTAGATTCATGAGTAGCAAGTCGAAACAAAGTAGTAAGGTCTGGTTGACTGCTAGATTTAGTAGCTGTCAGCGCTCGCATTGCTGCAATAGTTGACTCAACTTCACTGCTAAAATCTTTCTTCAACCATGAGACAGAACTGGAGAGAGTAGCAAAAGCAAACCGATGGTGAGGATTGATGGTGAGTTTGGCGTTGACAAAGAGAATGATGGCTTGCTTAATGGCATCTAAACGGGTGAGGGGTCTTCCATTAGGACCAGTAGCACCCTTCATCTCAGCCATGGATTGAGGATCAATATCCACACAGAATAGGATATCTTCATTGTTGATTCGAGAGGGTTGCAGAGTGTACTTGCTGCTGCTGCTTCCCAAACCCTTATGGCTGCTTTCTCCTTCAATCCCTTCCATCTGTCTTTCTTTCTTTCAGGTTCTAACACTGTAATGTAATGAGTAAAATAATAGAGGAACTAACCCCTTTAAATTAATTGTCTCCTTTTCTACCTTTCCAATTCAGATTAACCTTGTATACTTTTTCTGTCCTAATACAACACATACCATACCGATTCTTTTTTTTATTGCTTCTTAGAGAATAGAGAGGGAGTCTTGGTTCGGAGTAAAGGASACCCCCGAC
TARGET=974,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01708 Ps_contig_mira-and-tgicl-ass_franssen_15905
SEQUENCE=ATAATGTATAACAAATTGGGATAAAAACACCAGTTAACTARCAAACAGACCATCCATACAAATTATCTCGAATAAAATCAAGAGAGGTGCATAATTTACATAAAATTACCAGGGCACATGAGTCAGATATTACTATCTCAAAATCCTTGTGCAATTCTAGAAGCAAAACACATTAAAACCAGTAACTTAAATAGTCTGAGAATACTGTTTAGACACTGCCATTCTCACTGGCTGGATCCTGGAACTGGAGCAGTATCTTCAATAGCCATTGGCTCAGTCTGAGGAGCTTTCTTCGTAAGCAATTTTGGGATGTCAACATTATCTTGTACACAGCGCTGTTGAGGATGCGACAATAGAATTAACACATGACGGAAAAGAACCCTAGCTAACCCTTGTCCACTCTCCCAGATATAACCCTCGTATTCAGTAACATGTTCAAGAGTTTCCACTAATGTATCATACACCTCCTGGGGGCAATTGTCAGGTCCGGGTTTGTCATGAAGGTACATGACATCCAAAGTGAATATCTTCTGGTTCACAGGCCACTGATGCAGCGGACGCACGTTTGATCGGCAGTAGAATAAAATCACTCTTAAAATGCGACCCTGCGCACGGGATTTTCTAGATTCATGAGTAGCAAGTCGAAACAAAGTAGTAAGGTCTGGTTGACTGCTAGATTTAGTAGCTGTCAGCGCTCGCATTGCTGCAATAGTTGACTCAACTTCACTGCTAAAATCTTTCTTCAACCATGAGACAGAACTGGAGAGAGTAGCAAAAGCAAACCGATGGTGAGGATTGATGGTGAGTTTGGCGTTGACAAAGAGAATGATGGCTTGCTTAATGGCATCTAAACGGGTGAGGGGTCTTCCATTAGGACCAGTAGCACCCTTCATCTCAGCCATGGATTGAGGATCAATATCCACACAGAATAGGATATCTTCATTGTTGATTCGAGAGGGTTGCAGAGTGTACTTGCTGCTGCTGCTTCCCAAACCCTTATGGCTGCTTTCTCCTTCAATCCCTTCCATCTGTCTTTCTTTCTTTCAGGTTCTAACACTGTAATGTAATGAGTAAAATAATAGAGGAACTAACCCCTTTAAATTAATTGTCTCCTTTTCTACCTTTCCAATTCAGATTAACCTTGTATACTTTTTCTGTCCTAATACAACACATACCATACCGATTCTTTTTTTTATTGCTTCTTAGAGAATAGAGAGGGAGTCTTGGTTCGGAGTAAAGGASACCCCCGAC
TARGET=1032,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01709 Ps_contig_mira-and-tgicl-ass_franssen_15907
SEQUENCE=SAGACTCAAAATTTTCTGGTATATATATATGTCATGAAGAATGCAACTCTTCATCTCACTATTTTATTTACGTACAATATCATTGGTTTATTAAGAAATTGTTGTGMYTAATTGAAGAATGACAAAAGACTATTTGATTGCCATAGGAACCTAACAAGCTTACACATGACTAAATTACTTACATTAGAACTAATTTAAACCAGGTAAGTATTTTAGGAAACGGTTTCCTAGTAACTTTACCTAAA
TARGET=20,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01710 Ps_contig_mira-and-tgicl-ass_franssen_15908
SEQUENCE=AGACTACTAAAATTTTCTGGTATATATATATGTCATGAAGAATGCAACTCTTCATCTCACTATTTTATTTACGTACAATATCATTGGTTTATTAGAAATGTTGTGCTTAATTGAAGAATGACAAAAGACTATTTGATTGCCATACGGAAACCTAACAAGCTTCACATGACTTAAATTATTACATTGAACTAATTAACAGAGWAKTTA
TARGET=21,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01711 Ps_contig_mira-and-tgicl-ass_franssen_15919
SEQUENCE=AGTTATTAATTCTATTTTGTAATTAAGGAAGAAATTAACAGAATCAACATCAAGTTAAAAAAAGTCTARCWGAATTACTAACAAATACAAACAGAACCAACTAAATGCAACTTAATGATTCACAGTGAATTTCAGATATTTGATATGAATTCCAATTTCATTTGRATGATAATTCCTAACTAACTATCAAATTTGAGAGGAATTTCATTGTGAATTTCAACCTAACTTCTAACCAACTCAGAATGCTATGAAAATTTACCGTACTAAGATTCTGCAGTTTCTTCAAAAACTTCAAACTCTCCAATCCTTCCCTCTTTCAGTCTCGTTTTCTTCACCTTCTCGCAATCGATTCATCATGCATTTCCATTTCAGATCCTCACGCCGTACATCGATCCACAACCTCACAACAAAAGCGGTTCTACTGTCGCCGATTGTCGTCGCGTCGGAGTTCCATTCCGGTAAGACGCAACGGTCTCGGCGATTCCGCCGTGGCTGTGAATAAGGTTTCTTGTTGTTTGGTTGTGTATTTTGTCGGACAGCCAATGGTTTATTGTGAGGCGAAGGAAAGGGGTTTCTGGAGGATGAGGTTTCGTGGAGGGAGCTCGTCGATAGTGATGTTGTTATGTGACGGTGTGGACGGCGATTAGGGGTGGGGAGACAAATTAGGTGGTTGTCGAAGTTGTTAGGTTATGAGTGGTGGTGCGGTTGCGAATGGAAATGAGCAGATTCACTGTGGTGCGGTGAAGGACGAACGACGTGCGTGGTTCTGTTTGTTGTTGTGGCTGTTCACGGTAGATGGTTGAGTGGCGACATCACAGAGGTCATGTGTAGTTTAAAGGAGTRATGRTGTGTAGTTGGGTTTGGTTGGTCCGGGTTTGGGGGGTTTTAGGGGTAAAGTTAGGTAAGGGAAGTTTCTTTGGTTGGTTGGTTGGAAGG
TARGET=917,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01712 Ps_contig_mira-and-tgicl-ass_franssen_15926
SEQUENCE=GTGGGAAGCTCAACCGGCTGACAATGGCAGTCCTGTAGCAGGCGCCCAGTATCACCAACCACCGCAAGGCGGTCATATGGCTATGACACATGTACAAAGTGCTGCAAATCATCAAATGCCTCAGGCAATGGGTTATGGCCAGGCTGGTGGTATGTATATGCAACCGAATGTGAACAATCATATGTCTGCAAACAATAACCATTTTGGAAGCAATCAGTTAGGCATGCACCCTCAGCACATGCAAGGTGGTGCAGGGCACTACAGTGGGTATGGCTTCACATCAAATGCAAGGTGGTCCTGCAAGTTTACCATAGTAATCCACAACAGATGTATGGAAACCATATGTAGGTTANGGCTACGGTCAACAACAACAACAACAACACGGAGTTCA
TARGET=363,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01713 Ps_contig_mira-and-tgicl-ass_franssen_15927
SEQUENCE=ATTTCCTATAAAATTTAGTTAAATTCAAACATGACATTAACTATTAGGAAATCTTTATCATTCTATCCAATGCCAGTCTTCCCATTTATTTCAATATCAATACAAGAAAAGGACAACTGAATCAACCAAAGAAAATGGAGCAAACGAAAGTAAGACCTCCATATTTTAATGCTTCCACCCTTCTCTCTTTCTCCACCCTTCTCCGTTTTCCATTTTTCAATGTTTTCTCTAGAATAAATTTACATGAATGCTAATCCCCCAGACAAGCAAACAATATGAAACAAACTATGTATATAAAACAAAAACATCACAAATTAGCAAAAGGCTAAAATGCAAACCAAGAGAGTTTTTACATGCTACCAGCTCGAGCTGGAGATGGTTTTGGCTTCACTTTGGCCATGTTCACAAGGTCCCCAAATAACTTATCCTCTGGCTTGGACGGCTTCCCTGATGGAACATAAGATGCAGAAATCTGATTAGAGTTTCGTAAATAACTATCATCTCTTACAGACATTCCATGCATTTGCTGCTCAATGTATGGAACTCCGTGTTGTTGTTGTTGTTGTTGACCGTAGCCATAACCTACATATTGGTTTCCATACATCTGTTGTGGATACATGGAAACTGCAGGACCACCTTGCATTTGATGTGAAGCCATACCCATGTAGTGCCCTGCACCACCTTGCATGTGCTGGAGGGTGCATCGCCTAACTGATTGCCTTACCAAAATTGGTTATTGTT
TARGET=549,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5121
PRIMER_LEFT_PENALTY=0.225403
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=TTGCTGCTCAATGTATGGAACT
PRIMER_RIGHT_SEQUENCE=TACATGGGTATGGCTTCACATC
PRIMER_LEFT=523,22
PRIMER_RIGHT=666,22
PRIMER_LEFT_TM=59.775
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=0.5456
PRIMER_LEFT_1_PENALTY=0.258908
PRIMER_RIGHT_1_PENALTY=0.286660
PRIMER_LEFT_1_SEQUENCE=TTCACAAGGTCCCCAAATAACT
PRIMER_RIGHT_1_SEQUENCE=TACATGGGTATGGCTTCACATC
PRIMER_LEFT_1=401,22
PRIMER_RIGHT_1=666,22
PRIMER_LEFT_1_TM=59.741
PRIMER_RIGHT_1_TM=59.713
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.5456
PRIMER_LEFT_2_PENALTY=0.258908
PRIMER_RIGHT_2_PENALTY=0.286660
PRIMER_LEFT_2_SEQUENCE=TCACAAGGTCCCCAAATAACTT
PRIMER_RIGHT_2_SEQUENCE=TACATGGGTATGGCTTCACATC
PRIMER_LEFT_2=402,22
PRIMER_RIGHT_2=666,22
PRIMER_LEFT_2_TM=59.741
PRIMER_RIGHT_2_TM=59.713
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.5650
PRIMER_LEFT_3_PENALTY=0.278329
PRIMER_RIGHT_3_PENALTY=0.286660
PRIMER_LEFT_3_SEQUENCE=TGCTGCTCAATGTATGGAACTC
PRIMER_RIGHT_3_SEQUENCE=TACATGGGTATGGCTTCACATC
PRIMER_LEFT_3=524,22
PRIMER_RIGHT_3=666,22
PRIMER_LEFT_3_TM=60.278
PRIMER_RIGHT_3_TM=59.713
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=0.5692
PRIMER_LEFT_4_PENALTY=0.225403
PRIMER_RIGHT_4_PENALTY=0.343828
PRIMER_LEFT_4_SEQUENCE=TTGCTGCTCAATGTATGGAACT
PRIMER_RIGHT_4_SEQUENCE=GTAAGGCAATCAGTTAGGCGAT
PRIMER_LEFT_4=523,22
PRIMER_RIGHT_4=723,22
PRIMER_LEFT_4_TM=59.775
PRIMER_RIGHT_4_TM=59.656
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=201
=
PRIMER_SEQUENCE_ID=SSR_01714 Ps_contig_mira-and-tgicl-ass_franssen_15933
SEQUENCE=ATATCAAATGGGAGATGAAGCGACAATAGATGAAGTCATAGATGATGTAGACACTGACAATGATGGGCGAATTAATTATCAGGAGTTTGTAGCTATGATGAAAAAGGGGACCTTGGRTCATGATGAGAGAGAGAAACCATGATAATTTTGTGATGTACGTACTAAATAAAAAAACATTGTTGTTTTCTGTATTTTATTGCTGCATTCTTGGTTTTCACAGAGGTNAGTCACTCTCGAATCTGT
TARGET=125,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01715 Ps_contig_mira-and-tgicl-ass_franssen_15934
SEQUENCE=TGCCTTGAANACCCACTGGATGAAGTAAGGTAGGTGAGGCATCTAGATAAGCCCTTAGACAATAGCTGTTTTAATTAGGATGAAACAGTTCAGAGCAATGAACAAGATGAAGAAACTTGCTTTAAAGGTAATAGCAGAAAACCTTGCAGAGGAAGAAATCAAGGGCTTGAAGCAAATGTTCAACAATATAGATACAGATCGAAGTGGCACAATCACATATGAAGAACTAAAATCTGGACTGTCCAAATTGGGATCCAAGCTTAGTGAATTTGAAATTAAGCAGCTAATGGAGGCTGCTGATGTTGACCAGAATGGAACTATTGACTATCATGAATTCATTACTGCCACTATAAACCGGCATAAACTTGAGAGGGAAGAGAACTTGTTCAAGGCTTTTCAATACTTTGACAAGGATAACAGCGGTTATGTAACAAGAGAGGAACTTAGACAAGCTTTGGCTGAATATCAAATGGGAGATGAAGCGACAATAGATGAAGTCATAGATGATGTAGACACTGACAATGATGGGCGAATTAATTATCAGGAGTTTGTAGCTATGATGAAAAAGGRSCTTGGNTCATGATGAGAGAGAGAAACCATGATTAATTTTTGTGATCGTACGTACTAAATAAAAAACATGTTGTTTTC
TARGET=585,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01716 Ps_contig_mira-and-tgicl-ass_franssen_15936
SEQUENCE=AGGACAAGATATACTAAGTCTACCCAAATGACAKACGGTCACTTTTCCAAAATATAGAAATCAGATCTCTCAAAACCTATTTCATGGATACCGTAGCTTCTCTAAAAATCTATGGTGCCATCGTTCAACCCTCTCTCTAGAACACTAGATTGTCTCTCTCTCACACACACATAGTAATCAATCAAACACCATACCATTCCTCTCTCAATATTTCTCTCTACCCCTCTTTCGGTTGCGAAATCGCTCAGGGATCTCTCACTTGCGGGAAGACACTCTTGCTCAGTTGATTTTCTCTCTCTATCAAGTTTGTCTGGTTTTCTTAGGTAGTTTTGGCTCCATTTTGGGTGGCTCTGGCATAGCAAGTTGTTGTGTCGTTGTTGTTTAAGTAAAGTAGGGGTTTGAGCTGATTTTGCAGGGTTGTC
TARGET=153,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01717 Ps_contig_mira-and-tgicl-ass_franssen_15956
SEQUENCE=AAATACTTCCCACTACTACAGCAAGCTCAAGCATGAGACGGTTTGCAATTTTAGACCATACACTTGTCCTTACGCTGGATCAGAGTGCTCCGCTGCTGGTGATATTAACTTCCTTGTCGCACATTTGAGGGATGATCATAAGGTCGACATGCACACAGGATGCACATTCAACCATCGTTATGTAAAGTCAAATCCGCGCGATGTCGAGAATGCTACTTGGATGCTCACTGTATTTCATTGTTTCGGCCAATACTTCTGCCTTCACTTTGAAGCTTTCCAGCTAGGCATGGCTCCAGTTTACATGGCATTCCTCCGTTTCATGGGAGACGAGAACGATGCTAGAAACTACACATATAGCCTCGAAGTTGGAGCCAATGGTAGAAAACTCATTTGGGAAGGTACACCACGGAGTATCCGCGACAGCCATCGTAAAGTCCGAGATAGTCACGACGGACTCCTAATTCAACGAAATATGGCTCTATTTTTCTCTGGTGGTGATAGGAAAGAACTGAAACTAAGAGTGACAGGAAGAATATGGAAAGAACAACAGAATCCAGATGGTGGGGTGTGTATACCAAATCTTTGCAGCTAATGTGGGTTTTTATTGTCTGTGTGTGATGTATGTATATCTACACTACACTACACTACACCAACTTTGTGTATGTTATGTAAACTCTAATAGATTAAAGTACTAAGATAATGGCTTTGAAATTTTCAGTTTTTCCAAGTTGTGTAATAAAACAAGATTTGGAACTATGATTTAGACCAGTGTTGGAGCATTTTCATATAAAGAGTTTACAGAAGAATTACACGC
TARGET=630,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0313
PRIMER_LEFT_PENALTY=0.027715
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=ATCTTTGCAGCTAATGTGGGTT
PRIMER_RIGHT_SEQUENCE=TATGAAAATGCTCCAACACTGG
PRIMER_LEFT=578,22
PRIMER_RIGHT=786,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.0313
PRIMER_LEFT_1_PENALTY=0.027715
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=AATCTTTGCAGCTAATGTGGGT
PRIMER_RIGHT_1_SEQUENCE=TATGAAAATGCTCCAACACTGG
PRIMER_LEFT_1=577,22
PRIMER_RIGHT_1=786,22
PRIMER_LEFT_1_TM=60.028
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.2242
PRIMER_LEFT_2_PENALTY=0.220659
PRIMER_RIGHT_2_PENALTY=0.003589
PRIMER_LEFT_2_SEQUENCE=CAAATCTTTGCAGCTAATGTGG
PRIMER_RIGHT_2_SEQUENCE=TATGAAAATGCTCCAACACTGG
PRIMER_LEFT_2=575,22
PRIMER_RIGHT_2=786,22
PRIMER_LEFT_2_TM=59.779
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.2242
PRIMER_LEFT_3_PENALTY=0.220659
PRIMER_RIGHT_3_PENALTY=0.003589
PRIMER_LEFT_3_SEQUENCE=CCAAATCTTTGCAGCTAATGTG
PRIMER_RIGHT_3_SEQUENCE=TATGAAAATGCTCCAACACTGG
PRIMER_LEFT_3=574,22
PRIMER_RIGHT_3=786,22
PRIMER_LEFT_3_TM=59.779
PRIMER_RIGHT_3_TM=59.996
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.5229
PRIMER_LEFT_4_PENALTY=0.519311
PRIMER_RIGHT_4_PENALTY=0.003589
PRIMER_LEFT_4_SEQUENCE=CTTTGCAGCTAATGTGGGTTTT
PRIMER_RIGHT_4_SEQUENCE=TATGAAAATGCTCCAACACTGG
PRIMER_LEFT_4=580,22
PRIMER_RIGHT_4=786,22
PRIMER_LEFT_4_TM=60.519
PRIMER_RIGHT_4_TM=59.996
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_01718 Ps_contig_mira-and-tgicl-ass_franssen_15965
SEQUENCE=TCGGGGGACTAAAAATACACACAAAAAAAAGAGAGAAAAATCACAAGTGACGGACGACGACTTCAGTTGAAACAGTTTCAAGTTATAGAGTTTCGAGAGAGAGAGAGTGAAGCGAAGGCAGAGAAGAGAATGGCAAAGACATCTTCCACCACGAAGGATGCACAAGATCTATTCCACGCTATTTGGTCTGCATATTCCGCCACACCTACAAATCTCAAGATCATCGATCTCTATGTTGTCTTCGCTGTTTTCACCGCACTCCTTCAGGTAGTTTACATGGCTCTGGTTGGAACATTTCCATTTAACTCCTTCCTTTCTGGAGTACTCTCTTGTGTAGGAACCGCGGTTCTGGCTGTTTGTCTCCGGATCCAAGTCAACAAAGAGAACAAGGAATTCAAGGATCTTGCGCCCGAGCGTGCTTTTGCTGATTTTGTTCTCTGCAATTTGGTGCTTCATTTGGTGATCATGAACTTCCTTGGTTAAATTGGTTTGGTGCAGTTTTGTTATTTCCAAGAGAACCCTCGGTTGATAAAGATCAATAGTATTACATGAGTTTTATGAAACAGTATTTAAAGACAGTCACTGAGATTTAGCAAATTAATGTAGTTTCTTGTTGAGATGCTAGATATTTTTCTCTTTTAAGCTATGTTATTATTGATGAATTACAGTTTTTTCACATGGATATCTAATCCTCGTTACTACTACCAAAAACTAAAGTTTAATGTTTGTTGGGAGAAA
TARGET=95,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1904
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.081468
PRIMER_LEFT_SEQUENCE=TCGGGGGACTAAAAATACACAC
PRIMER_RIGHT_SEQUENCE=GGAAGATGTCTTTGCCATTCTC
PRIMER_LEFT=0,22
PRIMER_RIGHT=146,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=147
PRIMER_PAIR_PENALTY_1=0.6046
PRIMER_LEFT_1_PENALTY=0.334292
PRIMER_RIGHT_1_PENALTY=0.270338
PRIMER_LEFT_1_SEQUENCE=GACGACGACTTCAGTTGAAACA
PRIMER_RIGHT_1_SEQUENCE=TAGATCTTGTGCATCCTTCGTG
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=170,22
PRIMER_LEFT_1_TM=60.334
PRIMER_RIGHT_1_TM=60.270
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=119
PRIMER_PAIR_PENALTY_2=0.7613
PRIMER_LEFT_2_PENALTY=0.108929
PRIMER_RIGHT_2_PENALTY=0.652369
PRIMER_LEFT_2_SEQUENCE=TCGGGGGACTAAAAATACACAC
PRIMER_RIGHT_2_SEQUENCE=TAAACTACCTGAAGGAGTGCGG
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=274,22
PRIMER_LEFT_2_TM=60.109
PRIMER_RIGHT_2_TM=60.652
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=11.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.8192
PRIMER_LEFT_3_PENALTY=0.108929
PRIMER_RIGHT_3_PENALTY=0.710314
PRIMER_LEFT_3_SEQUENCE=TCGGGGGACTAAAAATACACAC
PRIMER_RIGHT_3_SEQUENCE=GTGAAAACAGCGAAGACAACAT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.290
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.8630
PRIMER_LEFT_4_PENALTY=0.781508
PRIMER_RIGHT_4_PENALTY=0.081468
PRIMER_LEFT_4_SEQUENCE=GAGAAAAATCACAAGTGACGGA
PRIMER_RIGHT_4_SEQUENCE=GGAAGATGTCTTTGCCATTCTC
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=146,22
PRIMER_LEFT_4_TM=59.218
PRIMER_RIGHT_4_TM=60.081
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_01719 Ps_contig_mira-and-tgicl-ass_franssen_15968
SEQUENCE=AGTTAACATAGGTAATTGGTTATATTTATATTATATATATACTGGTATTGTGGTTTTGTCCATTCTGTCAAGTATACACAATTGTAGGTAGATATGATTTTTTTACAAAAATGCATCACGTATTAATCGCGCGAATACCCGAGAAGATAATCTGCTAAAAACATCAGGAAGAACTTTAGTAGAGATACCCGGCAACACTGGCAGAAGTTCTTGGATAATCTGAGGAACATTGAGTGCCTGACTAGATGTGCTTGACAGAGAACTTCCTGCAGTCAAGAATTCCAAGACTTTCTGGACATTGTTTAGTATAACCTTATCCTCTTCGGTTATGGTGGGTATAAGAGCTGCGGGTTTGAAACGTCCAATAGTAGGTACCATATTGAAAATAGGGATTGCATTCTGAACTCCTAGTAAGGACATTATTTTTACCAGTTGTTCCCTAGTTACTGCATCAATGCCCTTAACGATCTCATCCAACAGAAATTCTCGGAAAAAATTCCCCTTATCAGACAGCAAAAACGCTAGGGCTGCCCTTGTTTCCACTGGCTGCTGTTGAGGTATCACAGACTGGAATCCTGGCAAGAGATATTCTGACCTGTTAGAAACAATCCCAAGCTCAGCCATATCCCCTTTCAGATCCTCTCCACCTCCACTTTTGGCAGCTGTAATAAAATTCTCGAAGGCTTGCATGACATCTATAAATCTTTCAGCATCAAAAACTCCAGATTTCCCATATATAGTGTAACGTAGAGCACTCCTTAGCCGAGGGGATTCATCCGTCAGAAGCCTCTGTGCAATATATGGATAGGCTTCATCAACAATGGCAAAATCAGGATTTCCAACTAAAGCTATTCCTTCAAGAACCCCGATAGCTCTGATTATAAGAGCAAAGTATGGGGGTATCCTAAAAGGATAATCAAAGGTAATTTGAGCTAAATCTGATGCCAGATCTTGAAAATTTATGTTTTTTGCCCCTCCACCTTCTAAAGCCTGGTCAAATACTTTGGCAAGCACTGGCAAGATAGGCTCTAAATTGACTCCATCTGGAAYGAWACCAAGTTTAACAAAGTCTCTCACTATGGCTGTATAAT
TARGET=32,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01720 Ps_contig_mira-and-tgicl-ass_franssen_15988
SEQUENCE=GCAGCGAGGACAGTTCAAGGCTTGGTTGTTGTTCTGGTTGTTGTTGTTGAGATGTGGCCTGAGTCTCCGGTCGCCGCCTCCGCCGCCGAAGAACCTGGTACCGCCAGCGCCGATGGAATGTATTTCTTGCATTTGAGGTTGGTTTGGGGATTGAGGAAGCAATGAAAGGATCACCTGAGAGTGGTGTGTGTGTCAGTGTGTTAATGTTAATATGTGAGGAGTGTGGAGAAGAAGGGAGGGAAAGGGAGAGAGAAAGAGAAAGGAGTTTCGATTGGGTGTTGTC
TARGET=37,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1110
PRIMER_LEFT_PENALTY=0.074807
PRIMER_RIGHT_PENALTY=0.036173
PRIMER_LEFT_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_RIGHT_SEQUENCE=ACACCACTCTCAGGTGATCCTT
PRIMER_LEFT=11,22
PRIMER_RIGHT=186,22
PRIMER_LEFT_TM=60.075
PRIMER_RIGHT_TM=60.036
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.1563
PRIMER_LEFT_1_PENALTY=0.074807
PRIMER_RIGHT_1_PENALTY=0.081468
PRIMER_LEFT_1_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_RIGHT_1_SEQUENCE=GTGATCCTTTCATTGCTTCCTC
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=173,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.1951
PRIMER_LEFT_2_PENALTY=0.074807
PRIMER_RIGHT_2_PENALTY=0.120245
PRIMER_LEFT_2_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_RIGHT_2_SEQUENCE=GACACACACACCACTCTCAGGT
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=193,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=60.120
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=0.3626
PRIMER_LEFT_3_PENALTY=0.074807
PRIMER_RIGHT_3_PENALTY=0.287765
PRIMER_LEFT_3_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_RIGHT_3_SEQUENCE=CCTTCTTCTCCACACTCCTCAC
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=234,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=60.288
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.3837
PRIMER_LEFT_4_PENALTY=0.074807
PRIMER_RIGHT_4_PENALTY=0.308871
PRIMER_LEFT_4_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_RIGHT_4_SEQUENCE=TCTTTCTCTCTCCCTTTCCCTC
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=256,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=60.309
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_01721 Ps_contig_mira-and-tgicl-ass_franssen_15988
SEQUENCE=GCAGCGAGGACAGTTCAAGGCTTGGTTGTTGTTCTGGTTGTTGTTGTTGAGATGTGGCCTGAGTCTCCGGTCGCCGCCTCCGCCGCCGAAGAACCTGGTACCGCCAGCGCCGATGGAATGTATTTCTTGCATTTGAGGTTGGTTTGGGGATTGAGGAAGCAATGAAAGGATCACCTGAGAGTGGTGTGTGTGTCAGTGTGTTAATGTTAATATGTGAGGAGTGTGGAGAAGAAGGGAGGGAAAGGGAGAGAGAAAGAGAAAGGAGTTTCGATTGGGTGTTGTC
TARGET=184,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3626
PRIMER_LEFT_PENALTY=0.074807
PRIMER_RIGHT_PENALTY=0.287765
PRIMER_LEFT_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_RIGHT_SEQUENCE=CCTTCTTCTCCACACTCCTCAC
PRIMER_LEFT=11,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=60.075
PRIMER_RIGHT_TM=60.288
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.3837
PRIMER_LEFT_1_PENALTY=0.074807
PRIMER_RIGHT_1_PENALTY=0.308871
PRIMER_LEFT_1_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_RIGHT_1_SEQUENCE=TCTTTCTCTCTCCCTTTCCCTC
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=256,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=60.309
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=0.3837
PRIMER_LEFT_2_PENALTY=0.074807
PRIMER_RIGHT_2_PENALTY=0.308871
PRIMER_LEFT_2_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_RIGHT_2_SEQUENCE=TCTCTTTCTCTCTCCCTTTCCC
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=258,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=60.309
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=0.3903
PRIMER_LEFT_3_PENALTY=0.081468
PRIMER_RIGHT_3_PENALTY=0.308871
PRIMER_LEFT_3_SEQUENCE=GAGGAAGCAATGAAAGGATCAC
PRIMER_RIGHT_3_SEQUENCE=TCTTTCTCTCTCCCTTTCCCTC
PRIMER_LEFT_3=152,22
PRIMER_RIGHT_3=256,22
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=60.309
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.3903
PRIMER_LEFT_4_PENALTY=0.081468
PRIMER_RIGHT_4_PENALTY=0.308871
PRIMER_LEFT_4_SEQUENCE=GAGGAAGCAATGAAAGGATCAC
PRIMER_RIGHT_4_SEQUENCE=TCTCTTTCTCTCTCCCTTTCCC
PRIMER_LEFT_4=152,22
PRIMER_RIGHT_4=258,22
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=60.309
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=107
=
PRIMER_SEQUENCE_ID=SSR_01722 Ps_contig_mira-and-tgicl-ass_franssen_15991
SEQUENCE=TATCTTCAAAGTTGATGCACTGCTTTTACTAACCCACACAAATGTTTATTTCATTATTTAAGATTTTTTGTTGCTGTGCTGGATTTTCCTCTAGCTTATCGAAATGAGGTTTTGTTTGTGTCTTTTTTAGTAAGAGGACGAGGGGTGCATGCATGCAAATGCAAAGAACCACAGAGGATGAGGAGGAGAACAAGAGGAAGAGAAGCCATGTTGAGATTAAAGGTTTTWTGAGTCTTTTTTCGTAGGGGTAAGGTTTGGTTTCACTTATAATGGTAGAATAGAAGGTGCATTGTTTTGGAAAAACAGAGAAAAGAATAGAAGGTGCATTAAATGT
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01723 Ps_contig_mira-and-tgicl-ass_franssen_15992
SEQUENCE=TCGGGGTATGGCTTCTCTTCSKSKKGTTCTCCTCCTCATCCTCTGTGGTTCTYTGCATTTGCATGCATGCACCCCTCGTCCTCTTACTAAAAAAGACACAACAAAACCTCATTTCGATAAGCTAGAGGAAAATCCAGCACAGCAACAAAAAATCTTAAATAATGAAATAAACATTTGTGTGGGTTGTAGCAGTGCGTCAACTTTGAAGATAGTGATCGTAGGGTCATCTTTCCATACGGGTTCGCCATT
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01724 Ps_contig_mira-and-tgicl-ass_franssen_15998
SEQUENCE=CGCGCATTGAAGGGGTTAGTGAGGCTGCAGGCGTTAGTGAGGGGACACAATGTGCGGAAGCAAGCTCAAATGACGATGCGGTGTATGCAAGCATTGGTGCGGGTGCAGGCGCGAGTGAGAGCGCGACGACTTCAATTGAGCCACGAAAATTTTGAGAAGGAAATGGATGAGTATGAAGAAGAAGAATTTGTGAGACAACAAGTGAGTTTTAATAAGCCTATGAGTCCGATGAGAAGGTCATCAGGCATTAATATTAATAATAATGGTTTGGATCATAACAAACGTCAAAGTAGACAAAAAAGACTAAAGAAAGTGATTTGAAAAAACATGAAGCTGACAATGAAGAGGGAAAGAGCTCTTGCATATGCTTTCAATTATCAACAGCAACAACAAAAGCAACATCTACTACATAGAGATAAGATGGCGAAGNTGTTGTTATGAAGAAACTNTGATTCTAATGATAATAGAAAATCCAAGTGGGTTGAATTTGGTTGGAGCGTT
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2728
PRIMER_LEFT_PENALTY=0.236697
PRIMER_RIGHT_PENALTY=0.036112
PRIMER_LEFT_SEQUENCE=GAGCCACGAAAATTTTGAGAAG
PRIMER_RIGHT_SEQUENCE=ATGACCTTCTCATCGGACTCAT
PRIMER_LEFT=137,22
PRIMER_RIGHT=240,22
PRIMER_LEFT_TM=60.237
PRIMER_RIGHT_TM=59.964
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=0.4782
PRIMER_LEFT_1_PENALTY=0.236697
PRIMER_RIGHT_1_PENALTY=0.241544
PRIMER_LEFT_1_SEQUENCE=GAGCCACGAAAATTTTGAGAAG
PRIMER_RIGHT_1_SEQUENCE=TTTCCCTCTTCATTGTCAGCTT
PRIMER_LEFT_1=137,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=60.237
PRIMER_RIGHT_1_TM=60.242
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=215
PRIMER_PAIR_PENALTY_2=0.5428
PRIMER_LEFT_2_PENALTY=0.236697
PRIMER_RIGHT_2_PENALTY=0.306150
PRIMER_LEFT_2_SEQUENCE=GAGCCACGAAAATTTTGAGAAG
PRIMER_RIGHT_2_SEQUENCE=TGACCTTCTCATCGGACTCATA
PRIMER_LEFT_2=137,22
PRIMER_RIGHT_2=239,22
PRIMER_LEFT_2_TM=60.237
PRIMER_RIGHT_2_TM=59.694
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.6381
PRIMER_LEFT_3_PENALTY=0.236697
PRIMER_RIGHT_3_PENALTY=0.401361
PRIMER_LEFT_3_SEQUENCE=GAGCCACGAAAATTTTGAGAAG
PRIMER_RIGHT_3_SEQUENCE=ACCTTCTCATCGGACTCATAGG
PRIMER_LEFT_3=137,22
PRIMER_RIGHT_3=237,22
PRIMER_LEFT_3_TM=60.237
PRIMER_RIGHT_3_TM=59.599
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=0.8667
PRIMER_LEFT_4_PENALTY=0.236697
PRIMER_RIGHT_4_PENALTY=0.629991
PRIMER_LEFT_4_SEQUENCE=GAGCCACGAAAATTTTGAGAAG
PRIMER_RIGHT_4_SEQUENCE=CCCTCTTCATTGTCAGCTTCAT
PRIMER_LEFT_4=137,22
PRIMER_RIGHT_4=348,22
PRIMER_LEFT_4_TM=60.237
PRIMER_RIGHT_4_TM=60.630
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_01725 Ps_contig_mira-and-tgicl-ass_franssen_16004
SEQUENCE=GGTATCAACGCAGAGTACGRVGGGGTAAGGATCAACGCAAAGCAAAGCAAAGCAGTAAAAAAAACTTTCTCAAATCTTTTCTTCCATTGTCGCCGGGATGTCGCCGGTGATCGGAGCAAACGCACCTCTCTTGTCAGGATCCAAGACTTCTTTGAAACCGGCAACTGTTTCCGGCGCGGTGTTCAATGTCGCAACGAGCGTAATTGGCGCCGGAATCATGTCTCTTCCGGCAACTCTGAAGGTTCTCGGTGTGATTCCGGCTTTCGTCTTGGTTCTGGTAATTGCTTTACTGGCTGAAATTTCTGTTGAATTTTTAATGAGGTTTACGCGTTCGGGTGAAACGACGACGTATTCTGGTGTCATGAGAGAAGCTTTTGGTCCTTTAGGAGCTGCAGCAACTCAACTTTGTGTTGTGATTACTAATATGGGGTGTTTAATTATGTACCTAATTATCATGGCGGATGTATTTTCTGGAAATAAACCTGAAGGAGTAGTACATATGGGTGTTTTGCAACAATGGTTTGGGATTCATTGGTGGAATTCAAGAGAATTTGCTTTAATCTTTACCATGATCTTCATTTTGTTTCCATTGGTATTGTACCGTCGCGTTGAGTCATTGAAGTTCAGTTCTGCAGTATCAACTCTTCTGGCTGTGGCATTTGTTACAATATGTTCTGTGTTGGCAATCATTGCTCTCGCGGAAGGAAAAACGCAGACACCTAGACTGGTCCCTCGTTTGGACAAACAAACATCTTTCTTTGATCTTTTCACTGCAGTTCCGGTCATTGTAACAGCCTACACATTTCATTTTAATGTACATCCAATTGGATTTGAGCTTGGAAAACCTTCAGAAATGGCAAAAGCAGTTAGAATAGCACTAATCCTATGTGCAGTTATATACTTCTCAATAGGTCTATTTGGTTACCTTTTATTTGGTGATTCAACTCAATCAGACATTCTAATCAATTTCGACCACAGTGCTGATTCGTCCTTTTGGTCTATTTACTCAATTACTTTGGTTCCGTTTAAGT
TARGET=37,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01726 Ps_contig_mira-and-tgicl-ass_franssen_16014
SEQUENCE=TCGRGATGCACAGTTTTTACACTGGAGTGGTCAAACCTCAAAACAACAAACAACAACAACAATGCAAACATCAATCGCATTCTCTCCATTTCCAAACGCACTCATCAAATTCAGAACACAACCTTCCAAACCAAACCCTATTTCTCACCGAACCAAACCATCTTTTCCCATTACACTATGCAATTTTCAACCTCAATCACCCTCAACCCCCTCCACATTGAAAYTCCCCCTTAACCCGCGTCTCCAACGCGGGTCCTCTTCGTTGCGGCATTTCGTCGAACAGCGAGARCGCAAATGTGGGTGAGAGGAGTTTAAGGCAGTGGGTCGAGCTTACTGGTGAGGTGATATCGACGGCGTTTCCTTTGTGGGTTACCATTGGGTGTGTATTGGGTTTGGTGAGACCGAGTTCATTCACTTGGGTTACTCCTAAATTGAGCATTGTGGGTCTTAGTGTAATTATGCTTGGTATGGGTATGACTTTAACTCTTGATGATCTTCGCTCTGCTCTTTCTATGCCTAAAGAAGTCTTCTCTGGTTTTTTCCTTCAATATTCGGTGATGCCATTATCTGCGTTTTTTATTAAGCAAACTCTTAAATCTTCCCTCACATTATGCAGCTGGTTTAATATTAGTTGGGTGCTGTCCAGGAGGCACAGCTAGTAATATTGTTACATATCTTGCACGTGGAAATGTGGCGCTTTCCGTTGTAATGACAGCTGCAAGCACTCTATCAGCCGTGATCATGACTCCTTTTCTGACAGCCAAACTAGCTGGTAAATATGTAGCTGTGGATGCAGCTGGCTTATTAATGTCGACATTGCAGGTCGTGCTTTTTCCTGTATTAGCTGGTGCGCTTCTGAATCAGTATTTCCAACCTCTTGTTAAACTTGTATCTCCATTGATGCCACCCTTGGCCGTTGCAACTGTGGCAATTTTATGTGGAAATGCAATTTCACAGAGTTCTTCTGCAATCCTTATGTCTGGAAGACAGGTAATCTTAGCCGTCTGCCTTCTTCATGCTTCTGGTTTTTTCTTCGGGTACGTTCTTGCAAGGATTCTCGGGCTAGATATGCCATCATCGCGAACCATATCCATTGAAGTTGGCATGCAGAACTCGGTTCTCGGTGTAGTGCTGGCTGCCAAGCACTTTGGAGACCCCCTAACAACGGTACCTTGTGCTGTTTCAAGTGTGTGTCACTCAATCTTTGGTAGCATCTTGGCAGGAATGTGGAGACGTTCTGTGCCTCCTGAGATAAAGGACTGAACATAGTTAGCTAGAATAGCTAG
TARGET=49,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01727 Ps_contig_mira-and-tgicl-ass_franssen_16021
SEQUENCE=GGTAGAACTAACGCCTGAAAWMRRKGAAACCGAGGAAGAAAGAGAGGGCTTCGACTCAACGCACTTTCTCTCTTCCTCTTCTTCTTCTTCTTCCCACCCAAGTCATGAGCCTCTTCGGTATTGGCAGAAATCAGAGAACATTCCGACCCAAAAAAAGTCTCCTTCTGGAAGCAAGGGAGCTCAGCTTAAGAAAACACATTGATGACAACTAGTTAGGTAGTAGGAAATCGTAGGAAGGGGAAGACAAGTAAACGCCTACCCTCGTGGAGAGGTATTTAG
TARGET=77,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01728 Ps_contig_mira-and-tgicl-ass_franssen_16026
SEQUENCE=CAAATATCAATTGGGGAAAAGAAATTTATCCTGAAGATTCAATTTATAACCGCCGCAGGGGCGGAAGGAAAATTTTAACCAATTGCCAATTCAATAACAGTTTTACATATCCTTGTTGTCTTTTTCCCTCCCTTCGGTTCAATTCCTCCCCTGCAACTCAAAAAGGTATGTGGAGCCGACCAGTCCTTCAGATGAAAAAAAAGTCAGACAGAAACTAAGTTTGAGCAGAATACCAAACAGCAAAAGCATGTCACTTCAGGATTTCTTAGGCCTCTTAGAAGTTCCTTCCCTTTTATCGATCTCTGCAGACTTCTTCTTGTCCTTCCTTTCTCTCTTCTTCAGAGCAGTCATATGGGCCTTTAATATAGTTGCATAAGAAGCTTGAAAGCGTTGATGATCCTTTGGTCCAACCGAAGTAGAAATGGTCTTTTTCCCATCAGTGGCACGTATGAGACATCTATGCTCAATTGTCTCACCAGCAGTAACCAATTTATTCTTCTCAACTTTAGATTTCAAGGATGATCGTTTGAGAGTAACCCAAACAGAACCCTTGTCAGTGGTACGCTCGAACATGGCGGTGAGTTCATTGAGAAACGGATCTAATTGTAGCAAAACCATTGTTGAGAGAGAGAGAGAGAGTTTGGTGCCGAAAACGCAGTCGTATGGGAGAGAACGTTGAGAGACAGAGAGGAGTTTTTGGTGGCGAAAACGGTGTCGTATCCCGACT
TARGET=623,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3316
PRIMER_LEFT_PENALTY=0.185822
PRIMER_RIGHT_PENALTY=0.145769
PRIMER_LEFT_SEQUENCE=GAAATGGTCTTTTTCCCATCAG
PRIMER_RIGHT_SEQUENCE=CCACCAAAAACTCCTCTCTGTC
PRIMER_LEFT=418,22
PRIMER_RIGHT=702,22
PRIMER_LEFT_TM=59.814
PRIMER_RIGHT_TM=60.146
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.3668
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=0.145769
PRIMER_LEFT_1_SEQUENCE=AATGGTCTTTTTCCCATCAGTG
PRIMER_RIGHT_1_SEQUENCE=CCACCAAAAACTCCTCTCTGTC
PRIMER_LEFT_1=420,22
PRIMER_RIGHT_1=702,22
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=60.146
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.3834
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=0.254773
PRIMER_LEFT_2_SEQUENCE=TTGGTCCAACCGAAGTAGAAAT
PRIMER_RIGHT_2_SEQUENCE=GTCTCTCAACGTTCTCTCCCAT
PRIMER_LEFT_2=401,22
PRIMER_RIGHT_2=683,22
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=59.745
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.4002
PRIMER_LEFT_3_PENALTY=0.254467
PRIMER_RIGHT_3_PENALTY=0.145769
PRIMER_LEFT_3_SEQUENCE=TAACCCAAACAGAACCCTTGTC
PRIMER_RIGHT_3_SEQUENCE=CCACCAAAAACTCCTCTCTGTC
PRIMER_LEFT_3=533,22
PRIMER_RIGHT_3=702,22
PRIMER_LEFT_3_TM=60.254
PRIMER_RIGHT_3_TM=60.146
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=0.4241
PRIMER_LEFT_4_PENALTY=0.278329
PRIMER_RIGHT_4_PENALTY=0.145769
PRIMER_LEFT_4_SEQUENCE=TATGCTCAATTGTCTCACCAGC
PRIMER_RIGHT_4_SEQUENCE=CCACCAAAAACTCCTCTCTGTC
PRIMER_LEFT_4=458,22
PRIMER_RIGHT_4=702,22
PRIMER_LEFT_4_TM=60.278
PRIMER_RIGHT_4_TM=60.146
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_01729 Ps_contig_mira-and-tgicl-ass_franssen_16040
SEQUENCE=CGTATCAGCAACTATAAAACAAAACCAAAAAAACCTAAACTAAGTCATTTGTCTTCAAACAAAAAGTAACAAACAATTACTCTTCCATTCCATCATCATCATCATTATCATCATCATCATCATCATCATCATCATCATCATCATAGTGATATCTTATGCTCAGGTCTATGAGTGAACTCAAAATCAATGTGCACAAAAGCTCTTTCAACATCTGGAAGCTGTTCTAGTTTCTCTTGTTTCTTGTAATACTCCGCC
TARGET=91,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01730 Ps_contig_mira-and-tgicl-ass_franssen_16040
SEQUENCE=CGTATCAGCAACTATAAAACAAAACCAAAAAAACCTAAACTAAGTCATTTGTCTTCAAACAAAAAGTAACAAACAATTACTCTTCCATTCCATCATCATCATCATTATCATCATCATCATCATCATCATCATCATCATCATCATAGTGATATCTTATGCTCAGGTCTATGAGTGAACTCAAAATCAATGTGCACAAAAGCTCTTTCAACATCTGGAAGCTGTTCTAGTTTCTCTTGTTTCTTGTAATACTCCGCC
TARGET=107,36
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1319
PRIMER_LEFT_PENALTY=0.106310
PRIMER_RIGHT_PENALTY=0.025566
PRIMER_LEFT_SEQUENCE=CTCTTCCATTCCATCATCATCA
PRIMER_RIGHT_SEQUENCE=AGCTTCCAGATGTTGAAAGAGC
PRIMER_LEFT=79,22
PRIMER_RIGHT=219,22
PRIMER_LEFT_TM=59.894
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.1802
PRIMER_LEFT_1_PENALTY=0.106310
PRIMER_RIGHT_1_PENALTY=0.073900
PRIMER_LEFT_1_SEQUENCE=CTCTTCCATTCCATCATCATCA
PRIMER_RIGHT_1_SEQUENCE=GAAAGAGCTTTTGTGCACATTG
PRIMER_LEFT_1=79,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=59.894
PRIMER_RIGHT_1_TM=59.926
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=0.8218
PRIMER_LEFT_2_PENALTY=0.796207
PRIMER_RIGHT_2_PENALTY=0.025566
PRIMER_LEFT_2_SEQUENCE=CTTCCATTCCATCATCATCATC
PRIMER_RIGHT_2_SEQUENCE=AGCTTCCAGATGTTGAAAGAGC
PRIMER_LEFT_2=81,22
PRIMER_RIGHT_2=219,22
PRIMER_LEFT_2_TM=59.204
PRIMER_RIGHT_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=0.8701
PRIMER_LEFT_3_PENALTY=0.796207
PRIMER_RIGHT_3_PENALTY=0.073900
PRIMER_LEFT_3_SEQUENCE=CTTCCATTCCATCATCATCATC
PRIMER_RIGHT_3_SEQUENCE=GAAAGAGCTTTTGTGCACATTG
PRIMER_LEFT_3=81,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=59.204
PRIMER_RIGHT_3_TM=59.926
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=1.0828
PRIMER_LEFT_4_PENALTY=0.106310
PRIMER_RIGHT_4_PENALTY=0.976483
PRIMER_LEFT_4_SEQUENCE=CTCTTCCATTCCATCATCATCA
PRIMER_RIGHT_4_SEQUENCE=GATGTTGAAAGAGCTTTTGTGC
PRIMER_LEFT_4=79,22
PRIMER_RIGHT_4=211,22
PRIMER_LEFT_4_TM=59.894
PRIMER_RIGHT_4_TM=59.024
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_01731 Ps_contig_mira-and-tgicl-ass_franssen_16041
SEQUENCE=CGGTCAAAGACTAACATACTATATGACACCACGAACAAATACAAACAACATAGATACAGTTAGAGCATACACGTTTGGTCGCACATTACTTTGTGGAAGTCGACATTGTTTTGTCCGGAAGACANGCTTCTGAACCAAGCACACAACATTGGCGAAACACTTCAAGAGAAACTAGAACAGCTTCCAGATGTTGAAAGTAGCTTTTGTGCACATTGATTTTGAGTTACACTCATAGACCTGAGCATAAGATATCACTATGATGATGATGATGATGATGATGATGATGATGATGATAATGATGATGATGATGGAATGGAAGAGTAATTGTTTGTTACGTTTTTTGGTTTAGAAGTAACAAATGAACTTAGTTTAGGTTTTTTTGGTTTAGTTTATAGTTGCT
TARGET=257,36
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1216
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.106310
PRIMER_LEFT_SEQUENCE=TGGAAGTCGACATTGTTTTGTC
PRIMER_RIGHT_SEQUENCE=CTCTTCCATTCCATCATCATCA
PRIMER_LEFT=93,22
PRIMER_RIGHT=320,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.894
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=0.3887
PRIMER_LEFT_1_PENALTY=0.282356
PRIMER_RIGHT_1_PENALTY=0.106310
PRIMER_LEFT_1_SEQUENCE=GGCGAAACACTTCAAGAGAAAC
PRIMER_RIGHT_1_SEQUENCE=CTCTTCCATTCCATCATCATCA
PRIMER_LEFT_1=150,22
PRIMER_RIGHT_1=320,22
PRIMER_LEFT_1_TM=60.282
PRIMER_RIGHT_1_TM=59.894
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=171
PRIMER_PAIR_PENALTY_2=0.4118
PRIMER_LEFT_2_PENALTY=0.305456
PRIMER_RIGHT_2_PENALTY=0.106310
PRIMER_LEFT_2_SEQUENCE=ATACACGTTTGGTCGCACATTA
PRIMER_RIGHT_2_SEQUENCE=CTCTTCCATTCCATCATCATCA
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=320,22
PRIMER_LEFT_2_TM=60.305
PRIMER_RIGHT_2_TM=59.894
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=0.4297
PRIMER_LEFT_3_PENALTY=0.323355
PRIMER_RIGHT_3_PENALTY=0.106310
PRIMER_LEFT_3_SEQUENCE=CGCACATTACTTTGTGGAAGTC
PRIMER_RIGHT_3_SEQUENCE=CTCTTCCATTCCATCATCATCA
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=320,22
PRIMER_LEFT_3_TM=59.677
PRIMER_RIGHT_3_TM=59.894
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.4297
PRIMER_LEFT_4_PENALTY=0.323355
PRIMER_RIGHT_4_PENALTY=0.106310
PRIMER_LEFT_4_SEQUENCE=GTCGCACATTACTTTGTGGAAG
PRIMER_RIGHT_4_SEQUENCE=CTCTTCCATTCCATCATCATCA
PRIMER_LEFT_4=77,22
PRIMER_RIGHT_4=320,22
PRIMER_LEFT_4_TM=59.677
PRIMER_RIGHT_4_TM=59.894
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=244
=
PRIMER_SEQUENCE_ID=SSR_01732 Ps_contig_mira-and-tgicl-ass_franssen_16041
SEQUENCE=CGGTCAAAGACTAACATACTATATGACACCACGAACAAATACAAACAACATAGATACAGTTAGAGCATACACGTTTGGTCGCACATTACTTTGTGGAAGTCGACATTGTTTTGTCCGGAAGACANGCTTCTGAACCAAGCACACAACATTGGCGAAACACTTCAAGAGAAACTAGAACAGCTTCCAGATGTTGAAAGTAGCTTTTGTGCACATTGATTTTGAGTTACACTCATAGACCTGAGCATAAGATATCACTATGATGATGATGATGATGATGATGATGATGATGATGATAATGATGATGATGATGGAATGGAAGAGTAATTGTTTGTTACGTTTTTTGGTTTAGAAGTAACAAATGAACTTAGTTTAGGTTTTTTTGGTTTAGTTTATAGTTGCT
TARGET=296,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01733 Ps_contig_mira-and-tgicl-ass_franssen_16054
SEQUENCE=AGTATCKTATCAACTAGACCTTCCATTAAGGAATCAACACTCAACAACAAATGTTACATTTCATATCATATCATACATTCTGCACTTTGCATAAGTATCACACAATATTTTCCAATAAACAATNTCTAGTAGTTTTGTTTTTCCTATTTAGTCCTGCTATACAAAAAGGACACTACATATAATTGAGAATNTCTTTGAGAAACAATAAAACCAAAAAGACAAAGATTACTTACCCG
TARGET=61,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01734 Ps_contig_mira-and-tgicl-ass_franssen_16055
SEQUENCE=AGTTAAATTTCTTTATCAACTAGACCTTCCATTAAGGAATCAACACTCAACAACAATGTTACATTTCATATCATATCATACATTCTGCACTTTGCATAAGTATCACACAATATTTTCCAATAAACAACATCTAGAGTTTGTTTTTCCATTTAGTCCTGCTATCAAAAAGGACACTACATATAATTGAGAATATCTTTGAGAACATAAACCAAAAGCAAAGATATTAGGTACAAGAGGTATGCAGGAAAGGATGTCCAAAGAGGAAAAGTGGTACGAAAAGCCGAAGCTAAACCCATCACAGCACAAACAATGGCCACCACCAATACATCAGCTGAAAATTCCCAAGTAATATCTCTCATATATATAAGTATAGATATCACCACAAAACCAAGAATGTTGTTCATGAACACTGCTCCATAAATCTCATACATTGTGTGAGAAGTATTACTACTTTTCTTGTGACTAGCTTCTTTGATTGCTGATGTTGCTTCTCTGAAATTTGTAGCCAAAGGGACTAAAATAAACGAGATGAAAAACGATGAAATTCCCGCGGCTTCGGAGAATTTTTGAACACTTGCTATTAGAGGCTCGGCAAGAAGTGACAGCATAGTAATTCCCAACACCAAATATGCTATAGCTTCAAACCATGCAGAAACTCCTTTGGATTGATTCTCTTCCATTACCTTCTCAACATCTTCCCATGTTTGATGAGTTTCATGATGATGTTGAGAAGATTTTGAGGTAGAAGTTTTGTTGGAATTTGAGCTAATCCATTTGGTGAAACCATCCACAAATTCATTCTCACTGATTTCATTGTCTTTGTTGACATCAAGATCTTGAAGTAGTTTTGTGACTGCTTCTTCTGAATCTACACCCGTACCAATATGAATGTCCTTCACTATTTTCTCTAACTCAGTTCTCGAAACATGGTCATCGCCATCGATATTAACTTCCTCAAACAACCTTTTGATAGCTTCTTTATCTGGTGTACCGTCTTCATTGCGCAGCTTACCAACCATATCACTTTGAGCATGACTTAAAACTTCAGAAATAAGACGTCCCTTCAATTCGCGTTCTTTTCTCACGTTTTCAATCCACGGCTTAATAAACGTCTGATACATTTTGTTCATAGACTCTTTGGGTAGATACTTTCTATCCAAAGCATGTTTAGTTTGGTTAATGTATTCTTTTAAGCCATCAACAAATTCTTCCCTTGATATGATTTGGTCACCATTTTTGTCAAAAGCTTTTACCATGTCTTCTATTTGTTCATCTTTGATGTTCGTGTTGGTTAAATTGTTTCCGAGCAGAAGCTCTTTTACTTCAGAAGCTAATAAGTCCTTTCCCCCACGTTGACTGAATTCGTTATACAACCCGCTTATGGCAGTTACGTTCGGAGTTCCGTCCTCAGCTAAGATCTTTTGAAGAGTTTGCTTTTCAACGCGTTGGAAAATTCGCAATATTAAATCATCATGTTTTATGTATTCAAGCCTTGTTTTCTCTATCTGAGGTTTAAAGATCTGGTAAATGAAGTATGAGATGAGAAAAGAGACCGCAATGATGAGAGAAATCATCAAGGTGAAATTGCGCGACGATGTAGAGAAATGAAGTAATGAGGGAATCTGCATTATGAGGAGTGGTATAACAAGAAAAAACCATATTTCTTGCCATCTTTCTAGTGTCTACGTCCATAGTAATGCCATAACCAATACAAAGATTTGTTTTATTCTTCCTGA
TARGET=66,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1383
PRIMER_LEFT_PENALTY=2.114715
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=GACCTTCCATTAAGGAATCAACAC
PRIMER_RIGHT_SEQUENCE=CCACTTTTCCTCTTTGGACATC
PRIMER_LEFT=22,24
PRIMER_RIGHT=270,22
PRIMER_LEFT_TM=60.115
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=2.1465
PRIMER_LEFT_1_PENALTY=2.114715
PRIMER_RIGHT_1_PENALTY=0.031769
PRIMER_LEFT_1_SEQUENCE=GACCTTCCATTAAGGAATCAACAC
PRIMER_RIGHT_1_SEQUENCE=GACATCCTTTCCTGCATACCTC
PRIMER_LEFT_1=22,24
PRIMER_RIGHT_1=254,22
PRIMER_LEFT_1_TM=60.115
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=2.2451
PRIMER_LEFT_2_PENALTY=2.114715
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=GACCTTCCATTAAGGAATCAACAC
PRIMER_RIGHT_2_SEQUENCE=TCCTTTCCTGCATACCTCTTGT
PRIMER_LEFT_2=22,24
PRIMER_RIGHT_2=250,22
PRIMER_LEFT_2_TM=60.115
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=2.2540
PRIMER_LEFT_3_PENALTY=2.230415
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=CCTTCCATTAAGGAATCAACACTC
PRIMER_RIGHT_3_SEQUENCE=CCACTTTTCCTCTTTGGACATC
PRIMER_LEFT_3=24,24
PRIMER_RIGHT_3=270,22
PRIMER_LEFT_3_TM=60.230
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=2.2622
PRIMER_LEFT_4_PENALTY=2.230415
PRIMER_RIGHT_4_PENALTY=0.031769
PRIMER_LEFT_4_SEQUENCE=CCTTCCATTAAGGAATCAACACTC
PRIMER_RIGHT_4_SEQUENCE=GACATCCTTTCCTGCATACCTC
PRIMER_LEFT_4=24,24
PRIMER_RIGHT_4=254,22
PRIMER_LEFT_4_TM=60.230
PRIMER_RIGHT_4_TM=59.968
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_01735 Ps_contig_mira-and-tgicl-ass_franssen_16061
SEQUENCE=CGGGGTCCACATTCATTTTTAGCTACTTGGAAGTGTCTGTCTGTCATCCTACCATATTTAGGATGTATGCGTCAGCACTGCAGAAACCATTTTCTTTTGTGTCATTGCATGTAGTTAGTTGTGTGGATGATACGTATTGTGTCATTGAATTGATGTTGATAGTCGTATTTGTTTTGTTAAGTGTTCCA
TARGET=34,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.0531
PRIMER_LEFT_PENALTY=1.735289
PRIMER_RIGHT_PENALTY=2.317786
PRIMER_LEFT_SEQUENCE=GGGGTCCACATTCATTTTTAGC
PRIMER_RIGHT_SEQUENCE=GACACAATACGTATCATCCACACA
PRIMER_LEFT=1,22
PRIMER_RIGHT=142,24
PRIMER_LEFT_TM=61.735
PRIMER_RIGHT_TM=59.682
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=142
PRIMER_PAIR_PENALTY_1=4.0531
PRIMER_LEFT_1_PENALTY=1.735289
PRIMER_RIGHT_1_PENALTY=2.317786
PRIMER_LEFT_1_SEQUENCE=GGGGTCCACATTCATTTTTAGC
PRIMER_RIGHT_1_SEQUENCE=TGACACAATACGTATCATCCACAC
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=143,24
PRIMER_LEFT_1_TM=61.735
PRIMER_RIGHT_1_TM=59.682
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=4.5314
PRIMER_LEFT_2_PENALTY=1.735289
PRIMER_RIGHT_2_PENALTY=2.796144
PRIMER_LEFT_2_SEQUENCE=GGGGTCCACATTCATTTTTAGC
PRIMER_RIGHT_2_SEQUENCE=CACAATACGTATCATCCACACAAC
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=140,24
PRIMER_LEFT_2_TM=61.735
PRIMER_RIGHT_2_TM=59.204
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=4.6444
PRIMER_LEFT_3_PENALTY=1.735289
PRIMER_RIGHT_3_PENALTY=2.909118
PRIMER_LEFT_3_SEQUENCE=GGGGTCCACATTCATTTTTAGC
PRIMER_RIGHT_3_SEQUENCE=TCATCCACACAACTAACTACATGC
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=129,24
PRIMER_LEFT_3_TM=61.735
PRIMER_RIGHT_3_TM=59.091
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=4.7473
PRIMER_LEFT_4_PENALTY=1.735289
PRIMER_RIGHT_4_PENALTY=3.012045
PRIMER_LEFT_4_SEQUENCE=GGGGTCCACATTCATTTTTAGC
PRIMER_RIGHT_4_SEQUENCE=ACACAATACGTATCATCCACACAAC
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=141,25
PRIMER_LEFT_4_TM=61.735
PRIMER_RIGHT_4_TM=59.988
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_01736 Ps_contig_mira-and-tgicl-ass_franssen_16064
SEQUENCE=TCACTCTCATCAAACTCATCCTCAGACAAGTCAAAATTGACATGTTTCTGAGATTTGTTCGCCCCTTTCAGATCTTTTCCCCCTTTAGGATTCTGAAACTGCGCAACTTGAACACCACCACCACCACCACCACCACCACCTTTCTGACCCTTTTGATTCTGAGGCTTACTATTCTTACCTCCTTTGTTGTTTTCCATTTGCACGTTCTTAAACTGAGGTTGCTGTTGTTGCTGTTGCTGAAAGTTTTGATTTTGATTAAACACCATGCTTCTCTGGCCACCCCATAGTTCAGCATATTTCCCTGAACTTTTTCAGCTTTCTTTTATGAGCTTTGCCGGATCTACATGACCTGACAACCAAAACCTTTCCCTTGTT
TARGET=113,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0920
PRIMER_LEFT_PENALTY=0.054491
PRIMER_RIGHT_PENALTY=0.037486
PRIMER_LEFT_SEQUENCE=CTTTCAGATCTTTTCCCCCTTT
PRIMER_RIGHT_SEQUENCE=CAAAACTTTCAGCAACAGCAAC
PRIMER_LEFT=64,22
PRIMER_RIGHT=247,22
PRIMER_LEFT_TM=59.946
PRIMER_RIGHT_TM=59.963
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.1499
PRIMER_LEFT_1_PENALTY=0.054491
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=CTTTCAGATCTTTTCCCCCTTT
PRIMER_RIGHT_1_SEQUENCE=AATATGCTGAACTATGGGGTGG
PRIMER_LEFT_1=64,22
PRIMER_RIGHT_1=297,22
PRIMER_LEFT_1_TM=59.946
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.2545
PRIMER_LEFT_2_PENALTY=0.159038
PRIMER_RIGHT_2_PENALTY=0.095445
PRIMER_LEFT_2_SEQUENCE=CCCCCTTTAGGATTCTGAAACT
PRIMER_RIGHT_2_SEQUENCE=AATATGCTGAACTATGGGGTGG
PRIMER_LEFT_2=78,22
PRIMER_RIGHT_2=297,22
PRIMER_LEFT_2_TM=59.841
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.2735
PRIMER_LEFT_3_PENALTY=0.159038
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=CCCCCTTTAGGATTCTGAAACT
PRIMER_RIGHT_3_SEQUENCE=GAAAGGTTTTGGTTGTCAGGTC
PRIMER_LEFT_3=78,22
PRIMER_RIGHT_3=367,22
PRIMER_LEFT_3_TM=59.841
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.2851
PRIMER_LEFT_4_PENALTY=0.247631
PRIMER_RIGHT_4_PENALTY=0.037486
PRIMER_LEFT_4_SEQUENCE=CATGTTTCTGAGATTTGTTCGC
PRIMER_RIGHT_4_SEQUENCE=CAAAACTTTCAGCAACAGCAAC
PRIMER_LEFT_4=40,22
PRIMER_RIGHT_4=247,22
PRIMER_LEFT_4_TM=59.752
PRIMER_RIGHT_4_TM=59.963
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_01737 Ps_contig_mira-and-tgicl-ass_franssen_16067
SEQUENCE=ATATTATCTTTTGCTCTTAAGGAGAACTCATTTAAGATTTTATATATTGTAAACTAACATGCTTTCTCTGTATGGATAAATTATATGATACTTGTGTTGTGTTGTGTGGCTACTC
TARGET=92,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01738 Ps_contig_mira-and-tgicl-ass_franssen_16068
SEQUENCE=TTCAACCGAAAAAATTCAAACATTCAAACAACATCATCTCCCAAAAACCTTCAACCTAGCCAGAAACCCNCACACCCTCGCACTCATCCACGTGCATTACAAAGATTATGAAAAACTTGATCTTCATGATTTAAGCTAAGTTTATCATTGTACAATTAAAATGGAGAAACTGTTTAAAACTTGTTCTTATATTATCTTTTGCTCTTAAGGAGAACTCATTTAAGATTTTTATATATTTGTAAAACTACATGCTTTCTCTGTATGGATAAATTATATGATACTTGTGTTGTGTTGTGTGGCTACTCTGCTACATCCACTCATTGTATATTCTTTTTCTTCTTTTAAAAATAATAATATAAACTACTGTT
TARGET=282,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8522
PRIMER_LEFT_PENALTY=0.026163
PRIMER_RIGHT_PENALTY=1.826066
PRIMER_LEFT_SEQUENCE=TCTCCCAAAAACCTTCAACCTA
PRIMER_RIGHT_SEQUENCE=TGAGTGGATGTAGCAGAGTAGCC
PRIMER_LEFT=36,22
PRIMER_RIGHT=319,23
PRIMER_LEFT_TM=59.974
PRIMER_RIGHT_TM=60.826
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=52.174
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=1.9179
PRIMER_LEFT_1_PENALTY=0.026163
PRIMER_RIGHT_1_PENALTY=1.891719
PRIMER_LEFT_1_SEQUENCE=TCTCCCAAAAACCTTCAACCTA
PRIMER_RIGHT_1_SEQUENCE=GAGTGGATGTAGCAGAGTAGCC
PRIMER_LEFT_1=36,22
PRIMER_RIGHT_1=318,22
PRIMER_LEFT_1_TM=59.974
PRIMER_RIGHT_1_TM=58.108
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=1.9216
PRIMER_LEFT_2_PENALTY=0.095574
PRIMER_RIGHT_2_PENALTY=1.826066
PRIMER_LEFT_2_SEQUENCE=ACAACATCATCTCCCAAAAACC
PRIMER_RIGHT_2_SEQUENCE=TGAGTGGATGTAGCAGAGTAGCC
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=319,23
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.826
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=52.174
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=1.9370
PRIMER_LEFT_3_PENALTY=0.110942
PRIMER_RIGHT_3_PENALTY=1.826066
PRIMER_LEFT_3_SEQUENCE=CATCCACGTGCATTACAAAGAT
PRIMER_RIGHT_3_SEQUENCE=TGAGTGGATGTAGCAGAGTAGCC
PRIMER_LEFT_3=84,22
PRIMER_RIGHT_3=319,23
PRIMER_LEFT_3_TM=59.889
PRIMER_RIGHT_3_TM=60.826
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=1.9873
PRIMER_LEFT_4_PENALTY=0.095574
PRIMER_RIGHT_4_PENALTY=1.891719
PRIMER_LEFT_4_SEQUENCE=ACAACATCATCTCCCAAAAACC
PRIMER_RIGHT_4_SEQUENCE=GAGTGGATGTAGCAGAGTAGCC
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=318,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=58.108
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_01739 Ps_contig_mira-and-tgicl-ass_franssen_16070
SEQUENCE=TTTTTTTTTTAACTTTTTGTAATTGATCACAAGAAAGGAATAAAACAAAAACTACATCTCAAAGATTCAATTTCTTAGCTGTTCCTCAATAGCTTTCTCCAACCAAGTTTCAGCATCTTGCATAATTTCAGGGCTAAGCCTAACCATCAATATACAGAACTCAGTTTCATTCAAGTTTCCATCACCATCAAGATCACCTTGTTAAACCATAGCTTCTGCATCTTCTTTACTCATTCCTTCCATACCAAGCATCGCTGAGTTTTTCCTAAGACTCTCACTTGTTATCAGACCTGTTTCTTGGTCTGCTAGAAGCCTAAAACCGTTACAGAGTTCGGATACAAAGGCTTCTACATCAAGTTTCTCTGCCATCACTGGTAGTAAATCTTCGAACTCGGATTCCATGGTTGTGGGGGTTGTTTGATTCATGTTCTTGTTGGTTTCCATTGTTAAGGGAGTTTATTTTTTTGTTGTTGTTGTTGTTGTAGTTGGTAAACGTGGGAAGAAGGGTGGATGAAGAAAATGACTTTGGAAGAGATGTATATTATAAAGTAATGGGAATTTAATGAGTCCCCGAC
TARGET=465,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3479
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.252355
PRIMER_LEFT_SEQUENCE=GGGGTTGTTTGATTCATGTTCT
PRIMER_RIGHT_SEQUENCE=GGGGACTCATTAAATTCCCATT
PRIMER_LEFT=409,22
PRIMER_RIGHT=571,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=60.252
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.3947
PRIMER_LEFT_1_PENALTY=0.095574
PRIMER_RIGHT_1_PENALTY=0.299089
PRIMER_LEFT_1_SEQUENCE=GGGGTTGTTTGATTCATGTTCT
PRIMER_RIGHT_1_SEQUENCE=TCATTTTCTTCATCCACCCTTC
PRIMER_LEFT_1=409,22
PRIMER_RIGHT_1=522,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.299
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=0.4269
PRIMER_LEFT_2_PENALTY=0.174518
PRIMER_RIGHT_2_PENALTY=0.252355
PRIMER_LEFT_2_SEQUENCE=AAGTTTCTCTGCCATCACTGGT
PRIMER_RIGHT_2_SEQUENCE=GGGGACTCATTAAATTCCCATT
PRIMER_LEFT_2=354,22
PRIMER_RIGHT_2=571,22
PRIMER_LEFT_2_TM=60.175
PRIMER_RIGHT_2_TM=60.252
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=0.4552
PRIMER_LEFT_3_PENALTY=0.156135
PRIMER_RIGHT_3_PENALTY=0.299089
PRIMER_LEFT_3_SEQUENCE=ATACCAAGCATCGCTGAGTTTT
PRIMER_RIGHT_3_SEQUENCE=TCATTTTCTTCATCCACCCTTC
PRIMER_LEFT_3=241,22
PRIMER_RIGHT_3=522,22
PRIMER_LEFT_3_TM=60.156
PRIMER_RIGHT_3_TM=60.299
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=0.4620
PRIMER_LEFT_4_PENALTY=0.209637
PRIMER_RIGHT_4_PENALTY=0.252355
PRIMER_LEFT_4_SEQUENCE=TTGGTTTCCATTGTTAAGGGAG
PRIMER_RIGHT_4_SEQUENCE=GGGGACTCATTAAATTCCCATT
PRIMER_LEFT_4=433,22
PRIMER_RIGHT_4=571,22
PRIMER_LEFT_4_TM=60.210
PRIMER_RIGHT_4_TM=60.252
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_01740 Ps_contig_mira-and-tgicl-ass_franssen_16088
SEQUENCE=AACATGATATGGCAATAATCTGTCGCAGGCATCTTCTAGAGAAGAAAAGGGTCGCTTGAAATCCGGATGGCAGACCCGCCAGGCGTCTTGGTAAGCCATCTGGAGCTCGATTTGATTCCCGGGGGGTCGAATAATCTTTTGGTGCTGTTGCTGAGGTAGATGGTTTGAATTAGGGTTGTGGTGTAAATTGAGAATGGGATTAGGGTTAGGGTTAGGGTTTTGTTGAAGATTGAGGGGTCTTATGGGACGCAAGTGAGCGTCAATGTTGGAAGGGAAGCGAGAAATTGCGGATGATTGCTGTTGCTTTTGCAATTGCTGTAAGAGCAGAAAATGTTGTTGTTGTTGTTGTTGTTGTCGATGTTGTTCTTGGATCAACTGCTGCTGCTGCTGCATTCGCCYTTKCTTCTTCCATTTTTTTCTCGAAATGAAGTTATTAATCTATCTCGTCTTTCTTCCTAGGATTATTCTCAATTTTTCATCTCTCTCTGTACATATTAGTGTTTTAAACATCAGATTAGAATGGTTC
TARGET=332,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01741 Ps_contig_mira-and-tgicl-ass_franssen_16088
SEQUENCE=AACATGATATGGCAATAATCTGTCGCAGGCATCTTCTAGAGAAGAAAAGGGTCGCTTGAAATCCGGATGGCAGACCCGCCAGGCGTCTTGGTAAGCCATCTGGAGCTCGATTTGATTCCCGGGGGGTCGAATAATCTTTTGGTGCTGTTGCTGAGGTAGATGGTTTGAATTAGGGTTGTGGTGTAAATTGAGAATGGGATTAGGGTTAGGGTTAGGGTTTTGTTGAAGATTGAGGGGTCTTATGGGACGCAAGTGAGCGTCAATGTTGGAAGGGAAGCGAGAAATTGCGGATGATTGCTGTTGCTTTTGCAATTGCTGTAAGAGCAGAAAATGTTGTTGTTGTTGTTGTTGTTGTCGATGTTGTTCTTGGATCAACTGCTGCTGCTGCTGCATTCGCCYTTKCTTCTTCCATTTTTTTCTCGAAATGAAGTTATTAATCTATCTCGTCTTTCTTCCTAGGATTATTCTCAATTTTTCATCTCTCTCTGTACATATTAGTGTTTTAAACATCAGATTAGAATGGTTC
TARGET=376,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01742 Ps_contig_mira-and-tgicl-ass_franssen_16089
SEQUENCE=GAGAGAGAGATGAAAAATKGRGAATAATCCTAGGAAGAAAGACGAGATAGATTAACAATTTCGTTTCGAGAAAAAAATGGAAGAAGCAAAGGCAATGCAGCAGCAGCAGTTGATCCAAGAACAACATCGACAACAACAACAACAACAACATTTTCTGCTCTTACAGCAATTGCAAAAGCAACAGCAATCATCCGCAATTTCTCGCTTCCCTTCCAACATTGACGCTCACTTGCGTCCCA
TARGET=1,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01743 Ps_contig_mira-and-tgicl-ass_franssen_16089
SEQUENCE=GAGAGAGAGATGAAAAATKGRGAATAATCCTAGGAAGAAAGACGAGATAGATTAACAATTTCGTTTCGAGAAAAAAATGGAAGAAGCAAAGGCAATGCAGCAGCAGCAGTTGATCCAAGAACAACATCGACAACAACAACAACAACAACATTTTCTGCTCTTACAGCAATTGCAAAAGCAACAGCAATCATCCGCAATTTCTCGCTTCCCTTCCAACATTGACGCTCACTTGCGTCCCA
TARGET=97,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01744 Ps_contig_mira-and-tgicl-ass_franssen_16089
SEQUENCE=GAGAGAGAGATGAAAAATKGRGAATAATCCTAGGAAGAAAGACGAGATAGATTAACAATTTCGTTTCGAGAAAAAAATGGAAGAAGCAAAGGCAATGCAGCAGCAGCAGTTGATCCAAGAACAACATCGACAACAACAACAACAACAACATTTTCTGCTCTTACAGCAATTGCAAAAGCAACAGCAATCATCCGCAATTTCTCGCTTCCCTTCCAACATTGACGCTCACTTGCGTCCCA
TARGET=130,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01745 Ps_contig_mira-and-tgicl-ass_franssen_16091
SEQUENCE=TAAGATGTAGAAGAAGATGAGYAGGAAAAAGAAGAAGAAGAAGGTATAGATGAATATGGTGTTTGCACTCTTGGATCTTCATTGATAAAATTAGATGGATAAGGATTATGAAGAGGAGGATGAAAAGCATAAGCCATTTCGCCATGTAATCTAATTTTCTTCCAATTGAGCAACACCTAATCCTCTTTGTGGTTGCTTTGGTTTATCTTGATTATTTTTCTTCCCTTTTCTTGAAGATGATGAAGATCCACTTACTCTACCCGAC
TARGET=29,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01746 Ps_contig_mira-and-tgicl-ass_franssen_16093
SEQUENCE=ACTWAAAGTAAATTAACCAAARRACTGGTTTTATTGTGTTTTAGTCCATTCATTCCTTTCACCATGCCTTATTTATTCCAACCTCGTATAATAATAAACAAAAAGATACTGTGATCAATCTTAACTTACATAAAAAAACTACACACTAGGGAAATACACTGTCAACCATCTTCTAGGATCTCTTTGTGTACATGCATGCATGAAGTGCTGCAGTATTTGTAATTATACCGGTGGAAGGGAACTTTACCAGCAAGTGATGAATTACAACAGGAACAAACTATGTCACTGGTTAATCCACTTTTATTAGCCTCTGATATGCTATTATTAGCTTGGATTTTGAGGGCAGCCATTCTTCTCTCAGCAGCAGCTGCTCTCTTTTCTCTTTCTTCATCCTGTGCTCTTTTTAGTTCCTCCTCTTTTGATAATTTCACACCACTTCTTGGTTGTGATTGTCCTGTAGCTGATGTTGGAGCAATCTTTTGTTTTTCAGCAGCTTCAGCCTGGGCCTTCTTTTCTTTTGCCTTGCGTAATTTCTTCAATTCTTTTGCTCTAGCTTTTCTCTTGGCATCTTTTTCTGCCTGCTTAGCAGCTTGTGATTCTTCCATTTCTTTGGTCAATGCACTAGGCACTTTTGCAGCATTCCAATCCCATTTGTCAGGATTTGAAGCCATAAACCTTCTGAAAGTGTTCCTGACTTCCTTGTCAGGTGCCAACATATACGGGGTCCGTCCCCTTCCATCTTTGATGCATGGATCCAAGCCTTGTTCCAGGAGTTCCGTAACCTTTTGAGAGTCAGCAGATTGTGCTGCTTGATGTAAAGGAGTTGACTTACCAGATAACTCATTCTCACTTTCGTCGTTACTCGAAATAAGAGGTTCATCATTTTGGTTACTGGAACAAGCTTCAGCTTTGTTGTTTTTATCCAATCCAGCCATGTCCACTTTGCTGGCAGGTAGGGCACCATTTCTTTTAGCAATACGAATTGGTGGCTCCGCGTCCTGTTGATTGCTTTGTAAAACTTCCTTCTCATCTGCTTCATAGGATACTAGGGTCAACTGGCCATATACACGTTTTGCTTCCCTGAGAGTGGGCCTGCGGACAATCATAGCAATATTTCTGGCACATTGTGGATTGGTAAAACATGGCTTTTCTCCATCATAAAGCAGTTGACGACTACTTGAGGGCGCATGTATAAAAATGCAAATAGAAGCATCAAAATAAGGTCTCCAAGCAGTAAGTAGTTCGTGAACTTCCTTTTTTAATGCAAGTTCATTATAACGACGAAGTGAAGCACCAGCAGAATGTGCAGCCCTGCCAGAAGCATCATTTGTAGATTGTTTTTTCCCAGCCTTGGCCCTCACAACATATCTATGAAATGTCTTGTGAGCCACAACCGTTTCACCATCAAAGACACAGCCCGCAAAGTGCCCACCAGATGCAAGCAGAACGATCCTCAAACGGCTATTATCCCTGGGCTCAACMGTCAGAGATTTCAACCTCTCAGCCAAATTTTTCTGCACTTTGTTCATCCCCATACARCACATTTTCACTCACATTCATAATCAAACACTTCCAAACAGAAACT
TARGET=191,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01747 Ps_contig_mira-and-tgicl-ass_franssen_16122
SEQUENCE=GAGGTTATGTGAGCATTCAGTTATGTAAGTTGAGAGATTTAAGCATGGTTATTGAAGAGGATTGAGAAACAGTTAGGGAGGGTTTTGCACGGGTTGCAGAATGGAGAGGATAGAAGGTGATGAAGTGGAATGTGATTTTGAGGGTTAAGTGAGGGAATGAATGAATGATTTTATTTTTTGTAAGAAGATRGAAGGTTGAGTCTGTATTGACCATGTAAGAGCATTCACCAATGATTGGTTTGCCAATGAAATTTGCTYGGACTCTATAGTGAAGTAAAACTCTCTTTTCATGAGATTGCAATGGGTCTGTCATACCAATTA
TARGET=155,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01748 Ps_contig_mira-and-tgicl-ass_franssen_16125
SEQUENCE=TACGCGGGGGGGTTTTATAATGTTTGASATCCTCCTTTTATTTGGATAAATAAACAATGGCTACCAACGCTACCACTCTCTCTCTTTCCTTCGCCACAACCTCTTCTTCTTCCTCCTTAGTTGGTATCTCAAAACGATTCGTTTCATTCTCTACGCTGCCTTATCCTTCTCTATCGCATCCTGTTGTCACTCACAGTTCCAAACCTAGGGTTTCCACCCCCTTTGCCTTAGCGGCTGTCGTCAATGGCGACACCGCAATCGTCGATGGAGAAGAAAACGAGGAAGAAAAACCACGGAAGCTCGGAAATCCTACTCAACTTTACGTTTGCAACCTTCCTAGAAGCTGTGACATTCGACAACTAACTCACATCTTCACTCCTCACGGAACCGTTCTATCTGCACAGGTTTGTCAGAATGCTGAGACAGGTGAGAGTAAAGGAAGTGCTTATGTAACGATGGGATCTTATAATGCTGCTAAAAATGCAATTGATGCATTGGATGGATTAGGATGTTGATGKACGCGAGGTGCG
TARGET=76,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01749 Ps_contig_mira-and-tgicl-ass_franssen_16153
SEQUENCE=AAATTCGTAGAAAGAGTAATTGATCAAATTAATTAATTAAGTAAAATGTAATGAAAGGAGTAGTTTACTTGGTGTCAAATTCAGCTGCTAATTTTTCCAACTTTGGCTTCAAATATATGCACTTTCTGCACCAAGCAGCCATCCAATCAATAATAACAGGCTCAGAGTTTTGTTGAGCATGAACTAGAATTTGATCAAGCTGTTCAGAATCATTAAGAACTTCCATTTCAACCGTACTTGGTCTTGACAAATCTGGCCACATAGATTCCACACGAAATTCCTTCTTCTATCTTCCTCTCTTCTCTGTTTCTTGTTTTCTAATACAATATGTTCCCAAAACAACTCTTACGCAAACGGAGATCTTGCACTAACAAAAG
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01750 Ps_contig_mira-and-tgicl-ass_franssen_16164
SEQUENCE=CGGGATAATTGGGCAGGACAAGATTTTCCTCACTGTTGCAGAAGCTGTTGCATATTGTTCTCCAAAGCTGGATGTGAACCCCTGAAAAATTCATTGAGAAAATTGTGCTMTGTGAGAATGAAATGGATTTCAGCTTAGTAGATGGTAAGAAAAGTCAAAAGATAGAGATAACAAAAGNTGAGATATGATATGATATGATATCAACTAAACTTAAAAGG
TARGET=182,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01751 Ps_contig_mira-and-tgicl-ass_franssen_16165
SEQUENCE=TTTAACTTTTTAAAATTTTTACCCCTTAACCCTTTTTAACGGGTAATAAAGGATACCTTGTTAGCACNGTTTTCTAGTATCGGATATATCCTATATAGTCCCTTTTTACTAACTTTAGTTAGTGATATCATATCATATCATATCTCATCTTTTGTATCTCTATCTTTTGACTTTTCTACCATCTACTAAGCTGAAATCCATTTCATTCTCACATAGCAACAAATTTTACTACAAAATGAATTTTTCAGGGGTTCACATCCAGCTTTGGAGAACAATATGCAACAGCTTCTGCAACAGTGAGGAAAATCTTGTCCTGCCCAAT
TARGET=125,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4540
PRIMER_LEFT_PENALTY=1.449676
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=TACCCCTTAACCCTTTTTAACG
PRIMER_RIGHT_SEQUENCE=ATTGTTCTCCAAAGCTGGATGT
PRIMER_LEFT=19,22
PRIMER_RIGHT=275,22
PRIMER_LEFT_TM=58.550
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.7000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=1.5878
PRIMER_LEFT_1_PENALTY=1.449676
PRIMER_RIGHT_1_PENALTY=0.138114
PRIMER_LEFT_1_SEQUENCE=TACCCCTTAACCCTTTTTAACG
PRIMER_RIGHT_1_SEQUENCE=GCTGTTGCATATTGTTCTCCAA
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=285,22
PRIMER_LEFT_1_TM=58.550
PRIMER_RIGHT_1_TM=60.138
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=1.6751
PRIMER_LEFT_2_PENALTY=1.449676
PRIMER_RIGHT_2_PENALTY=0.225403
PRIMER_LEFT_2_SEQUENCE=TACCCCTTAACCCTTTTTAACG
PRIMER_RIGHT_2_SEQUENCE=AGCTGTTGCATATTGTTCTCCA
PRIMER_LEFT_2=19,22
PRIMER_RIGHT_2=286,22
PRIMER_LEFT_2_TM=58.550
PRIMER_RIGHT_2_TM=59.775
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=1.6927
PRIMER_LEFT_3_PENALTY=1.449676
PRIMER_RIGHT_3_PENALTY=0.243031
PRIMER_LEFT_3_SEQUENCE=TACCCCTTAACCCTTTTTAACG
PRIMER_RIGHT_3_SEQUENCE=GCAGGACAAGATTTTCCTCACT
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=316,22
PRIMER_LEFT_3_TM=58.550
PRIMER_RIGHT_3_TM=59.757
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=1.7140
PRIMER_LEFT_4_PENALTY=1.449676
PRIMER_RIGHT_4_PENALTY=0.264300
PRIMER_LEFT_4_SEQUENCE=TACCCCTTAACCCTTTTTAACG
PRIMER_RIGHT_4_SEQUENCE=TGCATATTGTTCTCCAAAGCTG
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=280,22
PRIMER_LEFT_4_TM=58.550
PRIMER_RIGHT_4_TM=60.264
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_01752 Ps_contig_mira-and-tgicl-ass_franssen_16167
SEQUENCE=AACCAAAGACAATTCANCCTTTGATTCCCNCCCAAAGTTCCAATATCTGTTTATATCTTCTTCATCTTTCCAACCAATCTTCTCTTAACTCTTATGATTAAGATGAAGACTCGTGCCTCTACCAAAATCTCTGCTAAATGGATTCCAATTTTCTCTGTCTTTTCCTTCCTTATTGGCATGCTTATCACAAACAGGATGTGGGAGCAACCTGAATCAAATGGTTTGATAATTTCTAAGCATCAGCGTGATCAGCAAGAACTTCAACTGATCTCAGAAGATTGTTCTAACAAAAAGAAGCAGGAAAAGCCTAAGGATGAAATGAATGAATTGTATAAAACCCATGAAGCTATTCAGGCTCTAGACAAACAAGTTTCTATGTTGCAAATGGAATTAGCAGCGGCTAGGAGTTCTCGCGAAAAGAACTCCACTGGCTCGGCCACCGGTTCTGCGGAAGGTGCTTCCAAGAAGAAGAAGGCATTTGTAGTGATAGGTATAAACACGGCTTTCAGTAGTAGGAAGCGGCGTGATTCGGTTAGAGAAACTTGGATGCCGCAAGGCGAGCAACTTCTTCAATTGGAACGAGAGAAAGGAATCGTCATCAGATTCATGATTGGCCACAGTGCAACTTCAAACAGCATTCTAGATCGAGCTATTGATTCAGAAGAAGCTCAACACAAAGACTTTCTTCGCCTGCAACACGTGGAAGGGTATCATGAGCTGTCTGCAAAGACAAAGWCTTTCTTTTCACTGCTGTTGCAATATGGGATGCTGATTTCTATGTACAAAGTGGATGATGATGTTCATGTCTAAG
TARGET=463,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01753 Ps_contig_mira-and-tgicl-ass_franssen_16182
SEQUENCE=GTCTGTGTGTGAGAGAGAGAACTTGGGGCAGATGATTTACCAGTTTAAGTACTAGTATGTTTTACTTGTCCTTGAGAGACCTTCAGTTTAAGTATATCCACGTAGTATTGACTGTGTTTACCTGCTCAAAATGTTTTTATGTAAAATGATTTACTAGTTTAAGTGTTTTATTTAAAATGATTTACCAGTTTAAATAAAACACTTGAAACGAGATGAAAGGAAGAACCCTAGACAAATTAATTATAGT
TARGET=11,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01754 Ps_contig_mira-and-tgicl-ass_franssen_16218
SEQUENCE=CCACCATCACCGCCACCACCACCATGGCCCTTCTTCTTATTAGCCACAGGTGAAAAAATCCCAGCACTATGAGGAGTAGGGTACCCATTTCCACCATTTACATTATTAACACCACCATTGACTCTACCAACACCATTTTCCTCATA
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01755 Ps_contig_mira-and-tgicl-ass_franssen_16219
SEQUENCE=ATAGTGCTGGGATTTTTTCTACCTGTGGCTAATAAGAAGAAGGGCCATGGTGGTGGTGGTGGTGATGGTGGAAAAGATCTTCATATGTTTGTTTGGAGTTCAAGTGCTTCACCTGTTTCTGAAGGTGGAATTCATGTCTTTAGAGGTGCTGGAGAGTATGGGAATGAACATCTTAATGGGGTAGCTCACCAAAAAGA
TARGET=48,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2659
PRIMER_LEFT_PENALTY=0.242357
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=TGTGGCTAATAAGAAGAAGGGC
PRIMER_RIGHT_SEQUENCE=GAATTCCACCTTCAGAAACAGG
PRIMER_LEFT=23,22
PRIMER_RIGHT=132,22
PRIMER_LEFT_TM=59.758
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=0.2741
PRIMER_LEFT_1_PENALTY=0.242357
PRIMER_RIGHT_1_PENALTY=0.031769
PRIMER_LEFT_1_SEQUENCE=TGTGGCTAATAAGAAGAAGGGC
PRIMER_RIGHT_1_SEQUENCE=GTTCATTCCCATACTCTCCAGC
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=168,22
PRIMER_LEFT_1_TM=59.758
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.3670
PRIMER_LEFT_2_PENALTY=0.242357
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=TGTGGCTAATAAGAAGAAGGGC
PRIMER_RIGHT_2_SEQUENCE=TCCAGCACCTCTAAAGACATGA
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=152,22
PRIMER_LEFT_2_TM=59.758
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=130
PRIMER_PAIR_PENALTY_3=0.3859
PRIMER_LEFT_3_PENALTY=0.242357
PRIMER_RIGHT_3_PENALTY=0.143555
PRIMER_LEFT_3_SEQUENCE=TGTGGCTAATAAGAAGAAGGGC
PRIMER_RIGHT_3_SEQUENCE=TGGTGAGCTACCCCATTAAGAT
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=190,22
PRIMER_LEFT_3_TM=59.758
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=0.3859
PRIMER_LEFT_4_PENALTY=0.242357
PRIMER_RIGHT_4_PENALTY=0.143555
PRIMER_LEFT_4_SEQUENCE=TGTGGCTAATAAGAAGAAGGGC
PRIMER_RIGHT_4_SEQUENCE=GGTGAGCTACCCCATTAAGATG
PRIMER_LEFT_4=23,22
PRIMER_RIGHT_4=189,22
PRIMER_LEFT_4_TM=59.758
PRIMER_RIGHT_4_TM=59.856
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_01756 Ps_contig_mira-and-tgicl-ass_franssen_16228
SEQUENCE=TGTTACTTGAACTGCAAGTAATGGGTGTGATGTTCCTTGGTAGTTTTTGACTCCGTTCATTAAAAAAAGTGAGTGATGAACATAAGGAAGATGAGTGGGTTTAAGAATATCATCCACAGTAATATATTTTGCTGCAGCATGAACAAAGAGTGCACCTTTGTTATTGCACTTGATAGAACAAGAGATAGTGTTATCTCCGTGTTGTGTCATTTTGAGACGACCCGCGAAGATGTGGAAAAAATCAATGGCAGAGGAAAAGGATTGTTTAAGGTGTTGGATTTGGTTTAATATATCTGATTTGATTGATTGACGATAAACAAAACTTATTTGATTATATTCAAATGGGAGGAATTGTAGATCCCATGGGGATAGATAGATCATACTATCATTTGAGCCATTGAGATTGTGTATTTTGTAGAAACGTAAATTTATTGGTGGGTAAGGG
TARGET=299,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4870
PRIMER_LEFT_PENALTY=0.102129
PRIMER_RIGHT_PENALTY=0.384836
PRIMER_LEFT_SEQUENCE=ATCTCCGTGTTGTGTCATTTTG
PRIMER_RIGHT_SEQUENCE=GGGATCTACAATTCCTCCCATT
PRIMER_LEFT=192,22
PRIMER_RIGHT=361,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=60.385
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.5577
PRIMER_LEFT_1_PENALTY=0.172839
PRIMER_RIGHT_1_PENALTY=0.384836
PRIMER_LEFT_1_SEQUENCE=GCACCTTTGTTATTGCACTTGA
PRIMER_RIGHT_1_SEQUENCE=GGGATCTACAATTCCTCCCATT
PRIMER_LEFT_1=151,22
PRIMER_RIGHT_1=361,22
PRIMER_LEFT_1_TM=60.173
PRIMER_RIGHT_1_TM=60.385
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.6245
PRIMER_LEFT_2_PENALTY=0.102129
PRIMER_RIGHT_2_PENALTY=0.522348
PRIMER_LEFT_2_SEQUENCE=ATCTCCGTGTTGTGTCATTTTG
PRIMER_RIGHT_2_SEQUENCE=TCTATCCCCATGGGATCTACAA
PRIMER_LEFT_2=192,22
PRIMER_RIGHT_2=372,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.522
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=181
PRIMER_PAIR_PENALTY_3=0.6952
PRIMER_LEFT_3_PENALTY=0.172839
PRIMER_RIGHT_3_PENALTY=0.522348
PRIMER_LEFT_3_SEQUENCE=GCACCTTTGTTATTGCACTTGA
PRIMER_RIGHT_3_SEQUENCE=TCTATCCCCATGGGATCTACAA
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=372,22
PRIMER_LEFT_3_TM=60.173
PRIMER_RIGHT_3_TM=60.522
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.7026
PRIMER_LEFT_4_PENALTY=0.317786
PRIMER_RIGHT_4_PENALTY=0.384836
PRIMER_LEFT_4_SEQUENCE=GAGTGCACCTTTGTTATTGCAC
PRIMER_RIGHT_4_SEQUENCE=GGGATCTACAATTCCTCCCATT
PRIMER_LEFT_4=147,22
PRIMER_RIGHT_4=361,22
PRIMER_LEFT_4_TM=59.682
PRIMER_RIGHT_4_TM=60.385
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_01757 Ps_contig_mira-and-tgicl-ass_franssen_16235
SEQUENCE=ACTCAAATCTGAATGAAACTATACTTTTAATGTAATTTTGAGCATTTTTGCTTTTAAAGAAAGTGCTATTTAGTCAGATCTATTTTTGCCAAAGCTTTTTTTTGACAAAGTTTCTGGTTATTTTTTCCTTGACTAGATAGCATTACTTATATTATTTTTATTATTATTATTGTTTGATACTTATGGTTAAGATATATATGTTATGGAATTCTTTTTGGTACTTTATTTGTTATACTATAGATCTCTAACTATTTGTTAAA
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01758 Ps_contig_mira-and-tgicl-ass_franssen_16254
SEQUENCE=AGTTTGGTAATCATTGTTGCAAACATTTCATTGATTTTTACACGCTGTTTTAAGTATCTCGATTTAGTCAAATGATAAATCACCTCAAGTTAAGCAATAAAAATGATAAGGGAAAACCTATGCTTGAGATTGATTACATTCTTCGCCATGTTTTGCTGGTCAACTATCATGCCAGAAATCGGTATCCTGCTGGAGTCTTTTGAAGTTTATGAACTAAAAATCCATCAAGCCCTTTTGGCAAAATTATCAGTGTCATCATCATTGTCATCCCTAAAATTTCCCAAATCCTCATTTACATCTCCACTGTCTTTGCTATCAAAGTGTCCACCAGCAGAGCCAGTGCCACTGCCTGCACCATCAACGTCAGCGGGAAAACTACTGCTGCCATATCCTAACCCCCCACTTCCATCATATCCACCAGCAGAGCTAGTGCCACTACCTGCACCACCAAAGTCAGCCGGAAAACCACCGCTGCCATCATTCCCATAACTCGTCCCACTTCCACTGGGAGCAGAATAGTTATTGCCAGCATCACTGCCACCATAATTCCCACCTGGCCCAGAGCTGCCTCCACCATATCCACCACCACCGTAACCTCCACCATAGCCACCACCACCACCTGCATACCCAGCTCCACCATAGCCCCCACCACCACCTGCATACCCAGCTCCACCACCATATGGTGCATTGCCATAAGAACCACCGCCACCACCATAAACACGAGGCCTTTCATTAGCATAATTTACCCTAACTCGACGACCATGAAGGTCCTGACCATCCAAGGCCTGAATAGCACTAGATGCCTCCTCAACTGAATTGAATGTGATAAAGCCAAATCCTCTGGACCTACCAGTTTCACGATCCATAATCACCCTAGCATCCAGTACTTCGCCGTATCTTGAAAAAGCCTCCCTCAAACTTGGCTCATCAGTTGAATACGAAATACCCCCTATAAATAGCTTTGTGGTTGGAGCAGAACACATGCATCTTATAGCCTGGAAAGCAGATGGAGAAGGACGCAATTCTGAAATAATCTTCCTGTTTGTAGCTTGTCGTAGAATACTCCCAAGTTTGCCAAAGAAAGCCATGGTTGTGACTAGTAACCTGCGTTCTAGCTTGAGCTTGTCTGAGTCTAAAACCCTAAGCAAGATATTTGTCYCCGA
TARGET=607,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01759 Ps_contig_mira-and-tgicl-ass_franssen_16277
SEQUENCE=CTAAGAGGAAAGCTAGTGGAATTCTTGAGAGGTTGCATAGGGTTGTTAATATCATACACACTGCATAATAATGTTCCTTGATTCTTCTCTCTCTCTCTCTCAAGGTATATTGTTCATATGTGAGCCTTTAAGTTATTGAAGGTAAAAATCTATAAGCACTTATGTTTCAAAAACAATTATGTACATTCTTTTCTCTCAATGAAGTTATTCTCATGTTAATTAGCCAGTTACAGAATAAAGTGAAGCTATATATATATATGCTCTTTGTCGATGAATTGTTAAAGAATTTCTACAAA
TARGET=86,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.5349
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=3.404543
PRIMER_LEFT_SEQUENCE=TCTTGAGAGGTTGCATAGGGTT
PRIMER_RIGHT_SEQUENCE=GCTTCACTTTATTCTGTAACTGGC
PRIMER_LEFT=22,22
PRIMER_RIGHT=245,24
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=58.595
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=3.5349
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=3.404543
PRIMER_LEFT_1_SEQUENCE=TTCTTGAGAGGTTGCATAGGGT
PRIMER_RIGHT_1_SEQUENCE=GCTTCACTTTATTCTGTAACTGGC
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=245,24
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=58.595
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=3.6476
PRIMER_LEFT_2_PENALTY=0.243031
PRIMER_RIGHT_2_PENALTY=3.404543
PRIMER_LEFT_2_SEQUENCE=AGTGGAATTCTTGAGAGGTTGC
PRIMER_RIGHT_2_SEQUENCE=GCTTCACTTTATTCTGTAACTGGC
PRIMER_LEFT_2=14,22
PRIMER_RIGHT_2=245,24
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=58.595
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=3.6789
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=3.548548
PRIMER_LEFT_3_SEQUENCE=TCTTGAGAGGTTGCATAGGGTT
PRIMER_RIGHT_3_SEQUENCE=GCTTCACTTTATTCTGTAACTGGCT
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=245,25
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=59.451
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=3.6789
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=3.548548
PRIMER_LEFT_4_SEQUENCE=TTCTTGAGAGGTTGCATAGGGT
PRIMER_RIGHT_4_SEQUENCE=GCTTCACTTTATTCTGTAACTGGCT
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=245,25
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.451
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_01760 Ps_contig_mira-and-tgicl-ass_franssen_16277
SEQUENCE=CTAAGAGGAAAGCTAGTGGAATTCTTGAGAGGTTGCATAGGGTTGTTAATATCATACACACTGCATAATAATGTTCCTTGATTCTTCTCTCTCTCTCTCTCAAGGTATATTGTTCATATGTGAGCCTTTAAGTTATTGAAGGTAAAAATCTATAAGCACTTATGTTTCAAAAACAATTATGTACATTCTTTTCTCTCAATGAAGTTATTCTCATGTTAATTAGCCAGTTACAGAATAAAGTGAAGCTATATATATATATGCTCTTTGTCGATGAATTGTTAAAGAATTTCTACAAA
TARGET=247,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01761 Ps_contig_mira-and-tgicl-ass_franssen_16280
SEQUENCE=TAAAAAGACTTACAACAAAGTAGACAAAAGACWTTAYTCATATTACCTATCTTGTCATTGTCAAATTTCTACAACAAAGAACATTCAAGGGGAAAACGTAGGAAAAAACCTAAACTAGGGGGAAAARTTGCTCTCACAATCAGGTGGCTTTTGCATTCCCCTTGGTCTCCCCGGAACAACAACCACCGCCATGGTGGTGGTGGTGGTGGTGATGCTGATGCTGGTGCTGAGGCAACTGCTTCCCCTTGAGTTGCTTCCTCATGTCATAAGCATCCTCCCCATCAGCATAATACTTGGCCTCCACATCATGAATCTTATACCCTAAGGTCTCCGTGTACAAGTTAAAAGCTGCGCGGTTGCTCTTCCTGACGTGCAGTGAGACATACTCAGCACCAAACACCTGTTCCATGGCGTTTTGTGCGGCGGTCATTAGCTTAGTAGCAATACCAAGTTTCCGATGAGTTCTCAGAACCGCAAGAGAGGTAATATGTCCATGACACTCATTAGTCTCCTCTTCCATCTTCGCGAGGACGTAACCGACGATACGGCCGTTGTAATCTTCGGCGACGTAAAGAAGCTGCGGCCAAGAGAGGATGTGGTAAAGGTAATACTTCATCTGGTAATTCTCAGGGAGACAGAAGAGATTGCAAGCTTGCATCGCGAGGAGGTCGTTGACCGTTGCTTTTCTTATGCACACCATGGTTGGGGATTTTTGATTTTGGGGGTTCGGGTTAGGGTTCCGATTGAAGGGGTTTGGGAGGGAAGGGAATGGTGAAGAGAGAATCTGCTACCCCYGAC
TARGET=192,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01762 Ps_contig_mira-and-tgicl-ass_franssen_16284
SEQUENCE=GATAATGGAATAATAATAATCACTTAAAATTTTTCACCCAACAAATCCCTTACCCAAATTCAAATCCCCTCCTCCGTCCATGCGAACTGCATGGCTCCACCGTCCGAACCTCATCGCCGCCGTCACAACAACCACAATCACAACCACATCTTCTCCTTCTTCCAATCCACCGCTTCCAATTTCCTCTCTCTCTTCAACCATCACAATCCTCCACCTTCACCTCTACCTCAACTACCCTCCGCAGTCTCCCTCCCCCTCTTCTTCGCGCCACCTCTCTCCGCTGTGACGTTCGAAACCACCTCCGACTCAACTCGCTCTTCCGCTAAATCGGTTAGAGTGTCGAGGTTCAATTCCAATGTCAAAGGTGGTGGTGGTCCCGCTTTTGTTGGTCAGGTTTTCAGCATGTGTGACCTCTCTGGAACTGGTCTCATGGCTGTTTCTACTCACTTTGATATTCCTTTCATCACAACAAGAACCCCAGAGTGGCTGAAAAAAATGTTTGCAGCAATCACCAAGAGTGAGAGGAATGGCCCTGTATTTCGCTTTTTTATTGATCTAAGAGATGCAGTTTCATATGTCAAAAAACTCGATATTCCAAGTGGCGTGGTGGGTGCCTGCCGTCTTGATTTGGCATATAAGCATTTCAAGGAAAAGCCTCACTTATTCCAATTTGTTCCAAACGAGAAGCAGGTCAAGGCAGCTAATAAACTTCTGAAGACCATCCCACACAATATCGCTGGGAAAAAGGTCGATGGGGTGCCTGTGTTTAGTGCTCAGAACTTGGATATTGCAATAGCAACTACCGATGGAATTAAATGGTATACCCCCTACTTCTTTGATAAAAACATGCTTGATAACATTCTTGAAGAAGCTGTTGATCAACATTTCCATACATTAATCCAAACTCGGCACATTCAGCGGCGACGAGATGTGGTTGATGACAACTTGGCAGCAGAAGTGATTGAGGAAATTGGAGATAGTTTAGGGGAGCCTCCAGAGGTTCAAGAAGTGCTTGATGAAATGGGCCATCCTAGTATACCACTAAGTGTTATTTCTAAAGCTGCAGAACTCCAGTTCCATTATACTGTTGACAAAGTGCTCTTGGGAAATAGGTGGTTGCGGAAAGCAACAGGCATACAACCGAACTTTCCATATATGGTTGACTCATTTGAAAGAAGAAGTGAAGCCTCTTTTCTGAGAATTACAGAGTCAAGCAGCTGTCCCGATAACTCTAAATTGGAAGATGATGCTAAACAAACTGGGTGTACAGACTCTTCTGAATATAGTTTGGATGACAGCACTGAAAAAGTAGGGTTATCAAGAAAAGGTGTAAATGAAGAATATTTGAAACAAAATACCTTTCTTCCAAAAATTACAATGGTTGGTCTCTCTACAGAAGAAGCAGGCCAGATGGGCAAAGCTAGTTTGAAAAAAACAATGGATGATTTGACAAAAGAGTTGGAGGAGACGGAACTAGATAATATAACTGATGGTGGTAGTAATGAGTACAAATTAGAAGATGATAGGGATCCACTATTTGTAGCCAATGTGGGTGATTATTATTCTAGTATTGGAAGGACACGAACTCCTCGTTGGATTCGAGGAGGGTCAAACTAAAACTTGCACGGTGACTGCTTCTCATTTTGATGGAAAACTCGGGAACTACACACCGGATTTGAGGAGGGTCCAATTAAAACCTTTTTGCTATCAAGAAGAATGTTTCATTTGGGGGTAGTTTTGGATATTTATTGGTGTCTCCARACAAAAAAAATCNGGTTACTGTACTAA
TARGET=9,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01763 Ps_contig_mira-and-tgicl-ass_franssen_16284
SEQUENCE=GATAATGGAATAATAATAATCACTTAAAATTTTTCACCCAACAAATCCCTTACCCAAATTCAAATCCCCTCCTCCGTCCATGCGAACTGCATGGCTCCACCGTCCGAACCTCATCGCCGCCGTCACAACAACCACAATCACAACCACATCTTCTCCTTCTTCCAATCCACCGCTTCCAATTTCCTCTCTCTCTTCAACCATCACAATCCTCCACCTTCACCTCTACCTCAACTACCCTCCGCAGTCTCCCTCCCCCTCTTCTTCGCGCCACCTCTCTCCGCTGTGACGTTCGAAACCACCTCCGACTCAACTCGCTCTTCCGCTAAATCGGTTAGAGTGTCGAGGTTCAATTCCAATGTCAAAGGTGGTGGTGGTCCCGCTTTTGTTGGTCAGGTTTTCAGCATGTGTGACCTCTCTGGAACTGGTCTCATGGCTGTTTCTACTCACTTTGATATTCCTTTCATCACAACAAGAACCCCAGAGTGGCTGAAAAAAATGTTTGCAGCAATCACCAAGAGTGAGAGGAATGGCCCTGTATTTCGCTTTTTTATTGATCTAAGAGATGCAGTTTCATATGTCAAAAAACTCGATATTCCAAGTGGCGTGGTGGGTGCCTGCCGTCTTGATTTGGCATATAAGCATTTCAAGGAAAAGCCTCACTTATTCCAATTTGTTCCAAACGAGAAGCAGGTCAAGGCAGCTAATAAACTTCTGAAGACCATCCCACACAATATCGCTGGGAAAAAGGTCGATGGGGTGCCTGTGTTTAGTGCTCAGAACTTGGATATTGCAATAGCAACTACCGATGGAATTAAATGGTATACCCCCTACTTCTTTGATAAAAACATGCTTGATAACATTCTTGAAGAAGCTGTTGATCAACATTTCCATACATTAATCCAAACTCGGCACATTCAGCGGCGACGAGATGTGGTTGATGACAACTTGGCAGCAGAAGTGATTGAGGAAATTGGAGATAGTTTAGGGGAGCCTCCAGAGGTTCAAGAAGTGCTTGATGAAATGGGCCATCCTAGTATACCACTAAGTGTTATTTCTAAAGCTGCAGAACTCCAGTTCCATTATACTGTTGACAAAGTGCTCTTGGGAAATAGGTGGTTGCGGAAAGCAACAGGCATACAACCGAACTTTCCATATATGGTTGACTCATTTGAAAGAAGAAGTGAAGCCTCTTTTCTGAGAATTACAGAGTCAAGCAGCTGTCCCGATAACTCTAAATTGGAAGATGATGCTAAACAAACTGGGTGTACAGACTCTTCTGAATATAGTTTGGATGACAGCACTGAAAAAGTAGGGTTATCAAGAAAAGGTGTAAATGAAGAATATTTGAAACAAAATACCTTTCTTCCAAAAATTACAATGGTTGGTCTCTCTACAGAAGAAGCAGGCCAGATGGGCAAAGCTAGTTTGAAAAAAACAATGGATGATTTGACAAAAGAGTTGGAGGAGACGGAACTAGATAATATAACTGATGGTGGTAGTAATGAGTACAAATTAGAAGATGATAGGGATCCACTATTTGTAGCCAATGTGGGTGATTATTATTCTAGTATTGGAAGGACACGAACTCCTCGTTGGATTCGAGGAGGGTCAAACTAAAACTTGCACGGTGACTGCTTCTCATTTTGATGGAAAACTCGGGAACTACACACCGGATTTGAGGAGGGTCCAATTAAAACCTTTTTGCTATCAAGAAGAATGTTTCATTTGGGGGTAGTTTTGGATATTTATTGGTGTCTCCARACAAAAAAAATCNGGTTACTGTACTAA
TARGET=184,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01764 Ps_contig_mira-and-tgicl-ass_franssen_16284
SEQUENCE=GATAATGGAATAATAATAATCACTTAAAATTTTTCACCCAACAAATCCCTTACCCAAATTCAAATCCCCTCCTCCGTCCATGCGAACTGCATGGCTCCACCGTCCGAACCTCATCGCCGCCGTCACAACAACCACAATCACAACCACATCTTCTCCTTCTTCCAATCCACCGCTTCCAATTTCCTCTCTCTCTTCAACCATCACAATCCTCCACCTTCACCTCTACCTCAACTACCCTCCGCAGTCTCCCTCCCCCTCTTCTTCGCGCCACCTCTCTCCGCTGTGACGTTCGAAACCACCTCCGACTCAACTCGCTCTTCCGCTAAATCGGTTAGAGTGTCGAGGTTCAATTCCAATGTCAAAGGTGGTGGTGGTCCCGCTTTTGTTGGTCAGGTTTTCAGCATGTGTGACCTCTCTGGAACTGGTCTCATGGCTGTTTCTACTCACTTTGATATTCCTTTCATCACAACAAGAACCCCAGAGTGGCTGAAAAAAATGTTTGCAGCAATCACCAAGAGTGAGAGGAATGGCCCTGTATTTCGCTTTTTTATTGATCTAAGAGATGCAGTTTCATATGTCAAAAAACTCGATATTCCAAGTGGCGTGGTGGGTGCCTGCCGTCTTGATTTGGCATATAAGCATTTCAAGGAAAAGCCTCACTTATTCCAATTTGTTCCAAACGAGAAGCAGGTCAAGGCAGCTAATAAACTTCTGAAGACCATCCCACACAATATCGCTGGGAAAAAGGTCGATGGGGTGCCTGTGTTTAGTGCTCAGAACTTGGATATTGCAATAGCAACTACCGATGGAATTAAATGGTATACCCCCTACTTCTTTGATAAAAACATGCTTGATAACATTCTTGAAGAAGCTGTTGATCAACATTTCCATACATTAATCCAAACTCGGCACATTCAGCGGCGACGAGATGTGGTTGATGACAACTTGGCAGCAGAAGTGATTGAGGAAATTGGAGATAGTTTAGGGGAGCCTCCAGAGGTTCAAGAAGTGCTTGATGAAATGGGCCATCCTAGTATACCACTAAGTGTTATTTCTAAAGCTGCAGAACTCCAGTTCCATTATACTGTTGACAAAGTGCTCTTGGGAAATAGGTGGTTGCGGAAAGCAACAGGCATACAACCGAACTTTCCATATATGGTTGACTCATTTGAAAGAAGAAGTGAAGCCTCTTTTCTGAGAATTACAGAGTCAAGCAGCTGTCCCGATAACTCTAAATTGGAAGATGATGCTAAACAAACTGGGTGTACAGACTCTTCTGAATATAGTTTGGATGACAGCACTGAAAAAGTAGGGTTATCAAGAAAAGGTGTAAATGAAGAATATTTGAAACAAAATACCTTTCTTCCAAAAATTACAATGGTTGGTCTCTCTACAGAAGAAGCAGGCCAGATGGGCAAAGCTAGTTTGAAAAAAACAATGGATGATTTGACAAAAGAGTTGGAGGAGACGGAACTAGATAATATAACTGATGGTGGTAGTAATGAGTACAAATTAGAAGATGATAGGGATCCACTATTTGTAGCCAATGTGGGTGATTATTATTCTAGTATTGGAAGGACACGAACTCCTCGTTGGATTCGAGGAGGGTCAAACTAAAACTTGCACGGTGACTGCTTCTCATTTTGATGGAAAACTCGGGAACTACACACCGGATTTGAGGAGGGTCCAATTAAAACCTTTTTGCTATCAAGAAGAATGTTTCATTTGGGGGTAGTTTTGGATATTTATTGGTGTCTCCARACAAAAAAAATCNGGTTACTGTACTAA
TARGET=364,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01765 Ps_contig_mira-and-tgicl-ass_franssen_16326
SEQUENCE=GTKACAGACTAGTCTCCATCAACTAGGTGAAAGCTCGCACATTCATTCATTTTTACTTCCTACGTTRCTGTTTCGCACTTCAAATTTGTGAGTAAATCATTCATCACTCTCATCACTACTACTACTACTACAATGTTTCATTTTCTAATTCCCAGATTCATTCCTCATACGACACTTCAATGTCCCTATTGTTTTTTCTACGTTTTATTCTAATTTTCGTTTCTTTCTTCCGTTTTCAGTAA
TARGET=40,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01766 Ps_contig_mira-and-tgicl-ass_franssen_16326
SEQUENCE=GTKACAGACTAGTCTCCATCAACTAGGTGAAAGCTCGCACATTCATTCATTTTTACTTCCTACGTTRCTGTTTCGCACTTCAAATTTGTGAGTAAATCATTCATCACTCTCATCACTACTACTACTACTACAATGTTTCATTTTCTAATTCCCAGATTCATTCCTCATACGACACTTCAATGTCCCTATTGTTTTTTCTACGTTTTATTCTAATTTTCGTTTCTTTCTTCCGTTTTCAGTAA
TARGET=115,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01767 Ps_contig_mira-and-tgicl-ass_franssen_16331
SEQUENCE=CAATGCAACAGCAAGCAAATCCATTTGGTCCCTATCAACAGTTTCCGCCTCAGCAGCAGCAGCAGCATATGTTAATGGACCCAGCAAATCCCTTTGCTGATTCAGGATTTGGGGCTTTTCATGCAAATCCCGTTTCTCACCCCCAGAATAACAATCCATTTGGAAGCACAGGCTTGTTGTAAGAAATAAGGATCGYCCCTTTTGGAGACGTCGTGTATTTAATCAATCATGTTGGTTTTTKTACWWTTAGAAGCG
TARGET=51,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01768 Ps_contig_mira-and-tgicl-ass_franssen_16332
SEQUENCE=CCAAATCCATTTGAAGTTCAYGATCCGTTTGCWGTATCAAGCAGTGTCGCCCCCCCAACCGCTGTCCAAATGGCAGCAATGCAACAGCAAGCAAATCCATTTGGTCCCTATCAACAGTTTCCGCCTCAGCAGCAGCAGCAGCATATGTTAATGGACCCAGCAAATCCCTTTGCTGATTCAGGATTTGGGGCTTTTCATGCAAATCCCGTTTCTCACCCCCAGAATAACAATCCATTTGGAAGCACAGGCTTGTTGTAAGAAATAAGGATGCCCCTTTTTGGAGACGTCGTGTATTTAATCAATCATGTTGGTTTTTTTGTACATTTAGAAGCGAGTAAATTGTTTGTTACACGAGTTTACTCATCTGTTGCTAGATACTCATCTTCTGGAATGGAAGGTGCCTTGTAAAATCAAAGATGTATTTCATCTTTTATGATATATGCATAATGGTGGGATCGCTTTCTTTAAATATCATTATTTTATGATTTAATACATGTTTATATTATGYG
TARGET=127,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01769 Ps_contig_mira-and-tgicl-ass_franssen_16350
SEQUENCE=AACAAGAACCCTAATTCTAAAATTAAATATCGATGGTGAGTCTAATTTGGAACATGTTAGCATGGGGGAAACACTCAGCACAAGAAAAGCAACAAGATCGTATCAAGAGATTAGAGAAAAGTGAAGAAAATACCTAATTAGAAACTGGAGATTTCCAGATCCCAGTTGAAGTTCACGGCGAGTATGCACTTTCCGGCGAGAATTTCTAGAAACCGACAAAGTCCAAATTTCTCATGTAACTTTTTTTTCTTGCTCTTTCTCCTTGTATCCTTCTTCTATTTCTTCTCTTAATCTCCTTCCACCTTAAATCTCACTCACTTGATTCCCCAAACACCTCTAAATTCTTTGCTCTGTTTTGGAAACTAGTTTAATGAGGGAAGGCTAACAATATCGCACTCAATGCAGGCTTTAGAAGTTGTTCATAAGTAAGAACAACTTCTTAAAATTGCAGAGTTTCTAGCAAGAACTTTAGTGAGGATGAAGTTGTGAATTGGATCGTGATTGTTGCTCTACRAAGTGTGTGTGTGTAGCTTACTTAAATTTTTGTGTGTTAATTGGTGGCTGCTGAGTAGTGTATTGATGCTAAGTAGGTACTTTTAAGTAGGTGGTAGTAGGTAAGTTTA
TARGET=517,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01770 Ps_contig_mira-and-tgicl-ass_franssen_16374
SEQUENCE=TTCGATGAAGTTTCCATGGAACGCAGCAAGAGCTTCATCAACTCCTTGCAGGTTCTCTCTCGGATGATTCTCACTTTTTCTCTCTCTCTTTTTTTTTCTGTGAGTTTTTAATTTTATTTTTATATTTATATATATTTTTTTAAGTTCAGATCTGTATTTGTTTCAATTTTTTTGGTGGAGTTTGTATCTGACACATCTAGTTGTGTATGTTGACAATTTAGTTTTGAAATTGTGATTTTATTCAAAGACTT
TARGET=79,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4848
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=1.459198
PRIMER_LEFT_SEQUENCE=AAGAGCTTCATCAACTCCTTGC
PRIMER_RIGHT_SEQUENCE=TGTGTCAGATACAAACTCCACCA
PRIMER_LEFT=27,22
PRIMER_RIGHT=194,23
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=60.459
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=1.5119
PRIMER_LEFT_1_PENALTY=0.052729
PRIMER_RIGHT_1_PENALTY=1.459198
PRIMER_LEFT_1_SEQUENCE=CAACTCCTTGCAGGTTCTCTCT
PRIMER_RIGHT_1_SEQUENCE=TGTGTCAGATACAAACTCCACCA
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=194,23
PRIMER_LEFT_1_TM=60.053
PRIMER_RIGHT_1_TM=60.459
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=1.6710
PRIMER_LEFT_2_PENALTY=0.211806
PRIMER_RIGHT_2_PENALTY=1.459198
PRIMER_LEFT_2_SEQUENCE=CAGGTTCTCTCTCGGATGATTC
PRIMER_RIGHT_2_SEQUENCE=TGTGTCAGATACAAACTCCACCA
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=194,23
PRIMER_LEFT_2_TM=60.212
PRIMER_RIGHT_2_TM=60.459
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=1.8934
PRIMER_LEFT_3_PENALTY=0.025566
PRIMER_RIGHT_3_PENALTY=1.867802
PRIMER_LEFT_3_SEQUENCE=AAGAGCTTCATCAACTCCTTGC
PRIMER_RIGHT_3_SEQUENCE=TGTCAGATACAAACTCCACCAA
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=192,22
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=58.132
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=2.0074
PRIMER_LEFT_4_PENALTY=0.025566
PRIMER_RIGHT_4_PENALTY=1.981846
PRIMER_LEFT_4_SEQUENCE=AAGAGCTTCATCAACTCCTTGC
PRIMER_RIGHT_4_SEQUENCE=GTGTCAGATACAAACTCCACCAA
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=193,23
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=59.018
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_01771 Ps_contig_mira-and-tgicl-ass_franssen_16387
SEQUENCE=GATGTTGACGTCGTTTAACAGTTTCATTGTCGTTTCAATTTTCACCGAGATCTATCCTGCGTCATCCTCATTCATTCATCTCAAGAACCCTAACCTGCAAGCAATGTTACATASGCATGAAATCTTCCTAAACCAATGGTGGCACCCAAACGACACTTCCTCCCACTCATCACCCTCGCCGCATTCATCATCTTCTACACTTACCACAACACTTCTCT
TARGET=68,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01772 Ps_contig_mira-and-tgicl-ass_franssen_16391
SEQUENCE=GGGTTAAAGGGGGTTTTTAGGGGTTAAAAGGGTAAAAAGGGGAAAAGTTTAAAGGGGTTTAGGGTAAGTTAGGGAGGGAAAGGAAAGGAAAGGAAAGTACTGGATCGTGATTATGAGTATGGTTGAAGGTTGTTANAGTGAGAAGAATCTGGAAAGTTGTTAGAATTTTTGGTAGGTTTCTGTTGAAGGATTKAGGGCRAAAGCGATTTTGGAATGAGAGGGAAAATTTTWCTGAAACCAATGATTGGCAATGATTGCAGCTTATATAGCAGGATCTAAGGGGCAAATTGGAGAATTAAATCTGAAATGATTCAATCTGCACCATCCAGCTGGCATTAAGTGAAGGACATGGTTGATTACCAAGTGCAATCACAGAGTTTCTCCAAGTGGTCAAAGCTGAATCTGCAGCAAGGCTTCTCCTGTAGACTCCTTCTTGTTTTCCAATGTGTTGATTTGTGGGTCGCTTCCTGGATTCACTATGGAAACTTGAAGCTATTTTCGGGCCCAAAGGAAACATTGAGTGACGATGCCACCAGGTTTGTTTAGAAGTTAGTGACACATAGCCTTGTGATGAATG
TARGET=77,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01773 Ps_contig_mira-and-tgicl-ass_franssen_16406
SEQUENCE=TAATAGTGAAGATAGGCCTGGGTTAGGTTTAGGTCTTGGTTTTGGGTCTGCTAGTACGTCTGGGTCTGGTTTAGGTTTTAATTATGGTCGTGGTGCTGCAAATGGTTTTGATAGGAATGATGATGATAATGATAATGATAATGATAATGATAATGGTGATGATAAATTTTTGCCTACTGAGTTTGGGAAGAAGATTAAGGAGGGAGCAATGAGGAGGGAGAAGGAGAGATTGGAGAAGAAGAAGAAGGTRCAGAAGCAGGGGCTGGGTCTAGTTCATGAAGGATCTGTGGATGTTGGGAAATTTGAGAGTCATACGAAGGGGATCGGAATGAAGCTGCTGGAGAAGATGGGTTATAAAGGGGGTGGTCTTGGGAAGAATGAGCAGGGTATTTTAAATCCTATTGAGGCAAAATTGAGGGCTAAGAATTCTGGTCTTGGATTTAATGAATCGAAGGAGACTAAAACACCGTTGCCGGCTTTGCAGACGGAGAAGACATTGCCGGGAGGAGCCATGCAGCCTACAGTTGGAAGAACGAAAGAACGCTCCTGGTT
TARGET=234,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01774 Ps_contig_mira-and-tgicl-ass_franssen_16407
SEQUENCE=CAAAGAAAATCCCGCCGCTGCACCGTCCGCCGTCGCCCACCCCCTTTTCGTTATTTATCAGTTTATTCTTCGAGTCGTTTTAAGATGGATGAAGATCAAGAGATGGAGAAATTCGGGATGGAGAATGATTTTGAAGGTGGTAGATGGGAAGGTGGYGAGTTCTACTACCGAAAGCGTAAAGAGAAACGGACTCAAACCAAAGACGATGTACTCTATGGAGTTTTTGCTGATTCTGAGGATGACGACGATGATGAGTATTCCAGTAGAAAGAGAAGGAAAGACCGTGATTTCTCGAAGAAACAGGACCTCACTAAACCGGTGAATTTCGTTTCTACTGGAACTTTGCCTAATCAGGATAAGGTTGATGAAATTCCAAAAGAAAAAGAATGAGCAGGATAGTTATAATAGTGAAGATAGGCCTGGGTTAGGGTTAGGTTTAGGTCTTGGTTTTGGGTCTGCTAGTACGTCTGGGTCTGGTTTAGGTTTTAATTATGGTCGTGGTGCTGCGAATGGTTTTGATAGGAACGATGATGATGATGATAATGATAATGGTGATGATAAATTTTTGCCTACTGAGTTTGGGAAGAAGATTAAGGAGGGAGCAATGAGGAGGGAGAAGGAGAGATTGGAGAAGAAGAAGAAGGTGCAGAAGCAGGGGCTGGGTCTAGTTCATGAAGGATCTGTGGATGTTGGGAAATTTGGAGAGTCATAACGGGAAAGGGGAGTACGGAATAGGAA
TARGET=527,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01775 Ps_contig_mira-and-tgicl-ass_franssen_16407
SEQUENCE=CAAAGAAAATCCCGCCGCTGCACCGTCCGCCGTCGCCCACCCCCTTTTCGTTATTTATCAGTTTATTCTTCGAGTCGTTTTAAGATGGATGAAGATCAAGAGATGGAGAAATTCGGGATGGAGAATGATTTTGAAGGTGGTAGATGGGAAGGTGGYGAGTTCTACTACCGAAAGCGTAAAGAGAAACGGACTCAAACCAAAGACGATGTACTCTATGGAGTTTTTGCTGATTCTGAGGATGACGACGATGATGAGTATTCCAGTAGAAAGAGAAGGAAAGACCGTGATTTCTCGAAGAAACAGGACCTCACTAAACCGGTGAATTTCGTTTCTACTGGAACTTTGCCTAATCAGGATAAGGTTGATGAAATTCCAAAAGAAAAAGAATGAGCAGGATAGTTATAATAGTGAAGATAGGCCTGGGTTAGGGTTAGGTTTAGGTCTTGGTTTTGGGTCTGCTAGTACGTCTGGGTCTGGTTTAGGTTTTAATTATGGTCGTGGTGCTGCGAATGGTTTTGATAGGAACGATGATGATGATGATAATGATAATGGTGATGATAAATTTTTGCCTACTGAGTTTGGGAAGAAGATTAAGGAGGGAGCAATGAGGAGGGAGAAGGAGAGATTGGAGAAGAAGAAGAAGGTGCAGAAGCAGGGGCTGGGTCTAGTTCATGAAGGATCTGTGGATGTTGGGAAATTTGGAGAGTCATAACGGGAAAGGGGAGTACGGAATAGGAA
TARGET=630,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01776 Ps_contig_mira-and-tgicl-ass_franssen_16410
SEQUENCE=TCCTATAAAACCGAATATTATAAATTTATGACCTATTCTTGCTGCGAATAAATAAWCAATTTGATCTATAAAATATCATTCATTTTCAATTTGATCCTTACGAGTTTCCATAGTTGATAGATCAAAATGAATAGAAAGTGATAATTTATAAACTAAATTGATGTTTTATTCGTCCTGTTTACTATACAATATAAGTGAAAGTGTTAATAATGTAACGTTTGATTAGTTAAACATCAAAGGGTGTTGGGTCTCTGTTGCTTTTCTTGCTTGCGCCTCCATGGGACCACTTGCTTAAAGCTTCCATAAATGCTTGCCAAAAAATGTGAACTTCCATGTGAGAAGCTTGAGAAGCTTCCTCTTCTCCTAACCCTTGTAATCTTCACTTTATCACTACCATCCACACTCCAACTTGAAAAGTCGAAACTACCCTCACGATCAATTCTATTATTTTTCTTCAACYCVACCCCACCTAACTAAGAACCCAAAAACTACAACAACTACTACCTTATTATTATTATCCCTATACGAGGTCGTAAACG
TARGET=506,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01777 Ps_contig_mira-and-tgicl-ass_franssen_16411
SEQUENCE=ACCCACGGGAAGGCCTAAGCCTAGGCCGAGTGACAGAGGGTCGGAACCGAAAGAAAGAGAGACCAGCGTTGTTAGAGCTTTGGAACTTCCTCCGAGGTTGTTGTTGTCTTTGGAGAGTAGTAACATTAACGCGCCGTCACCGACAACTGTTTTGGAGGGACCTTACGTTGGACGCGCCGCGTCGTTTACGACCTCGTATAGGGATAATAATAATAAGGAGAGTGTGAGTTTTGGGTCTAGTAGGTGGGGTGGGTTGAAGAAAAATAATAGAATTGATCGTGAGGGTAGTTTCGACTTTTCAAGTTGGAGTGTGGATGGTAGTGATAAAGTGAAGATTACAAGGGTTAGGAGAAGAGGAAGCTTCTCAAGCTTCTCACATGGAAGTTCACTATTTTTTTGGCAAGCATTTTATGGAAGCTTTAAGCAAGTGGTCCCATGGAGGCGCAAGCAAGAAAGCAACAG
TARGET=204,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0541
PRIMER_LEFT_PENALTY=0.045851
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=GCGTTGTTAGAGCTTTGGAACT
PRIMER_RIGHT_SEQUENCE=CATCCACACTCCAACTTGAAAA
PRIMER_LEFT=65,22
PRIMER_RIGHT=316,22
PRIMER_LEFT_TM=59.954
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.0541
PRIMER_LEFT_1_PENALTY=0.045851
PRIMER_RIGHT_1_PENALTY=0.008257
PRIMER_LEFT_1_SEQUENCE=AGCGTTGTTAGAGCTTTGGAAC
PRIMER_RIGHT_1_SEQUENCE=CATCCACACTCCAACTTGAAAA
PRIMER_LEFT_1=64,22
PRIMER_RIGHT_1=316,22
PRIMER_LEFT_1_TM=59.954
PRIMER_RIGHT_1_TM=60.008
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.0899
PRIMER_LEFT_2_PENALTY=0.081670
PRIMER_RIGHT_2_PENALTY=0.008257
PRIMER_LEFT_2_SEQUENCE=GAGACCAGCGTTGTTAGAGCTT
PRIMER_RIGHT_2_SEQUENCE=CATCCACACTCCAACTTGAAAA
PRIMER_LEFT_2=58,22
PRIMER_RIGHT_2=316,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=60.008
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.1443
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=GAACCGAAAGAAAGAGAGACCA
PRIMER_RIGHT_3_SEQUENCE=CATCCACACTCCAACTTGAAAA
PRIMER_LEFT_3=43,22
PRIMER_RIGHT_3=316,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.2104
PRIMER_LEFT_4_PENALTY=0.045851
PRIMER_RIGHT_4_PENALTY=0.164560
PRIMER_LEFT_4_SEQUENCE=GCGTTGTTAGAGCTTTGGAACT
PRIMER_RIGHT_4_SEQUENCE=AAAGTCGAAACTACCCTCACGA
PRIMER_LEFT_4=65,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=59.954
PRIMER_RIGHT_4_TM=60.165
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_01778 Ps_contig_mira-and-tgicl-ass_franssen_16413
SEQUENCE=ACTTCTAGAAAACAAACATTTCCTCCCACCATTATCCTTCAGAGATGAATCAGTTAAAGGGCTATTCTTACCTTTATCATCATTTTTCTAGTCCATTTGAGTTAGCATTCTCTTTAGAGTTTGAAGAAGACATAAAAGAATAGAGAAAGGCAGTGAAGGACGAAGTAATCAGGTCCATCAACTAAATCATGATTGTTATTATTACCCTCTTCAATCACTTATTTTAAGCTCACCTATTTCTTCAGAAGAAGAAGATGGAGGATGGTTGG
TARGET=244,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01779 Ps_contig_mira-and-tgicl-ass_franssen_16443
SEQUENCE=CAAAAAAGAAAAATATTTTCTCTCCTCTTCAACCCTAAATCGCCGCCACCCTCTTCTTCCTCACTCACAAAAGCTAGCGGCTCACTCTCTAAAAAACTTCTTCACTGACAAAACCTAGCCGCCTCCCCCCTTCTTCCTCACAAAACCTAGCCGCCACCTTCATTTTCATCATCTCCTTCACCGAAGCCCTTCTTCCACACACTCACTCTCCGCCGCCACCCTTCATTTTCTCCAACCTTCACTCCACGAAACCTACACCAAATCATCAGCCTTCACCTTCATCAACCCCAAACCCGGAATCCCAACCGGTTCACGCAAAAGAACTCACTCCTCCTCACAATCCTCTTCCGCCTCACCAACACAACTGTCAAACCTTCGTCATCAACCAAATGACGCTCATCCCAACCGACCGTTTCTAGAAGCTCATTAACTCTGGCCACCAAATCGGTACAGAAACGGGAGCGTTATGCCCTGGATCCGTTCGCGTTCGCATCGGCATCATCACGGTTGGCGACGGGAAGAAAATGAAGAACAATGCTATGGTTTGTAACAGTGGTCACTTTTCCTTCTCCGGTTTCGTTTTCTCTCTCCTCGCCGATCCCAGATCCATCAAGAAAAGTTGGAAAAGATGAAATAGGACCAAACGGGATGCGAAGCAGCCTCTGGAGATCCTATGCAGTGCGTCAACAACTGGGATTTTTGTTTTTTCCGGAAGCGCATCTACCATGAACCGTTGCTCTAAGTGCCACAAAGACATGATGTTGAAACAGGAGCAGTGGAATTGAACCTGCTATTGACTGCCAATGTGGCAATCTCAGTCGATCCAGTGGAGCCCATAACTATCTCGGCACAACCGTTAGTTGCTTCTAGCTTAAAGGGGAGATTGGAGAAGAAGCTCAAGGATGGTCCTAAATGGTGCTCCAGCTGCAATAAGCGGTTTGGTTTTGACACGATTTATTTGTCTATGTGATTAACTTGTGTTATTTATTTTCTGTGCTTTACATCGCTACTCAGACAAACATGGCTGTTCATTTGACTACCACACTGCTGGACGGGATGCAATAGCCAAAGCAAACCTGGTTGCCATAGCTACTTCGCTATCGGTATCGAGCAGTCGAAACCCCAACAACAACAACAACAGTTTCATCTTCGCCAAGCAAGAAATAAACAGACAAACTCAACGCCCGACCAACCCCTTATAAATTAAAAAA
TARGET=1124,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8882
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.850848
PRIMER_LEFT_SEQUENCE=GGCTGTTCATTTGACTACCACA
PRIMER_RIGHT_SEQUENCE=TAATTTATAAGGGGTTGGTCGG
PRIMER_LEFT=1022,22
PRIMER_RIGHT=1205,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.149
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.8882
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.850848
PRIMER_LEFT_1_SEQUENCE=TGGCTGTTCATTTGACTACCAC
PRIMER_RIGHT_1_SEQUENCE=TAATTTATAAGGGGTTGGTCGG
PRIMER_LEFT_1=1021,22
PRIMER_RIGHT_1=1205,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.149
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.8882
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.850848
PRIMER_LEFT_2_SEQUENCE=CAGACAAACATGGCTGTTCATT
PRIMER_RIGHT_2_SEQUENCE=TAATTTATAAGGGGTTGGTCGG
PRIMER_LEFT_2=1011,22
PRIMER_RIGHT_2=1205,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=59.149
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=1.0661
PRIMER_LEFT_3_PENALTY=0.215254
PRIMER_RIGHT_3_PENALTY=0.850848
PRIMER_LEFT_3_SEQUENCE=TTTGACTACCACACTGCTGGAC
PRIMER_RIGHT_3_SEQUENCE=TAATTTATAAGGGGTTGGTCGG
PRIMER_LEFT_3=1031,22
PRIMER_RIGHT_3=1205,22
PRIMER_LEFT_3_TM=60.215
PRIMER_RIGHT_3_TM=59.149
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=1.1271
PRIMER_LEFT_4_PENALTY=0.276218
PRIMER_RIGHT_4_PENALTY=0.850848
PRIMER_LEFT_4_SEQUENCE=ATAAGCGGTTTGGTTTTGACAC
PRIMER_RIGHT_4_SEQUENCE=TAATTTATAAGGGGTTGGTCGG
PRIMER_LEFT_4=929,22
PRIMER_RIGHT_4=1205,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=59.149
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=277
=
PRIMER_SEQUENCE_ID=SSR_01780 Ps_contig_mira-and-tgicl-ass_franssen_16444
SEQUENCE=TCCTAAATAGGTGNTCCAGCTGCAATAAGCGGTTTGGTTTTTGTACACGATTATTTGTCTATGTGATTAACTTGCKTATTTATTTTCTTGTGCTTTACATCGCTACTCAGACAAACATGGCTGTTCATTTGACTACCACACTGCTGGACGGGATGCAATAGCCAAAGCAAACCTGGTTGCCATAGCTACTTCGCTATCGGTATCGAGCAGTGAAACCCCAACAACAACAACAACAGTTTCATCTTCGCCAAGCAGAATAAACAGCAAACTCAAGCCGCACCCTCTATAAAATAATAATCGGTAGTATTGCAGTTTTGTTTGTACACTGTTGTAGTATTTTGGTTTTTGTTTTTTATTTGTGTTTCGGTTGGATATACTGCTGTGTTGTGAATATTGGAAGTGTTGTTGTTAGACTTTTTTTGGCATATTCGCTTACAGCTGTATGAACTTAGGAGTTGTGTTTGATTCTTTTATCTCACAGTTATGATTTTTGTTCATATCATATTACACTTCTGAGTTCACGTATTAAAGK
TARGET=219,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01781 Ps_contig_mira-and-tgicl-ass_franssen_16449
SEQUENCE=AWRACAAAAAATCAACATCATTCATATCGGTATCAAACAATTCATTTATATCATAAACTACACAAATAAACTATCAAGGTTTCAAATTTAATCAATTGTCAAAATCCCAACGATAACAAATCCATTTCTCAAACTCAACTACTAGAAACAACCTCATCCTCCAACCCTTTCCTCTTAACATACTCATTCAAAATAGCAATCCTAGCCGTTTCAAAAGCAAAATAACCCAAAGCAGAAAAACAAGCACTATGAAGAACCCTAGGACCCATTCCACGCGTAAAACCAACCCACCCTTCTTCCTTCAAAATCTCCCTAATCGTAGACGAAACCCCTCCATACATAACCGCCGCAACCTTCCCAACCGCCTCATTACGCGCCTGCGTCATCAACCTCGTCTTCACCACATCCAACGGCGTCGTAATCGACGCCGATATCGCACCCGCCAACGCCCCACAAATCACACTCTGAATCGGTTCCAAATGATTTTTTTTCGTCTCTCTCATAACCGCTAGTTTCAAATACTCAAAAGAAGAATAACTTAACACACCAGCAGGTAAATTTCTCAACAACGTAGCTGAATAACCGGCGTATAAACCCATTACACCGTCGTTTTGAAGAATTTTAATCAAAACTTCATAAGATCTACCTTTCGCACCTGCTTGCATTCTCTGTGTTATCAACTCTTTCGGTACCATTATCGCAGACGACAAAACATTTCCTAAAGCACCAGCAGCAGGAGGAATCAAAATTTTCGGAAAATTTTCTTGTTTAGAGAGAAACGACTTACCGAACTCGCAAGTTCCGAAGTACACGGCTGAGGAAGCGGTGGATCCGACGACAACAGCGGAGAAGCCGCGGTAAAACCCTAGAATTCCGTTTGTGGAGAATGTTTGGGAAACTGCGTCGAGGGTATTTTTGTAAATCTGCGAAGCGCCTTTGGTTTGCATCTTGGTTTTGATTGTGTCGAGAGGGTGGAGACAAGCGTAAGTGAATGCGCCGGCGATTCCGCCGCCGGCTGCGCCGATGAATGCACGTTCGAAGACAGTTAGGTTTTTTAAGAGGGTTTGGGACTTTGGACCGGGTTTGAACCGGGGTTGGGTTGGGTTGGAGATTGAGGCGAAGGGGTTGGTGGTGGTGTGGGTGAGAGTGAGGGTTGTGAAGTGGTTGATGAGGCCATTGAAGTCGTTGAGTGAGGTTGAGAGTTCGGTGGGATTTGGAGATGGGAGACCGAGAGAAGATGAAATCCTGGCTTCCATGGAAGACATGAGATGAGTTAGTGCGGAAGTGGTGACACGAATGCGCGAGAAATTAAACTACCATCATCTTTTCC
TARGET=1092,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01782 Ps_contig_mira-and-tgicl-ass_franssen_16459
SEQUENCE=CTGCATTATGCACTCAAGGAACATTACCTTGTTCGCGAAGGCGATCCAGTGAACGAAATGCTCTTCATAATTAGAGGCCATCTTGATTCTTACACCACAAACGGAGGCCGTGACGGATTCTTCAACTCATGCCGTATCGGTCCAGGTGATTTCTGCGGCGAAGAACTATTGACATGGGCCTTAGATCCAAGACCAAGTGTGATACTCCCTTCATCGACAAGAACAGTTAACGCTGTCTCAGAAGTAGAAGCGTTTGCGCTCATAGCTGAAGATCTTAAGTTTGTTGCATCGCAATTTAGAAGATTACATAGTAAACAGCTTAGGCATAAGTTCAGATTTTATTCACACCAATGGAGAACTTGGGCTGCTTGTTTTATTCAAGCTGCATGGAGAAGGCACAAGAAAAGAAAAGAAGCTGCTGAATTGAGGGCTAAGGAGAATCTTATGGTAGCTTCAGAGACTGAGAGAGACAAAAAAAATATGGAACAGGTTTTGTTGTTTATGAAACAAGGGTGGCAAGGAGTACTAGGAAAGGTGTGCATTTGCAGAAGCCAACAGAACCTGTATTTTTCTGATGAATAATAATGAATGTACATTGTTTATTTTATAGTCTCTTTAATTGTCTAATTTGTTTGTTTTGGTATCATATTTGTKTGATATGAATTAGAGATAGGAATTGTTGTAACAAGTTGAGCAACTATGTTTAGTTACTAGAGTGCATGCACTTTGTGCTAAGATGGACTGAAGTTTCTCCATCCACTCTAAAAATGCAGAGTTYATCGAAAAGCTCTTTCGATAGTATATTTATATACAAAAATCACTTCTTCGAAGGTAATTTTMRAAAAACG
TARGET=400,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01783 Ps_contig_mira-and-tgicl-ass_franssen_16463
SEQUENCE=AGGTTAAACTAAAAAGGACGTTCTAGGGTCGATGGAAGGCCATATAGTGGAAGATTCCTTCTTGTTTTGCTCGTTCGACAGTTTCATCAACCTTTGACGTTGTTCGGTTATAAACAGAAATGGGGAATCCTTTGTCAGCAATGTTAAGGGCGAGATTTTGGCCCATAACAGCAAGTCCGGCAAGACCTATCCTCGTAAGTGTTGGAGCCATGATTTATTTCCGATTGAAAAAATGAATATTAAAATCAAAGTTTGAAACTTGAAATTCAATGGTCGAAGATGGCTAATGAAACCAACCAACCACCAACCTTTTTCGGAATGAAAAGGAACCCATCYTCGTATCC
TARGET=291,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01784 Ps_contig_mira-and-tgicl-ass_franssen_16467
SEQUENCE=TAGCAATTTAACCTTACCCTAACCGTAACCCAACGACTCATAACCTTACATAAATTTTACTAAGACATTAAGATGCCCCACGTTTCAGATGCCACTCTTCTTCTTTTTCTTCTCTTCAAACTAACACTAACAACAACCCATCCAGATTACATCTCTCCAATCTCGATAACTTTCTCCAAACTCAGAAGCCATTACAATCAACCACCACCACCACCACCCACCACCACCAACCCATCCAAAAAAAGTGACTCAAAGGAAC
TARGET=201,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.4949
PRIMER_LEFT_PENALTY=0.211336
PRIMER_RIGHT_PENALTY=4.283554
PRIMER_LEFT_SEQUENCE=CCCATCCAGATTACATCTCTCC
PRIMER_RIGHT_SEQUENCE=TTTTTGGATGGGTTGGTGGT
PRIMER_LEFT=136,22
PRIMER_RIGHT=241,20
PRIMER_LEFT_TM=59.789
PRIMER_RIGHT_TM=62.284
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=4.5934
PRIMER_LEFT_1_PENALTY=0.309858
PRIMER_RIGHT_1_PENALTY=4.283554
PRIMER_LEFT_1_SEQUENCE=ACGTTTCAGATGCCACTCTTCT
PRIMER_RIGHT_1_SEQUENCE=TTTTTGGATGGGTTGGTGGT
PRIMER_LEFT_1=79,22
PRIMER_RIGHT_1=241,20
PRIMER_LEFT_1_TM=60.310
PRIMER_RIGHT_1_TM=62.284
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=4.9256
PRIMER_LEFT_2_PENALTY=0.642030
PRIMER_RIGHT_2_PENALTY=4.283554
PRIMER_LEFT_2_SEQUENCE=CGTAACCCAACGACTCATAACC
PRIMER_RIGHT_2_SEQUENCE=TTTTTGGATGGGTTGGTGGT
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=241,20
PRIMER_LEFT_2_TM=60.642
PRIMER_RIGHT_2_TM=62.284
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=4.9256
PRIMER_LEFT_3_PENALTY=0.642030
PRIMER_RIGHT_3_PENALTY=4.283554
PRIMER_LEFT_3_SEQUENCE=CCGTAACCCAACGACTCATAAC
PRIMER_RIGHT_3_SEQUENCE=TTTTTGGATGGGTTGGTGGT
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=241,20
PRIMER_LEFT_3_TM=60.642
PRIMER_RIGHT_3_TM=62.284
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=4.9256
PRIMER_LEFT_4_PENALTY=0.642030
PRIMER_RIGHT_4_PENALTY=4.283554
PRIMER_LEFT_4_SEQUENCE=ACCGTAACCCAACGACTCATAA
PRIMER_RIGHT_4_SEQUENCE=TTTTTGGATGGGTTGGTGGT
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=241,20
PRIMER_LEFT_4_TM=60.642
PRIMER_RIGHT_4_TM=62.284
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_01785 Ps_contig_mira-and-tgicl-ass_franssen_16468
SEQUENCE=GGGAATATTCGGCTGTTTTGGAGAGAAGGCGCTGGAGGCGTTTTAGGTGACGTGGGGAGATTTCTTCCGCTGCTTTGATTTCCGGCCATAGTTTTGCTAGGTTGAGACCTTGAAGGTAAGTTTTTCCTTTTTTGTCCTTTGAGTCGCTTTTTTGGATGGGTTGGTGGTGGTGGGTGGTGGTGGTGGTGGTTGATTGTAATGGCTTCTGAGTTTGGAGAAAGTTATCGAGATTGGGAAGAGGAAGTTGAAGTGGTGGTTCTTG
TARGET=162,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0990
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=ATATTCGGCTGTTTTGGAGAGA
PRIMER_RIGHT_SEQUENCE=CAGAAGCCATTACAATCAACCA
PRIMER_LEFT=4,22
PRIMER_RIGHT=207,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=0.1107
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.015253
PRIMER_LEFT_1_SEQUENCE=ATATTCGGCTGTTTTGGAGAGA
PRIMER_RIGHT_1_SEQUENCE=CATTACAATCAACCACCACCAC
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=200,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=60.015
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=0.1118
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.016312
PRIMER_LEFT_2_SEQUENCE=ATATTCGGCTGTTTTGGAGAGA
PRIMER_RIGHT_2_SEQUENCE=GAACCACCACTTCAACTTCCTC
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=258,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.016
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=0.1294
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.033905
PRIMER_LEFT_3_SEQUENCE=ATATTCGGCTGTTTTGGAGAGA
PRIMER_RIGHT_3_SEQUENCE=TTCCTCTTCCCAATCTCGATAA
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=242,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=60.034
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=0.2412
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=0.145769
PRIMER_LEFT_4_SEQUENCE=ATATTCGGCTGTTTTGGAGAGA
PRIMER_RIGHT_4_SEQUENCE=CCACCACTTCAACTTCCTCTTC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=255,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=60.146
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_01786 Ps_contig_mira-and-tgicl-ass_franssen_16468
SEQUENCE=GGGAATATTCGGCTGTTTTGGAGAGAAGGCGCTGGAGGCGTTTTAGGTGACGTGGGGAGATTTCTTCCGCTGCTTTGATTTCCGGCCATAGTTTTGCTAGGTTGAGACCTTGAAGGTAAGTTTTTCCTTTTTTGTCCTTTGAGTCGCTTTTTTGGATGGGTTGGTGGTGGTGGGTGGTGGTGGTGGTGGTTGATTGTAATGGCTTCTGAGTTTGGAGAAAGTTATCGAGATTGGGAAGAGGAAGTTGAAGTGGTGGTTCTTG
TARGET=174,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1118
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.016312
PRIMER_LEFT_SEQUENCE=ATATTCGGCTGTTTTGGAGAGA
PRIMER_RIGHT_SEQUENCE=GAACCACCACTTCAACTTCCTC
PRIMER_LEFT=4,22
PRIMER_RIGHT=258,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=60.016
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=255
PRIMER_PAIR_PENALTY_1=0.1294
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.033905
PRIMER_LEFT_1_SEQUENCE=ATATTCGGCTGTTTTGGAGAGA
PRIMER_RIGHT_1_SEQUENCE=TTCCTCTTCCCAATCTCGATAA
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=242,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=60.034
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.2412
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.145769
PRIMER_LEFT_2_SEQUENCE=ATATTCGGCTGTTTTGGAGAGA
PRIMER_RIGHT_2_SEQUENCE=CCACCACTTCAACTTCCTCTTC
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.146
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=0.3489
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.253482
PRIMER_LEFT_3_SEQUENCE=ATATTCGGCTGTTTTGGAGAGA
PRIMER_RIGHT_3_SEQUENCE=TCCAAACTCAGAAGCCATTACA
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=215,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=59.747
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.3862
PRIMER_LEFT_4_PENALTY=0.369874
PRIMER_RIGHT_4_PENALTY=0.016312
PRIMER_LEFT_4_SEQUENCE=AATATTCGGCTGTTTTGGAGAG
PRIMER_RIGHT_4_SEQUENCE=GAACCACCACTTCAACTTCCTC
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=258,22
PRIMER_LEFT_4_TM=59.630
PRIMER_RIGHT_4_TM=60.016
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_01787 Ps_contig_mira-and-tgicl-ass_franssen_16478
SEQUENCE=TGTGACAATATCAATAAGGCACATCTTGATTACTTTTATTGGTTACTTTCTGGATTAAGTGTGGTGGGGTTTACTTTCTTCATTTATTTTTCTAAATCTTATGTTTATAATAACAAAGGTATCATCAAGATAGTCAATCGTTAGTTTTATTTATTTATTTATTTATTATGTCATATTTGTATTGATGGCAGTTTGATATGCATGGTACAATAATGCATGATAATGGAAGGTAGGGACTTAACGGTAATATAATTGAATTAAAACTTTTTAAATAATATATCTATTTTAAG
TARGET=146,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6006
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.580833
PRIMER_LEFT_SEQUENCE=GGATTAAGTGTGGTGGGGTTTA
PRIMER_RIGHT_SEQUENCE=CCGTTAAGTCCCTACCTTCCAT
PRIMER_LEFT=51,22
PRIMER_RIGHT=243,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.581
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=193
PRIMER_PAIR_PENALTY_1=0.6501
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.630249
PRIMER_LEFT_1_SEQUENCE=GGATTAAGTGTGGTGGGGTTTA
PRIMER_RIGHT_1_SEQUENCE=GTACCATGCATATCAAACTGCC
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=207,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=59.370
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=0.8353
PRIMER_LEFT_2_PENALTY=0.254467
PRIMER_RIGHT_2_PENALTY=0.580833
PRIMER_LEFT_2_SEQUENCE=TGTGGTGGGGTTTACTTTCTTC
PRIMER_RIGHT_2_SEQUENCE=CCGTTAAGTCCCTACCTTCCAT
PRIMER_LEFT_2=59,22
PRIMER_RIGHT_2=243,22
PRIMER_LEFT_2_TM=60.254
PRIMER_RIGHT_2_TM=60.581
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.8353
PRIMER_LEFT_3_PENALTY=0.254467
PRIMER_RIGHT_3_PENALTY=0.580833
PRIMER_LEFT_3_SEQUENCE=GTGGTGGGGTTTACTTTCTTCA
PRIMER_RIGHT_3_SEQUENCE=CCGTTAAGTCCCTACCTTCCAT
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=60.254
PRIMER_RIGHT_3_TM=60.581
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.8397
PRIMER_LEFT_4_PENALTY=0.258908
PRIMER_RIGHT_4_PENALTY=0.580833
PRIMER_LEFT_4_SEQUENCE=TGGTGGGGTTTACTTTCTTCAT
PRIMER_RIGHT_4_SEQUENCE=CCGTTAAGTCCCTACCTTCCAT
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=243,22
PRIMER_LEFT_4_TM=59.741
PRIMER_RIGHT_4_TM=60.581
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_01788 Ps_contig_mira-and-tgicl-ass_franssen_16481
SEQUENCE=TAACATACGTATAATAGTTAACAATAGCATGGTGTGTTGGCCAAAACCCAACGGAGTTAACTTAGATGTGCAGCTACAAGCAAACTTGGATTATGCTTGTGGGCAGGGGAGTTGTTATTGCAGCTCAATCCAACCAGGAGGAGCTTGTTTCGAACCTAACACATTAGCGAATCATGCTGCATATGCCATGAATCTCTTTTATCACACTGGGCAAAATCCATTAACTTGTGATTTCTCAGAGACAGCCACACTAACATCAAATAACCCAAGTTATAATAGTTGTGTTTATGCCGGAGGGAATGCATAATTTAAAAAACAAGAAAGTAGATAAAGGATGGTGGTGTTTTTAGTTTTGTGGTTGTTTTAATTCTTATTTATTTATTGTTTAAAAATGTTTTGGTCTTAAAAGTGTTCTAAAGTAAAACAATGGTGTCTGTACAGAGGAGGATCCTCTTCTCCTCTATTTCTGTTGTAAACGTTTGTCTCGGTTAGAAGCAACCTGCTGCATTTTTTTTTATGATGTGTGTTTGCTATCAAGTGTTGAGTCATCATTGAAATCTTGATGTCCATACTAATGTTGGTTCGAATAATATGATGTTTTTCAAANTAG
TARGET=371,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4060
PRIMER_LEFT_PENALTY=0.115769
PRIMER_RIGHT_PENALTY=0.290243
PRIMER_LEFT_SEQUENCE=ACACTGGGCAAAATCCATTAAC
PRIMER_RIGHT_SEQUENCE=CCGAGACAAACGTTTACAACAG
PRIMER_LEFT=203,22
PRIMER_RIGHT=487,22
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=59.710
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.4107
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.294975
PRIMER_LEFT_1_SEQUENCE=ACACTGGGCAAAATCCATTAAC
PRIMER_RIGHT_1_SEQUENCE=AGGTTGCTTCTAACCGAGACAA
PRIMER_LEFT_1=203,22
PRIMER_RIGHT_1=500,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=60.295
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.5269
PRIMER_LEFT_2_PENALTY=0.236697
PRIMER_RIGHT_2_PENALTY=0.290243
PRIMER_LEFT_2_SEQUENCE=CACTGGGCAAAATCCATTAACT
PRIMER_RIGHT_2_SEQUENCE=CCGAGACAAACGTTTACAACAG
PRIMER_LEFT_2=204,22
PRIMER_RIGHT_2=487,22
PRIMER_LEFT_2_TM=60.237
PRIMER_RIGHT_2_TM=59.710
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=284
PRIMER_PAIR_PENALTY_3=0.5317
PRIMER_LEFT_3_PENALTY=0.236697
PRIMER_RIGHT_3_PENALTY=0.294975
PRIMER_LEFT_3_SEQUENCE=CACTGGGCAAAATCCATTAACT
PRIMER_RIGHT_3_SEQUENCE=AGGTTGCTTCTAACCGAGACAA
PRIMER_LEFT_3=204,22
PRIMER_RIGHT_3=500,22
PRIMER_LEFT_3_TM=60.237
PRIMER_RIGHT_3_TM=60.295
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.5362
PRIMER_LEFT_4_PENALTY=0.115769
PRIMER_RIGHT_4_PENALTY=0.420406
PRIMER_LEFT_4_SEQUENCE=ACACTGGGCAAAATCCATTAAC
PRIMER_RIGHT_4_SEQUENCE=ACCGAGACAAACGTTTACAACA
PRIMER_LEFT_4=203,22
PRIMER_RIGHT_4=488,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=59.580
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_01789 Ps_contig_mira-and-tgicl-ass_franssen_16493
SEQUENCE=GAAAGAAAGAWTWGATGATAATGTTGAAGAAAGAAATGATGATGAAATGGAGGATGTGGAGAATGCGGAGGAGGAGGAGGGTGTTGATGATCCAAGGTGGGATAACATAAGGAATAGGTTCCAAGGTGGATCGGAAAGACCTGAATATCGAAGATGGGATAGTAATGAAAATTGGGGTAGAAAGACATGGAGAGAGGCTACTGAATCCACTGTGCCCAAGGTTGTTGGTGAAGGAATCTATGGGGTTGGTCCAGTTTTGGCTGCATTGTCAGCTGGAAGAAGGGAGTTCTATGCATTGTATGTGCAACAAGGGTTGGATTTGAGTAGCAACAACAGGAAAAAGAAAGACAAGAAAGGGTTTGAGAGGGTTCTTAAGATTGCTGAAAAGCTCAATATAAGTATAAAAGAAGCATCAAAACATGATCTGAATATGGTAGCGGACAACCGACCTCATCAAGGTTTGGTATTAGACGCTTCGCCGCTTGAGATGG
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01790 Ps_contig_mira-and-tgicl-ass_franssen_16498
SEQUENCE=CTTCTTCACCATNANCAANTACCACCCNGGCCAATAATGGNAACCMCCATTGCCGGAAACTGAAAAGTTGAATCCCGGAGATCCTCCTCCGGCGCCGGAAGATTAAGATCCAATTCTAGAATATTTCGAGTCTTCCCATGAACATCACCAACGTTGTTGTTGTTGTTTTTGTTAGTACACGCTCTGTGTCTTCTCATATGACCACCCAATGCTTGACCTGATGTAAACTCCGCGCCGCAAATCGAACACTCATGAACCTTGTTGGATCTATTGGGTTTGTTATTGTTTCCATGGCCATGGAAGTTTAGATTACCACCACACCTTAATTCAAGAGAAACATGAGGAGGAACAAGAGGACTAATAGTAGAAACATTCTTGTTATGAATATGATTGTTTTGTTCTTCGGTTTCACCTGCGGAAATGATTTCTTTCATCTTGGGTTTCTTGTGACTTGCTCTGTGTCCACCCAACGCTTGAAATGAATGGAAAAAACGGTTACAAGTTTTGCACTCATAGCTTTCAAAACCGTTTCTTGTTGCCTTTTCTGTTACTATATTGTTATAACCACCGTCTGGTTGACGGTGGTTATGACAACGTTTCTCTTCTTGTCCTCCGGTTCTTCCTTGAGCTAGGRGAATCAAACAGTTAGCCATGTCAGCCTCCTCATCGTACGCAGTGCTATCGAAGGTGGTGGATGAGAACG
TARGET=153,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01791 Ps_contig_mira-and-tgicl-ass_franssen_16501
SEQUENCE=TACTTTTTAGTTGAATAGTTCTTTCTCCAGCATTGTCCTTTCAATCTCAAAAGGAAATCCAACAGGAATTTCAACTTGTTCAGTAGCTTGTTCATCCTCGAGGGCGCAATTGATTTCAGATTGAAGTACTTGAACAAGATTAGCATCTGGGGACGACTTATTTGCGACGGCGTTAGCGAAGCTACGAGAGGGTACAAAAGGAGTCATCTCTTGGTGAGGAAGGAGTTTGACAGAGAGTGCGCTGTGGAAGGTTCTGGAAGAAGAAGAAGCCACCGTGCGGCGAGCAGCCAGAGGAATTACGGCGGCGGTGGCACGACGTAGGACGGTGTACATGGCCATTTGAAAGTTGAGTAGAGACTGGTGCAGGGTTTAGTGTTAGGGTTTTCCSCGAC
TARGET=257,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01792 Ps_contig_mira-and-tgicl-ass_franssen_16506
SEQUENCE=GATCATCATCATCATCGTGCAAAGATATGGCGGATTCTATTTCATTCTTCGCCTCTCTTCCYGAAGATATCAACTTCAAAATTGCTTCACTTCTTCRGGTTYCAATTTTCTACTATTTTCCACTTCTTCATGCTTCATACAACTTTGA
TARGET=2,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01793 Ps_contig_mira-and-tgicl-ass_franssen_16509
SEQUENCE=TTATTCCTACCTTAACCGTTTTTCCTTTTCCTACTAATAAGACTAATTTTGTTTGTTTCTCCAAAAAACTCAGCACAGGAACACCCCCAACGCTAATACTTGACCATAATGCCACTGAACTATCCCCATGCTCCCCTACTATGCATGCCTGAACATCCTGAGCGTTGACCTGGAGGTGATCAGCAATGAGAAAACGAAAGCGAGAAGAATCCAAATTGGTACCCGACCCGATGATCCGGTTCGATGGAAACCCAGAAAGCTTCCATGCGATATAGGTGAGAACGTCAACGGGGTTAGACACGATCATGAGAATCGTTTCCGGCGAGTACCGGGCCAGAAGGGGTATGATTCCCTTGAAAAGAGCAAGGTTCCTCTGGAGAAGGTTAAGCCTAGACTCACCCGCGATCTGTCGTGCACCGGCGGTAACGATACACAGATCAGAGCCGGCGGTAACGGAGTATTCGACGGATGAGTTGATCTTGACGCGGGGGAGGAAAGCAGCAGCGTGCTGAAGGTCGAGCATCTCGCCGCGGAGCTTGTCGGGTTTGTTATCGACGATGACGAGCTCGTCGGTGAGGTCTTGAGTGAGGATGGTCTGAGCGATGGCCATTCCGACATTTCCGGCGCCGATGACTGAGATTTTGTTGTGGCGTTTAGTGATAGATGGAGGGGATGCGTTGGAGATGGGTTTGAAGAAGGACTGGGTTAAGTCAAGGCCTCCCGGGCCTAATGAACCAGAACTGCTTTTGTGCATAATGATTTGTGGTGTGAAGTGTGTGTGTGAGAGTATGGTTAAAGAAGG
TARGET=773,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01794 Ps_contig_mira-and-tgicl-ass_franssen_16512
SEQUENCE=TACTTCTTATTACCTTGCTATAAGATTCTTAAAGTAAGACAAAATTAGTTGTGGACAATCTATGAGATGAATAAAGCATTGTAATTGTTGTTGTTGTTGACTTGAGGCGTTGAGTTGTTGGAGTTGAGAGTGCACCACCCAACATTTGAGTATGAGTGATAAAAACAGTTCCACAAAACTACCATAAGAATGAGCAACAATTGTGAAACTGAAACTTTTGCTACATAAAATGCTCTCTCAATTGTTTTAGTTTTTGGAAGATGTTTTGGGTTGAAGATGATGATAGAAAGTGTTTTTTCTTGATTCGTTGATATTGATTATGTTAGAAGATAAATAAAATAAAATAAAATAATTAG
TARGET=85,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01795 Ps_contig_mira-and-tgicl-ass_franssen_16512
SEQUENCE=TACTTCTTATTACCTTGCTATAAGATTCTTAAAGTAAGACAAAATTAGTTGTGGACAATCTATGAGATGAATAAAGCATTGTAATTGTTGTTGTTGTTGACTTGAGGCGTTGAGTTGTTGGAGTTGAGAGTGCACCACCCAACATTTGAGTATGAGTGATAAAAACAGTTCCACAAAACTACCATAAGAATGAGCAACAATTGTGAAACTGAAACTTTTGCTACATAAAATGCTCTCTCAATTGTTTTAGTTTTTGGAAGATGTTTTGGGTTGAAGATGATGATAGAAAGTGTTTTTTCTTGATTCGTTGATATTGATTATGTTAGAAGATAAATAAAATAAAATAAAATAATTAG
TARGET=332,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01796 Ps_contig_mira-and-tgicl-ass_franssen_16514
SEQUENCE=GTCCGTTACTCTCGTCGGGGGAACCTCACCTTCAGGGACACAGACCACCCACCCACCCACCCCCATCCCGTTCCGTTCTCATCCCCAACTCAACCACCTTTCTTTCTTCCTTCAATCTCTTCCAAATTCAGTATCCAATTCTCTTTCAACCTTTCTCTCTTTTTCGACAAACGGAAAATCATTCCAATGACATCACCATCTACTACAATACCAATCACAGTAACCAACACGGACTCGTCACGTGACTCAAATACAAAGAAACCAAAGAACAAGAAGCATCATCAAGATTCTCAAGATTCTCAAGGTTCTCAGATTCAGATCAACCCTAAATGGAAATCCCAAGCGCAGCATCAAATCTACTCCGCCAAGCTTCGTCGAGCCATAACATCGCTCAACTCAACAACCACTCCTCGCCGTGGAAAAGACCGTCCGAGAAGCCGCCGACAGAGCTCTCGCCGTGACAGCCAAGGGAAGAACCCGATGGAGCCGAGCCATTCTGTATAGCCAGCTCAAACTCAAGTTCAGGAAGAATAAGAATAAGAAGAAGAAGATACATCGCGTAACCAAGAAGTCGAGAATCAATGTTTTCCGGTTGAAAGGAAAGGTGGTTCCCGGTGTACAGAGTAAAGCAAGGTTTCTTGGACGGTTGGTTCCTGGTTGTAAGAAGGAACCGTTATCGGTAATTTTGGAAGAAGCTATTGATTATATACCTGCGCTTGAGATGCAGGTTAGNGCCATGAGTGCTCTTTTCAATCTGTTATCGGCGTCTACTTCCGGCGCCGGTGACGGTTCTGGTTCCGGCTTAGTTAAAAACTCGTTTTTTGGGTTTTTCGGGTTTTAATTTGTATTTTAATTGTAATATACGTCAAGTTGTCCTTATTTTTTTTAATTTTTATTATTGTTATTATTTACTTA
TARGET=45,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4398
PRIMER_LEFT_PENALTY=1.259062
PRIMER_RIGHT_PENALTY=0.180765
PRIMER_LEFT_SEQUENCE=CTCACCTTCAGGGACACAGAC
PRIMER_RIGHT_SEQUENCE=CGTGTTGGTTACTGTGATTGGT
PRIMER_LEFT=24,21
PRIMER_RIGHT=230,22
PRIMER_LEFT_TM=59.741
PRIMER_RIGHT_TM=59.819
PRIMER_LEFT_GC_PERCENT=57.143
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=2.0003
PRIMER_LEFT_1_PENALTY=1.259062
PRIMER_RIGHT_1_PENALTY=0.741209
PRIMER_LEFT_1_SEQUENCE=CTCACCTTCAGGGACACAGAC
PRIMER_RIGHT_1_SEQUENCE=CCGTTTGTCGAAAAAGAGAGAA
PRIMER_LEFT_1=24,21
PRIMER_RIGHT_1=173,22
PRIMER_LEFT_1_TM=59.741
PRIMER_RIGHT_1_TM=60.741
PRIMER_LEFT_1_GC_PERCENT=57.143
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=150
PRIMER_PAIR_PENALTY_2=2.0960
PRIMER_LEFT_2_PENALTY=1.259062
PRIMER_RIGHT_2_PENALTY=0.836966
PRIMER_LEFT_2_SEQUENCE=CTCACCTTCAGGGACACAGAC
PRIMER_RIGHT_2_SEQUENCE=TCCGTGTTGGTTACTGTGATTG
PRIMER_LEFT_2=24,21
PRIMER_RIGHT_2=232,22
PRIMER_LEFT_2_TM=59.741
PRIMER_RIGHT_2_TM=60.837
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=2.1179
PRIMER_LEFT_3_PENALTY=1.937090
PRIMER_RIGHT_3_PENALTY=0.180765
PRIMER_LEFT_3_SEQUENCE=GAACCTCACCTTCAGGGACAC
PRIMER_RIGHT_3_SEQUENCE=CGTGTTGGTTACTGTGATTGGT
PRIMER_LEFT_3=20,21
PRIMER_RIGHT_3=230,22
PRIMER_LEFT_3_TM=60.937
PRIMER_RIGHT_3_TM=59.819
PRIMER_LEFT_3_GC_PERCENT=57.143
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=2.1615
PRIMER_LEFT_4_PENALTY=2.088170
PRIMER_RIGHT_4_PENALTY=0.073355
PRIMER_LEFT_4_SEQUENCE=GAACCTCACCTTCAGGGACA
PRIMER_RIGHT_4_SEQUENCE=GAGTCCGTGTTGGTTACTGTGA
PRIMER_LEFT_4=20,20
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=60.088
PRIMER_RIGHT_4_TM=60.073
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_01797 Ps_contig_mira-and-tgicl-ass_franssen_16514
SEQUENCE=GTCCGTTACTCTCGTCGGGGGAACCTCACCTTCAGGGACACAGACCACCCACCCACCCACCCCCATCCCGTTCCGTTCTCATCCCCAACTCAACCACCTTTCTTTCTTCCTTCAATCTCTTCCAAATTCAGTATCCAATTCTCTTTCAACCTTTCTCTCTTTTTCGACAAACGGAAAATCATTCCAATGACATCACCATCTACTACAATACCAATCACAGTAACCAACACGGACTCGTCACGTGACTCAAATACAAAGAAACCAAAGAACAAGAAGCATCATCAAGATTCTCAAGATTCTCAAGGTTCTCAGATTCAGATCAACCCTAAATGGAAATCCCAAGCGCAGCATCAAATCTACTCCGCCAAGCTTCGTCGAGCCATAACATCGCTCAACTCAACAACCACTCCTCGCCGTGGAAAAGACCGTCCGAGAAGCCGCCGACAGAGCTCTCGCCGTGACAGCCAAGGGAAGAACCCGATGGAGCCGAGCCATTCTGTATAGCCAGCTCAAACTCAAGTTCAGGAAGAATAAGAATAAGAAGAAGAAGATACATCGCGTAACCAAGAAGTCGAGAATCAATGTTTTCCGGTTGAAAGGAAAGGTGGTTCCCGGTGTACAGAGTAAAGCAAGGTTTCTTGGACGGTTGGTTCCTGGTTGTAAGAAGGAACCGTTATCGGTAATTTTGGAAGAAGCTATTGATTATATACCTGCGCTTGAGATGCAGGTTAGNGCCATGAGTGCTCTTTTCAATCTGTTATCGGCGTCTACTTCCGGCGCCGGTGACGGTTCTGGTTCCGGCTTAGTTAAAAACTCGTTTTTTGGGTTTTTCGGGTTTTAATTTGTATTTTAATTGTAATATACGTCAAGTTGTCCTTATTTTTTTTAATTTTTATTATTGTTATTATTTACTTA
TARGET=539,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3018
PRIMER_LEFT_PENALTY=0.095553
PRIMER_RIGHT_PENALTY=0.206212
PRIMER_LEFT_SEQUENCE=AGCCATTCTGTATAGCCAGCTC
PRIMER_RIGHT_SEQUENCE=AAAGAGCACTCATGGCNCTAAC
PRIMER_LEFT=489,22
PRIMER_RIGHT=748,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=60.206
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.50
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.75
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.3500
PRIMER_LEFT_1_PENALTY=0.095553
PRIMER_RIGHT_1_PENALTY=0.254467
PRIMER_LEFT_1_SEQUENCE=AGCCATTCTGTATAGCCAGCTC
PRIMER_RIGHT_1_SEQUENCE=TTCTTACAACCAGGAACCAACC
PRIMER_LEFT_1=489,22
PRIMER_RIGHT_1=665,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=60.254
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.3535
PRIMER_LEFT_2_PENALTY=0.095553
PRIMER_RIGHT_2_PENALTY=0.257927
PRIMER_LEFT_2_SEQUENCE=AGCCATTCTGTATAGCCAGCTC
PRIMER_RIGHT_2_SEQUENCE=TAGACGCCGATAACAGATTGAA
PRIMER_LEFT_2=489,22
PRIMER_RIGHT_2=769,22
PRIMER_LEFT_2_TM=59.904
PRIMER_RIGHT_2_TM=59.742
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=281
PRIMER_PAIR_PENALTY_3=0.3703
PRIMER_LEFT_3_PENALTY=0.095553
PRIMER_RIGHT_3_PENALTY=0.274768
PRIMER_LEFT_3_SEQUENCE=AGCCATTCTGTATAGCCAGCTC
PRIMER_RIGHT_3_SEQUENCE=TTTACTCTGTACACCGGGAACC
PRIMER_LEFT_3=489,22
PRIMER_RIGHT_3=627,22
PRIMER_LEFT_3_TM=59.904
PRIMER_RIGHT_3_TM=60.275
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=0.4514
PRIMER_LEFT_4_PENALTY=0.095553
PRIMER_RIGHT_4_PENALTY=0.355872
PRIMER_LEFT_4_SEQUENCE=AGCCATTCTGTATAGCCAGCTC
PRIMER_RIGHT_4_SEQUENCE=CCGATAACGGTTCCTTCTTACA
PRIMER_LEFT_4=489,22
PRIMER_RIGHT_4=679,22
PRIMER_LEFT_4_TM=59.904
PRIMER_RIGHT_4_TM=60.356
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_01798 Ps_contig_mira-and-tgicl-ass_franssen_16517
SEQUENCE=TCCATGAYYGKATCWTTTTTCACCTTTTCTCCTCACTCTAACTACTCAAAACTTCATTTTCCAGCTTATGACTTCAACTTCTAAAAGCTCATAACAACCTATAACTAACTCCTAACTTCCCTTCACTTCTAACTGATTTTTCTCCTCACCCCTAACTAACTAACTCATAACCTCTTCTAACTTCCCTACTTCGACATAAATAGCACCATTCAAAATCTCTTCATAAAAAACCTGCTAACAGACCCACCTCAATCGAATGATCCCACTGAATTAACTTTGAACCATTCAACCACAACCTCCACTATAGAAAACTCACTCCTCCAACCTACTACATAAAAACAAGTCTTCCTCACCCTTCATCCTCTTCTCTCAACCCTCTTCTCCAACGATCTTCCATTCACACAACCACCCTCATTGAACTGATGRCCCCTTCAACTGAGGCCAAAAAAACAACACCTTCCCTAACAACCTCCTTCAAGAATCATCTTTGACCTTATTCTGACCTTCCATTCTAACCYTTCAATCCCTCCRTCATCATGTAACAACTCCTAACCAGTTTCTTCTGAAACCCTTCTAACCAACTA
TARGET=152,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01799 Ps_contig_mira-and-tgicl-ass_franssen_16525
SEQUENCE=CAATCAAGCAGAAATCACAATTGGAGGAGGAGCTGAAGATACTTACCGAGAAATGTTCTGAGCATGAAGATCGAGCTACCACATACAGTCAACGCAGCCTAGAACTAGAAGGTTTAATTCAGAGCTCTCATTCCAAATCGGAAAGTTCTGAAAAGAGAGTGAGTGAGTTGGAGTTGCTACTTGAAACTGAGAAATACAGAATTCAGGAACTTGAACAACAAACAAGCACATTAGAAAAGAAATATAGTGAGTCAGAAGAAAATTCCAATAAACAACTTGAGAATGTGTCTTATCTGACATCAGAACTTGATGTGTTTCAAGCTCGAACCTCAACCCTCGAAAGTACACTGCAAGAAGCGAATGAAAGGGAAAAAGAGTTAAAAGAATCTTTAAATGCTGTGACGGATGAAAAGAAAAAGTTAGAAGATTCTTTAAACAGTTTGAGTGAGAAGTTTTCTGAGGCGGAAAACCTATTGGAAATTGTGAGGGATGATTTAAATCTCACACAAGTTAAGTTGCAAAGCACTGAAAATGATCTCAAGGCTGCTGAATTAAGAGAGAGCGAGATAACAAGAAAAAATTATGCTACTAGAAAGAAATTCTTTAGG
TARGET=157,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4484
PRIMER_LEFT_PENALTY=0.415225
PRIMER_RIGHT_PENALTY=0.033137
PRIMER_LEFT_SEQUENCE=TACAGTCAACGCAGCCTAGAAC
PRIMER_RIGHT_SEQUENCE=TGTACTTTCGAGGGTTGAGGTT
PRIMER_LEFT=83,22
PRIMER_RIGHT=346,22
PRIMER_LEFT_TM=59.585
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.4949
PRIMER_LEFT_1_PENALTY=0.415225
PRIMER_RIGHT_1_PENALTY=0.079714
PRIMER_LEFT_1_SEQUENCE=TACAGTCAACGCAGCCTAGAAC
PRIMER_RIGHT_1_SEQUENCE=TCGAGCTTGAAACACATCAAGT
PRIMER_LEFT_1=83,22
PRIMER_RIGHT_1=325,22
PRIMER_LEFT_1_TM=59.585
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.5712
PRIMER_LEFT_2_PENALTY=0.538083
PRIMER_RIGHT_2_PENALTY=0.033137
PRIMER_LEFT_2_SEQUENCE=TGTTCTGAGCATGAAGATCGAG
PRIMER_RIGHT_2_SEQUENCE=TGTACTTTCGAGGGTTGAGGTT
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=346,22
PRIMER_LEFT_2_TM=60.538
PRIMER_RIGHT_2_TM=60.033
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=294
PRIMER_PAIR_PENALTY_3=0.6178
PRIMER_LEFT_3_PENALTY=0.538083
PRIMER_RIGHT_3_PENALTY=0.079714
PRIMER_LEFT_3_SEQUENCE=TGTTCTGAGCATGAAGATCGAG
PRIMER_RIGHT_3_SEQUENCE=TCGAGCTTGAAACACATCAAGT
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=325,22
PRIMER_LEFT_3_TM=60.538
PRIMER_RIGHT_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.6931
PRIMER_LEFT_4_PENALTY=0.659953
PRIMER_RIGHT_4_PENALTY=0.033137
PRIMER_LEFT_4_SEQUENCE=AATGTTCTGAGCATGAAGATCG
PRIMER_RIGHT_4_SEQUENCE=TGTACTTTCGAGGGTTGAGGTT
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=59.340
PRIMER_RIGHT_4_TM=60.033
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_01800 Ps_contig_mira-and-tgicl-ass_franssen_16548
SEQUENCE=CTAAAGACACGTTCTTGAAAAACTAGAGAGAAATTAGCWAAAAGACTTTTCTCTGTTAAAACCACAAACAAAAGAACAAGGCATAGGTTTTTCGACAGATTCAACAGGATAACCAAATATTTCTCAACTCAATCAAAGATTTTTTCTTTGTTAAACCTAAACCAAATAGAAGAAACTTTTTTTTTCCAGAGAACAATCAAACAATAATCCATAAAAACCTCTAAATCCTAAGACATCAAGCCAAGCAACAACAACACTCAAAGCAAAAATAAAAGAAAGCGAAAAATAGAAGAAGATGGAGAACTTATCTTAAACCGTGGGGAGCTTCGCCGGAGTTGGATTTCAAACAAGTAACTTTTTCCTCCTTCGTTAAAGTCTTTGCATCATCATCATTTACGCCTCTTCATACTGGATGCGATCTTTCTTTTAATTTCTTCAAAACTTTGCTGTAGAACTTCAGATCTTGAATCTTAGGGGTTTAGGTTTTGGGTTGTTTTAAGTTTTTTGGTTTAAGGTTAAGAACGAATA
TARGET=382,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01801 Ps_contig_mira-and-tgicl-ass_franssen_16549
SEQUENCE=TCAAGCCAAGCAACAACAACACTACAAAGACAAAAAATAAAAGAAAGACGAAAAATAGAAGAAGATGGAGAACTTATCTTAAACCGGTGGGGAGCTTCGCCGGAGTTGGATTTCAAACAAGTTAACTTTTTCCTCCTCCGTTAAAGTCTTTGCATCATCATCATTTACGCCTCTTCATACTGGATGCGATCTTTCTTTTTAATTTCTTCAAAACTTTGCTGTAGAACTTCAGATCTTGAATCTAGGGTTAGGTTTTGGGTGTTTAAGTTTTGGTTAAGGTTAAGAACGAATCAAGCTACGTTTGGCCATATGAGAAGGAAAGTTTAGGGTTAGTTTTGGTATTTCAAGGGAGGGCCTCGATCCGGCCATTTGAGGAACAAATCGGAAAAAGCGAGTTCATATTTGAACTTAGAGGCAAAAATAAAGGTGAGGTTTTCTTTTTTGTTAGGGTTGAGGGCGGTGGTCACCGGAGAAGACGGTGGCGGCCGCCGCGCGCCGCCGCTTCTCCGATGAAGGTTGAACGGAGAAGATGAAAGGAGTTGCAGAGCAACTCTGGTTATTCTTGAAGAGAGAGAATTATTTTTAACAAATGAAGCGCTTCC
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4945
PRIMER_LEFT_PENALTY=0.384647
PRIMER_RIGHT_PENALTY=0.109882
PRIMER_LEFT_SEQUENCE=CGGAGTTGGATTTCAAACAAGT
PRIMER_RIGHT_SEQUENCE=ATATGGCCAAACGTAGCTTGAT
PRIMER_LEFT=100,22
PRIMER_RIGHT=310,22
PRIMER_LEFT_TM=60.385
PRIMER_RIGHT_TM=59.890
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=0.5562
PRIMER_LEFT_1_PENALTY=0.446290
PRIMER_RIGHT_1_PENALTY=0.109882
PRIMER_LEFT_1_SEQUENCE=AACTTATCTTAAACCGGTGGGG
PRIMER_RIGHT_1_SEQUENCE=ATATGGCCAAACGTAGCTTGAT
PRIMER_LEFT_1=70,22
PRIMER_RIGHT_1=310,22
PRIMER_LEFT_1_TM=60.446
PRIMER_RIGHT_1_TM=59.890
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.8931
PRIMER_LEFT_2_PENALTY=0.384647
PRIMER_RIGHT_2_PENALTY=0.508459
PRIMER_LEFT_2_SEQUENCE=CGGAGTTGGATTTCAAACAAGT
PRIMER_RIGHT_2_SEQUENCE=CTCATATGGCCAAACGTAGCTT
PRIMER_LEFT_2=100,22
PRIMER_RIGHT_2=313,22
PRIMER_LEFT_2_TM=60.385
PRIMER_RIGHT_2_TM=60.508
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.9547
PRIMER_LEFT_3_PENALTY=0.446290
PRIMER_RIGHT_3_PENALTY=0.508459
PRIMER_LEFT_3_SEQUENCE=AACTTATCTTAAACCGGTGGGG
PRIMER_RIGHT_3_SEQUENCE=CTCATATGGCCAAACGTAGCTT
PRIMER_LEFT_3=70,22
PRIMER_RIGHT_3=313,22
PRIMER_LEFT_3_TM=60.446
PRIMER_RIGHT_3_TM=60.508
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.9642
PRIMER_LEFT_4_PENALTY=0.149895
PRIMER_RIGHT_4_PENALTY=0.814339
PRIMER_LEFT_4_SEQUENCE=AAGCCAAGCAACAACAACACTA
PRIMER_RIGHT_4_SEQUENCE=GAAAGATCGCATCCAGTATGAA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=194,22
PRIMER_LEFT_4_TM=59.850
PRIMER_RIGHT_4_TM=59.186
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_01802 Ps_contig_mira-and-tgicl-ass_franssen_16557
SEQUENCE=AACTTGCTTCAATAATTTCCAACTACTAAGATTTCTTTGTTCTGTTACATACATACACACATAAACTACAAACCAAAGCGTCAAATAATAACCTATATATTTGGATTACACCTA
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01803 Ps_contig_mira-and-tgicl-ass_franssen_16558
SEQUENCE=AACTTGCTTCAATAATTTCCAACTACTAAGATCTGTTCTGTTACATACATACACACATAAACTCAAACCAAAGCGTCAAATAATAACCTATATATTTGGATTACACCTA
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01804 Ps_contig_mira-and-tgicl-ass_franssen_16559
SEQUENCE=AGTTAAACAATTTTTGGGAAAATGTCATTCCTTCATTTGGTGCAAATGTCGTTTTGCTAAAATTTCCAAACATTAATGTTACCAGCCCAGCATATTGTGGTTCAATAGCCAAAACGAGACTAGAGAAAAGACTTGGCTTTTCCCAGTAAACAATGCTTCAACTACGGATACATACATATACCAAAGAAGGGCATAAAAAAGAAACCTATGTCTTAAACTTCATAAAAACTATCCATCAACTTCATCTAAGAAACAAAAGCATATTAACCAGATCAGTAAAGACTCCAACATAGTTTGTCTCTGAATGAAATTTTGCTAGCGAGCTCTCTTGTTACTACTGCATGAGGGGCACTTGTATTGCTTGATATGCTCGGCCCGTGCTGGAGTGATTTTCACACATTTGCCATGGAACCACTTCTCACAAATATCACAGCAAATCCAGAACTCATCAGTGCCATCATTATCACCACAAGCCCCACACATGGCTTCTCCATGTTCATCATCATCATTCTCTTCCAGTCCTTCCTCCTCATCTTTTGGTTGTAATGCCTTTGGTAGTCTGCTCTGAGTTTCAGGAGCCCGCACTTTAGAACTGGACTTAGATTTGCTGCCACTGTTGTTTGAGACTGAGGACTTCTCTTTGGCTGGTTTCTTGGTTGAGCCATTAACAATTTCAAATATTGTTGGTAGTTCATTAATCATATTGAATAGACGTTTCCTGTCAGTTCTGTCAAACGCAAATCTAGCTCCAAAGTAGAATGCAAGGGAAAGCAACCATGTATCACTATGAACAGCAACTAAGGATAACCAGTCTTTTTCTTGCATGCCATCCCTAGCAAAGTTGATACCCAGCACAGGCTCAGGAAGCTCTGGAGGAACTTCCTCAACAGGTAAATTCACTTCCCACTTCTCATTAAGTAATCCATATAAGCACAAATTCTCTTTCTCAGGATCACATTGTCTGTAGAAATCTTCAACATCAACAGTTAGGGCTTTGATGATTCCAGCTCTTCGGCTTTTGAAATCCTCGAAAATATCTTCCACAGAGCGACCTCGACCTTCCATTTGATTCAATGAGAAATCGTTATGTTTATGGTTAAGGGTTTCGTTACAGTAAAACGCAAAGAGAGAATGAAACGATGTCGTTTTAGGGTTTTTTTTAGTTCACGTTCTCTCGCAACGCAAGAGATGAGACTCACACTAACATAAAGACGAAAACGTTTTTATATAATTATTTGAGAAAAACTGAGACAAACTCAGAGAACAAAGGAAAAACACAACACAACGCAGTTTCTTCTTCCYTCGAGTCGGTGCGTGTGGATTTTATTTTACGGGCGGGGATTCTCTGTGCTCGACTCC
TARGET=497,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01805 Ps_contig_mira-and-tgicl-ass_franssen_16571
SEQUENCE=TAATAAAATATAGGACTGCAAATGCTAACTTCCACCCACCCACTAATTTTTTTACTTTGTCTAAATTAACAAGTTACACCAATGTGCATTTAAATTTTTTTGTAATAACTTATTAATTTACATACAGTGAATGTAATTTAATAAACTCATATAAGATTATAGAAGGATTTTGTGAACAATAAATAAAGTTTGATATTGATCAAGGATTGTTTCATCAAGGTATCTAACTTACTCTTTCTTCTACCATATTCTTAGTCTAGTATCTCAGTCACATTCTTCACATGAAAGCTCTTTGGATTTGAAAAATTATAGTAAATGCATGAAAAACTATCCATAAGACACATGAATACTTGGAGATATATTACGTTGATTCAACAGAACAATAGTATGGTTAGCTGTGTAATTAACTTAAGAATTAATCTAGTTAAATTGACTGTAACTTGATGTGTAAAAAAGAAAGGGAATGTATAGAGTTCATAAAGAAGTAATTAAAAGAAATACTGAAAAGAAAGCAGAAAGATGTTTTCTTTTATAAAGAAAAGATAGGAATAAAATAGAATCTAATTCTACAAAATTATATGCTTCAACTACTATCCTATCCTCCTCCATCAATGAATGTATTCCACTCAAAACAAACTTATCTATTAATTACTGAAAGTGATCCAAAGCT
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01806 Ps_contig_mira-and-tgicl-ass_franssen_16572
SEQUENCE=AAAATTGAGCAAGTTATAGTCTAGCTAGCCAATTTCACGTCCCTTTTATTACAAACAATTATTGAATCTGGCCTGCGATAACCTGCAAATATTCACGGCCTTCCACGTAGCACAGTGCCAATTGTAATAAAATATAGGACTGCAAATGCTAACTTCCACCCACCCACTTAATTTTTTACTTGTCTAAATTAACAAGTTACACCAATGTGCATTTTAAAATTTTTTGTAATAACTTTAA
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01807 Ps_contig_mira-and-tgicl-ass_franssen_16575
SEQUENCE=CGAAAGACCACCCACTCTTCACCGACATACAGTATGTGGCCACCATTTTCTTGTAATAAACGGACCGTTTAAACTTAGATGATAGGTCGTCAGAGCCACTTCGCAGTGCTTCCTCAAAATCTACTTGCTCCAATTGGAAGAATCATGATTTCCTCAATGGCCAGACTATTTCCAGCATGTTTTCCGCCGCTTATAACAGTAAAAGCAGGAACAGGAAGCATTGGGGTAGCTTTTCCAGACAGGTCAGCGATGTGCTTGTACAGTGGAACTTCCTTTTCAGCGGCCCCAGCTCTACATGCAGCAATGGACACGGCTAATATAGCATTTGCTCCAAGTTCCCCCTTCTTCTCTGTTTTGTCCAGGTCTATCATAGCATGATCAATCTGTGACTGCAGGGTTGGATCCATACCAATCAGCGCCTCAGAAATTTTTTCATTTATATTTTTAACAGCTTTAGCAACACCGTTTCCTAGATATACACCTTTGTCACCATCCCGTAACTCAACAGCCTCATACATGCCAGAGGAGTTGCCGGAGGGAACAGAAGCACGGAACATAGCTTTGTTAGTATACAAATCGACTTCAACAGTTGGAATTCCTCTACTATCAAGAATTTGTCTCGCTTTCACTTTGGTGATTACCGATTGTACTCCTTTTTTCATATGATTCGAGATGAAGAGAACGGGATCGGTGGTTTTGGCTCTGACGGCGGCATTGACGGAATCTTCGAGTTTACGGGAAAGCGTGTATTTGTCGAGATATTCTTGCACTGACATTTCTTCAACTAACTTCTTCTTCTTCTTTCACGATCTCGTGATTGAAATGAGCTCAATGTTGGTTGTTATTGTTGCTATTGATATTGCTTGCTTTCTTCAACTGAGATTCAGTGCTGCCAGCTTTGTGTCTACGAACC
TARGET=789,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6280
PRIMER_LEFT_PENALTY=0.434710
PRIMER_RIGHT_PENALTY=0.193337
PRIMER_LEFT_SEQUENCE=GAGTTTACGGGAAAGCGTGTAT
PRIMER_RIGHT_SEQUENCE=CTCAGTTGAAGAAAGCAAGCAA
PRIMER_LEFT=728,22
PRIMER_RIGHT=880,22
PRIMER_LEFT_TM=59.565
PRIMER_RIGHT_TM=59.807
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.7091
PRIMER_LEFT_1_PENALTY=0.322785
PRIMER_RIGHT_1_PENALTY=0.386286
PRIMER_LEFT_1_SEQUENCE=ACAGAAGCACGGAACATAGCTT
PRIMER_RIGHT_1_SEQUENCE=TTGAGCTCATTTCAATCACGAG
PRIMER_LEFT_1=540,22
PRIMER_RIGHT_1=831,22
PRIMER_LEFT_1_TM=60.323
PRIMER_RIGHT_1_TM=60.386
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.7361
PRIMER_LEFT_2_PENALTY=0.542749
PRIMER_RIGHT_2_PENALTY=0.193337
PRIMER_LEFT_2_SEQUENCE=GTTTACGGGAAAGCGTGTATTT
PRIMER_RIGHT_2_SEQUENCE=CTCAGTTGAAGAAAGCAAGCAA
PRIMER_LEFT_2=730,22
PRIMER_RIGHT_2=880,22
PRIMER_LEFT_2_TM=59.457
PRIMER_RIGHT_2_TM=59.807
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=151
PRIMER_PAIR_PENALTY_3=0.7396
PRIMER_LEFT_3_PENALTY=0.434710
PRIMER_RIGHT_3_PENALTY=0.304841
PRIMER_LEFT_3_SEQUENCE=GAGTTTACGGGAAAGCGTGTAT
PRIMER_RIGHT_3_SEQUENCE=TCTCAGTTGAAGAAAGCAAGCA
PRIMER_LEFT_3=728,22
PRIMER_RIGHT_3=881,22
PRIMER_LEFT_3_TM=59.565
PRIMER_RIGHT_3_TM=60.305
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.7914
PRIMER_LEFT_4_PENALTY=0.598109
PRIMER_RIGHT_4_PENALTY=0.193337
PRIMER_LEFT_4_SEQUENCE=CTCGCTTTCACTTTGGTGATTA
PRIMER_RIGHT_4_SEQUENCE=CTCAGTTGAAGAAAGCAAGCAA
PRIMER_LEFT_4=618,22
PRIMER_RIGHT_4=880,22
PRIMER_LEFT_4_TM=59.402
PRIMER_RIGHT_4_TM=59.807
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_01808 Ps_contig_mira-and-tgicl-ass_franssen_16576
SEQUENCE=CCAACTTGGAGATGAAATGAGAAAGAAAGAAGGAAAGCTTGACTCCACAGGTTTGACTTCTCAAAACATTAAGCTTATATAAACTTTATACAAAGAACAAATTTCATATTGT
TARGET=20,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01809 Ps_contig_mira-and-tgicl-ass_franssen_16577
SEQUENCE=RCATAATTCCTCAGGATCCAACAATGTTTGAAGGGACTGTAAGAACTAACCTCGACCCGCTAGAAGAGTACACAGATGAACAAATTTGGGGAGGCTCTTGATATGTGCCAACTTGGAGATGAAATGAGAAAGAAAGAAGGAAAGCTTGACTCCACAGGTTTGACTTCTACAAAACATTAAGCTTATAATTAAACTTTATAACAAAGAACTAAATTTCATTATTGTTCTTTCTTATTTATAATATAATATTATTTATTTACTTACCCCTTAAATAAATACTAATTGTTTCTTTTTTTTA
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01810 Ps_contig_mira-and-tgicl-ass_franssen_16579
SEQUENCE=TTTATTCAGAAATGTTTGAATCGGCGTGTCCAAAATCTTATAGCTACGCTTATGATGATGCAACTAGCACTTTTACTTGTTCTGGTGCTGATTATACAATCACATTTTGTCCTTCTTCTACACCTAGTCTAAAGTCAGCAACGGATTCGGATTCTAATAATACGAAGGAGACGGGTAATTCGGCTTCGGGGAGTGGCTCGGAGCAATCGGAACTGGCCTCAGCTTCGTGGCTGGCTTATATGGCTACGGCTTCTGAGGCTTCTATGAGGAGCAGAACCTCAATGGTTTCTTTGTTGTTGTTGTTGATTGGTTTTATTTTTAATGTTTTTGTGTTTCATTGATTTTAGTTTGTATGCTTATCTTACTTTTTAAGAGAATATAAATAGAAATATACGGTTGGTAAAATTGTTCAATTAGTGTGTAGTGTTAAAACATTGTTGGTTAAATTGTATATAAAGTAATAAGCAATTCAATACTAAA
TARGET=291,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01811 Ps_contig_mira-and-tgicl-ass_franssen_16588
SEQUENCE=CACGGTTTTGATTGGGTTGTTAATTGCATGGGAATTCAGAAGCAAATGGGTGAGAATTGTGAAGAAACATAAAGAGTGTATAGGAACTTTTTCGGGTTTTGAATTCGAGTACCCATTTTCTCAGAGAGAGAGAGAGAGAGAKTTGATTCTGTC
TARGET=123,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01812 Ps_contig_mira-and-tgicl-ass_franssen_16613
SEQUENCE=GGGTAGGCGAGCCGGTTCCGAATAATTCTAGCCGTGGTTCGTTGCTATCCTATATCGCATTGCACGTGCCAGACAGCGAAGAACTTGCTCGGGTTGAGGTTCTTGGAGTTCTTGAGTGGGCATCCAAGCAACTTAACGTGACTCAAGATGAAACCGTCGCCATGTTGTTGCAAGAGTCCAAGAGTAGGCTTGAGCTTTACCAGTCTAGAGGTTCAAGAGGGTTCCAAAAGTTACATCAATAGGATGAGATTAGTTCTTATTGTTGTTGTTGGTGTGTGAGGTTTTGAATTGATTTGTATCTGGTATACTGTTCTTGTGAAGNGTGTA
TARGET=260,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.1861
PRIMER_LEFT_PENALTY=0.102490
PRIMER_RIGHT_PENALTY=3.083617
PRIMER_LEFT_SEQUENCE=TAGAGGTTCAAGAGGGTTCCAA
PRIMER_RIGHT_SEQUENCE=CACNCTTCACAAGAACAGTATACCA
PRIMER_LEFT=205,22
PRIMER_RIGHT=324,25
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=59.916
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=3.2294
PRIMER_LEFT_1_PENALTY=0.145769
PRIMER_RIGHT_1_PENALTY=3.083617
PRIMER_LEFT_1_SEQUENCE=GTTCTTGGAGTTCTTGAGTGGG
PRIMER_RIGHT_1_SEQUENCE=CACNCTTCACAAGAACAGTATACCA
PRIMER_LEFT_1=98,22
PRIMER_RIGHT_1=324,25
PRIMER_LEFT_1_TM=60.146
PRIMER_RIGHT_1_TM=59.916
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=227
PRIMER_PAIR_PENALTY_2=3.2294
PRIMER_LEFT_2_PENALTY=0.145769
PRIMER_RIGHT_2_PENALTY=3.083617
PRIMER_LEFT_2_SEQUENCE=GGTTCTTGGAGTTCTTGAGTGG
PRIMER_RIGHT_2_SEQUENCE=CACNCTTCACAAGAACAGTATACCA
PRIMER_LEFT_2=97,22
PRIMER_RIGHT_2=324,25
PRIMER_LEFT_2_TM=60.146
PRIMER_RIGHT_2_TM=59.916
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=228
PRIMER_PAIR_PENALTY_3=3.3035
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=3.083617
PRIMER_LEFT_3_SEQUENCE=ATGTTGTTGCAAGAGTCCAAGA
PRIMER_RIGHT_3_SEQUENCE=CACNCTTCACAAGAACAGTATACCA
PRIMER_LEFT_3=161,22
PRIMER_RIGHT_3=324,25
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=59.916
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=7.75
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=3.3382
PRIMER_LEFT_4_PENALTY=0.254567
PRIMER_RIGHT_4_PENALTY=3.083617
PRIMER_LEFT_4_SEQUENCE=CTAGAGGTTCAAGAGGGTTCCA
PRIMER_RIGHT_4_SEQUENCE=CACNCTTCACAAGAACAGTATACCA
PRIMER_LEFT_4=204,22
PRIMER_RIGHT_4=324,25
PRIMER_LEFT_4_TM=59.745
PRIMER_RIGHT_4_TM=59.916
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_01813 Ps_contig_mira-and-tgicl-ass_franssen_16626
SEQUENCE=AAGTTTTTTTTAAAACCTTAAGAGTAAATTWAATTWCAATCCCCCTAAAAAACTTAAACAAGTGTCATTACTTCTACAATGTTTTCATTCCGAAATAGACAAGAACATAGATTAACCTTACAGAAAAAAAGGAAAGGAAAAAAAGAAAACMAAGAACAAAACATCCTAGTTAAAATTAACGGTTACCAACTGTGTCTAAGGGTGTTTGTGTTGTTGATGCCTAACACTGTGTTGGTGTTGATGTTGATGTGGATGACTCTTGCTATTACCACCAGATGTAGATTCTCTTTTCTGCGGGGAAGAAAAAAGCTGCCCAAACCCGAAAACCGAACCGGATTTTGAACCGCCTCTTAATGAACAAACCGGAACATTGAGAACCGGAGTGACCCGGACATTAGCTGAATAAGACCGTTCCATTCCACGATGCTTAAAGCGGGGCCCGCCGGTGAAACTCCCGGGACTACTCGAGCTACGTTCTAAGCTCTGGAAAACGATTTTCCCTGATGAGTTTAATCTAGGGCTATCCACGGAAGCTCCCCTGCACCCTTGCGTACTAGATGATTCCATTCGGATCGGACTTCTCCCGGAGCGGTTATTGTTGTTGTGCTGATCCGGTTTGTTTCCATCGAAGGAGCCTGCTCGGGGTTTGACTAACCGGATCCTTGGATTCGAGTTCGGGTTTGGGTTACGGTGGATGGAAATCGGGTTAGGTTTTTCTGAATCGAGAGAGAGTCGTGGGAGATCGTCGTGGTCGTGGCTTGAAGATGAGGAGGAAAGCGAGAGACGAGAAGAGATCGAGAGTGATTCGTTGTCGGAGTCTCTCAATAGAGGAAGACTGAGAGAACTGTTGTCTGATGACGACGTCGTTTTGGAGTTACGGTTGAGGAGGTGTTTGAACGATTTGTTGGAAGGAGAAGAGGATGAAGTTGCCGGCGATGTTTTGGTGGTTGTTGTTGTTGTGTTGGTGGAGCTGTAGAGTTTTTTGKAGGCCGAGAAGGTCTTTCCAGCGGCTAGAGCAGCGTGGTGCTTTAGGAGAGAAGAGAATGGGGTCGGAGGTTGTTATGGAGGCGGTAGTTTTCTTGGGAGAAGTGGTGGCGGAGATGGAGGTGGTTGGTTTGAAGTGAAGAGGGACGAGTTTACCGTCGGAGAAGAGTTGGTCGGCGGGAAGCATGGTAACGGGATCTTGAAGACGGAACTCAAAGTCGGGTTCGGGATCCCATGAGAAGAAGGGTTCCGGTGACATTCCGATGTTGTTGATGCAGGCAGAAGCCATTGGCGGATCATGCACTAGCTAACTAAGTGAATACCTGGAAATGTTGTGTCTGAGTTTATTGAAGCAAAAAATACAGAGAGATAGAGAGAGTAATAGATAGATATATAGATATAATAGTTATATTGAGGTAGTGGTGGATGATGTTATGTTTGATTGATGAGAAGAGAAAAGATGGAAGAGGTGGTTGTTGTTGTTACTTGTAACCGGTACGGTGGGATATATATTATGGAATAGAGTAGAGTAGATTGAGAGTGGGCGAAGGAGGCACATATATAACAGTTTTTGCAGCTACTCGCATCGCTTCACTAGACTCTAGGGCCACCTCGCTCCCTTTTCCCCTCCCYCRACCCTACTCTCTCTCTCTTTTAAAAAGACACAATCTCC
TARGET=947,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01814 Ps_contig_mira-and-tgicl-ass_franssen_16626
SEQUENCE=AAGTTTTTTTTAAAACCTTAAGAGTAAATTWAATTWCAATCCCCCTAAAAAACTTAAACAAGTGTCATTACTTCTACAATGTTTTCATTCCGAAATAGACAAGAACATAGATTAACCTTACAGAAAAAAAGGAAAGGAAAAAAAGAAAACMAAGAACAAAACATCCTAGTTAAAATTAACGGTTACCAACTGTGTCTAAGGGTGTTTGTGTTGTTGATGCCTAACACTGTGTTGGTGTTGATGTTGATGTGGATGACTCTTGCTATTACCACCAGATGTAGATTCTCTTTTCTGCGGGGAAGAAAAAAGCTGCCCAAACCCGAAAACCGAACCGGATTTTGAACCGCCTCTTAATGAACAAACCGGAACATTGAGAACCGGAGTGACCCGGACATTAGCTGAATAAGACCGTTCCATTCCACGATGCTTAAAGCGGGGCCCGCCGGTGAAACTCCCGGGACTACTCGAGCTACGTTCTAAGCTCTGGAAAACGATTTTCCCTGATGAGTTTAATCTAGGGCTATCCACGGAAGCTCCCCTGCACCCTTGCGTACTAGATGATTCCATTCGGATCGGACTTCTCCCGGAGCGGTTATTGTTGTTGTGCTGATCCGGTTTGTTTCCATCGAAGGAGCCTGCTCGGGGTTTGACTAACCGGATCCTTGGATTCGAGTTCGGGTTTGGGTTACGGTGGATGGAAATCGGGTTAGGTTTTTCTGAATCGAGAGAGAGTCGTGGGAGATCGTCGTGGTCGTGGCTTGAAGATGAGGAGGAAAGCGAGAGACGAGAAGAGATCGAGAGTGATTCGTTGTCGGAGTCTCTCAATAGAGGAAGACTGAGAGAACTGTTGTCTGATGACGACGTCGTTTTGGAGTTACGGTTGAGGAGGTGTTTGAACGATTTGTTGGAAGGAGAAGAGGATGAAGTTGCCGGCGATGTTTTGGTGGTTGTTGTTGTTGTGTTGGTGGAGCTGTAGAGTTTTTTGKAGGCCGAGAAGGTCTTTCCAGCGGCTAGAGCAGCGTGGTGCTTTAGGAGAGAAGAGAATGGGGTCGGAGGTTGTTATGGAGGCGGTAGTTTTCTTGGGAGAAGTGGTGGCGGAGATGGAGGTGGTTGGTTTGAAGTGAAGAGGGACGAGTTTACCGTCGGAGAAGAGTTGGTCGGCGGGAAGCATGGTAACGGGATCTTGAAGACGGAACTCAAAGTCGGGTTCGGGATCCCATGAGAAGAAGGGTTCCGGTGACATTCCGATGTTGTTGATGCAGGCAGAAGCCATTGGCGGATCATGCACTAGCTAACTAAGTGAATACCTGGAAATGTTGTGTCTGAGTTTATTGAAGCAAAAAATACAGAGAGATAGAGAGAGTAATAGATAGATATATAGATATAATAGTTATATTGAGGTAGTGGTGGATGATGTTATGTTTGATTGATGAGAAGAGAAAAGATGGAAGAGGTGGTTGTTGTTGTTACTTGTAACCGGTACGGTGGGATATATATTATGGAATAGAGTAGAGTAGATTGAGAGTGGGCGAAGGAGGCACATATATAACAGTTTTTGCAGCTACTCGCATCGCTTCACTAGACTCTAGGGCCACCTCGCTCCCTTTTCCCCTCCCYCRACCCTACTCTCTCTCTCTTTTAAAAAGACACAATCTCC
TARGET=1457,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01815 Ps_contig_mira-and-tgicl-ass_franssen_16626
SEQUENCE=AAGTTTTTTTTAAAACCTTAAGAGTAAATTWAATTWCAATCCCCCTAAAAAACTTAAACAAGTGTCATTACTTCTACAATGTTTTCATTCCGAAATAGACAAGAACATAGATTAACCTTACAGAAAAAAAGGAAAGGAAAAAAAGAAAACMAAGAACAAAACATCCTAGTTAAAATTAACGGTTACCAACTGTGTCTAAGGGTGTTTGTGTTGTTGATGCCTAACACTGTGTTGGTGTTGATGTTGATGTGGATGACTCTTGCTATTACCACCAGATGTAGATTCTCTTTTCTGCGGGGAAGAAAAAAGCTGCCCAAACCCGAAAACCGAACCGGATTTTGAACCGCCTCTTAATGAACAAACCGGAACATTGAGAACCGGAGTGACCCGGACATTAGCTGAATAAGACCGTTCCATTCCACGATGCTTAAAGCGGGGCCCGCCGGTGAAACTCCCGGGACTACTCGAGCTACGTTCTAAGCTCTGGAAAACGATTTTCCCTGATGAGTTTAATCTAGGGCTATCCACGGAAGCTCCCCTGCACCCTTGCGTACTAGATGATTCCATTCGGATCGGACTTCTCCCGGAGCGGTTATTGTTGTTGTGCTGATCCGGTTTGTTTCCATCGAAGGAGCCTGCTCGGGGTTTGACTAACCGGATCCTTGGATTCGAGTTCGGGTTTGGGTTACGGTGGATGGAAATCGGGTTAGGTTTTTCTGAATCGAGAGAGAGTCGTGGGAGATCGTCGTGGTCGTGGCTTGAAGATGAGGAGGAAAGCGAGAGACGAGAAGAGATCGAGAGTGATTCGTTGTCGGAGTCTCTCAATAGAGGAAGACTGAGAGAACTGTTGTCTGATGACGACGTCGTTTTGGAGTTACGGTTGAGGAGGTGTTTGAACGATTTGTTGGAAGGAGAAGAGGATGAAGTTGCCGGCGATGTTTTGGTGGTTGTTGTTGTTGTGTTGGTGGAGCTGTAGAGTTTTTTGKAGGCCGAGAAGGTCTTTCCAGCGGCTAGAGCAGCGTGGTGCTTTAGGAGAGAAGAGAATGGGGTCGGAGGTTGTTATGGAGGCGGTAGTTTTCTTGGGAGAAGTGGTGGCGGAGATGGAGGTGGTTGGTTTGAAGTGAAGAGGGACGAGTTTACCGTCGGAGAAGAGTTGGTCGGCGGGAAGCATGGTAACGGGATCTTGAAGACGGAACTCAAAGTCGGGTTCGGGATCCCATGAGAAGAAGGGTTCCGGTGACATTCCGATGTTGTTGATGCAGGCAGAAGCCATTGGCGGATCATGCACTAGCTAACTAAGTGAATACCTGGAAATGTTGTGTCTGAGTTTATTGAAGCAAAAAATACAGAGAGATAGAGAGAGTAATAGATAGATATATAGATATAATAGTTATATTGAGGTAGTGGTGGATGATGTTATGTTTGATTGATGAGAAGAGAAAAGATGGAAGAGGTGGTTGTTGTTGTTACTTGTAACCGGTACGGTGGGATATATATTATGGAATAGAGTAGAGTAGATTGAGAGTGGGCGAAGGAGGCACATATATAACAGTTTTTGCAGCTACTCGCATCGCTTCACTAGACTCTAGGGCCACCTCGCTCCCTTTTCCCCTCCCYCRACCCTACTCTCTCTCTCTTTTAAAAAGACACAATCTCC
TARGET=1626,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01816 Ps_contig_mira-and-tgicl-ass_franssen_16661
SEQUENCE=ATSCASTTGCMGCMASWGMAGCCGCCGCCGCCTCTGCGTTTGCAGGAAGTACATCCCTCTCCTCCTGCGTGATACAAATTCAACACACAACGCAACAAGAAATAAAACCATGTCACAAACTAAAACTCACCCACATAACTTACCAAATAAACATAGGGTCAGTTTGGGAGAGCTAACATAAACAGCTCATGATCATGACATGGCCATAAACTATTTCTATTTTCAACTTATTTTCATAAGCTCTCCAAGACAACTTAAGAAAACAACAACATATAACTTATATAAGAAACCGTTTATAC
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01817 Ps_contig_mira-and-tgicl-ass_franssen_16676
SEQUENCE=GTCGGGGACCTCCTSGCTCTTCYCAAATTSAACTACGCTTTCTTTCCCCTCCTTATATCTCAACTACTTTATCCTCTTCATCAAAATTCAAAATCCCTATTCAATTTTTGACCTAATTTCTTTCAATCCAACCAATTTCACGAATGAAATTCATCAATCCCTATTTCCTTAGGTGAGATTCAAATCCACAATTGCGGTTTGAATTTGAATTGATTGATGTCTGGTTCTTCTGATGAAGAGATCTCGAACGGTAATCGCGATGGTTACCAAAGAGGAGGAAGTGACGGCGATGACGATGAAGATGAAGAAGAGTATCGGAAGAAGAAGAATCGTGGAAGAGTTGATTTTGGTTTTGGTAAGAGGTTGGTACTTCGTCAATTTACAAAAGCTAAGAATCAAATTCGGAGAGTCAGAAGCAGAAAGGCTCTTCTTTCGTCTTCGTCGTCGTTAACAGCGGCTTCGTCGAGGAATGTGAATGTGAGTAGTAGAGGTAAGGTGGTGATTATTCACGGTAATGATACTGATAGTAGTATAATACTGCTACTACGAAATAGGAATTTAGGTTAGTTAAGTTTTNTTTTCGCGTCCTAGTGTATT
TARGET=318,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01818 Ps_contig_mira-and-tgicl-ass_franssen_16688
SEQUENCE=GGATTGCRKKKAGGGATGGAAAATCTGAAACCCTAAACCCTGACCTGTGTGAGTGATAGAGCCGATTCAATGACAAACAATCCAAAGCACACAGTTGAATTTGAAGAATGCAGTGATAATAGGCCGTCAAAGATTTCAAAAATCGAGTATCCCAATCCCATCGTCGACGGCGAAGAAGAAGAAGCAAAGACAGTGATTGAAGACCAAAAGCAAGAGATGAACCAACACCGTAAGATTCAACGCTACTTGGTGGCGATTGAGTACATCGGAACTCGATTCTGTGGTTCTCAGAAGCAGCTCACTGACCGTACTGTTGTTGGTGTTCTTGAGGAAGCTTTTCGTAAATTTGTTGGACAACGAGT
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01819 Ps_contig_mira-and-tgicl-ass_franssen_16696
SEQUENCE=TCGGGTACTACGAATTTGTTTAGGACATTGAAGCTAAAGAAAACCTCATCTCTTCACCTCACCACTCGTCCCTGCAACACCACCACTTCAAACTTCAAACCCAGTTTTTTTCCTCTTTTCTTTAGCAATGGCTTATATAAAATCTTGTATTTCTTATTCTGCTTTACCCTTCTTCCAACGGAGGGGTCTACCACCTGCCTCTTCACCTTTTCATCACACAGGAACATTCATTCCCCAATGTTTAACACCAAAAATATTAAGTGCCAAGTTTTCAAAGAATGTCAGATTCTCTATTCCTAGAAGTACTACTTCTTCTTCTTCAAACTCCACTGATGATGATGACACCATTAATCAGACTAAGACTAAGAAAACACCATTTGGATATTCAAAGGAAAAGATGTTATATTAATTGGACTTGGTGTTACTTT
TARGET=309,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4399
PRIMER_LEFT_PENALTY=0.045112
PRIMER_RIGHT_PENALTY=0.394838
PRIMER_LEFT_SEQUENCE=CACCACCACTTCAAACTTCAAA
PRIMER_RIGHT_SEQUENCE=GGTGTCATCATCATCAGTGGAG
PRIMER_LEFT=77,22
PRIMER_RIGHT=345,22
PRIMER_LEFT_TM=60.045
PRIMER_RIGHT_TM=60.395
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=269
PRIMER_PAIR_PENALTY_1=0.6243
PRIMER_LEFT_1_PENALTY=0.229480
PRIMER_RIGHT_1_PENALTY=0.394838
PRIMER_LEFT_1_SEQUENCE=TTCTGCTTTACCCTTCTTCCAA
PRIMER_RIGHT_1_SEQUENCE=GGTGTCATCATCATCAGTGGAG
PRIMER_LEFT_1=156,22
PRIMER_RIGHT_1=345,22
PRIMER_LEFT_1_TM=60.229
PRIMER_RIGHT_1_TM=60.395
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.6325
PRIMER_LEFT_2_PENALTY=0.237699
PRIMER_RIGHT_2_PENALTY=0.394838
PRIMER_LEFT_2_SEQUENCE=TCTGCTTTACCCTTCTTCCAAC
PRIMER_RIGHT_2_SEQUENCE=GGTGTCATCATCATCAGTGGAG
PRIMER_LEFT_2=157,22
PRIMER_RIGHT_2=345,22
PRIMER_LEFT_2_TM=59.762
PRIMER_RIGHT_2_TM=60.395
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.7116
PRIMER_LEFT_3_PENALTY=0.045112
PRIMER_RIGHT_3_PENALTY=0.666534
PRIMER_LEFT_3_SEQUENCE=CACCACCACTTCAAACTTCAAA
PRIMER_RIGHT_3_SEQUENCE=ATGGTGTCATCATCATCAGTGG
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=347,22
PRIMER_LEFT_3_TM=60.045
PRIMER_RIGHT_3_TM=60.667
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=271
PRIMER_PAIR_PENALTY_4=0.8566
PRIMER_LEFT_4_PENALTY=0.461740
PRIMER_RIGHT_4_PENALTY=0.394838
PRIMER_LEFT_4_SEQUENCE=CAACACCACCACTTCAAACTTC
PRIMER_RIGHT_4_SEQUENCE=GGTGTCATCATCATCAGTGGAG
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=345,22
PRIMER_LEFT_4_TM=59.538
PRIMER_RIGHT_4_TM=60.395
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_01820 Ps_contig_mira-and-tgicl-ass_franssen_16696
SEQUENCE=TCGGGTACTACGAATTTGTTTAGGACATTGAAGCTAAAGAAAACCTCATCTCTTCACCTCACCACTCGTCCCTGCAACACCACCACTTCAAACTTCAAACCCAGTTTTTTTCCTCTTTTCTTTAGCAATGGCTTATATAAAATCTTGTATTTCTTATTCTGCTTTACCCTTCTTCCAACGGAGGGGTCTACCACCTGCCTCTTCACCTTTTCATCACACAGGAACATTCATTCCCCAATGTTTAACACCAAAAATATTAAGTGCCAAGTTTTCAAAGAATGTCAGATTCTCTATTCCTAGAAGTACTACTTCTTCTTCTTCAAACTCCACTGATGATGATGACACCATTAATCAGACTAAGACTAAGAAAACACCATTTGGATATTCAAAGGAAAAGATGTTATATTAATTGGACTTGGTGTTACTTT
TARGET=331,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01821 Ps_contig_mira-and-tgicl-ass_franssen_16704
SEQUENCE=GTCVDGGHAGABTGCTTTTCYTTTTCTTTATACCATCGAAATCAAAAACACCAAAAACCAAATCCAACACAACCATAACCACCGCCACAACAACGCAACACAGTAGAAAAGAATCATCATCACACAAACACAACCAACGCATAACAACACCACCGAACCTAAACCTTTTCTCTCTTTTATTTCGTTTCCCGATTTACCCTCCCCTTCTTCTCTTATTTACCATCCTAACCCTCCTCCTCCTCCACCTTCATCTATGGCCGCGTCGCTCCTTTTCCTTTCCATAACCGCTCTCTCCCTTCTTCATCCGCATCGGCTTCTTCTTCCTCCTCCTCCGCCGTGCACTTCCCTACCACCCTTCCTTTCGTTTTCTCTATCACTCATCTCAATCGACCCAATACTACTA
TARGET=230,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01822 Ps_contig_mira-and-tgicl-ass_franssen_16704
SEQUENCE=GTCVDGGHAGABTGCTTTTCYTTTTCTTTATACCATCGAAATCAAAAACACCAAAAACCAAATCCAACACAACCATAACCACCGCCACAACAACGCAACACAGTAGAAAAGAATCATCATCACACAAACACAACCAACGCATAACAACACCACCGAACCTAAACCTTTTCTCTCTTTTATTTCGTTTCCCGATTTACCCTCCCCTTCTTCTCTTATTTACCATCCTAACCCTCCTCCTCCTCCACCTTCATCTATGGCCGCGTCGCTCCTTTTCCTTTCCATAACCGCTCTCTCCCTTCTTCATCCGCATCGGCTTCTTCTTCCTCCTCCTCCGCCGTGCACTTCCCTACCACCCTTCCTTTCGTTTTCTCTATCACTCATCTCAATCGACCCAATACTACTA
TARGET=322,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01823 Ps_contig_mira-and-tgicl-ass_franssen_16705
SEQUENCE=TCAACGCAGAGTACKCGGGACTCCCACAAACTTCCACCTTTCTTTCCTTTCTCTCTCTCTCTCTCACTCTTCCTAACAAACAAAACCACTTATAACCGGAAAAACCACTCTTCCAAAATCCATCACCACCTTCTTATATAAATATTCCCCTGTTTTCTGTGTGCTTCGTTATTCTTCTTCCGTCACAGAGTTTTTCTTTTTCTCTTTCAATCTCTTCGATGGGGAAGTACATGAAGAAACCAAAAACCTAAATCCGAATTACCTTTACTTGATTCCACCACCACTATTACCACCACAACAACCACCACAACAACCACCACCACCGCTTATTTTGGTGTTCGAACTCGTGCTAAAACCCTAGCTCTTTCTCAGAATCAGGATCTCTCTCTTTCTAATGATTCTTACCTTCAGCTTCGAAGTCGTCGCCTTCACAAACCTCCGACGCCTCAACATTCTTCTAAGAAGAATAAGCCCCAAAATCAAAACCCTAAATCTCCAATTCGTAGAGATGTTAGCAAGGAGAAAACTCCTGACGCTGAAGATGCTGACTTTGGGGAAAATGTTTTGGATTTTGAAGGTAGAGAGAGCAGAAGCACCCGAGAAACCACACCCGTCAATTTGATAAGGAACCCGGACATTCTCAGGACTCCTAGTTCGACCACCAAGCGTACTTTTTCAACTGAAGCTCATCGCAGAACGGATCATGCAACTAGAACTGTTATCCCATCTACACGGGAAATGGATGAATTCTTTGCTAAAAATGAAGCGGCTCAGCAAAAGAAGTTCATGGAGAAGTACAACTTTGATCCTGTGACAGATATGCCACTCCCGGGGCGTTACGAATGGGAAAAATTAACACCCTAGAAGACAAGCTAGTGTTTAAGTGTTGCATCAAGACATCTTTGAAGTAGCAGGGTTAGAGTTTGTTGAAATGGGTGGTGGTGTTATTTCCATTTTCCATCACTTTCTATTTACTTGTAAAGAAAGTAGGACTTTTCAACATATGTAGACTAATGATCTGTAACTTTACAGAGGTGTTGATTACAAACACAACTATACAAAGTCCTTTGTCTAACAGATCATCAAAGAGGGGAAAAAAGGGCTGTAGTTGTAGGATTTAGGTGATAAAATCAAATTAGGTCTGTATGAGGTTACAGGAATTTCCTTTTGTTCTTGTATTTTACTCTTCTTTTGTCTATACTTGTACTGATGGAACTTGAACAAACTCTTAGAAATAAAGACCAGATTCCCTCATGTAGKAATAATGTTCTGTC
TARGET=50,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01824 Ps_contig_mira-and-tgicl-ass_franssen_16705
SEQUENCE=TCAACGCAGAGTACKCGGGACTCCCACAAACTTCCACCTTTCTTTCCTTTCTCTCTCTCTCTCTCACTCTTCCTAACAAACAAAACCACTTATAACCGGAAAAACCACTCTTCCAAAATCCATCACCACCTTCTTATATAAATATTCCCCTGTTTTCTGTGTGCTTCGTTATTCTTCTTCCGTCACAGAGTTTTTCTTTTTCTCTTTCAATCTCTTCGATGGGGAAGTACATGAAGAAACCAAAAACCTAAATCCGAATTACCTTTACTTGATTCCACCACCACTATTACCACCACAACAACCACCACAACAACCACCACCACCGCTTATTTTGGTGTTCGAACTCGTGCTAAAACCCTAGCTCTTTCTCAGAATCAGGATCTCTCTCTTTCTAATGATTCTTACCTTCAGCTTCGAAGTCGTCGCCTTCACAAACCTCCGACGCCTCAACATTCTTCTAAGAAGAATAAGCCCCAAAATCAAAACCCTAAATCTCCAATTCGTAGAGATGTTAGCAAGGAGAAAACTCCTGACGCTGAAGATGCTGACTTTGGGGAAAATGTTTTGGATTTTGAAGGTAGAGAGAGCAGAAGCACCCGAGAAACCACACCCGTCAATTTGATAAGGAACCCGGACATTCTCAGGACTCCTAGTTCGACCACCAAGCGTACTTTTTCAACTGAAGCTCATCGCAGAACGGATCATGCAACTAGAACTGTTATCCCATCTACACGGGAAATGGATGAATTCTTTGCTAAAAATGAAGCGGCTCAGCAAAAGAAGTTCATGGAGAAGTACAACTTTGATCCTGTGACAGATATGCCACTCCCGGGGCGTTACGAATGGGAAAAATTAACACCCTAGAAGACAAGCTAGTGTTTAAGTGTTGCATCAAGACATCTTTGAAGTAGCAGGGTTAGAGTTTGTTGAAATGGGTGGTGGTGTTATTTCCATTTTCCATCACTTTCTATTTACTTGTAAAGAAAGTAGGACTTTTCAACATATGTAGACTAATGATCTGTAACTTTACAGAGGTGTTGATTACAAACACAACTATACAAAGTCCTTTGTCTAACAGATCATCAAAGAGGGGAAAAAAGGGCTGTAGTTGTAGGATTTAGGTGATAAAATCAAATTAGGTCTGTATGAGGTTACAGGAATTTCCTTTTGTTCTTGTATTTTACTCTTCTTTTGTCTATACTTGTACTGATGGAACTTGAACAAACTCTTAGAAATAAAGACCAGATTCCCTCATGTAGKAATAATGTTCTGTC
TARGET=313,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01825 Ps_contig_mira-and-tgicl-ass_franssen_16706
SEQUENCE=TCTTCCTAACAAACAAAACCACTTATACCGGAAAACCACTCTTCCAAATCSATCACCACCTTCTTATTATAAATATTCCCCTGTTTTCTGTGTGCTTCGTTATTCTTCTTCCGTCACAGAGTTTTTCTTTTTCTCTTTCAATCTCTTCGATGGGGGAAGTACATGAAGAAACCAAAACCTAAATCCGAATTACCTTTACTTGATTCCACCACCACTATTACCACCACAACAACCACCACCACCGCTTATTTTGGTGTTCGAACTCGTGCTAAAACCCTAGCTCTTTCTCAGAATCAGGATCTCTCTT
TARGET=232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01826 Ps_contig_mira-and-tgicl-ass_franssen_16707
SEQUENCE=GGTCKGRGBAAAGAGGKAAAAGGTTAGTTTCCCTTCTGATTSATGTGAAATAGTATTAGATTGTTTTGCATAATATTTCTATGTTATTAGATAATGGGAATGGAGTTGCTGGCTTTGAATTCATCACCACTATGCAATGTTACTACAACTACAACTACAACTAGGACTAGGAGGAGAATTCATTACAAACCCTACAATTTCAATTTCATGGCTATCATCACATCTGCTTCCAAGGTATATGATTCAGTGTTAGAGAACAAAAAGCATGTCCTTTTAACCTCTCTTCAAGACACACACAGAGGACTCTTAACAACTCCTCATCAACGTTCTTCTATTGAACAAGCACTAGTGAATGTAGAAGGGACCAACATCGGTCACCCAATTGATTTCAACAAACTCGATGGAACATGGCGCCTCCAATACACTTCTGCTCCTGATGTTCTCATTCTATTCCAAGCTGCTGCTACACTTCCTTTCTTTCAAGTTGGACAAATATTTCAGAAATTTGAATGCCGTCATAACTCTACCGGAGGTGTTATTCGAAATGTTGTCCGCTGGAGTATTCCAAATTTGTTAGAGGAACAAGAAGGTGCTACGTTGCTTGTATCTGCCAAATTTACTCTTGTATCTGCACGCAATATTTACCTTCAATTTCAAGAGATCACACTTCAAGATATAAATATTAGTGAACAGGTCCAGGCTCTAATATCTCCAGCATTACTACCGCGCTCTTTTATAAGTTTGCAGATCTTGCAATATCTACGTACTTTCAAAGCTCAAATTCCCGTGAGGGATCCAGGAAGGGAATCCGTAGGAGGATTATATTATCTTAGCTATTTGGATGATAATATGCTTTTGGGCCGTGCTGTTGGTGGAGGTGGTGTTTTTGTGTTTACAAGAGCCCAATCACTTTACTGATGACCATTCATCAGTAGTAGTTCAAAACTCGGACCGCTTGACAGAAATATTACAGTAACACTCCATGAGCCATCGTTACGGAATTGAACATATGGAGAGAAACAAATAGAACGGAGGCCTATAGCCTATTACAAAGGATTCTCCTCAATGAAATCTATGTGATATTATTTGATCTTGATATTAACTTACCGTTTTTAAGGGGGGGTAAAGGGGGGGAAGGGGGTTGGTTGGTTTGTTAAAATAATTGGTTTCTTT
TARGET=1140,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01827 Ps_contig_mira-and-tgicl-ass_franssen_16719
SEQUENCE=GGATCTCAGTTCTGACACATNAAACCCTTCGATCATGAATAANGTTGTTTGGCGGGGACGGGACGACAATTGCGTCCGCGGAGTTTGGGCGATGACAGCGCTAATGAAGGATCCGGRAGAGTGATGAAGAAAGTACAAGAAGAGATTCGAAAAGTATTTGGAGGGAAGGGTTTTATAGAGGAAGAAGATGTTCAAAAGCTTCCATATCTGAAAGCAGTTATAAAAGAAACAATGAGATTGTACCCATCTTTGCCTATTCTTTTACCTAGAGAAACCATGAAAGAGTGCGAAATCGAAGGGTACACAATTCCGAACAAGACATTAGTGTTCGTGAACGCTTGGGCGATTCATAGAGATCCAGAAGCATGGAAGAATCCAGAAGAGTTTTATCCAGAGAGATTTATAGGAAGTGATATAGATTTCAAAGGACAAGATTTCGAGCTGATTCCGTTTGGTTCGGGGCGAAGAGTTTGCCCCGGGTTAAACATGGCGGTTGCTACGGTGGATCTTTTACTTGCTAATCTTCTCTATTTGTTTGATTGGGAAATGCCTGAAGGAGTAAAGAGGGAAGATATAGATCTTGATGGTCTTCCCGGACTTGTTCAACACAAGAAACATCCTCTGTGCCTTGTTGCTAAGACTAGAATTGCATGTGTGTGATCTAGAATGTTGCATGTTATGTTATATTTATGTTAAGAAAATAAAGCATGCATGTTGTGACTAGCTTAAGCCATAACTATATATATACCATTTCCCTTGTATTATAGGTTCATATAAATAAAAATCATCTTCCTATAAGTTTATTTAGGTWACC
TARGET=738,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01828 Ps_contig_mira-and-tgicl-ass_franssen_16734
SEQUENCE=GSGATCAAAGCAAAGCAACAATTCAAAACAAATCCATCACTTCCGTTTCCGTTGGTATATCCGCCGTCGCAAAAACCATCAGAATGAGTAACAAATCCACCGGAACAGTGGTGGTTACCACCGCCACTACTACTCAACAACCACAACAACAAGTAGTTGAAACCGCCAAGAATCCATTCATCCCTTTCTTCCCCAATTTCAATTTCAACTTCAAACTCCTCCTTTTCTCCACCAAAACGCCACCATCATCATCATCAGGGTGAGGAAAAAGG
TARGET=244,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01829 Ps_contig_mira-and-tgicl-ass_franssen_16735
SEQUENCE=GATTCTATAAATAATTCAACGCAGAGTCCGCGGGGAAAGCAACAATTCAAAACAAATCCATCACTTCCGTTTCCGTTGGTATATCCGCCGTCGCAAAAACCATCAGAATGAGTAACAAATCCACCGGAACAGTGGTGGTTACCACCGCCACTACTACTCAACAACCACAACAACAAGTAGTTGAAACCGCCAAGAATCCATTCATCCCTTTCTTCCCCAATTTCAATTTCAACTTCAAACTCCCTCCTTTTTTCTTCCCACCAAAACGCCACCATCATCATCATCAGGGTGAGGAAAAAGGAAAGGCTCCGAGTGTTGCAACCTTTCCGAAAACCGAACAATCTGCCGTCGTTGTTCCCGCGCCTCTTCAGGCTCAACCTGATTCTCAGGTCCTTTCTGCCAAGACTTCCGATCCCTTTAAGCTTTACCAGATTTATGCAGTAGGAGCATTCCTTGTTTCGAGTTGGATTTGGGCAAGGTGGAATGAGAGGAAGGCTCGAGGGCGGTCTCCGAATGACGAAGATGCCAATGGTACTGGATCACGGGAGAATGAATAGATATTTGTATTACTAGTTGTATACAATGATGAATGATGCTACTTCAATATTTTCACACTGTTTCAAACAAAATTTGTTTCATTTTCATAACAGATGGAGCTGCTCGTTGTTACAGGTCAATAATAGTTAGATATTAACCCCTCAAAACTATCTTTCTATGCATTTCTAGAACCACAGGAGGTTGTAATTCTTGAATCAACATAATGAAAGTTACAATAAACTCTTTTAAATCAC
TARGET=273,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1025
PRIMER_LEFT_PENALTY=0.007205
PRIMER_RIGHT_PENALTY=0.095278
PRIMER_LEFT_SEQUENCE=GTAGTTGAAACCGCCAAGAATC
PRIMER_RIGHT_SEQUENCE=AAGCTTAAAGGGATCGGAAGTC
PRIMER_LEFT=176,22
PRIMER_RIGHT=424,22
PRIMER_LEFT_TM=60.007
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.1655
PRIMER_LEFT_1_PENALTY=0.007205
PRIMER_RIGHT_1_PENALTY=0.158297
PRIMER_LEFT_1_SEQUENCE=GTAGTTGAAACCGCCAAGAATC
PRIMER_RIGHT_1_SEQUENCE=ACAAGGAATGCTCCTACTGCAT
PRIMER_LEFT_1=176,22
PRIMER_RIGHT_1=456,22
PRIMER_LEFT_1_TM=60.007
PRIMER_RIGHT_1_TM=60.158
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.2181
PRIMER_LEFT_2_PENALTY=0.007205
PRIMER_RIGHT_2_PENALTY=0.210886
PRIMER_LEFT_2_SEQUENCE=GTAGTTGAAACCGCCAAGAATC
PRIMER_RIGHT_2_SEQUENCE=AAACAAGGAATGCTCCTACTGC
PRIMER_LEFT_2=176,22
PRIMER_RIGHT_2=458,22
PRIMER_LEFT_2_TM=60.007
PRIMER_RIGHT_2_TM=59.789
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.2238
PRIMER_LEFT_3_PENALTY=0.007205
PRIMER_RIGHT_3_PENALTY=0.216588
PRIMER_LEFT_3_SEQUENCE=GTAGTTGAAACCGCCAAGAATC
PRIMER_RIGHT_3_SEQUENCE=CGAAACAAGGAATGCTCCTACT
PRIMER_LEFT_3=176,22
PRIMER_RIGHT_3=460,22
PRIMER_LEFT_3_TM=60.007
PRIMER_RIGHT_3_TM=59.783
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.2454
PRIMER_LEFT_4_PENALTY=0.007205
PRIMER_RIGHT_4_PENALTY=0.238160
PRIMER_LEFT_4_SEQUENCE=GTAGTTGAAACCGCCAAGAATC
PRIMER_RIGHT_4_SEQUENCE=AGCTTAAAGGGATCGGAAGTCT
PRIMER_LEFT_4=176,22
PRIMER_RIGHT_4=423,22
PRIMER_LEFT_4_TM=60.007
PRIMER_RIGHT_4_TM=59.762
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_01830 Ps_contig_mira-and-tgicl-ass_franssen_16751
SEQUENCE=GTCGGGGGCTGAGAAAATGTTTGCAGAAACTGGTCTTCTCTTCCCTTACCTACAGAATTACTCACAAGAGTTTCAACAACTTGAAGAGTATTGCATGACTCACAAATCCAATGCTTCAAWGAGTGACCTTATTCAATCATCTGCAATGTCGGAATTTGATTTATTTGTAGAGGGAGATTTGTTCAAAGCACCCGAGACGATTATGGAAGAACCGGAGATGGACCTTGATCCAATGCAAGCAGCCATGTCAATGATATCTTGTGGCGAAGATATGTCGTCGCAGGGTCTAAAATCTTCGGATATTGATAATGTACTTCAAAATGAACAGCTTTTAAGTGAGGTATACTATGAGTGCAAGAAAGATCTCTTGGAAAAGGCAAATATAGAGTCACCTCTATCTGAAATTCTTGAAATCAAGGTTCCTGCTCTGAACATCGAAGAGAATTCGGTTCAAGAAAACAAACCAATTCCTGATGTGCTATTACCAAAGAGTGTCAGTTCGGGAAGTTTGAGCTCAATGGATTGGATGCATGGAGCTGCAATGAAGCCTGCTTTCCTTGATTTCCCTGGAATTGATTTCAACTCGGTTTATGGCATGCGGAGGGCTTTTAGCGAAGGAGATATAAAGACTCTTGGAAATGGTAACATGAGTGTTGGCCAATCTACTCTTGAGAGGCCTTTTCTTCACAGCAACTGCACGAGTGAGCAACGTCAAGAAAAACTCTCTAGATATAGGAATAAGAAGACAAGGAGAAACTTCGGGAGGAAAATAAAGTATGCTTGCAGGAAGGCACTGGCTGACAGTCAACCAAGAATCCGTGGAAGATTCGCGAAAACGGAGGAATCTGATGTCAAGAGGCAATGACACTTTAATTGAGCTAGTTGGAAAAAAACAAAACAAAACAAAAAAATAGAAATCAAGTTGATGCATCAGATCAAGAATCATGGTTGGAAAAGCTAGGAAGTGGTATGTGATTGGAAATTATAAACCTGATGAACTTCTGTAACTTGAGCAGGTATCTTGGAATAAGATAAACCTCAAACTCTCCTAAGTAGTTTGCCTTATATATATGCTGTAGTAGTATTCTCTTTGTAGATGGATATGACCTTCTAGATATGTACATAGGTTTGATGAAATAAAAAAAGATAAGGTTGTATCTGTAGTAACTTCTTTATTTAGTTGTATCATTAGTTAAATAATGATTGTTATGTAGTTAAGTAATTATTTAAACGT
TARGET=890,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01831 Ps_contig_mira-and-tgicl-ass_franssen_16753
SEQUENCE=GGATTTGAATGGAAAGGGCGGCACCTGTTCGCTCTCTATCCCTCACAAGCAGCACATACTTATCTCACTTCTCCGTTCTGTTCCCTTTCACTCCATCTAGGTTTTCTCCTCCTCCACCTCCTTTCCTCCGCCGCCACTCAACTTCCACCAGATTGCGTCTTACCTCCTCTTACTTCCCTAACCGCAATCGGAACCATTTCTCCACCAGAGCATCCCTTATTTCTTCTCCAGTTGCCCGAGGACTGGGGTTTGAGAAAATATCAGACGAATTCATTCCAGAATGCAAATCCAAAGCCGTGATGTTGAGGCACGTCAAGACAGGGGCTCAAGTCATGTCTGTTTCCAACCAAGATGAGAAGAAATTCAAGTGTCAACAATTCAACAAGTGTAGATCGACATTACCTGGAGTTATACTTTGTGCCAATCGGAAGCTTAGACCTGATGAATTTGTGTGGAAACGAAATCAGTCAACCCAAAATGTCGATTTCCCACCAAAGTTACCAAAGAAGAAGAAGAAACCATTCCCTATTCCCTTTAAAGAAATCAAGCAGGCTGCAAAGATGGACAAAAAACTTGCAAGCAAGGGAATAGAGAAACCTCTTGAACCTCCAAAGAATGGATTGCTTGTTCCGGACCTAGTTCCTGTTGCTTATGAAGTATTTGATGCTTGGAAGCTTTTGATTAAAGGTCTTTCTCAGCTGTTGCATGTCATTCCTGTATATGGCTGTAGTGAATGCTCGGAAGTACATGTGGCTCAGACCGGTCACTTCATTCTAGACTGTGAAGGCCGTACCAGTTCGACGCGTCATAGTTCTCATGCGTGGGTCAAGGGTTCCGTGAATGATATACTTGTCCCAATTGAGTCGTATCATCTCTTTGATCCATTTGGTAAACGCATTATGCACGATACACGGTTTGAATACGACCGGATTCCAGCTGTTGTTGAGCTCTGCATTCAAGCCGGCATGGACATCCCTGAGTATCCTTCGCGAAGAAGGACTAATCCCATACGAATGGTAGGGAGGAGAGTACTTGACCGAGGCGGTCATCTAGAGGAGCCTGAGCCGTTTCACTTTGCCGAGTCCTCTTCCATCATGGACTTTGATACTTATAGAGCCAGTGAGCGATTTCCCCATCCACCATTGTCCGACGTACCTAAAATCGCCCAAGAAACAATCAACGCATATCAAATTGTTAGAAAGGGTGTCAGGAAGTTGATGAAGAAATACACGGTGAAAGCGTGTGGCTATTGTTCGGAAGTTCATGTTGGACCGTGGGGTCATAACGCGAAGCTTTGTGGATCATTCAAACATCAATGGAGAGACGGAAAACATGGTTGGCAGGATGCTACTATAGATGAAGTCTTACCTCCAAATTATGTGTGGCATGTTAGAGACACGAATGGACCTCCAATTATGGCTGCATTGAAAAGATATTATGGAAAGGCTCCGGCCGTAGTGGAAGTGTGTGTACAAGCGGGCGCACGTATACCAGACGAGTACAGGCCGATGATGAGGCTTGACATTGTAATTCCAGATTCTGATGAGACAGGAATGATTGCTTGATGTCATCTCACAATTGCATGATCTGTTTAAGTCTAACTACACATTCATAGTCCTTAGCATGAGAAAAATCTTCAGCATGATATGTCTGATTTCTTGAATGGATACCAGCATTTGATGTATCCAACATGTATATAAGCCAATAGCAGAACAAGTAGGTTATATCACTATGTTTTGGTGTGTGAATTTTTCTATATAATATCTTAAGCTAGCGAGAAGTTATACTAAGGAAGAAMAATGTATGTTAG
TARGET=504,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01832 Ps_contig_mira-and-tgicl-ass_franssen_16754
SEQUENCE=GAGAAAACCTAGATGGAGTGAAAGGGAACAGAACGGAGAAGCGAGATAAGTATCYGCTGCTTGTGAGGGATAGAGAGCGTACAAGTGCCGCCCTTTCCATTGAAACCTACTCTCTCTGTCTCTCTCTCTTTTTCC
TARGET=119,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01833 Ps_contig_mira-and-tgicl-ass_franssen_16755
SEQUENCE=TTTGTTTGTTTGKTTGTCTTGTTTGTTCTTCTTCTACACATCAATGGCTAAGCTTAGAACTGCCATTGATTCATCATTCTGGGATTTGAACATTTCATCTCCTCAAACCCTTGACGGTTGGGCCAAACATGTTCCTGGTAACCCAATTCCTCTTGATGCTTCTGTGTCTAGTAGAGTCTTTCGCCATCAACAACTTTCAGAAATCACTTCTCATGTTCCTTTTGGTATTGTACCGTCATTGGCTCCTTCTCCTCGTAAGGAATTTGGCTCCTTTTCACTTCAGTCTCTTCTCCTTAACCTCACTGGCAATAGTTGGTGGCTTGCAACGACTGGACAATTCCGTCCAAGGAAGCTGATAGTTGATATAAAAAATGAGATTTCTAATGCTGTTCAATTCAATCTCTCCACGGTTAAGAGTGTTGCAAAGCATTTCATTGACAAGTCTCTTTATTCATACGGGTTAAACTCTCAGTTTGCCATTTCTCCTTCAACGTCGGTGCTATTTGCTTTAGAAGGCCATGGTGAGAAAGAACGTCGCCGCAAAAAAGTCACGGTTTTTCACGAGTTTTCTGATCACGATCTTACAGTGGAGGCAGCATGGCCTCAATTGTTTGTTGACCACAAAGGAAGATATTGGGATGTTCCTGAGTCTGTGTCCGTTGATTTATCTTCCCTTGTGTCTAGTTCTGGATTGCGATACCGCTTTGGGATACATAAGAACGGTGGAAATCCCCAGGCGTTTAACGCAACTGATAGCGACCCGCCATTGTCTCTACTGCCAGGGTTATGTGCCAAAGCTTCCGTTACTTACAATAAGATCAAGTACTTGTGGGAAAACACGGAGATTGGGATTGATGATGAGGACGACAAAAAAGTTTTAACTCCATATGATGTGCGACTTAAGGAACCTCATGCAGCAATATCTGGAATTATCGGTAGCTCCTGTGCTTCTTGGCTTTGGAATGGGAAGAAATTTTCCAGTACCGCTTCGGGAGAAGATCAAGCGGTGACAAAGAGAAAGAAGAGATCTCGGTTTAACGCCGATTTGTTTGGTTCAGTTTGCTTTACTTTTCAGCATGGAAGATTCATGAAGGAATACCGGGATTTAACCAGGGTAGATGCTCGTCTTGATATATCTTCAGCTTCAGGACTTGCAAAGAAGATTTTCAACAGTTTCAAGAGTTCCAGTGACGACATTAATGATCAACCGTCAGCCTCGCCCCGGCTTAACTTAATATTTCAACAGCAGGTTGCGGGACCGATTGTGTTTCGAGCAGACTCGCGGATTGCGCTTGAGTCTTTTACAAGGAAACATGGTGTGTTTGTTGAAGATTTCATTTGCAGCTTGAATTACTCGTTGAAGTCTCTCGAGTCGGGAAAGATTGTCGCTTGGTATTCACCAAAAAGGAAAGAAGGGATGGTTGAATTGCGGTTGTATGAGTTTTAACCATGGGCAATCTTCGCCATTTTTGTAACTTTCATTCATGACACTTAGAAGGCCACACATGTTTGTTGTGAAAATTGCATTTGTTGTATCAATTTAAGAGTTATTCAAAGATTCAATGGCAATTATAATATTGTTATTCTTTACTGTCATACAAACTCAAAATAAATCTAAATTTTT
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01834 Ps_contig_mira-and-tgicl-ass_franssen_16756
SEQUENCE=GAGAAAGATAACAGAATTAGGGTTAGTCGAAATTGGGGTATTTGTTTGAAGAATTAGAAAAGTGGAGGAAGACGAAGGTTTAGGAATTAGGGTTTGTGGAAATTGAAGAGAAGAAGAAAGAGAGAGAGGTGAAAAAGAAGGGAAGAGAGAAATTGAAGAGTTAGAGTGAGTGATTGCAAGGGAAAGAGTAGTGGGTTCAAAGAGAATGGAAGTTGTTGTTGTTGTGTGTGGGGAAGAGAGAGAAGATGGGGTTGGAAGGGTTAACTTGGAAGCTTCAAGAAGAACCATTAGAGAGAGAAGATGAAGAAAAAGAG
TARGET=119,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3073
PRIMER_LEFT_PENALTY=0.302565
PRIMER_RIGHT_PENALTY=0.004697
PRIMER_LEFT_SEQUENCE=GGGTTAGTCGAAATTGGGGTAT
PRIMER_RIGHT_SEQUENCE=AAGCTTCCAAGTTAACCCTTCC
PRIMER_LEFT=19,22
PRIMER_RIGHT=274,22
PRIMER_LEFT_TM=60.303
PRIMER_RIGHT_TM=60.005
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.8000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=256
PRIMER_PAIR_PENALTY_1=0.3261
PRIMER_LEFT_1_PENALTY=0.302565
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=GGGTTAGTCGAAATTGGGGTAT
PRIMER_RIGHT_1_SEQUENCE=CTTCCATTCTCTTTGAACCCAC
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=60.303
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.8000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.3261
PRIMER_LEFT_2_PENALTY=0.302565
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=GGGTTAGTCGAAATTGGGGTAT
PRIMER_RIGHT_2_SEQUENCE=ACTTCCATTCTCTTTGAACCCA
PRIMER_LEFT_2=19,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=60.303
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.8000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.3261
PRIMER_LEFT_3_PENALTY=0.302565
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=GGGTTAGTCGAAATTGGGGTAT
PRIMER_RIGHT_3_SEQUENCE=TTCCATTCTCTTTGAACCCACT
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=210,22
PRIMER_LEFT_3_TM=60.303
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.8000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=0.3368
PRIMER_LEFT_4_PENALTY=0.332137
PRIMER_RIGHT_4_PENALTY=0.004697
PRIMER_LEFT_4_SEQUENCE=GGGTTTGTGGAAATTGAAGAGA
PRIMER_RIGHT_4_SEQUENCE=AAGCTTCCAAGTTAACCCTTCC
PRIMER_LEFT_4=89,22
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=60.332
PRIMER_RIGHT_4_TM=60.005
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_01835 Ps_contig_mira-and-tgicl-ass_franssen_16756
SEQUENCE=GAGAAAGATAACAGAATTAGGGTTAGTCGAAATTGGGGTATTTGTTTGAAGAATTAGAAAAGTGGAGGAAGACGAAGGTTTAGGAATTAGGGTTTGTGGAAATTGAAGAGAAGAAGAAAGAGAGAGAGGTGAAAAAGAAGGGAAGAGAGAAATTGAAGAGTTAGAGTGAGTGATTGCAAGGGAAAGAGTAGTGGGTTCAAAGAGAATGGAAGTTGTTGTTGTTGTGTGTGGGGAAGAGAGAGAAGATGGGGTTGGAAGGGTTAACTTGGAAGCTTCAAGAAGAACCATTAGAGAGAGAAGATGAAGAAAAAGAG
TARGET=212,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3073
PRIMER_LEFT_PENALTY=0.302565
PRIMER_RIGHT_PENALTY=0.004697
PRIMER_LEFT_SEQUENCE=GGGTTAGTCGAAATTGGGGTAT
PRIMER_RIGHT_SEQUENCE=AAGCTTCCAAGTTAACCCTTCC
PRIMER_LEFT=19,22
PRIMER_RIGHT=274,22
PRIMER_LEFT_TM=60.303
PRIMER_RIGHT_TM=60.005
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.8000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=256
PRIMER_PAIR_PENALTY_1=0.3368
PRIMER_LEFT_1_PENALTY=0.332137
PRIMER_RIGHT_1_PENALTY=0.004697
PRIMER_LEFT_1_SEQUENCE=GGGTTTGTGGAAATTGAAGAGA
PRIMER_RIGHT_1_SEQUENCE=AAGCTTCCAAGTTAACCCTTCC
PRIMER_LEFT_1=89,22
PRIMER_RIGHT_1=274,22
PRIMER_LEFT_1_TM=60.332
PRIMER_RIGHT_1_TM=60.005
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=0.3518
PRIMER_LEFT_2_PENALTY=0.347129
PRIMER_RIGHT_2_PENALTY=0.004697
PRIMER_LEFT_2_SEQUENCE=ATTGCAAGGGAAAGAGTAGTGG
PRIMER_RIGHT_2_SEQUENCE=AAGCTTCCAAGTTAACCCTTCC
PRIMER_LEFT_2=172,22
PRIMER_RIGHT_2=274,22
PRIMER_LEFT_2_TM=59.653
PRIMER_RIGHT_2_TM=60.005
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.3773
PRIMER_LEFT_3_PENALTY=0.302565
PRIMER_RIGHT_3_PENALTY=0.074695
PRIMER_LEFT_3_SEQUENCE=GGGTTAGTCGAAATTGGGGTAT
PRIMER_RIGHT_3_SEQUENCE=AACCCCATCTTCTCTCTCTTCC
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=252,22
PRIMER_LEFT_3_TM=60.303
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.8000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.5052
PRIMER_LEFT_4_PENALTY=0.500551
PRIMER_RIGHT_4_PENALTY=0.004697
PRIMER_LEFT_4_SEQUENCE=AGGGTTTGTGGAAATTGAAGAG
PRIMER_RIGHT_4_SEQUENCE=AAGCTTCCAAGTTAACCCTTCC
PRIMER_LEFT_4=88,22
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=59.499
PRIMER_RIGHT_4_TM=60.005
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_01836 Ps_contig_mira-and-tgicl-ass_franssen_16764
SEQUENCE=GTTCCCAGATATGATGATGATGAAAATATAAAAGAGCAGATTCAACATGCTCTTCAACAAGTAGAATTGAAGACTAAAATCCGTGACGAAATTCGAGCTGAAAGCCAGACTAAAAAGGCCATCTGTAAAGAGTATGGGCAAGAATTTAGAGCTGCAGTACAAGAAGAGAGAGCTTCTAGGGAGTTGCTCAAATCCAAACGGAAGGAGATGGATTCAGTTCAATCCACCATGAATAGATTAAATAATGCAATTTCTGTAGGGGATATCGATAGCAAGATTAGAAACATGGAACACATGATTCAGCATGAAACTCTGCCTCTAAAAGAAGAAAAGCAATTGATACGCCAGATCAAACAATTGAAGCAGAGTCGCGGCGAGCTGTCAACTATTATAGCAAAACAGGATCAATCACAATCTCTTGACGAAAAAGACAATATTGAAGAGCAATCTAAGCAATTACAGCTCTTGAGGAAGGATTTAGATGTGCTTAGAAGTAATGTTCTGAAGGCAGAAGCAGTAACTAAAGCAGCAAAGAAGAAATATGATGAAGAAAGTAATCAAGTGAGTGAGGTTGTGGCTCAATTTAAGACTGCTGATGACATTCGTCAAGAAGCTTTTGTCAAGCTACAGACTTTGAAAAGGAAATTACATGAGAAGAGCAAATATTTTTGGGAATACAAAAATGCTTCAATTAAAGCGCAAGAACTAGCAGTACAAGGGAAGAAAGAGGAGGTGCAAAGCTTATGTATTGATGAGGCTGAGCGAATACATGAGATGTGGAAGAATGATGAGTTCAGAAGGAACTATCTCCGGTGCAACACCAGGAGTACACTGAGGAGACTGCAAACTTATGATGGCCGAATGCTTGGTCCTGATGAAGAGCCACCAGTGATTCCTAATAATACCTTCTTTGAAAAAGTTTCCAAAGATGATTCTCTGGTCTCGCGATCAGCTCCTGAACAACAAAAGAAATCAATACCAACAGAATCTGTTACTGTTAATGCAAAAGTTGAACCGGCTTCCAAGGCTGTCGTACAGAAACCTGAAATAAGTCAGATAACTAGTGCTAAAAAGCCTGCAAAACCTGCTCCTTCAGAGAAGAAGTCAGCGGTTCCAATTCGTCGTTGGGGGGACGAGTCTGATGAAGATAAGGAACCTAAGGAACCTGTGAAAACAAAGGAGGAAGAGGAGCGGATTTTAAAGGCTGAAAAGGCGAGGAAGGAGGAAGAAGAAGCAAAACTGAAGGAAATGAAAAGGCTAGAGGAAATTGAGAAAGCTAAGGAGGCACTACAAAGGAAAAAGCGTAATGCAGAGAAGGCCCAACAAAGGGCTCTCTATAAGGCACAAAAAGAAAGCTGAGCAAAAAGAGAAGGAAAGGGAGAAAAGAGCAAGGAAGAAGGGAAAGAAAAAAGACAGTTTCTACTGAAGATGTGGAGACAATAGAACAAGATTCTGCAGCTTCTCCTAGCTCTGAAACACTGACAAGAACTATAGAAGAATCTGATCAGAGTGAGAAACCAGTTGAGGTAACAAAAAGGTCAGTAAAGCCATCTCAATTCATAAAGCAGAACAAAGTGAAATCTCTACCTATGTCTATTCGCAACCGGGGAAAGAGACGAATTCAACCATGGATGTGGGTTGTAATTGCAGTTCTGGTTATTGCTGCCTTGTTTTATATTGGGAATAACAGCTCCCTGAGATCTTCGTTTCAAAACTTTGGTTTCTGAGGACACTCCAGAGTTAAAAAACTTGCTTCGATGCTAAGCAATCATTAGGTGTTTGCCTTGGGGGATGATACCCTTATATATTTATTGCATATATACATATAGAAGCAATTCTTTTGGTGCCTCTACAATTTTTTGCTTCTAGTTTTTGGTCTTTAGTCATTAATATTATTATTTTCTTCAAACCAATATTTTTTACTTTCTGATGTGATGCGATTTTAACTGGTTGATTGCTTCTGCGACCAATTATTATGGTAATAAATTATTGTGATTGTKRRWWWAGKGTTTTTTT
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01837 Ps_contig_mira-and-tgicl-ass_franssen_16782
SEQUENCE=TTTTCAAACTCTCTCTATATATATATCCATCATTGGGTTTACAAGCAACACAAACCATTCATACCTTGAAGCATTCAGAATTCAAGTTCACTTCTTTCTTCTACTACTCCTTTGAGATCATTCTATTGTCAAGAAACAAAACGAGAATGGGTTTT
TARGET=16,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01838 Ps_contig_mira-and-tgicl-ass_franssen_16787
SEQUENCE=CGTGTCACAATTTATTATGTGGTTTTTTGGGTTAGGGTTGATTGTTTTGTTGTTTGTGATACTTTTGTGYCGTTTGGTGTTTTGGATGCTCAAGAAGTTCTTTCGTTTTATCTGCTGCTGCTGAAGGATTGA
TARGET=112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01839 Ps_contig_mira-and-tgicl-ass_franssen_16810
SEQUENCE=ATACAGCCAAGAGACTTCGATCTCCACAGGAYGAAAGGGGTCAAATCGTAAAAGTAGAGGACGGTCTTCACATCATCAGTCCTGAGTTGCAAGAAGAAGAAGAACAAAGTCACAGTACAAACGGAAGGAGGAAGAGGAAGAAGAACAAGAACAAAGACACCGCAAACATAGCAAAAAAGAAGATGAGGATGAGGATGAAGAAGAAGAAGAAGAACGAGAACAAAGACACCGCAAACATAGCGAAGAAGAAGAGGAGGATGAGGATGAACCTCGCAGCTATGAGACTCGTCGAAAGTAGGAAAAACAACGCCGCAGAGAAGAAACGAGAT
TARGET=91,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01840 Ps_contig_mira-and-tgicl-ass_franssen_16810
SEQUENCE=ATACAGCCAAGAGACTTCGATCTCCACAGGAYGAAAGGGGTCAAATCGTAAAAGTAGAGGACGGTCTTCACATCATCAGTCCTGAGTTGCAAGAAGAAGAAGAACAAAGTCACAGTACAAACGGAAGGAGGAAGAGGAAGAAGAACAAGAACAAAGACACCGCAAACATAGCAAAAAAGAAGATGAGGATGAGGATGAAGAAGAAGAAGAAGAACGAGAACAAAGACACCGCAAACATAGCGAAGAAGAAGAGGAGGATGAGGATGAACCTCGCAGCTATGAGACTCGTCGAAAGTAGGAAAAACAACGCCGCAGAGAAGAAACGAGAT
TARGET=197,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01841 Ps_contig_mira-and-tgicl-ass_franssen_16811
SEQUENCE=GGACAGTGTATAGCTGTGATCAATTCTTTGGTGGTGAAGTTGGTTGTATGATGAGTGAAGGTCCCTGGCTTTTTGTTGGCATACCCAATTTTGTTAAGGCTTGGAACACACAAAATTCAATGGAACTAAGTCTAGATGGCCCTGTCGGTCAAGTTTATGCACTTGTTGTGATTAATGATATGCTCTTTGCTGGTACGCAGGATGGAGCTATATTGGTATGGAAGTTTAATGTAGCTGCCAACTGTTTCGAGCCAGCTGCATCTCTTAAAGGTCATTCCCGTGGTGTTGTTTCATTAGTTGTTGGAGCTAATAGACTTTATTCTGGTTCAATGGACAATACAATTAGAGTCTGGAACCTTGAAACCTTGCAGTGTTTACAGACACTAACAGGGCACACATCTGTTGTGATGTCTGTCCTTTGCTGGGATCAGTTCCTTATATCTTGTTCATTAGACAAAACAGTGAAGGTCTGGTTTGCTACAGAAAGTGGAAATTTGGAAGTGACATATACTCACACCGAAGAACATGGTATAGTTACTCTCTGTGGGATGCATGATTCACAAGGAAAACCCATATTGCTGTGCTCTTGCAATGATAACACTGTCCGCCTCTATGATCTGCCATCATTTGCAGAGAGAGGCAAGATATACAACAAACAAGAGGTGCGAGCAATTCAAACTGGTCCTGGTAGCATATTCTTCACCGGCGATGGAACCGGCCTAGTGAGAGTGTGGAATTGGATAGTGGAACCGGCCACAGCCACGGCCAGTAACATCCAATAAAGTGGAAGGCTCTTATAATGTATTCCAGTTAATTGTTTGAGTAGTATTTTTGATCTTCCTTTTTGTAATTTTTTAGCTGCAAGAAGGGGTGAGGTTGGGATTGCAGAGTAAAAAATAAGTGCTGGATCAAACCTGGATTCTCTGCAACCGGCCGAGGTATGGTTGAACAGACTGTAGTGCTGCAGAGTATCCGGGCTAGAACGAAAAGGAAAAGGATATATGTAATCTATGTTTGCGGGATTAGTCCTTATTTTAGAATGTGAGCTGTGGTTTTGATGTTACGTGGGTATCCTTGGCACAAAGCCTTGCTTGAGGATTGAAACATTTTGCAAAAATCACAACATTATTTAGCATTCATTGCAGATCTTTTAATGAGTATATATATAACTGAATAACTTCAAGTTACTTGTTATTTACAAGTAACCAAGATGTGTATTGTTTTTCTTTGATCGAGTGTACTTACCTATGTTTGGATCTAGTGTTTAAGCACTATTCAATTAGCATTTATTGTATGGGAGTTGGTTTATRCCAACAAGTATTTCTGCATAAGTTGATTTTCTTAAAACTGGTTTCATTAAAAAGTGTTAATTTTTTTT
TARGET=1159,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01842 Ps_contig_mira-and-tgicl-ass_franssen_16813
SEQUENCE=TATCAGGTGTTGGAGGATGAGGAAGAAGAGAATAAGGACACTGCTGCGGAATCAGAGGGAAGGGTGTTGGGAGATGGATCTGAAGCTGTGATAATTCGATACCCGTCGAATCATAGATATTATAACTGTAGTTATGATCAGGATGAAGAGGATGAGATAGTATATGAGGAATCTGATATTGATGATGAGTTTGATGAAGAGTATGATTGGGATGATGATGATGATGATGGCGGCTATGAAAGTTGCGAGAATTATGGAGATGATGAAGTATGTGATGAAAACAGTAAACAGAAGATGCTTTCTTCAAATGACAATGAGCTGGAATCGAATTCGAGTGGACGAGAGAGGAGCATGAACATGAATTCGGTTCTGCATCCGGTGGAGAATCTAAGTCAGTGGAAAGCAATCAAAGCAAAAGTTATATCAAGTAAAAACAGGAGAAAGGAAAATGTTCCATCAGAGGAAAATACAAGCATGCTGCCTTTAGTCTCACCTTGTATTTTGGAATCAAATGATCTTCAGTCGAAGCCTTTGTTGAAAGAAATCGCGGTTGATGCCAGTCTTTCAAACTGGCTGGTTTCTCCTAATTATAATGTTTCCAGTACAATTCGTTGTCAATGAAACAGAAACTTAGAGAATATAAAGTGTCAAAGTTTGATGCTTCTGTTGATTGATTTATTCATTAAGAAAATAGTCTGTCAGGATGATTTGATTGTATGTGTGACTAATTTGTGTCTTGTAATTGTTTTGTTTTTGGTTTGTGAATGGTGTTATGTTGTAATCAAAGATAGTGTGTTTGTTRAGAAAAAAACT
TARGET=211,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01843 Ps_contig_mira-and-tgicl-ass_franssen_16814
SEQUENCE=CCGATTCCAATATGGATAATTGTGAACTTGAAGAAAGGGTATGGAGGACGAACAATTGAGGCAACAATAAAACCAATGATTGAGCCTGCAATTGAACTCAAAACAACATTCATGGGAATAAACCACCAGTCAAGCATCTTCTGCAAAGTAACAGCTTGTCCTAGCTGAGAGAATATCAAACAAGGCAGCAACAATGAAAAAACCAGCCCATTCAAAAGCCTTCTTCCAGAAGCAGGCAAGATATTAACATACTTAGAAGCCATAAGAAGACCTAAAAAGCACATAGTGAAAACTTTAACTATCGGCAAAACCGCAATTTTGATTGACCCCAACACTGATTCTCCTCCAACAACCTGATTTTCCAGAGCCAGCAGTAATCTCTCCATTGTTGCCATCAAACGTCGTCGTTCCCCAATTCCAATTCCAATTCCAATTCCACAACTCTCTCTCTCTCTCTCTCACTGTTCAGTTTCTTCTGAACTGCACAGATTCATGMCTWAACTAAGNTGANCTGCAGT
TARGET=442,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01844 Ps_contig_mira-and-tgicl-ass_franssen_16815
SEQUENCE=GTCGATTGTTTCTCTCTCTCTCAATCAAATTTTTCTTGAATCAAAGACTGACCAGGCTCATCTGTAGTTTTTGCATGAAATTCTGTGCAGTTTCAGAAGAAACTGAACAGTGAGAGAGAGAGAGAGAGAGTTGNGAATGATGATGAGTGGGAACGACGTA
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01845 Ps_contig_mira-and-tgicl-ass_franssen_16815
SEQUENCE=GTCGATTGTTTCTCTCTCTCTCAATCAAATTTTTCTTGAATCAAAGACTGACCAGGCTCATCTGTAGTTTTTGCATGAAATTCTGTGCAGTTTCAGAAGAAACTGAACAGTGAGAGAGAGAGAGAGAGAGTTGNGAATGATGATGAGTGGGAACGACGTA
TARGET=112,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1642
PRIMER_LEFT_PENALTY=0.139083
PRIMER_RIGHT_PENALTY=1.025107
PRIMER_LEFT_SEQUENCE=ATCAAAGACTGACCAGGCTCAT
PRIMER_RIGHT_SEQUENCE=GTTCCCACTCATCATCATTCNC
PRIMER_LEFT=39,22
PRIMER_RIGHT=153,22
PRIMER_LEFT_TM=60.139
PRIMER_RIGHT_TM=61.025
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.3250
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.75
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=1.2845
PRIMER_LEFT_1_PENALTY=0.139083
PRIMER_RIGHT_1_PENALTY=1.145433
PRIMER_LEFT_1_SEQUENCE=ATCAAAGACTGACCAGGCTCAT
PRIMER_RIGHT_1_SEQUENCE=GTCGTTCCCACTCATCATCATT
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=156,22
PRIMER_LEFT_1_TM=60.139
PRIMER_RIGHT_1_TM=61.145
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=1.3425
PRIMER_LEFT_2_PENALTY=0.139083
PRIMER_RIGHT_2_PENALTY=1.203457
PRIMER_LEFT_2_SEQUENCE=ATCAAAGACTGACCAGGCTCAT
PRIMER_RIGHT_2_SEQUENCE=TTCCCACTCATCATCATTCNC
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=152,21
PRIMER_LEFT_2_TM=60.139
PRIMER_RIGHT_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.3250
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=1.4719
PRIMER_LEFT_3_PENALTY=0.139083
PRIMER_RIGHT_3_PENALTY=1.332802
PRIMER_LEFT_3_SEQUENCE=ATCAAAGACTGACCAGGCTCAT
PRIMER_RIGHT_3_SEQUENCE=CGTTCCCACTCATCATCATTC
PRIMER_LEFT_3=39,22
PRIMER_RIGHT_3=154,21
PRIMER_LEFT_3_TM=60.139
PRIMER_RIGHT_3_TM=60.333
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=116
PRIMER_PAIR_PENALTY_4=1.4719
PRIMER_LEFT_4_PENALTY=0.139083
PRIMER_RIGHT_4_PENALTY=1.332802
PRIMER_LEFT_4_SEQUENCE=ATCAAAGACTGACCAGGCTCAT
PRIMER_RIGHT_4_SEQUENCE=TCGTTCCCACTCATCATCATT
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=155,21
PRIMER_LEFT_4_TM=60.139
PRIMER_RIGHT_4_TM=60.333
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=117
=
PRIMER_SEQUENCE_ID=SSR_01846 Ps_contig_mira-and-tgicl-ass_franssen_16830
SEQUENCE=TACCTGTGATCAGTGATGAGAAAAGACTTCCAAAGTCCTTGTTAAGGAAAAATACGGTACCAAACTGGTGTTGGCAAGAAAGCACGATACAGCAGCAGTGTGTACTCAACCAAAGTTAACATTTGAGCCTGTCGACGGAAGTTATGGCATCTTCCATTTTTGTAGTAAAGCAGCAACAAACACTTGAAAGCCATTGTCGCTTGCCGCACCAATATGTCATTAACCAAAATGATGAATATTGCATGCCAGAAAGGTGGTGTTGCTTTTGGTGGAAACAAGGCTAGTGGAAATAAAATATCATCATGCCGATACCACCAGTAAATGCATGTCACATACAGCATGAAAGAAAAAGCAACACCAAGAAGAATAGGGACTTTCTGATCTCCCTTTAGAGCAGTCTGTTTCTTAACAATATCATTTGACTTGAACATTACAGCTGATACACTGATTGTGACAAAGAAACCTTGCAAATGCTGGCGAATCAAAACAACCACCAACAAGAGGGAAAAAGGAAGAATCTGTTCAACAAACTTCGCAATCCTCTGAAAATCAAACCTCGAATGAGTATCCCCAYCACCACCACCACCGACMACCTCAGCCAAAGCACAAGAGGAACCCTCTCCGCAGCTANAGCACGGCGAGCCCTTCCTCTGAA
TARGET=575,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01847 Ps_contig_mira-and-tgicl-ass_franssen_16853
SEQUENCE=GTCGGRGATGCACACTATTCACCCACTCACAAATTAACTTCTCTCTCTCCAAAAAATAAAGAGGAGGATTAAAAAATAGAAATAAAAAAAGAAGCTTTTTGGTAACACCACTACCCTTTATTTCATCTTCCTCACAATAGACCCAGGCGTCATCTCTCTTCACTMCCMAAAAAAACCAATAGAGATTTTCCTCAATCTCTCTCTTGATGATGAAAAAGAATAAGAAAAAACAAAAGAAAAAAGGGAAAGAAAATTTTTTACCCCTCTTCCTCTTCACACCG
TARGET=40,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01848 Ps_contig_mira-and-tgicl-ass_franssen_16854
SEQUENCE=GATGCACACTATTCACCCACTCACAAATTAACTTCTCTCTCTCCAAAAAATAAAGAGGAGGATTAAAAAATAGAAATAAAAAAAGAAAGCTTTTTGGTAACACCACTACCCTTTATTTCATCTTCCTCACAATAGACCCAGGCGTCATCTCTCTTCACTCCCAAAAAAAACCAATAGAGATTTTCCTCAATCTCTCTCTTGATGATGAAAAGAATAGAAAAACAAAAGAAAAAAAGGGAAAGAAATTTTTCCCTCTTCCTCTTCACACCGTGTCCACCCATCTCCTTCAC
TARGET=34,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6539
PRIMER_LEFT_PENALTY=0.440316
PRIMER_RIGHT_PENALTY=0.213575
PRIMER_LEFT_SEQUENCE=ATGCACACTATTCACCCACTCA
PRIMER_RIGHT_SEQUENCE=TGTGAAGAGGAAGAGGGAAAAA
PRIMER_LEFT=1,22
PRIMER_RIGHT=266,22
PRIMER_LEFT_TM=60.440
PRIMER_RIGHT_TM=60.214
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.7111
PRIMER_LEFT_1_PENALTY=0.440316
PRIMER_RIGHT_1_PENALTY=0.270774
PRIMER_LEFT_1_SEQUENCE=ATGCACACTATTCACCCACTCA
PRIMER_RIGHT_1_SEQUENCE=GTGTGAAGAGGAAGAGGGAAAA
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=267,22
PRIMER_LEFT_1_TM=60.440
PRIMER_RIGHT_1_TM=59.729
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.7310
PRIMER_LEFT_2_PENALTY=0.517385
PRIMER_RIGHT_2_PENALTY=0.213575
PRIMER_LEFT_2_SEQUENCE=GATGCACACTATTCACCCACTC
PRIMER_RIGHT_2_SEQUENCE=TGTGAAGAGGAAGAGGGAAAAA
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=266,22
PRIMER_LEFT_2_TM=59.483
PRIMER_RIGHT_2_TM=60.214
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=0.7882
PRIMER_LEFT_3_PENALTY=0.517385
PRIMER_RIGHT_3_PENALTY=0.270774
PRIMER_LEFT_3_SEQUENCE=GATGCACACTATTCACCCACTC
PRIMER_RIGHT_3_SEQUENCE=GTGTGAAGAGGAAGAGGGAAAA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=267,22
PRIMER_LEFT_3_TM=59.483
PRIMER_RIGHT_3_TM=59.729
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.9279
PRIMER_LEFT_4_PENALTY=0.440316
PRIMER_RIGHT_4_PENALTY=0.487583
PRIMER_LEFT_4_SEQUENCE=ATGCACACTATTCACCCACTCA
PRIMER_RIGHT_4_SEQUENCE=CCTGGGTCTATTGTGAGGAAGA
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=141,22
PRIMER_LEFT_4_TM=60.440
PRIMER_RIGHT_4_TM=60.488
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_01849 Ps_contig_mira-and-tgicl-ass_franssen_16873
SEQUENCE=TTCGGCGTTGAACATTCTTCCTCTGTAACAATTTCAACTTCTTCTTCTTCTTCTYTSTTGTTGTTGTTGTTTTTCTATAGGAATTGTTAAAATTGGCTTCAATGGAGCTCGTAAAGAAATTCCAGCTATAACCCATTTTCGATTATTTTCTGACTCAATTCCTCCTTCTACTTGTGTCTTTCTCTGAGTACCCCATATTGTAGTAATAAAGTGAGGAATATGAAGAAATGAAAATGTTGAGAAATGAAAAAGGGTACCCCC
TARGET=38,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01850 Ps_contig_mira-and-tgicl-ass_franssen_16873
SEQUENCE=TTCGGCGTTGAACATTCTTCCTCTGTAACAATTTCAACTTCTTCTTCTTCTTCTYTSTTGTTGTTGTTGTTTTTCTATAGGAATTGTTAAAATTGGCTTCAATGGAGCTCGTAAAGAAATTCCAGCTATAACCCATTTTCGATTATTTTCTGACTCAATTCCTCCTTCTACTTGTGTCTTTCTCTGAGTACCCCATATTGTAGTAATAAAGTGAGGAATATGAAGAAATGAAAATGTTGAGAAATGAAAAAGGGTACCCCC
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01851 Ps_contig_mira-and-tgicl-ass_franssen_16874
SEQUENCE=GGATCTTACTCTTTTCTCTTTCGATCTCTCAAAAATCTCTTCTCTTTTCCCTCTTCTCCACCCTTTTTCATTTCTCAACATTTTCATTTCTTCATATTCCTCACTTTATTACTACAATATGGGGTACTCAGAGAAAGCACAAGTAGAAGGAGGAATTGAGTCAGAAAATAATCGAAAATGGGTTATAGCTGGAATTTCTTTACGAGCTCCATTGAAGCCAATTTTAACAATTCCTATAGAAAAACAACAACAACAACAGAAAGAAGAAGAAGAAGAAGTTGAAATTGTTACAGAGGAAGAATGTTCAACACCGACAAACGAGGAATCAAAAATTCCAACGTTGTTCACGTGTCCACCTGCTCCTAGAAAGCAAAAATCTTCTTTGAAGTGGAACAGGAACTATCAAGGTAGTGGTAGTGTTGTAAGAGAGTTTTTTACAGCGCCTGATTTAGAAACCGTCTTTGTAAGACATGTTGAAACTGCGTAAAGGTTATAATAACCTTAGTTTTTAGCTAGGTGTAATAGCTCTTGTTATGTTATGTACCTTAGTTAATTTTCTGTGTTATTTTTTTTTCTAGTTATAGAGTTTCCTTTAGATTTGGACTATTTTGTATAGAGCTTTCTCAAAATGAATAAAATTATTTGTATGATTGAAGCTCTTGTTTTAATGATAATTGTGAATTTAGTTTTAGGTTAAAAACCCT
TARGET=243,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5525
PRIMER_LEFT_PENALTY=0.149516
PRIMER_RIGHT_PENALTY=0.402961
PRIMER_LEFT_SEQUENCE=CCACCCTTTTTCATTTCTCAAC
PRIMER_RIGHT_SEQUENCE=GTCGGTGTTGAACATTCTTCCT
PRIMER_LEFT=57,22
PRIMER_RIGHT=314,22
PRIMER_LEFT_TM=59.850
PRIMER_RIGHT_TM=60.403
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.6777
PRIMER_LEFT_1_PENALTY=0.149516
PRIMER_RIGHT_1_PENALTY=0.528219
PRIMER_LEFT_1_SEQUENCE=CCACCCTTTTTCATTTCTCAAC
PRIMER_RIGHT_1_SEQUENCE=CGGTGTTGAACATTCTTCCTCT
PRIMER_LEFT_1=57,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.850
PRIMER_RIGHT_1_TM=60.528
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.7596
PRIMER_LEFT_2_PENALTY=0.356592
PRIMER_RIGHT_2_PENALTY=0.402961
PRIMER_LEFT_2_SEQUENCE=ATGGGGTACTCAGAGAAAGCAC
PRIMER_RIGHT_2_SEQUENCE=GTCGGTGTTGAACATTCTTCCT
PRIMER_LEFT_2=118,22
PRIMER_RIGHT_2=314,22
PRIMER_LEFT_2_TM=59.643
PRIMER_RIGHT_2_TM=60.403
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=197
PRIMER_PAIR_PENALTY_3=0.8317
PRIMER_LEFT_3_PENALTY=0.428727
PRIMER_RIGHT_3_PENALTY=0.402961
PRIMER_LEFT_3_SEQUENCE=TCTCCACCCTTTTTCATTTCTC
PRIMER_RIGHT_3_SEQUENCE=GTCGGTGTTGAACATTCTTCCT
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=59.571
PRIMER_RIGHT_3_TM=60.403
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.8576
PRIMER_LEFT_4_PENALTY=0.454655
PRIMER_RIGHT_4_PENALTY=0.402961
PRIMER_LEFT_4_SEQUENCE=CTCCACCCTTTTTCATTTCTCA
PRIMER_RIGHT_4_SEQUENCE=GTCGGTGTTGAACATTCTTCCT
PRIMER_LEFT_4=55,22
PRIMER_RIGHT_4=314,22
PRIMER_LEFT_4_TM=60.455
PRIMER_RIGHT_4_TM=60.403
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=260
=
PRIMER_SEQUENCE_ID=SSR_01852 Ps_contig_mira-and-tgicl-ass_franssen_16874
SEQUENCE=GGATCTTACTCTTTTCTCTTTCGATCTCTCAAAAATCTCTTCTCTTTTCCCTCTTCTCCACCCTTTTTCATTTCTCAACATTTTCATTTCTTCATATTCCTCACTTTATTACTACAATATGGGGTACTCAGAGAAAGCACAAGTAGAAGGAGGAATTGAGTCAGAAAATAATCGAAAATGGGTTATAGCTGGAATTTCTTTACGAGCTCCATTGAAGCCAATTTTAACAATTCCTATAGAAAAACAACAACAACAACAGAAAGAAGAAGAAGAAGAAGTTGAAATTGTTACAGAGGAAGAATGTTCAACACCGACAAACGAGGAATCAAAAATTCCAACGTTGTTCACGTGTCCACCTGCTCCTAGAAAGCAAAAATCTTCTTTGAAGTGGAACAGGAACTATCAAGGTAGTGGTAGTGTTGTAAGAGAGTTTTTTACAGCGCCTGATTTAGAAACCGTCTTTGTAAGACATGTTGAAACTGCGTAAAGGTTATAATAACCTTAGTTTTTAGCTAGGTGTAATAGCTCTTGTTATGTTATGTACCTTAGTTAATTTTCTGTGTTATTTTTTTTTCTAGTTATAGAGTTTCCTTTAGATTTGGACTATTTTGTATAGAGCTTTCTCAAAATGAATAAAATTATTTGTATGATTGAAGCTCTTGTTTTAATGATAATTGTGAATTTAGTTTTAGGTTAAAAACCCT
TARGET=261,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5525
PRIMER_LEFT_PENALTY=0.149516
PRIMER_RIGHT_PENALTY=0.402961
PRIMER_LEFT_SEQUENCE=CCACCCTTTTTCATTTCTCAAC
PRIMER_RIGHT_SEQUENCE=GTCGGTGTTGAACATTCTTCCT
PRIMER_LEFT=57,22
PRIMER_RIGHT=314,22
PRIMER_LEFT_TM=59.850
PRIMER_RIGHT_TM=60.403
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.6777
PRIMER_LEFT_1_PENALTY=0.149516
PRIMER_RIGHT_1_PENALTY=0.528219
PRIMER_LEFT_1_SEQUENCE=CCACCCTTTTTCATTTCTCAAC
PRIMER_RIGHT_1_SEQUENCE=CGGTGTTGAACATTCTTCCTCT
PRIMER_LEFT_1=57,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.850
PRIMER_RIGHT_1_TM=60.528
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.7596
PRIMER_LEFT_2_PENALTY=0.356592
PRIMER_RIGHT_2_PENALTY=0.402961
PRIMER_LEFT_2_SEQUENCE=ATGGGGTACTCAGAGAAAGCAC
PRIMER_RIGHT_2_SEQUENCE=GTCGGTGTTGAACATTCTTCCT
PRIMER_LEFT_2=118,22
PRIMER_RIGHT_2=314,22
PRIMER_LEFT_2_TM=59.643
PRIMER_RIGHT_2_TM=60.403
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=197
PRIMER_PAIR_PENALTY_3=0.8317
PRIMER_LEFT_3_PENALTY=0.428727
PRIMER_RIGHT_3_PENALTY=0.402961
PRIMER_LEFT_3_SEQUENCE=TCTCCACCCTTTTTCATTTCTC
PRIMER_RIGHT_3_SEQUENCE=GTCGGTGTTGAACATTCTTCCT
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=59.571
PRIMER_RIGHT_3_TM=60.403
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.8576
PRIMER_LEFT_4_PENALTY=0.454655
PRIMER_RIGHT_4_PENALTY=0.402961
PRIMER_LEFT_4_SEQUENCE=CTCCACCCTTTTTCATTTCTCA
PRIMER_RIGHT_4_SEQUENCE=GTCGGTGTTGAACATTCTTCCT
PRIMER_LEFT_4=55,22
PRIMER_RIGHT_4=314,22
PRIMER_LEFT_4_TM=60.455
PRIMER_RIGHT_4_TM=60.403
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=260
=
PRIMER_SEQUENCE_ID=SSR_01853 Ps_contig_mira-and-tgicl-ass_franssen_16887
SEQUENCE=TAAACGGTTAAAGGTTAGGGGTAACGTTAAACTAAAAATTTAATTTAATTTTTCTATTTTAGTAACAAGCTTTGTATGATATTGATCTACCACTTGAGCCTTCAGTGAGCAAGACCTCATTTGCTCCATGAAACACCTAAACTCTGAGATGACATGTGGTGCCAGAGCTAATGAAACACCTCTCCAACAGACATTCTTTCCCAATAGAAGTTCAAGACGACTACTGCATACTTTTTAACCCCATTGCTGTTAAGTGCTTCTACCGGCACAGAAAACTGTGGCTCCAACTCAAGNGCCTCCTTGTAACCGCTTTTACTTAGCCTTTCCTACCTACCATTTTTCCTTCCCCTTNATAGCACTCCCCTAAGCACACCAGCCTTAGCGTCCTGGACTCAAAATCACCGACTCAGTACAAATCCGCAAAGGCGCCGAGTCAACTCGTCCGCGTTCACTCGTCGCGAGTTTCAG
TARGET=37,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9222
PRIMER_LEFT_PENALTY=0.918649
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=CGGTTAAAGGTTAGGGGTAACG
PRIMER_RIGHT_SEQUENCE=TAGGTGTTTCATGGAGCAAATG
PRIMER_LEFT=4,22
PRIMER_RIGHT=138,22
PRIMER_LEFT_TM=60.919
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=1.0433
PRIMER_LEFT_1_PENALTY=0.918649
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=CGGTTAAAGGTTAGGGGTAACG
PRIMER_RIGHT_1_SEQUENCE=TGAAGGCTCAAGTGGTAGATCA
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=103,22
PRIMER_LEFT_1_TM=60.919
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=1.1089
PRIMER_LEFT_2_PENALTY=0.918649
PRIMER_RIGHT_2_PENALTY=0.190211
PRIMER_LEFT_2_SEQUENCE=CGGTTAAAGGTTAGGGGTAACG
PRIMER_RIGHT_2_SEQUENCE=GCACCACATGTCATCTCAGAGT
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=161,22
PRIMER_LEFT_2_TM=60.919
PRIMER_RIGHT_2_TM=60.190
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.7000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=158
PRIMER_PAIR_PENALTY_3=1.1396
PRIMER_LEFT_3_PENALTY=0.918649
PRIMER_RIGHT_3_PENALTY=0.220992
PRIMER_LEFT_3_SEQUENCE=CGGTTAAAGGTTAGGGGTAACG
PRIMER_RIGHT_3_SEQUENCE=ATTGGGAAAGAATGTCTGTTGG
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=204,22
PRIMER_LEFT_3_TM=60.919
PRIMER_RIGHT_3_TM=60.221
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=1.1654
PRIMER_LEFT_4_PENALTY=0.918649
PRIMER_RIGHT_4_PENALTY=0.246779
PRIMER_LEFT_4_SEQUENCE=CGGTTAAAGGTTAGGGGTAACG
PRIMER_RIGHT_4_SEQUENCE=GCAATGGGGTTAAAAAGTATGC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=60.919
PRIMER_RIGHT_4_TM=59.753
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.7000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_01854 Ps_contig_mira-and-tgicl-ass_franssen_16889
SEQUENCE=CTTTTTKTGAAACTGATACACACTTCTCAAGTTCTCTCATACCCAGACAGATTTAGAAACAATAGTACCGAGGGTCCCTCACTGGAGACTCACAGTACCCTTGTAACCGTACCAATTTATCTGACCTCTTTTTGTGGCATAGATTGAGGTACAAACGCATATTTTCATAATCTTTTTTTCCCTGGAATGAAGGGTTTGTTTCTCTTCTCTTCTTCTTCTTCTTCTTCTTGTACTTCGACCACAGTTTCTTTCTTCACCTTCCCTTTTGTTCTCTCTTTTTATATTTTCTCCTTTTTGTTCACCAGTTTTATAGCTTGATGTGACTTGTTTTATTTTGAGTGATTGTGTGGTTAGGGTTTACATGCAAAATAAACATGTATTTTTTAGAATCTTTTTATTTTCTTATCAGAAAATAAGATAAGGTTTTGGTTTTGATTTCAGTTAATAGATCTGAGTCTGAGTCTGTAGATTCAAACAAACTTCATATATATCAGCTTCTTTTCCAAGCTAGATTCTCTCTCTCTCTATCTGTATTTTCTTTCGTTACCAAAACTAAGAAG
TARGET=208,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01855 Ps_contig_mira-and-tgicl-ass_franssen_16889
SEQUENCE=CTTTTTKTGAAACTGATACACACTTCTCAAGTTCTCTCATACCCAGACAGATTTAGAAACAATAGTACCGAGGGTCCCTCACTGGAGACTCACAGTACCCTTGTAACCGTACCAATTTATCTGACCTCTTTTTGTGGCATAGATTGAGGTACAAACGCATATTTTCATAATCTTTTTTTCCCTGGAATGAAGGGTTTGTTTCTCTTCTCTTCTTCTTCTTCTTCTTCTTGTACTTCGACCACAGTTTCTTTCTTCACCTTCCCTTTTGTTCTCTCTTTTTATATTTTCTCCTTTTTGTTCACCAGTTTTATAGCTTGATGTGACTTGTTTTATTTTGAGTGATTGTGTGGTTAGGGTTTACATGCAAAATAAACATGTATTTTTTAGAATCTTTTTATTTTCTTATCAGAAAATAAGATAAGGTTTTGGTTTTGATTTCAGTTAATAGATCTGAGTCTGAGTCTGTAGATTCAAACAAACTTCATATATATCAGCTTCTTTTCCAAGCTAGATTCTCTCTCTCTCTATCTGTATTTTCTTTCGTTACCAAAACTAAGAAG
TARGET=514,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01856 Ps_contig_mira-and-tgicl-ass_franssen_16890
SEQUENCE=TAATATATATATGTTCTTTACTAACAAGCACAGTCAGAGAAGGAGAGCATACTGCACACAAGTACAAGTCAGTAAGATTAAACACCATTGACAATAAGTTTCCTACCTCTCCTTTCTCAGAAAGGAGGAGGAGGACGAGGAGGTGGAGGACCCCAAAACATATCATGAGGCATTTGATTACCGTTATGTATAAGATTTGGTGGCAAGTTATACATAGGCATTGAAACCTGTTGTTGTTGTTGATGATGATGGTGCTGTTGTTCGGCGAAATCCAAACCTTGAGAAGAAGCAACTCCTGCTGCTACAACAGTTGCTGARTCCACACCAGATTGTTGTTCAACTTGCATTTCCTCTTGATCACCACCTTGTTCATCTTCCAATGGCAACCTCTCATAAGTAGCATTAGCAAAAGTAGCTGCAATAACCATCACAGGTCCAGAAGCAACCAAAGAACCCGCCACAGCGCCACCCACAACCTGCCCCTGACCACCGGCTAAATAAACAGTAAGCCCGGTCGCTCCAGGCGGAGATGGCGAAGGCAGAAAAGCACCCGAAAGCGACAGAATCTCAAACCTTCCATGAAGAGTAATAACACCAGCGGGAGCAGCCGGTTGCCTAAGCGTCACATTAGTCACAATGCCGCTCCCACTCAAAACCGACACACCACGGTGACGGCGGTTAGCAAAGGTAGCTATGCTCTCCGCCACATCACTACCGCTGCTAATTTCTAACACGTGGCTTCGCAGCGCGTTCGGAGATTCTTTCGTTATAACAATAGGCGGTTTGGGTTTGTTCTTCGAACCCGGCGGTCTACCTCGTGGCCTCCGACCACTACTACTACCGGGCTCCGAAGGATTCTGATCTTCAGGGTTGTTGTTATCTTCATCTTCATTATTGTTGCTGTTACTGCTATTCGTTGGAGTCGAGTTCGCGTTGTTGTTACTTTCCATTAACTCTTGCTCTCTCTTCAGCTGTTGAAGCGAAGTCGGTGATGTTGGCATCGTTGCCACATTCCCAGTCCACCATCGATTAGCCATAATCTTCTTAGTTTTTGGAAACGAAAGAAAATCAGATAGAGAGAGAGAGAATCTAGCTTAGGAAAAGAAGCTGATATATATGAAGTTTGTTGAATCTACAGACTCAGACTCAGATTA
TARGET=3,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01857 Ps_contig_mira-and-tgicl-ass_franssen_16890
SEQUENCE=TAATATATATATGTTCTTTACTAACAAGCACAGTCAGAGAAGGAGAGCATACTGCACACAAGTACAAGTCAGTAAGATTAAACACCATTGACAATAAGTTTCCTACCTCTCCTTTCTCAGAAAGGAGGAGGAGGACGAGGAGGTGGAGGACCCCAAAACATATCATGAGGCATTTGATTACCGTTATGTATAAGATTTGGTGGCAAGTTATACATAGGCATTGAAACCTGTTGTTGTTGTTGATGATGATGGTGCTGTTGTTCGGCGAAATCCAAACCTTGAGAAGAAGCAACTCCTGCTGCTACAACAGTTGCTGARTCCACACCAGATTGTTGTTCAACTTGCATTTCCTCTTGATCACCACCTTGTTCATCTTCCAATGGCAACCTCTCATAAGTAGCATTAGCAAAAGTAGCTGCAATAACCATCACAGGTCCAGAAGCAACCAAAGAACCCGCCACAGCGCCACCCACAACCTGCCCCTGACCACCGGCTAAATAAACAGTAAGCCCGGTCGCTCCAGGCGGAGATGGCGAAGGCAGAAAAGCACCCGAAAGCGACAGAATCTCAAACCTTCCATGAAGAGTAATAACACCAGCGGGAGCAGCCGGTTGCCTAAGCGTCACATTAGTCACAATGCCGCTCCCACTCAAAACCGACACACCACGGTGACGGCGGTTAGCAAAGGTAGCTATGCTCTCCGCCACATCACTACCGCTGCTAATTTCTAACACGTGGCTTCGCAGCGCGTTCGGAGATTCTTTCGTTATAACAATAGGCGGTTTGGGTTTGTTCTTCGAACCCGGCGGTCTACCTCGTGGCCTCCGACCACTACTACTACCGGGCTCCGAAGGATTCTGATCTTCAGGGTTGTTGTTATCTTCATCTTCATTATTGTTGCTGTTACTGCTATTCGTTGGAGTCGAGTTCGCGTTGTTGTTACTTTCCATTAACTCTTGCTCTCTCTTCAGCTGTTGAAGCGAAGTCGGTGATGTTGGCATCGTTGCCACATTCCCAGTCCACCATCGATTAGCCATAATCTTCTTAGTTTTTGGAAACGAAAGAAAATCAGATAGAGAGAGAGAGAATCTAGCTTAGGAAAAGAAGCTGATATATATGAAGTTTGTTGAATCTACAGACTCAGACTCAGATTA
TARGET=123,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01858 Ps_contig_mira-and-tgicl-ass_franssen_16890
SEQUENCE=TAATATATATATGTTCTTTACTAACAAGCACAGTCAGAGAAGGAGAGCATACTGCACACAAGTACAAGTCAGTAAGATTAAACACCATTGACAATAAGTTTCCTACCTCTCCTTTCTCAGAAAGGAGGAGGAGGACGAGGAGGTGGAGGACCCCAAAACATATCATGAGGCATTTGATTACCGTTATGTATAAGATTTGGTGGCAAGTTATACATAGGCATTGAAACCTGTTGTTGTTGTTGATGATGATGGTGCTGTTGTTCGGCGAAATCCAAACCTTGAGAAGAAGCAACTCCTGCTGCTACAACAGTTGCTGARTCCACACCAGATTGTTGTTCAACTTGCATTTCCTCTTGATCACCACCTTGTTCATCTTCCAATGGCAACCTCTCATAAGTAGCATTAGCAAAAGTAGCTGCAATAACCATCACAGGTCCAGAAGCAACCAAAGAACCCGCCACAGCGCCACCCACAACCTGCCCCTGACCACCGGCTAAATAAACAGTAAGCCCGGTCGCTCCAGGCGGAGATGGCGAAGGCAGAAAAGCACCCGAAAGCGACAGAATCTCAAACCTTCCATGAAGAGTAATAACACCAGCGGGAGCAGCCGGTTGCCTAAGCGTCACATTAGTCACAATGCCGCTCCCACTCAAAACCGACACACCACGGTGACGGCGGTTAGCAAAGGTAGCTATGCTCTCCGCCACATCACTACCGCTGCTAATTTCTAACACGTGGCTTCGCAGCGCGTTCGGAGATTCTTTCGTTATAACAATAGGCGGTTTGGGTTTGTTCTTCGAACCCGGCGGTCTACCTCGTGGCCTCCGACCACTACTACTACCGGGCTCCGAAGGATTCTGATCTTCAGGGTTGTTGTTATCTTCATCTTCATTATTGTTGCTGTTACTGCTATTCGTTGGAGTCGAGTTCGCGTTGTTGTTACTTTCCATTAACTCTTGCTCTCTCTTCAGCTGTTGAAGCGAAGTCGGTGATGTTGGCATCGTTGCCACATTCCCAGTCCACCATCGATTAGCCATAATCTTCTTAGTTTTTGGAAACGAAAGAAAATCAGATAGAGAGAGAGAGAATCTAGCTTAGGAAAAGAAGCTGATATATATGAAGTTTGTTGAATCTACAGACTCAGACTCAGATTA
TARGET=229,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01859 Ps_contig_mira-and-tgicl-ass_franssen_16890
SEQUENCE=TAATATATATATGTTCTTTACTAACAAGCACAGTCAGAGAAGGAGAGCATACTGCACACAAGTACAAGTCAGTAAGATTAAACACCATTGACAATAAGTTTCCTACCTCTCCTTTCTCAGAAAGGAGGAGGAGGACGAGGAGGTGGAGGACCCCAAAACATATCATGAGGCATTTGATTACCGTTATGTATAAGATTTGGTGGCAAGTTATACATAGGCATTGAAACCTGTTGTTGTTGTTGATGATGATGGTGCTGTTGTTCGGCGAAATCCAAACCTTGAGAAGAAGCAACTCCTGCTGCTACAACAGTTGCTGARTCCACACCAGATTGTTGTTCAACTTGCATTTCCTCTTGATCACCACCTTGTTCATCTTCCAATGGCAACCTCTCATAAGTAGCATTAGCAAAAGTAGCTGCAATAACCATCACAGGTCCAGAAGCAACCAAAGAACCCGCCACAGCGCCACCCACAACCTGCCCCTGACCACCGGCTAAATAAACAGTAAGCCCGGTCGCTCCAGGCGGAGATGGCGAAGGCAGAAAAGCACCCGAAAGCGACAGAATCTCAAACCTTCCATGAAGAGTAATAACACCAGCGGGAGCAGCCGGTTGCCTAAGCGTCACATTAGTCACAATGCCGCTCCCACTCAAAACCGACACACCACGGTGACGGCGGTTAGCAAAGGTAGCTATGCTCTCCGCCACATCACTACCGCTGCTAATTTCTAACACGTGGCTTCGCAGCGCGTTCGGAGATTCTTTCGTTATAACAATAGGCGGTTTGGGTTTGTTCTTCGAACCCGGCGGTCTACCTCGTGGCCTCCGACCACTACTACTACCGGGCTCCGAAGGATTCTGATCTTCAGGGTTGTTGTTATCTTCATCTTCATTATTGTTGCTGTTACTGCTATTCGTTGGAGTCGAGTTCGCGTTGTTGTTACTTTCCATTAACTCTTGCTCTCTCTTCAGCTGTTGAAGCGAAGTCGGTGATGTTGGCATCGTTGCCACATTCCCAGTCCACCATCGATTAGCCATAATCTTCTTAGTTTTTGGAAACGAAAGAAAATCAGATAGAGAGAGAGAGAATCTAGCTTAGGAAAAGAAGCTGATATATATGAAGTTTGTTGAATCTACAGACTCAGACTCAGATTA
TARGET=1075,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01860 Ps_contig_mira-and-tgicl-ass_franssen_16927
SEQUENCE=AGCCAATGGTCATGCTTCTCGGTCAATATTCCACAGGGAAAACAAGCATTGCATTGCATTTTAGTCCATTCGTTGAATCCTATCAAATTGTTACACGTTA
TARGET=46,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01861 Ps_contig_mira-and-tgicl-ass_franssen_16929
SEQUENCE=TCGGGGGATGCTAAGCTTTTTTAGTACTTACTTCTGCAACTGAAACAGAAAACCGAAAAAAAACACAAAGAACAACAAAAACAAACAAACCATGTTTTCTTCTCTACTTTGTTGTTCCATTGCAATGTGTGTTGCAATTTTCCTCCACCTATGTTCCTTTGCGGTTTCACACCCTAACAAGATCAATAATTTACCAGGTCAACCTCATGTTGAGTTTCATCAGTTTTCAGGCTATGTAAATGTGGATAAACAAAAACCAAAAGCTA
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8734
PRIMER_LEFT_PENALTY=0.598673
PRIMER_RIGHT_PENALTY=0.274768
PRIMER_LEFT_SEQUENCE=TCTGCAACTGAAACAGAAAACC
PRIMER_RIGHT_SEQUENCE=ATCTTGTTAGGGTGTGAAACCG
PRIMER_LEFT=32,22
PRIMER_RIGHT=182,22
PRIMER_LEFT_TM=59.401
PRIMER_RIGHT_TM=60.275
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=0.9432
PRIMER_LEFT_1_PENALTY=0.598673
PRIMER_RIGHT_1_PENALTY=0.344527
PRIMER_LEFT_1_SEQUENCE=TCTGCAACTGAAACAGAAAACC
PRIMER_RIGHT_1_SEQUENCE=CAAAGGAACATAGGTGGAGGAA
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=160,22
PRIMER_LEFT_1_TM=59.401
PRIMER_RIGHT_1_TM=60.345
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=1.2223
PRIMER_LEFT_2_PENALTY=0.598673
PRIMER_RIGHT_2_PENALTY=0.623595
PRIMER_LEFT_2_SEQUENCE=TCTGCAACTGAAACAGAAAACC
PRIMER_RIGHT_2_SEQUENCE=AACATAGGTGGAGGAAAATTGC
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=59.401
PRIMER_RIGHT_2_TM=59.376
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=1.2395
PRIMER_LEFT_3_PENALTY=0.598673
PRIMER_RIGHT_3_PENALTY=0.640802
PRIMER_LEFT_3_SEQUENCE=TCTGCAACTGAAACAGAAAACC
PRIMER_RIGHT_3_SEQUENCE=GCCTGAAAACTGATGAAACTCA
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=231,22
PRIMER_LEFT_3_TM=59.401
PRIMER_RIGHT_3_TM=59.359
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=1.4757
PRIMER_LEFT_4_PENALTY=0.598673
PRIMER_RIGHT_4_PENALTY=0.876993
PRIMER_LEFT_4_SEQUENCE=TCTGCAACTGAAACAGAAAACC
PRIMER_RIGHT_4_SEQUENCE=GTGAAACCGCAAAGGAACATAG
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=169,22
PRIMER_LEFT_4_TM=59.401
PRIMER_RIGHT_4_TM=60.877
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_01862 Ps_contig_mira-and-tgicl-ass_franssen_16958
SEQUENCE=CTCCTTCTCTTTTGCCTTCTCTTCCTCCCTCTTTTTTCTTTTGCATCGACTTTGGGGTCGGTTTTGGGCTGCACGGAAGACTTAAGATTCTGAACATATTTCTGAACTGCTTTGCTTTTGTTTTTGTTCTTGAGACCAAAAGTTTTGTCTTCAACAACCTTCTGCTTCTTCGCTACATCAGCTTTTGATTGCTTCGGAGGCATCTCGTATCGGCATCAAAATTTCAAATAAATATATAAATTATATGAAGTAGAATCGATAATCAAGTTACGAGAAGGAGGAGGAGAAGAAGAAGAAGTGAGGGATTGTTGAAGGGGAAGATTGGTGGGTCTGTACCTGCTGCCAATGTTAGGGTTAGATTAGGGTTTCGAGATTCTGGATTCGAGAGAAGGTTACGAAAACTGGAATTTCCGATCTGCCCCTGAATCCAGTSYSCGWCC
TARGET=285,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01863 Ps_contig_mira-and-tgicl-ass_franssen_16959
SEQUENCE=CCTAAAGTGTTTCTAAAACAATGATTGCTCTTTCCTTCATGATCGTTCTTCTTCTTACTCATTTGGCCATTTTTAGTTACTATTGCTGTTGCTACTACTACTACTGTTGCGACTTACACAGTTTCTATGATTACTGCTACGAACCTTTAGGTCTTCCAGTCCTATTTCTGGTGTAGGGACCACAACCTAAACGGAAATTTCCCAAAGTGATGACTTTCTACTTTGAGCTTATTGTACTTTCGTGGACATG
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3515
PRIMER_LEFT_PENALTY=1.117398
PRIMER_RIGHT_PENALTY=0.234069
PRIMER_LEFT_SEQUENCE=TGCTCTTTCCTTCATGATCGTT
PRIMER_RIGHT_SEQUENCE=TGGTCCCTACACCAGAAATAGG
PRIMER_LEFT=25,22
PRIMER_RIGHT=181,22
PRIMER_LEFT_TM=61.117
PRIMER_RIGHT_TM=60.234
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=1.3652
PRIMER_LEFT_1_PENALTY=1.117398
PRIMER_RIGHT_1_PENALTY=0.247794
PRIMER_LEFT_1_SEQUENCE=TGCTCTTTCCTTCATGATCGTT
PRIMER_RIGHT_1_SEQUENCE=GAAATTTCCGTTTAGGTTGTGG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=200,22
PRIMER_LEFT_1_TM=61.117
PRIMER_RIGHT_1_TM=59.752
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=1.7582
PRIMER_LEFT_2_PENALTY=1.117398
PRIMER_RIGHT_2_PENALTY=0.640844
PRIMER_LEFT_2_SEQUENCE=TGCTCTTTCCTTCATGATCGTT
PRIMER_RIGHT_2_SEQUENCE=TTGTGGTCCCTACACCAGAAAT
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=184,22
PRIMER_LEFT_2_TM=61.117
PRIMER_RIGHT_2_TM=60.641
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=1.7626
PRIMER_LEFT_3_PENALTY=1.117398
PRIMER_RIGHT_3_PENALTY=0.645218
PRIMER_LEFT_3_SEQUENCE=TGCTCTTTCCTTCATGATCGTT
PRIMER_RIGHT_3_SEQUENCE=TGTGGTCCCTACACCAGAAATA
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=183,22
PRIMER_LEFT_3_TM=61.117
PRIMER_RIGHT_3_TM=59.355
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=1.7691
PRIMER_LEFT_4_PENALTY=1.117398
PRIMER_RIGHT_4_PENALTY=0.651681
PRIMER_LEFT_4_SEQUENCE=TGCTCTTTCCTTCATGATCGTT
PRIMER_RIGHT_4_SEQUENCE=GGTCCCTACACCAGAAATAGGA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=180,22
PRIMER_LEFT_4_TM=61.117
PRIMER_RIGHT_4_TM=59.348
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=156
=
PRIMER_SEQUENCE_ID=SSR_01864 Ps_contig_mira-and-tgicl-ass_franssen_16960
SEQUENCE=TAGGTCTTCCAGTCCTATTTCTGGTGTAGGGACCACACCTAAACGGAAAATTTCCCAAAGTGATGACTTTCTACTTTGAGCTATTGACTTTCGTGGACATGATGATTTTAACTTGTGGCTTTGGACTTGGATGTGTGTGAAACTGGTTTCTAACTTTGGTTTTTGTTAATTGATACTATTGGAGTTGTCGTTGTTGTTGTTGAACTAGTGCTACTTGATGTTGGTTGAGTTTTGACTTGTACTACTATTGCTACGAAACTCATTGAAACAACCATTGTTAAAGCTCAAAGGTTCTTGCTTTGATTTGAACTGCTTACCCTGGT
TARGET=190,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1714
PRIMER_LEFT_PENALTY=0.069737
PRIMER_RIGHT_PENALTY=0.101680
PRIMER_LEFT_SEQUENCE=ACTTGGATGTGTGTGAAACTGG
PRIMER_RIGHT_SEQUENCE=ATCAAAGCAAGAACCTTTGAGC
PRIMER_LEFT=124,22
PRIMER_RIGHT=303,22
PRIMER_LEFT_TM=59.930
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=0.1714
PRIMER_LEFT_1_PENALTY=0.069737
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=CTTGGATGTGTGTGAAACTGGT
PRIMER_RIGHT_1_SEQUENCE=ATCAAAGCAAGAACCTTTGAGC
PRIMER_LEFT_1=125,22
PRIMER_RIGHT_1=303,22
PRIMER_LEFT_1_TM=59.930
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=179
PRIMER_PAIR_PENALTY_2=0.2190
PRIMER_LEFT_2_PENALTY=0.117281
PRIMER_RIGHT_2_PENALTY=0.101680
PRIMER_LEFT_2_SEQUENCE=TTGAGCTATTGACTTTCGTGGA
PRIMER_RIGHT_2_SEQUENCE=ATCAAAGCAAGAACCTTTGAGC
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=303,22
PRIMER_LEFT_2_TM=59.883
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=0.3357
PRIMER_LEFT_3_PENALTY=0.234069
PRIMER_RIGHT_3_PENALTY=0.101680
PRIMER_LEFT_3_SEQUENCE=CCTATTTCTGGTGTAGGGACCA
PRIMER_RIGHT_3_SEQUENCE=ATCAAAGCAAGAACCTTTGAGC
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=303,22
PRIMER_LEFT_3_TM=60.234
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.5436
PRIMER_LEFT_4_PENALTY=0.069737
PRIMER_RIGHT_4_PENALTY=0.473868
PRIMER_LEFT_4_SEQUENCE=ACTTGGATGTGTGTGAAACTGG
PRIMER_RIGHT_4_SEQUENCE=ACCAGGGTAAGCAGTTCAAATC
PRIMER_LEFT_4=124,22
PRIMER_RIGHT_4=322,22
PRIMER_LEFT_4_TM=59.930
PRIMER_RIGHT_4_TM=59.526
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_01865 Ps_contig_mira-and-tgicl-ass_franssen_16968
SEQUENCE=GAACACTCACCACTTTCCTCTCTCAACCTCTTCTCTTCTCATCTCATCATCTTCTCCCATGGCTTCTTCCACCACAGACCTCAAACAACGATTCGTCACACCCGACCAAACCACCACCACCAACACAACCACAACAAACGACGACGACAAAAAATGGAACAAGCAACCACAAAGAGACAAACGAATGGCAATGGCCACACGCGGTCTTAGATCACTCATAATAGCGGTTTTCG
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4024
PRIMER_LEFT_PENALTY=0.221594
PRIMER_RIGHT_PENALTY=0.180765
PRIMER_LEFT_SEQUENCE=CACCACTTTCCTCTCTCAACCT
PRIMER_RIGHT_SEQUENCE=TGTCTCTTTGTGGTTGCTTGTT
PRIMER_LEFT=7,22
PRIMER_RIGHT=178,22
PRIMER_LEFT_TM=59.778
PRIMER_RIGHT_TM=59.819
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.4024
PRIMER_LEFT_1_PENALTY=0.221594
PRIMER_RIGHT_1_PENALTY=0.180765
PRIMER_LEFT_1_SEQUENCE=CACCACTTTCCTCTCTCAACCT
PRIMER_RIGHT_1_SEQUENCE=TTGTCTCTTTGTGGTTGCTTGT
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=179,22
PRIMER_LEFT_1_TM=59.778
PRIMER_RIGHT_1_TM=59.819
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.5394
PRIMER_LEFT_2_PENALTY=0.221594
PRIMER_RIGHT_2_PENALTY=0.317850
PRIMER_LEFT_2_SEQUENCE=CACCACTTTCCTCTCTCAACCT
PRIMER_RIGHT_2_SEQUENCE=TTTGTCTCTTTGTGGTTGCTTG
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=180,22
PRIMER_LEFT_2_TM=59.778
PRIMER_RIGHT_2_TM=60.318
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.5420
PRIMER_LEFT_3_PENALTY=0.361206
PRIMER_RIGHT_3_PENALTY=0.180765
PRIMER_LEFT_3_SEQUENCE=CACAGACCTCAAACAACGATTC
PRIMER_RIGHT_3_SEQUENCE=TGTCTCTTTGTGGTTGCTTGTT
PRIMER_LEFT_3=72,22
PRIMER_RIGHT_3=178,22
PRIMER_LEFT_3_TM=59.639
PRIMER_RIGHT_3_TM=59.819
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=0.6791
PRIMER_LEFT_4_PENALTY=0.361206
PRIMER_RIGHT_4_PENALTY=0.317850
PRIMER_LEFT_4_SEQUENCE=CACAGACCTCAAACAACGATTC
PRIMER_RIGHT_4_SEQUENCE=TTTGTCTCTTTGTGGTTGCTTG
PRIMER_LEFT_4=72,22
PRIMER_RIGHT_4=180,22
PRIMER_LEFT_4_TM=59.639
PRIMER_RIGHT_4_TM=60.318
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_01866 Ps_contig_mira-and-tgicl-ass_franssen_16984
SEQUENCE=AAACCTTTACGTGTGTTTTACTTGATTCACATCATGCAGATCTTGAAAAACCAGAAACAGAAGAAACCCATCATGGAGAAGATGAAGAGAAACAAGAAAATGAAGAAAGTTCGGAACTTCCTTCTTCTTCTTCTTCTTCTTCACCTGAAATATCAGTAGCTTCTTCTAGTACAAGAAAACGATGAAGCAGAAGTAGAATCAGAAAAAGTTTCAGCGGAGGAAGAAGAAGAGAAGGAACAACCACCGACCCATCAAGGATCCGACCCGGATATTGTTCCTATTGGGTCAATGGAGCGAGAAAATGGGTCAAAGAAAGAAGAGAGAGAAAGAGAAACGTTACCGGAATGCTTGTTATTGATGATGTGCGAGCCAAAACTTTCCATGGAAGTGTCTAAAGAAACATGGGTATGCAGCACCGATTTCATACGGT
TARGET=121,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1143
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.102139
PRIMER_LEFT_SEQUENCE=AACCAGAAACAGAAGAAACCCA
PRIMER_RIGHT_SEQUENCE=CTTTCTTTGACCCATTTTCTCG
PRIMER_LEFT=48,22
PRIMER_RIGHT=315,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=0.1199
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.107763
PRIMER_LEFT_1_SEQUENCE=AACCAGAAACAGAAGAAACCCA
PRIMER_RIGHT_1_SEQUENCE=CTCCGCTGAAACTTTTTCTGAT
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=218,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=171
PRIMER_PAIR_PENALTY_2=0.1257
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.102139
PRIMER_LEFT_2_SEQUENCE=CCAGAAACAGAAGAAACCCATC
PRIMER_RIGHT_2_SEQUENCE=CTTTCTTTGACCCATTTTCTCG
PRIMER_LEFT_2=50,22
PRIMER_RIGHT_2=315,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.1313
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.107763
PRIMER_LEFT_3_SEQUENCE=CCAGAAACAGAAGAAACCCATC
PRIMER_RIGHT_3_SEQUENCE=CTCCGCTGAAACTTTTTCTGAT
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=218,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=0.1620
PRIMER_LEFT_4_PENALTY=0.059875
PRIMER_RIGHT_4_PENALTY=0.102139
PRIMER_LEFT_4_SEQUENCE=GAAGAAACCCATCATGGAGAAG
PRIMER_RIGHT_4_SEQUENCE=CTTTCTTTGACCCATTTTCTCG
PRIMER_LEFT_4=59,22
PRIMER_RIGHT_4=315,22
PRIMER_LEFT_4_TM=59.940
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_01867 Ps_contig_mira-and-tgicl-ass_franssen_16994
SEQUENCE=CCTTACTTGTGGGAAACATTGGGATTTTTTGGTTGGTTGGATTGATTTGGGCACTTGTTGTTTTGGTTAGAAAAGATATGAAGCTTAATGCAATGTTGGGTGGAGGGCTTTTGGCTATTTACTTTAGTTCTGTTATTCTTAGGCTTATACAAACACTTGGGCCTCTTCAATTTAAGGATATGTTATAGTGTGAGTCTTAGAAAAGATAACGAGAATTATATATTAGAAGCTGACATTAGTTTGTGTACATGGATTATGTTTATGTAAGTATATGGTAGGGTTGGAGTTTATTTCTTAGGGCCTAGTAGGGGTATACAAGTTTAACTTCCACTT
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4687
PRIMER_LEFT_PENALTY=0.462913
PRIMER_RIGHT_PENALTY=0.005761
PRIMER_LEFT_SEQUENCE=TACTTGTGGGAAACATTGGGAT
PRIMER_RIGHT_SEQUENCE=AGTAAATAGCCAAAAGCCCTCC
PRIMER_LEFT=3,22
PRIMER_RIGHT=122,22
PRIMER_LEFT_TM=60.463
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.3000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=0.6198
PRIMER_LEFT_1_PENALTY=0.614038
PRIMER_RIGHT_1_PENALTY=0.005761
PRIMER_LEFT_1_SEQUENCE=CTTACTTGTGGGAAACATTGGG
PRIMER_RIGHT_1_SEQUENCE=AGTAAATAGCCAAAAGCCCTCC
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=122,22
PRIMER_LEFT_1_TM=60.614
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=0.6198
PRIMER_LEFT_2_PENALTY=0.614038
PRIMER_RIGHT_2_PENALTY=0.005761
PRIMER_LEFT_2_SEQUENCE=CCTTACTTGTGGGAAACATTGG
PRIMER_RIGHT_2_SEQUENCE=AGTAAATAGCCAAAAGCCCTCC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=122,22
PRIMER_LEFT_2_TM=60.614
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.9251
PRIMER_LEFT_3_PENALTY=0.462913
PRIMER_RIGHT_3_PENALTY=0.462203
PRIMER_LEFT_3_SEQUENCE=TACTTGTGGGAAACATTGGGAT
PRIMER_RIGHT_3_SEQUENCE=TTAAATTGAAGAGGCCCAAGTG
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=60.463
PRIMER_RIGHT_3_TM=60.462
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.9368
PRIMER_LEFT_4_PENALTY=0.462913
PRIMER_RIGHT_4_PENALTY=0.473868
PRIMER_LEFT_4_SEQUENCE=TACTTGTGGGAAACATTGGGAT
PRIMER_RIGHT_4_SEQUENCE=GAAGAGGCCCAAGTGTTTGTAT
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=167,22
PRIMER_LEFT_4_TM=60.463
PRIMER_RIGHT_4_TM=59.526
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.3000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_01868 Ps_contig_mira-and-tgicl-ass_franssen_17006
SEQUENCE=CAGCGATCTGGTTCCACAGCAGAACCCTTGGACCAGCGATCTGGATTCATGCCAGAATCACGATAATCAGAAGACCTAACAGGAAGAGCTTTTTTACCAACCGCCCAATTGTCCACTCCATCAGCCTTAGATTCACCACCAAAAGCACCTCCACCACCACCACCACCACCGCCGCCACTGTATCGATTTTGCCGGCCTGAATCAAACGAGGGAAGTGGCTTTTTCACCGAAGCCCAATTATCCACCTCGTCAGCCCTCGAATGTTGATCCAAATCCCGAAACCCTAGAATTCGGTGCCCTGCGTTCATCATCAAATCCACCATAGGATCTTCTTCCAGCACCCCAC
TARGET=151,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0963
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.095574
PRIMER_LEFT_SEQUENCE=AGCCTTAGATTCACCACCAAAA
PRIMER_RIGHT_SEQUENCE=GGTGCTGGAAGAAGATCCTATG
PRIMER_LEFT=122,22
PRIMER_RIGHT=341,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.0963
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=AGCCTTAGATTCACCACCAAAA
PRIMER_RIGHT_1_SEQUENCE=GTGCTGGAAGAAGATCCTATGG
PRIMER_LEFT_1=122,22
PRIMER_RIGHT_1=340,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=0.0963
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=GCCTTAGATTCACCACCAAAAG
PRIMER_RIGHT_2_SEQUENCE=GGTGCTGGAAGAAGATCCTATG
PRIMER_LEFT_2=123,22
PRIMER_RIGHT_2=341,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.0963
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=GCCTTAGATTCACCACCAAAAG
PRIMER_RIGHT_3_SEQUENCE=GTGCTGGAAGAAGATCCTATGG
PRIMER_LEFT_3=123,22
PRIMER_RIGHT_3=340,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.0963
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.095574
PRIMER_LEFT_4_SEQUENCE=AGCCTTAGATTCACCACCAAAA
PRIMER_RIGHT_4_SEQUENCE=TGCTGGAAGAAGATCCTATGGT
PRIMER_LEFT_4=122,22
PRIMER_RIGHT_4=339,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_01869 Ps_contig_mira-and-tgicl-ass_franssen_17007
SEQUENCE=TTTCGAATAAACTCTAATGCCTGTTGAAAACCGGACAACAGCTTGCTTCCAGCAGTTCCCAACTCATCAATCTTTGTAAACCTTGTCATGAAATCTCTGAATTTGTTTAAAATTTCTTTCTCCAAGTCAAAAATTGATTTGTCTTCCATGGTTTCCATTCTGTGGAATCCCAGTAGAAGGAAGAGAGATGGTGAAGAAGAAATTGAAAATCACAGCGAACCAGTAGCGTGCTTCAATGCTGGAATCTGATGATGATGATGATGGTGATGTTCTAGGAAATTCCTATGAAAGACCAGAAAGACTAGTTAGGGAGTGTGAACCGCCGGTTTGTACAATAAGCGGCCGTCGACAGATTTTTGTTTTTTCCACTTAATTCTATTTAAACTAAGATGCT
TARGET=247,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8153
PRIMER_LEFT_PENALTY=0.067516
PRIMER_RIGHT_PENALTY=1.747794
PRIMER_LEFT_SEQUENCE=TGGAATCCCAGTAGAAGGAAGA
PRIMER_RIGHT_SEQUENCE=GCGGTTCACACTCCCTAACTA
PRIMER_LEFT=162,22
PRIMER_RIGHT=322,21
PRIMER_LEFT_TM=60.068
PRIMER_RIGHT_TM=59.252
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=2.2354
PRIMER_LEFT_1_PENALTY=0.487583
PRIMER_RIGHT_1_PENALTY=1.747794
PRIMER_LEFT_1_SEQUENCE=TCTGTGGAATCCCAGTAGAAGG
PRIMER_RIGHT_1_SEQUENCE=GCGGTTCACACTCCCTAACTA
PRIMER_LEFT_1=158,22
PRIMER_RIGHT_1=322,21
PRIMER_LEFT_1_TM=60.488
PRIMER_RIGHT_1_TM=59.252
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=2.2354
PRIMER_LEFT_2_PENALTY=0.487583
PRIMER_RIGHT_2_PENALTY=1.747794
PRIMER_LEFT_2_SEQUENCE=CTGTGGAATCCCAGTAGAAGGA
PRIMER_RIGHT_2_SEQUENCE=GCGGTTCACACTCCCTAACTA
PRIMER_LEFT_2=159,22
PRIMER_RIGHT_2=322,21
PRIMER_LEFT_2_TM=60.488
PRIMER_RIGHT_2_TM=59.252
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=2.2527
PRIMER_LEFT_3_PENALTY=0.504940
PRIMER_RIGHT_3_PENALTY=1.747794
PRIMER_LEFT_3_SEQUENCE=AGCAGTTCCCAACTCATCAATC
PRIMER_RIGHT_3_SEQUENCE=GCGGTTCACACTCCCTAACTA
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=322,21
PRIMER_LEFT_3_TM=60.505
PRIMER_RIGHT_3_TM=59.252
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=2.2527
PRIMER_LEFT_4_PENALTY=0.504940
PRIMER_RIGHT_4_PENALTY=1.747794
PRIMER_LEFT_4_SEQUENCE=GCAGTTCCCAACTCATCAATCT
PRIMER_RIGHT_4_SEQUENCE=GCGGTTCACACTCCCTAACTA
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=322,21
PRIMER_LEFT_4_TM=60.505
PRIMER_RIGHT_4_TM=59.252
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_01870 Ps_contig_mira-and-tgicl-ass_franssen_17030
SEQUENCE=ATGGGAGAATACAGGACCTAGAATCCTTAGGTCGTCTGGCAGGAATTGCGGCATGGCGGATGATCGTATTTTTCTCCCACTCCACGAGCTCCGACTTGCCTGAACGGTCGAACCGCATCCATAACCTAGCTCCTACCGGTTTTTTTCTTTACTTTTGTAACCAATCGGTTATCCGAAGGTGAACCGATGATTTCAACCGCCGTAGGATGCGGCGGAGAATGAGTCGGCACGGCGGATTTCTTAGACCGCCGGCGAAAGGAGAAGAGGCCCTTGCTCTTCTTACCCATATTTAACCGGAGATTAATATATAATACAATAAATTAATAGCTAATAATAATAATATAATAATGGTTGAACATTGAAAATGCGATGAGAAAGAAAGAAAGGAAGAAGAGAGTGATGGTGATGTTGGTGTTTTTCTGTTTCTCCCTCTTCGCTTTGATTTTCTTCCATAAACGGCTTTGACTAAGGCAATTTTAATTCTGAGAGATAGTATAGAACTATAGATGAAGAATAAACGAATCCCCGCGTA
TARGET=329,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3914
PRIMER_LEFT_PENALTY=0.379254
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=AAGAGGCCCTTGCTCTTCTTAC
PRIMER_RIGHT_SEQUENCE=AGGGAGAAACAGAAAAACACCA
PRIMER_LEFT=261,22
PRIMER_RIGHT=430,22
PRIMER_LEFT_TM=60.379
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.3964
PRIMER_LEFT_1_PENALTY=0.379254
PRIMER_RIGHT_1_PENALTY=0.017127
PRIMER_LEFT_1_SEQUENCE=AAGAGGCCCTTGCTCTTCTTAC
PRIMER_RIGHT_1_SEQUENCE=CCTTAGTCAAAGCCGTTTATGG
PRIMER_LEFT_1=261,22
PRIMER_RIGHT_1=470,22
PRIMER_LEFT_1_TM=60.379
PRIMER_RIGHT_1_TM=60.017
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.4134
PRIMER_LEFT_2_PENALTY=0.379254
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=AAGAGGCCCTTGCTCTTCTTAC
PRIMER_RIGHT_2_SEQUENCE=TCCTTTCTTTCTTTCTCATCGC
PRIMER_LEFT_2=261,22
PRIMER_RIGHT_2=387,22
PRIMER_LEFT_2_TM=60.379
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=9.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=127
PRIMER_PAIR_PENALTY_3=0.4510
PRIMER_LEFT_3_PENALTY=0.416895
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=AACCTAGCTCCTACCGGTTTTT
PRIMER_RIGHT_3_SEQUENCE=TCCTTTCTTTCTTTCTCATCGC
PRIMER_LEFT_3=122,22
PRIMER_RIGHT_3=387,22
PRIMER_LEFT_3_TM=59.583
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=9.8000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=0.5138
PRIMER_LEFT_4_PENALTY=0.379254
PRIMER_RIGHT_4_PENALTY=0.134571
PRIMER_LEFT_4_SEQUENCE=AAGAGGCCCTTGCTCTTCTTAC
PRIMER_RIGHT_4_SEQUENCE=CGAAGAGGGAGAAACAGAAAAA
PRIMER_LEFT_4=261,22
PRIMER_RIGHT_4=435,22
PRIMER_LEFT_4_TM=60.379
PRIMER_RIGHT_4_TM=59.865
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_01871 Ps_contig_mira-and-tgicl-ass_franssen_17030
SEQUENCE=ATGGGAGAATACAGGACCTAGAATCCTTAGGTCGTCTGGCAGGAATTGCGGCATGGCGGATGATCGTATTTTTCTCCCACTCCACGAGCTCCGACTTGCCTGAACGGTCGAACCGCATCCATAACCTAGCTCCTACCGGTTTTTTTCTTTACTTTTGTAACCAATCGGTTATCCGAAGGTGAACCGATGATTTCAACCGCCGTAGGATGCGGCGGAGAATGAGTCGGCACGGCGGATTTCTTAGACCGCCGGCGAAAGGAGAAGAGGCCCTTGCTCTTCTTACCCATATTTAACCGGAGATTAATATATAATACAATAAATTAATAGCTAATAATAATAATATAATAATGGTTGAACATTGAAAATGCGATGAGAAAGAAAGAAAGGAAGAAGAGAGTGATGGTGATGTTGGTGTTTTTCTGTTTCTCCCTCTTCGCTTTGATTTTCTTCCATAAACGGCTTTGACTAAGGCAATTTTAATTCTGAGAGATAGTATAGAACTATAGATGAAGAATAAACGAATCCCCGCGTA
TARGET=373,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3914
PRIMER_LEFT_PENALTY=0.379254
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=AAGAGGCCCTTGCTCTTCTTAC
PRIMER_RIGHT_SEQUENCE=AGGGAGAAACAGAAAAACACCA
PRIMER_LEFT=261,22
PRIMER_RIGHT=430,22
PRIMER_LEFT_TM=60.379
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.3964
PRIMER_LEFT_1_PENALTY=0.379254
PRIMER_RIGHT_1_PENALTY=0.017127
PRIMER_LEFT_1_SEQUENCE=AAGAGGCCCTTGCTCTTCTTAC
PRIMER_RIGHT_1_SEQUENCE=CCTTAGTCAAAGCCGTTTATGG
PRIMER_LEFT_1=261,22
PRIMER_RIGHT_1=470,22
PRIMER_LEFT_1_TM=60.379
PRIMER_RIGHT_1_TM=60.017
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.5138
PRIMER_LEFT_2_PENALTY=0.379254
PRIMER_RIGHT_2_PENALTY=0.134571
PRIMER_LEFT_2_SEQUENCE=AAGAGGCCCTTGCTCTTCTTAC
PRIMER_RIGHT_2_SEQUENCE=CGAAGAGGGAGAAACAGAAAAA
PRIMER_LEFT_2=261,22
PRIMER_RIGHT_2=435,22
PRIMER_LEFT_2_TM=60.379
PRIMER_RIGHT_2_TM=59.865
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=175
PRIMER_PAIR_PENALTY_3=0.5266
PRIMER_LEFT_3_PENALTY=0.379254
PRIMER_RIGHT_3_PENALTY=0.147383
PRIMER_LEFT_3_SEQUENCE=AAGAGGCCCTTGCTCTTCTTAC
PRIMER_RIGHT_3_SEQUENCE=TGCCTTAGTCAAAGCCGTTTAT
PRIMER_LEFT_3=261,22
PRIMER_RIGHT_3=472,22
PRIMER_LEFT_3_TM=60.379
PRIMER_RIGHT_3_TM=60.147
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.5266
PRIMER_LEFT_4_PENALTY=0.379254
PRIMER_RIGHT_4_PENALTY=0.147383
PRIMER_LEFT_4_SEQUENCE=AAGAGGCCCTTGCTCTTCTTAC
PRIMER_RIGHT_4_SEQUENCE=GCCTTAGTCAAAGCCGTTTATG
PRIMER_LEFT_4=261,22
PRIMER_RIGHT_4=471,22
PRIMER_LEFT_4_TM=60.379
PRIMER_RIGHT_4_TM=60.147
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_01872 Ps_contig_mira-and-tgicl-ass_franssen_17057
SEQUENCE=TTACCGATGCGGGAATGCGAGTCCAAGTCCGAGTCCGAGTCCGAAACCGGTTTTGAACACTGAAGCTGAAGAAAAAAGCTATCAAAGAAAAAGGGTTTTTCTTACACCCTTCACCTGGAAACACTCCAAGAGACTCTGAACTTCGTTTGCTTCCTCTGTTTCCGACTACTTCTCCTAGAGCTTCGGGTCCTTCTTCTTCTTCTACTTCGAGTACTTGAATTTTATTAAATTTTTAATTTTATTTTTAATTTTAATTCTTGGTGGTTTAGATTTTGTTTTAGATTTTTATAATCTATATTAATGGCATTGATGAGGTTGAGAAAGAGATAATTTGTTGTAATGGATTTTCACAGAATCGAAAATCTTGCTTCA
TARGET=190,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7377
PRIMER_LEFT_PENALTY=0.054021
PRIMER_RIGHT_PENALTY=0.683672
PRIMER_LEFT_SEQUENCE=CTGAACTTCGTTTGCTTCCTCT
PRIMER_RIGHT_SEQUENCE=TCTTTCTCAACCTCATCAATGC
PRIMER_LEFT=135,22
PRIMER_RIGHT=324,22
PRIMER_LEFT_TM=60.054
PRIMER_RIGHT_TM=59.316
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=0.7377
PRIMER_LEFT_1_PENALTY=0.054021
PRIMER_RIGHT_1_PENALTY=0.683672
PRIMER_LEFT_1_SEQUENCE=CTCTGAACTTCGTTTGCTTCCT
PRIMER_RIGHT_1_SEQUENCE=TCTTTCTCAACCTCATCAATGC
PRIMER_LEFT_1=133,22
PRIMER_RIGHT_1=324,22
PRIMER_LEFT_1_TM=60.054
PRIMER_RIGHT_1_TM=59.316
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.7554
PRIMER_LEFT_2_PENALTY=0.071684
PRIMER_RIGHT_2_PENALTY=0.683672
PRIMER_LEFT_2_SEQUENCE=GAACTTCGTTTGCTTCCTCTGT
PRIMER_RIGHT_2_SEQUENCE=TCTTTCTCAACCTCATCAATGC
PRIMER_LEFT_2=137,22
PRIMER_RIGHT_2=324,22
PRIMER_LEFT_2_TM=59.928
PRIMER_RIGHT_2_TM=59.316
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.7554
PRIMER_LEFT_3_PENALTY=0.071684
PRIMER_RIGHT_3_PENALTY=0.683672
PRIMER_LEFT_3_SEQUENCE=ACTCTGAACTTCGTTTGCTTCC
PRIMER_RIGHT_3_SEQUENCE=TCTTTCTCAACCTCATCAATGC
PRIMER_LEFT_3=132,22
PRIMER_RIGHT_3=324,22
PRIMER_LEFT_3_TM=59.928
PRIMER_RIGHT_3_TM=59.316
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.7830
PRIMER_LEFT_4_PENALTY=0.099308
PRIMER_RIGHT_4_PENALTY=0.683672
PRIMER_LEFT_4_SEQUENCE=CTTACACCCTTCACCTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=TCTTTCTCAACCTCATCAATGC
PRIMER_LEFT_4=100,22
PRIMER_RIGHT_4=324,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=59.316
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_01873 Ps_contig_mira-and-tgicl-ass_franssen_17060
SEQUENCE=ATCACTACTCCTTGATCATTCGTATCCAAAACTTCCCATTTCTTTGAATTCCGATAGAAGATGATGGAATATCTCTGTTAAAATCCAAACACTCGCCACCTTCAATCCAATCAATGACACGGTTAGATATTCGTTCCCTTCTCACATTCTCTGCAATTTTCCGCGTAATCTTAATCCTTTACGGAGAATGGCAAGATTCTCACATGGAAGTTAGATACACCGATGTTGATTACATCGTTTTTTCTGATGCCGCTTCTCTAGTCGCTTCTGGTTATTCTCCTTACAACAGAACCACCTACCGTTATTCTCCTTTACTCGCTTTTCTTCTTCTTCCTAATTCCTTTCTTCATCGCTCTTGGGGAAAGTTTCTCTTCTCTTCTGCAGATATACTTGTGGGATATTTCATCTATTACATTCTAAAGCTGCAGAAGGTTCCTGAMRACCT
TARGET=322,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01874 Ps_contig_mira-and-tgicl-ass_franssen_17062
SEQUENCE=AAACTTTTTTTTAACTTTTAACTACAATATTTTNTTAAATTATTAATANANTNNTAAATTTATAGTGAAAGAAATGAAATCTTTGATTAGATTATGCAATTGCTTTTGCTTGACGAGATCACGAACCTCAACCCCATGACAACCCCCTCGCCGTAAAACTCCTCACCACCACCACACCCTTCTCTTTCCATCACTCTCGCCACCACCATCTGATGGTCCATCAACGGATCCCCGTCACACCCACTCACCAGCACCCTCCGACCAAGCTCCCTCACCCTATCTAAAACCCCAACACCAACCGTTGGATTACAATACTCATGATCACGGTCRACACCAATCGGCAACGATAGTTCCCACATCAAATCGCACACATGCGGCGGCAAAACGGCATCGTTTATCAGCCTCAGCTCCGATGGTACTCTCTTGGTCCCACCGAAAAATGGTTGCGACAATATAAGCCCTCTAATATTAAGTGGATTTATATAATTGCGATTAAGATTAATCTCTGCAGCTACGCGCAGACCCGTGTGATAAGCGATATTTGCACCGGCGCTACCTCCCATGATGTAACAATTTGAATAATCAGCATAGTTTCTCAACCAAATGTCTGGTTTGGTTTTGAT
TARGET=164,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01875 Ps_contig_mira-and-tgicl-ass_franssen_17079
SEQUENCE=CGGGGGGAGGCAGKGAGATAATAGAGTCTCAAATTCTTCAAGGTTAAGKCCTCGTTTTTGTTTCTCTCTCTATATTTCTCTTTCTCTCTCATCAAGGTTGGAGATCTCATCTCCTCCACCACATCCACCACCACCACCACCACCACCACCATGCTCTTCAGATTCTCTCTCCTCCTGATTCTTCTCCTCTCTCCGGCCTTCGCTTCCGAGTCAGATCACAAGTATCAACAAGATGACCCAGTTACCCTTTGGGTGAACAAAGTAGGTCCTTATAATAATCCACAAGAAACATACAACTATTACAGCCTCCCATTTTGCCGTCCACCTGGTGATGCTCCTCACAAATGGGGTGGTCTAGGCGAGGTCCTTGGAGGCAATGAACTTATTGATAGTCAAATTGAGATAAAGTACCTAGGGAATGTGGAAAGGACCACTTTTTGCCGCTTGAATCTTGATGATGCAAAGGTTAAACAGTTCAAGGATGCAATAGAAAATAATTACTGGTTTGAATTCTTCATGGATGATCTTCCATTATGGGGGTATGTTGGAGAGTTGCATCCTGATAAGAA
TARGET=125,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01876 Ps_contig_mira-and-tgicl-ass_franssen_17080
SEQUENCE=AATTACATCAAATAGTAATTCTGGGGGTAAGCTTCGTCCCAGCAACTATTTTGAATAACAAGCGGCTGATCATGCAGATCCTGTACCGAACCATGAGATTTTCCCCTATGATGATGACCAGCAAGTTTGACATCAAAACTACGCCTAGATAAACTCCCAAAACTATCACGAACGTCACGAACTATGCTACGGAATGACATTCTGCCGGACTATCCCAAGTATCTCTTCTGCAAACTTCTGATGGATTCTATATCCAACCACTGTGAGCATATATATGACTCTAAAAAACTTGTAAATTAACCTCACTTCATAAAAAACCCCAAGCTAGATCACAATCCACCTTACAAGTTAATCGAGAATGTTTAGATCTAAAGTAGAAGAGTTGTATTGAATGAAGCTGAAATGAAAGATACAATCTTGCAACAAAATAGAAAGATATATACTTTAGGTCAGAAAATTGCTAACATCCTCAGTTTCTTACACCTCCCTCATCTCTCTCTCTCTCTCTCTCTTGTTNTCTCTCTCTCTCTCTCTNTCTCCG
TARGET=492,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3103
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=2.309542
PRIMER_LEFT_SEQUENCE=AAAACCCCAAGCTAGATCACAA
PRIMER_RIGHT_SEQUENCE=CGGAGANAGAGAGAGAGAGAGAGA
PRIMER_LEFT=313,22
PRIMER_RIGHT=540,24
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.690
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=52.174
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=2.3103
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=2.309542
PRIMER_LEFT_1_SEQUENCE=AAAAACCCCAAGCTAGATCACA
PRIMER_RIGHT_1_SEQUENCE=CGGAGANAGAGAGAGAGAGAGAGA
PRIMER_LEFT_1=312,22
PRIMER_RIGHT_1=540,24
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.690
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=52.174
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=229
PRIMER_PAIR_PENALTY_2=2.8227
PRIMER_LEFT_2_PENALTY=0.513182
PRIMER_RIGHT_2_PENALTY=2.309542
PRIMER_LEFT_2_SEQUENCE=AACCCCAAGCTAGATCACAATC
PRIMER_RIGHT_2_SEQUENCE=CGGAGANAGAGAGAGAGAGAGAGA
PRIMER_LEFT_2=315,22
PRIMER_RIGHT_2=540,24
PRIMER_LEFT_2_TM=59.487
PRIMER_RIGHT_2_TM=59.690
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.174
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=2.75
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=3.1282
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=3.127492
PRIMER_LEFT_3_SEQUENCE=AAAACCCCAAGCTAGATCACAA
PRIMER_RIGHT_3_SEQUENCE=CGGAGANAGAGAGAGAGAGAGAG
PRIMER_LEFT_3=313,22
PRIMER_RIGHT_3=540,23
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=57.873
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=3.1282
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=3.127492
PRIMER_LEFT_4_SEQUENCE=AAAAACCCCAAGCTAGATCACA
PRIMER_RIGHT_4_SEQUENCE=CGGAGANAGAGAGAGAGAGAGAG
PRIMER_LEFT_4=312,22
PRIMER_RIGHT_4=540,23
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=57.873
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_01877 Ps_contig_mira-and-tgicl-ass_franssen_17080
SEQUENCE=AATTACATCAAATAGTAATTCTGGGGGTAAGCTTCGTCCCAGCAACTATTTTGAATAACAAGCGGCTGATCATGCAGATCCTGTACCGAACCATGAGATTTTCCCCTATGATGATGACCAGCAAGTTTGACATCAAAACTACGCCTAGATAAACTCCCAAAACTATCACGAACGTCACGAACTATGCTACGGAATGACATTCTGCCGGACTATCCCAAGTATCTCTTCTGCAAACTTCTGATGGATTCTATATCCAACCACTGTGAGCATATATATGACTCTAAAAAACTTGTAAATTAACCTCACTTCATAAAAAACCCCAAGCTAGATCACAATCCACCTTACAAGTTAATCGAGAATGTTTAGATCTAAAGTAGAAGAGTTGTATTGAATGAAGCTGAAATGAAAGATACAATCTTGCAACAAAATAGAAAGATATATACTTTAGGTCAGAAAATTGCTAACATCCTCAGTTTCTTACACCTCCCTCATCTCTCTCTCTCTCTCTCTCTTGTTNTCTCTCTCTCTCTCTCTNTCTCCG
TARGET=518,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01878 Ps_contig_mira-and-tgicl-ass_franssen_17086
SEQUENCE=GGGAAGGCAATTCAAAATGAAAATGAATTAAAACTCAACGTGAAATACAAAAATAACCAAGCGCAGCAAGAGAACAAGGAGTGAGTTGCAAATTGGAAGCTGCAAACTGCAGCGCGAGTTGCATGCCAATTGCAGAACGCCGATGATGAACATCACAACTGAAATCGCAGCTACATCAGTAGGAATGAAGCACACCAGCGGCAACTTCGGAAAACGCAGCAAACTCACAAGCTACACATAAGCAAAGCCGTAACACCATCAAAGTCAAGGCAACATGAAAACGCAAAGGTGAAAAGCGACACCTGCATAACTTCGAAAGTGAAGTCCCTGCTAACAGCTAACCAACTCACAACTGACGGTTAACCAAACGAAAAAATCTGAAAACGACCACCGTTAACGTCACTGGCGTTCCAATCGTATTCTCCGGCGAGTTCATTGGTTGCTCCAGTCCAATTCGCGATCCAATCAACCGTCCGCTCATGCACGAAATCATCATCATCCTGCTTCAATATCCTCTCCCCGATATCACGCACGCCCAAACATGTGGATTCAGCTTCCGTCACCGCCGTGTCCATCATTGTCTCCGGTGGTAAGTGCACTTTCATCTTTGCTTCACCTTATTTCAAGTTACCACGATGTAGGTGAAGGCTTAAGGATTTAAAGCCCCATTGAATTATCCTGCGCGTGTTAGGGATTTCGTTTAGAATGATTCTGTTAAGTTGATTTAGCACGAKTTGTTGAGTTTTGTTTATGGATTTGGTTAGGGTTTGTTGAGGCGAGTTCGGTATTGCAGGTATTACGGTGTTTGGCTCTCCACCGCAGCCGGAGGCGATTTCCAGCGCGACGGAGATCTACTTTGGTCAAGGCTTCGTTCTGAGCTTGTGAGGCATTGAGTGGAGCTGAACCCCTCCCTTCSTTC
TARGET=489,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01879 Ps_contig_mira-and-tgicl-ass_franssen_17104
SEQUENCE=ATGATAAACAATTAANAAAACAATNTAGTAACTCTGCTCACAACAATCACAGATCATAATACCTACTACAACAATTAAAACGAAATAAAACTCAAAAATRAAGGTTAAAAAGTAAAAATAMCTAATAAGTAGCAGAGAGAAAGATAAGATTCAAGCCTGTGTTCCAGCCAAAGCTTTCATCTGAATATGATTTGATTCCGTGGGCGAAGACGTAGCAGTAGGCAATGCCTCGTAGGATCCAACCCTTCTAACAGTCCCAACAGTAGTGGCAACAGCAGCATAAGCAACCAAAACAATGATCATAACCAACATAACATGAACAACAAAAGCTAGATCAAGAATAGCAAGCGCTCTAAACTTAGACTCGTCAAGATCACACTGCGTCGATCCTTCGACGCCGTTAACAACGTCGAGGAGACGATGACAGCCTTCCGGGATAAAGGCTTCGGCGTAAAGAGAAAGACCGGTTTGAAGAGCCCATAGGCCACGGAGACAGATGGATGCAGCGAGTGCGGCGTCGGCGGGGTAGAGACGAGGGAAACATGCGAGGAGGAGACAGAGGAAGGAAGAGAGAGCGGAGAGATTGGAAGAAACGGAGTCGCATTTGGCTTCGAGGGAGGAGGTTTGGAGAGAGGAGAGGGAACTTTGGAGGGAGGAGTGGAGGAGGAAGAGGAGGGCAAGGAGAGAGAAAGAGAAGTCGGGGGAGAGAGGGAGAGGGAGGAGGAGAGAGAGGGAGAGGATTAAGAAGAGGAAAGGAGGGCAGCGGATTGGAGAGAAGTGAGACGGTGGACGGGGGTGGCGCCTTTGATTAAAGGATCGGAATCAGCGGCGAGGATGAGGTGGTGGACGATGGCGATGGAGAGAGAGAAAATGATGAAGTAGAGTTGGATAGGGCGGAGACGAGGGTGGTTGGAGGACGGGAGAGGGTGGTAGGGTTTGGATGTGTAGGTGTGGGGGGATTTGAAGTAGTTTAGGGTTGTGAGAATGAGGTGGTAGAGACCTAATGAAAGGAGTGCTGAGGATGATAATGCTTGGTATAATAAACTTCCCATTTTGATTTTGATGGTGGCGGTGGTGGTGATGATGGAGCTACTACTGTTTTTTGAAGTAGTAACGAGGAGAAGAGATTATGATTGGTGGATTGATTATTATTTTGTTCTTTCTTTCTTTTTGGTCAAGAGAAACAAACAAGAGAAAGAGAGGATGGATCATTTTTTTGGATCCTCATTTTCTTTCCATCTACTTTGTTCTCCTC
TARGET=859,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01880 Ps_contig_mira-and-tgicl-ass_franssen_17104
SEQUENCE=ATGATAAACAATTAANAAAACAATNTAGTAACTCTGCTCACAACAATCACAGATCATAATACCTACTACAACAATTAAAACGAAATAAAACTCAAAAATRAAGGTTAAAAAGTAAAAATAMCTAATAAGTAGCAGAGAGAAAGATAAGATTCAAGCCTGTGTTCCAGCCAAAGCTTTCATCTGAATATGATTTGATTCCGTGGGCGAAGACGTAGCAGTAGGCAATGCCTCGTAGGATCCAACCCTTCTAACAGTCCCAACAGTAGTGGCAACAGCAGCATAAGCAACCAAAACAATGATCATAACCAACATAACATGAACAACAAAAGCTAGATCAAGAATAGCAAGCGCTCTAAACTTAGACTCGTCAAGATCACACTGCGTCGATCCTTCGACGCCGTTAACAACGTCGAGGAGACGATGACAGCCTTCCGGGATAAAGGCTTCGGCGTAAAGAGAAAGACCGGTTTGAAGAGCCCATAGGCCACGGAGACAGATGGATGCAGCGAGTGCGGCGTCGGCGGGGTAGAGACGAGGGAAACATGCGAGGAGGAGACAGAGGAAGGAAGAGAGAGCGGAGAGATTGGAAGAAACGGAGTCGCATTTGGCTTCGAGGGAGGAGGTTTGGAGAGAGGAGAGGGAACTTTGGAGGGAGGAGTGGAGGAGGAAGAGGAGGGCAAGGAGAGAGAAAGAGAAGTCGGGGGAGAGAGGGAGAGGGAGGAGGAGAGAGAGGGAGAGGATTAAGAAGAGGAAAGGAGGGCAGCGGATTGGAGAGAAGTGAGACGGTGGACGGGGGTGGCGCCTTTGATTAAAGGATCGGAATCAGCGGCGAGGATGAGGTGGTGGACGATGGCGATGGAGAGAGAGAAAATGATGAAGTAGAGTTGGATAGGGCGGAGACGAGGGTGGTTGGAGGACGGGAGAGGGTGGTAGGGTTTGGATGTGTAGGTGTGGGGGGATTTGAAGTAGTTTAGGGTTGTGAGAATGAGGTGGTAGAGACCTAATGAAAGGAGTGCTGAGGATGATAATGCTTGGTATAATAAACTTCCCATTTTGATTTTGATGGTGGCGGTGGTGGTGATGATGGAGCTACTACTGTTTTTTGAAGTAGTAACGAGGAGAAGAGATTATGATTGGTGGATTGATTATTATTTTGTTCTTTCTTTCTTTTTGGTCAAGAGAAACAAACAAGAGAAAGAGAGGATGGATCATTTTTTTGGATCCTCATTTTCTTTCCATCTACTTTGTTCTCCTC
TARGET=1157,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01881 Ps_contig_mira-and-tgicl-ass_franssen_17106
SEQUENCE=GGGGGGCGTGAATAGTCAAATCCTCCAAACTAGCAACGTATCATATTTCCCTTCCATTTCTTTCATTGTCGGCGCAATTCATTTCTAAAGACGCGTTCTTCTCTAACTTCATTCATTCATTCACACTCCAACCTAATTTCTCTGATTTCACGATCATTCTCAACACAGATTTTAGTTCGATTGCGATGGCTAGAAAGATGCTTAACGATGGTGAACCTGATTTGAAGAAAGGTGAAGAACAAGGGAAGGTGTATGATTTTGATTTGTTTATTATTGGAGCTGGTAGTGGCGGTGTTCGTGCTGCTAGATTTTCATCTAATTTTGGAGCAAAGGTTGGTATTTGTGAGCTTCCTTTTCATCCTATTAGCTCGGAAACAATCGGAGGAGTCGGCGGCACGTGTGTCATTCGCGGTTGTGTTCCCAAAAAGATTTTAGTCTATGGAGCATCTTATGGAGGTGAACTTCAGGATGCTAGAAATTTTGGATGGGAATTGAATGAGAATGTTGACTTCAATTGGAAGAAGCTCTTGCAAAAGAAGACAGATGAAATAAACAGATTAAATGGAATTTACAAGCGGTTGTTATCCAATGCTGGGGTTAAACTATTTGAAGGTGAGGGAAAGATAGCTAGTCCAAATGAAGTTGAGGTGACCCAATTGGATGGCACTAAGTTGTCGTATTCAGCAAAGCATATTTTGATTGCAACTGGCAGCAGGGCTCAGCGTCCTAATATTCCTGGCCAGGAGTTGGGTATAACATCTGATGAGGCACTAAGTTTAGAGGAGTTTCCTAAACGTGCTGTGATCCTCGGAGGAGGTTATATTGCAGTTGAGTTTGCATCTATATGGCGTGGGATGGGTTCCAGTGTCAATCTTGTTTTCAGAAAGGAACTTCCATTGAGAGGTTTTGATGACGAAATGAGAGCTGTAGTTGCCAGAAACCTTGAAGGTAGGGGAATTAATTTGCATCCAAGGACAAATTTGGCACAGTTGATCAAAACAGAGGATGGCATTAAAGTTATTACAGACCATGGTGAGGAGTTGATTGCGGATGTTGTGCTATTTGCCACTGGTAGGTCTCCTAATTCGAAGAGGTTAAATTTAGAAAAAGTTGGTGTAGAGTTTGATAAGGCAGGAGCAATAGTGGTAGATGAGTATTCACGCACCAACATCCCTAGCATATGGGCTGTGGGTGATGTAACCAACCGATTGAATCTTACCCCGGTGGCCTTGATGGAAGCATCACTCTTTGCAAAAACAGTATTTGGTGGGCAAGCAAGCAAGCCAGACTATAATGATATTCCATATGCAGTGTTTTGCATTCCACCACTTTCAGTAGTTGGTCTAAGTGAGGAGCAGGCAGTAGAGCAGACAAAGGGTGATGTATTGATTTTCACATCAACCTTCAATCCTATGAAAAATACAATTTCTGGGCGGCAAGAAAAAACTGTTATGAAGCTTGTTGTTGATGCTCAGACCGATAAGGTTCTTGGAGCATCTATGTGTGGGCCTGATGCACCTGAAATTGTTCAGGGTATTGCTATTGCAATTAAGTGTGGAGCTACGAAAGCGCAGTTTGATAGCACCGTGGGAATACACCCATCGTCTGCAGAGGAATTTGTGACCATGCGCTCGGAGACAAGGCGTGTTACAGGTGGTGTGAAGCCAAAGACAAATTTGTAAACGCGTTTTGATTAGTTATTTAAATAGAGAATAAAATCAAGTCACATTGGTCAGGACTTACGTGCATGTTTCTTTTCTTGGGCATATAGCCCCTTCGATTTTGTAAAATCATCTAAATATAAACTATGTTGTTAATGGTGTAGCTGCAATACTAGAACAACCAAAGAGTATTCTTTTATAGCAAATTTCCTATTTTTGAAAACTTTGTAATGAAGATGTTGGCTCATTCTTCCCGTCTGATTTCATCAGAGTCTTCCTAACAATAATTTGGTAATGCCAAAGAAATTGTGTGACT
TARGET=108,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1046
PRIMER_LEFT_PENALTY=0.101938
PRIMER_RIGHT_PENALTY=0.002623
PRIMER_LEFT_SEQUENCE=GGCGCAATTCATTTCTAAAGAC
PRIMER_RIGHT_SEQUENCE=CCAACCTTTGCTCCAAAATTAG
PRIMER_LEFT=70,22
PRIMER_RIGHT=336,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=59.997
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.1884
PRIMER_LEFT_1_PENALTY=0.185822
PRIMER_RIGHT_1_PENALTY=0.002623
PRIMER_LEFT_1_SEQUENCE=CCCTTCCATTTCTTTCATTGTC
PRIMER_RIGHT_1_SEQUENCE=CCAACCTTTGCTCCAAAATTAG
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=336,22
PRIMER_LEFT_1_TM=59.814
PRIMER_RIGHT_1_TM=59.997
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=289
PRIMER_PAIR_PENALTY_2=0.3226
PRIMER_LEFT_2_PENALTY=0.101938
PRIMER_RIGHT_2_PENALTY=0.220659
PRIMER_LEFT_2_SEQUENCE=GGCGCAATTCATTTCTAAAGAC
PRIMER_RIGHT_2_SEQUENCE=CGCAATCGAACTAAAATCTGTG
PRIMER_LEFT_2=70,22
PRIMER_RIGHT_2=184,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=59.779
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=115
PRIMER_PAIR_PENALTY_3=0.3435
PRIMER_LEFT_3_PENALTY=0.101938
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=GGCGCAATTCATTTCTAAAGAC
PRIMER_RIGHT_3_SEQUENCE=AGGATGAAAAGGAAGCTCACAA
PRIMER_LEFT_3=70,22
PRIMER_RIGHT_3=361,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=292
PRIMER_PAIR_PENALTY_4=0.4065
PRIMER_LEFT_4_PENALTY=0.185822
PRIMER_RIGHT_4_PENALTY=0.220659
PRIMER_LEFT_4_SEQUENCE=CCCTTCCATTTCTTTCATTGTC
PRIMER_RIGHT_4_SEQUENCE=CGCAATCGAACTAAAATCTGTG
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=184,22
PRIMER_LEFT_4_TM=59.814
PRIMER_RIGHT_4_TM=59.779
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_01882 Ps_contig_mira-and-tgicl-ass_franssen_17106
SEQUENCE=GGGGGGCGTGAATAGTCAAATCCTCCAAACTAGCAACGTATCATATTTCCCTTCCATTTCTTTCATTGTCGGCGCAATTCATTTCTAAAGACGCGTTCTTCTCTAACTTCATTCATTCATTCACACTCCAACCTAATTTCTCTGATTTCACGATCATTCTCAACACAGATTTTAGTTCGATTGCGATGGCTAGAAAGATGCTTAACGATGGTGAACCTGATTTGAAGAAAGGTGAAGAACAAGGGAAGGTGTATGATTTTGATTTGTTTATTATTGGAGCTGGTAGTGGCGGTGTTCGTGCTGCTAGATTTTCATCTAATTTTGGAGCAAAGGTTGGTATTTGTGAGCTTCCTTTTCATCCTATTAGCTCGGAAACAATCGGAGGAGTCGGCGGCACGTGTGTCATTCGCGGTTGTGTTCCCAAAAAGATTTTAGTCTATGGAGCATCTTATGGAGGTGAACTTCAGGATGCTAGAAATTTTGGATGGGAATTGAATGAGAATGTTGACTTCAATTGGAAGAAGCTCTTGCAAAAGAAGACAGATGAAATAAACAGATTAAATGGAATTTACAAGCGGTTGTTATCCAATGCTGGGGTTAAACTATTTGAAGGTGAGGGAAAGATAGCTAGTCCAAATGAAGTTGAGGTGACCCAATTGGATGGCACTAAGTTGTCGTATTCAGCAAAGCATATTTTGATTGCAACTGGCAGCAGGGCTCAGCGTCCTAATATTCCTGGCCAGGAGTTGGGTATAACATCTGATGAGGCACTAAGTTTAGAGGAGTTTCCTAAACGTGCTGTGATCCTCGGAGGAGGTTATATTGCAGTTGAGTTTGCATCTATATGGCGTGGGATGGGTTCCAGTGTCAATCTTGTTTTCAGAAAGGAACTTCCATTGAGAGGTTTTGATGACGAAATGAGAGCTGTAGTTGCCAGAAACCTTGAAGGTAGGGGAATTAATTTGCATCCAAGGACAAATTTGGCACAGTTGATCAAAACAGAGGATGGCATTAAAGTTATTACAGACCATGGTGAGGAGTTGATTGCGGATGTTGTGCTATTTGCCACTGGTAGGTCTCCTAATTCGAAGAGGTTAAATTTAGAAAAAGTTGGTGTAGAGTTTGATAAGGCAGGAGCAATAGTGGTAGATGAGTATTCACGCACCAACATCCCTAGCATATGGGCTGTGGGTGATGTAACCAACCGATTGAATCTTACCCCGGTGGCCTTGATGGAAGCATCACTCTTTGCAAAAACAGTATTTGGTGGGCAAGCAAGCAAGCCAGACTATAATGATATTCCATATGCAGTGTTTTGCATTCCACCACTTTCAGTAGTTGGTCTAAGTGAGGAGCAGGCAGTAGAGCAGACAAAGGGTGATGTATTGATTTTCACATCAACCTTCAATCCTATGAAAAATACAATTTCTGGGCGGCAAGAAAAAACTGTTATGAAGCTTGTTGTTGATGCTCAGACCGATAAGGTTCTTGGAGCATCTATGTGTGGGCCTGATGCACCTGAAATTGTTCAGGGTATTGCTATTGCAATTAAGTGTGGAGCTACGAAAGCGCAGTTTGATAGCACCGTGGGAATACACCCATCGTCTGCAGAGGAATTTGTGACCATGCGCTCGGAGACAAGGCGTGTTACAGGTGGTGTGAAGCCAAAGACAAATTTGTAAACGCGTTTTGATTAGTTATTTAAATAGAGAATAAAATCAAGTCACATTGGTCAGGACTTACGTGCATGTTTCTTTTCTTGGGCATATAGCCCCTTCGATTTTGTAAAATCATCTAAATATAAACTATGTTGTTAATGGTGTAGCTGCAATACTAGAACAACCAAAGAGTATTCTTTTATAGCAAATTTCCTATTTTTGAAAACTTTGTAATGAAGATGTTGGCTCATTCTTCCCGTCTGATTTCATCAGAGTCTTCCTAACAATAATTTGGTAATGCCAAAGAAATTGTGTGACT
TARGET=1271,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1002
PRIMER_LEFT_PENALTY=0.018835
PRIMER_RIGHT_PENALTY=0.081328
PRIMER_LEFT_SEQUENCE=TGCCACTGGTAGGTCTCCTAAT
PRIMER_RIGHT_SEQUENCE=ACTGCCTGCTCCTCACTTAGAC
PRIMER_LEFT=1063,22
PRIMER_RIGHT=1362,22
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.2769
PRIMER_LEFT_1_PENALTY=0.195574
PRIMER_RIGHT_1_PENALTY=0.081328
PRIMER_LEFT_1_SEQUENCE=AAGGCAGGAGCAATAGTGGTAG
PRIMER_RIGHT_1_SEQUENCE=ACTGCCTGCTCCTCACTTAGAC
PRIMER_LEFT_1=1127,22
PRIMER_RIGHT_1=1362,22
PRIMER_LEFT_1_TM=59.804
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.3208
PRIMER_LEFT_2_PENALTY=0.018835
PRIMER_RIGHT_2_PENALTY=0.301926
PRIMER_LEFT_2_SEQUENCE=TGCCACTGGTAGGTCTCCTAAT
PRIMER_RIGHT_2_SEQUENCE=CCTGCTCCTCACTTAGACCAAC
PRIMER_LEFT_2=1063,22
PRIMER_RIGHT_2=1358,22
PRIMER_LEFT_2_TM=60.019
PRIMER_RIGHT_2_TM=60.302
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=0.3302
PRIMER_LEFT_3_PENALTY=0.248900
PRIMER_RIGHT_3_PENALTY=0.081328
PRIMER_LEFT_3_SEQUENCE=TTGATGGAAGCATCACTCTTTG
PRIMER_RIGHT_3_SEQUENCE=ACTGCCTGCTCCTCACTTAGAC
PRIMER_LEFT_3=1229,22
PRIMER_RIGHT_3=1362,22
PRIMER_LEFT_3_TM=60.249
PRIMER_RIGHT_3_TM=60.081
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=134
PRIMER_PAIR_PENALTY_4=0.3513
PRIMER_LEFT_4_PENALTY=0.269968
PRIMER_RIGHT_4_PENALTY=0.081328
PRIMER_LEFT_4_SEQUENCE=GTGATGTAACCAACCGATTGAA
PRIMER_RIGHT_4_SEQUENCE=ACTGCCTGCTCCTCACTTAGAC
PRIMER_LEFT_4=1191,22
PRIMER_RIGHT_4=1362,22
PRIMER_LEFT_4_TM=59.730
PRIMER_RIGHT_4_TM=60.081
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_01883 Ps_contig_mira-and-tgicl-ass_franssen_17123
SEQUENCE=TACCAAAAGTAGGAAGTWGAAATACGACAACGAACTCACACCATAAGCACCAAAGTTAATCATCGTAACGGGAAGGAAACAACTTAACCGGCGGATGTGACGGGAGGCAACGGCGACAGTTGAGAAATTCCGTATGCTTTCACTCCTCCGTTTTATCCTCCGTCTTCTCTTCTTCTGTTTATCACCTCCGAGAATTGAGAGAATTGTTGTGGAATGGTTGCTTCGGCTAATCGGTTGTGATGTGATTCGCAAAGTCGTGGTGACGTCGTATTGCCGAAGAATTGAAGAATGTAGAAGATGTCGCGGTGAGCTTGGCATGGTTGTTGTAATCCGTGTGCGGAGGTGATTGAAGTGGTTGAGGCGGTGTTGATGAAGGATTGAGGAGAAGATTCTCGAAGCCGATCACCTCTTTTGTGGGTTCAAGAAGAAGATTGAAGGTTGTTTGAGCATGGTGAAGGTTGAGAAAAACGGCTATGGAAGATTGAAGAAGAAAGAGAAGAGGAGTTTGTTAGAAACGGGAGAGGAAGAAGAAGAATCTTCAAAGCCTTTGTCTCAATTTATAGAGTTATGGGATTCGAACTTCTGCTGCGTTATCATTTAGTTGTCGTTTGGCTTATGTATCCGTTTTGAATTTCCATTTCACTTGTCGTTTACAATTTTCAGTTGCGTTTCAATTATGTATGATGTGTGTTCATGTCGTTTGGATTAGCTTGCGTTTTCTGCTACTGTGATTCAACTTTTCATTGAGCGCGTTTTGGCATCACAGT
TARGET=524,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01884 Ps_contig_mira-and-tgicl-ass_franssen_17125
SEQUENCE=CCTGCTTCTTTTTAACCTCCTGACAATGGCAACAGGGTTTTTTCTAAAGCCCACGGATGAGCAAGAACGGAGACACCCCCGGTATCGCTAATCATTTTTATTGCTTCCTCCACATCAGGTTCACTTCCCTTGGAGTAAGCAGGTCCACCATCAAAAAGATATCGGGCAAAAGCTTGTTTGAGATTTTCAACGTAACCTGCTTCAACCATAGCACGGGCTACATGGAGCCTTCCGGGAGCAACTCCGTTGCCTGCAATCCTACAAACTTGCTCCCACTTAAGAGCCATTTTTAGCTTGTTCAGTTTTAAGATCATGTTCTTAGCTCGAAGGTAACGTCCGTCCCTTATGTTTGACAAAAACTTGTCCAGYCATTTTAAACTTTGAAGGTCCAATACTGCTATAATATGCTAGAATGTGAACAGGTTCCTCTCCTTCAGTGTCTGTGTCTCCACTAGGAGAATATATTGTACTAATTTCAACACCCGGGATTATCTTGATGCCATATTTACGAGCCGATTCTACAGCCTCAGGGATGCCTGCCATAGTGTCATGATCTGTTAGAGCAAGAACTTTCACGCCATTCATGTGAGCTCTCTCAACAACCTTAGAAGGAGACAAAAACCCATCACTACATTTAGAGTGCGAGTGCAATTCAAAGAGGACTCTGTCACCATTTTTCCCAACAGGCTTCTGTACCCCAAAATCATCGACAAGAGATGAAGGAGCCAAAGCCAAAGAAGAAGAAGGGTGTTCCAGAAAAACCCATTCAGTGACCGACTTAAAAGCCAAAACTTGTTCGTGGGTCATCTTCTTCTTGCTTCCACCTCTATTCTTCTTCTTCTTCTTTTTCTTATCCTTATTACCCTTAATCGAATTACTTGTGCTCTGAACTGCGATACCATCTCCCATAACTGGATTCAGTTTTTTGCCTAAAGATTAAATTTTGAATTTTTGTTAGGTCATTTTAGGGTGAGTTTGTTAGAGTACAGATGCTTCTATATAACACGGGAAGAAGAAAAAACATAAGAGGGAAAGAGAAAGGGTACCTGACCCTTATACTGAGTGTCACAGGTGTCCTCC
TARGET=735,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01885 Ps_contig_mira-and-tgicl-ass_franssen_17125
SEQUENCE=CCTGCTTCTTTTTAACCTCCTGACAATGGCAACAGGGTTTTTTCTAAAGCCCACGGATGAGCAAGAACGGAGACACCCCCGGTATCGCTAATCATTTTTATTGCTTCCTCCACATCAGGTTCACTTCCCTTGGAGTAAGCAGGTCCACCATCAAAAAGATATCGGGCAAAAGCTTGTTTGAGATTTTCAACGTAACCTGCTTCAACCATAGCACGGGCTACATGGAGCCTTCCGGGAGCAACTCCGTTGCCTGCAATCCTACAAACTTGCTCCCACTTAAGAGCCATTTTTAGCTTGTTCAGTTTTAAGATCATGTTCTTAGCTCGAAGGTAACGTCCGTCCCTTATGTTTGACAAAAACTTGTCCAGYCATTTTAAACTTTGAAGGTCCAATACTGCTATAATATGCTAGAATGTGAACAGGTTCCTCTCCTTCAGTGTCTGTGTCTCCACTAGGAGAATATATTGTACTAATTTCAACACCCGGGATTATCTTGATGCCATATTTACGAGCCGATTCTACAGCCTCAGGGATGCCTGCCATAGTGTCATGATCTGTTAGAGCAAGAACTTTCACGCCATTCATGTGAGCTCTCTCAACAACCTTAGAAGGAGACAAAAACCCATCACTACATTTAGAGTGCGAGTGCAATTCAAAGAGGACTCTGTCACCATTTTTCCCAACAGGCTTCTGTACCCCAAAATCATCGACAAGAGATGAAGGAGCCAAAGCCAAAGAAGAAGAAGGGTGTTCCAGAAAAACCCATTCAGTGACCGACTTAAAAGCCAAAACTTGTTCGTGGGTCATCTTCTTCTTGCTTCCACCTCTATTCTTCTTCTTCTTCTTTTTCTTATCCTTATTACCCTTAATCGAATTACTTGTGCTCTGAACTGCGATACCATCTCCCATAACTGGATTCAGTTTTTTGCCTAAAGATTAAATTTTGAATTTTTGTTAGGTCATTTTAGGGTGAGTTTGTTAGAGTACAGATGCTTCTATATAACACGGGAAGAAGAAAAAACATAAGAGGGAAAGAGAAAGGGTACCTGACCCTTATACTGAGTGTCACAGGTGTCCTCC
TARGET=830,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01886 Ps_contig_mira-and-tgicl-ass_franssen_17150
SEQUENCE=GGAGAGTCAGCTTCTTTGTTTGTTAGAGAGAGAAAATAAAAAGGTTTTTAGAGAGAGAAAGTGGGTCTTGTTTAAACACAGACAGAGGATCTAGGAGCGTGTTCTTTGATAAGACTTTGAAACATCATGATCTCTTATGTTCTTTGATCTCAGCGTTTTGGAGTGTTTGAGAAGCTATTCACTCTAGTGGAAGAAAATTTATTTATTCTTGGAGCGGCTTTGGTGATATATATATACTAGGTTTTAAGGTTGTCAGGATGAATTGCACT
TARGET=226,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9473
PRIMER_LEFT_PENALTY=0.038090
PRIMER_RIGHT_PENALTY=0.909234
PRIMER_LEFT_SEQUENCE=AGACAGAGGATCTAGGAGCGTG
PRIMER_RIGHT_SEQUENCE=GCAATTCATCCTGACAACCTTA
PRIMER_LEFT=79,22
PRIMER_RIGHT=265,22
PRIMER_LEFT_TM=60.038
PRIMER_RIGHT_TM=59.091
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.9473
PRIMER_LEFT_1_PENALTY=0.038090
PRIMER_RIGHT_1_PENALTY=0.909234
PRIMER_LEFT_1_SEQUENCE=CAGACAGAGGATCTAGGAGCGT
PRIMER_RIGHT_1_SEQUENCE=GCAATTCATCCTGACAACCTTA
PRIMER_LEFT_1=78,22
PRIMER_RIGHT_1=265,22
PRIMER_LEFT_1_TM=60.038
PRIMER_RIGHT_1_TM=59.091
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=188
PRIMER_PAIR_PENALTY_2=0.9473
PRIMER_LEFT_2_PENALTY=0.038090
PRIMER_RIGHT_2_PENALTY=0.909234
PRIMER_LEFT_2_SEQUENCE=ACAGACAGAGGATCTAGGAGCG
PRIMER_RIGHT_2_SEQUENCE=GCAATTCATCCTGACAACCTTA
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=265,22
PRIMER_LEFT_2_TM=60.038
PRIMER_RIGHT_2_TM=59.091
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=1.0021
PRIMER_LEFT_3_PENALTY=0.092913
PRIMER_RIGHT_3_PENALTY=0.909234
PRIMER_LEFT_3_SEQUENCE=GACAGAGGATCTAGGAGCGTGT
PRIMER_RIGHT_3_SEQUENCE=GCAATTCATCCTGACAACCTTA
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=265,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=59.091
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=1.0810
PRIMER_LEFT_4_PENALTY=0.171757
PRIMER_RIGHT_4_PENALTY=0.909234
PRIMER_LEFT_4_SEQUENCE=ATCTCAGCGTTTTGGAGTGTTT
PRIMER_RIGHT_4_SEQUENCE=GCAATTCATCCTGACAACCTTA
PRIMER_LEFT_4=146,22
PRIMER_RIGHT_4=265,22
PRIMER_LEFT_4_TM=60.172
PRIMER_RIGHT_4_TM=59.091
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_01887 Ps_contig_mira-and-tgicl-ass_franssen_17157
SEQUENCE=GTTCGTTTGTTACTTATTTTCTTATTGTCTTTCTTCTAACCYTTCATTTTCATTCCCATCTCAATCTCTCACCATTCACAACCCAACACTAAAACCATTACCCTCAACTTCACTTTCTATCCATTATCATCATCCAGTTCATGCCCAACAACACCAGAGCCAACTACATCATGATAATGATGAAGAAGAAGAAGAAAGCTTTCAAATTCTAACATCTGTGAAAACCGATTACAATGATATCATGATTGTGGATACCCCTAATTCTAGAATGCTTCTCCTTGATTCATCTCATAATGTTCATAGCATTCTTTACAAGGAGCGAAAATGGACAAATTCGTACTGGGACGAATTTGCCAGCTTGCCAGCCATTGTTCCAGAAGGCCCTATTGCTATCTTAGGTTTGGGTGGTGGAACTGCTGCTCATCTGATGCTTGAATTGTGGCCTTCGCTGCGACTTGAAGGATGGGAGATTGACGACATTTTGATCGATAAAGCAAGAGATTACTTTGGTTTATCTGATCTGGAGAAGACAACAGAAGATGGTGGCATTCTAAGTGTTCATATTGGCGATGTCTTCGTTCCCTCAGAGGATTTGCACGGAAGATACGCGGGAATTGTTGTTGACTTGTTCTCTGATGGAAAAGTTTTGCCTCAACTGCAAGAGGTTAGTACATGGTTGGAATTACGGGATCGATTGATGGCCAATGGTCGTTTGATGGTGAACTGTGGTGGCATTGACGCGAGTTCAAGTGTCGCATTTGCAAGTAGTGATCCTGGAAATTTATCAAATGATGAAGCATGGTTATTAAATGCAGCAATGAAAGCGTTATCCAAAGCATTTCCTGGAGAACTAAGTTGGAAGAGAATGCCGAAGGAAACCGGTGAGAATTTTATGGCACTTACAGGAGCCTTGCCTGATCTGAAATCTTGGTCTGCTAATGTCCCGTCTCCTTTGAGCACAGGAGTGGTGGATTGGAGACCTTGTGGAATGGTTTCTAGAATTTGATCTTTACCACTTCATGGTTAATGTGGAAATATGTTTTTGTAGGGAATGAACAGAGCAGAATAATAAGGAGCAGAGTAGAATGAAACAAAATGGATTGAATCGGAATGAATCATCTCAAAATTTAAAGATGGAATAAGATAAATTATTCATTCCGTCCAAATCGAAGGGGAAGAAATATGATGATAAATGATGGAATGGATGCCCTGTTCCATTCCATCTAATTTTAAATGATTCAAATAATAGAATACCATTTCATTTTATCTTATAGTTTCATCTGATTCTATCAATCCAAAACAAATTCTTAGAGTATAGAA
TARGET=182,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01888 Ps_contig_mira-and-tgicl-ass_franssen_17160
SEQUENCE=CTACTTGTTTCATTCAGACAATGAAACAACATTGATTAAAACAAAAGTGATAAATTAAAGAAACAGTTGCAACAAACAAACATGAAAATGTTGTTTTTCCTCTATTTTCTGCTAAACAAGCTTCTCAATTTCCCTCTTTCCAATCTCTCAAGCAACTTTTTAGCTCCTGGAATCTCCATTTCAATAAGCTTCTTCAAATACCCTATTGCTCCATATGAAATCATCAACTTCTTACACTTCTTACATGAAGAAAGTGGTCCAAGACAACAAACTGCTATTTACTTTTTTTGCTAGTTATTCTGTGGACTAGGATCAAGCAACTGTACCTAAAG
TARGET=71,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01889 Ps_contig_mira-and-tgicl-ass_franssen_17162
SEQUENCE=TTTTGATCATACCTAATAAGAAAACATGCTCATAAAGAGAACTTGTCCTCTATGACCAATGCACAACTTACATGCAAACTTGAAGAATATAACCATACATACATATATCAAACAATGGGTAGAAATCCCCAAAATACAACTACTAATAAATGACTAAACTAGGACCCTTATTTAGACAACTAGAAAGACAACTTCTTTTATTAAGACTAGGAAGAAGGTACAGGGCATGAAGCCAAACTCCAAACGATGATGATTACAAGTACAAATGTTGCGCAAGCCTTGGACGAGGGTGGACCAAAAGAGGCTTTTCCCTTTGTAAAGTAAGCCCATATGCTTCATCCATGTTGAGTTTTTTCCGGCAATAACCCATTCTCCAACTCCCAATCGAATGCATGAACCAAAGTAGCAGTGAGAAGTTGAACCATCCTAAGTCCAAGACTCATACCAGCACAAATTCTACGTCCAGCACCAAACGGTATCACCTCAAAGTCAGTTCCCTTAATATCAACATCAAACTTCTCACCACCAGGGAGAAACCTTTCCGGCTTGAACTCCAATGGGTTGGACCATTCTTTAGGGTCACGTGATATGGCCCAAACATTAACCAACAGAGTTGCACCTTTAGGGATATGATAGTTGAAGATCTCACAACTCTCATTTGCGACACGTGGGAGCGAAAGAGGCGTAGATGGATGAAGTCGAAACGTTTCCTTCACCACAGCCTCTAAGTAAGGAAGATGAGCCAAGTCCATTTCATTAACCAACCTGTTTGCTCCAACAACAGTGTCCAACTCTTTTTGAATGGAAACCATAAGTCCGGGGTTTCTTATTAGTTCAGCAATAGCCCACTCTGTCGTGCTTGATGACGTGTCTGTTCCTGCTGCGAACATGTTCAAGAGTAAAGCTTTGATCTGGGTATCATTGAGTTTGTCTTCATCTTCATCAACTTTTTCTTTTAGTGACAGTAACTTGCTCAACAAGTCATTATGTCTTTCTTCTACTTTCTTTTGGTTTAAACC
TARGET=94,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1304
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=TCCTCTATGACCAATGCACAAC
PRIMER_RIGHT_SEQUENCE=CTTCATGCCCTGTACCTTCTTC
PRIMER_LEFT=45,22
PRIMER_RIGHT=231,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.1304
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=GTCCTCTATGACCAATGCACAA
PRIMER_RIGHT_1_SEQUENCE=CTTCATGCCCTGTACCTTCTTC
PRIMER_LEFT_1=44,22
PRIMER_RIGHT_1=231,22
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=188
PRIMER_PAIR_PENALTY_2=0.1304
PRIMER_LEFT_2_PENALTY=0.000037
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=TTGTCCTCTATGACCAATGCAC
PRIMER_RIGHT_2_SEQUENCE=CTTCATGCCCTGTACCTTCTTC
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=231,22
PRIMER_LEFT_2_TM=60.000
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.2276
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.227550
PRIMER_LEFT_3_SEQUENCE=TCCTCTATGACCAATGCACAAC
PRIMER_RIGHT_3_SEQUENCE=CTTGCGCAACATTTGTACTTGT
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=276,22
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.228
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=232
PRIMER_PAIR_PENALTY_4=0.2276
PRIMER_LEFT_4_PENALTY=0.000037
PRIMER_RIGHT_4_PENALTY=0.227550
PRIMER_LEFT_4_SEQUENCE=TTGTCCTCTATGACCAATGCAC
PRIMER_RIGHT_4_SEQUENCE=CTTGCGCAACATTTGTACTTGT
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=276,22
PRIMER_LEFT_4_TM=60.000
PRIMER_RIGHT_4_TM=60.228
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_01890 Ps_contig_mira-and-tgicl-ass_franssen_17169
SEQUENCE=GATTCGGCTGAAGTAACACAAAAACCACTGAAGTTTTGTTTGCTCCGCACTTGGCGATTCAATTTTGTTAATCTTCCCAATGAGAGGAAGCATCGGAGTAAGGCTTCAAAATTCCAACATTTCCAATGCCACAAGAAAAACCCTAGTTTCATTTTCTACATCTTCCGGTTTCGGCGGCGGCGGTGATGGCCGTGGCGGTGGCCGTGGTCGAGGAGGCTCTTTCTCTGGCCCGCCACAGTTTAATTTCAACGAAAAAGCTCCGGGTAACCCTAACCCTAACTCCAGCGAATCGAAATCTGACACAACAGATTCACCAATTCCTCCTGGTTCTGGCCGTGGCCATGGTCGTGG
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2590
PRIMER_LEFT_PENALTY=0.035021
PRIMER_RIGHT_PENALTY=0.224014
PRIMER_LEFT_SEQUENCE=TGCCACAAGAAAAACCCTAGTT
PRIMER_RIGHT_SEQUENCE=GATTTCGATTCGCTGGAGTTAG
PRIMER_LEFT=126,22
PRIMER_RIGHT=296,22
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=60.224
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=0.2590
PRIMER_LEFT_1_PENALTY=0.035021
PRIMER_RIGHT_1_PENALTY=0.224014
PRIMER_LEFT_1_SEQUENCE=TGCCACAAGAAAAACCCTAGTT
PRIMER_RIGHT_1_SEQUENCE=AGATTTCGATTCGCTGGAGTTA
PRIMER_LEFT_1=126,22
PRIMER_RIGHT_1=297,22
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=60.224
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.3964
PRIMER_LEFT_2_PENALTY=0.035021
PRIMER_RIGHT_2_PENALTY=0.361394
PRIMER_LEFT_2_SEQUENCE=TGCCACAAGAAAAACCCTAGTT
PRIMER_RIGHT_2_SEQUENCE=CAGGAGGAATTGGTGAATCTGT
PRIMER_LEFT_2=126,22
PRIMER_RIGHT_2=325,22
PRIMER_LEFT_2_TM=60.035
PRIMER_RIGHT_2_TM=60.361
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=0.4525
PRIMER_LEFT_3_PENALTY=0.228464
PRIMER_RIGHT_3_PENALTY=0.224014
PRIMER_LEFT_3_SEQUENCE=ATGAGAGGAAGCATCGGAGTAA
PRIMER_RIGHT_3_SEQUENCE=GATTTCGATTCGCTGGAGTTAG
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=60.228
PRIMER_RIGHT_3_TM=60.224
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.4525
PRIMER_LEFT_4_PENALTY=0.228464
PRIMER_RIGHT_4_PENALTY=0.224014
PRIMER_LEFT_4_SEQUENCE=AATGAGAGGAAGCATCGGAGTA
PRIMER_RIGHT_4_SEQUENCE=GATTTCGATTCGCTGGAGTTAG
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=296,22
PRIMER_LEFT_4_TM=60.228
PRIMER_RIGHT_4_TM=60.224
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_01891 Ps_contig_mira-and-tgicl-ass_franssen_17195
SEQUENCE=GGAATCGGATGACGGAGCGGGATTTTATGAGAGAGGCCACGGGACCTGCGGTTTGGAGTTGGGTTTCGCAACGGTGGTGAATGGCTCTGTTACAGTGTTTCGAAAATGATAATACACGATTCATGGTTATTCCCTTTTTTTTTTGGATTTTGAGAAGATGAGGACAACGAGAAGAAGAAGATGTTGGTGTTTTGGGTTTAGGAATTTACGGAAGAGTTGAAGACGACGTGGTTAGTTGGTAGATTGACATGTTTGGTTAGAGATCGATAGGTGTTCGATGAAAGTTCTGAGAGAAATCCACTCATCTACTCTTCGCGACACTTGTGTTGTGCGTGCTGACCAAGCTTGTTCCTTTTTCTTTTTCTTATGTTATSTTTTGTTTTCGTGTTTCATTTTATTCCCCGAC
TARGET=170,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01892 Ps_contig_mira-and-tgicl-ass_franssen_17196
SEQUENCE=GTTTGGAGTTGGGTTTCGCAACGTTGGTGAATTGCTCTGTTACAGTGTTTGGATAATGATAATACACGATTCATGGTTATTTCCCTTTTTTTTTTGGTATTTTTGAGAAGATGAGGACAACGAGAAGAAGAAGAAGAAGATGTTGGTGTTTTGGGTTTAGGAATTTACGGAAGAGTTGAAGACAACGTGGTTAGTTGGTAGATTGACATGTTTGGTTAGAGATCGATAGGTGTTCGATGAAAGTTCTGAGAGAAATCCACTCATCTACTCTTCGCGACA
TARGET=123,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1154
PRIMER_LEFT_PENALTY=0.048570
PRIMER_RIGHT_PENALTY=0.066849
PRIMER_LEFT_SEQUENCE=ACACGATTCATGGTTATTTCCC
PRIMER_RIGHT_SEQUENCE=CCAACTAACCACGTTGTCTTCA
PRIMER_LEFT=63,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.951
PRIMER_RIGHT_TM=60.067
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=0.1419
PRIMER_LEFT_1_PENALTY=0.075027
PRIMER_RIGHT_1_PENALTY=0.066849
PRIMER_LEFT_1_SEQUENCE=CACGATTCATGGTTATTTCCCT
PRIMER_RIGHT_1_SEQUENCE=CCAACTAACCACGTTGTCTTCA
PRIMER_LEFT_1=64,22
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=60.067
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=0.1708
PRIMER_LEFT_2_PENALTY=0.048570
PRIMER_RIGHT_2_PENALTY=0.122257
PRIMER_LEFT_2_SEQUENCE=ACACGATTCATGGTTATTTCCC
PRIMER_RIGHT_2_SEQUENCE=TTCCTAAACCCAAAACACCAAC
PRIMER_LEFT_2=63,22
PRIMER_RIGHT_2=162,22
PRIMER_LEFT_2_TM=59.951
PRIMER_RIGHT_2_TM=60.122
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=100
PRIMER_PAIR_PENALTY_3=0.2579
PRIMER_LEFT_3_PENALTY=0.048570
PRIMER_RIGHT_3_PENALTY=0.209343
PRIMER_LEFT_3_SEQUENCE=ACACGATTCATGGTTATTTCCC
PRIMER_RIGHT_3_SEQUENCE=ACGTTGTCTTCAACTCTTCCGT
PRIMER_LEFT_3=63,22
PRIMER_RIGHT_3=187,22
PRIMER_LEFT_3_TM=59.951
PRIMER_RIGHT_3_TM=60.209
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=0.2844
PRIMER_LEFT_4_PENALTY=0.075027
PRIMER_RIGHT_4_PENALTY=0.209343
PRIMER_LEFT_4_SEQUENCE=CACGATTCATGGTTATTTCCCT
PRIMER_RIGHT_4_SEQUENCE=ACGTTGTCTTCAACTCTTCCGT
PRIMER_LEFT_4=64,22
PRIMER_RIGHT_4=187,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=60.209
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_01893 Ps_contig_mira-and-tgicl-ass_franssen_17199
SEQUENCE=GGGGGTTTCACAACACCATCAGCAAAACGSGCGCACAGACACACTCTCTCTCTTCGATGCTTCGCCGCAATGTTCCGTTCATCACCTCAAACCAGCCGCGCATCACAATCATCTCCAACCCTCCGAA
TARGET=44,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01894 Ps_contig_mira-and-tgicl-ass_franssen_17200
SEQUENCE=ATGGTTGTTGGTTTCTTGCGAAGCTCCATTACTCTTGTCGAGTTCGAGGACTCGGTGGTTAGGTTTTTTCTTTGGTCTTCTTCCGCCGCTTGATTTTTGGCGGAGGGTTGAGATGATTGTGATGCGCGGCTGGTTTGAGGTGATGAATGGAACATTGCGGCGAAGCATCGAAGAGAGAGAGAGTGTGTCTGTGCGCGCGTTTTGCTGWTGGTGTTGTCGAAACCCCMYTTATTAAAATGGATTC
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01895 Ps_contig_mira-and-tgicl-ass_franssen_17218
SEQUENCE=TTAGCTCATGAATTTAAAAAATTATTTAATTTAGAGCAACAAAAAACACTAATAATATCGAAATTAAGATTACAATTACAAATTACAGACAGAACTTTCTATTTTGTTTTTTACTTTTTTTTTACTCTCTTTATTTCTCCCTGCCTCGCTCGCTCGCTCACTCACTGGGTCTACTTTTCAGTCATCAACATACAAAGTATTTAGTTTCAGCCAATCGTCGG
TARGET=145,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.9405
PRIMER_LEFT_PENALTY=3.451169
PRIMER_RIGHT_PENALTY=0.489375
PRIMER_LEFT_SEQUENCE=TTTACTCTCTTTATTTCTCCCTGCC
PRIMER_RIGHT_SEQUENCE=CGACGATTGGCTGAAACTAAAT
PRIMER_LEFT=120,25
PRIMER_RIGHT=219,22
PRIMER_LEFT_TM=60.451
PRIMER_RIGHT_TM=60.489
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=5.8133
PRIMER_LEFT_1_PENALTY=2.656431
PRIMER_RIGHT_1_PENALTY=3.156894
PRIMER_LEFT_1_SEQUENCE=TTACTCTCTTTATTTCTCCCTGCC
PRIMER_RIGHT_1_SEQUENCE=CCGACGATTGGCTGAAACTA
PRIMER_LEFT_1=121,24
PRIMER_RIGHT_1=220,20
PRIMER_LEFT_1_TM=59.344
PRIMER_RIGHT_1_TM=61.157
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=6.0224
PRIMER_LEFT_2_PENALTY=2.656431
PRIMER_RIGHT_2_PENALTY=3.365977
PRIMER_LEFT_2_SEQUENCE=TTACTCTCTTTATTTCTCCCTGCC
PRIMER_RIGHT_2_SEQUENCE=CCGACGATTGGCTGAAACTAA
PRIMER_LEFT_2=121,24
PRIMER_RIGHT_2=220,21
PRIMER_LEFT_2_TM=59.344
PRIMER_RIGHT_2_TM=62.366
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=100
PRIMER_PAIR_PENALTY_3=6.5340
PRIMER_LEFT_3_PENALTY=3.451169
PRIMER_RIGHT_3_PENALTY=3.082864
PRIMER_LEFT_3_SEQUENCE=TTTACTCTCTTTATTTCTCCCTGCC
PRIMER_RIGHT_3_SEQUENCE=CGACGATTGGCTGAAACTAA
PRIMER_LEFT_3=120,25
PRIMER_RIGHT_3=219,20
PRIMER_LEFT_3_TM=60.451
PRIMER_RIGHT_3_TM=58.917
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=6.6081
PRIMER_LEFT_4_PENALTY=3.451169
PRIMER_RIGHT_4_PENALTY=3.156894
PRIMER_LEFT_4_SEQUENCE=TTTACTCTCTTTATTTCTCCCTGCC
PRIMER_RIGHT_4_SEQUENCE=CCGACGATTGGCTGAAACTA
PRIMER_LEFT_4=120,25
PRIMER_RIGHT_4=220,20
PRIMER_LEFT_4_TM=60.451
PRIMER_RIGHT_4_TM=61.157
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_01896 Ps_contig_mira-and-tgicl-ass_franssen_17222
SEQUENCE=AGWBATTTTACAAAACAAATCGCTAATTTCGTAATATCAACTTCATCTCTCTCTCACACCACGCTGCGCTTCTCAACTGAGGCTTTTTAATTTAAGGGACAGAAAGAGGGTATGGTAATTTGTAGAATTATTGAATTGTAATAATCAACGTGGCAACAGACTGATTGTGAAAGGAGTTTTGTTGGGCGATCTTACTGACACTACTGAGTTTTATTTGGAGTTTTGTTGTGTTTAATAGTTCAGGACGTAATCTGAAGATCTGTGATG
TARGET=46,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01897 Ps_contig_mira-and-tgicl-ass_franssen_17240
SEQUENCE=CTCAATTAAGCCCTTATAGCATAAGCACTTAAATGCCAAGTGCTTATGGACAAAATGTTGTTCTATGTAGGGGCAGAGTTATCAGTCCCAGTTAGCGGGGCAGAGCGGCGGACTCCAAAAATGAGATAGAAGGATAACCACAATTTGACTATTTTTTAGACTAATTATATGAATAATCTATAAAACATATATTTAATGTAAAACCAAATAAATGAAAGAGGAGGAAGAGGAATAGAGTTATTAAATGACTTCACTTAAAGAAAAAAGAACAATATGAATTAAAAAAAACAAGAGGACAACACGAAGAAAGAAGAGAAAAGAAAGAAGGAGAAAAGAGACAAAAAAAACAAGAGTAAACAAAGAAAAGAAAATAAAGAAAAAGAGAAAAGAAAGAAAGAAAAAGAAAATAAAGAAGAAAAGAAGATCAGAGAGGTAAAAACCAATTTGAGAAGAGGGGATTGCAGACGGCGACACTAAGAAACAA
TARGET=386,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1571
PRIMER_LEFT_PENALTY=0.974437
PRIMER_RIGHT_PENALTY=0.182645
PRIMER_LEFT_SEQUENCE=CAAGAGGACAACACGAAGAAAG
PRIMER_RIGHT_SEQUENCE=ATCCCCTCTTCTCAAATTGGTT
PRIMER_LEFT=288,22
PRIMER_RIGHT=458,22
PRIMER_LEFT_TM=59.026
PRIMER_RIGHT_TM=60.183
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=1.1571
PRIMER_LEFT_1_PENALTY=0.974437
PRIMER_RIGHT_1_PENALTY=0.182645
PRIMER_LEFT_1_SEQUENCE=CAAGAGGACAACACGAAGAAAG
PRIMER_RIGHT_1_SEQUENCE=AATCCCCTCTTCTCAAATTGGT
PRIMER_LEFT_1=288,22
PRIMER_RIGHT_1=459,22
PRIMER_LEFT_1_TM=59.026
PRIMER_RIGHT_1_TM=60.183
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=1.2957
PRIMER_LEFT_2_PENALTY=1.113098
PRIMER_RIGHT_2_PENALTY=0.182645
PRIMER_LEFT_2_SEQUENCE=ACAAGAGGACAACACGAAGAAA
PRIMER_RIGHT_2_SEQUENCE=ATCCCCTCTTCTCAAATTGGTT
PRIMER_LEFT_2=287,22
PRIMER_RIGHT_2=458,22
PRIMER_LEFT_2_TM=58.887
PRIMER_RIGHT_2_TM=60.183
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=1.2957
PRIMER_LEFT_3_PENALTY=1.113098
PRIMER_RIGHT_3_PENALTY=0.182645
PRIMER_LEFT_3_SEQUENCE=AACAAGAGGACAACACGAAGAA
PRIMER_RIGHT_3_SEQUENCE=ATCCCCTCTTCTCAAATTGGTT
PRIMER_LEFT_3=286,22
PRIMER_RIGHT_3=458,22
PRIMER_LEFT_3_TM=58.887
PRIMER_RIGHT_3_TM=60.183
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=173
PRIMER_PAIR_PENALTY_4=1.2957
PRIMER_LEFT_4_PENALTY=1.113098
PRIMER_RIGHT_4_PENALTY=0.182645
PRIMER_LEFT_4_SEQUENCE=ACAAGAGGACAACACGAAGAAA
PRIMER_RIGHT_4_SEQUENCE=AATCCCCTCTTCTCAAATTGGT
PRIMER_LEFT_4=287,22
PRIMER_RIGHT_4=459,22
PRIMER_LEFT_4_TM=58.887
PRIMER_RIGHT_4_TM=60.183
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_01898 Ps_contig_mira-and-tgicl-ass_franssen_17242
SEQUENCE=CCATCAAAAACAAGATGAAAATGGATGGTGTTGAATCTGGTTGTGGTGATGGTGAGGATGAGGATGATTGTTCTCCATTGCAGGTGAACCAGAAGCAGGAGAAGAAGAAGTAGAAGCAGCACCTCCTCCTCCTCCTCCCATGAAAAAAGGTAGATTGTTGGATCCGTCTAAAGATGAAACTGAAAACCTCTGAAGATGAGACAAAAGAGACGGTGAATTGGACTGAAGGGTCCCCCTATTGTAACCAGCAAGGCCCGATGAGATAGTAAAGTTGAGATTATAATCGCTTGGTTGAGTCGAAGAAGAAGTAACCACATTTTGCATCAT
TARGET=122,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4985
PRIMER_LEFT_PENALTY=0.067436
PRIMER_RIGHT_PENALTY=0.431057
PRIMER_LEFT_SEQUENCE=TGAGGATGATTGTTCTCCATTG
PRIMER_RIGHT_SEQUENCE=TCATCTTTAGACGGATCCAACA
PRIMER_LEFT=58,22
PRIMER_RIGHT=176,22
PRIMER_LEFT_TM=59.933
PRIMER_RIGHT_TM=59.569
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.5184
PRIMER_LEFT_1_PENALTY=0.067436
PRIMER_RIGHT_1_PENALTY=0.450944
PRIMER_LEFT_1_SEQUENCE=TGAGGATGATTGTTCTCCATTG
PRIMER_RIGHT_1_SEQUENCE=TTTACTATCTCATCGGGCCTTG
PRIMER_LEFT_1=58,22
PRIMER_RIGHT_1=269,22
PRIMER_LEFT_1_TM=59.933
PRIMER_RIGHT_1_TM=60.451
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.5931
PRIMER_LEFT_2_PENALTY=0.162074
PRIMER_RIGHT_2_PENALTY=0.431057
PRIMER_LEFT_2_SEQUENCE=GATGGTGAGGATGAGGATGATT
PRIMER_RIGHT_2_SEQUENCE=TCATCTTTAGACGGATCCAACA
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=176,22
PRIMER_LEFT_2_TM=60.162
PRIMER_RIGHT_2_TM=59.569
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=130
PRIMER_PAIR_PENALTY_3=0.5957
PRIMER_LEFT_3_PENALTY=0.067436
PRIMER_RIGHT_3_PENALTY=0.528219
PRIMER_LEFT_3_SEQUENCE=TGAGGATGATTGTTCTCCATTG
PRIMER_RIGHT_3_SEQUENCE=CAGTCCAATTCACCGTCTCTTT
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=224,22
PRIMER_LEFT_3_TM=59.933
PRIMER_RIGHT_3_TM=60.528
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=167
PRIMER_PAIR_PENALTY_4=0.6130
PRIMER_LEFT_4_PENALTY=0.162074
PRIMER_RIGHT_4_PENALTY=0.450944
PRIMER_LEFT_4_SEQUENCE=GATGGTGAGGATGAGGATGATT
PRIMER_RIGHT_4_SEQUENCE=TTTACTATCTCATCGGGCCTTG
PRIMER_LEFT_4=47,22
PRIMER_RIGHT_4=269,22
PRIMER_LEFT_4_TM=60.162
PRIMER_RIGHT_4_TM=60.451
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_01899 Ps_contig_mira-and-tgicl-ass_franssen_17259
SEQUENCE=CTTCTTCTTCTTCTCCAAGGACGTCGTCATTATCAGTGTTATCATCGTGTTGTTGCTGAAGAAGTTGAAGTTGAATTTGAGCTTGTTGTTGTTGTTTAATGATCCAGGCCTTTTCCCTCTCTTCGAAAGCGATTCTATCCCTTTCGATTCTCTCGGCCTCTTGCTGCTCCAACAGCATCCTCTTCTTCTGCTCCTCGGGATCGTTGAGACGCGCTTCCTCCTCCCAACCGTTCCATCCTCCGCAATCCTTCCTTCCTCTCTCCTTCCTYTCTAYTTCTATTTTCTTA
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01900 Ps_contig_mira-and-tgicl-ass_franssen_17259
SEQUENCE=CTTCTTCTTCTTCTCCAAGGACGTCGTCATTATCAGTGTTATCATCGTGTTGTTGCTGAAGAAGTTGAAGTTGAATTTGAGCTTGTTGTTGTTGTTTAATGATCCAGGCCTTTTCCCTCTCTTCGAAAGCGATTCTATCCCTTTCGATTCTCTCGGCCTCTTGCTGCTCCAACAGCATCCTCTTCTTCTGCTCCTCGGGATCGTTGAGACGCGCTTCCTCCTCCCAACCGTTCCATCCTCCGCAATCCTTCCTTCCTCTCTCCTTCCTYTCTAYTTCTATTTTCTTA
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01901 Ps_contig_mira-and-tgicl-ass_franssen_17259
SEQUENCE=CTTCTTCTTCTTCTCCAAGGACGTCGTCATTATCAGTGTTATCATCGTGTTGTTGCTGAAGAAGTTGAAGTTGAATTTGAGCTTGTTGTTGTTGTTTAATGATCCAGGCCTTTTCCCTCTCTTCGAAAGCGATTCTATCCCTTTCGATTCTCTCGGCCTCTTGCTGCTCCAACAGCATCCTCTTCTTCTGCTCCTCGGGATCGTTGAGACGCGCTTCCTCCTCCCAACCGTTCCATCCTCCGCAATCCTTCCTTCCTCTCTCCTTCCTYTCTAYTTCTATTTTCTTA
TARGET=246,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01902 Ps_contig_mira-and-tgicl-ass_franssen_17260
SEQUENCE=GTCGGGCACTACCCTCACACAGCTCCACTCAACCATTACTTTCCTTCAAACGAAAACTTTCTCCTTCTGTTTCCTCCTTCACCAGCGCACCTACCGCCACAAATCTTTTTCTCACTTCCGACAACAACCGTCCTCCTTCATAACCACCGCCGGCGGACACCGCGCACTGACACACACCGCCAACGACGAACTTCTTCTTCTTCATCCCACCGGCGCACCCTCAACTACCGGCCACCCAACACAACAATTACCTTCCGTCGCCGACATCAACACCACCGTGATTCACCTTCGCCATAAACAATCTGGTAAATCCCTTCCAGCGGAGCAATAGGGGGTCACCGAAATCCACTAAAAGGTCACCGGCGGCGGTGTCGCACAACGACCTGTTCCAAAACGTTCTCCGTTTCCATGAGGTTTACATGATATATAGGGTGTGCTTTAAGACCATTTTACTAATGCAAAAGCTTGGAAGGTTCAAAAGCAAGACTTGCAACTTGATTCATATTTGTTGAGATGTGACAACATCAAATGGTACATGGCGGTAGTTTTCTAAATCATTTTCTTTATTATATGGTGATCAGTTGCATGGAATTTTGATTTTAAGTAGAATTTGTTGTGCTTCTTTCTGGATTTTGTTTTGTGTGGATAGTGTATATGATTGACATTGCTGAATATAGTTTAGAAATTGGGTTTCTATTGTATTGGTAGGGCTAAGTATATTAAAAATAAACTCATCTTTTTTTTAANCGTATGGATGCTAATTTGTTGTTATAATGTC
TARGET=191,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4018
PRIMER_LEFT_PENALTY=0.273230
PRIMER_RIGHT_PENALTY=0.128591
PRIMER_LEFT_SEQUENCE=CTTCTGTTTCCTCCTTCACCAG
PRIMER_RIGHT_SEQUENCE=TGACCTTTTAGTGGATTTCGGT
PRIMER_LEFT=63,22
PRIMER_RIGHT=358,22
PRIMER_LEFT_TM=60.273
PRIMER_RIGHT_TM=59.871
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=0.4018
PRIMER_LEFT_1_PENALTY=0.273230
PRIMER_RIGHT_1_PENALTY=0.128591
PRIMER_LEFT_1_SEQUENCE=CTTCTGTTTCCTCCTTCACCAG
PRIMER_RIGHT_1_SEQUENCE=GTGACCTTTTAGTGGATTTCGG
PRIMER_LEFT_1=63,22
PRIMER_RIGHT_1=359,22
PRIMER_LEFT_1_TM=60.273
PRIMER_RIGHT_1_TM=59.871
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.4018
PRIMER_LEFT_2_PENALTY=0.273230
PRIMER_RIGHT_2_PENALTY=0.128591
PRIMER_LEFT_2_SEQUENCE=CTTCTGTTTCCTCCTTCACCAG
PRIMER_RIGHT_2_SEQUENCE=GACCTTTTAGTGGATTTCGGTG
PRIMER_LEFT_2=63,22
PRIMER_RIGHT_2=357,22
PRIMER_LEFT_2_TM=60.273
PRIMER_RIGHT_2_TM=59.871
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.4018
PRIMER_LEFT_3_PENALTY=0.273230
PRIMER_RIGHT_3_PENALTY=0.128591
PRIMER_LEFT_3_SEQUENCE=CTTCTGTTTCCTCCTTCACCAG
PRIMER_RIGHT_3_SEQUENCE=GGTGACCTTTTAGTGGATTTCG
PRIMER_LEFT_3=63,22
PRIMER_RIGHT_3=360,22
PRIMER_LEFT_3_TM=60.273
PRIMER_RIGHT_3_TM=59.871
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.4018
PRIMER_LEFT_4_PENALTY=0.273230
PRIMER_RIGHT_4_PENALTY=0.128591
PRIMER_LEFT_4_SEQUENCE=CTTCTGTTTCCTCCTTCACCAG
PRIMER_RIGHT_4_SEQUENCE=CGGTGACCTTTTAGTGGATTTC
PRIMER_LEFT_4=63,22
PRIMER_RIGHT_4=361,22
PRIMER_LEFT_4_TM=60.273
PRIMER_RIGHT_4_TM=59.871
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_01903 Ps_contig_mira-and-tgicl-ass_franssen_17263
SEQUENCE=CTTTCTCCCCCCTTTTTTATTTTATTTTAAATTTTGGGGGGTTTTTTTCGTTGGAATTGAAAAAAAATACGATAATTGTGTATTGCAGATCTTAAAGATTGGATTTTGAGAAGGTTTTGAACCTTTCTCGTCACTGCAAGTGCAACG
TARGET=15,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01904 Ps_contig_mira-and-tgicl-ass_franssen_17286
SEQUENCE=GAGGTSACTCTCATGATCTCATCCTACCACACACACACTGAGTGAACCCTGTAGATAGATGATCTTTCTTATCTGCATCTCCTAAACCCTTTCTTATTTTTATTCCTATTTCTATTTCTCCGATCATCATCATCGTCGTTAATTTGTTACCATGAGTGGAGTCG
TARGET=28,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01905 Ps_contig_mira-and-tgicl-ass_franssen_17286
SEQUENCE=GAGGTSACTCTCATGATCTCATCCTACCACACACACACTGAGTGAACCCTGTAGATAGATGATCTTTCTTATCTGCATCTCCTAAACCCTTTCTTATTTTTATTCCTATTTCTATTTCTCCGATCATCATCATCGTCGTTAATTTGTTACCATGAGTGGAGTCG
TARGET=123,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01906 Ps_contig_mira-and-tgicl-ass_franssen_17287
SEQUENCE=GATCCTACCKACACACACACTGAGTGAACCCTGTAGATAGATGATCTTTCTTATCTGCATCTCAAACCCTTTCTATTTTTTATTCCATTTCATTTCTCCGATCATCATCATCGTCGTTAATTTGTTACCATGAGTGGAGCTGTTGTTGTTGCTATTGCCGCTGCTATTCGGTTAATTTACTTCAAGGAGT
TARGET=11,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01907 Ps_contig_mira-and-tgicl-ass_franssen_17287
SEQUENCE=GATCCTACCKACACACACACTGAGTGAACCCTGTAGATAGATGATCTTTCTTATCTGCATCTCAAACCCTTTCTATTTTTTATTCCATTTCATTTCTCCGATCATCATCATCGTCGTTAATTTGTTACCATGAGTGGAGCTGTTGTTGTTGCTATTGCCGCTGCTATTCGGTTAATTTACTTCAAGGAGT
TARGET=101,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01908 Ps_contig_mira-and-tgicl-ass_franssen_17290
SEQUENCE=AACTAAGTTAGTTAATGAATGGGAAACTCAGTTAGTTAATTGGTTAACCTGGTCAATGTTTCTAGTTTAGTTTAGTTTAAGGTTGATTTTGRTTCTATGTGATGCAGTTAATTGTTATAATGGATTAATCATTTTGTTCTGRWAATTGTTATCACACTTAATTAAAAAAAGTT
TARGET=63,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01909 Ps_contig_mira-and-tgicl-ass_franssen_17291
SEQUENCE=ACCTTTAGAACAAAATGATTAATCCATTATAACAATTAACTGCATCACATAGAATCAAAATCACCTTAACTAACTAACTGAACATTGACCAGGTTAACCAATTAACTAACTGAGTTTCCCATTCATTAACTAACTTAGTTATCAGTTAACTCAGTGAGTCAACCTCAACTAACTCCAACCCTTCTCTAACAGACTCCAATCCTAACTCCAAACCTATCTAATTCCCTAACCTCCTCTCTATTTAAACAACTCCACTATCATCTTTGAGGGCTTGAACACTTTGCTCAGAAATTCACTTATTCACCTTCTTCCCCATTCGTGCACATTCTCACTGCAACCATTTGCAGAAACCACAGAACATCACACGCCACGAAAATGAGAATAATTCATTCCCAACGAACCACAACAAGCTGGGAAGGAGAACGACGTCGCAGGATCGTAGAACACGCTCGCCAAATTTGGAGGGAGAAAACGATGCCTCAGACGCCGCGATTACCGTCGTGGCTCTTCTTCGTGGAGGCGGTGTCTCGTTTGGATTGGAAGTGACGACGTTAAGTTTGGATGAGCGACAGTGAAGACGCAGCGTTGTTGATGCTCTAGAGCTCCAAGATTGATGCAGAAGCAAAGAGAAATCGCGTTGTGGATAAGCTTTTCAAATAAGCGCTTTTAGTGAACAACCATTCGCGTTTGGATTCCATMTGAATCTTCATCCCTGTC
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01910 Ps_contig_mira-and-tgicl-ass_franssen_17296
SEQUENCE=GATCCTTTGGTTTTCAGTCTCTCACAGACACCACAGATTCTTCTTCTTCTTGACATACCCATTATCCGTGAAACAGGGGAAGACCACCGCGCACCGCCACCCAAACTCCGTTCTCCGACCTCCCGCTGTTCGTGCTTTTCGCGTTCCTATTGTTTTGAGAGCACTGATTCTCAGCGAAAGCTCCTCGTTTAGCGTGACTTTTGCTTCCGATCTGCTCAAAATCTACACAAATCTTCGACCTATTCTTCGAGGAAGAAGAAGAAGAAGATCGACCTTAACAGGAGGAGATAAAAGG
TARGET=38,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5624
PRIMER_LEFT_PENALTY=0.510368
PRIMER_RIGHT_PENALTY=0.052069
PRIMER_LEFT_SEQUENCE=TCAGTCTCTCACAGACACCACA
PRIMER_RIGHT_SEQUENCE=AAAAGTCACGCTAAACGAGGAG
PRIMER_LEFT=13,22
PRIMER_RIGHT=201,22
PRIMER_LEFT_TM=59.490
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.6599
PRIMER_LEFT_1_PENALTY=0.510368
PRIMER_RIGHT_1_PENALTY=0.149516
PRIMER_LEFT_1_SEQUENCE=TCAGTCTCTCACAGACACCACA
PRIMER_RIGHT_1_SEQUENCE=CTTCCTCGAAGAATAGGTCGAA
PRIMER_LEFT_1=13,22
PRIMER_RIGHT_1=254,22
PRIMER_LEFT_1_TM=59.490
PRIMER_RIGHT_1_TM=59.850
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.7470
PRIMER_LEFT_2_PENALTY=0.510368
PRIMER_RIGHT_2_PENALTY=0.236656
PRIMER_LEFT_2_SEQUENCE=TCAGTCTCTCACAGACACCACA
PRIMER_RIGHT_2_SEQUENCE=GCTGAGAATCAGTGCTCTCAAA
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=174,22
PRIMER_LEFT_2_TM=59.490
PRIMER_RIGHT_2_TM=59.763
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=0.7742
PRIMER_LEFT_3_PENALTY=0.510368
PRIMER_RIGHT_3_PENALTY=0.263824
PRIMER_LEFT_3_SEQUENCE=TCAGTCTCTCACAGACACCACA
PRIMER_RIGHT_3_SEQUENCE=GCTCTCAAAACAATAGGAACGC
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=161,22
PRIMER_LEFT_3_TM=59.490
PRIMER_RIGHT_3_TM=60.264
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.8062
PRIMER_LEFT_4_PENALTY=0.510368
PRIMER_RIGHT_4_PENALTY=0.295843
PRIMER_LEFT_4_SEQUENCE=TCAGTCTCTCACAGACACCACA
PRIMER_RIGHT_4_SEQUENCE=GGAAGCAAAAGTCACGCTAAAC
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=207,22
PRIMER_LEFT_4_TM=59.490
PRIMER_RIGHT_4_TM=60.296
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_01911 Ps_contig_mira-and-tgicl-ass_franssen_17296
SEQUENCE=GATCCTTTGGTTTTCAGTCTCTCACAGACACCACAGATTCTTCTTCTTCTTGACATACCCATTATCCGTGAAACAGGGGAAGACCACCGCGCACCGCCACCCAAACTCCGTTCTCCGACCTCCCGCTGTTCGTGCTTTTCGCGTTCCTATTGTTTTGAGAGCACTGATTCTCAGCGAAAGCTCCTCGTTTAGCGTGACTTTTGCTTCCGATCTGCTCAAAATCTACACAAATCTTCGACCTATTCTTCGAGGAAGAAGAAGAAGAAGATCGACCTTAACAGGAGGAGATAAAAGG
TARGET=252,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8139
PRIMER_LEFT_PENALTY=0.052069
PRIMER_RIGHT_PENALTY=1.761821
PRIMER_LEFT_SEQUENCE=CTCCTCGTTTAGCGTGACTTTT
PRIMER_RIGHT_SEQUENCE=ATCTCCTCCTGTTAAGGTCGAT
PRIMER_LEFT=180,22
PRIMER_RIGHT=288,22
PRIMER_LEFT_TM=59.948
PRIMER_RIGHT_TM=58.238
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=1.8361
PRIMER_LEFT_1_PENALTY=0.074231
PRIMER_RIGHT_1_PENALTY=1.761821
PRIMER_LEFT_1_SEQUENCE=TGACATACCCATTATCCGTGAA
PRIMER_RIGHT_1_SEQUENCE=ATCTCCTCCTGTTAAGGTCGAT
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=288,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=58.238
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=1.8361
PRIMER_LEFT_2_PENALTY=0.074231
PRIMER_RIGHT_2_PENALTY=1.761821
PRIMER_LEFT_2_SEQUENCE=TTGACATACCCATTATCCGTGA
PRIMER_RIGHT_2_SEQUENCE=ATCTCCTCCTGTTAAGGTCGAT
PRIMER_LEFT_2=49,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=58.238
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=1.9985
PRIMER_LEFT_3_PENALTY=0.236656
PRIMER_RIGHT_3_PENALTY=1.761821
PRIMER_LEFT_3_SEQUENCE=TTTGAGAGCACTGATTCTCAGC
PRIMER_RIGHT_3_SEQUENCE=ATCTCCTCCTGTTAAGGTCGAT
PRIMER_LEFT_3=153,22
PRIMER_RIGHT_3=288,22
PRIMER_LEFT_3_TM=59.763
PRIMER_RIGHT_3_TM=58.238
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=2.0256
PRIMER_LEFT_4_PENALTY=0.263824
PRIMER_RIGHT_4_PENALTY=1.761821
PRIMER_LEFT_4_SEQUENCE=GCGTTCCTATTGTTTTGAGAGC
PRIMER_RIGHT_4_SEQUENCE=ATCTCCTCCTGTTAAGGTCGAT
PRIMER_LEFT_4=140,22
PRIMER_RIGHT_4=288,22
PRIMER_LEFT_4_TM=60.264
PRIMER_RIGHT_4_TM=58.238
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_01912 Ps_contig_mira-and-tgicl-ass_franssen_17300
SEQUENCE=GTCGGGGACAAAAATAGTATTGTACGGAAACAGGAAACAAAGATAGGAACATGACCATGAAAAGAAAGGAAACAACCTCACCCTCACATCATTTTTATTATATCTGAATCTCAACTTGCACAAAATAATAAATTCTGTTTGGGAATCGTAAATAGTTCCCTTCCGATCTCAATAAGATAAACTGGTTCGGTTTGTTCTTGTAACTTCCGCTTTCTCTCTCTCTCTCTCTTCATAGTTCAATAAAGTAACCTWAATTTGAGAGTGAGTGCAGTTTAAGAGAGAGAGGAATAATAATAGTAT
TARGET=213,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01913 Ps_contig_mira-and-tgicl-ass_franssen_17300
SEQUENCE=GTCGGGGACAAAAATAGTATTGTACGGAAACAGGAAACAAAGATAGGAACATGACCATGAAAAGAAAGGAAACAACCTCACCCTCACATCATTTTTATTATATCTGAATCTCAACTTGCACAAAATAATAAATTCTGTTTGGGAATCGTAAATAGTTCCCTTCCGATCTCAATAAGATAAACTGGTTCGGTTTGTTCTTGTAACTTCCGCTTTCTCTCTCTCTCTCTCTTCATAGTTCAATAAAGTAACCTWAATTTGAGAGTGAGTGCAGTTTAAGAGAGAGAGGAATAATAATAGTAT
TARGET=276,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01914 Ps_contig_mira-and-tgicl-ass_franssen_17302
SEQUENCE=TACTACATTAAATTTAMCAACATTTTATTGTAGTATAATGGCTATGATGGTGTAAGACACACATTATAATAGGATGAGGCAACATAAGATTAATTATTTTATTTAGTTATAATTATATTGAGCTCGCCCTCCACAGTATTATACTATTTATTCGTCCTTACGTAGTTGGTATTCTATTCTATTCATC
TARGET=170,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01915 Ps_contig_mira-and-tgicl-ass_franssen_17311
SEQUENCE=GATTATKAACGGATTTAATCAAACATATTATTAYAACCACTTTTTCTCCATTTTTCAGAACCAAACCCTTAACACTCTGTCTCCGTGAATCTAAAATCTACCTTTGGGTCTGTCGGGTGTGTGAGGAACCGGTGAGCGAGCGAGCTAGCGCCGAAAAAATGGAAGATGCTGAAACGAAAGAAAGAATCGAAGAAACAGTTCTGAAGATCTTACAAGAATCAAACATGGATGAAGTCACTGAATCCAAGATTCGTAAGCAAGCCTCCAACGAACTCGACCTCAACCTATCTCAGCCTCCTTTCAAAGCCTTAGTCAAGCAAATAATCGAAGACTTTTACTCAAACAGAAGCAGCAACAGCAAGAGGAAGAGGAAGAAGAAAAGGAGGAAGAAGAAAAACAAAAGCTCAAACAAAGTAGGGAGTTTCCAGCAGAAACAACAATGTCT
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01916 Ps_contig_mira-and-tgicl-ass_franssen_17333
SEQUENCE=CCGTTGCATTAAGCCGGACATTCGGAACTTCTTCTCGACTTTACTTACCGTGCCTAAACATGCTGACTAGTGCGGATCCTCAGATTTTAATCTTTTTGTGAAGAAGTATGAAAATGTACAAAGCTGTGGTTAATAATATATTTGCATGCCATTCTTTGGTAACAACCTTAGAGCATGTCTGCTTCTGCAGGGTTAACAAACTCTTCAAAGTGTAGCTTCTGTCCAATTGCACAGAAGCACCTTGTATTTTTGTTATTTTTTCTTAACACATTTCATAGTGAAAGAATCATTTATTTATTTAGCAAGATAATTTGGCCTTATTTTAAGCTGGTGAATACTGGTTTTGGTCCGAAATTGTTGGTGAATACTGGTTTTGGTCTGAAATTGTTGATGTTAAAGTGAC
TARGET=289,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3326
PRIMER_LEFT_PENALTY=0.115769
PRIMER_RIGHT_PENALTY=0.216800
PRIMER_LEFT_SEQUENCE=ATGCCATTCTTTGGTAACAACC
PRIMER_RIGHT_SEQUENCE=TTTCGGACCAAAACCAGTATTC
PRIMER_LEFT=145,22
PRIMER_RIGHT=353,22
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=60.217
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.3530
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.237199
PRIMER_LEFT_1_SEQUENCE=ATGCCATTCTTTGGTAACAACC
PRIMER_RIGHT_1_SEQUENCE=CCAAAACCAGTATTCACCAACA
PRIMER_LEFT_1=145,22
PRIMER_RIGHT_1=376,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=59.763
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.3915
PRIMER_LEFT_2_PENALTY=0.174657
PRIMER_RIGHT_2_PENALTY=0.216800
PRIMER_LEFT_2_SEQUENCE=GCTTCTGCAGGGTTAACAAACT
PRIMER_RIGHT_2_SEQUENCE=TTTCGGACCAAAACCAGTATTC
PRIMER_LEFT_2=180,22
PRIMER_RIGHT_2=353,22
PRIMER_LEFT_2_TM=59.825
PRIMER_RIGHT_2_TM=60.217
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.3981
PRIMER_LEFT_3_PENALTY=0.115769
PRIMER_RIGHT_3_PENALTY=0.282356
PRIMER_LEFT_3_SEQUENCE=ATGCCATTCTTTGGTAACAACC
PRIMER_RIGHT_3_SEQUENCE=ACCAAAACCAGTATTCACCAGC
PRIMER_LEFT_3=145,22
PRIMER_RIGHT_3=347,22
PRIMER_LEFT_3_TM=60.116
PRIMER_RIGHT_3_TM=60.282
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.4041
PRIMER_LEFT_4_PENALTY=0.187326
PRIMER_RIGHT_4_PENALTY=0.216800
PRIMER_LEFT_4_SEQUENCE=AATTGCACAGAAGCACCTTGTA
PRIMER_RIGHT_4_SEQUENCE=TTTCGGACCAAAACCAGTATTC
PRIMER_LEFT_4=224,22
PRIMER_RIGHT_4=353,22
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=60.217
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_01917 Ps_contig_mira-and-tgicl-ass_franssen_17337
SEQUENCE=CGAAAATAGAATTAGTCTGACTAGTGGCTGTAAATAGGACAGGAAATAGAATACTAAAAGTAAATAAAATACACATATGGGCCAGTACATGACTCCATTTAACAATGTAGGAAGCAAGTCACGCTTTAAACGGGCAACAATCTCTTTACCGTTTTCACCTTGCACACCAGCATTCGAGGAGAAGAAAATAACAGTCATGGCAGGTCCATAAAGTGTCTGGCCCATGACCATTTTTTTCAATGTAGAGAAAAGATCCCTCTTTGGGAAAAGCTTTGACACAAAATTGAACCAGAAATGTAGTGTTGGTCCTAAAATGATCAATCCATATCCCGCCATGCGTAAAGTCCTTATAAAATCATAGGATTTTGATGGTTGCCACTCAATAGTCTGACGCTCAATAGTCTGGGAGGATAAATCAGCAGCGGTATAAATGAGTGCAGAAGTAACACTTTTGGTGAGAATAGGGTGTGACTTAATCATCCCCAAATACCAACCTACAAAACCAACTTTCGAAACAGAAGCTGTTGAAGAAGAAGACGAAGAAGAAGAACAAAATGAAGAGGAAAACAAAGACGGAAAATTTTCATATTGTTTGGGTTTCTTAACAAAATCCGGTAATCGAAAGTAAGGTTTAGAGTGAAGGTTGTTGCAGAGAAAAGAATGAACTCTGATTGGATTACAGGAATGGTTTATGAACATTGATTGTCTCTGCAACATGATGAAATGGGAAACCTTAGAGCGGTTCTTGAGAACAATACCGCTCATGTTTTTGGTTTCAGGGTGTTGGGGTTTTTAGATTCAGAGAAACATAAATATAAAAATGTGTCAGAGTTCCGGGTTCGTGGGAATGGGTGGTGCAAATAGCAAAAACTCATCCATGTCMYGAGTTTGCTATTGCGGCTTCTTCAACAACCTCACCGGTCATC
TARGET=539,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01918 Ps_contig_mira-and-tgicl-ass_franssen_17367
SEQUENCE=TGGTGCTGTGGTTGTTTCTTGTGAAGTTGTTTTGTTTGAACATTTGTTGTGGATGCTTGAGAGTGGTGGAAATGAGACACAATTGGGATCCATGGATGAACTTGTTGAGTTCTATAACTGTGGTGCATGTTGATTATGCTTTGTTGACATAGCTAGCTAGTAATAACAGTAAGTAACTGACTTCAATTGTTTTGTTTTCTTGTGGTGATTCAAAAATAGGTGAAGTGGTCAATTGAGATTGATTAAGTGTATTTAAGTGGTTTTATTTTATATATATATTATACTATTGCCAACG
TARGET=269,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01919 Ps_contig_mira-and-tgicl-ass_franssen_17372
SEQUENCE=AAGGTTCGTGTCTGTGACACGCATTCCTYYAATGAAGAAAATTTTGGATACTATTGTTGAAGAAGAGAATAATGATTTGGTGGTGACAAATGCTTTTCATCATCAACAACAACAACTTTGATGAACAACTTGTAGTTTGTAGTTCTTCATTGCGTATTTTTTGAGTATTCTTTTTTTGTTGAGAGAAGAATAAATGAGACATGGTTTGTTTGTGCTTTAGAACAAAGTTACCATGACTCATGAAATAAATAGGCAAAGAGAGAATTAACACCACTTGAGTTTTCACAATGTGATGTTTTTCTTGATGTCTTTTAGGTTTTAGTTTTTTCTATTTTGCCATAATGTTCTTTGGAGTTTTTCACCCAATATTTTAGCGTGATTGCTCCTTATCTCAAATAATAATTCTTTAGTTTTGTTTTATTTGGATCTAATGTTCATTGAAGTAAATCACTCCATTTTGGTAG
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01920 Ps_contig_mira-and-tgicl-ass_franssen_17373
SEQUENCE=CAACGCAGAGTACGCGGGGAAGGATATATACCCTACTTACTCTTCTTTTTCTTTATGTCTCATTTTTCTTCACGTGCTCACCACATGTTCATCCCCAAAACACTAGAGTTTTATGAAATGGCCAACAAGTTGAAGCAAATTGCTAGATTAATCACCACAGAGGTTGCTCCACCAAGGTTCGTGTCTGTGACACGCATTCCAATGAAGAAAATTTTGGATACTATTGTTGAAGAAGAGAATAATGATTTGGTGGTGACAAATGCTTTTCNTCATCAACAACAACAACTTTGAT
TARGET=274,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01921 Ps_contig_mira-and-tgicl-ass_franssen_17388
SEQUENCE=CCTTTTATTAGAATGGTATATATAGATAGATAGCAAATCTGGACTACATTAATCACATCCAAAACTTTGTATCCTAATTACACTACAAATTCATACATTCATACATATACACACAATAATCTATAGCCCTTTACCTTCACTTTCGGCCACCATTTGATCCAATTCTTCCCTGAAACAAATTGTTAAGCCGAGCTTCAATGTCCTCCATTCCAAACTTGATATACTTATCACCATTCTTAGCAAGCTCCGGAATAATTTGAAACCTTCCAATAAAGCTTCCATATGCAGGCAACAAGNGCTTTCAACTGANATCCTAATTCT
TARGET=22,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01922 Ps_contig_mira-and-tgicl-ass_franssen_17391
SEQUENCE=GGTATCAACGCAGAGTTATGGGCTTTGAAGGCAGCCACCATGATACTCAATCTTACTCCTGCGGACAATGGGGCTTCTCTCTCCGTTTGAATGAATGAATGTATATCAACTTCATTTGCAATATATTTTGTTTTTTTGTAACATAGAGACAGAACAAATGAGGCCAATCATCAAGTTCAGAATCATATGATATGACTGTATAATTCAAACTACTATCAATCTATCATTGACATTGTAAGATTCGGCGTAGTTTCGTATTTCYTATTTTACTTATACTTATGTTCG
TARGET=88,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01923 Ps_contig_mira-and-tgicl-ass_franssen_17394
SEQUENCE=GACGGSGGATCTCTTCACATATAAGTAGCTATCTGTTAATTCTAATTTGAATTATATCTTCTTCTTTTCCATCTCTTTCACATATAAGTAGCTCTCTGTTAGTTCTAATTTAAAACATATCTTCTTTTAATTAATTAATTATGTCAAAGGGAAAATATGCTGCTGATATTTCCTCCATACAAGAAGCATACACCAGAATTAAATCATATGTCATTAAAACTCCAATTATCTCGTCCACCTCTCTTGATGACATATCAAGAAGACAATTATACTTTAAGTGTGAAAATTTTCAAAAGAGTGGAGCTTTTAAGTTCAGAGGTGCCTGCAATGCAGTTTTCTCTCTCCCTGATGAAGCTATTTCTAAAGGTGTTATAACACACAGCAGTGGAAATCATGCTGCCGCATTGTCTCTGGCAGCAAAACTAAGAGGAGTCCCTGCATACATTGTTGTTCCAAACAATGCACCAACTTGTAAAGTTGAGAATATTAAGCGTTATGATGGTAGGGTTAACTTCTCTGAGACCAATATGCGGTCGAGGATAGAAGTTGCGAATAAATTGCGTCAAGAAATCGGTGCTATCCTTATACCTTCTTCTAATGATGGCCGCATAATGAGTGGCCGAGGGGTRCTTATTATTCGTTTGGGAGTATTTCCGTAGGGAAACCAACCGGACCCCGAAATCGA
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01924 Ps_contig_mira-and-tgicl-ass_franssen_17395
SEQUENCE=GGAATCCTTCTCCATTTCAACTATATATATATTCCACTTTCCCTYTCTTCTCTTCTGACCAATCTCTTTCTTTCCAACATTTCTCTCAGATCTACGTGAATGGTAGAAACCCTTTTCCCTTTCCCTTTCCCCTTCGATTTCATGCGCTCCTTTTCACCATCAACAACGGCGAGCGCTCTCTTCCCCTTCGCTTCTTGCATGCAAGGTTGGCACTATCGCTGCCTCGGTCTCGATCCATAGAAACCCTAATCCAACAATTTTCCACATTCTTTCAAAATTGATTCAATTTAGTAACTAGATTCTTGGGCGTAGTTTGTTGTTTGTGTTTT
TARGET=22,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01925 Ps_contig_mira-and-tgicl-ass_franssen_17401
SEQUENCE=GCTCTTTATATGGATGGTCATTACATYGGCTGATGGACCTTATCGTTTAGGGCCATAATTAACCATGGTTCATCATCATCATCACTTTATTTATTATATTATGTTATATTTTGTTATAGTTTAAGAGGGTATGTAAAACAATCTTATGATGTTCATTGTTGGATTTTAAATGTTACATGTTTGTAGCTATTGCTAGTTTTTTTTYCCYATCATTTTGTTACTTCTATCTTTTATTTTATGAGTTAATGTGAGCWAAACAGAAGATCGAAATTTCAAATTTRRAGGTAATTTAAG
TARGET=70,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01926 Ps_contig_mira-and-tgicl-ass_franssen_17402
SEQUENCE=TCTGCTACTAAGAAACTTCCAAATTGGTGANACTATGTTTTTGTAATACAATGCTCAGTTCCACAATGTGATGCGTGTGACACACGCTATGTACAAATCATGCAACGGATCGTCGCCTTTGACAACTTTCACCACTGGCAAAGACTCCATAAAGATAACAAACTATGGTCATCACTTTTTCTTGTGTGGTGTTCCTGGTCATTGTCAAGCAGGACAGAAGGTTGATATCAATGTGCTCAATGTTTCTGCTGCAGCAGCAGCAACACCAACTAAATCTCCGTCAGCATTGGCATCTCCGGTACCAGTTGCTAATACACCTGCACCTTCTCCTAACAATGCTTCGCCGTTGAATGTTGCAAAGGGAGCTTTTTGGTTTTTGTTGGTTTGGCCATGACAGTTTTTAGCAGTTTCTATTTCTAACTTTGCATAATAAGGAGCTGTTCTTATTTGTTGTTAGAGTTTGGTTTATGTCTTAAGGACTTGTCAAGGACTATTTTTTTTAATGGTTCATTATTATGATCCTTTATATAGATTGAAGGCATTGTTTCTATGATCCTTCATTTATTGTTTGTTTATTAGGTAAGTAAATTTAAAAAAGAAGTAATTTAAATAAAAGTTAAATTTTATAGTAAATTAAAAAGT
TARGET=251,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2560
PRIMER_LEFT_PENALTY=0.045112
PRIMER_RIGHT_PENALTY=0.210886
PRIMER_LEFT_SEQUENCE=TTTTTCTTGTGTGGTGTTCCTG
PRIMER_RIGHT_SEQUENCE=AAGCATTGTTAGGAGAAGGTGC
PRIMER_LEFT=175,22
PRIMER_RIGHT=340,22
PRIMER_LEFT_TM=60.045
PRIMER_RIGHT_TM=59.789
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.2617
PRIMER_LEFT_1_PENALTY=0.045112
PRIMER_RIGHT_1_PENALTY=0.216588
PRIMER_LEFT_1_SEQUENCE=TTTTTCTTGTGTGGTGTTCCTG
PRIMER_RIGHT_1_SEQUENCE=CGAAGCATTGTTAGGAGAAGGT
PRIMER_LEFT_1=175,22
PRIMER_RIGHT_1=342,22
PRIMER_LEFT_1_TM=60.045
PRIMER_RIGHT_1_TM=59.783
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=168
PRIMER_PAIR_PENALTY_2=0.3462
PRIMER_LEFT_2_PENALTY=0.045112
PRIMER_RIGHT_2_PENALTY=0.301100
PRIMER_LEFT_2_SEQUENCE=TTTTTCTTGTGTGGTGTTCCTG
PRIMER_RIGHT_2_SEQUENCE=TGCAGGTGTATTAGCAACTGGT
PRIMER_LEFT_2=175,22
PRIMER_RIGHT_2=321,22
PRIMER_LEFT_2_TM=60.045
PRIMER_RIGHT_2_TM=59.699
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=0.3854
PRIMER_LEFT_3_PENALTY=0.174518
PRIMER_RIGHT_3_PENALTY=0.210886
PRIMER_LEFT_3_SEQUENCE=ATTGTCAAGCAGGACAGAAGGT
PRIMER_RIGHT_3_SEQUENCE=AAGCATTGTTAGGAGAAGGTGC
PRIMER_LEFT_3=200,22
PRIMER_RIGHT_3=340,22
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=59.789
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=141
PRIMER_PAIR_PENALTY_4=0.3911
PRIMER_LEFT_4_PENALTY=0.174518
PRIMER_RIGHT_4_PENALTY=0.216588
PRIMER_LEFT_4_SEQUENCE=ATTGTCAAGCAGGACAGAAGGT
PRIMER_RIGHT_4_SEQUENCE=CGAAGCATTGTTAGGAGAAGGT
PRIMER_LEFT_4=200,22
PRIMER_RIGHT_4=342,22
PRIMER_LEFT_4_TM=60.175
PRIMER_RIGHT_4_TM=59.783
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_01927 Ps_contig_mira-and-tgicl-ass_franssen_17403
SEQUENCE=TGTCCAATTCTCGTTACTAGAGGATATCATTAGCATAAAGGATATATAACCCTAAGTTTTAAGTTCCATATTAGGTATCACTTGCATTTATTTTGGATTTGAAGATTTAGGGTTCGTACATTTTGGTCTTTGATTTGAGTTCTGTGGATAGAATTAGAGAAAGGAAACTATGGATTGGGAGATAGGAGGTAGAGACGATGGTTTTCATGGCAGGTTGGGGTCATTTCAGACTCCGCCGCCGCCGGAGGATATTTTCGGTGCGGCGGAGGAATAATCGGATTTCGCTTTAGGGAGAGAGAGGTTTAAGAAAGAAAGAGCTTGTGTACCCG
TARGET=233,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4512
PRIMER_LEFT_PENALTY=0.270774
PRIMER_RIGHT_PENALTY=0.180440
PRIMER_LEFT_SEQUENCE=TGGGAGATAGGAGGTAGAGACG
PRIMER_RIGHT_SEQUENCE=CGGGTACACAAGCTCTTTCTTT
PRIMER_LEFT=175,22
PRIMER_RIGHT=328,22
PRIMER_LEFT_TM=59.729
PRIMER_RIGHT_TM=59.820
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.7427
PRIMER_LEFT_1_PENALTY=0.562302
PRIMER_RIGHT_1_PENALTY=0.180440
PRIMER_LEFT_1_SEQUENCE=TGGATTGGGAGATAGGAGGTAG
PRIMER_RIGHT_1_SEQUENCE=CGGGTACACAAGCTCTTTCTTT
PRIMER_LEFT_1=170,22
PRIMER_RIGHT_1=328,22
PRIMER_LEFT_1_TM=59.438
PRIMER_RIGHT_1_TM=59.820
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.8699
PRIMER_LEFT_2_PENALTY=0.689440
PRIMER_RIGHT_2_PENALTY=0.180440
PRIMER_LEFT_2_SEQUENCE=GAAAGGAAACTATGGATTGGGA
PRIMER_RIGHT_2_SEQUENCE=CGGGTACACAAGCTCTTTCTTT
PRIMER_LEFT_2=158,22
PRIMER_RIGHT_2=328,22
PRIMER_LEFT_2_TM=59.311
PRIMER_RIGHT_2_TM=59.820
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.8946
PRIMER_LEFT_3_PENALTY=0.714145
PRIMER_RIGHT_3_PENALTY=0.180440
PRIMER_LEFT_3_SEQUENCE=GGGTTCGTACATTTTGGTCTTT
PRIMER_RIGHT_3_SEQUENCE=CGGGTACACAAGCTCTTTCTTT
PRIMER_LEFT_3=109,22
PRIMER_RIGHT_3=328,22
PRIMER_LEFT_3_TM=59.286
PRIMER_RIGHT_3_TM=59.820
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=0.9460
PRIMER_LEFT_4_PENALTY=0.270774
PRIMER_RIGHT_4_PENALTY=0.675181
PRIMER_LEFT_4_SEQUENCE=TGGGAGATAGGAGGTAGAGACG
PRIMER_RIGHT_4_SEQUENCE=CTCTCTCCCTAAAGCGAAATCC
PRIMER_LEFT_4=175,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=59.729
PRIMER_RIGHT_4_TM=60.675
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_01928 Ps_contig_mira-and-tgicl-ass_franssen_17403
SEQUENCE=TGTCCAATTCTCGTTACTAGAGGATATCATTAGCATAAAGGATATATAACCCTAAGTTTTAAGTTCCATATTAGGTATCACTTGCATTTATTTTGGATTTGAAGATTTAGGGTTCGTACATTTTGGTCTTTGATTTGAGTTCTGTGGATAGAATTAGAGAAAGGAAACTATGGATTGGGAGATAGGAGGTAGAGACGATGGTTTTCATGGCAGGTTGGGGTCATTTCAGACTCCGCCGCCGCCGGAGGATATTTTCGGTGCGGCGGAGGAATAATCGGATTTCGCTTTAGGGAGAGAGAGGTTTAAGAAAGAAAGAGCTTGTGTACCCG
TARGET=305,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01929 Ps_contig_mira-and-tgicl-ass_franssen_17416
SEQUENCE=AAAGTCAAAAAAAAAYMATTACAACATAAAAGAGCTCATAAATTGCCATTCAGTTGCTAATTAAAGACATAATACGTTTACTTCCATCATTAGTGGAAATAACAAATCCTAAACATAGACAAATTAGCATTGAATCATAAAGCCAAGGCAAATTAAGGAAAAGGATCAATCAACACAGTAGTAGTAAAGTTATGAAGCACCTTTTCATTTTAAAAACCTAATTGCAGCTTCAAAGGTGCAAGCAGCATTGTTCTTCCGATTCTCAGCATCTCCAAGTTGAAATCGAGATGAGATCCTTCCTTTGCCAGCATAACACGAGTGAATTCTGCTTCTTCTCGACGTGGAATCCCCTGCCGGGAATCCTCTGCACCAAACACTCGGCTTGTGACTCGGTAAAGTTACTAAACGTCAATGGTGAGAACCCCGACGACAAGAGAAGATTTTTCCAAGGAAGTGTTTGGTCCTGTGAACGCAGACGGCCCAAAACAAGCTTCTCAATGGCTGGTTGAAGATAGTATTTCTCGATCATTTGAAGTACATCTGGGTGAACGTTAACACTGACAGCGTCAAGTGACTCGAGCAAACCCGAATAAGACTGGATAGCGAGAATCATATGCTGAGGAAACGGTGCGTCGGTTCGATCGCAACTCCTATCCAACGTAACCACAATTTTTGGCATGAGCTGCTTCATGAATCGAAGCACCAAAGGAAGGTACGAAGGGTAATTTGAGAAAGAGCAAATAGGCAAGTTAACAACAACTGCTTCGCAGTCGCGAAGTGGCTGCGGCCATGATACAGAATTCAGAGAGTCGAGTGTCAGAATCTCAAGCTCAAAGGACATGTTAATTTCACCAGCATATTGTTTCAGATTCTCGTGAGTGAAACTAAGCTCGATTTCGTCGTGGTGAGAGGGTGAAACAAATGCAGTGATCTTAAGTGAAGGTGCACCGCCGTTCCTGAGTGCTAACTCCTGCATAAAAGAAGACCATTGTCCACCGAAACCGATATCAAAATCTACTATGTGAATTTGATCGAAACCTTCCACAGCTTCAAGCAATGCTTGGTTGGAAGTGAAATTAGCAAACTGCAGAACCGGTGAGATTTCTGAGAATGATTTATAAGCACCAATCTTGAGCAATAGCCCCGTAGGCGAAAAGCTGTTGTTGTTGTTGCTGTTGACATTCGATTGAAGAAGCAATTGTAATGCCTCCTTGAAATAGAAAGCAGCCCTTTGAAAAGGCTTCCCAATGGGAGAAATTTGGTGATTGAGCCGCGCCAATATCCCGTGCGCGAGTTCCGGATTACCGGCTTCTATAAGTTCTGCAGCCTTGAAAAGCTGATCAATTATAGCTTGCTGGAACTGATTGTTCCCTGCCGCATCTTCGCCGCCGCCGCTTCCAGGGTTCCCCATTTTCTGCTTCGGCGCCATTGGCCTCTGCTGCAGATGGTGTGGAAGTAGTTGAAGCTGCTGCTGATGATGATGCTGTTGTTGATGTTGCTGCCGCCTAAGAAACATCTCTTGACCAGAATCCAAAAATGGCATCTTCGGAATTTCATAATTATTTCCGCAATTGAGTCTTTTCGCCGGTGGCTGAG
TARGET=1159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01930 Ps_contig_mira-and-tgicl-ass_franssen_17416
SEQUENCE=AAAGTCAAAAAAAAAYMATTACAACATAAAAGAGCTCATAAATTGCCATTCAGTTGCTAATTAAAGACATAATACGTTTACTTCCATCATTAGTGGAAATAACAAATCCTAAACATAGACAAATTAGCATTGAATCATAAAGCCAAGGCAAATTAAGGAAAAGGATCAATCAACACAGTAGTAGTAAAGTTATGAAGCACCTTTTCATTTTAAAAACCTAATTGCAGCTTCAAAGGTGCAAGCAGCATTGTTCTTCCGATTCTCAGCATCTCCAAGTTGAAATCGAGATGAGATCCTTCCTTTGCCAGCATAACACGAGTGAATTCTGCTTCTTCTCGACGTGGAATCCCCTGCCGGGAATCCTCTGCACCAAACACTCGGCTTGTGACTCGGTAAAGTTACTAAACGTCAATGGTGAGAACCCCGACGACAAGAGAAGATTTTTCCAAGGAAGTGTTTGGTCCTGTGAACGCAGACGGCCCAAAACAAGCTTCTCAATGGCTGGTTGAAGATAGTATTTCTCGATCATTTGAAGTACATCTGGGTGAACGTTAACACTGACAGCGTCAAGTGACTCGAGCAAACCCGAATAAGACTGGATAGCGAGAATCATATGCTGAGGAAACGGTGCGTCGGTTCGATCGCAACTCCTATCCAACGTAACCACAATTTTTGGCATGAGCTGCTTCATGAATCGAAGCACCAAAGGAAGGTACGAAGGGTAATTTGAGAAAGAGCAAATAGGCAAGTTAACAACAACTGCTTCGCAGTCGCGAAGTGGCTGCGGCCATGATACAGAATTCAGAGAGTCGAGTGTCAGAATCTCAAGCTCAAAGGACATGTTAATTTCACCAGCATATTGTTTCAGATTCTCGTGAGTGAAACTAAGCTCGATTTCGTCGTGGTGAGAGGGTGAAACAAATGCAGTGATCTTAAGTGAAGGTGCACCGCCGTTCCTGAGTGCTAACTCCTGCATAAAAGAAGACCATTGTCCACCGAAACCGATATCAAAATCTACTATGTGAATTTGATCGAAACCTTCCACAGCTTCAAGCAATGCTTGGTTGGAAGTGAAATTAGCAAACTGCAGAACCGGTGAGATTTCTGAGAATGATTTATAAGCACCAATCTTGAGCAATAGCCCCGTAGGCGAAAAGCTGTTGTTGTTGTTGCTGTTGACATTCGATTGAAGAAGCAATTGTAATGCCTCCTTGAAATAGAAAGCAGCCCTTTGAAAAGGCTTCCCAATGGGAGAAATTTGGTGATTGAGCCGCGCCAATATCCCGTGCGCGAGTTCCGGATTACCGGCTTCTATAAGTTCTGCAGCCTTGAAAAGCTGATCAATTATAGCTTGCTGGAACTGATTGTTCCCTGCCGCATCTTCGCCGCCGCCGCTTCCAGGGTTCCCCATTTTCTGCTTCGGCGCCATTGGCCTCTGCTGCAGATGGTGTGGAAGTAGTTGAAGCTGCTGCTGATGATGATGCTGTTGTTGATGTTGCTGCCGCCTAAGAAACATCTCTTGACCAGAATCCAAAAATGGCATCTTCGGAATTTCATAATTATTTCCGCAATTGAGTCTTTTCGCCGGTGGCTGAG
TARGET=1384,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01931 Ps_contig_mira-and-tgicl-ass_franssen_17420
SEQUENCE=ATAGTAGATTCTTCTCCGTGAAGCTTTCTGACCGGTGGACGCAGCAGCAGCAGCGTTCGGCGTTGCCGTATCTTGTTGAGATTGCGGATTGTGATGATGTTGAGGTTTATGTGGAGACGTTGAGGTTGATGTATTGTAAGGATCTTAGGAAGAAGCTTATGAAAGAGGATGTTTNTAAGGTTTGGTATCTTAAAGGTTTCGGCTGCAATTGGGTTTGATGGTTCCGGGG
TARGET=41,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5787
PRIMER_LEFT_PENALTY=0.548390
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=CTTCTCCGTGAAGCTTTCTGAC
PRIMER_RIGHT_SEQUENCE=CGTCTCCACATAAACCTCAACA
PRIMER_LEFT=10,22
PRIMER_RIGHT=118,22
PRIMER_LEFT_TM=60.548
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=0.5787
PRIMER_LEFT_1_PENALTY=0.548390
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=CTTCTCCGTGAAGCTTTCTGAC
PRIMER_RIGHT_1_SEQUENCE=CAACCTCAACGTCTCCACATAA
PRIMER_LEFT_1=10,22
PRIMER_RIGHT_1=127,22
PRIMER_LEFT_1_TM=60.548
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=0.5787
PRIMER_LEFT_2_PENALTY=0.548390
PRIMER_RIGHT_2_PENALTY=0.030276
PRIMER_LEFT_2_SEQUENCE=CTTCTCCGTGAAGCTTTCTGAC
PRIMER_RIGHT_2_SEQUENCE=CAACGTCTCCACATAAACCTCA
PRIMER_LEFT_2=10,22
PRIMER_RIGHT_2=121,22
PRIMER_LEFT_2_TM=60.548
PRIMER_RIGHT_2_TM=60.030
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=1.0284
PRIMER_LEFT_3_PENALTY=0.548390
PRIMER_RIGHT_3_PENALTY=0.480018
PRIMER_LEFT_3_SEQUENCE=CTTCTCCGTGAAGCTTTCTGAC
PRIMER_RIGHT_3_SEQUENCE=GCCGAAACCTTTAAGATACCAA
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=202,22
PRIMER_LEFT_3_TM=60.548
PRIMER_RIGHT_3_TM=59.520
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=1.0531
PRIMER_LEFT_4_PENALTY=1.022866
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=TTCTTCTCCGTGAAGCTTTCTG
PRIMER_RIGHT_4_SEQUENCE=CAACCTCAACGTCTCCACATAA
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=127,22
PRIMER_LEFT_4_TM=61.023
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_01932 Ps_contig_mira-and-tgicl-ass_franssen_17426
SEQUENCE=CCGTTCTTGGTGTTGGTGGAGAGTTATTACTACAAGGTAGTCCTAACTCTTTTCTAGCTTTGAAAATGAGAGAGTGAATAAGTGATTCAGGACCAAATGCTAACCTTATCATTTCCTTCTCGCCATGGTCTTGAATGAGAAGTACACCCATGATTTTAGATGCATTTTCAGGGTCCATTTTTTGGATCCTTGAAAACACTGTCCTAGTAGCTTCATAACCATCCATGACTCACTAGTTTGGTTTGGTTTGGTTTCAAGAACAAACTAGGTTATCCTTATATTACTTTCAAACTCCTATTACAAGTAAAGTTTGTCTGTGAAAGCCCTACCTACCAAGGTGAGATGAGTATGTTTCTTTCTTTCACATATTCCTGCAAACCATAAAGAAGAAAAAGGAGAAGGGAAAGTAAGAAAGTGAAGTGTTAAAAGTAGTATTCTCGTC
TARGET=236,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0442
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=AAGTGATTCAGGACCAAATGCT
PRIMER_RIGHT_SEQUENCE=GGTAGGGCTTTCACAGACAAAC
PRIMER_LEFT=79,22
PRIMER_RIGHT=329,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.1250
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.120688
PRIMER_LEFT_1_SEQUENCE=AAGTGATTCAGGACCAAATGCT
PRIMER_RIGHT_1_SEQUENCE=CATCTCACCTTGGTAGGTAGGG
PRIMER_LEFT_1=79,22
PRIMER_RIGHT_1=344,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.879
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.1689
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.164560
PRIMER_LEFT_2_SEQUENCE=AAGTGATTCAGGACCAAATGCT
PRIMER_RIGHT_2_SEQUENCE=AGGTAGGGCTTTCACAGACAAA
PRIMER_LEFT_2=79,22
PRIMER_RIGHT_2=330,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=60.165
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=0.1986
PRIMER_LEFT_3_PENALTY=0.158697
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=GATTCAGGACCAAATGCTAACC
PRIMER_RIGHT_3_SEQUENCE=GGTAGGGCTTTCACAGACAAAC
PRIMER_LEFT_3=83,22
PRIMER_RIGHT_3=329,22
PRIMER_LEFT_3_TM=59.841
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.2794
PRIMER_LEFT_4_PENALTY=0.158697
PRIMER_RIGHT_4_PENALTY=0.120688
PRIMER_LEFT_4_SEQUENCE=GATTCAGGACCAAATGCTAACC
PRIMER_RIGHT_4_SEQUENCE=CATCTCACCTTGGTAGGTAGGG
PRIMER_LEFT_4=83,22
PRIMER_RIGHT_4=344,22
PRIMER_LEFT_4_TM=59.841
PRIMER_RIGHT_4_TM=59.879
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_01933 Ps_contig_mira-and-tgicl-ass_franssen_17426
SEQUENCE=CCGTTCTTGGTGTTGGTGGAGAGTTATTACTACAAGGTAGTCCTAACTCTTTTCTAGCTTTGAAAATGAGAGAGTGAATAAGTGATTCAGGACCAAATGCTAACCTTATCATTTCCTTCTCGCCATGGTCTTGAATGAGAAGTACACCCATGATTTTAGATGCATTTTCAGGGTCCATTTTTTGGATCCTTGAAAACACTGTCCTAGTAGCTTCATAACCATCCATGACTCACTAGTTTGGTTTGGTTTGGTTTCAAGAACAAACTAGGTTATCCTTATATTACTTTCAAACTCCTATTACAAGTAAAGTTTGTCTGTGAAAGCCCTACCTACCAAGGTGAGATGAGTATGTTTCTTTCTTTCACATATTCCTGCAAACCATAAAGAAGAAAAAGGAGAAGGGAAAGTAAGAAAGTGAAGTGTTAAAAGTAGTATTCTCGTC
TARGET=352,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5177
PRIMER_LEFT_PENALTY=0.237699
PRIMER_RIGHT_PENALTY=2.279986
PRIMER_LEFT_SEQUENCE=GGTTTGGTTTGGTTTCAAGAAC
PRIMER_RIGHT_SEQUENCE=CTTTCCCTTCTCCTTTTTCTTC
PRIMER_LEFT=239,22
PRIMER_RIGHT=406,22
PRIMER_LEFT_TM=59.762
PRIMER_RIGHT_TM=57.720
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=0.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=2.5922
PRIMER_LEFT_1_PENALTY=0.312177
PRIMER_RIGHT_1_PENALTY=2.279986
PRIMER_LEFT_1_SEQUENCE=TTTAGATGCATTTTCAGGGTCC
PRIMER_RIGHT_1_SEQUENCE=CTTTCCCTTCTCCTTTTTCTTC
PRIMER_LEFT_1=154,22
PRIMER_RIGHT_1=406,22
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=57.720
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=2.7023
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=2.662448
PRIMER_LEFT_2_SEQUENCE=GTTTGTCTGTGAAAGCCCTACC
PRIMER_RIGHT_2_SEQUENCE=TCACTTTCTTACTTTCCCTTCTCC
PRIMER_LEFT_2=308,22
PRIMER_RIGHT_2=417,24
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=59.338
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=110
PRIMER_PAIR_PENALTY_3=2.7849
PRIMER_LEFT_3_PENALTY=0.504940
PRIMER_RIGHT_3_PENALTY=2.279986
PRIMER_LEFT_3_SEQUENCE=GCCATGGTCTTGAATGAGAAGT
PRIMER_RIGHT_3_SEQUENCE=CTTTCCCTTCTCCTTTTTCTTC
PRIMER_LEFT_3=121,22
PRIMER_RIGHT_3=406,22
PRIMER_LEFT_3_TM=60.505
PRIMER_RIGHT_3_TM=57.720
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=2.7959
PRIMER_LEFT_4_PENALTY=0.515892
PRIMER_RIGHT_4_PENALTY=2.279986
PRIMER_LEFT_4_SEQUENCE=GGATCCTTGAAAACACTGTCCT
PRIMER_RIGHT_4_SEQUENCE=CTTTCCCTTCTCCTTTTTCTTC
PRIMER_LEFT_4=183,22
PRIMER_RIGHT_4=406,22
PRIMER_LEFT_4_TM=59.484
PRIMER_RIGHT_4_TM=57.720
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_01934 Ps_contig_mira-and-tgicl-ass_franssen_17436
SEQUENCE=TGGATCTTCTTAGTTTTTGCTCTATTATTAACATTGTTTTATTCCATCAACAACGAAACCARCAACAACAACAACAAGAACCATTCATTTAGGTTACTCAGACAACAGCAGCAACAGCAACAACAA
TARGET=63,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01935 Ps_contig_mira-and-tgicl-ass_franssen_17437
SEQUENCE=GTTGAAACTACGTTGTTGTTGCTGTTGCTGCTGTTGTCTGAGTAACCTAAATGAATGSTTSTTGTTGTTGTTGTTGGTTTCGTTGTTGATGGAATAAAACAATGTTAATAATAGAGCAAAAACTAAGAAGATCCACAACAACAAAATTGAAGCTCTTGTTAAAGAAAGTCTCTTCTCTCTTGTCCTTTCTCTTTCTCTGAAACCTATCATTGCACTTTCTGTTGTGGATTTTGTCACACACAGACACGAGAAAAGAAGAAGCTTCAGAAATCTACCATGAAATTGTGCACAGAAAGATATGTCTCTCTCTCTCTCACTCTTGATTTTGAGTTGAACACAAAGTGTGATTTGGATAATTCTGAAAGAAAGAGAGGTAGAAAAGTAGAAAGAAAGAGACTGAAAAC
TARGET=62,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01936 Ps_contig_mira-and-tgicl-ass_franssen_17437
SEQUENCE=GTTGAAACTACGTTGTTGTTGCTGTTGCTGCTGTTGTCTGAGTAACCTAAATGAATGSTTSTTGTTGTTGTTGTTGGTTTCGTTGTTGATGGAATAAAACAATGTTAATAATAGAGCAAAAACTAAGAAGATCCACAACAACAAAATTGAAGCTCTTGTTAAAGAAAGTCTCTTCTCTCTTGTCCTTTCTCTTTCTCTGAAACCTATCATTGCACTTTCTGTTGTGGATTTTGTCACACACAGACACGAGAAAAGAAGAAGCTTCAGAAATCTACCATGAAATTGTGCACAGAAAGATATGTCTCTCTCTCTCTCACTCTTGATTTTGAGTTGAACACAAAGTGTGATTTGGATAATTCTGAAAGAAAGAGAGGTAGAAAAGTAGAAAGAAAGAGACTGAAAAC
TARGET=302,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01937 Ps_contig_mira-and-tgicl-ass_franssen_17468
SEQUENCE=AGTACGCGGGGAAAACAACCTCATGTTCTTAAAGTGCTTTCTCATAAATAAATAAAAACTTAAATTTCTAGAAATTGAACTTCCTTTTATTTCATCAAGAAATCTTCACACACACTAATGAGTTCTAAGATAAACTTCTCAAGCCCACCAATACCATGCAAATTCCACACCAAAAAACCAAATCCTCTAAAAAGAAACACTGTCATCTTCTCTTCCTTTTCTTCAAGTGAAAAACAACAAAAAATCATTTCCACCATAATTGAAACTCCATCAAATGATAATAAGGCTTACACTATCAACTTCAAGACAAAAAAGTGCTTGCAAGCTTGGAATTTCAAGGTATCCTGATTTTGAATATGATGCTGAAGGAGGAATAGGAAATGGTTTTGGTGCAAAGGCAAAAAAATCAAGATGATAATGATATTATTCTTGTTTCGTTTGATCTTGAAACATTGTATATTCCATCGTTAACAAGCTCTACTACTAAGTTTCTTGGATTGTCATTGCCCACCATTCTTGGAGGATTGATATTTGTTCCAAGAAGTCTTTCCATGGAAGTATTAATCAAGAATCTGGCAAGGTTGAT
TARGET=44,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5848
PRIMER_LEFT_PENALTY=1.584033
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=GGGGAAAACAACCTCATGTTC
PRIMER_RIGHT_SEQUENCE=TTGGTGGGCTTGAGAAGTTTAT
PRIMER_LEFT=7,21
PRIMER_RIGHT=150,22
PRIMER_LEFT_TM=60.584
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=1.5848
PRIMER_LEFT_1_PENALTY=1.584033
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=GGGGAAAACAACCTCATGTTC
PRIMER_RIGHT_1_SEQUENCE=ATTGGTGGGCTTGAGAAGTTTA
PRIMER_LEFT_1=7,21
PRIMER_RIGHT_1=151,22
PRIMER_LEFT_1_TM=60.584
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=1.5848
PRIMER_LEFT_2_PENALTY=1.584033
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=GGGGAAAACAACCTCATGTTC
PRIMER_RIGHT_2_SEQUENCE=TATTGGTGGGCTTGAGAAGTTT
PRIMER_LEFT_2=7,21
PRIMER_RIGHT_2=152,22
PRIMER_LEFT_2_TM=60.584
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=1.7010
PRIMER_LEFT_3_PENALTY=1.584033
PRIMER_RIGHT_3_PENALTY=0.116923
PRIMER_LEFT_3_SEQUENCE=GGGGAAAACAACCTCATGTTC
PRIMER_RIGHT_3_SEQUENCE=GTGTGGAATTTGCATGGTATTG
PRIMER_LEFT_3=7,21
PRIMER_RIGHT_3=169,22
PRIMER_LEFT_3_TM=60.584
PRIMER_RIGHT_3_TM=60.117
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=163
PRIMER_PAIR_PENALTY_4=2.0579
PRIMER_LEFT_4_PENALTY=1.584033
PRIMER_RIGHT_4_PENALTY=0.473868
PRIMER_LEFT_4_SEQUENCE=GGGGAAAACAACCTCATGTTC
PRIMER_RIGHT_4_SEQUENCE=GTATTGGTGGGCTTGAGAAGTT
PRIMER_LEFT_4=7,21
PRIMER_RIGHT_4=153,22
PRIMER_LEFT_4_TM=60.584
PRIMER_RIGHT_4_TM=59.526
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_01938 Ps_contig_mira-and-tgicl-ass_franssen_17481
SEQUENCE=GAAWMGGRGRKTTGGTAAACGAAAATAAAAAGAACGAAGGAAAGCGAAGGCTTTTGTAGCGTTCCTCCGCCATCGTCAACGCAGCGGGAGTGATGAGTTCCGCCGATACATTCAATGGGGGTTCTGTACAATCTGTCAAAGAATCTGTTCCTGGGGCAAGCATTGAAGCAAAACAGGAAGTTACCGAGGTGAAAGCTCAAGTCGATGCTATGTGGGAACAAATGAATAAAGGAGTATCAAATAAAGTTATTAGCAGTTTTACAAGCAAGCCTAAATCTACCTCAAAAAGCAATCCAAAGAAGAGATCTTCTAACTGGATGTCTTATTTGGGGTTGGCACCAAAGGCAACTGAATCTATTGTGCAAGGTGCTTCGCAGAATGAACCTGGTGTGCTGCAAAACAGTGCAAGTGATGAAGCTAAGAAGTTTGCTGCTGCTGCTCTTGCTGCGGTGAAAGTTGATGCTGCCGCTTCTGCGTCAAGCAGGGGGAAACTTGTGATTACCGAGGTTCGAGACTTTGCTGGTCAAGAAATTGAATATAAGAAGCTCGTAGATTCGGATTCAAAGGAGGCAATTGAAAGAGCCAAAGCCCCTGCACCTTCTGCCGTGGATGCTGTCCTTGAACAGATAAAAAAGAAATCTAAGCTCAGTGTACTTGATAAGACCAAAAAAGACTGGGGAGAGTTTAAGGAAGAAAATAAGGGACTGGAAAATGAGCTTGATGTTTACAAGAAGAGTTCAAACCAGTATTTGGATAAGGTTTCCTTCTTGCAGCGAACAGATTACCGTGAATATGAGCGAGAGAGAGATGCACGACTGGCTTTGCAATCCAGGAAGCGACCAGATATGCAAGATGATCCTGTATAACTGAAGAAAGCTGAAGTGTTGAGTTGCTACCTTTGGCAAATTATTTATGGTTATATCATGAGTTCATATCTAGGTGTTCCTGGGAATTTCTTGGTCTCCAGAAGAAAAAAAGGATCTTTGGTCACATTAGTAGGTGATGATGCATTTTGAGGTTCAAGGTTTAGAATGCTTTCAAATGATGTGGTACTTGACTTGGATGAGAAAAACGGGACTAAAAACCCAAGTGTAAATTTTAGACTGACATTAGCACAACTCACTGAAGTTACTAGTTTTCAAGAAGCATTGTATTGGTCAATTTTCTTGAAAAAAAAAGATTCGCTGTTTATCTGTCAACTCACTGAAGTTACTTAGTTTTTATGTGCTGTACCAGTAAACTTTTAACTTAATGAATTGATGGCAGCCAACCCTCMAMCMWMWAAYYYTTAAG
TARGET=428,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01939 Ps_contig_mira-and-tgicl-ass_franssen_17481
SEQUENCE=GAAWMGGRGRKTTGGTAAACGAAAATAAAAAGAACGAAGGAAAGCGAAGGCTTTTGTAGCGTTCCTCCGCCATCGTCAACGCAGCGGGAGTGATGAGTTCCGCCGATACATTCAATGGGGGTTCTGTACAATCTGTCAAAGAATCTGTTCCTGGGGCAAGCATTGAAGCAAAACAGGAAGTTACCGAGGTGAAAGCTCAAGTCGATGCTATGTGGGAACAAATGAATAAAGGAGTATCAAATAAAGTTATTAGCAGTTTTACAAGCAAGCCTAAATCTACCTCAAAAAGCAATCCAAAGAAGAGATCTTCTAACTGGATGTCTTATTTGGGGTTGGCACCAAAGGCAACTGAATCTATTGTGCAAGGTGCTTCGCAGAATGAACCTGGTGTGCTGCAAAACAGTGCAAGTGATGAAGCTAAGAAGTTTGCTGCTGCTGCTCTTGCTGCGGTGAAAGTTGATGCTGCCGCTTCTGCGTCAAGCAGGGGGAAACTTGTGATTACCGAGGTTCGAGACTTTGCTGGTCAAGAAATTGAATATAAGAAGCTCGTAGATTCGGATTCAAAGGAGGCAATTGAAAGAGCCAAAGCCCCTGCACCTTCTGCCGTGGATGCTGTCCTTGAACAGATAAAAAAGAAATCTAAGCTCAGTGTACTTGATAAGACCAAAAAAGACTGGGGAGAGTTTAAGGAAGAAAATAAGGGACTGGAAAATGAGCTTGATGTTTACAAGAAGAGTTCAAACCAGTATTTGGATAAGGTTTCCTTCTTGCAGCGAACAGATTACCGTGAATATGAGCGAGAGAGAGATGCACGACTGGCTTTGCAATCCAGGAAGCGACCAGATATGCAAGATGATCCTGTATAACTGAAGAAAGCTGAAGTGTTGAGTTGCTACCTTTGGCAAATTATTTATGGTTATATCATGAGTTCATATCTAGGTGTTCCTGGGAATTTCTTGGTCTCCAGAAGAAAAAAAGGATCTTTGGTCACATTAGTAGGTGATGATGCATTTTGAGGTTCAAGGTTTAGAATGCTTTCAAATGATGTGGTACTTGACTTGGATGAGAAAAACGGGACTAAAAACCCAAGTGTAAATTTTAGACTGACATTAGCACAACTCACTGAAGTTACTAGTTTTCAAGAAGCATTGTATTGGTCAATTTTCTTGAAAAAAAAAGATTCGCTGTTTATCTGTCAACTCACTGAAGTTACTTAGTTTTTATGTGCTGTACCAGTAAACTTTTAACTTAATGAATTGATGGCAGCCAACCCTCMAMCMWMWAAYYYTTAAG
TARGET=799,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01940 Ps_contig_mira-and-tgicl-ass_franssen_17484
SEQUENCE=CCCTGTCCCTGCTTCTCTCCCTGTCCCTGTCACGGCTCCTCTCCCTGTCTCGTCCTTTATCCCTTTTCCTCTCCTTATCTCTCCCATTCTCCAGCTCCTTTTCTCTACTTCTCGAGGATTCTCTTTCATTATCACGAGAACCATATTGAGATTTGAAGTCGGTGAGATCGTCGTCAGCAGCAGCAGGTGGTTGTGGAGAGGAATCATTCTGATGATTATGATTGTGATCTTCTTCTTCTTCTGGTTCGTATTTTCTCTCTTCGAATTCGTATTCACCCATTTTTTTTTCTGTTCTTCACTTTCAAATGAATTCGATTCTCCCTGCGTA
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0660
PRIMER_LEFT_PENALTY=0.031944
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=TCCTTATCTCTCCCATTCTCCA
PRIMER_RIGHT_SEQUENCE=GGGTGAATACGAATTCGAAGAG
PRIMER_LEFT=71,22
PRIMER_RIGHT=277,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.0688
PRIMER_LEFT_1_PENALTY=0.034674
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=CTCGTCCTTTATCCCTTTTCCT
PRIMER_RIGHT_1_SEQUENCE=GGGTGAATACGAATTCGAAGAG
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=59.965
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.1814
PRIMER_LEFT_2_PENALTY=0.147295
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=TTGAGATTTGAAGTCGGTGAGA
PRIMER_RIGHT_2_SEQUENCE=GGGTGAATACGAATTCGAAGAG
PRIMER_LEFT_2=145,22
PRIMER_RIGHT_2=277,22
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=133
PRIMER_PAIR_PENALTY_3=0.2233
PRIMER_LEFT_3_PENALTY=0.189235
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=TATCTCTCCCATTCTCCAGCTC
PRIMER_RIGHT_3_SEQUENCE=GGGTGAATACGAATTCGAAGAG
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=277,22
PRIMER_LEFT_3_TM=59.811
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.3213
PRIMER_LEFT_4_PENALTY=0.287182
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=TCTCCCTGTCTCGTCCTTTATC
PRIMER_RIGHT_4_SEQUENCE=GGGTGAATACGAATTCGAAGAG
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=277,22
PRIMER_LEFT_4_TM=59.713
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_01941 Ps_contig_mira-and-tgicl-ass_franssen_17484
SEQUENCE=CCCTGTCCCTGCTTCTCTCCCTGTCCCTGTCACGGCTCCTCTCCCTGTCTCGTCCTTTATCCCTTTTCCTCTCCTTATCTCTCCCATTCTCCAGCTCCTTTTCTCTACTTCTCGAGGATTCTCTTTCATTATCACGAGAACCATATTGAGATTTGAAGTCGGTGAGATCGTCGTCAGCAGCAGCAGGTGGTTGTGGAGAGGAATCATTCTGATGATTATGATTGTGATCTTCTTCTTCTTCTGGTTCGTATTTTCTCTCTTCGAATTCGTATTCACCCATTTTTTTTTCTGTTCTTCACTTTCAAATGAATTCGATTCTCCCTGCGTA
TARGET=228,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0660
PRIMER_LEFT_PENALTY=0.031944
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=TCCTTATCTCTCCCATTCTCCA
PRIMER_RIGHT_SEQUENCE=GGGTGAATACGAATTCGAAGAG
PRIMER_LEFT=71,22
PRIMER_RIGHT=277,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.0688
PRIMER_LEFT_1_PENALTY=0.034674
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=CTCGTCCTTTATCCCTTTTCCT
PRIMER_RIGHT_1_SEQUENCE=GGGTGAATACGAATTCGAAGAG
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=59.965
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.1814
PRIMER_LEFT_2_PENALTY=0.147295
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=TTGAGATTTGAAGTCGGTGAGA
PRIMER_RIGHT_2_SEQUENCE=GGGTGAATACGAATTCGAAGAG
PRIMER_LEFT_2=145,22
PRIMER_RIGHT_2=277,22
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=133
PRIMER_PAIR_PENALTY_3=0.2233
PRIMER_LEFT_3_PENALTY=0.189235
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=TATCTCTCCCATTCTCCAGCTC
PRIMER_RIGHT_3_SEQUENCE=GGGTGAATACGAATTCGAAGAG
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=277,22
PRIMER_LEFT_3_TM=59.811
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.3213
PRIMER_LEFT_4_PENALTY=0.287182
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=TCTCCCTGTCTCGTCCTTTATC
PRIMER_RIGHT_4_SEQUENCE=GGGTGAATACGAATTCGAAGAG
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=277,22
PRIMER_LEFT_4_TM=59.713
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_01942 Ps_contig_mira-and-tgicl-ass_franssen_17485
SEQUENCE=CGTCCTTTATCCCTTTTCCTCTCCTTATCTCTCCCATTCTCCAGCTCCTTTTCTCTACTTCTCGAGGATTCTCTTTCATTATCACGAGAACCATAGTTAGATTTGAAGTCGGTGAGATCGTCGTCAGCAGCAGCAGGTGGTTGTGGAGAGGAATCATTCTGATGATTATGATTGTGATCTTCTTCTTCTTCTTCTGGTTCGTTATTTTCTCTCTTCGAATTCGTATTCCAYCCTWTTTTTTTTCTGTTCTTCACTTTCAAATGAACTTCCCCCG
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01943 Ps_contig_mira-and-tgicl-ass_franssen_17485
SEQUENCE=CGTCCTTTATCCCTTTTCCTCTCCTTATCTCTCCCATTCTCCAGCTCCTTTTCTCTACTTCTCGAGGATTCTCTTTCATTATCACGAGAACCATAGTTAGATTTGAAGTCGGTGAGATCGTCGTCAGCAGCAGCAGGTGGTTGTGGAGAGGAATCATTCTGATGATTATGATTGTGATCTTCTTCTTCTTCTTCTGGTTCGTTATTTTCTCTCTTCGAATTCGTATTCCAYCCTWTTTTTTTTCTGTTCTTCACTTTCAAATGAACTTCCCCCG
TARGET=178,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01944 Ps_contig_mira-and-tgicl-ass_franssen_17494
SEQUENCE=CATCCTGGAACCAATTTCGGGGAGATTTTAGTCTAATGTTCAAGGTTCGCTGCCCAAAACATTGCACCAATGATGACCACAAGTTCACCTGCGCCTCATAGCTGCACAAGTCCCAACAAAAACCTATTTTACAAATTCTAGGGAAAACNGACAAACACAACCGNAAATAGAGAGAGAGAGAAGGANAGAANGGAGAGAAGTGTGGCGTGNGNTGTCGTTGGTGGTCTT
TARGET=169,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.7658
PRIMER_LEFT_PENALTY=0.276812
PRIMER_RIGHT_PENALTY=2.488938
PRIMER_LEFT_SEQUENCE=CAATGATGACCACAAGTTCACC
PRIMER_RIGHT_SEQUENCE=ACGCCACACTTCTCTCCNTT
PRIMER_LEFT=67,22
PRIMER_RIGHT=207,20
PRIMER_LEFT_TM=60.277
PRIMER_RIGHT_TM=60.489
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=52.632
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.1250
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=2.7658
PRIMER_LEFT_1_PENALTY=0.276812
PRIMER_RIGHT_1_PENALTY=2.488938
PRIMER_LEFT_1_SEQUENCE=CCAATGATGACCACAAGTTCAC
PRIMER_RIGHT_1_SEQUENCE=ACGCCACACTTCTCTCCNTT
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=207,20
PRIMER_LEFT_1_TM=60.277
PRIMER_RIGHT_1_TM=60.489
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.632
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.1250
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=142
PRIMER_PAIR_PENALTY_2=2.7658
PRIMER_LEFT_2_PENALTY=0.276812
PRIMER_RIGHT_2_PENALTY=2.488938
PRIMER_LEFT_2_SEQUENCE=CACCAATGATGACCACAAGTTC
PRIMER_RIGHT_2_SEQUENCE=ACGCCACACTTCTCTCCNTT
PRIMER_LEFT_2=64,22
PRIMER_RIGHT_2=207,20
PRIMER_LEFT_2_TM=60.277
PRIMER_RIGHT_2_TM=60.489
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.632
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.1250
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=2.7658
PRIMER_LEFT_3_PENALTY=0.276812
PRIMER_RIGHT_3_PENALTY=2.488938
PRIMER_LEFT_3_SEQUENCE=ACCAATGATGACCACAAGTTCA
PRIMER_RIGHT_3_SEQUENCE=ACGCCACACTTCTCTCCNTT
PRIMER_LEFT_3=65,22
PRIMER_RIGHT_3=207,20
PRIMER_LEFT_3_TM=60.277
PRIMER_RIGHT_3_TM=60.489
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.632
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.1250
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=3.0082
PRIMER_LEFT_4_PENALTY=0.519213
PRIMER_RIGHT_4_PENALTY=2.488938
PRIMER_LEFT_4_SEQUENCE=ACCAATTTCGGGGAGATTTTAG
PRIMER_RIGHT_4_SEQUENCE=ACGCCACACTTCTCTCCNTT
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=207,20
PRIMER_LEFT_4_TM=60.519
PRIMER_RIGHT_4_TM=60.489
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.632
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=9.1250
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_01945 Ps_contig_mira-and-tgicl-ass_franssen_17499
SEQUENCE=TGGAAGAAATGTGTGTGTGTGTGGAATTGACAAGAACTGTGGAAAGTGTAATTTCTAAGGATGATGTGAAGAAGGTTATKAAAATAGTTATGGATCAAGAAGGAAAGGGAAAAGAGATGAAGGAGAAGGCAAATCAGATTGCAGTATACATGAGAGAGGCAACAATAGAAAGAGGTGAAGAAAAGGGGTCTTCATTGAGAGCAATTGATGATTTTGTGAGAACCATTTTACAAATATAAGGTTCTATGTGTTGTTGCATTTGAATTTGACAATTACTTGTAATAAACTAATC
TARGET=10,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01946 Ps_contig_mira-and-tgicl-ass_franssen_17529
SEQUENCE=GTCGGGGGTCCCTTCTCTCACGCGACGATCTTCTTCTTCCTCCTGGAAAAACCGGCGAAACCGGTGTTACCTCCACACCACAACGACACAAATCCCACCTTCCCTTATTCTTCCCCAAACAACCACCACCGTTCCTTTTTCTTCTTACTCCATCACCCTCACCTTCATCCACCCTCACGAGCTACTCCCTCCGGCCACCGCAAACAGCTCACCATCACCATCTTATCTTCTTCTTCCCCACAACTTCACTGGAAAACGTCGCGATGCAACAATGACTCCACCATCGAAACCCACATCAATTTCGCCGGTGAATCCAAACCACCACCGTCATTCTTCTTCTTCCGACACCAAATCGCAACCGTCACGCACCACTATACACCTCCAATCAATTGTTTTCACTAAATCCTTTCAATCTTCAACAAGCTCATAACCATACCGACCCACTCCCTTCAAAGGTCACCATCGAAGCAACCTCACTTCACCAGAATCATCAAACCCTTTCACACCATCACTTGTATTCACGCATTTCGATTCTAGAAAACACACACGCATCAGCAGTGCATATTCTTTCAAACCATGTGCACACAACCGAACTTCCCATCGACAACCTTGATTCACCATTATATCAGGCTTGCAACAACTGGAATTTGGCCTCAACAATTGGGCAAATTATGAAACATTTGAGTTTGAAATGCTTGGTTGGTAAGCTAAAGGATGATTCAATGTTGTTATGGTAACCACCCGACGTCACGGTAACGATCCATGATCCGAATTCAGCTCTGGTTCGCAACAACAATAAACTCAGGTAGCAACATTAATAGTAGCGGTAACGGTGGAAAAATTAACGAAAACCTCAACATTGTTTGGTATTATTTCATTTATGAGATTTGGTTTCAGTTTGTTTATGTGTTGATGTTTTGACTTTGGATAAGCGTTGCAGCTTTTGAATTTTATTGTTGCTCTGGTTTCAATGTGTATTTAGTTTTATGATCCTAATTTTAGAAAACC
TARGET=331,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2413
PRIMER_LEFT_PENALTY=0.145769
PRIMER_RIGHT_PENALTY=0.095574
PRIMER_LEFT_SEQUENCE=TCTTCTTCCCCACAACTTCACT
PRIMER_RIGHT_SEQUENCE=GGTGTGAAAGGGTTTGATGATT
PRIMER_LEFT=228,22
PRIMER_RIGHT=506,22
PRIMER_LEFT_TM=60.146
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.2761
PRIMER_LEFT_1_PENALTY=0.145769
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=TCTTCTTCCCCACAACTTCACT
PRIMER_RIGHT_1_SEQUENCE=AGTGGGTCGGTATGGTTATGAG
PRIMER_LEFT_1=228,22
PRIMER_RIGHT_1=444,22
PRIMER_LEFT_1_TM=60.146
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.3663
PRIMER_LEFT_2_PENALTY=0.270774
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=TCTTCTTCTTCCCCACAACTTC
PRIMER_RIGHT_2_SEQUENCE=GGTGTGAAAGGGTTTGATGATT
PRIMER_LEFT_2=225,22
PRIMER_RIGHT_2=506,22
PRIMER_LEFT_2_TM=59.729
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.3668
PRIMER_LEFT_3_PENALTY=0.145769
PRIMER_RIGHT_3_PENALTY=0.220992
PRIMER_LEFT_3_SEQUENCE=TCTTCTTCCCCACAACTTCACT
PRIMER_RIGHT_3_SEQUENCE=AAAGGGTTTGATGATTCTGGTG
PRIMER_LEFT_3=228,22
PRIMER_RIGHT_3=500,22
PRIMER_LEFT_3_TM=60.146
PRIMER_RIGHT_3_TM=60.221
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.3727
PRIMER_LEFT_4_PENALTY=0.145769
PRIMER_RIGHT_4_PENALTY=0.226971
PRIMER_LEFT_4_SEQUENCE=TCTTCTTCCCCACAACTTCACT
PRIMER_RIGHT_4_SEQUENCE=AAGTGATGGTGTGAAAGGGTTT
PRIMER_LEFT_4=228,22
PRIMER_RIGHT_4=513,22
PRIMER_LEFT_4_TM=60.146
PRIMER_RIGHT_4_TM=59.773
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_01947 Ps_contig_mira-and-tgicl-ass_franssen_17536
SEQUENCE=ATACCCCAACGCCTGCTTCACTTCCAGCAAGTGAAGTTCCATCCACGCGGTGTCGGTGTTTCCGAGAGGCGCTTGAGGTAGACATGAACTCAAAACGGGGAATGAGTTTAGGGTTAGTTGAGGACTGTTGAGGATTCTCTTTGCGAGTTTTTTGTTTTGTAGCGGTGGTTTTAGGATTGAGATTCTTGAAGGTGTTGTTTTGGTCGGTGAATTTTGGTTTTTTGGTTTTGTATTGATCGAGTTCTTTGAGGAGGCGTTGAGGGCTGTTTGGAGGAAGTTGTTGGGGGTGAGTTTTATGGKTGTTGCGGTAATGCGGGTAGGGTTTTTGGGTGTGTTGGTTTGGGGTTTTGGGGTTTTGGTGGTGAATTGGATTGGATTGGGAGAGATTGAGGNTGAGGATGAGGATGAGAGTGAGTGGTTGGGTTTAGGGAATGG
TARGET=366,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01948 Ps_contig_mira-and-tgicl-ass_franssen_17554
SEQUENCE=GARACACTGGTTGGTGCTGACATACAGGAACACTTATGATGTCTTTGGTGTCGCAAATTGGATTCGCACACTTCACTCAACCTTCATCTCTATCTTCTCCTTCCATCGCCCCTTTCCGCCACAACCCTTGTTCTTCAATGTCTTCTTCTTCTTCGTGGACACCATATGAGGACAAGGATGGTAAAAAATACAAAGAAAGTGTGGATATGGACACAGAACAAGCAGGTTATGACTGCAGCTGTTGAGAGAGGATGGAACACTTTCATTTTCCCATCAAACCTTCCTCAACTCGCCAATGATTGGTCATCCATTGCAGTAATCTGTCCTCTTTTTCTTGGTGAGGGAGAGATTTTGGATGCACAGAATAAAAGGGTAGCTACGGTTTTTGACGTCTCGACGCCGGAGGAATTAGAGGGACTTCGACCCGAGGATGAGCATGCAGAGAACATTGTAAG
TARGET=141,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01949 Ps_contig_mira-and-tgicl-ass_franssen_17569
SEQUENCE=GACCATGGTCTGGTAGAGTAGCAGAGTCACAATTTCAATGGCCACAGAAATTTGTGGCGGGTCTCTATTCTTATCCGTTAAAACTGAATTGCAACGCCAAAAGCTTTTACTCCATTCTCAACGAACAACAACAACCGCCACTAGATTCCACTCATCCAAACCTCGCTTTTCTTTCCGCTCTTCCAATGCCTCCTCCTTTCACCACCGCAACTCCTCCGTCTTCTGCCGCGTTGCCACCACCGATACACTCTCCGACTCCGACTCTGACTCCACCGCTAGAGACGGCCAGGATCGTCTCTCTAAGGTGCCGGTGTCTAATATAAGAAACTTCTCCATCATTGCTCATATTGACCATGGGAAATCCACGTTAGCCGATAAATTGCTTCAGGTTACTGGTACCGTTCCTCAGCGAGAGATGAAGGAACAGTTTCTTGATAACATGGATTTGGAGAGGGAAAGAGGCATCACTATTAAGCTTCAGGCAGCTCGAATGCGCTATGTGTTTGAAAATAAACCATATTGCTTAAATTTAATAGATACTCCAGGACATGTTGACTTCTCGTACGAGGTTTCTCGATCACTTGCTGCATGTGAGGGTGCTCTCCTTGTAGTAGATGCTTCTCAGGGGGTTGAAGCGCAAACACTCGCTAATGTTTATTTAGCCTTGGCTAACAACTTAGAAATTATCCCTGTTTTGAACAAAATAGATCTTCCGGGTGCCGAACCAGATCGAGTTATCAAGGAGATTGAAGAGGTTATAGGTCTAGATTGTAGCAACGCCATTCTCTGCTCTGCAAAGGAAGGAATAGGTATAATGGAGATTCTCAATGAAATTGTTGCAAAAGTTCCCCCACCCGCTGATACATCAAAAAGGCCATTGAGGGCTTTAATATTTGATAGTTACTATGATCCATATAGAGGTGTTATTGTATACTTTCGAGTTGTAGATGGCACTATAAAGAAAGGTGACAGAGTTACTTTTATTGGCCAGTGGTAAGGGATTATTTTGTCCGATGAAATTGGGGTTTTGGTTCCCCCAACTCAACTTCAAGCTGAAGAGTTGTTTGTCTGGTGGAGGGTGGGCTATCT
TARGET=124,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2455
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=0.232060
PRIMER_LEFT_SEQUENCE=TGTGGCGGGTCTCTATTCTTAT
PRIMER_RIGHT_SEQUENCE=AGTCGGAGTCGGAGAGTGTATC
PRIMER_LEFT=52,22
PRIMER_RIGHT=262,22
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=59.768
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=5.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=0.2455
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=0.232060
PRIMER_LEFT_1_SEQUENCE=TGTGGCGGGTCTCTATTCTTAT
PRIMER_RIGHT_1_SEQUENCE=GAGTCGGAGTCGGAGAGTGTAT
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=59.768
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.2735
PRIMER_LEFT_2_PENALTY=0.013401
PRIMER_RIGHT_2_PENALTY=0.260060
PRIMER_LEFT_2_SEQUENCE=TGTGGCGGGTCTCTATTCTTAT
PRIMER_RIGHT_2_SEQUENCE=CACCTTAGAGAGACGATCCTGG
PRIMER_LEFT_2=52,22
PRIMER_RIGHT_2=306,22
PRIMER_LEFT_2_TM=59.987
PRIMER_RIGHT_2_TM=60.260
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=0.3391
PRIMER_LEFT_3_PENALTY=0.013401
PRIMER_RIGHT_3_PENALTY=0.325733
PRIMER_LEFT_3_SEQUENCE=TGTGGCGGGTCTCTATTCTTAT
PRIMER_RIGHT_3_SEQUENCE=ATTAGACACCGGCACCTTAGAG
PRIMER_LEFT_3=52,22
PRIMER_RIGHT_3=318,22
PRIMER_LEFT_3_TM=59.987
PRIMER_RIGHT_3_TM=59.674
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.4164
PRIMER_LEFT_4_PENALTY=0.013401
PRIMER_RIGHT_4_PENALTY=0.402961
PRIMER_LEFT_4_SEQUENCE=TGTGGCGGGTCTCTATTCTTAT
PRIMER_RIGHT_4_SEQUENCE=GCACCTTAGAGAGACGATCCTG
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=307,22
PRIMER_LEFT_4_TM=59.987
PRIMER_RIGHT_4_TM=60.403
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_01950 Ps_contig_mira-and-tgicl-ass_franssen_17574
SEQUENCE=AAAGCGAGGAGGAAGGTGAAGATGAGGACGTATGATGATGAAGATCCTCTTTTAGGCCCTGGCTTTGGCGGGGAAAGCGACAGTGATGCTGATGACATGAGCAATGATGATGATAGTGTAAGTGATCCCGATGATGATGATGATGAAGATGGAGGTTTCATAATGAATGATGTAGAACTTTAGAATAGGAGAAGCAGACATGTTAGATATGGTATCGGATGGTGACGAGGAAGATGATGACAGTCAAGGACTTTCAGAGAGTAGTGACGATTATGACTTTGGTTATTAATTATTTGTCTGAGAGGCTGTAATTATATTCTCTTCATTGTATTCTATTTTTAGGGACAAAAGGTTCGTTTTTATTTTTCACTAATTTTGCTGTTA
TARGET=130,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3170
PRIMER_LEFT_PENALTY=0.062402
PRIMER_RIGHT_PENALTY=0.254613
PRIMER_LEFT_SEQUENCE=ATGAAGATCCTCTTTTAGGCCC
PRIMER_RIGHT_SEQUENCE=TTGACTGTCATCATCTTCCTCG
PRIMER_LEFT=37,22
PRIMER_RIGHT=246,22
PRIMER_LEFT_TM=59.938
PRIMER_RIGHT_TM=60.255
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=12.4000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.3351
PRIMER_LEFT_1_PENALTY=0.062402
PRIMER_RIGHT_1_PENALTY=0.272667
PRIMER_LEFT_1_SEQUENCE=ATGAAGATCCTCTTTTAGGCCC
PRIMER_RIGHT_1_SEQUENCE=ACTGTCATCATCTTCCTCGTCA
PRIMER_LEFT_1=37,22
PRIMER_RIGHT_1=243,22
PRIMER_LEFT_1_TM=59.938
PRIMER_RIGHT_1_TM=59.727
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=12.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.3351
PRIMER_LEFT_2_PENALTY=0.062402
PRIMER_RIGHT_2_PENALTY=0.272667
PRIMER_LEFT_2_SEQUENCE=ATGAAGATCCTCTTTTAGGCCC
PRIMER_RIGHT_2_SEQUENCE=CTGTCATCATCTTCCTCGTCAC
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=242,22
PRIMER_LEFT_2_TM=59.938
PRIMER_RIGHT_2_TM=59.727
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.3351
PRIMER_LEFT_3_PENALTY=0.062402
PRIMER_RIGHT_3_PENALTY=0.272667
PRIMER_LEFT_3_SEQUENCE=ATGAAGATCCTCTTTTAGGCCC
PRIMER_RIGHT_3_SEQUENCE=TGACTGTCATCATCTTCCTCGT
PRIMER_LEFT_3=37,22
PRIMER_RIGHT_3=245,22
PRIMER_LEFT_3_TM=59.938
PRIMER_RIGHT_3_TM=59.727
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=12.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.8063
PRIMER_LEFT_4_PENALTY=0.551677
PRIMER_RIGHT_4_PENALTY=0.254613
PRIMER_LEFT_4_SEQUENCE=GAAGATCCTCTTTTAGGCCCTG
PRIMER_RIGHT_4_SEQUENCE=TTGACTGTCATCATCTTCCTCG
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=60.552
PRIMER_RIGHT_4_TM=60.255
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_01951 Ps_contig_mira-and-tgicl-ass_franssen_17585
SEQUENCE=TTTCCATGGCATCCTCCTAAATTGTTCATCCAATATATTCAGCATCAATTTTTATCACCCTTTTTAGTGGGAACACTCTTCATTCATTCAAAACCAATCCGAAAATGGAAGAGCACGAAAATGGCGCGAATGACAAACGAAGGCGAACCCATAGTTGGACCCGGCCCAGCTCCTCGTGCTCGACTCAAGCGACCTCTCCAGTTCGAACAAGCTTATCTCGATGCTTTACCTTCCGCTAATATGTATGAGAAAAGCTATATGCATCGTGATGTGGTTACACATGTTGCTGTTTCGGCTGCAGAGTTTTTCATTACTGGAAGTGCTGATGGGCATTTGAAGTTTTGGAAGAAAAGGCCTATTGGTATTGAGTTTGCGAAACACTTCAGATCTCATCTTGGTCCTATTGAAGGTCTAGCTGTTAGTATTGATGGTCTGCTTTGCTGTAA
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3988
PRIMER_LEFT_PENALTY=0.203971
PRIMER_RIGHT_PENALTY=0.194782
PRIMER_LEFT_SEQUENCE=CATGGCATCCTCCTAAATTGTT
PRIMER_RIGHT_SEQUENCE=CAACATGTGTAACCACATCACG
PRIMER_LEFT=4,22
PRIMER_RIGHT=285,22
PRIMER_LEFT_TM=60.204
PRIMER_RIGHT_TM=59.805
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.4537
PRIMER_LEFT_1_PENALTY=0.258908
PRIMER_RIGHT_1_PENALTY=0.194782
PRIMER_LEFT_1_SEQUENCE=ATCACCCTTTTTAGTGGGAACA
PRIMER_RIGHT_1_SEQUENCE=CAACATGTGTAACCACATCACG
PRIMER_LEFT_1=53,22
PRIMER_RIGHT_1=285,22
PRIMER_LEFT_1_TM=59.741
PRIMER_RIGHT_1_TM=59.805
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.6651
PRIMER_LEFT_2_PENALTY=0.203971
PRIMER_RIGHT_2_PENALTY=0.461118
PRIMER_LEFT_2_SEQUENCE=CATGGCATCCTCCTAAATTGTT
PRIMER_RIGHT_2_SEQUENCE=CGAAACAGCAACATGTGTAACC
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=60.204
PRIMER_RIGHT_2_TM=60.461
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.6651
PRIMER_LEFT_3_PENALTY=0.203971
PRIMER_RIGHT_3_PENALTY=0.461118
PRIMER_LEFT_3_SEQUENCE=CATGGCATCCTCCTAAATTGTT
PRIMER_RIGHT_3_SEQUENCE=CCGAAACAGCAACATGTGTAAC
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=60.204
PRIMER_RIGHT_3_TM=60.461
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.7156
PRIMER_LEFT_4_PENALTY=0.254467
PRIMER_RIGHT_4_PENALTY=0.461118
PRIMER_LEFT_4_SEQUENCE=TCACCCTTTTTAGTGGGAACAC
PRIMER_RIGHT_4_SEQUENCE=CCGAAACAGCAACATGTGTAAC
PRIMER_LEFT_4=54,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=60.254
PRIMER_RIGHT_4_TM=60.461
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_01952 Ps_contig_mira-and-tgicl-ass_franssen_17595
SEQUENCE=TATCAACGCAGAGTAGTCGGGGACTGCCTCTGTCTTCAATCCTTCGTTTCTCGATCTCTTGGCTAGTCTCTTCTTCTTCTTCTTCGCAACTTCACCAAACCTCAAGATGCAGAACGAAGAAGGACAAATCACCGAGCTCTACATTCCTAGGAAGTGTTCTGCTACAAACAGATTGATTACTGCTAAGGATCATGCTTCCGTTCAGATTAACGTAGGTCATTTGGATGAGAGTGGTGTCTACAATGGTCACTTCTCTACCTTCGCTCTATGCGGCTTCACTCGCGCTCAGGGAGATGCTGACAGTGGAATTGACCGCTTGTGGGCGAAAAAGAAAACTGAACTTAAGCAATAGATGACATGATTACATCTTTGCTGTGTTTCTTTTTGACAGATTTTATGTGTTGAGTTCAACCTTTCCCTAGATATTGGATTATTTTGATCTTTGTATTACGATACTTGTTTTAAATTTCCTTTGGCACCTTCAATTTCAATGCATTCACCTAAATTGCTATCCATTCCTTTCTTTCATCTTTCTTCATCCTTGATGTTTCTTCTTAGTTATTGAAAATATGTTATGCAATGAGTAGTGCAACCGTTTTCTAATTTTGAAAGCCATGTTCGAGACT
TARGET=69,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1321
PRIMER_LEFT_PENALTY=0.115189
PRIMER_RIGHT_PENALTY=0.016950
PRIMER_LEFT_SEQUENCE=GACTGCCTCTGTCTTCAATCCT
PRIMER_RIGHT_SEQUENCE=GGTGATTTGTCCTTCTTCGTTC
PRIMER_LEFT=21,22
PRIMER_RIGHT=132,22
PRIMER_LEFT_TM=59.885
PRIMER_RIGHT_TM=59.983
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=112
PRIMER_PAIR_PENALTY_1=0.1429
PRIMER_LEFT_1_PENALTY=0.115189
PRIMER_RIGHT_1_PENALTY=0.027715
PRIMER_LEFT_1_SEQUENCE=GACTGCCTCTGTCTTCAATCCT
PRIMER_RIGHT_1_SEQUENCE=ACGTTAATCTGAACGGAAGCAT
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=212,22
PRIMER_LEFT_1_TM=59.885
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.2234
PRIMER_LEFT_2_PENALTY=0.115189
PRIMER_RIGHT_2_PENALTY=0.108206
PRIMER_LEFT_2_SEQUENCE=GACTGCCTCTGTCTTCAATCCT
PRIMER_RIGHT_2_SEQUENCE=GTCCTTCTTCGTTCTGCATCTT
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=124,22
PRIMER_LEFT_2_TM=59.885
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=0.2528
PRIMER_LEFT_3_PENALTY=0.115189
PRIMER_RIGHT_3_PENALTY=0.137575
PRIMER_LEFT_3_SEQUENCE=GACTGCCTCTGTCTTCAATCCT
PRIMER_RIGHT_3_SEQUENCE=ACCACTCTCATCCAAATGACCT
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=234,22
PRIMER_LEFT_3_TM=59.885
PRIMER_RIGHT_3_TM=59.862
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.2585
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=0.016950
PRIMER_LEFT_4_SEQUENCE=GTCTTCAATCCTTCGTTTCTCG
PRIMER_RIGHT_4_SEQUENCE=GGTGATTTGTCCTTCTTCGTTC
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=132,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=59.983
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=102
=
PRIMER_SEQUENCE_ID=SSR_01953 Ps_contig_mira-and-tgicl-ass_franssen_17608
SEQUENCE=GAACACCTCGCCGTTTGATGTATAAAACGCAACACAAATACCAGCTGTATTATTCGATGGAAGTTTTATATTAGCACTGAAGAATCCATATTGGTACATATTGGATGAAATAAAACCAGAACCGGTGTAACGATCAAGGAGGAGTTGAACGCCGTTACCGTCGGCGGAGCGAACAACGTTGCCATCTCCGAAGAGATGAGAGTAACCGTCGTTGAAGGGAATGGTGGCAAGGTCGAAAGCTGAGGTTGTGGAAGAGAAGAGAAGAAAAAGAGAGAAAAAGAATAGAGAAAGAGTTTTAGGTAGTGAAGTTTGAAAAGAACGTAAGGGATGGTGATCCATAATAATTTAAAAAATAAAAATAAAAATAGATTATGATGTGTTGATTCTCTCTCTCTTACACTTGTTTCAMMCGCGTACTCTGCGTTGA
TARGET=251,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01954 Ps_contig_mira-and-tgicl-ass_franssen_17608
SEQUENCE=GAACACCTCGCCGTTTGATGTATAAAACGCAACACAAATACCAGCTGTATTATTCGATGGAAGTTTTATATTAGCACTGAAGAATCCATATTGGTACATATTGGATGAAATAAAACCAGAACCGGTGTAACGATCAAGGAGGAGTTGAACGCCGTTACCGTCGGCGGAGCGAACAACGTTGCCATCTCCGAAGAGATGAGAGTAACCGTCGTTGAAGGGAATGGTGGCAAGGTCGAAAGCTGAGGTTGTGGAAGAGAAGAGAAGAAAAAGAGAGAAAAAGAATAGAGAAAGAGTTTTAGGTAGTGAAGTTTGAAAAGAACGTAAGGGATGGTGATCCATAATAATTTAAAAAATAAAAATAAAAATAGATTATGATGTGTTGATTCTCTCTCTCTTACACTTGTTTCAMMCGCGTACTCTGCGTTGA
TARGET=385,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01955 Ps_contig_mira-and-tgicl-ass_franssen_17620
SEQUENCE=ATCAACGCAGAGTACTCGGGGGATACACACCCATTTGATTTGAGTGAGAAAAGAAAAATGGGTAAGAACAAGAAAAAGAGAACTAGGGTTTCCGTTTCCGATGACGATGAAGAAATCGAATCCGATAACAACAACAACGATTCCAATCAGAAAGATAATTCCCTCTACCAGGTTCTTGGTGTGGACAGTACAGCGACTCAACAGGAAATAAAGAAGGCGTATCACAAATTGGCGTTGAGGCTTCATCCTGATAAGAACCCTGGCGACGAGGAAGCTAAAGCCAAGTTTCAGCAATTGCAAAGTGTTATCTCAATTCTTGGAGATGAACAAAAACGGGCAATGTATGATCAGACTGGTTCTGTTGAACACGCCGACCTTGCTGGAGATGTTGTTCAGAATCTTCACGAATATTTCAGAACAATGTACAAGAAGATCACTGAAGCTGATATTGAGGAGTTCGAAGCAAACTATAGAGGATCTGACTCTGAGAAAACTGATTTGATTAATCTCTACAAGGAATGCAAAGGAAATATGAACAGGTTATTCTGTTCAATGCTTTGTTCAGATCCTAAACTCGACTCACACCGATTCAAGGATATTATTGATGAGGCCATAGCTTCTGGAGAACTAAAGGAAAAGAAAGCTTACAAGAAATGGGCAAAGAAAATATCAGAAACCAAGCCACCAACTAGTCCTTTAAGAAGCAGGGCAAAAGCAAATAAGGATCCAAAAACGGATTTGTATGCTATCATATCAAAACGACAAAATGAGAGGAAAGGCCAGCTTGATTCTATGTTCTCGTCTTTAATTTCAAAGTATGGGGGAGAGCATGCGCCAGAACCAACTGAAGAGGAATTTGAAGCTACACGAAGAAAGATGGAAAGCCGAAGATCCTCAAAAAAGGCAAAGCGGAAATAACCAACTTACCTTTGTGAATAGTCTGTTTCTCTCATGGTGACTGTCACCTCAGCATTGTTGGTAGGAACGACAGGGTTATTTTCATATGCAAGTTTATATAACTGGTTAGTTAATGTTTAAGTAGACCAGTAACATTTAAAAAATGAGGCATGTGTCTTCAATGTTCTTGGTGAATTTATGCTTTAAAAGCAGCCGAACTATTTTATGGTACCATGGCATTTAAAAYTTTTATCACAAGACACACACACATACATAGTACTGCAAATT
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01956 Ps_contig_mira-and-tgicl-ass_franssen_17620
SEQUENCE=ATCAACGCAGAGTACTCGGGGGATACACACCCATTTGATTTGAGTGAGAAAAGAAAAATGGGTAAGAACAAGAAAAAGAGAACTAGGGTTTCCGTTTCCGATGACGATGAAGAAATCGAATCCGATAACAACAACAACGATTCCAATCAGAAAGATAATTCCCTCTACCAGGTTCTTGGTGTGGACAGTACAGCGACTCAACAGGAAATAAAGAAGGCGTATCACAAATTGGCGTTGAGGCTTCATCCTGATAAGAACCCTGGCGACGAGGAAGCTAAAGCCAAGTTTCAGCAATTGCAAAGTGTTATCTCAATTCTTGGAGATGAACAAAAACGGGCAATGTATGATCAGACTGGTTCTGTTGAACACGCCGACCTTGCTGGAGATGTTGTTCAGAATCTTCACGAATATTTCAGAACAATGTACAAGAAGATCACTGAAGCTGATATTGAGGAGTTCGAAGCAAACTATAGAGGATCTGACTCTGAGAAAACTGATTTGATTAATCTCTACAAGGAATGCAAAGGAAATATGAACAGGTTATTCTGTTCAATGCTTTGTTCAGATCCTAAACTCGACTCACACCGATTCAAGGATATTATTGATGAGGCCATAGCTTCTGGAGAACTAAAGGAAAAGAAAGCTTACAAGAAATGGGCAAAGAAAATATCAGAAACCAAGCCACCAACTAGTCCTTTAAGAAGCAGGGCAAAAGCAAATAAGGATCCAAAAACGGATTTGTATGCTATCATATCAAAACGACAAAATGAGAGGAAAGGCCAGCTTGATTCTATGTTCTCGTCTTTAATTTCAAAGTATGGGGGAGAGCATGCGCCAGAACCAACTGAAGAGGAATTTGAAGCTACACGAAGAAAGATGGAAAGCCGAAGATCCTCAAAAAAGGCAAAGCGGAAATAACCAACTTACCTTTGTGAATAGTCTGTTTCTCTCATGGTGACTGTCACCTCAGCATTGTTGGTAGGAACGACAGGGTTATTTTCATATGCAAGTTTATATAACTGGTTAGTTAATGTTTAAGTAGACCAGTAACATTTAAAAAATGAGGCATGTGTCTTCAATGTTCTTGGTGAATTTATGCTTTAAAAGCAGCCGAACTATTTTATGGTACCATGGCATTTAAAAYTTTTATCACAAGACACACACACATACATAGTACTGCAAATT
TARGET=1157,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01957 Ps_contig_mira-and-tgicl-ass_franssen_17621
SEQUENCE=CTGAAACTTGGCTTTAGCTTCCTCGTCGCCAGGGTTCTTATCAGGATGAAGCCTCAACGCCAATTTGTGATACGCCTTCTTTATTTCCTGTTGAGTCACTGTACTGTCCACACCAAGAACCTGGTAGAGGGAATTATCTTTCTGATTGGAATCGTTGTTGTTGTTATCGGATTCGATTTCTTCATCGTCATCGGAAACCCTAGTTCTCTTTTTCTTGTTCTTACCCATTTTTCTTTTCTCACTCMAATCC
TARGET=154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01958 Ps_contig_mira-and-tgicl-ass_franssen_17634
SEQUENCE=GTCGGGGACACCCAGACACATACATAAATGCTGAAGAAGAAAAAAAGAGTAAGAACTCTTTCTCTTTCTCTATTTCCTTCCCATTCTCTTTCCTTTACCTCAAAAAAGACAAAACAAAATGCAGTTTATGTTAAACCTTCGTGTTACCCACGGTGTTCCTCTCTGTCTCTCCGGAATCACCAACCCTCTTGCTCTTTCCAAAGAGATGGCAGATCACGATCGTAGAAGAAAAGCCATGAGAAAGCAAAGATCAGTACATGCAAATCAAGATAAGGGTTCTCCAAGGAACAGTGATTTTGTTGAGATCAAAGGTGGTGAATCTGGTGAAGGTTATGATCAGGAAGATCTGGAGGAAATCTTGTATCAATTGTCTCGTAGTTATTATCTTACAGGTTTATCAGCAAAAGCTGCTTAATTAAAACTACATTAATTAATCTCTTTCATTTTCTGAACTATGTTGTTCTTGTTGTTGTTGTAGATTTTCTTTTACTGTGTTGTTGTTGTAACATGTAAAAAAAACATCTTCTGATTCAGTGTTGTTCCGTTTTGTTATTTGATATATATCTTTAAATTATTGTGTATGTTTCACCAACAATATAAGGTTAAGTTTTGGAASGTAAC
TARGET=464,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01959 Ps_contig_mira-and-tgicl-ass_franssen_17634
SEQUENCE=GTCGGGGACACCCAGACACATACATAAATGCTGAAGAAGAAAAAAAGAGTAAGAACTCTTTCTCTTTCTCTATTTCCTTCCCATTCTCTTTCCTTTACCTCAAAAAAGACAAAACAAAATGCAGTTTATGTTAAACCTTCGTGTTACCCACGGTGTTCCTCTCTGTCTCTCCGGAATCACCAACCCTCTTGCTCTTTCCAAAGAGATGGCAGATCACGATCGTAGAAGAAAAGCCATGAGAAAGCAAAGATCAGTACATGCAAATCAAGATAAGGGTTCTCCAAGGAACAGTGATTTTGTTGAGATCAAAGGTGGTGAATCTGGTGAAGGTTATGATCAGGAAGATCTGGAGGAAATCTTGTATCAATTGTCTCGTAGTTATTATCTTACAGGTTTATCAGCAAAAGCTGCTTAATTAAAACTACATTAATTAATCTCTTTCATTTTCTGAACTATGTTGTTCTTGTTGTTGTTGTAGATTTTCTTTTACTGTGTTGTTGTTGTAACATGTAAAAAAAACATCTTCTGATTCAGTGTTGTTCCGTTTTGTTATTTGATATATATCTTTAAATTATTGTGTATGTTTCACCAACAATATAAGGTTAAGTTTTGGAASGTAAC
TARGET=493,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01960 Ps_contig_mira-and-tgicl-ass_franssen_17638
SEQUENCE=CGGACGGGGGTTAGGCAAGTAACTATCCCGAGTTGTAAGCCTTTTCGTACTCAGTGAGCGAAGACCAAAAAAGGGTTTAAACTCAGTCAACCGAGTTGATGAATCTCCTCGCAATAACCTCCACTTCTATCTTCTTCCTCCCCGTTTCCTTCTTTCATCCCTTGTTTGCAGCAAGAGCATCTGCGTTTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCATACAAGTTCACATCCAGCGCAGGTTTAAGAAGATGCGTCAACAAGGAAAAGGAAGAGGTGCTTCTAGAAGGGATGCCTTCTCACTATTATGATGATGAATGGCAAGCCCAACAACGTGAAAATACCAAGGAGTTGCATCGAAGACGCAGAGAAGAAGACGAGGAAGAAGAGAGAAAGATTGACGAGTATCGTGAAGTTGGCATGCGGCTGAAGGGATACCCTGAAGAAGATGTTAGAAATGCAYGGAAATTGGTTTCAAGCTTCATAAGAGCTGTTGAAGAAGTAGAAGAGAAAATTGAGGAAGCTGCTGAGAAGGGAGAACTTACCGAACTTGTTTTGTTGGTCATATGGAATCGTCTTGATCTTGCCCGGCGTGATGATGAAAAGGATGCTATAAGAAGTCTGGATCTGTTGTACAGAAGGGTTGAGACTGAGATCTTGAAGCGAGAGGCTACCCCTGCAATGAGATTGCTCAATGATCTTTTGATTATGTATGATGGCTTTAATTTCGACGAGTGGTTAAATAAATGTAAAAAGATCATGAGTGATACATTTCCACAGGAGGATCCATATAGCATTCTTGTTCCACCTGGATTTGAGTCATTTGACATAGATAAGCATCACGGGCCATGGCGACCATCACTTGAAATCGATGATAATACTCTTTTGAGGGTGGACTTTGTAAGGGAGGTGGATGAACTGCTGCAAGAGGTTCGTTCGGAACAGGATGAAGAAGAAAATGAACAAGAGTTTGATCCTGAATCAGTTGCAAATAGATTAAAGCAACAAGAGAAGCAACAAACTATACGTCAAGTAGAAGCTCTGCTAGATTTAGCCATTAGTTTGAAATGGTAGCTATTTGGTCCAAGCTTGTATTATGATTTACGTATATCTTTTCAGATGTCCTACAGTTTATAGTTTTGAATAGAAATTAGTTTGTAGTGCAATTTCAATCCAAGTAATTTCATGGTGTTGTTAATTTTTCCTTATAAGATAA
TARGET=190,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01961 Ps_contig_mira-and-tgicl-ass_franssen_17640
SEQUENCE=CTTGAAAATCACTCAAAATACCAATGAGTTCTCCAATGAAAGTAGAAGCTCACAGTAATCCATTTCTCATAGGTGCAATAGTGACCTAATCGGTGTTAGTTCAACAAAACAACAACAACCGAACCTTATCTCACTAAAGTGAAGTCAGCTATATGGATCAGCTTTCCATACTTCTATCAAAATCGTTAATCTTGAGAACTTTCTTAATAACTTTTCTTATAATTATCTAGGTCTTCCTCTATCTCTAATTGTTTGACTTCTCTTCATCTGATCTACTCTTCTTACTACATAATCTACGAATCTTCTCTCTACATGCTCAATCCACCTAGCCTATTTTCCTACCCATCCATAGGTTGTAG
TARGET=108,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0861
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=0.068302
PRIMER_LEFT_SEQUENCE=TGCAATAGTGACCTAATCGGTG
PRIMER_RIGHT_SEQUENCE=CAACCTATGGATGGGTAGGAAA
PRIMER_LEFT=73,22
PRIMER_RIGHT=355,22
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=60.068
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.1336
PRIMER_LEFT_1_PENALTY=0.017846
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=TGCAATAGTGACCTAATCGGTG
PRIMER_RIGHT_1_SEQUENCE=AATAGGCTAGGTGGATTGAGCA
PRIMER_LEFT_1=73,22
PRIMER_RIGHT_1=334,22
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.4124
PRIMER_LEFT_2_PENALTY=0.017846
PRIMER_RIGHT_2_PENALTY=0.394536
PRIMER_LEFT_2_SEQUENCE=TGCAATAGTGACCTAATCGGTG
PRIMER_RIGHT_2_SEQUENCE=ATGGATGGGTAGGAAAATAGGC
PRIMER_LEFT_2=73,22
PRIMER_RIGHT_2=349,22
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=60.395
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.4247
PRIMER_LEFT_3_PENALTY=0.017846
PRIMER_RIGHT_3_PENALTY=0.406861
PRIMER_LEFT_3_SEQUENCE=TGCAATAGTGACCTAATCGGTG
PRIMER_RIGHT_3_SEQUENCE=ACAACCTATGGATGGGTAGGAA
PRIMER_LEFT_3=73,22
PRIMER_RIGHT_3=356,22
PRIMER_LEFT_3_TM=60.018
PRIMER_RIGHT_3_TM=59.593
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.8089
PRIMER_LEFT_4_PENALTY=0.740565
PRIMER_RIGHT_4_PENALTY=0.068302
PRIMER_LEFT_4_SEQUENCE=GGTGCAATAGTGACCTAATCGG
PRIMER_RIGHT_4_SEQUENCE=CAACCTATGGATGGGTAGGAAA
PRIMER_LEFT_4=71,22
PRIMER_RIGHT_4=355,22
PRIMER_LEFT_4_TM=60.741
PRIMER_RIGHT_4_TM=60.068
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.8000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_01962 Ps_contig_mira-and-tgicl-ass_franssen_17670
SEQUENCE=GGYKKKAAGGAATTATAGGACCAACATAAAGAAGAAAAATAGTCATTGAAACTTCAACCGTGAAGATTCAATTCAAAAGCAAATTAACATCACACGGCATAAATTTCAAATGTAAGAGTACAACATATAGAAAATCAAATGATACAAAACAAATAATAATATAATGGTCGAAGACAGACAGCTTCCTTATGCTCTCTGGCTCTAACCATGGCGGGCACTAGTGCTACCACCTGACCATCTTCTCAAAGTACTAGCCACTATTTTCCTCCCAGATAAAAGTCCTAACCTTTGCTTGAACGAAGCCGATCGAACAGTACTTCTTGATGCAATATTTCCCGATGTAGATGCTGCTGCGCAGACAGGCATTGGAGGAAACTCTATAGCATTGTCTTGTGCTCCACCATCACTAGCACTAGCATCTTGAAGTTCCGCTTCGACAGTATCCAAATTAACAGCAACTGATCCCCGAGTGAGCCTCAACCAATCTCCACCCTCAGGGGTAACGCCTTTACATTTCTTAACCTTCACTTTCACCAAATTGGGACACCCATTCGCCAAAGCTTCCATTCCATGATCAGAAACAGGACAGCTCTTAATGCAGAGTTTCTTTAAAGCTAAACACTTTGCAGCAATGCATGATATCTCCGGATCACCAACAGTATCACTTGCACACAAAGCCAACCTCTCTAGATTAGGGCAATTAGATGCCAACATTTCCAAACTTGCTTTTGTAGGGTTAACACCAATAAGCACCAACTCTTGTAGATTAGGGCAAAACTTAGCAACCGCTATTAACCCTTCGTCACCGATCCGATTCGCTTTCCATCCATCAATGTGAAGCTTCCTTAACAACTTACACCGCTCCGCAATCGCAACAAGTCCCAAATCAGTGCACTCAGGTGTCTTAACAAGATGCAAAATCTCCAAATTCGAACAATGTGAAATAGCTTGTAATCCAATATCACTAATCTGAAGCCTCTCAAAGTGAACATCAGCAATCATGCTCGTGACACGCTCCGCCATAAGCGTAAAAAGCGTGTCCCAATCACCCGAGCATCGAAAAAGCTTCAAGGTTTTCAGATTCTTCGCACCAAGAATCAACGAACCAAAACACTGTCCATTATAAAGCTCCTTCAAACAAATCGTCTTGAGCGACGCCGCCGCTACACCCGGCCCAATCGGCTCCGCCACCGCAGTCTCCGCAATCCCCCGGAGCCGCTTCACCGACAATTCCTCAAGAGCAGCACAATTATCCAGCACCGCGTTCATACCTTTAGATCCAAAAGTACAAGATCCACACGATAATTTCTTCAATCCTTTACAATTCCTCGCGAAAGTTTCCATTCCAGCATCCGTAAGCTCACGACACGCGCGGAGCTTAAGGCGCGTGAGATTAGGGCAACGCTCGGAGATTATAACGAGAGCTTCATCTCTTATACTCACGGATCTACGATCGCATTTCAAAGCAAGTTTCGTCACCGAATCAAATCGATTGAAGAGAGACGAAATCACCGGAAGCAGATCCAGTTTCGCGTTTAACGAAAGACGCTGCCGACTCTGTCCTTCAACAATCAACCACCGCCGGCAAACGAGTGAACACTGATTCCGATCGGCTGGGCTCAACGACTGAAACACAATCGCTAAACACTCGTCAGGTAGATCTGATATATAATCAGAAATTCCACGGTCAACCGGATGTCTAATATCATCATCATCTTCTTCGGTAGCGTTCATCGGAAGAAACCAAAGAGGTAGATCTGGTGGTTTTCGCGGTGGTTCTTTCCCGGCGGTTGGCGGACGGCGGCG
TARGET=1705,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01963 Ps_contig_mira-and-tgicl-ass_franssen_17716
SEQUENCE=ATCAAGGATTGCTTGAATACCACGATTCTAGCTAGAGAGAATGGAACTGGAGCCACGTTAGCCATTTGAGTAGTCAATTGAGAATTTGGGAATGGCGTAGCTTGAGGCGCTTGCAGAGAATTCTGAAATCCGAAACCGGTAGAAGGTGATTGTTGAAAGAGTGAAGTTTGTTGCTGCTGCTGCTGTGGTTGCTGTTGCTGTTGAGAAGAGAATGGTGAGGAGAAAAGGGAAGAACCACCGAAAGCAGGAGTAGAAGAAGCCCCAAAAGCAGGGGTTGAAGAAGGGGTTCCGAAACCTGGGGTACTGAAACCTGGAGTGGAAGATGGGGTACCGAAATTTGGGGTACCAAATGCCGG
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3331
PRIMER_LEFT_PENALTY=0.137550
PRIMER_RIGHT_PENALTY=0.195574
PRIMER_LEFT_SEQUENCE=AGGATTGCTTGAATACCACGAT
PRIMER_RIGHT_SEQUENCE=GCTTCTTCTACTCCTGCTTTCG
PRIMER_LEFT=4,22
PRIMER_RIGHT=259,22
PRIMER_LEFT_TM=59.862
PRIMER_RIGHT_TM=59.804
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.0000
PRIMER_RIGHT_END_STABILITY=9.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=256
PRIMER_PAIR_PENALTY_1=0.3347
PRIMER_LEFT_1_PENALTY=0.137550
PRIMER_RIGHT_1_PENALTY=0.197140
PRIMER_LEFT_1_SEQUENCE=AGGATTGCTTGAATACCACGAT
PRIMER_RIGHT_1_SEQUENCE=TTCTCTTCTCAACAGCAACAGC
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=59.862
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=0.3471
PRIMER_LEFT_2_PENALTY=0.151533
PRIMER_RIGHT_2_PENALTY=0.195574
PRIMER_LEFT_2_SEQUENCE=TAGAGAGAATGGAACTGGAGCC
PRIMER_RIGHT_2_SEQUENCE=GCTTCTTCTACTCCTGCTTTCG
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=59.848
PRIMER_RIGHT_2_TM=59.804
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=228
PRIMER_PAIR_PENALTY_3=0.3487
PRIMER_LEFT_3_PENALTY=0.151533
PRIMER_RIGHT_3_PENALTY=0.197140
PRIMER_LEFT_3_SEQUENCE=TAGAGAGAATGGAACTGGAGCC
PRIMER_RIGHT_3_SEQUENCE=TTCTCTTCTCAACAGCAACAGC
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=59.848
PRIMER_RIGHT_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.4068
PRIMER_LEFT_4_PENALTY=0.211181
PRIMER_RIGHT_4_PENALTY=0.195574
PRIMER_LEFT_4_SEQUENCE=GGATTGCTTGAATACCACGATT
PRIMER_RIGHT_4_SEQUENCE=GCTTCTTCTACTCCTGCTTTCG
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=259,22
PRIMER_LEFT_4_TM=60.211
PRIMER_RIGHT_4_TM=59.804
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.6000
PRIMER_RIGHT_4_END_STABILITY=9.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_01964 Ps_contig_mira-and-tgicl-ass_franssen_17717
SEQUENCE=GCCATTCATCAAACACCTAGAGKGCAAGCGCGCTTTCTGTTTAGAACTTGAATCACACACACAATTCTCATACYCTCATCTCATCGTCCACACGCCGCACAGCACCATGTTCGGAGCTCAATCTTCTCCTTCTCCCTTCGGAACCCCTTCTACAACACCGGCATTTGGTACCCCAAATTTCGGTACCCCATCTTCCACTCCAGGTTTCAGTACCCCACGGTTTCGGTAACCCCTTCCTTCAA
TARGET=54,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01965 Ps_contig_mira-and-tgicl-ass_franssen_17731
SEQUENCE=AACTTACTATAGTCACAGARRAAAAAACTAAGAAAGGAAAGTAGGGCGTAAAAACAAAGAARGARGGAAACAAAGACGGTGTAAAAGAATAGGGAAATTAGTGGTTGAAGAAGGAAAAAAGAAAGAAAACAAAGTGAATCTGCAGCTGAGAAGGCTGAAGAAAAAGCTAAGGCTGCAAATGACTCTCTGGCTCCGCCGCCGCCGGAGAAAGAGGAAGAGAAACCGGTTGAAACAACTCCGGTGGAAAAAGTGGAACCTCCAAAGGCTTGAAGATCATAAATATGTTGTATATGTGTAATAGTTTGCATATATACAAAAAAATGTTACATACCAAATCTCTTATTCTTTTATATATTGTGTAATTGTAATTTGTCTTTGTCTTTAGTTTGATGCTAGTTGGTAGTAAGTTGGTTGTTAGTTGTATACTTCTTCTTATTTATTTTTAGTTTAACTTTTTTCCTTTTAGTTTTTTTACGTTAACTTTTAAAGTTTTTAACGTTAGTTAAACGTAAAAGGTTAGTTTGTTAGTTACGTTTAAGGTAAAAA
TARGET=193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01966 Ps_contig_mira-and-tgicl-ass_franssen_17732
SEQUENCE=GACCAAAATCAGAATCCCAASGGTCCACTCACAAAAATACTCTCTCTTAACGTGACATGGTTTTGAGAGGGTGAGCAGGAAAGACTCTTTCTCTCACTCCAATCGGTAAAAGGAAAAAACAAGGAATAAAATGAGAGAAGAAAACGAAAGGTTTTCTTCTTCTTCTTCACGGTGACGGGCCTCACCCTGCTCCCTTATTTACATTTTCGGTAGAAACAGTTGTTCCACTCACAAAAAAATATTCTCATCCTCTCCAAAAAAAACGTTCTCTGCAAAACCACCATAACTGTCTTCTTCAACATCTCTGATTCACCTTTGTTCTTCTCTTCCGGCCACCACCAACAACGCCAAAACGCCTCTCCTCTGATCCACAACTTCACTCCGACCAAACAACTCACCTTATCTAAGATATGCAATTTAAACCACCATGTATCCGTTTTTTTTCGGCGAGTTTCTGTACATTCCTCCGTCGGGGCGAGCAAGAACTGTAATAATCAACACAACATCACTAAACGTCATTCCGTAACCAATCGTAAGTTTCTTTGAACATCACCAATACAAGCAAAACATCCACACACCACTTGTTAGCTCCGCCGACAACA
TARGET=154,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01967 Ps_contig_mira-and-tgicl-ass_franssen_17759
SEQUENCE=GAAGAAAAATCAAATCCCATAAACCACAAAGATAAACAAAATTTGAGAACCTCAGAGATACCGTTTTCCTTTTGTAGCAGAAAATGAATTCACCGGCAATGACCTCCCAATCTTCCACCTTCGCCGTCTCCGTTTCTTCTATCGCATCCCCTCTCCGGCGCCGCCGCAACTTCCCCGTCGTACGCGCTCAAGTGGAACCATCTGATAAATCAATTGAAATTATGAGGAAGTTCTCAGAGCAATATGCCCGCAAATCAGGAACATACTTTTGTGTGGACAAAGGAGTAACTTCTGTTGTTATCAAGGGTTTGGCTGACCATAAAGATTCATTGGGTGCACCACTCTGCCCTTGCCGGCATTACGACGATAAAGCTGCCGAGGCTGCACATGGATTTTGGAATTGCCCGTGTGTTCCCATGAGAGAAAGGAAGGAGTGTCATTGCATGCTTTTTCTCACTCCCGATAACGATTTTGCCGGCGATGAACAGACTATCACCTTGGATGAAATTAAAGAATCAACATCAAATATGTAACACAGATTATTGTGTATTAATGTATTTGTGTTAGTTGTTTCAATCTGTAACATTTGAAAAACAAGGGAAGAGTAAAATGTGTAACATTTATATATATATGTAGGTAATATATTTCTACCAAAAGTCACATGTATTTTTTTTCATTTATACTCTTTATAAGTGGCCTAACCCATACTTTGTCAACAAAAAAAAA
TARGET=622,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2364
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.232060
PRIMER_LEFT_SEQUENCE=AAAGGAAGGAGTGTCATTGCAT
PRIMER_RIGHT_SEQUENCE=GACAAAGTATGGGTTAGGCCAC
PRIMER_LEFT=423,22
PRIMER_RIGHT=713,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.768
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.2662
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.232060
PRIMER_LEFT_1_SEQUENCE=TCTCACTCCCGATAACGATTTT
PRIMER_RIGHT_1_SEQUENCE=GACAAAGTATGGGTTAGGCCAC
PRIMER_LEFT_1=451,22
PRIMER_RIGHT_1=713,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.768
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.2662
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.232060
PRIMER_LEFT_2_SEQUENCE=TTCTCACTCCCGATAACGATTT
PRIMER_RIGHT_2_SEQUENCE=GACAAAGTATGGGTTAGGCCAC
PRIMER_LEFT_2=450,22
PRIMER_RIGHT_2=713,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.768
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=264
PRIMER_PAIR_PENALTY_3=0.2662
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.232060
PRIMER_LEFT_3_SEQUENCE=TTTCTCACTCCCGATAACGATT
PRIMER_RIGHT_3_SEQUENCE=GACAAAGTATGGGTTAGGCCAC
PRIMER_LEFT_3=449,22
PRIMER_RIGHT_3=713,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.768
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=265
PRIMER_PAIR_PENALTY_4=0.2662
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.232060
PRIMER_LEFT_4_SEQUENCE=TTTTCTCACTCCCGATAACGAT
PRIMER_RIGHT_4_SEQUENCE=GACAAAGTATGGGTTAGGCCAC
PRIMER_LEFT_4=448,22
PRIMER_RIGHT_4=713,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.768
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_01968 Ps_contig_mira-and-tgicl-ass_franssen_17762
SEQUENCE=AACTTAAAAATATCCAATGGATTGAATAAAATTAGCAAGTATACCATGTAGCCTTTTCAAATAAAGATTATGAAAAGAAGATTTCAATGCTAGTATCATCTGTGAATAAATTCATACTGCAAATAATTACAGGAATTTACTAAATAACACCATGCCTTGCATTCTGAAACTTTTCGTCCAATGATCCTCATCCTCCCTCCCTCCTAGGAAAAATTAGTTACTTTTTCTGCAATCATCAATGCAAATTCTTTGAGCAAGACAGCCTGTCTAATGCAATAAAAATGAATCCCTTCAATGGGCGATTAATTTTGGAAAAAAAATTCCTGTACAGGGAAGGCAAAAACAATCAGACGGTGATTTATCTTCACACGAGGGTGTGTGGTAAATAACAAACCAGTATAACATGATAATGCTTATTTTGATCGCCCCGGTCTTTACTACTGAATCCATTTCTTGGCCTCTTGCTGGATACAATAT
TARGET=192,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2102
PRIMER_LEFT_PENALTY=0.179943
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=GAAACTTTTCGTCCAATGATCC
PRIMER_RIGHT_SEQUENCE=CACACCCTCGTGTGAAGATAAA
PRIMER_LEFT=165,22
PRIMER_RIGHT=378,22
PRIMER_LEFT_TM=59.820
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=214
PRIMER_PAIR_PENALTY_1=0.2140
PRIMER_LEFT_1_PENALTY=0.179943
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=GAAACTTTTCGTCCAATGATCC
PRIMER_RIGHT_1_SEQUENCE=AGGCCAAGAAATGGATTCAGTA
PRIMER_LEFT_1=165,22
PRIMER_RIGHT_1=459,22
PRIMER_LEFT_1_TM=59.820
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=0.3952
PRIMER_LEFT_2_PENALTY=0.179943
PRIMER_RIGHT_2_PENALTY=0.215215
PRIMER_LEFT_2_SEQUENCE=GAAACTTTTCGTCCAATGATCC
PRIMER_RIGHT_2_SEQUENCE=TTATTTACCACACACCCTCGTG
PRIMER_LEFT_2=165,22
PRIMER_RIGHT_2=388,22
PRIMER_LEFT_2_TM=59.820
PRIMER_RIGHT_2_TM=59.785
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.3952
PRIMER_LEFT_3_PENALTY=0.179943
PRIMER_RIGHT_3_PENALTY=0.215215
PRIMER_LEFT_3_SEQUENCE=GAAACTTTTCGTCCAATGATCC
PRIMER_RIGHT_3_SEQUENCE=TGTTATTTACCACACACCCTCG
PRIMER_LEFT_3=165,22
PRIMER_RIGHT_3=390,22
PRIMER_LEFT_3_TM=59.820
PRIMER_RIGHT_3_TM=59.785
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.5239
PRIMER_LEFT_4_PENALTY=0.179943
PRIMER_RIGHT_4_PENALTY=0.343977
PRIMER_LEFT_4_SEQUENCE=GAAACTTTTCGTCCAATGATCC
PRIMER_RIGHT_4_SEQUENCE=GATTGTTTTTGCCTTCCCTGTA
PRIMER_LEFT_4=165,22
PRIMER_RIGHT_4=347,22
PRIMER_LEFT_4_TM=59.820
PRIMER_RIGHT_4_TM=60.344
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_01969 Ps_contig_mira-and-tgicl-ass_franssen_17772
SEQUENCE=ACATGATCTATCAGCAAAGTAGCACAAAACCAATATCTGCTCTTTTCTTTGTCCAGCTTTAGTCAGGAGGGAGAGCTACTTTTGTGATCTTACCATGATGTTCAAACAGCTCCTTCAGACGATCCTGCGTTATGTTTTCTGGAAGGTTCTTTATATATACTGCTTTCACCTGAGAGGATGCTGATGAATCAGAATTCCTTGGGTCAGCCCAGCTCACTGTTGGAGCATTATTGTCAAGTTTAAAATTGGAGTTTGACATTTTCTGCCTTGAATATTCTGCACAAGCATGGTTGTGATACTCAATAAAAGAAAATCCCCGATTACGGCCAGAACTCTGTGGATCCTTCAACAGTTCCACAGCAATGACACCAGGGCCAACCTTGGCAATGACTTTCTTCATATCTTCAACAGTCCAATCTTTAGGAACATTTCCAATGAATAACCTATGCTTAACTTGAGACGTTGAGCACTTTATTTTGTTTCCCTTGAATTCAGTGTTGTTCAACTCCTCGATAGCCTTTGATGCCAATTCCTTTGTCTTGAAGGTCACAAAAGCATAACCTTTTGCTTCTTTTCCCTTCATAATCCTTACCTCTACAACTTCTCCCACAGATCGACAGAAGACCCTTAAATCTTTTTCAGAAATGTCATGAGGAATTCCGCCAATGTAAACCTCTGAACCATGAGGTGGAAGCGCAAGAAGTTCAGCATGTCTCTTTTTCTCAACCTCATCTTTAGTATCTTCCACTCTCATCTCGTCTTCACCATCTGAAGGCTTACTACCTTCCTCTGCTTCTTCCTCTTCCTCTTCTTCCTCCTCCTCCTCTTCTTCTTCCACTTCCACTTCTTCCTCCACCTCCACTTCTTCATACTCTACCTCTTCCTCAGATGACTCTTCTTGATCGTTGTCCCCATCAAGGTCTATCTGCTCACCAGAATCTGTGGGTTTTTCCGGATCACTGTTTCCCCCTGTTGACTCTGAATTTTGGGCCGTTCCTTCTTTCTGCCTTGTCTCGGGCATTTTATGCATCTAGAAAAGCAAGCTTGCAGGGCAACTGATTAGAAGCAAAATCGAAATTGAAATGGAAGTAGAAGTTCTAGAATGAAGAGTTGATTTCGAAACCCTAGAATCGTAATCGCTGGCGCGTTCGTTGATCTTATGGTGGCCGAATTCACTATTGGTTGACGGTGTGCAGATGTTTGCAGTTGGGCCCTTTTTCCGGGGGTCCGGGGTCCCCTTTTTCCCCCCCCCCCCGGCGTTACCTTCCTGGCCGGTTTGAACTACCCACTTGCCTTT
TARGET=813,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1349
PRIMER_LEFT_PENALTY=0.132570
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=CTCATCTCGTCTTCACCATCTG
PRIMER_RIGHT_SEQUENCE=GAGACAAGGCAGAAAGAAGGAA
PRIMER_LEFT=749,22
PRIMER_RIGHT=1014,22
PRIMER_LEFT_TM=59.867
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.1372
PRIMER_LEFT_1_PENALTY=0.019797
PRIMER_RIGHT_1_PENALTY=0.117452
PRIMER_LEFT_1_SEQUENCE=ACCTTCCTCTGCTTCTTCCTCT
PRIMER_RIGHT_1_SEQUENCE=GGGACAACGATCAAGAAGAGTC
PRIMER_LEFT_1=781,22
PRIMER_RIGHT_1=911,22
PRIMER_LEFT_1_TM=60.020
PRIMER_RIGHT_1_TM=60.117
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.1839
PRIMER_LEFT_2_PENALTY=0.010545
PRIMER_RIGHT_2_PENALTY=0.173354
PRIMER_LEFT_2_SEQUENCE=TTCCACTCTCATCTCGTCTTCA
PRIMER_RIGHT_2_SEQUENCE=AGATAGACCTTGATGGGGACAA
PRIMER_LEFT_2=742,22
PRIMER_RIGHT_2=926,22
PRIMER_LEFT_2_TM=59.989
PRIMER_RIGHT_2_TM=59.827
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.1932
PRIMER_LEFT_3_PENALTY=0.019797
PRIMER_RIGHT_3_PENALTY=0.173354
PRIMER_LEFT_3_SEQUENCE=ACCTTCCTCTGCTTCTTCCTCT
PRIMER_RIGHT_3_SEQUENCE=AGATAGACCTTGATGGGGACAA
PRIMER_LEFT_3=781,22
PRIMER_RIGHT_3=926,22
PRIMER_LEFT_3_TM=60.020
PRIMER_RIGHT_3_TM=59.827
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.2763
PRIMER_LEFT_4_PENALTY=0.019797
PRIMER_RIGHT_4_PENALTY=0.256503
PRIMER_LEFT_4_SEQUENCE=ACCTTCCTCTGCTTCTTCCTCT
PRIMER_RIGHT_4_SEQUENCE=TTTGCTTCTAATCAGTTGCCCT
PRIMER_LEFT_4=781,22
PRIMER_RIGHT_4=1069,22
PRIMER_LEFT_4_TM=60.020
PRIMER_RIGHT_4_TM=60.257
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_01970 Ps_contig_mira-and-tgicl-ass_franssen_17777
SEQUENCE=GTGGTTTCTGAGAAAGTTGATACTCTCAGTCTATTCCCAATCAAGAACCTGGAGATAATTTGCAGAGTCCTACGTTGGGAGTGTGTCAATTATAATCTTCATAGAGAAACAATTGATCAGTCAAATTCTAGTCTATTGGAAAACAAAGAAGGAATATGCAAAGAAAACAATGAACATGTTTCTTTCTGAATACTGTTAGGGACTATGGACGTGTGCATTGGTACTGAAAATTAGTTGGTTAAGACATAAATATATATTAGGAGTGTGTGGTGTGTGTGTCAATACTAAATCATGTMTAGCGATATCATATGTCGGTTTGTTGGAGCCTGGTTATTTGTCTATACTATAGATAATTGGTTATATCTGTTTTGTATATA
TARGET=270,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01971 Ps_contig_mira-and-tgicl-ass_franssen_17797
SEQUENCE=CATTGTCGAAGGAGTGGAGAAGCATGAACCTCTTAATGAGGGTTCCATTCTCTGGCTGACTGAAAGCCGCAAACCATTAGGTTTAGTTGATGAAATCTTTGGACCAGTCAAAAACCCATATTATGTTGTGAGGTACAATTCTGAAAATGACATCCCTGCGGGAATCCAAGGGGGAACAACTTTGGTCTCATGTGTACCAGAATTTGCTGATCGTGTGCTTAATAATAATAAGGATCTTTAATAAGGAAAGGCTATGATGCGTTCG
TARGET=220,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2302
PRIMER_LEFT_PENALTY=0.115189
PRIMER_RIGHT_PENALTY=0.115007
PRIMER_LEFT_SEQUENCE=GAGTGGAGAAGCATGAACCTCT
PRIMER_RIGHT_SEQUENCE=AACGCATCATAGCCTTTCCTTA
PRIMER_LEFT=11,22
PRIMER_RIGHT=262,22
PRIMER_LEFT_TM=59.885
PRIMER_RIGHT_TM=60.115
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.3695
PRIMER_LEFT_1_PENALTY=0.254537
PRIMER_RIGHT_1_PENALTY=0.115007
PRIMER_LEFT_1_SEQUENCE=GAAGGAGTGGAGAAGCATGAAC
PRIMER_RIGHT_1_SEQUENCE=AACGCATCATAGCCTTTCCTTA
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=262,22
PRIMER_LEFT_1_TM=60.255
PRIMER_RIGHT_1_TM=60.115
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.4268
PRIMER_LEFT_2_PENALTY=0.311784
PRIMER_RIGHT_2_PENALTY=0.115007
PRIMER_LEFT_2_SEQUENCE=TAATGAGGGTTCCATTCTCTGG
PRIMER_RIGHT_2_SEQUENCE=AACGCATCATAGCCTTTCCTTA
PRIMER_LEFT_2=33,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=60.115
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.4425
PRIMER_LEFT_3_PENALTY=0.327468
PRIMER_RIGHT_3_PENALTY=0.115007
PRIMER_LEFT_3_SEQUENCE=ATTCTGAAAATGACATCCCTGC
PRIMER_RIGHT_3_SEQUENCE=AACGCATCATAGCCTTTCCTTA
PRIMER_LEFT_3=137,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=60.327
PRIMER_RIGHT_3_TM=60.115
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.4862
PRIMER_LEFT_4_PENALTY=0.115189
PRIMER_RIGHT_4_PENALTY=0.371060
PRIMER_LEFT_4_SEQUENCE=GAGTGGAGAAGCATGAACCTCT
PRIMER_RIGHT_4_SEQUENCE=CGCATCATAGCCTTTCCTTATT
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=260,22
PRIMER_LEFT_4_TM=59.885
PRIMER_RIGHT_4_TM=59.629
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=250
=
PRIMER_SEQUENCE_ID=SSR_01972 Ps_contig_mira-and-tgicl-ass_franssen_17798
SEQUENCE=TCTCTTTCAATGAAAACTTATTTTTATTGTTTCTTCTCCTGCCAGGATTTTGATCACTATTCCCTCTCTTTGTCATTCTCTGCAATTTCCTGTATTCAGCTTCTTTCTCATCATCCGAAAACTCTACTTCATCAGACAACTCTTCATCATTTGGACCAGATGCATCATAGCCTTTCCTATAAAGATCCTTATTATTATTAAGCACACGATCAGCAAATTCTGGTACACATGAGACCAAAGTTGTTCCCCCTTGGATTCCCGCAGGG
TARGET=189,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5168
PRIMER_LEFT_PENALTY=0.248900
PRIMER_RIGHT_PENALTY=0.267873
PRIMER_LEFT_SEQUENCE=TGTCATTCTCTGCAATTTCCTG
PRIMER_RIGHT_SEQUENCE=GGGAACAACTTTGGTCTCATGT
PRIMER_LEFT=70,22
PRIMER_RIGHT=247,22
PRIMER_LEFT_TM=60.249
PRIMER_RIGHT_TM=60.268
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.6501
PRIMER_LEFT_1_PENALTY=0.382228
PRIMER_RIGHT_1_PENALTY=0.267873
PRIMER_LEFT_1_SEQUENCE=CCCTCTCTTTGTCATTCTCTGC
PRIMER_RIGHT_1_SEQUENCE=GGGAACAACTTTGGTCTCATGT
PRIMER_LEFT_1=61,22
PRIMER_RIGHT_1=247,22
PRIMER_LEFT_1_TM=60.382
PRIMER_RIGHT_1_TM=60.268
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.7450
PRIMER_LEFT_2_PENALTY=0.382228
PRIMER_RIGHT_2_PENALTY=0.362762
PRIMER_LEFT_2_SEQUENCE=CCCTCTCTTTGTCATTCTCTGC
PRIMER_RIGHT_2_SEQUENCE=TACCAGAATTTGCTGATCGTGT
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=60.382
PRIMER_RIGHT_2_TM=59.637
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.7450
PRIMER_LEFT_3_PENALTY=0.382228
PRIMER_RIGHT_3_PENALTY=0.362762
PRIMER_LEFT_3_SEQUENCE=CCCTCTCTTTGTCATTCTCTGC
PRIMER_RIGHT_3_SEQUENCE=TGTACCAGAATTTGCTGATCGT
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=226,22
PRIMER_LEFT_3_TM=60.382
PRIMER_RIGHT_3_TM=59.637
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.7450
PRIMER_LEFT_4_PENALTY=0.382228
PRIMER_RIGHT_4_PENALTY=0.362762
PRIMER_LEFT_4_SEQUENCE=CCCTCTCTTTGTCATTCTCTGC
PRIMER_RIGHT_4_SEQUENCE=GTGTACCAGAATTTGCTGATCG
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=60.382
PRIMER_RIGHT_4_TM=59.637
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_01973 Ps_contig_mira-and-tgicl-ass_franssen_17810
SEQUENCE=CAACATGGAGCCATTGTTACCATTTTCACTTGTACAATCAAAATTCCAATTCATATCCTTCATCTACACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCTAGGAGAAGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCAKGKTTRTAAGYMTAMSTCACCTCCTCCGCCAGTTTACAAGTACAATTCACCTCCTCCACCTAGTTGTACAAGTATAATTCTCCTCCACCGCCAGTTTACAAATACAAATCACCTCCTCCCCCAGTGTACAAGTACAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAA
TARGET=117,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01974 Ps_contig_mira-and-tgicl-ass_franssen_17810
SEQUENCE=CAACATGGAGCCATTGTTACCATTTTCACTTGTACAATCAAAATTCCAATTCATATCCTTCATCTACACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCTAGGAGAAGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCAKGKTTRTAAGYMTAMSTCACCTCCTCCGCCAGTTTACAAGTACAATTCACCTCCTCCACCTAGTTGTACAAGTATAATTCTCCTCCACCGCCAGTTTACAAATACAAATCACCTCCTCCCCCAGTGTACAAGTACAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAA
TARGET=480,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01975 Ps_contig_mira-and-tgicl-ass_franssen_17816
SEQUENCE=CTTTCTGCTCTATGAGGCTTACACATCTCAAGATCCATTGAGTGTCTCAGTTGTTGTTTTGCCACAATTTAATATCCCTCCTGAAATGTATCAAGTTGGATTCACCAAACTGTATCTTCGAACAGAGAAGGACTATTGCATCCGTATTTATTGTCAATGAGATTAAACAGTGAGAAACAAAGGATGGTTGAGATACGGAAGGAACTTCGACCTCGGTCCTATAGAATTAAGAGAAATTGGAGCATTGTGTGCAATGAAGGAAACATAAATATTTTTTGATGATCCAAAATTTGCAGAGTATAAAGCAGTTAGAACAGGTAAGCTCCACTGCATGTTTGTAAATTTTATTTATTTATTACATGTA
TARGET=344,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01976 Ps_contig_mira-and-tgicl-ass_franssen_17818
SEQUENCE=AGAGTTGGATAACTAGATAGGTTCCAGTGTTTGGTAGTTAAGTTAAGTTACTTAGTTACTTAATTAACCTTTCTTGGAGCAACCAAATTCTGTTCTCCTTTTCTATCTCAGTCTTCTAGCAATTTATGTCAGCCAGCCAGAAGATCAAACACGCCCAATTGAATTACCTGAACTCTATAACAGGATTGGAATTCTTCATTCCCTTGGCTTCATAATATTGTGAGTTGGCCATTTAATCCTAATGACAGGGATGTGACAGATTTTGGTACCAA
TARGET=36,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.5056
PRIMER_LEFT_PENALTY=4.130509
PRIMER_RIGHT_PENALTY=0.375090
PRIMER_LEFT_SEQUENCE=AACTAGATAGGTTCCAGTGTTTGG
PRIMER_RIGHT_SEQUENCE=TGTCACATCCCTGTCATTAGGA
PRIMER_LEFT=10,24
PRIMER_RIGHT=257,22
PRIMER_LEFT_TM=57.869
PRIMER_RIGHT_TM=60.375
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=4.5621
PRIMER_LEFT_1_PENALTY=4.130509
PRIMER_RIGHT_1_PENALTY=0.431597
PRIMER_LEFT_1_SEQUENCE=AACTAGATAGGTTCCAGTGTTTGG
PRIMER_RIGHT_1_SEQUENCE=TTATGAAGCCAAGGGAATGAAG
PRIMER_LEFT_1=10,24
PRIMER_RIGHT_1=214,22
PRIMER_LEFT_1_TM=57.869
PRIMER_RIGHT_1_TM=60.432
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=4.6580
PRIMER_LEFT_2_PENALTY=4.130509
PRIMER_RIGHT_2_PENALTY=0.527462
PRIMER_LEFT_2_SEQUENCE=AACTAGATAGGTTCCAGTGTTTGG
PRIMER_RIGHT_2_SEQUENCE=CTGTCACATCCCTGTCATTAGG
PRIMER_LEFT_2=10,24
PRIMER_RIGHT_2=258,22
PRIMER_LEFT_2_TM=57.869
PRIMER_RIGHT_2_TM=59.473
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=4.6769
PRIMER_LEFT_3_PENALTY=4.130509
PRIMER_RIGHT_3_PENALTY=0.546410
PRIMER_LEFT_3_SEQUENCE=AACTAGATAGGTTCCAGTGTTTGG
PRIMER_RIGHT_3_SEQUENCE=AAAATCTGTCACATCCCTGTCA
PRIMER_LEFT_3=10,24
PRIMER_RIGHT_3=263,22
PRIMER_LEFT_3_TM=57.869
PRIMER_RIGHT_3_TM=59.454
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=4.7307
PRIMER_LEFT_4_PENALTY=4.355620
PRIMER_RIGHT_4_PENALTY=0.375090
PRIMER_LEFT_4_SEQUENCE=AACTAGATAGGTTCCAGTGTTTGGT
PRIMER_RIGHT_4_SEQUENCE=TGTCACATCCCTGTCATTAGGA
PRIMER_LEFT_4=10,25
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=58.644
PRIMER_RIGHT_4_TM=60.375
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_01977 Ps_contig_mira-and-tgicl-ass_franssen_17832
SEQUENCE=ACTTTTTTTAAACTTAATAACACCTTTTCAATGGCACGGCAGAGTAAAGATACATAGCAGCCATTAAGTGAGGTTTGGATAAAAAGTAAAAACAACTCAATCACATGATGATTGAAAATACCAATAAAATCTATNTNAAAAATAAAGTCAACGGTTCAACCACAAAACAACATAACATTACAGCATATAACGGCACAAATCAACACACATAGGCCAGCACTCTCGAAGGTCCTTCCTATTCAAAAGCATCGTCATCATCATCAGGAAGGGGCTGATTAGCAGCCTCAAGAATCTCATTCTCATGCCTTTGTTGTAAAGCAATATCAATTTGAACTTCTGGTGGAGCCAGTGCAGGAGATTCTACAAAGTGCAAATTAGGATCACCTGCAAGTTTCCTGGCAAGATACAGGAAAGGCTTCTCAAAGTTGTAGTTGCTCTTAGCTGATATTTCATAGTACTGCAAATTTTTCTTCCTGTGGAAGGTAACCTGCTTTGCCTTCACTTGCCTGTTCTTCACATCAACCTTGTTTCCACAAAGAACAATTGGGATGTTTTCACAGACCCGGCAAAGATCACGGTGCCAAGTAGGGACATTCTTGTATGTCAGCCGTGCAGTAACATCAAACATTATAATAGCACACTGTCCATGAATATAGTATCCATCTCTGAGACCACCAAACTTCTCTTGACCAGCAGTATCCCAGCAGTAGAAACGAATCTTACCACAGTTTGTGAAGAAATCCAATGGATGCACTTCAACACCAATAGTTGGTTCATATTTCTTCTCAAATTCTCCAGTAAGATGCCTCTTTACGAAAGTTGTTTTTCCTGTGCCACCGTCACCGACGATCACAAGCTTGAAACTAGGATAATCAACTGTTTGCTGATTAGGCAAGGCCATTGAAATGAATGGAATAATCGATTATGGAGGAAAAGCTAGGTTGCGTTGTGTTTCAGTGTCCCCGACGTACTCTGCGTTGTATTACCTTACTTTGTTTCTTTTT
TARGET=252,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2136
PRIMER_LEFT_PENALTY=0.203283
PRIMER_RIGHT_PENALTY=0.010294
PRIMER_LEFT_SEQUENCE=ATAACGGCACAAATCAACACAC
PRIMER_RIGHT_SEQUENCE=GAATGAGATTCTTGAGGCTGCT
PRIMER_LEFT=186,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=59.797
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=113
PRIMER_PAIR_PENALTY_1=0.2136
PRIMER_LEFT_1_PENALTY=0.203283
PRIMER_RIGHT_1_PENALTY=0.010294
PRIMER_LEFT_1_SEQUENCE=ATAACGGCACAAATCAACACAC
PRIMER_RIGHT_1_SEQUENCE=AGAATGAGATTCTTGAGGCTGC
PRIMER_LEFT_1=186,22
PRIMER_RIGHT_1=299,22
PRIMER_LEFT_1_TM=59.797
PRIMER_RIGHT_1_TM=59.990
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=0.3020
PRIMER_LEFT_2_PENALTY=0.291669
PRIMER_RIGHT_2_PENALTY=0.010294
PRIMER_LEFT_2_SEQUENCE=GGCACAAATCAACACACATAGG
PRIMER_RIGHT_2_SEQUENCE=GAATGAGATTCTTGAGGCTGCT
PRIMER_LEFT_2=191,22
PRIMER_RIGHT_2=298,22
PRIMER_LEFT_2_TM=60.292
PRIMER_RIGHT_2_TM=59.990
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.3020
PRIMER_LEFT_3_PENALTY=0.291669
PRIMER_RIGHT_3_PENALTY=0.010294
PRIMER_LEFT_3_SEQUENCE=ACAAATCAACACACATAGGCCA
PRIMER_RIGHT_3_SEQUENCE=GAATGAGATTCTTGAGGCTGCT
PRIMER_LEFT_3=194,22
PRIMER_RIGHT_3=298,22
PRIMER_LEFT_3_TM=60.292
PRIMER_RIGHT_3_TM=59.990
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.3020
PRIMER_LEFT_4_PENALTY=0.291669
PRIMER_RIGHT_4_PENALTY=0.010294
PRIMER_LEFT_4_SEQUENCE=GGCACAAATCAACACACATAGG
PRIMER_RIGHT_4_SEQUENCE=AGAATGAGATTCTTGAGGCTGC
PRIMER_LEFT_4=191,22
PRIMER_RIGHT_4=299,22
PRIMER_LEFT_4_TM=60.292
PRIMER_RIGHT_4_TM=59.990
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_01978 Ps_contig_mira-and-tgicl-ass_franssen_17873
SEQUENCE=GACAAACYRGRGACTGAAATCGAGAATCGAGAGCAATAATCGAACATCGTTTCAGAAAGTGAAACAGAAGAAGAAGAACCAGAGAGAGAATAATCAAGGAAGGTAAAAATGACGGAAGCGATGATCAGGAAGAAGCCAGGAATGGCCAGCGTGAAGGACATGCCGGTTTCTTCAGGACCGGTCCACCCGTCCCCGGGTCCGGTTTTCCGCCTTCCCCGGTTCCCGGGTTCCGCCTTCCGGTCCGAAACTTCCCCTAACACGTTCGGGTCCCGAGTCGCCCTATTTCG
TARGET=66,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01979 Ps_contig_mira-and-tgicl-ass_franssen_17877
SEQUENCE=TTAATACACATACAATAATCGTTTTAATTTTAGTATTATAATAAACAAAACCAAGAACTAACTACAATTTCTTTCACTACAACTACCATAATAACAACTTRAAATTATACAAYATCAATTACWAAATTAAAATTRGGGARRAAAAAACGARACCCTAAACAATGTTAACTTTAGCACCTGCACCTTCAAGTTGTTTCTTAGCATCTTCAGCTTCATCTTTCGAAACGCCTTCTTTGAATTTCTTAGGCAAACCTTCAATCAACTCCTTCGCTTCCTTCAATCCCAGATTCGTCAAAGCCCTAACCGCTTTAATCGCCGCAATTCTCGCGGTACTCGGAACTTCTTCAATAACCACATCGAATTCGGTTTGTTCTTCCACGACAGCAGCTTCCGCGGCGGCGGGAGCAGCAACGGCGGCGGCGGGAGCGAACGAAGCGGTCGGTTACGCCGTAGTTTGTCTTGGAAGTAGTCGACGTTTAGGGTTTTTGGCTTGTTCGAGTGTGGACGGG
TARGET=412,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01980 Ps_contig_mira-and-tgicl-ass_franssen_17880
SEQUENCE=TTAGCTGGTGTTATTGGTTGGTAGTAGGATCTGGTGGTTTCTATTGGATGACAATTTGGTAAAGGAAAAGGGAAAAAGAAAACAAAAATAAAGAATGACAGAGTAGAGTTTGTTGCTCATCTTATTCTTCTTCTGTGACAGATCCTTATAAGATATTAAAGGTTCAACCAGATGCTTCTKATWTCTTGATGTTAGAAAGGCTTTTAGACAACTTGCTTTGCAGTATCATCCAGATAGTTTAGCAGAGGAAAAGATTGTGATGTGCGGTTTCACGTAATCAATGAGGTCTTTATTGTTTGTTGCGATTACAAATTTGAGAGAAGAAACAAAGAAGAGAGAAACATATGAGAAGGAGAAGAAGAAGAAGAGGTGTTATGATGATGAAACCTATTTAAGGAAGGAAAGAATGATCCAGAGTTGGGGATAGTTAGGGAAGAAGTGGTAGG
TARGET=354,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01981 Ps_contig_mira-and-tgicl-ass_franssen_17882
SEQUENCE=AGATGTTTGCAGAGGAAAAGATTGTGATGTGCAGTTTCACGTAATCAATGAGGCTTATGTTGTTGCGATTACAAATTTGAGAGAAGAAACAAAGAAGAGAGAAACATATGAGAAGGAGAAGAAGAAGAAGAGGTGTTATGATGATGAACCTATTTAGAAGGAAAGAATGATCCAGAGTTGGGGGATTAGTTAGGGAAAGAATGGAGTTGGGTTAATAGGAAAGGGAGCGAAGGTAAATTAAGTAATGTGTACTTCTACTAATCTAATTAATTTAACTTCCCTTTCTATTTGATACTTAAACTTTA
TARGET=117,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1980
PRIMER_LEFT_PENALTY=0.138114
PRIMER_RIGHT_PENALTY=0.059875
PRIMER_LEFT_SEQUENCE=TCAATGAGGCTTATGTTGTTGC
PRIMER_RIGHT_SEQUENCE=CCAACTCTGGATCATTCTTTCC
PRIMER_LEFT=44,22
PRIMER_RIGHT=180,22
PRIMER_LEFT_TM=60.138
PRIMER_RIGHT_TM=59.940
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=0.1980
PRIMER_LEFT_1_PENALTY=0.138114
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=TCAATGAGGCTTATGTTGTTGC
PRIMER_RIGHT_1_SEQUENCE=CCCAACTCTGGATCATTCTTTC
PRIMER_LEFT_1=44,22
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=60.138
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=0.2074
PRIMER_LEFT_2_PENALTY=0.138114
PRIMER_RIGHT_2_PENALTY=0.069319
PRIMER_LEFT_2_SEQUENCE=TCAATGAGGCTTATGTTGTTGC
PRIMER_RIGHT_2_SEQUENCE=TTCCCTAACTAATCCCCCAACT
PRIMER_LEFT_2=44,22
PRIMER_RIGHT_2=196,22
PRIMER_LEFT_2_TM=60.138
PRIMER_RIGHT_2_TM=60.069
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=153
PRIMER_PAIR_PENALTY_3=0.2134
PRIMER_LEFT_3_PENALTY=0.138114
PRIMER_RIGHT_3_PENALTY=0.075241
PRIMER_LEFT_3_SEQUENCE=TCAATGAGGCTTATGTTGTTGC
PRIMER_RIGHT_3_SEQUENCE=AACTAATCCCCCAACTCTGGAT
PRIMER_LEFT_3=44,22
PRIMER_RIGHT_3=190,22
PRIMER_LEFT_3_TM=60.138
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=0.2769
PRIMER_LEFT_4_PENALTY=0.217054
PRIMER_RIGHT_4_PENALTY=0.059875
PRIMER_LEFT_4_SEQUENCE=TTGTGATGTGCAGTTTCACGTA
PRIMER_RIGHT_4_SEQUENCE=CCAACTCTGGATCATTCTTTCC
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=180,22
PRIMER_LEFT_4_TM=60.217
PRIMER_RIGHT_4_TM=59.940
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_01982 Ps_contig_mira-and-tgicl-ass_franssen_17883
SEQUENCE=TTAAACTAAAAAACTAACCTACTCGGGTTCGGTTTTGTTAGTTTTGGTTGGTTTGGTATTATTTTCTAAAGAATGTACCCGTTTTTAACGGTAAAGACTTTCTGGGTTTTTGAGGGTTTTTGGTGAGTTTTTAAGTAAATGGCTGCTGCTACTATTGTTGTTGGTGGTAACGGAGCNGGTGTTTCATGGATGCAATTTGGAAGAAAGGAAACGAAACAAAATAAGATGAATAGAGTTAGAGTTTGTTGCTCATCTTATTCTTCTTCTGTGACAGATCCTTATAAGATATTAAAGATTCAACCAGACGCTTCTGAATCTGATGTTAGAAAGGCTTTTAGACAGCTTGCTTTGCAGGTTTCATTCTATATCCTATGTTTTTGTTCCCAAAAGTTAGTTTCTTTAAGATTTATGTATTTTCATTTTGATTGGGTTTTATTTGTGTTTCAGTATCATCCAGATGTTTGCAGAGGAAAAGATTGTGATGTGCGGTTTCACGTAATCAATGAGGCTTATGTTGTGAGTTATTATTATCTATAAATTTTSATTGCAAATAGTGAATTGAATTAGTAGTAATAATATGAATGAAATGATGTTCAGGTTGCGATTACAAATTTGAGAGAAGAAACAAAGAAGAGAGAAACATATGAGAAGGAGAAGAAGAAGAAGAGGTGTTATGATGATGAACCTATTTAAGGAAGGRAWRAKCCAGATTGGGGATATTGGGAAGAATGGNTGGGTTATGAAGGAGCAGGAATTAGTAATAGTGCTTCTCTAATCATATTAATTCCTTTTCTATTTGATCAACTTTATTGTTATAGTAAATTAAGTATTTAAACTTTAAACCCTTTTTAAACTTTAATTA
TARGET=653,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01983 Ps_contig_mira-and-tgicl-ass_franssen_17886
SEQUENCE=CCCTTTCCTATAAACCCCTAACTCCCACTTCTTTCCCCTAACTATCCCCAACTCTGGATCATTCTTTCCTTCTAAATGGTTCATCATCATAACACCTCTTCTTCTTCTTCTCCTTCTCATATGTTTCTCTCTTCTTTGTTTCTTCTCTCAAATTTGTAATCGCAACAACATAAGCCTCATTGATTACGTGAAACCGCACATCACAATCTTTTCCTCTGCAAACATCTGGATGATACTTGCAAAGCAAGTTGTCTAAAAGCCTTTCTAACAAATAACAAAGAAATTAACAAGAAAGCATCTGGTNAACCTTTANTCTTATAAGGATCTGTCACAGAAGAAGAATAAGATGAGCAAACAACTCTAACTNTGTTCATCTTTATTTTGTTTTCCTTTCCTTTCTACAATTGC
TARGET=97,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1425
PRIMER_LEFT_PENALTY=0.004430
PRIMER_RIGHT_PENALTY=0.138114
PRIMER_LEFT_SEQUENCE=CTAACTCCCACTTCTTTCCCCT
PRIMER_RIGHT_SEQUENCE=TCAATGAGGCTTATGTTGTTGC
PRIMER_LEFT=17,22
PRIMER_RIGHT=182,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.138
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.1425
PRIMER_LEFT_1_PENALTY=0.004430
PRIMER_RIGHT_1_PENALTY=0.138114
PRIMER_LEFT_1_SEQUENCE=CTCCCACTTCTTTCCCCTAACT
PRIMER_RIGHT_1_SEQUENCE=TCAATGAGGCTTATGTTGTTGC
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=182,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=60.138
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.1980
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.138114
PRIMER_LEFT_2_SEQUENCE=CCAACTCTGGATCATTCTTTCC
PRIMER_RIGHT_2_SEQUENCE=TCAATGAGGCTTATGTTGTTGC
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=182,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=60.138
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.1980
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.138114
PRIMER_LEFT_3_SEQUENCE=CCCAACTCTGGATCATTCTTTC
PRIMER_RIGHT_3_SEQUENCE=TCAATGAGGCTTATGTTGTTGC
PRIMER_LEFT_3=46,22
PRIMER_RIGHT_3=182,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=60.138
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=137
PRIMER_PAIR_PENALTY_4=0.2074
PRIMER_LEFT_4_PENALTY=0.069319
PRIMER_RIGHT_4_PENALTY=0.138114
PRIMER_LEFT_4_SEQUENCE=TTTCCCCTAACTATCCCCAACT
PRIMER_RIGHT_4_SEQUENCE=TCAATGAGGCTTATGTTGTTGC
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=182,22
PRIMER_LEFT_4_TM=60.069
PRIMER_RIGHT_4_TM=60.138
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_01984 Ps_contig_mira-and-tgicl-ass_franssen_17889
SEQUENCE=CTCCTCACAAACACAACCCTTAACAATGGCACTTAGGATGTGGGCTTCTTCAACTGCTAATGCACTCAAACTCTCCTCTTCCTCTAGATTACATCTCTCCCCTACCTTTTCCATCTCCAGATGTTTCTCTAATGGTATTTCTCTTCACATTTTACCTTTTATATTGAATCATCAAACATATTTATTTCTTGCTGAATTTGCAGTATTGGATGGACTCAAGTACGCACCTTCACATGAATGGGTCAAGCATGAAGGATCAGTAGCCACTATTGGTATCACTGACCATGCCCAGGTTTGTTTCCTCCACCTCTATATGTATGTTGTTCTGTCTGTGCTTTATTGTTTGTTTGTTACAGGACCATCTTGGAGAGGTTGTGTTTGTGGAGCTTCCAGAACCTGGTGTCTCAGTTACAAAGGGTAAAGGTTTCGGAGCTGTTGGAAAGTGTGAAGGCAACAAGTGATGTAAACTCTCCTATTTCCGGTGAGGTTATCGAGGTTAATACTGGGCTCACCGGAAAGCCTGGCCTCGTAAGTTCATTCCTTCACTCTCACCTTTAGGACTAATTATAACACTTATCTCAACTGGTTTGAGTTAAGATCTGAGTTCAAACTCTGATGATGAACATAACAAGTAATTAGTACTAGTATTTACCGTTTGAGAAAGTAACTTAATTTTTCTACTTTTGTTGCTTGAAGATCAATTCAAGCCCCTATGAAGATGGATGGATGATAAAAATAAAGCCAACTAGTCCAGATGAATTAGAATCCTTGTTGGGTGCAAAAGAGTACACAAAGTTTTGTGAGGAAGAAGATGCTGCACATTGATGTCAATTTTTCAATATATTGAACTGACTGTTTTTAATATTGTACTACTATCTATCATCCTGTTGTCTATTTTTTTTCAAATGCAATAGAATTGCTTCAAGTTATTGTTGTTTCCATGCGACACTTCAGTGTGTCAGTCTATCTAGCTTGTCAT
TARGET=340,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1727
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=0.040447
PRIMER_LEFT_SEQUENCE=TGCAGTATTGGATGGACTCAAG
PRIMER_RIGHT_SEQUENCE=TAACCTCACCGGAAATAGGAGA
PRIMER_LEFT=198,22
PRIMER_RIGHT=489,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=59.960
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=0.2131
PRIMER_LEFT_1_PENALTY=0.132262
PRIMER_RIGHT_1_PENALTY=0.080808
PRIMER_LEFT_1_SEQUENCE=TGCAGTATTGGATGGACTCAAG
PRIMER_RIGHT_1_SEQUENCE=ACATCACTTGTTGCCTTCACAC
PRIMER_LEFT_1=198,22
PRIMER_RIGHT_1=463,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.2240
PRIMER_LEFT_2_PENALTY=0.038744
PRIMER_RIGHT_2_PENALTY=0.185267
PRIMER_LEFT_2_SEQUENCE=CTCTCCCCTACCTTTTCCATCT
PRIMER_RIGHT_2_SEQUENCE=CACAAACACAACCTCTCCAAGA
PRIMER_LEFT_2=94,22
PRIMER_RIGHT_2=382,22
PRIMER_LEFT_2_TM=59.961
PRIMER_RIGHT_2_TM=60.185
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.2481
PRIMER_LEFT_3_PENALTY=0.038744
PRIMER_RIGHT_3_PENALTY=0.209343
PRIMER_LEFT_3_SEQUENCE=CTCTCCCCTACCTTTTCCATCT
PRIMER_RIGHT_3_SEQUENCE=AAGCTCCACAAACACAACCTCT
PRIMER_LEFT_3=94,22
PRIMER_RIGHT_3=388,22
PRIMER_LEFT_3_TM=59.961
PRIMER_RIGHT_3_TM=60.209
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=0.2597
PRIMER_LEFT_4_PENALTY=0.132262
PRIMER_RIGHT_4_PENALTY=0.127450
PRIMER_LEFT_4_SEQUENCE=TGCAGTATTGGATGGACTCAAG
PRIMER_RIGHT_4_SEQUENCE=TCCGAAACCTTTACCCTTTGTA
PRIMER_LEFT_4=198,22
PRIMER_RIGHT_4=430,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=59.873
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_01985 Ps_contig_mira-and-tgicl-ass_franssen_17889
SEQUENCE=CTCCTCACAAACACAACCCTTAACAATGGCACTTAGGATGTGGGCTTCTTCAACTGCTAATGCACTCAAACTCTCCTCTTCCTCTAGATTACATCTCTCCCCTACCTTTTCCATCTCCAGATGTTTCTCTAATGGTATTTCTCTTCACATTTTACCTTTTATATTGAATCATCAAACATATTTATTTCTTGCTGAATTTGCAGTATTGGATGGACTCAAGTACGCACCTTCACATGAATGGGTCAAGCATGAAGGATCAGTAGCCACTATTGGTATCACTGACCATGCCCAGGTTTGTTTCCTCCACCTCTATATGTATGTTGTTCTGTCTGTGCTTTATTGTTTGTTTGTTACAGGACCATCTTGGAGAGGTTGTGTTTGTGGAGCTTCCAGAACCTGGTGTCTCAGTTACAAAGGGTAAAGGTTTCGGAGCTGTTGGAAAGTGTGAAGGCAACAAGTGATGTAAACTCTCCTATTTCCGGTGAGGTTATCGAGGTTAATACTGGGCTCACCGGAAAGCCTGGCCTCGTAAGTTCATTCCTTCACTCTCACCTTTAGGACTAATTATAACACTTATCTCAACTGGTTTGAGTTAAGATCTGAGTTCAAACTCTGATGATGAACATAACAAGTAATTAGTACTAGTATTTACCGTTTGAGAAAGTAACTTAATTTTTCTACTTTTGTTGCTTGAAGATCAATTCAAGCCCCTATGAAGATGGATGGATGATAAAAATAAAGCCAACTAGTCCAGATGAATTAGAATCCTTGTTGGGTGCAAAAGAGTACACAAAGTTTTGTGAGGAAGAAGATGCTGCACATTGATGTCAATTTTTCAATATATTGAACTGACTGTTTTTAATATTGTACTACTATCTATCATCCTGTTGTCTATTTTTTTTCAAATGCAATAGAATTGCTTCAAGTTATTGTTGTTTCCATGCGACACTTCAGTGTGTCAGTCTATCTAGCTTGTCAT
TARGET=718,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3000
PRIMER_LEFT_PENALTY=0.069526
PRIMER_RIGHT_PENALTY=0.230523
PRIMER_LEFT_SEQUENCE=AGATCAATTCAAGCCCCTATGA
PRIMER_RIGHT_SEQUENCE=ACATCAATGTGCAGCATCTTCT
PRIMER_LEFT=694,22
PRIMER_RIGHT=827,22
PRIMER_LEFT_TM=59.930
PRIMER_RIGHT_TM=59.769
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.3479
PRIMER_LEFT_1_PENALTY=0.069526
PRIMER_RIGHT_1_PENALTY=0.278329
PRIMER_LEFT_1_SEQUENCE=AGATCAATTCAAGCCCCTATGA
PRIMER_RIGHT_1_SEQUENCE=CATCAATGTGCAGCATCTTCTT
PRIMER_LEFT_1=694,22
PRIMER_RIGHT_1=826,22
PRIMER_LEFT_1_TM=59.930
PRIMER_RIGHT_1_TM=60.278
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=0.5757
PRIMER_LEFT_2_PENALTY=0.069526
PRIMER_RIGHT_2_PENALTY=0.506150
PRIMER_LEFT_2_SEQUENCE=AGATCAATTCAAGCCCCTATGA
PRIMER_RIGHT_2_SEQUENCE=CAGCATCTTCTTCCTCACAAAA
PRIMER_LEFT_2=694,22
PRIMER_RIGHT_2=816,22
PRIMER_LEFT_2_TM=59.930
PRIMER_RIGHT_2_TM=59.494
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.6356
PRIMER_LEFT_3_PENALTY=0.069526
PRIMER_RIGHT_3_PENALTY=0.566078
PRIMER_LEFT_3_SEQUENCE=AGATCAATTCAAGCCCCTATGA
PRIMER_RIGHT_3_SEQUENCE=TTTGTGTACTCTTTTGCACCCA
PRIMER_LEFT_3=694,22
PRIMER_RIGHT_3=793,22
PRIMER_LEFT_3_TM=59.930
PRIMER_RIGHT_3_TM=60.566
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=0.6814
PRIMER_LEFT_4_PENALTY=0.069526
PRIMER_RIGHT_4_PENALTY=0.611852
PRIMER_LEFT_4_SEQUENCE=AGATCAATTCAAGCCCCTATGA
PRIMER_RIGHT_4_SEQUENCE=GTCGCATGGAAACAACAATAAC
PRIMER_LEFT_4=694,22
PRIMER_RIGHT_4=946,22
PRIMER_LEFT_4_TM=59.930
PRIMER_RIGHT_4_TM=59.388
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_01986 Ps_contig_mira-and-tgicl-ass_franssen_17890
SEQUENCE=TTTATTTKGATKCGAAAAAACAAAATTTAGTCTTCTTTAATTAGTACATGACAAGCTAGATAGACTGACACACTGAAGTGTCGCATGGAAGCAACAATAACTTGAAGCAATTCTATTGCATTTGAAAAAAAATAGACAACAGGATGATAGATAGTAGTACAATATTAAAAACAGTCAGTTCAATATAGTGAAAAATTGACATCAATGTGCAGCATCTTCTTCCTCACAAAACTTTGTGTACTCTTTTGCACCCAACAAGGATTCTAATTCATCTGGACTAGTTGGCTTTATTTTTATCATCCATCCATCTTCATAGGGGCTTGAATTGATCTTCAAGCAACAAAAGTAGAAAAATTAAGTTACTTTCTCAAACGGTAAATCTAGTAGTAATTACTTGTTATGTTAGTTTTTCGCTGTCATAACTCAGATCCAGTTGAGTTATCCAACCTACATAAGTTGTTTTCGGCTTATTTTTCATATTGAAATAAGTAGTTAAGTAATTAAGCTTTTAGTCGTAAAGGTGAGAGGAAGGAATGAACTTACGAGGCCAGGCTTTCCGGTGAGCCCCGTATTAACTTCGATAACCTCACCGGAAATAGGAGAGTTTACATCACTTGTTGCCTTCACACTTTCAACAGCTCCGAAACCTTTACCCTTTGTAACTGAGACACCAGGTTCTGGAAGCTCCACAAACACAACCTCTCCAAGATGGTCCTGCAAACAAACAAAAAGCACAGACAGACAAATACATATAGGGTGGAGGAAACAAACCTGGGCATGGTCAGTGATACCAATAGTGGCTACTGTCCTTATGCTTGACCCATTCATGTGAAGGTGCGTACTTGAGTCCATCCAATACTGCAAATTCAGCAAGAATAAATATGTTTGATGATTCAATATAAAAGTAAAATGTGAAGAGAAATACCATAGAGAAACATCTGGAATGAAAAGGTAGGGGAGAGATGTAATCTAGAGGAAGAGGAGAGTTTGAGTGCATTAGCAGTTGAAGAAGCCCACATCCTAAGTGCCATTGTTATTGTTAAGGGTTGTGTTTGTGAGGAGTGTGTAGAAGAATGAAATGAAATGATAGAAGACCCCGA
TARGET=298,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01987 Ps_contig_mira-and-tgicl-ass_franssen_17890
SEQUENCE=TTTATTTKGATKCGAAAAAACAAAATTTAGTCTTCTTTAATTAGTACATGACAAGCTAGATAGACTGACACACTGAAGTGTCGCATGGAAGCAACAATAACTTGAAGCAATTCTATTGCATTTGAAAAAAAATAGACAACAGGATGATAGATAGTAGTACAATATTAAAAACAGTCAGTTCAATATAGTGAAAAATTGACATCAATGTGCAGCATCTTCTTCCTCACAAAACTTTGTGTACTCTTTTGCACCCAACAAGGATTCTAATTCATCTGGACTAGTTGGCTTTATTTTTATCATCCATCCATCTTCATAGGGGCTTGAATTGATCTTCAAGCAACAAAAGTAGAAAAATTAAGTTACTTTCTCAAACGGTAAATCTAGTAGTAATTACTTGTTATGTTAGTTTTTCGCTGTCATAACTCAGATCCAGTTGAGTTATCCAACCTACATAAGTTGTTTTCGGCTTATTTTTCATATTGAAATAAGTAGTTAAGTAATTAAGCTTTTAGTCGTAAAGGTGAGAGGAAGGAATGAACTTACGAGGCCAGGCTTTCCGGTGAGCCCCGTATTAACTTCGATAACCTCACCGGAAATAGGAGAGTTTACATCACTTGTTGCCTTCACACTTTCAACAGCTCCGAAACCTTTACCCTTTGTAACTGAGACACCAGGTTCTGGAAGCTCCACAAACACAACCTCTCCAAGATGGTCCTGCAAACAAACAAAAAGCACAGACAGACAAATACATATAGGGTGGAGGAAACAAACCTGGGCATGGTCAGTGATACCAATAGTGGCTACTGTCCTTATGCTTGACCCATTCATGTGAAGGTGCGTACTTGAGTCCATCCAATACTGCAAATTCAGCAAGAATAAATATGTTTGATGATTCAATATAAAAGTAAAATGTGAAGAGAAATACCATAGAGAAACATCTGGAATGAAAAGGTAGGGGAGAGATGTAATCTAGAGGAAGAGGAGAGTTTGAGTGCATTAGCAGTTGAAGAAGCCCACATCCTAAGTGCCATTGTTATTGTTAAGGGTTGTGTTTGTGAGGAGTGTGTAGAAGAATGAAATGAAATGATAGAAGACCCCGA
TARGET=718,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01988 Ps_contig_mira-and-tgicl-ass_franssen_17890
SEQUENCE=TTTATTTKGATKCGAAAAAACAAAATTTAGTCTTCTTTAATTAGTACATGACAAGCTAGATAGACTGACACACTGAAGTGTCGCATGGAAGCAACAATAACTTGAAGCAATTCTATTGCATTTGAAAAAAAATAGACAACAGGATGATAGATAGTAGTACAATATTAAAAACAGTCAGTTCAATATAGTGAAAAATTGACATCAATGTGCAGCATCTTCTTCCTCACAAAACTTTGTGTACTCTTTTGCACCCAACAAGGATTCTAATTCATCTGGACTAGTTGGCTTTATTTTTATCATCCATCCATCTTCATAGGGGCTTGAATTGATCTTCAAGCAACAAAAGTAGAAAAATTAAGTTACTTTCTCAAACGGTAAATCTAGTAGTAATTACTTGTTATGTTAGTTTTTCGCTGTCATAACTCAGATCCAGTTGAGTTATCCAACCTACATAAGTTGTTTTCGGCTTATTTTTCATATTGAAATAAGTAGTTAAGTAATTAAGCTTTTAGTCGTAAAGGTGAGAGGAAGGAATGAACTTACGAGGCCAGGCTTTCCGGTGAGCCCCGTATTAACTTCGATAACCTCACCGGAAATAGGAGAGTTTACATCACTTGTTGCCTTCACACTTTCAACAGCTCCGAAACCTTTACCCTTTGTAACTGAGACACCAGGTTCTGGAAGCTCCACAAACACAACCTCTCCAAGATGGTCCTGCAAACAAACAAAAAGCACAGACAGACAAATACATATAGGGTGGAGGAAACAAACCTGGGCATGGTCAGTGATACCAATAGTGGCTACTGTCCTTATGCTTGACCCATTCATGTGAAGGTGCGTACTTGAGTCCATCCAATACTGCAAATTCAGCAAGAATAAATATGTTTGATGATTCAATATAAAAGTAAAATGTGAAGAGAAATACCATAGAGAAACATCTGGAATGAAAAGGTAGGGGAGAGATGTAATCTAGAGGAAGAGGAGAGTTTGAGTGCATTAGCAGTTGAAGAAGCCCACATCCTAAGTGCCATTGTTATTGTTAAGGGTTGTGTTTGTGAGGAGTGTGTAGAAGAATGAAATGAAATGATAGAAGACCCCGA
TARGET=1073,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01989 Ps_contig_mira-and-tgicl-ass_franssen_17894
SEQUENCE=AGCTGTTGAAAGTGTGAAGGCAACAAGTTGTATTGTTAAACTTCTTCCTTATTTCCGGTGAGGTTATCGAGGTTAATACTGGGCTCACCGGAAAGCCTGGCCTCATCAATTCAAGCCCCTATGAAGATGGATGGATGATAAAAATAAAGCCAACTAGTCCAGATGAATTAGAATCCTTGTTGGGTGCAAAAAGAGTACACAAAGTTTTGTGAAGGAAGAAGATGCTGCACATTGATGTTCTATTTTTCAATATTATTAGAACTGTTACTTGTTTTTAATATTGTACCTTATATCTTATCATTCTTGTTGTCTTTATTTTTTTAACTAAATAGACGAAATTAAGGTAAA
TARGET=126,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2892
PRIMER_LEFT_PENALTY=0.141882
PRIMER_RIGHT_PENALTY=0.147302
PRIMER_LEFT_SEQUENCE=CTTATTTCCGGTGAGGTTATCG
PRIMER_RIGHT_SEQUENCE=AACATCAATGTGCAGCATCTTC
PRIMER_LEFT=47,22
PRIMER_RIGHT=238,22
PRIMER_LEFT_TM=59.858
PRIMER_RIGHT_TM=60.147
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=0.3095
PRIMER_LEFT_1_PENALTY=0.162220
PRIMER_RIGHT_1_PENALTY=0.147302
PRIMER_LEFT_1_SEQUENCE=TTCCTTATTTCCGGTGAGGTTA
PRIMER_RIGHT_1_SEQUENCE=AACATCAATGTGCAGCATCTTC
PRIMER_LEFT_1=44,22
PRIMER_RIGHT_1=238,22
PRIMER_LEFT_1_TM=59.838
PRIMER_RIGHT_1_TM=60.147
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=0.3281
PRIMER_LEFT_2_PENALTY=0.180765
PRIMER_RIGHT_2_PENALTY=0.147302
PRIMER_LEFT_2_SEQUENCE=CTGTTGAAAGTGTGAAGGCAAC
PRIMER_RIGHT_2_SEQUENCE=AACATCAATGTGCAGCATCTTC
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=238,22
PRIMER_LEFT_2_TM=59.819
PRIMER_RIGHT_2_TM=60.147
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=0.3281
PRIMER_LEFT_3_PENALTY=0.180765
PRIMER_RIGHT_3_PENALTY=0.147302
PRIMER_LEFT_3_SEQUENCE=TGAAAGTGTGAAGGCAACAAGT
PRIMER_RIGHT_3_SEQUENCE=AACATCAATGTGCAGCATCTTC
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=59.819
PRIMER_RIGHT_3_TM=60.147
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.3369
PRIMER_LEFT_4_PENALTY=0.189596
PRIMER_RIGHT_4_PENALTY=0.147302
PRIMER_LEFT_4_SEQUENCE=GTTGAAAGTGTGAAGGCAACAA
PRIMER_RIGHT_4_SEQUENCE=AACATCAATGTGCAGCATCTTC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=238,22
PRIMER_LEFT_4_TM=60.190
PRIMER_RIGHT_4_TM=60.147
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_01990 Ps_contig_mira-and-tgicl-ass_franssen_17896
SEQUENCE=AAGTGATGTAAACTCTCCTATTTCCGGTGTTATCGAGGTTAATACTGGGCTCACCGGAAAGCCTGGCCTCATCAATTCAAGCCCCTATGAAGATGGATGGATGATAAAAATAAAGCCAACTAGTCCAGATGAATTAGAATCCTTGTTGGGTGACAAAAAGAAGTACAACAAAGTTTTGTGAGGAAGAAGATGCTGCACAGTGATGTTCTATTTTTCAATATATTGAACTGACTTGTTTTAATTATTGTACTACTATCTATCATCTTTGTTTGTTCTTTATTTTTTTACTTTAAATAGCAATAAGTAATTTACG
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3189
PRIMER_LEFT_PENALTY=0.263023
PRIMER_RIGHT_PENALTY=0.055853
PRIMER_LEFT_SEQUENCE=TATTTCCGGTGTTATCGAGGTT
PRIMER_RIGHT_SEQUENCE=ACATCACTGTGCAGCATCTTCT
PRIMER_LEFT=18,22
PRIMER_RIGHT=205,22
PRIMER_LEFT_TM=59.737
PRIMER_RIGHT_TM=59.944
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.3189
PRIMER_LEFT_1_PENALTY=0.263023
PRIMER_RIGHT_1_PENALTY=0.055853
PRIMER_LEFT_1_SEQUENCE=TTCCGGTGTTATCGAGGTTAAT
PRIMER_RIGHT_1_SEQUENCE=ACATCACTGTGCAGCATCTTCT
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=59.737
PRIMER_RIGHT_1_TM=59.944
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.3189
PRIMER_LEFT_2_PENALTY=0.263023
PRIMER_RIGHT_2_PENALTY=0.055853
PRIMER_LEFT_2_SEQUENCE=TATTTCCGGTGTTATCGAGGTT
PRIMER_RIGHT_2_SEQUENCE=AGAACATCACTGTGCAGCATCT
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=208,22
PRIMER_LEFT_2_TM=59.737
PRIMER_RIGHT_2_TM=59.944
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.3189
PRIMER_LEFT_3_PENALTY=0.263023
PRIMER_RIGHT_3_PENALTY=0.055853
PRIMER_LEFT_3_SEQUENCE=ATTTCCGGTGTTATCGAGGTTA
PRIMER_RIGHT_3_SEQUENCE=ACATCACTGTGCAGCATCTTCT
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=59.737
PRIMER_RIGHT_3_TM=59.944
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.3189
PRIMER_LEFT_4_PENALTY=0.263023
PRIMER_RIGHT_4_PENALTY=0.055853
PRIMER_LEFT_4_SEQUENCE=TTCCGGTGTTATCGAGGTTAAT
PRIMER_RIGHT_4_SEQUENCE=AGAACATCACTGTGCAGCATCT
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=208,22
PRIMER_LEFT_4_TM=59.737
PRIMER_RIGHT_4_TM=59.944
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_01991 Ps_contig_mira-and-tgicl-ass_franssen_17901
SEQUENCE=CTTCCTACGGCTGAAAAGGTTCGGAGGTTNTGCAACCCTGCATACCCTGGCGGACTTTCTTTAACCCTCTCGGTTTCGGTAAAGATGAGAAATCTTTGAAGGAGTTGAAGCTTAAGGAAGTGAAAAATGGAAGGTTAGCTATGTTGGCAATATTGGGATACTTCATTCAAGGTCTTGTTACAGGAGTTGGACCATACCAAAAACTTGTTAGATCATGTGGCTGATCCTGTCAACAACAATGTCTTGACAAGTCTCAAGTTTCATTGAATGAATGTATTATATTATATAATATATATATATATGTTGTTAATTACCTATATTCTTTTCTTTGCTCTTGGTTGTTGTAACACATTATTATTATACAAAAAACTACAACCATTGTTTTAGTTCATTCTCTTGTCTTTTGGGAGTGTGAACATGTTGTTTCATGTTGGAGCCTTGTAAGAAATGACATGTAAAAGAAACTAGAAATAATTTGGC
TARGET=289,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2867
PRIMER_LEFT_PENALTY=0.037936
PRIMER_RIGHT_PENALTY=0.248781
PRIMER_LEFT_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_RIGHT_SEQUENCE=TTCACACTCCCAAAAGACAAGA
PRIMER_LEFT=205,22
PRIMER_RIGHT=415,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=59.751
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=0.2914
PRIMER_LEFT_1_PENALTY=0.037936
PRIMER_RIGHT_1_PENALTY=0.253482
PRIMER_LEFT_1_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_RIGHT_1_SEQUENCE=TGTCATTTCTTACAAGGCTCCA
PRIMER_LEFT_1=205,22
PRIMER_RIGHT_1=452,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=59.747
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.2914
PRIMER_LEFT_2_PENALTY=0.037936
PRIMER_RIGHT_2_PENALTY=0.253482
PRIMER_LEFT_2_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_RIGHT_2_SEQUENCE=TCTTACAAGGCTCCAACATGAA
PRIMER_LEFT_2=205,22
PRIMER_RIGHT_2=445,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=59.747
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=0.2914
PRIMER_LEFT_3_PENALTY=0.037936
PRIMER_RIGHT_3_PENALTY=0.253482
PRIMER_LEFT_3_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_RIGHT_3_SEQUENCE=TTCTTACAAGGCTCCAACATGA
PRIMER_LEFT_3=205,22
PRIMER_RIGHT_3=446,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=59.747
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.3446
PRIMER_LEFT_4_PENALTY=0.095771
PRIMER_RIGHT_4_PENALTY=0.248781
PRIMER_LEFT_4_SEQUENCE=TTAGATCATGTGGCTGATCCTG
PRIMER_RIGHT_4_SEQUENCE=TTCACACTCCCAAAAGACAAGA
PRIMER_LEFT_4=207,22
PRIMER_RIGHT_4=415,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_01992 Ps_contig_mira-and-tgicl-ass_franssen_17903
SEQUENCE=AAAGACAAGTAGGTAATACAACGCAGAGTCGTCGGGGTTGGCAATATTGGATACTTCATTCAAGGTCTTGTTACAGGAGTTGGACCAATAACCTAAAACTTGTTAGATCATGTGGCTGATCCTGTCAACAACAATGTCTTGACAAGTCTCAAGTTTCATTGAATGAATGTATTATATTATATAATATATATATATGTTGTTAATTACCTATATTCTTTTCTTTGCTCTTGGTTGTTGTAACACATTATTATCAAAAAAACTCAACCATGTTTTAGTTCATTCTCTTGTCTTTTGGGAGTGTGAACATGTTGTTTTCATTGGTTGGAGT
TARGET=184,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2867
PRIMER_LEFT_PENALTY=0.037936
PRIMER_RIGHT_PENALTY=0.248781
PRIMER_LEFT_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_RIGHT_SEQUENCE=TTCACACTCCCAAAAGACAAGA
PRIMER_LEFT=100,22
PRIMER_RIGHT=303,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=59.751
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=0.3446
PRIMER_LEFT_1_PENALTY=0.095771
PRIMER_RIGHT_1_PENALTY=0.248781
PRIMER_LEFT_1_SEQUENCE=TTAGATCATGTGGCTGATCCTG
PRIMER_RIGHT_1_SEQUENCE=TTCACACTCCCAAAAGACAAGA
PRIMER_LEFT_1=102,22
PRIMER_RIGHT_1=303,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.751
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.3504
PRIMER_LEFT_2_PENALTY=0.037936
PRIMER_RIGHT_2_PENALTY=0.312496
PRIMER_LEFT_2_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_RIGHT_2_SEQUENCE=ACAACATGTTCACACTCCCAAA
PRIMER_LEFT_2=100,22
PRIMER_RIGHT_2=311,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=60.312
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.3504
PRIMER_LEFT_3_PENALTY=0.037936
PRIMER_RIGHT_3_PENALTY=0.312496
PRIMER_LEFT_3_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_RIGHT_3_SEQUENCE=AACAACATGTTCACACTCCCAA
PRIMER_LEFT_3=100,22
PRIMER_RIGHT_3=312,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=60.312
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.3504
PRIMER_LEFT_4_PENALTY=0.037936
PRIMER_RIGHT_4_PENALTY=0.312496
PRIMER_LEFT_4_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_RIGHT_4_SEQUENCE=AAACAACATGTTCACACTCCCA
PRIMER_LEFT_4=100,22
PRIMER_RIGHT_4=313,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=60.312
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_01993 Ps_contig_mira-and-tgicl-ass_franssen_17906
SEQUENCE=TTAATTACCTATCCCCTACCCCAAACGACCACCTACCTACCTAATAGTTTTCCYTTCCTTCCTTCCTTAACCCCAAAAACTTTTAMCCYTTMCTTCCCCCAMCGTTTTTGGTAATTTCTACCCAAAACTAAAGTGACCAAAATAGCAAGAATGACTAAGAATCAGCAAGACTGAAGAAATCACCAGCCAAGTACTTTGTTTTTGATAGTCTGTCTTCATAAATATCCAACACTTTTTYYATCTTTTCATCACTCTCTTCAATCAGTTTTTGGTCAGAAGGAACACCCTTTAATGGAGCAAACAGAACTTGGACGACCAAGTTGTAGATTGCTGGGTGAAAGTTGAGCTTCAACTTCAAGCCATTGTTCCACAAGACCCCTTTCTTCTATTGTCTTTCCTAATAAATCAGTACCTTGGTTCTTGTTACTTTTCTGCATAGYATCTTATTATAGCTCGAGATTCGTAGAAGGTAAATCACCATCTTGAATAAGAGGAAGTTCTACCAAAAGGCTGCAGTTTAGGAAGGTAAAGTTCGGGTTGTTGTTGTTT
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01994 Ps_contig_mira-and-tgicl-ass_franssen_17906
SEQUENCE=TTAATTACCTATCCCCTACCCCAAACGACCACCTACCTACCTAATAGTTTTCCYTTCCTTCCTTCCTTAACCCCAAAAACTTTTAMCCYTTMCTTCCCCCAMCGTTTTTGGTAATTTCTACCCAAAACTAAAGTGACCAAAATAGCAAGAATGACTAAGAATCAGCAAGACTGAAGAAATCACCAGCCAAGTACTTTGTTTTTGATAGTCTGTCTTCATAAATATCCAACACTTTTTYYATCTTTTCATCACTCTCTTCAATCAGTTTTTGGTCAGAAGGAACACCCTTTAATGGAGCAAACAGAACTTGGACGACCAAGTTGTAGATTGCTGGGTGAAAGTTGAGCTTCAACTTCAAGCCATTGTTCCACAAGACCCCTTTCTTCTATTGTCTTTCCTAATAAATCAGTACCTTGGTTCTTGTTACTTTTCTGCATAGYATCTTATTATAGCTCGAGATTCGTAGAAGGTAAATCACCATCTTGAATAAGAGGAAGTTCTACCAAAAGGCTGCAGTTTAGGAAGGTAAAGTTCGGGTTGTTGTTGTTT
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01995 Ps_contig_mira-and-tgicl-ass_franssen_17906
SEQUENCE=TTAATTACCTATCCCCTACCCCAAACGACCACCTACCTACCTAATAGTTTTCCYTTCCTTCCTTCCTTAACCCCAAAAACTTTTAMCCYTTMCTTCCCCCAMCGTTTTTGGTAATTTCTACCCAAAACTAAAGTGACCAAAATAGCAAGAATGACTAAGAATCAGCAAGACTGAAGAAATCACCAGCCAAGTACTTTGTTTTTGATAGTCTGTCTTCATAAATATCCAACACTTTTTYYATCTTTTCATCACTCTCTTCAATCAGTTTTTGGTCAGAAGGAACACCCTTTAATGGAGCAAACAGAACTTGGACGACCAAGTTGTAGATTGCTGGGTGAAAGTTGAGCTTCAACTTCAAGCCATTGTTCCACAAGACCCCTTTCTTCTATTGTCTTTCCTAATAAATCAGTACCTTGGTTCTTGTTACTTTTCTGCATAGYATCTTATTATAGCTCGAGATTCGTAGAAGGTAAATCACCATCTTGAATAAGAGGAAGTTCTACCAAAAGGCTGCAGTTTAGGAAGGTAAAGTTCGGGTTGTTGTTGTTT
TARGET=537,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01996 Ps_contig_mira-and-tgicl-ass_franssen_17925
SEQUENCE=GATCGCCGTTGGGTAACATTTCATCTCCAGAGCGCGATCGAACTCGGAGGAGGAAGAAGAAGTGGTAGAGGTGGTGGTGCTCTTGCTTCAAAATGCAATGCTGCAGGTGAGATTTTTCAGATTGCGGCTATTATGAATGGACTTACTCTTGTTGGTGTTGCTGTTGGGTTTGTTCTTCTTCGAATTGAAGCTGCTGTTGAAGAAGCTGAGTAAGTWAGTTTTTATTATATGTATGTAATGTAATGTAAAGTTTATTAATATCTAGTTTTATTCTTGATGTAGATGATGTTAGAACTACTAAATTTAGTTTAAATTGTTTACGKTTAGGGGTAAGGGTTTAGTTTTTAGGGTAATTTTCCGGTTAACCCCCTACTTTAGTTAAATTTTTTTAAG
TARGET=233,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_01997 Ps_contig_mira-and-tgicl-ass_franssen_17939
SEQUENCE=AGGAGTAATCGGCAAATTACAAATTATTATTATTGATTTGTTACTGGAGTTATTTCCTCTGGCCATACTTCCTGCTCCTCAAGTTACTGTTATGTATATGTTGCATTGACTGATAGAAGAAATTCACCAGTTATCAAATTGCCATCTATTTAGAACTTGTAATATAGTGTTCAGCAGTCATGCATTTATCTTTCAGCCTTTTTCTGTACAAAACACCAAGCGATTAGTACTTAAAAAGATATTTGTTGAGAAACTTTTCTCTGCACAATGAAATATTTATTTAGATAAGTGCATCAGACTTATCTGTTGAATTAAAAAAAAGCATGAATATAGTTGCTTTAAACGAAT
TARGET=23,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01998 Ps_contig_mira-and-tgicl-ass_franssen_17940
SEQUENCE=AATAACTCCAGTAACAAATCAATAATAATAATTTGTAATTTGCCGATTACTCCTTAAAATTGGAATTGTAGGCCTTTTTTCACTTCCCTCTCTCCCATGAGTTCACTAATATCTGACAGTGTTATAATATATTACAAACCTAACCTTTTGGTCTCAAGATGTGGACGTAACCCTTTTCAACCTGGCATGGAAATTTGCTGCTGTATTCTTGGTCCTTTATTTTTTTCCTTTCCTTCCTTTACTTTGTTTTTACCTTTTCCCCTC
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_01999 Ps_contig_mira-and-tgicl-ass_franssen_17941
SEQUENCE=TAACTTGAGGAGCAGGAAGTATGGCCAGTTTTAGGTTTTTAAATTTTTTAACTTTCCAGTAACAAATCAATAATAATAATTTGTAATTTGCCGATTACTCCTTAAAATTGGAATGTAGGCCTTTTTTCACTTCCCTCTCTCCCATGAGTTCACTAATATCTGACAGTGTTATAATATATTTACAAACCAAACCTTTTGGTCTCAAGAATGTGGACGAACCCTTTTTCAAACCTGGCATGGAAATTTGCTGCTGATTCTTGGCCTATTTTTCTTCTCCTTTCTTTGTTTTACTTTTCCCCTCTCGAACTCTGCGTTGACTACCACTTGTCCTTCGKRGACACGACMAMCGGRCAAAACGGGG
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02000 Ps_contig_mira-and-tgicl-ass_franssen_17942
SEQUENCE=AACACTATATTACAAGTTCTAAATAGATGGTTTCTTAATTTGATAACTGGTGAATTTCTTCTATCAGTCAATGCAACATATACATAACAGTAACTTGAGGAGCAGGAAGTATGGCCAGAGGAAATAACTCCAGTAACAAATCAATAATAATAATTTGTAATTTGCCGATTACTCCTTAAAATTGGAATGTAGGCCTTTTTCACTTCCCTCTCTCCCATGAGTTCRCTAATATCTGACAGTGTTATAATATATTACAAACCTAAACCTTTTGGTCTCAAGAATGGTGGACCGTAACCCTTTTTCAAACCTGGCATGGAAATTTGCTGCTGATTTCTTGGCCTTATTTTTTTCCTTTCCTTCCTTTCTTGTTTTACTTTTCCCTCT
TARGET=143,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02001 Ps_contig_mira-and-tgicl-ass_franssen_17954
SEQUENCE=CGTTTAAGTCTACCGACAATTGTAGTANACACACTAAATTACTACAATCACGCAGTACAACAATACAGTGGTTCAGTACAAATAACTGCATGTATTTATTTATTCATATCANAGTTTATTTATATTTATACATATAATGTCCCTGCGTGTTGGAGAAAACTGAGTTAACAGTTTCGAGTGCACCAGCAGCGAAAATCATCCCTGCACCTCCCACTGAGTAAGTGCTCGTTGTTCCTGCAATGYTTGTTGCAGTTACCATCTGGGATGCATGGTCCCTTGAATGTCCCAGACAAATTCTCACTAAGTTTTTGCCTCACTCACCGCTTATTTCTTGTGCAACAAAGAGAACGAGAAAGAGGAAGGAACAAGGCAGCTACTGTCTTTCTTCTCCAGTGGTACTTAAGGTAGGTTGGTATGAATATAATTAAAMTTAATATAACTCACTTACAAATAACTACTTTAACTAATTA
TARGET=93,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02002 Ps_contig_mira-and-tgicl-ass_franssen_17959
SEQUENCE=CGGGTTATTTCCGTTATATTTKGCGTTCTCTCTTCGCTTCTGCAATCTGTGATTGTCAAGAAACGAGAGAGATGATCTATTCGCGGTTTAGAACCGTCGCCGGAAGCCTCAAGTGCGCTACAAAACCTTCTTCTTACATCTTCCACCACCACCACAACCACCTCAGCTCCGTCTGAATTCCAGCAGAATCTGCNGGACTTCGGGCCGTCGGCTTAGCGGACCGAGTCACCA
TARGET=143,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02003 Ps_contig_mira-and-tgicl-ass_franssen_17962
SEQUENCE=CGTTTACATAATCCAAAAAGACTTTATTATATCAGAAATAATTTTATTTTAAAAAAATTAGGTAATAAGCTGTAAACACAGCAGTTTGAAGCAAATTTCACAGACTYTTCCAAAAGCTCYTTTTGGAAGTTCCCAATTCAAAAGCAACTCTTCAGAACTTTTCCAAAAACCTGTTGAATAACAGCGCCCCTTTTTCCAATCAGCATCAACATCACTTCACCTGCAGAAACCAATTCAAAATTAAAAACAGCTTATCAAATAAGCACTTAAAAAGTTGGAAAATAATTAATAGAAAGAGAATATTAATATGTTACACAATGAATCAAAGGAAGCGCTCAGAAACACACTGCATTATCCAAATAAACAACTTTAAATCCATAGATTAATCATCCCACAAAATTTGGAAATAGGATCCAATAAGTTATCTCACAGAAACCAACATTCATGTATATAAACCACAAAACTAATTAATAGTAACGGAGGTTTTTTTTAAAAAATTCCAGAAGAAATTTTCAAAGTAATCTTTTCTCTGTTAACTCCAAAGAGACACAACACAGTAGCAATCCTTCTCAAAAACCAAAACTGAAACAACAATAGCAAACACAACAAGAGACACAACACAGTAAGAGATTTCTCCTAAAACACCTAAACCACAACAACAACAACAAAAGCAACTTTTTTCTCAAAAAGCCTAAAGAACGACATAAACACAACAAAGTTCTTTTCAAAGAAACAAGAACCGCAACCACACGACAAAGTTATTTTTTCTAAAAACCTTAAAAAGAAACATAACAACAACAGCGAACGACAACAACATATACACACCACTGCACTCAAAGAAGTGGATGAGGCGAAGCTAGTAACGAAGAGAAACAAAGCAGAAACGAGAGGGAAGGTAAACTTATCTTTGGTCGCCGAAAAGCACGTCACCGAACGAACTCCGGTCAGGTAGATTTCTTCACGCCTTGACTAATCCAGTGCACTTTTGTATTCTCCTGTTTACGCTGATTGAAACCCCTAACGTCCGGGTTTGAAATAACGCTCCATCACCACCTAATTTAGGGTTTTACTTTAGGGTTTTGTATCGATACGCCTCTTAGACCGCTCACGCCTTGTCGATTT
TARGET=653,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02004 Ps_contig_mira-and-tgicl-ass_franssen_17967
SEQUENCE=GTCCGGCAGTGTCCCAACCGTAGTCACCGGGGAACTCTCCTGTCAAGTTAGGTATGGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGCTTGATGGGGTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATCGGCCGG
TARGET=242,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02005 Ps_contig_mira-and-tgicl-ass_franssen_17995
SEQUENCE=AATGGTGGCAAATAGGAAAGAGTCTCAAACTTTCTTTCTTTCCAATTGGAGGCCACACCTGCATGCACTTTACTCTTCCACCATTGCTTGTAATGGAAGTAATGT
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02006 Ps_contig_mira-and-tgicl-ass_franssen_18002
SEQUENCE=AACCATATCCAAAAGCCATACCATAATAACAATAATAAACTTTAATATAGGAAAGGAAACTAAAGAAATAATTAATAGAAAAGCTTTTCAACCTTGATGACATGCAAAATGGTAGACCCGATCCCAACATCTAGAACCATTAATTTAAACTTCCGTTTTTTACCAGTTCCAGTCACACCCATTCCATTGGTTTAAGCTTCAGGATTCTCAACTGGAACTTCCTCTGCGTTGATACCACTGATTCAACTGTTTTGTTCTCAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCARTT
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02007 Ps_contig_mira-and-tgicl-ass_franssen_18062
SEQUENCE=TCGGGGATCCAAACAACAACAACACTCTCATCATCGTCTCATACTTTACCTTTCATTTCAGAGTTACAGCTACATAATCCTATTTTAGTTATAGTTAATTATTCCAGTTCGATCCATTCTTGTTAGTAGTGATCATCAGTGGCTRGAAGGANAATCAAGAACAAGTACGAGGANACTACNTCCGACAACCATNGANACAGGA
TARGET=12,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02008 Ps_contig_mira-and-tgicl-ass_franssen_18122
SEQUENCE=TCGGGGATCAACACAAACCTAAGCATTTCAACTATTTATATATATATTCACAGAGATCATATCGTATATCATAAGATGACTGGTTTATTCAGCAACATCTTTGGTGGGTACGATGGTGGAACANNGGAACTGTTGATGCATTTAACAACAGTGTGGCTATCAAGATTATGGTGAGGCTGACATCGAAACTGGTGCTACAACTTACCAAGTGGTAGG
TARGET=37,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2573
PRIMER_LEFT_PENALTY=1.253706
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=GGGATCAACACAAACCTAAGCA
PRIMER_RIGHT_SEQUENCE=CCACCAAAGATGTTGCTGAATA
PRIMER_LEFT=3,22
PRIMER_RIGHT=106,22
PRIMER_LEFT_TM=61.254
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=1.3860
PRIMER_LEFT_1_PENALTY=1.253706
PRIMER_RIGHT_1_PENALTY=0.132262
PRIMER_LEFT_1_SEQUENCE=GGGATCAACACAAACCTAAGCA
PRIMER_RIGHT_1_SEQUENCE=TGTCAGCCTCACCATAATCTTG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=61.254
PRIMER_RIGHT_1_TM=60.132
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=179
PRIMER_PAIR_PENALTY_2=1.4294
PRIMER_LEFT_2_PENALTY=1.253706
PRIMER_RIGHT_2_PENALTY=0.175676
PRIMER_LEFT_2_SEQUENCE=GGGATCAACACAAACCTAAGCA
PRIMER_RIGHT_2_SEQUENCE=TTGTAGCACCAGTTTCGATGTC
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=199,22
PRIMER_LEFT_2_TM=61.254
PRIMER_RIGHT_2_TM=60.176
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=197
PRIMER_PAIR_PENALTY_3=1.4508
PRIMER_LEFT_3_PENALTY=1.253706
PRIMER_RIGHT_3_PENALTY=0.197140
PRIMER_LEFT_3_SEQUENCE=GGGATCAACACAAACCTAAGCA
PRIMER_RIGHT_3_SEQUENCE=GTAGCACCAGTTTCGATGTCAG
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=197,22
PRIMER_LEFT_3_TM=61.254
PRIMER_RIGHT_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=1.8800
PRIMER_LEFT_4_PENALTY=1.876439
PRIMER_RIGHT_4_PENALTY=0.003589
PRIMER_LEFT_4_SEQUENCE=GGATCAACACAAACCTAAGCAT
PRIMER_RIGHT_4_SEQUENCE=CCACCAAAGATGTTGCTGAATA
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=106,22
PRIMER_LEFT_4_TM=58.124
PRIMER_RIGHT_4_TM=59.996
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_02009 Ps_contig_mira-and-tgicl-ass_franssen_18133
SEQUENCE=GTCGGGGGGATCTCCCTTAATTAGTACCTCTCTCTCTCCAAAAAGGCCAGCTCATGAGACCCCTCGGTGGAAAACGACAAATACCCTCTCTCTCACATTGAAATAAAACCCTTTCCTCTTCACAACGNTCGGGGGACGATACAACCTTCTCCTTCACCTCTCAACAACCCTAAACCCGCCGCCGTCCACATCCAAACCCTCCGTCTCTCTCCACTTACCTCCCTCAATCTTCTCCACTCACCATCACCACCGGAAAATACATAAACAACCCCACACCACCATCTTCCTCATCTCTTCCGTTTTCCACTTGAAATCATCGGTCGTACCAACAACCCTCCTCCATCCATCCTTTCCTCTTCCGCCTCACCAACATAACACAAACCCTTTTCAAATTCTTCAAACTCCTTCTCCATTCTCATACACCCAAGAGAAACCCTTTTGAATGAACATCACAAAGACTATAAAAAACTAGTCTTGGCACGACTCGCTCGTGACCCAGCCCGAGACAACTCACTCACAACAAAACTCAAACTTTCCCTCAACAATATCAAAAAATCGGATCTTCACCCGATCCAAGTAGAGCTCCAACAAGAAGATCTCTCAACTCCAGTTTCCTCCGGCACAGGTCAAGGCACTGGCGAGTATTTCACCCGACTCGAAGTGGGTCAACCCGCGAAACCCTACTACATGGTTCTCGACACAGGAAGCGACGTGAACTCGATCCAAGGCAAACCATGCTCTGAATGTTACCAACAAACTGATCTGATTTATGACCCGACATCATCTTCAACCTACGAATCATTAACCTACGAAGCACAACAGTGTCAAAAGCTAGAAGTTTCTGCTTGACGTAGCGGTAAATGTCTCTATCAAGTTAACTACGATGACGGTTCTTTCACCGTCGGTGAATACATGACTGAAACGGTGTCGTTTGGTTCTTCTGGGTCGATTAATCGGGTCGCAATCGGGTGCAGGCATGATAACGAAGGTTTATTCGTTGGTGCTGCTGGTTTACTCGGTCTCGGTGGTGGGGCCCTCTCGTTAACTTCGTAGATCAAAGCGTCGTCATTTTCGTACTGTTTAGTAGATCGTAACTCGGATAAATCGTCAACATTGGAGTTCAACTCACTGAAAGCCGTTACTCCAACAACAAACACGAGAAACCTAGGTTGGTGTTTCACATGTTTTAATACTTTCGACTTGTTTGTTGCTGCTGCAAATACTTTCGACTTATTTGTTCGATTCAATTATACATTCTTGTTAAGATCTGGTTATTGGATTCATAGCAATATAATGAGTTTGATTTGTAAATAGAATACTGCTGCCATGATCTGGTTCATATGTTGTTGGTTCACTTGTTTGGTTTGATGACTGCAGTGTGCTAATGTGCTTGAGACAGGTTTTAAAGGTTTGGATTGATCCAAGTTGGACCTTACAAGTTTGGCTTGAGTTTAGAACATGTTTGTTGCAGGTTCATAGGTTGCATCAGTTCTGCAACCAGTTTCTTGCTGTAGGTTCTGGTTTAGTAAGCTGACTTGGTGTTGATTTGTGGTGATGCCGTTAATTTGTACTCCTATCATGTTAATCCTATGTTAAGTTGTTGTACCAAGCATTGGTGAGTGAATGTTGGTTAATTAGATGATGCAAATGTTAATTGGGATTGTTAGATTTATTGAATAGGACTGTTAGATTAACATGCTTTGATATTGTGAGATTGATATTGTGTAGAAATATAGCTTTGACTGTTAATTTGTAATGTAGCTATAGTGTTTGTGTTGAGCGTGTGTGATAGCTAATGAATGTGAGAATTAGATTGAAAATAGTGTGTTGTGTACA
TARGET=28,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.4176
PRIMER_LEFT_PENALTY=2.415267
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=GGGGGATCTCCCTTAATTAGTA
PRIMER_RIGHT_SEQUENCE=GGTGAAGGAGAAGGTTGTATCG
PRIMER_LEFT=4,22
PRIMER_RIGHT=157,22
PRIMER_LEFT_TM=57.585
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=4.7000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=2.4828
PRIMER_LEFT_1_PENALTY=2.415267
PRIMER_RIGHT_1_PENALTY=0.067516
PRIMER_LEFT_1_SEQUENCE=GGGGGATCTCCCTTAATTAGTA
PRIMER_RIGHT_1_SEQUENCE=TGGAGAAGATTGAGGGAGGTAA
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=235,22
PRIMER_LEFT_1_TM=57.585
PRIMER_RIGHT_1_TM=60.068
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=4.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=2.4891
PRIMER_LEFT_2_PENALTY=2.415267
PRIMER_RIGHT_2_PENALTY=0.073866
PRIMER_LEFT_2_SEQUENCE=GGGGGATCTCCCTTAATTAGTA
PRIMER_RIGHT_2_SEQUENCE=GAAGAGATGAGGAAGATGGTGG
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=296,22
PRIMER_LEFT_2_TM=57.585
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=4.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=2.4891
PRIMER_LEFT_3_PENALTY=2.415267
PRIMER_RIGHT_3_PENALTY=0.073866
PRIMER_LEFT_3_SEQUENCE=GGGGGATCTCCCTTAATTAGTA
PRIMER_RIGHT_3_SEQUENCE=GGAAGAGATGAGGAAGATGGTG
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=297,22
PRIMER_LEFT_3_TM=57.585
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=4.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=2.5146
PRIMER_LEFT_4_PENALTY=2.415267
PRIMER_RIGHT_4_PENALTY=0.099308
PRIMER_LEFT_4_SEQUENCE=GGGGGATCTCCCTTAATTAGTA
PRIMER_RIGHT_4_SEQUENCE=GGTTTAGGGTTGTTGAGAGGTG
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=175,22
PRIMER_LEFT_4_TM=57.585
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=4.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_02010 Ps_contig_mira-and-tgicl-ass_franssen_18159
SEQUENCE=AAGACAGTAGGTATCAACGCACGAGTASTTCGGGTATATCATAATATCTCTTTTCTTTTCTTTCTTTCATTAAACATTTAGTTTCTTGTCAAAAATGGTGCTAGCTGGTAAGCTTATTACAGAACTTGGTATCAAAACACCAGCTGACAAGTTCTTCAAACTGTTTGCATCAGAACTTCATGAAGTGACAAAACCTTTGTAGGAAAAAGTAATTTCCAATCCAATTAACCCAAAGTATTTCCAATCAGTTGGGTAGAAAGACGTGGG
TARGET=49,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02011 Ps_contig_mira-and-tgicl-ass_franssen_18159
SEQUENCE=AAGACAGTAGGTATCAACGCACGAGTASTTCGGGTATATCATAATATCTCTTTTCTTTTCTTTCTTTCATTAAACATTTAGTTTCTTGTCAAAAATGGTGCTAGCTGGTAAGCTTATTACAGAACTTGGTATCAAAACACCAGCTGACAAGTTCTTCAAACTGTTTGCATCAGAACTTCATGAAGTGACAAAACCTTTGTAGGAAAAAGTAATTTCCAATCCAATTAACCCAAAGTATTTCCAATCAGTTGGGTAGAAAGACGTGGG
TARGET=57,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02012 Ps_contig_mira-and-tgicl-ass_franssen_18162
SEQUENCE=TTTTTTTTAAAGTAAGAACAAAACAAACTACTAATTAAAKTAAATYAAWTAACTAGTMRTAMYATACGTARCRTACCAACCAACCAAAATAAAAAAAGTAACAATGAATAAATGAATGTGTTACATAAGAATATATGAATAATAATAAATCATTAATAATAATAATTCATATTTCAAACAAAACCTAAAAGTGTCTTCATCCTTCATCCTATGCACAAATCAAACTCACATAGTTAAAGCATCAAAAGAGAAACCCAAATGCAAGAGCTATGAGAGAAGCAACAGCACTTGGAACAAAAAGTGCAGTAGCATCAGAAGCAGGACTAGGAGCTGGTGAATCGGCAGCTGAAACACCACTTGTAGCTATCACCAACAAAGCAACCATAACCGCCACAAAAATTCATCTTCAGTGCTTCCATTGTTTTCAAGAGAGAGAATGTTTTGAAACAAAGAAAGATTAGTGGCAGTGGGTCCCSCGTACTCTGCGTTGA
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02013 Ps_contig_mira-and-tgicl-ass_franssen_18162
SEQUENCE=TTTTTTTTAAAGTAAGAACAAAACAAACTACTAATTAAAKTAAATYAAWTAACTAGTMRTAMYATACGTARCRTACCAACCAACCAAAATAAAAAAAGTAACAATGAATAAATGAATGTGTTACATAAGAATATATGAATAATAATAAATCATTAATAATAATAATTCATATTTCAAACAAAACCTAAAAGTGTCTTCATCCTTCATCCTATGCACAAATCAAACTCACATAGTTAAAGCATCAAAAGAGAAACCCAAATGCAAGAGCTATGAGAGAAGCAACAGCACTTGGAACAAAAAGTGCAGTAGCATCAGAAGCAGGACTAGGAGCTGGTGAATCGGCAGCTGAAACACCACTTGTAGCTATCACCAACAAAGCAACCATAACCGCCACAAAAATTCATCTTCAGTGCTTCCATTGTTTTCAAGAGAGAGAATGTTTTGAAACAAAGAAAGATTAGTGGCAGTGGGTCCCSCGTACTCTGCGTTGA
TARGET=154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02014 Ps_contig_mira-and-tgicl-ass_franssen_18174
SEQUENCE=GTCATATTGTTGCTTATTATATCTTGGTTCCTTACTYTTCTTAGCAATGGTTATGATCCTAGTTTCTTTGGTTAATTAAGATTACTATGTTGTTCGTTTATGTTGTTTTAATTTGTCTTTTCTTGTCCCTTTTATGTTTATTAAGATGATGATGATGATGATGATGATGTATAGTTTATTTGTATGAGATATTCAACAAGTTAAGTTCCTTGGATTAAAGCATTTTGATTTGGATAAAAGCTTCTCTGGACTATGAAAACCTTTTTTCTATATTAGAAAAATT
TARGET=145,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02015 Ps_contig_mira-and-tgicl-ass_franssen_18190
SEQUENCE=TGTTATTTTATTTTATTTATTTATTATTTATATTATTTTATTTTTTCTTAAACTTATGCGAACAAAATTAAAATTTAATTCTAATTTTGGTGGCATACATCATATTTATTATATGAAAGCTAGTGCTGGGAATTGGAATAGAGAACAAATAAACACTAGAAAAAAACACTAACATTCAGTTGCATAACAAACATGAATAATAATTCACAATGAAGAAATACAATTAAAATGACCTCTTCAACAAAAACACAATACGACGACTCRCTTTCCGGGAACAAAGGTTTGGTGGCATATGACCACGNATTTGTTGTTAACGTGGGTAACGG
TARGET=3,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02016 Ps_contig_mira-and-tgicl-ass_franssen_18190
SEQUENCE=TGTTATTTTATTTTATTTATTTATTATTTATATTATTTTATTTTTTCTTAAACTTATGCGAACAAAATTAAAATTTAATTCTAATTTTGGTGGCATACATCATATTTATTATATGAAAGCTAGTGCTGGGAATTGGAATAGAGAACAAATAAACACTAGAAAAAAACACTAACATTCAGTTGCATAACAAACATGAATAATAATTCACAATGAAGAAATACAATTAAAATGACCTCTTCAACAAAAACACAATACGACGACTCRCTTTCCGGGAACAAAGGTTTGGTGGCATATGACCACGNATTTGTTGTTAACGTGGGTAACGG
TARGET=12,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02017 Ps_contig_mira-and-tgicl-ass_franssen_18199
SEQUENCE=AGTACGCGGGGGTACCTACCCCAAACCCATTTCCTCAGTACCGGTTGTTCCTTCAACAACCTCAACTTTCTCAACTGGTCYACCGAAGTGTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTTCTCACCATCTTCTCCTTCCTCTTCACTTGACTATACATGCACACATAAAAAAWWACACTGATTATTTTTCAATACTAATAATATATTTTGAATAATACTAACAATGTTAGTTACACACATAAAACGAATCAAAACGATTAGGGCTCAATTACTTACGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGGTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCACCTTGTGACCAAACTCGTGGGCAATCGCCTCATCTTGAGCAGGCTTATGTTCTTCATCCTTTTTCTTACCACCGAGAAAATCAAAAACRCCTCTGTCCTTGATCTCTGTCTCAGAGTTGGTTGCGGGAGGTTACGTCTCCTCGTACTTGTTCTGATTCTCCTCAGCCATGATGATCACTACTAACAAGATGGATCGAGCTAGGAAATTAACTGTAAACTAAATGGATTATGTAGCTAGTAACTCTAGAAATAGGAAAAGGTTAAG
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02018 Ps_contig_mira-and-tgicl-ass_franssen_18231
SEQUENCE=AAGCAGTGGATCAACGCAGAGTTGTTTTGGTTAAGTTTGTAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGGTTGTAAGATTACAGTATCTCTTATGTGTTGTGAGTTTCTTTTTTTACTGTGTACTACTTT
TARGET=186,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02019 Ps_contig_mira-and-tgicl-ass_franssen_18236
SEQUENCE=TAGTGTCCACCGCCATATTTGGCATCATTTACTTCATTTGACTTTTCAACAACCTCTGCAAATACAACCGTGTAATATATTAGATAACCACAAAAGATTCATGTCTGTCTATCTATACCTCTTTTATTTGACACTCAACATTATTTATTCATGTTACTTATTACTCTTAAAATGTCCATGCATTATGATATAATCAGTTACCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCCGTAACCACCACCACCGCCATGAMCCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATCG
TARGET=254,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02020 Ps_contig_mira-and-tgicl-ass_franssen_18236
SEQUENCE=TAGTGTCCACCGCCATATTTGGCATCATTTACTTCATTTGACTTTTCAACAACCTCTGCAAATACAACCGTGTAATATATTAGATAACCACAAAAGATTCATGTCTGTCTATCTATACCTCTTTTATTTGACACTCAACATTATTTATTCATGTTACTTATTACTCTTAAAATGTCCATGCATTATGATATAATCAGTTACCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCCGTAACCACCACCACCGCCATGAMCCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATCG
TARGET=279,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02021 Ps_contig_mira-and-tgicl-ass_franssen_18238
SEQUENCE=TTGTCGGAAGCACCACCATGTCCACCATGATGGCCACCACCATGACCGTAGTGACCACCACCATAGTGTCCACCTCCATAGTGTCCACCACCATAGTGTCCACCGCCATATTTGGCATCATTTACTTCATTTGACTTTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCATGACCGTAGCCTCCATGACCGTAGCCTCCATGTCCATGA
TARGET=201,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3084
PRIMER_LEFT_PENALTY=0.265715
PRIMER_RIGHT_PENALTY=2.042732
PRIMER_LEFT_SEQUENCE=CTCCATAGTGTCCACCACCATA
PRIMER_RIGHT_SEQUENCE=GACATGGAGGCTACGGTCAT
PRIMER_LEFT=73,22
PRIMER_RIGHT=246,20
PRIMER_LEFT_TM=59.734
PRIMER_RIGHT_TM=59.957
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=2.3084
PRIMER_LEFT_1_PENALTY=0.265715
PRIMER_RIGHT_1_PENALTY=2.042732
PRIMER_LEFT_1_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_RIGHT_1_SEQUENCE=GACATGGAGGCTACGGTCAT
PRIMER_LEFT_1=58,22
PRIMER_RIGHT_1=246,20
PRIMER_LEFT_1_TM=59.734
PRIMER_RIGHT_1_TM=59.957
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=2.3387
PRIMER_LEFT_2_PENALTY=0.295944
PRIMER_RIGHT_2_PENALTY=2.042732
PRIMER_LEFT_2_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_RIGHT_2_SEQUENCE=GACATGGAGGCTACGGTCAT
PRIMER_LEFT_2=64,22
PRIMER_RIGHT_2=246,20
PRIMER_LEFT_2_TM=60.296
PRIMER_RIGHT_2_TM=59.957
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=2.5728
PRIMER_LEFT_3_PENALTY=0.295944
PRIMER_RIGHT_3_PENALTY=2.276885
PRIMER_LEFT_3_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_RIGHT_3_SEQUENCE=GCTACGGTCATGGAGGCTAC
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=237,20
PRIMER_LEFT_3_TM=60.296
PRIMER_RIGHT_3_TM=59.723
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=2.6646
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=2.042732
PRIMER_LEFT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=GACATGGAGGCTACGGTCAT
PRIMER_LEFT_4=139,22
PRIMER_RIGHT_4=246,20
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=59.957
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_02022 Ps_contig_mira-and-tgicl-ass_franssen_18259
SEQUENCE=CGGTAGAATAGTAACAAAAAGGAACCATATCATTATTAACTCTTATCCATCCATTTGTCATTTTGTATGTACCGAAAAACAAAAAMTGAAAGAACACAGTAAATTACAAGCAGAACAAATGATAAGAAAGAAAACAGTCTTTTCCAATGCCAATTAAATACCTACTAAACGTTAGGTAGGATTCTGGTGGTGGTGGGCAATAGAAACTGATGCATTAG
TARGET=185,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02023 Ps_contig_mira-and-tgicl-ass_franssen_18265
SEQUENCE=TCGGGGTATKTCSATTCCATTCCATTCCATTCACTCATCAAACCAAAAACAATGGCAGCATCAGCATCATCCATGATCTCAACAAACGCCCTAACATCCACACTCCAAAAACACCAAACACACAACCTCAAACCATCAAACCTATGTTTCCAAGGCTTCAAACCCTTAACAAGATCAACAAAACTGCACAGAAACAAAAAGAGATTCTCATYCCTTGTTGTACAAAGACTGAACTACCAACCCTTMTA
TARGET=14,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02024 Ps_contig_mira-and-tgicl-ass_franssen_18267
SEQUENCE=CTAAGTTTAGCAACCAAGTACAACAAGGCAGTTTGTTCTTAAGTAGGTAAGATGAGCTGCCCCCAGAAAAGCTTGCAATTGCTAATGTGGGAAGGCAGGATGCTAAGAAGCATCTTGAAGAGCACGTTTCTAATTTAATGTCTTCGAACATTGTTCAGACTTTGGGAATGATGCTCGACACAGTTGTGTTCTAGAGTTAGGATCGTCCACTGTATTCTTCTTCTTCYTCATGATGTCCAGCAATATTTCATTTACACTTCTTTGACCTTGTTTGCAATTAGTATTTTGTTTTTTAATTAACTAAAGTTTATTTGCTATTTCATTGTGTTTCCGTCTACTAAA
TARGET=215,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02025 Ps_contig_mira-and-tgicl-ass_franssen_18287
SEQUENCE=TGTTGGAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTCACTCACTGCTTGTCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGTAGTTTCTTTTTTTACTGTGTACACTTTA
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02026 Ps_contig_mira-and-tgicl-ass_franssen_18298
SEQUENCE=CCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAAGCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGAAGGCATAAATGTGAACGATTTTGTTCCTATAAAGACAAGACTCAATGAAATTGGCCGGCGTTATTTAAAAAGGATCATCAAGAAGATGCTAACATTCAATTATGGGTTAATCAATGTTTGGTGGCAGCCAAAGGTTCGTGGGAGCATGATGTTTATCCTTAATTTTAGTTTCCTATTATTCAGTTTTTTCGCTTTTCTTTTGTACTTTTTTGTTATGTTGGGTGTTGTTTTAGTTCTAAATACCTTGTTTACTATAATTGTGTCTCTATTGTCTTGTTTATACTCTTTCTATTTTCCTCTTTCATATGTATTGTTTGTACTCTTTCTATTTCCCTYTCACATTTTGTGTTAGAAGGAATTTATATGAATAAAATAGTGATGTTTGMWACT
TARGET=82,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02027 Ps_contig_mira-and-tgicl-ass_franssen_18320
SEQUENCE=GGAGGAGTAAAACGTAGAAACTTTTAGTGCCAGAGTTTTGGGAGAGTGATATGGCACAGTGGTCGTTGCTTATCGTTTTGTTCTGCGTCGCCTCCGCCGCCGCCGGATTCAGCTTCCATGACTCCAATCCTATTCGAATGGTCTCCCAACGAAATCTCATGGATTAATCTCTTCCTTAACTCAATACCCTCTTTCAAGAAGCGTGCAGAGAGCGA
TARGET=94,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1871
PRIMER_LEFT_PENALTY=0.155310
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=CTTTTAGTGCCAGAGTTTTGGG
PRIMER_RIGHT_SEQUENCE=ATTGGAGTCATGGAAGCTGAAT
PRIMER_LEFT=20,22
PRIMER_RIGHT=127,22
PRIMER_LEFT_TM=60.155
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=108
PRIMER_PAIR_PENALTY_1=0.2784
PRIMER_LEFT_1_PENALTY=0.061818
PRIMER_RIGHT_1_PENALTY=0.216588
PRIMER_LEFT_1_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_1_SEQUENCE=CACGCTTCTTGAAAGAGGGTAT
PRIMER_LEFT_1=57,22
PRIMER_RIGHT_1=204,22
PRIMER_LEFT_1_TM=60.062
PRIMER_RIGHT_1_TM=59.783
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=148
PRIMER_PAIR_PENALTY_2=0.3111
PRIMER_LEFT_2_PENALTY=0.061818
PRIMER_RIGHT_2_PENALTY=0.249232
PRIMER_LEFT_2_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_2_SEQUENCE=CGCTTCTTGAAAGAGGGTATTG
PRIMER_LEFT_2=57,22
PRIMER_RIGHT_2=202,22
PRIMER_LEFT_2_TM=60.062
PRIMER_RIGHT_2_TM=60.249
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.3566
PRIMER_LEFT_3_PENALTY=0.155310
PRIMER_RIGHT_3_PENALTY=0.201288
PRIMER_LEFT_3_SEQUENCE=CTTTTAGTGCCAGAGTTTTGGG
PRIMER_RIGHT_3_SEQUENCE=GACCATTCGAATAGGATTGGAG
PRIMER_LEFT_3=20,22
PRIMER_RIGHT_3=142,22
PRIMER_LEFT_3_TM=60.155
PRIMER_RIGHT_3_TM=59.799
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=123
PRIMER_PAIR_PENALTY_4=0.3566
PRIMER_LEFT_4_PENALTY=0.155310
PRIMER_RIGHT_4_PENALTY=0.201288
PRIMER_LEFT_4_SEQUENCE=CTTTTAGTGCCAGAGTTTTGGG
PRIMER_RIGHT_4_SEQUENCE=CCATTCGAATAGGATTGGAGTC
PRIMER_LEFT_4=20,22
PRIMER_RIGHT_4=140,22
PRIMER_LEFT_4_TM=60.155
PRIMER_RIGHT_4_TM=59.799
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_02028 Ps_contig_mira-and-tgicl-ass_franssen_18332
SEQUENCE=GATTCTTCTCACTATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCAACCCCAAAACATGGAAACAACCCTCCACTCTATCTTTCATCTCTCTCAAACCAACCAAGTTCACACCAAGAACAACAAAAATTGCTGCAACAGAAGACAAAACCGATGCAGCAACCGATGTAGCAAACAAAAGAAAGACAACCAGTTGGATTCACCCCACCTGAGCTAG
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02029 Ps_contig_mira-and-tgicl-ass_franssen_18333
SEQUENCE=AACTCTGGGCAATCGCCTCATCTTGTTAGTCAGGCTTATGTTCTTCATCCTTTTTCTTACCACCGAGAAAATCAAAAAACACCTCTGTCCTTGATCTCTGTCTCAGAGTTGGTTGCGGAGGTAGTCTCCTCGTACTTGTTCTGATTCTCCTCAGCCATGATGATCACTACTAACAAGATGGATCGAACTAGGAAATTAATTAACTGTAAACTAAAATGGATTATGTAGCTGTAACTCTAGAAATAGGAAAAGGTAAAGTATGGAMGACGATGAYGATGTTGTTGTTGTTGTTGNTCGACCTCCCG
TARGET=277,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02030 Ps_contig_mira-and-tgicl-ass_franssen_18337
SEQUENCE=CGGACACCACCCAACTGTCCTACCCACTGATGACCACCACCACTNACCGTAGTGACCACCACCACTGCACACCTACCCTCCATCCTCACCCTACCGTCTTACCACTCCCACCACCACCAYTCGCACAYCACCCTCACCCTCACCCTCGTCTACCACTCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGAGTTCTTCACATATATATTTCGTTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCAGCTGTTTGCATGAATTGTAATTTCAAAGTTCGGTTTNANCAAGCAAGCCAAATAAATNTATCCTACTAAAAATT
TARGET=108,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02031 Ps_contig_mira-and-tgicl-ass_franssen_18337
SEQUENCE=CGGACACCACCCAACTGTCCTACCCACTGATGACCACCACCACTNACCGTAGTGACCACCACCACTGCACACCTACCCTCCATCCTCACCCTACCGTCTTACCACTCCCACCACCACCAYTCGCACAYCACCCTCACCCTCACCCTCGTCTACCACTCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGAGTTCTTCACATATATATTTCGTTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCAGCTGTTTGCATGAATTGTAATTTCAAAGTTCGGTTTNANCAAGCAAGCCAAATAAATNTATCCTACTAAAAATT
TARGET=159,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02032 Ps_contig_mira-and-tgicl-ass_franssen_18337
SEQUENCE=CGGACACCACCCAACTGTCCTACCCACTGATGACCACCACCACTNACCGTAGTGACCACCACCACTGCACACCTACCCTCCATCCTCACCCTACCGTCTTACCACTCCCACCACCACCAYTCGCACAYCACCCTCACCCTCACCCTCGTCTACCACTCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGAGTTCTTCACATATATATTTCGTTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCAGCTGTTTGCATGAATTGTAATTTCAAAGTTCGGTTTNANCAAGCAAGCCAAATAAATNTATCCTACTAAAAATT
TARGET=205,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02033 Ps_contig_mira-and-tgicl-ass_franssen_18357
SEQUENCE=CGAACAAATTTTAAAGTAAACCAAAACTAAACAATAATTAACCAATTAAATTTACTAAGAAAAGGAAAAATAACAACCCAACCAACCAAAGGGACCGTTAAACTTAGATAGTAATTTAAATTTAGTAACAAAAACAAACCCCCCAAACGACCACAACCAACAACAACCAAATACGATCATATTCAATGAGAAGTAGTCTCATCCTTGTGATGATCTTTGTTTCTTTCACCATCAGTAGCAGTCTTAGCATGATAACCAGGAAGCTTCTCTTTGATCTTTTCCAAAATACCTTTCTTCTCTCCCTCATGAATAGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTTGTTACCTTCCTCTTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTATTTGCAAGGAACCTTCTTTTCCTCGTAACGGTTCCCGGTTTGTTTCCCTTACC
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02034 Ps_contig_mira-and-tgicl-ass_franssen_18390
SEQUENCE=GTCGGGAGTGTCTGCACTTCTCAACCACGGAAATGGCTTCCATGGCTTTCTCCACAACCTTCTTCACTCCTCWKCRKGAWKTCGGGGAAACCACACACACATCATGGGTTTGCAGGAGGATTTTGAGGAGCATGCTGCGAAAGTTAAGACTCTAACAGAGAGTCCATCAAATGAAAACTTGCTTATCCTTTATGGATTGTACAAGCAAGCCACTCTTGGACCTGTTACCACCGCTCGTCCTGGGATTTTCAGCCAGAAAGACAGAGACTAAAGTGGGATGCAGTGGGAAGGCTTGGTTAGGAAAGGAAAAYWCCGAAAGGTAAG
TARGET=92,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02035 Ps_contig_mira-and-tgicl-ass_franssen_18393
SEQUENCE=TGCAAAATGGTAGACCCGATCCCAACATCTTAGAACCATTAATTTAAACTTCCGTTTTTTACCAGTTCCAGTCACACCCATTCCATTGGTTTAAGCTTCAGGATTCTCAACTGGAACTTCCTCTGCTGCTGGAGCTTCCACTGATTCAACTGTTTTGTTCTCAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCTATTTTCAGTTAGTTGCTATTGGCCTCCTCCACTACTGTCCTTCCGCCTTCCTTTCCCTTCCTACCTTTCCTTACCAACCGTACTTTCCCTCTAGTTTTCTTTAGGTCTCCTTTACTTAACGTTTCCTTCCTTTCCTTTCG
TARGET=184,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0386
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=ACACCCATTCCATTGGTTTAAG
PRIMER_RIGHT_SEQUENCE=ACAGTAGTGGAGGAGGCCAATA
PRIMER_LEFT=73,22
PRIMER_RIGHT=242,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.0716
PRIMER_LEFT_1_PENALTY=0.052729
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=CTCAACTGGAACTTCCTCTGCT
PRIMER_RIGHT_1_SEQUENCE=ACAGTAGTGGAGGAGGCCAATA
PRIMER_LEFT_1=105,22
PRIMER_RIGHT_1=242,22
PRIMER_LEFT_1_TM=60.053
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=0.0743
PRIMER_LEFT_2_PENALTY=0.019805
PRIMER_RIGHT_2_PENALTY=0.054491
PRIMER_LEFT_2_SEQUENCE=ACACCCATTCCATTGGTTTAAG
PRIMER_RIGHT_2_SEQUENCE=GAAAGGTAGGAAGGGAAAGGAA
PRIMER_LEFT_2=73,22
PRIMER_RIGHT_2=273,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=59.946
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.1333
PRIMER_LEFT_3_PENALTY=0.114470
PRIMER_RIGHT_3_PENALTY=0.018835
PRIMER_LEFT_3_SEQUENCE=AAGCTTCAGGATTCTCAACTGG
PRIMER_RIGHT_3_SEQUENCE=ACAGTAGTGGAGGAGGCCAATA
PRIMER_LEFT_3=92,22
PRIMER_RIGHT_3=242,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=60.019
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.1638
PRIMER_LEFT_4_PENALTY=0.019805
PRIMER_RIGHT_4_PENALTY=0.143968
PRIMER_LEFT_4_SEQUENCE=ACACCCATTCCATTGGTTTAAG
PRIMER_RIGHT_4_SEQUENCE=CAGTAGTGGAGGAGGCCAATAG
PRIMER_LEFT_4=73,22
PRIMER_RIGHT_4=241,22
PRIMER_LEFT_4_TM=59.980
PRIMER_RIGHT_4_TM=60.144
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=169
=
PRIMER_SEQUENCE_ID=SSR_02036 Ps_contig_mira-and-tgicl-ass_franssen_18396
SEQUENCE=GATCTTTAAAAATTATCACTCTTAACTAGTCACATTACATCCTTTTCTTTTATGATGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAAAACCACCTGTCACAATACCACCTATCACAGTCCCTCCCGTTGTACCTCTTCCACCTCTTCCAGTACCTCCCGTCACCGTTCCTCCGGTTGTACCTCTTCCAACATGTTCCAATCCCTCCAGTTGTACCTCTTCC
TARGET=164,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4030
PRIMER_LEFT_PENALTY=0.177008
PRIMER_RIGHT_PENALTY=0.225996
PRIMER_LEFT_SEQUENCE=TTTATGATGATGAACGGTCAGG
PRIMER_RIGHT_SEQUENCE=TAGGTGGTATTGTGACAGGTGG
PRIMER_LEFT=48,22
PRIMER_RIGHT=259,22
PRIMER_LEFT_TM=59.823
PRIMER_RIGHT_TM=59.774
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.4237
PRIMER_LEFT_1_PENALTY=0.177008
PRIMER_RIGHT_1_PENALTY=0.246726
PRIMER_LEFT_1_SEQUENCE=TTTATGATGATGAACGGTCAGG
PRIMER_RIGHT_1_SEQUENCE=GGAGGGACTGTGATAGGTGGTA
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=59.823
PRIMER_RIGHT_1_TM=60.247
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=0.5002
PRIMER_LEFT_2_PENALTY=0.177008
PRIMER_RIGHT_2_PENALTY=0.323242
PRIMER_LEFT_2_SEQUENCE=TTTATGATGATGAACGGTCAGG
PRIMER_RIGHT_2_SEQUENCE=AAGAGGTGGAAGAGGTACAACG
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=59.823
PRIMER_RIGHT_2_TM=59.677
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.5249
PRIMER_LEFT_3_PENALTY=0.298898
PRIMER_RIGHT_3_PENALTY=0.225996
PRIMER_LEFT_3_SEQUENCE=TGGATGGACGTAACATCACAGT
PRIMER_RIGHT_3_SEQUENCE=TAGGTGGTATTGTGACAGGTGG
PRIMER_LEFT_3=73,22
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=60.299
PRIMER_RIGHT_3_TM=59.774
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.5366
PRIMER_LEFT_4_PENALTY=0.298898
PRIMER_RIGHT_4_PENALTY=0.237699
PRIMER_LEFT_4_SEQUENCE=TGGATGGACGTAACATCACAGT
PRIMER_RIGHT_4_SEQUENCE=CAGGTGGTTTTACCGTATCCTC
PRIMER_LEFT_4=73,22
PRIMER_RIGHT_4=244,22
PRIMER_LEFT_4_TM=60.299
PRIMER_RIGHT_4_TM=59.762
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_02037 Ps_contig_mira-and-tgicl-ass_franssen_18396
SEQUENCE=GATCTTTAAAAATTATCACTCTTAACTAGTCACATTACATCCTTTTCTTTTATGATGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAAAACCACCTGTCACAATACCACCTATCACAGTCCCTCCCGTTGTACCTCTTCCACCTCTTCCAGTACCTCCCGTCACCGTTCCTCCGGTTGTACCTCTTCCAACATGTTCCAATCCCTCCAGTTGTACCTCTTCC
TARGET=217,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4030
PRIMER_LEFT_PENALTY=0.177008
PRIMER_RIGHT_PENALTY=0.225996
PRIMER_LEFT_SEQUENCE=TTTATGATGATGAACGGTCAGG
PRIMER_RIGHT_SEQUENCE=TAGGTGGTATTGTGACAGGTGG
PRIMER_LEFT=48,22
PRIMER_RIGHT=259,22
PRIMER_LEFT_TM=59.823
PRIMER_RIGHT_TM=59.774
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.4237
PRIMER_LEFT_1_PENALTY=0.177008
PRIMER_RIGHT_1_PENALTY=0.246726
PRIMER_LEFT_1_SEQUENCE=TTTATGATGATGAACGGTCAGG
PRIMER_RIGHT_1_SEQUENCE=GGAGGGACTGTGATAGGTGGTA
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=59.823
PRIMER_RIGHT_1_TM=60.247
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=0.5002
PRIMER_LEFT_2_PENALTY=0.177008
PRIMER_RIGHT_2_PENALTY=0.323242
PRIMER_LEFT_2_SEQUENCE=TTTATGATGATGAACGGTCAGG
PRIMER_RIGHT_2_SEQUENCE=AAGAGGTGGAAGAGGTACAACG
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=59.823
PRIMER_RIGHT_2_TM=59.677
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.5249
PRIMER_LEFT_3_PENALTY=0.298898
PRIMER_RIGHT_3_PENALTY=0.225996
PRIMER_LEFT_3_SEQUENCE=TGGATGGACGTAACATCACAGT
PRIMER_RIGHT_3_SEQUENCE=TAGGTGGTATTGTGACAGGTGG
PRIMER_LEFT_3=73,22
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=60.299
PRIMER_RIGHT_3_TM=59.774
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.5612
PRIMER_LEFT_4_PENALTY=0.177008
PRIMER_RIGHT_4_PENALTY=0.384229
PRIMER_LEFT_4_SEQUENCE=TTTATGATGATGAACGGTCAGG
PRIMER_RIGHT_4_SEQUENCE=GGAAGAGGTGGAAGAGGTACAA
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=296,22
PRIMER_LEFT_4_TM=59.823
PRIMER_RIGHT_4_TM=59.616
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_02038 Ps_contig_mira-and-tgicl-ass_franssen_18401
SEQUENCE=TGGTATCAACGCAGAGTGTGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAATTGAGGATACGTTCTTCAATGGCGAAACAGAGAAATCTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACNGTGTACACTT
TARGET=173,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02039 Ps_contig_mira-and-tgicl-ass_franssen_18404
SEQUENCE=GACCACCACATCTTTTSATACGATATCATATAATTCCATAACCAACCTTCTCTTCCATTCCATTCCATTCCATTCACTCATCAAACCAAAAACAATGGCAGCATCAGCATCATCCATGATCTCAACAAACGCCCTAACATCCACACTCCAAAACACCAACACACAACCTCAAACCATCAAACCTATGTTTCCAAGGCTTCAAACCCTTAACAAGATCAACAAAACTGRCACAGAACAAAAAGAAGATTCTCATCCTTCGTTGTCAAGACTGAACTCACCTTCTA
TARGET=53,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02040 Ps_contig_mira-and-tgicl-ass_franssen_18414
SEQUENCE=ATCAACGCAGAGTWCGCGGGAAAACAAGAAAGCAGATAACAGAAAAAAAGGTAGTGAGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGCAGCAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTGCTCTGCTTTGCCTTATCTCCCTCCTCCTCGCCTCCTCCACCACCACCTTCCTTTCCTTTCCTTTCCCCCTTTACCCTAAACTATTTTTCAGAGTCGGCGGTAAGTTTTCTCTTCATCGGACTAGAGTCTTTCTTCTT
TARGET=56,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02041 Ps_contig_mira-and-tgicl-ass_franssen_18414
SEQUENCE=ATCAACGCAGAGTWCGCGGGAAAACAAGAAAGCAGATAACAGAAAAAAAGGTAGTGAGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGCAGCAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTGCTCTGCTTTGCCTTATCTCCCTCCTCCTCGCCTCCTCCACCACCACCTTCCTTTCCTTTCCTTTCCCCCTTTACCCTAAACTATTTTTCAGAGTCGGCGGTAAGTTTTCTCTTCATCGGACTAGAGTCTTTCTTCTT
TARGET=202,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02042 Ps_contig_mira-and-tgicl-ass_franssen_18416
SEQUENCE=CGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCTCCTCCACCTTACCACTACTAAATAGTGGTTTACGGTAGATCCCTTGGATTTTAGGTATATATTGGAAATAAGCAGGAAGATGTCATTGGCAATAATACAAGGAAGCAAAGTTTCAACGTAGTTTGTTTCAAGAAAAGCCACTCTTTTTATCTCATGTGTTTTGCTATTCTCATTGTTAAAAAGTTTAAGTTTAGGTTTTTGAACTTAGACAGTTCTAAGGCATGTGTGTAATGTATTTTGCAACCTACTCGTTTGAAGGGATCACCAAAATTAAATAAAATATTACGTTCTACTATTCTATTTTTTCTAAG
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02043 Ps_contig_mira-and-tgicl-ass_franssen_18423
SEQUENCE=AAGACAGTGGTATCAACGCAGAGTCTCCTTCACAGGGTTTTCTTTTGTACTCTTATTAGCATCCATGATGTTCAATTAGGAAAATCTTACTGTATTTAATTTATCTATGTAATTGGCTTGGCTACTGAGTTAGTATTTGATTGCGTGTACTTTCTTTTTCATTATTGTGTTAATAAAATTAATTAATTTATTGTC
TARGET=177,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02044 Ps_contig_mira-and-tgicl-ass_franssen_18436
SEQUENCE=GKAATGCCTATATATAGAAAGCTTTCCTATGCTCATMRGTGACATACACCAACATACATACATATCTTCTTCTCAAACTCCTTTTTATCAAATCAATAATCATGGGTGTTTTCACATATGAGAATGATACCACCTCTACCGTCCCTCCTGCCAAGCTCTTCAAAGCTGTCGTGCATGACGCTGATCTCATCCGTACCCAAAAGTTGTTGATTCAATCAAGACTTGTTAGAAACTTCCTTAGGAAAGGTAAWTGGGTSGGTCCAGGYTACSCGAACGGGG
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02045 Ps_contig_mira-and-tgicl-ass_franssen_18442
SEQUENCE=TCGGGTCGAGGTTTCAGCTAGAGAATTAGCTGAGACTTCCACTAATGCCAAAGAGGAAGTTGCTGAAAAGTCAAATGAAGTAAATGATGCCAAAATATTACGGGTGGAGGTTATGGTCGTGGCTAGTGGTCATGGACATGGAGGCTAACGGTCGATGGGAGGCTACGGTCAATGGGTGGTTRGGAGGTTACGGTCATGGGTGGTGGAGGTTACGGTCATGGGCGGTGGTGGTGGTTACGGCA
TARGET=224,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02046 Ps_contig_mira-and-tgicl-ass_franssen_18456
SEQUENCE=GTATTCTAGAAAATAAWWTTAAWTTTWGTCATTACAACAATAATAAACAATGATAAGGCCCGATCATCATGACATGACCGACACATTTTCCTATACAAAGGAAGCATCAAGAAAAGCAAACATGCATGCTTGTATAGGTTACATGCATACCATTACAAACATCACGCCAATTTTAATTAAACATTCTTTATTCATCATATAATGCCTTAATGATTAATTTTTGATAAGATGACCATCAATATCTCTAGTGCATTGGCTAAGATAGTCCATCCATCCATTTGGAGGATGAATATCCTCATTGATTTTCTCATATTCAACAGTCCATTTAGCAACAGCAGTGCCATCACCCTTATCAACTACTTCAAGGGTGACTTTAAAGAGCTTATAGTGCTTCTCAATGTCTCCACCAAAAAGCTTGAGAGTCAGTTTTTTATTTTGTTCATCAACTTCTTCAAAACTCTCATAACATGTGTGTACCTCACCACCTATGACATAAGTCCAGTTGTTTAACTGTATCAGTGTCATGCCAGTCTTCACCTTCATGCAGCTTGCCTTTATGAACTTCTTTACAACTAAATTGTTTTGCTACTTACGGAAATTCTAATTAGTTAAAATTAGGTTTAGTAAAGTCTTT
TARGET=266,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02047 Ps_contig_mira-and-tgicl-ass_franssen_18458
SEQUENCE=TTAAACGACGCAACAAGAAACAACAACAACTAAACAAAATAACAACAACAAACTAAAACGAAAAACAAGTAAGTAAATTACCTCGAATCCTCCACCTCTTTCATGGATAACAGCATAGTTTAACCACTACCTCCAAGAACACCCTTAAGCTTCTTCACTAAACCACGCATCAAGAAAAGTAGTTGCAGTAATCAATTCAGTAGGAAAATCACTCTTAAACAAAGCGAAATCCAATATCWGCAAACCAGGATTAGCACTACTAAAGCTAACAAAAGCAAGTGCATTAGAACGACCACTGTTAATCTAGAAAATGCAACTAACCCTTGTAGGAAAAACCATAACATCTACCTTTTAGTTTAAGGTTAGTTTTAAAGGTAAAACAACGTTAGCAGATAGAAAAA
TARGET=19,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02048 Ps_contig_mira-and-tgicl-ass_franssen_18461
SEQUENCE=ACGGTTAACGGTTAGTAAAATTACGTTCGTACTTCGTAAGATCATTAACGATCGTGAGACTGGAAGRTCAAGAGGTTTTGGATTCGTGACCTTTGCCACGGAGCAATCCATGAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGTGGTGSTKATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGT
TARGET=237,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02049 Ps_contig_mira-and-tgicl-ass_franssen_18461
SEQUENCE=ACGGTTAACGGTTAGTAAAATTACGTTCGTACTTCGTAAGATCATTAACGATCGTGAGACTGGAAGRTCAAGAGGTTTTGGATTCGTGACCTTTGCCACGGAGCAATCCATGAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGTGGTGSTKATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGT
TARGET=329,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02050 Ps_contig_mira-and-tgicl-ass_franssen_18463
SEQUENCE=TGTGATGAGATTATACTTGTAAACCGTTGTGGTGAGATGAGWCTTATAGTGGTTTTTTTACCGATGGTGGTGTGATTTATATTTATACACCTGTTGATGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAAWAYTTGTTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGACTTATATTTGTAAATCGGTGGTGGAGGAGAWGAATATATATTGATTTGGTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAAGGTTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTTGCTTGAGATGGCAAGGTGAGAGAGAGTATTGTCAATGCAATAGTGAGAGTAATAGAGGTCATTTGAGACCCCATGTTTTCTCAAAATAGGTTTTGTTGTTDCCCCCCGTACTCYSYCGA
TARGET=158,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02051 Ps_contig_mira-and-tgicl-ass_franssen_18463
SEQUENCE=TGTGATGAGATTATACTTGTAAACCGTTGTGGTGAGATGAGWCTTATAGTGGTTTTTTTACCGATGGTGGTGTGATTTATATTTATACACCTGTTGATGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAAWAYTTGTTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGACTTATATTTGTAAATCGGTGGTGGAGGAGAWGAATATATATTGATTTGGTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAAGGTTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTTGCTTGAGATGGCAAGGTGAGAGAGAGTATTGTCAATGCAATAGTGAGAGTAATAGAGGTCATTTGAGACCCCATGTTTTCTCAAAATAGGTTTTGTTGTTDCCCCCCGTACTCYSYCGA
TARGET=209,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02052 Ps_contig_mira-and-tgicl-ass_franssen_18463
SEQUENCE=TGTGATGAGATTATACTTGTAAACCGTTGTGGTGAGATGAGWCTTATAGTGGTTTTTTTACCGATGGTGGTGTGATTTATATTTATACACCTGTTGATGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAAWAYTTGTTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGACTTATATTTGTAAATCGGTGGTGGAGGAGAWGAATATATATTGATTTGGTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAAGGTTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTTGCTTGAGATGGCAAGGTGAGAGAGAGTATTGTCAATGCAATAGTGAGAGTAATAGAGGTCATTTGAGACCCCATGTTTTCTCAAAATAGGTTTTGTTGTTDCCCCCCGTACTCYSYCGA
TARGET=274,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02053 Ps_contig_mira-and-tgicl-ass_franssen_18463
SEQUENCE=TGTGATGAGATTATACTTGTAAACCGTTGTGGTGAGATGAGWCTTATAGTGGTTTTTTTACCGATGGTGGTGTGATTTATATTTATACACCTGTTGATGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAAWAYTTGTTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGACTTATATTTGTAAATCGGTGGTGGAGGAGAWGAATATATATTGATTTGGTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAAGGTTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTTGCTTGAGATGGCAAGGTGAGAGAGAGTATTGTCAATGCAATAGTGAGAGTAATAGAGGTCATTTGAGACCCCATGTTTTCTCAAAATAGGTTTTGTTGTTDCCCCCCGTACTCYSYCGA
TARGET=325,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02054 Ps_contig_mira-and-tgicl-ass_franssen_18463
SEQUENCE=TGTGATGAGATTATACTTGTAAACCGTTGTGGTGAGATGAGWCTTATAGTGGTTTTTTTACCGATGGTGGTGTGATTTATATTTATACACCTGTTGATGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAAWAYTTGTTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGACTTATATTTGTAAATCGGTGGTGGAGGAGAWGAATATATATTGATTTGGTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAAGGTTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTTGCTTGAGATGGCAAGGTGAGAGAGAGTATTGTCAATGCAATAGTGAGAGTAATAGAGGTCATTTGAGACCCCATGTTTTCTCAAAATAGGTTTTGTTGTTDCCCCCCGTACTCYSYCGA
TARGET=406,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02055 Ps_contig_mira-and-tgicl-ass_franssen_18466
SEQUENCE=GTWCGGKGGATGATCCAAACAACAACAACACTCTCATCATCGTCTCATACTTTACCTTTCATTTCAGAGTTACAGCTACATAATCCATTTTAGTTATAGTTAATTAATTTCCAGTTCGATCCATCTTGTTAGTAGTGATCATCATGGCTGAGGAGAATCAGAACAAGTACGAGGAGACTACATCCGCAACCAACTCTGAGACAGAGATCGAAGGACAGGTAGGTTTGTTTTTTAGATTTTTCGCGGGTAGGTAAAGAAAAGGNTGAAGAACACAAGCCTG
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02056 Ps_contig_mira-and-tgicl-ass_franssen_18470
SEQUENCE=CTGAATGAGCTCAGCTTCTCTGTACCATTTGTAGAAATAGCTGGGGTCCCGTTTGGCCCAAGGCCTAGTGGGTCGGAAACCAAAACGTACACCCGGTAGCGATCTGTCGAGCCATCGGGTCGACGAGGTTCYACCGAAACTAACACCAGGGATCCATGATTCTTAGGTGCAGAAGCAGCAGCAGCAACAATAAGTCTTTTAGGACTAACAGCTGCAACACCAACTTTGCTTCCAATGGCAGTAGCTAGCAAGGTTTGGCTCCTCTTTCCACCAGATAAGAAGGAAGATCCCAAACCATTCAACACAGCACCAGATGTTGCAGCTGCCATTTCTATTCTCTTCACACTCTAAAGAAGTTTCACTAAAGATGTATAATGAGATAATAGTAATAGAAGTTTGCTAGATAGTATTTTATGTCCCCGCGTACTCTGCGTTGA
TARGET=174,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02057 Ps_contig_mira-and-tgicl-ass_franssen_18473
SEQUENCE=CGGTTAAAGGTTTTGGTAAAAACCCCCAAACTTAACCAATTAATTTACTTTTCCCTACCATGCAACAAACAAACAAACAACATTATTCATATGACCACAAGGCCAATAGCAAATACAGTTCCATACAGAATGTTGGATAGTTTTAATTGGAGCAACCAGGCCATGCAACATCTCTTGGTGAAAGTGTTTTAAGAACTGGATTCCTCTCAAGAAATTGGTTGGCCTCAGCTCAAATGAGGTGCTCAATAGTGGCATTATTGGGAAATCTTCCTGTGCTGGAACATGATGAATTCCAACTACATGCCACATCACTATGTCCTTGTTCACAATCTCTCTATTCTGTTTAGTCCAAACTGCCAAAGTATCATCACCGCGACTATGATCAACGTAAAGTCCCACCCCGCCCTATTTTTCAGTTCTATTATATGCTGTCCAACCCCAAACTATTTATTAGTYTTAGGTAAAAGCCAACCYAGTTAATTTTGGKAGRTAAWCATCCTNTAGTTAAT
TARGET=64,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02058 Ps_contig_mira-and-tgicl-ass_franssen_18508
SEQUENCE=TTACTGCAGTGGAACTTCTACATGTAAAAGGTACCGGTAGACAAACCATCCAGTGTACCCTAGTCCTACCAACTCCATAATCTTTGGAAGCAAGGGGACTGAGTTGATGGCACCTACAAGAATTGAAGATAACCAAACAGCAACCAAACCACCACCACCATACAGAAGCACTGTGGACTTGTTTTCAAGACCATCCCACTTCTCCTTCAAATCGGTAAACAACTCATTGGTATCGAGAGAAGTAGATGTATCATCAGAAGAAGAAGCTCTAGTCCGATGAAGAGAAAACTTCCGCGACTCTGAAAAATGTTTAGGGAAAGGGAAGAAGAAGGAGTGGTGGTGGAGAGGCGAGGAGGAGGGAGATAAGGCAAAGCAGAGCAGGACGGAGTGKTGGKGKTSRGTWGSYKASGAAGAGGAGGGACTAACACTGTGCTTGTGCTTGCTGCCATTGCCATTAACTCTTGTTTCTCTCTCTCTCTCACTTCCTTTTTTTNGTTATCTGCTTTCTTTGTTTTCCCCGAGTTACTCTGCGTTGA
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02059 Ps_contig_mira-and-tgicl-ass_franssen_18508
SEQUENCE=TTACTGCAGTGGAACTTCTACATGTAAAAGGTACCGGTAGACAAACCATCCAGTGTACCCTAGTCCTACCAACTCCATAATCTTTGGAAGCAAGGGGACTGAGTTGATGGCACCTACAAGAATTGAAGATAACCAAACAGCAACCAAACCACCACCACCATACAGAAGCACTGTGGACTTGTTTTCAAGACCATCCCACTTCTCCTTCAAATCGGTAAACAACTCATTGGTATCGAGAGAAGTAGATGTATCATCAGAAGAAGAAGCTCTAGTCCGATGAAGAGAAAACTTCCGCGACTCTGAAAAATGTTTAGGGAAAGGGAAGAAGAAGGAGTGGTGGTGGAGAGGCGAGGAGGAGGGAGATAAGGCAAAGCAGAGCAGGACGGAGTGKTGGKGKTSRGTWGSYKASGAAGAGGAGGGACTAACACTGTGCTTGTGCTTGCTGCCATTGCCATTAACTCTTGTTTCTCTCTCTCTCTCACTTCCTTTTTTTNGTTATCTGCTTTCTTTGTTTTCCCCGAGTTACTCTGCGTTGA
TARGET=467,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02060 Ps_contig_mira-and-tgicl-ass_franssen_18521
SEQUENCE=GAGARDAATGGCCAAAAGAGAATATCATTATTTTTATAATTTAAAGTTCACAATTACAACCATACATCACACATGGGCTGGGCCAGATTCCAACTTAAAAGTAATGAAAAAACTGCCCCATCACCCAACGCACTCACCACCTAATAATAATAATCATTACAACAACACGTTACACTTACAACAACATCCTTTACATAACAAAAAGGGAATAGTTAAAACAAGATAATGATCGAATTTACAAAATTAAAAAACTAAAACCCCCACCAAAGGTTTGGGTTTTTAGGGTTTAARAG
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02061 Ps_contig_mira-and-tgicl-ass_franssen_18529
SEQUENCE=GTCGGGGKAAAACAAGAAAGCAGATAACAGAAAAAAGGTGTGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGCAGCAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTGCTCTGCTTTGCCTTATCTCCCTCCTCCTCGCCTCTCCACCACCACTCCTTCTTCTTCCCCTTTCCCTAAACTATTTTTCAGAGTCGCGGAAGTTTTCTCTTCATCGGACTAGAGCTTCTTCTGATGATACATCTACTTCTCTCGATACCAATGAGTTGTTTGCCGATTTGAAGGAGAAGTGGGATGGTCTTGAAAACAAGTCCACAGTGCTTCTGTATGGTGGTGGTGGTTTGGTTG
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02062 Ps_contig_mira-and-tgicl-ass_franssen_18529
SEQUENCE=GTCGGGGKAAAACAAGAAAGCAGATAACAGAAAAAAGGTGTGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGCAGCAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTGCTCTGCTTTGCCTTATCTCCCTCCTCCTCGCCTCTCCACCACCACTCCTTCTTCTTCCCCTTTCCCTAAACTATTTTTCAGAGTCGCGGAAGTTTTCTCTTCATCGGACTAGAGCTTCTTCTGATGATACATCTACTTCTCTCGATACCAATGAGTTGTTTGCCGATTTGAAGGAGAAGTGGGATGGTCTTGAAAACAAGTCCACAGTGCTTCTGTATGGTGGTGGTGGTTTGGTTG
TARGET=356,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02063 Ps_contig_mira-and-tgicl-ass_franssen_18551
SEQUENCE=GAAAGTAGGAAAACCAACCCGAAAACCCGTAGATTCTTGGCGTCGGTCCGGCGAAGATCCAGTTTGAAGGTGCTGAAATGAGTGCTGCTTCTGAGGATGGTGGCTGCAAGTGTGGTGATAACTGCACTTGTGACCCTTGCAACTGACAAATGAAGTGTAACATAATAAAAGCTTGAGGCAGAGATATTAGAAACCATTATTGTTTAATTGTGTGTATGAGTACACATGTGTTGTTTGTYYTTTTTAAGAAATTAATTAATTAAATTTAAGTTGTTAGGTACAAAAAAGT
TARGET=250,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02064 Ps_contig_mira-and-tgicl-ass_franssen_18574
SEQUENCE=TATTACACAAAAGGTGATGCTGCTGTTCTTTCCTACTGAAGAGCAACTCAAGAGTGACAAAGCTAAGGGTGATGGTCTTTTCAAGGCTCTTGAGGGTTACTGTTTGGCTCATCCTGATTACAACTAAACTATATAATCATCAACAAGTGTGTGTTGTTATGTATACTATATAATCATCATGTGTGCTTAATTTGGCTGCCAATGTTAATTTTCTTGTTTTTCGTTTTTTCCCTTTTCTTTTCTTGTGGAACTAAAGTGTGAGTATTAGTAACGYCTATTGTTAACTTACCCTTCCTTCCTTACAAATTAAAATTTAAATTAAAAATTAATTTAATTAAAAAAAAA
TARGET=290,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02065 Ps_contig_mira-and-tgicl-ass_franssen_18586
SEQUENCE=CGTGCCTCCCTTCGGGGGAGAGTGACCGGTGATCCCAATCTCACAGAGGATGAACGAAACAAAATAATAGAGTTTCTGGAAGCTTTTCCCAGTGAGGATCTCTGAACTGTCAACGCGCTTGCTTGTTGATATGTAAAGTAGTAGGTGCATGTATATAATGTATTCCATGTAATAAAGATTGAATCAGAACCTTCAAATCTCTGTTAATGTCAATGTAATATTAGCATGTGAAGCTTGTTAGGTTCCATGGCAATCAAATGTCTTTTGCATTCTTCAATTAGCACAACATTTCTAATAAACCATGATATTGTAACGTAAATAAATGTGAGATGAAGAGTTGCTATTCTTCATGACDTATATATATACCAAGTAAAATTTTGAGTCCTTACGTTAACCTAAAAAAACCCT
TARGET=356,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02066 Ps_contig_mira-and-tgicl-ass_franssen_18600
SEQUENCE=CGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAACGAAGTAAATGATGCCAAATATGGCGGTGGACACTATGGTGGTGGACACTATGGAGGTGGACACTATGGTGGTGGTCACTACGGTCATGGTGGTGGTCATCATGGTGGACATGGTGGTGCTTCCGACAATGGTAACTGATGATACCATGATAAATGATGCACATTCAAAGTAATAAGTTTCATGAATGAATAATGTTTGGTGTTAAATAAAAGAAATACCAATT
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02067 Ps_contig_mira-and-tgicl-ass_franssen_18617
SEQUENCE=TGTTTGGTAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTTACTGTGTACACTT
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02068 Ps_contig_mira-and-tgicl-ass_franssen_18627
SEQUENCE=GTCGGGGGTGTCTGCGCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCGGACCTTTGCCGGAGAATGGTCTCGGCAAGCTACCGAAAACTCCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGAATCAGGAGGGTGGAAACGTGAGACTCCGTAGGGTGGGATTCGTTAGGAGGGTNCAGTAGAAAGTGACAGTAGTATCATGATAGTAGAAACCNGCGTACGGG
TARGET=113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2997
PRIMER_LEFT_PENALTY=0.275745
PRIMER_RIGHT_PENALTY=0.023913
PRIMER_LEFT_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_SEQUENCE=CCGTACGCNGGTTTCTACTATC
PRIMER_LEFT=30,22
PRIMER_RIGHT=246,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.3643
PRIMER_LEFT_1_PENALTY=0.275745
PRIMER_RIGHT_1_PENALTY=0.088563
PRIMER_LEFT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_1_SEQUENCE=CCTGATTCCTTGTCGGAGATAG
PRIMER_LEFT_1=30,22
PRIMER_RIGHT_1=152,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=60.089
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=123
PRIMER_PAIR_PENALTY_2=0.6643
PRIMER_LEFT_2_PENALTY=0.640429
PRIMER_RIGHT_2_PENALTY=0.023913
PRIMER_LEFT_2_SEQUENCE=GTGTCTGCGCTTTCCATATTCT
PRIMER_RIGHT_2_SEQUENCE=CCGTACGCNGGTTTCTACTATC
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=60.640
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=3.75
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=0.6994
PRIMER_LEFT_3_PENALTY=0.275745
PRIMER_RIGHT_3_PENALTY=0.423703
PRIMER_LEFT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_3_SEQUENCE=ATTCCTTGTCGGAGATAGATCG
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=148,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.576
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=0.7290
PRIMER_LEFT_4_PENALTY=0.640429
PRIMER_RIGHT_4_PENALTY=0.088563
PRIMER_LEFT_4_SEQUENCE=GTGTCTGCGCTTTCCATATTCT
PRIMER_RIGHT_4_SEQUENCE=CCTGATTCCTTGTCGGAGATAG
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=152,22
PRIMER_LEFT_4_TM=60.640
PRIMER_RIGHT_4_TM=60.089
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_02069 Ps_contig_mira-and-tgicl-ass_franssen_18644
SEQUENCE=CGCAGAGTACGCGGGGACACAGACCACAATCATCATATCCATAGACCCTTCTTTATTACTATTGTTACCAATATCAGCATCATCATCATCATCACATTCRAGTTCAAGAAAGAAAGAAACTAACTATGGCTCAAGCTATGGCATCATCAATGGCTGGTTGTTTACGCGGTTGTTCATCTCAAACTGTGTTAGAAGGAAGTCTTCAATTTAGTTGGACCAAACCAAGGTTGTAGTTTGTTGCACGGAAACACCAACAACGTTAACAAGGTTACAACACGTTCATCAGTGACGGTTAGAGCTCAACAACAGGAGAGTAGTCGAAGGGCTGTGATTGGTCTTGTT
TARGET=78,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02070 Ps_contig_mira-and-tgicl-ass_franssen_18644
SEQUENCE=CGCAGAGTACGCGGGGACACAGACCACAATCATCATATCCATAGACCCTTCTTTATTACTATTGTTACCAATATCAGCATCATCATCATCATCACATTCRAGTTCAAGAAAGAAAGAAACTAACTATGGCTCAAGCTATGGCATCATCAATGGCTGGTTGTTTACGCGGTTGTTCATCTCAAACTGTGTTAGAAGGAAGTCTTCAATTTAGTTGGACCAAACCAAGGTTGTAGTTTGTTGCACGGAAACACCAACAACGTTAACAAGGTTACAACACGTTCATCAGTGACGGTTAGAGCTCAACAACAGGAGAGTAGTCGAAGGGCTGTGATTGGTCTTGTT
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02071 Ps_contig_mira-and-tgicl-ass_franssen_18657
SEQUENCE=TCGGGGACACCAAAACCTTTAACCCATCCTTTCTCTTCTTAGCCGAACACAATCATGGCATCAATGACCATACTACCCTTCTTTCTCTTCTTCTTCTTTATCACTTTCTCATTAGCATCAAACATACCAAATGGTCGTAATAACGAGGAGGTTATGACCATGTACGAGGAGTGGTTGGTAAAACACCAAAAAGTGTATAACGGGTTAGGAGAGAAAGACCAAAGATTTCAAATTTTTAAGGATAATTTAAAATGTTTCTCGGTGTTACTGCATCCGTGACCAATTGGGATGCTGAAGGAACTTGTTGTAGTATTGTTTTGGAAGATAATCCTTTTTGATAAGAATTCGACAATGATTTTAATACAA
TARGET=86,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1245
PRIMER_LEFT_PENALTY=0.014720
PRIMER_RIGHT_PENALTY=0.109754
PRIMER_LEFT_SEQUENCE=CCTTTCTCTTCTTAGCCGAACA
PRIMER_RIGHT_SEQUENCE=CGACCATTTGGTATGTTTGATG
PRIMER_LEFT=27,22
PRIMER_RIGHT=136,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=0.1247
PRIMER_LEFT_1_PENALTY=0.014945
PRIMER_RIGHT_1_PENALTY=0.109754
PRIMER_LEFT_1_SEQUENCE=ACCAAAACCTTTAACCCATCCT
PRIMER_RIGHT_1_SEQUENCE=CGACCATTTGGTATGTTTGATG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=136,22
PRIMER_LEFT_1_TM=59.985
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.1296
PRIMER_LEFT_2_PENALTY=0.019805
PRIMER_RIGHT_2_PENALTY=0.109754
PRIMER_LEFT_2_SEQUENCE=CATCCTTTCTCTTCTTAGCCGA
PRIMER_RIGHT_2_SEQUENCE=CGACCATTTGGTATGTTTGATG
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=136,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=0.1394
PRIMER_LEFT_3_PENALTY=0.014720
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=CCTTTCTCTTCTTAGCCGAACA
PRIMER_RIGHT_3_SEQUENCE=CCACTCCTCGTACATGGTCATA
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=173,22
PRIMER_LEFT_3_TM=60.015
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=0.1396
PRIMER_LEFT_4_PENALTY=0.014945
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=ACCAAAACCTTTAACCCATCCT
PRIMER_RIGHT_4_SEQUENCE=CCACTCCTCGTACATGGTCATA
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=173,22
PRIMER_LEFT_4_TM=59.985
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_02072 Ps_contig_mira-and-tgicl-ass_franssen_18658
SEQUENCE=CCGGTTCCCAGATCTTTGCGGAGGGTGGCCTTGACTATTTGGGCAACCCAAACCTGATTCATGCTCAGAGCATTCTTGCAATCTGGGCTACTCAGGTTGTGCTCATGGGATTTGTTGAAGGCTACAGAGTTGTAGGAGGACCCCTCGGCGAAGGACTAGACCCACTTTACCCTGGTGGTGCTTTTGACCCTCTTGGCCTGTACTGGTGGACGAATAATTTCGTCCCTCTTCCAGAACGTAGCGTGAAGAAGAAGAAGAACGACCCAAAAAGTGGGAAA
TARGET=245,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02073 Ps_contig_mira-and-tgicl-ass_franssen_18669
SEQUENCE=AAACGAAACGGCTCTCTAGCTCCTTACCGGAAAACGGAGATTAGCGGCGGAGTAGAACCAGCGGGGGAAGCCGGAGCTGATCCACTTGGACTCTGGTACCCAGCTGGTTTCACTATCATGATAGTGAAACCAGCTGGTTTCACTATCATGATACTACGTGTCACTTTCACTGCACCCTCTAACGAATCCCCACCGTAGGATCTCACGTTTCCACCCTCTGATTCCTTGTCGGAGATAGAGTCGGGAAAGGAAGGAAGGGTTGTTTTCGGAGCTTGCCGACG
TARGET=247,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02074 Ps_contig_mira-and-tgicl-ass_franssen_18675
SEQUENCE=CCTGCTGAGCTGTGCTGCATATTTCACAAGANCTGTTGGGTGATCAATGATTTCTGGTTGGACCATTCATTGGTGCAGCACTTGCAGCACTTTACCACACAGTTGTTATCAGAGCCACTCCTTTCAAGTCAAGTTGATATGATCCAACGGTTTGTGATAGTTGATGCCAAAACCATTAAACCAAACCAACTTTGTGGGGTTTTATTTTTAATTTGAAATTCGATCATTATCTTGTTTTAACTATTTCCCTTTTTGTATGTAAAGGATGTTGTTGTAAGTGTAACGTGTTGTTGTAATGATTATTATTATTAGGTGGTGAGTGCGTTGGGTGATGGGGCAGTTTTTTCATTACTTTTAAGTTGGAATCTGGCCCAGCCCATGTGTGATGTATGGTTGTAATTGTGAACTTTAAATTATAAAAATAATGATATTCTCTTTTAACRRWAAGG
TARGET=299,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02075 Ps_contig_mira-and-tgicl-ass_franssen_18678
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGCTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTNTAATTGGGTTGCAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTA
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.7981
PRIMER_LEFT_PENALTY=0.363436
PRIMER_RIGHT_PENALTY=2.434710
PRIMER_LEFT_SEQUENCE=GAAGTTGAAAGGATTGGTGCTT
PRIMER_RIGHT_SEQUENCE=GAGATACTGTAATCTGCAACCCAA
PRIMER_LEFT=5,22
PRIMER_RIGHT=198,24
PRIMER_LEFT_TM=59.637
PRIMER_RIGHT_TM=59.565
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=2.8059
PRIMER_LEFT_1_PENALTY=0.371199
PRIMER_RIGHT_1_PENALTY=2.434710
PRIMER_LEFT_1_SEQUENCE=ATACGTTCTTCAATGGCGAAAC
PRIMER_RIGHT_1_SEQUENCE=GAGATACTGTAATCTGCAACCCAA
PRIMER_LEFT_1=84,22
PRIMER_RIGHT_1=198,24
PRIMER_LEFT_1_TM=60.371
PRIMER_RIGHT_1_TM=59.565
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=2.9059
PRIMER_LEFT_2_PENALTY=0.471231
PRIMER_RIGHT_2_PENALTY=2.434710
PRIMER_LEFT_2_SEQUENCE=CATCAAGCAGGATCGGATAGTT
PRIMER_RIGHT_2_SEQUENCE=GAGATACTGTAATCTGCAACCCAA
PRIMER_LEFT_2=29,22
PRIMER_RIGHT_2=198,24
PRIMER_LEFT_2_TM=60.471
PRIMER_RIGHT_2_TM=59.565
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=3.0639
PRIMER_LEFT_3_PENALTY=0.629225
PRIMER_RIGHT_3_PENALTY=2.434710
PRIMER_LEFT_3_SEQUENCE=GTTGGAAGTTGAAAGGATTGGT
PRIMER_RIGHT_3_SEQUENCE=GAGATACTGTAATCTGCAACCCAA
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=198,24
PRIMER_LEFT_3_TM=59.371
PRIMER_RIGHT_3_TM=59.565
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=3.0869
PRIMER_LEFT_4_PENALTY=0.363436
PRIMER_RIGHT_4_PENALTY=2.723447
PRIMER_LEFT_4_SEQUENCE=GAAGTTGAAAGGATTGGTGCTT
PRIMER_RIGHT_4_SEQUENCE=GAGATACTGTAATCTGCAACCCA
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=198,23
PRIMER_LEFT_4_TM=59.637
PRIMER_RIGHT_4_TM=58.277
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_02076 Ps_contig_mira-and-tgicl-ass_franssen_18701
SEQUENCE=GATSATCCAAACAACAACAACACTATCATCATCGTCTCATACTTTACCTTTCATTTCAGAGTTACAGCTACATAATCCTATTTTAGTTACRGTTAATTAATTTCCAGTTCGATCCATTCTTGTTAGTAGTGATCATCATGGCTGGAGGAGAATCARGAACAAGGTACGAGGACGACCTACCCTCCGACCAAACCCAACTYCKAGRMAGACATACGAAAGGAC
TARGET=10,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02077 Ps_contig_mira-and-tgicl-ass_franssen_18706
SEQUENCE=TCGGGGKAACAACAACAACAAGAAGAACAAGGGTTTTTCACTTCCTCGTTTAAATCGAAAACATTAACCAAAACATGGGCAGAGTTACAGCTACATAATCCATTTTAGTTACAGTTAATTAATTTCCAGTCCGATCCATCTTGTTAGTAGTGATCATCATGGCTGAGGAGAATCAGAACAAGTACGAGGAGACTACCTCCGCAACCAACTCTGAGACAGAGATCAAGGACAGAGGTTGTTTTTGTATTTTCTC
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02078 Ps_contig_mira-and-tgicl-ass_franssen_18716
SEQUENCE=GGTAGAAGTGTACACAAGTAAAAAAAGACTCACAACTACTATTAAGAGATACTGTAATCTACAACCAATTATACTTTTCTTTCTTTCAATTAGTCTGTCTCAACACCTAATCCATACAACACACCAGAGTATTTCTCTGTTTCGCCATTGAAGAACGTATCCCTCAATTTTCTAAATGCACAAGCAGTAGAAGTAAACTATCCGATTCCTGTCTTGATGGACAAACACCATCCTTNACTCANACTCNGCGTNACTACCNCT
TARGET=76,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02079 Ps_contig_mira-and-tgicl-ass_franssen_18722
SEQUENCE=AACGCAGAGTACGCGGGACAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCATACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTTGTTTTTGWTGTTATTACGCCACAGCTGTAAGGCACTGCCCTCARTATGCATGWRTCTGYARTTGTTTGCATRARTTKYRRTTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTATAGTAAAA
TARGET=149,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02080 Ps_contig_mira-and-tgicl-ass_franssen_18722
SEQUENCE=AACGCAGAGTACGCGGGACAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCATACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTTGTTTTTGWTGTTATTACGCCACAGCTGTAAGGCACTGCCCTCARTATGCATGWRTCTGYARTTGTTTGCATRARTTKYRRTTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTATAGTAAAA
TARGET=170,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02081 Ps_contig_mira-and-tgicl-ass_franssen_18722
SEQUENCE=AACGCAGAGTACGCGGGACAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCATACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTTGTTTTTGWTGTTATTACGCCACAGCTGTAAGGCACTGCCCTCARTATGCATGWRTCTGYARTTGTTTGCATRARTTKYRRTTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTATAGTAAAA
TARGET=367,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02082 Ps_contig_mira-and-tgicl-ass_franssen_18722
SEQUENCE=AACGCAGAGTACGCGGGACAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCATACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTTGTTTTTGWTGTTATTACGCCACAGCTGTAAGGCACTGCCCTCARTATGCATGWRTCTGYARTTGTTTGCATRARTTKYRRTTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTATAGTAAAA
TARGET=418,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02083 Ps_contig_mira-and-tgicl-ass_franssen_18722
SEQUENCE=AACGCAGAGTACGCGGGACAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCATACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTTGTTTTTGWTGTTATTACGCCACAGCTGTAAGGCACTGCCCTCARTATGCATGWRTCTGYARTTGTTTGCATRARTTKYRRTTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTATAGTAAAA
TARGET=464,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02084 Ps_contig_mira-and-tgicl-ass_franssen_18722
SEQUENCE=AACGCAGAGTACGCGGGACAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCATACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTTGTTTTTGWTGTTATTACGCCACAGCTGTAAGGCACTGCCCTCARTATGCATGWRTCTGYARTTGTTTGCATRARTTKYRRTTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTATAGTAAAA
TARGET=524,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02085 Ps_contig_mira-and-tgicl-ass_franssen_18722
SEQUENCE=AACGCAGAGTACGCGGGACAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCATACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTTGTTTTTGWTGTTATTACGCCACAGCTGTAAGGCACTGCCCTCARTATGCATGWRTCTGYARTTGTTTGCATRARTTKYRRTTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTATAGTAAAA
TARGET=740,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02086 Ps_contig_mira-and-tgicl-ass_franssen_18725
SEQUENCE=TGGACATGGAGGCTACGGTCATGGAGGCTACGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCGTCAGGGAAGTTATTGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGCGGTGAACACTATGGTGGTGGACACTATGGAGGTGGACACTATGGTGGTGGTCACTACGGTCATGGTGGTGGTCATCATGGTGGACATGGTGGTGCTTCCGACAATGGTAACTGATGATACCATGATAAAATGA
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3084
PRIMER_LEFT_PENALTY=2.042732
PRIMER_RIGHT_PENALTY=0.265715
PRIMER_LEFT_SEQUENCE=GTCATGGAGGCTACGGTCAT
PRIMER_RIGHT_SEQUENCE=CTCCATAGTGTCCACCACCATA
PRIMER_LEFT=17,20
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=59.957
PRIMER_RIGHT_TM=59.734
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=2.3084
PRIMER_LEFT_1_PENALTY=2.042732
PRIMER_RIGHT_1_PENALTY=0.265715
PRIMER_LEFT_1_SEQUENCE=GACATGGAGGCTACGGTCAT
PRIMER_RIGHT_1_SEQUENCE=CTCCATAGTGTCCACCACCATA
PRIMER_LEFT_1=2,20
PRIMER_RIGHT_1=244,22
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=59.734
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=2.3084
PRIMER_LEFT_2_PENALTY=2.042732
PRIMER_RIGHT_2_PENALTY=0.265715
PRIMER_LEFT_2_SEQUENCE=GTCATGGAGGCTACGGTCAT
PRIMER_RIGHT_2_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT_2=17,20
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=59.957
PRIMER_RIGHT_2_TM=59.734
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=243
PRIMER_PAIR_PENALTY_3=2.3084
PRIMER_LEFT_3_PENALTY=2.042732
PRIMER_RIGHT_3_PENALTY=0.265715
PRIMER_LEFT_3_SEQUENCE=GACATGGAGGCTACGGTCAT
PRIMER_RIGHT_3_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT_3=2,20
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=59.957
PRIMER_RIGHT_3_TM=59.734
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=2.3387
PRIMER_LEFT_4_PENALTY=2.042732
PRIMER_RIGHT_4_PENALTY=0.295944
PRIMER_LEFT_4_SEQUENCE=GTCATGGAGGCTACGGTCAT
PRIMER_RIGHT_4_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_4=17,20
PRIMER_RIGHT_4=253,22
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=60.296
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_02087 Ps_contig_mira-and-tgicl-ass_franssen_18725
SEQUENCE=TGGACATGGAGGCTACGGTCATGGAGGCTACGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCGTCAGGGAAGTTATTGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGCGGTGAACACTATGGTGGTGGACACTATGGAGGTGGACACTATGGTGGTGGTCACTACGGTCATGGTGGTGGTCATCATGGTGGACATGGTGGTGCTTCCGACAATGGTAACTGATGATACCATGATAAAATGA
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3084
PRIMER_LEFT_PENALTY=2.042732
PRIMER_RIGHT_PENALTY=0.265715
PRIMER_LEFT_SEQUENCE=GTCATGGAGGCTACGGTCAT
PRIMER_RIGHT_SEQUENCE=CTCCATAGTGTCCACCACCATA
PRIMER_LEFT=17,20
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=59.957
PRIMER_RIGHT_TM=59.734
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=2.3084
PRIMER_LEFT_1_PENALTY=2.042732
PRIMER_RIGHT_1_PENALTY=0.265715
PRIMER_LEFT_1_SEQUENCE=GACATGGAGGCTACGGTCAT
PRIMER_RIGHT_1_SEQUENCE=CTCCATAGTGTCCACCACCATA
PRIMER_LEFT_1=2,20
PRIMER_RIGHT_1=244,22
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=59.734
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=2.3084
PRIMER_LEFT_2_PENALTY=2.042732
PRIMER_RIGHT_2_PENALTY=0.265715
PRIMER_LEFT_2_SEQUENCE=GTCATGGAGGCTACGGTCAT
PRIMER_RIGHT_2_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT_2=17,20
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=59.957
PRIMER_RIGHT_2_TM=59.734
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=243
PRIMER_PAIR_PENALTY_3=2.3084
PRIMER_LEFT_3_PENALTY=2.042732
PRIMER_RIGHT_3_PENALTY=0.265715
PRIMER_LEFT_3_SEQUENCE=GACATGGAGGCTACGGTCAT
PRIMER_RIGHT_3_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT_3=2,20
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=59.957
PRIMER_RIGHT_3_TM=59.734
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=2.3387
PRIMER_LEFT_4_PENALTY=2.042732
PRIMER_RIGHT_4_PENALTY=0.295944
PRIMER_LEFT_4_SEQUENCE=GTCATGGAGGCTACGGTCAT
PRIMER_RIGHT_4_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_4=17,20
PRIMER_RIGHT_4=253,22
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=60.296
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_02088 Ps_contig_mira-and-tgicl-ass_franssen_18741
SEQUENCE=CTACTTCTCTCGACTAACCAATGAGTTTGGTTTTACCGTATTTTGGAAAGGGAAGAAGGTGGGATGGTTCTTAGAAAACAAGTCCACAGTGCTTCTGTATGGTGGTGGTGGTTTGGTTGCTGTTTGGTTATCTTCAATTCTTGTAGGTGCCATCAACTCAGTCCCCTTGCTTCCAAAGATTATGGAGTTGGTAGGACTAGGGTACACTGGATGGTTTGTCTACCGGTACCTTCTGTTTAAGTCTAGTAGAAAGGAACTAGCTACGGATATTGAGGCACTGAAAAAAGAAAATTGCCGGAACCGAATAGAGCTGTATTAGTAGCAAGTCATGTTAAAGAAAATAAACTTAACCTTAGACTCATCATTTGTTTGTTTTGCTGTATGTTCTTGTTATTATGAGAGGCATTTGTATCTCTTGATATAGAATAGAATTGCTCAATCTCTATCAGTATCAACCTTGTATTAATCCATTTCATTGTTGAAAATAATGCAGCAAATCTTATATATATGTTTATGATTCTGAATGAAAATCATTCATAAAAATTTTT
TARGET=100,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1459
PRIMER_LEFT_PENALTY=0.142283
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=ACCAATGAGTTTGGTTTTACCG
PRIMER_RIGHT_SEQUENCE=TTGATGGCACCTACAAGAATTG
PRIMER_LEFT=16,22
PRIMER_RIGHT=155,22
PRIMER_LEFT_TM=60.142
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=140
PRIMER_PAIR_PENALTY_1=0.1459
PRIMER_LEFT_1_PENALTY=0.142283
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=CCAATGAGTTTGGTTTTACCGT
PRIMER_RIGHT_1_SEQUENCE=TTGATGGCACCTACAAGAATTG
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=155,22
PRIMER_LEFT_1_TM=60.142
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.3000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=139
PRIMER_PAIR_PENALTY_2=0.3210
PRIMER_LEFT_2_PENALTY=0.142283
PRIMER_RIGHT_2_PENALTY=0.178718
PRIMER_LEFT_2_SEQUENCE=ACCAATGAGTTTGGTTTTACCG
PRIMER_RIGHT_2_SEQUENCE=GACTGAGTTGATGGCACCTACA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=162,22
PRIMER_LEFT_2_TM=60.142
PRIMER_RIGHT_2_TM=60.179
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=0.3210
PRIMER_LEFT_3_PENALTY=0.142283
PRIMER_RIGHT_3_PENALTY=0.178718
PRIMER_LEFT_3_SEQUENCE=CCAATGAGTTTGGTTTTACCGT
PRIMER_RIGHT_3_SEQUENCE=GACTGAGTTGATGGCACCTACA
PRIMER_LEFT_3=17,22
PRIMER_RIGHT_3=162,22
PRIMER_LEFT_3_TM=60.142
PRIMER_RIGHT_3_TM=60.179
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.3456
PRIMER_LEFT_4_PENALTY=0.142283
PRIMER_RIGHT_4_PENALTY=0.203324
PRIMER_LEFT_4_SEQUENCE=ACCAATGAGTTTGGTTTTACCG
PRIMER_RIGHT_4_SEQUENCE=CTGAGTTGATGGCACCTACAAG
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=160,22
PRIMER_LEFT_4_TM=60.142
PRIMER_RIGHT_4_TM=59.797
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_02089 Ps_contig_mira-and-tgicl-ass_franssen_18763
SEQUENCE=TTCGGGGTTGGTTGGTTGGTTTACCTRKWRKTKSAGGTGGACACGGTGGACATGGTGGTGCTTCCAACAACGGTAACTGATAATATCAACATTCTCAGTAATAAATATCATGAATTAATAATGTTGGATGTTRAGAAAAAAAAAGGAGGTTATAGATAGACATGAATTCTTTTATGGTTATGTATTATCTATCTTGCAACTTCTTTAGTATTAGTTTATTTCTCAACTCTAATAAATCAACTATGTATATGNGATGGATGATGTTTA
TARGET=6,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02090 Ps_contig_mira-and-tgicl-ass_franssen_18778
SEQUENCE=CTAACAAACAACTGCAGGGAAACTCAGAAATTTTGGTAAAGGCCAAGATCAACACTGGAGCCAGGATTGGAAATTAAAATACATTACTATCAGCTTTGTATGGGGCTTTCCAAACATATACATATAATATAATATAATAAGTGTATGTGTATGTTTTTTGGGTAATAATAGTAATAAAATAAAGTGTTTTTATCTCTATCTTATGAAGTGTGTTTTTGTGTAGTAAAAATAAAATAATAATAAAATTGGTAGTAGTTWAAT
TARGET=122,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02091 Ps_contig_mira-and-tgicl-ass_franssen_18794
SEQUENCE=TTTAAGGTTCGTAACCGGAACGAATTTAATTTTAACTTTAACTAACCAACAATTTTAATTTGTAAGTTTCTAAAGAAAGAAGCAACTACANATACATAGATAGATGCAGTGCTAGCTACTACAGCATGCATTTTATTTATTTATTTATATTATGCGTCTATCTCTTTGTGTTGGAGAATCAATCCATCCCTCCAAGTTTAACAGTTTTGAGTGCAGAAACATTTCCAGTTATGGCACGTGCCACTGATTAAGTGCGCTTTGTTCTTGCAGTGATCATCACAGCTAGCATTCGTGAAGCATACCTCCCTGTATGTATCAGCCAAATGCTCAACTAAGTTTTTCGCCTCTTCGTCACCACTATTTCTTGTGACAAACAAAGG
TARGET=132,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4089
PRIMER_LEFT_PENALTY=0.232153
PRIMER_RIGHT_PENALTY=0.176730
PRIMER_LEFT_SEQUENCE=GTTCGTAACCGGAACGAATTTA
PRIMER_RIGHT_SEQUENCE=TTAAACTTGGAGGGATGGATTG
PRIMER_LEFT=6,22
PRIMER_RIGHT=200,22
PRIMER_LEFT_TM=60.232
PRIMER_RIGHT_TM=60.177
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.4355
PRIMER_LEFT_1_PENALTY=0.232153
PRIMER_RIGHT_1_PENALTY=0.203367
PRIMER_LEFT_1_SEQUENCE=GTTCGTAACCGGAACGAATTTA
PRIMER_RIGHT_1_SEQUENCE=GATGGATTGATTCTCCAACACA
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=187,22
PRIMER_LEFT_1_TM=60.232
PRIMER_RIGHT_1_TM=59.797
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=0.4520
PRIMER_LEFT_2_PENALTY=0.232153
PRIMER_RIGHT_2_PENALTY=0.219884
PRIMER_LEFT_2_SEQUENCE=GTTCGTAACCGGAACGAATTTA
PRIMER_RIGHT_2_SEQUENCE=ACTGGAAATGTTTCTGCACTCA
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=228,22
PRIMER_LEFT_2_TM=60.232
PRIMER_RIGHT_2_TM=59.780
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.5158
PRIMER_LEFT_3_PENALTY=0.232153
PRIMER_RIGHT_3_PENALTY=0.283676
PRIMER_LEFT_3_SEQUENCE=GTTCGTAACCGGAACGAATTTA
PRIMER_RIGHT_3_SEQUENCE=CTGGAAATGTTTCTGCACTCAA
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=227,22
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=60.284
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.5283
PRIMER_LEFT_4_PENALTY=0.232153
PRIMER_RIGHT_4_PENALTY=0.296177
PRIMER_LEFT_4_SEQUENCE=GTTCGTAACCGGAACGAATTTA
PRIMER_RIGHT_4_SEQUENCE=TGTTAAACTTGGAGGGATGGAT
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=202,22
PRIMER_LEFT_4_TM=60.232
PRIMER_RIGHT_4_TM=59.704
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=197
=
PRIMER_SEQUENCE_ID=SSR_02092 Ps_contig_mira-and-tgicl-ass_franssen_18802
SEQUENCE=CTCCTTAATTTTGTCAAGGAAACCCTTCTTTTCCTCAGTTTACCGGTTTTGTTTTCCTTTTCTAACTTAACCTTCTAACTTTTCTCAACTGGCACCGAAGTGTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTTCTCACCATCTTCTCCTTCCTCTTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGGTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCACCTTGTGAACCAAACTCGTGGGCAATCCGCCTCATTCTTGAGCAGGTCTTATTGTTTCCTTCTACTTTCCCTTTTTTA
TARGET=123,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1055
PRIMER_LEFT_PENALTY=0.093130
PRIMER_RIGHT_PENALTY=0.012411
PRIMER_LEFT_SEQUENCE=TCCTCAGTTTACCGGTTTTGTT
PRIMER_RIGHT_SEQUENCE=CCTCAAGTGAAGAGGAAGGAGA
PRIMER_LEFT=31,22
PRIMER_RIGHT=171,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.1055
PRIMER_LEFT_1_PENALTY=0.093130
PRIMER_RIGHT_1_PENALTY=0.012411
PRIMER_LEFT_1_SEQUENCE=TCCTCAGTTTACCGGTTTTGTT
PRIMER_RIGHT_1_SEQUENCE=TCCTCAAGTGAAGAGGAAGGAG
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=172,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=59.988
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=142
PRIMER_PAIR_PENALTY_2=0.1055
PRIMER_LEFT_2_PENALTY=0.093130
PRIMER_RIGHT_2_PENALTY=0.012411
PRIMER_LEFT_2_SEQUENCE=TTCCTCAGTTTACCGGTTTTGT
PRIMER_RIGHT_2_SEQUENCE=CCTCAAGTGAAGAGGAAGGAGA
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=171,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=59.988
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.1055
PRIMER_LEFT_3_PENALTY=0.093130
PRIMER_RIGHT_3_PENALTY=0.012411
PRIMER_LEFT_3_SEQUENCE=TTCCTCAGTTTACCGGTTTTGT
PRIMER_RIGHT_3_SEQUENCE=TCCTCAAGTGAAGAGGAAGGAG
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=172,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=59.988
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=0.1055
PRIMER_LEFT_4_PENALTY=0.093130
PRIMER_RIGHT_4_PENALTY=0.012411
PRIMER_LEFT_4_SEQUENCE=TCCTCAGTTTACCGGTTTTGTT
PRIMER_RIGHT_4_SEQUENCE=CTCCTCAAGTGAAGAGGAAGGA
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=173,22
PRIMER_LEFT_4_TM=59.907
PRIMER_RIGHT_4_TM=59.988
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_02093 Ps_contig_mira-and-tgicl-ass_franssen_18816
SEQUENCE=TAGTGTGATGCCAATGATTGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTAAACCGGAGGAGGAGAATGGTAAACTGGGGTAGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGTGGAGGAGAGTGGTACACTGGGTGTGGGTGAGGGTAGGTGTACGGTGGTGGTGGAGATCTAACAAATGTAGGAGCAGCAGGGAAAGATGATACATATGCATTAGTCAAACGCCCAGCTTTGGCAGGTGGCATATGGCCTCCATTGCTTGTTGCTTGCTTCTTGTTTCTTTTATGGCAGGGACAGTA
TARGET=143,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2358
PRIMER_LEFT_PENALTY=0.032476
PRIMER_RIGHT_PENALTY=0.203343
PRIMER_LEFT_SEQUENCE=GAGTAAACCGGAGGAGGAGAAT
PRIMER_RIGHT_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_LEFT=80,22
PRIMER_RIGHT=220,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.203
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.2358
PRIMER_LEFT_1_PENALTY=0.032476
PRIMER_RIGHT_1_PENALTY=0.203343
PRIMER_LEFT_1_SEQUENCE=GAGTAAACCGGAGGAGGAGAAT
PRIMER_RIGHT_1_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=223,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=60.203
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=0.2685
PRIMER_LEFT_2_PENALTY=0.032476
PRIMER_RIGHT_2_PENALTY=0.235991
PRIMER_LEFT_2_SEQUENCE=GAGTAAACCGGAGGAGGAGAAT
PRIMER_RIGHT_2_SEQUENCE=GTATCATCTTTCCCTGCTGCTC
PRIMER_LEFT_2=80,22
PRIMER_RIGHT_2=320,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=60.236
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=0.3303
PRIMER_LEFT_3_PENALTY=0.032476
PRIMER_RIGHT_3_PENALTY=0.297793
PRIMER_LEFT_3_SEQUENCE=GAGTAAACCGGAGGAGGAGAAT
PRIMER_RIGHT_3_SEQUENCE=ACACCACACAAGAAGCCTTACA
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=221,22
PRIMER_LEFT_3_TM=59.968
PRIMER_RIGHT_3_TM=59.702
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=142
PRIMER_PAIR_PENALTY_4=0.3394
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.203343
PRIMER_LEFT_4_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_RIGHT_4_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=223,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_02094 Ps_contig_mira-and-tgicl-ass_franssen_18816
SEQUENCE=TAGTGTGATGCCAATGATTGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTAAACCGGAGGAGGAGAATGGTAAACTGGGGTAGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGTGGAGGAGAGTGGTACACTGGGTGTGGGTGAGGGTAGGTGTACGGTGGTGGTGGAGATCTAACAAATGTAGGAGCAGCAGGGAAAGATGATACATATGCATTAGTCAAACGCCCAGCTTTGGCAGGTGGCATATGGCCTCCATTGCTTGTTGCTTGCTTCTTGTTTCTTTTATGGCAGGGACAGTA
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2358
PRIMER_LEFT_PENALTY=0.032476
PRIMER_RIGHT_PENALTY=0.203343
PRIMER_LEFT_SEQUENCE=GAGTAAACCGGAGGAGGAGAAT
PRIMER_RIGHT_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_LEFT=80,22
PRIMER_RIGHT=220,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.203
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.2358
PRIMER_LEFT_1_PENALTY=0.032476
PRIMER_RIGHT_1_PENALTY=0.203343
PRIMER_LEFT_1_SEQUENCE=GAGTAAACCGGAGGAGGAGAAT
PRIMER_RIGHT_1_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=223,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=60.203
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=0.2685
PRIMER_LEFT_2_PENALTY=0.032476
PRIMER_RIGHT_2_PENALTY=0.235991
PRIMER_LEFT_2_SEQUENCE=GAGTAAACCGGAGGAGGAGAAT
PRIMER_RIGHT_2_SEQUENCE=GTATCATCTTTCCCTGCTGCTC
PRIMER_LEFT_2=80,22
PRIMER_RIGHT_2=320,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=60.236
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=0.3303
PRIMER_LEFT_3_PENALTY=0.032476
PRIMER_RIGHT_3_PENALTY=0.297793
PRIMER_LEFT_3_SEQUENCE=GAGTAAACCGGAGGAGGAGAAT
PRIMER_RIGHT_3_SEQUENCE=ACACCACACAAGAAGCCTTACA
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=221,22
PRIMER_LEFT_3_TM=59.968
PRIMER_RIGHT_3_TM=59.702
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=142
PRIMER_PAIR_PENALTY_4=0.3394
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.203343
PRIMER_LEFT_4_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_RIGHT_4_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=223,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_02095 Ps_contig_mira-and-tgicl-ass_franssen_18858
SEQUENCE=CGTTCCGCCGCCACAGTTCGCCGTCTCAAGATGTACAAAGTCAGACCCACACAAGGCAGCAGTTATTGGAGACACCATTGGAGACCCATTGAAGGATACATCTGGACCTTCACTTAACATCCTTATCAAGCTGATGGCAGTTGAGTCACTGGTGTTTGCTCCTTTCTTTGCAACCCACGGTGGTCTCCTCTTCAAGTTTTGGTAATAGAAGAGCATCGTCCAATACCCACCCACCAACCAACCAACCATGGCGTCTCTCCCTTTTTTGTTAGTTTGATGGCAACTTTGTTTTTGTTTTTTATTATTATCTTATTATTACTCCCCCTATCCCCTNTTGTTAGTATTTGAGATACTTTAATTAGGGCCCGTAAATTTCCTTTTTCCCGGCGTTGGTTCTATTTTTCTTAGTAAAAGTCCTAAGTTGATACGATGGC
TARGET=233,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1254
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=0.050923
PRIMER_LEFT_SEQUENCE=ACCCATTGAAGGATACATCTGG
PRIMER_RIGHT_SEQUENCE=AANAGGGGATAGGGGGAGTAAT
PRIMER_LEFT=83,22
PRIMER_RIGHT=335,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.949
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.2351
PRIMER_LEFT_1_PENALTY=0.184188
PRIMER_RIGHT_1_PENALTY=0.050923
PRIMER_LEFT_1_SEQUENCE=GTTGAGTCACTGGTGTTTGCTC
PRIMER_RIGHT_1_SEQUENCE=AANAGGGGATAGGGGGAGTAAT
PRIMER_LEFT_1=139,22
PRIMER_RIGHT_1=335,22
PRIMER_LEFT_1_TM=59.816
PRIMER_RIGHT_1_TM=59.949
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=0.2379
PRIMER_LEFT_2_PENALTY=0.186949
PRIMER_RIGHT_2_PENALTY=0.050923
PRIMER_LEFT_2_SEQUENCE=TTGGAGACCCATTGAAGGATAC
PRIMER_RIGHT_2_SEQUENCE=AANAGGGGATAGGGGGAGTAAT
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=335,22
PRIMER_LEFT_2_TM=60.187
PRIMER_RIGHT_2_TM=59.949
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.2603
PRIMER_LEFT_3_PENALTY=0.209343
PRIMER_RIGHT_3_PENALTY=0.050923
PRIMER_LEFT_3_SEQUENCE=AGTCACTGGTGTTTGCTCCTTT
PRIMER_RIGHT_3_SEQUENCE=AANAGGGGATAGGGGGAGTAAT
PRIMER_LEFT_3=143,22
PRIMER_RIGHT_3=335,22
PRIMER_LEFT_3_TM=60.209
PRIMER_RIGHT_3_TM=59.949
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.3850
PRIMER_LEFT_4_PENALTY=0.334070
PRIMER_RIGHT_4_PENALTY=0.050923
PRIMER_LEFT_4_SEQUENCE=ATTGGAGACCCATTGAAGGATA
PRIMER_RIGHT_4_SEQUENCE=AANAGGGGATAGGGGGAGTAAT
PRIMER_LEFT_4=76,22
PRIMER_RIGHT_4=335,22
PRIMER_LEFT_4_TM=59.666
PRIMER_RIGHT_4_TM=59.949
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=260
=
PRIMER_SEQUENCE_ID=SSR_02096 Ps_contig_mira-and-tgicl-ass_franssen_18863
SEQUENCE=TCGGGGGGCGGTGCAGTGCAGCCTTTTTCTCTGCCCTCCCAAAAATTGAAGAAAAAAACCAAAAAAATTTCAGTTTCTCCGCCGTTACGATCACCGGAGAGTTTATCCCTACTCCATTAACACTCTCAAAGAAACAAATCCAAAAAATTGAACTGCTAAGAAGTGGTTAGGTTTAACTTTATTATTATTACGTTGATTTGTGWAGTTTGATGGCGCAGATTCAGGTTCAGCAGGCCGTTCCGGCACCTTCGAACGGCGTCATTGCCG
TARGET=179,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02097 Ps_contig_mira-and-tgicl-ass_franssen_18889
SEQUENCE=CGTATTTAGTGGTGGTAAGGTGGAGGAGGTGATTGTGTAATAGGCTGGTGGAGTGGGGAGTAAACTGGAGGAGGAGGAGGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGCGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTTAGGRSTKKKKGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAAGTGGTAGACAGGGTGGWGGRTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATTATTTCAGATGGGAAACTAGAAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCAAGTTACGGGACCTCTATTTTGTTTTTCTTATGTAGTTGTTTATTTTTGGTTACCCG
TARGET=67,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02098 Ps_contig_mira-and-tgicl-ass_franssen_18889
SEQUENCE=CGTATTTAGTGGTGGTAAGGTGGAGGAGGTGATTGTGTAATAGGCTGGTGGAGTGGGGAGTAAACTGGAGGAGGAGGAGGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGCGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTTAGGRSTKKKKGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAAGTGGTAGACAGGGTGGWGGRTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATTATTTCAGATGGGAAACTAGAAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCAAGTTACGGGACCTCTATTTTGTTTTTCTTATGTAGTTGTTTATTTTTGGTTACCCG
TARGET=124,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02099 Ps_contig_mira-and-tgicl-ass_franssen_18889
SEQUENCE=CGTATTTAGTGGTGGTAAGGTGGAGGAGGTGATTGTGTAATAGGCTGGTGGAGTGGGGAGTAAACTGGAGGAGGAGGAGGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGCGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTTAGGRSTKKKKGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAAGTGGTAGACAGGGTGGWGGRTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATTATTTCAGATGGGAAACTAGAAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCAAGTTACGGGACCTCTATTTTGTTTTTCTTATGTAGTTGTTTATTTTTGGTTACCCG
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02100 Ps_contig_mira-and-tgicl-ass_franssen_18889
SEQUENCE=CGTATTTAGTGGTGGTAAGGTGGAGGAGGTGATTGTGTAATAGGCTGGTGGAGTGGGGAGTAAACTGGAGGAGGAGGAGGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGCGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTTAGGRSTKKKKGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAAGTGGTAGACAGGGTGGWGGRTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATTATTTCAGATGGGAAACTAGAAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCAAGTTACGGGACCTCTATTTTGTTTTTCTTATGTAGTTGTTTATTTTTGGTTACCCG
TARGET=228,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02101 Ps_contig_mira-and-tgicl-ass_franssen_18889
SEQUENCE=CGTATTTAGTGGTGGTAAGGTGGAGGAGGTGATTGTGTAATAGGCTGGTGGAGTGGGGAGTAAACTGGAGGAGGAGGAGGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGCGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTTAGGRSTKKKKGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAAGTGGTAGACAGGGTGGWGGRTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATTATTTCAGATGGGAAACTAGAAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCAAGTTACGGGACCTCTATTTTGTTTTTCTTATGTAGTTGTTTATTTTTGGTTACCCG
TARGET=269,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02102 Ps_contig_mira-and-tgicl-ass_franssen_18889
SEQUENCE=CGTATTTAGTGGTGGTAAGGTGGAGGAGGTGATTGTGTAATAGGCTGGTGGAGTGGGGAGTAAACTGGAGGAGGAGGAGGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGCGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTTAGGRSTKKKKGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAAGTGGTAGACAGGGTGGWGGRTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATTATTTCAGATGGGAAACTAGAAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCAAGTTACGGGACCTCTATTTTGTTTTTCTTATGTAGTTGTTTATTTTTGGTTACCCG
TARGET=435,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02103 Ps_contig_mira-and-tgicl-ass_franssen_18891
SEQUENCE=GAAGAAGAAGAAAGATAATACTAATTTGMGAATGAACAAAAGGACCATATCATTCATTAACTCTTCTCCATCCTATTTCCATTTTACAGTACCGAAAACCAAAAAAACCGAAAAAAAAACACAGTAAATTACAAGCACAACAATGGTACAAGAGAAACAGTCTTTTCCTAATGCCATAATACTCAAACTTAGTAGGATTCTGGTGTGTGTGCAATAGAAACTGAGTGCCTATTTAGTAAACTTACGGAACGGTAAACGGTTTCGGTACCGAAAACCGAACGANAACGAACGAAG
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02104 Ps_contig_mira-and-tgicl-ass_franssen_18905
SEQUENCE=CGGGGGTGGTAAAGAGGTTGTTGTTGTGGGGATAAGATTGCAGTGAGTGAGTGAGTTTGTGAAACTATGGCTTCTGCTTCAGCATCTATTTTCAAATCATCATCACTTGTTCTTGATAAGTCTGAATGGGTGAAGGAAAACTCTCCTCCGTCAACCTTCTTCTATGTCTGTTGCCAGATCGCCAACCCTGWCTAATTCATACTAACGTTTTACCACTCCGAGTCGTTCGGTTTMTTA
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02105 Ps_contig_mira-and-tgicl-ass_franssen_18909
SEQUENCE=AGCACCTAAAGAAACTGAGGAATCGGTTGAAGAAGTGAAGGAAGAGGCGGAAGCAGTAGTGGAGGAGCCAATAGCAACTACTGAAAATGAGTCAGCACCACCACCACCAGCAACTGCAGAGGATGTGATTGAGAACAAAACAGTTGAATCAGTGGAAGCTCCAGCAGCAGAGGAAGTTCCAGTTGAGAATCCTGAAGCTTAAACCAATGGAATGGGTGTGACTGGAACTAGGTAAAAAACGGTAAGTTTAAATTAATGGTTCTAGATGGTTGGGATCGGGTCTACCTATTTTGCATGTCATCGTAAAGGTTAGAAAAGTTCTTTTTACTTTAATTTAAATTTTATTTTTCCTTTTTAACGTTTTTCCCTTTTACCTTAATTTAATTTTAAAAGTTTTATTTATTTGTTTAATTTAGTTGGTTAGTTGGGTTCGTTTTTGGGAATTAGTTGGTTATC
TARGET=96,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0221
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.019805
PRIMER_LEFT_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_RIGHT_SEQUENCE=ACACCCATTCCATTGGTTTAAG
PRIMER_LEFT=26,22
PRIMER_RIGHT=218,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=193
PRIMER_PAIR_PENALTY_1=0.0550
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.052729
PRIMER_LEFT_1_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_RIGHT_1_SEQUENCE=CTCAACTGGAACTTCCTCTGCT
PRIMER_LEFT_1=26,22
PRIMER_RIGHT_1=186,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.053
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=161
PRIMER_PAIR_PENALTY_2=0.1168
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_RIGHT_2_SEQUENCE=AAGCTTCAGGATTCTCAACTGG
PRIMER_LEFT_2=26,22
PRIMER_RIGHT_2=199,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.1293
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.019805
PRIMER_LEFT_3_SEQUENCE=ATCGGTTGAAGAAGTGAAGGAA
PRIMER_RIGHT_3_SEQUENCE=ACACCCATTCCATTGGTTTAAG
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=218,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.980
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=0.1293
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.019805
PRIMER_LEFT_4_SEQUENCE=AATCGGTTGAAGAAGTGAAGGA
PRIMER_RIGHT_4_SEQUENCE=ACACCCATTCCATTGGTTTAAG
PRIMER_LEFT_4=20,22
PRIMER_RIGHT_4=218,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_02106 Ps_contig_mira-and-tgicl-ass_franssen_18910
SEQUENCE=CTTGTTTATAAACCTCATTTTCTGGGTCACCTCCGCTTTCAGTGACCTTTTTATCATCAACTTTATGAGCTCCAAGATCAGTCATTTGATCATCATCATCACCTTTGTCATCCTCAGTTTTAACGCTAGCACCAGCCAACACACGGACTTCATGTTGCCGCCGGCGATTAAGAATACGCTCAAGTTCTCCGCATGTTAGGTAACGGTAGATGCGAAGCTATGTGCATCGACGGTACAAAGCGTCTCTGTCACATCCGAGGGAAGATGCAACTAAAAAAGGTTCTTGGTTATTTT
TARGET=89,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4203
PRIMER_LEFT_PENALTY=0.333229
PRIMER_RIGHT_PENALTY=0.087031
PRIMER_LEFT_SEQUENCE=CCGCTTTCAGTGACCTTTTTAT
PRIMER_RIGHT_SEQUENCE=CGGAGAACTTGAGCGTATTCTT
PRIMER_LEFT=32,22
PRIMER_RIGHT=190,22
PRIMER_LEFT_TM=59.667
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=159
PRIMER_PAIR_PENALTY_1=0.5094
PRIMER_LEFT_1_PENALTY=0.333229
PRIMER_RIGHT_1_PENALTY=0.176180
PRIMER_LEFT_1_SEQUENCE=CCGCTTTCAGTGACCTTTTTAT
PRIMER_RIGHT_1_SEQUENCE=ACATAGCTTCGCATCTACCGTT
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=222,22
PRIMER_LEFT_1_TM=59.667
PRIMER_RIGHT_1_TM=60.176
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.5724
PRIMER_LEFT_2_PENALTY=0.333229
PRIMER_RIGHT_2_PENALTY=0.239177
PRIMER_LEFT_2_SEQUENCE=CCGCTTTCAGTGACCTTTTTAT
PRIMER_RIGHT_2_SEQUENCE=CTTGAGCGTATTCTTAATCGCC
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=183,22
PRIMER_LEFT_2_TM=59.667
PRIMER_RIGHT_2_TM=60.239
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.6412
PRIMER_LEFT_3_PENALTY=0.333229
PRIMER_RIGHT_3_PENALTY=0.307953
PRIMER_LEFT_3_SEQUENCE=CCGCTTTCAGTGACCTTTTTAT
PRIMER_RIGHT_3_SEQUENCE=GGTGCTAGCGTTAAAACTGAGG
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=132,22
PRIMER_LEFT_3_TM=59.667
PRIMER_RIGHT_3_TM=60.308
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=0.7368
PRIMER_LEFT_4_PENALTY=0.333229
PRIMER_RIGHT_4_PENALTY=0.403587
PRIMER_LEFT_4_SEQUENCE=CCGCTTTCAGTGACCTTTTTAT
PRIMER_RIGHT_4_SEQUENCE=CTGGTGCTAGCGTTAAAACTGA
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=134,22
PRIMER_LEFT_4_TM=59.667
PRIMER_RIGHT_4_TM=59.596
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_02107 Ps_contig_mira-and-tgicl-ass_franssen_18913
SEQUENCE=GTGGGGTTTTAGTTTTTTAATTTTGAAATTCGATCATTATCTTTTAACTATTTCCCTTTTTGTATGTAAAGGATGTTGTTGTAAGTGTAACGTGTTGTTGTAATGATTATTATTATTAGGTGGTGAGTGCGTTGGGTGATGGGGCAGTTTTTTCATTACTTTTAAGTTGGAATCTGGCCCAGCCCATGTGTGATGTATGGTTGTAATTGTGTAAACTTTAAATTAATAAAAATAATGATATTCTCTTTTGGCATACAAA
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.5871
PRIMER_LEFT_PENALTY=3.453078
PRIMER_RIGHT_PENALTY=1.134049
PRIMER_LEFT_SEQUENCE=GGATGTTGTTGTAAGTGTAACGTG
PRIMER_RIGHT_SEQUENCE=ACCATACATCACACATGGGCT
PRIMER_LEFT=70,24
PRIMER_RIGHT=200,21
PRIMER_LEFT_TM=58.547
PRIMER_RIGHT_TM=60.134
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=131
PRIMER_PAIR_PENALTY_1=4.7989
PRIMER_LEFT_1_PENALTY=3.664847
PRIMER_RIGHT_1_PENALTY=1.134049
PRIMER_LEFT_1_SEQUENCE=GGATGTTGTTGTAAGTGTAACGTGT
PRIMER_RIGHT_1_SEQUENCE=ACCATACATCACACATGGGCT
PRIMER_LEFT_1=70,25
PRIMER_RIGHT_1=200,21
PRIMER_LEFT_1_TM=59.335
PRIMER_RIGHT_1_TM=60.134
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=4.8912
PRIMER_LEFT_2_PENALTY=3.453078
PRIMER_RIGHT_2_PENALTY=1.438091
PRIMER_LEFT_2_SEQUENCE=GGATGTTGTTGTAAGTGTAACGTG
PRIMER_RIGHT_2_SEQUENCE=AACCATACATCACACATGGGCT
PRIMER_LEFT_2=70,24
PRIMER_RIGHT_2=201,22
PRIMER_LEFT_2_TM=58.547
PRIMER_RIGHT_2_TM=61.438
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=4.9729
PRIMER_LEFT_3_PENALTY=3.453078
PRIMER_RIGHT_3_PENALTY=1.519775
PRIMER_LEFT_3_SEQUENCE=GGATGTTGTTGTAAGTGTAACGTG
PRIMER_RIGHT_3_SEQUENCE=AACCATACATCACACATGGGC
PRIMER_LEFT_3=70,24
PRIMER_RIGHT_3=201,21
PRIMER_LEFT_3_TM=58.547
PRIMER_RIGHT_3_TM=60.520
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=4.9805
PRIMER_LEFT_4_PENALTY=3.542384
PRIMER_RIGHT_4_PENALTY=1.438091
PRIMER_LEFT_4_SEQUENCE=AGGATGTTGTTGTAAGTGTAACGTG
PRIMER_RIGHT_4_SEQUENCE=AACCATACATCACACATGGGCT
PRIMER_LEFT_4=69,25
PRIMER_RIGHT_4=201,22
PRIMER_LEFT_4_TM=59.458
PRIMER_RIGHT_4_TM=61.438
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_02108 Ps_contig_mira-and-tgicl-ass_franssen_18914
SEQUENCE=ATCGGTGGAGGAGAAGATTTATATTTGTAAATTGGTGGGGAATAGACCGGTGGAGGAGGTGAAGAGTAGACATAGTGTGGTGGAGGTGGTGAGTAAACAGGTGGAGGAGGAGATTTATATTTGTAAACGGGTGGTGGCGAGTAAACAGGTGGAGGAGATTTATATTTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTGACTTGTACTTGTAAACTGGWGGTGGAGGAGGTGAGTGTACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATGAATAGATGTAGTTGTTTGCTTGAGATGGCAAAGTGAGAGAAAGTAATGTGAATGCAATAGTGAGAGTAATAGAGGCCATTTGAGACCCCATGTTTTCTCAAAGTAGATTTTSTTTTCGCCCCCGNGTGCTCTGCGTTGACTACCCNCTGCTT
TARGET=249,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02109 Ps_contig_mira-and-tgicl-ass_franssen_18914
SEQUENCE=ATCGGTGGAGGAGAAGATTTATATTTGTAAATTGGTGGGGAATAGACCGGTGGAGGAGGTGAAGAGTAGACATAGTGTGGTGGAGGTGGTGAGTAAACAGGTGGAGGAGGAGATTTATATTTGTAAACGGGTGGTGGCGAGTAAACAGGTGGAGGAGATTTATATTTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTGACTTGTACTTGTAAACTGGWGGTGGAGGAGGTGAGTGTACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATGAATAGATGTAGTTGTTTGCTTGAGATGGCAAAGTGAGAGAAAGTAATGTGAATGCAATAGTGAGAGTAATAGAGGCCATTTGAGACCCCATGTTTTCTCAAAGTAGATTTTSTTTTCGCCCCCGNGTGCTCTGCGTTGACTACCCNCTGCTT
TARGET=280,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02110 Ps_contig_mira-and-tgicl-ass_franssen_18914
SEQUENCE=ATCGGTGGAGGAGAAGATTTATATTTGTAAATTGGTGGGGAATAGACCGGTGGAGGAGGTGAAGAGTAGACATAGTGTGGTGGAGGTGGTGAGTAAACAGGTGGAGGAGGAGATTTATATTTGTAAACGGGTGGTGGCGAGTAAACAGGTGGAGGAGATTTATATTTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTGACTTGTACTTGTAAACTGGWGGTGGAGGAGGTGAGTGTACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATGAATAGATGTAGTTGTTTGCTTGAGATGGCAAAGTGAGAGAAAGTAATGTGAATGCAATAGTGAGAGTAATAGAGGCCATTTGAGACCCCATGTTTTCTCAAAGTAGATTTTSTTTTCGCCCCCGNGTGCTCTGCGTTGACTACCCNCTGCTT
TARGET=414,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02111 Ps_contig_mira-and-tgicl-ass_franssen_18917
SEQUENCE=GAATGTAAGCTGTACAGATTGGCAATTTCCCTACTTTTTCTTATTTGTGATTCTTGAACGCAGCACAAATTACCAATTATTCCAAGTATTAACGCAATCGCGTGGAGAACAAGGTGTATCAATTTCTTCACTTCCTTCTTCAAGGGAAGAGATTTGTAACTTATAATCGCTTCACCTCCAACTATTATTAAGCCAATAAGCATAAGAACAGGATGAAGATTGAAGATGAGAGCCTTGTTGGTAGAACTCCAAGCCAAACCACCTMTGAAATGGAAGTTCCAGACCAACACCAACACGGCGGCGCAACACCGAGAAGGTGACAACGTACGTGAACGGAACGGCGGGTCACCCACNCCNTCTTCAACGTATTGGTGTTGTTGTTGTTGGGTAGAAGA
TARGET=373,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02112 Ps_contig_mira-and-tgicl-ass_franssen_18927
SEQUENCE=TAGGAAACTAGTTGTATGCATTTTAACTAAACCAAAGTAGTTGGCGTATCAAGATTATGGTGAGGCTGACAATCAAAACTAGGTGCACAAATTACCAATGGAGGTTACACTAATCTTAGTTAACAATGGAACCAAACATGCATTCAACAACACTGCAGGAACTCAGAATTTTGGAAAGGCCAAGATCAACACTGGAGCCAGGATTGGAAATTAAATCATTACTATCAGCTTTGATGGGCTTTCCAAACATATACATATAATATAATATAATAAGTGTATGTGTATGTTTTGGGTAATAATAGTAATAAAATAAAGTGTTTTAWTCTCTATCTTATGAAGTGTTGTTTTGTGTAAGTAAAAATAAATAATAATAAATTGGTAGTAAGTTT
TARGET=255,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02113 Ps_contig_mira-and-tgicl-ass_franssen_18932
SEQUENCE=GGCCGGATAATTTGGAAAACCTGAAGAAGATAGCTTTTTGTTTAGTTCAGTTCAATAAGCAGGTTCCTGAAGCAGGTGCTGGCACAGCCACAGCACAAGAAGAGAATGATGATGATGATGTTCCAGAGCTTGTCCCAGGAGAGACCTTTGAGACAGCTGCTGAGGAGGCCAAGGATAGTTAGTGTTATTGTTGTTTTTTTTCAACAAAGAAAATCTTGTATTTTCTAATTTTAAGTTGTTTTAGGTTGTTTTGTCCTTCTTGTCATATTAHTTAAACAGTCTCWAWTACTTAGTAATTTAAATTACTTYGTTTTTCCTTTAGTTAMWAAA
TARGET=106,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02114 Ps_contig_mira-and-tgicl-ass_franssen_18942
SEQUENCE=TGGGGAATGGTAGACAGGGTGAGGATGGGGATTTAGGTTTGTTGTCTACTTGGTTGGKTGGTGGAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGAGGAGGAGAGTGGTAGACTGGGTGTGGGTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATTCCGTTTTGGGTGGGCGGGTGGTGAGGTGGACCGGTGGTGGTGGTGAGGAATAAGAATTATTTGGGTTTGCTGATTATTTCAGAGTAGGGAAACTARRAAAAAGAGTAGTGGACTAA
TARGET=271,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02115 Ps_contig_mira-and-tgicl-ass_franssen_18946
SEQUENCE=CCAAGCAGGGATCGGATAGTTTACTTCACTGCTTGTGCATTTAGKWARAAAWTTGAGGGATACGTTCTTCAATGGCGTTAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTTGTGTACACTTCTACCAGAAAGATATGGTTGTTGTTTACTTGTAACATTTTCATCATCTTTGCTTGAATTGTAACTCAAAAGTCTTAGCTTGTTTTGTTAGCTTGAGTTCTTTGTAAATCACTTCAGTTTAGTGTTTCAACAATTAATTAATTTTATCATGAAATTTAAAAAAAAACCTTT
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02116 Ps_contig_mira-and-tgicl-ass_franssen_18946
SEQUENCE=CCAAGCAGGGATCGGATAGTTTACTTCACTGCTTGTGCATTTAGKWARAAAWTTGAGGGATACGTTCTTCAATGGCGTTAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTTGTGTACACTTCTACCAGAAAGATATGGTTGTTGTTTACTTGTAACATTTTCATCATCTTTGCTTGAATTGTAACTCAAAAGTCTTAGCTTGTTTTGTTAGCTTGAGTTCTTTGTAAATCACTTCAGTTTAGTGTTTCAACAATTAATTAATTTTATCATGAAATTTAAAAAAAAACCTTT
TARGET=345,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02117 Ps_contig_mira-and-tgicl-ass_franssen_18951
SEQUENCE=AACATGAAACAAAATAAAAACGAACTAACTACAGAATTTAAAATTACAAACAAGANCCGTCCGTCCCGTCCAATGAAAAGTACAGATCAAGAATCCACGTGGACGAGTCCTAGAAAACGAATGGCAAGAAAGCAGCAGAGAGAGCAGTTGCAGCAACAATGGGTTTTTGTCTTGAAGGAAAATGCTGCCGAGGGAGAAGGTGGAGAATCGGTTGGTGAAGAGGCGGGACCACTTTCTGGGGAGGTTGGAGTAGGTGCGGGAGGAGAGGCAGAAGAGGCAGGTCCACCGGTTGGTGTTGGAGCTGGGAGGGGAGGAAGCTGGAGAAGAGGCGGGTGCTGGAGTGGCTGGAGGTGGGAGCAGCTGCAGGTGGTGTTGCTGCGTGGGAGGTAGTTGCGTAGGTTTGGGTGGTAGTTGGCGTAGGGTTGGGTTGGTTGGTTGGCGGTTGGGGTGGGTCGGTTGGTTCGGCCGGGTGCGTAGGGTAGTTTAGGGG
TARGET=426,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3277
PRIMER_LEFT_PENALTY=1.227324
PRIMER_RIGHT_PENALTY=1.100425
PRIMER_LEFT_SEQUENCE=ACTTTCTGGGGAGGTTGGAGTA
PRIMER_RIGHT_SEQUENCE=CCCCTAAACTACCCTACGCAC
PRIMER_LEFT=230,22
PRIMER_RIGHT=489,21
PRIMER_LEFT_TM=61.227
PRIMER_RIGHT_TM=59.900
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=57.143
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=2.5735
PRIMER_LEFT_1_PENALTY=1.473077
PRIMER_RIGHT_1_PENALTY=1.100425
PRIMER_LEFT_1_SEQUENCE=CTTTCTGGGGAGGTTGGAGTA
PRIMER_RIGHT_1_SEQUENCE=CCCCTAAACTACCCTACGCAC
PRIMER_LEFT_1=231,21
PRIMER_RIGHT_1=489,21
PRIMER_LEFT_1_TM=60.473
PRIMER_RIGHT_1_TM=59.900
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=57.143
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=3.1037
PRIMER_LEFT_2_PENALTY=2.003268
PRIMER_RIGHT_2_PENALTY=1.100425
PRIMER_LEFT_2_SEQUENCE=GGAGAATCGGTTGGTGAAGAG
PRIMER_RIGHT_2_SEQUENCE=CCCCTAAACTACCCTACGCAC
PRIMER_LEFT_2=201,21
PRIMER_RIGHT_2=489,21
PRIMER_LEFT_2_TM=61.003
PRIMER_RIGHT_2_TM=59.900
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=57.143
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=3.1037
PRIMER_LEFT_3_PENALTY=2.003268
PRIMER_RIGHT_3_PENALTY=1.100425
PRIMER_LEFT_3_SEQUENCE=GGAGAATCGGTTGGTGAAGAG
PRIMER_RIGHT_3_SEQUENCE=CCTAAACTACCCTACGCACCC
PRIMER_LEFT_3=201,21
PRIMER_RIGHT_3=487,21
PRIMER_LEFT_3_TM=61.003
PRIMER_RIGHT_3_TM=59.900
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=57.143
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=3.1037
PRIMER_LEFT_4_PENALTY=2.003268
PRIMER_RIGHT_4_PENALTY=1.100425
PRIMER_LEFT_4_SEQUENCE=GAGAAGGTGGAGAATCGGTTG
PRIMER_RIGHT_4_SEQUENCE=CCCCTAAACTACCCTACGCAC
PRIMER_LEFT_4=193,21
PRIMER_RIGHT_4=489,21
PRIMER_LEFT_4_TM=61.003
PRIMER_RIGHT_4_TM=59.900
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_02118 Ps_contig_mira-and-tgicl-ass_franssen_18961
SEQUENCE=AAGAGTACGAACCGAGAATGAGCGATTGCTTAGGGTTCAGNTCCGGCCTCGAACCACTGGAACTCCACTCCATGCCTGACCACAAAGAATTCCTAGAACTGCAGCCATTGCCCATCTTCCATGAATGAGCTCAGCTTCTCTGTACCATTTGAGAAATGCTTTGGGTTCCTTGCCAAGGCCTAGTGGGTCGAAACCAAAGTCACCCGGTAGCGATCCGTCGAGCCATTCGGGGTCGACGAGGTTTCCACCGAAACTAACACCAGGGATCCATGATTTCTTAGGTGCAGAAGCAGCAGCAGCAACAATAAGTCTTTTAGGACTAACAGCTGCAACACCAACTTTGCTTCCAATGGCAGTAGCTAGCAGGTTGCTCTCTTCCACAGATAAGAAGGAAGACTACCCCAAACCATTCCAACACAGCAACCAGATT
TARGET=289,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1418
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.108707
PRIMER_LEFT_SEQUENCE=CTAGTGGGTCGAAACCAAAGTC
PRIMER_RIGHT_SEQUENCE=ATGGTTTGGGGTAGTCTTCCTT
PRIMER_LEFT=179,22
PRIMER_RIGHT=409,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.1418
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.108707
PRIMER_LEFT_1_SEQUENCE=CTAGTGGGTCGAAACCAAAGTC
PRIMER_RIGHT_1_SEQUENCE=AATGGTTTGGGGTAGTCTTCCT
PRIMER_LEFT_1=179,22
PRIMER_RIGHT_1=410,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.1589
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.125755
PRIMER_LEFT_2_SEQUENCE=CTAGTGGGTCGAAACCAAAGTC
PRIMER_RIGHT_2_SEQUENCE=GGTTTGGGGTAGTCTTCCTTCT
PRIMER_LEFT_2=179,22
PRIMER_RIGHT_2=407,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.874
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=0.1851
PRIMER_LEFT_3_PENALTY=0.033137
PRIMER_RIGHT_3_PENALTY=0.151991
PRIMER_LEFT_3_SEQUENCE=CTAGTGGGTCGAAACCAAAGTC
PRIMER_RIGHT_3_SEQUENCE=GTTGGTGTTGCAGCTGTTAGTC
PRIMER_LEFT_3=179,22
PRIMER_RIGHT_3=338,22
PRIMER_LEFT_3_TM=60.033
PRIMER_RIGHT_3_TM=59.848
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=0.3041
PRIMER_LEFT_4_PENALTY=0.195430
PRIMER_RIGHT_4_PENALTY=0.108707
PRIMER_LEFT_4_SEQUENCE=GGATCCATGATTTCTTAGGTGC
PRIMER_RIGHT_4_SEQUENCE=ATGGTTTGGGGTAGTCTTCCTT
PRIMER_LEFT_4=263,22
PRIMER_RIGHT_4=409,22
PRIMER_LEFT_4_TM=59.805
PRIMER_RIGHT_4_TM=60.109
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_02119 Ps_contig_mira-and-tgicl-ass_franssen_18962
SEQUENCE=GGAGCAATCCATTTTGTAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGKAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGAGGAGGGTTTGAATGTCTTTAG
TARGET=143,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02120 Ps_contig_mira-and-tgicl-ass_franssen_18962
SEQUENCE=GGAGCAATCCATTTTGTAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGKAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGAGGAGGGTTTGAATGTCTTTAG
TARGET=196,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02121 Ps_contig_mira-and-tgicl-ass_franssen_18962
SEQUENCE=GGAGCAATCCATTTTGTAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGKAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGAGGAGGGTTTGAATGTCTTTAG
TARGET=217,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02122 Ps_contig_mira-and-tgicl-ass_franssen_18962
SEQUENCE=GGAGCAATCCATTTTGTAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGKAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGAGGAGGGTTTGAATGTCTTTAG
TARGET=231,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02123 Ps_contig_mira-and-tgicl-ass_franssen_18962
SEQUENCE=GGAGCAATCCATTTTGTAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGKAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGAGGAGGGTTTGAATGTCTTTAG
TARGET=307,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02124 Ps_contig_mira-and-tgicl-ass_franssen_18973
SEQUENCE=GAACGAGTGTTCATTCAGTGCTATATAAGGGSACCTAGGGGAGTATAGATTTCACACAAAAAAAMACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGACCCTTTAACCAAAGAAGAGTTTCTAGCTGTTCAAACCATAGTTTACCAAAAACAATACCCCATCTACAAAGAACAAACTACG
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02125 Ps_contig_mira-and-tgicl-ass_franssen_18973
SEQUENCE=GAACGAGTGTTCATTCAGTGCTATATAAGGGSACCTAGGGGAGTATAGATTTCACACAAAAAAAMACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGACCCTTTAACCAAAGAAGAGTTTCTAGCTGTTCAAACCATAGTTTACCAAAAACAATACCCCATCTACAAAGAACAAACTACG
TARGET=112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02126 Ps_contig_mira-and-tgicl-ass_franssen_18974
SEQUENCE=GAAAACAACAACATTATCTCAAGTTTCATTGACGGCGATGGCTGATTCTTCTGATGATACATCTACTTCTCTCGATACCAATGAGTTGTTTTACCGATTTGAAGGAGAAGTGGGATGGTCTTGAAAACAAGTCCACAGTGCTTCTGTATGGTGGTGGTGGTTTGGTTGCTGTTTGGTTATCTTCAATTCTTGTAGGTGCCATCAACTCAGTCCCCTTGCTTCCAAAGATTATGGAGTTAGGTAAGGAAACTAAAGGGTAACACTGGATGGTTTGTCTACCGGTACCTTCTGTT
TARGET=149,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0271
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=AGAAGTGGGATGGTCTTGAAAA
PRIMER_RIGHT_SEQUENCE=TTGATGGCACCTACAAGAATTG
PRIMER_LEFT=105,22
PRIMER_RIGHT=204,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=0.0635
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=GATTTGAAGGAGAAGTGGGATG
PRIMER_RIGHT_1_SEQUENCE=TTGATGGCACCTACAAGAATTG
PRIMER_LEFT_1=95,22
PRIMER_RIGHT_1=204,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=110
PRIMER_PAIR_PENALTY_2=0.1675
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.143968
PRIMER_LEFT_2_SEQUENCE=AGAAGTGGGATGGTCTTGAAAA
PRIMER_RIGHT_2_SEQUENCE=CAAACCATCCAGTGTTACCCTT
PRIMER_LEFT_2=105,22
PRIMER_RIGHT_2=273,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.144
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.2023
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.178718
PRIMER_LEFT_3_SEQUENCE=AGAAGTGGGATGGTCTTGAAAA
PRIMER_RIGHT_3_SEQUENCE=GACTGAGTTGATGGCACCTACA
PRIMER_LEFT_3=105,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=60.179
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=0.2269
PRIMER_LEFT_4_PENALTY=0.023556
PRIMER_RIGHT_4_PENALTY=0.203324
PRIMER_LEFT_4_SEQUENCE=AGAAGTGGGATGGTCTTGAAAA
PRIMER_RIGHT_4_SEQUENCE=CTGAGTTGATGGCACCTACAAG
PRIMER_LEFT_4=105,22
PRIMER_RIGHT_4=209,22
PRIMER_LEFT_4_TM=59.976
PRIMER_RIGHT_4_TM=59.797
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_02127 Ps_contig_mira-and-tgicl-ass_franssen_18975
SEQUENCE=CCTACCCCAACCAACCCAACCTTTTCGTCGAGTACCAATACGCAGCTCCCACTAACATGAGAAGACGAGAGCAGCTGCGGCTCCAAGGAAAGACCAAAAAACGGTGCCGTTTCATCGCCGCTGAATGGAGCCATTCCTACCGAAACGACGTCGAATAGATCCGATCTAAGATAAACGGTTTTACGATTTTTCAGAGAGAGAGAAAGAAGTTCGAATTTCCGATCTCTATTGCCGTAGAGAAAAATGATTCCCCGCGTACTCTGCGTTGA
TARGET=193,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0922
PRIMER_LEFT_PENALTY=0.048131
PRIMER_RIGHT_PENALTY=0.044097
PRIMER_LEFT_SEQUENCE=AATACGCAGCTCCCACTAACAT
PRIMER_RIGHT_SEQUENCE=TACGGCAATAGAGATCGGAAAT
PRIMER_LEFT=36,22
PRIMER_RIGHT=235,22
PRIMER_LEFT_TM=60.048
PRIMER_RIGHT_TM=59.956
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=200
PRIMER_PAIR_PENALTY_1=0.3098
PRIMER_LEFT_1_PENALTY=0.265715
PRIMER_RIGHT_1_PENALTY=0.044097
PRIMER_LEFT_1_SEQUENCE=TCCCACTAACATGAGAAGACGA
PRIMER_RIGHT_1_SEQUENCE=TACGGCAATAGAGATCGGAAAT
PRIMER_LEFT_1=46,22
PRIMER_RIGHT_1=235,22
PRIMER_LEFT_1_TM=59.734
PRIMER_RIGHT_1_TM=59.956
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.4877
PRIMER_LEFT_2_PENALTY=0.048131
PRIMER_RIGHT_2_PENALTY=0.439553
PRIMER_LEFT_2_SEQUENCE=AATACGCAGCTCCCACTAACAT
PRIMER_RIGHT_2_SEQUENCE=GGAATCATTTTTCTCTACGGCA
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=250,22
PRIMER_LEFT_2_TM=60.048
PRIMER_RIGHT_2_TM=60.440
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=11.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=0.6247
PRIMER_LEFT_3_PENALTY=0.048131
PRIMER_RIGHT_3_PENALTY=0.576589
PRIMER_LEFT_3_SEQUENCE=AATACGCAGCTCCCACTAACAT
PRIMER_RIGHT_3_SEQUENCE=CTACGGCAATAGAGATCGGAAA
PRIMER_LEFT_3=36,22
PRIMER_RIGHT_3=236,22
PRIMER_LEFT_3_TM=60.048
PRIMER_RIGHT_3_TM=60.577
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=0.7053
PRIMER_LEFT_4_PENALTY=0.265715
PRIMER_RIGHT_4_PENALTY=0.439553
PRIMER_LEFT_4_SEQUENCE=TCCCACTAACATGAGAAGACGA
PRIMER_RIGHT_4_SEQUENCE=GGAATCATTTTTCTCTACGGCA
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=250,22
PRIMER_LEFT_4_TM=59.734
PRIMER_RIGHT_4_TM=60.440
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=11.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_02128 Ps_contig_mira-and-tgicl-ass_franssen_18981
SEQUENCE=GACCGTAACCACCACCACCGCCATGACCGTAACCTCCTACCTACCATGCTCTGGAAGACACTCACTCATCATGAGTGGTTGTCTCAATTTGACTTCTGTACATTGTGCTTGTTGGCTATTCATGCACACCGAGGAATCMAAAACCTTCCCCTCATACTGCTCATTAATAAACCACATGACTTCGAGGAAGTGCCTTTGATGGGCTCAGAGAAGTGTGCGATGCTGCTATCCTTTCCTTCCCCCTCCCCTGTGGTGTAGATACGTTTGTGTTGGAGACTTGAGCTACAAATGAACAGCCTATTTGTTGTTGTGTTTCATTAATTCTTTTGTTTTTATTTACTTCCCCCAAACTATGGTAGTTTTATGTATTGGTTTTGGATWGAACYAAAAAAACTAGTCTTGTTTCCTTAGGGTTTACGC
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02129 Ps_contig_mira-and-tgicl-ass_franssen_18988
SEQUENCE=GTCRGGGATCTTTCTTCATATCTCTCTCACTTTCACTCCCAACCAAAACACACACACATCATGGGTTTGCAGGAGGTATTTTGAGGAGCATGCTGACGAAAAGTTAAGACTACTAACAGAGAGTCCATACAAATAGTAAAACTTGTCTTTACTTCCTTTAGTGGTATTA
TARGET=48,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02130 Ps_contig_mira-and-tgicl-ass_franssen_18989
SEQUENCE=GATCCACAACCTTAAAACCCCATCCTTACTCTCCCCTTTCCCTWRRYATCWWYKCAGAGTCGCGGAAGTTTTCTCTTCATCGGACTAGAGCTTCTTCTTCTGATGATACATCTACTTCTCTCGATACCAATGAGTTGTTTACCGATTTGAAGGAGAAGTGGGATGGTCTTAGAAAACAAGTCCACAGTGCTTCTGYATGGTGGTGGTGGTTTGGTTGCTTGTTTGGTTATCTTAACAATTCTTGTAGGTGCCATACAACTCACGTTCCCCTTGCCTTACCAAAG
TARGET=198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02131 Ps_contig_mira-and-tgicl-ass_franssen_18998
SEQUENCE=GTACTAAATGGATTCCAGAAAAGACAATGCTCATTCTTGGCCTATTTGGCCATTGGGTTCTTCTTTATTTTCTATTCTAGAGGTGTCAGCTAGGGAATTAACTGAGGAGGTTGTTGAAAAGTCAGATGAAGTAAACGATGCTAAATRTGKMGGKTATGGACGTGGTGGTGGTTACCATAATGGAGGCGGTTACCACAATGGTGGAGGAGGATACAATGGCGGTGGCGGTTACCACAATGGTGGAGGAGGATACAATGGCGGTGGCGGTTACCACAATGGTGGAGGTGGTTACCATAATGGTGGAGGTGGTTACAATGGAACGGTGGAGGTTACCATAATGGTGGTGGTGGTTACCATGGTGGAGGTGGACACGGTGGACATGGTGGTGCTTACCAACAACGG
TARGET=339,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02132 Ps_contig_mira-and-tgicl-ass_franssen_19002
SEQUENCE=CAATGGTGGTGGTAAGTTACCATGGTGGNGCGGCAGTTACCAGGTGGACATGGAGACATGGTGACATGGTGGACATGGTGGTGTTTCCAACAATGAGGTTGTTAGAAAACTTACAAATGAACGTAAATGATGACCAAATATGGACACTACGGTCATGGTGGTGGTGGTTACCATGGTGGTGGTGGCGGTTACCATGGTGGACATGGTGGACACGGTGGTGCTTCAGACAATGGAAACTGATTATATCATCATACATGATGCACATTCAAAGTAATAAGTTTCATGAATGAATAGTTTGGTGTAAATAAAAGAGGTACAAATAGTCATCATTATGTTGTGGTTATTATGTATTATTTTGTATATGGTGACGAGTTTTATTTCGAGTGAAAGTCATGAATGTGTTATTTA
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4734
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=0.219884
PRIMER_LEFT_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT=133,22
PRIMER_RIGHT=238,22
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=59.780
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=0.4734
PRIMER_LEFT_1_PENALTY=0.253482
PRIMER_RIGHT_1_PENALTY=0.219884
PRIMER_LEFT_1_SEQUENCE=TGACCAAATATGGACACTACGG
PRIMER_RIGHT_1_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT_1=130,22
PRIMER_RIGHT_1=238,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=59.780
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.7905
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.536975
PRIMER_LEFT_2_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_2_SEQUENCE=AGTTTCCATTGTCTGAAGCACC
PRIMER_LEFT_2=133,22
PRIMER_RIGHT_2=237,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.537
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=0.7905
PRIMER_LEFT_3_PENALTY=0.253482
PRIMER_RIGHT_3_PENALTY=0.536975
PRIMER_LEFT_3_SEQUENCE=TGACCAAATATGGACACTACGG
PRIMER_RIGHT_3_SEQUENCE=AGTTTCCATTGTCTGAAGCACC
PRIMER_LEFT_3=130,22
PRIMER_RIGHT_3=237,22
PRIMER_LEFT_3_TM=59.747
PRIMER_RIGHT_3_TM=60.537
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=108
PRIMER_PAIR_PENALTY_4=0.9866
PRIMER_LEFT_4_PENALTY=0.253482
PRIMER_RIGHT_4_PENALTY=0.733091
PRIMER_LEFT_4_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_4_SEQUENCE=CTCGAAATAAAACTCGTCACCA
PRIMER_LEFT_4=133,22
PRIMER_RIGHT_4=383,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=59.267
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_02133 Ps_contig_mira-and-tgicl-ass_franssen_19002
SEQUENCE=CAATGGTGGTGGTAAGTTACCATGGTGGNGCGGCAGTTACCAGGTGGACATGGAGACATGGTGACATGGTGGACATGGTGGTGTTTCCAACAATGAGGTTGTTAGAAAACTTACAAATGAACGTAAATGATGACCAAATATGGACACTACGGTCATGGTGGTGGTGGTTACCATGGTGGTGGTGGCGGTTACCATGGTGGACATGGTGGACACGGTGGTGCTTCAGACAATGGAAACTGATTATATCATCATACATGATGCACATTCAAAGTAATAAGTTTCATGAATGAATAGTTTGGTGTAAATAAAAGAGGTACAAATAGTCATCATTATGTTGTGGTTATTATGTATTATTTTGTATATGGTGACGAGTTTTATTTCGAGTGAAAGTCATGAATGTGTTATTTA
TARGET=174,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4734
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=0.219884
PRIMER_LEFT_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT=133,22
PRIMER_RIGHT=238,22
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=59.780
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=0.4734
PRIMER_LEFT_1_PENALTY=0.253482
PRIMER_RIGHT_1_PENALTY=0.219884
PRIMER_LEFT_1_SEQUENCE=TGACCAAATATGGACACTACGG
PRIMER_RIGHT_1_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT_1=130,22
PRIMER_RIGHT_1=238,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=59.780
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.7905
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.536975
PRIMER_LEFT_2_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_2_SEQUENCE=AGTTTCCATTGTCTGAAGCACC
PRIMER_LEFT_2=133,22
PRIMER_RIGHT_2=237,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.537
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=0.7905
PRIMER_LEFT_3_PENALTY=0.253482
PRIMER_RIGHT_3_PENALTY=0.536975
PRIMER_LEFT_3_SEQUENCE=TGACCAAATATGGACACTACGG
PRIMER_RIGHT_3_SEQUENCE=AGTTTCCATTGTCTGAAGCACC
PRIMER_LEFT_3=130,22
PRIMER_RIGHT_3=237,22
PRIMER_LEFT_3_TM=59.747
PRIMER_RIGHT_3_TM=60.537
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=108
PRIMER_PAIR_PENALTY_4=0.9866
PRIMER_LEFT_4_PENALTY=0.253482
PRIMER_RIGHT_4_PENALTY=0.733091
PRIMER_LEFT_4_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_4_SEQUENCE=CTCGAAATAAAACTCGTCACCA
PRIMER_LEFT_4=133,22
PRIMER_RIGHT_4=383,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=59.267
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_02134 Ps_contig_mira-and-tgicl-ass_franssen_19007
SEQUENCE=GACTATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCCAAACCCCCAAAACATGGAAACCAACCCTCCACTCTATCTTTCATCTCTCTCAAACCAACCAGTTCACACCAAGAACAACAAAAATTGCTGCAACAGAAGACAAAACCGATGCACGACAACCGATGTAGACAAACAAAAGAAGCACCAGTTGGATTCACCCCACCTGAGCT
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02135 Ps_contig_mira-and-tgicl-ass_franssen_19008
SEQUENCE=AAAGGGTGTGGAAAGTGATGATGGCATTAGCATTGATGATAATTGGTGGATGAGTGATGATACATTATATGGGGATAGAAAGAAGGAAAAGAGAAGAAGTAGGAATAATCGTGGTAGTGTTGATTGGTGGTTAGACGGGTTCAGTGGCGAGTTATGGAGAGCGCGTAGGAATAGTTTTGATTCTGCTAGTGGAGACATTCCAAAGAGTGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCCGGGATCGTGGGTATGGAGGAAGTGGTGGAGGAGGCGGTTATTCTCGTGGAGGAGGCGGTGGCGGTGGAAACTGGAGGTGGTTACCATAATGGTGGAGGTGGTTACAA
TARGET=212,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5359
PRIMER_LEFT_PENALTY=0.061436
PRIMER_RIGHT_PENALTY=0.474473
PRIMER_LEFT_SEQUENCE=AGAGCGCGTAGGAATAGTTTTG
PRIMER_RIGHT_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT=157,22
PRIMER_RIGHT=363,22
PRIMER_LEFT_TM=59.939
PRIMER_RIGHT_TM=60.474
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.5991
PRIMER_LEFT_1_PENALTY=0.124655
PRIMER_RIGHT_1_PENALTY=0.474473
PRIMER_LEFT_1_SEQUENCE=TCTGCTAGTGGAGACATTCCAA
PRIMER_RIGHT_1_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT_1=181,22
PRIMER_RIGHT_1=363,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.474
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.6592
PRIMER_LEFT_2_PENALTY=0.011696
PRIMER_RIGHT_2_PENALTY=0.647458
PRIMER_LEFT_2_SEQUENCE=AGTGATGATGGCATTAGCATTG
PRIMER_RIGHT_2_SEQUENCE=ATAACCACCACCACCATATCCT
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=261,22
PRIMER_LEFT_2_TM=59.988
PRIMER_RIGHT_2_TM=59.353
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.6876
PRIMER_LEFT_3_PENALTY=0.040146
PRIMER_RIGHT_3_PENALTY=0.647458
PRIMER_LEFT_3_SEQUENCE=TGGAAAGTGATGATGGCATTAG
PRIMER_RIGHT_3_SEQUENCE=ATAACCACCACCACCATATCCT
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=59.960
PRIMER_RIGHT_3_TM=59.353
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.6944
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=0.474473
PRIMER_LEFT_4_SEQUENCE=ATCGTGGTAGTGTTGATTGGTG
PRIMER_RIGHT_4_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=363,22
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=60.474
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_02136 Ps_contig_mira-and-tgicl-ass_franssen_19008
SEQUENCE=AAAGGGTGTGGAAAGTGATGATGGCATTAGCATTGATGATAATTGGTGGATGAGTGATGATACATTATATGGGGATAGAAAGAAGGAAAAGAGAAGAAGTAGGAATAATCGTGGTAGTGTTGATTGGTGGTTAGACGGGTTCAGTGGCGAGTTATGGAGAGCGCGTAGGAATAGTTTTGATTCTGCTAGTGGAGACATTCCAAAGAGTGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCCGGGATCGTGGGTATGGAGGAAGTGGTGGAGGAGGCGGTTATTCTCGTGGAGGAGGCGGTGGCGGTGGAAACTGGAGGTGGTTACCATAATGGTGGAGGTGGTTACAA
TARGET=233,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5359
PRIMER_LEFT_PENALTY=0.061436
PRIMER_RIGHT_PENALTY=0.474473
PRIMER_LEFT_SEQUENCE=AGAGCGCGTAGGAATAGTTTTG
PRIMER_RIGHT_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT=157,22
PRIMER_RIGHT=363,22
PRIMER_LEFT_TM=59.939
PRIMER_RIGHT_TM=60.474
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.5991
PRIMER_LEFT_1_PENALTY=0.124655
PRIMER_RIGHT_1_PENALTY=0.474473
PRIMER_LEFT_1_SEQUENCE=TCTGCTAGTGGAGACATTCCAA
PRIMER_RIGHT_1_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT_1=181,22
PRIMER_RIGHT_1=363,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.474
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.6944
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.474473
PRIMER_LEFT_2_SEQUENCE=ATCGTGGTAGTGTTGATTGGTG
PRIMER_RIGHT_2_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT_2=107,22
PRIMER_RIGHT_2=363,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=60.474
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.8048
PRIMER_LEFT_3_PENALTY=0.330336
PRIMER_RIGHT_3_PENALTY=0.474473
PRIMER_LEFT_3_SEQUENCE=GCGCGTAGGAATAGTTTTGATT
PRIMER_RIGHT_3_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=363,22
PRIMER_LEFT_3_TM=59.670
PRIMER_RIGHT_3_TM=60.474
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=1.0964
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=0.474473
PRIMER_LEFT_4_SEQUENCE=TGCTAGTGGAGACATTCCAAAG
PRIMER_RIGHT_4_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT_4=183,22
PRIMER_RIGHT_4=363,22
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=60.474
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_02137 Ps_contig_mira-and-tgicl-ass_franssen_19008
SEQUENCE=AAAGGGTGTGGAAAGTGATGATGGCATTAGCATTGATGATAATTGGTGGATGAGTGATGATACATTATATGGGGATAGAAAGAAGGAAAAGAGAAGAAGTAGGAATAATCGTGGTAGTGTTGATTGGTGGTTAGACGGGTTCAGTGGCGAGTTATGGAGAGCGCGTAGGAATAGTTTTGATTCTGCTAGTGGAGACATTCCAAAGAGTGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCCGGGATCGTGGGTATGGAGGAAGTGGTGGAGGAGGCGGTTATTCTCGTGGAGGAGGCGGTGGCGGTGGAAACTGGAGGTGGTTACCATAATGGTGGAGGTGGTTACAA
TARGET=247,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5359
PRIMER_LEFT_PENALTY=0.061436
PRIMER_RIGHT_PENALTY=0.474473
PRIMER_LEFT_SEQUENCE=AGAGCGCGTAGGAATAGTTTTG
PRIMER_RIGHT_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT=157,22
PRIMER_RIGHT=363,22
PRIMER_LEFT_TM=59.939
PRIMER_RIGHT_TM=60.474
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.5991
PRIMER_LEFT_1_PENALTY=0.124655
PRIMER_RIGHT_1_PENALTY=0.474473
PRIMER_LEFT_1_SEQUENCE=TCTGCTAGTGGAGACATTCCAA
PRIMER_RIGHT_1_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT_1=181,22
PRIMER_RIGHT_1=363,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.474
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.6944
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.474473
PRIMER_LEFT_2_SEQUENCE=ATCGTGGTAGTGTTGATTGGTG
PRIMER_RIGHT_2_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT_2=107,22
PRIMER_RIGHT_2=363,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=60.474
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.8048
PRIMER_LEFT_3_PENALTY=0.330336
PRIMER_RIGHT_3_PENALTY=0.474473
PRIMER_LEFT_3_SEQUENCE=GCGCGTAGGAATAGTTTTGATT
PRIMER_RIGHT_3_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=363,22
PRIMER_LEFT_3_TM=59.670
PRIMER_RIGHT_3_TM=60.474
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=1.0964
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=0.474473
PRIMER_LEFT_4_SEQUENCE=TGCTAGTGGAGACATTCCAAAG
PRIMER_RIGHT_4_SEQUENCE=CCATTATGGTAACCACCTCCAG
PRIMER_LEFT_4=183,22
PRIMER_RIGHT_4=363,22
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=60.474
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_02138 Ps_contig_mira-and-tgicl-ass_franssen_19016
SEQUENCE=AACCTTTATTAACCATAATCATAATCATATTCATGCTTATCTACAGTTTTGAGGACCCCATTATCATTTTGGCTTCTTTATCCATTCCAATTATTCCAAACACACAGGCCCATTCARAACGTGGAATTGAAAGCAAGAGGACAAAAACTTCAACAGGGACCAGTAGACAACAACAACAACAACATCAACAACTCATAACTAATAATTGAGGCCAAACACGCTACAATCACACCGCTACTGGCCCCACCATTGTTACCGGCACCACTGCGGCAAAATGTCAAGGGAGAAATAGGAGGGAGAGGAGAAACATGTTCCAGAGGATGAAGGATGGTATAT
TARGET=167,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02139 Ps_contig_mira-and-tgicl-ass_franssen_19020
SEQUENCE=TAAACCCGGGTACGGGGTTGGGTTGGGGTAGGAGGTGGGTAGGACTGGGTGTGGGTGGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATATTTCAGATGGGAAACTAATGAACAGACTGTTCAGGAGATGCTAAGTTTAGCAACCAAGTACAACAAGGCAGTGCAAGAGGAAGATGAGCTGCCCCCAGAAAAGCTTGCAATTGCTAATGTGGGAAGGCAGGATGCTAAGAAGCATCTTGAAGAGCACGTTTCTAATTTAATGTTCTTCGAACTNTTTGTTCAGTACGTTTGGGAATGATGCTCGACACTAGGTTTGTGTTTCTAAGGTAGKTTAGGACTTCCGTCCATTGTATTCTTCTTCTTCTTNATGATGTCCAG
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02140 Ps_contig_mira-and-tgicl-ass_franssen_19020
SEQUENCE=TAAACCCGGGTACGGGGTTGGGTTGGGGTAGGAGGTGGGTAGGACTGGGTGTGGGTGGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATATTTCAGATGGGAAACTAATGAACAGACTGTTCAGGAGATGCTAAGTTTAGCAACCAAGTACAACAAGGCAGTGCAAGAGGAAGATGAGCTGCCCCCAGAAAAGCTTGCAATTGCTAATGTGGGAAGGCAGGATGCTAAGAAGCATCTTGAAGAGCACGTTTCTAATTTAATGTTCTTCGAACTNTTTGTTCAGTACGTTTGGGAATGATGCTCGACACTAGGTTTGTGTTTCTAAGGTAGKTTAGGACTTCCGTCCATTGTATTCTTCTTCTTCTTNATGATGTCCAG
TARGET=437,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02141 Ps_contig_mira-and-tgicl-ass_franssen_19029
SEQUENCE=GTCGGGGAAGAGTAAAACGTAGAAACTTTTAATACCAGAGTTTTGGGAGAGTGATATGGCACAGTGGTCGTTGCTTATCGTTTTGTTCTGCGTCGCCTCCGCCGCCGCCGGATTCAGCTTCCATGACTCCAATCCTATTCGAATGGTCTCCGACGTGGAGGAGCAGTTCTGAGACACGCNACGAGGGANAGGCAGCA
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1540
PRIMER_LEFT_PENALTY=0.122257
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=TCGGGGAAGAGTAAAACGTAGA
PRIMER_RIGHT_SEQUENCE=ATTGGAGTCATGGAAGCTGAAT
PRIMER_LEFT=1,22
PRIMER_RIGHT=132,22
PRIMER_LEFT_TM=60.122
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.3235
PRIMER_LEFT_1_PENALTY=0.122257
PRIMER_RIGHT_1_PENALTY=0.201288
PRIMER_LEFT_1_SEQUENCE=TCGGGGAAGAGTAAAACGTAGA
PRIMER_RIGHT_1_SEQUENCE=GACCATTCGAATAGGATTGGAG
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=147,22
PRIMER_LEFT_1_TM=60.122
PRIMER_RIGHT_1_TM=59.799
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.3235
PRIMER_LEFT_2_PENALTY=0.122257
PRIMER_RIGHT_2_PENALTY=0.201288
PRIMER_LEFT_2_SEQUENCE=TCGGGGAAGAGTAAAACGTAGA
PRIMER_RIGHT_2_SEQUENCE=CCATTCGAATAGGATTGGAGTC
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=145,22
PRIMER_LEFT_2_TM=60.122
PRIMER_RIGHT_2_TM=59.799
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=0.3701
PRIMER_LEFT_3_PENALTY=0.338329
PRIMER_RIGHT_3_PENALTY=0.031769
PRIMER_LEFT_3_SEQUENCE=CGGGGAAGAGTAAAACGTAGAA
PRIMER_RIGHT_3_SEQUENCE=ATTGGAGTCATGGAAGCTGAAT
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=132,22
PRIMER_LEFT_3_TM=59.662
PRIMER_RIGHT_3_TM=59.968
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=0.5396
PRIMER_LEFT_4_PENALTY=0.338329
PRIMER_RIGHT_4_PENALTY=0.201288
PRIMER_LEFT_4_SEQUENCE=CGGGGAAGAGTAAAACGTAGAA
PRIMER_RIGHT_4_SEQUENCE=GACCATTCGAATAGGATTGGAG
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=147,22
PRIMER_LEFT_4_TM=59.662
PRIMER_RIGHT_4_TM=59.799
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_02142 Ps_contig_mira-and-tgicl-ass_franssen_19030
SEQUENCE=TAGAGTTGAGAAATAACTACACTAAAGAGTTGCAAGATAGATAATACATAACCATAAAAGATTCATGTCTATCTATACCTCTCTTTTTTTTCATCCAACATTATTAATTCATGATATTTATTACTGAGAATGTTGATATTATCAGTTACCGTTGTTGGAAGCACCACCATGTCCACCGTGTCCACCTCCACCATGGTAACCACCACCACCATTATGGTAACCTCCACCGCCATTGTAACCACCTCCACCATTATGGTAACCACCTCCATTGTATCCTCCTCCACCATTGTGGTAACCGCCACCGCCATTGTATCCTCCTCCACCATTGTGGTAACCGCCTCCATTATGGTAACCACCACCACGTCCATAACCTCCATATTTAGCATCGTTTACTTCATCTGTACTTTTCAACAACCTCCTCAGTTAACTTCCCTAGCTGACCACCTCTGATAGAAAATAAGAAGAACCAG
TARGET=199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4467
PRIMER_LEFT_PENALTY=0.412596
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_RIGHT_SEQUENCE=AATATGGAGGTTATGGACGTGG
PRIMER_LEFT=140,22
PRIMER_RIGHT=379,22
PRIMER_LEFT_TM=60.413
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.4871
PRIMER_LEFT_1_PENALTY=0.412596
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_RIGHT_1_SEQUENCE=CAATGGTGGAGGAGGATACAAT
PRIMER_LEFT_1=140,22
PRIMER_RIGHT_1=288,22
PRIMER_LEFT_1_TM=60.413
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.4871
PRIMER_LEFT_2_PENALTY=0.412596
PRIMER_RIGHT_2_PENALTY=0.074466
PRIMER_LEFT_2_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_RIGHT_2_SEQUENCE=CAATGGTGGAGGAGGATACAAT
PRIMER_LEFT_2=140,22
PRIMER_RIGHT_2=327,22
PRIMER_LEFT_2_TM=60.413
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.4871
PRIMER_LEFT_3_PENALTY=0.412596
PRIMER_RIGHT_3_PENALTY=0.074466
PRIMER_LEFT_3_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_RIGHT_3_SEQUENCE=AATGGTGGAGGAGGATACAATG
PRIMER_LEFT_3=140,22
PRIMER_RIGHT_3=287,22
PRIMER_LEFT_3_TM=60.413
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=0.4871
PRIMER_LEFT_4_PENALTY=0.412596
PRIMER_RIGHT_4_PENALTY=0.074466
PRIMER_LEFT_4_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_RIGHT_4_SEQUENCE=AATGGTGGAGGAGGATACAATG
PRIMER_LEFT_4=140,22
PRIMER_RIGHT_4=326,22
PRIMER_LEFT_4_TM=60.413
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_02143 Ps_contig_mira-and-tgicl-ass_franssen_19031
SEQUENCE=TAACAGAGGAAGCAAGGAAAGAGAACTAGAAGACACCTAAAGAAACTAGAGGAAKWCGGTTGAAGAAAGTAGAAGGAAAGGAAGGGCGGGAAGCAGTAGTGGAGGAGCCAATAGCAACTACTGAAAATAGAGTCAGCACCACCACCACCAGCAACTGCAGAGGATGTGATTGAGAACAAAACAGTTGAATCAGTGGAAGCTCCAGCAGCAGAGGAAGTTCCAGTTGAGAATCCTGAAGCTTAAACCAATGGAATGGGTGTGACTGGAACTGGTAAAAAACGGAAGTTTAAATTAATGGTTCTAGATGTTGG
TARGET=137,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02144 Ps_contig_mira-and-tgicl-ass_franssen_19058
SEQUENCE=TTAGCCTCAGCTTCAACAAACACACAGGCCCATTCAGAACGTGGAATTGAAAGCAAGAGGACAAAAACTTCAACAGGGACCAGTAGACAACAACAACAACAWCAWCAACAACTCATAACAATAATTGAGGCCAAACATGTTGCATTCATTAAGCATTGCTCCTGAAGGATCCAAGAGTCTTTTAATTGCTGACTTCCCTTCTTAAGAATGTTAGTTGGTTGGTAGATTGCACGCCACTGCACGACCAACCTAAATTAAAGTCGGTACCCAAACCCAAATTAAACTCCCGTACGTT
TARGET=87,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02145 Ps_contig_mira-and-tgicl-ass_franssen_19070
SEQUENCE=GTCGGGAAATAAATTTATAATAATATAATTCAAAATTCAATTTTATTTAATCSWDAAATACCATGTTTCTACTCAAACACCACACATCAATTAACTCCTAGATAACACACACACAACACAAAAAATAGGGTAAAGATGTTGAGGTTCAAGAACAAAGGTGCTGAATTCTCAGCTAAGGATTACCAAGATCCACCACCAGCACCACTTTTTGATTCAGCTGAAGTTTAAAAAAAAATAGGTCTCTGTACAGAGCTGTTATAGCAGAGTTTGTAGCAACTCTTCTTTTTCTTTA
TARGET=105,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02146 Ps_contig_mira-and-tgicl-ass_franssen_19072
SEQUENCE=ACCACTGACGACGGTAAGGACGTACAAAGAAACCAACCTCCTGCACCGTTCGTTTGTAGCTCTGAATCACTTCATGGTCTTCTGGAGAGCTGGAATAGCCGAGTTCGTTGCAACTTTCTGTTTCTCTACATCACCATCTTGACTATCATGGGTGTCAACAAATCTGATTCCAAGTGTAAAACTGTTGGTATTCAAGGGATTGCTTGGTCTTTTGGTGGCATGATCTTCGCCCTCGTTTACTGCACTGCCGGAATCTCTGGGGGTCACATAAACCCAGCAGTGACATTCGGTTTGTTCTTGGCGAGGAAACTGTCTCTAACAAGAGCAGTTGTTCACATCGTGATGCAGGTTCTTGGTNCTATCTGCGGTGCGTTGGTTGGTTGGTTTAAGGGTTTTAGAAAGG
TARGET=372,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02147 Ps_contig_mira-and-tgicl-ass_franssen_19100
SEQUENCE=GTAAACTGGTGGTGGTGAATAAATCGGTGGAGGAGAAGATTTATATTTGTAAATTGGTGGTGAATAGACWGGWGGAGGAGAYTTATATTTGTAAACTGATGGTGGTGMATARACCGGTGGAGGAGGTGAAGAGTAGACATAGTGTGGTGGAGGTGGTGAGTAAACAGGTGGAGGAGGAGATTTATATTTGTAAACGGGTGGTGGCGAGTAAACAGGTGGAGGAGATTTATATTTGTAAATCGGTGTTGGTGGAGGTGGGAACTTGAATGGCTTTCCAGGTGTTGGAGGAGATATGTGCTTGTAAGGAGGAGGGGGTGAATTGTACTTGTAGACTGGTGGAGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGAAGTGGTAGGACGAGGGGTKGAGGAGTGGGAGTAGGTGTGCACGTGGTGGTGGTGGAGGAGNGTGGTACTTAGTAAGGTCTTCGTTGTGTGGTGTGGTGGAGGAGGAGAGGTGGTAGACGTGGTGTGGTGAGGTGTGTGTACGGTGGT
TARGET=385,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02148 Ps_contig_mira-and-tgicl-ass_franssen_19100
SEQUENCE=GTAAACTGGTGGTGGTGAATAAATCGGTGGAGGAGAAGATTTATATTTGTAAATTGGTGGTGAATAGACWGGWGGAGGAGAYTTATATTTGTAAACTGATGGTGGTGMATARACCGGTGGAGGAGGTGAAGAGTAGACATAGTGTGGTGGAGGTGGTGAGTAAACAGGTGGAGGAGGAGATTTATATTTGTAAACGGGTGGTGGCGAGTAAACAGGTGGAGGAGATTTATATTTGTAAATCGGTGTTGGTGGAGGTGGGAACTTGAATGGCTTTCCAGGTGTTGGAGGAGATATGTGCTTGTAAGGAGGAGGGGGTGAATTGTACTTGTAGACTGGTGGAGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGAAGTGGTAGGACGAGGGGTKGAGGAGTGGGAGTAGGTGTGCACGTGGTGGTGGTGGAGGAGNGTGGTACTTAGTAAGGTCTTCGTTGTGTGGTGTGGTGGAGGAGGAGAGGTGGTAGACGTGGTGTGGTGAGGTGTGTGTACGGTGGT
TARGET=425,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02149 Ps_contig_mira-and-tgicl-ass_franssen_19100
SEQUENCE=GTAAACTGGTGGTGGTGAATAAATCGGTGGAGGAGAAGATTTATATTTGTAAATTGGTGGTGAATAGACWGGWGGAGGAGAYTTATATTTGTAAACTGATGGTGGTGMATARACCGGTGGAGGAGGTGAAGAGTAGACATAGTGTGGTGGAGGTGGTGAGTAAACAGGTGGAGGAGGAGATTTATATTTGTAAACGGGTGGTGGCGAGTAAACAGGTGGAGGAGATTTATATTTGTAAATCGGTGTTGGTGGAGGTGGGAACTTGAATGGCTTTCCAGGTGTTGGAGGAGATATGTGCTTGTAAGGAGGAGGGGGTGAATTGTACTTGTAGACTGGTGGAGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGAAGTGGTAGGACGAGGGGTKGAGGAGTGGGAGTAGGTGTGCACGTGGTGGTGGTGGAGGAGNGTGGTACTTAGTAAGGTCTTCGTTGTGTGGTGTGGTGGAGGAGGAGAGGTGGTAGACGTGGTGTGGTGAGGTGTGTGTACGGTGGT
TARGET=482,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02150 Ps_contig_mira-and-tgicl-ass_franssen_19102
SEQUENCE=TTTTAGTAGTTGGGTTTGGTTCGTCGTAGTCGTCGTCGTCGGTTAAGGGTAAACGAAACTAAACTTTATTTNTAATAACANATGTGGAGATCGTCACTACCATCCCAACCATTGGATTTAATGTTGAAACCGTGGAGTACAAGAACATCAGCTTTCACCGTGTGGGATGTCGGGGGTCAGGACAAGATCCGCCCATTGTGGAGGCACTACTTCCAGAACACTCAGGGTCTTATTTTTGTGGTTGATAGCAATGATAGAGATCGAGTGGTTGAGGCAAGGGATGAGTTGCACAGGATGYTGAAYGAGGACGAACTTAGAGATGCTTGTTTTGTCTTTTGTTTTACGACCCAAACCAAACAAGTACTTCCTTACCCAATGACAATGAATGCTGACAAGAAAC
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02151 Ps_contig_mira-and-tgicl-ass_franssen_19114
SEQUENCE=TCAACGCAGAGTAGTCGGGGACACACAAATCACAACTTCAAATATGGCCTCCTTTTTCCAATCCTTACAAGTGTTGTTCCTTGCTTCCCTTATCCTTGTTCCTCATGGACTTTCTGCTTCTGTTAATAAAATTGTTCCTCATCTTCCTCCTGATCTTGTTCATCATCTTCATCGTATTTTTCATCATTTTGCTCCTCATAATCCATCACCACCTGATAATAAAATTGATCCACTTTATGCATCTTCACCCCGTCCTTACAAACCACCACTGATTCTAACGCCACCATATCATAAGAAAAATTACCNGCAAGATAAAATCCATATACTTAATACAGTACGTCGGTACCCAAACAAACGACTATTAACTTAATTAATTAA
TARGET=367,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_02152 Ps_contig_mira-and-tgicl-ass_franssen_19120
SEQUENCE=CATCAGAAGAAGAAGCTCTAGTCCGTATTGAAGAGAAAACTTCCGCGACTCTGAAAAATGTTTAGGGAAAGGGGAAGAAGAAGGAGTGGTGGTGGAGAGGCGAGGAGGAGGGAGATAAGGCGTGTTGATTTACATAGTAAGAAGGATGGAACATGACACGAGGATTGGCGTAGTTGACGTTTGAATTAGGGTTAGAGAAGTAAAATGAATGGGTCTGAGGTTGAAGAGAAGGAGGAGGAGGGAGGGGAGTGGGTAGGTTGCATAGCCGGGTGGTGGTTGGAGGR
TARGET=230,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02153 Ps_contig_mira-and-tgicl-ass_franssen_19122
SEQUENCE=GATTAAAGGGACGTGGATGGTTTATTTCTTCCTAAAATTAACATGCCGGGATGAGGCTCGTCTTCTTTAAGGCATATGGTGAGCTCCTGATAATACTAGGCTTAATGAAGGTATTGGTGCTGGTCTTGATGAGGAAGATGATGGTGATGCTAATGATTATATCGAGTTTGAGGATGAGGATATTGATAAAAATTTGRAAAAAAAAAAGTTTTTTTTTAGTTTTGTTTGAAGTTAAAAAGTTGAATTCTAATTATTATTACTATCGTTATATGTGATGATGATGAACATGATGATATGTAATGTTTGGGTTTTATGCTGTACTCTATTATGGGATATCACAGTGATGTTGATATCGTGAATTGAATTTCAATGCAACAAGAAATTAAWTWAATARSWYGKTATWAGCTWARWWRAWYTTGTTGTTTCTGTTTTAAGG
TARGET=273,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02154 Ps_contig_mira-and-tgicl-ass_franssen_19147
SEQUENCE=GTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGACTTATATTTWGTAAATCGGTGGTGGAGGAGAAGAATATTTATAAGATTTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACGTGGTGGTGGTGGTGAACTATAATGATAGGTGGAGGAGGTGAGTGCCGACCGGGTGGGTGGGTGGAGTGTTGGTAGTAGTTAGGTTTGGTGGTGGTGGTGGAGAGTAG
TARGET=43,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02155 Ps_contig_mira-and-tgicl-ass_franssen_19147
SEQUENCE=GTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGACTTATATTTWGTAAATCGGTGGTGGAGGAGAAGAATATTTATAAGATTTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACGTGGTGGTGGTGGTGAACTATAATGATAGGTGGAGGAGGTGAGTGCCGACCGGGTGGGTGGGTGGAGTGTTGGTAGTAGTTAGGTTTGGTGGTGGTGGTGGAGAGTAG
TARGET=159,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02156 Ps_contig_mira-and-tgicl-ass_franssen_19147
SEQUENCE=GTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGACTTATATTTWGTAAATCGGTGGTGGAGGAGAAGAATATTTATAAGATTTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACGTGGTGGTGGTGGTGAACTATAATGATAGGTGGAGGAGGTGAGTGCCGACCGGGTGGGTGGGTGGAGTGTTGGTAGTAGTTAGGTTTGGTGGTGGTGGTGGAGAGTAG
TARGET=210,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02157 Ps_contig_mira-and-tgicl-ass_franssen_19147
SEQUENCE=GTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGACTTATATTTWGTAAATCGGTGGTGGAGGAGAAGAATATTTATAAGATTTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACGTGGTGGTGGTGGTGAACTATAATGATAGGTGGAGGAGGTGAGTGCCGACCGGGTGGGTGGGTGGAGTGTTGGTAGTAGTTAGGTTTGGTGGTGGTGGTGGAGAGTAG
TARGET=261,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02158 Ps_contig_mira-and-tgicl-ass_franssen_19147
SEQUENCE=GTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGACTTATATTTWGTAAATCGGTGGTGGAGGAGAAGAATATTTATAAGATTTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACGTGGTGGTGGTGGTGAACTATAATGATAGGTGGAGGAGGTGAGTGCCGACCGGGTGGGTGGGTGGAGTGTTGGTAGTAGTTAGGTTTGGTGGTGGTGGTGGAGAGTAG
TARGET=296,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02159 Ps_contig_mira-and-tgicl-ass_franssen_19199
SEQUENCE=CAACGCAGAGTACGCGGGGATCTTTAAAAATTATCACTCTTAACTAGTCACATTACATCCTTTTCTTTTATGATGATGACCGTCATGATTTCAATAATACCAATCATTTTGATTCACACTTTATTTTATGTTGGATTTTCTGATGACATGCCACTTACCACTACACTTCCACAATCACCAACATCACCTCTCCCACATTTTCACTTGTACMATCAAAATTCCAATTCATATCCTTCATCTACACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCTAGGAGAAGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTTCATCAGTTTCTACCAACTCTTTATTACGANCTA
TARGET=292,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02160 Ps_contig_mira-and-tgicl-ass_franssen_19201
SEQUENCE=CTTCAGTGGTTCCATTGCATTTCCAGTTACCGCCGACGAAGAACTTTCTGCCCATGTTTTGGTTAATGTTTTCGATTTAAACGAGGAAGTGAAAAACCCTTCTTCTTGTTGTTGTTGTTCTSAGTGTTTGTTGTTCACCCTTTATATTATAGATAAACTGAAGAATATGCTTAAC
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02161 Ps_contig_mira-and-tgicl-ass_franssen_19207
SEQUENCE=TGACACTATGGTCATGGTGGTGGTAGTTACCATGAATGGTGGCGGCAGTTACCACGGTGGACATGGTGGACATGGAGGACATGGTGGACATGGTGGTRGTTTCCAACAATGAGGTTGTTGAAACTTCAAATGACGTAAATGATGCCAAATATGGACACTACGGTCATGGTGGTGGTAGTTACCATGGTGGTGGTGGCGGTTACCATGGTGGACATGGAGGACATGGAGGACACGGTGGACACGGTGGTGCTTCCGACAATGGCAACTGATTATATCATGATGCATGATGCACATTCAAAGTAAATTAAGTTTCATGAATAGAATAGTTGTTTGGTTAGTAAAAATAAAAGAGGTACAAATAGTCTATCATTATGTTGTGTTATTA
TARGET=185,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02162 Ps_contig_mira-and-tgicl-ass_franssen_19215
SEQUENCE=CTATATCAAGAGATAACAAATGCCTCTCATATAACAAAGAACATACAGCAAAAACAAAACAAATGATGAGCAAGGAAGATCAACATGACTTGCTACTAATACAGCTCTATTCGGTTCCGGCAATTTTTCTTTTTCAGTGCCTCAATATCCGTAGYTAGTTCCTTTCTACTAGACTTAAACAGAAGGTACCGGTAGRCAAACCATCCAGTGTACCCTAGTCCTACCAACTCCATAATCTTTGGAAGCAAGGGGACTGAGTTGATGGCACCTACAAGAATTGAAGATAACCAAACAGCAACCAAACCACCACCACCATACAGAAGCACTGTGGACTTGTTTTCAAGACCATCCCACTTCTCCTTCAAATCGGTAAACAACTCATTGGTATCGAGAGAAGTAGATGTATCATCAGAAGAAGAAGCTCTAGTCCGATGAAGAAGAAACTTCCGCGACTCTAGAAAAATTGGTTTAAAGGGGGAAAARGGGGAAAGAAAGGAAAGGGAGGTTGGGTTGGGTRGGAGGAGGGCGGAAGGGAGGGAGGGAGAAGTAAGGACAAAGCAGGAGTAGGCGG
TARGET=303,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02163 Ps_contig_mira-and-tgicl-ass_franssen_19215
SEQUENCE=CTATATCAAGAGATAACAAATGCCTCTCATATAACAAAGAACATACAGCAAAAACAAAACAAATGATGAGCAAGGAAGATCAACATGACTTGCTACTAATACAGCTCTATTCGGTTCCGGCAATTTTTCTTTTTCAGTGCCTCAATATCCGTAGYTAGTTCCTTTCTACTAGACTTAAACAGAAGGTACCGGTAGRCAAACCATCCAGTGTACCCTAGTCCTACCAACTCCATAATCTTTGGAAGCAAGGGGACTGAGTTGATGGCACCTACAAGAATTGAAGATAACCAAACAGCAACCAAACCACCACCACCATACAGAAGCACTGTGGACTTGTTTTCAAGACCATCCCACTTCTCCTTCAAATCGGTAAACAACTCATTGGTATCGAGAGAAGTAGATGTATCATCAGAAGAAGAAGCTCTAGTCCGATGAAGAAGAAACTTCCGCGACTCTAGAAAAATTGGTTTAAAGGGGGAAAARGGGGAAAGAAAGGAAAGGGAGGTTGGGTTGGGTRGGAGGAGGGCGGAAGGGAGGGAGGGAGAAGTAAGGACAAAGCAGGAGTAGGCGG
TARGET=531,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02164 Ps_contig_mira-and-tgicl-ass_franssen_19219
SEQUENCE=ACCAGAAGTGTACGCCATGCTTGACGGTTAGCCTCGGTGTTCTCTAGCCCAATTGAATCCAACCGCTTTCCACATGTAGCATTGGACTCATCCATGGCCAAAATACCACGCCCTGGTGAAGCAATTGTTTTCGCAGTTTTGACGAGCTCGTCGGCATAGGAACCAGCTCGGATGGTAAAACTTGATGAATTAGCAGGGTTGCATCTGGCAACAGACATAGAAGAAGGTTGACGGAGGAGAGTTTTTCCCTTCACCCATTCAGACTTATCAAGAACAAGTGATGATGATTTGAAAATAGATGCTGAAGCAGAAGCCATAGTTTCACAAACTCACTCACTCACTGCAATACTTAATAACCCCAAACAAAACAAAACAAAACCTAACTTTAAACCAAACCCCCG
TARGET=328,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0246
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.017652
PRIMER_LEFT_SEQUENCE=ATGAATTAGCAGGGTTGCATCT
PRIMER_RIGHT_SEQUENCE=GGGGGTTTGGTTTAAAGTTAGG
PRIMER_LEFT=184,22
PRIMER_RIGHT=399,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.982
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=0.0314
PRIMER_LEFT_1_PENALTY=0.013714
PRIMER_RIGHT_1_PENALTY=0.017652
PRIMER_LEFT_1_SEQUENCE=AATAGATGCTGAAGCAGAAGCC
PRIMER_RIGHT_1_SEQUENCE=GGGGGTTTGGTTTAAAGTTAGG
PRIMER_LEFT_1=293,22
PRIMER_RIGHT_1=399,22
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=59.982
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=107
PRIMER_PAIR_PENALTY_2=0.1383
PRIMER_LEFT_2_PENALTY=0.120688
PRIMER_RIGHT_2_PENALTY=0.017652
PRIMER_LEFT_2_SEQUENCE=TAGAAGAAGGTTGACGGAGGAG
PRIMER_RIGHT_2_SEQUENCE=GGGGGTTTGGTTTAAAGTTAGG
PRIMER_LEFT_2=217,22
PRIMER_RIGHT_2=399,22
PRIMER_LEFT_2_TM=59.879
PRIMER_RIGHT_2_TM=59.982
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=0.1409
PRIMER_LEFT_3_PENALTY=0.123278
PRIMER_RIGHT_3_PENALTY=0.017652
PRIMER_LEFT_3_SEQUENCE=CATAGAAGAAGGTTGACGGAGG
PRIMER_RIGHT_3_SEQUENCE=GGGGGTTTGGTTTAAAGTTAGG
PRIMER_LEFT_3=215,22
PRIMER_RIGHT_3=399,22
PRIMER_LEFT_3_TM=60.123
PRIMER_RIGHT_3_TM=59.982
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=0.2328
PRIMER_LEFT_4_PENALTY=0.215198
PRIMER_RIGHT_4_PENALTY=0.017652
PRIMER_LEFT_4_SEQUENCE=AGAGTTTTTCCCTTCACCCATT
PRIMER_RIGHT_4_SEQUENCE=GGGGGTTTGGTTTAAAGTTAGG
PRIMER_LEFT_4=237,22
PRIMER_RIGHT_4=399,22
PRIMER_LEFT_4_TM=60.215
PRIMER_RIGHT_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_02165 Ps_contig_mira-and-tgicl-ass_franssen_19219
SEQUENCE=ACCAGAAGTGTACGCCATGCTTGACGGTTAGCCTCGGTGTTCTCTAGCCCAATTGAATCCAACCGCTTTCCACATGTAGCATTGGACTCATCCATGGCCAAAATACCACGCCCTGGTGAAGCAATTGTTTTCGCAGTTTTGACGAGCTCGTCGGCATAGGAACCAGCTCGGATGGTAAAACTTGATGAATTAGCAGGGTTGCATCTGGCAACAGACATAGAAGAAGGTTGACGGAGGAGAGTTTTTCCCTTCACCCATTCAGACTTATCAAGAACAAGTGATGATGATTTGAAAATAGATGCTGAAGCAGAAGCCATAGTTTCACAAACTCACTCACTCACTGCAATACTTAATAACCCCAAACAAAACAAAACAAAACCTAACTTTAAACCAAACCCCCG
TARGET=361,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0246
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.017652
PRIMER_LEFT_SEQUENCE=ATGAATTAGCAGGGTTGCATCT
PRIMER_RIGHT_SEQUENCE=GGGGGTTTGGTTTAAAGTTAGG
PRIMER_LEFT=184,22
PRIMER_RIGHT=399,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.982
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=0.0314
PRIMER_LEFT_1_PENALTY=0.013714
PRIMER_RIGHT_1_PENALTY=0.017652
PRIMER_LEFT_1_SEQUENCE=AATAGATGCTGAAGCAGAAGCC
PRIMER_RIGHT_1_SEQUENCE=GGGGGTTTGGTTTAAAGTTAGG
PRIMER_LEFT_1=293,22
PRIMER_RIGHT_1=399,22
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=59.982
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=107
PRIMER_PAIR_PENALTY_2=0.1383
PRIMER_LEFT_2_PENALTY=0.120688
PRIMER_RIGHT_2_PENALTY=0.017652
PRIMER_LEFT_2_SEQUENCE=TAGAAGAAGGTTGACGGAGGAG
PRIMER_RIGHT_2_SEQUENCE=GGGGGTTTGGTTTAAAGTTAGG
PRIMER_LEFT_2=217,22
PRIMER_RIGHT_2=399,22
PRIMER_LEFT_2_TM=59.879
PRIMER_RIGHT_2_TM=59.982
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=0.1409
PRIMER_LEFT_3_PENALTY=0.123278
PRIMER_RIGHT_3_PENALTY=0.017652
PRIMER_LEFT_3_SEQUENCE=CATAGAAGAAGGTTGACGGAGG
PRIMER_RIGHT_3_SEQUENCE=GGGGGTTTGGTTTAAAGTTAGG
PRIMER_LEFT_3=215,22
PRIMER_RIGHT_3=399,22
PRIMER_LEFT_3_TM=60.123
PRIMER_RIGHT_3_TM=59.982
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=0.2328
PRIMER_LEFT_4_PENALTY=0.215198
PRIMER_RIGHT_4_PENALTY=0.017652
PRIMER_LEFT_4_SEQUENCE=AGAGTTTTTCCCTTCACCCATT
PRIMER_RIGHT_4_SEQUENCE=GGGGGTTTGGTTTAAAGTTAGG
PRIMER_LEFT_4=237,22
PRIMER_RIGHT_4=399,22
PRIMER_LEFT_4_TM=60.215
PRIMER_RIGHT_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_02166 Ps_contig_mira-and-tgicl-ass_franssen_19222
SEQUENCE=GTCGGGGGCGGTGCAGTGCAGCCTTTTTCTCTGCCCTCCCAAAAATTGAAGAAAAAAACCAAAAAATTTCAGTTTCTCCGCCGTTACGATCACCGGAGAGTTTATCCCTACTCCATTAACACTCTCAAAGAAACAAATCCAAAAAATTGAAACTGCTAAGAGTGGTTAGGTTTAACTTATTATTATTACCGTTGATTTGTGTAAGTTTTGATGGCGCAGATTCAGGTTCAGCAGGTCCGTTCCGGCACCTTCGGAACGGCGTCATYSYMGTTTCGGCCCCGGTACCCG
TARGET=177,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02167 Ps_contig_mira-and-tgicl-ass_franssen_19233
SEQUENCE=TGACCACCACCATAGTGTCCACTTCCATAGTGTCCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATNTTGGTTTCGGGGCTGTCTTTTTATTTTTTATTTGTTGTTTTTGATGTTATTAGCCACAGTCG
TARGET=82,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8058
PRIMER_LEFT_PENALTY=0.669753
PRIMER_RIGHT_PENALTY=0.136017
PRIMER_LEFT_SEQUENCE=GTCCACTTCCATAGTGTCCACC
PRIMER_RIGHT_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT=16,22
PRIMER_RIGHT=253,22
PRIMER_LEFT_TM=60.670
PRIMER_RIGHT_TM=59.864
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.8058
PRIMER_LEFT_1_PENALTY=0.669753
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=GTCCACTTCCATAGTGTCCACC
PRIMER_RIGHT_1_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=254,22
PRIMER_LEFT_1_TM=60.670
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.8058
PRIMER_LEFT_2_PENALTY=0.669753
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=GTCCACTTCCATAGTGTCCACC
PRIMER_RIGHT_2_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=252,22
PRIMER_LEFT_2_TM=60.670
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=1.0964
PRIMER_LEFT_3_PENALTY=0.669753
PRIMER_RIGHT_3_PENALTY=0.426682
PRIMER_LEFT_3_SEQUENCE=GTCCACTTCCATAGTGTCCACC
PRIMER_RIGHT_3_SEQUENCE=ACATGAGGAGGGTAGGTGTGAG
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=166,22
PRIMER_LEFT_3_TM=60.670
PRIMER_RIGHT_3_TM=60.427
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=1.1646
PRIMER_LEFT_4_PENALTY=1.098207
PRIMER_RIGHT_4_PENALTY=0.066394
PRIMER_LEFT_4_SEQUENCE=ACCCTCACCCTGTCTACCACT
PRIMER_RIGHT_4_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT_4=58,21
PRIMER_RIGHT_4=326,22
PRIMER_LEFT_4_TM=59.902
PRIMER_RIGHT_4_TM=59.934
PRIMER_LEFT_4_GC_PERCENT=57.143
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_02168 Ps_contig_mira-and-tgicl-ass_franssen_19233
SEQUENCE=TGACCACCACCATAGTGTCCACTTCCATAGTGTCCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATNTTGGTTTCGGGGCTGTCTTTTTATTTTTTATTTGTTGTTTTTGATGTTATTAGCCACAGTCG
TARGET=128,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5407
PRIMER_LEFT_PENALTY=0.474295
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT=96,22
PRIMER_RIGHT=326,22
PRIMER_LEFT_TM=60.474
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.6103
PRIMER_LEFT_1_PENALTY=0.474295
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_1_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=254,22
PRIMER_LEFT_1_TM=60.474
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.6103
PRIMER_LEFT_2_PENALTY=0.474295
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_2_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT_2=96,22
PRIMER_RIGHT_2=253,22
PRIMER_LEFT_2_TM=60.474
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=158
PRIMER_PAIR_PENALTY_3=0.6379
PRIMER_LEFT_3_PENALTY=0.474295
PRIMER_RIGHT_3_PENALTY=0.163604
PRIMER_LEFT_3_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_3_SEQUENCE=TATATGTGAAGAATCCCCTCCG
PRIMER_LEFT_3=96,22
PRIMER_RIGHT_3=363,22
PRIMER_LEFT_3_TM=60.474
PRIMER_RIGHT_3_TM=60.164
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.7976
PRIMER_LEFT_4_PENALTY=0.474295
PRIMER_RIGHT_4_PENALTY=0.323355
PRIMER_LEFT_4_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_4_SEQUENCE=TCTTTGCTTTCACACTTTCTGC
PRIMER_LEFT_4=96,22
PRIMER_RIGHT_4=321,22
PRIMER_LEFT_4_TM=60.474
PRIMER_RIGHT_4_TM=59.677
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_02169 Ps_contig_mira-and-tgicl-ass_franssen_19233
SEQUENCE=TGACCACCACCATAGTGTCCACTTCCATAGTGTCCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATNTTGGTTTCGGGGCTGTCTTTTTATTTTTTATTTGTTGTTTTTGATGTTATTAGCCACAGTCG
TARGET=188,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3531
PRIMER_LEFT_PENALTY=0.286660
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=GCCATACAAGTATCCATCACCA
PRIMER_RIGHT_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT=110,22
PRIMER_RIGHT=326,22
PRIMER_LEFT_TM=59.713
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.4350
PRIMER_LEFT_1_PENALTY=0.368624
PRIMER_RIGHT_1_PENALTY=0.066394
PRIMER_LEFT_1_SEQUENCE=ATGTTCCTACCCCAGTTTACCA
PRIMER_RIGHT_1_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT_1=163,22
PRIMER_RIGHT_1=326,22
PRIMER_LEFT_1_TM=59.631
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.4350
PRIMER_LEFT_2_PENALTY=0.368624
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=TGTTCCTACCCCAGTTTACCAT
PRIMER_RIGHT_2_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT_2=164,22
PRIMER_RIGHT_2=326,22
PRIMER_LEFT_2_TM=59.631
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=0.4350
PRIMER_LEFT_3_PENALTY=0.368624
PRIMER_RIGHT_3_PENALTY=0.066394
PRIMER_LEFT_3_SEQUENCE=CATGTTCCTACCCCAGTTTACC
PRIMER_RIGHT_3_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT_3=162,22
PRIMER_RIGHT_3=326,22
PRIMER_LEFT_3_TM=59.631
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.4503
PRIMER_LEFT_4_PENALTY=0.286660
PRIMER_RIGHT_4_PENALTY=0.163604
PRIMER_LEFT_4_SEQUENCE=GCCATACAAGTATCCATCACCA
PRIMER_RIGHT_4_SEQUENCE=TATATGTGAAGAATCCCCTCCG
PRIMER_LEFT_4=110,22
PRIMER_RIGHT_4=363,22
PRIMER_LEFT_4_TM=59.713
PRIMER_RIGHT_4_TM=60.164
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_02170 Ps_contig_mira-and-tgicl-ass_franssen_19240
SEQUENCE=GTGTCTGCGCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCGGACCTTTGCCGGAGAATGGTCTCGGCAAGCTCCGAAAACACCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGGTACGCATGAACTTTAACCATTAACCGTCGTTGGATTTTCCCAAATTATAGCACTTCAAAAAGTATACATAATAATATAAATTTATAGAATGTGTCCATGCTACTTAAATAACGAGAATTATACTGTACAAAATATATAATGAGAATTTTACTTATTAATTTTTTTTGTCTAAAGTATTATTTACCGTTGGCGCTGGTGAGTTTTGATCAGAATCAGAGGGTAGGRAACG
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02171 Ps_contig_mira-and-tgicl-ass_franssen_19247
SEQUENCE=TAGTTACGTCGGGGACCGATTGATAAGAATACAATTATCAAAAAGACATAACAAATCTTTTCAATTTCATTGCAATATACACACACGATGGCCGCATCATCATCATCATCCATGGCTCTCTCTTCTCCAACCTTGGCTGGCAAGCAACTCAAGCTGACCCCATCAAGCCAAGAATTGGGAGCTTGCAAGGTTCACCATGAGGAAGTCTGCTACCACG
TARGET=95,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6934
PRIMER_LEFT_PENALTY=0.627348
PRIMER_RIGHT_PENALTY=1.066065
PRIMER_LEFT_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_SEQUENCE=TGGTAGCAGACTTCCTCATGGT
PRIMER_LEFT=69,22
PRIMER_RIGHT=214,22
PRIMER_LEFT_TM=59.373
PRIMER_RIGHT_TM=61.066
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=1.6934
PRIMER_LEFT_1_PENALTY=0.627348
PRIMER_RIGHT_1_PENALTY=1.066065
PRIMER_LEFT_1_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_1_SEQUENCE=GTGGTAGCAGACTTCCTCATGG
PRIMER_LEFT_1=69,22
PRIMER_RIGHT_1=215,22
PRIMER_LEFT_1_TM=59.373
PRIMER_RIGHT_1_TM=61.066
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=1.8890
PRIMER_LEFT_2_PENALTY=0.627348
PRIMER_RIGHT_2_PENALTY=1.261614
PRIMER_LEFT_2_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_2_SEQUENCE=TGGTAGCAGACTTCCTCATGG
PRIMER_LEFT_2=69,22
PRIMER_RIGHT_2=214,21
PRIMER_LEFT_2_TM=59.373
PRIMER_RIGHT_2_TM=60.262
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=2.0399
PRIMER_LEFT_3_PENALTY=1.286253
PRIMER_RIGHT_3_PENALTY=0.753664
PRIMER_LEFT_3_SEQUENCE=GGGGACCGATTGATAAGAATAC
PRIMER_RIGHT_3_SEQUENCE=AGGTTGGAGAAGAGAGAGCCAT
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=132,22
PRIMER_LEFT_3_TM=58.714
PRIMER_RIGHT_3_TM=60.754
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=123
PRIMER_PAIR_PENALTY_4=2.2604
PRIMER_LEFT_4_PENALTY=0.627348
PRIMER_RIGHT_4_PENALTY=1.633005
PRIMER_LEFT_4_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_4_SEQUENCE=CGTGGTAGCAGACTTCCTCAT
PRIMER_LEFT_4=69,22
PRIMER_RIGHT_4=216,21
PRIMER_LEFT_4_TM=59.373
PRIMER_RIGHT_4_TM=59.367
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_02172 Ps_contig_mira-and-tgicl-ass_franssen_19249
SEQUENCE=CGTCCTCGTCGTTCCACGCAGCMACACCACCCTCGCAGCTGCTCCCACCTCCCAGCCACYYCCACGCACCCGCCCTCCTTCTTCCCACGCTTCCTCCCCTCCCACGCTCCAACACCAACCGTGGACCTGCCTCTTCTGCCTCTCCTCCCGCACCTACTCCAACCTCCCCCAGAAAGTGGTCCCGCCTCTTCACCAACCGATTCTCCACCTTCTCCCTCGGCAGCATTTTCCTTCAGCAAACCCATTGTTGCTGCAACTGCTCTCTCTGCTGCTTTCTTTGCCATCGTTTTCTAGGACTCGTCCACGTGGATCTTGATCTGACTTTTCATTGACGGACGGACGGGATCTGTTTGATTTAAATTCTGAGTAGTCGTTTTTATTTTGTTTCATGTGTCTTATTTATTACTTTTTCGTCATGTTGTTTGTTGATTCGGACGGACGGGATCGATCTTCATGATTATGATGATGTATTTTAGTTTGWGTAATTCTATTTTGCTTTGGTNTTCCACACGTTGTA
TARGET=331,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02173 Ps_contig_mira-and-tgicl-ass_franssen_19250
SEQUENCE=GGTGAGAGGAAATAAATAATATGCTGGCGTTAATTATASGGACGTGAGTGGGGAAATTGTTCTGATCCCACGGCCCATATTTCCTATGCTTTTATAACTACGAGCTTCGAAAGTAACGCTCATCTTTCTTCATATCTCTCTCACTTTCACTCCCAACCAAAACACACACACATCATGGGTTTGCAGGAGGATTTTGAGGAGCATGCTGCGAAAGTTAAGACTCTAACAGAGAGTCCATCAAATGAAAACTTTCTTCATCCTTTAKTRKGATTATTTGTTCCATAATCCATAACAACGCGA
TARGET=162,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02174 Ps_contig_mira-and-tgicl-ass_franssen_19254
SEQUENCE=ACGAACCCGTCCGGGGTAAACCCCGAAAAACGGTTTAAGGTAAAGGAAGGGAAAAGGGGTTGGTAAAAAGGTAAAAACGAAACAACCCAACGTACKTCCTTTCGGTAGGAAAACGTTTACTACCGACTCTGTACAGACTCATCAAGACTCCTCAAGTGAAGAGGAAGGAGAAGATGGTGAGAAAAGAAAGAAGAAGAAGGAAAAGAAGAAGAAAGAAGACACTTCGGTGCCAGTTGAGAAAGTTGAGGTTGTTGAAGGAACAACCGGTACTGAGGAAAAGAAGGGTTTCCTTGACAAAATTAAGGAGAAGCTTCCCGGAGGACACAAGAAAACAGAGGAAGTAACAACTCCTCCACCAGTTGTCGCTGCTCATGTTCCAACTGAGACAACAGCTACAACAACAACTATTCATGAGGGAGAGAAGAAAGGTATTTTGGAAAAGATCAAAGAGAAGCTTCCTGGTTATCATGCTAAGACTGCTACTGATGGTGAAGACAAAG
TARGET=188,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02175 Ps_contig_mira-and-tgicl-ass_franssen_19257
SEQUENCE=TTAAAACTTTAACGAAACTAAATTAGTATCTAGAACTAAGTTCCGTGAGCATACCATGATAACCATATTGAATGTTTGCATCTTCTTGATGATTACCTTTTTTAAAATAACCGGCCCGGCCTAATTTTCATTGAGTCTTGTCTTTATAGGAACAAAATACGTTCACATTTATGCCATCTTTGTTGCAATCTCGTATCTCAACACCTTTCTTTACTTGGCCATAACCCATGGATAGTAAATTTTTGTGGCAATGGAGAAACACAATCATTATTTTTAAGCTTGCAGTATGTTGGTGGCCATGTCTGAGACAGTTTAAAATGGTCGAATAAAAGAGGTGGTGGTGGTGGTGGTGGACGAGGAGGTGGTGGAGATCTTATCCTAGGTGGTGGTGGTGGAGGAGAACTTCTCCTAGGAGGTGGTGGTGGAGATCTTCTCGGTGGTGGTGGTGGAGATCTTCTCCTAGGTGGTGGAGATCTTCTCTTACTTTCCTCTAGAGGTGTAGATGAAGGATATGAATTGGAATTTTGATTGTACAAGTGAAAAKGTGGGAGAGGTGATGTTGGTGATTGTGGAAGTGTAGTGGTAAGTGGCATGTCATCAGAAAATCCAACATAAAATAAAGTGTGAATACAAAATGATTGGTTATTATTGAAATCATGACGGTCATNATAAATAAAAGAAAAGGA
TARGET=334,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02176 Ps_contig_mira-and-tgicl-ass_franssen_19257
SEQUENCE=TTAAAACTTTAACGAAACTAAATTAGTATCTAGAACTAAGTTCCGTGAGCATACCATGATAACCATATTGAATGTTTGCATCTTCTTGATGATTACCTTTTTTAAAATAACCGGCCCGGCCTAATTTTCATTGAGTCTTGTCTTTATAGGAACAAAATACGTTCACATTTATGCCATCTTTGTTGCAATCTCGTATCTCAACACCTTTCTTTACTTGGCCATAACCCATGGATAGTAAATTTTTGTGGCAATGGAGAAACACAATCATTATTTTTAAGCTTGCAGTATGTTGGTGGCCATGTCTGAGACAGTTTAAAATGGTCGAATAAAAGAGGTGGTGGTGGTGGTGGTGGACGAGGAGGTGGTGGAGATCTTATCCTAGGTGGTGGTGGTGGAGGAGAACTTCTCCTAGGAGGTGGTGGTGGAGATCTTCTCGGTGGTGGTGGTGGAGATCTTCTCCTAGGTGGTGGAGATCTTCTCTTACTTTCCTCTAGAGGTGTAGATGAAGGATATGAATTGGAATTTTGATTGTACAAGTGAAAAKGTGGGAGAGGTGATGTTGGTGATTGTGGAAGTGTAGTGGTAAGTGGCATGTCATCAGAAAATCCAACATAAAATAAAGTGTGAATACAAAATGATTGGTTATTATTGAAATCATGACGGTCATNATAAATAAAAGAAAAGGA
TARGET=382,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02177 Ps_contig_mira-and-tgicl-ass_franssen_19257
SEQUENCE=TTAAAACTTTAACGAAACTAAATTAGTATCTAGAACTAAGTTCCGTGAGCATACCATGATAACCATATTGAATGTTTGCATCTTCTTGATGATTACCTTTTTTAAAATAACCGGCCCGGCCTAATTTTCATTGAGTCTTGTCTTTATAGGAACAAAATACGTTCACATTTATGCCATCTTTGTTGCAATCTCGTATCTCAACACCTTTCTTTACTTGGCCATAACCCATGGATAGTAAATTTTTGTGGCAATGGAGAAACACAATCATTATTTTTAAGCTTGCAGTATGTTGGTGGCCATGTCTGAGACAGTTTAAAATGGTCGAATAAAAGAGGTGGTGGTGGTGGTGGTGGACGAGGAGGTGGTGGAGATCTTATCCTAGGTGGTGGTGGTGGAGGAGAACTTCTCCTAGGAGGTGGTGGTGGAGATCTTCTCGGTGGTGGTGGTGGAGATCTTCTCCTAGGTGGTGGAGATCTTCTCTTACTTTCCTCTAGAGGTGTAGATGAAGGATATGAATTGGAATTTTGATTGTACAAGTGAAAAKGTGGGAGAGGTGATGTTGGTGATTGTGGAAGTGTAGTGGTAAGTGGCATGTCATCAGAAAATCCAACATAAAATAAAGTGTGAATACAAAATGATTGGTTATTATTGAAATCATGACGGTCATNATAAATAAAAGAAAAGGA
TARGET=436,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02178 Ps_contig_mira-and-tgicl-ass_franssen_19261
SEQUENCE=TAATATTATTATTACTCCGCCTGCTCAAATACGTGTCACGATATTCTTTATTAGTTATATCTGCAAACTCGTTCAACCCAACTCTATAACTGTGATTTTGAGCATTATGCTCATCTATGAATATTAAGTTATCCTTAAAAATTTGAAATCTTTGGTCTTTCTCTCCTAACCCGTTATACACTTTTTGGTGTTTTACCAACCACTCCTCGTACATGTCATAACCTCCTCGTTATTACGACCATTTGGTATGTTTGATGCTAATGAGAAAGTGATAAAGAAGAAGAAGAGAAAGAAGGGTAGTATGGTCATTGATGTCATGATTGTGTTTGGCTAAGAAGAGAAAGGATGGGTTAAAGGTTTTGGTGTCCCCGNGTACCTNGTGTANAC
TARGET=275,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1247
PRIMER_LEFT_PENALTY=0.109754
PRIMER_RIGHT_PENALTY=0.014945
PRIMER_LEFT_SEQUENCE=CGACCATTTGGTATGTTTGATG
PRIMER_RIGHT_SEQUENCE=ACCAAAACCTTTAACCCATCCT
PRIMER_LEFT=235,22
PRIMER_RIGHT=363,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.985
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=129
PRIMER_PAIR_PENALTY_1=0.2419
PRIMER_LEFT_1_PENALTY=0.226971
PRIMER_RIGHT_1_PENALTY=0.014945
PRIMER_LEFT_1_SEQUENCE=GGTGTTTTACCAACCACTCCTC
PRIMER_RIGHT_1_SEQUENCE=ACCAAAACCTTTAACCCATCCT
PRIMER_LEFT_1=186,22
PRIMER_RIGHT_1=363,22
PRIMER_LEFT_1_TM=59.773
PRIMER_RIGHT_1_TM=59.985
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.2593
PRIMER_LEFT_2_PENALTY=0.109754
PRIMER_RIGHT_2_PENALTY=0.149516
PRIMER_LEFT_2_SEQUENCE=CGACCATTTGGTATGTTTGATG
PRIMER_RIGHT_2_SEQUENCE=CCATCCTTTCTCTTCTTAGCCA
PRIMER_LEFT_2=235,22
PRIMER_RIGHT_2=348,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.850
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=0.3613
PRIMER_LEFT_3_PENALTY=0.226971
PRIMER_RIGHT_3_PENALTY=0.134318
PRIMER_LEFT_3_SEQUENCE=GGTGTTTTACCAACCACTCCTC
PRIMER_RIGHT_3_SEQUENCE=AGCCAAACACAATCATGACATC
PRIMER_LEFT_3=186,22
PRIMER_RIGHT_3=331,22
PRIMER_LEFT_3_TM=59.773
PRIMER_RIGHT_3_TM=59.866
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.3765
PRIMER_LEFT_4_PENALTY=0.226971
PRIMER_RIGHT_4_PENALTY=0.149516
PRIMER_LEFT_4_SEQUENCE=GGTGTTTTACCAACCACTCCTC
PRIMER_RIGHT_4_SEQUENCE=CCATCCTTTCTCTTCTTAGCCA
PRIMER_LEFT_4=186,22
PRIMER_RIGHT_4=348,22
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=59.850
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_02179 Ps_contig_mira-and-tgicl-ass_franssen_19276
SEQUENCE=AGTACCGGCTCGTTCYTTCTCACACCTCTACTTCTCACTGGCACCGTAGTGTTCTTCTTTCTTCTTTTTCCTTCTTCTTCTTTCTTTTCTTCACCATCTTCTCCTTCCTCTTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGGTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCACCTTGTGACCAAACTCGTGGGCAATCGCCTCATCTTGAGCAGGCTTATGTTCTTCATCCTTTTTCTTACCACCGAGAAAATCAAAAACACCTCTGTCCTTGATCTCTGTCTCAGAGTTGGTTGCGGAGGTAGTCTCCTCGTACTTGTTCTGATTCTCCTCAGCCATGATGATCACTACTAACAAGATGGATCGAACTAAGGAAATTAATTAACTGWAAATGGATTATGTAGCTGTAACTCTGAAATGAAAGGTAAAG
TARGET=71,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02180 Ps_contig_mira-and-tgicl-ass_franssen_19278
SEQUENCE=TTCGGGGGTCTTTCTCTGCAAAACTTCAAGAACGTCGAGGTTAGAGTTCTTGTGACAAATGAATTATCAGCAAGGGGTTTGGATGTAGCAGAATGTGATCTTGTGGTTAACCTCGAATTGCCGACAGATTCAATTCACTATGCACATCGAGCCGGTCGAACCGGTCGGCTTGGTAGGAACGGTACAGTGCTAACCATATGTGAAGAGTCAGAGGTTTTTGTTGTGAGAAAATTGAAGAAGCAACTTGAATTACCGATAGCATGCTGTAATTTTGTCGAAGGAAAGCTTCTTGTGACAGAAGAAGAAGAGCATACACTAACCGACACTTCTTGAAGACGAGGTGTTGTAATTTTGTAACTTGTTTAGATGACATTGTTTCCATTTTTGTGTTTCATTCTTCATTGAAGTTTCTTTCACTCTCTGCTAATGTTTATTTATATGCATCAAAGCTACATTTAGAGTATTGTTTTCTTTCTTTGTCTTATTTGTTTCTAGTTTTTTAATTATTTGTCTCTCATATCGCGTACATAAACCAAATTTCAAAAGATCCATTTTTGAAGAACTAAAAACTGTTGCTGCATATTAATTCCATGCCATTTCTCCTAAGT
TARGET=297,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8293
PRIMER_LEFT_PENALTY=0.249074
PRIMER_RIGHT_PENALTY=0.580184
PRIMER_LEFT_SEQUENCE=GGGTTTGGATGTAGCAGAATGT
PRIMER_RIGHT_SEQUENCE=CGTCTTCAAGAAGTGTCGGTTA
PRIMER_LEFT=74,22
PRIMER_RIGHT=337,22
PRIMER_LEFT_TM=60.249
PRIMER_RIGHT_TM=59.420
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.8828
PRIMER_LEFT_1_PENALTY=0.302618
PRIMER_RIGHT_1_PENALTY=0.580184
PRIMER_LEFT_1_SEQUENCE=GGAACGGTACAGTGCTAACCAT
PRIMER_RIGHT_1_SEQUENCE=CGTCTTCAAGAAGTGTCGGTTA
PRIMER_LEFT_1=175,22
PRIMER_RIGHT_1=337,22
PRIMER_LEFT_1_TM=60.303
PRIMER_RIGHT_1_TM=59.420
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=1.0814
PRIMER_LEFT_2_PENALTY=0.501224
PRIMER_RIGHT_2_PENALTY=0.580184
PRIMER_LEFT_2_SEQUENCE=GGTTTGGATGTAGCAGAATGTG
PRIMER_RIGHT_2_SEQUENCE=CGTCTTCAAGAAGTGTCGGTTA
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=337,22
PRIMER_LEFT_2_TM=59.499
PRIMER_RIGHT_2_TM=59.420
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=1.0955
PRIMER_LEFT_3_PENALTY=0.249074
PRIMER_RIGHT_3_PENALTY=0.846446
PRIMER_LEFT_3_SEQUENCE=GGGTTTGGATGTAGCAGAATGT
PRIMER_RIGHT_3_SEQUENCE=CTCGTCTTCAAGAAGTGTCGGT
PRIMER_LEFT_3=74,22
PRIMER_RIGHT_3=339,22
PRIMER_LEFT_3_TM=60.249
PRIMER_RIGHT_3_TM=60.846
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=1.0955
PRIMER_LEFT_4_PENALTY=0.249074
PRIMER_RIGHT_4_PENALTY=0.846446
PRIMER_LEFT_4_SEQUENCE=GGGTTTGGATGTAGCAGAATGT
PRIMER_RIGHT_4_SEQUENCE=ACCTCGTCTTCAAGAAGTGTCG
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=341,22
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=60.846
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_02181 Ps_contig_mira-and-tgicl-ass_franssen_19282
SEQUENCE=AAGAAGTGAAGGAAGAGGCGGAAGCAGTAGTTTGGTAGGTAGCCAATAGCAACTACTGAAAATGAGTCAGCACCACCACCACCCAGCAACTGCAGAGGATGTGATTGAGAACAAAACAAGTTGAATCAGTGGAAGCTCCAGCAGCAGAGGAAGTTCCAGTTGAGAATCCTGAAGCTTAAACCAATGGAATGGGTGTGACTGGAACTGGTAAAAAACGGAAGTTTAAATTAATGGTTCTAGATGTTGGGATCGGGTCTACCATTTTGCATGTCATCAAGGTTGAAAAGTCTTTTTCTATTTAATTTATTTCTTTAGTTTCCTTTCCTATATTAAAGTTTATTATTGTTATTATGGTATGGTCTTTTGGATATTGGTTTATTTATATGGCTACTTGGCCTCCACATCAGCATATATASRWSCKAATTTAAATTTAAA
TARGET=71,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02182 Ps_contig_mira-and-tgicl-ass_franssen_19283
SEQUENCE=AAATGAAAAATTAACAATAGGTGGGARTGAAGAAGAAGTCATGTGTAAGATATTATTATTGCAGAAATAAAAGTGTTCATGGAGATTCAAGAACTTAGATGCTTTTAATTTTTTAATTGGAACTTTGTTTTTTATTTATTTAATATTAGTAGTATGGTAGCTCAATGTGGAGCATGTGGATGATGTGATGTATGGTATTTCATGTGGTGCATGATGGCATGTAGTGACAATGTTTATGATATTTGTGCTCATAAAGATTGTGTCTTTTATATGCACTAATAGTAATCAAGHACCATGTGCATGGAATAGTAGAA
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02183 Ps_contig_mira-and-tgicl-ass_franssen_19296
SEQUENCE=TTCTTTGAGAGCAAAAGTGTTGAGATTGCTGGGTTTGCTCAGAATACAACAGTATGGACTTGGTGGAAATTCTCTTACTTCTGGTCAGAAAACTGTGCTTATTTATGGTACTTTGTTGTTTTTCTTTGGGCTTTTTCTTAGTGGCTACTTCATACAATAGTTATTATTATCATTCTTTTTTGTTTCTGAATTTATGTTGGATTTGATGCTTTGTTGGTCACAAGTGTATGTAATGTTGTCAATACTCAATAGTAAGAGCCAATTACAAACCATTTCCTTTGTAGAAATATGACTATGGAAGGAAATTGGTATTGCAATACTGCAATTCAATCATTCTCTTAGAAAGTGAAACTTACTCTCTCTCTCTATATGCTTGCAATTCAATCATTCTTTGACTATAAAAGG
TARGET=356,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02184 Ps_contig_mira-and-tgicl-ass_franssen_19306
SEQUENCE=AATGTTATTGCCAAAAGAGAATATCATTATTTTTATAATTTAAAGTTCACAATTACAACCATACATCACACATGGGCTGGGCCAGATTCCAACTTAAAAGTAATGAAAAAACTGCCCCATCACCCAACGCACTCACCACCTAATAATAATAATCATTACAACAACACGTTACACTTACAACAACATCCTTTACATACAAAAAGGGAAATAGTTAAAACAAGATAATGATCGAATTTCAAAATTAAAAAAACTAAAAAMMCCCCMCAACCAAAAGGTTTAGGGTTTTTTGGGTTTTTTAAAG
TARGET=141,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02185 Ps_contig_mira-and-tgicl-ass_franssen_19312
SEQUENCE=TTTCGCTTGGCTACTATCCAAACCGGAACCGGTATTAACCCTGCCAGAAGTTTCGGACCCGCAGTTATCTTCAACAACGACAAAGCTTGGGATGACCAGTGGATTTATTGGGTTGGACCATTTATTGGTGCTGCAGTGGCTGCAATCTACCACCAATACATTCTAAGAGGATCAGCAATTAAAGCTCTTGGATCCTTCAGGAGCAATGCTTAATGAATGCAACATGTTTGGCCTCAATTATTGTTATGAGTTGTTGATGTTGTTGTTGTTGTCTACTGGTCCCTGTTGAAGTTTTTGTCCTCTTGCTTTCAATTCCACGTTCTGAATGGGCCTGTGTGTTTGGAATAATTGGAATGGATAAAGAAGCCAAAATGATAATGGGGTCCTCAAAACTGTAGATAAGCATGAATATGATTATGATTATGGTTATGCTTATGTAATAAAACAAACTCAAATGTGACTTGAATTCCAATTTGTAGAAAACCCC
TARGET=258,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0368
PRIMER_LEFT_PENALTY=0.024665
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=GCTGCAATCTACCACCAATACA
PRIMER_RIGHT_SEQUENCE=AGGACAAAAACTTCAACAGGGA
PRIMER_LEFT=138,22
PRIMER_RIGHT=300,22
PRIMER_LEFT_TM=60.025
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.0368
PRIMER_LEFT_1_PENALTY=0.024665
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=GCTGCAATCTACCACCAATACA
PRIMER_RIGHT_1_SEQUENCE=GAGGACAAAAACTTCAACAGGG
PRIMER_LEFT_1=138,22
PRIMER_RIGHT_1=301,22
PRIMER_LEFT_1_TM=60.025
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.1391
PRIMER_LEFT_2_PENALTY=0.024665
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=GCTGCAATCTACCACCAATACA
PRIMER_RIGHT_2_SEQUENCE=GGACAAAAACTTCAACAGGGAC
PRIMER_LEFT_2=138,22
PRIMER_RIGHT_2=299,22
PRIMER_LEFT_2_TM=60.025
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=0.1391
PRIMER_LEFT_3_PENALTY=0.024665
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=GCTGCAATCTACCACCAATACA
PRIMER_RIGHT_3_SEQUENCE=GACAAAAACTTCAACAGGGACC
PRIMER_LEFT_3=138,22
PRIMER_RIGHT_3=298,22
PRIMER_LEFT_3_TM=60.025
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=0.1412
PRIMER_LEFT_4_PENALTY=0.024665
PRIMER_RIGHT_4_PENALTY=0.116582
PRIMER_LEFT_4_SEQUENCE=GCTGCAATCTACCACCAATACA
PRIMER_RIGHT_4_SEQUENCE=GTGGAATTGAAAGCAAGAGGAC
PRIMER_LEFT_4=138,22
PRIMER_RIGHT_4=317,22
PRIMER_LEFT_4_TM=60.025
PRIMER_RIGHT_4_TM=60.117
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=180
=
PRIMER_SEQUENCE_ID=SSR_02186 Ps_contig_mira-and-tgicl-ass_franssen_19313
SEQUENCE=TCAAATGATCCTGTCGGGTTTAACCTTGTTGATGTTTTGGCTTGGGGTTCTATAGGTCATATTGTTGCTTATTATATCTTGGCCACTTCTAGCAATGGTTATGATCCTAGTTTCTTTGGTTAATTAAGATTACTATGTTGTTCGTTTATGTTGTTTTAATTTGTCTTTTCTTGTCCCTTTTATGTTTATTAAGATGATGATGATGATGATGATGTATAGTTTATTTGTATGAGATATTCAACAAGTTAAGTTCCTTGGATTAAAGCATTTTGATTTGGATAAAAGCTTCTCTGGACTATGAAACCTTTTTTCTATATTGAAAATTATATTTAATTTTGTATTGTTTGACAATAATAAAATATA
TARGET=193,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5077
PRIMER_LEFT_PENALTY=0.142283
PRIMER_RIGHT_PENALTY=2.365428
PRIMER_LEFT_SEQUENCE=CGGGTTTAACCTTGTTGATGTT
PRIMER_RIGHT_SEQUENCE=GTCCAGAGAAGCTTTTATCCAA
PRIMER_LEFT=14,22
PRIMER_RIGHT=295,22
PRIMER_LEFT_TM=60.142
PRIMER_RIGHT_TM=57.635
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=2.7801
PRIMER_LEFT_1_PENALTY=0.142283
PRIMER_RIGHT_1_PENALTY=2.637844
PRIMER_LEFT_1_SEQUENCE=CGGGTTTAACCTTGTTGATGTT
PRIMER_RIGHT_1_SEQUENCE=AAAGGTTTCATAGTCCAGAGAAGC
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=307,24
PRIMER_LEFT_1_TM=60.142
PRIMER_RIGHT_1_TM=59.362
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=2.8378
PRIMER_LEFT_2_PENALTY=0.472356
PRIMER_RIGHT_2_PENALTY=2.365428
PRIMER_LEFT_2_SEQUENCE=GGCCACTTCTAGCAATGGTTAT
PRIMER_RIGHT_2_SEQUENCE=GTCCAGAGAAGCTTTTATCCAA
PRIMER_LEFT_2=80,22
PRIMER_RIGHT_2=295,22
PRIMER_LEFT_2_TM=59.528
PRIMER_RIGHT_2_TM=57.635
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=216
PRIMER_PAIR_PENALTY_3=2.9254
PRIMER_LEFT_3_PENALTY=0.559923
PRIMER_RIGHT_3_PENALTY=2.365428
PRIMER_LEFT_3_SEQUENCE=GGCTTGGGGTTCTATAGGTCAT
PRIMER_RIGHT_3_SEQUENCE=GTCCAGAGAAGCTTTTATCCAA
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=295,22
PRIMER_LEFT_3_TM=60.560
PRIMER_RIGHT_3_TM=57.635
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=2.9882
PRIMER_LEFT_4_PENALTY=0.622772
PRIMER_RIGHT_4_PENALTY=2.365428
PRIMER_LEFT_4_SEQUENCE=TCGGGTTTAACCTTGTTGATGT
PRIMER_RIGHT_4_SEQUENCE=GTCCAGAGAAGCTTTTATCCAA
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=295,22
PRIMER_LEFT_4_TM=60.623
PRIMER_RIGHT_4_TM=57.635
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_02187 Ps_contig_mira-and-tgicl-ass_franssen_19319
SEQUENCE=TTAATTATCGAGTTTGTAGGATGAGGATATTGATAAATTGAAAAAAAAAGTTTTTTTAGTTTTGTTTGAGTTAAAAAGTTAGAATTCTAATTATTATTACTATCGTTATATGTGATGATGATGAACATGATGATATGTAATGTTTGGGGTTTTATGCTGTACTCTATTATGGGATATCACAGTGATGTTGATATCGTGAATTGAATTTCAATGCACAAGAATAATTAATAGCTTGGTATTAAACY
TARGET=113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02188 Ps_contig_mira-and-tgicl-ass_franssen_19328
SEQUENCE=ATATATATACAAACAAAAAATAACTAATTTGAGTAAACAATTKAAAGGAAGCACAAAGAAATGCTTATACAGCAAGGTTGGATATCCAACCCAAATCTAATTTGAGCAGCTAATTTCCTCGAGTTTCCTCTTAACACTCATATCAATAAACTTAGAGCCAGTATTCCCATACCTGACAGTAAAACCAGCCACCAAACTTGGATCAAGAAGTGTTTTAGTCCTCACTTTCTTAGCCCCAGTAAGCTTCTGAACCTGTTTTGCAATCTGAGCCAAATGATGTGACTCCAACTTCACAACCGAAGTCACAACCACAAGCTCTGTATCAGTTAAAGTATTGTAAACAAATTCAAACTCCTTAATAATGTCGATGATCATGTCGATCCTCTTGGAGTCGATCAAGACGTTAAGGAAGTTGTGTGTGTGTGGTTGGAAGCCTGAGGTAGTGGCGAACTCTCCTATAAGTTGGCGTTTGGTGGAATCTTCGACTATTGGGCTGGAGAAATAGTCGAACACCTTGGGGTCAGAGAAAAGTTGTTCGATTTTGTCGAAGTCGGCGGTTATTGCGTCGAGGGTGTTGTTTGAATTTGCTAAGTCGGCTAAAGCGGCTGCGTAGCTGCCGGCGGCTAGGGATGACATTCTGGCTCCGAGGGCTCCGCCGGTGGAGCGGGTCGGGTTTTTGGGTGGAGGGGGGTAGGTTTTGGTAGTTTTGGTAGTTTTGTAGGTTTTGGGGAGTAGAAGGTAGAAAAGGAGGAGGTTGGAGGTAGTTGGGTTTTCGG
TARGET=414,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02189 Ps_contig_mira-and-tgicl-ass_franssen_19331
SEQUENCE=GACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGACCGTAGCCTCCATGTCCATGACCATAGCCATGACCATAACCTCCACCGTAATATTTGGCATCATTTACTTSATYTGACTTTTCATTGACGGACGGACGGGATCTGTTTGATTTAAATTCTGAGTAGCGTTTTATTTTGTTTTTCATGTGTCTATTTATACTTTTTCGTTCATGTGTTTGTTGATTCGACGGACGGGATCGATCTTCATGATTATGATGATGATTTATTTTGTGATTCATTTTGCTTTGGTGTTCACACGTGTATGTAGATGACCGTGATGATTTCATTGTACATCCATTAATTCATAGAATAAAATTGTTTGTCATTCTTTTTAATMAAAAAAMCTTTTTT
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02190 Ps_contig_mira-and-tgicl-ass_franssen_19331
SEQUENCE=GACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGACCGTAGCCTCCATGTCCATGACCATAGCCATGACCATAACCTCCACCGTAATATTTGGCATCATTTACTTSATYTGACTTTTCATTGACGGACGGACGGGATCTGTTTGATTTAAATTCTGAGTAGCGTTTTATTTTGTTTTTCATGTGTCTATTTATACTTTTTCGTTCATGTGTTTGTTGATTCGACGGACGGGATCGATCTTCATGATTATGATGATGATTTATTTTGTGATTCATTTTGCTTTGGTGTTCACACGTGTATGTAGATGACCGTGATGATTTCATTGTACATCCATTAATTCATAGAATAAAATTGTTTGTCATTCTTTTTAATMAAAAAAMCTTTTTT
TARGET=170,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02191 Ps_contig_mira-and-tgicl-ass_franssen_19332
SEQUENCE=TTAAACTAACTAGGACGTTATGTCATAGGTGGTGMGGTATACACACGYATGAGAKAYTGAAGAAGTTAGRTGAAARRAACAAAAAGAAACAACATGGAAGCTGTTTGGTTGGAGAGATAGATAAGCACTATAAGGCCTTTAAGCTCACTCTTGAAGTAGTTGATAAGGCTGATGGCACTGCTGTTGCTAAATGGACTGTTGAATATGAGAAAATCAATGAGGATATTCATCCTCCAAATGGATGGATGGACTATCTTAACCAATGCACTAGAGATATTGATGGTCATCTTATCAAAAGAGAAAACGTTGCTATATGAGTCATTCAGGCATTATAGTAATTAAATAAAGAGAGTGTTTAATTATTATTAGCGTGATGTTTGTAATGACATGCATGTAACCTATATAAGCATACATGTTTACTTCTCTTGATGCTTCCTTTGTATGGAATAAATGTGTCGGTCATGTCATGATGAATGGGTCTTATTATATTATTGTTGTAATGCAAATTAAATATTTCTGAATTATATAAGTTTGGTGTGCCAAATAACACATTAACTAA
TARGET=238,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02192 Ps_contig_mira-and-tgicl-ass_franssen_19333
SEQUENCE=TTTACAGTAAAACGAATATACANAATTTATAACAAACAAACACTATATTGTCTCAATATTCATCCCAAACAGCATAGTCTTTACCAGAAGCAACAACATGGAAATTTGATGGAACAAGATTTCCAAGATCCATCTTTGGCCCTATCTTCACAATAACCTTGTTGTCTATCTTTGCTACATATAAGTCACCATCAGCAGCTAGAATGTTCACACTGCTTTTCATATTTATCCCATTCTTCTGTCTTATTGCAGCCAATTTTGTTATCTCATCCTTCAAACCCCATCAATGAAATGATCATAGAATATTGATGGTGTCCGGATGTGTTAGAATATACGCATAACCCTGAATGACTTTATCAGAAGGAATGGCCAAAGTCTCTGAGTTGAACCTGTATCATGGTTATACAAATAAACGTTACACCTAGTTTTTTACGGGTTTTANACCGATCAATCCAGGTTGGTTTTCCGTGAATCTTCACTC
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02193 Ps_contig_mira-and-tgicl-ass_franssen_19344
SEQUENCE=GACATAACCAAAATACACTCATAAGAAAAACAAATGAGGTCCCTAATGGCTTCTTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATAATCAGCAAACCAATATTCTTATTCCTCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGTTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCTCCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTATCCCCATCCTCACCCTGTCTACCATTCCCCACCACCATCACATAAGAAACCATACAAGTACTNATCTCCCCCACCACCACCAGCAACTGCAGAGGATGTGATTGAGAACAAA
TARGET=345,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2909
PRIMER_LEFT_PENALTY=0.028056
PRIMER_RIGHT_PENALTY=0.262854
PRIMER_LEFT_SEQUENCE=CACCAGTTCACACCTATCCTCA
PRIMER_RIGHT_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_LEFT=201,22
PRIMER_RIGHT=476,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=60.263
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.4326
PRIMER_LEFT_1_PENALTY=0.169745
PRIMER_RIGHT_1_PENALTY=0.262854
PRIMER_LEFT_1_SEQUENCE=CACCCAGTCTACCACTCTCCTC
PRIMER_RIGHT_1_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_LEFT_1=278,22
PRIMER_RIGHT_1=476,22
PRIMER_LEFT_1_TM=60.170
PRIMER_RIGHT_1_TM=60.263
PRIMER_LEFT_1_GC_PERCENT=59.091
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=199
PRIMER_PAIR_PENALTY_2=0.4623
PRIMER_LEFT_2_PENALTY=0.215550
PRIMER_RIGHT_2_PENALTY=0.246726
PRIMER_LEFT_2_SEQUENCE=ACCTCCACCAGTTCACACCTAT
PRIMER_RIGHT_2_SEQUENCE=AATGGTAGACAGGGTGAGGATG
PRIMER_LEFT_2=196,22
PRIMER_RIGHT_2=392,22
PRIMER_LEFT_2_TM=59.784
PRIMER_RIGHT_2_TM=60.247
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=197
PRIMER_PAIR_PENALTY_3=0.4662
PRIMER_LEFT_3_PENALTY=0.203343
PRIMER_RIGHT_3_PENALTY=0.262854
PRIMER_LEFT_3_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_RIGHT_3_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_LEFT_3=309,22
PRIMER_RIGHT_3=476,22
PRIMER_LEFT_3_TM=60.203
PRIMER_RIGHT_3_TM=60.263
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=0.4662
PRIMER_LEFT_4_PENALTY=0.203343
PRIMER_RIGHT_4_PENALTY=0.262854
PRIMER_LEFT_4_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_RIGHT_4_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_LEFT_4=306,22
PRIMER_RIGHT_4=476,22
PRIMER_LEFT_4_TM=60.203
PRIMER_RIGHT_4_TM=60.263
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_02194 Ps_contig_mira-and-tgicl-ass_franssen_19344
SEQUENCE=GACATAACCAAAATACACTCATAAGAAAAACAAATGAGGTCCCTAATGGCTTCTTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATAATCAGCAAACCAATATTCTTATTCCTCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGTTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCTCCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTATCCCCATCCTCACCCTGTCTACCATTCCCCACCACCATCACATAAGAAACCATACAAGTACTNATCTCCCCCACCACCACCAGCAACTGCAGAGGATGTGATTGAGAACAAA
TARGET=438,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2909
PRIMER_LEFT_PENALTY=0.028056
PRIMER_RIGHT_PENALTY=0.262854
PRIMER_LEFT_SEQUENCE=CACCAGTTCACACCTATCCTCA
PRIMER_RIGHT_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_LEFT=201,22
PRIMER_RIGHT=476,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=60.263
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.4326
PRIMER_LEFT_1_PENALTY=0.169745
PRIMER_RIGHT_1_PENALTY=0.262854
PRIMER_LEFT_1_SEQUENCE=CACCCAGTCTACCACTCTCCTC
PRIMER_RIGHT_1_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_LEFT_1=278,22
PRIMER_RIGHT_1=476,22
PRIMER_LEFT_1_TM=60.170
PRIMER_RIGHT_1_TM=60.263
PRIMER_LEFT_1_GC_PERCENT=59.091
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=199
PRIMER_PAIR_PENALTY_2=0.4662
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.262854
PRIMER_LEFT_2_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_RIGHT_2_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_LEFT_2=309,22
PRIMER_RIGHT_2=476,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=60.263
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.4662
PRIMER_LEFT_3_PENALTY=0.203343
PRIMER_RIGHT_3_PENALTY=0.262854
PRIMER_LEFT_3_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_RIGHT_3_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_LEFT_3=306,22
PRIMER_RIGHT_3=476,22
PRIMER_LEFT_3_TM=60.203
PRIMER_RIGHT_3_TM=60.263
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.4784
PRIMER_LEFT_4_PENALTY=0.215550
PRIMER_RIGHT_4_PENALTY=0.262854
PRIMER_LEFT_4_SEQUENCE=ACCTCCACCAGTTCACACCTAT
PRIMER_RIGHT_4_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_LEFT_4=196,22
PRIMER_RIGHT_4=476,22
PRIMER_LEFT_4_TM=59.784
PRIMER_RIGHT_4_TM=60.263
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_02195 Ps_contig_mira-and-tgicl-ass_franssen_19351
SEQUENCE=ATAACTACCGTTTGGGTTCGTTCCCGGTTTTCGGTATTATTCTAGGWTGAHADGDCTGATGTTATTCTCAAATACATGCCGGATGAGGCTCGTCTTCTTAAGGCATATGGTGAGCTTCCTGATAATACTAGGCTTAATGAAGGTATTGGTGCTGGTCTTGATGAGGAAGATGATGGTGATGCTAATGATTATATCGAGTTTGAGGATGAGGATATTGAATAAAATTTAGTAAAAAAAAGTTTTTTTGTTTGTTTGAAAGTTAAAAAGTTGAATTCTAATTATTATTACTATCGTTATATGTGATGATGATGAACATGATGATWWGTAATGGTTTGGGTTTANGCTGTACTNTATTAG
TARGET=244,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02196 Ps_contig_mira-and-tgicl-ass_franssen_19351
SEQUENCE=ATAACTACCGTTTGGGTTCGTTCCCGGTTTTCGGTATTATTCTAGGWTGAHADGDCTGATGTTATTCTCAAATACATGCCGGATGAGGCTCGTCTTCTTAAGGCATATGGTGAGCTTCCTGATAATACTAGGCTTAATGAAGGTATTGGTGCTGGTCTTGATGAGGAAGATGATGGTGATGCTAATGATTATATCGAGTTTGAGGATGAGGATATTGAATAAAATTTAGTAAAAAAAAGTTTTTTTGTTTGTTTGAAAGTTAAAAAGTTGAATTCTAATTATTATTACTATCGTTATATGTGATGATGATGAACATGATGATWWGTAATGGTTTGGGTTTANGCTGTACTNTATTAG
TARGET=301,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02197 Ps_contig_mira-and-tgicl-ass_franssen_19355
SEQUENCE=GGAGGGAGGACGACGTTATATTGTTAACGGGTCGGTGGTGAATAACGGTGGAGGAGGAGATTATATTTGTAAATTAGGTGGTGGAATAGACAGGTGGNGGAGGAGACTATATTGTAATCGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTGTAATTGGTGGTGCATAGACAGGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTGACTTGTATTTGTAAACTGGTGGTGGAGGAGAAGGATATTTGTATGGCTTCTCTGACGGTGGTGGTGGTGAATTGTAGGCTCATCAAGAAGCAACATATCAGGCTCTATAAACAGTGCACGAGCAAGAGCTATACGCATCCTCCACCCTCCAGAAAATGTTTTTGTTGCCTTCTTC
TARGET=240,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1351
PRIMER_LEFT_PENALTY=0.117281
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=TAATTGGTGGTGCATAGACAGG
PRIMER_RIGHT_SEQUENCE=TTGCTTCTTGATGAGCCTACAA
PRIMER_LEFT=160,22
PRIMER_RIGHT=456,22
PRIMER_LEFT_TM=59.883
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.1734
PRIMER_LEFT_1_PENALTY=0.173354
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_1_SEQUENCE=TGATGAGCCTACAATTCACCAC
PRIMER_LEFT_1=207,22
PRIMER_RIGHT_1=448,22
PRIMER_LEFT_1_TM=59.827
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.1912
PRIMER_LEFT_2_PENALTY=0.173354
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_2_SEQUENCE=TTGCTTCTTGATGAGCCTACAA
PRIMER_LEFT_2=207,22
PRIMER_RIGHT_2=456,22
PRIMER_LEFT_2_TM=59.827
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.2108
PRIMER_LEFT_3_PENALTY=0.173354
PRIMER_RIGHT_3_PENALTY=0.037486
PRIMER_LEFT_3_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_3_SEQUENCE=CGTGCACTGTTTATAGAGCCTG
PRIMER_LEFT_3=207,22
PRIMER_RIGHT_3=483,22
PRIMER_LEFT_3_TM=59.827
PRIMER_RIGHT_3_TM=59.963
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.2608
PRIMER_LEFT_4_PENALTY=0.260719
PRIMER_RIGHT_4_PENALTY=0.000037
PRIMER_LEFT_4_SEQUENCE=GACAGGTGGAGGAGGAGACTTA
PRIMER_RIGHT_4_SEQUENCE=TGATGAGCCTACAATTCACCAC
PRIMER_LEFT_4=176,22
PRIMER_RIGHT_4=448,22
PRIMER_LEFT_4_TM=59.739
PRIMER_RIGHT_4_TM=60.000
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_02198 Ps_contig_mira-and-tgicl-ass_franssen_19355
SEQUENCE=GGAGGGAGGACGACGTTATATTGTTAACGGGTCGGTGGTGAATAACGGTGGAGGAGGAGATTATATTTGTAAATTAGGTGGTGGAATAGACAGGTGGNGGAGGAGACTATATTGTAATCGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTGTAATTGGTGGTGCATAGACAGGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTGACTTGTATTTGTAAACTGGTGGTGGAGGAGAAGGATATTTGTATGGCTTCTCTGACGGTGGTGGTGGTGAATTGTAGGCTCATCAAGAAGCAACATATCAGGCTCTATAAACAGTGCACGAGCAAGAGCTATACGCATCCTCCACCCTCCAGAAAATGTTTTTGTTGCCTTCTTC
TARGET=271,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1351
PRIMER_LEFT_PENALTY=0.117281
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=TAATTGGTGGTGCATAGACAGG
PRIMER_RIGHT_SEQUENCE=TTGCTTCTTGATGAGCCTACAA
PRIMER_LEFT=160,22
PRIMER_RIGHT=456,22
PRIMER_LEFT_TM=59.883
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.1734
PRIMER_LEFT_1_PENALTY=0.173354
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_1_SEQUENCE=TGATGAGCCTACAATTCACCAC
PRIMER_LEFT_1=207,22
PRIMER_RIGHT_1=448,22
PRIMER_LEFT_1_TM=59.827
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.1912
PRIMER_LEFT_2_PENALTY=0.173354
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_2_SEQUENCE=TTGCTTCTTGATGAGCCTACAA
PRIMER_LEFT_2=207,22
PRIMER_RIGHT_2=456,22
PRIMER_LEFT_2_TM=59.827
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.2108
PRIMER_LEFT_3_PENALTY=0.173354
PRIMER_RIGHT_3_PENALTY=0.037486
PRIMER_LEFT_3_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_3_SEQUENCE=CGTGCACTGTTTATAGAGCCTG
PRIMER_LEFT_3=207,22
PRIMER_RIGHT_3=483,22
PRIMER_LEFT_3_TM=59.827
PRIMER_RIGHT_3_TM=59.963
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.2608
PRIMER_LEFT_4_PENALTY=0.260719
PRIMER_RIGHT_4_PENALTY=0.000037
PRIMER_LEFT_4_SEQUENCE=GACAGGTGGAGGAGGAGACTTA
PRIMER_RIGHT_4_SEQUENCE=TGATGAGCCTACAATTCACCAC
PRIMER_LEFT_4=176,22
PRIMER_RIGHT_4=448,22
PRIMER_LEFT_4_TM=59.739
PRIMER_RIGHT_4_TM=60.000
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_02199 Ps_contig_mira-and-tgicl-ass_franssen_19355
SEQUENCE=GGAGGGAGGACGACGTTATATTGTTAACGGGTCGGTGGTGAATAACGGTGGAGGAGGAGATTATATTTGTAAATTAGGTGGTGGAATAGACAGGTGGNGGAGGAGACTATATTGTAATCGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTGTAATTGGTGGTGCATAGACAGGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTGACTTGTATTTGTAAACTGGTGGTGGAGGAGAAGGATATTTGTATGGCTTCTCTGACGGTGGTGGTGGTGAATTGTAGGCTCATCAAGAAGCAACATATCAGGCTCTATAAACAGTGCACGAGCAAGAGCTATACGCATCCTCCACCCTCCAGAAAATGTTTTTGTTGCCTTCTTC
TARGET=421,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0923
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=ATATGGTGGAGGAGGTGAATTG
PRIMER_RIGHT_SEQUENCE=TTGCTTCTTGATGAGCCTACAA
PRIMER_LEFT=323,22
PRIMER_RIGHT=456,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.1087
PRIMER_LEFT_1_PENALTY=0.074466
PRIMER_RIGHT_1_PENALTY=0.034282
PRIMER_LEFT_1_SEQUENCE=ATATGGTGGAGGAGGTGAATTG
PRIMER_RIGHT_1_SEQUENCE=AGGATGCGTATAGCTCTTGCTC
PRIMER_LEFT_1=323,22
PRIMER_RIGHT_1=504,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.034
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=0.1120
PRIMER_LEFT_2_PENALTY=0.074466
PRIMER_RIGHT_2_PENALTY=0.037486
PRIMER_LEFT_2_SEQUENCE=ATATGGTGGAGGAGGTGAATTG
PRIMER_RIGHT_2_SEQUENCE=CGTGCACTGTTTATAGAGCCTG
PRIMER_LEFT_2=323,22
PRIMER_RIGHT_2=483,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.963
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=0.1265
PRIMER_LEFT_3_PENALTY=0.108684
PRIMER_RIGHT_3_PENALTY=0.017846
PRIMER_LEFT_3_SEQUENCE=TGGAGGTGGTGACTTGTATTTG
PRIMER_RIGHT_3_SEQUENCE=TTGCTTCTTGATGAGCCTACAA
PRIMER_LEFT_3=353,22
PRIMER_RIGHT_3=456,22
PRIMER_LEFT_3_TM=59.891
PRIMER_RIGHT_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=104
PRIMER_PAIR_PENALTY_4=0.1351
PRIMER_LEFT_4_PENALTY=0.117281
PRIMER_RIGHT_4_PENALTY=0.017846
PRIMER_LEFT_4_SEQUENCE=TAATTGGTGGTGCATAGACAGG
PRIMER_RIGHT_4_SEQUENCE=TTGCTTCTTGATGAGCCTACAA
PRIMER_LEFT_4=160,22
PRIMER_RIGHT_4=456,22
PRIMER_LEFT_4_TM=59.883
PRIMER_RIGHT_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_02200 Ps_contig_mira-and-tgicl-ass_franssen_19356
SEQUENCE=GTGGGTATCAACGCAGAGTACGTSGGGGATCTTTAAAAATTATCACTCTTAACTAGTCACATTACATCCTTTTCTTTTATGATGATGACCGTCATGATTTCAATAATACCAATCATTTTGATTCACACTTTATTTTATGTTGGATTTTCTGATGACATGCCACTTACCACTACACTTCCACAATCACCAACATCACCTCTCCCACATTTTCACTTGTACAATACAAAATTCCAATTCATATCCTTCATCTACACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCTAGGAGAAGATCTCCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACCGTCTCAGACATGGCCACCAACATACTGCAAGCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGATGGCATAAATGTGAACGATTTTGTTCCTATAAAGACAAGACTCAATGAAATTGGCCGGCGTTATTTAAAAGGANCATCAAGAAGATGCTAACATTCAATTATTGGGTTAATCAATGGGTATGCNCACGGTAACG
TARGET=353,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02201 Ps_contig_mira-and-tgicl-ass_franssen_19356
SEQUENCE=GTGGGTATCAACGCAGAGTACGTSGGGGATCTTTAAAAATTATCACTCTTAACTAGTCACATTACATCCTTTTCTTTTATGATGATGACCGTCATGATTTCAATAATACCAATCATTTTGATTCACACTTTATTTTATGTTGGATTTTCTGATGACATGCCACTTACCACTACACTTCCACAATCACCAACATCACCTCTCCCACATTTTCACTTGTACAATACAAAATTCCAATTCATATCCTTCATCTACACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCTAGGAGAAGATCTCCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACCGTCTCAGACATGGCCACCAACATACTGCAAGCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGATGGCATAAATGTGAACGATTTTGTTCCTATAAAGACAAGACTCAATGAAATTGGCCGGCGTTATTTAAAAGGANCATCAAGAAGATGCTAACATTCAATTATTGGGTTAATCAATGGGTATGCNCACGGTAACG
TARGET=395,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02202 Ps_contig_mira-and-tgicl-ass_franssen_19358
SEQUENCE=GTCGGGGGACCATTGATAAGAATACAATTATCAAAAGCATAACAATCTTTTCAATTTCATTGCAATATACACACACGATGGCCGCATCATCATCATCATCCATGGCTCTCTCTTCTCCAACCTTGGCTGGCAAGCAACTCAAGCTCGACCCCATCAAGCCAAGAAGTTGGGAGCTGCAAGGTTCACCATGAGGAAGTCTGCCTACCAACCGAGAAGTAGCTTCCTCTGGAAGCCCATGGTACGGACCAGACCGTGTTAAGTACTTAGGCCCATTCTCCGGTGAGTCTCCATCCTACTTGACTGGAGAGTTCCCCGGTGACTACGGTTGGGACACTGCCGGACTCTCTGCTGACCCA
TARGET=85,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8439
PRIMER_LEFT_PENALTY=0.627348
PRIMER_RIGHT_PENALTY=0.216588
PRIMER_LEFT_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_SEQUENCE=GGAAGCTACTTCTCGGTTGGTA
PRIMER_LEFT=59,22
PRIMER_RIGHT=223,22
PRIMER_LEFT_TM=59.373
PRIMER_RIGHT_TM=59.783
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=0.9348
PRIMER_LEFT_1_PENALTY=0.627348
PRIMER_RIGHT_1_PENALTY=0.307443
PRIMER_LEFT_1_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_1_SEQUENCE=CTACTTCTCGGTTGGTAGGCAG
PRIMER_LEFT_1=59,22
PRIMER_RIGHT_1=218,22
PRIMER_LEFT_1_TM=59.373
PRIMER_RIGHT_1_TM=60.307
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.9839
PRIMER_LEFT_2_PENALTY=0.627348
PRIMER_RIGHT_2_PENALTY=0.356592
PRIMER_LEFT_2_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_2_SEQUENCE=GTTGGTAGGCAGACTTCCTCAT
PRIMER_LEFT_2=59,22
PRIMER_RIGHT_2=208,22
PRIMER_LEFT_2_TM=59.373
PRIMER_RIGHT_2_TM=59.643
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=1.2812
PRIMER_LEFT_3_PENALTY=0.627348
PRIMER_RIGHT_3_PENALTY=0.653834
PRIMER_LEFT_3_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_3_SEQUENCE=AGGAAGCTACTTCTCGGTTGGT
PRIMER_LEFT_3=59,22
PRIMER_RIGHT_3=224,22
PRIMER_LEFT_3_TM=59.373
PRIMER_RIGHT_3_TM=60.654
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=1.3773
PRIMER_LEFT_4_PENALTY=0.627348
PRIMER_RIGHT_4_PENALTY=0.749986
PRIMER_LEFT_4_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_4_SEQUENCE=GGGAACTCTCCAGTCAAGTAGG
PRIMER_LEFT_4=59,22
PRIMER_RIGHT_4=312,22
PRIMER_LEFT_4_TM=59.373
PRIMER_RIGHT_4_TM=59.250
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_02203 Ps_contig_mira-and-tgicl-ass_franssen_19367
SEQUENCE=TTCTAAGAGGATCAGCAATTAAAGCTCTTTTGGTATTCCTTTCTAGGTAGTCAATGCTTAATGAATGCAACATGTTTGGCCTCAATTATTGTTATGAGTTGTTGATGTTGTTGTTGTTGTCTACTGGTCCCTGTTGAAGTTTTTGTCCTCTTGCTTTCAATTCCACGTTCTGAATGGGCCTGTGTGTTTGGAATAATTGGAATGGATAAAGAAGCCAAAATGATAATGGGGTCCTCAAAACTGTAGATAAGCATGAATATGATTATGATTATGGTTATGGTTATGTATGTTATGTTTAAGGGTGTATTTGTATCCCTCCATTTTATCTTTTGTTATCTTTTTTGTTTTTGTTATTTTATTCAAGGATTTCTCATGTGTTCTCTTTAATTTATGTCATAAGTTAGACTTTTTATCAGATTTTTTACTTTGTTTTTAGTGGTGTGTTTCAACTCAATCCTCTTATGCTTCTCAAATTTGCTTATTGTCAATTTTGTTTGTGTTTCTAAAATTCAACCATAGCTCAATAATTTAG
TARGET=106,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1954
PRIMER_LEFT_PENALTY=2.078863
PRIMER_RIGHT_PENALTY=0.116582
PRIMER_LEFT_SEQUENCE=GCAACATGTTTGGCCTCAATTA
PRIMER_RIGHT_SEQUENCE=GTGGAATTGAAAGCAAGAGGAC
PRIMER_LEFT=66,22
PRIMER_RIGHT=165,22
PRIMER_LEFT_TM=62.079
PRIMER_RIGHT_TM=60.117
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=2.2149
PRIMER_LEFT_1_PENALTY=2.078863
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=GCAACATGTTTGGCCTCAATTA
PRIMER_RIGHT_1_SEQUENCE=TCTACAGTTTTGAGGACCCCAT
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=246,22
PRIMER_LEFT_1_TM=62.079
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=2.3750
PRIMER_LEFT_2_PENALTY=2.078863
PRIMER_RIGHT_2_PENALTY=0.296177
PRIMER_LEFT_2_SEQUENCE=GCAACATGTTTGGCCTCAATTA
PRIMER_RIGHT_2_SEQUENCE=CAGTTTTGAGGACCCCATTATC
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=242,22
PRIMER_LEFT_2_TM=62.079
PRIMER_RIGHT_2_TM=59.704
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=2.3750
PRIMER_LEFT_3_PENALTY=2.078863
PRIMER_RIGHT_3_PENALTY=0.296177
PRIMER_LEFT_3_SEQUENCE=GCAACATGTTTGGCCTCAATTA
PRIMER_RIGHT_3_SEQUENCE=AGTTTTGAGGACCCCATTATCA
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=241,22
PRIMER_LEFT_3_TM=62.079
PRIMER_RIGHT_3_TM=59.704
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=2.4857
PRIMER_LEFT_4_PENALTY=2.078863
PRIMER_RIGHT_4_PENALTY=0.406861
PRIMER_LEFT_4_SEQUENCE=GCAACATGTTTGGCCTCAATTA
PRIMER_RIGHT_4_SEQUENCE=TGGAGGGATACAAATACACCCT
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=319,22
PRIMER_LEFT_4_TM=62.079
PRIMER_RIGHT_4_TM=59.593
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_02204 Ps_contig_mira-and-tgicl-ass_franssen_19372
SEQUENCE=AATAATAATAATAGAGTGTGTAGTGTGTTATGCATTAATTTTTATGCATCATGTAGTATTGATCAACCTTTCATTATTGTTTCAGCTGGGTCAGCTCCAGCTCCGACTGCCGGCGGGTCCCCACCCGGCCTAAAGGTAGGTGGTGAATCTGGTGATAAAGGAGGTGCAGGCAGAGTCTCCTTCACAGGGTTTTCTTTTGTACTCTTATTAGCATCCATGATGTTCAATTAGGAAAATCTTACTGTATTTAATTTATCTATGTAATTGGCTTGGCTACTGAGTTAGTATTTGATTGCGTGTACTTTCTTTTTCCATTAATTTGGTGTTATTACTATTAATTAATTTATTGTCACAAACTCTTGTTGAATATTCTTTTAGGTACTAGTAGACTATWGAAAACWAAWTTTAGWAAATRRAGTAAACGAAAGCCTACAAAGTTAG
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02205 Ps_contig_mira-and-tgicl-ass_franssen_19373
SEQUENCE=AAAACTTTAGTAAGAAAGGGTAAGGAACTACATGTTTTCTACAAGGTTTAGGTTTGGCATTTTTCTAAAGCTACTGCTGSTRRKAGAAAAGACTGTTGCTTTTCCTTGTTTTTAGTTAGTGCTAGTCCTGGTCTTCAGATTCTTGACTTTGCACTTTTTGCAAGCAATTTCTCTACTCCTTTGATTGCAAAGACCACTTTCCTTGATCCTGTTCTTATCAAGAAACTCAAGAGTGTTCTTGGTGGAAGCGGCTAATTAAGCCTTATTTATATCTATATATGTAATTTATTTTTCTTAAGTAATGCTTATGCTTATAATAGTTGTTATTTTATTCAACCTATTTTCTTTGTTATTATTTCTTTCTTCTTCTTCTTTCTAATTCTATTCCCTACTATGTTAATTAGTAATTTCCYTTYGTTATACTAATAGTGGAGCAATAAATTACG
TARGET=361,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02206 Ps_contig_mira-and-tgicl-ass_franssen_19377
SEQUENCE=TGTTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTACTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAGACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTGTGTACACTTTAACCG
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02207 Ps_contig_mira-and-tgicl-ass_franssen_19384
SEQUENCE=CGTTCACTGGTAATCTTGTTTTTAATATTATTATTACTCCACCTGCTCAAATACATGTCACGATATTCTTTATTAGTTATATCTGCAAACTCGTTCAACCCAACTCTATAACTGTGATTTTGAGCATTATGCTCATCTATGAATATTAAGTTATCCTTAAATATTTGAAACCTTTGATTTTTCTCTCCTAATCCGTTATACACTTTTTGGTGTTTTACTAACCATTTCTCGTACATGGTCATAACCTCCTTGTTACTACGTCCACTTGACATGTCTAATGATAATGACAAAGTGATCAAGACAAAATAAGATCAAAGAGTATAGTATTGMYGMCWWGTAGTTGGTTGGTTTGGACTTAAAGTTAAAGGGAAGGAAGGAAAGGGTGGTAGTTAGGTCTTTAGAACGTTAGGTTAGGTATTTAGTTTTGGTCGTCCGCCCGTCGTACTCGT
TARGET=368,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02208 Ps_contig_mira-and-tgicl-ass_franssen_19393
SEQUENCE=ACCACCAGTGCATACCTACCCTCATCCTCACCCAGTCTACCATTCCCCGCCACCACCACCAACACCACACATATATCTGTGCATACACCTACCACCACCACTGTTTTGATCTTATTCTTCTTCACCCAATCTACAAAAACATTTGAACCATCTTCTTCCATTGAGCCAACATATCCGTCAAAACAATCCTTACGTCTGATTGTTACATTGGTCTCATTCTCTAGCCATCTTAATGCTGGAACTAGATTTGATTCATCAGTCCCAGCAATACAGTGAGGGGGATAAGGCTCCTCTGGCTTGTTAGGCTGGTGAGAATCAAGGAAAGCCATACTGGTAATTTCTTCTCACAGAACAGTCTCGCTAGCCTTGCTGATTCACTGATCTATTTCTAGTAAATTTTGTTTATAGACTTCTCGTTTGGGTVCCCAGTACTTTTCCMGACCCAAACCCCTAACGTTGGTACGG
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02209 Ps_contig_mira-and-tgicl-ass_franssen_19400
SEQUENCE=CTACATTTGNKAAAACAAAGTTTGAAGACAGTAAGAATCAAAGAATGGAAGTTCAAGAGAAGAGTATTGGACCACTGAAACTCAAACTAGCTAAGACAGAATCAGATGCAAAAATCACAATTGGACTTGCACCTGCTGAACTTGTTGTGGTTAATCCAAACATTAAAACTGCTGTTGGAAACGAAGTTGGTTATCGTTTGATTCCAGCAATTCCAGCTCATCCACTATTAACAGAGGATGATTATCCACAAATACGTGGTGCTTTTACAAACTATAATGTTTGGGTGACAGCATATAATAGAACTGAAAAATGGGCGGGTGGACTTTACGTTGATCGTAGTCGCGGTGATGATACTTTGGCAGTTTGGACTAAACAGAATAGAGAGATTGTGAACAAGGACATAGTGATGTGGCATGTAGTTGGAATTCATCATGTTCCAGCACAGGAAGATTTTCCAATAATGCCACTATTGAGCACTTCATTTGAGCTGAGGCCAACCAATTTCTTTGAGAGGAATCCAGTTCTTAAAACACTTTCACCAAGAGATGTTGCATGGCCTGGTTGCTCCAATTAAAACTAATCCGACATTCTGTATGGAACTGATTTGCATTGGCCTTGTGGTCATATGAATAAATGTTGTTTGTTTGTTGCATGTGGAAGAATAATGTATGGGTTTCAACTATTTTATTTGAATGAATGAATGAGAGGTTTATTTTCTTAGCTGAATTTTACAGTTTGTTTTTATGGGTAATATCTACTAAATATATATATGACACCAATAAG
TARGET=638,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02210 Ps_contig_mira-and-tgicl-ass_franssen_19400
SEQUENCE=CTACATTTGNKAAAACAAAGTTTGAAGACAGTAAGAATCAAAGAATGGAAGTTCAAGAGAAGAGTATTGGACCACTGAAACTCAAACTAGCTAAGACAGAATCAGATGCAAAAATCACAATTGGACTTGCACCTGCTGAACTTGTTGTGGTTAATCCAAACATTAAAACTGCTGTTGGAAACGAAGTTGGTTATCGTTTGATTCCAGCAATTCCAGCTCATCCACTATTAACAGAGGATGATTATCCACAAATACGTGGTGCTTTTACAAACTATAATGTTTGGGTGACAGCATATAATAGAACTGAAAAATGGGCGGGTGGACTTTACGTTGATCGTAGTCGCGGTGATGATACTTTGGCAGTTTGGACTAAACAGAATAGAGAGATTGTGAACAAGGACATAGTGATGTGGCATGTAGTTGGAATTCATCATGTTCCAGCACAGGAAGATTTTCCAATAATGCCACTATTGAGCACTTCATTTGAGCTGAGGCCAACCAATTTCTTTGAGAGGAATCCAGTTCTTAAAACACTTTCACCAAGAGATGTTGCATGGCCTGGTTGCTCCAATTAAAACTAATCCGACATTCTGTATGGAACTGATTTGCATTGGCCTTGTGGTCATATGAATAAATGTTGTTTGTTTGTTGCATGTGGAAGAATAATGTATGGGTTTCAACTATTTTATTTGAATGAATGAATGAGAGGTTTATTTTCTTAGCTGAATTTTACAGTTTGTTTTTATGGGTAATATCTACTAAATATATATATGACACCAATAAG
TARGET=691,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02211 Ps_contig_mira-and-tgicl-ass_franssen_19400
SEQUENCE=CTACATTTGNKAAAACAAAGTTTGAAGACAGTAAGAATCAAAGAATGGAAGTTCAAGAGAAGAGTATTGGACCACTGAAACTCAAACTAGCTAAGACAGAATCAGATGCAAAAATCACAATTGGACTTGCACCTGCTGAACTTGTTGTGGTTAATCCAAACATTAAAACTGCTGTTGGAAACGAAGTTGGTTATCGTTTGATTCCAGCAATTCCAGCTCATCCACTATTAACAGAGGATGATTATCCACAAATACGTGGTGCTTTTACAAACTATAATGTTTGGGTGACAGCATATAATAGAACTGAAAAATGGGCGGGTGGACTTTACGTTGATCGTAGTCGCGGTGATGATACTTTGGCAGTTTGGACTAAACAGAATAGAGAGATTGTGAACAAGGACATAGTGATGTGGCATGTAGTTGGAATTCATCATGTTCCAGCACAGGAAGATTTTCCAATAATGCCACTATTGAGCACTTCATTTGAGCTGAGGCCAACCAATTTCTTTGAGAGGAATCCAGTTCTTAAAACACTTTCACCAAGAGATGTTGCATGGCCTGGTTGCTCCAATTAAAACTAATCCGACATTCTGTATGGAACTGATTTGCATTGGCCTTGTGGTCATATGAATAAATGTTGTTTGTTTGTTGCATGTGGAAGAATAATGTATGGGTTTCAACTATTTTATTTGAATGAATGAATGAGAGGTTTATTTTCTTAGCTGAATTTTACAGTTTGTTTTTATGGGTAATATCTACTAAATATATATATGACACCAATAAG
TARGET=763,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02212 Ps_contig_mira-and-tgicl-ass_franssen_19404
SEQUENCE=AGGGTTTTTTTTTAAAGGTTTTAATTCATGATAAATTATTATTGTTGAAACACTAAACTGAAGTGATTTACAAAGAACTCAAGCTAACAAAACAAGCTAAGACTTTTGAGTTACAATTCAAGCAAAGATGATGAAAATGTTACAAGTAAACAACAACCATATCTTTCTGGTAGAAGTGTACACAAGTAAAAAAAGACTCACAACACATAAGAGATACTGTAATCTACAACCAATTATACTTTTCTTTCTTTCAATTAGTCTGTCTCAACACCTAATCCATACAACACACCAGAGTATTTCTCTGTTTCGCCATTGAAGAACGTATCCCTCAATTTTCTAAATGCACAAGCAGTGAGTAAACTATCCGATCCTGTCTTTGATGGGACCAAAACCAACCCTAAACTTTCCCTTTTTACCTAAAAA
TARGET=241,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.8874
PRIMER_LEFT_PENALTY=4.648778
PRIMER_RIGHT_PENALTY=0.238588
PRIMER_LEFT_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_SEQUENCE=AAAAGGGAAAGTTTAGGGTTGG
PRIMER_LEFT=153,25
PRIMER_RIGHT=412,22
PRIMER_LEFT_TM=58.351
PRIMER_RIGHT_TM=59.761
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=5.0038
PRIMER_LEFT_1_PENALTY=4.648778
PRIMER_RIGHT_1_PENALTY=0.354989
PRIMER_LEFT_1_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_1_SEQUENCE=GGGAAAGTTTAGGGTTGGTTTT
PRIMER_LEFT_1=153,25
PRIMER_RIGHT_1=408,22
PRIMER_LEFT_1_TM=58.351
PRIMER_RIGHT_1_TM=59.645
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=5.0200
PRIMER_LEFT_2_PENALTY=4.648778
PRIMER_RIGHT_2_PENALTY=0.371199
PRIMER_LEFT_2_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_2_SEQUENCE=ATACGTTCTTCAATGGCGAAAC
PRIMER_LEFT_2=153,25
PRIMER_RIGHT_2=324,22
PRIMER_LEFT_2_TM=58.351
PRIMER_RIGHT_2_TM=60.371
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=5.1252
PRIMER_LEFT_3_PENALTY=4.648778
PRIMER_RIGHT_3_PENALTY=0.476404
PRIMER_LEFT_3_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_3_SEQUENCE=AGTTTAGGGTTGGTTTTGGTCC
PRIMER_LEFT_3=153,25
PRIMER_RIGHT_3=403,22
PRIMER_LEFT_3_TM=58.351
PRIMER_RIGHT_3_TM=60.476
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=5.3320
PRIMER_LEFT_4_PENALTY=4.648778
PRIMER_RIGHT_4_PENALTY=0.683230
PRIMER_LEFT_4_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_4_SEQUENCE=AGGGAAAGTTTAGGGTTGGTTT
PRIMER_LEFT_4=153,25
PRIMER_RIGHT_4=409,22
PRIMER_LEFT_4_TM=58.351
PRIMER_RIGHT_4_TM=59.317
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_02213 Ps_contig_mira-and-tgicl-ass_franssen_19413
SEQUENCE=CATGCAAAGGGTGGTGGTGCACTTAGTGCTGTTGCGGCTACAAAGAGTCCTGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGCGGTGGACACTATGGTGGTGGACACTATGGAGGTGGACACTATGGTGGTGGTCACTACGGTCATGGTGG
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9462
PRIMER_LEFT_PENALTY=0.680486
PRIMER_RIGHT_PENALTY=0.265715
PRIMER_LEFT_SEQUENCE=CGGCTACAAAGAGTCCTGTCAT
PRIMER_RIGHT_SEQUENCE=CTCCATAGTGTCCACCACCATA
PRIMER_LEFT=34,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=60.680
PRIMER_RIGHT_TM=59.734
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.9462
PRIMER_LEFT_1_PENALTY=0.680486
PRIMER_RIGHT_1_PENALTY=0.265715
PRIMER_LEFT_1_SEQUENCE=CGGCTACAAAGAGTCCTGTCAT
PRIMER_RIGHT_1_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT_1=34,22
PRIMER_RIGHT_1=236,22
PRIMER_LEFT_1_TM=60.680
PRIMER_RIGHT_1_TM=59.734
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.9764
PRIMER_LEFT_2_PENALTY=0.680486
PRIMER_RIGHT_2_PENALTY=0.295944
PRIMER_LEFT_2_SEQUENCE=CGGCTACAAAGAGTCCTGTCAT
PRIMER_RIGHT_2_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_2=34,22
PRIMER_RIGHT_2=230,22
PRIMER_LEFT_2_TM=60.680
PRIMER_RIGHT_2_TM=60.296
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=197
PRIMER_PAIR_PENALTY_3=1.3024
PRIMER_LEFT_3_PENALTY=0.680486
PRIMER_RIGHT_3_PENALTY=0.621889
PRIMER_LEFT_3_SEQUENCE=CGGCTACAAAGAGTCCTGTCAT
PRIMER_RIGHT_3_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_3=34,22
PRIMER_RIGHT_3=155,22
PRIMER_LEFT_3_TM=60.680
PRIMER_RIGHT_3_TM=59.378
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=1.3324
PRIMER_LEFT_4_PENALTY=1.066676
PRIMER_RIGHT_4_PENALTY=0.265715
PRIMER_LEFT_4_SEQUENCE=CTGTTGCGGCTACAAAGAGTC
PRIMER_RIGHT_4_SEQUENCE=CTCCATAGTGTCCACCACCATA
PRIMER_LEFT_4=28,21
PRIMER_RIGHT_4=221,22
PRIMER_LEFT_4_TM=60.067
PRIMER_RIGHT_4_TM=59.734
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_02214 Ps_contig_mira-and-tgicl-ass_franssen_19413
SEQUENCE=CATGCAAAGGGTGGTGGTGCACTTAGTGCTGTTGCGGCTACAAAGAGTCCTGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGCGGTGGACACTATGGTGGTGGACACTATGGAGGTGGACACTATGGTGGTGGTCACTACGGTCATGGTGG
TARGET=84,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9462
PRIMER_LEFT_PENALTY=0.680486
PRIMER_RIGHT_PENALTY=0.265715
PRIMER_LEFT_SEQUENCE=CGGCTACAAAGAGTCCTGTCAT
PRIMER_RIGHT_SEQUENCE=CTCCATAGTGTCCACCACCATA
PRIMER_LEFT=34,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=60.680
PRIMER_RIGHT_TM=59.734
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.9462
PRIMER_LEFT_1_PENALTY=0.680486
PRIMER_RIGHT_1_PENALTY=0.265715
PRIMER_LEFT_1_SEQUENCE=CGGCTACAAAGAGTCCTGTCAT
PRIMER_RIGHT_1_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT_1=34,22
PRIMER_RIGHT_1=236,22
PRIMER_LEFT_1_TM=60.680
PRIMER_RIGHT_1_TM=59.734
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.9764
PRIMER_LEFT_2_PENALTY=0.680486
PRIMER_RIGHT_2_PENALTY=0.295944
PRIMER_LEFT_2_SEQUENCE=CGGCTACAAAGAGTCCTGTCAT
PRIMER_RIGHT_2_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_2=34,22
PRIMER_RIGHT_2=230,22
PRIMER_LEFT_2_TM=60.680
PRIMER_RIGHT_2_TM=60.296
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=197
PRIMER_PAIR_PENALTY_3=1.3024
PRIMER_LEFT_3_PENALTY=0.680486
PRIMER_RIGHT_3_PENALTY=0.621889
PRIMER_LEFT_3_SEQUENCE=CGGCTACAAAGAGTCCTGTCAT
PRIMER_RIGHT_3_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_3=34,22
PRIMER_RIGHT_3=155,22
PRIMER_LEFT_3_TM=60.680
PRIMER_RIGHT_3_TM=59.378
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=1.3324
PRIMER_LEFT_4_PENALTY=1.066676
PRIMER_RIGHT_4_PENALTY=0.265715
PRIMER_LEFT_4_SEQUENCE=CTGTTGCGGCTACAAAGAGTC
PRIMER_RIGHT_4_SEQUENCE=CTCCATAGTGTCCACCACCATA
PRIMER_LEFT_4=28,21
PRIMER_RIGHT_4=221,22
PRIMER_LEFT_4_TM=60.067
PRIMER_RIGHT_4_TM=59.734
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_02215 Ps_contig_mira-and-tgicl-ass_franssen_19421
SEQUENCE=TAGTCTAACGCACGAGTAACGCGGGGATCCAACAACAACAACACTATCATCATCGTCTCATACTTTACCTTTCATTTCAGAGTTACAGCTACATAATCCATTTTAGTTACAGTTAATTAATTTCCAGTTCGATCCATCTTGTTAGTAGTGATCATCATGGCTGAGGAGAATCAGAACAAGTACGAGGAGACTACCTCCGCAACCAACTCTGAGACAGAGATCAAGGACAGAGGTGTTTTTGATTTTCTCGGTGGTAAGAAAAAGGATGAAGAACATAAGCCTGCTCAAGATGAGGCGATTGCCCACGAGTTTGGTCACAAGGTGACTTTGTATGAAGCAACCGTMSGAAACCAAAGTTGAAGAGGAGWAGGTAAAAGAAAAAGAAACACACCAGTCTCTTGGAGAAACTTC
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02216 Ps_contig_mira-and-tgicl-ass_franssen_19422
SEQUENCE=GTCACACTTATAAAGCTTGAAGTTACAAAACTAATCGAAAAATACAATTCATATATGATAGTGAAAGATCTTGTTTGAATTATAGTAAGATATTTGAAAATATTCTAACGTTAATGAAAATTGACTGATCAGAATTGGAGGCTGGAAGCGTGAGATCGTACGGTGAGGAACCGTCAGAGCCAGCGACGAGAGTAACGCGTAGTATCATGATCGTGAAACCGCCAGGTTACCAAAGTGGATCGCCTCCGGCTTCTCCCGCCGGTTCAGTTACTCCGGTGTCTCCGTTTTCCGGTAAGGTCGTTATTCAGTTATTTGTCTTTTTATATATATACTGTACTCTATTTCTGCGACAGTTAAACTTAAGTTCTAAATTTACAGAATCCATTACAGTAAAGGTGCTCCTAAATTTCGGGCAGGGTAAATTTGTCTTTATGCGAAATGGCCTAACTTTCAAAATTTTTAAATTAGATAATTTAAATTAATTACTAGTATTAGG
TARGET=322,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3734
PRIMER_LEFT_PENALTY=0.038090
PRIMER_RIGHT_PENALTY=0.335333
PRIMER_LEFT_SEQUENCE=GTTCAGTTACTCCGGTGTCTCC
PRIMER_RIGHT_SEQUENCE=AGTTAGGCCATTTCGCATAAAG
PRIMER_LEFT=261,22
PRIMER_RIGHT=448,22
PRIMER_LEFT_TM=60.038
PRIMER_RIGHT_TM=59.665
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.4431
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.335333
PRIMER_LEFT_1_SEQUENCE=TCCGGTAAGGTCGTTATTCAGT
PRIMER_RIGHT_1_SEQUENCE=AGTTAGGCCATTTCGCATAAAG
PRIMER_LEFT_1=287,22
PRIMER_RIGHT_1=448,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=59.665
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.4855
PRIMER_LEFT_2_PENALTY=0.038090
PRIMER_RIGHT_2_PENALTY=0.447441
PRIMER_LEFT_2_SEQUENCE=GTTCAGTTACTCCGGTGTCTCC
PRIMER_RIGHT_2_SEQUENCE=GTTAGGCCATTTCGCATAAAGA
PRIMER_LEFT_2=261,22
PRIMER_RIGHT_2=447,22
PRIMER_LEFT_2_TM=60.038
PRIMER_RIGHT_2_TM=60.447
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=0.4855
PRIMER_LEFT_3_PENALTY=0.038090
PRIMER_RIGHT_3_PENALTY=0.447441
PRIMER_LEFT_3_SEQUENCE=GTTCAGTTACTCCGGTGTCTCC
PRIMER_RIGHT_3_SEQUENCE=TTAGGCCATTTCGCATAAAGAC
PRIMER_LEFT_3=261,22
PRIMER_RIGHT_3=446,22
PRIMER_LEFT_3_TM=60.038
PRIMER_RIGHT_3_TM=60.447
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=0.5552
PRIMER_LEFT_4_PENALTY=0.107763
PRIMER_RIGHT_4_PENALTY=0.447441
PRIMER_LEFT_4_SEQUENCE=TCCGGTAAGGTCGTTATTCAGT
PRIMER_RIGHT_4_SEQUENCE=GTTAGGCCATTTCGCATAAAGA
PRIMER_LEFT_4=287,22
PRIMER_RIGHT_4=447,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=60.447
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_02217 Ps_contig_mira-and-tgicl-ass_franssen_19426
SEQUENCE=ACCTCGCTTCAGGAAACCCTAGCTTATTGTAAACTAACTCTAGACTAATTCTTTCTTTCTAAGGGTTTTTTCCCTTAAAATTTAATTTCCGGTTCCCCTTAACTTTAGGTTGGGATAAATTGCTAGGAAGGTAATGTCTTGCAACTTCTTGGTTTGAGGAGCACCACTAACAACCCATGTATTAGCTGCAATGGATGCTTGAACTTTAGGGTTTGAGAACTGGATGACCACATCATCCTTAAAGATATTGACCTCCTCAATTTGAGGGATGGCATTCACCCCAATTCTCTTCAGAGTACTCTGAAGCCTTTTGTCGTCGGTAGTTGTTGTCTTGTGGACAGCCTTCTTCTTTCTTCTGACGGTACCCTTTCCACCAGTTCGAACTGAACCGGCCATCTTCATCAATTTTTCGCGATTCATCTTTGTGAAATCAGTGATTTTGGACAGGGTAAGCCTCGCCGTCCCCGCGTACTCTGTCG
TARGET=49,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6452
PRIMER_LEFT_PENALTY=1.644434
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=GCTTCAGGAAACCCTAGCTTATT
PRIMER_RIGHT_SEQUENCE=AACCCTAAAGTTCAAGCATCCA
PRIMER_LEFT=5,23
PRIMER_RIGHT=212,22
PRIMER_LEFT_TM=59.356
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=1.6569
PRIMER_LEFT_1_PENALTY=1.644434
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=GCTTCAGGAAACCCTAGCTTATT
PRIMER_RIGHT_1_SEQUENCE=TGTGGTCATCCAGTTCTCAAAC
PRIMER_LEFT_1=5,23
PRIMER_RIGHT_1=231,22
PRIMER_LEFT_1_TM=59.356
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=227
PRIMER_PAIR_PENALTY_2=1.6710
PRIMER_LEFT_2_PENALTY=1.644434
PRIMER_RIGHT_2_PENALTY=0.026532
PRIMER_LEFT_2_SEQUENCE=GCTTCAGGAAACCCTAGCTTATT
PRIMER_RIGHT_2_SEQUENCE=GATGTGGTCATCCAGTTCTCAA
PRIMER_LEFT_2=5,23
PRIMER_RIGHT_2=233,22
PRIMER_LEFT_2_TM=59.356
PRIMER_RIGHT_2_TM=59.973
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=1.7043
PRIMER_LEFT_3_PENALTY=1.644434
PRIMER_RIGHT_3_PENALTY=0.059875
PRIMER_LEFT_3_SEQUENCE=GCTTCAGGAAACCCTAGCTTATT
PRIMER_RIGHT_3_SEQUENCE=TCTGAAGAGAATTGGGGTGAAT
PRIMER_LEFT_3=5,23
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=59.356
PRIMER_RIGHT_3_TM=59.940
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=1.7546
PRIMER_LEFT_4_PENALTY=1.644434
PRIMER_RIGHT_4_PENALTY=0.110131
PRIMER_LEFT_4_SEQUENCE=GCTTCAGGAAACCCTAGCTTATT
PRIMER_RIGHT_4_SEQUENCE=ACTCTGAAGAGAATTGGGGTGA
PRIMER_LEFT_4=5,23
PRIMER_RIGHT_4=296,22
PRIMER_LEFT_4_TM=59.356
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_02218 Ps_contig_mira-and-tgicl-ass_franssen_19428
SEQUENCE=GAAAGAAAGAAACAATGATTGGTTTGAAAACGGAAACAAAGAAACAACACAGATAAATATCTTATCTTTATGCCACTGAATGGTTTAGTTTTATCAATCATGGCTTTCACTTCCTTTTTCCTCTTCTCCTCCCTTATCATCGCCGCCATCGCTCCCTCTTTGCATGCACAACCTAACCAGTTTTGTGAAGCTGGAATCGGATACGGAGACTCTTCCTGTGGAGATTCAATTCCATCATCATCCAAGTTGTTGATCAAAGGCGGTACTGTCGTTAATGCTCACCATCAACAAATCGCCGATGTTTATATTGAAGACGGTCTCATCGTTGCTGTTAATCCAACTATCACTGTTGGAGATGGAGGGTGCGTGTCATAGNTGCTACTGGACTAAGTTTGTANGCCGAGGGAGG
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02219 Ps_contig_mira-and-tgicl-ass_franssen_19429
SEQUENCE=CTAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTYRCGWATTGGCWSATACTTCAGTTGTTGCACATCTTGATTATTGTTAATCTTTTCCTCAGCCGGTTTCTGTTGAGGTTGAGATTCGGCTTGAGCTTCGGCGGCCATGATCGGACAATTGATCGGTGATGATTTGTGGTTTAGGTTTCAGGGGAATTTTGGGAATGAGAAAAATGGTGAGGTTGGATTGGGTTTTATATGAAAGGGAAAATGACGTCGTGGGAATAGGCGCAAATTTAGGGTAAGGGAAAAAACGAATGGTTGTGCTAAGAAAGCGAAAGAAAAACGCGTATATTTTCTTACTATGCTAAACAAAGACTTCTATTTTTTCTTCTTCGTTCCCCGA
TARGET=24,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02220 Ps_contig_mira-and-tgicl-ass_franssen_19431
SEQUENCE=GTAGTGGAGGAGCCAATAGCAACTACTGAAAATGAGTCAGCACCACCACCACCAGCAACTGCAGAGGATGTGATTGAGAACAAAACAGTTGAATCAGTGGAAGCTCCAGCAGCAGAGGAAGTTCCAGTTGAGAATCCTGAAGCTTAAACCAATGGAATGGGTGTGACTGGAACTAGGTAAAAAACGGAAGTTTAAATTAATGGTTCTAGATGGTTGGGATCGGGTCTACCTATTTGCATGTCATCGTAAGGGTTAGAAAAAGTTCCTTTTTTACTTAATTTTAAWTTTACTTTTTCTTTTACGTTTTCCCTTTACCTAATTTAATTAAAAGTTTATTTATTTGTTTATTAGTGGTATGGT
TARGET=41,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02221 Ps_contig_mira-and-tgicl-ass_franssen_19438
SEQUENCE=CCGTCGTCGTCGTTCGTCCTACCTTACCCTTTCCTTTCCTACTTACGGTACCGGTTTCGTTACCTTACCAACCTAACCTACTACTTCTACACGTGCACGTAGTCTTCTTTCTTCTTCTTTCCTTCTTCTTCTTTCTTTTCTCACCATCTTCTCCTTCCTCTTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGGTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCRCCYTGTGACCAAACTCGTGGGACAATCGCCTCATTCTTGAGCAGGCTTATGTTTCTTCTACTTCCTTTTTCCTTACCCACCGAAGAAAATACAAAAACCACCTCTGTTCCTTGTA
TARGET=2,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02222 Ps_contig_mira-and-tgicl-ass_franssen_19438
SEQUENCE=CCGTCGTCGTCGTTCGTCCTACCTTACCCTTTCCTTTCCTACTTACGGTACCGGTTTCGTTACCTTACCAACCTAACCTACTACTTCTACACGTGCACGTAGTCTTCTTTCTTCTTCTTTCCTTCTTCTTCTTTCTTTTCTCACCATCTTCTCCTTCCTCTTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGGTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCRCCYTGTGACCAAACTCGTGGGACAATCGCCTCATTCTTGAGCAGGCTTATGTTTCTTCTACTTCCTTTTTCCTTACCCACCGAAGAAAATACAAAAACCACCTCTGTTCCTTGTA
TARGET=122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02223 Ps_contig_mira-and-tgicl-ass_franssen_19446
SEQUENCE=AAAACTTTTTTAAAAAATTTAACTAAAATAGTCATTCTTATTGATTTTTTTGTTAGTCTTAGTAGTTCCCCCACGTGTCTCTAAAGCTTAAGAAAATAGATTATCATCCGGAAACATTTGAGACGAACACAATGAAATWGCAAATAACTTTGTAATAAAAAACAAATCTATTGCAAACAGGTCAAAGAGTGTAAATGAAATATTGCTGGACATCATGAAGAAGAAGAAGAATACAGTGGACGATCCTAACTCTAGAACACAACTGTGTCGAGCATCATTCCCAAAGTCTGAACAATGTTAGAAGACATTAAATTAGAAACGTGCTCTTCAAGATGCTTCTTAGCATCCTGCCTTCCTACATTAGCAATTGCAAGCTTTTCTGGGGGCAGCTCATCTTCCTCTTGCACTGCCTTGTTGTACTTGGTTGCTAAACTTAGCATCTCCTGAACAGTCTGTTCATTAGTTTTAGAATGTGAATCAAATCGTCTGAGTGTCAAACCATCGGTCCATTTCTTCTTATGAAGGTTAAGCAGCATTCTTCTCTTCAAGCTTCGTTCTTCYWGTTAATTGATTGCTTATTGAAGTAATATGTCGTCAANCAGTGGATCAAGGAGTCGTTAGGAATAAGTAGTTGGTTT
TARGET=217,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02224 Ps_contig_mira-and-tgicl-ass_franssen_19449
SEQUENCE=AGNGGTAGTCAACGCAGAGTMACDCGGGGCGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCATACCTACCCTCATCCTCACCCAGTCTACCATTCCCCGCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTCCTTACAAATATATATCTCCTCCTCCAACACCAATTTATAAATATAAGTCTCCTCCTCCACGTGTCTATGCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCTACCGGTTTACAAATATAAGTCTCCTCCTACCAAACCTAAGTACTATTCACCACCAATTTACAAATATAAATCT
TARGET=163,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02225 Ps_contig_mira-and-tgicl-ass_franssen_19449
SEQUENCE=AGNGGTAGTCAACGCAGAGTMACDCGGGGCGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCATACCTACCCTCATCCTCACCCAGTCTACCATTCCCCGCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTCCTTACAAATATATATCTCCTCCTCCAACACCAATTTATAAATATAAGTCTCCTCCTCCACGTGTCTATGCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCTACCGGTTTACAAATATAAGTCTCCTCCTACCAAACCTAAGTACTATTCACCACCAATTTACAAATATAAATCT
TARGET=375,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02226 Ps_contig_mira-and-tgicl-ass_franssen_19449
SEQUENCE=AGNGGTAGTCAACGCAGAGTMACDCGGGGCGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCATACCTACCCTCATCCTCACCCAGTCTACCATTCCCCGCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTCCTTACAAATATATATCTCCTCCTCCAACACCAATTTATAAATATAAGTCTCCTCCTCCACGTGTCTATGCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCTACCGGTTTACAAATATAAGTCTCCTCCTACCAAACCTAAGTACTATTCACCACCAATTTACAAATATAAATCT
TARGET=426,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02227 Ps_contig_mira-and-tgicl-ass_franssen_19449
SEQUENCE=AGNGGTAGTCAACGCAGAGTMACDCGGGGCGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCATACCTACCCTCATCCTCACCCAGTCTACCATTCCCCGCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTCCTTACAAATATATATCTCCTCCTCCAACACCAATTTATAAATATAAGTCTCCTCCTCCACGTGTCTATGCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCTACCGGTTTACAAATATAAGTCTCCTCCTACCAAACCTAAGTACTATTCACCACCAATTTACAAATATAAATCT
TARGET=468,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02228 Ps_contig_mira-and-tgicl-ass_franssen_19449
SEQUENCE=AGNGGTAGTCAACGCAGAGTMACDCGGGGCGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCATACCTACCCTCATCCTCACCCAGTCTACCATTCCCCGCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTCCTTACAAATATATATCTCCTCCTCCAACACCAATTTATAAATATAAGTCTCCTCCTCCACGTGTCTATGCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCTACCGGTTTACAAATATAAGTCTCCTCCTACCAAACCTAAGTACTATTCACCACCAATTTACAAATATAAATCT
TARGET=522,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02229 Ps_contig_mira-and-tgicl-ass_franssen_19449
SEQUENCE=AGNGGTAGTCAACGCAGAGTMACDCGGGGCGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCATACCTACCCTCATCCTCACCCAGTCTACCATTCCCCGCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTCCTTACAAATATATATCTCCTCCTCCAACACCAATTTATAAATATAAGTCTCCTCCTCCACGTGTCTATGCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCTACCGGTTTACAAATATAAGTCTCCTCCTACCAAACCTAAGTACTATTCACCACCAATTTACAAATATAAATCT
TARGET=565,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02230 Ps_contig_mira-and-tgicl-ass_franssen_19449
SEQUENCE=AGNGGTAGTCAACGCAGAGTMACDCGGGGCGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCATACCTACCCTCATCCTCACCCAGTCTACCATTCCCCGCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTCCTTACAAATATATATCTCCTCCTCCAACACCAATTTATAAATATAAGTCTCCTCCTCCACGTGTCTATGCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCTACCGGTTTACAAATATAAGTCTCCTCCTACCAAACCTAAGTACTATTCACCACCAATTTACAAATATAAATCT
TARGET=659,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02231 Ps_contig_mira-and-tgicl-ass_franssen_19449
SEQUENCE=AGNGGTAGTCAACGCAGAGTMACDCGGGGCGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCATACCTACCCTCATCCTCACCCAGTCTACCATTCCCCGCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTCCTTACAAATATATATCTCCTCCTCCAACACCAATTTATAAATATAAGTCTCCTCCTCCACGTGTCTATGCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCTACCGGTTTACAAATATAAGTCTCCTCCTACCAAACCTAAGTACTATTCACCACCAATTTACAAATATAAATCT
TARGET=691,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02232 Ps_contig_mira-and-tgicl-ass_franssen_19463
SEQUENCE=GTCGGGGGTCACACATAATACTATTGCAAGAAGTGTCACTCACAAAAACTATYCTCATTGTGTGACAAGAATCAGGCACAAAGATGGCTTGTAACACATTGATGAGTTCAGCTTTCTCAGCTTTTCCATCTCTTTCTTTCTTCCTCAAAATCAAGTATTTTCTAMYTCACTCCACTTCCTTATGTTGCTTCTGCCAATGTCTTCTTCTAGATTCACCTA
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02233 Ps_contig_mira-and-tgicl-ass_franssen_19464
SEQUENCE=AGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAATCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGATGGCATAAATGTGAACGATTTTGTTTCCTATAAAGACAAGACTCAATGAAATTGGCCGGCGTTATTTAAAAGGANCATCAAGAAGATGCAAACATTCATTATGGTTATNANGTNTGCTCACGGAACTTGTTTCAGATACAATTGTTTCTAAGTTTTATTTTTCTTTACTTTAGG
TARGET=7,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02234 Ps_contig_mira-and-tgicl-ass_franssen_19464
SEQUENCE=AGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAATCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGATGGCATAAATGTGAACGATTTTGTTTCCTATAAAGACAAGACTCAATGAAATTGGCCGGCGTTATTTAAAAGGANCATCAAGAAGATGCAAACATTCATTATGGTTATNANGTNTGCTCACGGAACTTGTTTCAGATACAATTGTTTCTAAGTTTTATTTTTCTTTACTTTAGG
TARGET=61,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0813
PRIMER_LEFT_PENALTY=1.016921
PRIMER_RIGHT_PENALTY=0.064372
PRIMER_LEFT_SEQUENCE=AGAAGATCTCCACCACCACCT
PRIMER_RIGHT_SEQUENCE=GGCCATAACCCATGGATAGTAA
PRIMER_LEFT=21,21
PRIMER_RIGHT=241,22
PRIMER_LEFT_TM=59.983
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=1.1478
PRIMER_LEFT_1_PENALTY=1.016921
PRIMER_RIGHT_1_PENALTY=0.130883
PRIMER_LEFT_1_SEQUENCE=AGAAGATCTCCACCACCACCT
PRIMER_RIGHT_1_SEQUENCE=CCATCTTTGTTGCAATCTCGTA
PRIMER_LEFT_1=21,21
PRIMER_RIGHT_1=283,22
PRIMER_LEFT_1_TM=59.983
PRIMER_RIGHT_1_TM=60.131
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=1.1567
PRIMER_LEFT_2_PENALTY=1.016921
PRIMER_RIGHT_2_PENALTY=0.139753
PRIMER_LEFT_2_SEQUENCE=AGAAGATCTCCACCACCACCT
PRIMER_RIGHT_2_SEQUENCE=TGCAATCTCGTATCTCAACACC
PRIMER_LEFT_2=21,21
PRIMER_RIGHT_2=273,22
PRIMER_LEFT_2_TM=59.983
PRIMER_RIGHT_2_TM=60.140
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=1.2799
PRIMER_LEFT_3_PENALTY=1.016921
PRIMER_RIGHT_3_PENALTY=0.263023
PRIMER_LEFT_3_SEQUENCE=AGAAGATCTCCACCACCACCT
PRIMER_RIGHT_3_SEQUENCE=TCTTTACTTGGCCATAACCCAT
PRIMER_LEFT_3=21,21
PRIMER_RIGHT_3=250,22
PRIMER_LEFT_3_TM=59.983
PRIMER_RIGHT_3_TM=59.737
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=1.7095
PRIMER_LEFT_4_PENALTY=1.016921
PRIMER_RIGHT_4_PENALTY=0.692533
PRIMER_LEFT_4_SEQUENCE=AGAAGATCTCCACCACCACCT
PRIMER_RIGHT_4_SEQUENCE=TTCTTTACTTGGCCATAACCCA
PRIMER_LEFT_4=21,21
PRIMER_RIGHT_4=251,22
PRIMER_LEFT_4_TM=59.983
PRIMER_RIGHT_4_TM=60.693
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_02235 Ps_contig_mira-and-tgicl-ass_franssen_19464
SEQUENCE=AGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAATCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGATGGCATAAATGTGAACGATTTTGTTTCCTATAAAGACAAGACTCAATGAAATTGGCCGGCGTTATTTAAAAGGANCATCAAGAAGATGCAAACATTCATTATGGTTATNANGTNTGCTCACGGAACTTGTTTCAGATACAATTGTTTCTAAGTTTTATTTTTCTTTACTTTAGG
TARGET=103,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3132
PRIMER_LEFT_PENALTY=0.248820
PRIMER_RIGHT_PENALTY=0.064372
PRIMER_LEFT_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_RIGHT_SEQUENCE=GGCCATAACCCATGGATAGTAA
PRIMER_LEFT=62,22
PRIMER_RIGHT=241,22
PRIMER_LEFT_TM=59.751
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=0.3797
PRIMER_LEFT_1_PENALTY=0.248820
PRIMER_RIGHT_1_PENALTY=0.130883
PRIMER_LEFT_1_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_RIGHT_1_SEQUENCE=CCATCTTTGTTGCAATCTCGTA
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=283,22
PRIMER_LEFT_1_TM=59.751
PRIMER_RIGHT_1_TM=60.131
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.3886
PRIMER_LEFT_2_PENALTY=0.248820
PRIMER_RIGHT_2_PENALTY=0.139753
PRIMER_LEFT_2_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_RIGHT_2_SEQUENCE=TGCAATCTCGTATCTCAACACC
PRIMER_LEFT_2=62,22
PRIMER_RIGHT_2=273,22
PRIMER_LEFT_2_TM=59.751
PRIMER_RIGHT_2_TM=60.140
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.5118
PRIMER_LEFT_3_PENALTY=0.248820
PRIMER_RIGHT_3_PENALTY=0.263023
PRIMER_LEFT_3_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_RIGHT_3_SEQUENCE=TCTTTACTTGGCCATAACCCAT
PRIMER_LEFT_3=62,22
PRIMER_RIGHT_3=250,22
PRIMER_LEFT_3_TM=59.751
PRIMER_RIGHT_3_TM=59.737
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.7159
PRIMER_LEFT_4_PENALTY=0.651564
PRIMER_RIGHT_4_PENALTY=0.064372
PRIMER_LEFT_4_SEQUENCE=CACCACCACCACCTAGGATAAG
PRIMER_RIGHT_4_SEQUENCE=GGCCATAACCCATGGATAGTAA
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=241,22
PRIMER_LEFT_4_TM=60.652
PRIMER_RIGHT_4_TM=59.936
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_02236 Ps_contig_mira-and-tgicl-ass_franssen_19480
SEQUENCE=ATGATGAAAATGTTACAAGTAAACAACAACCATATCTTTCTGGTAGAAGTGTACACAGTAAAAAAGAACTCACAACACATAAGAGATACTGTAATCTACAACCAATTATACTTTCTTTCTTTCTATATNTGTNTCACACCAACTACCACTACAACACACCACGAGTA
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=5.1801
PRIMER_LEFT_PENALTY=4.648778
PRIMER_RIGHT_PENALTY=0.531362
PRIMER_LEFT_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_SEQUENCE=ACTCGTGGTGTGTTGTAGTGGT
PRIMER_LEFT=26,25
PRIMER_RIGHT=165,22
PRIMER_LEFT_TM=58.351
PRIMER_RIGHT_TM=59.469
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=140
PRIMER_PAIR_PENALTY_1=5.7832
PRIMER_LEFT_1_PENALTY=4.648778
PRIMER_RIGHT_1_PENALTY=1.134408
PRIMER_LEFT_1_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_1_SEQUENCE=TGGTGTGTTGTAGTGGTAGTTGG
PRIMER_LEFT_1=26,25
PRIMER_RIGHT_1=160,23
PRIMER_LEFT_1_TM=58.351
PRIMER_RIGHT_1_TM=59.866
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=6.0147
PRIMER_LEFT_2_PENALTY=4.648778
PRIMER_RIGHT_2_PENALTY=1.365912
PRIMER_LEFT_2_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_2_SEQUENCE=CTCGTGGTGTGTTGTAGTGGTAG
PRIMER_LEFT_2=26,25
PRIMER_RIGHT_2=164,23
PRIMER_LEFT_2_TM=58.351
PRIMER_RIGHT_2_TM=59.634
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=52.174
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=6.0203
PRIMER_LEFT_3_PENALTY=4.648778
PRIMER_RIGHT_3_PENALTY=1.371570
PRIMER_LEFT_3_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_3_SEQUENCE=TCGTGGTGTGTTGTAGTGGTAG
PRIMER_LEFT_3=26,25
PRIMER_RIGHT_3=163,22
PRIMER_LEFT_3_TM=58.351
PRIMER_RIGHT_3_TM=58.628
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=6.0203
PRIMER_LEFT_4_PENALTY=4.648778
PRIMER_RIGHT_4_PENALTY=1.371570
PRIMER_LEFT_4_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_4_SEQUENCE=TACTCGTGGTGTGTTGTAGTGG
PRIMER_LEFT_4=26,25
PRIMER_RIGHT_4=166,22
PRIMER_LEFT_4_TM=58.351
PRIMER_RIGHT_4_TM=58.628
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_02237 Ps_contig_mira-and-tgicl-ass_franssen_19489
SEQUENCE=ATGTCCACCGTGTCCACCTCCACCATGGTTAACCACCACCACCATTATGGTAACCTCCACCGCCATTGTAACCACCTCCACCATTGTGGTAACCACCTCCATTATGGTAACCACCACCACGTCCATAACCTCCATATTTAGCATCATTTACTTCATTTGACTTTTCAACAACCTCCTCAGTTAATTCCCTAGCTGACACCTCTGATGAAATAAAGAAGAACCATGGCCAATAGGCCAAGAATGAGCATTGTCTTTTCTTGGAATCCATTGTACTGAACTCAAGAAGAGTTGAAATGTGATATTTGGTGNGAAGGACTCCCGCGTATCTGCGTTGACTCC
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02238 Ps_contig_mira-and-tgicl-ass_franssen_19493
SEQUENCE=TGGTCATATGCCACCAACTTTGTTCCCGGAAAATAAGCACTCTTATATTTATATGTTTTGTGATAGTAATTTTCTTCCCAATTCAATGTGATATTATCATATCATATCATGTGTGTATGC
TARGET=95,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02239 Ps_contig_mira-and-tgicl-ass_franssen_19516
SEQUENCE=TTCACACACGAGAGCTGGACCCATTACGCAACACACACACAACTTCCTTAACGTCTTGATCGACTCCAAGAGGATCGACATGATCATCGACATTATTAAGGAGTTTCTGAGACACGCAAC
TARGET=31,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0822
PRIMER_LEFT_PENALTY=2.029992
PRIMER_RIGHT_PENALTY=0.052187
PRIMER_LEFT_SEQUENCE=TTCACACACGAGAGCTGGAC
PRIMER_RIGHT_SEQUENCE=TGCGTGTCTCAGAAACTCCTTA
PRIMER_LEFT=0,20
PRIMER_RIGHT=117,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=118
PRIMER_PAIR_PENALTY_1=2.7462
PRIMER_LEFT_1_PENALTY=2.693987
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=ACACGAGAGCTGGACCCATTAC
PRIMER_RIGHT_1_SEQUENCE=TGCGTGTCTCAGAAACTCCTTA
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=117,22
PRIMER_LEFT_1_TM=62.694
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=2.8863
PRIMER_LEFT_2_PENALTY=2.834079
PRIMER_RIGHT_2_PENALTY=0.052187
PRIMER_LEFT_2_SEQUENCE=ACGAGAGCTGGACCCATTAC
PRIMER_RIGHT_2_SEQUENCE=TGCGTGTCTCAGAAACTCCTTA
PRIMER_LEFT_2=7,20
PRIMER_RIGHT_2=117,22
PRIMER_LEFT_2_TM=59.166
PRIMER_RIGHT_2_TM=60.052
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=2.8866
PRIMER_LEFT_3_PENALTY=2.029992
PRIMER_RIGHT_3_PENALTY=0.856575
PRIMER_LEFT_3_SEQUENCE=TTCACACACGAGAGCTGGAC
PRIMER_RIGHT_3_SEQUENCE=GTTGCGTGTCTCAGAAACTCCT
PRIMER_LEFT_3=0,20
PRIMER_RIGHT_3=119,22
PRIMER_LEFT_3_TM=60.030
PRIMER_RIGHT_3_TM=60.857
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=3.0267
PRIMER_LEFT_4_PENALTY=2.974471
PRIMER_RIGHT_4_PENALTY=0.052187
PRIMER_LEFT_4_SEQUENCE=CACGAGAGCTGGACCCATTAC
PRIMER_RIGHT_4_SEQUENCE=TGCGTGTCTCAGAAACTCCTTA
PRIMER_LEFT_4=6,21
PRIMER_RIGHT_4=117,22
PRIMER_LEFT_4_TM=61.974
PRIMER_RIGHT_4_TM=60.052
PRIMER_LEFT_4_GC_PERCENT=57.143
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=112
=
PRIMER_SEQUENCE_ID=SSR_02240 Ps_contig_mira-and-tgicl-ass_franssen_19587
SEQUENCE=CCTAAGTAAGGAAGTACAAAACTAARCCTCCTCMAYTATAACTAAAGAACAATACAAGMCAAACAAAACAAAATAGATGAGCAAGGAAGAATCAACATGACTTGCTACTAATACAGCTCTATTCGGTTCCGGCAATTTTCTTTTTCAGTGCCTCAATATCCGTAGCTAGTTCCTTTCTACTAGACTTAAACAGAAGGTACCGGTAGACAAACCATCCAGTGTACCCTAGTCCTACCAACTCCATAATCTTTGGAAGCAAGGGGACTGAGTTGATGGCACCTACAAGAATTGAAGATAACCAAACAGCAACCAAACCACCACCACCATACAGAAGCACTGTGGACTTGTTTTCAAGACCATCCCACTTCTCCTTCAAATCGGTAAACAACTCATTGGTATCGAGAAAGAAGTAGATGTATCATCAGAAGAA
TARGET=314,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02241 Ps_contig_mira-and-tgicl-ass_franssen_19590
SEQUENCE=GGGTTAATGGGTAGAACGAGACTCAAAATTTTCTGGTATATATATATGTCATGAAGAATGCAACTCTTCATCTCACATTTATTTACGTACAATATCATGGTTTATTAGAAATGTTGTGCTAATTGAAGAATGCAAAAGACATTTGATTGCCATGGAACCTAACAAGCTTCACATGCTAATATTACATTGACATTAACAGAGATTTGAAGGTTCTGATTCAATTCTTTATTACATGGAATACATTATATACATGCACCTACTACTTTACATATCAACAAGCAAGCGCGTTGACAGTTCAGAGATCCTCAACGTAGGGGAAAAANCCTTACCAAGTAAA
TARGET=37,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3926
PRIMER_LEFT_PENALTY=1.106446
PRIMER_RIGHT_PENALTY=0.286105
PRIMER_LEFT_SEQUENCE=GGGTTAATGGGTAGAACGAGAC
PRIMER_RIGHT_SEQUENCE=GCGCTTGCTTGTTGATATGTAA
PRIMER_LEFT=0,22
PRIMER_RIGHT=285,22
PRIMER_LEFT_TM=58.894
PRIMER_RIGHT_TM=60.286
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=1.4966
PRIMER_LEFT_1_PENALTY=1.210476
PRIMER_RIGHT_1_PENALTY=0.286105
PRIMER_LEFT_1_SEQUENCE=GGGTTAATGGGTAGAACGAGACT
PRIMER_RIGHT_1_SEQUENCE=GCGCTTGCTTGTTGATATGTAA
PRIMER_LEFT_1=0,23
PRIMER_RIGHT_1=285,22
PRIMER_LEFT_1_TM=59.790
PRIMER_RIGHT_1_TM=60.286
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=2.2988
PRIMER_LEFT_2_PENALTY=1.106446
PRIMER_RIGHT_2_PENALTY=1.192395
PRIMER_LEFT_2_SEQUENCE=GGGTTAATGGGTAGAACGAGAC
PRIMER_RIGHT_2_SEQUENCE=GTTCCATGGCAATCAAATGTC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=157,21
PRIMER_LEFT_2_TM=58.894
PRIMER_RIGHT_2_TM=60.192
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=158
PRIMER_PAIR_PENALTY_3=2.3200
PRIMER_LEFT_3_PENALTY=1.210476
PRIMER_RIGHT_3_PENALTY=1.109530
PRIMER_LEFT_3_SEQUENCE=GGGTTAATGGGTAGAACGAGACT
PRIMER_RIGHT_3_SEQUENCE=GTTCCATGGCAATCAAATGTCT
PRIMER_LEFT_3=0,23
PRIMER_RIGHT_3=157,22
PRIMER_LEFT_3_TM=59.790
PRIMER_RIGHT_3_TM=61.110
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=2.3392
PRIMER_LEFT_4_PENALTY=1.106446
PRIMER_RIGHT_4_PENALTY=1.232712
PRIMER_LEFT_4_SEQUENCE=GGGTTAATGGGTAGAACGAGAC
PRIMER_RIGHT_4_SEQUENCE=TGTGAAGCTTGTTAGGTTCCAT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=172,22
PRIMER_LEFT_4_TM=58.894
PRIMER_RIGHT_4_TM=58.767
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_02242 Ps_contig_mira-and-tgicl-ass_franssen_19593
SEQUENCE=CAACGCAGAGTACGGTCGGGGTTCACACAAATAAGAATAAGAACGAAGTTGCGAAACTAGGGTTTCCTTCTCCACACACACCAAATCGAATACCATCCAGAGACCAAAACCAGTCGAATTTGAAGCGCAATCATGCCGAAGAACAAGGGAAAGGGAGGAAAGAATCGAAAGAGGGGTAAGAACGAAGCCGACGATGAGAAACGCGAGCTTGTTTTCAAAGAAGATGGCCAAGAATATGCTCAAGTTCTCCGCATGTTAGGTAACGGTAGATGCGAAGCTATGTGCATCGACGGTACAAAGCGTCTCTGTCACATCCGTGGGAAGATGCACAAAAAGGTCTGGATTGCCGCCGGCGATATCATACTCGTTGGTCTCCGTGATTATCAGGATGATAAGGCTGATGTTATTCTCAAATACATGCCGGATGAGGCTCGTCTTCTTAAGGCGTATGGTGAGCTTCCTGATAATACTAGGCTTAATGAAGGTATTGGTGCTGGTCTTGATGAGGAAGATGATGGTGATGCTAATGATTATATCGAGTTTGAGGATGAGGATATTGATAAAATTTGAAAAAAAAGTTTTTTTTGTTTGTTTGAAGTTAAAAAGTTGAATTCTAATTATTATTACTATCGTTATATGTGATGATGATGAACATGATGATATGTAATGTTTGGGGTTTTATGCTGTACTCTATTATGGGATATCACAGTGATGTTGATATCGTGAATTGAATTTCAATGCACAAGAATAATTAATAGCTTGGTATTAAGAACTTAAAAAAAAAA
TARGET=584,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0782
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=1.078158
PRIMER_LEFT_SEQUENCE=GTATTGGTGCTGGTCTTGATGA
PRIMER_RIGHT_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT=484,22
PRIMER_RIGHT=688,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=58.922
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=1.1864
PRIMER_LEFT_1_PENALTY=0.108206
PRIMER_RIGHT_1_PENALTY=1.078158
PRIMER_LEFT_1_SEQUENCE=ATGAAGGTATTGGTGCTGGTCT
PRIMER_RIGHT_1_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_1=478,22
PRIMER_RIGHT_1=688,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=58.922
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=1.1864
PRIMER_LEFT_2_PENALTY=0.108206
PRIMER_RIGHT_2_PENALTY=1.078158
PRIMER_LEFT_2_SEQUENCE=AGGTATTGGTGCTGGTCTTGAT
PRIMER_RIGHT_2_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_2=482,22
PRIMER_RIGHT_2=688,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=58.922
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=1.2084
PRIMER_LEFT_3_PENALTY=0.130244
PRIMER_RIGHT_3_PENALTY=1.078158
PRIMER_LEFT_3_SEQUENCE=GGCTTAATGAAGGTATTGGTGC
PRIMER_RIGHT_3_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_3=472,22
PRIMER_RIGHT_3=688,22
PRIMER_LEFT_3_TM=59.870
PRIMER_RIGHT_3_TM=58.922
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=1.2104
PRIMER_LEFT_4_PENALTY=0.132262
PRIMER_RIGHT_4_PENALTY=1.078158
PRIMER_LEFT_4_SEQUENCE=TATTGGTGCTGGTCTTGATGAG
PRIMER_RIGHT_4_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_4=485,22
PRIMER_RIGHT_4=688,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=58.922
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=204
=
PRIMER_SEQUENCE_ID=SSR_02243 Ps_contig_mira-and-tgicl-ass_franssen_19593
SEQUENCE=CAACGCAGAGTACGGTCGGGGTTCACACAAATAAGAATAAGAACGAAGTTGCGAAACTAGGGTTTCCTTCTCCACACACACCAAATCGAATACCATCCAGAGACCAAAACCAGTCGAATTTGAAGCGCAATCATGCCGAAGAACAAGGGAAAGGGAGGAAAGAATCGAAAGAGGGGTAAGAACGAAGCCGACGATGAGAAACGCGAGCTTGTTTTCAAAGAAGATGGCCAAGAATATGCTCAAGTTCTCCGCATGTTAGGTAACGGTAGATGCGAAGCTATGTGCATCGACGGTACAAAGCGTCTCTGTCACATCCGTGGGAAGATGCACAAAAAGGTCTGGATTGCCGCCGGCGATATCATACTCGTTGGTCTCCGTGATTATCAGGATGATAAGGCTGATGTTATTCTCAAATACATGCCGGATGAGGCTCGTCTTCTTAAGGCGTATGGTGAGCTTCCTGATAATACTAGGCTTAATGAAGGTATTGGTGCTGGTCTTGATGAGGAAGATGATGGTGATGCTAATGATTATATCGAGTTTGAGGATGAGGATATTGATAAAATTTGAAAAAAAAGTTTTTTTTGTTTGTTTGAAGTTAAAAAGTTGAATTCTAATTATTATTACTATCGTTATATGTGATGATGATGAACATGATGATATGTAATGTTTGGGGTTTTATGCTGTACTCTATTATGGGATATCACAGTGATGTTGATATCGTGAATTGAATTTCAATGCACAAGAATAATTAATAGCTTGGTATTAAGAACTTAAAAAAAAAA
TARGET=640,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0782
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=1.078158
PRIMER_LEFT_SEQUENCE=GTATTGGTGCTGGTCTTGATGA
PRIMER_RIGHT_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT=484,22
PRIMER_RIGHT=688,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=58.922
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=1.1864
PRIMER_LEFT_1_PENALTY=0.108206
PRIMER_RIGHT_1_PENALTY=1.078158
PRIMER_LEFT_1_SEQUENCE=ATGAAGGTATTGGTGCTGGTCT
PRIMER_RIGHT_1_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_1=478,22
PRIMER_RIGHT_1=688,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=58.922
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=1.1864
PRIMER_LEFT_2_PENALTY=0.108206
PRIMER_RIGHT_2_PENALTY=1.078158
PRIMER_LEFT_2_SEQUENCE=AGGTATTGGTGCTGGTCTTGAT
PRIMER_RIGHT_2_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_2=482,22
PRIMER_RIGHT_2=688,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=58.922
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=1.2084
PRIMER_LEFT_3_PENALTY=0.130244
PRIMER_RIGHT_3_PENALTY=1.078158
PRIMER_LEFT_3_SEQUENCE=GGCTTAATGAAGGTATTGGTGC
PRIMER_RIGHT_3_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_3=472,22
PRIMER_RIGHT_3=688,22
PRIMER_LEFT_3_TM=59.870
PRIMER_RIGHT_3_TM=58.922
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=1.2104
PRIMER_LEFT_4_PENALTY=0.132262
PRIMER_RIGHT_4_PENALTY=1.078158
PRIMER_LEFT_4_SEQUENCE=TATTGGTGCTGGTCTTGATGAG
PRIMER_RIGHT_4_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_4=485,22
PRIMER_RIGHT_4=688,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=58.922
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=204
=
PRIMER_SEQUENCE_ID=SSR_02244 Ps_contig_mira-and-tgicl-ass_franssen_19594
SEQUENCE=GACAATAAGCAWWTTTGTMGAAGCATAAGAGGATTGAGTTGAAACACACCACTAAAAAACAAAGTAAAAAATCTGATAAAAAGTCTAACTTATGACATAAATTAAAGAGAACACATGAGAAATCCTTGAATAAAATACAAAAACAAAAAAGATAACAAAAGATAAAATGGAGGGATACAAATACACCCTTAAACATAACATACATAACCATAATCATAATCATAATCATATTCATGCTTATCTACAGTTTTGAGGACCCCATTATCATTTTGGCTTCTTTATCCATTCCAATTATTCCAAACACACAGGCCCATTCAGAACGTGGAATTGAAAGCAAGAGGACAAAAACTTCAACAGGGACCAGTAGACAACAACAACAACAACATCAACAACTCATAACAATAATTGAGGCCAAACATGTTGCATTCATTAAGCATTGCTCCTGAAGGATCCAAGAGCTTTAATTGCTGATCCTCTTAGAATGTATTGGTGGTAGATTGCAGCCACTGCAGCACCAATAAATGGTCCAACCCAATAAATCCACTGGTCATCCCAAGCTTTGTCGTTGTTGAAGATAACGGCGGGTCCGAAACTTCTGGCAGGGTTAATACCGGTTCCGGTGACGGGGATTGTTGCCAAGTGAACCATGAACACAGCAAATCCAATGGGAAGTGGTGCCAATACAGGAACATGAGAATCCCTAGCACTTCTTTTAGGATCAGTAGCAGAGAAAACAGTGTAGACTAGCACAAAGGTACCAATAATCTCAGCACCCAAAGCAGTGCCTTTACTGTAACCATCAGAAACAGTGTTAGCTCCTCCACCATACCTGTTGTAGTATGAACTTTGGAACCCCTTAGCAAGTCCAGCACCACAAATTGCACCCAAACATTGTTTGGTAGTACCTTTAA
TARGET=368,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02245 Ps_contig_mira-and-tgicl-ass_franssen_19595
SEQUENCE=ATCCTGTCGGGTTTAACCTTGTTGATGTTTTGGCTTGGGGTTCTATAGGTCATATTGTTGCTTATTATATCTTGGCCACTTCTAGCAATGGTTATGATCCTAGTTTCTTTGGTTAATTAAGATTACTATGTTGTTCGTTTATGTTGTTTTAATTTGTCTTTTCTTGTCCCTTTTATGTTTATTAAGATGATGATGATGATGATGATGATGTATAGTTTATTTGTATGAGATATTCAACAAGTTAAGTTCCTTGGATTAAAGCATTTTGATTTAGGTAAAGTAAAAGACTTCTCTGACT
TARGET=186,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02246 Ps_contig_mira-and-tgicl-ass_franssen_19597
SEQUENCE=TACGTCGGGGATCCTTCACTACCAACTATCACATTTCAATTCATCTTGAATCGAGTACAATGGATTCCAAAAATGCAATCCTCATCTTTGGTCTATTGGCCATGCTTCTTGTTATTTCCTCACAAGTGTCAGCTAGGGACTTAACTGAGGAGGTTGCTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGACACTATGGTCATGGTGGTGGTAGTTACCATGGTGGTGGCGGCAGTTACCACGGTGGACATGGTGGACATGGAGGACATGGTGGACATGGTGGTGTTTCCAACAATGAGGTTGTTGAAACTTCAAATGACGTAAATGATGCCAAATATGGACACTACGGTCATGGTGGTGGTAGTTACCATGGTGGTGGTGGCGGTTACCATGGTGGACATGGAGGACATGGAGGACACGGTGGACACGGTGGTGCTTCCGACAATGGCAACTGATTATATCATGATGCATGATGCACATTCAAAGTAATAAGTTTCATGAATGAATAGTGTTTGGTGTAAAATAAAAGAGGTACAAATAGTCATCATTATGTTGTGGTTATTATGTATTATTTTGTATATGGTGACGAGTTTTATTTCGAGTGAAAGTGATGAATGTGTTATTATTTGTAATATATCAAAATATATTATCGTTGTTTGTTAAWTTGATTTCCTTTTTAAGTTTTGTACTTTGCAWTAAAAACT
TARGET=374,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02247 Ps_contig_mira-and-tgicl-ass_franssen_19599
SEQUENCE=ACTTTAAAGAGAATANCATTATTTATATTAAGTTACATACAACATACANCACACATGGCTGGCAGATCACTAAAGTAATGAAAAACTGCCCATCACCAACGCACTCACCACCTAATAATAATAATCATTACAACAACACGTTACACTTACAACAACATCCTTTACATACAAAAGGGAAATAGTTAAAACAAGATAATGATCGAATTTCAAATTAAAAATAAAACCCCACAAAGTTGGTTTGGTTTAATGGTTTTGGCATCAACTATCACAAACCGTTGGATCATATCAACTTGACTTGAAAGGAATGGCTCTGATAACAACTGTGTGGTAAAGTGCTGCAAGTGCTGCACCAATGAATGGTCCAACCCAGAAAATCCATTGATCATCCCAACCAGATCTTGTGAAGATAATGCAGCACAGCTCAGCAGGTNATACNGTCAGTATGATAG
TARGET=113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2284
PRIMER_LEFT_PENALTY=0.095538
PRIMER_RIGHT_PENALTY=0.132857
PRIMER_LEFT_SEQUENCE=TGGCTGGCAGATCACTAAAGTA
PRIMER_RIGHT_SEQUENCE=TGTGATAGTTGATGCCAAAACC
PRIMER_LEFT=55,22
PRIMER_RIGHT=269,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=59.867
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=215
PRIMER_PAIR_PENALTY_1=0.2284
PRIMER_LEFT_1_PENALTY=0.095538
PRIMER_RIGHT_1_PENALTY=0.132857
PRIMER_LEFT_1_SEQUENCE=TGGCTGGCAGATCACTAAAGTA
PRIMER_RIGHT_1_SEQUENCE=GGTTTGTGATAGTTGATGCCAA
PRIMER_LEFT_1=55,22
PRIMER_RIGHT_1=273,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=0.2284
PRIMER_LEFT_2_PENALTY=0.095538
PRIMER_RIGHT_2_PENALTY=0.132857
PRIMER_LEFT_2_SEQUENCE=TGGCTGGCAGATCACTAAAGTA
PRIMER_RIGHT_2_SEQUENCE=GTGATAGTTGATGCCAAAACCA
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=268,22
PRIMER_LEFT_2_TM=59.904
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.2725
PRIMER_LEFT_3_PENALTY=0.095538
PRIMER_RIGHT_3_PENALTY=0.177008
PRIMER_LEFT_3_SEQUENCE=TGGCTGGCAGATCACTAAAGTA
PRIMER_RIGHT_3_SEQUENCE=TCAAGTTGATATGATCCAACGG
PRIMER_LEFT_3=55,22
PRIMER_RIGHT_3=293,22
PRIMER_LEFT_3_TM=59.904
PRIMER_RIGHT_3_TM=59.823
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=0.3371
PRIMER_LEFT_4_PENALTY=0.095538
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=TGGCTGGCAGATCACTAAAGTA
PRIMER_RIGHT_4_SEQUENCE=GAGCCATTCCTTTCAAGTCAAG
PRIMER_LEFT_4=55,22
PRIMER_RIGHT_4=310,22
PRIMER_LEFT_4_TM=59.904
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_02248 Ps_contig_mira-and-tgicl-ass_franssen_19600
SEQUENCE=TTACTTTGATTTTGATATTGATGGTACACAAAACTTACATTTGNAAACAAAGTTTGAAGACAGTAAGAATCAAAGATGGAAGTTCAAAGAGAAAGAGTTATTGGACCACTGAAACTCAAACTGCTAAGACAGAATCAGATGCAAAAATCACAATTGGACTTGCACCTGCTGAACTTGTTGTGGTTAATCCAAACATTAAAACTGCTGTTGGAAACGAAGTTGGTTATCGTTTGATTCCAGCAATTCCAGCTCATCCATTATTAACAGAGGATGATTATCCACAAATACGTGGTGCTTTTACAAACTATAATGTTTGGGTGACAGCATATAATAGAACTGAAAAATGGGCGGGTGGACTTTACGTTGATCATAGTCGCGGTGATGATACTTTGGCAGTTTGGACTAAACAGAATAGAGAGATTGTGAACAAGGACATAGTGATGTGGCATGTAGTTGGAATTCATCATGTTCCAGCACAGGAAGATTTTCCAATAATGCCACTATTGAGCACTTCATTTGAGCTGAGGCCAACCAATTTCTTTGAGAGGAATCCAGTTCTTAAAACACTTTCACCAAGAGATGTTGCATGGCCTGGTTGCTCCAATTAAGACTATCCAGCTTTTGTGTATGGAACTGATTTGCATTGGCCTTGTGGTCATATGAATAAATGTTTGTTTGTTTTGTTTGTTGCATGTGGAAAAAAATGTATCGGTCCCAACTATTCTAGTGCTTTCATATCTAGATTTTGCTATAATTGATCTGCAAGCAATAAAATGAATGGACTACACAGCAAAAGTG
TARGET=669,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3258
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.325733
PRIMER_LEFT_SEQUENCE=AATTCATCATGTTCCAGCACAG
PRIMER_RIGHT_SEQUENCE=AGCACTAGAATAGTTGGGACCG
PRIMER_LEFT=457,22
PRIMER_RIGHT=730,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=59.674
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.3301
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.325733
PRIMER_LEFT_1_SEQUENCE=ATGTTCCAGCACAGGAAGATTT
PRIMER_RIGHT_1_SEQUENCE=AGCACTAGAATAGTTGGGACCG
PRIMER_LEFT_1=465,22
PRIMER_RIGHT_1=730,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.674
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.4966
PRIMER_LEFT_2_PENALTY=0.170825
PRIMER_RIGHT_2_PENALTY=0.325733
PRIMER_LEFT_2_SEQUENCE=GGCATGTAGTTGGAATTCATCA
PRIMER_RIGHT_2_SEQUENCE=AGCACTAGAATAGTTGGGACCG
PRIMER_LEFT_2=444,22
PRIMER_RIGHT_2=730,22
PRIMER_LEFT_2_TM=59.829
PRIMER_RIGHT_2_TM=59.674
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.5026
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.502561
PRIMER_LEFT_3_SEQUENCE=AATTCATCATGTTCCAGCACAG
PRIMER_RIGHT_3_SEQUENCE=GCACTAGAATAGTTGGGACCGA
PRIMER_LEFT_3=457,22
PRIMER_RIGHT_3=729,22
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.503
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.5069
PRIMER_LEFT_4_PENALTY=0.004359
PRIMER_RIGHT_4_PENALTY=0.502561
PRIMER_LEFT_4_SEQUENCE=ATGTTCCAGCACAGGAAGATTT
PRIMER_RIGHT_4_SEQUENCE=GCACTAGAATAGTTGGGACCGA
PRIMER_LEFT_4=465,22
PRIMER_RIGHT_4=729,22
PRIMER_LEFT_4_TM=60.004
PRIMER_RIGHT_4_TM=60.503
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_02249 Ps_contig_mira-and-tgicl-ass_franssen_19602
SEQUENCE=CGTTCGTTTCCGGGTCTCCTTTTGGCTCCTCCTCCTCCGACCGCCACCACCAAACCCCGACCCACATCCAAGCANAGGTTCCCCTCNCCACCCCCGTTCAGCTAGGTGAAATCACCGAAGAAGAGCTCAAGGCCTACGATGGCAACGATCACGATAAGCCCTTGCTCATGGCCATTAAGGGCCAGATCTATGATGTCTCCCAGAGCAGGATGTTCTATGGACCCGGCGGTCCTTATGCTTTATTTGCTGGCAAGGATGCTAGTAGAGCTTTAGCAAAGATGTCTTTCGAAGATAAAGATCTGACCGGTGATATTTCCGGTCTTGGCCCATTTGAGCTCGACGCGTTACAAGACTGGGAATACAAGTTCATGGAGAAGTATGTTAAGGTGGGAACTATTAAAAAGGAAGTTCCAGTTACTGAATCCACCGGCGAACCATCAGAAACTACTCCCCGCGATGTTGATGCTGTTAATCCAACTGCTGCAGCTTAGTTAAAAGTGATAGAAACCACCTTACAAATACATTAARGDAAAAAAATAGGGAAAAAAGGAAAAGTTGTTGTAGGCSGACTAAATTTGTATGCATGTTAG
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02250 Ps_contig_mira-and-tgicl-ass_franssen_19613
SEQUENCE=TTASTCAAGGACAACTTTTTTATAATAGAGGATATATTTATTTGGCTTGCTTGATACAAAACCGAACTTTGAAAATTACAATTCATGCAAACAACTGCAGACACATGCATATTGAGGGCAGTGCCTTACAGCTGTGGCTAATACATCAAAAACACAAAATTAAAAAAAAAAAAGCAGCCCCCGAAACAATATTAATCAAACATTGACGAAATATATATGTGAAGAATCCCCTAGATCTTCTTTGTGCATGGCTTTCTTTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATTGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTGGTAGACAGGGTGAGGATGGGGATAGGTGTGCACTGGTGGTGGTGGAGGAAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGAGGAGGAGAGTGGTAGACTGGGTGTGGGTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATATTTCAGATGGGAAACTGAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCATTAGGGACCTCATTGTTTTTCTTATGAGTGTATTTTGGTTATCCCCGACGTACTCTGTCGTTGACTACCACTGTCTT
TARGET=406,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02251 Ps_contig_mira-and-tgicl-ass_franssen_19613
SEQUENCE=TTASTCAAGGACAACTTTTTTATAATAGAGGATATATTTATTTGGCTTGCTTGATACAAAACCGAACTTTGAAAATTACAATTCATGCAAACAACTGCAGACACATGCATATTGAGGGCAGTGCCTTACAGCTGTGGCTAATACATCAAAAACACAAAATTAAAAAAAAAAAAGCAGCCCCCGAAACAATATTAATCAAACATTGACGAAATATATATGTGAAGAATCCCCTAGATCTTCTTTGTGCATGGCTTTCTTTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATTGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTGGTAGACAGGGTGAGGATGGGGATAGGTGTGCACTGGTGGTGGTGGAGGAAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGAGGAGGAGAGTGGTAGACTGGGTGTGGGTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATATTTCAGATGGGAAACTGAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCATTAGGGACCTCATTGTTTTTCTTATGAGTGTATTTTGGTTATCCCCGACGTACTCTGTCGTTGACTACCACTGTCTT
TARGET=618,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02252 Ps_contig_mira-and-tgicl-ass_franssen_19618
SEQUENCE=GGTGGATAWCRRGGAAGTTTTGGTTCTCAAATTCAATCTAAACAAGAAAGCAGATAACAGAAAAAAAGGTGTGAGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGCAGCAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTGCTCTGCTTTGCCTTATCTCCCTCCTCCTCGCCTCTCCACCACCACTCCTTCTTCTTCCCCTTCCCTAACTATTTTTCAGAGTCGCGGTAAGTTTTCTCTTCATCGGACTAGAGCTTCTTCTTCTGATGATACATCTACTTCTCTCGATACCAATGAGTTGTTTACCGATTTGAAGGAGAAGGCAAGAAGCTTATATAATTTGTTGAATTCAATAATTTTAGTTTCATGTTGATTTATCTTCTGTCAAATCCATAATGTTTCATTTTCTATTCTAAATAAGAAACACAAACTGACATGACACTAGCACATTGGAGTCATGACGATACCAGATACACCTTTAATTTGGAGTGTTGGCGCTAACCCTATTGTCTTTTATAGTTTTACTAAACAAAGTAGAAATTATATCTTCCTGCATTTTT
TARGET=73,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02253 Ps_contig_mira-and-tgicl-ass_franssen_19628
SEQUENCE=ANAAGAAGATTGTGGGATGACCAAAAACTTYWAMCTAAMTMCYTAAYTTWGTCATCAGAGTCATCAACTAATGTGGGTTAGGGCGTAAACATATGGTTTATGATTATTGTAATGATGCTTCTAGGTTTCCTGTTACTCCTCTTGAGTGTCTTCATCATCGTCATAGATTATTCTAATCATGTCAAACTTGTGTATTATTATTATGGCTGGAAATGGTGAATGTTGGTGTAAGAAATGTTCCACTTGTGTCATATTATTGTATGATATTATTGGCAAACTATATATAATGTGGAGAGAAAGTGTTAAATAAGGTGATGTCAAACTTGTGTATTATTATGGGTGGAAATGGTGAATATTGGTGTAAGAAAGTTGGGGAATATGTTCCACTTATGTCATATTATTGTATGATATTATTGGCAAACTATATATAATGTGAATTACTTGGTATTTATAAAMKTT
TARGET=193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02254 Ps_contig_mira-and-tgicl-ass_franssen_19630
SEQUENCE=GTCGAGGGCAGCGACAATGGTGGCAAAGCGGCACTTGACGACAATGGTGGAGGTGGTTACCATAATGGTGGAGGTGGTTACAATGGCGGTGGAGGTTACCATAATGGTGGTGGTGGTTACCATGGTGGAGGTGGACACGGTGGACATGGTGGTGCTTCCAACAACGGTAACTGATAATATCAACATTCTCAGTAATAAATATCATGAATTAATAATGTTGGATGTTGAAAAAAAGAGAGGTATAGATAGACATGAATCTTTTATGGTTATGTATTATCTATCTTGCAACTCTTTAGTATAGTTATTTCTCAACTCTATAAATCACTATGTATATGTGATGGATGATGAGTTTTATTTTGTTACCAAAGTGTTGAATGTTCTATCATGTGTAATATATCAAATTTCATAAACTTTAGTAAGT
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7650
PRIMER_LEFT_PENALTY=0.352395
PRIMER_RIGHT_PENALTY=0.412596
PRIMER_LEFT_SEQUENCE=CCATAATGGTGGAGGTGGTTAC
PRIMER_RIGHT_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_LEFT=59,22
PRIMER_RIGHT=174,22
PRIMER_LEFT_TM=60.352
PRIMER_RIGHT_TM=60.413
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=0.7650
PRIMER_LEFT_1_PENALTY=0.352395
PRIMER_RIGHT_1_PENALTY=0.412596
PRIMER_LEFT_1_SEQUENCE=TGGTGGAGGTGGTTACCATAAT
PRIMER_RIGHT_1_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_LEFT_1=44,22
PRIMER_RIGHT_1=174,22
PRIMER_LEFT_1_TM=60.352
PRIMER_RIGHT_1_TM=60.413
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.7650
PRIMER_LEFT_2_PENALTY=0.352395
PRIMER_RIGHT_2_PENALTY=0.412596
PRIMER_LEFT_2_SEQUENCE=AATGGTGGAGGTGGTTACCATA
PRIMER_RIGHT_2_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=174,22
PRIMER_LEFT_2_TM=60.352
PRIMER_RIGHT_2_TM=60.413
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=133
PRIMER_PAIR_PENALTY_3=0.7650
PRIMER_LEFT_3_PENALTY=0.352395
PRIMER_RIGHT_3_PENALTY=0.412596
PRIMER_LEFT_3_SEQUENCE=ATGGTGGAGGTGGTTACCATAA
PRIMER_RIGHT_3_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_LEFT_3=43,22
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=60.352
PRIMER_RIGHT_3_TM=60.413
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=0.8034
PRIMER_LEFT_4_PENALTY=0.390771
PRIMER_RIGHT_4_PENALTY=0.412596
PRIMER_LEFT_4_SEQUENCE=CATAATGGTGGAGGTGGTTACA
PRIMER_RIGHT_4_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_LEFT_4=60,22
PRIMER_RIGHT_4=174,22
PRIMER_LEFT_4_TM=59.609
PRIMER_RIGHT_4_TM=60.413
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_02255 Ps_contig_mira-and-tgicl-ass_franssen_19631
SEQUENCE=TATTTTATTAATTAAGTTACACAATACAACCANACANCACACATGGCGTGGCCAGATCCAACTAAAGTAATGAAAAATAGCCCANCACCCAACGCACTCACCACCTAATAATAATAATCATTACAACAACACGTTACACTTACAACAACATCCTTTACAAAAAGGGAAATAGTTAAAACAAGATAATGATCGAATTTCAAATTAAAAATAAAACCCCACAAAGTTGGTTTGGTTTAATGGTTTTGGCATCAACTATCACAAACCGTTGGATCATATCAACTTGACTTGAAAGGAGTGGCTCTGATAACAACTGTGTGGTAAAGTGCTGCAAGTGCTGCACCAATGAATGGTCCAACCCAGAAATCCATTGATCACCCCAACAAGATCTTTGTGAAGATATGCAGCACAGCTCTAGCAGGGTT
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3378
PRIMER_LEFT_PENALTY=1.204914
PRIMER_RIGHT_PENALTY=0.132857
PRIMER_LEFT_SEQUENCE=GTAATGAAAAATAGCCCANCACC
PRIMER_RIGHT_SEQUENCE=TGTGATAGTTGATGCCAAAACC
PRIMER_LEFT=66,23
PRIMER_RIGHT=259,22
PRIMER_LEFT_TM=59.795
PRIMER_RIGHT_TM=59.867
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.75
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5750
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=1.3378
PRIMER_LEFT_1_PENALTY=1.204914
PRIMER_RIGHT_1_PENALTY=0.132857
PRIMER_LEFT_1_SEQUENCE=GTAATGAAAAATAGCCCANCACC
PRIMER_RIGHT_1_SEQUENCE=GTGATAGTTGATGCCAAAACCA
PRIMER_LEFT_1=66,23
PRIMER_RIGHT_1=258,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.75
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5750
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.75
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=1.3378
PRIMER_LEFT_2_PENALTY=1.204914
PRIMER_RIGHT_2_PENALTY=0.132857
PRIMER_LEFT_2_SEQUENCE=GTAATGAAAAATAGCCCANCACC
PRIMER_RIGHT_2_SEQUENCE=GGTTTGTGATAGTTGATGCCAA
PRIMER_LEFT_2=66,23
PRIMER_RIGHT_2=263,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.75
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5750
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.75
PRIMER_PAIR_2_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=1.3693
PRIMER_LEFT_3_PENALTY=1.236418
PRIMER_RIGHT_3_PENALTY=0.132857
PRIMER_LEFT_3_SEQUENCE=TGGCCAGATCCAACTAAAGTAA
PRIMER_RIGHT_3_SEQUENCE=TGTGATAGTTGATGCCAAAACC
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=58.764
PRIMER_RIGHT_3_TM=59.867
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=1.3693
PRIMER_LEFT_4_PENALTY=1.236418
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_LEFT_4_SEQUENCE=TGGCCAGATCCAACTAAAGTAA
PRIMER_RIGHT_4_SEQUENCE=GGTTTGTGATAGTTGATGCCAA
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=263,22
PRIMER_LEFT_4_TM=58.764
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_02256 Ps_contig_mira-and-tgicl-ass_franssen_19639
SEQUENCE=AATAGAGTTGAGGAATAAAGAAATCCTCATTTTCTAAACCTTACTAGTTTGTATTTTTGGTAACTATTTTGGTCTTGTCCGGATTTTTGTATGACTAGGATGATCTGGCTTTTTGTGCATCTGTAGATAATAAATAAATAATAAGAATCAGAATAATGCTACTGAGAAATATTATCCTTAAACAGATGATTAGTGAGTTTCTTTGTTTATTTTTTTTAGTTTCTGAAATGCCAATGAATTTCAATAAATGTTCCTGTTTATATAGTATGAGAATAATAACTTCTCAAAACAATGTTCACTATAAGGAGTTTGTTTTATTTATAATATGTTATGTGTTTGATCCATTTAATGTTTTTTTAGNGATTGGTGAAGATGAAC
TARGET=129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02257 Ps_contig_mira-and-tgicl-ass_franssen_19641
SEQUENCE=GAACSACCATGTAAAGGTTCCGTATTGATTCCTRCTTCCTTCCATTATAAATAGACACCACTTTCTTGCTATGTCGGGGATCCTTCACTACCAAATATCACATTTCAATTCTTCTTGAGTTCAGTACAATGGATTCCAGAAAAGCAATGCTCATTCTTGGCCTATTGGCCATGGTTCTTCTTATTTCATCAGAGGTGTCAGCTAGGGAATTAACTGAGGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCTAAATATGGAGGTTATGGACGTGGTGGTGGTTACCATAATGGAGGTGGTTACCACAATGGTGGAGGAGGATACAATGGCGGTGGCGGATACCACAATGGTGGAGGAGGATACAATGGCGGTGGCGGTTACCACAATGGTGGAGGTGGTTACCATAATGGTGGAGGTGGTTACAATGGCGGTGGAGGTTACCATAATGGTGGTGGTGGTTACCATGGTGGAGGTGGACACGGTGGACATGGTGGTGCTTCCAACAACGGTAACTGATAATATCAACATTCTCAGTAATAAATATCATGAATTAATAATGTTGGATGTTAGAAAAAAAAGAGAGGTATAGATAGACATGAATTCTTTTATGGTTATGTTATTATCTTATTCTTGACCTAACTTCTTTTAAGTTAWTTTAA
TARGET=449,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02258 Ps_contig_mira-and-tgicl-ass_franssen_19646
SEQUENCE=GTACGAAGTTACCGGTTGGTCCGACTGGGAAGTCGAACGACTCCTCGGAGGTTGTTTCGTTTKTGCTGGRRGCACACAGTGTTGGGTTAGAACTCATTGCACAAAACTGGTGCACCGTTTATATCCAGAAGTTGATCCAGCTTTGAATCCAGATCACATTCCACACATGCTAAAGAAGTGTCCTGATTCAATCCCTGACCCTAAGGCAGTACAGTACGTGAGAAATGACCGTGGTACCCCCATGATTCTTGATAACAATTACTATAGAAATATTCTCGACAACAAGGGTCTTTTAATAGTGGATCATCAACTAGCACATGACAAAAGGACAAAACCTTATGTGAAGAAAATGGCCAAAAGTCAAGACTATTTCTTCAAGGAGTTTTCTAAAGCTATTACATTGCTTTCTGAGAATAATCCTCTAACCGGTACTAAAGGTGAGATTAGAAAACAGTGCAGTGTTGCTAACAAACAACACCATGATGAGCCTTGAATGAAAAATTCATGGTGGATGAAGAAGAAGTCATGTGTAAGATATTATTATGCAGAAATAAAAGTGTTCATGGAGATTCAAGAACTTAGATGCTTTTAATTTTAATGGTAACTTTTGTTTTTTATTTATTTAATATTAGTAGTATGGTAGCTCAATGTGGAGCATGTGGATGATGTGATGTATGGTATTCATGTGGTGCATGATGGCAGTKGTGTGAACTTAAG
TARGET=614,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02259 Ps_contig_mira-and-tgicl-ass_franssen_19648
SEQUENCE=AATACACGTAGAAGTACTCTAACCAAANCTANAATAAACTTAAATAAATACATAAACGAATAACCCACNCTAAAACAACTACTCCCTCCCACGTTTCCTACCGCCACCCGCCTCCTCCACCGAGAATAACCGACCTCCTCCACCACTTCCTCCAYACCMMCGATCCCGGCTTCCGCCATAACCACCACYACCATATCCTCCTCCTCCACTACCGTATCCTCCTCCTCCACCTTCACGACGGCCACCATAGCCTCCACCTCCACCATATCCACCACCACCACGTCCACCTCCATATCCACCGCCACCACCACGGCCGCCTCCACCGCTACCACGGCTCTGAGCTTCGTTTACTGTGATGTTACGTCCATCCATGTCCTGACCGTTCATCCCTTCGATCGCATCTCTCATGGATTGCTCCGTGGCAAAGGTCACAAATCCAAAACCTCTTGACCTTCCAGTCTCACGATCGTTAATGATCTTCGAGTCGACGATTTCACCGTATTGAGAGAAAGCTTTCTCTAGGGCATCGCTGTCAGTGGCCCATGCTAGACCACCAACGAAGCACCGGTACTCAACATCTGAGGAAGCCATGGAAGAAACACTGAAATTGGGACTGAGATGGTGTAAAGGCGAAAGATTGCTTSAAGATCCCCGCGTACT
TARGET=196,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02260 Ps_contig_mira-and-tgicl-ass_franssen_19648
SEQUENCE=AATACACGTAGAAGTACTCTAACCAAANCTANAATAAACTTAAATAAATACATAAACGAATAACCCACNCTAAAACAACTACTCCCTCCCACGTTTCCTACCGCCACCCGCCTCCTCCACCGAGAATAACCGACCTCCTCCACCACTTCCTCCAYACCMMCGATCCCGGCTTCCGCCATAACCACCACYACCATATCCTCCTCCTCCACTACCGTATCCTCCTCCTCCACCTTCACGACGGCCACCATAGCCTCCACCTCCACCATATCCACCACCACCACGTCCACCTCCATATCCACCGCCACCACCACGGCCGCCTCCACCGCTACCACGGCTCTGAGCTTCGTTTACTGTGATGTTACGTCCATCCATGTCCTGACCGTTCATCCCTTCGATCGCATCTCTCATGGATTGCTCCGTGGCAAAGGTCACAAATCCAAAACCTCTTGACCTTCCAGTCTCACGATCGTTAATGATCTTCGAGTCGACGATTTCACCGTATTGAGAGAAAGCTTTCTCTAGGGCATCGCTGTCAGTGGCCCATGCTAGACCACCAACGAAGCACCGGTACTCAACATCTGAGGAAGCCATGGAAGAAACACTGAAATTGGGACTGAGATGGTGTAAAGGCGAAAGATTGCTTSAAGATCCCCGCGTACT
TARGET=217,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02261 Ps_contig_mira-and-tgicl-ass_franssen_19648
SEQUENCE=AATACACGTAGAAGTACTCTAACCAAANCTANAATAAACTTAAATAAATACATAAACGAATAACCCACNCTAAAACAACTACTCCCTCCCACGTTTCCTACCGCCACCCGCCTCCTCCACCGAGAATAACCGACCTCCTCCACCACTTCCTCCAYACCMMCGATCCCGGCTTCCGCCATAACCACCACYACCATATCCTCCTCCTCCACTACCGTATCCTCCTCCTCCACCTTCACGACGGCCACCATAGCCTCCACCTCCACCATATCCACCACCACCACGTCCACCTCCATATCCACCGCCACCACCACGGCCGCCTCCACCGCTACCACGGCTCTGAGCTTCGTTTACTGTGATGTTACGTCCATCCATGTCCTGACCGTTCATCCCTTCGATCGCATCTCTCATGGATTGCTCCGTGGCAAAGGTCACAAATCCAAAACCTCTTGACCTTCCAGTCTCACGATCGTTAATGATCTTCGAGTCGACGATTTCACCGTATTGAGAGAAAGCTTTCTCTAGGGCATCGCTGTCAGTGGCCCATGCTAGACCACCAACGAAGCACCGGTACTCAACATCTGAGGAAGCCATGGAAGAAACACTGAAATTGGGACTGAGATGGTGTAAAGGCGAAAGATTGCTTSAAGATCCCCGCGTACT
TARGET=269,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02262 Ps_contig_mira-and-tgicl-ass_franssen_19668
SEQUENCE=CTTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGTTCTTTGATGGGGTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGTGTATTATATTGCAATGAAATTGAAAAGATTGTTATGCTTTTGATAATTGTATCCTTATCAATGGTC
TARGET=115,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5811
PRIMER_LEFT_PENALTY=0.189529
PRIMER_RIGHT_PENALTY=1.391547
PRIMER_LEFT_SEQUENCE=CTTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_SEQUENCE=GCAATATAATACACGATGGCCG
PRIMER_LEFT=0,22
PRIMER_RIGHT=151,22
PRIMER_LEFT_TM=59.810
PRIMER_RIGHT_TM=61.392
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=1.8462
PRIMER_LEFT_1_PENALTY=0.454655
PRIMER_RIGHT_1_PENALTY=1.391547
PRIMER_LEFT_1_SEQUENCE=CTCCCAATTCTTGGTTCTTTGA
PRIMER_RIGHT_1_SEQUENCE=GCAATATAATACACGATGGCCG
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=151,22
PRIMER_LEFT_1_TM=60.455
PRIMER_RIGHT_1_TM=61.392
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=1.8569
PRIMER_LEFT_2_PENALTY=0.465395
PRIMER_RIGHT_2_PENALTY=1.391547
PRIMER_LEFT_2_SEQUENCE=AGCTCCCAATTCTTGGTTCTTT
PRIMER_RIGHT_2_SEQUENCE=GCAATATAATACACGATGGCCG
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=151,22
PRIMER_LEFT_2_TM=60.465
PRIMER_RIGHT_2_TM=61.392
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=115
PRIMER_PAIR_PENALTY_3=2.0523
PRIMER_LEFT_3_PENALTY=0.660713
PRIMER_RIGHT_3_PENALTY=1.391547
PRIMER_LEFT_3_SEQUENCE=TTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_3_SEQUENCE=GCAATATAATACACGATGGCCG
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=151,22
PRIMER_LEFT_3_TM=60.661
PRIMER_RIGHT_3_TM=61.392
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=12.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=2.0847
PRIMER_LEFT_4_PENALTY=0.693167
PRIMER_RIGHT_4_PENALTY=1.391547
PRIMER_LEFT_4_SEQUENCE=CCCAATTCTTGGTTCTTTGATG
PRIMER_RIGHT_4_SEQUENCE=GCAATATAATACACGATGGCCG
PRIMER_LEFT_4=41,22
PRIMER_RIGHT_4=151,22
PRIMER_LEFT_4_TM=60.693
PRIMER_RIGHT_4_TM=61.392
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=12.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=111
=
PRIMER_SEQUENCE_ID=SSR_02263 Ps_contig_mira-and-tgicl-ass_franssen_19681
SEQUENCE=GAAACATCAAATGACGTAAATGATGCCAAATATGGACACTACGGTCATGGTGGTGGTAGTTACCATGGTGGTGGTGGCGGTTACCACGGTGGACATGGAGGACATGGTGGACATGGTGGACACGGTGGTGCTTCCGACAATGGCAACTGATAATACCATGATACATGATGCACATTCAAAGTAATAAGTTTCATGAATGAATAGTGTTTGGTGTAAAATAAAAGAGGTAACAAATAGTCATCATTATGTTG
TARGET=66,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4002
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=0.146683
PRIMER_LEFT_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_SEQUENCE=TATTATCAGTTGCCATTGTCGG
PRIMER_LEFT=25,22
PRIMER_RIGHT=154,22
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=59.853
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=0.7813
PRIMER_LEFT_1_PENALTY=0.634599
PRIMER_RIGHT_1_PENALTY=0.146683
PRIMER_LEFT_1_SEQUENCE=GATGCCAAATATGGACACTACG
PRIMER_RIGHT_1_SEQUENCE=TATTATCAGTTGCCATTGTCGG
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=154,22
PRIMER_LEFT_1_TM=59.365
PRIMER_RIGHT_1_TM=59.853
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=1.1227
PRIMER_LEFT_2_PENALTY=0.976051
PRIMER_RIGHT_2_PENALTY=0.146683
PRIMER_LEFT_2_SEQUENCE=ATGCCAAATATGGACACTACGG
PRIMER_RIGHT_2_SEQUENCE=TATTATCAGTTGCCATTGTCGG
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=60.976
PRIMER_RIGHT_2_TM=59.853
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=133
PRIMER_PAIR_PENALTY_3=1.1609
PRIMER_LEFT_3_PENALTY=1.014220
PRIMER_RIGHT_3_PENALTY=0.146683
PRIMER_LEFT_3_SEQUENCE=AAATATGGACACTACGGTCATGG
PRIMER_RIGHT_3_SEQUENCE=TATTATCAGTTGCCATTGTCGG
PRIMER_LEFT_3=27,23
PRIMER_RIGHT_3=154,22
PRIMER_LEFT_3_TM=60.014
PRIMER_RIGHT_3_TM=59.853
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=128
PRIMER_PAIR_PENALTY_4=1.2402
PRIMER_LEFT_4_PENALTY=1.093501
PRIMER_RIGHT_4_PENALTY=0.146683
PRIMER_LEFT_4_SEQUENCE=CTACGGTCATGGTGGTGGTAGT
PRIMER_RIGHT_4_SEQUENCE=TATTATCAGTTGCCATTGTCGG
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=154,22
PRIMER_LEFT_4_TM=61.094
PRIMER_RIGHT_4_TM=59.853
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=117
=
PRIMER_SEQUENCE_ID=SSR_02264 Ps_contig_mira-and-tgicl-ass_franssen_19685
SEQUENCE=AAACCGAACTGCTCTTGATGCTGCAGGAAACAACAACTAGCTGCCATTGGAAAGGGGGTTTGGTCTATTGGTTCTGCCGCCCTTGTGTCTTTGGCCTTTTGGTGCCTTTGTGAGCCGTGCTGGTGTTACAACCGTCGATGTCCTGACTCCCAAGGTTTTCATCGGTCTGATTGTGGGCGCCATGCTCCCTTACTGGTTTTCTGCCATGACCATGAAGAGTGTCGGAAGTGCAGCATTGAAGATGGTTGAGGAAGTTCGCAGGCAATTCAACACGATTCCCGGATTGATGGAGGGAACCGCGAAACCTGACTATGCCACATGTGTTACAATCTCCACCGACGCTTCCATCAAAGAAATGATCCCACCTGGTGCCCTTGTTATGCTAACACCCCTTATTGTTGGGATCTTTTTTGGTGTTGAAACACTTTCTGGTGTCCTTGCCGGATCATTGGTTTCTGGTGTACAGATTGCCATCTCTGCATCCAACACTGGCGGTGCTTGGGATAATGCCAAGAAGTACATCGAGGCTGGTGCCTCAGAGCATGCAAGAAGCCTAGGTCCAAAAGGGTCAGACCCACACAAGGCAGCAGTTATTGGAGACACCATTGGAGACCCATTGAAGGATACATCTGGACCTTCACTTAACATCCTTATCAAGCTGATGGCAGTTGAGTCACTGGTGTTTGCTCCTTTCTTTGCAACCCACGGTGGTCTCCTCTTCAAGTTTTGGTAATAGAAGAGCATCGTCCATCCCACCCACCAACCAACCAACCATGGCGTCTCTCTTTTTTGTTAGTTTGAYGGCAACTTGTTTTTNTTTTATATTNTCTTATTATACTCCCCCTATCCCTNTTGTA
TARGET=759,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02265 Ps_contig_mira-and-tgicl-ass_franssen_19688
SEQUENCE=TTTACTGTTTCTTGATAMAAAACRAAGGGGTAAAAACGTAGAGACACAATTCATGGGTCTCCAAAGTGACAAGATGATGAATCACACAACAAAACTTTACATTATTTTCAAGTGAATCGCCAACATAGTCCTGAAATTGTAGATGTCGTAAAATTTAGTTCCTGAAATAGACAAAATTCTAAAATGATCCTTGTAACCGGAAGTCCTTGATCACACGTAATTTTGGGTGGGACATTTTAGTTCCCTTGAAATTGATTCTTTAGGAGCTAATGCGATCAACAATTTTCAATTTTTTGGAAATCATTTTATAATTTTGTTAATTTCAAGGATCGAAATTCTTGATTCTTAGACTTCGAGGACTAAATTGATGATTCACTATGTTATTTCCATACCATAAGATTAAGGGGTGACTACATCAAGTTGGTGGATACAATTGATATCTACAAAATGATTTGAGTTTTTTTATTAGTAGGAATCAAATTCTGTAGTTCGCAATTTACAACACACACACACTGCGCACCAACAACAAAACAATTGAGCTGATTCACATGAGAGAAGAGACCATGTTCTACATGAAACCCTAATCTCTTTATTCCCGATTGAAGAAAATAAACCACACCAAACAAAGTATAGTGGCTTTTTGTGAAGAGAAATAAACTCACACACATGATGACACACAAGAGCATGATGATTGATTAGGAGTGACTAGTATATGGAGCAACACACCTTGCCCATTTGAACAATTTCAAGTGATGCATCTTCTTGATTTTGTTTGGAGAATTGCCACCAAACTCTGATAATGATTCATTCCTGTTCAACATCTCCCACATCACACGAATATCCTCATACTCACAAGCCCTCACATCATTTCTTAGCTTCTTTAGCCCAGTTTTACGAATTCCAAATCGTGTGGCGATGGTAAGCCAAAGCCTTCGAACACGATAGATGATGTTCTTCTGCCACCACTCCACCTGCCGAGAAAAATTGAGTTTCTCCATTGAATAGAGCCTCGGTAGGTGAAGATGAAGATACACACTCTCACCACGACTTCACAATACCGATGATATCCCCGAC
TARGET=502,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02266 Ps_contig_mira-and-tgicl-ass_franssen_19691
SEQUENCE=AATAGTCCTCCCCTAAAACTACAAAGGAATGCCAGATCACATGGAGAGCCAATCATGAAATGGGTGWKRWTGGKGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGAGGAGGAGAGTGGTAGACTGGGTGTGGGTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATATTTCAGATGGGAAACTGAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCATTAGGGACCTCATTGTTTTTCTTATGAGTGTATTTTGGTTCTCGAGTACT
TARGET=88,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02267 Ps_contig_mira-and-tgicl-ass_franssen_19691
SEQUENCE=AATAGTCCTCCCCTAAAACTACAAAGGAATGCCAGATCACATGGAGAGCCAATCATGAAATGGGTGWKRWTGGKGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGAGGAGGAGAGTGGTAGACTGGGTGTGGGTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATATTTCAGATGGGAAACTGAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCATTAGGGACCTCATTGTTTTTCTTATGAGTGTATTTTGGTTCTCGAGTACT
TARGET=299,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02268 Ps_contig_mira-and-tgicl-ass_franssen_19696
SEQUENCE=ACAACAACAACACAACCAATACTTTAWTTACTAACTAGATATGGGTGGCGKAGTCATCAGCAGCTGGTGGAGCTTGAGATATGGCAGATAATACAACAAAATAGTACCAAATAAGATTGTGATTATTGCAGTGAAGTTCACAAGTAAAGCCTCAGAACCATATTTTGCAACTCCTGAACTCTCCAAGAATGTCAGCTTCTCCAGAAACCCAAGAGAAGCAGTCGCAAGAGCCAAAATATACACAAACAGCCCAAATAACACATGCCATGGAACTGACTCACGTCTTATCTCTGAACTCCCACCAGGGTAGAAAAATACCACAAACCCAAATATCCACTGAATGCCATAAAAGGAAATAACCCCAATTCCAAGCCATGAATGTAAGCTGTACAGATTGGCAATCCCACTTTCATTGTGATTCTTGAACGCAGCACAAATTCCAATTATTCCAAGTATTAACGCAATCGCGTGGAGAACAAGGTGTATCAATTTCTTCACTTCCTTCTTCAAGGGAAGAGATTTGTAACTTATAATCGCTTCACCTCCAACTATTATTAAGCCAATAAGCATAAGAACAGGATGAAGATTGAAGATGAGAGCCTTGTTGGTAGAACTCCAAGCCAAACCACCTCTGAAATGGAAGTTCCAGACCAACACCAACACGGCGGCGACAACACCGAGAAGGTGAACAACGTACGTGAACGGAACGGCGGGTACACCCAACGCCATCTTCAACGGTTATTGGTGTTGTTGTTGTTGGGAGAAGATTCTGGGAAGATAAAGGCGCACTCTGACACGAGTTTCGTCGGTGTAGTTGTTAAGATATACGGTGTGTGGAAGTACAGGTCATCTGACTACCCTTCTAGCCCAACCAACCCCCGACCCCCCGTACG
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02269 Ps_contig_mira-and-tgicl-ass_franssen_19696
SEQUENCE=ACAACAACAACACAACCAATACTTTAWTTACTAACTAGATATGGGTGGCGKAGTCATCAGCAGCTGGTGGAGCTTGAGATATGGCAGATAATACAACAAAATAGTACCAAATAAGATTGTGATTATTGCAGTGAAGTTCACAAGTAAAGCCTCAGAACCATATTTTGCAACTCCTGAACTCTCCAAGAATGTCAGCTTCTCCAGAAACCCAAGAGAAGCAGTCGCAAGAGCCAAAATATACACAAACAGCCCAAATAACACATGCCATGGAACTGACTCACGTCTTATCTCTGAACTCCCACCAGGGTAGAAAAATACCACAAACCCAAATATCCACTGAATGCCATAAAAGGAAATAACCCCAATTCCAAGCCATGAATGTAAGCTGTACAGATTGGCAATCCCACTTTCATTGTGATTCTTGAACGCAGCACAAATTCCAATTATTCCAAGTATTAACGCAATCGCGTGGAGAACAAGGTGTATCAATTTCTTCACTTCCTTCTTCAAGGGAAGAGATTTGTAACTTATAATCGCTTCACCTCCAACTATTATTAAGCCAATAAGCATAAGAACAGGATGAAGATTGAAGATGAGAGCCTTGTTGGTAGAACTCCAAGCCAAACCACCTCTGAAATGGAAGTTCCAGACCAACACCAACACGGCGGCGACAACACCGAGAAGGTGAACAACGTACGTGAACGGAACGGCGGGTACACCCAACGCCATCTTCAACGGTTATTGGTGTTGTTGTTGTTGGGAGAAGATTCTGGGAAGATAAAGGCGCACTCTGACACGAGTTTCGTCGGTGTAGTTGTTAAGATATACGGTGTGTGGAAGTACAGGTCATCTGACTACCCTTCTAGCCCAACCAACCCCCGACCCCCCGTACG
TARGET=746,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02270 Ps_contig_mira-and-tgicl-ass_franssen_19697
SEQUENCE=CGGGGGAGCTTTTTCTTCACATCNTAAAACCGGACAAAAGAAGTAGTCGGGGAGAGTAACAATAATTAACAATGTCGACCTTAACTGTTCCTCATCCTCTTCCTCCTGTCTCCGATGACGTTGAGCAGCTCCGCAAAGCCTTTTCAGGTTGGGGGACCAATGAAGATTTGATTATATCGATTTTGGGTCATAGGAATGGTAGTCAGAGGAAGGCTATCAGAGAAGGTTATGCTCAGACTTATGGAGAAGATCTCCTTAAGGCCTTGGACAAAGAGCTTACCAGTGACTTTGAGAGGCTGGTTCATCTTTGGGCACTTGAATCCGCGGAACGCGATGCTTTTCTTGCAAACGAAGCAACCAAAAAATGGACATCAAGCAATCAAGTTCTGGTGGAAATTGCTTGCACTAGATCTTCTGATCAATTGTTTTCTGCAAAGAAGGCTTATCATGTTCTTTATAAGAAGTCTCTTGAGGAAGATGTTGCTCATCACACAACTGGAGACTACCGTAAGCTCCTATTACCTCTGGTTAGTTGTCACCGCTATGAAGGAGATGAAGTGAACTTGACTATAGCAAAAGCGGAGGCAAAGATACTTCACGAAAAGATTTCAAAAAAGGCCTATAACGACGACGATCTCATTAGGATTCTGGCCACAAGGAGCAAAGCACAAGTTAATGCTACTTTGAATCACTACAAAGATGCATTTGGAAAAGATATAAACAAGGATCTGAAAGAAGATCCAAAAGACGAGTTTCTGTCATTACTGAGATCAACTGTCAAGTGCTTGACTCGTCCCGAGAAGTACTTTGCAAAGATAATTCGTCTGTCGATCAATAAGCGAGGAACGGATGAAGGGGCTCTTACAAGAGTTGTGGCAACAAGAGCTGAAATTGACTTGAAAATTGTGGGTGATGAGTATCAAAGGAGGGACAGTGTCCCTCTTGATCGTGCCATTGTTAAGGACACCACTGGTGACTATGAGAAAATGCTTTTGGCAATCTTAGGACATGATGATGCTTGAGATATATGTTGGTATGTTTGTGTATGTGTGTGTGTTTTCCTTTTTGAGAGAGGTACTATGTGGTTTTATAAGCTGTGTTTTCAGCATGGTTGTTTTTCCTTTGTGTTATTGTGATTTTGGGAGTATAATTTGAATTGTACTTCAATAATGTGTGTGATTAGCTTTTGTAATCACTATAGTGACTTTATGGTTACTTTTTCCAAGTTAATATATATAGACAATGAATGGTTGTGTTGTGTGTGACAAARTTTCTGTGACCTGTGACCTCACATTAAGGTTGTGTTGTATGGGATCTGTCAATTTCAGACAGTAGTATTATGATTGTAGTCGCAACCGCATCGGTCTCTACATTAGGAATTTGTGTTGTGGATGTAAATCACCTAAAAATGTGAATTATTGCTCACATTAATTCTTATTTTATCTTTCAATTTAATAAAAAGTT
TARGET=1047,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02271 Ps_contig_mira-and-tgicl-ass_franssen_19698
SEQUENCE=AAAGAACAAGTAGGTAATTCAAACGCAGAGTCCGCGGGGATCCTTCACTACCAAATATCACATTTCAATTCTTCTTGAGTTCAGTACAATGGATTCCAGAAAAGCAATGCTCATTCTTGGCCTATTGGCCATGGTTCTTCTTATTTCATCAGAGGTGTCAGCTAGGGAATTAACTGAGGAGGTTGTTGAAAAGTCAGATGAAGTAAACGATGCTAAATATGGAGGTTATGGACGTGGTGGTGGTTACCATAATGGAGGCGGTTACCACAATGGTGGAGGAGGATACAATGGCGGTGGCGGTTACCACAATGGTGGAGGAGGATACAATGGCGGTGGCGGTTACCACAATGGTGGAGGTGGTTACCATAATGGTGGAGGTGGTTACAATGGCGGTGGAGGTTACCATAATGGTGGTGGTGGTTACCATGGTGGAGGTGGACACGGTGGACATGGTGGTGCTTCCAACAACGGTAACTGATAATATCAACATTCTCAGTAATAAATATCATGAATTAATAATGTTGGATGTTGAAAAAAAAAGAGAGGTATAGATAGACATGAATCTTTTATGGTTATGTATTATCTATCTTGCAACTCTTTAGTATAGTTATTTCTCAACTCTATAAATCACTATGTATATGTGATGGATGATGAGTTTTATTTTGTACCAAAGTGTTGAATGTTCTATCATGTGTAATATATCAAATTTCATCTTTAGAGTGCATCTTCTTTAGGTAGTAAAAAAATTTGCCAMWAWYTTAACC
TARGET=409,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02272 Ps_contig_mira-and-tgicl-ass_franssen_19701
SEQUENCE=CGTTCAACGCAGAGTCGGGGAAACAAGAAAGCAGATAACAGAAAAAAAGGTGTGAGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGCAGCAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTGCTCTGCTTTGCCTTATCTCCCTCCTCCTCGCCTCTCCACCACCACTCCTTCTTCTTCCCCTTTCCCTAAACATTTTTCAGAGTCGCGGAAGTTTTCTCTTCATCGGACTAGAGCTTCTTCTTCTGATGATACATCTACTTCTCTCGATACCAATGAGTTGTTTACCGATTTGAAGGAGAAGTGGGATGGTCTTGAAAACAAGTCCACAGTGCTTCTGTATGGTGGTGGTGGTTTGGTTGCTGTTTGGTTATCTTCAATTCTTGTAGGTGCCATCAACTCAGTCCCCTTGCTTCCAAAGATTATGGAGTTGGTAGGACTAGGGTACACTGGATGGTTTGTCTACCGGTACCTTCTGTTTAAGTCTAGTAGAAAGGAACTAGCTACGGATATTGAGGCACTGAAAAAGAAAATTGCCGGAACCGAATAGAGCTGTATTAGTAGCAAGTCATGTTGATCTTCCTTGCTCATCATTTGTTTGTTTTGCTGTATGTCTTGTTATATGAGAGGCATTTGTATCTCTTGATATAGAATAGAATTGCTCAATCTCTATCAGTATCAACCTTGTATTAATCCATTTCATTGTTGAAAATAATGCAGCAAATCTTATATATATGTTTATGATTCTGAATGAAAATCATTCATTAAAAATTTTTAGG
TARGET=54,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4419
PRIMER_LEFT_PENALTY=0.390906
PRIMER_RIGHT_PENALTY=0.051029
PRIMER_LEFT_SEQUENCE=GGGGAAACAAGAAAGCAGATAA
PRIMER_RIGHT_SEQUENCE=GAAAAATGTTTAGGGAAAGGGG
PRIMER_LEFT=16,22
PRIMER_RIGHT=229,22
PRIMER_LEFT_TM=59.609
PRIMER_RIGHT_TM=60.051
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=214
PRIMER_PAIR_PENALTY_1=0.4454
PRIMER_LEFT_1_PENALTY=0.390906
PRIMER_RIGHT_1_PENALTY=0.054491
PRIMER_LEFT_1_SEQUENCE=GGGGAAACAAGAAAGCAGATAA
PRIMER_RIGHT_1_SEQUENCE=GTTTAGGGAAAGGGGAAGAAGA
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=222,22
PRIMER_LEFT_1_TM=59.609
PRIMER_RIGHT_1_TM=59.946
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.4538
PRIMER_LEFT_2_PENALTY=0.390906
PRIMER_RIGHT_2_PENALTY=0.062929
PRIMER_LEFT_2_SEQUENCE=GGGGAAACAAGAAAGCAGATAA
PRIMER_RIGHT_2_SEQUENCE=TTAGGGAAAGGGGAAGAAGAAG
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=220,22
PRIMER_LEFT_2_TM=59.609
PRIMER_RIGHT_2_TM=60.063
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.5639
PRIMER_LEFT_3_PENALTY=0.390906
PRIMER_RIGHT_3_PENALTY=0.173020
PRIMER_LEFT_3_SEQUENCE=GGGGAAACAAGAAAGCAGATAA
PRIMER_RIGHT_3_SEQUENCE=ATGTTTAGGGAAAGGGGAAGAA
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=224,22
PRIMER_LEFT_3_TM=59.609
PRIMER_RIGHT_3_TM=60.173
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.5639
PRIMER_LEFT_4_PENALTY=0.390906
PRIMER_RIGHT_4_PENALTY=0.173020
PRIMER_LEFT_4_SEQUENCE=GGGGAAACAAGAAAGCAGATAA
PRIMER_RIGHT_4_SEQUENCE=AATGTTTAGGGAAAGGGGAAGA
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=225,22
PRIMER_LEFT_4_TM=59.609
PRIMER_RIGHT_4_TM=60.173
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_02273 Ps_contig_mira-and-tgicl-ass_franssen_19701
SEQUENCE=CGTTCAACGCAGAGTCGGGGAAACAAGAAAGCAGATAACAGAAAAAAAGGTGTGAGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGCAGCAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTGCTCTGCTTTGCCTTATCTCCCTCCTCCTCGCCTCTCCACCACCACTCCTTCTTCTTCCCCTTTCCCTAAACATTTTTCAGAGTCGCGGAAGTTTTCTCTTCATCGGACTAGAGCTTCTTCTTCTGATGATACATCTACTTCTCTCGATACCAATGAGTTGTTTACCGATTTGAAGGAGAAGTGGGATGGTCTTGAAAACAAGTCCACAGTGCTTCTGTATGGTGGTGGTGGTTTGGTTGCTGTTTGGTTATCTTCAATTCTTGTAGGTGCCATCAACTCAGTCCCCTTGCTTCCAAAGATTATGGAGTTGGTAGGACTAGGGTACACTGGATGGTTTGTCTACCGGTACCTTCTGTTTAAGTCTAGTAGAAAGGAACTAGCTACGGATATTGAGGCACTGAAAAAGAAAATTGCCGGAACCGAATAGAGCTGTATTAGTAGCAAGTCATGTTGATCTTCCTTGCTCATCATTTGTTTGTTTTGCTGTATGTCTTGTTATATGAGAGGCATTTGTATCTCTTGATATAGAATAGAATTGCTCAATCTCTATCAGTATCAACCTTGTATTAATCCATTTCATTGTTGAAAATAATGCAGCAAATCTTATATATATGTTTATGATTCTGAATGAAAATCATTCATTAAAAATTTTTAGG
TARGET=372,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0271
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=AGAAGTGGGATGGTCTTGAAAA
PRIMER_RIGHT_SEQUENCE=TTGATGGCACCTACAAGAATTG
PRIMER_LEFT=328,22
PRIMER_RIGHT=427,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=0.0581
PRIMER_LEFT_1_PENALTY=0.054491
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=TCTTCTTCCCCTTTCCCTAAAC
PRIMER_RIGHT_1_SEQUENCE=TTGATGGCACCTACAAGAATTG
PRIMER_LEFT_1=201,22
PRIMER_RIGHT_1=427,22
PRIMER_LEFT_1_TM=59.946
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=227
PRIMER_PAIR_PENALTY_2=0.0635
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.003589
PRIMER_LEFT_2_SEQUENCE=GATTTGAAGGAGAAGTGGGATG
PRIMER_RIGHT_2_SEQUENCE=TTGATGGCACCTACAAGAATTG
PRIMER_LEFT_2=318,22
PRIMER_RIGHT_2=427,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=110
PRIMER_PAIR_PENALTY_3=0.0665
PRIMER_LEFT_3_PENALTY=0.062929
PRIMER_RIGHT_3_PENALTY=0.003589
PRIMER_LEFT_3_SEQUENCE=CTTCTTCTTCCCCTTTCCCTAA
PRIMER_RIGHT_3_SEQUENCE=TTGATGGCACCTACAAGAATTG
PRIMER_LEFT_3=199,22
PRIMER_RIGHT_3=427,22
PRIMER_LEFT_3_TM=60.063
PRIMER_RIGHT_3_TM=59.996
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=0.1766
PRIMER_LEFT_4_PENALTY=0.173020
PRIMER_RIGHT_4_PENALTY=0.003589
PRIMER_LEFT_4_SEQUENCE=TTCTTCCCCTTTCCCTAAACAT
PRIMER_RIGHT_4_SEQUENCE=TTGATGGCACCTACAAGAATTG
PRIMER_LEFT_4=203,22
PRIMER_RIGHT_4=427,22
PRIMER_LEFT_4_TM=60.173
PRIMER_RIGHT_4_TM=59.996
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_02274 Ps_contig_mira-and-tgicl-ass_franssen_19706
SEQUENCE=TTTTACTAAGTTAGGTTTGGTTGGATAGAAGGTTTAACCGTTACGTAAACCTACCAATTTGTTAAACTAACCAATTAATTAMMTTTTCTAATTACAGAACAATTAAGACTATATATTAAAAGTTTTAGATCAAACATCAAACACTGCAACTGTTTTATTTTCAAAATACATAGAAACTTTTAGCATAGTACTAGAAAGCTAGACATGACAATGTTGTTAAGCAGAAAGACCAGCTTCTTCGAGCAGCTGTTTCACCTTAGTGATGTAATCACTCATTGCTTCATCCTTGGATTTTCCTTCAACAGCCTTCCATGCATCCCATTTAGCTCTGTCTTTCTGGCTGAAAATCCCAGGACGAGCGGTGGTAACAGGTCCAAGAGTGGCTTGCTTGTACAATCCATAAAGGATAAGCAAGTTTTCATTTGATGGACTCTCTGTTAGAGTCTTAACTTTCGCAGCATGCTCCTCAAAATCCTCCTGCAAACCCATGATGTGTGTGTGTTTTGGTTGGGAGTGAAAGTGAGAGAGATATGAAGAAAGATCCCCGACTTTCGAAGCTCGTCCCCGAC
TARGET=492,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02275 Ps_contig_mira-and-tgicl-ass_franssen_19707
SEQUENCE=GAAACACTACCCATCACCATATATTTCCTAATCTTATCATACYMKRKGATACATCTAACCTCTGAAAAACCCACTCAAACTTACCAATAATCTAATCAACACCAAACACAAACAAACAAACGCAAAAAACGTCAACCACACGAAACCTCCTCATAATTCCTTCCCCTCCCTAAAACCATTCTATATTTCCTTCTCTCACCTCACCTCACAACAACACTTCCACTCTCACCTTTCTCCAAAACATTATCATAATTCTTCTTCTTTATTCATATTTTTCATCTATCACACTACTACACTTCACACTCTCAACATCATGTCGGTTAAGGAGTGCGATCACCACAAAGGAAGAAAAAGAATACTCTTCCAAAGAATATTTTGGGGAATCATAGTGTTCCTTTTCTTAGTTTTACTTACAATTCTCCTCATATGGGCAATCCTAAGACCCACAAAACCAACCTTCATCCTCTCCGACATAACCGTCTCCGCCTTCAACACAACGCAACCGAATCTCCTAACGACCAATTTCCAAATAACACTAATGTCAAGAAATCCCAACGACCACATCGGCGTCTACTACGACCGTCTCGACACCTACGTCACCTACCGTAGCCAGCAGATCACTTACAGAACCTCCATACCACCTTCCTACCAAGGTCACAAAGAAGTCGACGTTTGGTCGCCGGCGGTTTACGGCACCGAAGTTCCCGTTGCTCCGTTTAACTTTCAGACTCTCTCGCAGGATCAATCTAACGGTAATTTCTTTGCTACCGTTAGAGTTGACGGAAGAGTTCGTTGGAAGGTTGGTACGTTTATTTCTGGTCATTATCATCTCTATGTTCGGTGTCCTGCTTATATCTCAGTTCGTAACGACGGTAACGGCGTTAACATGGGAGCTAACGGCGTTTATATTGGAGCAAACGTCGTTAAGTATCAGATGGATCAACGTTGCTCTGTTAGCCTTTGAAAGTAAACTATTACTGTCTGATTTAATTTCTCTCTTTATATTTTCTTGTATTGTATTTTATACGTATCTGTATAATAGTATATTCTATAGTTCTATATGTATAATTGTGTATTTGAACTTTTACAAAAAAAATATGAGAGATGAGAAGGTATAGAAGAGTTACATGTTGTACTTGTAATAACATATGCCAATAATATATGACGCAGTATATA
TARGET=108,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02276 Ps_contig_mira-and-tgicl-ass_franssen_19707
SEQUENCE=GAAACACTACCCATCACCATATATTTCCTAATCTTATCATACYMKRKGATACATCTAACCTCTGAAAAACCCACTCAAACTTACCAATAATCTAATCAACACCAAACACAAACAAACAAACGCAAAAAACGTCAACCACACGAAACCTCCTCATAATTCCTTCCCCTCCCTAAAACCATTCTATATTTCCTTCTCTCACCTCACCTCACAACAACACTTCCACTCTCACCTTTCTCCAAAACATTATCATAATTCTTCTTCTTTATTCATATTTTTCATCTATCACACTACTACACTTCACACTCTCAACATCATGTCGGTTAAGGAGTGCGATCACCACAAAGGAAGAAAAAGAATACTCTTCCAAAGAATATTTTGGGGAATCATAGTGTTCCTTTTCTTAGTTTTACTTACAATTCTCCTCATATGGGCAATCCTAAGACCCACAAAACCAACCTTCATCCTCTCCGACATAACCGTCTCCGCCTTCAACACAACGCAACCGAATCTCCTAACGACCAATTTCCAAATAACACTAATGTCAAGAAATCCCAACGACCACATCGGCGTCTACTACGACCGTCTCGACACCTACGTCACCTACCGTAGCCAGCAGATCACTTACAGAACCTCCATACCACCTTCCTACCAAGGTCACAAAGAAGTCGACGTTTGGTCGCCGGCGGTTTACGGCACCGAAGTTCCCGTTGCTCCGTTTAACTTTCAGACTCTCTCGCAGGATCAATCTAACGGTAATTTCTTTGCTACCGTTAGAGTTGACGGAAGAGTTCGTTGGAAGGTTGGTACGTTTATTTCTGGTCATTATCATCTCTATGTTCGGTGTCCTGCTTATATCTCAGTTCGTAACGACGGTAACGGCGTTAACATGGGAGCTAACGGCGTTTATATTGGAGCAAACGTCGTTAAGTATCAGATGGATCAACGTTGCTCTGTTAGCCTTTGAAAGTAAACTATTACTGTCTGATTTAATTTCTCTCTTTATATTTTCTTGTATTGTATTTTATACGTATCTGTATAATAGTATATTCTATAGTTCTATATGTATAATTGTGTATTTGAACTTTTACAAAAAAAATATGAGAGATGAGAAGGTATAGAAGAGTTACATGTTGTACTTGTAATAACATATGCCAATAATATATGACGCAGTATATA
TARGET=195,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02277 Ps_contig_mira-and-tgicl-ass_franssen_19709
SEQUENCE=TTTWTTAGTTTAGTAATAGAGTGAATGTTATATATTCTTGAACCAACATAGCATACATCATAGAATATTTTTTCTGAACCATACATGATTATCTTATAAAAGTACTTAAGAGTTTACAAGTAAAACGATATACATAATTTATAACAAACAAACACTATTATTTGTCCTCAATTTATTCACTCCCAAACAGCATAGTCTTTACCAGAAGCAACAACATGGAAATTTGATGGAACAAGATTTCCAAGATCCATCTTTGGCCCTATCTTCACAATAACCTTGTTGTCTATCTTTGCTACATATAAGTCACCATCAGCAGCTAGAATGTTCACACTGCTTTTCATATTTATCCCATTCTTCTGTCTTATTGCAGCCAATTTTGTTATCTCATCCTTCAAACCCCAATCAATGAAATGATCATAGAATATTGATGGTGTTCCGGGATGTGTTAGAATATACGCATAACCCTGAATGACTTTATCAGAAGGAAATGGCCAAAGTCTCTGAGTTGAACCTGTATCATGGTTATCAATAAACGTTACACCATTTTCGGGTTTTATACCGATCAATCCAGGTGGTTTTCCGTTCGAATCTTTCAACCTCCATAGTTCACCTTGTACAGCAGCTTGAAGAATCCCTTTGGTGGTAAAATCAAATGCGTTAACAACTCCGCCACCAGCATCGTCAACCCAGTTAGCAAGTTCACCTCTAGCCGCATCTTGATTATAGTTAATCTTCCCGTCATTACCGTTAGAAAGTGAGTTCCAATACTCTCCAACTGCAAAATCTGGTTTAGTTTGTTCCACGTAAATTTTGGTTATACTAGGAGCATAACCTTTGACGAAATCGAACCTCCAACCGGAAAATCCAATTTCGGTTTTGAGCCAGTTCATCCATTCAGATAACTCTCTTTGTACTTGTGGATTGAGATGATCAATGTCAGGTGCAGCTTGATAATCCTCTCCTGTATCGTCGTTTCCTTTGCCATCGGAATAAGTGGTGTCACCTTTGCAAATGAAAGATGGACCCCAATCAAGCTTTGAATCTGGAGTTCCACCTTCGAAGATGCAATAAATTCCTCTATCATCTTTTCTCTCGGCTGTTCTATGGTTGATGACTATGTCAGCTACAGACTTGATTCCTTTATCGTTGAGAGCTGCAATTAGTGACTTCAGTTCATCTTTTGTTCCATATTTTGATGCATCAAGATCATATAGCCTTCCAGGAAGATACCCTTGAGGTCCAACACTTTGAGAAGGAGGAGGGAGCCAAACATGTGTGATTCCGGCGTTTGCTAAGTCAGGAATGGTGTTCTTCAAAGAGTTGTACCATCCTCCTTTGTTACTTGATTCCCAGTTGAATCCCTGAAAGAGTAAAGTAGAAGAAGCTAAAAGTGGAAGAGATGAAAGGAAGAAGCAGAAAAGAGTGATGAAAGGATGAGAATCCATTGCTACAAATGTGTTGTGTTTTCCCCGCGTACTCTGCGTKGATACCA
TARGET=143,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02278 Ps_contig_mira-and-tgicl-ass_franssen_19710
SEQUENCE=AAATTCAAGAABTTTAGTTAATAAAACAAAATATGCTTCTGCTTCACAAACAAAATACATTCCACAATAATTTTTTGACACAATTTTTTCTTTTCAATCTAAAAAATATAATAAACCCAAACAAATTTTTCTTTTATGTGTATTTAAAAAAGTACCCACAAAGACAATTTAAGAACCAAATACATATACCATTAATTCAAGAAAGAAATCACCACACAAGGCCTTAATGATAGTAATTAATTAAACAAAACACCCCCAAAGCCACAAACCAACACAACCAATCGATCATATTCAATGAGAAGTAGTCTCATCCTTGTGATGATCTTTGTCTTCACCATCAGTAGCAGTCTTAGCATGATAACCAGGAAGCTTCTCTTTGATCTTTTCCAAAATACCTTTCTTCTCTCCCTCATGAATAGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTTGTTACTTCCTCTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTAATTTTGTCAAGGAAACCCTTCTTTTCCTCAGTACCGGTTGTTCCTTCAACAACCTCAACTTTCTCAACTGGCACCGAAGTGTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTTCTCACCATCTTCTCCTTCCTCTTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGGTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCACCTTGTGACCAAACTCGTGGGCAATCGCCTCATCTTGAGCAGGCTTGTGTTCTTCATCCTTTTTCTTACCACCGAGAAAATCAAAAACACCTCTGTCCTTGATCTCTGTCTCAGAGTTGGTTGCGGATGTAGTCTCCTCGTACTTGTTCTGATTCTCCTCAGCCATGATGATCACTACTAACAAGATGGATCGAACTGGAAATTAATTAACTATAACTAAAATGGATTATGTAGCTGTAACTCTGAAATGAAAGGTAAAGTATGAGACGATGATGAGAGTGTTGTTGTTGTTTGGATCTCCGAC
TARGET=622,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02279 Ps_contig_mira-and-tgicl-ass_franssen_19710
SEQUENCE=AAATTCAAGAABTTTAGTTAATAAAACAAAATATGCTTCTGCTTCACAAACAAAATACATTCCACAATAATTTTTTGACACAATTTTTTCTTTTCAATCTAAAAAATATAATAAACCCAAACAAATTTTTCTTTTATGTGTATTTAAAAAAGTACCCACAAAGACAATTTAAGAACCAAATACATATACCATTAATTCAAGAAAGAAATCACCACACAAGGCCTTAATGATAGTAATTAATTAAACAAAACACCCCCAAAGCCACAAACCAACACAACCAATCGATCATATTCAATGAGAAGTAGTCTCATCCTTGTGATGATCTTTGTCTTCACCATCAGTAGCAGTCTTAGCATGATAACCAGGAAGCTTCTCTTTGATCTTTTCCAAAATACCTTTCTTCTCTCCCTCATGAATAGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTTGTTACTTCCTCTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTAATTTTGTCAAGGAAACCCTTCTTTTCCTCAGTACCGGTTGTTCCTTCAACAACCTCAACTTTCTCAACTGGCACCGAAGTGTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTTCTCACCATCTTCTCCTTCCTCTTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGGTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCACCTTGTGACCAAACTCGTGGGCAATCGCCTCATCTTGAGCAGGCTTGTGTTCTTCATCCTTTTTCTTACCACCGAGAAAATCAAAAACACCTCTGTCCTTGATCTCTGTCTCAGAGTTGGTTGCGGATGTAGTCTCCTCGTACTTGTTCTGATTCTCCTCAGCCATGATGATCACTACTAACAAGATGGATCGAACTGGAAATTAATTAACTATAACTAAAATGGATTATGTAGCTGTAACTCTGAAATGAAAGGTAAAGTATGAGACGATGATGAGAGTGTTGTTGTTGTTTGGATCTCCGAC
TARGET=1056,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02280 Ps_contig_mira-and-tgicl-ass_franssen_19711
SEQUENCE=TCAACGCAGAGTACGTCGGGGACTACTCTTTCTTTCACATCTTTTCTCTCTTTCAAACAAGTCTTCCATTTCTAAACCCACAAAATGGCCTCCTCTGCAGCACTACAAGTTTTTCTTATCTTAGGGCTTCTAGCCACCTCTTGTTTTGCACAAGCCCCAACTGCTGCACCAACACAGCCTCCAACCGCAACCCCATCCCCAAAATCTCCAGCACCGGCAACACCGCCACCAGCAACACCACCACCTGCAACTCCACCAACTGCAACTCCTCCAGCAGCAACACCACCTGCAGCTGCTCCCACTCCAGCCACTCCAGCACCCGCCTCTTCTCCAGCTTCCTCCCCTCCAGCTCCAACACCAACCGGTGGACCTGCCTCTTCTGCCTCTCCTCCCGCACCTACTCCAACCTCCCCAGAAAGTGGTCCCGCCTCTTCACCAACCGATTCTCCACCTTCTCCCTCGGCAGCATTTTCCTTCAGCAAACCCATTGTTGCTGCAACTGCTCTCTCTGCTGCTTTCTTTGCCATCGTTTTCTAGGACTCGTCCACGTGGATCTTGATCTGACTTTTCATTGACGGACGGACGGGATCTGTTTGATTTAAATTCTGAGTAGCGTTTTATTTTGTTTTCATGTGTCTATTTATACTTTTTCGTTCATGTGTTTGTTGATTCGACGGACGGGATCGATCTTCATGATTATGATGATGATTTATTTTGTGATTCATTTTGCTTTGGTGTTCACACGTGTATGTAGATGACCGTGATGATTTCATTGTACATCCATTAATTCATAGAATAAAATTGTTTGTCATTAAACTTTTTWAAAAAAAA
TARGET=574,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02281 Ps_contig_mira-and-tgicl-ass_franssen_19756
SEQUENCE=GGAAAGCAGATAACAGAAAAAAAGGTGTGAGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGCAGCAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACTCGCTGCTCTGCTTTGCCTTATCTCSSKYYTCYTCGCCTCTTCCACCACCACTCCTTCTTCTTCCCCYTTCCCTAAACATTTTTCAGAGTCGCGGAAGTTTTCTCTTCATCGGAACTAAG
TARGET=29,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02282 Ps_contig_mira-and-tgicl-ass_franssen_19780
SEQUENCE=AGGAGAAGCGTACTTGTATGGCTTCTTGGGTGGTGAGTAGACTGGGTGTGGGTGAGGATAGGTGTGAACTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGATATTTCAGATGGGAAACTGAAAAAGAGTATGGCTAATGCAAGAGGTCTTAATTATCATGGTTGTTGAGTGGATAGTAGTGCTTTTTGTTGCTTATTATATCGATCAAGTATTGTCGACAGGAAGTGGAAAAAATCCTCTATT
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9955
PRIMER_LEFT_PENALTY=0.028056
PRIMER_RIGHT_PENALTY=0.967416
PRIMER_LEFT_SEQUENCE=TGAGGATAGGTGTGAACTGGTG
PRIMER_RIGHT_SEQUENCE=TTAAGACCTCTTGCATTAGCCA
PRIMER_LEFT=52,22
PRIMER_RIGHT=218,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=59.033
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=1.0750
PRIMER_LEFT_1_PENALTY=0.028056
PRIMER_RIGHT_1_PENALTY=1.046971
PRIMER_LEFT_1_SEQUENCE=TGAGGATAGGTGTGAACTGGTG
PRIMER_RIGHT_1_SEQUENCE=TTCCACTTCCTGTCGACAATACT
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=296,23
PRIMER_LEFT_1_TM=60.028
PRIMER_RIGHT_1_TM=60.047
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=1.1419
PRIMER_LEFT_2_PENALTY=0.174518
PRIMER_RIGHT_2_PENALTY=0.967416
PRIMER_LEFT_2_SEQUENCE=GCTTCTTGGGTGGTGAGTAGAC
PRIMER_RIGHT_2_SEQUENCE=TTAAGACCTCTTGCATTAGCCA
PRIMER_LEFT_2=20,22
PRIMER_RIGHT_2=218,22
PRIMER_LEFT_2_TM=60.175
PRIMER_RIGHT_2_TM=59.033
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=1.1830
PRIMER_LEFT_3_PENALTY=0.215550
PRIMER_RIGHT_3_PENALTY=0.967416
PRIMER_LEFT_3_SEQUENCE=ATAGGTGTGAACTGGTGGAGGT
PRIMER_RIGHT_3_SEQUENCE=TTAAGACCTCTTGCATTAGCCA
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=218,22
PRIMER_LEFT_3_TM=59.784
PRIMER_RIGHT_3_TM=59.033
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=1.2215
PRIMER_LEFT_4_PENALTY=0.174518
PRIMER_RIGHT_4_PENALTY=1.046971
PRIMER_LEFT_4_SEQUENCE=GCTTCTTGGGTGGTGAGTAGAC
PRIMER_RIGHT_4_SEQUENCE=TTCCACTTCCTGTCGACAATACT
PRIMER_LEFT_4=20,22
PRIMER_RIGHT_4=296,23
PRIMER_LEFT_4_TM=60.175
PRIMER_RIGHT_4_TM=60.047
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=5.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=277
=
PRIMER_SEQUENCE_ID=SSR_02283 Ps_contig_mira-and-tgicl-ass_franssen_19786
SEQUENCE=GACTAGGTAACTTTTTCTTTTGCTATTTATTTATTAGAGAAATACATATAGTACAAACCTCATCATCAACCCAACCAAATTCCTACCCAAAACCACACTCAAACCGACACTAGTTCACACTTGACAACATMGYCTCACACATAGTAAAACTTAAAAACCNTATCACAACTACTTTATCATTCATTATTCGCATAAACAAAACCAACAGTAGAANATTATACAACTAATTAGAACCNGGTATTGACCAAACGTTA
TARGET=24,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02284 Ps_contig_mira-and-tgicl-ass_franssen_19794
SEQUENCE=TGGAGGAGAAGATTTATATTTGTAAATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACCGGTGGTGAATAAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTTGGATGGATATTTGTATGGAGGAGGNGGTGGAGATGGATTACTTATTATGGTGGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTTGACGTTTGTTTATTTTA
TARGET=194,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2910
PRIMER_LEFT_PENALTY=0.016290
PRIMER_RIGHT_PENALTY=0.274705
PRIMER_LEFT_SEQUENCE=TAAACCGGTGGTGAATAAAACC
PRIMER_RIGHT_SEQUENCE=CGGTATACAATTCACCTCCTCC
PRIMER_LEFT=67,22
PRIMER_RIGHT=310,22
PRIMER_LEFT_TM=59.984
PRIMER_RIGHT_TM=59.725
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.2965
PRIMER_LEFT_1_PENALTY=0.016290
PRIMER_RIGHT_1_PENALTY=0.280216
PRIMER_LEFT_1_SEQUENCE=TAAACCGGTGGTGAATAAAACC
PRIMER_RIGHT_1_SEQUENCE=CACCTCCTCCCACCATAATAAG
PRIMER_LEFT_1=67,22
PRIMER_RIGHT_1=298,22
PRIMER_LEFT_1_TM=59.984
PRIMER_RIGHT_1_TM=59.720
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.4097
PRIMER_LEFT_2_PENALTY=0.016290
PRIMER_RIGHT_2_PENALTY=0.393409
PRIMER_LEFT_2_SEQUENCE=TAAACCGGTGGTGAATAAAACC
PRIMER_RIGHT_2_SEQUENCE=TAAGTAATCCATCTCCACCNCC
PRIMER_LEFT_2=67,22
PRIMER_RIGHT_2=280,22
PRIMER_LEFT_2_TM=59.984
PRIMER_RIGHT_2_TM=59.607
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=11.0250
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.4884
PRIMER_LEFT_3_PENALTY=0.005574
PRIMER_RIGHT_3_PENALTY=0.482784
PRIMER_LEFT_3_SEQUENCE=GTGAATAGACAGGTGGAGGAGG
PRIMER_RIGHT_3_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=315,22
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=60.483
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.4884
PRIMER_LEFT_4_PENALTY=0.005574
PRIMER_RIGHT_4_PENALTY=0.482784
PRIMER_LEFT_4_SEQUENCE=GGTGAATAGACAGGTGGAGGAG
PRIMER_RIGHT_4_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=315,22
PRIMER_LEFT_4_TM=59.994
PRIMER_RIGHT_4_TM=60.483
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_02285 Ps_contig_mira-and-tgicl-ass_franssen_19794
SEQUENCE=TGGAGGAGAAGATTTATATTTGTAAATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACCGGTGGTGAATAAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTTGGATGGATATTTGTATGGAGGAGGNGGTGGAGATGGATTACTTATTATGGTGGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTTGACGTTTGTTTATTTTA
TARGET=225,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2910
PRIMER_LEFT_PENALTY=0.016290
PRIMER_RIGHT_PENALTY=0.274705
PRIMER_LEFT_SEQUENCE=TAAACCGGTGGTGAATAAAACC
PRIMER_RIGHT_SEQUENCE=CGGTATACAATTCACCTCCTCC
PRIMER_LEFT=67,22
PRIMER_RIGHT=310,22
PRIMER_LEFT_TM=59.984
PRIMER_RIGHT_TM=59.725
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.2965
PRIMER_LEFT_1_PENALTY=0.016290
PRIMER_RIGHT_1_PENALTY=0.280216
PRIMER_LEFT_1_SEQUENCE=TAAACCGGTGGTGAATAAAACC
PRIMER_RIGHT_1_SEQUENCE=CACCTCCTCCCACCATAATAAG
PRIMER_LEFT_1=67,22
PRIMER_RIGHT_1=298,22
PRIMER_LEFT_1_TM=59.984
PRIMER_RIGHT_1_TM=59.720
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.4097
PRIMER_LEFT_2_PENALTY=0.016290
PRIMER_RIGHT_2_PENALTY=0.393409
PRIMER_LEFT_2_SEQUENCE=TAAACCGGTGGTGAATAAAACC
PRIMER_RIGHT_2_SEQUENCE=TAAGTAATCCATCTCCACCNCC
PRIMER_LEFT_2=67,22
PRIMER_RIGHT_2=280,22
PRIMER_LEFT_2_TM=59.984
PRIMER_RIGHT_2_TM=59.607
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=11.0250
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.4884
PRIMER_LEFT_3_PENALTY=0.005574
PRIMER_RIGHT_3_PENALTY=0.482784
PRIMER_LEFT_3_SEQUENCE=GTGAATAGACAGGTGGAGGAGG
PRIMER_RIGHT_3_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=315,22
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=60.483
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.4884
PRIMER_LEFT_4_PENALTY=0.005574
PRIMER_RIGHT_4_PENALTY=0.482784
PRIMER_LEFT_4_SEQUENCE=GGTGAATAGACAGGTGGAGGAG
PRIMER_RIGHT_4_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=315,22
PRIMER_LEFT_4_TM=59.994
PRIMER_RIGHT_4_TM=60.483
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_02286 Ps_contig_mira-and-tgicl-ass_franssen_19797
SEQUENCE=ATGCATCGACGGTTGATTTTACTCCTCCGTTCCTTTCCCACTCCTTCTGATTGATTCGGTACTTTTTTGTATGTTGATGTGTTGTTGTTGTTCTCTTTTGTTGGATTTTGAATTGTATTGTTTAAGATATACTATTGTATTATTTCAGTTTTGTTTGTCAATTAACTACTGTGTCTTTTTAATAAWGTTTATATATGATGATGATAG
TARGET=80,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02287 Ps_contig_mira-and-tgicl-ass_franssen_19813
SEQUENCE=GTATCAACGCAGAGTACACGGGGAAGCAAACAAACATCAGAACAAAACCAAGCAATACACAACACAGTTTGCTGCAATGGCCTCTGCTCAGTGCTACAAATCCTGTGAACAAAGCTGCCAACACAAAAACAACTACCACCACCAGCAGCAACATAGCTCAATCGGACAGAAGGTAACGCTTCACAAAGGCCACCACAATGATGGAACACAATACCACCACCACCAGCAGCAGCAGCAGCAACATAACTCAATCGGACATAAAGGTAACTGACTT
TARGET=213,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4202
PRIMER_LEFT_PENALTY=0.057411
PRIMER_RIGHT_PENALTY=0.362762
PRIMER_LEFT_SEQUENCE=GCTCAGTGCTACAAATCCTGTG
PRIMER_RIGHT_SEQUENCE=TGTCCGATTGAGTTATGTTGCT
PRIMER_LEFT=85,22
PRIMER_RIGHT=257,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=59.637
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.4202
PRIMER_LEFT_1_PENALTY=0.057411
PRIMER_RIGHT_1_PENALTY=0.362762
PRIMER_LEFT_1_SEQUENCE=GCTCAGTGCTACAAATCCTGTG
PRIMER_RIGHT_1_SEQUENCE=GTCCGATTGAGTTATGTTGCTG
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=256,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=59.637
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.4202
PRIMER_LEFT_2_PENALTY=0.057411
PRIMER_RIGHT_2_PENALTY=0.362762
PRIMER_LEFT_2_SEQUENCE=TGCTCAGTGCTACAAATCCTGT
PRIMER_RIGHT_2_SEQUENCE=GTCCGATTGAGTTATGTTGCTG
PRIMER_LEFT_2=84,22
PRIMER_RIGHT_2=256,22
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=59.637
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.4202
PRIMER_LEFT_3_PENALTY=0.057411
PRIMER_RIGHT_3_PENALTY=0.362762
PRIMER_LEFT_3_SEQUENCE=TGCTCAGTGCTACAAATCCTGT
PRIMER_RIGHT_3_SEQUENCE=TGTCCGATTGAGTTATGTTGCT
PRIMER_LEFT_3=84,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=59.943
PRIMER_RIGHT_3_TM=59.637
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.4216
PRIMER_LEFT_4_PENALTY=0.058853
PRIMER_RIGHT_4_PENALTY=0.362762
PRIMER_LEFT_4_SEQUENCE=GCCAACACAAAAACAACTACCA
PRIMER_RIGHT_4_SEQUENCE=TGTCCGATTGAGTTATGTTGCT
PRIMER_LEFT_4=116,22
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=59.941
PRIMER_RIGHT_4_TM=59.637
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=142
=
PRIMER_SEQUENCE_ID=SSR_02288 Ps_contig_mira-and-tgicl-ass_franssen_19813
SEQUENCE=GTATCAACGCAGAGTACACGGGGAAGCAAACAAACATCAGAACAAAACCAAGCAATACACAACACAGTTTGCTGCAATGGCCTCTGCTCAGTGCTACAAATCCTGTGAACAAAGCTGCCAACACAAAAACAACTACCACCACCAGCAGCAACATAGCTCAATCGGACAGAAGGTAACGCTTCACAAAGGCCACCACAATGATGGAACACAATACCACCACCACCAGCAGCAGCAGCAGCAACATAACTCAATCGGACATAAAGGTAACTGACTT
TARGET=225,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9382
PRIMER_LEFT_PENALTY=0.057411
PRIMER_RIGHT_PENALTY=2.880772
PRIMER_LEFT_SEQUENCE=GCTCAGTGCTACAAATCCTGTG
PRIMER_RIGHT_SEQUENCE=CAGTTACCTTTATGTCCGATTGAG
PRIMER_LEFT=85,22
PRIMER_RIGHT=269,24
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=59.119
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=2.9382
PRIMER_LEFT_1_PENALTY=0.057411
PRIMER_RIGHT_1_PENALTY=2.880772
PRIMER_LEFT_1_SEQUENCE=TGCTCAGTGCTACAAATCCTGT
PRIMER_RIGHT_1_SEQUENCE=CAGTTACCTTTATGTCCGATTGAG
PRIMER_LEFT_1=84,22
PRIMER_RIGHT_1=269,24
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=59.119
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=2.9396
PRIMER_LEFT_2_PENALTY=0.058853
PRIMER_RIGHT_2_PENALTY=2.880772
PRIMER_LEFT_2_SEQUENCE=GCCAACACAAAAACAACTACCA
PRIMER_RIGHT_2_SEQUENCE=CAGTTACCTTTATGTCCGATTGAG
PRIMER_LEFT_2=116,22
PRIMER_RIGHT_2=269,24
PRIMER_LEFT_2_TM=59.941
PRIMER_RIGHT_2_TM=59.119
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=2.9396
PRIMER_LEFT_3_PENALTY=0.058853
PRIMER_RIGHT_3_PENALTY=2.880772
PRIMER_LEFT_3_SEQUENCE=TGCCAACACAAAAACAACTACC
PRIMER_RIGHT_3_SEQUENCE=CAGTTACCTTTATGTCCGATTGAG
PRIMER_LEFT_3=115,22
PRIMER_RIGHT_3=269,24
PRIMER_LEFT_3_TM=59.941
PRIMER_RIGHT_3_TM=59.119
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=2.9548
PRIMER_LEFT_4_PENALTY=0.073989
PRIMER_RIGHT_4_PENALTY=2.880772
PRIMER_LEFT_4_SEQUENCE=CTGCTCAGTGCTACAAATCCTG
PRIMER_RIGHT_4_SEQUENCE=CAGTTACCTTTATGTCCGATTGAG
PRIMER_LEFT_4=83,22
PRIMER_RIGHT_4=269,24
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=59.119
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_02289 Ps_contig_mira-and-tgicl-ass_franssen_19824
SEQUENCE=GTTTGTGGATGCTCCTTTTGTGTTTAAGCCAATTTGGCAGCTTACCAAGCCGTTGTTAAAATCATATGCTTCTCTGGTAAGATTTTGCTCTGCAGAGACTGTGAGGAAGGAATATTTTACAGATGAAACTTTGCCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCAGTACACTCACCTCCTCCACCTTATCATTACAATTCACCACCACCAGTTCACAAATACAAGTCACCACCTCCACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTA
TARGET=177,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1208
PRIMER_LEFT_PENALTY=0.115189
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=GCAGAGACTGTGAGGAAGGAAT
PRIMER_RIGHT_SEQUENCE=GATAAGGTGGAGGAGGTGAGTG
PRIMER_LEFT=91,22
PRIMER_RIGHT=214,22
PRIMER_LEFT_TM=59.885
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=0.1208
PRIMER_LEFT_1_PENALTY=0.115189
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=GCAGAGACTGTGAGGAAGGAAT
PRIMER_RIGHT_1_SEQUENCE=TGATAAGGTGGAGGAGGTGAGT
PRIMER_LEFT_1=91,22
PRIMER_RIGHT_1=215,22
PRIMER_LEFT_1_TM=59.885
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=0.1897
PRIMER_LEFT_2_PENALTY=0.115189
PRIMER_RIGHT_2_PENALTY=0.074466
PRIMER_LEFT_2_SEQUENCE=GCAGAGACTGTGAGGAAGGAAT
PRIMER_RIGHT_2_SEQUENCE=ATATGGTGGAGGAGGTGAATTG
PRIMER_LEFT_2=91,22
PRIMER_RIGHT_2=291,22
PRIMER_LEFT_2_TM=59.885
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.2239
PRIMER_LEFT_3_PENALTY=0.115189
PRIMER_RIGHT_3_PENALTY=0.108684
PRIMER_LEFT_3_SEQUENCE=GCAGAGACTGTGAGGAAGGAAT
PRIMER_RIGHT_3_SEQUENCE=TGGAGGTGGTGACTTGTATTTG
PRIMER_LEFT_3=91,22
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=59.885
PRIMER_RIGHT_3_TM=59.891
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.2617
PRIMER_LEFT_4_PENALTY=0.115189
PRIMER_RIGHT_4_PENALTY=0.146525
PRIMER_LEFT_4_SEQUENCE=GCAGAGACTGTGAGGAAGGAAT
PRIMER_RIGHT_4_SEQUENCE=CTGGTGGTGGTGAATTGTAATG
PRIMER_LEFT_4=91,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.885
PRIMER_RIGHT_4_TM=60.147
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_02290 Ps_contig_mira-and-tgicl-ass_franssen_19824
SEQUENCE=GTTTGTGGATGCTCCTTTTGTGTTTAAGCCAATTTGGCAGCTTACCAAGCCGTTGTTAAAATCATATGCTTCTCTGGTAAGATTTTGCTCTGCAGAGACTGTGAGGAAGGAATATTTTACAGATGAAACTTTGCCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCAGTACACTCACCTCCTCCACCTTATCATTACAATTCACCACCACCAGTTCACAAATACAAGTCACCACCTCCACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTA
TARGET=333,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.6564
PRIMER_LEFT_PENALTY=0.115189
PRIMER_RIGHT_PENALTY=2.541256
PRIMER_LEFT_SEQUENCE=GCAGAGACTGTGAGGAAGGAAT
PRIMER_RIGHT_SEQUENCE=AGGAGGAGGTGAGTTGTACTTG
PRIMER_LEFT=91,22
PRIMER_RIGHT=372,22
PRIMER_LEFT_TM=59.885
PRIMER_RIGHT_TM=57.459
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=2.7746
PRIMER_LEFT_1_PENALTY=0.115189
PRIMER_RIGHT_1_PENALTY=2.659428
PRIMER_LEFT_1_SEQUENCE=GCAGAGACTGTGAGGAAGGAAT
PRIMER_RIGHT_1_SEQUENCE=AGGAGGAGGTGAGTTGTACTTGT
PRIMER_LEFT_1=91,22
PRIMER_RIGHT_1=372,23
PRIMER_LEFT_1_TM=59.885
PRIMER_RIGHT_1_TM=58.341
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=2.8092
PRIMER_LEFT_2_PENALTY=0.115189
PRIMER_RIGHT_2_PENALTY=2.693970
PRIMER_LEFT_2_SEQUENCE=GCAGAGACTGTGAGGAAGGAAT
PRIMER_RIGHT_2_SEQUENCE=GGAGGAGGTGAGTTGTACTTGT
PRIMER_LEFT_2=91,22
PRIMER_RIGHT_2=371,22
PRIMER_LEFT_2_TM=59.885
PRIMER_RIGHT_2_TM=57.306
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=281
PRIMER_PAIR_PENALTY_3=3.0240
PRIMER_LEFT_3_PENALTY=0.482784
PRIMER_RIGHT_3_PENALTY=2.541256
PRIMER_LEFT_3_SEQUENCE=CACCTCCACCGGTATACAATTC
PRIMER_RIGHT_3_SEQUENCE=AGGAGGAGGTGAGTTGTACTTG
PRIMER_LEFT_3=254,22
PRIMER_RIGHT_3=372,22
PRIMER_LEFT_3_TM=60.483
PRIMER_RIGHT_3_TM=57.459
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=3.0755
PRIMER_LEFT_4_PENALTY=0.534252
PRIMER_RIGHT_4_PENALTY=2.541256
PRIMER_LEFT_4_SEQUENCE=TACAGATGAAACTTTGCCACCA
PRIMER_RIGHT_4_SEQUENCE=AGGAGGAGGTGAGTTGTACTTG
PRIMER_LEFT_4=117,22
PRIMER_RIGHT_4=372,22
PRIMER_LEFT_4_TM=60.534
PRIMER_RIGHT_4_TM=57.459
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_02291 Ps_contig_mira-and-tgicl-ass_franssen_19837
SEQUENCE=GTCGGGGACCATTGATAAGGATACAATCATCAAAAGCATAACAATCTTTTCAATTTCATTGCAATATAATACACGATGGCCGCATCATCATCATCATCCATGGCTCTCTCTTCTCCAACCTTGGCTGGCAAGCAACTCAAGCTGAACCCATCAAGCCAAGAATTGGGAGCTGCAAGGTTCACCATGGAAGGTAATTCCTCCTACCAACAAAG
TARGET=83,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5143
PRIMER_LEFT_PENALTY=0.090580
PRIMER_RIGHT_PENALTY=0.423703
PRIMER_LEFT_SEQUENCE=GGGGACCATTGATAAGGATACA
PRIMER_RIGHT_SEQUENCE=GGAATTACCTTCCATGGTGAAC
PRIMER_LEFT=3,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.909
PRIMER_RIGHT_TM=59.576
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.7513
PRIMER_LEFT_1_PENALTY=0.090580
PRIMER_RIGHT_1_PENALTY=0.660713
PRIMER_LEFT_1_SEQUENCE=GGGGACCATTGATAAGGATACA
PRIMER_RIGHT_1_SEQUENCE=CTTGATGGGTTCAGCTTGAGTT
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=154,22
PRIMER_LEFT_1_TM=59.909
PRIMER_RIGHT_1_TM=60.661
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.8442
PRIMER_LEFT_2_PENALTY=0.090580
PRIMER_RIGHT_2_PENALTY=0.753664
PRIMER_LEFT_2_SEQUENCE=GGGGACCATTGATAAGGATACA
PRIMER_RIGHT_2_SEQUENCE=AGGTTGGAGAAGAGAGAGCCAT
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=120,22
PRIMER_LEFT_2_TM=59.909
PRIMER_RIGHT_2_TM=60.754
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=1.0925
PRIMER_LEFT_3_PENALTY=0.090580
PRIMER_RIGHT_3_PENALTY=1.001941
PRIMER_LEFT_3_SEQUENCE=GGGGACCATTGATAAGGATACA
PRIMER_RIGHT_3_SEQUENCE=AGCCAAGGTTGGAGAAGAGAG
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=125,21
PRIMER_LEFT_3_TM=59.909
PRIMER_RIGHT_3_TM=60.002
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=123
PRIMER_PAIR_PENALTY_4=1.2588
PRIMER_LEFT_4_PENALTY=0.090580
PRIMER_RIGHT_4_PENALTY=1.168205
PRIMER_LEFT_4_SEQUENCE=GGGGACCATTGATAAGGATACA
PRIMER_RIGHT_4_SEQUENCE=GGTTGGAGAAGAGAGAGCCAT
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=119,21
PRIMER_LEFT_4_TM=59.909
PRIMER_RIGHT_4_TM=59.832
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=117
=
PRIMER_SEQUENCE_ID=SSR_02292 Ps_contig_mira-and-tgicl-ass_franssen_19846
SEQUENCE=AAGGTATTCATAAGAACTCATGATAGCATAATGAGTTCCAGCATTTTGTTGGAGCCTGGAAAACAGAATCAACCAACCCTTCCTCTTGTGATGTCTTGTTGGTCATCTGAAATATCATAGGCCAAACCCCTCCATCTGTCTCCGTCTGTCTGTCTTTTCAGGCTCGATCTCTGCAGCAGCAACTAATTTTGAAACTTCCAGAGGAACCTTAGTGTTTGGAAGCCTTGAAATTAATTCTTCTTCAAGTCTTTGTTAACCG
TARGET=144,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0686
PRIMER_LEFT_PENALTY=0.042192
PRIMER_RIGHT_PENALTY=0.026443
PRIMER_LEFT_SEQUENCE=TGAAATATCATAGGCCAAACCC
PRIMER_RIGHT_SEQUENCE=CCAAACACTAAGGTTCCTCTGG
PRIMER_LEFT=107,22
PRIMER_RIGHT=218,22
PRIMER_LEFT_TM=60.042
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=112
PRIMER_PAIR_PENALTY_1=0.0841
PRIMER_LEFT_1_PENALTY=0.057688
PRIMER_RIGHT_1_PENALTY=0.026443
PRIMER_LEFT_1_SEQUENCE=AAATATCATAGGCCAAACCCCT
PRIMER_RIGHT_1_SEQUENCE=CCAAACACTAAGGTTCCTCTGG
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=218,22
PRIMER_LEFT_1_TM=59.942
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=110
PRIMER_PAIR_PENALTY_2=0.1430
PRIMER_LEFT_2_PENALTY=0.116582
PRIMER_RIGHT_2_PENALTY=0.026443
PRIMER_LEFT_2_SEQUENCE=GAGCCTGGAAAACAGAATCAAC
PRIMER_RIGHT_2_SEQUENCE=CCAAACACTAAGGTTCCTCTGG
PRIMER_LEFT_2=51,22
PRIMER_RIGHT_2=218,22
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.1750
PRIMER_LEFT_3_PENALTY=0.148509
PRIMER_RIGHT_3_PENALTY=0.026443
PRIMER_LEFT_3_SEQUENCE=TCCTCTTGTGATGTCTTGTTGG
PRIMER_RIGHT_3_SEQUENCE=CCAAACACTAAGGTTCCTCTGG
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=218,22
PRIMER_LEFT_3_TM=60.149
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=0.3520
PRIMER_LEFT_4_PENALTY=0.042192
PRIMER_RIGHT_4_PENALTY=0.309780
PRIMER_LEFT_4_SEQUENCE=TGAAATATCATAGGCCAAACCC
PRIMER_RIGHT_4_SEQUENCE=CAAGGCTTCCAAACACTAAGGT
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=226,22
PRIMER_LEFT_4_TM=60.042
PRIMER_RIGHT_4_TM=59.690
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_02293 Ps_contig_mira-and-tgicl-ass_franssen_19849
SEQUENCE=TCGGGGATGTACRATAAGTCTCATTCACAACAAACGCATATCACAAACACCTTCTCTCTCTCCCTCTCTCCCAAAMCCTAGTTACAGATGCTGATAACCTTACTCCAAAGGTTATTGATGCCATCAAAAGTATCGAAATTGTTGAAGGAAACGGTGGTGCTGGAACCATCAAAAAACTCACTTTCG
TARGET=53,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02294 Ps_contig_mira-and-tgicl-ass_franssen_19856
SEQUENCE=GACGAGGAGGTGGTGGAGATCTTATCCTAGGTGGTGGTGGTGGAGGAGAACTTCTCCTAGGTGGTGGAGATCTTCTCTTACTTTCCTCTAGAGGTGTAGATGAAGGATATGAATTGCTGAGACACGCAACAGGGGATAGGCAAGGC
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5888
PRIMER_LEFT_PENALTY=1.330540
PRIMER_RIGHT_PENALTY=0.258273
PRIMER_LEFT_SEQUENCE=GAGGAGGTGGTGGAGATCTTATC
PRIMER_RIGHT_SEQUENCE=GCGTGTCTCAGCAATTCATATC
PRIMER_LEFT=3,23
PRIMER_RIGHT=126,22
PRIMER_LEFT_TM=60.331
PRIMER_RIGHT_TM=59.742
PRIMER_LEFT_GC_PERCENT=52.174
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=5.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=1.7074
PRIMER_LEFT_1_PENALTY=1.449085
PRIMER_RIGHT_1_PENALTY=0.258273
PRIMER_LEFT_1_SEQUENCE=GAGGTGGTGGAGATCTTATCCT
PRIMER_RIGHT_1_SEQUENCE=GCGTGTCTCAGCAATTCATATC
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=126,22
PRIMER_LEFT_1_TM=58.551
PRIMER_RIGHT_1_TM=59.742
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=5.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=121
PRIMER_PAIR_PENALTY_2=1.7074
PRIMER_LEFT_2_PENALTY=1.449085
PRIMER_RIGHT_2_PENALTY=0.258273
PRIMER_LEFT_2_SEQUENCE=AGGAGGTGGTGGAGATCTTATC
PRIMER_RIGHT_2_SEQUENCE=GCGTGTCTCAGCAATTCATATC
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=126,22
PRIMER_LEFT_2_TM=58.551
PRIMER_RIGHT_2_TM=59.742
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=5.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=1.7074
PRIMER_LEFT_3_PENALTY=1.449085
PRIMER_RIGHT_3_PENALTY=0.258273
PRIMER_LEFT_3_SEQUENCE=GAGGAGGTGGTGGAGATCTTAT
PRIMER_RIGHT_3_SEQUENCE=GCGTGTCTCAGCAATTCATATC
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=126,22
PRIMER_LEFT_3_TM=58.551
PRIMER_RIGHT_3_TM=59.742
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=5.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=1.7334
PRIMER_LEFT_4_PENALTY=1.330540
PRIMER_RIGHT_4_PENALTY=0.402858
PRIMER_LEFT_4_SEQUENCE=GAGGAGGTGGTGGAGATCTTATC
PRIMER_RIGHT_4_SEQUENCE=CGTGTCTCAGCAATTCATATCC
PRIMER_LEFT_4=3,23
PRIMER_RIGHT_4=125,22
PRIMER_LEFT_4_TM=60.331
PRIMER_RIGHT_4_TM=59.597
PRIMER_LEFT_4_GC_PERCENT=52.174
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_02295 Ps_contig_mira-and-tgicl-ass_franssen_19880
SEQUENCE=AAGCAGTGGTTATTCTAACGCAGAGTACGCGGGATCTTTCTTCATATCTCTCTACAACTTTCACTCCCAACCAAAACACACACACATCATGGGTTTKCAGGAGGTATTTTGAGGAGCATGCTGCGAAAGTTAAGACTCTAACAGAGAGTCCATCAAATGAAAACTTGCTTATCCTTTATGGATTGTACAAGCAAGCCACTCTTGGACCTTCTCCCTCTGATTCCCCTTCCCTTAAGTCCGAATCAAAGAACAACTACAAAACTTAAAA
TARGET=76,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02296 Ps_contig_mira-and-tgicl-ass_franssen_19912
SEQUENCE=GGGAACAGAAAAAAAAGGTGTGAGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGGCAGACAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTAGCTCTGTCTTTGTCCTTATCTCCCTCCTCCTCGCCTCCTCCACCACCACCTTCCTTTCCTTTCCTTTCCCCTTTACCCTAAACTTATTTTTCAGTAGTCGGACGGTAACGTTTTCTCTTCA
TARGET=22,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02297 Ps_contig_mira-and-tgicl-ass_franssen_19912
SEQUENCE=GGGAACAGAAAAAAAAGGTGTGAGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGGCAGACAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTAGCTCTGTCTTTGTCCTTATCTCCCTCCTCCTCGCCTCCTCCACCACCACCTTCCTTTCCTTTCCTTTCCCCTTTACCCTAAACTTATTTTTCAGTAGTCGGACGGTAACGTTTTCTCTTCA
TARGET=173,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2484
PRIMER_LEFT_PENALTY=0.193133
PRIMER_RIGHT_PENALTY=0.055230
PRIMER_LEFT_SEQUENCE=GCACAGTGTTAGTCCCTCCTCT
PRIMER_RIGHT_SEQUENCE=AGAAAACGTTACCGTCCGACTA
PRIMER_LEFT=70,22
PRIMER_RIGHT=237,22
PRIMER_LEFT_TM=59.807
PRIMER_RIGHT_TM=60.055
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.2484
PRIMER_LEFT_1_PENALTY=0.193133
PRIMER_RIGHT_1_PENALTY=0.055230
PRIMER_LEFT_1_SEQUENCE=AGCACAGTGTTAGTCCCTCCTC
PRIMER_RIGHT_1_SEQUENCE=AGAAAACGTTACCGTCCGACTA
PRIMER_LEFT_1=69,22
PRIMER_RIGHT_1=237,22
PRIMER_LEFT_1_TM=59.807
PRIMER_RIGHT_1_TM=60.055
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.2595
PRIMER_LEFT_2_PENALTY=0.193133
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=GCACAGTGTTAGTCCCTCCTCT
PRIMER_RIGHT_2_SEQUENCE=GAAAACGTTACCGTCCGACTAC
PRIMER_LEFT_2=70,22
PRIMER_RIGHT_2=236,22
PRIMER_LEFT_2_TM=59.807
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.2595
PRIMER_LEFT_3_PENALTY=0.193133
PRIMER_RIGHT_3_PENALTY=0.066394
PRIMER_LEFT_3_SEQUENCE=AGCACAGTGTTAGTCCCTCCTC
PRIMER_RIGHT_3_SEQUENCE=GAAAACGTTACCGTCCGACTAC
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=236,22
PRIMER_LEFT_3_TM=59.807
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=0.2607
PRIMER_LEFT_4_PENALTY=0.205455
PRIMER_RIGHT_4_PENALTY=0.055230
PRIMER_LEFT_4_SEQUENCE=CGCTAGCTCTGTCTTTGTCCTT
PRIMER_RIGHT_4_SEQUENCE=AGAAAACGTTACCGTCCGACTA
PRIMER_LEFT_4=117,22
PRIMER_RIGHT_4=237,22
PRIMER_LEFT_4_TM=60.205
PRIMER_RIGHT_4_TM=60.055
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_02298 Ps_contig_mira-and-tgicl-ass_franssen_19919
SEQUENCE=GGTACTATCCTTTTCTTAGAAAATTATCTTATAGTATTTTAACTTGTCTTTGTGAAACATGGGTGGCAAAGGTGGTAGCGGTGGTGGRGCTAAAGGAGGCGGTGGTGGAGATGCAAAAGGAGGCGGTGGTGGAAGCGGTGGTGGTGGTTACGGTCATGGCGGCGGTGGTGGATACAACGGTGGCGG
TARGET=137,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02299 Ps_contig_mira-and-tgicl-ass_franssen_19939
SEQUENCE=CTGTTTCTTGTCAATTGGCCATACTTTCCCATAGAGGAAAACACACACACATCATGGGTTTGCAGGAGGATYTTGAGGAGCATGCTGACGAAAGTTAAGACTCTAACAGGAGACGTCCATACAATGAAAACTTGCTTATCCTTTATGGATTGTACAAGCAAGCCACTTCTTGGTACCGTTCGTTACCCGACCCGTCTCGTTCCCGTGGGTTAC
TARGET=41,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02300 Ps_contig_mira-and-tgicl-ass_franssen_19954
SEQUENCE=CCAAATACAAAATGCTTTAATCAAGGAACTTAACTTGTTGAATATCTCATACAAATAAACTATACATCATCATCATCATCATCATCATTCTTAAATAAACAATAAAAGGGGACAAAGAAAAGACAAATTAAACAACATAAACGAAAAACATAGTAATCTTTAATTAAACCAAAAGAAAACCGTTAGGGATCAATAACCATTGCTAGAGTGCAAGATATAATAAGCAANAATNTGACCTANACGAACCCCAAGCCAAAACNTCAAACGTAACGGTTAAACCCG
TARGET=65,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02301 Ps_contig_mira-and-tgicl-ass_franssen_19957
SEQUENCE=GGACCCTCACACACCGTGTCCTCGTCACTCAAACGTAAAACAACAAAACACAGTCTCTCTCTCTTTTCACCTATCGCGTCCCTCTTTCTCTCGGGCTCTCACTGCGTACAAAAAMMNCGAGAAGAGGAACAACATGCAGATTCTTCGACAAGTAACCTTTAAACCGGAAAAACCNTCACTCTCGGAGGTTGAAGAGCAGGCCGAACACCATCGAAC
TARGET=54,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02302 Ps_contig_mira-and-tgicl-ass_franssen_19974
SEQUENCE=CGGGGTTACTTTCCTTACTTTTCTTACTTTCTTTAGTTTTCCCTTTTTTCTTTCTTTCTTTAACTATTGGTAGTCCTCCTCAAAGKTCTATGCTTATCTCATCATTTGCATGTCTTTCATCTCCTCAGCTACMMCTATTCTTGGTTGTGGCTATTGTGGA
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02303 Ps_contig_mira-and-tgicl-ass_franssen_19993
SEQUENCE=CCTTGCCTATCACCTGTTGCGTGTCTCAGTTGTTGTTGTTGTTGTTGTCTACTGGTCCCTGTTGAAGTTTTTGTCCTCTTGCTTTCAATTCCACGYTCTGAATGGGCCTGTGTGTTTGGAATAATTGGAATGGATAAAAGAAGCCAAAAATGATAATGGGGTCCTCAAAACTGTAGATAAGCATGAATATGATTATGATTATGGTTATGGTTATGTATGTTATGTTAAGGGTGTATTGTATCCTCCATTTATTNTTTGTTATCTTTTGTTTTGTTNTTTATTCAGATTNTACT
TARGET=29,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02304 Ps_contig_mira-and-tgicl-ass_franssen_20011
SEQUENCE=GACACATCTTCTTCATAASATCTCATCTCACCAAAACAAAAACAAAAACCAAAAAAAAAGCAGATCCAGAACACAATCATCAAACAAACAAAAATGGGTGGTAAG
TARGET=81,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02305 Ps_contig_mira-and-tgicl-ass_franssen_20033
SEQUENCE=ACTACTAAAGCTAACAAAAGCAAGTGCATTAGAACCACCACTATTAATTTGGAAATGCAACAACCCTTGTGGAAAAACCATAACATCACCTTTGTAAAGTGTTTCAAGGTAAACARCATTGTCGGAAACAAAACCAGCACAGTATTCGTTTCCTTAGAAACCAAACCAAACCAATATCTCCGACGCCACCGAGGGTGNGTGT
TARGET=158,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02306 Ps_contig_mira-and-tgicl-ass_franssen_20042
SEQUENCE=CTAGAGAATTAGCTGAGACTTCCACTAATGCCAAAGAGGAAGTTGCTGAAAAGTCAAATGAAGTAAATGATGCCAAATATTACGGTGGAGGTTATGGTCATGGCTATGGTCATGGACATGGAGGCTATGGTCATGGACATGGAGGCTACGGTCATGGCGGCGGAGGTTACAACGGTGGTGGAGGAGGCTACAACCATGGTGGTGGTGGCTACAATGGTGGTGGAGGAGGATACAACCATGGAGGYGGTGGTTACAATGGTGGTGGAGGAGGCTACAACCATGGAGGTGGTGGTTATCGTGGTGGAGGTGGACATGGTGGTAGTTTCAACAATGGTAACTGATTATATCATCATGCATGCATGTGTTAAGTGTAATAAATAATGTTGGGTTTTCAACAAAAGAGTAAAGATAAATCACTATGTATATGTGAAGA
TARGET=197,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02307 Ps_contig_mira-and-tgicl-ass_franssen_20042
SEQUENCE=CTAGAGAATTAGCTGAGACTTCCACTAATGCCAAAGAGGAAGTTGCTGAAAAGTCAAATGAAGTAAATGATGCCAAATATTACGGTGGAGGTTATGGTCATGGCTATGGTCATGGACATGGAGGCTATGGTCATGGACATGGAGGCTACGGTCATGGCGGCGGAGGTTACAACGGTGGTGGAGGAGGCTACAACCATGGTGGTGGTGGCTACAATGGTGGTGGAGGAGGATACAACCATGGAGGYGGTGGTTACAATGGTGGTGGAGGAGGCTACAACCATGGAGGTGGTGGTTATCGTGGTGGAGGTGGACATGGTGGTAGTTTCAACAATGGTAACTGATTATATCATCATGCATGCATGTGTTAAGTGTAATAAATAATGTTGGGTTTTCAACAAAAGAGTAAAGATAAATCACTATGTATATGTGAAGA
TARGET=351,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02308 Ps_contig_mira-and-tgicl-ass_franssen_20141
SEQUENCE=GTAAAAATGACCACGCGGGGCAAGACAGTAAAATATGATCGCCATCGTATCAACTTGGACTTTTACAAGAAAAATGACCACGCGGAAAAGATTYGGCCTAATTAAGTATCTCAAATCTAACAAGAGGGGATTAGGGGGATAAATAATAMSMKAAYRAATAAAAAACAAAAACAGTTGCCATACAAACTAAACAAAAAAGNGAGAGACNCCATGGTTGGTTGGTGGNGGGTGGATGGACATGCTNTTNACTAACNCCCTA
TARGET=212,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02309 Ps_contig_mira-and-tgicl-ass_franssen_20173
SEQUENCE=CGTTAAAATGACACNCGGGCAGACNGTAAATATGATGCCATCGTGTCAACTTGGACTTTACAAGAAAATGACCACGCGGAAAAGATTCGGCCTAATTAAGTATCTCAAATCTAACAAGAGGGGATTAGGGGGGATAATAATAAGATAATAATAAAAAACAAAAACAAGTTGCCATCAAACTAACAAAAAAGAGAGAGACGCCATGGTTGGTTGGTTGGGTGGGGTGGGAATGGACGATGCTCTTCTATTACCAAAACTTGAAGAGGAGACCACCGGTGGGTTGCAAAAGAAAGGAGACAAACACCAGTGACTCAACTGCCATCAGCTTGAATAAGGATGTAGNGAGCAGATGTNTCT
TARGET=204,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2772
PRIMER_LEFT_PENALTY=0.080323
PRIMER_RIGHT_PENALTY=0.196906
PRIMER_LEFT_SEQUENCE=GCAGACNGTAAATATGATGCCA
PRIMER_RIGHT_SEQUENCE=TGAGTCACTGGTGTTTGTCTCC
PRIMER_LEFT=18,22
PRIMER_RIGHT=313,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.197
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=0.4990
PRIMER_LEFT_1_PENALTY=0.080323
PRIMER_RIGHT_1_PENALTY=0.418641
PRIMER_LEFT_1_SEQUENCE=GCAGACNGTAAATATGATGCCA
PRIMER_RIGHT_1_SEQUENCE=ATAGAAGAGCATCGTCCATTCC
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=247,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.581
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.7031
PRIMER_LEFT_2_PENALTY=0.080323
PRIMER_RIGHT_2_PENALTY=0.622772
PRIMER_LEFT_2_SEQUENCE=GCAGACNGTAAATATGATGCCA
PRIMER_RIGHT_2_SEQUENCE=TTGTCTCCTTTCTTTTGCAACC
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=299,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=60.623
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.7121
PRIMER_LEFT_3_PENALTY=0.080323
PRIMER_RIGHT_3_PENALTY=0.631747
PRIMER_LEFT_3_SEQUENCE=GCAGACNGTAAATATGATGCCA
PRIMER_RIGHT_3_SEQUENCE=TGGTCTCCTCTTCAAGTTTTGG
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=271,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=60.632
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.8389
PRIMER_LEFT_4_PENALTY=0.642030
PRIMER_RIGHT_4_PENALTY=0.196906
PRIMER_LEFT_4_SEQUENCE=GGCAGACNGTAAATATGATGCC
PRIMER_RIGHT_4_SEQUENCE=TGAGTCACTGGTGTTTGTCTCC
PRIMER_LEFT_4=17,22
PRIMER_RIGHT_4=313,22
PRIMER_LEFT_4_TM=60.642
PRIMER_RIGHT_4_TM=60.197
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.50
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_02310 Ps_contig_mira-and-tgicl-ass_franssen_20185
SEQUENCE=TCGGGCGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTWCGGKSRTGKCGGTGGTGGTGGATACAACGGTGGCGGTTGTTTYCMAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGACCAAATATGGCGGTGGACACTATGGTGGTGGACACTATGGAGGTGGACACTACGGTGGTGGTCACTACGGTCA
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02311 Ps_contig_mira-and-tgicl-ass_franssen_20185
SEQUENCE=TCGGGCGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTWCGGKSRTGKCGGTGGTGGTGGATACAACGGTGGCGGTTGTTTYCMAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGACCAAATATGGCGGTGGACACTATGGTGGTGGACACTATGGAGGTGGACACTACGGTGGTGGTCACTACGGTCA
TARGET=82,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02312 Ps_contig_mira-and-tgicl-ass_franssen_20186
SEQUENCE=CTAGTGGTGGTAAGGTGGAGGAGGTGGAGTTTGTTAGGTTAATAAGGCGTGGTGGGAGGGTGGGGGAGGTAAACCGGAAGGAGGAGGAGGAATGGTAAACTAGGGGTAGGGAACGAATGAGGAGGGTAGGTGTGAGCTGGTGRWSGYRGWGTGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGYGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGANATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGAATGGTAGACAGGGTGAGGATGGGGATAGGTGTGCACTGGTGGTGGTGGAAGCCTGGCTTGGCGTGGTTCG
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02313 Ps_contig_mira-and-tgicl-ass_franssen_20186
SEQUENCE=CTAGTGGTGGTAAGGTGGAGGAGGTGGAGTTTGTTAGGTTAATAAGGCGTGGTGGGAGGGTGGGGGAGGTAAACCGGAAGGAGGAGGAGGAATGGTAAACTAGGGGTAGGGAACGAATGAGGAGGGTAGGTGTGAGCTGGTGRWSGYRGWGTGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGYGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGANATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGAATGGTAGACAGGGTGAGGATGGGGATAGGTGTGCACTGGTGGTGGTGGAAGCCTGGCTTGGCGTGGTTCG
TARGET=187,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02314 Ps_contig_mira-and-tgicl-ass_franssen_20186
SEQUENCE=CTAGTGGTGGTAAGGTGGAGGAGGTGGAGTTTGTTAGGTTAATAAGGCGTGGTGGGAGGGTGGGGGAGGTAAACCGGAAGGAGGAGGAGGAATGGTAAACTAGGGGTAGGGAACGAATGAGGAGGGTAGGTGTGAGCTGGTGRWSGYRGWGTGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGYGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGANATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGAATGGTAGACAGGGTGAGGATGGGGATAGGTGTGCACTGGTGGTGGTGGAAGCCTGGCTTGGCGTGGTTCG
TARGET=241,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02315 Ps_contig_mira-and-tgicl-ass_franssen_20186
SEQUENCE=CTAGTGGTGGTAAGGTGGAGGAGGTGGAGTTTGTTAGGTTAATAAGGCGTGGTGGGAGGGTGGGGGAGGTAAACCGGAAGGAGGAGGAGGAATGGTAAACTAGGGGTAGGGAACGAATGAGGAGGGTAGGTGTGAGCTGGTGRWSGYRGWGTGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGYGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGANATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGAATGGTAGACAGGGTGAGGATGGGGATAGGTGTGCACTGGTGGTGGTGGAAGCCTGGCTTGGCGTGGTTCG
TARGET=292,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02316 Ps_contig_mira-and-tgicl-ass_franssen_20186
SEQUENCE=CTAGTGGTGGTAAGGTGGAGGAGGTGGAGTTTGTTAGGTTAATAAGGCGTGGTGGGAGGGTGGGGGAGGTAAACCGGAAGGAGGAGGAGGAATGGTAAACTAGGGGTAGGGAACGAATGAGGAGGGTAGGTGTGAGCTGGTGRWSGYRGWGTGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGYGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGANATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGAATGGTAGACAGGGTGAGGATGGGGATAGGTGTGCACTGGTGGTGGTGGAAGCCTGGCTTGGCGTGGTTCG
TARGET=332,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02317 Ps_contig_mira-and-tgicl-ass_franssen_20186
SEQUENCE=CTAGTGGTGGTAAGGTGGAGGAGGTGGAGTTTGTTAGGTTAATAAGGCGTGGTGGGAGGGTGGGGGAGGTAAACCGGAAGGAGGAGGAGGAATGGTAAACTAGGGGTAGGGAACGAATGAGGAGGGTAGGTGTGAGCTGGTGRWSGYRGWGTGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGYGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGANATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGAATGGTAGACAGGGTGAGGATGGGGATAGGTGTGCACTGGTGGTGGTGGAAGCCTGGCTTGGCGTGGTTCG
TARGET=383,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02318 Ps_contig_mira-and-tgicl-ass_franssen_20189
SEQUENCE=TCTCACCGTGATTCAAACCCTTCGCCTTCTTCCTATCTCCACAATCGATTCCACCGCCACCGTTGTAACCACCGCCTMMACTRSTAVBATMVTCCACCDCCACCATHGYAYMACCACCACCTCCATGGTTGTATCCTCCTCCACCACCATTGTAGCCACCACCACCATGGTTGTAGCCTCCTCCACCACCGTTGTAACCTCCGCCGCCACCATGGTTGTAACCTCCACCGTAATATTTGGCATCATTTAATTCATCTGACTTTTCAACAACGTCATCTTTGGTATTAGTTGAAGTCTCGGTTAAGTCCCTAGCTGACACCTCTGAGGAAATTAGAAGAACAATGGCCATTAGGCCAAGGATGAGAATTGCTATTTTGGAATCCATTGTACTCAACTCAAGCTCAATTGAAATGTGATATTTAGTAGYGAAGGRTCCACGCGTACTCTGCGTTGATA
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02319 Ps_contig_mira-and-tgicl-ass_franssen_20201
SEQUENCE=AGGGTTGTCAGCGTAGGGAATTAACTGAGAGGTTGTTGAAAAGTCAGATGAAGTAAACGATGCTTAAATATGGAGGTTATGGACGTGGTGGTGGTTACCATAATGGAGGCGGTTACCACAATGGTGGAGGAGGATACAATGGCGGTGGCGGTTACCACAATGGTGGAGGAGGATACAATGGCGGTGGCGGTTACCACAATGGTGGAGGTGTACCATAATGGTGGAGGTGGTTACAATGGCGGTTGGAGGTTACCATAATGGTGGTGGTGGTTACCATGGTGGAGGTGGGCACGGTGGACATGGTGGTGCTTCCAACAACGGTAACTGATAATATCAACATTCTCAGTAATAAATATCATGAATTAATATGTGATGTAAGAAAAAAGNGAGGTANAGANAGACATAGTAATTTCTTTTAGTGGTTATGTTATTATACTTATTCTTAGACCTAACTTTA
TARGET=259,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4467
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.412596
PRIMER_LEFT_SEQUENCE=AATATGGAGGTTATGGACGTGG
PRIMER_RIGHT_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_LEFT=66,22
PRIMER_RIGHT=328,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.413
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.4871
PRIMER_LEFT_1_PENALTY=0.074466
PRIMER_RIGHT_1_PENALTY=0.412596
PRIMER_LEFT_1_SEQUENCE=CAATGGTGGAGGAGGATACAAT
PRIMER_RIGHT_1_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_LEFT_1=157,22
PRIMER_RIGHT_1=328,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.413
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.4871
PRIMER_LEFT_2_PENALTY=0.074466
PRIMER_RIGHT_2_PENALTY=0.412596
PRIMER_LEFT_2_SEQUENCE=CAATGGTGGAGGAGGATACAAT
PRIMER_RIGHT_2_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_LEFT_2=118,22
PRIMER_RIGHT_2=328,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=60.413
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.4871
PRIMER_LEFT_3_PENALTY=0.074466
PRIMER_RIGHT_3_PENALTY=0.412596
PRIMER_LEFT_3_SEQUENCE=AATGGTGGAGGAGGATACAATG
PRIMER_RIGHT_3_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_LEFT_3=158,22
PRIMER_RIGHT_3=328,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=60.413
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.4871
PRIMER_LEFT_4_PENALTY=0.074466
PRIMER_RIGHT_4_PENALTY=0.412596
PRIMER_LEFT_4_SEQUENCE=AATGGTGGAGGAGGATACAATG
PRIMER_RIGHT_4_SEQUENCE=ATCAGTTACCGTTGTTGGAAGC
PRIMER_LEFT_4=119,22
PRIMER_RIGHT_4=328,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=60.413
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_02320 Ps_contig_mira-and-tgicl-ass_franssen_20207
SEQUENCE=GGAGAAGAAAATGAAAGAAAATGGATCTTAAGCAAATTATCCCACTCAACCGTGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTYGTCTCAGTTACACCGTTGCATGTTCTAACATCCACTTGACCCTTTAAACCAAAGAAGAGTTTCTAGCTGTTCAAAACCATWACTCTTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCCCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACCTACCCTCATCCTCACCCAGTCTACCATTCCCCCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCAC
TARGET=238,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02321 Ps_contig_mira-and-tgicl-ass_franssen_20207
SEQUENCE=GGAGAAGAAAATGAAAGAAAATGGATCTTAAGCAAATTATCCCACTCAACCGTGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTYGTCTCAGTTACACCGTTGCATGTTCTAACATCCACTTGACCCTTTAAACCAAAGAAGAGTTTCTAGCTGTTCAAAACCATWACTCTTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCCCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACCTACCCTCATCCTCACCCAGTCTACCATTCCCCCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCAC
TARGET=403,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02322 Ps_contig_mira-and-tgicl-ass_franssen_20212
SEQUENCE=TTTTTTTTTTAGTTTTGATTGTCATTTCATACAATACATTAACTAGTTCAAATTTACAATATGAGCACATAAATAAGTTTTATAAGATCAACAATGAGTCAACAATAAAACACCCTCCTCTNTTGTNACACCCTTCTTCCAANCCATGATCAATACCATCCTTCTTTTTTCTTTGTTGGAAATAGAAGAAAAAACCTAAAAACCTATTCCCTTTTCGGGGCGGAACCGGTAAGGTTTCCTAACCCAACTTTTCAAAAACCTAAACCTTCCTTCCTTGGGTAAAGGACAACGACCAAACTAAACGGGTACCCCG
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5103
PRIMER_LEFT_PENALTY=0.074231
PRIMER_RIGHT_PENALTY=0.436048
PRIMER_LEFT_SEQUENCE=CAANCCATGATCAATACCATCC
PRIMER_RIGHT_SEQUENCE=CGTTTAGTTTGGTCGTTGTCCT
PRIMER_LEFT=139,22
PRIMER_RIGHT=303,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=60.436
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=0.5103
PRIMER_LEFT_1_PENALTY=0.074231
PRIMER_RIGHT_1_PENALTY=0.436048
PRIMER_LEFT_1_SEQUENCE=CCAANCCATGATCAATACCATC
PRIMER_RIGHT_1_SEQUENCE=CGTTTAGTTTGGTCGTTGTCCT
PRIMER_LEFT_1=138,22
PRIMER_RIGHT_1=303,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.436
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.75
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=166
PRIMER_PAIR_PENALTY_2=0.6412
PRIMER_LEFT_2_PENALTY=0.074231
PRIMER_RIGHT_2_PENALTY=0.566962
PRIMER_LEFT_2_SEQUENCE=CAANCCATGATCAATACCATCC
PRIMER_RIGHT_2_SEQUENCE=AGTTTGGTCGTTGTCCTTTACC
PRIMER_LEFT_2=139,22
PRIMER_RIGHT_2=298,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.433
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=0.6412
PRIMER_LEFT_3_PENALTY=0.074231
PRIMER_RIGHT_3_PENALTY=0.566962
PRIMER_LEFT_3_SEQUENCE=CCAANCCATGATCAATACCATC
PRIMER_RIGHT_3_SEQUENCE=AGTTTGGTCGTTGTCCTTTACC
PRIMER_LEFT_3=138,22
PRIMER_RIGHT_3=298,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.433
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.75
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=0.7271
PRIMER_LEFT_4_PENALTY=0.074231
PRIMER_RIGHT_4_PENALTY=0.652901
PRIMER_LEFT_4_SEQUENCE=CAANCCATGATCAATACCATCC
PRIMER_RIGHT_4_SEQUENCE=GTACCCGTTTAGTTTGGTCGTT
PRIMER_LEFT_4=139,22
PRIMER_RIGHT_4=308,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=59.347
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_02323 Ps_contig_mira-and-tgicl-ass_franssen_20257
SEQUENCE=AAAGACAAGTAGGTAATACAAACGACAAGAAGTACGTCGGGGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCGCCTCCACCAGCTCACACCTATCCTCACCCACA
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5790
PRIMER_LEFT_PENALTY=0.107763
PRIMER_RIGHT_PENALTY=0.471231
PRIMER_LEFT_SEQUENCE=ACAAGAAGTACGTCGGGGATAA
PRIMER_RIGHT_SEQUENCE=GCGGAGGAGATGAGTAATGGTA
PRIMER_LEFT=24,22
PRIMER_RIGHT=236,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=60.471
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.5790
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.471231
PRIMER_LEFT_1_SEQUENCE=CAAGAAGTACGTCGGGGATAAC
PRIMER_RIGHT_1_SEQUENCE=GCGGAGGAGATGAGTAATGGTA
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=236,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.471
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.6318
PRIMER_LEFT_2_PENALTY=0.160609
PRIMER_RIGHT_2_PENALTY=0.471231
PRIMER_LEFT_2_SEQUENCE=ATGGCTTCTGCTTCTCTCACTC
PRIMER_RIGHT_2_SEQUENCE=GCGGAGGAGATGAGTAATGGTA
PRIMER_LEFT_2=83,22
PRIMER_RIGHT_2=236,22
PRIMER_LEFT_2_TM=60.161
PRIMER_RIGHT_2_TM=60.471
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=0.6814
PRIMER_LEFT_3_PENALTY=0.107763
PRIMER_RIGHT_3_PENALTY=0.573649
PRIMER_LEFT_3_SEQUENCE=ACAAGAAGTACGTCGGGGATAA
PRIMER_RIGHT_3_SEQUENCE=GTGAGGATAGGTGTGAGCTGGT
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=60.574
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=0.6814
PRIMER_LEFT_4_PENALTY=0.107763
PRIMER_RIGHT_4_PENALTY=0.573649
PRIMER_LEFT_4_SEQUENCE=CAAGAAGTACGTCGGGGATAAC
PRIMER_RIGHT_4_SEQUENCE=GTGAGGATAGGTGTGAGCTGGT
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=262,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=60.574
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_02324 Ps_contig_mira-and-tgicl-ass_franssen_20267
SEQUENCE=ACGCTTCCATGGGCGATGGGAACACCATCAGCGGTCCATAGATGAAAAAGTTCCTCCTGTGTTGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAGTAAGAATATTGGTTTGCTGAAATTTCAGATGGGAAACTGAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCATTAGGGACCTCATTGTTTTTCTTATGAGTGTGTTTTGGTTC
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1662
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.154023
PRIMER_LEFT_SEQUENCE=GAAAAAGTTCCTCCTGTGTTGG
PRIMER_RIGHT_SEQUENCE=TCTCTCACTCTTGCATTAGCCA
PRIMER_LEFT=43,22
PRIMER_RIGHT=186,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.154
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=0.1710
PRIMER_LEFT_1_PENALTY=0.016950
PRIMER_RIGHT_1_PENALTY=0.154023
PRIMER_LEFT_1_SEQUENCE=GTGTTGGTATGGATCCTTTGGT
PRIMER_RIGHT_1_SEQUENCE=TCTCTCACTCTTGCATTAGCCA
PRIMER_LEFT_1=58,22
PRIMER_RIGHT_1=186,22
PRIMER_LEFT_1_TM=59.983
PRIMER_RIGHT_1_TM=60.154
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.1728
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=0.160609
PRIMER_LEFT_2_SEQUENCE=GAAAAAGTTCCTCCTGTGTTGG
PRIMER_RIGHT_2_SEQUENCE=ATGGCTTCTGCTTCTCTCACTC
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=198,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.161
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.1776
PRIMER_LEFT_3_PENALTY=0.016950
PRIMER_RIGHT_3_PENALTY=0.160609
PRIMER_LEFT_3_SEQUENCE=GTGTTGGTATGGATCCTTTGGT
PRIMER_RIGHT_3_SEQUENCE=ATGGCTTCTGCTTCTCTCACTC
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=198,22
PRIMER_LEFT_3_TM=59.983
PRIMER_RIGHT_3_TM=60.161
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=141
PRIMER_PAIR_PENALTY_4=0.3410
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=0.328860
PRIMER_LEFT_4_SEQUENCE=GAAAAAGTTCCTCCTGTGTTGG
PRIMER_RIGHT_4_SEQUENCE=AATGAGGTCCCTAATGGCTTCT
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=211,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=60.329
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=169
=
PRIMER_SEQUENCE_ID=SSR_02325 Ps_contig_mira-and-tgicl-ass_franssen_20268
SEQUENCE=ATGATKGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAGCTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGAAATTTCAGATGGGAAACTGAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCATTAGGGACCTCATTGTKTTTCTTMTGAGTGTGTTTTGGTTATGYCCCCGAC
TARGET=77,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02326 Ps_contig_mira-and-tgicl-ass_franssen_20268
SEQUENCE=ATGATKGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAGCTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGAAATTTCAGATGGGAAACTGAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCATTAGGGACCTCATTGTKTTTCTTMTGAGTGTGTTTTGGTTATGYCCCCGAC
TARGET=133,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02327 Ps_contig_mira-and-tgicl-ass_franssen_20268
SEQUENCE=ATGATKGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAGCTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGAAATTTCAGATGGGAAACTGAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCATTAGGGACCTCATTGTKTTTCTTMTGAGTGTGTTTTGGTTATGYCCCCGAC
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02328 Ps_contig_mira-and-tgicl-ass_franssen_20268
SEQUENCE=ATGATKGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAGCTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGAAATTTCAGATGGGAAACTGAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCATTAGGGACCTCATTGTKTTTCTTMTGAGTGTGTTTTGGTTATGYCCCCGAC
TARGET=235,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02329 Ps_contig_mira-and-tgicl-ass_franssen_20268
SEQUENCE=ATGATKGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAGCTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGAAATTTCAGATGGGAAACTGAAAAAGAGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCATTAGGGACCTCATTGTKTTTCTTMTGAGTGTGTTTTGGTTATGYCCCCGAC
TARGET=373,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02330 Ps_contig_mira-and-tgicl-ass_franssen_20270
SEQUENCE=CGAGTGCCTTACAGTTGTGGCTAATACAAATAAAAACAACAAATTAAATAAATAAAAGACAACCCACTCAACAATATTAATCAAAGAACTGACGAAATATATATGTGAAGAATCCCCTCGGTATTAGTTGTCCATGGCTTTCTCTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGKWSRYKRTGGWGGTGGRSACTATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCAC
TARGET=45,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02331 Ps_contig_mira-and-tgicl-ass_franssen_20270
SEQUENCE=CGAGTGCCTTACAGTTGTGGCTAATACAAATAAAAACAACAAATTAAATAAATAAAAGACAACCCACTCAACAATATTAATCAAAGAACTGACGAAATATATATGTGAAGAATCCCCTCGGTATTAGTTGTCCATGGCTTTCTCTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGKWSRYKRTGGWGGTGGRSACTATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCAC
TARGET=263,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02332 Ps_contig_mira-and-tgicl-ass_franssen_20270
SEQUENCE=CGAGTGCCTTACAGTTGTGGCTAATACAAATAAAAACAACAAATTAAATAAATAAAAGACAACCCACTCAACAATATTAATCAAAGAACTGACGAAATATATATGTGAAGAATCCCCTCGGTATTAGTTGTCCATGGCTTTCTCTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGKWSRYKRTGGWGGTGGRSACTATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCAC
TARGET=319,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02333 Ps_contig_mira-and-tgicl-ass_franssen_20277
SEQUENCE=GACACCATGAAGAAGAAGAAGAATACAGTTTTTGGACGATCCTAACTCTAGAACACAACTGTGTCGAGCATCATTCCCAAAGTCTGAACAATGTTAGAAGACATTAAATTAGAAACGTGCTCTTCAAGATGCTTCTTAGCATCCTGCCTTCCTACATTAGCAATTGCAAGCTTTTCTGGGGGCAGCTCATCTTCCTCTTGCACTGCCTTGTTGTACTTGGTTGCTAAACTTAGCATCTCCTGAACAGTCTGTTCATTAGTTTTAGAATGTGAATCAAATCGTCTGAGTGTCAAACCATCGGTCCATTTCTTCTTATGAAGGTTAAGCAACATCTTCTCTTCAAGCTCGTTCTTCCTGTAATTGATTGCTATTGAGTAATAATGTCGGTTCAAGCCATGGATCAGAGCTTGGATAGATTGGTTTGTTCAGATGGCCAAGGTTAGAGGTTGTCTGTCGTGGTTCTTCGACCCAGCATCATTWGKTTGTGGATTAANCAGGACCGGAAAGCATCAATCCAACCCTACCTTTCGTACCCTTTTAAACCTA
TARGET=9,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02334 Ps_contig_mira-and-tgicl-ass_franssen_20281
SEQUENCE=AGGGCAAAGATATAGCTGAGGTGATTGCTTCTGGTAAGGAAAAGTTGGCATCAGTGCCTTCTGGTGGTGGTGGTGGCGTTAAGGAGATCATTCATGATGAGCTTTCTAATGAACTTCCAGTAAGGTCCAAATGGAACCATTGCAACGGAGTTATCGTAGGTTAACCGTCTGATAGCAGAGGTTTGGAACCTTGTATTGAAAGAAGTCGCTTCGTGAGTTTGAAGGAAGAGTTTGAAGAGATCAGGAGTGGAAACAACAACAGTAGGCATGGAGCCAAAGTAAAGAGAGTACAAAGGGCCATAACGTTCTCCAAGACGGATGAGAGAATGATGAAGAAGAGGATTATCCAAAAGATGGAAAGATRACGAATAAAAGGAAGACGAGGTTTAGGACATGGTGGATTAGGAAGATGGCGAAGTGCCTTTGATTTAGCAGTGGGTGTTTGTGCTAGGGTGGCTATTGGACAAGTGCTTCTCTCTGTCGCTGCAAGGATAGC
TARGET=62,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02335 Ps_contig_mira-and-tgicl-ass_franssen_20286
SEQUENCE=AATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACGGTGGTGCATAGACAGGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATTATTTGTATGGAGGAGGCGGTGGAGAGTGGATACTTATATGGTGGAGGAGGTGAATTGTAATAACCGGTGGAGGTGGTGACTTGTACTTGTAAACTGGTGGTGGAGGAGAAGGATATTTGTATGGCTTCTCTGACGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCGACCGGTGGTGGGTGGAGATGAATAGATTGTTAGTTTGTTTGCTTGAGAGTGGACAAAGGTGGAAGGAAGAAAGTATGTANAATANAATAGT
TARGET=124,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2451
PRIMER_LEFT_PENALTY=0.073900
PRIMER_RIGHT_PENALTY=0.171245
PRIMER_LEFT_SEQUENCE=TTGTAAACGGTGGTGCATAGAC
PRIMER_RIGHT_SEQUENCE=CGCCTCCTCCATACAAATAATC
PRIMER_LEFT=40,22
PRIMER_RIGHT=191,22
PRIMER_LEFT_TM=59.926
PRIMER_RIGHT_TM=59.829
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=0.4648
PRIMER_LEFT_1_PENALTY=0.073900
PRIMER_RIGHT_1_PENALTY=0.390906
PRIMER_LEFT_1_SEQUENCE=TTGTAAACGGTGGTGCATAGAC
PRIMER_RIGHT_1_SEQUENCE=CCTCCACCGGTTATTACAATTC
PRIMER_LEFT_1=40,22
PRIMER_RIGHT_1=246,22
PRIMER_LEFT_1_TM=59.926
PRIMER_RIGHT_1_TM=59.609
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.4747
PRIMER_LEFT_2_PENALTY=0.408753
PRIMER_RIGHT_2_PENALTY=0.065960
PRIMER_LEFT_2_SEQUENCE=TTTGTAAACGGTGGTGCATAGA
PRIMER_RIGHT_2_SEQUENCE=CAAGTACAAGTCACCACCTCCA
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=60.409
PRIMER_RIGHT_2_TM=60.066
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.5038
PRIMER_LEFT_3_PENALTY=0.437791
PRIMER_RIGHT_3_PENALTY=0.065960
PRIMER_LEFT_3_SEQUENCE=GTAAACGGTGGTGCATAGACAG
PRIMER_RIGHT_3_SEQUENCE=CAAGTACAAGTCACCACCTCCA
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=59.562
PRIMER_RIGHT_3_TM=60.066
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.5765
PRIMER_LEFT_4_PENALTY=0.073900
PRIMER_RIGHT_4_PENALTY=0.502561
PRIMER_LEFT_4_SEQUENCE=TTGTAAACGGTGGTGCATAGAC
PRIMER_RIGHT_4_SEQUENCE=CCGTCAGAGAAGCCATACAAAT
PRIMER_LEFT_4=40,22
PRIMER_RIGHT_4=309,22
PRIMER_LEFT_4_TM=59.926
PRIMER_RIGHT_4_TM=60.503
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_02336 Ps_contig_mira-and-tgicl-ass_franssen_20286
SEQUENCE=AATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACGGTGGTGCATAGACAGGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATTATTTGTATGGAGGAGGCGGTGGAGAGTGGATACTTATATGGTGGAGGAGGTGAATTGTAATAACCGGTGGAGGTGGTGACTTGTACTTGTAAACTGGTGGTGGAGGAGAAGGATATTTGTATGGCTTCTCTGACGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCGACCGGTGGTGGGTGGAGATGAATAGATTGTTAGTTTGTTTGCTTGAGAGTGGACAAAGGTGGAAGGAAGAAAGTATGTANAATANAATAGT
TARGET=155,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2451
PRIMER_LEFT_PENALTY=0.073900
PRIMER_RIGHT_PENALTY=0.171245
PRIMER_LEFT_SEQUENCE=TTGTAAACGGTGGTGCATAGAC
PRIMER_RIGHT_SEQUENCE=CGCCTCCTCCATACAAATAATC
PRIMER_LEFT=40,22
PRIMER_RIGHT=191,22
PRIMER_LEFT_TM=59.926
PRIMER_RIGHT_TM=59.829
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=0.4648
PRIMER_LEFT_1_PENALTY=0.073900
PRIMER_RIGHT_1_PENALTY=0.390906
PRIMER_LEFT_1_SEQUENCE=TTGTAAACGGTGGTGCATAGAC
PRIMER_RIGHT_1_SEQUENCE=CCTCCACCGGTTATTACAATTC
PRIMER_LEFT_1=40,22
PRIMER_RIGHT_1=246,22
PRIMER_LEFT_1_TM=59.926
PRIMER_RIGHT_1_TM=59.609
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.4747
PRIMER_LEFT_2_PENALTY=0.408753
PRIMER_RIGHT_2_PENALTY=0.065960
PRIMER_LEFT_2_SEQUENCE=TTTGTAAACGGTGGTGCATAGA
PRIMER_RIGHT_2_SEQUENCE=CAAGTACAAGTCACCACCTCCA
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=60.409
PRIMER_RIGHT_2_TM=60.066
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.5038
PRIMER_LEFT_3_PENALTY=0.437791
PRIMER_RIGHT_3_PENALTY=0.065960
PRIMER_LEFT_3_SEQUENCE=GTAAACGGTGGTGCATAGACAG
PRIMER_RIGHT_3_SEQUENCE=CAAGTACAAGTCACCACCTCCA
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=59.562
PRIMER_RIGHT_3_TM=60.066
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.5765
PRIMER_LEFT_4_PENALTY=0.073900
PRIMER_RIGHT_4_PENALTY=0.502561
PRIMER_LEFT_4_SEQUENCE=TTGTAAACGGTGGTGCATAGAC
PRIMER_RIGHT_4_SEQUENCE=CCGTCAGAGAAGCCATACAAAT
PRIMER_LEFT_4=40,22
PRIMER_RIGHT_4=309,22
PRIMER_LEFT_4_TM=59.926
PRIMER_RIGHT_4_TM=60.503
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_02337 Ps_contig_mira-and-tgicl-ass_franssen_20286
SEQUENCE=AATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACGGTGGTGCATAGACAGGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATTATTTGTATGGAGGAGGCGGTGGAGAGTGGATACTTATATGGTGGAGGAGGTGAATTGTAATAACCGGTGGAGGTGGTGACTTGTACTTGTAAACTGGTGGTGGAGGAGAAGGATATTTGTATGGCTTCTCTGACGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCGACCGGTGGTGGGTGGAGATGAATAGATTGTTAGTTTGTTTGCTTGAGAGTGGACAAAGGTGGAAGGAAGAAAGTATGTANAATANAATAGT
TARGET=309,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3496
PRIMER_LEFT_PENALTY=0.343977
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=GGTGAATTGTAATAACCGGTGG
PRIMER_RIGHT_SEQUENCE=CACTCACCTCCTCCACCTTATC
PRIMER_LEFT=222,22
PRIMER_RIGHT=351,22
PRIMER_LEFT_TM=60.344
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=0.3496
PRIMER_LEFT_1_PENALTY=0.343977
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=GGTGAATTGTAATAACCGGTGG
PRIMER_RIGHT_1_SEQUENCE=ACTCACCTCCTCCACCTTATCA
PRIMER_LEFT_1=222,22
PRIMER_RIGHT_1=350,22
PRIMER_LEFT_1_TM=60.344
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.6443
PRIMER_LEFT_2_PENALTY=0.065960
PRIMER_RIGHT_2_PENALTY=0.578368
PRIMER_LEFT_2_SEQUENCE=TGGAGGTGGTGACTTGTACTTG
PRIMER_RIGHT_2_SEQUENCE=ATCTATTCATCTCCACCCACCA
PRIMER_LEFT_2=241,22
PRIMER_RIGHT_2=380,22
PRIMER_LEFT_2_TM=60.066
PRIMER_RIGHT_2_TM=60.578
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=0.7455
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.744773
PRIMER_LEFT_3_SEQUENCE=ATTGTAATAACCGGTGGAGGTG
PRIMER_RIGHT_3_SEQUENCE=CTTTCTTCCTTCCACCTTTGTC
PRIMER_LEFT_3=227,22
PRIMER_RIGHT_3=426,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=59.255
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=0.7496
PRIMER_LEFT_4_PENALTY=0.171245
PRIMER_RIGHT_4_PENALTY=0.578368
PRIMER_LEFT_4_SEQUENCE=GATTATTTGTATGGAGGAGGCG
PRIMER_RIGHT_4_SEQUENCE=ATCTATTCATCTCCACCCACCA
PRIMER_LEFT_4=170,22
PRIMER_RIGHT_4=380,22
PRIMER_LEFT_4_TM=59.829
PRIMER_RIGHT_4_TM=60.578
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_02338 Ps_contig_mira-and-tgicl-ass_franssen_20287
SEQUENCE=GACACAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAGACTTGCTTCTTTTTTCTGTTCTTACAYTCCTCTCAYTTCATGCAGTTGTCTCAGTTACACCGTTGCATTGTTCAACATCCACTTCGAACCCCTTTAAACCAAAGGARGTAGTTTCTAGCTTGTTACAAACCATAGTACCAAAAACAACTACCCCATCTACAAAACAA
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02339 Ps_contig_mira-and-tgicl-ass_franssen_20287
SEQUENCE=GACACAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAGACTTGCTTCTTTTTTCTGTTCTTACAYTCCTCTCAYTTCATGCAGTTGTCTCAGTTACACCGTTGCATTGTTCAACATCCACTTCGAACCCCTTTAAACCAAAGGARGTAGTTTCTAGCTTGTTACAAACCATAGTACCAAAAACAACTACCCCATCTACAAAACAA
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02340 Ps_contig_mira-and-tgicl-ass_franssen_20292
SEQUENCE=CAGGGTGAGGGATGAGGGTAGGTGTGCACTGGTGGTGGGGGAGATGAGTACTTGTACGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGGTACTTGTAAGGCTTCTTTGTGTGGTGTTGGTGGTGGAGGAGAGTGGTACACTGGGTGTGGGTGAGGATAGGTGTGAGCTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGAAATTTCAGATGGGAAACTGAAAAAGNGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCAAGTTACGGG
TARGET=68,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1593
PRIMER_LEFT_PENALTY=0.114470
PRIMER_RIGHT_PENALTY=0.044832
PRIMER_LEFT_SEQUENCE=GGGGAGATGAGTACTTGTACGG
PRIMER_RIGHT_SEQUENCE=ACACCCAGTGTACCACTCTCCT
PRIMER_LEFT=37,22
PRIMER_RIGHT=198,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=59.955
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=162
PRIMER_PAIR_PENALTY_1=0.6881
PRIMER_LEFT_1_PENALTY=0.114470
PRIMER_RIGHT_1_PENALTY=0.573649
PRIMER_LEFT_1_SEQUENCE=GGGGAGATGAGTACTTGTACGG
PRIMER_RIGHT_1_SEQUENCE=ACCAGCTCACACCTATCCTCAC
PRIMER_LEFT_1=37,22
PRIMER_RIGHT_1=222,22
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=60.574
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=0.8972
PRIMER_LEFT_2_PENALTY=0.114470
PRIMER_RIGHT_2_PENALTY=0.782778
PRIMER_LEFT_2_SEQUENCE=GGGGAGATGAGTACTTGTACGG
PRIMER_RIGHT_2_SEQUENCE=ATTCTTATTCCTCACCACCACC
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=304,22
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=59.217
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=1.1062
PRIMER_LEFT_3_PENALTY=0.114470
PRIMER_RIGHT_3_PENALTY=0.991761
PRIMER_LEFT_3_SEQUENCE=GGGGAGATGAGTACTTGTACGG
PRIMER_RIGHT_3_SEQUENCE=CCAGTGTACCACTCTCCTCCAC
PRIMER_LEFT_3=37,22
PRIMER_RIGHT_3=194,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=60.992
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=59.091
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=1.1802
PRIMER_LEFT_4_PENALTY=0.114470
PRIMER_RIGHT_4_PENALTY=1.065775
PRIMER_LEFT_4_SEQUENCE=GGGGAGATGAGTACTTGTACGG
PRIMER_RIGHT_4_SEQUENCE=ACCAACACCACACAAAGAAGC
PRIMER_LEFT_4=37,22
PRIMER_RIGHT_4=171,21
PRIMER_LEFT_4_TM=59.886
PRIMER_RIGHT_4_TM=60.066
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=135
=
PRIMER_SEQUENCE_ID=SSR_02341 Ps_contig_mira-and-tgicl-ass_franssen_20292
SEQUENCE=CAGGGTGAGGGATGAGGGTAGGTGTGCACTGGTGGTGGGGGAGATGAGTACTTGTACGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGGTACTTGTAAGGCTTCTTTGTGTGGTGTTGGTGGTGGAGGAGAGTGGTACACTGGGTGTGGGTGAGGATAGGTGTGAGCTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGAAATTTCAGATGGGAAACTGAAAAAGNGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCAAGTTACGGG
TARGET=120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1593
PRIMER_LEFT_PENALTY=0.114470
PRIMER_RIGHT_PENALTY=0.044832
PRIMER_LEFT_SEQUENCE=GGGGAGATGAGTACTTGTACGG
PRIMER_RIGHT_SEQUENCE=ACACCCAGTGTACCACTCTCCT
PRIMER_LEFT=37,22
PRIMER_RIGHT=198,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=59.955
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=162
PRIMER_PAIR_PENALTY_1=0.2600
PRIMER_LEFT_1_PENALTY=0.215215
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_LEFT_1_SEQUENCE=TACGGTTTCTTATGTGGTGGTG
PRIMER_RIGHT_1_SEQUENCE=ACACCCAGTGTACCACTCTCCT
PRIMER_LEFT_1=54,22
PRIMER_RIGHT_1=198,22
PRIMER_LEFT_1_TM=59.785
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=0.2600
PRIMER_LEFT_2_PENALTY=0.215215
PRIMER_RIGHT_2_PENALTY=0.044832
PRIMER_LEFT_2_SEQUENCE=TGTACGGTTTCTTATGTGGTGG
PRIMER_RIGHT_2_SEQUENCE=ACACCCAGTGTACCACTCTCCT
PRIMER_LEFT_2=52,22
PRIMER_RIGHT_2=198,22
PRIMER_LEFT_2_TM=59.785
PRIMER_RIGHT_2_TM=59.955
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=0.6059
PRIMER_LEFT_3_PENALTY=0.215215
PRIMER_RIGHT_3_PENALTY=0.390638
PRIMER_LEFT_3_SEQUENCE=TACGGTTTCTTATGTGGTGGTG
PRIMER_RIGHT_3_SEQUENCE=CNCTTTTTCAGTTTCCCATCTG
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=344,22
PRIMER_LEFT_3_TM=59.785
PRIMER_RIGHT_3_TM=60.391
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.6059
PRIMER_LEFT_4_PENALTY=0.215215
PRIMER_RIGHT_4_PENALTY=0.390638
PRIMER_LEFT_4_SEQUENCE=TGTACGGTTTCTTATGTGGTGG
PRIMER_RIGHT_4_SEQUENCE=CNCTTTTTCAGTTTCCCATCTG
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=344,22
PRIMER_LEFT_4_TM=59.785
PRIMER_RIGHT_4_TM=60.391
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_02342 Ps_contig_mira-and-tgicl-ass_franssen_20292
SEQUENCE=CAGGGTGAGGGATGAGGGTAGGTGTGCACTGGTGGTGGGGGAGATGAGTACTTGTACGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGGTACTTGTAAGGCTTCTTTGTGTGGTGTTGGTGGTGGAGGAGAGTGGTACACTGGGTGTGGGTGAGGATAGGTGTGAGCTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATCCTTTGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAGAATATTGGTTTGCTGAAATTTCAGATGGGAAACTGAAAAAGNGTATGGCTAATGCAAGAGTGAGAGAAGCAGAAGCCAAGTTACGGG
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3574
PRIMER_LEFT_PENALTY=0.203343
PRIMER_RIGHT_PENALTY=0.154023
PRIMER_LEFT_SEQUENCE=TGTAAGGCTTCTTTGTGTGGTG
PRIMER_RIGHT_SEQUENCE=TCTCTCACTCTTGCATTAGCCA
PRIMER_LEFT=145,22
PRIMER_RIGHT=368,22
PRIMER_LEFT_TM=60.203
PRIMER_RIGHT_TM=60.154
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.3991
PRIMER_LEFT_1_PENALTY=0.203343
PRIMER_RIGHT_1_PENALTY=0.195770
PRIMER_LEFT_1_SEQUENCE=TGTAAGGCTTCTTTGTGTGGTG
PRIMER_RIGHT_1_SEQUENCE=ACTTGGCTTCTGCTTCTCTCAC
PRIMER_LEFT_1=145,22
PRIMER_RIGHT_1=382,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=60.196
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.5260
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.322707
PRIMER_LEFT_2_SEQUENCE=TGTAAGGCTTCTTTGTGTGGTG
PRIMER_RIGHT_2_SEQUENCE=CTTGGCTTCTGCTTCTCTCACT
PRIMER_LEFT_2=145,22
PRIMER_RIGHT_2=381,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=60.323
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=0.5940
PRIMER_LEFT_3_PENALTY=0.203343
PRIMER_RIGHT_3_PENALTY=0.390638
PRIMER_LEFT_3_SEQUENCE=TGTAAGGCTTCTTTGTGTGGTG
PRIMER_RIGHT_3_SEQUENCE=CNCTTTTTCAGTTTCCCATCTG
PRIMER_LEFT_3=145,22
PRIMER_RIGHT_3=344,22
PRIMER_LEFT_3_TM=60.203
PRIMER_RIGHT_3_TM=60.391
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=0.6059
PRIMER_LEFT_4_PENALTY=0.215215
PRIMER_RIGHT_4_PENALTY=0.390638
PRIMER_LEFT_4_SEQUENCE=TACGGTTTCTTATGTGGTGGTG
PRIMER_RIGHT_4_SEQUENCE=CNCTTTTTCAGTTTCCCATCTG
PRIMER_LEFT_4=54,22
PRIMER_RIGHT_4=344,22
PRIMER_LEFT_4_TM=59.785
PRIMER_RIGHT_4_TM=60.391
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_02343 Ps_contig_mira-and-tgicl-ass_franssen_20297
SEQUENCE=TGTKTTGGTAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGACAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATTACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTAATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATTAATTGGGTTGTAGATTACAGTATCTCTTATGTGTTGTGATTCTTTTTTTACTGNGACACTT
TARGET=163,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02344 Ps_contig_mira-and-tgicl-ass_franssen_20306
SEQUENCE=AAAAATAACAAATACAGGAAAGTGTGGAATCGCAATGGATGCTACATACCCAACTAAGCTTCGTAAAAAACTCTGAAGTTACTAATTCTGGTTATGAAAAAAACTCAAATGCTTGTACCAGTGCTTGAAACACCTACATACGTTGTNTTATNTCGTACCTTTAGGTTAGGTAATTAAAGTAAATTAATTAGTTCGTTACTTAAGTAAGTTTAATTTTTTAACTTTACCTTTTAACTTTTTAAAATTAACTCGTTTTTACGTTTTTTAACTTAACTTTCGTAGGTTGGTTACGTTACCTTCCTTACTTAATTAGTTAGTTAGTTAACGTAACCTACGTAAGGTTCGTTAACGTTTTAAAAAAAAGGTTT
TARGET=310,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02345 Ps_contig_mira-and-tgicl-ass_franssen_20311
SEQUENCE=GACACAAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAGACTTGCTTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGAACCCTTTAAACCAAAAGAAGTAGTTTTCAGCTTGTTACCAAACCATACGTACCAAAAACAAACTACCCCATCTACAAAASAAAAGGGGG
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02346 Ps_contig_mira-and-tgicl-ass_franssen_20311
SEQUENCE=GACACAAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAGACTTGCTTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGAACCCTTTAAACCAAAAGAAGTAGTTTTCAGCTTGTTACCAAACCATACGTACCAAAAACAAACTACCCCATCTACAAAASAAAAGGGGG
TARGET=59,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02347 Ps_contig_mira-and-tgicl-ass_franssen_20315
SEQUENCE=ATGTTGTTCGTTTATGTTGTTTTAATTTGTCTTTTCTTGTCCCTTTTATGTTTGAGATAGTATATGAGGAATCTGATATTGATGATGAGTTTGATGAAGAGTATGATTGGGATGATGATGATGATGATGGCGGCTATGAAAGTTGCGAGAATTATGGAGATGAGTGAAGTATGTGATGAAAACAAGTAAACAGAAGATGCTTTCTTACAAATGACAA
TARGET=111,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02348 Ps_contig_mira-and-tgicl-ass_franssen_20320
SEQUENCE=ACTYATATTATACATATACGAGCATTAACTTAGTTAGGTTAGATACTAGTTTTACTACGTTACAAGTTCCTATATGTTAATTTAAAACAAAACAAAACATTATTATTAGCAACTCTGGAAGAAACTAAAAAAAACAACATGACTAAACTTATTT
TARGET=84,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02349 Ps_contig_mira-and-tgicl-ass_franssen_20348
SEQUENCE=GAACAACAAAACCTATTTTGAGAAAACATGGGGTCTCAAATGACCTCTATTACTCTCACTATTGCATTGACAATACTCTCTCTCACCTTGCCATCTCAAGCAAACAATTACCTCTATTCATCTCCACCACCACCACCAAAACCTTACTACTACCAATCTCCACCACCACCGGTGCACTCACCTCCTCCTCCTTACCACTACTAGATAGTGGCTTACCGTAGTATCCCATGGTA
TARGET=124,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1826
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.087031
PRIMER_LEFT_SEQUENCE=AAAACATGGGGTCTCAAATGAC
PRIMER_RIGHT_SEQUENCE=TGGGATACTACGGTAAGCCACT
PRIMER_LEFT=22,22
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.2604
PRIMER_LEFT_1_PENALTY=0.173354
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=TGGGGTCTCAAATGACCTCTAT
PRIMER_RIGHT_1_SEQUENCE=TGGGATACTACGGTAAGCCACT
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=227,22
PRIMER_LEFT_1_TM=59.827
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.2604
PRIMER_LEFT_2_PENALTY=0.173354
PRIMER_RIGHT_2_PENALTY=0.087031
PRIMER_LEFT_2_SEQUENCE=ATGGGGTCTCAAATGACCTCTA
PRIMER_RIGHT_2_SEQUENCE=TGGGATACTACGGTAAGCCACT
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=59.827
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.3080
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.087031
PRIMER_LEFT_3_SEQUENCE=AGAAAACATGGGGTCTCAAATG
PRIMER_RIGHT_3_SEQUENCE=TGGGATACTACGGTAAGCCACT
PRIMER_LEFT_3=20,22
PRIMER_RIGHT_3=227,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.3761
PRIMER_LEFT_4_PENALTY=0.289100
PRIMER_RIGHT_4_PENALTY=0.087031
PRIMER_LEFT_4_SEQUENCE=CCTATTTTGAGAAAACATGGGG
PRIMER_RIGHT_4_SEQUENCE=TGGGATACTACGGTAAGCCACT
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=59.711
PRIMER_RIGHT_4_TM=59.913
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_02350 Ps_contig_mira-and-tgicl-ass_franssen_20348
SEQUENCE=GAACAACAAAACCTATTTTGAGAAAACATGGGGTCTCAAATGACCTCTATTACTCTCACTATTGCATTGACAATACTCTCTCTCACCTTGCCATCTCAAGCAAACAATTACCTCTATTCATCTCCACCACCACCACCAAAACCTTACTACTACCAATCTCCACCACCACCGGTGCACTCACCTCCTCCTCCTTACCACTACTAGATAGTGGCTTACCGTAGTATCCCATGGTA
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1826
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.087031
PRIMER_LEFT_SEQUENCE=AAAACATGGGGTCTCAAATGAC
PRIMER_RIGHT_SEQUENCE=TGGGATACTACGGTAAGCCACT
PRIMER_LEFT=22,22
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.2604
PRIMER_LEFT_1_PENALTY=0.173354
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=TGGGGTCTCAAATGACCTCTAT
PRIMER_RIGHT_1_SEQUENCE=TGGGATACTACGGTAAGCCACT
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=227,22
PRIMER_LEFT_1_TM=59.827
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.2604
PRIMER_LEFT_2_PENALTY=0.173354
PRIMER_RIGHT_2_PENALTY=0.087031
PRIMER_LEFT_2_SEQUENCE=ATGGGGTCTCAAATGACCTCTA
PRIMER_RIGHT_2_SEQUENCE=TGGGATACTACGGTAAGCCACT
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=59.827
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.3080
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.087031
PRIMER_LEFT_3_SEQUENCE=AGAAAACATGGGGTCTCAAATG
PRIMER_RIGHT_3_SEQUENCE=TGGGATACTACGGTAAGCCACT
PRIMER_LEFT_3=20,22
PRIMER_RIGHT_3=227,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.3761
PRIMER_LEFT_4_PENALTY=0.289100
PRIMER_RIGHT_4_PENALTY=0.087031
PRIMER_LEFT_4_SEQUENCE=CCTATTTTGAGAAAACATGGGG
PRIMER_RIGHT_4_SEQUENCE=TGGGATACTACGGTAAGCCACT
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=59.711
PRIMER_RIGHT_4_TM=59.913
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_02351 Ps_contig_mira-and-tgicl-ass_franssen_20349
SEQUENCE=GTCGGGGGTACTAATATTATTCATTCATTCTTCACACTATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCYTCACACCACCACTCTCAACTACCAAAAACATGGAAACAACCCTCCACTCTATCTTTCATCTCTCTCAAACCAACCAATTCACACCAAGAACAAACAAAAATTAGCGTACGACCAAAACCGAAAACGGAAAAAACGGGAAAAACCCCGTAAAAAAAAACCCCCGGGTAAACGTTTA
TARGET=19,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02352 Ps_contig_mira-and-tgicl-ass_franssen_20349
SEQUENCE=GTCGGGGGTACTAATATTATTCATTCATTCTTCACACTATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCYTCACACCACCACTCTCAACTACCAAAAACATGGAAACAACCCTCCACTCTATCTTTCATCTCTCTCAAACCAACCAATTCACACCAAGAACAAACAAAAATTAGCGTACGACCAAAACCGAAAACGGAAAAAACGGGAAAAACCCCGTAAAAAAAAACCCCCGGGTAAACGTTTA
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02353 Ps_contig_mira-and-tgicl-ass_franssen_20354
SEQUENCE=TCCTTTAAAACCTTACCAAACCTTAGTACAACTACTTCCGGTACGGCNGGTTTTGGTATGAAAACAAAGTAACATGCTGGAAGATCTATCCAACTTAAGACTTGTTGAAGGTGATTCTGCTGGAACTGTTACCGCTTTCTATATGTCATCGGAAGGTACAAAGCACAACGAGTTTGATTTTGAGTTCTTAGGAAACAGCACAGGTGAACCTTATTCTGTTCAAACAAATGTGTATGTTAATGGAGTTGGTAACCGTGAACAAAGACTTAACCTTTGGTTTGATCCTACTAAGGATTTTCACTCTTACTCCATTTTCTGGTATCAACGCAGAGTTGTATTTTTGGTGGATGAAACACCAATAAGAGTACACACAAACATGGAACATAGAGGAATTCCATATCCAAAAGACCAAGCAATGGGAGTATACAGTTCAATATGGAATGCAGATGATTGGGCAACACAAGGAGGAAGAGTGAAAACAGATTGGAGTCATGCACCTTTCCTTGTAACCTATAAATCCTTTGAAATCAATGCTTGCGAGTGTCCGGTATCGGTGGCTGGAATGGATAATAGAAAGAGATGTAGCAGTAGTGAAGATAAGAAATATTGGTGGGATGAACCAAAACTGTCAGAGCTTAATTTGCATCAGAGTCATCAACTAATGTGGGTTAGGGCTAAACATATGGTTTATGATTATTGTAATGATGCTTCTAGGTTTCCTGTTACTCCTCTTGAGTGTCTTCATCATCGTCATAGATTATTCTAATCACGTCAAACTTGTGTATTATTATTATGGCTGGAAATGGTGAATGTTGGTGTAAGAAATGTTCCACTTGTGTCATATTATTGTATGATATTATTGGCAAACTATATATAATGTGGAGAGAAAGTGTTAAACAAGGTGATGTCAAACTTGTGTATTATTATGGGG
TARGET=783,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5159
PRIMER_LEFT_PENALTY=0.107763
PRIMER_RIGHT_PENALTY=0.408180
PRIMER_LEFT_SEQUENCE=TCAACTAATGTGGGTTAGGGCT
PRIMER_RIGHT_SEQUENCE=TACACCAACATTCACCATTTCC
PRIMER_LEFT=654,22
PRIMER_RIGHT=819,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=59.592
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=1.1405
PRIMER_LEFT_1_PENALTY=0.732310
PRIMER_RIGHT_1_PENALTY=0.408180
PRIMER_LEFT_1_SEQUENCE=ATCAACTAATGTGGGTTAGGGC
PRIMER_RIGHT_1_SEQUENCE=TACACCAACATTCACCATTTCC
PRIMER_LEFT_1=653,22
PRIMER_RIGHT_1=819,22
PRIMER_LEFT_1_TM=59.268
PRIMER_RIGHT_1_TM=59.592
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=1.1910
PRIMER_LEFT_2_PENALTY=0.782819
PRIMER_RIGHT_2_PENALTY=0.408180
PRIMER_LEFT_2_SEQUENCE=GAAATATTGGTGGGATGAACCA
PRIMER_RIGHT_2_SEQUENCE=TACACCAACATTCACCATTTCC
PRIMER_LEFT_2=600,22
PRIMER_RIGHT_2=819,22
PRIMER_LEFT_2_TM=60.783
PRIMER_RIGHT_2_TM=59.592
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=1.2197
PRIMER_LEFT_3_PENALTY=0.811488
PRIMER_RIGHT_3_PENALTY=0.408180
PRIMER_LEFT_3_SEQUENCE=TATTGGTGGGATGAACCAAAAC
PRIMER_RIGHT_3_SEQUENCE=TACACCAACATTCACCATTTCC
PRIMER_LEFT_3=604,22
PRIMER_RIGHT_3=819,22
PRIMER_LEFT_3_TM=60.811
PRIMER_RIGHT_3_TM=59.592
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=216
PRIMER_PAIR_PENALTY_4=1.3367
PRIMER_LEFT_4_PENALTY=0.928475
PRIMER_RIGHT_4_PENALTY=0.408180
PRIMER_LEFT_4_SEQUENCE=AGAAATATTGGTGGGATGAACC
PRIMER_RIGHT_4_SEQUENCE=TACACCAACATTCACCATTTCC
PRIMER_LEFT_4=599,22
PRIMER_RIGHT_4=819,22
PRIMER_LEFT_4_TM=59.072
PRIMER_RIGHT_4_TM=59.592
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_02354 Ps_contig_mira-and-tgicl-ass_franssen_20361
SEQUENCE=AAACTAGACGGAAACGTAAATAATTATAGTGTAGGAAAGTAACCCTCCCGGTACGTTAGTTTAGGTTTCGTATGGNTTCTCTGCTTTCACACTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTTTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGACGGCAACAGAGGTCAACAGGCTGTGAATGTTGATAAAAGTTCTTTGAATAGTCAGCTTCAYATGCAAGATTGGTACCAAAATACTGCATCCATCAAATTGGTGATATATTCTGCATCCTTCAAATTGGTGATATATACCGCATTGTCAGCAATGAAAAGACTAGGGCAACTTGTCCTTCTTGGATCTCACTAGTGGTCTTTAGTTATTTTTTTCTTGTTATTTCAGACTAAATTAATTATTTTAGTAAACTAAATTGTTTACG
TARGET=199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02355 Ps_contig_mira-and-tgicl-ass_franssen_20361
SEQUENCE=AAACTAGACGGAAACGTAAATAATTATAGTGTAGGAAAGTAACCCTCCCGGTACGTTAGTTTAGGTTTCGTATGGNTTCTCTGCTTTCACACTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTTTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGACGGCAACAGAGGTCAACAGGCTGTGAATGTTGATAAAAGTTCTTTGAATAGTCAGCTTCAYATGCAAGATTGGTACCAAAATACTGCATCCATCAAATTGGTGATATATTCTGCATCCTTCAAATTGGTGATATATACCGCATTGTCAGCAATGAAAAGACTAGGGCAACTTGTCCTTCTTGGATCTCACTAGTGGTCTTTAGTTATTTTTTTCTTGTTATTTCAGACTAAATTAATTATTTTAGTAAACTAAATTGTTTACG
TARGET=255,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02356 Ps_contig_mira-and-tgicl-ass_franssen_20361
SEQUENCE=AAACTAGACGGAAACGTAAATAATTATAGTGTAGGAAAGTAACCCTCCCGGTACGTTAGTTTAGGTTTCGTATGGNTTCTCTGCTTTCACACTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTTTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGACGGCAACAGAGGTCAACAGGCTGTGAATGTTGATAAAAGTTCTTTGAATAGTCAGCTTCAYATGCAAGATTGGTACCAAAATACTGCATCCATCAAATTGGTGATATATTCTGCATCCTTCAAATTGGTGATATATACCGCATTGTCAGCAATGAAAAGACTAGGGCAACTTGTCCTTCTTGGATCTCACTAGTGGTCTTTAGTTATTTTTTTCTTGTTATTTCAGACTAAATTAATTATTTTAGTAAACTAAATTGTTTACG
TARGET=303,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02357 Ps_contig_mira-and-tgicl-ass_franssen_20362
SEQUENCE=AAACCCCCGGGTTACCCCGTAACCCTTACCTACCCAACCCGACCCCACCCTAAAACGGAATCCATACATTACTCANCTCCTCCACCTCCACCACGCTCCACCACCTATCCTCACCCACCACCCACGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTTTACTCGCCACCACCCATTTACAAATATAAGTCTCCTCCTCTACCTGTTTACT
TARGET=238,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1955
PRIMER_LEFT_PENALTY=0.044832
PRIMER_RIGHT_PENALTY=0.150698
PRIMER_LEFT_SEQUENCE=ACACCCAGTGTACCACTCTCCT
PRIMER_RIGHT_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT=170,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=59.955
PRIMER_RIGHT_TM=59.849
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.3540
PRIMER_LEFT_1_PENALTY=0.203343
PRIMER_RIGHT_1_PENALTY=0.150698
PRIMER_LEFT_1_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_RIGHT_1_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT_1=202,22
PRIMER_RIGHT_1=371,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=59.849
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.3540
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.150698
PRIMER_LEFT_2_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_RIGHT_2_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT_2=199,22
PRIMER_RIGHT_2=371,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=59.849
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.3657
PRIMER_LEFT_3_PENALTY=0.118974
PRIMER_RIGHT_3_PENALTY=0.246726
PRIMER_LEFT_3_SEQUENCE=GTAACCCTTACCTACCCAACCC
PRIMER_RIGHT_3_SEQUENCE=AATGGTAGACAGGGTGAGGATG
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=285,22
PRIMER_LEFT_3_TM=59.881
PRIMER_RIGHT_3_TM=60.247
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.4485
PRIMER_LEFT_4_PENALTY=0.297793
PRIMER_RIGHT_4_PENALTY=0.150698
PRIMER_LEFT_4_SEQUENCE=ACACCACACAAGAAGCCTTACA
PRIMER_RIGHT_4_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT_4=201,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=59.702
PRIMER_RIGHT_4_TM=59.849
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_02358 Ps_contig_mira-and-tgicl-ass_franssen_20362
SEQUENCE=AAACCCCCGGGTTACCCCGTAACCCTTACCTACCCAACCCGACCCCACCCTAAAACGGAATCCATACATTACTCANCTCCTCCACCTCCACCACGCTCCACCACCTATCCTCACCCACCACCCACGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTTTACTCGCCACCACCCATTTACAAATATAAGTCTCCTCCTCTACCTGTTTACT
TARGET=289,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1955
PRIMER_LEFT_PENALTY=0.044832
PRIMER_RIGHT_PENALTY=0.150698
PRIMER_LEFT_SEQUENCE=ACACCCAGTGTACCACTCTCCT
PRIMER_RIGHT_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT=170,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=59.955
PRIMER_RIGHT_TM=59.849
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.3540
PRIMER_LEFT_1_PENALTY=0.203343
PRIMER_RIGHT_1_PENALTY=0.150698
PRIMER_LEFT_1_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_RIGHT_1_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT_1=202,22
PRIMER_RIGHT_1=371,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=59.849
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.3540
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.150698
PRIMER_LEFT_2_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_RIGHT_2_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT_2=199,22
PRIMER_RIGHT_2=371,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=59.849
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.3974
PRIMER_LEFT_3_PENALTY=0.246726
PRIMER_RIGHT_3_PENALTY=0.150698
PRIMER_LEFT_3_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_3_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT_3=264,22
PRIMER_RIGHT_3=371,22
PRIMER_LEFT_3_TM=60.247
PRIMER_RIGHT_3_TM=59.849
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=108
PRIMER_PAIR_PENALTY_4=0.4485
PRIMER_LEFT_4_PENALTY=0.297793
PRIMER_RIGHT_4_PENALTY=0.150698
PRIMER_LEFT_4_SEQUENCE=ACACCACACAAGAAGCCTTACA
PRIMER_RIGHT_4_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT_4=201,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=59.702
PRIMER_RIGHT_4_TM=59.849
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_02359 Ps_contig_mira-and-tgicl-ass_franssen_20362
SEQUENCE=AAACCCCCGGGTTACCCCGTAACCCTTACCTACCCAACCCGACCCCACCCTAAAACGGAATCCATACATTACTCANCTCCTCCACCTCCACCACGCTCCACCACCTATCCTCACCCACCACCCACGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTTTACTCGCCACCACCCATTTACAAATATAAGTCTCCTCCTCTACCTGTTTACT
TARGET=331,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1955
PRIMER_LEFT_PENALTY=0.044832
PRIMER_RIGHT_PENALTY=0.150698
PRIMER_LEFT_SEQUENCE=ACACCCAGTGTACCACTCTCCT
PRIMER_RIGHT_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT=170,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=59.955
PRIMER_RIGHT_TM=59.849
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.3540
PRIMER_LEFT_1_PENALTY=0.203343
PRIMER_RIGHT_1_PENALTY=0.150698
PRIMER_LEFT_1_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_RIGHT_1_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT_1=202,22
PRIMER_RIGHT_1=371,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=59.849
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.3540
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.150698
PRIMER_LEFT_2_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_RIGHT_2_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT_2=199,22
PRIMER_RIGHT_2=371,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=59.849
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.3974
PRIMER_LEFT_3_PENALTY=0.246726
PRIMER_RIGHT_3_PENALTY=0.150698
PRIMER_LEFT_3_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_3_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT_3=264,22
PRIMER_RIGHT_3=371,22
PRIMER_LEFT_3_TM=60.247
PRIMER_RIGHT_3_TM=59.849
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=108
PRIMER_PAIR_PENALTY_4=0.4485
PRIMER_LEFT_4_PENALTY=0.297793
PRIMER_RIGHT_4_PENALTY=0.150698
PRIMER_LEFT_4_SEQUENCE=ACACCACACAAGAAGCCTTACA
PRIMER_RIGHT_4_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_LEFT_4=201,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=59.702
PRIMER_RIGHT_4_TM=59.849
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_02360 Ps_contig_mira-and-tgicl-ass_franssen_20363
SEQUENCE=CGAAACTAAGTTTAAGGGTTAACCGGAAACCGGAAAGTTACTTAACGTAAACGTTAAACGGAAAAGGGGAAAAGGTAACCCTTCCCCTACCGAACCGAACCGACGACGACGGTCCGTTCCTACTTTTTTAGTTTTTCGTTCGGTCGGAACGAACGAACCTAAACGTAAGGGAAGGTAGGGTCGGGTACCCCGTTTACCGTTCNANTACAATATACAGAAGGAATCAACTCTCCATTTTGGTGCTCCGTCTCCGTGGTGGGATGCAAATCTTTGTCAAAACTCTCACTGGAAAGACCATTACCCTTGAGGTGGAGAGCTCTGATACCATTGACAATGTCAAAGCCAAGATTCAGGATAAAGAGGGCATTCCACCTGACCAACAGAGGCTCATCTTTGCTGGCAAACAACTTGAGGATGGCCGTACTCTCGCTGACTACAACATCCAGAAGGAGTCCACCCTTCATCTTGTCCTTCGTCTCCGTGGAGGTATGCAGATCTT
TARGET=89,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0727
PRIMER_LEFT_PENALTY=0.068375
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=CGTAAACGTTAAACGGAAAAGG
PRIMER_RIGHT_SEQUENCE=AGCACCAAAATGGAGAGTTGAT
PRIMER_LEFT=45,22
PRIMER_RIGHT=243,22
PRIMER_LEFT_TM=59.932
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.2044
PRIMER_LEFT_1_PENALTY=0.068375
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CGTAAACGTTAAACGGAAAAGG
PRIMER_RIGHT_1_SEQUENCE=GGGTAATGGTCTTTCCAGTGAG
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=302,22
PRIMER_LEFT_1_TM=59.932
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.2044
PRIMER_LEFT_2_PENALTY=0.068375
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CGTAAACGTTAAACGGAAAAGG
PRIMER_RIGHT_2_SEQUENCE=AGGGTAATGGTCTTTCCAGTGA
PRIMER_LEFT_2=45,22
PRIMER_RIGHT_2=303,22
PRIMER_LEFT_2_TM=59.932
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.2055
PRIMER_LEFT_3_PENALTY=0.201149
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=GTTAAACGGAAAAGGGGAAAAG
PRIMER_RIGHT_3_SEQUENCE=AGCACCAAAATGGAGAGTTGAT
PRIMER_LEFT_3=52,22
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=60.201
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=0.3372
PRIMER_LEFT_4_PENALTY=0.201149
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=GTTAAACGGAAAAGGGGAAAAG
PRIMER_RIGHT_4_SEQUENCE=GGGTAATGGTCTTTCCAGTGAG
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=302,22
PRIMER_LEFT_4_TM=60.201
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_02361 Ps_contig_mira-and-tgicl-ass_franssen_20363
SEQUENCE=CGAAACTAAGTTTAAGGGTTAACCGGAAACCGGAAAGTTACTTAACGTAAACGTTAAACGGAAAAGGGGAAAAGGTAACCCTTCCCCTACCGAACCGAACCGACGACGACGGTCCGTTCCTACTTTTTTAGTTTTTCGTTCGGTCGGAACGAACGAACCTAAACGTAAGGGAAGGTAGGGTCGGGTACCCCGTTTACCGTTCNANTACAATATACAGAAGGAATCAACTCTCCATTTTGGTGCTCCGTCTCCGTGGTGGGATGCAAATCTTTGTCAAAACTCTCACTGGAAAGACCATTACCCTTGAGGTGGAGAGCTCTGATACCATTGACAATGTCAAAGCCAAGATTCAGGATAAAGAGGGCATTCCACCTGACCAACAGAGGCTCATCTTTGCTGGCAAACAACTTGAGGATGGCCGTACTCTCGCTGACTACAACATCCAGAAGGAGTCCACCCTTCATCTTGTCCTTCGTCTCCGTGGAGGTATGCAGATCTT
TARGET=147,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0727
PRIMER_LEFT_PENALTY=0.068375
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=CGTAAACGTTAAACGGAAAAGG
PRIMER_RIGHT_SEQUENCE=AGCACCAAAATGGAGAGTTGAT
PRIMER_LEFT=45,22
PRIMER_RIGHT=243,22
PRIMER_LEFT_TM=59.932
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.2044
PRIMER_LEFT_1_PENALTY=0.068375
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CGTAAACGTTAAACGGAAAAGG
PRIMER_RIGHT_1_SEQUENCE=GGGTAATGGTCTTTCCAGTGAG
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=302,22
PRIMER_LEFT_1_TM=59.932
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.2044
PRIMER_LEFT_2_PENALTY=0.068375
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CGTAAACGTTAAACGGAAAAGG
PRIMER_RIGHT_2_SEQUENCE=AGGGTAATGGTCTTTCCAGTGA
PRIMER_LEFT_2=45,22
PRIMER_RIGHT_2=303,22
PRIMER_LEFT_2_TM=59.932
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.2055
PRIMER_LEFT_3_PENALTY=0.201149
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=GTTAAACGGAAAAGGGGAAAAG
PRIMER_RIGHT_3_SEQUENCE=AGCACCAAAATGGAGAGTTGAT
PRIMER_LEFT_3=52,22
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=60.201
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=0.3372
PRIMER_LEFT_4_PENALTY=0.201149
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=GTTAAACGGAAAAGGGGAAAAG
PRIMER_RIGHT_4_SEQUENCE=GGGTAATGGTCTTTCCAGTGAG
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=302,22
PRIMER_LEFT_4_TM=60.201
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_02362 Ps_contig_mira-and-tgicl-ass_franssen_20368
SEQUENCE=TAGGTGTGNACTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGTAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGTGGAGGAGAGTGGTACACTGGGTGTGGGTGAGGAGCACTACACAGAGATTGTGGATGACTTGCGTGGAGGTGATCCATGCATTGGTTCAGGATCTCAGGTGGCAAGCCAGGAGACATACGGAACTTTGGGNCCATTGNGAGGA
TARGET=12,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02363 Ps_contig_mira-and-tgicl-ass_franssen_20368
SEQUENCE=TAGGTGTGNACTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGTAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGTGGAGGAGAGTGGTACACTGGGTGTGGGTGAGGAGCACTACACAGAGATTGTGGATGACTTGCGTGGAGGTGATCCATGCATTGGTTCAGGATCTCAGGTGGCAAGCCAGGAGACATACGGAACTTTGGGNCCATTGNGAGGA
TARGET=63,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1902
PRIMER_LEFT_PENALTY=0.145354
PRIMER_RIGHT_PENALTY=0.044832
PRIMER_LEFT_SEQUENCE=GAGGATGAGGGTAGGTATGCAC
PRIMER_RIGHT_SEQUENCE=ACACCCAGTGTACCACTCTCCT
PRIMER_LEFT=40,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=59.855
PRIMER_RIGHT_TM=59.955
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.3487
PRIMER_LEFT_1_PENALTY=0.145354
PRIMER_RIGHT_1_PENALTY=0.203343
PRIMER_LEFT_1_SEQUENCE=GAGGATGAGGGTAGGTATGCAC
PRIMER_RIGHT_1_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_LEFT_1=40,22
PRIMER_RIGHT_1=202,22
PRIMER_LEFT_1_TM=59.855
PRIMER_RIGHT_1_TM=60.203
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.3487
PRIMER_LEFT_2_PENALTY=0.145354
PRIMER_RIGHT_2_PENALTY=0.203343
PRIMER_LEFT_2_SEQUENCE=GTGAGGATGAGGGTAGGTATGC
PRIMER_RIGHT_2_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=202,22
PRIMER_LEFT_2_TM=59.855
PRIMER_RIGHT_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.3487
PRIMER_LEFT_3_PENALTY=0.145354
PRIMER_RIGHT_3_PENALTY=0.203343
PRIMER_LEFT_3_SEQUENCE=GAGGATGAGGGTAGGTATGCAC
PRIMER_RIGHT_3_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=59.855
PRIMER_RIGHT_3_TM=60.203
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.3487
PRIMER_LEFT_4_PENALTY=0.145354
PRIMER_RIGHT_4_PENALTY=0.203343
PRIMER_LEFT_4_SEQUENCE=GTGAGGATGAGGGTAGGTATGC
PRIMER_RIGHT_4_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=205,22
PRIMER_LEFT_4_TM=59.855
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=168
=
PRIMER_SEQUENCE_ID=SSR_02364 Ps_contig_mira-and-tgicl-ass_franssen_20368
SEQUENCE=TAGGTGTGNACTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGTAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGTGGAGGAGAGTGGTACACTGGGTGTGGGTGAGGAGCACTACACAGAGATTGTGGATGACTTGCGTGGAGGTGATCCATGCATTGGTTCAGGATCTCAGGTGGCAAGCCAGGAGACATACGGAACTTTGGGNCCATTGNGAGGA
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1902
PRIMER_LEFT_PENALTY=0.145354
PRIMER_RIGHT_PENALTY=0.044832
PRIMER_LEFT_SEQUENCE=GAGGATGAGGGTAGGTATGCAC
PRIMER_RIGHT_SEQUENCE=ACACCCAGTGTACCACTCTCCT
PRIMER_LEFT=40,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=59.855
PRIMER_RIGHT_TM=59.955
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.3487
PRIMER_LEFT_1_PENALTY=0.145354
PRIMER_RIGHT_1_PENALTY=0.203343
PRIMER_LEFT_1_SEQUENCE=GAGGATGAGGGTAGGTATGCAC
PRIMER_RIGHT_1_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_LEFT_1=40,22
PRIMER_RIGHT_1=202,22
PRIMER_LEFT_1_TM=59.855
PRIMER_RIGHT_1_TM=60.203
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.3487
PRIMER_LEFT_2_PENALTY=0.145354
PRIMER_RIGHT_2_PENALTY=0.203343
PRIMER_LEFT_2_SEQUENCE=GTGAGGATGAGGGTAGGTATGC
PRIMER_RIGHT_2_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=202,22
PRIMER_LEFT_2_TM=59.855
PRIMER_RIGHT_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.3487
PRIMER_LEFT_3_PENALTY=0.145354
PRIMER_RIGHT_3_PENALTY=0.203343
PRIMER_LEFT_3_SEQUENCE=GAGGATGAGGGTAGGTATGCAC
PRIMER_RIGHT_3_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=59.855
PRIMER_RIGHT_3_TM=60.203
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.3487
PRIMER_LEFT_4_PENALTY=0.145354
PRIMER_RIGHT_4_PENALTY=0.203343
PRIMER_LEFT_4_SEQUENCE=GTGAGGATGAGGGTAGGTATGC
PRIMER_RIGHT_4_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=205,22
PRIMER_LEFT_4_TM=59.855
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=168
=
PRIMER_SEQUENCE_ID=SSR_02365 Ps_contig_mira-and-tgicl-ass_franssen_20368
SEQUENCE=TAGGTGTGNACTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGTAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGTGGAGGAGAGTGGTACACTGGGTGTGGGTGAGGAGCACTACACAGAGATTGTGGATGACTTGCGTGGAGGTGATCCATGCATTGGTTCAGGATCTCAGGTGGCAAGCCAGGAGACATACGGAACTTTGGGNCCATTGNGAGGA
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1902
PRIMER_LEFT_PENALTY=0.145354
PRIMER_RIGHT_PENALTY=0.044832
PRIMER_LEFT_SEQUENCE=GAGGATGAGGGTAGGTATGCAC
PRIMER_RIGHT_SEQUENCE=ACACCCAGTGTACCACTCTCCT
PRIMER_LEFT=40,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=59.855
PRIMER_RIGHT_TM=59.955
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.3487
PRIMER_LEFT_1_PENALTY=0.145354
PRIMER_RIGHT_1_PENALTY=0.203343
PRIMER_LEFT_1_SEQUENCE=GAGGATGAGGGTAGGTATGCAC
PRIMER_RIGHT_1_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_LEFT_1=40,22
PRIMER_RIGHT_1=202,22
PRIMER_LEFT_1_TM=59.855
PRIMER_RIGHT_1_TM=60.203
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.3487
PRIMER_LEFT_2_PENALTY=0.145354
PRIMER_RIGHT_2_PENALTY=0.203343
PRIMER_LEFT_2_SEQUENCE=GTGAGGATGAGGGTAGGTATGC
PRIMER_RIGHT_2_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=202,22
PRIMER_LEFT_2_TM=59.855
PRIMER_RIGHT_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.3487
PRIMER_LEFT_3_PENALTY=0.145354
PRIMER_RIGHT_3_PENALTY=0.203343
PRIMER_LEFT_3_SEQUENCE=GAGGATGAGGGTAGGTATGCAC
PRIMER_RIGHT_3_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=59.855
PRIMER_RIGHT_3_TM=60.203
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.3487
PRIMER_LEFT_4_PENALTY=0.145354
PRIMER_RIGHT_4_PENALTY=0.203343
PRIMER_LEFT_4_SEQUENCE=GTGAGGATGAGGGTAGGTATGC
PRIMER_RIGHT_4_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=205,22
PRIMER_LEFT_4_TM=59.855
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=168
=
PRIMER_SEQUENCE_ID=SSR_02366 Ps_contig_mira-and-tgicl-ass_franssen_20369
SEQUENCE=GGGGAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTTCACACYYACCMYCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCAYACTATATAGGATACAAACNGAAAGTGTAGAAAGCAAAAAGGAAAACGCCCTAAATAACCG
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02367 Ps_contig_mira-and-tgicl-ass_franssen_20369
SEQUENCE=GGGGAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTTCACACYYACCMYCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCAYACTATATAGGATACAAACNGAAAGTGTAGAAAGCAAAAAGGAAAACGCCCTAAATAACCG
TARGET=235,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02368 Ps_contig_mira-and-tgicl-ass_franssen_20369
SEQUENCE=GGGGAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTTCACACYYACCMYCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCAYACTATATAGGATACAAACNGAAAGTGTAGAAAGCAAAAAGGAAAACGCCCTAAATAACCG
TARGET=286,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02369 Ps_contig_mira-and-tgicl-ass_franssen_20369
SEQUENCE=GGGGAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTTCACACYYACCMYCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCAYACTATATAGGATACAAACNGAAAGTGTAGAAAGCAAAAAGGAAAACGCCCTAAATAACCG
TARGET=332,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02370 Ps_contig_mira-and-tgicl-ass_franssen_20369
SEQUENCE=GGGGAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTTCACACYYACCMYCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCAYACTATATAGGATACAAACNGAAAGTGTAGAAAGCAAAAAGGAAAACGCCCTAAATAACCG
TARGET=392,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02371 Ps_contig_mira-and-tgicl-ass_franssen_20370
SEQUENCE=ACTCCACATAAAAAGCCATACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCGGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCAGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGTCAATCATTGGCATCACACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTGTTTTTGTTGTATTAGCCACAGCTGTAAGGCACTGCCTCAGTATGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCTGACCGACAAGCCAAAATAAATATATCCCCTATTTTGACTATTACCCTAAAAATT
TARGET=37,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0516
PRIMER_LEFT_PENALTY=0.624930
PRIMER_RIGHT_PENALTY=0.426682
PRIMER_LEFT_SEQUENCE=GCCATACAAGTACCCATCTCCT
PRIMER_RIGHT_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT=14,22
PRIMER_RIGHT=177,22
PRIMER_LEFT_TM=59.375
PRIMER_RIGHT_TM=60.427
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=1.0516
PRIMER_LEFT_1_PENALTY=0.624930
PRIMER_RIGHT_1_PENALTY=0.426682
PRIMER_LEFT_1_SEQUENCE=AGCCATACAAGTACCCATCTCC
PRIMER_RIGHT_1_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT_1=13,22
PRIMER_RIGHT_1=177,22
PRIMER_LEFT_1_TM=59.375
PRIMER_RIGHT_1_TM=60.427
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=1.1387
PRIMER_LEFT_2_PENALTY=0.624930
PRIMER_RIGHT_2_PENALTY=0.513776
PRIMER_LEFT_2_SEQUENCE=GCCATACAAGTACCCATCTCCT
PRIMER_RIGHT_2_SEQUENCE=GAGTGGTAGACAGGGTGAGGAT
PRIMER_LEFT_2=14,22
PRIMER_RIGHT_2=178,22
PRIMER_LEFT_2_TM=59.375
PRIMER_RIGHT_2_TM=59.486
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=1.1387
PRIMER_LEFT_3_PENALTY=0.624930
PRIMER_RIGHT_3_PENALTY=0.513776
PRIMER_LEFT_3_SEQUENCE=AGCCATACAAGTACCCATCTCC
PRIMER_RIGHT_3_SEQUENCE=GAGTGGTAGACAGGGTGAGGAT
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=178,22
PRIMER_LEFT_3_TM=59.375
PRIMER_RIGHT_3_TM=59.486
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=1.6944
PRIMER_LEFT_4_PENALTY=1.267765
PRIMER_RIGHT_4_PENALTY=0.426682
PRIMER_LEFT_4_SEQUENCE=AGCCATACAAGTACCCATCTCCT
PRIMER_RIGHT_4_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT_4=13,23
PRIMER_RIGHT_4=177,22
PRIMER_LEFT_4_TM=60.268
PRIMER_RIGHT_4_TM=60.427
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_02372 Ps_contig_mira-and-tgicl-ass_franssen_20370
SEQUENCE=ACTCCACATAAAAAGCCATACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCGGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCAGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGTCAATCATTGGCATCACACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTGTTTTTGTTGTATTAGCCACAGCTGTAAGGCACTGCCTCAGTATGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCTGACCGACAAGCCAAAATAAATATATCCCCTATTTTGACTATTACCCTAAAAATT
TARGET=88,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5334
PRIMER_LEFT_PENALTY=0.246726
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT=63,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=60.247
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.7210
PRIMER_LEFT_1_PENALTY=0.246726
PRIMER_RIGHT_1_PENALTY=0.474295
PRIMER_LEFT_1_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_1_SEQUENCE=TGTATGGCTTCTTGTGTGGTGT
PRIMER_LEFT_1=63,22
PRIMER_RIGHT_1=267,22
PRIMER_LEFT_1_TM=60.247
PRIMER_RIGHT_1_TM=60.474
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.9596
PRIMER_LEFT_2_PENALTY=0.672968
PRIMER_RIGHT_2_PENALTY=0.286660
PRIMER_LEFT_2_SEQUENCE=ATCCTCACCCTGTCTACCATTC
PRIMER_RIGHT_2_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT_2=64,22
PRIMER_RIGHT_2=281,22
PRIMER_LEFT_2_TM=59.327
PRIMER_RIGHT_2_TM=59.713
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=1.0433
PRIMER_LEFT_3_PENALTY=0.246726
PRIMER_RIGHT_3_PENALTY=0.796583
PRIMER_LEFT_3_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_3_SEQUENCE=GAACATGAGGAGGGTAGGTGTG
PRIMER_LEFT_3=63,22
PRIMER_RIGHT_3=318,22
PRIMER_LEFT_3_TM=60.247
PRIMER_RIGHT_3_TM=60.797
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=1.0433
PRIMER_LEFT_4_PENALTY=0.246726
PRIMER_RIGHT_4_PENALTY=0.796583
PRIMER_LEFT_4_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_4_SEQUENCE=AACATGAGGAGGGTAGGTGTGA
PRIMER_LEFT_4=63,22
PRIMER_RIGHT_4=317,22
PRIMER_LEFT_4_TM=60.247
PRIMER_RIGHT_4_TM=60.797
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_02373 Ps_contig_mira-and-tgicl-ass_franssen_20370
SEQUENCE=ACTCCACATAAAAAGCCATACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCGGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCAGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGTCAATCATTGGCATCACACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTGTTTTTGTTGTATTAGCCACAGCTGTAAGGCACTGCCTCAGTATGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCTGACCGACAAGCCAAAATAAATATATCCCCTATTTTGACTATTACCCTAAAAATT
TARGET=130,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5334
PRIMER_LEFT_PENALTY=0.246726
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT=63,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=60.247
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.7210
PRIMER_LEFT_1_PENALTY=0.246726
PRIMER_RIGHT_1_PENALTY=0.474295
PRIMER_LEFT_1_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_1_SEQUENCE=TGTATGGCTTCTTGTGTGGTGT
PRIMER_LEFT_1=63,22
PRIMER_RIGHT_1=267,22
PRIMER_LEFT_1_TM=60.247
PRIMER_RIGHT_1_TM=60.474
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.9596
PRIMER_LEFT_2_PENALTY=0.672968
PRIMER_RIGHT_2_PENALTY=0.286660
PRIMER_LEFT_2_SEQUENCE=ATCCTCACCCTGTCTACCATTC
PRIMER_RIGHT_2_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT_2=64,22
PRIMER_RIGHT_2=281,22
PRIMER_LEFT_2_TM=59.327
PRIMER_RIGHT_2_TM=59.713
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=1.0433
PRIMER_LEFT_3_PENALTY=0.246726
PRIMER_RIGHT_3_PENALTY=0.796583
PRIMER_LEFT_3_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_3_SEQUENCE=GAACATGAGGAGGGTAGGTGTG
PRIMER_LEFT_3=63,22
PRIMER_RIGHT_3=318,22
PRIMER_LEFT_3_TM=60.247
PRIMER_RIGHT_3_TM=60.797
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=1.0433
PRIMER_LEFT_4_PENALTY=0.246726
PRIMER_RIGHT_4_PENALTY=0.796583
PRIMER_LEFT_4_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_4_SEQUENCE=AACATGAGGAGGGTAGGTGTGA
PRIMER_LEFT_4=63,22
PRIMER_RIGHT_4=317,22
PRIMER_LEFT_4_TM=60.247
PRIMER_RIGHT_4_TM=60.797
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_02374 Ps_contig_mira-and-tgicl-ass_franssen_20370
SEQUENCE=ACTCCACATAAAAAGCCATACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCGGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCAGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGTCAATCATTGGCATCACACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTGTTTTTGTTGTATTAGCCACAGCTGTAAGGCACTGCCTCAGTATGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCTGACCGACAAGCCAAAATAAATATATCCCCTATTTTGACTATTACCCTAAAAATT
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5334
PRIMER_LEFT_PENALTY=0.246726
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT=63,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=60.247
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.5627
PRIMER_LEFT_1_PENALTY=0.426682
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_1_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT_1=156,22
PRIMER_RIGHT_1=403,22
PRIMER_LEFT_1_TM=60.427
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.5627
PRIMER_LEFT_2_PENALTY=0.426682
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_2_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_2=156,22
PRIMER_RIGHT_2=404,22
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.5627
PRIMER_LEFT_3_PENALTY=0.426682
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_3_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_LEFT_3=156,22
PRIMER_RIGHT_3=402,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.6498
PRIMER_LEFT_4_PENALTY=0.513776
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=ATCCTCACCCTGTCTACCACTC
PRIMER_RIGHT_4_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_4=157,22
PRIMER_RIGHT_4=404,22
PRIMER_LEFT_4_TM=59.486
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_02375 Ps_contig_mira-and-tgicl-ass_franssen_20370
SEQUENCE=ACTCCACATAAAAAGCCATACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCGGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCAGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGTCAATCATTGGCATCACACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTGTTTTTGTTGTATTAGCCACAGCTGTAAGGCACTGCCTCAGTATGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCTGACCGACAAGCCAAAATAAATATATCCCCTATTTTGACTATTACCCTAAAAATT
TARGET=232,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5334
PRIMER_LEFT_PENALTY=0.246726
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT=63,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=60.247
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.5627
PRIMER_LEFT_1_PENALTY=0.426682
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_1_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT_1=156,22
PRIMER_RIGHT_1=403,22
PRIMER_LEFT_1_TM=60.427
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.5627
PRIMER_LEFT_2_PENALTY=0.426682
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_2_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_2=156,22
PRIMER_RIGHT_2=404,22
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.5627
PRIMER_LEFT_3_PENALTY=0.426682
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_3_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_LEFT_3=156,22
PRIMER_RIGHT_3=402,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.6498
PRIMER_LEFT_4_PENALTY=0.513776
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=ATCCTCACCCTGTCTACCACTC
PRIMER_RIGHT_4_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_4=157,22
PRIMER_RIGHT_4=404,22
PRIMER_LEFT_4_TM=59.486
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_02376 Ps_contig_mira-and-tgicl-ass_franssen_20370
SEQUENCE=ACTCCACATAAAAAGCCATACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCGGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCAGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGTCAATCATTGGCATCACACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTGTTTTTGTTGTATTAGCCACAGCTGTAAGGCACTGCCTCAGTATGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCTGACCGACAAGCCAAAATAAATATATCCCCTATTTTGACTATTACCCTAAAAATT
TARGET=278,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5407
PRIMER_LEFT_PENALTY=0.474295
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT=246,22
PRIMER_RIGHT=476,22
PRIMER_LEFT_TM=60.474
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.5627
PRIMER_LEFT_1_PENALTY=0.426682
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_1_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT_1=156,22
PRIMER_RIGHT_1=403,22
PRIMER_LEFT_1_TM=60.427
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.5627
PRIMER_LEFT_2_PENALTY=0.426682
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_2_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_2=156,22
PRIMER_RIGHT_2=404,22
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.5627
PRIMER_LEFT_3_PENALTY=0.426682
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_3_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_LEFT_3=156,22
PRIMER_RIGHT_3=402,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.6103
PRIMER_LEFT_4_PENALTY=0.474295
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_4_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_4=246,22
PRIMER_RIGHT_4=404,22
PRIMER_LEFT_4_TM=60.474
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_02377 Ps_contig_mira-and-tgicl-ass_franssen_20370
SEQUENCE=ACTCCACATAAAAAGCCATACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCGGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCAGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGTCAATCATTGGCATCACACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTGTTTTTGTTGTATTAGCCACAGCTGTAAGGCACTGCCTCAGTATGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCTGACCGACAAGCCAAAATAAATATATCCCCTATTTTGACTATTACCCTAAAAATT
TARGET=338,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3531
PRIMER_LEFT_PENALTY=0.286660
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=GCCATACAAGTATCCATCACCA
PRIMER_RIGHT_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT=260,22
PRIMER_RIGHT=476,22
PRIMER_LEFT_TM=59.713
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.4350
PRIMER_LEFT_1_PENALTY=0.368624
PRIMER_RIGHT_1_PENALTY=0.066394
PRIMER_LEFT_1_SEQUENCE=ATGTTCCTACCCCAGTTTACCA
PRIMER_RIGHT_1_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT_1=313,22
PRIMER_RIGHT_1=476,22
PRIMER_LEFT_1_TM=59.631
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.4350
PRIMER_LEFT_2_PENALTY=0.368624
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=TGTTCCTACCCCAGTTTACCAT
PRIMER_RIGHT_2_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT_2=314,22
PRIMER_RIGHT_2=476,22
PRIMER_LEFT_2_TM=59.631
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=0.4350
PRIMER_LEFT_3_PENALTY=0.368624
PRIMER_RIGHT_3_PENALTY=0.066394
PRIMER_LEFT_3_SEQUENCE=CATGTTCCTACCCCAGTTTACC
PRIMER_RIGHT_3_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT_3=312,22
PRIMER_RIGHT_3=476,22
PRIMER_LEFT_3_TM=59.631
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.4371
PRIMER_LEFT_4_PENALTY=0.368624
PRIMER_RIGHT_4_PENALTY=0.068468
PRIMER_LEFT_4_SEQUENCE=ATGTTCCTACCCCAGTTTACCA
PRIMER_RIGHT_4_SEQUENCE=TGCCTTACAGCTGTGGCTAATA
PRIMER_LEFT_4=313,22
PRIMER_RIGHT_4=602,22
PRIMER_LEFT_4_TM=59.631
PRIMER_RIGHT_4_TM=59.932
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=5.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_02378 Ps_contig_mira-and-tgicl-ass_franssen_20370
SEQUENCE=ACTCCACATAAAAAGCCATACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCGGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCAGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGTCAATCATTGGCATCACACTATATGGATCTAAACAGAAAGTGTGAAAGCAAAGAAAGCCATACACAAAGAAGATGAGAGGATTCTTCACATATATATTTCGTCAGTCTTTGATTAATATTGTTTAGAGGGTTGCTTTTATTTATTTAATTTGTGTTTTTGTTGTATTAGCCACAGCTGTAAGGCACTGCCTCAGTATGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCTGACCGACAAGCCAAAATAAATATATCCCCTATTTTGACTATTACCCTAAAAATT
TARGET=554,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1349
PRIMER_LEFT_PENALTY=0.066394
PRIMER_RIGHT_PENALTY=0.068468
PRIMER_LEFT_SEQUENCE=AAGTGTGAAAGCAAAGAAAGCC
PRIMER_RIGHT_SEQUENCE=TGCCTTACAGCTGTGGCTAATA
PRIMER_LEFT=455,22
PRIMER_RIGHT=602,22
PRIMER_LEFT_TM=59.934
PRIMER_RIGHT_TM=59.932
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=5.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=0.2045
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.068468
PRIMER_LEFT_1_SEQUENCE=CAAATCACCTCCTCCACCTTAC
PRIMER_RIGHT_1_SEQUENCE=TGCCTTACAGCTGTGGCTAATA
PRIMER_LEFT_1=383,22
PRIMER_RIGHT_1=602,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=59.932
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=5.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=220
PRIMER_PAIR_PENALTY_2=0.2045
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.068468
PRIMER_LEFT_2_SEQUENCE=TACAAATCACCTCCTCCACCTT
PRIMER_RIGHT_2_SEQUENCE=TGCCTTACAGCTGTGGCTAATA
PRIMER_LEFT_2=381,22
PRIMER_RIGHT_2=602,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.932
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.2045
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.068468
PRIMER_LEFT_3_SEQUENCE=ACAAATCACCTCCTCCACCTTA
PRIMER_RIGHT_3_SEQUENCE=TGCCTTACAGCTGTGGCTAATA
PRIMER_LEFT_3=382,22
PRIMER_RIGHT_3=602,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.932
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=5.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.2280
PRIMER_LEFT_4_PENALTY=0.066394
PRIMER_RIGHT_4_PENALTY=0.161579
PRIMER_LEFT_4_SEQUENCE=AAGTGTGAAAGCAAAGAAAGCC
PRIMER_RIGHT_4_SEQUENCE=ATTCATGCATACTGAGGCAGTG
PRIMER_LEFT_4=455,22
PRIMER_RIGHT_4=622,22
PRIMER_LEFT_4_TM=59.934
PRIMER_RIGHT_4_TM=60.162
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=168
=
PRIMER_SEQUENCE_ID=SSR_02379 Ps_contig_mira-and-tgicl-ass_franssen_20371
SEQUENCE=GTCGGGGTATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACYCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGCTCACACCTACCCTAAG
TARGET=138,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02380 Ps_contig_mira-and-tgicl-ass_franssen_20371
SEQUENCE=GTCGGGGTATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACYCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGCTCACACCTACCCTAAG
TARGET=251,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02381 Ps_contig_mira-and-tgicl-ass_franssen_20371
SEQUENCE=GTCGGGGTATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACYCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGCTCACACCTACCCTAAG
TARGET=294,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02382 Ps_contig_mira-and-tgicl-ass_franssen_20371
SEQUENCE=GTCGGGGTATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACYCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGCTCACACCTACCCTAAG
TARGET=350,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02383 Ps_contig_mira-and-tgicl-ass_franssen_20371
SEQUENCE=GTCGGGGTATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACYCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGCTCACACCTACCCTAAG
TARGET=392,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02384 Ps_contig_mira-and-tgicl-ass_franssen_20371
SEQUENCE=GTCGGGGTATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACYCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGCTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGCTCACACCTACCCTAAG
TARGET=443,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02385 Ps_contig_mira-and-tgicl-ass_franssen_20372
SEQUENCE=GTCGGGGAACCCAAAATAACACTTGTTAAGAAAACAATGAGGTCCCTAATGGCCTCTGCGTGCTCTCATTTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCACACTATAAG
TARGET=139,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0425
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.016950
PRIMER_LEFT_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_SEQUENCE=GTAATGGTGTGGATCCTTTGGT
PRIMER_LEFT=79,22
PRIMER_RIGHT=190,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=59.983
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=112
PRIMER_PAIR_PENALTY_1=0.0617
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.036173
PRIMER_LEFT_1_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_1_SEQUENCE=GTGGTGGAGGAGAGTGGTAGAC
PRIMER_LEFT_1=79,22
PRIMER_RIGHT_1=311,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=60.036
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=59.091
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.1351
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_2_SEQUENCE=AGTAATGGTGTGGATCCTTTGG
PRIMER_LEFT_2=79,22
PRIMER_RIGHT_2=191,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=0.1430
PRIMER_LEFT_3_PENALTY=0.025566
PRIMER_RIGHT_3_PENALTY=0.117452
PRIMER_LEFT_3_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_3_SEQUENCE=GAATGGTAGACTGGGTGTGGAT
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=60.117
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.1953
PRIMER_LEFT_4_PENALTY=0.025566
PRIMER_RIGHT_4_PENALTY=0.169745
PRIMER_LEFT_4_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_4_SEQUENCE=GAGGAGAGTGGTAGACTGGGTG
PRIMER_LEFT_4=79,22
PRIMER_RIGHT_4=305,22
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=60.170
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=59.091
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_02386 Ps_contig_mira-and-tgicl-ass_franssen_20372
SEQUENCE=GTCGGGGAACCCAAAATAACACTTGTTAAGAAAACAATGAGGTCCCTAATGGCCTCTGCGTGCTCTCATTTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCACACTATAAG
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0617
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.036173
PRIMER_LEFT_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_SEQUENCE=GTGGTGGAGGAGAGTGGTAGAC
PRIMER_LEFT=79,22
PRIMER_RIGHT=311,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=60.036
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=59.091
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.1430
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.117452
PRIMER_LEFT_1_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_1_SEQUENCE=GAATGGTAGACTGGGTGTGGAT
PRIMER_LEFT_1=79,22
PRIMER_RIGHT_1=249,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=60.117
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=171
PRIMER_PAIR_PENALTY_2=0.1953
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=0.169745
PRIMER_LEFT_2_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_2_SEQUENCE=GAGGAGAGTGGTAGACTGGGTG
PRIMER_LEFT_2=79,22
PRIMER_RIGHT_2=305,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=60.170
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=59.091
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=0.1953
PRIMER_LEFT_3_PENALTY=0.025566
PRIMER_RIGHT_3_PENALTY=0.169745
PRIMER_LEFT_3_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_3_SEQUENCE=GTGGAGGAGAGTGGTAGACTGG
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=308,22
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=60.170
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=59.091
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=0.1953
PRIMER_LEFT_4_PENALTY=0.025566
PRIMER_RIGHT_4_PENALTY=0.169745
PRIMER_LEFT_4_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_4_SEQUENCE=GGTGGAGGAGAGTGGTAGACTG
PRIMER_LEFT_4=79,22
PRIMER_RIGHT_4=309,22
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=60.170
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=59.091
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_02387 Ps_contig_mira-and-tgicl-ass_franssen_20372
SEQUENCE=GTCGGGGAACCCAAAATAACACTTGTTAAGAAAACAATGAGGTCCCTAATGGCCTCTGCGTGCTCTCATTTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCACACTATAAG
TARGET=351,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3959
PRIMER_LEFT_PENALTY=0.109200
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=TCACACTTACCCTCATCCACAC
PRIMER_RIGHT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT=214,22
PRIMER_RIGHT=451,22
PRIMER_LEFT_TM=59.891
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.4629
PRIMER_LEFT_1_PENALTY=0.036173
PRIMER_RIGHT_1_PENALTY=0.426682
PRIMER_LEFT_1_SEQUENCE=GTCTACCACTCTCCTCCACCAC
PRIMER_RIGHT_1_SEQUENCE=AGTGATAGACAGGGTGAGGGTG
PRIMER_LEFT_1=290,22
PRIMER_RIGHT_1=398,22
PRIMER_LEFT_1_TM=60.036
PRIMER_RIGHT_1_TM=60.427
PRIMER_LEFT_1_GC_PERCENT=59.091
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.5917
PRIMER_LEFT_2_PENALTY=0.117452
PRIMER_RIGHT_2_PENALTY=0.474295
PRIMER_LEFT_2_SEQUENCE=ATCCACACCCAGTCTACCATTC
PRIMER_RIGHT_2_SEQUENCE=TGTATGGCTTCTTGTGTGGTGT
PRIMER_LEFT_2=228,22
PRIMER_RIGHT_2=437,22
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=60.474
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.5964
PRIMER_LEFT_3_PENALTY=0.169745
PRIMER_RIGHT_3_PENALTY=0.426682
PRIMER_LEFT_3_SEQUENCE=AGTCTACCACTCTCCTCCACCA
PRIMER_RIGHT_3_SEQUENCE=AGTGATAGACAGGGTGAGGGTG
PRIMER_LEFT_3=289,22
PRIMER_RIGHT_3=398,22
PRIMER_LEFT_3_TM=60.170
PRIMER_RIGHT_3_TM=60.427
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=110
PRIMER_PAIR_PENALTY_4=0.6440
PRIMER_LEFT_4_PENALTY=0.169745
PRIMER_RIGHT_4_PENALTY=0.474295
PRIMER_LEFT_4_SEQUENCE=CACCCAGTCTACCACTCTCCTC
PRIMER_RIGHT_4_SEQUENCE=TGTATGGCTTCTTGTGTGGTGT
PRIMER_LEFT_4=284,22
PRIMER_RIGHT_4=437,22
PRIMER_LEFT_4_TM=60.170
PRIMER_RIGHT_4_TM=60.474
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_02388 Ps_contig_mira-and-tgicl-ass_franssen_20372
SEQUENCE=GTCGGGGAACCCAAAATAACACTTGTTAAGAAAACAATGAGGTCCCTAATGGCCTCTGCGTGCTCTCATTTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCACACTATAAG
TARGET=402,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3959
PRIMER_LEFT_PENALTY=0.109200
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=TCACACTTACCCTCATCCACAC
PRIMER_RIGHT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT=214,22
PRIMER_RIGHT=451,22
PRIMER_LEFT_TM=59.891
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.5627
PRIMER_LEFT_1_PENALTY=0.426682
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CACCCTCACCCTGTCTATCACT
PRIMER_RIGHT_1_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT_1=377,22
PRIMER_RIGHT_1=573,22
PRIMER_LEFT_1_TM=60.427
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=0.5627
PRIMER_LEFT_2_PENALTY=0.426682
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CACCCTCACCCTGTCTATCACT
PRIMER_RIGHT_2_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_2=377,22
PRIMER_RIGHT_2=574,22
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=0.5627
PRIMER_LEFT_3_PENALTY=0.426682
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CACCCTCACCCTGTCTATCACT
PRIMER_RIGHT_3_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_LEFT_3=377,22
PRIMER_RIGHT_3=572,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=0.6498
PRIMER_LEFT_4_PENALTY=0.513776
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=ACCCTCACCCTGTCTATCACTC
PRIMER_RIGHT_4_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT_4=378,22
PRIMER_RIGHT_4=573,22
PRIMER_LEFT_4_TM=59.486
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=196
=
PRIMER_SEQUENCE_ID=SSR_02389 Ps_contig_mira-and-tgicl-ass_franssen_20372
SEQUENCE=GTCGGGGAACCCAAAATAACACTTGTTAAGAAAACAATGAGGTCCCTAATGGCCTCTGCGTGCTCTCATTTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCACACTATAAG
TARGET=448,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5627
PRIMER_LEFT_PENALTY=0.426682
PRIMER_RIGHT_PENALTY=0.136017
PRIMER_LEFT_SEQUENCE=CACCCTCACCCTGTCTATCACT
PRIMER_RIGHT_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT=377,22
PRIMER_RIGHT=573,22
PRIMER_LEFT_TM=60.427
PRIMER_RIGHT_TM=59.864
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=0.5627
PRIMER_LEFT_1_PENALTY=0.426682
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CACCCTCACCCTGTCTATCACT
PRIMER_RIGHT_1_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_1=377,22
PRIMER_RIGHT_1=574,22
PRIMER_LEFT_1_TM=60.427
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.5627
PRIMER_LEFT_2_PENALTY=0.426682
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CACCCTCACCCTGTCTATCACT
PRIMER_RIGHT_2_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_LEFT_2=377,22
PRIMER_RIGHT_2=572,22
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.6103
PRIMER_LEFT_3_PENALTY=0.474295
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_3_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_3=416,22
PRIMER_RIGHT_3=574,22
PRIMER_LEFT_3_TM=60.474
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=0.6103
PRIMER_LEFT_4_PENALTY=0.474295
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_4_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT_4=416,22
PRIMER_RIGHT_4=573,22
PRIMER_LEFT_4_TM=60.474
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_02390 Ps_contig_mira-and-tgicl-ass_franssen_20372
SEQUENCE=GTCGGGGAACCCAAAATAACACTTGTTAAGAAAACAATGAGGTCCCTAATGGCCTCTGCGTGCTCTCATTTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCACACCATTACTCATCTCCTCCACCTCCACCGGTTCACACTTACCCTCATCCACACCCAGTCTACCATTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCACCACCAACACCACATAAGAAGCCATACAAGTACCCATCTCCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTATCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCACCCCAGTTTACCATTCTCCTCCTCCTCCAGCTTATTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCACATCATTGGCATCACACTATAAG
TARGET=508,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5627
PRIMER_LEFT_PENALTY=0.426682
PRIMER_RIGHT_PENALTY=0.136017
PRIMER_LEFT_SEQUENCE=CACCCTCACCCTGTCTATCACT
PRIMER_RIGHT_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT=377,22
PRIMER_RIGHT=573,22
PRIMER_LEFT_TM=60.427
PRIMER_RIGHT_TM=59.864
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=0.5627
PRIMER_LEFT_1_PENALTY=0.426682
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CACCCTCACCCTGTCTATCACT
PRIMER_RIGHT_1_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_1=377,22
PRIMER_RIGHT_1=574,22
PRIMER_LEFT_1_TM=60.427
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.5627
PRIMER_LEFT_2_PENALTY=0.426682
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CACCCTCACCCTGTCTATCACT
PRIMER_RIGHT_2_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_LEFT_2=377,22
PRIMER_RIGHT_2=572,22
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.6103
PRIMER_LEFT_3_PENALTY=0.474295
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_3_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_3=416,22
PRIMER_RIGHT_3=574,22
PRIMER_LEFT_3_TM=60.474
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=0.6103
PRIMER_LEFT_4_PENALTY=0.474295
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_4_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT_4=416,22
PRIMER_RIGHT_4=573,22
PRIMER_LEFT_4_TM=60.474
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_02391 Ps_contig_mira-and-tgicl-ass_franssen_20381
SEQUENCE=AAGGACAGAGGTGTTTTTGATTTTCTCGGTGGTAAGTTTAAAAAGGTTTATTGAAGAACATAAGCCTGCTCAAGATGAGGCGATTGCCCACGAGTTTGGTCACAAGGTGACTTTGTATGAAGCACCGTCGGAAACCAAAGTTGAAGAGGAAGGTGAAAAGAAACACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTCAAGTGAAGAGGAAGGAGAAGATGGTGAAGAAAAGAAAGAAGAAGAAGGAAANAGAAGAAGAAGAACGAACKTACGGTACGGTACCGTACGGTTACGGTAAGGTAAAGGTTAGGTAAGGGTTAGGTTAGGTAAGGGTAAACGAAACCGGGTAACCGGAAACGGGTAAAAACGGG
TARGET=252,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02392 Ps_contig_mira-and-tgicl-ass_franssen_20381
SEQUENCE=AAGGACAGAGGTGTTTTTGATTTTCTCGGTGGTAAGTTTAAAAAGGTTTATTGAAGAACATAAGCCTGCTCAAGATGAGGCGATTGCCCACGAGTTTGGTCACAAGGTGACTTTGTATGAAGCACCGTCGGAAACCAAAGTTGAAGAGGAAGGTGAAAAGAAACACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTCAAGTGAAGAGGAAGGAGAAGATGGTGAAGAAAAGAAAGAAGAAGAAGGAAANAGAAGAAGAAGAACGAACKTACGGTACGGTACCGTACGGTTACGGTAAGGTAAAGGTTAGGTAAGGGTTAGGTTAGGTAAGGGTAAACGAAACCGGGTAACCGGAAACGGGTAAAAACGGG
TARGET=269,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02393 Ps_contig_mira-and-tgicl-ass_franssen_20387
SEQUENCE=TGTTTGGTAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATTACCGTTCTTACAAATGGACGAAAACAGAGWAAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAATATAATTGGTTGTAGATTACAGTATACTTCTTAGTGTGTTGTGAGTTCTTTTTTTACTGTGACACTTTCTA
TARGET=168,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02394 Ps_contig_mira-and-tgicl-ass_franssen_20390
SEQUENCE=GTCGGGGGATACACCAACATACATACATATCTTCTTCTCAAACTCCTTTTTATCAAATCAATAATCATGGGTGTTTTCACATATGAGAATGATACCACCTCTACCGTCCCTCCTGCCAAGCTCTTCAAAGCTGTCGTGCATGACGCTGATCTYATCCGTACCCAAAAGTTGTTGATTCAATCAAGACTGTTAGAAATCTGAAGGAATGGTGGTCCAGGCTACG
TARGET=17,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02395 Ps_contig_mira-and-tgicl-ass_franssen_20408
SEQUENCE=AAACAAAAGAATAAATAACCAAGTAATTCACATTATATATAGTTTGCCAATAATATCATACAGTAATATGAGACAAGTGGAACATTTCTTACACCAACATTCACCATTTCCAGCCATAATAATAATAATACACAAGTTTGACATGATTACAACAATCTATGACGATGATGAAGACACTCAAGAGGAGTAACAGGAAACCTAGAAGCATCATTACAATAATCATAAACCATATGTTTAGCCCTAACCCACATTAGTTGATAGACTCTGATGCAAATTAAGCTCTGAC
TARGET=116,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5159
PRIMER_LEFT_PENALTY=0.408180
PRIMER_RIGHT_PENALTY=0.107763
PRIMER_LEFT_SEQUENCE=TACACCAACATTCACCATTTCC
PRIMER_RIGHT_SEQUENCE=TCAACTAATGTGGGTTAGGGCT
PRIMER_LEFT=89,22
PRIMER_RIGHT=257,22
PRIMER_LEFT_TM=59.592
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=1.1405
PRIMER_LEFT_1_PENALTY=0.408180
PRIMER_RIGHT_1_PENALTY=0.732310
PRIMER_LEFT_1_SEQUENCE=TACACCAACATTCACCATTTCC
PRIMER_RIGHT_1_SEQUENCE=ATCAACTAATGTGGGTTAGGGC
PRIMER_LEFT_1=89,22
PRIMER_RIGHT_1=258,22
PRIMER_LEFT_1_TM=59.592
PRIMER_RIGHT_1_TM=59.268
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=1.4052
PRIMER_LEFT_2_PENALTY=1.297449
PRIMER_RIGHT_2_PENALTY=0.107763
PRIMER_LEFT_2_SEQUENCE=ACCAACATTCACCATTTCCAG
PRIMER_RIGHT_2_SEQUENCE=TCAACTAATGTGGGTTAGGGCT
PRIMER_LEFT_2=92,21
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=59.703
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=1.5430
PRIMER_LEFT_3_PENALTY=1.435246
PRIMER_RIGHT_3_PENALTY=0.107763
PRIMER_LEFT_3_SEQUENCE=ACACCAACATTCACCATTTCC
PRIMER_RIGHT_3_SEQUENCE=TCAACTAATGTGGGTTAGGGCT
PRIMER_LEFT_3=90,21
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=59.565
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=1.5486
PRIMER_LEFT_4_PENALTY=0.408180
PRIMER_RIGHT_4_PENALTY=1.140398
PRIMER_LEFT_4_SEQUENCE=TACACCAACATTCACCATTTCC
PRIMER_RIGHT_4_SEQUENCE=ATCAACTAATGTGGGTTAGGGCT
PRIMER_LEFT_4=89,22
PRIMER_RIGHT_4=258,23
PRIMER_LEFT_4_TM=59.592
PRIMER_RIGHT_4_TM=60.140
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_02396 Ps_contig_mira-and-tgicl-ass_franssen_20413
SEQUENCE=TTAGATAATTAATTCTTTAATTAATTAAGCATCACATGGCACTCTCATATACTAGTGAGAAGTTTCCATAAGATAAATCTTTTAAAACCGTACGCCATCATCTTCAAAACTTAAATGGTGTTACAGTTGGTGGAGGTACCGATCTTGTAAGGAATGCTAACACCGCATTTACCAGGGAGAGCAGCAGCATTGTTAGTATTCAAATTTAGAAATAGAACCGGCAGCTGATTTCAAACAGTTACAGGCAGCCTGACGATCAGGCGTGGTGGTGGCGGCAGCAAGAGCTTCTTCACTCCTGCACAGCATGGCGGTGGAGGCTGGCATTTATTAGGNGCTTAGAAGATAAGTAGGCAGTGGGACGACCAAATCCGACCGGATACAGTTCCA
TARGET=17,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02397 Ps_contig_mira-and-tgicl-ass_franssen_20417
SEQUENCE=TGTTTGGTAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTCACTCACCGCTTGTGCATTTAGAAAATTGAGGGATGCGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGTAGTTTCTTTTTTTACTGTGTACTA
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02398 Ps_contig_mira-and-tgicl-ass_franssen_20433
SEQUENCE=ACAGGGTGAGGATGAGGGTAGGTGTGCTACTTGGTTGGTGGTGGAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGTGGAGGAGAGTGGTGCACTGGGTGTGGGTGAGGGTAGGTGTGAACCGGTGGTGGTGGAGAGTGGTAGACTGGGTGTGGGTGAGGATAGGTGTGAGCTGGTGGAGGTGGAGGAGATGAGTAATGGTATGGATTCCTTTGGGTGGCGGTGGTGAGTGGACCGGTGGTGGTGGTGAGGAATAAG
TARGET=246,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02399 Ps_contig_mira-and-tgicl-ass_franssen_20441
SEQUENCE=CTACCCTCCATCTTGTGTTGCGACTTCGTGGTGGTATGCAGATTTTTGTGAAGACCCTTACTGGCAAGACAATTACTTTGGAGGTAGAGAGCTCTGACACAATTGACAATGTGAAGGCTAAGATTCAAGACAAGGAGGGTATTCCACCAGACCAGCAAAGGCGTCATCTTTGCCGGAAAGCAGGCTTGGAAGATGGCCGAACTTTGGCCGATTACAACATCCAGAAAGAATCAACCCTCCATCTTGTTTTGAGACTTCGTGGTGGTATGCAGATTTTTGTGAAGACCCTCACCGGCAAGACAATTACTTTGGAGGTGAGAGCTCAGACACAATTGAACAATGTGAAGGCCAAGATACAGGACAAGGAGGGTATCCCCACGACGACGACGAAACGAAGTTTGTAWTTTTTGCTAGGAAAACACTACGGAACGTAGTAAGGAACGTAACCGTCGGACTTGGACTACTAGAACAC
TARGET=378,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02400 Ps_contig_mira-and-tgicl-ass_franssen_20451
SEQUENCE=ACACAAAAAAAASACACCAAGTCCTAGTTGTAAGTCTTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAARCTTGCTTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGACCCTTTAAACCAAAAGAAGTAGTTTTCTAGCTTGTTACCAAAACCATAACGTACCCAAAAACAAACTACCCCATCTACAAAGAACAAACTRSTTCCATATATTCGG
TARGET=64,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02401 Ps_contig_mira-and-tgicl-ass_franssen_20454
SEQUENCE=TGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTAGGTGATAGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGGTGGCGGGGAATGGTAGACTGGGTGAGGATGGTAGGGTAGGTTATGGTCACGTGGTGGGTAGGTGGGGGAAGAGTTGGAGGTACTTAGTAGTGGTTTAC
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02402 Ps_contig_mira-and-tgicl-ass_franssen_20454
SEQUENCE=TGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTAGGTGATAGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGGTGGCGGGGAATGGTAGACTGGGTGAGGATGGTAGGGTAGGTTATGGTCACGTGGTGGGTAGGTGGGGGAAGAGTTGGAGGTACTTAGTAGTGGTTTAC
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9332
PRIMER_LEFT_PENALTY=0.586117
PRIMER_RIGHT_PENALTY=0.347129
PRIMER_LEFT_SEQUENCE=GAGGAGAATGGTAAACTGGGGT
PRIMER_RIGHT_SEQUENCE=CGTGACCATAACCTACCCTACC
PRIMER_LEFT=20,22
PRIMER_RIGHT=178,22
PRIMER_LEFT_TM=60.586
PRIMER_RIGHT_TM=59.653
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=159
PRIMER_PAIR_PENALTY_1=1.0433
PRIMER_LEFT_1_PENALTY=0.796583
PRIMER_RIGHT_1_PENALTY=0.246726
PRIMER_LEFT_1_SEQUENCE=AACATGAGGAGGGTAGGTGTGA
PRIMER_RIGHT_1_SEQUENCE=CATCCTCACCCAGTCTACCATT
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=157,22
PRIMER_LEFT_1_TM=60.797
PRIMER_RIGHT_1_TM=60.247
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=1.0514
PRIMER_LEFT_2_PENALTY=0.704252
PRIMER_RIGHT_2_PENALTY=0.347129
PRIMER_LEFT_2_SEQUENCE=AGGAGGAGAATGGTAAACTGGG
PRIMER_RIGHT_2_SEQUENCE=CGTGACCATAACCTACCCTACC
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=178,22
PRIMER_LEFT_2_TM=60.704
PRIMER_RIGHT_2_TM=59.653
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=1.1304
PRIMER_LEFT_3_PENALTY=0.783306
PRIMER_RIGHT_3_PENALTY=0.347129
PRIMER_LEFT_3_SEQUENCE=GGAATAAACTGGAGGAGGAGGA
PRIMER_RIGHT_3_SEQUENCE=CGTGACCATAACCTACCCTACC
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=178,22
PRIMER_LEFT_3_TM=60.783
PRIMER_RIGHT_3_TM=59.653
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=1.1573
PRIMER_LEFT_4_PENALTY=0.783306
PRIMER_RIGHT_4_PENALTY=0.373998
PRIMER_LEFT_4_SEQUENCE=GGAGGAGGAGGAGAATGGTAAA
PRIMER_RIGHT_4_SEQUENCE=CCTACCATCCTCACCCAGTCTA
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=162,22
PRIMER_LEFT_4_TM=60.783
PRIMER_RIGHT_4_TM=60.374
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_02403 Ps_contig_mira-and-tgicl-ass_franssen_20468
SEQUENCE=AGTTTCAAACAAACAAAGACRTAGATAAGTATTATGAATCATAATCATAACATTACATGACAAAGGGTAGACAWAAATGTCTAAATTAATGCAATTTAAACATCCCTCGGCAACAAAATGTACTACTCAAACATAAATTAACACCATAAAAACAGTAACAAGGAAAATCAACAAAATAGGAAAATTAGACCACCGCCGATCAAGAACCCTAGTTTTGTTAAGTTTAGTTTAATGAAACAGGACAAAAACTTACAAAATTAAACTGGAAAGATAAACTACTATAAAGGAACAACTTGTGCAGAAGCAACCATGGAGTGAAATCACTTCTTCTTGGCTGCTGCTTTGGTGACCTTGGCTCCAGTTGGGTCCTTCTTCTCCACACTCTTGATGACTCCGACAGCAACAGTTTGTCTCATGTCCCTCACAGCAAAACGACCAAGAGGAGGATACTCAGCAAAAGTTTCCACCACCATGGGCTTGGTTGGAATCATC
TARGET=6,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02404 Ps_contig_mira-and-tgicl-ass_franssen_20472
SEQUENCE=TACACACATAACTTAATTCAAATACATACACAGATACANCGGAGTTGCTATAGCTGCATGCATTTATTTATTTATATTATTATATTTATAACGTCTTTAACAGTTTTGAGTGCNGAAACATTTCCAGTCATGGCACGTGCCACTGATTAAGTGCGCTTTGTTCTTGCAGTGATCATCACAGCTAGCATTCGTGAAGCATACTCCCCTGTATGTATCGGCCAAATGCTCACAAGTTTTTGCCTCTGTCACCACAATTTCTTGTGCAACAAAGAGAACGAGGAAGAGGAAGGACAAGGCAGCTACTGCTTTCTTCTCCATGACTTAAGTGTGGTATGAATATAATAAACTTAATATAACTCACTTCAAA
TARGET=62,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9495
PRIMER_LEFT_PENALTY=0.940537
PRIMER_RIGHT_PENALTY=0.008930
PRIMER_LEFT_SEQUENCE=CACAGATACANCGGAGTTGCTA
PRIMER_RIGHT_SEQUENCE=TTCCTCTTCCTCGTTCTCTTTG
PRIMER_LEFT=28,22
PRIMER_RIGHT=287,22
PRIMER_LEFT_TM=59.059
PRIMER_RIGHT_TM=59.991
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.50
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.50
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=1.0891
PRIMER_LEFT_1_PENALTY=1.080186
PRIMER_RIGHT_1_PENALTY=0.008930
PRIMER_LEFT_1_SEQUENCE=TACACAGATACANCGGAGTTGC
PRIMER_RIGHT_1_SEQUENCE=TTCCTCTTCCTCGTTCTCTTTG
PRIMER_LEFT_1=26,22
PRIMER_RIGHT_1=287,22
PRIMER_LEFT_1_TM=58.920
PRIMER_RIGHT_1_TM=59.991
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.50
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=1.1296
PRIMER_LEFT_2_PENALTY=1.120688
PRIMER_RIGHT_2_PENALTY=0.008930
PRIMER_LEFT_2_SEQUENCE=ACACAGATACANCGGAGTTGCTA
PRIMER_RIGHT_2_SEQUENCE=TTCCTCTTCCTCGTTCTCTTTG
PRIMER_LEFT_2=27,23
PRIMER_RIGHT_2=287,22
PRIMER_LEFT_2_TM=59.879
PRIMER_RIGHT_2_TM=59.991
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.50
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.50
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=1.2403
PRIMER_LEFT_3_PENALTY=0.940537
PRIMER_RIGHT_3_PENALTY=0.299723
PRIMER_LEFT_3_SEQUENCE=CACAGATACANCGGAGTTGCTA
PRIMER_RIGHT_3_SEQUENCE=CACGAATGCTAGCTGTGATGAT
PRIMER_LEFT_3=28,22
PRIMER_RIGHT_3=192,22
PRIMER_LEFT_3_TM=59.059
PRIMER_RIGHT_3_TM=60.300
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.50
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.50
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=1.2432
PRIMER_LEFT_4_PENALTY=0.940537
PRIMER_RIGHT_4_PENALTY=0.302618
PRIMER_LEFT_4_SEQUENCE=CACAGATACANCGGAGTTGCTA
PRIMER_RIGHT_4_SEQUENCE=AGAGGCAAAAACTTGTGAGCAT
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=243,22
PRIMER_LEFT_4_TM=59.059
PRIMER_RIGHT_4_TM=60.303
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.50
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.50
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_02405 Ps_contig_mira-and-tgicl-ass_franssen_20473
SEQUENCE=TGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGGGGATAGGTGTGCACTGGTGGTGTATTCGAATGCATGTGCTACCTTCAATGTCCCTCTTGACGTAACCACAAATATCAAAGGAGCTTTAGCCCATCCCCAGGAGTTTATGCATCACGTAGGTCTGTATATGGCCAAAGAACGTGGAGCCAAGATAGCAAAGGAGTGTTGTTTGGTCAGTAGAGAAAGTATAACATAGCACTTATCAAATAAAACTGTAAGATATATTTTAAGGTTGGTTGGTCTAAATAGTAGCAAATGGTCCTAGGTGACATATCATCAAATGTAAAATNTATCGCCCCTTCCCTTGTTTTAGGGTAAAGG
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4445
PRIMER_LEFT_PENALTY=0.426682
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_RIGHT_SEQUENCE=TACGTGATGCATAAACTCCTGG
PRIMER_LEFT=8,22
PRIMER_RIGHT=238,22
PRIMER_LEFT_TM=60.427
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.5222
PRIMER_LEFT_1_PENALTY=0.426682
PRIMER_RIGHT_1_PENALTY=0.095538
PRIMER_LEFT_1_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_RIGHT_1_SEQUENCE=TGGCCATATACAGACCTACGTG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=254,22
PRIMER_LEFT_1_TM=60.427
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.5316
PRIMER_LEFT_2_PENALTY=0.513776
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=GAGTGGTAGACAGGGTGAGGAT
PRIMER_RIGHT_2_SEQUENCE=TACGTGATGCATAAACTCCTGG
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=238,22
PRIMER_LEFT_2_TM=59.486
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.5349
PRIMER_LEFT_3_PENALTY=0.426682
PRIMER_RIGHT_3_PENALTY=0.108206
PRIMER_LEFT_3_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_RIGHT_3_SEQUENCE=AGGGACATTGAAGGTAGCACAT
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=175,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=0.5570
PRIMER_LEFT_4_PENALTY=0.426682
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_RIGHT_4_SEQUENCE=AAGAGGGACATTGAAGGTAGCA
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=60.427
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_02406 Ps_contig_mira-and-tgicl-ass_franssen_20485
SEQUENCE=CGTATCCCTTGTTCCGAAAACTTGGCATCTCCATATAGTGTGATGCCAATGATTGACTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTAAACCGGAGGAGGAGGAGAATGGTAAACTGGGGTAGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGCGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCCAATGCTAATATTGATTATTGGATTTGATGACAAAATCAGCTA
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2766
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.140609
PRIMER_LEFT_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_RIGHT_SEQUENCE=TCAATATTAGCATTGGCACACC
PRIMER_LEFT=70,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=59.859
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.2766
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.140609
PRIMER_LEFT_1_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_RIGHT_1_SEQUENCE=TCAATATTAGCATTGGCACACC
PRIMER_LEFT_1=69,22
PRIMER_RIGHT_1=294,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=59.859
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.2766
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.140609
PRIMER_LEFT_2_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_RIGHT_2_SEQUENCE=TCAATATTAGCATTGGCACACC
PRIMER_LEFT_2=71,22
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.859
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.4770
PRIMER_LEFT_3_PENALTY=0.336392
PRIMER_RIGHT_3_PENALTY=0.140609
PRIMER_LEFT_3_SEQUENCE=TATCCCTTGTTCCGAAAACTTG
PRIMER_RIGHT_3_SEQUENCE=TCAATATTAGCATTGGCACACC
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=60.336
PRIMER_RIGHT_3_TM=59.859
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.5476
PRIMER_LEFT_4_PENALTY=0.406964
PRIMER_RIGHT_4_PENALTY=0.140609
PRIMER_LEFT_4_SEQUENCE=TAGTGGTGGTAAGGTGGAGGAG
PRIMER_RIGHT_4_SEQUENCE=TCAATATTAGCATTGGCACACC
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=60.407
PRIMER_RIGHT_4_TM=59.859
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_02407 Ps_contig_mira-and-tgicl-ass_franssen_20485
SEQUENCE=CGTATCCCTTGTTCCGAAAACTTGGCATCTCCATATAGTGTGATGCCAATGATTGACTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTAAACCGGAGGAGGAGGAGAATGGTAAACTGGGGTAGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGCGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCCAATGCTAATATTGATTATTGGATTTGATGACAAAATCAGCTA
TARGET=229,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2766
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.140609
PRIMER_LEFT_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_RIGHT_SEQUENCE=TCAATATTAGCATTGGCACACC
PRIMER_LEFT=70,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=59.859
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.2766
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.140609
PRIMER_LEFT_1_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_RIGHT_1_SEQUENCE=TCAATATTAGCATTGGCACACC
PRIMER_LEFT_1=69,22
PRIMER_RIGHT_1=294,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=59.859
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.2766
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.140609
PRIMER_LEFT_2_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_RIGHT_2_SEQUENCE=TCAATATTAGCATTGGCACACC
PRIMER_LEFT_2=71,22
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.859
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.4273
PRIMER_LEFT_3_PENALTY=0.286660
PRIMER_RIGHT_3_PENALTY=0.140609
PRIMER_LEFT_3_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_RIGHT_3_SEQUENCE=TCAATATTAGCATTGGCACACC
PRIMER_LEFT_3=192,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=59.713
PRIMER_RIGHT_3_TM=59.859
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=0.4770
PRIMER_LEFT_4_PENALTY=0.336392
PRIMER_RIGHT_4_PENALTY=0.140609
PRIMER_LEFT_4_SEQUENCE=TATCCCTTGTTCCGAAAACTTG
PRIMER_RIGHT_4_SEQUENCE=TCAATATTAGCATTGGCACACC
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=60.336
PRIMER_RIGHT_4_TM=59.859
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_02408 Ps_contig_mira-and-tgicl-ass_franssen_20490
SEQUENCE=GKTCGGGGACACAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTCGAACCCCTTTAAACCAAAGAAGTAGTTTCTAGCTTGTTACAAACCATACGTACCAAAAACAACTACCCCATCTACAAAAGAACAAACTTACG
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02409 Ps_contig_mira-and-tgicl-ass_franssen_20490
SEQUENCE=GKTCGGGGACACAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTCGAACCCCTTTAAACCAAAGAAGTAGTTTCTAGCTTGTTACAAACCATACGTACCAAAAACAACTACCCCATCTACAAAAGAACAAACTTACG
TARGET=65,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02410 Ps_contig_mira-and-tgicl-ass_franssen_20493
SEQUENCE=TTGTCGGGGACGKAACCAAAATACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATATCAAGYAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCCACCACCGCCAACCAARASSATMCATACCATTMTCCATCCACCCGCCACCACCACCAGCTCCACCACCCTACCCTCCT
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02411 Ps_contig_mira-and-tgicl-ass_franssen_20493
SEQUENCE=TTGTCGGGGACGKAACCAAAATACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATATCAAGYAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCCACCACCGCCAACCAARASSATMCATACCATTMTCCATCCACCCGCCACCACCACCAGCTCCACCACCCTACCCTCCT
TARGET=209,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02412 Ps_contig_mira-and-tgicl-ass_franssen_20496
SEQUENCE=TTCGGGGTACTACAAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAGACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACNTCCACTTGACCCTTTAACAAGAAGTAGTTTTCAGCTGTTACCAAACCATACGTACCAAAAACAATCCCCATCTACAAACAACAG
TARGET=38,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6728
PRIMER_LEFT_PENALTY=1.577079
PRIMER_RIGHT_PENALTY=0.095684
PRIMER_LEFT_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_SEQUENCE=AACATGCAACGGTGTAACTGAG
PRIMER_LEFT=17,21
PRIMER_RIGHT=151,22
PRIMER_LEFT_TM=60.577
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=1.6728
PRIMER_LEFT_1_PENALTY=1.577079
PRIMER_RIGHT_1_PENALTY=0.095684
PRIMER_LEFT_1_SEQUENCE=AAAAACACACCAAGCCAGTGA
PRIMER_RIGHT_1_SEQUENCE=AACATGCAACGGTGTAACTGAG
PRIMER_LEFT_1=16,21
PRIMER_RIGHT_1=151,22
PRIMER_LEFT_1_TM=60.577
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=1.7017
PRIMER_LEFT_2_PENALTY=1.577079
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_2_SEQUENCE=CAACTGCATGAAATGAGAGGAG
PRIMER_LEFT_2=17,21
PRIMER_RIGHT_2=128,22
PRIMER_LEFT_2_TM=60.577
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=1.7017
PRIMER_LEFT_3_PENALTY=1.577079
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=AAAAACACACCAAGCCAGTGA
PRIMER_RIGHT_3_SEQUENCE=CAACTGCATGAAATGAGAGGAG
PRIMER_LEFT_3=16,21
PRIMER_RIGHT_3=128,22
PRIMER_LEFT_3_TM=60.577
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=1.8368
PRIMER_LEFT_4_PENALTY=1.577079
PRIMER_RIGHT_4_PENALTY=0.259704
PRIMER_LEFT_4_SEQUENCE=AAAACACACCAAGCCAGTGAA
PRIMER_RIGHT_4_SEQUENCE=ACAACTGCATGAAATGAGAGGA
PRIMER_LEFT_4=17,21
PRIMER_RIGHT_4=129,22
PRIMER_LEFT_4_TM=60.577
PRIMER_RIGHT_4_TM=59.740
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=113
=
PRIMER_SEQUENCE_ID=SSR_02413 Ps_contig_mira-and-tgicl-ass_franssen_20496
SEQUENCE=TTCGGGGTACTACAAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAGACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACNTCCACTTGACCCTTTAACAAGAAGTAGTTTTCAGCTGTTACCAAACCATACGTACCAAAAACAATCCCCATCTACAAACAACAG
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2959
PRIMER_LEFT_PENALTY=1.200250
PRIMER_RIGHT_PENALTY=0.095684
PRIMER_LEFT_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_SEQUENCE=AACATGCAACGGTGTAACTGAG
PRIMER_LEFT=18,21
PRIMER_RIGHT=151,22
PRIMER_LEFT_TM=60.200
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=1.3249
PRIMER_LEFT_1_PENALTY=1.200250
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_1_SEQUENCE=CAACTGCATGAAATGAGAGGAG
PRIMER_LEFT_1=18,21
PRIMER_RIGHT_1=128,22
PRIMER_LEFT_1_TM=60.200
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=1.4600
PRIMER_LEFT_2_PENALTY=1.200250
PRIMER_RIGHT_2_PENALTY=0.259704
PRIMER_LEFT_2_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_2_SEQUENCE=ACAACTGCATGAAATGAGAGGA
PRIMER_LEFT_2=18,21
PRIMER_RIGHT_2=129,22
PRIMER_LEFT_2_TM=60.200
PRIMER_RIGHT_2_TM=59.740
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=1.4600
PRIMER_LEFT_3_PENALTY=1.200250
PRIMER_RIGHT_3_PENALTY=0.259704
PRIMER_LEFT_3_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_3_SEQUENCE=GACAACTGCATGAAATGAGAGG
PRIMER_LEFT_3=18,21
PRIMER_RIGHT_3=130,22
PRIMER_LEFT_3_TM=60.200
PRIMER_RIGHT_3_TM=59.740
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=1.4760
PRIMER_LEFT_4_PENALTY=1.200250
PRIMER_RIGHT_4_PENALTY=0.275749
PRIMER_LEFT_4_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_4_SEQUENCE=TGTTGTTTGTAGATGGGGATTG
PRIMER_LEFT_4=18,21
PRIMER_RIGHT_4=239,22
PRIMER_LEFT_4_TM=60.200
PRIMER_RIGHT_4_TM=59.724
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_02414 Ps_contig_mira-and-tgicl-ass_franssen_20501
SEQUENCE=CGTTTACCCGCCACTGTTTAGGGTTAAACGGGTTAAGGAAGTAGCGAAGCTATGTGCATCGACGGTACAAAGACGTCTCTGTCACATCCGTGGGAAGGATGCNCAAAAAGGTACTGGATTGCCGCCGGCGATATCATACTCGTTGGTCTCCGTGATTATCAGGATGATAAGGCTGATGTTATTCTCAAATACATGCCGGATGAGGCTCGTCTTCTTAAGGCGTATGGTGAGCTTCCTGATAATACTAGGCTTAATGAAGGTATTGGTGCTGGTCTTGATGAGGAAGATGATGGTGATGCTAATGATTATATCGAGTTTGAGGATGAGGATATTGATAAATTTAGTTAAAAAAAAGTTTTTTTTGTTTGTTTGAAGTTAAAAAGTTGTAATTTACTTAAATTTAATTTATTACTATCGTTATATGTGATGA
TARGET=361,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02415 Ps_contig_mira-and-tgicl-ass_franssen_20502
SEQUENCE=CCTTTTAAAACGTAAGAAATAATTACATTATTTTTATTAATTTAAAGTTTACTACAATTANAACCATACATCACACATGGGCTGGGCCAGATTCCAACTTAAAAGTAAATGAAAAAGACTGCCCCATCACCCAACGCACTCACCACCTAATAATAATAATCATTACAACAACACGTTACACTTACAACAACATCCTTTACATACAAAAAGGGAAATAGTTAAAACAAGATAATGATCGAATTTTCAAATTAAAAATAAAACCCCACAAAGTTGGTTTGGTTTAATGGTTTTGGCATCAACTATCACAAACCGTTGGATCATATCAACTTGACTTGAAAGGAGTGGCTCTGATAACAACTGTGTGGTAAGTGCTGCAGTGCTGCACATGATGGTCCAACCAGAAATCCATTGATCACCCACCAAGATCTTGTGAGATATGCAGCACAGACTCAGCAGG
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4422
PRIMER_LEFT_PENALTY=0.343977
PRIMER_RIGHT_PENALTY=0.098207
PRIMER_LEFT_SEQUENCE=GTAAATGAAAAAGACTGCCCCA
PRIMER_RIGHT_SEQUENCE=ACTGCAGCACTTACCACACAGT
PRIMER_LEFT=104,22
PRIMER_RIGHT=377,22
PRIMER_LEFT_TM=60.344
PRIMER_RIGHT_TM=59.902
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.4768
PRIMER_LEFT_1_PENALTY=0.343977
PRIMER_RIGHT_1_PENALTY=0.132857
PRIMER_LEFT_1_SEQUENCE=GTAAATGAAAAAGACTGCCCCA
PRIMER_RIGHT_1_SEQUENCE=TGTGATAGTTGATGCCAAAACC
PRIMER_LEFT_1=104,22
PRIMER_RIGHT_1=306,22
PRIMER_LEFT_1_TM=60.344
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.4768
PRIMER_LEFT_2_PENALTY=0.343977
PRIMER_RIGHT_2_PENALTY=0.132857
PRIMER_LEFT_2_SEQUENCE=GTAAATGAAAAAGACTGCCCCA
PRIMER_RIGHT_2_SEQUENCE=GGTTTGTGATAGTTGATGCCAA
PRIMER_LEFT_2=104,22
PRIMER_RIGHT_2=310,22
PRIMER_LEFT_2_TM=60.344
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.4768
PRIMER_LEFT_3_PENALTY=0.343977
PRIMER_RIGHT_3_PENALTY=0.132857
PRIMER_LEFT_3_SEQUENCE=GTAAATGAAAAAGACTGCCCCA
PRIMER_RIGHT_3_SEQUENCE=GTGATAGTTGATGCCAAAACCA
PRIMER_LEFT_3=104,22
PRIMER_RIGHT_3=305,22
PRIMER_LEFT_3_TM=60.344
PRIMER_RIGHT_3_TM=59.867
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=202
PRIMER_PAIR_PENALTY_4=0.5210
PRIMER_LEFT_4_PENALTY=0.343977
PRIMER_RIGHT_4_PENALTY=0.177008
PRIMER_LEFT_4_SEQUENCE=GTAAATGAAAAAGACTGCCCCA
PRIMER_RIGHT_4_SEQUENCE=TCAAGTTGATATGATCCAACGG
PRIMER_LEFT_4=104,22
PRIMER_RIGHT_4=330,22
PRIMER_LEFT_4_TM=60.344
PRIMER_RIGHT_4_TM=59.823
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_02416 Ps_contig_mira-and-tgicl-ass_franssen_20528
SEQUENCE=AGTGTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTTCTTTTTCTTCACCATCCTCTCCTTCCTCTTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGGTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCACCTTGTGACCAAACTCGTGGGCAATCGCCTCATCTTGAGCAGGCTTATGTTCTTCATTCCTTTTTCTTTACCACCGAGAAAATACAAAAACAACCTACTAGTACCTTAGAATCTCTGTCTCAGAGTTGGTTGCGGAGG
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02417 Ps_contig_mira-and-tgicl-ass_franssen_20583
SEQUENCE=GTCGGGGAAACCTAAGCATTTCAACTATTTATATATATATTCACAGAGATCATATCGTATATCATAAGATGGCTGGTTTATTCAGCAACATCTTTGGTGGGTACGATGGTGGAAACAATGGAACTGTTGATGCATTTAACAACAAGTGTGGCTATACAAAGTATTTAGTGGGTTAGGAGGGTCTTGGAACTACTAACCAAAAACCGTTGGGT
TARGET=30,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1475
PRIMER_LEFT_PENALTY=1.022819
PRIMER_RIGHT_PENALTY=0.124655
PRIMER_LEFT_SEQUENCE=GGGGAAACCTAAGCATTTCAAC
PRIMER_RIGHT_SEQUENCE=TCAACAGTTCCATTGTTTCCAC
PRIMER_LEFT=3,22
PRIMER_RIGHT=129,22
PRIMER_LEFT_TM=61.023
PRIMER_RIGHT_TM=59.875
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=127
PRIMER_PAIR_PENALTY_1=1.2205
PRIMER_LEFT_1_PENALTY=1.022819
PRIMER_RIGHT_1_PENALTY=0.197640
PRIMER_LEFT_1_SEQUENCE=GGGGAAACCTAAGCATTTCAAC
PRIMER_RIGHT_1_SEQUENCE=CCCAACGGTTTTTGGTTAGTAG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=210,22
PRIMER_LEFT_1_TM=61.023
PRIMER_RIGHT_1_TM=59.802
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=1.3405
PRIMER_LEFT_2_PENALTY=1.022819
PRIMER_RIGHT_2_PENALTY=0.317700
PRIMER_LEFT_2_SEQUENCE=GGGGAAACCTAAGCATTTCAAC
PRIMER_RIGHT_2_SEQUENCE=ACCCAACGGTTTTTGGTTAGTA
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=61.023
PRIMER_RIGHT_2_TM=59.682
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=4.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=1.4232
PRIMER_LEFT_3_PENALTY=1.298500
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=GGGGAAACCTAAGCATTTCAA
PRIMER_RIGHT_3_SEQUENCE=TCAACAGTTCCATTGTTTCCAC
PRIMER_LEFT_3=3,21
PRIMER_RIGHT_3=129,22
PRIMER_LEFT_3_TM=60.299
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=1.4305
PRIMER_LEFT_4_PENALTY=1.305865
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=GGGAAACCTAAGCATTTCAACT
PRIMER_RIGHT_4_SEQUENCE=TCAACAGTTCCATTGTTTCCAC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=129,22
PRIMER_LEFT_4_TM=58.694
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_02418 Ps_contig_mira-and-tgicl-ass_franssen_20592
SEQUENCE=GGGGGGAATTACTCCTAGATAACACACACACAACACAAAAAATGGGTAAAGATGTTGAGGTTCAAGAACAAGGTGCTGAATTCTCAGCTAAGGATTACCAAGATCCACCACCAGCTCACACCTACCCTCCTCATGTT
TARGET=22,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02419 Ps_contig_mira-and-tgicl-ass_franssen_20603
SEQUENCE=GTAGACTCAAAATTTTCTGGTATATATATATGTCATGAAGAATGCAACTCTTCATCTCACATTTATTACGACAATATCATGTTATTAGAAATGTTGTGCTAATTGAAGAATGACAAAAGMYATTTGATTAGCCATGGAACCTAACAAGCTTCACATGCTAATATTACATTAATTAGGACGTAAAAATAAA
TARGET=21,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02420 Ps_contig_mira-and-tgicl-ass_franssen_20615
SEQUENCE=TCAACGCAGAGTACGCGAKSATCCAAACAACAACAACACTCTCATCATCGTCCTCATACTTTACCTTTCATTTCAGAGTTACAGCTACATAATCCATTTTAGTTA
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02421 Ps_contig_mira-and-tgicl-ass_franssen_20638
SEQUENCE=GTTAACCTAACAAGCTTCACATGCTAAGACTTTTGAGTTACAATTCAAGCAAAGATGATGAAAAYGTTACAAGTAAACAACAACCATATCTTTCTGGTAGAAGTGTACAACAAAGTAAAAAAAGMTCACAACACATAAGAGATACTGTAATCTACAACCAATTTATTACTTTTCTTTCTTTCAATTAGTCTGTCTCAACACCTAATCCATACAACACACCAGTAGTTATTTCTCTTGTTTCCGCCTAAGTTTAGGAAAACGTAACCGTTACCTTCCCCTTACTTAAATTTTTAC
TARGET=171,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02422 Ps_contig_mira-and-tgicl-ass_franssen_20681
SEQUENCE=CCTTTTTTTTAATTCCAAGGAACTTAACTTGTTGAATATCTCATACAAATAAACTATACATCATCATCATCATCATCATCATCTTAATAAACANAAAGGACAANAAAGACAAATTAAACAACATAAACGAACAACATAGTAATTCTTAATTAACCAAAGAACTAGGATCATAACATTGCTAGAAGTGG
TARGET=59,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02423 Ps_contig_mira-and-tgicl-ass_franssen_20726
SEQUENCE=ATSAACACAAAACCTAAAGCATTTCAACTATTTATATATATACACAGAGATCATATCGTATATCATAAGATGACTGGTTTTTTCAGCAACATCTTTGGTGGGTACGATGGTGGAAACAATGGAACTGTTGATGCTATTTAACAACAAGTGTGGCTANCAAGATTATGGTGAGGCTGACATACAAAACGTGGTNCAACTAAAATTACCAAGTGGGTACGGTTTAACCAACTTAATTACG
TARGET=33,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02424 Ps_contig_mira-and-tgicl-ass_franssen_20739
SEQUENCE=GATCCACACAAATATTKKGTACTAATATTATTCATTCATTCTTCTCACTATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCTCTCTCTCAATCCTATCATATTTCATTCTCTTCTCTCTCTGCAACAACCAACAGCAACAATGTCTGCTTCACTCGCCCTACCTAAAGNGCTAG
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02425 Ps_contig_mira-and-tgicl-ass_franssen_20739
SEQUENCE=GATCCACACAAATATTKKGTACTAATATTATTCATTCATTCTTCTCACTATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCTCTCTCTCAATCCTATCATATTTCATTCTCTTCTCTCTCTGCAACAACCAACAGCAACAATGTCTGCTTCACTCGCCCTACCTAAAGNGCTAG
TARGET=63,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02426 Ps_contig_mira-and-tgicl-ass_franssen_20739
SEQUENCE=GATCCACACAAATATTKKGTACTAATATTATTCATTCATTCTTCTCACTATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCTCTCTCTCAATCCTATCATATTTCATTCTCTTCTCTCTCTGCAACAACCAACAGCAACAATGTCTGCTTCACTCGCCCTACCTAAAGNGCTAG
TARGET=118,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02427 Ps_contig_mira-and-tgicl-ass_franssen_20770
SEQUENCE=CTATTAACGTGGTATATATTGGAAATTTWAAWTCAGAAACTCAAATTAAAATATGTCATTATATAGATTGATTCAACAACATAGTGAGAAACAGTGAATACAGTATAGATCTAGTACAAGAGGATAGGACCACATGTAAGATGATCCTATATCACTCTCAACTAAGTATTTTTTATTCTTAATATTTAAACTCTCATATACTAGTGACAAGTTTCCATATGACTAACCCCTGAAAACCCACCACCACCATCTTACAAAACCTAACAGTGTTACAGTT
TARGET=238,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02428 Ps_contig_mira-and-tgicl-ass_franssen_20779
SEQUENCE=AACCAAAGTAACTAACAAAGGACAGTGCAAGAGGAAAGGAATGAGCTGCCCCCACGAAAAAGACTTGCAATTGCTAATGTGGGAAGGCAGGATGCTAAGAAGCATCTTAGAAAGAAGCACGTTTCTAATTTAATGTCTTCGAACATTGTTCAGACTTTGGGAATGATGCTCGACACAGTTGTGTTCTAGAGTTAGGATCGTCCACTGTATTCTTCTTCTTCTTCATGATGTCCAGCAATTATTTCTATTTACACTTCTTTGACCTTAAAGTTTAAAAAAACC
TARGET=210,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02429 Ps_contig_mira-and-tgicl-ass_franssen_20789
SEQUENCE=ATGAATTTGTAWTATACTCAAAATGAGTCACTATTATAAATTCATTCATTCCCTTTGGATATACTATCTTGCTTCAAAACAMCACACATTTCTCTATCTCAGCAAAACAACATAGCCATGGCCACATCAATCTCTGCTGTTAATCCTATCAGCAAAACTCCATTGAGTTTGAATAATTCTCGAGCAGTAGCTTCAGCTTCAAGTTCATTCTTTCTTGGGAAGCTAAGGTTTTAAAAAGGAAGGTTTA
TARGET=40,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02430 Ps_contig_mira-and-tgicl-ass_franssen_20842
SEQUENCE=AAAGACAGTGGGTAGTCAACGCAGAGTACGTCGGGGTCGCTCTCAACTCTCCACACTTCTTCACACACTCTCTTCTCTTCACTCTCCGCCACCGATTCATCCCAAACTCCAATGGCTGTTACTTTCTCAGATCTACACACAGAACAGGGTATCAAATCCCTCGACGAATTCCTCTCCGGAAAAACCTATATCTCTGGGGATCAATTGACGAAGGATGATATCAAAGTGTATGGAGCTGTTACTGAGAAGCCAAGTGAGTCTTTCCCCAATGCTGCTAATTGGTATGATGCCGTGTCTTCAAGTCTTGCTGCTAGTTTCCCTGGAAAAGCTCAAGGTGTGAAGTTTACAGGAAGTGGTGCTGCTCCAGCTGCAGAAGCACCTGCCAAAGCAGATGCTCCCGCTGCTGAAGATGATGATGATCTTGATCTCTTCGGTGATGAAACTGAGGATGAGAAGAAGGCAGCAGAGGAAAGGGAGGCTGACTAAAAAAACCTGCCAAGAAGAAAGAAAGCGGAAAGTCTTCCATTCTGCTTGATGTTAAGCCGTTGGGATGATGAGACAGACATAGAAGAAGCGTAGGG
TARGET=409,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0911
PRIMER_LEFT_PENALTY=0.074807
PRIMER_RIGHT_PENALTY=0.016312
PRIMER_LEFT_SEQUENCE=AGTCTTGCTGCTAGTTTCCCTG
PRIMER_RIGHT_SEQUENCE=CTGCCTTCTTCTCATCCTCAGT
PRIMER_LEFT=300,22
PRIMER_RIGHT=462,22
PRIMER_LEFT_TM=60.075
PRIMER_RIGHT_TM=60.016
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.1030
PRIMER_LEFT_1_PENALTY=0.086698
PRIMER_RIGHT_1_PENALTY=0.016312
PRIMER_LEFT_1_SEQUENCE=ATGGAGCTGTTACTGAGAAGCC
PRIMER_RIGHT_1_SEQUENCE=CTGCCTTCTTCTCATCCTCAGT
PRIMER_LEFT_1=229,22
PRIMER_RIGHT_1=462,22
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=60.016
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.2309
PRIMER_LEFT_2_PENALTY=0.074807
PRIMER_RIGHT_2_PENALTY=0.156135
PRIMER_LEFT_2_SEQUENCE=AGTCTTGCTGCTAGTTTCCCTG
PRIMER_RIGHT_2_SEQUENCE=ACGGCTTAACATCAAGCAGAAT
PRIMER_LEFT_2=300,22
PRIMER_RIGHT_2=545,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=60.156
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=0.2458
PRIMER_LEFT_3_PENALTY=0.229480
PRIMER_RIGHT_3_PENALTY=0.016312
PRIMER_LEFT_3_SEQUENCE=TAGTTTCCCTGGAAAAGCTCAA
PRIMER_RIGHT_3_SEQUENCE=CTGCCTTCTTCTCATCCTCAGT
PRIMER_LEFT_3=311,22
PRIMER_RIGHT_3=462,22
PRIMER_LEFT_3_TM=60.229
PRIMER_RIGHT_3_TM=60.016
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=0.3117
PRIMER_LEFT_4_PENALTY=0.295392
PRIMER_RIGHT_4_PENALTY=0.016312
PRIMER_LEFT_4_SEQUENCE=AATTCCTCTCCGGAAAAACCTA
PRIMER_RIGHT_4_SEQUENCE=CTGCCTTCTTCTCATCCTCAGT
PRIMER_LEFT_4=166,22
PRIMER_RIGHT_4=462,22
PRIMER_LEFT_4_TM=60.295
PRIMER_RIGHT_4_TM=60.016
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_02431 Ps_contig_mira-and-tgicl-ass_franssen_20848
SEQUENCE=TGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTCAAGTGAAGAGGAAGGGAGAAAGATGGGTGAGAAAAGAAAGAAGAAGAAGGAAANAGAGAAGAAGACACTCGTGCNTGNGAAGTGAGTGTGAGANACA
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9189
PRIMER_LEFT_PENALTY=0.117452
PRIMER_RIGHT_PENALTY=0.801493
PRIMER_LEFT_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_RIGHT_SEQUENCE=CANGCACGAGTGTCTTCTTCTC
PRIMER_LEFT=1,22
PRIMER_RIGHT=116,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=60.801
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=1.1452
PRIMER_LEFT_1_PENALTY=0.117452
PRIMER_RIGHT_1_PENALTY=1.027792
PRIMER_LEFT_1_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_RIGHT_1_SEQUENCE=ANGCACGAGTGTCTTCTTCTCT
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=115,22
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=58.972
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=1.4686
PRIMER_LEFT_2_PENALTY=0.667126
PRIMER_RIGHT_2_PENALTY=0.801493
PRIMER_LEFT_2_SEQUENCE=GAGAAACTTCACCGATCTGACA
PRIMER_RIGHT_2_SEQUENCE=CANGCACGAGTGTCTTCTTCTC
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=116,22
PRIMER_LEFT_2_TM=59.333
PRIMER_RIGHT_2_TM=60.801
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=115
PRIMER_PAIR_PENALTY_3=1.6949
PRIMER_LEFT_3_PENALTY=0.667126
PRIMER_RIGHT_3_PENALTY=1.027792
PRIMER_LEFT_3_SEQUENCE=GAGAAACTTCACCGATCTGACA
PRIMER_RIGHT_3_SEQUENCE=ANGCACGAGTGTCTTCTTCTCT
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=115,22
PRIMER_LEFT_3_TM=59.333
PRIMER_RIGHT_3_TM=58.972
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=1.7346
PRIMER_LEFT_4_PENALTY=0.933135
PRIMER_RIGHT_4_PENALTY=0.801493
PRIMER_LEFT_4_SEQUENCE=ATCTGACAGCTCATCAAGCTCC
PRIMER_RIGHT_4_SEQUENCE=CANGCACGAGTGTCTTCTTCTC
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=116,22
PRIMER_LEFT_4_TM=60.933
PRIMER_RIGHT_4_TM=60.801
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_02432 Ps_contig_mira-and-tgicl-ass_franssen_20866
SEQUENCE=CGTTCCTACCTCGATTCTAACTGTTTTGTTTCTTCAATNACANCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTTGCTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTTCACTTCTTCAACCGATTTCCTCAGTTTCTTTAGGTGCTTCTAAGTTCTCTTCCTTTGCTTACCTAACTGTTACCACTTCCTTGTTCTCAACTTCAAGTGGATCTTGGGTTTCGGGAGCATCTACAGCTTCTCCTGCTGCAGTTGTTTCTGTTTGGTTTTTCGGTTGTTTCTTCTGTTGGTACTTCGGTGGCAGGTAACTTCGGATTTCGTGGTTGTGCCGCAGCGAGGGTACTTCGAGGTGGTCGTTGGGTTGTCCGTTTTGGTTTTTTAGTTAACG
TARGET=56,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6680
PRIMER_LEFT_PENALTY=1.665678
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=CGTTCCTACCTCGATTCTAACTG
PRIMER_RIGHT_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_LEFT=0,23
PRIMER_RIGHT=137,22
PRIMER_LEFT_TM=59.334
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=138
PRIMER_PAIR_PENALTY_1=1.7752
PRIMER_LEFT_1_PENALTY=1.665678
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=CGTTCCTACCTCGATTCTAACTG
PRIMER_RIGHT_1_SEQUENCE=GAGGAAATCGGTTGAAGAAGTG
PRIMER_LEFT_1=0,23
PRIMER_RIGHT_1=148,22
PRIMER_LEFT_1_TM=59.334
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=1.7752
PRIMER_LEFT_2_PENALTY=1.665678
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=CGTTCCTACCTCGATTCTAACTG
PRIMER_RIGHT_2_SEQUENCE=AACTGAGGAAATCGGTTGAAGA
PRIMER_LEFT_2=0,23
PRIMER_RIGHT_2=152,22
PRIMER_LEFT_2_TM=59.334
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=153
PRIMER_PAIR_PENALTY_3=1.7752
PRIMER_LEFT_3_PENALTY=1.665678
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=CGTTCCTACCTCGATTCTAACTG
PRIMER_RIGHT_3_SEQUENCE=TGAGGAAATCGGTTGAAGAAGT
PRIMER_LEFT_3=0,23
PRIMER_RIGHT_3=149,22
PRIMER_LEFT_3_TM=59.334
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=150
PRIMER_PAIR_PENALTY_4=1.7752
PRIMER_LEFT_4_PENALTY=1.665678
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=CGTTCCTACCTCGATTCTAACTG
PRIMER_RIGHT_4_SEQUENCE=ACTGAGGAAATCGGTTGAAGAA
PRIMER_LEFT_4=0,23
PRIMER_RIGHT_4=151,22
PRIMER_LEFT_4_TM=59.334
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_02433 Ps_contig_mira-and-tgicl-ass_franssen_20872
SEQUENCE=GCAATCGCGTGGAGAACAAGGTGTATCAATTTCTTCACTTCCTTCTTCAAGGGAAGAGATTTGTAACTTATAATCGCTTCACCTCCAACTATTATTAAGCCAATAAGCATAAGAACAGGATGAAGATTGAAGATGAGAGCCTTGTTGGTAGAACTCCAAGCCAAACCACCTCTGAAATGGAAGTTCCAGACCAACACCAACACGGCGGCGACAACACCGAGAAGGTGAACAACGTACGTGAACGGAACGGCGGGTACACCCAACGCCATCTTCAACGGTTATTGGTGTTTGTTGTTGTTGTTGGGAGAAGATCCTGGGAAGAAAAAGGCGCACGGGAGACACGAGTTTCGTCGGTGTAGTTGTTAAGGTATATACGGTGTGTCGAAGTACAGGTCATCTGACTGCCCTTCTAGCCC
TARGET=289,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4479
PRIMER_LEFT_PENALTY=0.039879
PRIMER_RIGHT_PENALTY=0.408061
PRIMER_LEFT_SEQUENCE=GTAGAACTCCAAGCCAAACCAC
PRIMER_RIGHT_SEQUENCE=CCTTTTTCTTCCCAGGATCTTC
PRIMER_LEFT=147,22
PRIMER_RIGHT=327,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=60.408
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.4551
PRIMER_LEFT_1_PENALTY=0.039879
PRIMER_RIGHT_1_PENALTY=0.415192
PRIMER_LEFT_1_SEQUENCE=GTAGAACTCCAAGCCAAACCAC
PRIMER_RIGHT_1_SEQUENCE=ACCTGTACTTCGACACACCGTA
PRIMER_LEFT_1=147,22
PRIMER_RIGHT_1=393,22
PRIMER_LEFT_1_TM=60.040
PRIMER_RIGHT_1_TM=59.585
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.4821
PRIMER_LEFT_2_PENALTY=0.073989
PRIMER_RIGHT_2_PENALTY=0.408061
PRIMER_LEFT_2_SEQUENCE=GAAGGTGAACAACGTACGTGAA
PRIMER_RIGHT_2_SEQUENCE=CCTTTTTCTTCCCAGGATCTTC
PRIMER_LEFT_2=220,22
PRIMER_RIGHT_2=327,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=60.408
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.4892
PRIMER_LEFT_3_PENALTY=0.073989
PRIMER_RIGHT_3_PENALTY=0.415192
PRIMER_LEFT_3_SEQUENCE=GAAGGTGAACAACGTACGTGAA
PRIMER_RIGHT_3_SEQUENCE=ACCTGTACTTCGACACACCGTA
PRIMER_LEFT_3=220,22
PRIMER_RIGHT_3=393,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.585
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.5502
PRIMER_LEFT_4_PENALTY=0.142170
PRIMER_RIGHT_4_PENALTY=0.408061
PRIMER_LEFT_4_SEQUENCE=TCTTCACTTCCTTCTTCAAGGG
PRIMER_RIGHT_4_SEQUENCE=CCTTTTTCTTCCCAGGATCTTC
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=327,22
PRIMER_LEFT_4_TM=59.858
PRIMER_RIGHT_4_TM=60.408
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_02434 Ps_contig_mira-and-tgicl-ass_franssen_20877
SEQUENCE=GGAAGTTTAGGTAGGAAAAMSAAAAGAAAACAAAAAGAAAGGGACCGATGAAATTAGTAGATATCCATGTTTGTGTTTCAAGGGTGTAATTTGTATCCCTTTTGTGTCTTTTTATTTTTATTTATTATTTCCTATGTATGTATGCGATTCCTATGTGTTTGTTTTTCTATTTTGTGTATCATTAGTTTATTTTCAAGTTCTCTTTCATTATTAAGTGCCATTATTTTCCAAACGT
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02435 Ps_contig_mira-and-tgicl-ass_franssen_20889
SEQUENCE=ATACTTTTCTTTCTTTCAATTAGTCTGTCTCRACACCTAATCCATACAACACACCAGAGTATTTCTCTGTTTCGCCATTGAAGAACGTATCCCTCAATTTTCTAAATGCACAAGCAGTGAAGTAAACTATCCGATCCTGCTTGATGGACAAACACCAATCCTTCAACTTCCAACACTCTGCGTTGAMTACCCACTTGTCCTT
TARGET=6,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02436 Ps_contig_mira-and-tgicl-ass_franssen_20905
SEQUENCE=CCTCCTCGTGTCGTCGTCGTCTWCCTCCCGTTCGTTCGGTCGGTCGTTCTACGTAAGAAGACGWGGTAGGTCTACGACACGAGATACGGGTCGGGTCGTCTCTCTCTCTCTCTCTCCACCACACAGAATTTGAGCAGAGAGATAGTGCCAGCGGAACCATGGCTTCTTCCACCAACATCCGTGAAAACTTCGTCTTTATTGCCAAGCTAGCAGAACAAGCTGAACGCTATGATGAAATGGTGGATGCAATGAAGAAGCTAGCGAAGATGGATGTTGAATTGAGTGTGGAAGAGAGAAACTTGTTTTCTGTTGGGTACAAGAATGTGGTGGGGTCAAGGAGAGCTTCATGGAGGATCCTATCATCAATTGAGCAGAAAGAGGAATCAAAAGGGAATGAGTTGAATGTTAAGCGTATTAAGGGTTACAGACAGAAGGTGGAGGTGGAACTTTCCGACATTTGCAATGACATTATGATTATTTTGGATGAGCATCTTATTCCTTCCACTAACATTGCTGAGTCCACAGTGTTCTATTATAAGATGAAAGGAGATTATCATCGGTATTTAGCTGAATTCAAATCCGGTGATGAAAAGAAAGAGGTAGCAGATCAGTCACTTAAAGCATATCAGAGTGCTTCTGCGACTGCGGAGAATGAATTACAACCTACGCATCCTATCCGATTGGGTCTTGCTCTAAATTTCTCAGTGTTTTATTATGAGATAATGAATTCACCTGAAAGGGCCTGCCACCTTGCAAAACAAGCGTTTGACGACGGTGTCTCGGAGTTGGATAGCCTAAATGAAGATTCTTACAAGGACAGTACCTTGATCATGCAGCTGTTGCGAGACAACCTTACTTTATGGACTTCTGATATCCCAGAAGATAGTGAGGACCAGAAAATGGAAAGCGCGACTAAGAGTGGTCAAGAAGAAGAAGAATTGGGCCGCTAAGTTATCGTTCTGTCAAGTAGTTATCATCCGGTGAACATTTTCATCTTTGAGTTATTGTTAAATTTAGAGTTGCATCATTTTATTTGAATCATGTGTTTCATTTGTTTGCTATTGTTGTTGTATCTGTTTGTGTAACAAAAACATCATTGGTTTGTAATTCATGATTCCTCAGTTTGCATTCTGWACCTCAATTATTTAGGATATGCTTTATGTTGGATCATTT
TARGET=10,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02437 Ps_contig_mira-and-tgicl-ass_franssen_20905
SEQUENCE=CCTCCTCGTGTCGTCGTCGTCTWCCTCCCGTTCGTTCGGTCGGTCGTTCTACGTAAGAAGACGWGGTAGGTCTACGACACGAGATACGGGTCGGGTCGTCTCTCTCTCTCTCTCTCCACCACACAGAATTTGAGCAGAGAGATAGTGCCAGCGGAACCATGGCTTCTTCCACCAACATCCGTGAAAACTTCGTCTTTATTGCCAAGCTAGCAGAACAAGCTGAACGCTATGATGAAATGGTGGATGCAATGAAGAAGCTAGCGAAGATGGATGTTGAATTGAGTGTGGAAGAGAGAAACTTGTTTTCTGTTGGGTACAAGAATGTGGTGGGGTCAAGGAGAGCTTCATGGAGGATCCTATCATCAATTGAGCAGAAAGAGGAATCAAAAGGGAATGAGTTGAATGTTAAGCGTATTAAGGGTTACAGACAGAAGGTGGAGGTGGAACTTTCCGACATTTGCAATGACATTATGATTATTTTGGATGAGCATCTTATTCCTTCCACTAACATTGCTGAGTCCACAGTGTTCTATTATAAGATGAAAGGAGATTATCATCGGTATTTAGCTGAATTCAAATCCGGTGATGAAAAGAAAGAGGTAGCAGATCAGTCACTTAAAGCATATCAGAGTGCTTCTGCGACTGCGGAGAATGAATTACAACCTACGCATCCTATCCGATTGGGTCTTGCTCTAAATTTCTCAGTGTTTTATTATGAGATAATGAATTCACCTGAAAGGGCCTGCCACCTTGCAAAACAAGCGTTTGACGACGGTGTCTCGGAGTTGGATAGCCTAAATGAAGATTCTTACAAGGACAGTACCTTGATCATGCAGCTGTTGCGAGACAACCTTACTTTATGGACTTCTGATATCCCAGAAGATAGTGAGGACCAGAAAATGGAAAGCGCGACTAAGAGTGGTCAAGAAGAAGAAGAATTGGGCCGCTAAGTTATCGTTCTGTCAAGTAGTTATCATCCGGTGAACATTTTCATCTTTGAGTTATTGTTAAATTTAGAGTTGCATCATTTTATTTGAATCATGTGTTTCATTTGTTTGCTATTGTTGTTGTATCTGTTTGTGTAACAAAAACATCATTGGTTTGTAATTCATGATTCCTCAGTTTGCATTCTGWACCTCAATTATTTAGGATATGCTTTATGTTGGATCATTT
TARGET=99,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02438 Ps_contig_mira-and-tgicl-ass_franssen_20905
SEQUENCE=CCTCCTCGTGTCGTCGTCGTCTWCCTCCCGTTCGTTCGGTCGGTCGTTCTACGTAAGAAGACGWGGTAGGTCTACGACACGAGATACGGGTCGGGTCGTCTCTCTCTCTCTCTCTCCACCACACAGAATTTGAGCAGAGAGATAGTGCCAGCGGAACCATGGCTTCTTCCACCAACATCCGTGAAAACTTCGTCTTTATTGCCAAGCTAGCAGAACAAGCTGAACGCTATGATGAAATGGTGGATGCAATGAAGAAGCTAGCGAAGATGGATGTTGAATTGAGTGTGGAAGAGAGAAACTTGTTTTCTGTTGGGTACAAGAATGTGGTGGGGTCAAGGAGAGCTTCATGGAGGATCCTATCATCAATTGAGCAGAAAGAGGAATCAAAAGGGAATGAGTTGAATGTTAAGCGTATTAAGGGTTACAGACAGAAGGTGGAGGTGGAACTTTCCGACATTTGCAATGACATTATGATTATTTTGGATGAGCATCTTATTCCTTCCACTAACATTGCTGAGTCCACAGTGTTCTATTATAAGATGAAAGGAGATTATCATCGGTATTTAGCTGAATTCAAATCCGGTGATGAAAAGAAAGAGGTAGCAGATCAGTCACTTAAAGCATATCAGAGTGCTTCTGCGACTGCGGAGAATGAATTACAACCTACGCATCCTATCCGATTGGGTCTTGCTCTAAATTTCTCAGTGTTTTATTATGAGATAATGAATTCACCTGAAAGGGCCTGCCACCTTGCAAAACAAGCGTTTGACGACGGTGTCTCGGAGTTGGATAGCCTAAATGAAGATTCTTACAAGGACAGTACCTTGATCATGCAGCTGTTGCGAGACAACCTTACTTTATGGACTTCTGATATCCCAGAAGATAGTGAGGACCAGAAAATGGAAAGCGCGACTAAGAGTGGTCAAGAAGAAGAAGAATTGGGCCGCTAAGTTATCGTTCTGTCAAGTAGTTATCATCCGGTGAACATTTTCATCTTTGAGTTATTGTTAAATTTAGAGTTGCATCATTTTATTTGAATCATGTGTTTCATTTGTTTGCTATTGTTGTTGTATCTGTTTGTGTAACAAAAACATCATTGGTTTGTAATTCATGATTCCTCAGTTTGCATTCTGWACCTCAATTATTTAGGATATGCTTTATGTTGGATCATTT
TARGET=925,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02439 Ps_contig_mira-and-tgicl-ass_franssen_20909
SEQUENCE=GGGGKAWCWACAYAAACCTAAGCATTTCAACTATTTATATATATATTCACAGAGATCATATCGTATATCATAAGATGACTGGTTTATTCAGCAACATTCGTTTGGGTGGGTACGATGGTAGGAAACAATGGAACTGTTGATGACTAATTTAACAACAAGTGTGGCTATMMAAGTATTTAGTGGGTGGAGGCTGACATAACAAACGTGGT
TARGET=36,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02440 Ps_contig_mira-and-tgicl-ass_franssen_20988
SEQUENCE=TCGGGTATTCCAAACAACAACAACACTCTCATCATCGTCTCATACTTTACCTTTCTATTTCAAGAGTTACAGCTACATAATCCATTTTAGTTATAYWTWATTMATTTCCAGTTCGAATCCATCTTGGTTAGTAGTGATCATCATGGCTAGAAGGAAGAATCAGAAACAAGTACGAGGAGACCTACATCCGCAACCAACTCTGAGACAGAGGTCAAAGGACAGTGGTTGTTTTTGATTTTCG
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02441 Ps_contig_mira-and-tgicl-ass_franssen_21023
SEQUENCE=AAACAATGTAAAACTAAGTTTCTAAGGCGTCCCGGTTCGGCATGTTCTCACACTCTTCTCGAATTTYYTGGAAAAGAACAGTTGACAGATATCTTTCGCCAAAGCKCGGGAATACAACCGCAATAAAGACTTTACCCTTAATTTTCAGGTCTCTTAGCCACCTGCAATGCTRCAGCAGCAGCAGCACCTGAAGAAATTCCCACCAGCAAGCCTTCTTTGAGTGCTATTTGCTTTTGTAGTTTCAATAGCTACATCACTAGATATCGCTATAACTTCATCCAACCTACTTCTTCACTACCAAACGTTCCCGGGACAACCAAAACCACCG
TARGET=173,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02442 Ps_contig_mira-and-tgicl-ass_franssen_21034
SEQUENCE=GGATGTCAAATAACTTTGTAATAAAAAACAATCTATTGCAAACAGGTCAAAGAGTGTAAATGAAATATTGCTGGACATCATGAAGAAGAAGAAGAATACAGTGGACGATCCTAACTCTAGAACACAACTGTGTCGANCATCATTCCCAAAGTCTGAACATGTACGAAGACTATTAAATTAGAACGTNCTCTTCAAGATTGTCCTTTCTTAGCCTACTCCCTCGTCCCCTTACCC
TARGET=82,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2100
PRIMER_LEFT_PENALTY=0.180765
PRIMER_RIGHT_PENALTY=0.029253
PRIMER_LEFT_SEQUENCE=TTGCAAACAGGTCAAAGAGTGT
PRIMER_RIGHT_SEQUENCE=TGTTCAGACTTTGGGAATGATG
PRIMER_LEFT=35,22
PRIMER_RIGHT=158,22
PRIMER_LEFT_TM=59.819
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=0.2100
PRIMER_LEFT_1_PENALTY=0.180765
PRIMER_RIGHT_1_PENALTY=0.029253
PRIMER_LEFT_1_SEQUENCE=TTGCAAACAGGTCAAAGAGTGT
PRIMER_RIGHT_1_SEQUENCE=CATGTTCAGACTTTGGGAATGA
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=160,22
PRIMER_LEFT_1_TM=59.819
PRIMER_RIGHT_1_TM=59.971
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.2110
PRIMER_LEFT_2_PENALTY=0.180765
PRIMER_RIGHT_2_PENALTY=0.030276
PRIMER_LEFT_2_SEQUENCE=TTGCAAACAGGTCAAAGAGTGT
PRIMER_RIGHT_2_SEQUENCE=TCGTACATGTTCAGACTTTGGG
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=165,22
PRIMER_LEFT_2_TM=59.819
PRIMER_RIGHT_2_TM=60.030
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=131
PRIMER_PAIR_PENALTY_3=0.2110
PRIMER_LEFT_3_PENALTY=0.180765
PRIMER_RIGHT_3_PENALTY=0.030276
PRIMER_LEFT_3_SEQUENCE=TTGCAAACAGGTCAAAGAGTGT
PRIMER_RIGHT_3_SEQUENCE=CGTACATGTTCAGACTTTGGGA
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=164,22
PRIMER_LEFT_3_TM=59.819
PRIMER_RIGHT_3_TM=60.030
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=130
PRIMER_PAIR_PENALTY_4=0.2912
PRIMER_LEFT_4_PENALTY=0.180765
PRIMER_RIGHT_4_PENALTY=0.110392
PRIMER_LEFT_4_SEQUENCE=TTGCAAACAGGTCAAAGAGTGT
PRIMER_RIGHT_4_SEQUENCE=TCAGACTTTGGGAATGATGNTC
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=155,22
PRIMER_LEFT_4_TM=59.819
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.50
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.3500
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_02443 Ps_contig_mira-and-tgicl-ass_franssen_21056
SEQUENCE=GTAAAAAAAGACTCACAACACATAAGAGATACTGTAATCTAGYAACCAATTATTACTTTTCTTTCTTTCAATTAGTCTGTCTCAACACCTAATCCATACAACACACCAGAGTATTTCTCTGTTTCGCCATTGAAGAACGTATCCCTCAATTTTCTAAATGCACAAGCAGTGAGTAAACTATCCGATCCTGCTTGATGGCAAACACCATCCTTTCAACTTCCAACACTCTGCGTTGATACCATGCTT
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02444 Ps_contig_mira-and-tgicl-ass_franssen_21067
SEQUENCE=CATGGTGGTGGAGGTTACGGTCATGGTGGTTGGTAGGTTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGCGGTGGACACTATGGTGGTGGACACTATGGGAGGTGGACACTATGGGTGGTGGTCACTACGGTCATGGGTGGTGGTCATCATGGGTGGACAGTGGTGG
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8098
PRIMER_LEFT_PENALTY=0.513831
PRIMER_RIGHT_PENALTY=0.295944
PRIMER_LEFT_SEQUENCE=TGGTTGGTAGGTTTACGGTCAT
PRIMER_RIGHT_SEQUENCE=AGTGTCCACCTCCCATAGTGTC
PRIMER_LEFT=26,22
PRIMER_RIGHT=223,22
PRIMER_LEFT_TM=60.514
PRIMER_RIGHT_TM=60.296
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=0.8098
PRIMER_LEFT_1_PENALTY=0.513831
PRIMER_RIGHT_1_PENALTY=0.295944
PRIMER_LEFT_1_SEQUENCE=GTTGGTAGGTTTACGGTCATGG
PRIMER_RIGHT_1_SEQUENCE=AGTGTCCACCTCCCATAGTGTC
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=223,22
PRIMER_LEFT_1_TM=60.514
PRIMER_RIGHT_1_TM=60.296
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=1.1347
PRIMER_LEFT_2_PENALTY=0.513831
PRIMER_RIGHT_2_PENALTY=0.620879
PRIMER_LEFT_2_SEQUENCE=TGGTTGGTAGGTTTACGGTCAT
PRIMER_RIGHT_2_SEQUENCE=CCATAGTGTCCACCTCCCATAG
PRIMER_LEFT_2=26,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=60.514
PRIMER_RIGHT_2_TM=60.621
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=1.1357
PRIMER_LEFT_3_PENALTY=0.513831
PRIMER_RIGHT_3_PENALTY=0.621889
PRIMER_LEFT_3_SEQUENCE=TGGTTGGTAGGTTTACGGTCAT
PRIMER_RIGHT_3_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=147,22
PRIMER_LEFT_3_TM=60.514
PRIMER_RIGHT_3_TM=59.378
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=1.1357
PRIMER_LEFT_4_PENALTY=0.513831
PRIMER_RIGHT_4_PENALTY=0.621889
PRIMER_LEFT_4_SEQUENCE=GTTGGTAGGTTTACGGTCATGG
PRIMER_RIGHT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=147,22
PRIMER_LEFT_4_TM=60.514
PRIMER_RIGHT_4_TM=59.378
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_02445 Ps_contig_mira-and-tgicl-ass_franssen_21067
SEQUENCE=CATGGTGGTGGAGGTTACGGTCATGGTGGTTGGTAGGTTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGCGGTGGACACTATGGTGGTGGACACTATGGGAGGTGGACACTATGGGTGGTGGTCACTACGGTCATGGGTGGTGGTCATCATGGGTGGACAGTGGTGG
TARGET=76,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8098
PRIMER_LEFT_PENALTY=0.513831
PRIMER_RIGHT_PENALTY=0.295944
PRIMER_LEFT_SEQUENCE=TGGTTGGTAGGTTTACGGTCAT
PRIMER_RIGHT_SEQUENCE=AGTGTCCACCTCCCATAGTGTC
PRIMER_LEFT=26,22
PRIMER_RIGHT=223,22
PRIMER_LEFT_TM=60.514
PRIMER_RIGHT_TM=60.296
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=0.8098
PRIMER_LEFT_1_PENALTY=0.513831
PRIMER_RIGHT_1_PENALTY=0.295944
PRIMER_LEFT_1_SEQUENCE=GTTGGTAGGTTTACGGTCATGG
PRIMER_RIGHT_1_SEQUENCE=AGTGTCCACCTCCCATAGTGTC
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=223,22
PRIMER_LEFT_1_TM=60.514
PRIMER_RIGHT_1_TM=60.296
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=1.1347
PRIMER_LEFT_2_PENALTY=0.513831
PRIMER_RIGHT_2_PENALTY=0.620879
PRIMER_LEFT_2_SEQUENCE=TGGTTGGTAGGTTTACGGTCAT
PRIMER_RIGHT_2_SEQUENCE=CCATAGTGTCCACCTCCCATAG
PRIMER_LEFT_2=26,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=60.514
PRIMER_RIGHT_2_TM=60.621
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=1.1357
PRIMER_LEFT_3_PENALTY=0.513831
PRIMER_RIGHT_3_PENALTY=0.621889
PRIMER_LEFT_3_SEQUENCE=TGGTTGGTAGGTTTACGGTCAT
PRIMER_RIGHT_3_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=147,22
PRIMER_LEFT_3_TM=60.514
PRIMER_RIGHT_3_TM=59.378
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=1.1357
PRIMER_LEFT_4_PENALTY=0.513831
PRIMER_RIGHT_4_PENALTY=0.621889
PRIMER_LEFT_4_SEQUENCE=GTTGGTAGGTTTACGGTCATGG
PRIMER_RIGHT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=147,22
PRIMER_LEFT_4_TM=60.514
PRIMER_RIGHT_4_TM=59.378
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_02446 Ps_contig_mira-and-tgicl-ass_franssen_21068
SEQUENCE=AATTTTTTTTAATTCCATGTTGTTGTGTCTATAACATATATAATAATTTGTCAAAGCACTTACGGATCTCAAGACTTCAACATCTGCAGATAAATGCATGTAAGTTACAAGAAAAATCCTGTAAATACATAGTCAAACAGCTCATTTTATTTCCCTTTTGACAAATTTGATAACTATAAAACACGAGTTCTACTTTTACAGCTTTAATCAAACATTGAATAACTAAGATTCAGTTGATGTGGTTGCATCGTCTTTGTGCTTGATCATACCAGAATCCACGAGAATGGGAATCTCCGCTGCAAGCCACGGGGCAAGACCCAAGCAAAGTGCATGTGCTATACCGAATCTAGGGCAGTTTTTTGCCCAGAGAGGCTTGAAGTGCACAGTCAAACAAATCTTTCCTCCTCTATACATCTTCTGAGTTTTTCCATCCAATTGAGGGAGTTCAAGCTCCGGCGCGGTGGAAGGGTAAGTAACCGGAATATCGAACTGAAGATCAAATTCATACTTGAGAAGATTATAAACATACCAACATTTACCGGTCCAGCGAGTACCTTCGGGATTGGAAGAGGAGATCCGAAACCAATCGTTGTCGTTGGATTTGTTCATCTGAGTGTATGCGATGAGAGATTTGTACTCTTCCTTCAGCCTCGTCGTCCATGCCGCTCCGTCTCGAGGACCGGCCTTTGTTGTTAACAGTGGGATCTGTGTTAGCGTCGACTTCGTATTCGGGTCCCAACCTTCCATCGCTCTCTCTGTTTTCTATTCTCTTCTTTTCGCTTTCAACGCACGCGCTTAGTTATGCTGCATCCCTTGTCTACACTCTCCCTAGTGGTTTCACAATCGAACCGGTTTAACGTTTCAACTATGATATATATTTATTTATTTTAAGGTTACACTTTAAATTCGATMGGGCTTCTTTCTTATCGTAAGAATTTATAAGTGTCTCTCATGTTTCAAAATCCCCGAC
TARGET=876,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02447 Ps_contig_mira-and-tgicl-ass_franssen_21079
SEQUENCE=TCCACCTTCACGACGGCCACCATAGCCTCCACCTCCACCATATCCACCACCACCACGTCCACCTCCATATCCACCGCCACCACCACGGCCGCCTCCACCGCTACCACGGCTCTGAGCTTCGTTTACTGTGATGTTACGTCCATCCATGTCCTGACCGTTCATCCCTTCGATCGCATCTCTCATGGATTGCTCCGTGGCAAAGGTCACGAATCCAAAACCTCTTGACCTTCCAGTCTCACGATCGTTAATGATCTATTGGTGTGAGCAATAAAAGAATCATATGATATAGTAACAGAATCAGTAACAGAATCACAGTAGAAAATAGATCAAGTAACACAGTAACAGAACAGATCACAGATCAGAATAGGATCGAGATGAATCCGAATCAAAATCGTGTCAGATCCGCCTGCAACAAACGGACCTTCGAGTCGACGATTTCACCGTATTGAGAGAAAGCTTTCTCTAGGGCATCGCTGTCAGTGGCCCATGCTAGACCACCAACGAAGCACCGGTACTCAACATCTGAGGAAGCCATGGAAGAAACACTGAAATTGGGACTGAGATGGTGTAAAGGCGAAAGATTGCTTGAAGATCCCCGAC
TARGET=44,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0057
PRIMER_LEFT_PENALTY=1.717979
PRIMER_RIGHT_PENALTY=0.287765
PRIMER_LEFT_SEQUENCE=ATAGCCTCCACCTCCACCATA
PRIMER_RIGHT_SEQUENCE=GTGAGACTGGAAGGTCAAGAGG
PRIMER_LEFT=21,21
PRIMER_RIGHT=238,22
PRIMER_LEFT_TM=60.718
PRIMER_RIGHT_TM=60.288
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=2.0057
PRIMER_LEFT_1_PENALTY=1.717979
PRIMER_RIGHT_1_PENALTY=0.287765
PRIMER_LEFT_1_SEQUENCE=ATAGCCTCCACCTCCACCATA
PRIMER_RIGHT_1_SEQUENCE=TGAGACTGGAAGGTCAAGAGGT
PRIMER_LEFT_1=21,21
PRIMER_RIGHT_1=237,22
PRIMER_LEFT_1_TM=60.718
PRIMER_RIGHT_1_TM=60.288
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=2.0169
PRIMER_LEFT_2_PENALTY=1.717979
PRIMER_RIGHT_2_PENALTY=0.298898
PRIMER_LEFT_2_SEQUENCE=ATAGCCTCCACCTCCACCATA
PRIMER_RIGHT_2_SEQUENCE=TGGATGGACGTAACATCACAGT
PRIMER_LEFT_2=21,21
PRIMER_RIGHT_2=145,22
PRIMER_LEFT_2_TM=60.718
PRIMER_RIGHT_2_TM=60.299
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=2.3987
PRIMER_LEFT_3_PENALTY=1.717979
PRIMER_RIGHT_3_PENALTY=0.680708
PRIMER_LEFT_3_SEQUENCE=ATAGCCTCCACCTCCACCATA
PRIMER_RIGHT_3_SEQUENCE=ATCGTGAGACTGGAAGGTCAAG
PRIMER_LEFT_3=21,21
PRIMER_RIGHT_3=241,22
PRIMER_LEFT_3_TM=60.718
PRIMER_RIGHT_3_TM=60.681
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=2.4122
PRIMER_LEFT_4_PENALTY=1.717979
PRIMER_RIGHT_4_PENALTY=0.694262
PRIMER_LEFT_4_SEQUENCE=ATAGCCTCCACCTCCACCATA
PRIMER_RIGHT_4_SEQUENCE=GGAAGGTCAAGAGGTTTTGGAT
PRIMER_LEFT_4=21,21
PRIMER_RIGHT_4=230,22
PRIMER_LEFT_4_TM=60.718
PRIMER_RIGHT_4_TM=60.694
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_02448 Ps_contig_mira-and-tgicl-ass_franssen_21090
SEQUENCE=GCATTCCATTCCATTCACTCATCAAACCAAAAACAATGGCAGCATCAGCATCATCCATGATCTCAACAAACGCCCTAACATCCACACTCCAAAAACACCAAMCACACAACCTCAAACCATCAAACCTATGTTTCCAAGGCTTCAAACCCTWASMAGATCAAMMAAACTGACACAGAAACAAAAAGAAGATTCTCATCCCTTGTTGTCAAAGCTGAACTACCTAACCCTTA
TARGET=2,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02449 Ps_contig_mira-and-tgicl-ass_franssen_21096
SEQUENCE=ACTTAGAAGCACCTAAAGAAACTGAGGAATCGGKTTGTAAGAAGTGAAGGAAGAGGCGGAAGCAGTAGTGGAGGAGCCAATAGCAACTACTGAAAATGAGTCAGCACCACCACCACCAGCAACTGCAGAGGATGTGATTGAGAAMMAAAACAGTTGAATCAGTGGAAGCTCCAGCAGCAGAGGAATTCCAGTTGAGAACTCCTAGAACGTACCTTAAAACCAAGTGGAAGTTGGGTGT
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02450 Ps_contig_mira-and-tgicl-ass_franssen_21108
SEQUENCE=GATTCCATTCCATTCCATTCACTCATCAAACCAAAAACAATGGCAGCATCAGCATCATCCATGATCTACAAACCAAACCGCCCTAAACACTCCCAACAACCTAACCAAAAACCACCAAACACCAACCAACCTACAAACCATACCAAACCTTATTCGTTACCAAGGTCCTTACCAAACCCTTAANAAGATACAAACAAACTGACAACAAGAAACAAAG
TARGET=2,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02451 Ps_contig_mira-and-tgicl-ass_franssen_21111
SEQUENCE=AAATACGTCGACTCGAAGGACCGGTTTCGTTTGTCAGGCGGGAGTCTGACACGATTTTGTATTCGGATTCATCTCGATCCTATTCTGATCTGTGATCTGTTCTGTTACTGTGTTACTTGATCTATTTTCTACTGTGATTCTGTTACTGATTCTGTTACTATATCATATGATTCTTTTATTGCTCACACCAATAGGTACGTTCATCTTCTTCTTTTAACAAAAGGCGAAGATTGATCGGTTACTATTCGGTTCTGGATCTGTATATCTGTATGTTTTTTTCACTAGATCTGATGTTTTTTTGTTGATTTCTGTTCAGATCATTAACGATCGTGAGACTGGAAGGTCAAGAGGTTTTGGATTCGTGACCTTTGCCACGGAGCAATCCATGAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACG
TARGET=513,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0057
PRIMER_LEFT_PENALTY=0.287765
PRIMER_RIGHT_PENALTY=1.717979
PRIMER_LEFT_SEQUENCE=GTGAGACTGGAAGGTCAAGAGG
PRIMER_RIGHT_SEQUENCE=ATAGCCTCCACCTCCACCATA
PRIMER_LEFT=329,22
PRIMER_RIGHT=546,21
PRIMER_LEFT_TM=60.288
PRIMER_RIGHT_TM=60.718
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=2.0057
PRIMER_LEFT_1_PENALTY=0.287765
PRIMER_RIGHT_1_PENALTY=1.717979
PRIMER_LEFT_1_SEQUENCE=TGAGACTGGAAGGTCAAGAGGT
PRIMER_RIGHT_1_SEQUENCE=ATAGCCTCCACCTCCACCATA
PRIMER_LEFT_1=330,22
PRIMER_RIGHT_1=546,21
PRIMER_LEFT_1_TM=60.288
PRIMER_RIGHT_1_TM=60.718
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=2.0169
PRIMER_LEFT_2_PENALTY=0.298898
PRIMER_RIGHT_2_PENALTY=1.717979
PRIMER_LEFT_2_SEQUENCE=TGGATGGACGTAACATCACAGT
PRIMER_RIGHT_2_SEQUENCE=ATAGCCTCCACCTCCACCATA
PRIMER_LEFT_2=422,22
PRIMER_RIGHT_2=546,21
PRIMER_LEFT_2_TM=60.299
PRIMER_RIGHT_2_TM=60.718
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=2.0221
PRIMER_LEFT_3_PENALTY=0.298898
PRIMER_RIGHT_3_PENALTY=1.723161
PRIMER_LEFT_3_SEQUENCE=TGGATGGACGTAACATCACAGT
PRIMER_RIGHT_3_SEQUENCE=TATCCTCCTCCTCCACCTTCAC
PRIMER_LEFT_3=422,22
PRIMER_RIGHT_3=578,22
PRIMER_LEFT_3_TM=60.299
PRIMER_RIGHT_3_TM=61.723
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=2.0221
PRIMER_LEFT_4_PENALTY=0.298898
PRIMER_RIGHT_4_PENALTY=1.723161
PRIMER_LEFT_4_SEQUENCE=TGGATGGACGTAACATCACAGT
PRIMER_RIGHT_4_SEQUENCE=GTATCCTCCTCCTCCACCTTCA
PRIMER_LEFT_4=422,22
PRIMER_RIGHT_4=579,22
PRIMER_LEFT_4_TM=60.299
PRIMER_RIGHT_4_TM=61.723
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_02452 Ps_contig_mira-and-tgicl-ass_franssen_21111
SEQUENCE=AAATACGTCGACTCGAAGGACCGGTTTCGTTTGTCAGGCGGGAGTCTGACACGATTTTGTATTCGGATTCATCTCGATCCTATTCTGATCTGTGATCTGTTCTGTTACTGTGTTACTTGATCTATTTTCTACTGTGATTCTGTTACTGATTCTGTTACTATATCATATGATTCTTTTATTGCTCACACCAATAGGTACGTTCATCTTCTTCTTTTAACAAAAGGCGAAGATTGATCGGTTACTATTCGGTTCTGGATCTGTATATCTGTATGTTTTTTTCACTAGATCTGATGTTTTTTTGTTGATTTCTGTTCAGATCATTAACGATCGTGAGACTGGAAGGTCAAGAGGTTTTGGATTCGTGACCTTTGCCACGGAGCAATCCATGAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACG
TARGET=566,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02453 Ps_contig_mira-and-tgicl-ass_franssen_21117
SEQUENCE=CGGTTGTCCTAGAAAGGACAAGTGTAGTCAACGCAGAGAGTAACATGAATGATTTGGTAGCAGAGTACCAACAATACCAGGATGCCATTGCCGAGGAGGATGATGAGTATGAAGGAGAAGAAGAAGGTGAGGACCAGTATGTGGAGCATTAGCTGAATGAAACTCTCGGGTAATGCACTAATGGTAAAAATGTCCACTATGCAACTTTGCTGTTATTTGTGTATCTACAGCCGTATATGCCCTTTTTTTATTCTCAATTTTACCCGTTTCTTTACATTTTGCCAATTTCATCTTTTAAGTGTATTATTATTATTATTATCATTTCTGAGTTGTTTGGAAATTGGATTGTGAATTGTCAGACAGGGTTATTCTATACTTCTATTCCTTTAATCATACGTATGTATTTATGAATAAAAAAAAA
TARGET=302,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5136
PRIMER_LEFT_PENALTY=0.009871
PRIMER_RIGHT_PENALTY=0.503696
PRIMER_LEFT_SEQUENCE=TCTACAGCCGTATATGCCCTTT
PRIMER_RIGHT_SEQUENCE=AACCCTGTCTGACAATTCACAA
PRIMER_LEFT=223,22
PRIMER_RIGHT=366,22
PRIMER_LEFT_TM=60.010
PRIMER_RIGHT_TM=59.496
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=0.5314
PRIMER_LEFT_1_PENALTY=0.027712
PRIMER_RIGHT_1_PENALTY=0.503696
PRIMER_LEFT_1_SEQUENCE=GAATGAAACTCTCGGGTAATGC
PRIMER_RIGHT_1_SEQUENCE=AACCCTGTCTGACAATTCACAA
PRIMER_LEFT_1=154,22
PRIMER_RIGHT_1=366,22
PRIMER_LEFT_1_TM=59.972
PRIMER_RIGHT_1_TM=59.496
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.5563
PRIMER_LEFT_2_PENALTY=0.009871
PRIMER_RIGHT_2_PENALTY=0.546410
PRIMER_LEFT_2_SEQUENCE=TCTACAGCCGTATATGCCCTTT
PRIMER_RIGHT_2_SEQUENCE=CCTGTCTGACAATTCACAATCC
PRIMER_LEFT_2=223,22
PRIMER_RIGHT_2=363,22
PRIMER_LEFT_2_TM=60.010
PRIMER_RIGHT_2_TM=59.454
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=141
PRIMER_PAIR_PENALTY_3=0.5563
PRIMER_LEFT_3_PENALTY=0.009871
PRIMER_RIGHT_3_PENALTY=0.546410
PRIMER_LEFT_3_SEQUENCE=TCTACAGCCGTATATGCCCTTT
PRIMER_RIGHT_3_SEQUENCE=CCCTGTCTGACAATTCACAATC
PRIMER_LEFT_3=223,22
PRIMER_RIGHT_3=364,22
PRIMER_LEFT_3_TM=60.010
PRIMER_RIGHT_3_TM=59.454
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=142
PRIMER_PAIR_PENALTY_4=0.5741
PRIMER_LEFT_4_PENALTY=0.027712
PRIMER_RIGHT_4_PENALTY=0.546410
PRIMER_LEFT_4_SEQUENCE=GAATGAAACTCTCGGGTAATGC
PRIMER_RIGHT_4_SEQUENCE=CCTGTCTGACAATTCACAATCC
PRIMER_LEFT_4=154,22
PRIMER_RIGHT_4=363,22
PRIMER_LEFT_4_TM=59.972
PRIMER_RIGHT_4_TM=59.454
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_02454 Ps_contig_mira-and-tgicl-ass_franssen_21129
SEQUENCE=GGAAGAGAACTTAGAAGCACCTAAAGAAACTGAGGAATCGGTTGAAGAAGTGAAGGAAGAGGCGGAAGCAGTAGTGGAGGAGCCAATAGCAACTACTGAAAATGAGTCAGCACCACCACCACCAGCAACTGCAGAGGATGTGATTGAGAACAAAACAGTTGAATCAGTGGAAGCTCCAGCAGCAGAGGAAGTTCCAGTTGAGAACTCCTAGAACGACCTTAAAACCAATGGAAGTGGGTGTGACGTAGGAAACGTAAGGGTAAAAAACGGTTAAGTTTTAAAATTAAGTTGGGTTACTAAGAGTTGGTTAGGGGACGTT
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0550
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.052729
PRIMER_LEFT_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_RIGHT_SEQUENCE=CTCAACTGGAACTTCCTCTGCT
PRIMER_LEFT=41,22
PRIMER_RIGHT=201,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.053
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=0.1622
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.052729
PRIMER_LEFT_1_SEQUENCE=AATCGGTTGAAGAAGTGAAGGA
PRIMER_RIGHT_1_SEQUENCE=CTCAACTGGAACTTCCTCTGCT
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=201,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.053
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.1622
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.052729
PRIMER_LEFT_2_SEQUENCE=GAATCGGTTGAAGAAGTGAAGG
PRIMER_RIGHT_2_SEQUENCE=CTCAACTGGAACTTCCTCTGCT
PRIMER_LEFT_2=34,22
PRIMER_RIGHT_2=201,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.053
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.1622
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.052729
PRIMER_LEFT_3_SEQUENCE=AGGAATCGGTTGAAGAAGTGAA
PRIMER_RIGHT_3_SEQUENCE=CTCAACTGGAACTTCCTCTGCT
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=201,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=60.053
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=0.2191
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.216800
PRIMER_LEFT_4_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_RIGHT_4_SEQUENCE=TCCATTGGTTTTAAGGTCGTTC
PRIMER_LEFT_4=41,22
PRIMER_RIGHT_4=231,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=60.217
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_02455 Ps_contig_mira-and-tgicl-ass_franssen_21131
SEQUENCE=TTTGTTCTCAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTTGCTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTTCACTTCTTCAACCGATTCCTCAGTTTCTTTAGGTGCTTCTAAGTTCTCTTCCTTTGCTTCCTCTGTTACCACTTCCTTGTTCTCAACTTCAAGTGGATCTTGGGTTTCGGANCATCTACAGCTTCTCCTGCTGCAGTTGTTACTGTTTGGTTTTTCGKNTTCTNTGTTGGTACTTCGGNGCAGGAACTTCGGATTCTGGTTGTGCCCGNAGG
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02456 Ps_contig_mira-and-tgicl-ass_franssen_21136
SEQUENCE=AAGCAGTTGGTTATTCAACGCAGAGTASGTCGGGGATATCAAACACACACATAACATATTAAGTGAAGTGAGTCCTATTAAGTTTACACATTCATCACTAATTAAGTTATGGAGAAGAAATCACTAGCTGCCTTGTCCTTCCTCTTCCTTTTTCTCTTTGTTGCACAAGAAATTGTGGTGACAGAGGCAAACACCTGTGAGCATTTGGCAGATACATACAGGGGAGTATGCTTCACGGATGCTAGCTGTGATGATCACTGCAAGAACAAAGCGCACTTAATCAGTGGCACTTGCCATAACTTTAAATGTTTCTGCACTCAAAACTGTTAAACTTGGATGGATGGATTGATTCTCCAACACAAAGACAAAGACGGATAATATAAATAAATAAATAAAATGCATGCTGTAGTAGCTTGCACCGCATCTATCTATGTATATGAGTATTGCGTCTTCTTTGAATCATATATATGTGTAATAAATATCTGCATAACGTATCAGTTGTGCACTTTATCATATGGAATAAAAATATTATTAATCAATAATTTTAGTTTTAAAGTTTTAAAAAGTT
TARGET=334,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02457 Ps_contig_mira-and-tgicl-ass_franssen_21136
SEQUENCE=AAGCAGTTGGTTATTCAACGCAGAGTASGTCGGGGATATCAAACACACACATAACATATTAAGTGAAGTGAGTCCTATTAAGTTTACACATTCATCACTAATTAAGTTATGGAGAAGAAATCACTAGCTGCCTTGTCCTTCCTCTTCCTTTTTCTCTTTGTTGCACAAGAAATTGTGGTGACAGAGGCAAACACCTGTGAGCATTTGGCAGATACATACAGGGGAGTATGCTTCACGGATGCTAGCTGTGATGATCACTGCAAGAACAAAGCGCACTTAATCAGTGGCACTTGCCATAACTTTAAATGTTTCTGCACTCAAAACTGTTAAACTTGGATGGATGGATTGATTCTCCAACACAAAGACAAAGACGGATAATATAAATAAATAAATAAAATGCATGCTGTAGTAGCTTGCACCGCATCTATCTATGTATATGAGTATTGCGTCTTCTTTGAATCATATATATGTGTAATAAATATCTGCATAACGTATCAGTTGTGCACTTTATCATATGGAATAAAAATATTATTAATCAATAATTTTAGTTTTAAAGTTTTAAAAAGTT
TARGET=380,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02458 Ps_contig_mira-and-tgicl-ass_franssen_21159
SEQUENCE=GTCGGGGGAGAAGGAGAAAGAGAAAGGAAAAGATAGGTATGAAGAACCTGAGCTTGAACCTGATTCCGTGTCTACCGGACGATGTTGCTGTGAACTGTCTCGTACGTGTTCCACGCTCTCAACACACAACCCTCTCTCTTGTTTCGAAATCATTCCGTTCTCTCCTCTCTTCTCCACTCTTCTTCACCGCTCGCTCTCTCCTTCATTCCACCCAACACATCCTCTACCTCTCTATTCGTACACGCGCCTCCACTCTCCAATGGTTCACGCTCAACAACAACAACCACCTCATTCCTCTTCCTCCTCTCCCTTCCCCTGCGATCGGCTCTGCCTACGCAGTCATTGGACATGCAATCTATGTCATCGGCGGTTCAATCAATGATATCCCCTCCCGTCATGTATGGATCCTTGATTGCCGATTCCACCGTTGGCATCCTGGTCCTTCCATGCGCGTCTCACGTGAGTTTGCCGCTGCTGGCGTCGTTGACGGCAAGATTTACGTCATCGGCGGTTGCGTTCCTGATAATTGGTCTAGGTCAGCTAATTGGGCGGAGGTATTTAATCCTGCTACCAACATCTGGGAAGGCATTCCCAGCCCGCCGGAGATTCGCGAGAAGTGGATGCACGCCAGCGCAGTTGTAGACTGTAAGATTTACGCCATGGCTGACCGCGGTGGTGTTTCCCTTGATCCTTGTAGCAGCGCGTGGGAGAGTGTGGGGAGTGAACTTGACCTAGGGTGGCGTGGGAGAGCTTGCGTTGTTAATGGGATTCTGTATTGCTACAATTACTTGGGGAAAATCAAAGGTTTCGACGTGAAAGAAAGGTTGTGGAAGGAGTTGAACGGTGTGGAAAAGGGTTTGCCGAGGTTTCTTTGTGGGGCAACAATGGCTGATGTTGGTGGAAAATTGGTTGTGGTTTGGGAGTGCCAAGGGAATGCGACTGAAAAGGGGAAAGAAATGGAGATTTGGTGCGCTGAGATTGATGTGAACAAGAACAAAGATGGGGAATTGTGGGGTGAAGTTTGTTGGTTGAACAATGTTCTCTCTGTTCCAAAAGGATCCTCCATTGTTCATTGTTCTTCTGTTGCCTTGTGATTATTGTGCCTCACCAGGTCACCAGTTTCTACCATTACATGTCCTGCAGGATCAAGATCCTCAAACACCTGGTGGTAACACTAGATTTCTCCTTCTTGGTCTTGCAAATTCTTTCTAGTTTGATCGGATGTCATTTATTGCTCCCCTGGTGTATGGATAAACAGGAGCTGTAATGTTTGTATGTACAGTTTTGCACATGCATAGTTATGTGTTCCCGATATTTGCATTGTGTATCCTTGCCAATCTGGCAATCCATATGTTGCACATAGTTAAGTAATGGTGAGGTTTAA
TARGET=272,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0211
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.015502
PRIMER_LEFT_SEQUENCE=CCCAACACATCCTCTACCTCTC
PRIMER_RIGHT_SEQUENCE=GGAGGGGATATCATTGATTGAA
PRIMER_LEFT=210,22
PRIMER_RIGHT=391,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=59.984
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=0.0641
PRIMER_LEFT_1_PENALTY=0.048570
PRIMER_RIGHT_1_PENALTY=0.015502
PRIMER_LEFT_1_SEQUENCE=GTTTCGAAATCATTCCGTTCTC
PRIMER_RIGHT_1_SEQUENCE=GGAGGGGATATCATTGATTGAA
PRIMER_LEFT_1=140,22
PRIMER_RIGHT_1=391,22
PRIMER_LEFT_1_TM=59.951
PRIMER_RIGHT_1_TM=59.984
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.2125
PRIMER_LEFT_2_PENALTY=0.197024
PRIMER_RIGHT_2_PENALTY=0.015502
PRIMER_LEFT_2_SEQUENCE=CAACACACAACCCTCTCTCTTG
PRIMER_RIGHT_2_SEQUENCE=GGAGGGGATATCATTGATTGAA
PRIMER_LEFT_2=119,22
PRIMER_RIGHT_2=391,22
PRIMER_LEFT_2_TM=59.803
PRIMER_RIGHT_2_TM=59.984
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.3933
PRIMER_LEFT_3_PENALTY=0.377848
PRIMER_RIGHT_3_PENALTY=0.015502
PRIMER_LEFT_3_SEQUENCE=CGTTCTCTCCTCTCTTCTCCAC
PRIMER_RIGHT_3_SEQUENCE=GGAGGGGATATCATTGATTGAA
PRIMER_LEFT_3=155,22
PRIMER_RIGHT_3=391,22
PRIMER_LEFT_3_TM=59.622
PRIMER_RIGHT_3_TM=59.984
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=0.4429
PRIMER_LEFT_4_PENALTY=0.160828
PRIMER_RIGHT_4_PENALTY=0.282090
PRIMER_LEFT_4_SEQUENCE=ATGTTGCTGTGAACTGTCTCGT
PRIMER_RIGHT_4_SEQUENCE=AGAGGAGGAAGAGGAATGAGGT
PRIMER_LEFT_4=81,22
PRIMER_RIGHT_4=306,22
PRIMER_LEFT_4_TM=59.839
PRIMER_RIGHT_4_TM=59.718
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_02459 Ps_contig_mira-and-tgicl-ass_franssen_21172
SEQUENCE=TCGGGGATATCAAACACACAACATATTAAGTGAAGTGAATCATATATATATTAAATCTATATTCATCATCACTAAAGTTATGGAGAAGAAATCACTAGCTGCCTTGTCCTTCCTCCTCCTCCTCGTTCTCTTTGTTGCACAAGAAATTGTGGTGACAGAGGCAAACACTTGTGAGCATTTGGCTGATACATACAGGGGAGTATGCTTCACGAATGCTAGCTGTGATGATCACTGCAAGAACAAAGCGCACTTAATCAGTGGCACGTGCCATGACTGGAAATGTTTCTGCACTCAAAACTGTTAAAGACGTTATAAGTATAATAATATAAATAAATAAAATGCATGCAGTTATAGCAACTACCGCTGTATCTGTGTATGTATTTGAATAAGTTATGTGTGTACTCATCGTGTATAACCTACTAGTTATGCACTTTTATCTTATGGAATAAAACTTTAATACAATAAAAA
TARGET=42,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1499
PRIMER_LEFT_PENALTY=1.103105
PRIMER_RIGHT_PENALTY=0.046801
PRIMER_LEFT_SEQUENCE=CGGGGATATCAAACACACAAC
PRIMER_RIGHT_SEQUENCE=CTCACAAGTGTTTGCCTCTGTC
PRIMER_LEFT=1,21
PRIMER_RIGHT=174,22
PRIMER_LEFT_TM=60.103
PRIMER_RIGHT_TM=59.953
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=1.1898
PRIMER_LEFT_1_PENALTY=1.103105
PRIMER_RIGHT_1_PENALTY=0.086698
PRIMER_LEFT_1_SEQUENCE=CGGGGATATCAAACACACAAC
PRIMER_RIGHT_1_SEQUENCE=GAAGGACAAGGCAGCTAGTGAT
PRIMER_LEFT_1=1,21
PRIMER_RIGHT_1=111,22
PRIMER_LEFT_1_TM=60.103
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=1.1916
PRIMER_LEFT_2_PENALTY=1.103105
PRIMER_RIGHT_2_PENALTY=0.088479
PRIMER_LEFT_2_SEQUENCE=CGGGGATATCAAACACACAAC
PRIMER_RIGHT_2_SEQUENCE=CTTGTGCAACAAAGAGAACGAG
PRIMER_LEFT_2=1,21
PRIMER_RIGHT_2=142,22
PRIMER_LEFT_2_TM=60.103
PRIMER_RIGHT_2_TM=60.088
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=1.1985
PRIMER_LEFT_3_PENALTY=1.103105
PRIMER_RIGHT_3_PENALTY=0.095445
PRIMER_LEFT_3_SEQUENCE=CGGGGATATCAAACACACAAC
PRIMER_RIGHT_3_SEQUENCE=CCCCTGTATGTATCAGCCAAAT
PRIMER_LEFT_3=1,21
PRIMER_RIGHT_3=197,22
PRIMER_LEFT_3_TM=60.103
PRIMER_RIGHT_3_TM=60.095
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=1.3230
PRIMER_LEFT_4_PENALTY=1.103105
PRIMER_RIGHT_4_PENALTY=0.219884
PRIMER_LEFT_4_SEQUENCE=CGGGGATATCAAACACACAAC
PRIMER_RIGHT_4_SEQUENCE=AGTGCAGAAACATTTCCAGTCA
PRIMER_LEFT_4=1,21
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=59.780
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_02460 Ps_contig_mira-and-tgicl-ass_franssen_21172
SEQUENCE=TCGGGGATATCAAACACACAACATATTAAGTGAAGTGAATCATATATATATTAAATCTATATTCATCATCACTAAAGTTATGGAGAAGAAATCACTAGCTGCCTTGTCCTTCCTCCTCCTCCTCGTTCTCTTTGTTGCACAAGAAATTGTGGTGACAGAGGCAAACACTTGTGAGCATTTGGCTGATACATACAGGGGAGTATGCTTCACGAATGCTAGCTGTGATGATCACTGCAAGAACAAAGCGCACTTAATCAGTGGCACGTGCCATGACTGGAAATGTTTCTGCACTCAAAACTGTTAAAGACGTTATAAGTATAATAATATAAATAAATAAAATGCATGCAGTTATAGCAACTACCGCTGTATCTGTGTATGTATTTGAATAAGTTATGTGTGTACTCATCGTGTATAACCTACTAGTTATGCACTTTTATCTTATGGAATAAAACTTTAATACAATAAAAA
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3650
PRIMER_LEFT_PENALTY=0.269585
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=GAAATCACTAGCTGCCTTGTCC
PRIMER_RIGHT_SEQUENCE=CCCCTGTATGTATCAGCCAAAT
PRIMER_LEFT=87,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=60.270
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=111
PRIMER_PAIR_PENALTY_1=0.4895
PRIMER_LEFT_1_PENALTY=0.269585
PRIMER_RIGHT_1_PENALTY=0.219884
PRIMER_LEFT_1_SEQUENCE=GAAATCACTAGCTGCCTTGTCC
PRIMER_RIGHT_1_SEQUENCE=TGAGTGCAGAAACATTTCCAGT
PRIMER_LEFT_1=87,22
PRIMER_RIGHT_1=293,22
PRIMER_LEFT_1_TM=60.270
PRIMER_RIGHT_1_TM=59.780
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.4895
PRIMER_LEFT_2_PENALTY=0.269585
PRIMER_RIGHT_2_PENALTY=0.219884
PRIMER_LEFT_2_SEQUENCE=GAAATCACTAGCTGCCTTGTCC
PRIMER_RIGHT_2_SEQUENCE=AGTGCAGAAACATTTCCAGTCA
PRIMER_LEFT_2=87,22
PRIMER_RIGHT_2=291,22
PRIMER_LEFT_2_TM=60.270
PRIMER_RIGHT_2_TM=59.780
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.4950
PRIMER_LEFT_3_PENALTY=0.269585
PRIMER_RIGHT_3_PENALTY=0.225403
PRIMER_LEFT_3_SEQUENCE=GAAATCACTAGCTGCCTTGTCC
PRIMER_RIGHT_3_SEQUENCE=GTATCAGCCAAATGCTCACAAG
PRIMER_LEFT_3=87,22
PRIMER_RIGHT_3=188,22
PRIMER_LEFT_3_TM=60.270
PRIMER_RIGHT_3_TM=59.775
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=102
PRIMER_PAIR_PENALTY_4=0.4950
PRIMER_LEFT_4_PENALTY=0.269585
PRIMER_RIGHT_4_PENALTY=0.225403
PRIMER_LEFT_4_SEQUENCE=GAAATCACTAGCTGCCTTGTCC
PRIMER_RIGHT_4_SEQUENCE=TATCAGCCAAATGCTCACAAGT
PRIMER_LEFT_4=87,22
PRIMER_RIGHT_4=187,22
PRIMER_LEFT_4_TM=60.270
PRIMER_RIGHT_4_TM=59.775
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_02461 Ps_contig_mira-and-tgicl-ass_franssen_21172
SEQUENCE=TCGGGGATATCAAACACACAACATATTAAGTGAAGTGAATCATATATATATTAAATCTATATTCATCATCACTAAAGTTATGGAGAAGAAATCACTAGCTGCCTTGTCCTTCCTCCTCCTCCTCGTTCTCTTTGTTGCACAAGAAATTGTGGTGACAGAGGCAAACACTTGTGAGCATTTGGCTGATACATACAGGGGAGTATGCTTCACGAATGCTAGCTGTGATGATCACTGCAAGAACAAAGCGCACTTAATCAGTGGCACGTGCCATGACTGGAAATGTTTCTGCACTCAAAACTGTTAAAGACGTTATAAGTATAATAATATAAATAAATAAAATGCATGCAGTTATAGCAACTACCGCTGTATCTGTGTATGTATTTGAATAAGTTATGTGTGTACTCATCGTGTATAACCTACTAGTTATGCACTTTTATCTTATGGAATAAAACTTTAATACAATAAAAA
TARGET=326,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5410
PRIMER_LEFT_PENALTY=0.046801
PRIMER_RIGHT_PENALTY=1.494171
PRIMER_LEFT_SEQUENCE=GACAGAGGCAAACACTTGTGAG
PRIMER_RIGHT_SEQUENCE=GCGGTAGTTGCTATAACTGCAT
PRIMER_LEFT=153,22
PRIMER_RIGHT=363,22
PRIMER_LEFT_TM=59.953
PRIMER_RIGHT_TM=58.506
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=1.5474
PRIMER_LEFT_1_PENALTY=0.046801
PRIMER_RIGHT_1_PENALTY=1.500551
PRIMER_LEFT_1_SEQUENCE=GACAGAGGCAAACACTTGTGAG
PRIMER_RIGHT_1_SEQUENCE=CACAGATACAGCGGTAGTTGCTA
PRIMER_LEFT_1=153,22
PRIMER_RIGHT_1=373,23
PRIMER_LEFT_1_TM=59.953
PRIMER_RIGHT_1_TM=59.499
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=1.5809
PRIMER_LEFT_2_PENALTY=0.086698
PRIMER_RIGHT_2_PENALTY=1.494171
PRIMER_LEFT_2_SEQUENCE=ATCACTAGCTGCCTTGTCCTTC
PRIMER_RIGHT_2_SEQUENCE=GCGGTAGTTGCTATAACTGCAT
PRIMER_LEFT_2=90,22
PRIMER_RIGHT_2=363,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=58.506
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=1.5872
PRIMER_LEFT_3_PENALTY=0.086698
PRIMER_RIGHT_3_PENALTY=1.500551
PRIMER_LEFT_3_SEQUENCE=ATCACTAGCTGCCTTGTCCTTC
PRIMER_RIGHT_3_SEQUENCE=CACAGATACAGCGGTAGTTGCTA
PRIMER_LEFT_3=90,22
PRIMER_RIGHT_3=373,23
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=59.499
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=1.5896
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=1.494171
PRIMER_LEFT_4_SEQUENCE=ATTTGGCTGATACATACAGGGG
PRIMER_RIGHT_4_SEQUENCE=GCGGTAGTTGCTATAACTGCAT
PRIMER_LEFT_4=176,22
PRIMER_RIGHT_4=363,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=58.506
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_02462 Ps_contig_mira-and-tgicl-ass_franssen_21174
SEQUENCE=AGTGGTAAGAGTGAAGTGGGGGAGAGGCACACGTTTTGAGCAGGCAAATCCATGTGCTCACATGGATAGAGAGAAATAGTAGAACGAAGAAGAAGAAGACGGTGATGAATCAATAAAAATCTCAAACCCTAAAAGTTCTGATTTTTTTGATTTCGTTTTTGATCGATTCATATATTCATAGATTACAAGATGAAGGAAAGTAGTGATGGATTTGTTAGAGCGGATCAGATTGATCTAGAAAAGCATCGATGAACAGCTTGAGAGACACCTCAACAAAGTGTTGACTATTGACAAGAAAAAAGCGCGTTGAAGAACAAGATTCTACCGCTTCTAATGTTTTTGATCATGCTCAGCTACACCTATTCTTGGTTGTGGCTATTGTGGAAAACCACATAAAAAACACAAACCAGGTAAAAAACCAATCGTCACTCCACCAACCGTTAAACCAA
TARGET=86,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8132
PRIMER_LEFT_PENALTY=0.749986
PRIMER_RIGHT_PENALTY=0.063211
PRIMER_LEFT_SEQUENCE=GTAAGAGTGAAGTGGGGGAGAG
PRIMER_RIGHT_SEQUENCE=GATCCGCTCTAACAAATCCATC
PRIMER_LEFT=4,22
PRIMER_RIGHT=225,22
PRIMER_LEFT_TM=59.250
PRIMER_RIGHT_TM=59.937
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.9049
PRIMER_LEFT_1_PENALTY=0.749986
PRIMER_RIGHT_1_PENALTY=0.154878
PRIMER_LEFT_1_SEQUENCE=GTAAGAGTGAAGTGGGGGAGAG
PRIMER_RIGHT_1_SEQUENCE=TCAATCTGATCCGCTCTAACAA
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=59.250
PRIMER_RIGHT_1_TM=59.845
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=229
PRIMER_PAIR_PENALTY_2=1.0781
PRIMER_LEFT_2_PENALTY=1.019966
PRIMER_RIGHT_2_PENALTY=0.058146
PRIMER_LEFT_2_SEQUENCE=GGTAAGAGTGAAGTGGGGGAG
PRIMER_RIGHT_2_SEQUENCE=GTGTCTCTCAAGCTGTTCATCG
PRIMER_LEFT_2=3,21
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=60.058
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=1.0832
PRIMER_LEFT_3_PENALTY=1.019966
PRIMER_RIGHT_3_PENALTY=0.063211
PRIMER_LEFT_3_SEQUENCE=GGTAAGAGTGAAGTGGGGGAG
PRIMER_RIGHT_3_SEQUENCE=GATCCGCTCTAACAAATCCATC
PRIMER_LEFT_3=3,21
PRIMER_RIGHT_3=225,22
PRIMER_LEFT_3_TM=59.980
PRIMER_RIGHT_3_TM=59.937
PRIMER_LEFT_3_GC_PERCENT=57.143
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=1.1506
PRIMER_LEFT_4_PENALTY=0.749986
PRIMER_RIGHT_4_PENALTY=0.400659
PRIMER_LEFT_4_SEQUENCE=GTAAGAGTGAAGTGGGGGAGAG
PRIMER_RIGHT_4_SEQUENCE=AGCTGTTCATCGATGCTTTTCT
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=59.250
PRIMER_RIGHT_4_TM=60.401
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_02463 Ps_contig_mira-and-tgicl-ass_franssen_21175
SEQUENCE=AGTAACTAACGGGGACTACGAAATGAGTCACTATTATAAATTCATTCATTCCCTTTGGATATACGATCTTGCTTCAAAACACCACACATTTCTCTATCTCAGCAAAACAACATAGCCATGGCCACATCAATCTCTGCTGTTAATCCTATCAGCAAAACTCCATTGAGTTTGAATAATTCTCGAGCAGTAGCTTCAGCTTCAAGTTCATCTTTCTTTGGAAGCAGTTTAAAGAAGGTTATCTCAAAGGTTTCAAACACCAGGGTTTCTTCCAATGGAAGGTTCAAAGTTGTTGCTGCTGAGATTGATGAGAGCAAGCAAACTGATAAGGATAGATGGAGAGGTTTGGCTTATGATGTTTCGGATGATCAACAAGATATCACGAGAGGGAAGGGTTTGGTTGACTCAGTTTTTCAAGCTCCGGATAATTCTGGAACTCATTATGCAGTCATGAGTTCTTATGAGTACATTAGTACCGGACTTAAACAGTGAGTCATAGATAGATATTCTTTCAACTGTGTCGGTGTCGGTGT
TARGET=40,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0740
PRIMER_LEFT_PENALTY=0.014720
PRIMER_RIGHT_PENALTY=0.059316
PRIMER_LEFT_SEQUENCE=CTAACGGGGACTACGAAATGAG
PRIMER_RIGHT_SEQUENCE=ATCTCAGCAGCAACAACTTTGA
PRIMER_LEFT=5,22
PRIMER_RIGHT=301,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=60.059
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.0740
PRIMER_LEFT_1_PENALTY=0.014720
PRIMER_RIGHT_1_PENALTY=0.059316
PRIMER_LEFT_1_SEQUENCE=CTAACGGGGACTACGAAATGAG
PRIMER_RIGHT_1_SEQUENCE=TCAATCTCAGCAGCAACAACTT
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=304,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=60.059
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.1104
PRIMER_LEFT_2_PENALTY=0.014720
PRIMER_RIGHT_2_PENALTY=0.095684
PRIMER_LEFT_2_SEQUENCE=CTAACGGGGACTACGAAATGAG
PRIMER_RIGHT_2_SEQUENCE=CTCAGCAGCAACAACTTTGAAC
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=299,22
PRIMER_LEFT_2_TM=60.015
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.1671
PRIMER_LEFT_3_PENALTY=0.107763
PRIMER_RIGHT_3_PENALTY=0.059316
PRIMER_LEFT_3_SEQUENCE=ACTAACGGGGACTACGAAATGA
PRIMER_RIGHT_3_SEQUENCE=ATCTCAGCAGCAACAACTTTGA
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=301,22
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=60.059
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.1671
PRIMER_LEFT_4_PENALTY=0.107763
PRIMER_RIGHT_4_PENALTY=0.059316
PRIMER_LEFT_4_SEQUENCE=TAACGGGGACTACGAAATGAGT
PRIMER_RIGHT_4_SEQUENCE=ATCTCAGCAGCAACAACTTTGA
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=60.059
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_02464 Ps_contig_mira-and-tgicl-ass_franssen_21201
SEQUENCE=GACCCAGAGGACAAAGTTTAGTGGGAGATAGAGATAGAGAGAGAGAGTTGCATAATAATAAGAGCTTCAGTTTCTCTCTCTACAAACTCAACCGTGTCTTTTACTCGTTAGATTTTTTAGTTTTCATGAAGATTCACTCTCTCTTCCCCAACCTTGACTGGCAAGCCAGTCAAGCTGAA
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7037
PRIMER_LEFT_PENALTY=0.026443
PRIMER_RIGHT_PENALTY=0.677241
PRIMER_LEFT_SEQUENCE=CCAGAGGACAAAGTTTAGTGGG
PRIMER_RIGHT_SEQUENCE=TGGGGAAGAGAGAGTGAATCTT
PRIMER_LEFT=3,22
PRIMER_RIGHT=149,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=59.323
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=147
PRIMER_PAIR_PENALTY_1=0.7037
PRIMER_LEFT_1_PENALTY=0.026443
PRIMER_RIGHT_1_PENALTY=0.677241
PRIMER_LEFT_1_SEQUENCE=CCCAGAGGACAAAGTTTAGTGG
PRIMER_RIGHT_1_SEQUENCE=TGGGGAAGAGAGAGTGAATCTT
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=149,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=59.323
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=148
PRIMER_PAIR_PENALTY_2=0.8395
PRIMER_LEFT_2_PENALTY=0.026443
PRIMER_RIGHT_2_PENALTY=0.813080
PRIMER_LEFT_2_SEQUENCE=CCAGAGGACAAAGTTTAGTGGG
PRIMER_RIGHT_2_SEQUENCE=GTTGGGGAAGAGAGAGTGAATC
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=151,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=59.187
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.8395
PRIMER_LEFT_3_PENALTY=0.026443
PRIMER_RIGHT_3_PENALTY=0.813080
PRIMER_LEFT_3_SEQUENCE=CCCAGAGGACAAAGTTTAGTGG
PRIMER_RIGHT_3_SEQUENCE=GTTGGGGAAGAGAGAGTGAATC
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=151,22
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=59.187
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=150
PRIMER_PAIR_PENALTY_4=0.8718
PRIMER_LEFT_4_PENALTY=0.026443
PRIMER_RIGHT_4_PENALTY=0.845332
PRIMER_LEFT_4_SEQUENCE=CCAGAGGACAAAGTTTAGTGGG
PRIMER_RIGHT_4_SEQUENCE=GGTTGGGGAAGAGAGAGTGAAT
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=152,22
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=60.845
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_02465 Ps_contig_mira-and-tgicl-ass_franssen_21237
SEQUENCE=GTGGTGGTGGTGGTCATCATSGTGGRSTWMTWTGGTTGGTGCTTCCGACAATGGTAACTGATGATACCATGATAAATGATGCACATTCAAAGTAATAAGTTTCATGAATGAATAATGTTTGGTGTTAAAATAAAAGAAATACCAATCTGTTGTGGTTATGTATCATCTATGTATATGTGAAGACGTTGTTTGATTTCGAAGTAAARGTGATGAATTGGTTGTTATCTATTTTGTTATAATATCAAAATCTCATTTCCCG
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02466 Ps_contig_mira-and-tgicl-ass_franssen_21261
SEQUENCE=ACGSCMACAAATTCACGTTATCACATTCCTGAATTCACCGCTCAAAATTTCATCTCATCTGAGCGCTTCCTTCCGCCGCGCCGGAAATCAACACCGGCGGCGGCGGAAACCTAAACTACCGAAATCTTGCACCAGATCCTCCGTCTCCATAACCTCGACCTGAATTCCTCACTTAATTCAACCGAATCTCACGCGATCGACCTAACCCGNGCATTCCACTTGAAGTTGAGAACAAGGAAGTGGTAACAGAGGAAGCAAAGGAAGAGAA
TARGET=95,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02467 Ps_contig_mira-and-tgicl-ass_franssen_21264
SEQUENCE=TAAAAGAAGACATAAACAACCCCAAACTTTTGGGTAAAGTTTTACCCTTGACTAATGCAGAAGTAAGCAAATCCATGCAATGTCCTTGCTAACCCAGATAGGCAGGCACACTTTTTGAGGAATTGCAACTGAATCTTTGTTTTTTCGAAACGGTGTATAATGTAGAACGCAAATGTGTTTTGATCATCATCAAGCCTTACCAAACCCCAGAGTAAGGTCCAATTCATCCATTCCTACCTCGTGAATTCTCTCCCCTTCCCACGGCTTCACGACTCTTCCATATTCCTAGGGAGCTTTATCACTTTCTAGTTTCATCTTTCTTTGGATTGAAGGGAGCTCTACACCTTTATTTCTTATATCCATTTTTTTTAATTTAAGAAAGTAGTTTCAAATTTTATCCAGTATTTTAATTTAGTTTCTATCGAATAATTTACTGTTTGTTGCATAGTATATTGTTCATATAATTTTGATCTACACTAGATCTTTGAGATCTTTGTTGGTGAGGTATTTTTAATGTAATATTTCTAAAGTTATGCATTATCTATTAATTTACTTGGCCATAAGCATAGTTTTCATCTAAAATTGTTTTTCATTCTAGATCTGGGAATTCTTCTAAAGATGAAGTGATATTTATCACTTTTAACTGAGTTTTCTTTACCATTTTGTTTATTCATTTGCCGTTGGATATTATTTTTGCTAATATTTGTTGTAGTTTCAGATGGATCTGTACTTGAAGATGTGATTTATAAGTGGTCTTGGCAATGATTGTGATAATTTAACTTGTAAAAATTCATAATGTAATGTAACGGCTTACATATTTATTATTATTATTATGTTGATATAACTTTATGAATAACTTTCGAGGTTGATWAWYCACGGTTTTCGGTATTGT
TARGET=819,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02468 Ps_contig_mira-and-tgicl-ass_franssen_21269
SEQUENCE=CGGGCGAAAGAGACGGCGTGACGACTTTCTCCGATCACCTGAAGTAACTGCTCCTCCACGTCGGAGACCATTCGAATAGGATTGGAGTCATGGAAGCTGAATCCGGCGGCGGCGGAGGCGGTGGTGTGGGACATATGCAGTCTGGCCTTCCAGATCCATCTTCTTTGCCTTTGTATAACCTGCCACCAAATCTTCTCCCTAATTGGTGGCCATGTGGGACATGAAGCTTTTCCATGGCCTCATGGAATTAGGCCACCATACTGAAACAAATGATATGAATCTGATGTCATGAAATTGGATATTTATATAGTTATATTATATTGTAGTGTTTGTTTGTGATAAAATGTGATGAGAAGTGAGAAGAGGAAGCAATGTTATATTATAATTTATCCATCACTCTCTCTATNTA
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0552
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.023406
PRIMER_LEFT_SEQUENCE=ATTGGAGTCATGGAAGCTGAAT
PRIMER_RIGHT_SEQUENCE=GGCAGGTTATACAAAGGCAAAG
PRIMER_LEFT=80,22
PRIMER_RIGHT=183,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.023
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=0.1272
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=ATTGGAGTCATGGAAGCTGAAT
PRIMER_RIGHT_1_SEQUENCE=CAGTATGGTGGCCTAATTCCAT
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=262,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.1646
PRIMER_LEFT_2_PENALTY=0.031769
PRIMER_RIGHT_2_PENALTY=0.132857
PRIMER_LEFT_2_SEQUENCE=ATTGGAGTCATGGAAGCTGAAT
PRIMER_RIGHT_2_SEQUENCE=TCATTTGTTTCAGTATGGTGGC
PRIMER_LEFT_2=80,22
PRIMER_RIGHT_2=272,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=193
PRIMER_PAIR_PENALTY_3=0.2247
PRIMER_LEFT_3_PENALTY=0.201288
PRIMER_RIGHT_3_PENALTY=0.023406
PRIMER_LEFT_3_SEQUENCE=GACCATTCGAATAGGATTGGAG
PRIMER_RIGHT_3_SEQUENCE=GGCAGGTTATACAAAGGCAAAG
PRIMER_LEFT_3=65,22
PRIMER_RIGHT_3=183,22
PRIMER_LEFT_3_TM=59.799
PRIMER_RIGHT_3_TM=60.023
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=0.2247
PRIMER_LEFT_4_PENALTY=0.201288
PRIMER_RIGHT_4_PENALTY=0.023406
PRIMER_LEFT_4_SEQUENCE=CCATTCGAATAGGATTGGAGTC
PRIMER_RIGHT_4_SEQUENCE=GGCAGGTTATACAAAGGCAAAG
PRIMER_LEFT_4=67,22
PRIMER_RIGHT_4=183,22
PRIMER_LEFT_4_TM=59.799
PRIMER_RIGHT_4_TM=60.023
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=117
=
PRIMER_SEQUENCE_ID=SSR_02469 Ps_contig_mira-and-tgicl-ass_franssen_21286
SEQUENCE=TTAATTTTAGGTTTTTGGGTTCCGTTTTAATTTTAATTTAATTACTTTTGGGTCCACCTTCTTAGCAATGGTTATTGATCCTAGTTTCTTTTGGTTTAATTAAGATTACTATGTTGTTCGTTTATTGTTGTTTTAATTTTGTTCTTTTTCTTTGTTCCCTTTTATTGTTTTATTTAAGTATGATGATGATGATGATGATGATGTATAGTTTATTTGTATGAGATATTCAACAAGTTAAGTTCCTTGGATTAGAGCATTTTGATTTAGAA
TARGET=180,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02470 Ps_contig_mira-and-tgicl-ass_franssen_21341
SEQUENCE=AAAATAACTTAAAGTAAAACAAATAGGAACCAAACCATGCATTCAACAACACTGCAGGAACTCAGAATTTGGAAAGGCCAAGATCAACACTGGAGCCAGGATTGGAAATTAAATGGGGCTTCCGTGACGTACACATATACGTATAATATGTATATGTGTGTGTTTATGGGTAATAAAAAGTAAATAAATAAAGTGTTTTAATTATGTTTATGTGTATCTTGTGAACTGTATTCCTGTGAACTAAATATGAAATAATAATTAAACTGGTATGTTTAACT
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.4308
PRIMER_LEFT_PENALTY=0.046801
PRIMER_RIGHT_PENALTY=3.383967
PRIMER_LEFT_SEQUENCE=ACAACACTGCAGGAACTCAGAA
PRIMER_RIGHT_SEQUENCE=GTTCACAGGAATACAGTTCACAAGA
PRIMER_LEFT=45,22
PRIMER_RIGHT=240,25
PRIMER_LEFT_TM=59.953
PRIMER_RIGHT_TM=59.616
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=3.4308
PRIMER_LEFT_1_PENALTY=0.046801
PRIMER_RIGHT_1_PENALTY=3.383967
PRIMER_LEFT_1_SEQUENCE=AACAACACTGCAGGAACTCAGA
PRIMER_RIGHT_1_SEQUENCE=GTTCACAGGAATACAGTTCACAAGA
PRIMER_LEFT_1=44,22
PRIMER_RIGHT_1=240,25
PRIMER_LEFT_1_TM=59.953
PRIMER_RIGHT_1_TM=59.616
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=3.4658
PRIMER_LEFT_2_PENALTY=0.081801
PRIMER_RIGHT_2_PENALTY=3.383967
PRIMER_LEFT_2_SEQUENCE=ATAGGAACCAAACCATGCATTC
PRIMER_RIGHT_2_SEQUENCE=GTTCACAGGAATACAGTTCACAAGA
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=240,25
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=59.616
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=3.4997
PRIMER_LEFT_3_PENALTY=0.115769
PRIMER_RIGHT_3_PENALTY=3.383967
PRIMER_LEFT_3_SEQUENCE=ACAAATAGGAACCAAACCATGC
PRIMER_RIGHT_3_SEQUENCE=GTTCACAGGAATACAGTTCACAAGA
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=240,25
PRIMER_LEFT_3_TM=60.116
PRIMER_RIGHT_3_TM=59.616
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=3.7183
PRIMER_LEFT_4_PENALTY=0.334292
PRIMER_RIGHT_4_PENALTY=3.383967
PRIMER_LEFT_4_SEQUENCE=TTCAACAACACTGCAGGAACTC
PRIMER_RIGHT_4_SEQUENCE=GTTCACAGGAATACAGTTCACAAGA
PRIMER_LEFT_4=41,22
PRIMER_RIGHT_4=240,25
PRIMER_LEFT_4_TM=60.334
PRIMER_RIGHT_4_TM=59.616
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_02471 Ps_contig_mira-and-tgicl-ass_franssen_21349
SEQUENCE=CGTTTTTTTAGTAAAATAAAACCTACTTCATTCATTCATTCTAATAAAAATAAAGTTGTAACCACTACATTATTNTTCCACATGCACAAACAAACAAACATTATTCATATGACCACAAGGCCAATGCAAATCAGTTCCATACAGAATGTCGGATAGTTTTAATTGGAGCAACCAGGCCATGCAGCATCTCTTGGTGAAAGTGTTTTAAGAACTGGATTCCTCTCAAAGAAATTGGTTGGCCTCAGCTCAAATGAAGTGCTCAATAGTGGCATTATCGGAAAATCTTCCTGTGCTGGAACATGACGAATTCCAACTACATGCCACATCACTATGTCCTTGTTCACAATCTCTCTATTCCTGCAATTTTAATTAAACAATTTAAACTAGTTAGTAATAAGAATCAAGTTATTAAGAACATTTAATGACAAACTCAACAATAAATAAATAAATAAATAATCATTA
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02472 Ps_contig_mira-and-tgicl-ass_franssen_21349
SEQUENCE=CGTTTTTTTAGTAAAATAAAACCTACTTCATTCATTCATTCTAATAAAAATAAAGTTGTAACCACTACATTATTNTTCCACATGCACAAACAAACAAACATTATTCATATGACCACAAGGCCAATGCAAATCAGTTCCATACAGAATGTCGGATAGTTTTAATTGGAGCAACCAGGCCATGCAGCATCTCTTGGTGAAAGTGTTTTAAGAACTGGATTCCTCTCAAAGAAATTGGTTGGCCTCAGCTCAAATGAAGTGCTCAATAGTGGCATTATCGGAAAATCTTCCTGTGCTGGAACATGACGAATTCCAACTACATGCCACATCACTATGTCCTTGTTCACAATCTCTCTATTCCTGCAATTTTAATTAAACAATTTAAACTAGTTAGTAATAAGAATCAAGTTATTAAGAACATTTAATGACAAACTCAACAATAAATAAATAAATAAATAATCATTA
TARGET=86,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02473 Ps_contig_mira-and-tgicl-ass_franssen_21349
SEQUENCE=CGTTTTTTTAGTAAAATAAAACCTACTTCATTCATTCATTCTAATAAAAATAAAGTTGTAACCACTACATTATTNTTCCACATGCACAAACAAACAAACATTATTCATATGACCACAAGGCCAATGCAAATCAGTTCCATACAGAATGTCGGATAGTTTTAATTGGAGCAACCAGGCCATGCAGCATCTCTTGGTGAAAGTGTTTTAAGAACTGGATTCCTCTCAAAGAAATTGGTTGGCCTCAGCTCAAATGAAGTGCTCAATAGTGGCATTATCGGAAAATCTTCCTGTGCTGGAACATGACGAATTCCAACTACATGCCACATCACTATGTCCTTGTTCACAATCTCTCTATTCCTGCAATTTTAATTAAACAATTTAAACTAGTTAGTAATAAGAATCAAGTTATTAAGAACATTTAATGACAAACTCAACAATAAATAAATAAATAAATAATCATTA
TARGET=436,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02474 Ps_contig_mira-and-tgicl-ass_franssen_21370
SEQUENCE=ACCTAGGTACCATTTGTCACTATTTAGTACAACCAACCTTAAAACTAATAATCTTACAGTTTTATTTGTATAAGTGGCTATGTTANACTTTCTCTACTGACCAAACAAACCACTTCCTTTGCTATCTTGGCTCCACGTTCTTTGGCCATATACAGACCTACGTGATGCATAAACTCCTGGGGATGGGCTAAAGCTCCTTTGATATTTGTGGTTACGTCAAGAGGGACATTGAAGGTAGTACATGCATTCGAATACACCACCACATCTTCTAGAGGCTTCAGAAAACCACGGTTTTTAGCAGACATTGTAGAGCATACAAAATCCAGAACACATATATCTGTGCATACACCTACCACCACCACTGTTTTGATCTTATTCTTCTTCACCCACCACCACCAGTGCATACCTACCCTCATCCTCA
TARGET=387,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9977
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=1.979847
PRIMER_LEFT_SEQUENCE=TACGTGATGCATAAACTCCTGG
PRIMER_RIGHT_SEQUENCE=TGAGGATGAGGGTAGGTATGC
PRIMER_LEFT=158,22
PRIMER_RIGHT=420,21
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=59.020
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=2.0462
PRIMER_LEFT_1_PENALTY=0.066394
PRIMER_RIGHT_1_PENALTY=1.979847
PRIMER_LEFT_1_SEQUENCE=ACCACGGTTTTTAGCAGACATT
PRIMER_RIGHT_1_SEQUENCE=TGAGGATGAGGGTAGGTATGC
PRIMER_LEFT_1=284,22
PRIMER_RIGHT_1=420,21
PRIMER_LEFT_1_TM=59.934
PRIMER_RIGHT_1_TM=59.020
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=2.0754
PRIMER_LEFT_2_PENALTY=0.095538
PRIMER_RIGHT_2_PENALTY=1.979847
PRIMER_LEFT_2_SEQUENCE=TGGCCATATACAGACCTACGTG
PRIMER_RIGHT_2_SEQUENCE=TGAGGATGAGGGTAGGTATGC
PRIMER_LEFT_2=142,22
PRIMER_RIGHT_2=420,21
PRIMER_LEFT_2_TM=59.904
PRIMER_RIGHT_2_TM=59.020
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=2.1964
PRIMER_LEFT_3_PENALTY=0.216588
PRIMER_RIGHT_3_PENALTY=1.979847
PRIMER_LEFT_3_SEQUENCE=CTATCTTGGCTCCACGTTCTTT
PRIMER_RIGHT_3_SEQUENCE=TGAGGATGAGGGTAGGTATGC
PRIMER_LEFT_3=121,22
PRIMER_RIGHT_3=420,21
PRIMER_LEFT_3_TM=59.783
PRIMER_RIGHT_3_TM=59.020
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=2.2546
PRIMER_LEFT_4_PENALTY=0.274768
PRIMER_RIGHT_4_PENALTY=1.979847
PRIMER_LEFT_4_SEQUENCE=ATTTGTGGTTACGTCAAGAGGG
PRIMER_RIGHT_4_SEQUENCE=TGAGGATGAGGGTAGGTATGC
PRIMER_LEFT_4=203,22
PRIMER_RIGHT_4=420,21
PRIMER_LEFT_4_TM=60.275
PRIMER_RIGHT_4_TM=59.020
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_02475 Ps_contig_mira-and-tgicl-ass_franssen_21382
SEQUENCE=CACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTACCTACCTACCAACCAACACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCTACCCCAGTTTACCATTCCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAATCACCTCCTCCACCTTACCACCACTAGATGCCAATCATTGCATCACACTATA
TARGET=56,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0154
PRIMER_LEFT_PENALTY=1.728731
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=ACCCACACCCAGTCTACCACT
PRIMER_RIGHT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT=34,21
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=60.729
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=57.143
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=2.2030
PRIMER_LEFT_1_PENALTY=1.728731
PRIMER_RIGHT_1_PENALTY=0.474295
PRIMER_LEFT_1_SEQUENCE=ACCCACACCCAGTCTACCACT
PRIMER_RIGHT_1_SEQUENCE=TGTATGGCTTCTTGTGTGGTGT
PRIMER_LEFT_1=34,21
PRIMER_RIGHT_1=241,22
PRIMER_LEFT_1_TM=60.729
PRIMER_RIGHT_1_TM=60.474
PRIMER_LEFT_1_GC_PERCENT=57.143
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=2.3127
PRIMER_LEFT_2_PENALTY=1.728731
PRIMER_RIGHT_2_PENALTY=0.583983
PRIMER_LEFT_2_SEQUENCE=ACCCACACCCAGTCTACCACT
PRIMER_RIGHT_2_SEQUENCE=TAAACTGGGGTAGGGAACATGA
PRIMER_LEFT_2=34,21
PRIMER_RIGHT_2=306,22
PRIMER_LEFT_2_TM=60.729
PRIMER_RIGHT_2_TM=60.584
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=2.4105
PRIMER_LEFT_3_PENALTY=2.123796
PRIMER_RIGHT_3_PENALTY=0.286660
PRIMER_LEFT_3_SEQUENCE=CCCACACCCAGTCTACCACT
PRIMER_RIGHT_3_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT_3=35,20
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=59.876
PRIMER_RIGHT_3_TM=59.713
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=2.5548
PRIMER_LEFT_4_PENALTY=2.268186
PRIMER_RIGHT_4_PENALTY=0.286660
PRIMER_LEFT_4_SEQUENCE=ACCCACACCCAGTCTACCAC
PRIMER_RIGHT_4_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT_4=34,20
PRIMER_RIGHT_4=255,22
PRIMER_LEFT_4_TM=59.732
PRIMER_RIGHT_4_TM=59.713
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_02476 Ps_contig_mira-and-tgicl-ass_franssen_21382
SEQUENCE=CACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTACCTACCTACCAACCAACACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCTACCCCAGTTTACCATTCCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAATCACCTCCTCCACCTTACCACCACTAGATGCCAATCATTGCATCACACTATA
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3211
PRIMER_LEFT_PENALTY=0.237199
PRIMER_RIGHT_PENALTY=0.083883
PRIMER_LEFT_SEQUENCE=CCAACCAACACCACATAAGAAA
PRIMER_RIGHT_SEQUENCE=TAGTGAGGGTGAGGGTAGGTGT
PRIMER_LEFT=66,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.763
PRIMER_RIGHT_TM=59.916
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.3211
PRIMER_LEFT_1_PENALTY=0.237199
PRIMER_RIGHT_1_PENALTY=0.083883
PRIMER_LEFT_1_SEQUENCE=CCAACCAACACCACATAAGAAA
PRIMER_RIGHT_1_SEQUENCE=GTGGTAGTGAGGGTGAGGGTAG
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=201,22
PRIMER_LEFT_1_TM=59.763
PRIMER_RIGHT_1_TM=59.916
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=59.091
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.3211
PRIMER_LEFT_2_PENALTY=0.237199
PRIMER_RIGHT_2_PENALTY=0.083883
PRIMER_LEFT_2_SEQUENCE=CCAACCAACACCACATAAGAAA
PRIMER_RIGHT_2_SEQUENCE=AGTGGTAGTGAGGGTGAGGGTA
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=202,22
PRIMER_LEFT_2_TM=59.763
PRIMER_RIGHT_2_TM=59.916
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=0.3211
PRIMER_LEFT_3_PENALTY=0.237199
PRIMER_RIGHT_3_PENALTY=0.083883
PRIMER_LEFT_3_SEQUENCE=CCAACCAACACCACATAAGAAA
PRIMER_RIGHT_3_SEQUENCE=GTAGTGAGGGTGAGGGTAGGTG
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=198,22
PRIMER_LEFT_3_TM=59.763
PRIMER_RIGHT_3_TM=59.916
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=59.091
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=0.3483
PRIMER_LEFT_4_PENALTY=0.186972
PRIMER_RIGHT_4_PENALTY=0.161311
PRIMER_LEFT_4_SEQUENCE=CTACCTACCAACCAACACCACA
PRIMER_RIGHT_4_SEQUENCE=GGAGGAGGAGGAATGGTAAACT
PRIMER_LEFT_4=59,22
PRIMER_RIGHT_4=322,22
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=59.839
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_02477 Ps_contig_mira-and-tgicl-ass_franssen_21382
SEQUENCE=CACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTACCTACCTACCAACCAACACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCTACCCCAGTTTACCATTCCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAATCACCTCCTCCACCTTACCACCACTAGATGCCAATCATTGCATCACACTATA
TARGET=161,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3211
PRIMER_LEFT_PENALTY=0.237199
PRIMER_RIGHT_PENALTY=0.083883
PRIMER_LEFT_SEQUENCE=CCAACCAACACCACATAAGAAA
PRIMER_RIGHT_SEQUENCE=TAGTGAGGGTGAGGGTAGGTGT
PRIMER_LEFT=66,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.763
PRIMER_RIGHT_TM=59.916
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.3211
PRIMER_LEFT_1_PENALTY=0.237199
PRIMER_RIGHT_1_PENALTY=0.083883
PRIMER_LEFT_1_SEQUENCE=CCAACCAACACCACATAAGAAA
PRIMER_RIGHT_1_SEQUENCE=GTGGTAGTGAGGGTGAGGGTAG
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=201,22
PRIMER_LEFT_1_TM=59.763
PRIMER_RIGHT_1_TM=59.916
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=59.091
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.3211
PRIMER_LEFT_2_PENALTY=0.237199
PRIMER_RIGHT_2_PENALTY=0.083883
PRIMER_LEFT_2_SEQUENCE=CCAACCAACACCACATAAGAAA
PRIMER_RIGHT_2_SEQUENCE=AGTGGTAGTGAGGGTGAGGGTA
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=202,22
PRIMER_LEFT_2_TM=59.763
PRIMER_RIGHT_2_TM=59.916
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=0.3211
PRIMER_LEFT_3_PENALTY=0.237199
PRIMER_RIGHT_3_PENALTY=0.083883
PRIMER_LEFT_3_SEQUENCE=CCAACCAACACCACATAAGAAA
PRIMER_RIGHT_3_SEQUENCE=GTAGTGAGGGTGAGGGTAGGTG
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=198,22
PRIMER_LEFT_3_TM=59.763
PRIMER_RIGHT_3_TM=59.916
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=59.091
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=0.3483
PRIMER_LEFT_4_PENALTY=0.186972
PRIMER_RIGHT_4_PENALTY=0.161311
PRIMER_LEFT_4_SEQUENCE=CTACCTACCAACCAACACCACA
PRIMER_RIGHT_4_SEQUENCE=GGAGGAGGAGGAATGGTAAACT
PRIMER_LEFT_4=59,22
PRIMER_RIGHT_4=322,22
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=59.839
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_02478 Ps_contig_mira-and-tgicl-ass_franssen_21382
SEQUENCE=CACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTACCTACCTACCAACCAACACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCTACCCCAGTTTACCATTCCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAATCACCTCCTCCACCTTACCACCACTAGATGCCAATCATTGCATCACACTATA
TARGET=206,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2452
PRIMER_LEFT_PENALTY=0.083883
PRIMER_RIGHT_PENALTY=0.161311
PRIMER_LEFT_SEQUENCE=CTACCCTCACCCTCACTACCAC
PRIMER_RIGHT_SEQUENCE=GGAGGAGGAGGAATGGTAAACT
PRIMER_LEFT=180,22
PRIMER_RIGHT=322,22
PRIMER_LEFT_TM=59.916
PRIMER_RIGHT_TM=59.839
PRIMER_LEFT_GC_PERCENT=59.091
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=143
PRIMER_PAIR_PENALTY_1=0.2452
PRIMER_LEFT_1_PENALTY=0.083883
PRIMER_RIGHT_1_PENALTY=0.161311
PRIMER_LEFT_1_SEQUENCE=CACCTACCCTCACCCTCACTAC
PRIMER_RIGHT_1_SEQUENCE=GGAGGAGGAGGAATGGTAAACT
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=322,22
PRIMER_LEFT_1_TM=59.916
PRIMER_RIGHT_1_TM=59.839
PRIMER_LEFT_1_GC_PERCENT=59.091
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.2452
PRIMER_LEFT_2_PENALTY=0.083883
PRIMER_RIGHT_2_PENALTY=0.161311
PRIMER_LEFT_2_SEQUENCE=TACCCTCACCCTCACTACCACT
PRIMER_RIGHT_2_SEQUENCE=GGAGGAGGAGGAATGGTAAACT
PRIMER_LEFT_2=181,22
PRIMER_RIGHT_2=322,22
PRIMER_LEFT_2_TM=59.916
PRIMER_RIGHT_2_TM=59.839
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.2452
PRIMER_LEFT_3_PENALTY=0.083883
PRIMER_RIGHT_3_PENALTY=0.161311
PRIMER_LEFT_3_SEQUENCE=CTACCCTCACCCTCACTACCAC
PRIMER_RIGHT_3_SEQUENCE=AGGAGGAGGAGGAATGGTAAAC
PRIMER_LEFT_3=180,22
PRIMER_RIGHT_3=323,22
PRIMER_LEFT_3_TM=59.916
PRIMER_RIGHT_3_TM=59.839
PRIMER_LEFT_3_GC_PERCENT=59.091
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=0.2452
PRIMER_LEFT_4_PENALTY=0.083883
PRIMER_RIGHT_4_PENALTY=0.161311
PRIMER_LEFT_4_SEQUENCE=CACCTACCCTCACCCTCACTAC
PRIMER_RIGHT_4_SEQUENCE=AGGAGGAGGAGGAATGGTAAAC
PRIMER_LEFT_4=177,22
PRIMER_RIGHT_4=323,22
PRIMER_LEFT_4_TM=59.916
PRIMER_RIGHT_4_TM=59.839
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_02479 Ps_contig_mira-and-tgicl-ass_franssen_21382
SEQUENCE=CACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTACCTACCTACCAACCAACACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCTACCCCAGTTTACCATTCCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAATCACCTCCTCCACCTTACCACCACTAGATGCCAATCATTGCATCACACTATA
TARGET=252,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2452
PRIMER_LEFT_PENALTY=0.083883
PRIMER_RIGHT_PENALTY=0.161311
PRIMER_LEFT_SEQUENCE=CTACCCTCACCCTCACTACCAC
PRIMER_RIGHT_SEQUENCE=GGAGGAGGAGGAATGGTAAACT
PRIMER_LEFT=180,22
PRIMER_RIGHT=322,22
PRIMER_LEFT_TM=59.916
PRIMER_RIGHT_TM=59.839
PRIMER_LEFT_GC_PERCENT=59.091
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=143
PRIMER_PAIR_PENALTY_1=0.2452
PRIMER_LEFT_1_PENALTY=0.083883
PRIMER_RIGHT_1_PENALTY=0.161311
PRIMER_LEFT_1_SEQUENCE=CACCTACCCTCACCCTCACTAC
PRIMER_RIGHT_1_SEQUENCE=GGAGGAGGAGGAATGGTAAACT
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=322,22
PRIMER_LEFT_1_TM=59.916
PRIMER_RIGHT_1_TM=59.839
PRIMER_LEFT_1_GC_PERCENT=59.091
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.2452
PRIMER_LEFT_2_PENALTY=0.083883
PRIMER_RIGHT_2_PENALTY=0.161311
PRIMER_LEFT_2_SEQUENCE=TACCCTCACCCTCACTACCACT
PRIMER_RIGHT_2_SEQUENCE=GGAGGAGGAGGAATGGTAAACT
PRIMER_LEFT_2=181,22
PRIMER_RIGHT_2=322,22
PRIMER_LEFT_2_TM=59.916
PRIMER_RIGHT_2_TM=59.839
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.2452
PRIMER_LEFT_3_PENALTY=0.083883
PRIMER_RIGHT_3_PENALTY=0.161311
PRIMER_LEFT_3_SEQUENCE=CTACCCTCACCCTCACTACCAC
PRIMER_RIGHT_3_SEQUENCE=AGGAGGAGGAGGAATGGTAAAC
PRIMER_LEFT_3=180,22
PRIMER_RIGHT_3=323,22
PRIMER_LEFT_3_TM=59.916
PRIMER_RIGHT_3_TM=59.839
PRIMER_LEFT_3_GC_PERCENT=59.091
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=0.2452
PRIMER_LEFT_4_PENALTY=0.083883
PRIMER_RIGHT_4_PENALTY=0.161311
PRIMER_LEFT_4_SEQUENCE=CACCTACCCTCACCCTCACTAC
PRIMER_RIGHT_4_SEQUENCE=AGGAGGAGGAGGAATGGTAAAC
PRIMER_LEFT_4=177,22
PRIMER_RIGHT_4=323,22
PRIMER_LEFT_4_TM=59.916
PRIMER_RIGHT_4_TM=59.839
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_02480 Ps_contig_mira-and-tgicl-ass_franssen_21382
SEQUENCE=CACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTACCTACCTACCAACCAACACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCCTACCCCAGTTTACCATTCCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAATCACCTCCTCCACCTTACCACCACTAGATGCCAATCATTGCATCACACTATA
TARGET=312,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6936
PRIMER_LEFT_PENALTY=0.286660
PRIMER_RIGHT_PENALTY=0.406964
PRIMER_LEFT_SEQUENCE=GCCATACAAGTATCCATCACCA
PRIMER_RIGHT_SEQUENCE=TAGTGGTGGTAAGGTGGAGGAG
PRIMER_LEFT=234,22
PRIMER_RIGHT=387,22
PRIMER_LEFT_TM=59.713
PRIMER_RIGHT_TM=60.407
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.7270
PRIMER_LEFT_1_PENALTY=0.340760
PRIMER_RIGHT_1_PENALTY=0.386286
PRIMER_LEFT_1_SEQUENCE=ATGTTCCCTACCCCAGTTTACC
PRIMER_RIGHT_1_SEQUENCE=CAATGATTGGCATCTAGTGGTG
PRIMER_LEFT_1=287,22
PRIMER_RIGHT_1=401,22
PRIMER_LEFT_1_TM=60.341
PRIMER_RIGHT_1_TM=60.386
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=0.7270
PRIMER_LEFT_2_PENALTY=0.340760
PRIMER_RIGHT_2_PENALTY=0.386286
PRIMER_LEFT_2_SEQUENCE=GTTCCCTACCCCAGTTTACCAT
PRIMER_RIGHT_2_SEQUENCE=CAATGATTGGCATCTAGTGGTG
PRIMER_LEFT_2=289,22
PRIMER_RIGHT_2=401,22
PRIMER_LEFT_2_TM=60.341
PRIMER_RIGHT_2_TM=60.386
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=0.7549
PRIMER_LEFT_3_PENALTY=0.368624
PRIMER_RIGHT_3_PENALTY=0.386286
PRIMER_LEFT_3_SEQUENCE=CATGTTCCCTACCCCAGTTTAC
PRIMER_RIGHT_3_SEQUENCE=CAATGATTGGCATCTAGTGGTG
PRIMER_LEFT_3=286,22
PRIMER_RIGHT_3=401,22
PRIMER_LEFT_3_TM=59.631
PRIMER_RIGHT_3_TM=60.386
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=116
PRIMER_PAIR_PENALTY_4=0.7549
PRIMER_LEFT_4_PENALTY=0.368624
PRIMER_RIGHT_4_PENALTY=0.386286
PRIMER_LEFT_4_SEQUENCE=CATGTTCCCTACCCCAGTTTAC
PRIMER_RIGHT_4_SEQUENCE=TGATTGGCATCTAGTGGTGGTA
PRIMER_LEFT_4=286,22
PRIMER_RIGHT_4=398,22
PRIMER_LEFT_4_TM=59.631
PRIMER_RIGHT_4_TM=60.386
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=113
=
PRIMER_SEQUENCE_ID=SSR_02481 Ps_contig_mira-and-tgicl-ass_franssen_21398
SEQUENCE=TCGTCGGGGTACGGCAATAGAGATCGGAAATTCGAACTTCTTTCTCTCTCTCTCTGAAAAATCGTAAAACCGTTTATCTTAGATCGGATCTATTCGACGTCGTTTCGGTAGGAATGGCTCCATTCAGCGGCGATGAAACGGCACCGTTTTTTGGCTTCCTTGGAGCCGCAGCTGCTCTCGTCTTCTCATGTATGGGAGCYGCGTATGGTACTGCAAAAAGTGGTGTGGGAGTGGCTTCAATGGGTGTGATGAGACCGGAGCTGGTGATGAAGTCGATTGTGCCGGTTTGTTATGGCTGGGTTGGTTTTTGGGTTATTTTATGGTTCTTTATTTATTGCTTGTTTATTTATCAGTACGAGGGACTTAAACCCAAAGGCCAAATTCTTTATTTACCTTTTTGATTGGTTATGCACATCTCTCCTNTGTCTTGCTTCGG
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02482 Ps_contig_mira-and-tgicl-ass_franssen_21407
SEQUENCE=CGTGTCTCAGAAGCAGTGGTATCTAACGTCTAGAGTACGKCGGGGGTTGGTAAAGAGGTTGTTGTTGTGGGGATAAGATTGCAGTGAGTGAGTGTAGTTTGTAGAAACTAGTGGTCTTCTGTCTTCAGCTATTCTTATTTACAAATCATCATCTCTTTGTTCTT
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02483 Ps_contig_mira-and-tgicl-ass_franssen_21467
SEQUENCE=TCGGGGATATCATAATATCTCTTTTCTTTTTCTTTCTTTCATTAAACATTTAGTTTCTTGTCAAAAAATGGTGCTAGCTGGTAAGCTTATTACAGAACTTGGTATCAAAACACCAGCTGGGGACAAGTTCTTCAAACTGTTTGCATCAGAACTTCACGAAGTGACTAAAACCTTTGTAGAAAGAATTCATCATAACCAAGTATTCACCATGGTAGAA
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02484 Ps_contig_mira-and-tgicl-ass_franssen_21477
SEQUENCE=GTCCCAAAACTACAAAAATAGTCTTTAATCCAAGGAACTTAACTTTGTTGAATATCTCATACAAATAAACTATACATCATCATCATCATCATCATCATYAYYTTAATWAAACAATAAAAGGGAACGAAAGAAAAGAACAAAWTWAAAACAAACATAAACGAACAACATAGTAATTCTTTAATTAAACCTAAAAGAAAACTAAGGATCATAACCATTGACTAAGAAGTAGGACCGAACG
TARGET=75,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02485 Ps_contig_mira-and-tgicl-ass_franssen_21484
SEQUENCE=CGGGTTTTACTTTTTCTKAAACTAAACACAAACACAGTCTCTCTCTCTTTTCACCTATCGCGTCCCTCTTTCTCTCGGGCTCTCACTGCGTCAAAAACCNCGAGAAGAGGAACAACATGCAGATCTTCGTCAAGACTTTAACCGGAAAACCTCACTCTCGAGGTTGAGAGCAGCGACACCATCGAACACGTCAAGGCCAAGNTCCAGGACAAGGAGTCTCCCTCTGACCAGCAG
TARGET=38,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02486 Ps_contig_mira-and-tgicl-ass_franssen_21512
SEQUENCE=AGTAGCCACTAAGAAAAAGCCCAAAGAAAAACAACAAAGTAAAAAAATAAAAGTGTTTCTAATCTTTGTTTATGTGTATCTTGTGAACTGTGTTTGTGTGTAGTAAATAATGAAATAAATAAATTAAAACTAGATAATAATTTAA
TARGET=113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02487 Ps_contig_mira-and-tgicl-ass_franssen_21533
SEQUENCE=TTTTAWGTAGTAATAGAGTGAATGTTATATATTCTTGAACCAACATAGCATACATCATAGAATTATTTTTTCTGAACCATACATGATTATCTTATAAAAGTACTTAAGAGTTTACAAAGTAAAACGATATACATAATTTATAACAAACAAACACTATTATTGTATAAAAGAAACGTGAAATCAAAAGTATTGGNGTGTAGAAGAACAAGAAAGTAAAAACATGTCTAGACGGAGGAACACCTATTCGA
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02488 Ps_contig_mira-and-tgicl-ass_franssen_21555
SEQUENCE=ACGTAAGGATTGTTTTGACGGATATGTTGGCTTTTTCAATGGAAGAAGATGGTTCAAATGTTTTTGTAGATTGGGTGAAGAAGAATAAGATCAAAACAGTGGTGGTGGTAGGTGTATGCACAGATACATGTGTTCTGGATTTTGTATGCTCTACAATGTCTGCTAAAAACCGTGGTTTTCTGAAGCCTGTGGTTGTTTGTTCCTTCCTTCTCTCTCTCTCACTGTCTCACTTCCTCAACCAAACCTAATCCTTCACATCGCAATCAA
TARGET=209,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2236
PRIMER_LEFT_PENALTY=0.207335
PRIMER_RIGHT_PENALTY=0.016312
PRIMER_LEFT_SEQUENCE=CAGTGGTGGTGGTAGGTGTATG
PRIMER_RIGHT_SEQUENCE=GGTTTGGTTGAGGAAGTGAGAC
PRIMER_LEFT=96,22
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=60.207
PRIMER_RIGHT_TM=60.016
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=0.2530
PRIMER_LEFT_1_PENALTY=0.236697
PRIMER_RIGHT_1_PENALTY=0.016312
PRIMER_LEFT_1_SEQUENCE=CGTAAGGATTGTTTTGACGGAT
PRIMER_RIGHT_1_SEQUENCE=GGTTTGGTTGAGGAAGTGAGAC
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=244,22
PRIMER_LEFT_1_TM=60.237
PRIMER_RIGHT_1_TM=60.016
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.8000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.2591
PRIMER_LEFT_2_PENALTY=0.242742
PRIMER_RIGHT_2_PENALTY=0.016312
PRIMER_LEFT_2_SEQUENCE=GATCAAAACAGTGGTGGTGGTA
PRIMER_RIGHT_2_SEQUENCE=GGTTTGGTTGAGGAAGTGAGAC
PRIMER_LEFT_2=88,22
PRIMER_RIGHT_2=244,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=60.016
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.2603
PRIMER_LEFT_3_PENALTY=0.243965
PRIMER_RIGHT_3_PENALTY=0.016312
PRIMER_LEFT_3_SEQUENCE=GTTTTGACGGATATGTTGGCTT
PRIMER_RIGHT_3_SEQUENCE=GGTTTGGTTGAGGAAGTGAGAC
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=244,22
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=60.016
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.3030
PRIMER_LEFT_4_PENALTY=0.207335
PRIMER_RIGHT_4_PENALTY=0.095629
PRIMER_LEFT_4_SEQUENCE=CAGTGGTGGTGGTAGGTGTATG
PRIMER_RIGHT_4_SEQUENCE=TGATTGCGATGTGAAGGATTAG
PRIMER_LEFT_4=96,22
PRIMER_RIGHT_4=265,22
PRIMER_LEFT_4_TM=60.207
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_02489 Ps_contig_mira-and-tgicl-ass_franssen_21587
SEQUENCE=TTAAGGAATATTAATTTTATGCAGAAAATAAAAAGTTAGTTCATGGAGATTCAAGAACTTAGANGCTTTTAATTTTTTAAWTTGGAACTTTGTTTTTTATTTTATTTTAATATTAGTAGTATGGTAGCTCAATGTGGAGCATGTGGATGATGTGATGTATGGTATTTCATGTGGTGCATGATGGCATGTAGTGACAATGTTTATGATTATTWGAWRCTMAWARAAAAAACGT
TARGET=95,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02490 Ps_contig_mira-and-tgicl-ass_franssen_21597
SEQUENCE=ACCCGACTCGTTGTTTTTTGTTAGGATGTTGACCTTGATAAGGTTGGTCTGAGTGGACTGTCTTTGGTCTGTTTCTTTTACTTAATGGTCAGATATGCAGTGCAACATCCCGACTTATTGTGCACGAAAGTATAGCCGTAGAATTTGTTGGATAAACTTGTCAAAATGGGCCGAAAACATCAAAATTTCGGATCCGTTGGAAGAAAGGTTGCAGGCTTGGACCTATTGTCAGTGAAGCAACAAGTAATAAGAAAGTATTGAATTGCATCTCATCGGCTAAGAGTGAGGGTGCAACAATTTTGACTGGTGGCCGTCGACCCGAGCATTTAAAAGGAAGGGATTATTTTTGTTGAAACCCAACCATCAATAAACCGATGTAGACCCACCTCGATGACTAAAGTTTGGAAGAAGAAGAAGTTATTTCGGACCTGTACTCG
TARGET=405,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02491 Ps_contig_mira-and-tgicl-ass_franssen_21617
SEQUENCE=GTCGGGKATCATCCAAACAACAACAACACTCTCATCATCGTCTCATACTTTACCTTTCATTTCAGAGTTACAGCTACATAATCCATTTTAGTATAGTTAATTAATTTCCAGTTCGATCCATCTTGTTAGTAGTGATCATCATGGCTGAGGANAATCAGAACAAGTACGAGGANACTACATCCCGACAAACCCAACTTCTGGAACGGAACAGGAAGATACGAAAGGACAGGTTAGG
TARGET=16,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02492 Ps_contig_mira-and-tgicl-ass_franssen_21620
SEQUENCE=GGCGATCCAATCCTTACCACTACTAGATAGTTTGGTCTTACCGTAGTATCCCATGGATTTGAAGAATATATATATAATAATATTGGAAATAAAGAGAACATAGTGCAAAGCAGGAAGATGCATTTCAAAATATACAAGGAAAAAACAACGGAAGGAAAGTTTCCATGCACTTGGTCTCAAGAAAAGTCATTATTCTTTCCCATTTTGTTTTGTTATGAATTGYTATTCTCATTATTGGAGTGCTTAGGTTTTTGAATTTAGACAATTGTAAGGCACTGTGTGTGTAATATCAATAAACGAATAAAGTCTTTTTATTTGACAAAAACTAAAAAAAA
TARGET=66,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02493 Ps_contig_mira-and-tgicl-ass_franssen_21621
SEQUENCE=TTTTTTGKAMTAAAAACATTATTTCTTTGGATCCATTATTTCTAACATATAATCAGCCACATACAGGTTCTTCTGCTAGAGTAATTTACATTTAGATAAAGTAAAGGTAGGGTAAACTTAAAACCAAACCAAACGAAAGTAAAGTAAAGTAAAGGGTAGGGACGACGAAAGGTAAGTTAAGTTTAGGTTACGTCGTAAGGAAA
TARGET=136,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02494 Ps_contig_mira-and-tgicl-ass_franssen_21630
SEQUENCE=TTTAGAGATAAAACACTTTATTTTATTACTATTATTACCAAAACATACACATACACTTATTATATTATATTATATGTATATGTTTGGAAAGCCCATCAAAGACTGATAGTAATGATTTAATTTCCAATCCTGGCTCCAGTGTTGATCTTGGCCCTTTTTACCCTAAAAAATTTCCTTGGAAGTTTCCCTT
TARGET=58,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02495 Ps_contig_mira-and-tgicl-ass_franssen_21633
SEQUENCE=ATTGCCCACGAGTTTGGTCACAAGGTGACTTTGTATGAAGCACCGTCGGAAACCAAAGTTGAAGAGGAAGGTGAAAAGAAACACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTCAAGTGAAGAGGAAGGAGAAGATGGTGAGAAAAGAAAGAAGAAGAAGGAAANAGAAGAAAGAAGAACACTACGGTGCAGTGAGAAGTGAGTGTGAGAACGACGAGAAGAGGTT
TARGET=169,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1385
PRIMER_LEFT_PENALTY=0.114470
PRIMER_RIGHT_PENALTY=0.023981
PRIMER_LEFT_SEQUENCE=GGAAGGTGAAAAGAAACACACC
PRIMER_RIGHT_SEQUENCE=ACTCACTTCTCACTGCACCGTA
PRIMER_LEFT=65,22
PRIMER_RIGHT=223,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=159
PRIMER_PAIR_PENALTY_1=0.1385
PRIMER_LEFT_1_PENALTY=0.114470
PRIMER_RIGHT_1_PENALTY=0.023981
PRIMER_LEFT_1_SEQUENCE=GGAAGGTGAAAAGAAACACACC
PRIMER_RIGHT_1_SEQUENCE=TCACTTCTCACTGCACCGTAGT
PRIMER_LEFT_1=65,22
PRIMER_RIGHT_1=221,22
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=5.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=0.1414
PRIMER_LEFT_2_PENALTY=0.117452
PRIMER_RIGHT_2_PENALTY=0.023981
PRIMER_LEFT_2_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_RIGHT_2_SEQUENCE=TCTCACTGCACCGTAGTGTTCT
PRIMER_LEFT_2=95,22
PRIMER_RIGHT_2=216,22
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=0.1414
PRIMER_LEFT_3_PENALTY=0.117452
PRIMER_RIGHT_3_PENALTY=0.023981
PRIMER_LEFT_3_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_RIGHT_3_SEQUENCE=ACTCACTTCTCACTGCACCGTA
PRIMER_LEFT_3=95,22
PRIMER_RIGHT_3=223,22
PRIMER_LEFT_3_TM=60.117
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.1414
PRIMER_LEFT_4_PENALTY=0.117452
PRIMER_RIGHT_4_PENALTY=0.023981
PRIMER_LEFT_4_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_RIGHT_4_SEQUENCE=TCACTTCTCACTGCACCGTAGT
PRIMER_LEFT_4=95,22
PRIMER_RIGHT_4=221,22
PRIMER_LEFT_4_TM=60.117
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=127
=
PRIMER_SEQUENCE_ID=SSR_02496 Ps_contig_mira-and-tgicl-ass_franssen_21692
SEQUENCE=AACAATACCAGGATGCCATTGCCAAGGAGGATGATGAGTATGAAGGAGAAGAAGGTGAGGACCAGTATGTGGAGCATTAGCTGAATGAAACTCTCGGGTAATGCACTAATGGTAAAAATGTCCACTATGCAACTTTGCTGTTATTTGTGTATCTACAGCCGTATATGCCCTTTTTTTATTCTCAATTTTACCCGTTTCTTTACATTTTGCCAATTTCATCTTTTAAGTGTATTATTATTATTATCATTTCTGAGTTGTTTGGAAATTGGATTGTGAATTGTCAGACAGGGTTATTCTATACTTCTATTCCTTTAATCATACG
TARGET=230,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5136
PRIMER_LEFT_PENALTY=0.009871
PRIMER_RIGHT_PENALTY=0.503696
PRIMER_LEFT_SEQUENCE=TCTACAGCCGTATATGCCCTTT
PRIMER_RIGHT_SEQUENCE=AACCCTGTCTGACAATTCACAA
PRIMER_LEFT=151,22
PRIMER_RIGHT=291,22
PRIMER_LEFT_TM=60.010
PRIMER_RIGHT_TM=59.496
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.5314
PRIMER_LEFT_1_PENALTY=0.027712
PRIMER_RIGHT_1_PENALTY=0.503696
PRIMER_LEFT_1_SEQUENCE=GAATGAAACTCTCGGGTAATGC
PRIMER_RIGHT_1_SEQUENCE=AACCCTGTCTGACAATTCACAA
PRIMER_LEFT_1=82,22
PRIMER_RIGHT_1=291,22
PRIMER_LEFT_1_TM=59.972
PRIMER_RIGHT_1_TM=59.496
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.5563
PRIMER_LEFT_2_PENALTY=0.009871
PRIMER_RIGHT_2_PENALTY=0.546410
PRIMER_LEFT_2_SEQUENCE=TCTACAGCCGTATATGCCCTTT
PRIMER_RIGHT_2_SEQUENCE=CCTGTCTGACAATTCACAATCC
PRIMER_LEFT_2=151,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=60.010
PRIMER_RIGHT_2_TM=59.454
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=138
PRIMER_PAIR_PENALTY_3=0.5563
PRIMER_LEFT_3_PENALTY=0.009871
PRIMER_RIGHT_3_PENALTY=0.546410
PRIMER_LEFT_3_SEQUENCE=TCTACAGCCGTATATGCCCTTT
PRIMER_RIGHT_3_SEQUENCE=CCCTGTCTGACAATTCACAATC
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=289,22
PRIMER_LEFT_3_TM=60.010
PRIMER_RIGHT_3_TM=59.454
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=0.5741
PRIMER_LEFT_4_PENALTY=0.027712
PRIMER_RIGHT_4_PENALTY=0.546410
PRIMER_LEFT_4_SEQUENCE=GAATGAAACTCTCGGGTAATGC
PRIMER_RIGHT_4_SEQUENCE=CCTGTCTGACAATTCACAATCC
PRIMER_LEFT_4=82,22
PRIMER_RIGHT_4=288,22
PRIMER_LEFT_4_TM=59.972
PRIMER_RIGHT_4_TM=59.454
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_02497 Ps_contig_mira-and-tgicl-ass_franssen_21725
SEQUENCE=GTCGGKATCCAAACAACAACAACACTATCATCATCGTCTCATACTTTACCTTTCATTTCAGAGTTACAGCTACATAATCCATTTTAGTTACAGTTAATTAATTTCCAGTTCGATCCATCTTGTTAGTAGTGATCATCATGGCTGAGGAAGAATCAAGAACAAGTACGAGAACTCCNCCGACAAACCCAACTCTGAGACAGAGATACGAAAGGACAGGTAGGTTTGTTTTTAGATTTTTCCGG
TARGET=12,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02498 Ps_contig_mira-and-tgicl-ass_franssen_21766
SEQUENCE=GTKTTGGTTAAGTTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAAGAAAATTGGAGGGATACGTTCTTCAATGGACGAAACAGAAGAAATACTCTGGTGTTGTTGTATGGATTAGGTGTTGAGACAGAACTAATAGAAAGAAAGAATATAGTTGGTT
TARGET=167,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02499 Ps_contig_mira-and-tgicl-ass_franssen_21768
SEQUENCE=GTTAATTTAAACAAACAAACATTATTATTAGCACTCTGGAGAACAAAAACAAACATGCTAACTTATTTGCAAGAACTACACAACCTCTATATACATCAAACACACACAACACACACAACACACAAAGMMAAACTGCGTTGGATACGGCTTGAGACCAAAACGAAAACGAGCACACAATCGATCCAATGATCTTCACTATCACTAACTAGCTTCACTTATCACA
TARGET=9,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02500 Ps_contig_mira-and-tgicl-ass_franssen_21774
SEQUENCE=CAACCTCATTGTTGGAAACACCGCCACCGTTGTTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAGCCTCTCACCTTACCGGCACGAGCGAGTGATCCATGAACCTTACCCATGATTGCGAAGATGAATTCTCTGCTGAATCCTCTCTTCAATCGCAGCAGACTAGGAAAGGCACAAACGC
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5972
PRIMER_LEFT_PENALTY=1.543888
PRIMER_RIGHT_PENALTY=0.053348
PRIMER_LEFT_SEQUENCE=CAACCTCATTGTTGGAAACACC
PRIMER_RIGHT_SEQUENCE=ATGGGTAAGGTTCATGGATCAC
PRIMER_LEFT=0,22
PRIMER_RIGHT=196,22
PRIMER_LEFT_TM=61.544
PRIMER_RIGHT_TM=59.947
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=1.6950
PRIMER_LEFT_1_PENALTY=1.543888
PRIMER_RIGHT_1_PENALTY=0.151106
PRIMER_LEFT_1_SEQUENCE=CAACCTCATTGTTGGAAACACC
PRIMER_RIGHT_1_SEQUENCE=TAAGGTGAGAGGCTACGGTCAT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=161,22
PRIMER_LEFT_1_TM=61.544
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=1.8465
PRIMER_LEFT_2_PENALTY=1.543888
PRIMER_RIGHT_2_PENALTY=0.302618
PRIMER_LEFT_2_SEQUENCE=CAACCTCATTGTTGGAAACACC
PRIMER_RIGHT_2_SEQUENCE=GTTTGTGCCTTTCCTAGTCTGC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=61.544
PRIMER_RIGHT_2_TM=60.303
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=1.8477
PRIMER_LEFT_3_PENALTY=1.543888
PRIMER_RIGHT_3_PENALTY=0.303792
PRIMER_LEFT_3_SEQUENCE=CAACCTCATTGTTGGAAACACC
PRIMER_RIGHT_3_SEQUENCE=GGGTAAGGTTCATGGATCACTC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=194,22
PRIMER_LEFT_3_TM=61.544
PRIMER_RIGHT_3_TM=59.696
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=1.9683
PRIMER_LEFT_4_PENALTY=1.543888
PRIMER_RIGHT_4_PENALTY=0.424392
PRIMER_LEFT_4_SEQUENCE=CAACCTCATTGTTGGAAACACC
PRIMER_RIGHT_4_SEQUENCE=TTTGTGCCTTTCCTAGTCTGCT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=261,22
PRIMER_LEFT_4_TM=61.544
PRIMER_RIGHT_4_TM=60.424
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_02501 Ps_contig_mira-and-tgicl-ass_franssen_21774
SEQUENCE=CAACCTCATTGTTGGAAACACCGCCACCGTTGTTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAGCCTCTCACCTTACCGGCACGAGCGAGTGATCCATGAACCTTACCCATGATTGCGAAGATGAATTCTCTGCTGAATCCTCTCTTCAATCGCAGCAGACTAGGAAAGGCACAAACGC
TARGET=84,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5972
PRIMER_LEFT_PENALTY=1.543888
PRIMER_RIGHT_PENALTY=0.053348
PRIMER_LEFT_SEQUENCE=CAACCTCATTGTTGGAAACACC
PRIMER_RIGHT_SEQUENCE=ATGGGTAAGGTTCATGGATCAC
PRIMER_LEFT=0,22
PRIMER_RIGHT=196,22
PRIMER_LEFT_TM=61.544
PRIMER_RIGHT_TM=59.947
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=1.6950
PRIMER_LEFT_1_PENALTY=1.543888
PRIMER_RIGHT_1_PENALTY=0.151106
PRIMER_LEFT_1_SEQUENCE=CAACCTCATTGTTGGAAACACC
PRIMER_RIGHT_1_SEQUENCE=TAAGGTGAGAGGCTACGGTCAT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=161,22
PRIMER_LEFT_1_TM=61.544
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=1.8465
PRIMER_LEFT_2_PENALTY=1.543888
PRIMER_RIGHT_2_PENALTY=0.302618
PRIMER_LEFT_2_SEQUENCE=CAACCTCATTGTTGGAAACACC
PRIMER_RIGHT_2_SEQUENCE=GTTTGTGCCTTTCCTAGTCTGC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=61.544
PRIMER_RIGHT_2_TM=60.303
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=1.8477
PRIMER_LEFT_3_PENALTY=1.543888
PRIMER_RIGHT_3_PENALTY=0.303792
PRIMER_LEFT_3_SEQUENCE=CAACCTCATTGTTGGAAACACC
PRIMER_RIGHT_3_SEQUENCE=GGGTAAGGTTCATGGATCACTC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=194,22
PRIMER_LEFT_3_TM=61.544
PRIMER_RIGHT_3_TM=59.696
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=1.9683
PRIMER_LEFT_4_PENALTY=1.543888
PRIMER_RIGHT_4_PENALTY=0.424392
PRIMER_LEFT_4_SEQUENCE=CAACCTCATTGTTGGAAACACC
PRIMER_RIGHT_4_SEQUENCE=TTTGTGCCTTTCCTAGTCTGCT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=261,22
PRIMER_LEFT_4_TM=61.544
PRIMER_RIGHT_4_TM=60.424
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_02502 Ps_contig_mira-and-tgicl-ass_franssen_21837
SEQUENCE=CACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTTAAGNGAAGAGGAAGGAGAAGATGGTGAAGAAAAGAAGAAGAAGAAGGAAANAGAAGAAGAAGAAMWCGTRCRGTRMGACMGTACGTTACGGAAGGTAAAGGTTAGGTTAAGGTTAGGTTTAGGTAAAGGTAAACCGGTAAACCGGGTTAACCGGTAACGGTAAAGGGTAAAAAGGTAACGGGTTTAACCGTTACGTAAACGTAAAAAGGTTAAAGGTAAAGGTAACGTACCGGTTACCGGTAACGGACGAACGAAACGAAACGAACGAAGGAAGTAACAAC
TARGET=85,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02503 Ps_contig_mira-and-tgicl-ass_franssen_21837
SEQUENCE=CACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTTAAGNGAAGAGGAAGGAGAAGATGGTGAAGAAAAGAAGAAGAAGAAGGAAANAGAAGAAGAAGAAMWCGTRCRGTRMGACMGTACGTTACGGAAGGTAAAGGTTAGGTTAAGGTTAGGTTTAGGTAAAGGTAAACCGGTAAACCGGGTTAACCGGTAACGGTAAAGGGTAAAAAGGTAACGGGTTTAACCGTTACGTAAACGTAAAAAGGTTAAAGGTAAAGGTAACGTACCGGTTACCGGTAACGGACGAACGAAACGAAACGAACGAAGGAAGTAACAAC
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02504 Ps_contig_mira-and-tgicl-ass_franssen_21837
SEQUENCE=CACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTTAAGNGAAGAGGAAGGAGAAGATGGTGAAGAAAAGAAGAAGAAGAAGGAAANAGAAGAAGAAGAAMWCGTRCRGTRMGACMGTACGTTACGGAAGGTAAAGGTTAGGTTAAGGTTAGGTTTAGGTAAAGGTAAACCGGTAAACCGGGTTAACCGGTAACGGTAAAGGGTAAAAAGGTAACGGGTTTAACCGTTACGTAAACGTAAAAAGGTTAAAGGTAAAGGTAACGTACCGGTTACCGGTAACGGACGAACGAAACGAAACGAACGAAGGAAGTAACAAC
TARGET=303,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02505 Ps_contig_mira-and-tgicl-ass_franssen_21852
SEQUENCE=AAATGAAGTAAATGATGCCAANATTACGGTGGAGGTTCTGATCATGGCCATGGTCATGGACATGGAGGCTACGGTCATGGAGGCTACGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTAGAAAAGTCAAATGAAGTAAATGATGACCAAAATAGTGGGCGGTGGAC
TARGET=139,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4965
PRIMER_LEFT_PENALTY=0.414737
PRIMER_RIGHT_PENALTY=0.081801
PRIMER_LEFT_SEQUENCE=CAANATTACGGTGGAGGTTCTG
PRIMER_RIGHT_SEQUENCE=GCCCACTATTTTGGTCATCATT
PRIMER_LEFT=18,22
PRIMER_RIGHT=274,22
PRIMER_LEFT_TM=60.415
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.50
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.75
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.5546
PRIMER_LEFT_1_PENALTY=0.472769
PRIMER_RIGHT_1_PENALTY=0.081801
PRIMER_LEFT_1_SEQUENCE=AANATTACGGTGGAGGTTCTGA
PRIMER_RIGHT_1_SEQUENCE=GCCCACTATTTTGGTCATCATT
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=274,22
PRIMER_LEFT_1_TM=59.527
PRIMER_RIGHT_1_TM=60.082
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.50
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=1.1951
PRIMER_LEFT_2_PENALTY=1.113257
PRIMER_RIGHT_2_PENALTY=0.081801
PRIMER_LEFT_2_SEQUENCE=CCAANATTACGGTGGAGGTTCT
PRIMER_RIGHT_2_SEQUENCE=GCCCACTATTTTGGTCATCATT
PRIMER_LEFT_2=17,22
PRIMER_RIGHT_2=274,22
PRIMER_LEFT_2_TM=61.113
PRIMER_RIGHT_2_TM=60.082
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.50
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.75
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=1.2831
PRIMER_LEFT_3_PENALTY=1.201304
PRIMER_RIGHT_3_PENALTY=0.081801
PRIMER_LEFT_3_SEQUENCE=AANATTACGGTGGAGGTTCTGAT
PRIMER_RIGHT_3_SEQUENCE=GCCCACTATTTTGGTCATCATT
PRIMER_LEFT_3=19,23
PRIMER_RIGHT_3=274,22
PRIMER_LEFT_3_TM=59.799
PRIMER_RIGHT_3_TM=60.082
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.50
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=1.3254
PRIMER_LEFT_4_PENALTY=1.243575
PRIMER_RIGHT_4_PENALTY=0.081801
PRIMER_LEFT_4_SEQUENCE=CCAANATTACGGTGGAGGTTC
PRIMER_RIGHT_4_SEQUENCE=GCCCACTATTTTGGTCATCATT
PRIMER_LEFT_4=17,21
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=60.244
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.50
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_02506 Ps_contig_mira-and-tgicl-ass_franssen_21852
SEQUENCE=AAATGAAGTAAATGATGCCAANATTACGGTGGAGGTTCTGATCATGGCCATGGTCATGGACATGGAGGCTACGGTCATGGAGGCTACGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTAGAAAAGTCAAATGAAGTAAATGATGACCAAAATAGTGGGCGGTGGAC
TARGET=163,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4965
PRIMER_LEFT_PENALTY=0.414737
PRIMER_RIGHT_PENALTY=0.081801
PRIMER_LEFT_SEQUENCE=CAANATTACGGTGGAGGTTCTG
PRIMER_RIGHT_SEQUENCE=GCCCACTATTTTGGTCATCATT
PRIMER_LEFT=18,22
PRIMER_RIGHT=274,22
PRIMER_LEFT_TM=60.415
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.50
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.75
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.5546
PRIMER_LEFT_1_PENALTY=0.472769
PRIMER_RIGHT_1_PENALTY=0.081801
PRIMER_LEFT_1_SEQUENCE=AANATTACGGTGGAGGTTCTGA
PRIMER_RIGHT_1_SEQUENCE=GCCCACTATTTTGGTCATCATT
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=274,22
PRIMER_LEFT_1_TM=59.527
PRIMER_RIGHT_1_TM=60.082
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.50
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=1.1951
PRIMER_LEFT_2_PENALTY=1.113257
PRIMER_RIGHT_2_PENALTY=0.081801
PRIMER_LEFT_2_SEQUENCE=CCAANATTACGGTGGAGGTTCT
PRIMER_RIGHT_2_SEQUENCE=GCCCACTATTTTGGTCATCATT
PRIMER_LEFT_2=17,22
PRIMER_RIGHT_2=274,22
PRIMER_LEFT_2_TM=61.113
PRIMER_RIGHT_2_TM=60.082
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.50
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.75
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=1.2831
PRIMER_LEFT_3_PENALTY=1.201304
PRIMER_RIGHT_3_PENALTY=0.081801
PRIMER_LEFT_3_SEQUENCE=AANATTACGGTGGAGGTTCTGAT
PRIMER_RIGHT_3_SEQUENCE=GCCCACTATTTTGGTCATCATT
PRIMER_LEFT_3=19,23
PRIMER_RIGHT_3=274,22
PRIMER_LEFT_3_TM=59.799
PRIMER_RIGHT_3_TM=60.082
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.50
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=1.3254
PRIMER_LEFT_4_PENALTY=1.243575
PRIMER_RIGHT_4_PENALTY=0.081801
PRIMER_LEFT_4_SEQUENCE=CCAANATTACGGTGGAGGTTC
PRIMER_RIGHT_4_SEQUENCE=GCCCACTATTTTGGTCATCATT
PRIMER_LEFT_4=17,21
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=60.244
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.50
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_02507 Ps_contig_mira-and-tgicl-ass_franssen_21876
SEQUENCE=GTTCGGGGTTTRWTCTTTCTTCATATCTCTCTCACTTTCACTCCCAACCAAAACACACACACATCATGGGTTTGCAGGAGGTATTTTGAGGAGCATGCTAGCGAAAGTTAAGACTCTAACAGAGAGTCCATCAAATGAAAACTTGTCTTATCTTATGGATTGTGACAAGACAACGACCCTACCGTTCTTACGGGACCCGTTACGTTTACCCGACCCGT
TARGET=53,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02508 Ps_contig_mira-and-tgicl-ass_franssen_21880
SEQUENCE=AACCAAACCTTACGGTATCAAGAAGTGTTTAGTTCCTCACTTTCTTTAGTCCCCACGTAARCTTCTGAACCTGTTTTGTCAATCTGAGCCAAATGATGTGACTCCAACTTCACAACCGAAGTCACAACCACAAGCTCTGTATCAGTTAAAGTATTGTAAAMAAATTCAAACTCCTTAATAATGTCGATGATCATGTCGATCCTCTTCGAGTCGATCAAGACGTTAAGGAAGTTGTGTGTGTGTGGTTGGAAGCCTGAGGTAGTGGCGAACTCTCCTATAAGTTGGCGTTTGGTGGAATCCTGAGACACGCAACG
TARGET=233,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02509 Ps_contig_mira-and-tgicl-ass_franssen_21912
SEQUENCE=CGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCGCCATGACCGCAACCTCCACCACCATGACCGTAGCCTCCATGTCCATGACCRTAGCCTCCATGTCCATGACCATAGCCATGACCATAACCCTCCACCGTAATATTTGGCATCATTTTACTTCATTTGTACTTTTCAGCAACTTCCTTCTTTGGCATTAGTGGAAGTCTCAGCTAATTCTCTAGCTGAAACCTCGGAAGGAAATAAACAAGAACCA
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02510 Ps_contig_mira-and-tgicl-ass_franssen_21913
SEQUENCE=TCCCATAATAGAGTACAGCATAAAACCCCAAACATTACATATCATCATGTTCATCATCATCATCACATATAACGATAGTAATAATAATTAGAATTCAACTTTTTAACTTCAAAC
TARGET=51,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02511 Ps_contig_mira-and-tgicl-ass_franssen_21916
SEQUENCE=GCTGTAGATGCTCCCGAAACCCAAGATCCTACTTGAAGTTGAGAACAAGGAAGTGGTAACAGAGGAAGCAAAGGAAGAGAACTTAGAAGCACCTAAAGAAACTGAGGAATCGGTTGAAGAAGTGAAGGAAGAGGCGGAAGCAGTAGTGGAGGAGCCAATAGCAACTACTGAAAATGAGTCAGCACCACCACCACCAGCAACTGCAGAGGATGTGATTGAAGAAACAAAACAGTTAGAATCAGGTTAGGGAAGCCTACCGAGCAACGG
TARGET=183,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1355
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.133226
PRIMER_LEFT_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_RIGHT_SEQUENCE=GGTAGGCTTCCCTAACCTGATT
PRIMER_LEFT=113,22
PRIMER_RIGHT=257,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.867
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=0.2427
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.133226
PRIMER_LEFT_1_SEQUENCE=AATCGGTTGAAGAAGTGAAGGA
PRIMER_RIGHT_1_SEQUENCE=GGTAGGCTTCCCTAACCTGATT
PRIMER_LEFT_1=107,22
PRIMER_RIGHT_1=257,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=0.2427
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.133226
PRIMER_LEFT_2_SEQUENCE=GAATCGGTTGAAGAAGTGAAGG
PRIMER_RIGHT_2_SEQUENCE=GGTAGGCTTCCCTAACCTGATT
PRIMER_LEFT_2=106,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.2565
PRIMER_LEFT_3_PENALTY=0.123278
PRIMER_RIGHT_3_PENALTY=0.133226
PRIMER_LEFT_3_SEQUENCE=CAGAGGAAGCAAAGGAAGAGAA
PRIMER_RIGHT_3_SEQUENCE=GGTAGGCTTCCCTAACCTGATT
PRIMER_LEFT_3=59,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=60.123
PRIMER_RIGHT_3_TM=59.867
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=199
PRIMER_PAIR_PENALTY_4=0.2620
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.259704
PRIMER_LEFT_4_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_RIGHT_4_SEQUENCE=TTCAATCACATCCTCTGCAGTT
PRIMER_LEFT_4=113,22
PRIMER_RIGHT_4=219,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=59.740
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=107
=
PRIMER_SEQUENCE_ID=SSR_02512 Ps_contig_mira-and-tgicl-ass_franssen_21919
SEQUENCE=AGGAGCTGGTGCAGGTGCAAGAGATTGAGATGAACAACCACGTAAACAACCAGCCATTGATGATGCCATAGCTTGAGCCATAGTAGTTTCTTTCTTTCTTGAACTTGAATGTGATGATGAT
TARGET=87,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02513 Ps_contig_mira-and-tgicl-ass_franssen_21943
SEQUENCE=CGGGCGGTGATTGTGGAAGCCGGTGAAGTGTTCGGATCGCGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAAWTG
TARGET=39,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02514 Ps_contig_mira-and-tgicl-ass_franssen_21944
SEQUENCE=TAGATAAGTTAATTTAAATTTAAAACAAAAACCAACCYMYAWAMGAMCRCCMAARCMCAAACCAACMMAACCAATACGATCATATTCAATGAGAAGTAGTCTCATCCTTGTGATGATTCTTTGTTCTTMACCATCAGTAGCAGTCTTAGCMTGATAACCAGGAAGCTTCTCTTTGATCTTTTCCAAAATACCTTTCTTCTCTCCCTCATGAATAGTTGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTTGTTACTTCCTCTGTTTTCTTGTGTCCTCCGGGAGCTTCTCCTTAATTTGTCAAGGAACCCTTCTTTCCTCGGTACCGGTTGTTCCTTCAACAACCTCAACTTTCTCAACTGGCACCGAGTNTCTTCTTTCTTCTTCTTCAACTTGGTTCCGACGGTNCTCATAA
TARGET=402,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02515 Ps_contig_mira-and-tgicl-ass_franssen_21951
SEQUENCE=CTATTTGACTTTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGATTGTAACATAGACCCCAGGCCGTAGGTCCACCAGTTGTTCTTTACTTCCATAACCACAAGAGGTTTTGGATCCAACGTGTCCAAGAAAAGCAGCAATCTCCATCATCTGCATAGTTTTATTCCCGGTAGTCCCAAACCCCTGCGGCTCAAAAAGCGCAGCCGCATTTATAAACGAATGATAATCCCAAAACCCACGGCATGAGCTACCGGAGTTGTTTTCTCCTWCGAAAACAGATTCTCGAAATGATTAGGTTTAGAAAATAATCGMYTTATCGTACAAATYACAACAMTAAATCGACCATTGTTACATTCCCNGCCTTGCGTACAGTTACCTTTTTTTCCCGCGTTTTGTTATTTTCCACTTTGTTAACGTTTT
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02516 Ps_contig_mira-and-tgicl-ass_franssen_21951
SEQUENCE=CTATTTGACTTTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGATTGTAACATAGACCCCAGGCCGTAGGTCCACCAGTTGTTCTTTACTTCCATAACCACAAGAGGTTTTGGATCCAACGTGTCCAAGAAAAGCAGCAATCTCCATCATCTGCATAGTTTTATTCCCGGTAGTCCCAAACCCCTGCGGCTCAAAAAGCGCAGCCGCATTTATAAACGAATGATAATCCCAAAACCCACGGCATGAGCTACCGGAGTTGTTTTCTCCTWCGAAAACAGATTCTCGAAATGATTAGGTTTAGAAAATAATCGMYTTATCGTACAAATYACAACAMTAAATCGACCATTGTTACATTCCCNGCCTTGCGTACAGTTACCTTTTTTTCCCGCGTTTTGTTATTTTCCACTTTGTTAACGTTTT
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02517 Ps_contig_mira-and-tgicl-ass_franssen_21967
SEQUENCE=CGCAGAGTACGCGGGGACCATTGATAAGGATACAATTATCAAAAGCGTAACAATCTTTTCAATTTCATTGCAATATAATACACGATGGCCGCATCATCATCATCATCCATGGCTCTCTCTTCTCCAACCTTGGCTGGCAAGCAACTCAAGCTGAACCCATCAAGCCAAAGTAAAGTTT
TARGET=92,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4346
PRIMER_LEFT_PENALTY=0.090580
PRIMER_RIGHT_PENALTY=0.343977
PRIMER_LEFT_SEQUENCE=GGGGACCATTGATAAGGATACA
PRIMER_RIGHT_SEQUENCE=TTTACTTTGGCTTGATGGGTTC
PRIMER_LEFT=12,22
PRIMER_RIGHT=173,22
PRIMER_LEFT_TM=59.909
PRIMER_RIGHT_TM=60.344
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=162
PRIMER_PAIR_PENALTY_1=0.5511
PRIMER_LEFT_1_PENALTY=0.090580
PRIMER_RIGHT_1_PENALTY=0.460533
PRIMER_LEFT_1_SEQUENCE=GGGGACCATTGATAAGGATACA
PRIMER_RIGHT_1_SEQUENCE=AACTTTACTTTGGCTTGATGGG
PRIMER_LEFT_1=12,22
PRIMER_RIGHT_1=176,22
PRIMER_LEFT_1_TM=59.909
PRIMER_RIGHT_1_TM=59.539
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=0.5511
PRIMER_LEFT_2_PENALTY=0.090580
PRIMER_RIGHT_2_PENALTY=0.460533
PRIMER_LEFT_2_SEQUENCE=GGGGACCATTGATAAGGATACA
PRIMER_RIGHT_2_SEQUENCE=CTTTACTTTGGCTTGATGGGTT
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=174,22
PRIMER_LEFT_2_TM=59.909
PRIMER_RIGHT_2_TM=59.539
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=0.7513
PRIMER_LEFT_3_PENALTY=0.090580
PRIMER_RIGHT_3_PENALTY=0.660713
PRIMER_LEFT_3_SEQUENCE=GGGGACCATTGATAAGGATACA
PRIMER_RIGHT_3_SEQUENCE=CTTGATGGGTTCAGCTTGAGTT
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=163,22
PRIMER_LEFT_3_TM=59.909
PRIMER_RIGHT_3_TM=60.661
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=0.8442
PRIMER_LEFT_4_PENALTY=0.090580
PRIMER_RIGHT_4_PENALTY=0.753664
PRIMER_LEFT_4_SEQUENCE=GGGGACCATTGATAAGGATACA
PRIMER_RIGHT_4_SEQUENCE=AGGTTGGAGAAGAGAGAGCCAT
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=129,22
PRIMER_LEFT_4_TM=59.909
PRIMER_RIGHT_4_TM=60.754
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_02518 Ps_contig_mira-and-tgicl-ass_franssen_21989
SEQUENCE=TTAGTAAACAATAAACTAATTAAATTAAATTAAGGTACTTTAATTTTTAAGTTAGTAAATTAACCCCTAAAATTAACTTTAGTAATCAGATTCAGACCTCTTTGTACTTCAACAGGAGCAGCAGTAGGTGCAGTTTCTTTGACTTGATCTTTGTTTTCTTCCTTCACATCCTTAACTCCCTCTTCAATTTCAAAAGCTGACGCAGGGAAAGTCCTGAAAAGCCCTGTAACAGCCCTGAAGGTGATTTTGCCGGTAGGTGGTTCATCCATAAYGATTTCATGAAGAGGAAGCCACATCAAGAGCTCCTTCACCTTCACACCAGTTAGCTTCTTGATCTTTCCAACCTCAACAGTGGCAGTGRCTTCGGTACTATAAATCACATGCCTATCAACTTTCACTAAATTTGTGGTTATAGCTAGTCTTTCTGCTTAAGCCACAACAACCCTCTTTCCCTATGGTACTCCACTTA
TARGET=18,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02519 Ps_contig_mira-and-tgicl-ass_franssen_22019
SEQUENCE=ACGAACGAGAATGAGNGATGCGTAGGGTCGAGCNCCGGCCTCGAACCANGAACTCCACTCCANGCNGACCAACAAAGAATTCCTAAGAACNGCAGCCATTGCCCCACTCTTCCCATGAATGAGCTCAGCTTCTCTGTACCATTTGAGAAATGCTGGGTCCTTGCCAAGGCCTAGTGGGTCGAAACCAAAGTCACCCGGTAGCGATCCGTACGAAGACCAATTACGGGGTACGACGAGGTTTCCACCGAAACTAACACCAGGGATCCATGATTTCTTAGGTGCAGAAGCAGCAGCAGCAACAATAAGTCTTTTAGGACTAACAGCTGCAACACCATTGCTTCCAATGGCAGTAGCTAGCGTAAGGTTTGT
TARGET=286,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0451
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.041548
PRIMER_LEFT_SEQUENCE=TGTACCATTTGAGAAATGCTGG
PRIMER_RIGHT_SEQUENCE=CCTTACGCTAGCTACTGCCATT
PRIMER_LEFT=134,22
PRIMER_RIGHT=363,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.958
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.0747
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.041548
PRIMER_LEFT_1_SEQUENCE=CTAGTGGGTCGAAACCAAAGTC
PRIMER_RIGHT_1_SEQUENCE=CCTTACGCTAGCTACTGCCATT
PRIMER_LEFT_1=170,22
PRIMER_RIGHT_1=363,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=59.958
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=194
PRIMER_PAIR_PENALTY_2=0.2358
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.232246
PRIMER_LEFT_2_SEQUENCE=TGTACCATTTGAGAAATGCTGG
PRIMER_RIGHT_2_SEQUENCE=CAATGGTGTTGCAGCTGTTAGT
PRIMER_LEFT_2=134,22
PRIMER_RIGHT_2=336,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=60.232
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.2370
PRIMER_LEFT_3_PENALTY=0.195430
PRIMER_RIGHT_3_PENALTY=0.041548
PRIMER_LEFT_3_SEQUENCE=GGATCCATGATTTCTTAGGTGC
PRIMER_RIGHT_3_SEQUENCE=CCTTACGCTAGCTACTGCCATT
PRIMER_LEFT_3=260,22
PRIMER_RIGHT_3=363,22
PRIMER_LEFT_3_TM=59.805
PRIMER_RIGHT_3_TM=59.958
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=104
PRIMER_PAIR_PENALTY_4=0.2487
PRIMER_LEFT_4_PENALTY=0.207104
PRIMER_RIGHT_4_PENALTY=0.041548
PRIMER_LEFT_4_SEQUENCE=CAGGGATCCATGATTTCTTAGG
PRIMER_RIGHT_4_SEQUENCE=CCTTACGCTAGCTACTGCCATT
PRIMER_LEFT_4=257,22
PRIMER_RIGHT_4=363,22
PRIMER_LEFT_4_TM=59.793
PRIMER_RIGHT_4_TM=59.958
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=107
=
PRIMER_SEQUENCE_ID=SSR_02520 Ps_contig_mira-and-tgicl-ass_franssen_22020
SEQUENCE=CGGGGGTTTTTTTACCTTTTTAATTTATCAGCATCATCATCATCATCACATTCAAGTTCRAGAAAGAAAGAAACTACTATGGCTCAAGCTATGGCATCATCAATGGCTGGTTGTTTACGTGGTTGTTCATCTCAACTGTGTTAGAAGGATCTTNAATTGNGGACCAANGGTTGAGTTTGTG
TARGET=32,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02521 Ps_contig_mira-and-tgicl-ass_franssen_22020
SEQUENCE=CGGGGGTTTTTTTACCTTTTTAATTTATCAGCATCATCATCATCATCACATTCAAGTTCRAGAAAGAAAGAAACTACTATGGCTCAAGCTATGGCATCATCAATGGCTGGTTGTTTACGTGGTTGTTCATCTCAACTGTGTTAGAAGGATCTTNAATTGNGGACCAANGGTTGAGTTTGTG
TARGET=61,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02522 Ps_contig_mira-and-tgicl-ass_franssen_22028
SEQUENCE=ATGGTGGTGGAGGTTACGGTCNTGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACARCGGTGGCGGTGTTTCCAACAATGAGGTTGTTGARAAGTCAAATGAAGTAAATGATGCCAAACATGGCGGTGGACACTATGGTGGTGGACACTATGGAGGTGGACACTATGGTAGGTAGGTACAACTAACGGTCATGGTGGTGGTCATCATGGTGGACATGGTGGTGCTTCCGACAATGGTAACTGATGATACCATGATAAATGATGCACATTCAAAGTAATAAGTTTCATGAATG
TARGET=73,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02523 Ps_contig_mira-and-tgicl-ass_franssen_22028
SEQUENCE=ATGGTGGTGGAGGTTACGGTCNTGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACARCGGTGGCGGTGTTTCCAACAATGAGGTTGTTGARAAGTCAAATGAAGTAAATGATGCCAAACATGGCGGTGGACACTATGGTGGTGGACACTATGGAGGTGGACACTATGGTAGGTAGGTACAACTAACGGTCATGGTGGTGGTCATCATGGTGGACATGGTGGTGCTTCCGACAATGGTAACTGATGATACCATGATAAATGATGCACATTCAAAGTAATAAGTTTCATGAATG
TARGET=223,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02524 Ps_contig_mira-and-tgicl-ass_franssen_22051
SEQUENCE=ACTGTTCAGGAGATGCTAAGTTTAGCGACCAAGTACAACAAGGCAGTGCAAGAGGAAGATGAGCTGCCCCCAGAAAAGCTTGCAATTGCTAATGTGGGAAGGTCTAGGATGCTAAGAAGCATCTTGAAGAGCACGTTTCTAATTTAATGTCTTCGAACATTGTTCAGACTTTGGGAATGATGCTCGACACAGTTGTGTTCTAGAGTTAGGATCGTCCACTGTATTCTTCTTCTTCTTCATGATGTCCAGCAATATTTCATTTTACACTTCTTTGACCTTGTTTGCAATAGTATTTGTTTTTTATTAACTAAAGTTTATTTGCTATTTCATTGTAYGTTCGTCTACTAAATTCGGTTTCCGGATGATAATCTATTTCTAAGTC
TARGET=224,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02525 Ps_contig_mira-and-tgicl-ass_franssen_22061
SEQUENCE=TGTTTTGGTTAAGTTTGTTAAAGGTTATTTTGGTTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGYGTTGTATGGATTAGGTGTTGAGACAGACTAATGAAAGAAAGAAAGTNTAATTGGTNAGTATTACAGTATCTCTTATGGTTGTTGT
TARGET=168,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02526 Ps_contig_mira-and-tgicl-ass_franssen_22064
SEQUENCE=GTCGGGCAAGCAAACAACTACATCTATTCATCTCCACCACCACCGGTGCACTCACCTCCCCCACCTTATTCATTTATAGTTCACCACCACCACCATACACTCACCTCCTCCACCTTATCGATTACAATTCACCACCACCACCGTCAGAGAAGACCATACAAATATCCTTACTCCTTCCACCACCAGTTTACAAATACAAGTCACCACCTCCACCGGTATACAATTCACYMYCACMTACCGGTTAAAAACCA
TARGET=82,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02527 Ps_contig_mira-and-tgicl-ass_franssen_22064
SEQUENCE=GTCGGGCAAGCAAACAACTACATCTATTCATCTCCACCACCACCGGTGCACTCACCTCCCCCACCTTATTCATTTATAGTTCACCACCACCACCATACACTCACCTCCTCCACCTTATCGATTACAATTCACCACCACCACCGTCAGAGAAGACCATACAAATATCCTTACTCCTTCCACCACCAGTTTACAAATACAAGTCACCACCTCCACCGGTATACAATTCACYMYCACMTACCGGTTAAAAACCA
TARGET=130,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02528 Ps_contig_mira-and-tgicl-ass_franssen_22078
SEQUENCE=GTCGGGGACTAATCATTTTTCTCTACGGCAATAGAGATCGGAAATTCGAACTTCTTTCTCTCTCTCTCTCTGAAAAATCGTAAAACCGTTTATCTTAGATCGGATCTATTCGACGTCGTTTCGGTAGGAATGGCTCCATTCAGCGGCGATGAAACGGCACCGTTTTTGGCTTCCTTGGAGCCGCAGCTGCTCTCGTCTTCTCATGTATGGGANCTGCGTATGGTACTGAACAAAAAGTGGTGGG
TARGET=57,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1764
PRIMER_LEFT_PENALTY=0.044097
PRIMER_RIGHT_PENALTY=0.132262
PRIMER_LEFT_SEQUENCE=TACGGCAATAGAGATCGGAAAT
PRIMER_RIGHT_SEQUENCE=GNTCCCATACATGAGAAGACGA
PRIMER_LEFT=23,22
PRIMER_RIGHT=213,22
PRIMER_LEFT_TM=59.956
PRIMER_RIGHT_TM=60.132
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.2578
PRIMER_LEFT_1_PENALTY=0.044097
PRIMER_RIGHT_1_PENALTY=0.213725
PRIMER_LEFT_1_SEQUENCE=TACGGCAATAGAGATCGGAAAT
PRIMER_RIGHT_1_SEQUENCE=TACATGAGAAGACGAGAGCAGC
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=206,22
PRIMER_LEFT_1_TM=59.956
PRIMER_RIGHT_1_TM=59.786
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.3144
PRIMER_LEFT_2_PENALTY=0.044097
PRIMER_RIGHT_2_PENALTY=0.270338
PRIMER_LEFT_2_SEQUENCE=TACGGCAATAGAGATCGGAAAT
PRIMER_RIGHT_2_SEQUENCE=GCAGNTCCCATACATGAGAAGA
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=216,22
PRIMER_LEFT_2_TM=59.956
PRIMER_RIGHT_2_TM=60.270
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.3452
PRIMER_LEFT_3_PENALTY=0.044097
PRIMER_RIGHT_3_PENALTY=0.301100
PRIMER_LEFT_3_SEQUENCE=TACGGCAATAGAGATCGGAAAT
PRIMER_RIGHT_3_SEQUENCE=GTTCAGTACCATACGCAGNTCC
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=230,22
PRIMER_LEFT_3_TM=59.956
PRIMER_RIGHT_3_TM=59.699
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.5500
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.3502
PRIMER_LEFT_4_PENALTY=0.044097
PRIMER_RIGHT_4_PENALTY=0.306150
PRIMER_LEFT_4_SEQUENCE=TACGGCAATAGAGATCGGAAAT
PRIMER_RIGHT_4_SEQUENCE=TCCCATACATGAGAAGACGAGA
PRIMER_LEFT_4=23,22
PRIMER_RIGHT_4=211,22
PRIMER_LEFT_4_TM=59.956
PRIMER_RIGHT_4_TM=59.694
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_02529 Ps_contig_mira-and-tgicl-ass_franssen_22085
SEQUENCE=AAATAAAAGTAGTTCATGGAGATTCAAGAACTTAGATGCTTTTTAATTTTTTTAAATTTGGTAACTTTTGTTTTTTTATTTTATTTTAATTATTAGTAGTATGGTAGCTCAATGTGGAGCATGTGGATGATGTGATGTATGGTATTTCATGGTGGTGCATGATGGCATGTAGTNACTAATTGTTTATGTATTATTTGTAACAAACT
TARGET=74,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.8620
PRIMER_LEFT_PENALTY=3.485290
PRIMER_RIGHT_PENALTY=0.376709
PRIMER_LEFT_SEQUENCE=CATGGAGATTCAAGAACTTAGATGC
PRIMER_RIGHT_SEQUENCE=GTNACTACATGCCATCATGCAC
PRIMER_LEFT=14,25
PRIMER_RIGHT=175,22
PRIMER_LEFT_TM=60.485
PRIMER_RIGHT_TM=59.623
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=162
PRIMER_PAIR_PENALTY_1=3.9058
PRIMER_LEFT_1_PENALTY=3.485290
PRIMER_RIGHT_1_PENALTY=0.420521
PRIMER_LEFT_1_SEQUENCE=CATGGAGATTCAAGAACTTAGATGC
PRIMER_RIGHT_1_SEQUENCE=ATCATGCACCACCATGAAATAC
PRIMER_LEFT_1=14,25
PRIMER_RIGHT_1=162,22
PRIMER_LEFT_1_TM=60.485
PRIMER_RIGHT_1_TM=59.579
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=4.1430
PRIMER_LEFT_2_PENALTY=3.485290
PRIMER_RIGHT_2_PENALTY=0.657742
PRIMER_LEFT_2_SEQUENCE=CATGGAGATTCAAGAACTTAGATGC
PRIMER_RIGHT_2_SEQUENCE=TGCTCCACATTGAGCTACCATA
PRIMER_LEFT_2=14,25
PRIMER_RIGHT_2=120,22
PRIMER_LEFT_2_TM=60.485
PRIMER_RIGHT_2_TM=60.658
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=107
PRIMER_PAIR_PENALTY_3=4.2049
PRIMER_LEFT_3_PENALTY=3.485290
PRIMER_RIGHT_3_PENALTY=0.719637
PRIMER_LEFT_3_SEQUENCE=CATGGAGATTCAAGAACTTAGATGC
PRIMER_RIGHT_3_SEQUENCE=CACATGCTCCACATTGAGCTAC
PRIMER_LEFT_3=14,25
PRIMER_RIGHT_3=124,22
PRIMER_LEFT_3_TM=60.485
PRIMER_RIGHT_3_TM=60.720
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=111
PRIMER_PAIR_PENALTY_4=4.3080
PRIMER_LEFT_4_PENALTY=3.485290
PRIMER_RIGHT_4_PENALTY=0.822662
PRIMER_LEFT_4_SEQUENCE=CATGGAGATTCAAGAACTTAGATGC
PRIMER_RIGHT_4_SEQUENCE=ACATCACATCATCCACATGCTC
PRIMER_LEFT_4=14,25
PRIMER_RIGHT_4=137,22
PRIMER_LEFT_4_TM=60.485
PRIMER_RIGHT_4_TM=60.823
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_02530 Ps_contig_mira-and-tgicl-ass_franssen_22109
SEQUENCE=ACNTACATGATTGCNGTTTCANGTTCACTGGNACATTCCGATNACGTACCGGNTTAACCGGCAAGAAGTTCGGATCCGCTGTGATCTTAAACCAGGGGAAAATTTGGGATGATCAGTGGGGTTTTCTGGGTTGGTACCAATAATTGGAGCAACCGTGGCTGCAATTTACCACCAATACATTCTAAGAGGATCAGCAATTAAAGCTCTTGGAATCATTCAGGAGCAACGCTTAAYTGATTAATTAATTAAGCTAGCTAAATTGACTTAATTACCGAAAGGAAGATTAAGTTGGGTTAGTTTAAACTTTCCCAACAACAAAG
TARGET=237,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02531 Ps_contig_mira-and-tgicl-ass_franssen_22125
SEQUENCE=CGGGTACTTTCTTTTATTTTCTTSAGTTGAAGCAGCGAGCCAGAAACCTAGGGAGAGAGGAAGAGGACTGCGCCTCGTTTCACCACCATCATTCAACATGGGTTATTTCCAGGGATTCTATGCACAAGAGGCGTGAGACTGGAGGAAAGAAGAAGGCAGTGGAAGGAAGAAGAAGAAAGTACGAGCGTTGGTCCGCCACGCCAGACCTAACCACCAAGTCTTACAAGACAAACAACGGAACCGGTAAGGGAGACTCCGTGTCCGTGGTGTAA
TARGET=166,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02532 Ps_contig_mira-and-tgicl-ass_franssen_22130
SEQUENCE=CTTCTCCTTCCTACCGGTCCGTACTCTACCGACCACCTTCCTTTCTACCTTCGGATTGGTCGGAGTTCTATTTGTCTCTTTTCTTACGTCGTTTCCGGTTCCGTCCTTTGGTCTCCTCCTCCTCCGACCCGTCCACCACCAAACCCGCCCACCATCCAAGCAGAGGTTCCCCTCTCCGACCCCCGTTCCAGCTAGGTGAAATCACCGAAGAAGAGCTCAAGGCCTACGATGGCAACGATCACGATAAGCCCYTGCTCATGGCCATTAAGGGCCAGATCTATGATGTCTCCCAGAGCAGGATGTTCTAGTCGGGACCCCGGCGGTTCCTTATGTCTTTATTGCTGGACAAGGNTGCTAGTAGAGTCTTAGACAAAGTNTTGTT
TARGET=113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02533 Ps_contig_mira-and-tgicl-ass_franssen_22132
SEQUENCE=TGGTATCAACGCAGAGTTGTTGGAAGTTGTTAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATYTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTTGTTGAGACAGACTTAATTGAAAGAAAGAAAGTATAATTGGGTTGTAAGTATTACAGTATACTCTTATGTGTTGTGTATTTCTTTTTTACTTGTGTACTCTTCTACCAGTAAAG
TARGET=179,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02534 Ps_contig_mira-and-tgicl-ass_franssen_22143
SEQUENCE=ACGTGGGGACACAAAAAAAACACACCAAGCCRGTGAAGCTAGCTARCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATTGTTCAACTATCCACTTCGACCCTCTWAACMAAAGAAGTRKTTTCTAGCTTGTTACAAAACCTATACGTAACCAAAAACAACTACCCCATCTACAAAAGAACAAACTTACG
TARGET=66,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02535 Ps_contig_mira-and-tgicl-ass_franssen_22147
SEQUENCE=TTTACCCCCCGTACCCCGTACCCGTTACCCCGTAACCCCTAAAACCCCCGTAAACCCCGGGTAACCCGGGTAACCCGGTACCGGTCCCGTTCCCTTTCCCGTTCCGTCCCGTCCCTTCCCTCCCCGTCCCACCCTACCTACCTAACCCTACCCCTACCCGAAAACCGGTAAAGGGTTACCCGGGTACCCCGGTCCCGTTTCCCTTACCCCTAAACCCCGAAACCGTTTAACCTTTCCCTAACCCCCTTTACCTTCCCCTTCCCGGTCCGACGCATTTCCTTTTCCTTACGTCAAACCCAACTTGTTGTCTGCAACTGCTCTCTCTGCTGCTTTCTTTGCCATCGTTTTCTAGGACTCGTCCACGTGGATCTTGATCTGACTTTTCATTGACGGACGGACGGGATCTGTTTGATTTAAATTCTGAGTAGCGTTTTTATTTTGTTTTCATGTGTCTATTTATACTTTTTCGTCATGTGTTTGTTGATTCGACGGACGGGATCGATCTTCATGATTANGATGATGATTTTATTTGNGATTCTATTTTGCTTTGGTNTCACG
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2057
PRIMER_LEFT_PENALTY=0.112603
PRIMER_RIGHT_PENALTY=0.093130
PRIMER_LEFT_SEQUENCE=GTTACCCCGTAACCCCTAAAAC
PRIMER_RIGHT_SEQUENCE=AAGTTGGGTTTGACGTAAGGAA
PRIMER_LEFT=23,22
PRIMER_RIGHT=302,22
PRIMER_LEFT_TM=59.887
PRIMER_RIGHT_TM=59.907
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.2057
PRIMER_LEFT_1_PENALTY=0.112603
PRIMER_RIGHT_1_PENALTY=0.093130
PRIMER_LEFT_1_SEQUENCE=GTTACCCCGTAACCCCTAAAAC
PRIMER_RIGHT_1_SEQUENCE=AGTTGGGTTTGACGTAAGGAAA
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=301,22
PRIMER_LEFT_1_TM=59.887
PRIMER_RIGHT_1_TM=59.907
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.3499
PRIMER_LEFT_2_PENALTY=0.112603
PRIMER_RIGHT_2_PENALTY=0.237277
PRIMER_LEFT_2_SEQUENCE=GTTACCCCGTAACCCCTAAAAC
PRIMER_RIGHT_2_SEQUENCE=GAGAGCAGTTGCAGACAACAAG
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=321,22
PRIMER_LEFT_2_TM=59.887
PRIMER_RIGHT_2_TM=60.237
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=0.3499
PRIMER_LEFT_3_PENALTY=0.112603
PRIMER_RIGHT_3_PENALTY=0.237277
PRIMER_LEFT_3_SEQUENCE=GTTACCCCGTAACCCCTAAAAC
PRIMER_RIGHT_3_SEQUENCE=AGAGAGCAGTTGCAGACAACAA
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=322,22
PRIMER_LEFT_3_TM=59.887
PRIMER_RIGHT_3_TM=60.237
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.4200
PRIMER_LEFT_4_PENALTY=0.112603
PRIMER_RIGHT_4_PENALTY=0.307443
PRIMER_LEFT_4_SEQUENCE=GTTACCCCGTAACCCCTAAAAC
PRIMER_RIGHT_4_SEQUENCE=ACAAGTTGGGTTTGACGTAAGG
PRIMER_LEFT_4=23,22
PRIMER_RIGHT_4=304,22
PRIMER_LEFT_4_TM=59.887
PRIMER_RIGHT_4_TM=60.307
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_02536 Ps_contig_mira-and-tgicl-ass_franssen_22147
SEQUENCE=TTTACCCCCCGTACCCCGTACCCGTTACCCCGTAACCCCTAAAACCCCCGTAAACCCCGGGTAACCCGGGTAACCCGGTACCGGTCCCGTTCCCTTTCCCGTTCCGTCCCGTCCCTTCCCTCCCCGTCCCACCCTACCTACCTAACCCTACCCCTACCCGAAAACCGGTAAAGGGTTACCCGGGTACCCCGGTCCCGTTTCCCTTACCCCTAAACCCCGAAACCGTTTAACCTTTCCCTAACCCCCTTTACCTTCCCCTTCCCGGTCCGACGCATTTCCTTTTCCTTACGTCAAACCCAACTTGTTGTCTGCAACTGCTCTCTCTGCTGCTTTCTTTGCCATCGTTTTCTAGGACTCGTCCACGTGGATCTTGATCTGACTTTTCATTGACGGACGGACGGGATCTGTTTGATTTAAATTCTGAGTAGCGTTTTTATTTTGTTTTCATGTGTCTATTTATACTTTTTCGTCATGTGTTTGTTGATTCGACGGACGGGATCGATCTTCATGATTANGATGATGATTTTATTTGNGATTCTATTTTGCTTTGGTNTCACG
TARGET=389,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7709
PRIMER_LEFT_PENALTY=0.093130
PRIMER_RIGHT_PENALTY=0.677801
PRIMER_LEFT_SEQUENCE=TTTCCTTACGTCAAACCCAACT
PRIMER_RIGHT_SEQUENCE=NTAATCATGAAGATCGATCCCG
PRIMER_LEFT=280,22
PRIMER_RIGHT=514,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.678
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=0.7709
PRIMER_LEFT_1_PENALTY=0.093130
PRIMER_RIGHT_1_PENALTY=0.677801
PRIMER_LEFT_1_SEQUENCE=TTCCTTACGTCAAACCCAACTT
PRIMER_RIGHT_1_SEQUENCE=NTAATCATGAAGATCGATCCCG
PRIMER_LEFT_1=281,22
PRIMER_RIGHT_1=514,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=60.678
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.7806
PRIMER_LEFT_2_PENALTY=0.102789
PRIMER_RIGHT_2_PENALTY=0.677801
PRIMER_LEFT_2_SEQUENCE=ACTGCTCTCTCTGCTGCTTTCT
PRIMER_RIGHT_2_SEQUENCE=NTAATCATGAAGATCGATCCCG
PRIMER_LEFT_2=313,22
PRIMER_RIGHT_2=514,22
PRIMER_LEFT_2_TM=60.103
PRIMER_RIGHT_2_TM=60.678
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.8069
PRIMER_LEFT_3_PENALTY=0.129103
PRIMER_RIGHT_3_PENALTY=0.677801
PRIMER_LEFT_3_SEQUENCE=CTTTGCCATCGTTTTCTAGGAC
PRIMER_RIGHT_3_SEQUENCE=NTAATCATGAAGATCGATCCCG
PRIMER_LEFT_3=333,22
PRIMER_RIGHT_3=514,22
PRIMER_LEFT_3_TM=60.129
PRIMER_RIGHT_3_TM=60.678
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=0.8069
PRIMER_LEFT_4_PENALTY=0.129103
PRIMER_RIGHT_4_PENALTY=0.677801
PRIMER_LEFT_4_SEQUENCE=TTTGCCATCGTTTTCTAGGACT
PRIMER_RIGHT_4_SEQUENCE=NTAATCATGAAGATCGATCCCG
PRIMER_LEFT_4=334,22
PRIMER_RIGHT_4=514,22
PRIMER_LEFT_4_TM=60.129
PRIMER_RIGHT_4_TM=60.678
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_02537 Ps_contig_mira-and-tgicl-ass_franssen_22166
SEQUENCE=AAAAAAGAAAACAAAAGTAAGGTAATACAACGCAGAGTACGTCGGGGWSRRSWACAACAACAAGAAGAACAAGGGTTTTTCACTTCCTCGTTTAAATCGAAAACATTAACCAAAACATGGGCAGAAAGTTCTTCGTCGGCGGTAACTGGAAATGCAATGGAACCACTGAAGAGGTGAAAGAAAATTGTGGGTACTTTGAACGAAGCTAAACGTCCCGGAGAAGATGTTGTTGAAGTTGTTGTGACCGTCCCCCCTTACGTTCCTTTCCTTTCCTTTCGTAGAAAACGTCTTCGTCCTT
TARGET=260,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02538 Ps_contig_mira-and-tgicl-ass_franssen_22172
SEQUENCE=AAGTTCNTAAAGGCAAGCNGCATGAAGGTGAAGACTGGCATGACAACTGATACAGTTAAACACTGGACTTATGTCATAGGTGGTGAGGTACACACATGTTATGAGAGTTTTGAAGAAGTTGATGAACAAAATAAAAAACTGACTCTCAAGCTTTTTGGTGGAGACATTGAGAAGTTTCACTATAAGCTCTTTAAAGTCACCCTTGAAGTAGTTGATAAGGCTGWATYGGCACTGCTGTTGCTAAATGGACTGTTGAATATGAGAAAATCAGTGAGGATATTCATCCTCCAAATGGATGGATGGACTATCTTAGCCAATGCCACTAGAGATATTGATGGTCATTCTTATAACAAAAATTATCATTAAGGCACTATATGATAGAAATAAAGATTAGTTTAAATTAAAGTTGGGCGTAGTA
TARGET=292,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02539 Ps_contig_mira-and-tgicl-ass_franssen_22176
SEQUENCE=CTATACTTTGTTTGGTGTGGTTTATTTTCTTCAATCGGGAATAAAGAGATTAGGGTTTCACGTAGAACATGGTCTCTTCTCTCATGTGAATCAGCTCAATTGTTTTGTTGTTGGTGCGCAGTGTGTGTGTGTTGTAAATTGCGAACTACAGAATTTGATTCCTACTAAATAAAAAACTCAAATCTATTTTGCAGATAATAAMWAACT
TARGET=121,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02540 Ps_contig_mira-and-tgicl-ass_franssen_22185
SEQUENCE=GGTTTAAAGCATATTCTTCGGTTTATCTATAATATAAAGGGTGAACAACAAACACTTCAGAACAACAACAACAAGAAGAACAAGGGTTTTTCACTTCCTCGTTTAAATCGAAAACATTAACCAAAACATGGGCAGAAAGTTCTTCGTCGGCGGTAACTGGAAATGCAATGGAACCACTGAAGAGGTGAAGAAAATTGTGGGTTACTTTGAACGAAGCTAAAGTCCCCGGAGAAGATGTTGTTGAAGTTGTTGTGACGCCCCCCTTACGTCTTTCCTTCCTTTGTAGAAAAGCTTGCTTCGCTCT
TARGET=61,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4632
PRIMER_LEFT_PENALTY=0.461740
PRIMER_RIGHT_PENALTY=0.001455
PRIMER_LEFT_SEQUENCE=AGGGTGAACAACAAACACTTCA
PRIMER_RIGHT_SEQUENCE=AAAGGAAGGAAAGACGTAAGGG
PRIMER_LEFT=37,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=59.538
PRIMER_RIGHT_TM=59.999
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=245
PRIMER_PAIR_PENALTY_1=0.4632
PRIMER_LEFT_1_PENALTY=0.461740
PRIMER_RIGHT_1_PENALTY=0.001455
PRIMER_LEFT_1_SEQUENCE=GGGTGAACAACAAACACTTCAG
PRIMER_RIGHT_1_SEQUENCE=AAAGGAAGGAAAGACGTAAGGG
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=281,22
PRIMER_LEFT_1_TM=59.538
PRIMER_RIGHT_1_TM=59.999
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.7617
PRIMER_LEFT_2_PENALTY=0.461740
PRIMER_RIGHT_2_PENALTY=0.299936
PRIMER_LEFT_2_SEQUENCE=AGGGTGAACAACAAACACTTCA
PRIMER_RIGHT_2_SEQUENCE=CGTCACAACAACTTCAACAACA
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=256,22
PRIMER_LEFT_2_TM=59.538
PRIMER_RIGHT_2_TM=59.700
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.7617
PRIMER_LEFT_3_PENALTY=0.461740
PRIMER_RIGHT_3_PENALTY=0.299936
PRIMER_LEFT_3_SEQUENCE=GGGTGAACAACAAACACTTCAG
PRIMER_RIGHT_3_SEQUENCE=CGTCACAACAACTTCAACAACA
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=256,22
PRIMER_LEFT_3_TM=59.538
PRIMER_RIGHT_3_TM=59.700
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=219
PRIMER_PAIR_PENALTY_4=0.7662
PRIMER_LEFT_4_PENALTY=0.461740
PRIMER_RIGHT_4_PENALTY=0.304436
PRIMER_LEFT_4_SEQUENCE=AGGGTGAACAACAAACACTTCA
PRIMER_RIGHT_4_SEQUENCE=AGCTTCGTTCAAAGTAACCCAC
PRIMER_LEFT_4=37,22
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=59.538
PRIMER_RIGHT_4_TM=59.696
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_02541 Ps_contig_mira-and-tgicl-ass_franssen_22192
SEQUENCE=TCCACATNTTAATTCATGANATTTATTTACTTGAGGAAATGTTGATATTATCAGTACCGTGTTGGAAGCACCACCATGTCCACCGTGTCCACCTCCACCATTGGTAACCACCACCACCATTATGGTAACCTCCACCGCCATTGTAACCACCTCCACCATTATGGTAACCACCTCCATTATGGTAACCACCACCACGTCCATAACCTCCATATTTAGCATTCGTTTACTTCATNTGTCTTTCAACAACCTCCTNAGTTTAACTTCCCTAG
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5888
PRIMER_LEFT_PENALTY=0.554687
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=TATCAGTACCGTGTTGGAAGCA
PRIMER_RIGHT_SEQUENCE=AATATGGAGGTTATGGACGTGG
PRIMER_LEFT=48,22
PRIMER_RIGHT=212,22
PRIMER_LEFT_TM=60.555
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=0.8370
PRIMER_LEFT_1_PENALTY=0.554687
PRIMER_RIGHT_1_PENALTY=0.282356
PRIMER_LEFT_1_SEQUENCE=TATCAGTACCGTGTTGGAAGCA
PRIMER_RIGHT_1_SEQUENCE=ACGTGGTGGTGGTTACCATAAT
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=196,22
PRIMER_LEFT_1_TM=60.555
PRIMER_RIGHT_1_TM=60.282
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=5.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.8765
PRIMER_LEFT_2_PENALTY=0.842379
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=TTATCAGTACCGTGTTGGAAGC
PRIMER_RIGHT_2_SEQUENCE=AATATGGAGGTTATGGACGTGG
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=59.158
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=0.9071
PRIMER_LEFT_3_PENALTY=0.554687
PRIMER_RIGHT_3_PENALTY=0.352395
PRIMER_LEFT_3_SEQUENCE=TATCAGTACCGTGTTGGAAGCA
PRIMER_RIGHT_3_SEQUENCE=GTGGTTACCATAATGGAGGTGG
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=188,22
PRIMER_LEFT_3_TM=60.555
PRIMER_RIGHT_3_TM=60.352
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=141
PRIMER_PAIR_PENALTY_4=0.9071
PRIMER_LEFT_4_PENALTY=0.554687
PRIMER_RIGHT_4_PENALTY=0.352395
PRIMER_LEFT_4_SEQUENCE=TATCAGTACCGTGTTGGAAGCA
PRIMER_RIGHT_4_SEQUENCE=GTGGTGGTTACCATAATGGAGG
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=191,22
PRIMER_LEFT_4_TM=60.555
PRIMER_RIGHT_4_TM=60.352
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_02542 Ps_contig_mira-and-tgicl-ass_franssen_22195
SEQUENCE=CTAGAACGTAATATTTTATTTAATTTGGTGATCCCTTCAAACGAGTAGGTTGCAAGATACATTACACACATGCCTTAGAACTGTCTAAGTTCAAAAACCAAAACAAACTAACAGTGAGAATAGCAAAACACATGAGATAAAAAGAGTGGCTTTTCTTGAAACAGACTACGTTGAAACTTTGCTTCCTTGTATTATTGCCAATGCATCTTCCTGCTTATTTCCAATATATACCTAAAATCCRAGGGATCTACTATAAACCAGTATCTAGTAGTGGTATGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGACTTATATTTGTAAATCGGTGGTGGAGGAGAAGAATATTTATAAGATTTCT
TARGET=298,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02543 Ps_contig_mira-and-tgicl-ass_franssen_22196
SEQUENCE=TAACTGACTTCTCAAGGANACCACAATGATGGAANACAANATACCAGCAGCAGCAGCAACATAGCTCAATCGGACATAAGGCTGACTTCTACAAAGGACACCACAATTGATGGAACACAAACCAAAACCGAATACTATAGCGAGACAGATGTGATTTATCAACCCGGATATGTTGCCAAAAATCAAAGTAACACATGCAACCAAACACGTCACAATCACAATCACGCCGCTACTGGCACCACCATTGTTACCGGCACCACTGCGGAAAAATGCCATGGCAGAAACAGGAGGGAGAGGAGAAACATGTTCCAGAGGATGAAGGATGGCATATCAGGTCACAGCAGTGACAGTGGCAGTAGCAGTGATGAGTGTGACAGCGACAATGAAAACCGTCGCCACAGGAAGAACTGCAATTGAGGAGCAGCTGTTCCAATAATAATAAATTGGAGTTTGAGGATCGAAGTTGGCAACTCAAAGTCCAATATTATGTGCGTGATGATTGCG
TARGET=45,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5097
PRIMER_LEFT_PENALTY=0.400471
PRIMER_RIGHT_PENALTY=0.109273
PRIMER_LEFT_SEQUENCE=TCTCAAGGANACCACAATGATG
PRIMER_RIGHT_SEQUENCE=GTTTCTCCTCTCCCTCCTGTTT
PRIMER_LEFT=9,22
PRIMER_RIGHT=302,22
PRIMER_LEFT_TM=59.600
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.75
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=0.7330
PRIMER_LEFT_1_PENALTY=0.623739
PRIMER_RIGHT_1_PENALTY=0.109273
PRIMER_LEFT_1_SEQUENCE=TGACTTCTCAAGGANACCACAA
PRIMER_RIGHT_1_SEQUENCE=GTTTCTCCTCTCCCTCCTGTTT
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=302,22
PRIMER_LEFT_1_TM=59.376
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.75
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=299
PRIMER_PAIR_PENALTY_2=0.8912
PRIMER_LEFT_2_PENALTY=0.400471
PRIMER_RIGHT_2_PENALTY=0.490741
PRIMER_LEFT_2_SEQUENCE=TCTCAAGGANACCACAATGATG
PRIMER_RIGHT_2_SEQUENCE=ATGTTTCTCCTCTCCCTCCTGT
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=304,22
PRIMER_LEFT_2_TM=59.600
PRIMER_RIGHT_2_TM=60.491
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.75
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=0.8912
PRIMER_LEFT_3_PENALTY=0.400471
PRIMER_RIGHT_3_PENALTY=0.490741
PRIMER_LEFT_3_SEQUENCE=TCTCAAGGANACCACAATGATG
PRIMER_RIGHT_3_SEQUENCE=ACATGTTTCTCCTCTCCCTCCT
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=306,22
PRIMER_LEFT_3_TM=59.600
PRIMER_RIGHT_3_TM=60.491
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.75
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.9626
PRIMER_LEFT_4_PENALTY=0.400471
PRIMER_RIGHT_4_PENALTY=0.562082
PRIMER_LEFT_4_SEQUENCE=TCTCAAGGANACCACAATGATG
PRIMER_RIGHT_4_SEQUENCE=TCCGGGTTGATAAATCACATCT
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=167,22
PRIMER_LEFT_4_TM=59.600
PRIMER_RIGHT_4_TM=60.562
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_02544 Ps_contig_mira-and-tgicl-ass_franssen_22201
SEQUENCE=CCTAAAATACCAACCGACCCCTAAACTTTTTAAWTTTAAAACTAATTWCTTTTATTTACTATCATAATAATSSCTTAATGATTAATTTTTGTATAAGATGACCATCAATATCTCTAGTGCATTGGCTAAGATAGTCCATCCATCCATTTGGAGGATGAATATCCTCACTGATTTTCTCATATTCAACAGTCCATTTTAGCAACAGCAGTGCCATCAGCCTTATCAACTACTTCAAGGGTGACTTTAAAGAGCTTATAGTGCTTCTCAATGTCTCCAACCAAAAGCTTGAGTAGTCTAGTTTTTTTATTTGTCATCACTTCTACAAACTCNATACNTGTGTGTACC
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02545 Ps_contig_mira-and-tgicl-ass_franssen_22228
SEQUENCE=GTCGGGGAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCATACCATTACNCATCTCCTCCACCTCCACCAGCTCACACCTATCCCTCAA
TARGET=137,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2868
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.261226
PRIMER_LEFT_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_SEQUENCE=GAGGTGGAGGAGATGNGTAATG
PRIMER_LEFT=77,22
PRIMER_RIGHT=204,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=60.261
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.75
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=1.0151
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.989557
PRIMER_LEFT_1_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_1_SEQUENCE=GGAGGTGGAGGAGATGNGTAAT
PRIMER_LEFT_1=77,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=60.990
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=1.1552
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=1.129645
PRIMER_LEFT_2_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_2_SEQUENCE=NGTAATGGTATGGATCCTTTGG
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=189,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=58.870
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=1.2872
PRIMER_LEFT_3_PENALTY=0.025566
PRIMER_RIGHT_3_PENALTY=1.261614
PRIMER_LEFT_3_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_3_SEQUENCE=TTGAGGGATAGGTGTGAGCTG
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=228,21
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=60.262
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=1.2894
PRIMER_LEFT_4_PENALTY=0.025566
PRIMER_RIGHT_4_PENALTY=1.263803
PRIMER_LEFT_4_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_4_SEQUENCE=GATAGGTGTGAGCTGGTGGAG
PRIMER_LEFT_4=77,22
PRIMER_RIGHT_4=222,21
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=59.736
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_02546 Ps_contig_mira-and-tgicl-ass_franssen_22228
SEQUENCE=GTCGGGGAGAACCCAAATACACTTGTAAGAAAAACAATGAGGTCCCTAATGGCCTCTGCTGCTCTCATTCTTGCATTAGCCATGCTCTTTCTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCACCACCAAAGGATCCATACCATTACNCATCTCCTCCACCTCCACCAGCTCACACCTATCCCTCAA
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2868
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.261226
PRIMER_LEFT_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_SEQUENCE=GAGGTGGAGGAGATGNGTAATG
PRIMER_LEFT=77,22
PRIMER_RIGHT=204,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=60.261
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.75
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=1.0151
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.989557
PRIMER_LEFT_1_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_1_SEQUENCE=GGAGGTGGAGGAGATGNGTAAT
PRIMER_LEFT_1=77,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=60.990
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=1.2872
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=1.261614
PRIMER_LEFT_2_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_2_SEQUENCE=TTGAGGGATAGGTGTGAGCTG
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=228,21
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=60.262
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=1.2894
PRIMER_LEFT_3_PENALTY=0.025566
PRIMER_RIGHT_3_PENALTY=1.263803
PRIMER_LEFT_3_SEQUENCE=AGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_3_SEQUENCE=GATAGGTGTGAGCTGGTGGAG
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=222,21
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=59.736
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=57.143
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=1.2959
PRIMER_LEFT_4_PENALTY=1.034654
PRIMER_RIGHT_4_PENALTY=0.261226
PRIMER_LEFT_4_SEQUENCE=TAGCCATGCTCTTTCTCAGTTTC
PRIMER_RIGHT_4_SEQUENCE=GAGGTGGAGGAGATGNGTAATG
PRIMER_LEFT_4=76,23
PRIMER_RIGHT_4=204,22
PRIMER_LEFT_4_TM=60.035
PRIMER_RIGHT_4_TM=60.261
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.75
PRIMER_PAIR_4_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_4=129
=
PRIMER_SEQUENCE_ID=SSR_02547 Ps_contig_mira-and-tgicl-ass_franssen_22231
SEQUENCE=TTATTGTATAAACCAACGGAAAGACTTCTTCTTTTGTATCTTTTTCCCTAAAATAACCTTTCTTCTCTCCCTCCATGAATAGTTGTTGTTGTAGCTGTTGCCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTTGTTACTTCCTCTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTAATTTTGTCAAGGAAACCCTTCTTTTCCTCAGTACCGGTTGTTCCTTCAACAACCTCAACTTTCTCAACTGGCACCGAAGTGTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTTCTCACCATCTTCTCCTTCCTCCTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGRTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCACCTTGTGAA
TARGET=288,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02548 Ps_contig_mira-and-tgicl-ass_franssen_22233
SEQUENCE=ATAATACTTGTAAACCAACCAACCCACTCATCTTCTAAAACCCTAAACCAAGTGTTACAGTTGGTGGAGGTACTGATCTTGTAAGGAATAGCTAACACCGCATTTGCCAGGGAGAGCAGCAGCATTGTYAGTATTCAATCTAGAAATAGAACCGGCAGCTGATTTCAAGCAGTTACAGGCAGCCTGACGATCCGGCGTGGTGGTGGCGGCAGCAAGAAGCTTCTTCACTCCTGCACAGCATGGCGGTGGAGGACTGGCTATTAGTTAGGGAGTCTTAGGAAAGAATTAAAGGTAAGGCAGTGGANCCAATCACGGATAC
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02549 Ps_contig_mira-and-tgicl-ass_franssen_22237
SEQUENCE=CAGAGACACACAAACAAGACACAGAAAACCAGATCCAGTAAAGAACAACAGATCCATTACAGAACGAGTACCCAATTCTAGATCTAGATTGATCAATCAGTAGATCTCAACATCTAGAATTAAACTTAAATAATCATAAACGATAACCATCTAAAACACTACTCCCTCCAGTTTCCACCGCCGCCGCCGCCTCCACGAGAATAACCACCTCCTCCACCACTTCCTCCATAACCACGATCCCCATCCCGCGTACTCTGCGTTGA
TARGET=178,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2491
PRIMER_LEFT_PENALTY=0.506150
PRIMER_RIGHT_PENALTY=0.742980
PRIMER_LEFT_SEQUENCE=TCCATTACAGAACGAGTACCCA
PRIMER_RIGHT_SEQUENCE=GATCGTGGTTATGGAGGAAGTG
PRIMER_LEFT=52,22
PRIMER_RIGHT=238,22
PRIMER_LEFT_TM=59.494
PRIMER_RIGHT_TM=60.743
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=1.5084
PRIMER_LEFT_1_PENALTY=0.506150
PRIMER_RIGHT_1_PENALTY=1.002229
PRIMER_LEFT_1_SEQUENCE=TCCATTACAGAACGAGTACCCA
PRIMER_RIGHT_1_SEQUENCE=AAGTGGTGGAGGAGGTGGTTAT
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=221,22
PRIMER_LEFT_1_TM=59.494
PRIMER_RIGHT_1_TM=61.002
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=1.5913
PRIMER_LEFT_2_PENALTY=0.506150
PRIMER_RIGHT_2_PENALTY=1.085187
PRIMER_LEFT_2_SEQUENCE=TCCATTACAGAACGAGTACCCA
PRIMER_RIGHT_2_SEQUENCE=GGTGGAGGAGGTGGTTATTCTC
PRIMER_LEFT_2=52,22
PRIMER_RIGHT_2=217,22
PRIMER_LEFT_2_TM=59.494
PRIMER_RIGHT_2_TM=61.085
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=1.7256
PRIMER_LEFT_3_PENALTY=0.982604
PRIMER_RIGHT_3_PENALTY=0.742980
PRIMER_LEFT_3_SEQUENCE=CCATTACAGAACGAGTACCCAA
PRIMER_RIGHT_3_SEQUENCE=GATCGTGGTTATGGAGGAAGTG
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=59.017
PRIMER_RIGHT_3_TM=60.743
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=1.7753
PRIMER_LEFT_4_PENALTY=0.506150
PRIMER_RIGHT_4_PENALTY=1.269132
PRIMER_LEFT_4_SEQUENCE=TCCATTACAGAACGAGTACCCA
PRIMER_RIGHT_4_SEQUENCE=AGTGGTGGAGGAGGTGGTTAT
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=220,21
PRIMER_LEFT_4_TM=59.494
PRIMER_RIGHT_4_TM=59.731
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=169
=
PRIMER_SEQUENCE_ID=SSR_02550 Ps_contig_mira-and-tgicl-ass_franssen_22240
SEQUENCE=CTTTAACTAAAACAATACACGCCAAATTTTAATTTAAACTATTCTTTATTCATCATATAATGCCTTAATGATTAATTTTTGTATAAGATGACCATCAATATCTCTAGTGCATTGGCTAAGATAGTCCATCCATCCATTTGGAGGATGAATATCYTCACTGATTTTCTCATATTCAACAGTCCATTTAGCAACAGCAGTGCCAYCAGCCTTATCAACTACTTCTTCAATACTTTCATTACATG
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02551 Ps_contig_mira-and-tgicl-ass_franssen_22241
SEQUENCE=AGATTGTGAACAAGGACATAGTGATTTTGTTTGGTCATGTAGTTGGAATTMATCATGTTCCAGACACAGGAAGATTTCCCWATAATGCCACTATTGAGCACCTCATTTGAGCTGAGGCCAACCAATTTCTTTGAGAGGAATCCAGTTCTTAAAACACTTTCACCAAGAGATGTTGCATGGCCTGGTTGCTCCAATTAAAACTATCCAACATTCTGTATGGAACTGRTTTGCATTGGGCCTTGTGGTCATATGAATAAATGTTGTTTGTTTGTTTGTTGCATGTGGAAGAATAATGTATGGTTTCAACTTATTTTAATTTGAATGAATGAATGAGAGGTTAATTTTAAAAACT
TARGET=261,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02552 Ps_contig_mira-and-tgicl-ass_franssen_22241
SEQUENCE=AGATTGTGAACAAGGACATAGTGATTTTGTTTGGTCATGTAGTTGGAATTMATCATGTTCCAGACACAGGAAGATTTCCCWATAATGCCACTATTGAGCACCTCATTTGAGCTGAGGCCAACCAATTTCTTTGAGAGGAATCCAGTTCTTAAAACACTTTCACCAAGAGATGTTGCATGGCCTGGTTGCTCCAATTAAAACTATCCAACATTCTGTATGGAACTGRTTTGCATTGGGCCTTGTGGTCATATGAATAAATGTTGTTTGTTTGTTTGTTGCATGTGGAAGAATAATGTATGGTTTCAACTTATTTTAATTTGAATGAATGAATGAGAGGTTAATTTTAAAAACT
TARGET=319,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02553 Ps_contig_mira-and-tgicl-ass_franssen_22255
SEQUENCE=CACACTCTGCACCAACAACCCAACCAAATTGACGATAACGAGAACACCCACCAAGAACCCACCAATAACAATCCTAGAAAACACACCATAACCATCACCACCATGCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCAACCTCCTCCACCTTACCACCACTAGATAGTCAATCATTGGCA
TARGET=157,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1494
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=0.136017
PRIMER_LEFT_SEQUENCE=ACCCAACCAAATTGACGATAAC
PRIMER_RIGHT_SEQUENCE=GGTGGAGGAGGTTGATTTGTAG
PRIMER_LEFT=17,22
PRIMER_RIGHT=220,22
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=59.864
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=0.1494
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=ACCCAACCAAATTGACGATAAC
PRIMER_RIGHT_1_SEQUENCE=AGGTGGAGGAGGTTGATTTGTA
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=221,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.1558
PRIMER_LEFT_2_PENALTY=0.019805
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CCAAGAACCCACCAATAACAAT
PRIMER_RIGHT_2_SEQUENCE=GGTGGAGGAGGTTGATTTGTAG
PRIMER_LEFT_2=50,22
PRIMER_RIGHT_2=220,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.1558
PRIMER_LEFT_3_PENALTY=0.019805
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CCAAGAACCCACCAATAACAAT
PRIMER_RIGHT_3_SEQUENCE=AGGTGGAGGAGGTTGATTTGTA
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=221,22
PRIMER_LEFT_3_TM=59.980
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.3579
PRIMER_LEFT_4_PENALTY=0.013401
PRIMER_RIGHT_4_PENALTY=0.344527
PRIMER_LEFT_4_SEQUENCE=ACCCAACCAAATTGACGATAAC
PRIMER_RIGHT_4_SEQUENCE=TAAGGTGGAGGAGGTTGATTTG
PRIMER_LEFT_4=17,22
PRIMER_RIGHT_4=223,22
PRIMER_LEFT_4_TM=59.987
PRIMER_RIGHT_4_TM=60.345
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_02554 Ps_contig_mira-and-tgicl-ass_franssen_22263
SEQUENCE=ACAWAWTTATTTATTTATTAATTTATTGAAGGAGWGGTATACATGACTTACAATCTCACACTTTTAGTTCCACAACAAAAGAAAAGGAAAAAAACAAAACAAGAAAATTACATTGGCAGCCAAATTAAGCACACAGGATGATTATATAGTATACATAACAACACTACTTGTTGATGATTATATTAGTTTTAGTTGTAATCAGGATGACGMCCAAAACACGTAACCCCTNAAGGCGTCCTTAGAAAAACGCCCTA
TARGET=7,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02555 Ps_contig_mira-and-tgicl-ass_franssen_22283
SEQUENCE=ACGGGGGAAACAAGAAAGCAGATAACAGAAAAAAAGGTGTGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGCAGCAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTGCTCTGCTTGCCTTATCTCCCTCCTCCTCGCCTCCTCCACCACCACCTTCCTTCCTTCCTTTCCCCTTTACCCTAAACTATTTTCAGAGTCGCGGTAATTTTCTCTTC
TARGET=41,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4779
PRIMER_LEFT_PENALTY=0.390906
PRIMER_RIGHT_PENALTY=0.087031
PRIMER_LEFT_SEQUENCE=GGGGAAACAAGAAAGCAGATAA
PRIMER_RIGHT_SEQUENCE=AGAGAAAATTACCGCGACTCTG
PRIMER_LEFT=3,22
PRIMER_RIGHT=241,22
PRIMER_LEFT_TM=59.609
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.5478
PRIMER_LEFT_1_PENALTY=0.390906
PRIMER_RIGHT_1_PENALTY=0.156918
PRIMER_LEFT_1_SEQUENCE=GGGGAAACAAGAAAGCAGATAA
PRIMER_RIGHT_1_SEQUENCE=TTAGGGTAAAGGGGAAAGGAAG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=59.609
PRIMER_RIGHT_1_TM=59.843
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.5549
PRIMER_LEFT_2_PENALTY=0.390906
PRIMER_RIGHT_2_PENALTY=0.164039
PRIMER_LEFT_2_SEQUENCE=GGGGAAACAAGAAAGCAGATAA
PRIMER_RIGHT_2_SEQUENCE=TTTAGGGTAAAGGGGAAAGGAA
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=59.609
PRIMER_RIGHT_2_TM=60.164
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.5840
PRIMER_LEFT_3_PENALTY=0.390906
PRIMER_RIGHT_3_PENALTY=0.193133
PRIMER_LEFT_3_SEQUENCE=GGGGAAACAAGAAAGCAGATAA
PRIMER_RIGHT_3_SEQUENCE=AGAGGAGGGACTAACACTGTGC
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=106,22
PRIMER_LEFT_3_TM=59.609
PRIMER_RIGHT_3_TM=59.807
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=104
PRIMER_PAIR_PENALTY_4=0.6474
PRIMER_LEFT_4_PENALTY=0.390906
PRIMER_RIGHT_4_PENALTY=0.256503
PRIMER_LEFT_4_SEQUENCE=GGGGAAACAAGAAAGCAGATAA
PRIMER_RIGHT_4_SEQUENCE=AGAAAATTACCGCGACTCTGAA
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=239,22
PRIMER_LEFT_4_TM=59.609
PRIMER_RIGHT_4_TM=60.257
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_02556 Ps_contig_mira-and-tgicl-ass_franssen_22283
SEQUENCE=ACGGGGGAAACAAGAAAGCAGATAACAGAAAAAAAGGTGTGAGAGAGAGAGAAACAAGAGTTAATGGCAATGGCAGCAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTGCTCTGCTTGCCTTATCTCCCTCCTCCTCGCCTCCTCCACCACCACCTTCCTTCCTTCCTTTCCCCTTTACCCTAAACTATTTTCAGAGTCGCGGTAATTTTCTCTTC
TARGET=182,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2802
PRIMER_LEFT_PENALTY=0.193133
PRIMER_RIGHT_PENALTY=0.087031
PRIMER_LEFT_SEQUENCE=GCACAGTGTTAGTCCCTCCTCT
PRIMER_RIGHT_SEQUENCE=AGAGAAAATTACCGCGACTCTG
PRIMER_LEFT=85,22
PRIMER_RIGHT=241,22
PRIMER_LEFT_TM=59.807
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.2802
PRIMER_LEFT_1_PENALTY=0.193133
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=AGCACAGTGTTAGTCCCTCCTC
PRIMER_RIGHT_1_SEQUENCE=AGAGAAAATTACCGCGACTCTG
PRIMER_LEFT_1=84,22
PRIMER_RIGHT_1=241,22
PRIMER_LEFT_1_TM=59.807
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=0.4496
PRIMER_LEFT_2_PENALTY=0.193133
PRIMER_RIGHT_2_PENALTY=0.256503
PRIMER_LEFT_2_SEQUENCE=GCACAGTGTTAGTCCCTCCTCT
PRIMER_RIGHT_2_SEQUENCE=AGAAAATTACCGCGACTCTGAA
PRIMER_LEFT_2=85,22
PRIMER_RIGHT_2=239,22
PRIMER_LEFT_2_TM=59.807
PRIMER_RIGHT_2_TM=60.257
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.4496
PRIMER_LEFT_3_PENALTY=0.193133
PRIMER_RIGHT_3_PENALTY=0.256503
PRIMER_LEFT_3_SEQUENCE=AGCACAGTGTTAGTCCCTCCTC
PRIMER_RIGHT_3_SEQUENCE=AGAAAATTACCGCGACTCTGAA
PRIMER_LEFT_3=84,22
PRIMER_RIGHT_3=239,22
PRIMER_LEFT_3_TM=59.807
PRIMER_RIGHT_3_TM=60.257
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.4779
PRIMER_LEFT_4_PENALTY=0.390906
PRIMER_RIGHT_4_PENALTY=0.087031
PRIMER_LEFT_4_SEQUENCE=GGGGAAACAAGAAAGCAGATAA
PRIMER_RIGHT_4_SEQUENCE=AGAGAAAATTACCGCGACTCTG
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=241,22
PRIMER_LEFT_4_TM=59.609
PRIMER_RIGHT_4_TM=59.913
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_02557 Ps_contig_mira-and-tgicl-ass_franssen_22296
SEQUENCE=TATGGTTTTTTAACCGGAGGTGGTGGTGATTTATATTTATACACCGGTGGAGGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGACTTATATTTGTAAATCGGTGGTGGAGGAGAGANTTANAGATTCTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAGGTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTGCTTGGAGGAGTGGACGAAGGGTGAGAGAGAGTTATTGTACAATGACTAAGTTAGGTAGG
TARGET=111,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1522
PRIMER_LEFT_PENALTY=2.146620
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=CGGAGGTGGTGGTGATTTATATT
PRIMER_RIGHT_SEQUENCE=CTCACCTCCTCCACCTTATCAC
PRIMER_LEFT=14,23
PRIMER_RIGHT=155,22
PRIMER_LEFT_TM=61.147
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.8000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=142
PRIMER_PAIR_PENALTY_1=2.1522
PRIMER_LEFT_1_PENALTY=2.146620
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=CGGAGGTGGTGGTGATTTATATT
PRIMER_RIGHT_1_SEQUENCE=CTCACCTCCTCCACCTTATCAC
PRIMER_LEFT_1=14,23
PRIMER_RIGHT_1=265,22
PRIMER_LEFT_1_TM=61.147
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=2.1522
PRIMER_LEFT_2_PENALTY=2.146620
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=CGGAGGTGGTGGTGATTTATATT
PRIMER_RIGHT_2_SEQUENCE=TCACCTCCTCCACCTTATCACT
PRIMER_LEFT_2=14,23
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=61.147
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=141
PRIMER_PAIR_PENALTY_3=2.1522
PRIMER_LEFT_3_PENALTY=2.146620
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=CGGAGGTGGTGGTGATTTATATT
PRIMER_RIGHT_3_SEQUENCE=TCACCTCCTCCACCTTATCACT
PRIMER_LEFT_3=14,23
PRIMER_RIGHT_3=264,22
PRIMER_LEFT_3_TM=61.147
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=2.1522
PRIMER_LEFT_4_PENALTY=2.146620
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=CGGAGGTGGTGGTGATTTATATT
PRIMER_RIGHT_4_SEQUENCE=ACTCACCTCCTCCACCTTATCA
PRIMER_LEFT_4=14,23
PRIMER_RIGHT_4=156,22
PRIMER_LEFT_4_TM=61.147
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_02558 Ps_contig_mira-and-tgicl-ass_franssen_22296
SEQUENCE=TATGGTTTTTTAACCGGAGGTGGTGGTGATTTATATTTATACACCGGTGGAGGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGACTTATATTTGTAAATCGGTGGTGGAGGAGAGANTTANAGATTCTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAGGTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTGCTTGGAGGAGTGGACGAAGGGTGAGAGAGAGTTATTGTACAATGACTAAGTTAGGTAGG
TARGET=162,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0724
PRIMER_LEFT_PENALTY=0.066849
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_SEQUENCE=CACTCACCTCCTCCACCTTATC
PRIMER_LEFT=96,22
PRIMER_RIGHT=267,22
PRIMER_LEFT_TM=60.067
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.0724
PRIMER_LEFT_1_PENALTY=0.066849
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_1_SEQUENCE=CACTCACCTCCTCCACCTTATC
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=315,22
PRIMER_LEFT_1_TM=60.067
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=220
PRIMER_PAIR_PENALTY_2=0.0724
PRIMER_LEFT_2_PENALTY=0.066849
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_2_SEQUENCE=CTCACCTCCTCCACCTTATCAC
PRIMER_LEFT_2=96,22
PRIMER_RIGHT_2=265,22
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.0724
PRIMER_LEFT_3_PENALTY=0.066849
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_3_SEQUENCE=TCACCTCCTCCACCTTATCACT
PRIMER_LEFT_3=96,22
PRIMER_RIGHT_3=264,22
PRIMER_LEFT_3_TM=60.067
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=0.0724
PRIMER_LEFT_4_PENALTY=0.066849
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_4_SEQUENCE=ACTCACCTCCTCCACCTTATCA
PRIMER_LEFT_4=96,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=60.067
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_02559 Ps_contig_mira-and-tgicl-ass_franssen_22296
SEQUENCE=TATGGTTTTTTAACCGGAGGTGGTGGTGATTTATATTTATACACCGGTGGAGGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGACTTATATTTGTAAATCGGTGGTGGAGGAGAGANTTANAGATTCTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAGGTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTGCTTGGAGGAGTGGACGAAGGGTGAGAGAGAGTTATTGTACAATGACTAAGTTAGGTAGG
TARGET=221,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0724
PRIMER_LEFT_PENALTY=0.066849
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_SEQUENCE=CACTCACCTCCTCCACCTTATC
PRIMER_LEFT=96,22
PRIMER_RIGHT=267,22
PRIMER_LEFT_TM=60.067
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.0724
PRIMER_LEFT_1_PENALTY=0.066849
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_1_SEQUENCE=CACTCACCTCCTCCACCTTATC
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=315,22
PRIMER_LEFT_1_TM=60.067
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=220
PRIMER_PAIR_PENALTY_2=0.0724
PRIMER_LEFT_2_PENALTY=0.066849
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_2_SEQUENCE=CTCACCTCCTCCACCTTATCAC
PRIMER_LEFT_2=96,22
PRIMER_RIGHT_2=265,22
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.0724
PRIMER_LEFT_3_PENALTY=0.066849
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_3_SEQUENCE=TCACCTCCTCCACCTTATCACT
PRIMER_LEFT_3=96,22
PRIMER_RIGHT_3=264,22
PRIMER_LEFT_3_TM=60.067
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=0.0724
PRIMER_LEFT_4_PENALTY=0.066849
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_4_SEQUENCE=ACTCACCTCCTCCACCTTATCA
PRIMER_LEFT_4=96,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=60.067
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_02560 Ps_contig_mira-and-tgicl-ass_franssen_22296
SEQUENCE=TATGGTTTTTTAACCGGAGGTGGTGGTGATTTATATTTATACACCGGTGGAGGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGACTTATATTTGTAAATCGGTGGTGGAGGAGAGANTTANAGATTCTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAGGTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTGCTTGGAGGAGTGGACGAAGGGTGAGAGAGAGTTATTGTACAATGACTAAGTTAGGTAGG
TARGET=272,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0724
PRIMER_LEFT_PENALTY=0.066849
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_SEQUENCE=CACTCACCTCCTCCACCTTATC
PRIMER_LEFT=96,22
PRIMER_RIGHT=315,22
PRIMER_LEFT_TM=60.067
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.0724
PRIMER_LEFT_1_PENALTY=0.066849
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_1_SEQUENCE=ACTCACCTCCTCCACCTTATCA
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=314,22
PRIMER_LEFT_1_TM=60.067
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=0.2402
PRIMER_LEFT_2_PENALTY=0.066849
PRIMER_RIGHT_2_PENALTY=0.173354
PRIMER_LEFT_2_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_2_SEQUENCE=TCACCTCCTCCACCTTATCATT
PRIMER_LEFT_2=96,22
PRIMER_RIGHT_2=312,22
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=59.827
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=217
PRIMER_PAIR_PENALTY_3=0.3283
PRIMER_LEFT_3_PENALTY=0.322707
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=GTACGACTTCTTTGGTGGTGGT
PRIMER_RIGHT_3_SEQUENCE=CACTCACCTCCTCCACCTTATC
PRIMER_LEFT_3=98,22
PRIMER_RIGHT_3=315,22
PRIMER_LEFT_3_TM=60.323
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.3283
PRIMER_LEFT_4_PENALTY=0.322707
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=GTACGACTTCTTTGGTGGTGGT
PRIMER_RIGHT_4_SEQUENCE=ACTCACCTCCTCCACCTTATCA
PRIMER_LEFT_4=98,22
PRIMER_RIGHT_4=314,22
PRIMER_LEFT_4_TM=60.323
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_02561 Ps_contig_mira-and-tgicl-ass_franssen_22296
SEQUENCE=TATGGTTTTTTAACCGGAGGTGGTGGTGATTTATATTTATACACCGGTGGAGGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGACTTATATTTGTAAATCGGTGGTGGAGGAGAGANTTANAGATTCTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGAGGTGAGTGCACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAGGTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTGCTTGGAGGAGTGGACGAAGGGTGAGAGAGAGTTATTGTACAATGACTAAGTTAGGTAGG
TARGET=351,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3527
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.347129
PRIMER_LEFT_SEQUENCE=GTGATAAGGTGGAGGAGGTGAG
PRIMER_RIGHT_SEQUENCE=CCTCCAAGCAACAATTACCTCT
PRIMER_LEFT=244,22
PRIMER_RIGHT=394,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=59.653
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=0.3527
PRIMER_LEFT_1_PENALTY=0.005574
PRIMER_RIGHT_1_PENALTY=0.347129
PRIMER_LEFT_1_SEQUENCE=GTGATAAGGTGGAGGAGGTGAG
PRIMER_RIGHT_1_SEQUENCE=CCTCCAAGCAACAATTACCTCT
PRIMER_LEFT_1=134,22
PRIMER_RIGHT_1=394,22
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=59.653
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.3527
PRIMER_LEFT_2_PENALTY=0.005574
PRIMER_RIGHT_2_PENALTY=0.347129
PRIMER_LEFT_2_SEQUENCE=GTGATAAGGTGGAGGAGGTGAG
PRIMER_RIGHT_2_SEQUENCE=CTCCTCCAAGCAACAATTACCT
PRIMER_LEFT_2=244,22
PRIMER_RIGHT_2=396,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=59.653
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=153
PRIMER_PAIR_PENALTY_3=0.3527
PRIMER_LEFT_3_PENALTY=0.005574
PRIMER_RIGHT_3_PENALTY=0.347129
PRIMER_LEFT_3_SEQUENCE=GTGATAAGGTGGAGGAGGTGAG
PRIMER_RIGHT_3_SEQUENCE=CTCCTCCAAGCAACAATTACCT
PRIMER_LEFT_3=134,22
PRIMER_RIGHT_3=396,22
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=59.653
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=0.4140
PRIMER_LEFT_4_PENALTY=0.066849
PRIMER_RIGHT_4_PENALTY=0.347129
PRIMER_LEFT_4_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_RIGHT_4_SEQUENCE=CCTCCAAGCAACAATTACCTCT
PRIMER_LEFT_4=96,22
PRIMER_RIGHT_4=394,22
PRIMER_LEFT_4_TM=60.067
PRIMER_RIGHT_4_TM=59.653
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_02562 Ps_contig_mira-and-tgicl-ass_franssen_22300
SEQUENCE=CCCCCGTTTTTTAAACGGTTTACCCTACGGTTACCTACAACCCCCAACTTTACCCACTTTGGTTTTAAGGTACGTTCCTAGGAGTTCTCAACTGGAACTTCCTTCCTGCTGCTGGAGCTTCCACTGATTCAACTGTTTTGTTTCTCAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTTACTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTTCACTTCTTCAACCGATTCCTCAGTTTACTTTAGGTGCTTCWAAGTTCTCTTCCTTTGCTTCCTCTGTTACCACTTCCTTGTTCTCAACTTCAAGTGGATCTTGGGTTTCGGGANCATCTACAGCTTCTCCTGCTGCAGTTGTTTCTGTTTGGTTTTTCGGTTGTTTCTTCTGTTGGTACTTCGGCGGNAGGGTAACTTCGGTA
TARGET=168,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02563 Ps_contig_mira-and-tgicl-ass_franssen_22316
SEQUENCE=ATGCTGATGTGGAGCCAAGTGCCATATAAATAACCATATCCAAAAGCCATACCATAATAACAATAATAAACTTTAATATAGGAAAGGAAACTAAAGAMRTRWYTMATRGAAAAGCTTTTCAACCTTGATTGACATGCAAAATGGTAGACCCGATCCCAACATCTAGAACCATTAATTTAAACTTCCGTTTTTTACCAGTTCCAGTCACACCCATTCCATTGGTTTAAGCTTCAGGACTCTCAACTGGAACTTCCTCTGCTGCTGGAGCTTCCACTGATTCAACTGTTTTGTTCTCAATCACATCCTCTGCAGTTGCCGGTGGTGGTGGTGCTGACTCTATTTTCAGTAGTTGCTA
TARGET=318,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02564 Ps_contig_mira-and-tgicl-ass_franssen_22319
SEQUENCE=TCTCCAAATGGATGGATGGACTATCTTAACCAATGCACTAGAGATATTGATGGTCATCTTATCAAAAGAGAAAACGTTGCTATATGAGTCATTCAGGCAYTATAGTAATTAAATAAAGAGAGTTTGTTTAATTATTATTAGCGTGATGTTTGTAATGACATGCATGTAACCTATATAAGCATGCATGTTTACTTCTCTTGATGCTTCCTTTGTATGGAATAAATGTGTCGGTCATGTCATGATGAATGGGTCTTATTATATTATTGTTGTAATGACAAATTAAATTNTTTCTGAAATT
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02565 Ps_contig_mira-and-tgicl-ass_franssen_22330
SEQUENCE=ATTTTCCTTCAGCAAACCCATTGTTGCTGTTCTTAACTGCTCTCTCTGCTGCTTTCTTTGCCATCGTTTTCTGGGACTCGTCCACGTGGATCTTGATCTGACTTTTCATTGACGGACGGACGGGATCTGTTTGATTTAAATTCTGAGTAGCGTTTTATTTTGTTTTCATGTGTCTATTTATACTTTTTCGTTCATGTGTTTGTTGATTCGACGGACGGGATCGATCTTCATGATTATGATGATGTATTTTATTTTGTGTATTCTATTTTGTCTTTGGTATTACCACTACGGTTGTTATGGTAAG
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2397
PRIMER_LEFT_PENALTY=0.102789
PRIMER_RIGHT_PENALTY=0.136881
PRIMER_LEFT_SEQUENCE=ACTGCTCTCTCTGCTGCTTTCT
PRIMER_RIGHT_SEQUENCE=ATAATCATGAAGATCGATCCCG
PRIMER_LEFT=35,22
PRIMER_RIGHT=236,22
PRIMER_LEFT_TM=60.103
PRIMER_RIGHT_TM=60.137
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.6014
PRIMER_LEFT_1_PENALTY=0.464556
PRIMER_RIGHT_1_PENALTY=0.136881
PRIMER_LEFT_1_SEQUENCE=AACTGCTCTCTCTGCTGCTTTC
PRIMER_RIGHT_1_SEQUENCE=ATAATCATGAAGATCGATCCCG
PRIMER_LEFT_1=34,22
PRIMER_RIGHT_1=236,22
PRIMER_LEFT_1_TM=60.465
PRIMER_RIGHT_1_TM=60.137
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.7253
PRIMER_LEFT_2_PENALTY=0.588441
PRIMER_RIGHT_2_PENALTY=0.136881
PRIMER_LEFT_2_SEQUENCE=CTGCTCTCTCTGCTGCTTTCTT
PRIMER_RIGHT_2_SEQUENCE=ATAATCATGAAGATCGATCCCG
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=236,22
PRIMER_LEFT_2_TM=60.588
PRIMER_RIGHT_2_TM=60.137
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.9638
PRIMER_LEFT_3_PENALTY=0.826889
PRIMER_RIGHT_3_PENALTY=0.136881
PRIMER_LEFT_3_SEQUENCE=TCTTAACTGCTCTCTCTGCTGC
PRIMER_RIGHT_3_SEQUENCE=ATAATCATGAAGATCGATCCCG
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=236,22
PRIMER_LEFT_3_TM=59.173
PRIMER_RIGHT_3_TM=60.137
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=1.0785
PRIMER_LEFT_4_PENALTY=0.941586
PRIMER_RIGHT_4_PENALTY=0.136881
PRIMER_LEFT_4_SEQUENCE=GCTCTCTCTGCTGCTTTCTTTG
PRIMER_RIGHT_4_SEQUENCE=ATAATCATGAAGATCGATCCCG
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=236,22
PRIMER_LEFT_4_TM=60.942
PRIMER_RIGHT_4_TM=60.137
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_02566 Ps_contig_mira-and-tgicl-ass_franssen_22332
SEQUENCE=AAAAGTTTTTTTTTTACTTTATAATGAAATATCCNTTTATTGATTTGTCATCACATGNTTTAAGTCACACTCTTGAACCGATCACACAGAGAAACGAAAACAGAAAGCAAATATTACAAGCATCACACTAATTAATTATAAGATGATCAGATAAGCCAACTCCATCCATAAACCATGCCATCTTATTTGTTACTTTCTTTTTAATCTTTTCGAGCGGCCCTTGCCTTGGAAAGATGCGCATCAACATCTCTAGTGCATTGGCTAACGTAGTCCATCCATCCATTTGKAGGAGTGAATATCCTCGTTGATTTTCTTCAAATTCAACGGTCCATTTAGCAACAGCAGTGCCATCTTACAAAGCCTTATCAATTACTTGAAGGGTGGAGACTTTAAA
TARGET=271,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02567 Ps_contig_mira-and-tgicl-ass_franssen_22336
SEQUENCE=TTTATTACTGAGAATGTTGATATTATCTTTAGTTACCGTTGTTGGAAGCACCACCATGTCCACCGTGTCCACCTCCACCATGGTAACCACCACCACCATTATGGTAACCTCCACCGCCATTGTAWCCWCCTCCACCATTATGGTAACCACCACCACGTCCATAACCTCCATATTTAGCATCGTTTACTTCATCTGACTTTTCAACAACCTCCTCAGTTAATTCCCTAGCTGACACCTCTGATGAAATAAGAAGAACCATGGACCAATAGGCCAAGAATGAGCTATTTCGTCTTTTCGAGGAACTTCCTA
TARGET=86,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02568 Ps_contig_mira-and-tgicl-ass_franssen_22342
SEQUENCE=TGTGTGTGAGTTTATTTCTTCTTCAACAAAAGACCACTATTACTTTGTTTGGGTTGGTTGGTTTTATTTTTCTTTCAATCGGGAAAGTAAAGAGAGTTAGGGTTTCATGTAGAACATGGTYTCTTCTCTCATGTGAATCAGCTCAATTGTTTTGTTGTWGGTGCGCAGTGTGTGTGTGTTGTAAATTGCGAACTACAGAATTTGATTCCTACTAAATAAAAAAACTCAAATCTATTTTGWAACG
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02569 Ps_contig_mira-and-tgicl-ass_franssen_22342
SEQUENCE=TGTGTGTGAGTTTATTTCTTCTTCAACAAAAGACCACTATTACTTTGTTTGGGTTGGTTGGTTTTATTTTTCTTTCAATCGGGAAAGTAAAGAGAGTTAGGGTTTCATGTAGAACATGGTYTCTTCTCTCATGTGAATCAGCTCAATTGTTTTGTTGTWGGTGCGCAGTGTGTGTGTGTTGTAAATTGCGAACTACAGAATTTGATTCCTACTAAATAAAAAAACTCAAATCTATTTTGWAACG
TARGET=168,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02570 Ps_contig_mira-and-tgicl-ass_franssen_22359
SEQUENCE=GTTCGGGTTAWTCTAACACTAAACCTAAGCATTTCAACTATTTATATATATATTCACAGAGATCATATCGTATATCATAAGATGACTGGTTTAAATTACAGCAACATCTTTAGTGGGTACGATGGTGGAAACAATGGAACTGTTGATGCATTTAACAACAAGTGTGGCTATCAAGATTATGGTGAGGCTGAACATCAAAACTGGTTGCACAAATTACCGATGGTARG
TARGET=43,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02571 Ps_contig_mira-and-tgicl-ass_franssen_22362
SEQUENCE=GTCGGGGGGKCCCCCACAAAACCCTTTCAAATTCACCTCTCTCTCTCTCTCTCTTCCGTATTTTCGTTTTCTAGGTCATTTCAATTTCACGAAATGAGAGAAATTCTTCATATCCAAGGTGGCCAATGCGGAAACCAGATCGGAGCCAAATTCGTGGGAAGTTGTTTGCGCCGAACATGGAATCCGACCCTACGGAAGGTATGGGT
TARGET=37,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02572 Ps_contig_mira-and-tgicl-ass_franssen_22408
SEQUENCE=CCACCTCCATAGTGTCCACCACCATAGTTTTGTTTCCTTACCGTTCCTATATTTGGCATCATTTACTTCATTTGACTTTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACYSYMACCTCCACCAACACCGATTTACAAATATAAATCTCCTCCACCTGTTTACTCGCCACCACCCGTTTACAAATATAAATCTCCTCCTCCACCTGTTTACTCACCACCTCCACCACACTATGTCTACTCTTCGCCTCCTCCACCTTACCACCACTAGATAGCCCATCATTGGCATCACACTATATGGATCTAAGCAG
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02573 Ps_contig_mira-and-tgicl-ass_franssen_22408
SEQUENCE=CCACCTCCATAGTGTCCACCACCATAGTTTTGTTTCCTTACCGTTCCTATATTTGGCATCATTTACTTCATTTGACTTTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACYSYMACCTCCACCAACACCGATTTACAAATATAAATCTCCTCCACCTGTTTACTCGCCACCACCCGTTTACAAATATAAATCTCCTCCTCCACCTGTTTACTCACCACCTCCACCACACTATGTCTACTCTTCGCCTCCTCCACCTTACCACCACTAGATAGCCCATCATTGGCATCACACTATATGGATCTAAGCAG
TARGET=166,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02574 Ps_contig_mira-and-tgicl-ass_franssen_22451
SEQUENCE=TTCGGGATCTTTCTTCATATCTCTCTCACTTTCACTCCCAAACCAAAACACACACACATCATTGGGTTTGCAGGAGGTATTTTGAGGAGCATGCTGCGAAAGTTAAGACTCTAACAGAGAGTCCATACAAATAGAAAACTTGTCTTATTCCTTTTAGTTAGGGTATTTTAGTAAACGAAACGGCCGAAACGGTACCCCCTAAACCGTTTCCCGGTTTTACCGGGGTAAACCCCCGTTTTAACCGGGGTTTTAAAACCCCCCAACCCCCGGTCCCGTTTCCCCGGGTTTCCCCGGTTTAGGGGGGTTTAAACTTTTTTTTCCGGAACCGGACCCCGTAAAAAGGGGAAAAAAACCGGAAAACCGAACGGGGAAACCGGTTAAAAACCGGG
TARGET=48,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6123
PRIMER_LEFT_PENALTY=0.145769
PRIMER_RIGHT_PENALTY=1.466576
PRIMER_LEFT_SEQUENCE=CTCTCACTTTCACTCCCAAACC
PRIMER_RIGHT_SEQUENCE=CGGCCGTTTCGTTTACTAAAAT
PRIMER_LEFT=22,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=60.146
PRIMER_RIGHT_TM=61.467
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=1.8489
PRIMER_LEFT_1_PENALTY=0.938316
PRIMER_RIGHT_1_PENALTY=0.910607
PRIMER_LEFT_1_SEQUENCE=TCTCTCTCACTTTCACTCCCAA
PRIMER_RIGHT_1_SEQUENCE=GTAAAACCGGGAAACGGTTTAG
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=219,22
PRIMER_LEFT_1_TM=59.062
PRIMER_RIGHT_1_TM=60.911
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=1.9169
PRIMER_LEFT_2_PENALTY=1.006255
PRIMER_RIGHT_2_PENALTY=0.910607
PRIMER_LEFT_2_SEQUENCE=CACTTTCACTCCCAAACCAAA
PRIMER_RIGHT_2_SEQUENCE=GTAAAACCGGGAAACGGTTTAG
PRIMER_LEFT_2=26,21
PRIMER_RIGHT_2=219,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.911
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=2.1600
PRIMER_LEFT_3_PENALTY=1.249414
PRIMER_RIGHT_3_PENALTY=0.910607
PRIMER_LEFT_3_SEQUENCE=CACTTTCACTCCCAAACCAAAA
PRIMER_RIGHT_3_SEQUENCE=GTAAAACCGGGAAACGGTTTAG
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=219,22
PRIMER_LEFT_3_TM=61.249
PRIMER_RIGHT_3_TM=60.911
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=2.3114
PRIMER_LEFT_4_PENALTY=1.400837
PRIMER_RIGHT_4_PENALTY=0.910607
PRIMER_LEFT_4_SEQUENCE=TCTCTCTCACTTTCACTCCCAAA
PRIMER_RIGHT_4_SEQUENCE=GTAAAACCGGGAAACGGTTTAG
PRIMER_LEFT_4=19,23
PRIMER_RIGHT_4=219,22
PRIMER_LEFT_4_TM=60.401
PRIMER_RIGHT_4_TM=60.911
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=201
=
PRIMER_SEQUENCE_ID=SSR_02575 Ps_contig_mira-and-tgicl-ass_franssen_22483
SEQUENCE=GAGTCATAGCATCTCTTTCTTTCTTCTATTATTATATTTTAGTTTCTTCTCAAAATAGGTGCTGGCAAGCTTATTACTGAACTTGGAATCAAAACACCMGCTGAYAAGTTYTACAAACTRTTTRCATYAGAAYTTYMCGAAGTGCAAAACATTTGTGAAAGAGTTCATAAAGGCAAGCTGCATGAAGGTGAAGACTGGCATGACACTGATACAGTTAAACACTGGACTTATGTCATAGGTAGGTAGAGGTACACACATGTTATGAGAGTCTTGAAGAAGTTGATGAACAAAATAAAAAACTGACTCTCAAGCTTTTTGGTGGAGACATTGAGAAGCACTATAAGCTCTTTAAAGTCACCCTTGAAGTAGTTGATAAGGCTGACGGCACTG
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02576 Ps_contig_mira-and-tgicl-ass_franssen_22486
SEQUENCE=AATGAGTTAGCACCACCACCACCAGCAACTGCAGAGGATTGTGATTGAGAACAAAACAGTTGAATCAGTGGAAGCTCCAGCAGCAGAGGAAGTTCCAGTTGAGAATCCTAGAAGCTTAAACCAATGGAATGGGTAGTGACTGGAACTAAAGGTAAAAAACGGAAGTTTAAATTAATGGTTCTAGATGTTGGGATCGGGTCTACCATTTTGCATGTCATCAAGGTTGAAAAGTCTTTTCTATTAATTNTTTCTTTAGTTTCCTTTCCTATTATTAAAGTTTNTTATTGTTATTATGGTTAG
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02577 Ps_contig_mira-and-tgicl-ass_franssen_22499
SEQUENCE=AAGGCAGTAGTTATCAACGCTTTTAGTTTAGTTTTTACGTTCGGGGAAATTCAATCTAAACRAAGAAAGCAGATAACAGAAAAAAAAGGTGTGAGAGAGAGAGAGAAAACAAGAGTTAATGGCAATGGCAGCAAGCACAAGCACAGTGTTAGTCCCTCCTCTTCCTCGGCTACTGACCACCACCACTCGCTGCTCTGTCTTTGCCTTATCCTCCCTCCTCCTCGCCTCTCCACCACCNCTTCCTTCTTCCTTCCCCTTTCCAAATNTTTTCAGAGTCGCGGTATTTCT
TARGET=93,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02578 Ps_contig_mira-and-tgicl-ass_franssen_22514
SEQUENCE=GTCGGGGGGTACTAACTAACTATTTTGCTAGTAGCATCGAAGAGAACAACATCAAAGTGCAAGGAGTCTCAACGCCCTCATGGCCACCTCTGCTATCCAACAATCAGCTTTCACTGGTAAGACTGGYTTGAGGCAAGGGAAATGAATTCATCAAGGAAAGCGTAGGAAACGTTTGGACCAAGCTCGCTTACCNTGCGTCGTACTGTGAAGAGTCGCCTACC
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02579 Ps_contig_mira-and-tgicl-ass_franssen_22536
SEQUENCE=TGATGAGTATCAAAGGAGGGACAGTGTCCCTCTTGATCGTGCCATTGTTAAGGACACCACTGGTGACTATGAGAAAATGCTTTTGGCAATCTTAGGACATGATGATGCTTGAGATATATGTTGGTATGTTYGTGTAGTGTGTGTGTGTTTTCCTTTTGAGAGAGGTACTATGTGGTTTATAAGCTGTTGTTTTCAGCATGGTCTTGTTTTTCCTTTGTGTTATTGTGTAGTTTTGGGGAGTTAATTAATTT
TARGET=137,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02580 Ps_contig_mira-and-tgicl-ass_franssen_22576
SEQUENCE=TTACNCCTACTTATTTTTTACTAATGTATCGTATGCACAACCAGCTTCAGAGATACACTTGATTCAGAGACACACAGAACAAGACACAAGAAAACCAGATCCAAGTAAAGAAACAACAGATCCATTACAGAACAAGTACCCAATTCTAGATCTAGATTGATCAATCAGTAGATCTCAACATCTAAGTAAATTTAAACTTTRAATAATCAATAAACGATAACCATACTAAAACNCTACCTCCCTCCTAGTTTCCCACCCGCCCGCCCGCCGCCT
TARGET=256,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02581 Ps_contig_mira-and-tgicl-ass_franssen_22578
SEQUENCE=GAGACTGGTGTTTGGTGGGAACTTCCTTAGGATCTGATGCGGGTGTTTGAGTATGAAACATTAGGGCTGATTACTTATTTGTGTACTAGCTAGAGTTAGGGTTCCATTGTTTCTCTTTCTCTGTTTCTTGTCAATTGGCCATACTTTCCCACTAGAGGAAAACGAAAACTACCACTTTGTWGAMWAWAYTATGATTATTGTTTTTATTTTTGTTGGTTGGTTGGTCTTGTAAATAGGCCGTCGTTTCTACTCCTCCA
TARGET=212,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02582 Ps_contig_mira-and-tgicl-ass_franssen_22591
SEQUENCE=CGGGGTTTTKAACTTACAAACCTAAGCATTTTCAACTATTTATATATATATTCACAGAGATCATATCGTATATCATAAGATGACTGGTTTTTCCAGCAACATCTTTGGTGGGTACGATGGTGGAAACAATGGAACTAGTTGATGCATTTAACAACAAGTGTGGCTATACAAGATTATGGTGAGGCTGACATCAAAACTGGTGCACAAATTACCATGGNGGTTACACTAAT
TARGET=41,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02583 Ps_contig_mira-and-tgicl-ass_franssen_22593
SEQUENCE=CCTAAACGTACGAAGAAGACGTATTGTAGAGGAAGACGAACAAGACACTTCGGGTACGAAAAGTAAGTAAGTAGCATCAGAAGACAGGACTAGGAGCTGGTGAATCGGCAGCTGAAACACCACTTGTAGCTATCACCAACAAAGCAACCATAACCGCCACAAAAACATTCATCTTCAGTGCTTCCATTGTTTTCAAGAGAGAGAATGTTTTGAAACAAAGAAAGATTAGTGGCAGTCCCCGCGTACT
TARGET=61,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0270
PRIMER_LEFT_PENALTY=0.931499
PRIMER_RIGHT_PENALTY=0.095538
PRIMER_LEFT_SEQUENCE=ACAAGACACTTCGGGTACGAAA
PRIMER_RIGHT_SEQUENCE=GTTATGGTTGCTTTGTTGGTGA
PRIMER_LEFT=39,22
PRIMER_RIGHT=153,22
PRIMER_LEFT_TM=60.931
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=1.1467
PRIMER_LEFT_1_PENALTY=0.931499
PRIMER_RIGHT_1_PENALTY=0.215215
PRIMER_LEFT_1_SEQUENCE=ACAAGACACTTCGGGTACGAAA
PRIMER_RIGHT_1_SEQUENCE=CTTGAAAACAATGGAAGCACTG
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=196,22
PRIMER_LEFT_1_TM=60.931
PRIMER_RIGHT_1_TM=59.785
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=1.1804
PRIMER_LEFT_2_PENALTY=0.931499
PRIMER_RIGHT_2_PENALTY=0.248900
PRIMER_LEFT_2_SEQUENCE=ACAAGACACTTCGGGTACGAAA
PRIMER_RIGHT_2_SEQUENCE=CAATGGAAGCACTGAAGATGAA
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=188,22
PRIMER_LEFT_2_TM=60.931
PRIMER_RIGHT_2_TM=60.249
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=1.1912
PRIMER_LEFT_3_PENALTY=0.931499
PRIMER_RIGHT_3_PENALTY=0.259704
PRIMER_LEFT_3_SEQUENCE=ACAAGACACTTCGGGTACGAAA
PRIMER_RIGHT_3_SEQUENCE=ACAATGGAAGCACTGAAGATGA
PRIMER_LEFT_3=39,22
PRIMER_RIGHT_3=189,22
PRIMER_LEFT_3_TM=60.931
PRIMER_RIGHT_3_TM=59.740
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=1.1912
PRIMER_LEFT_4_PENALTY=0.931499
PRIMER_RIGHT_4_PENALTY=0.259704
PRIMER_LEFT_4_SEQUENCE=ACAAGACACTTCGGGTACGAAA
PRIMER_RIGHT_4_SEQUENCE=TGGAAGCACTGAAGATGAATGT
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=60.931
PRIMER_RIGHT_4_TM=59.740
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_02584 Ps_contig_mira-and-tgicl-ass_franssen_22596
SEQUENCE=CAGTCTTCATGGTGGTTTGAACAAGCTTGCAGAGTTGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATTAGTTTACTCACTGCTTGTGCTATTTAAGAAAATTGGAGGGATTACGTTCTTCAATGGACCGAAAACAGAAGAAAATTACTTCGTTGGGTTGTTGTTGTAGTGGATTAGGTKGTKGAGACAGACTTAATTAGAAAAGAAAAGAAAATTATTAAGTTTGGGTTTAGTAAGTA
TARGET=213,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02585 Ps_contig_mira-and-tgicl-ass_franssen_22597
SEQUENCE=GAAACCTATCAATCAACACAAAAATGACCCCTTTCTTTCTCTCACTCATCTCTCTCTTTTTCCTTATCATCATCACATTGAAGCTCKTCTTCCAAAATGTGTGTGTTCTATGGGCTGTTTGTGTGTCTTATGTTGGTGTTTTGCGTGTCAATGACCACCTCTTCAAGGTTTGATGAACTCTTCCAACCAAGTTGGGCTTTTGACCTATTTTGTTCACGAAGGAGATCTCCTTAAACTCATCTCCTCCACCTCCACCAGTTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCCTACCCTCACCCACACCCAGTGTACCACTCTCCTCCACCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCT
TARGET=397,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02586 Ps_contig_mira-and-tgicl-ass_franssen_22599
SEQUENCE=ACAACCATGGAGGCGGAGGAGGATACAATCATGGCGGTGGTGGTGGTTATCGTGGTGGAGGTGGACATGGTGGTGTTTCCAACAATGGTAACTGATTATATCATG
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02587 Ps_contig_mira-and-tgicl-ass_franssen_22603
SEQUENCE=ACGTTTCTAATTTAATGTCTTCGAACATTGTTCAGACTTTGGGAATGATGCTCGACACAGTTGTGTTCTAGAGTTAGGATCGTCCACTGTATTCTTCTTCTTCACGATGTCCAGCAATAATTTCATTTACACTCTTTGACCTGTTTGCAATTAGATTTGTTTTTTTATTTACAAAGTTATTTGCATTTCATTGTGTTCGTCTCAAATGTTTCCGGATGATAATCATTTTCTTAAGCTTTAGAGACACGTGGGGACTCTGCTACAAAATCATAGAATGCTATTTGTAATTTTTAAAGTAAAAAAAACC
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0336
PRIMER_LEFT_PENALTY=0.029253
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=TGTTCAGACTTTGGGAATGATG
PRIMER_RIGHT_SEQUENCE=GATTTTGTAGCAGAGTCCCCAC
PRIMER_LEFT=28,22
PRIMER_RIGHT=268,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=0.0445
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.015253
PRIMER_LEFT_1_SEQUENCE=TGTTCAGACTTTGGGAATGATG
PRIMER_RIGHT_1_SEQUENCE=GGAAACATTTGAGACGAACACA
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=212,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=60.015
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.1963
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.167063
PRIMER_LEFT_2_SEQUENCE=TGTTCAGACTTTGGGAATGATG
PRIMER_RIGHT_2_SEQUENCE=AGAGTCCCCACGTGTCTCTAAA
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=60.167
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.2100
PRIMER_LEFT_3_PENALTY=0.029253
PRIMER_RIGHT_3_PENALTY=0.180765
PRIMER_LEFT_3_SEQUENCE=TGTTCAGACTTTGGGAATGATG
PRIMER_RIGHT_3_SEQUENCE=TTGCAAACAGGTCAAAGAGTGT
PRIMER_LEFT_3=28,22
PRIMER_RIGHT_3=149,22
PRIMER_LEFT_3_TM=59.971
PRIMER_RIGHT_3_TM=59.819
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=0.5335
PRIMER_LEFT_4_PENALTY=0.529160
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=CATTGTTCAGACTTTGGGAATG
PRIMER_RIGHT_4_SEQUENCE=GATTTTGTAGCAGAGTCCCCAC
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=268,22
PRIMER_LEFT_4_TM=59.471
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=244
=
PRIMER_SEQUENCE_ID=SSR_02588 Ps_contig_mira-and-tgicl-ass_franssen_22620
SEQUENCE=GAACAACAAAACCTATTTTGAGAAAACATGGGGTCTCAAATGACCTCTATTACTCTCACTATTGCATTGACAATACTCTCTCTCACCTTGCCATCTCAAGCAAAACTAATTTACCCTCTATTCATCCTCCACCACCACCAACCAAAACCTTCACTACCTAWCCWATCCTACCCACCCACCACCGGTNCACTCACCTCCTCCACCTTANCATTATGTTCCACCACCCACCACCAGT
TARGET=129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02589 Ps_contig_mira-and-tgicl-ass_franssen_22627
SEQUENCE=TGCCAAAAACCAATTAAACCAAAACCAAACTTTGTGGGGGTTTTATTTTTAATTTTGTAAATTCGATCATTATCTTGTTTTAACTATTTCCCTTTTTGTATGTAAAGGATGTTGTTGTAAGTGTAACGTGTTGTTGTAATGATTATTATTATTAGGTGGTGNGTGCGTTGGGTGNGTGGGCTGTTTTNATTACTTTAGTGATCNGCC
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02590 Ps_contig_mira-and-tgicl-ass_franssen_22632
SEQUENCE=CGGGGGTTAKTCGKKSTACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCAACCCCCAAACATGGAACAACCCTCCACTCTATCTTTCATCTCTCTACAAACCAACCAAGTTCACACCAAGAACAACAAAAMTTGCTGCACAAGAAGACAAACCGATGCANCAACCGATGTAGACAACAAAAGAAG
TARGET=22,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02591 Ps_contig_mira-and-tgicl-ass_franssen_22655
SEQUENCE=AGTAGGAGCAGCAGCATCACCTTTTGTGTAATATTTCACACTCAGCTTTGCAATGGATCCTCCATTTGGTCCTGCAGACAGTTTAGTCCTCAAATGAGATCTTCTCAACTGTGTCTGGAAATACCAAACAACCAACCAACTATGCTATAGTTGTAAGCCAAGTTAGCAACATCTAACTAACTCCATTTGNGCAACACAGTTTGGTTTTTCGGGTTTTCCAAACCCTATTCCTTTACCAAAACGGTAAAGGTGTAGTTTCTGATTGGTTCCA
TARGET=129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4636
PRIMER_LEFT_PENALTY=0.174518
PRIMER_RIGHT_PENALTY=0.289100
PRIMER_LEFT_SEQUENCE=GGTCCTGCAGACAGTTTAGTCC
PRIMER_RIGHT_SEQUENCE=TGGTAAAGGAATAGGGTTTGGA
PRIMER_LEFT=67,22
PRIMER_RIGHT=237,22
PRIMER_LEFT_TM=60.175
PRIMER_RIGHT_TM=59.711
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=0.4764
PRIMER_LEFT_1_PENALTY=0.187326
PRIMER_RIGHT_1_PENALTY=0.289100
PRIMER_LEFT_1_SEQUENCE=AGCAGCATCACCTTTTGTGTAA
PRIMER_RIGHT_1_SEQUENCE=TGGTAAAGGAATAGGGTTTGGA
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=237,22
PRIMER_LEFT_1_TM=59.813
PRIMER_RIGHT_1_TM=59.711
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=229
PRIMER_PAIR_PENALTY_2=0.6416
PRIMER_LEFT_2_PENALTY=0.174518
PRIMER_RIGHT_2_PENALTY=0.467116
PRIMER_LEFT_2_SEQUENCE=GGTCCTGCAGACAGTTTAGTCC
PRIMER_RIGHT_2_SEQUENCE=TGTGTTGCNCAAATGGAGTTAG
PRIMER_LEFT_2=67,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=60.175
PRIMER_RIGHT_2_TM=60.467
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.50
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=131
PRIMER_PAIR_PENALTY_3=0.6416
PRIMER_LEFT_3_PENALTY=0.174518
PRIMER_RIGHT_3_PENALTY=0.467116
PRIMER_LEFT_3_SEQUENCE=GGTCCTGCAGACAGTTTAGTCC
PRIMER_RIGHT_3_SEQUENCE=CTGTGTTGCNCAAATGGAGTTA
PRIMER_LEFT_3=67,22
PRIMER_RIGHT_3=198,22
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=60.467
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.50
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=0.7508
PRIMER_LEFT_4_PENALTY=0.461740
PRIMER_RIGHT_4_PENALTY=0.289100
PRIMER_LEFT_4_SEQUENCE=CCTGCAGACAGTTTAGTCCTCA
PRIMER_RIGHT_4_SEQUENCE=TGGTAAAGGAATAGGGTTTGGA
PRIMER_LEFT_4=70,22
PRIMER_RIGHT_4=237,22
PRIMER_LEFT_4_TM=59.538
PRIMER_RIGHT_4_TM=59.711
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=168
=
PRIMER_SEQUENCE_ID=SSR_02592 Ps_contig_mira-and-tgicl-ass_franssen_22660
SEQUENCE=GTCGGGGTAACACAAACCTAAGCATTTCAACTATTTATATATATATTCACAGAGATCATATCGTATATCATAAGATGTACTKGKTTTATTCAGACAAACATTCKTTKGGTAGGGTACGATGGTAGGAAACAATGGAACTGTTGATGACTAATTTAAACAACAAGTGTGG
TARGET=36,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02593 Ps_contig_mira-and-tgicl-ass_franssen_22664
SEQUENCE=GGATGGTATATCAGGTCACAGCAGTTGACAGTGGCAGTAGCAGTGATGAATGTGACAGCGACAACGAAACCGTCGCCACAGGAAGTACTGCAACTGAGAAGCTGCTGCATAATAATAATAAGTTGGTAGTTTGGAGGTATTAGTAAGTTTGGTGGTAGTATTGSWMGKTAAGGGTAAAGTTTAGAAAATTAAAGTTACGGTAACCGTTTAAAACGGTTTAAAAAGTTAAACTTAAAACGTTGGTAAACGAAAAACTAAAAAAATTACTTAAACTTAAAAAAGTTAAAAAAGTTACTTTAAAAACTTTT
TARGET=109,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02594 Ps_contig_mira-and-tgicl-ass_franssen_22713
SEQUENCE=TTATCTACAAATACTTGTGTAATGATCTTTGTACTCCATGTACTGTCATTTCTTATCCCTTGAACTATAATTTAATTTCAAAGGAAATTTTCTACTTTGTTGGTTTCCAATTCCATTAAACATTTTCTTCTTTATGATTCAGGTTATTGGTGTAGAGCCTCTTGAAAGCAACATACTCTCAGGTGGAAAGCCAGGACCTCACAAAATTTCAGGGTATTGGGGCTGGTTTTGTGCCCGGGAATTTGGATGAAGAAGTGGTTGATGAAGTTATAGCGATATCTAGTGATGTAGCTATTGAAACTACAAAGCAAATAGCACTACAAAGAAGGCTTGCTGGTGGGAATTTCTTCAGGTGCTGCTGCTGCTGCAGCATTGCGAGGGTGGACTAAGACGAACCGTAAGGAAAAAGTAAGGGAAATCTTTAGTTGGTCGGTT
TARGET=354,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3764
PRIMER_LEFT_PENALTY=0.029253
PRIMER_RIGHT_PENALTY=0.347129
PRIMER_LEFT_SEQUENCE=TTGGATGAAGAAGTGGTTGATG
PRIMER_RIGHT_SEQUENCE=TCCTTACGGTTCGTCTTAGTCC
PRIMER_LEFT=242,22
PRIMER_RIGHT=403,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=59.653
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=162
PRIMER_PAIR_PENALTY_1=0.4338
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.404566
PRIMER_LEFT_1_SEQUENCE=TTGGATGAAGAAGTGGTTGATG
PRIMER_RIGHT_1_SEQUENCE=TTACGGTTCGTCTTAGTCCACC
PRIMER_LEFT_1=242,22
PRIMER_RIGHT_1=400,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=60.405
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.5542
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.524968
PRIMER_LEFT_2_SEQUENCE=TTGGATGAAGAAGTGGTTGATG
PRIMER_RIGHT_2_SEQUENCE=CCTTACGGTTCGTCTTAGTCCA
PRIMER_LEFT_2=242,22
PRIMER_RIGHT_2=402,22
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=60.525
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=0.5741
PRIMER_LEFT_3_PENALTY=0.226971
PRIMER_RIGHT_3_PENALTY=0.347129
PRIMER_LEFT_3_SEQUENCE=ATACTCTCAGGTGGAAAGCCAG
PRIMER_RIGHT_3_SEQUENCE=TCCTTACGGTTCGTCTTAGTCC
PRIMER_LEFT_3=172,22
PRIMER_RIGHT_3=403,22
PRIMER_LEFT_3_TM=59.773
PRIMER_RIGHT_3_TM=59.653
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=232
PRIMER_PAIR_PENALTY_4=0.5997
PRIMER_LEFT_4_PENALTY=0.029253
PRIMER_RIGHT_4_PENALTY=0.570438
PRIMER_LEFT_4_SEQUENCE=TTGGATGAAGAAGTGGTTGATG
PRIMER_RIGHT_4_SEQUENCE=ACCGACCAACTAAAGATTTCCC
PRIMER_LEFT_4=242,22
PRIMER_RIGHT_4=433,22
PRIMER_LEFT_4_TM=59.971
PRIMER_RIGHT_4_TM=60.570
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_02595 Ps_contig_mira-and-tgicl-ass_franssen_22723
SEQUENCE=CTTTGGCAGATTCTGGAGTGTCAAATCTCTTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCTGAGACAGGGGATAGGCAAGGCACACAGGGGATAGG
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8198
PRIMER_LEFT_PENALTY=1.180219
PRIMER_RIGHT_PENALTY=0.639568
PRIMER_LEFT_SEQUENCE=CTTTGGCAGATTCTGGAGTGTC
PRIMER_RIGHT_SEQUENCE=CTGTCTCAGGGGGAGATGAGTA
PRIMER_LEFT=0,22
PRIMER_RIGHT=100,22
PRIMER_LEFT_TM=61.180
PRIMER_RIGHT_TM=60.640
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=1.8871
PRIMER_LEFT_1_PENALTY=1.247566
PRIMER_RIGHT_1_PENALTY=0.639568
PRIMER_LEFT_1_SEQUENCE=TTTGGCAGATTCTGGAGTGTC
PRIMER_RIGHT_1_SEQUENCE=CTGTCTCAGGGGGAGATGAGTA
PRIMER_LEFT_1=1,21
PRIMER_RIGHT_1=100,22
PRIMER_LEFT_1_TM=60.248
PRIMER_RIGHT_1_TM=60.640
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=2.2968
PRIMER_LEFT_2_PENALTY=1.657215
PRIMER_RIGHT_2_PENALTY=0.639568
PRIMER_LEFT_2_SEQUENCE=CTTTGGCAGATTCTGGAGTGT
PRIMER_RIGHT_2_SEQUENCE=CTGTCTCAGGGGGAGATGAGTA
PRIMER_LEFT_2=0,21
PRIMER_RIGHT_2=100,22
PRIMER_LEFT_2_TM=59.343
PRIMER_RIGHT_2_TM=60.640
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=101
PRIMER_PAIR_PENALTY_3=2.5163
PRIMER_LEFT_3_PENALTY=1.180219
PRIMER_RIGHT_3_PENALTY=1.336037
PRIMER_LEFT_3_SEQUENCE=CTTTGGCAGATTCTGGAGTGTC
PRIMER_RIGHT_3_SEQUENCE=TGTCTCAGGGGGAGATGAGTA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=99,21
PRIMER_LEFT_3_TM=61.180
PRIMER_RIGHT_3_TM=59.664
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=2.6509
PRIMER_LEFT_4_PENALTY=0.504940
PRIMER_RIGHT_4_PENALTY=2.145958
PRIMER_LEFT_4_SEQUENCE=GGCAGATTCTGGAGTGTCAAAT
PRIMER_RIGHT_4_SEQUENCE=CTTGCCTATCCCCTGTCTCAG
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=112,21
PRIMER_LEFT_4_TM=60.505
PRIMER_RIGHT_4_TM=61.146
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_02596 Ps_contig_mira-and-tgicl-ass_franssen_22737
SEQUENCE=TGTTTTTGGTAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTYGTGCATTTAGAAAATTGAGGGATAYGTTCTTCAATGGTACGAAAACAGAAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTATTAGAAAGAAAGAAAGTATATTGGTTGAAGATTACAGTATCTCTTATGTGTGTGATTCTTTTTTACTGTGTACACTT
TARGET=165,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02597 Ps_contig_mira-and-tgicl-ass_franssen_22795
SEQUENCE=GTACATCAATTAACTCCTAGATAACACACACACAACACAAAAAATGGGTAAAGATGTTGAGGTTCAAGAACAAGGTGCTGAATTCTCAGCTAAGGATTACCAAGATCCCACCACCAGCACCTTAYTTTTTGATTCAGCTWGTAAAGTTTAAAAATGGTCTCTGTACAGAGTCTTGTTATAGCAKWAGKTTGTA
TARGET=24,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02598 Ps_contig_mira-and-tgicl-ass_franssen_22797
SEQUENCE=GATCATCCAAACAACAACAACACTATCATCATCGTCTCATACTTTACCTTCCATTTCAGAGTTACAGCTACATAACACCACCACGCGTGGAATCTCTTTCCATGACCAATCTCGCCATGTCTCCAGATCCCTCT
TARGET=10,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02599 Ps_contig_mira-and-tgicl-ass_franssen_22799
SEQUENCE=ATGGNTTCTACTGCTTCTACACTTCTGCTTAGATCCATATATGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTGTAGTAATAGGCTGGTGGAGGTGGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGYGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATACTGGAGGTTTCTCTACCGGTGGTTTGTAGGTCGGAGGTTTCTCAACTGGTGGCTTGTAGACTGGAGGCTTCTCTACGGGTGGTTTGTAGGTTGGAGGCTTTTTCAACGGGAGGCTTGTAGGCTGGAGGCTTCTCTACCGGTGGCTTGTAAACGGGAGGTTTCTCTACTGGTGGTTTGTAAACTGGAGGCTTCTCAACCGGAGG
TARGET=128,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02600 Ps_contig_mira-and-tgicl-ass_franssen_22799
SEQUENCE=ATGGNTTCTACTGCTTCTACACTTCTGCTTAGATCCATATATGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTGTAGTAATAGGCTGGTGGAGGTGGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGYGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATACTGGAGGTTTCTCTACCGGTGGTTTGTAGGTCGGAGGTTTCTCAACTGGTGGCTTGTAGACTGGAGGCTTCTCTACGGGTGGTTTGTAGGTTGGAGGCTTTTTCAACGGGAGGCTTGTAGGCTGGAGGCTTCTCTACCGGTGGCTTGTAAACGGGAGGTTTCTCTACTGGTGGTTTGTAAACTGGAGGCTTCTCAACCGGAGG
TARGET=184,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02601 Ps_contig_mira-and-tgicl-ass_franssen_22799
SEQUENCE=ATGGNTTCTACTGCTTCTACACTTCTGCTTAGATCCATATATGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTGTAGTAATAGGCTGGTGGAGGTGGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGYGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATACTGGAGGTTTCTCTACCGGTGGTTTGTAGGTCGGAGGTTTCTCAACTGGTGGCTTGTAGACTGGAGGCTTCTCTACGGGTGGTTTGTAGGTTGGAGGCTTTTTCAACGGGAGGCTTGTAGGCTGGAGGCTTCTCTACCGGTGGCTTGTAAACGGGAGGTTTCTCTACTGGTGGTTTGTAAACTGGAGGCTTCTCAACCGGAGG
TARGET=232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02602 Ps_contig_mira-and-tgicl-ass_franssen_22799
SEQUENCE=ATGGNTTCTACTGCTTCTACACTTCTGCTTAGATCCATATATGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTGTAGTAATAGGCTGGTGGAGGTGGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGYGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATACTGGAGGTTTCTCTACCGGTGGTTTGTAGGTCGGAGGTTTCTCAACTGGTGGCTTGTAGACTGGAGGCTTCTCTACGGGTGGTTTGTAGGTTGGAGGCTTTTTCAACGGGAGGCTTGTAGGCTGGAGGCTTCTCTACCGGTGGCTTGTAAACGGGAGGTTTCTCTACTGGTGGTTTGTAAACTGGAGGCTTCTCAACCGGAGG
TARGET=286,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02603 Ps_contig_mira-and-tgicl-ass_franssen_22799
SEQUENCE=ATGGNTTCTACTGCTTCTACACTTCTGCTTAGATCCATATATGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTGTAGTAATAGGCTGGTGGAGGTGGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGYGGGGAGTGGTAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGTGGTGGGGAGTGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATACTGGAGGTTTCTCTACCGGTGGTTTGTAGGTCGGAGGTTTCTCAACTGGTGGCTTGTAGACTGGAGGCTTCTCTACGGGTGGTTTGTAGGTTGGAGGCTTTTTCAACGGGAGGCTTGTAGGCTGGAGGCTTCTCTACCGGTGGCTTGTAAACGGGAGGTTTCTCTACTGGTGGTTTGTAAACTGGAGGCTTCTCAACCGGAGG
TARGET=337,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02604 Ps_contig_mira-and-tgicl-ass_franssen_22823
SEQUENCE=TTCGTGGCAATGATGCTGAACATTTTTGGTTTGACAATTGTAATTAGGAGGAGGATATCCATTATTGAAATAGCCTTGGTAGGTTTGGTATTGAGGATGAGGCGGCGGGTAGGTTGTTGCATAACCAGGTGGCGGCGGCGGAGAGTAGCCTTCGTAACCAGGTGGCGGTGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTTGGAGGGATAGTGTTTTAAGATGGTTATCGTTTANGATTTATTTAAGTTTAATTCTAGATGTTGAGNTCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTTGTTNTTCTTTACNGGAAATTCCGTTT
TARGET=230,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2163
PRIMER_LEFT_PENALTY=0.080323
PRIMER_RIGHT_PENALTY=0.136017
PRIMER_LEFT_SEQUENCE=TAGGTTGTTGCATAACCAGGTG
PRIMER_RIGHT_SEQUENCE=CACTATCCCTCCAAGTTTCCAC
PRIMER_LEFT=109,22
PRIMER_RIGHT=263,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=59.864
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.2163
PRIMER_LEFT_1_PENALTY=0.080323
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=TAGGTTGTTGCATAACCAGGTG
PRIMER_RIGHT_1_SEQUENCE=ACACTATCCCTCCAAGTTTCCA
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=264,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.3211
PRIMER_LEFT_2_PENALTY=0.185055
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=GAGTAGCCTTCGTAACCAGGTG
PRIMER_RIGHT_2_SEQUENCE=CACTATCCCTCCAAGTTTCCAC
PRIMER_LEFT_2=142,22
PRIMER_RIGHT_2=263,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=0.3211
PRIMER_LEFT_3_PENALTY=0.185055
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=GAGTAGCCTTCGTAACCAGGTG
PRIMER_RIGHT_3_SEQUENCE=ACACTATCCCTCCAAGTTTCCA
PRIMER_LEFT_3=142,22
PRIMER_RIGHT_3=264,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=123
PRIMER_PAIR_PENALTY_4=0.3727
PRIMER_LEFT_4_PENALTY=0.236697
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=GCCTTGGTAGGTTTGGTATTGA
PRIMER_RIGHT_4_SEQUENCE=CACTATCCCTCCAAGTTTCCAC
PRIMER_LEFT_4=72,22
PRIMER_RIGHT_4=263,22
PRIMER_LEFT_4_TM=60.237
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_02605 Ps_contig_mira-and-tgicl-ass_franssen_22831
SEQUENCE=CCATACAGAAGCACTGTGGACTTGTTTTTTTTCTAAGACCATCCCACTTCTTCCTTCAAATCGGTAAACAACTCATTGGTATCGAGAGAAGTAGATGTATCATCAGAAGAAGAAGATGATAATGTCAATTGAGG
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02606 Ps_contig_mira-and-tgicl-ass_franssen_22878
SEQUENCE=GTAYCGATTAATAATTATTTTTATTCCATATGATAAAGTGCATAACTAATACGTTATGCACGATATTTATTACACACATATTTATGATTCAAAGAAGACGCAAACTCATATACATAGATAGATGCAGTGCTAGCTACTACAGCATGCTTATTTTTATTTTATTTATTTATATTATGCGTTCTATTCTTCTTTGTGTTGGAGAATCAATCCACTCCCTTAACCG
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02607 Ps_contig_mira-and-tgicl-ass_franssen_22887
SEQUENCE=TCGGGGGCCGATAACAAATAGCGCCGTCGCAATGGGAAGAAGACCTGCAAGATGTTACCGTCAAATTAAAAACAAGCCATATCCAAAGTCACGATTCTGTCGTGGTGTTCCTGATCCCAAGATACAGGACCTACGATGTTGGCATGAAGAAGCAGGGATCGTGGTTANGGAGGAAGTGGTGGAGGNGGTGTTATCNGNGGAGGCGGCGGCGGCGGTAGGAAACTGGGAGGGATAGTGTTTAG
TARGET=202,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3004
PRIMER_LEFT_PENALTY=0.060824
PRIMER_RIGHT_PENALTY=0.239567
PRIMER_LEFT_SEQUENCE=AAGACCTGCAAGATGTTACCGT
PRIMER_RIGHT_SEQUENCE=ACACTATCCCTCCCAGTTTCCT
PRIMER_LEFT=39,22
PRIMER_RIGHT=237,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=60.240
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.3771
PRIMER_LEFT_1_PENALTY=0.137550
PRIMER_RIGHT_1_PENALTY=0.239567
PRIMER_LEFT_1_SEQUENCE=AGCCATATCCAAAGTCACGATT
PRIMER_RIGHT_1_SEQUENCE=ACACTATCCCTCCCAGTTTCCT
PRIMER_LEFT_1=74,22
PRIMER_RIGHT_1=237,22
PRIMER_LEFT_1_TM=59.862
PRIMER_RIGHT_1_TM=60.240
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.3771
PRIMER_LEFT_2_PENALTY=0.137550
PRIMER_RIGHT_2_PENALTY=0.239567
PRIMER_LEFT_2_SEQUENCE=AAGCCATATCCAAAGTCACGAT
PRIMER_RIGHT_2_SEQUENCE=ACACTATCCCTCCCAGTTTCCT
PRIMER_LEFT_2=73,22
PRIMER_RIGHT_2=237,22
PRIMER_LEFT_2_TM=59.862
PRIMER_RIGHT_2_TM=60.240
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.4709
PRIMER_LEFT_3_PENALTY=0.231323
PRIMER_RIGHT_3_PENALTY=0.239567
PRIMER_LEFT_3_SEQUENCE=CTACGATGTTGGCATGAAGAAG
PRIMER_RIGHT_3_SEQUENCE=ACACTATCCCTCCCAGTTTCCT
PRIMER_LEFT_3=130,22
PRIMER_RIGHT_3=237,22
PRIMER_LEFT_3_TM=59.769
PRIMER_RIGHT_3_TM=60.240
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=108
PRIMER_PAIR_PENALTY_4=0.5092
PRIMER_LEFT_4_PENALTY=0.269585
PRIMER_RIGHT_4_PENALTY=0.239567
PRIMER_LEFT_4_SEQUENCE=ATACAGGACCTACGATGTTGGC
PRIMER_RIGHT_4_SEQUENCE=ACACTATCCCTCCCAGTTTCCT
PRIMER_LEFT_4=121,22
PRIMER_RIGHT_4=237,22
PRIMER_LEFT_4_TM=60.270
PRIMER_RIGHT_4_TM=60.240
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=117
=
PRIMER_SEQUENCE_ID=SSR_02608 Ps_contig_mira-and-tgicl-ass_franssen_22899
SEQUENCE=TAATATCGAGTTTGAGGAATGAGGATATTTTTTTGTATAAAAATTTTGAAAAAAAAAAAGTTTTTTTTTAGTTTTGTTTTGAAGTTAAAAAGTTGAATTCTAATTATTATTACTATCGTTATATGTGATGATGATGAACATGATGATATGTAATGTTTGGGGTTTTATGCTGTACTCTATTATGGGATATCACAGGTGATTGTTGATATCGTGAATTGTAATTTCAATGCAACAAAGAATWAATTTAATWRGTCKTAWWAGGTTAAATTAAAAAAGT
TARGET=126,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02609 Ps_contig_mira-and-tgicl-ass_franssen_22915
SEQUENCE=TTTTGAGACCAAAAGAGAATATCATTATTTTTATAATTTAAAGTTCACAATTACAACCATACATCACACATGGGCTGGGCCAGATTCCAAACTTAAAAGTAATGAAAAAACTGCCCCATCACCCAACGCACTCACCACCTAATAATAATAATCATTACAACAACACGTTACACTTACAACAACATCCTTTACAATAACAAAAAGGGAATAAGTTAAAACAAGATAATGATCGAATTTACAAAATTAAAAACAAACCCCANAAGTTTGGTTTAGKTATGTTGNNCAACTATCACAACGTTGATCATATCAACTTGACTTAAGG
TARGET=140,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02610 Ps_contig_mira-and-tgicl-ass_franssen_22942
SEQUENCE=GATGAGAAGAACAACGAGTTTGCCTTATAATTTCTCTTGGKCTATCCAGCAACGTGAAAAAGCTCACCCATGGCAACTGCTTCGTTGGTCTCTGCTGCTCTAAAAAGGTAATCATGCCGCAACTTCACTTCGTTTAATTCGTATATACGATAACGAGTTTCTTCACACTTAACTAACCAATAGTTTTGGGTACATATCATATATATATATGAAGGCTCGAGGGAAAGGTGG
TARGET=199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02611 Ps_contig_mira-and-tgicl-ass_franssen_22967
SEQUENCE=CGTTCAAAGGTACGTCTCCTGGGACGAAGACTCTGGAACATCATCATCATTCTCTTCTTGTTGCTGTGGCTGTGCCAGCACCCGCTTCAGGAACCTGCTTATTGAACTGCTCAGCTATCTTCTTCAGGTTTTCCAAATTATCCGGCCCTAATTGGTGGATAATGCTAGGAAGTATGTCTTGCAACTTCTTGGTTTGAGGAGCACCACTAACAACCCATGTATTAGCTGCAATGGATGCTTGAACTTTAGGGTTTGAGAACTGGATCGACCACATCATCCTTAAAGAATATTGACCTCCTCAATTTGAGGGATGGCATTTCCACCCCCAAACTTTCTTCTTCAGAGTACTCTGAAGCCTTTTGTTCGG
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2184
PRIMER_LEFT_PENALTY=1.217702
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=CGTTCAAAGGTACGTCTCCTG
PRIMER_RIGHT_SEQUENCE=AACCCTAAAGTTCAAGCATCCA
PRIMER_LEFT=0,21
PRIMER_RIGHT=252,22
PRIMER_LEFT_TM=59.782
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=1.4229
PRIMER_LEFT_1_PENALTY=1.217702
PRIMER_RIGHT_1_PENALTY=0.205226
PRIMER_LEFT_1_SEQUENCE=CGTTCAAAGGTACGTCTCCTG
PRIMER_RIGHT_1_SEQUENCE=GTGCTCCTCAAACCAAGAAGTT
PRIMER_LEFT_1=0,21
PRIMER_RIGHT_1=203,22
PRIMER_LEFT_1_TM=59.782
PRIMER_RIGHT_1_TM=59.795
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=1.4286
PRIMER_LEFT_2_PENALTY=1.217702
PRIMER_RIGHT_2_PENALTY=0.210886
PRIMER_LEFT_2_SEQUENCE=CGTTCAAAGGTACGTCTCCTG
PRIMER_RIGHT_2_SEQUENCE=AGCAGTTCAATAAGCAGGTTCC
PRIMER_LEFT_2=0,21
PRIMER_RIGHT_2=110,22
PRIMER_LEFT_2_TM=59.782
PRIMER_RIGHT_2_TM=59.789
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=1.4483
PRIMER_LEFT_3_PENALTY=1.217702
PRIMER_RIGHT_3_PENALTY=0.230621
PRIMER_LEFT_3_SEQUENCE=CGTTCAAAGGTACGTCTCCTG
PRIMER_RIGHT_3_SEQUENCE=AGCATCCATTGCAGCTAATACA
PRIMER_LEFT_3=0,21
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=59.782
PRIMER_RIGHT_3_TM=59.769
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=1.4637
PRIMER_LEFT_4_PENALTY=1.217702
PRIMER_RIGHT_4_PENALTY=0.245968
PRIMER_LEFT_4_SEQUENCE=CGTTCAAAGGTACGTCTCCTG
PRIMER_RIGHT_4_SEQUENCE=CAAGCATCCATTGCAGCTAATA
PRIMER_LEFT_4=0,21
PRIMER_RIGHT_4=240,22
PRIMER_LEFT_4_TM=59.782
PRIMER_RIGHT_4_TM=60.246
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_02612 Ps_contig_mira-and-tgicl-ass_franssen_22973
SEQUENCE=GTTCTTCGCTATTGATCGGGACAACATTCATTCATTCACCATGGCAATGGCAACACAAGYCTCTCTCTTCACACCACCACTCTCAACCCCCAAACATGGAAACAACCCTCCACTCTATCTTTCATCTCTCTCAAACCAACCAAGTTCACACCAAGAACAACAAAAAATTWGCTGCAACAGAAGACAAACCGATGCAGACAACCGATGTAGACAAACAAAAGAACGCACCGTAGTTGGTACTTACCCACCCCTAACCG
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02613 Ps_contig_mira-and-tgicl-ass_franssen_22979
SEQUENCE=TTGCTACTCCCCTGCGTGTCTCATGTCTATCATAAACGTTAATGTTTCTTCAAAGTAAGAAATAAACTTGAACAATTGATCTGAACAAGTTCCGTGAGTATACCATTGATTAACCCATAATTGAATGTTAGCATCTTCTTGATGATCCTTTTTAAATAACGCCGGCCAATTTCATTGAGTCTTGTCTTTATAGGAACAAAATCGTTCACATTTATGCCTTCTTTGTTGCAATCTCGTATCTCAACACCTTCTTTACTTGGCCATAACCCATGGATAGTAAATTTTTTGTTGGCAATGGAGAAACACAATCATTATTTTTTAAGTCTTGCAGTATGTTGGTGGCCATGTCTGAGACAGTTTAAAATGGTCGAATAAAAGAGGTGGTGGTGGTGGTGGTGGACGAGGAGGTGGTGGAGATCTTATCCTAGGTGGTGGAGGAGGAGACTT
TARGET=380,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1093
PRIMER_LEFT_PENALTY=0.064372
PRIMER_RIGHT_PENALTY=0.044904
PRIMER_LEFT_SEQUENCE=GGCCATAACCCATGGATAGTAA
PRIMER_RIGHT_SEQUENCE=TCCTCCACCACCTAGGATAAGA
PRIMER_LEFT=258,22
PRIMER_RIGHT=438,22
PRIMER_LEFT_TM=59.936
PRIMER_RIGHT_TM=59.955
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.1847
PRIMER_LEFT_1_PENALTY=0.139753
PRIMER_RIGHT_1_PENALTY=0.044904
PRIMER_LEFT_1_SEQUENCE=TGCAATCTCGTATCTCAACACC
PRIMER_RIGHT_1_SEQUENCE=TCCTCCACCACCTAGGATAAGA
PRIMER_LEFT_1=226,22
PRIMER_RIGHT_1=438,22
PRIMER_LEFT_1_TM=60.140
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.3079
PRIMER_LEFT_2_PENALTY=0.263023
PRIMER_RIGHT_2_PENALTY=0.044904
PRIMER_LEFT_2_SEQUENCE=TCTTTACTTGGCCATAACCCAT
PRIMER_RIGHT_2_SEQUENCE=TCCTCCACCACCTAGGATAAGA
PRIMER_LEFT_2=249,22
PRIMER_RIGHT_2=438,22
PRIMER_LEFT_2_TM=59.737
PRIMER_RIGHT_2_TM=59.955
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.6430
PRIMER_LEFT_3_PENALTY=0.598109
PRIMER_RIGHT_3_PENALTY=0.044904
PRIMER_LEFT_3_SEQUENCE=CCTTCTTTGTTGCAATCTCGTA
PRIMER_RIGHT_3_SEQUENCE=TCCTCCACCACCTAGGATAAGA
PRIMER_LEFT_3=216,22
PRIMER_RIGHT_3=438,22
PRIMER_LEFT_3_TM=59.402
PRIMER_RIGHT_3_TM=59.955
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.6659
PRIMER_LEFT_4_PENALTY=0.620981
PRIMER_RIGHT_4_PENALTY=0.044904
PRIMER_LEFT_4_SEQUENCE=ATGCCTTCTTTGTTGCAATCTC
PRIMER_RIGHT_4_SEQUENCE=TCCTCCACCACCTAGGATAAGA
PRIMER_LEFT_4=213,22
PRIMER_RIGHT_4=438,22
PRIMER_LEFT_4_TM=60.621
PRIMER_RIGHT_4_TM=59.955
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_02614 Ps_contig_mira-and-tgicl-ass_franssen_22990
SEQUENCE=ACGGTCATCATGGTGGTGGTTAACAATGGTTGGTGGAGGAGGTTACGGTCATGGTGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGCGGTGGACACTATGGTGGTGGACACTATGGAGGTGGACACTATGGTGGTGGTCACTACGGTCATGGTGGTGGTCATCATGGTGGACATGGTGGT
TARGET=52,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8203
PRIMER_LEFT_PENALTY=0.524309
PRIMER_RIGHT_PENALTY=0.295944
PRIMER_LEFT_SEQUENCE=ATCATGGTGGTGGTTAACAATG
PRIMER_RIGHT_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT=6,22
PRIMER_RIGHT=178,22
PRIMER_LEFT_TM=59.476
PRIMER_RIGHT_TM=60.296
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=1.0881
PRIMER_LEFT_1_PENALTY=0.792166
PRIMER_RIGHT_1_PENALTY=0.295944
PRIMER_LEFT_1_SEQUENCE=GTGGTGGTTAACAATGGTTGGT
PRIMER_RIGHT_1_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_1=12,22
PRIMER_RIGHT_1=178,22
PRIMER_LEFT_1_TM=60.792
PRIMER_RIGHT_1_TM=60.296
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=1.2966
PRIMER_LEFT_2_PENALTY=1.000680
PRIMER_RIGHT_2_PENALTY=0.295944
PRIMER_LEFT_2_SEQUENCE=GTGGTGGTTAACAATGGTTGG
PRIMER_RIGHT_2_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_2=12,21
PRIMER_RIGHT_2=178,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.296
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=1.2966
PRIMER_LEFT_3_PENALTY=1.000680
PRIMER_RIGHT_3_PENALTY=0.295944
PRIMER_LEFT_3_SEQUENCE=TGGTGGTTAACAATGGTTGGT
PRIMER_RIGHT_3_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_3=13,21
PRIMER_RIGHT_3=178,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.296
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=1.3989
PRIMER_LEFT_4_PENALTY=1.102945
PRIMER_RIGHT_4_PENALTY=0.295944
PRIMER_LEFT_4_SEQUENCE=ATGGTTGGTGGAGGAGGTTAC
PRIMER_RIGHT_4_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_4=25,21
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=60.296
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_02615 Ps_contig_mira-and-tgicl-ass_franssen_23004
SEQUENCE=GGCGGGGATAGCTTGAACGTTTCTCTGTCTACATTTTGTTGTTCCTCTCTCTCTCTCTAACACCGAGTGAGTGGGCTACCTTTCCAGCTGTGGGTGGGACGGACCCAAAARGGAGCTCATTCTTCYCTCCTTCACCGGTTCTATCCACCCGACCTACTTTTCTTCACTGGATTGGCGTATTTCTTATTTGCTTCTTTTCTACGTTCGTTTCCGGTCTTCTTTGGCCTCCCTCCTCCTCCGACCGTCACCAACCCAAACCCGCCACATCCAAGCAGTCCG
TARGET=45,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02616 Ps_contig_mira-and-tgicl-ass_franssen_23024
SEQUENCE=TTCCGAAATCAAACTCATCATTACATATACATAGTGATTTGTAGAATTGTGGGAAGATAGATTAATACATAAACACAAAAGATTGCAGTCTCTCTATACCTCTTTTGTTCAACACTTACCATTATTTATTCATGAAATTTTATTAATCAGAACATTGATCTAATCAGTTACCATTGTTGTAACCACCTCCGCCATTATGGTAACCACCACCGTTGTTGTAACCGCCACCACCGTTATGGTATCCGCCACCGTTATTGTAACCCCCTCCACCATTATGATAACCGCCGCCATTATTGTAACCACCACCACCATTATGGTAACCACCGCCACGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAG
TARGET=299,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.2447
PRIMER_LEFT_PENALTY=0.947963
PRIMER_RIGHT_PENALTY=2.296722
PRIMER_LEFT_SEQUENCE=GTATCCGCCACCGTTATTGTAA
PRIMER_RIGHT_SEQUENCE=GTGGTGGAGGTTACGGTCAT
PRIMER_LEFT=238,22
PRIMER_RIGHT=368,20
PRIMER_LEFT_TM=60.948
PRIMER_RIGHT_TM=59.703
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=131
PRIMER_PAIR_PENALTY_1=3.5087
PRIMER_LEFT_1_PENALTY=1.211969
PRIMER_RIGHT_1_PENALTY=2.296722
PRIMER_LEFT_1_SEQUENCE=TATCCGCCACCGTTATTGTAA
PRIMER_RIGHT_1_SEQUENCE=GTGGTGGAGGTTACGGTCAT
PRIMER_LEFT_1=239,21
PRIMER_RIGHT_1=368,20
PRIMER_LEFT_1_TM=60.212
PRIMER_RIGHT_1_TM=59.703
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=3.5627
PRIMER_LEFT_2_PENALTY=1.265933
PRIMER_RIGHT_2_PENALTY=2.296722
PRIMER_LEFT_2_SEQUENCE=GTATCCGCCACCGTTATTGTA
PRIMER_RIGHT_2_SEQUENCE=GTGGTGGAGGTTACGGTCAT
PRIMER_LEFT_2=238,21
PRIMER_RIGHT_2=368,20
PRIMER_LEFT_2_TM=59.734
PRIMER_RIGHT_2_TM=59.703
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=131
PRIMER_PAIR_PENALTY_3=3.5824
PRIMER_LEFT_3_PENALTY=0.947963
PRIMER_RIGHT_3_PENALTY=2.634484
PRIMER_LEFT_3_SEQUENCE=GTATCCGCCACCGTTATTGTAA
PRIMER_RIGHT_3_SEQUENCE=GGTCATGGTGGTGGAGGTTA
PRIMER_LEFT_3=238,22
PRIMER_RIGHT_3=354,20
PRIMER_LEFT_3_TM=60.948
PRIMER_RIGHT_3_TM=60.634
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=117
PRIMER_PAIR_PENALTY_4=3.5824
PRIMER_LEFT_4_PENALTY=0.947963
PRIMER_RIGHT_4_PENALTY=2.634484
PRIMER_LEFT_4_SEQUENCE=GTATCCGCCACCGTTATTGTAA
PRIMER_RIGHT_4_SEQUENCE=GGTCATGGTGGTGGAGGTTA
PRIMER_LEFT_4=238,22
PRIMER_RIGHT_4=375,20
PRIMER_LEFT_4_TM=60.948
PRIMER_RIGHT_4_TM=60.634
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_02617 Ps_contig_mira-and-tgicl-ass_franssen_23030
SEQUENCE=ACGGYGTGARTKAWRTACCTTATTTATTATCTATATATAGTTAATTTTATTTTTTCTTTAAGTTAATAGCTTATGCTTATAATAGTTGTTTATTTTATTCAACCTATTTYYTTGTATTATTTCTTCTTCTTCTTCTTTCAATTCATTCCCTATATGTAATTGAATTCCTTGTATCAATGTGGAGCAATAAATTCGTGAGTATATGATTTTGTTTCTCTAAAAGAAAACTGAAACACGCAACAGGGGATAGGCAAGGCACACAGGGGATAGG
TARGET=121,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02618 Ps_contig_mira-and-tgicl-ass_franssen_23033
SEQUENCE=CATCAGAAGAAGAAGCTCTAGTCCGATGAGAGAAAACTTCCGCGACTCTAAAGAAAAAAAATGTTTAGGGAAAGGGGAAGAAGAAGGAGTGGTGGTGGAGAGGCGAGGAGGAGGGAGATAAGGCAAAGCAGAGCAGCGAGTGGTGGTGGTCAGTAGCCGAGGAAGAGGAGGGACTAACACTGTGCTTGTGCTTGCTGCCATTGCCATTAACTCTTGTTTCTCTCTCTCTCTCACTACCTTTTTTCTGTTATCTGTCTTTCTTTCGTTTTCCCCGCGTTA
TARGET=219,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2760
PRIMER_LEFT_PENALTY=0.017127
PRIMER_RIGHT_PENALTY=0.258908
PRIMER_LEFT_SEQUENCE=CTTCCGCGACTCTAAAGAAAAA
PRIMER_RIGHT_SEQUENCE=GGGAAAACGAAAGAAAGACAGA
PRIMER_LEFT=36,22
PRIMER_RIGHT=271,22
PRIMER_LEFT_TM=60.017
PRIMER_RIGHT_TM=59.741
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.2801
PRIMER_LEFT_1_PENALTY=0.021195
PRIMER_RIGHT_1_PENALTY=0.258908
PRIMER_LEFT_1_SEQUENCE=GAGAAAACTTCCGCGACTCTAA
PRIMER_RIGHT_1_SEQUENCE=GGGAAAACGAAAGAAAGACAGA
PRIMER_LEFT_1=29,22
PRIMER_RIGHT_1=271,22
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=59.741
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.3134
PRIMER_LEFT_2_PENALTY=0.054491
PRIMER_RIGHT_2_PENALTY=0.258908
PRIMER_LEFT_2_SEQUENCE=GTTTAGGGAAAGGGGAAGAAGA
PRIMER_RIGHT_2_SEQUENCE=GGGAAAACGAAAGAAAGACAGA
PRIMER_LEFT_2=62,22
PRIMER_RIGHT_2=271,22
PRIMER_LEFT_2_TM=59.946
PRIMER_RIGHT_2_TM=59.741
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.3218
PRIMER_LEFT_3_PENALTY=0.062929
PRIMER_RIGHT_3_PENALTY=0.258908
PRIMER_LEFT_3_SEQUENCE=TTAGGGAAAGGGGAAGAAGAAG
PRIMER_RIGHT_3_SEQUENCE=GGGAAAACGAAAGAAAGACAGA
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=271,22
PRIMER_LEFT_3_TM=60.063
PRIMER_RIGHT_3_TM=59.741
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.4319
PRIMER_LEFT_4_PENALTY=0.173020
PRIMER_RIGHT_4_PENALTY=0.258908
PRIMER_LEFT_4_SEQUENCE=ATGTTTAGGGAAAGGGGAAGAA
PRIMER_RIGHT_4_SEQUENCE=GGGAAAACGAAAGAAAGACAGA
PRIMER_LEFT_4=60,22
PRIMER_RIGHT_4=271,22
PRIMER_LEFT_4_TM=60.173
PRIMER_RIGHT_4_TM=59.741
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_02619 Ps_contig_mira-and-tgicl-ass_franssen_23048
SEQUENCE=TTCATACAAGAGAAACGCCTGATAGGAAAATAATTTTGAAGAGATAAGCCAAGAAACTGGGAAGTATGTTTATGGAGTTGATGACACATTGAAGGCTCTAGAGATGGGTGCTGTTGAAACACTTATTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGGGGATAGGTGTGCACTGGTGGTGGTGGAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGAGGAGGAGAGTGGTAGACTGGGTGTGGGTGAGGGTAGGGAATTCCAGTTATATGATTCTGGTGTGTTTACAGGAAGATGTGGAACAGCACTAGACCATGGTGTTGCAGCAGTTGGATACGGAACAGAAAACGGTAAAGNTTATTGGATAGTTAGGAATTCATGGGGTGG
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2870
PRIMER_LEFT_PENALTY=0.117281
PRIMER_RIGHT_PENALTY=0.169745
PRIMER_LEFT_SEQUENCE=TTCATACAAGAGAAACGCCTGA
PRIMER_RIGHT_SEQUENCE=CACCCAGTCTACCACTCTCCTC
PRIMER_LEFT=0,22
PRIMER_RIGHT=270,22
PRIMER_LEFT_TM=59.883
PRIMER_RIGHT_TM=60.170
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=59.091
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.2878
PRIMER_LEFT_1_PENALTY=0.117281
PRIMER_RIGHT_1_PENALTY=0.170538
PRIMER_LEFT_1_SEQUENCE=TTCATACAAGAGAAACGCCTGA
PRIMER_RIGHT_1_SEQUENCE=TAACTGGAATTCCCTACCCTCA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=59.883
PRIMER_RIGHT_1_TM=59.829
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.2944
PRIMER_LEFT_2_PENALTY=0.124655
PRIMER_RIGHT_2_PENALTY=0.169745
PRIMER_LEFT_2_SEQUENCE=TCTAGAGATGGGTGCTGTTGAA
PRIMER_RIGHT_2_SEQUENCE=CACCCAGTCTACCACTCTCCTC
PRIMER_LEFT_2=96,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=60.170
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=59.091
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=175
PRIMER_PAIR_PENALTY_3=0.2952
PRIMER_LEFT_3_PENALTY=0.124655
PRIMER_RIGHT_3_PENALTY=0.170538
PRIMER_LEFT_3_SEQUENCE=TCTAGAGATGGGTGCTGTTGAA
PRIMER_RIGHT_3_SEQUENCE=TAACTGGAATTCCCTACCCTCA
PRIMER_LEFT_3=96,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=59.829
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=0.3206
PRIMER_LEFT_4_PENALTY=0.117281
PRIMER_RIGHT_4_PENALTY=0.203343
PRIMER_LEFT_4_SEQUENCE=TTCATACAAGAGAAACGCCTGA
PRIMER_RIGHT_4_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=239,22
PRIMER_LEFT_4_TM=59.883
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_02620 Ps_contig_mira-and-tgicl-ass_franssen_23048
SEQUENCE=TTCATACAAGAGAAACGCCTGATAGGAAAATAATTTTGAAGAGATAAGCCAAGAAACTGGGAAGTATGTTTATGGAGTTGATGACACATTGAAGGCTCTAGAGATGGGTGCTGTTGAAACACTTATTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGGGGATAGGTGTGCACTGGTGGTGGTGGAGGAGATGGGTACTTGTAAGGCTTCTTGTGTGGTGTTGGTGGAGGAGGAGAGTGGTAGACTGGGTGTGGGTGAGGGTAGGGAATTCCAGTTATATGATTCTGGTGTGTTTACAGGAAGATGTGGAACAGCACTAGACCATGGTGTTGCAGCAGTTGGATACGGAACAGAAAACGGTAAAGNTTATTGGATAGTTAGGAATTCATGGGGTGG
TARGET=194,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2870
PRIMER_LEFT_PENALTY=0.117281
PRIMER_RIGHT_PENALTY=0.169745
PRIMER_LEFT_SEQUENCE=TTCATACAAGAGAAACGCCTGA
PRIMER_RIGHT_SEQUENCE=CACCCAGTCTACCACTCTCCTC
PRIMER_LEFT=0,22
PRIMER_RIGHT=270,22
PRIMER_LEFT_TM=59.883
PRIMER_RIGHT_TM=60.170
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=59.091
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.2878
PRIMER_LEFT_1_PENALTY=0.117281
PRIMER_RIGHT_1_PENALTY=0.170538
PRIMER_LEFT_1_SEQUENCE=TTCATACAAGAGAAACGCCTGA
PRIMER_RIGHT_1_SEQUENCE=TAACTGGAATTCCCTACCCTCA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=59.883
PRIMER_RIGHT_1_TM=59.829
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.2944
PRIMER_LEFT_2_PENALTY=0.124655
PRIMER_RIGHT_2_PENALTY=0.169745
PRIMER_LEFT_2_SEQUENCE=TCTAGAGATGGGTGCTGTTGAA
PRIMER_RIGHT_2_SEQUENCE=CACCCAGTCTACCACTCTCCTC
PRIMER_LEFT_2=96,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=60.170
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=59.091
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=175
PRIMER_PAIR_PENALTY_3=0.2952
PRIMER_LEFT_3_PENALTY=0.124655
PRIMER_RIGHT_3_PENALTY=0.170538
PRIMER_LEFT_3_SEQUENCE=TCTAGAGATGGGTGCTGTTGAA
PRIMER_RIGHT_3_SEQUENCE=TAACTGGAATTCCCTACCCTCA
PRIMER_LEFT_3=96,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=59.829
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=0.3150
PRIMER_LEFT_4_PENALTY=0.246726
PRIMER_RIGHT_4_PENALTY=0.068302
PRIMER_LEFT_4_SEQUENCE=AATGGTAGACAGGGTGAGGATG
PRIMER_RIGHT_4_SEQUENCE=CCACCCCATGAATTCCTAACTA
PRIMER_LEFT_4=156,22
PRIMER_RIGHT_4=415,22
PRIMER_LEFT_4_TM=60.247
PRIMER_RIGHT_4_TM=60.068
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=260
=
PRIMER_SEQUENCE_ID=SSR_02621 Ps_contig_mira-and-tgicl-ass_franssen_23057
SEQUENCE=TTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTACTTTGTGTACACTTCTACCAGAAAGATATGGTTGTTTGTTTACTTGTAACTATTTTCTATCATTCTTTGTCTTGTAATTAGTAACTAACAAAAGTCTTAGTCTTTGTTTTGTTAGTCTTGTAGTTTCTTTAGTAAATCTACTTCTAGTTTAGTTGTTTACAAACTAAATTAAATTAAATTTA
TARGET=24,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02622 Ps_contig_mira-and-tgicl-ass_franssen_23057
SEQUENCE=TTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTACTTTGTGTACACTTCTACCAGAAAGATATGGTTGTTTGTTTACTTGTAACTATTTTCTATCATTCTTTGTCTTGTAATTAGTAACTAACAAAAGTCTTAGTCTTTGTTTTGTTAGTCTTGTAGTTTCTTTAGTAAATCTACTTCTAGTTTAGTTGTTTACAAACTAAATTAAATTAAATTTA
TARGET=254,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02623 Ps_contig_mira-and-tgicl-ass_franssen_23070
SEQUENCE=ACCACCACCGCCATGACCGTAACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGACCGTAGCCTCCATGTCCATGACCATAGCCATGACCATAACCTCCACCGTAATATTTGGCATCATTTACTTCATTTGACTTTTCAGCAACCTCCTTGGCATTAGTGGAAGTCTCAGCTAATTCTCTAGCTGAAACCTCGGAAGGAAATAACAAGAACCATGGCCAATAGACCAAGGATGAGGAAAACGTTTCTGGAA
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02624 Ps_contig_mira-and-tgicl-ass_franssen_23083
SEQUENCE=TTCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGCTTGATGGGGTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGTGTGTNTATATTGCAATAGAAATTAGAAAAGTATTTGTTATGTCTTTTGAATTAATTGTTATTTCTTATACAAGTTCCGGGTCCCCCGCGTTACT
TARGET=129,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7255
PRIMER_LEFT_PENALTY=0.301926
PRIMER_RIGHT_PENALTY=0.423592
PRIMER_LEFT_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_SEQUENCE=TGCAATATANACACACGATGGC
PRIMER_LEFT=18,22
PRIMER_RIGHT=167,22
PRIMER_LEFT_TM=60.302
PRIMER_RIGHT_TM=60.424
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=150
PRIMER_PAIR_PENALTY_1=0.7255
PRIMER_LEFT_1_PENALTY=0.301926
PRIMER_RIGHT_1_PENALTY=0.423592
PRIMER_LEFT_1_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_1_SEQUENCE=TGCAATATANACACACGATGGC
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=167,22
PRIMER_LEFT_1_TM=60.302
PRIMER_RIGHT_1_TM=60.424
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.9297
PRIMER_LEFT_2_PENALTY=0.301926
PRIMER_RIGHT_2_PENALTY=0.627767
PRIMER_LEFT_2_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_2_SEQUENCE=GGACCCGGAACTTGTATAAGAA
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=236,22
PRIMER_LEFT_2_TM=60.302
PRIMER_RIGHT_2_TM=59.372
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.9297
PRIMER_LEFT_3_PENALTY=0.301926
PRIMER_RIGHT_3_PENALTY=0.627767
PRIMER_LEFT_3_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_3_SEQUENCE=GGACCCGGAACTTGTATAAGAA
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=236,22
PRIMER_LEFT_3_TM=60.302
PRIMER_RIGHT_3_TM=59.372
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=1.0126
PRIMER_LEFT_4_PENALTY=0.589035
PRIMER_RIGHT_4_PENALTY=0.423592
PRIMER_LEFT_4_SEQUENCE=GAGGAAGCTACTTTCTTGGTGG
PRIMER_RIGHT_4_SEQUENCE=TGCAATATANACACACGATGGC
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=167,22
PRIMER_LEFT_4_TM=59.411
PRIMER_RIGHT_4_TM=60.424
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_02625 Ps_contig_mira-and-tgicl-ass_franssen_23101
SEQUENCE=GGAAGATGGGAGGAAAATATTGATAGCAGAAGTCGCAAGGAAGAGAGGGTTCAGTTTACCTTTTCCCTTTCTTCAATCTTCAATCTTCATTTTTCATCTTCCTTCTTCTCTCCAACACAAACACAGTCTCTCTCTCTTTTCACCTATCGCGTCCCTCTTTCTCTCGGGCTCTCACTGCGTCAAAAACCACGAGAAGAGGAACAACATGCAGATCTTCGTCAAGACTTTAACCGGAAAAACCATCACTCTCGAGTTTGAGAAGCAAGCGAACACCATCGACAACGTCAAGGCCAAAGATCCAGGACAAGGAAGGTATCCCCTCCTGACCAGCAGTAGG
TARGET=127,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2816
PRIMER_LEFT_PENALTY=0.243544
PRIMER_RIGHT_PENALTY=0.038090
PRIMER_LEFT_SEQUENCE=GGGTTCAGTTTACCTTTTCCCT
PRIMER_RIGHT_SEQUENCE=ATGTTGTTCCTCTTCTCGTGGT
PRIMER_LEFT=46,22
PRIMER_RIGHT=206,22
PRIMER_LEFT_TM=59.756
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=0.2816
PRIMER_LEFT_1_PENALTY=0.243544
PRIMER_RIGHT_1_PENALTY=0.038090
PRIMER_LEFT_1_SEQUENCE=AGGGTTCAGTTTACCTTTTCCC
PRIMER_RIGHT_1_SEQUENCE=ATGTTGTTCCTCTTCTCGTGGT
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=206,22
PRIMER_LEFT_1_TM=59.756
PRIMER_RIGHT_1_TM=60.038
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.3617
PRIMER_LEFT_2_PENALTY=0.243544
PRIMER_RIGHT_2_PENALTY=0.118120
PRIMER_LEFT_2_SEQUENCE=GGGTTCAGTTTACCTTTTCCCT
PRIMER_RIGHT_2_SEQUENCE=TCTTGACGAAGATCTGCATGTT
PRIMER_LEFT_2=46,22
PRIMER_RIGHT_2=223,22
PRIMER_LEFT_2_TM=59.756
PRIMER_RIGHT_2_TM=59.882
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=0.3617
PRIMER_LEFT_3_PENALTY=0.243544
PRIMER_RIGHT_3_PENALTY=0.118120
PRIMER_LEFT_3_SEQUENCE=AGGGTTCAGTTTACCTTTTCCC
PRIMER_RIGHT_3_SEQUENCE=TCTTGACGAAGATCTGCATGTT
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=223,22
PRIMER_LEFT_3_TM=59.756
PRIMER_RIGHT_3_TM=59.882
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=179
PRIMER_PAIR_PENALTY_4=0.3721
PRIMER_LEFT_4_PENALTY=0.243544
PRIMER_RIGHT_4_PENALTY=0.128591
PRIMER_LEFT_4_SEQUENCE=GGGTTCAGTTTACCTTTTCCCT
PRIMER_RIGHT_4_SEQUENCE=AGAGTGATGGTTTTTCCGGTTA
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=248,22
PRIMER_LEFT_4_TM=59.756
PRIMER_RIGHT_4_TM=59.871
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_02626 Ps_contig_mira-and-tgicl-ass_franssen_23107
SEQUENCE=TTTATATATATATAAATATATAAATATATAAATATACTCTGCAAGCAGTSKTWACGCAGAGTATATTATATATTAATTAAATACGATAGATAATTAAATATATAAATATACTCTGCGTTGATACCA
TARGET=3,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02627 Ps_contig_mira-and-tgicl-ass_franssen_23123
SEQUENCE=GAGATATAAAATACTAGAAGCGAGGGTACGGAAGAGAAGAGAAGAAAATGAAAGAAAATGGATCTTAAGCAAATTATCCCACTCAACCGTGAACTGAAGAAGTAGAAGAAAGAGTTATCAGAATCAGCATGAACTGTCTCCACAATCTTTCAAGATTCTCTCTTTTACCCTTCCACACACTTGTTACTCCTCATTCTCCCACCACTCTTCTTCTCTATCCTTCATCCGTAATCT
TARGET=31,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0571
PRIMER_LEFT_PENALTY=1.051515
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=AAATACTAGAAGCGAGGGTACGG
PRIMER_RIGHT_SEQUENCE=AGTGGTGGGAGAATGAGGAGTA
PRIMER_LEFT=8,23
PRIMER_RIGHT=205,22
PRIMER_LEFT_TM=60.052
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=1.1385
PRIMER_LEFT_1_PENALTY=1.132937
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=AATACTAGAAGCGAGGGTACGG
PRIMER_RIGHT_1_SEQUENCE=AGTGGTGGGAGAATGAGGAGTA
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=58.867
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.9000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=1.1616
PRIMER_LEFT_2_PENALTY=1.051515
PRIMER_RIGHT_2_PENALTY=0.110131
PRIMER_LEFT_2_SEQUENCE=AAATACTAGAAGCGAGGGTACGG
PRIMER_RIGHT_2_SEQUENCE=GAAGAAGAGTGGTGGGAGAATG
PRIMER_LEFT_2=8,23
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=60.052
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=1.1616
PRIMER_LEFT_3_PENALTY=1.051515
PRIMER_RIGHT_3_PENALTY=0.110131
PRIMER_LEFT_3_SEQUENCE=AAATACTAGAAGCGAGGGTACGG
PRIMER_RIGHT_3_SEQUENCE=AAGAAGAGTGGTGGGAGAATGA
PRIMER_LEFT_3=8,23
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=60.052
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=1.1955
PRIMER_LEFT_4_PENALTY=1.051515
PRIMER_RIGHT_4_PENALTY=0.143960
PRIMER_LEFT_4_SEQUENCE=AAATACTAGAAGCGAGGGTACGG
PRIMER_RIGHT_4_SEQUENCE=GAGAAGAAGAGTGGTGGGAGAA
PRIMER_LEFT_4=8,23
PRIMER_RIGHT_4=214,22
PRIMER_LEFT_4_TM=60.052
PRIMER_RIGHT_4_TM=59.856
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_02628 Ps_contig_mira-and-tgicl-ass_franssen_23148
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGGTCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTGTGTAGWKTCTTTTTTTACTTGTGTACACTTCTAACCG
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02629 Ps_contig_mira-and-tgicl-ass_franssen_23157
SEQUENCE=TTACGTTCGGGGTTACCTTAATTATTCAGCATCATCATCATCATCACATTCAAGTTCAAGAAAAAAAGAAACTACTATGGYTCRRGSYATGGCATCATCAATGGCTGGTTGTTTACGTGGTTGTTCATCTCAAACTGTGTTAGAAGGAAGTCTTCAATTTAGTGGACCAAACAGGTTGAGTTTGTTGCACGGAACACCAACAACGTTAACAAGGTTACAACACGTTCATCAGTGACGGTTAGRGCTCAACANGGAGAGTAGTCGAAGGGCTGTGATTGGTCTTGTTGCTACTGGTTTGTTCTTCTTNTTTGTCAAG
TARGET=30,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02630 Ps_contig_mira-and-tgicl-ass_franssen_23163
SEQUENCE=CCTCGTTATTACGACCATTTGGTATGTTTGATTTTTGCTAATGAGAAAGTGATAAAGAAGAAGAAGAGAAAGAAGGGTAGTATGGTCATTGATGCCATGATTGTGTTTGGCTAAGAAGAGAAAGGATGGGGTTTAAAAGGTTTTTGGTGTCCCCCSCGGCGGTAACTCTGCGTTGATACCNTGCTT
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02631 Ps_contig_mira-and-tgicl-ass_franssen_23167
SEQUENCE=TTCGTACTTTACCTTTTACCTATTTCTAGAGTTACTAGCTACATAATCCATTTTAGTTTATTAGTTTAATTAATTTMCYAGTTCGATCCATCTTGTTAGTTAGTGATCATCATGGCTGAGGAGAATCAGAACAAGTACGAGGAGACTACATCCGCAACCAACTCTGAGACAGAGRTCAAGGACAGAGGTTGTTTTTGATTTTCTCGGTGGTAAGAAAAAGGATGAAGAACACAAGCCTGCTCAAGATGAGGCGATTGCCCACGAGTTTGGTCACAAGGTGACTTTGTATGAAGCACCGTCGGAAACCAAAGTTGAAGAGGAAGGTAGAAAAGAAACAACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCCTCCTACAAGKWGRAAGGAGGAAGGAGAAGATGGWGAAGAAARRAAGAAGAAGAAGAANAAGAAGAAAGAAGAANACTACGGTCGACGAGTA
TARGET=429,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02632 Ps_contig_mira-and-tgicl-ass_franssen_23177
SEQUENCE=TTCAGGATTCTCAACTGGAACTTCCTCTGTCTTGTCTGGAGCTTCCACTGATTCAACTGTTTTGTTCTCAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTTGCTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTTCACTTCTTCAACCGCTTCCTCAGTTTCTTTAGGTGCTTCTAAGTTCTCTTCCTTTGCTTCCTCTGTTACCACTTCCTTGTTCTCAACTTCAAG
TARGET=91,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5668
PRIMER_LEFT_PENALTY=0.564464
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=CTTCCTCTGTCTTGTCTGGAGC
PRIMER_RIGHT_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_LEFT=20,22
PRIMER_RIGHT=172,22
PRIMER_LEFT_TM=60.564
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.5668
PRIMER_LEFT_1_PENALTY=0.564464
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=TCCTCTGTCTTGTCTGGAGCTT
PRIMER_RIGHT_1_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=172,22
PRIMER_LEFT_1_TM=60.564
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=0.6451
PRIMER_LEFT_2_PENALTY=0.642812
PRIMER_RIGHT_2_PENALTY=0.002310
PRIMER_LEFT_2_SEQUENCE=CTCAATCACATCCTCTGCAGTT
PRIMER_RIGHT_2_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=172,22
PRIMER_LEFT_2_TM=59.357
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=107
PRIMER_PAIR_PENALTY_3=0.6877
PRIMER_LEFT_3_PENALTY=0.564464
PRIMER_RIGHT_3_PENALTY=0.123278
PRIMER_LEFT_3_SEQUENCE=TCCTCTGTCTTGTCTGGAGCTT
PRIMER_RIGHT_3_SEQUENCE=CAGAGGAAGCAAAGGAAGAGAA
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=226,22
PRIMER_LEFT_3_TM=60.564
PRIMER_RIGHT_3_TM=60.123
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=0.7290
PRIMER_LEFT_4_PENALTY=0.564464
PRIMER_RIGHT_4_PENALTY=0.164560
PRIMER_LEFT_4_SEQUENCE=TCCTCTGTCTTGTCTGGAGCTT
PRIMER_RIGHT_4_SEQUENCE=GTGGTAACAGAGGAAGCAAAGG
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=60.564
PRIMER_RIGHT_4_TM=60.165
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_02633 Ps_contig_mira-and-tgicl-ass_franssen_23194
SEQUENCE=CAAAGTAGAAATTATATCTTCCTGCATTTTGCATTATCAGTGGGATGGTCTTGAAAACAAGTCCACAGTGCTTCTGTATGGTGGTGGTGGTTTGGTTGCTGTTTGGTTATCTTCAATTCTTGTAGGTGCCATCAACTCAGTCCCTTGCTTCCAAAGATTTAGTGGGAGGTTGGGTAGGACTAGGGTACACTGGATGGTTTGTCTACCGGTACCTTCTGTTTAAGTCTAGAGAAAGGAACTAGCTACGGATATTGAGGCACTAGAAAAGAAATTGACCGG
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2188
PRIMER_LEFT_PENALTY=0.215225
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=GCATTATCAGTGGGATGGTCTT
PRIMER_RIGHT_SEQUENCE=TTGATGGCACCTACAAGAATTG
PRIMER_LEFT=30,22
PRIMER_RIGHT=134,22
PRIMER_LEFT_TM=60.215
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=105
PRIMER_PAIR_PENALTY_1=0.2895
PRIMER_LEFT_1_PENALTY=0.180765
PRIMER_RIGHT_1_PENALTY=0.108707
PRIMER_LEFT_1_SEQUENCE=TTGAAAACAAGTCCACAGTGCT
PRIMER_RIGHT_1_SEQUENCE=ACCCAACCTCCCACTAAATCTT
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=174,22
PRIMER_LEFT_1_TM=59.819
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=0.2895
PRIMER_LEFT_2_PENALTY=0.180765
PRIMER_RIGHT_2_PENALTY=0.108707
PRIMER_LEFT_2_SEQUENCE=TGAAAACAAGTCCACAGTGCTT
PRIMER_RIGHT_2_SEQUENCE=ACCCAACCTCCCACTAAATCTT
PRIMER_LEFT_2=51,22
PRIMER_RIGHT_2=174,22
PRIMER_LEFT_2_TM=59.819
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=0.2895
PRIMER_LEFT_3_PENALTY=0.180765
PRIMER_RIGHT_3_PENALTY=0.108707
PRIMER_LEFT_3_SEQUENCE=CTTGAAAACAAGTCCACAGTGC
PRIMER_RIGHT_3_SEQUENCE=ACCCAACCTCCCACTAAATCTT
PRIMER_LEFT_3=49,22
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=59.819
PRIMER_RIGHT_3_TM=60.109
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.3153
PRIMER_LEFT_4_PENALTY=0.180765
PRIMER_RIGHT_4_PENALTY=0.134571
PRIMER_LEFT_4_SEQUENCE=TTGAAAACAAGTCCACAGTGCT
PRIMER_RIGHT_4_SEQUENCE=CCAACCTCCCACTAAATCTTTG
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=172,22
PRIMER_LEFT_4_TM=59.819
PRIMER_RIGHT_4_TM=59.865
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_02634 Ps_contig_mira-and-tgicl-ass_franssen_23216
SEQUENCE=GGTCTGCGCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCAGACCTTTGCCGGAGAATGGTCTCGGCAAGCTCCGAAAACACCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGAATCAGGGGGTGGAAACGTGAGATCCTACGGTGGGGATTCGTTAGAGGGTGCAGTGAAAGTGACACGTAGTATCGTGATAGTGAAACCAGCTGGG
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3621
PRIMER_LEFT_PENALTY=0.275745
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT=22,22
PRIMER_RIGHT=199,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.3643
PRIMER_LEFT_1_PENALTY=0.275745
PRIMER_RIGHT_1_PENALTY=0.088563
PRIMER_LEFT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_1_SEQUENCE=CCTGATTCCTTGTCGGAGATAG
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=143,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=60.089
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=0.6994
PRIMER_LEFT_2_PENALTY=0.275745
PRIMER_RIGHT_2_PENALTY=0.423703
PRIMER_LEFT_2_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_2_SEQUENCE=ATTCCTTGTCGGAGATAGATCG
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=139,22
PRIMER_LEFT_2_TM=60.276
PRIMER_RIGHT_2_TM=59.576
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=0.8750
PRIMER_LEFT_3_PENALTY=0.275745
PRIMER_RIGHT_3_PENALTY=0.599276
PRIMER_LEFT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_3_SEQUENCE=GTCACTTTCACTGCACCCTCTA
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=200,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.401
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=179
PRIMER_PAIR_PENALTY_4=0.9862
PRIMER_LEFT_4_PENALTY=0.275745
PRIMER_RIGHT_4_PENALTY=0.710449
PRIMER_LEFT_4_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_4_SEQUENCE=ACTTTCACTGCACCCTCTAACG
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=197,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=60.710
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=176
=
PRIMER_SEQUENCE_ID=SSR_02635 Ps_contig_mira-and-tgicl-ass_franssen_23233
SEQUENCE=CACTACCAAATATCACATTTCAATTCTTCTTGAGTTCAGTACAATGGATTCCAGAAAAGCAATGCTCATTCTTGGCCTATTGGCCATGGTTCTTCTTATTTCATCAGAGGTGTCAGCTAGGGAATTAACTGAGGAGGTTGAAAAGTCAGATGAAGTAAACGATGCTAAATATGGAGGTTATGGACGTGGTGGTGGTTACCATAATGGWGGCGGTTACCACAATGGTGGAGGAGGATACAATGGCGGTGGCGGTTACCACAAYGGTGGAGGTGGTTACCATAATGGTGGAGGTGGTTACAATGGCGGTGGAGGTTACCATAATGGTGGTGGTGGTTACCATGGTGGAGGTGGACACGGTGGACATGGTGGTGCTTCCAACAACGGTAACTGATAATATCAACATTCTCAGTAATAAATATCATGAATTAATAATGTTGGATGTTGAAAAAAAAAGAGAGGTATAGATAGACA
TARGET=322,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02636 Ps_contig_mira-and-tgicl-ass_franssen_23234
SEQUENCE=AACGTAATCAAAGTCAACAGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTTCCATCTAGCTTGTTTTTTGCTTCAGTTTGTGT
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02637 Ps_contig_mira-and-tgicl-ass_franssen_23237
SEQUENCE=TGAATACTTTCTCARGTTTTAGGTCACTTCTATGAAGTTACTGATGCAAACAAGTTTGAAGAACTTGTTCAGCTGGTGTTTTGATACCAAGTTCTGTAATAAGCTTACCAGCTAGCACCATTTTTGTACAAGAAACTAAATGTTTAATGAAAGAAAGAAAAGAAAAGAGATATTATGATCCCCGMGTACT
TARGET=149,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02638 Ps_contig_mira-and-tgicl-ass_franssen_23237
SEQUENCE=TGAATACTTTCTCARGTTTTAGGTCACTTCTATGAAGTTACTGATGCAAACAAGTTTGAAGAACTTGTTCAGCTGGTGTTTTGATACCAAGTTCTGTAATAAGCTTACCAGCTAGCACCATTTTTGTACAAGAAACTAAATGTTTAATGAAAGAAAGAAAAGAAAAGAGATATTATGATCCCCGMGTACT
TARGET=154,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02639 Ps_contig_mira-and-tgicl-ass_franssen_23239
SEQUENCE=GTCGGGGGAAGAGTAAAACGTAGAAACTTTTAGTACCAGAGTTTTGGGAGAGTGATATGGCACAGTGGTCGTTGCTTATCGTTTTGTTCTGCGTCGCCTCCGCCGCCGCCGGATTCAGCTTCCATGACCTCCAATCCTATTCGAATGGTCTCCGACGTGGAGGAGCTAGTTTACTTCGAGGTGAGTCGGAAGAAACGTCGTCACGCCGTCTTCTTCSGCCCCGGTTCGACCTAACAG
TARGET=100,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02640 Ps_contig_mira-and-tgicl-ass_franssen_23255
SEQUENCE=TAGTTGAGGATAANAAGATTGTGGGATGACAAAACTTACAGCCTAATTTGCATCAGAGTCATCAACTAATNTGGGTTTAGGGGCGTAAACATATGGTTTATGATTATTGTAATGATGCTTCTAGGTTTCCTGTTACTCCTCTTGAGTGTCTTCATCATCGTCATAGATTATTCTAATCATGTCAAACTTGTGTATTATTATTATGGCTGGAAATGGTGAATGTTGGTGTAAGAAMTGTTCCACTTGTGTCATATTATTGTATGATATTATTGGCAAACTATATATAATAAGTGGAGAGAAGTGTTAAAAATAAGGTGATGTCAACTTGTGTATTATTATGGGTAGAAATGGT
TARGET=193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02641 Ps_contig_mira-and-tgicl-ass_franssen_23268
SEQUENCE=CCAGTATCTAGCAGTGGTACGGTGGAGGAGGTGATGCGTAGATGTAATGTGGTGGAGGAGGGGAATAGACCGGTGGAGGTGATGAGTAAACAGGTGGAGGAGGAGACTTATATTTGTAAATGGGTGGTGGCGAGTAAACTGGTGGAGGAGGAGATTTATATTTGTAAACTGGTGGTGGTGAATAAATCGGTGGAGGAGAAGATATGTGCTTGTAAGGAGGAGGGGGTGAATTGTACTTGTAGACTGGTGGAGGTGGTGATGAGTACTTGTAAGGAGGCGGTGGTGGAGATTGATATTTGTATGGTGGAGGGGGAGATTTGTAAACCGGTGGTGGTGGTGATGGGTATTTGTATGATTTCTTAGGTGGTGGTGGTGGAGATTGATATTTGTGTTGTGTATTTTGGAAGCCAACAAAGGAAGAAACTTCAA
TARGET=327,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1497
PRIMER_LEFT_PENALTY=0.028056
PRIMER_RIGHT_PENALTY=0.121635
PRIMER_LEFT_SEQUENCE=TGGAGGTGGTGATGAGTACTTG
PRIMER_RIGHT_SEQUENCE=CCTTTGTTGGCTTCCAAAATAC
PRIMER_LEFT=247,22
PRIMER_RIGHT=416,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=59.878
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.2462
PRIMER_LEFT_1_PENALTY=0.150698
PRIMER_RIGHT_1_PENALTY=0.095538
PRIMER_LEFT_1_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_RIGHT_1_SEQUENCE=GGCTTCCAAAATACACAACACA
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=408,22
PRIMER_LEFT_1_TM=59.849
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.2806
PRIMER_LEFT_2_PENALTY=0.185055
PRIMER_RIGHT_2_PENALTY=0.095538
PRIMER_LEFT_2_SEQUENCE=TGAGTACTTGTAAGGAGGCGGT
PRIMER_RIGHT_2_SEQUENCE=GGCTTCCAAAATACACAACACA
PRIMER_LEFT_2=259,22
PRIMER_RIGHT_2=408,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=0.2806
PRIMER_LEFT_3_PENALTY=0.185055
PRIMER_RIGHT_3_PENALTY=0.095538
PRIMER_LEFT_3_SEQUENCE=GAGTACTTGTAAGGAGGCGGTG
PRIMER_RIGHT_3_SEQUENCE=GGCTTCCAAAATACACAACACA
PRIMER_LEFT_3=260,22
PRIMER_RIGHT_3=408,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.2806
PRIMER_LEFT_4_PENALTY=0.185055
PRIMER_RIGHT_4_PENALTY=0.095538
PRIMER_LEFT_4_SEQUENCE=TGAGTACTTGTAAGGAGGCGGT
PRIMER_RIGHT_4_SEQUENCE=TGGCTTCCAAAATACACAACAC
PRIMER_LEFT_4=259,22
PRIMER_RIGHT_4=409,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=59.904
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_02642 Ps_contig_mira-and-tgicl-ass_franssen_23268
SEQUENCE=CCAGTATCTAGCAGTGGTACGGTGGAGGAGGTGATGCGTAGATGTAATGTGGTGGAGGAGGGGAATAGACCGGTGGAGGTGATGAGTAAACAGGTGGAGGAGGAGACTTATATTTGTAAATGGGTGGTGGCGAGTAAACTGGTGGAGGAGGAGATTTATATTTGTAAACTGGTGGTGGTGAATAAATCGGTGGAGGAGAAGATATGTGCTTGTAAGGAGGAGGGGGTGAATTGTACTTGTAGACTGGTGGAGGTGGTGATGAGTACTTGTAAGGAGGCGGTGGTGGAGATTGATATTTGTATGGTGGAGGGGGAGATTTGTAAACCGGTGGTGGTGGTGATGGGTATTTGTATGATTTCTTAGGTGGTGGTGGTGGAGATTGATATTTGTGTTGTGTATTTTGGAAGCCAACAAAGGAAGAAACTTCAA
TARGET=363,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1497
PRIMER_LEFT_PENALTY=0.028056
PRIMER_RIGHT_PENALTY=0.121635
PRIMER_LEFT_SEQUENCE=TGGAGGTGGTGATGAGTACTTG
PRIMER_RIGHT_SEQUENCE=CCTTTGTTGGCTTCCAAAATAC
PRIMER_LEFT=247,22
PRIMER_RIGHT=416,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=59.878
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.2462
PRIMER_LEFT_1_PENALTY=0.150698
PRIMER_RIGHT_1_PENALTY=0.095538
PRIMER_LEFT_1_SEQUENCE=ATATTTGTAAATGGGTGGTGGC
PRIMER_RIGHT_1_SEQUENCE=GGCTTCCAAAATACACAACACA
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=408,22
PRIMER_LEFT_1_TM=59.849
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.2806
PRIMER_LEFT_2_PENALTY=0.185055
PRIMER_RIGHT_2_PENALTY=0.095538
PRIMER_LEFT_2_SEQUENCE=TGAGTACTTGTAAGGAGGCGGT
PRIMER_RIGHT_2_SEQUENCE=GGCTTCCAAAATACACAACACA
PRIMER_LEFT_2=259,22
PRIMER_RIGHT_2=408,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=0.2806
PRIMER_LEFT_3_PENALTY=0.185055
PRIMER_RIGHT_3_PENALTY=0.095538
PRIMER_LEFT_3_SEQUENCE=GAGTACTTGTAAGGAGGCGGTG
PRIMER_RIGHT_3_SEQUENCE=GGCTTCCAAAATACACAACACA
PRIMER_LEFT_3=260,22
PRIMER_RIGHT_3=408,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.2806
PRIMER_LEFT_4_PENALTY=0.185055
PRIMER_RIGHT_4_PENALTY=0.095538
PRIMER_LEFT_4_SEQUENCE=TGAGTACTTGTAAGGAGGCGGT
PRIMER_RIGHT_4_SEQUENCE=TGGCTTCCAAAATACACAACAC
PRIMER_LEFT_4=259,22
PRIMER_RIGHT_4=409,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=59.904
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_02643 Ps_contig_mira-and-tgicl-ass_franssen_23270
SEQUENCE=GAAGAAGAAGAAGAAGAAGAAGAGGAGGGTAAGGTCTTTCTTTGTCGCGTGCTGTTATTTTCTCTGTCGTTTCCGTTTCAGACCGAAACCCTAACTCTGCACCGTAACCATGTCGACCAAGCAAGGTGGAAAAGCTAAGCCTTTGAAGAAACCAAAGTCT
TARGET=1,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02644 Ps_contig_mira-and-tgicl-ass_franssen_23280
SEQUENCE=CTGTTTCTTGTCAATTGGCCATACTTTCCCATAGAGGAAAACACACACACATCATGGGTTTGCAGGAGGATTTTGAGGAGCATGCTGCGAAAGTTAAGACTCTAACAGAGAGTCCATCAAATGAAAACTTGCTTATCCTTTATGGATTGTACAAGCAAGCCACTCTTGGACCTGTTACCACCGCTCGTCCTGGGGTTTCAGCCAGAAAGACAGAGACTAAATGGGATGCAGTGGGAAGGTCTTGGTTAGGAAAGGAAAAACTACCGAAAAGGGTAAGTAGGAAAACGG
TARGET=41,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4175
PRIMER_LEFT_PENALTY=0.299343
PRIMER_RIGHT_PENALTY=0.118203
PRIMER_LEFT_SEQUENCE=ATTGGCCATACTTTCCCATAGA
PRIMER_RIGHT_SEQUENCE=CCCTTTTCGGTAGTTTTTCCTT
PRIMER_LEFT=13,22
PRIMER_RIGHT=272,22
PRIMER_LEFT_TM=59.701
PRIMER_RIGHT_TM=59.882
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.4426
PRIMER_LEFT_1_PENALTY=0.324355
PRIMER_RIGHT_1_PENALTY=0.118203
PRIMER_LEFT_1_SEQUENCE=TTGGCCATACTTTCCCATAGAG
PRIMER_RIGHT_1_SEQUENCE=CCCTTTTCGGTAGTTTTTCCTT
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=60.324
PRIMER_RIGHT_1_TM=59.882
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.6171
PRIMER_LEFT_2_PENALTY=0.299343
PRIMER_RIGHT_2_PENALTY=0.317768
PRIMER_LEFT_2_SEQUENCE=ATTGGCCATACTTTCCCATAGA
PRIMER_RIGHT_2_SEQUENCE=GTAACAGGTCCAAGAGTGGCTT
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=177,22
PRIMER_LEFT_2_TM=59.701
PRIMER_RIGHT_2_TM=59.682
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.6326
PRIMER_LEFT_3_PENALTY=0.299343
PRIMER_RIGHT_3_PENALTY=0.333282
PRIMER_LEFT_3_SEQUENCE=ATTGGCCATACTTTCCCATAGA
PRIMER_RIGHT_3_SEQUENCE=CTTTCCTAACCAAGACCTTCCC
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=59.701
PRIMER_RIGHT_3_TM=60.333
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=0.6326
PRIMER_LEFT_4_PENALTY=0.299343
PRIMER_RIGHT_4_PENALTY=0.333282
PRIMER_LEFT_4_SEQUENCE=ATTGGCCATACTTTCCCATAGA
PRIMER_RIGHT_4_SEQUENCE=CCTTTCCTAACCAAGACCTTCC
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=254,22
PRIMER_LEFT_4_TM=59.701
PRIMER_RIGHT_4_TM=60.333
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_02645 Ps_contig_mira-and-tgicl-ass_franssen_23297
SEQUENCE=GTAGCCCCTCCGATCTCCTCTTCCTTGCTGGTTGGTGGTGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGCGGTGGACACTATGGTGGTGGACACTATGGAGGTGGACACTATGGTGGTGGTCACTACGGTCATGGTGGTGGTCATCATGGTGGACATGGGTGGTGCTTCCGAA
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3694
PRIMER_LEFT_PENALTY=1.103690
PRIMER_RIGHT_PENALTY=0.265715
PRIMER_LEFT_SEQUENCE=GATCTCCTCTTCCTTGCTGGTT
PRIMER_RIGHT_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT=11,22
PRIMER_RIGHT=206,22
PRIMER_LEFT_TM=61.104
PRIMER_RIGHT_TM=59.734
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=1.3694
PRIMER_LEFT_1_PENALTY=1.103690
PRIMER_RIGHT_1_PENALTY=0.265715
PRIMER_LEFT_1_SEQUENCE=GATCTCCTCTTCCTTGCTGGTT
PRIMER_RIGHT_1_SEQUENCE=CTCCATAGTGTCCACCACCATA
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=191,22
PRIMER_LEFT_1_TM=61.104
PRIMER_RIGHT_1_TM=59.734
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=1.3996
PRIMER_LEFT_2_PENALTY=1.103690
PRIMER_RIGHT_2_PENALTY=0.295944
PRIMER_LEFT_2_SEQUENCE=GATCTCCTCTTCCTTGCTGGTT
PRIMER_RIGHT_2_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=200,22
PRIMER_LEFT_2_TM=61.104
PRIMER_RIGHT_2_TM=60.296
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=1.4339
PRIMER_LEFT_3_PENALTY=1.168205
PRIMER_RIGHT_3_PENALTY=0.265715
PRIMER_LEFT_3_SEQUENCE=GATCTCCTCTTCCTTGCTGGT
PRIMER_RIGHT_3_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT_3=11,21
PRIMER_RIGHT_3=206,22
PRIMER_LEFT_3_TM=59.832
PRIMER_RIGHT_3_TM=59.734
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=1.4641
PRIMER_LEFT_4_PENALTY=1.168205
PRIMER_RIGHT_4_PENALTY=0.295944
PRIMER_LEFT_4_SEQUENCE=GATCTCCTCTTCCTTGCTGGT
PRIMER_RIGHT_4_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_4=11,21
PRIMER_RIGHT_4=200,22
PRIMER_LEFT_4_TM=59.832
PRIMER_RIGHT_4_TM=60.296
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_02646 Ps_contig_mira-and-tgicl-ass_franssen_23302
SEQUENCE=GTCAATATCTCCACCAAACAGCTTGTATTTTGTTGTATTCTTTTTGTTCTCATCATCTACTTCTTCAATACTTTCATTACATGTGTGTACCTCCCCATCTATTGTACATAAGTCCAGTGTTTAATGGAATCAGAGTGATGCCAGTCTTCACCATGATGAATCTTGGTATGATGAATTCTTTCACAAAGGTTTTGCACTTCATGAAGTTCTGATGCAAACAGTTTGAAGAACTTGTCAGCTGGTTGTTTTGATACCAAGTTCTAGTAATAAGCTTACCAGCTAGCCACCTTATTTTTAGAACAAAGTAAAACTTAAATTGTTTAAATAGAAAAGAAAAGAAAAAAGG
TARGET=327,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02647 Ps_contig_mira-and-tgicl-ass_franssen_23307
SEQUENCE=GAGGGGGAGGCTATGGTGGCCGTCGTGAAGGKGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTAAAGGAAAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAAGTAAGTTGGGTTACTTGTTCTGTAATGGATTCTTGGTTTGTTTCTWTACTGGTNTTCGTTTGGTTTTCTGGTTGTTTCGTTTTGGTTTGGTT
TARGET=33,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02648 Ps_contig_mira-and-tgicl-ass_franssen_23307
SEQUENCE=GAGGGGGAGGCTATGGTGGCCGTCGTGAAGGKGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTAAAGGAAAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAAGTAAGTTGGGTTACTTGTTCTGTAATGGATTCTTGGTTTGTTTCTWTACTGGTNTTCGTTTGGTTTTCTGGTTGTTTCGTTTTGGTTTGGTT
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02649 Ps_contig_mira-and-tgicl-ass_franssen_23307
SEQUENCE=GAGGGGGAGGCTATGGTGGCCGTCGTGAAGGKGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTAAAGGAAAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAAGTAAGTTGGGTTACTTGTTCTGTAATGGATTCTTGGTTTGTTTCTWTACTGGTNTTCGTTTGGTTTTCTGGTTGTTTCGTTTTGGTTTGGTT
TARGET=128,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02650 Ps_contig_mira-and-tgicl-ass_franssen_23329
SEQUENCE=GTCGGGGTACTAACTAACTATTTTGTCTTAGTTAGTCATCGAAGAGAACAACATCAAAGTGCAAGGAGTCTCAACGCCCTCATGGCCACCTCTGCTATCCAACAATCAAGCTTTCACTGGTAAGACTGGTTTGAGGCAGGAAATNATTCATCGAGGGAAAGCTGGAAACTTTGGCCAANCGTCNCTTTAACCA
TARGET=9,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02651 Ps_contig_mira-and-tgicl-ass_franssen_23354
SEQUENCE=GAACGTTTCTCKGTCTACATTTTGTTGTTCCTCTCTCTCTAACACCGAGTGATGGGCACTTTCCAGCTGTGGTGGGAGGCCCAAAAGGAGCYCATCTTCTCCTTCACCGGTCTATCACCGACCACTTTCTTCACTGGATTGGCGATTCTATTTGCTCTTWTCTACGTCGTTTCCGGTCTCTTGYKSCTCCTCCTCCTCCGACCGTCACCACCAAACCCGCCACATCCAAGCAGACGGTTCCCCCTCTCCCGACCCCCCGTTNAGCTAGGTGAAATCCAACCCGGGAAAGAAAGGACGGCCTACCGAAAACGGGTCCCCTTAACGGAATTCGGGGACCAAAACCGGTAACTTACCCGAAAACCCGGGTAAAACGTTTAAAACCGGGTTCCCCCCCGG
TARGET=31,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02652 Ps_contig_mira-and-tgicl-ass_franssen_23354
SEQUENCE=GAACGTTTCTCKGTCTACATTTTGTTGTTCCTCTCTCTCTAACACCGAGTGATGGGCACTTTCCAGCTGTGGTGGGAGGCCCAAAAGGAGCYCATCTTCTCCTTCACCGGTCTATCACCGACCACTTTCTTCACTGGATTGGCGATTCTATTTGCTCTTWTCTACGTCGTTTCCGGTCTCTTGYKSCTCCTCCTCCTCCGACCGTCACCACCAAACCCGCCACATCCAAGCAGACGGTTCCCCCTCTCCCGACCCCCCGTTNAGCTAGGTGAAATCCAACCCGGGAAAGAAAGGACGGCCTACCGAAAACGGGTCCCCTTAACGGAATTCGGGGACCAAAACCGGTAACTTACCCGAAAACCCGGGTAAAACGTTTAAAACCGGGTTCCCCCCCGG
TARGET=187,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02653 Ps_contig_mira-and-tgicl-ass_franssen_23363
SEQUENCE=GCCTATCCCCTGTTGCGTGTCTCAGACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAAGCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAACATTTACTTATTCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGAAGGCATAAWGTGAACGATTTTGTTCCTAATAAAGACAANACTCAATAGAAATTGGCCGGCGTTTAATTTAAAAGGATCATACAAGAAAGACTGACTAACTATTAC
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02654 Ps_contig_mira-and-tgicl-ass_franssen_23363
SEQUENCE=GCCTATCCCCTGTTGCGTGTCTCAGACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAAGCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAACATTTACTTATTCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGAAGGCATAAWGTGAACGATTTTGTTCCTAATAAAGACAANACTCAATAGAAATTGGCCGGCGTTTAATTTAAAAGGATCATACAAGAAAGACTGACTAACTATTAC
TARGET=114,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02655 Ps_contig_mira-and-tgicl-ass_franssen_23364
SEQUENCE=GTCGGGGTACCTATTTGTATWAAGAATACAATTATCAAAAGCATAACAATCTTTTCAATTTCATTGCAATATACACACACGATGGCCGCATCATCATCATCATCCATGACTCTCTCTTCTCCAACCTTGGCTGGCAAGCAACTCAAGCTGACCCCATCAAGCCAAGAATTGGGAGCTGCAAGGTTCACCATGAGGAAGTCTGCTACCAA
TARGET=89,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02656 Ps_contig_mira-and-tgicl-ass_franssen_23375
SEQUENCE=GGGGCAAGTTCAGTTGCAATTCGCGACTAAATATGGGAAGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATNTGGCGGTGGACACTATGGGTGGTGGACACTATGGAGGTGGA
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6505
PRIMER_LEFT_PENALTY=0.028588
PRIMER_RIGHT_PENALTY=0.621889
PRIMER_LEFT_SEQUENCE=AATATGGGAAGGAGGTTACGGT
PRIMER_RIGHT_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT=29,22
PRIMER_RIGHT=174,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=59.378
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=0.7718
PRIMER_LEFT_1_PENALTY=0.028588
PRIMER_RIGHT_1_PENALTY=0.743211
PRIMER_LEFT_1_SEQUENCE=AATATGGGAAGGAGGTTACGGT
PRIMER_RIGHT_1_SEQUENCE=TAGTGTCCACCACCCATAGTGT
PRIMER_LEFT_1=29,22
PRIMER_RIGHT_1=236,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.257
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.3000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=1.0388
PRIMER_LEFT_2_PENALTY=0.416884
PRIMER_RIGHT_2_PENALTY=0.621889
PRIMER_LEFT_2_SEQUENCE=AAATATGGGAAGGAGGTTACGG
PRIMER_RIGHT_2_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=174,22
PRIMER_LEFT_2_TM=60.417
PRIMER_RIGHT_2_TM=59.378
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=1.0570
PRIMER_LEFT_3_PENALTY=0.435099
PRIMER_RIGHT_3_PENALTY=0.621889
PRIMER_LEFT_3_SEQUENCE=ATATGGGAAGGAGGTTACGGTC
PRIMER_RIGHT_3_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=60.435
PRIMER_RIGHT_3_TM=59.378
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=1.1181
PRIMER_LEFT_4_PENALTY=0.496243
PRIMER_RIGHT_4_PENALTY=0.621889
PRIMER_LEFT_4_SEQUENCE=ATTCGCGACTAAATATGGGAAG
PRIMER_RIGHT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=174,22
PRIMER_LEFT_4_TM=59.504
PRIMER_RIGHT_4_TM=59.378
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_02657 Ps_contig_mira-and-tgicl-ass_franssen_23375
SEQUENCE=GGGGCAAGTTCAGTTGCAATTCGCGACTAAATATGGGAAGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATNTGGCGGTGGACACTATGGGTGGTGGACACTATGGAGGTGGA
TARGET=103,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6505
PRIMER_LEFT_PENALTY=0.028588
PRIMER_RIGHT_PENALTY=0.621889
PRIMER_LEFT_SEQUENCE=AATATGGGAAGGAGGTTACGGT
PRIMER_RIGHT_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT=29,22
PRIMER_RIGHT=174,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=59.378
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=0.7718
PRIMER_LEFT_1_PENALTY=0.028588
PRIMER_RIGHT_1_PENALTY=0.743211
PRIMER_LEFT_1_SEQUENCE=AATATGGGAAGGAGGTTACGGT
PRIMER_RIGHT_1_SEQUENCE=TAGTGTCCACCACCCATAGTGT
PRIMER_LEFT_1=29,22
PRIMER_RIGHT_1=236,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.257
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.3000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=1.0388
PRIMER_LEFT_2_PENALTY=0.416884
PRIMER_RIGHT_2_PENALTY=0.621889
PRIMER_LEFT_2_SEQUENCE=AAATATGGGAAGGAGGTTACGG
PRIMER_RIGHT_2_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=174,22
PRIMER_LEFT_2_TM=60.417
PRIMER_RIGHT_2_TM=59.378
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=1.0570
PRIMER_LEFT_3_PENALTY=0.435099
PRIMER_RIGHT_3_PENALTY=0.621889
PRIMER_LEFT_3_SEQUENCE=ATATGGGAAGGAGGTTACGGTC
PRIMER_RIGHT_3_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=60.435
PRIMER_RIGHT_3_TM=59.378
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=1.1181
PRIMER_LEFT_4_PENALTY=0.496243
PRIMER_RIGHT_4_PENALTY=0.621889
PRIMER_LEFT_4_SEQUENCE=ATTCGCGACTAAATATGGGAAG
PRIMER_RIGHT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=174,22
PRIMER_LEFT_4_TM=59.504
PRIMER_RIGHT_4_TM=59.378
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_02658 Ps_contig_mira-and-tgicl-ass_franssen_23380
SEQUENCE=GAAGAGTAAAACGTAGAAACTTTTAGTACCAGAGTTTTGGGAGAGTGATATGGCACAGTGGTCGTTGCTTATCGTTTTGTTCTGCGTCGCCTCCGCCGCCGCCGGATTCAGCTTCCATGACTCCAATCCTATTCGAATGGTCTCCGACGTGGAGGAGCAGTTACTTCAGGTGATCGGAGAAACAGTCTACCTGATCAAGTTGTTGTTCAGAGGATAGAAGAAAAACTATCTG
TARGET=93,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3637
PRIMER_LEFT_PENALTY=0.061818
PRIMER_RIGHT_PENALTY=0.301926
PRIMER_LEFT_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_SEQUENCE=CCTGAAGTAACTGCTCCTCCAC
PRIMER_LEFT=56,22
PRIMER_RIGHT=169,22
PRIMER_LEFT_TM=60.062
PRIMER_RIGHT_TM=60.302
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=0.3637
PRIMER_LEFT_1_PENALTY=0.061818
PRIMER_RIGHT_1_PENALTY=0.301926
PRIMER_LEFT_1_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_1_SEQUENCE=CACCTGAAGTAACTGCTCCTCC
PRIMER_LEFT_1=56,22
PRIMER_RIGHT_1=171,22
PRIMER_LEFT_1_TM=60.062
PRIMER_RIGHT_1_TM=60.302
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=116
PRIMER_PAIR_PENALTY_2=0.3717
PRIMER_LEFT_2_PENALTY=0.061818
PRIMER_RIGHT_2_PENALTY=0.309858
PRIMER_LEFT_2_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_2_SEQUENCE=GAAGTAACTGCTCCTCCACGTC
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=166,22
PRIMER_LEFT_2_TM=60.062
PRIMER_RIGHT_2_TM=60.310
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=0.4859
PRIMER_LEFT_3_PENALTY=0.184005
PRIMER_RIGHT_3_PENALTY=0.301926
PRIMER_LEFT_3_SEQUENCE=CGTTGCTTATCGTTTTGTTCTG
PRIMER_RIGHT_3_SEQUENCE=CCTGAAGTAACTGCTCCTCCAC
PRIMER_LEFT_3=62,22
PRIMER_RIGHT_3=169,22
PRIMER_LEFT_3_TM=59.816
PRIMER_RIGHT_3_TM=60.302
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=108
PRIMER_PAIR_PENALTY_4=0.4859
PRIMER_LEFT_4_PENALTY=0.184005
PRIMER_RIGHT_4_PENALTY=0.301926
PRIMER_LEFT_4_SEQUENCE=CGTTGCTTATCGTTTTGTTCTG
PRIMER_RIGHT_4_SEQUENCE=CACCTGAAGTAACTGCTCCTCC
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=171,22
PRIMER_LEFT_4_TM=59.816
PRIMER_RIGHT_4_TM=60.302
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_02659 Ps_contig_mira-and-tgicl-ass_franssen_23382
SEQUENCE=CAGAATGGCAGTGACCAGCGGTTACTTGTATGCCAGAAACCATTTTTGAAAAAATGATTGGACACTACGGTCATGGTGGTGGTGGTTACCATGGTGGTGGTGGCGGTTACCATGGTGGACATGGAGGACATGGAGGACATGGTGGACACGGTGGTGCTTCAGACAATGGAAACTGATTATATCATCATACATGATGCACATTACAAAAGTTAATTAAGTTTCATAGAATGTAATTAGGTTTAGGTAARKTAAAAAAGTAAAAGGAGGTAACAAATGTCATTCTATTTAGTTGGTTKGTTGGTTTATTATTGTTATTTA
TARGET=74,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02660 Ps_contig_mira-and-tgicl-ass_franssen_23382
SEQUENCE=CAGAATGGCAGTGACCAGCGGTTACTTGTATGCCAGAAACCATTTTTGAAAAAATGATTGGACACTACGGTCATGGTGGTGGTGGTTACCATGGTGGTGGTGGCGGTTACCATGGTGGACATGGAGGACATGGAGGACATGGTGGACACGGTGGTGCTTCAGACAATGGAAACTGATTATATCATCATACATGATGCACATTACAAAAGTTAATTAAGTTTCATAGAATGTAATTAGGTTTAGGTAARKTAAAAAAGTAAAAGGAGGTAACAAATGTCATTCTATTTAGTTGGTTKGTTGGTTTATTATTGTTATTTA
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02661 Ps_contig_mira-and-tgicl-ass_franssen_23385
SEQUENCE=CGGGGAACTCTCCAGTCAAGTAGGATGGAGTACTTCTACCGGTAGTAATTGGGTCCTTAAGTTACTTAACACGGTCTGGTCCGTACCATGGGCTCCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGCTTGATGGGKTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGTGTGTGTATATTGCAATGAAATTGAAAAGATTGTTATGTCTTTTGATAATTGTATTT
TARGET=222,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02662 Ps_contig_mira-and-tgicl-ass_franssen_23391
SEQUENCE=AAGTAAAAAAAGACTCACAACACATAAGAGATACTTGTAATCTACAACCAATTATTACTTTTCTTTCTTTCAATTAGTCTGTCTCAACACCTAATCCATACAACACACCAGAGTTATTTCTCTTGTTTCGCCTATTGAAGAACGTACTCCCTCTAATTTTACTAAATGCAACAAGCAGTGAAGTAAACT
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02663 Ps_contig_mira-and-tgicl-ass_franssen_23437
SEQUENCE=CAATATCTACAAAATGATTTGTAGTTTTTTATTAGTAGGAATCAAATTCTGTAGTTCGCAATTTACAACACACACACACTGCCGCACCAACAACAAAACAATTGAGCTGATTCACATGAGAGAAGAGACCATGTTCTAACATACGAAAACCCTAATTCTCTTTATTCCCGATTGAAANAAAATAAACCCACCAACCAAAACAAAGTATAGTGGTCTTTTTGAGAAGAAGAAATAAACTCACACACNT
TARGET=68,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1282
PRIMER_LEFT_PENALTY=2.113497
PRIMER_RIGHT_PENALTY=0.014720
PRIMER_LEFT_SEQUENCE=GGAATCAAATTCTGTAGTTCGC
PRIMER_RIGHT_SEQUENCE=ATACTTTGTTTTGGTTGGTGGG
PRIMER_LEFT=37,22
PRIMER_RIGHT=207,22
PRIMER_LEFT_TM=57.887
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=2.6385
PRIMER_LEFT_1_PENALTY=2.113497
PRIMER_RIGHT_1_PENALTY=0.524968
PRIMER_LEFT_1_SEQUENCE=GGAATCAAATTCTGTAGTTCGC
PRIMER_RIGHT_1_SEQUENCE=TACTTTGTTTTGGTTGGTGGGT
PRIMER_LEFT_1=37,22
PRIMER_RIGHT_1=206,22
PRIMER_LEFT_1_TM=57.887
PRIMER_RIGHT_1_TM=60.525
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=3.3646
PRIMER_LEFT_2_PENALTY=2.113497
PRIMER_RIGHT_2_PENALTY=1.251135
PRIMER_LEFT_2_SEQUENCE=GGAATCAAATTCTGTAGTTCGC
PRIMER_RIGHT_2_SEQUENCE=TACTTTGTTTTGGTTGGTGGG
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=206,21
PRIMER_LEFT_2_TM=57.887
PRIMER_RIGHT_2_TM=59.749
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=3.4000
PRIMER_LEFT_3_PENALTY=3.385311
PRIMER_RIGHT_3_PENALTY=0.014720
PRIMER_LEFT_3_SEQUENCE=GTAGGAATCAAATTCTGTAGTTCGC
PRIMER_RIGHT_3_SEQUENCE=ATACTTTGTTTTGGTTGGTGGG
PRIMER_LEFT_3=34,25
PRIMER_RIGHT_3=207,22
PRIMER_LEFT_3_TM=59.615
PRIMER_RIGHT_3_TM=60.015
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=3.4658
PRIMER_LEFT_4_PENALTY=2.113497
PRIMER_RIGHT_4_PENALTY=1.352339
PRIMER_LEFT_4_SEQUENCE=GGAATCAAATTCTGTAGTTCGC
PRIMER_RIGHT_4_SEQUENCE=TCGGGAATAAAGAGAATTAGGG
PRIMER_LEFT_4=37,22
PRIMER_RIGHT_4=170,22
PRIMER_LEFT_4_TM=57.887
PRIMER_RIGHT_4_TM=58.648
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_02664 Ps_contig_mira-and-tgicl-ass_franssen_23456
SEQUENCE=GAACCTGACTCTCAAGTGGAAAGCATCCTTTCCAGCAAACTTAGTCATGTACTTGTTGCAAGCAATACGGGCAGCTTCAAGTGCCTCACTTGAAACATTCTCCTTCTCCCAACTGACAAGATGGACACAGAGAGGGAACTCGTCAACACCCTTCTTCTTCATGCCAACATCGTAGATCCTGATCTTGGGATCAGGAACACCACGACAGAATCGTGACTTTGGATATGGTCTTTGTTTTAATTGACGTAACTCTTGCTAGGTTCCTTCCTTCCCCTAGTTCGGCCGGACGGCGT
TARGET=261,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02665 Ps_contig_mira-and-tgicl-ass_franssen_23479
SEQUENCE=TCGGRKTCCATTCCATTCCATTCACTCATCAAACCAAAAACAATGGCAGCATCAGCATCATCCATGATCTCAACAAACGCCCTAACATCCACACTCCAAAAAACACCAAACACACAACCTCAAACCATCAAACCTATGTTTCCAAGGCTTCAAACCCTTAACAGATCAACAAACTGACACAGAACAAAAAGAGATTCTCATCCYTYGTTGTACAAAGACTGAACCTACCTAA
TARGET=7,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02666 Ps_contig_mira-and-tgicl-ass_franssen_23498
SEQUENCE=TCCTCTGCTGCTGGAGCTTCCACTGWTTCAAACTGTTTTGTTCTCAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTTGCTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTTCACTTCTTCAACCGATTCCTCTAGTTTTCTTTAGGTGCTTTCTAAGTTCTCTTCCTTTGCTTCCTCTGTTACCACTTCCTTGTTCTCAACTTCAAGTGGATTCGTTGGGGTTCGGGANCTATCTA
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02667 Ps_contig_mira-and-tgicl-ass_franssen_23538
SEQUENCE=TTGCAAACTTAGACCTCAATCCATTATTATTATTGGAACAGTTGCTCCTCAATTGCAGTTCTTTCTGCTACACGGTTTTCATTGTCGCTGTCACACTCATCACTGCTACTGCCACTGTCACTGCTGTGACCTGATATGCCATCCTTGATCCTCTGGAACATGTTTCTCCTCTCCCTCCTGTTTCTCCCTTGGCATTTTGCTGCAGTGGTGCTAGTAACAATGGTGGGGCCAGTAGCGGCGTGATTGTAGCGTGTTTGGTTGCATGTGTTACTTTGATATTTGGAAGCATGTCCAGGTTGATAAATCACCTCGGTTTGGCTATAGCATTGGGAATTGGTTCCATCATTGTGGTGTCCTTTGTAGAAGTCAGTTACCTTATGTCCGATTGAGTTATGTTGCTGCTGCTGCTGCTGGTGGTGTGTNTTGTNTCAT
TARGET=23,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2936
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=0.040146
PRIMER_LEFT_SEQUENCE=TGCAAACTTAGACCTCAATCCA
PRIMER_RIGHT_SEQUENCE=CTGGACATGCTTCCAAATATCA
PRIMER_LEFT=1,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=59.960
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=0.3628
PRIMER_LEFT_1_PENALTY=0.253482
PRIMER_RIGHT_1_PENALTY=0.109273
PRIMER_LEFT_1_SEQUENCE=TGCAAACTTAGACCTCAATCCA
PRIMER_RIGHT_1_SEQUENCE=AAACAGGAGGGAGAGGAGAAAC
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=182,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=0.3636
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.110131
PRIMER_LEFT_2_SEQUENCE=TGCAAACTTAGACCTCAATCCA
PRIMER_RIGHT_2_SEQUENCE=GAGGAGAAACATGTTCCAGAGG
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=170,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.3636
PRIMER_LEFT_3_PENALTY=0.253482
PRIMER_RIGHT_3_PENALTY=0.110131
PRIMER_LEFT_3_SEQUENCE=TGCAAACTTAGACCTCAATCCA
PRIMER_RIGHT_3_SEQUENCE=AGGAGAAACATGTTCCAGAGGA
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=169,22
PRIMER_LEFT_3_TM=59.747
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=0.5604
PRIMER_LEFT_4_PENALTY=0.253482
PRIMER_RIGHT_4_PENALTY=0.306903
PRIMER_LEFT_4_SEQUENCE=TGCAAACTTAGACCTCAATCCA
PRIMER_RIGHT_4_SEQUENCE=AGGATCAAGGATGGCATATCAG
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=151,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=60.307
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_02668 Ps_contig_mira-and-tgicl-ass_franssen_23538
SEQUENCE=TTGCAAACTTAGACCTCAATCCATTATTATTATTGGAACAGTTGCTCCTCAATTGCAGTTCTTTCTGCTACACGGTTTTCATTGTCGCTGTCACACTCATCACTGCTACTGCCACTGTCACTGCTGTGACCTGATATGCCATCCTTGATCCTCTGGAACATGTTTCTCCTCTCCCTCCTGTTTCTCCCTTGGCATTTTGCTGCAGTGGTGCTAGTAACAATGGTGGGGCCAGTAGCGGCGTGATTGTAGCGTGTTTGGTTGCATGTGTTACTTTGATATTTGGAAGCATGTCCAGGTTGATAAATCACCTCGGTTTGGCTATAGCATTGGGAATTGGTTCCATCATTGTGGTGTCCTTTGTAGAAGTCAGTTACCTTATGTCCGATTGAGTTATGTTGCTGCTGCTGCTGCTGGTGGTGTGTNTTGTNTCAT
TARGET=397,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02669 Ps_contig_mira-and-tgicl-ass_franssen_23559
SEQUENCE=GATTCTAACTGTTTTGTTNTTCAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTTGCTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTTCACTTCTTCAACCGATTCCTCAGTTTCTTTAGGTGCTTCTAAGTTCTACTTCCTTTGCTTCCTCTGTTACCACTTCCTTGTTCTCAACTTCAAGTGGATCTTGGGGTTTCGGGAGCATCTACAGCTTCTCCTGCTGCAGTTTGTTTCTTGGTTTGGGTTTTTTCGGTTGTTT
TARGET=44,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2620
PRIMER_LEFT_PENALTY=0.259704
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=TTCAATCACATCCTCTGCAGTT
PRIMER_RIGHT_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_LEFT=19,22
PRIMER_RIGHT=125,22
PRIMER_LEFT_TM=59.740
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=0.4243
PRIMER_LEFT_1_PENALTY=0.259704
PRIMER_RIGHT_1_PENALTY=0.164560
PRIMER_LEFT_1_SEQUENCE=TTCAATCACATCCTCTGCAGTT
PRIMER_RIGHT_1_SEQUENCE=GTGGTAACAGAGGAAGCAAAGG
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=187,22
PRIMER_LEFT_1_TM=59.740
PRIMER_RIGHT_1_TM=60.165
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.4974
PRIMER_LEFT_2_PENALTY=0.259704
PRIMER_RIGHT_2_PENALTY=0.237699
PRIMER_LEFT_2_SEQUENCE=TTCAATCACATCCTCTGCAGTT
PRIMER_RIGHT_2_SEQUENCE=GGTAACAGAGGAAGCAAAGGAA
PRIMER_LEFT_2=19,22
PRIMER_RIGHT_2=185,22
PRIMER_LEFT_2_TM=59.740
PRIMER_RIGHT_2_TM=59.762
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.5085
PRIMER_LEFT_3_PENALTY=0.259704
PRIMER_RIGHT_3_PENALTY=0.248820
PRIMER_LEFT_3_SEQUENCE=TTCAATCACATCCTCTGCAGTT
PRIMER_RIGHT_3_SEQUENCE=CTGAGGAATCGGTTGAAGAAGT
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=137,22
PRIMER_LEFT_3_TM=59.740
PRIMER_RIGHT_3_TM=59.751
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=0.9204
PRIMER_LEFT_4_PENALTY=0.259704
PRIMER_RIGHT_4_PENALTY=0.660713
PRIMER_LEFT_4_SEQUENCE=TTCAATCACATCCTCTGCAGTT
PRIMER_RIGHT_4_SEQUENCE=GGAAGTGGTAACAGAGGAAGCA
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=191,22
PRIMER_LEFT_4_TM=59.740
PRIMER_RIGHT_4_TM=60.661
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_02670 Ps_contig_mira-and-tgicl-ass_franssen_23572
SEQUENCE=GTGGTATCAACGCAGAGTTGTTGGTAAGTTKKTAAAGGTATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTTCTTTTTTTACTGTGTACTACTTCTAACCAAGGTAAAG
TARGET=180,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02671 Ps_contig_mira-and-tgicl-ass_franssen_23576
SEQUENCE=CTACACATGTTGGCGATAATCAAGTTAGTCACTTATCATTGTCTATTTCTTTCTTTCTACTTTTCCTAGGGGCAAAGTTTGTGGCATATGCCCATGCATTGTTGTTCACTGGATCAGCCAGGTGGTCAGCAAGATTTTCTATTGGTCCTTTTCCTGTTACAATAGCTTGAACGAAGAATCCAAACATTGAGAACATCGCTAACCTACCATTCTTCAGTTCTTTCACCTTCA
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2418
PRIMER_LEFT_PENALTY=0.110942
PRIMER_RIGHT_PENALTY=0.130883
PRIMER_LEFT_SEQUENCE=ACACATGTTGGCGATAATCAAG
PRIMER_RIGHT_SEQUENCE=TAGCGATGTTCTCAATGTTTGG
PRIMER_LEFT=2,22
PRIMER_RIGHT=200,22
PRIMER_LEFT_TM=59.889
PRIMER_RIGHT_TM=60.131
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.3423
PRIMER_LEFT_1_PENALTY=0.110942
PRIMER_RIGHT_1_PENALTY=0.231323
PRIMER_LEFT_1_SEQUENCE=ACACATGTTGGCGATAATCAAG
PRIMER_RIGHT_1_SEQUENCE=TGGTAGGTTAGCGATGTTCTCA
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=208,22
PRIMER_LEFT_1_TM=59.889
PRIMER_RIGHT_1_TM=59.769
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.6703
PRIMER_LEFT_2_PENALTY=0.110942
PRIMER_RIGHT_2_PENALTY=0.559375
PRIMER_LEFT_2_SEQUENCE=ACACATGTTGGCGATAATCAAG
PRIMER_RIGHT_2_SEQUENCE=CTGATCCAGTGAACAACAATGC
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=116,22
PRIMER_LEFT_2_TM=59.889
PRIMER_RIGHT_2_TM=60.559
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=115
PRIMER_PAIR_PENALTY_3=0.6703
PRIMER_LEFT_3_PENALTY=0.110942
PRIMER_RIGHT_3_PENALTY=0.559375
PRIMER_LEFT_3_SEQUENCE=ACACATGTTGGCGATAATCAAG
PRIMER_RIGHT_3_SEQUENCE=GCTGATCCAGTGAACAACAATG
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=117,22
PRIMER_LEFT_3_TM=59.889
PRIMER_RIGHT_3_TM=60.559
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=116
PRIMER_PAIR_PENALTY_4=0.7717
PRIMER_LEFT_4_PENALTY=0.110942
PRIMER_RIGHT_4_PENALTY=0.660713
PRIMER_LEFT_4_SEQUENCE=ACACATGTTGGCGATAATCAAG
PRIMER_RIGHT_4_SEQUENCE=AGAAAATCTTGCTGACCACCTG
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=139,22
PRIMER_LEFT_4_TM=59.889
PRIMER_RIGHT_4_TM=60.661
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_02672 Ps_contig_mira-and-tgicl-ass_franssen_23655
SEQUENCE=GAACGACATTATAAGTGATCGCATGGTTGAAAATGAGARACTGGAAGGTCAAGAGGTTTTGGATTCGTGACCTTTGCCACGGAGCAATCCATGAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCYCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGYGGTGGCGGTGGATATGGGAGGTGGASGYKRTGGTGGSCGTCGTGAAGGTGGAGGAGGAGGATACGGGTAGTGGAGGAGGG
TARGET=242,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02673 Ps_contig_mira-and-tgicl-ass_franssen_23658
SEQUENCE=TGTTTTGGTAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTNTAAGTTTGGGTTGTAAGATTACAGTATCTCTTATGGTGTTGTGWRTTTCTTTTTTTACNGGTGTACACTT
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02674 Ps_contig_mira-and-tgicl-ass_franssen_23671
SEQUENCE=TGTTTGGTAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATTAGTTTACTCACTGCTTTGTGCTATTTAAGAAATTGGAGGGTATTACCGTTTCTTACAAGTGGACGAAACAGAAGAAACTCTCGTGGTGTTGTTGTATGGTAGTTGGTGTGANACANATATAGAAAGAAAGAAATA
TARGET=171,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02675 Ps_contig_mira-and-tgicl-ass_franssen_23699
SEQUENCE=GTCTTCTACAAACGACAAAGACTTGGGAGTGGTACCACGTGGGAGTTTATTTGGGTTTTGGGTACCGATTATTGGTTGCTGCAGTGGCTGCAATCTACCACCRATACATTCTAAGAGGATCAGCTAATTTAAAGTCTTYYTTGGTATTCCTTCAGGAGCAATGCTTAATGAATGCAACATGTTTGGCCTCAATTATTGTTATGAGTTTGTTGATGTTGTTGTTGTTGTTGTCTACTGGTCCCTGTTGAAGTTTTTGTCCT
TARGET=214,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02676 Ps_contig_mira-and-tgicl-ass_franssen_23717
SEQUENCE=AAGCACCGTCGGAAACCAAAGTTGAAGAGGAAGGTGAAAAGAAACACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTCAAGTGAAGAGGAAGGAGAAGATGGTGAAGAAAAGAAAGAAGAAGAAGGAAAGAGAAGAAAGAAAGAACACTACGGTGCNGTGAGAAGTGAGNTGTGAAGGAACAACGACTAGAGAAGAGT
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2757
PRIMER_LEFT_PENALTY=0.117452
PRIMER_RIGHT_PENALTY=0.158297
PRIMER_LEFT_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_RIGHT_SEQUENCE=ACNGCACCGTAGTGTTCTTTCT
PRIMER_LEFT=58,22
PRIMER_RIGHT=177,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=60.158
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.50
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=0.3023
PRIMER_LEFT_1_PENALTY=0.143968
PRIMER_RIGHT_1_PENALTY=0.158297
PRIMER_LEFT_1_SEQUENCE=GTCGGAAACCAAAGTTGAAGAG
PRIMER_RIGHT_1_SEQUENCE=ACNGCACCGTAGTGTTCTTTCT
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=177,22
PRIMER_LEFT_1_TM=60.144
PRIMER_RIGHT_1_TM=60.158
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.50
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=171
PRIMER_PAIR_PENALTY_2=0.4071
PRIMER_LEFT_2_PENALTY=0.248820
PRIMER_RIGHT_2_PENALTY=0.158297
PRIMER_LEFT_2_SEQUENCE=CTTGGAGAAACTTCACCGATCT
PRIMER_RIGHT_2_SEQUENCE=ACNGCACCGTAGTGTTCTTTCT
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=177,22
PRIMER_LEFT_2_TM=59.751
PRIMER_RIGHT_2_TM=60.158
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.50
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.4994
PRIMER_LEFT_3_PENALTY=0.117452
PRIMER_RIGHT_3_PENALTY=0.381964
PRIMER_LEFT_3_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_RIGHT_3_SEQUENCE=CGTTGTTCCTTCACANCTCACT
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=203,22
PRIMER_LEFT_3_TM=60.117
PRIMER_RIGHT_3_TM=60.382
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.75
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.5035
PRIMER_LEFT_4_PENALTY=0.345221
PRIMER_RIGHT_4_PENALTY=0.158297
PRIMER_LEFT_4_SEQUENCE=ACCAAAGTTGAAGAGGAAGGTG
PRIMER_RIGHT_4_SEQUENCE=ACNGCACCGTAGTGTTCTTTCT
PRIMER_LEFT_4=14,22
PRIMER_RIGHT_4=177,22
PRIMER_LEFT_4_TM=59.655
PRIMER_RIGHT_4_TM=60.158
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.50
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=164
=
PRIMER_SEQUENCE_ID=SSR_02677 Ps_contig_mira-and-tgicl-ass_franssen_23717
SEQUENCE=AAGCACCGTCGGAAACCAAAGTTGAAGAGGAAGGTGAAAAGAAACACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTCAAGTGAAGAGGAAGGAGAAGATGGTGAAGAAAAGAAAGAAGAAGAAGGAAAGAGAAGAAAGAAAGAACACTACGGTGCNGTGAGAAGTGAGNTGTGAAGGAACAACGACTAGAGAAGAGT
TARGET=152,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4994
PRIMER_LEFT_PENALTY=0.117452
PRIMER_RIGHT_PENALTY=0.381964
PRIMER_LEFT_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_RIGHT_SEQUENCE=CGTTGTTCCTTCACANCTCACT
PRIMER_LEFT=58,22
PRIMER_RIGHT=203,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=60.382
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.75
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=0.5259
PRIMER_LEFT_1_PENALTY=0.143968
PRIMER_RIGHT_1_PENALTY=0.381964
PRIMER_LEFT_1_SEQUENCE=GTCGGAAACCAAAGTTGAAGAG
PRIMER_RIGHT_1_SEQUENCE=CGTTGTTCCTTCACANCTCACT
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=203,22
PRIMER_LEFT_1_TM=60.144
PRIMER_RIGHT_1_TM=60.382
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.75
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=0.6308
PRIMER_LEFT_2_PENALTY=0.248820
PRIMER_RIGHT_2_PENALTY=0.381964
PRIMER_LEFT_2_SEQUENCE=CTTGGAGAAACTTCACCGATCT
PRIMER_RIGHT_2_SEQUENCE=CGTTGTTCCTTCACANCTCACT
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=203,22
PRIMER_LEFT_2_TM=59.751
PRIMER_RIGHT_2_TM=60.382
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.75
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.6740
PRIMER_LEFT_3_PENALTY=0.117452
PRIMER_RIGHT_3_PENALTY=0.556510
PRIMER_LEFT_3_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_RIGHT_3_SEQUENCE=AGTCGTTGTTCCTTCACANCTC
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=206,22
PRIMER_LEFT_3_TM=60.117
PRIMER_RIGHT_3_TM=59.443
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.75
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0500
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.7005
PRIMER_LEFT_4_PENALTY=0.143968
PRIMER_RIGHT_4_PENALTY=0.556510
PRIMER_LEFT_4_SEQUENCE=GTCGGAAACCAAAGTTGAAGAG
PRIMER_RIGHT_4_SEQUENCE=AGTCGTTGTTCCTTCACANCTC
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=206,22
PRIMER_LEFT_4_TM=60.144
PRIMER_RIGHT_4_TM=59.443
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.75
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0500
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_02678 Ps_contig_mira-and-tgicl-ass_franssen_23739
SEQUENCE=TGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTTGGCTTGATGGGTTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGGTGCGGCCATCGTGTATTATATTGCAATGAAATTAGAAAAGATTTGTTTATGTCTTTTTAGAATAATTT
TARGET=171,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2512
PRIMER_LEFT_PENALTY=0.301926
PRIMER_RIGHT_PENALTY=0.949261
PRIMER_LEFT_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_SEQUENCE=ATATAATACACGATGGCCGCAC
PRIMER_LEFT=59,22
PRIMER_RIGHT=204,22
PRIMER_LEFT_TM=60.302
PRIMER_RIGHT_TM=60.949
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=1.2512
PRIMER_LEFT_1_PENALTY=0.301926
PRIMER_RIGHT_1_PENALTY=0.949261
PRIMER_LEFT_1_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_1_SEQUENCE=ATATAATACACGATGGCCGCAC
PRIMER_LEFT_1=60,22
PRIMER_RIGHT_1=204,22
PRIMER_LEFT_1_TM=60.302
PRIMER_RIGHT_1_TM=60.949
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=1.5081
PRIMER_LEFT_2_PENALTY=0.301926
PRIMER_RIGHT_2_PENALTY=1.206212
PRIMER_LEFT_2_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_2_SEQUENCE=ATATAATACACGATGGCCGCA
PRIMER_LEFT_2=59,22
PRIMER_RIGHT_2=204,21
PRIMER_LEFT_2_TM=60.302
PRIMER_RIGHT_2_TM=60.206
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=11.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=1.5081
PRIMER_LEFT_3_PENALTY=0.301926
PRIMER_RIGHT_3_PENALTY=1.206212
PRIMER_LEFT_3_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_3_SEQUENCE=ATATAATACACGATGGCCGCA
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=204,21
PRIMER_LEFT_3_TM=60.302
PRIMER_RIGHT_3_TM=60.206
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=11.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=1.5383
PRIMER_LEFT_4_PENALTY=0.589035
PRIMER_RIGHT_4_PENALTY=0.949261
PRIMER_LEFT_4_SEQUENCE=GAGGAAGCTACTTTCTTGGTGG
PRIMER_RIGHT_4_SEQUENCE=ATATAATACACGATGGCCGCAC
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=204,22
PRIMER_LEFT_4_TM=59.411
PRIMER_RIGHT_4_TM=60.949
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_02679 Ps_contig_mira-and-tgicl-ass_franssen_23740
SEQUENCE=TCGGGGACATTCGGCCCCCACAAAACCCTTTCAAATTCACCTCTCTCTCTCTCTCTCTCTTCCGTATTTTCGTTTTCTAGGTCTATTTCTAATTTCAACGAAAATGAGAAGAAATTCTTCATA
TARGET=41,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02680 Ps_contig_mira-and-tgicl-ass_franssen_23756
SEQUENCE=GTCGGGGKACCATTGATAAGRATACAATTATCAAAAGCATAACAATCTTTTCAATTTCATTGCAATATACAYACACGATGGCCGCATCATCATCATCATCCATGGCCCTCTCTTCTCCAACCTTGGCTGGCAAGCAACTCAAGCTGAMCCCATCAAGCCAAGAATTGGGAGCTGCAAGGTTCACCATGAGGAAGTCTGCTACCACCAAGAAAG
TARGET=85,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02681 Ps_contig_mira-and-tgicl-ass_franssen_23782
SEQUENCE=TAGCCGCCGGCAGCTACGCAGCCGCTTTTTTTAGTTTTTCCGTTTTACTTTAGCAAATTCAAACAACACCCTCGACGCAATAACCGCCGACTTCGACAAAATCGAACAACTTTTCTCTGACCCCAAGGTGTTCGACTATTTCTCCAGCCCAATAGTCGAAGATTCCACCAAAACGCCAACTTATAGGAGAGTTCGCCACTACCTCAGGCTTCCAACCACACACACACAACTTCCTTAACGTCTTGATCGACTCGAAGAGGATCGACATGATCATCGACATTAWTAAGGNGTTTGAATTTGTTTACAATACTTTACTGNTACAGAGCTTGTGGTTGTGACTTCGGTTGTGAAGTTGGAGTCAACATCTATTTGGCTCAGGATTTGACAAAACAGGTTTCAGAAGCTTACGTGGGGGACAAAGAAAGTGAACGG
TARGET=217,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02682 Ps_contig_mira-and-tgicl-ass_franssen_23783
SEQUENCE=TGTCAGCTAGGGACTTAACTGAGACTTCAACTAATACCAAAGATGACGTTGTTGAAAAGTCAGATGAATTAAATGATGCCAAATATTACGGTGGAGGTTACAACCATGGTGGTCGGCGGAGGAGGCTACAACCATGGTGGTGGTGGCTACAATGGTGGTGGAGGAGGATACAACCATGGAGGTGGTGGTTACAATGGTGGCGGAGGAGGATACAACCATGGAGGCGGTGGTTACAACGGTGGCGGTGGAGGATACAACCATGGTGGCGGAGGAGGATACAACCATGGTGGAGGTGGTTACAATGGCGGTGGAGGTTACCATAATGGTGGTGGTGGTTACCATGGTGGAGGTGGACACGGTGGACATGGTGGTGCTTCCAACAACAGTAACTGATAATATCAACATTCTCAGTAATAAATATCATGAATTAATAATGTTGGATGTTGAAAAAAAAGAAGAGGTATAGATAGACATGAATACTTTTATTGGTTATGTATTATCTATTCTTTGCAACCTTACTTTAGTTATTAGTTAGTTTCTCACTCTATAAATA
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5410
PRIMER_LEFT_PENALTY=0.565974
PRIMER_RIGHT_PENALTY=0.975014
PRIMER_LEFT_SEQUENCE=GATGCCAAATATTACGGTGGAG
PRIMER_RIGHT_SEQUENCE=ACCTCCATGGTTGTATCCTCCT
PRIMER_LEFT=74,22
PRIMER_RIGHT=182,22
PRIMER_LEFT_TM=60.566
PRIMER_RIGHT_TM=60.975
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=1.7071
PRIMER_LEFT_1_PENALTY=0.565974
PRIMER_RIGHT_1_PENALTY=1.141149
PRIMER_LEFT_1_SEQUENCE=GATGCCAAATATTACGGTGGAG
PRIMER_RIGHT_1_SEQUENCE=CACCATTGTAACCACCACCTC
PRIMER_LEFT_1=74,22
PRIMER_RIGHT_1=198,21
PRIMER_LEFT_1_TM=60.566
PRIMER_RIGHT_1_TM=60.141
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=1.7632
PRIMER_LEFT_2_PENALTY=0.565974
PRIMER_RIGHT_2_PENALTY=1.197222
PRIMER_LEFT_2_SEQUENCE=GATGCCAAATATTACGGTGGAG
PRIMER_RIGHT_2_SEQUENCE=CCTCCATGGTTGTATCCTCCT
PRIMER_LEFT_2=74,22
PRIMER_RIGHT_2=181,21
PRIMER_LEFT_2_TM=60.566
PRIMER_RIGHT_2_TM=60.197
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=1.7632
PRIMER_LEFT_3_PENALTY=0.565974
PRIMER_RIGHT_3_PENALTY=1.197222
PRIMER_LEFT_3_SEQUENCE=GATGCCAAATATTACGGTGGAG
PRIMER_RIGHT_3_SEQUENCE=CCTCCATGGTTGTATCCTCCT
PRIMER_LEFT_3=74,22
PRIMER_RIGHT_3=223,21
PRIMER_LEFT_3_TM=60.566
PRIMER_RIGHT_3_TM=60.197
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=150
PRIMER_PAIR_PENALTY_4=1.7726
PRIMER_LEFT_4_PENALTY=0.797580
PRIMER_RIGHT_4_PENALTY=0.975014
PRIMER_LEFT_4_SEQUENCE=ATGATGCCAAATATTACGGTGG
PRIMER_RIGHT_4_SEQUENCE=ACCTCCATGGTTGTATCCTCCT
PRIMER_LEFT_4=72,22
PRIMER_RIGHT_4=182,22
PRIMER_LEFT_4_TM=60.798
PRIMER_RIGHT_4_TM=60.975
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=111
=
PRIMER_SEQUENCE_ID=SSR_02683 Ps_contig_mira-and-tgicl-ass_franssen_23783
SEQUENCE=TGTCAGCTAGGGACTTAACTGAGACTTCAACTAATACCAAAGATGACGTTGTTGAAAAGTCAGATGAATTAAATGATGCCAAATATTACGGTGGAGGTTACAACCATGGTGGTCGGCGGAGGAGGCTACAACCATGGTGGTGGTGGCTACAATGGTGGTGGAGGAGGATACAACCATGGAGGTGGTGGTTACAATGGTGGCGGAGGAGGATACAACCATGGAGGCGGTGGTTACAACGGTGGCGGTGGAGGATACAACCATGGTGGCGGAGGAGGATACAACCATGGTGGAGGTGGTTACAATGGCGGTGGAGGTTACCATAATGGTGGTGGTGGTTACCATGGTGGAGGTGGACACGGTGGACATGGTGGTGCTTCCAACAACAGTAACTGATAATATCAACATTCTCAGTAATAAATATCATGAATTAATAATGTTGGATGTTGAAAAAAAAGAAGAGGTATAGATAGACATGAATACTTTTATTGGTTATGTATTATCTATTCTTTGCAACCTTACTTTAGTTATTAGTTAGTTTCTCACTCTATAAATA
TARGET=324,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1405
PRIMER_LEFT_PENALTY=1.066501
PRIMER_RIGHT_PENALTY=0.073989
PRIMER_LEFT_SEQUENCE=AATGGTGGTGGAGGAGGATAC
PRIMER_RIGHT_SEQUENCE=TTACTGTTGTTGGAAGCACCAC
PRIMER_LEFT=150,21
PRIMER_RIGHT=388,22
PRIMER_LEFT_TM=60.067
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.3000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=1.1405
PRIMER_LEFT_1_PENALTY=1.066501
PRIMER_RIGHT_1_PENALTY=0.073989
PRIMER_LEFT_1_SEQUENCE=GGTGGAGGAGGATACAACCAT
PRIMER_RIGHT_1_SEQUENCE=TTACTGTTGTTGGAAGCACCAC
PRIMER_LEFT_1=156,21
PRIMER_RIGHT_1=388,22
PRIMER_LEFT_1_TM=60.067
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=1.6966
PRIMER_LEFT_2_PENALTY=1.622602
PRIMER_RIGHT_2_PENALTY=0.073989
PRIMER_LEFT_2_SEQUENCE=GTGGTGGAGGAGGATACAACC
PRIMER_RIGHT_2_SEQUENCE=TTACTGTTGTTGGAAGCACCAC
PRIMER_LEFT_2=154,21
PRIMER_RIGHT_2=388,22
PRIMER_LEFT_2_TM=60.623
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=235
PRIMER_PAIR_PENALTY_3=2.0346
PRIMER_LEFT_3_PENALTY=1.960583
PRIMER_RIGHT_3_PENALTY=0.073989
PRIMER_LEFT_3_SEQUENCE=GGAGGAGGATACAACCATGGAG
PRIMER_RIGHT_3_SEQUENCE=TTACTGTTGTTGGAAGCACCAC
PRIMER_LEFT_3=201,22
PRIMER_RIGHT_3=388,22
PRIMER_LEFT_3_TM=61.961
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=2.0346
PRIMER_LEFT_4_PENALTY=1.960583
PRIMER_RIGHT_4_PENALTY=0.073989
PRIMER_LEFT_4_SEQUENCE=GGAGGAGGATACAACCATGGAG
PRIMER_RIGHT_4_SEQUENCE=TTACTGTTGTTGGAAGCACCAC
PRIMER_LEFT_4=159,22
PRIMER_RIGHT_4=388,22
PRIMER_LEFT_4_TM=61.961
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_02684 Ps_contig_mira-and-tgicl-ass_franssen_23792
SEQUENCE=GAATGCGTGTCATTATGTGTAGTAGTGAACTAGTATAAAGTGCTCTCTCATCTCTTCGTTTCGATCATCCAAACAACAACAACACTATCATCATCGTCTCATTACCTTTTACCTTTCATTTCAGAGTTACAGCTACATATCTATTTAGTATATTAATTATTCCAGTTCGACTCCATTC
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8573
PRIMER_LEFT_PENALTY=0.044832
PRIMER_RIGHT_PENALTY=1.812474
PRIMER_LEFT_SEQUENCE=TCTCATCTCTTCGTTTCGATCA
PRIMER_RIGHT_SEQUENCE=GAATGGAGTCGAACTGGAATAA
PRIMER_LEFT=45,22
PRIMER_RIGHT=177,22
PRIMER_LEFT_TM=59.955
PRIMER_RIGHT_TM=58.188
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=2.4625
PRIMER_LEFT_1_PENALTY=0.650009
PRIMER_RIGHT_1_PENALTY=1.812474
PRIMER_LEFT_1_SEQUENCE=GCTCTCTCATCTCTTCGTTTCG
PRIMER_RIGHT_1_SEQUENCE=GAATGGAGTCGAACTGGAATAA
PRIMER_LEFT_1=41,22
PRIMER_RIGHT_1=177,22
PRIMER_LEFT_1_TM=60.650
PRIMER_RIGHT_1_TM=58.188
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.0000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=2.7715
PRIMER_LEFT_2_PENALTY=0.959072
PRIMER_RIGHT_2_PENALTY=1.812474
PRIMER_LEFT_2_SEQUENCE=CATCTCTTCGTTTCGATCATCC
PRIMER_RIGHT_2_SEQUENCE=GAATGGAGTCGAACTGGAATAA
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=177,22
PRIMER_LEFT_2_TM=60.959
PRIMER_RIGHT_2_TM=58.188
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=130
PRIMER_PAIR_PENALTY_3=2.9954
PRIMER_LEFT_3_PENALTY=1.182928
PRIMER_RIGHT_3_PENALTY=1.812474
PRIMER_LEFT_3_SEQUENCE=TGCTCTCTCATCTCTTCGTTTC
PRIMER_RIGHT_3_SEQUENCE=GAATGGAGTCGAACTGGAATAA
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=177,22
PRIMER_LEFT_3_TM=58.817
PRIMER_RIGHT_3_TM=58.188
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=3.1552
PRIMER_LEFT_4_PENALTY=1.342747
PRIMER_RIGHT_4_PENALTY=1.812474
PRIMER_LEFT_4_SEQUENCE=GTGCTCTCTCATCTCTTCGTTTC
PRIMER_RIGHT_4_SEQUENCE=GAATGGAGTCGAACTGGAATAA
PRIMER_LEFT_4=39,23
PRIMER_RIGHT_4=177,22
PRIMER_LEFT_4_TM=59.657
PRIMER_RIGHT_4_TM=58.188
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_02685 Ps_contig_mira-and-tgicl-ass_franssen_23847
SEQUENCE=TCCGACCACCAACGACCACGTACCTACCTACCTTCCCTTCGTCCTTCCTCTACTTCCTTCCTTACCGTACCTACTTACCCTCTACTTCTTCTTTCTTNTNTTTCCTTTCTTCTTCTTTCTTTTCTCACCATCTTCTCCTTCCTCTTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGTAAGTTTCTCCAAGAGACTGGTNTGTTTTCTTTCCACCTTTCCTCTTTACTAACTTTTCGGGTTTCCCG
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02686 Ps_contig_mira-and-tgicl-ass_franssen_23865
SEQUENCE=TTCGGGGGTACTTCAATWCTAAACACAAACACAGTCTCTCTCTCTTTTCACCTATCGCGTCCCTCTTTCTCTCGGGCTCTCACTGCGTCAAAAACCNCGAGAAGGAGGAACAACATGCAGATCCTTCGTCAAAGTACCTTTAAACCCGGAAAAACCNTCACTCTCGAGGTTGAGAGCAGCGACACCTYCGAACAACGTCAAGGCCAAGNTCCAGGACAAGGAGTNTCCCTCTGACCAGCAGTAGG
TARGET=35,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02687 Ps_contig_mira-and-tgicl-ass_franssen_23876
SEQUENCE=ATCATTTTGGCTTCTTTATCCATTCCAATTATTCAGAACGTGGAATTGAAAGCAAGAGGACAAAAACTTCAACAGGGACCAGTAGACAACAACAACAACAACATCAACAACTCATAACAATAATTGAGGCCAAACATGTTGCATTCATTAAGCATTGCTCCTGAAGGATCCAAGAGCTTTAATTGCTGATCCTCTTAGAATGTATTGGTGGTAAATTGCAGCCACTGCAGCAACCAAATAAATCGGTACCCAAACCCAAATAAACTCCACTGGTCACTCCCAAGTCTTTGTCGTTGTWGAAGATTAACGG
TARGET=86,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02688 Ps_contig_mira-and-tgicl-ass_franssen_23877
SEQUENCE=TAACCATAATTAACAATAATAAACTTTAAATTATTAGGAAAGGAAACGTAAAGTAAAATAAATTTAAATTAGAAAAGACTTTTNAACCTTGATGACATGCTAAAATGGTAGACCCGATCCCTAACATCTAGAACCATTAATTTAAACTTCCGTTTTTTACCAGTTCCAGTCACACCCATTCCATTGGTTTAAGCTTCAGGATTCTCAACTGGAACTTCCTCTGCTGCTGGAGCTTCCACTGATTCAACTTGTTTTGTTCTCAATCACATCCACTGCAGTTGCTGGTGGTGGTGGTNCTGACTCTATTTTCAGGTAGCTGCTATTCGGCCTTCCCTCCACTTACGTCGTCCTTCCCGT
TARGET=283,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7493
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.729458
PRIMER_LEFT_SEQUENCE=ACACCCATTCCATTGGTTTAAG
PRIMER_RIGHT_SEQUENCE=CCGAATAGCAGCTACCTGAAAA
PRIMER_LEFT=171,22
PRIMER_RIGHT=326,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.729
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=0.7822
PRIMER_LEFT_1_PENALTY=0.052729
PRIMER_RIGHT_1_PENALTY=0.729458
PRIMER_LEFT_1_SEQUENCE=CTCAACTGGAACTTCCTCTGCT
PRIMER_RIGHT_1_SEQUENCE=CCGAATAGCAGCTACCTGAAAA
PRIMER_LEFT_1=203,22
PRIMER_RIGHT_1=326,22
PRIMER_LEFT_1_TM=60.053
PRIMER_RIGHT_1_TM=60.729
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.8439
PRIMER_LEFT_2_PENALTY=0.114470
PRIMER_RIGHT_2_PENALTY=0.729458
PRIMER_LEFT_2_SEQUENCE=AAGCTTCAGGATTCTCAACTGG
PRIMER_RIGHT_2_SEQUENCE=CCGAATAGCAGCTACCTGAAAA
PRIMER_LEFT_2=190,22
PRIMER_RIGHT_2=326,22
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=60.729
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=0.9860
PRIMER_LEFT_3_PENALTY=0.256503
PRIMER_RIGHT_3_PENALTY=0.729458
PRIMER_LEFT_3_SEQUENCE=ACATGCTAAAATGGTAGACCCG
PRIMER_RIGHT_3_SEQUENCE=CCGAATAGCAGCTACCTGAAAA
PRIMER_LEFT_3=94,22
PRIMER_RIGHT_3=326,22
PRIMER_LEFT_3_TM=60.257
PRIMER_RIGHT_3_TM=60.729
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.1000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=1.1117
PRIMER_LEFT_4_PENALTY=0.382228
PRIMER_RIGHT_4_PENALTY=0.729458
PRIMER_LEFT_4_SEQUENCE=AGCTTCAGGATTCTCAACTGGA
PRIMER_RIGHT_4_SEQUENCE=CCGAATAGCAGCTACCTGAAAA
PRIMER_LEFT_4=191,22
PRIMER_RIGHT_4=326,22
PRIMER_LEFT_4_TM=60.382
PRIMER_RIGHT_4_TM=60.729
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=136
=
PRIMER_SEQUENCE_ID=SSR_02689 Ps_contig_mira-and-tgicl-ass_franssen_23889
SEQUENCE=ACACAAAAAAAACACACCAAGCCAGTGAAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAGGCTTGCTTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATTGTTACAACATCCACTTCGAACCCCTTTAAACCAAAGAAGTAGTTTCTAGCTTGTTACCAAACCATACGTACCAAAAACAACTACCCCATCTACAAAACAACAGA
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2959
PRIMER_LEFT_PENALTY=1.200250
PRIMER_RIGHT_PENALTY=0.095684
PRIMER_LEFT_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_SEQUENCE=ACAATGCAACGGTGTAACTGAG
PRIMER_LEFT=9,21
PRIMER_RIGHT=139,22
PRIMER_LEFT_TM=60.200
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=131
PRIMER_PAIR_PENALTY_1=1.3249
PRIMER_LEFT_1_PENALTY=1.200250
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_1_SEQUENCE=CAACTGCATGAAATGAGAGGAG
PRIMER_LEFT_1=9,21
PRIMER_RIGHT_1=116,22
PRIMER_LEFT_1_TM=60.200
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=108
PRIMER_PAIR_PENALTY_2=1.4035
PRIMER_LEFT_2_PENALTY=1.200250
PRIMER_RIGHT_2_PENALTY=0.203283
PRIMER_LEFT_2_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_2_SEQUENCE=GATGTTGTAACAATGCAACGGT
PRIMER_LEFT_2=9,21
PRIMER_RIGHT_2=148,22
PRIMER_LEFT_2_TM=60.200
PRIMER_RIGHT_2_TM=59.797
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=1.4600
PRIMER_LEFT_3_PENALTY=1.200250
PRIMER_RIGHT_3_PENALTY=0.259704
PRIMER_LEFT_3_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_3_SEQUENCE=ACAACTGCATGAAATGAGAGGA
PRIMER_LEFT_3=9,21
PRIMER_RIGHT_3=117,22
PRIMER_LEFT_3_TM=60.200
PRIMER_RIGHT_3_TM=59.740
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=1.4600
PRIMER_LEFT_4_PENALTY=1.200250
PRIMER_RIGHT_4_PENALTY=0.259704
PRIMER_LEFT_4_SEQUENCE=AAACACACCAAGCCAGTGAAG
PRIMER_RIGHT_4_SEQUENCE=GACAACTGCATGAAATGAGAGG
PRIMER_LEFT_4=9,21
PRIMER_RIGHT_4=118,22
PRIMER_LEFT_4_TM=60.200
PRIMER_RIGHT_4_TM=59.740
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_02690 Ps_contig_mira-and-tgicl-ass_franssen_23896
SEQUENCE=AGTTTTTTCCTTAAAACGTAAAGAAATAATACTATTAATTTTTTAATTTTAATTTTAAAAGTTTTAACTTAACCAAACTTAACTAAACCAACTAACATCACACATGGGCGTGGGCCGAGATTCCAACTTAAAAGTAAATAGAAAAAACTAAGCCCCACTCACCCAACGCACTCACCACCTAATAATAATAATCATTACAACAACACGTTACACTTACAACAACATCCTTTACATACAAAAAGGGAAATAGTTAAAACAAGATAATGATCGAATTTCAGATTAAAAATAAAMCCACAAGTGGTTGGTTTATGTTTGGCATCACTATCNCAACCGTTGGNTCNTATCACTTGCTNAAGATGCTCTNCGTTGNTACACTGNT
TARGET=180,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02691 Ps_contig_mira-and-tgicl-ass_franssen_23899
SEQUENCE=TTCGGGGGTTKGGTAAAGAGGTTGTTGTTGTGGGGATAAGATTGCAGTGAGTGAGTGAGTTTGTGAAACTATGGCTTCTGCTTCAGCATTCTTATTTTCAAATCATCATCACTTGTTCTTGATAAGTCTGAATGGGTAGAAGGAAAACTCTCCTCCGTCACCTTCTTCTATGTCTGTTGCCAGATGCCAACCCTGCTAATTCATCTAACGTTTACCACTCCGACGCGTTCGGTTCCTTACG
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02692 Ps_contig_mira-and-tgicl-ass_franssen_23903
SEQUENCE=TCGGGGKACTATCTTTTTCATACTATATCATATAATTCCATAACCAACCTTCTCTTCCATTCCATTCCATTCACTCATCAAACCAAAAACAATGGCAGCATCAGCATCATCCATGATCTCAACAAACGCCCTAACATCCACACTCCAAAAACACCAAACACACAACCTCAAACCATCAAACCTATGTTTCCAAGGCTTCAAACCCTTAACAAGATCAACAAAA
TARGET=55,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02693 Ps_contig_mira-and-tgicl-ass_franssen_23908
SEQUENCE=GATTCCATTCACTCATCAAACCAAAAACAATGGCAGCATCAGCATCATCCATGATCTCAACAAACGCCCTAACATCCACACTCCAAAAACAACCAAAACAACAACAACCTCAAACCATCAAACCTATGTTTCCAAGGCTTCAAACCCTTAACAAGATCAACAAAACTGACACAGAACAAAAAGAGNTTCTCATCCTTGTTGTCAAAGCTGAACTCAACCCTCTCA
TARGET=97,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1880
PRIMER_LEFT_PENALTY=0.101680
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=ATCTCAACAAACGCCCTAACAT
PRIMER_RIGHT_SEQUENCE=CAGCTTTGACAACAAGGATGAG
PRIMER_LEFT=53,22
PRIMER_RIGHT=209,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.3906
PRIMER_LEFT_1_PENALTY=0.101680
PRIMER_RIGHT_1_PENALTY=0.288917
PRIMER_LEFT_1_SEQUENCE=ATCTCAACAAACGCCCTAACAT
PRIMER_RIGHT_1_SEQUENCE=GAGGGTTGAGTTCAGCTTTGAC
PRIMER_LEFT_1=53,22
PRIMER_RIGHT_1=221,22
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=60.289
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.5169
PRIMER_LEFT_2_PENALTY=0.101680
PRIMER_RIGHT_2_PENALTY=0.415247
PRIMER_LEFT_2_SEQUENCE=ATCTCAACAAACGCCCTAACAT
PRIMER_RIGHT_2_SEQUENCE=GAGAGGGTTGAGTTCAGCTTTG
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=223,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.415
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.5169
PRIMER_LEFT_3_PENALTY=0.101680
PRIMER_RIGHT_3_PENALTY=0.415247
PRIMER_LEFT_3_SEQUENCE=ATCTCAACAAACGCCCTAACAT
PRIMER_RIGHT_3_SEQUENCE=AGAGGGTTGAGTTCAGCTTTGA
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=222,22
PRIMER_LEFT_3_TM=59.898
PRIMER_RIGHT_3_TM=60.415
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=0.5169
PRIMER_LEFT_4_PENALTY=0.101680
PRIMER_RIGHT_4_PENALTY=0.415247
PRIMER_LEFT_4_SEQUENCE=ATCTCAACAAACGCCCTAACAT
PRIMER_RIGHT_4_SEQUENCE=TGAGAGGGTTGAGTTCAGCTTT
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=224,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=60.415
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_02694 Ps_contig_mira-and-tgicl-ass_franssen_23924
SEQUENCE=AAAGGGAACTAAAACAATATGGTTTATGATTATTGTTAATTGATGCTTCTAGGTTTCCTGTTACTCCTCTTGAGTGTCTTCATCATCGTCATAGATTATTCTAATCATGTCAAACTTGTGTATTATTATTATGGCTGGAAATGGTGAATGTTGGTGCAAGAAATGTTCCACTTGTGTCATATTATTGTATGATATTTATTGGCAAAACTNTATATAATGTGGARAKWAMKTAGWARSTAAAGTTAGGTATAGTTAAAACGAAACTAAAGTTGTATTATTATAGGTGGGAATAAGTGANATTGTT
TARGET=121,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02695 Ps_contig_mira-and-tgicl-ass_franssen_23926
SEQUENCE=TAACCGGTGGAGGAGGTAGATTTATATTTGAATTGGNGTGAATAGACAGGTGGAGGAGGAGTTTTACTTTTATATTGTAACGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGGGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATTATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTNACTTGTATTTGTAAACTGGGTGGTGGAGGAAGAAGGATTTA
TARGET=200,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3173
PRIMER_LEFT_PENALTY=0.173354
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_SEQUENCE=CTCCACCACCCAGTTTACAAAT
PRIMER_LEFT=167,22
PRIMER_RIGHT=353,22
PRIMER_LEFT_TM=59.827
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.4533
PRIMER_LEFT_1_PENALTY=0.309369
PRIMER_RIGHT_1_PENALTY=0.143968
PRIMER_LEFT_1_SEQUENCE=TAGACACGTGGAGGAGGAGACT
PRIMER_RIGHT_1_SEQUENCE=CTCCACCACCCAGTTTACAAAT
PRIMER_LEFT_1=134,22
PRIMER_RIGHT_1=353,22
PRIMER_LEFT_1_TM=60.309
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=220
PRIMER_PAIR_PENALTY_2=0.4884
PRIMER_LEFT_2_PENALTY=0.005574
PRIMER_RIGHT_2_PENALTY=0.482784
PRIMER_LEFT_2_SEQUENCE=GTGAATAGACAGGTGGAGGAGG
PRIMER_RIGHT_2_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=318,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.6561
PRIMER_LEFT_3_PENALTY=0.173354
PRIMER_RIGHT_3_PENALTY=0.482784
PRIMER_LEFT_3_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_3_SEQUENCE=CCTCCACCGGTATACAATTCAC
PRIMER_LEFT_3=167,22
PRIMER_RIGHT_3=320,22
PRIMER_LEFT_3_TM=59.827
PRIMER_RIGHT_3_TM=60.483
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.6561
PRIMER_LEFT_4_PENALTY=0.173354
PRIMER_RIGHT_4_PENALTY=0.482784
PRIMER_LEFT_4_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_4_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_4=167,22
PRIMER_RIGHT_4=318,22
PRIMER_LEFT_4_TM=59.827
PRIMER_RIGHT_4_TM=60.483
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_02696 Ps_contig_mira-and-tgicl-ass_franssen_23926
SEQUENCE=TAACCGGTGGAGGAGGTAGATTTATATTTGAATTGGNGTGAATAGACAGGTGGAGGAGGAGTTTTACTTTTATATTGTAACGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGGGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATTATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTNACTTGTATTTGTAAACTGGGTGGTGGAGGAAGAAGGATTTA
TARGET=231,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3173
PRIMER_LEFT_PENALTY=0.173354
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_SEQUENCE=CTCCACCACCCAGTTTACAAAT
PRIMER_LEFT=167,22
PRIMER_RIGHT=353,22
PRIMER_LEFT_TM=59.827
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.4533
PRIMER_LEFT_1_PENALTY=0.309369
PRIMER_RIGHT_1_PENALTY=0.143968
PRIMER_LEFT_1_SEQUENCE=TAGACACGTGGAGGAGGAGACT
PRIMER_RIGHT_1_SEQUENCE=CTCCACCACCCAGTTTACAAAT
PRIMER_LEFT_1=134,22
PRIMER_RIGHT_1=353,22
PRIMER_LEFT_1_TM=60.309
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=220
PRIMER_PAIR_PENALTY_2=0.4884
PRIMER_LEFT_2_PENALTY=0.005574
PRIMER_RIGHT_2_PENALTY=0.482784
PRIMER_LEFT_2_SEQUENCE=GTGAATAGACAGGTGGAGGAGG
PRIMER_RIGHT_2_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=318,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.6561
PRIMER_LEFT_3_PENALTY=0.173354
PRIMER_RIGHT_3_PENALTY=0.482784
PRIMER_LEFT_3_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_3_SEQUENCE=CCTCCACCGGTATACAATTCAC
PRIMER_LEFT_3=167,22
PRIMER_RIGHT_3=320,22
PRIMER_LEFT_3_TM=59.827
PRIMER_RIGHT_3_TM=60.483
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.6561
PRIMER_LEFT_4_PENALTY=0.173354
PRIMER_RIGHT_4_PENALTY=0.482784
PRIMER_LEFT_4_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_4_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_4=167,22
PRIMER_RIGHT_4=318,22
PRIMER_LEFT_4_TM=59.827
PRIMER_RIGHT_4_TM=60.483
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_02697 Ps_contig_mira-and-tgicl-ass_franssen_23929
SEQUENCE=GGATGGTATATCAGGTCACAGCAGTTGACAGTGGCAGTAGCAGTGATGAATGTGACAGCGACAACGAAACCGTCGCCACAGGAAGTACTGCAACTGAGAAGCTGCTGACATAATAATAATAAGTGGTAGTTGAGTATTGTAGTTTGGNGTAGTATTGACGTAGGTAAAGTTTGAAATTAAAGTTACGGACCGTTTAAAACGGTTTAAAAGTAACTTAAAAACCGTTGGTAAACGAAAACCTAAAAAAAA
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5396
PRIMER_LEFT_PENALTY=0.347541
PRIMER_RIGHT_PENALTY=0.192103
PRIMER_LEFT_SEQUENCE=CCACAGGAAGTACTGCAACTGA
PRIMER_RIGHT_SEQUENCE=TAGGTTTTCGTTTACCAACGGT
PRIMER_LEFT=75,22
PRIMER_RIGHT=241,22
PRIMER_LEFT_TM=60.348
PRIMER_RIGHT_TM=59.808
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.5579
PRIMER_LEFT_1_PENALTY=0.365769
PRIMER_RIGHT_1_PENALTY=0.192103
PRIMER_LEFT_1_SEQUENCE=ATCAGGTCACAGCAGTTGACAG
PRIMER_RIGHT_1_SEQUENCE=TAGGTTTTCGTTTACCAACGGT
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=241,22
PRIMER_LEFT_1_TM=60.366
PRIMER_RIGHT_1_TM=59.808
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.6039
PRIMER_LEFT_2_PENALTY=0.347541
PRIMER_RIGHT_2_PENALTY=0.256359
PRIMER_LEFT_2_SEQUENCE=CCACAGGAAGTACTGCAACTGA
PRIMER_RIGHT_2_SEQUENCE=TTAGGTTTTCGTTTACCAACGG
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=242,22
PRIMER_LEFT_2_TM=60.348
PRIMER_RIGHT_2_TM=60.256
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.6221
PRIMER_LEFT_3_PENALTY=0.365769
PRIMER_RIGHT_3_PENALTY=0.256359
PRIMER_LEFT_3_SEQUENCE=ATCAGGTCACAGCAGTTGACAG
PRIMER_RIGHT_3_SEQUENCE=TTAGGTTTTCGTTTACCAACGG
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=242,22
PRIMER_LEFT_3_TM=60.366
PRIMER_RIGHT_3_TM=60.256
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.9117
PRIMER_LEFT_4_PENALTY=0.719637
PRIMER_RIGHT_4_PENALTY=0.192103
PRIMER_LEFT_4_SEQUENCE=GGCAGTAGCAGTGATGAATGTG
PRIMER_RIGHT_4_SEQUENCE=TAGGTTTTCGTTTACCAACGGT
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=241,22
PRIMER_LEFT_4_TM=60.720
PRIMER_RIGHT_4_TM=59.808
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_02698 Ps_contig_mira-and-tgicl-ass_franssen_23932
SEQUENCE=YGCTGGTGGTGGTGGTGCTGACTCTATTTTTCAGTAGTTGCTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTTCACTTCTTCAACCGATTCCTCAGTTTCTTTAGGTGCTTCTAAGTTCTCTTCCTTTGCTTCCTCTGTTACCACTTCCTTGTTCTCAACTTCAAGTGGATCTTAGGGTTWCGGRAGCATCTACAGCTTCTCCTGCCYGCTAGTTTGTTTCTGTTTGGTTTTTCGGTNTTCTNTGTTGTACTTGGNGCAGGACTTCGG
TARGET=4,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02699 Ps_contig_mira-and-tgicl-ass_franssen_23936
SEQUENCE=GAAACAAACAAACACAAAGCATACAATACAAGAAGAGACATTATTGCAAATGTGGGTAGACAAGATGCCAAGAAGCACCTCGAAGAACATGTCTCCAATTTGATGTCTTCTAACATCGTTCAAACTCTTGGAATGRTGCTTGACACTGTTGTCTTCTAGAGTTGTTATTTTGGACCTTCTGGTTAATCTAGCATGTTAAGGAGACAAGTTATTGTTTACAATTTGGGTTTCCTAATTATTCTCAGACTATATTTGAATACAGATGTATTCTTTGGATGAGCTGAAAAGGTCAAAGAAAGTWAAACATAGCACGTWAGTTTAACTTARAWMGTT
TARGET=2,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02700 Ps_contig_mira-and-tgicl-ass_franssen_23941
SEQUENCE=AGTTGAAGAGGAAGGTGAAAGAAACACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTCAAGTGAAGAGGAAGGAGAAGATGGTGAGAAAAGAAAGAAGAAGAAGGAAANAGAAGAAAGAAGAAAACTACGGTGCCAGTTGAGAAAGTAGAGGTGTGTTGAAGGAACAACCGTACCGAGGAAAGAAG
TARGET=112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0765
PRIMER_LEFT_PENALTY=0.012411
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=CCTCAAGTGAAGAGGAAGGAGA
PRIMER_RIGHT_SEQUENCE=GTACGGTTGTTCCTTCAACACA
PRIMER_LEFT=73,22
PRIMER_RIGHT=192,22
PRIMER_LEFT_TM=59.988
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=0.0765
PRIMER_LEFT_1_PENALTY=0.012411
PRIMER_RIGHT_1_PENALTY=0.064105
PRIMER_LEFT_1_SEQUENCE=TCCTCAAGTGAAGAGGAAGGAG
PRIMER_RIGHT_1_SEQUENCE=GTACGGTTGTTCCTTCAACACA
PRIMER_LEFT_1=72,22
PRIMER_RIGHT_1=192,22
PRIMER_LEFT_1_TM=59.988
PRIMER_RIGHT_1_TM=59.936
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=121
PRIMER_PAIR_PENALTY_2=0.1816
PRIMER_LEFT_2_PENALTY=0.117452
PRIMER_RIGHT_2_PENALTY=0.064105
PRIMER_LEFT_2_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_RIGHT_2_SEQUENCE=GTACGGTTGTTCCTTCAACACA
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=192,22
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=59.936
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.1816
PRIMER_LEFT_3_PENALTY=0.117452
PRIMER_RIGHT_3_PENALTY=0.064105
PRIMER_LEFT_3_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_RIGHT_3_SEQUENCE=TACGGTTGTTCCTTCAACACAC
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=191,22
PRIMER_LEFT_3_TM=60.117
PRIMER_RIGHT_3_TM=59.936
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.2468
PRIMER_LEFT_4_PENALTY=0.182682
PRIMER_RIGHT_4_PENALTY=0.064105
PRIMER_LEFT_4_SEQUENCE=GAGGAAGGAGAAGATGGTGAGA
PRIMER_RIGHT_4_SEQUENCE=GTACGGTTGTTCCTTCAACACA
PRIMER_LEFT_4=84,22
PRIMER_RIGHT_4=192,22
PRIMER_LEFT_4_TM=59.817
PRIMER_RIGHT_4_TM=59.936
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_02701 Ps_contig_mira-and-tgicl-ass_franssen_23949
SEQUENCE=CGTGTTGGAAGTTGAAAGGATTGGTGTTTACCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAAKTATCTCTTATGTGTTGTGWGTTYYTTTTTTACTGTGTACACTTCTACCAAG
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02702 Ps_contig_mira-and-tgicl-ass_franssen_23953
SEQUENCE=CGGGGKAGGTGGCAACGGGAAGACACCTGGTGGGGGAGGTGGTGGAGGACCTATGCCGGTTCAAATTCATGGTATGGTTGGTGGAAATGGCAACGGGGGTAAGAATGGCGGGGGTGGTGGTGGAGGGAACAAAATGAATGGTGGTTTACCGGAAGGACTAAAACTGGTGGTGGTGGTGGTGGTGGTGGACATGGTGGTGCTTCCGACAATGGTAACTGATGATACCATGATAAATGATGCACATTCAAAGTAATTAAG
TARGET=165,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02703 Ps_contig_mira-and-tgicl-ass_franssen_23967
SEQUENCE=GTCGGGGTACCATTGATAAGGATACAATTATCAAAAGCATAACAATCTTTTCAATTTCATTGCAATATAATACACGATGGCCGCATCATCATCATCATCCATGGCTCTCTCTTCTCCAACCTTGGCTGGCAAGCAACTCAAGCTGAACCCATCAAGCCAAGAAGTTGGGAGCTGACAAGGTTCACCATGAGGAGTCTGCCTACCCACCAAAG
TARGET=84,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7439
PRIMER_LEFT_PENALTY=1.083165
PRIMER_RIGHT_PENALTY=0.660713
PRIMER_LEFT_SEQUENCE=CGGGGTACCATTGATAAGGAT
PRIMER_RIGHT_SEQUENCE=CTTGATGGGTTCAGCTTGAGTT
PRIMER_LEFT=2,21
PRIMER_RIGHT=155,22
PRIMER_LEFT_TM=59.917
PRIMER_RIGHT_TM=60.661
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=1.8056
PRIMER_LEFT_1_PENALTY=1.391547
PRIMER_RIGHT_1_PENALTY=0.414033
PRIMER_LEFT_1_SEQUENCE=CAATATAATACACGATGGCCGC
PRIMER_RIGHT_1_SEQUENCE=ACTCCTCATGGTGAACCTTGTC
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=194,22
PRIMER_LEFT_1_TM=61.392
PRIMER_RIGHT_1_TM=60.414
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=12.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=1.8056
PRIMER_LEFT_2_PENALTY=1.391547
PRIMER_RIGHT_2_PENALTY=0.414033
PRIMER_LEFT_2_SEQUENCE=GCAATATAATACACGATGGCCG
PRIMER_RIGHT_2_SEQUENCE=ACTCCTCATGGTGAACCTTGTC
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=194,22
PRIMER_LEFT_2_TM=61.392
PRIMER_RIGHT_2_TM=60.414
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=12.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=1.8368
PRIMER_LEFT_3_PENALTY=1.083165
PRIMER_RIGHT_3_PENALTY=0.753664
PRIMER_LEFT_3_SEQUENCE=CGGGGTACCATTGATAAGGAT
PRIMER_RIGHT_3_SEQUENCE=AGGTTGGAGAAGAGAGAGCCAT
PRIMER_LEFT_3=2,21
PRIMER_RIGHT_3=121,22
PRIMER_LEFT_3_TM=59.917
PRIMER_RIGHT_3_TM=60.754
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=2.0851
PRIMER_LEFT_4_PENALTY=1.083165
PRIMER_RIGHT_4_PENALTY=1.001941
PRIMER_LEFT_4_SEQUENCE=CGGGGTACCATTGATAAGGAT
PRIMER_RIGHT_4_SEQUENCE=AGCCAAGGTTGGAGAAGAGAG
PRIMER_LEFT_4=2,21
PRIMER_RIGHT_4=126,21
PRIMER_LEFT_4_TM=59.917
PRIMER_RIGHT_4_TM=60.002
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_02704 Ps_contig_mira-and-tgicl-ass_franssen_23971
SEQUENCE=TGCAAGAGGAAGATGAGCTGCCCCCTTAGTTAAAAGTTCTTTTGCAAATTGCTAATGTGGGAAGGCAGGACGCTAAGAAGCATCTTTGAAGAGCACGTTTCTAATTTAATGTCTTCGAACATTGTTCAGACTTTGGGAATGATGCTCGACACAGTTGTGTTCTAGAGTTAGGATCGTCCACTGTATTCTTCTTCTTCTTCTTCATGATGTCCAGCTAATTATTTCATTTACACTTCTTTGACCTTGTTTGACAATAGTTATTTTGTTTTTTAATAACAAAGTTTNTTTGCTATTTCTAGTTTGGTTCGTTCCGTTACCTAACTAAAGTTTCGGTTTCCGGATAG
TARGET=186,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0613
PRIMER_LEFT_PENALTY=0.026278
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=GCTGCCCCCTTAGTTAAAAGTT
PRIMER_RIGHT_SEQUENCE=AACGGAACGAACCAAACTAGAA
PRIMER_LEFT=16,22
PRIMER_RIGHT=314,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=0.0613
PRIMER_LEFT_1_PENALTY=0.026278
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=GCTGCCCCCTTAGTTAAAAGTT
PRIMER_RIGHT_1_SEQUENCE=ACGGAACGAACCAAACTAGAAA
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=313,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.0643
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.035021
PRIMER_LEFT_2_SEQUENCE=TGTTCAGACTTTGGGAATGATG
PRIMER_RIGHT_2_SEQUENCE=ACGGAACGAACCAAACTAGAAA
PRIMER_LEFT_2=122,22
PRIMER_RIGHT_2=313,22
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=60.035
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=192
PRIMER_PAIR_PENALTY_3=0.0643
PRIMER_LEFT_3_PENALTY=0.029253
PRIMER_RIGHT_3_PENALTY=0.035021
PRIMER_LEFT_3_SEQUENCE=TGTTCAGACTTTGGGAATGATG
PRIMER_RIGHT_3_SEQUENCE=AACGGAACGAACCAAACTAGAA
PRIMER_LEFT_3=122,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=59.971
PRIMER_RIGHT_3_TM=60.035
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.1018
PRIMER_LEFT_4_PENALTY=0.029253
PRIMER_RIGHT_4_PENALTY=0.072552
PRIMER_LEFT_4_SEQUENCE=TGTTCAGACTTTGGGAATGATG
PRIMER_RIGHT_4_SEQUENCE=AGTTAGGTAACGGAACGAACCA
PRIMER_LEFT_4=122,22
PRIMER_RIGHT_4=322,22
PRIMER_LEFT_4_TM=59.971
PRIMER_RIGHT_4_TM=59.927
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=201
=
PRIMER_SEQUENCE_ID=SSR_02705 Ps_contig_mira-and-tgicl-ass_franssen_23979
SEQUENCE=TAGATCTCAACANCTANAATTAAACTTAAATAATCATAAACGATACCCATCTAAACACTACTCCCTCCCAGTTTCCACCGCCACCGCCTCCTCCACGAGAATAACCGCCTCCTCCCACCACTTCCTCCATACCCACGATCCCGGCTTCCGCCATAACCACCACCACCATATCCTCCAACACCAGTTTACAAGTATAAKTCACCACCTCCACCATCACCAACATATGTGTACAAGTCTCCTCCTCCTCCAACACCAGTTTACAAGTATAAGTCACCACCTCCACCATCACCAACATATGTGTACAAGTCTCCTCCTCCTCCAACACCRGTATACAAACCTCCATACTACTACAATTCACCTCCTCCTCCAACACCAGTTTACAAATACAAATCACCACCTCCTCCATCACCAACTTACGTGTACAAGTCTCCTCCTCCACCAACACCAGTATACAAACCCCCATACTACTATAACTCACCTCCTCCTCCAACACCAGTTTACAAGTATAATTCACCACCTCCACCATCACCAACTTACGTGTACAAGTCTCCTCCTCCTCCCGCACCAGTTTATAAGTACAACTCACCACCTCCTCCAACACCAGTATACAAATACWMTCTCTCACCTTGCCATCTCAAGCAAAACAATTCCTCTATTCA
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02706 Ps_contig_mira-and-tgicl-ass_franssen_23979
SEQUENCE=TAGATCTCAACANCTANAATTAAACTTAAATAATCATAAACGATACCCATCTAAACACTACTCCCTCCCAGTTTCCACCGCCACCGCCTCCTCCACGAGAATAACCGCCTCCTCCCACCACTTCCTCCATACCCACGATCCCGGCTTCCGCCATAACCACCACCACCATATCCTCCAACACCAGTTTACAAGTATAAKTCACCACCTCCACCATCACCAACATATGTGTACAAGTCTCCTCCTCCTCCAACACCAGTTTACAAGTATAAGTCACCACCTCCACCATCACCAACATATGTGTACAAGTCTCCTCCTCCTCCAACACCRGTATACAAACCTCCATACTACTACAATTCACCTCCTCCTCCAACACCAGTTTACAAATACAAATCACCACCTCCTCCATCACCAACTTACGTGTACAAGTCTCCTCCTCCACCAACACCAGTATACAAACCCCCATACTACTATAACTCACCTCCTCCTCCAACACCAGTTTACAAGTATAATTCACCACCTCCACCATCACCAACTTACGTGTACAAGTCTCCTCCTCCTCCCGCACCAGTTTATAAGTACAACTCACCACCTCCTCCAACACCAGTATACAAATACWMTCTCTCACCTTGCCATCTCAAGCAAAACAATTCCTCTATTCA
TARGET=236,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02707 Ps_contig_mira-and-tgicl-ass_franssen_23979
SEQUENCE=TAGATCTCAACANCTANAATTAAACTTAAATAATCATAAACGATACCCATCTAAACACTACTCCCTCCCAGTTTCCACCGCCACCGCCTCCTCCACGAGAATAACCGCCTCCTCCCACCACTTCCTCCATACCCACGATCCCGGCTTCCGCCATAACCACCACCACCATATCCTCCAACACCAGTTTACAAGTATAAKTCACCACCTCCACCATCACCAACATATGTGTACAAGTCTCCTCCTCCTCCAACACCAGTTTACAAGTATAAGTCACCACCTCCACCATCACCAACATATGTGTACAAGTCTCCTCCTCCTCCAACACCRGTATACAAACCTCCATACTACTACAATTCACCTCCTCCTCCAACACCAGTTTACAAATACAAATCACCACCTCCTCCATCACCAACTTACGTGTACAAGTCTCCTCCTCCACCAACACCAGTATACAAACCCCCATACTACTATAACTCACCTCCTCCTCCAACACCAGTTTACAAGTATAATTCACCACCTCCACCATCACCAACTTACGTGTACAAGTCTCCTCCTCCTCCCGCACCAGTTTATAAGTACAACTCACCACCTCCTCCAACACCAGTATACAAATACWMTCTCTCACCTTGCCATCTCAAGCAAAACAATTCCTCTATTCA
TARGET=308,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02708 Ps_contig_mira-and-tgicl-ass_franssen_23979
SEQUENCE=TAGATCTCAACANCTANAATTAAACTTAAATAATCATAAACGATACCCATCTAAACACTACTCCCTCCCAGTTTCCACCGCCACCGCCTCCTCCACGAGAATAACCGCCTCCTCCCACCACTTCCTCCATACCCACGATCCCGGCTTCCGCCATAACCACCACCACCATATCCTCCAACACCAGTTTACAAGTATAAKTCACCACCTCCACCATCACCAACATATGTGTACAAGTCTCCTCCTCCTCCAACACCAGTTTACAAGTATAAGTCACCACCTCCACCATCACCAACATATGTGTACAAGTCTCCTCCTCCTCCAACACCRGTATACAAACCTCCATACTACTACAATTCACCTCCTCCTCCAACACCAGTTTACAAATACAAATCACCACCTCCTCCATCACCAACTTACGTGTACAAGTCTCCTCCTCCACCAACACCAGTATACAAACCCCCATACTACTATAACTCACCTCCTCCTCCAACACCAGTTTACAAGTATAATTCACCACCTCCACCATCACCAACTTACGTGTACAAGTCTCCTCCTCCTCCCGCACCAGTTTATAAGTACAACTCACCACCTCCTCCAACACCAGTATACAAATACWMTCTCTCACCTTGCCATCTCAAGCAAAACAATTCCTCTATTCA
TARGET=548,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02709 Ps_contig_mira-and-tgicl-ass_franssen_23988
SEQUENCE=TGGCTATGGTCATGGACATGGAGGCTACGGTCATGGAGGCTACGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACAATGGTGGCGGAGGAGGATACAACCATGGAGGCGGTGGTTACAATGGTGGTGGAGGAGGCTACAACCATGGAGGTGGTGGTTATCGTGGTGGAGGTGGACATGGTGGTAGTTTCAACAATGGTAACTGATTATATCATCATGCATGCATGTGTTAAGTGTAATAAATAATGTTGGGTTTTCAACAAAAGAGTAAAGATAAATCACTATGTATATGTGAACATGAGTTTGATTTCGAGTAAAAGTGGTGAATGTGTTATCATGTGTAATATATCAAATATCATCGTTGTTAGTACTTTGCCTTCCTTTTATATAATCCATTCCGCTGTATTTGTAGTAAAGTAAAA
TARGET=95,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0930
PRIMER_LEFT_PENALTY=1.355039
PRIMER_RIGHT_PENALTY=0.737989
PRIMER_LEFT_SEQUENCE=GCTATGGTCATGGACATGGAG
PRIMER_RIGHT_SEQUENCE=ACCATTGTTGAAACTACCACCA
PRIMER_LEFT=2,21
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=60.355
PRIMER_RIGHT_TM=59.262
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=2.1959
PRIMER_LEFT_1_PENALTY=2.042732
PRIMER_RIGHT_1_PENALTY=0.153161
PRIMER_LEFT_1_SEQUENCE=GTCATGGAGGCTACGGTCAT
PRIMER_RIGHT_1_SEQUENCE=AAACTACCACCATGTCCACCTC
PRIMER_LEFT_1=29,20
PRIMER_RIGHT_1=245,22
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=60.153
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=2.1959
PRIMER_LEFT_2_PENALTY=2.042732
PRIMER_RIGHT_2_PENALTY=0.153161
PRIMER_LEFT_2_SEQUENCE=GTCATGGAGGCTACGGTCAT
PRIMER_RIGHT_2_SEQUENCE=GAAACTACCACCATGTCCACCT
PRIMER_LEFT_2=29,20
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=59.957
PRIMER_RIGHT_2_TM=60.153
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=2.4300
PRIMER_LEFT_3_PENALTY=2.276885
PRIMER_RIGHT_3_PENALTY=0.153161
PRIMER_LEFT_3_SEQUENCE=GCTACGGTCATGGAGGCTAC
PRIMER_RIGHT_3_SEQUENCE=AAACTACCACCATGTCCACCTC
PRIMER_LEFT_3=23,20
PRIMER_RIGHT_3=245,22
PRIMER_LEFT_3_TM=59.723
PRIMER_RIGHT_3_TM=60.153
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=2.4300
PRIMER_LEFT_4_PENALTY=2.276885
PRIMER_RIGHT_4_PENALTY=0.153161
PRIMER_LEFT_4_SEQUENCE=GCTACGGTCATGGAGGCTAC
PRIMER_RIGHT_4_SEQUENCE=GAAACTACCACCATGTCCACCT
PRIMER_LEFT_4=23,20
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=59.723
PRIMER_RIGHT_4_TM=60.153
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_02710 Ps_contig_mira-and-tgicl-ass_franssen_23988
SEQUENCE=TGGCTATGGTCATGGACATGGAGGCTACGGTCATGGAGGCTACGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACAATGGTGGCGGAGGAGGATACAACCATGGAGGCGGTGGTTACAATGGTGGTGGAGGAGGCTACAACCATGGAGGTGGTGGTTATCGTGGTGGAGGTGGACATGGTGGTAGTTTCAACAATGGTAACTGATTATATCATCATGCATGCATGTGTTAAGTGTAATAAATAATGTTGGGTTTTCAACAAAAGAGTAAAGATAAATCACTATGTATATGTGAACATGAGTTTGATTTCGAGTAAAAGTGGTGAATGTGTTATCATGTGTAATATATCAAATATCATCGTTGTTAGTACTTTGCCTTCCTTTTATATAATCCATTCCGCTGTATTTGTAGTAAAGTAAAA
TARGET=119,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0930
PRIMER_LEFT_PENALTY=1.355039
PRIMER_RIGHT_PENALTY=0.737989
PRIMER_LEFT_SEQUENCE=GCTATGGTCATGGACATGGAG
PRIMER_RIGHT_SEQUENCE=ACCATTGTTGAAACTACCACCA
PRIMER_LEFT=2,21
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=60.355
PRIMER_RIGHT_TM=59.262
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=2.1959
PRIMER_LEFT_1_PENALTY=2.042732
PRIMER_RIGHT_1_PENALTY=0.153161
PRIMER_LEFT_1_SEQUENCE=GTCATGGAGGCTACGGTCAT
PRIMER_RIGHT_1_SEQUENCE=AAACTACCACCATGTCCACCTC
PRIMER_LEFT_1=29,20
PRIMER_RIGHT_1=245,22
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=60.153
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=2.1959
PRIMER_LEFT_2_PENALTY=2.042732
PRIMER_RIGHT_2_PENALTY=0.153161
PRIMER_LEFT_2_SEQUENCE=GTCATGGAGGCTACGGTCAT
PRIMER_RIGHT_2_SEQUENCE=GAAACTACCACCATGTCCACCT
PRIMER_LEFT_2=29,20
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=59.957
PRIMER_RIGHT_2_TM=60.153
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=2.4300
PRIMER_LEFT_3_PENALTY=2.276885
PRIMER_RIGHT_3_PENALTY=0.153161
PRIMER_LEFT_3_SEQUENCE=GCTACGGTCATGGAGGCTAC
PRIMER_RIGHT_3_SEQUENCE=AAACTACCACCATGTCCACCTC
PRIMER_LEFT_3=23,20
PRIMER_RIGHT_3=245,22
PRIMER_LEFT_3_TM=59.723
PRIMER_RIGHT_3_TM=60.153
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=2.4300
PRIMER_LEFT_4_PENALTY=2.276885
PRIMER_RIGHT_4_PENALTY=0.153161
PRIMER_LEFT_4_SEQUENCE=GCTACGGTCATGGAGGCTAC
PRIMER_RIGHT_4_SEQUENCE=GAAACTACCACCATGTCCACCT
PRIMER_LEFT_4=23,20
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=59.723
PRIMER_RIGHT_4_TM=60.153
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_02711 Ps_contig_mira-and-tgicl-ass_franssen_23988
SEQUENCE=TGGCTATGGTCATGGACATGGAGGCTACGGTCATGGAGGCTACGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACAATGGTGGCGGAGGAGGATACAACCATGGAGGCGGTGGTTACAATGGTGGTGGAGGAGGCTACAACCATGGAGGTGGTGGTTATCGTGGTGGAGGTGGACATGGTGGTAGTTTCAACAATGGTAACTGATTATATCATCATGCATGCATGTGTTAAGTGTAATAAATAATGTTGGGTTTTCAACAAAAGAGTAAAGATAAATCACTATGTATATGTGAACATGAGTTTGATTTCGAGTAAAAGTGGTGAATGTGTTATCATGTGTAATATATCAAATATCATCGTTGTTAGTACTTTGCCTTCCTTTTATATAATCCATTCCGCTGTATTTGTAGTAAAGTAAAA
TARGET=272,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4375
PRIMER_LEFT_PENALTY=0.153161
PRIMER_RIGHT_PENALTY=1.284310
PRIMER_LEFT_SEQUENCE=AGGTGGACATGGTGGTAGTTTC
PRIMER_RIGHT_SEQUENCE=GGAAGGCAAAGTACTAACAACGA
PRIMER_LEFT=225,22
PRIMER_RIGHT=436,23
PRIMER_LEFT_TM=60.153
PRIMER_RIGHT_TM=59.716
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=1.4375
PRIMER_LEFT_1_PENALTY=0.153161
PRIMER_RIGHT_1_PENALTY=1.284310
PRIMER_LEFT_1_SEQUENCE=GAGGTGGACATGGTGGTAGTTT
PRIMER_RIGHT_1_SEQUENCE=GGAAGGCAAAGTACTAACAACGA
PRIMER_LEFT_1=224,22
PRIMER_RIGHT_1=436,23
PRIMER_LEFT_1_TM=60.153
PRIMER_RIGHT_1_TM=59.716
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=1.4999
PRIMER_LEFT_2_PENALTY=0.153161
PRIMER_RIGHT_2_PENALTY=1.346747
PRIMER_LEFT_2_SEQUENCE=GAGGTGGACATGGTGGTAGTTT
PRIMER_RIGHT_2_SEQUENCE=ACTACAAATACAGCGGAATGGA
PRIMER_LEFT_2=224,22
PRIMER_RIGHT_2=468,22
PRIMER_LEFT_2_TM=60.153
PRIMER_RIGHT_2_TM=58.653
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=1.4999
PRIMER_LEFT_3_PENALTY=0.153161
PRIMER_RIGHT_3_PENALTY=1.346747
PRIMER_LEFT_3_SEQUENCE=AGGTGGACATGGTGGTAGTTTC
PRIMER_RIGHT_3_SEQUENCE=ACTACAAATACAGCGGAATGGA
PRIMER_LEFT_3=225,22
PRIMER_RIGHT_3=468,22
PRIMER_LEFT_3_TM=60.153
PRIMER_RIGHT_3_TM=58.653
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=1.5271
PRIMER_LEFT_4_PENALTY=0.242742
PRIMER_RIGHT_4_PENALTY=1.284310
PRIMER_LEFT_4_SEQUENCE=GGACATGGTGGTAGTTTCAACA
PRIMER_RIGHT_4_SEQUENCE=GGAAGGCAAAGTACTAACAACGA
PRIMER_LEFT_4=229,22
PRIMER_RIGHT_4=436,23
PRIMER_LEFT_4_TM=59.757
PRIMER_RIGHT_4_TM=59.716
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_02712 Ps_contig_mira-and-tgicl-ass_franssen_23998
SEQUENCE=ATTTGAGTATAATGGATTCCAAAATAGCAATTCTCATTCTTGGCCTAATGGCCATGGTTCTTGTTTATTTCCTCAGAGGTGTCAGCTAGGGACTTAACTGAGACTTCAACTAATACCAAAGATGACGTTGTTGAAAAGTCAGATGAATTAAATGATGCCAAATATTACGGTGGAGGTTACAACCATGGTGGCGGCGGAGGTTACAACGGTGGTGGAGGAGGCTACAACCATGGTGGTGGTGGCTACAATGGTGGTGGAGGAGGATACAACCATGGAGGTGGTGGTTACAATGGTGGCGGAGGAGGATACAACCATGGAGGCGGTGGTTACAACGGTGGCGGTGGAGGATACAACCATGGTGGCGGAGGAGGATACAACCATGGAGGCGGTGGTTACAATGGTGGTGGAGGAGGCTACAACCATGGAGGTGGTGGTTATCGTGGTGGAGGTGGACATGGTGGTAGTTTCAAACAATGGTAACTGATTATATCAACATGCATGCTATTGTAGTTAAAAG
TARGET=231,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0370
PRIMER_LEFT_PENALTY=0.061961
PRIMER_RIGHT_PENALTY=0.975014
PRIMER_LEFT_SEQUENCE=CAGAGGTGTCAGCTAGGGACTT
PRIMER_RIGHT_SEQUENCE=ACCTCCATGGTTGTATCCTCCT
PRIMER_LEFT=73,22
PRIMER_RIGHT=278,22
PRIMER_LEFT_TM=59.938
PRIMER_RIGHT_TM=60.975
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=1.1285
PRIMER_LEFT_1_PENALTY=0.061961
PRIMER_RIGHT_1_PENALTY=1.066501
PRIMER_LEFT_1_SEQUENCE=CAGAGGTGTCAGCTAGGGACTT
PRIMER_RIGHT_1_SEQUENCE=ATGGTTGTATCCTCCTCCACC
PRIMER_LEFT_1=73,22
PRIMER_RIGHT_1=272,21
PRIMER_LEFT_1_TM=59.938
PRIMER_RIGHT_1_TM=60.067
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=1.1285
PRIMER_LEFT_2_PENALTY=0.061961
PRIMER_RIGHT_2_PENALTY=1.066501
PRIMER_LEFT_2_SEQUENCE=CAGAGGTGTCAGCTAGGGACTT
PRIMER_RIGHT_2_SEQUENCE=GTATCCTCCTCCACCACCATT
PRIMER_LEFT_2=73,22
PRIMER_RIGHT_2=266,21
PRIMER_LEFT_2_TM=59.938
PRIMER_RIGHT_2_TM=60.067
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=1.1285
PRIMER_LEFT_3_PENALTY=0.061961
PRIMER_RIGHT_3_PENALTY=1.066501
PRIMER_LEFT_3_SEQUENCE=CAGAGGTGTCAGCTAGGGACTT
PRIMER_RIGHT_3_SEQUENCE=ACCTCCATGGTTGTATCCTCC
PRIMER_LEFT_3=73,22
PRIMER_RIGHT_3=278,21
PRIMER_LEFT_3_TM=59.938
PRIMER_RIGHT_3_TM=60.067
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=1.1655
PRIMER_LEFT_4_PENALTY=0.061961
PRIMER_RIGHT_4_PENALTY=1.103525
PRIMER_LEFT_4_SEQUENCE=CAGAGGTGTCAGCTAGGGACTT
PRIMER_RIGHT_4_SEQUENCE=CACCATGGTTGTATCCTCCAC
PRIMER_LEFT_4=73,22
PRIMER_RIGHT_4=360,21
PRIMER_LEFT_4_TM=59.938
PRIMER_RIGHT_4_TM=60.104
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_02713 Ps_contig_mira-and-tgicl-ass_franssen_24006
SEQUENCE=TCAAGAGTGTGGAGAAGRAGGACCCAACTGGTAGCCAAAGGTCACCAAAAGCAGCAGCCAAAGAAAGAAAGTGATTTCACTCCATGGTTGCTTCTTGCACAAAGTTTG
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02714 Ps_contig_mira-and-tgicl-ass_franssen_24023
SEQUENCE=TAGTTCACGGTTCCTATATCTCCGGTCTTGTAAACCTTTTTAATGGCCACTGCCTCTCCGGTTTCCAAGCATTTCGCCTGGAAAACAATTCCAAATGATCCAGTTCCTACAACACGTTCTGCCATGTAACTAATAGTCTGTTTGGGTTCACCGTTTTTGCCTCCAATTGTTGTAGATATTATTTGACCAGTGAGAGAACCATTCCCGTTGATAACAGAAGTAGACATCTCCTTATCCTCAGCCATGAGGAAGAAAGGAGGGAGAGAGAGAGAGAGGAGAGAGAAGTAAGAGGGAGGNGAAGAGGAGGGAGAGTGGTAGGAAGAAGAAAG
TARGET=261,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0672
PRIMER_LEFT_PENALTY=0.035219
PRIMER_RIGHT_PENALTY=0.031944
PRIMER_LEFT_SEQUENCE=ACAACACGTTCTGCCATGTAAC
PRIMER_RIGHT_SEQUENCE=CTACCACTCTCCCTCCTCTTCN
PRIMER_LEFT=108,22
PRIMER_RIGHT=317,22
PRIMER_LEFT_TM=59.965
PRIMER_RIGHT_TM=60.032
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=57.143
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=7.6500
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.1276
PRIMER_LEFT_1_PENALTY=0.095684
PRIMER_RIGHT_1_PENALTY=0.031944
PRIMER_LEFT_1_SEQUENCE=CAACACGTTCTGCCATGTAACT
PRIMER_RIGHT_1_SEQUENCE=CTACCACTCTCCCTCCTCTTCN
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=317,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.032
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=57.143
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.6500
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.1718
PRIMER_LEFT_2_PENALTY=0.035219
PRIMER_RIGHT_2_PENALTY=0.136583
PRIMER_LEFT_2_SEQUENCE=ACAACACGTTCTGCCATGTAAC
PRIMER_RIGHT_2_SEQUENCE=TCCTCTTCNCCTCCCTCTTACT
PRIMER_LEFT_2=108,22
PRIMER_RIGHT_2=304,22
PRIMER_LEFT_2_TM=59.965
PRIMER_RIGHT_2_TM=60.137
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=197
PRIMER_PAIR_PENALTY_3=0.1718
PRIMER_LEFT_3_PENALTY=0.035219
PRIMER_RIGHT_3_PENALTY=0.136583
PRIMER_LEFT_3_SEQUENCE=ACAACACGTTCTGCCATGTAAC
PRIMER_RIGHT_3_SEQUENCE=CTCCTCTTCNCCTCCCTCTTAC
PRIMER_LEFT_3=108,22
PRIMER_RIGHT_3=305,22
PRIMER_LEFT_3_TM=59.965
PRIMER_RIGHT_3_TM=60.137
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=57.143
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=0.1903
PRIMER_LEFT_4_PENALTY=0.158317
PRIMER_RIGHT_4_PENALTY=0.031944
PRIMER_LEFT_4_SEQUENCE=CAGTTCCTACAACACGTTCTGC
PRIMER_RIGHT_4_SEQUENCE=CTACCACTCTCCCTCCTCTTCN
PRIMER_LEFT_4=100,22
PRIMER_RIGHT_4=317,22
PRIMER_LEFT_4_TM=59.842
PRIMER_RIGHT_4_TM=60.032
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6500
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_02715 Ps_contig_mira-and-tgicl-ass_franssen_24028
SEQUENCE=GACACACATCATCGAACCCACGCGTCACACCGATAAACCACTCACCACAACCATTTCTCTTCCCTAATCAAACCACTCTCACGCTCCATCTTCAACCAACACCATGATGTGGGAATGGGAACAAGACGAACACCACCACCACCGCCACAACAACGCCTCTCCAAAACCCGNTCAAATCCCTGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGACCGTAGCCT
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8246
PRIMER_LEFT_PENALTY=0.344527
PRIMER_RIGHT_PENALTY=1.480087
PRIMER_LEFT_SEQUENCE=CCACAACCATTTCTCTTCCCTA
PRIMER_RIGHT_SEQUENCE=AGGTTACGGTCAGGGATTTGAN
PRIMER_LEFT=44,22
PRIMER_RIGHT=191,22
PRIMER_LEFT_TM=60.345
PRIMER_RIGHT_TM=61.480
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.50
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=6.9750
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.75
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=1.8597
PRIMER_LEFT_1_PENALTY=0.344527
PRIMER_RIGHT_1_PENALTY=1.515201
PRIMER_LEFT_1_SEQUENCE=CCACAACCATTTCTCTTCCCTA
PRIMER_RIGHT_1_SEQUENCE=GTTACGGTCAGGGATTTGANC
PRIMER_LEFT_1=44,22
PRIMER_RIGHT_1=189,21
PRIMER_LEFT_1_TM=60.345
PRIMER_RIGHT_1_TM=59.485
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.75
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=7.3500
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=1.9654
PRIMER_LEFT_2_PENALTY=0.344527
PRIMER_RIGHT_2_PENALTY=1.620879
PRIMER_LEFT_2_SEQUENCE=CCACAACCATTTCTCTTCCCTA
PRIMER_RIGHT_2_SEQUENCE=GGTTACGGTCAGGGATTTGAN
PRIMER_LEFT_2=44,22
PRIMER_RIGHT_2=190,21
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=60.621
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.50
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=6.9750
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=2.0285
PRIMER_LEFT_3_PENALTY=0.548390
PRIMER_RIGHT_3_PENALTY=1.480087
PRIMER_LEFT_3_SEQUENCE=AAACCACTCACCACAACCATTT
PRIMER_RIGHT_3_SEQUENCE=AGGTTACGGTCAGGGATTTGAN
PRIMER_LEFT_3=34,22
PRIMER_RIGHT_3=191,22
PRIMER_LEFT_3_TM=60.548
PRIMER_RIGHT_3_TM=61.480
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.50
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.9750
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=2.0636
PRIMER_LEFT_4_PENALTY=0.548390
PRIMER_RIGHT_4_PENALTY=1.515201
PRIMER_LEFT_4_SEQUENCE=AAACCACTCACCACAACCATTT
PRIMER_RIGHT_4_SEQUENCE=GTTACGGTCAGGGATTTGANC
PRIMER_LEFT_4=34,22
PRIMER_RIGHT_4=189,21
PRIMER_LEFT_4_TM=60.548
PRIMER_RIGHT_4_TM=59.485
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.75
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.3500
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=156
=
PRIMER_SEQUENCE_ID=SSR_02716 Ps_contig_mira-and-tgicl-ass_franssen_24105
SEQUENCE=TTCGGGTAGAGGAAGCAAAGGAAGAGAACTTAGAAGCACCTAAAGAAACTGAGGAATCGGTTGAAGAAGTGAAGGAAGAGGCGGAAGCARKTAGTGGAGGAGCCAATAGCAACTACTGAAAATGAGTCAGAACACCACCACCACCAGCAACTGCAGAG
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02717 Ps_contig_mira-and-tgicl-ass_franssen_24111
SEQUENCE=AACGCAGAGTACNGTTTTTTTGTTGTTTTTTACAAAATATTTTTTRATTTGTTCTGCTCGGATCCTCAAAAACGCRTGTTCTGTTTCTTTGGAGTTCATAGARRAAAAATTACYTTGACAATTTCTTTTGGATTTTAYAAAACGTGTTACTGTTAATTAATTTTGTGGTTATTATACATGAATGTTGGTTTCTGTGAGATAACTTGTTGGATCCTATTTCCAGATTTTGTGGGATGATTAATCTATGGATTTAAAGTTGTTTATTTGGATAATGCAGTGTGTTTCTGAGCGCTTCCTTTGATTCATTGTGTAACATATTAATCTGCTCTTTCTATTAATTATTTTCCAGCTTTTTAAGTGCTTATTTGATAAGTTGTTTTTAATTTTGAATTGGTTTTGCAGGTGAAGTGATGTTGATGTTGATTGATTGAAAAAGGGGCGCTGTTATTCAACAAGTTTTGGAAAAGTTCTGAAGAGTTGCTTTTGAATTGGGAACTTCCAAAAGAGCTTTTGGAAAAGTCTGTGAAATTTGCTTCAAACTGCTGTGTTTACAGCTTATTCCAATTTTTTAAATAAAATTATTTCTGATGTAACAGAGACCTTTTGGATTATGAACTTAGGGATTGCTTGTAACATGTACAAACTTAATTTGGAATTTCTCTTTTTGGCCAGTTATGTAATTGTTTTGGATTATTAAAGGAATTTTTGGATTAGTTAGGATATATTAAACTGTAAGAAAAAATTGGGTTAGACGTGTAACCCTTTGGAAWCMTTGNAAATTACATAATTTTAATTTAATTTTTTNTTATTTATGTGTA
TARGET=420,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02718 Ps_contig_mira-and-tgicl-ass_franssen_24133
SEQUENCE=GCAAAATACAAAGCATACAATTTTGGCAAATTTATTTATTTRTKCTTAATAGAACCCATGCTGATTTTGGCAATCAAGACATTGAGGGTAGAGAAATTCATCACTTCAAGATTCTGGATTAGCTATTAAAAAATATTCAGCATCTGTTTTGGATTTTCTGTTGTTTTTCATGTCCTTATTCTCATCAAAGACATAATAAATTGAGCTCCCTTTATGAAATCGTCGTAACACATGTGTTGCAAAAATTAAAAAAATTCTTGTAATGAAACTTGTCCTCTAATTTAAATAAATGTGTTGTTCCTTWCTTTAWAAAAGTTTT
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02719 Ps_contig_mira-and-tgicl-ass_franssen_24138
SEQUENCE=TGCGGGAGGGTGGGAGGGTGGGACATGGTGGTAGTTCCAACAATGGTAACTGATTATGTCACCATGCATGCATGTACTATGTGTAATCATAGAAATAAATTACTTGGTTTGGGTTTACAAANAAAGAGTATATAGAATAA
TARGET=63,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02720 Ps_contig_mira-and-tgicl-ass_franssen_24191
SEQUENCE=GTCTTTGTTGGTTATTATTGGGTTGCAAGGATAGATCACTACTCTCCACTGTGCGTGCTGTTGTAARKTTTAAGAAGAAWTAAAGTTCGACCTAACCTACTACCTTACCCCTTCCTTTACGTTCGTTCGTTTTTAGGGTTTTAACGGTT
TARGET=120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02721 Ps_contig_mira-and-tgicl-ass_franssen_24234
SEQUENCE=GTAATAGAACAGTATTTATTGCCGTTATAGTGTTCAGTGTTAACTAAGTTGCCGTGCTACTTAATAGCATACTCCCAGTTTGATACTTTTTATTAAAAAGTTGCATTGTCAAATTATTTAATTTCTTGCATGTGTATATCTAAATATGCATGGTGTTGACTCCTCTTGAGCTAAATAAAACAAATGTAGTAGTGTCTTTGATTGTATTGTGACATCAATAATGTTGACATTGTTCAATGATTGTCATTTCATTTCATTATTTTGAGTTTGCCAGTGACTGTTGTGTTGGTTTTATGTACTTGAATTTGATGTCGGAATATGAAAAGCAAGCYCTATTTATCATCAAACAGCGATGAAAGCAATTTATT
TARGET=244,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02722 Ps_contig_mira-and-tgicl-ass_franssen_24249
SEQUENCE=GGTGGCGGCAGTTACCACGGTGGACATGGTGGTACTATTGGTAGGACATGGTGGACATGGTGGTGTTTCCAACAATGAGGTTGTTGAAACTTCAAATGACGTAAATGATGCCAAATATGGACACTACGGTCATGGTGGTGGTAGTTACCATGGTGGTGGTGGCGGTTACCACGGTGGACATGGAGGACATGGTGGACATGGTGGACACGGTGGTGCTTCCGACAATGGCAACTGATAATACCATGATGCATGATGCACATTCAAAGTAATAAGTTTCATGAATGAATAGTGTTTGGTGTAAAATAAAAGAGGTATCATCTTTGTATTTGTGGTGAAGAGTTTGATTCCAGTGAAAGTGATGAATGTGTTATCATTTGTAATATATCAAATATATATTATTGTTGTTTGTACTTTGATTCCTTTTAAGTTTGTTACTTTGCATATTACTGATTTGTYTAAGAAAATTGGTCGACTAATTGACG
TARGET=151,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02723 Ps_contig_mira-and-tgicl-ass_franssen_24309
SEQUENCE=TTACAACCATGGTGGCGGTGGAGGCGGATACAACCATGGAGGTGGTGGTTACAATGGTGGCGGAGGAGGATACAACCATGGTGGCGGAGGAGGATACAACCATGGAGGTGGTGGTTATAATGGTGGCGGAGGAGGATACAACCATGGAGGTGGTGGTTACAATGGTGGTGGAGGAGGATACAACCATGGTGGTGGTGGTTATGGTGGTGGAGGTGGACATGGTGGTAGTTCCAACAATGGTAACTGACTATGTCACCATGCATGCATGTACTATGTGTAATCATGAATAAATACTGTTGGGTTTCCAACAAAAGAGTATATAGATAAATCACTATGTTATCATGTGTAATATATCAAATATCATCCTTGTTGTTAAGAAAAGTT
TARGET=187,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4074
PRIMER_LEFT_PENALTY=0.164639
PRIMER_RIGHT_PENALTY=0.242742
PRIMER_LEFT_SEQUENCE=ATGGAGGTGGTGGTTATAATGG
PRIMER_RIGHT_SEQUENCE=TTGTTGGAACTACCACCATGTC
PRIMER_LEFT=101,22
PRIMER_RIGHT=236,22
PRIMER_LEFT_TM=59.835
PRIMER_RIGHT_TM=59.757
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=0.5951
PRIMER_LEFT_1_PENALTY=0.352395
PRIMER_RIGHT_1_PENALTY=0.242742
PRIMER_LEFT_1_SEQUENCE=TGGAGGTGGTGGTTATAATGGT
PRIMER_RIGHT_1_SEQUENCE=TTGTTGGAACTACCACCATGTC
PRIMER_LEFT_1=102,22
PRIMER_RIGHT_1=236,22
PRIMER_LEFT_1_TM=60.352
PRIMER_RIGHT_1_TM=59.757
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=1.1553
PRIMER_LEFT_2_PENALTY=0.912566
PRIMER_RIGHT_2_PENALTY=0.242742
PRIMER_LEFT_2_SEQUENCE=CATGGAGGTGGTGGTTATAATG
PRIMER_RIGHT_2_SEQUENCE=TTGTTGGAACTACCACCATGTC
PRIMER_LEFT_2=100,22
PRIMER_RIGHT_2=236,22
PRIMER_LEFT_2_TM=59.087
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=1.1974
PRIMER_LEFT_3_PENALTY=0.164639
PRIMER_RIGHT_3_PENALTY=1.032744
PRIMER_LEFT_3_SEQUENCE=ATGGAGGTGGTGGTTATAATGG
PRIMER_RIGHT_3_SEQUENCE=ATTGTTGGAACTACCACCATGTC
PRIMER_LEFT_3=101,22
PRIMER_RIGHT_3=237,23
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=60.033
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=137
PRIMER_PAIR_PENALTY_4=1.2298
PRIMER_LEFT_4_PENALTY=0.164639
PRIMER_RIGHT_4_PENALTY=1.065200
PRIMER_LEFT_4_SEQUENCE=ATGGAGGTGGTGGTTATAATGG
PRIMER_RIGHT_4_SEQUENCE=CATGCATGGTGACATAGTCAGTT
PRIMER_LEFT_4=101,22
PRIMER_RIGHT_4=263,23
PRIMER_LEFT_4_TM=59.835
PRIMER_RIGHT_4_TM=59.935
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_02724 Ps_contig_mira-and-tgicl-ass_franssen_24309
SEQUENCE=TTACAACCATGGTGGCGGTGGAGGCGGATACAACCATGGAGGTGGTGGTTACAATGGTGGCGGAGGAGGATACAACCATGGTGGCGGAGGAGGATACAACCATGGAGGTGGTGGTTATAATGGTGGCGGAGGAGGATACAACCATGGAGGTGGTGGTTACAATGGTGGTGGAGGAGGATACAACCATGGTGGTGGTGGTTATGGTGGTGGAGGTGGACATGGTGGTAGTTCCAACAATGGTAACTGACTATGTCACCATGCATGCATGTACTATGTGTAATCATGAATAAATACTGTTGGGTTTCCAACAAAAGAGTATATAGATAAATCACTATGTTATCATGTGTAATATATCAAATATCATCCTTGTTGTTAAGAAAAGTT
TARGET=257,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=5.0173
PRIMER_LEFT_PENALTY=0.164639
PRIMER_RIGHT_PENALTY=4.852676
PRIMER_LEFT_SEQUENCE=ATGGAGGTGGTGGTTATAATGG
PRIMER_RIGHT_SEQUENCE=ACTCTTTTGTTGGAAACCCA
PRIMER_LEFT=101,22
PRIMER_RIGHT=316,20
PRIMER_LEFT_TM=59.835
PRIMER_RIGHT_TM=57.147
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=5.2051
PRIMER_LEFT_1_PENALTY=0.352395
PRIMER_RIGHT_1_PENALTY=4.852676
PRIMER_LEFT_1_SEQUENCE=GGAGGTGGTGGTTATAATGGTG
PRIMER_RIGHT_1_SEQUENCE=ACTCTTTTGTTGGAAACCCA
PRIMER_LEFT_1=103,22
PRIMER_RIGHT_1=316,20
PRIMER_LEFT_1_TM=60.352
PRIMER_RIGHT_1_TM=57.147
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=5.2051
PRIMER_LEFT_2_PENALTY=0.352395
PRIMER_RIGHT_2_PENALTY=4.852676
PRIMER_LEFT_2_SEQUENCE=TGGAGGTGGTGGTTATAATGGT
PRIMER_RIGHT_2_SEQUENCE=ACTCTTTTGTTGGAAACCCA
PRIMER_LEFT_2=102,22
PRIMER_RIGHT_2=316,20
PRIMER_LEFT_2_TM=60.352
PRIMER_RIGHT_2_TM=57.147
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=5.2051
PRIMER_LEFT_3_PENALTY=0.352395
PRIMER_RIGHT_3_PENALTY=4.852676
PRIMER_LEFT_3_SEQUENCE=GAGGTGGTGGTTATAATGGTGG
PRIMER_RIGHT_3_SEQUENCE=ACTCTTTTGTTGGAAACCCA
PRIMER_LEFT_3=104,22
PRIMER_RIGHT_3=316,20
PRIMER_LEFT_3_TM=60.352
PRIMER_RIGHT_3_TM=57.147
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=5.5224
PRIMER_LEFT_4_PENALTY=0.669753
PRIMER_RIGHT_4_PENALTY=4.852676
PRIMER_LEFT_4_SEQUENCE=GAGGTGGACATGGTGGTAGTTC
PRIMER_RIGHT_4_SEQUENCE=ACTCTTTTGTTGGAAACCCA
PRIMER_LEFT_4=209,22
PRIMER_RIGHT_4=316,20
PRIMER_LEFT_4_TM=60.670
PRIMER_RIGHT_4_TM=57.147
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_02725 Ps_contig_mira-and-tgicl-ass_franssen_24316
SEQUENCE=CTTAAAGTTTTACTTGCAACAAAACTTAGAAGAATTCGGTTGGTAGAATAACAATAACACAAGTCAGTAAATCAAGAAAGTAACTAAACCATTTAGGGTTAGGTAAGTCTTCAGGGCGACTGCCCTAACAAAACAAGATGTGTACTGCAGTTGAAAACACTAGATTAACAATATCTCGGTATCTTCTGAACAGTCGCAGTTACCGACTTTTAAAATCAACTTCTGCTTCGAGGTGCATCCCTTAACCCATTTCCGTTAGCATGTGCTACGGCCTCACGTCCACGGTAAGGAGGGGGTGACCTGCTGTAGCTTCGGCGACGAGGTGAAGGGCTGCGACGACGAGGAGATGGGCTTCGCCGCCTAGGGGACCGCTCACGAGGACTGTAACTCCTTCGCCCATAACTCGGGCTCCTACGCAATCGAGGTGGACTGCGGCTCCGACGCCTTCCACCACCACCACCGMCGMSAWGAMYGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGTCCAATAGACCATAGCCTCCATGTCCATGAC
TARGET=448,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02726 Ps_contig_mira-and-tgicl-ass_franssen_24316
SEQUENCE=CTTAAAGTTTTACTTGCAACAAAACTTAGAAGAATTCGGTTGGTAGAATAACAATAACACAAGTCAGTAAATCAAGAAAGTAACTAAACCATTTAGGGTTAGGTAAGTCTTCAGGGCGACTGCCCTAACAAAACAAGATGTGTACTGCAGTTGAAAACACTAGATTAACAATATCTCGGTATCTTCTGAACAGTCGCAGTTACCGACTTTTAAAATCAACTTCTGCTTCGAGGTGCATCCCTTAACCCATTTCCGTTAGCATGTGCTACGGCCTCACGTCCACGGTAAGGAGGGGGTGACCTGCTGTAGCTTCGGCGACGAGGTGAAGGGCTGCGACGACGAGGAGATGGGCTTCGCCGCCTAGGGGACCGCTCACGAGGACTGTAACTCCTTCGCCCATAACTCGGGCTCCTACGCAATCGAGGTGGACTGCGGCTCCGACGCCTTCCACCACCACCACCGMCGMSAWGAMYGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGTCCAATAGACCATAGCCTCCATGTCCATGAC
TARGET=477,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02727 Ps_contig_mira-and-tgicl-ass_franssen_24316
SEQUENCE=CTTAAAGTTTTACTTGCAACAAAACTTAGAAGAATTCGGTTGGTAGAATAACAATAACACAAGTCAGTAAATCAAGAAAGTAACTAAACCATTTAGGGTTAGGTAAGTCTTCAGGGCGACTGCCCTAACAAAACAAGATGTGTACTGCAGTTGAAAACACTAGATTAACAATATCTCGGTATCTTCTGAACAGTCGCAGTTACCGACTTTTAAAATCAACTTCTGCTTCGAGGTGCATCCCTTAACCCATTTCCGTTAGCATGTGCTACGGCCTCACGTCCACGGTAAGGAGGGGGTGACCTGCTGTAGCTTCGGCGACGAGGTGAAGGGCTGCGACGACGAGGAGATGGGCTTCGCCGCCTAGGGGACCGCTCACGAGGACTGTAACTCCTTCGCCCATAACTCGGGCTCCTACGCAATCGAGGTGGACTGCGGCTCCGACGCCTTCCACCACCACCACCGMCGMSAWGAMYGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGTCCAATAGACCATAGCCTCCATGTCCATGAC
TARGET=501,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02728 Ps_contig_mira-and-tgicl-ass_franssen_24328
SEQUENCE=TTTCTTGTACAACCTTCTCTCCCTTACACTGTGGGCAGCGATCTTTGTCATTGATAGTCTCACCAGTACCCTTACATTCATTGCAGGGATGCTGCATTTGCTGAATCATTGATGGACCGAGATGCCTAATAGAAACCTTCATACCAGTTCCTTGACAACCAGCACATTTCATGGAAGCTCCAGATTTAGAACCTTTGCCACTGCACTTTGAGCACAAGACATTCCTCGAGAGAGAAAGCTTCTTAGATGTTCCAAGATAAAGATCCTCCAGAGAGACCTTGAGAGGGTGAACAACATCTTCTCCACGTCTCTGCCTCCGTCCACGGCTACTACCACCGGCACCACCACCAAAGAAAGACGAGAAGATGTCAAATGGGTCATGGCCACCGCCACCACCACCACCCATTCCTTACCTTAAGAGACGCATCCTCACCATATGTAT
TARGET=390,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0110
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.010294
PRIMER_LEFT_SEQUENCE=CCAGATTTAGAACCTTTGCCAC
PRIMER_RIGHT_SEQUENCE=ATATGGTGAGGATGCGTCTCTT
PRIMER_LEFT=179,22
PRIMER_RIGHT=437,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=0.0476
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.010294
PRIMER_LEFT_1_SEQUENCE=CTTGACAACCAGCACATTTCAT
PRIMER_RIGHT_1_SEQUENCE=ATATGGTGAGGATGCGTCTCTT
PRIMER_LEFT_1=150,22
PRIMER_RIGHT_1=437,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.990
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.0833
PRIMER_LEFT_2_PENALTY=0.073038
PRIMER_RIGHT_2_PENALTY=0.010294
PRIMER_LEFT_2_SEQUENCE=GCACTTTGAGCACAAGACATTC
PRIMER_RIGHT_2_SEQUENCE=ATATGGTGAGGATGCGTCTCTT
PRIMER_LEFT_2=202,22
PRIMER_RIGHT_2=437,22
PRIMER_LEFT_2_TM=59.927
PRIMER_RIGHT_2_TM=59.990
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.1031
PRIMER_LEFT_3_PENALTY=0.092792
PRIMER_RIGHT_3_PENALTY=0.010294
PRIMER_LEFT_3_SEQUENCE=CAACATCTTCTCCACGTCTCTG
PRIMER_RIGHT_3_SEQUENCE=ATATGGTGAGGATGCGTCTCTT
PRIMER_LEFT_3=291,22
PRIMER_RIGHT_3=437,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=59.990
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=0.1097
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.010294
PRIMER_LEFT_4_SEQUENCE=ACCTTGAGAGGGTGAACAACAT
PRIMER_RIGHT_4_SEQUENCE=ATATGGTGAGGATGCGTCTCTT
PRIMER_LEFT_4=275,22
PRIMER_RIGHT_4=437,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=59.990
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_02729 Ps_contig_mira-and-tgicl-ass_franssen_24351
SEQUENCE=TTGCGGGGATVARACACACAACATATTAAGTGAAGTGAATCATATATATATTAAATCTATATTCATCATCACTACTTAAGTTATGGAGAAGAAATCACTAGCTGCCTTGTCCTTCCTCTTCCTTTTCTCTTTGTTGCACAAGAAATTGTGGTGACAGAGGCAAACACCTGTGAGCATTTGGCAGATACATACAGGGGAGTATGCTTCACGGATGCTAGCTGTGATGATCACTGCAAGAACAAAGCGCACTTAATCAGTGGCACGTGCCATGACTGGAAATGTTTCTGCACTCAAAACTGTTAAAGACGTTATAAATATAATAATATAAATAAATAAAATGCATGCAGTTATAGACAACTACCGNTGTATCTAGTTGTTTAGTTAATTTAGAAATTAAGTTNTGTGT
TARGET=42,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02730 Ps_contig_mira-and-tgicl-ass_franssen_24351
SEQUENCE=TTGCGGGGATVARACACACAACATATTAAGTGAAGTGAATCATATATATATTAAATCTATATTCATCATCACTACTTAAGTTATGGAGAAGAAATCACTAGCTGCCTTGTCCTTCCTCTTCCTTTTCTCTTTGTTGCACAAGAAATTGTGGTGACAGAGGCAAACACCTGTGAGCATTTGGCAGATACATACAGGGGAGTATGCTTCACGGATGCTAGCTGTGATGATCACTGCAAGAACAAAGCGCACTTAATCAGTGGCACGTGCCATGACTGGAAATGTTTCTGCACTCAAAACTGTTAAAGACGTTATAAATATAATAATATAAATAAATAAAATGCATGCAGTTATAGACAACTACCGNTGTATCTAGTTGTTTAGTTAATTTAGAAATTAAGTTNTGTGT
TARGET=325,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02731 Ps_contig_mira-and-tgicl-ass_franssen_24358
SEQUENCE=GAAAGGAAGGAAGGGTGGTTTTCGGAGCTGCCGAGACCTTTTTATTTTCTTTTCCGGTTTCTAAAAGGTCCGGCGACGGTGTCATCCCACAAGTGATCGAGAAGGCTCATGAGAATATGGAAAAGCGCAAGACACC
TARGET=3,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02732 Ps_contig_mira-and-tgicl-ass_franssen_24361
SEQUENCE=AAGACAGTGGTAKWCAACGACAGAGTACGACATTSGCCCCCACCAAAACCCTTTCAAATTCACCTCTCTCTCTCTCTCTCTCTTCCGATTTTCGTTTTCTAGGTCATTTCAATTTCACGAAAATGAGAGAAATTCTTCATATCCAAGGTGGCCAATGCGGAAACCAGATCGGAGCCAAATTCTGGGAAGTTGTTTGCGCCGAACATGGAATCGACCCTACCGGAAGGTATGGTGGTGACACAGATCTTCAATTAGAGAGAATCAACGTCTATWACAACG
TARGET=64,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02733 Ps_contig_mira-and-tgicl-ass_franssen_24380
SEQUENCE=GATTTTCCAGTCCAATACTCGGGAGGATTCTTCCAATCTCGCCCATTCCACCGATTCATCAAAGAAGAAAGAAAGAGTGAGTGGATCCATCAAAATGAGCCATAGCATCTTTCACCAAACTTTGCTTTGTCAAACGCAATCGGTTGCTGAGCATCAAAGTAAGCTTGGTTCTCGTGGAATTGTTTGCAGTACTTTATTCCAATCGCAGAGTGTTGATAAAGAAAAGAAGCTTTTGCTCTCTACAAATTTCCGCGGGAGCAGATTGTGTGTTAGGAAACGCAGAGTAACCACTCTTAATTTCTCTTTTCCTTCCCTCTTCATTCACACACCCTTCAGCTTTTTTTCAATCCCTCTCACCTTT
TARGET=65,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2955
PRIMER_LEFT_PENALTY=0.164639
PRIMER_RIGHT_PENALTY=0.130883
PRIMER_LEFT_SEQUENCE=GATTTTCCAGTCCAATACTCGG
PRIMER_RIGHT_SEQUENCE=CAACACTCTGCGATTGGAATAA
PRIMER_LEFT=0,22
PRIMER_RIGHT=214,22
PRIMER_LEFT_TM=59.835
PRIMER_RIGHT_TM=60.131
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=215
PRIMER_PAIR_PENALTY_1=0.3482
PRIMER_LEFT_1_PENALTY=0.164639
PRIMER_RIGHT_1_PENALTY=0.183585
PRIMER_LEFT_1_SEQUENCE=GATTTTCCAGTCCAATACTCGG
PRIMER_RIGHT_1_SEQUENCE=GAGTGGTTACTCTGCGTTTCCT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=292,22
PRIMER_LEFT_1_TM=59.835
PRIMER_RIGHT_1_TM=59.816
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.3482
PRIMER_LEFT_2_PENALTY=0.164639
PRIMER_RIGHT_2_PENALTY=0.183585
PRIMER_LEFT_2_SEQUENCE=GATTTTCCAGTCCAATACTCGG
PRIMER_RIGHT_2_SEQUENCE=AGAGTGGTTACTCTGCGTTTCC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=59.835
PRIMER_RIGHT_2_TM=59.816
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=294
PRIMER_PAIR_PENALTY_3=0.4721
PRIMER_LEFT_3_PENALTY=0.164639
PRIMER_RIGHT_3_PENALTY=0.307443
PRIMER_LEFT_3_SEQUENCE=GATTTTCCAGTCCAATACTCGG
PRIMER_RIGHT_3_SEQUENCE=GCTATGGCTCATTTTGATGGAT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=105,22
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=60.307
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.4880
PRIMER_LEFT_4_PENALTY=0.164639
PRIMER_RIGHT_4_PENALTY=0.323355
PRIMER_LEFT_4_SEQUENCE=GATTTTCCAGTCCAATACTCGG
PRIMER_RIGHT_4_SEQUENCE=TGCGTTTCCTAACACACAATCT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=280,22
PRIMER_LEFT_4_TM=59.835
PRIMER_RIGHT_4_TM=59.677
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_02734 Ps_contig_mira-and-tgicl-ass_franssen_24386
SEQUENCE=GTTCGGGAACCACACATCAATTAACTCCTAGATAACACACACACAACACAAAAATGGGTAAAGATGTTGAGGTTCAAGAACAAGGTGCTGAATTCTCAGCTAAGGATTACCAAGATCCACCACCAGCACCACTTTTTGATTCAGCTGAAGTTTAAAAAATGGTCTCTGTACAGAGCTGTTATAGCAGTAGTTTGTAGCAACTTCTTTCTTTTTCTTACATCACTTGTTTTGACCTATTTATTAGGTTAAAAA
TARGET=35,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6471
PRIMER_LEFT_PENALTY=1.363410
PRIMER_RIGHT_PENALTY=0.283676
PRIMER_LEFT_SEQUENCE=CGGGAACCACACATCAATTAAC
PRIMER_RIGHT_SEQUENCE=TCAGCTGAATCAAAAAGTGGTG
PRIMER_LEFT=3,22
PRIMER_RIGHT=147,22
PRIMER_LEFT_TM=61.363
PRIMER_RIGHT_TM=60.284
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=1.6553
PRIMER_LEFT_1_PENALTY=1.371663
PRIMER_RIGHT_1_PENALTY=0.283676
PRIMER_LEFT_1_SEQUENCE=GGAACCACACATCAATTAACTCC
PRIMER_RIGHT_1_SEQUENCE=TCAGCTGAATCAAAAAGTGGTG
PRIMER_LEFT_1=5,23
PRIMER_RIGHT_1=147,22
PRIMER_LEFT_1_TM=59.628
PRIMER_RIGHT_1_TM=60.284
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=1.6553
PRIMER_LEFT_2_PENALTY=1.371663
PRIMER_RIGHT_2_PENALTY=0.283676
PRIMER_LEFT_2_SEQUENCE=GGGAACCACACATCAATTAACTC
PRIMER_RIGHT_2_SEQUENCE=TCAGCTGAATCAAAAAGTGGTG
PRIMER_LEFT_2=4,23
PRIMER_RIGHT_2=147,22
PRIMER_LEFT_2_TM=59.628
PRIMER_RIGHT_2_TM=60.284
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=1.6660
PRIMER_LEFT_3_PENALTY=1.363410
PRIMER_RIGHT_3_PENALTY=0.302618
PRIMER_LEFT_3_SEQUENCE=CGGGAACCACACATCAATTAAC
PRIMER_RIGHT_3_SEQUENCE=AGCTGAATCAAAAAGTGGTGCT
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=145,22
PRIMER_LEFT_3_TM=61.363
PRIMER_RIGHT_3_TM=60.303
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=1.6743
PRIMER_LEFT_4_PENALTY=1.371663
PRIMER_RIGHT_4_PENALTY=0.302618
PRIMER_LEFT_4_SEQUENCE=GGAACCACACATCAATTAACTCC
PRIMER_RIGHT_4_SEQUENCE=AGCTGAATCAAAAAGTGGTGCT
PRIMER_LEFT_4=5,23
PRIMER_RIGHT_4=145,22
PRIMER_LEFT_4_TM=59.628
PRIMER_RIGHT_4_TM=60.303
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_02735 Ps_contig_mira-and-tgicl-ass_franssen_24391
SEQUENCE=GTTGTTAGTGGTGCTCCTCAAACCAAGAAGTTGCAAGACATACTTCCTAGCATTATCCACCAATTAGGGCCGGATAATTTGGAAAACCTGAAGAAGATAGCTGAGCAGTTCAATAAGCAGGTTCCTGAAGCAGGTGCTGGCACAGCCACAGCACAAGAAGAGAATGATGATGATGATGTTCCAGAGCTTGTCCCAGGAGAGACCTTTGAGACAGCTGCTGAGGAGGCCAAGGATAGTTAGTGTTATTGTTGTTTTTTTTTCAACAAAGAAAATCTTGATTTTCAATTTTAAGTGTTTTAGGTTGTTTTGTCCTCTTGTCATATACTTTAAACAGTCTCAATACTTGAATTTAATCTGTTTTCCTTTGTACTAAATTAAGTATTACTTCTATGCGCTAGATTTCTGCCAAATTTTAAGTATTTCTGTAAGTGACAAGAATTAAATTTCTTAAAAATTTCATTCAGTRA
TARGET=164,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02736 Ps_contig_mira-and-tgicl-ass_franssen_24427
SEQUENCE=TCCATGGTTCTTGTTATTTCCTCCGAGGTTTCAGCTAGAGAATTAGCTGAGACTTCCACTAATGCCAAAGAGGAAGTTGCTGAAAAGTCAAATGAAGTAAATGATGCCAAATATTACGGTGGAGGTTATGGTCATGGCTATGGTCATGGACATGGAGGCTACGGTCATGGAKKCGKRCGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGAACGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCGAACAATGAGGTTGTTAGAAAGTCAAATGAAGTAATGATGCCAATATGGCGGTGGACA
TARGET=232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02737 Ps_contig_mira-and-tgicl-ass_franssen_24427
SEQUENCE=TCCATGGTTCTTGTTATTTCCTCCGAGGTTTCAGCTAGAGAATTAGCTGAGACTTCCACTAATGCCAAAGAGGAAGTTGCTGAAAAGTCAAATGAAGTAAATGATGCCAAATATTACGGTGGAGGTTATGGTCATGGCTATGGTCATGGACATGGAGGCTACGGTCATGGAKKCGKRCGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGAACGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGATACAACGGTGGCGGTGTTTCCGAACAATGAGGTTGTTAGAAAGTCAAATGAAGTAATGATGCCAATATGGCGGTGGACA
TARGET=256,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02738 Ps_contig_mira-and-tgicl-ass_franssen_24445
SEQUENCE=CATATCCTCCTCCTCCACTACCGTATCCTCCTCCTCCACCTTACCACCACTAGATAGTCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCTAGTTGTTGCATGAATTGTWAATTTCAAAGTTTCGGTTTTARTCAAGCAAGCCAAATAATATATCCTNTATTATAGTAATTTCG
TARGET=5,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02739 Ps_contig_mira-and-tgicl-ass_franssen_24445
SEQUENCE=CATATCCTCCTCCTCCACTACCGTATCCTCCTCCTCCACCTTACCACCACTAGATAGTCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCTAGTTGTTGCATGAATTGTWAATTTCAAAGTTTCGGTTTTARTCAAGCAAGCCAAATAATATATCCTNTATTATAGTAATTTCG
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02740 Ps_contig_mira-and-tgicl-ass_franssen_24466
SEQUENCE=AGTCGGGGAGGGTTCTCATCTTTTGGTGTTGAGTTAGTTTCTCTCTGGTCTTTTCTTTTTTCTTTTTATCTATTTGAAGAAGATGGATCTGTACTTGAAGATGTGATTTATAAGTGGTCTTGGCAATGATTGTGATAATTTAACTTGTAAAAATTCATAATGTAATGTAACGGCTTACATTATTTATTATTATTATTATGTTGATATAACTTTATAGAATAACTTTCGAGGTTGAATT
TARGET=184,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02741 Ps_contig_mira-and-tgicl-ass_franssen_24472
SEQUENCE=TTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCAAAAATCACTCATTACCGCGAACCCCCACAATCTACTACCTTCATCCGCTGCGCATCACCACACCCCACAACATCCATCCACACCTCCGCCAAATCTTCCGACAACAACGTCCAAACCCAGACTTTTTTATAAACTGTTTCGCCGGCGACCACCATCACCGCGAACACGCCGACACCCTTTCACCGCCGCTGCTTCGCCACAACTTCTCGCTCTTCTACAACAAAAA
TARGET=65,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8483
PRIMER_LEFT_PENALTY=0.621889
PRIMER_RIGHT_PENALTY=0.226380
PRIMER_LEFT_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_SEQUENCE=GCAGCGGATGAAGGTAGTAGAT
PRIMER_LEFT=3,22
PRIMER_RIGHT=151,22
PRIMER_LEFT_TM=59.378
PRIMER_RIGHT_TM=59.774
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=0.8560
PRIMER_LEFT_1_PENALTY=0.621889
PRIMER_RIGHT_1_PENALTY=0.234069
PRIMER_LEFT_1_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_1_SEQUENCE=TGAAGGTAGTAGATTGTGGGGG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=143,22
PRIMER_LEFT_1_TM=59.378
PRIMER_RIGHT_1_TM=60.234
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=12.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.8659
PRIMER_LEFT_2_PENALTY=0.621889
PRIMER_RIGHT_2_PENALTY=0.243965
PRIMER_LEFT_2_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_2_SEQUENCE=GCGGTAATGAGTGATTTTTGGT
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=118,22
PRIMER_LEFT_2_TM=59.378
PRIMER_RIGHT_2_TM=60.244
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=0.9944
PRIMER_LEFT_3_PENALTY=0.621889
PRIMER_RIGHT_3_PENALTY=0.372557
PRIMER_LEFT_3_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_3_SEQUENCE=GATTTTTGGTGGTGGTGGTTAC
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=106,22
PRIMER_LEFT_3_TM=59.378
PRIMER_RIGHT_3_TM=60.373
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=104
PRIMER_PAIR_PENALTY_4=1.1385
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=0.516652
PRIMER_LEFT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=ATGAGTGATTTTTGGTGGTGGT
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=112,22
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=60.517
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_02742 Ps_contig_mira-and-tgicl-ass_franssen_24472
SEQUENCE=TTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCAAAAATCACTCATTACCGCGAACCCCCACAATCTACTACCTTCATCCGCTGCGCATCACCACACCCCACAACATCCATCCACACCTCCGCCAAATCTTCCGACAACAACGTCCAAACCCAGACTTTTTTATAAACTGTTTCGCCGGCGACCACCATCACCGCGAACACGCCGACACCCTTTCACCGCCGCTGCTTCGCCACAACTTCTCGCTCTTCTACAACAAAAA
TARGET=89,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8483
PRIMER_LEFT_PENALTY=0.621889
PRIMER_RIGHT_PENALTY=0.226380
PRIMER_LEFT_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_SEQUENCE=GCAGCGGATGAAGGTAGTAGAT
PRIMER_LEFT=3,22
PRIMER_RIGHT=151,22
PRIMER_LEFT_TM=59.378
PRIMER_RIGHT_TM=59.774
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=0.8560
PRIMER_LEFT_1_PENALTY=0.621889
PRIMER_RIGHT_1_PENALTY=0.234069
PRIMER_LEFT_1_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_1_SEQUENCE=TGAAGGTAGTAGATTGTGGGGG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=143,22
PRIMER_LEFT_1_TM=59.378
PRIMER_RIGHT_1_TM=60.234
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=12.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=1.3617
PRIMER_LEFT_2_PENALTY=1.135283
PRIMER_RIGHT_2_PENALTY=0.226380
PRIMER_LEFT_2_SEQUENCE=ACAACCTCATTGTTGGAAACAC
PRIMER_RIGHT_2_SEQUENCE=GCAGCGGATGAAGGTAGTAGAT
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=151,22
PRIMER_LEFT_2_TM=58.865
PRIMER_RIGHT_2_TM=59.774
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=1.3694
PRIMER_LEFT_3_PENALTY=1.135283
PRIMER_RIGHT_3_PENALTY=0.234069
PRIMER_LEFT_3_SEQUENCE=ACAACCTCATTGTTGGAAACAC
PRIMER_RIGHT_3_SEQUENCE=TGAAGGTAGTAGATTGTGGGGG
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=143,22
PRIMER_LEFT_3_TM=58.865
PRIMER_RIGHT_3_TM=60.234
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=12.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=1.6447
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=1.022788
PRIMER_LEFT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=ACGTTGTTGTCGGAAGATTTG
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=210,21
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=60.023
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_02743 Ps_contig_mira-and-tgicl-ass_franssen_24482
SEQUENCE=CACAACAAGTCACCAGGATACTATGTATTGGAAGATACTGGACAATRTAGGAAGCTTCCTATGTTTGGTACCACTGATGCTTCTCAAGTCTTGAAGGAGCTTGATGAAGTTGTTGCCGCTTACCCTCAAGCTTTCGTTCGTATCATCGGTTTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCNTTGCACACACACAGAATCCTACTAAGTTCACTGCTAGTTTGGGACGTCCTTTCCTTTAATTTTAATTATTAGG
TARGET=191,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02744 Ps_contig_mira-and-tgicl-ass_franssen_24490
SEQUENCE=GACTYTATGCCATTGCTTCATATTCTTGCTCACCTTCAACTCCTATTGGCTATAATTGTTTTTCTTCATTTATATGGAATAATTTACTACTAGAATCCTACAATACAAATACAAGATCAGTTGACAAAAAATTTTATATCATGTTATCCAACTCACCTCTCTCTATATATAACTAGTNTTCTGGTGTCTGTTTATTAGTTACTGCACACACACACACACAACATAA
TARGET=205,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02745 Ps_contig_mira-and-tgicl-ass_franssen_24523
SEQUENCE=AWTTGGAAGRAATTCTCTTAGACAAATCAGCAGAATGGATTATATAAAAGAAAGGCAATGTTCTAACAACAAGGATGATATTTGATATATTACACATGATAACATAGTGATTTATCTATATACTCTTTTGTTGAAAACCCAACAGTATTTATTCATGATTACACATAGTACATGCATGCATGGTGACATAATCAGTTACCATTGTTGGAACTACCACCATGTCCACCTCCACCACCATAACCACCACCACCATGGTTGTATCCTCCTCCACCACCTCCATGGTTGTAGCCTCCTCCGCCACCATTGTAACCACCACCTCCATGGTTGTAGCCTCCACCGCCACCGTTGTAACCACCACCTCCATGGTTGTATCCGCCTCCACCTCCATGGTTGTATCCTCCTCCACCACCATTGTAACCACCACCTCCATGGTTGTATCCTCCTCCGCCACCATTGTAACCACCACCTCCATGGTTGTATCCGCCTCCACCACCATGGTTGTAGCCTCCTCCACCACCGTTGTAACCTCCGCCGCCACCATGGTTGTAACCTCCACCGTAATATTTGGCATCATTTAATTCATCTGACTTTTCAACAACGTCATCTTTGGTATTAGTTGAAGTCTCAGTTAAATCCCTAGCTGAAACCTCTGAGGAAATAACAAGAACCATGGCCATTAGGCCAAGGATGAGAATTGCTATTTTGGAATCCATTGTACTCAACTCAAGCTCAACTGAAATGTGATATTTGGTAGTATTGGATCCCCGAC
TARGET=171,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02746 Ps_contig_mira-and-tgicl-ass_franssen_24523
SEQUENCE=AWTTGGAAGRAATTCTCTTAGACAAATCAGCAGAATGGATTATATAAAAGAAAGGCAATGTTCTAACAACAAGGATGATATTTGATATATTACACATGATAACATAGTGATTTATCTATATACTCTTTTGTTGAAAACCCAACAGTATTTATTCATGATTACACATAGTACATGCATGCATGGTGACATAATCAGTTACCATTGTTGGAACTACCACCATGTCCACCTCCACCACCATAACCACCACCACCATGGTTGTATCCTCCTCCACCACCTCCATGGTTGTAGCCTCCTCCGCCACCATTGTAACCACCACCTCCATGGTTGTAGCCTCCACCGCCACCGTTGTAACCACCACCTCCATGGTTGTATCCGCCTCCACCTCCATGGTTGTATCCTCCTCCACCACCATTGTAACCACCACCTCCATGGTTGTATCCTCCTCCGCCACCATTGTAACCACCACCTCCATGGTTGTATCCGCCTCCACCACCATGGTTGTAGCCTCCTCCACCACCGTTGTAACCTCCGCCGCCACCATGGTTGTAACCTCCACCGTAATATTTGGCATCATTTAATTCATCTGACTTTTCAACAACGTCATCTTTGGTATTAGTTGAAGTCTCAGTTAAATCCCTAGCTGAAACCTCTGAGGAAATAACAAGAACCATGGCCATTAGGCCAAGGATGAGAATTGCTATTTTGGAATCCATTGTACTCAACTCAAGCTCAACTGAAATGTGATATTTGGTAGTATTGGATCCCCGAC
TARGET=240,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02747 Ps_contig_mira-and-tgicl-ass_franssen_24524
SEQUENCE=TAACGAGAAGAATAGTGNGAAGATGCAAGTAGTGCCNTGAAACAATTTWACTTCGTTTCATATTTTAGACGCCTTTTTAATTTGCACTTTATAATAATGGTCGATGGTACCCTTGATTTGTTTGGACTTCTAGTTTTTAGCTCTTCGATTGTGTTATATTTTTTTAATTTTTCATCATTGTGTGCTTTTGCATTGTATCACAATCTTCTACTTTTTATGTTGTTGATATTGTTGTCGTCATTGTCGTTTTGTCATCATTTTTAGTTGTAATTTCTTTTTTCCCTAGGAGAGTCGTTTCTACGAAATTATTTTTCTTGCTAGTTCAATTTATTTTTAGGTTTATTGTTTATGTTTGTTTGTTATGTGGTTGAAATAAATTATAATATGATGTATACTTTTGACAAGTGTAACCAAAATATTGTCGAGTAATAATTTAAATTCTTGTASTCCAAT
TARGET=350,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02748 Ps_contig_mira-and-tgicl-ass_franssen_24530
SEQUENCE=AAGCAGTGGTATCAACGTTCAGAGTAWCGCGGGAGGCAAACAAACACCAGAACAAAACCAAGCAATACCCAACACACAGTTTGCTGCAATGGCCTCTGCTCAGTGCTACAGAACCTGTGAACAAAGCTGCCAACACAAAAACCACCACCACCAGCAGCAACATAGCTCAATCGGACAGAAGGTAACTGACTTCTACAAAGGACACCACAATGATGGAACACAATACTACCAGCAGCAGCAGCAACATAGCTCAATCGGACATAAGGCTGACTTCTACAAAGGACACCACAATGATGGAACACAAACCAAAACCGAATACTATAGCGAGACAGATGTGATTTATCAACCCGGATATGTTGCCAAAAATCAAAGTAACACATGCAACCAAACACGTCACAATCACAATCACGCCGCTACTGGCACCACCATTGTTACCGGCACCACAGCGGAAAAATGCCAAGGCAGAAACAGGAGGGAGAGGAGGAACATGTTCCAGAGGATCAAGGATGGCATATCAGGTCACAGCAGTGACAGTGGCAGTAGCAGTGATGAGTGTGACAGCGACAATGAAAACCGTCGCCACAGGAAGAACTGCAATTGAGGAGCAGCTGTTCCAATAATAATAAATTGGAGTTTGAGGATCGAAGTTGGCAACTCAAAGTCCAATATTATGTGTGTGATGATTGCTGGCAAGTTTGAATAAATTAGCTACTATAAGTATTAATATAG
TARGET=141,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02749 Ps_contig_mira-and-tgicl-ass_franssen_24530
SEQUENCE=AAGCAGTGGTATCAACGTTCAGAGTAWCGCGGGAGGCAAACAAACACCAGAACAAAACCAAGCAATACCCAACACACAGTTTGCTGCAATGGCCTCTGCTCAGTGCTACAGAACCTGTGAACAAAGCTGCCAACACAAAAACCACCACCACCAGCAGCAACATAGCTCAATCGGACAGAAGGTAACTGACTTCTACAAAGGACACCACAATGATGGAACACAATACTACCAGCAGCAGCAGCAACATAGCTCAATCGGACATAAGGCTGACTTCTACAAAGGACACCACAATGATGGAACACAAACCAAAACCGAATACTATAGCGAGACAGATGTGATTTATCAACCCGGATATGTTGCCAAAAATCAAAGTAACACATGCAACCAAACACGTCACAATCACAATCACGCCGCTACTGGCACCACCATTGTTACCGGCACCACAGCGGAAAAATGCCAAGGCAGAAACAGGAGGGAGAGGAGGAACATGTTCCAGAGGATCAAGGATGGCATATCAGGTCACAGCAGTGACAGTGGCAGTAGCAGTGATGAGTGTGACAGCGACAATGAAAACCGTCGCCACAGGAAGAACTGCAATTGAGGAGCAGCTGTTCCAATAATAATAAATTGGAGTTTGAGGATCGAAGTTGGCAACTCAAAGTCCAATATTATGTGTGTGATGATTGCTGGCAAGTTTGAATAAATTAGCTACTATAAGTATTAATATAG
TARGET=230,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02750 Ps_contig_mira-and-tgicl-ass_franssen_24549
SEQUENCE=AAGTAYMACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTTGCTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTTCACTTCTTCAACCGATTCCTCAGTTTCTTTAGGTGCTTCTAAGTTCTCTTCCTTTGCTTCCTCTGTTACCACTTCCTTGTTCTCAACTTCAAGTGGATCTTGGGTTTCGGGAGCATCTACAGCTTCTCCTGCTGCTAGTTTGTTTCTTGGTTTGGGTTTTTCGGTTGTTTCTTCKTGKTGGTTACTTCGGTGGCAGGTAACTTTCGGATTTCGTGGGTTTGTCGCCCGNAGCGAGGGTACTTCG
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02751 Ps_contig_mira-and-tgicl-ass_franssen_24552
SEQUENCE=AAAACAAAAGGTAGACTTTGTATGAAGCACCGTCGGAAACCAAAGTTGAAGAGGAAGGTGAAAAGAAACACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTCAAGTGAAGAGGAAGGAGAAGATGGGTGAAGAAAAAGAAAGAGAAGAAGGAAANAAGAAGAAGAAGACATCGTGCNGTGAGAAGT
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02752 Ps_contig_mira-and-tgicl-ass_franssen_24553
SEQUENCE=GTTCGRKGAACACAAACCTAAGCATTTCAACTATTTATATATATATTCACAGAGATCATATCGTATATCATAAGATGACTTGGTTTTATTCAGACAACTATTCGTTTGGGTGGGTACGAGTGGTAGGAAAACAAGTGGAACTTGTTGATGACTAATTWAACAACAAGTGTGGCTATACAAGTATTAGTTGGGTGGAGGCTAGACATAC
TARGET=36,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02753 Ps_contig_mira-and-tgicl-ass_franssen_24571
SEQUENCE=CGTTTTTTACCAGTTCCAGTCACACCCATTCCATTGGTTTAAGCTTCAGGATTCTCAACTGGAACTTCCTCTGCTGCTGGAGCTTCCACTGATTCAACTGTTTTGTTCTCAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTTGCTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTCACTTCTCAACCGATTCCTCTAGTTTTCTTTAGGTGTCTTCTAAGTTCTCTTCCTTGCTTCTNTCTGTTACCAC
TARGET=132,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2665
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.246726
PRIMER_LEFT_SEQUENCE=ACACCCATTCCATTGGTTTAAG
PRIMER_RIGHT_SEQUENCE=ATCGGTTGAGAAGTGAGGAAGA
PRIMER_LEFT=21,22
PRIMER_RIGHT=216,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.247
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.2665
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.246726
PRIMER_LEFT_1_SEQUENCE=ACACCCATTCCATTGGTTTAAG
PRIMER_RIGHT_1_SEQUENCE=GAGGAATCGGTTGAGAAGTGAG
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=221,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=60.247
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.2665
PRIMER_LEFT_2_PENALTY=0.019805
PRIMER_RIGHT_2_PENALTY=0.246726
PRIMER_LEFT_2_SEQUENCE=ACACCCATTCCATTGGTTTAAG
PRIMER_RIGHT_2_SEQUENCE=AGAGGAATCGGTTGAGAAGTGA
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=222,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=60.247
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.2686
PRIMER_LEFT_3_PENALTY=0.019805
PRIMER_RIGHT_3_PENALTY=0.248820
PRIMER_LEFT_3_SEQUENCE=ACACCCATTCCATTGGTTTAAG
PRIMER_RIGHT_3_SEQUENCE=AATCGGTTGAGAAGTGAGGAAG
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=217,22
PRIMER_LEFT_3_TM=59.980
PRIMER_RIGHT_3_TM=59.751
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=0.2995
PRIMER_LEFT_4_PENALTY=0.052729
PRIMER_RIGHT_4_PENALTY=0.246726
PRIMER_LEFT_4_SEQUENCE=CTCAACTGGAACTTCCTCTGCT
PRIMER_RIGHT_4_SEQUENCE=GAGGAATCGGTTGAGAAGTGAG
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=221,22
PRIMER_LEFT_4_TM=60.053
PRIMER_RIGHT_4_TM=60.247
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=169
=
PRIMER_SEQUENCE_ID=SSR_02754 Ps_contig_mira-and-tgicl-ass_franssen_24586
SEQUENCE=AACCAAGAAGTTGCAGGACATACTTCCTAGCATTATCCACCAGTTAGGGCCAGATAATTTGGAAAACCTGAAGAAGTTAGCCGAGCAGTTCAATAAGCAGGTTCCTGAAGCAGGTGCTGGCACAGCCGCCACAGCACAAGAAGAGGATGATGATGATGTTCCAGAGCTTGTCCCAGGAGAGACTTTTGAGACAGCCGCGGAGGAGGCCAAGGATAGTTAGTGTGTTATTGTGGCCATCTTGTGTTTTTTCAACAAAGAAAATCTTGATTTTCAATTTTGTATTTTAGGTTGTTTTGTTCTCATGTCATATTATTTAAACAGTCTGCATACTTGAATTTAATCTGTTTTCCTTTGTCTAAAACAAGTATTACTTCTATGCTCTAGATTTCTACCAAATTCTATGTATTTCTGTAAGTGACATGAGAATTAAATTTCTTAAAATTTCATTCAGTACAGCACATCACCTTCTTGTGAATCTGGTTTGCAATACTGAATTCACAAGGATGTATTATCGTTTTAAATCTTGTT
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2248
PRIMER_LEFT_PENALTY=0.080887
PRIMER_RIGHT_PENALTY=0.143960
PRIMER_LEFT_SEQUENCE=GAAGTTAGCCGAGCAGTTCAAT
PRIMER_RIGHT_SEQUENCE=TCTCAAAAGTCTCTCCTGGGAC
PRIMER_LEFT=72,22
PRIMER_RIGHT=190,22
PRIMER_LEFT_TM=59.919
PRIMER_RIGHT_TM=59.856
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.3004
PRIMER_LEFT_1_PENALTY=0.156479
PRIMER_RIGHT_1_PENALTY=0.143960
PRIMER_LEFT_1_SEQUENCE=GCCAGATAATTTGGAAAACCTG
PRIMER_RIGHT_1_SEQUENCE=TCTCAAAAGTCTCTCCTGGGAC
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=190,22
PRIMER_LEFT_1_TM=59.844
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.3494
PRIMER_LEFT_2_PENALTY=0.205455
PRIMER_RIGHT_2_PENALTY=0.143960
PRIMER_LEFT_2_SEQUENCE=CTGAAGAAGTTAGCCGAGCAGT
PRIMER_RIGHT_2_SEQUENCE=TCTCAAAAGTCTCTCCTGGGAC
PRIMER_LEFT_2=67,22
PRIMER_RIGHT_2=190,22
PRIMER_LEFT_2_TM=60.205
PRIMER_RIGHT_2_TM=59.856
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=0.3541
PRIMER_LEFT_3_PENALTY=0.080887
PRIMER_RIGHT_3_PENALTY=0.273230
PRIMER_LEFT_3_SEQUENCE=GAAGTTAGCCGAGCAGTTCAAT
PRIMER_RIGHT_3_SEQUENCE=CAAAAGTCTCTCCTGGGACAAG
PRIMER_LEFT_3=72,22
PRIMER_RIGHT_3=187,22
PRIMER_LEFT_3_TM=59.919
PRIMER_RIGHT_3_TM=60.273
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=116
PRIMER_PAIR_PENALTY_4=0.3548
PRIMER_LEFT_4_PENALTY=0.210886
PRIMER_RIGHT_4_PENALTY=0.143960
PRIMER_LEFT_4_SEQUENCE=AGCAGTTCAATAAGCAGGTTCC
PRIMER_RIGHT_4_SEQUENCE=TCTCAAAAGTCTCTCCTGGGAC
PRIMER_LEFT_4=83,22
PRIMER_RIGHT_4=190,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=59.856
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_02755 Ps_contig_mira-and-tgicl-ass_franssen_24587
SEQUENCE=AAAAAAAWWWWTATTAATTTGATTATGGATAATCGATTTTTTTCTACAACCCTATTCATGTCTAATCATGTGGGTGTGGACACTTATTGAAGGGCTATCCAAACGCCATTATTCAAATGCTGTTCAAATTGTTTTAGACCACAGGTAGAGTTTGAATGAGAATGACTTTTCAATTAAGCAATTTATGCACTTCAATCAAATGTCATTTGTTCAGCTTCATGCCGGATTGATTCAAAAAGTTCTGAAGACAGATAACGTTCTCCAAAACTGGGAAAAATCGCAACGATAAGCTTTCCTGCATTTTCTGGTCTCTTCCCCAATTTTATTGCAGCTGCAGCAGCAGCACCGGATGAAATTCCCATCAGTAAACCTTCTTTCAAAGCAAGCAACTTAGCAGTTTCTATAGCTTCTTCACTTGAAATCTGAATAACTTCATCTAGAAAATCAAATTCCAGAATAGGGGGAACGATACCGGCACCGAGCCCTTGGATTAGATGTTCGCCTGGCTTTCCTCCATTCAGAACCGCACTTTCAGCTGGTTCCACACCATACACCTTGATTTCTGAGTTTTTCTCCTTGAGGAACTTCCCTGTACCTGTTACAGTTCCCCCGGTTCCTATCCCTGCAACCAAAGCATCAATTTTCCCTCCAGAGTCTCTCCAAATCTCAGGGCCAGTTGTCTCATAATGAATCTTCGCATTGACAGGGTTTTCAAATTGATTGAGCTTATAACTATTAGGCGTCTTCGCTAATAGCTCTTCCGCTTTCTCAAAAACACCAACAAATCCTTTGGCAGGATCTGTCAAATATACCTCAGCTCCAAATGCTCGCAGTATAATTCTTCTCTCAAGACTCATATTAGATGGCATAGCCACTATAAGTTTATAGCCTCTCAGCGCTGCTATCGATGCCAGTCCTATGCCGGTGTTACCACCTGTAGCCTCAACAAGGACAGTCTTTCCAGGTGTAATGAGGCCTTTGTTTTCAGCATCTTCAATCATGCTTAATGATATTCTATCTTTAACACTGCAGCAGGATTGCAAGTATTCTAGCTTAGCAGCGATTCGAGCAACACAGCCTTCAGTAATGTTGTTAAGATATACCAATGGAGTATTCCCTATTAATTCAGTAGCATCTTTCTTGATTGCAAACTGTGTCTCCATGATATCTGATGAAGAAAGCCAATGTCTTCAAAGGCGGTTTTGGATTGTTGAAAAAGCCGSCRATTTWAGCTTGASCGTMYSYGTATTTAAGAGCTATTAGAATTTTTAGAATC
TARGET=334,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02756 Ps_contig_mira-and-tgicl-ass_franssen_24593
SEQUENCE=GTCGGGGBGATCTTAATTTAATGTTTTTCTCCCTCACCCTCTCATCTAAGGTTTCTCTATAACTACACAAAGAGAGAAAAAAATTCATATCAATTAGTATATATATATATCACACTCTTTTGTTGCCAGCAAGATTTCTAGAAATGGGTGAAGAGAAGAAGGAAGAAGGAAAGAAAGAAGAAAGTAAAGAGGAAAAGAAGGAAGAAGAGAAAAAGGAAGAAAAGAAAGATGAAGAACAACTTCCTCAAGAGATAGTACTCAAAGTTGATATGCATTGTGAAGCTTGTGCAAGGAAAGTTGCAAAAGCATTGAAAGGATTTGAAGGAGTGGAGGAGGTGAGTGCAGATAGTAAGAGCAGCAAAGTGGGTAGTGAAAGGAAAGGCAGCAGACCCCATGAAAGTACTCAAAAGACTACAAAAGAAAAGTGGTAAGAAAGTGGAACTTATTTCACCTTTGCCTAAACCTCCACAAGACAAAAAAGAAGAAGAAGAGATCAAAGAACCACCAAAGCCAGAAGAGAAAAAAGATGAGGGTCCTACTGTGGTAACAATAGTTCTTAAAATAAGAATGCATTGTGATGCATGTGCTCAAGTTATACAGAAGAGAATCCGTAAGATAAAAGGAGTAGAATCAGTGGAAACAGATTTAGGGAACGACCAAGCCATAGTAAAAGGTGTGATTGATCCAGCAAAGCTTGTCGATGAAGTATACAAGAGAACCAAAAAGCAAGCTTCAATAGTGAAAGAGGAGGAAGAAAAGAAGGAAGAAGAGAAGAAAGAAGAGGAGAAAAGGAAGAGAAAAAGAAGGAGAAGAAGAAGAGAAAAAAGAGAGTGAGGAAGAAAACAAGGAAGAAGATGATAATAAAACTGAAATCAAGCGTAGTGAATATTGGCCATCAAAAGATTATATTGATTATGCTTATGCACCTGAGATTTTCAGTGATGAAAATCCAAATGCTTGTTCTGTTATGTAGACTTGTTGTTATTTGTAATGTTTCTGTATTTTAATAGCTTATTAGATTAAGTTAATTTTCTATTTCTATGTAACATAGTATAGTAGTGAATATTTTAAGGTTTTTAGCTTTCTGTTGTGTTTATGAGCTTTCAATGAATTTCACTGTCAAAAGATGGCAGAATCACTTATGTTTCTGTGTATGTGTTAAAGTGTACTGATACTGAATTTTGGGCTGCAATTTATTATRKTACCTATAATGTTGAACG
TARGET=98,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02757 Ps_contig_mira-and-tgicl-ass_franssen_24593
SEQUENCE=GTCGGGGBGATCTTAATTTAATGTTTTTCTCCCTCACCCTCTCATCTAAGGTTTCTCTATAACTACACAAAGAGAGAAAAAAATTCATATCAATTAGTATATATATATATCACACTCTTTTGTTGCCAGCAAGATTTCTAGAAATGGGTGAAGAGAAGAAGGAAGAAGGAAAGAAAGAAGAAAGTAAAGAGGAAAAGAAGGAAGAAGAGAAAAAGGAAGAAAAGAAAGATGAAGAACAACTTCCTCAAGAGATAGTACTCAAAGTTGATATGCATTGTGAAGCTTGTGCAAGGAAAGTTGCAAAAGCATTGAAAGGATTTGAAGGAGTGGAGGAGGTGAGTGCAGATAGTAAGAGCAGCAAAGTGGGTAGTGAAAGGAAAGGCAGCAGACCCCATGAAAGTACTCAAAAGACTACAAAAGAAAAGTGGTAAGAAAGTGGAACTTATTTCACCTTTGCCTAAACCTCCACAAGACAAAAAAGAAGAAGAAGAGATCAAAGAACCACCAAAGCCAGAAGAGAAAAAAGATGAGGGTCCTACTGTGGTAACAATAGTTCTTAAAATAAGAATGCATTGTGATGCATGTGCTCAAGTTATACAGAAGAGAATCCGTAAGATAAAAGGAGTAGAATCAGTGGAAACAGATTTAGGGAACGACCAAGCCATAGTAAAAGGTGTGATTGATCCAGCAAAGCTTGTCGATGAAGTATACAAGAGAACCAAAAAGCAAGCTTCAATAGTGAAAGAGGAGGAAGAAAAGAAGGAAGAAGAGAAGAAAGAAGAGGAGAAAAGGAAGAGAAAAAGAAGGAGAAGAAGAAGAGAAAAAAGAGAGTGAGGAAGAAAACAAGGAAGAAGATGATAATAAAACTGAAATCAAGCGTAGTGAATATTGGCCATCAAAAGATTATATTGATTATGCTTATGCACCTGAGATTTTCAGTGATGAAAATCCAAATGCTTGTTCTGTTATGTAGACTTGTTGTTATTTGTAATGTTTCTGTATTTTAATAGCTTATTAGATTAAGTTAATTTTCTATTTCTATGTAACATAGTATAGTAGTGAATATTTTAAGGTTTTTAGCTTTCTGTTGTGTTTATGAGCTTTCAATGAATTTCACTGTCAAAAGATGGCAGAATCACTTATGTTTCTGTGTATGTGTTAAAGTGTACTGATACTGAATTTTGGGCTGCAATTTATTATRKTACCTATAATGTTGAACG
TARGET=479,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02758 Ps_contig_mira-and-tgicl-ass_franssen_24593
SEQUENCE=GTCGGGGBGATCTTAATTTAATGTTTTTCTCCCTCACCCTCTCATCTAAGGTTTCTCTATAACTACACAAAGAGAGAAAAAAATTCATATCAATTAGTATATATATATATCACACTCTTTTGTTGCCAGCAAGATTTCTAGAAATGGGTGAAGAGAAGAAGGAAGAAGGAAAGAAAGAAGAAAGTAAAGAGGAAAAGAAGGAAGAAGAGAAAAAGGAAGAAAAGAAAGATGAAGAACAACTTCCTCAAGAGATAGTACTCAAAGTTGATATGCATTGTGAAGCTTGTGCAAGGAAAGTTGCAAAAGCATTGAAAGGATTTGAAGGAGTGGAGGAGGTGAGTGCAGATAGTAAGAGCAGCAAAGTGGGTAGTGAAAGGAAAGGCAGCAGACCCCATGAAAGTACTCAAAAGACTACAAAAGAAAAGTGGTAAGAAAGTGGAACTTATTTCACCTTTGCCTAAACCTCCACAAGACAAAAAAGAAGAAGAAGAGATCAAAGAACCACCAAAGCCAGAAGAGAAAAAAGATGAGGGTCCTACTGTGGTAACAATAGTTCTTAAAATAAGAATGCATTGTGATGCATGTGCTCAAGTTATACAGAAGAGAATCCGTAAGATAAAAGGAGTAGAATCAGTGGAAACAGATTTAGGGAACGACCAAGCCATAGTAAAAGGTGTGATTGATCCAGCAAAGCTTGTCGATGAAGTATACAAGAGAACCAAAAAGCAAGCTTCAATAGTGAAAGAGGAGGAAGAAAAGAAGGAAGAAGAGAAGAAAGAAGAGGAGAAAAGGAAGAGAAAAAGAAGGAGAAGAAGAAGAGAAAAAAGAGAGTGAGGAAGAAAACAAGGAAGAAGATGATAATAAAACTGAAATCAAGCGTAGTGAATATTGGCCATCAAAAGATTATATTGATTATGCTTATGCACCTGAGATTTTCAGTGATGAAAATCCAAATGCTTGTTCTGTTATGTAGACTTGTTGTTATTTGTAATGTTTCTGTATTTTAATAGCTTATTAGATTAAGTTAATTTTCTATTTCTATGTAACATAGTATAGTAGTGAATATTTTAAGGTTTTTAGCTTTCTGTTGTGTTTATGAGCTTTCAATGAATTTCACTGTCAAAAGATGGCAGAATCACTTATGTTTCTGTGTATGTGTTAAAGTGTACTGATACTGAATTTTGGGCTGCAATTTATTATRKTACCTATAATGTTGAACG
TARGET=808,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02759 Ps_contig_mira-and-tgicl-ass_franssen_24595
SEQUENCE=TCAACGCAGARTAVGBGCGGCGACGAATCATTTTTCTCTACGGCAATAGAGATCGGAAATTCGAACTTCTTTCTCTCTCTCTGAAAAATCGTAAAACCGTTTATCTTAGATCGGATCTATTCGACGTCGTTTCGGTAGGAATGGCTCCATTCAGCGGCGATGAAACGGCACCGTTTTTTGGCTTCCTTGGAGCCGCAGCTGCTCTCGTCTTCTCATGTATGGGAGCTGCGTATGGTACTGCAAAAAGTGGTGTGGGAGTGGCTTCAATGGGTGTGATGAGACCGGAGCTGGTGATGAAGTCGATTGTGCCGGTTGTTATGGCTGGTGTTTTGGGTATTTATGGTCTTATTATTGCTGTTATTATCAGTACAGGGATTAACCCAAAGGCCAAATCTTATTACCTTTTTGATGGTTATGCACATCTCTCCTCTGGTCTTGCTTGCGGCCTTGCCGGGCTCTCTGCTGGTATGGCTATTGGTATTGTTGGTGATGCTGGTGTTAGAGCCAATGCACAGCAGCCCAAACTTTTCGTTGGAATGATTCTTATCCTTATCTTTGCTGAAGCTCTTGCCCTATATGGTCTCATTGTCGGTATTATCTTGTCTTCACGTGCTGGCCAGTCTAGAGCCGAATAAGTAAGAAGTTGTGTTGCAAAATATTCTTTGACCACAAACCTTCTTATTAAGAGGGTGAGGGGTGAATTTTTAGTTGTTCGGTTAGTGATATGTTTGGACACCAATTGCAGTATTTAACAAATACTTGTGAAAGCAATAGCTCCACCTGCTATGTTATTTGATAGTGTTTGTATAAGTAGTCTGTCTTTGCTTAATAAAGCTTTTTAAGCTTGTCTCTTTTTTCCTTTTGAAATGTGTCATGTCGTATTATATGAGCCTACTTTGTTTTTGCTGCTTCTGAAACTAGTGCGTGTAATGGTTTAGTTGCGAATATAACTTGTATGCATTTGTGGAWTTTTTTTTTCATATTGGATGCAGGCTTAGTTCTAACTGCTCTAGAAACC
TARGET=72,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02760 Ps_contig_mira-and-tgicl-ass_franssen_24598
SEQUENCE=GAATGAATGGGTGAATAATAAAATAAAATAAAAAATATATAAGASAGKGAGACGGCAAGAGTCTGGTGGCTCTTTCTTCCTCCTTTGGACTTTGGTTGGTAGTGTAGTCACCGACTGCAGTGTCGTGGCTCACCCTCACCCTCTCCTCTTCTCTCCTCCTTCGACTCCGTAACTCTCTAACAAGATGGGAGAGAGGATCCCATCTGGGAACGGAAATGGGAATGGGAATGGAAGTTATTTTCAGTTCCCTCCTCCCGGTCCTTCTCCTATCAGGTCTTCCGATCATCGTGAAAGATTTTTGGCTGAACTATTGGCGGAGAGGCAGAAATTGGGACCCTTTTTGCAAGTTCTCCCACAATGTACCAGGCTCTTAACTCAAGAAATCAGACGCATATCCGGATTTAATCAAGGTTTTTCGGAACATGAAAGATTTGAGCCAGACAGTCCATTTAGGCCGTTAGGTCAACATCCAAATAGTAGGCCAATGGATATGGAGGGATGGCCTGCTGCACCTAGAGAGGACAATGGAAATCTCCAAAGAATGGGACCTTTTCAAGCACCACCAGTGGGTTGGCATGGGGCACAAGGAATTCCGACGACTCCTGTAGTGAAGAGAGTTATCAGGCTTGACGTTCCTGTCGACAAATATCCAAATCAGTATAATTTTGTTGGTCGGATTCTGGGACCTCGCGGTAACTCGTTAAAAAGAGTGGAAGCGATGACAGAATGTAGGGTTTACATCAGAGGCTGTGGCTCCGTGAAGGATTCTATTAAGGAAGAGAAGCTTAAAGATAAACCTGGGTATGAGCACCTTAACGAGCCATTGCACTTGTTGGTGGAGGCAGAGTTTCCAGAGGATATAATCAATTCACGCTTGGACCATGCTGTTGCAGTACTAGAGAATCTTTTGAAGCCTGTGGATGAATCACTTGATCACTATAAGAAGCAGCAATTGAGGGAGTTAGCTATGCTTAATGGTACTCTGAGGGAGGAAAGTCCCAGTATGAGCCCTAGCATGAGTCCGAGCATGTCACCTTTCAATAGTAATGGAATGAAACGTGCCAAGACAGGAAGATGACCCCAGTACGTTAACCATGGATC
TARGET=17,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02761 Ps_contig_mira-and-tgicl-ass_franssen_24600
SEQUENCE=CCAAAATACCCGACTACTATGGGAGGGAGGCATATTACTACTACATCAGATGGGAATATAGCCCACATAACTGGTGTGGATGCTAGGACATATGAACATTCTTCCATTTCACAACCAGAAGCTTTGAAACCGGAATCCTCTGAAACTGCTCAGGAAAATCAATATTCATTTCCTTCATCTTCACACGAGTTTACCTATGAAAATGCCCAACAACCAGATCTTACATATCCTCATTTGCAAGCAAGCTCACATATCCAGAACCTATCTCCTTTCTCCAGTGTAATGGCATATACAAATTCTTTGCCCAATGCATTGTTGGCTTCAACGGTTCAAACAGCAAGGGAGGATATCCCGTATTCACCCTTCCCTGTTACACAATCAATGCCTGCAAAATATAGTAACATGGCTTCATCAATTGGTGGTCCTAATATGAACATGTCAGAGGCTTTAAGGGCAAACAACATTTCGACGCCTCAAGCTCAACCCAATCAACAAGCTTTGCCTGGTGCTGGTATTGCCACTGGACCTTCACTTCCTCAACACCTTGCCGTGCATCCGTACTCGCAGCCTACTCTTCCTTTAGGGCATTTTGCTAATATGATTAGCTATCCATTCATGCCTCAGAGCTATACATACATGCCATCGGCATTTCAGCAGGCTTTTGCTGGGAATAGCACATACCACCAATCTCTGGCAGCAGTGCTTCCACAATATAAAAACAGCATTTCTGTCAGCAGCTTGCCTCAGTCTGCTGCTATTCCATCGGGATATGGTTTTGGAAGTTCTACCAGCATTCCTGGTGGAAATTATCCTTTGAATCCGTCAGGTGCCCCTACCAGTACAACCATTGGATATGACGATGTTATAAGCTCCCAGTATAAAGACAACAGTCATATGATTTCACTACAGCAGCAGAATGAGAACAATCCTATGTGGGTTCAAGGGCCTGGTTCACGAACAATGTCAGTTCCTCCCAGCACGTATTACAGCTTCCAGGGACAGAATCAACAACAACAACAACAAAGTGGATTTCGGCAAAGCCAGCAGCCATCACAGCATTTTGCTGCACCCCATGGATATCCTAATTACTACCATTCTCAGAGTGGAATTTCTATGGAACATCAGCAGCAGAACCCTAGGGATGCTTCCCTTGCTGGCTCTCAAAGCCAACCACCTAAGCAGTCCCAACAACAGTTATGGCAAAACAGTTACTAATCCTAATCACCTCCCGGTTTTTAAGGGGCTTGATGTGATGTCAGTTTGAGAAAATGACCATTCAATCCGAGGCGTTCCCCATTGGTCTAAATGTTCCATTGTTTGGTCTGAATAGACCTGATATGGCCGCTCGCCCCCATGTGACGGAAGTTGTATTCTACATAATTTATGTGCTTGTGATTAGTTTTAAACG
TARGET=1006,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3097
PRIMER_LEFT_PENALTY=0.022302
PRIMER_RIGHT_PENALTY=0.287386
PRIMER_LEFT_SEQUENCE=CAGCAGCAGAATGAGAACAATC
PRIMER_RIGHT_SEQUENCE=CTTAGGTGGTTGGCTTTGAGAG
PRIMER_LEFT=906,22
PRIMER_RIGHT=1178,22
PRIMER_LEFT_TM=60.022
PRIMER_RIGHT_TM=60.287
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=273
PRIMER_PAIR_PENALTY_1=0.3158
PRIMER_LEFT_1_PENALTY=0.037486
PRIMER_RIGHT_1_PENALTY=0.278329
PRIMER_LEFT_1_SEQUENCE=CAGCACGTATTACAGCTTCCAG
PRIMER_RIGHT_1_SEQUENCE=GTTCTGCTGCTGATGTTCCATA
PRIMER_LEFT_1=974,22
PRIMER_RIGHT_1=1133,22
PRIMER_LEFT_1_TM=59.963
PRIMER_RIGHT_1_TM=60.278
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.3249
PRIMER_LEFT_2_PENALTY=0.037486
PRIMER_RIGHT_2_PENALTY=0.287386
PRIMER_LEFT_2_SEQUENCE=CAGCACGTATTACAGCTTCCAG
PRIMER_RIGHT_2_SEQUENCE=CTTAGGTGGTTGGCTTTGAGAG
PRIMER_LEFT_2=974,22
PRIMER_RIGHT_2=1178,22
PRIMER_LEFT_2_TM=59.963
PRIMER_RIGHT_2_TM=60.287
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.3815
PRIMER_LEFT_3_PENALTY=0.037486
PRIMER_RIGHT_3_PENALTY=0.343977
PRIMER_LEFT_3_SEQUENCE=CAGCACGTATTACAGCTTCCAG
PRIMER_RIGHT_3_SEQUENCE=TCACATCAAGCCCCTTAAAAAC
PRIMER_LEFT_3=974,22
PRIMER_RIGHT_3=1252,22
PRIMER_LEFT_3_TM=59.963
PRIMER_RIGHT_3_TM=60.344
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.3878
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.278329
PRIMER_LEFT_4_SEQUENCE=ACAATCCTATGTGGGTTCAAGG
PRIMER_RIGHT_4_SEQUENCE=GTTCTGCTGCTGATGTTCCATA
PRIMER_LEFT_4=922,22
PRIMER_RIGHT_4=1133,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=60.278
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_02762 Ps_contig_mira-and-tgicl-ass_franssen_24603
SEQUENCE=TACAGGAAAGAAAAGTACACACCTAGCAACCCAATAGCAATACTGTAACACAACTTCGGAACCATTAGATTAGCTAGTTTCAAATAAAAGACTGGATTGCCAAAATAATACATGTCACGTAATATGACAGTAATTTTGGATGAAATATAGTTCTAATGAACAAATGCTGTATAATATAAGTATATGTCCGAATATCCTAGAGTCTTCACTCTTCAGCTGCATGAATAACAGGAATTGGCTATGCTGTGTGATACGTCCTTACTCCTTAGAGTGATCGCAGAAGCATTCTCCGGATAATCGGAATGGAAGTTTTCAATGCATCCAGTTGAGGCTGCTGCTTCTTCTTTGAATAATATATATACATGCATAATTCATAATTAAGCTTGTTAATAGCATTATTAAAAAGGCACAAATTTCCCCCGACTTATTTAAATTAGGAGCTTCCTTAGAATATCTAGATGCAGATTCGACAGCGTAATTTATATGGCATACTTTACTCCCTTGGACAGATTCACAACATGCAAAACATTGCCGAAAACATCATAATAGACAACTTTGGCATTCATTTGGTCAAGCTCAACTGACATAAATCCTTGGCCATCATAGTAAAATTTGACTCCATATCCCCCACTTTTATCAACGTCCCCTTTCCATGCCTTTGAACCTCCACCACTAGTTAAGAACTGTATTTGGCTGCTGGTGCTACTTATGTGTTCCAAGCAATGGTCGTGCCCATTCATGTACATATCAACATTGTTGGCCTCAAGGATTGGTAGGAGGTGTGTTAAGAGCTCTTTGGTGTCGCCATGGTGCCCTATGCTTCGTACAGGATGATGTCCCACAACAATCTTCCACTTGGCAGTAGAATCCCTTAATGCTGTTTCCAGATCCTTCAAGAGGTTTGATAAATACTTCTTCCTTGGAAGTACACCTCTCCAATCATACTTGTGATCCTTTGGCTTCAAAAAGTACTTGTCCACAAAAGGAGTTGTATCGACGAAGAAGAACTCAGCGATTTCAGTGTGAACAATAAAAGATCTTTGGCAAAACCATCTGTGATCAATATTCTGAAGAATTGGATTTAATTGAGCTTCAACATCCCCCCTGTAGTCATGGTTGCCTAAGACATTATACCATTGCTTTTGAAGGCTGTTGGCGGTGTAGATATCTGAGAAGGAATATTGAAATGCAGGGTCATTTATCCCAGTCAATCCATCATCATAGAAGTTATCTCCGGTTGATACCACAAAGTCAATGTTTAATTTGTCAGCAACTCTTCCCATCTGAAAAGAAACTTGAGATTGGTTGTAGGTTCCTTTCCTTCCCCAGTCTCCAATGACGAGAAAGCTAAGTGAGACATCTGCTTTAACAGAAGGGTGGTCAAAGCGTTGGAGCTCAGCTGATGCTACACTTACAGCAACAATAACAGCGATGAAAGCAGGAATCCATAGCCAGACGGGGCTTAGGCTAGTTGTTCCTCCTAAACCACCCATGTTATGTTGTGTGTGTGTGTGTGCAGTACTAATAACAGACACCAGAACACTAGTTATATATAGAGAGAGGTGAGTTGGATAACATGATATAAATTTTTGCAACTGATCTTGTATTTGTATTGTCGGATCCTAGTAGTAAATTATCCATATAAATGAAGAAAAACAATTATATCCCCGA
TARGET=1501,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.8806
PRIMER_LEFT_PENALTY=0.067994
PRIMER_RIGHT_PENALTY=2.812649
PRIMER_LEFT_SEQUENCE=GATGAAAGCAGGAATCCATAGC
PRIMER_RIGHT_SEQUENCE=TCATGTTATCCAACTCACCTCTC
PRIMER_LEFT=1430,22
PRIMER_RIGHT=1579,23
PRIMER_LEFT_TM=60.068
PRIMER_RIGHT_TM=58.187
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=150
PRIMER_PAIR_PENALTY_1=2.8980
PRIMER_LEFT_1_PENALTY=0.067994
PRIMER_RIGHT_1_PENALTY=2.829981
PRIMER_LEFT_1_SEQUENCE=GATGAAAGCAGGAATCCATAGC
PRIMER_RIGHT_1_SEQUENCE=CATGTTATCCAACTCACCTCTCTC
PRIMER_LEFT_1=1430,22
PRIMER_RIGHT_1=1578,24
PRIMER_LEFT_1_TM=60.068
PRIMER_RIGHT_1_TM=59.170
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.833
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=2.8980
PRIMER_LEFT_2_PENALTY=0.067994
PRIMER_RIGHT_2_PENALTY=2.829981
PRIMER_LEFT_2_SEQUENCE=GATGAAAGCAGGAATCCATAGC
PRIMER_RIGHT_2_SEQUENCE=TCATGTTATCCAACTCACCTCTCT
PRIMER_LEFT_2=1430,22
PRIMER_RIGHT_2=1579,24
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=59.170
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=2.9772
PRIMER_LEFT_3_PENALTY=0.164560
PRIMER_RIGHT_3_PENALTY=2.812649
PRIMER_LEFT_3_SEQUENCE=CTGCTTTAACAGAAGGGTGGTC
PRIMER_RIGHT_3_SEQUENCE=TCATGTTATCCAACTCACCTCTC
PRIMER_LEFT_3=1360,22
PRIMER_RIGHT_3=1579,23
PRIMER_LEFT_3_TM=60.165
PRIMER_RIGHT_3_TM=58.187
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=2.9945
PRIMER_LEFT_4_PENALTY=0.164560
PRIMER_RIGHT_4_PENALTY=2.829981
PRIMER_LEFT_4_SEQUENCE=CTGCTTTAACAGAAGGGTGGTC
PRIMER_RIGHT_4_SEQUENCE=CATGTTATCCAACTCACCTCTCTC
PRIMER_LEFT_4=1360,22
PRIMER_RIGHT_4=1578,24
PRIMER_LEFT_4_TM=60.165
PRIMER_RIGHT_4_TM=59.170
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.833
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_02763 Ps_contig_mira-and-tgicl-ass_franssen_24609
SEQUENCE=AGCTTATCACAAGTGAAGGGTTGAGTTCTTTTAGTTCTTTGACAACAAGGGATGAGAATCTCTTTTTGTTCTGTGTCAGTTTTGTTGATCTTGTTAAGGGTTTGAAGCCTTGGAAACATAGGTTTGATGGTTTGAGGTTGTGTGTTTGGTGTTTTTGGAGTGTGGATGTTAGGGCGTTTGTTGAGATCATGGATGATGCTGATGCTGCCATTGTTTTTGGTTTGATGAGTGAATGGAATGGAATGGAATCCCGCGTACT
TARGET=231,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02764 Ps_contig_mira-and-tgicl-ass_franssen_24610
SEQUENCE=AGTTCTTTGATCCTCTTGGTTTGGCCAATGATCCTGAAGAGAAAGAAAGACTTCAACTGGCAGAGATTAAGCATTCTCGCTTGGCCATGGTGGTTTTCCTTGTCTTTGCAATTCAAGCTGCTGTTTCTGGAAAAGGACCTATTGGTTTCATTGCTACCTTCAACCAGTGACATAATTATATATGAAGTGAAACCCTTTTAGCTCAAAATCTTCTCTATCATGTATAAGTCTAAATATCACTCACTCCATGTTAAAATTGTTTATATTTTTATATGTATCCGCAGAAATTAAGAACTGCTATGTATGTATGTATGTACTTCTGTGTTGTATGCTTCTCGGTAATACCATACTATAATCCTGATCCATATAATATAGTGTTGCTTATAGCAGCAATGTAATACAAAGTGTTATTTTTYCTATTCTATAGAGAAACATTCACTGACCTTTGCATT
TARGET=299,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02765 Ps_contig_mira-and-tgicl-ass_franssen_24622
SEQUENCE=AGGGTGGACGTGGGGGTTATGGAGGTGGACGCGGTGGTCGTSGGRTACARCCATGGAGGCGGTGGTTACAACGGTGGCGGTGGAGGATACAACCATGGTGGCGGAGGAGGATACAACCATGGAGGCGGTGGTTACAATGGTGGTGGAGGAGGCTACAACCATGGAGGTGGTGGTTATCGTGGTGGAGGTGGACATGGTGGTAGTTTCAACAATGGTAACTGATTATATCATCATGCATGCATGTGTTAAGTGTAATAAATAATGTTGGGTTTTCAACAAAAGAGTAAAGATAAATCACTATGTATATGTGAAGATGAGTTTGATTTCGAGTAAAAGTGGTGAATGTGTTATCATGTGTAATATATCAAATATCATCGTTGTTGTTRAAAGT
TARGET=232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02766 Ps_contig_mira-and-tgicl-ass_franssen_24635
SEQUENCE=ATGTTGATATTAAGGGAAATGATTTTGAAGTTATACCTTTTGGTGCTGGACGGAGAATTTGTGTTGGGATGAGTCTTGGACTTCGTATGGTTCAACTTTTAACTGCTACGTTAGCCCATTCGTTTAATTGGGAACTTGAGAGTGGGCTTAAAGCTGAAAAGATGAACATGGATGAAGCTTTTGGACTTACTCTTCAACGAGCCGTGCCATTATCTGTGTATCCTAAACCTAGGCTCTCTCCTAATGTGTATTAATCATGTTTCTAATTTTACATATTTATTTTTGACAATTTTTCTTTTTCCGAGAGATCCTTCTGATTWCTTTATCTTATCTTTAAATATAATAATGTAAATAAATAAAATTGAGTATGTTGTGTGGAGGGGATTACTAAAAAGGATCGGAGGGTGTAACGTCTATTAATTTATGGTTTGTATTTACTAAAAA
TARGET=349,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02767 Ps_contig_mira-and-tgicl-ass_franssen_24640
SEQUENCE=CAACTGTGAGTGTCTCCTCAATGAGGTCGTCCACCGAGACAAGGTCATCGATAATTGTCAACATGATTTGTAGCTTCTTGATTCCGTATCCGACAGCAACAAGCTTGGATGCTCCCCAGGTTAAACCGGGAATCTCAACGCTGCGAACAGCCTCTTCCAGCTTCTTCATGTCTGTCTCATCATCCCAAGGCTTAACATCAAGCAGAATGGAAGACTTTCCGCTTTCTTTCTTCTTGGCAGGTTTTTTAGCAGCCTCCCTTTCCTCTGCTGCCTTCTTCTCATCCTCAGTTTCATCACCGAAGAGATCAAGATCATCATCATCTTCAGCGGCGGGAGCATCTGCTTTGGCAGGTGCTTCTGCAGCTGGAGCAGCACCACTTCCTGTAAACTTCACACCTTGAGCTTTTCCAGGGAAACTAGCAGCAAGACTTGAAGACACAGCATCATACCAATTAGCAGCATTGGGGAAAGACTCACTTGGCTTCTCAGTAACAGCTCCATACACTTTGATATCATCCTTCGTCAATTGATCCCCAGAGATGTAGGTTTTTCCGGAGAGGAATTCATCGAGGGATTTGATACCCTGTTCTGTGTGTAGATCTGAGAAAGTAACGGCCATTGGAGTTTGGGATGAATCGGTGGCGGAGAGTGAAGAGAAGAGAGTGTGTGAAGAAGTGTGGAGAGTTGAGAGGCTCCCTGCAGTGGCGGCAGTGGAAGCTATTAACAAGATAGTGACAGGGAGTCTTGTGAGTCT
TARGET=311,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0911
PRIMER_LEFT_PENALTY=0.016312
PRIMER_RIGHT_PENALTY=0.074807
PRIMER_LEFT_SEQUENCE=CTGCCTTCTTCTCATCCTCAGT
PRIMER_RIGHT_SEQUENCE=AGTCTTGCTGCTAGTTTCCCTG
PRIMER_LEFT=267,22
PRIMER_RIGHT=429,22
PRIMER_LEFT_TM=60.016
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.1030
PRIMER_LEFT_1_PENALTY=0.016312
PRIMER_RIGHT_1_PENALTY=0.086698
PRIMER_LEFT_1_SEQUENCE=CTGCCTTCTTCTCATCCTCAGT
PRIMER_RIGHT_1_SEQUENCE=ATGGAGCTGTTACTGAGAAGCC
PRIMER_LEFT_1=267,22
PRIMER_RIGHT_1=500,22
PRIMER_LEFT_1_TM=60.016
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.1411
PRIMER_LEFT_2_PENALTY=0.066262
PRIMER_RIGHT_2_PENALTY=0.074807
PRIMER_LEFT_2_SEQUENCE=TTCATGTCTGTCTCATCATCCC
PRIMER_RIGHT_2_SEQUENCE=AGTCTTGCTGCTAGTTTCCCTG
PRIMER_LEFT_2=164,22
PRIMER_RIGHT_2=429,22
PRIMER_LEFT_2_TM=59.934
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.1697
PRIMER_LEFT_3_PENALTY=0.016312
PRIMER_RIGHT_3_PENALTY=0.153359
PRIMER_LEFT_3_SEQUENCE=CTGCCTTCTTCTCATCCTCAGT
PRIMER_RIGHT_3_SEQUENCE=ATTCCTCTCCGGAAAAACCTAC
PRIMER_LEFT_3=267,22
PRIMER_RIGHT_3=562,22
PRIMER_LEFT_3_TM=60.016
PRIMER_RIGHT_3_TM=59.847
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.2014
PRIMER_LEFT_4_PENALTY=0.186600
PRIMER_RIGHT_4_PENALTY=0.014792
PRIMER_LEFT_4_SEQUENCE=TTCATCACCGAAGAGATCAAGA
PRIMER_RIGHT_4_SEQUENCE=CCAATGCTGCTAATTGGTATGA
PRIMER_LEFT_4=289,22
PRIMER_RIGHT_4=464,22
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=59.985
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=176
=
PRIMER_SEQUENCE_ID=SSR_02768 Ps_contig_mira-and-tgicl-ass_franssen_24643
SEQUENCE=GGAAGATAGTTAATTAGTAGTTAGTTAGTTAATTATCAATCTGATTCTTATATTGGTTTTAATCTTTTGGTTATCTTGATTCTGTGCAGAAAAATGCTAAAGTTGTTTCAATTGATGACTATGAGGATGTTCCCGCTTATGATGAAGGTGCTTTGAAAAAGGCTGTTGCAAATCACCGTGTGACTAAAAGAGCTTGGAAGGTGTGTTATAAAGTCCATGTCCTGGCCAAGAAAAGGAARTTGCTAATCGTGCAACAATAGGTTTAACACCAGAGTGACCAGCTGTGGGGTCTTGTGGTACTCATGAGGAGGTACTGTCAAACTTGATAACACCAGATATCTGCA
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02769 Ps_contig_mira-and-tgicl-ass_franssen_24673
SEQUENCE=CACCTAAAGAAACTGAGGAATCGGTTGAAGAAGTGAAGGAAGAGGCGGAAGCAGTAGTGGAGGAGCCAATAGCAACTACTGAAAATGAGTCAGCACCACCACCACCAGCAACTGCAGAGGATGTGATTGAGAACAAAACAGTTGAATCAGTGGAAGCTCCAGCAGCAGAGGATTCCAGTTGAGAATCCTGAAGCTTAAACATGGAATGGGTGTGACTGGAACTAAGGTAAAAAACGGTAATTTAAATTAATGGTT
TARGET=94,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1168
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_RIGHT_SEQUENCE=AAGCTTCAGGATTCTCAACTGG
PRIMER_LEFT=24,22
PRIMER_RIGHT=195,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.2240
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.114470
PRIMER_LEFT_1_SEQUENCE=ATCGGTTGAAGAAGTGAAGGAA
PRIMER_RIGHT_1_SEQUENCE=AAGCTTCAGGATTCTCAACTGG
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=195,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.2240
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=AATCGGTTGAAGAAGTGAAGGA
PRIMER_RIGHT_2_SEQUENCE=AAGCTTCAGGATTCTCAACTGG
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=195,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=0.2240
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=GAAACTGAGGAATCGGTTGAAG
PRIMER_RIGHT_3_SEQUENCE=AAGCTTCAGGATTCTCAACTGG
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=0.2240
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.114470
PRIMER_LEFT_4_SEQUENCE=AGAAACTGAGGAATCGGTTGAA
PRIMER_RIGHT_4_SEQUENCE=AAGCTTCAGGATTCTCAACTGG
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=195,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_02770 Ps_contig_mira-and-tgicl-ass_franssen_24676
SEQUENCE=TGTTTTGGTTAAGTTTGTTAAAGGTATTTGGTTGTTTTGTCCATTCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTTACTGTGTACACTT
TARGET=171,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02771 Ps_contig_mira-and-tgicl-ass_franssen_24709
SEQUENCE=AAAAATTAGGAGACCGGAATACCACGTGAATACCAAAGCTTATACAGATTATGAATGGATGAAATTTTAACCAATTACGGTAACATTCAGCTACAGACATAGCCATAAATACTGAGACTGCCTAAACTACCAACAAACCTATCAAAAATGTCTTTTACCAGTTTTAGCTGTGTCTCACAATAAAAAAAACATAAGTAAAAACAAAACTTCAATGGGAAACAAAAAATAAGTTTGTAACATTCCATGTTCCTTTCCAACTCCCTTGACAATCTACTTCAGTTTTCTAGTTTTCTTCAACGTATGATGATGCTCTTTAGTTTTCTTCAACGTGTTCTTCCTCCACTTTCTCGGCACTTTCTGAACGACCATCATCAGATGGTGAAGATTTCCTCCAACTCCTCTCAGATCGATCTTCCGCACGTCCATTGCCAGAACCAAAACCCCTCTTTCCGAACCCTTCCACTGCTGTCTCCTTAATCTTCATGCTCTCAAGTTGTTCATCAATCTTCTTCCAATCCTGACCTTTCTCTGCCAAAACCTGCTCCCTTGGCCTAGCTTCACCAAATGGATTTGCACCCTTAGGCTTAGCCACAATCCCAGAAACAGCACCAGCAGCAGGAACATCCTGGTTTTCATTACTCGCAGATGGAGACCGCGGTTGCAAAACAAGCCTCGGCCTTCCACCAGCAACCGTCTCGGTTCTCTCACTTCCAACACTAAATTCACCTTTTTTCCTATTCCAATTATCCGAATCCGCACCACCGCTGGTACCAAACCCTACTTTCCTCTCTCTATCAAATCCTCCACCACCACCACTGAATCTCCCGTCCCTCCGACGGCACAWAACTCTTACTCGAAACCCAACTATCAGATTCATCAGCACGCGACTGCGAATCAAAAAACCCACCTCTCTCCCCTCTATCCCTATCCCTTCTCTCAAATCCATTCCCAACCGCAGGCTTCTTTGCAGCCGCCCAATTATCAATCTCATCCGCCCTAGACGGTGCAGGTGCAGTCTCCCGATTCGAATCCCTGGAACCAAAGGAATCATTCCGCCTCAATTCACTAGAAACCCTAGAGGAACCCCATCTACGAAACAGAACTCTCATCTCCACCACG
TARGET=804,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02772 Ps_contig_mira-and-tgicl-ass_franssen_24713
SEQUENCE=ATYAAACAAAACAAAAATCAAATGCATTCTATGATTTTTGAGCAGAGTCCCCAGTGTCTCTAAAGCTTAAGAAAATGATTATCATACCGGAAACTATTTGAAGAACGAACACAATGAAATGACAAATTAACTTTAGTAAATAAAAAACAATCTATTGACAAACAGGTACAAAGAGTAGTAAATAGAAATTATTGCTGGACATCATAGAAAGAAAGAARRAAGAATACAGTGGACG
TARGET=206,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02773 Ps_contig_mira-and-tgicl-ass_franssen_24716
SEQUENCE=GTTCGGGGGTTCCTATAACCGTTCCTATAGGTTAAAAGATATATTTGGGCCTGTGTAATATAAAAGGCTAATTTGGGAATTATACAARTYCATGAGATAAAACGATAAGGGATAAGAGTCTGAGAAAGGAGAAACCGCATAGGAGAGAAAAGAGATAGAAAAATGGCGTCAGTGTCGAGCATATTTGGGTGTGGTGTATCGATGGCACCAAATTCTTCACTGAGAAACAAAGCAATTCGAACTGAAAGAAGAAGTGCGTGTGGAGGATTGTTGATTGAGTGTTCATCAAGGCCACAGAAGAAATCAACTGCACATCACATGAAGACGAGGCCGAGAAAATCACGACTGTCCGATAGGAATCGGAAGCCGACTGTGTATGCTCCGTTGCCTCCGCTACCTCCTGATTTTACGATTGTGATTCCTGCTGATGCATCGACGGTTGATTTTACTCCTCCGCCTCCCACTCCTTCTGATTGATTCGGTACTTTTTTTGTATGTTGATGTGTTGTTGTTGTTCTCTTTTGTTGGATTTTGAATTGTATTGTTAAGATATACTATTGTATTATTTCAGTTTTGTTTGTCAATTAACTACTGTGCTTTTTTATGTTTATATATGATGATGATGATAAAATGGCGATGTTTTT
TARGET=504,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02774 Ps_contig_mira-and-tgicl-ass_franssen_24716
SEQUENCE=GTTCGGGGGTTCCTATAACCGTTCCTATAGGTTAAAAGATATATTTGGGCCTGTGTAATATAAAAGGCTAATTTGGGAATTATACAARTYCATGAGATAAAACGATAAGGGATAAGAGTCTGAGAAAGGAGAAACCGCATAGGAGAGAAAAGAGATAGAAAAATGGCGTCAGTGTCGAGCATATTTGGGTGTGGTGTATCGATGGCACCAAATTCTTCACTGAGAAACAAAGCAATTCGAACTGAAAGAAGAAGTGCGTGTGGAGGATTGTTGATTGAGTGTTCATCAAGGCCACAGAAGAAATCAACTGCACATCACATGAAGACGAGGCCGAGAAAATCACGACTGTCCGATAGGAATCGGAAGCCGACTGTGTATGCTCCGTTGCCTCCGCTACCTCCTGATTTTACGATTGTGATTCCTGCTGATGCATCGACGGTTGATTTTACTCCTCCGCCTCCCACTCCTTCTGATTGATTCGGTACTTTTTTTGTATGTTGATGTGTTGTTGTTGTTCTCTTTTGTTGGATTTTGAATTGTATTGTTAAGATATACTATTGTATTATTTCAGTTTTGTTTGTCAATTAACTACTGTGCTTTTTTATGTTTATATATGATGATGATGATAAAATGGCGATGTTTTT
TARGET=614,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02775 Ps_contig_mira-and-tgicl-ass_franssen_24728
SEQUENCE=TGGCGTCATTTACTTCATTTGACTTTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATCGACCGTACGCCTCCATCGACCGTACGCCCTCCATCGTCCATCGACCATACGCCA
TARGET=89,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9186
PRIMER_LEFT_PENALTY=0.621889
PRIMER_RIGHT_PENALTY=2.296722
PRIMER_LEFT_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_SEQUENCE=GTGGTGGAGGTTACGGTCAT
PRIMER_LEFT=27,22
PRIMER_RIGHT=146,20
PRIMER_LEFT_TM=59.378
PRIMER_RIGHT_TM=59.703
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=2.9186
PRIMER_LEFT_1_PENALTY=0.621889
PRIMER_RIGHT_1_PENALTY=2.296722
PRIMER_LEFT_1_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_1_SEQUENCE=GTGGTGGAGGTTACGGTCAT
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=167,20
PRIMER_LEFT_1_TM=59.378
PRIMER_RIGHT_1_TM=59.703
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=2.9186
PRIMER_LEFT_2_PENALTY=0.621889
PRIMER_RIGHT_2_PENALTY=2.296722
PRIMER_LEFT_2_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_2_SEQUENCE=GTGGAGGTTACGGTCATGGT
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=164,20
PRIMER_LEFT_2_TM=59.378
PRIMER_RIGHT_2_TM=59.703
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=138
PRIMER_PAIR_PENALTY_3=2.9186
PRIMER_LEFT_3_PENALTY=0.621889
PRIMER_RIGHT_3_PENALTY=2.296722
PRIMER_LEFT_3_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_3_SEQUENCE=GAGGTTACGGTCATGGTGGT
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=161,20
PRIMER_LEFT_3_TM=59.378
PRIMER_RIGHT_3_TM=59.703
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=3.0735
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=2.451602
PRIMER_LEFT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=GGTCATGGTGGTGGAGGTTAC
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=153,21
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=61.452
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=127
=
PRIMER_SEQUENCE_ID=SSR_02776 Ps_contig_mira-and-tgicl-ass_franssen_24728
SEQUENCE=TGGCGTCATTTACTTCATTTGACTTTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATCGACCGTACGCCTCCATCGACCGTACGCCCTCCATCGTCCATCGACCATACGCCA
TARGET=113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9186
PRIMER_LEFT_PENALTY=0.621889
PRIMER_RIGHT_PENALTY=2.296722
PRIMER_LEFT_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_SEQUENCE=GTGGTGGAGGTTACGGTCAT
PRIMER_LEFT=27,22
PRIMER_RIGHT=146,20
PRIMER_LEFT_TM=59.378
PRIMER_RIGHT_TM=59.703
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=2.9186
PRIMER_LEFT_1_PENALTY=0.621889
PRIMER_RIGHT_1_PENALTY=2.296722
PRIMER_LEFT_1_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_1_SEQUENCE=GTGGTGGAGGTTACGGTCAT
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=167,20
PRIMER_LEFT_1_TM=59.378
PRIMER_RIGHT_1_TM=59.703
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=2.9186
PRIMER_LEFT_2_PENALTY=0.621889
PRIMER_RIGHT_2_PENALTY=2.296722
PRIMER_LEFT_2_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_2_SEQUENCE=GTGGAGGTTACGGTCATGGT
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=164,20
PRIMER_LEFT_2_TM=59.378
PRIMER_RIGHT_2_TM=59.703
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=138
PRIMER_PAIR_PENALTY_3=2.9186
PRIMER_LEFT_3_PENALTY=0.621889
PRIMER_RIGHT_3_PENALTY=2.296722
PRIMER_LEFT_3_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_3_SEQUENCE=GAGGTTACGGTCATGGTGGT
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=161,20
PRIMER_LEFT_3_TM=59.378
PRIMER_RIGHT_3_TM=59.703
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=3.0735
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=2.451602
PRIMER_LEFT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=GGTCATGGTGGTGGAGGTTAC
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=153,21
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=61.452
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=127
=
PRIMER_SEQUENCE_ID=SSR_02777 Ps_contig_mira-and-tgicl-ass_franssen_24756
SEQUENCE=GATCCTTCATTACCAAATATCACATTTCAATTGATCTTAGAATTGAGTACAATGGATTCCAAATCATCATTCCTCATCCTTGGCCTAATGGCCATGATTCTTCTTATTTCCTCAGAGGTGGCAGCTAGGGACTTAACTGAGACTTCCACTAATACCAAAGAGGGAGAAGACAACGGACAGGAACCAATTTCCTATGGTGGTTATGGAGGCTACGGTCATGGCGGTGGTTACAATAATGGTGGCGGTTATCATAATGGTGGAGGGGGTTACAATAATGGTGGTGGATACCATAACGGTGGTGGTGGTTACAACAACGGTGGTGGTTACCATAATGGCGGAGGTGGTTACAACAACGGTGGAGGTTATCATAATGGTGGTGGTGGCTACAATGGTGGTGGTTACCATAACGGCGGAGGTGGTTACAATGGTGGAGGTGGTTACCATGGTGGTTACAACAATGGTAACTGATTATATCAATGTTCTTATTAAGAAAATTTCATGAATAAATAATGGTAAGTGTTGAACAAAAGAGGTATATTAATCTATCTTCCCACAATTTAGTATAGTTATTTCCCAACTCTGTAAATCACTATGTATATGTGATGATGAGTTTGATTTCTGACAAAAGTGGTGAATGTTTTGTCATTTGTAATATATCACATATCATCGTTGTTAGTAATAAAAAGGCATGTATTAATC
TARGET=295,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1024
PRIMER_LEFT_PENALTY=0.068302
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=TTTCCTATGGTGGTTATGGAGG
PRIMER_RIGHT_SEQUENCE=ACCATTATGATAACCTCCACCG
PRIMER_LEFT=187,22
PRIMER_RIGHT=374,22
PRIMER_LEFT_TM=60.068
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.1024
PRIMER_LEFT_1_PENALTY=0.068302
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=TTTCCTATGGTGGTTATGGAGG
PRIMER_RIGHT_1_SEQUENCE=CCATTATGATAACCTCCACCGT
PRIMER_LEFT_1=187,22
PRIMER_RIGHT_1=373,22
PRIMER_LEFT_1_TM=60.068
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.2509
PRIMER_LEFT_2_PENALTY=0.216800
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=CCTATGGTGGTTATGGAGGCTA
PRIMER_RIGHT_2_SEQUENCE=CCATTATGATAACCTCCACCGT
PRIMER_LEFT_2=190,22
PRIMER_RIGHT_2=373,22
PRIMER_LEFT_2_TM=60.217
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=0.3088
PRIMER_LEFT_3_PENALTY=0.274705
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=TTCTTATTTCCTCAGAGGTGGC
PRIMER_RIGHT_3_SEQUENCE=ACCATTATGATAACCTCCACCG
PRIMER_LEFT_3=100,22
PRIMER_RIGHT_3=374,22
PRIMER_LEFT_3_TM=59.725
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=0.3088
PRIMER_LEFT_4_PENALTY=0.274705
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=TTCTTATTTCCTCAGAGGTGGC
PRIMER_RIGHT_4_SEQUENCE=CCATTATGATAACCTCCACCGT
PRIMER_LEFT_4=100,22
PRIMER_RIGHT_4=373,22
PRIMER_LEFT_4_TM=59.725
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_02778 Ps_contig_mira-and-tgicl-ass_franssen_24756
SEQUENCE=GATCCTTCATTACCAAATATCACATTTCAATTGATCTTAGAATTGAGTACAATGGATTCCAAATCATCATTCCTCATCCTTGGCCTAATGGCCATGATTCTTCTTATTTCCTCAGAGGTGGCAGCTAGGGACTTAACTGAGACTTCCACTAATACCAAAGAGGGAGAAGACAACGGACAGGAACCAATTTCCTATGGTGGTTATGGAGGCTACGGTCATGGCGGTGGTTACAATAATGGTGGCGGTTATCATAATGGTGGAGGGGGTTACAATAATGGTGGTGGATACCATAACGGTGGTGGTGGTTACAACAACGGTGGTGGTTACCATAATGGCGGAGGTGGTTACAACAACGGTGGAGGTTATCATAATGGTGGTGGTGGCTACAATGGTGGTGGTTACCATAACGGCGGAGGTGGTTACAATGGTGGAGGTGGTTACCATGGTGGTTACAACAATGGTAACTGATTATATCAATGTTCTTATTAAGAAAATTTCATGAATAAATAATGGTAAGTGTTGAACAAAAGAGGTATATTAATCTATCTTCCCACAATTTAGTATAGTTATTTCCCAACTCTGTAAATCACTATGTATATGTGATGATGAGTTTGATTTCTGACAAAAGTGGTGAATGTTTTGTCATTTGTAATATATCACATATCATCGTTGTTAGTAATAAAAAGGCATGTATTAATC
TARGET=372,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3892
PRIMER_LEFT_PENALTY=0.296177
PRIMER_RIGHT_PENALTY=0.093026
PRIMER_LEFT_SEQUENCE=GACAGGAACCAATTTCCTATGG
PRIMER_RIGHT_SEQUENCE=GTAACCACCATGGTAACCACCT
PRIMER_LEFT=175,22
PRIMER_RIGHT=452,22
PRIMER_LEFT_TM=59.704
PRIMER_RIGHT_TM=59.907
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=278
PRIMER_PAIR_PENALTY_1=0.3892
PRIMER_LEFT_1_PENALTY=0.296177
PRIMER_RIGHT_1_PENALTY=0.093026
PRIMER_LEFT_1_SEQUENCE=GGACAGGAACCAATTTCCTATG
PRIMER_RIGHT_1_SEQUENCE=GTAACCACCATGGTAACCACCT
PRIMER_LEFT_1=174,22
PRIMER_RIGHT_1=452,22
PRIMER_LEFT_1_TM=59.704
PRIMER_RIGHT_1_TM=59.907
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.6770
PRIMER_LEFT_2_PENALTY=0.583983
PRIMER_RIGHT_2_PENALTY=0.093026
PRIMER_LEFT_2_SEQUENCE=CAGGAACCAATTTCCTATGGTG
PRIMER_RIGHT_2_SEQUENCE=GTAACCACCATGGTAACCACCT
PRIMER_LEFT_2=177,22
PRIMER_RIGHT_2=452,22
PRIMER_LEFT_2_TM=60.584
PRIMER_RIGHT_2_TM=59.907
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=1.0465
PRIMER_LEFT_3_PENALTY=0.296177
PRIMER_RIGHT_3_PENALTY=0.750366
PRIMER_LEFT_3_SEQUENCE=GACAGGAACCAATTTCCTATGG
PRIMER_RIGHT_3_SEQUENCE=CGTTATGGTAACCACCACCATT
PRIMER_LEFT_3=175,22
PRIMER_RIGHT_3=408,22
PRIMER_LEFT_3_TM=59.704
PRIMER_RIGHT_3_TM=60.750
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=1.0465
PRIMER_LEFT_4_PENALTY=0.296177
PRIMER_RIGHT_4_PENALTY=0.750366
PRIMER_LEFT_4_SEQUENCE=GGACAGGAACCAATTTCCTATG
PRIMER_RIGHT_4_SEQUENCE=CGTTATGGTAACCACCACCATT
PRIMER_LEFT_4=174,22
PRIMER_RIGHT_4=408,22
PRIMER_LEFT_4_TM=59.704
PRIMER_RIGHT_4_TM=60.750
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_02779 Ps_contig_mira-and-tgicl-ass_franssen_24765
SEQUENCE=TTTTTTTAAAATTAATTGTTGGAAAAAAATAGAGCTAGAAATTGAAGCTTAACTGCTTTAGAAAATAAGCCATTTGAAGGAACTAAAATCAACTAAGACGATAGATGTTAATAAGTCATGTCTTGTGGACACTTATTGAAGTGCTATCCAAACGCCATTATTCAAATGCTGTTCAAATTATTATAAACCACAGCTTGTGACAGTAGAGTTTGAATGTGAATGACTTTTTAATTAAGCAATTTATGCATTTCAATCAAATGTCATTTGTTCAGCTTCATGCCGGATTGATTCGAAAAGTGCTGAAGACAGATAACGTTCTCCAAAACTGGGAAAAATCGCAACGATAAGCTTTCCTGCATTTTCTGGTCTCTTTCCTAGTTTTATTGCAGCTGCAGCAGCAGCACCGGATGAAATTCCCATCAGTAAACCTTCTTTCAAAGCAAGCAACTTAGCAGTTTCTATAGCATCTTCACTTGAAACCTGAATAACTTCATCTAGAAAATCAAATTCCAGAATAGGGGGAACGATACCGGCACCGAGCCCTTGGATTAGATGTTTGCCTGGCTTTCCTCCATTCAGAACCGCACTTTCAGCTGGTTCCACACCATACACCTTGATTTCTGAGTTTTTCTCCTTGAGGAACTTCCCTGTACCTGTTACAGTTCCCCCGGTTCCTATCCCTGCAACCAAAGCATCAATTTTCCCTCCAGAGTCTCTCCAAATCTCAGGGCCAGTTGTCTCATAATGAATCTTTGCATTGGCAGGGTTTTCAAATTGATTGAGCTTATAACTATTAGGCGTCTTCGCTAATAGCTCTTCCGCTTTCTCAAAAACACCATCAATTCCTTTGGCAGGATCTGTCAAATACACCTCAGCTCCAAAAGCTAGCAGTATAATTTTTCTCTCAAGACTCACATAAGATGGCATAGTCACTAAAAGCTTATAGCCTCTCAGCGCTGCTATAGACGCCAATCCTATGCCGGTGTTACCACTTGTAGGCTCAACAAGGACAGTCTTTCCAGGTGTAATGAGGCCTTTGTTTTCAGCATCTTCAATCATGCTTAATGATATTCTATCTTTAACACTGCAGCAGGATTGCAAGTATTCTAGCTTAGCAGCGATTCGAGCAACACAGCCTTCAGTAATATTGTTAAGATATACCAATGGAGTATTCCCTATTAATTCAGTGGCATCTTTCTTGATTGCAAACTGTGTCTCCATGATATCTGATGAAGAAAGCCTGCAGAAGCTTGATAGTTGAGTCTCTTTTAGAAACTAGCAGAAAAAGAAAGCAAACATCTCAATAATTGCTGGGCACCATAAAACTCTAAAAATGAGTAAACAAAGGAGATTGAAAATGTTGTACTAACCAATCCYYCAATGACC
TARGET=392,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02780 Ps_contig_mira-and-tgicl-ass_franssen_24766
SEQUENCE=CGGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGTTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCTCCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTATCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCAGTTGTTTGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTAATAAAGT
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1821
PRIMER_LEFT_PENALTY=0.154023
PRIMER_RIGHT_PENALTY=0.028056
PRIMER_LEFT_SEQUENCE=TCTCTCACTCTTGCATTAGCCA
PRIMER_RIGHT_SEQUENCE=TGAGGATAGGTGTGAACTGGTG
PRIMER_LEFT=58,22
PRIMER_RIGHT=224,22
PRIMER_LEFT_TM=60.154
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.1887
PRIMER_LEFT_1_PENALTY=0.160609
PRIMER_RIGHT_1_PENALTY=0.028056
PRIMER_LEFT_1_SEQUENCE=ATGGCTTCTGCTTCTCTCACTC
PRIMER_RIGHT_1_SEQUENCE=TGAGGATAGGTGTGAACTGGTG
PRIMER_LEFT_1=46,22
PRIMER_RIGHT_1=224,22
PRIMER_LEFT_1_TM=60.161
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=179
PRIMER_PAIR_PENALTY_2=0.3238
PRIMER_LEFT_2_PENALTY=0.154023
PRIMER_RIGHT_2_PENALTY=0.169745
PRIMER_LEFT_2_SEQUENCE=TCTCTCACTCTTGCATTAGCCA
PRIMER_RIGHT_2_SEQUENCE=GAGGAGAGTGGTAGACTGGGTG
PRIMER_LEFT_2=58,22
PRIMER_RIGHT_2=301,22
PRIMER_LEFT_2_TM=60.154
PRIMER_RIGHT_2_TM=60.170
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=59.091
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.3304
PRIMER_LEFT_3_PENALTY=0.160609
PRIMER_RIGHT_3_PENALTY=0.169745
PRIMER_LEFT_3_SEQUENCE=ATGGCTTCTGCTTCTCTCACTC
PRIMER_RIGHT_3_SEQUENCE=GAGGAGAGTGGTAGACTGGGTG
PRIMER_LEFT_3=46,22
PRIMER_RIGHT_3=301,22
PRIMER_LEFT_3_TM=60.161
PRIMER_RIGHT_3_TM=60.170
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=59.091
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.3569
PRIMER_LEFT_4_PENALTY=0.328860
PRIMER_RIGHT_4_PENALTY=0.028056
PRIMER_LEFT_4_SEQUENCE=AATGAGGTCCCTAATGGCTTCT
PRIMER_RIGHT_4_SEQUENCE=TGAGGATAGGTGTGAACTGGTG
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=224,22
PRIMER_LEFT_4_TM=60.329
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_02781 Ps_contig_mira-and-tgicl-ass_franssen_24766
SEQUENCE=CGGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGTTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCTCCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTATCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCAGTTGTTTGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTAATAAAGT
TARGET=347,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4623
PRIMER_LEFT_PENALTY=0.215550
PRIMER_RIGHT_PENALTY=0.246726
PRIMER_LEFT_SEQUENCE=ACCTCCACCAGTTCACACCTAT
PRIMER_RIGHT_SEQUENCE=AATGGTAGACAGGGTGAGGATG
PRIMER_LEFT=198,22
PRIMER_RIGHT=394,22
PRIMER_LEFT_TM=59.784
PRIMER_RIGHT_TM=60.247
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=0.6300
PRIMER_LEFT_1_PENALTY=0.203343
PRIMER_RIGHT_1_PENALTY=0.426682
PRIMER_LEFT_1_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_RIGHT_1_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT_1=311,22
PRIMER_RIGHT_1=487,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=60.427
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.6300
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.426682
PRIMER_LEFT_2_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_RIGHT_2_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT_2=308,22
PRIMER_RIGHT_2=487,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=60.427
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.6422
PRIMER_LEFT_3_PENALTY=0.215550
PRIMER_RIGHT_3_PENALTY=0.426682
PRIMER_LEFT_3_SEQUENCE=ACCTCCACCAGTTCACACCTAT
PRIMER_RIGHT_3_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT_3=198,22
PRIMER_RIGHT_3=487,22
PRIMER_LEFT_3_TM=59.784
PRIMER_RIGHT_3_TM=60.427
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.6440
PRIMER_LEFT_4_PENALTY=0.169745
PRIMER_RIGHT_4_PENALTY=0.474295
PRIMER_LEFT_4_SEQUENCE=CACCCAGTCTACCACTCTCCTC
PRIMER_RIGHT_4_SEQUENCE=TGTATGGCTTCTTGTGTGGTGT
PRIMER_LEFT_4=280,22
PRIMER_RIGHT_4=577,22
PRIMER_LEFT_4_TM=60.170
PRIMER_RIGHT_4_TM=60.474
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_02782 Ps_contig_mira-and-tgicl-ass_franssen_24766
SEQUENCE=CGGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGTTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCTCCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTATCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCAGTTGTTTGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTAATAAAGT
TARGET=398,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5334
PRIMER_LEFT_PENALTY=0.246726
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT=373,22
PRIMER_RIGHT=591,22
PRIMER_LEFT_TM=60.247
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.6300
PRIMER_LEFT_1_PENALTY=0.203343
PRIMER_RIGHT_1_PENALTY=0.426682
PRIMER_LEFT_1_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_RIGHT_1_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT_1=311,22
PRIMER_RIGHT_1=487,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=60.427
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.6300
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.426682
PRIMER_LEFT_2_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_RIGHT_2_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT_2=308,22
PRIMER_RIGHT_2=487,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=60.427
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.6422
PRIMER_LEFT_3_PENALTY=0.215550
PRIMER_RIGHT_3_PENALTY=0.426682
PRIMER_LEFT_3_SEQUENCE=ACCTCCACCAGTTCACACCTAT
PRIMER_RIGHT_3_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT_3=198,22
PRIMER_RIGHT_3=487,22
PRIMER_LEFT_3_TM=59.784
PRIMER_RIGHT_3_TM=60.427
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.6440
PRIMER_LEFT_4_PENALTY=0.169745
PRIMER_RIGHT_4_PENALTY=0.474295
PRIMER_LEFT_4_SEQUENCE=CACCCAGTCTACCACTCTCCTC
PRIMER_RIGHT_4_SEQUENCE=TGTATGGCTTCTTGTGTGGTGT
PRIMER_LEFT_4=280,22
PRIMER_RIGHT_4=577,22
PRIMER_LEFT_4_TM=60.170
PRIMER_RIGHT_4_TM=60.474
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_02783 Ps_contig_mira-and-tgicl-ass_franssen_24766
SEQUENCE=CGGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGTTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCTCCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTATCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCAGTTGTTTGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTAATAAAGT
TARGET=440,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5334
PRIMER_LEFT_PENALTY=0.246726
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT=373,22
PRIMER_RIGHT=591,22
PRIMER_LEFT_TM=60.247
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.6300
PRIMER_LEFT_1_PENALTY=0.203343
PRIMER_RIGHT_1_PENALTY=0.426682
PRIMER_LEFT_1_SEQUENCE=CACCACACAAGAAGCCTTACAA
PRIMER_RIGHT_1_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT_1=311,22
PRIMER_RIGHT_1=487,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=60.427
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.6300
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.426682
PRIMER_LEFT_2_SEQUENCE=CAACACCACACAAGAAGCCTTA
PRIMER_RIGHT_2_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT_2=308,22
PRIMER_RIGHT_2=487,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=60.427
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.6422
PRIMER_LEFT_3_PENALTY=0.215550
PRIMER_RIGHT_3_PENALTY=0.426682
PRIMER_LEFT_3_SEQUENCE=ACCTCCACCAGTTCACACCTAT
PRIMER_RIGHT_3_SEQUENCE=AGTGGTAGACAGGGTGAGGATG
PRIMER_LEFT_3=198,22
PRIMER_RIGHT_3=487,22
PRIMER_LEFT_3_TM=59.784
PRIMER_RIGHT_3_TM=60.427
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.6440
PRIMER_LEFT_4_PENALTY=0.169745
PRIMER_RIGHT_4_PENALTY=0.474295
PRIMER_LEFT_4_SEQUENCE=CACCCAGTCTACCACTCTCCTC
PRIMER_RIGHT_4_SEQUENCE=TGTATGGCTTCTTGTGTGGTGT
PRIMER_LEFT_4=280,22
PRIMER_RIGHT_4=577,22
PRIMER_LEFT_4_TM=60.170
PRIMER_RIGHT_4_TM=60.474
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_02784 Ps_contig_mira-and-tgicl-ass_franssen_24766
SEQUENCE=CGGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGTTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCTCCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTATCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCAGTTGTTTGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTAATAAAGT
TARGET=491,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5334
PRIMER_LEFT_PENALTY=0.246726
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT=373,22
PRIMER_RIGHT=591,22
PRIMER_LEFT_TM=60.247
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.5627
PRIMER_LEFT_1_PENALTY=0.426682
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_1_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT_1=466,22
PRIMER_RIGHT_1=713,22
PRIMER_LEFT_1_TM=60.427
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.5627
PRIMER_LEFT_2_PENALTY=0.426682
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_2_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_2=466,22
PRIMER_RIGHT_2=714,22
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.5627
PRIMER_LEFT_3_PENALTY=0.426682
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_3_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_LEFT_3=466,22
PRIMER_RIGHT_3=712,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.6440
PRIMER_LEFT_4_PENALTY=0.169745
PRIMER_RIGHT_4_PENALTY=0.474295
PRIMER_LEFT_4_SEQUENCE=CACCCAGTCTACCACTCTCCTC
PRIMER_RIGHT_4_SEQUENCE=TGTATGGCTTCTTGTGTGGTGT
PRIMER_LEFT_4=280,22
PRIMER_RIGHT_4=577,22
PRIMER_LEFT_4_TM=60.170
PRIMER_RIGHT_4_TM=60.474
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_02785 Ps_contig_mira-and-tgicl-ass_franssen_24766
SEQUENCE=CGGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGTTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCTCCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTATCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCAGTTGTTTGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTAATAAAGT
TARGET=542,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5334
PRIMER_LEFT_PENALTY=0.246726
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=CATCCTCACCCTGTCTACCATT
PRIMER_RIGHT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_LEFT=373,22
PRIMER_RIGHT=591,22
PRIMER_LEFT_TM=60.247
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.5627
PRIMER_LEFT_1_PENALTY=0.426682
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_1_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT_1=466,22
PRIMER_RIGHT_1=713,22
PRIMER_LEFT_1_TM=60.427
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.5627
PRIMER_LEFT_2_PENALTY=0.426682
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_2_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_2=466,22
PRIMER_RIGHT_2=714,22
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.5627
PRIMER_LEFT_3_PENALTY=0.426682
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_3_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_LEFT_3=466,22
PRIMER_RIGHT_3=712,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.6498
PRIMER_LEFT_4_PENALTY=0.513776
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=ATCCTCACCCTGTCTACCACTC
PRIMER_RIGHT_4_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_4=467,22
PRIMER_RIGHT_4=714,22
PRIMER_LEFT_4_TM=59.486
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_02786 Ps_contig_mira-and-tgicl-ass_franssen_24766
SEQUENCE=CGGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGTTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCTCCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTATCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCAGTTGTTTGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTAATAAAGT
TARGET=588,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5155
PRIMER_LEFT_PENALTY=0.474295
PRIMER_RIGHT_PENALTY=0.041227
PRIMER_LEFT_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_SEQUENCE=GAAGAATCCCCTCGATCTTCTT
PRIMER_LEFT=556,22
PRIMER_RIGHT=815,22
PRIMER_LEFT_TM=60.474
PRIMER_RIGHT_TM=60.041
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.5407
PRIMER_LEFT_1_PENALTY=0.474295
PRIMER_RIGHT_1_PENALTY=0.066394
PRIMER_LEFT_1_SEQUENCE=ACACCACACAAGAAGCCATACA
PRIMER_RIGHT_1_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT_1=556,22
PRIMER_RIGHT_1=786,22
PRIMER_LEFT_1_TM=60.474
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.5627
PRIMER_LEFT_2_PENALTY=0.426682
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_2_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_LEFT_2=466,22
PRIMER_RIGHT_2=713,22
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=0.5627
PRIMER_LEFT_3_PENALTY=0.426682
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_3_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_LEFT_3=466,22
PRIMER_RIGHT_3=714,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.5627
PRIMER_LEFT_4_PENALTY=0.426682
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=CATCCTCACCCTGTCTACCACT
PRIMER_RIGHT_4_SEQUENCE=AAGGTGGAGGAGGTGATTTGTA
PRIMER_LEFT_4=466,22
PRIMER_RIGHT_4=712,22
PRIMER_LEFT_4_TM=60.427
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_02787 Ps_contig_mira-and-tgicl-ass_franssen_24766
SEQUENCE=CGGAGATAACCAAAACACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTCTTTTTCAGTTTCCCATCTGAAATTTCAGCAAACCAATATTCTTATTCCTCACCACCACCACCGGTCCACTCACCACCGCCACCAAAGGATCCATACCATTACTCATCTCCTCCACCTCCACCAGTTCACACCTATCCTCACCCACACCCAGTCTACCACTCTCCACCACCACCGGTTCACACCTACCCTCACCCACACCCAGTCTACCACTCTCCTCCTCCACCAACACCACACAAGAAGCCTTACAAGTACCCATCTCCTCCACCACCACCAGTGCACACCTATCCCCATCCTCACCCTGTCTACCATTCCCCACCACCACCACATAAGAAACCATACAAGTACTCATCTCCCCCACCACCACCAGTGCACACCTACCCTCATCCTCACCCTGTCTACCACTCCCCACCACCACCAGTGCACACCTACCCTCACCCTCACCCTGTCTACCACTCCCCACCACCACCACCAACACCACACAAGAAGCCATACAAGTATCCATCACCACCACCACCACCAGCTCACACCTACCCTCCTCATGTTCCTACCCCAGTTTACCATTCTCCTCCTCCTCCGGTTTACTCCCCACCTCCACCAGCCTATTACTACAAATCACCTCCTCCACCTTACCACCACTAGATAGCCAATCATTGGCATCACACTATATGGATCTAAGCAGAAAGTGTGAAAGCAAAGAAAGCCATGCACAAAGAAGATCGAGGGGATTCTTCACATATATATTTCGTCAATGTTTGATTAATATTGTTTCGGGGGCTGCTTTTATTTTTTTAATTTGTGTTTTTGATGTATTAGCCACAGCTGTAAGGCACTGCCCTCAATATGCATGTGTCTGCAGTTGTTTGCATGAATTGTAATTTTCAAAGTTCGGTTTTGTATCAAGCAAGCCAAATAAATATATCCTCTATTAATAAAGT
TARGET=648,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3279
PRIMER_LEFT_PENALTY=0.286660
PRIMER_RIGHT_PENALTY=0.041227
PRIMER_LEFT_SEQUENCE=GCCATACAAGTATCCATCACCA
PRIMER_RIGHT_SEQUENCE=GAAGAATCCCCTCGATCTTCTT
PRIMER_LEFT=570,22
PRIMER_RIGHT=815,22
PRIMER_LEFT_TM=59.713
PRIMER_RIGHT_TM=60.041
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.3531
PRIMER_LEFT_1_PENALTY=0.286660
PRIMER_RIGHT_1_PENALTY=0.066394
PRIMER_LEFT_1_SEQUENCE=GCCATACAAGTATCCATCACCA
PRIMER_RIGHT_1_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_LEFT_1=570,22
PRIMER_RIGHT_1=786,22
PRIMER_LEFT_1_TM=59.713
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.4099
PRIMER_LEFT_2_PENALTY=0.368624
PRIMER_RIGHT_2_PENALTY=0.041227
PRIMER_LEFT_2_SEQUENCE=ATGTTCCTACCCCAGTTTACCA
PRIMER_RIGHT_2_SEQUENCE=GAAGAATCCCCTCGATCTTCTT
PRIMER_LEFT_2=623,22
PRIMER_RIGHT_2=815,22
PRIMER_LEFT_2_TM=59.631
PRIMER_RIGHT_2_TM=60.041
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=193
PRIMER_PAIR_PENALTY_3=0.4099
PRIMER_LEFT_3_PENALTY=0.368624
PRIMER_RIGHT_3_PENALTY=0.041227
PRIMER_LEFT_3_SEQUENCE=TGTTCCTACCCCAGTTTACCAT
PRIMER_RIGHT_3_SEQUENCE=GAAGAATCCCCTCGATCTTCTT
PRIMER_LEFT_3=624,22
PRIMER_RIGHT_3=815,22
PRIMER_LEFT_3_TM=59.631
PRIMER_RIGHT_3_TM=60.041
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=0.4099
PRIMER_LEFT_4_PENALTY=0.368624
PRIMER_RIGHT_4_PENALTY=0.041227
PRIMER_LEFT_4_SEQUENCE=CATGTTCCTACCCCAGTTTACC
PRIMER_RIGHT_4_SEQUENCE=GAAGAATCCCCTCGATCTTCTT
PRIMER_LEFT_4=622,22
PRIMER_RIGHT_4=815,22
PRIMER_LEFT_4_TM=59.631
PRIMER_RIGHT_4_TM=60.041
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_02788 Ps_contig_mira-and-tgicl-ass_franssen_24774
SEQUENCE=ATCAACGCAGTAGTCGGGGATCTTTAAAAATTATCACTCTTAACTAGTCACATTACATCCTTTTCTTTTATGATGATGACCGTCATGATTTCAATAATACCAATCATTTTGATTCACACTTTATTTTATGTTGGATTTTCTGATGACATGCCACTTACCACTACACTTCCACAATCACCAACATCACCTCTCCCACATTTTCACTTGTACAATCAAAATTCCAATTCATATCCTTCATCTACACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCTAGGAGAAGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAAGCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGAAGGCATAAATGTGAACGATTTTGTTCCTATAAAGACAAGACTCAATGAAAATTGGCCGGCGTTATTTAAAAAGGATCATCAAGAAGATGCTAACATTCAATTATGGGTTAATCAATGGTATGCTCACGGAACTTGTTCAGATCAATTGTTCAAGTTTATTTCTTACTTTGAAGAAACATTAAACGTTTATGATAGACATATTATATTAGATATCCTTGAAAAGAATGGTACAAAACCAGGTGGAACATATCCTAAACAAAATATTCTCAATGCAATTCAGACACATACTCTATTTAGGCCACAAATTCGATGTGAGCGGATTGATAATTTGGATTATTTATATGAAATAAGACTTTGTTTGACACCTACTCTAAAACTAGAGTATAAAGATTGTGAAATTCCTTATAGTGGTTGTCGCGATACAGAAGTGTATTTCTAATAAGTTATAATGTTATTGATGGACATTTGATCTTGGTGCTTTGGAAGTTTGGAGGCAGCCAAAGGTTCGTGGGAGTATGATGTTTATCCTTAATTTTAGTTTCCTATTATTCAGTTTTTTCGCTTTTCTTTTGTACTTTTTTGTTATGTTGGGTGTTGTTTTAGTTCTAAATACCTTGTTTACTATAATTGTGTCTCTATTGTCTTGTTTGTACTCTTTCTATTTTCCTCTTTCATATGTATTGTTTGTACTCTTTCTATTTTCCTCTTTCATATGTATTGTTTGTACTCTTTCTATTTCCCTCTTTCACATTTTGTGTTAGAAGGAATTTATATGAATAAAATGTGATGTTTAGTTAGAAAATAG
TARGET=292,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1440
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=TTCTGATGACATGCCACTTACC
PRIMER_RIGHT_SEQUENCE=AATAAAAGAGGTGGTGGTGGTG
PRIMER_LEFT=137,22
PRIMER_RIGHT=418,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.2489
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.248820
PRIMER_LEFT_1_SEQUENCE=TTCTGATGACATGCCACTTACC
PRIMER_RIGHT_1_SEQUENCE=TCTTATCCTAGGTGGTGGTGGT
PRIMER_LEFT_1=137,22
PRIMER_RIGHT_1=368,22
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=59.751
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.4195
PRIMER_LEFT_2_PENALTY=0.233224
PRIMER_RIGHT_2_PENALTY=0.186309
PRIMER_LEFT_2_SEQUENCE=ATCACCTCTCCCACATTTTCAC
PRIMER_RIGHT_2_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_2=182,22
PRIMER_RIGHT_2=468,22
PRIMER_LEFT_2_TM=60.233
PRIMER_RIGHT_2_TM=60.186
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.4195
PRIMER_LEFT_3_PENALTY=0.233224
PRIMER_RIGHT_3_PENALTY=0.186309
PRIMER_LEFT_3_SEQUENCE=ACATCACCTCTCCCACATTTTC
PRIMER_RIGHT_3_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_3=180,22
PRIMER_RIGHT_3=468,22
PRIMER_LEFT_3_TM=60.233
PRIMER_RIGHT_3_TM=60.186
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=0.4514
PRIMER_LEFT_4_PENALTY=0.265053
PRIMER_RIGHT_4_PENALTY=0.186309
PRIMER_LEFT_4_SEQUENCE=AACATCACCTCTCCCACATTTT
PRIMER_RIGHT_4_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_4=179,22
PRIMER_RIGHT_4=468,22
PRIMER_LEFT_4_TM=59.735
PRIMER_RIGHT_4_TM=60.186
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_02789 Ps_contig_mira-and-tgicl-ass_franssen_24774
SEQUENCE=ATCAACGCAGTAGTCGGGGATCTTTAAAAATTATCACTCTTAACTAGTCACATTACATCCTTTTCTTTTATGATGATGACCGTCATGATTTCAATAATACCAATCATTTTGATTCACACTTTATTTTATGTTGGATTTTCTGATGACATGCCACTTACCACTACACTTCCACAATCACCAACATCACCTCTCCCACATTTTCACTTGTACAATCAAAATTCCAATTCATATCCTTCATCTACACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCTAGGAGAAGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAAGCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGAAGGCATAAATGTGAACGATTTTGTTCCTATAAAGACAAGACTCAATGAAAATTGGCCGGCGTTATTTAAAAAGGATCATCAAGAAGATGCTAACATTCAATTATGGGTTAATCAATGGTATGCTCACGGAACTTGTTCAGATCAATTGTTCAAGTTTATTTCTTACTTTGAAGAAACATTAAACGTTTATGATAGACATATTATATTAGATATCCTTGAAAAGAATGGTACAAAACCAGGTGGAACATATCCTAAACAAAATATTCTCAATGCAATTCAGACACATACTCTATTTAGGCCACAAATTCGATGTGAGCGGATTGATAATTTGGATTATTTATATGAAATAAGACTTTGTTTGACACCTACTCTAAAACTAGAGTATAAAGATTGTGAAATTCCTTATAGTGGTTGTCGCGATACAGAAGTGTATTTCTAATAAGTTATAATGTTATTGATGGACATTTGATCTTGGTGCTTTGGAAGTTTGGAGGCAGCCAAAGGTTCGTGGGAGTATGATGTTTATCCTTAATTTTAGTTTCCTATTATTCAGTTTTTTCGCTTTTCTTTTGTACTTTTTTGTTATGTTGGGTGTTGTTTTAGTTCTAAATACCTTGTTTACTATAATTGTGTCTCTATTGTCTTGTTTGTACTCTTTCTATTTTCCTCTTTCATATGTATTGTTTGTACTCTTTCTATTTTCCTCTTTCATATGTATTGTTTGTACTCTTTCTATTTCCCTCTTTCACATTTTGTGTTAGAAGGAATTTATATGAATAAAATGTGATGTTTAGTTAGAAAATAG
TARGET=346,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1440
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=TTCTGATGACATGCCACTTACC
PRIMER_RIGHT_SEQUENCE=AATAAAAGAGGTGGTGGTGGTG
PRIMER_LEFT=137,22
PRIMER_RIGHT=418,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.4195
PRIMER_LEFT_1_PENALTY=0.233224
PRIMER_RIGHT_1_PENALTY=0.186309
PRIMER_LEFT_1_SEQUENCE=ATCACCTCTCCCACATTTTCAC
PRIMER_RIGHT_1_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_1=182,22
PRIMER_RIGHT_1=468,22
PRIMER_LEFT_1_TM=60.233
PRIMER_RIGHT_1_TM=60.186
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.4195
PRIMER_LEFT_2_PENALTY=0.233224
PRIMER_RIGHT_2_PENALTY=0.186309
PRIMER_LEFT_2_SEQUENCE=ACATCACCTCTCCCACATTTTC
PRIMER_RIGHT_2_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_2=180,22
PRIMER_RIGHT_2=468,22
PRIMER_LEFT_2_TM=60.233
PRIMER_RIGHT_2_TM=60.186
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.4514
PRIMER_LEFT_3_PENALTY=0.265053
PRIMER_RIGHT_3_PENALTY=0.186309
PRIMER_LEFT_3_SEQUENCE=AACATCACCTCTCCCACATTTT
PRIMER_RIGHT_3_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_3=179,22
PRIMER_RIGHT_3=468,22
PRIMER_LEFT_3_TM=59.735
PRIMER_RIGHT_3_TM=60.186
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.5386
PRIMER_LEFT_4_PENALTY=0.394672
PRIMER_RIGHT_4_PENALTY=0.143968
PRIMER_LEFT_4_SEQUENCE=CCTCTCCCACATTTTCACTTGT
PRIMER_RIGHT_4_SEQUENCE=AATAAAAGAGGTGGTGGTGGTG
PRIMER_LEFT_4=186,22
PRIMER_RIGHT_4=418,22
PRIMER_LEFT_4_TM=60.395
PRIMER_RIGHT_4_TM=60.144
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_02790 Ps_contig_mira-and-tgicl-ass_franssen_24774
SEQUENCE=ATCAACGCAGTAGTCGGGGATCTTTAAAAATTATCACTCTTAACTAGTCACATTACATCCTTTTCTTTTATGATGATGACCGTCATGATTTCAATAATACCAATCATTTTGATTCACACTTTATTTTATGTTGGATTTTCTGATGACATGCCACTTACCACTACACTTCCACAATCACCAACATCACCTCTCCCACATTTTCACTTGTACAATCAAAATTCCAATTCATATCCTTCATCTACACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCTAGGAGAAGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAAGCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGAAGGCATAAATGTGAACGATTTTGTTCCTATAAAGACAAGACTCAATGAAAATTGGCCGGCGTTATTTAAAAAGGATCATCAAGAAGATGCTAACATTCAATTATGGGTTAATCAATGGTATGCTCACGGAACTTGTTCAGATCAATTGTTCAAGTTTATTTCTTACTTTGAAGAAACATTAAACGTTTATGATAGACATATTATATTAGATATCCTTGAAAAGAATGGTACAAAACCAGGTGGAACATATCCTAAACAAAATATTCTCAATGCAATTCAGACACATACTCTATTTAGGCCACAAATTCGATGTGAGCGGATTGATAATTTGGATTATTTATATGAAATAAGACTTTGTTTGACACCTACTCTAAAACTAGAGTATAAAGATTGTGAAATTCCTTATAGTGGTTGTCGCGATACAGAAGTGTATTTCTAATAAGTTATAATGTTATTGATGGACATTTGATCTTGGTGCTTTGGAAGTTTGGAGGCAGCCAAAGGTTCGTGGGAGTATGATGTTTATCCTTAATTTTAGTTTCCTATTATTCAGTTTTTTCGCTTTTCTTTTGTACTTTTTTGTTATGTTGGGTGTTGTTTTAGTTCTAAATACCTTGTTTACTATAATTGTGTCTCTATTGTCTTGTTTGTACTCTTTCTATTTTCCTCTTTCATATGTATTGTTTGTACTCTTTCTATTTTCCTCTTTCATATGTATTGTTTGTACTCTTTCTATTTCCCTCTTTCACATTTTGTGTTAGAAGGAATTTATATGAATAAAATGTGATGTTTAGTTAGAAAATAG
TARGET=388,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3132
PRIMER_LEFT_PENALTY=0.248820
PRIMER_RIGHT_PENALTY=0.064372
PRIMER_LEFT_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_RIGHT_SEQUENCE=GGCCATAACCCATGGATAGTAA
PRIMER_LEFT=347,22
PRIMER_RIGHT=526,22
PRIMER_LEFT_TM=59.751
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=0.3886
PRIMER_LEFT_1_PENALTY=0.248820
PRIMER_RIGHT_1_PENALTY=0.139753
PRIMER_LEFT_1_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_RIGHT_1_SEQUENCE=TGCAATCTCGTATCTCAACACC
PRIMER_LEFT_1=347,22
PRIMER_RIGHT_1=558,22
PRIMER_LEFT_1_TM=59.751
PRIMER_RIGHT_1_TM=60.140
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.4195
PRIMER_LEFT_2_PENALTY=0.233224
PRIMER_RIGHT_2_PENALTY=0.186309
PRIMER_LEFT_2_SEQUENCE=ATCACCTCTCCCACATTTTCAC
PRIMER_RIGHT_2_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_2=182,22
PRIMER_RIGHT_2=468,22
PRIMER_LEFT_2_TM=60.233
PRIMER_RIGHT_2_TM=60.186
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.4195
PRIMER_LEFT_3_PENALTY=0.233224
PRIMER_RIGHT_3_PENALTY=0.186309
PRIMER_LEFT_3_SEQUENCE=ACATCACCTCTCCCACATTTTC
PRIMER_RIGHT_3_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_3=180,22
PRIMER_RIGHT_3=468,22
PRIMER_LEFT_3_TM=60.233
PRIMER_RIGHT_3_TM=60.186
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=0.4514
PRIMER_LEFT_4_PENALTY=0.265053
PRIMER_RIGHT_4_PENALTY=0.186309
PRIMER_LEFT_4_SEQUENCE=AACATCACCTCTCCCACATTTT
PRIMER_RIGHT_4_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_4=179,22
PRIMER_RIGHT_4=468,22
PRIMER_LEFT_4_TM=59.735
PRIMER_RIGHT_4_TM=60.186
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_02791 Ps_contig_mira-and-tgicl-ass_franssen_24776
SEQUENCE=CGATCTTCTTTGTGCATGGCTTTCTTTTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATTGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTAAACCGGAGGAGGAGGAGAATGGTAAACTGGGGTAGGAACATATGTTTGGTGCTACAAATCAAATTGGTAGGGATAGCCGCGGCGGAACAAGGGCGAGCACTACTTATAGTGCCACCACCAAA
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4350
PRIMER_LEFT_PENALTY=0.066394
PRIMER_RIGHT_PENALTY=0.368624
PRIMER_LEFT_SEQUENCE=GGCTTTCTTTTGCTTTCACACT
PRIMER_RIGHT_SEQUENCE=ATGTTCCTACCCCAGTTTACCA
PRIMER_LEFT=17,22
PRIMER_RIGHT=181,22
PRIMER_LEFT_TM=59.934
PRIMER_RIGHT_TM=59.631
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=0.4350
PRIMER_LEFT_1_PENALTY=0.066394
PRIMER_RIGHT_1_PENALTY=0.368624
PRIMER_LEFT_1_SEQUENCE=GGCTTTCTTTTGCTTTCACACT
PRIMER_RIGHT_1_SEQUENCE=TGTTCCTACCCCAGTTTACCAT
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=180,22
PRIMER_LEFT_1_TM=59.934
PRIMER_RIGHT_1_TM=59.631
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.8545
PRIMER_LEFT_2_PENALTY=0.066394
PRIMER_RIGHT_2_PENALTY=0.788058
PRIMER_LEFT_2_SEQUENCE=GGCTTTCTTTTGCTTTCACACT
PRIMER_RIGHT_2_SEQUENCE=TAAGTAGTGCTCGCCCTTGTTC
PRIMER_LEFT_2=17,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=59.934
PRIMER_RIGHT_2_TM=60.788
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.9241
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.788058
PRIMER_LEFT_3_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_RIGHT_3_SEQUENCE=TAAGTAGTGCTCGCCCTTGTTC
PRIMER_LEFT_3=91,22
PRIMER_RIGHT_3=246,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=60.788
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.9241
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.788058
PRIMER_LEFT_4_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_RIGHT_4_SEQUENCE=TAAGTAGTGCTCGCCCTTGTTC
PRIMER_LEFT_4=90,22
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=60.788
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_02792 Ps_contig_mira-and-tgicl-ass_franssen_24790
SEQUENCE=GTGCRRSGWBGGGACACCATTCCCCTCTCTCTCTCCATACATTATCAATATCTCTTCTCTTTCATTTTCCTCTCTTCAGAATGGGAAGTAATGGAAAAACTAATTATGGAGAATATACCTATGAGAATCTTGAGAGAGAGCCTTATTGGCCATCAGAGAAGCTAGTAAAATYTCCATCACTGGTGCTGGTCGGTTTTATCGCGTCGCACTTAGCGCGGCTCTCAAGGAGGGA
TARGET=25,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02793 Ps_contig_mira-and-tgicl-ass_franssen_24804
SEQUENCE=TCGACGTGTTTGCGGTGGCAATGTCTACCGTTTCCGTATGATCCATTTCGAAAGAACGATAAGTTCAGATGGAGAGAAACAACAACATTGGCCTCTCATCTCTCTGCATTCCTTCTTCTGCAGAGAAGAAGAAGAAAAACTATGATGAACAAAATAAGAATAACAATTATTTATTTGCTGAGAAACTTGAATTGAAGAGTGTGTTAGGTTTTTGACAATC
TARGET=124,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.7884
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=3.780144
PRIMER_LEFT_SEQUENCE=TGGAGAGAAACAACAACATTGG
PRIMER_RIGHT_SEQUENCE=CACTCTTCAATTCAAGTTTCTCAGC
PRIMER_LEFT=69,22
PRIMER_RIGHT=200,25
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.780
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=3.8009
PRIMER_LEFT_1_PENALTY=0.020747
PRIMER_RIGHT_1_PENALTY=3.780144
PRIMER_LEFT_1_SEQUENCE=CCTCTCATCTCTCTGCATTCCT
PRIMER_RIGHT_1_SEQUENCE=CACTCTTCAATTCAAGTTTCTCAGC
PRIMER_LEFT_1=91,22
PRIMER_RIGHT_1=200,25
PRIMER_LEFT_1_TM=59.979
PRIMER_RIGHT_1_TM=60.780
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=110
PRIMER_PAIR_PENALTY_2=3.8200
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=3.780144
PRIMER_LEFT_2_SEQUENCE=AGAAACAACAACATTGGCCTCT
PRIMER_RIGHT_2_SEQUENCE=CACTCTTCAATTCAAGTTTCTCAGC
PRIMER_LEFT_2=74,22
PRIMER_RIGHT_2=200,25
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=60.780
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=127
PRIMER_PAIR_PENALTY_3=3.8200
PRIMER_LEFT_3_PENALTY=0.039879
PRIMER_RIGHT_3_PENALTY=3.780144
PRIMER_LEFT_3_SEQUENCE=AGAGAAACAACAACATTGGCCT
PRIMER_RIGHT_3_SEQUENCE=CACTCTTCAATTCAAGTTTCTCAGC
PRIMER_LEFT_3=72,22
PRIMER_RIGHT_3=200,25
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=60.780
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=3.9192
PRIMER_LEFT_4_PENALTY=0.139083
PRIMER_RIGHT_4_PENALTY=3.780144
PRIMER_LEFT_4_SEQUENCE=ACAACATTGGCCTCTCATCTCT
PRIMER_RIGHT_4_SEQUENCE=CACTCTTCAATTCAAGTTTCTCAGC
PRIMER_LEFT_4=81,22
PRIMER_RIGHT_4=200,25
PRIMER_LEFT_4_TM=60.139
PRIMER_RIGHT_4_TM=60.780
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_02794 Ps_contig_mira-and-tgicl-ass_franssen_24828
SEQUENCE=GATACACCAACATACATACATATCTTCTTCTCAAACTCCTTTTTATCAAATCAATAATCATGGCGTCAATACTAGACTCTTTGATCTTATTTTCCTTGATCACTTTATCATTGGCATTGGACATGTCAAGTGAACGTAGTAACAAGGAGGTTATGACCATGTACGAGAAATGGTTAGTAGAACACCAAAAAGTGTATAACGGATTAGGAGAGAAAGACCAAAGGTTTCAAATATTTAAGGATAATTTAAAATTCATAGATGAGCACAATGCTCAAAATCACAGTTATAGAGTTGGATTGAACGAGTTTGCAGATATAACTAATAAAGAATATCGTGACATGTATTTGAGCAGGAGGATTAATAATAATATGAAAAATAAGATTACCAGTGAACGATATGCTTATAAGGCAGGTGATAATAATAGATTGCCCGTGTCTGTTGATTGGAGAGGGGCTCTTGGTACTATAAAAAATCAAGGAAGTTGTGGAGCTTGTTGGGCGTTTTCCACAGTAGCGGCAGTGGAAGCGATTAACAAGATAGTGACAGGGAGGTTTGTGAATTTGTCTG
TARGET=10,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02795 Ps_contig_mira-and-tgicl-ass_franssen_24832
SEQUENCE=CAACGCAGAGTACGCGGGGAACCATTTCCATTCCATTTCTCACTCCCAATGTCCTCTTCTCTCTCCACCCCTTTCATCCACCATTCTTCAACTCTTCGCCATGGAACCAACAGAAGAAACAACCGAAAAACCAACAGAAACAAACTGCAGCAGGAGAAGCTGTAGATGCTCCCGAAACCCAAGATCCACTTGAAGTTGAGAACAAGGAAGTGGTAACAGAGGAAGCAAAGGAAGAGAACTTAGAAGCACCTAAAGAAACTGAGGAATCGGTTGAAGAAGTGAAGGAAGAGGCGGAAGCAGTAGTGGAGGAGCCAATAAGCAACTACTGAAAATGAGTCAGCCACCACCACCAC
TARGET=341,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02796 Ps_contig_mira-and-tgicl-ass_franssen_24911
SEQUENCE=GACTGTTCCACTTTCTTCATTCCTCAAAACCTCAAACATAGTGAACAAAACCATCTTCATAGACCAAAACCCACTTTGAGAAAACATGGGGCCTCAAATGGCCTCTATTACTCTAACTATTGCATTGACAATAGTTTCCCTCACCTTGCCATCTGAAGCAAATAACTACATCTATTCATCTCCACCACCACCTAAACCTTACTACTACTACTCTCCACCACCACCAGTGCACTCCACCCTTCCCTCCTACCTTACCATTATACGCCTCCAACCCAACCAAAACCCAAAAAAAAACC
TARGET=200,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1075
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.095324
PRIMER_LEFT_SEQUENCE=AGACCAAAACCCACTTTGAGAA
PRIMER_RIGHT_SEQUENCE=GGTTGGAGGCGTATAATGGTAA
PRIMER_LEFT=60,22
PRIMER_RIGHT=272,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.2091
PRIMER_LEFT_1_PENALTY=0.113803
PRIMER_RIGHT_1_PENALTY=0.095324
PRIMER_LEFT_1_SEQUENCE=CCAAAACCCACTTTGAGAAAAC
PRIMER_RIGHT_1_SEQUENCE=GGTTGGAGGCGTATAATGGTAA
PRIMER_LEFT_1=63,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.2250
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=0.212871
PRIMER_LEFT_2_SEQUENCE=AGACCAAAACCCACTTTGAGAA
PRIMER_RIGHT_2_SEQUENCE=TTGGAGGCGTATAATGGTAAGG
PRIMER_LEFT_2=60,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.213
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.2545
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=0.242357
PRIMER_LEFT_3_SEQUENCE=AGACCAAAACCCACTTTGAGAA
PRIMER_RIGHT_3_SEQUENCE=TGGAGGCGTATAATGGTAAGGT
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=269,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=59.758
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=0.2760
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=0.263882
PRIMER_LEFT_4_SEQUENCE=AGACCAAAACCCACTTTGAGAA
PRIMER_RIGHT_4_SEQUENCE=AATGGTAAGGTAGGAGGGAAGG
PRIMER_LEFT_4=60,22
PRIMER_RIGHT_4=258,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.736
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_02797 Ps_contig_mira-and-tgicl-ass_franssen_24911
SEQUENCE=GACTGTTCCACTTTCTTCATTCCTCAAAACCTCAAACATAGTGAACAAAACCATCTTCATAGACCAAAACCCACTTTGAGAAAACATGGGGCCTCAAATGGCCTCTATTACTCTAACTATTGCATTGACAATAGTTTCCCTCACCTTGCCATCTGAAGCAAATAACTACATCTATTCATCTCCACCACCACCTAAACCTTACTACTACTACTCTCCACCACCACCAGTGCACTCCACCCTTCCCTCCTACCTTACCATTATACGCCTCCAACCCAACCAAAACCCAAAAAAAAACC
TARGET=215,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1075
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.095324
PRIMER_LEFT_SEQUENCE=AGACCAAAACCCACTTTGAGAA
PRIMER_RIGHT_SEQUENCE=GGTTGGAGGCGTATAATGGTAA
PRIMER_LEFT=60,22
PRIMER_RIGHT=272,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.2091
PRIMER_LEFT_1_PENALTY=0.113803
PRIMER_RIGHT_1_PENALTY=0.095324
PRIMER_LEFT_1_SEQUENCE=CCAAAACCCACTTTGAGAAAAC
PRIMER_RIGHT_1_SEQUENCE=GGTTGGAGGCGTATAATGGTAA
PRIMER_LEFT_1=63,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.2250
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=0.212871
PRIMER_LEFT_2_SEQUENCE=AGACCAAAACCCACTTTGAGAA
PRIMER_RIGHT_2_SEQUENCE=TTGGAGGCGTATAATGGTAAGG
PRIMER_LEFT_2=60,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.213
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.2545
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=0.242357
PRIMER_LEFT_3_SEQUENCE=AGACCAAAACCCACTTTGAGAA
PRIMER_RIGHT_3_SEQUENCE=TGGAGGCGTATAATGGTAAGGT
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=269,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=59.758
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=0.2760
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=0.263882
PRIMER_LEFT_4_SEQUENCE=AGACCAAAACCCACTTTGAGAA
PRIMER_RIGHT_4_SEQUENCE=AATGGTAAGGTAGGAGGGAAGG
PRIMER_LEFT_4=60,22
PRIMER_RIGHT_4=258,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.736
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_02798 Ps_contig_mira-and-tgicl-ass_franssen_24916
SEQUENCE=CTACCAATAGTCAAATTAATTTTTACTTTGATTATTTTCTAGATTCTGTTTTTATTTACTACAATTANAAGTAAGATCAGCATACACTTCCATACAAGAGGGGAAGTTCACATCCACCATGATAAATAAATAAAAAAAGAATGCATTTCATTTGATCCAATAGCTCCGTAACTTACAACTTGTACAAATTATAGCAATATGTTTAAACAAGTGTGGATCTTTGAAATAAAACCCATTAGCATTATTAACCTTCCTCTCCGAACTCCTTGGTGAATCTCTCGTGAACATCGAGGTCAGATTTGCTTACTGTAGGCCTTTGTCTAGCAAGTACTTTGTCAAAATCGGTTCTTGATATAGGTGGAGGAAGGATCTTTGAAGCAAGTCCCTGTGTAGCAAGATCTTGCATTGTAATTTGTACTGCATTTTGTTGCTTTTGTCCACATGGTATCCACATACCTTCTGGACTACTTAAAGAAAACNTGGCATCTTTGGGTTTTAACAACTAGGGTTTACCGAAAACAAAAAACCGTATTTCCCCGTTTACCAACCGGTACC
TARGET=122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2035
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.067516
PRIMER_LEFT_SEQUENCE=ACACTTCCATACAAGAGGGGAA
PRIMER_RIGHT_SEQUENCE=TCAAAGATCCTTCCTCCACCTA
PRIMER_LEFT=83,22
PRIMER_RIGHT=375,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.068
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.2548
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.118777
PRIMER_LEFT_1_SEQUENCE=ACACTTCCATACAAGAGGGGAA
PRIMER_RIGHT_1_SEQUENCE=GATGTTCACGAGAGATTCACCA
PRIMER_LEFT_1=83,22
PRIMER_RIGHT_1=288,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.119
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=0.2751
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.139083
PRIMER_LEFT_2_SEQUENCE=ACACTTCCATACAAGAGGGGAA
PRIMER_RIGHT_2_SEQUENCE=ACCTCGATGTTCACGAGAGATT
PRIMER_LEFT_2=83,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.139
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.2855
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.149516
PRIMER_LEFT_3_SEQUENCE=ACACTTCCATACAAGAGGGGAA
PRIMER_RIGHT_3_SEQUENCE=CCTCCACCTATATCAAGAACCG
PRIMER_LEFT_3=83,22
PRIMER_RIGHT_3=363,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.850
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.3827
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.246726
PRIMER_LEFT_4_SEQUENCE=ACACTTCCATACAAGAGGGGAA
PRIMER_RIGHT_4_SEQUENCE=GAGAGATTCACCAAGGAGTTCG
PRIMER_LEFT_4=83,22
PRIMER_RIGHT_4=279,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=60.247
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=197
=
PRIMER_SEQUENCE_ID=SSR_02799 Ps_contig_mira-and-tgicl-ass_franssen_24922
SEQUENCE=AACTTTAACAACGATGATATTTGATATATTACACATGATAACACATTCACCACTTTTACTCGAAATCAAACTCATCTTCACATATACATAGTGATTTATCTTTACTCTTTTGTTGAAAACCCAACATTATTTATTACACTTAACACATGCATGCATGATGATATAATCAGTTACCATTGTTGAAACTACCACCATGTCCACCTCCACCACGATAACCCACCACCTCCATGGTTCCCGNATCTCTGCGTTGATACCACTTGCTT
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02800 Ps_contig_mira-and-tgicl-ass_franssen_24924
SEQUENCE=ACGCAGAGTCGGGGACCCACTGCCACTAATCTTTCTTTGTTTCAAAACATTCTCTCTCTTGAAAACAATGGAAGCACTGAAGATGAATGTTTTTGTGGCGGTTATGGTTGCTTTGTTGGTGATAGCTACAAGTGGTGTTTCAGCTGCCGATTCACCAGCTCCTAGTCCTGCTTCTGATGCTACTGCACTTTTTGTTCCAAGTGCTGTTGCTTCTCTCATAGCTCTTGCATTTGGGTTTCTCTTTTGATGCTTTAACTATGTGAGTTTGATTTGTGCATAGGATGAAGGATGAAGACACTTTTAGGTTTTGTTTGAAATATGAATTATTATTATTAATGATTTATTATTATTCATATATTCTTATGTAACACATTCATTTATCATTGTACTTTTTTTTATGGGTTGTGACTTGTGACATGTAATATTATTATGATTTGTTTGCTTCTATTCATTTGACTTTCCTCTATTGTTGGTAATGGAGGGTGAAATTTTGATTTTCTTTTTGTTGTTCTTAAATAGTATTCCAACATGAATGTATTTTTTTTTA
TARGET=323,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4522
PRIMER_LEFT_PENALTY=0.040146
PRIMER_RIGHT_PENALTY=0.412035
PRIMER_LEFT_SEQUENCE=TGATTTGTGCATAGGATGAAGG
PRIMER_RIGHT_SEQUENCE=CAAAATTTCACCCTCCATTACC
PRIMER_LEFT=266,22
PRIMER_RIGHT=492,22
PRIMER_LEFT_TM=59.960
PRIMER_RIGHT_TM=59.588
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.5031
PRIMER_LEFT_1_PENALTY=0.040146
PRIMER_RIGHT_1_PENALTY=0.462913
PRIMER_LEFT_1_SEQUENCE=TGATTTGTGCATAGGATGAAGG
PRIMER_RIGHT_1_SEQUENCE=ATTTCACCCTCCATTACCAACA
PRIMER_LEFT_1=266,22
PRIMER_RIGHT_1=488,22
PRIMER_LEFT_1_TM=59.960
PRIMER_RIGHT_1_TM=60.463
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.5031
PRIMER_LEFT_2_PENALTY=0.040146
PRIMER_RIGHT_2_PENALTY=0.462913
PRIMER_LEFT_2_SEQUENCE=TTTGTGCATAGGATGAAGGATG
PRIMER_RIGHT_2_SEQUENCE=ATTTCACCCTCCATTACCAACA
PRIMER_LEFT_2=269,22
PRIMER_RIGHT_2=488,22
PRIMER_LEFT_2_TM=59.960
PRIMER_RIGHT_2_TM=60.463
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.5031
PRIMER_LEFT_3_PENALTY=0.040146
PRIMER_RIGHT_3_PENALTY=0.462913
PRIMER_LEFT_3_SEQUENCE=TGATTTGTGCATAGGATGAAGG
PRIMER_RIGHT_3_SEQUENCE=TTCACCCTCCATTACCAACAAT
PRIMER_LEFT_3=266,22
PRIMER_RIGHT_3=486,22
PRIMER_LEFT_3_TM=59.960
PRIMER_RIGHT_3_TM=60.463
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.5031
PRIMER_LEFT_4_PENALTY=0.040146
PRIMER_RIGHT_4_PENALTY=0.462913
PRIMER_LEFT_4_SEQUENCE=TTTGTGCATAGGATGAAGGATG
PRIMER_RIGHT_4_SEQUENCE=TTCACCCTCCATTACCAACAAT
PRIMER_LEFT_4=269,22
PRIMER_RIGHT_4=486,22
PRIMER_LEFT_4_TM=59.960
PRIMER_RIGHT_4_TM=60.463
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_02801 Ps_contig_mira-and-tgicl-ass_franssen_24925
SEQUENCE=GATAAATAGAGGAGATATAGATTTCGGGGTGATCTTTAAAAATTATCACTCTTAACTAGTCACATTACATCCTTTTCTTTTATGATGATGACCGTCATGATTTCAATAATACCAATCATTTTGATTCACACTTTATTTTATGTTGGATTTTCTGATGACATGCCACTTACCACTACACTTCCACAATCACCAACATCACCTCTCCCACATTTTCACTTGTACAATCAAAATTCCAATTCATATCCTTCATCTACACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCTAGGAGAAGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAAGCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGAAGGCATAAATGTGAACGATTTTGTTCCTATAAAGACAAGACTCAATGAAAATTGGCCGGCGTTATTTAAAAAGGATCATCAAGAAGATGCTAACATTCAATTATGGGTTAATCAATGGTATGCTCACGGAACTTGTTCAGATCAATTGTTCAAGTTTATTTCTTACTTTGAAGAAACATTAAACGTTTATGATAGACATATTATATTAGATATCCTTGAAAAGAATGGTACAAAACCAGGTGGAACATATCCTAAACAAAATATTCTCAATGCAATTCAGACACATACTCTATTTAGGCCACAAATTCGATGTGAGCGGATTGATAATTTGGATTATTTATATGAAATAAGACTTTGTTTGACACCTACTCTAAAACTAGAGTATAAAGATTGTGAAATTCCTTATAGTGGTTGTCGCGATACAGAAGTGTATTTCTAATAAGTTATAATGTTATTGATGGACATTTGATCTTGGTGCTTTGGAAGTTTGGTGGCAGCCAAAGGTTCGTGGGAGCATGATGTTTATCCTTAATTTTAGTTTCCTATTATTCAGTTTTTTCGCTTTTCTTTTGTACTTTTTTGTTATGTTGGGTGTTGTTTTAGTTCTAAATACCTTGTTTACTATAATTGTGTCTCTATTGTCTTGTTTGTACTCTTTCTATTTTCCTCTTTCATATGTATTGTTTGTACTCTTTCTATTTCCCTCTTTCACATTTTGTGTTAGAAGGAATTTATATGAATAAAATGTGATGTTTAAGTTT
TARGET=304,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1440
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=TTCTGATGACATGCCACTTACC
PRIMER_RIGHT_SEQUENCE=AATAAAAGAGGTGGTGGTGGTG
PRIMER_LEFT=149,22
PRIMER_RIGHT=430,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.2489
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.248820
PRIMER_LEFT_1_SEQUENCE=TTCTGATGACATGCCACTTACC
PRIMER_RIGHT_1_SEQUENCE=TCTTATCCTAGGTGGTGGTGGT
PRIMER_LEFT_1=149,22
PRIMER_RIGHT_1=380,22
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=59.751
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.4195
PRIMER_LEFT_2_PENALTY=0.233224
PRIMER_RIGHT_2_PENALTY=0.186309
PRIMER_LEFT_2_SEQUENCE=ATCACCTCTCCCACATTTTCAC
PRIMER_RIGHT_2_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_2=194,22
PRIMER_RIGHT_2=480,22
PRIMER_LEFT_2_TM=60.233
PRIMER_RIGHT_2_TM=60.186
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.4195
PRIMER_LEFT_3_PENALTY=0.233224
PRIMER_RIGHT_3_PENALTY=0.186309
PRIMER_LEFT_3_SEQUENCE=ACATCACCTCTCCCACATTTTC
PRIMER_RIGHT_3_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_3=192,22
PRIMER_RIGHT_3=480,22
PRIMER_LEFT_3_TM=60.233
PRIMER_RIGHT_3_TM=60.186
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=0.4514
PRIMER_LEFT_4_PENALTY=0.265053
PRIMER_RIGHT_4_PENALTY=0.186309
PRIMER_LEFT_4_SEQUENCE=AACATCACCTCTCCCACATTTT
PRIMER_RIGHT_4_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_4=191,22
PRIMER_RIGHT_4=480,22
PRIMER_LEFT_4_TM=59.735
PRIMER_RIGHT_4_TM=60.186
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_02802 Ps_contig_mira-and-tgicl-ass_franssen_24925
SEQUENCE=GATAAATAGAGGAGATATAGATTTCGGGGTGATCTTTAAAAATTATCACTCTTAACTAGTCACATTACATCCTTTTCTTTTATGATGATGACCGTCATGATTTCAATAATACCAATCATTTTGATTCACACTTTATTTTATGTTGGATTTTCTGATGACATGCCACTTACCACTACACTTCCACAATCACCAACATCACCTCTCCCACATTTTCACTTGTACAATCAAAATTCCAATTCATATCCTTCATCTACACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCTAGGAGAAGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAAGCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGAAGGCATAAATGTGAACGATTTTGTTCCTATAAAGACAAGACTCAATGAAAATTGGCCGGCGTTATTTAAAAAGGATCATCAAGAAGATGCTAACATTCAATTATGGGTTAATCAATGGTATGCTCACGGAACTTGTTCAGATCAATTGTTCAAGTTTATTTCTTACTTTGAAGAAACATTAAACGTTTATGATAGACATATTATATTAGATATCCTTGAAAAGAATGGTACAAAACCAGGTGGAACATATCCTAAACAAAATATTCTCAATGCAATTCAGACACATACTCTATTTAGGCCACAAATTCGATGTGAGCGGATTGATAATTTGGATTATTTATATGAAATAAGACTTTGTTTGACACCTACTCTAAAACTAGAGTATAAAGATTGTGAAATTCCTTATAGTGGTTGTCGCGATACAGAAGTGTATTTCTAATAAGTTATAATGTTATTGATGGACATTTGATCTTGGTGCTTTGGAAGTTTGGTGGCAGCCAAAGGTTCGTGGGAGCATGATGTTTATCCTTAATTTTAGTTTCCTATTATTCAGTTTTTTCGCTTTTCTTTTGTACTTTTTTGTTATGTTGGGTGTTGTTTTAGTTCTAAATACCTTGTTTACTATAATTGTGTCTCTATTGTCTTGTTTGTACTCTTTCTATTTTCCTCTTTCATATGTATTGTTTGTACTCTTTCTATTTCCCTCTTTCACATTTTGTGTTAGAAGGAATTTATATGAATAAAATGTGATGTTTAAGTTT
TARGET=358,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1440
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=TTCTGATGACATGCCACTTACC
PRIMER_RIGHT_SEQUENCE=AATAAAAGAGGTGGTGGTGGTG
PRIMER_LEFT=149,22
PRIMER_RIGHT=430,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.4195
PRIMER_LEFT_1_PENALTY=0.233224
PRIMER_RIGHT_1_PENALTY=0.186309
PRIMER_LEFT_1_SEQUENCE=ATCACCTCTCCCACATTTTCAC
PRIMER_RIGHT_1_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_1=194,22
PRIMER_RIGHT_1=480,22
PRIMER_LEFT_1_TM=60.233
PRIMER_RIGHT_1_TM=60.186
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.4195
PRIMER_LEFT_2_PENALTY=0.233224
PRIMER_RIGHT_2_PENALTY=0.186309
PRIMER_LEFT_2_SEQUENCE=ACATCACCTCTCCCACATTTTC
PRIMER_RIGHT_2_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_2=192,22
PRIMER_RIGHT_2=480,22
PRIMER_LEFT_2_TM=60.233
PRIMER_RIGHT_2_TM=60.186
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.4514
PRIMER_LEFT_3_PENALTY=0.265053
PRIMER_RIGHT_3_PENALTY=0.186309
PRIMER_LEFT_3_SEQUENCE=AACATCACCTCTCCCACATTTT
PRIMER_RIGHT_3_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_3=191,22
PRIMER_RIGHT_3=480,22
PRIMER_LEFT_3_TM=59.735
PRIMER_RIGHT_3_TM=60.186
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.5386
PRIMER_LEFT_4_PENALTY=0.394672
PRIMER_RIGHT_4_PENALTY=0.143968
PRIMER_LEFT_4_SEQUENCE=CCTCTCCCACATTTTCACTTGT
PRIMER_RIGHT_4_SEQUENCE=AATAAAAGAGGTGGTGGTGGTG
PRIMER_LEFT_4=198,22
PRIMER_RIGHT_4=430,22
PRIMER_LEFT_4_TM=60.395
PRIMER_RIGHT_4_TM=60.144
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_02803 Ps_contig_mira-and-tgicl-ass_franssen_24925
SEQUENCE=GATAAATAGAGGAGATATAGATTTCGGGGTGATCTTTAAAAATTATCACTCTTAACTAGTCACATTACATCCTTTTCTTTTATGATGATGACCGTCATGATTTCAATAATACCAATCATTTTGATTCACACTTTATTTTATGTTGGATTTTCTGATGACATGCCACTTACCACTACACTTCCACAATCACCAACATCACCTCTCCCACATTTTCACTTGTACAATCAAAATTCCAATTCATATCCTTCATCTACACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCTAGGAGAAGATCTCCACCACCACCACCGAGAAGATCTCCACCACCACCTCCTAGGAGAAGTTCTCCTCCACCACCACCACCTAGGATAAGATCTCCACCACCTCCTCGTCCACCACCACCACCACCACCACCTCTTTTATTCGACCATTTTAAACTGTCTCAGACATGGCCACCAACATACTGCAAGCTTAAAAATAATGATTGTGTTTCTCCATTGCCACAAAAATTTACTATCCATGGGTTATGGCCAAGTAAAGAAGGTGTTGAGATACGAGATTGCAACAAAGAAGGCATAAATGTGAACGATTTTGTTCCTATAAAGACAAGACTCAATGAAAATTGGCCGGCGTTATTTAAAAAGGATCATCAAGAAGATGCTAACATTCAATTATGGGTTAATCAATGGTATGCTCACGGAACTTGTTCAGATCAATTGTTCAAGTTTATTTCTTACTTTGAAGAAACATTAAACGTTTATGATAGACATATTATATTAGATATCCTTGAAAAGAATGGTACAAAACCAGGTGGAACATATCCTAAACAAAATATTCTCAATGCAATTCAGACACATACTCTATTTAGGCCACAAATTCGATGTGAGCGGATTGATAATTTGGATTATTTATATGAAATAAGACTTTGTTTGACACCTACTCTAAAACTAGAGTATAAAGATTGTGAAATTCCTTATAGTGGTTGTCGCGATACAGAAGTGTATTTCTAATAAGTTATAATGTTATTGATGGACATTTGATCTTGGTGCTTTGGAAGTTTGGTGGCAGCCAAAGGTTCGTGGGAGCATGATGTTTATCCTTAATTTTAGTTTCCTATTATTCAGTTTTTTCGCTTTTCTTTTGTACTTTTTTGTTATGTTGGGTGTTGTTTTAGTTCTAAATACCTTGTTTACTATAATTGTGTCTCTATTGTCTTGTTTGTACTCTTTCTATTTTCCTCTTTCATATGTATTGTTTGTACTCTTTCTATTTCCCTCTTTCACATTTTGTGTTAGAAGGAATTTATATGAATAAAATGTGATGTTTAAGTTT
TARGET=400,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3132
PRIMER_LEFT_PENALTY=0.248820
PRIMER_RIGHT_PENALTY=0.064372
PRIMER_LEFT_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_RIGHT_SEQUENCE=GGCCATAACCCATGGATAGTAA
PRIMER_LEFT=359,22
PRIMER_RIGHT=538,22
PRIMER_LEFT_TM=59.751
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=0.3886
PRIMER_LEFT_1_PENALTY=0.248820
PRIMER_RIGHT_1_PENALTY=0.139753
PRIMER_LEFT_1_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_RIGHT_1_SEQUENCE=TGCAATCTCGTATCTCAACACC
PRIMER_LEFT_1=359,22
PRIMER_RIGHT_1=570,22
PRIMER_LEFT_1_TM=59.751
PRIMER_RIGHT_1_TM=60.140
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.4195
PRIMER_LEFT_2_PENALTY=0.233224
PRIMER_RIGHT_2_PENALTY=0.186309
PRIMER_LEFT_2_SEQUENCE=ATCACCTCTCCCACATTTTCAC
PRIMER_RIGHT_2_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_2=194,22
PRIMER_RIGHT_2=480,22
PRIMER_LEFT_2_TM=60.233
PRIMER_RIGHT_2_TM=60.186
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.4195
PRIMER_LEFT_3_PENALTY=0.233224
PRIMER_RIGHT_3_PENALTY=0.186309
PRIMER_LEFT_3_SEQUENCE=ACATCACCTCTCCCACATTTTC
PRIMER_RIGHT_3_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_3=192,22
PRIMER_RIGHT_3=480,22
PRIMER_LEFT_3_TM=60.233
PRIMER_RIGHT_3_TM=60.186
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=0.4514
PRIMER_LEFT_4_PENALTY=0.265053
PRIMER_RIGHT_4_PENALTY=0.186309
PRIMER_LEFT_4_SEQUENCE=AACATCACCTCTCCCACATTTT
PRIMER_RIGHT_4_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_LEFT_4=191,22
PRIMER_RIGHT_4=480,22
PRIMER_LEFT_4_TM=59.735
PRIMER_RIGHT_4_TM=60.186
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_02804 Ps_contig_mira-and-tgicl-ass_franssen_24932
SEQUENCE=GAATKGGGTCTTAGAGTTTCTCTCTGCGCTCTCTCTCTCTCCCTCTCCCTCTCTTCCTCTCACCTTGCCTCTGTGCATTCTCTATCGTTCTTTGCCTTCCATCACCCTCGGCATTTTCCTTCCCAAGATAGTAGAAGATGGCAGACGCTGAGGATATTCAACCTCTTGTTTGTGACAATGGAACTGGAATGGTTAAGGCTGGATTTGCTGGAGATGATGCACCTCGTGCTGTGTTTCCCAGCATTGTAGGTCGTCCTCGTCACACTGGCGTGATGGTTGGCATGGGCCAAAAAGATGCATATGTTGGTGATGAGGCACAGTCTAAGCGTGGTATTTTGACTCTCAAATACCCCATTGAACATGGAATTGTTAGCAATTGGGATGACATGGAAAAGATTTGGCATCACACCTTCTATAATGAGCTTCGTGTTGCTCCAGAAGAACACCCAGTCCTGTTAACTGAGGCTCCTGTTAAACMTGARGGCTCCTCTTAACCCTAAGGCTAATCGTGAGAAGATGACCCAAATCATGTTTGAGACTTTTAATACCCCTGCCATGTATGTAGCTATTCAGGCCGTTCTTTCACTGTACGCCAGTGGTCGTACCACCGGTATTGTGTTGGATTCTGGTGATGGTGTCAGCCACACTGTCCCTATCTACGAGGGTTATGCCCTTCCACATGCCATCCTTCGTCTTGATCTGGCTGGTCGCGACCTGACTGATTTCTTGATGAAGATTTTGACTGAGCGTGGTTATTCTTTTACCACGTCAGCAGAGCGTGAAATTGTTCGTGKTYWTMGTTGATGTGAAAGAAAAGCTGTCATACATTGCCCTTGACTATGAGCAAGAGCTGGAGACAGCGAGGACCAGCTCATCCGTGGAGAAGAGCTACGAGTTGCCTGACGGACAGGTAATCACCATTGGAGATGAGCGTTTCAGATGTCCAGAGGTGCTGTACCAACCATCCATGATAGGAATGGAAGCAGCAGGAATTCACGAGACCACATACAACTCCATCATGAAGTGCGATGTTGATATCAGGAAAGACTTGTACGGTAACATTGTCCTTTCAGGAGGAACAACCATGTTCCCAGGCATTGCTGATAGAATGAGCAAAGAAATTTCTGCTTTGGCCCCAAGTAGCATGAAGATCAAGGTCGTTGCACCACCTGAGAGAAAATACAGTGTCTGGATTGGTGGTTCTATTTTGGCATCCCTTAGCACCTTCCAGCAGATGTGGATTGCAAAGGCAGAATATGATGAATCTGGTCCATCAATTGTGCACAGAAAGTGCTTCTAAATCACTACTACTACTACTTCTGTTTTGAGAGAAAGAGGTTTTATCAAAGGGAAAGACTAGTGTTATGAATCCTCCGGAAACATTGCAACTTGTAGCCCTTTAAATTCATTATTATTTGCTTTTGTTATCATTTGTTCATGTACTTATTCACAATGTTGGAGTGATTATTTGCTGAAAGCGTTAWGTTTTTTTTWGTT
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02805 Ps_contig_mira-and-tgicl-ass_franssen_24932
SEQUENCE=GAATKGGGTCTTAGAGTTTCTCTCTGCGCTCTCTCTCTCTCCCTCTCCCTCTCTTCCTCTCACCTTGCCTCTGTGCATTCTCTATCGTTCTTTGCCTTCCATCACCCTCGGCATTTTCCTTCCCAAGATAGTAGAAGATGGCAGACGCTGAGGATATTCAACCTCTTGTTTGTGACAATGGAACTGGAATGGTTAAGGCTGGATTTGCTGGAGATGATGCACCTCGTGCTGTGTTTCCCAGCATTGTAGGTCGTCCTCGTCACACTGGCGTGATGGTTGGCATGGGCCAAAAAGATGCATATGTTGGTGATGAGGCACAGTCTAAGCGTGGTATTTTGACTCTCAAATACCCCATTGAACATGGAATTGTTAGCAATTGGGATGACATGGAAAAGATTTGGCATCACACCTTCTATAATGAGCTTCGTGTTGCTCCAGAAGAACACCCAGTCCTGTTAACTGAGGCTCCTGTTAAACMTGARGGCTCCTCTTAACCCTAAGGCTAATCGTGAGAAGATGACCCAAATCATGTTTGAGACTTTTAATACCCCTGCCATGTATGTAGCTATTCAGGCCGTTCTTTCACTGTACGCCAGTGGTCGTACCACCGGTATTGTGTTGGATTCTGGTGATGGTGTCAGCCACACTGTCCCTATCTACGAGGGTTATGCCCTTCCACATGCCATCCTTCGTCTTGATCTGGCTGGTCGCGACCTGACTGATTTCTTGATGAAGATTTTGACTGAGCGTGGTTATTCTTTTACCACGTCAGCAGAGCGTGAAATTGTTCGTGKTYWTMGTTGATGTGAAAGAAAAGCTGTCATACATTGCCCTTGACTATGAGCAAGAGCTGGAGACAGCGAGGACCAGCTCATCCGTGGAGAAGAGCTACGAGTTGCCTGACGGACAGGTAATCACCATTGGAGATGAGCGTTTCAGATGTCCAGAGGTGCTGTACCAACCATCCATGATAGGAATGGAAGCAGCAGGAATTCACGAGACCACATACAACTCCATCATGAAGTGCGATGTTGATATCAGGAAAGACTTGTACGGTAACATTGTCCTTTCAGGAGGAACAACCATGTTCCCAGGCATTGCTGATAGAATGAGCAAAGAAATTTCTGCTTTGGCCCCAAGTAGCATGAAGATCAAGGTCGTTGCACCACCTGAGAGAAAATACAGTGTCTGGATTGGTGGTTCTATTTTGGCATCCCTTAGCACCTTCCAGCAGATGTGGATTGCAAAGGCAGAATATGATGAATCTGGTCCATCAATTGTGCACAGAAAGTGCTTCTAAATCACTACTACTACTACTTCTGTTTTGAGAGAAAGAGGTTTTATCAAAGGGAAAGACTAGTGTTATGAATCCTCCGGAAACATTGCAACTTGTAGCCCTTTAAATTCATTATTATTTGCTTTTGTTATCATTTGTTCATGTACTTATTCACAATGTTGGAGTGATTATTTGCTGAAAGCGTTAWGTTTTTTTTWGTT
TARGET=1304,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02806 Ps_contig_mira-and-tgicl-ass_franssen_24937
SEQUENCE=TTCAACGACCAGAAATAACACGGGGCCGAAGTGGCACGTGCCATGACTGGAAATGTTTCTGCACTCAAAACTGTTAAAGACGTTATAAATATAATAATATAAATAAATAAAATGCATGCAGTTATAGCAACTACCGCTGTATCTATGTTGTATTTGAATAAGTTATGTGTGTACTCATCGTGTATAACCTACTAGTTATGCACTTTTATCTTATGGAATAAAACTTTAATCAATAAATTACCTTGGAACCC
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5265
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=1.494171
PRIMER_LEFT_SEQUENCE=TTCAACGACCAGAAATAACACG
PRIMER_RIGHT_SEQUENCE=GCGGTAGTTGCTATAACTGCAT
PRIMER_LEFT=0,22
PRIMER_RIGHT=136,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=58.506
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=1.6250
PRIMER_LEFT_1_PENALTY=0.032347
PRIMER_RIGHT_1_PENALTY=1.592664
PRIMER_LEFT_1_SEQUENCE=TTCAACGACCAGAAATAACACG
PRIMER_RIGHT_1_SEQUENCE=AGCGGTAGTTGCTATAACTGCAT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=137,23
PRIMER_LEFT_1_TM=60.032
PRIMER_RIGHT_1_TM=59.407
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=2.1341
PRIMER_LEFT_2_PENALTY=0.032347
PRIMER_RIGHT_2_PENALTY=2.101745
PRIMER_LEFT_2_SEQUENCE=TTCAACGACCAGAAATAACACG
PRIMER_RIGHT_2_SEQUENCE=CAACATAGATACAGCGGTAGTTGC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=149,24
PRIMER_LEFT_2_TM=60.032
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.833
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=2.3216
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=2.101745
PRIMER_LEFT_3_SEQUENCE=ACTGGAAATGTTTCTGCACTCA
PRIMER_RIGHT_3_SEQUENCE=CAACATAGATACAGCGGTAGTTGC
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=149,24
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.833
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=2.3216
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=2.101745
PRIMER_LEFT_4_SEQUENCE=TGACTGGAAATGTTTCTGCACT
PRIMER_RIGHT_4_SEQUENCE=CAACATAGATACAGCGGTAGTTGC
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=149,24
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.833
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=107
=
PRIMER_SEQUENCE_ID=SSR_02807 Ps_contig_mira-and-tgicl-ass_franssen_24939
SEQUENCE=AGGTAAAATAGATTTTTGTTGCAGGAATAGGAACTGGTGGAACTATTTCTGGAGCCGGTAGATTCCTAAAACAACAAAATTCAAAAGTACAGGTTATTGGTGTAGAGCCTCTTGAAAGCAACATACTCTCAGGTGGAAAGCCTAGGTACCTTCTACAAAATTCAGGGTATTGGGGCTGGTTTTGTGCCCGGGAATTTGGATGAAGAAGTGGTTGATGAAGTTATAGCGATATCTAGTGAATGTAAGCTATTGAAACTACAAAGCAAATAGCACTACAAAGAAGGCTTGCTGGTGGAATTTCTCAGGTNCTGCTGCTGCTGCAGCTAGTTGACGAGGTGACTAAAGGCGACTAGAAAGTAGGATNTTAGT
TARGET=309,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2399
PRIMER_LEFT_PENALTY=0.056343
PRIMER_RIGHT_PENALTY=0.183585
PRIMER_LEFT_SEQUENCE=TACAAAATTCAGGGTATTGGGG
PRIMER_RIGHT_SEQUENCE=GCCTTTAGTCACCTCGTCAACT
PRIMER_LEFT=153,22
PRIMER_RIGHT=346,22
PRIMER_LEFT_TM=59.944
PRIMER_RIGHT_TM=59.816
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=0.3443
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.315086
PRIMER_LEFT_1_SEQUENCE=TTGGATGAAGAAGTGGTTGATG
PRIMER_RIGHT_1_SEQUENCE=TAGTCGCCTTTAGTCACCTCGT
PRIMER_LEFT_1=195,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=60.315
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=0.3714
PRIMER_LEFT_2_PENALTY=0.056343
PRIMER_RIGHT_2_PENALTY=0.315086
PRIMER_LEFT_2_SEQUENCE=TACAAAATTCAGGGTATTGGGG
PRIMER_RIGHT_2_SEQUENCE=TAGTCGCCTTTAGTCACCTCGT
PRIMER_LEFT_2=153,22
PRIMER_RIGHT_2=351,22
PRIMER_LEFT_2_TM=59.944
PRIMER_RIGHT_2_TM=60.315
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=0.4106
PRIMER_LEFT_3_PENALTY=0.226971
PRIMER_RIGHT_3_PENALTY=0.183585
PRIMER_LEFT_3_SEQUENCE=CATACTCTCAGGTGGAAAGCCT
PRIMER_RIGHT_3_SEQUENCE=GCCTTTAGTCACCTCGTCAACT
PRIMER_LEFT_3=121,22
PRIMER_RIGHT_3=346,22
PRIMER_LEFT_3_TM=59.773
PRIMER_RIGHT_3_TM=59.816
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.4381
PRIMER_LEFT_4_PENALTY=0.254467
PRIMER_RIGHT_4_PENALTY=0.183585
PRIMER_LEFT_4_SEQUENCE=TCTCAGGTGGAAAGCCTAGGTA
PRIMER_RIGHT_4_SEQUENCE=GCCTTTAGTCACCTCGTCAACT
PRIMER_LEFT_4=126,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=60.254
PRIMER_RIGHT_4_TM=59.816
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_02808 Ps_contig_mira-and-tgicl-ass_franssen_24942
SEQUENCE=AAGCAGCGGTATCAACGCAGAGTTTCTTCATTTGTTTTGTTATGAATTATTATTATTCTCATTACTATTAGTGTGCTTTGTTTTTGAATTTTAGACAGCTGTAAGGCACTGTGTGTAGTAAATTATGAATGTATTTTGTTTGGAACCTACTCGTTTTCAAGGGGTAGAAGAAAAATAAAATTAGAAAATTCTAACCTTAAAAAA
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1825
PRIMER_LEFT_PENALTY=0.080323
PRIMER_RIGHT_PENALTY=0.102139
PRIMER_LEFT_SEQUENCE=CAACGCAGAGTTTCTTCATTTG
PRIMER_RIGHT_SEQUENCE=TTCTTCTACCCCTTGAAAACGA
PRIMER_LEFT=12,22
PRIMER_RIGHT=171,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=0.3236
PRIMER_LEFT_1_PENALTY=0.080323
PRIMER_RIGHT_1_PENALTY=0.243298
PRIMER_LEFT_1_SEQUENCE=CAACGCAGAGTTTCTTCATTTG
PRIMER_RIGHT_1_SEQUENCE=TCTTCTACCCCTTGAAAACGAG
PRIMER_LEFT_1=12,22
PRIMER_RIGHT_1=170,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.757
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.3470
PRIMER_LEFT_2_PENALTY=0.080323
PRIMER_RIGHT_2_PENALTY=0.266650
PRIMER_LEFT_2_SEQUENCE=CAACGCAGAGTTTCTTCATTTG
PRIMER_RIGHT_2_SEQUENCE=ACCCCTTGAAAACGAGTAGGTT
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=164,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=60.267
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=153
PRIMER_PAIR_PENALTY_3=0.4394
PRIMER_LEFT_3_PENALTY=0.080323
PRIMER_RIGHT_3_PENALTY=0.359050
PRIMER_LEFT_3_SEQUENCE=CAACGCAGAGTTTCTTCATTTG
PRIMER_RIGHT_3_SEQUENCE=TTTCTTCTACCCCTTGAAAACG
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=172,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=59.641
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=0.7633
PRIMER_LEFT_4_PENALTY=0.661199
PRIMER_RIGHT_4_PENALTY=0.102139
PRIMER_LEFT_4_SEQUENCE=CGGTATCAACGCAGAGTTTCTT
PRIMER_RIGHT_4_SEQUENCE=TTCTTCTACCCCTTGAAAACGA
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=171,22
PRIMER_LEFT_4_TM=60.661
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_02809 Ps_contig_mira-and-tgicl-ass_franssen_24943
SEQUENCE=CGACAGAGTATGCGKKGGARAGACCAAAACCCACTTTGAGAAAACATGGGGCCTCAAATGGCCTCTATTACTCTAACTATTGCATTGACAATAGTTTCCCTCACCTTGCCATCTGAAGCAAATAACTACATCTATTCATCTCCACCACCACCTAAACCTTACTACTACTACTCTCCACCACCACCAGTGCACTCACCTCCTCCACCTTACCATTATAGCTCACCACCACCACCACCAAAAAAGCAATATAAATACTCTTCTCCTCCACCACCGGTTTATAAATATAAATCTCCACCTCCACCTCCATATAAGTACCCATCACCACCACCTCCAGTTTACAAATACAAATCTCCTCCTCCACCAGTGTACAAGTATAAGTCACCACCACCTCCAGTTAAAAAACCATACAAGTACTCATCTCCCCCACCACCGGTTTATAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAAATCTCCTCCACCACCCGTTTACAAATACAAATCTCCTCCTCCACCAGTATACAAGTATAAGTCACCACCGCCTCCAGTTAAAAAACCATACAAATACACATCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCGGTGTACAAATATAAATCTCCTCCACCACCAATTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTACAAATATAACTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTCTATAAGTATAACTCTCCTCCACCACCAGTTTACAAGTATAATTCACCTCCTCCACCTGTGTATAAATACAAGTCACTACCACCTCCAGTTAAAAAACCATATAAGTACGCGTCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCAGTTTACAAATATAAATCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCTCCCCCACCACCGGTTTATAAATACAAGTCACCTCCTCCACCCATTTACAAGTATAATTCTCCCCCACCACCGGTTTATAAGTATAAGTCACCTCCTCCGCCAGTTTACAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCGCCAGTTTACAAATACAAATCACCTCCTCCCCCAGTGTACAAGTACAAGTCACCACCTCCTCCGATTAAAAAACCATACAAATATTCATCTCCACCACCACCAATATACAAGTATAAGTCACCTCCCCCACCGACTTACAAATATAAATCTCCTCCTCCACCTGTATATTCACCACCTTTACCACATTATATCTACTCTTCACCTCCTCCACCAGCTCATTCCCCTCCTCCACCACAATACATCTATGCATCACCTCCTCCTCCTTATCACTACTAGATAGTGGTTTACAATAGAATCCCATGGATTTTATGAATATATTGGAAATAAGCAGGAAGATGCATTTGGCAATAATACAAAGAAGAAAACAACGGAAGCAAAGTTTCTCATTTGTTTTGTTATGAATTATTATTATTCTCATTACTATTAGTGTGCTTTGTTTTTGAATTTTAGACAGCTGTAAGGCACTGTGTGTAGTAAATTATGAATGTATTTTGTTTTGGAACCTACTCGTTTTCAAGGGGTAGAGAAAATAAAATTGAAAAATTCTCCTTCACCRGT
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02810 Ps_contig_mira-and-tgicl-ass_franssen_24943
SEQUENCE=CGACAGAGTATGCGKKGGARAGACCAAAACCCACTTTGAGAAAACATGGGGCCTCAAATGGCCTCTATTACTCTAACTATTGCATTGACAATAGTTTCCCTCACCTTGCCATCTGAAGCAAATAACTACATCTATTCATCTCCACCACCACCTAAACCTTACTACTACTACTCTCCACCACCACCAGTGCACTCACCTCCTCCACCTTACCATTATAGCTCACCACCACCACCACCAAAAAAGCAATATAAATACTCTTCTCCTCCACCACCGGTTTATAAATATAAATCTCCACCTCCACCTCCATATAAGTACCCATCACCACCACCTCCAGTTTACAAATACAAATCTCCTCCTCCACCAGTGTACAAGTATAAGTCACCACCACCTCCAGTTAAAAAACCATACAAGTACTCATCTCCCCCACCACCGGTTTATAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAAATCTCCTCCACCACCCGTTTACAAATACAAATCTCCTCCTCCACCAGTATACAAGTATAAGTCACCACCGCCTCCAGTTAAAAAACCATACAAATACACATCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCGGTGTACAAATATAAATCTCCTCCACCACCAATTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTACAAATATAACTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTCTATAAGTATAACTCTCCTCCACCACCAGTTTACAAGTATAATTCACCTCCTCCACCTGTGTATAAATACAAGTCACTACCACCTCCAGTTAAAAAACCATATAAGTACGCGTCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCAGTTTACAAATATAAATCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCTCCCCCACCACCGGTTTATAAATACAAGTCACCTCCTCCACCCATTTACAAGTATAATTCTCCCCCACCACCGGTTTATAAGTATAAGTCACCTCCTCCGCCAGTTTACAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCGCCAGTTTACAAATACAAATCACCTCCTCCCCCAGTGTACAAGTACAAGTCACCACCTCCTCCGATTAAAAAACCATACAAATATTCATCTCCACCACCACCAATATACAAGTATAAGTCACCTCCCCCACCGACTTACAAATATAAATCTCCTCCTCCACCTGTATATTCACCACCTTTACCACATTATATCTACTCTTCACCTCCTCCACCAGCTCATTCCCCTCCTCCACCACAATACATCTATGCATCACCTCCTCCTCCTTATCACTACTAGATAGTGGTTTACAATAGAATCCCATGGATTTTATGAATATATTGGAAATAAGCAGGAAGATGCATTTGGCAATAATACAAAGAAGAAAACAACGGAAGCAAAGTTTCTCATTTGTTTTGTTATGAATTATTATTATTCTCATTACTATTAGTGTGCTTTGTTTTTGAATTTTAGACAGCTGTAAGGCACTGTGTGTAGTAAATTATGAATGTATTTTGTTTTGGAACCTACTCGTTTTCAAGGGGTAGAGAAAATAAAATTGAAAAATTCTCCTTCACCRGT
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02811 Ps_contig_mira-and-tgicl-ass_franssen_24943
SEQUENCE=CGACAGAGTATGCGKKGGARAGACCAAAACCCACTTTGAGAAAACATGGGGCCTCAAATGGCCTCTATTACTCTAACTATTGCATTGACAATAGTTTCCCTCACCTTGCCATCTGAAGCAAATAACTACATCTATTCATCTCCACCACCACCTAAACCTTACTACTACTACTCTCCACCACCACCAGTGCACTCACCTCCTCCACCTTACCATTATAGCTCACCACCACCACCACCAAAAAAGCAATATAAATACTCTTCTCCTCCACCACCGGTTTATAAATATAAATCTCCACCTCCACCTCCATATAAGTACCCATCACCACCACCTCCAGTTTACAAATACAAATCTCCTCCTCCACCAGTGTACAAGTATAAGTCACCACCACCTCCAGTTAAAAAACCATACAAGTACTCATCTCCCCCACCACCGGTTTATAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAAATCTCCTCCACCACCCGTTTACAAATACAAATCTCCTCCTCCACCAGTATACAAGTATAAGTCACCACCGCCTCCAGTTAAAAAACCATACAAATACACATCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCGGTGTACAAATATAAATCTCCTCCACCACCAATTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTACAAATATAACTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTCTATAAGTATAACTCTCCTCCACCACCAGTTTACAAGTATAATTCACCTCCTCCACCTGTGTATAAATACAAGTCACTACCACCTCCAGTTAAAAAACCATATAAGTACGCGTCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCAGTTTACAAATATAAATCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCTCCCCCACCACCGGTTTATAAATACAAGTCACCTCCTCCACCCATTTACAAGTATAATTCTCCCCCACCACCGGTTTATAAGTATAAGTCACCTCCTCCGCCAGTTTACAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCGCCAGTTTACAAATACAAATCACCTCCTCCCCCAGTGTACAAGTACAAGTCACCACCTCCTCCGATTAAAAAACCATACAAATATTCATCTCCACCACCACCAATATACAAGTATAAGTCACCTCCCCCACCGACTTACAAATATAAATCTCCTCCTCCACCTGTATATTCACCACCTTTACCACATTATATCTACTCTTCACCTCCTCCACCAGCTCATTCCCCTCCTCCACCACAATACATCTATGCATCACCTCCTCCTCCTTATCACTACTAGATAGTGGTTTACAATAGAATCCCATGGATTTTATGAATATATTGGAAATAAGCAGGAAGATGCATTTGGCAATAATACAAAGAAGAAAACAACGGAAGCAAAGTTTCTCATTTGTTTTGTTATGAATTATTATTATTCTCATTACTATTAGTGTGCTTTGTTTTTGAATTTTAGACAGCTGTAAGGCACTGTGTGTAGTAAATTATGAATGTATTTTGTTTTGGAACCTACTCGTTTTCAAGGGGTAGAGAAAATAAAATTGAAAAATTCTCCTTCACCRGT
TARGET=221,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02812 Ps_contig_mira-and-tgicl-ass_franssen_24943
SEQUENCE=CGACAGAGTATGCGKKGGARAGACCAAAACCCACTTTGAGAAAACATGGGGCCTCAAATGGCCTCTATTACTCTAACTATTGCATTGACAATAGTTTCCCTCACCTTGCCATCTGAAGCAAATAACTACATCTATTCATCTCCACCACCACCTAAACCTTACTACTACTACTCTCCACCACCACCAGTGCACTCACCTCCTCCACCTTACCATTATAGCTCACCACCACCACCACCAAAAAAGCAATATAAATACTCTTCTCCTCCACCACCGGTTTATAAATATAAATCTCCACCTCCACCTCCATATAAGTACCCATCACCACCACCTCCAGTTTACAAATACAAATCTCCTCCTCCACCAGTGTACAAGTATAAGTCACCACCACCTCCAGTTAAAAAACCATACAAGTACTCATCTCCCCCACCACCGGTTTATAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAAATCTCCTCCACCACCCGTTTACAAATACAAATCTCCTCCTCCACCAGTATACAAGTATAAGTCACCACCGCCTCCAGTTAAAAAACCATACAAATACACATCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCGGTGTACAAATATAAATCTCCTCCACCACCAATTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTACAAATATAACTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTCTATAAGTATAACTCTCCTCCACCACCAGTTTACAAGTATAATTCACCTCCTCCACCTGTGTATAAATACAAGTCACTACCACCTCCAGTTAAAAAACCATATAAGTACGCGTCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCAGTTTACAAATATAAATCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCTCCCCCACCACCGGTTTATAAATACAAGTCACCTCCTCCACCCATTTACAAGTATAATTCTCCCCCACCACCGGTTTATAAGTATAAGTCACCTCCTCCGCCAGTTTACAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCGCCAGTTTACAAATACAAATCACCTCCTCCCCCAGTGTACAAGTACAAGTCACCACCTCCTCCGATTAAAAAACCATACAAATATTCATCTCCACCACCACCAATATACAAGTATAAGTCACCTCCCCCACCGACTTACAAATATAAATCTCCTCCTCCACCTGTATATTCACCACCTTTACCACATTATATCTACTCTTCACCTCCTCCACCAGCTCATTCCCCTCCTCCACCACAATACATCTATGCATCACCTCCTCCTCCTTATCACTACTAGATAGTGGTTTACAATAGAATCCCATGGATTTTATGAATATATTGGAAATAAGCAGGAAGATGCATTTGGCAATAATACAAAGAAGAAAACAACGGAAGCAAAGTTTCTCATTTGTTTTGTTATGAATTATTATTATTCTCATTACTATTAGTGTGCTTTGTTTTTGAATTTTAGACAGCTGTAAGGCACTGTGTGTAGTAAATTATGAATGTATTTTGTTTTGGAACCTACTCGTTTTCAAGGGGTAGAGAAAATAAAATTGAAAAATTCTCCTTCACCRGT
TARGET=1318,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02813 Ps_contig_mira-and-tgicl-ass_franssen_24943
SEQUENCE=CGACAGAGTATGCGKKGGARAGACCAAAACCCACTTTGAGAAAACATGGGGCCTCAAATGGCCTCTATTACTCTAACTATTGCATTGACAATAGTTTCCCTCACCTTGCCATCTGAAGCAAATAACTACATCTATTCATCTCCACCACCACCTAAACCTTACTACTACTACTCTCCACCACCACCAGTGCACTCACCTCCTCCACCTTACCATTATAGCTCACCACCACCACCACCAAAAAAGCAATATAAATACTCTTCTCCTCCACCACCGGTTTATAAATATAAATCTCCACCTCCACCTCCATATAAGTACCCATCACCACCACCTCCAGTTTACAAATACAAATCTCCTCCTCCACCAGTGTACAAGTATAAGTCACCACCACCTCCAGTTAAAAAACCATACAAGTACTCATCTCCCCCACCACCGGTTTATAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAAATCTCCTCCACCACCCGTTTACAAATACAAATCTCCTCCTCCACCAGTATACAAGTATAAGTCACCACCGCCTCCAGTTAAAAAACCATACAAATACACATCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCGGTGTACAAATATAAATCTCCTCCACCACCAATTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTACAAATATAACTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTCTATAAGTATAACTCTCCTCCACCACCAGTTTACAAGTATAATTCACCTCCTCCACCTGTGTATAAATACAAGTCACTACCACCTCCAGTTAAAAAACCATATAAGTACGCGTCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCAGTTTACAAATATAAATCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCTCCCCCACCACCGGTTTATAAATACAAGTCACCTCCTCCACCCATTTACAAGTATAATTCTCCCCCACCACCGGTTTATAAGTATAAGTCACCTCCTCCGCCAGTTTACAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCGCCAGTTTACAAATACAAATCACCTCCTCCCCCAGTGTACAAGTACAAGTCACCACCTCCTCCGATTAAAAAACCATACAAATATTCATCTCCACCACCACCAATATACAAGTATAAGTCACCTCCCCCACCGACTTACAAATATAAATCTCCTCCTCCACCTGTATATTCACCACCTTTACCACATTATATCTACTCTTCACCTCCTCCACCAGCTCATTCCCCTCCTCCACCACAATACATCTATGCATCACCTCCTCCTCCTTATCACTACTAGATAGTGGTTTACAATAGAATCCCATGGATTTTATGAATATATTGGAAATAAGCAGGAAGATGCATTTGGCAATAATACAAAGAAGAAAACAACGGAAGCAAAGTTTCTCATTTGTTTTGTTATGAATTATTATTATTCTCATTACTATTAGTGTGCTTTGTTTTTGAATTTTAGACAGCTGTAAGGCACTGTGTGTAGTAAATTATGAATGTATTTTGTTTTGGAACCTACTCGTTTTCAAGGGGTAGAGAAAATAAAATTGAAAAATTCTCCTTCACCRGT
TARGET=1480,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02814 Ps_contig_mira-and-tgicl-ass_franssen_24943
SEQUENCE=CGACAGAGTATGCGKKGGARAGACCAAAACCCACTTTGAGAAAACATGGGGCCTCAAATGGCCTCTATTACTCTAACTATTGCATTGACAATAGTTTCCCTCACCTTGCCATCTGAAGCAAATAACTACATCTATTCATCTCCACCACCACCTAAACCTTACTACTACTACTCTCCACCACCACCAGTGCACTCACCTCCTCCACCTTACCATTATAGCTCACCACCACCACCACCAAAAAAGCAATATAAATACTCTTCTCCTCCACCACCGGTTTATAAATATAAATCTCCACCTCCACCTCCATATAAGTACCCATCACCACCACCTCCAGTTTACAAATACAAATCTCCTCCTCCACCAGTGTACAAGTATAAGTCACCACCACCTCCAGTTAAAAAACCATACAAGTACTCATCTCCCCCACCACCGGTTTATAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAAATCTCCTCCACCACCCGTTTACAAATACAAATCTCCTCCTCCACCAGTATACAAGTATAAGTCACCACCGCCTCCAGTTAAAAAACCATACAAATACACATCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCGGTGTACAAATATAAATCTCCTCCACCACCAATTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTACAAATATAACTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTCTATAAGTATAACTCTCCTCCACCACCAGTTTACAAGTATAATTCACCTCCTCCACCTGTGTATAAATACAAGTCACTACCACCTCCAGTTAAAAAACCATATAAGTACGCGTCTCCACCACCGCCAGTTTACAAGTACAATTCCCCTCCTCCACCAGTTTACAAATATAAATCTCCTCCACCACCAGTTTACAAGTATAAGTCACCTCCTCCACCAGTGTATAAATATAATTCTCCCCCACCACCGGTTTATAAATACAAGTCACCTCCTCCACCCATTTACAAGTATAATTCTCCCCCACCACCGGTTTATAAGTATAAGTCACCTCCTCCGCCAGTTTACAAGTACAATTCACCTCCTCCACCAGTGTACAAGTATAATTCTCCTCCACCGCCAGTTTACAAATACAAATCACCTCCTCCCCCAGTGTACAAGTACAAGTCACCACCTCCTCCGATTAAAAAACCATACAAATATTCATCTCCACCACCACCAATATACAAGTATAAGTCACCTCCCCCACCGACTTACAAATATAAATCTCCTCCTCCACCTGTATATTCACCACCTTTACCACATTATATCTACTCTTCACCTCCTCCACCAGCTCATTCCCCTCCTCCACCACAATACATCTATGCATCACCTCCTCCTCCTTATCACTACTAGATAGTGGTTTACAATAGAATCCCATGGATTTTATGAATATATTGGAAATAAGCAGGAAGATGCATTTGGCAATAATACAAAGAAGAAAACAACGGAAGCAAAGTTTCTCATTTGTTTTGTTATGAATTATTATTATTCTCATTACTATTAGTGTGCTTTGTTTTTGAATTTTAGACAGCTGTAAGGCACTGTGTGTAGTAAATTATGAATGTATTTTGTTTTGGAACCTACTCGTTTTCAAGGGGTAGAGAAAATAAAATTGAAAAATTCTCCTTCACCRGT
TARGET=1629,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02815 Ps_contig_mira-and-tgicl-ass_franssen_24946
SEQUENCE=GTTGGGTGAAACAGATCCTAATTCCAAATTCTATATTTCTAAACCTAATTTTCTCTCCTCGGTCTCGCCGTTGATCTGTTTCAATTTTTTCTGCATTCTTTGTCTTATAATCCGAAATCTTGATTCACAGAGGGGAAGTCGAGAAACAGATTGTTCTCCGATTGTTAAACAACAGAAGGTGTGAATGAAGTAATGGAGTCAAAAGGTGGTAAGAAGAAGTCTAGTAGTAGTAGTAAATGCTCTTTATACGAAGCTCCTCTCGATTACATCATTGAAGACGTTAGACCACACGGTGGAGTAGAAAAATTCAAATCTGCTGATTACTCAAACTGCACCCGCAAACCCTCCTGAGATTTTCTGGGTTCTGATAGATTTAGGTTTTTCATCTTTCATACTGCAATATTATTCTAGTTTCTTCTGCTCCTTTTTTCCTCCTACTGGTTGCTTTTCTTCTGCTCCTGTTCTGTTTCTCGACAAAAAACAAGAATTGTTTTCTCTTTTGAATCAAGATGGCTATGACAAACTCAGCTATTGGTTTTGAAGGCTACGAAAAGAGGCTTGAGATGACTTTTTTTGAGAGTGGTGTTTTCTCTGATCCTGCTGGATTAGGTCTACGAGCATTGTCTAAAGACCAATTGGATGAAATTCTGAACCCAGCCGAGTGCACTATTGTTGACTCATTGTCTAATGATTATGTTGATTCATATGTTCTTTCTGAATCAAGCTTGTTTGTGTATGCTTACAAAGTTATCATCAAAACCTGTGGGACTACAAAGTTGCTTCTGTCTATCCCTGCAATTCTTAAGCTGGCTGATGGTCTTAACATTGCTGTGAAATCGGTGAGATACACTCGCGGTAGCTTCATCTTTCCCGGTGCTCAATCATTCCCTCATCGAAGCTTTTCTGAGGAAGTTGATGTTCTTGACAGCTATTTTGGCAAACTTGGTTCTGGTAGCCAAGCTTATATGATGGGTGATAGTGATAAGTCACAAATTTGGCACATTTACTCTGCCAGTGCTAAGGCAGAAGCTTCACCGGAAGCTGTATATGGCCTTGAAATGTGCATGACTGGTTTAGATAAGGAAAAAGCATCTGTGTTTTTCAAGACAAGCACATCTTCAGCAGCTTTGATGACTAAGAATTCTGGAATCAGGAAGATCCTTCCAAAATCTGACATATGTGACTTTGAATTTGATCCATGTGGGTATTCAATGAATGGAATAGAAGGGAATGCCATATCGACTATCCATGTAACCCCTGAAGATGGATTCAGTTATGCAAGCTTTGAAGCTGTGGGTTATGAGTATGAAGAGAAATCTCTGAATGAAGTTGTTGAAAGGGTTTTAGCATGTTTCTATCCAGCTGAGTTTTCTATTGCTTTGCACATCGATATGAATGGTGAAAAACTTGACAAATTTCCTCTAGACGTTAAAGGATACTACAGTGGGAAGAGGAGCAATGAAGTTGTTGGAGAAGGTGGTGCTGTAGTTTACCGTTCCTTTGTTCGAAATGATGGCTGCGCATCTCCAAAGTCTACTCTGAAATGTTGCTGGAGTGAGGATGAGAGCGAGGAGGAAGAAGGGAAGGAGATATAGTACTCTGGTTTTAGTTTCTTATGTTCTGTTCCTGTCTATGTTGTCTGTTTTCAGTGTTAATTCCTAAATAAACTTTTGCTTTTGTTATGAAAGCGATACAATAAACGTGTAATGTTGTTGTGAAAGCGATACAATAATAATACGTATTATGTTATAAAATACTTTATTTGTAATTTTCATGTGGATTTTAAGTTTTGACATTAATTTTCTT
TARGET=222,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4202
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.360320
PRIMER_LEFT_SEQUENCE=GAAATCTTGATTCACAGAGGGG
PRIMER_RIGHT_SEQUENCE=GATGTAATCGAGAGGAGCTTCG
PRIMER_LEFT=113,22
PRIMER_RIGHT=269,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=60.360
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.4202
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=0.360320
PRIMER_LEFT_1_SEQUENCE=AAATCTTGATTCACAGAGGGGA
PRIMER_RIGHT_1_SEQUENCE=GATGTAATCGAGAGGAGCTTCG
PRIMER_LEFT_1=114,22
PRIMER_RIGHT_1=269,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=60.360
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.4202
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.360320
PRIMER_LEFT_2_SEQUENCE=AATCTTGATTCACAGAGGGGAA
PRIMER_RIGHT_2_SEQUENCE=GATGTAATCGAGAGGAGCTTCG
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=269,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=60.360
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.4705
PRIMER_LEFT_3_PENALTY=0.110131
PRIMER_RIGHT_3_PENALTY=0.360320
PRIMER_LEFT_3_SEQUENCE=CTTGATTCACAGAGGGGAAGTC
PRIMER_RIGHT_3_SEQUENCE=GATGTAATCGAGAGGAGCTTCG
PRIMER_LEFT_3=118,22
PRIMER_RIGHT_3=269,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=60.360
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=0.4705
PRIMER_LEFT_4_PENALTY=0.110131
PRIMER_RIGHT_4_PENALTY=0.360320
PRIMER_LEFT_4_SEQUENCE=TCTTGATTCACAGAGGGGAAGT
PRIMER_RIGHT_4_SEQUENCE=GATGTAATCGAGAGGAGCTTCG
PRIMER_LEFT_4=117,22
PRIMER_RIGHT_4=269,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=60.360
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_02816 Ps_contig_mira-and-tgicl-ass_franssen_24953
SEQUENCE=ACACTTGTCAAAAGTATACATCATATTATAATTTATTACGATCACATAACAAACAAACAGAAACAATAAACCTAAAAATGAATTGAACTAGAGAAAAACAATTTCGTAGAAACGACTCTTTTAAGGAAAAAAGAAATTACAACTAAAAATGATGACAACGACGACAATAACAACAACACCAACAACATAAAAAGTAGAAGATTGTGATACAATGCAAAAGCAACCAATGATAAAAAATTAAAGAAATCTAACACAACCGAAGAGCTAGAAACTATAAGTCCAAACAAATCAAGGGTATCATCGACCCTTCTTACAAAGTACAATTAAAAAGGCGTCTAAAACATGAAACGAGTAAAATTGTTTCATGGCACTACTTGCATCTTCTCACATTCCTCTCGCACTTCTTGAAAAAGAACACTAGATAAATATCTTTCACCGAAACTTGGAAATATCACCACAATAAGCTTTCCCTCATTTTCAGGCCTCTTTGCAAGCTGCAATGCGGCTGCAGCAGCACAACCCGAAGACATTCCCACCAACAGGCCTTCCTGAAGTGCTATTGTCTTTGAAGTTTTGATTGATTCTTCACCTGATATCGCTATAACTTCATCCATGACTTTTCTATCCAAATTTTTGGGCACAAAACCAGCCCCAATACCCTGAATTATGTGAGGATTTGCTGTTCCACCGCTAAGTACGTTGCTTTCGAGAGGCTCTACACCAACAACCTTAATGTTTGGGTTTTGTTCTTTTAGAAATCGAGCTGTTCCAGTAAGGGTTCCACCAGTTCCAATTCCTGAAACTAAAATATCTACCTTACCTCTTGTATCTTTCCATATCTCTGGCCCAGTGCTGTCGTAATGTGCTTGAGGATTTGAAGGATTATCATATTGTTGAAGCATGAATGCATTAGGTGTATTTTTCACAATTTCTTCAGCTTTTTGAATTGCTCCAGGTATAGACTTGGAATGTTCAGTCAAAACAAGTTCAGCTCCAAATGCTCTCATAAGAATTCTTCTTTCTAAACTCATTTGAACAGGCATTGTCAAAATGAGTTTATATCCTTTAGTAGCTGCTATATAGGCAAGACCAATTCCTGTGTTACCACCGGTAGGTTCCACCAAAATACTCTTTCCGGGTGTAATGAGTGTAATGAATCCTTTTCCTCTCAGC
TARGET=48,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02817 Ps_contig_mira-and-tgicl-ass_franssen_24972
SEQUENCE=TATCAACGCAGAGTACTCGGGGWTGATTTATCTCAATCGTGTTCCATTCTGTGATCCCAAATCAAAGCGAGTTCGAAAAATGTCGTATTTTTCAAGGGGTGGTGGTGGAAACAATGACGAAAGCGTTGATGATTTCGACGAATACGATCCAACACCATACGGTGGAGGTTACGACATTCACCTAACCTACGGTCGTCCAATTCCACCCTCTGAAGAAACATGTTACCCATCAGGTTCAGCCGATGATGATTTCGATTACGATCGTCCTCAATTTTCGTCTCAATCTGAACCTTCTGCTTATGGCGATGAAGCTCTTGCTACTGAGTATAGTAGCTATTCTCGACCTAAACCTAGACCTGCTCCTGCTGGTTTTAACCCTGGATCTGCTGGTGGTTATGGTGGCGGGAGAAAGCAGGGATCTGGGTATGGTGGTGGGAGGCAGGAATCTGAATTTGGATCTGGGTATGGTGATGGGGCCCAGGAATCTGAGTATGGATCTGGTTATGGTGGTGGAAGAAAGCAAGAATCTGGATATGGTAGAAATGATGAGGAATCTGAATTTGGGTCTGGTTATGGTAGAAAGAATGAGGGATCTGAATATGGATCTGGGTATGGTGGGAGGAAGCAAGAATATGGGAGGAGTGAGGAGGAGAGTCAAGGTTATGGGAGGAAGACTGAGTATGAGAGTGGCGGATCGGAATATGGATCGGGGTATGGTGGGAGGAAGAAGACTGGTTATGGGGAGGAAGAGGGATATGGAGGAGGAAGGAGTGAGTATGAAGCAAAACCTAGCTATGGAAGGAGTGATGATGAGGAGAAACCGAGCTATGGTTATGAGCGTCCTGAGAGGCGTCGTGATGATGATGAGGAGAAACCGAGCTATGGTTATGAGCGTCCTGAGAGGCGTCGTGATGATGATGATGATGATGAGAAACCCAGCTATGGTCGCAAGAAATATGGAGGAGGTGAAGATAACTCTGATGATAATGATGATGATGATGGGAGGAAGAAGCATCACCACAAGAGTCACCATCGCAAGAGCTATGATGATGACGACGAGTAATCAGAAAGACCGAAGTTGTTGTGTGCGTGGAAGTCATCCCATAGTTACTTATTAAATAAAGGGTTGACACATATCATACTTTCTTCTTTACAGTTTGAACTATACTATGAATTCTTGAGCTTTCAGAACTATTGTTCTAAACTAAATGTATTGTTGGTTTGGCTTTTGACTTGTTATAATATCCCTAATGTTTGAGTTGTTGTGTTACACTACATGTATGGTAAAGTTTGAATTTGTTTACACCTGTATGAATTTGGATAGAATATTATGTCAAAAAGTTCATCCATGTAGCAGATCTTACTTAACGGNTTTGGATATGATTTTAGG
TARGET=856,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02818 Ps_contig_mira-and-tgicl-ass_franssen_24972
SEQUENCE=TATCAACGCAGAGTACTCGGGGWTGATTTATCTCAATCGTGTTCCATTCTGTGATCCCAAATCAAAGCGAGTTCGAAAAATGTCGTATTTTTCAAGGGGTGGTGGTGGAAACAATGACGAAAGCGTTGATGATTTCGACGAATACGATCCAACACCATACGGTGGAGGTTACGACATTCACCTAACCTACGGTCGTCCAATTCCACCCTCTGAAGAAACATGTTACCCATCAGGTTCAGCCGATGATGATTTCGATTACGATCGTCCTCAATTTTCGTCTCAATCTGAACCTTCTGCTTATGGCGATGAAGCTCTTGCTACTGAGTATAGTAGCTATTCTCGACCTAAACCTAGACCTGCTCCTGCTGGTTTTAACCCTGGATCTGCTGGTGGTTATGGTGGCGGGAGAAAGCAGGGATCTGGGTATGGTGGTGGGAGGCAGGAATCTGAATTTGGATCTGGGTATGGTGATGGGGCCCAGGAATCTGAGTATGGATCTGGTTATGGTGGTGGAAGAAAGCAAGAATCTGGATATGGTAGAAATGATGAGGAATCTGAATTTGGGTCTGGTTATGGTAGAAAGAATGAGGGATCTGAATATGGATCTGGGTATGGTGGGAGGAAGCAAGAATATGGGAGGAGTGAGGAGGAGAGTCAAGGTTATGGGAGGAAGACTGAGTATGAGAGTGGCGGATCGGAATATGGATCGGGGTATGGTGGGAGGAAGAAGACTGGTTATGGGGAGGAAGAGGGATATGGAGGAGGAAGGAGTGAGTATGAAGCAAAACCTAGCTATGGAAGGAGTGATGATGAGGAGAAACCGAGCTATGGTTATGAGCGTCCTGAGAGGCGTCGTGATGATGATGAGGAGAAACCGAGCTATGGTTATGAGCGTCCTGAGAGGCGTCGTGATGATGATGATGATGATGAGAAACCCAGCTATGGTCGCAAGAAATATGGAGGAGGTGAAGATAACTCTGATGATAATGATGATGATGATGGGAGGAAGAAGCATCACCACAAGAGTCACCATCGCAAGAGCTATGATGATGACGACGAGTAATCAGAAAGACCGAAGTTGTTGTGTGCGTGGAAGTCATCCCATAGTTACTTATTAAATAAAGGGTTGACACATATCATACTTTCTTCTTTACAGTTTGAACTATACTATGAATTCTTGAGCTTTCAGAACTATTGTTCTAAACTAAATGTATTGTTGGTTTGGCTTTTGACTTGTTATAATATCCCTAATGTTTGAGTTGTTGTGTTACACTACATGTATGGTAAAGTTTGAATTTGTTTACACCTGTATGAATTTGGATAGAATATTATGTCAAAAAGTTCATCCATGTAGCAGATCTTACTTAACGGNTTTGGATATGATTTTAGG
TARGET=910,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02819 Ps_contig_mira-and-tgicl-ass_franssen_24972
SEQUENCE=TATCAACGCAGAGTACTCGGGGWTGATTTATCTCAATCGTGTTCCATTCTGTGATCCCAAATCAAAGCGAGTTCGAAAAATGTCGTATTTTTCAAGGGGTGGTGGTGGAAACAATGACGAAAGCGTTGATGATTTCGACGAATACGATCCAACACCATACGGTGGAGGTTACGACATTCACCTAACCTACGGTCGTCCAATTCCACCCTCTGAAGAAACATGTTACCCATCAGGTTCAGCCGATGATGATTTCGATTACGATCGTCCTCAATTTTCGTCTCAATCTGAACCTTCTGCTTATGGCGATGAAGCTCTTGCTACTGAGTATAGTAGCTATTCTCGACCTAAACCTAGACCTGCTCCTGCTGGTTTTAACCCTGGATCTGCTGGTGGTTATGGTGGCGGGAGAAAGCAGGGATCTGGGTATGGTGGTGGGAGGCAGGAATCTGAATTTGGATCTGGGTATGGTGATGGGGCCCAGGAATCTGAGTATGGATCTGGTTATGGTGGTGGAAGAAAGCAAGAATCTGGATATGGTAGAAATGATGAGGAATCTGAATTTGGGTCTGGTTATGGTAGAAAGAATGAGGGATCTGAATATGGATCTGGGTATGGTGGGAGGAAGCAAGAATATGGGAGGAGTGAGGAGGAGAGTCAAGGTTATGGGAGGAAGACTGAGTATGAGAGTGGCGGATCGGAATATGGATCGGGGTATGGTGGGAGGAAGAAGACTGGTTATGGGGAGGAAGAGGGATATGGAGGAGGAAGGAGTGAGTATGAAGCAAAACCTAGCTATGGAAGGAGTGATGATGAGGAGAAACCGAGCTATGGTTATGAGCGTCCTGAGAGGCGTCGTGATGATGATGAGGAGAAACCGAGCTATGGTTATGAGCGTCCTGAGAGGCGTCGTGATGATGATGATGATGATGAGAAACCCAGCTATGGTCGCAAGAAATATGGAGGAGGTGAAGATAACTCTGATGATAATGATGATGATGATGGGAGGAAGAAGCATCACCACAAGAGTCACCATCGCAAGAGCTATGATGATGACGACGAGTAATCAGAAAGACCGAAGTTGTTGTGTGCGTGGAAGTCATCCCATAGTTACTTATTAAATAAAGGGTTGACACATATCATACTTTCTTCTTTACAGTTTGAACTATACTATGAATTCTTGAGCTTTCAGAACTATTGTTCTAAACTAAATGTATTGTTGGTTTGGCTTTTGACTTGTTATAATATCCCTAATGTTTGAGTTGTTGTGTTACACTACATGTATGGTAAAGTTTGAATTTGTTTACACCTGTATGAATTTGGATAGAATATTATGTCAAAAAGTTCATCCATGTAGCAGATCTTACTTAACGGNTTTGGATATGATTTTAGG
TARGET=987,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02820 Ps_contig_mira-and-tgicl-ass_franssen_24974
SEQUENCE=GCCCAAGACCGGAAATATCACCGGTCAGATCTTTATCTTCGAAAGACATCTTTGCTAAAGCTCTACTAGCATCCTTGCCAGCAAATAAAGCATAAGGACCGCCGGGTCCATAGAACATCCTGCTCTGGGAGACATCATAGATCTGGCCCTTAATGGCCATGAGCAAGGGCTTATCGTGATCGTTGCCATCGTAGGCCTTGAGCTCTTCTTCGGTGATTTCACCTAGCTGAACGGGGGGTCGGAGAGGGGGAACCTCTGCTTGGATGTGGCGGGTTTGGTGGTGACGGTCGGAGGAGGAGGAGCCAAAGAGACCGGAAACGACGTAGAAAAGAGCAAATAGAATCGCCAATCCAGTGAAGAAAGTGGTCGGTGATAGACCGGTGAAGGAGAAGATGAGCTCCTTTTGGGCCTCCCACCACAGCTGGAAAGTGCCCATCACTCGGTGTTAGAGAGAGAGAGAGGAACAACAAAATGTAGACAGAGAAACGTTCCCGCGTA
TARGET=290,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0569
PRIMER_LEFT_PENALTY=0.018835
PRIMER_RIGHT_PENALTY=0.038090
PRIMER_LEFT_SEQUENCE=CTTGAGCTCTTCTTCGGTGATT
PRIMER_RIGHT_SEQUENCE=ATCACCGACCACTTTCTTCACT
PRIMER_LEFT=196,22
PRIMER_RIGHT=373,22
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.2919
PRIMER_LEFT_1_PENALTY=0.018835
PRIMER_RIGHT_1_PENALTY=0.273036
PRIMER_LEFT_1_SEQUENCE=CTTGAGCTCTTCTTCGGTGATT
PRIMER_RIGHT_1_SEQUENCE=GATTGGCGATTCTATTTGCTCT
PRIMER_LEFT_1=196,22
PRIMER_RIGHT_1=350,22
PRIMER_LEFT_1_TM=60.019
PRIMER_RIGHT_1_TM=59.727
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=155
PRIMER_PAIR_PENALTY_2=0.3031
PRIMER_LEFT_2_PENALTY=0.265053
PRIMER_RIGHT_2_PENALTY=0.038090
PRIMER_LEFT_2_SEQUENCE=ATAGAACATCCTGCTCTGGGAG
PRIMER_RIGHT_2_SEQUENCE=ATCACCGACCACTTTCTTCACT
PRIMER_LEFT_2=109,22
PRIMER_RIGHT_2=373,22
PRIMER_LEFT_2_TM=59.735
PRIMER_RIGHT_2_TM=60.038
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.3060
PRIMER_LEFT_3_PENALTY=0.018835
PRIMER_RIGHT_3_PENALTY=0.287182
PRIMER_LEFT_3_SEQUENCE=CTTGAGCTCTTCTTCGGTGATT
PRIMER_RIGHT_3_SEQUENCE=TCTTCTCCTTCACCGGTCTATC
PRIMER_LEFT_3=196,22
PRIMER_RIGHT_3=392,22
PRIMER_LEFT_3_TM=60.019
PRIMER_RIGHT_3_TM=59.713
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=0.3508
PRIMER_LEFT_4_PENALTY=0.018835
PRIMER_RIGHT_4_PENALTY=0.331942
PRIMER_LEFT_4_SEQUENCE=CTTGAGCTCTTCTTCGGTGATT
PRIMER_RIGHT_4_SEQUENCE=ACTGGATTGGCGATTCTATTTG
PRIMER_LEFT_4=196,22
PRIMER_RIGHT_4=354,22
PRIMER_LEFT_4_TM=60.019
PRIMER_RIGHT_4_TM=60.332
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_02821 Ps_contig_mira-and-tgicl-ass_franssen_24974
SEQUENCE=GCCCAAGACCGGAAATATCACCGGTCAGATCTTTATCTTCGAAAGACATCTTTGCTAAAGCTCTACTAGCATCCTTGCCAGCAAATAAAGCATAAGGACCGCCGGGTCCATAGAACATCCTGCTCTGGGAGACATCATAGATCTGGCCCTTAATGGCCATGAGCAAGGGCTTATCGTGATCGTTGCCATCGTAGGCCTTGAGCTCTTCTTCGGTGATTTCACCTAGCTGAACGGGGGGTCGGAGAGGGGGAACCTCTGCTTGGATGTGGCGGGTTTGGTGGTGACGGTCGGAGGAGGAGGAGCCAAAGAGACCGGAAACGACGTAGAAAAGAGCAAATAGAATCGCCAATCCAGTGAAGAAAGTGGTCGGTGATAGACCGGTGAAGGAGAAGATGAGCTCCTTTTGGGCCTCCCACCACAGCTGGAAAGTGCCCATCACTCGGTGTTAGAGAGAGAGAGAGGAACAACAAAATGTAGACAGAGAAACGTTCCCGCGTA
TARGET=448,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0784
PRIMER_LEFT_PENALTY=0.018835
PRIMER_RIGHT_PENALTY=2.059538
PRIMER_LEFT_SEQUENCE=CTTGAGCTCTTCTTCGGTGATT
PRIMER_RIGHT_SEQUENCE=GGGAACGTTTCTCTGTCTACATTT
PRIMER_LEFT=196,22
PRIMER_RIGHT=492,24
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=59.940
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=2.0976
PRIMER_LEFT_1_PENALTY=0.038090
PRIMER_RIGHT_1_PENALTY=2.059538
PRIMER_LEFT_1_SEQUENCE=AGTGAAGAAAGTGGTCGGTGAT
PRIMER_RIGHT_1_SEQUENCE=GGGAACGTTTCTCTGTCTACATTT
PRIMER_LEFT_1=352,22
PRIMER_RIGHT_1=492,24
PRIMER_LEFT_1_TM=60.038
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=2.1678
PRIMER_LEFT_2_PENALTY=0.018835
PRIMER_RIGHT_2_PENALTY=2.149011
PRIMER_LEFT_2_SEQUENCE=CTTGAGCTCTTCTTCGGTGATT
PRIMER_RIGHT_2_SEQUENCE=CGGGAACGTTTCTCTGTCTAC
PRIMER_LEFT_2=196,22
PRIMER_RIGHT_2=493,21
PRIMER_LEFT_2_TM=60.019
PRIMER_RIGHT_2_TM=58.851
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=2.1871
PRIMER_LEFT_3_PENALTY=0.038090
PRIMER_RIGHT_3_PENALTY=2.149011
PRIMER_LEFT_3_SEQUENCE=AGTGAAGAAAGTGGTCGGTGAT
PRIMER_RIGHT_3_SEQUENCE=CGGGAACGTTTCTCTGTCTAC
PRIMER_LEFT_3=352,22
PRIMER_RIGHT_3=493,21
PRIMER_LEFT_3_TM=60.038
PRIMER_RIGHT_3_TM=58.851
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=142
PRIMER_PAIR_PENALTY_4=2.3122
PRIMER_LEFT_4_PENALTY=0.018835
PRIMER_RIGHT_4_PENALTY=2.293368
PRIMER_LEFT_4_SEQUENCE=CTTGAGCTCTTCTTCGGTGATT
PRIMER_RIGHT_4_SEQUENCE=GGGAACGTTTCTCTGTCTACATT
PRIMER_LEFT_4=196,22
PRIMER_RIGHT_4=492,23
PRIMER_LEFT_4_TM=60.019
PRIMER_RIGHT_4_TM=58.707
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_02822 Ps_contig_mira-and-tgicl-ass_franssen_24975
SEQUENCE=ATCAACGCAGAGTACGCGGGATCTTCCTCCATTTTCTCTCAGTTCTTTTCTCCTTTCATCTTCTCTCTACTAACTCCTTAACAATGTCAGCTTACACAAACCAGGATCCTCGTCTTCACGGCATCAAAACTAAAATCCGTGTCGTCCCCAACTTTCCCAAATCCGGTATCATGTTCCAAGACATTACCACTCTGTTACTGGATCCCAAAGCTTTCAAAGACACCATCGACTTGTTCGTTGAACGATACAAAGGCAAAAACATTTCTGTTGTTGCAGGAATCGAAGCTCGTGGTTTCATTTTTGGTCCTCCCATTGCCCTTGCAATAGGAGCTAAGTTTGTACCATTGAGGAAACCAAAGAAGTTGCCTGGCAAAGTTATATCTCAAGAATATATTCTGGAATATGGAAGCGATTGTCTTGAAATGCATGTTGGAGCTGTTGAGGCTGGTGAACGTGCTATTGTTGTGGATGATTTGATTGCCACCGGTGGAACACTCTGTGCCGCAATGGATTTACTTGAACGTGTTGGAGCAGAGGTGGTGGAGTGCGCATGTGTAATTGAATTGCCAGAACTAAAGGGTCGTGAACGATTGAATGGGAAGGCATTGTACGTGTTGGTGGAATACTTGGATGATATATGAGGTGCATGGACCCATAGAAATCAAAATTGTGGATTTTGGATATGTCATTTTGTGGACACACTTTAGTCCCATCCTAGCTAGCTTGTAGTATGAACAATCAAATCAAATTAATTAATTAATCAATTTGCTTCTCACTCTTTTCTCTGACATCATCACAAGAATGATAACTGGGTCCAGGACAGAGGTGCCTTAGGATCTATCTTTGTTAATGTTAATGTCATGTAGGTTGTTAGATACACATAATTA
TARGET=747,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0063
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=TGAATTGCCAGAACTAAAGGGT
PRIMER_RIGHT_SEQUENCE=CTCTGTCCTGGACCCAGTTATC
PRIMER_LEFT=559,22
PRIMER_RIGHT=824,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.0387
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=CCAGAACTAAAGGGTCGTGAAC
PRIMER_RIGHT_1_SEQUENCE=CTCTGTCCTGGACCCAGTTATC
PRIMER_LEFT_1=566,22
PRIMER_RIGHT_1=824,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.1741
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.173354
PRIMER_LEFT_2_SEQUENCE=TGAATTGCCAGAACTAAAGGGT
PRIMER_RIGHT_2_SEQUENCE=TCCTGGACCCAGTTATCATTCT
PRIMER_LEFT_2=559,22
PRIMER_RIGHT_2=819,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=59.827
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.2065
PRIMER_LEFT_3_PENALTY=0.033137
PRIMER_RIGHT_3_PENALTY=0.173354
PRIMER_LEFT_3_SEQUENCE=CCAGAACTAAAGGGTCGTGAAC
PRIMER_RIGHT_3_SEQUENCE=TCCTGGACCCAGTTATCATTCT
PRIMER_LEFT_3=566,22
PRIMER_RIGHT_3=819,22
PRIMER_LEFT_3_TM=60.033
PRIMER_RIGHT_3_TM=59.827
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.2329
PRIMER_LEFT_4_PENALTY=0.227356
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=TGTTGGTGGAATACTTGGATGA
PRIMER_RIGHT_4_SEQUENCE=CTCTGTCCTGGACCCAGTTATC
PRIMER_LEFT_4=612,22
PRIMER_RIGHT_4=824,22
PRIMER_LEFT_4_TM=60.227
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_02823 Ps_contig_mira-and-tgicl-ass_franssen_24984
SEQUENCE=AGAGACCTGAAAATAGAGGGAAAGSYTATTGTTGGTTGTATTTCCAAGCTTTGGCGAAAGGTATTTGTCCAGTTCTCTTTTCCAAGAAGTTCGAGAAGAATGTGAAAAGATGGAAGCAGTGCCATGAAACCATTTTAATCCTTTCATGTTTAAAACACATTTTCAAATGCAGTTCTTGATCATCTCACATGGAAACAAACACTATTAACATGTACTTTATAATTAATTAATATGGTGGATATATAACCTTAMAGGTTGTATGTTAGG
TARGET=220,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02824 Ps_contig_mira-and-tgicl-ass_franssen_24994
SEQUENCE=GTTTTTGGAAAAGGACCTATTGGTTTCATTGCTACCTTCTAACCAGTGACATAATTATATATGAAGTGAAACCCTTTTAGCTCAAAATCTTCTCTATCATGTATAAGTCTAAATATCACTCACTCCATGTTAAAATTGTTTATATTTTTATATGTATCCGCAGAAATTAAGAACTGCTATGTATGTATGTACCTCTGTGTTGTATGTCTTCTCGGTAATACCATATATAATCCTGATCCATATAATATAGTGTTGTCTTA
TARGET=178,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.7012
PRIMER_LEFT_PENALTY=0.623595
PRIMER_RIGHT_PENALTY=2.077587
PRIMER_LEFT_SEQUENCE=AAGGACCTATTGGTTTCATTGC
PRIMER_RIGHT_SEQUENCE=CCGAGAAGACATACAACACAGAG
PRIMER_LEFT=10,22
PRIMER_RIGHT=214,23
PRIMER_LEFT_TM=59.376
PRIMER_RIGHT_TM=58.922
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=2.7012
PRIMER_LEFT_1_PENALTY=0.623595
PRIMER_RIGHT_1_PENALTY=2.077587
PRIMER_LEFT_1_SEQUENCE=AAGGACCTATTGGTTTCATTGC
PRIMER_RIGHT_1_SEQUENCE=CGAGAAGACATACAACACAGAGG
PRIMER_LEFT_1=10,22
PRIMER_RIGHT_1=213,23
PRIMER_LEFT_1_TM=59.376
PRIMER_RIGHT_1_TM=58.922
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=2.7271
PRIMER_LEFT_2_PENALTY=0.649547
PRIMER_RIGHT_2_PENALTY=2.077587
PRIMER_LEFT_2_SEQUENCE=GGAAAAGGACCTATTGGTTTCA
PRIMER_RIGHT_2_SEQUENCE=CCGAGAAGACATACAACACAGAG
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=214,23
PRIMER_LEFT_2_TM=59.350
PRIMER_RIGHT_2_TM=58.922
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=2.7271
PRIMER_LEFT_3_PENALTY=0.649547
PRIMER_RIGHT_3_PENALTY=2.077587
PRIMER_LEFT_3_SEQUENCE=TGGAAAAGGACCTATTGGTTTC
PRIMER_RIGHT_3_SEQUENCE=CCGAGAAGACATACAACACAGAG
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=214,23
PRIMER_LEFT_3_TM=59.350
PRIMER_RIGHT_3_TM=58.922
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=2.7271
PRIMER_LEFT_4_PENALTY=0.649547
PRIMER_RIGHT_4_PENALTY=2.077587
PRIMER_LEFT_4_SEQUENCE=GGAAAAGGACCTATTGGTTTCA
PRIMER_RIGHT_4_SEQUENCE=CGAGAAGACATACAACACAGAGG
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=213,23
PRIMER_LEFT_4_TM=59.350
PRIMER_RIGHT_4_TM=58.922
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_02825 Ps_contig_mira-and-tgicl-ass_franssen_24997
SEQUENCE=TCGGGGTACTATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCAAACCCCCAAAACATAGGGAAACAACCCTCCACTCTATCTTCATCTCNCTCAAACCAACCAAGTTCACAACCAAGAACAAACAAAATTGCTGCAACAAGAAGAACCAAAACCGATGCAGCAACCGAT
TARGET=24,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5926
PRIMER_LEFT_PENALTY=0.547508
PRIMER_RIGHT_PENALTY=0.045112
PRIMER_LEFT_SEQUENCE=TCGGGGTACTATTGATCTCACA
PRIMER_RIGHT_SEQUENCE=TTGTTCTTGGTTGTGAACTTGG
PRIMER_LEFT=0,22
PRIMER_RIGHT=162,22
PRIMER_LEFT_TM=59.452
PRIMER_RIGHT_TM=60.045
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.5926
PRIMER_LEFT_1_PENALTY=0.547508
PRIMER_RIGHT_1_PENALTY=0.045112
PRIMER_LEFT_1_SEQUENCE=TCGGGGTACTATTGATCTCACA
PRIMER_RIGHT_1_SEQUENCE=TTCTTGGTTGTGAACTTGGTTG
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=159,22
PRIMER_LEFT_1_TM=59.452
PRIMER_RIGHT_1_TM=60.045
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.6338
PRIMER_LEFT_2_PENALTY=0.547508
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=TCGGGGTACTATTGATCTCACA
PRIMER_RIGHT_2_SEQUENCE=GTTGTGAACTTGGTTGGTTTGA
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=153,22
PRIMER_LEFT_2_TM=59.452
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=0.9322
PRIMER_LEFT_3_PENALTY=0.547508
PRIMER_RIGHT_3_PENALTY=0.384647
PRIMER_LEFT_3_SEQUENCE=TCGGGGTACTATTGATCTCACA
PRIMER_RIGHT_3_SEQUENCE=CATCGGTTTTGGTTCTTCTTGT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=198,22
PRIMER_LEFT_3_TM=59.452
PRIMER_RIGHT_3_TM=60.385
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=199
PRIMER_PAIR_PENALTY_4=0.9892
PRIMER_LEFT_4_PENALTY=0.547508
PRIMER_RIGHT_4_PENALTY=0.441731
PRIMER_LEFT_4_SEQUENCE=TCGGGGTACTATTGATCTCACA
PRIMER_RIGHT_4_SEQUENCE=GAACTTGGTTGGTTTGAGNGAG
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=148,22
PRIMER_LEFT_4_TM=59.452
PRIMER_RIGHT_4_TM=60.442
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.0250
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_02826 Ps_contig_mira-and-tgicl-ass_franssen_25007
SEQUENCE=CGGTTACAACTTTAATTTCAAACTTTACCCTAACGTCAATACACCACCAAAACTTACCTAATACCTTCCAAACTCCAATTATTATTATTAGTTGCAGCAGCTTCTTCAGTTGCAGTACTTCCTGTGGCGACGGTYTTCGTTGTCGCTGTCACATTCATCACTGCTACTGCCACTGTCACTGCTGTGACCTGATATACCATCCTTCATCCTCTGGAACATGTTTCTCCTCTCCCTCCTATTTCTCCCTTGACATTTTGCCGCAGTGGTGCCGGTAACAATGGTGGGGCCAGTAGCGGTGTGATTGTAGCGTGTTTGGTTGCATGTGTTACTTTGATATTTGGAAGCATGTCCAGGTTGATAAATCACCTCGGTTTGGCTATAGCATTGGGAATTGGTTCCATCATTATGGTGTCCTTTGTAGAAGTCAGTTACCTTATGTCCGATTGAGCTATGTTGCTGCTGCTGGTGGTAGTTGTTTTTGTGCTGGCAGCTTTGTTCACAGGATTTGTAGCACTGAGCAGAGGCCATTGCAAAAAACTGTGTGTTGTTTGGTTTTGTAGTGATGTTTGTTTGCTTGTTGCTTATCGTTTTCCCCGCGT
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02827 Ps_contig_mira-and-tgicl-ass_franssen_25018
SEQUENCE=GWYRTCGGGGATCCTTCACTACCAAATATCACATTTCAATTGATCTTGAATTGAGTACAATGGATTCCAAATCATCATTCCTCATCCTTGGCCTAATGGCCATGGTTCTTCTTATTTCCTTAGAGGTGGCAGCTAGGGACTTAACTGAGACTTCCACTAATACCAAAGAGGAAGTTGTTGAAAAGTCAAATGAAATAACTGATGCCAAATATGGTGGTTATGGAGGCTACGGTCGTGGCGGTGGTTACCATAATGGTGGTGGTGGTTACAATAATGGCGGCGGTTATCATAATGGTGGAGGGGGTTACAATAACGGTGGCGGATACCATAACGGTGGTGGCGGTTACAACAACGGTGGTGGTTACCATAATGGCGGAGGTGGTTACAACAATGGTGGAGGTTACCATAATGGCGGAGGTGGTTATAACAACGGTGGAGGTTACCATAATGGTGGTGGCGGTTACAATGGTGGTGGCGGTTACCATAATGGTGGTGGTGGTTACCATGGTGGAGGTGGACATGGTGGTTACAACAATGGTAACTGATTAGATCAATGTTCTGATTAATAAAATTTCATGAATAAATAATGGTAAGTGTTGAACAAAAGAGGTATAGAGAGACTGCAATCTTTTGTGTTTATGTATTAATCTATCTTCCCACAATTCTACAAATCACTATGTATATGTAATGATGAGTTTGATTTCGGACAAAAATGTTTTGTCATTTGTAATATATCAAATATCATCCTTGTTAAGTACTTTGCATCCTTTTAAAAATTTATGTCTATAATATCTTTTTAANAATAAAAGGCATGTATTGATAC
TARGET=254,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02828 Ps_contig_mira-and-tgicl-ass_franssen_25018
SEQUENCE=GWYRTCGGGGATCCTTCACTACCAAATATCACATTTCAATTGATCTTGAATTGAGTACAATGGATTCCAAATCATCATTCCTCATCCTTGGCCTAATGGCCATGGTTCTTCTTATTTCCTTAGAGGTGGCAGCTAGGGACTTAACTGAGACTTCCACTAATACCAAAGAGGAAGTTGTTGAAAAGTCAAATGAAATAACTGATGCCAAATATGGTGGTTATGGAGGCTACGGTCGTGGCGGTGGTTACCATAATGGTGGTGGTGGTTACAATAATGGCGGCGGTTATCATAATGGTGGAGGGGGTTACAATAACGGTGGCGGATACCATAACGGTGGTGGCGGTTACAACAACGGTGGTGGTTACCATAATGGCGGAGGTGGTTACAACAATGGTGGAGGTTACCATAATGGCGGAGGTGGTTATAACAACGGTGGAGGTTACCATAATGGTGGTGGCGGTTACAATGGTGGTGGCGGTTACCATAATGGTGGTGGTGGTTACCATGGTGGAGGTGGACATGGTGGTTACAACAATGGTAACTGATTAGATCAATGTTCTGATTAATAAAATTTCATGAATAAATAATGGTAAGTGTTGAACAAAAGAGGTATAGAGAGACTGCAATCTTTTGTGTTTATGTATTAATCTATCTTCCCACAATTCTACAAATCACTATGTATATGTAATGATGAGTTTGATTTCGGACAAAAATGTTTTGTCATTTGTAATATATCAAATATCATCCTTGTTAAGTACTTTGCATCCTTTTAAAAATTTATGTCTATAATATCTTTTTAANAATAAAAGGCATGTATTGATAC
TARGET=488,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02829 Ps_contig_mira-and-tgicl-ass_franssen_25021
SEQUENCE=CTTACCTTCTATTTTGTATTTTCTATTACTTTTAAAATTTTAAGGGGTAAACTGAATATACATCAATCTCATTCAGCATTATAAAAGCACATGATAATCCTAATGACCATCATAAACCTCTAAAGAAAATTCACATATTCAAATTACACATAGTCAATAAGAAAAAGCATAAGAGAACTTCTTCTTCTTGTACATTCTTCAACCTCCAAATCCGTACAAGGTTCTTCCCTGTCTCTTGAGAGCGTAAACAACATCCATAGCTGTAACCGTCTTCCTCCTAGCGTGTTCGGTATATGTAACAGCATAATGAGGTTGAGCTTTCAGCATTAGGAATGGCTATTGCCACAGT
TARGET=178,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02830 Ps_contig_mira-and-tgicl-ass_franssen_25028
SEQUENCE=CCTTCCTCTCCTTTTTCCTTCTTTGTTTGCACAAGAAATTAGTGGTGGACAGAGGCAAACAACCTGTGAGCATTTGGCAGATACATACAGGGGAGGTATGCTTCACGGATGCTAGCTGTGATGATCACTGCAAGAACAAAGCGCACTTAATCAGTGGCACTTGCCATAACTTTAAATGTTTCTGCACTCAAAACTGTTAAACTTGGATGGATGGATTGATTCTCCAACACAAAGACAAAGACGCATAATATAAATAAATAAATAAAATGCATGCTGTAGTAGCTTGCACCGCATCTATCTATGTATATGAGTATTGCGTCTTCTTTGAATCATATATATGTGTAATAAATATCGTGCATAACGTATCAGTTGTGCTACTTTATCATATAGGAAATAAAAATATTATTAATACAA
TARGET=204,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0312
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.026872
PRIMER_LEFT_SEQUENCE=GAAATTAGTGGTGGACAGAGGC
PRIMER_RIGHT_SEQUENCE=TAGATAGATGCGGTGCAAGCTA
PRIMER_LEFT=34,22
PRIMER_RIGHT=300,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.027
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.1223
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.026872
PRIMER_LEFT_1_SEQUENCE=ATTTGGCAGATACATACAGGGG
PRIMER_RIGHT_1_SEQUENCE=TAGATAGATGCGGTGCAAGCTA
PRIMER_LEFT_1=71,22
PRIMER_RIGHT_1=300,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=60.027
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.1913
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.186972
PRIMER_LEFT_2_SEQUENCE=GAAATTAGTGGTGGACAGAGGC
PRIMER_RIGHT_2_SEQUENCE=CGTCTTTGTCTTTGTGTTGGAG
PRIMER_LEFT_2=34,22
PRIMER_RIGHT_2=242,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=59.813
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.2153
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.210973
PRIMER_LEFT_3_SEQUENCE=GAAATTAGTGGTGGACAGAGGC
PRIMER_RIGHT_3_SEQUENCE=ATGCGTCTTTGTCTTTGTGTTG
PRIMER_LEFT_3=34,22
PRIMER_RIGHT_3=245,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=60.211
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.2824
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=0.186972
PRIMER_LEFT_4_SEQUENCE=ATTTGGCAGATACATACAGGGG
PRIMER_RIGHT_4_SEQUENCE=CGTCTTTGTCTTTGTGTTGGAG
PRIMER_LEFT_4=71,22
PRIMER_RIGHT_4=242,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_02831 Ps_contig_mira-and-tgicl-ass_franssen_25028
SEQUENCE=CCTTCCTCTCCTTTTTCCTTCTTTGTTTGCACAAGAAATTAGTGGTGGACAGAGGCAAACAACCTGTGAGCATTTGGCAGATACATACAGGGGAGGTATGCTTCACGGATGCTAGCTGTGATGATCACTGCAAGAACAAAGCGCACTTAATCAGTGGCACTTGCCATAACTTTAAATGTTTCTGCACTCAAAACTGTTAAACTTGGATGGATGGATTGATTCTCCAACACAAAGACAAAGACGCATAATATAAATAAATAAATAAAATGCATGCTGTAGTAGCTTGCACCGCATCTATCTATGTATATGAGTATTGCGTCTTCTTTGAATCATATATATGTGTAATAAATATCGTGCATAACGTATCAGTTGTGCTACTTTATCATATAGGAAATAAAAATATTATTAATACAA
TARGET=250,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0312
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.026872
PRIMER_LEFT_SEQUENCE=GAAATTAGTGGTGGACAGAGGC
PRIMER_RIGHT_SEQUENCE=TAGATAGATGCGGTGCAAGCTA
PRIMER_LEFT=34,22
PRIMER_RIGHT=300,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.027
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.0511
PRIMER_LEFT_1_PENALTY=0.024206
PRIMER_RIGHT_1_PENALTY=0.026872
PRIMER_LEFT_1_SEQUENCE=ACTTGGATGGATGGATTGATTC
PRIMER_RIGHT_1_SEQUENCE=TAGATAGATGCGGTGCAAGCTA
PRIMER_LEFT_1=200,22
PRIMER_RIGHT_1=300,22
PRIMER_LEFT_1_TM=60.024
PRIMER_RIGHT_1_TM=60.027
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.1223
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.026872
PRIMER_LEFT_2_SEQUENCE=ATTTGGCAGATACATACAGGGG
PRIMER_RIGHT_2_SEQUENCE=TAGATAGATGCGGTGCAAGCTA
PRIMER_LEFT_2=71,22
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.027
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.1792
PRIMER_LEFT_3_PENALTY=0.152322
PRIMER_RIGHT_3_PENALTY=0.026872
PRIMER_LEFT_3_SEQUENCE=CTTGGATGGATGGATTGATTCT
PRIMER_RIGHT_3_SEQUENCE=TAGATAGATGCGGTGCAAGCTA
PRIMER_LEFT_3=201,22
PRIMER_RIGHT_3=300,22
PRIMER_LEFT_3_TM=60.152
PRIMER_RIGHT_3_TM=60.027
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=0.4196
PRIMER_LEFT_4_PENALTY=0.004359
PRIMER_RIGHT_4_PENALTY=0.415257
PRIMER_LEFT_4_SEQUENCE=GAAATTAGTGGTGGACAGAGGC
PRIMER_RIGHT_4_SEQUENCE=TAGATGCGGTGCAAGCTACTAC
PRIMER_LEFT_4=34,22
PRIMER_RIGHT_4=296,22
PRIMER_LEFT_4_TM=60.004
PRIMER_RIGHT_4_TM=59.585
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=5.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_02832 Ps_contig_mira-and-tgicl-ass_franssen_25037
SEQUENCE=CCCTAAATACCTTTTCCTAATAGCTTTTGTCAACTTTCCTTTGCACAAGCTTCACAATRCATATCTAACTTTKAGTACTATCTCTTGAGGAAGTTGTTCTTCATCTTTCTTTTCTTCCTTTTCTCTTCTTCCTTCTTTTCCTCTTTACTTTCTTCTTTCTTTCCTTCTTCCTTCTTCTCTTCACCCATTTCTAGAAATCTTGCTGGCAACAAAAGAGTGTGATATATATATATACTAATTGATATCMCCSCGWACT
TARGET=222,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02833 Ps_contig_mira-and-tgicl-ass_franssen_25038
SEQUENCE=GGACATCAATTAACTCCTAGATAACACACACACAACACAAAAAATGGGTAAAGATGTTGATGGAAAATTGTCTTCACCACCTTACAATCAAAGGATTCTTCTTCACAAAACCTATTTCTTTTTCATCTTCTTCTTCTTCTTCTTCTTCTTTGAAATTTAGGTGCAACAA
TARGET=24,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02834 Ps_contig_mira-and-tgicl-ass_franssen_25038
SEQUENCE=GGACATCAATTAACTCCTAGATAACACACACACAACACAAAAAATGGGTAAAGATGTTGATGGAAAATTGTCTTCACCACCTTACAATCAAAGGATTCTTCTTCACAAAACCTATTTCTTTTTCATCTTCTTCTTCTTCTTCTTCTTCTTTGAAATTTAGGTGCAACAA
TARGET=126,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02835 Ps_contig_mira-and-tgicl-ass_franssen_25051
SEQUENCE=AAGAGTAAAACGTAGAAACTTTTAGTACCAGAGTTTTGGGAGAGTGATATGGCACAGTGGTCGTTGCTTATCGTTTTGTTCTGCGTCGCCTCCGCCGCCGCCGGATTCAGCTTCCATGACTCCAATCCTATTCGAATGGTCTCCGACGTGGAGGAGCAGTTACTTCAGGTGATCGGAGAAAGTCGTCACGCCGTCTCTTCGCCGTTTCNCTAACAGATACGGTAAGCGATACGATA
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3085
PRIMER_LEFT_PENALTY=0.061818
PRIMER_RIGHT_PENALTY=0.246726
PRIMER_LEFT_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_SEQUENCE=GACTTTCTCCGATCACCTGAAG
PRIMER_LEFT=55,22
PRIMER_RIGHT=183,22
PRIMER_LEFT_TM=60.062
PRIMER_RIGHT_TM=60.247
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=129
PRIMER_PAIR_PENALTY_1=0.3637
PRIMER_LEFT_1_PENALTY=0.061818
PRIMER_RIGHT_1_PENALTY=0.301926
PRIMER_LEFT_1_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_1_SEQUENCE=CCTGAAGTAACTGCTCCTCCAC
PRIMER_LEFT_1=55,22
PRIMER_RIGHT_1=168,22
PRIMER_LEFT_1_TM=60.062
PRIMER_RIGHT_1_TM=60.302
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=0.3637
PRIMER_LEFT_2_PENALTY=0.061818
PRIMER_RIGHT_2_PENALTY=0.301926
PRIMER_LEFT_2_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_2_SEQUENCE=CACCTGAAGTAACTGCTCCTCC
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=170,22
PRIMER_LEFT_2_TM=60.062
PRIMER_RIGHT_2_TM=60.302
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=0.3717
PRIMER_LEFT_3_PENALTY=0.061818
PRIMER_RIGHT_3_PENALTY=0.309858
PRIMER_LEFT_3_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_3_SEQUENCE=GAAGTAACTGCTCCTCCACGTC
PRIMER_LEFT_3=55,22
PRIMER_RIGHT_3=165,22
PRIMER_LEFT_3_TM=60.062
PRIMER_RIGHT_3_TM=60.310
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=111
PRIMER_PAIR_PENALTY_4=0.4307
PRIMER_LEFT_4_PENALTY=0.184005
PRIMER_RIGHT_4_PENALTY=0.246726
PRIMER_LEFT_4_SEQUENCE=CGTTGCTTATCGTTTTGTTCTG
PRIMER_RIGHT_4_SEQUENCE=GACTTTCTCCGATCACCTGAAG
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=183,22
PRIMER_LEFT_4_TM=59.816
PRIMER_RIGHT_4_TM=60.247
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_02836 Ps_contig_mira-and-tgicl-ass_franssen_25057
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATCTCTGGTGTGTTGTATGGATTAGGTGTCGAGACAGACTAATTGAAAGAAAGAAAAGTNTAATTTGGGTTAGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTTACNGTGTACACTT
TARGET=157,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02837 Ps_contig_mira-and-tgicl-ass_franssen_25064
SEQUENCE=AAGCAGTGTATCAACGCAGAGTTGTTTTTTGGTTTTTAAGTTTTTGTAAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGATTTCTTTTTTACTGTGTACACTTCCTAACCG
TARGET=193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.5800
PRIMER_LEFT_PENALTY=0.371199
PRIMER_RIGHT_PENALTY=4.208770
PRIMER_LEFT_SEQUENCE=ATACGTTCTTCAATGGCGAAAC
PRIMER_RIGHT_SEQUENCE=CGGTTAGGAAGTGTACACAGTAAAA
PRIMER_LEFT=119,22
PRIMER_RIGHT=276,25
PRIMER_LEFT_TM=60.371
PRIMER_RIGHT_TM=58.791
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=4.6800
PRIMER_LEFT_1_PENALTY=0.471231
PRIMER_RIGHT_1_PENALTY=4.208770
PRIMER_LEFT_1_SEQUENCE=CATCAAGCAGGATCGGATAGTT
PRIMER_RIGHT_1_SEQUENCE=CGGTTAGGAAGTGTACACAGTAAAA
PRIMER_LEFT_1=65,22
PRIMER_RIGHT_1=276,25
PRIMER_LEFT_1_TM=60.471
PRIMER_RIGHT_1_TM=58.791
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=4.8274
PRIMER_LEFT_2_PENALTY=0.371199
PRIMER_RIGHT_2_PENALTY=4.456249
PRIMER_LEFT_2_SEQUENCE=ATACGTTCTTCAATGGCGAAAC
PRIMER_RIGHT_2_SEQUENCE=CGGTTAGGAAGTGTACACAGTAAA
PRIMER_LEFT_2=119,22
PRIMER_RIGHT_2=276,24
PRIMER_LEFT_2_TM=60.371
PRIMER_RIGHT_2_TM=57.544
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=158
PRIMER_PAIR_PENALTY_3=4.9275
PRIMER_LEFT_3_PENALTY=0.471231
PRIMER_RIGHT_3_PENALTY=4.456249
PRIMER_LEFT_3_SEQUENCE=CATCAAGCAGGATCGGATAGTT
PRIMER_RIGHT_3_SEQUENCE=CGGTTAGGAAGTGTACACAGTAAA
PRIMER_LEFT_3=65,22
PRIMER_RIGHT_3=276,24
PRIMER_LEFT_3_TM=60.471
PRIMER_RIGHT_3_TM=57.544
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=5.0917
PRIMER_LEFT_4_PENALTY=0.882980
PRIMER_RIGHT_4_PENALTY=4.208770
PRIMER_LEFT_4_SEQUENCE=ATCAAGCAGGATCGGATAGTTT
PRIMER_RIGHT_4_SEQUENCE=CGGTTAGGAAGTGTACACAGTAAAA
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=276,25
PRIMER_LEFT_4_TM=59.117
PRIMER_RIGHT_4_TM=58.791
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_02838 Ps_contig_mira-and-tgicl-ass_franssen_25074
SEQUENCE=CGGGGTTACTAACTAACTATTTTGTCTTAGTTAGTCATTCGAAGAGAACAACATCAAAGTGCAAGGAGTCTCAACGCCCTCATGGCCACCTCTGCTATCCAACAATCAGCTTTCACTGGTAAGACTGGTTTGAGGCAGGGAAATGAATTCATCAGGAAAGCTGGAAACTTTGGCCAAGCTCGCTTTACCATGCGTCGTACTGTGAAGAGTGCTCCTAGAAAGCTATTTGGTA
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02839 Ps_contig_mira-and-tgicl-ass_franssen_25122
SEQUENCE=ATAAAGGGTAGAACAACAAACACGCGNRGAGAACAACAACAACAAGAAGAACAAGGGTTTTTCACTTCCTCGTTTAAATCGAAAACATTAACCAAAACATGGGCAGAAAGTTCTTCGTCGGCGGTAACTGGAAATGCAATGGAACCACTGAAGAGGTGAAGAAAATTGTGGGTACTTTGAACGAAGCTAAAGTCCCCGGAGAAGATGTTGTTGAAGTTGTTGTGAGCCCCCCTTAGTCTTCCTTCCTTTCGTGAAAAGCTTGCTTCGCTCTGACTTCCATGTTGCTGCTACAAAACGGTTGGGTTCGC
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02840 Ps_contig_mira-and-tgicl-ass_franssen_25135
SEQUENCE=CTTACCCCTCCGTTTGCGTGTCGTCTACGAAATAATTTTACTAATACTGCTGAAGAGAGATCCATGACCACATATATATATTCTAAAAAGACAAACACACATGTGTACTCATACACACAATTAAACATAATGGTTTCAATATCTCTGCCTCAAGCTTTTATATGTTACACTTCATTTGCAGTTGCAAGGGTCACAAGTGCAGTTATCACCACACTTGCAGCCACCATCCTCAGAAGCAGCACTCATTTCAGCACCTTCAAACTGGATCTTCGCCGGACCGACGCCAAGAATCACGGTTTCGGTGGTTTCCATTTCGGAGTAGCTCAATCCACTAGACCTCTTGTTGCATTTGCAGCTATCACCACAGTTGCAACTGCTTCCACAACCACATCCAGACATTATTTTCAAGAAACTAAGTATTTTTGACAAACAACAATTGATATAANTTAATTGGTTGNTNGTTTNRTCGTTNNYTAYTANCTTTCCTAACCTTAACCTAAATTTACG
TARGET=72,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02841 Ps_contig_mira-and-tgicl-ass_franssen_25147
SEQUENCE=CTTCTTCTTCTTTTCGTCTCAAGAACAAATCGCGATTCGCTCTTCTCCGAAAAACCTCTAGATCGAAAAGAAGAGCGGTTTTGTGTGTTGGTGAAGATGGGGTTAACGTTCACGAAGCTGTTCAGTAGGTTATTCGCGAAGAAGGAAATGAAGGATTTTGATGGTTGGTCTCGATGCTGCTGATAAGACAACTATTTTGCATAAACTCAAGCTATGGCATCATCAAGTGG
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02842 Ps_contig_mira-and-tgicl-ass_franssen_25183
SEQUENCE=CGTCGGGGATCCTTCACTACCAAATATCACATTTCAATCCATCTTGAATTGAGTACAATGGATTCCAAAAATGCAATCCTCATCCTTGGCCTATTGGCCATGCTTCTTGTTATTTCCTCACAAGTGTCAGCTAGGGACTTAACTGAGGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGACACTACGGTCATGGTGGTGGAAGTTACCATGGTGGTGGCGGCAGTTACCACGGTGGACATGGTGGACATGGAGGACATGGTGGACATGGTGGTGTTTCCAACAATGAGGTTGTTGAAACTTCAAATGAAGTAAAAGATGCCAAATATGGACACTACGGTCATGGTGGTGGTAGTTACCATGGTGGTGGTGGCGGTTACCACGGTGGACATGGAGGACATGGTGGACATGGTGGACACGGTGGTGCTTCAGACAATGGAAACTGATTATATCATCATACATGATGCACATTCAAAGTAATAAGTTTCATGAATGAATAGTGTTTGGTGTAAAATAAAAGAGGTATCATCTTTGTATTTGTGGTGAAGAGTTTGATTCGAGTGAAAGTGATGAATGTGTTATCATTTGTAATATATCAAATATATATTATTGTTGTTTAGTACTTTGATTAAAA
TARGET=372,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4000
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=0.146525
PRIMER_LEFT_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_SEQUENCE=CGAATCAAACTCTTCACCACAA
PRIMER_LEFT=331,22
PRIMER_RIGHT=568,22
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=60.147
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.4734
PRIMER_LEFT_1_PENALTY=0.253482
PRIMER_RIGHT_1_PENALTY=0.219884
PRIMER_LEFT_1_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_1_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT_1=331,22
PRIMER_RIGHT_1=454,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=59.780
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.4734
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.219884
PRIMER_LEFT_2_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_2_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT_2=181,22
PRIMER_RIGHT_2=454,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=59.780
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=0.7811
PRIMER_LEFT_3_PENALTY=0.634599
PRIMER_RIGHT_3_PENALTY=0.146525
PRIMER_LEFT_3_SEQUENCE=GATGCCAAATATGGACACTACG
PRIMER_RIGHT_3_SEQUENCE=CGAATCAAACTCTTCACCACAA
PRIMER_LEFT_3=327,22
PRIMER_RIGHT_3=568,22
PRIMER_LEFT_3_TM=59.365
PRIMER_RIGHT_3_TM=60.147
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.7905
PRIMER_LEFT_4_PENALTY=0.253482
PRIMER_RIGHT_4_PENALTY=0.536975
PRIMER_LEFT_4_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_4_SEQUENCE=AGTTTCCATTGTCTGAAGCACC
PRIMER_LEFT_4=331,22
PRIMER_RIGHT_4=453,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=60.537
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_02843 Ps_contig_mira-and-tgicl-ass_franssen_25185
SEQUENCE=TACACTGCTTAAGCATGGNARTCNCRNARACNTANTCGGRGATCCAAACAACAACAACACTATCATCATCGTCTCATACTTTACCTTTCATTTCAGAGTTACAGCTACATAATCCATTTTAGTTACAGTTAATTAATTTCCAGTTCGATCCATCTTGTTAGTAGTGATCATCATGGCTGAGGAGAATCAGAACAAGTACGAGGAGACTACCTCCGCAACCAACTCTGAGACAGAGATCAAGGACAGGTAGGTTTGTTTTTTGTTACGTCNCGGGTAGGTAAAAAGGAAAAGGNTAGAAAGAACATAAGCCTGCTACAAGATGGA
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02844 Ps_contig_mira-and-tgicl-ass_franssen_25202
SEQUENCE=CCGGGGAACTCTCCAGTCAAGTAGGATGGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCACGGGCTTCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGCTTGACGGGGTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATGTGTGTGTATTA
TARGET=214,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02845 Ps_contig_mira-and-tgicl-ass_franssen_25230
SEQUENCE=AGAAATGCAGGGGTTACTTTTGACCCTTTGTTGGTAGGGTGAAAAATCGTGATCGCATTCTTCTTCTTCCTGTGGTTGTTTGTTCCTTCCTTCTCTCTCTCTCACTGTCTCACTTCCTCAACCAAAACCCTAATCCTTTCACATCGCAATCAAATCATGGACGCTGATTCTTCCTGGACTGCTCGTCTCTCTTCCGCATCCAGACGTTATCAAGCCGCTCTTCAATCTCGATCAGATATGTTCATGGGTTTTGATGAAAACGACGTGGAAGAAGATATAAGGGAAGAGTTTCTGTGTCCGTTTTGTTCCGAGTATTTTGATATTGTTGGGTTGTGCTGCCATATTGATGAAGAGCATCCAATGGAGGCAAAAAATGGGGTATGTCCAGTTTGTGCATTGAGGGTGGGGGTTGATATGGTTGCTCACATAACCTTACAACATGGAAATATATTTAAGATGCAGCGCAAGAGGAAATCACGGAAAGGTGGATCTTATTCAACACTTTCTTTATTGAGGAAGGAGCTGAGAGAAGGAAATTTGCAATCCCTTTTTGGTGGGTCTCCATGTATAGTGTCCTCGTCTAATGCAGCACCTGATCCGCTGCTGTCGTCATTCATATCGCCTTTAAGTAATGAATCTATGAGTTCTCAGTCTCACACACAAACTGAGATTAGATCATCCAAGAAATGCTCAGATGAGAATGTAACCAAAAAGCCATGTGGAGACACCAACCATGTCAAGTTAAAGATAAGGAGGAGAAGACGAGAAGATGTGAGTTTATTCAAGGGATGTTGATATCCACCATACTTGATGACGATTTATGAAAAGAAACTCATCA
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3088
PRIMER_LEFT_PENALTY=0.099187
PRIMER_RIGHT_PENALTY=0.209637
PRIMER_LEFT_SEQUENCE=CTTTTGACCCTTTGTTGGTAGG
PRIMER_RIGHT_SEQUENCE=TGTGAAAGGATTAGGGTTTTGG
PRIMER_LEFT=16,22
PRIMER_RIGHT=142,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=60.210
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=127
PRIMER_PAIR_PENALTY_1=0.3305
PRIMER_LEFT_1_PENALTY=0.099187
PRIMER_RIGHT_1_PENALTY=0.231280
PRIMER_LEFT_1_SEQUENCE=CTTTTGACCCTTTGTTGGTAGG
PRIMER_RIGHT_1_SEQUENCE=GATTGAAGAGCGGCTTGATAAC
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=226,22
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=60.231
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.3480
PRIMER_LEFT_2_PENALTY=0.099187
PRIMER_RIGHT_2_PENALTY=0.248781
PRIMER_LEFT_2_SEQUENCE=CTTTTGACCCTTTGTTGGTAGG
PRIMER_RIGHT_2_SEQUENCE=TTTTGGTTGAGGAAGTGAGACA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=126,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=59.751
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=0.3815
PRIMER_LEFT_3_PENALTY=0.099187
PRIMER_RIGHT_3_PENALTY=0.282356
PRIMER_LEFT_3_SEQUENCE=CTTTTGACCCTTTGTTGGTAGG
PRIMER_RIGHT_3_SEQUENCE=ATACTCGGAACAAAACGGACAC
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=60.282
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.4311
PRIMER_LEFT_4_PENALTY=0.099187
PRIMER_RIGHT_4_PENALTY=0.331942
PRIMER_LEFT_4_SEQUENCE=CTTTTGACCCTTTGTTGGTAGG
PRIMER_RIGHT_4_SEQUENCE=ATTGCGATGTGAAAGGATTAGG
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=149,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=60.332
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_02846 Ps_contig_mira-and-tgicl-ass_franssen_25230
SEQUENCE=AGAAATGCAGGGGTTACTTTTGACCCTTTGTTGGTAGGGTGAAAAATCGTGATCGCATTCTTCTTCTTCCTGTGGTTGTTTGTTCCTTCCTTCTCTCTCTCTCACTGTCTCACTTCCTCAACCAAAACCCTAATCCTTTCACATCGCAATCAAATCATGGACGCTGATTCTTCCTGGACTGCTCGTCTCTCTTCCGCATCCAGACGTTATCAAGCCGCTCTTCAATCTCGATCAGATATGTTCATGGGTTTTGATGAAAACGACGTGGAAGAAGATATAAGGGAAGAGTTTCTGTGTCCGTTTTGTTCCGAGTATTTTGATATTGTTGGGTTGTGCTGCCATATTGATGAAGAGCATCCAATGGAGGCAAAAAATGGGGTATGTCCAGTTTGTGCATTGAGGGTGGGGGTTGATATGGTTGCTCACATAACCTTACAACATGGAAATATATTTAAGATGCAGCGCAAGAGGAAATCACGGAAAGGTGGATCTTATTCAACACTTTCTTTATTGAGGAAGGAGCTGAGAGAAGGAAATTTGCAATCCCTTTTTGGTGGGTCTCCATGTATAGTGTCCTCGTCTAATGCAGCACCTGATCCGCTGCTGTCGTCATTCATATCGCCTTTAAGTAATGAATCTATGAGTTCTCAGTCTCACACACAAACTGAGATTAGATCATCCAAGAAATGCTCAGATGAGAATGTAACCAAAAAGCCATGTGGAGACACCAACCATGTCAAGTTAAAGATAAGGAGGAGAAGACGAGAAGATGTGAGTTTATTCAAGGGATGTTGATATCCACCATACTTGATGACGATTTATGAAAAGAAACTCATCA
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3088
PRIMER_LEFT_PENALTY=0.099187
PRIMER_RIGHT_PENALTY=0.209637
PRIMER_LEFT_SEQUENCE=CTTTTGACCCTTTGTTGGTAGG
PRIMER_RIGHT_SEQUENCE=TGTGAAAGGATTAGGGTTTTGG
PRIMER_LEFT=16,22
PRIMER_RIGHT=142,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=60.210
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=127
PRIMER_PAIR_PENALTY_1=0.3305
PRIMER_LEFT_1_PENALTY=0.099187
PRIMER_RIGHT_1_PENALTY=0.231280
PRIMER_LEFT_1_SEQUENCE=CTTTTGACCCTTTGTTGGTAGG
PRIMER_RIGHT_1_SEQUENCE=GATTGAAGAGCGGCTTGATAAC
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=226,22
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=60.231
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.3480
PRIMER_LEFT_2_PENALTY=0.099187
PRIMER_RIGHT_2_PENALTY=0.248781
PRIMER_LEFT_2_SEQUENCE=CTTTTGACCCTTTGTTGGTAGG
PRIMER_RIGHT_2_SEQUENCE=TTTTGGTTGAGGAAGTGAGACA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=126,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=59.751
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=0.3815
PRIMER_LEFT_3_PENALTY=0.099187
PRIMER_RIGHT_3_PENALTY=0.282356
PRIMER_LEFT_3_SEQUENCE=CTTTTGACCCTTTGTTGGTAGG
PRIMER_RIGHT_3_SEQUENCE=ATACTCGGAACAAAACGGACAC
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=60.282
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.4311
PRIMER_LEFT_4_PENALTY=0.099187
PRIMER_RIGHT_4_PENALTY=0.331942
PRIMER_LEFT_4_SEQUENCE=CTTTTGACCCTTTGTTGGTAGG
PRIMER_RIGHT_4_SEQUENCE=ATTGCGATGTGAAAGGATTAGG
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=149,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=60.332
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_02847 Ps_contig_mira-and-tgicl-ass_franssen_25236
SEQUENCE=TGTAAGGTGAAAGAACTGAAGAATGGTAGGTTAGCGATGTTCTCAATGTTTGGATTCTTCGTTCAAGCTATTGTAACAGGAAAAGGACCAATAGAAAATCTTGCTGACCACCTGGCTGATCCAGTGAACAACAATGCATGGGCATATGCCACAAAACTTTCGTCCCCCGTACGGGAAAAGGTTGGAAGGAAGGAAAAAATTAACGGAACCTAAATAGGAATTAAAGGTACGGTAACTTAAACCGTTTAGTAATTTAATTCCGACCCCAACAAAACGTTGGTTTAGGTTAAG
TARGET=184,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0557
PRIMER_LEFT_PENALTY=0.130883
PRIMER_RIGHT_PENALTY=1.924791
PRIMER_LEFT_SEQUENCE=TAGCGATGTTCTCAATGTTTGG
PRIMER_RIGHT_SEQUENCE=GTTTTGTTGGGGTCGGAATTA
PRIMER_LEFT=31,22
PRIMER_RIGHT=274,21
PRIMER_LEFT_TM=60.131
PRIMER_RIGHT_TM=60.925
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=2.1561
PRIMER_LEFT_1_PENALTY=0.231323
PRIMER_RIGHT_1_PENALTY=1.924791
PRIMER_LEFT_1_SEQUENCE=TGGTAGGTTAGCGATGTTCTCA
PRIMER_RIGHT_1_SEQUENCE=GTTTTGTTGGGGTCGGAATTA
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=274,21
PRIMER_LEFT_1_TM=59.769
PRIMER_RIGHT_1_TM=60.925
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=2.1688
PRIMER_LEFT_2_PENALTY=0.243965
PRIMER_RIGHT_2_PENALTY=1.924791
PRIMER_LEFT_2_SEQUENCE=ATATGCCACAAAACTTTCGTCC
PRIMER_RIGHT_2_SEQUENCE=GTTTTGTTGGGGTCGGAATTA
PRIMER_LEFT_2=143,22
PRIMER_RIGHT_2=274,21
PRIMER_LEFT_2_TM=60.244
PRIMER_RIGHT_2_TM=60.925
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=2.2905
PRIMER_LEFT_3_PENALTY=0.130883
PRIMER_RIGHT_3_PENALTY=2.159665
PRIMER_LEFT_3_SEQUENCE=TAGCGATGTTCTCAATGTTTGG
PRIMER_RIGHT_3_SEQUENCE=TTTTGTTGGGGTCGGAATTA
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=273,20
PRIMER_LEFT_3_TM=60.131
PRIMER_RIGHT_3_TM=60.160
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=2.2905
PRIMER_LEFT_4_PENALTY=0.130883
PRIMER_RIGHT_4_PENALTY=2.159665
PRIMER_LEFT_4_SEQUENCE=TAGCGATGTTCTCAATGTTTGG
PRIMER_RIGHT_4_SEQUENCE=TTGTTGGGGTCGGAATTAAA
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=271,20
PRIMER_LEFT_4_TM=60.131
PRIMER_RIGHT_4_TM=60.160
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_02848 Ps_contig_mira-and-tgicl-ass_franssen_25238
SEQUENCE=GTCACCGGGGAACTCTCCAGTCAAGTAGGACGGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCAGAGGAGGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCACCTCCCAATTCTTGGCTTGATGGGTTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCACGGATGATGATGATGATGCCGGCCATCGTGTTATTATTATTGCAA
TARGET=218,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02849 Ps_contig_mira-and-tgicl-ass_franssen_25242
SEQUENCE=CTCACCAGTACCCTTACATTCATTGCAGGGATGCTGCATTTGCTGAATCATTGATGGACCGAGATGCCTAATAGAAACCTTCATACCAGTTCCTTGACAACCAGCACATTTCATGGAAGCTCCAGATTTAGAACCTTTGCCACTGCACTTTGAGCACAAGACATTCCTCGAGAGAGAAAGCTTCTTAGATGTTCCGAGATAAAGATCCTCCAGAGAGACCTTGAGAGGGTGAACAACATCTTCTCCACGTCTCTGCCTCCGTCCACGGCTACTACCACCGGCACCACCACCAAAGAAAGACGAGAAGATGTCAAATGGGTCATGGCCACCGCCACCACCACCACCCATTCCTTCCTTAAGAGCATCCTCACCATATGTATCATATATCTCACGCTTCTCAGGATCACTCAGAACATCATAAGCTTGTGCTAGCTCTTTGAACTTCTCGGGATCACCACCTTTATCGGGATGATTCTTAATAGCAGCTTTCTTGTAAGCTTTCTTCAAATCATCCTGAGACGCGTTCTTAGAAACACCAAGGATTTCATAGTACCTCGTACTATCACTTTTCTTCGGTGCTCTCCCAAACATCGTTGTACTCGAAAATCTAGTACAGATCGAAACCTAAAACCTAAATTGGAAGAAGCGATCAACGAGAGGAAGAAAAGGAGTTCTAGATCGGAATAAACTAGAAAGAGCAGCAACTAATTGGGCACGCTAATTTGTTATATTGGATTTGGTTGGGTTGGGAGTCAGATTGAATGGACGGTTCCCCGCGTACTCTGCGTTG
TARGET=332,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0231
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.022341
PRIMER_LEFT_SEQUENCE=CCAGATTTAGAACCTTTGCCAC
PRIMER_RIGHT_SEQUENCE=TGATCCTGAGAAGCGTGAGATA
PRIMER_LEFT=121,22
PRIMER_RIGHT=405,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.978
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.0954
PRIMER_LEFT_1_PENALTY=0.073038
PRIMER_RIGHT_1_PENALTY=0.022341
PRIMER_LEFT_1_SEQUENCE=GCACTTTGAGCACAAGACATTC
PRIMER_RIGHT_1_SEQUENCE=TGATCCTGAGAAGCGTGAGATA
PRIMER_LEFT_1=144,22
PRIMER_RIGHT_1=405,22
PRIMER_LEFT_1_TM=59.927
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.1218
PRIMER_LEFT_2_PENALTY=0.099438
PRIMER_RIGHT_2_PENALTY=0.022341
PRIMER_LEFT_2_SEQUENCE=ACCTTGAGAGGGTGAACAACAT
PRIMER_RIGHT_2_SEQUENCE=TGATCCTGAGAAGCGTGAGATA
PRIMER_LEFT_2=217,22
PRIMER_RIGHT_2=405,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=59.978
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.1479
PRIMER_LEFT_3_PENALTY=0.125540
PRIMER_RIGHT_3_PENALTY=0.022341
PRIMER_LEFT_3_SEQUENCE=TCCTCCAGAGAGACCTTGAGAG
PRIMER_RIGHT_3_SEQUENCE=TGATCCTGAGAAGCGTGAGATA
PRIMER_LEFT_3=205,22
PRIMER_RIGHT_3=405,22
PRIMER_LEFT_3_TM=60.126
PRIMER_RIGHT_3_TM=59.978
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=0.1583
PRIMER_LEFT_4_PENALTY=0.135999
PRIMER_RIGHT_4_PENALTY=0.022341
PRIMER_LEFT_4_SEQUENCE=GATTTAGAACCTTTGCCACTGC
PRIMER_RIGHT_4_SEQUENCE=TGATCCTGAGAAGCGTGAGATA
PRIMER_LEFT_4=124,22
PRIMER_RIGHT_4=405,22
PRIMER_LEFT_4_TM=60.136
PRIMER_RIGHT_4_TM=59.978
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_02850 Ps_contig_mira-and-tgicl-ass_franssen_25247
SEQUENCE=GGTTAAGTTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAATTGAGGGATACGTTCTTCAATGGCAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATGAAAGAAAGAAATATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGNGTTYYTTTTTTACTTGTGACACTT
TARGET=154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02851 Ps_contig_mira-and-tgicl-ass_franssen_25275
SEQUENCE=AAGCAGTGGTATCAACGCAGAGTGTTAAAGGCAAGGTGGTGATTGATGCTTTCCGGCTGATTAATCCACAAACTATGATGCTGGGTCAAGAACCACGACAGACAACCTCTAACCTTGGCCATCTGAACAAACCATCTATCCAAGCTCTGATCCATGGCTTGAACCGACATTATTACTCAATAGCAATCAATTACAGGAAGAACGAGCTTGAAGAGAAGATGCTGCTTAACCTTCATAAGAAGAAATGGACCGATGGTTTGACACTCAGACGATTTGATTCACATTCTAAAACTAATGAACAGACTGTTCAGGAGATGCTAAGTTTAGCAACCAAGTACAACAAGGCAGTGCAAGAGGAAGATGAGCTGCCCCCAGAAAAGCTTGCAATTGCTAATGTGGGAAGGCAGGATGCTAAGAAGCATCTTGAAGAGCACGTTTCTAATTTAATGTCTTCGAACATTGTTCAGACTTTGGGAATGATGCTCGACACAGTTGTGTTCTAGAGTTAGGATCGTCCACTGTATTCTTCTTCTTCTTCATGATGTCCAGCAATTATTTCATTTACACTTCTTTGACCTTGTTTGCAATAGTTATTTTTTTTTNTAATTAAATAAAANTTAAAATTTAAAAAAAAGT
TARGET=524,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3378
PRIMER_LEFT_PENALTY=0.019966
PRIMER_RIGHT_PENALTY=0.317850
PRIMER_LEFT_SEQUENCE=GTACAACAAGGCAGTGCAAGAG
PRIMER_RIGHT_SEQUENCE=TGCAAACAAGGTCAAAGAAGTG
PRIMER_LEFT=334,22
PRIMER_RIGHT=585,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.318
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.3378
PRIMER_LEFT_1_PENALTY=0.019966
PRIMER_RIGHT_1_PENALTY=0.317850
PRIMER_LEFT_1_SEQUENCE=AGTACAACAAGGCAGTGCAAGA
PRIMER_RIGHT_1_SEQUENCE=TGCAAACAAGGTCAAAGAAGTG
PRIMER_LEFT_1=333,22
PRIMER_RIGHT_1=585,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=60.318
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.3471
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.317850
PRIMER_LEFT_2_SEQUENCE=TGTTCAGACTTTGGGAATGATG
PRIMER_RIGHT_2_SEQUENCE=TGCAAACAAGGTCAAAGAAGTG
PRIMER_LEFT_2=460,22
PRIMER_RIGHT_2=585,22
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=60.318
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.7503
PRIMER_LEFT_3_PENALTY=0.432475
PRIMER_RIGHT_3_PENALTY=0.317850
PRIMER_LEFT_3_SEQUENCE=AAGCATCTTGAAGAGCACGTTT
PRIMER_RIGHT_3_SEQUENCE=TGCAAACAAGGTCAAAGAAGTG
PRIMER_LEFT_3=416,22
PRIMER_RIGHT_3=585,22
PRIMER_LEFT_3_TM=60.432
PRIMER_RIGHT_3_TM=60.318
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=0.8296
PRIMER_LEFT_4_PENALTY=0.511768
PRIMER_RIGHT_4_PENALTY=0.317850
PRIMER_LEFT_4_SEQUENCE=AAGTACAACAAGGCAGTGCAAG
PRIMER_RIGHT_4_SEQUENCE=TGCAAACAAGGTCAAAGAAGTG
PRIMER_LEFT_4=332,22
PRIMER_RIGHT_4=585,22
PRIMER_LEFT_4_TM=59.488
PRIMER_RIGHT_4_TM=60.318
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_02852 Ps_contig_mira-and-tgicl-ass_franssen_25283
SEQUENCE=CTTAGGAGAATGTCTCTGGGTAAACAAAAGAAACAAAAGAAAGAAAGTACCGGCAGTGTCCCAACCGTAGTCACCGGGGAACTCTCCAGTCAAGTAGGATGGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCAGAGGAGGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCACCTCCCAATTCTTGGCTTGNTGGGTTCAGCTTGACTGGCTTGCCAGTCAAGGGTTGGGGAAGAGAGACG
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4994
PRIMER_LEFT_PENALTY=1.487218
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=GGAGAATGTCTCTGGGTAAACAA
PRIMER_RIGHT_SEQUENCE=CAAGAAAGTAGCCTCCTCTGGA
PRIMER_LEFT=4,23
PRIMER_RIGHT=180,22
PRIMER_LEFT_TM=59.513
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=1.6119
PRIMER_LEFT_1_PENALTY=1.487218
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=GGAGAATGTCTCTGGGTAAACAA
PRIMER_RIGHT_1_SEQUENCE=TTCACCATGAGGAAGTCTGCTA
PRIMER_LEFT_1=4,23
PRIMER_RIGHT_1=206,22
PRIMER_LEFT_1_TM=59.513
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=1.7891
PRIMER_LEFT_2_PENALTY=1.487218
PRIMER_RIGHT_2_PENALTY=0.301926
PRIMER_LEFT_2_SEQUENCE=GGAGAATGTCTCTGGGTAAACAA
PRIMER_RIGHT_2_SEQUENCE=GAGGAAGTCTGCTACCACCAAG
PRIMER_LEFT_2=4,23
PRIMER_RIGHT_2=198,22
PRIMER_LEFT_2_TM=59.513
PRIMER_RIGHT_2_TM=60.302
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=1.7891
PRIMER_LEFT_3_PENALTY=1.487218
PRIMER_RIGHT_3_PENALTY=0.301926
PRIMER_LEFT_3_SEQUENCE=GGAGAATGTCTCTGGGTAAACAA
PRIMER_RIGHT_3_SEQUENCE=AGGAAGTCTGCTACCACCAAGA
PRIMER_LEFT_3=4,23
PRIMER_RIGHT_3=197,22
PRIMER_LEFT_3_TM=59.513
PRIMER_RIGHT_3_TM=60.302
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=1.8343
PRIMER_LEFT_4_PENALTY=1.487218
PRIMER_RIGHT_4_PENALTY=0.347129
PRIMER_LEFT_4_SEQUENCE=GGAGAATGTCTCTGGGTAAACAA
PRIMER_RIGHT_4_SEQUENCE=TACCACCAAGAAAGTAGCCTCC
PRIMER_LEFT_4=4,23
PRIMER_RIGHT_4=186,22
PRIMER_LEFT_4_TM=59.513
PRIMER_RIGHT_4_TM=59.653
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_02853 Ps_contig_mira-and-tgicl-ass_franssen_25290
SEQUENCE=TTCGGGGTTATTNNTYAAACTACATACATATAATATTAACATATTAGTAAGTGTTATTGAAGTGAGTTATATTTAAGTTTATATATTCATACCACTACTTAAGCCATGGAGAAGAAAGCAGTAGCTGCCTTGTCCTTCCTCTTCCTCGTTCTCTTTGTTGCACAAGAAATTGTGGTGACAGAGGCAAAAACTTGTGAGCATTTGGCTGATACATACAGGGGAGTATGCTTCACGAATGCTAGCTGTGATGATCACTGCAAGAACAAAGCGCACTTAATCAGTGGCACGTGCCATAACTGGAAATGTTTCTGCACTCAAAACTGTTAAACTTGGAGGGATGGATTGATTCTCCAACACAAAGAGATAGACGCATAATATAAATAAATAAATAAAATGCATGCTGTAGTAGCTAGCACTGCATCTATCTATGTATATGAGTTTGCGTCTTCTTTGAATCATAAATATGTGTGTAATAAATATCGTGCATAACGTATTAGTTATGCACTTTATCATATGGAATAAAAATATTATTAATAACGATCNNGTNAACNAAAAAAAGACT
TARGET=377,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02854 Ps_contig_mira-and-tgicl-ass_franssen_25293
SEQUENCE=AGTACGTCGGGGATATCATAGCATCTCTTTTCTTTCTTCTATTATTATATTTTTAGTTTCTTCTCAAAATGGTGCTAGCTGGCAAGCTTATTACTGAACTTGGAATCAAAACACCCGCTGACAAGTTTTACAAACTATTTACATTAGAATTTTCCGAAGTGCAAAACATTTGTGAAAGAGTTCATAAAGGCAAGCTGCATGAAGGTGAAGACTGGCATGACACTGATACAGTTAAACACTGGACTTATGTCATAGGTGGTGAGGTACACACATGTTATGAGAGTTTTGAAGAAGTTGATGAACAAAATAAAAAACTGACTCTCAAGCTTTTTGGTGGAGACATTGAGAAGCACTATAAGCTCTTTAAAGTCACCCTTGAAGTAGTTGATAAGGCTGATGGCACTGCTGTTGCTAAATGGACTGTTGAATATGAGAAAATCAGTGAGGATATTCATCCTCCAAATGGATGGATGGACTATCTTAGCCAATGCACTAGAGATATTGATGGTCATCTTATCAAAAATTAATCATTAAGGCATTATATGATGAATAAAGAATGTTTAATTAAAATTGGCGTGATGTTTGTAATGGTATGCATGTAACCTATACAAGCATGCATGTTTGCTTTTCTTGATGCTTCCTTTGTATGGAAAAATGTGTCGGTCATGTCATGATGATCGGGCCTTATCATGTTATTATTGTTGTAATGCAAATTAAATATTTCTGAATCATATAAATTTGGTTTGCCACCYAACC
TARGET=463,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02855 Ps_contig_mira-and-tgicl-ass_franssen_25298
SEQUENCE=TTATGAGTGTACCGTGACAAAGACTTAACCTTTGGTTTTGTATCCTACTAAGGATTTTCACTCTTACTCCANNYTAGTGGTATCAACGCCAAGTTGTATTTTTGGTGGATGAAACACCAATAAGAGTACACACAAACATGGAACATAGAGGAATTCCATATCCAAAAGACCAAGCAATGGGAGTATACAGTTCAATATGGAACGCAGATGATTGGGCAACACAAGGAGGAAGAGTGAAAACAGATTGGAGTCATGCACCTTTCCTTGTAACCTATAAATCCTTTGAAATCAATGCTTGTGAGTGTCCGGTATCAGTGGCTGGAATGGATAATAGAAAGAGATGTAGTAGTAGTGAGGATAAGAAGTATTGGTGGGATGAACCAAAACTTTCAGTCCTTAATTTGCATCAGAGTCATCAACTAATGTGGGTTAGGGCTAAACATATGGTTTATGATTATTGTAATGATGCTTCTAGGTTTCCTGTTACTCCTCTTGAGTGTCTTCATCATCGTCATAGATTATTCTAATCATGTCAAACTTGTGTATTATTATTATGGCTGGAAATGGTGAATGTTGGTGTAAGAAATGTTCCACTTGTGTCATATTATTGTATGATATTATTGGCAAACTATATATAATGTGGAGAGAAAGTGTTAAAATAAGGTGATGTCAAACTTGTGTATTATTATGGGTGGAAATGGTGAATATTGGTGTAAGAAAGTTGGGGAATATGTTCCACTTATGTCATATTATTGTATGATATTATTGGCAAACTATATATAATGTGAATTACTTGGTATATTATCTTTTGAANT
TARGET=544,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02856 Ps_contig_mira-and-tgicl-ass_franssen_25300
SEQUENCE=TGAACAGACTGTTCAGGAGATGCTAAGTTTAGCAACCAAGTACAACAAGGCAGTGCAAGAGGAAGATGAGCTGCCCCCAGAAAAGCTTGCAATTGCTAATGTGGGAAGGCAGGATGCTAAGAAGCATCTTGAAGAGCACGTTTCTAATTTAATGTCTTCGAACATTGTTCAGACTTTGGGAATGATGCTCGACACAGTTGTGTTCTAGAGTTAGGATCGTCCACTGTATTCTTCTTCTTCTTCATGATGTCCAGCAATATTTCATTTACACTCTTTGACCTGTTTGCAATAGATTGTTTTTTATTACAAAGTTATTTGCATTTCATTGTGTTCGTCTCAAATGTTTCCGGATGATAATCATTTTCTTAAGCTTTAGAGACACTGGGGACTCTGCTCAAAAATCATAGAATGCATTTGATTTTTTGTTTTTAAGTTTAAANCTAA
TARGET=229,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0445
PRIMER_LEFT_PENALTY=0.029253
PRIMER_RIGHT_PENALTY=0.015253
PRIMER_LEFT_SEQUENCE=TGTTCAGACTTTGGGAATGATG
PRIMER_RIGHT_SEQUENCE=GGAAACATTTGAGACGAACACA
PRIMER_LEFT=165,22
PRIMER_RIGHT=347,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.2007
PRIMER_LEFT_1_PENALTY=0.019966
PRIMER_RIGHT_1_PENALTY=0.180765
PRIMER_LEFT_1_SEQUENCE=GTACAACAAGGCAGTGCAAGAG
PRIMER_RIGHT_1_SEQUENCE=TTGCAAACAGGTCAAAGAGTGT
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=288,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=59.819
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.2007
PRIMER_LEFT_2_PENALTY=0.019966
PRIMER_RIGHT_2_PENALTY=0.180765
PRIMER_LEFT_2_SEQUENCE=AGTACAACAAGGCAGTGCAAGA
PRIMER_RIGHT_2_SEQUENCE=TTGCAAACAGGTCAAAGAGTGT
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=59.819
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.2100
PRIMER_LEFT_3_PENALTY=0.029253
PRIMER_RIGHT_3_PENALTY=0.180765
PRIMER_LEFT_3_SEQUENCE=TGTTCAGACTTTGGGAATGATG
PRIMER_RIGHT_3_SEQUENCE=TTGCAAACAGGTCAAAGAGTGT
PRIMER_LEFT_3=165,22
PRIMER_RIGHT_3=288,22
PRIMER_LEFT_3_TM=59.971
PRIMER_RIGHT_3_TM=59.819
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=0.4477
PRIMER_LEFT_4_PENALTY=0.432475
PRIMER_RIGHT_4_PENALTY=0.015253
PRIMER_LEFT_4_SEQUENCE=AAGCATCTTGAAGAGCACGTTT
PRIMER_RIGHT_4_SEQUENCE=GGAAACATTTGAGACGAACACA
PRIMER_LEFT_4=121,22
PRIMER_RIGHT_4=347,22
PRIMER_LEFT_4_TM=60.432
PRIMER_RIGHT_4_TM=60.015
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_02857 Ps_contig_mira-and-tgicl-ass_franssen_25338
SEQUENCE=GTCGGGGAGGAAGCAAAGGAAGAGAACTTAGAAGCACCTAAAGAAACTGAGGAATCGGTTGAAGAAGTGAAGGAAGAGGCGGAAGCAGTAGTGGAGGAGCCAATAGCAAYNACTNRAAATGTTTCTGCACTCAAAACTGTTAAACTTGGAGGGATGGATTGATTCTCCAACACAAAGAGATAGACGCATAATATAAATAAATAAATAAAATGCATGCTGTAGTAGCTAGCACTGCATCTATCTATGTATATGAGTTTGCGTCTTCTTTGAATCATAAATATGTGTGTAATAAATATCGTGCATAACGTATTAGTTATGCACTTTATCATATGGAATAAAAATATTATTAATCGATAATTAAACTTTAAAGTTNRRAGTTT
TARGET=193,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02858 Ps_contig_mira-and-tgicl-ass_franssen_25358
SEQUENCE=TATTGATCTTTTATTTGTCTCTACACCCAACTAAGGTAACGGTTTCCTATCTTCTTCTTTTACTAAAGACGAAGGAATTGATCGGTTACTATTCGGTTCTTGGATCTGTATATCTGTANGTTTTTTCTACTAGATCTGANGTTTTTTGTTTGATTTCTGTTCAGATCATTAACGATCGTGAGACTGGAAGGTCAAGAGGTTTTGGATTCGTGACCTTTGCCACGGAGCAATCCATGAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGCGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAATTGAAAAAATGTGAGATTARTGGTTACAGNTTTTG
TARGET=327,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02859 Ps_contig_mira-and-tgicl-ass_franssen_25382
SEQUENCE=CGGGTAAACCTTACGGGTAAGTTCAAACCCCTACGCCTAGGAGCTTGGTGCTGCAATTATCTTCACAAAGATCTTGGTTGGGATGATCAATGGATTTTCTGGGTTGGACCATTCATTGGTGCAGCACTTGCAGCACTTTACCACACAGTTGTTATCAGAGCCATTCCTTTCAAGTCAAGTTGATATGATCCAACGGTTTGTGATAGTTGATGCCAAAACCATTAAACCAAACCAACTTTGTGGGGTTTTATTTTAATTGAAATTCGATCATTATCTTGTTTAACTATTTCCTTTTGTATGTAAGATGTTGTTGTAGTGTACGTGTTGTTGTATGATTATTATTATTAGGTGTGNGTGTGYGNGGTGGGNGTTTTCNTGNTTTAGTGATCNGCC
TARGET=335,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02860 Ps_contig_mira-and-tgicl-ass_franssen_25399
SEQUENCE=AGAAATAATTATTAGAAAAGCTTTTCAACCTTGATGACATGCAAAATGGTAGACCCGATCCCAACATCTAGAACCATTAATTTAAACTTCCGTTTTTTACCAGTTCCAGTCACACCCATTCCATTGGTTTAAGCTTCAGGATTCTCAACTGGAACTTCCTCTGCTGCTGGAGCTTCCACTGATTCAACTTGTTTTGTTCTCAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCTATTTTCAGTTAGTTGCTTATTGGCTCCTCCACTACTGCCTTCCGCNYCTTCCTYTCYTCTCACTTTCTRYCAACCGTATTCCT
TARGET=223,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02861 Ps_contig_mira-and-tgicl-ass_franssen_25413
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACCGAGAAGTACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTGTGTACACTTCTAACCG
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02862 Ps_contig_mira-and-tgicl-ass_franssen_25422
SEQUENCE=CACCATTGACAACGTCAAGGCCAAGATCCAAGACAAGGAAGGAATCCCACCTGACCAGCAGAGGTTGATCTTTGCCGGTAAGCAGTTGGAAGATGGAAGGACTCTTGCGGATTACAATATCCAGAGGGAGTCTACCCTCCATCTTGTGCTGCGTCTTCGTGGTGGTATGCAGATATTCGTGAAGACCTTGACTGGTAAGACCATCACCCTGGAGGTGGAGAACTCTGATACTATTGATAATGTGAAGGCGAAAATTCAGGACAAGGAAGGTATCCCACCGGATCAGCAGAGGTTGATCTTTGCCGGGAAGCAGTTGGAGGATGGAAGGACCCTTGCTGACTACAACATTCAGAAGGAGACGTTACCCTTCACCTTGTGCCTCCGTTCTTCGGTGGGTTGGGTTTTAGAGTCTTGGTTTTGTTGTTATGTTGTTGTTGT
TARGET=427,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_02863 Ps_contig_mira-and-tgicl-ass_franssen_25437
SEQUENCE=TTGCTTAAGTCAAATGATCCTGTCGGGGTTTAACCCTTTGTTTGTATGTTTTGGCTTGGGGTTCTATAGGTCATATTGTTGCTTATTATATCTTGGCCACTTCTAGCAATGGTTATGATCCTAGTTTCTTTGGTTAATTAAGATTACTATGTTGTTCGTTTATGTTGTTTAATTTGTCTTTTCTTGTCCCTTTTATGTTTATTAAGATGATGATGATGATGATGATGATAGTATTAGTTTATTTGTATGAGATATTCAACTAAGTTAACGTT
TARGET=206,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02864 Ps_contig_mira-and-tgicl-ass_franssen_25472
SEQUENCE=AAGCAGTGGTATCAACGCAGAGTAAGTTTGGCTTTAGGCTTATTGATTCAGTGTTTTGAATGGACACGTATAGGTAAAGAAAAAATTGACATGACCGAAGGAAAAGGAATCACTGTGGGAAAAAAGATTTCCTTAAAAGTTAAGTGCAAATTACGTCATCCAGTGAAGATTAAATTTGCCTTCTAATTTATGAATGTTTGACATTTCTTAATAAGAGATACATGACGTAATCATCTTCAAATAATTTTTAAGTATGTAATTTCAAGATGATTTCAAGAATGTTTTTTAATTAATTAATATTTAGTTTTGGTGGAAAGTATAGATTTTTTTATATATATATTTANATTATACATTAAAAATTACG
TARGET=286,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02865 Ps_contig_mira-and-tgicl-ass_franssen_25472
SEQUENCE=AAGCAGTGGTATCAACGCAGAGTAAGTTTGGCTTTAGGCTTATTGATTCAGTGTTTTGAATGGACACGTATAGGTAAAGAAAAAATTGACATGACCGAAGGAAAAGGAATCACTGTGGGAAAAAAGATTTCCTTAAAAGTTAAGTGCAAATTACGTCATCCAGTGAAGATTAAATTTGCCTTCTAATTTATGAATGTTTGACATTTCTTAATAAGAGATACATGACGTAATCATCTTCAAATAATTTTTAAGTATGTAATTTCAAGATGATTTCAAGAATGTTTTTTAATTAATTAATATTTAGTTTTGGTGGAAAGTATAGATTTTTTTATATATATATTTANATTATACATTAAAAATTACG
TARGET=330,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02866 Ps_contig_mira-and-tgicl-ass_franssen_25485
SEQUENCE=AACGCAGTGGTATCAACCGACAGAGTACGCGGGGTGTCTGCGCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCGGACCTTTGCCGGAGAATGGTCTCGGCAAGCTCCGAAAACACCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGTATTATTTACCGTTGGCGCTGGTGAGTTTTGATCAGAATCAGAGGGTGGAAACGTGAGATCCTACGGTGGGGATTCGTTAGAGGGTGCAGTGAAAGTGACACGTAGTATCATGATAGTGAAACCAGCTGGGTACCAGAGTCCAAGTGGATCAGCTCCGGCTTCCCCCGCTGGTTCTACTCCGCCGCTATCT
TARGET=138,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1385
PRIMER_LEFT_PENALTY=0.052187
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=TGGTATCAACCGACAGAGTACG
PRIMER_RIGHT_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT=7,22
PRIMER_RIGHT=269,22
PRIMER_LEFT_TM=60.052
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.1604
PRIMER_LEFT_1_PENALTY=0.052187
PRIMER_RIGHT_1_PENALTY=0.108206
PRIMER_LEFT_1_SEQUENCE=TGGTATCAACCGACAGAGTACG
PRIMER_RIGHT_1_SEQUENCE=ACCCAGCTGGTTTCACTATCAT
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=302,22
PRIMER_LEFT_1_TM=60.052
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=0.3621
PRIMER_LEFT_2_PENALTY=0.275745
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_2_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=269,22
PRIMER_LEFT_2_TM=60.276
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.3840
PRIMER_LEFT_3_PENALTY=0.275745
PRIMER_RIGHT_3_PENALTY=0.108206
PRIMER_LEFT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_3_SEQUENCE=ACCCAGCTGGTTTCACTATCAT
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=302,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.5151
PRIMER_LEFT_4_PENALTY=0.052187
PRIMER_RIGHT_4_PENALTY=0.462913
PRIMER_LEFT_4_SEQUENCE=TGGTATCAACCGACAGAGTACG
PRIMER_RIGHT_4_SEQUENCE=TACCTTGTCGGAGATAGATCGG
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=172,22
PRIMER_LEFT_4_TM=60.052
PRIMER_RIGHT_4_TM=60.463
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_02867 Ps_contig_mira-and-tgicl-ass_franssen_25495
SEQUENCE=AGNACTCGAACGGAATCCCGCTACAGGCTAGTTGCTTCAAATACCCTCACACACCGTGTCCTCGGGGACTCAAACTAAACACACACAGTCTCTCTCTCTTTTCACCTCTCGCGTCCCTCTTTCTCTCGGGCTCTCACTGCGTCAAAAACCACGAGAAGAGGAACAACATGCAGATCTTCGTCAAGACTTTAACCGGAAAAACCATCACTCTCGAGGTTGAGAGCAGCGACACCATCGACAACGTCAAGGCCAAGATCCAGGACAAGGAAGGTATCCCTCCTGACCAGCAGAGGTTGTATTTTTGCTGGTAAGCAGCTGGAAGATGGTCGCACTCTTGCTGATTACAACATTACAAAAGGAATCGACGTCTTCTACTTGGTCCTGAGGCTCGAAGGGGAGTACCATAGTAATTAAAGTTAACG
TARGET=89,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0712
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.038090
PRIMER_LEFT_SEQUENCE=CTCGGGGACTCAAACTAAACAC
PRIMER_RIGHT_SEQUENCE=ATGTTGTTCCTCTTCTCGTGGT
PRIMER_LEFT=60,22
PRIMER_RIGHT=168,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=0.0754
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.038090
PRIMER_LEFT_1_SEQUENCE=TGCTTCAAATACCCTCACACAC
PRIMER_RIGHT_1_SEQUENCE=ATGTTGTTCCTCTTCTCGTGGT
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=168,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=60.038
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=0.1513
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.118120
PRIMER_LEFT_2_SEQUENCE=CTCGGGGACTCAAACTAAACAC
PRIMER_RIGHT_2_SEQUENCE=TCTTGACGAAGATCTGCATGTT
PRIMER_LEFT_2=60,22
PRIMER_RIGHT_2=185,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.882
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.1555
PRIMER_LEFT_3_PENALTY=0.037339
PRIMER_RIGHT_3_PENALTY=0.118120
PRIMER_LEFT_3_SEQUENCE=TGCTTCAAATACCCTCACACAC
PRIMER_RIGHT_3_SEQUENCE=TCTTGACGAAGATCTGCATGTT
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=59.882
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.1617
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.128591
PRIMER_LEFT_4_SEQUENCE=CTCGGGGACTCAAACTAAACAC
PRIMER_RIGHT_4_SEQUENCE=AGAGTGATGGTTTTTCCGGTTA
PRIMER_LEFT_4=60,22
PRIMER_RIGHT_4=210,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=59.871
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_02868 Ps_contig_mira-and-tgicl-ass_franssen_25518
SEQUENCE=AATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCTATGGGCTTCCAGAGGAGGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGCTTGATGGGTTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGTGTATTATATTGACAATAGAAATTAGAAAAGTATTTAGTTATGTTCTTTTAGTAA
TARGET=173,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2954
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.283237
PRIMER_LEFT_SEQUENCE=TCCAGAGGAGGCTACTTTCTTG
PRIMER_RIGHT_SEQUENCE=TCAATATAATACACGATGGCCG
PRIMER_LEFT=45,22
PRIMER_RIGHT=209,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=59.717
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=0.5852
PRIMER_LEFT_1_PENALTY=0.301926
PRIMER_RIGHT_1_PENALTY=0.283237
PRIMER_LEFT_1_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_1_SEQUENCE=TCAATATAATACACGATGGCCG
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=209,22
PRIMER_LEFT_1_TM=60.302
PRIMER_RIGHT_1_TM=59.717
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=148
PRIMER_PAIR_PENALTY_2=0.5852
PRIMER_LEFT_2_PENALTY=0.301926
PRIMER_RIGHT_2_PENALTY=0.283237
PRIMER_LEFT_2_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_2_SEQUENCE=TCAATATAATACACGATGGCCG
PRIMER_LEFT_2=63,22
PRIMER_RIGHT_2=209,22
PRIMER_LEFT_2_TM=60.302
PRIMER_RIGHT_2_TM=59.717
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=0.6304
PRIMER_LEFT_3_PENALTY=0.347129
PRIMER_RIGHT_3_PENALTY=0.283237
PRIMER_LEFT_3_SEQUENCE=GGAGGCTACTTTCTTGGTGGTA
PRIMER_RIGHT_3_SEQUENCE=TCAATATAATACACGATGGCCG
PRIMER_LEFT_3=51,22
PRIMER_RIGHT_3=209,22
PRIMER_LEFT_3_TM=59.653
PRIMER_RIGHT_3_TM=59.717
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=12.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=0.8109
PRIMER_LEFT_4_PENALTY=0.527639
PRIMER_RIGHT_4_PENALTY=0.283237
PRIMER_LEFT_4_SEQUENCE=AGGAGGCTACTTTCTTGGTGGT
PRIMER_RIGHT_4_SEQUENCE=TCAATATAATACACGATGGCCG
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=209,22
PRIMER_LEFT_4_TM=60.528
PRIMER_RIGHT_4_TM=59.717
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=12.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_02869 Ps_contig_mira-and-tgicl-ass_franssen_25553
SEQUENCE=CGTCGTTCCTTTCCTTTCCTTCTATCTGGTTCTAGCCTTTAAAACTTAGGTTTGCNCTTTTTTCCGTTAACGTAAAGAAGGCGGCTCCGGTCGAAGCAGAAGGTGGTTGCTCCGGCCAACGAGGAACTCGCCAAGTGGTATGGTCCGTGACAGAAGGATCTTCTTGCCTGATGGTCTCTTGGACAGGTCCGAGATTCCCGAGTACTTGACCGGAGAAGTCCCCGGAGACTATGGTTATGATCCTTTTGGTCTAAGCAAGAAGCCTGAAGACTTTGCCAAAT
TARGET=6,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02870 Ps_contig_mira-and-tgicl-ass_franssen_25567
SEQUENCE=TGTTTGGTAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTNTTATGTGTTGNGAGTTCTTTTTTATGTGTACTACTTA
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02871 Ps_contig_mira-and-tgicl-ass_franssen_25573
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAATTGAGGGATACGTTCTTCAATGGCGAAACNGAGAAATCTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATGAAAGAAAGAAATNTAATTGGTGAGATTACAGTATCTCTTATGTGTTGTGACTTTCTTTTTTTTACTGGTAGTACCTACCTTT
TARGET=155,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02872 Ps_contig_mira-and-tgicl-ass_franssen_25590
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTTGTTGTGAGTCT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02873 Ps_contig_mira-and-tgicl-ass_franssen_25653
SEQUENCE=AAGCAGTGGTATCAACGCTAGAGTTTGTGTTGGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATCTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTTGGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTTCTTTTTTTACTGTGTACACTTCTAACCCG
TARGET=185,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02874 Ps_contig_mira-and-tgicl-ass_franssen_25659
SEQUENCE=GTACAAAACAACAGAAAACTTTTGTTTCATCCACTAATTTTTTAACTAAAAACATTATTTCAGAATGCATCATTGGTCACACATAAAGAAGACTAATACCAGTTATAAATAAATAAAAAGTTTTACCTCCCAAAATTGGAGAACTATACTGTTCAAGGGTCATTGATCTCTGTCGTGTGTCAGATCTATGACCAGTTCAGAAGTTTTAAAGTTCTTAAATACTAAAAATAAGAAATGTGTCTGAAACATGTAAAACTGTTTCAAGGCTCCGGTTGCATGTTCTCACACTCTTCTCGAATTTTCTGGAAAAGAACAGTTGACAGATATCTTTCGCCAAAGCTCGGGAATACAACCGCAATAAGCTTTCCCTTATTTTCAGGTCTCTTAGCCACCTGCAATGCTGCAGCAGCAGCAGCACCTGAAGAAATTCCCACCAGCAAGCCTTCTTTGAGTGCTATTTGCTTTGTAGTTTCAATAGCTACATCACTAGATATCGCTATAACTTCATCAACCACTTCTTCATCCAAATTCCCGGGCACAAAACCAGCCCCAATACCCTGAATTTTGTGAGGTCCTGGCTTTCCACCTGAGAGTATGTTGCTTTCAAGAGGCTCTACACCAATAACCTGTACTTTTGAATTTTGTTGTTTTAGGAATCTACCGGCTCCAGAAATAGTTCCACCAGTTCCTATTCCTGCAACAAAAATATCTATTTTACCTCTTGTATCTTCCCATATCTCTGGCCCAGTTGTCTCAAAATGTATCTTAGGATTTGAAGGGTTATCAAATTGTTGAAGCATGTATGCATCGGGCGTATTTTTTACAATCTCTTCAGCTTTTTGAACTGCTCCATTCATACCCTTCGCGGCTTCAGTCAAAACAAGCTCAGCTCCAAATGCTTTCAAAAGAACGCGTCTTTCCAAACTCATGGAAGCAGGCATCGTCAAAATGAGTTTATATCCTTTAGAAGCTGACTAATAAACGGCCAGACCTATTCCTGTCTCCATAGTACGTCCAACCAAACNCTCCTCCGTAGG
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6568
PRIMER_LEFT_PENALTY=0.655497
PRIMER_RIGHT_PENALTY=0.001315
PRIMER_LEFT_SEQUENCE=GAATGCATCATTGGTCACACAT
PRIMER_RIGHT_SEQUENCE=ACGACAGAGATCAATGACCCTT
PRIMER_LEFT=62,22
PRIMER_RIGHT=175,22
PRIMER_LEFT_TM=60.655
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=1.1887
PRIMER_LEFT_1_PENALTY=0.655497
PRIMER_RIGHT_1_PENALTY=0.533247
PRIMER_LEFT_1_SEQUENCE=GAATGCATCATTGGTCACACAT
PRIMER_RIGHT_1_SEQUENCE=TCTGACACACGACAGAGATCAA
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=183,22
PRIMER_LEFT_1_TM=60.655
PRIMER_RIGHT_1_TM=59.467
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=1.3694
PRIMER_LEFT_2_PENALTY=1.368077
PRIMER_RIGHT_2_PENALTY=0.001315
PRIMER_LEFT_2_SEQUENCE=AGAATGCATCATTGGTCACACA
PRIMER_RIGHT_2_SEQUENCE=ACGACAGAGATCAATGACCCTT
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=175,22
PRIMER_LEFT_2_TM=61.368
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=115
PRIMER_PAIR_PENALTY_3=1.3939
PRIMER_LEFT_3_PENALTY=1.392550
PRIMER_RIGHT_3_PENALTY=0.001315
PRIMER_LEFT_3_SEQUENCE=GAATGCATCATTGGTCACACA
PRIMER_RIGHT_3_SEQUENCE=ACGACAGAGATCAATGACCCTT
PRIMER_LEFT_3=62,21
PRIMER_RIGHT_3=175,22
PRIMER_LEFT_3_TM=60.393
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=1.4904
PRIMER_LEFT_4_PENALTY=0.655497
PRIMER_RIGHT_4_PENALTY=0.834931
PRIMER_LEFT_4_SEQUENCE=GAATGCATCATTGGTCACACAT
PRIMER_RIGHT_4_SEQUENCE=TGGTCATAGATCTGACACACGA
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=193,22
PRIMER_LEFT_4_TM=60.655
PRIMER_RIGHT_4_TM=59.165
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_02875 Ps_contig_mira-and-tgicl-ass_franssen_25659
SEQUENCE=GTACAAAACAACAGAAAACTTTTGTTTCATCCACTAATTTTTTAACTAAAAACATTATTTCAGAATGCATCATTGGTCACACATAAAGAAGACTAATACCAGTTATAAATAAATAAAAAGTTTTACCTCCCAAAATTGGAGAACTATACTGTTCAAGGGTCATTGATCTCTGTCGTGTGTCAGATCTATGACCAGTTCAGAAGTTTTAAAGTTCTTAAATACTAAAAATAAGAAATGTGTCTGAAACATGTAAAACTGTTTCAAGGCTCCGGTTGCATGTTCTCACACTCTTCTCGAATTTTCTGGAAAAGAACAGTTGACAGATATCTTTCGCCAAAGCTCGGGAATACAACCGCAATAAGCTTTCCCTTATTTTCAGGTCTCTTAGCCACCTGCAATGCTGCAGCAGCAGCAGCACCTGAAGAAATTCCCACCAGCAAGCCTTCTTTGAGTGCTATTTGCTTTGTAGTTTCAATAGCTACATCACTAGATATCGCTATAACTTCATCAACCACTTCTTCATCCAAATTCCCGGGCACAAAACCAGCCCCAATACCCTGAATTTTGTGAGGTCCTGGCTTTCCACCTGAGAGTATGTTGCTTTCAAGAGGCTCTACACCAATAACCTGTACTTTTGAATTTTGTTGTTTTAGGAATCTACCGGCTCCAGAAATAGTTCCACCAGTTCCTATTCCTGCAACAAAAATATCTATTTTACCTCTTGTATCTTCCCATATCTCTGGCCCAGTTGTCTCAAAATGTATCTTAGGATTTGAAGGGTTATCAAATTGTTGAAGCATGTATGCATCGGGCGTATTTTTTACAATCTCTTCAGCTTTTTGAACTGCTCCATTCATACCCTTCGCGGCTTCAGTCAAAACAAGCTCAGCTCCAAATGCTTTCAAAAGAACGCGTCTTTCCAAACTCATGGAAGCAGGCATCGTCAAAATGAGTTTATATCCTTTAGAAGCTGACTAATAAACGGCCAGACCTATTCCTGTCTCCATAGTACGTCCAACCAAACNCTCCTCCGTAGG
TARGET=403,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0874
PRIMER_LEFT_PENALTY=0.058146
PRIMER_RIGHT_PENALTY=0.029253
PRIMER_LEFT_SEQUENCE=GCATGTTCTCACACTCTTCTCG
PRIMER_RIGHT_SEQUENCE=TTGGATGAAGAAGTGGTTGATG
PRIMER_LEFT=274,22
PRIMER_RIGHT=526,22
PRIMER_LEFT_TM=60.058
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.1063
PRIMER_LEFT_1_PENALTY=0.058146
PRIMER_RIGHT_1_PENALTY=0.048131
PRIMER_LEFT_1_SEQUENCE=GCATGTTCTCACACTCTTCTCG
PRIMER_RIGHT_1_SEQUENCE=ATAGCACTCAAAGAAGGCTTGC
PRIMER_LEFT_1=274,22
PRIMER_RIGHT_1=457,22
PRIMER_LEFT_1_TM=60.058
PRIMER_RIGHT_1_TM=60.048
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.1915
PRIMER_LEFT_2_PENALTY=0.162217
PRIMER_RIGHT_2_PENALTY=0.029253
PRIMER_LEFT_2_SEQUENCE=GTAAAACTGTTTCAAGGCTCCG
PRIMER_RIGHT_2_SEQUENCE=TTGGATGAAGAAGTGGTTGATG
PRIMER_LEFT_2=249,22
PRIMER_RIGHT_2=526,22
PRIMER_LEFT_2_TM=60.162
PRIMER_RIGHT_2_TM=59.971
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.2103
PRIMER_LEFT_3_PENALTY=0.162217
PRIMER_RIGHT_3_PENALTY=0.048131
PRIMER_LEFT_3_SEQUENCE=GTAAAACTGTTTCAAGGCTCCG
PRIMER_RIGHT_3_SEQUENCE=ATAGCACTCAAAGAAGGCTTGC
PRIMER_LEFT_3=249,22
PRIMER_RIGHT_3=457,22
PRIMER_LEFT_3_TM=60.162
PRIMER_RIGHT_3_TM=60.048
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.2968
PRIMER_LEFT_4_PENALTY=0.248690
PRIMER_RIGHT_4_PENALTY=0.048131
PRIMER_LEFT_4_SEQUENCE=CATTGATCTCTGTCGTGTGTCA
PRIMER_RIGHT_4_SEQUENCE=ATAGCACTCAAAGAAGGCTTGC
PRIMER_LEFT_4=160,22
PRIMER_RIGHT_4=457,22
PRIMER_LEFT_4_TM=59.751
PRIMER_RIGHT_4_TM=60.048
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_02876 Ps_contig_mira-and-tgicl-ass_franssen_25716
SEQUENCE=TTACCCTACCAAAACCTAAACTTAGGAATTGGCCTGGGGTTCCGAAAGGAAAACCCAAACCGRAACCAACNGAACCAAAACCCTACCTTAAANCCCTTTACGGTCCATCTGAACAAACCAATCTATCCAAGCTCTGATCCATGGCTTGAACCGACATTATTACTCAATAGCAATCAATTACAGGAAGAACGAGCTTGAAGAGAAGATGCTGCTTAACCTTCATAAGAAGAAATGGACCGATGGTTTGACACTCAGACGATTTGATTCACATTCTAAAACTAATGAACAGACTGTTCAGGAGATGCTAAGTTTAGCAACCAAGTACAACAAGGCAGTGCAAGAGGAAGATGAGCTGCCCCCAGAAAAGCTTGCAATTGCTAATGTGGGAAGGCAGGACGCTAAGAAGCATCTTGAAGAGCACGTTTCTAATTTAATGTCTTCGAACATTGTTCAGACTTTGGGAATGATGCTCGACACAGTTGTGTTCTAGAGTTAGGATCGTCCACTGTATTCTTCTTCTTCTTCTTCATGATGTCCAGCAATATTTCATTTACACTCTTTGACCTGTTTGCAATAGATTGTTTTTTATTACAAAGTTATTTGCATTTCATTGTGTTCGTCTCAAATGTTTCCGGATGATAATCATTTTCTTAAGCTTTAGAGACGACGGGGGACTCTGCTTAAAAATCATAGAATGCATTTGTATTTTTAGTTTTTAAAAAAAAAGTTATAGTCCTTAATCAAGTCTTACTCCCTCTTGACCATTAACCTTCTA
TARGET=511,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02877 Ps_contig_mira-and-tgicl-ass_franssen_25722
SEQUENCE=CTAAAACGGAGTACATACAAAAAAACAACCTCGTATTTTATTTGTGCTATAGTATAATTTTATTGTTGTTAACTATATAACTACTAATTAGTTATTACATGATAATGAAAAACCTTTCAAGTTTTCCTTTTTCTTTATATTCTCACAAAAAACTCATCTTCATCTTCAAAGTTTTTACTATGTCAAACAAGGGTTTGATGGATGATAGAGATATAGAGAGAGGTTTTGATGGGTTAAAGAAGAAAAGAAGAAGATTGTTATGGTNATTCCTTGTCGGAGATAGATCGGGAAAGGAAGGAAGGGTGTTTTCGGAGCTTGCCGAGACCATTCTCCGGCAAAGGTCCGGCGACGGTGTCATCCCACAAGTGATCGAGAAGGCTCATGAGAATATGGAAAGCGCAGACA
TARGET=291,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3431
PRIMER_LEFT_PENALTY=0.067353
PRIMER_RIGHT_PENALTY=0.275745
PRIMER_LEFT_SEQUENCE=TGGTNATTCCTTGTCGGAGATA
PRIMER_RIGHT_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT=260,22
PRIMER_RIGHT=382,22
PRIMER_LEFT_TM=60.067
PRIMER_RIGHT_TM=60.276
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=0.3431
PRIMER_LEFT_1_PENALTY=0.067353
PRIMER_RIGHT_1_PENALTY=0.275745
PRIMER_LEFT_1_SEQUENCE=TATGGTNATTCCTTGTCGGAGA
PRIMER_RIGHT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_1=258,22
PRIMER_RIGHT_1=382,22
PRIMER_LEFT_1_TM=60.067
PRIMER_RIGHT_1_TM=60.276
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=0.5938
PRIMER_LEFT_2_PENALTY=0.318073
PRIMER_RIGHT_2_PENALTY=0.275745
PRIMER_LEFT_2_SEQUENCE=ATGGTNATTCCTTGTCGGAGAT
PRIMER_RIGHT_2_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_2=259,22
PRIMER_RIGHT_2=382,22
PRIMER_LEFT_2_TM=60.318
PRIMER_RIGHT_2_TM=60.276
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=0.6220
PRIMER_LEFT_3_PENALTY=0.346220
PRIMER_RIGHT_3_PENALTY=0.275745
PRIMER_LEFT_3_SEQUENCE=TGTTATGGTNATTCCTTGTCGG
PRIMER_RIGHT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_3=255,22
PRIMER_RIGHT_3=382,22
PRIMER_LEFT_3_TM=60.346
PRIMER_RIGHT_3_TM=60.276
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.75
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=128
PRIMER_PAIR_PENALTY_4=0.6910
PRIMER_LEFT_4_PENALTY=0.415257
PRIMER_RIGHT_4_PENALTY=0.275745
PRIMER_LEFT_4_SEQUENCE=TTATGGTNATTCCTTGTCGGAG
PRIMER_RIGHT_4_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_4=257,22
PRIMER_RIGHT_4=382,22
PRIMER_LEFT_4_TM=59.585
PRIMER_RIGHT_4_TM=60.276
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_02878 Ps_contig_mira-and-tgicl-ass_franssen_25753
SEQUENCE=AGTTTTTTTCTTTCTCAAAAATGGATCTTTTGAAATTTGGTTTATGTACGCGATATGAGAGACAAATAATTAAAAACTTGAAACAAATAAGACAAAGAAAGAAAACAATACTCTAAATGTAGCTTTGATGCATATAAATAAACATTAGCAGAGAGTGAAAGAAACTTCAATGAAGAATGAAACACAAAAATGGAAACAATGTCATCTAAACAAGTTACAAAATTACAACACCTCGTCTTCAAGAAGTGTCGGTTAGTGTATGCTCTTCTTCTTCTGTCACAAGAAGCTTTCCTTCGACAAAATTACAGCATGCTATCGGTAATTCAAGTTGCTTCTTCAATTTTCTCACAACAAAAACCTCTGACTCTTCACATATGGTTAGCACTGTACCGTTCCTACCAAGCCGACCGGTTCGACCGGCTCGATGTGCATAGTGAATTGAATCTGTCGGCAATTCGAGGTTAACCACAAGATCACATTCTGCTACATCCAAACCCCTTGCTGATAATTCATTTGTCACAAGAACTCTAACCTCGCCGTTCTTGAATTTCTTCAGAGTTGTCGACCTGCCAAGCTTCCCGAGATCTCCGTGTAACTCTGCGTTGATACCACTGCTT
TARGET=264,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8293
PRIMER_LEFT_PENALTY=0.580184
PRIMER_RIGHT_PENALTY=0.249074
PRIMER_LEFT_SEQUENCE=CGTCTTCAAGAAGTGTCGGTTA
PRIMER_RIGHT_SEQUENCE=GGGTTTGGATGTAGCAGAATGT
PRIMER_LEFT=233,22
PRIMER_RIGHT=496,22
PRIMER_LEFT_TM=59.420
PRIMER_RIGHT_TM=60.249
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.8828
PRIMER_LEFT_1_PENALTY=0.580184
PRIMER_RIGHT_1_PENALTY=0.302618
PRIMER_LEFT_1_SEQUENCE=CGTCTTCAAGAAGTGTCGGTTA
PRIMER_RIGHT_1_SEQUENCE=GGAACGGTACAGTGCTAACCAT
PRIMER_LEFT_1=233,22
PRIMER_RIGHT_1=395,22
PRIMER_LEFT_1_TM=59.420
PRIMER_RIGHT_1_TM=60.303
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=1.0814
PRIMER_LEFT_2_PENALTY=0.580184
PRIMER_RIGHT_2_PENALTY=0.501224
PRIMER_LEFT_2_SEQUENCE=CGTCTTCAAGAAGTGTCGGTTA
PRIMER_RIGHT_2_SEQUENCE=GGTTTGGATGTAGCAGAATGTG
PRIMER_LEFT_2=233,22
PRIMER_RIGHT_2=495,22
PRIMER_LEFT_2_TM=59.420
PRIMER_RIGHT_2_TM=59.499
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=1.0955
PRIMER_LEFT_3_PENALTY=0.846446
PRIMER_RIGHT_3_PENALTY=0.249074
PRIMER_LEFT_3_SEQUENCE=CTCGTCTTCAAGAAGTGTCGGT
PRIMER_RIGHT_3_SEQUENCE=GGGTTTGGATGTAGCAGAATGT
PRIMER_LEFT_3=231,22
PRIMER_RIGHT_3=496,22
PRIMER_LEFT_3_TM=60.846
PRIMER_RIGHT_3_TM=60.249
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=1.0955
PRIMER_LEFT_4_PENALTY=0.846446
PRIMER_RIGHT_4_PENALTY=0.249074
PRIMER_LEFT_4_SEQUENCE=ACCTCGTCTTCAAGAAGTGTCG
PRIMER_RIGHT_4_SEQUENCE=GGGTTTGGATGTAGCAGAATGT
PRIMER_LEFT_4=229,22
PRIMER_RIGHT_4=496,22
PRIMER_LEFT_4_TM=60.846
PRIMER_RIGHT_4_TM=60.249
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_02879 Ps_contig_mira-and-tgicl-ass_franssen_25758
SEQUENCE=GGTGGTCTGCGCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCGGACCTTTGCCGGAGAATGGTCTCGGCAAGCTCCGAAAACACCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGAATCAGAGGGTGGAAACGTGAGATCCTACGGTGGGGATTCGTTAGAGGGTGCAGTGAAAGTGACACGTAGTATCATGATAGTGAAACCAGCTGGGTACCAGA
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3621
PRIMER_LEFT_PENALTY=0.275745
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT=25,22
PRIMER_RIGHT=202,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.3840
PRIMER_LEFT_1_PENALTY=0.275745
PRIMER_RIGHT_1_PENALTY=0.108206
PRIMER_LEFT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_1_SEQUENCE=ACCCAGCTGGTTTCACTATCAT
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=235,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.6439
PRIMER_LEFT_2_PENALTY=0.275745
PRIMER_RIGHT_2_PENALTY=0.368190
PRIMER_LEFT_2_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_2_SEQUENCE=TACCCAGCTGGTTTCACTATCA
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=236,22
PRIMER_LEFT_2_TM=60.276
PRIMER_RIGHT_2_TM=59.632
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.6994
PRIMER_LEFT_3_PENALTY=0.275745
PRIMER_RIGHT_3_PENALTY=0.423703
PRIMER_LEFT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_3_SEQUENCE=ATTCCTTGTCGGAGATAGATCG
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=142,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.576
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=0.8604
PRIMER_LEFT_4_PENALTY=0.275745
PRIMER_RIGHT_4_PENALTY=0.584629
PRIMER_LEFT_4_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_4_SEQUENCE=GGTACCCAGCTGGTTTCACTAT
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=238,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=59.415
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_02880 Ps_contig_mira-and-tgicl-ass_franssen_25808
SEQUENCE=CTTAAACAAAATTAGAAAATACAAGNATTTTCTTTTGTTTGAAAAAACAACAAGATGGCCACAATAACACACTAACTATCCTTGGCCTCCTCCGCGGCTGTCTCAAAAGTCTCTCCTGGGACAAGCTCTGGAACATCATCATCATCCTCTTCTTGTGCTGTGGCGGCTGTGCCAGCACCTGCTTCAGGAACCTGCTTATTGAACTGCTCGGCTAACTTCTTCAGGTTTTCCAAATTATCTGGCCCTAACTGGTGGATAATGCTAGGAAGTATGTCCTGCAACTTCTTGGTTTGAGGAGCACCACTAACAACCCATGTATTGGCAGCAATGGATGCTTGAACTTTGGGGCTTAAGAACTGGATGACAACATCATCCTTAAAGATATTGACCTCCTCAATTTGAGGGATGGCATTCACTCCAATTCTCTTCAGGGTACTCTGAAGCCTTTTGTCATCTGTAGTTGTTGTCTTGTGGACAGCCTTCTTCTTTCTTCTGACTGTACCCTTTCCACCAGTTCGAACTGAACCGGCCATCTTCATCAACTTTTCACGATTCATCTTTGTGAAATCGCTGATTTTGGACACAGGGTAAGCCTCAACCGTAAGAAAAGAAAATAACCCCGAA
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2248
PRIMER_LEFT_PENALTY=0.143960
PRIMER_RIGHT_PENALTY=0.080887
PRIMER_LEFT_SEQUENCE=TCTCAAAAGTCTCTCCTGGGAC
PRIMER_RIGHT_SEQUENCE=GAAGTTAGCCGAGCAGTTCAAT
PRIMER_LEFT=100,22
PRIMER_RIGHT=218,22
PRIMER_LEFT_TM=59.856
PRIMER_RIGHT_TM=59.919
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.3004
PRIMER_LEFT_1_PENALTY=0.143960
PRIMER_RIGHT_1_PENALTY=0.156479
PRIMER_LEFT_1_SEQUENCE=TCTCAAAAGTCTCTCCTGGGAC
PRIMER_RIGHT_1_SEQUENCE=GCCAGATAATTTGGAAAACCTG
PRIMER_LEFT_1=100,22
PRIMER_RIGHT_1=242,22
PRIMER_LEFT_1_TM=59.856
PRIMER_RIGHT_1_TM=59.844
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.3131
PRIMER_LEFT_2_PENALTY=0.273230
PRIMER_RIGHT_2_PENALTY=0.039879
PRIMER_LEFT_2_SEQUENCE=CAAAAGTCTCTCCTGGGACAAG
PRIMER_RIGHT_2_SEQUENCE=AAACCAAGAAGTTGCAGGACAT
PRIMER_LEFT_2=103,22
PRIMER_RIGHT_2=291,22
PRIMER_LEFT_2_TM=60.273
PRIMER_RIGHT_2_TM=60.040
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.3315
PRIMER_LEFT_3_PENALTY=0.291669
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=CAAGATGGCCACAATAACACAC
PRIMER_RIGHT_3_SEQUENCE=AAACCAAGAAGTTGCAGGACAT
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=291,22
PRIMER_LEFT_3_TM=60.292
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.3315
PRIMER_LEFT_4_PENALTY=0.291669
PRIMER_RIGHT_4_PENALTY=0.039879
PRIMER_LEFT_4_SEQUENCE=ACAAGATGGCCACAATAACACA
PRIMER_RIGHT_4_SEQUENCE=AAACCAAGAAGTTGCAGGACAT
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.292
PRIMER_RIGHT_4_TM=60.040
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_02881 Ps_contig_mira-and-tgicl-ass_franssen_25815
SEQUENCE=GTACACAAGTAAAAAAGACTCACAACACATAAGAGATACTGTAATCTACAACCAATTATACTTTTCTTTCTTTCAATTAGTCTGTCTCAACACCTAATCCATACAACACACCAGAGTATTTCTCTGTTTCGCCATTGAAGAACGTATCCCTCAATTTTCTAAATGCACAAGCAGTGAGTAAACTATCCGATCCTGCTTGATGGCAAACACCAATCCTTTCAACTTCCAACA
TARGET=63,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02882 Ps_contig_mira-and-tgicl-ass_franssen_25829
SEQUENCE=CACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTTGCTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTTCACTTCTTCAACCGATTCCTCAGTTTCTTTAGGTGCTTCTAAGTTCTCTTCCTTTGCTTCCTCTGTTACCACTTCCTTGTTCTCAACTTCAAGTGGATCTTGGGTTTCGGGAGCATCTACAGCTTCTCCTGCTGCAGTTGTTTCTGTTGGTTTTTCGGTTGTTTCTTCTGTTGGTACTTCGGTGGCAGGAACTTCGGATTCTGGTTGTGCCGCAGCAGGGACTTCAGTGGCTGGTTGCTGTTCTTGAATTGTTTCCTCTGTCTTGATTATCGTCGGGTTGTTTGTTTCTATTTTTCTTGCTACTTGNTGTTGGTGGTGGNTTGGGTTGTNCNRCCTCAA
TARGET=19,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02883 Ps_contig_mira-and-tgicl-ass_franssen_25847
SEQUENCE=TTTTATAATAGGGGATATATTTATTTGGCTTGTCAGTCAGCGTGATACAAAAACCAAACTTTGAAAATTACAATTTATGCAAACAACTGCAGACACATGCATATTGGGGCAGTGCCTTACAGTTGTGGCTAATACAATAAAAACACAAATTAAATAAATAAAAGCAACCCTCTCAACAATATTAATCAAAGACTGACGAAATATATATGTGAAGAATCCCCTCGGTATTAGTTGTTCATGGCTTTCTCTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGRTAYTTGTAAGGCTTCTTRTGAGGAGT
TARGET=151,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02884 Ps_contig_mira-and-tgicl-ass_franssen_25847
SEQUENCE=TTTTATAATAGGGGATATATTTATTTGGCTTGTCAGTCAGCGTGATACAAAAACCAAACTTTGAAAATTACAATTTATGCAAACAACTGCAGACACATGCATATTGGGGCAGTGCCTTACAGTTGTGGCTAATACAATAAAAACACAAATTAAATAAATAAAAGCAACCCTCTCAACAATATTAATCAAAGACTGACGAAATATATATGTGAAGAATCCCCTCGGTATTAGTTGTTCATGGCTTTCTCTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGRTAYTTGTAAGGCTTCTTRTGAGGAGT
TARGET=367,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02885 Ps_contig_mira-and-tgicl-ass_franssen_25847
SEQUENCE=TTTTATAATAGGGGATATATTTATTTGGCTTGTCAGTCAGCGTGATACAAAAACCAAACTTTGAAAATTACAATTTATGCAAACAACTGCAGACACATGCATATTGGGGCAGTGCCTTACAGTTGTGGCTAATACAATAAAAACACAAATTAAATAAATAAAAGCAACCCTCTCAACAATATTAATCAAAGACTGACGAAATATATATGTGAAGAATCCCCTCGGTATTAGTTGTTCATGGCTTTCTCTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGRTAYTTGTAAGGCTTCTTRTGAGGAGT
TARGET=423,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02886 Ps_contig_mira-and-tgicl-ass_franssen_25847
SEQUENCE=TTTTATAATAGGGGATATATTTATTTGGCTTGTCAGTCAGCGTGATACAAAAACCAAACTTTGAAAATTACAATTTATGCAAACAACTGCAGACACATGCATATTGGGGCAGTGCCTTACAGTTGTGGCTAATACAATAAAAACACAAATTAAATAAATAAAAGCAACCCTCTCAACAATATTAATCAAAGACTGACGAAATATATATGTGAAGAATCCCCTCGGTATTAGTTGTTCATGGCTTTCTCTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGRTAYTTGTAAGGCTTCTTRTGAGGAGT
TARGET=471,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02887 Ps_contig_mira-and-tgicl-ass_franssen_25847
SEQUENCE=TTTTATAATAGGGGATATATTTATTTGGCTTGTCAGTCAGCGTGATACAAAAACCAAACTTTGAAAATTACAATTTATGCAAACAACTGCAGACACATGCATATTGGGGCAGTGCCTTACAGTTGTGGCTAATACAATAAAAACACAAATTAAATAAATAAAAGCAACCCTCTCAACAATATTAATCAAAGACTGACGAAATATATATGTGAAGAATCCCCTCGGTATTAGTTGTTCATGGCTTTCTCTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGRTAYTTGTAAGGCTTCTTRTGAGGAGT
TARGET=525,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02888 Ps_contig_mira-and-tgicl-ass_franssen_25847
SEQUENCE=TTTTATAATAGGGGATATATTTATTTGGCTTGTCAGTCAGCGTGATACAAAAACCAAACTTTGAAAATTACAATTTATGCAAACAACTGCAGACACATGCATATTGGGGCAGTGCCTTACAGTTGTGGCTAATACAATAAAAACACAAATTAAATAAATAAAAGCAACCCTCTCAACAATATTAATCAAAGACTGACGAAATATATATGTGAAGAATCCCCTCGGTATTAGTTGTTCATGGCTTTCTCTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGRTAYTTGTAAGGCTTCTTRTGAGGAGT
TARGET=566,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02889 Ps_contig_mira-and-tgicl-ass_franssen_25847
SEQUENCE=TTTTATAATAGGGGATATATTTATTTGGCTTGTCAGTCAGCGTGATACAAAAACCAAACTTTGAAAATTACAATTTATGCAAACAACTGCAGACACATGCATATTGGGGCAGTGCCTTACAGTTGTGGCTAATACAATAAAAACACAAATTAAATAAATAAAAGCAACCCTCTCAACAATATTAATCAAAGACTGACGAAATATATATGTGAAGAATCCCCTCGGTATTAGTTGTTCATGGCTTTCTCTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATGGGCTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAATAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGCGGGGAATGGTAGACTGGGTGAGGATGAGGGTAGGTATGCACTGGTGGTGGTGGGGGAGATGAGTACTTGTATGGTTTCTTATGTGGTGGTGGTGGGGAATGGTAGACAGGGTGAGGATGAGGGTAGGTGTGCACTGGTGGTGGTGGAGGAGATGGRTAYTTGTAAGGCTTCTTRTGAGGAGT
TARGET=618,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02890 Ps_contig_mira-and-tgicl-ass_franssen_25854
SEQUENCE=CGTTCGTCCTTCGTCCTYACCTNNCNCCTACGTNTCGTCTTTCGTTCTCTTNTTCTTTTTTCTTTTCCTTCTTCTTCTTTCTTTTCTCACCATCTTCTCCTTCCTCTTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGGTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCACCTTGTGACCAAACTCGTGGGCAATCGCCTCATCTTGAGCAGGCTTGTGTTCTTCATTCCTTTTTCTTACCACCGAGAAATACAAAACNCCTCTGTCCTTGATCTCTGT
TARGET=68,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02891 Ps_contig_mira-and-tgicl-ass_franssen_25889
SEQUENCE=GATCGACACATTAGATTGCCATTATCTTATCCGTCAGATAAGAGGCGATCGTGCTCCTCTCGACTCAATCCTATCGGTACTTCACCCTTCTTCTTCCCACCAATCACAACACAAACTAAACACCCTCATTTCATTTCACTCACCACACAACTATTTCTTACCTCACCCTTCTTCTTCCTCTTCTTCCTCCTCTACCCATACCAACAATGGCGTCTCTACAGCACACCACCGCTTCACTCCACTCCAAACACATCCCCAAAACCACAAACATCCTCACTCGAAAACCCATCCTCAACCTCTCCTTTTCAACCTTCTACTCCCCAAAACTCAAACTCAAACTCAAACTCCCCCTCACCAAAACCCGCCGCTCCACCGGCGGAGCCCTCGGAGCCAGAATGTCATCCCTAGCCGCCGGCAGCTACGCAGCCGCTTTAGCCGACTTAGCAAATTCAAACAACACCCTCGACGCAATAACCGCCGACTTCGACAAAATCGAACAACTTTTCTCTGACCCCAAGGTGTTCGACTATTTCTCCAGCCCAATAGTCGAAGATTCCACCAAACGCCAACTTATAGGAGAGTTCGCCACTACCTCAGGCTTCCAACCACACACACACAACTTCCTTAACGTCTTGATCGACTCGAAGAGGATCGACATGATCATCGACATTATTAAGGAGTTTGAATTTGTTTACAATACTTTAACTGATACAGAGCTTGTGGTTGTGACTTCGGTTGTGAAGTTGGAGTCACATCATTTGGCTCAGATTGCAAAACAGGTTCAGAAGCTTACTGGGGCTAAGAAAGTGAGGACTAAAACACTTCTTGATCCAAGTTTGGTGGCTGGTTTTACTGTCAGGTATGGGAATACTGGCTCTAAGTTTATTGATATGAGTGTTAAGAGGAAACTCGAGGAAATTGCTGCTCAAATTGATTTGGGTGATATCCAACTTGCTGTATAAGCATTCTTCGTGCTTCCTTTCAATTTGGTTTACTCAAATTGTTAATTTTTTTTGGATTATAAGTATATTTATGTATCTATGTATGAATCCTCAAGTGTGTACAACCTTCTTACAATAATCATATTAATATTGAGTCCCTGCTTCTTAACTT
TARGET=607,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1152
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.103024
PRIMER_LEFT_SEQUENCE=AACAACTTTTCTCTGACCCCAA
PRIMER_RIGHT_SEQUENCE=ATCATGTCGATCCTCTTCGAGT
PRIMER_LEFT=495,22
PRIMER_RIGHT=660,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.103
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.1801
PRIMER_LEFT_1_PENALTY=0.093026
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=TAGGAGAGTTCGCCACTACCTC
PRIMER_RIGHT_1_SEQUENCE=TAGCCCCAGTAAGCTTCTGAAC
PRIMER_LEFT_1=573,22
PRIMER_RIGHT_1=800,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=0.2245
PRIMER_LEFT_2_PENALTY=0.137489
PRIMER_RIGHT_2_PENALTY=0.087031
PRIMER_LEFT_2_SEQUENCE=GTGTTCGACTATTTCTCCAGCC
PRIMER_RIGHT_2_SEQUENCE=TAGCCCCAGTAAGCTTCTGAAC
PRIMER_LEFT_2=518,22
PRIMER_RIGHT_2=800,22
PRIMER_LEFT_2_TM=60.137
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.2245
PRIMER_LEFT_3_PENALTY=0.137489
PRIMER_RIGHT_3_PENALTY=0.087031
PRIMER_LEFT_3_SEQUENCE=GGTGTTCGACTATTTCTCCAGC
PRIMER_RIGHT_3_SEQUENCE=TAGCCCCAGTAAGCTTCTGAAC
PRIMER_LEFT_3=517,22
PRIMER_RIGHT_3=800,22
PRIMER_LEFT_3_TM=60.137
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.2405
PRIMER_LEFT_4_PENALTY=0.137489
PRIMER_RIGHT_4_PENALTY=0.103024
PRIMER_LEFT_4_SEQUENCE=GTGTTCGACTATTTCTCCAGCC
PRIMER_RIGHT_4_SEQUENCE=ATCATGTCGATCCTCTTCGAGT
PRIMER_LEFT_4=518,22
PRIMER_RIGHT_4=660,22
PRIMER_LEFT_4_TM=60.137
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_02892 Ps_contig_mira-and-tgicl-ass_franssen_25935
SEQUENCE=TAGACAGTGGAGGAGGAGATATATTGTAAACTGGTGGTGGTGAAACCGGTGGAGGAGGAGATTATATTGTAATTGGCGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACTGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACCGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGATGGATTATTTGTTAGTGGAGGGAGGGCGGGTGGAGATGGTA
TARGET=332,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8448
PRIMER_LEFT_PENALTY=0.309369
PRIMER_RIGHT_PENALTY=0.535412
PRIMER_LEFT_SEQUENCE=TAGACACGTGGAGGAGGAGACT
PRIMER_RIGHT_SEQUENCE=AAATAATCCATCACCACCACCT
PRIMER_LEFT=266,22
PRIMER_RIGHT=382,22
PRIMER_LEFT_TM=60.309
PRIMER_RIGHT_TM=59.465
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=117
PRIMER_PAIR_PENALTY_1=0.8926
PRIMER_LEFT_1_PENALTY=0.260719
PRIMER_RIGHT_1_PENALTY=0.631910
PRIMER_LEFT_1_SEQUENCE=GACAGGTGGAGGAGGAGACTTA
PRIMER_RIGHT_1_SEQUENCE=CCCTCCCTCCACTAACAAATAA
PRIMER_LEFT_1=178,22
PRIMER_RIGHT_1=398,22
PRIMER_LEFT_1_TM=59.739
PRIMER_RIGHT_1_TM=59.368
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.9078
PRIMER_LEFT_2_PENALTY=0.372343
PRIMER_RIGHT_2_PENALTY=0.535412
PRIMER_LEFT_2_SEQUENCE=ATAGACACGTGGAGGAGGAGAC
PRIMER_RIGHT_2_SEQUENCE=AAATAATCCATCACCACCACCT
PRIMER_LEFT_2=265,22
PRIMER_RIGHT_2=382,22
PRIMER_LEFT_2_TM=59.628
PRIMER_RIGHT_2_TM=59.465
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=0.9288
PRIMER_LEFT_3_PENALTY=0.296849
PRIMER_RIGHT_3_PENALTY=0.631910
PRIMER_LEFT_3_SEQUENCE=TTTGTAAACTGGTGGTGGTGAA
PRIMER_RIGHT_3_SEQUENCE=CCCTCCCTCCACTAACAAATAA
PRIMER_LEFT_3=109,22
PRIMER_RIGHT_3=398,22
PRIMER_LEFT_3_TM=60.297
PRIMER_RIGHT_3_TM=59.368
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.9413
PRIMER_LEFT_4_PENALTY=0.309369
PRIMER_RIGHT_4_PENALTY=0.631910
PRIMER_LEFT_4_SEQUENCE=TAGACACGTGGAGGAGGAGACT
PRIMER_RIGHT_4_SEQUENCE=CCCTCCCTCCACTAACAAATAA
PRIMER_LEFT_4=266,22
PRIMER_RIGHT_4=398,22
PRIMER_LEFT_4_TM=60.309
PRIMER_RIGHT_4_TM=59.368
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_02893 Ps_contig_mira-and-tgicl-ass_franssen_25975
SEQUENCE=TAGTTTTTGTTATCAAACAAAATATATTACCAATAAAAAAGATACATAAGGATACAATACAATTAAGGATACATCTTTCGAAAAAAGAAACCAGTGAGAGACAATCCTTAACTATATATTCCTATCTCACCAATTATCTAAACACAATAATGAAAGACTTAATAAGTAACACGCACCCCTTGGTACCTAAGAATTTCACCAATAAGCTCTCCTTTCCCTGAACCAACATACCTTCCACAAATCTTCCCATCTCTTATGAACAAAAACGTAGGAACTTGAATCACTTTCATGTCTCCCAAAAACTGCATACAGCTTTCATTCTCATCCCCATTCATCCTCGCAAAAACAACCGTATCAATCATTTGTCTCGACAGCTTTATCACTGTCGGATAAACCTTAACACAAGGACCACAATGTTTCAATCCAACATCAAGTACGATAAGCTTATGATCAACCTTATGATCTTCAATAAGTTTCTCAACATCTTCTCTGTTATGAAGTTGCACCACAGCTGAATGGTTGTCACCGTAGTATAACACATCTCCAACGAGCATATCAGGACCAATAGCTTCTTCTTCGTGGATTTTTACTTTGCTTTTGTAGAAAGTGAAGTGAGGTACTTTATCTACTTTTTCTCTTTTGCAGAGTTCTTTTGTTTTCTCTGATTCGTCTCCCATGACGAAGATGAATTCAACGTCGTTGCAGGTTCGACTGAGGTCGACTAAGAAAGGGTATATCTCGGTGCTTTCTGGATTTTCTTTGGTGGCGTATTCGACTACTACGAGTTTGTCTTTTGCTGATACAAGTGCTTGGTCGAATTCTTCTATGCTGTGGACTTTTTGAACTCGTTCATCTTTTTTCTTTGCACCTGGAGCTGCTGTGGCTTTTGTGATGACAACACGAGACTTGATGGGAGAGAGTAGCGATGGAGGGTGGAAAATTGGTAGAGAAGAACGAAGGGAATGAAGGGAAAGGAACTTAGGGAGGGTTGTGATTGAAGGAGGGATTGAGGGTTTGCATATGAAATTGCTGCAGTGGGTGATTGAAGCCATGGATGAGAGATGTAAGGATTGGGAATGAAGAGAGAATTGAGTCGCCCGAAGAAGAAGAATATGAATGATAAAAAAGCATCCCCGA
TARGET=1100,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02894 Ps_contig_mira-and-tgicl-ass_franssen_25981
SEQUENCE=AGAACAAGGAARNAGGGTAACGAAGAGGAAAGGCAAAGGAAAGGAAGGAAACTTAAGTAAAGACACCTAAAGAAAACTAGAGGAAAACGGTTGAGAAGTGAAGGAAGAGGCGGAAGCAGTAGTGGAGGAGCCAATAGCAACTACTGAAAATGAGTCAGCACCACCACCACCAGCAACTGCAGAGGATGTGATTGAGAACAAAACAGTTGAATCAGTGGAAGCTCCAGCAGCAGAGGAAGTTCCAGTTGAGAATCCTGAAGCTTAAACCAATGGAATGGGTGTGACTGGAACTGGTAAAAAACGGAAGTTTAAATTAATGGTTCTAGATGTT
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02895 Ps_contig_mira-and-tgicl-ass_franssen_25982
SEQUENCE=AGGATGTTACACACTCTCACTCACCGCTATAAACCCCCAAAACCCTAACACTTTCNCGGGGAGACACGCTCTACCAGAACGAGGTTTGTCTCCTAGGGTTCGCACACAACAGACACTCTGTCTCTCATTCCGAACAACAACAACCATGGTGGCCGACAAGACCAAGAAGCTGAAGGTTTCTGAAAAGGGCGAGAACGCCGAGGAAATCGACGGAGAACTTGTCCTCTCCATTGAAAAGTTGCAGGAAATGCAAGATGAGCTTGAAAAGATCAATGAGGAGGCTAGTGATAAAGTTCTGGAAATTGAGCAGAAGTATAACGAGATAAGGAAGCCGCTGTATGATCAGCGCAATGATCTTATCAAGTCTATTCCTGATTTCTGGTTAACTGCTTTTTTGAGCCATCCTGCTCTTGGTGATCTTTTGAATGAGGAAGATCAGAAGATATTTAAGCATTTGAGTTCTCTTGAGGTTGAAGATCATAAAGATGTCAAATCGGGCTATTCAATCACCTTTAACTTTAATCCTAATCCCTATTTTGAGGATACAAAGCTTGTAAAGACTTTTACCTTCCTTGAAGAAGGAACAACAAAGGTTACTGCTACTCCCATTAAATGGAAAGAGGGCAAGGACATTCCAAATGGAGTTAGTCAAGAAAAGAAAGGCAACAAGCGAGCTGCTTCTGATGTCAGTTTCTTTACCTGGTTTAGTGATACCGAAGAGAAAGAAGATTTGGATGACCTCATTCATGATGAGGTTGCAGAATTAATCAAGGATGATTTATGGCCAAATCCACTTAATTATTTCAACAGCGAGGACCCTGACGAAGCGGAAGAGGATGATGATGAAGCTGGTGATGCGGGAAAGGATGATGACGACGACTCTGAAGATGATGATGACGATGACGACGATGATGATGAGGATGAATAAACAGTGTAAAATGCATTTACCTGGTTTTGTTATTTTTATTTTAAGGTTATTATAGGAGTATGTTAGTGGTTTTGTAACCTAGTTATTGTCCTTTGAAGTGGATTTACCTAGTTTTATCTCTAGTGCGCAATTTTTAAGACCAATGAGTGATAAGATCGGTTATAATGCAAGTATATTTAGTGTATAGTTGCGGACTCAACCACGTTTTGGTTTTTGGATATCTTGGTTTCAAGTTTGGAGCACTTAGAATTTATTGACATGTTCTTGAGTAGAATTGATTAAGTTTTATGTAATTGTTTCTATTTGAAAGTAGGATATATAGNTGTATCTCAGCGTGATTTTTATACTGAAATTTAGTATTTAATGAC
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1020
PRIMER_LEFT_PENALTY=0.023981
PRIMER_RIGHT_PENALTY=0.078029
PRIMER_LEFT_SEQUENCE=TTACACACTCTCACTCACCGCT
PRIMER_RIGHT_SEQUENCE=AACCTTCAGCTTCTTGGTCTTG
PRIMER_LEFT=6,22
PRIMER_RIGHT=177,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.922
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.1020
PRIMER_LEFT_1_PENALTY=0.023981
PRIMER_RIGHT_1_PENALTY=0.078029
PRIMER_LEFT_1_SEQUENCE=TTACACACTCTCACTCACCGCT
PRIMER_RIGHT_1_SEQUENCE=CAGAAACCTTCAGCTTCTTGGT
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.922
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=0.1406
PRIMER_LEFT_2_PENALTY=0.023981
PRIMER_RIGHT_2_PENALTY=0.116582
PRIMER_LEFT_2_SEQUENCE=TTACACACTCTCACTCACCGCT
PRIMER_RIGHT_2_SEQUENCE=GCAACTTTTCAATGGAGAGGAC
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=241,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.2317
PRIMER_LEFT_3_PENALTY=0.023981
PRIMER_RIGHT_3_PENALTY=0.207756
PRIMER_LEFT_3_SEQUENCE=TTACACACTCTCACTCACCGCT
PRIMER_RIGHT_3_SEQUENCE=AGCCTCCTCATTGATCTTTTCA
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=282,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=60.208
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.2389
PRIMER_LEFT_4_PENALTY=0.160828
PRIMER_RIGHT_4_PENALTY=0.078029
PRIMER_LEFT_4_SEQUENCE=GTTACACACTCTCACTCACCGC
PRIMER_RIGHT_4_SEQUENCE=AACCTTCAGCTTCTTGGTCTTG
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=177,22
PRIMER_LEFT_4_TM=59.839
PRIMER_RIGHT_4_TM=59.922
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_02896 Ps_contig_mira-and-tgicl-ass_franssen_25990
SEQUENCE=TTNATATATGGGATATGCTTTCATTTTGCATTAAGATGTTGATTATTGATAAGGTGAAATACAAAGATACCAAACCTCAAAGTTCATAACAAAAGGAACAGACATTTTATTACATTGGAAGCTAACATGTAATAAGAAGACAGCAAGAAAGCTAGAAAGGACAGTGATGATTGCATCATCAGTTGTATTAAGAAGACAGCAGAACACTTTATTGCATTGGAGGCTAACTTGTAGTATTAAGAAGACAGCAAGAAAGCTAGCTAGTAAAGAGAGTGATGATTGTATGATCAGTTGTAATTAGGATTGGCCAAAACGTAAGCCTCAATGGCCTTGAAAAGAGCATCACCCTTAGCTTTGCCTTCCTCAACTTCCTTTTCAATAGGCTTAGCATCTCCTTTTGTATGATATTTAACAATCATCTTACCAACAGATCCTCCATTTGGACCTTCAAACAATTTGGCCTCAAATGATATCTTCTCAACTATGTCTGATATACCGACACCTCCAACTATACTGTAATTATATTCAAACTTTGCATCATCAATGGCTTCAACTTTGTGCAACACGTACAAGGTCTGTCCTCCTTCAACAAAAGTGAGCTTCTTGACAGTGCCTGGACCACCATTTCCTTCAAGGATTTCAACAGTCTTGATTGAATCAACAACTTTTGGGACGATGAGATCAGCGTCATGCACGACAGCTTTGAAGAGCTTGGCAGGAGGGACGGTAGAGGTGGTATCATTCTCATATGTGAAAACACCCATGATTATTGATTTGATAAAAAGGAGTTTGAGAAGAAGATATGTATGTATGTTGGTGTATGTCCCCGACGTACTCTGCGTTGAG
TARGET=802,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1917
PRIMER_LEFT_PENALTY=0.129818
PRIMER_RIGHT_PENALTY=2.061873
PRIMER_LEFT_SEQUENCE=ACAACTTTTGGGACGATGAGAT
PRIMER_RIGHT_SEQUENCE=AGTACGTCGGGGACATACACC
PRIMER_LEFT=660,22
PRIMER_RIGHT=835,21
PRIMER_LEFT_TM=59.870
PRIMER_RIGHT_TM=61.062
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=57.143
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=2.1933
PRIMER_LEFT_1_PENALTY=0.131423
PRIMER_RIGHT_1_PENALTY=2.061873
PRIMER_LEFT_1_SEQUENCE=CTTTGTGCAACACGTACAAGGT
PRIMER_RIGHT_1_SEQUENCE=AGTACGTCGGGGACATACACC
PRIMER_LEFT_1=553,22
PRIMER_RIGHT_1=835,21
PRIMER_LEFT_1_TM=60.131
PRIMER_RIGHT_1_TM=61.062
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=57.143
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=2.1933
PRIMER_LEFT_2_PENALTY=0.131423
PRIMER_RIGHT_2_PENALTY=2.061873
PRIMER_LEFT_2_SEQUENCE=ACTTTGTGCAACACGTACAAGG
PRIMER_RIGHT_2_SEQUENCE=AGTACGTCGGGGACATACACC
PRIMER_LEFT_2=552,22
PRIMER_RIGHT_2=835,21
PRIMER_LEFT_2_TM=60.131
PRIMER_RIGHT_2_TM=61.062
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=57.143
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=284
PRIMER_PAIR_PENALTY_3=2.2023
PRIMER_LEFT_3_PENALTY=0.140441
PRIMER_RIGHT_3_PENALTY=2.061873
PRIMER_LEFT_3_SEQUENCE=GTGCAACACGTACAAGGTCTGT
PRIMER_RIGHT_3_SEQUENCE=AGTACGTCGGGGACATACACC
PRIMER_LEFT_3=557,22
PRIMER_RIGHT_3=835,21
PRIMER_LEFT_3_TM=60.140
PRIMER_RIGHT_3_TM=61.062
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=57.143
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=2.2076
PRIMER_LEFT_4_PENALTY=0.145769
PRIMER_RIGHT_4_PENALTY=2.061873
PRIMER_LEFT_4_SEQUENCE=AGGTCTGTCCTCCTTCAACAAA
PRIMER_RIGHT_4_SEQUENCE=AGTACGTCGGGGACATACACC
PRIMER_LEFT_4=571,22
PRIMER_RIGHT_4=835,21
PRIMER_LEFT_4_TM=60.146
PRIMER_RIGHT_4_TM=61.062
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=57.143
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_02897 Ps_contig_mira-and-tgicl-ass_franssen_26004
SEQUENCE=CTATTATTTTTATTTATACATATGCAAAGGGGAAACGGAAAGAAATACATACCTACCAGTACCACCCACCCATGATATAACACAACTTCAACTTTATCCGATTTTAAAGGATACTATAATAACATTGAAGGTGCGATACAATAATATTTCGGATACCGTAATAACATTGAAGGTACAATACAATAATATTTGTATTTTATCACTCAAACGATATCAAACAGTTCATGTTCATGAAGTTAATTATGATAATAGAATTATACGAGTACCACACACAGGCACAGCAAACTAAAGTTTTAAGGGCCACTATCAAGACCCGTCCAATAACAAATTCTAAAACTTAAAAACCAAAGACTTGTATAACACCCACTTATTATAAAACAGCAATGCAACATTCATATACTTAAAATCCACCGCGAAGGCGCAAAACAAGGTGAAGAGTAGACTCCTTCTGAATGTTGTAGTCAGCAAGAGTCCTTCCATCCTCCAATTGCTTCCCAGCAAATATCAACCTCTGCTGATCTGGTGGGATTCCCTCCTTGTCCTGTATTTTTGCCTTCACATTATCTATCGTGTCTGAACTTTCTACCTCCAAAGTAATGGTCTTTCCTGTCAAAGTCTTCACAAAGATCTGCATACCTCCCCTCAGACGAAGGACAAGATGAAGGGTGGATTCCTTCTGGATATTGTAATCAGCCAAGGTCCGTCCATCTTCAAGTTGTTTTCCAGCAAAAATCAATCTTTGCTGGTCTGGGGGAATGCCCTCCTTGTCCTGTATCTTAGCCTTCACATTATCAATTGTGTCTGAGCTCTCTACCTCCAAAGTAATTGTCTTGCCAGTAAGGGTCTTCACAAAAATCTGCATACCACCCCGAAGTCTCAAAACAAGATGAAGAGTGGACTCTTTCTGGATGTTGTAATCAGCCAGAGTTCTTCCATCTTCGAGCTGCTTTCCAGCAAAAATCAACCTTTGCTGATCTGGTGGAATACCCTCTTTGTCCTGTATCTTGGCCTTCACATTGTCTATGGTGTCAGAACTCTCCACCTCCAGGGTGATGGTCTTTCCTGTGAGGGTCTTGACAAAGATTTGCATACCACCACGGAGACGGAGCACCAGATGAAGGGTGGATTCTTTCTGGATGTTGTAGTCTGCTAAAGTTCTGCCATCCTCAAGTTGTTTGCCAGCAAAGATGAGCCTCTGTTGGTCAGGTGGAATGCCCTCTTTATCCTGAATCTTGGCTTTGACATTGTCAATGGTATCAGAGCTCTCCACCTCAAGGGTGATGGTCTTTCCAGTGAGAGTCTTCACGAAGATTTGCATCCCGCCACGGAGACGGAGCACCAAATGGAGAGTTGATTCCTTCTGTATATTGTAATCAGCAAGGGTACGCCCATCCTCTAGTTGCTTGCCAGCAAAAATGAGACGCTGCTGGTCTGGAGGGATTCCTTCCTTGTCTTGAATCTTGGCCTTAACATTGTCTATGGTATCTGAACTCTCAACTTCGAGAGTGATGGTCTTACCAGTGAGGGTCTTAACGAAGATTTGCATCTTGAAACGGTGTTGCGTGATATGTATGTATGAATCTCTTTGCTCTAAAACTAAATAACAACTTGTAGATGTCCCCGAC
TARGET=1567,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5054
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=2.501065
PRIMER_LEFT_SEQUENCE=AGCACCAAATGGAGAGTTGATT
PRIMER_RIGHT_SEQUENCE=CGGGGACATCTACAAGTTGTTATT
PRIMER_LEFT=1334,22
PRIMER_RIGHT=1622,24
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.501
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=2.5322
PRIMER_LEFT_1_PENALTY=0.031122
PRIMER_RIGHT_1_PENALTY=2.501065
PRIMER_LEFT_1_SEQUENCE=TGGTCTTACCAGTGAGGGTCTT
PRIMER_RIGHT_1_SEQUENCE=CGGGGACATCTACAAGTTGTTATT
PRIMER_LEFT_1=1509,22
PRIMER_RIGHT_1=1622,24
PRIMER_LEFT_1_TM=60.031
PRIMER_RIGHT_1_TM=60.501
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=2.6036
PRIMER_LEFT_2_PENALTY=0.102490
PRIMER_RIGHT_2_PENALTY=2.501065
PRIMER_LEFT_2_SEQUENCE=CCTTCCTTGTCTTGAATCTTGG
PRIMER_RIGHT_2_SEQUENCE=CGGGGACATCTACAAGTTGTTATT
PRIMER_LEFT_2=1442,22
PRIMER_RIGHT_2=1622,24
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=60.501
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=181
PRIMER_PAIR_PENALTY_3=2.6386
PRIMER_LEFT_3_PENALTY=0.137575
PRIMER_RIGHT_3_PENALTY=2.501065
PRIMER_LEFT_3_SEQUENCE=GATGGTCTTACCAGTGAGGGTC
PRIMER_RIGHT_3_SEQUENCE=CGGGGACATCTACAAGTTGTTATT
PRIMER_LEFT_3=1507,22
PRIMER_RIGHT_3=1622,24
PRIMER_LEFT_3_TM=59.862
PRIMER_RIGHT_3_TM=60.501
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=116
PRIMER_PAIR_PENALTY_4=2.7426
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=2.501065
PRIMER_LEFT_4_SEQUENCE=CTTCCTTGTCTTGAATCTTGGC
PRIMER_RIGHT_4_SEQUENCE=CGGGGACATCTACAAGTTGTTATT
PRIMER_LEFT_4=1443,22
PRIMER_RIGHT_4=1622,24
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=60.501
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=180
=
PRIMER_SEQUENCE_ID=SSR_02898 Ps_contig_mira-and-tgicl-ass_franssen_26039
SEQUENCE=CGGTAATTTTTAGGTTAAAGGGTAAGTCTTCCCTTCGATTCCAGAGTTCTTAGTCATCAAAGCTGCTGAAGATGTGCTTGTCTTGAAAAACACAGATGCCTTTTCCTTATCTAAACCAGTCATGCACATTTCAAGACCATAGACAGCTTCCGGTGAAGCTTCTAGTTTAGCACTGGCAGAGTAAATGTGCCAAATTTGTGACTTATCAGCATCACCCATCATGTAAGCTTGGCTACCAGAACCAAGTTTGCCAAAATAGCTGTCAAGAACATCAACTTCCTCAGAAAAGCTTCGATGAGGGAATGATTGAGCTCCCGGAAAAATGAAACTTCCACGAGTGTATCTAACCAATTTCACAGCAATGTTAAGACCACCAGCAAGCTTAAGAATTGCAGGGATAGACAGAAGCAACTTCGTAGTCCCACATGTTTTAATGATCAGTTTGTAAGCATACACAAACAAGCTTGATTCAGAAAGAACATACGAATCAACATAATCATTAGACAGTGAGTCAACAATAGTGCACTCTGCTGGGTTCAGAATTTCATCCAATTGGTCTTTAGACAATGCTCGGAGACCTAACCCAGCAGGATCAGAGAAAATACCATTCTCAAAAAACGTTATCTCAAGCCTCTTTTCGTATCCTTCAAAACCAATAGCTGAGTTTGTCATGGCCATCTTGACTCAAAGGAGAAAACAATTCGGTTGTTTGTAGAGAAATAGAACAGGAGAAGATGAAAAGCAACCAGGAGGAGGAGGAAAAGGAGAAGAGGAAACTAGAATAATTTTGCAGTATGAAAGATAAAAAACCTAAATCTATCAGAACCCAGAAAATCTCAGGAGGGTTTGCGGGTGCAGTTTGAGTAATCAGCAGATTTGAATTTTTCTACTCCACCGTTTGGTCGAACGTCTTCAATGATGTAATCGAGAGGAGCTTCGTATAAAGAGCATTTACTACTACTACTAGACTTCTTCTTACCACCTTTTGACTCCATTACTTCATTCACACCTTCTGTTGTTTAACAATTAGAGATCGATCTGTTTTTCGATTTCCCCTCTATGAATCAAGATCTCAGATTAAATGACAGCGATTGGAAAAAACTGAGGAACAGAAAAGAATGCAGAAAAATTGAAACAGAGCAACGGCAAGATTGAGAGAAAATTAGGTTTAGGAAATAGAAATTAGGATTGAAATTGGTTTCCCCGACGTACT
TARGET=748,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1953
PRIMER_LEFT_PENALTY=0.102139
PRIMER_RIGHT_PENALTY=0.093130
PRIMER_LEFT_SEQUENCE=CTCAAAGGAGAAAACAATTCGG
PRIMER_RIGHT_SEQUENCE=ACCAAACGGTGGAGTAGAAAAA
PRIMER_LEFT=683,22
PRIMER_RIGHT=902,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=59.907
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.2046
PRIMER_LEFT_1_PENALTY=0.102139
PRIMER_RIGHT_1_PENALTY=0.102490
PRIMER_LEFT_1_SEQUENCE=CTCAAAGGAGAAAACAATTCGG
PRIMER_RIGHT_1_SEQUENCE=CAAACCCTCCTGAGATTTTCTG
PRIMER_LEFT_1=683,22
PRIMER_RIGHT_1=848,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=166
PRIMER_PAIR_PENALTY_2=0.2460
PRIMER_LEFT_2_PENALTY=0.143555
PRIMER_RIGHT_2_PENALTY=0.102490
PRIMER_LEFT_2_SEQUENCE=TCTCAAGCCTCTTTTCGTATCC
PRIMER_RIGHT_2_SEQUENCE=CAAACCCTCCTGAGATTTTCTG
PRIMER_LEFT_2=623,22
PRIMER_RIGHT_2=848,22
PRIMER_LEFT_2_TM=59.856
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.3236
PRIMER_LEFT_3_PENALTY=0.221115
PRIMER_RIGHT_3_PENALTY=0.102490
PRIMER_LEFT_3_SEQUENCE=AGACAATGCTCGGAGACCTAAC
PRIMER_RIGHT_3_SEQUENCE=CAAACCCTCCTGAGATTTTCTG
PRIMER_LEFT_3=561,22
PRIMER_RIGHT_3=848,22
PRIMER_LEFT_3_TM=59.779
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.3347
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=0.093130
PRIMER_LEFT_4_SEQUENCE=AGGAGAAGATGAAAAGCAACCA
PRIMER_RIGHT_4_SEQUENCE=ACCAAACGGTGGAGTAGAAAAA
PRIMER_LEFT_4=726,22
PRIMER_RIGHT_4=902,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=59.907
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_02899 Ps_contig_mira-and-tgicl-ass_franssen_26039
SEQUENCE=CGGTAATTTTTAGGTTAAAGGGTAAGTCTTCCCTTCGATTCCAGAGTTCTTAGTCATCAAAGCTGCTGAAGATGTGCTTGTCTTGAAAAACACAGATGCCTTTTCCTTATCTAAACCAGTCATGCACATTTCAAGACCATAGACAGCTTCCGGTGAAGCTTCTAGTTTAGCACTGGCAGAGTAAATGTGCCAAATTTGTGACTTATCAGCATCACCCATCATGTAAGCTTGGCTACCAGAACCAAGTTTGCCAAAATAGCTGTCAAGAACATCAACTTCCTCAGAAAAGCTTCGATGAGGGAATGATTGAGCTCCCGGAAAAATGAAACTTCCACGAGTGTATCTAACCAATTTCACAGCAATGTTAAGACCACCAGCAAGCTTAAGAATTGCAGGGATAGACAGAAGCAACTTCGTAGTCCCACATGTTTTAATGATCAGTTTGTAAGCATACACAAACAAGCTTGATTCAGAAAGAACATACGAATCAACATAATCATTAGACAGTGAGTCAACAATAGTGCACTCTGCTGGGTTCAGAATTTCATCCAATTGGTCTTTAGACAATGCTCGGAGACCTAACCCAGCAGGATCAGAGAAAATACCATTCTCAAAAAACGTTATCTCAAGCCTCTTTTCGTATCCTTCAAAACCAATAGCTGAGTTTGTCATGGCCATCTTGACTCAAAGGAGAAAACAATTCGGTTGTTTGTAGAGAAATAGAACAGGAGAAGATGAAAAGCAACCAGGAGGAGGAGGAAAAGGAGAAGAGGAAACTAGAATAATTTTGCAGTATGAAAGATAAAAAACCTAAATCTATCAGAACCCAGAAAATCTCAGGAGGGTTTGCGGGTGCAGTTTGAGTAATCAGCAGATTTGAATTTTTCTACTCCACCGTTTGGTCGAACGTCTTCAATGATGTAATCGAGAGGAGCTTCGTATAAAGAGCATTTACTACTACTACTAGACTTCTTCTTACCACCTTTTGACTCCATTACTTCATTCACACCTTCTGTTGTTTAACAATTAGAGATCGATCTGTTTTTCGATTTCCCCTCTATGAATCAAGATCTCAGATTAAATGACAGCGATTGGAAAAAACTGAGGAACAGAAAAGAATGCAGAAAAATTGAAACAGAGCAACGGCAAGATTGAGAGAAAATTAGGTTTAGGAAATAGAAATTAGGATTGAAATTGGTTTCCCCGACGTACT
TARGET=953,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3655
PRIMER_LEFT_PENALTY=0.093130
PRIMER_RIGHT_PENALTY=0.272373
PRIMER_LEFT_SEQUENCE=TTTTTCTACTCCACCGTTTGGT
PRIMER_RIGHT_SEQUENCE=TTGATTCATAGAGGGGAAATCG
PRIMER_LEFT=881,22
PRIMER_RIGHT=1067,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.272
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.4846
PRIMER_LEFT_1_PENALTY=0.093130
PRIMER_RIGHT_1_PENALTY=0.391473
PRIMER_LEFT_1_SEQUENCE=TTTTTCTACTCCACCGTTTGGT
PRIMER_RIGHT_1_SEQUENCE=CCAATCGCTGTCATTTAATCTG
PRIMER_LEFT_1=881,22
PRIMER_RIGHT_1=1094,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=59.609
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=0.4890
PRIMER_LEFT_2_PENALTY=0.093130
PRIMER_RIGHT_2_PENALTY=0.395857
PRIMER_LEFT_2_SEQUENCE=TTTTTCTACTCCACCGTTTGGT
PRIMER_RIGHT_2_SEQUENCE=TAGAGGGGAAATCGAAAAACAG
PRIMER_LEFT_2=881,22
PRIMER_RIGHT_2=1059,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=59.604
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.5993
PRIMER_LEFT_3_PENALTY=0.093130
PRIMER_RIGHT_3_PENALTY=0.506150
PRIMER_LEFT_3_SEQUENCE=TTTTTCTACTCCACCGTTTGGT
PRIMER_RIGHT_3_SEQUENCE=CTGCATTCTTTTCTGTTCCTCA
PRIMER_LEFT_3=881,22
PRIMER_RIGHT_3=1123,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=59.494
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.5993
PRIMER_LEFT_4_PENALTY=0.093130
PRIMER_RIGHT_4_PENALTY=0.506150
PRIMER_LEFT_4_SEQUENCE=TTTTTCTACTCCACCGTTTGGT
PRIMER_RIGHT_4_SEQUENCE=TGCATTCTTTTCTGTTCCTCAG
PRIMER_LEFT_4=881,22
PRIMER_RIGHT_4=1122,22
PRIMER_LEFT_4_TM=59.907
PRIMER_RIGHT_4_TM=59.494
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_02900 Ps_contig_mira-and-tgicl-ass_franssen_26052
SEQUENCE=CAAANAGACCCTTCCTTTCTTCTCACTCATCTCTCTTCTTTTTTCCTTTTACTTCTATCATCACATTGAAGCTCTTCTTCCGACAACAGCAACAAAGTTCAAGAATCTTCCTCCAGGCCCCCCATTTCTTCCCATAATTGGAAACCTTCATCAACTCAAACAACCACTCCACCTCACTCTCCAAACCTTATCACAAAAACACGGCCAAATCTTTTCTCTCTGGTTCGGTTCTCGTCTCGTCGTCGTTGTTTCGTCGTTAAAAACAGCACAAGAATGTTTTACCAAACACGACATCGTTTTAGCGAACCGTCCTCATTTTTTATCCGGCAAATATATTGGCTACAACAACACCACAGTAGCACAGTCACCTTACGGTGACCACTGGCGCAATCTCCGCCGTATAATAACAACCGAGGTACTCTCATCTCACCGTTTGAACTCCTTCTTGGAAATACGAAGAGACGAGATCATGAGACTAATACAAAAGCTAGCACAAGTTTGTTACAGTGATTTCTCAGAAGTGGAACTTAGGCCAATGTTTTCCGAAATGACATTCAACACCATCATGAGAATGGTGTCTGGGAAAAGATACTACGGTGATGATTGCGACGTGAGCGATGTTGAGGAAGCTAGATTGTTCAGAGAGATAATCAAAGAGATTGTGAGTTTAGGAGGTGCTAACAACGTTGGTGATTTTCTAGGGTTTTTAAGGTGGTTCGATTTTGATGGTTTGGAGAAGAGGCTTAAGAGAATAAGCAAAAGAACAGATTCATTTTTACAAGGACTCATTGATGAACATAGATTTGGAAAAAGGAACACTAATACTATGATTGATCATCTCTTGAAACAGCAACAATCACAACCTGAATACTACACAGATGAAATCATCAAAGGACTTATTGTGGTTATGCTATTGGCAGGAACGGACACATCATCTTTAACCTTAGAATGGGCTATGTCTAATTTATTAAATAATCCAAAGATATTGAAGAAGGCAAAAGATGAATTGGACAATCACATGGGACAAGATCACTTGGTAGATGAACATGATATTTCAAAACTTCCTTATCTTCAAAGCATTATTCATGAGACTCTTCGATTACATCCGGCTGCTCCATTATTAGTGCCTCATTTGTCTTCCGAAGATTTTACAATTGAAGAATATAATATCCCACAAAACACAATTTTATTGGTCAATGCTTGGGTCATTCACAGAGATCCAAATTTGTGGACAAATCCTACATGTTTTAAGCCCGAAAGATTTGAGAAAGAAGGTGAGACAAATAAACTACTTTCATTTGGAATGGGAAGAAGGGTCTGCCCAGGAACAAATTTGGCACAACGCACAGTAAGTTTGGCTTTAGGCTTATTGATTCAGTGTTTTGAATGGACACGTATAGGTAAAGAAAAAATTGACATGACCGAAGGAAAAGGAATCACTGTGGGAAAAAAGATTTCCTTAAAAGTTAAGTGCAAATTACGTCATCCAGTGAAGATTAAATTTGCCTTCTAATTTATGAATGTTTGACATTTCTTAATAAGAGATACATGACGTAATCATCTTCAAATAATTTTTAAGTATGTAATTTCAAGATGATTTCAAGTAAATTTTTTAATTAATTAATATTTAGTTTTGGTAGG
TARGET=1613,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02901 Ps_contig_mira-and-tgicl-ass_franssen_26091
SEQUENCE=TTTNAAACTAACCCCTTTTCCCTTTTATTTGCTTTCAACACAAATCTAAGCAATGTTCACTTTCATTACATCAGTGTTTAAAGTATCATAAAACACATTTATTCATTCATTTTAATATACCATTGAAGCTTTCTGCTACATATTAAATTTCCAGAATTTTTTAAGTGGTAGATTTGGCTCTCTTTACTGGAGCTTGAAGAGAATTTAGATAAATTTCATCTATTTTGTCCAATCCATCAAACAGATAACTAAAAGGAGCCATTTTTATGTATTTTCCAGTACCAGGAACAACCTTCAGTTCATTATTTTCCCAAACAACTCTTCCTCCTGCTATAGTTACTTCAATTTTCCCCTTTCCTTTTCTTCCCTCATACACATTTGTATCAGATCTGGAGTGGTGAGATTTTGCAGTTATCTCAAAACTCGAGTTTGGATTCAGGATGATAATATCTGCATCGGAACCAGGAAGAATAGCTCCCTTCCTTGGATATATATTGAAGATTCTAGCACATTCAGTGCTCGTCACTCGGACGTAATCAGTGACCGATATTTGGCCAGATTCCACCATGGTGTCCCATACCAAGTGCATTCTTTCTTCAATACCATTGACGCCGTTTGGAATTTGGCGGAAGTCGTCGACTCCGAGAGCTTTCTGGGTGGAATTCCAGGCACAATGATCAGTTCCCACCAGCTGCAAAATCCCTGTTGCAAGGGCAGCTTGAAGGGCCTTATCATGTCCCTTACTCCTAATAGGAGGACTCATGACATACTTTGCCGCAGTCTTAAAGTCAGGATGCCAAAGCCAAGAATCGTCGAGGGCTAACCCAGAGACAATTGGCTCTCCGATTACCCTTTGTCCTGATTTCCGAGCCTTCGCAATTTCTTCCATCGCATCAATGCTCATGACATGAACCACATATAAAGGAGTGTTCACAAAATCTGCTAAGCGAATAGCACGAGAAGTTGCCTCTCCTTCCAACAACGCAGGCCTTGAAAGAGGATGACCTTCAGGGCCGGTTATTCCGAGTTCTATCATTTTCAGTTGCCCTGCTTCAACTGCATCTCCATTTTCTGCATGAACCATGGCTAAAGCACCGAGGGACTTGCATTTCTTAAACCCTTGAAGAAGAAGCTCATCATTGACCATAATGATTCCTTTGTATGCCATGAAAAACTTAAAAGAGTTGATGCCTTTCTCCTTGACCATGATCTCCATATCCCTTGAAACATCTTCGTTCCATTTGGTAATCGCGACATGGAAACCATAATCCATGCAAGACTTCTTTGCTTTATTTTCATAGGCTTCAAAACCAGCTGTTAAACTGCCATTAACCGGTATAATGAAGTCAATATGCATCGTTGTTCCACCAGCTAACGCTGCAGCCTGACCACTGAAGAAGTCATCTACCGATTCAGTGCCCAAAGCGCCCATCTCTAAGTGTGTGTGAGGATCAATGCCTCCTGGCATCACAAACTTGCCAGTGGCATCTATCACATACACATCATCTTCAACCACAATATTTGGCTGTACAGTAACAATGATTCCATTTTCTACATAAACATCAGCAACATGTTGGTGATGAGCATTGACCACAGTGCCACCTTTGATTAACAACTTGTTGGATGATGATTCACAGTATCCAATTCCATCTTCACAAAACTGGTTGTTAGAAACTTGTGAAAGTGAAATGATAAGAAAGAAGGAAAGAGAGAACAAGATATGAAGAAGTTGAAAACTGAAGTTCATCATCTTCATCATCATCACTGAACAACAGTAACCATAAACCGCAGACACAGCAACCTCGGCAACAGCTGTTTCTATATTTCCAGATTTTAGATAGTTTATCTATTATTATTTATTGAATTT
TARGET=1753,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0618
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.053515
PRIMER_LEFT_SEQUENCE=TCCATCTTCACAAAACTGGTTG
PRIMER_RIGHT_SEQUENCE=TGTCTGCGGTTTATGGTTACTG
PRIMER_LEFT=1645,22
PRIMER_RIGHT=1793,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.054
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=0.0618
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.053515
PRIMER_LEFT_1_SEQUENCE=TCCATCTTCACAAAACTGGTTG
PRIMER_RIGHT_1_SEQUENCE=CTGTGTCTGCGGTTTATGGTTA
PRIMER_LEFT_1=1645,22
PRIMER_RIGHT_1=1796,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.054
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.0822
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.073900
PRIMER_LEFT_2_SEQUENCE=TCCATCTTCACAAAACTGGTTG
PRIMER_RIGHT_2_SEQUENCE=TGTGTCTGCGGTTTATGGTTAC
PRIMER_LEFT_2=1645,22
PRIMER_RIGHT_2=1795,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=59.926
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=151
PRIMER_PAIR_PENALTY_3=0.4336
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.425301
PRIMER_LEFT_3_SEQUENCE=TCCATCTTCACAAAACTGGTTG
PRIMER_RIGHT_3_SEQUENCE=CTGCGGTTTATGGTTACTGTTG
PRIMER_LEFT_3=1645,22
PRIMER_RIGHT_3=1790,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.575
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.5140
PRIMER_LEFT_4_PENALTY=0.460467
PRIMER_RIGHT_4_PENALTY=0.053515
PRIMER_LEFT_4_SEQUENCE=CACAGTGCCACCTTTGATTAAC
PRIMER_RIGHT_4_SEQUENCE=TGTCTGCGGTTTATGGTTACTG
PRIMER_LEFT_4=1591,22
PRIMER_RIGHT_4=1793,22
PRIMER_LEFT_4_TM=59.540
PRIMER_RIGHT_4_TM=60.054
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_02902 Ps_contig_mira-and-tgicl-ass_franssen_26103
SEQUENCE=TGTTTTGGTAAGTTGAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTTCTTTTTTACTGTGTACACTT
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02903 Ps_contig_mira-and-tgicl-ass_franssen_26115
SEQUENCE=TCGGGGGTCTTAAAAATTCCACACAAATCGGGGGTACTAATATTATTCATTCATTCTTCTCACTATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCAACCCCCAAAACATGGAAACAACCCTCCACTCTATCTTTCATCTCTCTCAAACCAACCAAGTTCACACCAAGAACAACAAAAATTGCTGCAACAGAAGACAAAACCGATGCAGCAACCGATGTAGACAACAAAAGAAGCACCAGTTGGATTCACCCCACCTGAGCTAGACCCAAA
TARGET=45,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0399
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.039178
PRIMER_LEFT_SEQUENCE=CACACAAATCGGGGGTACTAAT
PRIMER_RIGHT_SEQUENCE=TTTTGTTGTCTACATCGGTTGC
PRIMER_LEFT=19,22
PRIMER_RIGHT=270,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.0406
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=CACACAAATCGGGGGTACTAAT
PRIMER_RIGHT_1_SEQUENCE=ATCCAACTGGTGCTTCTTTTGT
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=286,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.1278
PRIMER_LEFT_2_PENALTY=0.088662
PRIMER_RIGHT_2_PENALTY=0.039178
PRIMER_LEFT_2_SEQUENCE=GGGGTCTTAAAAATTCCACACA
PRIMER_RIGHT_2_SEQUENCE=TTTTGTTGTCTACATCGGTTGC
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=60.089
PRIMER_RIGHT_2_TM=60.039
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.1285
PRIMER_LEFT_3_PENALTY=0.088662
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=GGGGTCTTAAAAATTCCACACA
PRIMER_RIGHT_3_SEQUENCE=ATCCAACTGGTGCTTCTTTTGT
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=286,22
PRIMER_LEFT_3_TM=60.089
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.1368
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=CACACAAATCGGGGGTACTAAT
PRIMER_RIGHT_4_SEQUENCE=ATAGAGTGGAGGGTTGTTTCCA
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=171,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_02904 Ps_contig_mira-and-tgicl-ass_franssen_26115
SEQUENCE=TCGGGGGTCTTAAAAATTCCACACAAATCGGGGGTACTAATATTATTCATTCATTCTTCTCACTATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCAACCCCCAAAACATGGAAACAACCCTCCACTCTATCTTTCATCTCTCTCAAACCAACCAAGTTCACACCAAGAACAACAAAAATTGCTGCAACAGAAGACAAAACCGATGCAGCAACCGATGTAGACAACAAAAGAAGCACCAGTTGGATTCACCCCACCTGAGCTAGACCCAAA
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0399
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.039178
PRIMER_LEFT_SEQUENCE=CACACAAATCGGGGGTACTAAT
PRIMER_RIGHT_SEQUENCE=TTTTGTTGTCTACATCGGTTGC
PRIMER_LEFT=19,22
PRIMER_RIGHT=270,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.0406
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=CACACAAATCGGGGGTACTAAT
PRIMER_RIGHT_1_SEQUENCE=ATCCAACTGGTGCTTCTTTTGT
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=286,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.1278
PRIMER_LEFT_2_PENALTY=0.088662
PRIMER_RIGHT_2_PENALTY=0.039178
PRIMER_LEFT_2_SEQUENCE=GGGGTCTTAAAAATTCCACACA
PRIMER_RIGHT_2_SEQUENCE=TTTTGTTGTCTACATCGGTTGC
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=60.089
PRIMER_RIGHT_2_TM=60.039
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.1285
PRIMER_LEFT_3_PENALTY=0.088662
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=GGGGTCTTAAAAATTCCACACA
PRIMER_RIGHT_3_SEQUENCE=ATCCAACTGGTGCTTCTTTTGT
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=286,22
PRIMER_LEFT_3_TM=60.089
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.1368
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=CACACAAATCGGGGGTACTAAT
PRIMER_RIGHT_4_SEQUENCE=ATAGAGTGGAGGGTTGTTTCCA
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=171,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_02905 Ps_contig_mira-and-tgicl-ass_franssen_26133
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTTCTTTTTTTTACT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02906 Ps_contig_mira-and-tgicl-ass_franssen_26167
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTGTGTACNCTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02907 Ps_contig_mira-and-tgicl-ass_franssen_26236
SEQUENCE=GTCGGGGGTTGTTCTTGTCGTCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCGGACCTTTGCCGGAGAATGGTCTCGGCAAGCTCCGAAAACACCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGAATCAGAGGGTGGAAACGTGAGATCCTACGGGTGGGGATTCGTTAGAGGGTGCAGTGAAAGTGACACGTAGTATCATGATAGTGAAACCNGC
TARGET=117,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3621
PRIMER_LEFT_PENALTY=0.275745
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT=35,22
PRIMER_RIGHT=213,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.6994
PRIMER_LEFT_1_PENALTY=0.275745
PRIMER_RIGHT_1_PENALTY=0.423703
PRIMER_LEFT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_1_SEQUENCE=ATTCCTTGTCGGAGATAGATCG
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=152,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=59.576
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=0.8750
PRIMER_LEFT_2_PENALTY=0.275745
PRIMER_RIGHT_2_PENALTY=0.599276
PRIMER_LEFT_2_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_2_SEQUENCE=GTCACTTTCACTGCACCCTCTA
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=214,22
PRIMER_LEFT_2_TM=60.276
PRIMER_RIGHT_2_TM=59.401
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.9862
PRIMER_LEFT_3_PENALTY=0.275745
PRIMER_RIGHT_3_PENALTY=0.710449
PRIMER_LEFT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_3_SEQUENCE=ACTTTCACTGCACCCTCTAACG
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=60.710
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=1.0089
PRIMER_LEFT_4_PENALTY=0.922573
PRIMER_RIGHT_4_PENALTY=0.086339
PRIMER_LEFT_4_SEQUENCE=CTCATGAGCCTTCTCGATCACT
PRIMER_RIGHT_4_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=213,22
PRIMER_LEFT_4_TM=60.923
PRIMER_RIGHT_4_TM=59.914
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_02908 Ps_contig_mira-and-tgicl-ass_franssen_26290
SEQUENCE=CGGGGAGTAGATTCTGATGGTTCGCCAGTGGATTCAGTAACTGGAACTTCCTTTTTAATAGTTCCCACCTTAACATACTTCTCCATGAACTTGTATTCCCAGTCTTGTAATGCGTCGAGCTCAAATGGGCCAAGACCGGAGATGTCACCGGTCAGATCTTTATCTTCAAAAGACATCTTTGCTAAAGCTCTACTAGCATCCTTGCCAGCAAATAAAGCATAAGGACCGCCGGGTCCATAGAACATCCTGCTCTGGGAGACATCATAGATCTGACCCTTAATGGCCATGAGCAAGGGCTTATCGTGATCGTTACCATCGTAGGCCTTGAGCTCTTCTTCGGTGATTTCACCTAGCTGAACGGGGGTCGGAGAGGGGGAACCTCTGCTTGGATGTGGCGGGTTTGGTGGTGACGGTCGGAGGAGGAGGAGGAGCCAAAGAGACCGGAAACGACGTAGATAAGAGCAAATAGAATCGCCAATCCAGTGAAGRAAGTGGTCGGTGATAGACCGGTGAAGGAGAAGATGAGCTTCC
TARGET=416,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02909 Ps_contig_mira-and-tgicl-ass_franssen_26298
SEQUENCE=CTTAGTTAATGNGTTNTGTTCGGCCACCAAACTTATATAATTCAGAAATATTTAATTTGCATTACAACAATAATATAATAAGACCCATTCATCATGACATGACCGACACATTTATTCCATACAAAGGAAGCATCAAGAGAAGTAAACATGCATGCTTATATAGGTTACATGCATGTCATTACAAACATCACGCTAATAATAATTAAACACTCTCTTTATTTAATTACTATAATGCCTGAATGACTCATATAGCAACGTTTTCTCTTTTGATAAGATGACCATCAATATCTCTAGTGCATTGGTTAAGATAGTCCATCCATCCATTTGGAGGATGAATATCCTCATTGATTTTCTCATATTCAACAGTCCATTTAGCAACAGCAGTGCCATCAGCCTTATCAACTACTTCAAGAGTGAGCTTAAAGGCCTTATAGTGCTTATCTATTTCTCCACCAAACAGCTTCCATGTGTTCTTTTTGTTCTTTTCATCAACTTCTTCAATACTCTCATAACATGTGTGTACCTCACCACCTATGACATAAGTCCAGTGTTTAACTGTATCAGTGTCATGCCAATCTTCACCTTCATGAAGTTTGGTATGATGAACTCTTTCACAATGGACTTGCACTTCGTGAAGTTCTGATGCAAATAGTTTGTAAAACTTATCAGCGGGTGTTTTGATACCAAGTTCAGTGCTAAGCTTACCAGCTAGCACCATTTTTGAGAAGAAACTAAATATGTAATAATAGAAGAAAGAAAATAGATGCTATGATATCCCGCGACT
TARGET=312,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8044
PRIMER_LEFT_PENALTY=0.617089
PRIMER_RIGHT_PENALTY=0.187326
PRIMER_LEFT_SEQUENCE=CATGACCGACACATTTATTCCA
PRIMER_RIGHT_SEQUENCE=GCTGTTGCTAAATGGACTGTTG
PRIMER_LEFT=97,22
PRIMER_RIGHT=381,22
PRIMER_LEFT_TM=60.617
PRIMER_RIGHT_TM=59.813
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.8044
PRIMER_LEFT_1_PENALTY=0.617089
PRIMER_RIGHT_1_PENALTY=0.187326
PRIMER_LEFT_1_SEQUENCE=CATGACCGACACATTTATTCCA
PRIMER_RIGHT_1_SEQUENCE=TGCTGTTGCTAAATGGACTGTT
PRIMER_LEFT_1=97,22
PRIMER_RIGHT_1=382,22
PRIMER_LEFT_1_TM=60.617
PRIMER_RIGHT_1_TM=59.813
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.9335
PRIMER_LEFT_2_PENALTY=0.775205
PRIMER_RIGHT_2_PENALTY=0.158297
PRIMER_LEFT_2_SEQUENCE=CCATACAAAGGAAGCATCAAGA
PRIMER_RIGHT_2_SEQUENCE=AGTTGATAAGGCTGATGGCACT
PRIMER_LEFT_2=116,22
PRIMER_RIGHT_2=403,22
PRIMER_LEFT_2_TM=59.225
PRIMER_RIGHT_2_TM=60.158
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.9335
PRIMER_LEFT_3_PENALTY=0.775205
PRIMER_RIGHT_3_PENALTY=0.158297
PRIMER_LEFT_3_SEQUENCE=TCCATACAAAGGAAGCATCAAG
PRIMER_RIGHT_3_SEQUENCE=AGTTGATAAGGCTGATGGCACT
PRIMER_LEFT_3=115,22
PRIMER_RIGHT_3=403,22
PRIMER_LEFT_3_TM=59.225
PRIMER_RIGHT_3_TM=60.158
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=0.9625
PRIMER_LEFT_4_PENALTY=0.775205
PRIMER_RIGHT_4_PENALTY=0.187326
PRIMER_LEFT_4_SEQUENCE=TCCATACAAAGGAAGCATCAAG
PRIMER_RIGHT_4_SEQUENCE=GCTGTTGCTAAATGGACTGTTG
PRIMER_LEFT_4=115,22
PRIMER_RIGHT_4=381,22
PRIMER_LEFT_4_TM=59.225
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_02910 Ps_contig_mira-and-tgicl-ass_franssen_26301
SEQUENCE=AGCGGGNCCGGCCCAACCTAACCTAACTATACCTCTCTCTCTCTCTCTCTTCCGATTTTCGTTTTCTAGGTCATTTCAATTTCACGAAAATGAGAGAAATTCTTCATATCCAAGGTGGCCAATGCGGAAACCAGATCGGAGCCAAATTCTGGGAAGTTGTTTGCGCCGAACATGGAATCGACCCTACCGGAAGGTATGGTGGTGACACAGATCTTCAATTAGAGAGAATCAACGTCTATTACAACGAAGCTAGCTGTGGTCGTTATGTTCCTCGAGCGGTTCTTATGGATCTTGAACCGGGTACCATGGACAGTGTCCGGTCTGGACCGTACGGTCAGATTTTTAGACCGGATAACTTTGTGTTTGGTCAGAGTGGTGCTGGGAATAACTGGGCTAAGGGTCATTATACTGAAGGCGCTGAGCTTATTGATTCTGTTCTCGATGTTGTTCGGAAAGAAGCTGAAAACAGTGATTGCTTGCAGGGTTTTCAAGTATGTCATTCACTAGGTGGAGGAACTGGATCTGGAATGGGTACCCTTTTGATTTCAAAGATTAGGGAAGAATACCCTGATAGGATGATGCTTACTTTCTCCGTTTTTCCTTCTCCTAAGGTCTCTGACACAGTGGTCGAACCCTACAATGCTACTCTCTCGGTTCACCAGCTTGTTGAAAATGCTGATGAATGTATGGTTCTTGATAATGAAGCCTTGTATGATATTTGCTTCCGTACCCTTAAGCTCACAACCCCAAGCTTTGGTGATCTTAACCATTTGATATCTGCTACCATGTCTGGTGTCACATGTTGTCTGAGGTTCCCTGGTCAGCTTAACTCTGATCTTCGCAAGCTTGCTGTGAATCTGATTCCGTTCCCTCGTCTCCACTTTTTCATGTTGGGGTTTGCACCTTTGACTTCTAGAGGCTCTCAGCAGTACAGGGCTCTGAGTGTACCTGAAATCACACAGCAAATGTGGGATTCCAAGAACATGATGTGCGCTGCCGATCCTAGACATGGACGATACCTCACTGCATCCGCCATCTTCAGAGGCAAAATGAGCACAAAGGAAGTTGATGAACAAATGATGAATGTTCAGAACAAGAACTCATCCTACTTTGTGGAATGGATTCCAAACAATGTGAAATCAACTGTTTGTGATATTCCCCCAACAGGTTTGAAGATGGCTTCAACTTTCATTGGAAACTCAACCTCAATTCAGGAAATGTTCAGAAGGGTGAGTGAGCAATTCACTGCTATGTTTAGGAGGAAGGCTTTCTTGCATTGGTACACAGGTGAGGGAATGGACGAGATGGAATTCACCGAAGCCGAGAGCAACATGAACGATCTTGTATCAGAATACCAACAGTATCAAGATGCTACTGCTGAGGAGGATGAGTATGAAGAGGAAGAGGAAGATTATCATCAGGAACATGATGAAATGTGAGGACTATGATATGGATAGAATGATCTATTTGCGGTGGAATTTTGGATTGAATGTGGAACTTATCATGCCGCAACTTCCTGTTGCTGGTTCATTATCTATCTGTTTGTAATGCTATATATACTTCATTCCGGTTTATGTGAAATTAATTGATTATGCTTTTATTTCTGTTTGTTTGTGACATGTTAGGGAATGATATATTGATTGTTGAAACAGTTATTACTACTATATGCATCAACCTATTCTAGARTTAC
TARGET=33,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02911 Ps_contig_mira-and-tgicl-ass_franssen_26307
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTGTGTACACTTCT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02912 Ps_contig_mira-and-tgicl-ass_franssen_26318
SEQUENCE=GACAAAGAACACAAGACTGATGAAAATTCAAGCTGCTGGAGGATCAGTAGAAGTCCAGAAAATATCTTTAAGCGCAGGACGAAGCTGCTTGCTACAGAACTGATTATACTCCAAAGTATCACCCTTATCTTTCTTCACTTCCACCACCAGAAACGACGGCGTCACCGCGTAAATATCGGCGGCAATGGCCAACTTTCCTTTCCTCCCTCTTTCCTGTCCCTGAAGCCTCACCTTCGTATCACTACTCTTCACGTCGAACTTCACCGCCTTCGCCACTTGTTCCAGTTTCGATATCACTCTACTCGCCGTCCCCGCCGTTGCAAATCTCATCTCCTCCCTCTCTTCTCTCTTCTTCTCCTCAAACAAAGGAGACAAATCGAACCCTTCCGATAAAGATATTATATGAAAAGCATTCATCGTCGTCGGCGTCATCGACGATTTCTCCGCCTCCAGAAAACCAAATCCCTTCTCTTCTTCTTCCTCTTCTTTCTCTTTCTTCCTCCTCGCCACACTCTTCGGAACTGGTTTCTTGAACCAACAAGAATCCATTATCTGTGATATCGTAATTCTAGTATTCGGACTCGGATCAAGAAGCTTCGTGATAAGCCTTCTCGCATCAAGAGAAAACCACGGTGGACATTTGAAATCTCCTCGATAAATCTTCTTATACATAGCCACCAAATTATCATCCTGAAACGGTAAAAATCCGGCGAGAAGAACGTAGAGAATGACACCGCAAGACCAAATATCCGCCTTAGCACCGTCATAGCCTTTCTTCGCGATAACCTCCGGCGAAACATACGCCGGAGTACCACACGTGGTATGCAACAATCCGTCTTGTCTCAAATGCTCCGAGAAAGTACTGAGACCAAAATCAGATACCTTGAGATTACCTTCTTCGTCGAGGAGGAGATTCTCCGGCTTAAGATCACGGTGGTAAACACCGCGACTGTGACAGAAATCAACCGCGGAAATCAACTGCTGGAAATAGACTCTAGCAACGTCTTCCTTAAGACGACCTTTGACGATTTTGTTGAAAAGCTCACCGCCACGAACGAGTTCCATGGCGATGTAGATCTTCGATTTCGAAGCCATCACTTCATGGATTTGAACTATGTTCGAGTGTTTCACCATCTTCATCACTGAAATTTCTCTTTTGATTTGTTCCATCATTCCAACTTTTATCACTTTCTCTTTTCCAACTACTTTCATGGCTACGTTTTTTCCGGAATTCAAGTTTCGCGCGTGATAAACCTTCGCGAATGTTCCGTGACCCAGAACTCGACCCAGCTCGTATTTTCCATCGAGCAGAGCCGAGTGAGAACTCTCTTTTTCCTTCTCACACATGGCGGCTCGGCTTAACGGCTTGGTTATTATTTACAAAAATAATAGACTAGAAGAAGAAAGATGAAGGAGGGAGGGGTATTTTGGGAAAGAGAGAGGAAAAATGAGAAAAAAGATCAACCGTCTTCGACGTGTTTGCGGTGGCAATGTCTACCGCCGATGATCCATTTCGAAAGAACGATAAGTTCAGATGGAGAGAAACAACAACATTGGCCTCTCATCTCTCTGCATTCCTTCTTCTGCAGAGAAGAAGAAGAAAAACTATGATGAACAAAATAAGAATAAGAATTATTTATTTGCTGAGAAACTTGAATTGAAGAGTGTGTTAGGTTTTTGACAATCCCC
TARGET=1590,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0969
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.088662
PRIMER_LEFT_SEQUENCE=TGGAGAGAAACAACAACATTGG
PRIMER_RIGHT_SEQUENCE=GGGGATTGTCAAAAACCTAACA
PRIMER_LEFT=1535,22
PRIMER_RIGHT=1688,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.089
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.1094
PRIMER_LEFT_1_PENALTY=0.020747
PRIMER_RIGHT_1_PENALTY=0.088662
PRIMER_LEFT_1_SEQUENCE=CCTCTCATCTCTCTGCATTCCT
PRIMER_RIGHT_1_SEQUENCE=GGGGATTGTCAAAAACCTAACA
PRIMER_LEFT_1=1557,22
PRIMER_RIGHT_1=1688,22
PRIMER_LEFT_1_TM=59.979
PRIMER_RIGHT_1_TM=60.089
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=0.1285
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.088662
PRIMER_LEFT_2_SEQUENCE=AGAAACAACAACATTGGCCTCT
PRIMER_RIGHT_2_SEQUENCE=GGGGATTGTCAAAAACCTAACA
PRIMER_LEFT_2=1540,22
PRIMER_RIGHT_2=1688,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=60.089
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.1285
PRIMER_LEFT_3_PENALTY=0.039879
PRIMER_RIGHT_3_PENALTY=0.088662
PRIMER_LEFT_3_SEQUENCE=AGAGAAACAACAACATTGGCCT
PRIMER_RIGHT_3_SEQUENCE=GGGGATTGTCAAAAACCTAACA
PRIMER_LEFT_3=1538,22
PRIMER_RIGHT_3=1688,22
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=60.089
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.2277
PRIMER_LEFT_4_PENALTY=0.139083
PRIMER_RIGHT_4_PENALTY=0.088662
PRIMER_LEFT_4_SEQUENCE=ACAACATTGGCCTCTCATCTCT
PRIMER_RIGHT_4_SEQUENCE=GGGGATTGTCAAAAACCTAACA
PRIMER_LEFT_4=1547,22
PRIMER_RIGHT_4=1688,22
PRIMER_LEFT_4_TM=60.139
PRIMER_RIGHT_4_TM=60.089
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=142
=
PRIMER_SEQUENCE_ID=SSR_02913 Ps_contig_mira-and-tgicl-ass_franssen_26324
SEQUENCE=AGTACGACGGGTGACACAAAACCCTTGAAATCTCTGTTAACTCTTTCTCTGTGTTGTTTCATTCCTTTTGCATTTGATTTGAAGACGCCGTCGGCATAAACATGAAGGTGATCGCCGCATACTTGCTCGCCGTTCTCGGAGGCAACAACACCCCCTCCGCCAAAACCATCAAGGACATCCTTGGATCCGTTGGAGCAGAAGCAGAAGATGGCAACATTGAATTGTTTTTGTCTGAAATTAAGGGCAAAGATATAGCTGAGGTGATTGCTTCTGGTAAGGAAAAGTTGGCATCAGTGCCTTCTGGTGGTGGTGGTGGCGGTGTTGCTGTTGGTGCTGCACCTGCGAGTGGTGGTGCTGCTCCTGCAGCTGCCGAGGCAAAGAAAGAGGAGAAGGTTGAAGAGAAAGAGGAATCTGATGATGATATGGGATTCAGCCTTTTTGATTAAGTCTGTGGTTGCTGAGTTTATTTGAGTTTTGTTCCATAGTGTAGTGTGGTTTTATTTTATTTCAGTGATCAACGCCTAAGTGGACGAGTTTAGTTTAATATTGTAGTTTCTTAATGTTATGATGGAAGTTATATTTCTGCCATCACAGTGTAGCTTATTTCTTGTTTTACCTTTTCATCATACAGTTATTTCCTTTTTATCTTATTTTGAAGTTGAATACATTTATCTTATTTAAAATTTCCGTCCAGAATCATTTTTCAAGTAATATGATACTATACCAGTTGTGCTTTGTAACTGAGCATTTTTTGTACTGGTGTTAAGT
TARGET=302,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1059
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.105201
PRIMER_LEFT_SEQUENCE=GGTAAGGAAAAGTTGGCATCAG
PRIMER_RIGHT_SEQUENCE=ACCTTCTCCTCTTTCTTTGCCT
PRIMER_LEFT=272,22
PRIMER_RIGHT=393,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.895
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=122
PRIMER_PAIR_PENALTY_1=0.1059
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.105201
PRIMER_LEFT_1_SEQUENCE=CTGGTAAGGAAAAGTTGGCATC
PRIMER_RIGHT_1_SEQUENCE=ACCTTCTCCTCTTTCTTTGCCT
PRIMER_LEFT_1=270,22
PRIMER_RIGHT_1=393,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.895
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.1059
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.105201
PRIMER_LEFT_2_SEQUENCE=TCTGGTAAGGAAAAGTTGGCAT
PRIMER_RIGHT_2_SEQUENCE=ACCTTCTCCTCTTTCTTTGCCT
PRIMER_LEFT_2=269,22
PRIMER_RIGHT_2=393,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=59.895
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.1604
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.159680
PRIMER_LEFT_3_SEQUENCE=GGTAAGGAAAAGTTGGCATCAG
PRIMER_RIGHT_3_SEQUENCE=CTAAACTCGTCCACTTAGGCGT
PRIMER_LEFT_3=272,22
PRIMER_RIGHT_3=538,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=59.840
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=11.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.1604
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.159680
PRIMER_LEFT_4_SEQUENCE=TCTGGTAAGGAAAAGTTGGCAT
PRIMER_RIGHT_4_SEQUENCE=CTAAACTCGTCCACTTAGGCGT
PRIMER_LEFT_4=269,22
PRIMER_RIGHT_4=538,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.840
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_02914 Ps_contig_mira-and-tgicl-ass_franssen_26330
SEQUENCE=TGTTTGGTAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTGAAGCAAAAGATTTAACTCAAAGTTACAAAAAATCAGATACGAAAGGGTTCAGA
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9924
PRIMER_LEFT_PENALTY=0.371199
PRIMER_RIGHT_PENALTY=2.621188
PRIMER_LEFT_SEQUENCE=ATACGTTCTTCAATGGCGAAAC
PRIMER_RIGHT_SEQUENCE=CTGAACCCTTTCGTATCTGATT
PRIMER_LEFT=86,22
PRIMER_RIGHT=243,22
PRIMER_LEFT_TM=60.371
PRIMER_RIGHT_TM=57.379
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=3.0138
PRIMER_LEFT_1_PENALTY=0.392624
PRIMER_RIGHT_1_PENALTY=2.621188
PRIMER_LEFT_1_SEQUENCE=AGTTGAAAGGATTGGTGTTTGC
PRIMER_RIGHT_1_SEQUENCE=CTGAACCCTTTCGTATCTGATT
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=243,22
PRIMER_LEFT_1_TM=60.393
PRIMER_RIGHT_1_TM=57.379
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=3.0924
PRIMER_LEFT_2_PENALTY=0.471231
PRIMER_RIGHT_2_PENALTY=2.621188
PRIMER_LEFT_2_SEQUENCE=CATCAAGCAGGATCGGATAGTT
PRIMER_RIGHT_2_SEQUENCE=CTGAACCCTTTCGTATCTGATT
PRIMER_LEFT_2=31,22
PRIMER_RIGHT_2=243,22
PRIMER_LEFT_2_TM=60.471
PRIMER_RIGHT_2_TM=57.379
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=213
PRIMER_PAIR_PENALTY_3=3.5338
PRIMER_LEFT_3_PENALTY=0.912566
PRIMER_RIGHT_3_PENALTY=2.621188
PRIMER_LEFT_3_SEQUENCE=TTGAGGGATACGTTCTTCAATG
PRIMER_RIGHT_3_SEQUENCE=CTGAACCCTTTCGTATCTGATT
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=59.087
PRIMER_RIGHT_3_TM=57.379
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=3.6274
PRIMER_LEFT_4_PENALTY=1.006228
PRIMER_RIGHT_4_PENALTY=2.621188
PRIMER_LEFT_4_SEQUENCE=TGGTAAGTTGAAAGGATTGGTG
PRIMER_RIGHT_4_SEQUENCE=CTGAACCCTTTCGTATCTGATT
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=243,22
PRIMER_LEFT_4_TM=58.994
PRIMER_RIGHT_4_TM=57.379
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_02915 Ps_contig_mira-and-tgicl-ass_franssen_26350
SEQUENCE=ACCATTGATAAGAATACAATTATCAAAAGCATAACAATCTTTTCAATTTCATTGCAATATACACACACGATGGCCGCATCATCATCATCATCCATGGCTCTCCATTCCAACAATACTCTATCTATGGCTTTGCCGAATCAGCAAACCGTCGATTACCCGAGTTTCAAGCTTGTTATCGTCGGCGATGGTGGAACAGGAAAGACGAC
TARGET=77,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7565
PRIMER_LEFT_PENALTY=0.627348
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_SEQUENCE=CAAGCTTGAAACTCGGGTAATC
PRIMER_LEFT=51,22
PRIMER_RIGHT=171,22
PRIMER_LEFT_TM=59.373
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.7565
PRIMER_LEFT_1_PENALTY=0.627348
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_1_SEQUENCE=GATAACAAGCTTGAAACTCGGG
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=176,22
PRIMER_LEFT_1_TM=59.373
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.7896
PRIMER_LEFT_2_PENALTY=0.627348
PRIMER_RIGHT_2_PENALTY=0.162217
PRIMER_LEFT_2_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_2_SEQUENCE=AACAAGCTTGAAACTCGGGTAA
PRIMER_LEFT_2=51,22
PRIMER_RIGHT_2=173,22
PRIMER_LEFT_2_TM=59.373
PRIMER_RIGHT_2_TM=60.162
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=1.4277
PRIMER_LEFT_3_PENALTY=0.627348
PRIMER_RIGHT_3_PENALTY=0.800379
PRIMER_LEFT_3_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_3_SEQUENCE=ACAAGCTTGAAACTCGGGTAAT
PRIMER_LEFT_3=51,22
PRIMER_RIGHT_3=172,22
PRIMER_LEFT_3_TM=59.373
PRIMER_RIGHT_3_TM=59.200
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=1.4862
PRIMER_LEFT_4_PENALTY=0.627348
PRIMER_RIGHT_4_PENALTY=0.858861
PRIMER_LEFT_4_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_4_SEQUENCE=GTAATCGACGGTTTGCTGATTC
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=155,22
PRIMER_LEFT_4_TM=59.373
PRIMER_RIGHT_4_TM=60.859
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_02916 Ps_contig_mira-and-tgicl-ass_franssen_26367
SEQUENCE=TGTTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAAGGAAATTGAGTGTGGAACT
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1578
PRIMER_LEFT_PENALTY=0.149516
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=GTTTGGAAGTTGAAAGGATTGG
PRIMER_RIGHT_SEQUENCE=CCACACTCAATTTCCTTCACAA
PRIMER_LEFT=1,22
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=59.850
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.3795
PRIMER_LEFT_1_PENALTY=0.371199
PRIMER_RIGHT_1_PENALTY=0.008257
PRIMER_LEFT_1_SEQUENCE=ATACGTTCTTCAATGGCGAAAC
PRIMER_RIGHT_1_SEQUENCE=CCACACTCAATTTCCTTCACAA
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=227,22
PRIMER_LEFT_1_TM=60.371
PRIMER_RIGHT_1_TM=60.008
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.4795
PRIMER_LEFT_2_PENALTY=0.471231
PRIMER_RIGHT_2_PENALTY=0.008257
PRIMER_LEFT_2_SEQUENCE=CATCAAGCAGGATCGGATAGTT
PRIMER_RIGHT_2_SEQUENCE=CCACACTCAATTTCCTTCACAA
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=60.471
PRIMER_RIGHT_2_TM=60.008
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=0.6784
PRIMER_LEFT_3_PENALTY=0.149516
PRIMER_RIGHT_3_PENALTY=0.528861
PRIMER_LEFT_3_SEQUENCE=GTTTGGAAGTTGAAAGGATTGG
PRIMER_RIGHT_3_SEQUENCE=TCCACACTCAATTTCCTTCACA
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=228,22
PRIMER_LEFT_3_TM=59.850
PRIMER_RIGHT_3_TM=60.529
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=0.8912
PRIMER_LEFT_4_PENALTY=0.882980
PRIMER_RIGHT_4_PENALTY=0.008257
PRIMER_LEFT_4_SEQUENCE=ATCAAGCAGGATCGGATAGTTT
PRIMER_RIGHT_4_SEQUENCE=CCACACTCAATTTCCTTCACAA
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=59.117
PRIMER_RIGHT_4_TM=60.008
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=197
=
PRIMER_SEQUENCE_ID=SSR_02917 Ps_contig_mira-and-tgicl-ass_franssen_26368
SEQUENCE=GTAACCAACCAACCAACGACTTCGTCCCGAAYTTAACTAACCGTTGCGAGACAAAAGACTCTGGGAAGGACACATCCCAAACGACTCCCAACCATAGCCCATCTGGAGTGGATGACTTCAAGCTCACGGTTCTTGGAGAATGTCTCTGGGTCAGCAGAGAGTCCGGCAGTGTCCCAACCGTAGTCACCGGGGAACTCTCCAGTCAAGTAGGATGGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCTGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCAT
TARGET=3,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02918 Ps_contig_mira-and-tgicl-ass_franssen_26398
SEQUENCE=AACGNAGTACGTCGGGGACATCAATTAACTCCTAGATAACACACACACAACACAAAAAATGGGTAAAGATGTTGAGGTTCAAGAACAAGGTGCTGAATTCTCAGCTAAGGATTACCAAGATCCACCACCAGCACCACTTTTTGATTCAGCTGAGTTTAAAAAATGGTCTCTGTACAGAGCTGTTATAGCAGAGTTTGTAGCAACTCTTCTTTTTCTTTACATCACTGTTTTGACCATTATTGGTTACAAAAGACAATCTGATACCGATATTAAGGGTAACACTGAGTGTGATGGTGTTGGTCTTTTGGGTATTGCTTGGGCTTTTGGTGGTATGATCTTCATCCTTGTTTACTGCACCGCCGGTATCTCTGGAGGACACATAAATCCGGCAGTGACATTTGGATTATTTGTGGGACGCAAGGTGTCTTTAATAAGGGCATTACTATACATAATAGCACAATGTTTGGGTGCAATTTGTGGTGCTGGACTTGCTAAGGGGTTCCAAAGTTCATACTACAACAGGTATGGTGGAGGAGCTAACACTGTTTCTGATGGTTACAGTAAAGGCACTGCTTTGGGTGCTGAGATTATTGGTACCTTTGTGCTAGTCTACACTGTTTTCTCTGCTACTGATCCTAAAAGAAGTGCTAGGGATTCTCATGTTCCTGTATTGGCACCACTTCCCATTGGATTTGCTGTGTTCATGGTTCACTTGGCAACAATCCCCGTCACCGGAACCGGTATTAACCCTGCCAGAAGTTTCGGACCCGCAGTTATCTTCAACAACGACAAAGCTTGGGATGACCAGTGGATTTATTGGGTTGGACCATTTATTGGTGCTGCAGTGGCTGCAATCTACCACCAATACATTCTAAGAGGATCAGCAATTAAAGCTCTTGGATCCTTCAGGAGCAATGCTTAATGAATGCAACATGTTTGGCCTCAATTATTGTTATGAGTTGTTGATGTTGTTGTTGTTGTTGTCTACTGGTCCCTGTTGAAGTTTTTGTCCTCTTGCTTTCAATTCCACGTTCTGAATGGGCCTGTGTGTTTGGAATAATTGGAATGGATAAAGAAGCCAAAATGATAATGGGGTCCTCAAAACTGTAGATAAGCATGAATATGATTATGATTATGGTTATGGTTATGTATGTTATGTTTAAGGGTGTATTTGTATCCCTCCATTTTATCTTTTGTTATCTTTTTTGTTTTTGTATTTTATTCAAGGATTTCTCATGTGTTCTCTTTAATTTATGTCATAAGTTAGACTTTTTATCAGATTTTTCAACTTTAAGTTT
TARGET=39,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2695
PRIMER_LEFT_PENALTY=0.196811
PRIMER_RIGHT_PENALTY=0.072672
PRIMER_LEFT_SEQUENCE=CGGGGACATCAATTAACTCCTA
PRIMER_RIGHT_SEQUENCE=AGACCAACACCATCACACTCAG
PRIMER_LEFT=12,22
PRIMER_RIGHT=302,22
PRIMER_LEFT_TM=60.197
PRIMER_RIGHT_TM=60.073
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.4805
PRIMER_LEFT_1_PENALTY=0.196811
PRIMER_RIGHT_1_PENALTY=0.283676
PRIMER_LEFT_1_SEQUENCE=CGGGGACATCAATTAACTCCTA
PRIMER_RIGHT_1_SEQUENCE=TCAGCTGAATCAAAAAGTGGTG
PRIMER_LEFT_1=12,22
PRIMER_RIGHT_1=152,22
PRIMER_LEFT_1_TM=60.197
PRIMER_RIGHT_1_TM=60.284
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.4994
PRIMER_LEFT_2_PENALTY=0.196811
PRIMER_RIGHT_2_PENALTY=0.302618
PRIMER_LEFT_2_SEQUENCE=CGGGGACATCAATTAACTCCTA
PRIMER_RIGHT_2_SEQUENCE=AGCTGAATCAAAAAGTGGTGCT
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=150,22
PRIMER_LEFT_2_TM=60.197
PRIMER_RIGHT_2_TM=60.303
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=0.5093
PRIMER_LEFT_3_PENALTY=0.196811
PRIMER_RIGHT_3_PENALTY=0.312496
PRIMER_LEFT_3_SEQUENCE=CGGGGACATCAATTAACTCCTA
PRIMER_RIGHT_3_SEQUENCE=CAAAAGACCAACACCATCACAC
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=306,22
PRIMER_LEFT_3_TM=60.197
PRIMER_RIGHT_3_TM=60.312
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=0.6632
PRIMER_LEFT_4_PENALTY=0.196811
PRIMER_RIGHT_4_PENALTY=0.466342
PRIMER_LEFT_4_SEQUENCE=CGGGGACATCAATTAACTCCTA
PRIMER_RIGHT_4_SEQUENCE=AAGACCAACACCATCACACTCA
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=303,22
PRIMER_LEFT_4_TM=60.197
PRIMER_RIGHT_4_TM=60.466
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_02919 Ps_contig_mira-and-tgicl-ass_franssen_26398
SEQUENCE=AACGNAGTACGTCGGGGACATCAATTAACTCCTAGATAACACACACACAACACAAAAAATGGGTAAAGATGTTGAGGTTCAAGAACAAGGTGCTGAATTCTCAGCTAAGGATTACCAAGATCCACCACCAGCACCACTTTTTGATTCAGCTGAGTTTAAAAAATGGTCTCTGTACAGAGCTGTTATAGCAGAGTTTGTAGCAACTCTTCTTTTTCTTTACATCACTGTTTTGACCATTATTGGTTACAAAAGACAATCTGATACCGATATTAAGGGTAACACTGAGTGTGATGGTGTTGGTCTTTTGGGTATTGCTTGGGCTTTTGGTGGTATGATCTTCATCCTTGTTTACTGCACCGCCGGTATCTCTGGAGGACACATAAATCCGGCAGTGACATTTGGATTATTTGTGGGACGCAAGGTGTCTTTAATAAGGGCATTACTATACATAATAGCACAATGTTTGGGTGCAATTTGTGGTGCTGGACTTGCTAAGGGGTTCCAAAGTTCATACTACAACAGGTATGGTGGAGGAGCTAACACTGTTTCTGATGGTTACAGTAAAGGCACTGCTTTGGGTGCTGAGATTATTGGTACCTTTGTGCTAGTCTACACTGTTTTCTCTGCTACTGATCCTAAAAGAAGTGCTAGGGATTCTCATGTTCCTGTATTGGCACCACTTCCCATTGGATTTGCTGTGTTCATGGTTCACTTGGCAACAATCCCCGTCACCGGAACCGGTATTAACCCTGCCAGAAGTTTCGGACCCGCAGTTATCTTCAACAACGACAAAGCTTGGGATGACCAGTGGATTTATTGGGTTGGACCATTTATTGGTGCTGCAGTGGCTGCAATCTACCACCAATACATTCTAAGAGGATCAGCAATTAAAGCTCTTGGATCCTTCAGGAGCAATGCTTAATGAATGCAACATGTTTGGCCTCAATTATTGTTATGAGTTGTTGATGTTGTTGTTGTTGTTGTCTACTGGTCCCTGTTGAAGTTTTTGTCCTCTTGCTTTCAATTCCACGTTCTGAATGGGCCTGTGTGTTTGGAATAATTGGAATGGATAAAGAAGCCAAAATGATAATGGGGTCCTCAAAACTGTAGATAAGCATGAATATGATTATGATTATGGTTATGGTTATGTATGTTATGTTTAAGGGTGTATTTGTATCCCTCCATTTTATCTTTTGTTATCTTTTTTGTTTTTGTATTTTATTCAAGGATTTCTCATGTGTTCTCTTTAATTTATGTCATAAGTTAGACTTTTTATCAGATTTTTCAACTTTAAGTTT
TARGET=967,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0368
PRIMER_LEFT_PENALTY=0.024665
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=GCTGCAATCTACCACCAATACA
PRIMER_RIGHT_SEQUENCE=AGGACAAAAACTTCAACAGGGA
PRIMER_LEFT=847,22
PRIMER_RIGHT=1012,22
PRIMER_LEFT_TM=60.025
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.0368
PRIMER_LEFT_1_PENALTY=0.024665
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=GCTGCAATCTACCACCAATACA
PRIMER_RIGHT_1_SEQUENCE=GAGGACAAAAACTTCAACAGGG
PRIMER_LEFT_1=847,22
PRIMER_RIGHT_1=1013,22
PRIMER_LEFT_1_TM=60.025
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.1391
PRIMER_LEFT_2_PENALTY=0.024665
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=GCTGCAATCTACCACCAATACA
PRIMER_RIGHT_2_SEQUENCE=GGACAAAAACTTCAACAGGGAC
PRIMER_LEFT_2=847,22
PRIMER_RIGHT_2=1011,22
PRIMER_LEFT_2_TM=60.025
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.1391
PRIMER_LEFT_3_PENALTY=0.024665
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=GCTGCAATCTACCACCAATACA
PRIMER_RIGHT_3_SEQUENCE=GACAAAAACTTCAACAGGGACC
PRIMER_LEFT_3=847,22
PRIMER_RIGHT_3=1010,22
PRIMER_LEFT_3_TM=60.025
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.1412
PRIMER_LEFT_4_PENALTY=0.024665
PRIMER_RIGHT_4_PENALTY=0.116582
PRIMER_LEFT_4_SEQUENCE=GCTGCAATCTACCACCAATACA
PRIMER_RIGHT_4_SEQUENCE=GTGGAATTGAAAGCAAGAGGAC
PRIMER_LEFT_4=847,22
PRIMER_RIGHT_4=1029,22
PRIMER_LEFT_4_TM=60.025
PRIMER_RIGHT_4_TM=60.117
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_02920 Ps_contig_mira-and-tgicl-ass_franssen_26448
SEQUENCE=AAGCAGTGGTATCAAACGACAGAGTACGTCGGGGTACCATTGATAAGAATACAATTATCAAAAGCATAACAATCTTTTCAATTTCATTGCAATATACACACACGATGGCCGCATCATCATCATCATCCATGGCTCTCTCTTCTCCAACCTTGGCTGGCAAGCAACTCAAGCTGACCCCATCAAGCCAAGAATTGGGAGCTGCAAGGTTCACCATGAGGAAGTCTGCTACCAACAA
TARGET=112,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1614
PRIMER_LEFT_PENALTY=0.075027
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=GTCGGGGTACCATTGATAAGAA
PRIMER_RIGHT_SEQUENCE=TTGTTGGTAGCAGACTTCCTCA
PRIMER_LEFT=27,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=60.075
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.2835
PRIMER_LEFT_1_PENALTY=0.197140
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=GCAGTGGTATCAAACGACAGAG
PRIMER_RIGHT_1_SEQUENCE=TTGTTGGTAGCAGACTTCCTCA
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=234,22
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.2835
PRIMER_LEFT_2_PENALTY=0.197140
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=AGCAGTGGTATCAAACGACAGA
PRIMER_RIGHT_2_SEQUENCE=TTGTTGGTAGCAGACTTCCTCA
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=234,22
PRIMER_LEFT_2_TM=59.803
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=234
PRIMER_PAIR_PENALTY_3=0.7137
PRIMER_LEFT_3_PENALTY=0.627348
PRIMER_RIGHT_3_PENALTY=0.086339
PRIMER_LEFT_3_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_3_SEQUENCE=TTGTTGGTAGCAGACTTCCTCA
PRIMER_LEFT_3=86,22
PRIMER_RIGHT_3=234,22
PRIMER_LEFT_3_TM=59.373
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.8287
PRIMER_LEFT_4_PENALTY=0.075027
PRIMER_RIGHT_4_PENALTY=0.753664
PRIMER_LEFT_4_SEQUENCE=GTCGGGGTACCATTGATAAGAA
PRIMER_RIGHT_4_SEQUENCE=AGGTTGGAGAAGAGAGAGCCAT
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=149,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=60.754
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_02921 Ps_contig_mira-and-tgicl-ass_franssen_26531
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTGTGTACACTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02922 Ps_contig_mira-and-tgicl-ass_franssen_26602
SEQUENCE=CGGTCTGCCTCCCAAACTTACCCTAAAGCACCAAGATCAAATGTCCATCAATAACATTATAACTTATTAGAAATACACTTCTGTATCGCGACAACCACTATAAGGAATTTCACAATCTTCATACTCTAGTTTTAGAGTAGGTGTCAAACAAAGTCTTATTTCATATAAATAATCCAAATTATCAATCCGCTCACATCGAATTTGTGGCCTAAATAGAGTATGTGTCTGAATTGCATTGAGAATATTTTGTTTAGGATATGTTCCACCTGGTTTTGTACCATTCTTTTCAAGGATATCTAATATAATATGTCTATCATAAACGTTTAATGTTTCTTCAAAGTAAGAAATAAACTTGAACAATTGATCTGAACAAGTTCCGTGAGCATACCATTGATTAACCCATAATTGAATGTTAGCATCTTCTTGATGATCCTTTTTAAATAACGCCGGCCAATTTTCATTGAGTCTTGTCTTTATAGGAACAAAATCGTTCACATTTATGCCATCTTTGTTGCAATCTCGTATCTCAACACCTTCTTTACTTGGCCATAACCCATGGATAGTAAATTTTTGTGGCAATGGAGAAACACAATCATTATTTTTAAGCTTGCAGTATGTTGGTGGCCATGTCTGAGACAGTTTAAAATGGTCGAATAAAAGAGGTGGTGGTGGTGGTGGTGGTGGACGAGGAGGTGGTGGAGATCTTATCCTAGGTGGTGGTGGTGGAGAGANNCNCNTACAGGGGATAGGCAAGGCAC
TARGET=662,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3132
PRIMER_LEFT_PENALTY=0.064372
PRIMER_RIGHT_PENALTY=0.248820
PRIMER_LEFT_SEQUENCE=GGCCATAACCCATGGATAGTAA
PRIMER_RIGHT_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_LEFT=544,22
PRIMER_RIGHT=723,22
PRIMER_LEFT_TM=59.936
PRIMER_RIGHT_TM=59.751
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=0.3797
PRIMER_LEFT_1_PENALTY=0.130883
PRIMER_RIGHT_1_PENALTY=0.248820
PRIMER_LEFT_1_SEQUENCE=CCATCTTTGTTGCAATCTCGTA
PRIMER_RIGHT_1_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_LEFT_1=502,22
PRIMER_RIGHT_1=723,22
PRIMER_LEFT_1_TM=60.131
PRIMER_RIGHT_1_TM=59.751
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.3886
PRIMER_LEFT_2_PENALTY=0.139753
PRIMER_RIGHT_2_PENALTY=0.248820
PRIMER_LEFT_2_SEQUENCE=TGCAATCTCGTATCTCAACACC
PRIMER_RIGHT_2_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_LEFT_2=512,22
PRIMER_RIGHT_2=723,22
PRIMER_LEFT_2_TM=60.140
PRIMER_RIGHT_2_TM=59.751
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.5118
PRIMER_LEFT_3_PENALTY=0.263023
PRIMER_RIGHT_3_PENALTY=0.248820
PRIMER_LEFT_3_SEQUENCE=TCTTTACTTGGCCATAACCCAT
PRIMER_RIGHT_3_SEQUENCE=ACCACCACCACCTAGGATAAGA
PRIMER_LEFT_3=535,22
PRIMER_RIGHT_3=723,22
PRIMER_LEFT_3_TM=59.737
PRIMER_RIGHT_3_TM=59.751
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.7159
PRIMER_LEFT_4_PENALTY=0.064372
PRIMER_RIGHT_4_PENALTY=0.651564
PRIMER_LEFT_4_SEQUENCE=GGCCATAACCCATGGATAGTAA
PRIMER_RIGHT_4_SEQUENCE=CACCACCACCACCTAGGATAAG
PRIMER_LEFT_4=544,22
PRIMER_RIGHT_4=724,22
PRIMER_LEFT_4_TM=59.936
PRIMER_RIGHT_4_TM=60.652
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_02923 Ps_contig_mira-and-tgicl-ass_franssen_26602
SEQUENCE=CGGTCTGCCTCCCAAACTTACCCTAAAGCACCAAGATCAAATGTCCATCAATAACATTATAACTTATTAGAAATACACTTCTGTATCGCGACAACCACTATAAGGAATTTCACAATCTTCATACTCTAGTTTTAGAGTAGGTGTCAAACAAAGTCTTATTTCATATAAATAATCCAAATTATCAATCCGCTCACATCGAATTTGTGGCCTAAATAGAGTATGTGTCTGAATTGCATTGAGAATATTTTGTTTAGGATATGTTCCACCTGGTTTTGTACCATTCTTTTCAAGGATATCTAATATAATATGTCTATCATAAACGTTTAATGTTTCTTCAAAGTAAGAAATAAACTTGAACAATTGATCTGAACAAGTTCCGTGAGCATACCATTGATTAACCCATAATTGAATGTTAGCATCTTCTTGATGATCCTTTTTAAATAACGCCGGCCAATTTTCATTGAGTCTTGTCTTTATAGGAACAAAATCGTTCACATTTATGCCATCTTTGTTGCAATCTCGTATCTCAACACCTTCTTTACTTGGCCATAACCCATGGATAGTAAATTTTTGTGGCAATGGAGAAACACAATCATTATTTTTAAGCTTGCAGTATGTTGGTGGCCATGTCTGAGACAGTTTAAAATGGTCGAATAAAAGAGGTGGTGGTGGTGGTGGTGGTGGACGAGGAGGTGGTGGAGATCTTATCCTAGGTGGTGGTGGTGGAGAGANNCNCNTACAGGGGATAGGCAAGGCAC
TARGET=713,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1958
PRIMER_LEFT_PENALTY=0.064372
PRIMER_RIGHT_PENALTY=2.131423
PRIMER_LEFT_SEQUENCE=GGCCATAACCCATGGATAGTAA
PRIMER_RIGHT_SEQUENCE=CCTTGCCTATCCCCTGTANG
PRIMER_LEFT=544,22
PRIMER_RIGHT=754,20
PRIMER_LEFT_TM=59.936
PRIMER_RIGHT_TM=60.131
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.75
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.3250
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=2.2623
PRIMER_LEFT_1_PENALTY=0.130883
PRIMER_RIGHT_1_PENALTY=2.131423
PRIMER_LEFT_1_SEQUENCE=CCATCTTTGTTGCAATCTCGTA
PRIMER_RIGHT_1_SEQUENCE=CCTTGCCTATCCCCTGTANG
PRIMER_LEFT_1=502,22
PRIMER_RIGHT_1=754,20
PRIMER_LEFT_1_TM=60.131
PRIMER_RIGHT_1_TM=60.131
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.75
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=6.3250
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=2.2712
PRIMER_LEFT_2_PENALTY=0.139753
PRIMER_RIGHT_2_PENALTY=2.131423
PRIMER_LEFT_2_SEQUENCE=TGCAATCTCGTATCTCAACACC
PRIMER_RIGHT_2_SEQUENCE=CCTTGCCTATCCCCTGTANG
PRIMER_LEFT_2=512,22
PRIMER_RIGHT_2=754,20
PRIMER_LEFT_2_TM=60.140
PRIMER_RIGHT_2_TM=60.131
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.75
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.3250
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=243
PRIMER_PAIR_PENALTY_3=2.2754
PRIMER_LEFT_3_PENALTY=0.143968
PRIMER_RIGHT_3_PENALTY=2.131423
PRIMER_LEFT_3_SEQUENCE=AATAAAAGAGGTGGTGGTGGTG
PRIMER_RIGHT_3_SEQUENCE=CCTTGCCTATCCCCTGTANG
PRIMER_LEFT_3=652,22
PRIMER_RIGHT_3=754,20
PRIMER_LEFT_3_TM=60.144
PRIMER_RIGHT_3_TM=60.131
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.75
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.3250
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=2.3177
PRIMER_LEFT_4_PENALTY=0.186309
PRIMER_RIGHT_4_PENALTY=2.131423
PRIMER_LEFT_4_SEQUENCE=TAAGCTTGCAGTATGTTGGTGG
PRIMER_RIGHT_4_SEQUENCE=CCTTGCCTATCCCCTGTANG
PRIMER_LEFT_4=602,22
PRIMER_RIGHT_4=754,20
PRIMER_LEFT_4_TM=60.186
PRIMER_RIGHT_4_TM=60.131
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=57.895
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.75
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.3250
PRIMER_PAIR_4_COMPL_ANY=3.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_02924 Ps_contig_mira-and-tgicl-ass_franssen_26621
SEQUENCE=GATACTACGTGTTCACTTCTACTGNACCCTCCTAACCGAATCCCCACCCTACGGAGTCTCACGTTTCCACCCTCCTGATTCTGATCAAAACCTTAGTCGGAGATAGATCGGGAAAGGAAGGAAGGGTGTTTCGGAGCTTGCCGAGACCATTCTCCGGCAAAGGTCCGGCGACGGTGTCATCCCACAAGTGATCGAGAAGGCTCATGAGAATAGGGAAAGCGCAGACACCCCCGA
TARGET=114,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3668
PRIMER_LEFT_PENALTY=0.054514
PRIMER_RIGHT_PENALTY=0.312329
PRIMER_LEFT_SEQUENCE=TTAGTCGGAGATAGATCGGGAA
PRIMER_RIGHT_SEQUENCE=TTCCCTATTCTCATGAGCCTTC
PRIMER_LEFT=92,22
PRIMER_RIGHT=216,22
PRIMER_LEFT_TM=60.055
PRIMER_RIGHT_TM=59.688
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=125
PRIMER_PAIR_PENALTY_1=0.5407
PRIMER_LEFT_1_PENALTY=0.264946
PRIMER_RIGHT_1_PENALTY=0.275745
PRIMER_LEFT_1_SEQUENCE=CTACTGNACCCTCCTAACCGAA
PRIMER_RIGHT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=204,22
PRIMER_LEFT_1_TM=60.265
PRIMER_RIGHT_1_TM=60.276
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.50
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.5407
PRIMER_LEFT_2_PENALTY=0.264946
PRIMER_RIGHT_2_PENALTY=0.275745
PRIMER_LEFT_2_SEQUENCE=TTCTACTGNACCCTCCTAACCG
PRIMER_RIGHT_2_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=204,22
PRIMER_LEFT_2_TM=60.265
PRIMER_RIGHT_2_TM=60.276
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.50
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.5719
PRIMER_LEFT_3_PENALTY=0.296177
PRIMER_RIGHT_3_PENALTY=0.275745
PRIMER_LEFT_3_SEQUENCE=CTTAGTCGGAGATAGATCGGGA
PRIMER_RIGHT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_3=91,22
PRIMER_RIGHT_3=204,22
PRIMER_LEFT_3_TM=59.704
PRIMER_RIGHT_3_TM=60.276
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=0.5773
PRIMER_LEFT_4_PENALTY=0.264946
PRIMER_RIGHT_4_PENALTY=0.312329
PRIMER_LEFT_4_SEQUENCE=CTACTGNACCCTCCTAACCGAA
PRIMER_RIGHT_4_SEQUENCE=TTCCCTATTCTCATGAGCCTTC
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=216,22
PRIMER_LEFT_4_TM=60.265
PRIMER_RIGHT_4_TM=59.688
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.50
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_02925 Ps_contig_mira-and-tgicl-ass_franssen_26643
SEQUENCE=GTCGGGGAGGTATAAAGAAGGGTCGTTATCTCTTTGCGTTGTTCCTTTCTTCTTGACGGCGAGGCTTACCCTGTCCAAAATCACTGATTTCACAAAGATGAATCGCGAAAAATTGATGAAGATGGCCGGTTCAGTTCGAACTGGTGGAAAGGGTACCGTCAGAAGAAAGAAGAAGGCTGTCCACAAGACAACAACTACCGACGACAAAAGGCTTCAGAGTACTCTGAAGAGAATTGGGGTGAATGCCATCCCTCAAATTGAGGAGGTCAATATCTTTAAGGATGATGTGGTCATCCAGTTCTCAAACCCTAAAGTTCAAGCATCCATTGCAGCTAATACATGGGTTGTTAGTGGTGCTCCTCAAACCAAGAAGTTGCAAGACATACTTCCTAGCATTATCCACCAATTAGGGCCGGATAATTTGGAAAACCTGAAGAAGATAGCTGAGCAGTTCAATAAGCAGGTTCCTGAAGCAGGTGCTGGCACAGCCACAGCACAAGAAGAGAATGATGATGATGATGTTCCAGAGCTTGTCCCAGGAGAGACCTTTGAGACAGCTGCTGAGARGGACGGACCGAAAGGTAAGTTAAGGTTTAGGTTTGTTTATTTGTTTAGTTTTTTTACAAACAAAAGTAAAATT
TARGET=507,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02926 Ps_contig_mira-and-tgicl-ass_franssen_26645
SEQUENCE=AGAGTACTCGAACGGAATCCCGCTACAGGCTAGTTGCTTCAAATACCCTCACACACCGTGTCCTCGGGACTCAAACTAAACACAAACACAGTCTCTCTCTCTTTTCACCTATCGCGTCCCTCTTTCTCTCGGGCTCTCACTGCGTCAAAAACCACGAGAAGAGGAACAACATGCAGATCTTCGTCAAGACTTTAACCGGAAAAACCATCACTCTCGAGGTTGAGAGCAGCGACACCATCGACAACGTCAAGGCCAAGATCCAGGACAAGGAAGGTATCCCTCCTGACCAGCAGAGGTTGATTTTTGCTGGTAAGCAGCTGGAAGATGGTCGCACTCTTGCTGATTACAACATTCAAAAGGAATCGACGCTTCACTTGGTCCTGAGGCTCAGGGGAGGTACCATGATTAAAGTGAAAACTTTAACTGGTAAAGAAATTGAAATTGACATTGAGCCAACTGATACAATTGACCGTATCAAGGAACGTGTAGAAGAAAAGGAGGGAATTCCACCTGTGCAGCAGAGGCTCATATATGCTGGTAAGCAGCTTGCTGATGACAAAACAGCTAAAGAGTACAACATTGAGGGTGGTTCTGTACTTCACTTGGTGCTTGCATTGAGGGGTGGTACTTATTAAGGTAGAATGCACACCCGGATTCAAGAAATTAACTTGTTCAGGCTTGGTTATTTTCTAGTTCTTGGTAAATGTTTGAATTGCTTCTGAACCTAAAGACATGAAAGGAAGCTCTATACTGTCCGTCTCTGAGGACACGGTTTGTTCTTTTATCTGCTTGGTCTTTTTGTAATTTTGGGGTANTTTATTGGTGGTCGGTGTACCTNTTAAAGTAAAA
TARGET=92,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0712
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.038090
PRIMER_LEFT_SEQUENCE=CCTCGGGACTCAAACTAAACAC
PRIMER_RIGHT_SEQUENCE=ATGTTGTTCCTCTTCTCGTGGT
PRIMER_LEFT=61,22
PRIMER_RIGHT=171,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=111
PRIMER_PAIR_PENALTY_1=0.0754
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.038090
PRIMER_LEFT_1_SEQUENCE=TGCTTCAAATACCCTCACACAC
PRIMER_RIGHT_1_SEQUENCE=ATGTTGTTCCTCTTCTCGTGGT
PRIMER_LEFT_1=34,22
PRIMER_RIGHT_1=171,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=60.038
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=0.1513
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.118120
PRIMER_LEFT_2_SEQUENCE=CCTCGGGACTCAAACTAAACAC
PRIMER_RIGHT_2_SEQUENCE=TCTTGACGAAGATCTGCATGTT
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=188,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.882
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=128
PRIMER_PAIR_PENALTY_3=0.1555
PRIMER_LEFT_3_PENALTY=0.037339
PRIMER_RIGHT_3_PENALTY=0.118120
PRIMER_LEFT_3_SEQUENCE=TGCTTCAAATACCCTCACACAC
PRIMER_RIGHT_3_SEQUENCE=TCTTGACGAAGATCTGCATGTT
PRIMER_LEFT_3=34,22
PRIMER_RIGHT_3=188,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=59.882
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=0.1617
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.128591
PRIMER_LEFT_4_SEQUENCE=CCTCGGGACTCAAACTAAACAC
PRIMER_RIGHT_4_SEQUENCE=AGAGTGATGGTTTTTCCGGTTA
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=213,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=59.871
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_02927 Ps_contig_mira-and-tgicl-ass_franssen_26647
SEQUENCE=AAGCAGTGGTATCTAACGCAAGAAGTCGGGGGTTCACACAAATAAGAATAAGAACGAAGTTGCGAAACTAGGGTTTCCTTCTCCACACACACCAAATCGAATTCCATCCAGAGACCAAAACCAGTCGAATTTGAAGCGCAATCATGCCGAAGAACAAGGGAAAGGGAGGAAAGAATCGAAAGAGGGGTAAGAACGAAGCCGACGATGAGAAACGCGAGCTTGTTTTCAAAGAAGATGGCCAAGAATACGCTCAAGTTCTCCGCATGTTAGGTAACGGTAGATGCGAAGCTATGTGCATCGACGGTACAAAGCGTCTCTGTCACATCCGTGGGAAGATGCACAAAAAGGTCTGGATTGCCGCCGGCGATATCATACTCGTTGGTCTCCGTGATTATCAGGATGATAAGGCTGATGTTATTCTCAAATACATGCCGGATGAGGCTCGTCTTCTTAAGGCATATGGTGAGCTTCCTGATAATACTAGGCTTAATGAAGGTATTGGTGCTGGTCTTGATGAGGAAGATGATGGTGATGCTAATGATTATATCGAGTTTGAGGATGAGGATATTGATAAAATTTGAAAAAAAAAGTTTTTTTTGTTTGTTTGAAGTTAAAAAGTTGAATTCTAATTATTATTACTATCGTTATATGTGATGATGATGAACATGATGATATGTAATGTTTGGGGTTTTATGCTGTACTCTATTATGGGATATCACAGTGATGTTGATATCGTGAATTGAATTTCAATGCACAAGAATAAATTAATAGCTTAAGGTAAAANTTCTAG
TARGET=596,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0782
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=1.078158
PRIMER_LEFT_SEQUENCE=GTATTGGTGCTGGTCTTGATGA
PRIMER_RIGHT_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT=495,22
PRIMER_RIGHT=700,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=58.922
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=1.1864
PRIMER_LEFT_1_PENALTY=0.108206
PRIMER_RIGHT_1_PENALTY=1.078158
PRIMER_LEFT_1_SEQUENCE=ATGAAGGTATTGGTGCTGGTCT
PRIMER_RIGHT_1_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_1=489,22
PRIMER_RIGHT_1=700,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=58.922
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=1.1864
PRIMER_LEFT_2_PENALTY=0.108206
PRIMER_RIGHT_2_PENALTY=1.078158
PRIMER_LEFT_2_SEQUENCE=AGGTATTGGTGCTGGTCTTGAT
PRIMER_RIGHT_2_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_2=493,22
PRIMER_RIGHT_2=700,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=58.922
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=1.1939
PRIMER_LEFT_3_PENALTY=0.115769
PRIMER_RIGHT_3_PENALTY=1.078158
PRIMER_LEFT_3_SEQUENCE=TAAGGCATATGGTGAGCTTCCT
PRIMER_RIGHT_3_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_3=451,22
PRIMER_RIGHT_3=700,22
PRIMER_LEFT_3_TM=60.116
PRIMER_RIGHT_3_TM=58.922
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=1.2084
PRIMER_LEFT_4_PENALTY=0.130244
PRIMER_RIGHT_4_PENALTY=1.078158
PRIMER_LEFT_4_SEQUENCE=GGCTTAATGAAGGTATTGGTGC
PRIMER_RIGHT_4_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_4=483,22
PRIMER_RIGHT_4=700,22
PRIMER_LEFT_4_TM=59.870
PRIMER_RIGHT_4_TM=58.922
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_02928 Ps_contig_mira-and-tgicl-ass_franssen_26647
SEQUENCE=AAGCAGTGGTATCTAACGCAAGAAGTCGGGGGTTCACACAAATAAGAATAAGAACGAAGTTGCGAAACTAGGGTTTCCTTCTCCACACACACCAAATCGAATTCCATCCAGAGACCAAAACCAGTCGAATTTGAAGCGCAATCATGCCGAAGAACAAGGGAAAGGGAGGAAAGAATCGAAAGAGGGGTAAGAACGAAGCCGACGATGAGAAACGCGAGCTTGTTTTCAAAGAAGATGGCCAAGAATACGCTCAAGTTCTCCGCATGTTAGGTAACGGTAGATGCGAAGCTATGTGCATCGACGGTACAAAGCGTCTCTGTCACATCCGTGGGAAGATGCACAAAAAGGTCTGGATTGCCGCCGGCGATATCATACTCGTTGGTCTCCGTGATTATCAGGATGATAAGGCTGATGTTATTCTCAAATACATGCCGGATGAGGCTCGTCTTCTTAAGGCATATGGTGAGCTTCCTGATAATACTAGGCTTAATGAAGGTATTGGTGCTGGTCTTGATGAGGAAGATGATGGTGATGCTAATGATTATATCGAGTTTGAGGATGAGGATATTGATAAAATTTGAAAAAAAAAGTTTTTTTTGTTTGTTTGAAGTTAAAAAGTTGAATTCTAATTATTATTACTATCGTTATATGTGATGATGATGAACATGATGATATGTAATGTTTGGGGTTTTATGCTGTACTCTATTATGGGATATCACAGTGATGTTGATATCGTGAATTGAATTTCAATGCACAAGAATAAATTAATAGCTTAAGGTAAAANTTCTAG
TARGET=652,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0782
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=1.078158
PRIMER_LEFT_SEQUENCE=GTATTGGTGCTGGTCTTGATGA
PRIMER_RIGHT_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT=495,22
PRIMER_RIGHT=700,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=58.922
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=1.1864
PRIMER_LEFT_1_PENALTY=0.108206
PRIMER_RIGHT_1_PENALTY=1.078158
PRIMER_LEFT_1_SEQUENCE=ATGAAGGTATTGGTGCTGGTCT
PRIMER_RIGHT_1_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_1=489,22
PRIMER_RIGHT_1=700,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=58.922
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=1.1864
PRIMER_LEFT_2_PENALTY=0.108206
PRIMER_RIGHT_2_PENALTY=1.078158
PRIMER_LEFT_2_SEQUENCE=AGGTATTGGTGCTGGTCTTGAT
PRIMER_RIGHT_2_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_2=493,22
PRIMER_RIGHT_2=700,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=58.922
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=1.1939
PRIMER_LEFT_3_PENALTY=0.115769
PRIMER_RIGHT_3_PENALTY=1.078158
PRIMER_LEFT_3_SEQUENCE=TAAGGCATATGGTGAGCTTCCT
PRIMER_RIGHT_3_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_3=451,22
PRIMER_RIGHT_3=700,22
PRIMER_LEFT_3_TM=60.116
PRIMER_RIGHT_3_TM=58.922
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=1.2084
PRIMER_LEFT_4_PENALTY=0.130244
PRIMER_RIGHT_4_PENALTY=1.078158
PRIMER_LEFT_4_SEQUENCE=GGCTTAATGAAGGTATTGGTGC
PRIMER_RIGHT_4_SEQUENCE=GTACAGCATAAAACCCCAAACA
PRIMER_LEFT_4=483,22
PRIMER_RIGHT_4=700,22
PRIMER_LEFT_4_TM=59.870
PRIMER_RIGHT_4_TM=58.922
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_02929 Ps_contig_mira-and-tgicl-ass_franssen_26652
SEQUENCE=TCACGTGCTAAGGAATATGTTAGCTTGTCTTTAAGTTCAAATGATCCTGTCGGGTTTAACCTTGTTGATGTTTTGGCTTGGGGTTCTATAGGTCATATTGTTGCTTATTATATCTTGGCCACTTCTAGCAATGGTTATGATCCTAGTTTCTTTGGTAATTAAGATTACTATGTTGTTCGTTTATGTTGTTTTAATTTGTCTTTTCTTGCCCTTTTATGTTTATTAAGATGATGATGATGATGATGATAGATAGTTAATTAAGTTTTATTTTGTA
TARGET=227,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02930 Ps_contig_mira-and-tgicl-ass_franssen_26702
SEQUENCE=TGGTAGTCAACGACAGAGTCGGGGGGCAACAACAAAATCTACTTTGAGAAAACATGGGGTCTCAAATGGCCTCTATTACTCTCACTATTGCATTCACATTACTTTCTCTCACTTTGCCATCTCAAGCAAACAACTACATCTATTCATCTCCACCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCGTCAGAGAAGCCATACAAATATCCTTCTCCTCCACCACCAGTTTACAAGTACAAGTCACCACCTCCACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTCCTTACAAATATATATCTCCTCCTCCAACACCAATTTATAAATATAAGTCTCCTCCTCCACCTGTCTATGCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCACCACCGATTTACAAATATAAGTCTCCTCCTCCACCTGTCTATTCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCACCACCCGTTTACAAATATAAGTCTCCTCCTCCACCTGTCTATTCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCACCACCAGTTTACAAATATAAGTCTCCTCCTCCACCTGTCTATTCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGATTTATTCACCACCACCAGTTTACAAATATAAATCTCCTCCTCCACCTATTTACTCACCACCCCCACCACACTATGTCTACTCTTCACCTCCTCCACCGGTCTATTCCCCTCCTCCACCACATTACATCTACGCATGACCTCCTCCACCTTACCACTACTAAATAGTGGTTTACGGTAGATCCCTTGGATTTTAGGTATATATTGGAAATAAGCAGGAAGATGCATTGGCAATAATACAAGGAAGCAAAGTTTCAACGTAGTTTGTTTCAAGAAAAGCCACTCTTTTTATCTCATGTGTTTTGCTATTATCATTGTTAGTTTGTTTGGTTTTTGAACTTAGACAGTTCTAAGGCATGTGTGTAATGTATTTTGCAACCTACTCGTTTGAAGGGGTCACCAAAATTAAATAAAATATTACGTTCTACTATTCTATTTTTTCTAGC
TARGET=196,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0729
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=0.065960
PRIMER_LEFT_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_RIGHT_SEQUENCE=TGGAGGTGGTGACTTGTACTTG
PRIMER_LEFT=102,22
PRIMER_RIGHT=274,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=60.066
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.0814
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_RIGHT_1_SEQUENCE=ATATGGTGGAGGAGGTGAATTG
PRIMER_LEFT_1=102,22
PRIMER_RIGHT_1=304,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.1776
PRIMER_LEFT_2_PENALTY=0.006945
PRIMER_RIGHT_2_PENALTY=0.170703
PRIMER_LEFT_2_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_RIGHT_2_SEQUENCE=TGGATATTTGTATGGAGGAGGC
PRIMER_LEFT_2=102,22
PRIMER_RIGHT_2=343,22
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=60.171
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.2816
PRIMER_LEFT_3_PENALTY=0.006945
PRIMER_RIGHT_3_PENALTY=0.274705
PRIMER_LEFT_3_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_RIGHT_3_SEQUENCE=GGAGGAGGTGAATTGTATACCG
PRIMER_LEFT_3=102,22
PRIMER_RIGHT_3=297,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=59.725
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=0.2870
PRIMER_LEFT_4_PENALTY=0.220992
PRIMER_RIGHT_4_PENALTY=0.065960
PRIMER_LEFT_4_SEQUENCE=AGAAAACATGGGGTCTCAAATG
PRIMER_RIGHT_4_SEQUENCE=TGGAGGTGGTGACTTGTACTTG
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=60.221
PRIMER_RIGHT_4_TM=60.066
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_02931 Ps_contig_mira-and-tgicl-ass_franssen_26702
SEQUENCE=TGGTAGTCAACGACAGAGTCGGGGGGCAACAACAAAATCTACTTTGAGAAAACATGGGGTCTCAAATGGCCTCTATTACTCTCACTATTGCATTCACATTACTTTCTCTCACTTTGCCATCTCAAGCAAACAACTACATCTATTCATCTCCACCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCGTCAGAGAAGCCATACAAATATCCTTCTCCTCCACCACCAGTTTACAAGTACAAGTCACCACCTCCACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTCCTTACAAATATATATCTCCTCCTCCAACACCAATTTATAAATATAAGTCTCCTCCTCCACCTGTCTATGCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCACCACCGATTTACAAATATAAGTCTCCTCCTCCACCTGTCTATTCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCACCACCCGTTTACAAATATAAGTCTCCTCCTCCACCTGTCTATTCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCACCACCAGTTTACAAATATAAGTCTCCTCCTCCACCTGTCTATTCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGATTTATTCACCACCACCAGTTTACAAATATAAATCTCCTCCTCCACCTATTTACTCACCACCCCCACCACACTATGTCTACTCTTCACCTCCTCCACCGGTCTATTCCCCTCCTCCACCACATTACATCTACGCATGACCTCCTCCACCTTACCACTACTAAATAGTGGTTTACGGTAGATCCCTTGGATTTTAGGTATATATTGGAAATAAGCAGGAAGATGCATTGGCAATAATACAAGGAAGCAAAGTTTCAACGTAGTTTGTTTCAAGAAAAGCCACTCTTTTTATCTCATGTGTTTTGCTATTATCATTGTTAGTTTGTTTGGTTTTTGAACTTAGACAGTTCTAAGGCATGTGTGTAATGTATTTTGCAACCTACTCGTTTGAAGGGGTCACCAAAATTAAATAAAATATTACGTTCTACTATTCTATTTTTTCTAGC
TARGET=346,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1841
PRIMER_LEFT_PENALTY=0.170703
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=GCCTCCTCCATACAAATATCCA
PRIMER_RIGHT_SEQUENCE=AAATCGGTGGTGGTGAATAAAC
PRIMER_LEFT=322,22
PRIMER_RIGHT=515,22
PRIMER_LEFT_TM=60.171
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=0.4884
PRIMER_LEFT_1_PENALTY=0.482784
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_RIGHT_1_SEQUENCE=GTGAATAGACAGGTGGAGGAGG
PRIMER_LEFT_1=271,22
PRIMER_RIGHT_1=551,22
PRIMER_LEFT_1_TM=60.483
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.4884
PRIMER_LEFT_2_PENALTY=0.482784
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_RIGHT_2_SEQUENCE=GGTGAATAGACAGGTGGAGGAG
PRIMER_LEFT_2=271,22
PRIMER_RIGHT_2=552,22
PRIMER_LEFT_2_TM=60.483
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.4962
PRIMER_LEFT_3_PENALTY=0.482784
PRIMER_RIGHT_3_PENALTY=0.013401
PRIMER_LEFT_3_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_RIGHT_3_SEQUENCE=AAATCGGTGGTGGTGAATAAAC
PRIMER_LEFT_3=271,22
PRIMER_RIGHT_3=515,22
PRIMER_LEFT_3_TM=60.483
PRIMER_RIGHT_3_TM=59.987
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.4962
PRIMER_LEFT_4_PENALTY=0.482784
PRIMER_RIGHT_4_PENALTY=0.013401
PRIMER_LEFT_4_SEQUENCE=CCTCCACCGGTATACAATTCAC
PRIMER_RIGHT_4_SEQUENCE=AAATCGGTGGTGGTGAATAAAC
PRIMER_LEFT_4=269,22
PRIMER_RIGHT_4=515,22
PRIMER_LEFT_4_TM=60.483
PRIMER_RIGHT_4_TM=59.987
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_02932 Ps_contig_mira-and-tgicl-ass_franssen_26702
SEQUENCE=TGGTAGTCAACGACAGAGTCGGGGGGCAACAACAAAATCTACTTTGAGAAAACATGGGGTCTCAAATGGCCTCTATTACTCTCACTATTGCATTCACATTACTTTCTCTCACTTTGCCATCTCAAGCAAACAACTACATCTATTCATCTCCACCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCGTCAGAGAAGCCATACAAATATCCTTCTCCTCCACCACCAGTTTACAAGTACAAGTCACCACCTCCACCGGTATACAATTCACCTCCTCCACCATATAAGTATCCATCTCCACCGCCTCCTCCATACAAATATCCATCACCACCACCACCTGTTTACAAGTACAACTCACCTCCTCCTCCTTACAAATATATATCTCCTCCTCCAACACCAATTTATAAATATAAGTCTCCTCCTCCACCTGTCTATGCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCACCACCGATTTACAAATATAAGTCTCCTCCTCCACCTGTCTATTCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCACCACCCGTTTACAAATATAAGTCTCCTCCTCCACCTGTCTATTCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGGTTTATTCACCACCACCAGTTTACAAATATAAGTCTCCTCCTCCACCTGTCTATTCACCACCAATTTACAAATATAAATCTCCTCCTCCACCGATTTATTCACCACCACCAGTTTACAAATATAAATCTCCTCCTCCACCTATTTACTCACCACCCCCACCACACTATGTCTACTCTTCACCTCCTCCACCGGTCTATTCCCCTCCTCCACCACATTACATCTACGCATGACCTCCTCCACCTTACCACTACTAAATAGTGGTTTACGGTAGATCCCTTGGATTTTAGGTATATATTGGAAATAAGCAGGAAGATGCATTGGCAATAATACAAGGAAGCAAAGTTTCAACGTAGTTTGTTTCAAGAAAAGCCACTCTTTTTATCTCATGTGTTTTGCTATTATCATTGTTAGTTTGTTTGGTTTTTGAACTTAGACAGTTCTAAGGCATGTGTGTAATGTATTTTGCAACCTACTCGTTTGAAGGGGTCACCAAAATTAAATAAAATATTACGTTCTACTATTCTATTTTTTCTAGC
TARGET=378,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1841
PRIMER_LEFT_PENALTY=0.170703
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=GCCTCCTCCATACAAATATCCA
PRIMER_RIGHT_SEQUENCE=AAATCGGTGGTGGTGAATAAAC
PRIMER_LEFT=322,22
PRIMER_RIGHT=515,22
PRIMER_LEFT_TM=60.171
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=0.4884
PRIMER_LEFT_1_PENALTY=0.482784
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_RIGHT_1_SEQUENCE=GTGAATAGACAGGTGGAGGAGG
PRIMER_LEFT_1=271,22
PRIMER_RIGHT_1=551,22
PRIMER_LEFT_1_TM=60.483
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.4884
PRIMER_LEFT_2_PENALTY=0.482784
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_RIGHT_2_SEQUENCE=GGTGAATAGACAGGTGGAGGAG
PRIMER_LEFT_2=271,22
PRIMER_RIGHT_2=552,22
PRIMER_LEFT_2_TM=60.483
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.4962
PRIMER_LEFT_3_PENALTY=0.482784
PRIMER_RIGHT_3_PENALTY=0.013401
PRIMER_LEFT_3_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_RIGHT_3_SEQUENCE=AAATCGGTGGTGGTGAATAAAC
PRIMER_LEFT_3=271,22
PRIMER_RIGHT_3=515,22
PRIMER_LEFT_3_TM=60.483
PRIMER_RIGHT_3_TM=59.987
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.4962
PRIMER_LEFT_4_PENALTY=0.482784
PRIMER_RIGHT_4_PENALTY=0.013401
PRIMER_LEFT_4_SEQUENCE=CCTCCACCGGTATACAATTCAC
PRIMER_RIGHT_4_SEQUENCE=AAATCGGTGGTGGTGAATAAAC
PRIMER_LEFT_4=269,22
PRIMER_RIGHT_4=515,22
PRIMER_LEFT_4_TM=60.483
PRIMER_RIGHT_4_TM=59.987
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_02933 Ps_contig_mira-and-tgicl-ass_franssen_26722
SEQUENCE=CATGGAGAACATAGCCAGCCGACCATTCTTGAGTTCCTTGACCTTCAACTCTGCAAATGAATCAGGGTCATCAGCAAGGCCAAGAGGGTCAAAAGCACCACCAGGGTAAAGTGGGTCTAGTCCATAAAAACCACCACCAAATGTCATAAAGTTCTTCTGGTCCTTGAGACCAGCATCACTTGGTACATCTCTTGCACTTGTTGCAGCAACAACAACAAGAGCAAGAACCAGACAAAAAACATACACCACTTACG
TARGET=207,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02934 Ps_contig_mira-and-tgicl-ass_franssen_26731
SEQUENCE=ACTTGTCTTTCTCTTGCTGGGTCCGTCCNTCCTCTTACTTTCTCTTCCACCCACTCTCCCTCCTCTCNTCACCCTTCCCTACGTTTCTTCTCTCTTCTCTCTCTCTCTCTCTCCCTCCTTTCTTCCTCATGGCTGAGGATAAGGAGATGTCTACTTCTGTTATCAACGGGAATGATTCTCTCACTGGTCAAATAATATCTACAACAATTGGAGGCAAAAACGGTGAACCCAAACAGACTATTAGTTACATGGCAGAACGTGTTGTAGGAACTGGATCATTTGGAATTGTTTTCCAGGCGAAATGCTTGGAAACCGGAGAGGCAGTGGCCATTAAAAAGGTTTTACAAGACCGGAGATATAAGAACCGTGAACTACAGTTGATGCGTGTGATGGATCATCCAAATGTGGTCACACTGAAGCATTGTTTCTTTTCAACTACAAGTACAGATGAACTTTTTCTTAATTTGGTGATGGAATATGTTCCAGAATCCATGTATCGGGTCTTAAAGCACTATAACAACGCAAATCAAAGAATACCAATCATCTATGTAAAACTTTACATGTACCAGATCTTTAGGGGGTTGGCTTATATCCATACTGTTCCTGGAGTTTGCCACAGGGATTTAAAGCCTCAAAACATACTGGTTGATCCTCTTTCACATCAAGTTAAGCTATGTGATTTCGGAAGTGCGAAAATGCTGGTCAAGGGCGAAGCTAATATATCATACATATGTTCACGATTCTATCGAGCTCCAGAGCTTATATTTGGTGCCACAGAGTATACTACTTCAATTGATATTTGGTCAGGTGGCTGTGTCCTTGCTGAACTTCTTTTGGGCCAGCCATTATTCCCTGGTGAAAATGCGGTGGACCAGCTCGTACATATTATAAAGGTGCTTGGAACACCCACCCGGGAGGAAGTTCGTTGCATGAATCCCAATTACAATGACTTTAGGTTTCCGCAAATAAAAGCACACCCATGGCACAAGATCTTCCACAAAAAGATGCCTCCAGAAGCTATCGATCTTGCTTCACGGCTTTTGCAGTACTCCCCAAGTCTCCGTTGCACTGCGCTTGAAGCTTGTGCACATCCTTTCTTTGATGATCTTCGAGAACCCAATGCCCGTCTGCCTAATGGTCGCCCATTTCCTCCTCTTTTCAACTTTAAACAGGAATTATCAGGAGCATCCCCAGAGCTTGTTAACAAGTTGATACCTGACCACATAAAGCGGCAAATAGGGCTACAATTTGTTCAATCAACCGGATCATGATGATAATCATAGGAGGCAAAGTGTATACTAGTTGATACATTAAAACAAAAGCGGCACCATTATTTTTTGTCTAAGTTATCATGTTCTGTGGCCTGGCCAGTGTAGTTTCACGCTGAACCCGGACTGGATGGCTGCAGTTAATCCCTCAGTATATGTGTATACCTTTTGACTATATCTTGCAAAAGAATAGCTTTTTTTCATTGAATGTTTATTGTAAAATGTCTACATGATGTCATGGTATAGTTGGCCTAAATTGGTCAGAAAATGCTTTTGTGAGAGGAAAACCAAACAAACCCTTATAGCTGGATGGTGGGTCCCATAAAAACAAAGGTGGGTCTTTGGAGTTTTGATGAGATATTGTGATGGGCTATGTTGGTTGTTGGGTTTGGTTTTCTTAACTCTTATCATGTTAATATGTGCTTGATTTCATTGCAATGTTGCAGACCAGTTACTTTCCTTGTCGAATGTAATATCATATACCTTTCTTAACTTTGCTTCATGTCCTTTTGATAAC
TARGET=96,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0371
PRIMER_LEFT_PENALTY=0.001908
PRIMER_RIGHT_PENALTY=0.035219
PRIMER_LEFT_SEQUENCE=ACCCTTCCCTACGTTTCTTCTC
PRIMER_RIGHT_SEQUENCE=ACAACACGTTCTGCCATGTAAC
PRIMER_LEFT=70,22
PRIMER_RIGHT=264,22
PRIMER_LEFT_TM=60.002
PRIMER_RIGHT_TM=59.965
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.0976
PRIMER_LEFT_1_PENALTY=0.001908
PRIMER_RIGHT_1_PENALTY=0.095684
PRIMER_LEFT_1_SEQUENCE=ACCCTTCCCTACGTTTCTTCTC
PRIMER_RIGHT_1_SEQUENCE=CAACACGTTCTGCCATGTAACT
PRIMER_LEFT_1=70,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=60.002
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=194
PRIMER_PAIR_PENALTY_2=0.1037
PRIMER_LEFT_2_PENALTY=0.068468
PRIMER_RIGHT_2_PENALTY=0.035219
PRIMER_LEFT_2_SEQUENCE=CCGTCCNTCCTCTTACTTTCTC
PRIMER_RIGHT_2_SEQUENCE=ACAACACGTTCTGCCATGTAAC
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=264,22
PRIMER_LEFT_2_TM=59.932
PRIMER_RIGHT_2_TM=59.965
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=243
PRIMER_PAIR_PENALTY_3=0.1037
PRIMER_LEFT_3_PENALTY=0.068468
PRIMER_RIGHT_3_PENALTY=0.035219
PRIMER_LEFT_3_SEQUENCE=TCCGTCCNTCCTCTTACTTTCT
PRIMER_RIGHT_3_SEQUENCE=ACAACACGTTCTGCCATGTAAC
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=264,22
PRIMER_LEFT_3_TM=59.932
PRIMER_RIGHT_3_TM=59.965
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.1287
PRIMER_LEFT_4_PENALTY=0.068468
PRIMER_RIGHT_4_PENALTY=0.060196
PRIMER_LEFT_4_SEQUENCE=TCCGTCCNTCCTCTTACTTTCT
PRIMER_RIGHT_4_SEQUENCE=ATCTCCTTATCCTCAGCCATGA
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=147,22
PRIMER_LEFT_4_TM=59.932
PRIMER_RIGHT_4_TM=60.060
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=127
=
PRIMER_SEQUENCE_ID=SSR_02935 Ps_contig_mira-and-tgicl-ass_franssen_26792
SEQUENCE=CGGGACATCTACAAGTTGTTATTTAGTTTTAGAGCAAAGAGATTCATACATACATATCACGCAACACCGTTTCAAGTATAGATGCAAATCTTCGTTAAGACCCTCACTGGTAAGACCATCACTCTCGAAGTTGAGAGTTCAGATACCATAGACAATGTTAAGGCCAAGATTCAAGACAAGGAAGGAATCCCTCCAGACCAGCAGCGTCTCATTTTTGCTGGCAAGCAACTAGAGGATGGGCGTACCCTTGCTGATTACAATATACAGAAGGAATCAACTCTCCATTTGGTGCTCCGTCTCCGTGGYGGGATGCAAATCTTCGTGAAGACTCTCACTGGGAAAGACCATCCACCCCTTTGGAGGGTTGGAGA
TARGET=45,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02936 Ps_contig_mira-and-tgicl-ass_franssen_26811
SEQUENCE=TGGACGTNACNTCACAGTAAACGAAGCTCGGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCCGGGATCGTGGGTATGGAGGAAGTGGTGGAGGAGGCGGTTATTCTCGTGGAGGAGGCGGTGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGGTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTGCACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAATTAGAAAAAATAGTGAGATATTAGTGGTTACAGATTTTGATAATGAANNCGTTTCTTACT
TARGET=87,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0798
PRIMER_LEFT_PENALTY=1.048692
PRIMER_RIGHT_PENALTY=0.031122
PRIMER_LEFT_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_SEQUENCE=CACTACTCCCTCCAGTTTCCAC
PRIMER_LEFT=10,21
PRIMER_RIGHT=284,22
PRIMER_LEFT_TM=59.951
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=1.0798
PRIMER_LEFT_1_PENALTY=1.048692
PRIMER_RIGHT_1_PENALTY=0.031122
PRIMER_LEFT_1_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_1_SEQUENCE=ACACTACTCCCTCCAGTTTCCA
PRIMER_LEFT_1=10,21
PRIMER_RIGHT_1=285,22
PRIMER_LEFT_1_TM=59.951
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=1.6961
PRIMER_LEFT_2_PENALTY=1.048692
PRIMER_RIGHT_2_PENALTY=0.647458
PRIMER_LEFT_2_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_2_SEQUENCE=ATAACCACCACCACCATATCCT
PRIMER_LEFT_2=10,21
PRIMER_RIGHT_2=189,22
PRIMER_LEFT_2_TM=59.951
PRIMER_RIGHT_2_TM=59.353
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=1.7430
PRIMER_LEFT_3_PENALTY=1.048692
PRIMER_RIGHT_3_PENALTY=0.694262
PRIMER_LEFT_3_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_3_SEQUENCE=ATATCCTCCTCCTCCACTACCG
PRIMER_LEFT_3=10,21
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=59.951
PRIMER_RIGHT_3_TM=60.694
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=1.7453
PRIMER_LEFT_4_PENALTY=1.714220
PRIMER_RIGHT_4_PENALTY=0.031122
PRIMER_LEFT_4_SEQUENCE=NTCACAGTAAACGAAGCTCGGA
PRIMER_RIGHT_4_SEQUENCE=CACTACTCCCTCCAGTTTCCAC
PRIMER_LEFT_4=10,22
PRIMER_RIGHT_4=284,22
PRIMER_LEFT_4_TM=61.714
PRIMER_RIGHT_4_TM=60.031
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_02937 Ps_contig_mira-and-tgicl-ass_franssen_26811
SEQUENCE=TGGACGTNACNTCACAGTAAACGAAGCTCGGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCCGGGATCGTGGGTATGGAGGAAGTGGTGGAGGAGGCGGTTATTCTCGTGGAGGAGGCGGTGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGGTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTGCACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAATTAGAAAAAATAGTGAGATATTAGTGGTTACAGATTTTGATAATGAANNCGTTTCTTACT
TARGET=140,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0798
PRIMER_LEFT_PENALTY=1.048692
PRIMER_RIGHT_PENALTY=0.031122
PRIMER_LEFT_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_SEQUENCE=CACTACTCCCTCCAGTTTCCAC
PRIMER_LEFT=10,21
PRIMER_RIGHT=284,22
PRIMER_LEFT_TM=59.951
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=1.0798
PRIMER_LEFT_1_PENALTY=1.048692
PRIMER_RIGHT_1_PENALTY=0.031122
PRIMER_LEFT_1_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_1_SEQUENCE=ACACTACTCCCTCCAGTTTCCA
PRIMER_LEFT_1=10,21
PRIMER_RIGHT_1=285,22
PRIMER_LEFT_1_TM=59.951
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=1.6961
PRIMER_LEFT_2_PENALTY=1.048692
PRIMER_RIGHT_2_PENALTY=0.647458
PRIMER_LEFT_2_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_2_SEQUENCE=ATAACCACCACCACCATATCCT
PRIMER_LEFT_2=10,21
PRIMER_RIGHT_2=189,22
PRIMER_LEFT_2_TM=59.951
PRIMER_RIGHT_2_TM=59.353
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=1.7430
PRIMER_LEFT_3_PENALTY=1.048692
PRIMER_RIGHT_3_PENALTY=0.694262
PRIMER_LEFT_3_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_3_SEQUENCE=ATATCCTCCTCCTCCACTACCG
PRIMER_LEFT_3=10,21
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=59.951
PRIMER_RIGHT_3_TM=60.694
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=1.7453
PRIMER_LEFT_4_PENALTY=1.714220
PRIMER_RIGHT_4_PENALTY=0.031122
PRIMER_LEFT_4_SEQUENCE=NTCACAGTAAACGAAGCTCGGA
PRIMER_RIGHT_4_SEQUENCE=CACTACTCCCTCCAGTTTCCAC
PRIMER_LEFT_4=10,22
PRIMER_RIGHT_4=284,22
PRIMER_LEFT_4_TM=61.714
PRIMER_RIGHT_4_TM=60.031
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_02938 Ps_contig_mira-and-tgicl-ass_franssen_26811
SEQUENCE=TGGACGTNACNTCACAGTAAACGAAGCTCGGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCCGGGATCGTGGGTATGGAGGAAGTGGTGGAGGAGGCGGTTATTCTCGTGGAGGAGGCGGTGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGGTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTGCACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAATTAGAAAAAATAGTGAGATATTAGTGGTTACAGATTTTGATAATGAANNCGTTTCTTACT
TARGET=161,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0798
PRIMER_LEFT_PENALTY=1.048692
PRIMER_RIGHT_PENALTY=0.031122
PRIMER_LEFT_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_SEQUENCE=CACTACTCCCTCCAGTTTCCAC
PRIMER_LEFT=10,21
PRIMER_RIGHT=284,22
PRIMER_LEFT_TM=59.951
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=1.0798
PRIMER_LEFT_1_PENALTY=1.048692
PRIMER_RIGHT_1_PENALTY=0.031122
PRIMER_LEFT_1_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_1_SEQUENCE=ACACTACTCCCTCCAGTTTCCA
PRIMER_LEFT_1=10,21
PRIMER_RIGHT_1=285,22
PRIMER_LEFT_1_TM=59.951
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=1.2514
PRIMER_LEFT_2_PENALTY=1.220325
PRIMER_RIGHT_2_PENALTY=0.031122
PRIMER_LEFT_2_SEQUENCE=TGGAGGAGGAGGATACGGTAGT
PRIMER_RIGHT_2_SEQUENCE=ACACTACTCCCTCCAGTTTCCA
PRIMER_LEFT_2=138,22
PRIMER_RIGHT_2=285,22
PRIMER_LEFT_2_TM=61.220
PRIMER_RIGHT_2_TM=60.031
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.1000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=1.3063
PRIMER_LEFT_3_PENALTY=1.220325
PRIMER_RIGHT_3_PENALTY=0.085951
PRIMER_LEFT_3_SEQUENCE=GGAGGAGGAGGATACGGTAGTG
PRIMER_RIGHT_3_SEQUENCE=AGATCCAGTGCAGAACAACAGA
PRIMER_LEFT_3=139,22
PRIMER_RIGHT_3=410,22
PRIMER_LEFT_3_TM=61.220
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=59.091
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=272
PRIMER_PAIR_PENALTY_4=1.3063
PRIMER_LEFT_4_PENALTY=1.220325
PRIMER_RIGHT_4_PENALTY=0.085951
PRIMER_LEFT_4_SEQUENCE=GTGGAGGAGGAGGATACGGTAG
PRIMER_RIGHT_4_SEQUENCE=AGATCCAGTGCAGAACAACAGA
PRIMER_LEFT_4=137,22
PRIMER_RIGHT_4=410,22
PRIMER_LEFT_4_TM=61.220
PRIMER_RIGHT_4_TM=59.914
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_02939 Ps_contig_mira-and-tgicl-ass_franssen_26811
SEQUENCE=TGGACGTNACNTCACAGTAAACGAAGCTCGGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCCGGGATCGTGGGTATGGAGGAAGTGGTGGAGGAGGCGGTTATTCTCGTGGAGGAGGCGGTGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGGTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTGCACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAATTAGAAAAAATAGTGAGATATTAGTGGTTACAGATTTTGATAATGAANNCGTTTCTTACT
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0798
PRIMER_LEFT_PENALTY=1.048692
PRIMER_RIGHT_PENALTY=0.031122
PRIMER_LEFT_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_SEQUENCE=CACTACTCCCTCCAGTTTCCAC
PRIMER_LEFT=10,21
PRIMER_RIGHT=284,22
PRIMER_LEFT_TM=59.951
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=1.0798
PRIMER_LEFT_1_PENALTY=1.048692
PRIMER_RIGHT_1_PENALTY=0.031122
PRIMER_LEFT_1_SEQUENCE=NTCACAGTAAACGAAGCTCGG
PRIMER_RIGHT_1_SEQUENCE=ACACTACTCCCTCCAGTTTCCA
PRIMER_LEFT_1=10,21
PRIMER_RIGHT_1=285,22
PRIMER_LEFT_1_TM=59.951
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=1.2514
PRIMER_LEFT_2_PENALTY=1.220325
PRIMER_RIGHT_2_PENALTY=0.031122
PRIMER_LEFT_2_SEQUENCE=ATACGGTAGTGGAGGAGGAGGA
PRIMER_RIGHT_2_SEQUENCE=ACACTACTCCCTCCAGTTTCCA
PRIMER_LEFT_2=150,22
PRIMER_RIGHT_2=285,22
PRIMER_LEFT_2_TM=61.220
PRIMER_RIGHT_2_TM=60.031
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=1.2514
PRIMER_LEFT_3_PENALTY=1.220325
PRIMER_RIGHT_3_PENALTY=0.031122
PRIMER_LEFT_3_SEQUENCE=TACGGTAGTGGAGGAGGAGGAT
PRIMER_RIGHT_3_SEQUENCE=CACTACTCCCTCCAGTTTCCAC
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=284,22
PRIMER_LEFT_3_TM=61.220
PRIMER_RIGHT_3_TM=60.031
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=134
PRIMER_PAIR_PENALTY_4=1.2514
PRIMER_LEFT_4_PENALTY=1.220325
PRIMER_RIGHT_4_PENALTY=0.031122
PRIMER_LEFT_4_SEQUENCE=TGGAGGAGGAGGATACGGTAGT
PRIMER_RIGHT_4_SEQUENCE=ACACTACTCCCTCCAGTTTCCA
PRIMER_LEFT_4=138,22
PRIMER_RIGHT_4=285,22
PRIMER_LEFT_4_TM=61.220
PRIMER_RIGHT_4_TM=60.031
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_02940 Ps_contig_mira-and-tgicl-ass_franssen_26822
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATTAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTTACTTTGTGTACACTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02941 Ps_contig_mira-and-tgicl-ass_franssen_26918
SEQUENCE=GTAAGAAAAGGATTAAGAACATAAGCCTGCTCAAGATGAGGCGATTGCCCACGAGTTTGGTCACAAGGTGACTTTGTATGAAGCACCGTCGGAAACCAAAGTTGAAGAGGAAGGTGAAAAGAAACACACCAGTCTCTTGGAGAAACTTCACCGATCTGACAGCTCATCAAGCTCCTCAAGTGAAGAGGAAGGAGAAGATGGTGAGAAAAGAAGAAGAAGAAGGAAAAGAAGAAAGAAGAAAACTACGTNGCCGTNAGAGTAGGNAGGTAAAGGTNANGTANNAGRNGACGACACGACGGAGAGGACGAACGGACGACGACGAGGAGAG
TARGET=208,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02942 Ps_contig_mira-and-tgicl-ass_franssen_26920
SEQUENCE=CNGACTACCTGCGAAATCGAACATTCAAACGTGGAAGTTTCTCTCATCTCTAGTCAGAAGAACCTTCCCTGAATCGCAGCCACTTATTATCAACTTAATCCAAACCCCAAACGCTCTTTCTTCCCAAACACACTCCAAACAAAAACTAGGGTTTCCAATTTTCAATTTCCCCCAAATGATGTTCTTACACCTCTAGGGTTTCCACAAGGTCTCGATTCTCAATCAGTATATGCGATCATCGTTAATCATACGAACGAGAAGAAGAAGAAGAAGATGTATAGCAATTTCAAGGAGCAAGCAATAGAATACGTGAAACAAGCTGTACAGGAAGATAATGCGGGAAACTACGCAAAAGCGTTTCCTCTATACATGAACGCGTTGGAGTATTTCAAAACGCATCTCAAGTACGAAAAGAATCCCAAAATTAAAGAAGCTATTACTCAGAAATTTACTGAGTATCTTCGTCGGGCTGAGGAGATCCGAACTGTTCTTGATGATGGCGGGCCGGGACCTGCTTCTAATGGAGATGCTGCTGTTGCTACGAAGCCCAAAACGAAGCCGAAGAATGGCGAGGGAGATGGAGAGGATCCTGAGCAGTCGAAGCTTAGGGCTGGCTTGAATTCTGCGATTGTGAGGGAGAAACCGAATGTGAAGTGGAATGATGTTGCTGGGTTGGAGAGTGCTAAGCAATCGTTGCAGGAAGCTGTTATCTTGCCTGTGAAGTTTCCTCAGTTTTTCACAGGTAAAAGACGTCCTTGGAGAGCATTTTTGCTGTATGGACCACCTGGAACAGGTAAATCATACTTGGCTAAGGCTGTTGCAACTGAAGCCGATTCTACATTTTTCAGTGTTTCTTCATCAGACCTCGTTTCAAAGTGGATGGGTGAAAGTGAAAAGCTGGTTTCAAATCTTTTTGAAATGGCTCGCGAAAGTGCACCTTCCATCATATTTGTTGATGAAATAGATTCTCTATGTGGTACGCGTGGAGAAGGCAATGAGAGTGAAGCTTCAAGACGAATTAAAACTGAACTTCTGGTGCAGATGCAGGGGGTTGGACACAATGATCAAAAAGTTCTCGTTCTTGCAGCAACAAATACACCATATGCTCTAGACCAGGCAATAAGGCGGCGTTTTGATAAGCGTATATACATTCCACTACCAGATTTAAAGGCTCGCCAACACATGTTCAAGGTGCATCTAGGAGACACTCCTCATAATTTGACTGAAAGTGATTTTGAACACTTGGCTCGTAAGTCAGAGGGGTTTTCAGGTTCAGATGTATCTGTCTGCGTGAAGGATGTTTTGTTCGAACCTGTTCGTAAAACCCAAGATGCCATGTTTTTCTTTAAGAGTCCAGAAGGTATGTGGATACCATGTGGACAAAAGCAACAAAATGCAGTACAAATTACAATGCAAGATCTTGCCACACAGGGACTTGCTTCAAAGATCCTTCCTCCACCTATATCAAGAACCGATTTTGACAAAGTACTCGCTAGACAAAGGCCTACAGTAAGCAAATCTGACCTCGATGTTCACGAGAGATTCACCAAGGAGTTCGGAGAGGAAGGTTAATAATGCTAATGGGTTTTATTTCAAAGATCCACACATGTTTAAACATATTGTTATAATTTGTACAAGTTGTAAGTTACGGAGCTATTGGATCAAATGAAATGCATTCCTTTTTTTTATTATCATGGTGGATGTGAATTTCCCTCTTGTATGGAAGTGTATGCTGATTTTACTTCTAAATTGTGTATAAAAACAGAATCTGAAATAATCAGAAAAATAATTTGCATGTGATGTTGGTTGCTTACGTTGGCCTGT
TARGET=257,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0646
PRIMER_LEFT_PENALTY=0.054514
PRIMER_RIGHT_PENALTY=0.010077
PRIMER_LEFT_SEQUENCE=TTCCCCCAAATGATGTTCTTAC
PRIMER_RIGHT_SEQUENCE=CGTAGTTTCCCGCATTATCTTC
PRIMER_LEFT=166,22
PRIMER_RIGHT=348,22
PRIMER_LEFT_TM=60.055
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.2064
PRIMER_LEFT_1_PENALTY=0.054514
PRIMER_RIGHT_1_PENALTY=0.151911
PRIMER_LEFT_1_SEQUENCE=TTCCCCCAAATGATGTTCTTAC
PRIMER_RIGHT_1_SEQUENCE=TCCAACGCGTTCATGTATAGAG
PRIMER_LEFT_1=166,22
PRIMER_RIGHT_1=382,22
PRIMER_LEFT_1_TM=60.055
PRIMER_RIGHT_1_TM=60.152
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.2153
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.010077
PRIMER_LEFT_2_SEQUENCE=GCTCTTTCTTCCCAAACACACT
PRIMER_RIGHT_2_SEQUENCE=CGTAGTTTCCCGCATTATCTTC
PRIMER_LEFT_2=112,22
PRIMER_RIGHT_2=348,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=59.990
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=0.2824
PRIMER_LEFT_3_PENALTY=0.272373
PRIMER_RIGHT_3_PENALTY=0.010077
PRIMER_LEFT_3_SEQUENCE=ACCTCTAGGGTTTCCACAAGGT
PRIMER_RIGHT_3_SEQUENCE=CGTAGTTTCCCGCATTATCTTC
PRIMER_LEFT_3=188,22
PRIMER_RIGHT_3=348,22
PRIMER_LEFT_3_TM=60.272
PRIMER_RIGHT_3_TM=59.990
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=0.3195
PRIMER_LEFT_4_PENALTY=0.309418
PRIMER_RIGHT_4_PENALTY=0.010077
PRIMER_LEFT_4_SEQUENCE=TCAGAAGAACCTTCCCTGAATC
PRIMER_RIGHT_4_SEQUENCE=CGTAGTTTCCCGCATTATCTTC
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=348,22
PRIMER_LEFT_4_TM=59.691
PRIMER_RIGHT_4_TM=59.990
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_02943 Ps_contig_mira-and-tgicl-ass_franssen_26927
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTTACTGTG
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02944 Ps_contig_mira-and-tgicl-ass_franssen_26931
SEQUENCE=TTGTCGGGGACACCAAAACCTTTAACCCATCCTTTCTCTTCTTAGCCAAACACAATCATGGCATCAATGACCATACTACCCTTCTTTCTCTTCTTCTTCTTTATCACTTTCTCATTAGCATCAAACATACCAAATGGTCGTAATAACGAGGAGGTTATGACCATGTACGAGGAGTGGTTGGTAAAACACCAAAAAGTGTATAACGGGTTAGGAGAGAAAGACCAAAGATTTCAAATTTTTAAGGATAATTTAAAATTCATTGATGAACACAATGCTCAAAATTACACTTATATTGTTGGGTTGAACAAGTTTGCAGATATAACTAATGAAGAATATCGTGGCATGTATTTGGGAACAAGGAGTGATGCCAAACGTAGGATCATGAAAAGTAAGATCACCGGTCACCGGTATGCTTACAACTCCGGTGACCGGTTGCCGGTGCATGTGGATTGGAGGTTGAAAGGTGCTGTTACTCACATCAAAGATCAAGGAAGTTGTGGAAGTTGTTGGGCATTCTCAACAATAGCCACAGTGGAAGCTATAAACAAGATAGTCACAGGGAAGCTTGTGTCTTTGTCAGAACAAGAGCTTGTAGATTGTGACAGAGCTTTCAATGAAGGATGCAATGGTGGTCTCATGGACTATGCCTTTGAGTTCATCATTGGAAATGGTGGCATAGACTCTGATCAAGACTATCCATACAAGGGATTTGAGGGTAGATGTGATCCAACAAGGAAAAATGCCAAGGTAGTGACTATTGATGGATATGAGGATGTTCCATCAAATAATGAAAATGCATTAAAGAAAGCTGTGGCTCATCAACCTGTCAGCGTTGCTATTGAGGCTTCTGGAAGGGCTTTGCAGCTCTATCAATCTGGTGTATTTACTGGTAGATGTGGAACCAGCTTAGATCATGGTGTGGTGGTTGTTGGATATGGTTCTGAAAATGGTAAAGATTATTGGTTGGTGAGGAACTCATGGGGGACTAACTGGGGTGAAGATGGTTACTTCAAGATGGAGCGTAATGTGAAAAGAACTTATACAGGAAAGTGTGGAATCGCAGTTGAAGCTTCATACCCGGTGAAGTA
TARGET=89,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1133
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.109754
PRIMER_LEFT_SEQUENCE=TCTTAGCCAAACACAATCATGG
PRIMER_RIGHT_SEQUENCE=CGACCATTTGGTATGTTTGATG
PRIMER_LEFT=39,22
PRIMER_RIGHT=139,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=0.1247
PRIMER_LEFT_1_PENALTY=0.014945
PRIMER_RIGHT_1_PENALTY=0.109754
PRIMER_LEFT_1_SEQUENCE=ACCAAAACCTTTAACCCATCCT
PRIMER_RIGHT_1_SEQUENCE=CGACCATTTGGTATGTTTGATG
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=139,22
PRIMER_LEFT_1_TM=59.985
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.1282
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=TCTTAGCCAAACACAATCATGG
PRIMER_RIGHT_2_SEQUENCE=CCACTCCTCGTACATGGTCATA
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=176,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=138
PRIMER_PAIR_PENALTY_3=0.1396
PRIMER_LEFT_3_PENALTY=0.014945
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=ACCAAAACCTTTAACCCATCCT
PRIMER_RIGHT_3_SEQUENCE=CCACTCCTCGTACATGGTCATA
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=176,22
PRIMER_LEFT_3_TM=59.985
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.2306
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.226971
PRIMER_LEFT_4_SEQUENCE=TCTTAGCCAAACACAATCATGG
PRIMER_RIGHT_4_SEQUENCE=GGTGTTTTACCAACCACTCCTC
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=189,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=59.773
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_02945 Ps_contig_mira-and-tgicl-ass_franssen_26953
SEQUENCE=GTTCGGGGGTTGTTCTGCGCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCGGACCTTTGCCGGAGAATGGTCTCGGCAAGCTCCGAAAACACCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGAATCAGAGGGTGGAAACGTGAGATCCTAGGGTGGGATTCGTTAGAGGGTGCAGTGAAAGTGACACGTAGTATCATGATAGTGAAACCAGCG
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3176
PRIMER_LEFT_PENALTY=0.231280
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=GTTCTGCGCTTTCCATATTCTC
PRIMER_RIGHT_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT=11,22
PRIMER_RIGHT=209,22
PRIMER_LEFT_TM=60.231
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.3621
PRIMER_LEFT_1_PENALTY=0.275745
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_1_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=209,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.5838
PRIMER_LEFT_2_PENALTY=0.497481
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=GTTGTTCTGCGCTTTCCATATT
PRIMER_RIGHT_2_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=209,22
PRIMER_LEFT_2_TM=60.497
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.6994
PRIMER_LEFT_3_PENALTY=0.275745
PRIMER_RIGHT_3_PENALTY=0.423703
PRIMER_LEFT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_3_SEQUENCE=ATTCCTTGTCGGAGATAGATCG
PRIMER_LEFT_3=33,22
PRIMER_RIGHT_3=150,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.576
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=0.8306
PRIMER_LEFT_4_PENALTY=0.231280
PRIMER_RIGHT_4_PENALTY=0.599276
PRIMER_LEFT_4_SEQUENCE=GTTCTGCGCTTTCCATATTCTC
PRIMER_RIGHT_4_SEQUENCE=GTCACTTTCACTGCACCCTCTA
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=210,22
PRIMER_LEFT_4_TM=60.231
PRIMER_RIGHT_4_TM=59.401
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_02946 Ps_contig_mira-and-tgicl-ass_franssen_26960
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTTACTGTGTACACTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02947 Ps_contig_mira-and-tgicl-ass_franssen_26982
SEQUENCE=TCTGGAAAGTCAAATGAAGTAAATGATGCCAAATATTACGGTGGAGGTTATGGTCATGACTGTGGTCATGGACATGGAGGCTACGGTCATGGAGGCTACGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTAGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGCGGTGGACACAATGGTGGTGGACACTATGGAGGTGGACACTATGGTGGTGGTCACTACGGTCATGGTGGTGGTCATCATGGTGGACATGGTGGTGCTTCCGACAATGGTAACTGATGATACCATGATAA
TARGET=151,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7787
PRIMER_LEFT_PENALTY=0.482784
PRIMER_RIGHT_PENALTY=0.295944
PRIMER_LEFT_SEQUENCE=ATTACGGTGGAGGTTATGGTCA
PRIMER_RIGHT_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT=34,22
PRIMER_RIGHT=321,22
PRIMER_LEFT_TM=60.483
PRIMER_RIGHT_TM=60.296
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.8619
PRIMER_LEFT_1_PENALTY=0.565974
PRIMER_RIGHT_1_PENALTY=0.295944
PRIMER_LEFT_1_SEQUENCE=GATGCCAAATATTACGGTGGAG
PRIMER_RIGHT_1_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=321,22
PRIMER_LEFT_1_TM=60.566
PRIMER_RIGHT_1_TM=60.296
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.9328
PRIMER_LEFT_2_PENALTY=0.667126
PRIMER_RIGHT_2_PENALTY=0.265715
PRIMER_LEFT_2_SEQUENCE=GAGGTTATGGTCATGACTGTGG
PRIMER_RIGHT_2_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=327,22
PRIMER_LEFT_2_TM=59.333
PRIMER_RIGHT_2_TM=59.734
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.9631
PRIMER_LEFT_3_PENALTY=0.667126
PRIMER_RIGHT_3_PENALTY=0.295944
PRIMER_LEFT_3_SEQUENCE=GAGGTTATGGTCATGACTGTGG
PRIMER_RIGHT_3_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_3=43,22
PRIMER_RIGHT_3=321,22
PRIMER_LEFT_3_TM=59.333
PRIMER_RIGHT_3_TM=60.296
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=1.0748
PRIMER_LEFT_4_PENALTY=0.809075
PRIMER_RIGHT_4_PENALTY=0.265715
PRIMER_LEFT_4_SEQUENCE=GGTTATGGTCATGACTGTGGTC
PRIMER_RIGHT_4_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT_4=45,22
PRIMER_RIGHT_4=327,22
PRIMER_LEFT_4_TM=59.191
PRIMER_RIGHT_4_TM=59.734
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_02948 Ps_contig_mira-and-tgicl-ass_franssen_26982
SEQUENCE=TCTGGAAAGTCAAATGAAGTAAATGATGCCAAATATTACGGTGGAGGTTATGGTCATGACTGTGGTCATGGACATGGAGGCTACGGTCATGGAGGCTACGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGGAGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTGGTGGTGGTTACGGTCATGGCGGTAGTGGTGGATACAACGGTGGCGGTGTTTCCAACAATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGCGGTGGACACAATGGTGGTGGACACTATGGAGGTGGACACTATGGTGGTGGTCACTACGGTCATGGTGGTGGTCATCATGGTGGACATGGTGGTGCTTCCGACAATGGTAACTGATGATACCATGATAA
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7787
PRIMER_LEFT_PENALTY=0.482784
PRIMER_RIGHT_PENALTY=0.295944
PRIMER_LEFT_SEQUENCE=ATTACGGTGGAGGTTATGGTCA
PRIMER_RIGHT_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT=34,22
PRIMER_RIGHT=321,22
PRIMER_LEFT_TM=60.483
PRIMER_RIGHT_TM=60.296
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.8619
PRIMER_LEFT_1_PENALTY=0.565974
PRIMER_RIGHT_1_PENALTY=0.295944
PRIMER_LEFT_1_SEQUENCE=GATGCCAAATATTACGGTGGAG
PRIMER_RIGHT_1_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=321,22
PRIMER_LEFT_1_TM=60.566
PRIMER_RIGHT_1_TM=60.296
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.9328
PRIMER_LEFT_2_PENALTY=0.667126
PRIMER_RIGHT_2_PENALTY=0.265715
PRIMER_LEFT_2_SEQUENCE=GAGGTTATGGTCATGACTGTGG
PRIMER_RIGHT_2_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=327,22
PRIMER_LEFT_2_TM=59.333
PRIMER_RIGHT_2_TM=59.734
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.9631
PRIMER_LEFT_3_PENALTY=0.667126
PRIMER_RIGHT_3_PENALTY=0.295944
PRIMER_LEFT_3_SEQUENCE=GAGGTTATGGTCATGACTGTGG
PRIMER_RIGHT_3_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_LEFT_3=43,22
PRIMER_RIGHT_3=321,22
PRIMER_LEFT_3_TM=59.333
PRIMER_RIGHT_3_TM=60.296
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=1.0748
PRIMER_LEFT_4_PENALTY=0.809075
PRIMER_RIGHT_4_PENALTY=0.265715
PRIMER_LEFT_4_SEQUENCE=GGTTATGGTCATGACTGTGGTC
PRIMER_RIGHT_4_SEQUENCE=CACCATAGTGTCCACCTCCATA
PRIMER_LEFT_4=45,22
PRIMER_RIGHT_4=327,22
PRIMER_LEFT_4_TM=59.191
PRIMER_RIGHT_4_TM=59.734
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_02949 Ps_contig_mira-and-tgicl-ass_franssen_27120
SEQUENCE=AAACTTTTTTTAAAAATTTAATTAACATAAAAATTAGTTGAGAAGAATCAAATCAAGTTNGTCAACAAACTATAGCAGAAAATAAAGGAATCATGATAAATTATTATTGTTGAAACACTAAACTGAAGTGATTTACAAAGAACTCAAGCTAACAAAACAAGCTAAGACTTTTGAGTTACAATTCAAGCAAAGATGATGAAAATGTTACAAGTAAACAACAACCATATCTTTCTGGTAGAAGTGTACACAAAGTAAAAAAAGCTCACAACACATAAGAGATACTGTAATCTACAACCAATTATTACTTTTCTTTCTTTCAATTAGTCTGTCTCAACACCTAATCCATACAACACACCAGAGTATTTCTCTGTTTCGCCATTGAAGAACGTATCCCTCAATTTTCTAAATGCACAAGCAGTGAGTAAACTATCCGATCCTGCTTGATGGCAAACACCAATCCTTTCAACTTCCAACAACTCTGCGTTGANATACCACTGCTT
TARGET=307,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.8319
PRIMER_LEFT_PENALTY=4.648778
PRIMER_RIGHT_PENALTY=0.183145
PRIMER_LEFT_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_SEQUENCE=TATNTCAACGCAGAGTTGTTGG
PRIMER_LEFT=218,25
PRIMER_RIGHT=490,22
PRIMER_LEFT_TM=58.351
PRIMER_RIGHT_TM=59.817
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.50
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=273
PRIMER_PAIR_PENALTY_1=5.0200
PRIMER_LEFT_1_PENALTY=4.648778
PRIMER_RIGHT_1_PENALTY=0.371199
PRIMER_LEFT_1_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_1_SEQUENCE=ATACGTTCTTCAATGGCGAAAC
PRIMER_LEFT_1=218,25
PRIMER_RIGHT_1=390,22
PRIMER_LEFT_1_TM=58.351
PRIMER_RIGHT_1_TM=60.371
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=5.0414
PRIMER_LEFT_2_PENALTY=4.648778
PRIMER_RIGHT_2_PENALTY=0.392624
PRIMER_LEFT_2_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_2_SEQUENCE=AGTTGAAAGGATTGGTGTTTGC
PRIMER_LEFT_2=218,25
PRIMER_RIGHT_2=467,22
PRIMER_LEFT_2_TM=58.351
PRIMER_RIGHT_2_TM=60.393
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=5.2475
PRIMER_LEFT_3_PENALTY=4.648778
PRIMER_RIGHT_3_PENALTY=0.598673
PRIMER_LEFT_3_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_3_SEQUENCE=GCAGAGTTGTTGGAAGTTGAAA
PRIMER_LEFT_3=218,25
PRIMER_RIGHT_3=481,22
PRIMER_LEFT_3_TM=58.351
PRIMER_RIGHT_3_TM=59.401
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=5.2780
PRIMER_LEFT_4_PENALTY=4.648778
PRIMER_RIGHT_4_PENALTY=0.629225
PRIMER_LEFT_4_SEQUENCE=CAACCATATCTTTCTGGTAGAAGTG
PRIMER_RIGHT_4_SEQUENCE=GGAAGTTGAAAGGATTGGTGTT
PRIMER_LEFT_4=218,25
PRIMER_RIGHT_4=470,22
PRIMER_LEFT_4_TM=58.351
PRIMER_RIGHT_4_TM=59.371
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_02950 Ps_contig_mira-and-tgicl-ass_franssen_27136
SEQUENCE=TTTAGATAAAAACACTTTATTTTATTACTATTATTACCCAAAAACATACACATACACTTATTATATTATATTATATGTATATGTTTGGAAAGCCCATCAAAGCTGATAGTAATGATTTAATTTCCAATCCTGGCTCCAGTGTTGATCTTGGCCTTTCCAAAATTCTGAGTTCCTGCAGTGTTGTTGAATGCATGTTTGGTTCCATTGTTACTAAGATTAGTGTAACCTCCATTGGTACCCGACGTACTCTGCGTTTGTATTACCTACTTGTCTTTT
TARGET=59,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02951 Ps_contig_mira-and-tgicl-ass_franssen_27289
SEQUENCE=TGTTTGGTAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTACTTGTGTACACTTAA
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02952 Ps_contig_mira-and-tgicl-ass_franssen_27293
SEQUENCE=TTCTCAAGTCTTGAAGGAGCTTGATGTAAGTTTGTTTGTCCGCTTACCCTCAAGCTTTCGTTCGTATCATCGGTTTCGACAACGTTCGTCAAGTTCAATGCATCAGCTTCATTGCACACACACACCAGAATCCTACTAAGTTCACTGCATTGGAGCTTCCTATTTATATGTTATGCTTTTAAGTTCCTTTTGTTGTGTATTTTTATAATTTCTTGTTTTTGGATTTCAAATTGCAAATGGGATGTATAAGAAGTTAATGAA
TARGET=115,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0779
PRIMER_LEFT_PENALTY=0.039178
PRIMER_RIGHT_PENALTY=1.038744
PRIMER_LEFT_SEQUENCE=TGATGTAAGTTTGTTTGTCCGC
PRIMER_RIGHT_SEQUENCE=AAGCTCCAATGCAGTGAACTTAG
PRIMER_LEFT=21,22
PRIMER_RIGHT=157,23
PRIMER_LEFT_TM=60.039
PRIMER_RIGHT_TM=59.961
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=1.3625
PRIMER_LEFT_1_PENALTY=0.039178
PRIMER_RIGHT_1_PENALTY=1.323324
PRIMER_LEFT_1_SEQUENCE=TGATGTAAGTTTGTTTGTCCGC
PRIMER_RIGHT_1_SEQUENCE=AGCTCCAATGCAGTGAACTTAG
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=156,22
PRIMER_LEFT_1_TM=60.039
PRIMER_RIGHT_1_TM=58.677
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=1.4023
PRIMER_LEFT_2_PENALTY=0.363508
PRIMER_RIGHT_2_PENALTY=1.038744
PRIMER_LEFT_2_SEQUENCE=CTTTCGTTCGTATCATCGGTTT
PRIMER_RIGHT_2_SEQUENCE=AAGCTCCAATGCAGTGAACTTAG
PRIMER_LEFT_2=54,22
PRIMER_RIGHT_2=157,23
PRIMER_LEFT_2_TM=60.364
PRIMER_RIGHT_2_TM=59.961
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=1.6733
PRIMER_LEFT_3_PENALTY=0.634599
PRIMER_RIGHT_3_PENALTY=1.038744
PRIMER_LEFT_3_SEQUENCE=TCAAGCTTTCGTTCGTATCATC
PRIMER_RIGHT_3_SEQUENCE=AAGCTCCAATGCAGTGAACTTAG
PRIMER_LEFT_3=49,22
PRIMER_RIGHT_3=157,23
PRIMER_LEFT_3_TM=59.365
PRIMER_RIGHT_3_TM=59.961
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=1.6868
PRIMER_LEFT_4_PENALTY=0.363508
PRIMER_RIGHT_4_PENALTY=1.323324
PRIMER_LEFT_4_SEQUENCE=CTTTCGTTCGTATCATCGGTTT
PRIMER_RIGHT_4_SEQUENCE=AGCTCCAATGCAGTGAACTTAG
PRIMER_LEFT_4=54,22
PRIMER_RIGHT_4=156,22
PRIMER_LEFT_4_TM=60.364
PRIMER_RIGHT_4_TM=58.677
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_02953 Ps_contig_mira-and-tgicl-ass_franssen_27299
SEQUENCE=AAATCACATACAAGCTGTTTGGTGGAGATATTGACAACTACACTGTTTTTAAGCTGATTCTAGAAGTGATTAATAAGGCTGATGGCACCGCTGCTGTTAAATGGACTATTGATTATGTGAAAATCAATGAAGATGTTGATACTCCAAATGGCTGGATGGATTACCTTAGCAAAAGCACTAGAGACATTGATGGTCATCTTCTCAAGGGAGAAAGAGTTGCACTATAAGTCAATAAAAGACATAATATAATTAAATAAAAGTGTGTGTTTGTCTTTTATGGATTATTTATTTATTATATTATTCCATTTNCTTTAATTAATTTATTTGTGTATTTGGTTTTAGTAA
TARGET=282,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02954 Ps_contig_mira-and-tgicl-ass_franssen_27408
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTGTGTACACTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02955 Ps_contig_mira-and-tgicl-ass_franssen_27433
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATCTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTNTAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTGTGTACACTT
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02956 Ps_contig_mira-and-tgicl-ass_franssen_27449
SEQUENCE=CCATGACCGTAACCAACCAACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGACCGTAGCCTCCATGTCCATGACCATAGCCATGACCATAACCTCCACCGTAATATTTGGCATCATTTACTTCATTTGACTTTTCAGCAACTTCCTCTTTGGCATTAGTGGAAGTCTCAGCTAATTCTCTAGCTGAAACCTCGGAAGGAAATAACAAGAACCATGGACCAATAGACCAAGGATGAAGGTAAAACGTTTCTGGAATCCTATTGTACTGAACTCAAGATGAANTNARATGAGATA
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02957 Ps_contig_mira-and-tgicl-ass_franssen_27449
SEQUENCE=CCATGACCGTAACCAACCAACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGACCGTAGCCTCCATGTCCATGACCATAGCCATGACCATAACCTCCACCGTAATATTTGGCATCATTTACTTCATTTGACTTTTCAGCAACTTCCTCTTTGGCATTAGTGGAAGTCTCAGCTAATTCTCTAGCTGAAACCTCGGAAGGAAATAACAAGAACCATGGACCAATAGACCAAGGATGAAGGTAAAACGTTTCTGGAATCCTATTGTACTGAACTCAAGATGAANTNARATGAGATA
TARGET=38,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02958 Ps_contig_mira-and-tgicl-ass_franssen_27505
SEQUENCE=GTCATTGATAACTTTGGTAACAAAATCTCAGAATAGTTAGAATGGCTTTATATCGCACTAAAGAACTAGTCTCCCTTGTTTCAATCATGTTTGTTTTCATGTTGATGATGATGATGTTATTGCACGTTATTGTTGTTGTTGTTGTTGTTGCAGGTTGATAGATTGAACAGTGAGGGTTATTATGGTGGAGTTCGATTGCTTATGGCTATTTATAAAGTTTTTATWATTATTGTAAAGAGAATGAG
TARGET=103,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02959 Ps_contig_mira-and-tgicl-ass_franssen_27505
SEQUENCE=GTCATTGATAACTTTGGTAACAAAATCTCAGAATAGTTAGAATGGCTTTATATCGCACTAAAGAACTAGTCTCCCTTGTTTCAATCATGTTTGTTTTCATGTTGATGATGATGATGTTATTGCACGTTATTGTTGTTGTTGTTGTTGTTGCAGGTTGATAGATTGAACAGTGAGGGTTATTATGGTGGAGTTCGATTGCTTATGGCTATTTATAAAGTTTTTATWATTATTGTAAAGAGAATGAG
TARGET=130,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02960 Ps_contig_mira-and-tgicl-ass_franssen_27555
SEQUENCE=CCGTCGCCGTCACCTACTGCAAGCGCGGCCGTGGCTTAATCAAGATCAACGGCGCCCCCATCGAGTTAGTCGAGCCGGAGATCCTCAGGTTCAAGGCCTTTGAACCAATCCTGTTGCTGGGAAAGAGCCGTTTCGCTGGAGTGGATATGCGTATTCGTGTCAAAGGCGGTGGTAAAACCTCTCAGATTTACGCCATCAGGCAGAGCATTGCCAAGGCGCTGGTTGCTTTCTACCAGAAGTATGTTGATGAACAGAGCAAGAAGGAGATCAAGGATATTCTTGTTCGTTACGATCGAACTTTGCTTGTTGCTGATCCTAGGCGTTGTGAGCCGAAGAAGTTTGGTGGGCGTGGTGCTCGTGCGAGGTTTCAGAAATCTTATCGTTAGATTATTTATTTGGGAGGAAAAAGAAAGAGGTGTTTTTGGTTTTACTATAATTCAGTTTTCGGTTTCATGTTTTGTTGCTGTGGAAAACTTTTCTTGTTATTCCTACTACCATTGTTTTGTACATTATGTTTGTTTGTTTTAGTTTGAAATTTTAATTTTAGGTTTTGTTATGCGATAACTAATCTCATAAACTTAACTACCCTACCTAAG
TARGET=513,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02961 Ps_contig_mira-and-tgicl-ass_franssen_27590
SEQUENCE=CANAATACTTTTTAGTTTTAAGTTATGAAATCTATCNTYTGTTACTTTTAAAAAAAACATCTCTTTGGATTCAATAGTAATGAATGAAAATTATCCCACACCTATGAAGATTAACATATTGATTGATGCATGGTTATATAAACAAAGATCATATTTTTCTTGAAATCCACACAACATTTATAGAACAGAACAGAACAGAAGGAACTATGTCCACAATAATTTGATGAGTATATCCTTCACCTACATGCAATATTGTTACAACTTAAATATCAACTTGGCAGAGTCTCTATTGTGACAGAGCACTTGTGTTGTAGACAAGGAGTGCACCACCAGCTAGAAGACCGGCTAGAGTTACTGCCCAGATTGCCAGCCCAGTCGTACCTCCGGCGTAGACATCACCGGTGGCAGACCATTCCTTGGGCTCGTAGATAGGGCTGTATCCATCAACATTAGCACCATATTTGTCTACAAACTGGTAAACACCCTTTCCCCTTTGTTTCCTGCCAGAAGCATCAAGGCCATTAGGCAAATCCATGCCACCGCCAGTTCCATAAGGTGTGTCAGTCTTAATCTTCTTGACACCACTGGCAACAACTCTGAATTGCCTTGAAATGGAAGGAAGTCCTTTCACGGAAGATTTCTGAACAGTGAAAGGAGTTGGTTTGAGACTCAAAGAAGCCATAACTGAAGAAGCCATTTCTAATTCTTTTTTTGATTGTTTGGAATGAATCGCCCACC
TARGET=182,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02962 Ps_contig_mira-and-tgicl-ass_franssen_27591
SEQUENCE=AATGGTCTGCCACCGGTGATGTCTACGTTCCGGTAGGTTACGTACTGGGCTGGCAATCTGGGCAGTAACTCTAGCCGGTCTTCTAGCTGGTGGTGCACTCCTTGTCTACAACACAAGTGCTCTGTCACAATAGAGACTCTGCCAAGTTGATATTTAAGTTGTAACAATATTGCATGTAGGTGAAGGATATACTCATCAAATTATTGTGGACATAGTTCCTTCTGTTCTGTTCTGTTCTGTTCTATAAATGTTGTGTGGATTTCAAGAAAAATAATGATTCTTTGTTTATATAACCATGCATCAATCAATATGTTAATACGT
TARGET=220,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02963 Ps_contig_mira-and-tgicl-ass_franssen_27609
SEQUENCE=AGTCAGCTAACTTTTTGTATTAATTTTCATTGCTATAGACTAATGTNGCTGCAATATTTAGTAGCTAAACATATGAAAAAGAATAARRAAAACAATAAAAGAGTATTGAATTTAACATTGTTATAGTAATAATAATAAAGCAAGATTCATAAAATACAGCATGTAATTACATCCATTGTCTCATTAGCACACAAAAGCAACAGAAACTATGAAAGAGAGTTAATCTGTTTACTTAACAAAGTATGGAAGATCCAAAACATAAAGAAGAAAAAAAGCCCAAGTAACACCAGAAATTCCTCCAAAGAAAAACCCACCAGTAAACTTGGCCCATCCATCTGCTGTTTGGAGCTGATCAGGCTGCTTCTTCCGGCCCGTCAAAGTCAATGACGGAGCAGTTGATGGATCACCTTCGTTGAACGATGAAATTCCATAGATCGTAAGACAAATGCTAAGGATAACAACAAGCCCAGCAGCGGCTAAAGAGCCCGCCGCCCCGGCGTATTCGGTGTTCCTTAAAGGCCCGGCTTTCACAAACGGGCCTACGAGGAAGAACCCATGGGCTAAACCCACTTCAATACCCCGGAGTAGTGGGTTCACTGCAGTTCTATAGCCTGGTAGGTTGGAGAGGTACCATGCCACCAATGGGCTTGATGTCACCGGAGTCTCCAGGCTTCCGATGAACGGGTCGCCGTTGATGGGCTGAACCACTTGGTATGTGGGCTTTTCAGATTGGACGGCTTTAACAGTGAAGTTAAATTGTCTTCTAGAAGGCAATTGATGAAGTGATGAAGAGCCACAGAGTCCCTTGGGTGTGACCAAAGGTCTGGTGAAGGTGGACTTGAGTTGGCTTGCCATAGGAGAAGCAGCTGCCATGGCTGAAGTTTTGATGATTATTTCACAACTTGTTGAAATTCCC
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02964 Ps_contig_mira-and-tgicl-ass_franssen_27611
SEQUENCE=TTTTGAATTTAACATTGTTATAGTAATAATAATAAAGCAAGATTCAATAAAAATACAGCATGTAATTACATCCATTGTCTCATTAGCACAACAAAAGCAACAGAAACTATGAAAGAGAGTTAATCTGTTTAACTTAAACAAAGTATGGAAGATCCAAACAATAAAGAAAGAAAAAAGACCCAAGTAACAACCAGAAATTCCTACCAAAACGAAAAACCCAACCAGTAA
TARGET=24,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02965 Ps_contig_mira-and-tgicl-ass_franssen_27613
SEQUENCE=AAGTATAAAATCCATTCAACTAATATTTTTTATTTTTGAAGAAACATTAGGGTAAGAAACAATGGCCGACCCAAAAAGAGAAATGATCATCAAACATGATATAATTTAACAGACAACAGATAGCCAATGTTACCAAATCTAAACACCAAAAAGAAATCAGCAAGCAAATTTAAAGGGATACAAGAGCCGTTTTTGAAAATTTTAAATGTTTCTAGCAATCAAGAGGGGGAGAAGAAGGAAGGTCCTTAAGAATCCAATAATCCACAAGGGGGAAAGCAACTCCGGCAGAACAGTACAGTCACAATGGACATATATATTGCAGTGAGATCTTAGCTATATCCCATTTGCTGTTGTTGCTGCTGGGGGTGTTGGTAACCAGCATAACCCGGATAACTTCCATACACATTGGGATCTTGTCCAGCAGGAGCAGCAGCATAACCATAATTTTCATAACCACCCTGAGGATAACCATAATATCCACCACCTGCAGCAGCACTCCACTGGTTTGGATCTTGTGGAGTCTGTTTGTTTGAGGGAGTACGACCCCATGAAAGGCGGACATTTTGTCCACCCAATAGAGTTCCATTTAAAACTCTAATTGTTTCTTCTGCACAGCTCCTGTCAGAAAATTGGACAAACCCACATCGCTTGCCAGATGGAATCTTCACATGAACTAGTTCACCATATTGGCTGAACACTTGCCTCAGATGATCATCAGTGACATTAGGATCCAAATTGCCAACAAAAATAGTTGTATTGTTAGGATCATTCTCGCTTTGTCCTCCTAGAGGGTTCTGATAAGAGGCTTTGGGCTGAGTGGTGGCAGCCGGGTTCTTATTTGTGGCTGGTCCAATCCTCATGGGTCTTGTGGAACAAAGAACCCCTTGCATCTCAGTCATAGCCCTCATCTGTTCACCCTCATCAGCAAATCTCACAAAACCATAACCCTTAGACCTGCCAGTGAGTCTATCAATCACAACTTTCGCCCCCTTGACAGAGTTAAAACGAGCCCTAAAAGTCTCCTGAAGAAGGTAATCACTAACATCTGCAGCCAAATCGCCGACAAAAATGGTATAATCAGGACCTTCATCCTGCCTAGACGACCTCTCCCCAGCACTGAAAGTAGCCCAATTCAATCTAAAGCTCTGGCCACCATTCGGCATAATAGTTCCGTTGTATGTCTGAAGCACTCTCTCAGCATTAGCACGGGTATTAAACTCAATAAAACCATAACCTTCCGATTGACTAGTTTGCTTATTCCTAATCACTTTCACAGATGTCACCTCGCCGGTATTACCGAAGCAGGTGTAGAGGTAGTTTTCGTCCATCCAGTACTGTAAATCTCCGATCCAGAGAGTACGGACCTCGTCGGCGCTGGCTGGCTGCTGAGCAGGCTGGGCGGATTGGGCCCACAAGGCTTGCGGCGCTTGAGGTTGCATCATGACGTACGGTTGCTGCGGTGGAGGCTGCTGGTATTGCTGCGGTGGCTGTTGTCCCAAGCTTGGAGGAGCCATACCATGTCCAGGCTGCATCATCTTTCTCACCGAATCTGAAATTCTAGAGAGAGAAAGGGGAGCAGAAGTGAGCGAAGAGAGAGGGAGAGAATTTCGTGTCTTGTTGTGTATTTAAACAATTCTCAAACCCTAACCCTAAAATAATGATACTGATGCAATGGATCCNCGCGTACTTTGCGTTGATACCCCTNNNGCTT
TARGET=1374,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0221
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.019797
PRIMER_LEFT_SEQUENCE=AGAGGTAGTTTTCGTCCATCCA
PRIMER_RIGHT_SEQUENCE=ACTTCTGCTCCCCTTTCTCTCT
PRIMER_LEFT=1309,22
PRIMER_RIGHT=1582,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.020
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.0221
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.019797
PRIMER_LEFT_1_SEQUENCE=GAGGTAGTTTTCGTCCATCCAG
PRIMER_RIGHT_1_SEQUENCE=ACTTCTGCTCCCCTTTCTCTCT
PRIMER_LEFT_1=1310,22
PRIMER_RIGHT_1=1582,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.1186
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.116250
PRIMER_LEFT_2_SEQUENCE=AGAGGTAGTTTTCGTCCATCCA
PRIMER_RIGHT_2_SEQUENCE=ATTCTCTCCCTCTCTCTTCGCT
PRIMER_LEFT_2=1309,22
PRIMER_RIGHT_2=1605,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=60.116
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=0.1186
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.116250
PRIMER_LEFT_3_SEQUENCE=GAGGTAGTTTTCGTCCATCCAG
PRIMER_RIGHT_3_SEQUENCE=ATTCTCTCCCTCTCTCTTCGCT
PRIMER_LEFT_3=1310,22
PRIMER_RIGHT_3=1605,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=60.116
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.1879
PRIMER_LEFT_4_PENALTY=0.168055
PRIMER_RIGHT_4_PENALTY=0.019797
PRIMER_LEFT_4_SEQUENCE=ACCGAAGCAGGTGTAGAGGTAG
PRIMER_RIGHT_4_SEQUENCE=ACTTCTGCTCCCCTTTCTCTCT
PRIMER_LEFT_4=1295,22
PRIMER_RIGHT_4=1582,22
PRIMER_LEFT_4_TM=59.832
PRIMER_RIGHT_4_TM=60.020
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_02966 Ps_contig_mira-and-tgicl-ass_franssen_27633
SEQUENCE=AGCAGTCCTTGTCATAACCGAAACTAGTTGTATAACTGTTTTCCTACCAANCACTGGTGCTNAGNTCNCTAAGANGTTTGCAACAGCTCAGCATTTGTANCGAAACCTCCCTCTGAATCAGTTTGTCCAAAGAAGGCTTGTTTTCCAAGCAAATAGTCCACCTTAGATAGGAACTCTTCAGGTGAACCATAGTCAGTGATGGACTTCTTATCAGTTGTTTGGACTAAAACCGAAACATTGCTTGTTGCATCAAAATTATCCTCATATCTAAGAACCTGACCAGGGAACTCTCTCTCTTTGCTTGGATTACATTTTGCTTACACCAGCAGCTTGTAATCCATCTCCATTGTTATGGCGAAGTAAATCTTGTGTTTGTTCTTTCG
TARGET=288,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1574
PRIMER_LEFT_PENALTY=0.110131
PRIMER_RIGHT_PENALTY=0.047267
PRIMER_LEFT_SEQUENCE=CTCCCTCTGAATCAGTTTGTCC
PRIMER_RIGHT_SEQUENCE=GCCATAACAATGGAGATGGATT
PRIMER_LEFT=106,22
PRIMER_RIGHT=355,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=60.047
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=0.1574
PRIMER_LEFT_1_PENALTY=0.110131
PRIMER_RIGHT_1_PENALTY=0.047267
PRIMER_LEFT_1_SEQUENCE=CCTCCCTCTGAATCAGTTTGTC
PRIMER_RIGHT_1_SEQUENCE=GCCATAACAATGGAGATGGATT
PRIMER_LEFT_1=105,22
PRIMER_RIGHT_1=355,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.047
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=0.1752
PRIMER_LEFT_2_PENALTY=0.127927
PRIMER_RIGHT_2_PENALTY=0.047267
PRIMER_LEFT_2_SEQUENCE=GGCTTGTTTTCCAAGCAAATAG
PRIMER_RIGHT_2_SEQUENCE=GCCATAACAATGGAGATGGATT
PRIMER_LEFT_2=134,22
PRIMER_RIGHT_2=355,22
PRIMER_LEFT_2_TM=60.128
PRIMER_RIGHT_2_TM=60.047
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.3161
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.315379
PRIMER_LEFT_3_SEQUENCE=TTTCCAAGCAAATAGTCCACCT
PRIMER_RIGHT_3_SEQUENCE=AAGCTGCTGGTGTAAGCAAAAT
PRIMER_LEFT_3=141,22
PRIMER_RIGHT_3=331,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.315
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=0.3161
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.315379
PRIMER_LEFT_4_SEQUENCE=TTCCAAGCAAATAGTCCACCTT
PRIMER_RIGHT_4_SEQUENCE=AAGCTGCTGGTGTAAGCAAAAT
PRIMER_LEFT_4=142,22
PRIMER_RIGHT_4=331,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=60.315
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_02967 Ps_contig_mira-and-tgicl-ass_franssen_27637
SEQUENCE=GCGGGAAATGTTACAAATCATCCATTCAAATCCAATATCCAAAACCAACCACRTGTTCATTGCCACATCAGATACACCATATCTAAATCCAATTCAAACTTCACAAACACTAAAAGCCTTTACTCTTAGAGTGTCAAATTCACAAGAAAAAAATTAAACTATAGCATACTCTTAAGAAAAATGGCATCTACACAATGTTTCTTGCACCACCAATATGCTATTACAACTCCAACTAGAACCTTATCTCAACGCCAAGTAGTTACCACCAAACCAAACCATATTGTTTGCAAGGCACAGAAACAAGATGATGTTGTTGATGCTGTTGTGTCTCGTAGGTTAGCACTTTCTGTTCTCATTGGTGCTGCTGCTGTTGGCTCCAAGGTTTCACCTGCAGATGCTGCTTATGGAGAAGCTGCTAATGTGTTTGGAAAGGCAAAGACAAACACAGATTACTTGCCATACAATGGAGATGGATTCAAGCTGCTGGTGCCAGCAAAATGGAATCCAAGCAAAGAGAGAGAGTTCCCTGGTCAGGTTCTTAGATATGAGGATAATTTTGATGCAACAAGCAATGTTTCGGTTTTAGTCCAAACAACTGATAAGAAGTCCATCACTGACTATGGTTCACCTGAAGAGTTCCTATCTAAGGTGGACTATTTGCTTGGAAAACAAGCCTTCTTTGGACAAACTGATTCAGAGGGAGGTTTCGATACAAATGCTGTAGCTGTTGCAAACATCTTAGAGAGCTCAGCACCAGTGATTGGTGGAAAACAGTATTACAACATTTCGGTATTGACAAGGACTGCTGATGGAGATGAAGGTGGAAAGCATCAGCTGATTACAGCAACTGTAAAGGATGGAAAATTATACATCTGCAAGGCTCAAGCTGGTGACAAGAGGTGGTTTAAGGGAGCAAGAAAGTTTGTGGAGGACACTGCAAGTTCTTTCAGTGTTGCCTAATCAAGAACTTTGGTGAAAGGCTGTTATTTTGCATGTAAATCTTATTCTTAAGTAAGCAAGCACTTGGAGTTTTAATTTATAATGGCTTTTTAAATCTGCTTTTTTTAATTTAAAAATTTCAAGTTGTTACAAGCCGTTTAAGAAGCACTATAATATGAAAAATCATTTGTTGGAAGAAATCAACCTCCTTGGAAGCGTGTTGGAAAAGAGTGTATTTTGTATTTTATACATGAAAAATAGTGTTAGTAGTGCTTTTATTYTATATTAAGGAGGCATAAAGGAA
TARGET=513,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02968 Ps_contig_mira-and-tgicl-ass_franssen_27646
SEQUENCE=TGATGGACTTCTTATTCAGTGTTTGGTNTAAACCGAAACAATTGCTTGTTGCATCAAATTAATCTCATATCTAGGAACCTGACCAGGGAACTCTCTCTCTTTGCTTGGATTCCATTTTGCTGGCACCAGCAGCTTGAATCCATCTCCATTGTATGGCAAGTAATCTGTGTTTGTCTTTGCCTTTCCAAACACATTAGCAGCTTCTCCATAAGCAGCATCTGCAGGTNAAACCTTGGANCCAANGCAGCAGCACATGAGAACAGAAGTNCTAACNGANACA
TARGET=91,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0661
PRIMER_LEFT_PENALTY=0.044904
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=TATCTAGGAACCTGACCAGGGA
PRIMER_RIGHT_SEQUENCE=GTTTGGAAAGGCAAAGACAAAC
PRIMER_LEFT=67,22
PRIMER_RIGHT=189,22
PRIMER_LEFT_TM=59.955
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=0.3775
PRIMER_LEFT_1_PENALTY=0.044904
PRIMER_RIGHT_1_PENALTY=0.332592
PRIMER_LEFT_1_SEQUENCE=TATCTAGGAACCTGACCAGGGA
PRIMER_RIGHT_1_SEQUENCE=NACTTCTGTTCTCATGTGCTGC
PRIMER_LEFT_1=67,22
PRIMER_RIGHT_1=267,22
PRIMER_LEFT_1_TM=59.955
PRIMER_RIGHT_1_TM=59.667
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.6386
PRIMER_LEFT_2_PENALTY=0.044904
PRIMER_RIGHT_2_PENALTY=0.593677
PRIMER_LEFT_2_SEQUENCE=TATCTAGGAACCTGACCAGGGA
PRIMER_RIGHT_2_SEQUENCE=GAAGCTGCTAATGTGTTTGGAA
PRIMER_LEFT_2=67,22
PRIMER_RIGHT_2=203,22
PRIMER_LEFT_2_TM=59.955
PRIMER_RIGHT_2_TM=59.406
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=0.6436
PRIMER_LEFT_3_PENALTY=0.044904
PRIMER_RIGHT_3_PENALTY=0.598673
PRIMER_LEFT_3_SEQUENCE=TATCTAGGAACCTGACCAGGGA
PRIMER_RIGHT_3_SEQUENCE=GAAAGGCAAAGACAAACACAGA
PRIMER_LEFT_3=67,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=59.955
PRIMER_RIGHT_3_TM=59.401
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=0.8800
PRIMER_LEFT_4_PENALTY=0.044904
PRIMER_RIGHT_4_PENALTY=0.835083
PRIMER_LEFT_4_SEQUENCE=TATCTAGGAACCTGACCAGGGA
PRIMER_RIGHT_4_SEQUENCE=ACTTCTGTTCTCATGTGCTGCT
PRIMER_LEFT_4=67,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=59.955
PRIMER_RIGHT_4_TM=59.165
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_02969 Ps_contig_mira-and-tgicl-ass_franssen_27753
SEQUENCE=TATATACTACATCAACATTCTTATTACTGTCTAGTTTTATTCTGTCAACCAAAACTGAATAATAATAATAATACTTTTAAATGCAACTTTTAATTATACTGATACAACACAGGTAACAATAATAATAATACTTTTAAATGCAACTTTTAATTATACTAGTACAACACAGGTAACAAACACGAACACTCAAAAGTTACATGCCAAACAAAAGAGACCATTATTATTATTTTCAAACACCATTTGAGTATCAAACAAAGCCTCTGTCCTTTTTATCCCATGCTACAAACAAGCAGAAACCAAATTATGCAGTGGCGTCAACAGTATGGTTGGCAACATCCTTCTCCACAAAATTCTTGATATATTCCCCTCTTGCAGGGAGATGGTCGTGTGGGCGATTGTATGGGGTCCAAACTGGTTCACCAACAAAAAATCGCAAAGCCTTAATATAATTGCCCTCATCCAATGTAAAACGGTGATAAATTCCAGCAGGTAAAATGATCATTCCCCCTTTCTTCACCCATACACGAATCCAAGCCTCATTGCGGTCCCTTACATCAAAGTAACCACTTCCAGCAACAGCATAACGGATCTCCTCATCAATGTGCAGATGCTCTTCAAAGAAGCTCCTGATCTTCTCATCGTAATTTGGCTAACTTTTCGTGGGGAGATCTCAACAAACATCCAAGTAAGANCRACCGCGTTCTTCACGGATTCTTCTTCAGCTCGTGGGTCC
TARGET=58,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02970 Ps_contig_mira-and-tgicl-ass_franssen_27753
SEQUENCE=TATATACTACATCAACATTCTTATTACTGTCTAGTTTTATTCTGTCAACCAAAACTGAATAATAATAATAATACTTTTAAATGCAACTTTTAATTATACTGATACAACACAGGTAACAATAATAATAATACTTTTAAATGCAACTTTTAATTATACTAGTACAACACAGGTAACAAACACGAACACTCAAAAGTTACATGCCAAACAAAAGAGACCATTATTATTATTTTCAAACACCATTTGAGTATCAAACAAAGCCTCTGTCCTTTTTATCCCATGCTACAAACAAGCAGAAACCAAATTATGCAGTGGCGTCAACAGTATGGTTGGCAACATCCTTCTCCACAAAATTCTTGATATATTCCCCTCTTGCAGGGAGATGGTCGTGTGGGCGATTGTATGGGGTCCAAACTGGTTCACCAACAAAAAATCGCAAAGCCTTAATATAATTGCCCTCATCCAATGTAAAACGGTGATAAATTCCAGCAGGTAAAATGATCATTCCCCCTTTCTTCACCCATACACGAATCCAAGCCTCATTGCGGTCCCTTACATCAAAGTAACCACTTCCAGCAACAGCATAACGGATCTCCTCATCAATGTGCAGATGCTCTTCAAAGAAGCTCCTGATCTTCTCATCGTAATTTGGCTAACTTTTCGTGGGGAGATCTCAACAAACATCCAAGTAAGANCRACCGCGTTCTTCACGGATTCTTCTTCAGCTCGTGGGTCC
TARGET=118,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02971 Ps_contig_mira-and-tgicl-ass_franssen_27753
SEQUENCE=TATATACTACATCAACATTCTTATTACTGTCTAGTTTTATTCTGTCAACCAAAACTGAATAATAATAATAATACTTTTAAATGCAACTTTTAATTATACTGATACAACACAGGTAACAATAATAATAATACTTTTAAATGCAACTTTTAATTATACTAGTACAACACAGGTAACAAACACGAACACTCAAAAGTTACATGCCAAACAAAAGAGACCATTATTATTATTTTCAAACACCATTTGAGTATCAAACAAAGCCTCTGTCCTTTTTATCCCATGCTACAAACAAGCAGAAACCAAATTATGCAGTGGCGTCAACAGTATGGTTGGCAACATCCTTCTCCACAAAATTCTTGATATATTCCCCTCTTGCAGGGAGATGGTCGTGTGGGCGATTGTATGGGGTCCAAACTGGTTCACCAACAAAAAATCGCAAAGCCTTAATATAATTGCCCTCATCCAATGTAAAACGGTGATAAATTCCAGCAGGTAAAATGATCATTCCCCCTTTCTTCACCCATACACGAATCCAAGCCTCATTGCGGTCCCTTACATCAAAGTAACCACTTCCAGCAACAGCATAACGGATCTCCTCATCAATGTGCAGATGCTCTTCAAAGAAGCTCCTGATCTTCTCATCGTAATTTGGCTAACTTTTCGTGGGGAGATCTCAACAAACATCCAAGTAAGANCRACCGCGTTCTTCACGGATTCTTCTTCAGCTCGTGGGTCC
TARGET=217,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02972 Ps_contig_mira-and-tgicl-ass_franssen_27807
SEQUENCE=GGTTGGATCTGGTTTTGGTAAGGTTAGGTGGGTAGGCAGCAGTGGTGTTGGTTGGTTGGGTTGGGTACGTCCATACTGATGCAGATAATCAGCAGCCTTATCAAGATACTGTCCTGCGCCCTTATTATCATCCAATTTAGCATACTTTGACCAACTGCATCTAGAAGATCACCGGCAGCTTCTGCCGCTTTGGCTTTGTCTACCTCTGATTCTTTCCCGAGACCTGACTGTGCTGCCTCTGCTACCACCTTTGCGCTTGCCATAAGCTCGCTTGTTGAATACTTCTTGAGCTCTCCAAATCATCTCTCAACTTCATGTCAAGTCCATTGGAGACAACTTGAGAGAATTTACCATCCTTGTAATCCTTCTTTCTATTCAAGAACCAATCAGGTACCTTGAATTGACGAGGGTTGGCAACCACTGTCATCAAATTATCCA
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1453
PRIMER_LEFT_PENALTY=0.135429
PRIMER_RIGHT_PENALTY=0.009871
PRIMER_LEFT_SEQUENCE=TGGTTTTGGTAAGGTTAGGTGG
PRIMER_RIGHT_SEQUENCE=TAATAAGGGCGCAGGACAGTAT
PRIMER_LEFT=9,22
PRIMER_RIGHT=126,22
PRIMER_LEFT_TM=60.135
PRIMER_RIGHT_TM=60.010
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=118
PRIMER_PAIR_PENALTY_1=0.1453
PRIMER_LEFT_1_PENALTY=0.135429
PRIMER_RIGHT_1_PENALTY=0.009871
PRIMER_LEFT_1_SEQUENCE=TGGTTTTGGTAAGGTTAGGTGG
PRIMER_RIGHT_1_SEQUENCE=ATAATAAGGGCGCAGGACAGTA
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=127,22
PRIMER_LEFT_1_TM=60.135
PRIMER_RIGHT_1_TM=60.010
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=119
PRIMER_PAIR_PENALTY_2=0.2688
PRIMER_LEFT_2_PENALTY=0.258908
PRIMER_RIGHT_2_PENALTY=0.009871
PRIMER_LEFT_2_SEQUENCE=GTTGGATCTGGTTTTGGTAAGG
PRIMER_RIGHT_2_SEQUENCE=TAATAAGGGCGCAGGACAGTAT
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=126,22
PRIMER_LEFT_2_TM=59.741
PRIMER_RIGHT_2_TM=60.010
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=5.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.2688
PRIMER_LEFT_3_PENALTY=0.258908
PRIMER_RIGHT_3_PENALTY=0.009871
PRIMER_LEFT_3_SEQUENCE=GGTTGGATCTGGTTTTGGTAAG
PRIMER_RIGHT_3_SEQUENCE=TAATAAGGGCGCAGGACAGTAT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=126,22
PRIMER_LEFT_3_TM=59.741
PRIMER_RIGHT_3_TM=60.010
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=5.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=0.2688
PRIMER_LEFT_4_PENALTY=0.258908
PRIMER_RIGHT_4_PENALTY=0.009871
PRIMER_LEFT_4_SEQUENCE=GGTTGGATCTGGTTTTGGTAAG
PRIMER_RIGHT_4_SEQUENCE=ATAATAAGGGCGCAGGACAGTA
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=127,22
PRIMER_LEFT_4_TM=59.741
PRIMER_RIGHT_4_TM=60.010
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_02973 Ps_contig_mira-and-tgicl-ass_franssen_27819
SEQUENCE=AATTTTAACAAGTTCATCTATGAATTTCTGAACTTTCTTGTTTGTAGTTTCTTTCTTTAGCCCTGCTTCATCTCGTTCTTTGATCACACCCTTGATTTGAGTTGAAGAAGCCTCATATAGTGCAAGAACTTTTGTTTGAAGTTCAGTTTTCTTTTCCTCAAACTCTGTATTGATTCCCTCCTTTGATTCATCAAAGGACTTAGAGGCCTCAGCAGCAGCAGCTTTCTTAGTACCATTCTTCTCAAAACCTTCTTGATTTTAGGAAGTAACTTTTGTTTCCAATAACCCATTATTTTTGCTTACAAAATTNTTTCTCTCTTGTTTCTCTCTAGTGGTTNATAACTAGCTCTTATAACTATACAAAGTCGAACTCAAATTAAACGCCCTACTTCGCCCTTNCTT
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8904
PRIMER_LEFT_PENALTY=1.421170
PRIMER_RIGHT_PENALTY=0.469251
PRIMER_LEFT_SEQUENCE=GATTTGAGTTGAAGAAGCCTCA
PRIMER_RIGHT_SEQUENCE=TAGGGCGTTTAATTTGAGTTCG
PRIMER_LEFT=93,22
PRIMER_RIGHT=387,22
PRIMER_LEFT_TM=58.579
PRIMER_RIGHT_TM=60.469
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=1.8904
PRIMER_LEFT_1_PENALTY=1.421170
PRIMER_RIGHT_1_PENALTY=0.469251
PRIMER_LEFT_1_SEQUENCE=GATTTGAGTTGAAGAAGCCTCA
PRIMER_RIGHT_1_SEQUENCE=CGAAGTAGGGCGTTTAATTTGA
PRIMER_LEFT_1=93,22
PRIMER_RIGHT_1=392,22
PRIMER_LEFT_1_TM=58.579
PRIMER_RIGHT_1_TM=60.469
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=2.2817
PRIMER_LEFT_2_PENALTY=1.812450
PRIMER_RIGHT_2_PENALTY=0.469251
PRIMER_LEFT_2_SEQUENCE=TCTGTATTGATTCCCTCCTTTG
PRIMER_RIGHT_2_SEQUENCE=TAGGGCGTTTAATTTGAGTTCG
PRIMER_LEFT_2=163,22
PRIMER_RIGHT_2=387,22
PRIMER_LEFT_2_TM=58.188
PRIMER_RIGHT_2_TM=60.469
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=2.2817
PRIMER_LEFT_3_PENALTY=1.812450
PRIMER_RIGHT_3_PENALTY=0.469251
PRIMER_LEFT_3_SEQUENCE=CTGTATTGATTCCCTCCTTTGA
PRIMER_RIGHT_3_SEQUENCE=TAGGGCGTTTAATTTGAGTTCG
PRIMER_LEFT_3=164,22
PRIMER_RIGHT_3=387,22
PRIMER_LEFT_3_TM=58.188
PRIMER_RIGHT_3_TM=60.469
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=2.2817
PRIMER_LEFT_4_PENALTY=1.812450
PRIMER_RIGHT_4_PENALTY=0.469251
PRIMER_LEFT_4_SEQUENCE=CTGTATTGATTCCCTCCTTTGA
PRIMER_RIGHT_4_SEQUENCE=CGAAGTAGGGCGTTTAATTTGA
PRIMER_LEFT_4=164,22
PRIMER_RIGHT_4=392,22
PRIMER_LEFT_4_TM=58.188
PRIMER_RIGHT_4_TM=60.469
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_02974 Ps_contig_mira-and-tgicl-ass_franssen_27824
SEQUENCE=TACGTAAGAAAGACTGCTGCTGCTGAGGGCCGTCCTAAGTACCTTTCGTATGAATCAAAGGAAGGGGAAGTCAATACAAGAGTTTTGTATGGAGGAAGGGAAAAACCTGAACTTCAAACAAAAGTTACTTGCACTATATGAGGCTTCTTCAACTCAAATCAAGGGTGTGATCAAAGAACGAGATGAAGCAGGGCTAAAGAAGAACTCAACAGAAGTTCAGAAATTCATAGATGAACTTGTTAAAATTGATTTTCCTGGATCAAAAGCAGTATCTGAAGCATCTTCAGTATTGGNGCGGTCCTTAGTTCAGGTCCTAATTTTC
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02975 Ps_contig_mira-and-tgicl-ass_franssen_27830
SEQUENCE=TCACGTGAAAACAATTTTATTAGGCATTTGGTAACTTAAAACAGAAAGAAAGTTTAAGACGAAAAACAAACCACCATTTGATTAGCTACTAAACAAACTAAAAAATTATCAAAGAGCAAAAACAATCCACCCAAGTTTTATGTAGCGGCGGTTCAGTTCTAATTTCTAGTTATAGCAAAAAGACTTGAAAGTAGTCATCTATATGGAACAGATACAAATGACTAAAAAGGGAGGAAAATGTAATCATCAAGCTGCAGCTGCTTCCTCTTTGAGCAACATCTGATCAAAGTGCCAAACACCATGTCCTTTGCCAACTCTTCTAAGCTGAGCAACATACTCAGATATTTTTTTTATGGCTTCCACCTGTTCACCCAAAAACTGACTTTCCACAAAGTCTGCCAACTGCACATCACCATTTTTTGAGGCAACACTATGCAGGTTAAGGAGTTTTTCATTTGTTAACTTCTCCAATGACAGTGCTAGTTCCATTGCGTGCAGCGCATCACCCTTATCAGCATGTTCAAACTCAGACAACGGCATCACCATAGATTGCAACTTCACTTTTCCACCCCTTTTGTTCTGATATTCCATCAATTTCTCAGCATGCTCTCTTTCCTCTTCACTTGATTCTTTAAAAAATTTAGCAAGACCTTTCAGCGCAACATTATCTCTATCAAAATACGCATACATCGCATGGTAAACATACGAAACATTATACTCCACATTGATTTGTTCGTTAATAGCAGCTTCAGATTCGACATTGAATTTATGGCGAGCTATAGAATCTTGTGGAACAATAGGAACAAGATCGAGTTCCTTTTTCACCTCTTCAAAAGGTTCAAACAATACACCTGTCAGAACACGGTTATCGCTAGAGCCTTTTGTAGCAGAAACAGAGAATTGGTTCAATTTGATTCCTCTGTGAAGAAACGGAAGCGAACGCGAGTTTTCGGAGTTGAAGAGAGAAAGGGAGGAAGAGGAAGCGGTTATGGCGGTTTTGAGAAGCATGATTAGGGATTTTGTACGGAACGGAAATGGAAAGAAAGAAATGAGAGAGAAATTAACAACAAGGGTCTCATTGAGCGCAACCGTAGGAACCCTTTCCAGAGCATTTTCCAACACCTTGATTGAATCAGACAAATCCCTCAAATACATCAAACAAAGAGCTTTATTGTT
TARGET=1038,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3693
PRIMER_LEFT_PENALTY=0.008930
PRIMER_RIGHT_PENALTY=0.360320
PRIMER_LEFT_SEQUENCE=TTCGGAGTTGAAGAGAGAAAGG
PRIMER_RIGHT_SEQUENCE=ATCAAGGTGTTGGAAAATGCTC
PRIMER_LEFT=948,22
PRIMER_RIGHT=1127,22
PRIMER_LEFT_TM=59.991
PRIMER_RIGHT_TM=60.360
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=0.4034
PRIMER_LEFT_1_PENALTY=0.043085
PRIMER_RIGHT_1_PENALTY=0.360320
PRIMER_LEFT_1_SEQUENCE=AGAGAGAAAGGGAGGAAGAGGA
PRIMER_RIGHT_1_SEQUENCE=ATCAAGGTGTTGGAAAATGCTC
PRIMER_LEFT_1=959,22
PRIMER_RIGHT_1=1127,22
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=60.360
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=0.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.4559
PRIMER_LEFT_2_PENALTY=0.095538
PRIMER_RIGHT_2_PENALTY=0.360320
PRIMER_LEFT_2_SEQUENCE=TCAGAACACGGTTATCGCTAGA
PRIMER_RIGHT_2_SEQUENCE=ATCAAGGTGTTGGAAAATGCTC
PRIMER_LEFT_2=854,22
PRIMER_RIGHT_2=1127,22
PRIMER_LEFT_2_TM=59.904
PRIMER_RIGHT_2_TM=60.360
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=0.5459
PRIMER_LEFT_3_PENALTY=0.008930
PRIMER_RIGHT_3_PENALTY=0.536975
PRIMER_LEFT_3_SEQUENCE=TTCGGAGTTGAAGAGAGAAAGG
PRIMER_RIGHT_3_SEQUENCE=GCTCAATGAGACCCTTGTTGTT
PRIMER_LEFT_3=948,22
PRIMER_RIGHT_3=1083,22
PRIMER_LEFT_3_TM=59.991
PRIMER_RIGHT_3_TM=60.537
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=0.5801
PRIMER_LEFT_4_PENALTY=0.043085
PRIMER_RIGHT_4_PENALTY=0.536975
PRIMER_LEFT_4_SEQUENCE=AGAGAGAAAGGGAGGAAGAGGA
PRIMER_RIGHT_4_SEQUENCE=GCTCAATGAGACCCTTGTTGTT
PRIMER_LEFT_4=959,22
PRIMER_RIGHT_4=1083,22
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=60.537
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=0.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_02976 Ps_contig_mira-and-tgicl-ass_franssen_27838
SEQUENCE=GTCGGGGGGCGCGGCGGTCGCTGCAAAACCCAGGGCGCGAGCCTGGGCGGAGCGGTCGNNNGTGGGGATCTTTGTGGTACTTATTTGGAAGAGGCAAGTGGCTTTAATTGAGTTCGACTTTGATAGTTATAAGAGCTTAAGGTTATCAACCACTAGAGAGAAACAGAGAGAAATATTTTGAAGCAAAAATATGGGTTATTGGAAAACAAAAGTTCTTCCTAAAATCAAGAAGGTTTTTGAGAAGAATGGTACTAAGAAAGCTGCTGCTGCTGAGGCCTCTAAGTCCTTTGATGAATCAAAGGAGGGAATCAATACAGAGTTTGAGGAAAAGAAAACTGAACTTCAAACAAAAGTTCTTGCACTATATGAGGCTTCTTCAACTCAAATCAAGGGTGTGATCAAAGAACGAGATGAAGCAGGGACTAAAGAAGAACTCAACAGAAGTTCAAGAAATTCATAGATGAACTTTAGTTAAAATTGTATTTTCCTGGATACAAAAGCAGTATCTGAAGCATCTTACAAAATTTGGAACGGCCTTAGTTTCAGGTCCAATTTTCTTTGTTTTTGAGAAAGTATCAACTTTCATAGTCACAGAAGAAAAAACTGAAGAAGTGGCTAAAACAGAAGAGGAAACAAGCGGTGTAAAAGATAGGGAAATTGTGGTTGAAGAAGGAAAAAAAGAAGAAACAAGTGAATCTGCAGCTGAGAAGGCTGAAGAAAAAGCTAAGGCTGCAAATGACTCTCTGGCTCCGCCGCCGCCGGAGAAAGAGGAAGAGAAACCGGTTGAAACAACTCCGGTGGAAAAAGTGGAACCTCCAAAGGCTTGAAGATCATAAATATGTTGTATATGTGTAATAGTTTGCATATATTCAAAAAAAAATGTTACATACCAAAATCTCTTATTCTTTTATATATTGTGTAATTGAATTTGCTTTGCTTAGTTTGATGCATGTGAATGTGTATTGTGAATCTCTCATTTTGTTAATTTTCTATTTTTGTCTATTTTAATGTCAAAGAATTGTGACTTTGGATTGAATAAAANGGTYTCTTTTAATTCCTTTATTGTT
TARGET=260,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02977 Ps_contig_mira-and-tgicl-ass_franssen_27838
SEQUENCE=GTCGGGGGGCGCGGCGGTCGCTGCAAAACCCAGGGCGCGAGCCTGGGCGGAGCGGTCGNNNGTGGGGATCTTTGTGGTACTTATTTGGAAGAGGCAAGTGGCTTTAATTGAGTTCGACTTTGATAGTTATAAGAGCTTAAGGTTATCAACCACTAGAGAGAAACAGAGAGAAATATTTTGAAGCAAAAATATGGGTTATTGGAAAACAAAAGTTCTTCCTAAAATCAAGAAGGTTTTTGAGAAGAATGGTACTAAGAAAGCTGCTGCTGCTGAGGCCTCTAAGTCCTTTGATGAATCAAAGGAGGGAATCAATACAGAGTTTGAGGAAAAGAAAACTGAACTTCAAACAAAAGTTCTTGCACTATATGAGGCTTCTTCAACTCAAATCAAGGGTGTGATCAAAGAACGAGATGAAGCAGGGACTAAAGAAGAACTCAACAGAAGTTCAAGAAATTCATAGATGAACTTTAGTTAAAATTGTATTTTCCTGGATACAAAAGCAGTATCTGAAGCATCTTACAAAATTTGGAACGGCCTTAGTTTCAGGTCCAATTTTCTTTGTTTTTGAGAAAGTATCAACTTTCATAGTCACAGAAGAAAAAACTGAAGAAGTGGCTAAAACAGAAGAGGAAACAAGCGGTGTAAAAGATAGGGAAATTGTGGTTGAAGAAGGAAAAAAAGAAGAAACAAGTGAATCTGCAGCTGAGAAGGCTGAAGAAAAAGCTAAGGCTGCAAATGACTCTCTGGCTCCGCCGCCGCCGGAGAAAGAGGAAGAGAAACCGGTTGAAACAACTCCGGTGGAAAAAGTGGAACCTCCAAAGGCTTGAAGATCATAAATATGTTGTATATGTGTAATAGTTTGCATATATTCAAAAAAAAATGTTACATACCAAAATCTCTTATTCTTTTATATATTGTGTAATTGAATTTGCTTTGCTTAGTTTGATGCATGTGAATGTGTATTGTGAATCTCTCATTTTGTTAATTTTCTATTTTTGTCTATTTTAATGTCAAAGAATTGTGACTTTGGATTGAATAAAANGGTYTCTTTTAATTCCTTTATTGTT
TARGET=750,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02978 Ps_contig_mira-and-tgicl-ass_franssen_27839
SEQUENCE=CATACCACGCGCCCTCCAATCTTAACCCTCCACGTATCTCTAAATCCAACGGTCACCACGTCTCCAATCACCCAATAAAATCACACACTTATCTCAATCATTCGGGACACACACACGCCACACTCCTCTCAATCTTTTTCCCATTTCCGTTCTGTAGAAAAACCCTAACCATGCTTCTCAAAACCGCCGTAACCGTTTCTTCATCTCCCTCAATTTCTCTCTTCAATTCTGAAAACTCGCTTTTGGTTCCGTTTCTTCACCGAGGAAGTAAACCGGACCGATTAGCGGTTTGTGCTACGAAAGGCTCTAGCAATAACCGTGTTTTAACCGGTGTGTTGTTTGAGCCTTTTGTAGAGGTTAAAAAGGAACTTGATCTTGTTCCTGTTTCTCCACAAGAATCTCTTGCTCGCCATAAATTTCATGGAGAATCTGAAGCTGCTATTAACGAGCAAATCAACGTGGAATATAATGTTTCGTATGTTTACCATACTATGTATGCGTATTTTGATAGAGATAATGTTGCGCTGAAAGGTCTTGCTAAGTTTTTTAAAGAATCAAGTGAAGAGGAAAGAGGGCATGCTGAGAAGTTGATGGAATATCAGAACAAAAGGGGTGGAAAAGTGAAGTTGCAATCTATAGTGACACCGCTATCTGAGTTTGATCATGCTGATAAGGGTGATGCCCTGTATGCAATGGAACTTGCACTGTCATTGGAGAAGCTAACAAATGAAAAACTCCTTAACTTGCACAGTGTTGCCTCAAAGAATGGTGATGTGCACTTGGCTGACTTTGTGGAAAGTGAGTTTTTGGGTGAACAGGTGGAAGCCATCAAAAAAATATCCGAGTATGTTGCTCAGCTCAGAAGAGTCGGCAAAGGACATGGTGTCTGGCACTTTGATCAGGTTCTCGCTAGTGAGAGGAATTGATGATTACATTTTCCTCCTATTTTAGTCATCTGTATATGTTCATATTCAAGTCTTTTTGCTAGAACTAGAAGATGGAACTGAACTATTAGAACTAGAAGTTGGAACTGAACTATGTCTGAATAAAATGAATGTGGATTGCTTTTTATTAGTTGTATTTCAGTTTTAGTAGTTGATCTGTACTGGTTTTTTTTCCACTTAAAAGCTTTCTCTTTCTGTTAAATTTACCAATCACCGGTGAATTGATCAAATGCCTCTTTACG
TARGET=107,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5934
PRIMER_LEFT_PENALTY=0.592680
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=ACGTCTCCAATCACCCAATAAA
PRIMER_RIGHT_SEQUENCE=GTTTTGAGAAGCATGGTTAGGG
PRIMER_LEFT=57,22
PRIMER_RIGHT=183,22
PRIMER_LEFT_TM=60.593
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=127
PRIMER_PAIR_PENALTY_1=0.5934
PRIMER_LEFT_1_PENALTY=0.592680
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=ACGTCTCCAATCACCCAATAAA
PRIMER_RIGHT_1_SEQUENCE=GGTTTTGAGAAGCATGGTTAGG
PRIMER_LEFT_1=57,22
PRIMER_RIGHT_1=184,22
PRIMER_LEFT_1_TM=60.593
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=128
PRIMER_PAIR_PENALTY_2=0.5934
PRIMER_LEFT_2_PENALTY=0.592680
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=ACGTCTCCAATCACCCAATAAA
PRIMER_RIGHT_2_SEQUENCE=TTTGAGAAGCATGGTTAGGGTT
PRIMER_LEFT_2=57,22
PRIMER_RIGHT_2=181,22
PRIMER_LEFT_2_TM=60.593
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.5934
PRIMER_LEFT_3_PENALTY=0.592680
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=ACGTCTCCAATCACCCAATAAA
PRIMER_RIGHT_3_SEQUENCE=TTTTGAGAAGCATGGTTAGGGT
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=182,22
PRIMER_LEFT_3_TM=60.593
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.5934
PRIMER_LEFT_4_PENALTY=0.592680
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=ACGTCTCCAATCACCCAATAAA
PRIMER_RIGHT_4_SEQUENCE=TGAGAAGCATGGTTAGGGTTTT
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=179,22
PRIMER_LEFT_4_TM=60.593
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_02979 Ps_contig_mira-and-tgicl-ass_franssen_27840
SEQUENCE=GGAAGAGGCAAGTGGCTTTAATTGAGTTCGACTTTGATAGTTATAAGAGCTTAAGGTTATCAACCACTAGAGAGAAACAGAGAGAAATATTTTGAAGCAAAAATATGGGTTATTGGAAAACAAAAGTTCTTCCTAAAATCAAGAAGGTTTTTGAGAAGAATGGTACTAAGAAAGCTGCTGCTGCTGAGGCCTCTAAGTCCTTTGATGAATCAAAGGTTTGTTTTCTTAACTTTGAAAATGAAACTATACTTCATATATGTGATGTTGTTTGGTATTTTGTTAAGCAGTGGTATCAACGCAGAGTTTTGAGGAAAAGAAAACTGAACTTCAAACAAAAGTTCTTGCACTATATGAGGCTTCTTCAACTCAAATCAAGGGTGTGATCAAAGAACGAGATGAAGCAGGGCTAAAGAAGAACTCAACAGAAGTTCAGAAATTCATAGATGAACTTGTTAAAATTGATTTTCCTGGATCAAAAGCAGTATCTGAAGCATCTTCAAAATTTGGAACGGCCTTAGTTTCAGGTCCAATTTTCTTTGTTTTTGAGAAAGTATCAACTTTCATAGTCACAGAAGAAAAAACTGAAGAAGTGGCTAAAACAGAAGAGGAAACAAGCGGTGTAAAAGATAGGGAAATTGTGGTTGAAGAAGGAAAAAAAGAAGAAACAAGTGAATCTGCAGCTGAGAAGGCTGAAGAAAAAGCTAAGGCTGCAAATGACTCTCTGGCTCCGCCGCCGCCGGAGAAAGAGGAAGAGAAACCGGTTGAAACAACTCCGGTGGAAAAAGTGGAACCTCCAAAGGCTTGAAGATCATAAATATGTTGTATATGTGTAATAGTTTGCATATATTCAAAAAAAATGTTACATACCAAAATCTCTTATTCTTTTATATATTGTGTAATTGAATTTGCTTTGCTTAGTTTGATGCATGTGAATGTGTATTGTGAATCTCTCATTTTGTAATTTTCATTTTTGTCATTTTAATGTCAAAGAATTGTGACTTTGGATTGAACAATTGGTTCTTTTAATCCTTTACGTTTGTTGTTCATTAAC
TARGET=174,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4005
PRIMER_LEFT_PENALTY=0.206859
PRIMER_RIGHT_PENALTY=0.193666
PRIMER_LEFT_SEQUENCE=AAGAGGCAAGTGGCTTTAATTG
PRIMER_RIGHT_SEQUENCE=TGCGTTGATACCACTGCTTAAC
PRIMER_LEFT=2,22
PRIMER_RIGHT=299,22
PRIMER_LEFT_TM=59.793
PRIMER_RIGHT_TM=60.194
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=0.4502
PRIMER_LEFT_1_PENALTY=0.256503
PRIMER_RIGHT_1_PENALTY=0.193666
PRIMER_LEFT_1_SEQUENCE=GAGGCAAGTGGCTTTAATTGAG
PRIMER_RIGHT_1_SEQUENCE=TGCGTTGATACCACTGCTTAAC
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=299,22
PRIMER_LEFT_1_TM=60.257
PRIMER_RIGHT_1_TM=60.194
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=0.5218
PRIMER_LEFT_2_PENALTY=0.328094
PRIMER_RIGHT_2_PENALTY=0.193666
PRIMER_LEFT_2_SEQUENCE=GGCAAGTGGCTTTAATTGAGTT
PRIMER_RIGHT_2_SEQUENCE=TGCGTTGATACCACTGCTTAAC
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=299,22
PRIMER_LEFT_2_TM=59.672
PRIMER_RIGHT_2_TM=60.194
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=294
PRIMER_PAIR_PENALTY_3=0.8642
PRIMER_LEFT_3_PENALTY=0.670525
PRIMER_RIGHT_3_PENALTY=0.193666
PRIMER_LEFT_3_SEQUENCE=AGGCAAGTGGCTTTAATTGAGT
PRIMER_RIGHT_3_SEQUENCE=TGCGTTGATACCACTGCTTAAC
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=299,22
PRIMER_LEFT_3_TM=59.329
PRIMER_RIGHT_3_TM=60.194
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=1.0191
PRIMER_LEFT_4_PENALTY=0.328094
PRIMER_RIGHT_4_PENALTY=0.691033
PRIMER_LEFT_4_SEQUENCE=GGCAAGTGGCTTTAATTGAGTT
PRIMER_RIGHT_4_SEQUENCE=AAACTCTGCGTTGATACCACTG
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=305,22
PRIMER_LEFT_4_TM=59.672
PRIMER_RIGHT_4_TM=59.309
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=300
=
PRIMER_SEQUENCE_ID=SSR_02980 Ps_contig_mira-and-tgicl-ass_franssen_27840
SEQUENCE=GGAAGAGGCAAGTGGCTTTAATTGAGTTCGACTTTGATAGTTATAAGAGCTTAAGGTTATCAACCACTAGAGAGAAACAGAGAGAAATATTTTGAAGCAAAAATATGGGTTATTGGAAAACAAAAGTTCTTCCTAAAATCAAGAAGGTTTTTGAGAAGAATGGTACTAAGAAAGCTGCTGCTGCTGAGGCCTCTAAGTCCTTTGATGAATCAAAGGTTTGTTTTCTTAACTTTGAAAATGAAACTATACTTCATATATGTGATGTTGTTTGGTATTTTGTTAAGCAGTGGTATCAACGCAGAGTTTTGAGGAAAAGAAAACTGAACTTCAAACAAAAGTTCTTGCACTATATGAGGCTTCTTCAACTCAAATCAAGGGTGTGATCAAAGAACGAGATGAAGCAGGGCTAAAGAAGAACTCAACAGAAGTTCAGAAATTCATAGATGAACTTGTTAAAATTGATTTTCCTGGATCAAAAGCAGTATCTGAAGCATCTTCAAAATTTGGAACGGCCTTAGTTTCAGGTCCAATTTTCTTTGTTTTTGAGAAAGTATCAACTTTCATAGTCACAGAAGAAAAAACTGAAGAAGTGGCTAAAACAGAAGAGGAAACAAGCGGTGTAAAAGATAGGGAAATTGTGGTTGAAGAAGGAAAAAAAGAAGAAACAAGTGAATCTGCAGCTGAGAAGGCTGAAGAAAAAGCTAAGGCTGCAAATGACTCTCTGGCTCCGCCGCCGCCGGAGAAAGAGGAAGAGAAACCGGTTGAAACAACTCCGGTGGAAAAAGTGGAACCTCCAAAGGCTTGAAGATCATAAATATGTTGTATATGTGTAATAGTTTGCATATATTCAAAAAAAATGTTACATACCAAAATCTCTTATTCTTTTATATATTGTGTAATTGAATTTGCTTTGCTTAGTTTGATGCATGTGAATGTGTATTGTGAATCTCTCATTTTGTAATTTTCATTTTTGTCATTTTAATGTCAAAGAATTGTGACTTTGGATTGAACAATTGGTTCTTTTAATCCTTTACGTTTGTTGTTCATTAAC
TARGET=728,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0914
PRIMER_LEFT_PENALTY=0.072552
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=AAAACAGAAGAGGAAACAAGCG
PRIMER_RIGHT_SEQUENCE=GAGTTGTTTCAACCGGTTTCTC
PRIMER_LEFT=595,22
PRIMER_RIGHT=772,22
PRIMER_LEFT_TM=59.927
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.1313
PRIMER_LEFT_1_PENALTY=0.048131
PRIMER_RIGHT_1_PENALTY=0.083165
PRIMER_LEFT_1_SEQUENCE=AAGCTAAGGCTGCAAATGACTC
PRIMER_RIGHT_1_SEQUENCE=CATGCATCAAACTAAGCAAAGC
PRIMER_LEFT_1=698,22
PRIMER_RIGHT_1=928,22
PRIMER_LEFT_1_TM=60.048
PRIMER_RIGHT_1_TM=59.917
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.1586
PRIMER_LEFT_2_PENALTY=0.075444
PRIMER_RIGHT_2_PENALTY=0.083165
PRIMER_LEFT_2_SEQUENCE=AAAAGCTAAGGCTGCAAATGAC
PRIMER_RIGHT_2_SEQUENCE=CATGCATCAAACTAAGCAAAGC
PRIMER_LEFT_2=696,22
PRIMER_RIGHT_2=928,22
PRIMER_LEFT_2_TM=59.925
PRIMER_RIGHT_2_TM=59.917
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.2305
PRIMER_LEFT_3_PENALTY=0.147383
PRIMER_RIGHT_3_PENALTY=0.083165
PRIMER_LEFT_3_SEQUENCE=GAAGAAAAAGCTAAGGCTGCAA
PRIMER_RIGHT_3_SEQUENCE=CATGCATCAAACTAAGCAAAGC
PRIMER_LEFT_3=691,22
PRIMER_RIGHT_3=928,22
PRIMER_LEFT_3_TM=60.147
PRIMER_RIGHT_3_TM=59.917
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.2697
PRIMER_LEFT_4_PENALTY=0.186529
PRIMER_RIGHT_4_PENALTY=0.083165
PRIMER_LEFT_4_SEQUENCE=GCTGAAGAAAAAGCTAAGGCTG
PRIMER_RIGHT_4_SEQUENCE=CATGCATCAAACTAAGCAAAGC
PRIMER_LEFT_4=688,22
PRIMER_RIGHT_4=928,22
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=59.917
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_02981 Ps_contig_mira-and-tgicl-ass_franssen_27850
SEQUENCE=ATACTTTCTCAAAAACAAAAGAAAAATTAGGACCTGAAACTAAGGCGCGTCCAATTTGAAGATGCTTCAGATACTGCTTTTGATCCAGGAAAATCAATTTTAACAAGTTCATCTATGAATTTCTGAACTTCTGTTGAGTTCTTCTTTAGCCCTGCTTCATCTCGTTCTTTGATCACACCCTTGATTTGAGTTGAAGAAGCCTCATATAGTGCAAGAACTTTTGTTTGAAGTTCAGTTTTCTTTTCCTCAAACTCTGTATTGATTCCCTTCCTTTGATTCATCAAAGGACTTAGAGGCCTCAGCAGCAGCAGTCTTTCTTAGTACCATTCTTCTACTAAAAACCTTCTTGTATTTAAGGAATAACTTTTTTCGTTTTACCCAAACTTAAACCCCTAATTT
TARGET=300,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1278
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=1.119576
PRIMER_LEFT_SEQUENCE=CACACCCTTGATTTGAGTTGAA
PRIMER_RIGHT_SEQUENCE=GGGTTTAAGTTTGGGTAAAACG
PRIMER_LEFT=173,22
PRIMER_RIGHT=391,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=58.880
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=1.1330
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=1.119576
PRIMER_LEFT_1_SEQUENCE=TTCTTTAGCCCTGCTTCATCTC
PRIMER_RIGHT_1_SEQUENCE=GGGTTTAAGTTTGGGTAAAACG
PRIMER_LEFT_1=141,22
PRIMER_RIGHT_1=391,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=58.880
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=1.1408
PRIMER_LEFT_2_PENALTY=0.021195
PRIMER_RIGHT_2_PENALTY=1.119576
PRIMER_LEFT_2_SEQUENCE=TTGAGTTCTTCTTTAGCCCTGC
PRIMER_RIGHT_2_SEQUENCE=GGGTTTAAGTTTGGGTAAAACG
PRIMER_LEFT_2=133,22
PRIMER_RIGHT_2=391,22
PRIMER_LEFT_2_TM=60.021
PRIMER_RIGHT_2_TM=58.880
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=1.1488
PRIMER_LEFT_3_PENALTY=0.029253
PRIMER_RIGHT_3_PENALTY=1.119576
PRIMER_LEFT_3_SEQUENCE=TTGATCACACCCTTGATTTGAG
PRIMER_RIGHT_3_SEQUENCE=GGGTTTAAGTTTGGGTAAAACG
PRIMER_LEFT_3=168,22
PRIMER_RIGHT_3=391,22
PRIMER_LEFT_3_TM=59.971
PRIMER_RIGHT_3_TM=58.880
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=1.4453
PRIMER_LEFT_4_PENALTY=0.325733
PRIMER_RIGHT_4_PENALTY=1.119576
PRIMER_LEFT_4_SEQUENCE=TGAGTTCTTCTTTAGCCCTGCT
PRIMER_RIGHT_4_SEQUENCE=GGGTTTAAGTTTGGGTAAAACG
PRIMER_LEFT_4=134,22
PRIMER_RIGHT_4=391,22
PRIMER_LEFT_4_TM=59.674
PRIMER_RIGHT_4_TM=58.880
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_02982 Ps_contig_mira-and-tgicl-ass_franssen_27858
SEQUENCE=GTAGAGANTNATAACAAACTTCCACTACTCATGAAATGGTTAGAAATTGGTTCCACAGACAAATGCACACACACACACAGCAAAAGGAATAAAGATACTAATGCTCACACCACTCACTTGCTTAAATACAAGTGGAACTTTTAGGAAATACTAAAATACCCTCTAACAAACTCTCAATAAGTTTCTACAAAATGTGAATTAATTCTAACACAAATTACACAATTGACCGTTTTAAAATAACCAGATTTACACATCCCAAGCGCTAATACTAAATATTAAATATTCGTATTAGAGGTGGAACCACTTATTCAAATGAAGACACACAACGACCTAAATAAGCTAAATGGGCCTGAATATCTTTGCTTTTGGGCCTTTCAAAGCTTGGCAATTAGCCCGTTCTGACTAGGTTTGGCAGGCATGCTATTCTCCCTGTCATCCAAATCACTTGGGCTTGGAGGAACATCTATGGCTGGTGAGGAATTGAAAAACCCATGTGGCATAAGCATAAAACCGATGTGTTCCACGGGCATAACAGGCCAGTCTTCCAATCGAGGAATGTGTGTCACCCCAAATACGTACCAAAGAACGATATCAGCTTCTTCCAGTGGTCGATTTTGCTTAACCCAAGTAGCCAAACCCTCTCCAGCGCGGGGATTTTGATTAGGAAACTCTCCACCAGGATGCATTTCGCCGCATGCATAAGAAGTAACCCAAAGATTGTGCTTCAAGAAAGCTGCTCTTCGCAAAAACTTAGCCTCTGATCCGGCTAAAGGTAAACAATTTGAACCAGGAACTAGTTTGTAACCAGTTAGATTACCAGTTCGGTTCACACTCCTCGTGTTCCTAACAATCCAATGACGAGCAGATAAAGGGTCACAATCACGCATTGCTTCTAATTCTGATTTAAGCAGTTTTTCCTCGGCATAAAATGCATTGTGGTGAACATTATTCTCTCCCGGCTCTTCAACTTTGACATTCACCTCAACAACCTGATTAAATGCTTCACCGGGCTTGCAATCAACTGCCATATCCATACGAGCAACAAAAAAGTGTTGATGGAGAGGCGCATATAATCCAGGAGCAATAGTTGTGCCGTATTTTCGAGTTTCTCCTTGTTGCAATGCACCTAAGCTGAGAATTCCTGTGAGCTTGACCTCAGCTTCTATTTTTCCATCCTGATAAAAATGCCAGTAAAATCCATACTCATAGTTAGCCACAGTGCATACAAAAGACACTGTCAGCCTTCTAGACCGTCGAACTTCAGCTAAACCTGTTCTCCAATCTTGATGCTTCCATAACATACCATGATCTTCTTCATGCATACAAACACAATTCTCTATAGTTTCAACACCTCCATAAAAGTTTGTGAAGTGCGCATTGAAGTACTTGATATAGCCTAAACAATCACAACCCTTCTTGAGTGAATGAGCATTTTTACCGAGGCCATCTTCCCCGGCATCAAAAGCGTTTTTCCTATAGTGAGGATCGTTTGGATCTCCATAAGGGACGACCATCTCAACAAAACTCAATCTGTGGGCCACAGGCCTTCGACCACGACTTCCGTCAACATATGCTACTGAATAAATAACCATACCCTCCCTAGGAGTGAATCCAATACGAAAGTTCCACTTCTGCCATTGAACAAAGTGTCCATTAACACGAAAACTTAGGACCACTACCCGGCCGTGAATAATCTGCGAGGGTTTCG
TARGET=66,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0544
PRIMER_LEFT_PENALTY=1.006228
PRIMER_RIGHT_PENALTY=0.048131
PRIMER_LEFT_SEQUENCE=TGGTTAGAAATTGGTTCCACAG
PRIMER_RIGHT_SEQUENCE=AGTATTAGCGCTTGGGATGTGT
PRIMER_LEFT=36,22
PRIMER_RIGHT=269,22
PRIMER_LEFT_TM=58.994
PRIMER_RIGHT_TM=60.048
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=1.2553
PRIMER_LEFT_1_PENALTY=1.006228
PRIMER_RIGHT_1_PENALTY=0.249074
PRIMER_LEFT_1_SEQUENCE=TGGTTAGAAATTGGTTCCACAG
PRIMER_RIGHT_1_SEQUENCE=GCTTGGGATGTGTAAATCTGGT
PRIMER_LEFT_1=36,22
PRIMER_RIGHT_1=260,22
PRIMER_LEFT_1_TM=58.994
PRIMER_RIGHT_1_TM=60.249
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=1.3851
PRIMER_LEFT_2_PENALTY=1.136029
PRIMER_RIGHT_2_PENALTY=0.249074
PRIMER_LEFT_2_SEQUENCE=AATTGGTTCCACAGACAAATGC
PRIMER_RIGHT_2_SEQUENCE=GCTTGGGATGTGTAAATCTGGT
PRIMER_LEFT_2=44,22
PRIMER_RIGHT_2=260,22
PRIMER_LEFT_2_TM=61.136
PRIMER_RIGHT_2_TM=60.249
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=217
PRIMER_PAIR_PENALTY_3=1.3981
PRIMER_LEFT_3_PENALTY=1.148998
PRIMER_RIGHT_3_PENALTY=0.249074
PRIMER_LEFT_3_SEQUENCE=ATTGGTTCCACAGACAAATGC
PRIMER_RIGHT_3_SEQUENCE=GCTTGGGATGTGTAAATCTGGT
PRIMER_LEFT_3=45,21
PRIMER_RIGHT_3=260,22
PRIMER_LEFT_3_TM=59.851
PRIMER_RIGHT_3_TM=60.249
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=216
PRIMER_PAIR_PENALTY_4=1.4592
PRIMER_LEFT_4_PENALTY=1.006228
PRIMER_RIGHT_4_PENALTY=0.452945
PRIMER_LEFT_4_SEQUENCE=TGGTTAGAAATTGGTTCCACAG
PRIMER_RIGHT_4_SEQUENCE=GGTCGTTGTGTGTCTTCATTTG
PRIMER_LEFT_4=36,22
PRIMER_RIGHT_4=330,22
PRIMER_LEFT_4_TM=58.994
PRIMER_RIGHT_4_TM=60.453
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_02983 Ps_contig_mira-and-tgicl-ass_franssen_27871
SEQUENCE=TCAACGCAGAGTACNCGRGGGACGCACACGCCTCCACACAAACCTCTTCCTGCGTCTCTCTCTCTCTAGCGTTCTCTCATCCTTTCGCTAAAACCCTACGTGAAAGGAAAGTCACCATGGTTGATCAGCACCCTACAATCATGGAGAAGGTCACTGGCCAGATCCATCGTCGATCTGGTGTCTCATCGGGTTATGGTGGAAGCTTCCGTCAACCTGCAATGTTCCAAAAATACTCCTATGGGAATTACTCAAACGCTGCATTGGCACCTGCATGCAGGACAACTGTTGATTTATCTTCAGTTGCTACCAATGCTTCTCCTATGTTTGTTGCTGCTCCAGCGGAAAAGAGCCATTTTCTTATTGACTTTCTCATGGGTGGTGTTTCAGCTGCAGTTTCAAAAACTGCTGCTGCTCCCATCGAGCGTGTTAAGCTTTTGATCCAAAACCAGGATGAGATGATCAAGGCTGGTAGGCTTTCTGAGCCATACAAGGGCATTGGTGATTGTTTTAAAAGAACAACTGCCGATGAGGGTTTGGTTGCTCTATGGAGAGGAAACACTGCCAATGTCATTCGTTATTTCCCCACTCAGGCATTGAACTTTGCATTCAAAGATTACTTCAAGAGGCTTTTCAACTTCAAGAAGGACAGAGATGGTTACTGGAAGTGGTTCGCTGGCAACTTGGCATCCGGAGGTGCTGCTGGTGCCTCATCCCTCTTGTTTGTTTACTCACTCGACTATGCCCGTACCCGTCTTGCCAATGATTCTAAGGCAGCTAAGAAGGGTGGAGAAAGACAATTCAATGGTCTTGTCGATGTCTACAAGAAGACCCTTGCAACTGATGGAGTTGCTGGGCTTTACCGTGGTTTCAACATCTCCTGTGTTGGAGTCATTGTCTACCGTGGTCTCTACTTTGGAATGTACGATTCCCTCAAACCAGTTCTTCTGACTGGAAAATTGCAGGATAGCTTTTTCGCCAGTTTTGGACTCGGATGGCTCATCACCAATGGTGCTGGTCTAGCCTCATACCCAATTGACACAGTTAGGAGAAGAATGATGATGACATCAGGAGGAGCTGTCAAGTACAAGAGTTCCTTGGATGCATTCCAACAAATCCTGAAGAACGAGGGAGCTAAGTCATTGTTCAAGGGAGCTGGTGCTAACATCCTCCGTGCCGTTGCTGGTGCTGGTGTGCTTGCTGGTTATGACAAGTTGCAGGTTCTTGTTTTCGGCAAGAAATATGGATCTGGTGGAGCTTAATTATTTTTAGTGTCTTTTAAGTCAAATTTATTGTTTTTAGATGGCGATGAAAATCATTTATGTTCCGAGAGTTTAGACTAATTGCTGCAGCAAATTTTGTTTAATAAATTTACTCGGTGGGATGCTCCCAAACTCTTTTATGTTTTGGTTGGAAAGTTGAAATTGAAAGCATGATTTATTGGAGTGGCAGGATAAGTAACTGTCACTTGGAATTTCTGTTTTTTAATGTTTTGAAATATATCTTATATTCTGTTCTATTTCCAGAATAAAATGCNAANNNGTGTGTTTACC
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02984 Ps_contig_mira-and-tgicl-ass_franssen_27900
SEQUENCE=TGAACGTGGACGGATTGAGAGGAGACATTGTATCAAATAGAGCTGCTAAAGCTTTGGCTGCTTTGAAGGGAAGAGACAAAGTGAGTGTAGAGGATATTGCTACTGTCATCCCTAACTGCTTGAGACACCGTCTTAAGGAAAGGATTCCTTTTGGAGTCGATAGACTTCGGGGTTTACTCGTCACCGGAGTAAATTTTACGAAATTATTCAGCTGAAGGTNATAGATAGATAGAA
TARGET=221,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02985 Ps_contig_mira-and-tgicl-ass_franssen_27903
SEQUENCE=TTGAACGTGGACGGATTGAGAGGAGACATTGTATCAAATAGAGCTGCTAAAGCTTTGGCTGCTTTGGAAGGGAAGAGACAAAGTGAGTGTAGAGGATATTGCTACTGTCATCCCTAACTGCTTGAGACCACCGTTTTAAGGAAAAGGGTACTTTCCCGTTTGGGAGTCGATAGACGTCGGTTTACNCGCACGAGTAATTTACGAATNTTCAGCTAGGAAGGTGATAGATAGATAG
TARGET=223,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02986 Ps_contig_mira-and-tgicl-ass_franssen_27906
SEQUENCE=TGAACGTGGACGGATTGAGAGGAGACATTGTATCAAATAGAGCTGCTAAAGCTTTGGCTGCTTTGAAGGGAAGAGACAAAGTGAGTGTAGAGGATATTGCTACTGTCACTCCCTAACTGCTTGAGACCACCGTCTTAAGGAAAAGGGTNTTCCGTTTTGGAGTCGATAGACGTTCGGGTTTACTCGTCACCGAAGTAAATTTTAACGAAATTATTCAGCTGAAGGTNATAGATAGATAGAACTT
TARGET=228,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02987 Ps_contig_mira-and-tgicl-ass_franssen_27907
SEQUENCE=TGATCAAGATCTGAAAGTGAAAAAGCAGTGGTATCAACGCAGAGTTGAACGTGGACGGATTGAGAGGAGACATTGTATCAAATAGAGCTGCTAAAGCTTTGGCTGCTTTGAAGGGAAGAGACAAAGTGAGTGTAGAGGATATTGCTACTGTCATCCCTAACTGCTTGAGACACCGTCTTAGAAAGGATCCTTTGGAGTCGATAGACTCGGGTTTACTCGTCACCGAGAAATTTTACGAAATATTCAGCTGAAGGTGATAGATAGATAGAACAGTTTCTGAGTTC
TARGET=256,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02988 Ps_contig_mira-and-tgicl-ass_franssen_27908
SEQUENCE=TTCTAACACATTCATGAAGTAAAGGCTTAGAATATCTTGTATACGAATGTTACTCATGTTTAAAGACAATAAATATCCGCCATAGGAAAGGTTACATTCTAAACAAATACCAGAACTCAGAAACTGTTCTATCTATCTATCACCTTCAGCTGAATATTTCGTAAAATTTCTCGGTGACGAGTAAACCCGAGTCTATCGACTCCAAAGGATCCTTTCTAAGACGGTGTCTCAAGCAGTTAGGGATGACAGTAGCAATATCCTCTACACTCACTTTGTCTCTTCCCTTCAAAGCAGCCAAAGCTTTAGCAGCTCTATTTGATACAATGTCTCCTCTCAATCCGTCCACGTTCAACTCTGCGCACACCCTCGAGATTTTCACTTTCAGATCTTGATCAATTTGAACTGAAGAAAGTAAACTCCTTGCTGAGGTGATTTGTTCCGTGAGCTTCTCTTGCTCGGCATTGTAAGTCTCTCGAAATTCCTTCGGGTTTTTGTCGAACCTAGCTCTCTCTTCTACAATCTTCACTCTCAGTTCAGCGTCTCTAACAGTCCCCACTTGAGCATGCATTCCAAACCTATCAAGCAGTTGTGGCCTGAGCTCCCCTTCTTCAGGATTTCCAGAACCGATTAGGATAAACCGAGCAGGATGCGAGATAGAGATACCCTCTCTCTCCACTGTGTTCCAACCTGATGCAGCAGAATCCAACAACACATCAACTAAATGATCATCCAAAAGATTAACTTCATCGACGTATAAGATTCCTCTATTAGCTTTAGCTAGTAGTCCAGGCTCAAATGCCTTGACACCCTCCGTGAGAGCTTTTTCGATATCAATTGTTCCACAAACCCTATCTTCTGTAGCTCCTAAAGGTAAATCAACCATGTTAATTTTGCTCAACACGATGGAAAGTTGCTCTCCTTTTATTACTCGGTCTCGCACTTCGATTCCCATCACTTCTGGATCTTCTGGGTCTGAATTGTAAGGATCACCAAAAACAACCTTGATTTCAGGAAGTAAATCGACCAATGACCTAACCGTTGTGGACTTTCCTGTTCCTCTATCCCCCATTATCATTACACCTCCAATCTTAGGATCGATGACATTAAGGAGAAGGCAAAGCTTCATCTCATCCTGTCCAACTATAGCAGCAAAAGGATACACTGGCCTCTGGCTTTCTTTAGCAATAGTATTCAGACCCTGTTCTACAGAGTTAACTTCGGTAGCAACATTGACAACAGGAAACCGAGACTTCCCCTTTGTGCCATTAATTCCAATTCCTCCACAAAACTTTCTACCACAGCTTTGCCCTGAGGATAGAGAGAGTGTGTGAATGGAAGGGTTGGAAGAAGGGGAAGAAAGGTAGCGTGAAGGAAGAAACGCAATTGAAGAAGTGCCTAGTGTAGTTGAAGCCATAGGTGAAGGTGAAGAAACACAGTTTTTTTGTGCTAAGATTAAGGTTAGTGTTTTATTCACTTGTCATGGCAGCTGGCATGCTTTCTATTGGTTTCCAACCATCAGGATTAAGAAGATAGATTCTCTCTCCTTTTYGGTNAGATGGATAGTAAAGGTGTAGTTCACAAATATGTGAGTGTGTGTTT
TARGET=128,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02989 Ps_contig_mira-and-tgicl-ass_franssen_27909
SEQUENCE=AGTAAAGGCTTAGAATATCTTGTATACGAATGTTACTCATGTTTAAAGACAATAAATATCCGCCATAGGAAAGGTTACATTCTAAACAAATACCAGAACTCAGAAACTGTTCTATCTATCTATCACCTTCAGCTGAATATTTCGTAAAATTTCTCGGTGACGAGTAAACCCGAGTCTATCGACTCCAAAGGATCCTTTCTAAGACGGTGTCTCAAGCAGTTAGGGATGACAGTAGCAATATCCTCTACACTCACTTTGTCTCTTCCCTTCAAAGCAGCCAAAGCTTTAGCAGCTTCTTATTTGATACAATGTCTCCTCTCAAATACCGTACCAACGTTACA
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1156
PRIMER_LEFT_PENALTY=0.063454
PRIMER_RIGHT_PENALTY=0.052187
PRIMER_LEFT_SEQUENCE=AAATATCCGCCATAGGAAAGGT
PRIMER_RIGHT_SEQUENCE=TGCTTGAGACACCGTCTTAGAA
PRIMER_LEFT=53,22
PRIMER_RIGHT=217,22
PRIMER_LEFT_TM=60.063
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=0.1156
PRIMER_LEFT_1_PENALTY=0.063454
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=AATATCCGCCATAGGAAAGGTT
PRIMER_RIGHT_1_SEQUENCE=TGCTTGAGACACCGTCTTAGAA
PRIMER_LEFT_1=54,22
PRIMER_RIGHT_1=217,22
PRIMER_LEFT_1_TM=60.063
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.1881
PRIMER_LEFT_2_PENALTY=0.063454
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=AAATATCCGCCATAGGAAAGGT
PRIMER_RIGHT_2_SEQUENCE=TCATCCCTAACTGCTTGAGACA
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=228,22
PRIMER_LEFT_2_TM=60.063
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=176
PRIMER_PAIR_PENALTY_3=0.1881
PRIMER_LEFT_3_PENALTY=0.063454
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=AAATATCCGCCATAGGAAAGGT
PRIMER_RIGHT_3_SEQUENCE=TGTCATCCCTAACTGCTTGAGA
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=230,22
PRIMER_LEFT_3_TM=60.063
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=178
PRIMER_PAIR_PENALTY_4=0.1881
PRIMER_LEFT_4_PENALTY=0.063454
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=AATATCCGCCATAGGAAAGGTT
PRIMER_RIGHT_4_SEQUENCE=TCATCCCTAACTGCTTGAGACA
PRIMER_LEFT_4=54,22
PRIMER_RIGHT_4=228,22
PRIMER_LEFT_4_TM=60.063
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_02990 Ps_contig_mira-and-tgicl-ass_franssen_27920
SEQUENCE=GTCGGNGTGATTCTCTTCTTCTTCTTCATCTTCATCTTCTTCTTCTTCTACATTTCTTCATTCACATTCCAAAAAACTTCTTCTGAAGGTCAAAACGGTCATTTCCTAGATCCCTCGTTAAGCTCCACTTTCCATCGCCATGGAGCTTAGCTTCCATTGCAGAAGATCAACAACAACAACAACAACAACAACACTTGCTGTTGTTTCTCTTTCCTTGTTGATTCTCTTCTCCACTTCTAGTTTCGTTCGTGCATCTTCTTCTATCTACTCGGAGTTGAATCCTATAAAACCGAGACACTCACGCCTTCTCAAGAGCGCTGTGCAACGGGAAACTCCGACATCGCAGCTTTCTGAAATATGGACGCCTCTGGAAAATCAAGGTTGGAAACCTTGCAATGAATTGAGAAACAAGCCAACATTACCGGAGAAGTCTGAAGGATACATCCAGGTATTTCTTGATGGAGGTCTGAACCAGCAAAGGATGGGGATATGTGATGCAGTTGCTGTTGCCAAGATACTGAATGCAACTCTTGTGATCCCATACCTTGAATTAAATCCTGTATGGAAGGATTCAAGCTCATTCGAGGATATATTTGACGTGGATCATTTCATTGATGTATTGAAGGATGATGTTACTATCGTTAAAGAGCTTCCTGAAGAGTATGCATGGAGTTCAAGGGAGTACTATGCATTGGCTATTAGAGATACCAGAATCAAGGCTGCACCTGTTCATGCAACAGCCAACTGGTATCTGGAGAATGTTTTGCCGGTCCTACAAAGTTATGGTATTGCTGCAATCTCTCCGTTTTCTCACCGCCTGACTTTTGACAACTTGCCTATGGATATCCAACACCTGCGCTGTAAAGTCAACTTTCAAGCTTTAGTATTTGTTCCCCATATCAGGACACTCGGAGATGCCCTTATCAGTCGCCTTCGAAATCCTCAGCACTCTACTGATGAAATGGGCTCCAACTACCTTCAAGAGGTCACAGATGCAGATGATAGTAAAAATGCAGGGAAATTCGTTGTTCTGCACCTCCGATTTGACAAGGATATGGCAGCCCATTCAGCCTGTGATTTTGGTGGTGGGAAAGCTGAAAAATTGGCCCTTGCAAAGTATAGACAGGTTATTTGGCAGGGAAGGGTTCTTAATTCCCAATTCACTGATGAAGAGTTGAGGAGTCAGGGCCGTTGCCCAATGACTCCTGAAGAGGTTGGATTGTTGCTAGCAGCTTTGGGATTCGACAACAGCACTCGTTTATATCTTGCCTCTCACAAGGTCTATGGTGGAGGAGCCAGGATTGCTACTTTGAAGCAATTGTTTCCACTGATGGAAGATAAAAAGAGCCTTACTTCACCTTTTGAGCGTGCTCAGATAAAGGGAAAAGCTTCTCTATTAGCCGCACTTGACTACTATATCAGTATCCATAGTGACATCTTCATTTCTGCTTCACCAGGAAATATGCATAATGCATTGGTTGGACACAGGACTTACTTGAACTTAAAGACTATAAGGCCAAACATGGCATTGATGGGTCAACTGTTCTTGAATAAAACGATGGAGTGGTCAGAGTTTGAGCATGCAGTGGTTGAAGGTCATGAAAATAGAGGATATCAACGCAGAGTTAGAAAGCCTAAACAATCCATATACACATACCCTGTTCCTGATTGCATGTGCCAAGCTTAATAGATTGCTTACAGATTAGTAGCCTCTATATTTTATGATTCAGTCTTTTTATTTGAGATTTGAATCTGGTTAATAAAAGCATTATCACTAGTCCTATTCATGAGGGGTTTGTCCATGATGTTCTTGGTAAGAGTGCTTGATATAATAGTATAGTAGTAGGTGGTCTTGTGTGAGAGATGTATGTATGAGATGATGGCATTCTTAGCAGGAGCAATATTATTTATAATTCAATAACAGGAGCTATTTTGTTGTTTAT
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02991 Ps_contig_mira-and-tgicl-ass_franssen_27920
SEQUENCE=GTCGGNGTGATTCTCTTCTTCTTCTTCATCTTCATCTTCTTCTTCTTCTACATTTCTTCATTCACATTCCAAAAAACTTCTTCTGAAGGTCAAAACGGTCATTTCCTAGATCCCTCGTTAAGCTCCACTTTCCATCGCCATGGAGCTTAGCTTCCATTGCAGAAGATCAACAACAACAACAACAACAACAACACTTGCTGTTGTTTCTCTTTCCTTGTTGATTCTCTTCTCCACTTCTAGTTTCGTTCGTGCATCTTCTTCTATCTACTCGGAGTTGAATCCTATAAAACCGAGACACTCACGCCTTCTCAAGAGCGCTGTGCAACGGGAAACTCCGACATCGCAGCTTTCTGAAATATGGACGCCTCTGGAAAATCAAGGTTGGAAACCTTGCAATGAATTGAGAAACAAGCCAACATTACCGGAGAAGTCTGAAGGATACATCCAGGTATTTCTTGATGGAGGTCTGAACCAGCAAAGGATGGGGATATGTGATGCAGTTGCTGTTGCCAAGATACTGAATGCAACTCTTGTGATCCCATACCTTGAATTAAATCCTGTATGGAAGGATTCAAGCTCATTCGAGGATATATTTGACGTGGATCATTTCATTGATGTATTGAAGGATGATGTTACTATCGTTAAAGAGCTTCCTGAAGAGTATGCATGGAGTTCAAGGGAGTACTATGCATTGGCTATTAGAGATACCAGAATCAAGGCTGCACCTGTTCATGCAACAGCCAACTGGTATCTGGAGAATGTTTTGCCGGTCCTACAAAGTTATGGTATTGCTGCAATCTCTCCGTTTTCTCACCGCCTGACTTTTGACAACTTGCCTATGGATATCCAACACCTGCGCTGTAAAGTCAACTTTCAAGCTTTAGTATTTGTTCCCCATATCAGGACACTCGGAGATGCCCTTATCAGTCGCCTTCGAAATCCTCAGCACTCTACTGATGAAATGGGCTCCAACTACCTTCAAGAGGTCACAGATGCAGATGATAGTAAAAATGCAGGGAAATTCGTTGTTCTGCACCTCCGATTTGACAAGGATATGGCAGCCCATTCAGCCTGTGATTTTGGTGGTGGGAAAGCTGAAAAATTGGCCCTTGCAAAGTATAGACAGGTTATTTGGCAGGGAAGGGTTCTTAATTCCCAATTCACTGATGAAGAGTTGAGGAGTCAGGGCCGTTGCCCAATGACTCCTGAAGAGGTTGGATTGTTGCTAGCAGCTTTGGGATTCGACAACAGCACTCGTTTATATCTTGCCTCTCACAAGGTCTATGGTGGAGGAGCCAGGATTGCTACTTTGAAGCAATTGTTTCCACTGATGGAAGATAAAAAGAGCCTTACTTCACCTTTTGAGCGTGCTCAGATAAAGGGAAAAGCTTCTCTATTAGCCGCACTTGACTACTATATCAGTATCCATAGTGACATCTTCATTTCTGCTTCACCAGGAAATATGCATAATGCATTGGTTGGACACAGGACTTACTTGAACTTAAAGACTATAAGGCCAAACATGGCATTGATGGGTCAACTGTTCTTGAATAAAACGATGGAGTGGTCAGAGTTTGAGCATGCAGTGGTTGAAGGTCATGAAAATAGAGGATATCAACGCAGAGTTAGAAAGCCTAAACAATCCATATACACATACCCTGTTCCTGATTGCATGTGCCAAGCTTAATAGATTGCTTACAGATTAGTAGCCTCTATATTTTATGATTCAGTCTTTTTATTTGAGATTTGAATCTGGTTAATAAAAGCATTATCACTAGTCCTATTCATGAGGGGTTTGTCCATGATGTTCTTGGTAAGAGTGCTTGATATAATAGTATAGTAGTAGGTGGTCTTGTGTGAGAGATGTATGTATGAGATGATGGCATTCTTAGCAGGAGCAATATTATTTATAATTCAATAACAGGAGCTATTTTGTTGTTTAT
TARGET=35,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2534
PRIMER_LEFT_PENALTY=0.234977
PRIMER_RIGHT_PENALTY=0.018437
PRIMER_LEFT_SEQUENCE=TCGGNGTGATTCTCTTCTTCTT
PRIMER_RIGHT_SEQUENCE=TGATCTTCTGCAATGGAAGCTA
PRIMER_LEFT=1,22
PRIMER_RIGHT=168,22
PRIMER_LEFT_TM=59.765
PRIMER_RIGHT_TM=59.982
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.2534
PRIMER_LEFT_1_PENALTY=0.234977
PRIMER_RIGHT_1_PENALTY=0.018437
PRIMER_LEFT_1_SEQUENCE=CGGNGTGATTCTCTTCTTCTTC
PRIMER_RIGHT_1_SEQUENCE=TGATCTTCTGCAATGGAAGCTA
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=168,22
PRIMER_LEFT_1_TM=59.765
PRIMER_RIGHT_1_TM=59.982
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.3528
PRIMER_LEFT_2_PENALTY=0.234977
PRIMER_RIGHT_2_PENALTY=0.117817
PRIMER_LEFT_2_SEQUENCE=TCGGNGTGATTCTCTTCTTCTT
PRIMER_RIGHT_2_SEQUENCE=GTTGTTGATCTTCTGCAATGGA
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=173,22
PRIMER_LEFT_2_TM=59.765
PRIMER_RIGHT_2_TM=60.118
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.3528
PRIMER_LEFT_3_PENALTY=0.234977
PRIMER_RIGHT_3_PENALTY=0.117817
PRIMER_LEFT_3_SEQUENCE=CGGNGTGATTCTCTTCTTCTTC
PRIMER_RIGHT_3_SEQUENCE=GTTGTTGATCTTCTGCAATGGA
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=173,22
PRIMER_LEFT_3_TM=59.765
PRIMER_RIGHT_3_TM=60.118
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.3636
PRIMER_LEFT_4_PENALTY=0.234977
PRIMER_RIGHT_4_PENALTY=0.128591
PRIMER_LEFT_4_SEQUENCE=CGGNGTGATTCTCTTCTTCTTC
PRIMER_RIGHT_4_SEQUENCE=CTAGGAAATGACCGTTTTGACC
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=108,22
PRIMER_LEFT_4_TM=59.765
PRIMER_RIGHT_4_TM=59.871
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=107
=
PRIMER_SEQUENCE_ID=SSR_02992 Ps_contig_mira-and-tgicl-ass_franssen_27920
SEQUENCE=GTCGGNGTGATTCTCTTCTTCTTCTTCATCTTCATCTTCTTCTTCTTCTACATTTCTTCATTCACATTCCAAAAAACTTCTTCTGAAGGTCAAAACGGTCATTTCCTAGATCCCTCGTTAAGCTCCACTTTCCATCGCCATGGAGCTTAGCTTCCATTGCAGAAGATCAACAACAACAACAACAACAACAACACTTGCTGTTGTTTCTCTTTCCTTGTTGATTCTCTTCTCCACTTCTAGTTTCGTTCGTGCATCTTCTTCTATCTACTCGGAGTTGAATCCTATAAAACCGAGACACTCACGCCTTCTCAAGAGCGCTGTGCAACGGGAAACTCCGACATCGCAGCTTTCTGAAATATGGACGCCTCTGGAAAATCAAGGTTGGAAACCTTGCAATGAATTGAGAAACAAGCCAACATTACCGGAGAAGTCTGAAGGATACATCCAGGTATTTCTTGATGGAGGTCTGAACCAGCAAAGGATGGGGATATGTGATGCAGTTGCTGTTGCCAAGATACTGAATGCAACTCTTGTGATCCCATACCTTGAATTAAATCCTGTATGGAAGGATTCAAGCTCATTCGAGGATATATTTGACGTGGATCATTTCATTGATGTATTGAAGGATGATGTTACTATCGTTAAAGAGCTTCCTGAAGAGTATGCATGGAGTTCAAGGGAGTACTATGCATTGGCTATTAGAGATACCAGAATCAAGGCTGCACCTGTTCATGCAACAGCCAACTGGTATCTGGAGAATGTTTTGCCGGTCCTACAAAGTTATGGTATTGCTGCAATCTCTCCGTTTTCTCACCGCCTGACTTTTGACAACTTGCCTATGGATATCCAACACCTGCGCTGTAAAGTCAACTTTCAAGCTTTAGTATTTGTTCCCCATATCAGGACACTCGGAGATGCCCTTATCAGTCGCCTTCGAAATCCTCAGCACTCTACTGATGAAATGGGCTCCAACTACCTTCAAGAGGTCACAGATGCAGATGATAGTAAAAATGCAGGGAAATTCGTTGTTCTGCACCTCCGATTTGACAAGGATATGGCAGCCCATTCAGCCTGTGATTTTGGTGGTGGGAAAGCTGAAAAATTGGCCCTTGCAAAGTATAGACAGGTTATTTGGCAGGGAAGGGTTCTTAATTCCCAATTCACTGATGAAGAGTTGAGGAGTCAGGGCCGTTGCCCAATGACTCCTGAAGAGGTTGGATTGTTGCTAGCAGCTTTGGGATTCGACAACAGCACTCGTTTATATCTTGCCTCTCACAAGGTCTATGGTGGAGGAGCCAGGATTGCTACTTTGAAGCAATTGTTTCCACTGATGGAAGATAAAAAGAGCCTTACTTCACCTTTTGAGCGTGCTCAGATAAAGGGAAAAGCTTCTCTATTAGCCGCACTTGACTACTATATCAGTATCCATAGTGACATCTTCATTTCTGCTTCACCAGGAAATATGCATAATGCATTGGTTGGACACAGGACTTACTTGAACTTAAAGACTATAAGGCCAAACATGGCATTGATGGGTCAACTGTTCTTGAATAAAACGATGGAGTGGTCAGAGTTTGAGCATGCAGTGGTTGAAGGTCATGAAAATAGAGGATATCAACGCAGAGTTAGAAAGCCTAAACAATCCATATACACATACCCTGTTCCTGATTGCATGTGCCAAGCTTAATAGATTGCTTACAGATTAGTAGCCTCTATATTTTATGATTCAGTCTTTTTATTTGAGATTTGAATCTGGTTAATAAAAGCATTATCACTAGTCCTATTCATGAGGGGTTTGTCCATGATGTTCTTGGTAAGAGTGCTTGATATAATAGTATAGTAGTAGGTGGTCTTGTGTGAGAGATGTATGTATGAGATGATGGCATTCTTAGCAGGAGCAATATTATTTATAATTCAATAACAGGAGCTATTTTGTTGTTTAT
TARGET=168,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1464
PRIMER_LEFT_PENALTY=0.128591
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=AGGTCAAAACGGTCATTTCCTA
PRIMER_RIGHT_SEQUENCE=TAGAAGAAGATGCACGAACGAA
PRIMER_LEFT=86,22
PRIMER_RIGHT=262,22
PRIMER_LEFT_TM=59.871
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.1464
PRIMER_LEFT_1_PENALTY=0.128591
PRIMER_RIGHT_1_PENALTY=0.017846
PRIMER_LEFT_1_SEQUENCE=CTAGATCCCTCGTTAAGCTCCA
PRIMER_RIGHT_1_SEQUENCE=TAGAAGAAGATGCACGAACGAA
PRIMER_LEFT_1=105,22
PRIMER_RIGHT_1=262,22
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=0.1618
PRIMER_LEFT_2_PENALTY=0.143968
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=TTCTTCTGAAGGTCAAAACGGT
PRIMER_RIGHT_2_SEQUENCE=TAGAAGAAGATGCACGAACGAA
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=60.144
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.3000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=0.1870
PRIMER_LEFT_3_PENALTY=0.169174
PRIMER_RIGHT_3_PENALTY=0.017846
PRIMER_LEFT_3_SEQUENCE=AGCTTAGCTTCCATTGCAGAAG
PRIMER_RIGHT_3_SEQUENCE=TAGAAGAAGATGCACGAACGAA
PRIMER_LEFT_3=143,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=60.169
PRIMER_RIGHT_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=0.2204
PRIMER_LEFT_4_PENALTY=0.202525
PRIMER_RIGHT_4_PENALTY=0.017846
PRIMER_LEFT_4_SEQUENCE=AACGGTCATTTCCTAGATCCCT
PRIMER_RIGHT_4_SEQUENCE=TAGAAGAAGATGCACGAACGAA
PRIMER_LEFT_4=93,22
PRIMER_RIGHT_4=262,22
PRIMER_LEFT_4_TM=60.203
PRIMER_RIGHT_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_02993 Ps_contig_mira-and-tgicl-ass_franssen_27981
SEQUENCE=AAGACNCTTGAATGGAGCCAGCTTTTTTCTAATCAGCCAAACCTACACAAGTGAAGAGNGTAAAATAGCTTGRTAGGCAGGTGTTTAGTATGAGCCTCTTCTTTGTAGGCTCACAATTTTCTGAAAGTGAGAATGATAAATAATGAGCATATACACGGAACAGCCGCTTGAATATTGTCTTCACGACATCCTTAAAGTTGGGAGGAAACGGAGAACCAAGCTTCTGAGGGAATATGGATTCATCATCAAGCTGTGTTTCAACCCAGTCCATCAGATATTCTACATATTTTGGAGCAGAAACCTCAATAGGTTTCTTAATTTGCACACCATCTGCCCATCTATATTCATACTTGGGTCCTGCAGACATTGTGCGACAATTCTCGGGAGTACAGAACTCTGTGAGGGTGCCATAGAGCAGATTCACCTGGTTGAAAAAATCAACAGTGTTGACAGCTAGCCACTCATTCAAATCCTCTCCAGGAGGTAGCTTTACTGCTTCCCTCAGATTTCCACTACCTAATGTTGCATCAATGTGTTTTCTAAGCTGAGCTCCCTTGCTTCCAGAAGGAGTACTTTTTTTTGGGCGGAATGTTCTCTGATTTCTGCCAATACCGAAGAGGCTCATGACTTGGGTGGGAAGAAGAAGAAGAAGAGGAAGAGAGAAAGTGCGTTGAGTCGAAGCCCTCTCTTTCTTCCTCGGTTTCATTTTTTTCAGGCGTTAGTTCTACTCTTTCCTACTTTAGATTTTCCCCCCGAATTATTTTGTGT
TARGET=637,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_02994 Ps_contig_mira-and-tgicl-ass_franssen_28025
SEQUENCE=CTTAAATGTAACATGCAGCAATTACCTTATTGGTAATTAAAAATTTTCATTTATCATCATCCGTTGGGAATAGCATGTAAATAACCAGAAAGTTTTCACCAAATGTCTCATGAATATTCACAAATGACAATTAATTTCCCTTGCAATGGTTGAGTTCCATTGACAACAATAACAAAAGCAATCATTCAAAATATCTTTGGTTTACATAAAGCATGAGTGTGCGGTTGCATTAAACAGTTGAGAGGATGATTTGGTCATCCCATGGCATACTTCTGTGTCCAGCTGCGTGCGGTGGCTTCGTACTTGGTCCTGTCGGTCTTGTACATATGTGCGATTTCAGGCACCAAAGGGTCGTCGGGATTGGGATCCGTCAGCAACGAGCAAATCGACAAGAGGACCTTGGAAATGGTCAACGCTGGGCTCCATTGTTCTTTCAGAATGTCGAGACAGATACTTCCGTTGCTGTTGATGTTTGGATGGAATACCTTAGTCCTAAATGCAACCTTTGGTGGCTTGAATGGATAATCGGGAGGAAAGTGAATTGAAACCAAAAACACACCACCTGAATAGGGGCTATCAGCAGGTCCCATGATTGTTGCTTGCCAGTGAAACATGTCCTCAGCAACAGGACCTGCGCTGCATGAGGTAGGAGGATCCTTCTGCAAATCCTTGAGTTCCTTGAGGATGCGTTTGGATGCCATGCTGAAAACCAAGGATTCGGAGGAAGAAGATGAGAATTCGGGGAGAAGGTTGAAATTGAAAAGGGTTTGATCGAAGAAGAATTGGATTTGATTTGAGTCAGGGGAAAGGGATAATAAGGTGAGAGAGAGAAAAAGGGACAGACAGGGTTGCGTTGTTTTTCTGCTTCCAGAATGTTCTGTATCCACTTTCCTATTTGCGTGTGTTTGGGGCCGCGTTTTTGACTCAAAAACGCC
TARGET=822,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1566
PRIMER_LEFT_PENALTY=0.054900
PRIMER_RIGHT_PENALTY=0.101680
PRIMER_LEFT_SEQUENCE=GGTTTGATCGAAGAAGAATTGG
PRIMER_RIGHT_SEQUENCE=ACACGCAAATAGGAAAGTGGAT
PRIMER_LEFT=764,22
PRIMER_RIGHT=902,22
PRIMER_LEFT_TM=59.945
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=139
PRIMER_PAIR_PENALTY_1=0.1566
PRIMER_LEFT_1_PENALTY=0.054900
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=GGGTTTGATCGAAGAAGAATTG
PRIMER_RIGHT_1_SEQUENCE=ACACGCAAATAGGAAAGTGGAT
PRIMER_LEFT_1=763,22
PRIMER_RIGHT_1=902,22
PRIMER_LEFT_1_TM=59.945
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=140
PRIMER_PAIR_PENALTY_2=0.3094
PRIMER_LEFT_2_PENALTY=0.207756
PRIMER_RIGHT_2_PENALTY=0.101680
PRIMER_LEFT_2_SEQUENCE=GATCCTTCTGCAAATCCTTGAG
PRIMER_RIGHT_2_SEQUENCE=ACACGCAAATAGGAAAGTGGAT
PRIMER_LEFT_2=652,22
PRIMER_RIGHT_2=902,22
PRIMER_LEFT_2_TM=60.208
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.3130
PRIMER_LEFT_3_PENALTY=0.211336
PRIMER_RIGHT_3_PENALTY=0.101680
PRIMER_LEFT_3_SEQUENCE=GATTCGGAGGAAGAAGATGAGA
PRIMER_RIGHT_3_SEQUENCE=ACACGCAAATAGGAAAGTGGAT
PRIMER_LEFT_3=714,22
PRIMER_RIGHT_3=902,22
PRIMER_LEFT_3_TM=59.789
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.3432
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=0.101680
PRIMER_LEFT_4_SEQUENCE=TCTGCAAATCCTTGAGTTCCTT
PRIMER_RIGHT_4_SEQUENCE=ACACGCAAATAGGAAAGTGGAT
PRIMER_LEFT_4=658,22
PRIMER_RIGHT_4=902,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_02995 Ps_contig_mira-and-tgicl-ass_franssen_28092
SEQUENCE=CCTTCGTCGGACGTTCCTTCCTTCCTTTTCCTTCCTAGGACTAAAAGTTACATTCTTAATGTTAAAAGTTTTAGTTCGGAGTTCCTTCCTGGTTGGTCTTTCCCTTTCGTATCATGTCCGCTACAGTCTGGCATGTGAGATCAAGAAGGCTCTTGATGTTCAAATAGTTTGCGGCCAGAATGAGATCGAACAGCGTTGCCTGGTCAACCTTGACGAACTCTGCATCCCAAGCCTTGAGTTCATCTTCAGTAGGGTTTTTCATCAGAACTCACCGCATCAACGTTGTTTTCTTACAGTATTCAATCTACTTTAGACAAGGTATCTTGCTCGTAACGTGGAAGA
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_02996 Ps_contig_mira-and-tgicl-ass_franssen_28103
SEQUENCE=GTCGGGGNAACTCAAAACAAATCAACACAATCCCTAATATCTCTCTCTCCATTTTTACCAAATCAATTTGCAATGGCTCGTACTAAGCAAACAGCACGCAAATCCACCGGAGGCAAAGCACCAAGGAAGCAGCTAGCAACAAAAGCTGCTCGCAAATCAGCACCGGCAACCGGAGGAGTGAAGAAGCCACACAGATTCAGGCCAGGAACTGTTGCTCTTAGAGAAATCAGGAAGTACCAGAAGAGCACTGAGCTTCTCATAAGGAAGCTTCCATTCCAGAGGCTAGTGAGGGAAATCGCTCAGGATTTCAAAACTGATCTCCGATTCCAAAGCAGCGCTGTTTCTGCTCTACAAGAAGCAGCTGAAGCTTACCTTGTTGGTTTGTTCGAAGACACTAACCTCTGCGCCATTCATGCTAAGAGAGTTACCATTATGCCCAAGGATATTCAGCTTGCTCGCAGAATCAGAGGAGAGAGAGCTTAAATTCCATTACTATGGTTCAATTTCTTTAGGGGTTGTATGTGTTTCTTAATTAGGGTTAGGTTTATGATGTTGTTGTTACTTTATATTCTGTTTAAATTTATCTTGTTATCATCTTGTAACAATGAAATTTGAGAATTCAAACTGTCATAGTGATGCGTTGAAACACAATCTGTGCGTTCCCCTGTTTATTAATCAATTTCCG
TARGET=40,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6396
PRIMER_LEFT_PENALTY=1.437791
PRIMER_RIGHT_PENALTY=0.201815
PRIMER_LEFT_SEQUENCE=GGGGNAACTCAAAACAAATCA
PRIMER_RIGHT_SEQUENCE=CTTTTGTTGCTAGCTGCTTCCT
PRIMER_LEFT=3,21
PRIMER_RIGHT=144,22
PRIMER_LEFT_TM=59.562
PRIMER_RIGHT_TM=60.202
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=142
PRIMER_PAIR_PENALTY_1=1.7367
PRIMER_LEFT_1_PENALTY=1.437791
PRIMER_RIGHT_1_PENALTY=0.298898
PRIMER_LEFT_1_SEQUENCE=GGGGNAACTCAAAACAAATCA
PRIMER_RIGHT_1_SEQUENCE=TGAATCTGTGTGGCTTCTTCAC
PRIMER_LEFT_1=3,21
PRIMER_RIGHT_1=198,22
PRIMER_LEFT_1_TM=59.562
PRIMER_RIGHT_1_TM=60.299
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=1.7726
PRIMER_LEFT_2_PENALTY=1.437791
PRIMER_RIGHT_2_PENALTY=0.334827
PRIMER_LEFT_2_SEQUENCE=GGGGNAACTCAAAACAAATCA
PRIMER_RIGHT_2_SEQUENCE=AGCTTTTGTTGCTAGCTGCTTC
PRIMER_LEFT_2=3,21
PRIMER_RIGHT_2=146,22
PRIMER_LEFT_2_TM=59.562
PRIMER_RIGHT_2_TM=60.335
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=1.8693
PRIMER_LEFT_3_PENALTY=1.437791
PRIMER_RIGHT_3_PENALTY=0.431486
PRIMER_LEFT_3_SEQUENCE=GGGGNAACTCAAAACAAATCA
PRIMER_RIGHT_3_SEQUENCE=CTGAATCTGTGTGGCTTCTTCA
PRIMER_LEFT_3=3,21
PRIMER_RIGHT_3=199,22
PRIMER_LEFT_3_TM=59.562
PRIMER_RIGHT_3_TM=60.431
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=1.9760
PRIMER_LEFT_4_PENALTY=1.437791
PRIMER_RIGHT_4_PENALTY=0.538176
PRIMER_LEFT_4_SEQUENCE=GGGGNAACTCAAAACAAATCA
PRIMER_RIGHT_4_SEQUENCE=TTTTGTTGCTAGCTGCTTCCTT
PRIMER_LEFT_4=3,21
PRIMER_RIGHT_4=143,22
PRIMER_LEFT_4_TM=59.562
PRIMER_RIGHT_4_TM=60.538
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_02997 Ps_contig_mira-and-tgicl-ass_franssen_28105
SEQUENCE=TAGATATGTTTCTAAACCGTCTTAGAAATGAAAACAAACAACACAACTAATATCAAAATAATAATATCTAGTAAACCGCATTTTGATTAATTGTTTTTCTCTGTTACATTGCAAATTGAACAGAAAAACAAATTACTCAAATACAATTAAGAGAAACAGACTTTATCAACTTTCAAGATTCAAAAACTGGTGCCTAGCCGTAACAGTGCGTCATCTACAGAATATGATTCCCTTCAATCAAATCTAAGCCCTCTCTCCTCTGATTCTACGAGCAAGTTGAATATCCTTAGGCATAATGGTAACTCTCTTAGCGTGAATTGCACAAAGGTTAGTATCTTCAAACAAACCAACTAGATATGCTTCAGCAGCTTCTTGAAGTGCAGAAACAGCACTGCTTTGGAATCGGAGATCTGTTTTGAAATCTTGAGCGATTTCTCTGACTAATCTTTGGAATGGAAGCTTCCTGATGAGAAGCTCTGTGCTCTTCTGATACTTCCTGATCTCTCTCAAAGCAACTGTTCCTGGACGGAATCTGTGTGGCTTCTTCACTCCTCCGGTCGCCGGAGCAGATTTACGAGCGGCTTTTGTTGCCAACTGTTTCCTTGGAGCTTTACCTCCGGTGGATTTGCGCGCGGTTTGTTTGGTACGAGCCATTTCGATCGGAACGAAAACAGCGAGAAAAATAAAATTGAGAGAGAGAGAGAAGAAGGAAAATATGCGATCCC
TARGET=691,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.6135
PRIMER_LEFT_PENALTY=0.073355
PRIMER_RIGHT_PENALTY=3.540185
PRIMER_LEFT_SEQUENCE=TGAGAAGCTCTGTGCTCTTCTG
PRIMER_RIGHT_SEQUENCE=GGGATCGCATATTTTCCTTC
PRIMER_LEFT=467,22
PRIMER_RIGHT=724,20
PRIMER_LEFT_TM=60.073
PRIMER_RIGHT_TM=58.460
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=3.7008
PRIMER_LEFT_1_PENALTY=0.160609
PRIMER_RIGHT_1_PENALTY=3.540185
PRIMER_LEFT_1_SEQUENCE=GCTTCCTGATGAGAAGCTCTGT
PRIMER_RIGHT_1_SEQUENCE=GGGATCGCATATTTTCCTTC
PRIMER_LEFT_1=458,22
PRIMER_RIGHT_1=724,20
PRIMER_LEFT_1_TM=60.161
PRIMER_RIGHT_1_TM=58.460
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=4.0846
PRIMER_LEFT_2_PENALTY=0.544368
PRIMER_RIGHT_2_PENALTY=3.540185
PRIMER_LEFT_2_SEQUENCE=CTTTTGTTGCCAACTGTTTCCT
PRIMER_RIGHT_2_SEQUENCE=GGGATCGCATATTTTCCTTC
PRIMER_LEFT_2=581,22
PRIMER_RIGHT_2=724,20
PRIMER_LEFT_2_TM=60.544
PRIMER_RIGHT_2_TM=58.460
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=4.1588
PRIMER_LEFT_3_PENALTY=0.618623
PRIMER_RIGHT_3_PENALTY=3.540185
PRIMER_LEFT_3_SEQUENCE=TGATGAGAAGCTCTGTGCTCTT
PRIMER_RIGHT_3_SEQUENCE=GGGATCGCATATTTTCCTTC
PRIMER_LEFT_3=464,22
PRIMER_RIGHT_3=724,20
PRIMER_LEFT_3_TM=59.381
PRIMER_RIGHT_3_TM=58.460
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=4.2305
PRIMER_LEFT_4_PENALTY=0.690327
PRIMER_RIGHT_4_PENALTY=3.540185
PRIMER_LEFT_4_SEQUENCE=ATCTGTGTGGCTTCTTCACTCC
PRIMER_RIGHT_4_SEQUENCE=GGGATCGCATATTTTCCTTC
PRIMER_LEFT_4=530,22
PRIMER_RIGHT_4=724,20
PRIMER_LEFT_4_TM=60.690
PRIMER_RIGHT_4_TM=58.460
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_02998 Ps_contig_mira-and-tgicl-ass_franssen_28138
SEQUENCE=AACAAAGAGTTATNTCAACAAAACNCATAAACATAGAAAGACAATTAGGAATTAGATTGACTGGTTTAGACTGAATATGTATCCGCTAAATTAGAGGTGCTTAAACTAAACTATCAACACAGAACAAAGACCTAGAATCTAAGGCCATCTTTCTAGTCTAAGCTTTCTTAGGAGTTTTCTTGGCCTTTGGAGATTTAGGCTCCTTTGCAGAAGAAGAAGCATTTTCAGTCCTCTTAGGCNAAAGGACAGGGTTGATGTTAGGTAGAACACCACCATGAGCAATGGTAACACCAGCCAGCAATTTTCCAAGCTCTTCATCATTCCTTACTGCCAACAACAAATGTCTTGGACTGATTCTATTCTTCTTGTTGTCACGGGCAGCATTCCCAGCCAACTCAAGAACCTCAGCAGCAAGATATTCGAGAACTGCAGCCAAGTAAACTGGAGCACCAGTACCAACACGCTGAGCGTATCTACCTTTCTTCAAGAAACGACCGATTCTTCCGACGGGAATTGAAGACCAGCTCTGACGGATCTGGTCACTGACTTCTTCCTTGGTCCTCCTCCTTCCTTCCTCCGGCCATTTAGAACACTAGTTTTTTGCAGCTTACTCGCTGTTTTGTAAGAAAACGTGAGTAA
TARGET=565,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3286
PRIMER_LEFT_PENALTY=0.061818
PRIMER_RIGHT_PENALTY=0.266783
PRIMER_LEFT_SEQUENCE=CGCTGAGCGTATCTACCTTTCT
PRIMER_RIGHT_SEQUENCE=TACAAAACAGCGAGTAAGCTGC
PRIMER_LEFT=461,22
PRIMER_RIGHT=623,22
PRIMER_LEFT_TM=60.062
PRIMER_RIGHT_TM=59.733
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.5144
PRIMER_LEFT_1_PENALTY=0.247631
PRIMER_RIGHT_1_PENALTY=0.266783
PRIMER_LEFT_1_SEQUENCE=CTCAGCAGCAAGATATTCGAGA
PRIMER_RIGHT_1_SEQUENCE=TACAAAACAGCGAGTAAGCTGC
PRIMER_LEFT_1=403,22
PRIMER_RIGHT_1=623,22
PRIMER_LEFT_1_TM=59.752
PRIMER_RIGHT_1_TM=59.733
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.5213
PRIMER_LEFT_2_PENALTY=0.254537
PRIMER_RIGHT_2_PENALTY=0.266783
PRIMER_LEFT_2_SEQUENCE=GGAATTGAAGACCAGCTCTGAC
PRIMER_RIGHT_2_SEQUENCE=TACAAAACAGCGAGTAAGCTGC
PRIMER_LEFT_2=509,22
PRIMER_RIGHT_2=623,22
PRIMER_LEFT_2_TM=60.255
PRIMER_RIGHT_2_TM=59.733
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=115
PRIMER_PAIR_PENALTY_3=0.6125
PRIMER_LEFT_3_PENALTY=0.345718
PRIMER_RIGHT_3_PENALTY=0.266783
PRIMER_LEFT_3_SEQUENCE=CAAGAACCTCAGCAGCAAGATA
PRIMER_RIGHT_3_SEQUENCE=TACAAAACAGCGAGTAAGCTGC
PRIMER_LEFT_3=396,22
PRIMER_RIGHT_3=623,22
PRIMER_LEFT_3_TM=59.654
PRIMER_RIGHT_3_TM=59.733
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=1.0264
PRIMER_LEFT_4_PENALTY=0.759590
PRIMER_RIGHT_4_PENALTY=0.266783
PRIMER_LEFT_4_SEQUENCE=ATATTCGAGAACTGCAGCCAAG
PRIMER_RIGHT_4_SEQUENCE=TACAAAACAGCGAGTAAGCTGC
PRIMER_LEFT_4=415,22
PRIMER_RIGHT_4=623,22
PRIMER_LEFT_4_TM=60.760
PRIMER_RIGHT_4_TM=59.733
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_02999 Ps_contig_mira-and-tgicl-ass_franssen_28154
SEQUENCE=CAACGCAGAGTACGCGGGGATCGATTCTCAATTTCATACTCTACACACCAAGCTTTCTTTCAGCGTTAAAATTTCATTTTGCGACTTTCGATTAAATGGCGCCTAAGGGAGAGAAGAAGCCTGCGGAGAAGAAACCAGTGGAGGAGAAGAAATCAACTGTAGCTGAGAAGGTTCCGGCAGAGAAGAAGCCAAAGGCCGGAAAGAAGCTACCTAAGGACGGTGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGTGTAGAGACATACAAGATCTACATCTTCAAGGTTCTGAAACAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCTATGGGTATCATGAACAGTTTCATCAACGACATCTTCGAGAAGCTAGCTGCAGAATCTTCAAGACTTGCAAGGTACAACAAGAAGCCAACAATCACTTCAAGGGAAATTCAGACTGCAGTCAGACTAGTTCTTCCCGGAGAATTGGCGAAGCATGCTGTCTCCGAGGGTACAAAAGCTGTAACTAAGTTCACAAGTGCATTAAACTTTTGAAAATATTTTAAGATTTGATTGAGTTAATGAGATCATGGTGTATTTTTGTTCTTCTAAATAAGTGGTAGTTTAGTATTT
TARGET=240,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1726
PRIMER_LEFT_PENALTY=0.056850
PRIMER_RIGHT_PENALTY=0.115769
PRIMER_LEFT_SEQUENCE=AACTGTAGCTGAGAAGGTTCCG
PRIMER_RIGHT_SEQUENCE=ATACCCATAGCCTTGCTTGAGA
PRIMER_LEFT=154,22
PRIMER_RIGHT=348,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=60.116
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.1726
PRIMER_LEFT_1_PENALTY=0.056850
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=AACTGTAGCTGAGAAGGTTCCG
PRIMER_RIGHT_1_SEQUENCE=TACCCATAGCCTTGCTTGAGAT
PRIMER_LEFT_1=154,22
PRIMER_RIGHT_1=347,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=194
PRIMER_PAIR_PENALTY_2=0.1726
PRIMER_LEFT_2_PENALTY=0.056850
PRIMER_RIGHT_2_PENALTY=0.115769
PRIMER_LEFT_2_SEQUENCE=AACTGTAGCTGAGAAGGTTCCG
PRIMER_RIGHT_2_SEQUENCE=ACCCATAGCCTTGCTTGAGATA
PRIMER_LEFT_2=154,22
PRIMER_RIGHT_2=346,22
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=60.116
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=193
PRIMER_PAIR_PENALTY_3=0.1726
PRIMER_LEFT_3_PENALTY=0.056850
PRIMER_RIGHT_3_PENALTY=0.115769
PRIMER_LEFT_3_SEQUENCE=AACTGTAGCTGAGAAGGTTCCG
PRIMER_RIGHT_3_SEQUENCE=GATACCCATAGCCTTGCTTGAG
PRIMER_LEFT_3=154,22
PRIMER_RIGHT_3=349,22
PRIMER_LEFT_3_TM=59.943
PRIMER_RIGHT_3_TM=60.116
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=0.1726
PRIMER_LEFT_4_PENALTY=0.056850
PRIMER_RIGHT_4_PENALTY=0.115769
PRIMER_LEFT_4_SEQUENCE=AACTGTAGCTGAGAAGGTTCCG
PRIMER_RIGHT_4_SEQUENCE=CCATAGCCTTGCTTGAGATACC
PRIMER_LEFT_4=154,22
PRIMER_RIGHT_4=344,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=60.116
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=6.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_03000 Ps_contig_mira-and-tgicl-ass_franssen_28155
SEQUENCE=CAACGCAGAGTACGNGGGGATCGACTTCCAATTTCTGCATCCTCTTCAATTTCTACGTTGTATCCCTAAACTTTTCCAAACTAGAATTTCTAAATTCCTTCAATGGCGCCAAAGGGAGAGAAGAAACCAGTTGAGAAGAAACCTGTGGAGGAGAAGAAGTCCACAGTAGCAGAGAAAGCTCCGGCCGAGAAGAAGCCAAAGGCCGGAAAGAAGCTGCCTAAGGATGGCGGTGCCGCTGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGTATCATGAACAGTTTCATCAACGACATCTTCGAGAAGCTTGCTGCTGAATCTTCAAGACTTGTA
TARGET=247,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2618
PRIMER_LEFT_PENALTY=0.143960
PRIMER_RIGHT_PENALTY=0.117817
PRIMER_LEFT_SEQUENCE=GAGAAGAAACCTGTGGAGGAGA
PRIMER_RIGHT_SEQUENCE=TCGAAGATGTCGTTGATGAAAC
PRIMER_LEFT=132,22
PRIMER_RIGHT=385,22
PRIMER_LEFT_TM=59.856
PRIMER_RIGHT_TM=60.118
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.2636
PRIMER_LEFT_1_PENALTY=0.145769
PRIMER_RIGHT_1_PENALTY=0.117817
PRIMER_LEFT_1_SEQUENCE=GTTGAGAAGAAACCTGTGGAGG
PRIMER_RIGHT_1_SEQUENCE=TCGAAGATGTCGTTGATGAAAC
PRIMER_LEFT_1=129,22
PRIMER_RIGHT_1=385,22
PRIMER_LEFT_1_TM=60.146
PRIMER_RIGHT_1_TM=60.118
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.2686
PRIMER_LEFT_2_PENALTY=0.143960
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=GAGAAGAAACCTGTGGAGGAGA
PRIMER_RIGHT_2_SEQUENCE=CTTCTCGAAGATGTCGTTGATG
PRIMER_LEFT_2=132,22
PRIMER_RIGHT_2=389,22
PRIMER_LEFT_2_TM=59.856
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.2704
PRIMER_LEFT_3_PENALTY=0.145769
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=GTTGAGAAGAAACCTGTGGAGG
PRIMER_RIGHT_3_SEQUENCE=CTTCTCGAAGATGTCGTTGATG
PRIMER_LEFT_3=129,22
PRIMER_RIGHT_3=389,22
PRIMER_LEFT_3_TM=60.146
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.2913
PRIMER_LEFT_4_PENALTY=0.143960
PRIMER_RIGHT_4_PENALTY=0.147295
PRIMER_LEFT_4_SEQUENCE=GAGAAGAAACCTGTGGAGGAGA
PRIMER_RIGHT_4_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_4=132,22
PRIMER_RIGHT_4=339,22
PRIMER_LEFT_4_TM=59.856
PRIMER_RIGHT_4_TM=59.853
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_03001 Ps_contig_mira-and-tgicl-ass_franssen_28156
SEQUENCE=AAAAGAACAAAGTAGGTAATAYNAACGCAGAGTNCGCGGGGATAACTTCCTTCATCTGTTTATCAATTCCCAAATCGAAACCCTAACTTTTATCAAATTTCCAAGTTTCCCAATCCATTTCAATGGCGCCAAAGGGAGAGAAGAAGCCGGCGGAGAAGAAACCAGTGGAGGAGAAGAAGTCGACGGTTGCCGAGAAGACTCCCGCCGAGAAGAAGCCAAAGGCCGGGAAGAAGCTGCCTAAGGATGGCGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGAATCATGAACAGTTTCATTAACGACATCTTCGAGAAGCTTGCTGCTGAATCTTCAAGACTTGCAAGGTACAACAAGAAGCCAACAATAACTTCAAGGGAAATTCAAACTGCGGTCAGGCTTGTTCTACCTGGTGAATTGGCCAAGCATGCTGTCTCCGAGGGTACAAAAGCTGTAACCAAGTTTACCAGTGCTTAAACATGCCGTTTGTTCATTGGGTTGTTATGTCCTTGTATCTGATTTTAGTTAGGTATATTAGTTTGTTGATTCTCTTTTCCTTTAAATTGAAGTTGTAATGAAGTGGATCTAGTTTGGTCATGTGTATTAAAATTGTAGAATCAATGATAATGTTAAACTTCAAAATTGGTTCGGTAAAACTACTATTTTATTTTCTAGAAATA
TARGET=267,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03002 Ps_contig_mira-and-tgicl-ass_franssen_28157
SEQUENCE=CTAGGCTTTGAACTCTTAGTTATATTTGTCAAGTTAACAGGAGAGAGATACATACATTTAGTGATCAAATATAATTATAATCGGCACTTTAATACACAAAGGAAACAACAGATTCAATTGTTTCAGGATACAAGCCTTAACAAAGAAAGAAGCTCCTAATTGATATATTTAATAACTAAAACTGGATCCATCAAACAAAATATCACCAGCCTTAAAGAGTCTAAGAACTTGTAAACTTAGTAACAGCTTTGGTGCCCTCAGAGACGGCGTGCTTGGCTAATTCTCCGGGCAGAACAAGTCTAACCGCGGTCTGAATTTCCCTTGATGTAATTGTTGGCTTCTTGTTGTATCTTGCAAGTCTTGAAGACTCTGCAGCAAGCTTCTCGAAGATGTCGTTGATGAAACTGTTCATGATACCCATGGCCTTGCTTGAGATACCAATGTCAGGGTGAACTTGCTTCAGAACCTTGAAGATGTAGATCTTGTATGTCTCCACGCTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCGGCGGCACCACCATCCTTAGGCAGCTTCTTTCCGGCCTTTGGCTTTTTCTCAGCCGGAGTCTTCTCAGCTACCGTCGACTTCTTCTCCTCTATGGGTTTCTTCTCCGCTGGCTTCTTCTCTCCCTTTGGTGCCATTTCTGTTTGGGAATTTGAAAAACTAGAAAATTAGAAATAAGATGTAAAGATGCAGATACGAAAAACTNGTTGAACTAACCCGAAC
TARGET=511,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1116
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.081328
PRIMER_LEFT_SEQUENCE=TGAACTTGCTTCAGAACCTTGA
PRIMER_RIGHT_SEQUENCE=AGTCGACGGTAGCTGAGAAGAC
PRIMER_LEFT=449,22
PRIMER_RIGHT=611,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.1116
PRIMER_LEFT_1_PENALTY=0.030276
PRIMER_RIGHT_1_PENALTY=0.081328
PRIMER_LEFT_1_SEQUENCE=TGAACTTGCTTCAGAACCTTGA
PRIMER_RIGHT_1_SEQUENCE=GTCGACGGTAGCTGAGAAGACT
PRIMER_LEFT_1=449,22
PRIMER_RIGHT_1=610,22
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.1991
PRIMER_LEFT_2_PENALTY=0.117817
PRIMER_RIGHT_2_PENALTY=0.081328
PRIMER_LEFT_2_SEQUENCE=TCGAAGATGTCGTTGATGAAAC
PRIMER_RIGHT_2_SEQUENCE=AGTCGACGGTAGCTGAGAAGAC
PRIMER_LEFT_2=383,22
PRIMER_RIGHT_2=611,22
PRIMER_LEFT_2_TM=60.118
PRIMER_RIGHT_2_TM=60.081
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=0.1991
PRIMER_LEFT_3_PENALTY=0.117817
PRIMER_RIGHT_3_PENALTY=0.081328
PRIMER_LEFT_3_SEQUENCE=TCGAAGATGTCGTTGATGAAAC
PRIMER_RIGHT_3_SEQUENCE=GTCGACGGTAGCTGAGAAGACT
PRIMER_LEFT_3=383,22
PRIMER_RIGHT_3=610,22
PRIMER_LEFT_3_TM=60.118
PRIMER_RIGHT_3_TM=60.081
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=0.2060
PRIMER_LEFT_4_PENALTY=0.124655
PRIMER_RIGHT_4_PENALTY=0.081328
PRIMER_LEFT_4_SEQUENCE=CTTCTCGAAGATGTCGTTGATG
PRIMER_RIGHT_4_SEQUENCE=GTCGACGGTAGCTGAGAAGACT
PRIMER_LEFT_4=379,22
PRIMER_RIGHT_4=610,22
PRIMER_LEFT_4_TM=59.875
PRIMER_RIGHT_4_TM=60.081
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_03003 Ps_contig_mira-and-tgicl-ass_franssen_28158
SEQUENCE=CGGTTAAACACACNCTTTCGTATTCATTATAAAAAAGGGTATTCATTTGATTTTAAAAGAACATTACACACTAATCTATATCTAATTCCAAGACATAAACATCACCAAGAACCACATCAGAAACAACCCTACAAATTAAACAGTTTAAGCACTTGTAAACTTAGTAACAGCTTTTGTTCCTTCAGAGACGGCATGCTTGGCCAATTCTCCAGGAAGAACAAGCCTAACTGCAGTCTGAATTTCCCTTGAAGTGATCGTCGGCTTCTTGTTGTATCTTGCAAGTCTGGAAGATTCGGCAGCAAGCTTCTCGAAGATGTCGTTAATGAAACTGTTCATGATTCCCATGGCCTTGCTTGAGATACCAATGTCTGGGTGAACTTGCTTCAGAACTTTGAAGATGTAGATCTTGTATGTCTCTACGTTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCAGCGGCACCTCCGTCCTTGGGAAGCTTCTTTCCGGCCTTTGGCTTCTTCTCCGCCGGTGCTTTCTCAGCTACCGTAGATTTCTTCTCCTCTGCGGGCTTCTTCTCTGCTATCTTCTTCTCTCCCTTTGGCGCCATTGAAATAATTGAGAATTTGAAATTTCAGAAAAAAGTTAGGGTTTGGAATTGGAAGTGAAATAAGACGCAGGTAATGAGTTTC
TARGET=435,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3183
PRIMER_LEFT_PENALTY=0.108684
PRIMER_RIGHT_PENALTY=0.209637
PRIMER_LEFT_SEQUENCE=TACCAATGTCTGGGTGAACTTG
PRIMER_RIGHT_SEQUENCE=CACTTCCAATTCCAAACCCTAA
PRIMER_LEFT=359,22
PRIMER_RIGHT=647,22
PRIMER_LEFT_TM=59.891
PRIMER_RIGHT_TM=60.210
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.3419
PRIMER_LEFT_1_PENALTY=0.132262
PRIMER_RIGHT_1_PENALTY=0.209637
PRIMER_LEFT_1_SEQUENCE=TGCTTGAGATACCAATGTCTGG
PRIMER_RIGHT_1_SEQUENCE=CACTTCCAATTCCAAACCCTAA
PRIMER_LEFT_1=350,22
PRIMER_RIGHT_1=647,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=60.210
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.3569
PRIMER_LEFT_2_PENALTY=0.147295
PRIMER_RIGHT_2_PENALTY=0.209637
PRIMER_LEFT_2_SEQUENCE=TTGAGATACCAATGTCTGGGTG
PRIMER_RIGHT_2_SEQUENCE=CACTTCCAATTCCAAACCCTAA
PRIMER_LEFT_2=353,22
PRIMER_RIGHT_2=647,22
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=60.210
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.5528
PRIMER_LEFT_3_PENALTY=0.108684
PRIMER_RIGHT_3_PENALTY=0.444125
PRIMER_LEFT_3_SEQUENCE=TACCAATGTCTGGGTGAACTTG
PRIMER_RIGHT_3_SEQUENCE=CTACGGTAGCTGAGAAAGCACC
PRIMER_LEFT_3=359,22
PRIMER_RIGHT_3=532,22
PRIMER_LEFT_3_TM=59.891
PRIMER_RIGHT_3_TM=60.444
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.5764
PRIMER_LEFT_4_PENALTY=0.132262
PRIMER_RIGHT_4_PENALTY=0.444125
PRIMER_LEFT_4_SEQUENCE=TGCTTGAGATACCAATGTCTGG
PRIMER_RIGHT_4_SEQUENCE=CTACGGTAGCTGAGAAAGCACC
PRIMER_LEFT_4=350,22
PRIMER_RIGHT_4=532,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=60.444
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_03004 Ps_contig_mira-and-tgicl-ass_franssen_28159
SEQUENCE=AAGCAGTAGGTAATACAAACGCAGAGTACGGGGAAGAGAATCAGTCGNGATGATCTCGTTTCAGTTTTAATATCCAATTTCCAATTCGAAACCCTAAATTTCTCAATTCTTGTAATGGCGCCAAAGGGAGAGAAGAAGCCTGCTGAGAAGAAACCCGTCGAAGAGAAGAAGTCTACGGTGGCCGAGAAGGCTCCTGCCGAGAAGAAACCCAAGGCCGGAAAGAAGCTTCCCAAGGAAGCCGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAATAAGAAGAGTGTGGAGACATACAAGATCTACATCTTCAAAGTTCTGAAGCAGGTTCACCCTGACATCGGTATCTCAAGCAAGGCCATGGGAATCATGAACAGCTTCATCAACGACATCTTCGAGAAGCTTGCTGCTGAATCTTCCAGACTTGCAAGGTACAACAAGAAGCCGACAATAACATCTAGGGAAATTCAGACGGCAGTCAGGCTTGTTCTGCCTGGGGAATTGGCTAAGCATGCTGTTTCAGAGGGAACCAAAGCTGTGACTAAGTTTACAAGTGCTTGAATTATGTATAATTTGCTTTGTACTTTGTAATGGTTATTCTAAGATTATTTCTGTTAGTGTTAGGTCTGAATTAGTGTGAAACTTTCTTTTAAATCAAATGAATCCTTCTGTGTATGAATGAATCTCTTTTGCTTAAGCAATTGTTACGGAAAAACTT
TARGET=259,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0931
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.080962
PRIMER_LEFT_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_RIGHT_SEQUENCE=TGATGAAGCTGTTCATGATTCC
PRIMER_LEFT=153,22
PRIMER_RIGHT=384,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.0985
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_RIGHT_1_SEQUENCE=TGTACCTTGCAAGTCTGGAAGA
PRIMER_LEFT_1=153,22
PRIMER_RIGHT_1=435,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.1303
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=0.118120
PRIMER_LEFT_2_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_RIGHT_2_SEQUENCE=GAAGATGTCGTTGATGAAGCTG
PRIMER_LEFT_2=153,22
PRIMER_RIGHT_2=395,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=59.882
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=243
PRIMER_PAIR_PENALTY_3=0.1368
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_RIGHT_3_SEQUENCE=CTCGAAGATGTCGTTGATGAAG
PRIMER_LEFT_3=153,22
PRIMER_RIGHT_3=398,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=0.1368
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=CCCGTCGAAGAGAAGAAGTCTA
PRIMER_RIGHT_4_SEQUENCE=CTTCTCGAAGATGTCGTTGATG
PRIMER_LEFT_4=153,22
PRIMER_RIGHT_4=401,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_03005 Ps_contig_mira-and-tgicl-ass_franssen_28160
SEQUENCE=ACATGTATCACGCAGANTAGACTCATTCACACTTGTTCAATCTGCATCTTCCATATTCTTTCAATTTCCAATTTCCATTTCCCATTTCCCAAACAAAAATGGCACCAAAAGGAGAGAAGAAGCCAGCCGAGAAGAAACCTGTAGAGGAGAAGAAATCCACAATAGCCGAGAAGACTCCCGCCGAGAAGAAGCCAAAGGCCGGAAAGAAGCTACCCAAGGACGGTGGCGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGTGTGGAGACCTACAAGATCTACATCTTCAAGGTTCTGAAACAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGTATAATGACAGTTTCATCAATGATATCTTTGAGAAGCTTGCTG
TARGET=243,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2427
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.147295
PRIMER_LEFT_SEQUENCE=AAATCCACAATAGCCGAGAAGA
PRIMER_RIGHT_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT=152,22
PRIMER_RIGHT=335,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.853
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.2427
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.147295
PRIMER_LEFT_1_SEQUENCE=GAAATCCACAATAGCCGAGAAG
PRIMER_RIGHT_1_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_1=151,22
PRIMER_RIGHT_1=335,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=59.853
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.2427
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.147295
PRIMER_LEFT_2_SEQUENCE=AGAAATCCACAATAGCCGAGAA
PRIMER_RIGHT_2_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_2=150,22
PRIMER_RIGHT_2=335,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=59.853
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=0.2427
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.147295
PRIMER_LEFT_3_SEQUENCE=GAAGAAATCCACAATAGCCGAG
PRIMER_RIGHT_3_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_3=148,22
PRIMER_RIGHT_3=335,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=59.853
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=0.2427
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=0.147295
PRIMER_LEFT_4_SEQUENCE=AAGAAATCCACAATAGCCGAGA
PRIMER_RIGHT_4_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_4=149,22
PRIMER_RIGHT_4=335,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=59.853
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_03006 Ps_contig_mira-and-tgicl-ass_franssen_28161
SEQUENCE=TTCACTCCCAATTTCTGCATCCTCTTCGATTTCTAAGTTGTATCCCTAAACTTTTCCAAAGTAGAATTTCTGAATTCCTTCAATGGCGCCAAAGGGAGAGAAGAAACCAGCTGAGAAGAAACCCGTGGAGGAGAAGAAGTCCACGGTAGCAGAGAAAGCCCCGGCCGAGAAGAAGCCAAAGGCCGGAAAGAAGCTGCCTAAGGATGGCGGTGCCGCTGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGTGTGGAGACATACAAGATCTACATCTTCAAGGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGTATCATGAACAGTTTCATCAACGACATCTTCGAGAAGCTTGCTGCAGAGTCTTCAAGACCTGCAAGATGCAACAAGAAGCCAACAATTACATCAAGGGAAATTCAGACCG
TARGET=227,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0871
PRIMER_LEFT_PENALTY=0.056850
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=AAGTCCACGGTAGCAGAGAAAG
PRIMER_RIGHT_SEQUENCE=TGAACTTGCTTCAGAACCTTGA
PRIMER_LEFT=136,22
PRIMER_RIGHT=299,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.1620
PRIMER_LEFT_1_PENALTY=0.131699
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=GGAGAGAAGAAACCAGCTGAGA
PRIMER_RIGHT_1_SEQUENCE=TGAACTTGCTTCAGAACCTTGA
PRIMER_LEFT_1=94,22
PRIMER_RIGHT_1=299,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=0.1747
PRIMER_LEFT_2_PENALTY=0.056850
PRIMER_RIGHT_2_PENALTY=0.117817
PRIMER_LEFT_2_SEQUENCE=AAGTCCACGGTAGCAGAGAAAG
PRIMER_RIGHT_2_SEQUENCE=TCGAAGATGTCGTTGATGAAAC
PRIMER_LEFT_2=136,22
PRIMER_RIGHT_2=365,22
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=60.118
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.1815
PRIMER_LEFT_3_PENALTY=0.056850
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=AAGTCCACGGTAGCAGAGAAAG
PRIMER_RIGHT_3_SEQUENCE=CTTCTCGAAGATGTCGTTGATG
PRIMER_LEFT_3=136,22
PRIMER_RIGHT_3=369,22
PRIMER_LEFT_3_TM=59.943
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.2041
PRIMER_LEFT_4_PENALTY=0.056850
PRIMER_RIGHT_4_PENALTY=0.147295
PRIMER_LEFT_4_SEQUENCE=AAGTCCACGGTAGCAGAGAAAG
PRIMER_RIGHT_4_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_4=136,22
PRIMER_RIGHT_4=319,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=59.853
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_03007 Ps_contig_mira-and-tgicl-ass_franssen_28162
SEQUENCE=CANAGTCGGGGACTCATTCACACTTGTTCAATCTGCATCTTCCATATTCTTTCAATTTTCAATTTCCATTTCCCAAACAAAAATGGCACCAAAAGGAGAGAAGAAGCCAGCCGAGAAGAAACCTGTAGAGGAGAAGAAATCCACAATAGCCGAGAAGACTCCCGCCGAGAAGAAGCCAAAGGCCGGAAAGAAGCTACCCAAGGACGGTGGCGCCGCTGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGTGTGGAGACCTACAAGATCTACATCTTTAAGGTTCTGAAACAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGTATAATGAACAGTTTCATCAACGATATCTTTGAGAAGCTTGCTGCTGAATCTTCGAGGCTTGCGAGGTATAACAAGAAGCCAACGATAACTTCGAGGGAGATTCAGACTGCGGTTAGGCTTGTTCTTCCTGGAGAATTGGCCAAGCATGCTGTATCTGAGGGTACCAAAGCTGTAACCAAGTTCACTAGCGTTTAGAGATTTTACAGTGTAGTTGTTGTTTAGAGGATTAATGTTTAACTTGTAAGGTTGTATAATTGAATCTGGTGGATCTTTTGTGTTTTGAAAGAATAGTGGAATTGACTATATTTGGGGTTCGCTTGTTTTATGCANTTGGTGTTCATGTGCT
TARGET=227,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1909
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=AAATCCACAATAGCCGAGAAGA
PRIMER_RIGHT_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_LEFT=136,22
PRIMER_RIGHT=344,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.1909
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=AGAAATCCACAATAGCCGAGAA
PRIMER_RIGHT_1_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_LEFT_1=134,22
PRIMER_RIGHT_1=344,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.1909
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.095445
PRIMER_LEFT_2_SEQUENCE=AAGAAATCCACAATAGCCGAGA
PRIMER_RIGHT_2_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_LEFT_2=133,22
PRIMER_RIGHT_2=344,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.1909
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.095445
PRIMER_LEFT_3_SEQUENCE=GAAATCCACAATAGCCGAGAAG
PRIMER_RIGHT_3_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_LEFT_3=135,22
PRIMER_RIGHT_3=344,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=60.095
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=0.1909
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=0.095445
PRIMER_LEFT_4_SEQUENCE=GAAGAAATCCACAATAGCCGAG
PRIMER_RIGHT_4_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_LEFT_4=132,22
PRIMER_RIGHT_4=344,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=60.095
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_03008 Ps_contig_mira-and-tgicl-ass_franssen_28163
SEQUENCE=TCGGGGACTCATTCGCACTTGTTCGATCTGCATCTTTCATCTTCTTTCAAATTCCAATTTCCAAATTTCCAAACAAAAATGGCACCAAAAGGAGAGAAGAAACCAGCTGAGAAGAAACCTGTAGAGGAGAAGAAATCCACAATAGCCGAGAAGACTCCCGCCGAGAAGAAGCCAAAGGCCGGAAAGAAGCTACCTAAGGACGGTGGCGCCGCTGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAATGTGGAAACCTACAAGATCTACATCTTCAAGGTTCTGAAACAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGTATAATGAACAGTTTCATCAACGATATCTTTGAGAAGCTTGCTGCTGAATCTTCGAGACTTGCGAGGTATAACAAGAAGCCAACGATAACTTCGAGGGAGATTCAGACTGCGGTTAGGCTTGTTCTTCCTGGAGAATTGGCCAAGCATGCTGTATCTGAGGGTACCAAAGCTGTAACCAAGTTCACTAGCGCTTAGAGATTTTACAGTGTAGTTGTTGTTTAGAGGATTCATGTTTAACTTGTAAGGTGATGTAATGTGATCCGGTGGATCTTTTGTGTATTCAAAGAATAGTGGAATTGACTATATTTGGTGTTCRCTTGTTTTGTGAAACG
TARGET=223,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03009 Ps_contig_mira-and-tgicl-ass_franssen_28164
SEQUENCE=AAAGGAGAGAAGAAGCCAGCCGAGAAGAAACCCGTAGAGGAGAAGAAATCCACGGTAGCCGAGAAGGTTCCGGCTGAGAAGAAGCCAAAGGCCGGCAAGAAGCTGCCTAAAGACGGTGGTGCCACCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTGGAGACATACGAGATCTACATCTTCAAAGTTCTGAAGCAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGAATCATGAACAGTTTCATCAATGATATTTTCGAGAAGCTTGCTGCGGAGTCGTCTAGACTTGCGAGGTACAACAAGAAGCCGACGATAACTTCGAGGGAAATTCAGACCGCGGTCAGGCTCGTTCTGCCCGGTGAATTGGCTAAGCATGCTGTTTTCTGGAGGGTAACCAAAGCTGTAACTAAGTTCACTAGTTCTTAGTATTTAGTAAACTTTGGGTTTTTTAGGGGTTT
TARGET=136,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1138
PRIMER_LEFT_PENALTY=0.032476
PRIMER_RIGHT_PENALTY=0.081328
PRIMER_LEFT_SEQUENCE=ACCCGTAGAGGAGAAGAAATCC
PRIMER_RIGHT_SEQUENCE=ACCTCGCAAGTCTAGACGACTC
PRIMER_LEFT=29,22
PRIMER_RIGHT=309,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.1798
PRIMER_LEFT_1_PENALTY=0.032476
PRIMER_RIGHT_1_PENALTY=0.147295
PRIMER_LEFT_1_SEQUENCE=ACCCGTAGAGGAGAAGAAATCC
PRIMER_RIGHT_1_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_1=29,22
PRIMER_RIGHT_1=228,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=59.853
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.3358
PRIMER_LEFT_2_PENALTY=0.254467
PRIMER_RIGHT_2_PENALTY=0.081328
PRIMER_LEFT_2_SEQUENCE=CGTAGAGGAGAAGAAATCCACG
PRIMER_RIGHT_2_SEQUENCE=ACCTCGCAAGTCTAGACGACTC
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=309,22
PRIMER_LEFT_2_TM=60.254
PRIMER_RIGHT_2_TM=60.081
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.4018
PRIMER_LEFT_3_PENALTY=0.254467
PRIMER_RIGHT_3_PENALTY=0.147295
PRIMER_LEFT_3_SEQUENCE=CGTAGAGGAGAAGAAATCCACG
PRIMER_RIGHT_3_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=228,22
PRIMER_LEFT_3_TM=60.254
PRIMER_RIGHT_3_TM=59.853
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=0.4076
PRIMER_LEFT_4_PENALTY=0.032476
PRIMER_RIGHT_4_PENALTY=0.375090
PRIMER_LEFT_4_SEQUENCE=ACCCGTAGAGGAGAAGAAATCC
PRIMER_RIGHT_4_SEQUENCE=TGAGATACCAATGTCAGGGTGA
PRIMER_LEFT_4=29,22
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=59.968
PRIMER_RIGHT_4_TM=60.375
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_03010 Ps_contig_mira-and-tgicl-ass_franssen_28165
SEQUENCE=ATTCACAGTTTTTCGATCTGCTTCATTACATCTTCTTTCAATTTCCAGTTTTCAAATTCCCAAACAGAAATGGCACCAAAAGGAGAGAAGAAGCCCGCCGAGAAGAAACCCGTGGAGGAGAAGAAGTCCACCGTAGCAGAGAAGACTCCTGCCGAGAAGAAGCCAAAGGCCGGGAAGAAGCTGCCTAAGGACGGTGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTAGAGACATACAAGATCTACATCTTCAAAGTTCTGAAACAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGAATCATGAACAGTTTCATCAACGACATTTTCGAGAAGCTTGCTGCTGAGTCTTCAAGACTTGCAAGGTACAACAAGAAGCCAACAATCACTTCAAGGGAAATTCAGACTGCTGTCAGGCTAGTTCTGCCCGGAGAATTGGCCAAACATGCCGTTTCTGAGGGTACCAAGGCTGTAACCAAGTTCACAAGCTCTTAAAATCAAGATTTAGGTTTTGTTTATGTCCCAATGTAGGCACTGTTAGAGTTATTTGTATTCAGCTTTTACCTTTCTAATGTTGTAATGTACTAATGGGATCATGCTGATCCCGGTTGTGTATTAAAATCATGAATTTAATGATATTGGGCTTCAAAATTGCCTCTATTGTTCAATTACTGTTTTTAATATAAGTTTACGGTTCTCTTCAATTYATGCCC
TARGET=214,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03011 Ps_contig_mira-and-tgicl-ass_franssen_28166
SEQUENCE=TCGAAGTTATCGTCGGCTTCTTGTTATACCTCGCAAGCCTCGAAGATTCAGCAGCAAGCTTCTCAAAGATGTCGTTGATGAAACTGTTCATTATACCCATGGCCTTGCTTGAGGTACCAATGTCAGGGTGAACTTGTTTCAGAACCTTGAAGATGTAGATCTTGTAGGTCTCCACACTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCGGCGGCGCCACCGTCCTTGGGTAGCTTCTTTCCGGCCTTGGGTTTCTTCTCGGCGGGAGACTTTTCGGCTATTGTGGATTTCTT
TARGET=190,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1256
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.030114
PRIMER_LEFT_SEQUENCE=CTGTTCATTATACCCATGGCCT
PRIMER_RIGHT_SEQUENCE=GAAATCCACAATAGCCGAAAAG
PRIMER_LEFT=83,22
PRIMER_RIGHT=292,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.970
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.1793
PRIMER_LEFT_1_PENALTY=0.149184
PRIMER_RIGHT_1_PENALTY=0.030114
PRIMER_LEFT_1_SEQUENCE=GTCGGCTTCTTGTTATACCTCG
PRIMER_RIGHT_1_SEQUENCE=GAAATCCACAATAGCCGAAAAG
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=292,22
PRIMER_LEFT_1_TM=60.149
PRIMER_RIGHT_1_TM=59.970
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=0.1793
PRIMER_LEFT_2_PENALTY=0.149184
PRIMER_RIGHT_2_PENALTY=0.030114
PRIMER_LEFT_2_SEQUENCE=CGTCGGCTTCTTGTTATACCTC
PRIMER_RIGHT_2_SEQUENCE=GAAATCCACAATAGCCGAAAAG
PRIMER_LEFT_2=10,22
PRIMER_RIGHT_2=292,22
PRIMER_LEFT_2_TM=60.149
PRIMER_RIGHT_2_TM=59.970
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.1793
PRIMER_LEFT_3_PENALTY=0.149184
PRIMER_RIGHT_3_PENALTY=0.030114
PRIMER_LEFT_3_SEQUENCE=TCGTCGGCTTCTTGTTATACCT
PRIMER_RIGHT_3_SEQUENCE=GAAATCCACAATAGCCGAAAAG
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=292,22
PRIMER_LEFT_3_TM=60.149
PRIMER_RIGHT_3_TM=59.970
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.1833
PRIMER_LEFT_4_PENALTY=0.153161
PRIMER_RIGHT_4_PENALTY=0.030114
PRIMER_LEFT_4_SEQUENCE=GGTACCAATGTCAGGGTGAACT
PRIMER_RIGHT_4_SEQUENCE=GAAATCCACAATAGCCGAAAAG
PRIMER_LEFT_4=112,22
PRIMER_RIGHT_4=292,22
PRIMER_LEFT_4_TM=60.153
PRIMER_RIGHT_4_TM=59.970
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_03012 Ps_contig_mira-and-tgicl-ass_franssen_28167
SEQUENCE=CGTCGGGACAGTTTTTCGATCTGCTTCATTACATCTTCTTTCAATTTCCAGTTTTCAAATTCCCAAACAGAAATGGCACCAAAAGGAGAGAAGAAGCCCGCCGAGAAGAAACCCGTGGAGGAGAAGAAGTCCACCGTAGCAGAGAAGACTCCTGCCGAGAAGAAGCCAAAGGCCGGGAAGAAGCTGCCTAAGGACGGTGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTAGAGACATACGAGATCTACATCTTCAAAGTTCTGAAACAAGTTCACCCTGACATTGGTATCTCAAGCAAGGCCATGGGAATCATGAACAGTTTCATCAACGACATATTCGAGAAGCTTGCTGCAGAATCTTCGAGGCTTGCAAGATACAACAAGAAGCCAACGATCACTTCCAGGGAAATTCAGACCGCAGTCAGGCTTGTTCTGCCCGGTGAATTGGCGAAGCATGCCGTTTCTGAGGGCACCAAAGCCGTAACCAAGTTCACAAGCTCTTAGACTTGAATTAA
TARGET=217,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1341
PRIMER_LEFT_PENALTY=0.074231
PRIMER_RIGHT_PENALTY=0.059875
PRIMER_LEFT_SEQUENCE=ACCGTAGCAGAGAAGACTCCTG
PRIMER_RIGHT_SEQUENCE=TCTGAATTTCCCTGGAAGTGAT
PRIMER_LEFT=132,22
PRIMER_RIGHT=429,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.940
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=0.2026
PRIMER_LEFT_1_PENALTY=0.055352
PRIMER_RIGHT_1_PENALTY=0.147295
PRIMER_LEFT_1_SEQUENCE=AGTCCACCGTAGCAGAGAAGAC
PRIMER_RIGHT_1_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_1=127,22
PRIMER_RIGHT_1=309,22
PRIMER_LEFT_1_TM=59.945
PRIMER_RIGHT_1_TM=59.853
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.2026
PRIMER_LEFT_2_PENALTY=0.055352
PRIMER_RIGHT_2_PENALTY=0.147295
PRIMER_LEFT_2_SEQUENCE=GTCCACCGTAGCAGAGAAGACT
PRIMER_RIGHT_2_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_2=128,22
PRIMER_RIGHT_2=309,22
PRIMER_LEFT_2_TM=59.945
PRIMER_RIGHT_2_TM=59.853
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=182
PRIMER_PAIR_PENALTY_3=0.2215
PRIMER_LEFT_3_PENALTY=0.074231
PRIMER_RIGHT_3_PENALTY=0.147295
PRIMER_LEFT_3_SEQUENCE=ACCGTAGCAGAGAAGACTCCTG
PRIMER_RIGHT_3_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_LEFT_3=132,22
PRIMER_RIGHT_3=309,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.853
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=178
PRIMER_PAIR_PENALTY_4=0.2406
PRIMER_LEFT_4_PENALTY=0.055352
PRIMER_RIGHT_4_PENALTY=0.185267
PRIMER_LEFT_4_SEQUENCE=AGTCCACCGTAGCAGAGAAGAC
PRIMER_RIGHT_4_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_LEFT_4=127,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=59.945
PRIMER_RIGHT_4_TM=60.185
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_03013 Ps_contig_mira-and-tgicl-ass_franssen_28168
SEQUENCE=ACATTACAACATTAGAAGGTAAAAGACTGAATACAAATAACTCTAACAGTGCCTACATTGGGACATAAACAAAACCTAAAATCTTGATTTTAAGAGCTTGTGAACTTGGTTACAGCCTTGGTACCTTCAGAAACGGCATGTTTGGCCAATTCTCCGGGCAGAACTAGCCTGACAGCAGTCTGAATTTCCCTTGAAGTGATTGTTGGCTTCTTGTTGTACCTTGCAAGTCTTGAAGACTCAGCAGCAAGCTTCTCGAAAATGTCGTTGATGAAACTGTTCATGATACCCATGGCCTTGCTTGAGATACCAATGTCAGGGTGAACTTGTTTCAGAACTTTGAAGATGTAGATCTTGTATGTCTCCACGCTCTTCTTGTTTCTCTTCTTCTTCTTGTCTCCGGCGGCGGCACCACCGTCCTTAGGCAGCTTCTTTCCGGCCTTTGGCTTCTTCTCGGCAGGAGCCTTCTCTGCTATGGTGGACTTCTTCTCCTCCACGGGTTTCTTCTCCGCTGGCTTCTTCTCTCCTTTTGGTGCCATTTCTGTTTGGGAATTTGAAAACTGAAAATTGAAATAAGATGTAAAGATGCAGATCGAAAAACTGTGAATCCCCCGAACNCTGCGTTGAT
TARGET=380,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3743
PRIMER_LEFT_PENALTY=0.147295
PRIMER_RIGHT_PENALTY=0.226971
PRIMER_LEFT_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_RIGHT_SEQUENCE=AAGTCCACCATAGCAGAGAAGG
PRIMER_LEFT=298,22
PRIMER_RIGHT=481,22
PRIMER_LEFT_TM=59.853
PRIMER_RIGHT_TM=59.773
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.4122
PRIMER_LEFT_1_PENALTY=0.185267
PRIMER_RIGHT_1_PENALTY=0.226971
PRIMER_LEFT_1_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_1_SEQUENCE=AAGTCCACCATAGCAGAGAAGG
PRIMER_LEFT_1=310,22
PRIMER_RIGHT_1=481,22
PRIMER_LEFT_1_TM=60.185
PRIMER_RIGHT_1_TM=59.773
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.4421
PRIMER_LEFT_2_PENALTY=0.185267
PRIMER_RIGHT_2_PENALTY=0.256829
PRIMER_LEFT_2_SEQUENCE=TGTCAGGGTGAACTTGTTTCAG
PRIMER_RIGHT_2_SEQUENCE=TCACAGTTTTTCGATCTGCATC
PRIMER_LEFT_2=310,22
PRIMER_RIGHT_2=602,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=60.257
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.4805
PRIMER_LEFT_3_PENALTY=0.253482
PRIMER_RIGHT_3_PENALTY=0.226971
PRIMER_LEFT_3_SEQUENCE=CTTCTCGAAAATGTCGTTGATG
PRIMER_RIGHT_3_SEQUENCE=AAGTCCACCATAGCAGAGAAGG
PRIMER_LEFT_3=248,22
PRIMER_RIGHT_3=481,22
PRIMER_LEFT_3_TM=59.747
PRIMER_RIGHT_3_TM=59.773
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.5667
PRIMER_LEFT_4_PENALTY=0.309858
PRIMER_RIGHT_4_PENALTY=0.256829
PRIMER_LEFT_4_SEQUENCE=ATGTCTCCACGCTCTTCTTGTT
PRIMER_RIGHT_4_SEQUENCE=TCACAGTTTTTCGATCTGCATC
PRIMER_LEFT_4=355,22
PRIMER_RIGHT_4=602,22
PRIMER_LEFT_4_TM=60.310
PRIMER_RIGHT_4_TM=60.257
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_03014 Ps_contig_mira-and-tgicl-ass_franssen_28169
SEQUENCE=GTCGGGGAAGCTATTTTTCTGCGTTTTATTTCACTTCCAATTTCAAACCCTAACTTTTTCTGAAATTTCAAATTCTCAATTCCTTCAATGGCGCCAAAGGGAGAGAAGAAGATAGCAGAGAAGAAACCAGTAGAAGAGAAGAAGTCTACAGTAGCTGAGAAAGCTCCGGCTGAAAAGAAGCCAAAGGCCGGAAAGAAGCTTCCCAAGGACGGTGGTGCTGCCGCCGGAGACAAGAAGAAGAAGAGAAACAAGAAGAACGTAGAGACATACAAGATCTACATCTTCAAAGTTCTGAAGCAAGTTCACCCAGACATTGGAATCTCAAGCAAGGCCATGGGGATCATGAACAGTTTCATTAACGACATCTTCGAGAAGCTTGCTGCCGAATCTTCCAGACTTGCAAGATACAACAAGAAGCCGACGATCACTTCAAGGGAAATCCAGACTGCAGTTAGGCTTGTTCTTCCTGGAGAATTGGCCAAGCATGCTGTCTCTGAAGGAACAAAGGCTGTTACCAAGTTTACGAGTTCTTAAATTGTATAATTTGTAGGGTTGTTTCTGAAGTGTTTCTTCCTAAAGTTTATGTCTCTTGGAAATTAGATCTAGATTAGTGTGTAATGTTCTTTTAAAAATCAAATTAATACCTTTTCTATGAGTCC
TARGET=232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0793
PRIMER_LEFT_PENALTY=1.019107
PRIMER_RIGHT_PENALTY=0.060196
PRIMER_LEFT_SEQUENCE=GCCAAAGGGAGAGAAGAAGATA
PRIMER_RIGHT_SEQUENCE=AATGAAACTGTTCATGATCCCC
PRIMER_LEFT=92,22
PRIMER_RIGHT=356,22
PRIMER_LEFT_TM=58.981
PRIMER_RIGHT_TM=60.060
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=1.1022
PRIMER_LEFT_1_PENALTY=1.048901
PRIMER_RIGHT_1_PENALTY=0.053348
PRIMER_LEFT_1_SEQUENCE=TACAGTAGCTGAGAAAGCTCCG
PRIMER_RIGHT_1_SEQUENCE=GATTTCCCTTGAAGTGATCGTC
PRIMER_LEFT_1=146,22
PRIMER_RIGHT_1=440,22
PRIMER_LEFT_1_TM=58.951
PRIMER_RIGHT_1_TM=59.947
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=1.1091
PRIMER_LEFT_2_PENALTY=1.048901
PRIMER_RIGHT_2_PENALTY=0.060196
PRIMER_LEFT_2_SEQUENCE=TACAGTAGCTGAGAAAGCTCCG
PRIMER_RIGHT_2_SEQUENCE=AATGAAACTGTTCATGATCCCC
PRIMER_LEFT_2=146,22
PRIMER_RIGHT_2=356,22
PRIMER_LEFT_2_TM=58.951
PRIMER_RIGHT_2_TM=60.060
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=1.1850
PRIMER_LEFT_3_PENALTY=1.131668
PRIMER_RIGHT_3_PENALTY=0.053348
PRIMER_LEFT_3_SEQUENCE=CTACAGTAGCTGAGAAAGCTCCG
PRIMER_RIGHT_3_SEQUENCE=GATTTCCCTTGAAGTGATCGTC
PRIMER_LEFT_3=145,23
PRIMER_RIGHT_3=440,22
PRIMER_LEFT_3_TM=59.868
PRIMER_RIGHT_3_TM=59.947
PRIMER_LEFT_3_GC_PERCENT=52.174
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=1.1863
PRIMER_LEFT_4_PENALTY=1.126080
PRIMER_RIGHT_4_PENALTY=0.060196
PRIMER_LEFT_4_SEQUENCE=GCCAAAGGGAGAGAAGAAGATAG
PRIMER_RIGHT_4_SEQUENCE=AATGAAACTGTTCATGATCCCC
PRIMER_LEFT_4=92,23
PRIMER_RIGHT_4=356,22
PRIMER_LEFT_4_TM=59.874
PRIMER_RIGHT_4_TM=60.060
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_03015 Ps_contig_mira-and-tgicl-ass_franssen_28170
SEQUENCE=TTCCTTGTTGTACCTTCGTCAAGCCTTGAAGATTCTGCAGCAAGTTTCTCAAAGATGTCGTTGATGAAACTGTTCATGATCCCCATGGCCTTGCTTGAGATACCAATGTCAGGGTGAACTTGCTTCAGAACTTTGAAAATGTAGATCTTGTATGTCTCTACGCTGTTCTTGTTTCTCTTCTTCTTCTTGTCACCGGCGGCGGCACCGCCGTCCTTTGGTAGCTTCTTTCCGGCCTTTGGCTTCTTCTCGGCCGGAGCTTTTTCTGGTACTGTAGACTTCTTCTCCTCTGCAGGTTTCTTCTCCGCGGGCTTCTTCTCTCCCTTTGGCGCCATTGAAGAAATTGGTAATTTGAAACTTCAGAAAAATTAGGGTTTCGAATTGGAAATGAGTTTCAAA
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2790
PRIMER_LEFT_PENALTY=0.147295
PRIMER_RIGHT_PENALTY=0.131699
PRIMER_LEFT_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_RIGHT_SEQUENCE=GAGAAGAAACCTGCAGAGGAGA
PRIMER_LEFT=94,22
PRIMER_RIGHT=301,22
PRIMER_LEFT_TM=59.853
PRIMER_RIGHT_TM=60.132
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.3351
PRIMER_LEFT_1_PENALTY=0.203367
PRIMER_RIGHT_1_PENALTY=0.131699
PRIMER_LEFT_1_SEQUENCE=TGATGAAACTGTTCATGATCCC
PRIMER_RIGHT_1_SEQUENCE=GAGAAGAAACCTGCAGAGGAGA
PRIMER_LEFT_1=61,22
PRIMER_RIGHT_1=301,22
PRIMER_LEFT_1_TM=59.797
PRIMER_RIGHT_1_TM=60.132
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.5068
PRIMER_LEFT_2_PENALTY=0.375090
PRIMER_RIGHT_2_PENALTY=0.131699
PRIMER_LEFT_2_SEQUENCE=TGAGATACCAATGTCAGGGTGA
PRIMER_RIGHT_2_SEQUENCE=GAGAAGAAACCTGCAGAGGAGA
PRIMER_LEFT_2=95,22
PRIMER_RIGHT_2=301,22
PRIMER_LEFT_2_TM=60.375
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.5093
PRIMER_LEFT_3_PENALTY=0.147295
PRIMER_RIGHT_3_PENALTY=0.361969
PRIMER_LEFT_3_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_RIGHT_3_SEQUENCE=GAAACCTGCAGAGGAGAAGAAG
PRIMER_LEFT_3=94,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=59.853
PRIMER_RIGHT_3_TM=59.638
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.5093
PRIMER_LEFT_4_PENALTY=0.147295
PRIMER_RIGHT_4_PENALTY=0.361969
PRIMER_LEFT_4_SEQUENCE=TTGAGATACCAATGTCAGGGTG
PRIMER_RIGHT_4_SEQUENCE=AGAAACCTGCAGAGGAGAAGAA
PRIMER_LEFT_4=94,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=59.853
PRIMER_RIGHT_4_TM=59.638
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=204
=
PRIMER_SEQUENCE_ID=SSR_03016 Ps_contig_mira-and-tgicl-ass_franssen_28202
SEQUENCE=GGAGAAATAGACTTATTCAGAAAATTTCCTACAAAAGAAAAATAAAAAGTAGTTAGACNGAAATTTCGCTACTCGAGTGGCCAGAGTGAGATTGAAACATAAACGGCGACGTTTGATATCTGATAAACTAGCACTACACACGGCCACGTCACCCTCCTCTCTCGACCAGGATACAGAAAAACAATGGCGAAGCCAGAGATCACTAGCAGAGGCTCAAGGTGGTCCCTCGCCGGATTCACAGCTCTAGTCACCGGTGGAACGCGTGGAATCGGGCATGCTGTAGTGGAGGAACTAGCCGAGTTTGGTGCCACAGTGTATACTTGTTCTAGGAATGAAGAAGAGCTTAATAAATGCTTAAATGAATGGAAAAGTAAGGGTTTTTCGGTTAATGGATCGGTATGTGACGCGTCTTCTTCTTCTCAAAGAGACGAGCTTATTCGACAAGTGGCTTCTTCCTTCAACGGCAAGCTCAACATACTTGTAAACAATGTTGGAACGAATGTGAGGAAGCCGACAATTGAGTATACAACCGAAGATTATTCGAAAGTGATGGCTACTAACTTGGATTCTGTGTACCATCTATGCCAAGTGGCGTATCCTCTTCTCAAAGAATCTGGAAATGGAAGCATTGTGTTTATTTCCTCGGTTGCATCTCTCACGAGTGTAGGTTCTGGAACCATCTATGCAGCGAGTAAAGCTGCAATCAATCAGCTGACAAAAAGTCTTGCTTGTGAATGGGCAAAAGATAATATAAGGAGCAACTGTGTTGCGCCGTGGTATACAAAAACACCACTTGTGGAACATTTAATTGCTAACGAAGAGTTTGTGAATGAAGTGCTATCTCGAACACCGATAAAGCGGATAGCAGAAACACATGAAGTGTCTTCCTTGGTGACTTTCCTTTGTCTGCCTGCTGCTTCCTACATCACTGGACAGATTGTTTCTGTTGATGGAGGATTTACTGTGAATGGATTTCAACCCAGCATGAGGATTACCTAAAGCAAGAATGTTCGTTTTTTATTTTCAGCATTGTTTCCACAACATAAATAATGAACAAAGGAATGAATTGGGTAAAGTTAATGAAAGGGGATGAAGATTAACAACTCAGTTTTAGAATCATCTTCAGTGGTAAATGTTTCAAATGTATTTTTCACTAGAATCATCTTGCCTGCATGTTTCTCGCAGAAACTGAATATACTGTTGAACATATTCTAGTTCTTCATATTTCTCTTGATGTTGTATTGAAGCATTAATGAAACTCCGGAGTTATTTACGAAGAATATTNANNTATTTTTAATTAAAACC
TARGET=409,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2554
PRIMER_LEFT_PENALTY=0.174518
PRIMER_RIGHT_PENALTY=0.080887
PRIMER_LEFT_SEQUENCE=AGCCAGAGATCACTAGCAGAGG
PRIMER_RIGHT_SEQUENCE=AGAAGCCACTTGTCGAATAAGC
PRIMER_LEFT=190,22
PRIMER_RIGHT=452,22
PRIMER_LEFT_TM=60.175
PRIMER_RIGHT_TM=59.919
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.2554
PRIMER_LEFT_1_PENALTY=0.174518
PRIMER_RIGHT_1_PENALTY=0.080887
PRIMER_LEFT_1_SEQUENCE=AGCCAGAGATCACTAGCAGAGG
PRIMER_RIGHT_1_SEQUENCE=AAGCCACTTGTCGAATAAGCTC
PRIMER_LEFT_1=190,22
PRIMER_RIGHT_1=450,22
PRIMER_LEFT_1_TM=60.175
PRIMER_RIGHT_1_TM=59.919
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.2554
PRIMER_LEFT_2_PENALTY=0.174518
PRIMER_RIGHT_2_PENALTY=0.080887
PRIMER_LEFT_2_SEQUENCE=CCAGAGATCACTAGCAGAGGCT
PRIMER_RIGHT_2_SEQUENCE=AGAAGCCACTTGTCGAATAAGC
PRIMER_LEFT_2=192,22
PRIMER_RIGHT_2=452,22
PRIMER_LEFT_2_TM=60.175
PRIMER_RIGHT_2_TM=59.919
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.3084
PRIMER_LEFT_3_PENALTY=0.233369
PRIMER_RIGHT_3_PENALTY=0.075027
PRIMER_LEFT_3_SEQUENCE=GGAAAAGTAAGGGTTTTTCGGT
PRIMER_RIGHT_3_SEQUENCE=GCTTCCATTTCCAGATTCTTTG
PRIMER_LEFT_3=364,22
PRIMER_RIGHT_3=626,22
PRIMER_LEFT_3_TM=59.767
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=0.3288
PRIMER_LEFT_4_PENALTY=0.233369
PRIMER_RIGHT_4_PENALTY=0.095445
PRIMER_LEFT_4_SEQUENCE=GGAAAAGTAAGGGTTTTTCGGT
PRIMER_RIGHT_4_SEQUENCE=AGAGATGCAACCGAGGAAATAA
PRIMER_LEFT_4=364,22
PRIMER_RIGHT_4=655,22
PRIMER_LEFT_4_TM=59.767
PRIMER_RIGHT_4_TM=60.095
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_03017 Ps_contig_mira-and-tgicl-ass_franssen_28205
SEQUENCE=TGATATCTGGAATGCATACAGTTGAAGACATATTTTGTTGCTGCTGTGGACAAATTGTTGGATGGAAATATATTATAGCACATGAGAAGGGGGAAGCAATATTAGTTTCATCTACAAAACAAGCAAAGAAAGAGAGAATGTTGAGATCCCAGGACTACCACAACCACATTGCAGGAGTCAGAAGGGAGCCAAGTGTTCTCTCCGACTTCGACCGCTACCCAAACGCTGAAATGGCCTTCAACAAGAGGGTTGTCTACGATGAGGTCGAGGGATCCCACCGCCACCACCACCACCACAACCCTGAGATCGTTGAGAGGGTTGAAGTGGTGGAGTATGAACGAGTACCTGAGGTTAGGCCATATGGTGGTGAAGTGATCTATGAAGAGTACATGGATGTTGAAACCAATCAGTATAACCCACGAAGGAACAGGCCAAATGGTCTTGGATTGCAAAAATGGAAAACATACAGAGCATAAGCTTGTGCTTTGTGTTGTGGCTAATTTGCTAGGCAGGCTAAACCCTAACATATGATCACTGTGTGTGGTGGTTGTATTAAATTAAATAAATTCCTGCTTAGTTAGCTTGTTGTTATTTATGTTATAAATCATGTATGAATGATTAATAAATCATATCTATAGCATGTATTTGAATTAATTAATATGTTATGGGCTATTGCACTGTTTATTTAAACTGTTGTTGTTTAGAGTTCTTGTTCAAACTCGTTTCAGCCAATCTGGTATATACTCTCAACTGGTGCCACTTTCGTTTTTCTTTTTGTCTGTAAACATCT
TARGET=281,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0887
PRIMER_LEFT_PENALTY=0.058463
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=GAGAATGTTGAGATCCCAGGAC
PRIMER_RIGHT_SEQUENCE=CGTTCATACTCCACCACTTCAA
PRIMER_LEFT=133,22
PRIMER_RIGHT=339,22
PRIMER_LEFT_TM=59.942
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.0896
PRIMER_LEFT_1_PENALTY=0.058463
PRIMER_RIGHT_1_PENALTY=0.031122
PRIMER_LEFT_1_SEQUENCE=GAGAATGTTGAGATCCCAGGAC
PRIMER_RIGHT_1_SEQUENCE=TACTCCACCACTTCAACCCTCT
PRIMER_LEFT_1=133,22
PRIMER_RIGHT_1=333,22
PRIMER_LEFT_1_TM=59.942
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.1041
PRIMER_LEFT_2_PENALTY=0.073866
PRIMER_RIGHT_2_PENALTY=0.030276
PRIMER_LEFT_2_SEQUENCE=GAGAGAATGTTGAGATCCCAGG
PRIMER_RIGHT_2_SEQUENCE=CGTTCATACTCCACCACTTCAA
PRIMER_LEFT_2=131,22
PRIMER_RIGHT_2=339,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=60.030
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.1050
PRIMER_LEFT_3_PENALTY=0.073866
PRIMER_RIGHT_3_PENALTY=0.031122
PRIMER_LEFT_3_SEQUENCE=GAGAGAATGTTGAGATCCCAGG
PRIMER_RIGHT_3_SEQUENCE=TACTCCACCACTTCAACCCTCT
PRIMER_LEFT_3=131,22
PRIMER_RIGHT_3=333,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=60.031
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.1076
PRIMER_LEFT_4_PENALTY=0.077340
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=AAGTGTTCTCTCCGACTTCGAC
PRIMER_RIGHT_4_SEQUENCE=CGTTCATACTCCACCACTTCAA
PRIMER_LEFT_4=190,22
PRIMER_RIGHT_4=339,22
PRIMER_LEFT_4_TM=59.923
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_03018 Ps_contig_mira-and-tgicl-ass_franssen_28207
SEQUENCE=TAGTCAACGCAGAGTACGTCGGGGAAGCAATATTAGTTTCATCTACAAAACAAGCAAAGAAAGAGAGAATGTTGAGATCCCAGGACTACCACAACCACATTGCAGGAGTCAGGAANGGGAGCCAAGTGTTCTCTCCGACTTCGACCGCCTAACCCAAACGCTAGAAATCGGCCTTACAAACGAAGGTAGGGTTGTCTACGATGGAGGTCGGAGGATCACACCGCCACCACCACCACCACCAACCNGAGATCGTGAGAGGTGA
TARGET=224,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.3653
PRIMER_LEFT_PENALTY=0.058463
PRIMER_RIGHT_PENALTY=3.306877
PRIMER_LEFT_SEQUENCE=GAGAATGTTGAGATCCCAGGAC
PRIMER_RIGHT_SEQUENCE=TCACCTCTCACGATCTCNGG
PRIMER_LEFT=64,22
PRIMER_RIGHT=261,20
PRIMER_LEFT_TM=59.942
PRIMER_RIGHT_TM=61.307
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=57.895
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=9.8000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=3.3807
PRIMER_LEFT_1_PENALTY=0.073866
PRIMER_RIGHT_1_PENALTY=3.306877
PRIMER_LEFT_1_SEQUENCE=GAGAGAATGTTGAGATCCCAGG
PRIMER_RIGHT_1_SEQUENCE=TCACCTCTCACGATCTCNGG
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=261,20
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=61.307
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=3.3842
PRIMER_LEFT_2_PENALTY=0.077340
PRIMER_RIGHT_2_PENALTY=3.306877
PRIMER_LEFT_2_SEQUENCE=AAGTGTTCTCTCCGACTTCGAC
PRIMER_RIGHT_2_SEQUENCE=TCACCTCTCACGATCTCNGG
PRIMER_LEFT_2=123,22
PRIMER_RIGHT_2=261,20
PRIMER_LEFT_2_TM=59.923
PRIMER_RIGHT_2_TM=61.307
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=57.895
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.8000
PRIMER_PAIR_2_COMPL_ANY=3.75
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=3.4445
PRIMER_LEFT_3_PENALTY=0.137575
PRIMER_RIGHT_3_PENALTY=3.306877
PRIMER_LEFT_3_SEQUENCE=GAGATCCCAGGACTACCACAAC
PRIMER_RIGHT_3_SEQUENCE=TCACCTCTCACGATCTCNGG
PRIMER_LEFT_3=73,22
PRIMER_RIGHT_3=261,20
PRIMER_LEFT_3_TM=59.862
PRIMER_RIGHT_3_TM=61.307
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=57.895
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=3.4476
PRIMER_LEFT_4_PENALTY=0.140702
PRIMER_RIGHT_4_PENALTY=3.306877
PRIMER_LEFT_4_SEQUENCE=CTAGAAATCGGCCTTACAAACG
PRIMER_RIGHT_4_SEQUENCE=TCACCTCTCACGATCTCNGG
PRIMER_LEFT_4=160,22
PRIMER_RIGHT_4=261,20
PRIMER_LEFT_4_TM=60.141
PRIMER_RIGHT_4_TM=61.307
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=57.895
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=102
=
PRIMER_SEQUENCE_ID=SSR_03019 Ps_contig_mira-and-tgicl-ass_franssen_28208
SEQUENCE=CACAAAGCACAAGCTTATGCTCTGTATGTTTTCCATTTTTGCAATCCAAGACCATTTGGCCTGTTCCTTCGTGGGTTATACTGATTGGTTTCAACATCCATGTACTCTTCATAGATCACTTCACCACCATATGGCCTAACCTCAGGTACTCGTTCATACTCCACCACTTCAACCCTCTCAACGATCTCAGGGTTGTGGTGGTGGTGGTGGCGGTGGGATCCCTCGACCTCATCGTAGACAACCCTCTTGTTGAAGGCCATTTCAGTCGTTTGGGGAGCGGTCGAAGTCGGAGAGAACACTTGGCTCCCTT
TARGET=195,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1076
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.077340
PRIMER_LEFT_SEQUENCE=CGTTCATACTCCACCACTTCAA
PRIMER_RIGHT_SEQUENCE=AAGTGTTCTCTCCGACTTCGAC
PRIMER_LEFT=150,22
PRIMER_RIGHT=300,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=59.923
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=0.1085
PRIMER_LEFT_1_PENALTY=0.031122
PRIMER_RIGHT_1_PENALTY=0.077340
PRIMER_LEFT_1_SEQUENCE=TACTCCACCACTTCAACCCTCT
PRIMER_RIGHT_1_SEQUENCE=AAGTGTTCTCTCCGACTTCGAC
PRIMER_LEFT_1=156,22
PRIMER_RIGHT_1=300,22
PRIMER_LEFT_1_TM=60.031
PRIMER_RIGHT_1_TM=59.923
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=0.2390
PRIMER_LEFT_2_PENALTY=0.237699
PRIMER_RIGHT_2_PENALTY=0.001315
PRIMER_LEFT_2_SEQUENCE=CCTGTTCCTTCGTGGGTTATAC
PRIMER_RIGHT_2_SEQUENCE=GAGGGTTGTCTACGATGAGGTC
PRIMER_LEFT_2=59,22
PRIMER_RIGHT_2=245,22
PRIMER_LEFT_2_TM=59.762
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=4.6000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=0.2563
PRIMER_LEFT_3_PENALTY=0.030276
PRIMER_RIGHT_3_PENALTY=0.225996
PRIMER_LEFT_3_SEQUENCE=CGTTCATACTCCACCACTTCAA
PRIMER_RIGHT_3_SEQUENCE=TCAACAAGAGGGTTGTCTACGA
PRIMER_LEFT_3=150,22
PRIMER_RIGHT_3=252,22
PRIMER_LEFT_3_TM=60.030
PRIMER_RIGHT_3_TM=59.774
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=0.3150
PRIMER_LEFT_4_PENALTY=0.237699
PRIMER_RIGHT_4_PENALTY=0.077340
PRIMER_LEFT_4_SEQUENCE=CCTGTTCCTTCGTGGGTTATAC
PRIMER_RIGHT_4_SEQUENCE=AAGTGTTCTCTCCGACTTCGAC
PRIMER_LEFT_4=59,22
PRIMER_RIGHT_4=300,22
PRIMER_LEFT_4_TM=59.762
PRIMER_RIGHT_4_TM=59.923
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=4.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_03020 Ps_contig_mira-and-tgicl-ass_franssen_28209
SEQUENCE=GACCATTTGGCCTGTTCCTTCGTGGGTTATACTGTTATTTGGTTTTCTAACATCCATGTACTCTTCATAGATCACTTCACCACCATATGGCCTAACCTCAGGTACTCGTTCATACTCCACCACTTCAACCCTCTCAACGATCTCAGGGTTGTGGTGGTGGTGGTGGCGGTGGGATCCCTCGACCTCATCGTAGACAACCCTCTTGTTGAAGGCCATTTCAGCGTTTGGGTAGCGGTCGAAGTCGGAGAGAACACCTTGGCTCCCTTCTGACTCCTGCAATGTGGTTGTGGTAGTCCTGGGATCTCAACATTCTCTCTTTCTTTTTGCTTGTTTTGTAAGATGAAACTAATATTGCTTCCCC
TARGET=151,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0887
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.058463
PRIMER_LEFT_SEQUENCE=CGTTCATACTCCACCACTTCAA
PRIMER_RIGHT_SEQUENCE=GAGAATGTTGAGATCCCAGGAC
PRIMER_LEFT=106,22
PRIMER_RIGHT=313,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=59.942
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.0896
PRIMER_LEFT_1_PENALTY=0.031122
PRIMER_RIGHT_1_PENALTY=0.058463
PRIMER_LEFT_1_SEQUENCE=TACTCCACCACTTCAACCCTCT
PRIMER_RIGHT_1_SEQUENCE=GAGAATGTTGAGATCCCAGGAC
PRIMER_LEFT_1=112,22
PRIMER_RIGHT_1=313,22
PRIMER_LEFT_1_TM=60.031
PRIMER_RIGHT_1_TM=59.942
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.1041
PRIMER_LEFT_2_PENALTY=0.030276
PRIMER_RIGHT_2_PENALTY=0.073866
PRIMER_LEFT_2_SEQUENCE=CGTTCATACTCCACCACTTCAA
PRIMER_RIGHT_2_SEQUENCE=GAGAGAATGTTGAGATCCCAGG
PRIMER_LEFT_2=106,22
PRIMER_RIGHT_2=315,22
PRIMER_LEFT_2_TM=60.030
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.1050
PRIMER_LEFT_3_PENALTY=0.031122
PRIMER_RIGHT_3_PENALTY=0.073866
PRIMER_LEFT_3_SEQUENCE=TACTCCACCACTTCAACCCTCT
PRIMER_RIGHT_3_SEQUENCE=GAGAGAATGTTGAGATCCCAGG
PRIMER_LEFT_3=112,22
PRIMER_RIGHT_3=315,22
PRIMER_LEFT_3_TM=60.031
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.1679
PRIMER_LEFT_4_PENALTY=0.030276
PRIMER_RIGHT_4_PENALTY=0.137575
PRIMER_LEFT_4_SEQUENCE=CGTTCATACTCCACCACTTCAA
PRIMER_RIGHT_4_SEQUENCE=GAGATCCCAGGACTACCACAAC
PRIMER_LEFT_4=106,22
PRIMER_RIGHT_4=304,22
PRIMER_LEFT_4_TM=60.030
PRIMER_RIGHT_4_TM=59.862
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_03021 Ps_contig_mira-and-tgicl-ass_franssen_28216
SEQUENCE=TTTTACGCGGGAACTCATAACAAATTTCTTCACGGTCGTTGTTACTCCTTGCTGCTACTTCATCTTTGCGTCTCTCAACCCAAGAAAAACAAACAAAGATGGCAGTCACATTGTATAACCTTAAGTCTGAATCTGGGTTGAAGAAACTTGACGAGTTCCTTCTTACACGCAGTTATATCACTGGGTACCAAGCTTCAAAGGATGATATCACTGTCTATTCAGCTTTGTCATCAGTTCCATCGAATGAATATGTCAATGTTGCTAGGTGGTACAAGCACATTGATGCTTTGTTGAGAATTTCTGGTGTTTCTGGTGAGGGATCTGGTGTCATTGTGGAATCTTCTCTTGTGGCTGAAGAGGCTATTGCCACTCCTCCAGCTGCTGACACAAAGGCCACCGAAGCAGAGGATGACGATGATGATGATGTGGATTTGTTTGGTGAAGAGACTGAGGAAGAGAAGAAGGCAGCTGAGGAACGGGCAGCAGCTGTGAAGGCATCTGGCAAAAAGAAAGAGAGTGGAAAATCATCTGTATTGTTGGATGTGAAGCCATGGGATGATGAAACCGACATGAAAAAGCTCGAAGAAGCAGTGAGATCTGTTAAGTTGGACGGATTGTTATGGGGTGCATCCAAACTTGTTCCTGTTGGGTACGGTATCAAGAAGCTTCAAATTATGATGACTATTGTGGATGACCTGGTTTCTGTCGACAATATGGTTGAAGATTATCTTACCGTCGAGCCCATCAACGAGTATGTTCAGAGTTGTGACATTGTGGCCTTCAATAAAATATAATCTGCTATTCATGTGACATGGAGATCATGATAGTGGGAGAGTATTTGCAATTATCAGAGATGACAATCGAGGGTTACTGTTATATTAGATTTTTGTTTTTTGGGAAACCTACCTTTCATTTTATGAATTTTGTCTTGTGAGTTCATCTATACTATGGATTGTGTTATAATTATTAAGTTAAGAATTGAAGTTGTGTTGTGTTTTTCGAATTTTGATTCTTTGTTGANATYTTGCTGATNCTAAAGCTTGCCAGTCTCTTTGGACTCTCTGCCACCTATAGTTTTGATGGCTTTTTACTTTAAAAGT
TARGET=414,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03022 Ps_contig_mira-and-tgicl-ass_franssen_28220
SEQUENCE=CCCTAACCGACTCCACCATTAAGTCGACAAAACCCAAAACTAAGGNAACCGCAACAGCAACCGCCGCTGCCACTTCATCCTTCATCGGAACTCGTCTCCGTGAAGTTCAATCCAATTCCGGCCGAGTACAAGCCAGATTCGGATTTGCATTCGGAAAGAAAGAAAAGACCGCAACCAAAAGAAAACCAATACAACGCTCGGACTCCAACCACCGATCGCCCGTTATGGTTTCCCGGAGCAAAGTCACCGGAGTATCTAGACGGAAGTTTGGTCGGCGATTACGGTTTCGATCCATTTGGTTTGGGGAAACCAGCTGAGTATTTGCAATTTGACCTTGATTCTCTTGATCAGAATCTGGCGAAGAATCTCGCCGGAGATGTGATCGGAACTAGGACGGAGTTTGAGGATGTTAAATCGACGCCGTTTCAACCTTACACCGAAGTTTTTGGACTTCAGAGGTTTAGAGAATGTGAATTGGTTCACGGTAGATGGGCCATGCTTGCTACTCTTGGTGCTCTTACTGTTGAATGGCTCACCGGTGTTACATGGCAAGACGCCGGAAAGGTGGNGCTAGTTGAAGATCAACATTATCTTGGACAACCACTACATTCTCATTGAACAGGTCTCCTAAACCCTCGANATATCTTATTAT
TARGET=154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3349
PRIMER_LEFT_PENALTY=0.113803
PRIMER_RIGHT_PENALTY=0.221115
PRIMER_LEFT_SEQUENCE=CCTAACCGACTCCACCATTAAG
PRIMER_RIGHT_SEQUENCE=AGATACTCCGGTGACTTTGCTC
PRIMER_LEFT=1,22
PRIMER_RIGHT=256,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=59.779
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=256
PRIMER_PAIR_PENALTY_1=0.3956
PRIMER_LEFT_1_PENALTY=0.174518
PRIMER_RIGHT_1_PENALTY=0.221115
PRIMER_LEFT_1_SEQUENCE=ACTCGTCTCCGTGAAGTTCAAT
PRIMER_RIGHT_1_SEQUENCE=AGATACTCCGGTGACTTTGCTC
PRIMER_LEFT_1=89,22
PRIMER_RIGHT_1=256,22
PRIMER_LEFT_1_TM=60.175
PRIMER_RIGHT_1_TM=59.779
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=168
PRIMER_PAIR_PENALTY_2=0.4536
PRIMER_LEFT_2_PENALTY=0.113803
PRIMER_RIGHT_2_PENALTY=0.339815
PRIMER_LEFT_2_SEQUENCE=CCTAACCGACTCCACCATTAAG
PRIMER_RIGHT_2_SEQUENCE=CCGTCTAGATACTCCGGTGACT
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=59.660
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=262
PRIMER_PAIR_PENALTY_3=0.5143
PRIMER_LEFT_3_PENALTY=0.174518
PRIMER_RIGHT_3_PENALTY=0.339815
PRIMER_LEFT_3_SEQUENCE=ACTCGTCTCCGTGAAGTTCAAT
PRIMER_RIGHT_3_SEQUENCE=CCGTCTAGATACTCCGGTGACT
PRIMER_LEFT_3=89,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=59.660
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.5191
PRIMER_LEFT_4_PENALTY=0.297952
PRIMER_RIGHT_4_PENALTY=0.221115
PRIMER_LEFT_4_SEQUENCE=AAAACTAAGGNAACCGCAACAG
PRIMER_RIGHT_4_SEQUENCE=AGATACTCCGGTGACTTTGCTC
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=256,22
PRIMER_LEFT_4_TM=60.298
PRIMER_RIGHT_4_TM=59.779
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.75
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_03023 Ps_contig_mira-and-tgicl-ass_franssen_28230
SEQUENCE=TAATAATACCGTTGGTCATCAATAGTCAAAGCACACAGTAATTTTGGGGGTAACGACCTCTGGGAGCAGGAGGAATACCAGAAAGTTCAAACTTNCCCACAAATTGTTATCCCTAGTCCTTGTTCTCTCTCCTTCAAAGACTTGGATCAACGCACCAGGCTGGTTGTCAGAATAGGTTGAGAAAACCTGTTCTTTCTTTGTTGGAATGGTGGTATTCCTTTGGATCAGAACAGTCATCACACCCCCCAGCAGTCTCCAAACCAAGAGAGAGAGGGGTAACATCAAGAAGGAGAAGATCCTGCACCTTCTCACTTCCCTCACCACTACAAATTGCAGCCTGGACNGCAGCTCCATACGCA
TARGET=264,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4245
PRIMER_LEFT_PENALTY=0.186949
PRIMER_RIGHT_PENALTY=0.237561
PRIMER_LEFT_SEQUENCE=GGTGGTATTCCTTTGGATCAGA
PRIMER_RIGHT_SEQUENCE=TGTAGTGGTGAGGGAAGTGAGA
PRIMER_LEFT=207,22
PRIMER_RIGHT=327,22
PRIMER_LEFT_TM=60.187
PRIMER_RIGHT_TM=59.762
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.4330
PRIMER_LEFT_1_PENALTY=0.195462
PRIMER_RIGHT_1_PENALTY=0.237561
PRIMER_LEFT_1_SEQUENCE=CTTNCCCACAAATTGTTATCCC
PRIMER_RIGHT_1_SEQUENCE=TGTAGTGGTGAGGGAAGTGAGA
PRIMER_LEFT_1=91,22
PRIMER_RIGHT_1=327,22
PRIMER_LEFT_1_TM=60.195
PRIMER_RIGHT_1_TM=59.762
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.3000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.75
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=0.4920
PRIMER_LEFT_2_PENALTY=0.254467
PRIMER_RIGHT_2_PENALTY=0.237561
PRIMER_LEFT_2_SEQUENCE=CTTTCTTTGTTGGAATGGTGGT
PRIMER_RIGHT_2_SEQUENCE=TGTAGTGGTGAGGGAAGTGAGA
PRIMER_LEFT_2=191,22
PRIMER_RIGHT_2=327,22
PRIMER_LEFT_2_TM=60.254
PRIMER_RIGHT_2_TM=59.762
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=0.5214
PRIMER_LEFT_3_PENALTY=0.139129
PRIMER_RIGHT_3_PENALTY=0.382228
PRIMER_LEFT_3_SEQUENCE=CCGTTGGTCATCAATAGTCAAA
PRIMER_RIGHT_3_SEQUENCE=GTGCAGGATCTTCTCCTTCTTG
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=303,22
PRIMER_LEFT_3_TM=59.861
PRIMER_RIGHT_3_TM=60.382
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.5264
PRIMER_LEFT_4_PENALTY=0.288804
PRIMER_RIGHT_4_PENALTY=0.237561
PRIMER_LEFT_4_SEQUENCE=TTCCTTTGGATCAGAACAGTCA
PRIMER_RIGHT_4_SEQUENCE=TGTAGTGGTGAGGGAAGTGAGA
PRIMER_LEFT_4=214,22
PRIMER_RIGHT_4=327,22
PRIMER_LEFT_4_TM=59.711
PRIMER_RIGHT_4_TM=59.762
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=114
=
PRIMER_SEQUENCE_ID=SSR_03024 Ps_contig_mira-and-tgicl-ass_franssen_28233
SEQUENCE=CGGCCAGTAAAAATTGCTTCCTTCCATTATCAAAAATAATTCGTAACACCCAAAATTAAAGACAAAAACCATAGTCATGACAATGTCTCATGTCAGAAAGGTAACCCAAAAACGCTGACCAATAAATATTCTAAAACAAGATTATAAAAATCAAAAGGGTCTTGAACAAAATACCCAATCTGCATCAAATCAATAACTTAGTCAACTTCCTCAATTTTGGGACCAGCACCACCTGCAGCTGGAGGAACTTCATCATCATCACCACCCATGTCCGGAGCTCCTCCACCTTGATACATCTTAGCAATGATTGGATTGCAAAGACTTTCAAGCTCCTTCATCTTATCTTCAAACTCATCTGCTTCAGCCAATTGGTTACTATCCAACCACTGAATTGCAGCCTCAATTGCATCATCTATCTTCTTCTTGTCTTCAGGGGAGAGCTTTGATCCAATTTTTTCATCATTGATTGTGTTCCTCATGTTGTAAGCATAGTTTTCCAAAGCATTCTTTGCATCAACCTTCTTCTTGTGCTCCTCATCTTCTGCCTTGTATTTCTCTGCTTCCTCAACCATCTTCTCAATCTCCTCCTTCGAGAGTCTACCTTTGTCGTTAGTAATTGTGATCTTGTTCTTTTGCCCAGTTGTTTTGTCCTCAGCCGAGACATTCAAGATACCATTGGCATCAATGTCAAAGCAAACAGTGATTTGAGGTACACCACGGGGTGCTGGAGGAATTCCAGAAAGCTCAAATTTACCCAACAAGTTGTTGTCTTTGGTTCTTGCTCTTTCACCCTCAAAAACTTGGATCAAGACACCAGGTTGGTTATCTGAGTAAGTAGAGAATACTTGTTCCTTCTTGGTTGGAATTGTAGTATTCCTTTGAATAAGAACAGTCATGACACCACCAGCAGTTTCTAGACCTAGAGACAATGGAGTGACGTCCAAAAGCAACAAATCTTGTACCTTCTCATTACCTTCACCACTCAAGATGGCAGCTTGGACAGCAGCNCCATAAGCAACAGCCTCGTCAGGGTTGATACTCTTGCATAGCTCCTTACCATTGAAGAAGTCCTGCAACAACTGCTGAACTTTAGGAATCCTAGTGGAACCACCAACAAGAACAACATCATGAACTGTGCTCTTGTCCATCTTTGCATCCCTAAGACACTTCTCCACAGGCTCCATACACTTCCTGAACAAATCCATGTTCAGCTCCTCAAACCTAGCACGTGTGATTGTGGTGTAAAAATCAACACCCTCATAAAGGGAATCAATCTCAATAGTTGTCTGTGCAGTTGATGAGAGAGTCCTCTTTGCTCTCTCACAAGCAGTTCTCAACCTCCTAAGTGCCCTAGGGTTTCCACTAATATCCTTCTTATTCTTCCTCTTAAACTCTTGAACAAAATGATTAACCATCCTGTTATCAAAATCTTCACCACCAAGATGGGTATCACCAGCAGTGGCTTTAACTTCAAAAATACCCTCTTCAATAGTAAGAAGAGACACATCAAAAGTACCACCTCCCAAATCAAAGATCAACACATTCTTCTCACCAACACTTGTAGCTTTCTTGTCAAGCCCGTATGCAATAGCAGCTGCAGTTGGCTCATTGATTATTCTCAGGACATTCAAGCCAGCAATAGCACCAGCATCCTTTGTAGCTTGACGCTGGGAGTCATTGAAGTAAGCAGGAACGGTAACAACAGCGTTCTTCACAGTAGAACCAAGATAAGCCTCAGCAATCTCACGCATCTTAATAAGCACCATAGAGGAAATTTCCTCGGCAGAAAATTGTTTGTCCTCACCCTTA
TARGET=250,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1011
PRIMER_LEFT_PENALTY=0.088662
PRIMER_RIGHT_PENALTY=0.012411
PRIMER_LEFT_SEQUENCE=GGGTCTTGAACAAAATACCCAA
PRIMER_RIGHT_SEQUENCE=CTCTCCCCTGAAGACAAGAAGA
PRIMER_LEFT=156,22
PRIMER_RIGHT=439,22
PRIMER_LEFT_TM=60.089
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.2713
PRIMER_LEFT_1_PENALTY=0.258908
PRIMER_RIGHT_1_PENALTY=0.012411
PRIMER_LEFT_1_SEQUENCE=AGGGTCTTGAACAAAATACCCA
PRIMER_RIGHT_1_SEQUENCE=CTCTCCCCTGAAGACAAGAAGA
PRIMER_LEFT_1=155,22
PRIMER_RIGHT_1=439,22
PRIMER_LEFT_1_TM=59.741
PRIMER_RIGHT_1_TM=59.988
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.3093
PRIMER_LEFT_2_PENALTY=0.088662
PRIMER_RIGHT_2_PENALTY=0.220659
PRIMER_LEFT_2_SEQUENCE=GGGTCTTGAACAAAATACCCAA
PRIMER_RIGHT_2_SEQUENCE=TAGTAACCAATTGGCTGAAGCA
PRIMER_LEFT_2=156,22
PRIMER_RIGHT_2=377,22
PRIMER_LEFT_2_TM=60.089
PRIMER_RIGHT_2_TM=59.779
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.3582
PRIMER_LEFT_3_PENALTY=0.088662
PRIMER_RIGHT_3_PENALTY=0.269585
PRIMER_LEFT_3_SEQUENCE=GGGTCTTGAACAAAATACCCAA
PRIMER_RIGHT_3_SEQUENCE=AATTGGCTGAAGCAGATGAGTT
PRIMER_LEFT_3=156,22
PRIMER_RIGHT_3=369,22
PRIMER_LEFT_3_TM=60.089
PRIMER_RIGHT_3_TM=60.270
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.3582
PRIMER_LEFT_4_PENALTY=0.088662
PRIMER_RIGHT_4_PENALTY=0.269585
PRIMER_LEFT_4_SEQUENCE=GGGTCTTGAACAAAATACCCAA
PRIMER_RIGHT_4_SEQUENCE=ATTGGCTGAAGCAGATGAGTTT
PRIMER_LEFT_4=156,22
PRIMER_RIGHT_4=368,22
PRIMER_LEFT_4_TM=60.089
PRIMER_RIGHT_4_TM=60.270
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_03025 Ps_contig_mira-and-tgicl-ass_franssen_28236
SEQUENCE=GTCGGGGCCTTTCATTTCTAATCACAGTTTCAGGCTGTGATTTCCACCCTCTTCCTCTCAGATCCCTCTCTTTTGCGTAACGAACCAAAAGAGTGAAATCCAATGGCCGGAAAGGGAGAGGGACCGGCTATCGGAATCGATCTCGGAACTACATACTCCTGCGTCGGTGTGTGGCAACATGACAGAGTTGAAATCATCGCCAATGACCAAGGAAACAGAACCACGCCGTCGTACGTCGCTTTCACCGACTCCGAACGTCTCATCGGTGATGCCGCTAAGAATCAAGTCGCCATGAACCCTATCAACACCGTTTTTGATGCCAAGAGGTTGATTGGTCGTAGAGTCTCTGATGCATCTGTTCAGAGTGACATGAAGTTGTGGCCTTTTAAGATTATTGCTGGTCCAGCTGAAAAGCCAATGATTGTTGTTAACTACAAGGGTGAAGATAAGCAATTTGCAGCTGAAGAGATCTCTTCAATGGTTCTGATGAAGATGCGTGAGATTGCTGAAGCTTACCTTGGCTCTGCAATTAAGAATGCTGTTGTTACTGTCCCTGCTTACTTTAATGATTCCCAGCGTCAAGCTACTAAGGATGCTGGCGTGATTGCTGGTCTTAATGTCATGCGAATCATCAATGAGCCCACTGCTGCTGCCATTGCATATGGGTTAGACAAGAAAGCAACAAGTGTTGGTGAAAAGAATGTCTTGATTTTTGACCTTGGTGGTGGTACTTTTGATGTCTCTTTGCTTACTATTGAAGAGGGTATCTTTGAGGTGAAAGCCACAGCTGGAGACACCCATCTTGGAGGTGAGGATTTTGATAACAGAATGGTGAACCACTTTGTTCAAGAGTTCAAGAGAAAGAACAAGAAGGACATTAGTGGCAACCCTAGGGCACTCAGGCGATTGAGGACTGCATGTGAGAGGGCGAAGAGAACTCTTTCATCTACTGCCCAGACTACCATTGAAATTGACTCTCTATTTGAGGGAATTGATTTCTACTCCCCTATTACTCGTGCCAGATTTGAGGAGCTCAACATGGATCTCTTTAGGAAATGTATGGAACCAGTTGAGAAATGTTTGAGAGATGCCAAGATGGAACAAAAAAAGCATCCATGATGTTGTTCTTGTTGGTGGTTCTACTAGAATTCCAAAGGTTCAACAACTTTTGCAGGATTTCTTTAATGGAAAGGAGCTATGCAAAAGCATTAATCCCGATGAGGCTGTTGCGTATGGAGCTGCTGTCCAGGCTGCAATTTTGAGTGGTGAGGGGAATGAGAAGGTGCAGGATCTTCTCCTTCTTGATGTTACCCCTCTCTCTCTTGGTTTGGAGACTGCTGGGGGTGTGATGACTGTTCTGATCCAAAGGAATACCACCATTCCAACAAAGAAAGAACAGGTTTTCTCAACCTATTCTGACAACCAGCCTGGTGTCTTGATCCAAGTCTTTGAAGGAGAGAGAACAAGGACTAGGGATAACAATTTGTTGGGCAAGTTTGAACTTTCTGGTATTCCTCCTGCTCCCAGAGGTGTTCCCCAAATTACCGTGTGCTTTGACATTGATGCCAATGGTATATTGAATGTCTCTGCTGAAGACAAGACTACCGGGCAGAAGAACAAGATCACCATCACAAATGACAAGGGTAGGCTTTCAAAGGATGATATTGAGAAGATGGTTCAGGAGGCTGAGAAATACAAGTCTGAGGATGAAGAGCATAAGAAGAAGGTTGAGGCCAAGAATGCATTAGAAAACTATGCATACAACATGAGGAACACCATTAAGGATGATAAGATTGCAGGTAAACTTGATCCAGCTGACAAAAAGAAGATCGAGGATGCCATTGAACAGGCTATCCAGTGGCTTGACAGCAACCAGCTTGCTGAGGCCGATGAATTTGAGGACAAGATGAAGGAACTTGAAGGCCTCTGCAACCCTATCATTGCCAAGATGTATCAAGGTGGTGCAGGTCCAGAGGGTGCTGCTCCAACTGATGAAGAATATGCTCCTTCTGGTGGAAGTGGTGCTGGTCCCAAGATTGAGGAAGTCGACTAAATGTAGTTGCTTTTTATTTCTTATCTATGGTTTATTTTTCATGTTGTATTTTTGAATTGGTGTATTCATTGATGGATTTAGCCAGAGTGCTAATTTTTAGTGTATGTCATAACATTCGTTTGGTACTGTTAAGCCCCCATTTTGTAGTTTTTATTCATTGTAAGAATGATACTTTTATATTTATACGATGTTTTTCAAAAAAGT
TARGET=1314,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1870
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.186949
PRIMER_LEFT_SEQUENCE=AGCATCCATGATGTTGTTCTTG
PRIMER_RIGHT_SEQUENCE=GGTGGTATTCCTTTGGATCAGA
PRIMER_LEFT=1108,22
PRIMER_RIGHT=1377,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.187
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=270
PRIMER_PAIR_PENALTY_1=0.2545
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.254467
PRIMER_LEFT_1_SEQUENCE=AGCATCCATGATGTTGTTCTTG
PRIMER_RIGHT_1_SEQUENCE=CTTTCTTTGTTGGAATGGTGGT
PRIMER_LEFT_1=1108,22
PRIMER_RIGHT_1=1393,22
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=60.254
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.3213
PRIMER_LEFT_2_PENALTY=0.134318
PRIMER_RIGHT_2_PENALTY=0.186949
PRIMER_LEFT_2_SEQUENCE=GCATCCATGATGTTGTTCTTGT
PRIMER_RIGHT_2_SEQUENCE=GGTGGTATTCCTTTGGATCAGA
PRIMER_LEFT_2=1109,22
PRIMER_RIGHT_2=1377,22
PRIMER_LEFT_2_TM=59.866
PRIMER_RIGHT_2_TM=60.187
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=0.3888
PRIMER_LEFT_3_PENALTY=0.134318
PRIMER_RIGHT_3_PENALTY=0.254467
PRIMER_LEFT_3_SEQUENCE=GCATCCATGATGTTGTTCTTGT
PRIMER_RIGHT_3_SEQUENCE=CTTTCTTTGTTGGAATGGTGGT
PRIMER_LEFT_3=1109,22
PRIMER_RIGHT_3=1393,22
PRIMER_LEFT_3_TM=59.866
PRIMER_RIGHT_3_TM=60.254
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.4297
PRIMER_LEFT_4_PENALTY=0.242742
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=ATGATGTTGTTCTTGTTGGTGG
PRIMER_RIGHT_4_SEQUENCE=GGTGGTATTCCTTTGGATCAGA
PRIMER_LEFT_4=1115,22
PRIMER_RIGHT_4=1377,22
PRIMER_LEFT_4_TM=59.757
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_03026 Ps_contig_mira-and-tgicl-ass_franssen_28246
SEQUENCE=TTAGTTAGAAAGAAAATATTTATTCTATTTTTTGAATTTGTAATACACTGAAACTAAACAACTGATTAGTCAACAAGCAAAACGAAAATGAGAAATTACAAGAAAACTCTGAAATCATACATCTAGTTATTTTCATACTACGCCTCAAGCGACTCTTTTTTTATTTACAACACCACTTGCCACCAATCTACCTAGGCTGATGAATAGCCCGTGGGCTCACTCTGGGCTTAAAATTCTTCGGAGCCTTCTCCGCATATTTCGGAGCTTCAGCCCACCCACCAGATCCTCTCCATTTCAACGATCCCAACGAGACCAAATCTTTCCCATCTTCGTGTTGGTGTTCAATTTGATGGACATGGAAAAGATCGTCTTCATCCATATCAAAATCCGAAAACGCGTCGTTTTGATTCTGTGGATCTTGGAAGCGTTCGCGGAGCCAGTAATCGCGAAACCAAGGGGAGGAGTGAACTAGGGTCCACCAGTCGTCGGAGAAATCTTCCACCGTGCGATAAGCTAGTGGAACGAACATCGGAGCGTTGGGATTCAACGAGGAGGAGGTGGTTCTTGTTGAAGAGAGTACTTCCATTGATGTGGTGGAAAACAAGATAAGGATAACGTAGTGATGGAGAGAGAGAGTGAAAAGAGTAAAGTGTGTAACCAGTGTTGCTGAAAGGTTGGATTGTTGATCCAGCAACAATATCATCCCAAAGTTTATCTAACATTTTGTTTGTTTGATAAAAGAGTTTGATC
TARGET=626,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1856
PRIMER_LEFT_PENALTY=0.145769
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=GAGGAGGTGGTTCTTGTTGAAG
PRIMER_RIGHT_SEQUENCE=AATCCAACCTTTCAGCAACACT
PRIMER_LEFT=551,22
PRIMER_RIGHT=680,22
PRIMER_LEFT_TM=60.146
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=0.2672
PRIMER_LEFT_1_PENALTY=0.227356
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=TACTTCCATTGATGTGGTGGAA
PRIMER_RIGHT_1_SEQUENCE=AATCCAACCTTTCAGCAACACT
PRIMER_LEFT_1=577,22
PRIMER_RIGHT_1=680,22
PRIMER_LEFT_1_TM=60.227
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=104
PRIMER_PAIR_PENALTY_2=0.3227
PRIMER_LEFT_2_PENALTY=0.282868
PRIMER_RIGHT_2_PENALTY=0.039879
PRIMER_LEFT_2_SEQUENCE=GTACTTCCATTGATGTGGTGGA
PRIMER_RIGHT_2_SEQUENCE=AATCCAACCTTTCAGCAACACT
PRIMER_LEFT_2=576,22
PRIMER_RIGHT_2=680,22
PRIMER_LEFT_2_TM=59.717
PRIMER_RIGHT_2_TM=60.040
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=0.3235
PRIMER_LEFT_3_PENALTY=0.283593
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=GCGATAAGCTAGTGGAACGAAC
PRIMER_RIGHT_3_SEQUENCE=AATCCAACCTTTCAGCAACACT
PRIMER_LEFT_3=505,22
PRIMER_RIGHT_3=680,22
PRIMER_LEFT_3_TM=60.284
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=0.3491
PRIMER_LEFT_4_PENALTY=0.145769
PRIMER_RIGHT_4_PENALTY=0.203343
PRIMER_LEFT_4_SEQUENCE=GAGGAGGTGGTTCTTGTTGAAG
PRIMER_RIGHT_4_SEQUENCE=CCTTTCAGCAACACTGGTTACA
PRIMER_LEFT_4=551,22
PRIMER_RIGHT_4=673,22
PRIMER_LEFT_4_TM=60.146
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_03027 Ps_contig_mira-and-tgicl-ass_franssen_28246
SEQUENCE=TTAGTTAGAAAGAAAATATTTATTCTATTTTTTGAATTTGTAATACACTGAAACTAAACAACTGATTAGTCAACAAGCAAAACGAAAATGAGAAATTACAAGAAAACTCTGAAATCATACATCTAGTTATTTTCATACTACGCCTCAAGCGACTCTTTTTTTATTTACAACACCACTTGCCACCAATCTACCTAGGCTGATGAATAGCCCGTGGGCTCACTCTGGGCTTAAAATTCTTCGGAGCCTTCTCCGCATATTTCGGAGCTTCAGCCCACCCACCAGATCCTCTCCATTTCAACGATCCCAACGAGACCAAATCTTTCCCATCTTCGTGTTGGTGTTCAATTTGATGGACATGGAAAAGATCGTCTTCATCCATATCAAAATCCGAAAACGCGTCGTTTTGATTCTGTGGATCTTGGAAGCGTTCGCGGAGCCAGTAATCGCGAAACCAAGGGGAGGAGTGAACTAGGGTCCACCAGTCGTCGGAGAAATCTTCCACCGTGCGATAAGCTAGTGGAACGAACATCGGAGCGTTGGGATTCAACGAGGAGGAGGTGGTTCTTGTTGAAGAGAGTACTTCCATTGATGTGGTGGAAAACAAGATAAGGATAACGTAGTGATGGAGAGAGAGAGTGAAAAGAGTAAAGTGTGTAACCAGTGTTGCTGAAAGGTTGGATTGTTGATCCAGCAACAATATCATCCCAAAGTTTATCTAACATTTTGTTTGTTTGATAAAAGAGTTTGATC
TARGET=723,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03028 Ps_contig_mira-and-tgicl-ass_franssen_28249
SEQUENCE=GTCGGGGATCAAACTCTTTTATCAAACAAACAAAATGTTAGATAAACTTTGGGATGATATTGTTGCTGGCCCTCAACCAGAACGTGGCCTTGAAAAGCTTAGAAAACTCACCACGCNTCTTCTAAATTCTAAAACCAACTTTTCCCTCAGCCGCCCTTGCTTTCCATTCGATCTCACCTCTTACTTACTTCCTTTTCCCTTTCCCATCTCATCGGTGCTAGCGATGTCGACTCCGGCTAGGAAGAGACTGATGAGAGATTTTAAGAGGCTGCAACAAGATCCACCTGCAGGATCAGTGGTGCGCCTCAGGAATAACAACATCATGCTGGTGGAATGCTGTCATTAGTTTTCGGTCCTGATAGACTACCTCCAGGTGGGGAGTTGGGACGGTCTTTAAACGG
TARGET=23,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.8895
PRIMER_LEFT_PENALTY=2.825301
PRIMER_RIGHT_PENALTY=0.064245
PRIMER_LEFT_SEQUENCE=GTCGGGGATCAAACTCTTTTA
PRIMER_RIGHT_SEQUENCE=GAATTTAGAAGANGCGTGGTGA
PRIMER_LEFT=0,21
PRIMER_RIGHT=128,22
PRIMER_LEFT_TM=58.175
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=129
PRIMER_PAIR_PENALTY_1=2.8998
PRIMER_LEFT_1_PENALTY=2.825301
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=GTCGGGGATCAAACTCTTTTA
PRIMER_RIGHT_1_SEQUENCE=AAGAGGTGAGATCGAATGGAAA
PRIMER_LEFT_1=0,21
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=58.175
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=2.9268
PRIMER_LEFT_2_PENALTY=2.825301
PRIMER_RIGHT_2_PENALTY=0.101479
PRIMER_LEFT_2_SEQUENCE=GTCGGGGATCAAACTCTTTTA
PRIMER_RIGHT_2_SEQUENCE=TTTCTAAGCTTTTCAAGGCCAC
PRIMER_LEFT_2=0,21
PRIMER_RIGHT_2=104,22
PRIMER_LEFT_2_TM=58.175
PRIMER_RIGHT_2_TM=59.899
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=2.9983
PRIMER_LEFT_3_PENALTY=2.825301
PRIMER_RIGHT_3_PENALTY=0.173020
PRIMER_LEFT_3_SEQUENCE=GTCGGGGATCAAACTCTTTTA
PRIMER_RIGHT_3_SEQUENCE=ATGGGAAAGGGAAAAGGAAGTA
PRIMER_LEFT_3=0,21
PRIMER_RIGHT_3=206,22
PRIMER_LEFT_3_TM=58.175
PRIMER_RIGHT_3_TM=60.173
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=3.1125
PRIMER_LEFT_4_PENALTY=2.825301
PRIMER_RIGHT_4_PENALTY=0.287182
PRIMER_LEFT_4_SEQUENCE=GTCGGGGATCAAACTCTTTTA
PRIMER_RIGHT_4_SEQUENCE=AGAGGTGAGATCGAATGGAAAG
PRIMER_LEFT_4=0,21
PRIMER_RIGHT_4=180,22
PRIMER_LEFT_4_TM=58.175
PRIMER_RIGHT_4_TM=59.713
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_03029 Ps_contig_mira-and-tgicl-ass_franssen_28250
SEQUENCE=AAGCAGTGGTATCAACGCAGAGTACGCGGGGATCAAACTCTTTTATCAAACAAACAAAATGTTAGATAAACTTTGGGATGATATTGTTGCTGGCCCTCAACCAGAACGTGGCCTTGAAAAGCTTAGAAAACTCACCACCACCCTCAAAGATGATGGAGCGAGTAATCAATTAATGAGAAGCACGTCGATTCCGACAACACCGACGACACCGGTGACTCCGACAACACCTTCATCGGCGCGTAAAGTTGACAACGTTTGGAGGAGCGTGTTCAATCCTGGGAGTAACTCTGCTACAAAGTCAATTGGTGCTCATGTTTTCGACAAACCACTCCCCAACACCCCCACTGTCTATGATTGGATGTACAGTGGGGATACGAGAAGCAAGCACCGTTGATTAGTGATCAAGTGGATACCAACTCCCTTGGGTTTTGCATGTAAATAGTGTTCTGTTTTGTTCATCTTCTGCCACGTGTCTACATCACCGTTACGTGTTGAGATCTGATTGGTTGTTGCAGAGTTGTTTTTTTTTATTAAGCATCTAATCTGAATCTGAATGTAAAATACTTAATAAATTGAATTAGTATGCTTTAATGAGTTTGTATTTAACTTTTATCTGAATGGATTAGGACTGTATTAATAATAATYTTAGGTGAGATGCATACAATACAATTAGAATCCAACTAAATTGATTTATATCCAATTTNNNAANCNTTCTAGTAATTTAATACTACTTGGAAATAATTAAATTTGGGCACATACAGTCCTTTATAA
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03030 Ps_contig_mira-and-tgicl-ass_franssen_28251
SEQUENCE=TTAGATAGAGAAAGAAAATGTTTATTCTATTTTTTGTAATTTGTAATACACTGAAACTAAACAACTGATTAGTCAACAAACAAAACGAAAATGAGAAATTACAAGAAAACTCTGAAATCATACATCTAGTTATTTTCATACTACGCCTCAAGCGACTCTTTTTTTATTTACAACACCACTTGCCACCAATCTACCTAGGCTGATGAATAGCCCGTGGGCTCACTCTGGGCTTAAAATTCTTCGGAGCCTTCTCCGCATATTTCGGAGCTTCAGCCCACCCACCAGATCCTCTCCATTTCAACGATCCCAACGAGACCAAATCTTTCCCATCTTCGTGTTGGTGTTCAATTTGATGGACATGGAAAAGATCGTCTTCATCCATATCAAAATCCGAAAATGCGTCGTTTTGATTCTGTGGATCTTGGAAGCGTTCGCGGAGCCAGTAATCGCGAAACCAAGGGGAGGAGTGAACTAGGGTCCACCAGTCGTCGGAGAAATCTTCCACCGTGCGATAAGCTAGTGGAACGAACATCGGAGCATTGGGATTCAACGAGGAGGAGGTGGTTCTTGTTGAAGAGAGTACTTCCATTGATGTGGTGGAAAACAAGATAAGGATAACGCAGTGATGGAGAGAGAGAGAGTGAAAAGAGTAAAGTGTGTAACCAGTGTTGTTGAAAGGTTGGATTGTTGATCCCCGAACNGNANGNAGATNAAGTGNTAGGTTCCTGCAGAAATTCCTTTGAAGAACTTCTGCTAAACAAAAACGAAGAAGAGCAAGAAAGAACTTACTAAAACT
TARGET=629,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3565
PRIMER_LEFT_PENALTY=0.227356
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=TACTTCCATTGATGTGGTGGAA
PRIMER_RIGHT_SEQUENCE=GGAATTTCTGCAGGAACCTANC
PRIMER_LEFT=580,22
PRIMER_RIGHT=737,22
PRIMER_LEFT_TM=60.227
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.75
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.3500
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.75
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=0.3915
PRIMER_LEFT_1_PENALTY=0.262407
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=CATTGATGTGGTGGAAAACAAG
PRIMER_RIGHT_1_SEQUENCE=GGAATTTCTGCAGGAACCTANC
PRIMER_LEFT_1=586,22
PRIMER_RIGHT_1=737,22
PRIMER_LEFT_1_TM=60.262
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.75
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.3500
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.4120
PRIMER_LEFT_2_PENALTY=0.282868
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=GTACTTCCATTGATGTGGTGGA
PRIMER_RIGHT_2_SEQUENCE=GGAATTTCTGCAGGAACCTANC
PRIMER_LEFT_2=579,22
PRIMER_RIGHT_2=737,22
PRIMER_LEFT_2_TM=59.717
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.75
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.3500
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.4127
PRIMER_LEFT_3_PENALTY=0.283593
PRIMER_RIGHT_3_PENALTY=0.129103
PRIMER_LEFT_3_SEQUENCE=GCGATAAGCTAGTGGAACGAAC
PRIMER_RIGHT_3_SEQUENCE=GGAATTTCTGCAGGAACCTANC
PRIMER_LEFT_3=508,22
PRIMER_RIGHT_3=737,22
PRIMER_LEFT_3_TM=60.284
PRIMER_RIGHT_3_TM=60.129
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.75
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.3500
PRIMER_PAIR_3_COMPL_ANY=3.75
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=0.7048
PRIMER_LEFT_4_PENALTY=0.575721
PRIMER_RIGHT_4_PENALTY=0.129103
PRIMER_LEFT_4_SEQUENCE=CGATAAGCTAGTGGAACGAACA
PRIMER_RIGHT_4_SEQUENCE=GGAATTTCTGCAGGAACCTANC
PRIMER_LEFT_4=509,22
PRIMER_RIGHT_4=737,22
PRIMER_LEFT_4_TM=59.424
PRIMER_RIGHT_4_TM=60.129
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.75
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.3500
PRIMER_PAIR_4_COMPL_ANY=3.75
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_03031 Ps_contig_mira-and-tgicl-ass_franssen_28252
SEQUENCE=GGTAACCAAAATAACACTTGATTCAATTTTAACCCTAGATATTTTAAGCATCACTCTCTCTATATNTAACTAACAYTCTTATTACANATNTCTCATCAAACTCTTTTATCAAACAAACAAAATGTTAGATAAACTTTGGGATGATATTGTTGCTGGCCCTCAACCAGAACGTGGCCTTGAAAAGCTTAGAAAACTCACCACCACCCTCAAAGATGATGGAGCGAGTAATCAATTAATGAGAAGCACGTCGATTCCGACAACACCGACGACACCGGTGACTCCGACAACACCTTCATCGGCGCGTAAAGTTGACAACGGTTTGGAGGAGCGTGTTCAATCCGTGGGAA
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03032 Ps_contig_mira-and-tgicl-ass_franssen_28257
SEQUENCE=GATAAGAAATTTACCTATGTCAAAAGAAGAACCTTTAGTGAATACATTCATTCATTCAGACCGATTCGCGACCTCTCACATTATGATNAGTCAAGTTAAAGCCCCAACCATAGCTTCACCCAATCCACAACTAACCTCGGCCAAACCTCAGGATCACTGTAATGAGTAACAGCCTGAACAATCGCTCGAGCCCGTTTCGCCGGGTCGCCGCTCTTGAAAACACCAGAACCAACAAACACACCATCACAACCAAGCTGCATCATCAACGCTGCATCAGCCGGTGTAGCGACACCACCGGCGGACAAACTGAAACAACCGGAAGCCCTCCCGAGTTCGCCCTTCGTCTGCAATAACAAGATCAAGTAAGGANCAGCTAATTTTCGTAGGAAG
TARGET=45,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03033 Ps_contig_mira-and-tgicl-ass_franssen_28258
SEQUENCE=GCACCAAAAGGGAAATGGCTTGAAACACAATCCAATTGAGCAAAGATTAACATCCAATATTAACTTATTAAAAAAGACAATAGATCCAACATAACAATAGAATTCAATACTCAAACAAGAATGGTTCTAGTTCATGATGAATCCACAGACTCAACATGACAATAGAATTCAATACTCAAACAAGAATGGTTCTAGTTCATGATGAATCCACAGACTCAACATGACAATAGAATTCAATACTCAAACAAGAATGGTTCTAGTTCATGATGAATCCACAGACTCAACATACTAGTTTAAACACTATAATAACCTTGGATGTCTAAAACAAGTATCAGCATATTACAAGCCACGGAAGGCTGGCTTCTTCTTTTCTTTTTTTCTTTTGTTTTCTTTCTTCCATCTAAGACAGTTCAAAAACATAAGAAGCAAAAACATGATAACCTTATTAAAATCAAATATAAGAAACAACTAAAAAAACTAGTAAAATAAGATAAGATAAAACCCTTTCAGATAGAAATTTCCATGCAAAAGAGAACCTTTAGTGAATCCATTCATTCATTCAGACCGATTCGCGAACCTCTCGACATTATGATCAGTCAAGTTAAGCCCAACCATAGCTTCACCCAATCCACAACTAACCTCGGCCAAAACCTCAGGATCACTGTAATGAGTAACAGCCTGAACAATCGCTCGAGCCCGTTTCGCCGGGTCGCCGCTCTTGAAAACACCAGAACCAACAAACACACCATCACAACCAAGCTGCATCATCAACGCTGCATCAGCCGGTGTAGCAACACCACCGGCGGCAAACTGAACAACCGGAAGCCTCCCGAGTTGCTTCGTCTGCATAACGAGATCATAAGGAGCAGCAATTTTCTTGGAGAAAGTGAAAACCTCGTCGTCATCCATGTTTGCAAGAACCCTAATATCACCGAGAACGGACCTAACATGTCTAACAGCTTCAATGATGTTACCGGTCCCGGCTTCACCTTTGGTACGAATCATGGCAGCTCCCTCTCTGATACGACGGAGAGCTTCACCGAGGTTCCGGCAACCGCAAACGAAAGGGATACGGAAGTTATGTTTGTTGATGTGATTATCTTCATCAGCTAGGGTTAGAACCTCACTCTCATCGACGTAATCGATTCCGATAGCTTCGAGGATCTGAGCTTCAACGAAGTGACCGATACGGGCCTTGGCCATGACGGGGATAGTGACGGCTTGTTTGATTTCTTTGATGAGCTGAGGGTCGCTCATACGAGCGACGCCGCCTTGGGCGCGGATGTCGGCGGGGACACGTTCGAGAGCCATGACGGCGCAGGCACCGGCTTCTTCAGCGATGCGGGCTTGTTCGGCGTTGACAACGTCCATAATGACGCCGCCGCGGAGCATTTGGGCGAGGCCGACTTTGACGGAGAAAGGAGAGGATTTCTTGGTTGTTTCGGTGAGGGCACCGTTGCCGTAGACAGTAACAACTCCGGATCCTTCCATTAGGAGAGAGATGAAATGAAAATGGTGTCTGGAAATGATGAAGGCGGAGGAGATTGAGGATCAGGAAGGGAGAGGCGATGAGAGGTTTATGTAGTGCTCAAATCTATCAATTAATATCTACCTTCACTACCCGAATCATATCCAGCCACGCATTTATTTTTACTAGTGTT
TARGET=549,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2733
PRIMER_LEFT_PENALTY=0.024252
PRIMER_RIGHT_PENALTY=0.249074
PRIMER_LEFT_SEQUENCE=AGGCTGGCTTCTTCTTTTCTTT
PRIMER_RIGHT_SEQUENCE=GTTGTGGATTGGGTGAAGCTAT
PRIMER_LEFT=353,22
PRIMER_RIGHT=634,22
PRIMER_LEFT_TM=60.024
PRIMER_RIGHT_TM=60.249
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.2733
PRIMER_LEFT_1_PENALTY=0.024252
PRIMER_RIGHT_1_PENALTY=0.249074
PRIMER_LEFT_1_SEQUENCE=AAGGCTGGCTTCTTCTTTTCTT
PRIMER_RIGHT_1_SEQUENCE=GTTGTGGATTGGGTGAAGCTAT
PRIMER_LEFT_1=352,22
PRIMER_RIGHT_1=634,22
PRIMER_LEFT_1_TM=60.024
PRIMER_RIGHT_1_TM=60.249
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.3368
PRIMER_LEFT_2_PENALTY=0.024252
PRIMER_RIGHT_2_PENALTY=0.312541
PRIMER_LEFT_2_SEQUENCE=AGGCTGGCTTCTTCTTTTCTTT
PRIMER_RIGHT_2_SEQUENCE=AGCTATGGTTGGGCTTAACTTG
PRIMER_LEFT_2=353,22
PRIMER_RIGHT_2=618,22
PRIMER_LEFT_2_TM=60.024
PRIMER_RIGHT_2_TM=59.687
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.3368
PRIMER_LEFT_3_PENALTY=0.024252
PRIMER_RIGHT_3_PENALTY=0.312541
PRIMER_LEFT_3_SEQUENCE=AAGGCTGGCTTCTTCTTTTCTT
PRIMER_RIGHT_3_SEQUENCE=AGCTATGGTTGGGCTTAACTTG
PRIMER_LEFT_3=352,22
PRIMER_RIGHT_3=618,22
PRIMER_LEFT_3_TM=60.024
PRIMER_RIGHT_3_TM=59.687
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.4288
PRIMER_LEFT_4_PENALTY=0.024252
PRIMER_RIGHT_4_PENALTY=0.404566
PRIMER_LEFT_4_SEQUENCE=AGGCTGGCTTCTTCTTTTCTTT
PRIMER_RIGHT_4_SEQUENCE=ATGGTTGGGCTTAACTTGACTG
PRIMER_LEFT_4=353,22
PRIMER_RIGHT_4=614,22
PRIMER_LEFT_4_TM=60.024
PRIMER_RIGHT_4_TM=60.405
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_03034 Ps_contig_mira-and-tgicl-ass_franssen_28275
SEQUENCE=GTATACAACGCAGAGTACGCGGGGNNACTATACTTCATACTACATAATAATACTAACATTTAACTAAGAGTTGGCTTCTCTGCAAAACTTCAATACTACAAACTTCACCTTCAAAATACTCAGTGTTCCTTGTTGGCTCAATCATGGCTTCGGCTACTTTCTCTGTAGCCAAACCAGCTATTAAGGCAAATGGGAAAGGCTTCTCTGAATTCTCTGGTCTCCGCAACTCTTCAAGACATCTTCCCTTTTCTAGAAAATCTTCAGATGATTTTCATTCTCTTGTTACCTTCCAGACCAATGCAGTTGGAAGTAGTGGAGGACACAAGAAAAGTCTTGTAGTGGAAGCAAAACAACTGAAGGTAGCCATAAATGGATTTGGAAGAATTGGAAGGAACTTCTTGAGATGTTGGCATGGTCGCAAGGACTCGCCTCTTGATGTCATTGCCATCAATGACACCGGAGGTGTAAAGCAAGCTTCTCACCTTCTCAAGTATGATTCCACACTTGGAATCTTTGATGCTGATGTTAAGCCTGTTGGTACTGATGGCATCTCAGTTGATGGAAAGGTTATCAAAGTTGTCTCGGACCGCAACCCTGCCAACCTTCCTTGGAAGGAGTTGGGGATAGACTTGGTGATTGAAGGAACTGGAGTGTTTGTGGACAGAGAAGGTGCAGGGAGGCACATTACAGCAGGGGCTAAGAAGGTTCTCATCACTGCCCCCGGAAAAGGAGACATCCCTACTTATGTGGTTGGTGTCAATGCTGATGCTTACACCCACGCCGACGACATTATCAGCAATGCTTCTTGCACCACTAACTGCCTTGCTCCCTTTGTCAAGGTCCTTGATCAGAAATTCGGTATTATCAAGGGTACCATGACTACCACTCACTCCTACACCGGTGACCAACGGCTTCTTGACGCGAGCCACCGTGACCTAAGGCGTGCGAGAGCAGCAGCCCTCAACATAGTCCCAACATCAACAGGAGCAGCTAAAGCAGTGGCCCTTGTCCTCCCAACACTCAAAGGCAAGCTCAACGGTATTGCGCTTCGTGTGCCAACACCAAACGTTTCGGTGGTGGACCTCGTCGTTCAAGTCTCAAAGAAGACATTTGCTGAAGAAGTGAATGAGGCTTTTAGAGAGAGTGCAGCCAAGGAGCTGACTGGTATTCTCTCGGTCTGTGACGAGCCACTCGTGTCTGTAGATTTTAGGTGCACCGATGTGTCGTCTACCGTTGATTCGTCATTGACAATGGTCATGGGCGATGACTTGGTTAAGGTGATTGCTTGGTATGATAATGAGTGGGGTTACTCACAAAGGGTTGTTGATTTGGCTGACATTGTTGCCAATAACTGGAAGTAAGAGCCAAATAAGGTTTGTTAATTATATCTATATCTATATATATATGTACGTACCATGTGCCATTGTAATTTTGTACACTTCTTGCTTTATTTTTTTCTTTCTAAACTTGTATTGAGAAACAGAACCATCAATTTTCTCTAGCTCCTCATTGTGCAATAAATTGTTTTACCTTGCTGAAATATACATGTTGATACTAAGCTTCCAAATCTATATATGCACCATTGCACATTGATAATAAAAAACC
TARGET=1396,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2869
PRIMER_LEFT_PENALTY=0.132857
PRIMER_RIGHT_PENALTY=0.154023
PRIMER_LEFT_SEQUENCE=GACATTGTTGCCAATAACTGGA
PRIMER_RIGHT_SEQUENCE=TGCACAATGAGGAGCTAGAGAA
PRIMER_LEFT=1334,22
PRIMER_RIGHT=1515,22
PRIMER_LEFT_TM=59.867
PRIMER_RIGHT_TM=60.154
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=0.2869
PRIMER_LEFT_1_PENALTY=0.132857
PRIMER_RIGHT_1_PENALTY=0.154023
PRIMER_LEFT_1_SEQUENCE=GACATTGTTGCCAATAACTGGA
PRIMER_RIGHT_1_SEQUENCE=TTGCACAATGAGGAGCTAGAGA
PRIMER_LEFT_1=1334,22
PRIMER_RIGHT_1=1516,22
PRIMER_LEFT_1_TM=59.867
PRIMER_RIGHT_1_TM=60.154
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.5346
PRIMER_LEFT_2_PENALTY=0.380537
PRIMER_RIGHT_2_PENALTY=0.154023
PRIMER_LEFT_2_SEQUENCE=GCGATGACTTGGTTAAGGTGAT
PRIMER_RIGHT_2_SEQUENCE=TGCACAATGAGGAGCTAGAGAA
PRIMER_LEFT_2=1260,22
PRIMER_RIGHT_2=1515,22
PRIMER_LEFT_2_TM=60.381
PRIMER_RIGHT_2_TM=60.154
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.5346
PRIMER_LEFT_3_PENALTY=0.380537
PRIMER_RIGHT_3_PENALTY=0.154023
PRIMER_LEFT_3_SEQUENCE=GCGATGACTTGGTTAAGGTGAT
PRIMER_RIGHT_3_SEQUENCE=TTGCACAATGAGGAGCTAGAGA
PRIMER_LEFT_3=1260,22
PRIMER_RIGHT_3=1516,22
PRIMER_LEFT_3_TM=60.381
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.5613
PRIMER_LEFT_4_PENALTY=0.407297
PRIMER_RIGHT_4_PENALTY=0.154023
PRIMER_LEFT_4_SEQUENCE=GTTGTTGATTTGGCTGACATTG
PRIMER_RIGHT_4_SEQUENCE=TTGCACAATGAGGAGCTAGAGA
PRIMER_LEFT_4=1319,22
PRIMER_RIGHT_4=1516,22
PRIMER_LEFT_4_TM=60.407
PRIMER_RIGHT_4_TM=60.154
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=198
=
PRIMER_SEQUENCE_ID=SSR_03035 Ps_contig_mira-and-tgicl-ass_franssen_28297
SEQUENCE=ATGACCTCATATAAATGATCTAGTGACCATGGAACCTTAGGCTAAAATGGTAATCGCAGGTTTAAGTATCATACATACATACAGATAGACTNATCCTTACATGANATCTACCACTTCTTTGTCTTACAAATTTCATCCACTCAATCATAATATCTTCTGTTCAACCAGAAAAAAAAACGACACTACTCTATAGATCACTCAATCACTGCATATACATATCTTCATTGAATTAATCAACATTACTCTCTCTTCCTCCATCTTCTAGATTCTTCCATCATCAACAACAACAACAAACATGGCTGCTGCAGTAACAGCCGCAGTCTCCTTGCCATACTCCAACTCCACTTCCCTTCCGATCAGAACATCTATTGTTGCACCAGAGAGACTTGTCTTCAAAAAGGTTTCATTGAACAATGTTTCTATAAGTGGAAGGGTAGGCACCATCAGAGCTCAGGTTACTACTGAGGCACCTGCTAAGGTAGTAAAGCATTCAAAGAAACAAGATGAAAATATTGTTGTGAACAAGTTCAAACCTAAGGAACCATACGTTGGGAGGTGTCTTCTCAACACAAAGATCACTGGTGATGATGCACCTGGTGAAACTTGGCACATGGTTTTCAGCACTGAGGGAGAGGTTCCTTACAGAGAAGGACAATCTATTGGGATAGTTCCTGACGGTATTGACAAGAATGGCAAGCCTCACAAGCTGAGATTGTATTCAATTGCTAGCAGTGCCATTGGTGATTTTGGAGACTCCAAAACTGTTTCCCTATGTGTGAAACGTCTTGTTTACACAAACGATGCCGGAGAAGTTGTTAAGGGAGTTTGCTCAAATTTCTTGTGCGATTTGAAGCCGGGATCAGAAGTAAAGATTACTGGACCAGTTGGTAAAGAAATGCTTATGCCAAAAGATCCTAATGCTACTGTCATCATGTTGGGAACTGGAACTGGAATCGCCCCATTTCGCTCATTTTTATGGAAAATGTTCTTTGAGAAGCATGAAGATTACCAGTTCAATGGTTTGGCATGGCTCTTCCTAGGTGTCCCTACAAGCAGCTCACTGCTTTATAAGGAGGAATTTGAAAAGATGAAGGAGAAAGCACCCGAGAACTTCAGGCTTGACTTTGCTGTAAGCAGAGAGCAAGTAAACGATAAAGGAGAGAAAATGTACATCCAAACAAGAATGGCTCAATACGCCGAAGAGTTATGGGAATTACTGAAGAAAGACAACACTTTTGTTTACATGTGTGGACTGAAAGGAATGGAAAAGGGAATTGATGACATAATGGTGTCACTCGCTGCCAAAGACGGCATCGATTGGATCGAGTACAAGAGAACGTTGAAGAAGGCGGAGCAATGGAACGTTGAAGTCTATTAATTTCTTTTCACTTGCATTGATAACAAGTTCCAGTTTGTGCAGCAGCTTATCTATATCACCCTCCTGTATTCTGTAGATAGATTACATTACAACCTTGTAATTTTGGTATGACAAAAAATTATACCTTGCTTGTGAACAATATTGTTTACGGAATCGTTGCAAGAAATAATTTTGAGGATCATGCAATATAGTTTATCAATTTCCTGAAATTTAATTGTGAAT
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8114
PRIMER_LEFT_PENALTY=0.454819
PRIMER_RIGHT_PENALTY=0.356592
PRIMER_LEFT_SEQUENCE=GCTAAAATGGTAATCGCAGGTT
PRIMER_RIGHT_SEQUENCE=GTTGGAGTATGGCAAGGAGACT
PRIMER_LEFT=40,22
PRIMER_RIGHT=339,22
PRIMER_LEFT_TM=59.545
PRIMER_RIGHT_TM=59.643
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=1.2496
PRIMER_LEFT_1_PENALTY=0.372557
PRIMER_RIGHT_1_PENALTY=0.877067
PRIMER_LEFT_1_SEQUENCE=TAGTGACCATGGAACCTTAGGC
PRIMER_RIGHT_1_SEQUENCE=CAGCCATGTTTGTTGTTGTTGT
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=301,22
PRIMER_LEFT_1_TM=60.373
PRIMER_RIGHT_1_TM=60.877
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=1.3319
PRIMER_LEFT_2_PENALTY=0.454819
PRIMER_RIGHT_2_PENALTY=0.877067
PRIMER_LEFT_2_SEQUENCE=GCTAAAATGGTAATCGCAGGTT
PRIMER_RIGHT_2_SEQUENCE=CAGCCATGTTTGTTGTTGTTGT
PRIMER_LEFT_2=40,22
PRIMER_RIGHT_2=301,22
PRIMER_LEFT_2_TM=59.545
PRIMER_RIGHT_2_TM=60.877
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=262
PRIMER_PAIR_PENALTY_3=1.4305
PRIMER_LEFT_3_PENALTY=0.372557
PRIMER_RIGHT_3_PENALTY=1.057901
PRIMER_LEFT_3_SEQUENCE=TAGTGACCATGGAACCTTAGGC
PRIMER_RIGHT_3_SEQUENCE=CAGCCATGTTTGTTGTTGTTG
PRIMER_LEFT_3=20,22
PRIMER_RIGHT_3=301,21
PRIMER_LEFT_3_TM=60.373
PRIMER_RIGHT_3_TM=60.058
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=1.5127
PRIMER_LEFT_4_PENALTY=0.454819
PRIMER_RIGHT_4_PENALTY=1.057901
PRIMER_LEFT_4_SEQUENCE=GCTAAAATGGTAATCGCAGGTT
PRIMER_RIGHT_4_SEQUENCE=CAGCCATGTTTGTTGTTGTTG
PRIMER_LEFT_4=40,22
PRIMER_RIGHT_4=301,21
PRIMER_LEFT_4_TM=59.545
PRIMER_RIGHT_4_TM=60.058
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_03036 Ps_contig_mira-and-tgicl-ass_franssen_28297
SEQUENCE=ATGACCTCATATAAATGATCTAGTGACCATGGAACCTTAGGCTAAAATGGTAATCGCAGGTTTAAGTATCATACATACATACAGATAGACTNATCCTTACATGANATCTACCACTTCTTTGTCTTACAAATTTCATCCACTCAATCATAATATCTTCTGTTCAACCAGAAAAAAAAACGACACTACTCTATAGATCACTCAATCACTGCATATACATATCTTCATTGAATTAATCAACATTACTCTCTCTTCCTCCATCTTCTAGATTCTTCCATCATCAACAACAACAACAAACATGGCTGCTGCAGTAACAGCCGCAGTCTCCTTGCCATACTCCAACTCCACTTCCCTTCCGATCAGAACATCTATTGTTGCACCAGAGAGACTTGTCTTCAAAAAGGTTTCATTGAACAATGTTTCTATAAGTGGAAGGGTAGGCACCATCAGAGCTCAGGTTACTACTGAGGCACCTGCTAAGGTAGTAAAGCATTCAAAGAAACAAGATGAAAATATTGTTGTGAACAAGTTCAAACCTAAGGAACCATACGTTGGGAGGTGTCTTCTCAACACAAAGATCACTGGTGATGATGCACCTGGTGAAACTTGGCACATGGTTTTCAGCACTGAGGGAGAGGTTCCTTACAGAGAAGGACAATCTATTGGGATAGTTCCTGACGGTATTGACAAGAATGGCAAGCCTCACAAGCTGAGATTGTATTCAATTGCTAGCAGTGCCATTGGTGATTTTGGAGACTCCAAAACTGTTTCCCTATGTGTGAAACGTCTTGTTTACACAAACGATGCCGGAGAAGTTGTTAAGGGAGTTTGCTCAAATTTCTTGTGCGATTTGAAGCCGGGATCAGAAGTAAAGATTACTGGACCAGTTGGTAAAGAAATGCTTATGCCAAAAGATCCTAATGCTACTGTCATCATGTTGGGAACTGGAACTGGAATCGCCCCATTTCGCTCATTTTTATGGAAAATGTTCTTTGAGAAGCATGAAGATTACCAGTTCAATGGTTTGGCATGGCTCTTCCTAGGTGTCCCTACAAGCAGCTCACTGCTTTATAAGGAGGAATTTGAAAAGATGAAGGAGAAAGCACCCGAGAACTTCAGGCTTGACTTTGCTGTAAGCAGAGAGCAAGTAAACGATAAAGGAGAGAAAATGTACATCCAAACAAGAATGGCTCAATACGCCGAAGAGTTATGGGAATTACTGAAGAAAGACAACACTTTTGTTTACATGTGTGGACTGAAAGGAATGGAAAAGGGAATTGATGACATAATGGTGTCACTCGCTGCCAAAGACGGCATCGATTGGATCGAGTACAAGAGAACGTTGAAGAAGGCGGAGCAATGGAACGTTGAAGTCTATTAATTTCTTTTCACTTGCATTGATAACAAGTTCCAGTTTGTGCAGCAGCTTATCTATATCACCCTCCTGTATTCTGTAGATAGATTACATTACAACCTTGTAATTTTGGTATGACAAAAAATTATACCTTGCTTGTGAACAATATTGTTTACGGAATCGTTGCAAGAAATAATTTTGAGGATCATGCAATATAGTTTATCAATTTCCTGAAATTTAATTGTGAAT
TARGET=279,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8114
PRIMER_LEFT_PENALTY=0.454819
PRIMER_RIGHT_PENALTY=0.356592
PRIMER_LEFT_SEQUENCE=GCTAAAATGGTAATCGCAGGTT
PRIMER_RIGHT_SEQUENCE=GTTGGAGTATGGCAAGGAGACT
PRIMER_LEFT=40,22
PRIMER_RIGHT=339,22
PRIMER_LEFT_TM=59.545
PRIMER_RIGHT_TM=59.643
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=1.7164
PRIMER_LEFT_1_PENALTY=0.454819
PRIMER_RIGHT_1_PENALTY=1.261614
PRIMER_LEFT_1_SEQUENCE=GCTAAAATGGTAATCGCAGGTT
PRIMER_RIGHT_1_SEQUENCE=TGGAGTATGGCAAGGAGACTG
PRIMER_LEFT_1=40,22
PRIMER_RIGHT_1=337,21
PRIMER_LEFT_1_TM=59.545
PRIMER_RIGHT_1_TM=60.262
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=1.9987
PRIMER_LEFT_2_PENALTY=0.454819
PRIMER_RIGHT_2_PENALTY=1.543888
PRIMER_LEFT_2_SEQUENCE=GCTAAAATGGTAATCGCAGGTT
PRIMER_RIGHT_2_SEQUENCE=TTGGAGTATGGCAAGGAGACTG
PRIMER_LEFT_2=40,22
PRIMER_RIGHT_2=338,22
PRIMER_LEFT_2_TM=59.545
PRIMER_RIGHT_2_TM=61.544
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=2.1782
PRIMER_LEFT_3_PENALTY=2.142476
PRIMER_RIGHT_3_PENALTY=0.035772
PRIMER_LEFT_3_SEQUENCE=CCTCCATCTTCTAGATTCTTCCA
PRIMER_RIGHT_3_SEQUENCE=ACCTTAGCAGGTGCCTCAGTAG
PRIMER_LEFT_3=251,23
PRIMER_RIGHT_3=479,22
PRIMER_LEFT_3_TM=58.858
PRIMER_RIGHT_3_TM=59.964
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=2.1782
PRIMER_LEFT_4_PENALTY=2.142476
PRIMER_RIGHT_4_PENALTY=0.035772
PRIMER_LEFT_4_SEQUENCE=CCTCCATCTTCTAGATTCTTCCA
PRIMER_RIGHT_4_SEQUENCE=CCTTAGCAGGTGCCTCAGTAGT
PRIMER_LEFT_4=251,23
PRIMER_RIGHT_4=478,22
PRIMER_LEFT_4_TM=58.858
PRIMER_RIGHT_4_TM=59.964
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=5.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_03037 Ps_contig_mira-and-tgicl-ass_franssen_28305
SEQUENCE=GAAATTAACCCTATAAATAAACGCTCACCTCACAAGTNTCGGGGACCCCAACGCAGCGCAGCCCTGTGATTTCGATCCTTTTCCTCTTTCTTTTCTATCTCTTTTCTCAGAGATCGTTCTTTTCTAGGGTTTCCGTCTCGTTTTCTCCGCGCTTTCCCTTCCCAGTTCCCACTCGTATCTATTTTCTTCGAATTGATTTTGGTGGTGGGTTGAAGGTATTGAAAGCTGCGGCGTTTTAACCAGACCTTATCAAAACAGCAGGACTGAATGAGCTAATGGAGTCTAAAGGAGGTAAAAAGAAGTCTAGTAGTAGTAGTAATTCCTCATTGTTCTACGAAGCCCCCCTCGGATACAGCATTGAAGACATTCGTCCAAACGGTGGAATCAAGAAGTTCAGATCAGCTGCTTACTCCAACTGCGCTCGCAAGCCATCCTGATATACCGTCCTGATTCTCGTTCCCGTGCATGCAAGCACTTTTCTATTAGCTTTTTTTGTTTTCCTTTAGCTTTTTTCACTGCTCTCCTTCAAACAACATCTTTCCAAATCCTCTCCTTGCCACTATAATTTTTGTTTCTCTCCTTGTTTCTTTTGTATTTCTTTCTGTTTGAACTGTGGCCATGGCAGTTTCTGCAATTGGTTTTGAAGGTTTCGAAAAGAGGTTGGAAATATCCTTTTCTGACCCAGGACTTTTCTCTGACCCTCAAGGAAGGGGATTACGATCTCTCACAAAATCCCAATTGGACGAGATTCTTGCACCAGCTGAGTGCACCATTGTTTCTTCCCTTGCAAATGAGGATGTTGATTCCTATGTTCTATCTGAGTCCAGCCTTTTTGTTTATGCCTACAAGCTCATAATCAAAACCTGCGGCACTACCAAGTTACTGCTTTCAATCCCACCAATATTGAAATTGGCCGACTCCATTTCTCTTAATGTTAGATCTGTGAGGTACACCCGAGGCAGCTTCATTTTCCCTGGTGCTCAGTCTTTTCCTCATCGCCACTTTTCCGAAGAAGTAGCTGTCCTTGATGGCTTCTTTGGCAAACTTGGATCAGGTAGCATGGCTTATATTCTGGGTGGATCCGATGAAGCCCAGAATTGGCATATCTACTGTGCCTCTTCTGATTCCGTGAGCCCAGAAGGTTCTGTCTACACACTTGAGATGTGTATGACCGGCTTAGACCGAGAGAAAGCCTCCGTTTTCTTTAAAGAACAAACAGGTTCGGCTGCCGAGATGACCGTCAACTCTGGCATTAGGAAAATTCTTCCAAATTCTGAAATTTGTGACTTTGATTTTGAACCTTGTGGTTATTCAATGAATTCTGTTGAGGGGCCTGCTGTATCTACCATTCATATTACTCCAGAAGACGGTTTCAGTTATGCAAGTTTTGAGACAGCAGGATACGATTTGAAGGCAATAAATCTGAACGAAATGGTGATGAGGGTGCTGGCTTGTTTCCAACCAACTGAGTTTTCTGTAGCTGTTCACGTGGACAATGCAAGCAAGTCATTTGAGCAGGGGTGTTTGCTGGATGTTAAGGGATACTGCTGTGAAGAGAAGAGCCACCAAGGGCTTGGAATGAGTGGATCTGTTGTTTACCAAAAATTTCTCAAGACTTCCTACTGTGGCTCGCCTAGATCCACACTCAAGTGCTGGAAAGATGAAGATGAAGAAGAGTAGCATTTGTGTCTTTCTGTGTGTCTGTTTAAAATAAGTGTTGCCTTGATGGCTTAAACACATCGTTTCGTTTATTTGCTGTTTATGTGTCTAGAAGGTTATAACCTTCATTGTTGTTTTCTTTTATTTGCTGTTTCTGTCTAGAAGGTATGCCTTCATTGTTATTTTCGTTTAAACTATGAAATTGAAATAAAGTGCATGTTATGGGAATTNCTTGGACTAAAATTTT
TARGET=305,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1166
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=CAAAACAGCAGGACTGAATGAG
PRIMER_RIGHT_SEQUENCE=TGTTTGAAGGAGAGCAGTGAAA
PRIMER_LEFT=250,22
PRIMER_RIGHT=531,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.1166
PRIMER_LEFT_1_PENALTY=0.086339
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=CAAAACAGCAGGACTGAATGAG
PRIMER_RIGHT_1_SEQUENCE=TTGTTTGAAGGAGAGCAGTGAA
PRIMER_LEFT_1=250,22
PRIMER_RIGHT_1=532,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.1548
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.154023
PRIMER_LEFT_2_SEQUENCE=GGGTTGAAGGTATTGAAAGCTG
PRIMER_RIGHT_2_SEQUENCE=TGGAGTAAGCAGCTGATCTGAA
PRIMER_LEFT_2=206,22
PRIMER_RIGHT_2=413,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.154
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.1548
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.154023
PRIMER_LEFT_3_SEQUENCE=GGGTTGAAGGTATTGAAAGCTG
PRIMER_RIGHT_3_SEQUENCE=TTGGAGTAAGCAGCTGATCTGA
PRIMER_LEFT_3=206,22
PRIMER_RIGHT_3=414,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.2374
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.236656
PRIMER_LEFT_4_SEQUENCE=GGGTTGAAGGTATTGAAAGCTG
PRIMER_RIGHT_4_SEQUENCE=GCAGCTGATCTGAACTTCTTGA
PRIMER_LEFT_4=206,22
PRIMER_RIGHT_4=405,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.763
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_03038 Ps_contig_mira-and-tgicl-ass_franssen_28309
SEQUENCE=CCGACGATACTCATCCTCTAAAGACTTGGGGGAACGGTCCCAATGAAAAAGTGTCACATATGGTTGCAAACTTATCCTAAATTCTTAAAACTCTAAACATATTGCAATGCTACATGAATTCAAAAACAAAACTTCCTTATCAACTAATGCATAAATTAATATCTTCTAAACAAGGGCGGAAAAAAATTCATAGGGCATGTAATGCATAGATAATATGCCATGATTTTCATTCCTTAAAGTCATCTTTGTCATCAATGGGAATTGGAGTATTCCTCCAATACACAGCTAGAGCACTCCCACCAAGCGCCATGCAGACAACGCTGACAGCAGAAGGAACAGCCACAAGGGGGTTTGTGAAGTGTTTCTGAGCAAGCAAGAATCCAAGTGCAGAACTCTGCATCCCACACTCAATCGATATTGTACGTGATGTGGATTCGCCAAATGACAGTTTCGAAATCCAATAACCAATAGCAAATGCAGCAGCATGTAGAGCAAGAACTGGCAATATCAATTGTGCTCCTTGAGTTTTTAAGACCTCTGAAACTTGCCCTATTGGGCTTGCACAAAGGAGAGTGGTCAAGATAACTCCTATCAAAGGTGTGACTGTGATTATCTTTGAAGTGAACTTGGGGAAAAACTCATTTGCCAAAACTCCCACAATTGTAGGGACTAAAACAACCTGAAAGGTGCTTAATGCCAGGCCAGCAGCATCAACTGGAACAAGTTGACCAGCTAGAAGCTTTGTTAGAAGTGGGGTCATAATAATAGCTCCGACAGTTGAACACGTCGTCATTAGAACAGAGAGAGCAACGTTTCCCTTGGATATATATGTTGCAACATTTGATGCCTGGCCTCCAGGACAACATGAGACCAAAATAAGACCCGTTGCAAGAGGAGGTGAAAGTTTTAGTGACATTGCAATGACAAAGCCTAGCAAGGGCTTGACAAAATATTGTGCAAGAAACCCACACCCACAGTCCACGGATTTCGCAGACATCGTTTGAAATCGTCAAATGTCAATGTCAAACCCATAGAGAGCATGAGGAAACCCAAGCCAAGAGTAAAAAGGTCCGTCTCCAACCAAGTAACAGCAGCTGGCTTGTAAATACCGAGGATAGCCCCCAAGATGACCCACAGGGGAAAAAGAGTGGTTAGAGTTTCAATAACTTTCTCATACTGGGTCAGGCCCCCGGCACTTTCAGGAATATCTCCAGAAATGTTTGTTGTAGCTGAGCACAATATCTGTGAGTTCCTTTTAGAACTCAGAGAAACTAAAGGGAATCTAGCAGCCGCAACAATCGTTTTTTCGCCCCACAATTCACTTCTAACCGCACAGCTTCTTCCAAAATCGCTTCTCACGTCTAGATGGCTATTAACAAAGTTTCTCCGAGGGAGATAAGTATAATTTGGCTTAAGAATCGCATCGAACGATCCAATTCTATGGTCTCTGAGAATGATTCTGGGCATGGAAGCCATTGATTAGAGAGAGAGAAAGGGTTTAATGTGAAAAGGGGTTTATTGATTCCGTTAGATATTAATATAGTAGAAAAGGAAATGGAGAAAGCGAAGTAGTGGGAAATTGGAGTGTGGAAATGAAGTAGATTCAAAATTGAAATCCCCTAC
TARGET=1482,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1171
PRIMER_LEFT_PENALTY=0.102139
PRIMER_RIGHT_PENALTY=0.014945
PRIMER_LEFT_SEQUENCE=TTAACAAAGTTTCTCCGAGGGA
PRIMER_RIGHT_SEQUENCE=ACCCCTTTTCACATTAAACCCT
PRIMER_LEFT=1372,22
PRIMER_RIGHT=1514,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=59.985
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=143
PRIMER_PAIR_PENALTY_1=0.2083
PRIMER_LEFT_1_PENALTY=0.193337
PRIMER_RIGHT_1_PENALTY=0.014945
PRIMER_LEFT_1_SEQUENCE=CAATTCACTTCTAACCGCACAG
PRIMER_RIGHT_1_SEQUENCE=ACCCCTTTTCACATTAAACCCT
PRIMER_LEFT_1=1314,22
PRIMER_RIGHT_1=1514,22
PRIMER_LEFT_1_TM=59.807
PRIMER_RIGHT_1_TM=59.985
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.2099
PRIMER_LEFT_2_PENALTY=0.102139
PRIMER_RIGHT_2_PENALTY=0.107763
PRIMER_LEFT_2_SEQUENCE=TTAACAAAGTTTCTCCGAGGGA
PRIMER_RIGHT_2_SEQUENCE=TTTCCCACTACTTCGCTTTCTC
PRIMER_LEFT_2=1372,22
PRIMER_RIGHT_2=1578,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.3011
PRIMER_LEFT_3_PENALTY=0.193337
PRIMER_RIGHT_3_PENALTY=0.107763
PRIMER_LEFT_3_SEQUENCE=CAATTCACTTCTAACCGCACAG
PRIMER_RIGHT_3_SEQUENCE=TTTCCCACTACTTCGCTTTCTC
PRIMER_LEFT_3=1314,22
PRIMER_RIGHT_3=1578,22
PRIMER_LEFT_3_TM=59.807
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=265
PRIMER_PAIR_PENALTY_4=0.3187
PRIMER_LEFT_4_PENALTY=0.102139
PRIMER_RIGHT_4_PENALTY=0.216588
PRIMER_LEFT_4_SEQUENCE=TTAACAAAGTTTCTCCGAGGGA
PRIMER_RIGHT_4_SEQUENCE=CCACTACTTCGCTTTCTCCATT
PRIMER_LEFT_4=1372,22
PRIMER_RIGHT_4=1574,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=59.783
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_03039 Ps_contig_mira-and-tgicl-ass_franssen_28310
SEQUENCE=AAGTTAACTTAAACTTTGAATTTTATTCATTGGCAATTCTTTTTATTTCACAAAGGACTACATGTGAGTGAGTTTCATTTCACTTTTCTGTAATCCTGACTTTAACAACAACAACAACAACAAAAACATATCCCATTAAATGGGTCGGAAAATCCCAAGTTCATCAAAACAAAATCATTTTCTTGAAACCACATGTAATATATATGCCTAAGATTTAACGAGAAAATTCTTGAACCATTCAGCAGAAAGTTTTGGGTGTCTTTTCTGACCGTCTTTAAAATCAACGAAGACGAGTCCAAATCTCAAGCTCAAGCCAGAATCCCATTCAAAGTTATCCAACAATGACCATGCAAAATATCCCTTTACATTCACACCATCCCTAATTGCAGTTTCAAGATAGTAAAGATGACGATAGTAGTAATCAATCCTATAAGTATCTAACAGAGATTCTTCAAGAGATAATGTTGGATCGTTGAACTCATCACGACCATTTTCAGTAATGTAAATTACAGGGTCCTCGTACGTTTTCTTGGTGTAAAGCAAAAGATTATGAAGTCCTTTTGGATAAATACATAACCAACTCGAAGCAGACATTGGACCGAGAGGCTTGCCTTTATGTTCAAATGTAGCATTAATACGAGAATCAGTTTGTATGGCCGGTTGCACGGCATTGGGAGGGTGAGGTGCATGAGCAGCATAGTAGGATGAGTAATAGTTTAGGCCTAGAAAATCAAAAGACCCCTTAAGTTCCCTTGACTCTTCTTTTGAGAACTTTGGTAATCTCTTTCCCACCAAAATTCGCATGCTTTTTGGATATTCGCCTCTCGTAAGCGGATTCATATACCATCCAAACATGAAGTCGAGACCTCGTTTTGAAGCATCAACGTCGGCCTTCGCTGTAGTGGCCGGCTCATACCAGTGTGAGACTAAGGTAATCCCTATTATGCCTCGTTGAGATGACTTATATTTGATCCGGTACAAATTTGCAGCAGCAGAATGAGCAAGAAGCTGGTTGTGTGCAGCCAAATATGGTTCTGTCCCTGAATCACCTCCTGTGCAATTCAGATTTAACCAATCTGAACATCGACCCGGTGCGAACTTTCCGAATGCATAAGCATTCATGCTCAAAGTCGATGGTTCATTTATAGTAATCCAATGTTTCACTCTATCACCAAATTCCTTGAAGCAAAGTTCAGCATAGTCTCTAAAATCATCTGCAATGCGACGACTTAACAAACCGCGATACTCATCCTCTAAAGCTTGGGGAACGTCCCAATGAAAAAGTGTCACATATGGTTGCAAACCCTTAGCCAATAATTCGTTGATGAGATTGTTGTAGTACTTTATTCCTTCAGGATTTACACCTCCGCTAAGCTTTCCCTTTGGTAGAACTCTAGACCAAGAAATGGAGAATCTATAAGCATCCATATTCAAATCCTTTATGAGTTCAATATCTTCTTTGTACTTATGATAGGAGTCAACCGCTACATCACCATTACTTCTATCTCTTTATTTTTTCTGGATATTTATGAGTGAAGTTATCCCAAATGCTTGGTCCTTTCCACCCTCAAATGCTGCACCTTCATACTGATATGCAGAAGGGCTGTTACCAAAAAACAAAGCCTTTAGGGAAAACTGCTTCCGGTTAGAAAATCCAACCGAAGGTAAGGGTTTAAAACCAATTTCTAACGTAAGGGGTAAACGTAA
TARGET=104,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.1633
PRIMER_LEFT_PENALTY=3.158939
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=CACAAAGGACTACATGTGAGTGAG
PRIMER_RIGHT_SEQUENCE=ATTTGGACTCGTCTTCGTTGAT
PRIMER_LEFT=48,24
PRIMER_RIGHT=300,22
PRIMER_LEFT_TM=58.841
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=45.833
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=3.1845
PRIMER_LEFT_1_PENALTY=3.158939
PRIMER_RIGHT_1_PENALTY=0.025566
PRIMER_LEFT_1_SEQUENCE=CACAAAGGACTACATGTGAGTGAG
PRIMER_RIGHT_1_SEQUENCE=GCTTGAGCTTGAGATTTGGACT
PRIMER_LEFT_1=48,24
PRIMER_RIGHT_1=313,22
PRIMER_LEFT_1_TM=58.841
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=45.833
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=3.2671
PRIMER_LEFT_2_PENALTY=3.158939
PRIMER_RIGHT_2_PENALTY=0.108206
PRIMER_LEFT_2_SEQUENCE=CACAAAGGACTACATGTGAGTGAG
PRIMER_RIGHT_2_SEQUENCE=GCTTGAGATTTGGACTCGTCTT
PRIMER_LEFT_2=48,24
PRIMER_RIGHT_2=307,22
PRIMER_LEFT_2_TM=58.841
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=45.833
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=3.2734
PRIMER_LEFT_3_PENALTY=3.158939
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=CACAAAGGACTACATGTGAGTGAG
PRIMER_RIGHT_3_SEQUENCE=GGTCAGAAAAGACACCCAAAAC
PRIMER_LEFT_3=48,24
PRIMER_RIGHT_3=269,22
PRIMER_LEFT_3_TM=58.841
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=45.833
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=3.4020
PRIMER_LEFT_4_PENALTY=3.158939
PRIMER_RIGHT_4_PENALTY=0.243031
PRIMER_LEFT_4_SEQUENCE=CACAAAGGACTACATGTGAGTGAG
PRIMER_RIGHT_4_SEQUENCE=GAGATTTGGACTCGTCTTCGTT
PRIMER_LEFT_4=48,24
PRIMER_RIGHT_4=303,22
PRIMER_LEFT_4_TM=58.841
PRIMER_RIGHT_4_TM=59.757
PRIMER_LEFT_4_GC_PERCENT=45.833
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_03040 Ps_contig_mira-and-tgicl-ass_franssen_28311
SEQUENCE=GGAAAAGACTTAGCGGAGGTGTAAATCCTGAAGGAATAAAGTACTACTACAACAATCTCATCAACGGATTATTGGCTAAGGGTTTGCAACCATATGTGACACTTTTTCATTGGGACGTTCCCCAAGCTTTAGAGGATGAGTATCACGGTCTTTTAAGCCATCGCATTGTAGATGATTTTAGAGACTATGCTGAACTTTGCTTCAAGGAATTTGGTGATAGAGTGAAACATTGGATTACTATAAATGAACCATGGACTGTGAGCATGAATGCTTATGCATTCGGAACGTTGGCACCGGGTCGATGTTCATATTGGTTAAATCGGAATTGCACAGGAGGTGATTCAGGGACAGAACCATATTTGGCTGCACACAACCAGCTTCTTGCTCATTCTGCTGCTGCAAATTTGTACCGGATCAAATATAAGTCATCTCAACGAGGCATAATAGGGATTACCTTAGTCTCACACTGGTATGAGCCGGCCACTACAGCGACGGCCGACGTTGATGCTTCAAAACGAGCTCTCGACTTCATGTTTGGATGGTATATGAATCCGCTTACAAGAGGCGAATATCCAAAAAGCATGCGAACTTTGGTGGGAAAGAGATTACCAAAGTTCTCAAAAGAAGAGTCAAGGGAACTTAAGGGGTCTTTTGATTTTCTAGGCCTAAACTATTACTCATCCTACTATGCTGCTCATGCATCTCACCATCCCAATGTCGTCCAACCGGCCATACAAACTGATTCTCTTGTTAATGCTACATTTGAACATAATGGCAAGCCTCTCGGTCCAATGTCTGCTTCGAGTTGGTTATGTATTTATCCAAAAGGACTTCATAATCTTTTGCTTTACACCAAGAAAACGTACGAGGACCCTGTAATTTACATTACTGAAAATGGTCGTGATGAGTTCAACGATCCAACATTATCTCTTGAAGAATCTCTCTTAGATACTTATAGGATTGATTACTACTATCGTCATCTTTACTATATTGAAACTGCAATTAGGCATGGTGTGAATGTAAAGGGATATTTTGCATGGTCATTGTTGGATAACTTTGAATGGGATTCTGGCTCGAGCTTGAGATTTGGACTCGTCTTCGTTGATTTTAAAGACGGTCAGAAAAGACACCCAAAACTTTCTGCTGAATGGTTCAAGAATTTTCTCGTTAAATCTTAGGCATATATATTACATGTGGTTTCAAGAAAATGATTTTGTTTTGATGAACTTGGGATTTTCTGACCCATTTAATGGGATATTGTTTTTTTTTGTTGTTGTTGTTAAAGTCAGGATTACAGAAAAGTGAAATGAAACTCACTCACATGTAGTCCTTGGTGAAATAAAAATAATTGTCAATGAATGAATTCAAAGTTTGTAAAATAACTTCTTACTCGCACTCTTTTAATACAATGGAATTATAYANANTGTGAC
TARGET=1268,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03041 Ps_contig_mira-and-tgicl-ass_franssen_28313
SEQUENCE=GACACATGTGAGTGAGTTTCTATTCACTTTTCTTGTAATCCTGACTTTAACTAACAACAACAACAAAAAACAATATCCCATTAAATGGGTCGGAAATACCCAAGTTCATCAAAACAAAANCATTTCTTGAAACCACATGTAATATATATGCCTAAGTTTATTTTTTAACGAGAAAATTCTTGAACCATTCAGCAGAAAGTTTTGGGTGTCTTTTCTGACCGTCTTTAAAATCAACGAAGACGAGTCCAAATCTCAAGCTCAAGCCAGAATCCCATTCAAAGTTATCCAACAATGACCATGCAAAATATCCCTTTACATTCACACCATCCCTAATTGCAGTTTCAAGATAGTAAAGATGACGATAGTAGTAATCATCCTATAGTACTCTGCGTT
TARGET=53,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03042 Ps_contig_mira-and-tgicl-ass_franssen_28324
SEQUENCE=AAGACAGTGGGTATCAACGCAGAGTACGCGGGTACAGAAGAAAGAGCAAAAGCAGAGTCTCTCTTCAAAGCTTATTTCAGAGGCAGAATCATAGAGATTAACACCAATGGGAGAGGAAGAAAAGAAAACAGAGGAAACCAAAACAGAGGAAGAAGAAACCAAGGCAGAGGAAAAACCGGAGGAAACAAAAGAATGAAGAAGCTGCTCCGCCACCGCCTCCGGCAGAGATTTTGCTGAAAGTGTTTATGCATTGTGAAGGCTGTGCTCGGAAAGTTCGTCGCTGTTTGAAGAATTTCCCCGGAGTTGAAGGTGTGACAACTAACTGCAAAACACATGAGGTTGTTGTGAAAGGTGAGAAAGCTGATCCGGCGAAGGTTGTGGAGAGAATTCAGACGAAGAGTCGGAGGAAGGTTGAGCTTGTATCTCCGCTCCCGAATCCACCAGAGGAGGAAAAAGTTGTTGAGGAAGAAGAGAAACCAGAACCTGAAGAGGAGAAGAAAGAAGAGCCTCAGATTGTAACGGTTGTTCTTAAAGTTCACATGCATTGTGAAGGTTGTGCCGAAGAGATAAAAAAACGCATACTCAAAATGAAGGGAGTGGAATCGGTGGAAACTGATTTGAAGAAGTCTGAGGTAACCGTGAAGGGAGTGTATGATCCAACGATGTTGGTTAAATTCGTGTACAAACGATTTGGGAAGCAAGCTGTGATTGTGAATGAAGAGAAAGTCGAGGAAGAGAAAAAAGAAGAGGCTGTGATTGTGAATGAAGAGAAAGTCGAGGAAGAAGCAAAAGCCGAGGAAGAGAAAAAAGAAGAGACGAATGAAGAAGTGGAGGAGAAGAAAGAAGAAGAACAGAATGAGGTGAAACCACAAGAGAATCCAGAAGGTGCAATTGTTGTACAAGAGACCAAGGTGGAAGATGAGATGAAGAAAAATGAATCCTACTATAATCCACCAATGGATTTGTATGCACAACCACCACCAATGGATTATCATGCATACCCACCAATAGCTTATCCTGCATGTCCTCCATACTATCAAACATATCTTCCACCAGCACCGCAGATCTTCAGCGACGAGAATCCAAATGCTTGCAGTGTTATGTGAAATTTAATTTCACAAATATCAAGGGTCATATTAAAAATAAGAGATAGTAATTAGTTTTATTTTGCTAAATATACTAGAGTATACATGAACCATGGAATTTTTTATTTTGTAAGATTTTTATACTTTTGTTGAAGTGAGAATTTCTATTTTGTTTTAAAGGCATGTGAGAAAGTTAGGGAAAAGTTTGTTATGATATACAAACAAACAAGCATCTATCTTAGATGGAAAGCATTATGCTTTTGTTTTAGCCTCTTCTGATAGAGTTTTGGACGCTATGTTTGTGTGTTTTTAGGAAAGG
TARGET=1303,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5915
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.461118
PRIMER_LEFT_SEQUENCE=AGGCATGTGAGAAAGTTAGGGA
PRIMER_RIGHT_SEQUENCE=AACACACAAACATAGCGTCCAA
PRIMER_LEFT=1263,22
PRIMER_RIGHT=1392,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.461
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=0.5915
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.461118
PRIMER_LEFT_1_SEQUENCE=AGGCATGTGAGAAAGTTAGGGA
PRIMER_RIGHT_1_SEQUENCE=ACACACAAACATAGCGTCCAAA
PRIMER_LEFT_1=1263,22
PRIMER_RIGHT_1=1391,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.461
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.5915
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.461118
PRIMER_LEFT_2_SEQUENCE=AGGCATGTGAGAAAGTTAGGGA
PRIMER_RIGHT_2_SEQUENCE=AAACACACAAACATAGCGTCCA
PRIMER_LEFT_2=1263,22
PRIMER_RIGHT_2=1393,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=60.461
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=131
PRIMER_PAIR_PENALTY_3=0.8082
PRIMER_LEFT_3_PENALTY=0.347129
PRIMER_RIGHT_3_PENALTY=0.461118
PRIMER_LEFT_3_SEQUENCE=AAGGCATGTGAGAAAGTTAGGG
PRIMER_RIGHT_3_SEQUENCE=AACACACAAACATAGCGTCCAA
PRIMER_LEFT_3=1262,22
PRIMER_RIGHT_3=1392,22
PRIMER_LEFT_3_TM=59.653
PRIMER_RIGHT_3_TM=60.461
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=0.8082
PRIMER_LEFT_4_PENALTY=0.347129
PRIMER_RIGHT_4_PENALTY=0.461118
PRIMER_LEFT_4_SEQUENCE=AAGGCATGTGAGAAAGTTAGGG
PRIMER_RIGHT_4_SEQUENCE=AAACACACAAACATAGCGTCCA
PRIMER_LEFT_4=1262,22
PRIMER_RIGHT_4=1393,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=60.461
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_03043 Ps_contig_mira-and-tgicl-ass_franssen_28336
SEQUENCE=GATTACGCTCTGCACTAAACCCTCCATCGCCGTTCACATCAATCTCTTCGCAGTCTCTCTAAAAATGGCTCCTCCTCCTCGAGGTCGCGGTGGTTCTGGCGGTGGTTTCAGAGGTAGGGGCGGTGACAGAGGAGGTAGAAGTTTTGGTGGAGGAAGAGGAGGAGATAGAGGTACACCTTTTAAGGCTCGAGGTGGTGGTGGTAGAGGTGGAGGCGGCAGAGGAGGTCGTGGAGGAGGCCGCGGGGCGGGAGAGGCGGGATGAAGGGAGGAAGCAAGGTTGTTGTTGAACCTCATAGACACGACGGAATTTTCATTGCTAAGGGTAAAGAAGATGCTCTTGTTACTAGAAATCTTGTTCCCGGCGAAGCTGTTTACAATGAGAAGAGGGTTTCCGTGCAGAGTGAGGATGGTACCAAGGTTGAGTATAGAATTTGGAACCCTTTCCGATCTAAGCTGGCAGCTGCAGTTCTTGGTGGAGTTGACCATATATGGATTAAACCTGGTGCTAAAGTACTTTACTTAGGAGCTGCATCTGGAACAACAGTCTCTCATGTGTCTGATATTGTTGGTCCAACTGGAGTTGTTTATGCGGTGGAATTCTCCCATCGAAGTGGGCGTGACTTGGTCAATATGGCTAAGAAACGGACTAATGTTATCCCGATTATTGAGGATGCTAGACATCCAGCCAAGTACAGAATGTTGGTTGGCATGGTAGATGTGATATTTTCTGATGTTGCTCAGCCTGATCAGGCAAGGATTTTAGGGTTGAATGCTTCGTACTACCTCAAAGCTGGAGGTCATTTTGTTATTTCTATCAAGGCCAATTGTATCGATTCCACAGTCCCTGCTGAGGCAGTGTTCACCAGTGAAGTGAACAAGTTGAAAGCAGATCAATTTAAACCATTTGAGCAAGTGACTCTTGAACCATTTGAGAGGGACCATGCTTGTGTTGTTGGTGGCTACAGAATGCCTAAGAAGAAGAAAGATGCTGAGTAACCTTATTTTGTTGTTTAAAATGTACTATTGTATCACTTAAGCAACTTGGGATGTCTTTGATTACCTGGATTAATGTGTTGCTTCTGAACTAAAAAAGTATGTGCCATTACTCACAAGTATTTCAAATCGATTTTAATTTTTATGTTGGTAAAGCTCATTTGACCAAAAGG
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1217
PRIMER_LEFT_PENALTY=0.118120
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=TTCACATCAATCTCTTCGCAGT
PRIMER_RIGHT_SEQUENCE=TGAAAATTCCGTCGTGTCTATG
PRIMER_LEFT=32,22
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=59.882
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.1482
PRIMER_LEFT_1_PENALTY=0.118120
PRIMER_RIGHT_1_PENALTY=0.030114
PRIMER_LEFT_1_SEQUENCE=TTCACATCAATCTCTTCGCAGT
PRIMER_RIGHT_1_SEQUENCE=CCTTAGCAATGAAAATTCCGTC
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=321,22
PRIMER_LEFT_1_TM=59.882
PRIMER_RIGHT_1_TM=59.970
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=0.2263
PRIMER_LEFT_2_PENALTY=0.118120
PRIMER_RIGHT_2_PENALTY=0.108206
PRIMER_LEFT_2_SEQUENCE=TTCACATCAATCTCTTCGCAGT
PRIMER_RIGHT_2_SEQUENCE=AATTCCGTCGTGTCTATGAGGT
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=308,22
PRIMER_LEFT_2_TM=59.882
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.2263
PRIMER_LEFT_3_PENALTY=0.118120
PRIMER_RIGHT_3_PENALTY=0.108206
PRIMER_LEFT_3_SEQUENCE=TTCACATCAATCTCTTCGCAGT
PRIMER_RIGHT_3_SEQUENCE=ATTCCGTCGTGTCTATGAGGTT
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=307,22
PRIMER_LEFT_3_TM=59.882
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.3174
PRIMER_LEFT_4_PENALTY=0.118120
PRIMER_RIGHT_4_PENALTY=0.199246
PRIMER_LEFT_4_SEQUENCE=TTCACATCAATCTCTTCGCAGT
PRIMER_RIGHT_4_SEQUENCE=ACTTCTACCTCCTCTGTCACCG
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=141,22
PRIMER_LEFT_4_TM=59.882
PRIMER_RIGHT_4_TM=59.801
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_03044 Ps_contig_mira-and-tgicl-ass_franssen_28336
SEQUENCE=GATTACGCTCTGCACTAAACCCTCCATCGCCGTTCACATCAATCTCTTCGCAGTCTCTCTAAAAATGGCTCCTCCTCCTCGAGGTCGCGGTGGTTCTGGCGGTGGTTTCAGAGGTAGGGGCGGTGACAGAGGAGGTAGAAGTTTTGGTGGAGGAAGAGGAGGAGATAGAGGTACACCTTTTAAGGCTCGAGGTGGTGGTGGTAGAGGTGGAGGCGGCAGAGGAGGTCGTGGAGGAGGCCGCGGGGCGGGAGAGGCGGGATGAAGGGAGGAAGCAAGGTTGTTGTTGAACCTCATAGACACGACGGAATTTTCATTGCTAAGGGTAAAGAAGATGCTCTTGTTACTAGAAATCTTGTTCCCGGCGAAGCTGTTTACAATGAGAAGAGGGTTTCCGTGCAGAGTGAGGATGGTACCAAGGTTGAGTATAGAATTTGGAACCCTTTCCGATCTAAGCTGGCAGCTGCAGTTCTTGGTGGAGTTGACCATATATGGATTAAACCTGGTGCTAAAGTACTTTACTTAGGAGCTGCATCTGGAACAACAGTCTCTCATGTGTCTGATATTGTTGGTCCAACTGGAGTTGTTTATGCGGTGGAATTCTCCCATCGAAGTGGGCGTGACTTGGTCAATATGGCTAAGAAACGGACTAATGTTATCCCGATTATTGAGGATGCTAGACATCCAGCCAAGTACAGAATGTTGGTTGGCATGGTAGATGTGATATTTTCTGATGTTGCTCAGCCTGATCAGGCAAGGATTTTAGGGTTGAATGCTTCGTACTACCTCAAAGCTGGAGGTCATTTTGTTATTTCTATCAAGGCCAATTGTATCGATTCCACAGTCCCTGCTGAGGCAGTGTTCACCAGTGAAGTGAACAAGTTGAAAGCAGATCAATTTAAACCATTTGAGCAAGTGACTCTTGAACCATTTGAGAGGGACCATGCTTGTGTTGTTGGTGGCTACAGAATGCCTAAGAAGAAGAAAGATGCTGAGTAACCTTATTTTGTTGTTTAAAATGTACTATTGTATCACTTAAGCAACTTGGGATGTCTTTGATTACCTGGATTAATGTGTTGCTTCTGAACTAAAAAAGTATGTGCCATTACTCACAAGTATTTCAAATCGATTTTAATTTTTATGTTGGTAAAGCTCATTTGACCAAAAGG
TARGET=191,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0451
PRIMER_LEFT_PENALTY=0.041553
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=CGCAGTCTCTCTAAAAATGGCT
PRIMER_RIGHT_SEQUENCE=TGAAAATTCCGTCGTGTCTATG
PRIMER_LEFT=48,22
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=60.042
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.0717
PRIMER_LEFT_1_PENALTY=0.041553
PRIMER_RIGHT_1_PENALTY=0.030114
PRIMER_LEFT_1_SEQUENCE=CGCAGTCTCTCTAAAAATGGCT
PRIMER_RIGHT_1_SEQUENCE=CCTTAGCAATGAAAATTCCGTC
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=321,22
PRIMER_LEFT_1_TM=60.042
PRIMER_RIGHT_1_TM=59.970
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=274
PRIMER_PAIR_PENALTY_2=0.1217
PRIMER_LEFT_2_PENALTY=0.118120
PRIMER_RIGHT_2_PENALTY=0.003589
PRIMER_LEFT_2_SEQUENCE=TTCACATCAATCTCTTCGCAGT
PRIMER_RIGHT_2_SEQUENCE=TGAAAATTCCGTCGTGTCTATG
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=312,22
PRIMER_LEFT_2_TM=59.882
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=281
PRIMER_PAIR_PENALTY_3=0.1482
PRIMER_LEFT_3_PENALTY=0.118120
PRIMER_RIGHT_3_PENALTY=0.030114
PRIMER_LEFT_3_SEQUENCE=TTCACATCAATCTCTTCGCAGT
PRIMER_RIGHT_3_SEQUENCE=CCTTAGCAATGAAAATTCCGTC
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=321,22
PRIMER_LEFT_3_TM=59.882
PRIMER_RIGHT_3_TM=59.970
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.1498
PRIMER_LEFT_4_PENALTY=0.041553
PRIMER_RIGHT_4_PENALTY=0.108206
PRIMER_LEFT_4_SEQUENCE=CGCAGTCTCTCTAAAAATGGCT
PRIMER_RIGHT_4_SEQUENCE=ATTCCGTCGTGTCTATGAGGTT
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=307,22
PRIMER_LEFT_4_TM=60.042
PRIMER_RIGHT_4_TM=59.892
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=260
=
PRIMER_SEQUENCE_ID=SSR_03045 Ps_contig_mira-and-tgicl-ass_franssen_28338
SEQUENCE=ATCCTTGTTTTGTGGTACATGGTCTACNTNGGGACTGGATTATTATTAATTTTCACAAACTTCACCAAAACTTCGAAGTACCTTCATTATACTCTTTCACTTCACTCCATAATCATCCACACTTAGTCAAACAAAACATAAAAATGGCAACCATCTCAGCTACCACCACAACCGCATCAATCTCCCGTGCATGTCTTGCACAGAAGCGACCACTTACAATCTCATCCTCTCCTGTTCTTGGATTACCAACAATGGCTAAGGTTGGAAGAGTGAGGTGTTCAGTGGAAGGAAAGCCTTCATCTGTGAACGAAAGCAGCTCAAAGATGGGAATGACAGCGTCTTTGTTATCAGCTGCATGTGCTGCAGCCATGTCAAGCCCAGCAGCCATAGCACTAGTGGATGATAGAATGAGTACTGAAGGAACTGGTCTTCCATTTGGACTCAGCAACAATCTTCTTGGTTGGATTCTTTTTGGCGTGTTTGGTCTTATTTGGGCTCTCTATTTTATTTATGCTTCAGGTCTTGATGAGGATGAAGAGTCTGGTTTGTCCCTTTAAATTGGTTGGAATGATGATGAATTATGGATTGTAGAAAAGTACTGAAGTTATTTTCTGTGATGATATGATATGATATGTATCTAAGTAATGACTTTTGTTGTTGATTATTACTACCTCTTCTTTCCTTTAAACT
TARGET=617,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03046 Ps_contig_mira-and-tgicl-ass_franssen_28340
SEQUENCE=AATCAATCAACAACAAAAGTCATTACTTAGATACATATCATATCATATCATCACAGAAATAACTTCAGTACTTTTCTACAATCCATAATTCATCATCATTCCAACCAATTTAAAGGGACAAACCAGACTCTTCATCCTCATCAAGACCTGAAGCATAAATAAATAGAGAGCCCAAATAAGACCAAACACGCCAAAAGAATCCAACCAAGAAGATTGTTGCTGAAGTAACCCAAAAATGGAAGACCAGTTTCCTTCAGTACTCATTC
TARGET=34,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03047 Ps_contig_mira-and-tgicl-ass_franssen_28343
SEQUENCE=ACAACTCCTCAATAACATCTTCATTGAAAATTTTCTTTCTTTCCCTTTTGAATGCTTCTCTTACCTCAGTTTGTGCTTTTGCTAATACACTTGGGTTTCTTATCATTTCTGTCA
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03048 Ps_contig_mira-and-tgicl-ass_franssen_28344
SEQUENCE=ACAACTCCTCAATAACATCTTCATTGTAAAATTTTCTTTCTTTCCCTTTGAATGCTTCTCTTACCTCAGTTTGTGCTTTTGCTAATACACTTGGGTTTCTTATCATTTCTGTACTAAGTAA
TARGET=33,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03049 Ps_contig_mira-and-tgicl-ass_franssen_28346
SEQUENCE=CAACTCCTCAATAACATCTTCATTGAAATTTCTTTCTTTCCCTTTGAATGCTTCTCTTACCTCAGTTTGTGCTTTTGCTAATACACTTGGGTTTCTTATCATTTCTGTCA
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03050 Ps_contig_mira-and-tgicl-ass_franssen_28352
SEQUENCE=AAGCAGTGGTATCAACGGCAGAGTGGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAACTTAAACCCACCTGCTGGCTAATTATTGTTGGATATAACATGTGTAANGATGNGTTGACTAACTTTAGGTTATTTG
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8550
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.724641
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=TCCAACAATAATTAGCCAGCAG
PRIMER_LEFT=48,22
PRIMER_RIGHT=180,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=59.275
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=0.8550
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.724641
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=TCCAACAATAATTAGCCAGCAG
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=180,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.275
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=1.1881
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=1.057707
PRIMER_LEFT_2_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_2_SEQUENCE=ATAATTAGCCAGCAGGTGGGTT
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=173,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=61.058
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=1.1881
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=1.057707
PRIMER_LEFT_3_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_3_SEQUENCE=ATAATTAGCCAGCAGGTGGGTT
PRIMER_LEFT_3=47,22
PRIMER_RIGHT_3=173,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=61.058
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=1.2413
PRIMER_LEFT_4_PENALTY=0.516652
PRIMER_RIGHT_4_PENALTY=0.724641
PRIMER_LEFT_4_SEQUENCE=ATAGCTCAACAGTGGGAGGATG
PRIMER_RIGHT_4_SEQUENCE=TCCAACAATAATTAGCCAGCAG
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=180,22
PRIMER_LEFT_4_TM=60.517
PRIMER_RIGHT_4_TM=59.275
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=131
=
PRIMER_SEQUENCE_ID=SSR_03051 Ps_contig_mira-and-tgicl-ass_franssen_28353
SEQUENCE=TCGGGGATGAACCAAAACGACATCGTTTCTTGACAGTGTTCCAAATTATTTCAAGGTACACAAACCCTTTTTNTACGCCTTTGCAAGCAGAAAATTATTCTAATCTCATCAGTAGCGAACACTTAAAGGATCATCGTAGTTTTCATTCCCCCAATACCTGTTACAGATTCAGATTTACATATGGCGACCGAAACGAAGGCTGTTACCGAGGATGTCAAGATCGATCTCTTCGAAGACGACGATGAGTTCGAAGAGTTTGAAATCAATGAAGAGTGGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTATTATTGTTTGATATAACATGTGTAATGAATGAGTTGACTAACGTTTAGGTTATTTGGAGGTTTACGTTTACAACCCTTTAACCCTTAACTAAAGTTAAAACGGTTAAA
TARGET=328,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0492
PRIMER_LEFT_PENALTY=0.048460
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=AGTTTTCATTCCCCCAATACCT
PRIMER_RIGHT_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT=137,22
PRIMER_RIGHT=413,22
PRIMER_LEFT_TM=59.952
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=277
PRIMER_PAIR_PENALTY_1=0.0556
PRIMER_LEFT_1_PENALTY=0.054900
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=TCATTCCCCCAATACCTGTTAC
PRIMER_RIGHT_1_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT_1=142,22
PRIMER_RIGHT_1=413,22
PRIMER_LEFT_1_TM=59.945
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.0698
PRIMER_LEFT_2_PENALTY=0.069038
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=CGTAGTTTTCATTCCCCCAATA
PRIMER_RIGHT_2_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT_2=134,22
PRIMER_RIGHT_2=413,22
PRIMER_LEFT_2_TM=60.069
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.1027
PRIMER_LEFT_3_PENALTY=0.101938
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=ATTTACATATGGCGACCGAAAC
PRIMER_RIGHT_3_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT_3=172,22
PRIMER_RIGHT_3=413,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.1293
PRIMER_LEFT_4_PENALTY=0.048460
PRIMER_RIGHT_4_PENALTY=0.080887
PRIMER_LEFT_4_SEQUENCE=AGTTTTCATTCCCCCAATACCT
PRIMER_RIGHT_4_SEQUENCE=ACAATAATAGCCAGCAGGTGGT
PRIMER_LEFT_4=137,22
PRIMER_RIGHT_4=423,22
PRIMER_LEFT_4_TM=59.952
PRIMER_RIGHT_4_TM=59.919
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_03052 Ps_contig_mira-and-tgicl-ass_franssen_28354
SEQUENCE=TTTCCTAGANGCCAACAAATTACTATAGAANCACAGAACATAATTAGAAAAACAAAAACAAGTATYACAATAACTTCTATGAAGATGTTTAAAAGAAATAACATGTGAATTATGTCCTCGTGTTCTGTTCTTCTTACTCACATTTCCTTCCTAACACATCATAGTTTAAGTTCTTATGTAGGTAAGTAAACACCTCAAATACCTAAGTTGCAACTCATTCATTACACATGTTATATCAAACAATAATAGCCAGCAGGTGGTTTAATTCTTCTCTTGTTAGTTGTCTTCTCGAGCTCCCTTCTCAGCTGAACTGAGAAATCGTCACTAACATCATCATCATCCCAATCATCCTCCCACTGTTGAGCTATTTCTTTACCTTCCTCCTTGTCATCCCACTCTGTCGTTGATACCA
TARGET=329,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03053 Ps_contig_mira-and-tgicl-ass_franssen_28356
SEQUENCE=GCGGGGCTTNYNTGGCAAGCAGAAAATTATTCTAATCTCATCAGTAGCGAACACTTAAAGGATCATCGTAGTTTTCATTCCCCCAATACCTGATTCAGATTTACATATGGCGACCGAAACGAAGGCTGTTACCGAGGATGTCAAGATCGATCTCTTCGAAGACGACGATGAGTTCGAAAACTTTGGTATCAACGCAGAGTGGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTATTATTGTTTGATATAACATGTGTAATGAATGAGTTGCAACTTAGGTATTTGAGGTGTTTACTTACCTACATAAGAACTTAAACTATGATGTGTTAGGAAGGAAATGTGAGTAAGAAGAACAGAACACGAGGACATAATTCACATGTTATTTCTTTTAAACATCTTCATAGAAGTTATTGTGTACTTGTTTTTGTTTTTCAAATTTATGTTCTGTGAATTCTTATAGAATTTGTTTGGGTCATCTGGCTTTACTAAACTCTGCTTTCTACACTATTGTTGTTTTACTATGGGCGTTAAATTTAGTTAATTAGTTTTGGTAGGTTTTTGGGTTAGGTTAGGT
TARGET=254,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03054 Ps_contig_mira-and-tgicl-ass_franssen_28357
SEQUENCE=GGGGATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTATTATTGTTGATATAACATGTAGTAATGAATGAGTTGACTAAACGTTTAGGTTATTTGGAGGTTGTTTAACTTACCCTACAATAAAGTAAA
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1311
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT=25,22
PRIMER_RIGHT=140,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=0.1311
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=140,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.2112
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.080887
PRIMER_LEFT_2_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_2_SEQUENCE=ACAATAATAGCCAGCAGGTGGT
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=150,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=59.919
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.2112
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.080887
PRIMER_LEFT_3_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_3_SEQUENCE=ACAATAATAGCCAGCAGGTGGT
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=150,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=59.919
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=0.4616
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.331254
PRIMER_LEFT_4_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_4_SEQUENCE=CAACAATAATAGCCAGCAGGTG
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=152,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.669
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_03055 Ps_contig_mira-and-tgicl-ass_franssen_28358
SEQUENCE=AGTGGTATCAACGCAGAGTGGGGGAATGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGAGCTCGAGAGCACTACAGAGAAGAATTAAACCACCTGCTGGCTATTATTGTTGATATAACATGTGTAATGAATGAGTTGCCAACTTTAGGTTATTTGGAGGTTGTTTACCTAACCTTTAACCCTAACAATTAA
TARGET=76,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1311
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT=46,22
PRIMER_RIGHT=161,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=0.1311
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_1_SEQUENCE=CCAGCAGGTGGTTTAATTCTTC
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=161,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.2112
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.080887
PRIMER_LEFT_2_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_2_SEQUENCE=ACAATAATAGCCAGCAGGTGGT
PRIMER_LEFT_2=46,22
PRIMER_RIGHT_2=171,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=59.919
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.2112
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.080887
PRIMER_LEFT_3_SEQUENCE=GAAATAGCTCAACAGTGGGAGG
PRIMER_RIGHT_3_SEQUENCE=ACAATAATAGCCAGCAGGTGGT
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=171,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=59.919
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=0.4616
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.331254
PRIMER_LEFT_4_SEQUENCE=AAATAGCTCAACAGTGGGAGGA
PRIMER_RIGHT_4_SEQUENCE=CAACAATAATAGCCAGCAGGTG
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=173,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.669
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_03056 Ps_contig_mira-and-tgicl-ass_franssen_28359
SEQUENCE=GGGGATTGACAAGGAGGAAGGTAAAGAAATAGCTCAACAGTGGGAGGATGATTGGGATGATGATGATGTTAGTGACGATTTCTCAGTTCAGCTGAGAAGGGANCTCGAGAGCACTACAGAGAAGAATTAAACCCACCTGCTGGCTTATTAYTGTTGATATAACATGTAGTAATGAATGGAGTTGACTAACGTTAGGTTATTTGNAGGTTNTTTACTACCTANATAAGTAA
TARGET=56,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03057 Ps_contig_mira-and-tgicl-ass_franssen_28367
SEQUENCE=TTTATTGTGAGCCTTAATCACAAAGTAAGTAACAATTGAAAGAAACAAACAACTAGAGAAGCAAAACAGTTGACACACTCATATTCCAAATTAAAAGCTTGAATTNGACAACCAAATAAGAAAAGAACCAAAGAAAACAAAATTGTTTGCAAGCAAGCAAGCAGATCTCAAATATCAAACTTGTGATCCAACATAGATAATAGTTTTTATTTTTGCAGAGTTCACAACAACATAAATATGTTGTCAACATTTTATTCTATCGCGGCGGAGGATTAAAAGTCGACAATCTTTTTCCCTTTGAAGCATTCCACAATTTCCTTGAGACTGATTTCCAAAGGAATAAACTCAATTCCCAAACTCTTAGCCTTTTCCTTGGAAATCTGATATTTTGGCTTGTATGGCTCATCATTCTCACACTTGTCTGAAATTTGTAATGTTGGATACAAATCGCGTAAAACCTTAGCAAGTTCTGAACAGTGTAAGACTCTTTCACACAAAAGATATCTTCCACTAGCTGAAGCAATCTCATATGCGTTAATATGGGCGTTGGCAACATCTTTCACATTCACCCATCCATAAACATAATTTGGGAAAGGTTTACCATTTATTATGTCTATAAGTGGTTGAACACTTTCGTTAACCTCATGTTGTAAGAGAGGACCAACAACCATCGTTGGGTTCATAACAACCATGTCAATCTTGTTTTCATTTACAAATTCCCAGGCAGCAACCTCAGCCAAAGTCTTTGAAAGTGGGTACCATAACTGTAATTCCCTACAGAAATCTGGATCTGAAAACCATGTCTCATTAATTATGACTTGTGGATTCTTGGGCCTTGTGCTTATTGAAACTGCAGCAATAGAAGAAGTTAAGACAACCCGTTTCACAGATGGTGATTTCGCACATGATCTAAGAACATTAAGAGTTCCCTTCACCGCCGGATCAATCAACTCAGCCTGTGGGTTGTCTGCGGTACAACTAACAGGTGAAGCAGTATGAAAGACACCGTCACATCCCTTGACAGCAGTGTCAAAGGAACCTTGTTCCAACAGATCTGCTTTGAAGAGTTGCAACCTCTCCTTTGCACCATCAAGTTTAAACAAGTGGTCCACCCTTTTAGCATTACCTGCTTCCCGAACGGTCGCTCTCACAGTATAACCACGTTGGAGGAGAAACTTAATAATCCATGAAGCAATAAAACCAGAACCACCTGTCACACACACCACCTTTCCTTTTCCACTCATCATCCTTCCCTCCGT
TARGET=149,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6234
PRIMER_LEFT_PENALTY=1.520959
PRIMER_RIGHT_PENALTY=0.102490
PRIMER_LEFT_SEQUENCE=AGCAAAACAGTTGACACACTCA
PRIMER_RIGHT_SEQUENCE=CCTTTGGAAATCAGTCTCAAGG
PRIMER_LEFT=59,22
PRIMER_RIGHT=337,22
PRIMER_LEFT_TM=58.479
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=1.8098
PRIMER_LEFT_1_PENALTY=1.520959
PRIMER_RIGHT_1_PENALTY=0.288804
PRIMER_LEFT_1_SEQUENCE=AGCAAAACAGTTGACACACTCA
PRIMER_RIGHT_1_SEQUENCE=TCAGTCTCAAGGAAATTGTGGA
PRIMER_LEFT_1=59,22
PRIMER_RIGHT_1=327,22
PRIMER_LEFT_1_TM=58.479
PRIMER_RIGHT_1_TM=59.711
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=2.0037
PRIMER_LEFT_2_PENALTY=1.520959
PRIMER_RIGHT_2_PENALTY=0.482784
PRIMER_LEFT_2_SEQUENCE=AGCAAAACAGTTGACACACTCA
PRIMER_RIGHT_2_SEQUENCE=CTCAAGGAAATTGTGGAATGCT
PRIMER_LEFT_2=59,22
PRIMER_RIGHT_2=322,22
PRIMER_LEFT_2_TM=58.479
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=264
PRIMER_PAIR_PENALTY_3=2.0193
PRIMER_LEFT_3_PENALTY=1.520959
PRIMER_RIGHT_3_PENALTY=0.498341
PRIMER_LEFT_3_SEQUENCE=AGCAAAACAGTTGACACACTCA
PRIMER_RIGHT_3_SEQUENCE=ATTGTCGACTTTTAATCCTCCG
PRIMER_LEFT_3=59,22
PRIMER_RIGHT_3=286,22
PRIMER_LEFT_3_TM=58.479
PRIMER_RIGHT_3_TM=59.502
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=2.0384
PRIMER_LEFT_4_PENALTY=1.935944
PRIMER_RIGHT_4_PENALTY=0.102490
PRIMER_LEFT_4_SEQUENCE=GAGAAGCAAAACAGTTGACACA
PRIMER_RIGHT_4_SEQUENCE=CCTTTGGAAATCAGTCTCAAGG
PRIMER_LEFT_4=55,22
PRIMER_RIGHT_4=337,22
PRIMER_LEFT_4_TM=58.064
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_03058 Ps_contig_mira-and-tgicl-ass_franssen_28370
SEQUENCE=AATNTTTTNACYTACTTTGGTTACTTCTTCAATATCATACCTTACATGCCATTCATACATGTGCATATAATATCATTTATTCAGCACAATGTAGTTTACATAAGAAAGAAAGAAAATCAAAGCAGAACGCTATCAGATCATCACACCAGAAAACACACTGATTAAAATTCCCAAAGACACAACAGATACTATTTTTTTTATAGAAGTAATTCTGAATCATGGAAAATTTTAAATCCTTGATTCACTTCAATATTCACTAGCTGATTCTTTCCATCCACCACAGCTCTCCTCTTCATTGTAACCCTGTTCTTCATTTCTCTCAGGTGTAAAAGTGAGAGCCACTTTAATCTCACCACAATATTCTTCTTCCTTCACAAGTTTGTAAACACTCTCTGGAAGACTTCCCTCTTCAAACACAGCATCCAAAGGAATTATTGCTTCACCAAGATTATCATCATTACTAAACTTGTCCTTTTCCATCACCCTTAGATTGAGTTCAGAAGCAGAATCAGAAATAGTGAAAAGAAAGGTTTCATTCCACTGAGGATTGGATCCAGCACCTTGTTGCACATTACTCTTGTGTTCTTGTGCTCTGTAAGTAAGGATCACATAAGGATCTATGCTAGAGAGAAAATCATTGTCATCGAGACCTTTGGCACTGATCAAAACGACTTCAAGAGTTCCACGAGGCTATTTTTTTTTACGAATTCTAAAGGAGTTTTGATTATGAATGGTTCTCTGTTGTTTTATGGTTGTTAAAGTTGAAGTAAAAGCTGAATTTGTTGTACCAGAAAATGCTTGAATCAGTTGGAATTTTCCTGGATCCAACTTTTTCCTTTTTTAAATATTGACTCCAACTTTTGCACAGTCTTTTTTTTCTTCTTGTAATC
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03059 Ps_contig_mira-and-tgicl-ass_franssen_28389
SEQUENCE=AATTAAATTGAGTATTTATTTCTATTTTGCTATAAATAAAATCATCTTCTAAGATATTTTCGATTTTTCTTTTGATCATTCACAAACTCCACACACACCGCTTTCTCTCTTTCTGTGCCTCACTCTCACTTGAGGTATCATTTAGCATCATATCTTTTGATACTCTTTCGTGGCCTAGCAATTCCATTCCTTCAAATATCTTCACGTATGTTTCCAAATCCAGATTCTCTTTTTCCTCTTTAATCTCTCTCTTCACCGTTTCTTCAATTATTCAACTTCAACTTCCGACAGAATGAGTTCTAAAGTAGAAACTAATGGTGGTGGTGCTTCTTTAGTTTCTGCTAAAGTTCCACCTTTGGCGAATCCATTGGCTGAAAAACCTGATGAAATTGCTTCTAACATCAGTTACCATGCTCAGTATAGTCCTCATTTTTCCCCTTTCAAGTTTCAGCTTGAACAAGCTTATTATGCAACTGCAGAGAGTGTTCGTGATCGTCTCATTCAGCAATGGAATGAAACATACTTACATTTTCACAAAGTTGATCCCAAGCAAACATACTACTTATCAATGGAGTTCCTTCAAGGTCGGGCTTTGACCAATGCTATTGGAAATCTCAATATCCAAGATGCATATGCTGATGCTTTGCGCAAATTCGGACTTGAACTTGAAGAAATAACAGAGCAGGAGAAGGACGCAGCACTAGGAAATGGTGGTCTTGGTAGGCTTGCTTCTTGCTTTTTGGATTCCATGGCAACACTTAATTTGCCTGCTTGGGGATACGGTTTGAGGTATCGGTACGGACTATTTAAGCAGATAATCGCAAAAGAAGGTCAGGAGGAAGTTGCTGAGGACTGGCTTGAGAAGTTTAGCCCTTGGGAAATTGTGAGACATGACGTTTTGTACCCGATCAGATTCTTTGGCCAGGTCGACGTTAACCCTGATGGAAGCCGACAATGGGTAGGCGGAGAAGTTATTCAAGCACTAGCTTATGATGTGCCAATTCCTGGATACCAGACCAAGAACACCATCAGTCTTCGCCTCTGGGAAGCGAAAGCATGCGCTGATGATTTTGATTTGTTTTTATTCAATGATGGGCAACTCGAATCTGCTTCAGTTCTTCACTCACAAGCGCAACAGATTTGCTCGGTTTTGTATCCCGGTGATGCCACAGAAGGCGGGAAACTCCTACGGCTGAAGCAGCAGTACTTTCTCTGCAGTGCATCACTCCAAGACATTATTTCCCGATTCAAGGAGAGGAGGCAAGGACCTTGGAACTGGTCTGAGTTCCCAACAAAGGTTGCTGTACAATTGAACGATACCCACCCAACCCTTTCAATACCGGAGTTGATGCGATTACTTATGGATGTCGAAGGGCTTGGATGGGATGAAGCATGGGCAGTGACATCAAAGACGGTTGCTTACACTAATCACACTGTCCTCCCTGAAGCGCTGGAGAAATGGTCTCAACCTGTTATGTGGAAACTGCTTCCTCGTCACATGGAAATCATAGAGGAAATCGATAGACGATTCATTTCATTGATAAGTAACACCCGATTGGATCTTGAGGACGAACTTCCGAACATGCGTATCCTGGACAATAATACCCAGAAGCCAGTAGTTCGGATGGCAAATTTGTGCGTTGTTTCTTCTCATACTGTGAATGGTGTTGCTCAGTTACACAGTGATATATTGAAGTCAGAATTATTTGCAAATTATGTTTCAATATGGCCAACAAAATTCCAAAATAAAACTAATGGCATTACGCCTCGAAGATGGATCAATTTCTGCAGTCCTGAGCTAAGCAGGATAATCACAAAGTGGTTAAAAACTGATAAATGGGTAACCAATCTTGACTTATTAACAGGTCTTCGTGAGTTTGCTGACAATGAAGATCTACAAGCAGAGTGGCTGTCTGCAAAGAGAGCTAATAAGCAGCGCTTAGCACAGTATGTTCTGCAAGTGACAGGGGAGAGCATTGACCCTGATAGTCTATTCGACATTCAAGTCAAGCGTATCCACGAATACAAGAGGCAGCTGCTAAATATTCTTGGTGTGATCTATAGATATAAAAAGTTAAAGGAGATGAGCCCTGAAGAACGGAAAAGCACAACTGCACGCACGGTCATGATTGGAGGAAAGGCATTTGCGACATACACAAATGCTAAACGGATAGTCAAGCTTGTTGATGATGTTGGTTCTGTTGTAAACAGTGATCCTGAAGTCAATAGCTACTTGAAGGTTGTGTTTGTACCAAATTACAATGTATCAGTGGCGGAGGTGCTTATCCCAGGGAGTGAGCTGTCGCAGCATATTAGCACTGCAGGAATGGAAGCAAGTGGCACAAGCAACATGAAATTTGCATTGAACGGGTGCCTCATAATCGGTACATTAGATGGAGCTAATGTTGAAATCCGGGAAGAGATTGGCGAAGAGAATTTTTTCCTCTTTGGTGCAACAGCAGATGAAGTCCCTCGACTCAGGAAGGAAAGAGAGAATGGACTGTTCAAGCCAGATCCTCGATTCGAAGAGGCAAAGAAGTTTATAAGGAGTGGGGTGTTTGGAAGCTACGACTATAACCCATTGCTTGATTCATTGGAAGGAAATTCTGGCTATGGTCGCGGGGATTACTTTCTGGTTGGGTATGACTTCCCAAGCTACATGGATGCTCAGGAAAAAGTAGATGAAGCATATCGTGATAAGAAAAGGTGGCTAAAAATGTCTATTTTAAGCACTGCTGGGAGTGGGAAGTTCAGCAGTGACAGGACAATTGCTCAGTATGCTAAGGAAATTTGGAACATTGAAGAATGCCGCGTACCATAACTTCAGCTTGCATCCAAGGCTCTGTATAATATTATAGCATTGAATCTAATGAGAAGTGTATAGTCATGAATAAAAACGAACATAACTTTCTATATTTGACTATAGTATGCTGTGTCGAGTTTCAACTGTGTTATTATTATTATTGTAGAGTGTCGTCTCTCTTAATCAATGCATCTGTTTCTATCTTATGAAAATTAAATTTGTAACATATTTTTAGTCTTCTAA
TARGET=2952,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3230
PRIMER_LEFT_PENALTY=0.046801
PRIMER_RIGHT_PENALTY=2.276218
PRIMER_LEFT_SEQUENCE=AAGTTCAGCAGTGACAGGACAA
PRIMER_RIGHT_SEQUENCE=CAGATGCATTGATTAAGAGAGACG
PRIMER_LEFT=2746,22
PRIMER_RIGHT=2997,24
PRIMER_LEFT_TM=59.953
PRIMER_RIGHT_TM=60.276
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=2.7169
PRIMER_LEFT_1_PENALTY=0.046801
PRIMER_RIGHT_1_PENALTY=2.670094
PRIMER_LEFT_1_SEQUENCE=AAGTTCAGCAGTGACAGGACAA
PRIMER_RIGHT_1_SEQUENCE=TGCATTGATTAAGAGAGACGACAC
PRIMER_LEFT_1=2746,22
PRIMER_RIGHT_1=2993,24
PRIMER_LEFT_1_TM=59.953
PRIMER_RIGHT_1_TM=60.670
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=2.7532
PRIMER_LEFT_2_PENALTY=0.046801
PRIMER_RIGHT_2_PENALTY=2.706391
PRIMER_LEFT_2_SEQUENCE=AAGTTCAGCAGTGACAGGACAA
PRIMER_RIGHT_2_SEQUENCE=GATGCATTGATTAAGAGAGACGAC
PRIMER_LEFT_2=2746,22
PRIMER_RIGHT_2=2995,24
PRIMER_LEFT_2_TM=59.953
PRIMER_RIGHT_2_TM=59.294
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=2.7647
PRIMER_LEFT_3_PENALTY=0.488446
PRIMER_RIGHT_3_PENALTY=2.276218
PRIMER_LEFT_3_SEQUENCE=GAAGTTCAGCAGTGACAGGACA
PRIMER_RIGHT_3_SEQUENCE=CAGATGCATTGATTAAGAGAGACG
PRIMER_LEFT_3=2745,22
PRIMER_RIGHT_3=2997,24
PRIMER_LEFT_3_TM=60.488
PRIMER_RIGHT_3_TM=60.276
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=253
PRIMER_PAIR_PENALTY_4=2.7872
PRIMER_LEFT_4_PENALTY=0.510985
PRIMER_RIGHT_4_PENALTY=2.276218
PRIMER_LEFT_4_SEQUENCE=ACCATAACTTCAGCTTGCATCC
PRIMER_RIGHT_4_SEQUENCE=CAGATGCATTGATTAAGAGAGACG
PRIMER_LEFT_4=2814,22
PRIMER_RIGHT_4=2997,24
PRIMER_LEFT_4_TM=60.511
PRIMER_RIGHT_4_TM=60.276
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_03060 Ps_contig_mira-and-tgicl-ass_franssen_28401
SEQUENCE=TGATGGTTCCTGCAATTGCCTCTGGACTAATTTGTGGGGAAGGACTATGGACTCTTCCGGCTGCTATTCTTGCTCTAGCAAAAATTATCCGCCTATATGCATGTAAATTTGTTTCTTACTTAAAACTTGACTCAACATATGAAGAGCTATTAGTCTTTAGTTTTTTACCTTTTTTTTTCTTTCTTTCTTTTGCACGTAGTTGTGTACTCTAGAAGCACACATACAAAACAAATTAATAAAATGAATTAGTTAAA
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.5451
PRIMER_LEFT_PENALTY=0.344527
PRIMER_RIGHT_PENALTY=4.200595
PRIMER_LEFT_SEQUENCE=TCTGGACTAATTTGTGGGGAAG
PRIMER_RIGHT_SEQUENCE=GCTTCTAGAGTACACAACTACGTGC
PRIMER_LEFT=20,22
PRIMER_RIGHT=215,25
PRIMER_LEFT_TM=60.345
PRIMER_RIGHT_TM=58.799
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=48.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=4.5451
PRIMER_LEFT_1_PENALTY=0.344527
PRIMER_RIGHT_1_PENALTY=4.200595
PRIMER_LEFT_1_SEQUENCE=CTCTGGACTAATTTGTGGGGAA
PRIMER_RIGHT_1_SEQUENCE=GCTTCTAGAGTACACAACTACGTGC
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=215,25
PRIMER_LEFT_1_TM=60.345
PRIMER_RIGHT_1_TM=58.799
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=48.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=4.5654
PRIMER_LEFT_2_PENALTY=0.364827
PRIMER_RIGHT_2_PENALTY=4.200595
PRIMER_LEFT_2_SEQUENCE=GCAATTGCCTCTGGACTAATTT
PRIMER_RIGHT_2_SEQUENCE=GCTTCTAGAGTACACAACTACGTGC
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=215,25
PRIMER_LEFT_2_TM=59.635
PRIMER_RIGHT_2_TM=58.799
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=48.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=4.8322
PRIMER_LEFT_3_PENALTY=0.631568
PRIMER_RIGHT_3_PENALTY=4.200595
PRIMER_LEFT_3_SEQUENCE=CTGCAATTGCCTCTGGACTAAT
PRIMER_RIGHT_3_SEQUENCE=GCTTCTAGAGTACACAACTACGTGC
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=215,25
PRIMER_LEFT_3_TM=60.632
PRIMER_RIGHT_3_TM=58.799
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=48.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=4.8915
PRIMER_LEFT_4_PENALTY=0.690888
PRIMER_RIGHT_4_PENALTY=4.200595
PRIMER_LEFT_4_SEQUENCE=TGCTCTAGCAAAAATTATCCGC
PRIMER_RIGHT_4_SEQUENCE=GCTTCTAGAGTACACAACTACGTGC
PRIMER_LEFT_4=70,22
PRIMER_RIGHT_4=215,25
PRIMER_LEFT_4_TM=60.691
PRIMER_RIGHT_4_TM=58.799
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=48.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_03061 Ps_contig_mira-and-tgicl-ass_franssen_28402
SEQUENCE=TGTATTGGTTTCCTGCAATTGCCTCTGGACTAATTTGTGGGGAAGGACTATGGACTCTTCCGGCTGCTATTCTTGCTCTAGCAAAATTAATCCGCCTATATGCATGAAATTTGTTTCTTACTTAAAACTGACTCAACATATGAAGAGCATTAGCTTAGTTTTTACCTTTTTTTTTCTTTCTTTCTTTTTGCGCGTAGTTGTGTACTTTGAAGCCACACATACAAAACTAATATAAATGATTGT
TARGET=173,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4657
PRIMER_LEFT_PENALTY=0.344527
PRIMER_RIGHT_PENALTY=0.121175
PRIMER_LEFT_SEQUENCE=TCTGGACTAATTTGTGGGGAAG
PRIMER_RIGHT_SEQUENCE=AAGTACACAACTACGCGCAAAA
PRIMER_LEFT=23,22
PRIMER_RIGHT=206,22
PRIMER_LEFT_TM=60.345
PRIMER_RIGHT_TM=59.879
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.4657
PRIMER_LEFT_1_PENALTY=0.344527
PRIMER_RIGHT_1_PENALTY=0.121175
PRIMER_LEFT_1_SEQUENCE=CTCTGGACTAATTTGTGGGGAA
PRIMER_RIGHT_1_SEQUENCE=AAGTACACAACTACGCGCAAAA
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=206,22
PRIMER_LEFT_1_TM=60.345
PRIMER_RIGHT_1_TM=59.879
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.4657
PRIMER_LEFT_2_PENALTY=0.344527
PRIMER_RIGHT_2_PENALTY=0.121175
PRIMER_LEFT_2_SEQUENCE=TCTGGACTAATTTGTGGGGAAG
PRIMER_RIGHT_2_SEQUENCE=AAAGTACACAACTACGCGCAAA
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=207,22
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=59.879
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.4657
PRIMER_LEFT_3_PENALTY=0.344527
PRIMER_RIGHT_3_PENALTY=0.121175
PRIMER_LEFT_3_SEQUENCE=CTCTGGACTAATTTGTGGGGAA
PRIMER_RIGHT_3_SEQUENCE=AAAGTACACAACTACGCGCAAA
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=207,22
PRIMER_LEFT_3_TM=60.345
PRIMER_RIGHT_3_TM=59.879
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=0.4860
PRIMER_LEFT_4_PENALTY=0.364827
PRIMER_RIGHT_4_PENALTY=0.121175
PRIMER_LEFT_4_SEQUENCE=GCAATTGCCTCTGGACTAATTT
PRIMER_RIGHT_4_SEQUENCE=AAGTACACAACTACGCGCAAAA
PRIMER_LEFT_4=14,22
PRIMER_RIGHT_4=206,22
PRIMER_LEFT_4_TM=59.635
PRIMER_RIGHT_4_TM=59.879
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_03062 Ps_contig_mira-and-tgicl-ass_franssen_28403
SEQUENCE=TATAGGGTTCCTGCAATTGCCTCTGGACTAATTTGTGGGGAAGGACTATGGACTCTTCCGGCTGCTATTCTTGCTCTAGACAAAAAATTAATCCGCCTATATGCATGAAATTTGTTTCTTACTTAAAACTTGACTCAACATATGAAGAGCATTAGCTTTAGTTTTTACCTTTTTTTTTTCTTTCTTTCTTTTTGCAGTAGTTTGTGTACTTTTGAAGCCACACATACAAAACAAATATAAAATGAT
TARGET=177,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3534
PRIMER_LEFT_PENALTY=0.344527
PRIMER_RIGHT_PENALTY=2.008825
PRIMER_LEFT_SEQUENCE=TCTGGACTAATTTGTGGGGAAG
PRIMER_RIGHT_SEQUENCE=GTGTGGCTTCAAAAGTACACAAAC
PRIMER_LEFT=21,22
PRIMER_RIGHT=222,24
PRIMER_LEFT_TM=60.345
PRIMER_RIGHT_TM=60.009
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=2.3534
PRIMER_LEFT_1_PENALTY=0.344527
PRIMER_RIGHT_1_PENALTY=2.008825
PRIMER_LEFT_1_SEQUENCE=CTCTGGACTAATTTGTGGGGAA
PRIMER_RIGHT_1_SEQUENCE=GTGTGGCTTCAAAAGTACACAAAC
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=222,24
PRIMER_LEFT_1_TM=60.345
PRIMER_RIGHT_1_TM=60.009
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=2.3737
PRIMER_LEFT_2_PENALTY=0.364827
PRIMER_RIGHT_2_PENALTY=2.008825
PRIMER_LEFT_2_SEQUENCE=GCAATTGCCTCTGGACTAATTT
PRIMER_RIGHT_2_SEQUENCE=GTGTGGCTTCAAAAGTACACAAAC
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=222,24
PRIMER_LEFT_2_TM=59.635
PRIMER_RIGHT_2_TM=60.009
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=2.6404
PRIMER_LEFT_3_PENALTY=0.631568
PRIMER_RIGHT_3_PENALTY=2.008825
PRIMER_LEFT_3_SEQUENCE=CTGCAATTGCCTCTGGACTAAT
PRIMER_RIGHT_3_SEQUENCE=GTGTGGCTTCAAAAGTACACAAAC
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=222,24
PRIMER_LEFT_3_TM=60.632
PRIMER_RIGHT_3_TM=60.009
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=2.7430
PRIMER_LEFT_4_PENALTY=0.734195
PRIMER_RIGHT_4_PENALTY=2.008825
PRIMER_LEFT_4_SEQUENCE=GTGGGGAAGGACTATGGACTCT
PRIMER_RIGHT_4_SEQUENCE=GTGTGGCTTCAAAAGTACACAAAC
PRIMER_LEFT_4=34,22
PRIMER_RIGHT_4=222,24
PRIMER_LEFT_4_TM=60.734
PRIMER_RIGHT_4_TM=60.009
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_03063 Ps_contig_mira-and-tgicl-ass_franssen_28408
SEQUENCE=TGTTATTTGGTTTTCCTTGTCAATTGCCTCTGGACTAATTTGTGGGGAGGGACTATGGACTCTTCCGGCTGCTATTCTTGCTCTAGCAAAAATTANCCGCCTATATGCATGAAATTTGTTCTTCTTAAAACTTGACTCAACATATGAAGAGCATTAGCTTAGTTTTACCTTTTTTTTCTTTCTTTCTTTTTGCACGTAGTTGTGTACTCTGAAGCACACATACAAAACAAATATAAAATGATGTTAAA
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1544
PRIMER_LEFT_PENALTY=0.062596
PRIMER_RIGHT_PENALTY=2.091795
PRIMER_LEFT_SEQUENCE=CTCTAGCAAAAATTANCCGCCT
PRIMER_RIGHT_SEQUENCE=TCAGAGTACACAACTACGTGCAAA
PRIMER_LEFT=80,22
PRIMER_RIGHT=211,24
PRIMER_LEFT_TM=59.937
PRIMER_RIGHT_TM=59.908
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.50
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=2.1544
PRIMER_LEFT_1_PENALTY=0.062596
PRIMER_RIGHT_1_PENALTY=2.091795
PRIMER_LEFT_1_SEQUENCE=CTCTAGCAAAAATTANCCGCCT
PRIMER_RIGHT_1_SEQUENCE=TTCAGAGTACACAACTACGTGCAA
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=212,24
PRIMER_LEFT_1_TM=59.937
PRIMER_RIGHT_1_TM=59.908
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.50
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=2.1601
PRIMER_LEFT_2_PENALTY=0.068302
PRIMER_RIGHT_2_PENALTY=2.091795
PRIMER_LEFT_2_SEQUENCE=TAATTTGTGGGGAGGGACTATG
PRIMER_RIGHT_2_SEQUENCE=TCAGAGTACACAACTACGTGCAAA
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=211,24
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=59.908
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=2.1601
PRIMER_LEFT_3_PENALTY=0.068302
PRIMER_RIGHT_3_PENALTY=2.091795
PRIMER_LEFT_3_SEQUENCE=TAATTTGTGGGGAGGGACTATG
PRIMER_RIGHT_3_SEQUENCE=TTCAGAGTACACAACTACGTGCAA
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=212,24
PRIMER_LEFT_3_TM=60.068
PRIMER_RIGHT_3_TM=59.908
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=178
PRIMER_PAIR_PENALTY_4=2.3453
PRIMER_LEFT_4_PENALTY=0.253482
PRIMER_RIGHT_4_PENALTY=2.091795
PRIMER_LEFT_4_SEQUENCE=TTGTCAATTGCCTCTGGACTAA
PRIMER_RIGHT_4_SEQUENCE=TCAGAGTACACAACTACGTGCAAA
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=211,24
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=59.908
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=196
=
PRIMER_SEQUENCE_ID=SSR_03064 Ps_contig_mira-and-tgicl-ass_franssen_28429
SEQUENCE=AGGGAAACTTTAAATTCTCAAAGCCCTAATTTTCTTCACTCTCTACTCTCTCCTCCACGACACTCCTCGGTAACAGTGAGTTACAATGGTTGAATCGCTCTTTGAAGATATTTTTAGAGTTGAGAAATTAAACCCAGATGACAAAAAATTGTTCGAGAAAGTTAATCGCATTGAAGCAAGAAGTGAAAGGTTTGATATGTTTATGCACTTAGACATCAATTCTGAGCTGTATCCGTTAAAAGAGGGACAGAAATTTTTTCTTAAGGTTGTTTCAACACTCAATCCTGATGGTACCCCTGATACTGGCTACTATAATCCGGGCAATCGGCAATCTTTGGCTGATAACTTTGAATATGTCATGTATGGAAAGCTCTACAGAGTAACAGAGAGTACTGGGCGTGAAAAAGCAGAGTTACTGATTTCATTTGGTGGGCTTCTGATGATGATGAAGGGAGATCACTCTCATTTCAACAAGTTTGAACTTGATCAAAGGTTGTTTCTTCTGATTAGGAAAGTATGAAGATCAAGTTAAAAACGTTTCTTACATCAATGTTTGGAAGGTTTGTTTACCGTTTGTAATACTAATGCGGAGTCTTCGCGATCCAATGATGTTTTAAAAACAAATAGTTGTACCTTGAAGTAACTTTTGGCAGAAATGATCAACAACAAAAAATTGTAATTCTGCCCTTAAAGTTTGATTTCAGACCAAGGATTGTAGCATGACTATCTACTTTGGATTAGTTTCTAGTAAATGAATTTTAGTTAAATAATGATCACACAAAATGTTATTATGGNAGGTTTCTTGGTTCTTT
TARGET=438,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2804
PRIMER_LEFT_PENALTY=0.064105
PRIMER_RIGHT_PENALTY=0.216250
PRIMER_LEFT_SEQUENCE=TAACAGAGAGTACTGGGCGTGA
PRIMER_RIGHT_SEQUENCE=GCGAAGACTCCGCATTAGTATT
PRIMER_LEFT=380,22
PRIMER_RIGHT=598,22
PRIMER_LEFT_TM=59.936
PRIMER_RIGHT_TM=59.784
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.5112
PRIMER_LEFT_1_PENALTY=0.294975
PRIMER_RIGHT_1_PENALTY=0.216250
PRIMER_LEFT_1_SEQUENCE=GAGAGTACTGGGCGTGAAAAAG
PRIMER_RIGHT_1_SEQUENCE=GCGAAGACTCCGCATTAGTATT
PRIMER_LEFT_1=385,22
PRIMER_RIGHT_1=598,22
PRIMER_LEFT_1_TM=60.295
PRIMER_RIGHT_1_TM=59.784
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=0.5112
PRIMER_LEFT_2_PENALTY=0.294975
PRIMER_RIGHT_2_PENALTY=0.216250
PRIMER_LEFT_2_SEQUENCE=AGAGAGTACTGGGCGTGAAAAA
PRIMER_RIGHT_2_SEQUENCE=GCGAAGACTCCGCATTAGTATT
PRIMER_LEFT_2=384,22
PRIMER_RIGHT_2=598,22
PRIMER_LEFT_2_TM=60.295
PRIMER_RIGHT_2_TM=59.784
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=0.5811
PRIMER_LEFT_3_PENALTY=0.364827
PRIMER_RIGHT_3_PENALTY=0.216250
PRIMER_LEFT_3_SEQUENCE=GGCAATCTTTGGCTGATAACTT
PRIMER_RIGHT_3_SEQUENCE=GCGAAGACTCCGCATTAGTATT
PRIMER_LEFT_3=326,22
PRIMER_RIGHT_3=598,22
PRIMER_LEFT_3_TM=59.635
PRIMER_RIGHT_3_TM=59.784
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.7386
PRIMER_LEFT_4_PENALTY=0.064105
PRIMER_RIGHT_4_PENALTY=0.674496
PRIMER_LEFT_4_SEQUENCE=TAACAGAGAGTACTGGGCGTGA
PRIMER_RIGHT_4_SEQUENCE=ACGGTAAACAAACCTTCCAAAC
PRIMER_LEFT_4=380,22
PRIMER_RIGHT_4=572,22
PRIMER_LEFT_4_TM=59.936
PRIMER_RIGHT_4_TM=59.326
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_03065 Ps_contig_mira-and-tgicl-ass_franssen_28430
SEQUENCE=GGGCGTTGAAAAAGCAGAGTTACTGATTTCATTTGGTGGGCTTCTGATGATGATGAAGGGAGATCACTCTCATTTCAACAAGTTTGAACTTGATCAAAGGTTGTTTCTTCTGATTAGGAAAGTATGAAGATCAAGTTAAAAACGTTTCTTACATCAATGTTTGGAAGGTTTGTTTACCGTTTGTAATACTAATGCGGAGTCTTCGCGATCCAATGATGTTTTAAAAACAAATGTTGTACCTTGAAGTAACCTTTGGCAGAAATGATNNACAACAAAAAATTAGTAATTACTTGCCCTTAAACGTTTTAGTATTTCTAGACCAAGGATTGTAGCATGACTATCTACTTTGGTAGTTAGTTTACTTTACGTTAAAATTAAGTAATTTTAAAGTTT
TARGET=45,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5121
PRIMER_LEFT_PENALTY=0.295843
PRIMER_RIGHT_PENALTY=0.216250
PRIMER_LEFT_SEQUENCE=GGCGTTGAAAAAGCAGAGTTAC
PRIMER_RIGHT_SEQUENCE=GCGAAGACTCCGCATTAGTATT
PRIMER_LEFT=1,22
PRIMER_RIGHT=205,22
PRIMER_LEFT_TM=60.296
PRIMER_RIGHT_TM=59.784
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.6056
PRIMER_LEFT_1_PENALTY=0.295843
PRIMER_RIGHT_1_PENALTY=0.309780
PRIMER_LEFT_1_SEQUENCE=GGCGTTGAAAAAGCAGAGTTAC
PRIMER_RIGHT_1_SEQUENCE=CTGCCAAAGGTTACTTCAAGGT
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=258,22
PRIMER_LEFT_1_TM=60.296
PRIMER_RIGHT_1_TM=59.690
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.9291
PRIMER_LEFT_2_PENALTY=0.295843
PRIMER_RIGHT_2_PENALTY=0.633248
PRIMER_LEFT_2_SEQUENCE=GGCGTTGAAAAAGCAGAGTTAC
PRIMER_RIGHT_2_SEQUENCE=TCTGCCAAAGGTTACTTCAAGG
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=60.296
PRIMER_RIGHT_2_TM=60.633
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.9377
PRIMER_LEFT_3_PENALTY=0.721451
PRIMER_RIGHT_3_PENALTY=0.216250
PRIMER_LEFT_3_SEQUENCE=TACTGATTTCATTTGGTGGGCT
PRIMER_RIGHT_3_SEQUENCE=GCGAAGACTCCGCATTAGTATT
PRIMER_LEFT_3=20,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=60.721
PRIMER_RIGHT_3_TM=59.784
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=0.9703
PRIMER_LEFT_4_PENALTY=0.295843
PRIMER_RIGHT_4_PENALTY=0.674496
PRIMER_LEFT_4_SEQUENCE=GGCGTTGAAAAAGCAGAGTTAC
PRIMER_RIGHT_4_SEQUENCE=ACAAACGGTAAACAAACCTTCC
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=183,22
PRIMER_LEFT_4_TM=60.296
PRIMER_RIGHT_4_TM=59.326
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_03066 Ps_contig_mira-and-tgicl-ass_franssen_28431
SEQUENCE=TACTTGTATTTCATTTGGTGGGCTTCTGATGATGATGAAGGGAGATCACTCTCATTTCAACAAGTTTGAACTTGATCAAAGGTTGTTTCTTCTGATTAAGGAAAGTATGAAGATCAAAGTTAAAAACGTTTCTTACATCAATTGTTGGAAGGTTTTGTTTTACCCGTTTGTAATACTAATGGCGGATCTTCGCGATCCAATGTATTAGTTTTAAAAAACAATGTTGTCCTGAAGTACTT
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5804
PRIMER_LEFT_PENALTY=0.115769
PRIMER_RIGHT_PENALTY=0.464628
PRIMER_LEFT_SEQUENCE=ACTTGTATTTCATTTGGTGGGC
PRIMER_RIGHT_SEQUENCE=AACGGGTAAAACAAAACCTTCC
PRIMER_LEFT=1,22
PRIMER_RIGHT=167,22
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=60.465
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.7013
PRIMER_LEFT_1_PENALTY=0.236697
PRIMER_RIGHT_1_PENALTY=0.464628
PRIMER_LEFT_1_SEQUENCE=CTTGTATTTCATTTGGTGGGCT
PRIMER_RIGHT_1_SEQUENCE=AACGGGTAAAACAAAACCTTCC
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=167,22
PRIMER_LEFT_1_TM=60.237
PRIMER_RIGHT_1_TM=60.465
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=166
PRIMER_PAIR_PENALTY_2=0.8813
PRIMER_LEFT_2_PENALTY=0.115769
PRIMER_RIGHT_2_PENALTY=0.765552
PRIMER_LEFT_2_SEQUENCE=ACTTGTATTTCATTTGGTGGGC
PRIMER_RIGHT_2_SEQUENCE=CTAATACATTGGATCGCGAAGA
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=207,22
PRIMER_LEFT_2_TM=60.116
PRIMER_RIGHT_2_TM=59.234
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=1.0022
PRIMER_LEFT_3_PENALTY=0.236697
PRIMER_RIGHT_3_PENALTY=0.765552
PRIMER_LEFT_3_SEQUENCE=CTTGTATTTCATTTGGTGGGCT
PRIMER_RIGHT_3_SEQUENCE=CTAATACATTGGATCGCGAAGA
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=207,22
PRIMER_LEFT_3_TM=60.237
PRIMER_RIGHT_3_TM=59.234
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=1.0882
PRIMER_LEFT_4_PENALTY=0.623595
PRIMER_RIGHT_4_PENALTY=0.464628
PRIMER_LEFT_4_SEQUENCE=GTATTTCATTTGGTGGGCTTCT
PRIMER_RIGHT_4_SEQUENCE=AACGGGTAAAACAAAACCTTCC
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=167,22
PRIMER_LEFT_4_TM=59.376
PRIMER_RIGHT_4_TM=60.465
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_03067 Ps_contig_mira-and-tgicl-ass_franssen_28432
SEQUENCE=ACCGGGGATACTGGCGTAGGAACAGCAAAGTTAGGGTTTGTTATTCTTCTTCTTCTCCTCCATCATTCATCATGGGTCGTTTGCACTCAAAAGGTAAGGGTATTTCCAGTTCAGCTCTTCCTTACAGGAGAACAGCTCCATCTTGGCTCAAGATCTCTTCCCAGGATGTTGATGAAACCATCTGCAAGTTTGCCAAGAAGTTACNCATCACAATTGGTNTTATTCTTCGT
TARGET=44,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1089
PRIMER_LEFT_PENALTY=0.035021
PRIMER_RIGHT_PENALTY=0.073866
PRIMER_LEFT_SEQUENCE=GGAACAGCAAAGTTAGGGTTTG
PRIMER_RIGHT_SEQUENCE=ACATCCTGGGAAGAGATCTTGA
PRIMER_LEFT=18,22
PRIMER_RIGHT=168,22
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=0.2391
PRIMER_LEFT_1_PENALTY=0.035021
PRIMER_RIGHT_1_PENALTY=0.204119
PRIMER_LEFT_1_SEQUENCE=GGAACAGCAAAGTTAGGGTTTG
PRIMER_RIGHT_1_SEQUENCE=CATCCTGGGAAGAGATCTTGAG
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=167,22
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=60.204
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=150
PRIMER_PAIR_PENALTY_2=0.2490
PRIMER_LEFT_2_PENALTY=0.175100
PRIMER_RIGHT_2_PENALTY=0.073866
PRIMER_LEFT_2_SEQUENCE=CGTAGGAACAGCAAAGTTAGGG
PRIMER_RIGHT_2_SEQUENCE=ACATCCTGGGAAGAGATCTTGA
PRIMER_LEFT_2=14,22
PRIMER_RIGHT_2=168,22
PRIMER_LEFT_2_TM=60.175
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.3376
PRIMER_LEFT_3_PENALTY=0.035021
PRIMER_RIGHT_3_PENALTY=0.302618
PRIMER_LEFT_3_SEQUENCE=GGAACAGCAAAGTTAGGGTTTG
PRIMER_RIGHT_3_SEQUENCE=ACTTCTTGGCAAACTTGCAGAT
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=200,22
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=60.303
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.3792
PRIMER_LEFT_4_PENALTY=0.175100
PRIMER_RIGHT_4_PENALTY=0.204119
PRIMER_LEFT_4_SEQUENCE=CGTAGGAACAGCAAAGTTAGGG
PRIMER_RIGHT_4_SEQUENCE=CATCCTGGGAAGAGATCTTGAG
PRIMER_LEFT_4=14,22
PRIMER_RIGHT_4=167,22
PRIMER_LEFT_4_TM=60.175
PRIMER_RIGHT_4_TM=60.204
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_03068 Ps_contig_mira-and-tgicl-ass_franssen_28435
SEQUENCE=CGGGGGTTGATACTGGCGTAGGAACAGCAAAGTTAGGGTTTGTTATTCTTCTTCTTCTCCTCCATCATTCATCATGGGTCGTTTGCACTCAAAAGGTAAGGGTATTTCCAGTTCAGCTCTTCCTTACAGGAGAACAGCTCCATCTTGGCTCAAGATCTCTTCCCAGGATGTTGATGAAACCATCTGCAAGTTTGCCAAGAAAGGTTTAACTCCATCACAAATTGGTGTTATTCTTCGTGACTCCCATGGAATTGCTCAGGTTAAGAGTGTAACTGGAAGCAAGATTCTGCGCATATTGAAGGCTCATGGTCTTGCTCCTGAAATTCCTGAAGATCTGTATCACTTGATCAAGAAGGCTGTCTCAATCAGGAAGCATTTGGAGAGGAACAGGAAGGACAAGGATTCTAAGTTCAGGCTTATTTTGGTTGAGAGCAGGATCCATCGTCTTGCTCGTTACTACAAGAAGACAAAGAAGCTTCCACCAGTATGGAAATATGAATCAACAACTGCCAGTACCCTTGTTGCTTAAGAGAAGATTGGAGAATGAATTAGTTTGTTGAGTTTTTAAAATTTTGGAATATTTGAAGTTTATGTTAAGACTCGATTATGATTTTGGATCTTCTTTCTTTATTTGATATACTTCTTTTCTCTACTCTAATAATGGTTTTAAATTTGGTACCTCAAAATAGAACTTAATTAGTCAAATAAACTAGTCATGCATTTGCCTGTTGGGTTTTTATTTTAACTATAGTCTTCGATGCYAAATTCATTGAGTCTATCGTTGGAATTTGTTTGTCTTTCACTAATATGAGAATCACAAGTGTCTTAGTTAAAA
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03069 Ps_contig_mira-and-tgicl-ass_franssen_28436
SEQUENCE=GGGTTGATACTGGCGTAGGAACAGCAAAGTTAGGGTTTGTTATTCTTCTTCTTCTCCTCCATCATTCATCATGGGTCGTTTGCACTCAAAAGGTAAGGGTATTTCCAGTTCAGCTCTTCCTTACAGGAGAACAGCTCCATCTTGGCTCAAGATCTCTTCCAGGATGTTGATGAAACCATCTGCTAAGTTTGACCAAAAGGTAAAAGGTTTAACCTCCATCAACAAATTGGTNTTATTCTT
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3646
PRIMER_LEFT_PENALTY=0.035021
PRIMER_RIGHT_PENALTY=0.329550
PRIMER_LEFT_SEQUENCE=GGAACAGCAAAGTTAGGGTTTG
PRIMER_RIGHT_SEQUENCE=TTCATCAACATCCTGGAAGAGA
PRIMER_LEFT=17,22
PRIMER_RIGHT=173,22
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=59.670
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.3837
PRIMER_LEFT_1_PENALTY=0.035021
PRIMER_RIGHT_1_PENALTY=0.348633
PRIMER_LEFT_1_SEQUENCE=GGAACAGCAAAGTTAGGGTTTG
PRIMER_RIGHT_1_SEQUENCE=CATCCTGGAAGAGATCTTGAGC
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=165,22
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=60.349
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.5046
PRIMER_LEFT_2_PENALTY=0.175100
PRIMER_RIGHT_2_PENALTY=0.329550
PRIMER_LEFT_2_SEQUENCE=CGTAGGAACAGCAAAGTTAGGG
PRIMER_RIGHT_2_SEQUENCE=TTCATCAACATCCTGGAAGAGA
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=173,22
PRIMER_LEFT_2_TM=60.175
PRIMER_RIGHT_2_TM=59.670
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=0.5237
PRIMER_LEFT_3_PENALTY=0.175100
PRIMER_RIGHT_3_PENALTY=0.348633
PRIMER_LEFT_3_SEQUENCE=CGTAGGAACAGCAAAGTTAGGG
PRIMER_RIGHT_3_SEQUENCE=CATCCTGGAAGAGATCTTGAGC
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=165,22
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=60.349
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=153
PRIMER_PAIR_PENALTY_4=0.6375
PRIMER_LEFT_4_PENALTY=0.307953
PRIMER_RIGHT_4_PENALTY=0.329550
PRIMER_LEFT_4_SEQUENCE=GCGTAGGAACAGCAAAGTTAGG
PRIMER_RIGHT_4_SEQUENCE=TTCATCAACATCCTGGAAGAGA
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=173,22
PRIMER_LEFT_4_TM=60.308
PRIMER_RIGHT_4_TM=59.670
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_03070 Ps_contig_mira-and-tgicl-ass_franssen_28443
SEQUENCE=TCAACGCARNGTNYNCGGGTATAGCGGGTGACCACTCAAGAAACTTCCTTTGGTCCAATCCAAAACAAATAAAACTAAAAACAAAAAATCGATAAATAATAATAATAGATCTATAATCTCTCTATTATCATCTTCTTCTTTCTCATCACATACTCTCGTAAAACAATAATGGCTGGTTTCAGCGGCGATGAAACTGCTCCTTTCTTCGGCTTCCTCGGCGCCGCCGCTGCCCTAATTTTCTCATGTATGGGTGCGGCTTATGGAACTGCGAAGAGCGGTGTTGGTGTAGCGTCGATGGGTGTGATGAGACCCGAACTTGTTATGAAATCGATTGTGCCTGTTGTTATGGCTGGTGTCTTGGGTATTTATGGTTTGATTATCGCTGTTATTATCAGTACCGGCATTAACCCTAAGGCTAAATCTTATTATCTTTTTGATGGTTATGCTCATCTTTCTTCGGGGCTTGCTTGTGGTCTTGCTGGTTTGTCTGCTGGCATGGCTATTGGGATTGTTGGTGATGCTGGTGTTAGAGCAAATGCTCAACAGCCGAAGCTTTTTGTCGGTATGATTCTCATTCTCATCTTTGCTGAAGCATTGGCATTGTATGGTCTCATTGTTGGCATCATCCTCTCTTCCCGTGCTGGCCAATCCAGAGCTGAGTAATATCAATTTTATCATTGCTTGTAGGGCACATAGCCACAGATTCATGTATTGTGAACTTTATTAGAGACTTGGGCAGAGTAAGGGTGGAGCTTTGTCATGTTTTTCTTTCTTCTTATGTAGTCATTATAAAATTAACTCTCCTATGGAATAAAGCCTATCAAACTATTTCCCTGGCATTATTGTATTTATTGACGTTGAGTCAAACTAGATTAGTGATCTTTGTAAGAAACATTTTGTTTGTGTTGATTTCAATAATATTTAGTTGTGTTTACATTGAGACGTAGTATGATTATTTTGTATCAGTTCTGTACATCATGGATCATTTTCAGAAGTTGTCATTTATGAACAGAGCTTGGCTGTTGGTTCAGTAAATGCCGGATCGCGACAGTGCATTGGCTTGCTTGTGAAGTACTCAAACAACTT
TARGET=95,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03071 Ps_contig_mira-and-tgicl-ass_franssen_28448
SEQUENCE=TTTTGGCCTATTCTAATACTGGAAAACAAAACATCAAAGGTCTTTCCAACCTAACTGGCTATGTTAAGGGTAATGCTAATGGAGTTATCAACCTTGGTTCCTTGATAGCCTCTGCAAATGCTCAGAGACAAGCATGAATGAATGAATCCATGAAGCAATAATGTATATCGTGGGTGGAGGCAGCTACAATATAATTACTAATAAATAATATTATCTTATGTTCCCTT
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5726
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=0.351615
PRIMER_LEFT_SEQUENCE=TGGAGTTATCAACCTTGGTTCC
PRIMER_RIGHT_SEQUENCE=TATATTGTAGCTGCCTCCACCC
PRIMER_LEFT=79,22
PRIMER_RIGHT=192,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=60.352
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=0.6061
PRIMER_LEFT_1_PENALTY=0.254467
PRIMER_RIGHT_1_PENALTY=0.351615
PRIMER_LEFT_1_SEQUENCE=CTTGGTTCCTTGATAGCCTCTG
PRIMER_RIGHT_1_SEQUENCE=TATATTGTAGCTGCCTCCACCC
PRIMER_LEFT_1=92,22
PRIMER_RIGHT_1=192,22
PRIMER_LEFT_1_TM=60.254
PRIMER_RIGHT_1_TM=60.352
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.7117
PRIMER_LEFT_2_PENALTY=0.360050
PRIMER_RIGHT_2_PENALTY=0.351615
PRIMER_LEFT_2_SEQUENCE=AACCTTGGTTCCTTGATAGCCT
PRIMER_RIGHT_2_SEQUENCE=TATATTGTAGCTGCCTCCACCC
PRIMER_LEFT_2=89,22
PRIMER_RIGHT_2=192,22
PRIMER_LEFT_2_TM=60.360
PRIMER_RIGHT_2_TM=60.352
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=0.8344
PRIMER_LEFT_3_PENALTY=0.482784
PRIMER_RIGHT_3_PENALTY=0.351615
PRIMER_LEFT_3_SEQUENCE=TCAACCTTGGTTCCTTGATAGC
PRIMER_RIGHT_3_SEQUENCE=TATATTGTAGCTGCCTCCACCC
PRIMER_LEFT_3=87,22
PRIMER_RIGHT_3=192,22
PRIMER_LEFT_3_TM=60.483
PRIMER_RIGHT_3_TM=60.352
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.9808
PRIMER_LEFT_4_PENALTY=0.629225
PRIMER_RIGHT_4_PENALTY=0.351615
PRIMER_LEFT_4_SEQUENCE=AACATCAAAGGTCTTTCCAACC
PRIMER_RIGHT_4_SEQUENCE=TATATTGTAGCTGCCTCCACCC
PRIMER_LEFT_4=29,22
PRIMER_RIGHT_4=192,22
PRIMER_LEFT_4_TM=59.371
PRIMER_RIGHT_4_TM=60.352
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=164
=
PRIMER_SEQUENCE_ID=SSR_03072 Ps_contig_mira-and-tgicl-ass_franssen_28449
SEQUENCE=TTGCCTATTCTAATACTGGAAAACAAAACATCAAAGGTCTTTCCAACCTAACTGGCTATGTTAAGGGTAATGCTAATGGAGTTATCAACCTTGGTTCCTTGATAGCCTCTGCAAATGCTCAGAGACAAGCATGAATGAATGAATCCATGAAGCAATAATGTATATCTGGTGGAGCAGCTACAATATAATTACAATAAATAATNTTATCTTATGTTCCCTT
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3362
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=1.115242
PRIMER_LEFT_SEQUENCE=TGGAGTTATCAACCTTGGTTCC
PRIMER_RIGHT_SEQUENCE=ATATTGTAGCTGCTCCACCAGA
PRIMER_LEFT=76,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=58.885
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=1.4753
PRIMER_LEFT_1_PENALTY=0.360050
PRIMER_RIGHT_1_PENALTY=1.115242
PRIMER_LEFT_1_SEQUENCE=AACCTTGGTTCCTTGATAGCCT
PRIMER_RIGHT_1_SEQUENCE=ATATTGTAGCTGCTCCACCAGA
PRIMER_LEFT_1=86,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=60.360
PRIMER_RIGHT_1_TM=58.885
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=1.5980
PRIMER_LEFT_2_PENALTY=0.482784
PRIMER_RIGHT_2_PENALTY=1.115242
PRIMER_LEFT_2_SEQUENCE=TCAACCTTGGTTCCTTGATAGC
PRIMER_RIGHT_2_SEQUENCE=ATATTGTAGCTGCTCCACCAGA
PRIMER_LEFT_2=84,22
PRIMER_RIGHT_2=185,22
PRIMER_LEFT_2_TM=60.483
PRIMER_RIGHT_2_TM=58.885
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=102
PRIMER_PAIR_PENALTY_3=1.7445
PRIMER_LEFT_3_PENALTY=0.629225
PRIMER_RIGHT_3_PENALTY=1.115242
PRIMER_LEFT_3_SEQUENCE=AACATCAAAGGTCTTTCCAACC
PRIMER_RIGHT_3_SEQUENCE=ATATTGTAGCTGCTCCACCAGA
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=59.371
PRIMER_RIGHT_3_TM=58.885
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=1.9821
PRIMER_LEFT_4_PENALTY=0.866867
PRIMER_RIGHT_4_PENALTY=1.115242
PRIMER_LEFT_4_SEQUENCE=GGTCTTTCCAACCTAACTGGCT
PRIMER_RIGHT_4_SEQUENCE=ATATTGTAGCTGCTCCACCAGA
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=60.867
PRIMER_RIGHT_4_TM=58.885
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_03073 Ps_contig_mira-and-tgicl-ass_franssen_28450
SEQUENCE=AACGCAGAGTACNCGGGGAGAAACCTATCAAAAAAAACATTCATAACAAGTTTTTCAAGTTTTTCAATCAATGGCAGGTATTGCATCTGGAATCTGTAGCCTTGCTGTCGGCGGCGATGCACGGCGCCGACCAACACAGGCACAGCAGGGCTGTTCCAGCGATAACACTCCTGCTCCTCCTGCTCCTACGCCTTCGCCTGCACCTGCGCAGCAGTCATACGAAGAGTTTGCCTATTCTAATACTGGAAAACAAAACATCAAAGGTCTTTCCAACCTAACTGGCTATGTTAAGGGTAATGCTAATGGAGTTATCAACCTTGGTTCCTTGATAGCCTCTGCAAATGCTCAGAGACAAGCATGAATGAATGAATCCATGAAGCAATAATGTATATCTGGTGGAGGCAGCTCAATATAATTACTAACTAAATAAATATTATCTTATGTTGCCTCATCCTATTATAATGTGTGTCTTACACCATCATAGCCATTATACTACAATAAAATGTTGGTTAAATTTTAAATGTGTTAGTTAAAGTTTCATTTCATATATATATTCTACCAATACAAAATATTTACAATTGARCACC
TARGET=358,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03074 Ps_contig_mira-and-tgicl-ass_franssen_28450
SEQUENCE=AACGCAGAGTACNCGGGGAGAAACCTATCAAAAAAAACATTCATAACAAGTTTTTCAAGTTTTTCAATCAATGGCAGGTATTGCATCTGGAATCTGTAGCCTTGCTGTCGGCGGCGATGCACGGCGCCGACCAACACAGGCACAGCAGGGCTGTTCCAGCGATAACACTCCTGCTCCTCCTGCTCCTACGCCTTCGCCTGCACCTGCGCAGCAGTCATACGAAGAGTTTGCCTATTCTAATACTGGAAAACAAAACATCAAAGGTCTTTCCAACCTAACTGGCTATGTTAAGGGTAATGCTAATGGAGTTATCAACCTTGGTTCCTTGATAGCCTCTGCAAATGCTCAGAGACAAGCATGAATGAATGAATCCATGAAGCAATAATGTATATCTGGTGGAGGCAGCTCAATATAATTACTAACTAAATAAATATTATCTTATGTTGCCTCATCCTATTATAATGTGTGTCTTACACCATCATAGCCATTATACTACAATAAAATGTTGGTTAAATTTTAAATGTGTTAGTTAAAGTTTCATTTCATATATATATTCTACCAATACAAAATATTTACAATTGARCACC
TARGET=545,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03075 Ps_contig_mira-and-tgicl-ass_franssen_28452
SEQUENCE=CTTCAAATTACTGTAGTAATGTTTAGTATTATAACACCTGTATACAACAAAAAGTACAACCAAAGGGGGAAATACATAAACACTGATAAACAGAAAATCGACAACATTATTATTTATTTTATTTTATTGTTTGATGTTTAGAGGTTTGGATCAAACTACACAAACATGTCGGAAAGTGATGGAAGCTTGGTCTCACCCTTTTGACAAAGAGGAATGGTGAAATTGCCAATCACAGGAAGATCAATAATCAAACCAATATCCAATTGATAATCAATGTCCCAATCAGCACAAATATCCTTAGCCAAACTAATAATTATATTATGAGGCACTTTCACCAACACTTCAAGCAGTGTTGTGTCGCTTGCTTTCAATGATCCTGGATCTGGTATTATCCCTGATGCTATCTCCCTTGCAGCACTTTTGAAGGAGTAGTTGATCTCACAGATTGGAATTGAAGTTGAGTAAGGATTTGAAACATTAACCTTAGCCAAATACTCAACACTCTCGCGGCACACGCGCTTGAAATCAACGTCCGTTATACTCGCTGTTGGTTTCGGCATTTCACCGATTTTCTCAGTAACGTAGTTTTTGGCTTTGTCTAATAGCTGCGACATTTTCGACATTGTAAATATCAGACTCACACCCCYGATACC
TARGET=113,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03076 Ps_contig_mira-and-tgicl-ass_franssen_28453
SEQUENCE=ATTGACCTACAACACAATAATTCATTNCAAAATCACTATTTGTCACAACACAGAAAGATACACTTAAACCGAAAGTAAGCAAGACAGAGGACATTTACAATAAAAGTCTGAACTCTGTATGAAATTGAAAATGGTTCTTCATGTAAAGATCACATAAGCTTTTAAGTGTATTTTTTAACGTCTAAAAGTACGTGCGTAGACATAGATAAATACATACATATATGAAACCCTGAAGTGAACCTGAGCTATAGTTTAGTAGTTACAAAGTAGTTCAACTTATTTACAATGCAGAAACCAAATTATGAAAATATTCCTTAACATAATCATTCATTCATCCACAACTTCATAACTATGCAGCTTCAGCAAAACCAACTTGAAGATTTCCGTAGTCGAAAACAGTGTGATACGCCCTCATGAAAACATCGCCAAGAATCCAGAGTGGACCCTTAGGAGGTGGAATATCGAACGCAATAAATCCGCTCAGACAGACCTCTGTAATGCCTTCTCCGGTTCTTAGAATGTACTGTTCTGGTGTGAGGACGAAGGGTTTGTTTCCGATAGTGAATGAAATGTTTGGCATCTTAGAGATATCGCTACAGCTTATCACTGATTCTCCGGATGGACTTGGAAGACTCTCACACAGTTGATTCACGTAGTTGAACACTCTCTCCTTGTTCGCCTTTTGTTTTAGTTGATTCTGAATCCAAAGAACAAGCATCTGACAAGAAGAACACAGAGGAGTATCTTTTACCGACAACTCGCTATGTTCCTTTTCAGTCACCATCTCAATCCCAGCACTCATAGATTGATCCCGGCGGGTAGAACATAAACCAACTTGTGAACATACGTCACTAGGTTTTACCCCTGATACTAGGAGATCCCATATCAACTCTCCATATTGAGAAACAACTGTCTTACATTCTACACTCAGAACACCTTCAGCTCCAATGGCGTGGTTGATTTCAGCGACAACAGGAGTTGGACCAGCAAGCAAAGATGTTCCTGAGTCCACAATAGCAGCGCAGCCACCTTCACAAACACCTGTTGACGAGCCACCGATGAAAAAATCTCCCATTTCAATCTGCCAGTAACCTTTTTCAGTAACCGGAACGTAAGTGTGTTTTCCTTTGAAATGCTTAGGGTCGACACCACCAAAAATCAATTCACCACCCTTTTTCGCATTAGGATTCCCGTTGAGCCAAAAAGAGAACACCTTCTCACCGATAAGATTTTGTTCCACCATATTGTACCATACTGGCAAAGCTCTTGCAACTGAGATCTCCTGAAATCCAAGTCCAAATATTCCATCAAACTTTCCTGCCAGAAAAGAAAGACTTCCTTCTCGTGTAGCCTCAATGAAGTCCTGATGCTTGACAACAGAACTGCCAACTTTAACATGATCCTGACTGAAGAAACCAGATATTGATCCGGTTCCATAGCTTATTTTACATGATGTTCCGTTTTGGTTATATGTCTTGGATTTCTTTGCCTTGTACCAATTATGGGTATAGCATGCAAGAGAAAAGTAACATTTGGATGATGGAACCCAAAGGTTGGAACTACCGGTATCAAATATGACGGTAAATGTTTGTGGTGGCGTGCCAATGCCAATCTCACCGAAATACTGAGCATTCATATAATCTTTCAACGGTACTTCAGCTTCATCGTTTGACTTACCAATATACCGATCATGTGCACTCATCATTGGTCTTCCTGATCTTAGTTGATGTTCCCTTGCTTTTCTAAAAGCATTTATACTATGAATATCTAGAGGTCTCTTCTGTAAACCAATTCTCGTCATTCCGACGGAGAAAGAAGGAAGCAGAGAACATGTTAAAGCCCACAAACAAAACACCACAGCAAAATGCTTTTGCCCCATGTTGTCTTAATAGCTTGAGAAGCTGGTTTGGCTGCAGTTACAAAGGTTAGGTGAATGCCNNRAGTGTGTTAGAAGAAGC
TARGET=324,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03077 Ps_contig_mira-and-tgicl-ass_franssen_28455
SEQUENCE=TTGGTAGTTCTAGAAATCAACTAAAACAAAAAAGGACGAACAAGGAAGAGAAGTGTTCAACTAACGTGAATCAACTAGTGTGAGAGTNTTCCAAGTCCATCCGGAGAATCAGTGATAAGCTGTAGCGATATCTCTAAGATGCCAAACATTTCATTCACTATCGGAAACAAACCCTTCGTCCTCACACCAGAACAGTACATTCTAAGAACCGGAGTAAGGTCATTACAGAGGTCTGTCTGAGCGGGTTTATTGCGTTCGATATTCCTCCTCCCAAGGGTCCACTCTGGATTCTTGGCGATGTTTTCATGAGGGCGTATCACACTGTTTTCGACTACGGAAATCTTCAAGTTGGTTTTGCTGAAGCTGCATAGTGATGAAGTTGTGGATGAATGAATGATTATGTTAAGGAATATTTTCATAATTTCGTTTCTGCATTGTAAATAAGTTGAACTACTTTGTAACCTACTAAACTATAGCTCAGGTTCACTTCAAGGATTCATATATGTATGTATTTATCTATATCTATGCACGTACTTTTAGACGTGAAAAATACACTTAAAAGCTTATGTGATCTTACATGAAGAACCATTTTCATTTCATACAGAGTTCAGACTTTTATTAGTTAAATGTCCTTCTTTCTT
TARGET=386,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2871
PRIMER_LEFT_PENALTY=0.044097
PRIMER_RIGHT_PENALTY=0.243031
PRIMER_LEFT_SEQUENCE=TATTGCGTTCGATATTCCTCCT
PRIMER_RIGHT_SEQUENCE=AATCCTTGAAGTGAACCTGAGC
PRIMER_LEFT=247,22
PRIMER_RIGHT=496,22
PRIMER_LEFT_TM=59.956
PRIMER_RIGHT_TM=59.757
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=0.2891
PRIMER_LEFT_1_PENALTY=0.046061
PRIMER_RIGHT_1_PENALTY=0.243031
PRIMER_LEFT_1_SEQUENCE=TTTGCTGAAGCTGCATAGTGAT
PRIMER_RIGHT_1_SEQUENCE=AATCCTTGAAGTGAACCTGAGC
PRIMER_LEFT_1=353,22
PRIMER_RIGHT_1=496,22
PRIMER_LEFT_1_TM=60.046
PRIMER_RIGHT_1_TM=59.757
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=0.3246
PRIMER_LEFT_2_PENALTY=0.081536
PRIMER_RIGHT_2_PENALTY=0.243031
PRIMER_LEFT_2_SEQUENCE=GTTTTGCTGAAGCTGCATAGTG
PRIMER_RIGHT_2_SEQUENCE=AATCCTTGAAGTGAACCTGAGC
PRIMER_LEFT_2=351,22
PRIMER_RIGHT_2=496,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.3329
PRIMER_LEFT_3_PENALTY=0.044097
PRIMER_RIGHT_3_PENALTY=0.288804
PRIMER_LEFT_3_SEQUENCE=TATTGCGTTCGATATTCCTCCT
PRIMER_RIGHT_3_SEQUENCE=TGAATCCTTGAAGTGAACCTGA
PRIMER_LEFT_3=247,22
PRIMER_RIGHT_3=498,22
PRIMER_LEFT_3_TM=59.956
PRIMER_RIGHT_3_TM=59.711
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.3349
PRIMER_LEFT_4_PENALTY=0.046061
PRIMER_RIGHT_4_PENALTY=0.288804
PRIMER_LEFT_4_SEQUENCE=TTTGCTGAAGCTGCATAGTGAT
PRIMER_RIGHT_4_SEQUENCE=TGAATCCTTGAAGTGAACCTGA
PRIMER_LEFT_4=353,22
PRIMER_RIGHT_4=498,22
PRIMER_LEFT_4_TM=60.046
PRIMER_RIGHT_4_TM=59.711
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_03078 Ps_contig_mira-and-tgicl-ass_franssen_28459
SEQUENCE=AGAGAGAAAGGAAGAAGAATCATGTGGTGCTGGAAATAAAGCTAGAAAGAGAGAGAGGGAGAGAGTTTCTTCTACTTTGACAGCACTTTTAGGAGTTCTTGCAACTTTGGAAGTTCTTTGACAACTAACAAGCCTGAAGATAAATCAGAGATGCTGCTGCCTCCTTGCAGGCGACCGTTCTGGAAGAAACAGTAATGCTACTGAAATGGGAAAGAAAGGGAGTTGGTTTTCAGCAATCAAGAGGGTTTTCACACACCACTCCAAGGGGAAGGATTCGGATAACAAAAGTTTAAAAGAGAAGAAGAAAGGTCCGGGTAAACTAAAGCATGGAGAGACGAATTCTTTCATACCGCTCTTCAGGGAGCCGAGCAGCATTGAGAAAATATTTGGCGACTTCGAAAGAGAGCAACAAGTGCTTGCAATTAGGCCTCCCACGCCTCTTGAGCGACCCAAAACACCGCCTTTTGTTCCTCCTCGAGTTGCTTCTCCTAGGCCTCCTTCTCCAAAGCCCCCTTCTCCAAGAGCTTCATCTCCGAAGGCTCCTTCTCCACGAGTCACATCTCCCAAAGCTGCATCTTCCCGGAACGTTCATCAGAACAAGGAGGTTAGCTACAGACCTGAGCCGACTTTACGGAACCAACAAGCCTCGGCTACTAAGATTCAGGCTTTTTACAGAGGTTATACGGCGAGGAGGAGTTTTCGAGCTCTTAAGGGTTTGGTAAGGCTTCAAGGAGTGGTGAGAGGACAGAATGTGAAGAGACAGACAGTTAGTGCCATGAAACACATGCAGCTGTTAGTGCGAGTTCAATCTCAGATTCAATCTAGAAGAATCCAGATGCTGGAAAACCAGGCGCGATATCAAGCTGAGTTCAAGAACGATAAGGACGCTGCGAGTACCTTTGGAAAACTGACTTTAGGACATGGTTCTGAGGCAGGTAACAATGAAGATTGGGATGATAGTTTATTGACAAAAGAGGAAGTAGATGCAAGGTTGCAAAGGAAAGTGGAAGCAATCATCAAAAGAGAGAGAACAATGGCGTTTGCATATTCACATCAGTTGTGGAAGGCCACACCGAAATCAACTCAAACGCCGGTGACAGATATGCGATCGAGTGGATTCCCGTGGTGGTGGAACTGGTTGGAAAGACAACTACCTGCAACATCAAATCCACAAGAAAAACAAGTCCTAAAGAATTTCCAACTCACGCCGCCAAGACCATATTCAGAACAAAAGACAAGTCCGAGACCTGGCTCAAGCACTCAAAGGCAACAGCATCATCCTTTTGCCTTCGATAACATGGATACATCCACTCCAAAATCGACAAAATCGACTATCTTTCCATCTTCCAAACCATCTAGAACTCCTCCATTTAGGACTCCCCAAGGAAACAGTTCAAGCATGATGTCGAAATATTCAAGGTCAAGAGCAGTAGGGTCCAGTTCACCTTTCGACGTGCCAATGAAAGACGACGATAGTCTCACAAGCTGTCCGCCATTTTCGGTTCCAAACTACATGGCTTCAACTGCTTCTGCTAAGGCGAAAGTACGCGCTAGTAGTAATCCAAGAGAAAGATTTGGTGGTGGTGGTAGTAGCGGTTGCGTAACTCCAACTCCAAGCAGTGGTATCAACGCAGAGTCTCATTTCCTTTGTCACAAGGAATAGGATCATTCAAATGGAACAAAGGGTCATTGTTCTCTAACAATAAGGATCCTCATGCCTCTCACAGGACACCAGATAAATATCAGGAATCTATCGGAAACGTGAGCGTGGATTCGACGATATCATTACCGGCAAGGGTCGGAAGAAAGCCTTTTACAAGATTTGTGTGATTTAATTCTTTTTTAACCTATTTTTTTGTTTGTTTTTGTTTCATGTTTGGTGGGTGTTATTATCAGTTGTATTATGATTATGATCGAGTGTATTGGTGGTGATTATAGTTTTTGACAAAAGTATACTCTAATAAAAAAACATAATAGGAAATAATGTGGTTTGATAACTTTTGATTTGTAACTTTGTTTTTTTGAATCTAATGTGTTGATATAAAAGTTYATTTTTCTTTGTCACG
TARGET=48,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03079 Ps_contig_mira-and-tgicl-ass_franssen_28459
SEQUENCE=AGAGAGAAAGGAAGAAGAATCATGTGGTGCTGGAAATAAAGCTAGAAAGAGAGAGAGGGAGAGAGTTTCTTCTACTTTGACAGCACTTTTAGGAGTTCTTGCAACTTTGGAAGTTCTTTGACAACTAACAAGCCTGAAGATAAATCAGAGATGCTGCTGCCTCCTTGCAGGCGACCGTTCTGGAAGAAACAGTAATGCTACTGAAATGGGAAAGAAAGGGAGTTGGTTTTCAGCAATCAAGAGGGTTTTCACACACCACTCCAAGGGGAAGGATTCGGATAACAAAAGTTTAAAAGAGAAGAAGAAAGGTCCGGGTAAACTAAAGCATGGAGAGACGAATTCTTTCATACCGCTCTTCAGGGAGCCGAGCAGCATTGAGAAAATATTTGGCGACTTCGAAAGAGAGCAACAAGTGCTTGCAATTAGGCCTCCCACGCCTCTTGAGCGACCCAAAACACCGCCTTTTGTTCCTCCTCGAGTTGCTTCTCCTAGGCCTCCTTCTCCAAAGCCCCCTTCTCCAAGAGCTTCATCTCCGAAGGCTCCTTCTCCACGAGTCACATCTCCCAAAGCTGCATCTTCCCGGAACGTTCATCAGAACAAGGAGGTTAGCTACAGACCTGAGCCGACTTTACGGAACCAACAAGCCTCGGCTACTAAGATTCAGGCTTTTTACAGAGGTTATACGGCGAGGAGGAGTTTTCGAGCTCTTAAGGGTTTGGTAAGGCTTCAAGGAGTGGTGAGAGGACAGAATGTGAAGAGACAGACAGTTAGTGCCATGAAACACATGCAGCTGTTAGTGCGAGTTCAATCTCAGATTCAATCTAGAAGAATCCAGATGCTGGAAAACCAGGCGCGATATCAAGCTGAGTTCAAGAACGATAAGGACGCTGCGAGTACCTTTGGAAAACTGACTTTAGGACATGGTTCTGAGGCAGGTAACAATGAAGATTGGGATGATAGTTTATTGACAAAAGAGGAAGTAGATGCAAGGTTGCAAAGGAAAGTGGAAGCAATCATCAAAAGAGAGAGAACAATGGCGTTTGCATATTCACATCAGTTGTGGAAGGCCACACCGAAATCAACTCAAACGCCGGTGACAGATATGCGATCGAGTGGATTCCCGTGGTGGTGGAACTGGTTGGAAAGACAACTACCTGCAACATCAAATCCACAAGAAAAACAAGTCCTAAAGAATTTCCAACTCACGCCGCCAAGACCATATTCAGAACAAAAGACAAGTCCGAGACCTGGCTCAAGCACTCAAAGGCAACAGCATCATCCTTTTGCCTTCGATAACATGGATACATCCACTCCAAAATCGACAAAATCGACTATCTTTCCATCTTCCAAACCATCTAGAACTCCTCCATTTAGGACTCCCCAAGGAAACAGTTCAAGCATGATGTCGAAATATTCAAGGTCAAGAGCAGTAGGGTCCAGTTCACCTTTCGACGTGCCAATGAAAGACGACGATAGTCTCACAAGCTGTCCGCCATTTTCGGTTCCAAACTACATGGCTTCAACTGCTTCTGCTAAGGCGAAAGTACGCGCTAGTAGTAATCCAAGAGAAAGATTTGGTGGTGGTGGTAGTAGCGGTTGCGTAACTCCAACTCCAAGCAGTGGTATCAACGCAGAGTCTCATTTCCTTTGTCACAAGGAATAGGATCATTCAAATGGAACAAAGGGTCATTGTTCTCTAACAATAAGGATCCTCATGCCTCTCACAGGACACCAGATAAATATCAGGAATCTATCGGAAACGTGAGCGTGGATTCGACGATATCATTACCGGCAAGGGTCGGAAGAAAGCCTTTTACAAGATTTGTGTGATTTAATTCTTTTTTAACCTATTTTTTTGTTTGTTTTTGTTTCATGTTTGGTGGGTGTTATTATCAGTTGTATTATGATTATGATCGAGTGTATTGGTGGTGATTATAGTTTTTGACAAAAGTATACTCTAATAAAAAAACATAATAGGAAATAATGTGGTTTGATAACTTTTGATTTGTAACTTTGTTTTTTTGAATCTAATGTGTTGATATAAAAGTTYATTTTTCTTTGTCACG
TARGET=1576,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03080 Ps_contig_mira-and-tgicl-ass_franssen_28460
SEQUENCE=TTAAGGGACGTTCAAGGAGNGGTGAGAGGACAGAANAGTGAAGAGACAGACATAGTGCCATGAAACACATGCAGCTGTTAGTGCGAGTTCAATCTCAGATTCAATCTAGAAGAATCCAGATGCTGGAAAACCAGGCGCGATATCAAGCCGAGTTCAAGAACGATAAGGACGCTGCGAGTACCTTTGGAAAACTGACTTTAGGACATGGTTCTGAGGCAGGTAACAATGAAGATTGGGATGATAGTTTATTGACAAAAGAGGAAGTAGATGCAAGGTTGCAAAGGAAAGTGGAAGCAATCATCAAAAGAGAGAGAACAATGGCATTTGCATATTCACATCAGTTGTGGAAGGCCACACCAAAATCAACTCAAACACCGATGACAGATATGCGATCGAGTGGATTCCCGTGGTGGTGGAACTGGTTGGAAAGACAACTACCTGCAACATCAAATCCACAAGAAAAACAAGTCCTAAAGAATTTCCAACTCACGCCGCCAAGACCATATTCAGAACAAAAGACAAGTCCTAGACCTGGCTCAAGCACTCAAAGGCAACAGCATCATCCTTTTGCCTTCGATAACATGGATACATCCACTCCAAAATCGACAAAATCGACTATCTTTCCATCTTCCAAACCATCTAGAACTCCTCCATTTAGGACTCCCCAAGGAAACAGTTCAAGCATGATGTCAAAATATTCAAGGTCAAGAGCAGTAGGGTCCAGTTCACCTTTCGACGTGCCAATGAAAGACGACGATAGTCTCACAAGCTGTCCGCCATTTTCGGTTCCAAACTACATGGCTTCAACTGCTTCTGCTAAGGCGAAAGTACGCGCTAGTAGTAATCCAAGAGAAAGATTTGGTGGTGGTGGTAGTAGCGGTTGCGTAACTCCAACTCCAAGCAATGATTCAAAACGAAGAGTCTCATTTCCTTTGTCACAAGGAATAGGATCATTCAAATGGAACAAAGGGTCATTGTTCTCTAACAATAAGGATCCTCATGCCTCTCACAGGACACCAGAATAAATATCAGGAATCTATACGGAAACGTGAGCGTGGATTCGACGATATCACGTTACCGGRCGAAGGGGTACGGAAAGAAACGTCCTTTNACAAGTATTTGTGTGTATTTAATTTCTTTTTAACTNTTTTTTGTTGTTTTTGTTTCATTGGTTTTGGGTTGG
TARGET=860,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03081 Ps_contig_mira-and-tgicl-ass_franssen_28471
SEQUENCE=ATACTCTACTATAATTTTACTAAACCTAAACTTAGTTGGTACGAATACATTAACTAACAACTCAAAACCTAAACAGTTAACTAAAGAAAATAAAGAATTGTCATCATGAAATCAATGGTACATTATTTACAGTGAGAAAATAAAGTTACTTGAAGGGGAATTTTCCCCCTACCAACATCCAAGTACACATTCATTTGAATGAGGTGAGAACATAACTAGAACATCATGTACAGATAAAATACATAGAGAACATGACTTGCATACACAAGGCTAAGGGTCTGCCTTACCAGCCACCTGTTCATCACTAGAATCATATTCATCTTCAGTTAGCTCCTCATCTTCATCATCACTGGATTTCTCTTCGGTCGCAGCTCCAATGTCTTCTGGCTTGGCGTATTTCTCGCAGTACTCTTTAACCCTTTGGTCATAAGTAGGTCGGTCACGCATCATTAACGCCGCAGCTTCTCCATTCAACGGATCCGATGGATTTGGATACAAAAGGAGTTGTGGCAGAAACACCTCAAATACATTGACAAGATCAAACATGGGGCTCCAGGTCTGATTGATAACATCAAGACAAACCGATCCGGACATCTCATCAACATTTGGGTGATAGATCTTATTGATGAAGCCTATGGAAGGAGACTTATAAGGATAAGCATCTGGTAGCTCAACTCTTACTTTCCACACACCACCTTGATAAGGACTTTCATTTGGTCCATGAAAATGAACATAAAACTCTTGCATTCCATCGTTAATCATCTCCACTTTGTAATCACTCATCATCAGTTTCATCAAGTCCATCTCTCGTCGCTTGCTTGGGGAAGACATGTTTGGTTTCTGGTTTCTCTCTCTATGCTGGTTTGGTTTGGTTTGTTCCTTGCTTGCTCTATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGRAGAGAGNGNTGAGAGNGTNT
TARGET=860,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03082 Ps_contig_mira-and-tgicl-ass_franssen_28471
SEQUENCE=ATACTCTACTATAATTTTACTAAACCTAAACTTAGTTGGTACGAATACATTAACTAACAACTCAAAACCTAAACAGTTAACTAAAGAAAATAAAGAATTGTCATCATGAAATCAATGGTACATTATTTACAGTGAGAAAATAAAGTTACTTGAAGGGGAATTTTCCCCCTACCAACATCCAAGTACACATTCATTTGAATGAGGTGAGAACATAACTAGAACATCATGTACAGATAAAATACATAGAGAACATGACTTGCATACACAAGGCTAAGGGTCTGCCTTACCAGCCACCTGTTCATCACTAGAATCATATTCATCTTCAGTTAGCTCCTCATCTTCATCATCACTGGATTTCTCTTCGGTCGCAGCTCCAATGTCTTCTGGCTTGGCGTATTTCTCGCAGTACTCTTTAACCCTTTGGTCATAAGTAGGTCGGTCACGCATCATTAACGCCGCAGCTTCTCCATTCAACGGATCCGATGGATTTGGATACAAAAGGAGTTGTGGCAGAAACACCTCAAATACATTGACAAGATCAAACATGGGGCTCCAGGTCTGATTGATAACATCAAGACAAACCGATCCGGACATCTCATCAACATTTGGGTGATAGATCTTATTGATGAAGCCTATGGAAGGAGACTTATAAGGATAAGCATCTGGTAGCTCAACTCTTACTTTCCACACACCACCTTGATAAGGACTTTCATTTGGTCCATGAAAATGAACATAAAACTCTTGCATTCCATCGTTAATCATCTCCACTTTGTAATCACTCATCATCAGTTTCATCAAGTCCATCTCTCGTCGCTTGCTTGGGGAAGACATGTTTGGTTTCTGGTTTCTCTCTCTATGCTGGTTTGGTTTGGTTTGTTCCTTGCTTGCTCTATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGRAGAGAGNGNTGAGAGNGTNT
TARGET=894,44
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03083 Ps_contig_mira-and-tgicl-ass_franssen_28478
SEQUENCE=ACACAACACGTTAATTTTATCTTCACTCTTCTTCAGATCTCAAATTTCATTTCTCGCTCACATCGATTTCTCCTCCTCCTCCATGGCCGCTATTACTTCCATCAAGGCTCGTCAGATCTTCGACAGCCGTGGCAATCCAACCGTGAGGTTGATATAACTGTCTCCGATGGCACTTTCGCTAGAGCCGCT
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5104
PRIMER_LEFT_PENALTY=1.484846
PRIMER_RIGHT_PENALTY=0.025566
PRIMER_LEFT_SEQUENCE=ATTTCATTTCTCGCTCACATCG
PRIMER_RIGHT_SEQUENCE=AAGTGCCATCGGAGACAGTTAT
PRIMER_LEFT=43,22
PRIMER_RIGHT=174,22
PRIMER_LEFT_TM=61.485
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.6000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=2.0775
PRIMER_LEFT_1_PENALTY=2.051981
PRIMER_RIGHT_1_PENALTY=0.025566
PRIMER_LEFT_1_SEQUENCE=TTCATTTCTCGCTCACATCG
PRIMER_RIGHT_1_SEQUENCE=AAGTGCCATCGGAGACAGTTAT
PRIMER_LEFT_1=45,20
PRIMER_RIGHT_1=174,22
PRIMER_LEFT_1_TM=59.948
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=2.3140
PRIMER_LEFT_2_PENALTY=2.288441
PRIMER_RIGHT_2_PENALTY=0.025566
PRIMER_LEFT_2_SEQUENCE=TTTCATTTCTCGCTCACATCG
PRIMER_RIGHT_2_SEQUENCE=AAGTGCCATCGGAGACAGTTAT
PRIMER_LEFT_2=44,21
PRIMER_RIGHT_2=174,22
PRIMER_LEFT_2_TM=61.288
PRIMER_RIGHT_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=131
PRIMER_PAIR_PENALTY_3=2.5181
PRIMER_LEFT_3_PENALTY=1.484846
PRIMER_RIGHT_3_PENALTY=1.033298
PRIMER_LEFT_3_SEQUENCE=ATTTCATTTCTCGCTCACATCG
PRIMER_RIGHT_3_SEQUENCE=AAAGTGCCATCGGAGACAGTTA
PRIMER_LEFT_3=43,22
PRIMER_RIGHT_3=175,22
PRIMER_LEFT_3_TM=61.485
PRIMER_RIGHT_3_TM=61.033
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=2.7360
PRIMER_LEFT_4_PENALTY=1.484846
PRIMER_RIGHT_4_PENALTY=1.251196
PRIMER_LEFT_4_SEQUENCE=ATTTCATTTCTCGCTCACATCG
PRIMER_RIGHT_4_SEQUENCE=AAGTGCCATCGGAGACAGTTA
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=174,21
PRIMER_LEFT_4_TM=61.485
PRIMER_RIGHT_4_TM=59.749
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.6000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_03084 Ps_contig_mira-and-tgicl-ass_franssen_28487
SEQUENCE=ATTCACAAGTATTCAATTTTTTNGTAGAAGTCAAGAATAATAATAATAATAATTGGAACACAAAAACTACCATCACCACTAAGATTCCTCACATATCTATTCAGACCTCTTAGCATTGCCAACACCGAAATCACATACTAGAATGGCAACAACAACCTTTGCAACCGTCGTAGGAATGGGTGGTGCATCACGTCTCACCTCAGGATTTGGGAAACCACAAGTAACTGCTAGGAATCCTTTGAGGCAAGCTATGGCGGTAGGAAATGGTGGAAGAGTGACATGCTTCCAGAGGGATTGGCTACGGAGGGATTTCAATGTGATTGGGTTTGGGTTGATAGGTTGGTTGGCACCGTCTAGCATCCCGGCTATTGACGGTAAAAGTCTGACTGGACTTTTCTTTGATAGTATTGGAACTGAACTTGCTCACTTCCCTACACCTCCAGCTCTTACTTCACAGTTTTGGTTATGGTTGATCTGTTGGCACTTGGGGCTGTTCATCTCCCTCACTTTCGGTCAAATTGGTTTCAAGGGAAGGACTGAGGAGTACTTTGATTCATAATAACTTATGGTCTGTTATTAATTGCTTTTCCTTTTATATTATTTTCTGCCTTGTAATCTACCATCTGTAACTCTGGTACCTTTTCCATTGCACTAGAAAAAATCGGTGTAATATCGCTTTCATAATGAATCAATGTTATATTAGTATTATGTTAGTAGTNTTNTGTGTATTAGTTAGTAA
TARGET=36,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03085 Ps_contig_mira-and-tgicl-ass_franssen_28492
SEQUENCE=CAACTCAGAGAGGCAGTAGGTTCTGGAGGATGCTGGTGGTGCTGCTGACAGGGTTTTGAATCAACTGCTTACAGAAATGGATGGAATGTCGGCAAAGAAAACTGTGTTTATCATTGGGGCCACTAATCGACCAGACATTATAGACCCTGCTCTACTGCGACCTGGACGTCTGGACCAGTTGATTTATATCCCCCTTCCAGATGAGGATTCACGCCATCAGATATTCAAAGCTGCCTTGAGAAAATCCCCCATTGCAAAGGATGTTGACATTAGAGCTCTTGCCAAGTACACTCAAGGCTTCAGTGGTGCTGATATTACTGAAATTTGCCAGCGTGCATGCAAGTATGCTATAAGAGAGAACATTGAGAAGGATATTGAAAGAGAAAGGAGGAGAAGTGATAACCCTGAGGCTATGGAAGAAGATAATGAAGATGATGAGGTGGCTGAAATCAAAGCAGCACATTTTGAAGAGTCAATGAAGTACGCACGCAGGAGTGTTAGTGATGCTGATATCCGCAAGTACCAAGCATTTGCCCAAACCTTGCAGCAATCAAGAGGGTTTGGAAGCGAGTTCAGGTTTGCTGACACCAGTGCTGGTGGTACGGCCACTGCTGCTGCTGATCCTTTTGCTAGTGCTGGCGGAGCTGATGAAGATGACCTTTACAGTTAGTTCAACATTTGCTTATTTTTATTCTGGAACTACAATGCTGCCTAATTTATGTACTCTTTTATTTGTTTGTATGAAATCTGGCTTCCATTAGATTATTAATATCATTGCTCCGTAAAAGATATTATTGGTACATCATTTAGGTGCGAGCCTCTCTTGCTTTGAAACAGTTATTTGGTCAGTTTCTAGGATCATTTATGGTCTTGGGTTCTCAGCTCTGGTTATTTCAATTGTATTTCTGAGCTTACACATTTTTAACAGCAATGCTTTTAAGTAAACTTAAAGGAATAAATTTAAGAATCTACATTTAGTNAAC
TARGET=609,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1273
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.095538
PRIMER_LEFT_SEQUENCE=AAGATGATGAGGTGGCTGAAAT
PRIMER_RIGHT_SEQUENCE=TAGGCAGCATTGTAGTTCCAGA
PRIMER_LEFT=428,22
PRIMER_RIGHT=713,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.1491
PRIMER_LEFT_1_PENALTY=0.053515
PRIMER_RIGHT_1_PENALTY=0.095538
PRIMER_LEFT_1_SEQUENCE=CAAAGCAGCACATTTTGAAGAG
PRIMER_RIGHT_1_SEQUENCE=TAGGCAGCATTGTAGTTCCAGA
PRIMER_LEFT_1=450,22
PRIMER_RIGHT_1=713,22
PRIMER_LEFT_1_TM=60.054
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.1549
PRIMER_LEFT_2_PENALTY=0.059316
PRIMER_RIGHT_2_PENALTY=0.095538
PRIMER_LEFT_2_SEQUENCE=AGCAGCACATTTTGAAGAGTCA
PRIMER_RIGHT_2_SEQUENCE=TAGGCAGCATTGTAGTTCCAGA
PRIMER_LEFT_2=453,22
PRIMER_RIGHT_2=713,22
PRIMER_LEFT_2_TM=60.059
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.3350
PRIMER_LEFT_3_PENALTY=0.053515
PRIMER_RIGHT_3_PENALTY=0.281487
PRIMER_LEFT_3_SEQUENCE=CAAAGCAGCACATTTTGAAGAG
PRIMER_RIGHT_3_SEQUENCE=AAAGGTCATCTTCATCAGCTCC
PRIMER_LEFT_3=450,22
PRIMER_RIGHT_3=661,22
PRIMER_LEFT_3_TM=60.054
PRIMER_RIGHT_3_TM=59.719
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.3408
PRIMER_LEFT_4_PENALTY=0.059316
PRIMER_RIGHT_4_PENALTY=0.281487
PRIMER_LEFT_4_SEQUENCE=AGCAGCACATTTTGAAGAGTCA
PRIMER_RIGHT_4_SEQUENCE=AAAGGTCATCTTCATCAGCTCC
PRIMER_LEFT_4=453,22
PRIMER_RIGHT_4=661,22
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=59.719
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_03086 Ps_contig_mira-and-tgicl-ass_franssen_28497
SEQUENCE=AAAGCAGTGGTATCAACGCAGAGTCAATTGTGATTGGAAAGGGAACTTTCTGTAGAATGTGTATATTTATGAGTGTTCATGGTGCGATTGAGTCCTAAAGGGGGTTATGCATTTGTGGCTAGGAGCTTCGCGTGTCATTCCTCTGTTTTGTTCTTACCATATTGCGTGAGAATATAGGGTTGTCTGTGGGGCAATTCGTGCAGTTTTGGCCTATTGTATTGTTATTTATTTATCCTACAAGATTCTGAAATGTATCATATGAAGTATCGGTCCTATTTATGCATTGAGGTTGCAGACATTTGTTTATCCAANCNANAGTTTGCCACAATTTAGAAGTTATTCCGTCAGCATTTATTAGAAA
TARGET=222,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1483
PRIMER_LEFT_PENALTY=0.110942
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=ATTTATGAGTGTTCATGGTGCG
PRIMER_RIGHT_SEQUENCE=ACAAATGTCTGCAACCTCAATG
PRIMER_LEFT=64,22
PRIMER_RIGHT=302,22
PRIMER_LEFT_TM=59.889
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.2345
PRIMER_LEFT_1_PENALTY=0.197140
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=AGTGGTATCAACGCAGAGTCAA
PRIMER_RIGHT_1_SEQUENCE=ACAAATGTCTGCAACCTCAATG
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=302,22
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.2583
PRIMER_LEFT_2_PENALTY=0.220992
PRIMER_RIGHT_2_PENALTY=0.037339
PRIMER_LEFT_2_SEQUENCE=AGTCAATTGTGATTGGAAAGGG
PRIMER_RIGHT_2_SEQUENCE=ACAAATGTCTGCAACCTCAATG
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=302,22
PRIMER_LEFT_2_TM=60.221
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.4321
PRIMER_LEFT_3_PENALTY=0.394711
PRIMER_RIGHT_3_PENALTY=0.037339
PRIMER_LEFT_3_SEQUENCE=TATGAGTGTTCATGGTGCGATT
PRIMER_RIGHT_3_SEQUENCE=ACAAATGTCTGCAACCTCAATG
PRIMER_LEFT_3=67,22
PRIMER_RIGHT_3=302,22
PRIMER_LEFT_3_TM=60.395
PRIMER_RIGHT_3_TM=60.037
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.4321
PRIMER_LEFT_4_PENALTY=0.394711
PRIMER_RIGHT_4_PENALTY=0.037339
PRIMER_LEFT_4_SEQUENCE=TTATGAGTGTTCATGGTGCGAT
PRIMER_RIGHT_4_SEQUENCE=ACAAATGTCTGCAACCTCAATG
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=302,22
PRIMER_LEFT_4_TM=60.395
PRIMER_RIGHT_4_TM=60.037
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.8000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_03087 Ps_contig_mira-and-tgicl-ass_franssen_28498
SEQUENCE=AATTCGAAAAGAAACAATTTAATTTTTCTAACTGCCCTTAAAAAACTAGAATCCCTCGATCCCAATCAAAATACACCAACTTACCCCACTACAGAATTAACAGCTGATCCCATTCTATCTAGACTAATGAAGTCATAAATTCAATAATGCTGACGGAATACTTCTAATTGTGGACAAACTGTAGATTGGATAAACAAATGTCTGCAACCTCAATGCATAAATAGGACCGATACTTCATATGATACATTTCAGAATCTTGTAGGATAAATAAATAACAATACAATAGGCCAAAACTGCACGAATTGCCCCACAGACAACCCTATATTCTCACGCAATATGGTAAAGAACAAAACAGAGGAATGACACGCGAAGCTCCTAGCCAACAAATGCCAACTTAACCCCCCTTTAGGACTCAATCCGCACCATGAACACTCAATAAATATACACTATCTAACAGAAAGTTTCCCTTTCCAATTCCAAC
TARGET=264,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4396
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.402305
PRIMER_LEFT_SEQUENCE=ACAAATGTCTGCAACCTCAATG
PRIMER_RIGHT_SEQUENCE=GTTAAGTTGGCATTTGTTGGCT
PRIMER_LEFT=193,22
PRIMER_RIGHT=398,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.402
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.4442
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.406861
PRIMER_LEFT_1_SEQUENCE=ACAAATGTCTGCAACCTCAATG
PRIMER_RIGHT_1_SEQUENCE=GAATATAGGGTTGTCTGTGGGG
PRIMER_LEFT_1=193,22
PRIMER_RIGHT_1=326,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.593
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=0.5765
PRIMER_LEFT_2_PENALTY=0.169677
PRIMER_RIGHT_2_PENALTY=0.406861
PRIMER_LEFT_2_SEQUENCE=ACTAGAATCCCTCGATCCCAAT
PRIMER_RIGHT_2_SEQUENCE=GAATATAGGGTTGTCTGTGGGG
PRIMER_LEFT_2=44,22
PRIMER_RIGHT_2=326,22
PRIMER_LEFT_2_TM=60.170
PRIMER_RIGHT_2_TM=59.593
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.5890
PRIMER_LEFT_3_PENALTY=0.037339
PRIMER_RIGHT_3_PENALTY=0.551677
PRIMER_LEFT_3_SEQUENCE=ACAAATGTCTGCAACCTCAATG
PRIMER_RIGHT_3_SEQUENCE=GTTGGAATTGGAAAGGGAAACT
PRIMER_LEFT_3=193,22
PRIMER_RIGHT_3=480,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=60.552
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.5952
PRIMER_LEFT_4_PENALTY=0.188292
PRIMER_RIGHT_4_PENALTY=0.406861
PRIMER_LEFT_4_SEQUENCE=AAAACTAGAATCCCTCGATCCC
PRIMER_RIGHT_4_SEQUENCE=GAATATAGGGTTGTCTGTGGGG
PRIMER_LEFT_4=41,22
PRIMER_RIGHT_4=326,22
PRIMER_LEFT_4_TM=59.812
PRIMER_RIGHT_4_TM=59.593
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.3000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_03088 Ps_contig_mira-and-tgicl-ass_franssen_28524
SEQUENCE=TTTAATTAAGTAAAAACGTAAAATAAGTACGTCCAACGAAGGAAGGAAGTCAAGAAGTACACATATTATGATATTACACACAACACAGAGTTCATTCTGAATACATTATACAGATNANTAACTAAYNRTNAGTAGAAATACTAAAATACCCTCGGTTGGGCCTGAGAATCAGATTCATTCTCAGCCTCGCTTGACAGGGTCGAAATTGGAAACACCGATAACAGCACCAAAGAATGGCCGTAACCACAACTCCACCAGAAACAATACTGAGCAAGAAATTCATGGCGGGTGATGTTAAAGCTTCGTTTGGTGAGTCATGGAGAAAGGTTCTCTGTGAAAGTGAAGTTGTGGAAGGAAAAATCTCTGCTGGTAGTCCTTCTGTTCTTGTTGTCTCTTACTCTG
TARGET=38,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03089 Ps_contig_mira-and-tgicl-ass_franssen_28536
SEQUENCE=GGTAAAGGGGTAAAGGTTAAGTTAACTAAGGGAAGTAAGGTTAGTATAGTCAGGAAGTTGCAGGGTAGTGATGAATGTAGTGTCCCCACTCGTATAAACTGCTGCTGCTGCTGCTGCTGCTGTTGTTGAAGCTGTTGTTGAAGCTGCTGCTGTTGAGGCTGCTGTTGCTGTTGTTGATGTTGAGGCTGCTGTTGCTGTTGATGTTGAGGCTGCTGTTGTTGTTGTTGAGGCTGCTGTTGCTGCTGCTGGAGTTCAAACTGTTGCTGATGTTGCTGATGTTGCTGTAGTTCGAATTGCTGCTGTTGCTGCTGGAGTTCAAATTGTTGTTGCAGTAAATATCCAGGGTCCTGTACGTGTTGCTGCAATTGGATCCTACCCTGTGGATCAGATAAATTGAGTTTCGGATCCACAGGGTTACTTAGAACCCTTGTAGCTCCAGATTGAATCTGAACTTGCTCTTGATAGATAACAGGTTTTTGACGTGACATTGGATTTGAAAGACTACTATTATCACTTGCAACTGAATCAGGAGAAGGCAAATCAGCGCCTCCTCCACCACTCGACTTTTGTTGAAATTGAGGAGGTTGTTGTGGTTGTTGCTGAGTTTGAGCCGGTGGCTGTTGAACAACCTGTGGCTGAGGATTTGGTGGTTTTCTATATCCCACAGCCACTCCATGATCAGATCTCTCATCATCAGAGAAAACTCTATTCTGATATTCACCAACAGCCACAGCAGAACCAATCGGAACACCAATCACCGCCGTAGCAAGAGTAGTCGGAACCGGTGGATGAGGCGGCGAAGACATCACAGCAAATCCCTCATCCTGCTTCTGAACAACACCAACTCCCATTTGAGCAAACTGTTCTTCAATCCCCAAAACCTTCTGATCCTGCTGTTGCAGCTGTTGCTGCTGAACCCTAACTCCAGCAGAATTACCATCTTCCACATGCACCCTAATTGGAGGCAAATTCGCCAACGACGGAGACGAGGAGGTTGAACCAAAAGACGACGAAGTTTCCATCATAGGAGAATCAGGAACCGAGTGAACATCCTGCTTCAAATTCTTACCATTCCCAAAAGATCCAGGTTGAGAAACACCTTCAGGAGGTTCTCTAGAGCCAGGTTCAATATTATTCCCAGCTACATCATCATCAAGACCTAAAAGATTGTTAACAGAAGCCGATTCAGAAAACCCTCGATTAAGCAATCCAGCACCGTTTAACGCATCAAGGAACCAATCATCCGATTTAGCCGAAGTATCGAGAATTTGTGGCGGAATCGAGTGAGCAGATTCAGGTTTAGTCGGAAAAAGAAAAAGCCGAATTCGCGAGGGTTTGATAGAAGAATTCGCCGAAGCCGTTCGATCGTATTCATCGATCATGTTTTCCAAATCTTCATCAGTGGTAACAGAAATCAATGAATCTAAATCTTCGTTCGGAAGCTGATACTTCAGCGTGAACGGTCTTCCGTTAAGGAAGGTTTTCGAGAGGCGCGTGGATAACTCCGCGAGTGAGGTTACACGCTCAACCACCACGATTCGAGTATCACCGCCGACGTAACAGAGAGATTTATCATGTGGACGAGGAACGATGTGGCCGCCGTAACTGCACATGAGACGGAGTTTCGTTGACGCCGGAGGGAAAGGTTCGTCCCACGAATCCGTGTTGCGAGAACGCGGAGAGGAGTCGACTGAATCGGGGTAGTTGAGGTGTGGTGGCGGTGGTGCAACGGAGGATAACGGCGGTGCCGTTGACGGCGTTGGAGGTTGGGGTTGCGCCTCCATGATCAAGAAGAAGAATAATAACTGAATAAGGTTTTTGGTGCTGCTGCAGCAGCCCCTTTTTGCAGCGCAAAATAAAAAGAAAGGAAAAAAGAG
TARGET=99,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6008
PRIMER_LEFT_PENALTY=0.548390
PRIMER_RIGHT_PENALTY=0.052366
PRIMER_LEFT_SEQUENCE=AGGAAGTTGCAGGGTAGTGATG
PRIMER_RIGHT_SEQUENCE=CAGCAATTCGAACTACAGCAAC
PRIMER_LEFT=51,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=60.548
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.6008
PRIMER_LEFT_1_PENALTY=0.548390
PRIMER_RIGHT_1_PENALTY=0.052366
PRIMER_LEFT_1_SEQUENCE=AGGAAGTTGCAGGGTAGTGATG
PRIMER_RIGHT_1_SEQUENCE=AGCAATTCGAACTACAGCAACA
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=297,22
PRIMER_LEFT_1_TM=60.548
PRIMER_RIGHT_1_TM=59.948
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.6008
PRIMER_LEFT_2_PENALTY=0.548390
PRIMER_RIGHT_2_PENALTY=0.052366
PRIMER_LEFT_2_SEQUENCE=CAGGAAGTTGCAGGGTAGTGAT
PRIMER_RIGHT_2_SEQUENCE=AGCAATTCGAACTACAGCAACA
PRIMER_LEFT_2=50,22
PRIMER_RIGHT_2=297,22
PRIMER_LEFT_2_TM=60.548
PRIMER_RIGHT_2_TM=59.948
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=0.6008
PRIMER_LEFT_3_PENALTY=0.548390
PRIMER_RIGHT_3_PENALTY=0.052366
PRIMER_LEFT_3_SEQUENCE=CAGGAAGTTGCAGGGTAGTGAT
PRIMER_RIGHT_3_SEQUENCE=CAGCAATTCGAACTACAGCAAC
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=298,22
PRIMER_LEFT_3_TM=60.548
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.6919
PRIMER_LEFT_4_PENALTY=0.548390
PRIMER_RIGHT_4_PENALTY=0.143555
PRIMER_LEFT_4_SEQUENCE=AGGAAGTTGCAGGGTAGTGATG
PRIMER_RIGHT_4_SEQUENCE=CAGGACCCTGGATATTTACTGC
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=349,22
PRIMER_LEFT_4_TM=60.548
PRIMER_RIGHT_4_TM=59.856
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_03090 Ps_contig_mira-and-tgicl-ass_franssen_28536
SEQUENCE=GGTAAAGGGGTAAAGGTTAAGTTAACTAAGGGAAGTAAGGTTAGTATAGTCAGGAAGTTGCAGGGTAGTGATGAATGTAGTGTCCCCACTCGTATAAACTGCTGCTGCTGCTGCTGCTGCTGTTGTTGAAGCTGTTGTTGAAGCTGCTGCTGTTGAGGCTGCTGTTGCTGTTGTTGATGTTGAGGCTGCTGTTGCTGTTGATGTTGAGGCTGCTGTTGTTGTTGTTGAGGCTGCTGTTGCTGCTGCTGGAGTTCAAACTGTTGCTGATGTTGCTGATGTTGCTGTAGTTCGAATTGCTGCTGTTGCTGCTGGAGTTCAAATTGTTGTTGCAGTAAATATCCAGGGTCCTGTACGTGTTGCTGCAATTGGATCCTACCCTGTGGATCAGATAAATTGAGTTTCGGATCCACAGGGTTACTTAGAACCCTTGTAGCTCCAGATTGAATCTGAACTTGCTCTTGATAGATAACAGGTTTTTGACGTGACATTGGATTTGAAAGACTACTATTATCACTTGCAACTGAATCAGGAGAAGGCAAATCAGCGCCTCCTCCACCACTCGACTTTTGTTGAAATTGAGGAGGTTGTTGTGGTTGTTGCTGAGTTTGAGCCGGTGGCTGTTGAACAACCTGTGGCTGAGGATTTGGTGGTTTTCTATATCCCACAGCCACTCCATGATCAGATCTCTCATCATCAGAGAAAACTCTATTCTGATATTCACCAACAGCCACAGCAGAACCAATCGGAACACCAATCACCGCCGTAGCAAGAGTAGTCGGAACCGGTGGATGAGGCGGCGAAGACATCACAGCAAATCCCTCATCCTGCTTCTGAACAACACCAACTCCCATTTGAGCAAACTGTTCTTCAATCCCCAAAACCTTCTGATCCTGCTGTTGCAGCTGTTGCTGCTGAACCCTAACTCCAGCAGAATTACCATCTTCCACATGCACCCTAATTGGAGGCAAATTCGCCAACGACGGAGACGAGGAGGTTGAACCAAAAGACGACGAAGTTTCCATCATAGGAGAATCAGGAACCGAGTGAACATCCTGCTTCAAATTCTTACCATTCCCAAAAGATCCAGGTTGAGAAACACCTTCAGGAGGTTCTCTAGAGCCAGGTTCAATATTATTCCCAGCTACATCATCATCAAGACCTAAAAGATTGTTAACAGAAGCCGATTCAGAAAACCCTCGATTAAGCAATCCAGCACCGTTTAACGCATCAAGGAACCAATCATCCGATTTAGCCGAAGTATCGAGAATTTGTGGCGGAATCGAGTGAGCAGATTCAGGTTTAGTCGGAAAAAGAAAAAGCCGAATTCGCGAGGGTTTGATAGAAGAATTCGCCGAAGCCGTTCGATCGTATTCATCGATCATGTTTTCCAAATCTTCATCAGTGGTAACAGAAATCAATGAATCTAAATCTTCGTTCGGAAGCTGATACTTCAGCGTGAACGGTCTTCCGTTAAGGAAGGTTTTCGAGAGGCGCGTGGATAACTCCGCGAGTGAGGTTACACGCTCAACCACCACGATTCGAGTATCACCGCCGACGTAACAGAGAGATTTATCATGTGGACGAGGAACGATGTGGCCGCCGTAACTGCACATGAGACGGAGTTTCGTTGACGCCGGAGGGAAAGGTTCGTCCCACGAATCCGTGTTGCGAGAACGCGGAGAGGAGTCGACTGAATCGGGGTAGTTGAGGTGTGGTGGCGGTGGTGCAACGGAGGATAACGGCGGTGCCGTTGACGGCGTTGGAGGTTGGGGTTGCGCCTCCATGATCAAGAAGAAGAATAATAACTGAATAAGGTTTTTGGTGCTGCTGCAGCAGCCCCTTTTTGCAGCGCAAAATAAAAAGAAAGGAAAAAAGAG
TARGET=214,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1678
PRIMER_LEFT_PENALTY=0.037486
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=CCACTCGTATAAACTGCTGCTG
PRIMER_RIGHT_SEQUENCE=CACGTACAGGACCCTGGATATT
PRIMER_LEFT=85,22
PRIMER_RIGHT=355,22
PRIMER_LEFT_TM=59.963
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.1810
PRIMER_LEFT_1_PENALTY=0.037486
PRIMER_RIGHT_1_PENALTY=0.143555
PRIMER_LEFT_1_SEQUENCE=CCACTCGTATAAACTGCTGCTG
PRIMER_RIGHT_1_SEQUENCE=CAGGACCCTGGATATTTACTGC
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=349,22
PRIMER_LEFT_1_TM=59.963
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.2260
PRIMER_LEFT_2_PENALTY=0.095684
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=GTTGAAGCTGTTGTTGAAGCTG
PRIMER_RIGHT_2_SEQUENCE=CACGTACAGGACCCTGGATATT
PRIMER_LEFT_2=124,22
PRIMER_RIGHT_2=355,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.2392
PRIMER_LEFT_3_PENALTY=0.095684
PRIMER_RIGHT_3_PENALTY=0.143555
PRIMER_LEFT_3_SEQUENCE=GTTGAAGCTGTTGTTGAAGCTG
PRIMER_RIGHT_3_SEQUENCE=CAGGACCCTGGATATTTACTGC
PRIMER_LEFT_3=124,22
PRIMER_RIGHT_3=349,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.2415
PRIMER_LEFT_4_PENALTY=0.037486
PRIMER_RIGHT_4_PENALTY=0.203971
PRIMER_LEFT_4_SEQUENCE=CCACTCGTATAAACTGCTGCTG
PRIMER_RIGHT_4_SEQUENCE=GGACCCTGGATATTTACTGCAA
PRIMER_LEFT_4=85,22
PRIMER_RIGHT_4=347,22
PRIMER_LEFT_4_TM=59.963
PRIMER_RIGHT_4_TM=60.204
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_03091 Ps_contig_mira-and-tgicl-ass_franssen_28537
SEQUENCE=TAACACACAGCCATTCAGAANGTNGNANANACARACAACAAGNAGCCTCAACATTCAACAAGCAACAGCAGCCTCAACATCAACAACAGCAACAGCAGCCTCAACAGCAGCAGCTTCAACAACAGCTTCAACAACAGCAGCAGCAGCAGCAGCAGCAGTTTATACATGGGGGACACTACATTCATCATAACCCTGCAATTCCTGCATACTATCCTGTATATCCTTCACAGCAACAGCCCCATCATCAGGTTTACTATGTTCCTGCAAGACAGCCACAAGCTTACAATCTTCCTGTGCAGCAGGCTAATATAGGGGATTCTGCCACAACAATGTCTTCTAGTAGACCCCAAAACCCTCCAAACCCTACTACACTGGTTCAACCAAATGCAGCTTACAACCCCATTAGAAATGCCCCCCTACCCAAAACAGAAATGACAGCAGCTGCTTACAGAGCTGCAACTTCAGGCAACCCTCAATTTGTACAAGTTCCTACAGCCCAGCATCAGCAACAATATGTCACTTACTCTCAGATCCACCATCCTTCCCAATCCATGGCCCCTAATTCTGCTGCCCCTGCCAATTATGCCTATGAATTTGCTGATCCTGCACATGCTCAAATATACTACTCTCAGCCTATGGCGCCCACAATGCCTTCACAGTACCAGACCATGGCAGCTGCTACTCTGATGCAGCCGGAAATTTCTGCACAGCATCCCTCGGATGGCATGAAGCAACAGATAAGAACCTCACAGCAGCTATAAGCAAGTCTACATAAAAGAAACAATTTTGAAAAATTGGTTTGGTTTCCTTTTAAGTTTTATATATGCAGTTGTCGTTGGGTCGTCGTGTCTGGTACATGTTTGTGGTATGTATTTGCCATAGAAAATAAGAAACTGTGATCATCTTGTACTCTTTCTTGTATTTCATAATTCATAGGAAAGGAAATCTGTCCAAGGTTATTATACTGTATAAGTCGAAGTCTGAAACATTATATTGTGAAGTTCAAATTTTAATTCCTGAAATGATTGTCATATAATTTTGGGGGNAGNAACGG
TARGET=135,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03092 Ps_contig_mira-and-tgicl-ass_franssen_28555
SEQUENCE=CGTTCTACGGTCCCGGCGGAGCCTACGCTATGTTCGCCGGCAGAGACGCGAGCAGAGCACTGGCGAAGATGAGCAAAACGAAGAAGACATCACCTCGTCACTGGATGGGCTCACCGAGAAAGAGATCGGAGTTCTCAATGATTGGGAAACCAAGTTCGTCGCTAAGTACCCTGTTGTTGCAACCCTAGTTAATTAATTAATTCAACTCTATAATCTCTATCATGAATCATCTTATTCTGGAGTGTGTTTGTATGAATATGTTGTTGAACTTCCCCTTTGTTTCGATAATAATTAAGTAACTTTTACTTCTAAGGTAAAGGGGTTTTGTTGTTTAAATTTTAAATGGTACCTTTACGAAGGGTTAAATTAGGTTAAA
TARGET=189,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4141
PRIMER_LEFT_PENALTY=0.159680
PRIMER_RIGHT_PENALTY=0.254467
PRIMER_LEFT_SEQUENCE=AAGTTCGTCGCTAAGTACCCTG
PRIMER_RIGHT_SEQUENCE=CAAAGGGGAAGTTCAACAACAT
PRIMER_LEFT=151,22
PRIMER_RIGHT=278,22
PRIMER_LEFT_TM=59.840
PRIMER_RIGHT_TM=60.254
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=0.4141
PRIMER_LEFT_1_PENALTY=0.159680
PRIMER_RIGHT_1_PENALTY=0.254467
PRIMER_LEFT_1_SEQUENCE=CAAGTTCGTCGCTAAGTACCCT
PRIMER_RIGHT_1_SEQUENCE=CAAAGGGGAAGTTCAACAACAT
PRIMER_LEFT_1=150,22
PRIMER_RIGHT_1=278,22
PRIMER_LEFT_1_TM=59.840
PRIMER_RIGHT_1_TM=60.254
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.4291
PRIMER_LEFT_2_PENALTY=0.174657
PRIMER_RIGHT_2_PENALTY=0.254467
PRIMER_LEFT_2_SEQUENCE=GAAACCAAGTTCGTCGCTAAGT
PRIMER_RIGHT_2_SEQUENCE=CAAAGGGGAAGTTCAACAACAT
PRIMER_LEFT_2=145,22
PRIMER_RIGHT_2=278,22
PRIMER_LEFT_2_TM=59.825
PRIMER_RIGHT_2_TM=60.254
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=0.4755
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.254467
PRIMER_LEFT_3_SEQUENCE=CTCAATGATTGGGAAACCAAGT
PRIMER_RIGHT_3_SEQUENCE=CAAAGGGGAAGTTCAACAACAT
PRIMER_LEFT_3=133,22
PRIMER_RIGHT_3=278,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=60.254
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.5243
PRIMER_LEFT_4_PENALTY=0.159680
PRIMER_RIGHT_4_PENALTY=0.364634
PRIMER_LEFT_4_SEQUENCE=CAAGTTCGTCGCTAAGTACCCT
PRIMER_RIGHT_4_SEQUENCE=GAAACAAAGGGGAAGTTCAACA
PRIMER_LEFT_4=150,22
PRIMER_RIGHT_4=282,22
PRIMER_LEFT_4_TM=59.840
PRIMER_RIGHT_4_TM=60.365
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_03093 Ps_contig_mira-and-tgicl-ass_franssen_28556
SEQUENCE=ATNCGARGGAAGGAAGGAATGAAAAGATAAAAGGACGAGCGGTTGAAATTGAAACTCGATAGAAAGGTGAAACCATAGCGAAACCTTGCTTCTTTGTGCGGTGTGAGATTCAAGAAGAAAAATGGAGATTACAACGCAGCAACTCAGCGCATACAACGGCGCAGATCCATCGAAGCCCATATACGTTGCAGTGAAGGGTCGCGTGTTCGACGTCACCACCGGCAAATCGTTCTACGGTCCCGGCGGAGCCTACGCTATGTTCGCCGGCAGAGAACGCGAGCAGAGCACTGGCGAAGATGAGACAAAAACGAAGAAGACATCACCTCGTCACTGGATGGGCTCACCGAGAAAGAGATCGGAGTTCTCAATAGATTAGGGAAACCAGGTTCGTCGCTAAGTACCCTGTTTGTTNCAACCTAGTATATTATNACTNATAATCTCTATC
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03094 Ps_contig_mira-and-tgicl-ass_franssen_28559
SEQUENCE=CCTATCCCCTCGTGTGCCTTGCCTATCCCCTATCCCTGCTCAGGAAACCAAGTTCGTCGCTAAGTACCCTGTTGTTGCAACCCTAGTTAATTAATTAATTCAACTCTATAATCTCTATCATGAATCATCTTATTCTGGAGTGTGTTTGTATGAATATGTTGTTGAATCCCCTTTGTTTCGATAATAATTAGAACTTTTACTTTCTAGAAAGGGGTTTTGTGTTAATTTAATGCTTCAGGTAATGTAAAATGATGTTATACTTATCGTTTGAATCATTCAATAAATTAACATTGGTGTGATTGAATTARGGTTTATGTATTACAAATATATACGAATTATGTCTTGGTAAGTAAA
TARGET=87,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03095 Ps_contig_mira-and-tgicl-ass_franssen_28561
SEQUENCE=TTCGGGGATATATCTCATCTTAATTCTTATCCATCTTCTATCTCATAAAATAAAAAGCGAAGCAGAGAGAGAGAGAGTATCTGCTGAACAAACCATAGAGATATTAGCATGGCCATGGCGTCTGTGGCTCAGCAACTCACGGGTGTAAAATGGACCCCTCTTCTGTGGAAACCAACTCTAGCGCAAAAGCAAAGACGCAGTGGTGTTCTTCCTATTGTATGTTCCATTGCAATTTCAAGTGCAAATAACAAAGAAAGAGCAAACTTGAAACAACTCTTTGAAGATGCTTATGAGAGGTGCCGCACTGCTCCAATGGAGGGTGTTTCGTTTACTCTTGATCAGTTTACTGGCGCTATAGAGAACTATGACTTTGAGTCTGAAATTGGGGCAAAGATTATCGGCACTGTGTTTGCCACTGAAACTAGTGGAGCTTTTGTTGACATTACTTCAAAGTCCACTGCTTACTTGCCGTTGCGAGAGGCATGCATCCGTCAGATTAAGCATGTAGAAGATGTGGGCATAGTTTCTGGCGTGAAAGAGGAATTTGTAATCATTGGTCAAAATGAAGTTGATGATGGCATGATCTTGAGTTTAAGATCTCTCCAGTATGACCTTGCGTGGGAACGTTGTAGACAACTTCAAGCAGAAGATGCTGTTGTGAAGGGTAAGATTATTGATGCAAATAGAGGTGGTGTGGTGGCTGAAGTTGAAGGCCTTAAAGGATTTGTTCCGTTCTCGCAGATGTCAACGAAATCACCGGGAGAAGAGGTTATTGAGATGGAGATTCCTCTAAAGTTTGTGGAGGTGGATGAGGAACAGTCCAGACTTGTCCTTAGCCACCGCAAAGCCGTGGCCGGAATCCAGGGACAATTAGGAATTGGATCAGTAGTTGCTGGCACTGTGCAGAGTCTAAAGCCATATGGTGCTTTCGTTGACATTGGTGGAATCAGTGGTCTCCTTCATGTCAGTCAGATCAGTCATGATCGGATATCGGATATTGAAACTGTTCTTCAACCTGGTGATGTTTTGAAGGTGATGATCTTAAGTCATGACCGAGAGAGAGGCCGAGTAAGTCTTTCAACGAAGAAGTTGGAACCTACACCAGGAGACATGATTCGAAACCCAACTCTTGTGTTCGAGAAGGCGGAGGAGATGGCTCAGACGTTCAGACAGAGAATAGCGCAAGCAGAAGCTATGGCTCGTGCTGATATGCTTAGGTTCCAGCCAGAGAGTGGATTAACGCTAACCAGCGACGGAATCTTAGGACCATTTGGTTCAGATTTGCCTGAAGAGGGACTGGATCTAAGTGAAATACCCCCAGCTGAAGATTAATGATCGAGCGAATTCAGAGGTCTTAACTTTTTTTTGTCTTTTGTTACACAATTTGATCTATGTATTGTTGTAGAATGCTAAACCTAATGTTATATTTTCCTTCTGTTTTGATGTATTGATCTTTCCTCGTCATTGTAAAATTGAAAAAATATATATAATCTATTACTATGTATCTATTTGAGGAAAAATAGATAAAATAAT
TARGET=64,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03096 Ps_contig_mira-and-tgicl-ass_franssen_28566
SEQUENCE=TCCGCCAACCTAATAATCAAAGACGACGGCCCCGACGACATTCTTTCCCTCCAAAACCGCCGTTATGACTTCGCTCCTCTCCTCAACTTCCTCTCAAACGATTCCAACTCCAACAACAACAACAACAACACAGACTCGCTCTCTCCCACTTCCCTCGACTCAACCGAGTTCCAACTCGCCGAGTCATACCGAGCCGTTCCAGCTCCACTCTGGCACGCGCTTCTCAAATCCCTCTGCTCCTCTACCTCCTCCATTAACCTAGCATACGCCGTCGTTTCATGGCTCCAGAAACACAACCTCTGTTTCTCCTACGAGCTTCTCTACTCAATCATCATCCACGCGCTTGGACGAAACGAAAAGCTCTACGAAGCTTTCTTACTCTCTCAACGACAGGTTCTAACTCCGTTAACCTATAATGCATTAATCGGTGCTTGTGCTCGTAACGGTGACCTAGAAAAAGCGCTTAACCTAATGTCTAGAATGCGCAGTGACGGTTTTCAACCCGATTTTGTCAATTATAGTTCAATTATCAAATCCCTAACTCGTTCCAATCGAATCGATTCTCCGGTTCTTCAAAAGCTTTATGCTGAAATCGAATCGGATAAAATTGAAGCCGATGGTCACCTATTGAATGATATAATCCTCGGTTTTTCGAAAGCCTGCGATGCAACACGTGCAATGCATTTTCTTGCTGTTGCTCAGGGTAAAGGTTTGAGCCCTAAATCAGGAACTCTTGTTGCGGTTATATTAGCTTTGGGAAATTCGGGTAGAACGGTTGAGGCAGAGGCGTTGTTTGAGGAGATTAAAGAAAATGGTTTGGAACCGAGAACTAGGGCTTATAATGCATTGCTGAAAGGTTATGTGAAAACTGGTTCTCTTAAAGATGCAGAGTTTGTTGTTTCTGAAATGGAGAAGTGTGGTGTTTTGCCTGATGAACATACTTATAGTTTGTTGGTTGATGCTTATTCTCAGGCTGGAAGATCGGAGAGTGCAAGGATTATGTTGAAAGAAATGGAAGCGAGTAATTTGCAGCCGAATTCGTTTATTTATAGTAGAATATTGGCGAGTTATAGGGATAAAGGGGAGTGGCAGAAATCGTTTCAGGTTTTGAAGGAGATGAAGAGTTGTGGTGTGCAGCCTGATAGACATTTTTATAATGTGATGATTGATACTTTTGGAAAGTATAATTGTCTCGATCATGCGACGGCGACATTTGAACGAATGATGTCTGAGGGGATTCGACCGGACACAGTTACATGGAATACCTTGATTGATTGTCATTGTAAGTCTAGTCGACATAATAGGGCGGAAGAATTGTTTGAGGAAATGCAGCAGAGTGGGTATTCGCCTTGTGTTACGACTTATAACATTATGATTAATTCTATGGGTGCACAGGAAAGATGGGAACGAGTGAGTGATTTGTTGAGCAGAATGCAGAGTCAAGGATTGATGCCCAATGCTGTTACCTATACAACTCTTGTCGATATTTATGGAAAATCTGGAAGGTTTGATGATGCCATCGAGTGCCTCGAGGTGTTGAAATCTATGGGATTCAAGCCAACTTCGACCATGTATAATGCCCTAATTAATGCCTATGCACAGAGGGGTCTGTCCGATCAAGCAGTAAATGCATTTAGGAAGATGGCAGCTGAGGGTCTTACTCCCAGTCTTTTAGCTCTCAATTCCTTAATCAATGCTTTTGGTGAAGATAGAAGAGATGCTGAAGCCTTTGCTGTACTGCAGTACATGAAAGACAATGATGTTGAACCTGATGTAGTTACTTATACCACACTCATGAAAGCTTTGATTCGTGTTGACAAGTTCCGCAAGGTTCCAGCAGTGTATGAAGAAATGGTTAGGTCTGGATGCACTCCAGATAGAAAAGCTAGAGCAATGCTGCGGTCTGCTCTTAGATACATGAAACAGACCCTAAGATCATAAATTATTAAGCACATTAATAAAGCCTATACAGAGCAGTAATCGGTAAAATAAAGTTGATTTTGGTCACCTGGTATATGTGGTTCTAATTCTGAAGCTGCGGTTTAGTATAGCTGGTGTCGCAGAAATATCTGACAGAAGGACAAACGAAGTCTCCAAAATCACATGGAAGTCTCCGAAATCAAAAAAATGAAAAAACATGCAGAGACACTATTGACTTCTCCTAATGTTGTACTATCTGGTATCAGTCTTGGGGATAAATGTATTATATGCAATGTATTGTATGTTTAATGTAACTTATGTAAGTAAAGTTTATACATTAAAGTACATGTATTTCATGTTCATAAGTGGGAGTCCCTGTAAATTAGAAAAAGGAATAGTGAGTGGGAATTCATTTCAATCTCATCCTCAATGCTGCACTCAAGTACATTTGAAACCTTTTATTTACAGTTTATATAGGTTTATCTTCTATCATTTTGTTCAAAGAAAAAATGTTTCAGGCC
TARGET=111,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0199
PRIMER_LEFT_PENALTY=0.010077
PRIMER_RIGHT_PENALTY=0.009871
PRIMER_LEFT_SEQUENCE=CCTAATAATCAAAGACGACGGC
PRIMER_RIGHT_SEQUENCE=GCGTATGCTAGGTTAATGGAGG
PRIMER_LEFT=8,22
PRIMER_RIGHT=268,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.010
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.1000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.0199
PRIMER_LEFT_1_PENALTY=0.010077
PRIMER_RIGHT_1_PENALTY=0.009871
PRIMER_LEFT_1_SEQUENCE=CCTAATAATCAAAGACGACGGC
PRIMER_RIGHT_1_SEQUENCE=GGCGTATGCTAGGTTAATGGAG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=269,22
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=60.010
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.0223
PRIMER_LEFT_2_PENALTY=0.012411
PRIMER_RIGHT_2_PENALTY=0.009871
PRIMER_LEFT_2_SEQUENCE=CCTCTCCTCAACTTCCTCTCAA
PRIMER_RIGHT_2_SEQUENCE=GCGTATGCTAGGTTAATGGAGG
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=268,22
PRIMER_LEFT_2_TM=59.988
PRIMER_RIGHT_2_TM=60.010
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.0223
PRIMER_LEFT_3_PENALTY=0.012411
PRIMER_RIGHT_3_PENALTY=0.009871
PRIMER_LEFT_3_SEQUENCE=CCTCTCCTCAACTTCCTCTCAA
PRIMER_RIGHT_3_SEQUENCE=GGCGTATGCTAGGTTAATGGAG
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=269,22
PRIMER_LEFT_3_TM=59.988
PRIMER_RIGHT_3_TM=60.010
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=0.0268
PRIMER_LEFT_4_PENALTY=0.016950
PRIMER_RIGHT_4_PENALTY=0.009871
PRIMER_LEFT_4_SEQUENCE=TTATGACTTCGCTCCTCTCCTC
PRIMER_RIGHT_4_SEQUENCE=GGCGTATGCTAGGTTAATGGAG
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=269,22
PRIMER_LEFT_4_TM=59.983
PRIMER_RIGHT_4_TM=60.010
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_03097 Ps_contig_mira-and-tgicl-ass_franssen_28577
SEQUENCE=TCGGNGGCTATTCTCGACAGTTCTCACTCACTCGCTTTACGCAACAACAACAAAACGCTCTTTGCTCTCTCTCTTTCCTCAACTCCGCGGCTTAGATCTGAGGAGTTTTGGAAACCCTAATCCACCGTTCTCGTCATCAACCTCACCGCGCTTTTTCTCTCCGATCATCTACTTTTTCACATCCTTAAGTCATGGCAGGAGTTGCACCAGAGGGTTCTCAGTTTGATGCTAAGCAATTTGATACCAAAATGAATGAGTTACTCACCACTGAAGGACAGGACTTCTACACATTTTATGAGGAGGTTTATGATAGTTTTGATGCTATGGGCTTGCAAGAGAATCTTCTCAGAGGAATTTATGCATATGGTTTTGAGAAGCCATCAGCTATTCAGCAAAGGGGAATAGTTCCATTTTGCAAGGGACTTGATGTTATACAACAGGCTCAGTCTGGAACGGGAAAGACCGCTACCTTCTGCTCTGGGATTCTGCAGCAACTTGACTACAGTGTAACAGAATGCCAGGCCTTGGTCTTGGCACCAACACGTGAGCTTGCTCAGCAGATTGAGAAGGTTATGCGGGCACTTGGAGATTATCTAGGTGTGAAGGTGCATGCTTGTGTGGGAGGGACCAGTGTTCGTGAGGACCAACGTATTCTGTCAAGTGGTGTTCATGTTGTTGTAGGTACCCCTGGTCGTGTATTTGACATGCTGCGCAGACAGTCGCTTAGGCCAGATTACATCAAGATGTTTGTATTAGATGAGGCTGATGAAATGCTTTCTCGTGGTTTTAAAGACCAGATCTACGATATATTCCAGCTGTTGCCATCTAAGATTCAAGTGGGGGTTTTCTCTGCCACAATGCCTCCTGAGGCGCTTGAGATCACAAGAAAGTTCATGAACAAACCTGTGAGGATTCTTGTGAAGCGTGATGAGCTTACCTTGGAGGGTATTAAGCAATTTTATGTCAATGTTGATAAAGAGGAATGGAAGCTTGACACACTCTGCGATCTTTATGAGACATTGGCTATCACCCAGAGTGTCATTTTTGTGAACACCAGACGGAAAGTTGATTGGCTAACTGACAAGATGCGAAGCCGTGACCATACAGTCTCAGCAACACACGGAGACATGGACCAGAATACTAGAGATATTATTATGCGGGAATTCCGTTCTGGATCTTCCCGTGTTTTAATAACTACTGATCTTCTGGCTCGTGGTATTGATGTGCAGCAAGTGTCTTTAGTTATAAATTATGATCTCCCCACACAACCCGAAAACTATCTCCATCGTATTGGTCGTAGTGGAAGGTTTGGTAGGAAAGGTGTTGCAATTAACTTTGTCACGAAGGATGATGAAAGAATGCTGGGTGACATCCAGAAGTTCTACAATGTGTTGATTGAGGAGCTTCCTTCCAATGTGGCTGAACTTCTGTGATGAAATTTTTCTTCCATCATACTAGAAGGAAGTTGGTTTCTGAGGTAAGTTATATGTAATGTTTCCCACCTTTTTGCCCTGTTTGTTGTATTTCTGTTTTGAAACCTTGTTTGGCCTTTTTAATAGGTGTAGTAATTTTATTTACATTTTCAGTTGTCCAAACATCCTAAGTGATAAGTACTTACAACGTGGAATCCATTATTGTCTGAATTGGTGTATTGATGGTTGTTTCACCTTTCTAGAAAGGAAGTTTTTATTTTTAAATCGAAGTTTTAATTATTCTAATATATGCTTTGAGCTAGTATTGGTTTTTTGGTATGTAAGCAAAGCTATATCGCCGGCATGGCTGACATCCATAAAGTGTATTTATCTCGGCATGGCCACATGATATAAG
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3049
PRIMER_LEFT_PENALTY=0.174518
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=NGGCTATTCTCGACAGTTCTCA
PRIMER_RIGHT_SEQUENCE=AACTCCTGCCATGACTTAAGGA
PRIMER_LEFT=4,22
PRIMER_RIGHT=202,22
PRIMER_LEFT_TM=60.175
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.3376
PRIMER_LEFT_1_PENALTY=0.207235
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=GNGGCTATTCTCGACAGTTCTC
PRIMER_RIGHT_1_SEQUENCE=AACTCCTGCCATGACTTAAGGA
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=202,22
PRIMER_LEFT_1_TM=59.793
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.3647
PRIMER_LEFT_2_PENALTY=0.234379
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=CTCGACAGTTCTCACTCACTCG
PRIMER_RIGHT_2_SEQUENCE=AACTCCTGCCATGACTTAAGGA
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=202,22
PRIMER_LEFT_2_TM=60.234
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.4448
PRIMER_LEFT_3_PENALTY=0.207235
PRIMER_RIGHT_3_PENALTY=0.237561
PRIMER_LEFT_3_SEQUENCE=GNGGCTATTCTCGACAGTTCTC
PRIMER_RIGHT_3_SEQUENCE=TCCTGTCCTTCAGTGGTGAGTA
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=279,22
PRIMER_LEFT_3_TM=59.793
PRIMER_RIGHT_3_TM=59.762
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.5483
PRIMER_LEFT_4_PENALTY=0.174518
PRIMER_RIGHT_4_PENALTY=0.373775
PRIMER_LEFT_4_SEQUENCE=NGGCTATTCTCGACAGTTCTCA
PRIMER_RIGHT_4_SEQUENCE=TAGGGTTTCCAAAACTCCTCAG
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=118,22
PRIMER_LEFT_4_TM=60.175
PRIMER_RIGHT_4_TM=59.626
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_03098 Ps_contig_mira-and-tgicl-ass_franssen_28577
SEQUENCE=TCGGNGGCTATTCTCGACAGTTCTCACTCACTCGCTTTACGCAACAACAACAAAACGCTCTTTGCTCTCTCTCTTTCCTCAACTCCGCGGCTTAGATCTGAGGAGTTTTGGAAACCCTAATCCACCGTTCTCGTCATCAACCTCACCGCGCTTTTTCTCTCCGATCATCTACTTTTTCACATCCTTAAGTCATGGCAGGAGTTGCACCAGAGGGTTCTCAGTTTGATGCTAAGCAATTTGATACCAAAATGAATGAGTTACTCACCACTGAAGGACAGGACTTCTACACATTTTATGAGGAGGTTTATGATAGTTTTGATGCTATGGGCTTGCAAGAGAATCTTCTCAGAGGAATTTATGCATATGGTTTTGAGAAGCCATCAGCTATTCAGCAAAGGGGAATAGTTCCATTTTGCAAGGGACTTGATGTTATACAACAGGCTCAGTCTGGAACGGGAAAGACCGCTACCTTCTGCTCTGGGATTCTGCAGCAACTTGACTACAGTGTAACAGAATGCCAGGCCTTGGTCTTGGCACCAACACGTGAGCTTGCTCAGCAGATTGAGAAGGTTATGCGGGCACTTGGAGATTATCTAGGTGTGAAGGTGCATGCTTGTGTGGGAGGGACCAGTGTTCGTGAGGACCAACGTATTCTGTCAAGTGGTGTTCATGTTGTTGTAGGTACCCCTGGTCGTGTATTTGACATGCTGCGCAGACAGTCGCTTAGGCCAGATTACATCAAGATGTTTGTATTAGATGAGGCTGATGAAATGCTTTCTCGTGGTTTTAAAGACCAGATCTACGATATATTCCAGCTGTTGCCATCTAAGATTCAAGTGGGGGTTTTCTCTGCCACAATGCCTCCTGAGGCGCTTGAGATCACAAGAAAGTTCATGAACAAACCTGTGAGGATTCTTGTGAAGCGTGATGAGCTTACCTTGGAGGGTATTAAGCAATTTTATGTCAATGTTGATAAAGAGGAATGGAAGCTTGACACACTCTGCGATCTTTATGAGACATTGGCTATCACCCAGAGTGTCATTTTTGTGAACACCAGACGGAAAGTTGATTGGCTAACTGACAAGATGCGAAGCCGTGACCATACAGTCTCAGCAACACACGGAGACATGGACCAGAATACTAGAGATATTATTATGCGGGAATTCCGTTCTGGATCTTCCCGTGTTTTAATAACTACTGATCTTCTGGCTCGTGGTATTGATGTGCAGCAAGTGTCTTTAGTTATAAATTATGATCTCCCCACACAACCCGAAAACTATCTCCATCGTATTGGTCGTAGTGGAAGGTTTGGTAGGAAAGGTGTTGCAATTAACTTTGTCACGAAGGATGATGAAAGAATGCTGGGTGACATCCAGAAGTTCTACAATGTGTTGATTGAGGAGCTTCCTTCCAATGTGGCTGAACTTCTGTGATGAAATTTTTCTTCCATCATACTAGAAGGAAGTTGGTTTCTGAGGTAAGTTATATGTAATGTTTCCCACCTTTTTGCCCTGTTTGTTGTATTTCTGTTTTGAAACCTTGTTTGGCCTTTTTAATAGGTGTAGTAATTTTATTTACATTTTCAGTTGTCCAAACATCCTAAGTGATAAGTACTTACAACGTGGAATCCATTATTGTCTGAATTGGTGTATTGATGGTTGTTTCACCTTTCTAGAAAGGAAGTTTTTATTTTTAAATCGAAGTTTTAATTATTCTAATATATGCTTTGAGCTAGTATTGGTTTTTTGGTATGTAAGCAAAGCTATATCGCCGGCATGGCTGACATCCATAAAGTGTATTTATCTCGGCATGGCCACATGATATAAG
TARGET=65,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3049
PRIMER_LEFT_PENALTY=0.174518
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=NGGCTATTCTCGACAGTTCTCA
PRIMER_RIGHT_SEQUENCE=AACTCCTGCCATGACTTAAGGA
PRIMER_LEFT=4,22
PRIMER_RIGHT=202,22
PRIMER_LEFT_TM=60.175
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.3376
PRIMER_LEFT_1_PENALTY=0.207235
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=GNGGCTATTCTCGACAGTTCTC
PRIMER_RIGHT_1_SEQUENCE=AACTCCTGCCATGACTTAAGGA
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=202,22
PRIMER_LEFT_1_TM=59.793
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.3647
PRIMER_LEFT_2_PENALTY=0.234379
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=CTCGACAGTTCTCACTCACTCG
PRIMER_RIGHT_2_SEQUENCE=AACTCCTGCCATGACTTAAGGA
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=202,22
PRIMER_LEFT_2_TM=60.234
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.4448
PRIMER_LEFT_3_PENALTY=0.207235
PRIMER_RIGHT_3_PENALTY=0.237561
PRIMER_LEFT_3_SEQUENCE=GNGGCTATTCTCGACAGTTCTC
PRIMER_RIGHT_3_SEQUENCE=TCCTGTCCTTCAGTGGTGAGTA
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=279,22
PRIMER_LEFT_3_TM=59.793
PRIMER_RIGHT_3_TM=59.762
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.5483
PRIMER_LEFT_4_PENALTY=0.174518
PRIMER_RIGHT_4_PENALTY=0.373775
PRIMER_LEFT_4_SEQUENCE=NGGCTATTCTCGACAGTTCTCA
PRIMER_RIGHT_4_SEQUENCE=TAGGGTTTCCAAAACTCCTCAG
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=118,22
PRIMER_LEFT_4_TM=60.175
PRIMER_RIGHT_4_TM=59.626
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_03099 Ps_contig_mira-and-tgicl-ass_franssen_28580
SEQUENCE=ACGTTTTTAGTTAGCCAATATGAGGGCTTATTTTTGAACGGGAAATCTATAGTAAGAATNRNACTTTTTACNAAATAAATAAATTTACATTACCACGTAAGCCTTTCAAGAGCAATCATGTAAACTTCCACACAAAAAATAGATGGAAAAACCTTTTTGAGCAAGCAAGTTATCGACAGAGATACACAAGACAAAAACCAAATGTGCATCAAAATATACATGACTTTCTCTGCCATGTGTACAGCTCACAAAAATAAATAACAAAAGGAAAAGGAAAATTACAACATTGCACATATCAACAAAATTATAATCTAAGGTTGCCCAAATATGTTAATTTTCCAAAGGCATCACAGGGGACGATGAGACCTTATCATTATCGTCATCAGAAGTATATGACTTTATATATGAACGAGCAACATCTAACATCACATCGACTTCTTTTTTGTACTCATTGACTGCCCCTGACTTTATTTTGGATTGCATCACACACCATTTCTTGAAATGTGCACCCACTCTTATGTCCAGAGCACATGAACTTCCATTGGTTGTCTCCAAACTCCATTTACAGTGCACCTCAAAATAGGACCCAAATGGAACATCATGCGCCTGTTGCACAGTCTCAAACACCAGGTGTTTCTTGTCTGGTGATAGAACAACATGTTGCCACTCTGTCATGGCTATGTCAGGAGGGCACAGTAGGGCTGTTAACAAAGGGNTCGTAGGTAAGGTTATCTCCGTCGGTACTTGAACC
TARGET=73,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03100 Ps_contig_mira-and-tgicl-ass_franssen_28585
SEQUENCE=CGGGACCACATTGCATTGCACTTTGTGTAGCCGAGAGAAAGAGAAACTTCAAGAGAATTTCTCTTTCTTTCTTCTTCCATCCATTTTCTCTCTACTTTCACGTTTTTGTAAAAGATGGCCGATGCTGAGGATATTCAACCCCTTGTCTGTGACAATGGAACTGGAATGGTGAAGGCTGGATTTGCTGGTGATGATGCTCCGTAGGGCTGTAGTTTCCAAGTATTGTTGGCCRACCTCGTCATAC
TARGET=62,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03101 Ps_contig_mira-and-tgicl-ass_franssen_28586
SEQUENCE=GACCACATTGCATTGCACTTTGTGTAGCCGAGAGAAAGAGAAACTTCAAGAGAATTTCTCTTTCTTTCTTCTTCCATCCATTTTCTCTCTACTTTCACGATGGCCGATGCTGAGGATATTCAACCCCTTGTCTGTGACAATGGAACTGGAATGGTGAAGGCTGGATTTGCTGGTGATGATGCTCCTAGGGCTGTGTTTCCAAGTATTGTTGGCCGACCTCGTCATACTGGTGTCATGGTTGGGATGGGTCAAAAGGATGCCTATGTAG
TARGET=59,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1428
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.012470
PRIMER_LEFT_SEQUENCE=GCCGAGAGAAAGAGAAACTTCA
PRIMER_RIGHT_SEQUENCE=TTCCAGTTCCATTGTCACAGAC
PRIMER_LEFT=26,22
PRIMER_RIGHT=150,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=125
PRIMER_PAIR_PENALTY_1=0.2135
PRIMER_LEFT_1_PENALTY=0.201078
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=ATTGCATTGCACTTTGTGTAGC
PRIMER_RIGHT_1_SEQUENCE=TTCCAGTTCCATTGTCACAGAC
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=150,22
PRIMER_LEFT_1_TM=60.201
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=0.2163
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.085951
PRIMER_LEFT_2_SEQUENCE=GCCGAGAGAAAGAGAAACTTCA
PRIMER_RIGHT_2_SEQUENCE=ACCATGACACCAGTATGACGAG
PRIMER_LEFT_2=26,22
PRIMER_RIGHT_2=238,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=213
PRIMER_PAIR_PENALTY_3=0.2163
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.085951
PRIMER_LEFT_3_SEQUENCE=GCCGAGAGAAAGAGAAACTTCA
PRIMER_RIGHT_3_SEQUENCE=CATGACACCAGTATGACGAGGT
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=236,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=0.2259
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.095538
PRIMER_LEFT_4_SEQUENCE=GCCGAGAGAAAGAGAAACTTCA
PRIMER_RIGHT_4_SEQUENCE=GCCAACAATACTTGGAAACACA
PRIMER_LEFT_4=26,22
PRIMER_RIGHT_4=212,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.904
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_03102 Ps_contig_mira-and-tgicl-ass_franssen_28587
SEQUENCE=ATTTGTAATTTCCTTTTAAGATTTGTCTTAATATTTTTACCCATAGGCAATAAAATTTAACGAGYTATTAATTCATGACGTATAAAAGNCTCAATTGACAAGTTAATTGCGCAAATTGACCAAATTGTAAAATATCTCAATACATGGGCTATAGACATAAACATGAACGTTTCAAAAGGGACAAACCTTCAAATGTAAACTCTGAAAAAAGTGGGCAAAAGGAAATAAAATAAGCATCATCACAATCACTCCTTACTACAGACAACCTAATTAAGAAGACAAGAAGAATTCCAAGGCAAAAAATGCCTCTTAAGTCTGTGCCCTCGACATCCAGAGGACTGCCACAGAACATTTGATAAATCAAACTCAGAATATACCCTCAAATGATCCTATCTCACCTCTTATCCATGGCAACATAGTTCACATGACATGACAGAGACACCAAAAAAGCAACCACATCAAAAATAACTTACCATTATCGCGCAACGTTTTGGGCTTAGAAGCATTTTCTGTGGACAATGGATGGGCCAGACTCATCATATTCACCCTTAGATATCCACATCTGCTGGAAGGTGCTGAGGGATGCAAGGATTGATCCTCCAATCCAGACACTGTACTTCCTCTCTGGTGGAGCCACAACCTTAATCTTCATGCTGCTAGGAGCCAGGGCAGTGATCTCCTTGCTCATACGGTCAGCAATACCGGGAAACATAGTTGAACCACCACTAAGAACAATGTTTCCGTACAGATCCTTTCTGATATCAACATCACACTTCATTATAGAGTTGTAAGTGGTCTCATGAATTCCAGCAGCTTCCATTCCAATCATAGATGGCTGGAAAAGGACTTCTGGGCAACGGAACCTCTCAGCTCCGATTGTGATAACTTGTCCATCAGGAAGCTCATAGTTTTTCTCAATAGAGGAACTGCTCTTTGCAGTTTCAAGCTCTTGTTCATAATCCACAGCAACATAGGCAAGCTTCTCTTTTATGTCACGGACAATTTCCCGCTCAGCCGAGGTGGTGAACATATACCCTCTCTCAGTGAGGATCTTCATCAAAGATTCAGTTAGATCACGACCAGCAAGATCCAAACGAAGGATGGCATGAGGGAGTGCATAACCCTCATAGATTGGCACGGTATGACTCACACCATCACCAGAATCCAAGACAATACCTGTTGTACGACCACTTGCATAGAGGGAGAGGACAGCCTGGATGGCCACATACATGGCAGGTACATTGAAGGTCTCAAACATGATTTGGGTCATCTTTTCTCTGTTGGCCTTTGGGTTGAGTGGAGCCTCAGTTAGAAGCACTGGGTGCTCCTCAGGAGCAACACGCAATTCATTGTAAAATGTGTGATGCCAGATCTTCTCCATGTCATCCCAGTTACTGACTATACCGTGCTCAATGGGGTACTTAAGAGTAAGAATACCTCTTTTAGATTGGGCTTCATCACCTACATAGGCATCCTTTTGACCCATCCCAACCATGACACCAGTATGACGAGGTCGGCCAACAATACTTGGAAACACAGCCCTAGGAGCATCATCACCAGCAAATCCAGCCTTCACCATTCCAGTTCCATTGTCACAGACAAGGGGTTGAATATCCTCAGCATCGGCCATCTTTTACAAAAACGTGAAAGTAGAGAGAAAATGGATGGAAGAAGAAAGAAAGAGAAATTCTCTTGAAGTTTCTCTTTCTCTCGGCTACACAAAGTGCAATGCAATGTGGTCAGAAT
TARGET=1672,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03103 Ps_contig_mira-and-tgicl-ass_franssen_28598
SEQUENCE=CTCTTCTTCTTCTTCTTCTTCTTATAACCCAATCAACAGAGAAAAAGAAAGCAGTTTCAGTTTGAGATTCAGATCGGAGAAAGTGATGGTGATTCATAGAATTGAACTATGCATATCTATGGTTAGAATGGCCATAGAGTTTGTTATGGCAGTTGCAGAAACGGTTGTTATTGTTACAAAGAAAGAACCGCCCGTACCTTTTTTGTTCCGTTCAGCCGTNCAAATCTCCTCTTCCTTTCA
TARGET=2,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03104 Ps_contig_mira-and-tgicl-ass_franssen_28599
SEQUENCE=AATTAGAAATACCYTGAATCAATTCATTCATTCATTCATAATCAACATAACTTGTGCATCATAATCTTGGAAATTAACCAAGAGTTTGAATAAAAACAAACCCTAAAAGCATGGTTGTATCAATTTTCCTTTACATAGTTTGGCATCAAATACAACACGATGCACAGCAAAAGGGAAAATTATGTACTGATCTAAACTATATATACAGAGTTTTATGAAACAGAAATTAACAACACAAACTGAAGAAAAAAACATGCTAGATAGAAATCATCAACGAAGGTAACCGTGAAAAGGAAGAGGAGTATTTGCACGGTTGTACGGAACAAAAGGGTCTGTGTTTCTTTCTTGAACAATAACAACGGTTTCTGCAACTGACATAACAAACTCTATGGCCATTCTAACCATAGAGATGCATAATTCTAATCTATGAATCACCATCACTTTCTCCGATCTGAATCTCAAACTGAAACTGCTTTCTTTTTCTCTGTTGATTGGGTATAAGAAGAAGAAGAGACT
TARGET=22,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03105 Ps_contig_mira-and-tgicl-ass_franssen_28599
SEQUENCE=AATTAGAAATACCYTGAATCAATTCATTCATTCATTCATAATCAACATAACTTGTGCATCATAATCTTGGAAATTAACCAAGAGTTTGAATAAAAACAAACCCTAAAAGCATGGTTGTATCAATTTTCCTTTACATAGTTTGGCATCAAATACAACACGATGCACAGCAAAAGGGAAAATTATGTACTGATCTAAACTATATATACAGAGTTTTATGAAACAGAAATTAACAACACAAACTGAAGAAAAAAACATGCTAGATAGAAATCATCAACGAAGGTAACCGTGAAAAGGAAGAGGAGTATTTGCACGGTTGTACGGAACAAAAGGGTCTGTGTTTCTTTCTTGAACAATAACAACGGTTTCTGCAACTGACATAACAAACTCTATGGCCATTCTAACCATAGAGATGCATAATTCTAATCTATGAATCACCATCACTTTCTCCGATCTGAATCTCAAACTGAAACTGCTTTCTTTTTCTCTGTTGATTGGGTATAAGAAGAAGAAGAGACT
TARGET=500,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03106 Ps_contig_mira-and-tgicl-ass_franssen_28600
SEQUENCE=AATCCCANGTAGGGTCTATAAATTTCAAACAACCACTCTTCCTCGNNGAGTACTCTCTTCTTCTTCTTCTTCTTCTTATAACCCAATCAACAGAGAAAAAGAAAGCAGTTTCAGTTTGAGATTCAGATCGGAGAAAGTGATGGTGATTCATAGAATTGAACTATGCATATCTATGGTTAGAATGGCCATAGAGTTTGTTATGGCAGTTGCAGAAACGGTTGTTATTGTTCAAGAAAGAAACACCGACCCTTTTGTTCCGTTCAGCCGTGCAAATACTCCTCTTCCTTTTCACGGTTATCTTCGTTGATGATTTCCATCTAGCTTGTTTTTTTTGCTTCAGTTTGTGTTGTTAATTTCTGTTTCATAAAACTCTGTACATATAGTTTAGATCAGTACATAATTTTCCCTTTTGGTTTGCATCATGTTGTATTTGATGCAAAACTGTGTAAAGGAAAATTGATAATACCCATGTTTTTAGGGTTTGTTTTTATTCAAACTCTTGGTTAGTTTCCAAGATTATGATGCACAAGTTATGTTGATTATGAATGAAAGAATGAATTGATTCAAGGTATTTCTAATTTCTTAAAACTTCTTGTACTGTTTGCTGGTCCTCTTTGAGAAATCTATGCTTAATTTTCTAAAC
TARGET=56,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2762
PRIMER_LEFT_PENALTY=1.275480
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=TCAAACAACCACTCTTCCTCG
PRIMER_RIGHT_SEQUENCE=ACGAAGATAACCGTGAAAAGGA
PRIMER_LEFT=24,21
PRIMER_RIGHT=303,22
PRIMER_LEFT_TM=60.275
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=1.2791
PRIMER_LEFT_1_PENALTY=1.275480
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=TCAAACAACCACTCTTCCTCG
PRIMER_RIGHT_1_SEQUENCE=CATCAACGAAGATAACCGTGAA
PRIMER_LEFT_1=24,21
PRIMER_RIGHT_1=308,22
PRIMER_LEFT_1_TM=60.275
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=1.2943
PRIMER_LEFT_2_PENALTY=1.275480
PRIMER_RIGHT_2_PENALTY=0.018835
PRIMER_LEFT_2_SEQUENCE=TCAAACAACCACTCTTCCTCG
PRIMER_RIGHT_2_SEQUENCE=GGTCGGTGTTTCTTTCTTGAAC
PRIMER_LEFT_2=24,21
PRIMER_RIGHT_2=247,22
PRIMER_LEFT_2_TM=60.275
PRIMER_RIGHT_2_TM=60.019
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=1.4427
PRIMER_LEFT_3_PENALTY=1.275480
PRIMER_RIGHT_3_PENALTY=0.167242
PRIMER_LEFT_3_SEQUENCE=TCAAACAACCACTCTTCCTCG
PRIMER_RIGHT_3_SEQUENCE=AATCACCATCACTTTCTCCGAT
PRIMER_LEFT_3=24,21
PRIMER_RIGHT_3=147,22
PRIMER_LEFT_3_TM=60.275
PRIMER_RIGHT_3_TM=59.833
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=9.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=1.4483
PRIMER_LEFT_4_PENALTY=1.275480
PRIMER_RIGHT_4_PENALTY=0.172839
PRIMER_LEFT_4_SEQUENCE=TCAAACAACCACTCTTCCTCG
PRIMER_RIGHT_4_SEQUENCE=TTCTGCAACTGCCATAACAAAC
PRIMER_LEFT_4=24,21
PRIMER_RIGHT_4=213,22
PRIMER_LEFT_4_TM=60.275
PRIMER_RIGHT_4_TM=60.173
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_03107 Ps_contig_mira-and-tgicl-ass_franssen_28601
SEQUENCE=CTCTTCTTCTTCTTCTTCTTCTTATAACCCAATCAACAGAGAAAAAGAAAGCAGTTTCAGTTTGAGATTCAGATCGGAGAAAGTGATGGTGATTCATAGAATTGAACTATGCATATCTATGGTTAGAATGGCCATAGAGTTTGTTATGGCAGTTGCAAGGTAAACGGTTTGTTATTGTTACAAAGAAAAGAAACCACCCGTACCCTTTTTGTTCCGTTCAGCCGTGACAAATCTCCTC
TARGET=2,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03108 Ps_contig_mira-and-tgicl-ass_franssen_28602
SEQUENCE=AGTACTATCTCTTCTTCTTCTTATAACCCAATCAACAGAGAAAAAGAAAGCAGTTTCAGTTTGAGATTCAGATCGGAGAAAGTGATGGTGATTCATAGATTAGAATTATGCATCTCTATGGTTAGAATGGCCATAGTAGTTTGTTATGTCAGTTGCAAGTAAACCGTTTGTTATTGTTACAAAGAAAGAACCCACGTAACGTTACCCCTTTTTTGGTTACCCGTAACCAACCGGTGACCTAAAACTTCCCTTCCCCTTTCCTTT
TARGET=10,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03109 Ps_contig_mira-and-tgicl-ass_franssen_28603
SEQUENCE=ATCTCTTCTTCTTCTTATAACCCAATCAACAGAGAAAAAGAAAGCAGTTTCAGTTTGAGATTCAGATCGGAGAAAGTGATGGTGATTCATGGATTAGAATTATGCATCTCTATGGTTANAATGGCCATAAGAGTTTGTTATGTCAGTTGCAGAAACCGTTTGTTATTGTACAAAGAAAGAACACACGTACCCTTTTGTCCGAACACCGTNACAATTCCTCCTTCTTTTCCCTTTTCGAACCGGTTTTACC
TARGET=4,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03110 Ps_contig_mira-and-tgicl-ass_franssen_28604
SEQUENCE=GGCTTTTTGAATGAACCGAACGATTCTGGAATTGGGGTTCCTTGTGAGCTTGTTTCTGTCTAAGTGTAAGGACTCGAGGTTAGGTAACTGGGAGAGTGAACTGGGGATAGAGCCGGTGAGATTGTTGGTGGAAAGATGAAGAAGCTGGAGGTTTTTGAGTTGAGACAAGAAAGAGGGTATGGAGTCTGAGACATTGGTGTATTCGATGAAAAGATATTTGAGGTTTTTGAGCTTGGCTATGGTGGGTTGGATTGGGCCTGTGAGTCTAGGAAGTTTGTTCTCTGAGCTTGCTGGTGCAATTCCTGGAATTGATGAGGCTATGAGCTTTGCTGAGATGTTGAAATTGGTGCAGACAATGGATTATTCTGTCATTGTATTCGACACCGCTCCAACTGGTCATACACTTAGACTGTTGCAATTCCCGTCGGTTTTAGAGAAGGGTCTTGCAAAGATGATGTCTTTGAAAAATAAATTTGGGGGTTTGTTTAGTCAGATGACTCGCATGTTTGGCATGGGTGATGATTTTGGTGAAGATGCACTCCTCGGAAAGCTTGAAGGAATGAAAGATGTAATTGAACAAGTCAATCGGCAATTCAAAGATCCAGACATGACAACCTTTGTCTGTGTTTGCATTCCCGAATTTCTCTCGCTTTACGAAACTGAACGATTGGTTCAAGAACTCACAAAGTTTGAGATTGACACACACAACATCATCATTAATCAAGTAATCTTTGACGATGAAGATGTTGAATCCAAGTTACTTAAAGCAAGAATGAAAATGCAACAGAAGTACTTGGACCAGTTTTACATGTTGTATGATGACTTCAATATTACCAAGCTGCCACTTCTTCCAGAAGAGGTTACTGGTGTTGAAGCTCTGAGATCATTTTCAAGAAATTTCACGCTACCGTATAAACCGTTATGCAGTACTGATCAAGTAGAGCGATTAGAGCGAAGAGTATCAGCGTTACAGAGTCAGTTAAAAGATGCTGAAGAAGAGTTGGAGAAAGTCAAAAGAGGGAAGCAAAAGGCTTGATTTGATTCATTGCTGTGAATCATATGAATAATGCCGTTATTGTAATTTCCTTTTTGTTCACATTTTTGTTGTTGTTGAAGTTTACATGTTATTACGAAACTTCCTTTTATGTGTTGGATTCAAATGTAAGTCATTTATCAGTTTTGTTCTATTTTAATTCATGTAGCTTTTTGTGATGTAAACTGAAAAGAAAAGGATAGAATTGGTGAGTGTAGTTTAAGTTGTTTCGGGAATATTCTTTACTTCTAATAAACCTTGCAATAAAAAAAAGAATTTAAG
TARGET=1102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.1449
PRIMER_LEFT_PENALTY=0.128591
PRIMER_RIGHT_PENALTY=3.016351
PRIMER_LEFT_SEQUENCE=GAAAGTCAAAAGAGGGAAGCAA
PRIMER_RIGHT_SEQUENCE=TCCCGAAACAACTTAAACTACACTC
PRIMER_LEFT=1005,22
PRIMER_RIGHT=1267,25
PRIMER_LEFT_TM=59.871
PRIMER_RIGHT_TM=59.984
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=3.2299
PRIMER_LEFT_1_PENALTY=0.213575
PRIMER_RIGHT_1_PENALTY=3.016351
PRIMER_LEFT_1_SEQUENCE=TTGGAGAAAGTCAAAAGAGGGA
PRIMER_RIGHT_1_SEQUENCE=TCCCGAAACAACTTAAACTACACTC
PRIMER_LEFT_1=1000,22
PRIMER_RIGHT_1=1267,25
PRIMER_LEFT_1_TM=60.214
PRIMER_RIGHT_1_TM=59.984
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=3.2299
PRIMER_LEFT_2_PENALTY=0.213575
PRIMER_RIGHT_2_PENALTY=3.016351
PRIMER_LEFT_2_SEQUENCE=TGGAGAAAGTCAAAAGAGGGAA
PRIMER_RIGHT_2_SEQUENCE=TCCCGAAACAACTTAAACTACACTC
PRIMER_LEFT_2=1001,22
PRIMER_RIGHT_2=1267,25
PRIMER_LEFT_2_TM=60.214
PRIMER_RIGHT_2_TM=59.984
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=3.4950
PRIMER_LEFT_3_PENALTY=0.478666
PRIMER_RIGHT_3_PENALTY=3.016351
PRIMER_LEFT_3_SEQUENCE=AGAAAGTCAAAAGAGGGAAGCA
PRIMER_RIGHT_3_SEQUENCE=TCCCGAAACAACTTAAACTACACTC
PRIMER_LEFT_3=1004,22
PRIMER_RIGHT_3=1267,25
PRIMER_LEFT_3_TM=59.521
PRIMER_RIGHT_3_TM=59.984
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=3.7434
PRIMER_LEFT_4_PENALTY=0.128591
PRIMER_RIGHT_4_PENALTY=3.614777
PRIMER_LEFT_4_SEQUENCE=GAAAGTCAAAAGAGGGAAGCAA
PRIMER_RIGHT_4_SEQUENCE=CCCGAAACAACTTAAACTACACTC
PRIMER_LEFT_4=1005,22
PRIMER_RIGHT_4=1266,24
PRIMER_LEFT_4_TM=59.871
PRIMER_RIGHT_4_TM=58.385
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_03111 Ps_contig_mira-and-tgicl-ass_franssen_28610
SEQUENCE=GTGCTTTTGTGATGTTTGCGGGTTTTGTGGTTTGTGGTTTTCCTTCACAGAGGATGCATTCGAAAGGAGAACAAGGGAACCAGACAAGGTCANTACGTCGGGGGAAACCAGAGCATTTGAAGTGTTTACGGCTGCACTCTCTCTCTCGACTCAATAATGGCTGAAACCGTCGTTGCTGTCCCCGTCGCCGTCGAAGTCGATCCCAATCAAATCGAAGTCAAGCTCTTCAACAGATGGAGCTTCGACGAAGTTCAGCTTAGTGATGTGTCTCTTTCTGATTACATTGGAGTTCAATCAGCAAAACATGCTACATATGTGCCTCACACTGCTGGTAGGTACTCAGTGAAGCGTTTCAGGAAGGCCCAGTGCCCCATTGTTGAGAGAATCACCAACTCTCTCATGATGCACGGCCGAAACAACGGAAAGAAACTCAAGGCTGTTGGGATTATTAAGCATGCCATGGAGATCATTCACTTGCTAACTGATCAAAACCCTATTCAAGTTATTGTTGACGCAGTTGTCAACAGTGGTCCCAGAGAAGATGCAACTAGAATTGGGTCTGCTGGAGTTGTTAGGAGGCAGGCTGTGGATATCTCACCTCTTCGCCGTGTTAACCAGGCCATCTATCTTCTTACAACTGGTGCCCGTGAAGCTGCATTTAGGAACATCAAGACTATTGCTGAATGCTTAGCTGATGAACTTATTAATGCTGCCAAAGGTTCATCCAACAGCTATGCAATCAAGAAAAAGGATGAGATTGAGAGAGTTGCCAAGGCTAATCGTTAAGCAGAATTTGAAAGTAGTCTTAGTGATGAGGAATATTTTATATTGGTCTTTGTATTACATTTGTTTGAATGTTGTTTTAAAACATGGTGTAAACCTTTTCCCGGTTTATTTTTCACATTTTTGTTGAATCAACTTGTTGTGAACAATTTTGCTGTTAGTCTATAACTGTTTTCAAGGATCTATATGATTTCTTACACCATTTATTGTTTTTCCAATTTCAAATCTTTGTGAACCCATTCTCTCTTCTGAAATGAAAGAAAATGCTTATTGANTGT
TARGET=137,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1055
PRIMER_LEFT_PENALTY=0.053348
PRIMER_RIGHT_PENALTY=0.052187
PRIMER_LEFT_SEQUENCE=AGGGAACCAGACAAGGTCANTA
PRIMER_RIGHT_SEQUENCE=TCCTGAAACGCTTCACTGAGTA
PRIMER_LEFT=73,22
PRIMER_RIGHT=357,22
PRIMER_LEFT_TM=59.947
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.9500
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.1980
PRIMER_LEFT_1_PENALTY=0.145769
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=GAGAACAAGGGAACCAGACAAG
PRIMER_RIGHT_1_SEQUENCE=TCCTGAAACGCTTCACTGAGTA
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=357,22
PRIMER_LEFT_1_TM=60.146
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.2183
PRIMER_LEFT_2_PENALTY=0.189072
PRIMER_RIGHT_2_PENALTY=0.029253
PRIMER_LEFT_2_SEQUENCE=AGCATTTGAAGTGTTTACGGCT
PRIMER_RIGHT_2_SEQUENCE=TTGGTGATTCTCTCAACAATGG
PRIMER_LEFT_2=111,22
PRIMER_RIGHT_2=391,22
PRIMER_LEFT_2_TM=60.189
PRIMER_RIGHT_2_TM=59.971
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=281
PRIMER_PAIR_PENALTY_3=0.2353
PRIMER_LEFT_3_PENALTY=0.183145
PRIMER_RIGHT_3_PENALTY=0.052187
PRIMER_LEFT_3_SEQUENCE=GGGAACCAGACAAGGTCANTAC
PRIMER_RIGHT_3_SEQUENCE=TCCTGAAACGCTTCACTGAGTA
PRIMER_LEFT_3=74,22
PRIMER_RIGHT_3=357,22
PRIMER_LEFT_3_TM=59.817
PRIMER_RIGHT_3_TM=60.052
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.75
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.3500
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.2413
PRIMER_LEFT_4_PENALTY=0.189072
PRIMER_RIGHT_4_PENALTY=0.052187
PRIMER_LEFT_4_SEQUENCE=AGCATTTGAAGTGTTTACGGCT
PRIMER_RIGHT_4_SEQUENCE=TCCTGAAACGCTTCACTGAGTA
PRIMER_LEFT_4=111,22
PRIMER_RIGHT_4=357,22
PRIMER_LEFT_4_TM=60.189
PRIMER_RIGHT_4_TM=60.052
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_03112 Ps_contig_mira-and-tgicl-ass_franssen_28641
SEQUENCE=TATCACTTACCCCTTTTTTTTCTCTCCACTTGTTTTCTTCCGATCCAAACATCGCTTTTTCCTCTCCCCACTCTCTCCTTCCTTCCCTTTTCCCTGTTTTCTACCTTCCTCAAATTGCGAAACAAGCCCCCTGTTTCCGTTAAAGCGACCTTCTTTCGATACCCTTTTCATCCAATTGCATAATCCAATACTATGGTTTTCTGATTCAATTCGATTCCATTCTTTTCAATTTCTTAGGTTGCTGAGTTTTTGGCTTGGTTTTATTATGGATAAGTACGAGGCTGTTAAGGATTTGGGTTCTGGCAATTTTGGGGTTGCTAGGCTTATGAGAGACAAAGTTACAAAGGAACTTGTCGCCATGAAATACATCGAGCGTGGCCACAAGATTGATGAGAACGTGGCTAGAGAGATCATGAATCACAGGTCTCTTCGGCATCCGAATATAATTCGATTCAAGGAGGTGATTTTGACTCCAACACATTTAGCAATAGTTATGGAGTATGCAGCTGGAGGAGAGCTTTTTGAGAGGATATGTAATGCTGGAAGGTTCAGTGAAGATGAGGCCAGGTATTTCTTTCAGCAGCTGATCTCAGGTGTCCATTTCTGTCATACCATGCAAATATGCCACAGAGATCTGAAGCTAGAGAATACTCTTTTAGATGGAAGTCCTGCACCCCGCTTAAAAATTTGTGACTTCGGTTACTCCAAGTCATCTTTGCTCCATTCACGTCCCAAATCAACGGTTGGAACACCGGCGTATATAGCACCAGAGGTTCTTTCTAGGAGGGAGTATGATGGAAAGATGGCTGATGTATGGTCATGTGGGGTGACGCTTTATGTGATGCTGGTCGGAGCATATCCGTTTGAGGACCAGGATGACCCTAAAAATTTTAGGAAAACGATTCAGCGTATAATGGCCATTCAATACAGGATTCCTGATTATGTTCACATATCTCAAGATTGCAGACACCTTATCTCTCGGATATTTGTTGCAAATCCATTGAGGAGAGTTAATATTAGAGAAATCAAGAGCCACCCATGGTTTTTAAAGAATCTTCCAAGGGAGCTAACAGAATCAGCTCAAGCTATGTACTACCAGAGGGAAAATCCAAGCTTTCACCTACAAAGCGTGGACGATGTGATGAAAATCGTGGGAGAGGCAAGAAACCCGCCTCCAATATCTAGGCCCATCAAAGGTTTTGGCTGGGAAGGCGAAGAAGATGAAGAAGCGGAAGATTTGGAAGCAGAGTTGGAAGAGGAAGTTGAAGAAGATGAGTACGATAAGAGGGTCAAAGAGGTTCATGCAAGTGGAGAATTCCATATCAGTTAAGCACCCAAATCCTTGCATTATTTGACTAAAATTAATTTTTAGAAGACCTACCCTTTTATTTTATCTTGTTGTAGTAAACTAGAAGTCACCTTATTTGTGTATTATTATTATTATTATGACTTCTAGTGTTATTTTGTATGTGTATCATGAGTCGGGACCGCCGTAACTTAGTAA
TARGET=1430,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0302
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=1.029480
PRIMER_LEFT_SEQUENCE=TCAGTTAAGCACCCAAATCCTT
PRIMER_RIGHT_SEQUENCE=GGTCCCGACTCATGATACACAT
PRIMER_LEFT=1322,22
PRIMER_RIGHT=1488,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=61.029
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=1.0696
PRIMER_LEFT_1_PENALTY=0.040146
PRIMER_RIGHT_1_PENALTY=1.029480
PRIMER_LEFT_1_SEQUENCE=CATGCAAGTGGAGAATTCCATA
PRIMER_RIGHT_1_SEQUENCE=GGTCCCGACTCATGATACACAT
PRIMER_LEFT_1=1300,22
PRIMER_RIGHT_1=1488,22
PRIMER_LEFT_1_TM=59.960
PRIMER_RIGHT_1_TM=61.029
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=1.1730
PRIMER_LEFT_2_PENALTY=0.143555
PRIMER_RIGHT_2_PENALTY=1.029480
PRIMER_LEFT_2_SEQUENCE=AGATGAAGAAGCGGAAGATTTG
PRIMER_RIGHT_2_SEQUENCE=GGTCCCGACTCATGATACACAT
PRIMER_LEFT_2=1218,22
PRIMER_RIGHT_2=1488,22
PRIMER_LEFT_2_TM=59.856
PRIMER_RIGHT_2_TM=61.029
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=1.2402
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=1.239452
PRIMER_LEFT_3_SEQUENCE=TCAGTTAAGCACCCAAATCCTT
PRIMER_RIGHT_3_SEQUENCE=CCCGACTCATGATACACATACAA
PRIMER_LEFT_3=1322,22
PRIMER_RIGHT_3=1485,23
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=59.761
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=1.2494
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=1.029480
PRIMER_LEFT_4_SEQUENCE=GTCAAAGAGGTTCATGCAAGTG
PRIMER_RIGHT_4_SEQUENCE=GGTCCCGACTCATGATACACAT
PRIMER_LEFT_4=1288,22
PRIMER_RIGHT_4=1488,22
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=61.029
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=201
=
PRIMER_SEQUENCE_ID=SSR_03113 Ps_contig_mira-and-tgicl-ass_franssen_28642
SEQUENCE=CGATGTGATGAAAATCGTGGGAGAGGCAARAAACCCGCCTCCAATATCTAGGCCCATCAAAGGTTTTGGCTGGGAAGGYGAAGAAGATGAAGAAGCGGAAGATTTGGAAGCAGAGTTGGAAGAGGAAGTTGAAGAAGATGAGTACGATAAGAGGGTCAAAGAGGTTCATGCAAGTGGAGAATTCCATATCAGTTAAGCACCCAAATCCTTGCATATTTGCTAAAATAATTTTTTAGAAGACACCCTTTATTTTATCTGTTGTGTAAACTAGAAGTCACCTATTTGTGTATATTATTATTATTATGACTCTAGTGATTTTGTATGTGTATCATGATGGACGCCGACTATGAGAACAATGTTAGTCTCATTACATTCTAAATTGCCATGTTTGAGAACAGTAATCTTCTGATTACATGCTAGAATCCATATCAGTTTTCCATATATGTATGTGCTCGCTCACGTGAGTGTGTTTTATTTGAATCACAATAAGAAAAAGTACTTAAGAAAAAAAGGAATAACAATTGAAC
TARGET=290,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03114 Ps_contig_mira-and-tgicl-ass_franssen_28669
SEQUENCE=AAGGTTTTAAAAAAATTAAAGCACTCGAAGCATTATAACAACGTGAAAGAATCTCCACTCCACAGTCCATACAACAACGAGCTCATAATTTACACATAGANANAAAAAANTAAAACAAAGAATGAATACACCGAATAGATAATTCAAAACAACAAGACATGTCAATGATGATGATGGATTCATTAGCCCCTTGTCTTGAAAGGAATTGCTCGAATCACGATCTGGTGATATAGTGCAGCAAGGGCAGCTCCAATAAAGGTCCAACCCAGAATATCCAGTGGTCGTCCCAAGCATGGTCTCTGTTGTATATTATGGCAGCACCAAGACTCCTAGCAGGTTAATACCAGTTCCAGTTATAGGAATAGTAGCCAAGTGAAACCAAAAACNCAGCAAACCATTGGAAGAGGTGACCAAAATCGGAACATGAAGAACTCCCTAGCTATTTCTCTTGGCATCAGTGGACAAGGAAGAAA
TARGET=165,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0622
PRIMER_LEFT_PENALTY=0.028056
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=CTCCACTCCACAGTCCATACAA
PRIMER_RIGHT_SEQUENCE=TCAAGACAAGGGGCTAATGAAT
PRIMER_LEFT=52,22
PRIMER_RIGHT=198,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=147
PRIMER_PAIR_PENALTY_1=0.1238
PRIMER_LEFT_1_PENALTY=0.028056
PRIMER_RIGHT_1_PENALTY=0.095771
PRIMER_LEFT_1_SEQUENCE=CTCCACTCCACAGTCCATACAA
PRIMER_RIGHT_1_SEQUENCE=TATCACCAGATCGTGATTCGAG
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=229,22
PRIMER_LEFT_1_TM=60.028
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.1433
PRIMER_LEFT_2_PENALTY=0.109200
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=TCCACTCCACAGTCCATACAAC
PRIMER_RIGHT_2_SEQUENCE=TCAAGACAAGGGGCTAATGAAT
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=198,22
PRIMER_LEFT_2_TM=59.891
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.1503
PRIMER_LEFT_3_PENALTY=0.028056
PRIMER_RIGHT_3_PENALTY=0.122257
PRIMER_LEFT_3_SEQUENCE=CTCCACTCCACAGTCCATACAA
PRIMER_RIGHT_3_SEQUENCE=CTTTCAAGACAAGGGGCTAATG
PRIMER_LEFT_3=52,22
PRIMER_RIGHT_3=201,22
PRIMER_LEFT_3_TM=60.028
PRIMER_RIGHT_3_TM=60.122
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=150
PRIMER_PAIR_PENALTY_4=0.2050
PRIMER_LEFT_4_PENALTY=0.109200
PRIMER_RIGHT_4_PENALTY=0.095771
PRIMER_LEFT_4_SEQUENCE=TCCACTCCACAGTCCATACAAC
PRIMER_RIGHT_4_SEQUENCE=TATCACCAGATCGTGATTCGAG
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=229,22
PRIMER_LEFT_4_TM=59.891
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_03115 Ps_contig_mira-and-tgicl-ass_franssen_28671
SEQUENCE=CGGGGAACACAGTTAGAGAAAAAAAAATGGAGAGGCAAGAAGATGTTAAGATTGGAGCAAACAAATTCTCAGAAAAGAATGCATTAGGCACAGGAGCTCAGAGTGATAGTAAGGACTACAAGGAAGCACCAGCAGCACCATTGTTTGAGCCAGGGGAGTTGAAGTCATGGTCTTTTTACAGAGCTGGAATTGCTGAATTTGTTGCTAACTTTTTTATTTCTTTACATCACTGTCTTAACTGTCATGGGTGTGAATAGATCATCCTCTAAGTGTGCCTCTGTTGGTATTCAAGGAATTGCTTGGGCTTTTGGTGGTATGATATTTGCACTTGTCTACTGCACTGCTGGAATTTCAGGTGGACACATAAATCCAGCTGTGACATTTGGTTTGTTTTTGGCTAGGAAACTGTCCTTGACAAGAGCAGTATTCTACATAATAATGCAGTGTCTTGGAGCTATCTGTGGTGCTGGTGTGGTGAAGGGTTTTGAGGGTAATGCTCGTTATGAGTTGTTCAAAGGTGGAGCGAATGTTGTGAATCCTGGTTACACCAAGGGGGATGGTCTTGGAGCTGAGATTGTTGGTACTTTTGTTCTTGTTTACACTGTTTTCTCTGCCACTGATGCCAAGAGAAATGCTAGGGATTCTCATGTTCCGATTTTGGCACCTCTTCCAATTGGGTTTGCTGTGTTTTTGGTTCACTTGGCTACTATTCCTATAACTGGAACTGGTATTAACCCTGCTAGGAGTCTTGGTGCTGCCATAATATACAACAGAGACCATGCTTGGGACGACCACTGGATATTCTGGGTTGGACCTTTTATTGGAGCTGCCCTTGCTGCACTATATCACCAGATCGTGATTCGAGCAATTCCTTTCAAGACAAGGGGCTAATGAATCCATCATCATCATTGACATGTCTTGTTGTTTTGAATTATCTATTCGGTGTATTCATTCTTGTTTATTTTTTTATCTATGTGTAAATTATGAGCTCGTGTTGTATGGACTGTGGAGTGGAGATTCTTTCACGTTGTTATAATGCTTCGAGTGCTTATTTTTTTCCTTAAAAGAAAATGTGATATATCTTACTATTATTCAAAAACAATTTATGGTCGATCATACAAAAG
TARGET=898,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0622
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.028056
PRIMER_LEFT_SEQUENCE=CAAGACAAGGGGCTAATGAATC
PRIMER_RIGHT_SEQUENCE=CTCCACTCCACAGTCCATACAA
PRIMER_LEFT=875,22
PRIMER_RIGHT=1015,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.0622
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.028056
PRIMER_LEFT_1_SEQUENCE=TCAAGACAAGGGGCTAATGAAT
PRIMER_RIGHT_1_SEQUENCE=CTCCACTCCACAGTCCATACAA
PRIMER_LEFT_1=874,22
PRIMER_RIGHT_1=1015,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=142
PRIMER_PAIR_PENALTY_2=0.0809
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.046801
PRIMER_LEFT_2_SEQUENCE=TCAAGACAAGGGGCTAATGAAT
PRIMER_RIGHT_2_SEQUENCE=ACTCCACAGTCCATACAACACG
PRIMER_LEFT_2=874,22
PRIMER_RIGHT_2=1011,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.953
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=138
PRIMER_PAIR_PENALTY_3=0.0809
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.046801
PRIMER_LEFT_3_SEQUENCE=CAAGACAAGGGGCTAATGAATC
PRIMER_RIGHT_3_SEQUENCE=ACTCCACAGTCCATACAACACG
PRIMER_LEFT_3=875,22
PRIMER_RIGHT_3=1011,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.953
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=137
PRIMER_PAIR_PENALTY_4=0.1238
PRIMER_LEFT_4_PENALTY=0.095771
PRIMER_RIGHT_4_PENALTY=0.028056
PRIMER_LEFT_4_SEQUENCE=TATCACCAGATCGTGATTCGAG
PRIMER_RIGHT_4_SEQUENCE=CTCCACTCCACAGTCCATACAA
PRIMER_LEFT_4=843,22
PRIMER_RIGHT_4=1015,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_03116 Ps_contig_mira-and-tgicl-ass_franssen_28672
SEQUENCE=TCAACGCAGAGTACGCGGGGGTGTTTAAATCCAAACTCACATTCAAATTCTCCGCTCTCACTCTCTCTTTCTCTCTTTCAAATCCTAAAAACTTAAAGCAAACTTATTGGATTATGAACATGGCTTCTAATTACAGAATTGCCATAACTTTCTCTGCAGTGTTAACCCTCCTTGTACTTTTACCCACACTCACAGTGGGAGAGCTTGTACAAGAACAGCCACTAGTGCTTAAATACCATAACGGCCAACTCCTTAAAGGACGCATCACCGTTAATCTCATTTGGTACGGAACATTCACCCCAATCCAACGATCAATAATCGTCGATTTCATCAACTCTGTAAGCACCACCGGTGCGCCTCTTCCATCAGCCGCGGCATGGTGGAAAACCACCGAGAAATACAAGGTAGGATCCTCTGCGCTAGTTGTAGGGCAACAGTTCCTGCACCCTGCTTACACCCTTGGGAAGAATCTAAAAGGAAAGGATCTTCTTAGTTTAGCCACCAAGTTCAACGAAATTTCTTCCATAACCGTTGTTCTTACAGCCAAAGACGTCAACGTTGACGGTTTCTGTATGAGTCGCTGCGGAACCCACGGCTCTGTTCCACGTGGCAGCGGTGGAGCAAAAACCCCTTACATATGGGTTGGAAACGCGGAGACACTATGTCCCGGACAGTGCGCGTGGCCTTTTCATCAGCCTATTTACGGGCCACAGTCACCGCCGCTGGTTGCACCTAATGGCGACGTAGGTGTTGACGGAATGATCATCAACTTAGCCACGCTTTTGGCCGGTACTGTCACGAATCCCTTTAACAACGGATACTTCCAGGGACCGGCGACGGCGCCGCTAGAAGCAGTTACGGCTTGTACGGGGGTTTTCGGTAGTGGAGCCTACCCGGGTTATGCGGGTCGGGTTCTTGTTGATGGGACTACTGGTTCGAGTTATAATGCGCACGGTGCTAACGGGAGGAGGTATCTTCTGCCCGCAATGTGGGACCCGCAGACTTCAGCATGCAGAACTCTTGTATAAAGAGGGGACCATGAATGGACACGTGCCGTGATATGGTGTATCGGGTTGTATAGATATATATGATGGGTTGATAAATACGAGAGCAGATGAATGCTCTCTGGGACTGACTAGGAGGGGTGTGTGTGTTTTTATATATTCATCTAATACATTGTTCAACTAGTGTATTAAATCGTTTATAAAGATTGTAAATGTACCGTAAACATTGAGGTATGTAAAAAAGTTTGAATAATTTTATTTAAAAACTGTGGTGGTATGAACGTAATTTTATTATGAATGGAAATCTCTCATTTTCTTGC
TARGET=1145,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1720
PRIMER_LEFT_PENALTY=0.099438
PRIMER_RIGHT_PENALTY=1.072607
PRIMER_LEFT_SEQUENCE=ATGCTCTCTGGGACTGACTAGG
PRIMER_RIGHT_SEQUENCE=CGTTCATACCACCACAGTTTTT
PRIMER_LEFT=1118,22
PRIMER_RIGHT=1286,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=58.927
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=1.4275
PRIMER_LEFT_1_PENALTY=0.354881
PRIMER_RIGHT_1_PENALTY=1.072607
PRIMER_LEFT_1_SEQUENCE=GCTCTCTGGGACTGACTAGGAG
PRIMER_RIGHT_1_SEQUENCE=CGTTCATACCACCACAGTTTTT
PRIMER_LEFT_1=1120,22
PRIMER_RIGHT_1=1286,22
PRIMER_LEFT_1_TM=59.645
PRIMER_RIGHT_1_TM=58.927
PRIMER_LEFT_1_GC_PERCENT=59.091
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=1.4641
PRIMER_LEFT_2_PENALTY=0.391473
PRIMER_RIGHT_2_PENALTY=1.072607
PRIMER_LEFT_2_SEQUENCE=TGGGTTGATAAATACGAGAGCA
PRIMER_RIGHT_2_SEQUENCE=CGTTCATACCACCACAGTTTTT
PRIMER_LEFT_2=1091,22
PRIMER_RIGHT_2=1286,22
PRIMER_LEFT_2_TM=59.609
PRIMER_RIGHT_2_TM=58.927
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=1.5730
PRIMER_LEFT_3_PENALTY=0.500367
PRIMER_RIGHT_3_PENALTY=1.072607
PRIMER_LEFT_3_SEQUENCE=CTCTCTGGGACTGACTAGGAGG
PRIMER_RIGHT_3_SEQUENCE=CGTTCATACCACCACAGTTTTT
PRIMER_LEFT_3=1121,22
PRIMER_RIGHT_3=1286,22
PRIMER_LEFT_3_TM=59.500
PRIMER_RIGHT_3_TM=58.927
PRIMER_LEFT_3_GC_PERCENT=59.091
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=1.6790
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=1.579542
PRIMER_LEFT_4_SEQUENCE=ATGCTCTCTGGGACTGACTAGG
PRIMER_RIGHT_4_SEQUENCE=ACGTTCATACCACCACAGTTTT
PRIMER_LEFT_4=1118,22
PRIMER_RIGHT_4=1287,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=58.420
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_03117 Ps_contig_mira-and-tgicl-ass_franssen_28675
SEQUENCE=TGAAAAGATTGGAAAACTTGTTTCTTATGCTACGGTTTAAGTTCACAGCTCAGGTTGAAAAAGGTAAGATTAAGAAACTGAGTGGTGTTAAAACCAAAGAGCTTTTCATTTGGATTACACTTAGTGATATTTATGTTGATGATCCACCTACCGGCAAAATCACTTTCCAAACTCCTGCTGGACTCTTTAAGACTTTTCCAGTTTCTGCTTTTGAAGTTGATGAGAAATCAACTGCTGTGAAAGAGATTAACAACAAAGTCAATGAAACTGTTGCTGGTGTTAAAGAGGTCTAGTTTTAGATCTCTTTATTGATTATTCATGTGATTGGTTGTTGCTGAAATTTCAAGTATTAAGTTTACTTTACTTTACTTTTATCTAGATGTTACTCATATTTATTTAAAATGAAATGAAATTAATTTTAACCTT
TARGET=355,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03118 Ps_contig_mira-and-tgicl-ass_franssen_28686
SEQUENCE=TCTTCGTACATATCATNTCTCTTAACCAATATATCTATCTACCTATGGCTAGATCTTCAGACATATCATTTCTCTGAGATAAAACATCTAACAGTTGGAAGATGGCGATGGAGGGTACTATCCAGTGCGAAGCAAACTACGTTCCTCTCACTCCAATCAGCTTCTTGGAGCGCTCTGCCGTTGTTTACGATAACAACGTCTCAATCTCCTACGGTGATGTCACCTACACATGGTCTCAGACTCATCAACGATGCATCAAACTCGCTTCTTCCATTTCTCAACTCGGCGTTTCTCCTAGTGACGTGGTTGCTGTATTGGCCCCTAATATTCCAGCCATGTATGAATTGCATTTTGGTGTTCCAATGTCGGGGGCTATTCTATGTACACTGAATACTCGTCACGATTCATCCATGGTTTCGGGACTGTTAAAGCATTCTGATGCCAAAGTCCTTTTTGTTGATCATGAGTTACTTGATATTGCTAAGGGAGCACTTCAAATCCTGTCAAAATCAACCTCCAAGCTTCCTATTTTGGTCTTAATTTTGGAGCCTGAGGCTCATAACAACAGTTCAGTCTCTCCAGGAATATTGATCTATGAGAATCTTATAGCTCAAGGGAAACTTGATTTTGAAGTGAAAAGACCAAAGGATGAATGCCATCCAATTGCATTGAATTACACTTCTGGAACCACATCAAGTCCTAAAGGTGTGGTATATAGCCATCGGGGTACCTATCTTAATGCGTTATCTACGATTCTTGTCAACGATATGCCGTCTAAGCCGGTGTATTTGTGGTGTCTTCCCATGTTTCATTGCAACGGATGGTGTTTACCTTGGAGTATTGCTGCTCAAGGTGGCACCAATGTCTGCCAAAGAAATGTAACTGGGGAAGGGATATTTGACAAAGTTTTTAAGCACAAAGTAACCCACATGGCGGGTGCGCCAACAATTCTGAACATGATCCTAAATTCACCGCCCGAAATCAGAAAACCACTTCCGAGGAAGGTGACAATGATGACAGGCGGTGCTCCACCGCCACCGGCTGTGTTCTTTAGGATGGAAGAGCTAGGATTTGAGTTAGTTCATTCATATGGTTTGACAGAAACTTATGGTTCTGCGGCAACTTGCAACTGGAAACCAGAATGGGGTGGCCTGTCTCGAGACGCGCAGGCCAAAATGAAGGCGCGTCAAGGAGTACATCATGTTGGAATTGAAGTACTTGACATAAAAGATCCTGTCACAATGAAGAGTGTACCGGCGGATGCAAAAACCATAGGTGAGGTGATGTTTAGAGGAAACACTGTGGCGAATGGATATTTCAAGAATTTGAAAGCAACACAAGAGGCATTTAAAGGTGGATGGTTTAGGTCCGGTGACTTGGGAGTAATGCACTCAGACGGTTACATAGAACTTAAGGATCGGGCGAAGGATATTATCATCTCTGGGGGAGAAAACATTAGCTCAATTGAGCTGGAAGGTGTGATTTATAGTCATCCATCAGTTGTTGATGTTGCTGTTGTGGGGAGAGCCGATGAATATTGGGGAGAGTCACCGTGTGCATTCGTGAAGCTCAAGGAAGGGTACAATGCAACCGAAGAAGAGATAATACAATTTTGTCGGGAGCGTTTGCCTCGTTTTATGGCTCCTCGAACTGTGGTGTTTTCTGATCTGCCAAAGACATCAACTGGCAAGACACAGAAATTTATATTGAAGGAGAAAGCAAAGGCTATGGGAAGCTTGTCTAAGAAAAAGAACAATAGCAGATTGTAATGCATTGAATGTAATAATATCTTCAACCTCATGTTGTGATTTAATGTTGGTACTTTTCATCTCTATGTAAGATTTATTTATTTGAATAAAGAAAACTTCAATTGGAAATGACTTGAAACTCTTTCTTTTCTATGTAAAGTTCAACTTGTGGAACACAGTAAAACAATAAACAAACTGTTATTAACTAA
TARGET=1841,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03119 Ps_contig_mira-and-tgicl-ass_franssen_28711
SEQUENCE=ATCAATATCAAACATNTTTTTTTCATCCCCTCCAACAGATTTCTCATTATGAAATATTGCTACAAAGATTCCTCTAATGATGAGGATTACCACAAGAAAAAAAGTTCCATATTCCACAGACTAAAAATCTTACAATGTTGAGATCCGCCAACACTAACTTTCAACAGATTTACACAACAAAATTTAACAGTTCAACAAAACTTTGAGCTTTAACTCAAGCAAACACACAAAAGGACCCAAGTTAAAACTTGTACATCACTTGTACAACTGAAAATACAATAAAATACAGTCAATTGTCTGGCCTCAATCTCTTGCTAAATCGATGCAGGGACAAGAGGCCGACCTCGTCCGATTGCAAAACCCCGTGTTCCATCAGGCATTCTTGGCCCAGGAGGCGGTTTTGATGCTTCAGCGGTATGGATAAATGATGTACCTCCATGGCCCATTCCATTTGTAGCACGATATTTGTGCTTTCTCGATCTTGCCTGTTTAGGGTACCTCTGCCCACCTTTATCCTTTGACAAATGCTCCCCATCCTTTTCCTCAAGAATATCTTCATGATGCTCATGTGAGACGTGATTCTCCTCGTCTCTAGCTTTCTCAGATACATGACTTGAGCTATTCTTCTCTGAGTTAGATCCCTTCCAAGCTTGGTTTTTGTGGGCATGTGAGACATGATTCTCCTCATCTCTAGTTTTCTCAGATGCATGACTTGAGCTATTCTTCTCAGAGTTAGATCCCTTCCAAGCTTGTTTTTTGTGGGCATACTTATCCATCCGATTGAGAAATTTAACTCGCATCCCATTTCTCCAGTCTTGTTCATTATTTAACAGTGCCACAGCTTTCTCAGCAGCCTCGACAGTCTCATATTCTACAAGAGCGTGCAACTTGCTACCAAGAAACTTGTCTTGCTTGATATGTTTAGCAGATGCTGCAGTAGAACGCGGGTCATTTATAGTTACCTTTTTTACATTTCCAGCTTTTTGGAATATTTGCCGAATGTTCTCCTTTGAATGGTCCTCTGGCAAATTCTCTGCCAAAACAGTAAATAACTTATGATCTTTCACTTCATTGAGACGAAGTGGATTGAGCCGCTTAACCCTTTTCTCATCACCACTAACAACAAGCAGTGAAGATTCCTTCAGTGCAGCAGCAATAAGTAATTGGTCCCGAGTGAGCTTCTTTGTTTTTCTAAAAGAAGCAATCACTTGTATAGGAACAAACCCTTCCTTGTTCCTTCTGACGAGACTCAGCAAATACTTATCAGTGGGCAGATTTTCATCACTGAAATAATACTCCACCTGCTTAATTATCTTGAGCTTGAGATCCTCCAATGAGACACCAGTCTCAGCCACGTGATCGGGTTCCTGGCCTTCATCATGGTGGTCATGCTCGTGCTCATGGTCGAAATCGTGATTGAGTCCATGTTCATGAACATGATCACGCTCTTCGTCTGAAATCAAAGCATGAACTTCAGCCTCAGCAACGTAATCAAAGTCAACAGGTGGATCGGGAGTAATAACAGCATCACCGAGCGGAGGAGGAGGCGAGGCGGTGGGATCGCCGGCGGTTGGAGCGGTGACGATGGCTGAAGGTCGGCCTTGTTCACCGGTTTCCATTGAGGAGAGAGAGGATGGTGTGTTCGCTCTCTCTCTCTAGAAAATTTCCACCCTTACCCCTTTTTTCTTTTCTTGTTTCTTTCTCTCTCCNCCGCCCCCGYTCTCTGTCGTTGAACTACCCACTGTCCTT
TARGET=1646,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03120 Ps_contig_mira-and-tgicl-ass_franssen_28716
SEQUENCE=TCGGGGGTAGACGAATCAAACGTTAAAACTAGGCCACGTAGGTCTTCCTCTAACCACACCCAATCCTTCATAAATCCACATTCTCACTACTACTCTTGATTCATCACCTTCCTCTTCTCCAAGAGGTTCTGAACTTCCACACACTCTCTCTCTCGTTTCTCTTTCACATTTAAGATGGCAGCATCATCAGCCTGTCTTGTTGGAAATGCTTTATCCACTCATAGTAATGGAGTGTCTCTAGGGAAGGACTTGAATGGAAGATACCTATTCTCATCTTGGAGGTTTTCGCCGATGAATAATAGGGCATCGAAAGCATTTTCTGTAAAGGCAACTTTAGATCAAAGTCAACAGGAAGGTAGAAGGGGGATGCTGAAATTGTTGCTTGGAAATGTTGGTGTTGGTTTACCTGCATTATTTGGARATGGAAAAGCTTATGCTGCTGATGATCAAGGTGTTTCTTCGTCGAGAATGTCTTATTCAAGGTTTCTTGAGTATTTGGACAAGGATAGAGTTACCAAAGTGGATTTATATGAGAATGGAACCATAGCTATTGTTGAGGCTGTTTCTCCTGAGTTAGGAAACCGGTTGCAACGTGTTAGGGTTCAACTTCCTGGACTTAGCCAAGAGCTTCTTCAGAAATTAAGGGAAAAGAACATTGATTTTGCAGCTCATAATAACCAAGAGGATTCGGGTTCTTTTTTATTTAACTTGATTGGGAATCTGGCTTTCCCTCTTGCTGTCATTGGTGTATTGTTCCTTCTCTCGAGGAGATCTGGTGGTATGGGAGGTCCCGGCGGACCTGGATTTCCTCTTCAATTCGGTCAATCTAAAGCCAAGTTTCAAATGGAACCGAACACTGGAGTGACGTTTGATGATGTTGCTGGGGTGGATGAAGCCAAGCAAGATTTTATGGAAGTGGTGGAGTTTCTCAAGAAGCCTGAGAGGTTTACTTCTGTTGGTGCTCGCATACCAAAAGGAGTTCTTCTTGTTGGTCCTCCAGGAACTGGAAAGACTTTGTTAGCCAAGGCTATTGCTGGTGAAGCCGGCGTTCCGTTTTTCTCTATATCAGGTTCTGAGTTTGTTGAGATGTTTGTTGGTATCGGTGCTTCTCGTGTCCGTGATTTGTTCAAGAAGGCTAAAGAGAATGCTCCTTGCATTGTCTTTGTTGATGAAATTGATGCTGTTGGCAGACAAAGAGGGACAGGAATTGGTGGAGGGAATGATGAAAGAGAACAGACCCTTAACCAGCTTTTGACAGAAATGGATGGATTTGAGGGTAATACCGGTGTCATTGTCGTTGCTGCAACTAACAGGGCCGACATTCTTGACTCTGCATTATTAAGACCTGGCCGATTCGATAGACAGGTAGCGGTAGATGTTCCAGATATAAGGGGAAGGACTGAAATCCTAAAGGTTCATGCTAACAATAAAAAGTTTGAAAATGATGTTTCTCTTGAAGTGATTGCAATGAGAACACCTGGTTTCAGTGGAGCTGATCTTGCAAATCTCTTGAATGAAGCTGCTATATTAGCTGGTCGGCGAGGAAGGTCAGGGATTTCATCTAAAGAGATTGACGATTCTATCGATAGGATTGTGGCTGGAATGGAAGGAACATTAATGACAGATGGAAAGAGCAAAAGTTTAGTAGCATATCACGAAGTAGGACATGCCATTTGCGGAACTTTGACTCCTGGTCATGATGCTGTACAAAAGGTAACATTAATTCCTCGCGGTCAAGCTCGGGGTCTTACATGGTTCATTCCTTCCGATGATCCAACCCTGATCTCAAAACAGCAACTCTTTGCAAGAATTGTTGGAGGGTTAGGTGGTAGGGCTGCAGAAGAAGTTATTTTCGGTGAGCCCGAGGTTACAACTGGCGCAGGTGGTGATTTGCAGCAAATCACCGGTATAGCAAGACAGATGGTAGTCACATTTGGAATGTCAGATATTGGTCCTTGGTCACTTATGGACTCATCAGCGCAAAGTGGCGATGTTATCATGAGAATGATGGCAAGGAACTCAATGTCAGAAAAGCTTGCAGAAGACATTGATACTGCTGTCAAGAGATTATCAGATGAAGCATATGAAATTGCCTTGGCACAGATAAGGAGCAACCGTGAGGCAATTGATAAGATTGTGGAAGTCCTTCTGGAAAAGGAGACATTGAGTGGAGATGAATTCCGCGCTCTACTATCGGAGTTTGTTGAAATTCCGGCTGAAAATCGGGTTACTCCTGCTACTCCATTGCCAGTCCCTGCTTGAATGGCGGCCTGTATGTAGTTTCTGTAATATTTATTATGTAATATCCCAGAATATGGAATTACCCTCTACAGCATGCTTCAGATTTGTGAATGACAAGCTTCTAGCTTACTTGTTAATTTTTAGTGTTGATAGTGACAACATACAATATATACAAATAGCAAATATTTGAGTGCAGGAACCATAGTTTGTTTCAATACATCTATTATACTAG
TARGET=144,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03121 Ps_contig_mira-and-tgicl-ass_franssen_28717
SEQUENCE=GTCGGGGACACTCATCTTCACACTCACTCACTCTCACTTTACAATTTACAAACCAAATTCAAAAAAATACAAAACACATAACCAATGGACATACTATCTAATTCCGAGTTCATTGAGTTGATTCCCGGATTACCGAGTGAACTCGGACTAGAATGTCTCACTCGTTTACCTCACTCAGCACACCGAGTCGCACTCCGTGTCTGCAACCAGTGGCGACACTTGTTTCAGAGTAATGACTTTTACTGTCACAGGAAAAAAACCGGTCACACCAGAAAGGTTGCTTGTTTGGTTCAAGCCCGTGAACAGCCGCTTCACGCTGAAGTGGAAAAACCAACTGGGTCAACGCCACCGAGTTACGATATCACTGTGTTTGACCCGGAGAGTATGTCTTGGGATCGGGTTGACCCGGTTCCAGAGTACCCGCTCGGGTTGCCATTGTTCTGTCAACTAATCAGCTGCGAAGGGAAGCTCGTGGTTATGGGTGGATGGAACCCAGCGAGTTACGAACCTTTGACGGCGGTGTTCGTTTATGATTTCCGAACGAACATGTGGAGGCGAGGGAAGGATATGCCGGAGAAGCGTTCGTTTTTTGCTGCTGGATCGGGTCAGGGTCGGGTTTTTGTTGCCGGTGGACACGATGAGAATAAGAACGCCTTGAGTACTGCTTGGACTTATGACCCGAATAGCGACGAATGGACTGTGTTGGCTCCGATGAGTCAGGAACGAGACGAGTGTGAAGGAGTGGTTGTAGGCGGCGAGTTCTGGGTGGTCAGCGGCTACTCTACGGAGAGTCAAGGAATGTTTGATCAGTCGGCGGAAGTGTTGAACACTGCGTCGGGTCAGTGGAGGAAAGTTGAAGGTGTATGGGAAGCGGGTCGGTGCCCCAGATTCTGTGTAGATATCCGGGAAAATGGGAAAGTTGTGGATCCGGGGCTCCGAATTGGGGTTTGTAGTGTTAGGGTCGGGTTGAAGAGCTTGGTGACTGGGTCCGAATACGAAGGAGCGCCGTATGGGTTTTATTTGGTAGAAAATGACGATGGGCAAAACCGTAAATTGACTAAGATAAGTAACGTTCCTCATGGTTATTCTGGGTTTGTTCAATCAGGATGCTGTGTTGAAATCTAGTATAAACCGTTAGATTGAAAAAGGAAAAGTATCTCTCTTAATCTATTTTAATATTTAATATTTTAAGGGGAAGTATATAATCTAGTGTTGTGTTGAGCTGAAAATTGGCATTGTAAATTTGTAATCTGAAAGCTGAATTTTCAAAGCTTGATCAATCATTCACTTGGCAAAATCCATGTATGGTTTCGCAGTGAACTTTCTAGTATAATCTATCACAATTTTACGTTGCTTCAGCAAAATCATGGTACTGCAGTGATTTTGTGAAGTTTTCTATAAATCATCATTTGTTGCTCAATCTATGTTTGCGGAATATTATTTAAAC
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.4069
PRIMER_LEFT_PENALTY=2.903197
PRIMER_RIGHT_PENALTY=0.503696
PRIMER_LEFT_SEQUENCE=GTCGGGGACACTCATCTTCAC
PRIMER_RIGHT_SEQUENCE=CGAGTGAGACATTCTAGTCCGA
PRIMER_LEFT=0,21
PRIMER_RIGHT=163,22
PRIMER_LEFT_TM=61.903
PRIMER_RIGHT_TM=59.496
PRIMER_LEFT_GC_PERCENT=57.143
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=3.4476
PRIMER_LEFT_1_PENALTY=2.903197
PRIMER_RIGHT_1_PENALTY=0.544368
PRIMER_LEFT_1_SEQUENCE=GTCGGGGACACTCATCTTCAC
PRIMER_RIGHT_1_SEQUENCE=CTTGAACCAAACAAGCAACCTT
PRIMER_LEFT_1=0,21
PRIMER_RIGHT_1=294,22
PRIMER_LEFT_1_TM=61.903
PRIMER_RIGHT_1_TM=60.544
PRIMER_LEFT_1_GC_PERCENT=57.143
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=3.4476
PRIMER_LEFT_2_PENALTY=2.903197
PRIMER_RIGHT_2_PENALTY=0.544368
PRIMER_LEFT_2_SEQUENCE=GTCGGGGACACTCATCTTCAC
PRIMER_RIGHT_2_SEQUENCE=AACCAAACAAGCAACCTTTCTG
PRIMER_LEFT_2=0,21
PRIMER_RIGHT_2=290,22
PRIMER_LEFT_2_TM=61.903
PRIMER_RIGHT_2_TM=60.544
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=3.5393
PRIMER_LEFT_3_PENALTY=3.082750
PRIMER_RIGHT_3_PENALTY=0.456523
PRIMER_LEFT_3_SEQUENCE=GTCGGGGACACTCATCTTCA
PRIMER_RIGHT_3_SEQUENCE=GCTGAGTGAGGTAAACGAGTGA
PRIMER_LEFT_3=0,20
PRIMER_RIGHT_3=178,22
PRIMER_LEFT_3_TM=61.083
PRIMER_RIGHT_3_TM=59.543
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=179
PRIMER_PAIR_PENALTY_4=3.5693
PRIMER_LEFT_4_PENALTY=3.082750
PRIMER_RIGHT_4_PENALTY=0.486593
PRIMER_LEFT_4_SEQUENCE=GTCGGGGACACTCATCTTCA
PRIMER_RIGHT_4_SEQUENCE=TGTGCTGAGTGAGGTAAACGAG
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=181,22
PRIMER_LEFT_4_TM=61.083
PRIMER_RIGHT_4_TM=60.487
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_03122 Ps_contig_mira-and-tgicl-ass_franssen_28743
SEQUENCE=ACTAACACAAAAGAGTAAATTGCCAGAAGCCCTNACNAATCNACNACCACAAGTCTTATCCTTTCTAGTTCAACCACATCTCTAAAACACTTGCACTTGTTTGGGAACACCAAACACAAAAACATGGCAACCCTTCAATTTACACCCTTAACTCTTTTCAAACAGACAGCTAAGTTTCCATCATCTTCTTCTTCTTCTTCTTCTTCCCAGTTCAACACAAATAAGAAATCATCATCACTCAGATTCACTGTGAAGTCTTCTTATAAAGTGGTGATTGAGCATGATGGCAAGTCCACACAGCTTGAAGTTGAACCTGATGAGACCATACTGTCAAAGGCATTGGATTCTGGTTTGTCTGTTCCTCATGATTGTAAGCTTGGAGTTTGCATGACTTGCCCTGCTCGTCTTATCAGTGGCAAAGTTGATCAGAGTGATGGAATGCTCAGTGATGATGTTGTGGAACGTGGTTATGCACTTCTGTGTGCCTCTTACCCTCGTTCTGATTGTCATATTAGGGTTATACCAGAGGATGAACTACTCTCAATGCAGTTAGCTACAGCTAATGACTAATCTTGTTTTCAGTTTTTAATGTAATTGCCAGAAATCAATCAATGGAATTGTAGTTTTTTCCTTGTTTCTTATAATAATTGATATTGTCTATCTCTCTGATATGCTAAGTTGCTTTGATTATGTAGTTTTTCATTGATGCATTTGAAATACAACATTTCTAGCATTGAGAGACTCTCTGAACTTTTGAGAGACTATGTGTAAGAAGATTGAGTTAGCTTAGTTTGAAACACTCTGTGTAAGAAACTCTTAAGACTCTGTGTAAGAAGTTGAACCTGAAGTTAGCTAAGTTTGGCTTACACAGCTGAAGTTAGCTTCATTTGAAACGTTACGTTTTAAAATTTTTTTTAAAAAAAAGG
TARGET=184,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1158
PRIMER_LEFT_PENALTY=0.115769
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=ATGGCAACCCTTCAATTTACAC
PRIMER_RIGHT_SEQUENCE=CATCATGCTCAATCACCACTTT
PRIMER_LEFT=123,22
PRIMER_RIGHT=285,22
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.1158
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=ACATGGCAACCCTTCAATTTAC
PRIMER_RIGHT_1_SEQUENCE=CATCATGCTCAATCACCACTTT
PRIMER_LEFT_1=121,22
PRIMER_RIGHT_1=285,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=0.1201
PRIMER_LEFT_2_PENALTY=0.115769
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=ATGGCAACCCTTCAATTTACAC
PRIMER_RIGHT_2_SEQUENCE=AGAATCCAATGCCTTTGACAGT
PRIMER_LEFT_2=123,22
PRIMER_RIGHT_2=347,22
PRIMER_LEFT_2_TM=60.116
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=0.1201
PRIMER_LEFT_3_PENALTY=0.115769
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=ACATGGCAACCCTTCAATTTAC
PRIMER_RIGHT_3_SEQUENCE=AGAATCCAATGCCTTTGACAGT
PRIMER_LEFT_3=121,22
PRIMER_RIGHT_3=347,22
PRIMER_LEFT_3_TM=60.116
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.1626
PRIMER_LEFT_4_PENALTY=0.115769
PRIMER_RIGHT_4_PENALTY=0.046801
PRIMER_LEFT_4_SEQUENCE=ATGGCAACCCTTCAATTTACAC
PRIMER_RIGHT_4_SEQUENCE=TCAACTTCAAGCTGTGTGGACT
PRIMER_LEFT_4=123,22
PRIMER_RIGHT_4=310,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=59.953
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_03123 Ps_contig_mira-and-tgicl-ass_franssen_28744
SEQUENCE=GTATAGACAATNNCATTATTATAAGAAACAAGAAAAACTACAATTCCATTGATTGATTTCTTGGCAATTACATTAAAACTAGAAACAAAGATTAGTCATTAGCTGTAGCTAACTGCATTGAGAGTAGTTCATCCTCTGGTATAACCCTGATATGACAATCAGAACGAGGGTAAGAGGCACACAGAAGTGCATAACCACCGTTCCACAACATCATCACTGAGCATTCCATCACTCTGATCAACTTTGCCACTGATAAGACGAGCAGGGCAAGTCATGCAAACTCCAAGCTTACAATCATGAGGAACAGACAAACCAGAATCCAATGCCTTTGACAGTATGGTCTCATCAGGTTCAACTTCAAGCTGTGTGGACTTGCCATCATGCTCAATCACCACTTTATAAGAAGACTTCACAGTGAATCTGAGTGATGATGATTTCTTATATGTGTTGAACTGAGAAGAAGAAGAAGAAGAAGAAGATGGAAACTTAGTTGTCTGTTTGAAAAGAGTTAAGGGTGTAAACTGAAGGGTTGCCATGTTTTTGTGTTTGGTGTTCCAAAACAAGTGCAAGTCGTTTTTGAGATGTGGTTACCCCC
TARGET=456,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2824
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.282356
PRIMER_LEFT_SEQUENCE=CATCATGCTCAATCACCACTTT
PRIMER_RIGHT_SEQUENCE=ACATGGCAACCCTTCAGTTTAC
PRIMER_LEFT=376,22
PRIMER_RIGHT=537,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.282
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=162
PRIMER_PAIR_PENALTY_1=0.2867
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.282356
PRIMER_LEFT_1_SEQUENCE=AGAATCCAATGCCTTTGACAGT
PRIMER_RIGHT_1_SEQUENCE=ACATGGCAACCCTTCAGTTTAC
PRIMER_LEFT_1=314,22
PRIMER_RIGHT_1=537,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=60.282
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.3292
PRIMER_LEFT_2_PENALTY=0.046801
PRIMER_RIGHT_2_PENALTY=0.282356
PRIMER_LEFT_2_SEQUENCE=TCAACTTCAAGCTGTGTGGACT
PRIMER_RIGHT_2_SEQUENCE=ACATGGCAACCCTTCAGTTTAC
PRIMER_LEFT_2=351,22
PRIMER_RIGHT_2=537,22
PRIMER_LEFT_2_TM=59.953
PRIMER_RIGHT_2_TM=60.282
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=0.3851
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.385098
PRIMER_LEFT_3_SEQUENCE=CATCATGCTCAATCACCACTTT
PRIMER_RIGHT_3_SEQUENCE=GGGGTAACCACATCTCAAAAAC
PRIMER_LEFT_3=376,22
PRIMER_RIGHT_3=593,22
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=59.615
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.4309
PRIMER_LEFT_4_PENALTY=0.148509
PRIMER_RIGHT_4_PENALTY=0.282356
PRIMER_LEFT_4_SEQUENCE=CATGAGGAACAGACAAACCAGA
PRIMER_RIGHT_4_SEQUENCE=ACATGGCAACCCTTCAGTTTAC
PRIMER_LEFT_4=295,22
PRIMER_RIGHT_4=537,22
PRIMER_LEFT_4_TM=60.149
PRIMER_RIGHT_4_TM=60.282
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_03124 Ps_contig_mira-and-tgicl-ass_franssen_28746
SEQUENCE=GAGGTAACTTCCGGGAGGGGGTTTTCCGTAAGGTTTTTTTTCCTAATTTTCCTTGTAACCTTTTTCCTAACGGCCCCCACCTTAACCCACCCTAAAAACCCTTTACCACCAAAACCCAGAGGTTAAAGAGGCAGCGCCTTTTTGCTGTCCGCCTTGACGCCATCTTCGTCTTGGCCCTCAATTTTGCTCTATCAAAAACCCTAGAAAAAAACTCAATTCTCATCAATGGCACCAAAACCAACCAAAGCCGAGAAGAAGCAATCCTACGATGCCAAGCTATGCCAGCTTCTAGACGAGTTTTCTCAGATTCTCATTGTTAACGCTGACAATGTTGGATCTAAGCAGCTTCAGAATATTCGAAGCGGTCTACGTGGGGATTCTGTAGTTCTTATGGGGAAGAACACTATGATGAAGAGATCTGTTAGGATGCATGCTGAGAAGACTGGGAACAATGCTTTTATCAACCTGATTCCTCTCCTTGTTGGAAACGTTGGTTTGATTTTCACCAAAGGAGATTTGAAAGAGGTTAGCGAGGAAGTTGCCAAGTACAAGGTTGGAGCTCCTGCTCGTGTTGGTTTGGTTGCTCCCATTGATGTTGTTGTTCCACCAGGCAACACAGGGCTCGACCCATCCCAAACCTCTTTCTTCCAGGTGCTCAACATTCCCACAAAGATTAACAAGGGTACTGTTGAAATCATTACACCTGTTGAACTAATCAAGAAGGGAGACAAGGTTGGATCTTCTGAAGCTGCATTGCTTGCCAAGTTAGGCATTAGGCCCTTTTCATATGGTCTGGTGGTCCTTTCTGTCTATGACAATGGCTCCGTGTTTAGCCCTGAGGTTTTGGATCTTACTGATGATGACCTTGTTGCAAAGTTTGCTATTGGTGTCTCAATGGTTACCTCACTTTCATTGGCCATCTCATACCCAACATTGGCAGCTGCTCCACACATGTTTGTGAATGCCTACAAGAATGTTCTTGCCATTGCAGTGGCAACAGAATATTCCTACCCAGAGGCTGACGAGGTTAAGGAATATCTCAAGGATCCAAGTAAGTTTGCTGTAGCTGCTGCTGCTGCTCCTGCCAATATAACTTATGATGCACCTGCTGCTGCCAAGGAGGAGAAGAAGGAGGAGCCTGAAGAAGAATCTGATGAAGACATTGGTTTTGGTCTTTTTGATGAGTAAACCCTTTAAATTTATGTGTAATTCTTATTTTGGTTTTGTTAGATTTTCTGAGACATTTCTTCATTTTAGAAGTTATGTAGTTTGCTTATTTTGAGGTTTGTATGTTACATTATGACTTCTTTGATTATGTTATATTTAAGTTTTGCTTATAATCTCTGTATGGCAATTGAACTGATAATCGTCTCTTCTCTTTTGAAGTGAAAATTAAAATCTTACTATTATTTTATCAAGTT
TARGET=1066,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1441
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.140508
PRIMER_LEFT_SEQUENCE=TTTGCTATTGGTGTCTCAATGG
PRIMER_RIGHT_SEQUENCE=ATTCTTCTTCAGGCTCCTCCTT
PRIMER_LEFT=876,22
PRIMER_RIGHT=1149,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.859
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.1807
PRIMER_LEFT_1_PENALTY=0.040146
PRIMER_RIGHT_1_PENALTY=0.140508
PRIMER_LEFT_1_SEQUENCE=CACTTTCATTGGCCATCTCATA
PRIMER_RIGHT_1_SEQUENCE=ATTCTTCTTCAGGCTCCTCCTT
PRIMER_LEFT_1=904,22
PRIMER_RIGHT_1=1149,22
PRIMER_LEFT_1_TM=59.960
PRIMER_RIGHT_1_TM=59.859
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=0.3403
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.336717
PRIMER_LEFT_2_SEQUENCE=TTTGCTATTGGTGTCTCAATGG
PRIMER_RIGHT_2_SEQUENCE=GATTCTTCTTCAGGCTCCTCCT
PRIMER_LEFT_2=876,22
PRIMER_RIGHT_2=1150,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=60.337
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.3403
PRIMER_LEFT_3_PENALTY=0.003589
PRIMER_RIGHT_3_PENALTY=0.336717
PRIMER_LEFT_3_SEQUENCE=TTTGCTATTGGTGTCTCAATGG
PRIMER_RIGHT_3_SEQUENCE=AGATTCTTCTTCAGGCTCCTCC
PRIMER_LEFT_3=876,22
PRIMER_RIGHT_3=1151,22
PRIMER_LEFT_3_TM=59.996
PRIMER_RIGHT_3_TM=60.337
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.3445
PRIMER_LEFT_4_PENALTY=0.203971
PRIMER_RIGHT_4_PENALTY=0.140508
PRIMER_LEFT_4_SEQUENCE=GGCAACAGAATATTCCTACCCA
PRIMER_RIGHT_4_SEQUENCE=ATTCTTCTTCAGGCTCCTCCTT
PRIMER_LEFT_4=992,22
PRIMER_RIGHT_4=1149,22
PRIMER_LEFT_4_TM=60.204
PRIMER_RIGHT_4_TM=59.859
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_03125 Ps_contig_mira-and-tgicl-ass_franssen_28758
SEQUENCE=TTGTAGTCCAACGACATTAACTAAAGAATTGTTAGTGTTTCCTATTCGAGTATTGACACTGAGGATTATTGTAAACTAGAACCCAAACCAATAATCAAACAAAACCAAGTGTTAATAGCTGAATCCAAATGNGNATCAGGGAAAAGGAACACTGTGGTGCTGCAGACAAGTAAACTCTCTTTTGTTGCACTAAAACCATTTAGTGCTCTTGCAAGTTCATCAAAATGTTCACCACCAGCTTCAATATCAAAATCAATTCCATCCAAAACAGCGTCACCTAATGGCCTTGAAGTTGACGTGCCACCCAAAGAAATTGTTCCATAGATAGTTTGCAACTTGTGTAGCATCATCAGCGGAGCTAAGAGAGTAGCTTCCGGCGCCNCCGAGAGAGAGAAGAACTTTGATTCCTTTYGCTTGACATGCTTGAATCTCGGAACTAAACTTTGTGCATTCATTGGCACTTGGATTACAATGGCCAGCAAGGTTTAGTTGCGGGGTTTGGCCATTGCCGAAAGTGGACAGAAAAGCTATGTTTACAAATTGGTAGTTGTTTGTGTTGCAAGCATCTGCCAAGGAACCTTCACCTCCATTTTGGCCCCAATAGACTGCAATTCCAGCAGCATGAGAATATTTGAATAGGGAAACTAGGAAAAGAGGGAACAATAGGATTGAAAGTGGTTTTAAGGATGCCATTGTTTTTTAAGGTTGTTTTTGAGTTGTCGTTCC
TARGET=385,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03126 Ps_contig_mira-and-tgicl-ass_franssen_28759
SEQUENCE=ATACTTCTATATTTTATTCTATTATAAATTAGTTAAAGTTGTCATCATTTGTGNCGNNGNYNANTNAGACAACTCAAAAAACAACCTTAAAAAACATGGCATCCTTAAAACAACTTTCAATCCTATTTTTCCCTCTTTTCCTAGTTTCCCTATTCAGATACTCTCATGCTGCTGGAATTGCAGTCTATTGGGGCCAAAATGGAGGTGAAGGTTCCTTGGCAGATGCTTGCAACACAAACAACTACCAATTTGTAAACATAGCTTTTCTCTCCACTTTCGGCAATGGCCAAACCCCGCAACTAAACCTTGCTGGCCATTGTAATCCAAGTGCCAATGAATGCACAAAGTTTAGTTCCGAGATTCAAGCATGTCAAGCGAAAGGAATCAAAGTTCTTCTCTCTCTCGGAGGCGGCGCCGGAAGCTACTCTCTTAGCTCCGCTGATGATGCTACACAAGTTGCAAACTATCTATGGAACAATTTCTTGGGTGGCACGTCAACTTCAAGGCCATTAGGTGACGCTGTTTTGGATGGAATTGATTTTGATATTGAAGCTGGTGGTCAACATTTTGATGAACTTGCAAGAGCACTAAATGGTTTTAGTGCACAAAAGAGAGTTTACTTGTCTGCAGCACCACAGTGTCCTTTCCCTGATGCTCATTTGGATTCAGCTATTAACACTGGTTTGTTTGATTACGTTTGGGTTCAGTTTTACAATAATCCTCAGTGTCAATACTCGAATGGAAACACTAACAATCTTGTTAATGCTTGGACTCAATGGACTTCAAGTCAAGCTAAACAAGTGTTCTTAGGTGTTCCTGCTAATGCTGCTGCTGCTCCAAGTGGTGGCTTTATTCCTTCTGATGTTTTGATTTCTCAAGTTCTTCCTGCTATTAAGAGTTCTCCTAAATATGGTGGAGTTATGATTTGGGATAGATTCAATGATGGACAAAGTGGATACAGTAATGCTATTAAGGGCAGTGTTTAATCAGTTTAATTTCTGGTTAAATTTATAGTCTTTGTTATGTATTTGAAATAATTAGTAGCATGTTTAGTGGGTTTTGTTCTACTCATTAATAAGTGCTACGTGATCACTTGTTATACATGTAATAATAACGGGACCTTTCAAATAATGTATGATTTCCAATAAATATGCAGAAACTTATCATCAAGTACTTATACANTTGGCTTGTGCAACTTTAAA
TARGET=395,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03127 Ps_contig_mira-and-tgicl-ass_franssen_28759
SEQUENCE=ATACTTCTATATTTTATTCTATTATAAATTAGTTAAAGTTGTCATCATTTGTGNCGNNGNYNANTNAGACAACTCAAAAAACAACCTTAAAAAACATGGCATCCTTAAAACAACTTTCAATCCTATTTTTCCCTCTTTTCCTAGTTTCCCTATTCAGATACTCTCATGCTGCTGGAATTGCAGTCTATTGGGGCCAAAATGGAGGTGAAGGTTCCTTGGCAGATGCTTGCAACACAAACAACTACCAATTTGTAAACATAGCTTTTCTCTCCACTTTCGGCAATGGCCAAACCCCGCAACTAAACCTTGCTGGCCATTGTAATCCAAGTGCCAATGAATGCACAAAGTTTAGTTCCGAGATTCAAGCATGTCAAGCGAAAGGAATCAAAGTTCTTCTCTCTCTCGGAGGCGGCGCCGGAAGCTACTCTCTTAGCTCCGCTGATGATGCTACACAAGTTGCAAACTATCTATGGAACAATTTCTTGGGTGGCACGTCAACTTCAAGGCCATTAGGTGACGCTGTTTTGGATGGAATTGATTTTGATATTGAAGCTGGTGGTCAACATTTTGATGAACTTGCAAGAGCACTAAATGGTTTTAGTGCACAAAAGAGAGTTTACTTGTCTGCAGCACCACAGTGTCCTTTCCCTGATGCTCATTTGGATTCAGCTATTAACACTGGTTTGTTTGATTACGTTTGGGTTCAGTTTTACAATAATCCTCAGTGTCAATACTCGAATGGAAACACTAACAATCTTGTTAATGCTTGGACTCAATGGACTTCAAGTCAAGCTAAACAAGTGTTCTTAGGTGTTCCTGCTAATGCTGCTGCTGCTCCAAGTGGTGGCTTTATTCCTTCTGATGTTTTGATTTCTCAAGTTCTTCCTGCTATTAAGAGTTCTCCTAAATATGGTGGAGTTATGATTTGGGATAGATTCAATGATGGACAAAGTGGATACAGTAATGCTATTAAGGGCAGTGTTTAATCAGTTTAATTTCTGGTTAAATTTATAGTCTTTGTTATGTATTTGAAATAATTAGTAGCATGTTTAGTGGGTTTTGTTCTACTCATTAATAAGTGCTACGTGATCACTTGTTATACATGTAATAATAACGGGACCTTTCAAATAATGTATGATTTCCAATAAATATGCAGAAACTTATCATCAAGTACTTATACANTTGGCTTGTGCAACTTTAAA
TARGET=824,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03128 Ps_contig_mira-and-tgicl-ass_franssen_28770
SEQUENCE=CGGACAGTGGTAGTTCAACGACAGAGTACGCGGGGAGGTAACAGTACTCAAATAATGAGCAAGTTAAGAATCCCAATACTACTAGTACTATTTATAGTCTCCTGCTGCTCAGCAGAGCAATGCGGAACACAAGCTGGGGGTGCTTTGTGCCCCGGTGGATTATGCTGCAGCAAATTCGGTTGGTGCGGTTCAACCTCCGAATACTGCGGTGATGGTTGCCAGAGTCAGTGTAGTGGAAGCAGCGGCGGCGGCACCCTTTCTAGCCTCATCTCCGGTGACACCTTCAACAACATGCTCAAACATCGCAATGATAATGCTTGTCAAGGGAAAGGCTTTTACACCTACGACGCTTTCCTTTCAGCTGCAAAGGCTTTCCCCAACTTCGCCAATAAAGGAGATACCGCCACTAAAAAAAGAGAAATCGCTGCTTTCTTAGGTCAAACTTCTCACGAAACCACCGGTGGATGGCCAACTGCACCCGATGGTCCCTACGCTTGGGGATACTGCTTTCTCAGAGAACAGAATCCCAGCACCTACTGCCAAGCAAGCTCTGAATTTCCATGTGCTTCTGGAAAACAATACTACGGTAGAGGTCCCATTCAGATCTCATGGAATTACAACTACGGACAGTGTGGAAGAGCGATTGGTGTTGACTTGCTCAATAATCCAGACCTCGTCGCTACTGATCCTGTTATATCCTTCAAGACCGCTTTATGGTTCTGGATGACGCCTCAGTCACCTAAACCATCCTGCCACGACGTTATCACCGGAGGGTGGACTCCGTCGAGTGCTGATCGTGCGGCGGGGAGGCTTCCCGGATACGGAACAGTGACGAACATCATAAACGGAGGACTTGAATGCGGGAGAGGACAGGATAGTAGAGTGCAGGATCGGATTGGATTTTACAAGAGATACTGTGACATATTTGGAATTGGATACGGAGATAATCTTGACTGTTACTCTCAGAGGCCATTCGGATCTTCACTCTCGTTGTCATCAATCCTTCTCGACACCATCGCAGCAGCCTAGATTGGCTTTTTCTTCAAAAATAAATAAATGTGAATCACGGTTCACCTACTTCGGTTGACTCATCTATATATGAGTGTGACACATGTATAATATACTACTACTACTATCAATGGATTTCAATCCTTACTCGACTCACGCATACTTTTCAAATCAAATAACACTGTGCTTATCTTTTAGTAGTATATTGTTTTTCTCAAAGAYACATTTCATTCTCGACTATCATTATCACCACTATTGTCATCT
TARGET=1047,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03129 Ps_contig_mira-and-tgicl-ass_franssen_28770
SEQUENCE=CGGACAGTGGTAGTTCAACGACAGAGTACGCGGGGAGGTAACAGTACTCAAATAATGAGCAAGTTAAGAATCCCAATACTACTAGTACTATTTATAGTCTCCTGCTGCTCAGCAGAGCAATGCGGAACACAAGCTGGGGGTGCTTTGTGCCCCGGTGGATTATGCTGCAGCAAATTCGGTTGGTGCGGTTCAACCTCCGAATACTGCGGTGATGGTTGCCAGAGTCAGTGTAGTGGAAGCAGCGGCGGCGGCACCCTTTCTAGCCTCATCTCCGGTGACACCTTCAACAACATGCTCAAACATCGCAATGATAATGCTTGTCAAGGGAAAGGCTTTTACACCTACGACGCTTTCCTTTCAGCTGCAAAGGCTTTCCCCAACTTCGCCAATAAAGGAGATACCGCCACTAAAAAAAGAGAAATCGCTGCTTTCTTAGGTCAAACTTCTCACGAAACCACCGGTGGATGGCCAACTGCACCCGATGGTCCCTACGCTTGGGGATACTGCTTTCTCAGAGAACAGAATCCCAGCACCTACTGCCAAGCAAGCTCTGAATTTCCATGTGCTTCTGGAAAACAATACTACGGTAGAGGTCCCATTCAGATCTCATGGAATTACAACTACGGACAGTGTGGAAGAGCGATTGGTGTTGACTTGCTCAATAATCCAGACCTCGTCGCTACTGATCCTGTTATATCCTTCAAGACCGCTTTATGGTTCTGGATGACGCCTCAGTCACCTAAACCATCCTGCCACGACGTTATCACCGGAGGGTGGACTCCGTCGAGTGCTGATCGTGCGGCGGGGAGGCTTCCCGGATACGGAACAGTGACGAACATCATAAACGGAGGACTTGAATGCGGGAGAGGACAGGATAGTAGAGTGCAGGATCGGATTGGATTTTACAAGAGATACTGTGACATATTTGGAATTGGATACGGAGATAATCTTGACTGTTACTCTCAGAGGCCATTCGGATCTTCACTCTCGTTGTCATCAATCCTTCTCGACACCATCGCAGCAGCCTAGATTGGCTTTTTCTTCAAAAATAAATAAATGTGAATCACGGTTCACCTACTTCGGTTGACTCATCTATATATGAGTGTGACACATGTATAATATACTACTACTACTATCAATGGATTTCAATCCTTACTCGACTCACGCATACTTTTCAAATCAAATAACACTGTGCTTATCTTTTAGTAGTATATTGTTTTTCTCAAAGAYACATTTCATTCTCGACTATCATTATCACCACTATTGTCATCT
TARGET=1122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03130 Ps_contig_mira-and-tgicl-ass_franssen_28772
SEQUENCE=GTAGGTGAACCGTGATTCACATTTATTTATTTTTGTAAGAAAAAGCCAATCTAGGCTGCTGCGATGGTGTCGAGAAGGATTGATGACAACGAGAGTGAAGATCCGAATGGCCTCTGAGAGTAACAGTCAAGATTATCTCCGTATCCAATTCCAAATATGTCACAGTATCTCTTGTAAAATCCAATCCGATCCTGCACTCTACTATCCTGTCCTCTCCCGCATTCAAGTCCTCCGTTTATGATGTTCGTCACTGTTCCGTATCCGGGAAGCCCTCCCCGCGCACGATCAGCACTCGACGGATCCCACCCTCGGTNATACGTCGTGGCAGGATTGGTTTAGGNGACTGGAGGCTCNTCCAGACCNTAGCGT
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03131 Ps_contig_mira-and-tgicl-ass_franssen_28773
SEQUENCE=ACAATAACCATAGTGTTGGGATTCTCACTATTGTATGTTACCAATAAGAAGAAAATTTTAAGCGACCACACCATAAGCACAACATTTACCATAGGTCTAACATTAAAGCTGCATAAAAGAAAAGACAAAAACAAGCTTACATGCATATGAACGCAGTTCATGTCTAGCTATGTACTAGTATAATAAAAGGTGTCTTAGGTCCAAACTCTTGAAAGAGCATCAACCTGCAGTGCAGTCTTTGCACACTGACACTATGAAGTTCAGCACAAAAATACACAAGCTAGAATAACCACACACTTGGCCGGTAACAAGTACTGCCCTGCCCTTATTTATTAGTGTATAGACACACGCATATACATATATTATCTTCTAAAGGCAAGTATTAAGGATGGGAGTGGGGCTTCTCCAAGTCCTCAAGGCTGATGGGGCGAAACCAAGCCTTCTCCTCAAGCACTGAGCCTCCTGCAGCAACGGCAGCAGTTGCATCCGCAGTGGAGGCTGAATGGAAGCCAGCAGTGACTTGCTCGCCTGGGGGCCCAAATACACCAGTCTGTGGGTGTGGAGCCCAGTATTTGGTGGATTGAGTTGCCTGGATCACATCATCAGGGACTGGCCCGGAGTGACTGTGATCATCAACGTTCTTGTCGTACAATGAACTGTATGCTGCCCTCCTAGATGCGATTGGTAGAGGAGAAGGTGCAGAGTTGGAGTTCCAGATCTGGTTGAAAACTCGCTTCCCGAAGCCAGTGATCGATCGGCTACTCGAATTGGCTGCCATACAATGCAACAAACAAAGAGAGTAAAAAAGGAGTAGTAGCTGAAATGAAAATGTGATTTGTTGTTGTTGTTGGTCCGTGGTTTGTGAAATGGATATATATTTATACACAAAAATAGGAAGAGTCAAAGGTAGAGACACTTCTATCTTTGAAATAGTGAAATGGAAAGATGCTGTCTACCCCGAC
TARGET=836,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5905
PRIMER_LEFT_PENALTY=0.077340
PRIMER_RIGHT_PENALTY=0.513182
PRIMER_LEFT_SEQUENCE=GAGAAGGTGCAGAGTTGGAGTT
PRIMER_RIGHT_SEQUENCE=GGGTAGACAGCATCTTTCCATT
PRIMER_LEFT=690,22
PRIMER_RIGHT=957,22
PRIMER_LEFT_TM=59.923
PRIMER_RIGHT_TM=59.487
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=0.5905
PRIMER_LEFT_1_PENALTY=0.077340
PRIMER_RIGHT_1_PENALTY=0.513182
PRIMER_LEFT_1_SEQUENCE=AGAAGGTGCAGAGTTGGAGTTC
PRIMER_RIGHT_1_SEQUENCE=GGGTAGACAGCATCTTTCCATT
PRIMER_LEFT_1=691,22
PRIMER_RIGHT_1=957,22
PRIMER_LEFT_1_TM=59.923
PRIMER_RIGHT_1_TM=59.487
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.7416
PRIMER_LEFT_2_PENALTY=0.228464
PRIMER_RIGHT_2_PENALTY=0.513182
PRIMER_LEFT_2_SEQUENCE=AGATGCGATTGGTAGAGGAGAA
PRIMER_RIGHT_2_SEQUENCE=GGGTAGACAGCATCTTTCCATT
PRIMER_LEFT_2=673,22
PRIMER_RIGHT_2=957,22
PRIMER_LEFT_2_TM=60.228
PRIMER_RIGHT_2_TM=59.487
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.7416
PRIMER_LEFT_3_PENALTY=0.228464
PRIMER_RIGHT_3_PENALTY=0.513182
PRIMER_LEFT_3_SEQUENCE=GATGCGATTGGTAGAGGAGAAG
PRIMER_RIGHT_3_SEQUENCE=GGGTAGACAGCATCTTTCCATT
PRIMER_LEFT_3=674,22
PRIMER_RIGHT_3=957,22
PRIMER_LEFT_3_TM=60.228
PRIMER_RIGHT_3_TM=59.487
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.7623
PRIMER_LEFT_4_PENALTY=0.249074
PRIMER_RIGHT_4_PENALTY=0.513182
PRIMER_LEFT_4_SEQUENCE=GATCTGGTTGAAAACTCGCTTC
PRIMER_RIGHT_4_SEQUENCE=GGGTAGACAGCATCTTTCCATT
PRIMER_LEFT_4=715,22
PRIMER_RIGHT_4=957,22
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=59.487
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_03132 Ps_contig_mira-and-tgicl-ass_franssen_28784
SEQUENCE=TTTTCTTATGTTCTTAACTTAATTTCTCATAAGAACATAAAACTGATCTCCCTCCTAACCTCAATGTCCACATTTTTCATGTAAAAAAAACCTAATTATAGCTAGCCGACATCAAATGTTAAGAGAAATGCAAACAAACAAAAAAACTATTATAGCTAGCTGACATCAAATGTTAAGAGAAATACACAAAGGAGTACTTGCTCCAAATTTCATAAGTTGCTGCTTCAAATGTACTTCACATATACAAGCATTTAAAACGACAAGAAAACTCAATAGATAGTAATTTTGTCTACTGAATGTTTAGGCTCTCTTCATTAGTACAACCATTATCACCATAACCGCCAATCCCATGAAACTTCCTCCCATAAAATTTTTATAATCCATGAAATTGGCTCCACCACCATCAGCTGAAGATTTGTCTTTCTCAGCTGTTGGGAATATGTTCTGAAAGAAATTGGACATTCTGCTAACCATTTCAGTAACTGTAAGCTTCAAATTCTGCAGAATAGTCATAAAATCAAAAGCATTTCCCTCCTCTTTTTTAGGAGTTTCTACAGTTTCAGAACCAGCAAAAGCAGCATCATTTGATGACTCTACCGCCGCTAGCTGTGATTCGAAGAAACTAGTGACAGCAGGGTTTTGCATGACAGCATTCCATATATTAGGGTCACAAGCAATAGATTGCACCACAGTCTGGGCCTCAGGACTTGTGCTGAGCAATTGAAAAGCATGAATGGCATGCTTAGGCACTGAAGGACTTGAAATAGCCTCAACAAGACAAGATTTTGTCCCTATTTCCTTAGGAGGAGGAGACACAACAGAGATTTGACTACCAGGAGATGAACCCTCACATTGAGAACTACCAGATGAAAGGTAAATTTGATCAATAGCGTCCTTCAATTCTGCGGTAGCTTCCTGTGCTTCCTTAAAGGTAGGAACATCTCCGAACACCACTCTTGGCGTGGGCTCTCCGCCGGTCATGAACAGATCGCCTTCATCGGCGAATTCCCAGTCAGACAAATCGCCTGACACCGCCGTATGCAGTGGCTTCACCTCAGCGCCTTTCGCTCCCTGCGTTGAAAGTCTAGTTGGCGATGATGTACGAGATGCGTTGCGAACCGGATGCTCGACCGGGTACGTAGCCGGTGTTCCTCTGAAGCCTGACCTAGCGACGCCGATGCCGGCGAGTTTGGATACCGTCCTCATGGATCCTCCACCGCCCATTTCCCAATCAGTCTCGCTGGCCCTACTGCACGCGGAACAAAGTAATAAAATAAAATAAATTCCCCGACTACTCTGCGTTGANTANCACTGCTT
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8296
PRIMER_LEFT_PENALTY=0.771376
PRIMER_RIGHT_PENALTY=0.058242
PRIMER_LEFT_SEQUENCE=GCCGACATCAAATGTTAAGAGA
PRIMER_RIGHT_SEQUENCE=GATTGGCGGTTATGGTGATAAT
PRIMER_LEFT=104,22
PRIMER_RIGHT=346,22
PRIMER_LEFT_TM=59.229
PRIMER_RIGHT_TM=59.942
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.8730
PRIMER_LEFT_1_PENALTY=0.292834
PRIMER_RIGHT_1_PENALTY=0.580184
PRIMER_LEFT_1_SEQUENCE=CTGATCTCCCTCCTAACCTCAA
PRIMER_RIGHT_1_SEQUENCE=TTGGAGCAAGTACTCCTTTGTG
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=59.707
PRIMER_RIGHT_1_TM=59.420
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.9481
PRIMER_LEFT_2_PENALTY=0.771376
PRIMER_RIGHT_2_PENALTY=0.176730
PRIMER_LEFT_2_SEQUENCE=GCCGACATCAAATGTTAAGAGA
PRIMER_RIGHT_2_SEQUENCE=TTTATGGGAGGAAGTTTCATGG
PRIMER_LEFT_2=104,22
PRIMER_RIGHT_2=368,22
PRIMER_LEFT_2_TM=59.229
PRIMER_RIGHT_2_TM=60.177
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=1.0041
PRIMER_LEFT_3_PENALTY=0.423908
PRIMER_RIGHT_3_PENALTY=0.580184
PRIMER_LEFT_3_SEQUENCE=TCTCCCTCCTAACCTCAATGTC
PRIMER_RIGHT_3_SEQUENCE=TTGGAGCAAGTACTCCTTTGTG
PRIMER_LEFT_3=46,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=59.576
PRIMER_RIGHT_3_TM=59.420
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=1.1338
PRIMER_LEFT_4_PENALTY=1.075531
PRIMER_RIGHT_4_PENALTY=0.058242
PRIMER_LEFT_4_SEQUENCE=AGCTAGCCGACATCAAATGTTA
PRIMER_RIGHT_4_SEQUENCE=GATTGGCGGTTATGGTGATAAT
PRIMER_LEFT_4=99,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=58.924
PRIMER_RIGHT_4_TM=59.942
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=5.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_03133 Ps_contig_mira-and-tgicl-ass_franssen_28784
SEQUENCE=TTTTCTTATGTTCTTAACTTAATTTCTCATAAGAACATAAAACTGATCTCCCTCCTAACCTCAATGTCCACATTTTTCATGTAAAAAAAACCTAATTATAGCTAGCCGACATCAAATGTTAAGAGAAATGCAAACAAACAAAAAAACTATTATAGCTAGCTGACATCAAATGTTAAGAGAAATACACAAAGGAGTACTTGCTCCAAATTTCATAAGTTGCTGCTTCAAATGTACTTCACATATACAAGCATTTAAAACGACAAGAAAACTCAATAGATAGTAATTTTGTCTACTGAATGTTTAGGCTCTCTTCATTAGTACAACCATTATCACCATAACCGCCAATCCCATGAAACTTCCTCCCATAAAATTTTTATAATCCATGAAATTGGCTCCACCACCATCAGCTGAAGATTTGTCTTTCTCAGCTGTTGGGAATATGTTCTGAAAGAAATTGGACATTCTGCTAACCATTTCAGTAACTGTAAGCTTCAAATTCTGCAGAATAGTCATAAAATCAAAAGCATTTCCCTCCTCTTTTTTAGGAGTTTCTACAGTTTCAGAACCAGCAAAAGCAGCATCATTTGATGACTCTACCGCCGCTAGCTGTGATTCGAAGAAACTAGTGACAGCAGGGTTTTGCATGACAGCATTCCATATATTAGGGTCACAAGCAATAGATTGCACCACAGTCTGGGCCTCAGGACTTGTGCTGAGCAATTGAAAAGCATGAATGGCATGCTTAGGCACTGAAGGACTTGAAATAGCCTCAACAAGACAAGATTTTGTCCCTATTTCCTTAGGAGGAGGAGACACAACAGAGATTTGACTACCAGGAGATGAACCCTCACATTGAGAACTACCAGATGAAAGGTAAATTTGATCAATAGCGTCCTTCAATTCTGCGGTAGCTTCCTGTGCTTCCTTAAAGGTAGGAACATCTCCGAACACCACTCTTGGCGTGGGCTCTCCGCCGGTCATGAACAGATCGCCTTCATCGGCGAATTCCCAGTCAGACAAATCGCCTGACACCGCCGTATGCAGTGGCTTCACCTCAGCGCCTTTCGCTCCCTGCGTTGAAAGTCTAGTTGGCGATGATGTACGAGATGCGTTGCGAACCGGATGCTCGACCGGGTACGTAGCCGGTGTTCCTCTGAAGCCTGACCTAGCGACGCCGATGCCGGCGAGTTTGGATACCGTCCTCATGGATCCTCCACCGCCCATTTCCCAATCAGTCTCGCTGGCCCTACTGCACGCGGAACAAAGTAATAAAATAAAATAAATTCCCCGACTACTCTGCGTTGANTANCACTGCTT
TARGET=1268,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7289
PRIMER_LEFT_PENALTY=0.117281
PRIMER_RIGHT_PENALTY=1.611571
PRIMER_LEFT_SEQUENCE=TTGAAAGTCTAGTTGGCGATGA
PRIMER_RIGHT_SEQUENCE=TANTCAACGCAGAGTAGTCGGG
PRIMER_LEFT=1076,22
PRIMER_RIGHT=1307,22
PRIMER_LEFT_TM=59.883
PRIMER_RIGHT_TM=61.612
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.50
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=1.8208
PRIMER_LEFT_1_PENALTY=0.209271
PRIMER_RIGHT_1_PENALTY=1.611571
PRIMER_LEFT_1_SEQUENCE=CTAGTTGGCGATGATGTACGAG
PRIMER_RIGHT_1_SEQUENCE=TANTCAACGCAGAGTAGTCGGG
PRIMER_LEFT_1=1084,22
PRIMER_RIGHT_1=1307,22
PRIMER_LEFT_1_TM=59.791
PRIMER_RIGHT_1_TM=61.612
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.50
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=1.9518
PRIMER_LEFT_2_PENALTY=0.340219
PRIMER_RIGHT_2_PENALTY=1.611571
PRIMER_LEFT_2_SEQUENCE=GTCTAGTTGGCGATGATGTACG
PRIMER_RIGHT_2_SEQUENCE=TANTCAACGCAGAGTAGTCGGG
PRIMER_LEFT_2=1082,22
PRIMER_RIGHT_2=1307,22
PRIMER_LEFT_2_TM=59.660
PRIMER_RIGHT_2_TM=61.612
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.50
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=2.2693
PRIMER_LEFT_3_PENALTY=0.657742
PRIMER_RIGHT_3_PENALTY=1.611571
PRIMER_LEFT_3_SEQUENCE=TAGTTGGCGATGATGTACGAGA
PRIMER_RIGHT_3_SEQUENCE=TANTCAACGCAGAGTAGTCGGG
PRIMER_LEFT_3=1085,22
PRIMER_RIGHT_3=1307,22
PRIMER_LEFT_3_TM=60.658
PRIMER_RIGHT_3_TM=61.612
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.50
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=2.2693
PRIMER_LEFT_4_PENALTY=0.657742
PRIMER_RIGHT_4_PENALTY=1.611571
PRIMER_LEFT_4_SEQUENCE=TCTAGTTGGCGATGATGTACGA
PRIMER_RIGHT_4_SEQUENCE=TANTCAACGCAGAGTAGTCGGG
PRIMER_LEFT_4=1083,22
PRIMER_RIGHT_4=1307,22
PRIMER_LEFT_4_TM=60.658
PRIMER_RIGHT_4_TM=61.612
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.50
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_03134 Ps_contig_mira-and-tgicl-ass_franssen_28792
SEQUENCE=TCGGGGGTTGATTGATTAGGTTTAGAATAAGAGAGGGACCACCGGACCCATAAAGTCTCCTAATCCTAACTCTTTCTTCTTCTTCAACTCCTCGAACGTTTCACACACACAAGAAGAAAAGAGAAATCATCGACCATGTCCGACGAAGAGCACCATTTCGAAGCTGCCGCCGACGCCGGAGCATCCAAAACCTATCCTCAGCAGGCCGGTACCATTCGCAAGAACGGTTACATCGTCATCAAAGGCAGGCCATGCAAGGTTGTTGAAGTTTCTACTTCAAAAACAGGAAAGCATGGACATGCAAAGTGTCACTTTGTTGCAATTGATATTTTCAATGGGAAAAAGCTTGAAGATATTGTTCCTTCATCTCACAATTGTGATGTTCCCCATGTGAACCGTACCGACTATCAGCTGATTGATATATCTGAAGATGGTTTTGTGAGTCTGTTGACTGATAACGGAAGTACCAAGGATGATTTGAAACTTCCCACTGATGATGCACTCCTTACTCAGATTAAAGATGGATTTGCTGAAGGAAAAGATCTTGTGGTTTCTGTCATGTCTGCAATGGGAGAAGAGCAGATATGTTCTTTGAAGGACATTGGTCCTAAGAATTAGAGTCATATTCTCTGAACTGGCTATGGCTATGGCTATGGGTGATAGCAAGTATTTTATTCTTATGTTGCTAACAGGTAGTAATTTTTCATACGCATGTGTGCGCAATAAAATTGAAAAGGATGGAAGCTGCCTTGAGCTTGAGGCGAGAAATAGGAACTTTATTTATCTTTTGTCTCTTAAGTTATTAAACCCTGCAGTTTTGGCTGGTTGAATTTGTCATAAATGTACCTTATAACTTTATGCTTTTGTTGAGTTTAATATTGAAACCTTATCACTCTTCTCTAGAAAACC
TARGET=74,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5231
PRIMER_LEFT_PENALTY=0.390220
PRIMER_RIGHT_PENALTY=0.132857
PRIMER_LEFT_SEQUENCE=TAGAATAAGAGAGGGACCACCG
PRIMER_RIGHT_SEQUENCE=TTTGATGACGATGTAACCGTTC
PRIMER_LEFT=22,22
PRIMER_RIGHT=242,22
PRIMER_LEFT_TM=59.610
PRIMER_RIGHT_TM=59.867
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.7530
PRIMER_LEFT_1_PENALTY=0.390220
PRIMER_RIGHT_1_PENALTY=0.362762
PRIMER_LEFT_1_SEQUENCE=TAGAATAAGAGAGGGACCACCG
PRIMER_RIGHT_1_SEQUENCE=CTGCCTTTGATGACGATGTAAC
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=247,22
PRIMER_LEFT_1_TM=59.610
PRIMER_RIGHT_1_TM=59.637
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.7753
PRIMER_LEFT_2_PENALTY=0.390220
PRIMER_RIGHT_2_PENALTY=0.385098
PRIMER_LEFT_2_SEQUENCE=TAGAATAAGAGAGGGACCACCG
PRIMER_RIGHT_2_SEQUENCE=CTGAGGATAGGTTTTGGATGCT
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=200,22
PRIMER_LEFT_2_TM=59.610
PRIMER_RIGHT_2_TM=59.615
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.7753
PRIMER_LEFT_3_PENALTY=0.390220
PRIMER_RIGHT_3_PENALTY=0.385098
PRIMER_LEFT_3_SEQUENCE=TAGAATAAGAGAGGGACCACCG
PRIMER_RIGHT_3_SEQUENCE=CTGCTGAGGATAGGTTTTGGAT
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=203,22
PRIMER_LEFT_3_TM=59.610
PRIMER_RIGHT_3_TM=59.615
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=0.8414
PRIMER_LEFT_4_PENALTY=0.390220
PRIMER_RIGHT_4_PENALTY=0.451213
PRIMER_LEFT_4_SEQUENCE=TAGAATAAGAGAGGGACCACCG
PRIMER_RIGHT_4_SEQUENCE=TGAGGATAGGTTTTGGATGCTC
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=59.610
PRIMER_RIGHT_4_TM=60.451
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_03135 Ps_contig_mira-and-tgicl-ass_franssen_28792
SEQUENCE=TCGGGGGTTGATTGATTAGGTTTAGAATAAGAGAGGGACCACCGGACCCATAAAGTCTCCTAATCCTAACTCTTTCTTCTTCTTCAACTCCTCGAACGTTTCACACACACAAGAAGAAAAGAGAAATCATCGACCATGTCCGACGAAGAGCACCATTTCGAAGCTGCCGCCGACGCCGGAGCATCCAAAACCTATCCTCAGCAGGCCGGTACCATTCGCAAGAACGGTTACATCGTCATCAAAGGCAGGCCATGCAAGGTTGTTGAAGTTTCTACTTCAAAAACAGGAAAGCATGGACATGCAAAGTGTCACTTTGTTGCAATTGATATTTTCAATGGGAAAAAGCTTGAAGATATTGTTCCTTCATCTCACAATTGTGATGTTCCCCATGTGAACCGTACCGACTATCAGCTGATTGATATATCTGAAGATGGTTTTGTGAGTCTGTTGACTGATAACGGAAGTACCAAGGATGATTTGAAACTTCCCACTGATGATGCACTCCTTACTCAGATTAAAGATGGATTTGCTGAAGGAAAAGATCTTGTGGTTTCTGTCATGTCTGCAATGGGAGAAGAGCAGATATGTTCTTTGAAGGACATTGGTCCTAAGAATTAGAGTCATATTCTCTGAACTGGCTATGGCTATGGCTATGGGTGATAGCAAGTATTTTATTCTTATGTTGCTAACAGGTAGTAATTTTTCATACGCATGTGTGCGCAATAAAATTGAAAAGGATGGAAGCTGCCTTGAGCTTGAGGCGAGAAATAGGAACTTTATTTATCTTTTGTCTCTTAAGTTATTAAACCCTGCAGTTTTGGCTGGTTGAATTTGTCATAAATGTACCTTATAACTTTATGCTTTTGTTGAGTTTAATATTGAAACCTTATCACTCTTCTCTAGAAAACC
TARGET=102,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5231
PRIMER_LEFT_PENALTY=0.390220
PRIMER_RIGHT_PENALTY=0.132857
PRIMER_LEFT_SEQUENCE=TAGAATAAGAGAGGGACCACCG
PRIMER_RIGHT_SEQUENCE=TTTGATGACGATGTAACCGTTC
PRIMER_LEFT=22,22
PRIMER_RIGHT=242,22
PRIMER_LEFT_TM=59.610
PRIMER_RIGHT_TM=59.867
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.7530
PRIMER_LEFT_1_PENALTY=0.390220
PRIMER_RIGHT_1_PENALTY=0.362762
PRIMER_LEFT_1_SEQUENCE=TAGAATAAGAGAGGGACCACCG
PRIMER_RIGHT_1_SEQUENCE=CTGCCTTTGATGACGATGTAAC
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=247,22
PRIMER_LEFT_1_TM=59.610
PRIMER_RIGHT_1_TM=59.637
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.7753
PRIMER_LEFT_2_PENALTY=0.390220
PRIMER_RIGHT_2_PENALTY=0.385098
PRIMER_LEFT_2_SEQUENCE=TAGAATAAGAGAGGGACCACCG
PRIMER_RIGHT_2_SEQUENCE=CTGAGGATAGGTTTTGGATGCT
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=200,22
PRIMER_LEFT_2_TM=59.610
PRIMER_RIGHT_2_TM=59.615
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.7753
PRIMER_LEFT_3_PENALTY=0.390220
PRIMER_RIGHT_3_PENALTY=0.385098
PRIMER_LEFT_3_SEQUENCE=TAGAATAAGAGAGGGACCACCG
PRIMER_RIGHT_3_SEQUENCE=CTGCTGAGGATAGGTTTTGGAT
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=203,22
PRIMER_LEFT_3_TM=59.610
PRIMER_RIGHT_3_TM=59.615
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=0.8414
PRIMER_LEFT_4_PENALTY=0.390220
PRIMER_RIGHT_4_PENALTY=0.451213
PRIMER_LEFT_4_SEQUENCE=TAGAATAAGAGAGGGACCACCG
PRIMER_RIGHT_4_SEQUENCE=TGAGGATAGGTTTTGGATGCTC
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=59.610
PRIMER_RIGHT_4_TM=60.451
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_03136 Ps_contig_mira-and-tgicl-ass_franssen_28793
SEQUENCE=CGACGCGGGGGAGTTGTGTTTCGGATTTGTACAGAAGAAAGAAAGAGTGAGTGGATCCATCAAAATGAGCCATAGCATCTTTCACCAAACTTTGCTTTGTCAAACGCAATCGGTTGCTGAGCATCAAAGTAAGCTTGGTTCTCGTGGAATTGTTTGCAGTACTTTATTCCAATCGCAGAGTGTTGATAAAGAAAAGAAGCTTTTGCTCTCTACAAATTTCCGCGGGAGCAGATTGTGTGTTAGGAAACGCAGAGTAACCATGGGGAAGAACCAGTCTATTTCACGCGCTATGTTAACATCAAATGCGGCTTCTGAGCTTTCAGAGAAGTTCAACCTGGATGGAAATATTGAAATGCAGGTCAGTCTTAGTTCTTTAGGATCAGGAGCTGCAACACAAGTAGATGTTCAGGTTTCAAATAGTAGGGGATCTATGGTCTTACACTGGGGAGTTATATGTGAAAGTAAAGGGAAGTGGGTACTTCCTTCTCGTCGCCCAAATGGAACTCAAGTTTATAAGAATAGAGCTCTCAGAACTGCTTTTGTAAAATCTGGCTCAGGATCCTCAGTTAGGATTGAAATTGATGATCCTGCTGCACAAGCCATCGAGTTCCTTGTAATTGACGAATCTCAGAATAAGTGGTATAAAAACCATGGTGATAACTTTCACATCAAGTTACCAGTAAAAGATAAGCCAGTTCCGCAAGTTTCCGTTCCTGAAGACCTTGTACAAATTCAAGCATATATTAGATGGGAACGAAAGGGTAAGCAGTCATACAACCCAGAACAAGAGAAGGAGGAATATGAAGCGGCCCGGCGCGAATTATTGGAAGAAGTAGCTAGGGGTACCTCTGTACAAGATATTCGTGCAAGGTTGACTAACAAAACTAGTGATGTTGAAGTAAAGGAGCCTAACAAACCTAATGCTGCTGAAGTAAAGGAGCCTTCTGTTTCGAAAACAAAGACCATCCCTGATGAACTTGTACAGATTCAAGCATTTATACGCTGGGAAAAAGCTGGGAAGCCTAATTATTCTCCCGAACAACAACTTATTGAATTTGAGGAAGCAAGAAAAGAATTGCTATCTGAGCTTGAGAAGGGTGCTTCGGTGGATGAGATACGTAGGAAGATTACCAATGGAGAGATACAAACTAAAGTTTCCAAGCAATTGAAAACCAAACAATACTTCCAAGCTGAAAGAATACAAAARGGAAAAAAAGAGATTTGACGCAGCTTATTAACAGTAATGCTGCTAAAAACACAGACCAGCAACTTGTAGATGTAGACCAACAATTTGTAGATGCTCCAAAGACTTTGTCAGTAATTGAAAGATATRCCAAAGAAAGGGAAGAATATGATACTGGYRGTTGTTTAAG
TARGET=35,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03137 Ps_contig_mira-and-tgicl-ass_franssen_28796
SEQUENCE=GAGCTCTTTGTGACATATGTCAAAGAGGGAGTTCAACGTAGCTGTTCTATAACTCTTGATGATGTAATCTGATTAAGATAAAGATACCTTAAGCCCCCATAAGTCAGATAATTCAAAATATCTAGTTAAAGTGTCAATCAATCATTGAATCAAGCAGATCATCTTGAAGTTTGAAAGCTCACATAGTCATGTGCTAAATATTTTAGAGTGACCATCTTAAATTGTAAAAGGTAAAGAGTATGTCTTGAAGTACTAAGACAACCACACGCACTTAAAATATTTTCAAACCTTCTCTTCCTTTATTAAAACTTATTAAAAACCTATCCAGACATTTATTTATGTAATTGATTAAAAAGTATTTCTAATCAATATTTTAATTAAGTTAATTTAATAAAAATGTAATATCTTATATCAATATTATTGTATTTTTTTATCTATAAAAAAACCTTAAACACAGAATGAGTATTGTATATGAAGACTTGAGTAATTAGTTACCACAGTGCACAATACTTCATTTTTAAATACTATAAGGTGAAGATGGTGCCAACAAGGATTCACCAACAATATGTGGTGATAAAAGAGATAGATAACCTGAGGCATAAAACAATGCCACCTGGCAGATAGGGTTGCATGCAGTTCATATACGCRGNYNGTTCNAANTCAYRYTTYNGGCGACAAGCTCCTTATTTCATTCATTCATTCATTCTCTATCTTTTTATCATCAACACAAACACAAAACAGTGTTTGTTCCTTTGAAACCATAATAGTAATGGCAACCACACCAGCTTTGTATGGAACTGCTGTCAGCACTTCCTTCCTCAGGACTCAGCCAATGCCAATGTCAGTCACCACCACAAAAGCATTTTCCAATGGCTTTCTCGGTTTGAAAACCAGTCTCAAACGTGGAGATCTTGCAGTTGCTATGGCTTCTTACAAAGTGAAACTAGTTACACCAGATGGAACTCAAGAGTTTGAATGTCCAAGTGATGTTTACATTCTTGACCATGCTGAAGAAGTTGGAATTGATCTTCCTTACTCATGCAGAGCTGGCTCTTGTTCTTCATGTGCTGGTAAAGTTGTTGGCGGTGAAGTTGATCAATCTGATGGTTCTTTCCTTGATGATGAACAGATTGAAGCTGGTTTTGTTCTTACTTGTGTTGCTTATCCTACCTCTGATGTTGTTATTGAGACTCACAAGGAAGAAGATCTCACTGCTTAATTTTTCATTTCTGTTACTGCTATGCTATGATATGATATCATGTTATGTTTGTTTCTACAATGAGTAGTAACTCTATTAATTCCTTCACCTGTTTGTAGTAACATGTGAAGTTAATAATGTTATGTTATGTTGTTTTTTTGTCTCACAATTCTTAAATTTCCTATCTTTTCTTCGGTTGACTATTATATAAAATTTGATATTATTCATCAACGTGTTTTTGTTGTATTAAGCATGGTATTTTCTATAAGTAACTAAAACAAAG
TARGET=688,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03138 Ps_contig_mira-and-tgicl-ass_franssen_28796
SEQUENCE=GAGCTCTTTGTGACATATGTCAAAGAGGGAGTTCAACGTAGCTGTTCTATAACTCTTGATGATGTAATCTGATTAAGATAAAGATACCTTAAGCCCCCATAAGTCAGATAATTCAAAATATCTAGTTAAAGTGTCAATCAATCATTGAATCAAGCAGATCATCTTGAAGTTTGAAAGCTCACATAGTCATGTGCTAAATATTTTAGAGTGACCATCTTAAATTGTAAAAGGTAAAGAGTATGTCTTGAAGTACTAAGACAACCACACGCACTTAAAATATTTTCAAACCTTCTCTTCCTTTATTAAAACTTATTAAAAACCTATCCAGACATTTATTTATGTAATTGATTAAAAAGTATTTCTAATCAATATTTTAATTAAGTTAATTTAATAAAAATGTAATATCTTATATCAATATTATTGTATTTTTTTATCTATAAAAAAACCTTAAACACAGAATGAGTATTGTATATGAAGACTTGAGTAATTAGTTACCACAGTGCACAATACTTCATTTTTAAATACTATAAGGTGAAGATGGTGCCAACAAGGATTCACCAACAATATGTGGTGATAAAAGAGATAGATAACCTGAGGCATAAAACAATGCCACCTGGCAGATAGGGTTGCATGCAGTTCATATACGCRGNYNGTTCNAANTCAYRYTTYNGGCGACAAGCTCCTTATTTCATTCATTCATTCATTCTCTATCTTTTTATCATCAACACAAACACAAAACAGTGTTTGTTCCTTTGAAACCATAATAGTAATGGCAACCACACCAGCTTTGTATGGAACTGCTGTCAGCACTTCCTTCCTCAGGACTCAGCCAATGCCAATGTCAGTCACCACCACAAAAGCATTTTCCAATGGCTTTCTCGGTTTGAAAACCAGTCTCAAACGTGGAGATCTTGCAGTTGCTATGGCTTCTTACAAAGTGAAACTAGTTACACCAGATGGAACTCAAGAGTTTGAATGTCCAAGTGATGTTTACATTCTTGACCATGCTGAAGAAGTTGGAATTGATCTTCCTTACTCATGCAGAGCTGGCTCTTGTTCTTCATGTGCTGGTAAAGTTGTTGGCGGTGAAGTTGATCAATCTGATGGTTCTTTCCTTGATGATGAACAGATTGAAGCTGGTTTTGTTCTTACTTGTGTTGCTTATCCTACCTCTGATGTTGTTATTGAGACTCACAAGGAAGAAGATCTCACTGCTTAATTTTTCATTTCTGTTACTGCTATGCTATGATATGATATCATGTTATGTTTGTTTCTACAATGAGTAGTAACTCTATTAATTCCTTCACCTGTTTGTAGTAACATGTGAAGTTAATAATGTTATGTTATGTTGTTTTTTTGTCTCACAATTCTTAAATTTCCTATCTTTTCTTCGGTTGACTATTATATAAAATTTGATATTATTCATCAACGTGTTTTTGTTGTATTAAGCATGGTATTTTCTATAAGTAACTAAAACAAAG
TARGET=1336,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03139 Ps_contig_mira-and-tgicl-ass_franssen_28798
SEQUENCE=GTCGGATTCATCAAACCCATATCTCTTTTTCGGACTCATCCTTTACTTTTCATATTTATCCCTTTTCTCTCTGTTTCTGATCCATTGCTTCCGATTCTTCCACTTCCAACAACAACAACAACAACAGTAATCGCTGTGCTTTCACGCTTATCCTTTTTCGATTCCACCGAGGAAGAAACTAACACTCAACATGTTCCAGAATTTCACTCTTGTTGTTATGGGCATTTTGGGAAACGTTTTCGCTTTCATAGCTCCGTTGTGGATTGCGGTTATCTTCGGTGTTGTTGTTGGTTGGGCTTGGAAACCTAAATGGGCTATTGAGCCTAATACTTACGATTGGACTAGTTTCTTCAAGTTTCGGGTTCCTTGGAGTAATGATTCCGAGCTTCGTAATCAACCCGGTTTTTCCGTTAGTTCTGGTTCTGCTTCGGAGGGTGAAAAGGGTTTGAGGGGTTTAGTGACGGAAGGTGATTTGCAGAATTTGTGTAAGTTGGTTGAGGAAAAAGATGGCGGACCTGCTTGGATTCAGATGATGGATCGATCTACTCCAACCATGAGTTATCAAGCTTGGCGAAGGGACCCAGAGAGTGGACCGCCACAGTATCGCAGTAGAACAACGTTTGAAGATGCGACTCCTGAAATGGTTAGGGACTTTTTTTGGGATGATGAGTATCGGTTGAGGTGGGATGATATGCTTATACATGCTTCAACTATTCAAGAGTGCGAGATCACTGGAACAATGATTGTGCAGTGGGTGCGCAAGTTTCCCTTCTTTTGCAGTGATAGAGAATATATCATAGGTCGTCGAATTTGGAATGCAGAGCGAGCTTACTACTGTGTCACTAAGGGAGTACCTTGTTCCTCTATGCCGAGGCAGAGCAAACCTAAACGAGTTGATCTTTATTATTCAAGCTATTGTATTCGTGCAGTTAAATCAAGAAAAGATGACCAACGGACTTCATGTGAAATTTTATTGTTTCACCACGAAGATATGGGTATACCCTGGGAAATCGCTAAACTTGGAGTTCGTCAGGGTATGTGGGGAGCTGTCCAGAAGTTTGATCCCGGACTACGTGCGTATGAGAAAGAAAGAGATTCCGGTGCTCCATTGTCGCGAAGTGCTAAAAACGCCAAAGTCCACACCAAAGTAACTGCTGACTATGTGAGATCTTTGGAAGACTCAACCAGTAACTTATTGGAGATTGAAAATCGCGATTCTTCCGACAAACCAACAGGGAGAAACATACCTAAGCTTCTAGTTGTTGGGGGAGCCATTGTCCTTGCCTGCACTCTTGATCAAGGGCTAGTGACTAAGGCAGTTATATTTGGAATAGCAAGAAGGTTCGGAAAATTTGGAAGGAGGTTGTGACATGCGAGACACGTGTTTTTTTGTCGAGAAAATACTGGCCATGTAATTTGTGGCAATAGAGTTAGAAATTGAAATGTGACTTTCTTGCCTTAAATATCTGTTTACTCTGTATCTAGTTGGCAACCATGGTTCTAAGAGAAATGATGTTATTCTTCTCTATATTTGTTTGAAGATACGATGCAGCTTTTAAGAGGAAATATGGACATTGTATATTAGCCACTGAAGGAAAGACCATTGCGCTGCATGAATAATATTCAGGTTTTTCTTGAATTCAAAAANNNCTTCTATTTTTTTACTTTGTAAAATTATTCTTTATGCAGTATTATGGTGTATTTTCAAAAGGNGTTCAGTTAGGGGTTAAAACTTAAGTAAAAAAGGGGTAAATTATTAAGGAAAGTAAATTAATTTTAACCAAAAAAACAAGGTTAAATAAAAGGTAA
TARGET=107,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0737
PRIMER_LEFT_PENALTY=0.040562
PRIMER_RIGHT_PENALTY=0.033137
PRIMER_LEFT_SEQUENCE=CGGATTCATCAAACCCATATCT
PRIMER_RIGHT_SEQUENCE=GTGTTAGTTTCTTCCTCGGTGG
PRIMER_LEFT=2,22
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=60.041
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.1359
PRIMER_LEFT_1_PENALTY=0.040562
PRIMER_RIGHT_1_PENALTY=0.095324
PRIMER_LEFT_1_SEQUENCE=CGGATTCATCAAACCCATATCT
PRIMER_RIGHT_1_SEQUENCE=GAATCGAAAAAGGATAAGCGTG
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=163,22
PRIMER_LEFT_1_TM=60.041
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.1954
PRIMER_LEFT_2_PENALTY=0.162220
PRIMER_RIGHT_2_PENALTY=0.033137
PRIMER_LEFT_2_SEQUENCE=CAAACCCATATCTCTTTTTCGG
PRIMER_RIGHT_2_SEQUENCE=GTGTTAGTTTCTTCCTCGGTGG
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=184,22
PRIMER_LEFT_2_TM=59.838
PRIMER_RIGHT_2_TM=60.033
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.2419
PRIMER_LEFT_3_PENALTY=0.040562
PRIMER_RIGHT_3_PENALTY=0.201304
PRIMER_LEFT_3_SEQUENCE=CGGATTCATCAAACCCATATCT
PRIMER_RIGHT_3_SEQUENCE=AAAAGGATAAGCGTGAAAGCAC
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=156,22
PRIMER_LEFT_3_TM=60.041
PRIMER_RIGHT_3_TM=59.799
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=0.2849
PRIMER_LEFT_4_PENALTY=0.040562
PRIMER_RIGHT_4_PENALTY=0.244324
PRIMER_LEFT_4_SEQUENCE=CGGATTCATCAAACCCATATCT
PRIMER_RIGHT_4_SEQUENCE=CGAAAAAGGATAAGCGTGAAAG
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=159,22
PRIMER_LEFT_4_TM=60.041
PRIMER_RIGHT_4_TM=60.244
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_03140 Ps_contig_mira-and-tgicl-ass_franssen_28812
SEQUENCE=TTCGGGGTACACCAACACATTTCCCATCATCATCTTCTTCTTCTCCGATAGGAAACTTTCAGTTCTCACCCCCCAAAAATGACCAACCTTAAAGTTTTCTTCGATATGACCGTCGGAGGCCAACCCTCTGGCCGTATTGTCATGGAGCTTTACGCTGATACCACTCCTCTCACCGCTGATAACTTCCGTGCACTCTGCACCGGCGAAGAAAGGNGTCGGCACTAGGCGGGAANCCTTNTNATTACAA
TARGET=33,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8268
PRIMER_LEFT_PENALTY=0.762694
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=GGTACACCAACACATTTCCCAT
PRIMER_RIGHT_SEQUENCE=CAGAGTGCACGGAAGTTATCAG
PRIMER_LEFT=5,22
PRIMER_RIGHT=196,22
PRIMER_LEFT_TM=60.763
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=0.8557
PRIMER_LEFT_1_PENALTY=0.762694
PRIMER_RIGHT_1_PENALTY=0.093026
PRIMER_LEFT_1_SEQUENCE=GGTACACCAACACATTTCCCAT
PRIMER_RIGHT_1_SEQUENCE=AAGTTATCAGCGGTGAGAGGAG
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=184,22
PRIMER_LEFT_1_TM=60.763
PRIMER_RIGHT_1_TM=59.907
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.8648
PRIMER_LEFT_2_PENALTY=0.762694
PRIMER_RIGHT_2_PENALTY=0.102129
PRIMER_LEFT_2_SEQUENCE=GGTACACCAACACATTTCCCAT
PRIMER_RIGHT_2_SEQUENCE=TGAGAGGAGTGGTATCAGCGTA
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=171,22
PRIMER_LEFT_2_TM=60.763
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.9525
PRIMER_LEFT_3_PENALTY=0.762694
PRIMER_RIGHT_3_PENALTY=0.189810
PRIMER_LEFT_3_SEQUENCE=GGTACACCAACACATTTCCCAT
PRIMER_RIGHT_3_SEQUENCE=AGTGGTATCAGCGTAAAGCTCC
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=164,22
PRIMER_LEFT_3_TM=60.763
PRIMER_RIGHT_3_TM=59.810
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=0.9525
PRIMER_LEFT_4_PENALTY=0.762694
PRIMER_RIGHT_4_PENALTY=0.189810
PRIMER_LEFT_4_SEQUENCE=GGTACACCAACACATTTCCCAT
PRIMER_RIGHT_4_SEQUENCE=AGGAGTGGTATCAGCGTAAAGC
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=167,22
PRIMER_LEFT_4_TM=60.763
PRIMER_RIGHT_4_TM=59.810
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_03141 Ps_contig_mira-and-tgicl-ass_franssen_28813
SEQUENCE=GGACACCAACACATTTCCCATCATCATCTTCTTCTTCTCCGATAGGAAACTTTCAGTTCTCACCCCCCCAAAAAATGACCAACCCTAAAGTTTTCTTCGATATGACCGTCGGAGGCCAACCCTCTGGCCGTATTGTCATGGAGCTTTACGCTGATACCACTCCTCTCACCGCTGATAACTTCCGTGCACTCTGCACCGGCGAGAAAGGAGTCGGCACTAGCGGGAAGCCTCTTCATTACAAAGGCTCTTCCTTCCACCGTGTGATCCCGAATTTCATGTGCCAGGGTGGTGACTTCACGGCTGGAAACGGAACCGGAGGAGAGTCCATTTATGGAGCCAAGTTCGCCGATGAGAATTTCATCAAGACGCACACCGGTCCTGGAATTCTTTCCATGGCGAATGCGGGTCCCGGGACGAATGGATCTCAGTTTTTTATCTGCACTTCTAAGACGGAGTGGCTTGATGGGAAACATGTTGTTTTTGGTCAGGTGGTTGAAGGAATGGAGGTGGTGAAGGAGATTGAGAAAGTGGGGTCCAGCTCCGGGAAGACCTCGAAGCCGGTGGTTATTGCAGATTGCGGCCAACTCTGAGCTTATAAAACATAACATGCATGATGATGTTTCAGCTATAGTTATTATCTGTTTTTGTTTTCGTTTGTTTAATTTCTGTACTGTTTTGCTTTTGTGTTAATGTTTGTTCTACTACTATCTTTGGTTGAAACTCGATGCTTTAAATTTAAAAATACAAATAATAGATAATTCTTGCTACTTTCATAGTTTCAAG
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1524
PRIMER_LEFT_PENALTY=1.088271
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=GACACCAACACATTTCCCATC
PRIMER_RIGHT_SEQUENCE=CAGAGTGCACGGAAGTTATCAG
PRIMER_LEFT=1,21
PRIMER_RIGHT=192,22
PRIMER_LEFT_TM=60.088
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=1.1813
PRIMER_LEFT_1_PENALTY=1.088271
PRIMER_RIGHT_1_PENALTY=0.093026
PRIMER_LEFT_1_SEQUENCE=GACACCAACACATTTCCCATC
PRIMER_RIGHT_1_SEQUENCE=AAGTTATCAGCGGTGAGAGGAG
PRIMER_LEFT_1=1,21
PRIMER_RIGHT_1=180,22
PRIMER_LEFT_1_TM=60.088
PRIMER_RIGHT_1_TM=59.907
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=1.1904
PRIMER_LEFT_2_PENALTY=1.088271
PRIMER_RIGHT_2_PENALTY=0.102129
PRIMER_LEFT_2_SEQUENCE=GACACCAACACATTTCCCATC
PRIMER_RIGHT_2_SEQUENCE=TGAGAGGAGTGGTATCAGCGTA
PRIMER_LEFT_2=1,21
PRIMER_RIGHT_2=167,22
PRIMER_LEFT_2_TM=60.088
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=1.2781
PRIMER_LEFT_3_PENALTY=1.088271
PRIMER_RIGHT_3_PENALTY=0.189810
PRIMER_LEFT_3_SEQUENCE=GACACCAACACATTTCCCATC
PRIMER_RIGHT_3_SEQUENCE=AGTGGTATCAGCGTAAAGCTCC
PRIMER_LEFT_3=1,21
PRIMER_RIGHT_3=160,22
PRIMER_LEFT_3_TM=60.088
PRIMER_RIGHT_3_TM=59.810
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=1.2781
PRIMER_LEFT_4_PENALTY=1.088271
PRIMER_RIGHT_4_PENALTY=0.189810
PRIMER_LEFT_4_SEQUENCE=GACACCAACACATTTCCCATC
PRIMER_RIGHT_4_SEQUENCE=AGGAGTGGTATCAGCGTAAAGC
PRIMER_LEFT_4=1,21
PRIMER_RIGHT_4=163,22
PRIMER_LEFT_4_TM=60.088
PRIMER_RIGHT_4_TM=59.810
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_03142 Ps_contig_mira-and-tgicl-ass_franssen_28814
SEQUENCE=GTTCCCGTTGCTCCGTTTAACTTTCAGACTCTCTCGCAGGATCAAATAACTAAAACGGTAAAAATTTACTTTGCTACCGTTAGAGTTGACGGAAGAGTTCGTTGGAAGGTTGGTACGTTTATTTCTGGTCATTATCATCTTCTTCTTCTCCGATAGGAAACTTTCAGTTCTCACCCCCCCAAAAATGACCAACCCTAAAGTTTTCTTCGATATGACCGTCGGAGGCCAACCCTCTGGCCGTATTGTCAATGGAGCTTTACGCTGATACCACTCCTCTCACCGNTGATAACCTTCG
TARGET=138,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1353
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.102129
PRIMER_LEFT_SEQUENCE=AGAGTTCGTTGGAAGGTTGGTA
PRIMER_RIGHT_SEQUENCE=TGAGAGGAGTGGTATCAGCGTA
PRIMER_LEFT=93,22
PRIMER_RIGHT=278,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=186
PRIMER_PAIR_PENALTY_1=0.2229
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.189810
PRIMER_LEFT_1_SEQUENCE=AGAGTTCGTTGGAAGGTTGGTA
PRIMER_RIGHT_1_SEQUENCE=AGTGGTATCAGCGTAAAGCTCC
PRIMER_LEFT_1=93,22
PRIMER_RIGHT_1=271,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=59.810
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=179
PRIMER_PAIR_PENALTY_2=0.2229
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.189810
PRIMER_LEFT_2_SEQUENCE=AGAGTTCGTTGGAAGGTTGGTA
PRIMER_RIGHT_2_SEQUENCE=AGGAGTGGTATCAGCGTAAAGC
PRIMER_LEFT_2=93,22
PRIMER_RIGHT_2=274,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.810
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=182
PRIMER_PAIR_PENALTY_3=0.2365
PRIMER_LEFT_3_PENALTY=0.033137
PRIMER_RIGHT_3_PENALTY=0.203324
PRIMER_LEFT_3_SEQUENCE=AGAGTTCGTTGGAAGGTTGGTA
PRIMER_RIGHT_3_SEQUENCE=GTTATCANCGGTGAGAGGAGTG
PRIMER_LEFT_3=93,22
PRIMER_RIGHT_3=289,22
PRIMER_LEFT_3_TM=60.033
PRIMER_RIGHT_3_TM=59.797
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.50
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.75
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=0.2553
PRIMER_LEFT_4_PENALTY=0.153161
PRIMER_RIGHT_4_PENALTY=0.102129
PRIMER_LEFT_4_SEQUENCE=CTTTCAGACTCTCTCGCAGGAT
PRIMER_RIGHT_4_SEQUENCE=TGAGAGGAGTGGTATCAGCGTA
PRIMER_LEFT_4=20,22
PRIMER_RIGHT_4=278,22
PRIMER_LEFT_4_TM=60.153
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=8.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=259
=
PRIMER_SEQUENCE_ID=SSR_03143 Ps_contig_mira-and-tgicl-ass_franssen_28815
SEQUENCE=GACAAAAATGAATNNANTNAGTTAGACATTAACCAATAAATATTTATTTTCTCCACTCCACTCCAATCAAACAATACTATTATTATGATTATTAAGAACATTTCATACTATTATATTTTCTCCACGTTACAAGGAATAACATCACATCACTCACTATAAGTAAACAAAACTATATATACTAATTTGAGAAACTAATACACTTCACTTTAACCTTTTTTTACTTTGCTTGCTTCCCCTTACACAAACATGTTTAATATATTTATTTATAGTAACGCATAATTAAACTGTTGCAATTGGTCCCAAATTAACAACATTTGATTCCTTAAATGCTTCAAATCTTGGTTCTTTGGCCTGAAACTTTAACCCAGCGTAGAGTTTCATGTAATCCTCAAACACAAATTTTGGGTAAATTTCATTGTTTTCTTCTATCAATGTTGGTGCTGGGTAGATAACAGCGTCACTACCAGGGTTGTAGAATGAGGCTATGGACATTCTTGTTCCATCACTTTGTGCTATCACACGGTGCTCTATACTTTTGTATTTACCATTGGTAATTACCTCTAGTTGGTCACCGAGGTTGATGACAATTGAATGATGCATGGGTGGAACATCTACCCAGATGCCATCTTTGAGAAGTTGGAGGCCACTGACTTTGTCGTCTTGGAAAAGGAGGATGATTCCTCCGGCATCGGTGTGTGCGCGGAGACCCTTCACTAGATCGGGTTTTGGACATGGAGGGTAGTTTGCTATCTTGGTGCCAAAAGTTGGTCCTTTTGATCCATAGAAGGCCTTTTTGAGGTACCCTTTTTCTAGTCCAAGATTTTCACATAACAAGTCTAGTAGCTCCTCTGCTAGTTTCTCTAGTCTCAAAGCAAATTCCTTCATTGATTTCCTGTATTCATCACTAAGATCAGGGATCTCTGAAATGTTTGACTCAGGTAGGTGACGCAAGTGGAAGGTACTCTCCCAATCCATGTCTTTGACCTCAGTTTGAACAGCTTCAAGCCCTTTCTCAGCTACTAATTCCTTGAATCTTTGTTCCATGCATTTCCTGTAGTGTTCTTTGGTCAATCTCTCCACGGTGTCCATCAAGTCATGAGGTATGCCATGATTCACCAGCTCAAAGAATCCCCAGTTTTCACAAGCATCTTTGATTTTCTCCATAGTAGCTTCTCTCTTCTCACCATTGATGTTCTCTAAGTTGATAATTGGGAAATTCTCCATATCTCACTCAAACTCTTAGCTTCAACTCTCTTTGTATGCTTTTCTTTCTCCAAGTTTATTGTAGGTTGCTTGCTTTAGGATCCACTCCCGA
TARGET=51,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03144 Ps_contig_mira-and-tgicl-ass_franssen_28818
SEQUENCE=TGTGCGGAGATGAGGATATTATCTTTCTCTGGACTTAGTGAAACTCATATTGATGGTCTTGTGGGACAGGCATTGTTTGGAGATGGTGCGGCAGCTGTGATTATTGGTTCAGACCCATTGCCAAAAGTTGAAAATCCTTTGTTTGAATTGGTATGGACTGCACAAACCATCCTTCCAGATAGCGGAAGATATATATATTAAAGGTGACCTTCGAGAAGTAGGAATGATATTATATCTTGAACAGGA
TARGET=189,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3298
PRIMER_LEFT_PENALTY=0.081801
PRIMER_RIGHT_PENALTY=1.248025
PRIMER_LEFT_SEQUENCE=ATTATTGGTTCAGACCCATTGC
PRIMER_RIGHT_SEQUENCE=TCATTCCTACTTCTCGAAGGTCA
PRIMER_LEFT=99,22
PRIMER_RIGHT=226,23
PRIMER_LEFT_TM=60.082
PRIMER_RIGHT_TM=60.248
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=1.3953
PRIMER_LEFT_1_PENALTY=0.147295
PRIMER_RIGHT_1_PENALTY=1.248025
PRIMER_LEFT_1_SEQUENCE=TATTGATGGTCTTGTGGGACAG
PRIMER_RIGHT_1_SEQUENCE=TCATTCCTACTTCTCGAAGGTCA
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=226,23
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=60.248
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=1.4397
PRIMER_LEFT_2_PENALTY=0.191680
PRIMER_RIGHT_2_PENALTY=1.248025
PRIMER_LEFT_2_SEQUENCE=TCATATTGATGGTCTTGTGGGA
PRIMER_RIGHT_2_SEQUENCE=TCATTCCTACTTCTCGAAGGTCA
PRIMER_LEFT_2=44,22
PRIMER_RIGHT_2=226,23
PRIMER_LEFT_2_TM=60.192
PRIMER_RIGHT_2_TM=60.248
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=1.4734
PRIMER_LEFT_3_PENALTY=0.225403
PRIMER_RIGHT_3_PENALTY=1.248025
PRIMER_LEFT_3_SEQUENCE=GCAGCTGTGATTATTGGTTCAG
PRIMER_RIGHT_3_SEQUENCE=TCATTCCTACTTCTCGAAGGTCA
PRIMER_LEFT_3=90,22
PRIMER_RIGHT_3=226,23
PRIMER_LEFT_3_TM=59.775
PRIMER_RIGHT_3_TM=60.248
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=137
PRIMER_PAIR_PENALTY_4=1.6210
PRIMER_LEFT_4_PENALTY=0.081801
PRIMER_RIGHT_4_PENALTY=1.539185
PRIMER_LEFT_4_SEQUENCE=ATTATTGGTTCAGACCCATTGC
PRIMER_RIGHT_4_SEQUENCE=CATTCCTACTTCTCGAAGGTCA
PRIMER_LEFT_4=99,22
PRIMER_RIGHT_4=225,22
PRIMER_LEFT_4_TM=60.082
PRIMER_RIGHT_4_TM=58.461
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=127
=
PRIMER_SEQUENCE_ID=SSR_03145 Ps_contig_mira-and-tgicl-ass_franssen_28827
SEQUENCE=GTTCGGGGTTACTACTACTACCTACTAAATTCGCAGCCACCACCGTTAACTGGAGAAGCGACCATGGTAGGTTTCAACTCTCAGTCGTCCTACGGTATGCAATCCATGCCCAAAGAAGGTCACAAGCATCTCTCCGGTCTCGACGAAGCCGTTCTCAAAAACNTCGATGCCTGTAACCAACTCTCCACCATCACTCGCACTTCCCTCGGCCCCAACGGGATGAATAAGATGGTTAANAAAT
TARGET=10,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03146 Ps_contig_mira-and-tgicl-ass_franssen_28834
SEQUENCE=TTTCCTTTCCTTGGAAGAACTAAGTCTCATATTTATCGGACGTTCCCAACCCCTCGAGCCGGATCTTTCAACATTACAATAAATCTAACTTGATATAAAATTCCTGGTGAAAGCTTTCTAGTGTCAAATTTTCCATGCACTCCTAGCCAACAAACTTTCTTCAGTTCAACTACCTCAGGCAGCCCTTCATCATTTTGCGGTATCCATTTCCAGTAAATTGGATTCTCTGCCCAAGTGATTGATAGTGCTCTTGCAAACAAAACAAAACAATTGGCGTTGGTCTTCTTATCAACCCAATACAACTGAGTTTTATCATGTAAGTACACACCATCATGTAGTTCATCAAACAACTTTTCCTTGGAAGATATATCAACTGGTGAGTCTGCATCCATTAATATGCGCTCAAAGTTGTGAGGAAAACTTAGTTGTGCTGATTGAGTCTCATATTTGGCTTTTACAGCTGGGYCCTTTTGTACCACCTTTCTGGAGGGTTCTATGTTTTGTTGAGGAGACAGCAACAAGTTTTTCCCTGTGGAAGATAATCGTAGTTTTACCTTAGTTTTGATTGGTGGTTCTTTTGGTTGGTGATGCTTTTTTGTTGCTGTGACTGTGAATGATGATGTGTTTGAGGATGTTGGTGCTGCTGCTCTAGCTCTGATAGAGAACGCCCCCAGAGGATGTTGGTGCTGCTGCTCTAGCTCTGATAGAGAACNCCCCATTTCAGTTGGTATGCTATAYCNATTTGGGTTTCACCCTGAAGCTTTACAAAATCTGATCGCTGTTCTCTGTTAAGATTGCACCTGTCAATGAATACACTCTTCCCATCCTTTAATCCTCTGGCTGCACTACTTAAACATTGAGCTTTATTTCCTGCTTTACCATTTCCAAT
TARGET=255,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03147 Ps_contig_mira-and-tgicl-ass_franssen_28838
SEQUENCE=AACTACAGTTGTATTGGGTTGGTNTAAGAAGACAACGCCAATGTTTGTTTTGTTTTGTCAAGAGCACTATCAATCACTTGGGCAGAGAATCCAATTTACTGGAAATGGATACCGCAAAATGATGAAGGCGACGGGATGCCTGAGCTAGCTGAACTGAAGAAAGTTTGTTGGCTAGGAGTGCATGGAAAATTTGACACTAGAAAGCTTTCACCAGGAATTTTATATCAAGTTAGATTTTATGTAGTGTTGAAAGATTCGGCTCAGGGTTGGGAACTTCCGATAAATGTGAGACTAGTTCTTCCAGGAGGAAAGAAGCAGCAACACAAGGTGAATTTAATGGAGATGTTAAGGGAAAGTTGGACGGTGGTTCCGGTAGGTGAGTTTGTAGCAGGTGAGAAAGATGGTGGTGAGATGGAGATATCTTTGTTTGAATATGATGGTGGTACGTGGAAGCAGGGGCTTGTCATCGGAGCCATTATAATCAAACCAAAAATATATAGTTATGTAATGTGGCAATAAAGCTTAGTTTCTTTTGTAATAAATAAAATAGTCCTTTGGATTGTTCATAATATTATGCATACTATACTGCAGTGTGTCATTGTCAGGAGGAGGGGTGATGGCTCTATGTTTGACACACATGGGACTATTTGTCTCTGGCAATGTTTACATARNTYGCCCCAACTAGTATCATATTATTTTTAGTGTTGCTCTATATATTTCTGTTGAGTAGTAAA
TARGET=44,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03148 Ps_contig_mira-and-tgicl-ass_franssen_28842
SEQUENCE=AGTANGNRRGGTAAACAAAGCAAAATTGGACTCCATTACTCTCTTAAAGCTTCTAAAAGATTCCAATGGCATGCTTCCAAAAAAATCTCCACTTATCTTGTGATCTATACGCATTATGCATGCATCCATAAAGCCATCGGATTATTATATTTTAGATCACTACCTAATAATCAATATAAATCTATCAGAATAGGGAATTGATTTTTGTTAAGAACCTAACATGTCGGAAGTTAAGAACAAGCAAGTCATTCTGAAGGATTATGTTTCTGGCTTTCCTAAAGAATCAGACCTGAACATCATCAGCAGTACCATAACTCTAAAGATTCCAGAAGGTTCCAACGATGTTGTTCTGCTAAAAAATCTCTACTTGTCGTGTGATCCTTACATGCGGAATCTCATGAACAACCCAAAAGACTCTCTCTCTTCTCGTGCCTTTACTCCTCAGTCTCCGTTAGCTGGTTATGGTGTGTCTAAAGTTCTGGAATCTGGACACAAAGATTATAAGGAAGGTGATTTGGTCTATGGTGTTACTAATTGGGAAGAGTACAGCTTGATCCCTGCAGCTCAAATAATTTTCAAGATTGAGCATACAGATGTTCCTCTTTCCTACTATACTGGAATTCTTGGTACGCCAGGGCTTACTGCGTATGCAGGTTTCTTTGAAGTGGGAACTCCCAAGAAAGGAGAGAATGTTTTTGTTTCAGCTGCCTCTGGTGCTGTTGGTCAACTTGTTGGCCAATTTGCTAAATTGCACGGTTGCTATGTCGTTGGAAGTGCCGGAACTAAAGAAAAGGTGGATCTGTTGAAGAATAAATTTGGATACGATGAAGCCTTTAACTACAAAGAAGAACCAGACCTCAATGCAGCTTTGAAAAGATACTTTCCCGAAGGCATTGACATTTACTTTGAGAATGTTGGAGGAAAGACACTCGATGCTGTGCTCTTGAATATGAGAATCCATGGTCGCATACCTGTGTGCGGAATGATCTCACAGTACAATCTTACGCAACATGAAGGCATAACCAATTTGGCACATATCATATATAAGCGGATTCGTATCCAGGGTTTTATTGTAGCTGATTTCCATCACCTGTATCCCAAATTCTTGGAGTTTATCCTGCCTCATGTAAGAGAAGGGAAGGCTGTGTATGTTGAAGACATAGCTGAAGGACTTGAGAAAGGACCTGCAGCATTGGTGGGAATATTTAGTGGTCAGAATGTTGGTAAACAAGTGCTTGTTGTTGCTCGTGAGTGAACTCTGATGCTTTCATTTGATTTTTGTGTGTGTTGAATAACAAGCTCTCTTTGTATGATGGCCTTTTGCAATAGTATTTCTCTTCTCTAGTGTTGATTGTAATTGCTTGCTTATGTGATATCTTGTGATTAATAAAGCCTGTTATATGCTTCTCTTAACG
TARGET=415,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03149 Ps_contig_mira-and-tgicl-ass_franssen_28855
SEQUENCE=TAGNGAATTAAACCCTAGTTACCAATAATTTTGGAAATACCGTTATCCAACTTTCACATCAGTTTTTCGCGTTCACGAATTCACTTCAAAATCGAATTTCACAATAATAAGTAGTAACAAACAATTGAGATTAACCTCAGTGTCTGCACTTCTCAACCACAGAAATGGCTTCCATGGCTTTCTCCACAACCTTCTTCACTCCTCTTCGTGATTTCAACCAACCTAGAACCAATTCAACCCCTTCAACTTCTTTGCCTTTTACCAAATCCTCAATTGCTTCTTCTAAGTCCCCGTTTTTTCAACTAGGGTTTTCTCAACAAGCTTCCTCCAATTTCCCAATTGTTCCGTCGAAGACTAGGTCTTTCTCAGTCAACGCTAAGGCTATCAAAGATAAAACCATTTATGATTTTACTGTTAAGGATATTGATAAGAAGGATGTTTCTCTTAGCAAATTCAAGGGGAAGGTTCTCTTGATTGTCAATGTTGCTTCACGATGTGGCCTGACATCATCAAACTACACAGAACTTTCGCACTTATATGAAAATTTTAAAAATAAAGGTTTGGAGGTTCTAGCTTTCCCTTGCAATCAATTTGGCATGCAGGAGCCTGGATCAAATGAAGAAATTAAGCAATTTGCTTGCACTAAGTTCAAAGCAGAATTTCCAATTTTTGATAAGGTTGATGTAAATGGACCATTTACTGCTCCAGTATACCAGTTTCTGAAATCAAGTTCTGGGGGCTTTTTCGGTGATATTGTCAAGTGGAATTTCGAGAAATTCTTGGTTGATAAAAATGGTAAAGTTGTTGAAAGATACCCACCAACAACCTCTCCTTTTCAAATTGAGAAGGATATCCAGAAGTTACTTGCTGCATAAGTATATTCAGGAATGAGGAAAATTGAAGCCATGATTGCACAAGGTCACAAGTAGTTAAAGTTTATCAAAAACAATAAAGATGATTATTATTATTATGTAAATTTGTTTGGCATAAAGAGAAGAAAAATATTACTTTACCGCTAAAGGAATGAAGTTCTATATGGTCAGAATTTTTGTTCAAAYTTTAGACTCTACAATTT
TARGET=958,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03150 Ps_contig_mira-and-tgicl-ass_franssen_28856
SEQUENCE=CATTTTAACAAGTAACAAAACCCTAAATAACRATAGTCATCAGGGGAGTCAGGTTCAAACAACTATATCACATACAATTTGGACTCGCACCGATATCATATCAACGCAGACGGAGTTTTGAGCAAGTCATCTTCCAGGAAGCTTATATAAACTCTTTTAGAAGGAAAACACCGTCTCTGAAAGAAAGAAAAAACCATTACTTCATTCTCTTTAAGTACTGATACTGGGAAATAATCCAAAAACATGAGTCGAGGAAGTGGAGGTGGATACGATCGTCACATCACTATTTTCTCCCCCGAGGGGCGTCTCTTTCAAGTCGAGTATGCTTTCAAGGCTGTTAAGGCTGCAGGGATAACTTCGATTGGTGTCCGAGGAAACAATTCTGTCTGTGTCGTTACTCAGAAAAAAGTTCCGGACAAACTCTTGGACCAGACAAGTGTAACACATCTCTTTCCCATCACGAAGTACCTCGGTTTACTGGCAACTGGCATGACAGCTGATGCTAGGACACTGGTTCAACAAGCAAGAAATGAAGCAGCCGAGTTTCGCCATAAATATGGATATGAGATGCCTGTAGATGTGTTGGCTAAATGGATTGCAGACAAGTCACAAGTCTATACTCAACATGCTTATATGAGACCACTTGGAGTAGTTGCTATGGTCTTGGGTATTGATGACGAAAATGGGCCTCAGCTTTACAAATGTGACCCTGCTGGTCATTACTTTGGTCACAAGGCCACAAGTGCTGGATTGAAGGATCAAGAGGCAATTAATTTCTTGGAGAAGAAGATGAAGAACGACCCTTCATTTACCTATGAGGAGACAGTTCAGACTGCAATTTCTGCCTTGCAATCAGTTCTTCAAGAGGATTTTAAGGCAACTGAGATTGAGGTTGGAGTGGTGCAAAAGGACAAGCCAGAATTTAGAGTTTTGTCAACTGAGGAAATTGATGAGCATTTGACTGCCATCAGTGAGCGTGATTGAGTTAAGAAGGATATGACTCTTAAGACTTTGTGATGTTCAATTAAATCATGTATACTTATTGTCTTGATTCACTAAATATACATCCTTATGATATTTGAGTATCACTGATGTTGAAGACTGTATTTGCTTCTGTTACAAGTTGGGGCCTGCTAATCAATCATGTTTACATCTGCTATCCTTAAAACGTTGTACAACCCATGTGTTTGATTGAAGAAGCTAGAATGTTGAGATGAAAAGAGTAATGATGGTCCTCTCCT
TARGET=179,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03151 Ps_contig_mira-and-tgicl-ass_franssen_28858
SEQUENCE=TCGGGGGCTTACAAAGCCAAGTAGAACGCTTCATCCTAATATCATTCTCTCTCTCATTTCTCATTCACTCTTCGAACCTCGCGATTTCAAAAAGGAACCATGGGTCAAGGAACACCAGGCGGTTTGAACCGGCAAGGTTTACCGGGTGACCGGAAACCAGATGGCAGCGACAAAAAAGACAAGAAATTCGAGCCGGCAGCGCCACCGGCTCGCGTCGGTCGCAAACAGCGAAAACAGAAAGGTTCAGAGTCGGCGTCGCGGTTGCCGACGGTAACTCCTCTGTCGAAATGCAAACTGAGGCTCTTGAAACTCGAACGAATCAAAGACTATTTGTTGATGGAAGAAGAGTTTGTAGCGAATCAGGAACGGCTTAAGCCTCAGGAAGAGAAGGCGGAAGAGGATAGATCTAAGGTCGATGATCTTAGGGGTTCGCCTATGAGTGTTGGTAACCTTGAGGAACTCATTGACGAGAATCACGCTATTGTTTCTTCTTCGGTTGGACCTGAATATTATGTTGGTATATTGTCTTTTGTTGATAAAGATCAGTTGGAACCTGGATGCTCTATTTTGATGCATAACAAGGTTCTTTCTGTTGTTGGGCTTCTTCAAGATGAAGTTGATCCAATGGTTTCTGTCATGAAGGTAGAGAAGGCTCCTTTAGAATCATACGCTGACATCGGTGGTTTAGATGCCCAGATACAGGAAATCAAAGAAGCCGTTGAGCTTCCCCTGACACATCCTGAACTGTATGAAGATATTGGTATCAAGCCTCCAAAGGGGGTCATTTTATATGGAGAACCTGGAACCGGAAAGACATTGCTTGCAAAGGCTGTGGCTAACTCAACATCAGCAACATTCCTACGTGTTGTTGGTAGTGAATTGATACAAAAATACTTAGGAGATGGTCCAAAACTTGTGCGGGAACTTTTCCGAGTTGCTGACGATCTTTCTCCTTCTATTGTCTTCATTGATGAAATCGATGCTGTTGGAACAAAGAGGTATGATGCTCACTCAGGTGGAGAGCGTGAAATTCAAAGGACAATGTTGGAGTTGCTTAACCAGCTAGATGGTTTTGATTCTAGAGGAGATGTAAAAGTTATTCTCGCAACCAACAGAATTGAGAGCCTTGATCCAGCTTTGCTACGACCAGGTCGAATAGACAGGAAGATTGAATTTCCTCTCCCTGATATAAAAACAAGGAGACGCATTTTCCAGATACACACATCAAGGATGACATTAGCCGATGATGTCAATTTAGAAGAATTTGTTATGACTAAGGATGAGTTCTCTGGAGCTGATATAAAAGCAATATGTACCGAAGCTGGCCTACTTGCTTTACGAGAACGCCGCATGAAGGTGACACATCCTGACTTCAAGAAGGCAAAAGATAAAGTCATGTTTAAGAAGAAAGAAGGGGTGCCAGAAGGATTGTATATGTGAAATATCTTCCTCATTTACTCTGCCTCCTTTGTTTTATCATTGTTTTTTTCCTAGTAATCACAGGTGTACTATTCAGCATTGTGAATTGTGGTCGGTTATTGGGCCTGAACTATAGTTTTTTCAATTCAAAAAATGGGAATTCTTCCATGATTCTGAGCAGACTTTCATTCATATTAACAGCTCACATTCCTTTTCTTCATGGTATTGTTCACTGGTCACGACAATTTGGTTGTCTAAGCAGTATAAAGCTACTCATGAGATCGGATTTAGTTCTATGTAGAGATGACAGCTAAACTCCTGTCCATTGTCAATAACTTATAAATATTAGTACAAAAAATTAAAANNNTTT
TARGET=46,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1331
PRIMER_LEFT_PENALTY=0.080887
PRIMER_RIGHT_PENALTY=0.052187
PRIMER_LEFT_SEQUENCE=AAGCCAAGTAGAACGCTTCATC
PRIMER_RIGHT_SEQUENCE=CTCAGTTTGCATTTCGACAGAG
PRIMER_LEFT=13,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=59.919
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.1349
PRIMER_LEFT_1_PENALTY=0.080887
PRIMER_RIGHT_1_PENALTY=0.054021
PRIMER_LEFT_1_SEQUENCE=AAGCCAAGTAGAACGCTTCATC
PRIMER_RIGHT_1_SEQUENCE=CGAGTTTCAAGAGCCTCAGTTT
PRIMER_LEFT_1=13,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.919
PRIMER_RIGHT_1_TM=60.054
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.1417
PRIMER_LEFT_2_PENALTY=0.080887
PRIMER_RIGHT_2_PENALTY=0.060824
PRIMER_LEFT_2_SEQUENCE=AAGCCAAGTAGAACGCTTCATC
PRIMER_RIGHT_2_SEQUENCE=AGTTTCAAGAGCCTCAGTTTGC
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=310,22
PRIMER_LEFT_2_TM=59.919
PRIMER_RIGHT_2_TM=60.061
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=0.1982
PRIMER_LEFT_3_PENALTY=0.080887
PRIMER_RIGHT_3_PENALTY=0.117281
PRIMER_LEFT_3_SEQUENCE=AAGCCAAGTAGAACGCTTCATC
PRIMER_RIGHT_3_SEQUENCE=TTGCATTTCGACAGAGGAGTTA
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=292,22
PRIMER_LEFT_3_TM=59.919
PRIMER_RIGHT_3_TM=59.883
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.2184
PRIMER_LEFT_4_PENALTY=0.080887
PRIMER_RIGHT_4_PENALTY=0.137489
PRIMER_LEFT_4_SEQUENCE=AAGCCAAGTAGAACGCTTCATC
PRIMER_RIGHT_4_SEQUENCE=GCATTTCGACAGAGGAGTTACC
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=290,22
PRIMER_LEFT_4_TM=59.919
PRIMER_RIGHT_4_TM=60.137
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_03152 Ps_contig_mira-and-tgicl-ass_franssen_28867
SEQUENCE=TTCTTCTCTCCTCGTCCGATCCCAACTACCACTCANAGAGATTATGGTGCGCGCAGTAGCAAACGACGTCGCCGCTCTTCCTTCCTCCTTTTCGTAACCAACGGAAACGGGACGGACTGCGGCAACAACAACAGTAACACACGCGTCGCCGCCTCTCCCATCCTCCTCTTTGAACCAAGCAACAGCGACTGCAACAACAACAGCAGCAGCAGCAACAGCGTGTGAAGTGTCGAATGCGCYGAAGAAGAAGAAAATACTGCTGTGCTTGTCCAGACACGAAGAGGCTTCGGGATGAGTGCATCGTGGAGAACGGAGAAGACGCTTGTGCCAAATGGATCGAAGCTCATCGACTCTGTCTCCGATCCGAGGGATTCAATGTTTGAGAATCGAAAGTTGGAAAAGATGGACCATGACCAGACTGGATGCGAAGCAGCCCCTGAAGGTCCTATGCTGTGCGTCAACAACTGTGGATTTTTCGGAAGCGCATCTACCATGAACATGTGCTCTAAGTGCCACAAAGACATGATGCTGAAACAGGAGCAGGCAACACTTGCAGCATCTTCCATTGGGAACATTATG
TARGET=201,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03153 Ps_contig_mira-and-tgicl-ass_franssen_28867
SEQUENCE=TTCTTCTCTCCTCGTCCGATCCCAACTACCACTCANAGAGATTATGGTGCGCGCAGTAGCAAACGACGTCGCCGCTCTTCCTTCCTCCTTTTCGTAACCAACGGAAACGGGACGGACTGCGGCAACAACAACAGTAACACACGCGTCGCCGCCTCTCCCATCCTCCTCTTTGAACCAAGCAACAGCGACTGCAACAACAACAGCAGCAGCAGCAACAGCGTGTGAAGTGTCGAATGCGCYGAAGAAGAAGAAAATACTGCTGTGCTTGTCCAGACACGAAGAGGCTTCGGGATGAGTGCATCGTGGAGAACGGAGAAGACGCTTGTGCCAAATGGATCGAAGCTCATCGACTCTGTCTCCGATCCGAGGGATTCAATGTTTGAGAATCGAAAGTTGGAAAAGATGGACCATGACCAGACTGGATGCGAAGCAGCCCCTGAAGGTCCTATGCTGTGCGTCAACAACTGTGGATTTTTCGGAAGCGCATCTACCATGAACATGTGCTCTAAGTGCCACAAAGACATGATGCTGAAACAGGAGCAGGCAACACTTGCAGCATCTTCCATTGGGAACATTATG
TARGET=241,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03154 Ps_contig_mira-and-tgicl-ass_franssen_28886
SEQUENCE=ACTTGATTTGGAACAAGTGTGTGCAGAGCTTAGTCTTCATTGGGTACCCTTTTGTGTCGAGACGAAGAAGTGGTTTTAAGGTTGAAGCGGGTTGGATGTTTAGAGGAGGAGGAGAACAAGGGTTGGATGCAAGTGTAGAGCAGAGTGAAAGTGCTAATGAGGATATCTTGATGTTCTTCTTTCAGCTGGATTTAGCTACTAGAGTGCAGTGTGCTTTAAACATGGAAGAGTATGACATTGCACAGCAGCTGCGAAACAAACTTACTGAGGTTGAAGAAGAGGTAATTAAGCAACAACAGTCAAAAAGGGGATTGTCTTCAAAAAGTGATGCACAAGATAAAGGTCTAAGTATTATACGTCTTCGTTCAGACCTTCAGAGTGCTATTGAGAATGAGGATTATGCCTTGGCTGCTAAATTACGTGATGAAATTTCCAAACTCGAAGCAGAGTCTCTGGCTGTGTCAGCTAAAGCCTTAGCACATGAAAATGCACAATATGTGTTTCGATTGGGGCAGAAAGTAAAGCATAAAATATTTGGCTATAGAGCTATAATTGTAGGAATGGATCCAGTATGCAGTGAATCAAATTCATGGATGGAAAATGCACAAGTTAAAAAGTTGTCTCGTGGTTCCACTCAACCATTTTATCAGGTACTTGTTGACGTACGTGCTGAACCAGATCTACTGGTGGCGTATGTTGCAGAGGAGAATCTTTTAATACCTGACAAACCAGACAAAGGAAGATTTGATCACCCCTACATGTCTTTTCTGTTCTATGGAATGGATTCAGCTGGAGATTTCATCCCTATAAAGCAACTGCGGGAGAAGTACAACAAACCACGCCACGAAATACCCTTTGATGAAGATGGACAGAAAGCTTAAATTACTAATATGAGGAGGACTATATGCAATGCTCATGCCTGCTTCTGGTGATTTGAGATTGAAAATTTTGTTACGATATATTGAAAGAATTAGTCGAACCGCAATGGAGAAAAGTAGACAACTTATTGACCAGAAAGCATTTCCTTGAGTTGTTCATACAATGATGGCTAGTGGCTACCTCTGTCTAAGGTTATTTCATTCTGTATGATTTATCAGTAATGAAAGGTACTAACTACTTAAAGCCTGCCTTCCGTCACATGTCTGTACATAATGGTAATTATAAAATGACAGAGGTTGTGTTTATAATTCACAAATGTTCCAACATATCTTGCTTAAAGTTTTAATCCTAATTATTATCTAAC
TARGET=103,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2122
PRIMER_LEFT_PENALTY=0.205226
PRIMER_RIGHT_PENALTY=0.006945
PRIMER_LEFT_SEQUENCE=GTCGAGACGAAGAAGTGGTTTT
PRIMER_RIGHT_SEQUENCE=TCCAGCTGAAAGAAGAACATCA
PRIMER_LEFT=55,22
PRIMER_RIGHT=189,22
PRIMER_LEFT_TM=59.795
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=0.2336
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.028405
PRIMER_LEFT_1_SEQUENCE=GTCGAGACGAAGAAGTGGTTTT
PRIMER_RIGHT_1_SEQUENCE=AGCTGCTGTGCAATGTCATACT
PRIMER_LEFT_1=55,22
PRIMER_RIGHT_1=249,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=59.972
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=0.2659
PRIMER_LEFT_2_PENALTY=0.258908
PRIMER_RIGHT_2_PENALTY=0.006945
PRIMER_LEFT_2_SEQUENCE=TCTTCATTGGGTACCCTTTTGT
PRIMER_RIGHT_2_SEQUENCE=TCCAGCTGAAAGAAGAACATCA
PRIMER_LEFT_2=33,22
PRIMER_RIGHT_2=189,22
PRIMER_LEFT_2_TM=59.741
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.2873
PRIMER_LEFT_3_PENALTY=0.258908
PRIMER_RIGHT_3_PENALTY=0.028405
PRIMER_LEFT_3_SEQUENCE=TCTTCATTGGGTACCCTTTTGT
PRIMER_RIGHT_3_SEQUENCE=AGCTGCTGTGCAATGTCATACT
PRIMER_LEFT_3=33,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=59.741
PRIMER_RIGHT_3_TM=59.972
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=0.3041
PRIMER_LEFT_4_PENALTY=0.297164
PRIMER_RIGHT_4_PENALTY=0.006945
PRIMER_LEFT_4_SEQUENCE=AAGTGGTTTTAAGGTTGAAGCG
PRIMER_RIGHT_4_SEQUENCE=TCCAGCTGAAAGAAGAACATCA
PRIMER_LEFT_4=67,22
PRIMER_RIGHT_4=189,22
PRIMER_LEFT_4_TM=59.703
PRIMER_RIGHT_4_TM=59.993
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_03155 Ps_contig_mira-and-tgicl-ass_franssen_28887
SEQUENCE=ACACACAGTTGCACTTTATAGTATGTGCAATTTCCACACAGATCGGTGACACTCATTAATTTATCTCTCTCACCAATCACCTTCTCCATCCTTTCCCTTTAAACAAGTTCGCAACTTTTTTGTTCTTTCTGCAACACTACCTCGGTTCACTCACTGGGTCATCCTCCAATTCTGTTTTAGAGCCACACAACACAAAGATTCCTCTTTGACTTTCAAGCTACAAATAGCTGTTGTTTTTGATCATGGATTCCCCACAATCTGTTGTGTCACCCTTTAGAAGCTCAATCTTGGGTGAGGGTGAGAAGCACAAGTCTGATGTTTTTGCGCAAAGCAACAGTCCTTCCTCTAAGGAGATTGAAGTCAACAGGAAGGAAACTATCATGTCAAATGCCGATGAATGTGTTGGAGTGCTTGATGTTTACATACATCAAGCTAGGGATATTCAGAACATCTGCATATACCATAAGCAAGATGTTTATGCTAAGATTTGCCTGACGAATAATCCTGAGAATGCTGTTTCTACGAAGACTATCAATGGAGGTGGAAGGAACCCTGTGTTTAATGACAATCTTCGTCTCAATGTTTGTAACGTTGATTCGTCTCTTAAATGTGAGTTATGGATGCTTAGCAGGGTGAAGAATTATCTCGAAGATCAGTTGCTTGGTTTTGCTTTGGTGCCGTTGTCTGAAGTACTAGTTCAGAATGGGAAGCTAGAGAAAGAGTTCTCGCTTTCTTCAACTGATCTATTTCACTCTCCTTCAGGGTTTGTTCAGTTGTCAATTGCTTATACTGGTGCTACGCCTGATGTTATGGCAATATCGGCAATGCCTGGCGAGGAGGCCACACGTGCTGCTGCGCAGGACTTGGAAACATCCGAGTCATTGGCCAGAGATTTGGGTAAAATTGAGTTTCCAGATCCTAAGATCGCGAATGAAGATCACTTAATGGTTTCAGAATATTTTGGCATTTCGTGCGAGGAGAGTCAATGCTCTGATAGCTTAGCCAATTCTGATGCTGACAATCATAGTTCTGAAGCCGGTGTTCGTCTAGTGGAAAGCTTCTCAGCATGTAGCGGTGAGTCTGTGCAAGTGCAACCTCATAAGGTTGATTCTCCGCCGAGCAGTGTGTCGACAAATGGGGTTTCTTCTTCTGCACCCGAAAGCTCCGAATCATCTGATGCTGCTGCTGCAGCTTCAAAGTCCCCGGGTGATGAACAAGTTTCAGGAACCAAAGAGGTACAAAAAGTGGATGTCAAAGATGGTGAGAGTGATTCGTCAAGTGTTGTTCCGAATGAATTATTCCCTAAGCCTATTGTGACTGTCAACATAGAGCCAGAGCCGACGATGGTGCAGCAGGATATCGTTGATATGTACATGAAAAGTATGCAGCAATTTACCGAGTCATTAGCGAAAATGAAGCTTCCAATGGACATTACAAATGAACCAACTAATTCAGGAAATTCAAGCACCGAGCAGAAACTACCGCAATCTAAGAATGCCAACTCCCGTGTCTATTACGGGAGCAGAGCTTTCTTCTGAGTTTTTGCTTCCTTGGTTTAAGGAATACGGGCAGGTGACTGTAGGTTTTAGATTAAAAAAAGGGAATTTGGATTCTCTGCAGTCACACAGTCACGAATCTATGTCGTTAGATGAAGACCGGATCATATTTTAAAATCAATTCAGTGAATTCGAAATACAATCGTTCTGATCTTCATCCAACAAACAGATCCCTTGTGTGTGCGACTATGTGCTTTCGTTGACTGTAGGGAATTCGAGTTCTGAGTCCAGAAAACTGATAATAGCAGTGAAGTGAAATAATGTAGTATCTCCAGTGTGGTTTCTCCTTTCTGTATTTCTTCTGATGTTGTAGGTTACAGGGAATATGACTTTGTACTCTCACAAGCTTGTTGCTATCTTAACTGTTACATGAACTTTTTCTGTGATTACCT
TARGET=1178,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0408
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.015253
PRIMER_LEFT_SEQUENCE=ATCATAGTTCTGAAGCCGGTGT
PRIMER_RIGHT_SEQUENCE=TTCATTCGGAACAACACTTGAC
PRIMER_LEFT=1020,22
PRIMER_RIGHT=1294,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.0521
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.026532
PRIMER_LEFT_1_SEQUENCE=ATCATAGTTCTGAAGCCGGTGT
PRIMER_RIGHT_1_SEQUENCE=TCTCACCATCTTTGACATCCAC
PRIMER_LEFT_1=1020,22
PRIMER_RIGHT_1=1265,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=59.973
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=0.1366
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=0.111077
PRIMER_LEFT_2_SEQUENCE=ATCATAGTTCTGAAGCCGGTGT
PRIMER_RIGHT_2_SEQUENCE=CTCTCACCATCTTTGACATCCA
PRIMER_LEFT_2=1020,22
PRIMER_RIGHT_2=1266,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.1443
PRIMER_LEFT_3_PENALTY=0.025566
PRIMER_RIGHT_3_PENALTY=0.118777
PRIMER_LEFT_3_SEQUENCE=ATCATAGTTCTGAAGCCGGTGT
PRIMER_RIGHT_3_SEQUENCE=TTGACGAATCACTCTCACCATC
PRIMER_LEFT_3=1020,22
PRIMER_RIGHT_3=1277,22
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=60.119
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=0.1624
PRIMER_LEFT_4_PENALTY=0.025566
PRIMER_RIGHT_4_PENALTY=0.136881
PRIMER_LEFT_4_SEQUENCE=ATCATAGTTCTGAAGCCGGTGT
PRIMER_RIGHT_4_SEQUENCE=CCTCTTTGGTTCCTGAAACTTG
PRIMER_LEFT_4=1020,22
PRIMER_RIGHT_4=1235,22
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=60.137
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_03156 Ps_contig_mira-and-tgicl-ass_franssen_28895
SEQUENCE=AAAAAAGAAAACAAAGTAGGTAATACAAACGACAGAGTACTCGGGGACGGGTACTTATTCATTCATTCAGATCATCTTTCTACGCGCAATTATCATGACTAGTTCTTCAGCTGCTTCTCGCAAGATTCTCAGTAAGATTGCCTGCAATAGGCTCCAGAAAGAACTCGTCGAGTGGCAGGTTAATCCACCTACTGGCTTCAAACACAGAGTCACCGATAATCTCCAAAGGTGGGTTATTGAAGTGGCGGGTGCTCCTGGGACACTTTACACCAATGAGACCTATCAGCTTCAGGTTGATTTTCCTGAGAATTACCCTATGGAAGCTCCACAGGTCATATTCTTGCATCCGGCTCCGTTGCATCCTCATATCTACAGCAATGGACATATTTGTTTAGATATATTATATGATTCATGGTCTCCTGCAATGACTGTTAGTTCTATTTGCATTAGCATTCTGTCTATGCTTTCAAGCTCACCTGCAAAGCAACGCCCTGAAGATAATGACCGCTATGTAAAGAACTGCAGAAATGGGCGATCACCCAAGGAGACAAGGTGGTGGTTCCATGATGACAAAGTATGATGACCGGTTTTCGCGAATAAGTTCACAAAAGTGAAATAGAGGATTATCCTTCTGAAAGAAATGCAAAGTCGTGGGAAAAAAACATAACGAGAAACCAGGATCACTTTAATTAAATGGATAATTATTCTCCTGTAAATTAAAATTGCCTGGTAATTTGTAAAGGAAGAGGAAATCAGGAAATTCTGTGAGCAACTTTGATGATCAAACAATTCAGAACAATGTTGTTAATCTCAATGCTATTTGAATATGGATATTGATGGCTGATTAACCCTCATTTGGTCTATATATGTCCTAATTGGACAATATACATGCATAATATTTGTAGGTTTAAA
TARGET=57,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1218
PRIMER_LEFT_PENALTY=1.103921
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=AGTACTCGGGGACGGGTACTTA
PRIMER_RIGHT_SEQUENCE=TTGCAGGCAATCTTACTGAGAA
PRIMER_LEFT=35,22
PRIMER_RIGHT=146,22
PRIMER_LEFT_TM=61.104
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=112
PRIMER_PAIR_PENALTY_1=1.1228
PRIMER_LEFT_1_PENALTY=1.103921
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=AGTACTCGGGGACGGGTACTTA
PRIMER_RIGHT_1_SEQUENCE=GACGAGTTCTTTCTGGAGCCTA
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=168,22
PRIMER_LEFT_1_TM=61.104
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=1.1507
PRIMER_LEFT_2_PENALTY=1.103921
PRIMER_RIGHT_2_PENALTY=0.046801
PRIMER_LEFT_2_SEQUENCE=AGTACTCGGGGACGGGTACTTA
PRIMER_RIGHT_2_SEQUENCE=TGACTCTGTGTTTGAAGCCAGT
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=61.104
PRIMER_RIGHT_2_TM=59.953
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=1.2126
PRIMER_LEFT_3_PENALTY=1.103921
PRIMER_RIGHT_3_PENALTY=0.108684
PRIMER_LEFT_3_SEQUENCE=AGTACTCGGGGACGGGTACTTA
PRIMER_RIGHT_3_SEQUENCE=TCATTGGTGTAAAGTGTCCCAG
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=275,22
PRIMER_LEFT_3_TM=61.104
PRIMER_RIGHT_3_TM=59.891
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=1.2126
PRIMER_LEFT_4_PENALTY=1.103921
PRIMER_RIGHT_4_PENALTY=0.108684
PRIMER_LEFT_4_SEQUENCE=AGTACTCGGGGACGGGTACTTA
PRIMER_RIGHT_4_SEQUENCE=CTCATTGGTGTAAAGTGTCCCA
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=276,22
PRIMER_LEFT_4_TM=61.104
PRIMER_RIGHT_4_TM=59.891
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_03157 Ps_contig_mira-and-tgicl-ass_franssen_28898
SEQUENCE=AGTTTTAAACTTAGTAGATCTGTATTATTTCGGGTCCCTACATAGAAACTTCATACATACATATCTAAAACTAGCATCACCCACCAACTAATGTCCCCTCCACTAAACTAACTTAACTACTTTCAAAATCTGTTCAAAAGTCCCTCTATCGCATGAAACACTGTTTTCAGATTCAAAACGAAATGAAAATGAAATTTAACCATTGCATAACCAAAAATAAAACATTATAACAACATTTTTTTAAAAAAATTTAAACTTTTTTTTTCTTCTTTTTCGAAGATTCCAACTAAGCCTGGAATTCAGGACTGCAGAAGTTTCAGATTTTTTTTTCTATCCCTTTCGTTCCCTCCATTTCTTGGAAATGAATTTCATTCACCGATCCCAACCAACACTGTCTCTAACGATTCTTCTTTGTACATTAATCAAAATTAATTATAAAAAAAGGAATAAAAACGAAAAGGGTTCATCTTTTTTCAAATCTGAAAAAACCATAATTTTCTGATTTTGCTCATCCATCCCTTGTTGGATTCCATTCTCTGAAAATATTTACGAATCGATCCTGCTAACTCCCATTCGCTTCACAGAAGCATTCCTGAATCCATCATCAGTAATACACATTCCACTCTGCTGCTCCAATCCTGCCATGTAGCTTCTCACCTCCGTCCTTATCATCTCCTGCATCACAGTCAATAACTCCGCGCTAAAATTGAACGGAACAACCGATCCTCTTTGTGTCTGCATCGGAACCGCCATCGGAATTGGCGCTGTCATTATCGGAACCAAAGGAATCGCACATGGCGGTGGAGGAGGCTGCGGTAAACCAGTAACAGGCTCTGTTACACGAACTGAATCTTCAGAAGAAGAATCTATACCAGGAAGTGATAATGAAAGTGAAGTTGGAGGATCATTGGATTTAGAAGACGACGTCGTTATCGTTTCTACGAGAGGTAGAGCCGCGACAGTTCTTGTAGGCACGGGGCGGTAAATATGAGAAGAGTTCACGATAGGTACACTGGACTCGCTCACATCGGATCCAGAAGGACTCCCCGGAGTGGGAGGGTTCTTGTAAATCCCAGTAGAAACAGGTATCGCAGCACCAGCACTTACAGATCTTTTCAAAGGTGGAGATTCATCTATCATAATAGAAGAACACTTGCGTTTCAAAGTAGAGTTCCAGTGGTTTTTAATAGCGTTGTCGGTTCGACCCGACAGAAGACGGGCTATGGTAGCCCATTTGTTTCCGAATCGGGCATGTGCTCTGATTATAGTATCATCCTCTTCCGACGTGAAAGCCCGATGCTCCACCTGAGGAGAAAGCTGATTACACCAACGCAGCCTGCAGGATTTCCCGGACCGTCCAGGAATCGATTTGCTGATTATCGACCAGTTTCTCGGCCCGTGCTTCTCAACAAGCTTTTGAAGAGCTTCGTCTTCTTCAGGACTCCATGGACCTTTGATCCGATCCATTTCTTTTCTGGTTATTGCCGTGTTCATCTTTCTTTTGGTATAAAGGTTTTCACAGGGTCATAAATGTAATTAACAGTTGAAATGGTGAAAGAGGGAATAGGAGGTAGAAGAAGAAAAGAGAAGGAGCATTCNNTCGCYNGAGGATGGTGAGGCGGTTTATTTGAGAGAGAAAGGGTAAAAGGAAACCGGTTGGGTAGGTTAGGTTTTGGAGTTGTCAGCGAGCGTTATGGG
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03158 Ps_contig_mira-and-tgicl-ass_franssen_28904
SEQUENCE=TCGGAACGCTTACAAAAAGCCAGAGATTCCTCTGTAGCTATCTTTCAACAAAACCGCGTTCTTCTTTTTCCTTCAAAGCTTTTCATTATCATTATGTCTTCACTTTCAGATCTCATCAATTTTAATCTTTCAGATTCCACAGAAAAGATTATTGCTGAATACATATGGGTTGGTGGATCAGGCATAGACATTAGAAGCAAAGCCAGGACTCTTCCTGGACCAGTGAGTGACCCTGCAAAACTTCCTAAGTGGAACTATGATGGGTCTAGCACAAATCAAGCACCAGGAAAAGATAGTGAAGTTATTTTATATCCACAAGCTATTTTCAAGGACCCATTCAGAAGAGGGAATAATATTCTTGTGATTTGTGATGTTTACACCCCAGCTGGAGAACCATTACCCACAAACAAGAGATACAATGCTGCCAAAATTTTCAGTCACCCTGATGTTGCTGCTGAAGTACCATGGTATGGTATTGAACAAGAGTATACTTTATTACAGAAAGACATCAACTGGCCACTAGGTTGGCCTATTGGTGGATATCCAGGAAAACAGGGTCCATATTATTGTGGAATTGGTGCTGATAAAGCTTATGGACGTGACATTGTTGATGCACATTACAAAGCATGCCTTTTTGCTGGCATTAACATCAGTGGCATCAATGGAGAGGTTATGCCCGGTCAGTGGGAATTTCAAGTTGGCCCTTCTGTTGGTATCTCTGCTGGAGATGAGATATGGGCTGCTCGTTACATTCTAGAGAGGATTACAGAGATTGCTGGAGTTGTTGTTTCATTTGACCCAAAGCCTATTCCGGGTGATTGGAATGGAGCTGGGGCTCATGCAAATTTCAGCACCAAATCAATGAGAGAAAATGGAGGCTACGAGGTAATCAAGAAGGCGATTGAAAAGCTCGGATTGAGGCATAAGGAACACATTGCAGCTTATGGAGAAGGAAATGAGAGACGTCTCACCGGAAAACATGAAACCGCCGATATCAATGTCTTCTCTTGGGGTGTGGCAAACCGTGGAAGCTCAATTAGGGTTGGAAGAGACACAGAAAAAGATGGAAAAGGTTACTTTGAGGATAGAAGGCCTGCATCTAACATGGATCCTTATGTGGTCACTTCCATGATTGCTGAAACCACCATTCTCTGGAAGAAACCATGAAGTTCCAACCACCACAAGTCTTTGGATTTACACCACTACGTATAATGAGTCCAATGCCATTTATATATATCTACTTTATATCTATTATTATTATTATTAAAATAATAATATCTATTATTATGCAAATGTGTTACTTCATTAGAAGTAATTTTCAAGTGGGGTCTTTAAGCAAAGAGTATATGGTCCATGTATTTGGATTACAATAATTTTATCATCAATAACATGTGGTGCTTCTTCNTATTTTTTCTTCTTTGAATGTATGGATATCAATTAATGACTAATTAGTTTGTTTGTTTTGTAAACT
TARGET=1250,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1597
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.155310
PRIMER_LEFT_SEQUENCE=ATTGCTGAAACCACCATTCTCT
PRIMER_RIGHT_SEQUENCE=CTTTGCTTAAAGACCCCACTTG
PRIMER_LEFT=1131,22
PRIMER_RIGHT=1340,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.155
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.1789
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.155310
PRIMER_LEFT_1_SEQUENCE=CTGGAAGAAACCATGAAGTTCC
PRIMER_RIGHT_1_SEQUENCE=CTTTGCTTAAAGACCCCACTTG
PRIMER_LEFT_1=1151,22
PRIMER_RIGHT_1=1340,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.155
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.1789
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.155310
PRIMER_LEFT_2_SEQUENCE=AACCACCATTCTCTGGAAGAAA
PRIMER_RIGHT_2_SEQUENCE=CTTTGCTTAAAGACCCCACTTG
PRIMER_LEFT_2=1139,22
PRIMER_RIGHT_2=1340,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.155
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.2173
PRIMER_LEFT_3_PENALTY=0.061961
PRIMER_RIGHT_3_PENALTY=0.155310
PRIMER_LEFT_3_SEQUENCE=AAGTTCCAACCACCACAAGTCT
PRIMER_RIGHT_3_SEQUENCE=CTTTGCTTAAAGACCCCACTTG
PRIMER_LEFT_3=1166,22
PRIMER_RIGHT_3=1340,22
PRIMER_LEFT_3_TM=59.938
PRIMER_RIGHT_3_TM=60.155
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.2731
PRIMER_LEFT_4_PENALTY=0.117817
PRIMER_RIGHT_4_PENALTY=0.155310
PRIMER_LEFT_4_SEQUENCE=TCACTTCCATGATTGCTGAAAC
PRIMER_RIGHT_4_SEQUENCE=CTTTGCTTAAAGACCCCACTTG
PRIMER_LEFT_4=1120,22
PRIMER_RIGHT_4=1340,22
PRIMER_LEFT_4_TM=60.118
PRIMER_RIGHT_4_TM=60.155
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_03159 Ps_contig_mira-and-tgicl-ass_franssen_28904
SEQUENCE=TCGGAACGCTTACAAAAAGCCAGAGATTCCTCTGTAGCTATCTTTCAACAAAACCGCGTTCTTCTTTTTCCTTCAAAGCTTTTCATTATCATTATGTCTTCACTTTCAGATCTCATCAATTTTAATCTTTCAGATTCCACAGAAAAGATTATTGCTGAATACATATGGGTTGGTGGATCAGGCATAGACATTAGAAGCAAAGCCAGGACTCTTCCTGGACCAGTGAGTGACCCTGCAAAACTTCCTAAGTGGAACTATGATGGGTCTAGCACAAATCAAGCACCAGGAAAAGATAGTGAAGTTATTTTATATCCACAAGCTATTTTCAAGGACCCATTCAGAAGAGGGAATAATATTCTTGTGATTTGTGATGTTTACACCCCAGCTGGAGAACCATTACCCACAAACAAGAGATACAATGCTGCCAAAATTTTCAGTCACCCTGATGTTGCTGCTGAAGTACCATGGTATGGTATTGAACAAGAGTATACTTTATTACAGAAAGACATCAACTGGCCACTAGGTTGGCCTATTGGTGGATATCCAGGAAAACAGGGTCCATATTATTGTGGAATTGGTGCTGATAAAGCTTATGGACGTGACATTGTTGATGCACATTACAAAGCATGCCTTTTTGCTGGCATTAACATCAGTGGCATCAATGGAGAGGTTATGCCCGGTCAGTGGGAATTTCAAGTTGGCCCTTCTGTTGGTATCTCTGCTGGAGATGAGATATGGGCTGCTCGTTACATTCTAGAGAGGATTACAGAGATTGCTGGAGTTGTTGTTTCATTTGACCCAAAGCCTATTCCGGGTGATTGGAATGGAGCTGGGGCTCATGCAAATTTCAGCACCAAATCAATGAGAGAAAATGGAGGCTACGAGGTAATCAAGAAGGCGATTGAAAAGCTCGGATTGAGGCATAAGGAACACATTGCAGCTTATGGAGAAGGAAATGAGAGACGTCTCACCGGAAAACATGAAACCGCCGATATCAATGTCTTCTCTTGGGGTGTGGCAAACCGTGGAAGCTCAATTAGGGTTGGAAGAGACACAGAAAAAGATGGAAAAGGTTACTTTGAGGATAGAAGGCCTGCATCTAACATGGATCCTTATGTGGTCACTTCCATGATTGCTGAAACCACCATTCTCTGGAAGAAACCATGAAGTTCCAACCACCACAAGTCTTTGGATTTACACCACTACGTATAATGAGTCCAATGCCATTTATATATATCTACTTTATATCTATTATTATTATTATTAAAATAATAATATCTATTATTATGCAAATGTGTTACTTCATTAGAAGTAATTTTCAAGTGGGGTCTTTAAGCAAAGAGTATATGGTCCATGTATTTGGATTACAATAATTTTATCATCAATAACATGTGGTGCTTCTTCNTATTTTTTCTTCTTTGAATGTATGGATATCAATTAATGACTAATTAGTTTGTTTGTTTTGTAAACT
TARGET=1452,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03160 Ps_contig_mira-and-tgicl-ass_franssen_28905
SEQUENCE=TTAGATTTAGTGTTGTCTCCAACATAAATAACTCCAACGTTCTGAATTTCAAAATTTCAAATTTTAATTGAGATGTAAAATAAAAATTTAAAAAATATATATATATTCATTATTATTTAAACTAGAGTTCTTCTAAATGTTCCGTCTTTATCTTAAAAACAGAATAATTTCCTCATAGAGAATTTGAATTATCTTCAAATATTTCTATACCATAAATTGAAGAACATTTAAATGGTCCAGCTTTCCCTAAAAGAACACTTCATTGAGTACAAAACAACAAACATTGTATTGATCCATACATTCAAAGANNNNNATAGAAGAAGCACCACATGTTATTGATGATAAAATTATTGTACTCCAAATACATGGACCATATATCCTTTGCTTAAAGACCACTTGAAAATTACTTCTAATGAAGTAACACATTTGCATAATAAATAGATATTATTATNTTAATAATAATAATAATAATAGATAATAAACTAGATATATATAAATGGATAATAAACTAGATATATATAAATGGCATTGGACTCATTANACGTAGTGGTGTAAATCCAAAGACTTGTGGTGGTTGGAACTTCATGATTTCTTCCAGAGAATGGTGGTTTCAGCAATCATGGAAGTGACCACATAAGGATCCATGTTAGATGCAGGCCTTCTTATCCTTCAAAGTAACCTTTTCCATCTTTTTCTGTGTCTCTTCCAACCCTAATTGAGCTTCCACGGTTTGCCACACCCTACACCAAAGAAAAAATCTTCAAGTTAGATATTGCAAATTAAAAATATTATGGTACAAAATTTCTTTGACTACTCAAACATAATGCAAATAGATTTTGAAGTTAGGGTTTCTATTTCGACTTACCCAAGAGAAGACATTGATATCGGCGGTTTCATGTTTTCCGGTGAGACGTCTCTCATTTCCTTCTCCATAAGCTGCAATGTGTTCCTTATGCCTCAATCCGAGCTTTTCAATCGCCTTCTTGATTACCTCGTAGCCTCCATTTTCTCTCATTGATTTGGTGCTGAAATTTGCATGACGCCCCAGCCTCCATTCCAATCCACCGGAATGGCTTTGGGACAAATAGAAAACCAACAACCTCC
TARGET=95,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.3991
PRIMER_LEFT_PENALTY=3.169620
PRIMER_RIGHT_PENALTY=0.229480
PRIMER_LEFT_SEQUENCE=GTCTCCAACATAAATAACTCCAACG
PRIMER_RIGHT_SEQUENCE=TGTTCTTTTAGGGAAAGCTGGA
PRIMER_LEFT=14,25
PRIMER_RIGHT=256,22
PRIMER_LEFT_TM=60.170
PRIMER_RIGHT_TM=60.229
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=3.8658
PRIMER_LEFT_1_PENALTY=3.169620
PRIMER_RIGHT_1_PENALTY=0.696189
PRIMER_LEFT_1_SEQUENCE=GTCTCCAACATAAATAACTCCAACG
PRIMER_RIGHT_1_SEQUENCE=GTGTTCTTTTAGGGAAAGCTGG
PRIMER_LEFT_1=14,25
PRIMER_RIGHT_1=257,22
PRIMER_LEFT_1_TM=60.170
PRIMER_RIGHT_1_TM=59.304
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=3.9765
PRIMER_LEFT_2_PENALTY=3.169620
PRIMER_RIGHT_2_PENALTY=0.806834
PRIMER_LEFT_2_SEQUENCE=GTCTCCAACATAAATAACTCCAACG
PRIMER_RIGHT_2_SEQUENCE=CTTTTAGGGAAAGCTGGACCAT
PRIMER_LEFT_2=14,25
PRIMER_RIGHT_2=252,22
PRIMER_LEFT_2_TM=60.170
PRIMER_RIGHT_2_TM=60.807
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=4.0797
PRIMER_LEFT_3_PENALTY=3.169620
PRIMER_RIGHT_3_PENALTY=0.910121
PRIMER_LEFT_3_SEQUENCE=GTCTCCAACATAAATAACTCCAACG
PRIMER_RIGHT_3_SEQUENCE=TTCTTTTAGGGAAAGCTGGACC
PRIMER_LEFT_3=14,25
PRIMER_RIGHT_3=254,22
PRIMER_LEFT_3_TM=60.170
PRIMER_RIGHT_3_TM=60.910
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=4.2173
PRIMER_LEFT_4_PENALTY=3.169620
PRIMER_RIGHT_4_PENALTY=1.047652
PRIMER_LEFT_4_SEQUENCE=GTCTCCAACATAAATAACTCCAACG
PRIMER_RIGHT_4_SEQUENCE=TTTAGGGAAAGCTGGACCATT
PRIMER_LEFT_4=14,25
PRIMER_RIGHT_4=250,21
PRIMER_LEFT_4_TM=60.170
PRIMER_RIGHT_4_TM=59.952
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_03161 Ps_contig_mira-and-tgicl-ass_franssen_28905
SEQUENCE=TTAGATTTAGTGTTGTCTCCAACATAAATAACTCCAACGTTCTGAATTTCAAAATTTCAAATTTTAATTGAGATGTAAAATAAAAATTTAAAAAATATATATATATTCATTATTATTTAAACTAGAGTTCTTCTAAATGTTCCGTCTTTATCTTAAAAACAGAATAATTTCCTCATAGAGAATTTGAATTATCTTCAAATATTTCTATACCATAAATTGAAGAACATTTAAATGGTCCAGCTTTCCCTAAAAGAACACTTCATTGAGTACAAAACAACAAACATTGTATTGATCCATACATTCAAAGANNNNNATAGAAGAAGCACCACATGTTATTGATGATAAAATTATTGTACTCCAAATACATGGACCATATATCCTTTGCTTAAAGACCACTTGAAAATTACTTCTAATGAAGTAACACATTTGCATAATAAATAGATATTATTATNTTAATAATAATAATAATAATAGATAATAAACTAGATATATATAAATGGATAATAAACTAGATATATATAAATGGCATTGGACTCATTANACGTAGTGGTGTAAATCCAAAGACTTGTGGTGGTTGGAACTTCATGATTTCTTCCAGAGAATGGTGGTTTCAGCAATCATGGAAGTGACCACATAAGGATCCATGTTAGATGCAGGCCTTCTTATCCTTCAAAGTAACCTTTTCCATCTTTTTCTGTGTCTCTTCCAACCCTAATTGAGCTTCCACGGTTTGCCACACCCTACACCAAAGAAAAAATCTTCAAGTTAGATATTGCAAATTAAAAATATTATGGTACAAAATTTCTTTGACTACTCAAACATAATGCAAATAGATTTTGAAGTTAGGGTTTCTATTTCGACTTACCCAAGAGAAGACATTGATATCGGCGGTTTCATGTTTTCCGGTGAGACGTCTCTCATTTCCTTCTCCATAAGCTGCAATGTGTTCCTTATGCCTCAATCCGAGCTTTTCAATCGCCTTCTTGATTACCTCGTAGCCTCCATTTTCTCTCATTGATTTGGTGCTGAAATTTGCATGACGCCCCAGCCTCCATTCCAATCCACCGGAATGGCTTTGGGACAAATAGAAAACCAACAACCTCC
TARGET=454,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4422
PRIMER_LEFT_PENALTY=1.437826
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=CCTTTGCTTAAAGACCACTTGA
PRIMER_RIGHT_SEQUENCE=ATTGCTGAAACCACCATTCTCT
PRIMER_LEFT=378,22
PRIMER_RIGHT=617,22
PRIMER_LEFT_TM=58.562
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=1.4422
PRIMER_LEFT_1_PENALTY=1.437826
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=TCCTTTGCTTAAAGACCACTTG
PRIMER_RIGHT_1_SEQUENCE=ATTGCTGAAACCACCATTCTCT
PRIMER_LEFT_1=377,22
PRIMER_RIGHT_1=617,22
PRIMER_LEFT_1_TM=58.562
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=1.4614
PRIMER_LEFT_2_PENALTY=1.437826
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=CCTTTGCTTAAAGACCACTTGA
PRIMER_RIGHT_2_SEQUENCE=AACCACCATTCTCTGGAAGAAA
PRIMER_LEFT_2=378,22
PRIMER_RIGHT_2=609,22
PRIMER_LEFT_2_TM=58.562
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=1.4614
PRIMER_LEFT_3_PENALTY=1.437826
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=TCCTTTGCTTAAAGACCACTTG
PRIMER_RIGHT_3_SEQUENCE=AACCACCATTCTCTGGAAGAAA
PRIMER_LEFT_3=377,22
PRIMER_RIGHT_3=609,22
PRIMER_LEFT_3_TM=58.562
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=1.4977
PRIMER_LEFT_4_PENALTY=1.437826
PRIMER_RIGHT_4_PENALTY=0.059875
PRIMER_LEFT_4_SEQUENCE=TCCTTTGCTTAAAGACCACTTG
PRIMER_RIGHT_4_SEQUENCE=CCACCATTCTCTGGAAGAAATC
PRIMER_LEFT_4=377,22
PRIMER_RIGHT_4=607,22
PRIMER_LEFT_4_TM=58.562
PRIMER_RIGHT_4_TM=59.940
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_03162 Ps_contig_mira-and-tgicl-ass_franssen_28906
SEQUENCE=AGATAAACAAAACAAAATATTGTAGAACTATTGCATGGCAAAAGATAATGAACATTTGACATACACAATTATAAATGCATAGTTGCTGCAACAAAATTAGACTCATGCCAATGACCATTTCACAAGTTTGAATGATGGAACTATATCACACAGGAAATAAAAGAACAATTAAGAGTGGAGGGCAATGTAACAAAGCATTTACCCTCTATTGCTTGAAATAATAAAACTGAAATAAGAACTTCTTGCACTCGTATAATAAGAGATCAATAATCATCTGGTTCCTCTTCATCTTCTGCTCCCTCTGCACCAACCTCTTCGTAATCTTTCTCAAGAGCAGCCAAATCTTCACGGGCTTCCGAGAACTCTCCTTCTTCCATTCCTTCACCAACATACCAATGGACAAAGGCCCTCTTTGCATACATAAGATCAAACTTATGGTCAATGCGGGAGAAGACCTCTGCAACAGCAGTGTTGTTACTGATCATACAGACAGCTCTTTGCACCTTAGCAAGGTCGCCGCCTGGCACTACTGAAGGAGGCTGATAGTTGATACCACATTTGAAACCAGTTGGGCACCAATCAACAAATTGAACAGTCCTTTTGGTTTTGATGGTGCCAACAGCAGCATTGACATCCTTTGGAACAACATCTCCTCGGTACATCAAACAACAAGCCATGTATTTTCCATGTCTTGGGTCACACTTGGCCATCATACTAGCAGGCTCGAACACAGCATTAGTAATCTCAGGCACAGAGAGCTGCTCATGGTAAGCCTTAGCAGCAGAGATAACAGGGGCATATGACGAAAGCATGAAGTGGATACGAGGATAAGGCACAAGATTGGTCTGGAACTCAGTAATATCAACATTAATAGCACCATCAAACCTCAACGAGGTAGTGAGGGATGATATAATCTGTGATATCAACCTGTTCAAGTTGGTGTAAGTAGGCCTTTCAATATCAAGAGATCTCCTGCAAATGTCATAGATCGCCTCATTGTCCAAGAGCACAGCCACATCAGTGTGCTCAAGGAGAGAATGTGTGGAGAGAACACTGTTGTATGGTTCAACAACAGCAGTTGAAACCTGAGGGGAAGGGTAGATGGTGAAACCAAGTTTGGACTTCTTTCCATAATCAACAGAGAGACGCTCCAACAAGAGTGAACCCAAACCAGAGCCGGTTCCACCACCAACAGCGTTGAAGACCAAAAATCCTTGAAGACCGGTACAGTTATCAGCCAACTTCCTGACACGATCTAAGCACAGATCTACGATTTCCTTGCCAACAGTGTAATGTCCTCTGGCGAAATTGTTAGCAGCATCTTCCTTGCCGGAGATGAGTTGCTCCGGGTGAAAGAGTTGCCGGTAGGTACCGGACCTGACTTCATCGATGACGGTAGGTTCTAGATCTACAAAAACGGCACGAGGAACGTGCTTGCCGGAACCGGTTTCGCTGAAGAAAGTGTTGAATGCATCGTGCGCAACTCCGACGGAGGAATCACTTGGCATCATGCCGTCGGGCTGAATGCCATGTTCGAGACAGTAAAGCTCCCAGCAGGAGTTTCCGACTTGAATACCGGCCTGACCGATGTGAATGCTGATAATTTCTCTCATCTTTTCTCGATCTAGATTTGAAAGTTTTGAAAGAAAGAAGAGGGAGAGGAAAAGGTGGTTATGGAAAGAGAGAGAGGAAATGAGAGGGTTTCGCGTCGTGTGTTGTGACTCGCTAGTATTAGTAGTCCNNGTGTTTAGTGTGTCGAATCAATAATACTAGTGCACATCCCCC
TARGET=1680,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0673
PRIMER_LEFT_PENALTY=0.139753
PRIMER_RIGHT_PENALTY=0.927507
PRIMER_LEFT_SEQUENCE=GAATGCCATGTTCGAGACAGTA
PRIMER_RIGHT_SEQUENCE=TACTAGCGAGTCACAACACACG
PRIMER_LEFT=1522,22
PRIMER_RIGHT=1730,22
PRIMER_LEFT_TM=60.140
PRIMER_RIGHT_TM=59.072
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=1.0864
PRIMER_LEFT_1_PENALTY=0.139753
PRIMER_RIGHT_1_PENALTY=0.946622
PRIMER_LEFT_1_SEQUENCE=GAATGCCATGTTCGAGACAGTA
PRIMER_RIGHT_1_SEQUENCE=ACTAGCGAGTCACAACACACGA
PRIMER_LEFT_1=1522,22
PRIMER_RIGHT_1=1729,22
PRIMER_LEFT_1_TM=60.140
PRIMER_RIGHT_1_TM=60.947
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=1.0864
PRIMER_LEFT_2_PENALTY=0.139753
PRIMER_RIGHT_2_PENALTY=0.946622
PRIMER_LEFT_2_SEQUENCE=GAATGCCATGTTCGAGACAGTA
PRIMER_RIGHT_2_SEQUENCE=CTAGCGAGTCACAACACACGAC
PRIMER_LEFT_2=1522,22
PRIMER_RIGHT_2=1728,22
PRIMER_LEFT_2_TM=60.140
PRIMER_RIGHT_2_TM=60.947
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=1.1751
PRIMER_LEFT_3_PENALTY=0.247631
PRIMER_RIGHT_3_PENALTY=0.927507
PRIMER_LEFT_3_SEQUENCE=GAAGAAAGTGTTGAATGCATCG
PRIMER_RIGHT_3_SEQUENCE=TACTAGCGAGTCACAACACACG
PRIMER_LEFT_3=1454,22
PRIMER_RIGHT_3=1730,22
PRIMER_LEFT_3_TM=59.752
PRIMER_RIGHT_3_TM=59.072
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=1.1782
PRIMER_LEFT_4_PENALTY=0.139753
PRIMER_RIGHT_4_PENALTY=1.038444
PRIMER_LEFT_4_SEQUENCE=GAATGCCATGTTCGAGACAGTA
PRIMER_RIGHT_4_SEQUENCE=TAGCGAGTCACAACACACGAC
PRIMER_LEFT_4=1522,22
PRIMER_RIGHT_4=1727,21
PRIMER_LEFT_4_TM=60.140
PRIMER_RIGHT_4_TM=59.962
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=206
=
PRIMER_SEQUENCE_ID=SSR_03163 Ps_contig_mira-and-tgicl-ass_franssen_28909
SEQUENCE=AAGACAGTGGTTAGTACAACGACAGAGTTACTTTTTTTTTTAGTTTTTTTTTTTTTTTTTTTTAAAATTTTTAAACTTATAATCCACTGTCATTTATAAGTTTCTTATAAATAAAAAGGGCAAAAGGTTCTGAAAACAATACCCAACCAATTTAGAACAAACAAACAGAAAAACTACCAAAAATTCAAAATCTAACACCCAAACAAAACCCTAAAAATCTCTTAAATTATATCAGTCAGCCTTAGAAGCAGTAGCGGCAGCCTGCCCCCTAAGTCGACCAGTTCTCTTAGCAGCAATAAGACCAACCTTCTGCCCGGGAGGAGCATCACGTCTAACAGTACTAGCATGTCCAATATGTTGGTGGTTACCTCCTCCGTGGGGATGCTCAACTGGATTCATAGCCACACCACGAACCTTAGGCCAGCAGTTCCTCTTCACCCTAAACTTGTGGTATGCATT
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8204
PRIMER_LEFT_PENALTY=1.820332
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=GGTTCTGAAAACAATACCCAACC
PRIMER_RIGHT_SEQUENCE=GTGTGGCTATGAATCCAGTTGA
PRIMER_LEFT=125,23
PRIMER_RIGHT=406,22
PRIMER_LEFT_TM=60.820
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=1.9298
PRIMER_LEFT_1_PENALTY=1.820332
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=GGTTCTGAAAACAATACCCAACC
PRIMER_RIGHT_1_SEQUENCE=GGAGGAGGTAACCACCAACATA
PRIMER_LEFT_1=125,23
PRIMER_RIGHT_1=374,22
PRIMER_LEFT_1_TM=60.820
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=1.9526
PRIMER_LEFT_2_PENALTY=1.820332
PRIMER_RIGHT_2_PENALTY=0.132262
PRIMER_LEFT_2_SEQUENCE=GGTTCTGAAAACAATACCCAACC
PRIMER_RIGHT_2_SEQUENCE=TGTGGCTATGAATCCAGTTGAG
PRIMER_LEFT_2=125,23
PRIMER_RIGHT_2=405,22
PRIMER_LEFT_2_TM=60.820
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=281
PRIMER_PAIR_PENALTY_3=2.1608
PRIMER_LEFT_3_PENALTY=2.160811
PRIMER_RIGHT_3_PENALTY=0.000037
PRIMER_LEFT_3_SEQUENCE=AGGGCAAAAGGTTCTGAAAACA
PRIMER_RIGHT_3_SEQUENCE=GTGTGGCTATGAATCCAGTTGA
PRIMER_LEFT_3=116,22
PRIMER_RIGHT_3=406,22
PRIMER_LEFT_3_TM=62.161
PRIMER_RIGHT_3_TM=60.000
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=2.2703
PRIMER_LEFT_4_PENALTY=2.160811
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=AGGGCAAAAGGTTCTGAAAACA
PRIMER_RIGHT_4_SEQUENCE=GGAGGAGGTAACCACCAACATA
PRIMER_LEFT_4=116,22
PRIMER_RIGHT_4=374,22
PRIMER_LEFT_4_TM=62.161
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=259
=
PRIMER_SEQUENCE_ID=SSR_03164 Ps_contig_mira-and-tgicl-ass_franssen_28926
SEQUENCE=GTCGGGCTGAGTCAAGTTCCAAAGTAGCTCTCACTGATTCCCTTTCGATCTAACCGAATATTCGTTCGCACAACAATGGCGATTAGGGCTCGTCACCCTAACGTTCTCTCTCTCGTTCTTATCTCATTTCTCTCCATAGCTTCTGCTAAGGTTTTCTTCGAAGAACGTTTCGAAGATGGATGGGAAAGTCGTTGGGTAAAATCTGAATGGAAGAAAGATGAGAATTTGGCCGGAGAATGGAACTACACCTCAGGTCAATGGAATGGAGACGCTAATGATAAAGGTATTCAAACAAGTGAAGATTACCGATTCTATGCTATCTCTGCTGAATTCCCTGAATTCACCAACAAGGATAACACCCTAGTATTCCAATTTTCTGTCAAGCACGAACAGAAGCTTGACTGTGGTGGTGGATACTTGAAGTTGCTTAGTGGCGATGTTGATCAGAAGAAATTTGGCGGTGACACTCCTTACAGTATCATGTTTGGTCCAGATATCTGTGGTTACAGTACCAAGAAAGTACATGCTATTTTGACCTACAATGACACAAACCAGTTGATTAAGAAGGATGTTCCATGTGAGACAGACCAGCTTACACATGTTTACACCTTCATCATCCGTCCGGATGCAACCTACAGTATTCTGATCGACAATGTGGAGAAGCAAACTGGTAGCCTCTACACTGATTGGAATCTTCTTCCTGCAAAGAAAATTAAGGATCCTGAGGCCAAGAAGCCAGAAGATTGGGATGATAAGGAGTTTATTCCTGACCCTGAGGATAAGAAACCAGAGGGATACGATGACATTCCTAAAGAGATTCCAGACTCTGATGCCAAAAAGCCTGAAGACTGGGATGATGAGGAGGACGGTGAGTGGACAGCACCTACAATTGCCAACCCAGAGTACAAGGGACCATGGAAGCCAAAGAAAATTAAGAACCCTAACTACAGTGGAAAGTGGAAGGCACCATTGATTGACAACCCAGACTTCAAGGACGACCCAGACCTATATGTTTTCAAGTTGAAGTATGTTGGCATTGAATTGTGGCAGGTAAAATCTGGTACCTTGTTCGACAATGTTGTTATTACTGATGATCCCGAGTATGCCAAACAACTAGCTGAAGAAACATGGGGCAAACAGAAGGATGCTGAGAAGGCAGCATTTGATGAGGCTGAGAAGAAGAAAGAAGAGGAGGAATCAAAGGATGACCCAGTTGACTCTGATGCGGAAGAGGATGATGAAGATACTGATGATGCTGATGATGCTGAATCAAAGACAGAAGCTGCCGAGGACAGCGATGAATCCAGTAAAGAGGAAGTACATGATGAGCTCTAGGGTGTTTGCAAAGCATATGACATATGCTAGAGTTAGGAGAAGTTTTATTCCAGAGTGGAAGTTATCTTTTTTAGCTGTGTAGTATTTCTCTCTTAGATAAAGCAAACCGTTAAAACTGTAAGCAAAAAAGCTTGAGCTTTTTGCTTCATCCCATTAGACTATTCAGCTGATTAGAACAAACAACAATGCTTCATTTCAATTATCGTTTATTATGTTCTGATCGAGTTTGCCTTTTATGGATGCCTCAAAGTCTAATCTTCTAAANTNTTTTGNGAAACC
TARGET=105,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4612
PRIMER_LEFT_PENALTY=0.287182
PRIMER_RIGHT_PENALTY=0.174021
PRIMER_LEFT_SEQUENCE=CTCACTGATTCCCTTTCGATCT
PRIMER_RIGHT_SEQUENCE=ATCATTAGCGTCTCCATTCCAT
PRIMER_LEFT=29,22
PRIMER_RIGHT=278,22
PRIMER_LEFT_TM=59.713
PRIMER_RIGHT_TM=59.826
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=0.4612
PRIMER_LEFT_1_PENALTY=0.287182
PRIMER_RIGHT_1_PENALTY=0.174021
PRIMER_LEFT_1_SEQUENCE=CTCTCACTGATTCCCTTTCGAT
PRIMER_RIGHT_1_SEQUENCE=ATCATTAGCGTCTCCATTCCAT
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=278,22
PRIMER_LEFT_1_TM=59.713
PRIMER_RIGHT_1_TM=59.826
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.3000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.5997
PRIMER_LEFT_2_PENALTY=0.287182
PRIMER_RIGHT_2_PENALTY=0.312541
PRIMER_LEFT_2_SEQUENCE=CTCACTGATTCCCTTTCGATCT
PRIMER_RIGHT_2_SEQUENCE=CGAAGAAAACCTTAGCAGAAGC
PRIMER_LEFT_2=29,22
PRIMER_RIGHT_2=159,22
PRIMER_LEFT_2_TM=59.713
PRIMER_RIGHT_2_TM=59.687
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=131
PRIMER_PAIR_PENALTY_3=0.5997
PRIMER_LEFT_3_PENALTY=0.287182
PRIMER_RIGHT_3_PENALTY=0.312541
PRIMER_LEFT_3_SEQUENCE=CTCTCACTGATTCCCTTTCGAT
PRIMER_RIGHT_3_SEQUENCE=CGAAGAAAACCTTAGCAGAAGC
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=159,22
PRIMER_LEFT_3_TM=59.713
PRIMER_RIGHT_3_TM=59.687
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=0.6890
PRIMER_LEFT_4_PENALTY=0.287182
PRIMER_RIGHT_4_PENALTY=0.401769
PRIMER_LEFT_4_SEQUENCE=CTCACTGATTCCCTTTCGATCT
PRIMER_RIGHT_4_SEQUENCE=ATCCATCTTCGAAACGTTCTTC
PRIMER_LEFT_4=29,22
PRIMER_RIGHT_4=180,22
PRIMER_LEFT_4_TM=59.713
PRIMER_RIGHT_4_TM=59.598
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_03165 Ps_contig_mira-and-tgicl-ass_franssen_28946
SEQUENCE=AAATTAATACATGATTAATTAACCGAACGGAAGAATACAATAAATAGATAAGAACAACAGAATACGNGGAGAGAGAAAAAAAGAAAGAATTACTAGTACAATTTTGATTTTACAGTCTCTAAAAAGTCATCACATCACCATCTTCTTCTTCTTCAATTTCTCTCTGCTCAGAAATGGGGGTATATCTCAGTACTCCCAAAACTGAGAAATTTTCTGAAGATGGAGAAAGTCACAATCTTAGATATGGTTTATCGTCCATGCAAGGCTGGCGTGCAACAATGGAAGATGCTCATGCTGCATATACTGATCTGGATGGTTCTACTTCGTTTTTTGGTGTTTATGATGGTCATGGAGGTAAGGTGGTTGCAAAGTTTTGTGCTAAGTTTCTTCATCAACAGATGCTCAAAAGTGAAGCATACTTAGTTGGAGATATTGGGACTTCTCTTCAAGAATCATTTTTAAGAATGGATGAGATGATGCGTGGTCAAAGAGGATGGAGGGAGCTATCAATCTTGGGAGATAAGATAAACAAATTTAGTGGGATGATAGAAGGGTTGATTTGGTCTCCACGAAGCAGCAATGGTATTAGCAGAGCTGATGATTGGGCTTTTGAGGAGGGGCCTCATTCTGATTTTGCTGGACCAACTTCAGGAAGCACAGCGTGTGTTGCAGTTATCAGAAACAACCAGCTTCTTGTTGGAAATGCTGGTGATTCACGTTGTGTGATATCTCGAAAGGGACAGGCATACAATTTGTCCAGAGACCACAAACCTGATCTTGAGATTGAGAAGGAAAGAATTTCGAAAGCTGGTGGTTTTATTCATGCAGGAAGAGTCAATGGCAGTTTAAACCTTACAAGAGCTATTGGTGACATGGAATTTAAACAGAACAAATTTCTTCCTGCTGAAAAACAAGTTGTAACTGCCAATCCAGATATAAACACCGTTGAGCTTTGTGATGAAGATGAATTTATGGTTCTAGCTTGTGATGGCATATGGGACTGCTTGTCAAGTCAACAATTGGTAGATTTTGTTCATGAACAATTGTGTTCGGAAACAAAACTTTCCACTGTGTGCGAAAGAGTATTTGACCGGTGTTTGGCTCCATCAACTGCTGGTGGCGAAGGATGTGATAACATGACCATGATCTTGGTTCAGTTCAAAAAACCTGCTCAGTCCAGTGCACCAGCAGAAGAGCAATCTTCATCAAACGAACTAGCTGATTCACAAGTTGAACTGAAGCTTGAAAGAAGTGATGTTTAGTACTGTATTCCATAGCAACTGTTCAAATATGCATTCTATATAATACCTAATAGACACAGTTCAAATTTGCATTCTACATATATAATAGGCACACACACTCACCCCCTATGTATAGTAGTAGTGTACACAAGTGCATATTTTGTTTTCTGAATGTCCATTCCCATTTCATCTGAAATTATATGCAGTAGTTATTTTGATCACTATAAATAATATCAGAGTTGTTCTAGTTTCAACTAANAAACCG
TARGET=141,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2075
PRIMER_LEFT_PENALTY=1.060824
PRIMER_RIGHT_PENALTY=0.146683
PRIMER_LEFT_SEQUENCE=GAACAACAGAATACGNGGAGAGA
PRIMER_RIGHT_SEQUENCE=CTTGCATGGACGATAAACCATA
PRIMER_LEFT=51,23
PRIMER_RIGHT=263,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=59.853
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=1.2315
PRIMER_LEFT_1_PENALTY=1.060824
PRIMER_RIGHT_1_PENALTY=0.170703
PRIMER_LEFT_1_SEQUENCE=GAACAACAGAATACGNGGAGAGA
PRIMER_RIGHT_1_SEQUENCE=GATATACCCCCATTTCTGAGCA
PRIMER_LEFT_1=51,23
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=60.171
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.75
PRIMER_PAIR_1_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=1.2708
PRIMER_LEFT_2_PENALTY=1.124156
PRIMER_RIGHT_2_PENALTY=0.146683
PRIMER_LEFT_2_SEQUENCE=CAACAGAATACGNGGAGAGAGA
PRIMER_RIGHT_2_SEQUENCE=CTTGCATGGACGATAAACCATA
PRIMER_LEFT_2=54,22
PRIMER_RIGHT_2=263,22
PRIMER_LEFT_2_TM=58.876
PRIMER_RIGHT_2_TM=59.853
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=1.2949
PRIMER_LEFT_3_PENALTY=1.124156
PRIMER_RIGHT_3_PENALTY=0.170703
PRIMER_LEFT_3_SEQUENCE=CAACAGAATACGNGGAGAGAGA
PRIMER_RIGHT_3_SEQUENCE=GATATACCCCCATTTCTGAGCA
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=58.876
PRIMER_RIGHT_3_TM=60.171
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.75
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=1.3317
PRIMER_LEFT_4_PENALTY=1.185055
PRIMER_RIGHT_4_PENALTY=0.146683
PRIMER_LEFT_4_SEQUENCE=CAACAGAATACGNGGAGAGAGAA
PRIMER_RIGHT_4_SEQUENCE=CTTGCATGGACGATAAACCATA
PRIMER_LEFT_4=54,23
PRIMER_RIGHT_4=263,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=59.853
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_03166 Ps_contig_mira-and-tgicl-ass_franssen_28961
SEQUENCE=ATCAACGCAGAGTACGCGGGGACCGCCGAATCCAAAATAATTAAAAAAAAGAAAAAAAAACCAATAGCGTCCCCTAACCCACCGTGCGAGGTCCCTCGACCTCCGCACAGCCAGGGTATTATTATGTCTGACACTCACGCGCCGTCGTCTCCTCCTCCTTCCTTTCTCTTCCACCGTCCACCTTTGTTCAAACAGGGATTTTCTTCTCCGCTTCTGAAGCAGAAATCGTGGTCGCCGGATCTTTATCGCGACGAGGCCTGGCTTCGACGGAAAGGAAACTGGAAGAATCGGAGAAGCAAAAGCGTGACGGATGAAGATGTTGATGAACTCAAAGCCTGCATTGAGTTAGGATTCGGATTCGAATCTTCTCCAGAAGTTGAAACCGATCGGAGACTTGTAGATACTTTACCAGCTCTTGAACTCTATCACGCCGTCAATAAAAGTTACAACGATTCTCGTAACCCTAAACCTGTCACCACCACCGTAACTACTCCATCTTCCTCCGCTGCATCCGATCGTGACGGCACTCCTTCTCCTCACGGTAGTCCTCACAACGCCATCTTCGGCAACGATGATGATCCACAATTGGTGAAGACAAGGTTGAGACAGTGGGCGCAGGTTGTTGCTTGTGCGGTTCGTCAAAGTTCAAGCTGAATGATGATGATGATTGACAATCATGATGATGATGAATGGGTGATAATTGAAGATTAATCGGAGAAAAACTAAACCATGGTTATTAACTTAACCATATTGTGATATAAATGACAAAATTGGAGTACCAAACCGAACCGGCCACGAGGCTTAGGTTAACAAACCCAGATTGGTTCGGTACAAAAAGTAGTTGCTGTTATTATTGATATTATGTTTCTGTCGGTAGCTTTGTTTTTAGTTTTGTACTGTTGTTGTCCAAAATATAACTGTGGTAGATTTTTATATTGTAGTATAGTATAATGTTTTATTTTTTTGTGCAATAATAGTACTAGTAGACGTAGTATAACTATATAAGTTCCTTGAAGTAAGTACGATAGTTTTGTTTTGTGTCAGTAAAAAAAGATGTTTGAAACTTACGC
TARGET=655,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3415
PRIMER_LEFT_PENALTY=0.199246
PRIMER_RIGHT_PENALTY=0.142283
PRIMER_LEFT_SEQUENCE=CACTCCTTCTCCTCACGGTAGT
PRIMER_RIGHT_SEQUENCE=CGGTTTGGTACTCCAATTTTGT
PRIMER_LEFT=524,22
PRIMER_RIGHT=785,22
PRIMER_LEFT_TM=59.801
PRIMER_RIGHT_TM=60.142
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=0.3446
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.336392
PRIMER_LEFT_1_SEQUENCE=CAATTGGTGAAGACAAGGTTGA
PRIMER_RIGHT_1_SEQUENCE=CCATGGTTTAGTTTTTCTCCGA
PRIMER_LEFT_1=582,22
PRIMER_RIGHT_1=732,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.336
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=0.4804
PRIMER_LEFT_2_PENALTY=0.143968
PRIMER_RIGHT_2_PENALTY=0.336392
PRIMER_LEFT_2_SEQUENCE=TAACCCTAAACCTGTCACCACC
PRIMER_RIGHT_2_SEQUENCE=CCATGGTTTAGTTTTTCTCCGA
PRIMER_LEFT_2=458,22
PRIMER_RIGHT_2=732,22
PRIMER_LEFT_2_TM=60.144
PRIMER_RIGHT_2_TM=60.336
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.5001
PRIMER_LEFT_3_PENALTY=0.163658
PRIMER_RIGHT_3_PENALTY=0.336392
PRIMER_LEFT_3_SEQUENCE=GATCCACAATTGGTGAAGACAA
PRIMER_RIGHT_3_SEQUENCE=CCATGGTTTAGTTTTTCTCCGA
PRIMER_LEFT_3=576,22
PRIMER_RIGHT_3=732,22
PRIMER_LEFT_3_TM=59.836
PRIMER_RIGHT_3_TM=60.336
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.5356
PRIMER_LEFT_4_PENALTY=0.199246
PRIMER_RIGHT_4_PENALTY=0.336392
PRIMER_LEFT_4_SEQUENCE=CACTCCTTCTCCTCACGGTAGT
PRIMER_RIGHT_4_SEQUENCE=CCATGGTTTAGTTTTTCTCCGA
PRIMER_LEFT_4=524,22
PRIMER_RIGHT_4=732,22
PRIMER_LEFT_4_TM=59.801
PRIMER_RIGHT_4_TM=60.336
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_03167 Ps_contig_mira-and-tgicl-ass_franssen_28961
SEQUENCE=ATCAACGCAGAGTACGCGGGGACCGCCGAATCCAAAATAATTAAAAAAAAGAAAAAAAAACCAATAGCGTCCCCTAACCCACCGTGCGAGGTCCCTCGACCTCCGCACAGCCAGGGTATTATTATGTCTGACACTCACGCGCCGTCGTCTCCTCCTCCTTCCTTTCTCTTCCACCGTCCACCTTTGTTCAAACAGGGATTTTCTTCTCCGCTTCTGAAGCAGAAATCGTGGTCGCCGGATCTTTATCGCGACGAGGCCTGGCTTCGACGGAAAGGAAACTGGAAGAATCGGAGAAGCAAAAGCGTGACGGATGAAGATGTTGATGAACTCAAAGCCTGCATTGAGTTAGGATTCGGATTCGAATCTTCTCCAGAAGTTGAAACCGATCGGAGACTTGTAGATACTTTACCAGCTCTTGAACTCTATCACGCCGTCAATAAAAGTTACAACGATTCTCGTAACCCTAAACCTGTCACCACCACCGTAACTACTCCATCTTCCTCCGCTGCATCCGATCGTGACGGCACTCCTTCTCCTCACGGTAGTCCTCACAACGCCATCTTCGGCAACGATGATGATCCACAATTGGTGAAGACAAGGTTGAGACAGTGGGCGCAGGTTGTTGCTTGTGCGGTTCGTCAAAGTTCAAGCTGAATGATGATGATGATTGACAATCATGATGATGATGAATGGGTGATAATTGAAGATTAATCGGAGAAAAACTAAACCATGGTTATTAACTTAACCATATTGTGATATAAATGACAAAATTGGAGTACCAAACCGAACCGGCCACGAGGCTTAGGTTAACAAACCCAGATTGGTTCGGTACAAAAAGTAGTTGCTGTTATTATTGATATTATGTTTCTGTCGGTAGCTTTGTTTTTAGTTTTGTACTGTTGTTGTCCAAAATATAACTGTGGTAGATTTTTATATTGTAGTATAGTATAATGTTTTATTTTTTTGTGCAATAATAGTACTAGTAGACGTAGTATAACTATATAAGTTCCTTGAAGTAAGTACGATAGTTTTGTTTTGTGTCAGTAAAAAAAGATGTTTGAAACTTACGC
TARGET=677,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3415
PRIMER_LEFT_PENALTY=0.199246
PRIMER_RIGHT_PENALTY=0.142283
PRIMER_LEFT_SEQUENCE=CACTCCTTCTCCTCACGGTAGT
PRIMER_RIGHT_SEQUENCE=CGGTTTGGTACTCCAATTTTGT
PRIMER_LEFT=524,22
PRIMER_RIGHT=785,22
PRIMER_LEFT_TM=59.801
PRIMER_RIGHT_TM=60.142
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=0.3446
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.336392
PRIMER_LEFT_1_SEQUENCE=CAATTGGTGAAGACAAGGTTGA
PRIMER_RIGHT_1_SEQUENCE=CCATGGTTTAGTTTTTCTCCGA
PRIMER_LEFT_1=582,22
PRIMER_RIGHT_1=732,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.336
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=0.4804
PRIMER_LEFT_2_PENALTY=0.143968
PRIMER_RIGHT_2_PENALTY=0.336392
PRIMER_LEFT_2_SEQUENCE=TAACCCTAAACCTGTCACCACC
PRIMER_RIGHT_2_SEQUENCE=CCATGGTTTAGTTTTTCTCCGA
PRIMER_LEFT_2=458,22
PRIMER_RIGHT_2=732,22
PRIMER_LEFT_2_TM=60.144
PRIMER_RIGHT_2_TM=60.336
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.5001
PRIMER_LEFT_3_PENALTY=0.163658
PRIMER_RIGHT_3_PENALTY=0.336392
PRIMER_LEFT_3_SEQUENCE=GATCCACAATTGGTGAAGACAA
PRIMER_RIGHT_3_SEQUENCE=CCATGGTTTAGTTTTTCTCCGA
PRIMER_LEFT_3=576,22
PRIMER_RIGHT_3=732,22
PRIMER_LEFT_3_TM=59.836
PRIMER_RIGHT_3_TM=60.336
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.5356
PRIMER_LEFT_4_PENALTY=0.199246
PRIMER_RIGHT_4_PENALTY=0.336392
PRIMER_LEFT_4_SEQUENCE=CACTCCTTCTCCTCACGGTAGT
PRIMER_RIGHT_4_SEQUENCE=CCATGGTTTAGTTTTTCTCCGA
PRIMER_LEFT_4=524,22
PRIMER_RIGHT_4=732,22
PRIMER_LEFT_4_TM=59.801
PRIMER_RIGHT_4_TM=60.336
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_03168 Ps_contig_mira-and-tgicl-ass_franssen_28968
SEQUENCE=AATTTTTAAGATCAAACATCAAATAAATATTTCTATATTGTTCAATCTTTACACTCAATTTCAAGTTTATGCAATCAGCTACATCATCATACTGTGTAAAGATAGTATTAAAAATAATCTTAACTGTCTACTAACGATTTAAAAAAATAAACCCAAAAATAACCCGAACCATATTATATGGTCAAGTTCAATCCAAAATTTCAGACATTTAAATATATAATCTTTAATAACCACCTTGGGTACCATATAAACCCAGCTACAAATTCAAAAATAACACTACAGTTCACTTATCAGAGCAATAAAGTTCATCCTATTTCAATACCAGACGCGACGCAACGCAACGCGCTATGGCCGTTACGAAGCCCCTTGAATTGCAGCCGCAGGTTGTTGCATCGCTATATCCTGATTCTGTCTAGCAGCATATCCATGGCCTCCGTATCCGTGACCACCGTAACCCTGCCCGCCGTAATGGTTTCCATTCGAGTCATTATTCCTCCAATGCTTATTTCCCGGACTGCGACCCCAAGAAAGACGCACGGTCTGCTTCCCGATCACTGTTCCATTCAATCCCTGAATAGCTTCCTCAGCATTCTTTCTGTCAGCAAGTTGAACAAAGCCACATCCTTTACCGATTGGAATTTTCACGGAGACAACGTCGCCGTATTGCAAAAACGGTTGTCTGAGATCCTCATCGCTGATTTCAGAATCAAGCCCTCCAACAAATATAGTTGTGTTGTTAGAATCCCCTTCAGATTGGGAGCCTTGGGCCACAGCACCATTAGCAGAGTGACCACCGCCAGCCAACACTACAGCTTGTGAAGAATATTGTTGTGGATTGCCGTAAGTTTTTTGGGCGTTGCAACACCGACTCGCATAGGTCTGCTAGAACAATAAACGCCGTTCATTTCAGTCATTGCCCTTGTCCTTTCATTTTCATCACCGAACCTAACAAAACCATATCCTTTTGAGCGACCAGTATTAGAATCAATGACAACTTTTGCTCCCTTAATAGACGAGAATCTGCTAGCAAACACCTCCTGCAGCATAGCATCGGTGACATCTATGGCTAAGTCTCCCACAAAAACGGAGAGATCAGAAGTAGCCTCAGAAGAACGCCTTTCACCACCACCGCCACCGCCAGCGCTGAACGTAGCCCAATTCAGACGAAACGGCTGATCTGTATTTGGCATCATAGCACCATTAAAGTTCTGCAGGACTTTCTCAGCCGTTCCTCGCGAATAGAACTCGACAAATCCATATCCCTCCGAGACACCAGTTTGCTTATTACGTATGACCTTGGCCGATACAACCTCGCCGGTATGAGCAAAGCAGTTACGAAGAAAAGTCTCGTCCATCCAATGATGAAGGTCACCAAGCCAGATCGTCCTGATCTCTTCAGGAGAAACCTGTTTCTGCTGATGCTGCTGCTGGTGATGAAGATGATGACCATGGCCATGACCATGACCATGATGACTCTGAGTACTCGGCGGCGGCGACGGTTGCTGCTGGTGAGGCGGCGCCTGATAATGATGAGGATGAACATACGACATGTAATGCTGTGGATACATCATCATCTGCTGCTGCATCATAGCCATAGCAGTAGCAGGATACTGCATGGACATCCATTGCTGCTGATACTGATGCGGCTGCGCCCACTGCTGCTGCTGAACGTTCTGGCTCTGGTTCTGATTCGGATTCTGGTTCGGGTTCTGGCTCTGGTTCTGGTTCTGGCTTGGGTTCTGGCTCGGATTCAGATCTCCGGAGCTGGTTTGTTGAGACATAGTTCAAAATTTGTTTCTTTCTCTGTTTTTTCTCTTTCTCGGACTTGGTCTGGTCGAGTTGGTCTTAACAGAGAAAAACCGAGAAAGAGACAGAGAAAGAAGAAAGAGAAGGCGGAAAGACGAGTAGGGTTGGGTTTTAAAGGAACAACAACAGCAACAGCAATCAACAAAAACAACAACACGCACACTGGTTCTATTGTATTGTACTATNCTTTTTTTTATTGCAAATTAA
TARGET=1656,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2149
PRIMER_LEFT_PENALTY=0.161579
PRIMER_RIGHT_PENALTY=0.053348
PRIMER_LEFT_SEQUENCE=GTAGCAGGATACTGCATGGACA
PRIMER_RIGHT_SEQUENCE=GAACCAGAATCCGAATCAGAAC
PRIMER_LEFT=1599,22
PRIMER_RIGHT=1703,22
PRIMER_LEFT_TM=60.162
PRIMER_RIGHT_TM=59.947
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=105
PRIMER_PAIR_PENALTY_1=0.2961
PRIMER_LEFT_1_PENALTY=0.161579
PRIMER_RIGHT_1_PENALTY=0.134571
PRIMER_LEFT_1_SEQUENCE=ACATCCATTGCTGCTGATACTG
PRIMER_RIGHT_1_SEQUENCE=CCAAGTCCGAGAAAGAGAAAAA
PRIMER_LEFT_1=1618,22
PRIMER_RIGHT_1=1828,22
PRIMER_LEFT_1_TM=60.162
PRIMER_RIGHT_1_TM=59.865
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.2961
PRIMER_LEFT_2_PENALTY=0.161579
PRIMER_RIGHT_2_PENALTY=0.134571
PRIMER_LEFT_2_SEQUENCE=GTAGCAGGATACTGCATGGACA
PRIMER_RIGHT_2_SEQUENCE=CCAAGTCCGAGAAAGAGAAAAA
PRIMER_LEFT_2=1599,22
PRIMER_RIGHT_2=1828,22
PRIMER_LEFT_2_TM=60.162
PRIMER_RIGHT_2_TM=59.865
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.2976
PRIMER_LEFT_3_PENALTY=0.161579
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=ACATCCATTGCTGCTGATACTG
PRIMER_RIGHT_3_SEQUENCE=GACCAAGTCCGAGAAAGAGAAA
PRIMER_LEFT_3=1618,22
PRIMER_RIGHT_3=1830,22
PRIMER_LEFT_3_TM=60.162
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.2976
PRIMER_LEFT_4_PENALTY=0.161579
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=GTAGCAGGATACTGCATGGACA
PRIMER_RIGHT_4_SEQUENCE=GACCAAGTCCGAGAAAGAGAAA
PRIMER_LEFT_4=1599,22
PRIMER_RIGHT_4=1830,22
PRIMER_LEFT_4_TM=60.162
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_03169 Ps_contig_mira-and-tgicl-ass_franssen_28972
SEQUENCE=ATAATATGAGAGAAGAACCTGTTATTTACATCAATGGGAAACCATTTGTTCTCCGTGAAGTTGAAGACCGTACAAAAACATGCGGGAATATACGGGAATTGGTCGTGAAAGGGTGGAGAAAATGGAAGCCCGGTTAAAAGAAGATATCCTAAGGGAAGCTGAGCAGTATAACAATGCCATATCATTGATGTTGTGAAGACTGGATCTGAACACAATCCTGCAGGTGGAGGAGGTGCGGTTAGCAAATTCTGATTAAAGGATCCATGACCAGAAAAATAATAAAAAATTGAAGAAAAAATACTTAACCTAACTTCATTCANATAATATTGTAAATGCAAGAAGGGTGTTTAGTTTCTTGTATAAGTGTTTAGTTTTTATTATTATTATTATTATTATGTATTATTTAATTAATNTAAGGTGTGTTTATTGTGTATG
TARGET=375,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03170 Ps_contig_mira-and-tgicl-ass_franssen_28973
SEQUENCE=AACTTAAATTTACATTTTCTATTAATAAATTGGAACATTGATCACATGAAATTATTTAGAACATTGACGTAGTATAGGACATTAATTATTAGAGTTATAATATATGGCCAAAGCTTAGGAATATATTGTTATATAATTGCTTTCATAGCCATTGCCAATTACACCAAATTAAAACCACACCTTTACATTAATTAATAATCATAATAATAATAATAATAATAAAAACTAAACACTTATACAAGAAACTAAACACCCTTCTTGCATTTTACAATATTATATGAAATTGAAAGTTTAGGTTAAGTATTTTTTTCTTCAATTTTTTTATTATTTTTCTGGTCATGGATCCTTTAATCAGAATTTGCTAACCGCACCTCCTCCACCTGCAGGATTGTGTTCAGATCCAGTCTTCACAACATCAATGATATGGCCACCACTCAGTTCTTGACCCTTCAATGGTGCCTGAAGCACTGGTGTTGCATTTCTATATATCAAATATATCACCATCTGAATTATGCCAAACACAAACCCAAGTGTATTTGGTAGAGCAACATAGTAATCCTTGAGGAGAAGGCCATAGAAGAACCACATAACAGCATTGATGGTTAAGAAGAAGGACAAGAAAAATGGCATGTATTCGACACTCCTCGTTCTTATAACTTTGCTGATGACGAAGAGAGGTGCAGCGAAAACACTTATGTTGAAAACAAGACAAATCCATCCGATAATAGAAAGACGTTTCGCTCCCTTGGCGAGGTAAAGAGTTGATAGAAGCATGGCTCCGAATCCGAACACATTCAGCAAGAGAAGTAGTTTTATGGTAGAAAGCCTAGATTTCTTTGAGGCATAAATCAGGAAGATTATGAGATAAGCTGATTCAACAATAATTCCAAAAGTGTTAATGGTGATGAGAAGAAGAGCAGCTTCTCTTTTCACAAATGCATAGTAAATCCAAAGCATTGCACTGAAAAGTGCAACAACATAAGGAAGTGACTGAAATCCTTCAGCAGATTTTTTCTTGAAGATTATGTAAAAAGTTGGCACAGGTGAAAGAAACACTGCAAAGGAAATGATGTTGCCTAGAAGGCCAAAGACAAAAGCCAAAGTTTCACGATGCATGGCCATTTCTTGAATCTAAGTAAAAAACTGTTGAAAACTTTGGAATAATTTAAGAATAAGTGTCACTCTCTTTCTTTCTCAAAGTGATCAAAACCCTTAGTTGAAGGTATCACTTTGTTTCTCTCTCTAAATTCCACCCCCCGACGTACTCTGCGTTGAATAACCCACTGCTT
TARGET=201,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5311
PRIMER_LEFT_PENALTY=0.140609
PRIMER_RIGHT_PENALTY=0.390521
PRIMER_LEFT_SEQUENCE=TCATAGCCATTGCCAATTACAC
PRIMER_RIGHT_SEQUENCE=AGAACTGAGTGGTGGCCATATC
PRIMER_LEFT=142,22
PRIMER_RIGHT=441,22
PRIMER_LEFT_TM=59.859
PRIMER_RIGHT_TM=60.391
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=5.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.6381
PRIMER_LEFT_1_PENALTY=0.140609
PRIMER_RIGHT_1_PENALTY=0.497481
PRIMER_LEFT_1_SEQUENCE=TCATAGCCATTGCCAATTACAC
PRIMER_RIGHT_1_SEQUENCE=GTGCGGTTAGCAAATTCTGATT
PRIMER_LEFT_1=142,22
PRIMER_RIGHT_1=370,22
PRIMER_LEFT_1_TM=59.859
PRIMER_RIGHT_1_TM=60.497
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=229
PRIMER_PAIR_PENALTY_2=0.7798
PRIMER_LEFT_2_PENALTY=0.140609
PRIMER_RIGHT_2_PENALTY=0.639172
PRIMER_LEFT_2_SEQUENCE=TCATAGCCATTGCCAATTACAC
PRIMER_RIGHT_2_SEQUENCE=AACTGAGTGGTGGCCATATCAT
PRIMER_LEFT_2=142,22
PRIMER_RIGHT_2=439,22
PRIMER_LEFT_2_TM=59.859
PRIMER_RIGHT_2_TM=60.639
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=1.2372
PRIMER_LEFT_3_PENALTY=0.140609
PRIMER_RIGHT_3_PENALTY=1.096569
PRIMER_LEFT_3_SEQUENCE=TCATAGCCATTGCCAATTACAC
PRIMER_RIGHT_3_SEQUENCE=TGATTAAAGGATCCATGACCAG
PRIMER_LEFT_3=142,22
PRIMER_RIGHT_3=353,22
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=58.903
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=1.3042
PRIMER_LEFT_4_PENALTY=0.140609
PRIMER_RIGHT_4_PENALTY=1.163603
PRIMER_LEFT_4_SEQUENCE=TCATAGCCATTGCCAATTACAC
PRIMER_RIGHT_4_SEQUENCE=TGATGTTGTGAAGACTGGATCTG
PRIMER_LEFT_4=142,22
PRIMER_RIGHT_4=417,23
PRIMER_LEFT_4_TM=59.859
PRIMER_RIGHT_4_TM=60.164
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_03171 Ps_contig_mira-and-tgicl-ass_franssen_28980
SEQUENCE=GGGGGACAAACGCGAGACATCTTCATTCTCTCTCTCTCCTTTCTCTCTCTGAAGATGGCTTCACGCAGACTCGTATCGTCTCTGATTCGATCTTCTCTTCGTCGATCCTCATCGAAACCCTCAATTTCCGCCTCAACTTCGAGGCTCACTTCTCAATCCCGTGCCTCTCCTTGCGGCTATCTTCTTAACCGCGTAGCCGATTACGCTACTTCGGCCGCAGCTGCTGCTGCTCCTCCATCTCCGCCTCCAGCGAAAAAGGAGGTTCCCGGAGGTGGTAAAATCACCGATGAGTTCACCGGTAAGGGTGCGATCGGGCATGTTTGTCAGGTTATTGGTGCCGTCGTCGATGTCAGATTCGAAGAGGGTTTGCCTCCGATCTTGACTGCTCTTGAGCTTTTGGATCATGAAACGCGGTTGGTGTTGGAAGTTGCGCAGCATTTGGGCGAGGGCGTTGTCAGAACTATTGCTATGGATGCTACTGAAGGTGTTGTTAGAGGGTGGCGTGTGCTCAACACTGGATCCCCTATCAGTGTTCCTGTCGGTAGGGCAACCCTGGGCCGTATCATGAATGTTATTGGAGAGCCTATTGACGAGAAGGGTGAATTGAAAACTGAGCATTTTTTGCCCATCCATAGAGAAGCCCCTTCTTTTGTTGAGCAAGCAACTGAACAGGAAATTCTTGTTACTGGTATCAAGGTTGTTGACCTGCTTGCACCATACCAAAGAGGAGGAAAGATTGGGTTGTTTGGTGGTGCTGGTGTTGGAAAGACTGTGCTTATTATGGAACTTATCAACAATGTTGCTAAGGCGCATGGTGGTTTCTCTGTGTTTGCTGGTGTTGGAGAACGAACTCGAGAGGGTAATGACCTGTACAGAGAAATGATTGAGAGTGGTGTCATTAAGCTGGGTGATAAGCAGAGTGAAAGCAAATGTGCTCTTGTGTATGGTCAAATGAACGAACCCCCTGGTGCCCGTGCCCGTGTTGGTCTTACTGGACTTACTGTTGCTGAGCACTTCCGTGATGCTGAAGGGCAAGATGTGCTTCTTTTTGTTGACAACATTTTCCGTTTTACTCAAGCTAACTCAGAGGTGTCTGCTTTACTTGGTCGTATCCCATCTGCTGTTGGTTACCAACCAACATTGTCTACTGATCTTGGAGGTCTTCAAGAGCGTATTACAACCACCAAAAAGGGTTCAATTACCTCTGTCCAAGCTATCTACGTGCCTGCTGACGATTTGACAGATCCTGCCCCCGCCACTACCTTTGCTCACTTGGATGCCACAACAGTGTTGTCACGATCGATCTCTGAGCTTGGCATCTATCCTGCTGTCGACCCATTGGATTCTACATCTCGTATGCTTTCCCCACTTATTTTGGGTGAGGCCCACTATGAAACCGCTCGTGGTGTACAGAAAGTACTTCAAAACTACAAGAATCTTCAAGATATCATTGCAATTTTGGGAATGGATGAGCTCAGTGAAGACGATAAATTGACTGTTGCCCGTGCTCGTAAGATTCAGCGATTCTTGAGCCAGCCTTTCCATGTTGCAGAAGTCTTCACTGGTGCCCCTGGCAAATATGTTGACTTGAAGGAGAACATTACTAGTTTCCAGGGTGTGTTGGATGGCAAGTACGATGACCTTTCAGAGCAGTCATTTTACATGGTTGGTGGTATTGAAGAGGTCATTGCAAAGGCAGAGAAGATTGCCAAGGAATCAGCTGCATCTTCATCTTAATCTCACAAATCCCTCCCATCATTTTGTCTTTTCTTTATAATTGCGAAAATTTAATAATTTAGGAACTCTTTCTTGGCAATGCCAAACAACAAAAATTTTCCATTTTATAATTTTTATAATTTGAATGGTCATTCCTTTCATTTTGAGTGTTGTTGCTGACGGTGAGATCATCCAAATTATGTGGCCGCTTTTTTTCCGTTGTGTTATTTAATAAGTGGATGATGCAACAGATTGTACATTTTTATTTGTTAAGAACTTGGATGCATCCATTGTTAAAGTTTAGAGCTGGTTCATGTGTTGTGAAGCATACTTTTACGTCATTGATGAATTTTCAAACAATTCAACAATATTGTCAATATATTTTATTCTGCAGCTAATTAATATATTAGACGGGCTGGTGCCAAGTGACAACTTGTTATCACCACTGATAAAATTTTAATTTGATATACCG
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0474
PRIMER_LEFT_PENALTY=1.043808
PRIMER_RIGHT_PENALTY=0.003602
PRIMER_LEFT_SEQUENCE=ACAAACGCGAGACATCTTCATT
PRIMER_RIGHT_SEQUENCE=TTAAGAAGATAGCCGCAAGGAG
PRIMER_LEFT=5,22
PRIMER_RIGHT=187,22
PRIMER_LEFT_TM=61.044
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=1.1596
PRIMER_LEFT_1_PENALTY=1.043808
PRIMER_RIGHT_1_PENALTY=0.115779
PRIMER_LEFT_1_SEQUENCE=ACAAACGCGAGACATCTTCATT
PRIMER_RIGHT_1_SEQUENCE=GTTAAGAAGATAGCCGCAAGGA
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=188,22
PRIMER_LEFT_1_TM=61.044
PRIMER_RIGHT_1_TM=59.884
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=1.2487
PRIMER_LEFT_2_PENALTY=1.245123
PRIMER_RIGHT_2_PENALTY=0.003602
PRIMER_LEFT_2_SEQUENCE=ACAAACGCGAGACATCTTCAT
PRIMER_RIGHT_2_SEQUENCE=TTAAGAAGATAGCCGCAAGGAG
PRIMER_LEFT_2=5,21
PRIMER_RIGHT_2=187,22
PRIMER_LEFT_2_TM=59.755
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=1.2672
PRIMER_LEFT_3_PENALTY=1.263605
PRIMER_RIGHT_3_PENALTY=0.003602
PRIMER_LEFT_3_SEQUENCE=CAAACGCGAGACATCTTCATT
PRIMER_RIGHT_3_SEQUENCE=TTAAGAAGATAGCCGCAAGGAG
PRIMER_LEFT_3=6,21
PRIMER_RIGHT_3=187,22
PRIMER_LEFT_3_TM=60.264
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=1.2854
PRIMER_LEFT_4_PENALTY=1.043808
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=ACAAACGCGAGACATCTTCATT
PRIMER_RIGHT_4_SEQUENCE=CTCATCGGTGATTTTACCACCT
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=290,22
PRIMER_LEFT_4_TM=61.044
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_03172 Ps_contig_mira-and-tgicl-ass_franssen_28980
SEQUENCE=GGGGGACAAACGCGAGACATCTTCATTCTCTCTCTCTCCTTTCTCTCTCTGAAGATGGCTTCACGCAGACTCGTATCGTCTCTGATTCGATCTTCTCTTCGTCGATCCTCATCGAAACCCTCAATTTCCGCCTCAACTTCGAGGCTCACTTCTCAATCCCGTGCCTCTCCTTGCGGCTATCTTCTTAACCGCGTAGCCGATTACGCTACTTCGGCCGCAGCTGCTGCTGCTCCTCCATCTCCGCCTCCAGCGAAAAAGGAGGTTCCCGGAGGTGGTAAAATCACCGATGAGTTCACCGGTAAGGGTGCGATCGGGCATGTTTGTCAGGTTATTGGTGCCGTCGTCGATGTCAGATTCGAAGAGGGTTTGCCTCCGATCTTGACTGCTCTTGAGCTTTTGGATCATGAAACGCGGTTGGTGTTGGAAGTTGCGCAGCATTTGGGCGAGGGCGTTGTCAGAACTATTGCTATGGATGCTACTGAAGGTGTTGTTAGAGGGTGGCGTGTGCTCAACACTGGATCCCCTATCAGTGTTCCTGTCGGTAGGGCAACCCTGGGCCGTATCATGAATGTTATTGGAGAGCCTATTGACGAGAAGGGTGAATTGAAAACTGAGCATTTTTTGCCCATCCATAGAGAAGCCCCTTCTTTTGTTGAGCAAGCAACTGAACAGGAAATTCTTGTTACTGGTATCAAGGTTGTTGACCTGCTTGCACCATACCAAAGAGGAGGAAAGATTGGGTTGTTTGGTGGTGCTGGTGTTGGAAAGACTGTGCTTATTATGGAACTTATCAACAATGTTGCTAAGGCGCATGGTGGTTTCTCTGTGTTTGCTGGTGTTGGAGAACGAACTCGAGAGGGTAATGACCTGTACAGAGAAATGATTGAGAGTGGTGTCATTAAGCTGGGTGATAAGCAGAGTGAAAGCAAATGTGCTCTTGTGTATGGTCAAATGAACGAACCCCCTGGTGCCCGTGCCCGTGTTGGTCTTACTGGACTTACTGTTGCTGAGCACTTCCGTGATGCTGAAGGGCAAGATGTGCTTCTTTTTGTTGACAACATTTTCCGTTTTACTCAAGCTAACTCAGAGGTGTCTGCTTTACTTGGTCGTATCCCATCTGCTGTTGGTTACCAACCAACATTGTCTACTGATCTTGGAGGTCTTCAAGAGCGTATTACAACCACCAAAAAGGGTTCAATTACCTCTGTCCAAGCTATCTACGTGCCTGCTGACGATTTGACAGATCCTGCCCCCGCCACTACCTTTGCTCACTTGGATGCCACAACAGTGTTGTCACGATCGATCTCTGAGCTTGGCATCTATCCTGCTGTCGACCCATTGGATTCTACATCTCGTATGCTTTCCCCACTTATTTTGGGTGAGGCCCACTATGAAACCGCTCGTGGTGTACAGAAAGTACTTCAAAACTACAAGAATCTTCAAGATATCATTGCAATTTTGGGAATGGATGAGCTCAGTGAAGACGATAAATTGACTGTTGCCCGTGCTCGTAAGATTCAGCGATTCTTGAGCCAGCCTTTCCATGTTGCAGAAGTCTTCACTGGTGCCCCTGGCAAATATGTTGACTTGAAGGAGAACATTACTAGTTTCCAGGGTGTGTTGGATGGCAAGTACGATGACCTTTCAGAGCAGTCATTTTACATGGTTGGTGGTATTGAAGAGGTCATTGCAAAGGCAGAGAAGATTGCCAAGGAATCAGCTGCATCTTCATCTTAATCTCACAAATCCCTCCCATCATTTTGTCTTTTCTTTATAATTGCGAAAATTTAATAATTTAGGAACTCTTTCTTGGCAATGCCAAACAACAAAAATTTTCCATTTTATAATTTTTATAATTTGAATGGTCATTCCTTTCATTTTGAGTGTTGTTGCTGACGGTGAGATCATCCAAATTATGTGGCCGCTTTTTTTCCGTTGTGTTATTTAATAAGTGGATGATGCAACAGATTGTACATTTTTATTTGTTAAGAACTTGGATGCATCCATTGTTAAAGTTTAGAGCTGGTTCATGTGTTGTGAAGCATACTTTTACGTCATTGATGAATTTTCAAACAATTCAACAATATTGTCAATATATTTTATTCTGCAGCTAATTAATATATTAGACGGGCTGGTGCCAAGTGACAACTTGTTATCACCACTGATAAAATTTTAATTTGATATACCG
TARGET=220,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1413
PRIMER_LEFT_PENALTY=0.115779
PRIMER_RIGHT_PENALTY=0.025566
PRIMER_LEFT_SEQUENCE=TCCTTGCGGCTATCTTCTTAAC
PRIMER_RIGHT_SEQUENCE=ATCCAAAAGCTCAAGAGCAGTC
PRIMER_LEFT=167,22
PRIMER_RIGHT=401,22
PRIMER_LEFT_TM=59.884
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=0.2138
PRIMER_LEFT_1_PENALTY=0.188254
PRIMER_RIGHT_1_PENALTY=0.025566
PRIMER_LEFT_1_SEQUENCE=CTCAACTTCGAGGCTCACTTCT
PRIMER_RIGHT_1_SEQUENCE=ATCCAAAAGCTCAAGAGCAGTC
PRIMER_LEFT_1=131,22
PRIMER_RIGHT_1=401,22
PRIMER_LEFT_1_TM=60.188
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=0.3573
PRIMER_LEFT_2_PENALTY=0.115779
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=TCCTTGCGGCTATCTTCTTAAC
PRIMER_RIGHT_2_SEQUENCE=CTCATCGGTGATTTTACCACCT
PRIMER_LEFT_2=167,22
PRIMER_RIGHT_2=290,22
PRIMER_LEFT_2_TM=59.884
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=0.4298
PRIMER_LEFT_3_PENALTY=0.188254
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=CTCAACTTCGAGGCTCACTTCT
PRIMER_RIGHT_3_SEQUENCE=CTCATCGGTGATTTTACCACCT
PRIMER_LEFT_3=131,22
PRIMER_RIGHT_3=290,22
PRIMER_LEFT_3_TM=60.188
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=0.4393
PRIMER_LEFT_4_PENALTY=0.413759
PRIMER_RIGHT_4_PENALTY=0.025566
PRIMER_LEFT_4_SEQUENCE=GCTATCTTCTTAACCGCGTAGC
PRIMER_RIGHT_4_SEQUENCE=ATCCAAAAGCTCAAGAGCAGTC
PRIMER_LEFT_4=175,22
PRIMER_RIGHT_4=401,22
PRIMER_LEFT_4_TM=59.586
PRIMER_RIGHT_4_TM=60.026
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_03173 Ps_contig_mira-and-tgicl-ass_franssen_28983
SEQUENCE=GACAAGACAGNTTTTCATATAAAATCATTCATATATCATCATCACCATACTGTAAAAGCTTAAAGGTAAAACGGTATACAGGTTAATATCTTGACTACATACAAAATCATGCCATGCACCTGGTATCATTCCTCGTCATATCATATATATAATCACAAGAAAATCGAAATAACAAGATAAATGTCTAGGAGAGTGGCATTTATTTATTCCGAAGAACGATTGGGAGGACAATTACGAGAATCAAGGCTATCAAAATTGCCAATACAATCAGCTTTATCTTCATATTCTGCAGCCACATTTTTCTGCGGATTTTGGTCCCTGAGTTCCTGAAGTCTTGTGCCTGGTGATGAAGGTTCTCAGTCTTGTCAACCAAAAGCTCTATCTTTTCCCCCCTGTCCAGAACCTTTTCAATATTCTCCATCATGACACCTTTGACTTCAGAAACCTGAGCTTTCACCTTAGCAAGCTTGCTCACCTCCTCAGGATGATCAACACAGTACTGCATATGTTCCTTCAATTTAGGCCCAAATTCCTTATTAAGACTGTTAGGTGCAGCAGTAGAAGCCTTCCCACCGCCATATTTCGCAACAAAATCATCCTTCACACGCTCCAGAAAAGCCACAGGCACTTGTCTTCCAACCGATTCATCCGCAACAACACAATACGTGTAGCCATTATCGACGAGGTAATTGAAGGTGTGGCCATCACAGTTGTAGGTGAATTTGTTGTTAGAAGCTGGGAGCTTCTGAAGACACTGAAACGCGATGGAATTGAAATTTCCGCTGAATTCGGTGTACTCGGAGAGAAGAACACTTCCTCGAGAAACGAACGCGTAGATCAGAGATCTTTGATTTTGTCCCATCTTCTTCTCCACAGATCTATCGAACAACGGTTTCTTGTGATTTTGGAGTTTGCTATATTCTCTCCAGCCTTTTCTTTCTCTTTTCTTTTCTAGAGAGAGAGGGTTTATAGAGAGAGAAAGAGTCCCCGACGTACTCTGCGTTGATACCCACTGCTT
TARGET=954,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2219
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.189596
PRIMER_LEFT_SEQUENCE=TATCGAACAACGGTTTCTTGTG
PRIMER_RIGHT_SEQUENCE=CAGTGGGTATCAACGCAGAGTA
PRIMER_LEFT=879,22
PRIMER_RIGHT=1014,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=60.190
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=0.2635
PRIMER_LEFT_1_PENALTY=0.073866
PRIMER_RIGHT_1_PENALTY=0.189596
PRIMER_LEFT_1_SEQUENCE=TCCCATCTTCTTCTCCACAGAT
PRIMER_RIGHT_1_SEQUENCE=CAGTGGGTATCAACGCAGAGTA
PRIMER_LEFT_1=856,22
PRIMER_RIGHT_1=1014,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.190
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.3200
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.189596
PRIMER_LEFT_2_SEQUENCE=AATTCGGTGTACTCGGAGAGAA
PRIMER_RIGHT_2_SEQUENCE=CAGTGGGTATCAACGCAGAGTA
PRIMER_LEFT_2=785,22
PRIMER_RIGHT_2=1014,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=60.190
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.3219
PRIMER_LEFT_3_PENALTY=0.132262
PRIMER_RIGHT_3_PENALTY=0.189596
PRIMER_LEFT_3_SEQUENCE=TCCACAGATCTATCGAACAACG
PRIMER_RIGHT_3_SEQUENCE=CAGTGGGTATCAACGCAGAGTA
PRIMER_LEFT_3=869,22
PRIMER_RIGHT_3=1014,22
PRIMER_LEFT_3_TM=60.132
PRIMER_RIGHT_3_TM=60.190
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.3948
PRIMER_LEFT_4_PENALTY=0.205226
PRIMER_RIGHT_4_PENALTY=0.189596
PRIMER_LEFT_4_SEQUENCE=AACACTTCCTCGAGAAACGAAC
PRIMER_RIGHT_4_SEQUENCE=CAGTGGGTATCAACGCAGAGTA
PRIMER_LEFT_4=808,22
PRIMER_RIGHT_4=1014,22
PRIMER_LEFT_4_TM=59.795
PRIMER_RIGHT_4_TM=60.190
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_03174 Ps_contig_mira-and-tgicl-ass_franssen_28998
SEQUENCE=ATTNNTTTTCATGATCGACACACACAATACAAAACACTACTCCACCCAAACAGATAACCAAATCCTCTTCTCCAACACTCTCGATTCTCACTCAATTTCTCTCCTTCAATTCAACCATGGCACTTTCTTCCACCGCCGCAACCACTTCTTCCAAACTCAAACTATCTAACCCACCGTCACTTTCCCACACTTTCACCGCCTCCGCCTCCGCCTCCGTTTCAAACTCAACATCGTTCCGTCCCAAACTAACCCTAACTCGCCTCTCTTCCTCCTTCCTCAACCCTTCCACAATCCTCCACCTCACTCCCTCTCAACGCACCAACCGTCCTTCTTCCTCTCCCTTCACCGTCCGCGCCGCCCGTGGCAAATTCGAGCGCAAGAAACCTCATCTCAACATCGGTACAATTGGCCACGTCGACCATGGCAAAACCACTCTCACTGCTGCTCTCACTATGGCTCTCGCTTCCCTCGGTAACAGCGCCCCTAAAAAGTACGACGAAATTGACGCTGCTCCCGAAGAGCGTGCACGTGGAATTACAATTAACACTGCAACCGTTGAGTACGAGACTGAAACTCGTCACTACGCTCATGTCGATTGTCCCGGTCACGCTGATTACGTCAAGAATATGATCACTGGTGCCGCTCAAATGGACGGCGCTATCCTCGTTGTCTCCGGCGCCGATGGTCCCATGCCTCAAACTAAAGAACACATCCTTCTCGCCAAGCAGGTTGGTGTTCCGAGCGTGGTTGTGTTTTTGAACAAGCAGGACCAAGTGGATGATGAGGAGCTTCTTGAGCTTGTGGAGCTTGAAGTTCGGGAGCTTCTCTCATCTTATGAGTTTCCTGGCGATGACATTCCGATTGTTTCTGGTTCGGCGCTCTTGGCCTTGGAAGCATTAATGGCAAACCCTACTCTTAAACGTGGGGATAACCAGTGGGTGGATAAGATTTATCAGCTTATGGATGAAGTTGATAAATATATCCCAATCCCTCAACGTCAGACGGAACTCCCCTTTTTGCTTGCCATTGAGGATGTGTTTTCAATCACTGGTCGTGGAACAGTCGCCACTGGGAGAATTGAAAGAGGGCTGGTTAAAGTTGGTGATGTGGTTGACCTTGTCGGTTTGAGGGAAACAAGGAACACTACCGTGACGGGTGTAGAAATGTTCCAAAAGATTTTGGATGATGCTATGGCGGGTGACAATGTGGGGTTGTTGTTGAGAGGTATTCAGAAGATTGACATTCAAAGAGGGATGGTTTTGGCTAAGCCCGGAACTATCACTCCTCACTCTAAGTTTAGTGCTATTGTATATGTTTTGAAGAAGGAAGAAGGTGGAAGACACTCACCTTTCTTTGCTGGTTATAGGCCTCAATTTTACATGAGGACGACAGATGTAACTGGAAAAGTTACTTCTATTATGAATGATAAGGATGAGGAGTCGAAGATGGTGATGCCTGGTGATCGTGTGAAGATCGTGGTGGAACTCATTGTTCCCGTGGCTATCGAACAAGGAATGAGGTTCGCTATTAGAGAAGGAGGGAAGACCGTTGGAGCTGGTGTTATTGGAGCTATCATTGAGTGAATGAAGATTTCTATCCAACGATGCAGCACCACTCTTTAAACGGGGTTCTTTATTTATTTATTTTTAATGCAATCCATTAATTGACTTTTGCCCTTTTATACAACTTGTCTGTGGTAATTTATAGGGTTAAATTTTAACTTCGGGATGATCTTTAGTCAAATTGGGTACTATTTTTCCATCAGAATGTTTTGTTGTTGTTACTTGCTTATACCTGTTTGTTCTGTTACCGTTGAATTCCTTAAATTGCATCAACGATGAGATTTTGGTTGAGGAATCAACCGTTACAGTTTTCCTCTTTATTAGATAGAGCTGAACGATGTTACTTATCATATTCNGTGGTTGAAACAATAACTTAATTGGTGCCCTTTTCATATCCATGTTAAAATGGATAGCATAAATTATATGAAAATCTAAGCAGAATTAAAGTTT
TARGET=1632,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5142
PRIMER_LEFT_PENALTY=0.088700
PRIMER_RIGHT_PENALTY=0.425548
PRIMER_LEFT_SEQUENCE=ATTAGAGAAGGAGGGAAGACCG
PRIMER_RIGHT_SEQUENCE=CAACGGTAACAGAACAAACAGG
PRIMER_LEFT=1526,22
PRIMER_RIGHT=1814,22
PRIMER_LEFT_TM=60.089
PRIMER_RIGHT_TM=59.574
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.5790
PRIMER_LEFT_1_PENALTY=0.266650
PRIMER_RIGHT_1_PENALTY=0.312329
PRIMER_LEFT_1_SEQUENCE=ACCACTCTTTAAACGGGGTTCT
PRIMER_RIGHT_1_SEQUENCE=GGTTGATTCCTCAACCAAAATC
PRIMER_LEFT_1=1610,22
PRIMER_RIGHT_1=1862,22
PRIMER_LEFT_1_TM=60.267
PRIMER_RIGHT_1_TM=59.688
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.6190
PRIMER_LEFT_2_PENALTY=0.266650
PRIMER_RIGHT_2_PENALTY=0.352395
PRIMER_LEFT_2_SEQUENCE=ACCACTCTTTAAACGGGGTTCT
PRIMER_RIGHT_2_SEQUENCE=CCTCAACCAAAATCTCATCGTT
PRIMER_LEFT_2=1610,22
PRIMER_RIGHT_2=1854,22
PRIMER_LEFT_2_TM=60.267
PRIMER_RIGHT_2_TM=60.352
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.6922
PRIMER_LEFT_3_PENALTY=0.266650
PRIMER_RIGHT_3_PENALTY=0.425548
PRIMER_LEFT_3_SEQUENCE=ACCACTCTTTAAACGGGGTTCT
PRIMER_RIGHT_3_SEQUENCE=CAACGGTAACAGAACAAACAGG
PRIMER_LEFT_3=1610,22
PRIMER_RIGHT_3=1814,22
PRIMER_LEFT_3_TM=60.267
PRIMER_RIGHT_3_TM=59.574
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=0.9453
PRIMER_LEFT_4_PENALTY=0.519744
PRIMER_RIGHT_4_PENALTY=0.425548
PRIMER_LEFT_4_SEQUENCE=TCGCTATTAGAGAAGGAGGGAA
PRIMER_RIGHT_4_SEQUENCE=CAACGGTAACAGAACAAACAGG
PRIMER_LEFT_4=1521,22
PRIMER_RIGHT_4=1814,22
PRIMER_LEFT_4_TM=59.480
PRIMER_RIGHT_4_TM=59.574
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_03175 Ps_contig_mira-and-tgicl-ass_franssen_29000
SEQUENCE=TAGTCGGGGACCTCAAATTGAAACAACCTTACCTTAAACCTTCTCAACCCAACAACAACAACAAAAACAACAACATGGGCAGTGTTGAAATTCCAACAAAGGTTCTTACCAACACATCTGCTCAAATTAAGATGCCTGTGGTTGGAATGGGATCAGCACCTGACTTCACATGCAAGAAAGACACTAAAGAAGCAATCATCGAAGCCATCAAACAAGGTTACAGACACTTTGATACTGCTGCTGCTTATGGCTCCGAACAAGCTCTTGGTGAAGCTTTGAATGAGGCTATTCAACTTGGTCTTGTCACTAGAGAACAGCTTTTTGTTACTTCTAAACTCTGGGTTACTGAAAATCATCCTCACCTTGTTCTTCCTGCTCTACAAAAATCTCTCAAGACTCTTCAGTTGGATTACTTGGATTTGTATTTGATTCATTGGCCACTTAGTTCTCAGCCCGGAAAGTTTTCATTTCCAATTGATGTGGCTGATCTACTACCATTTGATGTAAAAGGTGTGTGGGAATCCATGGAAGAAGCTTTGAGACTTGGACTCACGAAAGCTATTGGTGTCAGTAACTTCTCTGTCAAGAAACTTCAAAAGCTACTATCTGTTGCCACTGTTCTTCCTGCTGTTAATCAAGTGGAGATGAACCTTGCATGGCAACAAAAGAAGCTGAGAGAGTTTTGCAATGAAAATGGAATAGTGTTAACTGCATATTCACCGTTGAGGAAAGGTGCAAGTAGAGGAGCCAATGAGGTTATGGAGAATGATATGCTTAAACAGATTGCAGATGCTCATGGAAAGTCCGTTGCACAAATTTCTCTGAGATGGTTATATGAACAAGGAATCACTTTTGTTCCAAAGAGCTATGATAAGGAGAGAATGAGTCAAAATTTGAGTATCTTTGATTGGACACTGACAAAGGAGGATCATGAGAAAATTGATCAAATTAAGCAGAACCGTTTGATCCCTGGACCAACCAAGCCGAGTCTCAATGATCTTTGGGATGATGAAATATAAAAGAAAAGTTGCTAAAGTTATTCAAATTTGCTTTGGCGTCTATCTTTTGTATTTAAGTATTTTATACTACTTGTTTCTTTTGAGCTTTGAATTACCAATGTTTTTGTATTGGGTTTAAATAATCAATTTTATATTTTTATAATAGTGTTTTATTTTACTGTCTTGAAAATTGTAATTTTTATACTAAGAAACAAGTAGCCCAACAAGCAAAATTATACTAAGAAGTTGAAGGAGTGGTATACAACTAAAATCAAAACTTAAGAATGCATAATCACCATATTATAGACCC
TARGET=50,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7156
PRIMER_LEFT_PENALTY=0.629225
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=GGACCTCAAATTGAAACAACCT
PRIMER_RIGHT_SEQUENCE=CTTTCTTGCATGTGAAGTCAGG
PRIMER_LEFT=7,22
PRIMER_RIGHT=179,22
PRIMER_LEFT_TM=59.371
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.7177
PRIMER_LEFT_1_PENALTY=0.629225
PRIMER_RIGHT_1_PENALTY=0.088435
PRIMER_LEFT_1_SEQUENCE=GGACCTCAAATTGAAACAACCT
PRIMER_RIGHT_1_SEQUENCE=GCAGCAGCAGTATCAAAGTGTC
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=243,22
PRIMER_LEFT_1_TM=59.371
PRIMER_RIGHT_1_TM=60.088
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=0.9082
PRIMER_LEFT_2_PENALTY=0.629225
PRIMER_RIGHT_2_PENALTY=0.278953
PRIMER_LEFT_2_SEQUENCE=GGACCTCAAATTGAAACAACCT
PRIMER_RIGHT_2_SEQUENCE=AAGCAGCAGCAGTATCAAAGTG
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=245,22
PRIMER_LEFT_2_TM=59.371
PRIMER_RIGHT_2_TM=59.721
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=1.0461
PRIMER_LEFT_3_PENALTY=0.629225
PRIMER_RIGHT_3_PENALTY=0.416906
PRIMER_LEFT_3_SEQUENCE=GGACCTCAAATTGAAACAACCT
PRIMER_RIGHT_3_SEQUENCE=CCATAAGCAGCAGCAGTATCAA
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=59.371
PRIMER_RIGHT_3_TM=60.417
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=1.1329
PRIMER_LEFT_4_PENALTY=0.629225
PRIMER_RIGHT_4_PENALTY=0.503688
PRIMER_LEFT_4_SEQUENCE=GGACCTCAAATTGAAACAACCT
PRIMER_RIGHT_4_SEQUENCE=CAGGCATCTTAATTTGAGCAGA
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=137,22
PRIMER_LEFT_4_TM=59.371
PRIMER_RIGHT_4_TM=59.496
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=131
=
PRIMER_SEQUENCE_ID=SSR_03176 Ps_contig_mira-and-tgicl-ass_franssen_29002
SEQUENCE=CTTAAAAGCCCATTATATTCCACAAATCCTTTGATTTCTCTCTGTAACTCTTCTCAGGATCCGGGGCAGCCACAAACCCTAAATCAGCCTGCGCTTCTCTGCTCTTTACTGCCGCCGCAGCCGCTCGCAGTCACCGAGACGAAGTTGCAACCATGGGGCGAGTTCGTACCAAAACCGTGAAGAAATCCTCCCGTCAAGTCATCGAGAGGTACTACTCTCGAATGACTCTGGATTTCCACACCAACAAGAAGATTCTAGAGGAGGTTGCTCTGATTCCAACCAAGAGGCTTCGAAACAAGATTGCAGGATTCTCCACTCACCTTATGAAGCGTATCCAGAAAGGACCGGTTCGCGGAATCTCCCTGAAGCTGCAGGAGGAGGAGCGTGAACGTCGTATGGACTTTGTTCCTGAAGTCTCTGCCATTAGGACTGACCAGATCGAAGTCGATAAGGAAACTCTTGAAATGCTTGCCGCTCTCGGTATGTCTGAGATTCCCGGCGTCGTCCAGGTTGATCCGGTTGCAGTTCAGCCGATCCCCTTTGGTCGCGGTGGTGGCGGCGCTGGTGCTCCTGGAAGGAGGTTTTAAGTTTAAGCTTTTTTCTAATTTTTGTTATGTCAAGACTATTAGATTTGTTTTACTTTTGAAAAATGTTTTTGTTATGGTTGAACTGGTTTAAGTTATTGTAATGGAATTTTAATATATATATTTTTTGCATTCTGAATTGTATGGAATTGTATGAATGATGCTAGTTTTATGACTTTGAATTAATAATCTGTGAGTTGTAAGGTTTAANNGGTTTATGGTGGGTTTTATTAGGGTTCATGAATGGTGGTTTTTTCCATCATAAAGCTCACATCACATTCGTTTTGTAATTGGTTTCATAATTTTGGATTAGTTTTAGAC
TARGET=699,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0238
PRIMER_LEFT_PENALTY=0.580833
PRIMER_RIGHT_PENALTY=0.442993
PRIMER_LEFT_SEQUENCE=GCTCCTGGAAGGAGGTTTTAAG
PRIMER_RIGHT_SEQUENCE=TGAACCCTAATAAAACCCACCA
PRIMER_LEFT=566,22
PRIMER_RIGHT=823,22
PRIMER_LEFT_TM=60.581
PRIMER_RIGHT_TM=60.443
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=1.0437
PRIMER_LEFT_1_PENALTY=0.580833
PRIMER_RIGHT_1_PENALTY=0.462913
PRIMER_LEFT_1_SEQUENCE=GCTCCTGGAAGGAGGTTTTAAG
PRIMER_RIGHT_1_SEQUENCE=AACCACCATTCATGAACCCTAA
PRIMER_LEFT_1=566,22
PRIMER_RIGHT_1=835,22
PRIMER_LEFT_1_TM=60.581
PRIMER_RIGHT_1_TM=60.463
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=1.0437
PRIMER_LEFT_2_PENALTY=0.580833
PRIMER_RIGHT_2_PENALTY=0.462913
PRIMER_LEFT_2_SEQUENCE=GCTCCTGGAAGGAGGTTTTAAG
PRIMER_RIGHT_2_SEQUENCE=AAACCACCATTCATGAACCCTA
PRIMER_LEFT_2=566,22
PRIMER_RIGHT_2=836,22
PRIMER_LEFT_2_TM=60.581
PRIMER_RIGHT_2_TM=60.463
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=1.1162
PRIMER_LEFT_3_PENALTY=0.580833
PRIMER_RIGHT_3_PENALTY=0.535412
PRIMER_LEFT_3_SEQUENCE=GCTCCTGGAAGGAGGTTTTAAG
PRIMER_RIGHT_3_SEQUENCE=ACCACCATTCATGAACCCTAAT
PRIMER_LEFT_3=566,22
PRIMER_RIGHT_3=834,22
PRIMER_LEFT_3_TM=60.581
PRIMER_RIGHT_3_TM=59.465
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=1.8871
PRIMER_LEFT_4_PENALTY=0.580833
PRIMER_RIGHT_4_PENALTY=1.306217
PRIMER_LEFT_4_SEQUENCE=GCTCCTGGAAGGAGGTTTTAAG
PRIMER_RIGHT_4_SEQUENCE=CCACCATTCATGAACCCTAATA
PRIMER_LEFT_4=566,22
PRIMER_RIGHT_4=833,22
PRIMER_LEFT_4_TM=60.581
PRIMER_RIGHT_4_TM=58.694
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=5.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_03177 Ps_contig_mira-and-tgicl-ass_franssen_29006
SEQUENCE=TTTACGNTGTAAGGTTCATAATATATCAAATATGTATGTTAGCATAGTTGCATCCAATCTATGAAAATTTCAAAATCAAACTACTGATAACAATATGTAAGCTACCTGCATAAATAACAGTAAGTTTACTCAATCCACAAAGCAGTTTTTAGACCTTAAAATTTACATTGAACTCATTAAGCAAGCATGTAACACTAAAACAGCAGTTGAAGCGAAGAGAGAACTGTTTTTTCTAGATACATTGTAACCCTCATGTACTCTTTTGCTCGAAGGCTTCCAACTGACTCCGAAGATTCTGATTGTCTTCCAAGAGACGATCATATTCCATAAGGAAACCCTCCGATTGTTTTTTAAGAGCCTCTACCTCCGATTCCAAAGTCTTCTCCTTATTTCCTTTCGCATCACATTCAGATTCCAGCTTCTTGATTTTAGACTTCAATGTGGTAATTTCTTCCGCCAACGCTTTTTGCTCCTCGGTGTTTCCGTTCTTACCATCCTCAAAACTTCGGGTTTGTTTCTTAACTGCTTCCATAGTCTTCCTGAGCAAACGAAGCTCTCTAATGTAGTGATGCAATCTATCAATCATAAGAGATAGAAACAGCACAAAACCCATTAAAGAAGCTTCGAGCATGTGTTTAGACATAAGAACTTGATCAGTAGGGTTAACAATACCAGCTTCAATGGTACGTTGCTGGATCTTAGCCATACTGTAGAGACTAGAAGAAAGCACAACAACTAATGTAGCACCAACGGTGGTAACGACGATTGGACCACGACCTCGTTTGACTCGATCGAGAGAAACGATCACGAGTTTTCTGAGTGGGGTTTTGAAAACGAGGGTTAGGATGAGAAGCATTTCGGCGAAGATCGCAGTGTAGAGTAACTGCAACATGGTGTTGTTGGATGAAGAATGTTGAATCTAGTGTATGATGAGTGTTTTAGTAGCAGAGAAAGATGAAATTGAAGAATAAGAAGAGAAGAGAAGAGAAGAAGCGTGTCGGTGACGAGTTTGTTTGGTGTGCCGGTCCAATAAAGCACGCCAAACCCYCCCCGAC
TARGET=971,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03178 Ps_contig_mira-and-tgicl-ass_franssen_29008
SEQUENCE=CTGCTTATATGTATTCAATATGGCTTGGAGAAGGGTCATCCAAAATGTTAGATTATGGACCTTGCTGGAACGTCCTTTGGTTTATTTGAAGCGAATAATACAGTCGATTTACCGTTTGAAAGAGCCGTGCAAAAGAAGTAATCTTCAGGAAGGAGCAATGAATGCTAAGGCATGGGCTTCCAAGTCCCTTGCCATAGTTTCAAGTTGGAAAGAAAGAAAGCCGCCAACCGNGTAATGCACAGTAGTGCCTGTAGACGTTAAACGTTT
TARGET=208,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1353
PRIMER_LEFT_PENALTY=0.003602
PRIMER_RIGHT_PENALTY=0.131699
PRIMER_LEFT_SEQUENCE=CGTCCTTTGGTTTATTTGAAGC
PRIMER_RIGHT_SEQUENCE=CAGGCACTACTGTGCATTACNC
PRIMER_LEFT=70,22
PRIMER_RIGHT=250,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.132
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.50
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0250
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.75
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.1353
PRIMER_LEFT_1_PENALTY=0.003602
PRIMER_RIGHT_1_PENALTY=0.131699
PRIMER_LEFT_1_SEQUENCE=GTCCTTTGGTTTATTTGAAGCG
PRIMER_RIGHT_1_SEQUENCE=CAGGCACTACTGTGCATTACNC
PRIMER_LEFT_1=71,22
PRIMER_RIGHT_1=250,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=60.132
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.50
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=7.0250
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.3557
PRIMER_LEFT_2_PENALTY=0.224014
PRIMER_RIGHT_2_PENALTY=0.131699
PRIMER_LEFT_2_SEQUENCE=GAAGGAGCAATGAATGCTAAGG
PRIMER_RIGHT_2_SEQUENCE=CAGGCACTACTGTGCATTACNC
PRIMER_LEFT_2=148,22
PRIMER_RIGHT_2=250,22
PRIMER_LEFT_2_TM=60.224
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.50
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=7.0250
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.3557
PRIMER_LEFT_3_PENALTY=0.224014
PRIMER_RIGHT_3_PENALTY=0.131699
PRIMER_LEFT_3_SEQUENCE=GGAAGGAGCAATGAATGCTAAG
PRIMER_RIGHT_3_SEQUENCE=CAGGCACTACTGTGCATTACNC
PRIMER_LEFT_3=147,22
PRIMER_RIGHT_3=250,22
PRIMER_LEFT_3_TM=60.224
PRIMER_RIGHT_3_TM=60.132
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.50
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=7.0250
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_3=104
PRIMER_PAIR_PENALTY_4=0.3557
PRIMER_LEFT_4_PENALTY=0.224014
PRIMER_RIGHT_4_PENALTY=0.131699
PRIMER_LEFT_4_SEQUENCE=AGGAAGGAGCAATGAATGCTAA
PRIMER_RIGHT_4_SEQUENCE=CAGGCACTACTGTGCATTACNC
PRIMER_LEFT_4=146,22
PRIMER_RIGHT_4=250,22
PRIMER_LEFT_4_TM=60.224
PRIMER_RIGHT_4_TM=60.132
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.50
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=7.0250
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_03179 Ps_contig_mira-and-tgicl-ass_franssen_29009
SEQUENCE=TCCACTCAGGTTCAGGCACGTCACATCTTGTTATCTCATTCATCACCCTCTTTCTTTTCTTCTCTTCTCTTCTCATTTTCCCCTTTTCAAATCCAATTAGGGTTTCATTTCCATCTCGAATTTTTTCCTTCTCGTTCCCATTTTTTTCTTCCGTTTTTATATTAATAATCCAAAGGGAAGGGACGAGGATGAGGCTTTGGAGATCGGAATTCGTCCTCGCTATCTTTCTCTGTTGTGGGGCCCTTTCCTCCACCGCCACCGCCACCGCCACCGCCACCGCCGTTTGTCCCACCGAGTCCCTTTTGGATTCCGTTTTAGGGTTTCCAGATTCGACTTGTTCGTTATCAGACTCTCTTGGATCCATTCGTTATGTTGGTGTCACCCAGGGAGATGAGGCTTCTTTGCAGAAGGCACTGAATATGGTTCATAAGAATTATCATGAGTATGTGGCTGTGCTCTTTTATGCATCCTGGTGTCCTTTTTCACGGGTATTTAGACCGGTTTTTTCTGTTCTTTCATCCTTACATCCTTCTATACCTCATTTTGCTATTGAAGAATCATCGGTTAGACCAAGTACGTTATCCAAGTATGGTGTCCATGGTTTTCCTACATTATTCATTTTGAATTCTACTATGCGCGTTCGGTATCATGGTTCTCGGACGCTTGTATCTCTCGTAGGTTTCTACAATGATGTTACTGGAATTAGGATTGATTCACTTGACCAACTATCCCTTGAGAAAATTGGGCGTCCATCAGATGATGAGAATCTAAGCGACACTGAACCAGAAAGTTGTCCATTTTCATGGGCTAGATCTCCAGAAAATTTATTGCGACAGGAAACTTATCTGGCTTTGGCCACTGCATTTGTGGTTTTGAGATTACTCTACCTGTTTTCTCCTACCCTGCTTATATGTATTCAATATGCTTGGAGAAGGGTCATCCAAAATGTTAGATTATGGACCTTGCTGGAACGTCCTTTGGTTTATTTGAAGCGAATAATACAGTCGATTTACCGTTTGAAAGAGCCGTGCAAAAGAAGTAATCTTCAGGAAGGAGCAATGAATGCTAAGGCATGGGCTTCCAAGTCCCTTGCCACAGTTTCAATTGGAGAAGAAAGCACCAGCCGCGTAATGCACCAGTAAGTTGCCTGTAGCGTAAAAGTTTTTTCCCTGTGAAGTATACCCTATTAAATGCTTGGCTGCAAGTTATGCCACGTTCTCTTGTAATCTTCACACCCTTGAGCCTCACGCATAGTGAGAAGTCAATTGTGTTTCTGAATGATGTAATCATCCCTTTTTTGTCCATATGATCAAGAAATACTTCATTCAGCATTCTAGAAAATTGACTTCAATGTGTATAGTTTAAACGGAAAAATTCAGACCTTAAATCTGTAATTCTTTGACTTCTTGTAGGTTTACCTGAAGGTCAAATCTTTTTGATCATCAGTTCATAAATTTAATTTGTTGGGAATGAAGTGATTGTAATCCTACCCTGTAGTACTGTTTGCCTGTCATGTTCATGTACTCAAACATGTTTATTAARACTTAAAAAGTAATAAATCTGATAC
TARGET=58,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03180 Ps_contig_mira-and-tgicl-ass_franssen_29013
SEQUENCE=AAGCAGTGGTAATCAACGTCAGAGTACGGAGGCGATTGCTACTTTCACTTTCTTCCTTCTTTCTCATATCTTCTTCCCAAACTTGAAGATTAACTTTCTCAACATGAAGAAAGTGTTCGTGTTTCTGTCTCTTCTCTTTTGTTCCACTTTCCATGTCTCCCTCTCCTTCAGCGACGGTCTTGTTGCAAATGGGAATTTCGAGCTTGGTCCAAAGCCATCAGAGCTAAAAGGAACAATAGTAACAGGAGGCAAAAACTCAATACCAGAATGGGAAATTTCAGGCTTAGTAGAATACATAAAATCAGGACAAAAACAAGGTGACATGTTACTGGTTGTACCAGAAGGTGCTTATGCAGTGAGATTAGGAAATGAAGCATCCATTAAACAAAGGATTAAAGTGATTAAAGGAATGTATTATTCAATCACTTTTATGGTGGCACGTACTTGTGCACAGGAAGAGAGAATTAATATTTCTGTTGCTCCTGATTTTGGTGTCATTCCTATACAAACTTTGTATACAAGTAGTGGTTGGGATCCAATTGCTTATGGATTCAAAGCTGAGTTTAATGTTGTTGAAATGGTTATTCATAATCCTGGTGTTGAAGAAGATCCTGCTTGTGGTCCTCTTATTGATTCTGTTGCTCTTAGAACTCTTTACCCTCCTAGACCTACTAATAAGAACATATTAAAGAATGGTGGATTTGAAGAAGGACCTTACATAATTCCAAACTCATCCTATGGAGTAATTATCCCACCAAACATAGAAGATGACCATTCACCTCTCCCAGGATGGATGGTAGAGTCCTTAAAAGCAGTGAAGTACCTAGACTCGGACCATTTCTCAGTTCCAGAAGGAACAAGAGCAATAGAGCTAGTAGCAGGTAAAGAAAGCGCAATAGCACAAGTGGCTAGAACAATCCCAGGCAAAACCTATGTTCTCTCATTTTCTGTTGGTGATGCAAGCAATGCATGTGAAGGTTCAATGATTGTTGAAGCATTTGCTGGTAAAGACACTATTAAAGTACCTTATGAATCCAAAGGTAAAGGTGGTTTCAAACGTGCTGCTCTCAAGTTTGTTGCTGCTGGAACAAGAACAAGAGTGATGTTTCTTAGTACTTTTTATAGTATGAGAAGTGATGATCTTTCTTCTCTTTGTGGACCTGTTATTGATGATGTTAAGTTGCTTAGTCTGCGTAAACCATAAGAGGATTGTGAGTTATTTGGGACTGTCATGGTGTGAGTGAGACTATAGTATCAAAATATTATATGTTTGGGTTTGAGACTTTTTATCAGTGGATTTATATATATTTGCTAAGTTTGATTTTTAACTTTAATATATGAAAAAAAGTGTGTATCTCAAATGATGTTAGCAATATAATATATAGACATTCATATGAAAATGTGTGTGAATGTAATGATGGATGGTAGTGTATATGAATATATATAGTATGCAGTAATGTGTTCTATAAATCTCTCATATATATATTTGTACTGATAAAANANAAANAATANATATACC
TARGET=1477,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03181 Ps_contig_mira-and-tgicl-ass_franssen_29018
SEQUENCE=AANAATACAACATTTATATGATATGAACGTATNAAGTATGAACGTTTTCGTAGGGGAGAAGAAAGTTGTCACGAAGAAGGAAAGAATTTAGAGAGAGAAAATTTAAAGTAAAGGAAATTGAGATTGAAAAGCAAAACGTATTATTGCCTCGCTGGAAACGCTATAATTCGATTTTGACTCTCGATAATCGATTTCTAATTTTTCTCTTCTCTTCTTTTTCCTTCTCCCTTTTTCCCCTCTTCGCTTTTTCTTTCTTTCCTCTTCCTTCTCGTTCTCTCTCGCACACAACAATGTCTTACGCATATCTCTTCAAGTATATCATCATCGGAGACACTGGAGTTGGAAAGTCATGTCTTCTACTTCAGTTCACCGACAAGCGCTTTCAACCTGTTCATGACCTGACCATTGGTGTTGAGTTCGGTGCTAGGATGATCACGATTGATAATAAGCCAATCAAGTTGCAAATATGGGATACGGCAGGTCAAGAATCCTTCAGATCTATTACAAGGTCGTATTACAGAGGGGCTGCAGGTGCACTGCTTGTTTATGATATAACCAGGAGGGAGACATTTAATCACTTGGCTAGCTGGTTGGAAGATGCAAGGCAGCATGCAAATGCAAATATGACAATTATGCTGATTGGAAACAAGTGTGATCTTGCTCACAGACGGGCTGTAAGCACAGAAGAAGGTGAACAGTTTGCAAAGGAGCATGGGTTGATCTTCATGGAGGCCTCAGCAAAAACTGCTCAGAATGTTGAAGAGGCCTTCATAAAAACAGCTGGAACCATATACAAAAAGATTCAGGATGGAGTTTTTGATGTATCAAATGAGTCTTATGGAATAAAAGTAGGATATGGTGGAATTCCTGGACCATCTGGGGGTAGGGATGGCCCTTCTGCTGCTGGTGGAAGCTGCTGCAGTTGAAGTGAAACATATCGCAACCTGTTTGTTTCGAACGTAGTTCTTTATGTATTCTCTTCTCTTTTGGGTTTGGATATATGGAAGTATACACATTCGTGGGTTTGATAAAATCAGATAATTGTATAATGGTTTCTTTTGAATCTTTGACTTAAACTGATTATTTGTTTATATTTGTGTGCATAGTTAATTGATTTGTTGTTTCACTCTTCTGTTGAAATATAATTATTGGTATTGTATTAAGCCCCAAGGGCCAGAAATATGTCAAAATGCCATTGATAAGATATTGGCAAAACTCATTTCTGAGGTTTACAGCATCCTATCTGCAGGAGCTAGAGGAACAAGTGAGTCCATCCTACTCAAGTAATTATTAGTTTAAATAGTAGGGGAAATTAAGAGTTGGTTGGGCTAGTTGGTTTTGTTGTTGTTTGAAGTT
TARGET=247,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0163
PRIMER_LEFT_PENALTY=0.003820
PRIMER_RIGHT_PENALTY=0.012470
PRIMER_LEFT_SEQUENCE=GTATTATTGCCTCGCTGGAAAC
PRIMER_RIGHT_SEQUENCE=GTCAGGTCATGAACAGGTTGAA
PRIMER_LEFT=137,22
PRIMER_RIGHT=402,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.2516
PRIMER_LEFT_1_PENALTY=0.239177
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=CTCGCTGGAAACGCTATAATTC
PRIMER_RIGHT_1_SEQUENCE=GTCAGGTCATGAACAGGTTGAA
PRIMER_LEFT_1=147,22
PRIMER_RIGHT_1=402,22
PRIMER_LEFT_1_TM=60.239
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.2639
PRIMER_LEFT_2_PENALTY=0.003820
PRIMER_RIGHT_2_PENALTY=0.260060
PRIMER_LEFT_2_SEQUENCE=GTATTATTGCCTCGCTGGAAAC
PRIMER_RIGHT_2_SEQUENCE=CGAGAGAGAACGAGAAGGAAGA
PRIMER_LEFT_2=137,22
PRIMER_RIGHT_2=280,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=60.260
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=0.3424
PRIMER_LEFT_3_PENALTY=0.239177
PRIMER_RIGHT_3_PENALTY=0.103187
PRIMER_LEFT_3_SEQUENCE=CTCGCTGGAAACGCTATAATTC
PRIMER_RIGHT_3_SEQUENCE=TCAATCGTGATCATCCTAGCAC
PRIMER_LEFT_3=147,22
PRIMER_RIGHT_3=441,22
PRIMER_LEFT_3_TM=60.239
PRIMER_RIGHT_3_TM=60.103
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=0.3598
PRIMER_LEFT_4_PENALTY=0.003820
PRIMER_RIGHT_4_PENALTY=0.355977
PRIMER_LEFT_4_SEQUENCE=GTATTATTGCCTCGCTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=AAGACATTGTTGTGTGCGAGAG
PRIMER_LEFT_4=137,22
PRIMER_RIGHT_4=296,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=60.356
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_03182 Ps_contig_mira-and-tgicl-ass_franssen_29019
SEQUENCE=AACGTTTTCGTAGGGGAGAAGAAAGTTGTCACGAAGAAGGAAAGAATTTAGAGAGAGAAAATTTAAAGTAAAGGAAATTGAGATTGAAAGGCAAAACGTATTATTGCCTCGCTGGAAACGCTATAATTCGATTTTGACTCTCGATAATCGATTTCTTAATTTTTCTCTTCTCTTCTTTTCTCTCCCTTTTCCCTCTTCGCTTTTCTTTCTTTCCTCTTCCTTCTCGTTCTCTCTCGCACACAACAATGTCTTACGCATATCTCTTCAAGTATATCATCATCGGAGACACTGGAGTTGGAAAGTCATGTCTTCTACTTCAGTTCACCGACAAGCGCTTTCAACCTGTTCATGACCTGACCATTGGTGTTGAGTTCGGTGCTAGGATGATCACGATTGATAATAAGCCAATCAAGTTGCAAATANTAGGGATACGGTCAAGAATCCTT
TARGET=202,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0163
PRIMER_LEFT_PENALTY=0.003820
PRIMER_RIGHT_PENALTY=0.012470
PRIMER_LEFT_SEQUENCE=GTATTATTGCCTCGCTGGAAAC
PRIMER_RIGHT_SEQUENCE=GTCAGGTCATGAACAGGTTGAA
PRIMER_LEFT=97,22
PRIMER_RIGHT=357,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.1070
PRIMER_LEFT_1_PENALTY=0.003820
PRIMER_RIGHT_1_PENALTY=0.103187
PRIMER_LEFT_1_SEQUENCE=GTATTATTGCCTCGCTGGAAAC
PRIMER_RIGHT_1_SEQUENCE=TCAATCGTGATCATCCTAGCAC
PRIMER_LEFT_1=97,22
PRIMER_RIGHT_1=396,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.1881
PRIMER_LEFT_2_PENALTY=0.175636
PRIMER_RIGHT_2_PENALTY=0.012470
PRIMER_LEFT_2_SEQUENCE=TCTTTTCTCTCCCTTTTCCCTC
PRIMER_RIGHT_2_SEQUENCE=GTCAGGTCATGAACAGGTTGAA
PRIMER_LEFT_2=173,22
PRIMER_RIGHT_2=357,22
PRIMER_LEFT_2_TM=60.176
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=0.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.2516
PRIMER_LEFT_3_PENALTY=0.239177
PRIMER_RIGHT_3_PENALTY=0.012470
PRIMER_LEFT_3_SEQUENCE=CTCGCTGGAAACGCTATAATTC
PRIMER_RIGHT_3_SEQUENCE=GTCAGGTCATGAACAGGTTGAA
PRIMER_LEFT_3=107,22
PRIMER_RIGHT_3=357,22
PRIMER_LEFT_3_TM=60.239
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=0.2639
PRIMER_LEFT_4_PENALTY=0.003820
PRIMER_RIGHT_4_PENALTY=0.260060
PRIMER_LEFT_4_SEQUENCE=GTATTATTGCCTCGCTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=CGAGAGAGAACGAGAAGGAAGA
PRIMER_LEFT_4=97,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=60.260
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_03183 Ps_contig_mira-and-tgicl-ass_franssen_29020
SEQUENCE=ATCAACGCAGAGTACGCGGGGACAACAACAACAAAAAACAAAAAACACTCAAAAATGTCGAAAGAAGTGAGTGAAGAAGGTCACCAACCTCATCATCATAGTGGAAAAGACTATGTTGACCCACCACCAGCACCTCTTCTCGACTTCGCTGAGATTAAGCTCTGGTCTTTCTACAGAGCTCTCATCGCTGAGTTCATAGCCACTCTCCTCTTCCTCTACGTCACCGTCGCCACTGTCATTGGCCACAAGAAACAAACCGGACCATGCGACGGTGTTGGCCTTCTCGGCATAGCATGGTCCTTCGGTGGCATGATATTTGTCCTTGTCTACTGCACCGCCGGTATCTCCGGTGGCCATATCAACCCTGCCGTCACATTTGGCCTCTTCTTAGCTCGTAAGGTTTCTCTTATAAGAGCTGTTTTATACATTATATCACAGTGCTTAGGTGCTATTTGCGGTGTTGGGTTGGTGAAGGCTTTCATGAAGCACCCTTACAACAGCCTCGGTGGTGGTGCTAACTCTGTTGCTTCTGGTTATACCAAAGGCTCCGCACTTGGTGCTGAAATTATCGGAACTTTTGTGCTTGTTTACACCGTTTTCTCTGCTACTGATCCTAAGAGAAGCGCACGTGACTCGCATATTCCTGTTTTGGCTCCTTTGCCTATTGGTTTTGCTGTTTTCATGGTTCATCTCGCAACTATACCTATCACCGGCACCGGAATTAACCCTGCTAGAAGCTTTGGTGCTGCGGTTATCTTCAACAATGGCAAAGTTTGGGATGACCAATGGATTTTCTGGGTTGGACCGTTCGTCGGAGCGGCGGCGGCGGCTGCGTACCATCAGTATATTCTGAGAGCGGCGGCTATCAAGGCGTTGGGGTCGTTCAGAAGCAATCCAACCAACTAATGATGCATAGATTATGTGTTTATTTGTATGGCTACTATGATGAGATTGATTAATTTGTATTTTACTATTTTTATTTATTTATTATTCGGTTTGTTTGTATGAGATGATGCATGTGATACTCACTTTTCATTTCATATTACTATTTATATTATAATTACAATTTATTATTTACAACCAAT
TARGET=22,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03184 Ps_contig_mira-and-tgicl-ass_franssen_29020
SEQUENCE=ATCAACGCAGAGTACGCGGGGACAACAACAACAAAAAACAAAAAACACTCAAAAATGTCGAAAGAAGTGAGTGAAGAAGGTCACCAACCTCATCATCATAGTGGAAAAGACTATGTTGACCCACCACCAGCACCTCTTCTCGACTTCGCTGAGATTAAGCTCTGGTCTTTCTACAGAGCTCTCATCGCTGAGTTCATAGCCACTCTCCTCTTCCTCTACGTCACCGTCGCCACTGTCATTGGCCACAAGAAACAAACCGGACCATGCGACGGTGTTGGCCTTCTCGGCATAGCATGGTCCTTCGGTGGCATGATATTTGTCCTTGTCTACTGCACCGCCGGTATCTCCGGTGGCCATATCAACCCTGCCGTCACATTTGGCCTCTTCTTAGCTCGTAAGGTTTCTCTTATAAGAGCTGTTTTATACATTATATCACAGTGCTTAGGTGCTATTTGCGGTGTTGGGTTGGTGAAGGCTTTCATGAAGCACCCTTACAACAGCCTCGGTGGTGGTGCTAACTCTGTTGCTTCTGGTTATACCAAAGGCTCCGCACTTGGTGCTGAAATTATCGGAACTTTTGTGCTTGTTTACACCGTTTTCTCTGCTACTGATCCTAAGAGAAGCGCACGTGACTCGCATATTCCTGTTTTGGCTCCTTTGCCTATTGGTTTTGCTGTTTTCATGGTTCATCTCGCAACTATACCTATCACCGGCACCGGAATTAACCCTGCTAGAAGCTTTGGTGCTGCGGTTATCTTCAACAATGGCAAAGTTTGGGATGACCAATGGATTTTCTGGGTTGGACCGTTCGTCGGAGCGGCGGCGGCGGCTGCGTACCATCAGTATATTCTGAGAGCGGCGGCTATCAAGGCGTTGGGGTCGTTCAGAAGCAATCCAACCAACTAATGATGCATAGATTATGTGTTTATTTGTATGGCTACTATGATGAGATTGATTAATTTGTATTTTACTATTTTTATTTATTTATTATTCGGTTTGTTTGTATGAGATGATGCATGTGATACTCACTTTTCATTTCATATTACTATTTATATTATAATTACAATTTATTATTTACAACCAAT
TARGET=817,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2634
PRIMER_LEFT_PENALTY=0.009871
PRIMER_RIGHT_PENALTY=0.253482
PRIMER_LEFT_SEQUENCE=GCCTATTGGTTTTGCTGTTTTC
PRIMER_RIGHT_SEQUENCE=TAGTTGGTTGGATTGCTTCTGA
PRIMER_LEFT=659,22
PRIMER_RIGHT=904,22
PRIMER_LEFT_TM=60.010
PRIMER_RIGHT_TM=59.747
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.3002
PRIMER_LEFT_1_PENALTY=0.046672
PRIMER_RIGHT_1_PENALTY=0.253482
PRIMER_LEFT_1_SEQUENCE=ACTGATCCTAAGAGAAGCGCAC
PRIMER_RIGHT_1_SEQUENCE=TAGTTGGTTGGATTGCTTCTGA
PRIMER_LEFT_1=606,22
PRIMER_RIGHT_1=904,22
PRIMER_LEFT_1_TM=60.047
PRIMER_RIGHT_1_TM=59.747
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=299
PRIMER_PAIR_PENALTY_2=0.3713
PRIMER_LEFT_2_PENALTY=0.117817
PRIMER_RIGHT_2_PENALTY=0.253482
PRIMER_LEFT_2_SEQUENCE=TGCTGTTTTCATGGTTCATCTC
PRIMER_RIGHT_2_SEQUENCE=TAGTTGGTTGGATTGCTTCTGA
PRIMER_LEFT_2=671,22
PRIMER_RIGHT_2=904,22
PRIMER_LEFT_2_TM=60.118
PRIMER_RIGHT_2_TM=59.747
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=234
PRIMER_PAIR_PENALTY_3=0.3844
PRIMER_LEFT_3_PENALTY=0.130883
PRIMER_RIGHT_3_PENALTY=0.253482
PRIMER_LEFT_3_SEQUENCE=TGACTCGCATATTCCTGTTTTG
PRIMER_RIGHT_3_SEQUENCE=TAGTTGGTTGGATTGCTTCTGA
PRIMER_LEFT_3=629,22
PRIMER_RIGHT_3=904,22
PRIMER_LEFT_3_TM=60.131
PRIMER_RIGHT_3_TM=59.747
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.6563
PRIMER_LEFT_4_PENALTY=0.402858
PRIMER_RIGHT_4_PENALTY=0.253482
PRIMER_LEFT_4_SEQUENCE=TCATGGTTCATCTCGCAACTAT
PRIMER_RIGHT_4_SEQUENCE=TAGTTGGTTGGATTGCTTCTGA
PRIMER_LEFT_4=679,22
PRIMER_RIGHT_4=904,22
PRIMER_LEFT_4_TM=59.597
PRIMER_RIGHT_4_TM=59.747
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_03185 Ps_contig_mira-and-tgicl-ass_franssen_29020
SEQUENCE=ATCAACGCAGAGTACGCGGGGACAACAACAACAAAAAACAAAAAACACTCAAAAATGTCGAAAGAAGTGAGTGAAGAAGGTCACCAACCTCATCATCATAGTGGAAAAGACTATGTTGACCCACCACCAGCACCTCTTCTCGACTTCGCTGAGATTAAGCTCTGGTCTTTCTACAGAGCTCTCATCGCTGAGTTCATAGCCACTCTCCTCTTCCTCTACGTCACCGTCGCCACTGTCATTGGCCACAAGAAACAAACCGGACCATGCGACGGTGTTGGCCTTCTCGGCATAGCATGGTCCTTCGGTGGCATGATATTTGTCCTTGTCTACTGCACCGCCGGTATCTCCGGTGGCCATATCAACCCTGCCGTCACATTTGGCCTCTTCTTAGCTCGTAAGGTTTCTCTTATAAGAGCTGTTTTATACATTATATCACAGTGCTTAGGTGCTATTTGCGGTGTTGGGTTGGTGAAGGCTTTCATGAAGCACCCTTACAACAGCCTCGGTGGTGGTGCTAACTCTGTTGCTTCTGGTTATACCAAAGGCTCCGCACTTGGTGCTGAAATTATCGGAACTTTTGTGCTTGTTTACACCGTTTTCTCTGCTACTGATCCTAAGAGAAGCGCACGTGACTCGCATATTCCTGTTTTGGCTCCTTTGCCTATTGGTTTTGCTGTTTTCATGGTTCATCTCGCAACTATACCTATCACCGGCACCGGAATTAACCCTGCTAGAAGCTTTGGTGCTGCGGTTATCTTCAACAATGGCAAAGTTTGGGATGACCAATGGATTTTCTGGGTTGGACCGTTCGTCGGAGCGGCGGCGGCGGCTGCGTACCATCAGTATATTCTGAGAGCGGCGGCTATCAAGGCGTTGGGGTCGTTCAGAAGCAATCCAACCAACTAATGATGCATAGATTATGTGTTTATTTGTATGGCTACTATGATGAGATTGATTAATTTGTATTTTACTATTTTTATTTATTTATTATTCGGTTTGTTTGTATGAGATGATGCATGTGATACTCACTTTTCATTTCATATTACTATTTATATTATAATTACAATTTATTATTTACAACCAAT
TARGET=976,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.6872
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=3.433715
PRIMER_LEFT_SEQUENCE=TCAGAAGCAATCCAACCAACTA
PRIMER_RIGHT_SEQUENCE=AAGTGAGTATCACATGCATCATCTC
PRIMER_LEFT=883,22
PRIMER_RIGHT=1030,25
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=59.566
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=4.0265
PRIMER_LEFT_1_PENALTY=0.253482
PRIMER_RIGHT_1_PENALTY=3.773038
PRIMER_LEFT_1_SEQUENCE=TCAGAAGCAATCCAACCAACTA
PRIMER_RIGHT_1_SEQUENCE=AGTGAGTATCACATGCATCATCTC
PRIMER_LEFT_1=883,22
PRIMER_RIGHT_1=1029,24
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=58.227
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=4.1056
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=3.852135
PRIMER_LEFT_2_SEQUENCE=TCAGAAGCAATCCAACCAACTA
PRIMER_RIGHT_2_SEQUENCE=GTGAGTATCACATGCATCATCTC
PRIMER_LEFT_2=883,22
PRIMER_RIGHT_2=1028,23
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=57.148
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=4.1668
PRIMER_LEFT_3_PENALTY=0.733091
PRIMER_RIGHT_3_PENALTY=3.433715
PRIMER_LEFT_3_SEQUENCE=CAGAAGCAATCCAACCAACTAA
PRIMER_RIGHT_3_SEQUENCE=AAGTGAGTATCACATGCATCATCTC
PRIMER_LEFT_3=884,22
PRIMER_RIGHT_3=1030,25
PRIMER_LEFT_3_TM=59.267
PRIMER_RIGHT_3_TM=59.566
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=4.3281
PRIMER_LEFT_4_PENALTY=0.894350
PRIMER_RIGHT_4_PENALTY=3.433715
PRIMER_LEFT_4_SEQUENCE=GTTCAGAAGCAATCCAACCAAC
PRIMER_RIGHT_4_SEQUENCE=AAGTGAGTATCACATGCATCATCTC
PRIMER_LEFT_4=881,22
PRIMER_RIGHT_4=1030,25
PRIMER_LEFT_4_TM=60.894
PRIMER_RIGHT_4_TM=59.566
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_03186 Ps_contig_mira-and-tgicl-ass_franssen_29036
SEQUENCE=GTCGGGGGGATCTCCACAAAGTGTCAGTGATTCACTTCACTTCACTTCACTAGTGTTGTTGTCGTTGTTTGAAGAAGATGATGTTGGATTCACAACATCATGATTGGTTATATTTCCTGTTCTTCTTCTCTTTCTTACTTGTTTCTAGTTCAGTCAATGGAATTGCAGTACCTGATAATGGAGGGTTAAGTAGACAAGTGTTTCCTAAAGGGTTTGTGTTTGGAGTTGCAACTTCAGCTTATCAAGTTGAGGGTATGGCCAACAAAGATGGTCGTGGCCCTAGTATTTGGGATGTTTTCATCAAAAAACCAGGAATTGTGGCAAATAATGGCACAGGAGATGTTTCTGTTGATCAGTACCATCGCTACAAAGAAGATATTGATCTCATGGCAGAGCTGAATTTTGATGCTTACCGGTTTTCAATCTCATGGTCCAGAATTTTTCCAAATGGAACTGGTAAAGTAAATTGGAAAGGTGTTGCATACTACAACAGATTAATTGATTACCTGCTAGAAAAAGGAATTACTCCATATGCAAATCTGTACCATTATGATCTTCCTTTAGCTCTTGAGTTGAGATACAATGGCTTATTGAGCCATAATGTTGTGAAAGATTTTGCGGATTATGCAGAATTTTGTTTCAAGACATTTGGAGATAGAGTTAAGAACTGGATGACTTTTAATGAACCGAGAGTCGTTGCGGCTCTTGGTTATGACAATGGCTTTTTTGCCCCTGGAAGGTGTTCTAAAGAATATGGAAATTGTACTGCTGGAAATTCAGGAACTGAGCCTTACATTGCAGCTCATAATTTGATATTGTCGCATGCTGCTGCTGTTCAAAGATACAGAGAAAACTACCAAGAAAAGCAAAAGGGTAGGATTGGAATTTTGTTGGATTTTGTTTGGTATGAGCCTCTTACAAGATCAAAGGCTGATAGCTATGCTGCTCAAAGAGCTAGAGACTTTCATATTGGGTGGTTCATTCATCCCATTGTATACGGCGAGTATCCAAAAACTATGCAAAATATTGTTGGGAAGAGGCTCCCAAAATTCACTAAAGAAGAAGTGAAAATTGTGAAGGGTTCCATTGACTTTGTTGGTATCAATCAATACACTACCTACTACATGTATGATCCTCATAAATCAAAACCAAAAGTTCCTGGCTATCAAAATGATTGGAATGTAGGATTTGCTTATGCTAAGAATGGAGTACCTGTTGGACCAAGGGCATATTCATATTGGTTGTACAATGTACCATGGGGCTTGTACAAATCATTGATTTACATCAAGGAACGTTACGGAAATCCAATTGGGATACTATCGATTGTAGGCATGGATGATCCGGGTAATGTGACATTTGCCAAAGGCTTACATGACACGACGAGAATAAATTATTACAAAGGGTATTTGACTCAACTAAAGAAAGCTGTTGATGATGGAGCAAATGTGGTTGGATACCATGCATGGTCATTACTTGATAACTTCGAATGGAGATTAGGGTACACGTCAAGGTTCGGTATTGTCTATGTGGACTTCAAAACTCTCAAGAGAACCCCTAAATTGTCAGCGTATTGGTTCAAGAAACTACTCACCAGAAAGAAGTAAGTTTGTGTTTGAATTCACAATGAGTTTGACAAAATCACAATCAACTATCGTGTAGATTTTGTCAAAACTCGTCGTGTTCCTACTATGGTAGTGAATAATGTTTTTGTTGTTTTATTTTCATCAATTGTAACAATCATGCGAATCGATTAGTGCAAGTCTTGGAATAAAATGATCTTTTCAATAAAGCTTCATGAGTTTAGATTCACT
TARGET=31,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4727
PRIMER_LEFT_PENALTY=0.414033
PRIMER_RIGHT_PENALTY=0.058624
PRIMER_LEFT_SEQUENCE=GGGATCTCCACAAAGTGTCAGT
PRIMER_RIGHT_SEQUENCE=CCAATCATGATGTTGTGAATCC
PRIMER_LEFT=6,22
PRIMER_RIGHT=106,22
PRIMER_LEFT_TM=60.414
PRIMER_RIGHT_TM=60.059
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=0.5818
PRIMER_LEFT_1_PENALTY=0.377832
PRIMER_RIGHT_1_PENALTY=0.203971
PRIMER_LEFT_1_SEQUENCE=GGATCTCCACAAAGTGTCAGTG
PRIMER_RIGHT_1_SEQUENCE=GGCCATACCCTCAACTTGATAA
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=259,22
PRIMER_LEFT_1_TM=59.622
PRIMER_RIGHT_1_TM=60.204
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.6180
PRIMER_LEFT_2_PENALTY=0.414033
PRIMER_RIGHT_2_PENALTY=0.203971
PRIMER_LEFT_2_SEQUENCE=GGGATCTCCACAAAGTGTCAGT
PRIMER_RIGHT_2_SEQUENCE=GGCCATACCCTCAACTTGATAA
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=60.414
PRIMER_RIGHT_2_TM=60.204
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=0.8791
PRIMER_LEFT_3_PENALTY=0.377832
PRIMER_RIGHT_3_PENALTY=0.501224
PRIMER_LEFT_3_SEQUENCE=GGATCTCCACAAAGTGTCAGTG
PRIMER_RIGHT_3_SEQUENCE=GGTACTGCAATTCCATTGACTG
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=171,22
PRIMER_LEFT_3_TM=59.622
PRIMER_RIGHT_3_TM=59.499
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=1.0028
PRIMER_LEFT_4_PENALTY=0.377832
PRIMER_RIGHT_4_PENALTY=0.624930
PRIMER_LEFT_4_SEQUENCE=GGATCTCCACAAAGTGTCAGTG
PRIMER_RIGHT_4_SEQUENCE=ACCCTCCATTATCAGGTACTGC
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=59.622
PRIMER_RIGHT_4_TM=59.375
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_03187 Ps_contig_mira-and-tgicl-ass_franssen_29045
SEQUENCE=TATTNATAGTAGTATCGGCTTACACGNANGNAGGGCATTTTCCTTAACGAGTAAGACACCGCCACTCCGTGTCCGACTCTGAGTCGGCGTGCGAGCCAACCAAACTTAAACAACTCTGCGTGCTCTCAAATTTCAATCAAACAACTCCAATACCAATTTCTCAACTCAATCCAATTCAATTCAATTTCAATGGCGAAACTTGAAGCTAAGCTAGGAATGATCGTTGACAAAGTCGGTCAATTCTTCTCCGGCTCCGACCATCTCCCTTCCTGCGATTCCGACATCATCGCCGGATGTGAAAGAGAGGTTGCTGAGGCCGAGAAACAACCCTCCTCTGATAAGTCTATGCAAGAATCTCTCTTGCGTTTATCATGGGCTCTTGTCCACTCAAAACGTCCAGAAGATGTGCAGCGTGGGATAGCAATGCTTCAATCATCATTACCTAGCACAGTAGATCCGCTGCAGCAAAGGGAGAATCTTTATCTTCTGGCGGTTGGATATTATAGAAGTGGTGACTATTCGAAGAGTAGGGATCTTGTTGAGAAATGCCTTGAGATTGCACCTGACTGGAGGCAGGCATTGACACTTGAGTCAGCGATTCAGGAACGGATTACAAAAGATGGTGTCATTGGCTTAGGCATTGCTGCAACTGTGGCTGCAACTGCCGTTGGACTGATTGCTGGTGGGATTGCAGCAGCCGCATCTCGTAAAAGGTGACAGAATCGTTTCTATTAAGCGTATGATCGCAGATGATGTGTAAGAAGAAAACATATATGGTACTGTGATTTAGCTGCATCTTACTGCTTTTAAAGAATGGAGCAAACAATGATGTGTACAAAGTGGTGGATAGTTTAATATCAAAGTACTAGATAAGAGATATGATGTGTGTTTTCATGTTTTAAGAAATTCTATATTTGTGTTTGTGTTTGCTTGTTTCTCAATCAATTTTTCTCCATCTTTCTGCTAAGCTCCATGAAATATGATGTATGAATAGCCATATCAGTAATTGCAAGTTAAACATTGGACAATTAAGACGTTGATCTGAAGGTTGAATCCAATATTCTGTTTTTAAAANAAAAAACATATCATACCTATAGGAAATCTGGGAAATATTAGTACAATAATTTGGCATCTCAGATCAAGTGTCATCTGTCAATACTAGTGGCAGGGAGT
TARGET=172,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3825
PRIMER_LEFT_PENALTY=0.301100
PRIMER_RIGHT_PENALTY=0.081412
PRIMER_LEFT_SEQUENCE=TTAAACAACTCTGCGTGCTCTC
PRIMER_RIGHT_SEQUENCE=CTTAGCTTCAAGTTTCGCCATT
PRIMER_LEFT=105,22
PRIMER_RIGHT=209,22
PRIMER_LEFT_TM=59.699
PRIMER_RIGHT_TM=59.919
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=105
PRIMER_PAIR_PENALTY_1=0.4106
PRIMER_LEFT_1_PENALTY=0.301100
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=TTAAACAACTCTGCGTGCTCTC
PRIMER_RIGHT_1_SEQUENCE=GGAGAAGAATTGACCGACTTTG
PRIMER_LEFT_1=105,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=59.699
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=0.4679
PRIMER_LEFT_2_PENALTY=0.301100
PRIMER_RIGHT_2_PENALTY=0.166752
PRIMER_LEFT_2_SEQUENCE=TTAAACAACTCTGCGTGCTCTC
PRIMER_RIGHT_2_SEQUENCE=TAGCTTAGCTTCAAGTTTCGCC
PRIMER_LEFT_2=105,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=59.699
PRIMER_RIGHT_2_TM=60.167
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.5476
PRIMER_LEFT_3_PENALTY=0.466172
PRIMER_RIGHT_3_PENALTY=0.081412
PRIMER_LEFT_3_SEQUENCE=NAGGGCATTTTCCTTAACGAGT
PRIMER_RIGHT_3_SEQUENCE=CTTAGCTTCAAGTTTCGCCATT
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=209,22
PRIMER_LEFT_3_TM=60.466
PRIMER_RIGHT_3_TM=59.919
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.5757
PRIMER_LEFT_4_PENALTY=0.466172
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=NAGGGCATTTTCCTTAACGAGT
PRIMER_RIGHT_4_SEQUENCE=GGAGAAGAATTGACCGACTTTG
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=248,22
PRIMER_LEFT_4_TM=60.466
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_03188 Ps_contig_mira-and-tgicl-ass_franssen_29046
SEQUENCE=GCTTAGACTTATCAGAGGAGGGTTGTTTTTCGGCCTCAGCAACCTCTCTTTCACATCCGGCGATGATGTCGGAATCGCAGGAGGGGAGGTGGTCGGAGCCGGAGAAGAATTGACCGACTTTGTCAACGATCATTCCTAGCTTAGCTTCAAGTTTCGCCATCGAAATTGAATTGGATTGGATTGGATTGAGTTGAGAAATTGGTATTGGAGTTGTTTGATTGAAATTTGAGAGCACGCAGACTTGTTTAAGTTTTGTTGGCTCGCACGCCGACTCAGAGTCGGACACGGAGTGGCGGTGTCTTACTCGTTAAGGAAAATGCNNYCGACGNACTCTGCGTTGAT
TARGET=170,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03189 Ps_contig_mira-and-tgicl-ass_franssen_29049
SEQUENCE=AAAAGGGTTTTTTCATTTCCAATTCTCTCTCTCTCTCTCAAGAGATAACGTTAACAATACACACAAAAATGGATCCTGTTCTTAGCAGAAGCATGACAACCGCCTCTCTCGCCACCCTCCCTTCTTCCTCCTCCTTTACACTCTCCAGATCTTCTCCCGCTCTCAGAACTGCCTTTCTCCCCCCTCAACCCCCTCGCAGAAAGTGCTTCTCTTCCAACGCAGCCGCGTTGAAGTGTAAGCACCATGATAGAAGACGAAACGGAAGATTCTCCGTGCGTTGTGAGGCTGCTGTTGCAGAGCCAGAAGAGGCCACCGGCGAGAAGTTTGAATACCAAGCTGAGGTGAGCCGGTTGATGGATTTGATTGTTCACAGTCTTTATAGTCACAAGGAGGTTTTTCTTCGAGAACTTGTGAGCAATGCTAGTGATGCTTTGGACAAGTTGAGGTTTTTAAGTGTGACAGAGCCTTCTTTGCTTGGAGAGGCTGGAGAGCTAGAGATACGCATCAAGGCCGATCCGGACAACGGAACTATTACTATAACAGATACTGGTATTGGAATGACCAAAGAAGAGCTCATTGATTGTCTTGGAACTATTGCGCAGAGTGGTACCTCAAAGTTTTTGAAGGCACTTAAGGAAAACAAGGATTTAGGAGCAGATAATGGTTTGATTGGTCAATTTGGTGTTGGGTTCTACTCAGCATTTCTTGTTGCTGATAAGGTTGTTGTTTCTACAAAGAGCCCCAGATCAGATAAGCAATATGTATGGGAAGCTGCAGCTGACAGCAGCTCTTATGTGATAAAGGAAGAAACTGACCCCGAAAAGCTTCTGCGCCGTGGTACTCAGATTACACTTCACTTAAGACCGGATGACAAGTATGAATTCTCAGAGCCAACCAAAATTCAGAGTTTGGTGAAGAATTATTCGCAGTTTGTTTCCTTCCCCATCTATACATGGCAAGAAAAATCTAGAACCGTGGAGGTGGAAGAGGAAGAAGAACCTAAAGAAGGGGAAGAACCAAAAGCAGAGGGTGATGTAAAGAAGACAAAAAAGACAAAAACTGAGAAGTTTTGGGACTGGGAGTTAGGCAACGAGACAAAGCCAATATGGATGCGTAATTCAAAGGATACTCAAAAGGAAGAGTATGACGAGTTTTACAAGAAGGCTTTTAATGAATTCTTAGAACCACTTGCATATACTCACTTCACTACTGAGGGTGAGGTTGAGTTCAGAAGCATTCTATATATTCCAAGTATGGGACCTCTCAACAATGAGGAAGTTATGAACCCTAAAACAAAGAATATACGCCTGTATGTAAAGCGTGTGTTCATCTCAGATGATTTTGATGGTGAACTGTTTCCGCGTTACCTGAGCTTTGTGAAAGGTGTGGTTGATTCAAATGACCTTCCTCTGAATGTTTCCAGAGAGATCCTTCAGGAAAGCAGAATTGTAAGGATAATGAGAAAAAGACTTGTCAGGAAGACATTTGATATGATTCAAGACCTTTCTGAAAGGGAAAATAAGGAGGATTACAAGAAATTCTGGGAGAACTTTGGTAGGTTCATTAAATTGGGATGCATTGAGGACAGCGGAAATCATAAGCGCATAACACCGCTTTTGAGGTTTTTCACTTCCAAAAGTGAAGAGGAACTTAAAAGCCTAGATGAGTATATTGAAAACATGGGTGAAAACCAAAAGGCTATCTATTATCTAGCAACTGACAGCTTGAGAAGTGCAAAGACTGCTCCGTTTTTAGAGAAATTGGTTCAAAAAGATATTGAGGTTCTTTATTTGGTTGATCCAATTGATGAGGTTGCTATTCAGAACCTACAGACATACAAAGAAAAGAAATTTGTTGATATCAGCAAGGAAGATTTGGAACTGGGTGATGAGGATGAAGTCAAAGACAGGGAAAACAAGCAAGAAAACACTCTACTTTGTGATTGGATAAAACAACAGCTCGGTGATAACGTGGCAAAAGTTCAAGTCTCTAATCGTTTAAGTTCATCTCCTTGTGTACTTGTATCTGGCAAGTTTGGTTGGTCCGCAAATATGGAAAGATTAATGAAGGCACAGGCTCTTGGAGACCAGTCAAGTTTGGAGTTTATGAGAGGGAGGAGAATATTGGAGATTAATCCAGATCACCCCATTATCAAAGATTTAAGTGCTGCCTGCAAAAATGCCCCTGACAGCAATGAAGCTAAGATAGCCGTAGACCTCTTATACGATACAGCACTGATTTCAAGTGGATTCTCTCCGGAGAGCCCTGCCGAGTTGGGAAATAAAATTTATGGAATGATGGCGTTGGCCTTGGGAGGAAGATGGGGTAGAACAGAACGGGAGGGAGATGCACCAGCCGAGGGTGCTGCTGATTCGAATGAAAGTGAAGGTACAGTGACTGAAGTTTTTGAACCATCTGAAGTTATAGCCGAAAATGATCCATGGACAACAGATTAAGTGTCTTTTCCCTCTCAGCTCTCAAATAGCATAAATTAGTTATAGTTTTTGGCAGTATAGTGGTGAGTGGTGAGTGGTGAGTCATACTGAGGCAATAGGAATGGGAGGCCCTGAATCTTGATATGATTTCATACTGCTGAAAGGAAAGTTGCCATAAAGGAGTTTAATCCTATGGGCACTTTCCCTATAAATTTATGATCCAAGTGTTTGATTAGTTTAGAACATTTTTGTTACTTGGAGGTGTTATTCTATCATTTTTTAAGGTGTAGAATGTTTTATTGAAATTTGATGAATTTAATAAGTATTTTAAGTAAANAAAGTTGTAATTTAAGTTTAAAATATACTACAGTGACGTAAATTATTTAG
TARGET=24,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03190 Ps_contig_mira-and-tgicl-ass_franssen_29074
SEQUENCE=CGTTCAAGAATCTTCGAGCGACGAAGAAGATTCCAACCGTCAAAGAATCATCTTGGCGAACCCTTCTACACAGCGCATGATCGTGATCGAAGGAGTTTCAAGTCTGGAAACTCTGTTTCAGGAACTTGAAAGTACTAGAAAGAAGGGTCAGCCACCAGCTTCAAAAGGAGTCGATAGAAGCTATGAAGAGGGTTGAGATTGAAGAAAGTGATCACGGAGAGTGTGTTGTTTGTTTGGAGGAGTTTGAGGTTGGTGGGGTTGTTAGAGAAATGCCATGTGAGCATAGGTTTCATGGGACCTGTATTGAGAAGTGGTTAGGGATTCATGGGTCTTGTCCTGTTTGTAGGTATCAGATGCCTGTTGATGAGAAGGACGAGGGAAAGAAAATTGGGGAAGATGAAGAAGAAGAAGGTGGAGAGAGAGGGAGGGTTGGTGGTGAAGTTTGGGTTAGTTTTTCTATTAGTAGGAGAAGGGGAAATCGTGATCAAAATCAAGCTACTTCTGGTGGTGATTCAATTGATTCTTCATCAAGCTCTAGAGATGGTGATGAGTTTGGGAATTGATCTTGATTTTTGTTTTGTTAGGATTCTGGTTTTCAATTTGTTGATGTAAATGTATAGGCTCAAATGCACAAATTTGTTTTTACATGTGGGAATAAATAATGATATTTTGATTAATT
TARGET=399,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0414
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.029253
PRIMER_LEFT_SEQUENCE=GTTTGTTTGGAGGAGTTTGAGG
PRIMER_RIGHT_SEQUENCE=TCAATTGAATCACCACCAGAAG
PRIMER_LEFT=227,22
PRIMER_RIGHT=519,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.0414
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.029253
PRIMER_LEFT_1_SEQUENCE=TTGTTTGGAGGAGTTTGAGGTT
PRIMER_RIGHT_1_SEQUENCE=TCAATTGAATCACCACCAGAAG
PRIMER_LEFT_1=229,22
PRIMER_RIGHT_1=519,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=59.971
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=291
PRIMER_PAIR_PENALTY_2=0.0414
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=0.029253
PRIMER_LEFT_2_SEQUENCE=TTTGTTTGGAGGAGTTTGAGGT
PRIMER_RIGHT_2_SEQUENCE=TCAATTGAATCACCACCAGAAG
PRIMER_LEFT_2=228,22
PRIMER_RIGHT_2=519,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=59.971
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=1.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=292
PRIMER_PAIR_PENALTY_3=0.0672
PRIMER_LEFT_3_PENALTY=0.037936
PRIMER_RIGHT_3_PENALTY=0.029253
PRIMER_LEFT_3_SEQUENCE=GGTATCAGATGCCTGTTGATGA
PRIMER_RIGHT_3_SEQUENCE=TCAATTGAATCACCACCAGAAG
PRIMER_LEFT_3=345,22
PRIMER_RIGHT_3=519,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=59.971
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.1379
PRIMER_LEFT_4_PENALTY=0.108684
PRIMER_RIGHT_4_PENALTY=0.029253
PRIMER_LEFT_4_SEQUENCE=CATGGGTCTTGTCCTGTTTGTA
PRIMER_RIGHT_4_SEQUENCE=TCAATTGAATCACCACCAGAAG
PRIMER_LEFT_4=323,22
PRIMER_RIGHT_4=519,22
PRIMER_LEFT_4_TM=59.891
PRIMER_RIGHT_4_TM=59.971
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=197
=
PRIMER_SEQUENCE_ID=SSR_03191 Ps_contig_mira-and-tgicl-ass_franssen_29081
SEQUENCE=TCGGGGGAGGAAATGGCGTGAATGAAATGAAGGAATGTGAATACCAAAAGACTCTGATCCTCATTCATTCCTAGGTTGTTCAGTAGAGTGCAAAACCGTTAGCCATGTCTCCAGGTCCCATTGTTGACGCAACCGCCGACGGAACCGCCGTCCAACAACTCCCCTCCGTCGATGAAACAACCCTCAAGAAGGCACATCTTCCCCAGGAAGAAAACGACGCTCCTATTGTCGAGGATGACGACGACGACGAGAAAGACGAATCCGATGACGAGGAAGATGACGCCGCTCAAGGTGGTACTGAAGGTTCAAAGCAGAGCAGAAGTGAGAAGAAGAGTCGCAAAGCAATGTTGAAGCTGGGACTAAAACCAATTGATGGTGTTAGCAGGGTAACAATCAAGAGAACAAAAAATATAATGTTTTTCATCTCAAAACCTGATGTCTTCAAGAGTCCAAACTCTGAGACCTACGTTATATTTGGGGAGGCAAAAATAGAGGACTTGAGCTCTCAATTGCAGACACAAGCTGCTCAACAGTTCCGGATGCCAGATATGGGATCTATTATGGCAAAACAAGAACAGGGTGCTGCAGCTGATAGTGCACAGCCTGAAGAGGAAGAGGAGGTTGATGAAACTGGCGTGGAGGCCCACGATATTGATCTGGTCATGACACAAGCAGGAGTGTCAAGAAGCAAGGCCGTCAAGGCTCTCAAGACTCACAATGGAGACATTGTTGGTGCCATCATGGAGCTAACTAATTGATTTTGCTTTATGCTTTATATTTATTTGCATTTTTGGGATGATTTTGGAACTAGTTCCATCCTTAAGTGAGTGTAGTCGAATAATACTATGGTCCATATGATTTGGTTTACCTAATAATTTTTGTTGTGCTCTGAATTTTGTGCTACGGCTTGATGATTAGTGATCACATTCTTTTGTTTGTTATATTTGAGCTTTCTTAAATTTTTTTTAAGTTTTTACTAAA
TARGET=237,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1898
PRIMER_LEFT_PENALTY=0.189072
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=GTAGAGTGCAAAACCGTTAGCC
PRIMER_RIGHT_SEQUENCE=ATTGGTTTTAGTCCCAGCTTCA
PRIMER_LEFT=82,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=60.189
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.2116
PRIMER_LEFT_1_PENALTY=0.210886
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=AGAAAACGACGCTCCTATTGTC
PRIMER_RIGHT_1_SEQUENCE=ATTGGTTTTAGTCCCAGCTTCA
PRIMER_LEFT_1=208,22
PRIMER_RIGHT_1=369,22
PRIMER_LEFT_1_TM=59.789
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.2290
PRIMER_LEFT_2_PENALTY=0.189072
PRIMER_RIGHT_2_PENALTY=0.039879
PRIMER_LEFT_2_SEQUENCE=GTAGAGTGCAAAACCGTTAGCC
PRIMER_RIGHT_2_SEQUENCE=GCTTTGAACCTTCAGTACCACC
PRIMER_LEFT_2=82,22
PRIMER_RIGHT_2=311,22
PRIMER_LEFT_2_TM=60.189
PRIMER_RIGHT_2_TM=60.040
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.2395
PRIMER_LEFT_3_PENALTY=0.210886
PRIMER_RIGHT_3_PENALTY=0.028588
PRIMER_LEFT_3_SEQUENCE=AGAAAACGACGCTCCTATTGTC
PRIMER_RIGHT_3_SEQUENCE=AAGTCCTCTATTTTTGCCTCCC
PRIMER_LEFT_3=208,22
PRIMER_RIGHT_3=498,22
PRIMER_LEFT_3_TM=59.789
PRIMER_RIGHT_3_TM=59.971
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.2508
PRIMER_LEFT_4_PENALTY=0.210886
PRIMER_RIGHT_4_PENALTY=0.039879
PRIMER_LEFT_4_SEQUENCE=AGAAAACGACGCTCCTATTGTC
PRIMER_RIGHT_4_SEQUENCE=GCTTTGAACCTTCAGTACCACC
PRIMER_LEFT_4=208,22
PRIMER_RIGHT_4=311,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=60.040
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=104
=
PRIMER_SEQUENCE_ID=SSR_03192 Ps_contig_mira-and-tgicl-ass_franssen_29083
SEQUENCE=GTAGATACTNNAAAAAGTACTAAACCTATTTTTTTAGTCACAAACTTAGAACAAGTTCATTAACAGTCACAAAATGTCTTCACAGAAGATAAATACCGATAGAGTTGTAAAACTTCTATAGCTTACAGTTCTAAGTTTGGCTAGGGAATACAAAACTAACCAATGCATACAATACAATACAAAAAGACAATACATCTTACTGCAAGAACTCTCTTGGATCAGTAACGTAACCGGTCAATGCAGATGCGGCAGCTGTATACGGGGAAGCCAAATATATTTCACCTTCCCTGTGGCCCATTCGCCCTGGAAAGTTTCTATTCGTAGTTGAAACACAAACCTGAGGTTCATTCATGCGAGCATAAGTATCTTTCGGGCCACCCAAACAAGCACCACAACTAGGACTAGCAGGTGTGTCGCAACCAGCTTCTTCAAATATCTGTGAGCAAGTCTTACCACCTGAACCGGGGACAGGGAGGCTGTACACATCCATCCAAACCTTCTGTGTTGCCGGGACAAGAAACGTCGGAACTTTAACCTTTTTACCCGATGCTAGAAAAACTTTTGCGGCAGCCATGAAATCTTCTGTTTTCCCACCCGTACAAGATCCTATGTATACTCTGTCAATTTTCACATCCTTGCACTCCCTTGCCAAAGCTCGGTTATCCGGTGAATGAGGCTTGGCCACCAGTGGCTCCAACTTTGTGACATCAAATCTGTACTCAGAAAGAAATCTTGCTTGCTGGTCACTATAAACTGGTTCATATGGCACAGATGTCTTGCCCTCAAGATATTTATATGTAATGCTGTCAGGAGGAATAACACCGTTCTTTCCTCCAGCTTCAACTACCATGTTGCACAATGTCATTCGTTCTTCCATAGTTAAACTTTCAACGGTTGAGCCAACAAACTCCATAGATTTGTAAGTTGCACCGGCCACAGATATTTCACCAATAATTTGCAGAATTAAATCCTTTGCAAGCAAATAACTTGGCATTTCTCCATCCAATACAAATCTCAGAGTTGGAGGCACCTTGAGCAAAAGCTTCCCTGCTCCCAACACAAAACCAGCATCGGTATTACCAATCCCTGTTGCAAATTGACCAAATGCTCCAGCAGTACAAGTGTGAGAATCAGTGCCCAAGAGAACCTCTCCTGGCCTACAATGACCTTCCTGAGCAAGAGCGATGTGACAAACACCTTTGTAATCTGGATTTACCTTAAAATTACTAAGATCCTTAATATCATAAAAGTACTTGATATTTTGCTCCTGACAAAAATCCCTGAGTATGTCTACATTGCGATTGGCACGTTCATCACTTGTGAATATATAGTGATCAGGTATTATTACAAGCTTTTCACGATCCCAAACCTTAGCATCCTCACCAAACTCTTTCTTAAAAATCCCAATAGAACCAGGACCACAGACATCATGTGTCATCAAAACATCAACATTGACCCAAATATTATCCCCAGGTATGGCCTTCTCCTTCTCAGCAGCTCTAGCCAATATCTTCTCAGTCATGGTCATGGCTGTCTTCACTGATCCAGTGGTAGAAGGCTGGCGCTGTGTTTGCTGAGGCGCGGTAACTGAACAAGAGATTCTCCTGGAAGCGTGTTTCTGACATCGCTGAGAAGATGATGATGATGATGATGAAGGGAAAGCGGAGAGACTCAGATCCTTCTTGGTGTGGAAGAAGGAGGTGGTCGGTGGAGGAACAAGGGAGGAAGCCATTGATTGATGTCTGAGAAACAACAACGACAAAGTACACTGCAACAAGGTGTGGCGGCTGATGAAGAGACGTNGTTTTCTGGTTTTGTTTGATTATTTATTTTTACTGT
TARGET=167,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3892
PRIMER_LEFT_PENALTY=2.185841
PRIMER_RIGHT_PENALTY=0.203343
PRIMER_LEFT_SEQUENCE=TGGCTAGGGAATACAAAACTAACC
PRIMER_RIGHT_SEQUENCE=TCCTAGTTGTGGTGCTTGTTTG
PRIMER_LEFT=137,24
PRIMER_RIGHT=400,22
PRIMER_LEFT_TM=59.814
PRIMER_RIGHT_TM=60.203
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=2.4865
PRIMER_LEFT_1_PENALTY=2.283173
PRIMER_RIGHT_1_PENALTY=0.203343
PRIMER_LEFT_1_SEQUENCE=CAGTTCTAAGTTTGGCTAGGGA
PRIMER_RIGHT_1_SEQUENCE=TCCTAGTTGTGGTGCTTGTTTG
PRIMER_LEFT_1=125,22
PRIMER_RIGHT_1=400,22
PRIMER_LEFT_1_TM=57.717
PRIMER_RIGHT_1_TM=60.203
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=2.5073
PRIMER_LEFT_2_PENALTY=2.185841
PRIMER_RIGHT_2_PENALTY=0.321492
PRIMER_LEFT_2_SEQUENCE=GGCTAGGGAATACAAAACTAACCA
PRIMER_RIGHT_2_SEQUENCE=ATAGAAACTTTCCAGGGCGAAT
PRIMER_LEFT_2=138,24
PRIMER_RIGHT_2=317,22
PRIMER_LEFT_2_TM=59.814
PRIMER_RIGHT_2_TM=60.321
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=2.5073
PRIMER_LEFT_3_PENALTY=2.185841
PRIMER_RIGHT_3_PENALTY=0.321492
PRIMER_LEFT_3_SEQUENCE=TGGCTAGGGAATACAAAACTAACC
PRIMER_RIGHT_3_SEQUENCE=ATAGAAACTTTCCAGGGCGAAT
PRIMER_LEFT_3=137,24
PRIMER_RIGHT_3=317,22
PRIMER_LEFT_3_TM=59.814
PRIMER_RIGHT_3_TM=60.321
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=2.6047
PRIMER_LEFT_4_PENALTY=2.283173
PRIMER_RIGHT_4_PENALTY=0.321492
PRIMER_LEFT_4_SEQUENCE=CAGTTCTAAGTTTGGCTAGGGA
PRIMER_RIGHT_4_SEQUENCE=ATAGAAACTTTCCAGGGCGAAT
PRIMER_LEFT_4=125,22
PRIMER_RIGHT_4=317,22
PRIMER_LEFT_4_TM=57.717
PRIMER_RIGHT_4_TM=60.321
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_03193 Ps_contig_mira-and-tgicl-ass_franssen_29083
SEQUENCE=GTAGATACTNNAAAAAGTACTAAACCTATTTTTTTAGTCACAAACTTAGAACAAGTTCATTAACAGTCACAAAATGTCTTCACAGAAGATAAATACCGATAGAGTTGTAAAACTTCTATAGCTTACAGTTCTAAGTTTGGCTAGGGAATACAAAACTAACCAATGCATACAATACAATACAAAAAGACAATACATCTTACTGCAAGAACTCTCTTGGATCAGTAACGTAACCGGTCAATGCAGATGCGGCAGCTGTATACGGGGAAGCCAAATATATTTCACCTTCCCTGTGGCCCATTCGCCCTGGAAAGTTTCTATTCGTAGTTGAAACACAAACCTGAGGTTCATTCATGCGAGCATAAGTATCTTTCGGGCCACCCAAACAAGCACCACAACTAGGACTAGCAGGTGTGTCGCAACCAGCTTCTTCAAATATCTGTGAGCAAGTCTTACCACCTGAACCGGGGACAGGGAGGCTGTACACATCCATCCAAACCTTCTGTGTTGCCGGGACAAGAAACGTCGGAACTTTAACCTTTTTACCCGATGCTAGAAAAACTTTTGCGGCAGCCATGAAATCTTCTGTTTTCCCACCCGTACAAGATCCTATGTATACTCTGTCAATTTTCACATCCTTGCACTCCCTTGCCAAAGCTCGGTTATCCGGTGAATGAGGCTTGGCCACCAGTGGCTCCAACTTTGTGACATCAAATCTGTACTCAGAAAGAAATCTTGCTTGCTGGTCACTATAAACTGGTTCATATGGCACAGATGTCTTGCCCTCAAGATATTTATATGTAATGCTGTCAGGAGGAATAACACCGTTCTTTCCTCCAGCTTCAACTACCATGTTGCACAATGTCATTCGTTCTTCCATAGTTAAACTTTCAACGGTTGAGCCAACAAACTCCATAGATTTGTAAGTTGCACCGGCCACAGATATTTCACCAATAATTTGCAGAATTAAATCCTTTGCAAGCAAATAACTTGGCATTTCTCCATCCAATACAAATCTCAGAGTTGGAGGCACCTTGAGCAAAAGCTTCCCTGCTCCCAACACAAAACCAGCATCGGTATTACCAATCCCTGTTGCAAATTGACCAAATGCTCCAGCAGTACAAGTGTGAGAATCAGTGCCCAAGAGAACCTCTCCTGGCCTACAATGACCTTCCTGAGCAAGAGCGATGTGACAAACACCTTTGTAATCTGGATTTACCTTAAAATTACTAAGATCCTTAATATCATAAAAGTACTTGATATTTTGCTCCTGACAAAAATCCCTGAGTATGTCTACATTGCGATTGGCACGTTCATCACTTGTGAATATATAGTGATCAGGTATTATTACAAGCTTTTCACGATCCCAAACCTTAGCATCCTCACCAAACTCTTTCTTAAAAATCCCAATAGAACCAGGACCACAGACATCATGTGTCATCAAAACATCAACATTGACCCAAATATTATCCCCAGGTATGGCCTTCTCCTTCTCAGCAGCTCTAGCCAATATCTTCTCAGTCATGGTCATGGCTGTCTTCACTGATCCAGTGGTAGAAGGCTGGCGCTGTGTTTGCTGAGGCGCGGTAACTGAACAAGAGATTCTCCTGGAAGCGTGTTTCTGACATCGCTGAGAAGATGATGATGATGATGATGAAGGGAAAGCGGAGAGACTCAGATCCTTCTTGGTGTGGAAGAAGGAGGTGGTCGGTGGAGGAACAAGGGAGGAAGCCATTGATTGATGTCTGAGAAACAACAACGACAAAGTACACTGCAACAAGGTGTGGCGGCTGATGAAGAGACGTNGTTTTCTGGTTTTGTTTGATTATTTATTTTTACTGT
TARGET=1635,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1225
PRIMER_LEFT_PENALTY=0.090770
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=TTGACCCAAATATTATCCCCAG
PRIMER_RIGHT_SEQUENCE=AGACATCAATCAATGGCTTCCT
PRIMER_LEFT=1451,22
PRIMER_RIGHT=1743,22
PRIMER_LEFT_TM=59.909
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.1717
PRIMER_LEFT_1_PENALTY=0.090770
PRIMER_RIGHT_1_PENALTY=0.080962
PRIMER_LEFT_1_SEQUENCE=TTGACCCAAATATTATCCCCAG
PRIMER_RIGHT_1_SEQUENCE=TCAGACATCAATCAATGGCTTC
PRIMER_LEFT_1=1451,22
PRIMER_RIGHT_1=1745,22
PRIMER_LEFT_1_TM=59.909
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=0.1795
PRIMER_LEFT_2_PENALTY=0.147734
PRIMER_RIGHT_2_PENALTY=0.031769
PRIMER_LEFT_2_SEQUENCE=GACCCAAATATTATCCCCAGGT
PRIMER_RIGHT_2_SEQUENCE=AGACATCAATCAATGGCTTCCT
PRIMER_LEFT_2=1453,22
PRIMER_RIGHT_2=1743,22
PRIMER_LEFT_2_TM=60.148
PRIMER_RIGHT_2_TM=59.968
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.2009
PRIMER_LEFT_3_PENALTY=0.090770
PRIMER_RIGHT_3_PENALTY=0.110131
PRIMER_LEFT_3_SEQUENCE=TTGACCCAAATATTATCCCCAG
PRIMER_RIGHT_3_SEQUENCE=TCTTCCACACCAAGAAGGATCT
PRIMER_LEFT_3=1451,22
PRIMER_RIGHT_3=1694,22
PRIMER_LEFT_3_TM=59.909
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.2258
PRIMER_LEFT_4_PENALTY=0.194065
PRIMER_RIGHT_4_PENALTY=0.031769
PRIMER_LEFT_4_SEQUENCE=ATATTATCCCCAGGTATGGCCT
PRIMER_RIGHT_4_SEQUENCE=AGACATCAATCAATGGCTTCCT
PRIMER_LEFT_4=1460,22
PRIMER_RIGHT_4=1743,22
PRIMER_LEFT_4_TM=59.806
PRIMER_RIGHT_4_TM=59.968
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_03194 Ps_contig_mira-and-tgicl-ass_franssen_29098
SEQUENCE=AATTTTAAATTAATAGNCCAATAAATTTAAATAGAAAAACTTGGTTTCTTGCTAGTGAGTTGCAAAGACTTGAGAGAAACAACTACAATTGATAATTTATTTCTATACATAACTTTAAATTACACAACTATTGCACAGAAGATCAAACTTATGCCCTTTACAACGATTATGACCTTTCAACATTGTAATGGCATCACTCATTCTAATCCAAAAACTGACTTGTACACTACAAGGAGAAAGAAAAGCAATGGGATGAAGAAAAAATGAAGTGAACAGAACAAACATATTTCAGACTCAATAAGTTACTAGTTGCTACCTCCATACCACATGCTGAGCAAACATTGACCTCACATGACAATTTCGGGTTTCAGAATATTTGTCTTAATCTCCTTGTGGGGCAAAACAACACCTCCATTGCTGTAAATCTCATCACAAACATGAACATCTTCTCCAAGGATAGTCATATTCTCCACTCGAGCCCATTGACCAACGGTGGAATGCCACCCAATGATACTGCTGGAAATGCAAGCATGTTTCTTAATCCGGACTCCTCGCATTACTGTGCAGCGTGAGAGCCTCACACCAGACTCAACAATGCAGCCGGGACCGATGGCGACATCAGGTCCAATGAGACATCCCTCGCCAATTTTGGCTGTTTCATCAACAATGACATTCCCCACAATGTTAGAGCCAATGGCCAGCTTAGAAGAAGACTTCTTCCTCAATGAGTCCAGGTAAAGCCTCAAGCCAGTAATATAGTCCCTCGGTTGTCCGATGTCCATCCAGAATCCAGGAAGGACCATTGCATATAGCTTTTTCTCTGCAGCAATCTTTGGAAAAATTTCTTTTTCAATAGAGGTGGGCCTCAGTTCAATTCGATCCAAAACAGATGGGTTCAATAGGTAAATTCCAGCATTGATTTTGTTACCAACAAACAACTTAGGTTTTTCAACAAATTTCTCAACCTGTCCCGTGGTCTCCTCTGTTACAACAACACCATATTTTGATGGCTCGTCAACCTTTGTAACCATTATGGAAGCCTCCCCTCCATGGGATTTGTGGAATTCAATCATTTCTTTAAGTGGGTACTCACTGATAACATCACTGTTGAGAACAAAAAATGGCTCTCCAGATTCATCTATCAGCTTATCCCTAGCAAGAGCCAGGGGACCTGCAGTTCCTAGTGGTTCAGTTTCTTGTGAACAACTAATCTTAATGCCTAGCTTTGCTTCAAAATCCTTCAAGAAATTCAACATAACCTCTGGTTGGTAATTGATGGCCAGTACCACTTCAGTAACTCCAGTGGCCTTAAGAGCCTCTATCTGATGCAAAATCATGGGTTTGTTAGCAAAATCAACAAGAGGCTTAGGGACACTGAGTGTCAATGGCCTCAACCTAGTTCCAAACCCTCCAACCAGAATCAATGCCTTCATTGTGATAAGTCCTTTGTTTTCCTCGGTTGTGGAGGAGTTAGATCCGCGTGAGAATTGAAAATCAAGATTCAGAAATGAGTCGTAGTAAAACCCCGACCCGGACCGAGTCAACACGGCGAGTCGGAATCTAGAACTAGAAGAAGAAGAAGAAGAATTAGATTTCGAAGAAAATCCCTCCCCCG
TARGET=1569,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0869
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.082508
PRIMER_LEFT_SEQUENCE=GTCAATGGCCTCAACCTAGTTC
PRIMER_RIGHT_SEQUENCE=GGAGGGATTTTCTTCGAAATCT
PRIMER_LEFT=1380,22
PRIMER_RIGHT=1610,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.917
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.2129
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.082508
PRIMER_LEFT_1_SEQUENCE=ATCAACAAGAGGCTTAGGGACA
PRIMER_RIGHT_1_SEQUENCE=GGAGGGATTTTCTTCGAAATCT
PRIMER_LEFT_1=1352,22
PRIMER_RIGHT_1=1610,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.917
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.2720
PRIMER_LEFT_2_PENALTY=0.189529
PRIMER_RIGHT_2_PENALTY=0.082508
PRIMER_LEFT_2_SEQUENCE=AACAAGAGGCTTAGGGACACTG
PRIMER_RIGHT_2_SEQUENCE=GGAGGGATTTTCTTCGAAATCT
PRIMER_LEFT_2=1355,22
PRIMER_RIGHT_2=1610,22
PRIMER_LEFT_2_TM=59.810
PRIMER_RIGHT_2_TM=59.917
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.2720
PRIMER_LEFT_3_PENALTY=0.189529
PRIMER_RIGHT_3_PENALTY=0.082508
PRIMER_LEFT_3_SEQUENCE=CAACAAGAGGCTTAGGGACACT
PRIMER_RIGHT_3_SEQUENCE=GGAGGGATTTTCTTCGAAATCT
PRIMER_LEFT_3=1354,22
PRIMER_RIGHT_3=1610,22
PRIMER_LEFT_3_TM=59.810
PRIMER_RIGHT_3_TM=59.917
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.3844
PRIMER_LEFT_4_PENALTY=0.301926
PRIMER_RIGHT_4_PENALTY=0.082508
PRIMER_LEFT_4_SEQUENCE=ACAAGAGGCTTAGGGACACTGA
PRIMER_RIGHT_4_SEQUENCE=GGAGGGATTTTCTTCGAAATCT
PRIMER_LEFT_4=1356,22
PRIMER_RIGHT_4=1610,22
PRIMER_LEFT_4_TM=60.302
PRIMER_RIGHT_4_TM=59.917
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_03195 Ps_contig_mira-and-tgicl-ass_franssen_29106
SEQUENCE=TCGGGACCGTTGTCCCTCNCATTCTTCATTTCTTCATTCTCTCTCTGCAATGAACCCTCCGATCGGGAAACAAACAACATCTTCTCCTATATCTTCTCCACCACCATCGCTCCAATCAAATCTTCAACAACAACAACTAACTCATGGCAAATGCAGCATCAGGTATGGCAGTCCATGATGACTGCAAGTTAAGGTTTATGGAGCTCAAGGCAAAAAGGACGTATAGGTTCATCGTTTATAAGATTGAGGAGAAGCAGAAGCAAGTCATTGTGGAGAAGCTTGGTGAACCAGTCCAAGGCTATGAAGATTTCACTGCATCCCTTCCTGCTGATGAGTGCCGTTATGCTGTTTATGATTTTGAGTATCTGACAGAAGGGAATGTCCCCAAGAGCAGAATTTTTTTCATTGGCTGGTCACCTGATACTGCAAGGGTGAGAAGCAAGATGATTTATGCAAGCTCCAAAGACAGGTTCAGGAGGGAGCTGGATGGGATTCATGTAGAGTTGCAGGCAACTGATCCTACTGAAATGGGTCTTGATGTGTTCAAAAGCCGTGTCAATTAAATAATGATTATGAATAGTATAGGCTTTTCTGGTGGGGGAGCTCTCCCCTTAAGCCTTAGTTGCTCATATTTGAGATATCGATAGTTTGTGGGTTGGTCAATTTGACTAGGTTATGGTCTCAAACTCTCAATTTTCCAAGTTGTGGCATAAAGTGTATTGCACCTTTGACAGTCTTCTCTTGTTCCTGTGTATTTTGTTATGATTTGTGATATATACAATCTTTGTGTTTGAGTGGCATTTTAGTCCCCCTATCCTTATAGGTGGAATTTGTAACTGTTTTGTGTGTTGAGACAACAAATGGGCTTCATTTTTTTTTTTAATATTGANCTAACTTAAGGT
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2456
PRIMER_LEFT_PENALTY=0.228464
PRIMER_RIGHT_PENALTY=0.017127
PRIMER_LEFT_SEQUENCE=GATCGGGAAACAAACAACATCT
PRIMER_RIGHT_SEQUENCE=CCTATACGTCCTTTTTGCCTTG
PRIMER_LEFT=60,22
PRIMER_RIGHT=226,22
PRIMER_LEFT_TM=60.228
PRIMER_RIGHT_TM=60.017
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.2540
PRIMER_LEFT_1_PENALTY=0.228464
PRIMER_RIGHT_1_PENALTY=0.025566
PRIMER_LEFT_1_SEQUENCE=GATCGGGAAACAAACAACATCT
PRIMER_RIGHT_1_SEQUENCE=AATGACTTGCTTCTGCTTCTCC
PRIMER_LEFT_1=60,22
PRIMER_RIGHT_1=268,22
PRIMER_LEFT_1_TM=60.228
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.2683
PRIMER_LEFT_2_PENALTY=0.251135
PRIMER_RIGHT_2_PENALTY=0.017127
PRIMER_LEFT_2_SEQUENCE=GTTGTCCCTCNCATTCTTCATT
PRIMER_RIGHT_2_SEQUENCE=CCTATACGTCCTTTTTGCCTTG
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=226,22
PRIMER_LEFT_2_TM=59.749
PRIMER_RIGHT_2_TM=60.017
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.2767
PRIMER_LEFT_3_PENALTY=0.251135
PRIMER_RIGHT_3_PENALTY=0.025566
PRIMER_LEFT_3_SEQUENCE=GTTGTCCCTCNCATTCTTCATT
PRIMER_RIGHT_3_SEQUENCE=AATGACTTGCTTCTGCTTCTCC
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=268,22
PRIMER_LEFT_3_TM=59.749
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.2878
PRIMER_LEFT_4_PENALTY=0.228464
PRIMER_RIGHT_4_PENALTY=0.059316
PRIMER_LEFT_4_SEQUENCE=GATCGGGAAACAAACAACATCT
PRIMER_RIGHT_4_SEQUENCE=CACAATGACTTGCTTCTGCTTC
PRIMER_LEFT_4=60,22
PRIMER_RIGHT_4=271,22
PRIMER_LEFT_4_TM=60.228
PRIMER_RIGHT_4_TM=60.059
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_03196 Ps_contig_mira-and-tgicl-ass_franssen_29107
SEQUENCE=CCTTTGTCCCTCACATTTCTCATTTCTCATTTCTCTCTCTGCAATCGAACCCTCGATCGAGAAAACAAACACATCTTCTCCTATATCTTCTCCACCACCATCGCTCCAATCAAATCTTCAACAACAACAACTAACTCATGGCAAATGCAGCATCAGGTATGGCAGTCCATGATGACTGCAAGTTAAGGTTTATGGAGCTCAAGGCAAAAAGGACGTATAGGTTCATCGTTTATAAGATTGAGGAGAAGCAGAAGCAAGTCATTGTGGAGAAGCTTGGTGAACCAGTCCAAGGCTATGAAGATTTCACTGCATCCCTTCCTGCTGATGAGTGCCGTTATGCTTGTTTATGATTTTGAGTATCTGACAGAAGGAATCGTCCCCAAGAGCAGTNAATTTTTTCATTGGCTGGTCACCTGATACTGCGAAGGGTGAGAAGCAAGATGTATTTATGCAAGCTACCAAAAGACGTAGGTTCGAGGGAGGGNGCTG
TARGET=119,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4025
PRIMER_LEFT_PENALTY=0.385416
PRIMER_RIGHT_PENALTY=0.017127
PRIMER_LEFT_SEQUENCE=CGATCGAGAAAACAAACACATC
PRIMER_RIGHT_SEQUENCE=CCTATACGTCCTTTTTGCCTTG
PRIMER_LEFT=53,22
PRIMER_RIGHT=220,22
PRIMER_LEFT_TM=59.615
PRIMER_RIGHT_TM=60.017
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.4123
PRIMER_LEFT_1_PENALTY=0.385416
PRIMER_RIGHT_1_PENALTY=0.026872
PRIMER_LEFT_1_SEQUENCE=CGATCGAGAAAACAAACACATC
PRIMER_RIGHT_1_SEQUENCE=TCATAAACAAGCATAACGGCAC
PRIMER_LEFT_1=53,22
PRIMER_RIGHT_1=349,22
PRIMER_LEFT_1_TM=59.615
PRIMER_RIGHT_1_TM=60.027
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.4253
PRIMER_LEFT_2_PENALTY=0.408180
PRIMER_RIGHT_2_PENALTY=0.017127
PRIMER_LEFT_2_SEQUENCE=CATTTCTCTCTCTGCAATCGAA
PRIMER_RIGHT_2_SEQUENCE=CCTATACGTCCTTTTTGCCTTG
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=220,22
PRIMER_LEFT_2_TM=59.592
PRIMER_RIGHT_2_TM=60.017
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.4337
PRIMER_LEFT_3_PENALTY=0.408180
PRIMER_RIGHT_3_PENALTY=0.025566
PRIMER_LEFT_3_SEQUENCE=CATTTCTCTCTCTGCAATCGAA
PRIMER_RIGHT_3_SEQUENCE=AATGACTTGCTTCTGCTTCTCC
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=59.592
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.4447
PRIMER_LEFT_4_PENALTY=0.385416
PRIMER_RIGHT_4_PENALTY=0.059316
PRIMER_LEFT_4_SEQUENCE=CGATCGAGAAAACAAACACATC
PRIMER_RIGHT_4_SEQUENCE=CACAATGACTTGCTTCTGCTTC
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=265,22
PRIMER_LEFT_4_TM=59.615
PRIMER_RIGHT_4_TM=60.059
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_03197 Ps_contig_mira-and-tgicl-ass_franssen_29115
SEQUENCE=TTAGTTAGTTAGTTACACTAGTTTGTTATTCTCGTTTCATTTATATAATTTTTTTTCTAATATTTTTTCTATAACTTTTGCAGGTGAAGCAATTGGGGCTGAAGCAACTTTGCCTTACTTAAGTCCTCAACTTAACGGGCAAAGATTGCTTGTTGGTGCGAATTTTGCTTCGGCTGGCATTGGAATTCTCAATGACACTGGAATCCAATTTGTAAACATAATTAGAATCTTTAGACAATTGCAGTATTTTCAAGAATATCAACAGAGAGTGAGTGGTCTTATAGGACCTGCAAGAACGCAGAGATTAGTGAATGGAGCATTAGTACTAATCACTCTGGGAGGAAATGATTTTGTGAATAATTATTATTTGGTGCCTTATTCTGCAAGATCGCGCCAATACTCTTTACCGGATTATGTCAGATATATCATATCTGAATACAAGAAGATTCTAAGGAGGTTACATGATCTTGGAGCACGAAGGGTGATAGTGACAGGAACTGGTCCAATTGGTTGTGTTCCAGCAGAATTAGCTCAAAGAAGCACAAATGGAGAATGCTCACCTGAGTTACAACGTGCAGCAGGATTGTTCAATCCGCAACTTATTGAAATAATCCAACAACTCAACAATGAAATCGGTGAAAATGTTTTCATAGGAGCCAACACCAGACAAATGGCTCTCAACTTCATCACTAGCCCGGGAGCATACGGATTTGTTACATCAAAGGTAGCATGTTGTGGACAAGGACCCTACAATGGGCTTGGCCTATGCACACCACTCTCAAATTTATGCCCAAATCGTGACATTAATGCATTTTGGGATGCTTTCCATCCATCAGAAAAGGCTAACAGAATTATTGTTAATCAGATCATGTCAGGCACAACAGAATATATGTATCCAATGAATCTCAGCACTGCTTTAGCATTGGATTCCAACAAAAATATATAGTTAATTAATTTGATTTTCTTAGTATTGCGGCAACTTTTTTATATGTTAAAAGAAGATTTACTTGTTAATTTGTGATTTTTATGTATAAGTTGAATTTGAGTTGATGTACTAGTGGGGGATGGATGAGGATGAGTACTGTCTTTATTGTCAATCTCTATTGGCAATATAAAAGCCACTGCATATGTAATAATTGAGTTGTTTTTTTTGTCTCCAATCATTTTCTTTTTCATTGCCCATTTCTTCTTTTTAATTTTAAATTTAGATTAAGTTAATTAAA
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03198 Ps_contig_mira-and-tgicl-ass_franssen_29132
SEQUENCE=GTNGTAAATTAGCAGCTTGACTTAAACAATAAACGAAAATAACAAAATAGTCACATTCTTGAATCAAGACTATAGTATAAGAATTCTACACAATACAAACATTCTTAACCACTTTATTAGATTATTAAACATTTAACAATCGATGCTCTATCTTATCAAATTTCTCAATTCTGATAAAATGGAAATACAATTATGAATCAACAACAATCACTACAAACTACACATGAATACGCTTTAAGACAGCATTGGCAAGAATACAGATAATTATGCACTTGCAGTGATGCACTGGATTGCCTGTTTGCATATACCAGTCATTGTAGCTTCAGGTCCATAAACCTCAATTGGAATGCCAATTTCCTCTCCAAGTTCCCTCATTTTTGCTAGACCCCTCTGGTAGTTGGGACCTCCTCTCCTCACATAAATGTGCATATTTGCTTCTTTTAGCTTTTGTTCCTTCTCCTTCAGAGCTCGAATGATGCCACTGAATGTAGCAGCAACATCAGTGAAGTTAGCAATTCCTCCTCCGATCACAAGGGCTCTCTTTTGGCCATCAGGATTTGCAGTTGCACAATCGATTACAACTCTGGCATACTGCAAGACCTCATCTTCCTTTGGTGCGCCACTGTATTCTGCATAGTTTCCGAGCTCTGGAGCATAACCAAGATCTCCTACCGTATCAGCGTAGATCACACTGGCACCTCCTCCAGCTACCATAGTCCAAATTCGACCCAACGGGTTTAACACTGTAAATTTCAATGATGCACTTGTCTTTTCATCTAATCCATGAATGAAAGACTCGGTGGGACTCATAACCCTTCCAAATGGCAGAGGGAATTCAATGTCTCCCCATTTCTTGAAGTTCTTGAAAGCAGCAGTGTCATCTAGCTCTCCTCTCATATCCAAAGGATAAGGCTTTCCATCCACCAGGGCGAAAGGATTCATCTCTAAGAATGTGAAATCCAGATCTTGAAATATATTGAAAACCACCTTGAGAAAATCCTCAAGTTCTCCCTTGATCTCCAAGGGGAGGGTTGCAATAAGTGCTGATATATTCTCTGATGTAAGAGATTCCCCTGTTGGAATAAATACAGTCTTCACCTTATCCCAGTTCTCTTCAATGTCAATTCCTCCACATTCAGAAAAGCTTATACTGTTTCCCAGTCTCTCAGAGACAATGTTAAGGTAAAACTCTTCATCATGTGGAATGAAAGGTTCAACAATGAAAGTTGTTATAGGTCCTTTGCATCCACCCATCTCAACCTCTTTTCCAAGACGCTCTTTTACAAAACCAGCAACTTCAGCCAAATCCAGATTTAAAGCAACCAAGCCACTTTTGCCACGCTTTCCAAACAACATGTCAGGTTTCACAACCAATTTTGAAGATGAAAGCCATGGTTCCTTCTCTACTAGCTCATTGATATCAGTTGTTTCTGTAACTTGTGCAGACTTAATTGGCAAATCCTTTCCAGAAATTTTCTTAAAATGCTCCTTCAACAACCTCTTAGAATCATACTCTCTGATCTTCTTGCGTGCCATAGATCTTTCTTAATTTTCCAACACAAATTATTTCCTTCAAACCGTTCGAGAGAGAGAAACGGATGAATGGAGAAGAAGGTGAGAGATAGGTAGATCCAATGGCGAGTTTGGTGACTCTGTGAAATGAGAATTTGCATTTATAGAGATTACGGAAAAGAAGAAATTAAGATCCCCGAC
TARGET=1584,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1381
PRIMER_LEFT_PENALTY=0.124655
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=TCCAAACAACATGTCAGGTTTC
PRIMER_RIGHT_SEQUENCE=AACTCGCCATTGGATCTACCTA
PRIMER_LEFT=1344,22
PRIMER_RIGHT=1643,22
PRIMER_LEFT_TM=59.875
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.1388
PRIMER_LEFT_1_PENALTY=0.125411
PRIMER_RIGHT_1_PENALTY=0.013401
PRIMER_LEFT_1_SEQUENCE=TGCGTGCCATAGATCTTTCTTA
PRIMER_RIGHT_1_SEQUENCE=AACTCGCCATTGGATCTACCTA
PRIMER_LEFT_1=1526,22
PRIMER_RIGHT_1=1643,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=0.1732
PRIMER_LEFT_2_PENALTY=0.124655
PRIMER_RIGHT_2_PENALTY=0.048570
PRIMER_LEFT_2_SEQUENCE=TCCAAACAACATGTCAGGTTTC
PRIMER_RIGHT_2_SEQUENCE=TCGCCATTGGATCTACCTATCT
PRIMER_LEFT_2=1344,22
PRIMER_RIGHT_2=1640,22
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=59.951
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=0.1732
PRIMER_LEFT_3_PENALTY=0.124655
PRIMER_RIGHT_3_PENALTY=0.048570
PRIMER_LEFT_3_SEQUENCE=TCCAAACAACATGTCAGGTTTC
PRIMER_RIGHT_3_SEQUENCE=CTCGCCATTGGATCTACCTATC
PRIMER_LEFT_3=1344,22
PRIMER_RIGHT_3=1641,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=59.951
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.1732
PRIMER_LEFT_4_PENALTY=0.124655
PRIMER_RIGHT_4_PENALTY=0.048570
PRIMER_LEFT_4_SEQUENCE=TCCAAACAACATGTCAGGTTTC
PRIMER_RIGHT_4_SEQUENCE=CGCCATTGGATCTACCTATCTC
PRIMER_LEFT_4=1344,22
PRIMER_RIGHT_4=1639,22
PRIMER_LEFT_4_TM=59.875
PRIMER_RIGHT_4_TM=59.951
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_03199 Ps_contig_mira-and-tgicl-ass_franssen_29147
SEQUENCE=TCGGGGGACACTCTTTTCTCTTCACTCTCACTACAATGGAAGAGAACTACAACAAAAGAACCCTTCTCTTCCTATTATTAGCTATCTCCTTCACACTCTCATCCTCCACCACCACCACTCTTTTCCAAACCCAAACTCTACCTATTCACTCTCTCCCTAACCCTTCAACCCTCTCATGGCCACAAGAATCAGAACAAGAATCTCTATCCGACTCTGAACCTCTCTCACTTCAACTACACCACATAGACTCACTCTCCTCCAACAAAACACCACAACAACTCTTCCACCTCAGACTCCAAAGAGACGCTGCCAGAGTCCAATCCTTCAACCTCGCAGTATCTCCTAACCACTCCCGTCCCCTTAGATCCGGTACCGGTTTCAGCAGCTCCATCGTTTCTGGTCTTTCACAAGGCAGCGGCGAGTATTTCACACGCATAGGTGTCGGCACTCCAGCCAAGTACGTTTTCATGGTTCTCGACACCGGAAGCGACGTCGTTTGGCTTCAATGTGCTCCATGCCGTAAATGCTACTCACAAGCAGATCCAGTTTTCGATCCGACAAAGTCACGCACCTTCGCCGGGATCCCATGCGGTGCTCCTCTCTGCCGGAGACTCGACTCTGCCGGATGCAACAACAAGAATAAGGTTTGTCAGTATCAGGTTTCTTACGGTAATGGTTCTTTCACTTTCGGTGATTTCTCCACTGAAACGCTGACGTTTCGAAAGACGCGTGTGACGCGCGTGGCTTTGGGATGTGGTCATGATAATGAAGGTCTCTTCGTTGGTGCTGCTGGTTTATTGGGACTTGGACGTGGAAGATTATCTTTCCCGGTTCAAACCGGACGCCGGTTTAATCAGAAATTCTCTTATTGTCTTGTGGACCGGTCCGCTTCAGCTAAACCATCTTCCATGGTTTTTGGCGATTCAGCTATTTCACGAATCGCCCGGTTTACTCCACTCCTCAAAAACCCTAAACTCGATACTTTCTATTACATTGAACTTCTCGGAATCAGTGTCGGTGGTGCATCCGTTCGCGGCGTCTCCGCTTCTCTCTTCAAGCTCGATCCCGCCGGTAACGGCGGAGTTATCATTGATTCAGGCACGTCAGTAACCCGATTGACACGTCCCGCTTACATTGCACTGAGAGATGCTTTTAGGCTTGGTGCTTCTCATCTGAAGAAGGCACCGGAGTTCTCGCTGTTCGATACTTGCTTTGATTTGTCAGGGCTGACGGAGGTGAAGGTGCCGACGGTGGTGCTTCATTTCCGAGGTGCTGACGTGTCACTTCCAGCTACGAATTACTTGATTCCGGTGGATAACAGTGGAAGCTTCTGCTTTGCTTTCGCCGGAACAATGAGTGGGTTGTCCATCATCGGTAACATTCAGCAGCAAGGATTTCGGGTCGTTTATGATCTCGCGGGTTCTCGGGTCGGGTTTGCCCCGAGAGGGTGCGTCTGAGTGAGAAATCATGGGAATAACTCTTTCCCTTTCTTTTGTTTTTCGATTATTATCTTTTTTTTAATTGTCTTTTTTTAATCAAGTTTATTAAAGTTACTGTATTATTATAGTTTAGAGAATGGATTAGGGAATATGAAATGTTAATAATCTTTTTAGATCTGAAGAGGATCTAAAGGATTAAACAAAATGAGAATGTTTTTTTAGTTTCCACCGCCAC
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7784
PRIMER_LEFT_PENALTY=0.769510
PRIMER_RIGHT_PENALTY=0.008930
PRIMER_LEFT_SEQUENCE=GGGGACACTCTTTTCTCTTCAC
PRIMER_RIGHT_SEQUENCE=CATGAGAGGGTTGAAGGGTTAG
PRIMER_LEFT=3,22
PRIMER_RIGHT=177,22
PRIMER_LEFT_TM=59.230
PRIMER_RIGHT_TM=59.991
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=175
PRIMER_PAIR_PENALTY_1=0.9341
PRIMER_LEFT_1_PENALTY=0.769510
PRIMER_RIGHT_1_PENALTY=0.164595
PRIMER_LEFT_1_SEQUENCE=GGGGACACTCTTTTCTCTTCAC
PRIMER_RIGHT_1_SEQUENCE=AGTTGTTGTGGTGTTTTGTTGG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=279,22
PRIMER_LEFT_1_TM=59.230
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=277
PRIMER_PAIR_PENALTY_2=0.9665
PRIMER_LEFT_2_PENALTY=0.769510
PRIMER_RIGHT_2_PENALTY=0.197024
PRIMER_LEFT_2_SEQUENCE=GGGGACACTCTTTTCTCTTCAC
PRIMER_RIGHT_2_SEQUENCE=CTGAGGTGGAAGAGTTGTTGTG
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=291,22
PRIMER_LEFT_2_TM=59.230
PRIMER_RIGHT_2_TM=59.803
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=1.1621
PRIMER_LEFT_3_PENALTY=0.769510
PRIMER_RIGHT_3_PENALTY=0.392589
PRIMER_LEFT_3_SEQUENCE=GGGGACACTCTTTTCTCTTCAC
PRIMER_RIGHT_3_SEQUENCE=TGAGAGAGGTTCAGAGTCGGAT
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=226,22
PRIMER_LEFT_3_TM=59.230
PRIMER_RIGHT_3_TM=60.393
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=1.1659
PRIMER_LEFT_4_PENALTY=1.156983
PRIMER_RIGHT_4_PENALTY=0.008930
PRIMER_LEFT_4_SEQUENCE=GGGGACACTCTTTTCTCTTCACT
PRIMER_RIGHT_4_SEQUENCE=CATGAGAGGGTTGAAGGGTTAG
PRIMER_LEFT_4=3,23
PRIMER_RIGHT_4=177,22
PRIMER_LEFT_4_TM=60.157
PRIMER_RIGHT_4_TM=59.991
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_03200 Ps_contig_mira-and-tgicl-ass_franssen_29158
SEQUENCE=ATCTTAACCTTAAGTTCTTGAGTTCATCTTCTTTTGAGTTTGGTAACAGTATTAGAATGTCACCAATAGTTACCAAACTCAAGAACTCAAGAACTTAAGGTTAAGATCCCAACAAACCAAAAAGGCCTCCCAGCGCTTTCTTCGTCTTCCTTGAGGATTTCAGAAAGACTTTTAAGGCAGAAAATCCTAATGTGAAGGCTGTATCGGCTGTTGGGAAGGCTGGAGGAGAGAAATGGAAATCCTCGACTAAGGCTGAAAAGCTCCATATGAAGCCAAGGCTGACAAAAAGGAAGTTGAATATAGAAAAACTTNTGAACGTCTTTAAATAAATAAACGAAAGGCCGGACTAA
TARGET=323,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03201 Ps_contig_mira-and-tgicl-ass_franssen_29171
SEQUENCE=AACTTGCTAAATGATTCTATAACTTGCAGCATAAAATAGACCAATTGCAAACCACATGTAAAAGTTAAAGACCATTGCATCTTTACTAGTTAAGCAACAGAAACAAGATGCATCCAGTAGTTATTATTAACATATGCTACTCCATGGATTAAAGTTCCATAGAACCGAAACCCGAAATTACAACGAAACAGACAAAGATGGCTCTCTGACCAAAACATATTTACAAACACTCGAGCTTATTACATGATTCGAGTCAGCTTGAGTGTTGTTCCAGGGATTTTTTCCGTCATCTAACAATAGACTAATAACCCCCTTGTATGTAAGCATTCAAGCGGTCAAGCTCTTGACGATGCTCGCCCACGACAGCACGAACGACGTCTCCGATGGACACCATGCCGAGCATTTCTTTGTCGCTTATCACAGGGATGTGTCTGATCCTGTTATCTGTCATCAGCTGCATTGCTCGAAGAACTTTGGTATCAGGGGTAACTGTGATAAGTTTGTTCTCTTCAGTCATAATATCGCCAACCTTGGTGGACTTGGATGACCTTCCCTGCACGATGATCTTCCTTAGGTAATCTCTTTCTGTTATAATTCCAGCAATTGATTTCTCCTCGCCTGGTTTCACGACCACCAAAGCTCCGACGTTGTTTTGCGTCATCGATTTCACAGCTTCATAGACAGTGTCGTCCGTGGTGCACCAAAGCCAGGAACCATCTGCGCCTTTACCTTTGCCTTGCAAGATGTCTGAAATTGTAGTGCTCTCAAAACCATGCTCCTCAATACGGGCAGGTGTAACGGACTCAAACCGCGAAAAGGCAGCTGGCTGCAACAGAGGATTCACCATGCGGACTCGTTGCAAAACAGCATTTTTTATGACGTTCCCGTTCGACAAAAATGATCGCAATGCTCCTTGCATTTTAGCTGTCAGAGAGTGAGTGAGTGAGAGAAGGTTTCGGGTAGATGGTTGAGGTGAGGAGGCGTATGGACAAGACAGGAACACTTGACTTGTTCCTCAAATTTCACCNNAAAAAAATGTGAGAGGTGGTGAGTGGGCCTTTTCTAGAGTTTTGGATCATGACATTATCCAAACTATCATTATTATATTTTGTTTATTATTGCAACTTGTAAGTAGGTAATTCTATTGATAATGAAAGACTTAAA
TARGET=933,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2591
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=CCTTGCAAGATGTCTGAAATTG
PRIMER_RIGHT_SEQUENCE=CCTCCTCACCTCAACCATCTAC
PRIMER_LEFT=734,22
PRIMER_RIGHT=980,22
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=247
PRIMER_PAIR_PENALTY_1=0.2591
PRIMER_LEFT_1_PENALTY=0.253482
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=CCTTGCAAGATGTCTGAAATTG
PRIMER_RIGHT_1_SEQUENCE=CTCCTCACCTCAACCATCTACC
PRIMER_LEFT_1=734,22
PRIMER_RIGHT_1=979,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=0.3185
PRIMER_LEFT_2_PENALTY=0.312895
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=TGTAGTGCTCTCAAAACCATGC
PRIMER_RIGHT_2_SEQUENCE=CTCCTCACCTCAACCATCTACC
PRIMER_LEFT_2=754,22
PRIMER_RIGHT_2=979,22
PRIMER_LEFT_2_TM=60.313
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.3185
PRIMER_LEFT_3_PENALTY=0.312895
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=TGTAGTGCTCTCAAAACCATGC
PRIMER_RIGHT_3_SEQUENCE=CCTCCTCACCTCAACCATCTAC
PRIMER_LEFT_3=754,22
PRIMER_RIGHT_3=980,22
PRIMER_LEFT_3_TM=60.313
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.4838
PRIMER_LEFT_4_PENALTY=0.478252
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=TAGTGCTCTCAAAACCATGCTC
PRIMER_RIGHT_4_SEQUENCE=CTCCTCACCTCAACCATCTACC
PRIMER_LEFT_4=756,22
PRIMER_RIGHT_4=979,22
PRIMER_LEFT_4_TM=59.522
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_03202 Ps_contig_mira-and-tgicl-ass_franssen_29172
SEQUENCE=CCTCGTCCTTGTCCATACGCCTCCTCACCCTCAACCCAACTCTACCCGAAACCTTACTCTCACTCACTCACTCTCTGCTAAAATAGCAAGGAGCATTGCGATCATTTTTGTCGAACGGGAACGTCATAAAAAATAGCTGTTTTGCAACGAGTCCGCATGGTGAATCCTCTGTTGCAGCCAGCTGCCTTTTCGCGGTTTGAGTCCGTTACACCTGCCCGTATTGAGGAGCATGGTTTTGAGAGCACTACAATTTCAGACATCTTGCAAGGCAAAGGTAAAGGCGCAGATGGTTCCTGGCTTTGGTGCACCACGGACGACACTGTCTATGAAGCTGTGAAATCGATGACACAAAACAACGTCGGAGCTTTGGTGGTCGTGAAACCAGGCGAGGAGAAATCAATTGCTGGAATTATAACAGAAAGAGATTACCTTAGGAAGATCATCGTGCAGGGAAGGTCATCCAAGTCCACCAAGGTTGGTGATATTATGACTGAAGAGAACAAACTTATCACAGTTACCCCTGATACCAAAGTTCTTCGAGCAATGCAGCTGATGACAGATAACAGGATCAGACACATCCCTGTCATAAGCGACAAAGAAATGCTCGGCATGGTGTCCATCGGAGACGTCGTTCGTGCTGTCGTTGGCGAGCATCGTCAAGAGCTTGACCGCTTGAATGCTTACATACAGGGGGGTTATTAGTCTATTGTTAGATGACGGAAAAAATCCCTGGAACAACACTCAAGCTGACTCGAATCATGTAATAAGCTCGAGTGTTTGTAAATATGTTTTGGTCAGAGAGCCATCTTTGTCTGTTTCGTTGTAATTTCGGGTTTCGGTTCTATGGAACTTTAATCCATGGAGTAGCATATGTTAATAATAACTACTGGATGCATCTTGTTTCTGTTGAACC
TARGET=59,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2846
PRIMER_LEFT_PENALTY=0.031122
PRIMER_RIGHT_PENALTY=0.253482
PRIMER_LEFT_SEQUENCE=CTCACCCTCAACCCAACTCTAC
PRIMER_RIGHT_SEQUENCE=CCTTGCAAGATGTCTGAAATTG
PRIMER_LEFT=23,22
PRIMER_RIGHT=268,22
PRIMER_LEFT_TM=60.031
PRIMER_RIGHT_TM=59.747
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.3440
PRIMER_LEFT_1_PENALTY=0.031122
PRIMER_RIGHT_1_PENALTY=0.312895
PRIMER_LEFT_1_SEQUENCE=CTCACCCTCAACCCAACTCTAC
PRIMER_RIGHT_1_SEQUENCE=TGTAGTGCTCTCAAAACCATGC
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=60.031
PRIMER_RIGHT_1_TM=60.313
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.5094
PRIMER_LEFT_2_PENALTY=0.031122
PRIMER_RIGHT_2_PENALTY=0.478252
PRIMER_LEFT_2_SEQUENCE=CTCACCCTCAACCCAACTCTAC
PRIMER_RIGHT_2_SEQUENCE=TAGTGCTCTCAAAACCATGCTC
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=60.031
PRIMER_RIGHT_2_TM=59.522
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.8063
PRIMER_LEFT_3_PENALTY=0.031122
PRIMER_RIGHT_3_PENALTY=0.775205
PRIMER_LEFT_3_SEQUENCE=CTCACCCTCAACCCAACTCTAC
PRIMER_RIGHT_3_SEQUENCE=CTCAAAACCATGCTCCTCAATA
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=239,22
PRIMER_LEFT_3_TM=60.031
PRIMER_RIGHT_3_TM=59.225
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=0.9404
PRIMER_LEFT_4_PENALTY=0.031122
PRIMER_RIGHT_4_PENALTY=0.909234
PRIMER_LEFT_4_SEQUENCE=CTCACCCTCAACCCAACTCTAC
PRIMER_RIGHT_4_SEQUENCE=TCAAAACCATGCTCCTCAATAC
PRIMER_LEFT_4=23,22
PRIMER_RIGHT_4=238,22
PRIMER_LEFT_4_TM=60.031
PRIMER_RIGHT_4_TM=59.091
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_03203 Ps_contig_mira-and-tgicl-ass_franssen_29174
SEQUENCE=CAATAACTATAATAAGAGTATATATATAAATACCCAATTCCTTCTTTTTCTCTTCTTCTTACATTTTCCACATCTTCTTCTCCTCTTTCCCCTTTGCAGGAACCAAATCTTCTTCCAATGGCCGATCAGCTCACCGACGAACAGATCTCCGAGTTCAAGGAAGCTTTCAGTCTATTCGACAAGGATGGCGATGGTTGCATCACTACCAAGGAGCTCGGGACTGTTATGCGATCACTTGGCCAGAACCCAACTGAGGCCGAGTTGCAGGACATGATAAACGAGGTTGATGCTGATGGAAACGGTACCATTGATTTCCCTGAATTCCTCAACCTGATGGCCCGTAAGATGAAGGATACTGATTCCGAGGAAGAGCTTAAGGAAGCTTTCCGTGTGTTCGACAAGGATCAGAATGGTTTCATCTCTGCAGCTGAGCTTCGTCATGTTATGACAAATCTCGGTGAGAAGCTGACTGATGAAGAGGTCGACGAGATGATCCGCGAAGCTGATGTTGATGGTGATGGTCAGATCAACTACGAGGAGTTTGTCAAAGTCATGATGGCTAAGTGAAAGGATCAAACAAAACGATAACCAAAATTTGAAAAAGCATATGAACAGAAAAGGGACAAAGCTAATGAGTTTTGATGAGGATTATTATTACTATTCTATTTTTCATTAGGACATGTTTTGTTTGGGTTATTAGTTTTTTGTTGGTTGGTTTGTTTGCTTTAATTATTGCCACTTGTCATTATTAGTATTCTACTTGTTTGGTTTGTCCCTTTGTTTAAGTAGTTTGAATTAGTTGTGTTTTACCATGCATCTGTACTTCTATTTTTATTCTTTTTTTTGGATTATTATTCATGAACATGGAACAATTGTTTGCAATTTGTGACTTTTACATGTTCACTACATGATTGTTTCTAGGGGGTT
TARGET=19,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03204 Ps_contig_mira-and-tgicl-ass_franssen_29180
SEQUENCE=GGATTTTCATTTCATTAGCAATTGAATTTCAATTTCCCTTTAAGGCCTTTAATATCCACCCGCATTTGTCCTAGCCTTATGTCATAGTCTCATTCACAACAAACGCATATCACAAACACCTTCTCTCTCTCCCTCTCTCCCAAACCCTAGTATCTATCTCTCTTCACCGTTCGGTTTCATCATGAAAGGAGGAAAGTCCAAGGGTGAATCGAAGAAAGCTGAAACGAAGCTTGCGGTGAATAAGAAGGGTGCTCCCGCCACAAAGGGCGGAAAGAAACCAGCCAAGGGAAAGGAACCAAAGGACCCTAACAAGCCGAAGAGACCTCCAAGTGCTTTCTTCGTTTTCATGGAGGACTTTAGGAAGCAGTTCAAAAAGGAAAACCCTGACAACAAAGCTGTGTCTGCAGTGGGCAAAGCTGCTGGGGCAAAATGGAAGTCAATGACTGAAGCTGAGAAAGCACCATATGCTGCTAAGGCAGAGAAAAGGAAGGCTGAATATGAGAAGAGCATGAAGTCCTATAACAAGAAACAAGCTGAAGGTCCAGCTGCTGTTGAAGAAGAAGAAGAATCCGAGAAATCAGAATCTGAGGTGCATGATGAGAATGATGATGAGGAAGAAAGTGAGGAGGAGGATGATGACGAGTAGAAAATTGGATGATGATAACATTTATGTAGGATGCTATGCTGATGATTTGATCATCTTATGTAGATGATTGTCATTTTATGATAAAAATGTCTTGCTACCCAAATATTTTGCTGCTCTTGGCTAATGTTTTGTCTGTTCAAAACGTGAATAGTTAAAAATGAGGGAGCAAACTTTTATTTGTACTTTAAGGCCAACTCTTTAGTTAAGAGATGTTTATCTTTTGTAGAGCTTTTAGATTTTCATGATGGAATCTCTTGATGTTTGTATTAACATAATTTCCTTATTAGCTTTTCATATCCTTTGTGCTGTATCATTGTTTGGTGTTGTCCATTATCTGATGATTTTGTTAACTTCTGTCAATGGTTTAATTCTAACTATAAAATTCTTTTAGTTAAAAACCT
TARGET=122,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0501
PRIMER_LEFT_PENALTY=0.044457
PRIMER_RIGHT_PENALTY=0.005674
PRIMER_LEFT_SEQUENCE=GTCTCATTCACAACAAACGCAT
PRIMER_RIGHT_SEQUENCE=CCTAAAGTCCTCCATGAAAACG
PRIMER_LEFT=86,22
PRIMER_RIGHT=360,22
PRIMER_LEFT_TM=60.044
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.1000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.0527
PRIMER_LEFT_1_PENALTY=0.044457
PRIMER_RIGHT_1_PENALTY=0.008292
PRIMER_LEFT_1_SEQUENCE=GTCTCATTCACAACAAACGCAT
PRIMER_RIGHT_1_SEQUENCE=CAGGGTTTTCCTTTTTGAACTG
PRIMER_LEFT_1=86,22
PRIMER_RIGHT_1=385,22
PRIMER_LEFT_1_TM=60.044
PRIMER_RIGHT_1_TM=60.008
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.0657
PRIMER_LEFT_2_PENALTY=0.044457
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=GTCTCATTCACAACAAACGCAT
PRIMER_RIGHT_2_SEQUENCE=CTTCGTTTCAGCTTTCTTCGAT
PRIMER_LEFT_2=86,22
PRIMER_RIGHT_2=228,22
PRIMER_LEFT_2_TM=60.044
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.1000
PRIMER_RIGHT_2_END_STABILITY=8.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=143
PRIMER_PAIR_PENALTY_3=0.0796
PRIMER_LEFT_3_PENALTY=0.073900
PRIMER_RIGHT_3_PENALTY=0.005674
PRIMER_LEFT_3_SEQUENCE=CAAACGCATATCACAAACACCT
PRIMER_RIGHT_3_SEQUENCE=CCTAAAGTCCTCCATGAAAACG
PRIMER_LEFT_3=99,22
PRIMER_RIGHT_3=360,22
PRIMER_LEFT_3_TM=59.926
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.0822
PRIMER_LEFT_4_PENALTY=0.073900
PRIMER_RIGHT_4_PENALTY=0.008292
PRIMER_LEFT_4_SEQUENCE=CAAACGCATATCACAAACACCT
PRIMER_RIGHT_4_SEQUENCE=CAGGGTTTTCCTTTTTGAACTG
PRIMER_LEFT_4=99,22
PRIMER_RIGHT_4=385,22
PRIMER_LEFT_4_TM=59.926
PRIMER_RIGHT_4_TM=60.008
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_03205 Ps_contig_mira-and-tgicl-ass_franssen_29180
SEQUENCE=GGATTTTCATTTCATTAGCAATTGAATTTCAATTTCCCTTTAAGGCCTTTAATATCCACCCGCATTTGTCCTAGCCTTATGTCATAGTCTCATTCACAACAAACGCATATCACAAACACCTTCTCTCTCTCCCTCTCTCCCAAACCCTAGTATCTATCTCTCTTCACCGTTCGGTTTCATCATGAAAGGAGGAAAGTCCAAGGGTGAATCGAAGAAAGCTGAAACGAAGCTTGCGGTGAATAAGAAGGGTGCTCCCGCCACAAAGGGCGGAAAGAAACCAGCCAAGGGAAAGGAACCAAAGGACCCTAACAAGCCGAAGAGACCTCCAAGTGCTTTCTTCGTTTTCATGGAGGACTTTAGGAAGCAGTTCAAAAAGGAAAACCCTGACAACAAAGCTGTGTCTGCAGTGGGCAAAGCTGCTGGGGCAAAATGGAAGTCAATGACTGAAGCTGAGAAAGCACCATATGCTGCTAAGGCAGAGAAAAGGAAGGCTGAATATGAGAAGAGCATGAAGTCCTATAACAAGAAACAAGCTGAAGGTCCAGCTGCTGTTGAAGAAGAAGAAGAATCCGAGAAATCAGAATCTGAGGTGCATGATGAGAATGATGATGAGGAAGAAAGTGAGGAGGAGGATGATGACGAGTAGAAAATTGGATGATGATAACATTTATGTAGGATGCTATGCTGATGATTTGATCATCTTATGTAGATGATTGTCATTTTATGATAAAAATGTCTTGCTACCCAAATATTTTGCTGCTCTTGGCTAATGTTTTGTCTGTTCAAAACGTGAATAGTTAAAAATGAGGGAGCAAACTTTTATTTGTACTTTAAGGCCAACTCTTTAGTTAAGAGATGTTTATCTTTTGTAGAGCTTTTAGATTTTCATGATGGAATCTCTTGATGTTTGTATTAACATAATTTCCTTATTAGCTTTTCATATCCTTTGTGCTGTATCATTGTTTGGTGTTGTCCATTATCTGATGATTTTGTTAACTTCTGTCAATGGTTTAATTCTAACTATAAAATTCTTTTAGTTAAAAACCT
TARGET=554,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0181
PRIMER_LEFT_PENALTY=0.005674
PRIMER_RIGHT_PENALTY=0.012411
PRIMER_LEFT_SEQUENCE=CGTTTTCATGGAGGACTTTAGG
PRIMER_RIGHT_SEQUENCE=CTCCTCCTCACTTTCTTCCTCA
PRIMER_LEFT=339,22
PRIMER_RIGHT=630,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=0.0207
PRIMER_LEFT_1_PENALTY=0.008292
PRIMER_RIGHT_1_PENALTY=0.012411
PRIMER_LEFT_1_SEQUENCE=CAGTTCAAAAAGGAAAACCCTG
PRIMER_RIGHT_1_SEQUENCE=CTCCTCCTCACTTTCTTCCTCA
PRIMER_LEFT_1=364,22
PRIMER_RIGHT_1=630,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=59.988
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.0795
PRIMER_LEFT_2_PENALTY=0.005674
PRIMER_RIGHT_2_PENALTY=0.073866
PRIMER_LEFT_2_SEQUENCE=CGTTTTCATGGAGGACTTTAGG
PRIMER_RIGHT_2_SEQUENCE=TCATCATCCTCCTCCTCACTTT
PRIMER_LEFT_2=339,22
PRIMER_RIGHT_2=638,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.0795
PRIMER_LEFT_3_PENALTY=0.005674
PRIMER_RIGHT_3_PENALTY=0.073866
PRIMER_LEFT_3_SEQUENCE=CGTTTTCATGGAGGACTTTAGG
PRIMER_RIGHT_3_SEQUENCE=CATCATCCTCCTCCTCACTTTC
PRIMER_LEFT_3=339,22
PRIMER_RIGHT_3=637,22
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.0804
PRIMER_LEFT_4_PENALTY=0.005674
PRIMER_RIGHT_4_PENALTY=0.074695
PRIMER_LEFT_4_SEQUENCE=CGTTTTCATGGAGGACTTTAGG
PRIMER_RIGHT_4_SEQUENCE=ATCCTCCTCCTCACTTTCTTCC
PRIMER_LEFT_4=339,22
PRIMER_RIGHT_4=633,22
PRIMER_LEFT_4_TM=59.994
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_03206 Ps_contig_mira-and-tgicl-ass_franssen_29181
SEQUENCE=CTGCTAAGGCGAAGAGAAAAGGAAAAGGGAACGTGAATAATGAGAAGAAGCATGAAGTCCTATAACAAGAAACAAAGACTGAAGGTCCAGCTGCTGTTGAAGAAGAAGAAGAATCCGAGGAAATCAGAATCTGAGGTGCATGATGAGAATGATGATGAGGAAGAAAGTGAGGAGGAGGATGATGACGAGTAGAAAATTGGATGATGATAACATTTATGTAGGATGCTATGCTGATGATTTGATCATCTTATGTAGATGATTGTCATTTTATGAATAAAAAATCTNTCTTGCTACCCAAATATTTTGCTGCTCTTGGCTAATGCTTTGTCTGTTCAAAACGTGAATAGTTAAAATGGAGGGAGACTAAACTTTTATTGTACTTTAAGGCCAACTTCTTTAGTTAAGAGATTGTTTTATTCTTT
TARGET=99,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1141
PRIMER_LEFT_PENALTY=0.101683
PRIMER_RIGHT_PENALTY=0.012411
PRIMER_LEFT_SEQUENCE=AAGAGAAAAGGAAAAGGGAACG
PRIMER_RIGHT_SEQUENCE=CTCCTCCTCACTTTCTTCCTCA
PRIMER_LEFT=11,22
PRIMER_RIGHT=176,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.1685
PRIMER_LEFT_1_PENALTY=0.101683
PRIMER_RIGHT_1_PENALTY=0.066849
PRIMER_LEFT_1_SEQUENCE=AAGAGAAAAGGAAAAGGGAACG
PRIMER_RIGHT_1_SEQUENCE=TCAAATCATCAGCATAGCATCC
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=241,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.067
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.1764
PRIMER_LEFT_2_PENALTY=0.101683
PRIMER_RIGHT_2_PENALTY=0.074695
PRIMER_LEFT_2_SEQUENCE=AAGAGAAAAGGAAAAGGGAACG
PRIMER_RIGHT_2_SEQUENCE=ATCCTCCTCCTCACTTTCTTCC
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=179,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.1974
PRIMER_LEFT_3_PENALTY=0.101683
PRIMER_RIGHT_3_PENALTY=0.095713
PRIMER_LEFT_3_SEQUENCE=AAGAGAAAAGGAAAAGGGAACG
PRIMER_RIGHT_3_SEQUENCE=CTACTCGTCATCATCCTCCTCC
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=191,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.1974
PRIMER_LEFT_4_PENALTY=0.101683
PRIMER_RIGHT_4_PENALTY=0.095713
PRIMER_LEFT_4_SEQUENCE=AAGAGAAAAGGAAAAGGGAACG
PRIMER_RIGHT_4_SEQUENCE=TACTCGTCATCATCCTCCTCCT
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=190,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=180
=
PRIMER_SEQUENCE_ID=SSR_03207 Ps_contig_mira-and-tgicl-ass_franssen_29183
SEQUENCE=TTGATAATGCCTTATTGGAGTTTTTGGTTGAGTGAATTCAAGAGGACTGAGAGTATTGACCTTTCTAAGGATAGGCTTGCCTTGCAAAGGCTTCGCGAAGCTGCTGAGAAAGCTAAGATAGAACTGTCTTCAACATCTCAGACAGAGATAAACTTGCCTTTCATCACTGCTGATGCATCTGGTGCTAAGCATCTGAACATCACATTGACTAGATCTAAGTTTGAAGCTCTAGTAAATAACTTGATTGAAAGAACTAGGGCTCCATGTCAGAGCTGTTTGAAGGATGCAAACATATCTGTCAAGGACATTGACGAGGTTCTTCTTGTTGGAGGGATGACCCGTGTGCCTAAAGTCCAAGAGGTGGTTTCAGCGATATTTGGAAAGTCTCCCAGTAAAGGAGTAAATCCTGATGAGGCAGTTGCAATGGGAGCAGCACTCCAGGGTGGTATCCTACGTGGAGATGTTAAAGAGCTACTGCTTTTGGATGTAACACCCCTCTCTCTGGGTATTGAGACTTTGGGTGGTATCTTTACAAGGTTAATCAACCGTAACACTACTATTCCCACAAAAAAGAGTCAGGTCTTTTCAACGGCAGCTGACAATCAGACACAAGTAGGTATCAAGGTGTTACAAGGTGAGCGGGAAATGGCTACTGACAACAAAATGCTTGGAGAATTTGAGCTTGTTGGCATTCCTCCTTCTCCAAGAGGCATGCCTCAGATTGAGGTCACTTTTGACATTGATGCCAACGGTATTGTTACCGTGTCGGCCAAAGACAAGTCCACCGGTAAAGAACAACAAATCACTATTAGGTCGTCTGGTGGACTTTCAGATGATGAGATTGACAAGATGGTGAAAGAAGCAGAGTTACATGCTCAAAAAGATCAAGAAAGAAAGGCTCTCATCGATATTAGAAACAGTGCGGATACTTCCATCTATAGCATCGAGAAGAGCTTAGGTGAATATAGAGAGAAGATTCCTAGTGAAGTGGCCAAAGAAATTGAGGATGCAATTTCAGATTTGAGAAGTGCCATGTCAGGGGAGAATATTGACGAAATCAAGTCAAAGCTTGATGCGGCTAACAAAGCCGTCTCCAAGATTGGAGAGCACATGTCAGGTGGTTCTAGTGGCGGTCCCTCAGCTGGTGGTTCTCAAGGGGGGGACCAAGCTCCAGAAGCCGAATATGAAGAGGTGAAGAAATGATACTCAAAGCGGTTTTTTTATGGTTTTCCTTAGATAATTGTTTTAAGATGAGTTGTTTTTAGTTTAGTAGAGGAGTATATGGTCACTTATTTTGGTATCTAACGGTAAGATTTATTTATTTATACTGAAGGCTTTTTAATATGTAATGTTCTTGGAGAGTTGGATCACGCTGGTTAAATAATCATATTGAAATTTTTGCATAGTAGTCTATTCTCTGCTTGTTGATTGCAATTACTTTTTATTTTGCGCATGAAGGACTGTGT
TARGET=1313,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1971
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.101680
PRIMER_LEFT_SEQUENCE=AAGCCGAATATGAAGAGGTGAA
PRIMER_RIGHT_SEQUENCE=ATTTAACCAGCGTGATCCAACT
PRIMER_LEFT=1174,22
PRIMER_RIGHT=1381,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.3550
PRIMER_LEFT_1_PENALTY=0.253332
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=CAGAAGCCGAATATGAAGAGGT
PRIMER_RIGHT_1_SEQUENCE=ATTTAACCAGCGTGATCCAACT
PRIMER_LEFT_1=1171,22
PRIMER_RIGHT_1=1381,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.3550
PRIMER_LEFT_2_PENALTY=0.253332
PRIMER_RIGHT_2_PENALTY=0.101680
PRIMER_LEFT_2_SEQUENCE=AGCCGAATATGAAGAGGTGAAG
PRIMER_RIGHT_2_SEQUENCE=ATTTAACCAGCGTGATCCAACT
PRIMER_LEFT_2=1175,22
PRIMER_RIGHT_2=1381,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.3550
PRIMER_LEFT_3_PENALTY=0.253332
PRIMER_RIGHT_3_PENALTY=0.101680
PRIMER_LEFT_3_SEQUENCE=AGAAGCCGAATATGAAGAGGTG
PRIMER_RIGHT_3_SEQUENCE=ATTTAACCAGCGTGATCCAACT
PRIMER_LEFT_3=1172,22
PRIMER_RIGHT_3=1381,22
PRIMER_LEFT_3_TM=59.747
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=0.6835
PRIMER_LEFT_4_PENALTY=0.581808
PRIMER_RIGHT_4_PENALTY=0.101680
PRIMER_LEFT_4_SEQUENCE=GCCGAATATGAAGAGGTGAAGA
PRIMER_RIGHT_4_SEQUENCE=ATTTAACCAGCGTGATCCAACT
PRIMER_LEFT_4=1176,22
PRIMER_RIGHT_4=1381,22
PRIMER_LEFT_4_TM=60.582
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=206
=
PRIMER_SEQUENCE_ID=SSR_03208 Ps_contig_mira-and-tgicl-ass_franssen_29184
SEQUENCE=AACNCAAAAAAAAAAACAATGAAAATGGAGAGCGTACAGAGAAGTTTAAGCTTGTTGGTACTTTGTTTCCTGATCATGGGAACAACAATGTTAGTAAGTGGTCAGAGTGCAAACAACGTAAGAGCAACATACAATAACTACAACCCTCAGAACATAAACTGGGATTACAACAGAGCCAGTGTTTACTGTGCTACCTGGGATGCTAACCAGCCTTTGTCATGGCGTAGCAAATCGGTTGGACTGCCTTTTGTGGACCCGCCGGACCTACAGGCAGAGATTCTTGCGGCAAATGCTTGAGGGTGACAAACACTGCAACTGGAGCTCAGGTAACAGTGAGAATAGTGGATCAATGCTCTAATGGTGGACTGGACTTGGATGTGAATGTCTTCAATCAAATTGATACAAATGGAGGTGGCTACCAGGCGGGTCACCTTACAGTTAACTATGTCTTTGTTAATTGCTAAGGTCAATGAATCACTCTTTGTAATAATAAGAAATTCTAGCACTGCTTCCTAATAAAACCATAAATAAAACAAAACGGTGCTATACATGCATGCATGTTACATGTATAATTAATTGGATAGAAAAATAAATGATGATTACAATAATNCTTAGTTA
TARGET=549,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03209 Ps_contig_mira-and-tgicl-ass_franssen_29186
SEQUENCE=GGTAGTCAACGCAGAGTACNCGGNGRNGACACAAACAAACAATCACATAACTAGAAAATGGAGAGCACACAGAGAAGTTTAAGCTTGTTGGTACTTTGTTTCCTGCTTATGGGAACAACAATGTTAGTGAGTGGTCAGAGTGCAAACAACGTAAGAGCAACATACAATAACTACAACCCTCAGAACATAAACTGGGATTACAACAGAGCCAGTGTGTACTGTGCTACCTGGGATGCTAACCAGCCTTTGTCATGGCGTAGCCAATATGGTTGGACTGCCTTTTGTGGACCCGCCGGACCTACAGGCAGAGATTCTTGCGGCAAATGCTTGAGGGTGACAAACACTGCAACTGGAGCTCAGGTAACAGTGAGAATAGTGGATCAATGCTCTAATGGTGGACTGGACTTGGATGTGAATGTCTTCAATCAAATTGATACAAATGGAGCTGGCTACCAGGCGGGTCACCTTACGGTTAACTATGTCTTTGTTAATTGCTAAGGTCAATGAATCACTCTTTGTAATAATAAGAAATTCTAGCACTGCTTCCTAATAAAACCATAAAATAAAACAAAATGTGCTATACATGCATGTATGTTACATGTAATTAATTATGATANAAATAAATGATAGTAATTAACAAAACTAAAAAAAAAACCAAAAAAAAAAAAAAAAAAAAACGTAACTACTGTACGTTAGTAACTAACCACT
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03210 Ps_contig_mira-and-tgicl-ass_franssen_29189
SEQUENCE=GTCGGGGGTATTTGTTAATTTTGGGTCCCAATCCTTTTTGAAGGGGCCTTCAAATAAAACCCTAAACGTACCCGCGCGAGCAGCGTTGGCATCAGAAGCAAGTTGGATTGGAATTTGAGTCTCACTGAACTCAACGCCGCCGAGCTCGTCGTCGGCACCCAGAAACAGAAAGAAAGCTCGTTTTTTCAAATCCACTAGGAAAATGTCAGGTGAGGAAGCTGTTGTTGTAGCTGAGCCTGTTGCTGCTGCTGCACCTGGAATTCCAGGTGAGCCAATGGACATCATGACTGCCTTGCAACTTGTGCTCAGGAAGTCACTGGCATATGGAGGTCTTGCACGCGGCCTTCACGAAGGTGCAAAAGTAATTGAGAAGCACGCAGGACAACTTTGTGTTCTTGCTGAAGATTGTGACCAACCTGACTATGTTAAACTCGTCAAGGCTCTTTGCGCTGAGCACAACGTTAGCCTGCTGACAGTTCCCAGTGCAAAGACACTTGGAGAATGGGCTGGTTTGTGCAAAATTGATTCTGAGGGAAAAGCTAGGAAAGTTACTGGCTGCTCCTGTGTGGTCGTCAAGGATTTCGGGGAGGAGCATGAAGCCTACAATGTTGTTCTGCAGCATGTGAAAGCTCAATAAGTTGCAGGATCATGATGAGTGTCTGTTTCTTTCTTTTTCTTAGCTATTCCATCTGAATGAATTTTGCTTCTAGCTGGAGTTCTCTCCTAGGAATTTCAATTGTTTAAATATGATTTTGCTGTTTCCAGTTGTTATTGTTACTCTAATTTAGTTACTCACTTTTGTTCTCCTATGATATTTCTAAGAATTTAATCATCCACTCGTCTTTCACCAAACCGAATAC
TARGET=241,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0324
PRIMER_LEFT_PENALTY=0.019966
PRIMER_RIGHT_PENALTY=0.012470
PRIMER_LEFT_SEQUENCE=TGTTGTTGTAGCTGAGCCTGTT
PRIMER_RIGHT_SEQUENCE=GTCAGGTTGGTCACAATCTTCA
PRIMER_LEFT=219,22
PRIMER_RIGHT=420,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.1685
PRIMER_LEFT_1_PENALTY=0.019966
PRIMER_RIGHT_1_PENALTY=0.148509
PRIMER_LEFT_1_SEQUENCE=TGTTGTTGTAGCTGAGCCTGTT
PRIMER_RIGHT_1_SEQUENCE=TCAGGTTGGTCACAATCTTCAG
PRIMER_LEFT_1=219,22
PRIMER_RIGHT_1=419,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.1870
PRIMER_LEFT_2_PENALTY=0.174518
PRIMER_RIGHT_2_PENALTY=0.012470
PRIMER_LEFT_2_SEQUENCE=AATGTCAGGTGAGGAAGCTGTT
PRIMER_RIGHT_2_SEQUENCE=GTCAGGTTGGTCACAATCTTCA
PRIMER_LEFT_2=201,22
PRIMER_RIGHT_2=420,22
PRIMER_LEFT_2_TM=60.175
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.1870
PRIMER_LEFT_3_PENALTY=0.174518
PRIMER_RIGHT_3_PENALTY=0.012470
PRIMER_LEFT_3_SEQUENCE=AAATGTCAGGTGAGGAAGCTGT
PRIMER_RIGHT_3_SEQUENCE=GTCAGGTTGGTCACAATCTTCA
PRIMER_LEFT_3=200,22
PRIMER_RIGHT_3=420,22
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.2007
PRIMER_LEFT_4_PENALTY=0.019966
PRIMER_RIGHT_4_PENALTY=0.180765
PRIMER_LEFT_4_SEQUENCE=TGTTGTTGTAGCTGAGCCTGTT
PRIMER_RIGHT_4_SEQUENCE=CAAGAACACAAAGTTGTCCTGC
PRIMER_LEFT_4=219,22
PRIMER_RIGHT_4=397,22
PRIMER_LEFT_4_TM=59.980
PRIMER_RIGHT_4_TM=59.819
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_03211 Ps_contig_mira-and-tgicl-ass_franssen_29192
SEQUENCE=AAGTAATTAAAATATATAATTTATTTATTTAATAGTAATTTTAGCAATAATGGTATAGAAACGACATTAGCTATGGTAAGTTGTAACATAACATTGTAAGTAATATAAATACAGGCTTAAAAATTATGGACAGAAAACAAAAAACTAAAACAAAAAAGAAAAGAAATAGATTTTGGTTTGAAATTAACTTTCAAAACAGAAAACAAAAAATTCACAACCACACTGAACATGGTGATTCTCTTTTTTGTGTTTTAAAGTTTTCACAAACCAAAACCAATTTCTGTTTTGTCAAAAAAGAAAAACTATATGAGTTTTCCCTTCATCATCAGCAGTCCACCTATAAAATCTTGTGAAATTCACAATGTATGAAGGAAATCCCAATATTTGTTCAGCATGTGATCATTTGGCACAACTTCATCCCACTTTCCAGTACAAAATATAGCATAAGCATCCGCCGCATACTTGCCAACACTATGCAGTTCAGTTACATGGGTCCAACTCTCAGACAAGTACTCGCAAGAAAGGCGTTGCAGCATTCGCCCGTTACCTTTTTTCCTAGGAAACGTAACCCTTAAAGTTCGTTTCTTTTTAACTTACCTTCTT
TARGET=19,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03212 Ps_contig_mira-and-tgicl-ass_franssen_29198
SEQUENCE=AGTTTTAATTCTAAGATTAAGATCAACAAAGCTAAAAGTAACAAAGTAGCCACCTGNTNAGAATAAGGCTCAATAAATGAAATTAATAAGGTAAAACATAAAAACACCTACTGCACTGATTATTCCACAACGCCATTAACACTATCCCAAATTGATAGTAACCAATATCCTCACCACCTAAGGAAAAAAAATTGGCACATGAAATAAAGTATCTATGGGGTCTTCACTAGATGAGGAAATCAAAATAATAGGCACTCAAACCTTTATTATATGTAACATATGTGCTGAATAGAATAAATAAGAACTGTTGAATGAGAAAGAGAAGAAATGAATCAATCACCATGATCATTACAAACTGATTGATCATGTGACTAAGGTATATTTTGCTTGCTGAATGTATAATCAGTTTCCTGCGTCTCGACTGTAGAACTGATCGAGGGTTTTTGCCGGTATACGGTGAAGAAGCTCGCGAGGGAAAATGCGAAGCAAAGTCCATGCAAGGTCTAGAGACTGGAAGATGTTTCGGGTATCATAAGCTCCTTGCGCAACAAACTTCCTCTCAAACTTGTCCAGAAATTCCAGATACAGCAGATCCTCAGATGAAAGTGCTTCTTCTCCGACCACGGCTTTCATTGCTTGGACATCTTTTCCAATGGCATAATTTGCATAGAGCTGGTTGGACACATCAGAGTGATCCTTTCGTGTCATTCCCTCCCCAATGGCACTCTTCATTAAACGGGAAAGCGATGGAAGAACATTGATTGGTGGATATATCTGCCTGTTATGCAGCTGCCTGTCAATATATATCTGCCCCTCAGTGATATATCCGGTAAGATCAGGAGTTGGATGTGTAATATCATCATTAGGCATGGTTAGAATTGGAATTTGTGTGATAGAGCCTTTTCGCCCCTCGATTCTTCCAGCACGTTCGTATATTGTTGCAAGATCAGTATACATGTAACCAGGGTATCCACGTCTACCGGGCACTTCTTCTCGGGCAGCAGATACCTCACGAAGAGCATCAGCATAAGAACTCATATCTGTGAGTATCACAAGAACATGCTTGCCGCATTCATATGCCAAATATTCAGCAGTAGTAAGAGCAATACGAGGAGTGATGATACGCTCAATTGTAGGATCATTTGCCAGATTAAGGAAAAGGGTCACTCTCTCCATAGAGCCGTTCTCTTCAAAGTCACGTTTGAAAAACTGTGCAGTCTCCATGTTAACTCCCATGGCTGCAAACACAATAGCAAAATTGTCGTCTTCCCCGCCCTCGAGAAGATTATCGGATTTCTCTAGTCGCTTGACTAAGCCAGCCTGGCGACATATCTGTGCAGCAATTTCATTATGAGGAAGACCGGCAGCTGAGAAGAGAGGGATCTTTTGACCTCTAGCAATAGAATTCATGACATCAATTGTAGATATTCCCGTCTGAATCATCTCTTCTGGATAGGTTCTCTCACTAGGGTTGATGGAACTTCCTGATATATCCAAGTAAGCCTCAGGTAATATGGGTGGACCATTATCAATTGGTTTTCCTGAACCATTAAAAATGCGGCCAAGCATGTCCTGTGATACAGGAGTTTTTAACACCTCTCCAGTAAATTGCACAGTTGTGAATTTATTATCAATCCCAGATGTACCTTCAAAAACCTGAACAACAGCTTTTTCGCCATCAACTTCTAGAACTTGACCACGCCTAGTAGTTCCATCTCCTAACCGAATATTAACAATCTCTTGATACTTAGGTCCCTTAACTTTTTCGAGAATAACCAATGGTCCAGCAACCCCAGAAACAGTTCTATATTCCATTCCAATCTCCAAGGTTCCATCATCATCGTGAACGTTTTCAGCCGCACCCATTTCTGAAACTTGAAGCAAGAACCCTGTGTGTGTGATCTGAAGAGGAAGAGAAAGAGAAAGAGATAGGTGTTGGCGTTGTTGTGACAGATCTAAGAGATGATGGACCAAACAAATGCACGAACCATCAACGAAACAGCAATTCTTTGTCTCAAACAGATTGCACT
TARGET=1891,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0864
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.060824
PRIMER_LEFT_SEQUENCE=GACCACGCCTAGTAGTTCCATC
PRIMER_RIGHT_SEQUENCE=AACAACGCCAACACCTATCTCT
PRIMER_LEFT=1694,22
PRIMER_RIGHT=1945,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.0864
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.060824
PRIMER_LEFT_1_SEQUENCE=GACCACGCCTAGTAGTTCCATC
PRIMER_RIGHT_1_SEQUENCE=ACAACGCCAACACCTATCTCTT
PRIMER_LEFT_1=1694,22
PRIMER_RIGHT_1=1944,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=60.061
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=0.1177
PRIMER_LEFT_2_PENALTY=0.056850
PRIMER_RIGHT_2_PENALTY=0.060824
PRIMER_LEFT_2_SEQUENCE=CTTCTAGAACTTGACCACGCCT
PRIMER_RIGHT_2_SEQUENCE=AACAACGCCAACACCTATCTCT
PRIMER_LEFT_2=1682,22
PRIMER_RIGHT_2=1945,22
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=60.061
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=264
PRIMER_PAIR_PENALTY_3=0.1177
PRIMER_LEFT_3_PENALTY=0.056850
PRIMER_RIGHT_3_PENALTY=0.060824
PRIMER_LEFT_3_SEQUENCE=CTTCTAGAACTTGACCACGCCT
PRIMER_RIGHT_3_SEQUENCE=ACAACGCCAACACCTATCTCTT
PRIMER_LEFT_3=1682,22
PRIMER_RIGHT_3=1944,22
PRIMER_LEFT_3_TM=59.943
PRIMER_RIGHT_3_TM=60.061
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=0.2012
PRIMER_LEFT_4_PENALTY=0.025566
PRIMER_RIGHT_4_PENALTY=0.175676
PRIMER_LEFT_4_SEQUENCE=GACCACGCCTAGTAGTTCCATC
PRIMER_RIGHT_4_SEQUENCE=TTAGATCTGTCACAACAACGCC
PRIMER_LEFT_4=1694,22
PRIMER_RIGHT_4=1958,22
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=60.176
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_03213 Ps_contig_mira-and-tgicl-ass_franssen_29205
SEQUENCE=AGCATAAACTTCATCTACTCGTAAATAATTTAAACATCCATTTGATACAAAATGAGCAAGTGTATGAATTGTGTGCATGAGACAGTTCTCCAGCAGGTAGAGAGAGTCAAGAACATTTTCTCTCTAAAAAGACAACAAATTCAATTGCTAAGTTGTGTATGAGATCAACATAGGCAACAAAAATAATAACATAATATACACTTAAAATAAAATGAGCAAAAAAATAACAAGTACATAGAGTTACATAACTGCTGAATACATGAAGTAATACAGTAAAACTAGGTAGCAGCAACTTCTGCAGTTTTCTTATTAGCTTCCTTCAGTTTCTTCCAATTTTCTTCACTATCAATTATCTCTCCAGTTTTTATCAGGTAGCGAACTCTTTTACCATTTTCAAGAACTTTATGACCTACGCGGCTTGTTACATCCTTTTCTTTGGAGTAAAGCATCACATTTGAACTGTGGATAGGAGCTTCGACCTTATTTATTTGTCCGGGTTCTCCCTCTTGGTTACTTTTCACATGCTTTGTCTTCAAGTTTATGTCTTTAACAATGACACTGCTGTTGTGTTTGAAGATCTTAGTAATCTCCCCTATTTGACCCTTTTCATGTCCCGATATGACTTTCACTGTATCTCCAACTTTAACATGCAATTTGTGTAATACGGGAAGGCTGTTTGGTTTACATTCCTTTCGCTCCCATCGTTTAAGCTTCATTACAATAAGACATTTCTTCTCTGTTGGCTTACCCTGAAGAAAAGGGGAGAGAGTGATGGGAGAGAGAGGTTGGCCTAAGAAAGAGTTGGAGGAGAGGGAGAGTGAGGACATGGAACTCTGAAGAGAAGCCATAGCCATAGCTACCATTGTTGTTGTTGTATGTAACCTCTTGTTCTTTTTTGCTTCTGCTTCTGATAACGATTCTGGATAATATAAAATCTCAAAATAAAAGAGAAAGAGAGAGAAAATGAAGAGAATTTAAAATGGACTNT
TARGET=863,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1997
PRIMER_LEFT_PENALTY=0.047806
PRIMER_RIGHT_PENALTY=0.151911
PRIMER_LEFT_SEQUENCE=GGAGAGAGTGATGGGAGAGAGA
PRIMER_RIGHT_SEQUENCE=TCGTTATCAGAAGCAGAAGCAA
PRIMER_LEFT=761,22
PRIMER_RIGHT=916,22
PRIMER_LEFT_TM=59.952
PRIMER_RIGHT_TM=60.152
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=0.2027
PRIMER_LEFT_1_PENALTY=0.047806
PRIMER_RIGHT_1_PENALTY=0.154878
PRIMER_LEFT_1_SEQUENCE=GGAGAGAGTGATGGGAGAGAGA
PRIMER_RIGHT_1_SEQUENCE=TCCAGAATCGTTATCAGAAGCA
PRIMER_LEFT_1=761,22
PRIMER_RIGHT_1=923,22
PRIMER_LEFT_1_TM=59.952
PRIMER_RIGHT_1_TM=59.845
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.2657
PRIMER_LEFT_2_PENALTY=0.113803
PRIMER_RIGHT_2_PENALTY=0.151911
PRIMER_LEFT_2_SEQUENCE=TTGGCCTAAGAAAGAGTTGGAG
PRIMER_RIGHT_2_SEQUENCE=TCGTTATCAGAAGCAGAAGCAA
PRIMER_LEFT_2=785,22
PRIMER_RIGHT_2=916,22
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=60.152
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=0.2703
PRIMER_LEFT_3_PENALTY=0.118384
PRIMER_RIGHT_3_PENALTY=0.151911
PRIMER_LEFT_3_SEQUENCE=GGAGAGTGAGGACATGGAACTC
PRIMER_RIGHT_3_SEQUENCE=TCGTTATCAGAAGCAGAAGCAA
PRIMER_LEFT_3=812,22
PRIMER_RIGHT_3=916,22
PRIMER_LEFT_3_TM=60.118
PRIMER_RIGHT_3_TM=60.152
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.2706
PRIMER_LEFT_4_PENALTY=0.118715
PRIMER_RIGHT_4_PENALTY=0.151911
PRIMER_LEFT_4_SEQUENCE=AGAGTTGGAGGAGAGGGAGAGT
PRIMER_RIGHT_4_SEQUENCE=TCGTTATCAGAAGCAGAAGCAA
PRIMER_LEFT_4=797,22
PRIMER_RIGHT_4=916,22
PRIMER_LEFT_4_TM=59.881
PRIMER_RIGHT_4_TM=60.152
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_03214 Ps_contig_mira-and-tgicl-ass_franssen_29206
SEQUENCE=AAGCAGGGGTAGTCAACGCAAAGTACGCGGGGGTTATCAGAAGCAGAAGCAAAAAAGAACAAGAGGTTACATACAACAACAACAATGGTAGCTATGGCAATGGCTTCTCTTCAGAGTTCCATGTCCTCACTCTCCCTCTCCTCCAACTCTTTCTTAGGCCAACCTCTCTCTCCCATCACTCTCTCCCTTTCTTCAGGTAGCCAACAGAGAAGAAATGTCTTATTGTAATTAAGCTTAAACGAGTGGGAGGCGAAAGGAATGTAAACCAAACAGCCTTCCCGT
TARGET=73,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7530
PRIMER_LEFT_PENALTY=0.740565
PRIMER_RIGHT_PENALTY=0.012411
PRIMER_LEFT_SEQUENCE=GGGTTATCAGAAGCAGAAGCAA
PRIMER_RIGHT_SEQUENCE=CCTGAAGAAAGGGAGAGAGTGA
PRIMER_LEFT=30,22
PRIMER_RIGHT=196,22
PRIMER_LEFT_TM=60.741
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.7884
PRIMER_LEFT_1_PENALTY=0.740565
PRIMER_RIGHT_1_PENALTY=0.047806
PRIMER_LEFT_1_SEQUENCE=GGGTTATCAGAAGCAGAAGCAA
PRIMER_RIGHT_1_SEQUENCE=GGAGAGAGTGATGGGAGAGAGA
PRIMER_LEFT_1=30,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=60.741
PRIMER_RIGHT_1_TM=59.952
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.8544
PRIMER_LEFT_2_PENALTY=0.740565
PRIMER_RIGHT_2_PENALTY=0.113803
PRIMER_LEFT_2_SEQUENCE=GGGTTATCAGAAGCAGAAGCAA
PRIMER_RIGHT_2_SEQUENCE=TTGGCCTAAGAAAGAGTTGGAG
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=161,22
PRIMER_LEFT_2_TM=60.741
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=0.8589
PRIMER_LEFT_3_PENALTY=0.740565
PRIMER_RIGHT_3_PENALTY=0.118384
PRIMER_LEFT_3_SEQUENCE=GGGTTATCAGAAGCAGAAGCAA
PRIMER_RIGHT_3_SEQUENCE=GGAGAGTGAGGACATGGAACTC
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=134,22
PRIMER_LEFT_3_TM=60.741
PRIMER_RIGHT_3_TM=60.118
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.8593
PRIMER_LEFT_4_PENALTY=0.740565
PRIMER_RIGHT_4_PENALTY=0.118715
PRIMER_LEFT_4_SEQUENCE=GGGTTATCAGAAGCAGAAGCAA
PRIMER_RIGHT_4_SEQUENCE=AGAGTTGGAGGAGAGGGAGAGT
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=149,22
PRIMER_LEFT_4_TM=60.741
PRIMER_RIGHT_4_TM=59.881
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_03215 Ps_contig_mira-and-tgicl-ass_franssen_29211
SEQUENCE=GATATCATATACAAAAGTGAAGATTCAAATTCATGACATCCGACTTATTTATCTTTAAAAAATAAAATCTCGGCCACTAAACTACTTGATAAAAAAATTTATTATATCTATATAATTATATTTAAAAATGAGCGAATAGTGATAAAATTTGTCTAAACTATTATAAAAATAGTAAATATTAATTAAAATATTATAATTTTTAATTCTCAACTTATAAATATTAATAACTTATTTATTATAAATCATTTTTTACTTTTTATATATATTAAAAAATATAATAATTATGGTATAAAAATGAGTAAAATGTAATGGATTATGAGAAAATATATTATTTAAAAAGTAAAGTGATAATTGAATATGTGATTCTTACAAAATATGACTAACCAATAAAATAAGAAGAAACCTTAGAGGATAGGTAACCAAGTAAATATAATATAGTATAAATCTGGTAACACTAACAAGTGTTTCCCTGTGAGTCAGTAAGGTTACATGACAGTGGTACGTACGAGGCATGGGCTCATTTGTTAAATACAATTTCATTGTCAGACATGAGCTGTGATCTAAAAGATGTGAAATCGATAGAGAAAAACTCTCCACCAATTAAAATCAAACAATACGATGACATGGCATCACTATCATTCAGCCGCATCACGTAAAAACTTTCCGCATCCCTCGCACGTGACACCCATAACTCACTACTTTCCCATTATCCAACGCATGCCTCTTGGACACTCGTCCAATCAAGTTCCTCCACCTCACCCCTTTTTTTCCCCACCCCTATTCTATACTTAGCATGTTTAATTATTGAATTAGGATTCAATAATTATCCATTTATTAAATTAAATTAAAATTAATAGCCGACATGGAAAGTGAAGTTGAAAAAGAAAGTCATGTTTTCTCAGTAACAGTCATGTTTTCTGTTTAATTATGTGTCTGTTTTATCATTCATCATTTAACTGTCCTTTTTTTCTTATCTATTTTAGGAACAAAACCAAACCCACTTGCCCAAATTAGTTTTATTTTATTTTATTTTAAATTATAATATATAATAATAAATAAACAGCACCAACACCAAGTAGATGCTGTTGCTGAATGAATCTATCTAAGAGTTGGATTGGATTGGATGATGATTTGATTTTTTATTTTTATAATATTCTTTTATTTATCTTTATTCATTTCATTCCTTTCTTTCTCCCACACCTTCACATGGGAACACTATAAAATTCATATATCTTTATAGCATATGATGGCGGTCCCTTCTGTTCTCAGTCACAATTCTGTGTTGAATAATAATAATACTGAGCTTCCAGTTGTGGACTAAGGGCTCCATCTAGGTAGGAGACAAACAAGGCCGGTGATCCAGATTCACCATCTTATTTACTGTTGTTGCTGCTGCAGGTGCACGGTATGGTATGCTGGTCTCCCACTAATGGTTATTTTAACCCTTACCTTTAATACCTTTCTTTCTTCAACTTGTACTTGAACTTGAACTTTCACAACAGCCAAGTTTTTCCCTTTCAAGGTTAGTTAGTACTATTTTGCTTCTTGTGTTTATATTTCATCATGTGTCAGGGTTGTTGATTTGATTGTTTTCTATGATTGGGTTTTACTTCATAGATTGTGACTAGTTATTTTGGTAAAGCCTTTGATAATTTTGTCGTATTGATGATAGAGTTCAATAGTTTTGTAACATCATCGTATATGGTTTTGATTGACTTGGTTATCTTGTTTTTTTTACTGTTGTAAATGTAGTTTGTGTTAAAATGTCAACCACGAGGCCTAGCCAATCGTCCAACAATTCGGGGAGGTCAAGAAATAGTGCTAGGATTATTGCTCAGACGACTGTGGATGCAAAGCTTCATGCAACTTTTGAGGAGTCCGGTAGTTCGTTTGACTACTCGAGTTCGGTGCGTGTTTCTGGCTCGGTGGATGGAGATCAACAACCGAGGTCCAACAAAGTGACGACGGCTTACCTCAATCATATACAGAGAGGTAAGCAGATCCAGCCTTTCGGGTGCTTGCTAGCTTTAGATGAGAAAACGTGCAAGGTTGTTGCGTATAGTGAGAATGCGCCTGAGATGCTGACTATGGTGAGTCATGCTGTTCCTAGCGTTGGTGACCATCCTGCTCTTGGCATTGGAACTGACATAAGGACTGTTTTCACTGCGCCGAGTGCTTCTGCCTTGCAGAAGGCGCTAGGGTTTGCGGAGGTTTCACTTCTTAACCCGATTCTTGTTCATTGCAAGACTTCTGGGAAGCCGTTTTACGCGATCATTCATCGTGTTACTGGTAGTTTGATCATTGACTTTGAGCCGGTGAAGCCTTATGAAGTTCCCATGACTGCTGCGGGTGCCTTGCAATCTTACAAACTTGCTGCTAAAGCAATTACAAGATTGCAATCTTTGGCTAGTGGCAGCATGGAAAGGCTTTGTGATACCATGGTTCAAGAAGTTTTTGAACTAACGGGTTATGACAGGGTGATGGCTTATAAATTTCACGAGGATGATCACGGGGAGGTGATTGCTGAGATAGCAAAGCCAGGCCTAGAGCCATATCTAGGTCTGCACTATCCGGCGACAGATATTCCCCAGGCTGCGCGGTTTCTATTTATGAAGAACAAGGTCCGTATGATAGTTGATTGTAATGCAAAACATGTGAAGGTTCTTCAAGACGAAAAACTCCCATTTGATTTGACTCTGTGCGGTTCGACCTTGAGAGCTCCACATAGTTGCCATTTGCAGTACATGGCTAACATGGATTCAATTGCTTCGTTGGTTATGGCAGTTGTCGTCAATGACAGCGATGAAGATGGAGATAGCGCTGACGCAGTTCTCCCACAAAAGAAAAAGAGACTTTGGGGTTTGGTAGTTTGTCATAACACTACTCCAAGGTTTGTTCCTTTTCCTCTAAGGTATGCTTGTGAGTTTCTGGCTCAAGTGTTTGCCATCCATGTGAACAAAGAAATAGAGTTAGAATATCAGATTCTTGAGAAGAATATCCTGCGCACGCAGACGCTGTTGTGTGATATGTTGATGCGAGATGCACCCTTAGGTATTGTATCACAAAGCCCTAATATAATGGATCTAGTGAAATGTGATGGGGCTGCACTCTTTTATAGAAACAAGTTATGGTTATTAGGAGCGACACCGACTGAATCTCAATTAAGAGAGATAGCTTTATGGATGTCTGAGTATCATACAGATTCAACAGGTTTGAGTACAGACAGCTTGTCGGATGCAGGGTTTCCAGGGGCTCTTTCTCTTAGTGATACTGTATGTGGAATGGCAGCTGTTAGAATAACTTCAAAAGACATAGTTTTCTGGTTTAGGTCGCACACTGCTGCAGAAATCCGATGGGGTGGTGCAAAGCATGAACCGGGCGACCAAGACGATGGTAGGAAGATGCATCCAAGATCATCATTCAAGGCTTTCCTTGAAGTTGTGAAAGCCAGAAGCGTGCCGTGGAAAGACTTTGAAATGGATGCTATTCATTCGTTGCAGTTAATACTGAGAAATGCGTCCAAAGATACAGATATTATAGATTTGAATACGAAGGCAATCAATACAAGACTAAATGATTTGAAGATTGAAGGGATGCAGGAATTGGAAGCAGTGACAAGTGAGATGGTTAGATTAATTGAAACAGCAACAGTGCCTATTTTGGCAGTGGATGTTGATGGGACGGTCAACGGATGGAATATAAAAATCGCCGAGTTGACAGGTCTTCCGGTTGGCGAAGCTATTGGAAAACATCTACTCACCCTGGTTGAGGATTCTTCAACTGATATAGTCAAGAAGATGCTCAACTTGGCACTGCAGGGTATGCCTTTTTTCCTTTTCCATCTTATTATCCTTTACTTAAGACTCTAACTGTTGATTTTGTTCTCTTATAATGGCACTGCAGGGTGAAGAAGAGAAGAATGTTCAATTCGAGATAAAAACACATGGGGATCAGGTGGAATCCGGTCCTATTAGTTTGATAGTTAACGCATGTGCAAGCAAGGATCTTCGTGAAAATGTAGTGGGGGTTTGTTTTGTGGCCCAAGATATAACTGCTCAGAAGACTGTCATGGACAAATTCACCCGAATCGAAGGCGATTACAAAGCAATTGTGCAGAACCCAAATCAGTTGATCCCTCCTATATTCGGTACAGATGAATTTGGCTGGTGTTGTGAGTGGAATGCAGCTATGATTAAGTTAACCGGATGGAAGCGCGAGGAGGTGATGGATAAAATGCTTCTCGGAGAGGTTTTCGGAACTCAAATGTCTTGTTGTCGTCTAAAGAATCAAGAAGCTTTTGTTAATTTCGGCATTGTACTTAATAAAGCCATGACCGGTTTGGAAACAGAAAAGGTCCCTTTTGGCTTCTTCTCTCGAAAAGGCAAGTATGTAGAGTGCCTACTCTCGGTGAGTAAGAAAATCGACGCAGAGGGCCTAGTTACCGGAGTCTTCTGTTTCTTGCAGTTAGCTAGCCCTGAGCTGCAACAAGCATTACATATTCAGCGCCTGTCCGAACAAACCGCTCTCAAGAGACTGAAAGTACTAACTTACATGAAAAGGCAGATCAGGAATCCGTTGGCTGGGATCGTGTTTTCCAGTAAAATGCTGGAGGGTACTGACTTGGAAACCGAACAAAAGCGAATCGTGAACACTAGTTCTCAGTGCCAACGCCAGCTTAGCAAAATTCTTGACGACTCTGATCTCGACGGCATCATTGATGGGTACTTGGATCTTGAGATGGCTGAATTCACTTTCCAACGCCAGCTTAGCAAAATTCTTGACGACTCTGATCTCGACGGCATCATTGATGGGTACTTGGATCTTGAGATGGCTGAATTCACTTTACATGAGGTACTGGTTACCTCTCTTAGTCAAGTCATGAATAGGAGTAACACAAAGGGTATCCGTATAGCCAACGATGTTGCGGAGCATATCGCGAGGGAAACCTTGTATGGTGATAGTCTTAGGCTTCAGCAGGTCCTAGCTGACTTTTTACTAATTTCCATCAATTCCACACCTAATGGAGGCCAGGTTGTTATAGCAGCCTCCTTAACTAAAGAACAGTTGGGAAAATCTGTCCATCTTGTTAACTTGGAGCTCAGGTAACTGTTCCATATTCACGCATGTATCGATTTTCAGTATCCGATGATTTAAGCCTCGGTAATAATCTTAGCTTTGGTGAAACTGCTAACTGCAATAAACTANTATCTGTATATATTGTTCATTTGCGATTTGTGCTCAGCATAACACACGGTGGTAGTGGCGTGCCAGAAGCGGCGCTGAACCAGATGTTTGGAAATAATGTGCTAGAATCTGAGGAGGGTATTAGCCTACACATCAGTCGGAAGTTGTTAAAGCTTATGAATGGAGATGTTCGTTATTTAAAAGAAGCAGGAAAATCATCGTTTATTCTATCTGTTGAACTTGCAGCAGCTCATAAGTTGAAAGGTTGAAATTTTGGAAAAATAATAAAAGTGAACACGGCATGCTATTGTTCAATGTAACTCTTATTTTGTTTCATGTTTCTTTAACTTGTTGTAACGTATGTTGTAGATATGTGTATTAGTGTCTCTTTCACACTCGTGGCCTATTCTTATTTCTTAGTCATGCTTTATGTTTTTTGTTTTATTTTTAGAGCAATTAATGGCATGTGTGGGGATGAACGCAGTTGGGGAAAACAGTTAATAACAACTATGCTATGACTTACTGTACTAGTTTCTCAATATCTTCTTTCTCTGCTTTATTCAGTCCTGTCACCCATACTATATATAGTACACATATATAGTACAATCTAGGAAGAGTTTAAAGAGTACATTCCACCCAATTACAAGTTCTGAATAACAAACTAGTGACTCTTTTAACAATCTTGATTACTCACAGCACTGACAAATAAAAGCTTCCTACCCCAGATTTGAGCTACTTGTACCTCATTGCATTTCTCTTTGCCTCGCAATTGTGGAATGGACTCCTCCTCTTCTGACCCTATCCTTTCCAAATCTTCCTCTTCATAGTTCATGACATCCCGAGTCCATCAAAACTGACCTTGTCCTTAAGGTCAAAGGTCGGGTAAATAGCATATATGTCTCTCAAAACTTGGTTTTGATCATTAATAGTGTCTTTCTTCCAATCCATCACAGCTAACGGCTTGATGTGAGGCCGGTATCCAAGAGGCATATCCAAGGTTTTCATAGTTGTATCAAAATAGGGTTTCAAGTTTCAACTGTGATATATGGAACATAAGACGAATCCTATGGGTGC
TARGET=834,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1603
PRIMER_LEFT_PENALTY=0.038090
PRIMER_RIGHT_PENALTY=0.122257
PRIMER_LEFT_SEQUENCE=ACACTCGTCCAATCAAGTTCCT
PRIMER_RIGHT_SEQUENCE=GTGGGTTTGGTTTTGTTCCTAA
PRIMER_LEFT=728,22
PRIMER_RIGHT=1000,22
PRIMER_LEFT_TM=60.038
PRIMER_RIGHT_TM=60.122
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=273
PRIMER_PAIR_PENALTY_1=0.1846
PRIMER_LEFT_1_PENALTY=0.038090
PRIMER_RIGHT_1_PENALTY=0.146525
PRIMER_LEFT_1_SEQUENCE=ACACTCGTCCAATCAAGTTCCT
PRIMER_RIGHT_1_SEQUENCE=TTCAACTTCACTTTCCATGTCG
PRIMER_LEFT_1=728,22
PRIMER_RIGHT_1=879,22
PRIMER_LEFT_1_TM=60.038
PRIMER_RIGHT_1_TM=60.147
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.1933
PRIMER_LEFT_2_PENALTY=0.046801
PRIMER_RIGHT_2_PENALTY=0.146525
PRIMER_LEFT_2_SEQUENCE=CACGTGACACCCATAACTCACT
PRIMER_RIGHT_2_SEQUENCE=TTCAACTTCACTTTCCATGTCG
PRIMER_LEFT_2=675,22
PRIMER_RIGHT_2=879,22
PRIMER_LEFT_2_TM=59.953
PRIMER_RIGHT_2_TM=60.147
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.2052
PRIMER_LEFT_3_PENALTY=0.082963
PRIMER_RIGHT_3_PENALTY=0.122257
PRIMER_LEFT_3_SEQUENCE=TTTTTCCCCACCCCTATTCTAT
PRIMER_RIGHT_3_SEQUENCE=GTGGGTTTGGTTTTGTTCCTAA
PRIMER_LEFT_3=764,22
PRIMER_RIGHT_3=1000,22
PRIMER_LEFT_3_TM=59.917
PRIMER_RIGHT_3_TM=60.122
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=0.2295
PRIMER_LEFT_4_PENALTY=0.082963
PRIMER_RIGHT_4_PENALTY=0.146525
PRIMER_LEFT_4_SEQUENCE=TTTTTCCCCACCCCTATTCTAT
PRIMER_RIGHT_4_SEQUENCE=TTCAACTTCACTTTCCATGTCG
PRIMER_LEFT_4=764,22
PRIMER_RIGHT_4=879,22
PRIMER_LEFT_4_TM=59.917
PRIMER_RIGHT_4_TM=60.147
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=116
=
PRIMER_SEQUENCE_ID=SSR_03216 Ps_contig_mira-and-tgicl-ass_franssen_29211
SEQUENCE=GATATCATATACAAAAGTGAAGATTCAAATTCATGACATCCGACTTATTTATCTTTAAAAAATAAAATCTCGGCCACTAAACTACTTGATAAAAAAATTTATTATATCTATATAATTATATTTAAAAATGAGCGAATAGTGATAAAATTTGTCTAAACTATTATAAAAATAGTAAATATTAATTAAAATATTATAATTTTTAATTCTCAACTTATAAATATTAATAACTTATTTATTATAAATCATTTTTTACTTTTTATATATATTAAAAAATATAATAATTATGGTATAAAAATGAGTAAAATGTAATGGATTATGAGAAAATATATTATTTAAAAAGTAAAGTGATAATTGAATATGTGATTCTTACAAAATATGACTAACCAATAAAATAAGAAGAAACCTTAGAGGATAGGTAACCAAGTAAATATAATATAGTATAAATCTGGTAACACTAACAAGTGTTTCCCTGTGAGTCAGTAAGGTTACATGACAGTGGTACGTACGAGGCATGGGCTCATTTGTTAAATACAATTTCATTGTCAGACATGAGCTGTGATCTAAAAGATGTGAAATCGATAGAGAAAAACTCTCCACCAATTAAAATCAAACAATACGATGACATGGCATCACTATCATTCAGCCGCATCACGTAAAAACTTTCCGCATCCCTCGCACGTGACACCCATAACTCACTACTTTCCCATTATCCAACGCATGCCTCTTGGACACTCGTCCAATCAAGTTCCTCCACCTCACCCCTTTTTTTCCCCACCCCTATTCTATACTTAGCATGTTTAATTATTGAATTAGGATTCAATAATTATCCATTTATTAAATTAAATTAAAATTAATAGCCGACATGGAAAGTGAAGTTGAAAAAGAAAGTCATGTTTTCTCAGTAACAGTCATGTTTTCTGTTTAATTATGTGTCTGTTTTATCATTCATCATTTAACTGTCCTTTTTTTCTTATCTATTTTAGGAACAAAACCAAACCCACTTGCCCAAATTAGTTTTATTTTATTTTATTTTAAATTATAATATATAATAATAAATAAACAGCACCAACACCAAGTAGATGCTGTTGCTGAATGAATCTATCTAAGAGTTGGATTGGATTGGATGATGATTTGATTTTTTATTTTTATAATATTCTTTTATTTATCTTTATTCATTTCATTCCTTTCTTTCTCCCACACCTTCACATGGGAACACTATAAAATTCATATATCTTTATAGCATATGATGGCGGTCCCTTCTGTTCTCAGTCACAATTCTGTGTTGAATAATAATAATACTGAGCTTCCAGTTGTGGACTAAGGGCTCCATCTAGGTAGGAGACAAACAAGGCCGGTGATCCAGATTCACCATCTTATTTACTGTTGTTGCTGCTGCAGGTGCACGGTATGGTATGCTGGTCTCCCACTAATGGTTATTTTAACCCTTACCTTTAATACCTTTCTTTCTTCAACTTGTACTTGAACTTGAACTTTCACAACAGCCAAGTTTTTCCCTTTCAAGGTTAGTTAGTACTATTTTGCTTCTTGTGTTTATATTTCATCATGTGTCAGGGTTGTTGATTTGATTGTTTTCTATGATTGGGTTTTACTTCATAGATTGTGACTAGTTATTTTGGTAAAGCCTTTGATAATTTTGTCGTATTGATGATAGAGTTCAATAGTTTTGTAACATCATCGTATATGGTTTTGATTGACTTGGTTATCTTGTTTTTTTTACTGTTGTAAATGTAGTTTGTGTTAAAATGTCAACCACGAGGCCTAGCCAATCGTCCAACAATTCGGGGAGGTCAAGAAATAGTGCTAGGATTATTGCTCAGACGACTGTGGATGCAAAGCTTCATGCAACTTTTGAGGAGTCCGGTAGTTCGTTTGACTACTCGAGTTCGGTGCGTGTTTCTGGCTCGGTGGATGGAGATCAACAACCGAGGTCCAACAAAGTGACGACGGCTTACCTCAATCATATACAGAGAGGTAAGCAGATCCAGCCTTTCGGGTGCTTGCTAGCTTTAGATGAGAAAACGTGCAAGGTTGTTGCGTATAGTGAGAATGCGCCTGAGATGCTGACTATGGTGAGTCATGCTGTTCCTAGCGTTGGTGACCATCCTGCTCTTGGCATTGGAACTGACATAAGGACTGTTTTCACTGCGCCGAGTGCTTCTGCCTTGCAGAAGGCGCTAGGGTTTGCGGAGGTTTCACTTCTTAACCCGATTCTTGTTCATTGCAAGACTTCTGGGAAGCCGTTTTACGCGATCATTCATCGTGTTACTGGTAGTTTGATCATTGACTTTGAGCCGGTGAAGCCTTATGAAGTTCCCATGACTGCTGCGGGTGCCTTGCAATCTTACAAACTTGCTGCTAAAGCAATTACAAGATTGCAATCTTTGGCTAGTGGCAGCATGGAAAGGCTTTGTGATACCATGGTTCAAGAAGTTTTTGAACTAACGGGTTATGACAGGGTGATGGCTTATAAATTTCACGAGGATGATCACGGGGAGGTGATTGCTGAGATAGCAAAGCCAGGCCTAGAGCCATATCTAGGTCTGCACTATCCGGCGACAGATATTCCCCAGGCTGCGCGGTTTCTATTTATGAAGAACAAGGTCCGTATGATAGTTGATTGTAATGCAAAACATGTGAAGGTTCTTCAAGACGAAAAACTCCCATTTGATTTGACTCTGTGCGGTTCGACCTTGAGAGCTCCACATAGTTGCCATTTGCAGTACATGGCTAACATGGATTCAATTGCTTCGTTGGTTATGGCAGTTGTCGTCAATGACAGCGATGAAGATGGAGATAGCGCTGACGCAGTTCTCCCACAAAAGAAAAAGAGACTTTGGGGTTTGGTAGTTTGTCATAACACTACTCCAAGGTTTGTTCCTTTTCCTCTAAGGTATGCTTGTGAGTTTCTGGCTCAAGTGTTTGCCATCCATGTGAACAAAGAAATAGAGTTAGAATATCAGATTCTTGAGAAGAATATCCTGCGCACGCAGACGCTGTTGTGTGATATGTTGATGCGAGATGCACCCTTAGGTATTGTATCACAAAGCCCTAATATAATGGATCTAGTGAAATGTGATGGGGCTGCACTCTTTTATAGAAACAAGTTATGGTTATTAGGAGCGACACCGACTGAATCTCAATTAAGAGAGATAGCTTTATGGATGTCTGAGTATCATACAGATTCAACAGGTTTGAGTACAGACAGCTTGTCGGATGCAGGGTTTCCAGGGGCTCTTTCTCTTAGTGATACTGTATGTGGAATGGCAGCTGTTAGAATAACTTCAAAAGACATAGTTTTCTGGTTTAGGTCGCACACTGCTGCAGAAATCCGATGGGGTGGTGCAAAGCATGAACCGGGCGACCAAGACGATGGTAGGAAGATGCATCCAAGATCATCATTCAAGGCTTTCCTTGAAGTTGTGAAAGCCAGAAGCGTGCCGTGGAAAGACTTTGAAATGGATGCTATTCATTCGTTGCAGTTAATACTGAGAAATGCGTCCAAAGATACAGATATTATAGATTTGAATACGAAGGCAATCAATACAAGACTAAATGATTTGAAGATTGAAGGGATGCAGGAATTGGAAGCAGTGACAAGTGAGATGGTTAGATTAATTGAAACAGCAACAGTGCCTATTTTGGCAGTGGATGTTGATGGGACGGTCAACGGATGGAATATAAAAATCGCCGAGTTGACAGGTCTTCCGGTTGGCGAAGCTATTGGAAAACATCTACTCACCCTGGTTGAGGATTCTTCAACTGATATAGTCAAGAAGATGCTCAACTTGGCACTGCAGGGTATGCCTTTTTTCCTTTTCCATCTTATTATCCTTTACTTAAGACTCTAACTGTTGATTTTGTTCTCTTATAATGGCACTGCAGGGTGAAGAAGAGAAGAATGTTCAATTCGAGATAAAAACACATGGGGATCAGGTGGAATCCGGTCCTATTAGTTTGATAGTTAACGCATGTGCAAGCAAGGATCTTCGTGAAAATGTAGTGGGGGTTTGTTTTGTGGCCCAAGATATAACTGCTCAGAAGACTGTCATGGACAAATTCACCCGAATCGAAGGCGATTACAAAGCAATTGTGCAGAACCCAAATCAGTTGATCCCTCCTATATTCGGTACAGATGAATTTGGCTGGTGTTGTGAGTGGAATGCAGCTATGATTAAGTTAACCGGATGGAAGCGCGAGGAGGTGATGGATAAAATGCTTCTCGGAGAGGTTTTCGGAACTCAAATGTCTTGTTGTCGTCTAAAGAATCAAGAAGCTTTTGTTAATTTCGGCATTGTACTTAATAAAGCCATGACCGGTTTGGAAACAGAAAAGGTCCCTTTTGGCTTCTTCTCTCGAAAAGGCAAGTATGTAGAGTGCCTACTCTCGGTGAGTAAGAAAATCGACGCAGAGGGCCTAGTTACCGGAGTCTTCTGTTTCTTGCAGTTAGCTAGCCCTGAGCTGCAACAAGCATTACATATTCAGCGCCTGTCCGAACAAACCGCTCTCAAGAGACTGAAAGTACTAACTTACATGAAAAGGCAGATCAGGAATCCGTTGGCTGGGATCGTGTTTTCCAGTAAAATGCTGGAGGGTACTGACTTGGAAACCGAACAAAAGCGAATCGTGAACACTAGTTCTCAGTGCCAACGCCAGCTTAGCAAAATTCTTGACGACTCTGATCTCGACGGCATCATTGATGGGTACTTGGATCTTGAGATGGCTGAATTCACTTTCCAACGCCAGCTTAGCAAAATTCTTGACGACTCTGATCTCGACGGCATCATTGATGGGTACTTGGATCTTGAGATGGCTGAATTCACTTTACATGAGGTACTGGTTACCTCTCTTAGTCAAGTCATGAATAGGAGTAACACAAAGGGTATCCGTATAGCCAACGATGTTGCGGAGCATATCGCGAGGGAAACCTTGTATGGTGATAGTCTTAGGCTTCAGCAGGTCCTAGCTGACTTTTTACTAATTTCCATCAATTCCACACCTAATGGAGGCCAGGTTGTTATAGCAGCCTCCTTAACTAAAGAACAGTTGGGAAAATCTGTCCATCTTGTTAACTTGGAGCTCAGGTAACTGTTCCATATTCACGCATGTATCGATTTTCAGTATCCGATGATTTAAGCCTCGGTAATAATCTTAGCTTTGGTGAAACTGCTAACTGCAATAAACTANTATCTGTATATATTGTTCATTTGCGATTTGTGCTCAGCATAACACACGGTGGTAGTGGCGTGCCAGAAGCGGCGCTGAACCAGATGTTTGGAAATAATGTGCTAGAATCTGAGGAGGGTATTAGCCTACACATCAGTCGGAAGTTGTTAAAGCTTATGAATGGAGATGTTCGTTATTTAAAAGAAGCAGGAAAATCATCGTTTATTCTATCTGTTGAACTTGCAGCAGCTCATAAGTTGAAAGGTTGAAATTTTGGAAAAATAATAAAAGTGAACACGGCATGCTATTGTTCAATGTAACTCTTATTTTGTTTCATGTTTCTTTAACTTGTTGTAACGTATGTTGTAGATATGTGTATTAGTGTCTCTTTCACACTCGTGGCCTATTCTTATTTCTTAGTCATGCTTTATGTTTTTTGTTTTATTTTTAGAGCAATTAATGGCATGTGTGGGGATGAACGCAGTTGGGGAAAACAGTTAATAACAACTATGCTATGACTTACTGTACTAGTTTCTCAATATCTTCTTTCTCTGCTTTATTCAGTCCTGTCACCCATACTATATATAGTACACATATATAGTACAATCTAGGAAGAGTTTAAAGAGTACATTCCACCCAATTACAAGTTCTGAATAACAAACTAGTGACTCTTTTAACAATCTTGATTACTCACAGCACTGACAAATAAAAGCTTCCTACCCCAGATTTGAGCTACTTGTACCTCATTGCATTTCTCTTTGCCTCGCAATTGTGGAATGGACTCCTCCTCTTCTGACCCTATCCTTTCCAAATCTTCCTCTTCATAGTTCATGACATCCCGAGTCCATCAAAACTGACCTTGTCCTTAAGGTCAAAGGTCGGGTAAATAGCATATATGTCTCTCAAAACTTGGTTTTGATCATTAATAGTGTCTTTCTTCCAATCCATCACAGCTAACGGCTTGATGTGAGGCCGGTATCCAAGAGGCATATCCAAGGTTTTCATAGTTGTATCAAAATAGGGTTTCAAGTTTCAACTGTGATATATGGAACATAAGACGAATCCTATGGGTGC
TARGET=1015,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1458
PRIMER_LEFT_PENALTY=0.122257
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=TTAGGAACAAAACCAAACCCAC
PRIMER_RIGHT_SEQUENCE=GTGTGGGAGAAAGAAAGGAATG
PRIMER_LEFT=979,22
PRIMER_RIGHT=1199,22
PRIMER_LEFT_TM=60.122
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.1465
PRIMER_LEFT_1_PENALTY=0.122257
PRIMER_RIGHT_1_PENALTY=0.024206
PRIMER_LEFT_1_SEQUENCE=TTAGGAACAAAACCAAACCCAC
PRIMER_RIGHT_1_SEQUENCE=ATCATCCAATCCAATCCAACTC
PRIMER_LEFT_1=979,22
PRIMER_RIGHT_1=1127,22
PRIMER_LEFT_1_TM=60.122
PRIMER_RIGHT_1_TM=60.024
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.1797
PRIMER_LEFT_2_PENALTY=0.122257
PRIMER_RIGHT_2_PENALTY=0.057411
PRIMER_LEFT_2_SEQUENCE=TTAGGAACAAAACCAAACCCAC
PRIMER_RIGHT_2_SEQUENCE=TCAGCAACAGCATCTACTTGGT
PRIMER_LEFT_2=979,22
PRIMER_RIGHT_2=1091,22
PRIMER_LEFT_2_TM=60.122
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=0.2039
PRIMER_LEFT_3_PENALTY=0.146525
PRIMER_RIGHT_3_PENALTY=0.057411
PRIMER_LEFT_3_SEQUENCE=CGACATGGAAAGTGAAGTTGAA
PRIMER_RIGHT_3_SEQUENCE=TCAGCAACAGCATCTACTTGGT
PRIMER_LEFT_3=858,22
PRIMER_RIGHT_3=1091,22
PRIMER_LEFT_3_TM=60.147
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.2382
PRIMER_LEFT_4_PENALTY=0.122257
PRIMER_RIGHT_4_PENALTY=0.115965
PRIMER_LEFT_4_SEQUENCE=TTAGGAACAAAACCAAACCCAC
PRIMER_RIGHT_4_SEQUENCE=TTGTGACTGAGAACAGAAGGGA
PRIMER_LEFT_4=979,22
PRIMER_RIGHT_4=1274,22
PRIMER_LEFT_4_TM=60.122
PRIMER_RIGHT_4_TM=59.884
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_03217 Ps_contig_mira-and-tgicl-ass_franssen_29211
SEQUENCE=GATATCATATACAAAAGTGAAGATTCAAATTCATGACATCCGACTTATTTATCTTTAAAAAATAAAATCTCGGCCACTAAACTACTTGATAAAAAAATTTATTATATCTATATAATTATATTTAAAAATGAGCGAATAGTGATAAAATTTGTCTAAACTATTATAAAAATAGTAAATATTAATTAAAATATTATAATTTTTAATTCTCAACTTATAAATATTAATAACTTATTTATTATAAATCATTTTTTACTTTTTATATATATTAAAAAATATAATAATTATGGTATAAAAATGAGTAAAATGTAATGGATTATGAGAAAATATATTATTTAAAAAGTAAAGTGATAATTGAATATGTGATTCTTACAAAATATGACTAACCAATAAAATAAGAAGAAACCTTAGAGGATAGGTAACCAAGTAAATATAATATAGTATAAATCTGGTAACACTAACAAGTGTTTCCCTGTGAGTCAGTAAGGTTACATGACAGTGGTACGTACGAGGCATGGGCTCATTTGTTAAATACAATTTCATTGTCAGACATGAGCTGTGATCTAAAAGATGTGAAATCGATAGAGAAAAACTCTCCACCAATTAAAATCAAACAATACGATGACATGGCATCACTATCATTCAGCCGCATCACGTAAAAACTTTCCGCATCCCTCGCACGTGACACCCATAACTCACTACTTTCCCATTATCCAACGCATGCCTCTTGGACACTCGTCCAATCAAGTTCCTCCACCTCACCCCTTTTTTTCCCCACCCCTATTCTATACTTAGCATGTTTAATTATTGAATTAGGATTCAATAATTATCCATTTATTAAATTAAATTAAAATTAATAGCCGACATGGAAAGTGAAGTTGAAAAAGAAAGTCATGTTTTCTCAGTAACAGTCATGTTTTCTGTTTAATTATGTGTCTGTTTTATCATTCATCATTTAACTGTCCTTTTTTTCTTATCTATTTTAGGAACAAAACCAAACCCACTTGCCCAAATTAGTTTTATTTTATTTTATTTTAAATTATAATATATAATAATAAATAAACAGCACCAACACCAAGTAGATGCTGTTGCTGAATGAATCTATCTAAGAGTTGGATTGGATTGGATGATGATTTGATTTTTTATTTTTATAATATTCTTTTATTTATCTTTATTCATTTCATTCCTTTCTTTCTCCCACACCTTCACATGGGAACACTATAAAATTCATATATCTTTATAGCATATGATGGCGGTCCCTTCTGTTCTCAGTCACAATTCTGTGTTGAATAATAATAATACTGAGCTTCCAGTTGTGGACTAAGGGCTCCATCTAGGTAGGAGACAAACAAGGCCGGTGATCCAGATTCACCATCTTATTTACTGTTGTTGCTGCTGCAGGTGCACGGTATGGTATGCTGGTCTCCCACTAATGGTTATTTTAACCCTTACCTTTAATACCTTTCTTTCTTCAACTTGTACTTGAACTTGAACTTTCACAACAGCCAAGTTTTTCCCTTTCAAGGTTAGTTAGTACTATTTTGCTTCTTGTGTTTATATTTCATCATGTGTCAGGGTTGTTGATTTGATTGTTTTCTATGATTGGGTTTTACTTCATAGATTGTGACTAGTTATTTTGGTAAAGCCTTTGATAATTTTGTCGTATTGATGATAGAGTTCAATAGTTTTGTAACATCATCGTATATGGTTTTGATTGACTTGGTTATCTTGTTTTTTTTACTGTTGTAAATGTAGTTTGTGTTAAAATGTCAACCACGAGGCCTAGCCAATCGTCCAACAATTCGGGGAGGTCAAGAAATAGTGCTAGGATTATTGCTCAGACGACTGTGGATGCAAAGCTTCATGCAACTTTTGAGGAGTCCGGTAGTTCGTTTGACTACTCGAGTTCGGTGCGTGTTTCTGGCTCGGTGGATGGAGATCAACAACCGAGGTCCAACAAAGTGACGACGGCTTACCTCAATCATATACAGAGAGGTAAGCAGATCCAGCCTTTCGGGTGCTTGCTAGCTTTAGATGAGAAAACGTGCAAGGTTGTTGCGTATAGTGAGAATGCGCCTGAGATGCTGACTATGGTGAGTCATGCTGTTCCTAGCGTTGGTGACCATCCTGCTCTTGGCATTGGAACTGACATAAGGACTGTTTTCACTGCGCCGAGTGCTTCTGCCTTGCAGAAGGCGCTAGGGTTTGCGGAGGTTTCACTTCTTAACCCGATTCTTGTTCATTGCAAGACTTCTGGGAAGCCGTTTTACGCGATCATTCATCGTGTTACTGGTAGTTTGATCATTGACTTTGAGCCGGTGAAGCCTTATGAAGTTCCCATGACTGCTGCGGGTGCCTTGCAATCTTACAAACTTGCTGCTAAAGCAATTACAAGATTGCAATCTTTGGCTAGTGGCAGCATGGAAAGGCTTTGTGATACCATGGTTCAAGAAGTTTTTGAACTAACGGGTTATGACAGGGTGATGGCTTATAAATTTCACGAGGATGATCACGGGGAGGTGATTGCTGAGATAGCAAAGCCAGGCCTAGAGCCATATCTAGGTCTGCACTATCCGGCGACAGATATTCCCCAGGCTGCGCGGTTTCTATTTATGAAGAACAAGGTCCGTATGATAGTTGATTGTAATGCAAAACATGTGAAGGTTCTTCAAGACGAAAAACTCCCATTTGATTTGACTCTGTGCGGTTCGACCTTGAGAGCTCCACATAGTTGCCATTTGCAGTACATGGCTAACATGGATTCAATTGCTTCGTTGGTTATGGCAGTTGTCGTCAATGACAGCGATGAAGATGGAGATAGCGCTGACGCAGTTCTCCCACAAAAGAAAAAGAGACTTTGGGGTTTGGTAGTTTGTCATAACACTACTCCAAGGTTTGTTCCTTTTCCTCTAAGGTATGCTTGTGAGTTTCTGGCTCAAGTGTTTGCCATCCATGTGAACAAAGAAATAGAGTTAGAATATCAGATTCTTGAGAAGAATATCCTGCGCACGCAGACGCTGTTGTGTGATATGTTGATGCGAGATGCACCCTTAGGTATTGTATCACAAAGCCCTAATATAATGGATCTAGTGAAATGTGATGGGGCTGCACTCTTTTATAGAAACAAGTTATGGTTATTAGGAGCGACACCGACTGAATCTCAATTAAGAGAGATAGCTTTATGGATGTCTGAGTATCATACAGATTCAACAGGTTTGAGTACAGACAGCTTGTCGGATGCAGGGTTTCCAGGGGCTCTTTCTCTTAGTGATACTGTATGTGGAATGGCAGCTGTTAGAATAACTTCAAAAGACATAGTTTTCTGGTTTAGGTCGCACACTGCTGCAGAAATCCGATGGGGTGGTGCAAAGCATGAACCGGGCGACCAAGACGATGGTAGGAAGATGCATCCAAGATCATCATTCAAGGCTTTCCTTGAAGTTGTGAAAGCCAGAAGCGTGCCGTGGAAAGACTTTGAAATGGATGCTATTCATTCGTTGCAGTTAATACTGAGAAATGCGTCCAAAGATACAGATATTATAGATTTGAATACGAAGGCAATCAATACAAGACTAAATGATTTGAAGATTGAAGGGATGCAGGAATTGGAAGCAGTGACAAGTGAGATGGTTAGATTAATTGAAACAGCAACAGTGCCTATTTTGGCAGTGGATGTTGATGGGACGGTCAACGGATGGAATATAAAAATCGCCGAGTTGACAGGTCTTCCGGTTGGCGAAGCTATTGGAAAACATCTACTCACCCTGGTTGAGGATTCTTCAACTGATATAGTCAAGAAGATGCTCAACTTGGCACTGCAGGGTATGCCTTTTTTCCTTTTCCATCTTATTATCCTTTACTTAAGACTCTAACTGTTGATTTTGTTCTCTTATAATGGCACTGCAGGGTGAAGAAGAGAAGAATGTTCAATTCGAGATAAAAACACATGGGGATCAGGTGGAATCCGGTCCTATTAGTTTGATAGTTAACGCATGTGCAAGCAAGGATCTTCGTGAAAATGTAGTGGGGGTTTGTTTTGTGGCCCAAGATATAACTGCTCAGAAGACTGTCATGGACAAATTCACCCGAATCGAAGGCGATTACAAAGCAATTGTGCAGAACCCAAATCAGTTGATCCCTCCTATATTCGGTACAGATGAATTTGGCTGGTGTTGTGAGTGGAATGCAGCTATGATTAAGTTAACCGGATGGAAGCGCGAGGAGGTGATGGATAAAATGCTTCTCGGAGAGGTTTTCGGAACTCAAATGTCTTGTTGTCGTCTAAAGAATCAAGAAGCTTTTGTTAATTTCGGCATTGTACTTAATAAAGCCATGACCGGTTTGGAAACAGAAAAGGTCCCTTTTGGCTTCTTCTCTCGAAAAGGCAAGTATGTAGAGTGCCTACTCTCGGTGAGTAAGAAAATCGACGCAGAGGGCCTAGTTACCGGAGTCTTCTGTTTCTTGCAGTTAGCTAGCCCTGAGCTGCAACAAGCATTACATATTCAGCGCCTGTCCGAACAAACCGCTCTCAAGAGACTGAAAGTACTAACTTACATGAAAAGGCAGATCAGGAATCCGTTGGCTGGGATCGTGTTTTCCAGTAAAATGCTGGAGGGTACTGACTTGGAAACCGAACAAAAGCGAATCGTGAACACTAGTTCTCAGTGCCAACGCCAGCTTAGCAAAATTCTTGACGACTCTGATCTCGACGGCATCATTGATGGGTACTTGGATCTTGAGATGGCTGAATTCACTTTCCAACGCCAGCTTAGCAAAATTCTTGACGACTCTGATCTCGACGGCATCATTGATGGGTACTTGGATCTTGAGATGGCTGAATTCACTTTACATGAGGTACTGGTTACCTCTCTTAGTCAAGTCATGAATAGGAGTAACACAAAGGGTATCCGTATAGCCAACGATGTTGCGGAGCATATCGCGAGGGAAACCTTGTATGGTGATAGTCTTAGGCTTCAGCAGGTCCTAGCTGACTTTTTACTAATTTCCATCAATTCCACACCTAATGGAGGCCAGGTTGTTATAGCAGCCTCCTTAACTAAAGAACAGTTGGGAAAATCTGTCCATCTTGTTAACTTGGAGCTCAGGTAACTGTTCCATATTCACGCATGTATCGATTTTCAGTATCCGATGATTTAAGCCTCGGTAATAATCTTAGCTTTGGTGAAACTGCTAACTGCAATAAACTANTATCTGTATATATTGTTCATTTGCGATTTGTGCTCAGCATAACACACGGTGGTAGTGGCGTGCCAGAAGCGGCGCTGAACCAGATGTTTGGAAATAATGTGCTAGAATCTGAGGAGGGTATTAGCCTACACATCAGTCGGAAGTTGTTAAAGCTTATGAATGGAGATGTTCGTTATTTAAAAGAAGCAGGAAAATCATCGTTTATTCTATCTGTTGAACTTGCAGCAGCTCATAAGTTGAAAGGTTGAAATTTTGGAAAAATAATAAAAGTGAACACGGCATGCTATTGTTCAATGTAACTCTTATTTTGTTTCATGTTTCTTTAACTTGTTGTAACGTATGTTGTAGATATGTGTATTAGTGTCTCTTTCACACTCGTGGCCTATTCTTATTTCTTAGTCATGCTTTATGTTTTTTGTTTTATTTTTAGAGCAATTAATGGCATGTGTGGGGATGAACGCAGTTGGGGAAAACAGTTAATAACAACTATGCTATGACTTACTGTACTAGTTTCTCAATATCTTCTTTCTCTGCTTTATTCAGTCCTGTCACCCATACTATATATAGTACACATATATAGTACAATCTAGGAAGAGTTTAAAGAGTACATTCCACCCAATTACAAGTTCTGAATAACAAACTAGTGACTCTTTTAACAATCTTGATTACTCACAGCACTGACAAATAAAAGCTTCCTACCCCAGATTTGAGCTACTTGTACCTCATTGCATTTCTCTTTGCCTCGCAATTGTGGAATGGACTCCTCCTCTTCTGACCCTATCCTTTCCAAATCTTCCTCTTCATAGTTCATGACATCCCGAGTCCATCAAAACTGACCTTGTCCTTAAGGTCAAAGGTCGGGTAAATAGCATATATGTCTCTCAAAACTTGGTTTTGATCATTAATAGTGTCTTTCTTCCAATCCATCACAGCTAACGGCTTGATGTGAGGCCGGTATCCAAGAGGCATATCCAAGGTTTTCATAGTTGTATCAAAATAGGGTTTCAAGTTTCAACTGTGATATATGGAACATAAGACGAATCCTATGGGTGC
TARGET=1110,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0695
PRIMER_LEFT_PENALTY=0.057411
PRIMER_RIGHT_PENALTY=0.012045
PRIMER_LEFT_SEQUENCE=ACCAAGTAGATGCTGTTGCTGA
PRIMER_RIGHT_SEQUENCE=ACCTAGATGGAGCCCTTAGTCC
PRIMER_LEFT=1070,22
PRIMER_RIGHT=1335,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.0810
PRIMER_LEFT_1_PENALTY=0.057411
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=ACCAAGTAGATGCTGTTGCTGA
PRIMER_RIGHT_1_SEQUENCE=GTGTGGGAGAAAGAAAGGAATG
PRIMER_LEFT_1=1070,22
PRIMER_RIGHT_1=1199,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=0.1438
PRIMER_LEFT_2_PENALTY=0.057411
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=ACCAAGTAGATGCTGTTGCTGA
PRIMER_RIGHT_2_SEQUENCE=TTAGTCCACAACTGGAAGCTCA
PRIMER_LEFT_2=1070,22
PRIMER_RIGHT_2=1320,22
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.1458
PRIMER_LEFT_3_PENALTY=0.122257
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=TTAGGAACAAAACCAAACCCAC
PRIMER_RIGHT_3_SEQUENCE=GTGTGGGAGAAAGAAAGGAATG
PRIMER_LEFT_3=979,22
PRIMER_RIGHT_3=1199,22
PRIMER_LEFT_3_TM=60.122
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.1661
PRIMER_LEFT_4_PENALTY=0.057411
PRIMER_RIGHT_4_PENALTY=0.108707
PRIMER_LEFT_4_SEQUENCE=ACCAAGTAGATGCTGTTGCTGA
PRIMER_RIGHT_4_SEQUENCE=CTCCTACCTAGATGGAGCCCTT
PRIMER_LEFT_4=1070,22
PRIMER_RIGHT_4=1340,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=60.109
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_03218 Ps_contig_mira-and-tgicl-ass_franssen_29211
SEQUENCE=GATATCATATACAAAAGTGAAGATTCAAATTCATGACATCCGACTTATTTATCTTTAAAAAATAAAATCTCGGCCACTAAACTACTTGATAAAAAAATTTATTATATCTATATAATTATATTTAAAAATGAGCGAATAGTGATAAAATTTGTCTAAACTATTATAAAAATAGTAAATATTAATTAAAATATTATAATTTTTAATTCTCAACTTATAAATATTAATAACTTATTTATTATAAATCATTTTTTACTTTTTATATATATTAAAAAATATAATAATTATGGTATAAAAATGAGTAAAATGTAATGGATTATGAGAAAATATATTATTTAAAAAGTAAAGTGATAATTGAATATGTGATTCTTACAAAATATGACTAACCAATAAAATAAGAAGAAACCTTAGAGGATAGGTAACCAAGTAAATATAATATAGTATAAATCTGGTAACACTAACAAGTGTTTCCCTGTGAGTCAGTAAGGTTACATGACAGTGGTACGTACGAGGCATGGGCTCATTTGTTAAATACAATTTCATTGTCAGACATGAGCTGTGATCTAAAAGATGTGAAATCGATAGAGAAAAACTCTCCACCAATTAAAATCAAACAATACGATGACATGGCATCACTATCATTCAGCCGCATCACGTAAAAACTTTCCGCATCCCTCGCACGTGACACCCATAACTCACTACTTTCCCATTATCCAACGCATGCCTCTTGGACACTCGTCCAATCAAGTTCCTCCACCTCACCCCTTTTTTTCCCCACCCCTATTCTATACTTAGCATGTTTAATTATTGAATTAGGATTCAATAATTATCCATTTATTAAATTAAATTAAAATTAATAGCCGACATGGAAAGTGAAGTTGAAAAAGAAAGTCATGTTTTCTCAGTAACAGTCATGTTTTCTGTTTAATTATGTGTCTGTTTTATCATTCATCATTTAACTGTCCTTTTTTTCTTATCTATTTTAGGAACAAAACCAAACCCACTTGCCCAAATTAGTTTTATTTTATTTTATTTTAAATTATAATATATAATAATAAATAAACAGCACCAACACCAAGTAGATGCTGTTGCTGAATGAATCTATCTAAGAGTTGGATTGGATTGGATGATGATTTGATTTTTTATTTTTATAATATTCTTTTATTTATCTTTATTCATTTCATTCCTTTCTTTCTCCCACACCTTCACATGGGAACACTATAAAATTCATATATCTTTATAGCATATGATGGCGGTCCCTTCTGTTCTCAGTCACAATTCTGTGTTGAATAATAATAATACTGAGCTTCCAGTTGTGGACTAAGGGCTCCATCTAGGTAGGAGACAAACAAGGCCGGTGATCCAGATTCACCATCTTATTTACTGTTGTTGCTGCTGCAGGTGCACGGTATGGTATGCTGGTCTCCCACTAATGGTTATTTTAACCCTTACCTTTAATACCTTTCTTTCTTCAACTTGTACTTGAACTTGAACTTTCACAACAGCCAAGTTTTTCCCTTTCAAGGTTAGTTAGTACTATTTTGCTTCTTGTGTTTATATTTCATCATGTGTCAGGGTTGTTGATTTGATTGTTTTCTATGATTGGGTTTTACTTCATAGATTGTGACTAGTTATTTTGGTAAAGCCTTTGATAATTTTGTCGTATTGATGATAGAGTTCAATAGTTTTGTAACATCATCGTATATGGTTTTGATTGACTTGGTTATCTTGTTTTTTTTACTGTTGTAAATGTAGTTTGTGTTAAAATGTCAACCACGAGGCCTAGCCAATCGTCCAACAATTCGGGGAGGTCAAGAAATAGTGCTAGGATTATTGCTCAGACGACTGTGGATGCAAAGCTTCATGCAACTTTTGAGGAGTCCGGTAGTTCGTTTGACTACTCGAGTTCGGTGCGTGTTTCTGGCTCGGTGGATGGAGATCAACAACCGAGGTCCAACAAAGTGACGACGGCTTACCTCAATCATATACAGAGAGGTAAGCAGATCCAGCCTTTCGGGTGCTTGCTAGCTTTAGATGAGAAAACGTGCAAGGTTGTTGCGTATAGTGAGAATGCGCCTGAGATGCTGACTATGGTGAGTCATGCTGTTCCTAGCGTTGGTGACCATCCTGCTCTTGGCATTGGAACTGACATAAGGACTGTTTTCACTGCGCCGAGTGCTTCTGCCTTGCAGAAGGCGCTAGGGTTTGCGGAGGTTTCACTTCTTAACCCGATTCTTGTTCATTGCAAGACTTCTGGGAAGCCGTTTTACGCGATCATTCATCGTGTTACTGGTAGTTTGATCATTGACTTTGAGCCGGTGAAGCCTTATGAAGTTCCCATGACTGCTGCGGGTGCCTTGCAATCTTACAAACTTGCTGCTAAAGCAATTACAAGATTGCAATCTTTGGCTAGTGGCAGCATGGAAAGGCTTTGTGATACCATGGTTCAAGAAGTTTTTGAACTAACGGGTTATGACAGGGTGATGGCTTATAAATTTCACGAGGATGATCACGGGGAGGTGATTGCTGAGATAGCAAAGCCAGGCCTAGAGCCATATCTAGGTCTGCACTATCCGGCGACAGATATTCCCCAGGCTGCGCGGTTTCTATTTATGAAGAACAAGGTCCGTATGATAGTTGATTGTAATGCAAAACATGTGAAGGTTCTTCAAGACGAAAAACTCCCATTTGATTTGACTCTGTGCGGTTCGACCTTGAGAGCTCCACATAGTTGCCATTTGCAGTACATGGCTAACATGGATTCAATTGCTTCGTTGGTTATGGCAGTTGTCGTCAATGACAGCGATGAAGATGGAGATAGCGCTGACGCAGTTCTCCCACAAAAGAAAAAGAGACTTTGGGGTTTGGTAGTTTGTCATAACACTACTCCAAGGTTTGTTCCTTTTCCTCTAAGGTATGCTTGTGAGTTTCTGGCTCAAGTGTTTGCCATCCATGTGAACAAAGAAATAGAGTTAGAATATCAGATTCTTGAGAAGAATATCCTGCGCACGCAGACGCTGTTGTGTGATATGTTGATGCGAGATGCACCCTTAGGTATTGTATCACAAAGCCCTAATATAATGGATCTAGTGAAATGTGATGGGGCTGCACTCTTTTATAGAAACAAGTTATGGTTATTAGGAGCGACACCGACTGAATCTCAATTAAGAGAGATAGCTTTATGGATGTCTGAGTATCATACAGATTCAACAGGTTTGAGTACAGACAGCTTGTCGGATGCAGGGTTTCCAGGGGCTCTTTCTCTTAGTGATACTGTATGTGGAATGGCAGCTGTTAGAATAACTTCAAAAGACATAGTTTTCTGGTTTAGGTCGCACACTGCTGCAGAAATCCGATGGGGTGGTGCAAAGCATGAACCGGGCGACCAAGACGATGGTAGGAAGATGCATCCAAGATCATCATTCAAGGCTTTCCTTGAAGTTGTGAAAGCCAGAAGCGTGCCGTGGAAAGACTTTGAAATGGATGCTATTCATTCGTTGCAGTTAATACTGAGAAATGCGTCCAAAGATACAGATATTATAGATTTGAATACGAAGGCAATCAATACAAGACTAAATGATTTGAAGATTGAAGGGATGCAGGAATTGGAAGCAGTGACAAGTGAGATGGTTAGATTAATTGAAACAGCAACAGTGCCTATTTTGGCAGTGGATGTTGATGGGACGGTCAACGGATGGAATATAAAAATCGCCGAGTTGACAGGTCTTCCGGTTGGCGAAGCTATTGGAAAACATCTACTCACCCTGGTTGAGGATTCTTCAACTGATATAGTCAAGAAGATGCTCAACTTGGCACTGCAGGGTATGCCTTTTTTCCTTTTCCATCTTATTATCCTTTACTTAAGACTCTAACTGTTGATTTTGTTCTCTTATAATGGCACTGCAGGGTGAAGAAGAGAAGAATGTTCAATTCGAGATAAAAACACATGGGGATCAGGTGGAATCCGGTCCTATTAGTTTGATAGTTAACGCATGTGCAAGCAAGGATCTTCGTGAAAATGTAGTGGGGGTTTGTTTTGTGGCCCAAGATATAACTGCTCAGAAGACTGTCATGGACAAATTCACCCGAATCGAAGGCGATTACAAAGCAATTGTGCAGAACCCAAATCAGTTGATCCCTCCTATATTCGGTACAGATGAATTTGGCTGGTGTTGTGAGTGGAATGCAGCTATGATTAAGTTAACCGGATGGAAGCGCGAGGAGGTGATGGATAAAATGCTTCTCGGAGAGGTTTTCGGAACTCAAATGTCTTGTTGTCGTCTAAAGAATCAAGAAGCTTTTGTTAATTTCGGCATTGTACTTAATAAAGCCATGACCGGTTTGGAAACAGAAAAGGTCCCTTTTGGCTTCTTCTCTCGAAAAGGCAAGTATGTAGAGTGCCTACTCTCGGTGAGTAAGAAAATCGACGCAGAGGGCCTAGTTACCGGAGTCTTCTGTTTCTTGCAGTTAGCTAGCCCTGAGCTGCAACAAGCATTACATATTCAGCGCCTGTCCGAACAAACCGCTCTCAAGAGACTGAAAGTACTAACTTACATGAAAAGGCAGATCAGGAATCCGTTGGCTGGGATCGTGTTTTCCAGTAAAATGCTGGAGGGTACTGACTTGGAAACCGAACAAAAGCGAATCGTGAACACTAGTTCTCAGTGCCAACGCCAGCTTAGCAAAATTCTTGACGACTCTGATCTCGACGGCATCATTGATGGGTACTTGGATCTTGAGATGGCTGAATTCACTTTCCAACGCCAGCTTAGCAAAATTCTTGACGACTCTGATCTCGACGGCATCATTGATGGGTACTTGGATCTTGAGATGGCTGAATTCACTTTACATGAGGTACTGGTTACCTCTCTTAGTCAAGTCATGAATAGGAGTAACACAAAGGGTATCCGTATAGCCAACGATGTTGCGGAGCATATCGCGAGGGAAACCTTGTATGGTGATAGTCTTAGGCTTCAGCAGGTCCTAGCTGACTTTTTACTAATTTCCATCAATTCCACACCTAATGGAGGCCAGGTTGTTATAGCAGCCTCCTTAACTAAAGAACAGTTGGGAAAATCTGTCCATCTTGTTAACTTGGAGCTCAGGTAACTGTTCCATATTCACGCATGTATCGATTTTCAGTATCCGATGATTTAAGCCTCGGTAATAATCTTAGCTTTGGTGAAACTGCTAACTGCAATAAACTANTATCTGTATATATTGTTCATTTGCGATTTGTGCTCAGCATAACACACGGTGGTAGTGGCGTGCCAGAAGCGGCGCTGAACCAGATGTTTGGAAATAATGTGCTAGAATCTGAGGAGGGTATTAGCCTACACATCAGTCGGAAGTTGTTAAAGCTTATGAATGGAGATGTTCGTTATTTAAAAGAAGCAGGAAAATCATCGTTTATTCTATCTGTTGAACTTGCAGCAGCTCATAAGTTGAAAGGTTGAAATTTTGGAAAAATAATAAAAGTGAACACGGCATGCTATTGTTCAATGTAACTCTTATTTTGTTTCATGTTTCTTTAACTTGTTGTAACGTATGTTGTAGATATGTGTATTAGTGTCTCTTTCACACTCGTGGCCTATTCTTATTTCTTAGTCATGCTTTATGTTTTTTGTTTTATTTTTAGAGCAATTAATGGCATGTGTGGGGATGAACGCAGTTGGGGAAAACAGTTAATAACAACTATGCTATGACTTACTGTACTAGTTTCTCAATATCTTCTTTCTCTGCTTTATTCAGTCCTGTCACCCATACTATATATAGTACACATATATAGTACAATCTAGGAAGAGTTTAAAGAGTACATTCCACCCAATTACAAGTTCTGAATAACAAACTAGTGACTCTTTTAACAATCTTGATTACTCACAGCACTGACAAATAAAAGCTTCCTACCCCAGATTTGAGCTACTTGTACCTCATTGCATTTCTCTTTGCCTCGCAATTGTGGAATGGACTCCTCCTCTTCTGACCCTATCCTTTCCAAATCTTCCTCTTCATAGTTCATGACATCCCGAGTCCATCAAAACTGACCTTGTCCTTAAGGTCAAAGGTCGGGTAAATAGCATATATGTCTCTCAAAACTTGGTTTTGATCATTAATAGTGTCTTTCTTCCAATCCATCACAGCTAACGGCTTGATGTGAGGCCGGTATCCAAGAGGCATATCCAAGGTTTTCATAGTTGTATCAAAATAGGGTTTCAAGTTTCAACTGTGATATATGGAACATAAGACGAATCCTATGGGTGC
TARGET=1286,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0356
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.012045
PRIMER_LEFT_SEQUENCE=CATTCCTTTCTTTCTCCCACAC
PRIMER_RIGHT_SEQUENCE=ACCTAGATGGAGCCCTTAGTCC
PRIMER_LEFT=1178,22
PRIMER_RIGHT=1335,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=0.0363
PRIMER_LEFT_1_PENALTY=0.024206
PRIMER_RIGHT_1_PENALTY=0.012045
PRIMER_LEFT_1_SEQUENCE=GAGTTGGATTGGATTGGATGAT
PRIMER_RIGHT_1_SEQUENCE=ACCTAGATGGAGCCCTTAGTCC
PRIMER_LEFT_1=1106,22
PRIMER_RIGHT_1=1335,22
PRIMER_LEFT_1_TM=60.024
PRIMER_RIGHT_1_TM=59.988
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.0695
PRIMER_LEFT_2_PENALTY=0.057411
PRIMER_RIGHT_2_PENALTY=0.012045
PRIMER_LEFT_2_SEQUENCE=ACCAAGTAGATGCTGTTGCTGA
PRIMER_RIGHT_2_SEQUENCE=ACCTAGATGGAGCCCTTAGTCC
PRIMER_LEFT_2=1070,22
PRIMER_RIGHT_2=1335,22
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=59.988
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.1099
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.086339
PRIMER_LEFT_3_SEQUENCE=CATTCCTTTCTTTCTCCCACAC
PRIMER_RIGHT_3_SEQUENCE=TTAGTCCACAACTGGAAGCTCA
PRIMER_LEFT_3=1178,22
PRIMER_RIGHT_3=1320,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=0.1105
PRIMER_LEFT_4_PENALTY=0.024206
PRIMER_RIGHT_4_PENALTY=0.086339
PRIMER_LEFT_4_SEQUENCE=GAGTTGGATTGGATTGGATGAT
PRIMER_RIGHT_4_SEQUENCE=TTAGTCCACAACTGGAAGCTCA
PRIMER_LEFT_4=1106,22
PRIMER_RIGHT_4=1320,22
PRIMER_LEFT_4_TM=60.024
PRIMER_RIGHT_4_TM=59.914
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_03219 Ps_contig_mira-and-tgicl-ass_franssen_29215
SEQUENCE=TCGGGGGGTTCTTAGTCACGTTTCTTCAATGTGGACTCTGTACTAAGCATCTCACTGAGAGAAAGAGAGAGAGGAGAGAGAGAGAGAATGTCTTCCCAAGCAAACGCCGAGACATGGATCTGATGAAGCTGATGATGGCTGATTACAAAGGTGGAGTATTATACAATGATACCATGACAAGAGCTTCTTTGGTACGTAATTTCCGAACCGG
TARGET=64,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2661
PRIMER_LEFT_PENALTY=0.225996
PRIMER_RIGHT_PENALTY=0.040146
PRIMER_LEFT_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_SEQUENCE=CCTTTGTAATCAGCCATCATCA
PRIMER_LEFT=18,22
PRIMER_RIGHT=150,22
PRIMER_LEFT_TM=59.774
PRIMER_RIGHT_TM=59.960
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=0.3288
PRIMER_LEFT_1_PENALTY=0.225996
PRIMER_RIGHT_1_PENALTY=0.102789
PRIMER_LEFT_1_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_1_SEQUENCE=TTTGTAATCAGCCATCATCAGC
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=148,22
PRIMER_LEFT_1_TM=59.774
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.4014
PRIMER_LEFT_2_PENALTY=0.361206
PRIMER_RIGHT_2_PENALTY=0.040146
PRIMER_LEFT_2_SEQUENCE=TCACGTTTCTTCAATGTGGACT
PRIMER_RIGHT_2_SEQUENCE=CCTTTGTAATCAGCCATCATCA
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=150,22
PRIMER_LEFT_2_TM=59.639
PRIMER_RIGHT_2_TM=59.960
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.4014
PRIMER_LEFT_3_PENALTY=0.361206
PRIMER_RIGHT_3_PENALTY=0.040146
PRIMER_LEFT_3_SEQUENCE=CACGTTTCTTCAATGTGGACTC
PRIMER_RIGHT_3_SEQUENCE=CCTTTGTAATCAGCCATCATCA
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=150,22
PRIMER_LEFT_3_TM=59.639
PRIMER_RIGHT_3_TM=59.960
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=0.4014
PRIMER_LEFT_4_PENALTY=0.361206
PRIMER_RIGHT_4_PENALTY=0.040146
PRIMER_LEFT_4_SEQUENCE=AGTCACGTTTCTTCAATGTGGA
PRIMER_RIGHT_4_SEQUENCE=CCTTTGTAATCAGCCATCATCA
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=150,22
PRIMER_LEFT_4_TM=59.639
PRIMER_RIGHT_4_TM=59.960
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_03220 Ps_contig_mira-and-tgicl-ass_franssen_29215
SEQUENCE=TCGGGGGGTTCTTAGTCACGTTTCTTCAATGTGGACTCTGTACTAAGCATCTCACTGAGAGAAAGAGAGAGAGGAGAGAGAGAGAGAATGTCTTCCCAAGCAAACGCCGAGACATGGATCTGATGAAGCTGATGATGGCTGATTACAAAGGTGGAGTATTATACAATGATACCATGACAAGAGCTTCTTTGGTACGTAATTTCCGAACCGG
TARGET=74,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2661
PRIMER_LEFT_PENALTY=0.225996
PRIMER_RIGHT_PENALTY=0.040146
PRIMER_LEFT_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_SEQUENCE=CCTTTGTAATCAGCCATCATCA
PRIMER_LEFT=18,22
PRIMER_RIGHT=150,22
PRIMER_LEFT_TM=59.774
PRIMER_RIGHT_TM=59.960
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=0.3288
PRIMER_LEFT_1_PENALTY=0.225996
PRIMER_RIGHT_1_PENALTY=0.102789
PRIMER_LEFT_1_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_1_SEQUENCE=TTTGTAATCAGCCATCATCAGC
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=148,22
PRIMER_LEFT_1_TM=59.774
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.4014
PRIMER_LEFT_2_PENALTY=0.361206
PRIMER_RIGHT_2_PENALTY=0.040146
PRIMER_LEFT_2_SEQUENCE=TCACGTTTCTTCAATGTGGACT
PRIMER_RIGHT_2_SEQUENCE=CCTTTGTAATCAGCCATCATCA
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=150,22
PRIMER_LEFT_2_TM=59.639
PRIMER_RIGHT_2_TM=59.960
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.4014
PRIMER_LEFT_3_PENALTY=0.361206
PRIMER_RIGHT_3_PENALTY=0.040146
PRIMER_LEFT_3_SEQUENCE=CACGTTTCTTCAATGTGGACTC
PRIMER_RIGHT_3_SEQUENCE=CCTTTGTAATCAGCCATCATCA
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=150,22
PRIMER_LEFT_3_TM=59.639
PRIMER_RIGHT_3_TM=59.960
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=0.4014
PRIMER_LEFT_4_PENALTY=0.361206
PRIMER_RIGHT_4_PENALTY=0.040146
PRIMER_LEFT_4_SEQUENCE=AGTCACGTTTCTTCAATGTGGA
PRIMER_RIGHT_4_SEQUENCE=CCTTTGTAATCAGCCATCATCA
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=150,22
PRIMER_LEFT_4_TM=59.639
PRIMER_RIGHT_4_TM=59.960
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_03221 Ps_contig_mira-and-tgicl-ass_franssen_29216
SEQUENCE=AAAAGAACAAAGTAGGTAATCAACGCAGAGTACGTCGGGGGGTCTTAGTCACGTTTCTTCAATGTGGACTCTGTACTAAGCATCTCACTGAGAGAAAGAGAGAGAGGAGAGAGAGAGAGAATGTCTTCCCCAAGCAAACGCCGAGACATGGATCTGATGAAGCTGATGATGGCTGATTACAAAGTGGAGATTATCAATGATACCATGCAAGAGCTTCTTTGTCGTAATTTCACGGACCTAAAGACAGTCCTTATCATGAAGGTGTGTGGAAGATTAGAGTGGAGTTACCTGATGCTTATCCTTATAAGTCTCCTTCTATTGGTTTTGCCAACAAAATTTACCATCCTAATGTTGACGAAATGTCTGGGGCAGTTTGCTTAGATGTTATTAACCAGACTTGGAGTCCAATGTTTGATCTTGTTAATGTGTTCGAAGTGTTTCTTCCTCAACTTCTTATGTACCCCAATGCAGCAGATCCATTGAATAGTGATGCCGCTGCCTTGATGATGCAAGACCGTGCTGCATATGAACTAAAAGTCAAAGAATACTGTGAAAAATATGCCAAGCCAGAAGATATTGGAGCTTCTAAGGAGGAAGATTCTAGTGATGAAGAGCTGAGTGAAGATGATGATTATGCCTCGAGTGATGACGGCATTGCTGGCCAACCTGATCCCTAAATTGTTATACATACAATGTCTGCATTATTATATTCATGCTTGTACATTACTACATGATATGATATTGAAGTACTTGAATTAAGAAGAAACAATTTCTGNAAGGTTAGGAAAGGAAAAATACCCTCCTACCCTTTTCCTTA
TARGET=97,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2683
PRIMER_LEFT_PENALTY=0.165486
PRIMER_RIGHT_PENALTY=0.102789
PRIMER_LEFT_SEQUENCE=GTAATCAACGCAGAGTACGTCG
PRIMER_RIGHT_SEQUENCE=TTTGTAATCAGCCATCATCAGC
PRIMER_LEFT=15,22
PRIMER_RIGHT=182,22
PRIMER_LEFT_TM=59.835
PRIMER_RIGHT_TM=60.103
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.3288
PRIMER_LEFT_1_PENALTY=0.225996
PRIMER_RIGHT_1_PENALTY=0.102789
PRIMER_LEFT_1_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_1_SEQUENCE=TTTGTAATCAGCCATCATCAGC
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=182,22
PRIMER_LEFT_1_TM=59.774
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=0.3325
PRIMER_LEFT_2_PENALTY=0.165486
PRIMER_RIGHT_2_PENALTY=0.167063
PRIMER_LEFT_2_SEQUENCE=GTAATCAACGCAGAGTACGTCG
PRIMER_RIGHT_2_SEQUENCE=AGGACTGTCTTTAGGTCCGTGA
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=250,22
PRIMER_LEFT_2_TM=59.835
PRIMER_RIGHT_2_TM=60.167
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.3916
PRIMER_LEFT_3_PENALTY=0.165486
PRIMER_RIGHT_3_PENALTY=0.226143
PRIMER_LEFT_3_SEQUENCE=GTAATCAACGCAGAGTACGTCG
PRIMER_RIGHT_3_SEQUENCE=TCAGCTTCATCAGATCCATGTC
PRIMER_LEFT_3=15,22
PRIMER_RIGHT_3=165,22
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=60.226
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.1000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.3931
PRIMER_LEFT_4_PENALTY=0.225996
PRIMER_RIGHT_4_PENALTY=0.167063
PRIMER_LEFT_4_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_4_SEQUENCE=AGGACTGTCTTTAGGTCCGTGA
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=250,22
PRIMER_LEFT_4_TM=59.774
PRIMER_RIGHT_4_TM=60.167
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_03222 Ps_contig_mira-and-tgicl-ass_franssen_29216
SEQUENCE=AAAAGAACAAAGTAGGTAATCAACGCAGAGTACGTCGGGGGGTCTTAGTCACGTTTCTTCAATGTGGACTCTGTACTAAGCATCTCACTGAGAGAAAGAGAGAGAGGAGAGAGAGAGAGAATGTCTTCCCCAAGCAAACGCCGAGACATGGATCTGATGAAGCTGATGATGGCTGATTACAAAGTGGAGATTATCAATGATACCATGCAAGAGCTTCTTTGTCGTAATTTCACGGACCTAAAGACAGTCCTTATCATGAAGGTGTGTGGAAGATTAGAGTGGAGTTACCTGATGCTTATCCTTATAAGTCTCCTTCTATTGGTTTTGCCAACAAAATTTACCATCCTAATGTTGACGAAATGTCTGGGGCAGTTTGCTTAGATGTTATTAACCAGACTTGGAGTCCAATGTTTGATCTTGTTAATGTGTTCGAAGTGTTTCTTCCTCAACTTCTTATGTACCCCAATGCAGCAGATCCATTGAATAGTGATGCCGCTGCCTTGATGATGCAAGACCGTGCTGCATATGAACTAAAAGTCAAAGAATACTGTGAAAAATATGCCAAGCCAGAAGATATTGGAGCTTCTAAGGAGGAAGATTCTAGTGATGAAGAGCTGAGTGAAGATGATGATTATGCCTCGAGTGATGACGGCATTGCTGGCCAACCTGATCCCTAAATTGTTATACATACAATGTCTGCATTATTATATTCATGCTTGTACATTACTACATGATATGATATTGAAGTACTTGAATTAAGAAGAAACAATTTCTGNAAGGTTAGGAAAGGAAAAATACCCTCCTACCCTTTTCCTTA
TARGET=107,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2683
PRIMER_LEFT_PENALTY=0.165486
PRIMER_RIGHT_PENALTY=0.102789
PRIMER_LEFT_SEQUENCE=GTAATCAACGCAGAGTACGTCG
PRIMER_RIGHT_SEQUENCE=TTTGTAATCAGCCATCATCAGC
PRIMER_LEFT=15,22
PRIMER_RIGHT=182,22
PRIMER_LEFT_TM=59.835
PRIMER_RIGHT_TM=60.103
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.3288
PRIMER_LEFT_1_PENALTY=0.225996
PRIMER_RIGHT_1_PENALTY=0.102789
PRIMER_LEFT_1_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_1_SEQUENCE=TTTGTAATCAGCCATCATCAGC
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=182,22
PRIMER_LEFT_1_TM=59.774
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=0.3325
PRIMER_LEFT_2_PENALTY=0.165486
PRIMER_RIGHT_2_PENALTY=0.167063
PRIMER_LEFT_2_SEQUENCE=GTAATCAACGCAGAGTACGTCG
PRIMER_RIGHT_2_SEQUENCE=AGGACTGTCTTTAGGTCCGTGA
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=250,22
PRIMER_LEFT_2_TM=59.835
PRIMER_RIGHT_2_TM=60.167
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.3916
PRIMER_LEFT_3_PENALTY=0.165486
PRIMER_RIGHT_3_PENALTY=0.226143
PRIMER_LEFT_3_SEQUENCE=GTAATCAACGCAGAGTACGTCG
PRIMER_RIGHT_3_SEQUENCE=TCAGCTTCATCAGATCCATGTC
PRIMER_LEFT_3=15,22
PRIMER_RIGHT_3=165,22
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=60.226
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.1000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.3931
PRIMER_LEFT_4_PENALTY=0.225996
PRIMER_RIGHT_4_PENALTY=0.167063
PRIMER_LEFT_4_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_4_SEQUENCE=AGGACTGTCTTTAGGTCCGTGA
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=250,22
PRIMER_LEFT_4_TM=59.774
PRIMER_RIGHT_4_TM=60.167
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_03223 Ps_contig_mira-and-tgicl-ass_franssen_29217
SEQUENCE=GTCGGGGGGTCTTAGTCACGTTTCTTCAATGTGGACTCTGTACTAAGCATCTCACTGAGAGAAAGAGAGAGAGGAGAGAGAGAGAGAATGTCTTCCCAAGCAAACGCCGAGACATGGATCTGATGAAGCTGATGATGGCTGATTACAAGTGGAGTATTATCAATGATACCATGACAAGAGCCTTTCGTTTTGGTACGTAATTTTCCGAACCGGTAACCTAA
TARGET=64,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2824
PRIMER_LEFT_PENALTY=0.225996
PRIMER_RIGHT_PENALTY=0.056364
PRIMER_LEFT_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_SEQUENCE=TTACGTACCAAAACGAAAGGCT
PRIMER_LEFT=18,22
PRIMER_RIGHT=199,22
PRIMER_LEFT_TM=59.774
PRIMER_RIGHT_TM=60.056
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=0.3344
PRIMER_LEFT_1_PENALTY=0.225996
PRIMER_RIGHT_1_PENALTY=0.108385
PRIMER_LEFT_1_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_1_SEQUENCE=GGTTCGGAAAATTACGTACCAA
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=210,22
PRIMER_LEFT_1_TM=59.774
PRIMER_RIGHT_1_TM=60.108
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.3583
PRIMER_LEFT_2_PENALTY=0.225996
PRIMER_RIGHT_2_PENALTY=0.132262
PRIMER_LEFT_2_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_2_SEQUENCE=CTCCACTTGTAATCAGCCATCA
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=153,22
PRIMER_LEFT_2_TM=59.774
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.3882
PRIMER_LEFT_3_PENALTY=0.225996
PRIMER_RIGHT_3_PENALTY=0.162217
PRIMER_LEFT_3_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_3_SEQUENCE=GTACCAAAACGAAAGGCTCTTG
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=59.774
PRIMER_RIGHT_3_TM=60.162
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=178
PRIMER_PAIR_PENALTY_4=0.4176
PRIMER_LEFT_4_PENALTY=0.361206
PRIMER_RIGHT_4_PENALTY=0.056364
PRIMER_LEFT_4_SEQUENCE=TCACGTTTCTTCAATGTGGACT
PRIMER_RIGHT_4_SEQUENCE=TTACGTACCAAAACGAAAGGCT
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=59.639
PRIMER_RIGHT_4_TM=60.056
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_03224 Ps_contig_mira-and-tgicl-ass_franssen_29217
SEQUENCE=GTCGGGGGGTCTTAGTCACGTTTCTTCAATGTGGACTCTGTACTAAGCATCTCACTGAGAGAAAGAGAGAGAGGAGAGAGAGAGAGAATGTCTTCCCAAGCAAACGCCGAGACATGGATCTGATGAAGCTGATGATGGCTGATTACAAGTGGAGTATTATCAATGATACCATGACAAGAGCCTTTCGTTTTGGTACGTAATTTTCCGAACCGGTAACCTAA
TARGET=74,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2824
PRIMER_LEFT_PENALTY=0.225996
PRIMER_RIGHT_PENALTY=0.056364
PRIMER_LEFT_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_SEQUENCE=TTACGTACCAAAACGAAAGGCT
PRIMER_LEFT=18,22
PRIMER_RIGHT=199,22
PRIMER_LEFT_TM=59.774
PRIMER_RIGHT_TM=60.056
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=0.3344
PRIMER_LEFT_1_PENALTY=0.225996
PRIMER_RIGHT_1_PENALTY=0.108385
PRIMER_LEFT_1_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_1_SEQUENCE=GGTTCGGAAAATTACGTACCAA
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=210,22
PRIMER_LEFT_1_TM=59.774
PRIMER_RIGHT_1_TM=60.108
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.3583
PRIMER_LEFT_2_PENALTY=0.225996
PRIMER_RIGHT_2_PENALTY=0.132262
PRIMER_LEFT_2_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_2_SEQUENCE=CTCCACTTGTAATCAGCCATCA
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=153,22
PRIMER_LEFT_2_TM=59.774
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.3882
PRIMER_LEFT_3_PENALTY=0.225996
PRIMER_RIGHT_3_PENALTY=0.162217
PRIMER_LEFT_3_SEQUENCE=CGTTTCTTCAATGTGGACTCTG
PRIMER_RIGHT_3_SEQUENCE=GTACCAAAACGAAAGGCTCTTG
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=59.774
PRIMER_RIGHT_3_TM=60.162
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=178
PRIMER_PAIR_PENALTY_4=0.4176
PRIMER_LEFT_4_PENALTY=0.361206
PRIMER_RIGHT_4_PENALTY=0.056364
PRIMER_LEFT_4_SEQUENCE=TCACGTTTCTTCAATGTGGACT
PRIMER_RIGHT_4_SEQUENCE=TTACGTACCAAAACGAAAGGCT
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=59.639
PRIMER_RIGHT_4_TM=60.056
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_03225 Ps_contig_mira-and-tgicl-ass_franssen_29224
SEQUENCE=TCGGGGCNCGCTCACTCACTCACCCTCCACTACCAACTACCAACTCATATATACCACCAACACCACCCTCCAATCGAAACAACCATCGCAAAAATGGCCATGGCTCTTCGCAGACTCACCTCCACCATCAACAAACAAACTTCACCTTTCTCCAACACCGCTTCTCTCCGTCGCATGTCATCTTCGCTCTCCGCACAAGTGAATCACAAATCTAGCCCCGATTGGATTAAACAGCTCAACGATCCTCTTGAGGTGGTGGATCCTGAGATTGATAATATAATCGAACTTGAAAAAGCTCGTCAATGGAAGGGACTAGAACTTATACCTTCAGAAAATTTTACGTCCTTGTCGGTGATGCAAGCAGTTGGATCTGTTATGACGAATAAATACAGTGAAGGTTATCCTGGTGCTAGATACTATGGTGGAAATGAGTACATTGACATGGCTGAGACCTTGTGTCAGAAGCGGGCACTGGAAACTTTTGGGTTGGATCCAACACTATGGGGAGTCAATGTGCAGTCGCTATCGGGATCTCCTTCCAATTTCCAAGTTTACACTGCTTTATTGAAACCTCATGAGAGAATTATGGCACTCGATCTTCCCCATGGTGGGCATCTTTCACATGGATATCAGACTGACACCAAGAAGATATCAGCTGTATCTATATTCTTTGAAACAATGCCGTACAGATTGGACGAGAGCACTGGTTATATTGATTATGACCAGATGGAGAAAAGTGCTGCACTATTTAGGCCAAAATTAATAGTTGCTGGTGCTAGTGCTTATGCCCGCCTTTATGATTATGCACGTATTCGCAAGGTCTGTGATAAACAGAAGGCAGTTATGTTAGCTGATATGGCACACATCAGTGGATTAGTTGCTGCAGGTGTTATTCCTTCTCCTTTTGATTATGCAGATGTTGTAACAACCACAACACATAAGTCACTTCGTGGGCCACGTGGGGCTATGATATTCTTCAGGAAGGGTGTGAAAGAGATAAACAAGAAAGGGCAGGAAGTGCTCTATGACTATGAAGACAAAATAAATCAGGCTGTTTTTCCTGGACTTCAAGGTGGTCCTCACAATCACACTATTACAGGCCTAGCAGTTGCATTAAAGCAGGCTATGACACCAGAGTTCAAGAGTTATCAGAAACAAGTTCTTAGTAACTCCTCGACTTTTGCACAGAGTTTGATAGAGAGAGGCTATGACCTTGTATCTGGCGGAACTGAGAACCATTTGGTGTTAGTGAACTTAAGAGACAAGGGCATTGATGGCTCCAGGGTTGAGAAGGTGTTGGAATCAGTTCATATAGCTGCCAATAAAAATACTGTTCCAGGGGATGTGTCTGCTATGGTTCCAGGGGGTATCAGAATGGGAACCCCTGCTCTCACATCCAGGGGTTTTGTTGAGGGTGATTTTGAGAAAGTAGCTGAATACTTTGACGCTGCAGTCAAAATAGCCTTACAGATCAAAGAAAATTCTAAAGGCACAAAGTTGAAGGATTTTGTGGAAGCTATGCAATCAGATGCACAAATTCAATCTCAAATTGCTAATCTCCGACGTGAAGTAGAAGATTATGCTAAGCAGTTTCCCACAATCGGTTTTGAGAAAGAGACAATGAAGTATAATAAGTGAAGATGAAGTATGGAGTTTCTTTAAGAGTTTATGGAATAACAACCCAAATTTGGATATGTTGCTCAACATATTCTAACTCTGGTCTTAGAAGTTGTGAATTATATTATCAAAGGTTAAGTGAAAAAGAAAAGGGAAAAGTTTTATGACAATGATAATAATCCTTTTATATAGGGATGAGCATTTTCTTTCAAACTTGATATAGATAATTATTTGGAGTTGAAAGTTCCTTTTGATGATGAAGTTTGAGCATTTTTGGATTTGTTATTTTCTTATTTGTATTATTATTACCTTTGAAGAAATAGACTGCCCCTTAAAATTCCTTGTGGCATTACAATTATTAAGAAAAAAACAAAGTGATT
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03226 Ps_contig_mira-and-tgicl-ass_franssen_29226
SEQUENCE=GGTATCAACGCAGAGTACGGGGGAGAACTTATGNGTTCCATTTCATCCATTCTCAGCCTCAAGAGGGCAGTGGCAGCTCTTACTTCTGCCATATTTCACCAAACTCTCTGAAATCCTCCCTGGTGATTCTTTGGTGCTAAAAGAAAGGCAAGAGTTGTTTGAGTTCAAAGAGGTAGCAATTTCAAGCTAAGGGAAGATGGACTATGTTAATGGACCAGGAAGAAACCATCTCTTTGTTCCAGGGCCGGTTAACATACCTGACCAGGTCATTCGGGCTATGAGCCGAAACAACGAGGACTATCGTTCTCCTGCAATTCCAGCTCTCACAAAAGAGCTACTTGAGGATGTCAAGAAGATTTTCAAGACTACTACCGGAACACCCTTTCTTTTCCCTACCACTGGTACTGGTGCTTGGGAGAGTGCTCTGACAAACACATTGTCGCCTGGAGATCGAATTGTATCTTTTGTGATAGGCCAGTTCAGTTTGCTTTGGATTGACCAGCAGCAGCGCCTAAAATATAACGTCGATGTTGTGGAAAGTGAATGGGGCCGTGGTGCTGATCTCGACGTTTTGGAGTCAAAACTTGCTTCAGATTCTGCACACACTATTAAGGCTATTTGCATTGTTCACAATGAGACTGCAACTGGTGTCACCAATAACTTGGCCAAAGTCCGACAACTTCTTGATGCATACAGCCATCCAGCTCTTCTTCTTGTTGATGGAGTGTCATCCATTTGTGCTCTCGATTTCCGTATGGACGAATGGGGAGTAGATGTGGCCATAACTGGTTCTCAGAAAGCCCTTTCTCTTCCTACTGGGATAGGATTTGTGGTTGCAAGTCCTAAAGCTATTGAAGCTTCGAAAACTGCTAAGTCGCTTCGAGTTTTCTTTGATTGGAGTGACTACCTGAAATTCTACAAGTTAGGAACCTATTGGCCATACACTCCTTCCATTCAGCTTCTCTATGGTCTCAGGGCAGCTCTTGATCTCATTTTCGAGGAAGGACTTGAAAATATTTTTGCAAGGCACAATCGTTTAGGTACAGCAACCAGACTTGCTGTGGAGGCATGGGGATTGAAGAATTGCACACAAAAGGAAGAGTGGTTCAGTGACACTGTGACTGCTGTTCTTGTTCCTCCAAACATTGACGGTGCTGAAATTGTAAGAAGGGCATGGAAAAGATACAATCTGAGCTTAGGTTTGGGACTAAACAAAGTTGCTGGCAAGGTTTTCAGAATTGGACACCTTGGTAACATGAATGAGTTGCAATTATTGGGATGTCTTGCTGGTGTGGAGATGATTCTGAAAGATGTGGGCTATCCAGTGAAGCTAGGAAGTGGAGTTGCTGCTGCCAGTGCATACTTACAGAACAATATTCCTCTCATCCCTTCAAGGATTTAAGTTACATGCTCATTCTTGTAATTACTTCCATTTATGCTCTTTCAAGATATATGTATAATACAACATAATGTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTGTTATTGTTCCCTTTTGTAACTACTTTTAGAAGATGAACATATTTGCTACTTTTAGTT
TARGET=1473,48
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03227 Ps_contig_mira-and-tgicl-ass_franssen_29228
SEQUENCE=GAGTTATCACAAAAAAATCACACATAGTACTAAACGAGACGTGGTCACATTATTTGCCNGTCATTGAGTATTTCTTACATACATCACCACATCCACACACACCTTATTAAAGATAATATTTCCATTCTCCTATGTTTCAGTTAAACTCTACCATACCACACACACAACACAAGGCAAAGGTAACATAGCAATGGCTCAAACTATGTTGCTCATGTCTAGTGTCTCCAGTACCTATTCTGTTCCCTTGAACAAAGATCCTTTGCTTCAATTACAGTGTCAAAGATTGAAGCCTAGGTTTTCTGATATCTCATTTAGCCCACTTTCTTCTAATTCTAAGTCTTTTTCATCTCGCACTTTCAAAACTCTGGCTCTATTCAAATCAAAGACCAAAGCCCCTGCTAAGGTTGTACCAAAACAAAAACCAAAGGTTGAAGATGGTGTCTTTGGAACTTCTGGAGGATTTGGTTTCACTAAGCAGAACGAACTCTTTGTTGGTCGTGTTGCTATGATCGGTTTTGCTGCATCAATTTTGGGTGAAGGAATAACTGGGAAAGGAATTCTATCACAATTGAATTTGGAAACTGGAATTCCAATTTATGAAGCTGAGCCTCTCCTCCTATTCTTCATCATTTTTACACTTCTAGGAGCCATTGGAGCTCTAGGTGACCGTGGTAAATTCGTCGACGATGAACCTACCACCGGAGGCGTTATTCCACCCGGAAAAGGCTTCAGACAAGCTCTCGGTCTCAGCGAAGGAGGTCCTTTATTTGGATTCACCAAATCCAATGAACTGTTTGTTGGAAGATTGGCTCAACTGGGATTTGCTTTCTCTTTGATTGGAGAGATTATTACCGGGAAGGGAGCACTAGCTCAACTCAACATTGAAACTGGTGTACCAATCAATGAGATTGAGCCACTTGTGTTGTTCAATGTTGCTTTCTTCTTCATTGCTGCTTTGAATCCTGGAACTGGGAAATTTGTAACAGATGAGGAAGAAGATTAGATTAGAGAGTAGTTTACTTTGTTTGTATTTAAAGAAAACTTGTACGTTTGTTTGTCTATGATCAATGCATTGAGTTCTTGGTAAACAGCTATATTATATCATCTACGTATTTGTTCAATACTTGCAACTGTGTATTAACCAATGAAACAATTCAT
TARGET=157,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2206
PRIMER_LEFT_PENALTY=0.159749
PRIMER_RIGHT_PENALTY=0.060824
PRIMER_LEFT_SEQUENCE=CATACATCACCACATCCACACA
PRIMER_RIGHT_SEQUENCE=AGAGCCAGAGTTTTGAAAGTGC
PRIMER_LEFT=77,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=60.160
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=0.3852
PRIMER_LEFT_1_PENALTY=0.159749
PRIMER_RIGHT_1_PENALTY=0.225403
PRIMER_LEFT_1_SEQUENCE=CATACATCACCACATCCACACA
PRIMER_RIGHT_1_SEQUENCE=CATGAGCAACATAGTTTGAGCC
PRIMER_LEFT_1=77,22
PRIMER_RIGHT_1=213,22
PRIMER_LEFT_1_TM=60.160
PRIMER_RIGHT_1_TM=59.775
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=0.3852
PRIMER_LEFT_2_PENALTY=0.159749
PRIMER_RIGHT_2_PENALTY=0.225403
PRIMER_LEFT_2_SEQUENCE=CATACATCACCACATCCACACA
PRIMER_RIGHT_2_SEQUENCE=ATGAGCAACATAGTTTGAGCCA
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=60.160
PRIMER_RIGHT_2_TM=59.775
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.3852
PRIMER_LEFT_3_PENALTY=0.159749
PRIMER_RIGHT_3_PENALTY=0.225403
PRIMER_LEFT_3_SEQUENCE=CATACATCACCACATCCACACA
PRIMER_RIGHT_3_SEQUENCE=TGAGCAACATAGTTTGAGCCAT
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=60.160
PRIMER_RIGHT_3_TM=59.775
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=0.4220
PRIMER_LEFT_4_PENALTY=0.361206
PRIMER_RIGHT_4_PENALTY=0.060824
PRIMER_LEFT_4_SEQUENCE=CCACATCCACACACACCTTATT
PRIMER_RIGHT_4_SEQUENCE=AGAGCCAGAGTTTTGAAAGTGC
PRIMER_LEFT_4=86,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=59.639
PRIMER_RIGHT_4_TM=60.061
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_03228 Ps_contig_mira-and-tgicl-ass_franssen_29230
SEQUENCE=ACGTCCACTTCACTTTGTNAATGATTTTACATTTACATAATGTAAATTATATGTTTAAATTTGACTGACTGTAATCTCTTGTTTATTCTTATTTTTCTATCACCGCTTCAAAAATCAGTAGCATTGCAATCATCATCATCATCCATGGCTCCCAAAAGGGTTCTTCTCCTTTGCGGTGACTTCATGGAAGACTATGAAGCTATGGTTCCTTTTCAGGCATTGCAAGCATTCGGTATCACCGTCGATGCTGTTTGTCCCGGTAAAAAATCCGGTGAAGTCTGCCGCACCGCCGTCCATATGCTTTCCGGTGGACAGACATATACTGAGACAGTTGGTCACAATTTTACGCTGAATGCAACTTTTGATGAAATTGATCACACAAACTATGATGGGTTGTGGTTGCCGGGAGGAAGAGCGCCGGAGTATCTTGCTCACATTCCTAGTGTTGTGGAGCTGGTTGCCAAGTTTGTGAATTCTGGAAAACAGATTGCTTGCATTTGTCATGGCCTTTTGATTCTGGCAGCTGCTGGTGTAGTTGAAGGTCGAAAGTGCACAGCTTTTCCTCCTGTCAAACCGGTATTGGTTGCGGCTGGTGCTCATTGGGTTGAACCTGAGACAATGTCAACAACTGTGGTGGATGGTAATCTCATTACTGCACCTACTTATGAAGGGCATCCGGAACTTCTTCGGCATTTTCTAAAGGCATTAGGAGGCAAAATAAGTGGCTATGATAAAAAGATTCTCTTTATTTGCGGGGATTACATGGAAGATTATGAGGTTAAGGTTCCTTTTCAGTCTCTTGAGGCTTTAGGATGCCATGTTGATGGAGTTTGCCCCTCAAAGAAGGCTGGGGACACTTGCCCAACTGCTGTTCATGATTTTGAAGGAGATCAGACTTACAGTGAGAAGCCAGGACACAATTTTGATTTAACTGCGACCTTTGATGACGTTGATCCTTCAGCCTACGATGCTCTTGTCATCCCTGGAGGTCGATCACCTGAGTACTTGTCATTGGATGAGTCCGTTATTTCCTTGGTGAAGCATTTCATGGAAGCCAATAAGCCAGTTGCATCAATCTGCCATGGCCAACAGATTTTAGCTGCTGGTGGTCTTCTCAAGGGAAGGAAATGTACTGCTTATCCAGCTGTGAAGCTTAATGTAGTTTTATCCGGAGCAACATGGCTGGAGCCTGACCCCATAAGCCGCTGCTTCACTGATGGGAATTTGGTTACTGGAGCTGCATGGCCAGGGCACCCTGAGTTCATTGCCCAGTTGATGGCACTACTCGGTATTCAAGTATCATTCTAGTAGCCTTTTGTAATGTAGTATATTACAGTATAATAATGAATAAAAATCACCACACTTGTCCATTATCAATGGGCTCAACATAAGTGGAGATGTGTACTGTCACCATGTTGAATTTGAAGAACATGTAATGTGCTGAGCATGCAAGCACTTTGATGAAAGTGTCTCTTTTGGGTATGAATTATTCTCTCTCCTAATGAAGAGATACATTGTATATTTATATTACAAGAAAAATAAATAAGGAAAACTCCTTTGTAACAAACTAACTAGCTCACAATAAGTTCATAACAAACATAGCTGCAACTTTTTATCTGTCTAATGTATAATACTATATCTTAATTCTCTTGTTGAAATCGAACACCACATTAGTCAT
TARGET=129,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6373
PRIMER_LEFT_PENALTY=0.598109
PRIMER_RIGHT_PENALTY=0.039178
PRIMER_LEFT_SEQUENCE=TCACCGCTTCAAAAATCAGTAG
PRIMER_RIGHT_SEQUENCE=TCAGCGTAAAATTGTGACCAAC
PRIMER_LEFT=99,22
PRIMER_RIGHT=351,22
PRIMER_LEFT_TM=59.402
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.6535
PRIMER_LEFT_1_PENALTY=0.614307
PRIMER_RIGHT_1_PENALTY=0.039178
PRIMER_LEFT_1_SEQUENCE=CCGCTTCAAAAATCAGTAGCAT
PRIMER_RIGHT_1_SEQUENCE=TCAGCGTAAAATTGTGACCAAC
PRIMER_LEFT_1=102,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=60.614
PRIMER_RIGHT_1_TM=60.039
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.7228
PRIMER_LEFT_2_PENALTY=0.598109
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=TCACCGCTTCAAAAATCAGTAG
PRIMER_RIGHT_2_SEQUENCE=CAGTATATGTCTGTCCACCGGA
PRIMER_LEFT_2=99,22
PRIMER_RIGHT_2=324,22
PRIMER_LEFT_2_TM=59.402
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.7390
PRIMER_LEFT_3_PENALTY=0.614307
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=CCGCTTCAAAAATCAGTAGCAT
PRIMER_RIGHT_3_SEQUENCE=CAGTATATGTCTGTCCACCGGA
PRIMER_LEFT_3=102,22
PRIMER_RIGHT_3=324,22
PRIMER_LEFT_3_TM=60.614
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=1.0795
PRIMER_LEFT_4_PENALTY=0.598109
PRIMER_RIGHT_4_PENALTY=0.481423
PRIMER_LEFT_4_SEQUENCE=TCACCGCTTCAAAAATCAGTAG
PRIMER_RIGHT_4_SEQUENCE=CATTCAGCGTAAAATTGTGACC
PRIMER_LEFT_4=99,22
PRIMER_RIGHT_4=354,22
PRIMER_LEFT_4_TM=59.402
PRIMER_RIGHT_4_TM=59.519
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_03229 Ps_contig_mira-and-tgicl-ass_franssen_29233
SEQUENCE=TTAAACGTTTTTTTACCTTTTACGTCTTTCCTAAAAGGACAACCCAAAACAACTTTTCTANTTCACACACACAACAAATNACTTCTCTTCTTTCTACACAAATACAGCAGCCACCTATCACAATCACTCTTGATCTCTCCYYCACGCNACCTCACATTTTCTCATCACCATAAACCCATTCCAACCTTCAATTTGCTTTGAACATCCCTTCTCTCTCCATTCACAGGGAAGAAAAAACGGTTCACTCTTGTTACACAACCGCCATCTTCCTCGACACCACACTAACACCTCATTTTCGCTTCCGCCGAAACAACCTAGAATAACCGCCGTGAATCGCCACCACTACCACTACGTCGCCGCATCATCCCTCAACCACCGTCAATTTTGAACCATCCGCCATCTTCCTCATATATTCCGGCGATAAATTCCAGACCTCATCGCCGTGTCAATAACCGAACTCCTTCACACTCAGGCGAATCCACCTCTGTTCACAGACATCGCCGCCGCCACGGTTTGCTCTTCTAAATTCACCACAACCAACCGTGACGCAGATTCTCTCTTCCCCAACAGGCAAAACCAAGCAACAAAACTTCCAACTCCGCAGCAACAACAAACGACAGCAGTGACACCGCTTCGGTAAAAATATCAATTTCTTATGCTTGTGGTGTTGTGATTTTGAGATTAACTCTGTTATTACATTAAATTTTTATTGATTTCTGTTGTGTTACAAACTGTTGCAAGTATTATAGAGTGCAGATGTTCTGAGATTGATTCCATTAGGTAGTGTTTTTTGAATATGACTATGGCCTCATTGTTTTCTACTGGTTTT
TARGET=64,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03230 Ps_contig_mira-and-tgicl-ass_franssen_29235
SEQUENCE=TTAGATATTTTTACCGTAAGCGGTGTCACYTGCTGTCGTTTGTTGTCGCTGCGGAGTTGGAAGGTTTGTTGCTTGGTTTCTCCTGTTGGGGAAGAGAGAATCTGCGTCGCGGTTGGTTGTGGTGAATTTAGAAGAGCAAACCGTGGCGGCGGCGATGTCGGTGAACAAAGGTGGATTCGCCTGAGTGTGAAGGAGTTCGGTTATTGACACGGCGGTGAGGTCTGGAATTTATCGCCGGAATATATGAGGAAGACGGAGGATGGTTCAAAATTGACGGTGGTTGAGCGATGATGCGGCGACGTAGTGGTAGTGGTGGCGATTCACGGCGGTTATTCTAGGTTGTTTCGGCGGAAGCGAAAATGAGGTGTTAGTGTGGTGAAAGGGTGAAAGACGATGGAGTATTTGAAGAAGAGGAATGGTGATGGTTAAGGGTGAGGCGCTCAGGGAAATACCCAGAGGCTTCTTCTCTTTTCCATTTTTAATCCTTTTCCTTATTTCTTTTGAGAGAGAGAGTAAGTTAGTGTCTCTTGGTCCCTTTGTGAAGTCGGTAGAG
TARGET=503,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03231 Ps_contig_mira-and-tgicl-ass_franssen_29236
SEQUENCE=AGAATCTGCGTCGCGGGTTGGTTGTGGTGAATTTAGAAGAGCAAATCGTGGCGGCGGCGATGTCGGTGAACAGAGGTGGATTCCCCTAAGTGTGAAGGAGTTCGGTTATTGACACGACGGTGAGGTCTGGAATTTAGCGCCGGAATATATGAGGAAGACGGCGGATGGTTCAAAGTTGACGGTGGTTGAGCGATGATGCGGCGATGTAGTGGTGGCGATTCACGGCGGTTATTCTAGGTTGTTTCGGCGGAAGCGAAAATGAGGTGTTAGTGTGGTGAAAGGGTGGAAGACGATGGAGTATTTGAAGAAGAGGAATGGTGATGGTTAAGGGTGAGACGCTCAGGGAAAGACCCAAAGGCTTCTTCTCTTTTTCCATTTTTAATCCTTTTCTTTATTCTTTGAGAGAGAGAGTAAGTTAATGTATGTTGGTCCCCCG
TARGET=401,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1057
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=0.996198
PRIMER_LEFT_SEQUENCE=AATGGTGATGGTTAAGGGTGAG
PRIMER_RIGHT_SEQUENCE=GGGGGACCAACATACATTAACT
PRIMER_LEFT=313,22
PRIMER_RIGHT=434,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.004
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=122
PRIMER_PAIR_PENALTY_1=1.1253
PRIMER_LEFT_1_PENALTY=0.129103
PRIMER_RIGHT_1_PENALTY=0.996198
PRIMER_LEFT_1_SEQUENCE=GGAAGCGAAAATGAGGTGTTAG
PRIMER_RIGHT_1_SEQUENCE=GGGGGACCAACATACATTAACT
PRIMER_LEFT_1=248,22
PRIMER_RIGHT_1=434,22
PRIMER_LEFT_1_TM=60.129
PRIMER_RIGHT_1_TM=59.004
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=1.1845
PRIMER_LEFT_2_PENALTY=0.188254
PRIMER_RIGHT_2_PENALTY=0.996198
PRIMER_LEFT_2_SEQUENCE=GGTGTTAGTGTGGTGAAAGGGT
PRIMER_RIGHT_2_SEQUENCE=GGGGGACCAACATACATTAACT
PRIMER_LEFT_2=262,22
PRIMER_RIGHT_2=434,22
PRIMER_LEFT_2_TM=60.188
PRIMER_RIGHT_2_TM=59.004
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=1.3110
PRIMER_LEFT_3_PENALTY=0.314775
PRIMER_RIGHT_3_PENALTY=0.996198
PRIMER_LEFT_3_SEQUENCE=AGGTGTTAGTGTGGTGAAAGGG
PRIMER_RIGHT_3_SEQUENCE=GGGGGACCAACATACATTAACT
PRIMER_LEFT_3=261,22
PRIMER_RIGHT_3=434,22
PRIMER_LEFT_3_TM=60.315
PRIMER_RIGHT_3_TM=59.004
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=1.3339
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=1.224349
PRIMER_LEFT_4_SEQUENCE=AATGGTGATGGTTAAGGGTGAG
PRIMER_RIGHT_4_SEQUENCE=GGGGGACCAACATACATTAACTT
PRIMER_LEFT_4=313,22
PRIMER_RIGHT_4=434,23
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=60.224
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=122
=
PRIMER_SEQUENCE_ID=SSR_03232 Ps_contig_mira-and-tgicl-ass_franssen_29238
SEQUENCE=GTTCTGTCAAGCTAGATGTTATTATTTCTTTTGTAAAAGCAGAAAACTTATCATTTGTAAACAAGACTATTTCTTTTGTAAACAAGAATATCATTATTTCTTCGTGATCGAATGTCTAGCCCTTTCTTATTTTTGAAAAAGCAGACAACTTTATTCTCCAGATTAATATTTGCCTAAACAGGCATCAAGTATTCTTTAACAATGGGAGGCCTATTTGTACAGAAAGTATTGGCATATGTGTAGTATATTTATTGTCAAGCTGATTCAAGGCTTTCCTGAGTAACCCCACGGGTTGTTCTTTGCCCAATTCCACTGGTCCCTGCACATTTCCTCTACACCATATTTGGCCTTCCAACCAAGTTCTTTCTCAGCTTTCGCTGTGGATGCATAAACCTCTGTAGCATCTCCTGGCCTTCTTGGACACAATTTCAATGCAATTTTCTTGCCAGAAGCTTTCTCAAATGCAGCAACCATTTCAAGCACAGATGAACCACGACCAGTTCCCAAATTATAAGCAGTACAACCAATGTTTTCTGATGTGAAAAGCTTTCTCAGGGCAGCAATGTGACCATCTGCTAAGTCCATCACATGGATATAGTCCCGTATCGCAGAGCCATCCCTTGTAGGATAATCATGACCATATACATTGAGCTCAGGTAATCTTCCAACGGCTACTTGCTGTATATAAGGCATGAGATTATTTGGGATGCCTCTGGGATCTTCACCAAGTTTACCACTTTCATGTGCCCCGACTGGATTGAAGTACCGCAGTAAAACGATTCTCCATTCTGGCTCAGCTTTCTGAATATCTCGTGCAATTTCTTCAAGGAAGAGCTTGGTCCGTCCATAAGGGTTCATGGCTTGTAACTTGAAATCCTCCACGCAGGGTATCTTTTCAGGTTGGCCATAAACAGTTGCAGATGACGAGAAAACCATCTTTTTACAATTATGCTTAGCCATAACTTCATAGAGATTGATAGTTCCAACAAGATTATTATCGAAATAACGACGAGGATTTTCAACACTCTCACCAACTGCTTTTAGTCCAGCAAAGTGAATCACAGCATCAAATTTAGATTTTGAGAAGAGTTTCTCCAAATCATCTTTATTCCTGAGATCTCCCAGAGTGAATTCGAGATTCTGAGAGAGGTTAGAACCAACAACTTCACGGACGCGCTCCACTGCTTCCATAACAGAATTATCGAAATTATCAATGATGGAAACGTTGAAGCCGTTATTTAGAAGCTGAACCACCGTGTGAGTGCCGATGAAACCGGCACCGCCGGTTACGAGTATCTTTTGCGACGAAGCCACCATGTTTTGGAGAGAGAGAGAGAAGTTGTGAAATGTGAAAAAGTCCCCGA
TARGET=1324,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3184
PRIMER_LEFT_PENALTY=0.211806
PRIMER_RIGHT_PENALTY=1.106581
PRIMER_LEFT_SEQUENCE=GATCTCCCAGAGTGAATTCGAG
PRIMER_RIGHT_SEQUENCE=GGGGACTTTTTCACATTTCACA
PRIMER_LEFT=1115,22
PRIMER_RIGHT=1361,22
PRIMER_LEFT_TM=60.212
PRIMER_RIGHT_TM=61.107
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=247
PRIMER_PAIR_PENALTY_1=1.4071
PRIMER_LEFT_1_PENALTY=0.300539
PRIMER_RIGHT_1_PENALTY=1.106581
PRIMER_LEFT_1_SEQUENCE=ATTCCTGAGATCTCCCAGAGTG
PRIMER_RIGHT_1_SEQUENCE=GGGGACTTTTTCACATTTCACA
PRIMER_LEFT_1=1107,22
PRIMER_RIGHT_1=1361,22
PRIMER_LEFT_1_TM=59.699
PRIMER_RIGHT_1_TM=61.107
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=1.4071
PRIMER_LEFT_2_PENALTY=0.300539
PRIMER_RIGHT_2_PENALTY=1.106581
PRIMER_LEFT_2_SEQUENCE=CCTGAGATCTCCCAGAGTGAAT
PRIMER_RIGHT_2_SEQUENCE=GGGGACTTTTTCACATTTCACA
PRIMER_LEFT_2=1110,22
PRIMER_RIGHT_2=1361,22
PRIMER_LEFT_2_TM=59.699
PRIMER_RIGHT_2_TM=61.107
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=1.4578
PRIMER_LEFT_3_PENALTY=0.351175
PRIMER_RIGHT_3_PENALTY=1.106581
PRIMER_LEFT_3_SEQUENCE=TTAGAACCAACAACTTCACGGA
PRIMER_RIGHT_3_SEQUENCE=GGGGACTTTTTCACATTTCACA
PRIMER_LEFT_3=1150,22
PRIMER_RIGHT_3=1361,22
PRIMER_LEFT_3_TM=59.649
PRIMER_RIGHT_3_TM=61.107
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=1.7285
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=1.106581
PRIMER_LEFT_4_SEQUENCE=CTCCACTGCTTCCATAACAGAA
PRIMER_RIGHT_4_SEQUENCE=GGGGACTTTTTCACATTTCACA
PRIMER_LEFT_4=1176,22
PRIMER_RIGHT_4=1361,22
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=61.107
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_03233 Ps_contig_mira-and-tgicl-ass_franssen_29239
SEQUENCE=TGATATATGTTGAGAGCCATTTAAGTCTAACTTATCATCCAAATTCTATGGAGAAGTTACTCAACAGATGTTGAAGCATGCTGATTTTCCTGGTAGCAACAATGGAACAGGTCTGTTTCAAACCATTGTTGGTTTGAAGATAAGAGATTTGTTTGAGCAGATAGTAGCAAGCAGGGCTGAGACTCCAGTAGGAGCTGCTAAAGCTTAGGAAGAACTTGCCTTATATATACATAATATTATTGAAAAGGAGTAATTGAATTTTATTCTGAAAAAATAATTTCTTTACTTTTTGTGTATTCCACAATTCCTGTATTGTATTGTTTGTTTTGTGTATAGAAAAGTACTTTGTCTTGTTATTTATTTATGTAATGTATATGTTATTACTTATAATATAG
TARGET=354,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03234 Ps_contig_mira-and-tgicl-ass_franssen_29242
SEQUENCE=CTCATATATTTTATGTGTTCCAAACTTTTTCTGAGAATAAATGTCCCTTATCATTATTAATCCTNGATCCAACATAAACTCATTTCTTCTTCAAAATTTTCTTTAACTTAGAAAAAAGAGAGAGAGAAAAAGAAATCTCTCAGAAACAATGGCAGCTTGCACTGTCTACTCAACACAAACTCTAAGAACAAACATCTCAATTCCAACATCATCAAAAACAAACGTTGGACTCCATCAAAAACAAGTACTTTTCTTCACAACAAACCAGAAAAACAACAGCAGAAGAACAAACAGCAACAAAAGATACTTGATAACATGTGGAGCAGGTGACTCACAGACAATTGTGATTGGTCTAGCAGCCGATTCCGGTTGTGGTAAAAGTACTTTCATGAGAAGACTCACAAGTGTTTTTGGAGGAGCAGCAGAGCCACCAAAAGGTGGTAATCCTGATTCTAACACACTTATCAGTGACACAACAACTGTCATATGTTTGGATGATTATCATTCTTTGGATAGAACCGGTAGAAAAGAGAAAGGTGTTACTGCACTTGATCCAAAAGCTAATGATTTTGATCTCATGTATGAACAAGTTAAAGCTATTAAAGATGGAAAATCTGTTCAAAAACCTATTTATAATCATGTTACTGGTCTTTTGGATGCTCCTGAGCTTATTAAACCTCCCAAAATCTTAGTCATTGAAGGTCTTCATCCAATGTATGATTCTAGAGTTAGAGAACTCTTGGACTTCAGTATTTACTTAGACATTAGTAATGAGGTGAAATTTGCTTGGAAAATTCAGAGAGACATGGCAGAACGTGGACACAGTCTTGAAAGTATCAAGGCTAGCATTGAAGCAAGGAAGCCTGATTTTGAAGCTTATATTGATCCACAAAAGCAATATGCAGATGCAGTGATAGAAGTATTACCAACTCAACTCATCCCCGATGATAACGAGGGAAAGATTTTACGAGTGAGGTTGATACAGAAAGAGGGTGTTAAATACTTTAGCCCAGTTTACTTGTTCGACGAAGGTTCAACAATTTCATGGATACCATGTGGAAGAAAACTCACATGCTCTTATCCCGGAATCAAGTTCTTCTACGGACCAGAAACATACAAAGGAAACGAGGTGTCAGTTGTGGAAATGGACGGACAATTTGACAGACTAGATGAACTGATATATGTTGAGAGCCATTTAAGCAACTTATCATCCAAATTCTATGGAGAAGTTACTCAACAGATGTTGAAGCATGCTGATTTTCCTGGTAGCAACAATGGAACAGGTCTGTTTCAAACCATTGTTGGTTTGAAGATAAGAGATTTGTTTGAGCAGATAGTAGCAAGCAGGGCTGAGACTCCAGTAGGAGCTGCTAAAGCTTAGGAAGAACTTGCCTTATATATACATAATATTATTGAAAAGGGAGTAATTGAATTTTATTCTGAAAAAATAATTTCTTACTTTTTGTGTATTCCACAATTCCTGTATTGTATGTTGTTTTGTGTATGAAAAGACTTTGCTTGTATTTATTTATGTAATGTAATATGTTGTTACTTATAATATAGAGGAGGAGAAACAAGACTGAGATTTCAGTTATGTTCATGTACCAATTGCACTAAAACT
TARGET=117,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03235 Ps_contig_mira-and-tgicl-ass_franssen_29248
SEQUENCE=GCTGGTTTGAGTGTTATAAGTCGGTTGGCTGAAGAATTTGAGCAAAGGAGTCAAGTATTTGGTGATGATGCCAAGTTCTTGGTTGAGGTAAAATCTGGTCAAATTGAAGCAAGTCTGAACCCAGACAGAGAGCTTAGAAGGGTAAACAATGTTGAGGTAGGAAAAGGAACTATGCAACCAGATTGTCGTACGAAAACGTAAAAGGTTTAAAGTTTTTAAAATTTAAAACGTTAACGGTAAAGTAAGTAAGTAAGTGGTTACGT
TARGET=240,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03236 Ps_contig_mira-and-tgicl-ass_franssen_29254
SEQUENCE=ACGGNCAANGCARNGTCGACTCACTCACTCACATTTTTCTCTCAAAAACAAAATCTGTTCTTTCTCTCCGCTTCATTCTTCTCTCTTCGATTTCAATTCCTTTCATTTCTTCAAAAATGGCCAAAGATCCAGTTCGTGTTCTCGTCACTGGTGCCGCAGGACAAATTGGTTATGCTCTTGTCCCTATGATTGCTAGGGGAGTGATGTTGGGTCCAGATCAACCTGTGATCCTTCACATGCTTGATATTCCTCCTGCAGCTGAGTCATTGAATGGAGTTAAGATGGAGTTGGTGGATGCCGCATTTCCTCTTCTCAAAGGTGTTGTTGCTACAACTGATGTTGTCGAGGCATGCACTGGAGTCAATATTGCAGTCATGGTTGGTGGATTCCCAAGAAAAGAAGGCATGGAAAGGAAGGATGTGATGTCTAAGAATGTCTCTATCTACAAGTCCCAGGCTTCTGCCCTTGAAAAGCATGCTGCTGCCAACTGCAAGGTTCTGGTTGTTGCTAACCCAGCAAACACCAATGCATTGATCTTGAAGGAATTTGCTCCATCTATTCCCGAGAAAAACATTACTGCTTTGACTAGACTTGATCACAATAGGGCATTGGGCCAAATTTCGGAAAGATTGAACATTCAAGTTTCTGATGTTAAGAATGTCATTATCTGGGGTAATCATTCATCAACGCAGTATCCTGATGTCAACCATGCAACTGTTAACACCTCCGCCGGGGAGAAGCCTGTCCGTGAACTTGTTTCCGACGATTCCTGGTTGAATGGAGAATTCATATCTACGGTTCAACAACGTGGTGCTGCAATTATAAAGGCTAGAAAGCTTTCAAGCGCACTATCTGCTGCTAGTTCTGCTTGTGACCACATTCGCAATTGGGTTCTTGGAACTCCCGAGGGCACCTATGTTTCAATGGGAGTGTATTCTGATGGTTCTTACAATGTACCCGCCGGACTTATCTATTCATTCCCTGTCACTTGTGCCAATGGAGAATGGAAAATTGTTCAAGGACTTTCAATTGACGAGTTCTCAAGGAAGAAGCTGGACTTGACAGCCGAAGAGCTTTCTGAGGAAAAGAATTTGGCTTACTCTTGCCTCTCTTAGGCTGCTTTCTTCTTGTCTACATATTCCCATGTTTTTGTCTAGAGAAGTAGCTTCAGTTTTGAATAATGCTTCTAGATACAGTGTCTCTTTGGAATTACTTGTACTGCAAGTTTCACGGGTTTTAGCCATGATGGCGATACAAGAATTTTGATGTTTGTTGGCACTGCACAAGCGTGCAGGCACATACATCTATTCCTTGTGATTGAGTATTATGCAAGTGTATTGCAAAATAAAGCCATCATAAAATGTTTCATTATGAAGTTCGAGAACTTATTATAATGAAAAGAGTTCNAATTTTTAAACCTTT
TARGET=19,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03237 Ps_contig_mira-and-tgicl-ass_franssen_29256
SEQUENCE=CTCCCTANAATCAATTTCCGTGTTACCTCACCTGACCTATCTTTCTCAACAATCAAAACAACCATTTCAATTTTTCCATTACTATAAAACAACATTTTCTCTTTCACAAAAAAAAACCCTAAAACCCATTTCATCTTCATCTTCATCTATCAATTCAATTCAATTCAACCATGTCTCGATTCAGTAACAACCTAATCGGAATCCTCAATTTCCTAACCCTACTCCTCTCAATCCCAATTCTAGTCATGGGAGTATGGCTCCACAAACAGTCCACCACGGAATGCGAACGCTTCTTAGAGAAACCCATTATTGCCCTTGGCGTTTTCCTCTTGGTTGTTTCTCTCATGGGTTTAATCGGTGCTTGTTGCAGAGTCTCATGGCTTCTGTGGTTCTATCTTCTCGTCATGTTCTTGCTTATTGTTCTTCTTTTCGCTTTCACGATCTTTGCGTTTGTTGTTACTAACAAAGGTGCGGGTGAAGCTTTGTCGAATCGGGGGTATAAGGAGTATAGGCTTGGGGATTATTCGAATTGGTTGCAGAATAAGGTTACCAATGGGAATACTTGGGAGAGGATTAGGGGTTGTTTGGAATCGGGGAAGCTTTGCTCTGAATATCATTTCAAGTTTTTGAATGATAATATCGAGAAATTTCATACTGAACAATTGTCTGCTCTTCAGTCTGGATGTTGTAAGCCTTCAGAGGACTGTGGTTTTACTTATGTGAGCCCTACAAACTGGACGCCCGGAAATGTTAATTCTACAAACCTTGACTGTAAAGCTTGGAACAACGATCCGAAGATTCTGTGTTTTGATTGCCAATCTTGCAAGGCTGGGTTACTGCAAAACCTCAAGACTGATTGGAAGAAGGTGGCCGTAGTCAACATCATATTCCTGATTTTCCTGGTCATTGTATACTCCATCGGCTGCTGCGCATTCAGGAATAACAGGCAGGACAATTGGAAGAGATATTAGGCTCTTGAGCATTATTAGCATGTAGATGATTTAGGCTATATGGTTTATATGCTGATTTTGTGTGTGTGGATTTCATACTGTTTTTCAGTACAGTTTGACTGTTTGTGTAAATTGCTAAACTTTATAGTATTACAGTATGTGGATTGAAATTGACTTCAAATTAACCTTTGGTGCAATCCTATAGTTATAC
TARGET=150,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4394
PRIMER_LEFT_PENALTY=0.274768
PRIMER_RIGHT_PENALTY=0.164639
PRIMER_LEFT_SEQUENCE=CAATTTCCGTGTTACCTCACCT
PRIMER_RIGHT_SEQUENCE=TGAGGATTCCGATTAGGTTGTT
PRIMER_LEFT=11,22
PRIMER_RIGHT=206,22
PRIMER_LEFT_TM=60.275
PRIMER_RIGHT_TM=59.835
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.4394
PRIMER_LEFT_1_PENALTY=0.274768
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=CAATTTCCGTGTTACCTCACCT
PRIMER_RIGHT_1_SEQUENCE=TTGAGGATTCCGATTAGGTTGT
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=207,22
PRIMER_LEFT_1_TM=60.275
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=0.4394
PRIMER_LEFT_2_PENALTY=0.274768
PRIMER_RIGHT_2_PENALTY=0.164639
PRIMER_LEFT_2_SEQUENCE=AATTTCCGTGTTACCTCACCTG
PRIMER_RIGHT_2_SEQUENCE=TGAGGATTCCGATTAGGTTGTT
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=206,22
PRIMER_LEFT_2_TM=60.275
PRIMER_RIGHT_2_TM=59.835
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.4394
PRIMER_LEFT_3_PENALTY=0.274768
PRIMER_RIGHT_3_PENALTY=0.164639
PRIMER_LEFT_3_SEQUENCE=AATTTCCGTGTTACCTCACCTG
PRIMER_RIGHT_3_SEQUENCE=TTGAGGATTCCGATTAGGTTGT
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=207,22
PRIMER_LEFT_3_TM=60.275
PRIMER_RIGHT_3_TM=59.835
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=0.6088
PRIMER_LEFT_4_PENALTY=0.274768
PRIMER_RIGHT_4_PENALTY=0.334070
PRIMER_LEFT_4_SEQUENCE=CAATTTCCGTGTTACCTCACCT
PRIMER_RIGHT_4_SEQUENCE=TCCCATGACTAGAATTGGGATT
PRIMER_LEFT_4=11,22
PRIMER_RIGHT_4=250,22
PRIMER_LEFT_4_TM=60.275
PRIMER_RIGHT_4_TM=59.666
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_03238 Ps_contig_mira-and-tgicl-ass_franssen_29256
SEQUENCE=CTCCCTANAATCAATTTCCGTGTTACCTCACCTGACCTATCTTTCTCAACAATCAAAACAACCATTTCAATTTTTCCATTACTATAAAACAACATTTTCTCTTTCACAAAAAAAAACCCTAAAACCCATTTCATCTTCATCTTCATCTATCAATTCAATTCAATTCAACCATGTCTCGATTCAGTAACAACCTAATCGGAATCCTCAATTTCCTAACCCTACTCCTCTCAATCCCAATTCTAGTCATGGGAGTATGGCTCCACAAACAGTCCACCACGGAATGCGAACGCTTCTTAGAGAAACCCATTATTGCCCTTGGCGTTTTCCTCTTGGTTGTTTCTCTCATGGGTTTAATCGGTGCTTGTTGCAGAGTCTCATGGCTTCTGTGGTTCTATCTTCTCGTCATGTTCTTGCTTATTGTTCTTCTTTTCGCTTTCACGATCTTTGCGTTTGTTGTTACTAACAAAGGTGCGGGTGAAGCTTTGTCGAATCGGGGGTATAAGGAGTATAGGCTTGGGGATTATTCGAATTGGTTGCAGAATAAGGTTACCAATGGGAATACTTGGGAGAGGATTAGGGGTTGTTTGGAATCGGGGAAGCTTTGCTCTGAATATCATTTCAAGTTTTTGAATGATAATATCGAGAAATTTCATACTGAACAATTGTCTGCTCTTCAGTCTGGATGTTGTAAGCCTTCAGAGGACTGTGGTTTTACTTATGTGAGCCCTACAAACTGGACGCCCGGAAATGTTAATTCTACAAACCTTGACTGTAAAGCTTGGAACAACGATCCGAAGATTCTGTGTTTTGATTGCCAATCTTGCAAGGCTGGGTTACTGCAAAACCTCAAGACTGATTGGAAGAAGGTGGCCGTAGTCAACATCATATTCCTGATTTTCCTGGTCATTGTATACTCCATCGGCTGCTGCGCATTCAGGAATAACAGGCAGGACAATTGGAAGAGATATTAGGCTCTTGAGCATTATTAGCATGTAGATGATTTAGGCTATATGGTTTATATGCTGATTTTGTGTGTGTGGATTTCATACTGTTTTTCAGTACAGTTTGACTGTTTGTGTAAATTGCTAAACTTTATAGTATTACAGTATGTGGATTGAAATTGACTTCAAATTAACCTTTGGTGCAATCCTATAGTTATAC
TARGET=1030,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.6588
PRIMER_LEFT_PENALTY=0.013714
PRIMER_RIGHT_PENALTY=2.645058
PRIMER_LEFT_SEQUENCE=ATTGTATACTCCATCGGCTGCT
PRIMER_RIGHT_SEQUENCE=CTATAGGATTGCACCAAAGGTT
PRIMER_LEFT=905,22
PRIMER_RIGHT=1154,22
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=57.355
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=2.6588
PRIMER_LEFT_1_PENALTY=0.013714
PRIMER_RIGHT_1_PENALTY=2.645058
PRIMER_LEFT_1_SEQUENCE=ATTGTATACTCCATCGGCTGCT
PRIMER_RIGHT_1_SEQUENCE=AACTATAGGATTGCACCAAAGG
PRIMER_LEFT_1=905,22
PRIMER_RIGHT_1=1156,22
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=57.355
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=2.6635
PRIMER_LEFT_2_PENALTY=0.018437
PRIMER_RIGHT_2_PENALTY=2.645058
PRIMER_LEFT_2_SEQUENCE=TCATTGTATACTCCATCGGCTG
PRIMER_RIGHT_2_SEQUENCE=CTATAGGATTGCACCAAAGGTT
PRIMER_LEFT_2=903,22
PRIMER_RIGHT_2=1154,22
PRIMER_LEFT_2_TM=59.982
PRIMER_RIGHT_2_TM=57.355
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=2.6635
PRIMER_LEFT_3_PENALTY=0.018437
PRIMER_RIGHT_3_PENALTY=2.645058
PRIMER_LEFT_3_SEQUENCE=TCATTGTATACTCCATCGGCTG
PRIMER_RIGHT_3_SEQUENCE=AACTATAGGATTGCACCAAAGG
PRIMER_LEFT_3=903,22
PRIMER_RIGHT_3=1156,22
PRIMER_LEFT_3_TM=59.982
PRIMER_RIGHT_3_TM=57.355
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=2.9375
PRIMER_LEFT_4_PENALTY=0.292414
PRIMER_RIGHT_4_PENALTY=2.645058
PRIMER_LEFT_4_SEQUENCE=TGGTCATTGTATACTCCATCGG
PRIMER_RIGHT_4_SEQUENCE=CTATAGGATTGCACCAAAGGTT
PRIMER_LEFT_4=900,22
PRIMER_RIGHT_4=1154,22
PRIMER_LEFT_4_TM=59.708
PRIMER_RIGHT_4_TM=57.355
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.8000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_03239 Ps_contig_mira-and-tgicl-ass_franssen_29258
SEQUENCE=GTCGGGGACCAATATCTTTGTGGCTACAAAAAACAAACTCAACTTCATGATTCTCAGTTACCATTCCTGCTCCTTCTCTTTAACATCTCCCCTTAATAAATCATACCCCGTTCTTCCTTCTCTCAAACTAACAAAACCTTTACGCATACACTGCACACCACCACAACAACAACACCAACAACCCTTATCCACCATGGATGATGAAATAGCCATTGATCTAACCCCAATCGTAAAAGTATACAAAAACGGTCGTATTGAGAGGCTTATAGGTGAAGAGGTTGTTCCTGCATCCCTTGATCCAACCACCAACGTTGAATCCAAAGATGTTATAATCTCCAAAGAAGATAACATATCAGCGAGGCTTTACATTCCCAAAACCAACTACCCACCAACCCAAAAACTCCCTGTTTTTGTTTACATCCATGGTGGTGCTTTCTGCGTCGAAACCCCTTTCTCACCCAATTACCATAACTACCTTAACTCCGTTGCTTCACTGGCTAACGTAATTGGTGTCTCTGTTCACTATAGAAGAGCACCGGAACACCCTGTTCCTATCGCTCATGAAGATTCATGGCTTGCACTCAAATGGGTGGCTTCACATGTTGGTGGAAAGGGTTCTGATGAATGGTTGAATCAGCATGCAGATTTTGAGAAAGTGTTCTTAGGTGGTGACAGTGCTGGTGCTAATATTGCACACTATTTGGGTATTCGGGTCGGAAAGGAAAATTTGGATGATTTGAAACTTGAAGGGGGTGTTTTTGTTCATCCTTATTTTTGGGGTGTGGATCGAATTGGGTCTGAATCGGCTCGAGGTGAATTTGTTGAAAAAATTCATACTTTATGGCGATTTGCATGTCCAACCACGACTGGATCCGATGACCCGTTAATTAACCCGGAAAAGGATCCGAATTTGGGGAGTTTGGGTTTTAAGAGAGTGCTTGTTTGTGTAGCTGGGAAAGATTTGTTGAAGGATAGAGGTTGGTATTATAAAGAGTTACTTGAGAAAATTGGTTGGGGTGGTGTTGTTGATGTTGTTGAGACAAAGGATGAGCATCATGTTTTTCATATGTTTGACCCAACATGTGAGAATGCTAAAAATTTGCTTAATCAAGTTGTTTCCTTCATCAAAAGAACTTGAAAATATGAGGGTGGGAGTGATGCTTTCCTTTTATTTTTGTTTTCTATGTTTCTATTATCTATTTTTAAATTATTTTTTATGTACTCTTTCTTGTGTAGTTAAAAGAAAAAATACCTAAA
TARGET=163,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1817
PRIMER_LEFT_PENALTY=0.032476
PRIMER_RIGHT_PENALTY=0.149184
PRIMER_LEFT_SEQUENCE=ATACCCCGTTCTTCCTTCTCTC
PRIMER_RIGHT_SEQUENCE=TAAGCCTCTCAATACGACCGTT
PRIMER_LEFT=102,22
PRIMER_RIGHT=265,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.149
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.2249
PRIMER_LEFT_1_PENALTY=0.075753
PRIMER_RIGHT_1_PENALTY=0.149184
PRIMER_LEFT_1_SEQUENCE=AAATCATACCCCGTTCTTCCTT
PRIMER_RIGHT_1_SEQUENCE=TAAGCCTCTCAATACGACCGTT
PRIMER_LEFT_1=97,22
PRIMER_RIGHT_1=265,22
PRIMER_LEFT_1_TM=60.076
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.2748
PRIMER_LEFT_2_PENALTY=0.032476
PRIMER_RIGHT_2_PENALTY=0.242357
PRIMER_LEFT_2_SEQUENCE=ATACCCCGTTCTTCCTTCTCTC
PRIMER_RIGHT_2_SEQUENCE=GTTGGTTTTGGGAATGTAAAGC
PRIMER_LEFT_2=102,22
PRIMER_RIGHT_2=381,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=59.758
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.2795
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.149184
PRIMER_LEFT_3_SEQUENCE=CAGTTACCATTCCTGCTCCTTC
PRIMER_RIGHT_3_SEQUENCE=TAAGCCTCTCAATACGACCGTT
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=265,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.2795
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.149184
PRIMER_LEFT_4_SEQUENCE=TCAGTTACCATTCCTGCTCCTT
PRIMER_RIGHT_4_SEQUENCE=TAAGCCTCTCAATACGACCGTT
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=265,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=60.149
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_03240 Ps_contig_mira-and-tgicl-ass_franssen_29260
SEQUENCE=TCAACGCAGAGTACTTTTTCCTTTCTCTTTGTCTCTTTCTTTTTCTCTTTCTTTTCCTCGTGCTTTATGCTAGTACAAGTAATTACCCAATCTCATTCATAGCCACATTTTCTTCACCTCTTCTTATCTATATAACCATTTACAATCTCCACTTCCTTTTTCTTCATCATCCATAAAATCTTCACTATCTTTCTTCCATTCCAGAACTTGTATCATCATCATCAAAATCTTTGAATGTTTGGGTATTCACCCCGTGTTGACGCGTTGTAGACATCACGGCTTTTCTTAGAGTTTATCGCTCTCTTTCTCATGCCCGCACTTCACGACGTAAGGGTTCTTCTTTAACTCCTACTAACCCTTCGCCGCACGGTGGTCGTGGTGCTCTGCCTTCTGAAGGTGGTAGCCCCTCCGATCTCCTCTTCCTTGCTGGTGGTGGTTTCTTTGCTTCTTCCTCTTCTCTCTCTTTCTTCTAGTTTCTCTCTCTCTCTCTAGTTTAACTTTCTCTTTGTAAGTTAGATTTCCTTGAAGGTGCTTCTTTTTCTTCTTGTTTCTGTTGTTACTGTTGTTGCTGTTGGTGTTGTTGAATCTTAAAAGACAAAAAAAGTTTTTTTTTGTGTTTGTTTGTGAAAATGATGCTGAAAATCAAAAGGGTTCCTACGGTTGTTTCGAATTATCAAAAAGAGGAAGGTTCTGAGGCTCCTCGTACTGTCGGAGGTTGTGGTCGGAATTGCCTCAAAGCTTGTTGTATTCAAGATGCGAAGCTTCCTCTGTATGCTTTTAAGAAGATTAACAAAGTTGGTGGAAAGGATTTGGTGGCTGAGAAGTGCCAAGAGGAGCTTCCTTTGGCCTTTTTGGACTCACTTGTTCTTGGAGAGTGGGAAGATCGCGTGCAAAGAGGACTTTTTCGTTATGATGTTACCGCCTGTGAAACCAAGGTGATTCCGGGTGAGTGTGGTTTCATTGCTCAGCTCAACGAGGGTCGTCACCTCAAGAAGAGACCAACCGAGTTCAGAGTCGATAAGGTTCTTCAGCCTTTTGACGGAAACAAATTCAACTTCACTAAAGTTGGACAAGAGGAGGTTCTGTTTCAATTTGAGGCTAGTGAAGACGGTGAAGTCCAGTTCCATCCAAATGCTCCGATTGATGTTGACAATTCTCCAAGTTTCGTTGCCATCAACGTTAGTCCTATTGAGTATGGGCATGTTTTGCTGATTCCTCGCATTTTCGAGTGTTTGCCTCAAAGGATTGATAACGAGAGCTTATTGCTTGCACTTCACATGGCAGCTGAAGCCGCTAATCCATATTTCCGATTGGGTTACAACAGCCTCGGGGCTTTTGCAACTATTAACCATCTTCACTTCCAGGCTTATTATTTGGCTATGCCCTTTCCTATCGAGAAAGCTCCCACTAAGAAAATTGCAACTTTGAAAGGCGGAGTGAAGATGTCTGTATTGTTGAAATATCCGGTTCGAGGTCTTGTCTTCGAGGGCGGCGACACACTTGAAGATTTATCAGAAGTTGTTTCAGATGCTTGTATCTCCCTACAAAACAACAACATACCTTACAATGTTCTTATTTCTGACTGCGGAACGCAAGTGTTTCTGTTACCACAGTGTTATGCAGAGAAACAAGCTCTCGGAGAAGTGAATGCTGAGCTTCTCGACACACAAGTGAATCCCGCGGTGTGGGAGATTAGTGGACACATGGTTTTGAAGAGGAAAAAGGACTTCGACGAGGCGTCTGAAGCCAATGCTTGGAGGCTTCTTGCTGAAGTTTCACTCTCCGAAGAGAGGTTTCAAGAAGTCAACGCTCTTATCTTTGAAGCCATTGCCTTAACTGAAGAATCCGATGAAAACAATCTTCAGTGTCTTCCTAAGGACGACGCAGTCGACGACACAAGCACATACCCTACCGTGGTGGCTGGTTCGCAAGAATGTGTTGTTTTACAGTAACAACAGCAATGTTCTAAATGAATAAATTCAGTGATTGTGTGGTGAAAATTTGCTGAATTATGTCTAGTTGAAATAAGTACCTTCAATGGTAACACTTGTTAAGTTCTAGAATTATGTTATGCCTAATGTTTGTGTTTTGTATAACAACTATTGTGCTTTCTGTTATGGCTTTCATCTTTCAACTTGCTTGAAATAACAACTTCATGTAACTGCACTGCATTGAATTTCTAGATGCACATTTGTAATAAATGATTATTGTAATTTTTAATTANTTGTCAACTAGTCAATAAAAGT
TARGET=212,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4264
PRIMER_LEFT_PENALTY=0.116582
PRIMER_RIGHT_PENALTY=0.309780
PRIMER_LEFT_SEQUENCE=GCCACATTTTCTTCACCTCTTC
PRIMER_RIGHT_SEQUENCE=AAAGAAGAACCCTTACGTCGTG
PRIMER_LEFT=101,22
PRIMER_RIGHT=342,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=59.690
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.5250
PRIMER_LEFT_1_PENALTY=0.215225
PRIMER_RIGHT_1_PENALTY=0.309780
PRIMER_LEFT_1_SEQUENCE=ACCCAATCTCATTCATAGCCAC
PRIMER_RIGHT_1_SEQUENCE=AAAGAAGAACCCTTACGTCGTG
PRIMER_LEFT_1=84,22
PRIMER_RIGHT_1=342,22
PRIMER_LEFT_1_TM=60.215
PRIMER_RIGHT_1_TM=59.690
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.6233
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.622602
PRIMER_LEFT_2_SEQUENCE=TTTCTCTTTCTTTTCCTCGTGC
PRIMER_RIGHT_2_SEQUENCE=GTGATGTCTACAACGCGTCAAC
PRIMER_LEFT_2=41,22
PRIMER_RIGHT_2=276,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.623
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.6233
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.622602
PRIMER_LEFT_3_SEQUENCE=TTTCTCTTTCTTTTCCTCGTGC
PRIMER_RIGHT_3_SEQUENCE=GATGTCTACAACGCGTCAACAC
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=274,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.623
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.6569
PRIMER_LEFT_4_PENALTY=0.347129
PRIMER_RIGHT_4_PENALTY=0.309780
PRIMER_LEFT_4_SEQUENCE=TCTCTTTCTTTTCCTCGTGCTT
PRIMER_RIGHT_4_SEQUENCE=AAAGAAGAACCCTTACGTCGTG
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=342,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=59.690
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=300
=
PRIMER_SEQUENCE_ID=SSR_03241 Ps_contig_mira-and-tgicl-ass_franssen_29260
SEQUENCE=TCAACGCAGAGTACTTTTTCCTTTCTCTTTGTCTCTTTCTTTTTCTCTTTCTTTTCCTCGTGCTTTATGCTAGTACAAGTAATTACCCAATCTCATTCATAGCCACATTTTCTTCACCTCTTCTTATCTATATAACCATTTACAATCTCCACTTCCTTTTTCTTCATCATCCATAAAATCTTCACTATCTTTCTTCCATTCCAGAACTTGTATCATCATCATCAAAATCTTTGAATGTTTGGGTATTCACCCCGTGTTGACGCGTTGTAGACATCACGGCTTTTCTTAGAGTTTATCGCTCTCTTTCTCATGCCCGCACTTCACGACGTAAGGGTTCTTCTTTAACTCCTACTAACCCTTCGCCGCACGGTGGTCGTGGTGCTCTGCCTTCTGAAGGTGGTAGCCCCTCCGATCTCCTCTTCCTTGCTGGTGGTGGTTTCTTTGCTTCTTCCTCTTCTCTCTCTTTCTTCTAGTTTCTCTCTCTCTCTCTAGTTTAACTTTCTCTTTGTAAGTTAGATTTCCTTGAAGGTGCTTCTTTTTCTTCTTGTTTCTGTTGTTACTGTTGTTGCTGTTGGTGTTGTTGAATCTTAAAAGACAAAAAAAGTTTTTTTTTGTGTTTGTTTGTGAAAATGATGCTGAAAATCAAAAGGGTTCCTACGGTTGTTTCGAATTATCAAAAAGAGGAAGGTTCTGAGGCTCCTCGTACTGTCGGAGGTTGTGGTCGGAATTGCCTCAAAGCTTGTTGTATTCAAGATGCGAAGCTTCCTCTGTATGCTTTTAAGAAGATTAACAAAGTTGGTGGAAAGGATTTGGTGGCTGAGAAGTGCCAAGAGGAGCTTCCTTTGGCCTTTTTGGACTCACTTGTTCTTGGAGAGTGGGAAGATCGCGTGCAAAGAGGACTTTTTCGTTATGATGTTACCGCCTGTGAAACCAAGGTGATTCCGGGTGAGTGTGGTTTCATTGCTCAGCTCAACGAGGGTCGTCACCTCAAGAAGAGACCAACCGAGTTCAGAGTCGATAAGGTTCTTCAGCCTTTTGACGGAAACAAATTCAACTTCACTAAAGTTGGACAAGAGGAGGTTCTGTTTCAATTTGAGGCTAGTGAAGACGGTGAAGTCCAGTTCCATCCAAATGCTCCGATTGATGTTGACAATTCTCCAAGTTTCGTTGCCATCAACGTTAGTCCTATTGAGTATGGGCATGTTTTGCTGATTCCTCGCATTTTCGAGTGTTTGCCTCAAAGGATTGATAACGAGAGCTTATTGCTTGCACTTCACATGGCAGCTGAAGCCGCTAATCCATATTTCCGATTGGGTTACAACAGCCTCGGGGCTTTTGCAACTATTAACCATCTTCACTTCCAGGCTTATTATTTGGCTATGCCCTTTCCTATCGAGAAAGCTCCCACTAAGAAAATTGCAACTTTGAAAGGCGGAGTGAAGATGTCTGTATTGTTGAAATATCCGGTTCGAGGTCTTGTCTTCGAGGGCGGCGACACACTTGAAGATTTATCAGAAGTTGTTTCAGATGCTTGTATCTCCCTACAAAACAACAACATACCTTACAATGTTCTTATTTCTGACTGCGGAACGCAAGTGTTTCTGTTACCACAGTGTTATGCAGAGAAACAAGCTCTCGGAGAAGTGAATGCTGAGCTTCTCGACACACAAGTGAATCCCGCGGTGTGGGAGATTAGTGGACACATGGTTTTGAAGAGGAAAAAGGACTTCGACGAGGCGTCTGAAGCCAATGCTTGGAGGCTTCTTGCTGAAGTTTCACTCTCCGAAGAGAGGTTTCAAGAAGTCAACGCTCTTATCTTTGAAGCCATTGCCTTAACTGAAGAATCCGATGAAAACAATCTTCAGTGTCTTCCTAAGGACGACGCAGTCGACGACACAAGCACATACCCTACCGTGGTGGCTGGTTCGCAAGAATGTGTTGTTTTACAGTAACAACAGCAATGTTCTAAATGAATAAATTCAGTGATTGTGTGGTGAAAATTTGCTGAATTATGTCTAGTTGAAATAAGTACCTTCAATGGTAACACTTGTTAAGTTCTAGAATTATGTTATGCCTAATGTTTGTGTTTTGTATAACAACTATTGTGCTTTCTGTTATGGCTTTCATCTTTCAACTTGCTTGAAATAACAACTTCATGTAACTGCACTGCATTGAATTTCTAGATGCACATTTGTAATAAATGATTATTGTAATTTTTAATTANTTGTCAACTAGTCAATAAAAGT
TARGET=476,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2085
PRIMER_LEFT_PENALTY=0.151106
PRIMER_RIGHT_PENALTY=0.057411
PRIMER_LEFT_SEQUENCE=GTGGTGGTTTCTTTGCTTCTTC
PRIMER_RIGHT_SEQUENCE=ATTCAACAACACCAACAGCAAC
PRIMER_LEFT=429,22
PRIMER_RIGHT=585,22
PRIMER_LEFT_TM=60.151
PRIMER_RIGHT_TM=59.943
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.2220
PRIMER_LEFT_1_PENALTY=0.164560
PRIMER_RIGHT_1_PENALTY=0.057411
PRIMER_LEFT_1_SEQUENCE=TTCACGACGTAAGGGTTCTTCT
PRIMER_RIGHT_1_SEQUENCE=ATTCAACAACACCAACAGCAAC
PRIMER_LEFT_1=319,22
PRIMER_RIGHT_1=585,22
PRIMER_LEFT_1_TM=60.165
PRIMER_RIGHT_1_TM=59.943
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.2220
PRIMER_LEFT_2_PENALTY=0.164560
PRIMER_RIGHT_2_PENALTY=0.057411
PRIMER_LEFT_2_SEQUENCE=CTTCACGACGTAAGGGTTCTTC
PRIMER_RIGHT_2_SEQUENCE=ATTCAACAACACCAACAGCAAC
PRIMER_LEFT_2=318,22
PRIMER_RIGHT_2=585,22
PRIMER_LEFT_2_TM=60.165
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.2220
PRIMER_LEFT_3_PENALTY=0.164560
PRIMER_RIGHT_3_PENALTY=0.057411
PRIMER_LEFT_3_SEQUENCE=TCACGACGTAAGGGTTCTTCTT
PRIMER_RIGHT_3_SEQUENCE=ATTCAACAACACCAACAGCAAC
PRIMER_LEFT_3=320,22
PRIMER_RIGHT_3=585,22
PRIMER_LEFT_3_TM=60.165
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=0.2563
PRIMER_LEFT_4_PENALTY=0.151106
PRIMER_RIGHT_4_PENALTY=0.105201
PRIMER_LEFT_4_SEQUENCE=GTGGTGGTTTCTTTGCTTCTTC
PRIMER_RIGHT_4_SEQUENCE=AGCCTCAGAACCTTCCTCTTTT
PRIMER_LEFT_4=429,22
PRIMER_RIGHT_4=697,22
PRIMER_LEFT_4_TM=60.151
PRIMER_RIGHT_4_TM=59.895
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_03242 Ps_contig_mira-and-tgicl-ass_franssen_29262
SEQUENCE=GTTCGGGGAGAAATTTTGGATTAGATAGATAGATAGGGGTGTCAAGTGGCAAATTCTTCAAAAGCTATACCAACACAAACACAAACCAATTCCTACTGTATCTCAATCAAAAAATGGCCACACTATTTTCATCTTCATCTCTTCATTCAATCCGTCTTTCTTCTTCTACATTCCCCGTTTCTTCCTCTTCTTCTCAATCCCTTCAATTTTGCACTTTTCCATGCCACCCTCGTTTGAAGGCACTGTCAATAAAAAAGAACCGTTGTTTACTTGTGGTTAAGGCAGTGGAAGATGAAACCCAACAACAACAAGAACAAGATGTTACTGAGATTGAACAACAGAAACAACCATCATCAACCTCGGAGCAACAACCATCATCAACCTCGGAGCAACAACCAGTCGTTGTTCCCATTTCACCATCAGATACCCTCACAATGCTCTTTCAGGCAGAGGGAACATTGAAGGAAACAGCCGTTCCACCATTGACCAAAGCTTTAGAGGAGATCGAGGGTGTTAAAAATTTGAAGATTAATGTATTCGAGGGACTCGCAACTTTAGAGTTGGAAAAGCAGACAACAATCCAAGCAACAGGAGTGGCTTCTGGTATTGTAGAAACCATACAAGGTTTAGGCTTCAAGTTACAGACTTTAAATCTGAGTTTTGATGATGAAGATGTTGTTGCAGTTGCCTAGACAGACAAAATCTACTTGTGTAACAAACTGAACAAAATTTCTTGATTAAAGCCTACTGCACAAATTCATACAGTCCAGGCTTCGATTATCATCTGTAATTTTATAAATTATTTGATTTTGGTCGATCGACTATGGATATTTTGAATGCGTACGTGAATGTGGAATCTCTTGATTGCAGAGAATATGATTTTGNAAAGAATGTTGCATGC
TARGET=22,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3597
PRIMER_LEFT_PENALTY=2.350759
PRIMER_RIGHT_PENALTY=0.008930
PRIMER_LEFT_SEQUENCE=GTTCGGGGAGAAATTTTGGAT
PRIMER_RIGHT_SEQUENCE=TGAGAAGAAGAGGAAGAAACGG
PRIMER_LEFT=0,21
PRIMER_RIGHT=195,22
PRIMER_LEFT_TM=61.351
PRIMER_RIGHT_TM=59.991
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=2.4627
PRIMER_LEFT_1_PENALTY=2.453742
PRIMER_RIGHT_1_PENALTY=0.008930
PRIMER_LEFT_1_SEQUENCE=GTTCGGGGAGAAATTTTGGATT
PRIMER_RIGHT_1_SEQUENCE=TGAGAAGAAGAGGAAGAAACGG
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=195,22
PRIMER_LEFT_1_TM=62.454
PRIMER_RIGHT_1_TM=59.991
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=2.5163
PRIMER_LEFT_2_PENALTY=2.350759
PRIMER_RIGHT_2_PENALTY=0.165541
PRIMER_LEFT_2_SEQUENCE=GTTCGGGGAGAAATTTTGGAT
PRIMER_RIGHT_2_SEQUENCE=TTATTGACAGTGCCTTCAAACG
PRIMER_LEFT_2=0,21
PRIMER_RIGHT_2=251,22
PRIMER_LEFT_2_TM=61.351
PRIMER_RIGHT_2_TM=60.166
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=2.5302
PRIMER_LEFT_3_PENALTY=2.350759
PRIMER_RIGHT_3_PENALTY=0.179421
PRIMER_LEFT_3_SEQUENCE=GTTCGGGGAGAAATTTTGGAT
PRIMER_RIGHT_3_SEQUENCE=TGAAGGGATTGAGAAGAAGAGG
PRIMER_LEFT_3=0,21
PRIMER_RIGHT_3=204,22
PRIMER_LEFT_3_TM=61.351
PRIMER_RIGHT_3_TM=59.821
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=2.6193
PRIMER_LEFT_4_PENALTY=2.453742
PRIMER_RIGHT_4_PENALTY=0.165541
PRIMER_LEFT_4_SEQUENCE=GTTCGGGGAGAAATTTTGGATT
PRIMER_RIGHT_4_SEQUENCE=TTATTGACAGTGCCTTCAAACG
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=251,22
PRIMER_LEFT_4_TM=62.454
PRIMER_RIGHT_4_TM=60.166
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_03243 Ps_contig_mira-and-tgicl-ass_franssen_29262
SEQUENCE=GTTCGGGGAGAAATTTTGGATTAGATAGATAGATAGGGGTGTCAAGTGGCAAATTCTTCAAAAGCTATACCAACACAAACACAAACCAATTCCTACTGTATCTCAATCAAAAAATGGCCACACTATTTTCATCTTCATCTCTTCATTCAATCCGTCTTTCTTCTTCTACATTCCCCGTTTCTTCCTCTTCTTCTCAATCCCTTCAATTTTGCACTTTTCCATGCCACCCTCGTTTGAAGGCACTGTCAATAAAAAAGAACCGTTGTTTACTTGTGGTTAAGGCAGTGGAAGATGAAACCCAACAACAACAAGAACAAGATGTTACTGAGATTGAACAACAGAAACAACCATCATCAACCTCGGAGCAACAACCATCATCAACCTCGGAGCAACAACCAGTCGTTGTTCCCATTTCACCATCAGATACCCTCACAATGCTCTTTCAGGCAGAGGGAACATTGAAGGAAACAGCCGTTCCACCATTGACCAAAGCTTTAGAGGAGATCGAGGGTGTTAAAAATTTGAAGATTAATGTATTCGAGGGACTCGCAACTTTAGAGTTGGAAAAGCAGACAACAATCCAAGCAACAGGAGTGGCTTCTGGTATTGTAGAAACCATACAAGGTTTAGGCTTCAAGTTACAGACTTTAAATCTGAGTTTTGATGATGAAGATGTTGTTGCAGTTGCCTAGACAGACAAAATCTACTTGTGTAACAAACTGAACAAAATTTCTTGATTAAAGCCTACTGCACAAATTCATACAGTCCAGGCTTCGATTATCATCTGTAATTTTATAAATTATTTGATTTTGGTCGATCGACTATGGATATTTTGAATGCGTACGTGAATGTGGAATCTCTTGATTGCAGAGAATATGATTTTGNAAAGAATGTTGCATGC
TARGET=300,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0325
PRIMER_LEFT_PENALTY=0.008930
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=CCGTTTCTTCCTCTTCTTCTCA
PRIMER_RIGHT_SEQUENCE=CTGTTTCCTTCAATGTTCCCTC
PRIMER_LEFT=174,22
PRIMER_RIGHT=470,22
PRIMER_LEFT_TM=59.991
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.0325
PRIMER_LEFT_1_PENALTY=0.008930
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=CCGTTTCTTCCTCTTCTTCTCA
PRIMER_RIGHT_1_SEQUENCE=TGTTTCCTTCAATGTTCCCTCT
PRIMER_LEFT_1=174,22
PRIMER_RIGHT_1=469,22
PRIMER_LEFT_1_TM=59.991
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=0.0325
PRIMER_LEFT_2_PENALTY=0.008930
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=CCGTTTCTTCCTCTTCTTCTCA
PRIMER_RIGHT_2_SEQUENCE=GTTTCCTTCAATGTTCCCTCTG
PRIMER_LEFT_2=174,22
PRIMER_RIGHT_2=468,22
PRIMER_LEFT_2_TM=59.991
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.0834
PRIMER_LEFT_3_PENALTY=0.008930
PRIMER_RIGHT_3_PENALTY=0.074466
PRIMER_LEFT_3_SEQUENCE=CCGTTTCTTCCTCTTCTTCTCA
PRIMER_RIGHT_3_SEQUENCE=AGGGTATCTGATGGTGAAATGG
PRIMER_LEFT_3=174,22
PRIMER_RIGHT_3=429,22
PRIMER_LEFT_3_TM=59.991
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.1891
PRIMER_LEFT_4_PENALTY=0.165541
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=CGTTTGAAGGCACTGTCAATAA
PRIMER_RIGHT_4_SEQUENCE=GTTTCCTTCAATGTTCCCTCTG
PRIMER_LEFT_4=230,22
PRIMER_RIGHT_4=468,22
PRIMER_LEFT_4_TM=60.166
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_03244 Ps_contig_mira-and-tgicl-ass_franssen_29273
SEQUENCE=CAATTTATGAGGAATCGGAAACTTTGCACAAGGATTTTGGAAAGACGTTTGCGTTTTCTTCATCAGCATTGGAAGCTCCAGAGAAAGATTTTTTGCTTGGAGGAGATGTGACAACAAAGGTAAATAAAGGAAAGAGAGCGCTTAGTAGTGTGCGTGAAATGATCGATAACACGAAACTGCCTCATGGGGAATCGCATTTCGGTATGTTAATGGAGAATCTTGATGTTTTAGAAGAAACTTTTGCTGATTCTGAAGCATTGAGGTTGAAGAAGAATATTATATCGCAAATAGAAAAGCTTGGAGCTCTTGAGTTGTTCAATGATTGTCTAACAACATCTTTCGGAACCTCGCGCGTATCGAATTGCACTAACGAAGTTCTTGAACAAGTTGAAGAGAATAAGAGAAACTGCAAATTTGATGACTATACAGGTAAAGTTATTGTCCATTCTAGCAAGAGGAAGGAAAGTAGAACCAGAAGAAAGCGAATATCTGTTTCCATTGCACCTTCGTCTAAGTCGTTGCCGTTAGAAGATAATAATCAAGAAAATACATTACGTTCTTCGCCGGCTTCTTTTGTAAAAAAAGTATCATATACTAAAAATAGAAGAATGGCGATTGCTCAAAGGGAAGTTGAAATGTCAAAAGGAGTAAAGGTTTTAGCAGAGTTAGAGAAAATGAAAACAGCGTTAGAAGAGGATACAAAGCAAGTGGTAAGCTTGAGTAGCTGGGCGGAAGCATCCGGAGTCGAGGAGAAGATGCTACAACAGCAACTGTATCATGGCTATTATTGTAGAGACGAGTTGATACGGAGTACTCGTTCGTTAGTTGTATACTTTGCCAAAAAATACCGGGGTATGGGAATAGCTTTGGAAGATTTACTTCAGGTAGGGTATATAGGTGTTCTCCAAGGAGCCGAGCGATTCGACACTACAAGAGGATACCGATTCTCAACCTATGTGCAATACTGGATAAGGAAATCAATGTCAAGAGTGGTGGCAAAATATGCTCGAGGAATCACCGTTCCTTGGTCAATGAACAAGGCGATAAGTCAGATCCAGAAAGCACGAAAAGTCTTGAAAAACACGACCATGAAATACGCAGACGATCACGAGATTGCAAATATGACAGGTCTTTCTCTGGATAAAATCAGATCAGCTAGCCATTGTCTGAGAATAGTTTCCTCAATGAATCGGAATCAAGATATAGATTATTTGGGTCAAATGGCCGATAAGTCGATAGAGTGTCCTGAAGAGACCGTCATGAAACAACACTTTAGAAAAGACATATTTGATATCCTTCAAAGCTTGGACTCGAGGGAAAGACAAATACTAATCCTACGATTCGGCCTTAACGATCACCAACCCAGGTCCCTCGAGTATATAGGGAGGATTTTCAAAGTTAGCAAAGAGTGGATAAGGAAAATAGAGCAAAAAGCTCTCACAAAACTAAGGAATGAAACAAACATTTCAATGTTGAATTACTATTTGGATCTGCAGTAGTTTATCATTCAGTAGCATATATTTTCATCTCTATATGAGGGATGTGTACAAACTCTTAGTCCAAAGTTTATTGAATGCATTGTGTATTAAAGAAAGTTGTAGCTGCAGCATTTCACCACAAATTAATTTGGCTACATGGAAGAGAACTTGAATGAGTTTTATGTGGTCATTGTGAAACTCTACTGATAATATATATATTTATTTTGTTTGTGGCCTTGAGAGC
TARGET=1688,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1998
PRIMER_LEFT_PENALTY=1.685968
PRIMER_RIGHT_PENALTY=0.513831
PRIMER_LEFT_SEQUENCE=GAGCAAAAAGCTCTCACAAAAC
PRIMER_RIGHT_SEQUENCE=CTCTCAAGGCCACAAACAAAAT
PRIMER_LEFT=1424,22
PRIMER_RIGHT=1720,22
PRIMER_LEFT_TM=58.314
PRIMER_RIGHT_TM=60.514
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=2.3664
PRIMER_LEFT_1_PENALTY=1.852615
PRIMER_RIGHT_1_PENALTY=0.513831
PRIMER_LEFT_1_SEQUENCE=GTAGCTGCAGCATTTCACCAC
PRIMER_RIGHT_1_SEQUENCE=CTCTCAAGGCCACAAACAAAAT
PRIMER_LEFT_1=1597,21
PRIMER_RIGHT_1=1720,22
PRIMER_LEFT_1_TM=60.853
PRIMER_RIGHT_1_TM=60.514
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=2.5325
PRIMER_LEFT_2_PENALTY=2.018700
PRIMER_RIGHT_2_PENALTY=0.513831
PRIMER_LEFT_2_SEQUENCE=TAGCTGCAGCATTTCACCAC
PRIMER_RIGHT_2_SEQUENCE=CTCTCAAGGCCACAAACAAAAT
PRIMER_LEFT_2=1598,20
PRIMER_RIGHT_2=1720,22
PRIMER_LEFT_2_TM=60.019
PRIMER_RIGHT_2_TM=60.514
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=2.5325
PRIMER_LEFT_3_PENALTY=2.018700
PRIMER_RIGHT_3_PENALTY=0.513831
PRIMER_LEFT_3_SEQUENCE=GTAGCTGCAGCATTTCACCA
PRIMER_RIGHT_3_SEQUENCE=CTCTCAAGGCCACAAACAAAAT
PRIMER_LEFT_3=1597,20
PRIMER_RIGHT_3=1720,22
PRIMER_LEFT_3_TM=60.019
PRIMER_RIGHT_3_TM=60.514
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=2.5325
PRIMER_LEFT_4_PENALTY=2.018700
PRIMER_RIGHT_4_PENALTY=0.513831
PRIMER_LEFT_4_SEQUENCE=TGTAGCTGCAGCATTTCACC
PRIMER_RIGHT_4_SEQUENCE=CTCTCAAGGCCACAAACAAAAT
PRIMER_LEFT_4=1596,20
PRIMER_RIGHT_4=1720,22
PRIMER_LEFT_4_TM=60.019
PRIMER_RIGHT_4_TM=60.514
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_03245 Ps_contig_mira-and-tgicl-ass_franssen_29281
SEQUENCE=CTTCATCTTCTTCTTCTTCATCTCTCTGATCGGTTCAGCATGAACAGCTTTACAAACTCATCATTCCATTTCCTACTTTTCTTCAACCTTAATTCTGAACCAAAACAACTCCACCCTTAGAAATCTACAATTGTTCTTCAGAATAACATAACCAATTCTCTACAAGCAAAATCGGTTCTTCCCAGCTTCACTTGAGAGCCACCAATAACCGTAATTCCATGGCAAAGAACAAAAACTCTAATAATCACAGACAAAACTGGTTACCAAAAATATACAAAATTGGTAAACTTTTCAAGAACTACCCAGTTGTGAATTCGTAATAGAATTAGTTTTGG
TARGET=6,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03246 Ps_contig_mira-and-tgicl-ass_franssen_29301
SEQUENCE=AATTARCATGCAAACNNTTTTAACTCCAAACAAAATGTGAACTAGTTTCCAACTACAAAATAACCAGTCAAACGAAACAAGATAGTAGTAGTCGAGTTGACTTAATCAAACACTACAACATACATACTTGTCTTTTAAGTTTTGCATAAAACCCCATAAAGGATACAACAAGTCTACAAGGTGACCTAGATATATAAATAACCACCTAAATTAATAACGACTAACCAAGAAAAAGTCGTTGTCATCAAGAACCTGAACGTCTTGAGTTCCAACGAAATGCTGTCGACCAATTTCCTTCACCAAATTGGCAAATTCTATCCTTTTCTCCAAAGGGAGAGGTACAAATGGCAGCCTGAAAACTGGCCTGACAACTCCAAGTTGAGCAAGAGCTGTGTTCAAACCAATGGGATTTGGCATTTGGAAAAGCCAGGCAATCAAAGGCAAGAGCTTGGAATTCAATGCAGGGTTTTTCCCCCCAAACATGAGTTCATGCATTAAGCCAGGAATCAGGTTGCTTGCAACAGATACCACTCCAGTAGCTCCGTAACCCCATCTGGAATCATGGCATTCATCATCGTTCCCACTCCACACAACAATTTTATTATCAGTATACTCTTTGATCCGGTCATTTCCCATACATTCCTTGACACCAGCTAAGCAAGTACTTTGAGCTAAGTTTTGAATCACGTGTGGAGGAATGTCTTGACCAGTCCGTGAAGGAACATTGTATATGATTGTGGGTCCCATGGAAAGCACACTTTGAAAATGTGCAACCATACCATCCAAGGAGGTTTTTCCATAGTAAGGATTTATATGAAGGGCAGCATGCATTCCAACAGCAAAACCTTGTTCTGTGGCATGAATTGCTTCCCTGGTTGAGTTACTTCCAGTATTTCCAATAACCTTAATATTCCCACCAAAACAGTTGACTGTGTGTGCAATAAGCATTATGTGTTCTTCCCAGCTCATTAGTTGCCCTTCACCAGTTGTGCCACCAACAATAACACCTTCAACTCCATTCTCAATCTGCGTATTTACCAAAGCATCATATGCTTCAAGATCAAATCGACCATCAGGTAGGTATGGAGTTTTAATGGCAGTTATCAATCTCAGACACTTTATGTCTCTCAAAGTTGTATCCAACCCATAAGGTGTTTTGTCATATGTGGTTTCTGTTGAATTTAGAAACACTTTACATGATTCTCTTCTTTGAGCTATGGTTCCATGATAATGAAAATATTTTATTCTTCCTTCCGTGTTGTGAGTTGTTTTTCCATCTAAATTCTGTGACATGTGAACACCAGTTGCAAATAGCCTACGTGGTTGCACTGCATATTTTCTGTCCCTTCTAATCCCTTTCTTCACATCTTTGTACACCAGCTTTTCAATTCCCCATTTCCTATAGGTTTTTCCATAATCAGAAGAATGGCAAAGCTGGTTATTCATTGGCATCTGTTCAATTGTAAACTGAGAGTATTCCGATAGCGAATTGAGCACCGATTTAATCGTACTTTTAGGAGGAACATAGATATACTCATCAACATCAAAGAAGAACATCCATTTCCCCATAAATTTATACCTATGTAAACAATCATTCACAACCATAAACTGATTATGATAATACCCATCGAATCTCTCTTGATCCCTAATATCTTGTAGAGTCACATATCCAAGTTCAATCCATGGTTTCAAAACTTCATAAACCTCTGCATGAACACCACCAGCATCATGAATCACAAAATGTGAACTAGGTCCAAAGAATTTCACATGGTAAGCAATCCATTCACGAACTCTTTGTGGATTCAAGTTCCCGTAAAGCGATGAGCCACAATAGAAATAATCATACTTAGGCTTTGAAGTGAAAATCGAAAAATCTACCGTTCCGTTTGGTTCGTTAAGAACTTCGATTCTGTCGGTGATGTTTAAGCTTGTGTCACCGCCGCCGGAAGTTGAAGCGTAGAGAATCAACTTCCCGCCGGTGTTGTCGGTGTTTAGCGGCTCCGAGAAGGTACAGTTTATTATCACAACCGTGTAAACACGACCGTAGCCCCAGTCAGGAAGCATCTTGTAAGCAACGGTGGTGATTTTTCTCGAATCTCCGCCACCGGAGCCGGCGGAGATCCACTCACACTCGTAAGTCGGTTTACTATAAAGATGAAGAGGCTTTGAAGCTATACCGATAATGGCGAAAGTGTTTACGCCACCGCGATAAGCTCCCATGGTGACGAAGTTATAAGCAACAGAACCGTAAGGGTTAAAAGCGCGTTTGATAATCCCGTTTTCAAGCACACGTTCATTGTTAACTAACGGTGGAGGCGGTGGAGGTAGATTTGGAACAGAAGCTAACTGTTCAGTAACGGTAACAAACTTTTCCGTTACGGTTATGTTGTTTGCTGTGGCTGTGGTGTTAGGGAGTGTTTGGGTAACTCGTGAGATACAAAAGCGAATATCAGAAGCTGAAATAGTGAAACGAGAAGGGATGAATTGAAGGAAAGTAGCGACGGTGCAGAGAAGGAAAAGCGTTGTTATTAAGAGCTTAAGTTCCGCGGCGTAGTTGCCAAAAAGTGATATGAAGAGCTTTTTTTCTCTTTCTTTGGCCATTATGGTTTTTTCTTCTTCTTCTGCTTCTTTGGAGAAGAGAAGAGAAGAAGATAAGCACTTTTTTTGTTCTGCTTCTTTGGAGAAGAGAAGAAGAGAAGCGTCTTTTTTTTTGTCGGNCCNCGA
TARGET=2611,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03247 Ps_contig_mira-and-tgicl-ass_franssen_29301
SEQUENCE=AATTARCATGCAAACNNTTTTAACTCCAAACAAAATGTGAACTAGTTTCCAACTACAAAATAACCAGTCAAACGAAACAAGATAGTAGTAGTCGAGTTGACTTAATCAAACACTACAACATACATACTTGTCTTTTAAGTTTTGCATAAAACCCCATAAAGGATACAACAAGTCTACAAGGTGACCTAGATATATAAATAACCACCTAAATTAATAACGACTAACCAAGAAAAAGTCGTTGTCATCAAGAACCTGAACGTCTTGAGTTCCAACGAAATGCTGTCGACCAATTTCCTTCACCAAATTGGCAAATTCTATCCTTTTCTCCAAAGGGAGAGGTACAAATGGCAGCCTGAAAACTGGCCTGACAACTCCAAGTTGAGCAAGAGCTGTGTTCAAACCAATGGGATTTGGCATTTGGAAAAGCCAGGCAATCAAAGGCAAGAGCTTGGAATTCAATGCAGGGTTTTTCCCCCCAAACATGAGTTCATGCATTAAGCCAGGAATCAGGTTGCTTGCAACAGATACCACTCCAGTAGCTCCGTAACCCCATCTGGAATCATGGCATTCATCATCGTTCCCACTCCACACAACAATTTTATTATCAGTATACTCTTTGATCCGGTCATTTCCCATACATTCCTTGACACCAGCTAAGCAAGTACTTTGAGCTAAGTTTTGAATCACGTGTGGAGGAATGTCTTGACCAGTCCGTGAAGGAACATTGTATATGATTGTGGGTCCCATGGAAAGCACACTTTGAAAATGTGCAACCATACCATCCAAGGAGGTTTTTCCATAGTAAGGATTTATATGAAGGGCAGCATGCATTCCAACAGCAAAACCTTGTTCTGTGGCATGAATTGCTTCCCTGGTTGAGTTACTTCCAGTATTTCCAATAACCTTAATATTCCCACCAAAACAGTTGACTGTGTGTGCAATAAGCATTATGTGTTCTTCCCAGCTCATTAGTTGCCCTTCACCAGTTGTGCCACCAACAATAACACCTTCAACTCCATTCTCAATCTGCGTATTTACCAAAGCATCATATGCTTCAAGATCAAATCGACCATCAGGTAGGTATGGAGTTTTAATGGCAGTTATCAATCTCAGACACTTTATGTCTCTCAAAGTTGTATCCAACCCATAAGGTGTTTTGTCATATGTGGTTTCTGTTGAATTTAGAAACACTTTACATGATTCTCTTCTTTGAGCTATGGTTCCATGATAATGAAAATATTTTATTCTTCCTTCCGTGTTGTGAGTTGTTTTTCCATCTAAATTCTGTGACATGTGAACACCAGTTGCAAATAGCCTACGTGGTTGCACTGCATATTTTCTGTCCCTTCTAATCCCTTTCTTCACATCTTTGTACACCAGCTTTTCAATTCCCCATTTCCTATAGGTTTTTCCATAATCAGAAGAATGGCAAAGCTGGTTATTCATTGGCATCTGTTCAATTGTAAACTGAGAGTATTCCGATAGCGAATTGAGCACCGATTTAATCGTACTTTTAGGAGGAACATAGATATACTCATCAACATCAAAGAAGAACATCCATTTCCCCATAAATTTATACCTATGTAAACAATCATTCACAACCATAAACTGATTATGATAATACCCATCGAATCTCTCTTGATCCCTAATATCTTGTAGAGTCACATATCCAAGTTCAATCCATGGTTTCAAAACTTCATAAACCTCTGCATGAACACCACCAGCATCATGAATCACAAAATGTGAACTAGGTCCAAAGAATTTCACATGGTAAGCAATCCATTCACGAACTCTTTGTGGATTCAAGTTCCCGTAAAGCGATGAGCCACAATAGAAATAATCATACTTAGGCTTTGAAGTGAAAATCGAAAAATCTACCGTTCCGTTTGGTTCGTTAAGAACTTCGATTCTGTCGGTGATGTTTAAGCTTGTGTCACCGCCGCCGGAAGTTGAAGCGTAGAGAATCAACTTCCCGCCGGTGTTGTCGGTGTTTAGCGGCTCCGAGAAGGTACAGTTTATTATCACAACCGTGTAAACACGACCGTAGCCCCAGTCAGGAAGCATCTTGTAAGCAACGGTGGTGATTTTTCTCGAATCTCCGCCACCGGAGCCGGCGGAGATCCACTCACACTCGTAAGTCGGTTTACTATAAAGATGAAGAGGCTTTGAAGCTATACCGATAATGGCGAAAGTGTTTACGCCACCGCGATAAGCTCCCATGGTGACGAAGTTATAAGCAACAGAACCGTAAGGGTTAAAAGCGCGTTTGATAATCCCGTTTTCAAGCACACGTTCATTGTTAACTAACGGTGGAGGCGGTGGAGGTAGATTTGGAACAGAAGCTAACTGTTCAGTAACGGTAACAAACTTTTCCGTTACGGTTATGTTGTTTGCTGTGGCTGTGGTGTTAGGGAGTGTTTGGGTAACTCGTGAGATACAAAAGCGAATATCAGAAGCTGAAATAGTGAAACGAGAAGGGATGAATTGAAGGAAAGTAGCGACGGTGCAGAGAAGGAAAAGCGTTGTTATTAAGAGCTTAAGTTCCGCGGCGTAGTTGCCAAAAAGTGATATGAAGAGCTTTTTTTCTCTTTCTTTGGCCATTATGGTTTTTTCTTCTTCTTCTGCTTCTTTGGAGAAGAGAAGAGAAGAAGATAAGCACTTTTTTTGTTCTGCTTCTTTGGAGAAGAGAAGAAGAGAAGCGTCTTTTTTTTTGTCGGNCCNCGA
TARGET=2633,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03248 Ps_contig_mira-and-tgicl-ass_franssen_29302
SEQUENCE=AAAGACAAGTAGGTAATCAAACGCAGAGTACGTCGGGGAGTGTTTGTTTTTTTGGTCCTTAGAGAACTTGTTGAATTAATGGCTTCTACAACTGCTGCTTCTGCCTTACTTCAATTCCACTCTCTTTCTCCTAATCCTCAATTTTTTCTCACCAAACTCCCTAACTACCCAATCTCCCTTACTTCTTCTACCAAACGGTCTTCCAATTATAAGCGCGTTTACGCAAAAGCTGTTTCTACCGACTCACAAAAACCTCTCGCCCAAAATAATCAAAACACTTCCACATCCTCCTCCTCCAAACTGGTTCTTGTTCTTGGTGCCTCAGGAGGTGTAGGGCAGCTTGTTCTAGCGTCATTGCTTCAGCGGAATATTAAGTCGCGGGTGATACTTAGGGATCTTGAGAAAGCTAAGGCACTGTTTGGTGAACAAGATGAGGAGAGACTGCAGGTTTTCAAAGGAGACACAAGGAAGCATGAAGATTTGGAACCATCTATGTTTGAGGGAGTCACTCATGTGATTTGCTGCACAGGAACAACGGCTTTTCCTTCGAGGCGGTGGGATGATGAGAATACACCAGAAAGGGTTGATTGGGTGGGAGTGAAGAATCTAGTATCTGCATTGCCTTCCTCGGTGAAGAGAGTTGTTCTTGTGTCATCCATTGGTGTGACCAAGTGTAAAGAATTGCCATGGAGCATTATGAACCTATTTGGTGTCTTGAAGTATAAGAAGATGGGGGAGGATTTTCTTCAGAGCTCTGGCTTTCCATTTACCATAATTAGACCTGGAAGATTGACTGATGGACCATACACGTCTTATGATCTCAATACTTTACTCAAAGCAACAGTTGGTCAACGGCGAGCAGTCCTTATAGGTCAAGGAGATAAATTAGTGGGAGAGGCCAGCAGAATTGTGGTTGCTGAAGCTTGCATACAGGCACTAGATCTTGATGTCACTGAAAACCAAATATATGAAGTTAACTCTGTAGAGGGGGAAGGACCTGGAAATGATGCTCAGAAATGGCGAGAATTATTCGAGGCGGCACGATCTAGCTAACTAGACTTATTGTCAAAACTTGTATATCATTAAGAACCATGTATATGCATCATAGTTAGGGGCTTCAGCTTTGCAACTATTGTATAGATATATATATACACTTCTTTATTCATTATTGGATACACACAAAAGCACTTGCTTCATTAAAAANATTTCGCCTAAATTAATACACACAGAAGCTACTTTTCATAATGTAAAT
TARGET=286,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0580
PRIMER_LEFT_PENALTY=0.045851
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=GCAAAAGCTGTTTCTACCGACT
PRIMER_RIGHT_SEQUENCE=CCTTGTGTCTCCTTTGAAAACC
PRIMER_LEFT=222,22
PRIMER_RIGHT=467,22
PRIMER_LEFT_TM=59.954
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.0593
PRIMER_LEFT_1_PENALTY=0.045851
PRIMER_RIGHT_1_PENALTY=0.013401
PRIMER_LEFT_1_SEQUENCE=GCAAAAGCTGTTTCTACCGACT
PRIMER_RIGHT_1_SEQUENCE=TGCCTTAGCTTTCTCAAGATCC
PRIMER_LEFT_1=222,22
PRIMER_RIGHT_1=413,22
PRIMER_LEFT_1_TM=59.954
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.0979
PRIMER_LEFT_2_PENALTY=0.045851
PRIMER_RIGHT_2_PENALTY=0.052069
PRIMER_LEFT_2_SEQUENCE=GCAAAAGCTGTTTCTACCGACT
PRIMER_RIGHT_2_SEQUENCE=CCAAACAGTGCCTTAGCTTTCT
PRIMER_LEFT_2=222,22
PRIMER_RIGHT_2=421,22
PRIMER_LEFT_2_TM=59.954
PRIMER_RIGHT_2_TM=59.948
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=0.1420
PRIMER_LEFT_3_PENALTY=0.128591
PRIMER_RIGHT_3_PENALTY=0.013401
PRIMER_LEFT_3_SEQUENCE=TTCTACCAAACGGTCTTCCAAT
PRIMER_RIGHT_3_SEQUENCE=TGCCTTAGCTTTCTCAAGATCC
PRIMER_LEFT_3=185,22
PRIMER_RIGHT_3=413,22
PRIMER_LEFT_3_TM=59.871
PRIMER_RIGHT_3_TM=59.987
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=0.1610
PRIMER_LEFT_4_PENALTY=0.045851
PRIMER_RIGHT_4_PENALTY=0.115189
PRIMER_LEFT_4_SEQUENCE=GCAAAAGCTGTTTCTACCGACT
PRIMER_RIGHT_4_SEQUENCE=AAACCTGCAGTCTCTCCTCATC
PRIMER_LEFT_4=222,22
PRIMER_RIGHT_4=450,22
PRIMER_LEFT_4_TM=59.954
PRIMER_RIGHT_4_TM=59.885
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_03249 Ps_contig_mira-and-tgicl-ass_franssen_29302
SEQUENCE=AAAGACAAGTAGGTAATCAAACGCAGAGTACGTCGGGGAGTGTTTGTTTTTTTGGTCCTTAGAGAACTTGTTGAATTAATGGCTTCTACAACTGCTGCTTCTGCCTTACTTCAATTCCACTCTCTTTCTCCTAATCCTCAATTTTTTCTCACCAAACTCCCTAACTACCCAATCTCCCTTACTTCTTCTACCAAACGGTCTTCCAATTATAAGCGCGTTTACGCAAAAGCTGTTTCTACCGACTCACAAAAACCTCTCGCCCAAAATAATCAAAACACTTCCACATCCTCCTCCTCCAAACTGGTTCTTGTTCTTGGTGCCTCAGGAGGTGTAGGGCAGCTTGTTCTAGCGTCATTGCTTCAGCGGAATATTAAGTCGCGGGTGATACTTAGGGATCTTGAGAAAGCTAAGGCACTGTTTGGTGAACAAGATGAGGAGAGACTGCAGGTTTTCAAAGGAGACACAAGGAAGCATGAAGATTTGGAACCATCTATGTTTGAGGGAGTCACTCATGTGATTTGCTGCACAGGAACAACGGCTTTTCCTTCGAGGCGGTGGGATGATGAGAATACACCAGAAAGGGTTGATTGGGTGGGAGTGAAGAATCTAGTATCTGCATTGCCTTCCTCGGTGAAGAGAGTTGTTCTTGTGTCATCCATTGGTGTGACCAAGTGTAAAGAATTGCCATGGAGCATTATGAACCTATTTGGTGTCTTGAAGTATAAGAAGATGGGGGAGGATTTTCTTCAGAGCTCTGGCTTTCCATTTACCATAATTAGACCTGGAAGATTGACTGATGGACCATACACGTCTTATGATCTCAATACTTTACTCAAAGCAACAGTTGGTCAACGGCGAGCAGTCCTTATAGGTCAAGGAGATAAATTAGTGGGAGAGGCCAGCAGAATTGTGGTTGCTGAAGCTTGCATACAGGCACTAGATCTTGATGTCACTGAAAACCAAATATATGAAGTTAACTCTGTAGAGGGGGAAGGACCTGGAAATGATGCTCAGAAATGGCGAGAATTATTCGAGGCGGCACGATCTAGCTAACTAGACTTATTGTCAAAACTTGTATATCATTAAGAACCATGTATATGCATCATAGTTAGGGGCTTCAGCTTTGCAACTATTGTATAGATATATATATACACTTCTTTATTCATTATTGGATACACACAAAAGCACTTGCTTCATTAAAAANATTTCGCCTAAATTAATACACACAGAAGCTACTTTTCATAATGTAAAT
TARGET=1141,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3189
PRIMER_LEFT_PENALTY=0.001065
PRIMER_RIGHT_PENALTY=0.317786
PRIMER_LEFT_SEQUENCE=AACTCTGTAGAGGGGGAAGGAC
PRIMER_RIGHT_SEQUENCE=GCAAGTGCTTTTGTGTGTATCC
PRIMER_LEFT=975,22
PRIMER_RIGHT=1191,22
PRIMER_LEFT_TM=59.999
PRIMER_RIGHT_TM=59.682
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.3479
PRIMER_LEFT_1_PENALTY=0.001065
PRIMER_RIGHT_1_PENALTY=0.346860
PRIMER_LEFT_1_SEQUENCE=AACTCTGTAGAGGGGGAAGGAC
PRIMER_RIGHT_1_SEQUENCE=ATGAAGCAAGTGCTTTTGTGTG
PRIMER_LEFT_1=975,22
PRIMER_RIGHT_1=1196,22
PRIMER_LEFT_1_TM=59.999
PRIMER_RIGHT_1_TM=60.347
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.7981
PRIMER_LEFT_2_PENALTY=0.451213
PRIMER_RIGHT_2_PENALTY=0.346860
PRIMER_LEFT_2_SEQUENCE=CCTGGAAATGATGCTCAGAAAT
PRIMER_RIGHT_2_SEQUENCE=ATGAAGCAAGTGCTTTTGTGTG
PRIMER_LEFT_2=996,22
PRIMER_RIGHT_2=1196,22
PRIMER_LEFT_2_TM=60.451
PRIMER_RIGHT_2_TM=60.347
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.8575
PRIMER_LEFT_3_PENALTY=0.510616
PRIMER_RIGHT_3_PENALTY=0.346860
PRIMER_LEFT_3_SEQUENCE=ATGCATCATAGTTAGGGGCTTC
PRIMER_RIGHT_3_SEQUENCE=ATGAAGCAAGTGCTTTTGTGTG
PRIMER_LEFT_3=1097,22
PRIMER_RIGHT_3=1196,22
PRIMER_LEFT_3_TM=59.489
PRIMER_RIGHT_3_TM=60.347
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=0.8980
PRIMER_LEFT_4_PENALTY=0.001065
PRIMER_RIGHT_4_PENALTY=0.896968
PRIMER_LEFT_4_SEQUENCE=AACTCTGTAGAGGGGGAAGGAC
PRIMER_RIGHT_4_SEQUENCE=TGAAGCAAGTGCTTTTGTGTGT
PRIMER_LEFT_4=975,22
PRIMER_RIGHT_4=1195,22
PRIMER_LEFT_4_TM=59.999
PRIMER_RIGHT_4_TM=60.897
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_03250 Ps_contig_mira-and-tgicl-ass_franssen_29307
SEQUENCE=TTAAGAACAATAATCATGTTTGTTGATTGATTAAGACAGTGCTTGCTTTCATAATTGTTAGGGGGGTGTATAACAGAATCAACTCAATGGATTGAGAAAGCAAAAAGTAGTGTATAAATAAATTTAACAACAGTTACAGACAAGCTAACCAAAGATAACACAAAGTAAGAAGAAAAGAAAAACAAAAACCTTACAAGAGCAAGAGACTAATTCTTTGGAATGTTCTTGCCAACAATCTTGGCCGGAGCTATCCGCAAAAGATTCCCAGGCTTACCAGGAAGAGCACCTTTGATCATGATAACATTAAGCTCTTTATCAATTTTAACAATCTTAAGCTTTCTGATCTTTGTGTTGGATCCACCCATTCTCCCAGGCATCTTTTTTCCCTTGTATACCCGACCAGGTGTAGTTCCGGCACCAATTGAACCCAATTGTCTATGACTCTTGGAACCATGGCTCATCGGACCCCTCTTGAAGTTATATCGCTTGATTCCACCTTGGAAACCCTTGCCAATGGTGGTGCCGGAGACATCAACAAGATCACCCTCTTTGAAAAGTTCATCAAAGACAAGGAGCTGATTAGGTTGAAATCCGTCAACAGTTTGGAGACGAAACTCCTGAAGATGGCGCAAAGGAATGACTCCAGACTTTTTAAGATGACCCAATTCGGGTTGGGTCAATTTGGTGTCCCTAACCCTACGGTAACCGACTTGGACAGCGTTGTAACCGTCCGTGGCTTCGGTTTTGATCTGAGTGACAATGTTGCCTTCTTTGAAACCGACAATAGTAACGGGAACAACTTCTCCGTTAGGTTCAAAATAAGTCATCATTCCGAGTTTGGTAGCCATGACTCCAACGCCAGCCTCCATACTCATGACAACCACTCGTCTTTTTGGTCTATCATGTTGCTGCAATTTGAGGAATTGAGAAGAGGGATTCTTGGAAATTGAAGTTGAGAATGAAAGTGAAGTAGAACGGGAGGAGGAGGGTAATGAAATTGAGAATGAGGAGAATGCCGAAGAAATGGAAGCGAGCGACATGATGGATGGATGCTCTAAGTGTCAATGGTTAGTTAAGTTGTTATGTGAAAAGAGAGAGAGCCATCGACAACAATGCTTTAAAAACGAGTCTTATCTTCCYTACGNCCTACCCCGAC
TARGET=1041,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03251 Ps_contig_mira-and-tgicl-ass_franssen_29307
SEQUENCE=TTAAGAACAATAATCATGTTTGTTGATTGATTAAGACAGTGCTTGCTTTCATAATTGTTAGGGGGGTGTATAACAGAATCAACTCAATGGATTGAGAAAGCAAAAAGTAGTGTATAAATAAATTTAACAACAGTTACAGACAAGCTAACCAAAGATAACACAAAGTAAGAAGAAAAGAAAAACAAAAACCTTACAAGAGCAAGAGACTAATTCTTTGGAATGTTCTTGCCAACAATCTTGGCCGGAGCTATCCGCAAAAGATTCCCAGGCTTACCAGGAAGAGCACCTTTGATCATGATAACATTAAGCTCTTTATCAATTTTAACAATCTTAAGCTTTCTGATCTTTGTGTTGGATCCACCCATTCTCCCAGGCATCTTTTTTCCCTTGTATACCCGACCAGGTGTAGTTCCGGCACCAATTGAACCCAATTGTCTATGACTCTTGGAACCATGGCTCATCGGACCCCTCTTGAAGTTATATCGCTTGATTCCACCTTGGAAACCCTTGCCAATGGTGGTGCCGGAGACATCAACAAGATCACCCTCTTTGAAAAGTTCATCAAAGACAAGGAGCTGATTAGGTTGAAATCCGTCAACAGTTTGGAGACGAAACTCCTGAAGATGGCGCAAAGGAATGACTCCAGACTTTTTAAGATGACCCAATTCGGGTTGGGTCAATTTGGTGTCCCTAACCCTACGGTAACCGACTTGGACAGCGTTGTAACCGTCCGTGGCTTCGGTTTTGATCTGAGTGACAATGTTGCCTTCTTTGAAACCGACAATAGTAACGGGAACAACTTCTCCGTTAGGTTCAAAATAAGTCATCATTCCGAGTTTGGTAGCCATGACTCCAACGCCAGCCTCCATACTCATGACAACCACTCGTCTTTTTGGTCTATCATGTTGCTGCAATTTGAGGAATTGAGAAGAGGGATTCTTGGAAATTGAAGTTGAGAATGAAAGTGAAGTAGAACGGGAGGAGGAGGGTAATGAAATTGAGAATGAGGAGAATGCCGAAGAAATGGAAGCGAGCGACATGATGGATGGATGCTCTAAGTGTCAATGGTTAGTTAAGTTGTTATGTGAAAAGAGAGAGAGCCATCGACAACAATGCTTTAAAAACGAGTCTTATCTTCCYTACGNCCTACCCCGAC
TARGET=1091,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03252 Ps_contig_mira-and-tgicl-ass_franssen_29310
SEQUENCE=GGGGATTCAGTACTTTGTGTTGTTTGATTGTGTCTTAAATCAGCTTTTGTTGTTTGTTCTTGTGTTTGATCCATTTCATCCTCTGAAAATGTCTTGCTGTGGTGGAAACTGTGGTTGCGGTACTAGCTGCAAGTGCGGCTCTGGCTGCGGAGGGTGCAAGATGTATGCTGATTTGAGCTACACCGAGACAACAAGCTCTGAGACACTGATCATGGGAGTTGGATCTGAGAAGACTCAATTTGAGAGTGCTGAGATGGGAGCTGAGAATGATGGCTGCAAATGTGGAGCAAACTGCACTTGCAACCCTTGCACCTGCAAATGAAGTGGTTTTTACCCCGTTTAGTGGCAGGATAATAAGTTATGATTATGTACTAGTATGTTTTATGTGAACTCTGGTGTTTTGTTTAAATGTCAAACGCCCTTTTGGAGTTATAGAATAAAATAAAGTTGTTTGTTATGTTTAAAATCTTCAATCAAAATCCATTGTTTTGGATTTGTGATGGGTGTGTATTTTAAGTTCGGCTTAACTATGGTTTCTTTGTTTGATTATGCTTGTTTGTTTGTTTTTTGGATTAAAGTTCGGCTAATATGAACCATTAAAATTGTTAAAAAAGTTTCTGCCTTGTTTTCATAAAAACTGTATACTTGTTGTCAGAAACAGGGAAAATAAATGCTCATTGAAATGTGC
TARGET=553,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=6.0454
PRIMER_LEFT_PENALTY=0.221785
PRIMER_RIGHT_PENALTY=5.823654
PRIMER_LEFT_SEQUENCE=GGGTGTGTATTTTAAGTTCGGC
PRIMER_RIGHT_SEQUENCE=CCTGTTTCTGACAACAAGTATACAG
PRIMER_LEFT=501,22
PRIMER_RIGHT=661,25
PRIMER_LEFT_TM=59.778
PRIMER_RIGHT_TM=57.176
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=6.6391
PRIMER_LEFT_1_PENALTY=0.815487
PRIMER_RIGHT_1_PENALTY=5.823654
PRIMER_LEFT_1_SEQUENCE=AACGCCCTTTTGGAGTTATAGA
PRIMER_RIGHT_1_SEQUENCE=CCTGTTTCTGACAACAAGTATACAG
PRIMER_LEFT_1=414,22
PRIMER_RIGHT_1=661,25
PRIMER_LEFT_1_TM=59.185
PRIMER_RIGHT_1_TM=57.176
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=6.6987
PRIMER_LEFT_2_PENALTY=0.875033
PRIMER_RIGHT_2_PENALTY=5.823654
PRIMER_LEFT_2_SEQUENCE=GTTTTGGATTTGTGATGGGTGT
PRIMER_RIGHT_2_SEQUENCE=CCTGTTTCTGACAACAAGTATACAG
PRIMER_LEFT_2=485,22
PRIMER_RIGHT_2=661,25
PRIMER_LEFT_2_TM=60.875
PRIMER_RIGHT_2_TM=57.176
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=6.8642
PRIMER_LEFT_3_PENALTY=1.040509
PRIMER_RIGHT_3_PENALTY=5.823654
PRIMER_LEFT_3_SEQUENCE=TTGGATTTGTGATGGGTGTGTA
PRIMER_RIGHT_3_SEQUENCE=CCTGTTTCTGACAACAAGTATACAG
PRIMER_LEFT_3=488,22
PRIMER_RIGHT_3=661,25
PRIMER_LEFT_3_TM=61.041
PRIMER_RIGHT_3_TM=57.176
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=6.9044
PRIMER_LEFT_4_PENALTY=1.080765
PRIMER_RIGHT_4_PENALTY=5.823654
PRIMER_LEFT_4_SEQUENCE=TGGGTGTGTATTTTAAGTTCGG
PRIMER_RIGHT_4_SEQUENCE=CCTGTTTCTGACAACAAGTATACAG
PRIMER_LEFT_4=500,22
PRIMER_RIGHT_4=661,25
PRIMER_LEFT_4_TM=58.919
PRIMER_RIGHT_4_TM=57.176
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_03253 Ps_contig_mira-and-tgicl-ass_franssen_29313
SEQUENCE=GGGACGGCTCCCTCCTCTCTCTCTCTACAATGGCTTCAGCTACAGCACCCACCAACTCTCTCCCTCCTCCCCCACCCCGCCAAAATACCTCCACCCTCCATCAGCAACCCCATCTTCCTCCCGTGTCCACCTCTCCTCCTCATTCCTTGGCGCCCGTCCGCTCCGCCGACTAGGTTTCTCGGCTGCCGATCCCTTGCTGGCATTGCAGGTGGCGTCCAAGGTGAGGACAGTGAGCGGGAAAGGAGTGAGAGGTGTAGTGTCCATGGCGAAGAAGAGTGTGGGGGATTTGAGTGCGGCGGATTTGGAAGGAAAGAAGGTTTTCGTCAGGGCTGACTTGAATGTTCCTCTTGATGATAATCAGAATATTACTGATGATACTAGGATCCGTGCTGCTGTACCAACTATCAAATATCTTATTAAGAATGGCGCTAAAGTCATCCTTTCCAGTCACTTGGGACGGCCAAAGGGTGTCACTCCCAAATACAGCTTGGCACCTCTTGTTCCCCGGCTATCCGAACTCATCGGAATCGAGGTTGTTAAGGCCGATGACAGTATTGGTCCAGAAGTAGAAAAGTTGGTGGCTTCCCTTCCAAACGGAGGAGTTCTTCTTCTAGAAAATGTAAGGTTTTACAAGGAGGAAGAGAAGAACGATCCTGAGCATGCAAAAAAGCTCGCCGCACTAGCAGATCTGTATGTGAATGATGCTTTTGGTACTGCTCATAGGGCACATGCATCAACAGAGGGAGTCACTAAATACTTGAAGCCATCTGTTGCTGGTTTTCTCTTGCAAAAGGAACTTGACTACCTTGTTGGGGCAGTATCAACCCCCAAAAGGCCATTTGCTGCAATTGTGGGTGGTTCCAAGGTCTCATCTAAAATTGGTGTGATTGAGTCACTTCTAGAAAAAGTTGACATACTTCTTCTCGGTGGAGGGATGATCTTCACATTTTACAAGGCACAAGGTCTCTCAGTTGGTTCATCCCTTGTAGAGGAAGATAAGTTGGATCTTGCTACAACACTACTTGCAAAAGCCAAGGCAAAGGGGGTGTCACTCTTGTTACCGAGTGATGTCGTGATTGCAGACAAATTTGCTCCTGATGCAAACAGCCAGATTGTGCCAGCATCTGCCATTCCCGATGGTTGGATGGGATTGGATATTGGTCCAGATTCTATTAAAACATTCAACGAAGCATTGGACACCACCAAAACCATCATATGGAATGGACCAATGGGAGTGTTCGAGTTTGACAAGTTTGCTACTGGGACAGAGGCTATTGCAAAGAAGTTGGCTGACCTCAGTGGAAAAGGGGTGACAACTATTATTGGAGGAGGAGATTCCGTCGCTGCTGTGGAGAAAGTAGGAGTCGCTGAAGTCATGAGCCACATATCCACTGGTGGTGGTGCAAGTTTGGAGTTATTGGAAGGCAAAGAGCTTCCAGGTGTCCTTGCTCTCGACGAAGCCACACCAGTTGCTGTGTAAGACAGTTTTCAGTTTGTAATTTTTTTCCCACCCTCGGTGTCGAGTGTTCTTGCAACCTTGTAAAAGAGCTTATGTTGTAGGATCCTTCCGAGTTTGTTAGTGTAAATCTTATCATGTCTGAGTTATTATTCTCTTCGCACATAAATTGCTTGTAACTGTAAACCTAGGCTGAGAGCCATAAATTCAAATAAATCATCCATTAGTTGTTGAACAACCG
TARGET=15,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03254 Ps_contig_mira-and-tgicl-ass_franssen_29315
SEQUENCE=TAATCACTGCTTCAGCCACTCAAATTCTTCAGGGAGAATATCTTGTGATTGNNAAACAAAGATTAAAACAAAGATTTCATACAGAAGTCCGTCCGAATGACAGGTAAATTGGGAAAAAACGGTGAATCAACCATATTCAGTTTTACCTGCCTTGAGTGTGTCATCCATTCGCCACTCCTATAAGCACTTAGAAACTGCTCTCAGACGCATATTCATCTATGCTTGAGAAGCTTACAATTCATAAAATATACCAACTCTTTGTGTACAACTGAAGACAGAGACTACATGACTCTCCATCTCAAAATGCGAATTTGTATCCAAAAAAACAGTGGTTTACATAGTTCATCATCGTGTTTTCCCTTGGATTTTACAATTCCAAATATCGTAGCGGACAAAATGGATCTCGATTTTTCTTGCAACCATTAGGAGCTAGGACCAAGCTGACCAGTAGTTACATCAATCTTTATAGCACAGTCTATATCATACTTTTGCACCAAGGAAAGACTTTACACTTGCTGGAGAGTTTCCAGGCTTAGCAGTGGTTGTACTTCCAAAATTGATCTTTGTTTTTTTATCAGCTGGCTTTAATATTTGGAAAGAGCACATCAGTGTCTCATCAACACTCATCATAGCACCGGCATTGTCAAACTCTCCACAATAATTAGGTGCTGAAAATATTGTTACGAGTTGTCTATTAGCAAAGAACTCATATCCATCCTCCACAACCTGATGAGCACGGCAAATTAGGTCAAGATCCTGTTTCTGAAGAAACTCTGTGATCTTATCAGCACCAAATGTATATGAAACTCCCCTGTCATTCATTCCCCATCCTTGAACATCTTTGCTTGGGTCAGACCAAAGAAGATCACAAAGCAAACCTGTATCAGGAACATCAGTGGGCCGCTGTAGATTTCTAATCTGATCCAAACTATGCAAGTCAGGAGAAATCCCCCCATGCATACACAAAATCTTTTCATCAACAAGAGCTGCCACAGGAAGGCAATTGAAGCAGTCTGTAAATGTCTTCCATAATCTTACATTGAACCTTCTCTTGCACTCATCATAAAATCCATAAATTCTGTTTATAGAAGCACATTCATGATTTCCTCTCAATAGGAAAAAGTTCTCAGGATATCTAATCTTATAAGCAAGAAGAAGGCAAATTGTTTCTAAACTCTGCTTTCCCCGATCCACATAATCCCCCAAAAACAAGTAGTTAGCCTCGGGAGGCAATCCACCATACTCAAAAAAGCCTCAAGAGATCAGAATATTGACCATGTACATCACCACAAATTTTGATAGGTGCTTCGAGTTCTAATAAATAAGGTTGTTGAATGAAAATGTCTCTAGAAACGACGCAGAGTTGACGGATCTCGGCCTCCGATAGTTGAACTTGCTTCCCCGGACGGTTTCGAACTTCTAACAGTCGACTGATTATGTCGTCAACAACTGATTGCTCCATTTTCTTCTTCGCCGACTTCAAGTTAACGAATAGCAGACACAGTCAATTCGATTAGATGAAGAAGAAGAAGATGAAGATGAAGATGCGAAGGTTCGAAGTCAAAATCGGAAGACTAGAAGTGAAGGAGGCGTGGAAACGGAGGAGAAAACGACGACGGGATGTTGGGAGTGACAGTCGTTTTGGTGGTAGAGGTCCGTTGTTTTGGAATGGGTCTTAATTGGACACATTCACTTTCGGTCCCCGAC
TARGET=1520,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2585
PRIMER_LEFT_PENALTY=0.203971
PRIMER_RIGHT_PENALTY=0.054514
PRIMER_LEFT_SEQUENCE=GATTGCTCCATTTTCTTCTTCG
PRIMER_RIGHT_SEQUENCE=GTCCAATTAAGACCCATTCCAA
PRIMER_LEFT=1451,22
PRIMER_RIGHT=1684,22
PRIMER_LEFT_TM=60.204
PRIMER_RIGHT_TM=60.055
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.2834
PRIMER_LEFT_1_PENALTY=0.180765
PRIMER_RIGHT_1_PENALTY=0.102638
PRIMER_LEFT_1_SEQUENCE=TGTCTCTAGAAACGACGCAGAG
PRIMER_RIGHT_1_SEQUENCE=ACCTTCGCATCTTCATCTTCAT
PRIMER_LEFT_1=1341,22
PRIMER_RIGHT_1=1553,22
PRIMER_LEFT_1_TM=59.819
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.2922
PRIMER_LEFT_2_PENALTY=0.189596
PRIMER_RIGHT_2_PENALTY=0.102638
PRIMER_LEFT_2_SEQUENCE=TCTAGAAACGACGCAGAGTTGA
PRIMER_RIGHT_2_SEQUENCE=ACCTTCGCATCTTCATCTTCAT
PRIMER_LEFT_2=1345,22
PRIMER_RIGHT_2=1553,22
PRIMER_LEFT_2_TM=60.190
PRIMER_RIGHT_2_TM=60.103
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.3066
PRIMER_LEFT_3_PENALTY=0.203971
PRIMER_RIGHT_3_PENALTY=0.102638
PRIMER_LEFT_3_SEQUENCE=GATTGCTCCATTTTCTTCTTCG
PRIMER_RIGHT_3_SEQUENCE=ACCTTCGCATCTTCATCTTCAT
PRIMER_LEFT_3=1451,22
PRIMER_RIGHT_3=1553,22
PRIMER_LEFT_3_TM=60.204
PRIMER_RIGHT_3_TM=60.103
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=0.3192
PRIMER_LEFT_4_PENALTY=0.216588
PRIMER_RIGHT_4_PENALTY=0.102638
PRIMER_LEFT_4_SEQUENCE=CCTCCGATAGTTGAACTTGCTT
PRIMER_RIGHT_4_SEQUENCE=ACCTTCGCATCTTCATCTTCAT
PRIMER_LEFT_4=1377,22
PRIMER_RIGHT_4=1553,22
PRIMER_LEFT_4_TM=59.783
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_03255 Ps_contig_mira-and-tgicl-ass_franssen_29320
SEQUENCE=ACGTCTTTAACTATCAACAAGCGGTCATANNNATGTAAGTTTTTTTCATTAAAATAGATACATATCAAGATGAAGATAAGATGTACATCTCAAATCATTAAATAAAATTAAACCAAAATTCAACAAAAACCAATAACACATAAAATTAAAATGATCAATAATAATCATTGCACTGTCTCTTCCCTAGCCCATGAAACCCAAAACCAAAAACAAAGATCCCATCAAAATACCAACACCATTAGAAACACCGACAGCTCCACTATCCTTTGGTGGTGGTGGAGAAGTTGAGTTAGTCTCAGAAGATGGGGTTGATGGAGTGGTTGATGGTGTTGTTGATGGTGTTGTTGAAGGAGTAGGAGTTTTGGTTGTTGGAGGGGTGCCACTTGGAGTTGGGGTGGTGCTAGCAGCAACAACATTGACTTCAAGTTTCATTCCAGCAGTACAATGACCTGGTGTTGGACATATGAAGTAAATTTTTCCAGTCTTTTGTTAATGGAACCTTTGAATCTCCATCTGCATAATTCTTTATGGTGTTGCTTGAACTGCAACTTTTGTAATCACTCTCATCAACTTCATTCACTTGATGATTCCTCATTATTTAGAAACCTAACTTAAAATTTATCCAACCTAAACTTTAAG
TARGET=268,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1448
PRIMER_LEFT_PENALTY=0.086698
PRIMER_RIGHT_PENALTY=0.058146
PRIMER_LEFT_SEQUENCE=ATTGCACTGTCTCTTCCCTAGC
PRIMER_RIGHT_SEQUENCE=ACAACACCATCAACAACACCAT
PRIMER_LEFT=166,22
PRIMER_RIGHT=344,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=60.058
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.1828
PRIMER_LEFT_1_PENALTY=0.124655
PRIMER_RIGHT_1_PENALTY=0.058146
PRIMER_LEFT_1_SEQUENCE=TCATTGCACTGTCTCTTCCCTA
PRIMER_RIGHT_1_SEQUENCE=ACAACACCATCAACAACACCAT
PRIMER_LEFT_1=164,22
PRIMER_RIGHT_1=344,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.058
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=0.1972
PRIMER_LEFT_2_PENALTY=0.139083
PRIMER_RIGHT_2_PENALTY=0.058146
PRIMER_LEFT_2_SEQUENCE=ATCATTGCACTGTCTCTTCCCT
PRIMER_RIGHT_2_SEQUENCE=ACAACACCATCAACAACACCAT
PRIMER_LEFT_2=163,22
PRIMER_RIGHT_2=344,22
PRIMER_LEFT_2_TM=60.139
PRIMER_RIGHT_2_TM=60.058
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=182
PRIMER_PAIR_PENALTY_3=0.2381
PRIMER_LEFT_3_PENALTY=0.179943
PRIMER_RIGHT_3_PENALTY=0.058146
PRIMER_LEFT_3_SEQUENCE=ATCCCATCAAAATACCAACACC
PRIMER_RIGHT_3_SEQUENCE=ACAACACCATCAACAACACCAT
PRIMER_LEFT_3=215,22
PRIMER_RIGHT_3=344,22
PRIMER_LEFT_3_TM=59.820
PRIMER_RIGHT_3_TM=60.058
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=130
PRIMER_PAIR_PENALTY_4=0.2398
PRIMER_LEFT_4_PENALTY=0.179943
PRIMER_RIGHT_4_PENALTY=0.059875
PRIMER_LEFT_4_SEQUENCE=ATCCCATCAAAATACCAACACC
PRIMER_RIGHT_4_SEQUENCE=AGATGGAGATTCAAAGGTTCCA
PRIMER_LEFT_4=215,22
PRIMER_RIGHT_4=514,22
PRIMER_LEFT_4_TM=59.820
PRIMER_RIGHT_4_TM=59.940
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=300
=
PRIMER_SEQUENCE_ID=SSR_03256 Ps_contig_mira-and-tgicl-ass_franssen_29321
SEQUENCE=CGGGGAATGTATTAGGTAGAACCAAGCTGGCTTTATAGAAAATGTCAATAACCACTTCACTATATTATTTAAATTTAAACTCATCTCCAGTAGTTGGTGACATAGTAAACAAATGATTTAGTACTATATATATCCATAACTTCATTTGTCATTGGGACTAGAAACATTTTCTTACAAATATTGCATTTTGAGAACAAAGAGAGTTCATAGACATGAATACATCAATGGTGGCTTCTTTTTTAGTTTTGTTGTTGGCTTTTCCTTGTGTATTTGCAACTGATTACACTGTTGGTGATGCTAATCAATGGACTCAAGGGGTTGATTACACTACATGGACTTCTGGAAAAACATTCAAAGTTGGTGATAATTTAGTGTTCAAATATGGAGGAACTCATCAAGTGAATGAAGTTGATGAGAGTGATTACAAAAGTTGCAGTTCAAGCAACACCATAAAGAACTATGCAGATGGAGATTCAAAGGTTCCATTAACAAAAACAGGAAAAATTTACTTCATATGTCCAACACCAGGTCATTGTACTGCTGGAATGAAACTTGAAGTCAATGTTGTTGCTGCTAGCACCACCCCAACTCCAAGTGGCACCCCTCCAACAACCAAAACTCCTACTCCGTCAACAACACCATCAACAACACCATCAACCACTCCATCAACCCCATCTTCTGAGACAAATACAACTTCTCCACCACCACCAAAGGATAGTGGAGCTGTCGGTGTTTCTAATGGTGTTGGTATTTTGATGGGATCTTTGTTTTTGGCTTTGGGTTTCATGGGCTAGGGAAGAGACAGTGCAATGATTATTATTGATCTATTTTTAATTTTATGTTGTTANTTTGGTTTTTGTTTAGNTTAAAGTTTTTTAGGGTTTTTTAAATTTTTTTAATTTTAA
TARGET=699,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1198
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.086698
PRIMER_LEFT_SEQUENCE=ACCAAAACTCCTACTCCGTCAA
PRIMER_RIGHT_SEQUENCE=ATTGCACTGTCTCTTCCCTAGC
PRIMER_LEFT=611,22
PRIMER_RIGHT=810,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=200
PRIMER_PAIR_PENALTY_1=0.1198
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.086698
PRIMER_LEFT_1_SEQUENCE=AACCAAAACTCCTACTCCGTCA
PRIMER_RIGHT_1_SEQUENCE=ATTGCACTGTCTCTTCCCTAGC
PRIMER_LEFT_1=610,22
PRIMER_RIGHT_1=810,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.1198
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.086698
PRIMER_LEFT_2_SEQUENCE=CAACCAAAACTCCTACTCCGTC
PRIMER_RIGHT_2_SEQUENCE=ATTGCACTGTCTCTTCCCTAGC
PRIMER_LEFT_2=609,22
PRIMER_RIGHT_2=810,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.1198
PRIMER_LEFT_3_PENALTY=0.033137
PRIMER_RIGHT_3_PENALTY=0.086698
PRIMER_LEFT_3_SEQUENCE=CCAAAACTCCTACTCCGTCAAC
PRIMER_RIGHT_3_SEQUENCE=ATTGCACTGTCTCTTCCCTAGC
PRIMER_LEFT_3=612,22
PRIMER_RIGHT_3=810,22
PRIMER_LEFT_3_TM=60.033
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=199
PRIMER_PAIR_PENALTY_4=0.1448
PRIMER_LEFT_4_PENALTY=0.058146
PRIMER_RIGHT_4_PENALTY=0.086698
PRIMER_LEFT_4_SEQUENCE=ACAACACCATCAACAACACCAT
PRIMER_RIGHT_4_SEQUENCE=ATTGCACTGTCTCTTCCCTAGC
PRIMER_LEFT_4=632,22
PRIMER_RIGHT_4=810,22
PRIMER_LEFT_4_TM=60.058
PRIMER_RIGHT_4_TM=59.913
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_03257 Ps_contig_mira-and-tgicl-ass_franssen_29322
SEQUENCE=TTCTTTTGTAGAACAAAGAAAGTTTAATAAANAATGAACACATCAATGTATTGCTTCTTTTTTTGGTTTTTGTTTGTTGGCTTTTCCTTTATGTTATTTGCAACTGATTACACTGTTGGTGATGCTAATCAATGGACTCAAGGGGTTGATTACACGACATGGACTTCTGGAAAAACATTCAAAGTTGGTGATAATTTAGTGTTCAAATATGGAGGAACTCATCAAGTGAATGAAGTTGATGAGAGTGATTACAAAAGTTGCAGTTCAAGCAACACCATAAAGAACTATGCAGATGGAGATTCAAAGGTTCCATTAACAAAAACAGGAAAGATTTACTTCATATGTCCAACACCAGGTCATTGTACTGCTGGAATGAAACTTGAAGTCAATGTTGTTGCTGCTAGCACCACCCCAACTCCTAGTGGCACCCCTCCAACAACCAAAACTCCTACTCCTTCAACAACACCATCAACCACTCCATCAACCCCATCTTCTGAGACTAACTCAACTTCTCCACCACCACCAAAGGATAGTGGAGCTATCGGTGTTTCTAATGGTGTTGGTATTTTGATGGGATCTTTGTTTTTGGTTTTGGGTTTCATGGGCTAGGGAAGAGGCGGTGCAATGATTGTTATTGATCATTTTAATTTATGTGTTATTGGGTTTTTGTTGAATTTATACAACACATTATTGTTATCCATCATTCAATTAAAATTTACTAAA
TARGET=514,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2117
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.179943
PRIMER_LEFT_SEQUENCE=ATGCAGATGGAGATTCAAAGGT
PRIMER_RIGHT_SEQUENCE=ATCCCATCAAAATACCAACACC
PRIMER_LEFT=286,22
PRIMER_RIGHT=576,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=59.820
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.2398
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=0.179943
PRIMER_LEFT_1_SEQUENCE=CAGATGGAGATTCAAAGGTTCC
PRIMER_RIGHT_1_SEQUENCE=ATCCCATCAAAATACCAACACC
PRIMER_LEFT_1=289,22
PRIMER_RIGHT_1=576,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=59.820
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.2398
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.179943
PRIMER_LEFT_2_SEQUENCE=AGATGGAGATTCAAAGGTTCCA
PRIMER_RIGHT_2_SEQUENCE=ATCCCATCAAAATACCAACACC
PRIMER_LEFT_2=290,22
PRIMER_RIGHT_2=576,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=59.820
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.2614
PRIMER_LEFT_3_PENALTY=0.081468
PRIMER_RIGHT_3_PENALTY=0.179943
PRIMER_LEFT_3_SEQUENCE=GCAGATGGAGATTCAAAGGTTC
PRIMER_RIGHT_3_SEQUENCE=ATCCCATCAAAATACCAACACC
PRIMER_LEFT_3=288,22
PRIMER_RIGHT_3=576,22
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=59.820
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=0.4628
PRIMER_LEFT_4_PENALTY=0.282868
PRIMER_RIGHT_4_PENALTY=0.179943
PRIMER_LEFT_4_SEQUENCE=TTCATATGTCCAACACCAGGTC
PRIMER_RIGHT_4_SEQUENCE=ATCCCATCAAAATACCAACACC
PRIMER_LEFT_4=336,22
PRIMER_RIGHT_4=576,22
PRIMER_LEFT_4_TM=59.717
PRIMER_RIGHT_4_TM=59.820
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_03258 Ps_contig_mira-and-tgicl-ass_franssen_29336
SEQUENCE=GGCAACGAATAACATGATTGTAATAATTTAACTGCTAAAACAGTTCCTTCTGTTATGTTTTCGTGAATCTTCACAGAGAAAGTAAATTAGAGAGAGAGTAGAAAAGCTTGAAGCTGTATAAGAGACATCAGTGTCTCTAGTAGAGTGTGTTTGTCTTGTGAGAGAGAGAAAGTAAAGTGAATAGTTGGTGAAGATGATGAATAACTCAGTAGTTTCAGACAACAGTTTTATTATTGAAAGTGAAGAAGAAGATGATAAGGATTTTAACGATGGAAATGTTTCTGATTCTTCTAATTACTCGAATGAAAATCCGCCACAGAGGAAACCAAGTTCTTATAACCCTTCATGGCCACAGAGTTACAGGCAATCAATTGATCTATACAGTAGTGTACCGTCGCCCAATATTGGATTTCTGGGAACTTCATCAGTTTCAAGATTAAGCAGTTCCTTCCTTTCTACATCTCTAACAAGGAGGCACACTCCAGAATCACTTCCTTCCGCTGCAAAGCCTCTGATACAACAGCAGAATGAAGATGAGCCGCAACAAAGACGGAGCTCTCACGCCTTGCTTCCGCCTTCTGCTAGAAGGTCGTCTAGTCTTAAGAAAGACTCTAAGGTTTCTGATGAAGTTCCTTCTGGCCATTGCTCTTTCGGACAGGCTGTGCTTAATGGCATAAATGTTCTTTGTGGAGTAGGGATCCTTTCAACCCCTTATGCTGCTAAAGAAGGTGGATGGGTAGGACTTTCCATCTTGTTCATTTTCGGAATTCTTTCCTTTTATACCGGATTGCTCCTGCGTTCTTGCTTGGACAGTGAACCTGGTCTTGAGACATATCCTGACATTGGCCAAGCTGCTTTTGGTACTGCCGGACGTATTGCCATTTCAATAGTATTGTACATCGAATTATATGGCTGTTGCATTGAGTATATAATTTTGGAAGGCGACAACTTGGCTTCGTTGTTTCCAAATGCATACTTAAATTTAGGTGTAATTGAGTTGAATCCGAGAACACTCTTTGCTGTGATTGCCACCTTGGCTGTTCTTCCTACCGTTTGGCTCCGCGACCTTAGCATTCTTAGTTACATTTCAGCCGGTGGAGTTATCGCATCCGTTTTAGTAGTTCTGTGCTTGTTATGGGTCGGTGTTGAAGACGTTGGCTTTCAGCGCTCGGGGACAACACTCAATTTTGCAACTCTACCTGTTGCTATCGGTCTTTACGGCTACTGTTATTCTGGACATGCTGTGTTCCCAAACATTTACACATCCATGGCAAAATCCAAAACCAATTCCCTGCAGTACTAGTAGCATGTTTCGGAAGTTTAAGTAGCTTACTGTATGCTGGAGGCG
TARGET=89,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9969
PRIMER_LEFT_PENALTY=2.792901
PRIMER_RIGHT_PENALTY=0.203971
PRIMER_LEFT_SEQUENCE=TCGTGAATCTTCACAGAGAAAG
PRIMER_RIGHT_SEQUENCE=GTGGCCATGAAGGGTTATAAGA
PRIMER_LEFT=60,22
PRIMER_RIGHT=352,22
PRIMER_LEFT_TM=57.207
PRIMER_RIGHT_TM=60.204
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=3.4568
PRIMER_LEFT_1_PENALTY=2.792901
PRIMER_RIGHT_1_PENALTY=0.663948
PRIMER_LEFT_1_SEQUENCE=TCGTGAATCTTCACAGAGAAAG
PRIMER_RIGHT_1_SEQUENCE=TGGCCATGAAGGGTTATAAGAA
PRIMER_LEFT_1=60,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=57.207
PRIMER_RIGHT_1_TM=60.664
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=4.0283
PRIMER_LEFT_2_PENALTY=2.792901
PRIMER_RIGHT_2_PENALTY=1.235354
PRIMER_LEFT_2_SEQUENCE=TCGTGAATCTTCACAGAGAAAG
PRIMER_RIGHT_2_SEQUENCE=GGCCATGAAGGGTTATAAGAACT
PRIMER_LEFT_2=60,22
PRIMER_RIGHT_2=350,23
PRIMER_LEFT_2_TM=57.207
PRIMER_RIGHT_2_TM=59.765
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=4.2538
PRIMER_LEFT_3_PENALTY=2.792901
PRIMER_RIGHT_3_PENALTY=1.460885
PRIMER_LEFT_3_SEQUENCE=TCGTGAATCTTCACAGAGAAAG
PRIMER_RIGHT_3_SEQUENCE=GGCGGATTTTCATTCGAGTAAT
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=57.207
PRIMER_RIGHT_3_TM=61.461
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=4.3731
PRIMER_LEFT_4_PENALTY=2.792901
PRIMER_RIGHT_4_PENALTY=1.580186
PRIMER_LEFT_4_SEQUENCE=TCGTGAATCTTCACAGAGAAAG
PRIMER_RIGHT_4_SEQUENCE=TGGCCATGAAGGGTTATAAGA
PRIMER_LEFT_4=60,22
PRIMER_RIGHT_4=351,21
PRIMER_LEFT_4_TM=57.207
PRIMER_RIGHT_4_TM=59.420
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_03259 Ps_contig_mira-and-tgicl-ass_franssen_29336
SEQUENCE=GGCAACGAATAACATGATTGTAATAATTTAACTGCTAAAACAGTTCCTTCTGTTATGTTTTCGTGAATCTTCACAGAGAAAGTAAATTAGAGAGAGAGTAGAAAAGCTTGAAGCTGTATAAGAGACATCAGTGTCTCTAGTAGAGTGTGTTTGTCTTGTGAGAGAGAGAAAGTAAAGTGAATAGTTGGTGAAGATGATGAATAACTCAGTAGTTTCAGACAACAGTTTTATTATTGAAAGTGAAGAAGAAGATGATAAGGATTTTAACGATGGAAATGTTTCTGATTCTTCTAATTACTCGAATGAAAATCCGCCACAGAGGAAACCAAGTTCTTATAACCCTTCATGGCCACAGAGTTACAGGCAATCAATTGATCTATACAGTAGTGTACCGTCGCCCAATATTGGATTTCTGGGAACTTCATCAGTTTCAAGATTAAGCAGTTCCTTCCTTTCTACATCTCTAACAAGGAGGCACACTCCAGAATCACTTCCTTCCGCTGCAAAGCCTCTGATACAACAGCAGAATGAAGATGAGCCGCAACAAAGACGGAGCTCTCACGCCTTGCTTCCGCCTTCTGCTAGAAGGTCGTCTAGTCTTAAGAAAGACTCTAAGGTTTCTGATGAAGTTCCTTCTGGCCATTGCTCTTTCGGACAGGCTGTGCTTAATGGCATAAATGTTCTTTGTGGAGTAGGGATCCTTTCAACCCCTTATGCTGCTAAAGAAGGTGGATGGGTAGGACTTTCCATCTTGTTCATTTTCGGAATTCTTTCCTTTTATACCGGATTGCTCCTGCGTTCTTGCTTGGACAGTGAACCTGGTCTTGAGACATATCCTGACATTGGCCAAGCTGCTTTTGGTACTGCCGGACGTATTGCCATTTCAATAGTATTGTACATCGAATTATATGGCTGTTGCATTGAGTATATAATTTTGGAAGGCGACAACTTGGCTTCGTTGTTTCCAAATGCATACTTAAATTTAGGTGTAATTGAGTTGAATCCGAGAACACTCTTTGCTGTGATTGCCACCTTGGCTGTTCTTCCTACCGTTTGGCTCCGCGACCTTAGCATTCTTAGTTACATTTCAGCCGGTGGAGTTATCGCATCCGTTTTAGTAGTTCTGTGCTTGTTATGGGTCGGTGTTGAAGACGTTGGCTTTCAGCGCTCGGGGACAACACTCAATTTTGCAACTCTACCTGTTGCTATCGGTCTTTACGGCTACTGTTATTCTGGACATGCTGTGTTCCCAAACATTTACACATCCATGGCAAAATCCAAAACCAATTCCCTGCAGTACTAGTAGCATGTTTCGGAAGTTTAAGTAGCTTACTGTATGCTGGAGGCG
TARGET=160,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9969
PRIMER_LEFT_PENALTY=2.792901
PRIMER_RIGHT_PENALTY=0.203971
PRIMER_LEFT_SEQUENCE=TCGTGAATCTTCACAGAGAAAG
PRIMER_RIGHT_SEQUENCE=GTGGCCATGAAGGGTTATAAGA
PRIMER_LEFT=60,22
PRIMER_RIGHT=352,22
PRIMER_LEFT_TM=57.207
PRIMER_RIGHT_TM=60.204
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=3.4568
PRIMER_LEFT_1_PENALTY=2.792901
PRIMER_RIGHT_1_PENALTY=0.663948
PRIMER_LEFT_1_SEQUENCE=TCGTGAATCTTCACAGAGAAAG
PRIMER_RIGHT_1_SEQUENCE=TGGCCATGAAGGGTTATAAGAA
PRIMER_LEFT_1=60,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=57.207
PRIMER_RIGHT_1_TM=60.664
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=4.0283
PRIMER_LEFT_2_PENALTY=2.792901
PRIMER_RIGHT_2_PENALTY=1.235354
PRIMER_LEFT_2_SEQUENCE=TCGTGAATCTTCACAGAGAAAG
PRIMER_RIGHT_2_SEQUENCE=GGCCATGAAGGGTTATAAGAACT
PRIMER_LEFT_2=60,22
PRIMER_RIGHT_2=350,23
PRIMER_LEFT_2_TM=57.207
PRIMER_RIGHT_2_TM=59.765
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=4.2538
PRIMER_LEFT_3_PENALTY=2.792901
PRIMER_RIGHT_3_PENALTY=1.460885
PRIMER_LEFT_3_SEQUENCE=TCGTGAATCTTCACAGAGAAAG
PRIMER_RIGHT_3_SEQUENCE=GGCGGATTTTCATTCGAGTAAT
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=57.207
PRIMER_RIGHT_3_TM=61.461
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=4.3731
PRIMER_LEFT_4_PENALTY=2.792901
PRIMER_RIGHT_4_PENALTY=1.580186
PRIMER_LEFT_4_SEQUENCE=TCGTGAATCTTCACAGAGAAAG
PRIMER_RIGHT_4_SEQUENCE=TGGCCATGAAGGGTTATAAGA
PRIMER_LEFT_4=60,22
PRIMER_RIGHT_4=351,21
PRIMER_LEFT_4_TM=57.207
PRIMER_RIGHT_4_TM=59.420
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_03260 Ps_contig_mira-and-tgicl-ass_franssen_29340
SEQUENCE=TTTGAGTAAACAATGGGACGTATGATAAAATTAACCCCGTAATTTTGAAGTGCAATTCAATACATTTGAGACGCGACTTTTAAACAAATCATGCTAAGTTTCACAAACAAAATAAAAATAAACTAATTAGTAATGAAAATTAAATAAAAACGGATGATAATTATACACTTTTAACACCTCCCTTCATCTCCACGCCATTGTCAGCATGTTCAACAAATCTTGACCAGTATGGATGTGATTTCCATACTCTATCCATCTCTTCAATTGGTATTCCCTTTGTTTCAGGAAGCATGGCGAATATGTAAATTGTCATCACCACAACAAAGAACGCAAAGAAGAGGAACAATCCAAACTTCATGTGACAAAGCATGGTCAGGAAAATCTGTGCGACTAAGAAGGTGAAGAGCATGTTGACCGATACGTTGACACTCTGCGCGGCGGAACGAATTTCCAATGGAAAAATCTCACTAGGCACCAACCAACCAAGAGGACCCCATGACCAAGCAAATCCGGCTACATAAATGCATATGAAAAGCACAACTACTATAGCATACCATTTTGGTAAATCACCAGGTTCTCCACTTGTTCCGAACTTAGCCGCAATTGAAATTGCAACTGCAACCTGACAAATAAGCATTTGAACACCACCTTCAAGGAAAAGAGCTCTCCTACCCCATTTATCAACTCCATAAATCGAGACACAAGTAGCAACAACGTTAACAACACCGGTGATAACAGCTGACATGAGCGAAGCATCATCCTTAAACCCAATTGAGCTAAACAACACCGGCGCGTAAAACATAATAACATTAATCCCAGTAAACTGTTGGAAAAACGGAATCAACACAGCCATACTAAGCTGAGGTCTATATTTCCTCTGCAACAAATTCCTCCAAGGATTCTCAACCTGCATCGACGTTTCACTAGCAGCCACAAGATCATTAAACTCTTCGTCAACATCCTCAATACCGCGAATTCTCCTTAGTTGAGCCTTGGCACCATCGCGATTGCCACGTTCAATCATCGAATTAGGCGTGTCGGGAAGGATTAGCGAACCAATGGTTATAATAAGCGCAGGGACCATAGCACCACCCAAACTCAATCTCCATCCCCATCCACCTTCGATTTTTGCAAAAAAGTAGTTCAACACATTGGCCACTAGTATACCAATTGTAATTGATAATTGAAACCCAATATTCAATGCTCCTCTGTACTTGTATGGAGCCATCTCAGACAAATAAAGTGGCACAGACTGATTAGCAAACCCGATACCAAACCCGAGCAAGATCCGACCCACGATCAACATAGCAACGTTTTGAGCGAAGCCATTAATGAGAGCACCGACAAGAAAAAGCAAACCTCCGAAAAGCATGGAAAGTTTCCGGCCAAATCTTCTGGTGATCGTGGAGGCCACCACCGACGACAAAAGCGCCGCCAGGTACAACGAGGAAGTAAACAACGTCAGTGCCTCACTATCATATTGACAGTACTGGTTCTTCGAATGTTGCGCGTTTTTCTTCCGGTACACCGCCGGAAAAAACTTCTCAAGAAATGGATTCATCGACGTAACACCACCAGAAATTCCTATATCGTAGCCGAAGATTAAACCACCCATGGCTGCGACAACACATGTTATGGTGACGAACGGAGTTAGGTTTCCCGGGTACTCCTTGTTCCCCGCTCCGATGGGGATTCCGGCGGCAGGCATTTTTTTTCTCCGATGAAGAGAAGAAGAAGAAGAATAGTGAAAGAAAGAAAAAGAGTAACAATGAAAAAGTCAGCTCTGCGTTGNA
TARGET=474,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0165
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=AAGCATGGTCAGGAAAATCTGT
PRIMER_RIGHT_SEQUENCE=CTTTTCCTTGAAGGTGGTGTTC
PRIMER_LEFT=364,22
PRIMER_RIGHT=660,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.0165
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=AAGCATGGTCAGGAAAATCTGT
PRIMER_RIGHT_1_SEQUENCE=TCTTTTCCTTGAAGGTGGTGTT
PRIMER_LEFT_1=364,22
PRIMER_RIGHT_1=661,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.0242
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.019805
PRIMER_LEFT_2_SEQUENCE=AAGCATGGTCAGGAAAATCTGT
PRIMER_RIGHT_2_SEQUENCE=ACCTGGTGATTTACCAAAATGG
PRIMER_LEFT_2=364,22
PRIMER_RIGHT_2=574,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=59.980
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.0571
PRIMER_LEFT_3_PENALTY=0.037339
PRIMER_RIGHT_3_PENALTY=0.019805
PRIMER_LEFT_3_SEQUENCE=AACTTCATGTGACAAAGCATGG
PRIMER_RIGHT_3_SEQUENCE=ACCTGGTGATTTACCAAAATGG
PRIMER_LEFT_3=350,22
PRIMER_RIGHT_3=574,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=59.980
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=0.0638
PRIMER_LEFT_4_PENALTY=0.043988
PRIMER_RIGHT_4_PENALTY=0.019805
PRIMER_LEFT_4_SEQUENCE=CACAACAAAGAACGCAAAGAAG
PRIMER_RIGHT_4_SEQUENCE=ACCTGGTGATTTACCAAAATGG
PRIMER_LEFT_4=316,22
PRIMER_RIGHT_4=574,22
PRIMER_LEFT_4_TM=59.956
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=259
=
PRIMER_SEQUENCE_ID=SSR_03261 Ps_contig_mira-and-tgicl-ass_franssen_29340
SEQUENCE=TTTGAGTAAACAATGGGACGTATGATAAAATTAACCCCGTAATTTTGAAGTGCAATTCAATACATTTGAGACGCGACTTTTAAACAAATCATGCTAAGTTTCACAAACAAAATAAAAATAAACTAATTAGTAATGAAAATTAAATAAAAACGGATGATAATTATACACTTTTAACACCTCCCTTCATCTCCACGCCATTGTCAGCATGTTCAACAAATCTTGACCAGTATGGATGTGATTTCCATACTCTATCCATCTCTTCAATTGGTATTCCCTTTGTTTCAGGAAGCATGGCGAATATGTAAATTGTCATCACCACAACAAAGAACGCAAAGAAGAGGAACAATCCAAACTTCATGTGACAAAGCATGGTCAGGAAAATCTGTGCGACTAAGAAGGTGAAGAGCATGTTGACCGATACGTTGACACTCTGCGCGGCGGAACGAATTTCCAATGGAAAAATCTCACTAGGCACCAACCAACCAAGAGGACCCCATGACCAAGCAAATCCGGCTACATAAATGCATATGAAAAGCACAACTACTATAGCATACCATTTTGGTAAATCACCAGGTTCTCCACTTGTTCCGAACTTAGCCGCAATTGAAATTGCAACTGCAACCTGACAAATAAGCATTTGAACACCACCTTCAAGGAAAAGAGCTCTCCTACCCCATTTATCAACTCCATAAATCGAGACACAAGTAGCAACAACGTTAACAACACCGGTGATAACAGCTGACATGAGCGAAGCATCATCCTTAAACCCAATTGAGCTAAACAACACCGGCGCGTAAAACATAATAACATTAATCCCAGTAAACTGTTGGAAAAACGGAATCAACACAGCCATACTAAGCTGAGGTCTATATTTCCTCTGCAACAAATTCCTCCAAGGATTCTCAACCTGCATCGACGTTTCACTAGCAGCCACAAGATCATTAAACTCTTCGTCAACATCCTCAATACCGCGAATTCTCCTTAGTTGAGCCTTGGCACCATCGCGATTGCCACGTTCAATCATCGAATTAGGCGTGTCGGGAAGGATTAGCGAACCAATGGTTATAATAAGCGCAGGGACCATAGCACCACCCAAACTCAATCTCCATCCCCATCCACCTTCGATTTTTGCAAAAAAGTAGTTCAACACATTGGCCACTAGTATACCAATTGTAATTGATAATTGAAACCCAATATTCAATGCTCCTCTGTACTTGTATGGAGCCATCTCAGACAAATAAAGTGGCACAGACTGATTAGCAAACCCGATACCAAACCCGAGCAAGATCCGACCCACGATCAACATAGCAACGTTTTGAGCGAAGCCATTAATGAGAGCACCGACAAGAAAAAGCAAACCTCCGAAAAGCATGGAAAGTTTCCGGCCAAATCTTCTGGTGATCGTGGAGGCCACCACCGACGACAAAAGCGCCGCCAGGTACAACGAGGAAGTAAACAACGTCAGTGCCTCACTATCATATTGACAGTACTGGTTCTTCGAATGTTGCGCGTTTTTCTTCCGGTACACCGCCGGAAAAAACTTCTCAAGAAATGGATTCATCGACGTAACACCACCAGAAATTCCTATATCGTAGCCGAAGATTAAACCACCCATGGCTGCGACAACACATGTTATGGTGACGAACGGAGTTAGGTTTCCCGGGTACTCCTTGTTCCCCGCTCCGATGGGGATTCCGGCGGCAGGCATTTTTTTTCTCCGATGAAGAGAAGAAGAAGAAGAATAGTGAAAGAAAGAAAAAGAGTAACAATGAAAAAGTCAGCTCTGCGTTGNA
TARGET=1727,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4426
PRIMER_LEFT_PENALTY=0.010077
PRIMER_RIGHT_PENALTY=0.432475
PRIMER_LEFT_SEQUENCE=ATCGTAGCCGAAGATTAAACCA
PRIMER_RIGHT_SEQUENCE=ACGCAGAGCTGACTTTTTCATT
PRIMER_LEFT=1588,22
PRIMER_RIGHT=1788,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.432
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=201
PRIMER_PAIR_PENALTY_1=0.4426
PRIMER_LEFT_1_PENALTY=0.010077
PRIMER_RIGHT_1_PENALTY=0.432475
PRIMER_LEFT_1_SEQUENCE=ATCGTAGCCGAAGATTAAACCA
PRIMER_RIGHT_1_SEQUENCE=AACGCAGAGCTGACTTTTTCAT
PRIMER_LEFT_1=1588,22
PRIMER_RIGHT_1=1789,22
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=60.432
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.4724
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.432475
PRIMER_LEFT_2_SEQUENCE=GTGACGAACGGAGTTAGGTTTC
PRIMER_RIGHT_2_SEQUENCE=ACGCAGAGCTGACTTTTTCATT
PRIMER_LEFT_2=1637,22
PRIMER_RIGHT_2=1788,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=60.432
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.4724
PRIMER_LEFT_3_PENALTY=0.039879
PRIMER_RIGHT_3_PENALTY=0.432475
PRIMER_LEFT_3_SEQUENCE=GTGACGAACGGAGTTAGGTTTC
PRIMER_RIGHT_3_SEQUENCE=AACGCAGAGCTGACTTTTTCAT
PRIMER_LEFT_3=1637,22
PRIMER_RIGHT_3=1789,22
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=60.432
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=153
PRIMER_PAIR_PENALTY_4=0.9218
PRIMER_LEFT_4_PENALTY=0.489375
PRIMER_RIGHT_4_PENALTY=0.432475
PRIMER_LEFT_4_SEQUENCE=TCGTAGCCGAAGATTAAACCAC
PRIMER_RIGHT_4_SEQUENCE=ACGCAGAGCTGACTTTTTCATT
PRIMER_LEFT_4=1589,22
PRIMER_RIGHT_4=1788,22
PRIMER_LEFT_4_TM=60.489
PRIMER_RIGHT_4_TM=60.432
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_03262 Ps_contig_mira-and-tgicl-ass_franssen_29340
SEQUENCE=TTTGAGTAAACAATGGGACGTATGATAAAATTAACCCCGTAATTTTGAAGTGCAATTCAATACATTTGAGACGCGACTTTTAAACAAATCATGCTAAGTTTCACAAACAAAATAAAAATAAACTAATTAGTAATGAAAATTAAATAAAAACGGATGATAATTATACACTTTTAACACCTCCCTTCATCTCCACGCCATTGTCAGCATGTTCAACAAATCTTGACCAGTATGGATGTGATTTCCATACTCTATCCATCTCTTCAATTGGTATTCCCTTTGTTTCAGGAAGCATGGCGAATATGTAAATTGTCATCACCACAACAAAGAACGCAAAGAAGAGGAACAATCCAAACTTCATGTGACAAAGCATGGTCAGGAAAATCTGTGCGACTAAGAAGGTGAAGAGCATGTTGACCGATACGTTGACACTCTGCGCGGCGGAACGAATTTCCAATGGAAAAATCTCACTAGGCACCAACCAACCAAGAGGACCCCATGACCAAGCAAATCCGGCTACATAAATGCATATGAAAAGCACAACTACTATAGCATACCATTTTGGTAAATCACCAGGTTCTCCACTTGTTCCGAACTTAGCCGCAATTGAAATTGCAACTGCAACCTGACAAATAAGCATTTGAACACCACCTTCAAGGAAAAGAGCTCTCCTACCCCATTTATCAACTCCATAAATCGAGACACAAGTAGCAACAACGTTAACAACACCGGTGATAACAGCTGACATGAGCGAAGCATCATCCTTAAACCCAATTGAGCTAAACAACACCGGCGCGTAAAACATAATAACATTAATCCCAGTAAACTGTTGGAAAAACGGAATCAACACAGCCATACTAAGCTGAGGTCTATATTTCCTCTGCAACAAATTCCTCCAAGGATTCTCAACCTGCATCGACGTTTCACTAGCAGCCACAAGATCATTAAACTCTTCGTCAACATCCTCAATACCGCGAATTCTCCTTAGTTGAGCCTTGGCACCATCGCGATTGCCACGTTCAATCATCGAATTAGGCGTGTCGGGAAGGATTAGCGAACCAATGGTTATAATAAGCGCAGGGACCATAGCACCACCCAAACTCAATCTCCATCCCCATCCACCTTCGATTTTTGCAAAAAAGTAGTTCAACACATTGGCCACTAGTATACCAATTGTAATTGATAATTGAAACCCAATATTCAATGCTCCTCTGTACTTGTATGGAGCCATCTCAGACAAATAAAGTGGCACAGACTGATTAGCAAACCCGATACCAAACCCGAGCAAGATCCGACCCACGATCAACATAGCAACGTTTTGAGCGAAGCCATTAATGAGAGCACCGACAAGAAAAAGCAAACCTCCGAAAAGCATGGAAAGTTTCCGGCCAAATCTTCTGGTGATCGTGGAGGCCACCACCGACGACAAAAGCGCCGCCAGGTACAACGAGGAAGTAAACAACGTCAGTGCCTCACTATCATATTGACAGTACTGGTTCTTCGAATGTTGCGCGTTTTTCTTCCGGTACACCGCCGGAAAAAACTTCTCAAGAAATGGATTCATCGACGTAACACCACCAGAAATTCCTATATCGTAGCCGAAGATTAAACCACCCATGGCTGCGACAACACATGTTATGGTGACGAACGGAGTTAGGTTTCCCGGGTACTCCTTGTTCCCCGCTCCGATGGGGATTCCGGCGGCAGGCATTTTTTTTCTCCGATGAAGAGAAGAAGAAGAAGAATAGTGAAAGAAAGAAAAAGAGTAACAATGAAAAAGTCAGCTCTGCGTTGNA
TARGET=1747,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4426
PRIMER_LEFT_PENALTY=0.010077
PRIMER_RIGHT_PENALTY=0.432475
PRIMER_LEFT_SEQUENCE=ATCGTAGCCGAAGATTAAACCA
PRIMER_RIGHT_SEQUENCE=ACGCAGAGCTGACTTTTTCATT
PRIMER_LEFT=1588,22
PRIMER_RIGHT=1788,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.432
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=201
PRIMER_PAIR_PENALTY_1=0.4426
PRIMER_LEFT_1_PENALTY=0.010077
PRIMER_RIGHT_1_PENALTY=0.432475
PRIMER_LEFT_1_SEQUENCE=ATCGTAGCCGAAGATTAAACCA
PRIMER_RIGHT_1_SEQUENCE=AACGCAGAGCTGACTTTTTCAT
PRIMER_LEFT_1=1588,22
PRIMER_RIGHT_1=1789,22
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=60.432
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.4724
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.432475
PRIMER_LEFT_2_SEQUENCE=GTGACGAACGGAGTTAGGTTTC
PRIMER_RIGHT_2_SEQUENCE=ACGCAGAGCTGACTTTTTCATT
PRIMER_LEFT_2=1637,22
PRIMER_RIGHT_2=1788,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=60.432
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.4724
PRIMER_LEFT_3_PENALTY=0.039879
PRIMER_RIGHT_3_PENALTY=0.432475
PRIMER_LEFT_3_SEQUENCE=GTGACGAACGGAGTTAGGTTTC
PRIMER_RIGHT_3_SEQUENCE=AACGCAGAGCTGACTTTTTCAT
PRIMER_LEFT_3=1637,22
PRIMER_RIGHT_3=1789,22
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=60.432
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=153
PRIMER_PAIR_PENALTY_4=0.9218
PRIMER_LEFT_4_PENALTY=0.489375
PRIMER_RIGHT_4_PENALTY=0.432475
PRIMER_LEFT_4_SEQUENCE=TCGTAGCCGAAGATTAAACCAC
PRIMER_RIGHT_4_SEQUENCE=ACGCAGAGCTGACTTTTTCATT
PRIMER_LEFT_4=1589,22
PRIMER_RIGHT_4=1788,22
PRIMER_LEFT_4_TM=60.489
PRIMER_RIGHT_4_TM=60.432
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_03263 Ps_contig_mira-and-tgicl-ass_franssen_29346
SEQUENCE=ATGGTANNAGNAGAGTATCGGGAGATAGATAGATATTGTGAATGAAGAGGAAGTATAGTGTAGGACAGTGGATACTACATTTTATCCGAATAACTTCTCCCACTCTCTTTGCTTGAGATTCATTTCAATGGCGTCCACTCTCTTCTCCACTGCGCCACTCTGTTCTTCTTCCTCTTCTTCTATGTTCTCCACTCCGCTTAAATTATCCTTAAAGCCCACTGGTTTTTCTATCATTCCTTCCCTTGAAACCAAAAACCCCTTTTCTCTTCCCATTTCTCGAACTACAAGACGCTTCAAAATCACTGCCATGGCTCCCCCCAAAAAGCCTGCTGGTGGCAAGGCAAAGAAAGTTGTTGGAGTGATAAAATTGGCGCTCGAAGCCGGAAAGGCAACGCCGGCTCCACCGGTTGGACCGGCTCTTGGTTCAAAGGGTGTTAATATTATGGCTTTTTGTAAGGATTACAATGCCAGAACCGCTGATAAACCCGGTTATGTCATTCCCGTTGAAATCACTGTCTTTGATGACAAAAGTTTTACTTTCATATTGAAAACCCCACCTGCTTCTGTTTTACTGCTTAAGGCTGCTGGAGTGGAAAAGGGTTCGAAAGATCCCAAGGCACAAAAAGTAGGAAAAGTCACAATTGATCAGTTGCGGACAATTGCTTCTGAGAAGTTGCCGGACTTGAATTGCTCGACTATCGAATCAGCTATGAGAATTATAGCAGGCACTGCAGCAAATATGGGAATTGATGTTGATCCTCCTGTTCTTGAAGTCAAACAGAAGCAACTTTTGTAATGATTTTTTTTCAATTTTCCCTCTAAGTGTAAGGAAGTCCTTGCGGTAATAGATCACACAGGACATTTCATTGTACAATGTTTTGTCTTCTAAATCAGCAAAGAGAACATTTAATTTTAACTATTTCGGATTAAGTGTATTTATATAAATTATCAGTCATTTATATCAGTCACAAATTTGATGTTTGGTGTGATCTTGTTCTGTTTAATTCTAATTAAGAATTTTATTTGATGTGTGCTGAC
TARGET=23,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03264 Ps_contig_mira-and-tgicl-ass_franssen_29348
SEQUENCE=ATCTAAAAATCAAAACCCTATTTTCGACACAAATCCANATTAGCACAAACCATATAAATCTCTTCTTCCATTCATTTCAAATACCTCCAACCCCAAACCCTTTCTCCCCTTGTATGTTACACCATGAAGATTTTCAACCCTTCTAAATCCTTTCTTCAAAACGTCGTCGCTCCTTTCGACACCATTTTCATCAAAAACATGAAGCTCAACACCCTCGGTGCTTCCCACGTTAAACAATTCCATTTCCGCAGCAACAACTGATCGATCCTTTTACCAAAATACCCCTCCCCCTAAAACCTCTTTCCACCCTCCATTTCCACCCTCCATTTCCACCCTCCATTTCTTTCTTTCGAAATGGATCGTATCAATATTATCAAGAAAAGAACCATTCTGTTTAACAAATACGAGATAGGTAAAACGCTAGGTCAAGGAAATTTCGCCAAAGTGTTTCATGGAAGAAACGTTAGCACCAACGAGAACGTTGCTATAAAAGTGATTAAAAAGGAGAAGCTCAAAAAAGAGAGACTCATGAAACAAATCAAACGCGAAGTTTCTGTTATGAGTCTCGTTCGTCATCCTCACATCGTTGAACTCAAAGAGGTTATGGCGAACAAAGCCAAAGTGTTCATGGTTGTTGAATACGTCAAAGGCGGCGAGCTTTTCGCGAAGGTTGCGAAAGGGAAGATGAAGGAAGAGATTGCGAGGAAGTACTTTCAACAATTGATTAGTGCGGTTGATTTTTGTCATAGCCGCGGTGTTACGCATCGTGATTTGAAGCCGGAGAATCTGTTACTGGATGAGAATGAAGATCTTAAGGTTTCTGATTTTGGATTGTCGGCGTTGCCGGATCAACGACGATCCGACGGGATGCTTGTTACGCCGTGCGGGACTCCGGCTTACGTTGCGCCGGAGGTTTTAAAGAAGATTGGATATGATGGATCAAAGGCTGATATTTGGTCTTGTGGTGTGATTCTGTATGCTTTGCTTTGTGGATATTTGCCTTTTCAAGGTGAGAATGTCATGAGGATTTATAGTAAATCTTTTAAAGCGGATTATGCTTTACCGGAATGGATTTCACCGGGGGCCAAGAATTTGATTAGGAATTTGCTTGTTGTTGATCCTGAAAAGAGGTTTTCGATTGTTGATATAATGAAAGATCCTTGGTTTCAATTAGGGTTTATGAGACCTATTGCATTTTCAATGAAGGAATCTGCTGTGGATGATAACATTGATTTTAGTGGTGATGATGAAGCGAATAGTGATGGGAATAATGCCGAGGTTGTGGGCGGGATCGGTACGAATCATATGAGGAGGCCGTCTTATAATGCGTTTGAGATAATTTCGTCGTTGTCAAATGGATTTGATTTGAGGAACTTGTTTGAGACTAGGAAGAGATCACCGTCGATGTTTATATCGAAACTGTCTGCTTCGGCGGTGGTTGGGAAGCTGGAGAATGTGGCGAAGAAGCTGAATTTGAAGGTAACGGGGAAGAAGGATTTCATGGTGAGAATGCAGGGGACGACAGAGGGGAGGAAGGGGAAATTGGGGATGACAGTGGAGGTGTTTGAGGTTGCGCCGGAGGTGGCGGTGGTGGAGTTTTCTAAATCGTCTGGAGATACTCTTGAGTATGTGAAGTTTTGTGAAGATGAAGTTAGACCTTCTCTCAAAGACATTGTTTGGAGTTGGCAGGGTGATTCTCATTCATCATGCTAATTCTCAATCACATCAGTAATTTTATATTTTTTACATCAAAATCTAGAAATATCTCAGGCCTGTTAGATGTTTTCGTTAGTGTAATTCATTTAGATTGGCATTACAAGATTCATAAATGTTTGATATTTCATTTGAGGTTTGTAATTAGCTTCTAATAATACATCACTGATGTTGTTGGNACTT
TARGET=340,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3731
PRIMER_LEFT_PENALTY=0.039879
PRIMER_RIGHT_PENALTY=0.333229
PRIMER_LEFT_SEQUENCE=AAAACATGAAGCTCAACACCCT
PRIMER_RIGHT_SEQUENCE=TTCCTTGACCTAGCGTTTTACC
PRIMER_LEFT=193,22
PRIMER_RIGHT=432,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=59.667
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.3833
PRIMER_LEFT_1_PENALTY=0.050058
PRIMER_RIGHT_1_PENALTY=0.333229
PRIMER_LEFT_1_SEQUENCE=CTTTTACCAAAATACCCCTCCC
PRIMER_RIGHT_1_SEQUENCE=TTCCTTGACCTAGCGTTTTACC
PRIMER_LEFT_1=267,22
PRIMER_RIGHT_1=432,22
PRIMER_LEFT_1_TM=59.950
PRIMER_RIGHT_1_TM=59.667
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=166
PRIMER_PAIR_PENALTY_2=0.3833
PRIMER_LEFT_2_PENALTY=0.050058
PRIMER_RIGHT_2_PENALTY=0.333229
PRIMER_LEFT_2_SEQUENCE=CCTTTTACCAAAATACCCCTCC
PRIMER_RIGHT_2_SEQUENCE=TTCCTTGACCTAGCGTTTTACC
PRIMER_LEFT_2=266,22
PRIMER_RIGHT_2=432,22
PRIMER_LEFT_2_TM=59.950
PRIMER_RIGHT_2_TM=59.667
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.6665
PRIMER_LEFT_3_PENALTY=0.333282
PRIMER_RIGHT_3_PENALTY=0.333229
PRIMER_LEFT_3_SEQUENCE=CTAAAACCTCTTTCCACCCTCC
PRIMER_RIGHT_3_SEQUENCE=TTCCTTGACCTAGCGTTTTACC
PRIMER_LEFT_3=290,22
PRIMER_RIGHT_3=432,22
PRIMER_LEFT_3_TM=60.333
PRIMER_RIGHT_3_TM=59.667
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=0.6665
PRIMER_LEFT_4_PENALTY=0.333282
PRIMER_RIGHT_4_PENALTY=0.333229
PRIMER_LEFT_4_SEQUENCE=CCTAAAACCTCTTTCCACCCTC
PRIMER_RIGHT_4_SEQUENCE=TTCCTTGACCTAGCGTTTTACC
PRIMER_LEFT_4=289,22
PRIMER_RIGHT_4=432,22
PRIMER_LEFT_4_TM=60.333
PRIMER_RIGHT_4_TM=59.667
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_03265 Ps_contig_mira-and-tgicl-ass_franssen_29350
SEQUENCE=ATGGTCTGTTATGTTCTTATTTTTAATATTTAAGAAAGAAACTAATAATTAACATATTCATTCTTATATATGCCAATTTACATGCAAACCAAACTAAAAGCTGGGTGAGCTTTTTCAATTAGCACATAATATGTCAATAAAAATAAAATAAAATAAAAACAGAAATACACATAAATTTTTCTCTCTCTACATTATTTATTCTTAGATCCATCACTTCATCATCATAGTAAGAAAGAATTTCCGGTGCCTTTCCACCATACGGTATTGTACACCGCTTAATTAACATAGAAAGAGAGAAAAAACAACATTAGATTAGATCTAATCACTGTCGCTGCTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCATGGATCTTGTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTTTCTCCCCTTTGTGTTCATCATCACCATGGATCTTGTCCTTGATCTTCTCAACCAAACCTTCCTTGTGTTCACCTTTGTGCTTGTCATCGTGACTCTTCTCTCCTTTGTGTTCATCTTCTTTCTTGTGTCCTCCGATGTGAAGAGTCTCTCCGATCTTGTTGATGATTCCTGCCATTTTCTTTTCTGTGATTTTCTTTTGCTTCTATGTGACTTTCTGAGTTTATGTCAATTCCTCTCTTTCCTTCTCCTCTGCCTAATATCAATTCCAGCAGCCTTCAATTCTCTTTCTTCTGCAGAGAAGCAAGCCTCCTCGCTTCCTCAAGCTGTTTCTCCCTGGCTTTCCTTTTCGTCTTCTTACCTTTCGTGTAG
TARGET=140,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1237
PRIMER_LEFT_PENALTY=0.035021
PRIMER_RIGHT_PENALTY=1.088643
PRIMER_LEFT_SEQUENCE=AACCAAACTAAAAGCTGGGTGA
PRIMER_RIGHT_SEQUENCE=AATACCGTATGGTGGAAAGGC
PRIMER_LEFT=86,22
PRIMER_RIGHT=265,21
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=60.089
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=1.1781
PRIMER_LEFT_1_PENALTY=0.035021
PRIMER_RIGHT_1_PENALTY=1.143092
PRIMER_LEFT_1_SEQUENCE=AACCAAACTAAAAGCTGGGTGA
PRIMER_RIGHT_1_SEQUENCE=GGCACCGGAAATTCTTTCTTAC
PRIMER_LEFT_1=86,22
PRIMER_RIGHT_1=247,22
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=61.143
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=1.2106
PRIMER_LEFT_2_PENALTY=0.035021
PRIMER_RIGHT_2_PENALTY=1.175612
PRIMER_LEFT_2_SEQUENCE=AACCAAACTAAAAGCTGGGTGA
PRIMER_RIGHT_2_SEQUENCE=GCACCGGAAATTCTTTCTTACT
PRIMER_LEFT_2=86,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=60.035
PRIMER_RIGHT_2_TM=58.824
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=1.2842
PRIMER_LEFT_3_PENALTY=0.035021
PRIMER_RIGHT_3_PENALTY=1.249217
PRIMER_LEFT_3_SEQUENCE=AACCAAACTAAAAGCTGGGTGA
PRIMER_RIGHT_3_SEQUENCE=GAAGAAGGAACATGGTCATGGA
PRIMER_LEFT_3=86,22
PRIMER_RIGHT_3=378,22
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=61.249
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=1.2871
PRIMER_LEFT_4_PENALTY=0.035021
PRIMER_RIGHT_4_PENALTY=1.252044
PRIMER_LEFT_4_SEQUENCE=AACCAAACTAAAAGCTGGGTGA
PRIMER_RIGHT_4_SEQUENCE=AGGCACCGGAAATTCTTTCTTA
PRIMER_LEFT_4=86,22
PRIMER_RIGHT_4=248,22
PRIMER_LEFT_4_TM=60.035
PRIMER_RIGHT_4_TM=61.252
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_03266 Ps_contig_mira-and-tgicl-ass_franssen_29350
SEQUENCE=ATGGTCTGTTATGTTCTTATTTTTAATATTTAAGAAAGAAACTAATAATTAACATATTCATTCTTATATATGCCAATTTACATGCAAACCAAACTAAAAGCTGGGTGAGCTTTTTCAATTAGCACATAATATGTCAATAAAAATAAAATAAAATAAAAACAGAAATACACATAAATTTTTCTCTCTCTACATTATTTATTCTTAGATCCATCACTTCATCATCATAGTAAGAAAGAATTTCCGGTGCCTTTCCACCATACGGTATTGTACACCGCTTAATTAACATAGAAAGAGAGAAAAAACAACATTAGATTAGATCTAATCACTGTCGCTGCTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCATGGATCTTGTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTTTCTCCCCTTTGTGTTCATCATCACCATGGATCTTGTCCTTGATCTTCTCAACCAAACCTTCCTTGTGTTCACCTTTGTGCTTGTCATCGTGACTCTTCTCTCCTTTGTGTTCATCTTCTTTCTTGTGTCCTCCGATGTGAAGAGTCTCTCCGATCTTGTTGATGATTCCTGCCATTTTCTTTTCTGTGATTTTCTTTTGCTTCTATGTGACTTTCTGAGTTTATGTCAATTCCTCTCTTTCCTTCTCCTCTGCCTAATATCAATTCCAGCAGCCTTCAATTCTCTTTCTTCTGCAGAGAAGCAAGCCTCCTCGCTTCCTCAAGCTGTTTCTCCCTGGCTTTCCTTTTCGTCTTCTTACCTTTCGTGTAG
TARGET=331,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0291
PRIMER_LEFT_PENALTY=1.020829
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=CCATACGGTATTGTACACCGCT
PRIMER_RIGHT_SEQUENCE=TGAACATGGAAACAAAGGTGAG
PRIMER_LEFT=254,22
PRIMER_RIGHT=429,22
PRIMER_LEFT_TM=61.021
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=1.0330
PRIMER_LEFT_1_PENALTY=1.020829
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=CCATACGGTATTGTACACCGCT
PRIMER_RIGHT_1_SEQUENCE=AACACAAAGGGGAGAAAAGTCA
PRIMER_LEFT_1=254,22
PRIMER_RIGHT_1=526,22
PRIMER_LEFT_1_TM=61.021
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=1.0330
PRIMER_LEFT_2_PENALTY=1.020829
PRIMER_RIGHT_2_PENALTY=0.012153
PRIMER_LEFT_2_SEQUENCE=CCATACGGTATTGTACACCGCT
PRIMER_RIGHT_2_SEQUENCE=TGAACACAAAGGGGAGAAAAGT
PRIMER_LEFT_2=254,22
PRIMER_RIGHT_2=528,22
PRIMER_LEFT_2_TM=61.021
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=1.0402
PRIMER_LEFT_3_PENALTY=1.031989
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=CATACGGTATTGTACACCGCTT
PRIMER_RIGHT_3_SEQUENCE=TGAACATGGAAACAAAGGTGAG
PRIMER_LEFT_3=255,22
PRIMER_RIGHT_3=429,22
PRIMER_LEFT_3_TM=58.968
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=1.0441
PRIMER_LEFT_4_PENALTY=1.031989
PRIMER_RIGHT_4_PENALTY=0.012153
PRIMER_LEFT_4_SEQUENCE=CATACGGTATTGTACACCGCTT
PRIMER_RIGHT_4_SEQUENCE=AACACAAAGGGGAGAAAAGTCA
PRIMER_LEFT_4=255,22
PRIMER_RIGHT_4=526,22
PRIMER_LEFT_4_TM=58.968
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_03267 Ps_contig_mira-and-tgicl-ass_franssen_29350
SEQUENCE=ATGGTCTGTTATGTTCTTATTTTTAATATTTAAGAAAGAAACTAATAATTAACATATTCATTCTTATATATGCCAATTTACATGCAAACCAAACTAAAAGCTGGGTGAGCTTTTTCAATTAGCACATAATATGTCAATAAAAATAAAATAAAATAAAAACAGAAATACACATAAATTTTTCTCTCTCTACATTATTTATTCTTAGATCCATCACTTCATCATCATAGTAAGAAAGAATTTCCGGTGCCTTTCCACCATACGGTATTGTACACCGCTTAATTAACATAGAAAGAGAGAAAAAACAACATTAGATTAGATCTAATCACTGTCGCTGCTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCATGGATCTTGTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTTTCTCCCCTTTGTGTTCATCATCACCATGGATCTTGTCCTTGATCTTCTCAACCAAACCTTCCTTGTGTTCACCTTTGTGCTTGTCATCGTGACTCTTCTCTCCTTTGTGTTCATCTTCTTTCTTGTGTCCTCCGATGTGAAGAGTCTCTCCGATCTTGTTGATGATTCCTGCCATTTTCTTTTCTGTGATTTTCTTTTGCTTCTATGTGACTTTCTGAGTTTATGTCAATTCCTCTCTTTCCTTCTCCTCTGCCTAATATCAATTCCAGCAGCCTTCAATTCTCTTTCTTCTGCAGAGAAGCAAGCCTCCTCGCTTCCTCAAGCTGTTTCTCCCTGGCTTTCCTTTTCGTCTTCTTACCTTTCGTGTAG
TARGET=373,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0527
PRIMER_LEFT_PENALTY=0.044457
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=GATCTAATCACTGTCGCTGCTG
PRIMER_RIGHT_SEQUENCE=TGAACATGGAAACAAAGGTGAG
PRIMER_LEFT=315,22
PRIMER_RIGHT=429,22
PRIMER_LEFT_TM=60.044
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=0.0566
PRIMER_LEFT_1_PENALTY=0.044457
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=GATCTAATCACTGTCGCTGCTG
PRIMER_RIGHT_1_SEQUENCE=TGAACACAAAGGGGAGAAAAGT
PRIMER_LEFT_1=315,22
PRIMER_RIGHT_1=528,22
PRIMER_LEFT_1_TM=60.044
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=0.0566
PRIMER_LEFT_2_PENALTY=0.044457
PRIMER_RIGHT_2_PENALTY=0.012153
PRIMER_LEFT_2_SEQUENCE=GATCTAATCACTGTCGCTGCTG
PRIMER_RIGHT_2_SEQUENCE=AACACAAAGGGGAGAAAAGTCA
PRIMER_LEFT_2=315,22
PRIMER_RIGHT_2=526,22
PRIMER_LEFT_2_TM=60.044
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.0566
PRIMER_LEFT_3_PENALTY=0.044457
PRIMER_RIGHT_3_PENALTY=0.012153
PRIMER_LEFT_3_SEQUENCE=GATCTAATCACTGTCGCTGCTG
PRIMER_RIGHT_3_SEQUENCE=ACAAGGAAGGTTTGGTTGAGAA
PRIMER_LEFT_3=315,22
PRIMER_RIGHT_3=577,22
PRIMER_LEFT_3_TM=60.044
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=0.0680
PRIMER_LEFT_4_PENALTY=0.044457
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=GATCTAATCACTGTCGCTGCTG
PRIMER_RIGHT_4_SEQUENCE=GGTTTGGTTGAGAAGATCAAGG
PRIMER_LEFT_4=315,22
PRIMER_RIGHT_4=569,22
PRIMER_LEFT_4_TM=60.044
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_03268 Ps_contig_mira-and-tgicl-ass_franssen_29350
SEQUENCE=ATGGTCTGTTATGTTCTTATTTTTAATATTTAAGAAAGAAACTAATAATTAACATATTCATTCTTATATATGCCAATTTACATGCAAACCAAACTAAAAGCTGGGTGAGCTTTTTCAATTAGCACATAATATGTCAATAAAAATAAAATAAAATAAAAACAGAAATACACATAAATTTTTCTCTCTCTACATTATTTATTCTTAGATCCATCACTTCATCATCATAGTAAGAAAGAATTTCCGGTGCCTTTCCACCATACGGTATTGTACACCGCTTAATTAACATAGAAAGAGAGAAAAAACAACATTAGATTAGATCTAATCACTGTCGCTGCTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCATGGATCTTGTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTTTCTCCCCTTTGTGTTCATCATCACCATGGATCTTGTCCTTGATCTTCTCAACCAAACCTTCCTTGTGTTCACCTTTGTGCTTGTCATCGTGACTCTTCTCTCCTTTGTGTTCATCTTCTTTCTTGTGTCCTCCGATGTGAAGAGTCTCTCCGATCTTGTTGATGATTCCTGCCATTTTCTTTTCTGTGATTTTCTTTTGCTTCTATGTGACTTTCTGAGTTTATGTCAATTCCTCTCTTTCCTTCTCCTCTGCCTAATATCAATTCCAGCAGCCTTCAATTCTCTTTCTTCTGCAGAGAAGCAAGCCTCCTCGCTTCCTCAAGCTGTTTCTCCCTGGCTTTCCTTTTCGTCTTCTTACCTTTCGTGTAG
TARGET=388,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0527
PRIMER_LEFT_PENALTY=0.044457
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=GATCTAATCACTGTCGCTGCTG
PRIMER_RIGHT_SEQUENCE=TGAACATGGAAACAAAGGTGAG
PRIMER_LEFT=315,22
PRIMER_RIGHT=429,22
PRIMER_LEFT_TM=60.044
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=0.0566
PRIMER_LEFT_1_PENALTY=0.044457
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=GATCTAATCACTGTCGCTGCTG
PRIMER_RIGHT_1_SEQUENCE=TGAACACAAAGGGGAGAAAAGT
PRIMER_LEFT_1=315,22
PRIMER_RIGHT_1=528,22
PRIMER_LEFT_1_TM=60.044
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=0.0566
PRIMER_LEFT_2_PENALTY=0.044457
PRIMER_RIGHT_2_PENALTY=0.012153
PRIMER_LEFT_2_SEQUENCE=GATCTAATCACTGTCGCTGCTG
PRIMER_RIGHT_2_SEQUENCE=AACACAAAGGGGAGAAAAGTCA
PRIMER_LEFT_2=315,22
PRIMER_RIGHT_2=526,22
PRIMER_LEFT_2_TM=60.044
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.0566
PRIMER_LEFT_3_PENALTY=0.044457
PRIMER_RIGHT_3_PENALTY=0.012153
PRIMER_LEFT_3_SEQUENCE=GATCTAATCACTGTCGCTGCTG
PRIMER_RIGHT_3_SEQUENCE=ACAAGGAAGGTTTGGTTGAGAA
PRIMER_LEFT_3=315,22
PRIMER_RIGHT_3=577,22
PRIMER_LEFT_3_TM=60.044
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=0.0680
PRIMER_LEFT_4_PENALTY=0.044457
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=GATCTAATCACTGTCGCTGCTG
PRIMER_RIGHT_4_SEQUENCE=GGTTTGGTTGAGAAGATCAAGG
PRIMER_LEFT_4=315,22
PRIMER_RIGHT_4=569,22
PRIMER_LEFT_4_TM=60.044
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_03269 Ps_contig_mira-and-tgicl-ass_franssen_29351
SEQUENCE=TCTGTTTATTATATTCTTATTTTTTAATTATTTAAGAAAGAAACTAATAATTAACATATTCATTCTTATATATGCCAATTTACATGCAAACCTGGGTGAGTCTTTTTCAATTAGCACATAATATGTCAATAAAATAAAATAAAATAAAAATAGAAATACACATAAATTTCTCTCTCTCTCCATCTCTCTACATTATTTATTCTTAGATCCATCACTTCATCATCATAGTAAGAAAGAATTTCCGGTGCCTCTCCACCATACGGTATTGTACACCGCTTAATTAACATAGAAAGAGAAGAAAAAAACAACATTAGATTAGATCTAATCGCTGTCGCTGCTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCATGGATCTTGTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTTTCTCCCCTTTGTGTTCATCATCACCATGGATCTTGTCCTTGATCTTCCTACCAAACCCTAAACCTTTCCTTTGTTGTTCCTACCTTTGGTCGTCTTGTCATCGT
TARGET=128,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1552
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=1.143092
PRIMER_LEFT_SEQUENCE=CAAACCTGGGTGAGTCTTTTTC
PRIMER_RIGHT_SEQUENCE=GGCACCGGAAATTCTTTCTTAC
PRIMER_LEFT=86,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=61.143
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=1.1552
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=1.143092
PRIMER_LEFT_1_SEQUENCE=AAACCTGGGTGAGTCTTTTTCA
PRIMER_RIGHT_1_SEQUENCE=GGCACCGGAAATTCTTTCTTAC
PRIMER_LEFT_1=87,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=61.143
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=1.1552
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=1.143092
PRIMER_LEFT_2_SEQUENCE=AACCTGGGTGAGTCTTTTTCAA
PRIMER_RIGHT_2_SEQUENCE=GGCACCGGAAATTCTTTCTTAC
PRIMER_LEFT_2=88,22
PRIMER_RIGHT_2=248,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=61.143
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=1.1878
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=1.175612
PRIMER_LEFT_3_SEQUENCE=CAAACCTGGGTGAGTCTTTTTC
PRIMER_RIGHT_3_SEQUENCE=GCACCGGAAATTCTTTCTTACT
PRIMER_LEFT_3=86,22
PRIMER_RIGHT_3=247,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=58.824
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=1.1878
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=1.175612
PRIMER_LEFT_4_SEQUENCE=AAACCTGGGTGAGTCTTTTTCA
PRIMER_RIGHT_4_SEQUENCE=GCACCGGAAATTCTTTCTTACT
PRIMER_LEFT_4=87,22
PRIMER_RIGHT_4=247,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=58.824
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_03270 Ps_contig_mira-and-tgicl-ass_franssen_29351
SEQUENCE=TCTGTTTATTATATTCTTATTTTTTAATTATTTAAGAAAGAAACTAATAATTAACATATTCATTCTTATATATGCCAATTTACATGCAAACCTGGGTGAGTCTTTTTCAATTAGCACATAATATGTCAATAAAATAAAATAAAATAAAAATAGAAATACACATAAATTTCTCTCTCTCTCCATCTCTCTACATTATTTATTCTTAGATCCATCACTTCATCATCATAGTAAGAAAGAATTTCCGGTGCCTCTCCACCATACGGTATTGTACACCGCTTAATTAACATAGAAAGAGAAGAAAAAAACAACATTAGATTAGATCTAATCGCTGTCGCTGCTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCATGGATCTTGTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTTTCTCCCCTTTGTGTTCATCATCACCATGGATCTTGTCCTTGATCTTCCTACCAAACCCTAAACCTTTCCTTTGTTGTTCCTACCTTTGGTCGTCTTGTCATCGT
TARGET=169,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1552
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=1.143092
PRIMER_LEFT_SEQUENCE=CAAACCTGGGTGAGTCTTTTTC
PRIMER_RIGHT_SEQUENCE=GGCACCGGAAATTCTTTCTTAC
PRIMER_LEFT=86,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=61.143
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=1.1552
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=1.143092
PRIMER_LEFT_1_SEQUENCE=AAACCTGGGTGAGTCTTTTTCA
PRIMER_RIGHT_1_SEQUENCE=GGCACCGGAAATTCTTTCTTAC
PRIMER_LEFT_1=87,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=61.143
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=1.1552
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=1.143092
PRIMER_LEFT_2_SEQUENCE=AACCTGGGTGAGTCTTTTTCAA
PRIMER_RIGHT_2_SEQUENCE=GGCACCGGAAATTCTTTCTTAC
PRIMER_LEFT_2=88,22
PRIMER_RIGHT_2=248,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=61.143
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=1.1878
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=1.175612
PRIMER_LEFT_3_SEQUENCE=CAAACCTGGGTGAGTCTTTTTC
PRIMER_RIGHT_3_SEQUENCE=GCACCGGAAATTCTTTCTTACT
PRIMER_LEFT_3=86,22
PRIMER_RIGHT_3=247,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=58.824
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=1.1878
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=1.175612
PRIMER_LEFT_4_SEQUENCE=AAACCTGGGTGAGTCTTTTTCA
PRIMER_RIGHT_4_SEQUENCE=GCACCGGAAATTCTTTCTTACT
PRIMER_LEFT_4=87,22
PRIMER_RIGHT_4=247,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=58.824
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_03271 Ps_contig_mira-and-tgicl-ass_franssen_29351
SEQUENCE=TCTGTTTATTATATTCTTATTTTTTAATTATTTAAGAAAGAAACTAATAATTAACATATTCATTCTTATATATGCCAATTTACATGCAAACCTGGGTGAGTCTTTTTCAATTAGCACATAATATGTCAATAAAATAAAATAAAATAAAAATAGAAATACACATAAATTTCTCTCTCTCTCCATCTCTCTACATTATTTATTCTTAGATCCATCACTTCATCATCATAGTAAGAAAGAATTTCCGGTGCCTCTCCACCATACGGTATTGTACACCGCTTAATTAACATAGAAAGAGAAGAAAAAAACAACATTAGATTAGATCTAATCGCTGTCGCTGCTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCATGGATCTTGTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTTTCTCCCCTTTGTGTTCATCATCACCATGGATCTTGTCCTTGATCTTCCTACCAAACCCTAAACCTTTCCTTTGTTGTTCCTACCTTTGGTCGTCTTGTCATCGT
TARGET=334,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0291
PRIMER_LEFT_PENALTY=1.020829
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=CCATACGGTATTGTACACCGCT
PRIMER_RIGHT_SEQUENCE=TGAACATGGAAACAAAGGTGAG
PRIMER_LEFT=255,22
PRIMER_RIGHT=432,22
PRIMER_LEFT_TM=61.021
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=1.0330
PRIMER_LEFT_1_PENALTY=1.020829
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=CCATACGGTATTGTACACCGCT
PRIMER_RIGHT_1_SEQUENCE=AACACAAAGGGGAGAAAAGTCA
PRIMER_LEFT_1=255,22
PRIMER_RIGHT_1=529,22
PRIMER_LEFT_1_TM=61.021
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=1.0330
PRIMER_LEFT_2_PENALTY=1.020829
PRIMER_RIGHT_2_PENALTY=0.012153
PRIMER_LEFT_2_SEQUENCE=CCATACGGTATTGTACACCGCT
PRIMER_RIGHT_2_SEQUENCE=TGAACACAAAGGGGAGAAAAGT
PRIMER_LEFT_2=255,22
PRIMER_RIGHT_2=531,22
PRIMER_LEFT_2_TM=61.021
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=1.0402
PRIMER_LEFT_3_PENALTY=1.031989
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=CATACGGTATTGTACACCGCTT
PRIMER_RIGHT_3_SEQUENCE=TGAACATGGAAACAAAGGTGAG
PRIMER_LEFT_3=256,22
PRIMER_RIGHT_3=432,22
PRIMER_LEFT_3_TM=58.968
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=1.0441
PRIMER_LEFT_4_PENALTY=1.031989
PRIMER_RIGHT_4_PENALTY=0.012153
PRIMER_LEFT_4_SEQUENCE=CATACGGTATTGTACACCGCTT
PRIMER_RIGHT_4_SEQUENCE=AACACAAAGGGGAGAAAAGTCA
PRIMER_LEFT_4=256,22
PRIMER_RIGHT_4=529,22
PRIMER_LEFT_4_TM=58.968
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_03272 Ps_contig_mira-and-tgicl-ass_franssen_29351
SEQUENCE=TCTGTTTATTATATTCTTATTTTTTAATTATTTAAGAAAGAAACTAATAATTAACATATTCATTCTTATATATGCCAATTTACATGCAAACCTGGGTGAGTCTTTTTCAATTAGCACATAATATGTCAATAAAATAAAATAAAATAAAAATAGAAATACACATAAATTTCTCTCTCTCTCCATCTCTCTACATTATTTATTCTTAGATCCATCACTTCATCATCATAGTAAGAAAGAATTTCCGGTGCCTCTCCACCATACGGTATTGTACACCGCTTAATTAACATAGAAAGAGAAGAAAAAAACAACATTAGATTAGATCTAATCGCTGTCGCTGCTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCATGGATCTTGTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTTTCTCCCCTTTGTGTTCATCATCACCATGGATCTTGTCCTTGATCTTCCTACCAAACCCTAAACCTTTCCTTTGTTGTTCCTACCTTTGGTCGTCTTGTCATCGT
TARGET=376,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0291
PRIMER_LEFT_PENALTY=1.020829
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=CCATACGGTATTGTACACCGCT
PRIMER_RIGHT_SEQUENCE=TGAACATGGAAACAAAGGTGAG
PRIMER_LEFT=255,22
PRIMER_RIGHT=432,22
PRIMER_LEFT_TM=61.021
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=1.0330
PRIMER_LEFT_1_PENALTY=1.020829
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=CCATACGGTATTGTACACCGCT
PRIMER_RIGHT_1_SEQUENCE=AACACAAAGGGGAGAAAAGTCA
PRIMER_LEFT_1=255,22
PRIMER_RIGHT_1=529,22
PRIMER_LEFT_1_TM=61.021
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=1.0330
PRIMER_LEFT_2_PENALTY=1.020829
PRIMER_RIGHT_2_PENALTY=0.012153
PRIMER_LEFT_2_SEQUENCE=CCATACGGTATTGTACACCGCT
PRIMER_RIGHT_2_SEQUENCE=TGAACACAAAGGGGAGAAAAGT
PRIMER_LEFT_2=255,22
PRIMER_RIGHT_2=531,22
PRIMER_LEFT_2_TM=61.021
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=1.0402
PRIMER_LEFT_3_PENALTY=1.031989
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=CATACGGTATTGTACACCGCTT
PRIMER_RIGHT_3_SEQUENCE=TGAACATGGAAACAAAGGTGAG
PRIMER_LEFT_3=256,22
PRIMER_RIGHT_3=432,22
PRIMER_LEFT_3_TM=58.968
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=1.0441
PRIMER_LEFT_4_PENALTY=1.031989
PRIMER_RIGHT_4_PENALTY=0.012153
PRIMER_LEFT_4_SEQUENCE=CATACGGTATTGTACACCGCTT
PRIMER_RIGHT_4_SEQUENCE=AACACAAAGGGGAGAAAAGTCA
PRIMER_LEFT_4=256,22
PRIMER_RIGHT_4=529,22
PRIMER_LEFT_4_TM=58.968
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_03273 Ps_contig_mira-and-tgicl-ass_franssen_29351
SEQUENCE=TCTGTTTATTATATTCTTATTTTTTAATTATTTAAGAAAGAAACTAATAATTAACATATTCATTCTTATATATGCCAATTTACATGCAAACCTGGGTGAGTCTTTTTCAATTAGCACATAATATGTCAATAAAATAAAATAAAATAAAAATAGAAATACACATAAATTTCTCTCTCTCTCCATCTCTCTACATTATTTATTCTTAGATCCATCACTTCATCATCATAGTAAGAAAGAATTTCCGGTGCCTCTCCACCATACGGTATTGTACACCGCTTAATTAACATAGAAAGAGAAGAAAAAAACAACATTAGATTAGATCTAATCGCTGTCGCTGCTGCTGCTACTATCATGATCATGTCCATGACCATGTTCCTTCTTCTTCTTATCCTTCTTCTTCTTGTCCTTCTTCTCACCTTTGTTTCCATGTTCATCACTTCCACCATGGATCTTGTCCTTAATCTTCTCAACTACACCTTCTTTGTGTTCACCTTTGTGCTTATCATCGTGACTTTTCTCCCCTTTGTGTTCATCATCACCATGGATCTTGTCCTTGATCTTCCTACCAAACCCTAAACCTTTCCTTTGTTGTTCCTACCTTTGGTCGTCTTGTCATCGT
TARGET=391,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2454
PRIMER_LEFT_PENALTY=0.233224
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=GTCCATGACCATGTTCCTTCTT
PRIMER_RIGHT_SEQUENCE=TGAACACAAAGGGGAGAAAAGT
PRIMER_LEFT=359,22
PRIMER_RIGHT=531,22
PRIMER_LEFT_TM=60.233
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.3324
PRIMER_LEFT_1_PENALTY=0.233224
PRIMER_RIGHT_1_PENALTY=0.099187
PRIMER_LEFT_1_SEQUENCE=GTCCATGACCATGTTCCTTCTT
PRIMER_RIGHT_1_SEQUENCE=CCAAAGGTAGGAACAACAAAGG
PRIMER_LEFT_1=359,22
PRIMER_RIGHT_1=603,22
PRIMER_LEFT_1_TM=60.233
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.3641
PRIMER_LEFT_2_PENALTY=0.233224
PRIMER_RIGHT_2_PENALTY=0.130883
PRIMER_LEFT_2_SEQUENCE=GTCCATGACCATGTTCCTTCTT
PRIMER_RIGHT_2_SEQUENCE=CGATGATAAGCACAAAGGTGAA
PRIMER_LEFT_2=359,22
PRIMER_RIGHT_2=507,22
PRIMER_LEFT_2_TM=60.233
PRIMER_RIGHT_2_TM=60.131
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.4606
PRIMER_LEFT_3_PENALTY=0.361394
PRIMER_RIGHT_3_PENALTY=0.099187
PRIMER_LEFT_3_SEQUENCE=CCATGACCATGTTCCTTCTTCT
PRIMER_RIGHT_3_SEQUENCE=CCAAAGGTAGGAACAACAAAGG
PRIMER_LEFT_3=361,22
PRIMER_RIGHT_3=603,22
PRIMER_LEFT_3_TM=60.361
PRIMER_RIGHT_3_TM=59.901
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.4718
PRIMER_LEFT_4_PENALTY=0.233224
PRIMER_RIGHT_4_PENALTY=0.238588
PRIMER_LEFT_4_SEQUENCE=GTCCATGACCATGTTCCTTCTT
PRIMER_RIGHT_4_SEQUENCE=AAAGGAAAGGTTTAGGGTTTGG
PRIMER_LEFT_4=359,22
PRIMER_RIGHT_4=586,22
PRIMER_LEFT_4_TM=60.233
PRIMER_RIGHT_4_TM=59.761
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_03274 Ps_contig_mira-and-tgicl-ass_franssen_29356
SEQUENCE=CAACATTTATTTTACGTTTGTTGTTAGAAGAATACATGAGGAGAGAATCCAAGTCCCCCAAAAGAATCAGTGTGTATGTGTTATAAAAGTTAGAAAAATGCGAACATGCTGACAAACGGAGCAATTTGAGACTAAACAACTTTTAAGCATCAATCAAAAATAAAACAAAAAGACAAATTTGAGACTAATCAACTGTCAAGTATCAATAAAAAATTAAACAATAGTTCGACGCAACTTTAAAAGTTTGTCACCAGTTAAATAGAATTTATTGAATTAATTTCTTTCCCTAGTTGTAAGCATCTTAGAGAGAGTACACATGCATACTCCAAAAAATGCCAAAGGCAGTTACATGCTCAATGAGGATCCAGAGTTCTTTCTGCATTCTGATAGCATATCCAAGTAAAACTGACACTTGCTAATCTCACTTCCGTAATTGTTCAGGCAATCCTGGAAAGCCTTTGAATGAATATTGCATGCATCACCACCAAAAGAGCTGGCAGTTGGTGCAGGGGCAGCAGCAGCAGCAGCCTCACTAGCTACAGTTTCATGTTGAATAGTGCGAGGACCCATAACGGCGTCCACAGCCCTATGTGCCACTGCACTTCCAGTGCCAAAAGCCATACCTTGAGCTATGGTTGAACCAATACCACTAAGCATAGATCCACCGCCACTCTGAACATTAGCCGGAGGAGGAGCATGGTTGACTGGAGCGGGGCGAGCTGGTGCAGCTCGGGGAGCTGGACGAGCAGATCTTCCGCCGGAGCTACGACGAGCCATTGATAAAGTTTTAGGGCAAAGAAGCAAAAGAGAGCAAAGGGGATTTTAGGTGGCAGAATTGATCTTGTCTTATGTATGTGCCCAGTCT
TARGET=512,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3504
PRIMER_LEFT_PENALTY=0.219551
PRIMER_RIGHT_PENALTY=0.130883
PRIMER_LEFT_SEQUENCE=GCTCAATGAGGATCCAGAGTTC
PRIMER_RIGHT_SEQUENCE=CCTCGCACTATTCAACATGAAA
PRIMER_LEFT=351,22
PRIMER_RIGHT=563,22
PRIMER_LEFT_TM=60.220
PRIMER_RIGHT_TM=60.131
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.3571
PRIMER_LEFT_1_PENALTY=0.219551
PRIMER_RIGHT_1_PENALTY=0.137550
PRIMER_LEFT_1_SEQUENCE=GCTCAATGAGGATCCAGAGTTC
PRIMER_RIGHT_1_SEQUENCE=GTTATGGGTCCTCGCACTATTC
PRIMER_LEFT_1=351,22
PRIMER_RIGHT_1=572,22
PRIMER_LEFT_1_TM=60.220
PRIMER_RIGHT_1_TM=59.862
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.3593
PRIMER_LEFT_2_PENALTY=0.219551
PRIMER_RIGHT_2_PENALTY=0.139753
PRIMER_LEFT_2_SEQUENCE=GCTCAATGAGGATCCAGAGTTC
PRIMER_RIGHT_2_SEQUENCE=GTCCTCGCACTATTCAACATGA
PRIMER_LEFT_2=351,22
PRIMER_RIGHT_2=565,22
PRIMER_LEFT_2_TM=60.220
PRIMER_RIGHT_2_TM=60.140
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=0.4944
PRIMER_LEFT_3_PENALTY=0.363508
PRIMER_RIGHT_3_PENALTY=0.130883
PRIMER_LEFT_3_SEQUENCE=CTTCCGTAATTGTTCAGGCAAT
PRIMER_RIGHT_3_SEQUENCE=CCTCGCACTATTCAACATGAAA
PRIMER_LEFT_3=424,22
PRIMER_RIGHT_3=563,22
PRIMER_LEFT_3_TM=60.364
PRIMER_RIGHT_3_TM=60.131
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=140
PRIMER_PAIR_PENALTY_4=0.5011
PRIMER_LEFT_4_PENALTY=0.363508
PRIMER_RIGHT_4_PENALTY=0.137550
PRIMER_LEFT_4_SEQUENCE=CTTCCGTAATTGTTCAGGCAAT
PRIMER_RIGHT_4_SEQUENCE=GTTATGGGTCCTCGCACTATTC
PRIMER_LEFT_4=424,22
PRIMER_RIGHT_4=572,22
PRIMER_LEFT_4_TM=60.364
PRIMER_RIGHT_4_TM=59.862
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_03275 Ps_contig_mira-and-tgicl-ass_franssen_29361
SEQUENCE=CTAACGCAGAGTANTCGGGGATAGTATTCAGTAATTTCATTCTTTGTTCTTTTCTTAAGAAAAGAAAAAGAGCTTTCTATATCCATCTGAGTAGTCATGTTGGGTGTATTCAGCAGCTCCGTCGTGTCCCCACCGGACGAGCTCGTTGCTGCCGGCAGCCGAACACCGTCGCCGAAAACGACATCGGCGGCTCTGCTTAAGCGTTTCACTGAGAGCAACGCGTCGACAGTGTCGGTAGAAGTCGGAGATCAAGTCCGGTTTGCCTATACCCACCAGAACGAGTCGTCGTTGCAACCGAGAATGTTTGGTGTAAAAGATGAAATATTCTGCATGTTTGAGGGAGCTCTTGATAATCTGGGTAGCTTAAGGCAACAATATGGACTGGCCAAGTCTGCAAATGAAGTAGTTTTGGTGATTGAGGCTTACAAAGCTTTACGCGACAGAGCACCTTATCCTCCTAACCATGTTGTTGGTCATCTCAGTGGCGACTTTGCTTTCATACTTTTCGACAAATCTACTTCCACCCTATTTGTTGCATCTGATCAATTTGGTAAGGTGCCACTGTTTTGGGGCATAACTGCTGATGGATATGTAGTGTTTGCTGATGATGCTGAATTGCTTAAAGGTGCTTGTGGCAAGTCACTTGCTTCTTTCCCTCAAGGATGTTTCTACTCGACGGCCGTGGGAGGATTGATGTGCTATGAGAATCCTAAAAACAAGATTACTGCTGTGCCTGCCAAAGAAGAAGAAATCTGGGGTGCAACCTTCAAAGTGGAAGGTGCAACAGTTGTAGCAGCCAGAGAGTGATTTGAGTTGAGAATGAGATCACAATGAGGGGGCAATGTAATTAAATGGCTTGACGAGTGAAGCAAAGTTGTAGAAGTGTAACCTTGTTCATTTCATGTATAATTTAATGTTTCAATCAATGTATAGTTTGGCCTTTTCTATCAATAATAATAATAATGGTTGTGGTTCTATACTGTCCTTACTCTCTTCAATTTCACAAACAAACACACATATGCACTGCTTGGTGCCTTTACCATCAATCATCAACTAAACTTATTTAAGG
TARGET=950,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2128
PRIMER_LEFT_PENALTY=0.041981
PRIMER_RIGHT_PENALTY=0.170825
PRIMER_LEFT_SEQUENCE=TTGACGAGTGAAGCAAAGTTGT
PRIMER_RIGHT_SEQUENCE=TGATGATTGATGGTAAAGGCAC
PRIMER_LEFT=856,22
PRIMER_RIGHT=1051,22
PRIMER_LEFT_TM=59.958
PRIMER_RIGHT_TM=59.829
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.3248
PRIMER_LEFT_1_PENALTY=0.154023
PRIMER_RIGHT_1_PENALTY=0.170825
PRIMER_LEFT_1_SEQUENCE=TAGCAGCCAGAGAGTGATTTGA
PRIMER_RIGHT_1_SEQUENCE=TGATGATTGATGGTAAAGGCAC
PRIMER_LEFT_1=790,22
PRIMER_RIGHT_1=1051,22
PRIMER_LEFT_1_TM=60.154
PRIMER_RIGHT_1_TM=59.829
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.4220
PRIMER_LEFT_2_PENALTY=0.251137
PRIMER_RIGHT_2_PENALTY=0.170825
PRIMER_LEFT_2_SEQUENCE=CAGTTGTAGCAGCCAGAGAGTG
PRIMER_RIGHT_2_SEQUENCE=TGATGATTGATGGTAAAGGCAC
PRIMER_LEFT_2=784,22
PRIMER_RIGHT_2=1051,22
PRIMER_LEFT_2_TM=60.251
PRIMER_RIGHT_2_TM=59.829
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.4984
PRIMER_LEFT_3_PENALTY=0.327533
PRIMER_RIGHT_3_PENALTY=0.170825
PRIMER_LEFT_3_SEQUENCE=AGAATGAGATCACAATGAGGGG
PRIMER_RIGHT_3_SEQUENCE=TGATGATTGATGGTAAAGGCAC
PRIMER_LEFT_3=816,22
PRIMER_RIGHT_3=1051,22
PRIMER_LEFT_3_TM=60.328
PRIMER_RIGHT_3_TM=59.829
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.5465
PRIMER_LEFT_4_PENALTY=0.041981
PRIMER_RIGHT_4_PENALTY=0.504551
PRIMER_LEFT_4_SEQUENCE=TTGACGAGTGAAGCAAAGTTGT
PRIMER_RIGHT_4_SEQUENCE=ACCAAGCAGTGCATATGTGTGT
PRIMER_LEFT_4=856,22
PRIMER_RIGHT_4=1031,22
PRIMER_LEFT_4_TM=59.958
PRIMER_RIGHT_4_TM=60.505
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=176
=
PRIMER_SEQUENCE_ID=SSR_03276 Ps_contig_mira-and-tgicl-ass_franssen_29362
SEQUENCE=AGGCAGAAGCGCATTTACGTGAATTTGTGCTTACAACAAATCATATAGAGGTGCATTTCGGGAGTTAGGGTTTTCCTCAGCCGCCGTAGCCGCCACAACCACCAGCAGCACCACCACCACCACCAACGAAGCAATGGTTCTCAAGACTGAACTCTGCCGATTCAGTGGCGCAAAGATCTACCCAGGAAGAGGCATCAGATTTATTCGTAGTGACTCACAGGTTTTTCTATTTGTGAACTCGAAATGTAAGAGGTATTTCCACAACCGTTTGAAGCCTTCAAAGCTCACATGGACTGCCATGTACAGAAAGCAACACAAGAAGGACATTGCCCAAGAAGCTGTGAAGAAGAGACGCCGTGCTACCAAGAAGCCATACTCTAGGTCCATTGTAGGTGCCACTTTGGAAGTCATTCAGAAGAAAAGAGCTGAGAAGCCTGAAGTTCGAGATGCCGCCAGGGAAGCTGCTCTGCGTGAAATCAAAGAGAGGATTAAGAAAACAAAGGATGAGAAGAAAGCCAAGAAAGCAGAAGTAGCATCTAAGGCACAAAAATCTGGCAAAGGCAATGTTCAGAAAGGTGGTTTGCCCAAGGGTCCTAAATTGGGCGGTGGCGGCGGTAAACGCTGATCAGTTCCAATTTTGGCTACAAATTAAATACTTTTTGGTTGAATCTTTTGTATCAATATTTTATTTGCTTGTTTAGCCTTTGTTGCCTAATTACCAGACTTATTTTGGAGCTTCAGACAGTAGTTGGTTACTGAATGCATTTTGCTAGTGGTTCTTTATTTTGTTGCACTAACGATTTTATAACGCATGTTGTTTTAATTGGGAGTTTGACTTTGAATATCAAATGCTCAAACATTAATTATATCGGAAGAGACTTAATTGCATCTTCTATGGACC
TARGET=109,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1088
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=0.095396
PRIMER_LEFT_SEQUENCE=TATAGAGGTGCATTTCGGGAGT
PRIMER_RIGHT_SEQUENCE=GCCTCTTCCTGGGTAGATCTTT
PRIMER_LEFT=43,22
PRIMER_RIGHT=192,22
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=150
PRIMER_PAIR_PENALTY_1=0.3263
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=0.312895
PRIMER_LEFT_1_SEQUENCE=TATAGAGGTGCATTTCGGGAGT
PRIMER_RIGHT_1_SEQUENCE=TTGCTTTCTGTACATGGCAGTC
PRIMER_LEFT_1=43,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=60.313
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.5865
PRIMER_LEFT_2_PENALTY=0.013401
PRIMER_RIGHT_2_PENALTY=0.573098
PRIMER_LEFT_2_SEQUENCE=TATAGAGGTGCATTTCGGGAGT
PRIMER_RIGHT_2_SEQUENCE=TAAATCTGATGCCTCTTCCTGG
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=202,22
PRIMER_LEFT_2_TM=59.987
PRIMER_RIGHT_2_TM=60.573
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=0.6106
PRIMER_LEFT_3_PENALTY=0.515196
PRIMER_RIGHT_3_PENALTY=0.095396
PRIMER_LEFT_3_SEQUENCE=ATATAGAGGTGCATTTCGGGAG
PRIMER_RIGHT_3_SEQUENCE=GCCTCTTCCTGGGTAGATCTTT
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=192,22
PRIMER_LEFT_3_TM=59.485
PRIMER_RIGHT_3_TM=60.095
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.6362
PRIMER_LEFT_4_PENALTY=0.013401
PRIMER_RIGHT_4_PENALTY=0.622772
PRIMER_LEFT_4_SEQUENCE=TATAGAGGTGCATTTCGGGAGT
PRIMER_RIGHT_4_SEQUENCE=TCAAACGGTTGTGGAAATACCT
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=271,22
PRIMER_LEFT_4_TM=59.987
PRIMER_RIGHT_4_TM=60.623
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_03277 Ps_contig_mira-and-tgicl-ass_franssen_29364
SEQUENCE=AACGTTTTTTTTTAAATTTTAACTTTGTAAAATAGCAGCATGTATGTCAATAGACAAACTAATAACATTTTCTTGATTAGTTTGGTAGANATAATTTTTTCTTAAACAACATCCACCATTTAATCCTTACAAGTACATTTACTGTGACATAAGAGGTGAAAAAGGGGCTGAAGAAAAGCGCATTGCTCAAGTAACAACAAAGGGAAAACTGCAATTAGCTACAATCCTGGTCCCCTAAAAGAACCTCCTCACATACTTTTTTTTCACTCTCAACTAACATGTCATTACAGGGTGTTTGGAAGAATTAGATATCACAAAAAAAAACCATTCTGTTGAAACTCAACCAGAAACATAACCTCAGATTAGGTGCTAAAACAATCATATTGAGATCCCAGCAATATCACAGTTTGTGTTGAAACTTGAAACCTTACAATTTAGTTATAAATGTTCACAAGAAACAGAAATCAGATGCTCCTGTTCACAGGTTCAAGTGCCTCTACAGTAACCACACTATTTCCACGGATTACCACCATCCCTATATCATTTTTCTCATTGCCATTGACCTCAACAGTATTGTCAACAACCAAATTCATAAACTGGTCAAACCCACGAAGAGTACCAACAATCATACGATTTGCATTCAGCTTGATTTGAAGCTGTTTGTCCATGTATTTCTTCAAATCCGGCGGCTGACCTGACCTGCTCATGGTGAAGAATGGAGATCAGAGAACGCAGAAGGAAGAAGAAGAAGATGATGATTTGAAGCCCCCGAC
TARGET=739,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03278 Ps_contig_mira-and-tgicl-ass_franssen_29368
SEQUENCE=ATGACGGGGAATGATAATTGAAAGGAATGAGAAAAATATAACNACACAACACATTATCTTCGTGACCATATTTCATTCTACCAAGAAGAGAGATGGGAACCACCTCCCTCTCTTCAAACTCACCTTCTCTCATCAACTTCATTCTCACTAGACGTTCAACTTCACTACCATCAACCAGACACAACAATTTCCTCACACTTCAACCTCACACTTCTCTCCCTTTTCATTCCATCACATGCAGCAGCAGCAGCGGCAACAGCAACGCTGCCGAAACAGACCAGGATGAAACAAACTCAATTCAAGCACCAACTACAACACCGGCTAAACTGGTTGAGCTAAGGCTCAGGAGAAGAACAAGGAGACAAGCGAAACGGCAGAGGGAGAAAGGAACTGTTACAAATAGCATAGAGCAACCCAGTAAGGCAGATGCTCCTCCTAAGAAATGGGAGGAAATGAGTTTTCAAGAGAAGGCAATGGAGCTTTATGTGGGAGAGAAAGGTGCTCTGTTTTGGCTTAATAAATTTGCATATGCTTCGATCTATATCATGATTGGAGCTTGGATAGTGTTTAGGTTTGTGGGTCCTGCACTTAATATTTATCAGTTGGATGCACCTGTACTGACACCTAGTGATGTATTGAAGGGATAACAAGTTTGTATTGTAATTTTAACATATGTATTAAACTTTTTTAAGATCACAAAAATCTATCTATTTTTTTAAATGAGTTGTAATGATAAAGTCTTATATGTTTCTTTGTTCAATAGATTAATTTATATTTAGCAATGTAG
TARGET=238,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1316
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.071684
PRIMER_LEFT_SEQUENCE=CTCTCCCTTTTCATTCCATCAC
PRIMER_RIGHT_SEQUENCE=GTTTCGCTTGTCTCCTTGTTCT
PRIMER_LEFT=213,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=59.928
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=159
PRIMER_PAIR_PENALTY_1=0.1824
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=0.122569
PRIMER_LEFT_1_SEQUENCE=CTCTCCCTTTTCATTCCATCAC
PRIMER_RIGHT_1_SEQUENCE=TTAGGAGGAGCATCTGCCTTAC
PRIMER_LEFT_1=213,22
PRIMER_RIGHT_1=438,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=59.877
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.1841
PRIMER_LEFT_2_PENALTY=0.110131
PRIMER_RIGHT_2_PENALTY=0.073989
PRIMER_LEFT_2_SEQUENCE=CAAGAAGAGAGATGGGAACCAC
PRIMER_RIGHT_2_SEQUENCE=GGTGTTGTAGTTGGTGCTTGAA
PRIMER_LEFT_2=81,22
PRIMER_RIGHT_2=318,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.1902
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=CTCTCCCTTTTCATTCCATCAC
PRIMER_RIGHT_3_SEQUENCE=AGCACCTTTCTCTCCCACATAA
PRIMER_LEFT_3=213,22
PRIMER_RIGHT_3=502,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.1916
PRIMER_LEFT_4_PENALTY=0.059875
PRIMER_RIGHT_4_PENALTY=0.131699
PRIMER_LEFT_4_SEQUENCE=CTCTCCCTTTTCATTCCATCAC
PRIMER_RIGHT_4_SEQUENCE=TCTCCTTGTTCTTCTCCTGAGC
PRIMER_LEFT_4=213,22
PRIMER_RIGHT_4=361,22
PRIMER_LEFT_4_TM=59.940
PRIMER_RIGHT_4_TM=60.132
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_03279 Ps_contig_mira-and-tgicl-ass_franssen_29375
SEQUENCE=GGGGATAGTAAAAACCAAGTTCGCTACCACTCTTGAAGAATCTGTGTCAATCCCAACATAGTATTCATCTATGGCAGCAACAATGGCAATGATGATCAACTCCAAGTGTCTAAACACAGCCATACCCAAGTTACAAAACACATCTACCACAGCGAAACTAACAACAACAACATGCAAAACCACCACCGTTCAAAACCTACCAAAGGCTCTTACCTCAGTTGACAACACCAACGTTGTTGTGTCACCTTCCGTTGCAATTGCAGGGGCAATATTCTCATCCCTCGCAACCTGCGATGCCGCTTTCGCTGCTCAGCAAATAGCAGAAATAGCGGAAGGTGATAACAGAGGATTAGCACTGTTGTTGCCATTGGTTCCAGCCATAGGGTGGGTTTTGTTCAACATATTGCAGCCAGCTTTGAACCAAATCAACCGTATGCGTAGCACAAGGGGAGTGATTGTTGGGCTTGGACTCGGGCTTGGCGGGCTTGCGGTATCGGGTATGGTGTCAGAAGCTTCTGCTAGTGAGATTGGTATGATTGCTGAGGCTGCTGGAAGTGATAACAGGGGAAAACAAACTTAACTAAGACTAGTTTAGTGGTTGCATCGGCTATACTTTGGGTGCTTTACAATATTTTGCAGCCTGCTCTTAACCAGATCAATAAGATGAGATCTGAGTAAAATAGGGGTGGGTGGTTTTAGTGCAATTGTTTTGTTGTAACACTTT
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1407
PRIMER_LEFT_PENALTY=0.080323
PRIMER_RIGHT_PENALTY=0.060408
PRIMER_LEFT_SEQUENCE=CACAGCCATACCCAAGTTACAA
PRIMER_RIGHT_SEQUENCE=CAATGGCAACAACAGTGCTAAT
PRIMER_LEFT=114,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.060
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=256
PRIMER_PAIR_PENALTY_1=0.1561
PRIMER_LEFT_1_PENALTY=0.095684
PRIMER_RIGHT_1_PENALTY=0.060408
PRIMER_LEFT_1_SEQUENCE=ACAAAACACATCTACCACAGCG
PRIMER_RIGHT_1_SEQUENCE=CAATGGCAACAACAGTGCTAAT
PRIMER_LEFT_1=132,22
PRIMER_RIGHT_1=369,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.060
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.2063
PRIMER_LEFT_2_PENALTY=0.095684
PRIMER_RIGHT_2_PENALTY=0.110660
PRIMER_LEFT_2_SEQUENCE=ACAAAACACATCTACCACAGCG
PRIMER_RIGHT_2_SEQUENCE=AACGTTGGTGTTGTCAACTGAG
PRIMER_LEFT_2=132,22
PRIMER_RIGHT_2=234,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.2239
PRIMER_LEFT_3_PENALTY=0.080323
PRIMER_RIGHT_3_PENALTY=0.143555
PRIMER_LEFT_3_SEQUENCE=CACAGCCATACCCAAGTTACAA
PRIMER_RIGHT_3_SEQUENCE=ATATGTTGAACAAAACCCACCC
PRIMER_LEFT_3=114,22
PRIMER_RIGHT_3=403,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.2392
PRIMER_LEFT_4_PENALTY=0.095684
PRIMER_RIGHT_4_PENALTY=0.143555
PRIMER_LEFT_4_SEQUENCE=ACAAAACACATCTACCACAGCG
PRIMER_RIGHT_4_SEQUENCE=ATATGTTGAACAAAACCCACCC
PRIMER_LEFT_4=132,22
PRIMER_RIGHT_4=403,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=59.856
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_03280 Ps_contig_mira-and-tgicl-ass_franssen_29376
SEQUENCE=GGGGATCTATATTCCAAACAAAGTCTAGTTCCCAGTCGGGGAATAGTAAAAACCAAGTTCGCTACCACTCTTGAAGAATCTGTGTCAATCCCAACATAGTATTCATCTATGGCAGCAACAATGGCAATGATGATCAACTCCAAGTGTCTAAACACAGCCATACCCAAGTTACAAAACACATCTACCACAGCGAAACTAACAACAACAACAACATGCAAAACCACCACCGTTCAAAACCTACCAAAGGCTATTACCTCATTTGACAACACCAACGTTGTTGTGTCACCTTCCATTGCAATTGCAGGGGCAATATTCTCATCCCTCGCAACCTGCGATGCCGCTTTCGCTGCTCAGCAAATAGCAGAAATAGCGGAAGGTGATAATAGAGGATTAGCACTGTTGTTGCCATTGGTTCCAGCCATAGGGTGGGTTTTGTTCAACATATTGCAGCCAGCTTTGAACCAAATCAACCGTATGCGTAGCACAAGGGGAGTGATTGTTGGGCTTGGACTCGGGCTTGGCGGGCTTGCGGTATCGGGTATGGTGTCAGAAGCTTCTGCTAGTGAGATTGGTATGATTGCTGAGGCTGCTGGAAGTGATAACAGGGGAACACTTCTGCTGTTTGTGGTTGCACCGGCTATACTTTGGGTGCTTTACAATATTTTGCAGCCTGCTCTTAACCAGATCAATAAGATGAGATCTGAGTAAAATAGGGGTGGGTGGTTTTAGTGCAATGTTTTATTGTAACACTTTCTTGTAACTTGTATCTAATGCTCTTGCTTTCTCTATATTGTGTTATATATCAAACTTGGTTTTCTATAGCAGACATTTTATTTTCTGAAACNTT
TARGET=198,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1407
PRIMER_LEFT_PENALTY=0.080323
PRIMER_RIGHT_PENALTY=0.060408
PRIMER_LEFT_SEQUENCE=CACAGCCATACCCAAGTTACAA
PRIMER_RIGHT_SEQUENCE=CAATGGCAACAACAGTGCTAAT
PRIMER_LEFT=152,22
PRIMER_RIGHT=410,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.060
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=0.1561
PRIMER_LEFT_1_PENALTY=0.095684
PRIMER_RIGHT_1_PENALTY=0.060408
PRIMER_LEFT_1_SEQUENCE=ACAAAACACATCTACCACAGCG
PRIMER_RIGHT_1_SEQUENCE=CAATGGCAACAACAGTGCTAAT
PRIMER_LEFT_1=170,22
PRIMER_RIGHT_1=410,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.060
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.2239
PRIMER_LEFT_2_PENALTY=0.080323
PRIMER_RIGHT_2_PENALTY=0.143555
PRIMER_LEFT_2_SEQUENCE=CACAGCCATACCCAAGTTACAA
PRIMER_RIGHT_2_SEQUENCE=ATATGTTGAACAAAACCCACCC
PRIMER_LEFT_2=152,22
PRIMER_RIGHT_2=444,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=59.856
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.2392
PRIMER_LEFT_3_PENALTY=0.095684
PRIMER_RIGHT_3_PENALTY=0.143555
PRIMER_LEFT_3_SEQUENCE=ACAAAACACATCTACCACAGCG
PRIMER_RIGHT_3_SEQUENCE=ATATGTTGAACAAAACCCACCC
PRIMER_LEFT_3=170,22
PRIMER_RIGHT_3=444,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=0.3021
PRIMER_LEFT_4_PENALTY=0.080323
PRIMER_RIGHT_4_PENALTY=0.221785
PRIMER_LEFT_4_SEQUENCE=CACAGCCATACCCAAGTTACAA
PRIMER_RIGHT_4_SEQUENCE=CACCTTCCGCTATTTCTGCTAT
PRIMER_LEFT_4=152,22
PRIMER_RIGHT_4=378,22
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=59.778
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_03281 Ps_contig_mira-and-tgicl-ass_franssen_29377
SEQUENCE=AAGATATCATTGATGAAGCTGTTCATGATCCCCATAGCTTTGCTGGAGATTCCAATGTCAGGATGAACTTGCTTAAGAACCTTGAAGATGTAGATCTTGTACGTCTCAACGCTCTTCTTGTTTCTCTTCTTCTTCTTGTCGGTGGAGGAACGTCCTGGNTCTTTTCTTCCTACGTCCGTTTACGGTTTTACCGTTCCGTTACCGGGTTCCGTTTCCTTTCCTTCCCGTTCCGTTTCGGGTTTTTTTTCCGTTTTCCGTTTCCCCCGTTTTACCGGGGGTCCCGTACGTTGGGTAGTTAGGGTAAGTTAAGGGTAAAAAAAGGTTAAAGTTTTTAGGTAAAGTTAAACCGGGTAAAA
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5080
PRIMER_LEFT_PENALTY=0.327533
PRIMER_RIGHT_PENALTY=0.180440
PRIMER_LEFT_SEQUENCE=CTGGAGATTCCAATGTCAGGAT
PRIMER_RIGHT_SEQUENCE=CTAACTACCCAACGTACGGGAC
PRIMER_LEFT=42,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=60.328
PRIMER_RIGHT_TM=59.820
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.5080
PRIMER_LEFT_1_PENALTY=0.327533
PRIMER_RIGHT_1_PENALTY=0.180440
PRIMER_LEFT_1_SEQUENCE=CTGGAGATTCCAATGTCAGGAT
PRIMER_RIGHT_1_SEQUENCE=ACGGACGTAGGAAGAAAAGANC
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=178,22
PRIMER_LEFT_1_TM=60.328
PRIMER_RIGHT_1_TM=59.820
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=7.0500
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=0.5137
PRIMER_LEFT_2_PENALTY=0.327533
PRIMER_RIGHT_2_PENALTY=0.186184
PRIMER_LEFT_2_SEQUENCE=CTGGAGATTCCAATGTCAGGAT
PRIMER_RIGHT_2_SEQUENCE=ACGTAGGAAGAAAAGANCCAGG
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=174,22
PRIMER_LEFT_2_TM=60.328
PRIMER_RIGHT_2_TM=59.814
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_2=133
PRIMER_PAIR_PENALTY_3=0.5204
PRIMER_LEFT_3_PENALTY=0.340007
PRIMER_RIGHT_3_PENALTY=0.180440
PRIMER_LEFT_3_SEQUENCE=CTGTTCATGATCCCCATAGCTT
PRIMER_RIGHT_3_SEQUENCE=ACGGACGTAGGAAGAAAAGANC
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=178,22
PRIMER_LEFT_3_TM=60.340
PRIMER_RIGHT_3_TM=59.820
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0500
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=0.5204
PRIMER_LEFT_4_PENALTY=0.340007
PRIMER_RIGHT_4_PENALTY=0.180440
PRIMER_LEFT_4_SEQUENCE=AAGCTGTTCATGATCCCCATAG
PRIMER_RIGHT_4_SEQUENCE=ACGGACGTAGGAAGAAAAGANC
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=60.340
PRIMER_RIGHT_4_TM=59.820
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=7.0500
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=164
=
PRIMER_SEQUENCE_ID=SSR_03282 Ps_contig_mira-and-tgicl-ass_franssen_29378
SEQUENCE=GCGGGACAATTCAAATACTAATCTCAATCTCAGTTAAAACTTTCCTTCTTAACTTTCAATTTCGTTTCACACTTTTCTGTCATCCATGGCGCCCAAGGGAGAGAAGAAGCCAGCGGAAAAGAAGCCCGCAGAGAAGGCACCAGCCGCCAAAGCCGAGAAGAAGATCCCAAAGGACGCTTCCTCCACCGACAAGAAGAAGAAGAGAAGCAAGAAGAGCGTTGAGACCTACAAGATCTACATCTTCAAGGTTCTTAAGCAAGTTCATCCTGACATCGGAATCTCCAGCAAGACTATGGGAATCATGAACAGCTTCATCAATGACATCTTCGAGAAGCTCGCTCAGGAATCTTCTAGCCTTGCTCGTTACAGCAAGAAGCATACCATCTCTTCTCGGGAGATTCAGGCTGCCGTTCGCCTTGTTCTTCCCGGCGAGCTTGCTAAGCACGCCGTTTCTGAGGGAACTAAGGCTGTTACGAAATTTACCAGCACTTGAGTTCAAATGTTTTTCCAATTTATCTAATTTATGGTTTATGTAAAGTTGATTTTGGATATTGTTGTATTCATGTAACAATTAAGATATCTAATGAAACTTCATTTTGATCTAAACTTCTTTTTGAAA
TARGET=191,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3821
PRIMER_LEFT_PENALTY=1.301100
PRIMER_RIGHT_PENALTY=0.080962
PRIMER_LEFT_SEQUENCE=AGAAGAAGATCCCAAAGGACG
PRIMER_RIGHT_SEQUENCE=TGATGAAGCTGTTCATGATTCC
PRIMER_LEFT=155,21
PRIMER_RIGHT=316,22
PRIMER_LEFT_TM=59.699
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=162
PRIMER_PAIR_PENALTY_1=1.3967
PRIMER_LEFT_1_PENALTY=1.301100
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=AGAAGAAGATCCCAAAGGACG
PRIMER_RIGHT_1_SEQUENCE=TCCCATAGTCTTGCTGGAGATT
PRIMER_LEFT_1=155,21
PRIMER_RIGHT_1=297,22
PRIMER_LEFT_1_TM=59.699
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=1.3967
PRIMER_LEFT_2_PENALTY=1.301100
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=AGAAGAAGATCCCAAAGGACG
PRIMER_RIGHT_2_SEQUENCE=ATTCCCATAGTCTTGCTGGAGA
PRIMER_LEFT_2=155,21
PRIMER_RIGHT_2=299,22
PRIMER_LEFT_2_TM=59.699
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=1.3967
PRIMER_LEFT_3_PENALTY=1.301100
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=AGAAGAAGATCCCAAAGGACG
PRIMER_RIGHT_3_SEQUENCE=GATTCCCATAGTCTTGCTGGAG
PRIMER_LEFT_3=155,21
PRIMER_RIGHT_3=300,22
PRIMER_LEFT_3_TM=59.699
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=1.3967
PRIMER_LEFT_4_PENALTY=1.301100
PRIMER_RIGHT_4_PENALTY=0.095574
PRIMER_LEFT_4_SEQUENCE=AGAAGAAGATCCCAAAGGACG
PRIMER_RIGHT_4_SEQUENCE=TTCCCATAGTCTTGCTGGAGAT
PRIMER_LEFT_4=155,21
PRIMER_RIGHT_4=298,22
PRIMER_LEFT_4_TM=59.699
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_03283 Ps_contig_mira-and-tgicl-ass_franssen_29379
SEQUENCE=TACGNCGGGGATCACTTGCAATTATCTCTTTCTCATTTTCAATCATTCTTCCAGAAACCCTTTTCTTTCGTTTCACTCTTTTCATCTGCCATGGCACCCAAGGGAGAGAAGAAACCAGCGGCAAAGAAGCCTGCAGAGAAGGCACCAGTAGAACCCAAGGCCGAGAAAAAGATCCCGAAGGATTCATCCTCCACCGACAAGAAGAAGAAGAGAAACAAGAAGAGCGTTGAGACCTACAAGATCTACATCTTCAAGGTTCTTAAGCAAGTTCATCCTGATATTGGAATCTCCAGCAAAGCCATGGGGATCATGAACAGCTTCATCAATGATATCTTCGAGAAGCTCGCTCAGGAGTCTTCTAGACTCTCTCGCTACAACAAAAAATCCACCATCTCTTCCCGGGAGATCCAGACTGCTGTTCGTCTTGTTCTTCCCGGTGAGCTTGCCAAGCACGCCGTTTCCGAGGGAACCAAATCTGTCACCAAGTTCACCAGTTCTGATTAGAAATACTGATTTAAATTAGGGTTTACTTGATGTATATTTTGCATCGGTTCGTTTGTAACGCAGCAATGAAAAATCAATGAAATGAATTGTAGTTTAAAAATTTTTYTAAAAACC
TARGET=199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03284 Ps_contig_mira-and-tgicl-ass_franssen_29383
SEQUENCE=AAGCAGTGGTTACTACAACCGCAGAGTACGNCGGGGACAACTCAATGATCTTTTCCTTCTCTTCTTTCTTTTCTCATCATCTTTTCCAAACTCCATTTTTTTACCTTCAACTTTTCCCATATTAACTTTTTATTTCTTTCATGGAAGAACAACAGAATCATTCTTCTAGAACCTTTTCTTTCGAAAGAAGAAAACTTCCCAACTTTTTAATATCTGTGAAGCTTAAATATGTGAAACTTGGTTACCACTATTTAATTTCCAATGCTATGTACTTAATTTTAATACCTCTCTTAGGAGTAACTTCAGCTCATTTATCAACTATTTCGATCAAAGATTTAATCCAACTCTATGAAACTCTCAAGTTCAACTTTCTTTCAATGACACTATGTTCAAGTTTAGTAGTATTTCTCGCAACGCTGTACTTCATGTCACGTCCAAGAGGCGTATACTTGGTTGATTTTGCATGTTATAAACCGCAACAAGATTTAACTTGCACACGAGAAGTTTTTGTCGAGAAATCGAATTTAACAAAAGCTTTTACAGAAGAGAATCTTTTGTTTCAGAAGAAGATACTTGAGAGATCAGGGTTAGGGCAAAAGACATATTTGCCAAAAGCAATATTGAGGGTCCCACCAAATCCTTGTATGGCTGAAGCAAGGAAAGAAGCTGAAGAGGTTATGTTTGGTGCTATTGATGAAGTGCTTGAGAAAACTGGTGTGAAGGCTAAGGATATCGGAATTTTGGTTGTGAATTGTAGCTTGTTTAATCCAACACCTTCTTTGTCAGCTATGATTGTGAATCATTATAATTTGAGAGGGAATGTTCTAAGCTATAATCTCGGTGGTATGGGCTGTAGTGCCGGACTTATCTCTATCGATCTTGCCAAACAACTTCTTCAGGTACATCCAAACTCATATGCCTTAGTAGTGAGCATGGAGAACATAACCCTAAACTGGTATTTCGGCAACAACAGATCAATGTTAGTCTCAAACTGTCTCTTCAGAATGGGAGGAGCAGCAATCCTCCTCTCCAACAAACCCTCTGACCGCGGTCGAGCGAAATACCAACTAGTCCACACGGTCCGAACGCACAAAGGCGCGGACGACAAATCCTACGGCTGCGTCTTTCAAGAAGAAGACGAAACGAAAAGAGTCGGCGTCGCACTCTCAAAAGACCTAATGGCTGTCGCCGGAGAAGCACTCAAAACAAACATCACAACATTGGGACCATTGGTCCTTCCCATGTCAGAACAGCTCTTATTTTTCGTGACGCTGGTTGCGCGGAAAATCTTCAAGATGAAAATAAAACCCTACATTCCCGATTTTAAACTAGCTTTTGAGCATTTTTGTATTCATGCTGGAGGAAGGGCGGTTTTGGATGAACTTGAAAAAAATCTTGACCTAAGTGATTGGCATATGGAACCATCAAGGATGACACTTTATAGATTTGGCAACACTTCTAGTAGTTCTTTATGGTATGAATTGGCTTATACAGAAGCTAAAGGGAGGATCAGAAAAGGTGATAGAACATGGCAAATTGCATTTGGTTCTGGATTTAAGTGTAACAGTGCTGTTTGGAAAGCTTTGAAGACTATTAATCCTGCTAAGGAAAAGAGTCCTTGGATTGATGAGATTCATGAGTTTCCTGTTCATGTGCCTAAAGTGTCAAAAGTTATTGAGTCTTAAGTATCAATATTTTCATGTTTTTTATTTATTTAATCTATGTTAAGGAATGTGTTTGTTTAAATGAAGTACTAGTAACATGTTTATGGTACTTTTGTTATTTCTTAGTAGATGTTGCAAGAGTAGAAAAGAATAACTTTGCAAATTTAATTTGTATTTTGTTTTTTCTTTGAATGTAGATTCAATATTTGGACATGTTGGTCATGTTTNGTTTGTATCAAATAACTTGTTAAATAAGT
TARGET=1706,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2570
PRIMER_LEFT_PENALTY=0.168114
PRIMER_RIGHT_PENALTY=0.088865
PRIMER_LEFT_SEQUENCE=TCCTGTTCATGTGCCTAAAGTG
PRIMER_RIGHT_SEQUENCE=ACNAAACATGACCAACATGTCC
PRIMER_LEFT=1642,22
PRIMER_RIGHT=1893,22
PRIMER_LEFT_TM=60.168
PRIMER_RIGHT_TM=59.911
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.6482
PRIMER_LEFT_1_PENALTY=0.559375
PRIMER_RIGHT_1_PENALTY=0.088865
PRIMER_LEFT_1_SEQUENCE=CATGAGTTTCCTGTTCATGTGC
PRIMER_RIGHT_1_SEQUENCE=ACNAAACATGACCAACATGTCC
PRIMER_LEFT_1=1634,22
PRIMER_RIGHT_1=1893,22
PRIMER_LEFT_1_TM=60.559
PRIMER_RIGHT_1_TM=59.911
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=260
PRIMER_PAIR_PENALTY_2=0.8220
PRIMER_LEFT_2_PENALTY=0.733091
PRIMER_RIGHT_2_PENALTY=0.088865
PRIMER_LEFT_2_SEQUENCE=TTTCCTGTTCATGTGCCTAAAG
PRIMER_RIGHT_2_SEQUENCE=ACNAAACATGACCAACATGTCC
PRIMER_LEFT_2=1640,22
PRIMER_RIGHT_2=1893,22
PRIMER_LEFT_2_TM=59.267
PRIMER_RIGHT_2_TM=59.911
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=0.8442
PRIMER_LEFT_3_PENALTY=0.755297
PRIMER_RIGHT_3_PENALTY=0.088865
PRIMER_LEFT_3_SEQUENCE=TGTTCATGTGCCTAAAGTGTCA
PRIMER_RIGHT_3_SEQUENCE=ACNAAACATGACCAACATGTCC
PRIMER_LEFT_3=1645,22
PRIMER_RIGHT_3=1893,22
PRIMER_LEFT_3_TM=59.245
PRIMER_RIGHT_3_TM=59.911
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.8465
PRIMER_LEFT_4_PENALTY=0.757660
PRIMER_RIGHT_4_PENALTY=0.088865
PRIMER_LEFT_4_SEQUENCE=GAGTTTCCTGTTCATGTGCCTA
PRIMER_RIGHT_4_SEQUENCE=ACNAAACATGACCAACATGTCC
PRIMER_LEFT_4=1637,22
PRIMER_RIGHT_4=1893,22
PRIMER_LEFT_4_TM=59.242
PRIMER_RIGHT_4_TM=59.911
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_03285 Ps_contig_mira-and-tgicl-ass_franssen_29385
SEQUENCE=CCCATAAAAACAAATACCACGTGTCAAGACTAAAGAGGAATGCGTAGGCACAGAACTNRGNARTRGNACCCACCCACCCATTCAGTTATCGATTTCCAATTCCATTTACACAATAACACTGCTGCAACAACTAGTGTTTAGTTTTCTCCGCCAATCAGAATTCGTTATTCCCCTGAAGATGGCGTTACCGGTGGTGGTGGACGCAGAATACCTCAAGCAGATCGAAAAGGCTCGCCGCGATCTCCGAGCACTCATCGCCAACAGAAACTGCGCTCCTCTCATGCTTCGTTTAGCTTGGCACGATGCGGGCACTTACGATGCAACCTCCAAGACCGGTGGACCTAACGGTTCTATCCGGAACGAGGAAGAGTATTCTCACGGCGCTAACAATGGTTTGAAGAAAGCTATTGATTTCTGTGAGGAAGTGAAGGCAAACCATCCTAAAATTTCATATGCAGACCTTTATCAGCTTGCAGGTGTTGTTGCAGTTGAGGTTACTGGGGGTCCTACAGTCAACTTCGTTCCTGGAAGAAGGGATTCAAAAATATGTACAAGAGATGGGCGGCTTCCCGATGCTAAAAAAGGTGAGTCACATCTCCGTGATATCTTTTATCGTATGGGCTTGACTGACAAGGATATTGTTGCGCTGTCTGGGGCACATACACTGGGAAGAGCACATCCAGAGAGATCAGGGTTTGATGGCGCTTGGACAGAGGACCCTCTGAAGTTTGATAACTCATACTTTGAGATACTTTTGGAAGAAGATTCTGCAGGGCTGCTTAAACTTCCAACGGACAGGGCTTTAGTGGATGATAATGAATTTCGCCATTATGTTGAGCTGTATGCGAAGGACGAGGATGCATTTTTTCGAGATTATGCTGAATCACATAAGAAACTTTCAGAGCTTGGCTTTGTGCCAAGTTCAAAGGTGAATGCTCCTAAGGATGCGACCGTACTAGCACAAAGTGCTGTTGGAGTTGTAGTTGCCGCTGCAGTGGTTATCCTCGCTTACTTGTCTGAACATTCCAAAAGAGGGAAGTAGACTTTGATTTGCTCAGCTCTGTTGGATGTCACGTTTGTGTGGCCCATGATGACCGGTATTGAGTTCATTTTTGCAGTTTCAGCCTATGTTTTCTTAGTACCAATAAGAAAAATGCTACTCGCCACAGCTATTGGGTATATGAATAGACTATAAATTCAACTGAATCCTCCATTTAAGAGCAGCATCAGTATTACTTTGTACATACAAATTTCAGTAGTTCCAGTTATGTGCTCCTTTTCTCTTGAAGATCAGAAAAGACGCGCAGTTAAATTGTTGTTAGTTTATGTTATTTGCAAGAAATTCAGAATTTGAAAACATGGATTATACACTAGAATAATTACTATGTGCCTGAGGATCAATAATACTTATATGTCTTTGAAACNCTGTGCTTATAATTGCAACATAATAGTAATTGCAACATTGGATCTTTAATAATTGTACACAACCGGTGATGCACATATAATGCATGAAATCTAATAGTAATTTGAACG
TARGET=68,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03286 Ps_contig_mira-and-tgicl-ass_franssen_29395
SEQUENCE=TCGGGGGAAAGAGAAGTAATAATAAAAAAATGGAAATTGTGGAAGAAGAAAACGGCGGTGCTAAAAGTGCGTGTTATCTTATCCGAAACGCAACGACACCGAAAACAAATACAAAAACCTTTTGTTCCAACGGAGGAAACATAAACCAAAACACTAAACAATCTAATTATCATTATTTATTATTATTACTCAGCGATGCAACCTACTTCTGGCGACGGTGGCGGTGGTTTGAAACGTTTCCGGTCAACTCCGGCGAGCTGGATAGAATCGTTTATTCTCAAAGAAGAAGAAGAGGAGGACGAAAACCAACAACAAGACAATTTCAGTTTCACTCAACTGCTTTCCAATAACGCTGCTGCCGGTCCTTCAACTCCCGATTCCCATCCATATTTACCTGATTATTACCAGTACTCTTCACCGCATACTCTTTCTGCCGCAGATGCCTCTAATAATCCCTTCACACAGGGGGTTGAGGAGAGTGATAATGCATTGGATCTTAAAATAGACAAAATTTTAGAGGATTCGGTTCCTTGCAAGATTCGGGCAAAGCGTGGCTGTGCTACACATCCAAGGAGTATTGCTGAAAGGGTAACTGTTAATACTAATTTGGGATTTGCTTAATCCGAATTGTGTGAATCTTTTCTGTTTTTGGGTTTCTTGTTATTGTGTAGGTTCGGAGGACTCGAATTAGTGACCGCATAAGAAAACTTCAAGAACTTGTGCCAAACATGGATAAGCAAACAAACACTGCAGAGATGTTAGATGAGGCAGTAGCTTATGTCAAGTTTCTACAAAATCAAATTGAGGAACTATCAGAACACCAACAGAGGTGTACATGCGTGATTCGCGAGTAGATTCGTGATCTTGTGATATAGTACCTGGTTGTTTTCTCTTTTGAGTAATCAGATTTTTTTATGTTCTAATGAAGATTAACCATTTCTCAGAAGATTAAGGTTGTGTCACATTTCTGTGCAACAAGTACATATTGGGGGTGCTATTATCAGTTTCATGAGGAATCCACTTTAAATGTAGTTCCTTCTAGTGTGTTATAATTGTATAGAGTTGTAGTGTTTTATAATAACTTATACACAAATCTGCTAAACTTACTAGCTTATTGAGCTTCTTTTTGCTGAGATATAATTAATCATATCTGAGATTCTTCACATGATTAAAAGAATTATGTCAACTGGCTCTATAGATGATACAATTTTGTATTAGGCTTTTTGTTTAATATTTGTTTTGTTT
TARGET=177,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8029
PRIMER_LEFT_PENALTY=0.216800
PRIMER_RIGHT_PENALTY=0.586117
PRIMER_LEFT_SEQUENCE=GTTCCAACGGAGGAAACATAAA
PRIMER_RIGHT_SEQUENCE=GGTTTTCGTCCTCCTCTTCTTC
PRIMER_LEFT=123,22
PRIMER_RIGHT=306,22
PRIMER_LEFT_TM=60.217
PRIMER_RIGHT_TM=60.586
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.8029
PRIMER_LEFT_1_PENALTY=0.216800
PRIMER_RIGHT_1_PENALTY=0.586117
PRIMER_LEFT_1_SEQUENCE=TTCCAACGGAGGAAACATAAAC
PRIMER_RIGHT_1_SEQUENCE=GGTTTTCGTCCTCCTCTTCTTC
PRIMER_LEFT_1=124,22
PRIMER_RIGHT_1=306,22
PRIMER_LEFT_1_TM=60.217
PRIMER_RIGHT_1_TM=60.586
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.8155
PRIMER_LEFT_2_PENALTY=0.216800
PRIMER_RIGHT_2_PENALTY=0.598673
PRIMER_LEFT_2_SEQUENCE=GTTCCAACGGAGGAAACATAAA
PRIMER_RIGHT_2_SEQUENCE=TTGGAAAGCAGTTGAGTGAAAC
PRIMER_LEFT_2=123,22
PRIMER_RIGHT_2=346,22
PRIMER_LEFT_2_TM=60.217
PRIMER_RIGHT_2_TM=59.401
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.8155
PRIMER_LEFT_3_PENALTY=0.216800
PRIMER_RIGHT_3_PENALTY=0.598673
PRIMER_LEFT_3_SEQUENCE=TTCCAACGGAGGAAACATAAAC
PRIMER_RIGHT_3_SEQUENCE=TTGGAAAGCAGTTGAGTGAAAC
PRIMER_LEFT_3=124,22
PRIMER_RIGHT_3=346,22
PRIMER_LEFT_3_TM=60.217
PRIMER_RIGHT_3_TM=59.401
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.8465
PRIMER_LEFT_4_PENALTY=0.247794
PRIMER_RIGHT_4_PENALTY=0.598673
PRIMER_LEFT_4_SEQUENCE=CGGAGGAAACATAAACCAAAAC
PRIMER_RIGHT_4_SEQUENCE=TTGGAAAGCAGTTGAGTGAAAC
PRIMER_LEFT_4=130,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=59.752
PRIMER_RIGHT_4_TM=59.401
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_03287 Ps_contig_mira-and-tgicl-ass_franssen_29395
SEQUENCE=TCGGGGGAAAGAGAAGTAATAATAAAAAAATGGAAATTGTGGAAGAAGAAAACGGCGGTGCTAAAAGTGCGTGTTATCTTATCCGAAACGCAACGACACCGAAAACAAATACAAAAACCTTTTGTTCCAACGGAGGAAACATAAACCAAAACACTAAACAATCTAATTATCATTATTTATTATTATTACTCAGCGATGCAACCTACTTCTGGCGACGGTGGCGGTGGTTTGAAACGTTTCCGGTCAACTCCGGCGAGCTGGATAGAATCGTTTATTCTCAAAGAAGAAGAAGAGGAGGACGAAAACCAACAACAAGACAATTTCAGTTTCACTCAACTGCTTTCCAATAACGCTGCTGCCGGTCCTTCAACTCCCGATTCCCATCCATATTTACCTGATTATTACCAGTACTCTTCACCGCATACTCTTTCTGCCGCAGATGCCTCTAATAATCCCTTCACACAGGGGGTTGAGGAGAGTGATAATGCATTGGATCTTAAAATAGACAAAATTTTAGAGGATTCGGTTCCTTGCAAGATTCGGGCAAAGCGTGGCTGTGCTACACATCCAAGGAGTATTGCTGAAAGGGTAACTGTTAATACTAATTTGGGATTTGCTTAATCCGAATTGTGTGAATCTTTTCTGTTTTTGGGTTTCTTGTTATTGTGTAGGTTCGGAGGACTCGAATTAGTGACCGCATAAGAAAACTTCAAGAACTTGTGCCAAACATGGATAAGCAAACAAACACTGCAGAGATGTTAGATGAGGCAGTAGCTTATGTCAAGTTTCTACAAAATCAAATTGAGGAACTATCAGAACACCAACAGAGGTGTACATGCGTGATTCGCGAGTAGATTCGTGATCTTGTGATATAGTACCTGGTTGTTTTCTCTTTTGAGTAATCAGATTTTTTTATGTTCTAATGAAGATTAACCATTTCTCAGAAGATTAAGGTTGTGTCACATTTCTGTGCAACAAGTACATATTGGGGGTGCTATTATCAGTTTCATGAGGAATCCACTTTAAATGTAGTTCCTTCTAGTGTGTTATAATTGTATAGAGTTGTAGTGTTTTATAATAACTTATACACAAATCTGCTAAACTTACTAGCTTATTGAGCTTCTTTTTGCTGAGATATAATTAATCATATCTGAGATTCTTCACATGATTAAAAGAATTATGTCAACTGGCTCTATAGATGATACAATTTTGTATTAGGCTTTTTGTTTAATATTTGTTTTGTTT
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7419
PRIMER_LEFT_PENALTY=0.710129
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_SEQUENCE=GCATTATCACTCTCCTCAACCC
PRIMER_LEFT=252,22
PRIMER_RIGHT=487,22
PRIMER_LEFT_TM=60.710
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.7784
PRIMER_LEFT_1_PENALTY=0.710129
PRIMER_RIGHT_1_PENALTY=0.068302
PRIMER_LEFT_1_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_1_SEQUENCE=CCCCTGTGTGAAGGGATTATTA
PRIMER_LEFT_1=252,22
PRIMER_RIGHT_1=467,22
PRIMER_LEFT_1_TM=60.710
PRIMER_RIGHT_1_TM=60.068
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=5.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=216
PRIMER_PAIR_PENALTY_2=0.8155
PRIMER_LEFT_2_PENALTY=0.216800
PRIMER_RIGHT_2_PENALTY=0.598673
PRIMER_LEFT_2_SEQUENCE=GTTCCAACGGAGGAAACATAAA
PRIMER_RIGHT_2_SEQUENCE=TTGGAAAGCAGTTGAGTGAAAC
PRIMER_LEFT_2=123,22
PRIMER_RIGHT_2=346,22
PRIMER_LEFT_2_TM=60.217
PRIMER_RIGHT_2_TM=59.401
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.8155
PRIMER_LEFT_3_PENALTY=0.216800
PRIMER_RIGHT_3_PENALTY=0.598673
PRIMER_LEFT_3_SEQUENCE=TTCCAACGGAGGAAACATAAAC
PRIMER_RIGHT_3_SEQUENCE=TTGGAAAGCAGTTGAGTGAAAC
PRIMER_LEFT_3=124,22
PRIMER_RIGHT_3=346,22
PRIMER_LEFT_3_TM=60.217
PRIMER_RIGHT_3_TM=59.401
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.8465
PRIMER_LEFT_4_PENALTY=0.247794
PRIMER_RIGHT_4_PENALTY=0.598673
PRIMER_LEFT_4_SEQUENCE=CGGAGGAAACATAAACCAAAAC
PRIMER_RIGHT_4_SEQUENCE=TTGGAAAGCAGTTGAGTGAAAC
PRIMER_LEFT_4=130,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=59.752
PRIMER_RIGHT_4_TM=59.401
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_03288 Ps_contig_mira-and-tgicl-ass_franssen_29396
SEQUENCE=TTACCGGTCAACTCCGGCGAGCTGGATAGAATCGTTTATTCTCAAAGAAGAAGAAGAGGAGGTGGCAAACCAATCAATAAAGCAAGTAGAGAGTTGAATAACTCAGAGTTGCGTCTGTGGTGCTTGGGTTTAGGTGTGAAGGTGAAGATAAAATGGACTTGATTGAATTATGGGCAATTTTCGGCCCTGGCGTCGCCGGTGCTGTTTTCGGTGCCGGCTGGTGGATCTGGCTCGACGCCGTCGTATGCAGCTCCACAACCGTTCCCTTCCTTCACTATCTCCCTGGAATTTTTGCATCTCTAGCGGGTTTGATGTTCAATTGCGTTAGAAAAGAAGATATTGATTATTCTCCTTATGACGAAGGAGAATGGAGACTGAAGCTGTGGCTCTTCATTGCATATGTCGTGTCCTTTGTTTCTTTAGCTGGATCAGCGGGTTTACTGATCCAAGATTCACTTGACAAATCTGCCCCTTCAGTGTGGGCTGGAGTTGCAGGTGTTTTGCAGTGTGTTTTTGTCTTGATCAGTGGACTGATTTATTGGACTGCTCATCCTGAATAAGATGAATATGACTCGGAGCTTTAAATTATACCAGGTTATGCTGCCTATGTACCATTTTCTTTATTTAATGCGATGATAAATAGTTGGCTTCGTGTAAATCAGTAAATGTAAATTGTGCTAACAATCTTAAGCCGCTGGGTAGATTCATATTATATGTTTTATTCGGTTGATCAAGATCTGCTATCCAAAGTAATTTTAGTCGTGTGGTGGTTTTAACAAAGATGATTTTCTTCATTTTCTTAATATTTTGAACCAAGAGCACTGCCAAAGCAGAAAAGCTCTACT
TARGET=45,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7776
PRIMER_LEFT_PENALTY=0.710129
PRIMER_RIGHT_PENALTY=0.067516
PRIMER_LEFT_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_SEQUENCE=TTCCAGGGAGATAGTGAAGGAA
PRIMER_LEFT=16,22
PRIMER_RIGHT=287,22
PRIMER_LEFT_TM=60.710
PRIMER_RIGHT_TM=60.068
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.8094
PRIMER_LEFT_1_PENALTY=0.710129
PRIMER_RIGHT_1_PENALTY=0.099308
PRIMER_LEFT_1_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_1_SEQUENCE=CTTCACCTTCACACCTAAACCC
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=146,22
PRIMER_LEFT_1_TM=60.710
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=1.0030
PRIMER_LEFT_2_PENALTY=0.710129
PRIMER_RIGHT_2_PENALTY=0.292834
PRIMER_LEFT_2_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_2_SEQUENCE=TCCAGGGAGATAGTGAAGGAAG
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=286,22
PRIMER_LEFT_2_TM=60.710
PRIMER_RIGHT_2_TM=59.707
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=1.1417
PRIMER_LEFT_3_PENALTY=0.710129
PRIMER_RIGHT_3_PENALTY=0.431597
PRIMER_LEFT_3_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_3_SEQUENCE=TGCAAAAATTCCAGGGAGATAG
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=295,22
PRIMER_LEFT_3_TM=60.710
PRIMER_RIGHT_3_TM=60.432
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=1.1417
PRIMER_LEFT_4_PENALTY=0.710129
PRIMER_RIGHT_4_PENALTY=0.431597
PRIMER_LEFT_4_SEQUENCE=GCGAGCTGGATAGAATCGTTTA
PRIMER_RIGHT_4_SEQUENCE=TAGAGATGCAAAAATTCCAGGG
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=60.710
PRIMER_RIGHT_4_TM=60.432
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_03289 Ps_contig_mira-and-tgicl-ass_franssen_29398
SEQUENCE=AATTAAGNNCNGGATTGATTTCTTTGTTTGTCTTTCATTGCAGCAGAACTTGTCTTTGAGACCGGACACTGCCAGTTCATACCTCCAAAAGCCATATGGCGTCAATGGCTGCGATCGGTGTTCTCAAAGTGCCGCCTTCCTCCTCCTCCTCCTCTTCTTCATCTTCTTCTTCATCCAAAGCCATTGCACGCAACCTCTCATTCACTTCATCACAGCTCTGTGGTGATAAGATTTTCACTGTTTCAGGAACAAGAAGAAGTTCAGGTAGAAACCCTTTCATTGTTTCTCCCAAGGCTGTTTCTGATTCCAAAAACTCTCAAACTTGTCTTGATCCAGATGCTAGCCGAAGTGTTCTTGGCATTATACTTGGAGGTGGTGCTGGGACGCGTCTTTATCCGTTGACAAAGAAGCGGGCGAAACCAGCTGTTCCTCTTGGAGCAAACTATAGATTGATTGACATCCCTGTTAGCAACTGCCTAAATAGCAACATATCAAAGATCTATGTCCTCACACAATTCAATTCGGCGTCCTTGAATCGACACTTGTCCCGTGCGTATGCGAGCAACTTGGGTGGCTACAAAAATGAAGGTTTCGTTGAGGTTCTTGCCGCGCAGCAGAGTCCTGAGAATCCAAATTGGTTCCAGGGCACTGCGGATGCGGTGAGGCAATATTTATGGCTTTTTGAAGAGCACAATGTTTTGGAATACTTGGTTCTGGCGGGTGACCATTTGTATCGAATGGATTATGAGAGATTTATACAAGCACACAGGGAAAGTGATGCTGATATCACTGTTGCGTCATTGCCAATGGATGAAGCGCGTGCCACTGCATTCGGTCTAATGAAAATTGATGAAGAGGGGCGTATAGTTGAGTTTTCAGAGAAGCCGAAAGGAGAACAGTTGAAAGCTATGAAGGTTGATACGACTATTTTGGGTCTCGACGACGAGAGAGCTAAGGAAATGCCTTACATTGCTAGCATGGGTATATATGTTGTCAGCAAACATGTGATGCTAGATCTGCTCCGCGACAAGTTTCCTGGTGCAAACGACTTTGGTAGCGAAGTTATTCCTGGTGCGACCGAGCTTGGATTGAGAGTGCAAGCTTATTTATACGATGGATACTGGGAAGACATTGGTACGATTGAGGCTTTCTATAATGCAAATCTGGGAATCACCAAAAAGCCTGTGCCAGATTTTAGTTTCTATGACCGTTCATCTCCAATCTACACCCAACCTCGATATTTGCCTCCCTCTAAGATGCTTGATGCTGATATCACCGATAGTGTTATTGGTGAAGGATGTGTAATTAAGAATTGCAAAATTCACCATTCTGTCGTTGGACTGCGATCTTGCATATCAGAAGGTGCAATCATTGAGGACACGTTGTTAATGGGAGCAGATTATTATGAGACGGATGCTGATAGGAGGTTTTTGGCTGCTAAAGGCGGTGTTCCAATCGGTATTGGTCAAGAATTCTCATATTAAAAGGGCAATCATTGACAAGAATGCTAGAATTGGTGACGATGTCAAGATTATTAACAGCGACAATGTGCAAGAAGCTGCAAGGGAAACGGAAGGTTATTTCATAAAAAGTGGTATTGTCACAGTAATCAAGGATGCATTAATTCCAAGTGGAACTGTCATCTAAGAGCACTCTCTATTACTGTTGCCTCATGCAGCTGTGCAATGCAACAACCCATTTTCACCGCTAGAAGGTGGTAAAAGAGCAGTTCCGCTTCCTCGTTGGTTTTCTGGTGCAATGTTATATTTGGTTCGCGAGTATATAGAGTAGACGGACCCTTYCTGAAGTCGCGATGTAAATTTAATTTTATTCAGTCAAATAAATGCTTCTTTGGTCTGCAGTGTCTGTGATGCATGTTCTTTTGCAGTTTATCAAAGGTGTGGAATGATATCCACAGAAAACAATGAAAAGTGATACAATAAAAGCCAGACACTTAGCTTCTATTGACGC
TARGET=138,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03290 Ps_contig_mira-and-tgicl-ass_franssen_29404
SEQUENCE=CGACAGAGTACNCGGGGCCAACACAAAGCATCACAACTTAGAAATAAATAACTAATAAAAATGAAAGCAATGTGAGCAAATACCATTCCTACATGAGCAACAAATCGTCTCTCTCTCTAACTCAGAGCAACAATGTGCTCTGCAACTTCCGACCCAACCCACAGCCAAATCCCACCGGAGAAAACGGTGGCGGCCGGTCAACGGTGGCGGCGGCGCCTTCTTCTTCGGCGAACCTCTACCTCAGAGCTTAAATTCCAAACACTAACCCTACCTATTCTAACCTCGCGATCACAGATCCGGTCACGAAAACACCAAAAGGTTAACCTAAAGCCTTGGATCGGAAAACCCTAACCATACACAAAAATCTAAACAGAGTTCTTTTAAAACCAAACCACATAAATCTTCTCCTCAAGCCAAAACAAACACAAAAGAAAATATACACCAACATTCAGAGTCAAAACCCTAAGTCTACAAATGAAGCCACAACGACGGAGATTTTAAGCAATTGACATGGGGGTTTTTGTCGGCTTATCAAGACGAACAAGGTGGTGCAAATATTTAAAGCGAAGGAGAAAGAAGAAGTACTTGACCGGAAGTGGGTCGCTGACGACGTCTTCTCCGCGGTCGTTTCCAGATAAGTTCCTTTCTAATAATCCAATTCCCTTCGTCTTACTCTGTTCTTTTTTAACTTTGAGCGCCTTTAGATGGTTGTGTTGATGAAAGATTATTTTGATTTCTTTTTCGTTTCTTGATTCACTGTCCTCTTCAATGTCCTTTATTCTGGGTATTTATGTTCTTTTCATGATGAATTTATTCAGTCTTGAGATAACTTGTTAGGTTTCTTTTTTCTTATCCTCAAATTTCATGGGATTGTTGTTTTGTGGTTTCATTGTTGTTTGAACTAGTTAACGAAACACATTCTTGGGTGTTCGTGGTAGTCTTTTAGGCAAAACATTAACCTGAGCGTTTTGTTAATTATCCTTCAGTTTTTAAGCGCTTATTGAAAAAGCTTTTAACTTAGATTGTCACTTCCTTCGCAGGTCACAAAATCAATTGAGGAAAAGGGAGCGGATGTCTGTTGGTTCTAAGAAGAGGTTGGGATTTTTAGAGTGCTTTGGCCCAAGAGATGACTTTGGCCGCTGGCTTTTTTGGAAATTCCGTCTTGACACTGTTGAACAATGCAGCTACTTTTAATTTTCAAAATTAGTCATTTAAGATGTACTTTGGACTTTTGGAATGTATTGGATAATGTATTAGCCTTTGGATAATTATGTAACTTTGGGAATTATTGGTAACATTTTGGATTAGAATTCATTTCCGTAATTAATCCTTTTTAATCAACTA
TARGET=108,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1125
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=CCATTCCTACATGAGCAACAAA
PRIMER_RIGHT_SEQUENCE=GTGTATGGTTAGGGTTTTCCGA
PRIMER_LEFT=82,22
PRIMER_RIGHT=358,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=277
PRIMER_PAIR_PENALTY_1=0.1194
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=CCATTCCTACATGAGCAACAAA
PRIMER_RIGHT_1_SEQUENCE=TGATCGCGAGGTTAGAATAGGT
PRIMER_LEFT_1=82,22
PRIMER_RIGHT_1=290,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.1194
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.115769
PRIMER_LEFT_2_SEQUENCE=CCATTCCTACATGAGCAACAAA
PRIMER_RIGHT_2_SEQUENCE=GTGATCGCGAGGTTAGAATAGG
PRIMER_LEFT_2=82,22
PRIMER_RIGHT_2=291,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=60.116
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.1898
PRIMER_LEFT_3_PENALTY=0.073989
PRIMER_RIGHT_3_PENALTY=0.115769
PRIMER_LEFT_3_SEQUENCE=CCAACACAAAGCATCACAACTT
PRIMER_RIGHT_3_SEQUENCE=GTGATCGCGAGGTTAGAATAGG
PRIMER_LEFT_3=17,22
PRIMER_RIGHT_3=291,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=60.116
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=0.1898
PRIMER_LEFT_4_PENALTY=0.073989
PRIMER_RIGHT_4_PENALTY=0.115769
PRIMER_LEFT_4_SEQUENCE=CCAACACAAAGCATCACAACTT
PRIMER_RIGHT_4_SEQUENCE=TGATCGCGAGGTTAGAATAGGT
PRIMER_LEFT_4=17,22
PRIMER_RIGHT_4=290,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=60.116
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_03291 Ps_contig_mira-and-tgicl-ass_franssen_29405
SEQUENCE=AAATARGAAATAAATAGTGAGCAAAATATNATTCCTACATGAGCAACAATTCGTCTCTCTCTCTAACCCAGAGCAACGATATGCTCTGCAACTTCCGACCCAACCCACGGCCAAATCCCACCGGAGAAGACGGTGGCGGCCGGCCAACGGCGGCGGCGCCGCCTTCTTCTCCAGCGAACCTCTACCTCACAACTTAAATTCCAAACACTAACCCTACCTATTCTAGCCTCACGATCACAAATCCGGTCACGAAAACACCAAAAGGTTAACCTAAAGCCTTGGATCGGAAAATCTTAACCATACACAAAAATCTAAACAAAGTTCTTTACAAACCAAACCACATAAATCTTCTCCTCAAGCCAAAACAAACACGAAAGCAAATGAACACTAACATTCAGAGTCAAAACCCTAAGTCTACAAATGAAGCCACAACGGCGGAGATTTTTAAGCAATTGACATGGGGGTTTTGGTCGGCTTATCAAGACAAACGAGGTGGTGCAAATATTTAAAGCGAAAGAGAAAGAAGAAGTACTTGACCGGAGGTGGGTCGCCGACAACGTCTTCTCCGCGGCCGTTTTCAGATAAGCTCCTTTCTAATAATCCAACTCCCTTCGTCTTACTCTGTTCTTTTTTAACTTTGAGCTCCTTTAGATGGTTGTGTTGATGAAAGATTATTTTGATCTCTTTTTTGTTTCTTGATTCACTCTCCTTTTCAATGTCCTCTGTTCTGGGTATTTATGTTCTTTTCATGATGAATTTATTCAGTCTTGAGATAACTTGTTAGGTTTCTTTTTTCTTATCCCCAGATTTCATGGGATTGTTGTTTTGTGGTTTCATTGTTGTTTGAACTGGTTAACGGAACGCATTCTTGGGTGTTCGTGGTAGTCTTTTAGGCAAAACATTAACCTGAGCGTTTTGTTAATTATCCTTCATTTTTTAAGCGCTTATTGAAAAAGCTTTTAACTTAGATTGTCACTTCCTTCGCAGGTCACAAAATCAATTGAGGAAAAAAGGAGAAGATGTATCTTGGTTCTAAGAAGAGGTTGGGATTTTTAGAGTGCTTTGGCCCAAGAGATGACGTTGGCCGCTGGCTTTTTGGAAATTCCATCTTCACACTGCTGGACAATGCAGCAACCTTTAATTTTCAAAATTAGTCAATTTAAGATGTACTTTGGACTTTNGRAACTATTATTGGATAATGTATCAGCCTTTGGATAATTATGTAACTTTGGGAATTTTAGNAACATTTGGATTAGAATTCATTCCGTAATTAATCCTTTTAATCA
TARGET=54,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03292 Ps_contig_mira-and-tgicl-ass_franssen_29413
SEQUENCE=AGAAAAATGATGTGAAAATTTGACATGAGATTAATGGAATAAGTTGCTATCGTAATATGATATTCATCTAAGTTACAAAAGGTTATCTAGCTACGACTTGATATGATATTTTACATGGTAACTAAATAAATAAATAGAATTAGAAACAAAGAAGGAACAAATATAGGCATAAAAAAAAAGATGATATACTACATAAGAGAATGATAAATGAGGCAGAGAGTGTCCTAGAGAATAGGACTAGGAGTGAGTGCATTCAACTGGAAGGCCTTGTGGAAATCTCTTTTTATCTGTGCAATAATTGTAAATCATATAGTTCTTCTGCACCCATCTCATCCTCTGCAAACCAGTTGAGTCAAGCTCTTGTGATAGCCATGATGCAGCAGATGGAGGAGAAGAAGATTTGCATGATGATTTTCCACTGCTCCATATACAAGCATTATTTGCATTGAAGTTTCTGTATGAAGCAGTGAATGGAGCTTTGGACCAATCTGTCTTTATAAGTCCACCTCTTGTTGCCCAATCATCAGCATTCCAAAGGCTTGAGTATATCCTCATTGCTTGATTCTTAGGAAATGGAACTCCAATTCTTTCCAAATTCTTGAACTCCCTTATTGGTGTCCCGTCCACAGAAAACACAATACGTTGTGGATTCCATAGAATCGAATACATGTGAAAATCTGCGGTTGGGTCAAACCATAGATGAAATTGTTGCTCTCTATTGCCTTTTCCTTGGCTAAACACATTGGTGTGAACTATGTAAGGATCACCACTCAAATTTCCCAAGAATTCAAAGTCTATCTCATCCCATGCACCTCCTTTTGATGATAGATAATAGGCCGTGACAGTGCCAGCAGAGTTTCCTGGAACAAGTTTAAGCTGCATATCAATTTTGCCAAATAAATATTCATTTTTGGATTGAAAGCCAGAGCCGGAGGCTTTGTCGAGGGAGAGAGTAAGAAGATTGGCGTTGTTGAGTATTTTGGCACGACCATCTCCCCATGTAATTTGAAAATCTTGGTTGAAGTTAGCAGCAACATGAATGAAAAGGAGAGGAATAAGCAGCAGTAGTAATTTGTTGCAAGCCATGATGTTGTGAATAAATAAAATAGTAAGTGGTAAAGTTGGATAGATATCCCCCGACGG
TARGET=123,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03293 Ps_contig_mira-and-tgicl-ass_franssen_29419
SEQUENCE=TTGAAAAGAAAATATACTATAAATAGAAAGTGATTCAATTTGAAAGGAATCTAAAACATAACAACTCTTCACGCTTTATCAAAGTTCACAACAGTAAAACTAAAGAAAACAAACCCAACTTTGTCTCAACCAAAACTGTCAAAAATATAGGAGGACAAAAGAACCAGGAAACAGAACAAGTTAAAACTTGGTTCAAGATCAAGCGTCGGCAATGGTCACCTCAACTTCAACACCAGGTTCAATGGTGATAGAAGTAATCTGCTTAACCACATCTGGGGAACTGTAAAGATCAATGACTCTCTTGTGCACACGGAGTTCAAATCTATCCCAAGTGTTGGTACCTTCCCCACAAGGGGACTTCCTTGTAGTGATGTGAAGAACTTTAGTAGGCATCCTGACCGGTCCCTTGACCCTGAGATGCTTGTCCTTTGCACCACGAACCAAATCAGCACAAACCTTCTCAAGATTTTGGACATGTTTAGAGGAGAGAGTGATCCTAATCTTGTGGATCTGCTCCTGAGAGTCCTCCAAACCAACCTTTGTGGGTTTCGCAGCTGCGTACGCCATTGCTAAGAGTACAACACTTCTTTGTAACAAGATGAGAAATACTGAGAAACTTCCGTGATGGAAATTGGCGGCGGCGGCGAAGAAGAATAGTTGACGACGCTGCAAGCTGCTAGGGTTCCTTCAACTTTTTAGAACAATAAGTGGTGTTTTATTTTTGTGTCCCCCGAC
TARGET=634,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5382
PRIMER_LEFT_PENALTY=0.039879
PRIMER_RIGHT_PENALTY=0.498341
PRIMER_LEFT_SEQUENCE=ACCAAATCAGCACAAACCTTCT
PRIMER_RIGHT_SEQUENCE=GGGGACACAAAAATAAAACACC
PRIMER_LEFT=439,22
PRIMER_RIGHT=730,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=59.502
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=0.5382
PRIMER_LEFT_1_PENALTY=0.039879
PRIMER_RIGHT_1_PENALTY=0.498341
PRIMER_LEFT_1_SEQUENCE=ACCAAATCAGCACAAACCTTCT
PRIMER_RIGHT_1_SEQUENCE=GGGGGACACAAAAATAAAACAC
PRIMER_LEFT_1=439,22
PRIMER_RIGHT_1=731,22
PRIMER_LEFT_1_TM=60.040
PRIMER_RIGHT_1_TM=59.502
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.7361
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.696189
PRIMER_LEFT_2_SEQUENCE=ACCAAATCAGCACAAACCTTCT
PRIMER_RIGHT_2_SEQUENCE=AAAAGTTGAAGGAACCCTAGCA
PRIMER_LEFT_2=439,22
PRIMER_RIGHT_2=695,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=59.304
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.7820
PRIMER_LEFT_3_PENALTY=0.283676
PRIMER_RIGHT_3_PENALTY=0.498341
PRIMER_LEFT_3_SEQUENCE=CAAATCAGCACAAACCTTCTCA
PRIMER_RIGHT_3_SEQUENCE=GGGGACACAAAAATAAAACACC
PRIMER_LEFT_3=441,22
PRIMER_RIGHT_3=730,22
PRIMER_LEFT_3_TM=60.284
PRIMER_RIGHT_3_TM=59.502
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.7820
PRIMER_LEFT_4_PENALTY=0.283676
PRIMER_RIGHT_4_PENALTY=0.498341
PRIMER_LEFT_4_SEQUENCE=CAAATCAGCACAAACCTTCTCA
PRIMER_RIGHT_4_SEQUENCE=GGGGGACACAAAAATAAAACAC
PRIMER_LEFT_4=441,22
PRIMER_RIGHT_4=731,22
PRIMER_LEFT_4_TM=60.284
PRIMER_RIGHT_4_TM=59.502
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_03294 Ps_contig_mira-and-tgicl-ass_franssen_29421
SEQUENCE=AAGTTAAATGAAAATCTGCATTAATAAATGAAAATTACAAATCATTTTAAAATACTATATTTATCAAAGTGACACTCATTGTAAAACAAAATGACTTATGTTTCTTACGTTAAGGACATAAAATAGTTCTTTCTTAAAAATATCAGGATCAACTCATCTGTGACATGATCATATTTGTATATGATTACTGGTATTTGTCGTAAAAATAATCATCAAAAGAAATTTACCATATGTTTCATGATTCTTTTCTTACAATGGATCTTTGATTGTCAGCCGCCCGATTTATTTACGGCTTAGAAATGAAAGGAAGGAAAGTAATGAGGCAATGAGGGAATGTTCTGAAAATAACTTAAAAAGAATAGACCATATTAACGCAGGATACCCGACAACACCGTCAAGTTAGGAGATTGAAAGACCCATCCATAGCAGAAGTCTTCGCGGTCGATCAAGAAACCTGGCCTGCCATGCAGGTTCATCACTCTCGTAAACAAACCCACTCTTCATGTACAATTTCTTTGCTGGTTCATTGTCATCGGCAACATGGACGTATAAATCTGTTATGCCCCAATTACGAGCAACTAACTTCGACTTTTCAAGAAGTTCATAGGCCAATCCATTCCTATGCAACTCTCTAGCAACACACACATTACTCAGGTAAGCCCTTGTCGTATCAGCTCCGCTAACCTCTGGCTTCATTCCTACAATTTCATCCGGAAGACTCGAGCACTGATTAAGATCGAGAGAACCAACTACTATCCTGTCTTCTCCATAAGTAGAAAAATGAAGAACATAAATCATCATAGATGTTAGATATATGAGACACAGGAAGAGAAGCATTTATGCAAGAGACTCTTCTAAAACCCACCCTCTTCCCTGAAATGCGCTCTTTCAATGCTTCAAACTCACGTTCAGCCAAGTACTTCACATGATCCTAACACAATTGGACTCCGTAAGTTCCGGGACGGAATTGATTGAAGGAACGAACACGGAGGGAAGAAGCTGCCCAGAGTTCATCATCGTAGAAAGATTCGGCGATGGTGATCAAAGATGTGTCGATTTTTTGGGTGCAAATGTTTGTGGAAGAAGGTGAGGATTGGACTTGAGCTCTGGTGTTGTAATTAGCGAATGTTAATGCAGAATTTGGAAAGGTAGAAGAAGAAGAGTGAATTGAAGGGAAGGAACAGAGCGAAGAAATTAGCGTCATTTTTTGTTAGGTGTGTTGTTTGTTGTATTATTCGTCGTTCGCATATGCATCTTGTTCTTGTTTTTGAATACCCCGAA
TARGET=1151,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0504
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.026872
PRIMER_LEFT_SEQUENCE=TTTGTGGAAGAAGGTGAGGATT
PRIMER_RIGHT_SEQUENCE=GCGAACGACGAATAATACAACA
PRIMER_LEFT=1073,22
PRIMER_RIGHT=1245,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.027
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.0504
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.026872
PRIMER_LEFT_1_SEQUENCE=TTTGTGGAAGAAGGTGAGGATT
PRIMER_RIGHT_1_SEQUENCE=TGCGAACGACGAATAATACAAC
PRIMER_LEFT_1=1073,22
PRIMER_RIGHT_1=1246,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.027
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=174
PRIMER_PAIR_PENALTY_2=0.0717
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.048131
PRIMER_LEFT_2_SEQUENCE=TTTGTGGAAGAAGGTGAGGATT
PRIMER_RIGHT_2_SEQUENCE=GACGCTAATTTCTTCGCTCTGT
PRIMER_LEFT_2=1073,22
PRIMER_RIGHT_2=1201,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.048
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=129
PRIMER_PAIR_PENALTY_3=0.1334
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.109882
PRIMER_LEFT_3_SEQUENCE=TTTGTGGAAGAAGGTGAGGATT
PRIMER_RIGHT_3_SEQUENCE=AATGACGCTAATTTCTTCGCTC
PRIMER_LEFT_3=1073,22
PRIMER_RIGHT_3=1204,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=59.890
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=0.1370
PRIMER_LEFT_4_PENALTY=0.110131
PRIMER_RIGHT_4_PENALTY=0.026872
PRIMER_LEFT_4_SEQUENCE=GAAGGTGAGGATTGGACTTGAG
PRIMER_RIGHT_4_SEQUENCE=GCGAACGACGAATAATACAACA
PRIMER_LEFT_4=1082,22
PRIMER_RIGHT_4=1245,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=60.027
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=164
=
PRIMER_SEQUENCE_ID=SSR_03295 Ps_contig_mira-and-tgicl-ass_franssen_29422
SEQUENCE=CCATTCCTACTGCAACTCTCTAGCACACACACATTACTCAGGTAAGCCCTTCGTCGTATCAGCTCCGCTAACCTCTGGCTTCATTCCTACAATTTCATCCGGAAGACTCAAGCACTGATTAAGATCGAGAGAACCAACTACTATCCTGTCTTCTCCATAAGTAGAAAACTTACATGAAGAACATAAATCATCATAGATGTTAGATATATGAGACACAGGAAGAGAAGCATTTATGCAAGAGACTCTTCTAAAACCCACCCTCTTCCCTGAAATGCGCTCTTTCAATGCTTCAAACTCACGTTCAGCCAAGTACTTCACATGATCTTGGACTCCGTAAGTTCCGGGACGGAATTGATTGAAGGAACGAACACGGAGGGAAGAAGCTGCCCAGAGTTCATCATCGTAGAAAGATTCGGCGATGGTGATCAAAGATGTGTCGATTTTTTGGGTGCAAATGTTTGTGGAAGAAGGTGNGATTGNTTGGAGTTCTGGTGTTGTAATTTGNGAATGTTAATGCAGAATTTGGAAAGGTAGAAGAAGAAGAG
TARGET=24,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9402
PRIMER_LEFT_PENALTY=2.775601
PRIMER_RIGHT_PENALTY=0.164639
PRIMER_LEFT_SEQUENCE=CCATTCCTACTGCAACTCTCTAGC
PRIMER_RIGHT_SEQUENCE=GTCTTCCGGATGAAATTGTAGG
PRIMER_LEFT=0,24
PRIMER_RIGHT=106,22
PRIMER_LEFT_TM=60.776
PRIMER_RIGHT_TM=59.835
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=3.0802
PRIMER_LEFT_1_PENALTY=2.775601
PRIMER_RIGHT_1_PENALTY=0.304643
PRIMER_LEFT_1_SEQUENCE=CCATTCCTACTGCAACTCTCTAGC
PRIMER_RIGHT_1_SEQUENCE=CTTCCGGATGAAATTGTAGGAA
PRIMER_LEFT_1=0,24
PRIMER_RIGHT_1=104,22
PRIMER_LEFT_1_TM=60.776
PRIMER_RIGHT_1_TM=60.305
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=3.1089
PRIMER_LEFT_2_PENALTY=2.775601
PRIMER_RIGHT_2_PENALTY=0.333282
PRIMER_LEFT_2_SEQUENCE=CCATTCCTACTGCAACTCTCTAGC
PRIMER_RIGHT_2_SEQUENCE=GGAAGAGGGTGGGTTTTAGAAG
PRIMER_LEFT_2=0,24
PRIMER_RIGHT_2=265,22
PRIMER_LEFT_2_TM=60.776
PRIMER_RIGHT_2_TM=60.333
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=3.2793
PRIMER_LEFT_3_PENALTY=2.775601
PRIMER_RIGHT_3_PENALTY=0.503688
PRIMER_LEFT_3_SEQUENCE=CCATTCCTACTGCAACTCTCTAGC
PRIMER_RIGHT_3_SEQUENCE=TGCATAAATGCTTCTCTTCCTG
PRIMER_LEFT_3=0,24
PRIMER_RIGHT_3=236,22
PRIMER_LEFT_3_TM=60.776
PRIMER_RIGHT_3_TM=59.496
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=3.5681
PRIMER_LEFT_4_PENALTY=2.775601
PRIMER_RIGHT_4_PENALTY=0.792480
PRIMER_LEFT_4_SEQUENCE=CCATTCCTACTGCAACTCTCTAGC
PRIMER_RIGHT_4_SEQUENCE=TCTTCCGGATGAAATTGTAGGA
PRIMER_LEFT_4=0,24
PRIMER_RIGHT_4=105,22
PRIMER_LEFT_4_TM=60.776
PRIMER_RIGHT_4_TM=60.792
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=106
=
PRIMER_SEQUENCE_ID=SSR_03296 Ps_contig_mira-and-tgicl-ass_franssen_29422
SEQUENCE=CCATTCCTACTGCAACTCTCTAGCACACACACATTACTCAGGTAAGCCCTTCGTCGTATCAGCTCCGCTAACCTCTGGCTTCATTCCTACAATTTCATCCGGAAGACTCAAGCACTGATTAAGATCGAGAGAACCAACTACTATCCTGTCTTCTCCATAAGTAGAAAACTTACATGAAGAACATAAATCATCATAGATGTTAGATATATGAGACACAGGAAGAGAAGCATTTATGCAAGAGACTCTTCTAAAACCCACCCTCTTCCCTGAAATGCGCTCTTTCAATGCTTCAAACTCACGTTCAGCCAAGTACTTCACATGATCTTGGACTCCGTAAGTTCCGGGACGGAATTGATTGAAGGAACGAACACGGAGGGAAGAAGCTGCCCAGAGTTCATCATCGTAGAAAGATTCGGCGATGGTGATCAAAGATGTGTCGATTTTTTGGGTGCAAATGTTTGTGGAAGAAGGTGNGATTGNTTGGAGTTCTGGTGTTGTAATTTGNGAATGTTAATGCAGAATTTGGAAAGGTAGAAGAAGAAGAG
TARGET=533,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03297 Ps_contig_mira-and-tgicl-ass_franssen_29426
SEQUENCE=CACATTCTCACACTATCATCATCATCAATGCAATTGTAAAAACCATGTCAACTACCATCTCCATGGCTTCTTTCTCTCCTCTAACTCCAATCTCAACCACTCATTCTTCACACTCCAGACCCCATCCCCACAACAATCTCTTCACTCTCACTCTCACACCCAAAAACCTTTCTTTCTTTACCCTCTCTCCCTTGAGAGTTTCTGCTGGAACTTCTGATTCACCTGAAACTGCTGTCGAACCACCGCCACAAAACATCTCTGTCGGTACAAACGGCTCTGCTTCTGCTGTTACCGCGGCAGAAGAAGTTAAGGTTTCCAACGGTTTTCTTGACCCTAGATGGGTAGCTGGGACATGGGACTTGATGCAGTTTAGGAAAAACGGTACCACCGATTGGGATGCTGTTATTGATGCTGAGGCGAGGAGGAGAAAATGGCTTGAGACTAACCCAGAATCATCCAACAATGACAGTCCTGTAGTGTTTGACACCTCTATCGTACCTTGGTGGGCATGGATAAAAAGGTTCCATCTCCCTGAAGCTGAACTACTTAATGGTCGCGCAGCAATGATAGGATTCTTTATGACATATCTGGTCGATAGCTTGACAGGAGTAGGTCTAGTTGATCAAATGAACAACTTCTTTTGCAAAACTCTTCTGTTTGTAGCAGTGGGAGGAGTACTTCTCATCCGAAAGAATGAGGACGTTGATACTTTTAAGAAGCTATTGGAGGAGACTACATTTTATGACAAGCAATGGCAAGCAACTTGGCAGGATGAGAACTCAAGCAATTCCAACAAATGATTAGTGGGATTTGCTGTTATGTAGTCTCTTAATATAGTGTGCTCTATTATTAATCTTGCTAGTGCAGAAAAAGACATGAATATGATTTAGACATTGTATTTCAATTCCTTTTATGATATCCTAATTCAAGAATGATAGAGTTTTTCGCGTTCAATGCAGAATTTTTCTCATGTTAATAAGGAATTGGGTTGTTAGTTTGAG
TARGET=15,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03298 Ps_contig_mira-and-tgicl-ass_franssen_29426
SEQUENCE=CACATTCTCACACTATCATCATCATCAATGCAATTGTAAAAACCATGTCAACTACCATCTCCATGGCTTCTTTCTCTCCTCTAACTCCAATCTCAACCACTCATTCTTCACACTCCAGACCCCATCCCCACAACAATCTCTTCACTCTCACTCTCACACCCAAAAACCTTTCTTTCTTTACCCTCTCTCCCTTGAGAGTTTCTGCTGGAACTTCTGATTCACCTGAAACTGCTGTCGAACCACCGCCACAAAACATCTCTGTCGGTACAAACGGCTCTGCTTCTGCTGTTACCGCGGCAGAAGAAGTTAAGGTTTCCAACGGTTTTCTTGACCCTAGATGGGTAGCTGGGACATGGGACTTGATGCAGTTTAGGAAAAACGGTACCACCGATTGGGATGCTGTTATTGATGCTGAGGCGAGGAGGAGAAAATGGCTTGAGACTAACCCAGAATCATCCAACAATGACAGTCCTGTAGTGTTTGACACCTCTATCGTACCTTGGTGGGCATGGATAAAAAGGTTCCATCTCCCTGAAGCTGAACTACTTAATGGTCGCGCAGCAATGATAGGATTCTTTATGACATATCTGGTCGATAGCTTGACAGGAGTAGGTCTAGTTGATCAAATGAACAACTTCTTTTGCAAAACTCTTCTGTTTGTAGCAGTGGGAGGAGTACTTCTCATCCGAAAGAATGAGGACGTTGATACTTTTAAGAAGCTATTGGAGGAGACTACATTTTATGACAAGCAATGGCAAGCAACTTGGCAGGATGAGAACTCAAGCAATTCCAACAAATGATTAGTGGGATTTGCTGTTATGTAGTCTCTTAATATAGTGTGCTCTATTATTAATCTTGCTAGTGCAGAAAAAGACATGAATATGATTTAGACATTGTATTTCAATTCCTTTTATGATATCCTAATTCAAGAATGATAGAGTTTTTCGCGTTCAATGCAGAATTTTTCTCATGTTAATAAGGAATTGGGTTGTTAGTTTGAG
TARGET=168,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1818
PRIMER_LEFT_PENALTY=0.151533
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=CATCTCCATGGCTTCTTTCTCT
PRIMER_RIGHT_SEQUENCE=TACCGACAGAGATGTTTTGTGG
PRIMER_LEFT=55,22
PRIMER_RIGHT=266,22
PRIMER_LEFT_TM=59.848
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.2445
PRIMER_LEFT_1_PENALTY=0.237561
PRIMER_RIGHT_1_PENALTY=0.006945
PRIMER_LEFT_1_SEQUENCE=CCACTCATTCTTCACACTCCAG
PRIMER_RIGHT_1_SEQUENCE=TGAATCAGAAGTTCCAGCAGAA
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=220,22
PRIMER_LEFT_1_TM=59.762
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=0.2678
PRIMER_LEFT_2_PENALTY=0.237561
PRIMER_RIGHT_2_PENALTY=0.030276
PRIMER_LEFT_2_SEQUENCE=CCACTCATTCTTCACACTCCAG
PRIMER_RIGHT_2_SEQUENCE=TACCGACAGAGATGTTTTGTGG
PRIMER_LEFT_2=96,22
PRIMER_RIGHT_2=266,22
PRIMER_LEFT_2_TM=59.762
PRIMER_RIGHT_2_TM=60.030
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.2955
PRIMER_LEFT_3_PENALTY=0.151533
PRIMER_RIGHT_3_PENALTY=0.143968
PRIMER_LEFT_3_SEQUENCE=CATCTCCATGGCTTCTTTCTCT
PRIMER_RIGHT_3_SEQUENCE=CAGCTACCCATCTAGGGTCAAG
PRIMER_LEFT_3=55,22
PRIMER_RIGHT_3=347,22
PRIMER_LEFT_3_TM=59.848
PRIMER_RIGHT_3_TM=60.144
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.3815
PRIMER_LEFT_4_PENALTY=0.237561
PRIMER_RIGHT_4_PENALTY=0.143968
PRIMER_LEFT_4_SEQUENCE=CCACTCATTCTTCACACTCCAG
PRIMER_RIGHT_4_SEQUENCE=CAGCTACCCATCTAGGGTCAAG
PRIMER_LEFT_4=96,22
PRIMER_RIGHT_4=347,22
PRIMER_LEFT_4_TM=59.762
PRIMER_RIGHT_4_TM=60.144
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_03299 Ps_contig_mira-and-tgicl-ass_franssen_29427
SEQUENCE=TTCGGATGAGAAGTACTCCTCCCACTGCTACAAACAGAAGAGTTTTGCAAAAGAAGTTGTTCATTTGATCAACTAGACCTACTCCTGTCAAGCTATCGACCAGATATGTCATAAAGAATCCTATCATTGCTGCGCGACCATTAAGTAGTTCAGCTTCAGGGAGATGGAACCTTTTTATCCATGCCCACCAAGGTACGATAGAGGTGTCAAACACTACAGGACTGTCATTGTTGGATGATTCTGGGTTACTCTCAAGCCATTTTCTCCTCCTCGCCTCAGCATCAATAACAGCATCCCAATCGGTGGTACCGTTTTTCCTAAACTGCATCAAGTCCCATGTCCCAGCTACCCATCTAGGGTCAACAAAACCGTTGGAAACCTTAACTTCTTCTGCCGCGGTAACAGCAGAAGCAGAGCCGTTTGTACCGACAGAGATGTTTTGTGGCGGTGGTTTGACAGCAGTTTCAGGTGAATCAGAAGTTCCAGCAGAAACTCTCAAGGGAGAGAGGGTAAAGAAAGAAAGGTTTTTGGGTGTGAGAGTGAGAGTGAAGAGATTGTTGTGGGGATGGGGTCTGGAGTGTGAAGAATGAGTGGTTGAGATTGGAGTTAGAGGAGAGAAAGAAGCCATGGAGATGGTAGTTGACATGGTTTTTACAATTGCATTGATGATGATGATAGTGTGAGAATGTGTGGTTTGGGTATTCTCTCTTTATGAGTTTTCAGTTTCTGTTTATGTTTCTTAAGCTATCTATCTATCCTCTCTCATTATCTCATCCTTTCAGAAAGGAACAATACCAAGGGAAGTCAGCAAGGGAAGTGTCTNNCYGANTTAATTAATAAGTTACTATAAAAGCTTTTTTTTTAAGTGAAATAAT
TARGET=512,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03300 Ps_contig_mira-and-tgicl-ass_franssen_29427
SEQUENCE=TTCGGATGAGAAGTACTCCTCCCACTGCTACAAACAGAAGAGTTTTGCAAAAGAAGTTGTTCATTTGATCAACTAGACCTACTCCTGTCAAGCTATCGACCAGATATGTCATAAAGAATCCTATCATTGCTGCGCGACCATTAAGTAGTTCAGCTTCAGGGAGATGGAACCTTTTTATCCATGCCCACCAAGGTACGATAGAGGTGTCAAACACTACAGGACTGTCATTGTTGGATGATTCTGGGTTACTCTCAAGCCATTTTCTCCTCCTCGCCTCAGCATCAATAACAGCATCCCAATCGGTGGTACCGTTTTTCCTAAACTGCATCAAGTCCCATGTCCCAGCTACCCATCTAGGGTCAACAAAACCGTTGGAAACCTTAACTTCTTCTGCCGCGGTAACAGCAGAAGCAGAGCCGTTTGTACCGACAGAGATGTTTTGTGGCGGTGGTTTGACAGCAGTTTCAGGTGAATCAGAAGTTCCAGCAGAAACTCTCAAGGGAGAGAGGGTAAAGAAAGAAAGGTTTTTGGGTGTGAGAGTGAGAGTGAAGAGATTGTTGTGGGGATGGGGTCTGGAGTGTGAAGAATGAGTGGTTGAGATTGGAGTTAGAGGAGAGAAAGAAGCCATGGAGATGGTAGTTGACATGGTTTTTACAATTGCATTGATGATGATGATAGTGTGAGAATGTGTGGTTTGGGTATTCTCTCTTTATGAGTTTTCAGTTTCTGTTTATGTTTCTTAAGCTATCTATCTATCCTCTCTCATTATCTCATCCTTTCAGAAAGGAACAATACCAAGGGAAGTCAGCAAGGGAAGTGTCTNNCYGANTTAATTAATAAGTTACTATAAAAGCTTTTTTTTTAAGTGAAATAAT
TARGET=664,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03301 Ps_contig_mira-and-tgicl-ass_franssen_29427
SEQUENCE=TTCGGATGAGAAGTACTCCTCCCACTGCTACAAACAGAAGAGTTTTGCAAAAGAAGTTGTTCATTTGATCAACTAGACCTACTCCTGTCAAGCTATCGACCAGATATGTCATAAAGAATCCTATCATTGCTGCGCGACCATTAAGTAGTTCAGCTTCAGGGAGATGGAACCTTTTTATCCATGCCCACCAAGGTACGATAGAGGTGTCAAACACTACAGGACTGTCATTGTTGGATGATTCTGGGTTACTCTCAAGCCATTTTCTCCTCCTCGCCTCAGCATCAATAACAGCATCCCAATCGGTGGTACCGTTTTTCCTAAACTGCATCAAGTCCCATGTCCCAGCTACCCATCTAGGGTCAACAAAACCGTTGGAAACCTTAACTTCTTCTGCCGCGGTAACAGCAGAAGCAGAGCCGTTTGTACCGACAGAGATGTTTTGTGGCGGTGGTTTGACAGCAGTTTCAGGTGAATCAGAAGTTCCAGCAGAAACTCTCAAGGGAGAGAGGGTAAAGAAAGAAAGGTTTTTGGGTGTGAGAGTGAGAGTGAAGAGATTGTTGTGGGGATGGGGTCTGGAGTGTGAAGAATGAGTGGTTGAGATTGGAGTTAGAGGAGAGAAAGAAGCCATGGAGATGGTAGTTGACATGGTTTTTACAATTGCATTGATGATGATGATAGTGTGAGAATGTGTGGTTTGGGTATTCTCTCTTTATGAGTTTTCAGTTTCTGTTTATGTTTCTTAAGCTATCTATCTATCCTCTCTCATTATCTCATCCTTTCAGAAAGGAACAATACCAAGGGAAGTCAGCAAGGGAAGTGTCTNNCYGANTTAATTAATAAGTTACTATAAAAGCTTTTTTTTTAAGTGAAATAAT
TARGET=745,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03302 Ps_contig_mira-and-tgicl-ass_franssen_29441
SEQUENCE=AAGAAGGCGAGGCCGGCCCCAAAACTAGCAACTGCTGCTTAAATTTTGTATTAAAATTAATTAATTGTCTAGCAAATTTAAGTTATTTATTAAATAGAGCGCGGTTTTTGTTTGTAAGTGACTGAGCAGAGTTTGGTTTTCTATTGTACTAGTGAATTTCTGTACTGTCTTCATGTTATTTCAGTTTAATTTAATTACCTTATGAATGTTATGGTTAGATAANTTCTTTTTAGTTTGAATGTGTGTTGTTGTGATTGTTAACTTGGTCATCCTTGACAATAAACTNTNTCATTAGAAACT
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.8662
PRIMER_LEFT_PENALTY=3.894971
PRIMER_RIGHT_PENALTY=0.971278
PRIMER_LEFT_SEQUENCE=GGCCCCAAAACTAGCAACTG
PRIMER_RIGHT_SEQUENCE=AAAACCAAACTCTGCTCAGTCA
PRIMER_LEFT=14,20
PRIMER_RIGHT=139,22
PRIMER_LEFT_TM=61.895
PRIMER_RIGHT_TM=59.029
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=126
PRIMER_PAIR_PENALTY_1=5.0493
PRIMER_LEFT_1_PENALTY=3.894971
PRIMER_RIGHT_1_PENALTY=1.154341
PRIMER_LEFT_1_SEQUENCE=GGCCCCAAAACTAGCAACTG
PRIMER_RIGHT_1_SEQUENCE=AAAACCAAACTCTGCTCAGTCAC
PRIMER_LEFT_1=14,20
PRIMER_RIGHT_1=139,23
PRIMER_LEFT_1_TM=61.895
PRIMER_RIGHT_1_TM=59.846
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=5.1719
PRIMER_LEFT_2_PENALTY=3.894971
PRIMER_RIGHT_2_PENALTY=1.276904
PRIMER_LEFT_2_SEQUENCE=GGCCCCAAAACTAGCAACTG
PRIMER_RIGHT_2_SEQUENCE=GTCACTTACAAACAAAAACCGC
PRIMER_LEFT_2=14,20
PRIMER_RIGHT_2=121,22
PRIMER_LEFT_2_TM=61.895
PRIMER_RIGHT_2_TM=58.723
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=11.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=5.3874
PRIMER_LEFT_3_PENALTY=3.894971
PRIMER_RIGHT_3_PENALTY=1.492430
PRIMER_LEFT_3_SEQUENCE=GGCCCCAAAACTAGCAACTG
PRIMER_RIGHT_3_SEQUENCE=AAACCAAACTCTGCTCAGTCAC
PRIMER_LEFT_3=14,20
PRIMER_RIGHT_3=138,22
PRIMER_LEFT_3_TM=61.895
PRIMER_RIGHT_3_TM=58.508
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=5.4075
PRIMER_LEFT_4_PENALTY=3.894971
PRIMER_RIGHT_4_PENALTY=1.512483
PRIMER_LEFT_4_SEQUENCE=GGCCCCAAAACTAGCAACTG
PRIMER_RIGHT_4_SEQUENCE=AACCAAACTCTGCTCAGTCACTT
PRIMER_LEFT_4=14,20
PRIMER_RIGHT_4=137,23
PRIMER_LEFT_4_TM=61.895
PRIMER_RIGHT_4_TM=59.488
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_03303 Ps_contig_mira-and-tgicl-ass_franssen_29450
SEQUENCE=AAAAGTTTTTTAAATTGATAATAATAATAATAATCTACTGTAGTAGTTCAGTTAAGCAATGATATCATAGACAGCAAAAGTACAAATCATTTATTCATTAATTTTCATAATATAAACTAACATTCAGTACAAAGGGCAAATATCAGAATTATTACATAATATAATATAGAAGTTACAATGAGAAGTTCAGAATATATGAAGCCAAGAGATAAAAAATCCAGAGCTTTGGTAGAAAAAACAAATCCTTCTCCAAAGAAAAGGATTGTTCATGACAGAACTATATAGTTTCTGCCATGGCCAAAACCCTAGAGAAAACAAAAGGCCTCATATGACTCTTAACTGCCAATAGTAACCAAGATAACAATATTGCGGAAATCGATCTTCAACATGCCCCTTGACTAAAACTGCGACACCAAACATCGTGTATAGAACGTAGTTCAAATACACGAAAAATCGGGTAAACACCAACCACTCTGGACGAACTTTGGTACAGACTGCTGTGATGAAATTGAAAATGACAAAACATGGTGGCAAAACCGATACCAAGCGGAGCTAAAGACATCAGGTGCCAACATCAGAAAGAAACCTAAAATCCAGTACCCGGTAGCTACGTTCACAGAGATAAACAGCTGTTCTAAAAGCGACTTCCTAAGAATAGGATGACCAAAAAAATTCTAACAGATTTCCTTCTAATTTTAAGGAAACCTGTAAAAACAGATATTGAACAACTGCTTGCTCTGGAAAAAGAGAACCGAGTACTGCGGTGACCAAGACCCACCGATAATTAATGGACCCAAAAACACTACCATGCAAGACTGATTATAAAACCTGGAGTGCTTGCTATCCAGATATGCAGATATATGGATATCAAGGCTACCTCCTCAGAAGAGGATGTCACATATCCAGAGACTTTCAATAAGCTTGTCAACTGTGTGGCTGCCGATCGACTTGCAATTTTTGATATTTAATCCCTCGAGGGAATCACCCAGTTTTTTCAATGCGGGCATGCTCTTGTTTGAAACCATCGAGCAGCCTGCCAAAGAAAGTACACCCAGATTGGGCAGGACTCCACGGGCAAGAGCAGCAATTCCTGCATCGGTGATAGCGCACCTGGAAACATCAAGGTCGCTGAGTGCTGGACAGTTGCCTGCAATTTCCATCAAGCTAGAATCACCGACTCTTTTACAACCGTTGAGGTTTAACACTTTGATAGTCTTCCTGTGCAACTTGACCATGGACGATACTCCTGCGTCTGTCAGATTAATGCAACGGCTTAGATTAACATTAATCAAACTGGACTTGGACCTCTCAAGCAGAGAAAGAAACCCTAGGTCTGTTATTTCCTCAAGTCCAATCAATTCAAGACGCTGAAGCCGAAGACAGAGCTTTCCAAGGATGGCAAGAGTGGAGTTACCAACTCCAGGGCAGTTACGGATTGATAATGATGAAATTGATTCACATTGAGATACTGCGGTCAATTCCAGTTTCAGATCTTTGATCCCATGGCAGCTCACAAGAGTAAGAGCCTTCAATTTTGAACCACAGTTATAAAGGAGACCAAAAAGCCCAAAATGGGTCACCCTGTGGCACTCTTCCAAATGTAGGCTCACAACTGATGGGGCAGCCTTGGTAGAAACGATACCAGCCCATTCGTCCGACAAGAAAAAGCACACTTATGAAGATGTAAAGTTTTTCANATTTGGACCTAACCCCTTT
TARGET=18,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03304 Ps_contig_mira-and-tgicl-ass_franssen_29457
SEQUENCE=AAGCAAGTGGTATCAACGCAGAGTTGCTTATGAGGCAATTGCTGAAGCTGGATGGTATTAAGGCTGAAGGTGAAGCGAAGTTGCAGAGAAAAGCTGAGGTGCGTCGTGTGCAGAACGCCGTGGATACATTAGATTCCCTGAAGGCAAGAAACTCCAAACCTTTTAGCAACATTGGTAAGTCAGTCTTGTAACAAGACCTTTTGAAGCTTGAATGCTTCAACCTTGTCATCTTCTCCAAATGTAATTCAAAATTGGGAGTGGTTTGATTAGCTCAGAAATTATTGTGTGACATTAAATTACAAAGCACTTACAATATATATTTTTGGACCTTGATTCTTCATTTAAGGTCCAATTTTAAACATTTTTTCCGACTTTATAAAGAATAGTGAAAGGAAAATGGAGATTCATATTCTAGATATATACAATGCAATTAAGAAATAAGTAAACTTGTACTANAAGTATGATTATGTATTTGTTTGTTTGTAGCATGATACTAATGGATAGTAATAAAGTAGAAAATAAAGT
TARGET=472,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03305 Ps_contig_mira-and-tgicl-ass_franssen_29467
SEQUENCE=CGGGGATTTTCATTATTACGAATCTATCCATGGCTTCTTCTTTCACTTCGTTCTTCGATTCAACAAACCGATGGAATTTTGATACTCTCAAGAACTTCGGTCAAATTTCTCCCGTTGTGAGGAATCATCTCAAACAGGTTTACTTGACCTTGTGTTTTGCGGTTGCTGCTGCTGCTGTTGGAGCTTACCTTCATGTCCTCTTTCACCTTGGCGGGATTCTCACCACGATTGCGTGTGTGGGAACCAGTGTTTGGTTACTTTCAACTCCTCCTTATGAAGAGAGAAAGAGATTGAGTTTGTTGATGGCCGCGGCAATGTTTCAGGGTGCCTCTATTGGACCCTTGATTGATTTGGCTATTCAAGTTGATCCAAGCCTCATCTTCAGTTCATTTGTGGCAACTGCCTTGGCTTTTGGGTGTTTCTCTGGAGCAGCTTTGGTTGCTAAGCGTAGGGAGTACCTATATCTTGGTGGCTTGGTTTCTTCTGGGTTATCCATTCTTCTCTGGTTGCACTTTGCATCTTCCATCTTTGGAGGTTCAATGGCTATCTTTAAATTTGAGTTGTATTTTGGGCTTCTGGTGTTTGTGGGTTACATTGTAGTAGACACACAAGAAATAGTTGAGAAGGCACACTTTGGTGATCTGGATTATGTGAAGCATGCTTTGACCTTGTTTACTGATTTGGCTGCAATCTTTGTCCGGATTCTAGTTATCATGTTGAAGAATTCAGGAGAGAGGACTGAGAAAAAGAAGAAGAGAAGAGATTAATTTTCTTACCAAGCAAGCTTGTTTGGCGCTTCCCCTTTAATCTTTGCAAAATAGGAAGATACTAATAACAGTTGTCTGTAAAGAAGCGTTCTTCTGTCTTGAACTTTTTTCTACTGTGCAGTTAGTCCTGTTTCTGCTTTATAATTTATACTCCCTCATATCACTGTCATGGCAAAGATTCAAGTTCATTCTATGTTCGACA
TARGET=164,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2459
PRIMER_LEFT_PENALTY=0.241544
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=TCCATGGCTTCTTCTTTCACTT
PRIMER_RIGHT_SEQUENCE=GGACATGAAGGTAAGCTCCAAC
PRIMER_LEFT=26,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=60.242
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.2459
PRIMER_LEFT_1_PENALTY=0.241544
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=CCATGGCTTCTTCTTTCACTTC
PRIMER_RIGHT_1_SEQUENCE=GGACATGAAGGTAAGCTCCAAC
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=60.242
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=171
PRIMER_PAIR_PENALTY_2=0.2880
PRIMER_LEFT_2_PENALTY=0.283676
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=CACTTCGTTCTTCGATTCAACA
PRIMER_RIGHT_2_SEQUENCE=GGACATGAAGGTAAGCTCCAAC
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=60.284
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.3019
PRIMER_LEFT_3_PENALTY=0.199618
PRIMER_RIGHT_3_PENALTY=0.102276
PRIMER_LEFT_3_SEQUENCE=TTACTTGACCTTGTGTTTTGCG
PRIMER_RIGHT_3_SEQUENCE=GCTTGGATCAACTTGAATAGCC
PRIMER_LEFT_3=139,22
PRIMER_RIGHT_3=373,22
PRIMER_LEFT_3_TM=60.200
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.5000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.3019
PRIMER_LEFT_4_PENALTY=0.199618
PRIMER_RIGHT_4_PENALTY=0.102276
PRIMER_LEFT_4_SEQUENCE=TTACTTGACCTTGTGTTTTGCG
PRIMER_RIGHT_4_SEQUENCE=GGCTTGGATCAACTTGAATAGC
PRIMER_LEFT_4=139,22
PRIMER_RIGHT_4=374,22
PRIMER_LEFT_4_TM=60.200
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.5000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_03306 Ps_contig_mira-and-tgicl-ass_franssen_29476
SEQUENCE=GTATCACGGTCAGTACACTTGGGAGGCTTATCTGGGTAAGGTTTGGGAGATGGGGCAGACAAAACTGACCCATTCTCAGACAACACATACATATCCTCTGGTTCCATTCTTTCCTTCTGAACAGCAGAAGGAGACATGAGAATGAGTTGTTGAGGTTTGGGAATGGAATCATCATGGACTTTGATGCTGATGCTGCCACCAGTTCCTGAAACCCATCCCAAACCGTAGAAGTGGCGACACAGTTCCGCCATCAGAGCTTTCGTCTCCCTGACTGTCTTCCCATCCAGATACGCCTGAGACGACGTCGTCCCCACCTTTACTCCGTTTAAACCAATCGCCGCTGCCATTTCTAGAGAGAGAGAGAGTTCTTTGTGTGTGTGCTCCTGTGTTGGAATCAATCTGGCTTCGTCTCTTATGAGACACTTGAGAAC
TARGET=352,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4321
PRIMER_LEFT_PENALTY=0.024206
PRIMER_RIGHT_PENALTY=0.407941
PRIMER_LEFT_SEQUENCE=GGAATGGAATCATCATGGACTT
PRIMER_RIGHT_SEQUENCE=CAGATTGATTCCAACACAGGAG
PRIMER_LEFT=159,22
PRIMER_RIGHT=401,22
PRIMER_LEFT_TM=60.024
PRIMER_RIGHT_TM=59.592
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.4459
PRIMER_LEFT_1_PENALTY=0.037936
PRIMER_RIGHT_1_PENALTY=0.407941
PRIMER_LEFT_1_SEQUENCE=ATCATGGACTTTGATGCTGATG
PRIMER_RIGHT_1_SEQUENCE=CAGATTGATTCCAACACAGGAG
PRIMER_LEFT_1=170,22
PRIMER_RIGHT_1=401,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=59.592
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.4459
PRIMER_LEFT_2_PENALTY=0.037936
PRIMER_RIGHT_2_PENALTY=0.407941
PRIMER_LEFT_2_SEQUENCE=CATCATGGACTTTGATGCTGAT
PRIMER_RIGHT_2_SEQUENCE=CAGATTGATTCCAACACAGGAG
PRIMER_LEFT_2=169,22
PRIMER_RIGHT_2=401,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=59.592
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.5291
PRIMER_LEFT_3_PENALTY=0.024206
PRIMER_RIGHT_3_PENALTY=0.504940
PRIMER_LEFT_3_SEQUENCE=GGAATGGAATCATCATGGACTT
PRIMER_RIGHT_3_SEQUENCE=AGATTGATTCCAACACAGGAGC
PRIMER_LEFT_3=159,22
PRIMER_RIGHT_3=400,22
PRIMER_LEFT_3_TM=60.024
PRIMER_RIGHT_3_TM=60.505
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.5429
PRIMER_LEFT_4_PENALTY=0.037936
PRIMER_RIGHT_4_PENALTY=0.504940
PRIMER_LEFT_4_SEQUENCE=ATCATGGACTTTGATGCTGATG
PRIMER_RIGHT_4_SEQUENCE=AGATTGATTCCAACACAGGAGC
PRIMER_LEFT_4=170,22
PRIMER_RIGHT_4=400,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=60.505
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_03307 Ps_contig_mira-and-tgicl-ass_franssen_29476
SEQUENCE=GTATCACGGTCAGTACACTTGGGAGGCTTATCTGGGTAAGGTTTGGGAGATGGGGCAGACAAAACTGACCCATTCTCAGACAACACATACATATCCTCTGGTTCCATTCTTTCCTTCTGAACAGCAGAAGGAGACATGAGAATGAGTTGTTGAGGTTTGGGAATGGAATCATCATGGACTTTGATGCTGATGCTGCCACCAGTTCCTGAAACCCATCCCAAACCGTAGAAGTGGCGACACAGTTCCGCCATCAGAGCTTTCGTCTCCCTGACTGTCTTCCCATCCAGATACGCCTGAGACGACGTCGTCCCCACCTTTACTCCGTTTAAACCAATCGCCGCTGCCATTTCTAGAGAGAGAGAGAGTTCTTTGTGTGTGTGCTCCTGTGTTGGAATCAATCTGGCTTCGTCTCTTATGAGACACTTGAGAAC
TARGET=371,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6629
PRIMER_LEFT_PENALTY=0.037936
PRIMER_RIGHT_PENALTY=0.624930
PRIMER_LEFT_SEQUENCE=ATCATGGACTTTGATGCTGATG
PRIMER_RIGHT_SEQUENCE=AAGAGACGAAGCCAGATTGATT
PRIMER_LEFT=170,22
PRIMER_RIGHT=413,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=59.375
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.6629
PRIMER_LEFT_1_PENALTY=0.037936
PRIMER_RIGHT_1_PENALTY=0.624930
PRIMER_LEFT_1_SEQUENCE=CATCATGGACTTTGATGCTGAT
PRIMER_RIGHT_1_SEQUENCE=AAGAGACGAAGCCAGATTGATT
PRIMER_LEFT_1=169,22
PRIMER_RIGHT_1=413,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=59.375
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.7088
PRIMER_LEFT_2_PENALTY=0.083883
PRIMER_RIGHT_2_PENALTY=0.624930
PRIMER_LEFT_2_SEQUENCE=CTTTCGTCTCCCTGACTGTCTT
PRIMER_RIGHT_2_SEQUENCE=AAGAGACGAAGCCAGATTGATT
PRIMER_LEFT_2=256,22
PRIMER_RIGHT_2=413,22
PRIMER_LEFT_2_TM=59.916
PRIMER_RIGHT_2_TM=59.375
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=158
PRIMER_PAIR_PENALTY_3=0.7958
PRIMER_LEFT_3_PENALTY=0.170871
PRIMER_RIGHT_3_PENALTY=0.624930
PRIMER_LEFT_3_SEQUENCE=TTCTGAACAGCAGAAGGAGACA
PRIMER_RIGHT_3_SEQUENCE=AAGAGACGAAGCCAGATTGATT
PRIMER_LEFT_3=114,22
PRIMER_RIGHT_3=413,22
PRIMER_LEFT_3_TM=60.171
PRIMER_RIGHT_3_TM=59.375
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.9036
PRIMER_LEFT_4_PENALTY=0.037936
PRIMER_RIGHT_4_PENALTY=0.865622
PRIMER_LEFT_4_SEQUENCE=ATCATGGACTTTGATGCTGATG
PRIMER_RIGHT_4_SEQUENCE=GAAGCCAGATTGATTCCAACAC
PRIMER_LEFT_4=170,22
PRIMER_RIGHT_4=406,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=60.866
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_03308 Ps_contig_mira-and-tgicl-ass_franssen_29478
SEQUENCE=TCAAAACGAAACGAAACGAAACCCTTCTAATATAAAAACCCTAATTTGTTTATYCCCTACTCTTCTTTCCTCCTCCATCCGCCTCCCCTCTCAAGCATCTCCATCCTCTTCCTCCCGTTTCACCACCATCCGATGCCGAATCAACCGTACCGGAAATTCGACCTTCTCCCCACTGGACTCAAGAAATTCGAATTTCAGCGAGCGGCCACCCATGGACATGGCCCCACTCTTCCCTGGTTGTGACTATGAACACTGGCTTATTGTTATGGATAAGCCTGGCGGTGAAGGTGCCACTAAACAGAAAATGATTGATTGCTACGTTCAAACCCTCGCTAAGGTCCTTGGAAGTGAAGAGGAAGCTAAGAAGAAGATTTACAATGTTTCCTGTGAGAGGTACTTTGGATTTGGATGTGAGATTGATGAGGAGACATCTAATAAGCTGGAAGGGTTGCCTGGAGTTCTGTTTGTTCTCCCAGATTCATATGTTGATCCTGAAAACAAGGACTATGGTGCTGAGTTATTTGTGAATGGAGAGATCGTACAGAGGTCACCTGAAAGACAGAAAAGGGTGGAGCCACAGCCACAGAGACATCAAGACAGACCGAGATACAATGATAGAACAAGGTATGTCCGACGCAAAGAAAGTACAAGATGAAGTAGATTTCAGTAACAAGGGGACACGGCAATATAGAGGGCAAGAGAGAGACATGCTATGAAAGAATGAAGGCTGAACTACTATACTCCAGCCTTTGCATGCAGAAATCGTATTTGTTGGATGTTCTATTGGTAAATAGATATGTTTGTAGTTTGAACCAATATGAATATAAACTTTATTATGCCATCAAATGTCTTGTCCTTAATCAATTCTCTAACAAAATGTTATAAACTATTGTTGTTTTTATTGGTATACTATTATTTATTGTAATCAGATTCGGTTTGATAGAAATCATACTTACGTAGAGTATAACTCTCATTAGATTA
TARGET=4,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03309 Ps_contig_mira-and-tgicl-ass_franssen_29482
SEQUENCE=TTAGCGTTTTCTCTCCNATACCCTTCGCTTCCAACCCTTCTTATCCCCTCTCCCTCGCTCCCGAAACCTCACAACAGCACGTTCCGAACTCGTTGCTCTCACTCGCTCCGATTCCGCAACTCGCTTCGTTCCATTTCTCGTTGATCGTCATGCCGAAAGCCAAATCCGACTCCAAAGCCGTTGATAGCAGGTTGAAACGCAAAGGCGCTGGAACTGGAAGGAAGCAGTCGAAGAAAGCTGCTAAGGATCCAAACAAGCCTAAGAGGCCTCCCAGTGCTTTCTTCGTTTTCATGGCTGATTTCAGAGAACAGTATAAGAAGGATCATCCTAACAACAAATCTGTTGCTGCTGTTGGAAAAGCTTGTGGTGAGGAATGGAAATCTTTGTCTGAAGAGGATAAGGCTCCTTATGTTGCTAGGGCATTGAAGAAGAAAGAAGAGTATGAGATAACTCTTCAGGCCTACAATAAAAAACTGGAGGGTAAAGATGATGAAGAAGGTTCTGACAAGTCAAAATCTGAAGTCAATGATGAGGATGAAGATGAGGAGGATGAGGAGGATGATGATGATGAGTAGAGTTGCTGTTATACAACAAAATGAAGAAGGTTCTGACAAGCTTAGGAAAGATGGGGAAACATATGTTATGTTTAGATTGGTTTATGATATGTTGGGAAGTTTCACTAATGCACTACTGTTATTTTCAAGATAGTTTATGGATTTTTATTGTCTTTTCTAGAGGTTTAGATAGTGAACAATTCTAATTAACTTTCAATATCATTATCCTGTACTTAAAGTCAATGCTATGATACTTTCTCAATTCTGGGTCTTNACAATTAAGTT
TARGET=558,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6404
PRIMER_LEFT_PENALTY=0.036956
PRIMER_RIGHT_PENALTY=0.603423
PRIMER_LEFT_SEQUENCE=GATGAGGATGAAGATGAGGAGG
PRIMER_RIGHT_SEQUENCE=CCCATCTTTCCTAAGCTTGTCA
PRIMER_LEFT=527,22
PRIMER_RIGHT=629,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.603
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=103
PRIMER_PAIR_PENALTY_1=0.6404
PRIMER_LEFT_1_PENALTY=0.036956
PRIMER_RIGHT_1_PENALTY=0.603423
PRIMER_LEFT_1_SEQUENCE=ATGAGGATGAAGATGAGGAGGA
PRIMER_RIGHT_1_SEQUENCE=CCCATCTTTCCTAAGCTTGTCA
PRIMER_LEFT_1=528,22
PRIMER_RIGHT_1=629,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=60.603
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=0.7550
PRIMER_LEFT_2_PENALTY=0.151533
PRIMER_RIGHT_2_PENALTY=0.603423
PRIMER_LEFT_2_SEQUENCE=TGTCTGAAGAGGATAAGGCTCC
PRIMER_RIGHT_2_SEQUENCE=CCCATCTTTCCTAAGCTTGTCA
PRIMER_LEFT_2=384,22
PRIMER_RIGHT_2=629,22
PRIMER_LEFT_2_TM=59.848
PRIMER_RIGHT_2_TM=60.603
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=0.7681
PRIMER_LEFT_3_PENALTY=0.164639
PRIMER_RIGHT_3_PENALTY=0.603423
PRIMER_LEFT_3_SEQUENCE=GGGCATTGAAGAAGAAAGAAGA
PRIMER_RIGHT_3_SEQUENCE=CCCATCTTTCCTAAGCTTGTCA
PRIMER_LEFT_3=417,22
PRIMER_RIGHT_3=629,22
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=60.603
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.8613
PRIMER_LEFT_4_PENALTY=0.257927
PRIMER_RIGHT_4_PENALTY=0.603423
PRIMER_LEFT_4_SEQUENCE=TATGTTGCTAGGGCATTGAAGA
PRIMER_RIGHT_4_SEQUENCE=CCCATCTTTCCTAAGCTTGTCA
PRIMER_LEFT_4=407,22
PRIMER_RIGHT_4=629,22
PRIMER_LEFT_4_TM=59.742
PRIMER_RIGHT_4_TM=60.603
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_03310 Ps_contig_mira-and-tgicl-ass_franssen_29491
SEQUENCE=CTATGATCTTCCTACCCAACCTTTTGTAAANTTTATTTTCTTTCCATCGCATTGGGCGAAGTGGGCGTTTCGGAAGAAAAGGTGTTGCTATAAACTTTGTCACTGTGGATGATGCAAGAATGCTGTCTGATATTCAGAAGTTCTATAATGTCACCGTAGAGGAATTGCCTTCAAATGTCGCCGATCTTCTCTGATGAGTTAATTTTTGTTCTTTGTTAAGTCATCTAGTTTTTTTTTTTAGTTTTTGACAAATGCAATTTTCTGTTTTTTTTCTTTACCATTTTTACCCATTAGAGATCAAAGACATCTGACTCTATTTATAATGGATTTATGAATTTTGTTTTATTGCTTAATTAATTAAAAGGATGGTCTAGACTTTTTCATTTCTGCCGTATGTATTTTCTTTTTAATATGATTTTATTTTAAAAGTATTTATGTAATTTAAATTAATTGGTTTTCG
TARGET=350,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.9468
PRIMER_LEFT_PENALTY=0.042736
PRIMER_RIGHT_PENALTY=4.904113
PRIMER_LEFT_SEQUENCE=ACTTTGTCACTGTGGATGATGC
PRIMER_RIGHT_SEQUENCE=CGGCAGAAATGAAAAAGTCT
PRIMER_LEFT=93,22
PRIMER_RIGHT=391,20
PRIMER_LEFT_TM=60.043
PRIMER_RIGHT_TM=57.096
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=5.2740
PRIMER_LEFT_1_PENALTY=0.369874
PRIMER_RIGHT_1_PENALTY=4.904113
PRIMER_LEFT_1_SEQUENCE=CGTAGAGGAATTGCCTTCAAAT
PRIMER_RIGHT_1_SEQUENCE=CGGCAGAAATGAAAAAGTCT
PRIMER_LEFT_1=154,22
PRIMER_RIGHT_1=391,20
PRIMER_LEFT_1_TM=59.630
PRIMER_RIGHT_1_TM=57.096
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=5.3059
PRIMER_LEFT_2_PENALTY=0.401769
PRIMER_RIGHT_2_PENALTY=4.904113
PRIMER_LEFT_2_SEQUENCE=AGAGGAATTGCCTTCAAATGTC
PRIMER_RIGHT_2_SEQUENCE=CGGCAGAAATGAAAAAGTCT
PRIMER_LEFT_2=157,22
PRIMER_RIGHT_2=391,20
PRIMER_LEFT_2_TM=59.598
PRIMER_RIGHT_2_TM=57.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=235
PRIMER_PAIR_PENALTY_3=5.4300
PRIMER_LEFT_3_PENALTY=0.525918
PRIMER_RIGHT_3_PENALTY=4.904113
PRIMER_LEFT_3_SEQUENCE=TCACTGTGGATGATGCAAGAAT
PRIMER_RIGHT_3_SEQUENCE=CGGCAGAAATGAAAAAGTCT
PRIMER_LEFT_3=99,22
PRIMER_RIGHT_3=391,20
PRIMER_LEFT_3_TM=60.526
PRIMER_RIGHT_3_TM=57.096
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=5.7107
PRIMER_LEFT_4_PENALTY=0.806597
PRIMER_RIGHT_4_PENALTY=4.904113
PRIMER_LEFT_4_SEQUENCE=GATGATGCAAGAATGCTGTCTG
PRIMER_RIGHT_4_SEQUENCE=CGGCAGAAATGAAAAAGTCT
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=391,20
PRIMER_LEFT_4_TM=60.807
PRIMER_RIGHT_4_TM=57.096
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_03311 Ps_contig_mira-and-tgicl-ass_franssen_29493
SEQUENCE=TCTAGTCGAACAAAAAAGATGTAGGGATACACAATAATTGAATGAAATATTATGATTGTGGCTTAAGAATCTCAGTACATTCCACCGACTTGTAACAGTTATAGAAATTAAACTTCAAAACTATTTTACAAAGTTATCCGTGTCCCGTTCTCCCATAAAAAGTTTGCTCAATGCGTTGAATTGTTGAACCTGAAAAGCAGTTTCCTAGTTTCACAGACGCGTGCAAATATATCTTACTGTATTATTATACATAACATAGCCCAACACGTTTGACAATCCATCAAATGTCATGTTTCATAAGATAAGCTACAAACCTTATAAGGGAGAAGTTTAAAACCAAGATATTAGCTTTCTCACAAACATACTATAGATTGACTTCTCTGTCCTCTTATCCTTGGAGTGCAGAAACATCCAAGAAACAGATAACCGCTAATGATGCCTTGTGACTAAGTTTTTGCCATATGCTAACCTTTTTAACGAATTGGGTGATATTGTGTGATTCCTCGCTTAGGCGGTAGTTTTCTCAAAATGAAAAGGACCAAAGAAGAAGGCAATATTTCCACCAACAGGAAATATATAAGGTTTAGAATGGGATGGTCCAATACATCAATGTTAGCCGCTTTATCAAGTGCATTAAAGCACATCATAACACATCTGATAAGGAAACATGAAAAACATATGGTAGTCACGTAGCCGACCTCTTGTAGCTTCTTGCGGCGACCTTTGGATTCAACAGGGAAGCGTTGCAGCATCAAGAATAATCTCCCACCATAGAGTAGAAAGCCAAGAGCCGCAAACAAAGAGACCCCTGCAAAGAATATCTTTGACAAAATGACCAGCATGCTGATAGGTTTCCACCATAATATCAACCACAAAGCAATCTGAATAACATAAACCACAGCATTAATCGTGTAGAAACTTGGTCTCAGACCGTCGGTAGATACAGCTCGTGCCTGATAATAAATCTCAGCCCAGAAGAGAACCAAAAGTGCATATGTGGTAAAGAAAGCAAGACTGGGCATGTCAAGTAACATATGTTGGACAATCTCTGGCTGTAACTTCTGCACATCTCGACGGAAAACGAAAACTAAACATCTAACCCCATTGACGAAAAAATTGAGAAAGTGAAAGACCTTCTGCGTCGTCCAACCATATTCCGGAACTCTCAATTGTATTCGAACAAGTTGAACGAGAGCGATGGCGGCTACGATTCCGTAGAGGACGGCAAGGAGATGGAAAGTACGATCCTGCCAGAGAGGGGAGTCGTTGATATCTCTCCACCAGTTGGATGTCTCGGTGAAGTCAAGGGAGGCGACCACGGCCATAACCGCTTCCCCCGGCACGCCTCTGGCCACCATCGAATCGGTGAATGATGATTTAATACAGCCTTTAATATATATATTTTTTCTTCTACAATGAAATAAACACAGAAAGAAAAGGAAGAGAAGAAGAAACAAAAGGAATCTAATCTATTCTAATCTAATTATTCAATCAATGTTGATTTTTTTGGGGTTTTGTTTTGTGGCCGGCGATATAGAGAAATAATTTAGGATTTAGAGAGAGGAAACGGACGGTAACGTCGGTAAGGATGGAAAGTCCCNNYNAGT
TARGET=1393,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03312 Ps_contig_mira-and-tgicl-ass_franssen_29495
SEQUENCE=GGTATCATCGGGGAATTCAAAACACAAAAAAAATTCCCTTTTTATTTTCCTCTTCTTCTCCTCCTTCTTCTTCTTCTCCTCTCTCCGTTTGGTGCCATTCCATATCCACGTGGAGCGCCGATCCGATCACCCTCCCTCCGTAATTCACCCTCTCCGGCCATTTCTCCTTCCCCGTCATGAATCCCGAGTATGATTATCTGTTCAAGCTCCTTCTTATTGGAGACTCTGGTGTTGGTAAATCGTGCCTTCTTCTAAGATTTGCTGATGATTCATACATTGAAAGCTACATAAGCACTATAGGAGTTGATTTTAAAATACGGACTGTTGAGCAGGATGGGAAGACAATTAAACTACAGATTTGGGATACCGCCGGACAAGAACGATTTAGGACAATAACCAGTAGCTACTATCGTGGGGCACATGGAATCATTATTGTTTATGATGTGACAGATGAAGAGAGCTTCAATAATGTGAAGCAGTGGCTCAGTGAAATTGACCGCTATGCCAGTGATAATGTTAACAAGCTTTTGGTTGGAAACAAGAGTGATCTGACAGACAAATAGAGTTGTATCATATGATACAGACCAAAGTATTTGCAGATACAATCGGCTACTTACCTTTAGAGGAAAC
TARGET=64,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4522
PRIMER_LEFT_PENALTY=0.444960
PRIMER_RIGHT_PENALTY=0.007205
PRIMER_LEFT_SEQUENCE=GTATCATCGGGGAATTCAAAAC
PRIMER_RIGHT_SEQUENCE=GAAGAAGGCACGATTTACCAAC
PRIMER_LEFT=1,22
PRIMER_RIGHT=251,22
PRIMER_LEFT_TM=59.555
PRIMER_RIGHT_TM=60.007
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.5741
PRIMER_LEFT_1_PENALTY=0.444960
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=GTATCATCGGGGAATTCAAAAC
PRIMER_RIGHT_1_SEQUENCE=AGAAGAAGGCACGATTTACCAA
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=252,22
PRIMER_LEFT_1_TM=59.555
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.8118
PRIMER_LEFT_2_PENALTY=0.444960
PRIMER_RIGHT_2_PENALTY=0.366796
PRIMER_LEFT_2_SEQUENCE=GTATCATCGGGGAATTCAAAAC
PRIMER_RIGHT_2_SEQUENCE=TTACCAACACCAGAGTCTCCAA
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=237,22
PRIMER_LEFT_2_TM=59.555
PRIMER_RIGHT_2_TM=59.633
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=0.8118
PRIMER_LEFT_3_PENALTY=0.444960
PRIMER_RIGHT_3_PENALTY=0.366796
PRIMER_LEFT_3_SEQUENCE=GTATCATCGGGGAATTCAAAAC
PRIMER_RIGHT_3_SEQUENCE=TTTACCAACACCAGAGTCTCCA
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=59.555
PRIMER_RIGHT_3_TM=59.633
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.8279
PRIMER_LEFT_4_PENALTY=0.444960
PRIMER_RIGHT_4_PENALTY=0.382951
PRIMER_LEFT_4_SEQUENCE=GTATCATCGGGGAATTCAAAAC
PRIMER_RIGHT_4_SEQUENCE=GCAAATCTTAGAAGAAGGCACG
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=261,22
PRIMER_LEFT_4_TM=59.555
PRIMER_RIGHT_4_TM=60.383
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=261
=
PRIMER_SEQUENCE_ID=SSR_03313 Ps_contig_mira-and-tgicl-ass_franssen_29497
SEQUENCE=GCGGAAACACAAACTTGATATAAAAAGAAACAAATGATTAGATACACATATATTCTCAACAGTGACTCCGTTTCAATTACAGCATATATTGACAACCTTGGAATCAGATCTCATCATACAAATTAACCCAAACAAGTAGCCAATTTGAACAAGAAAACGTTTCCAAAAACAACACCCAAGGTTAGCTACATAGCATAAAAGCGTTTCAGGCAAAAACAAAAAGCAAAAACAAGCACAACTAGGTTAATTGATTGATCCACTTGAAGAAACCGAATGAAAATCACAAATTAAACATAGAAAATACCGAAACACTAGAAAAAGCGATTTGAATAGAAGGATCTGGCCTCACTTAGCAACCATTCTAGCGATGAAATCCTCGTAGCGAATCTTGCCGTCAGAACCAACATCAACTTCCCTGATCCATTCATCAAACTCAGCAGGCTCAAGCTTCTCACCGATACTGGTGAGAATGTGACGAAGCTCGTTGACGGAGACGAATCCGGTGGAATCCTTGTCGAGAACCTTGAAAGCATCGCGTAACTGACGATCGAACGGCTCAGGTTTCATGTGTTTGGACATGAGATCCAGAAACCGAGCGAAATCGAACGGTGATGTGAGATTCTCTTCCGCAACGATGGATTTGAGTTGAGCTTGGGTCGGGTTTCCGCCGAGAGATCGCATGAGGATTCCGAGCTCCGATGGAGCGATCTTGCCGTCGCCGTCGGTGTCGAATAGAGTGAAAGCTTCTTTCATGGAAGAAATTTGATCGTCGCTCAGATCCTTGCCCATTTCGGTTTCGTTTCGATTTTCTGGTTTTATTATTACAAGAAGAAGAAGATGAAGAAATTGAAAGTGTTTTCCCACTCTCTCTTTTTCAGTTTCTGTTCTCCCCCCGATGATTACGGTGGAATGTGAGTTTTATCTACGACGTCGTNATCCCCGCGTTACTTTCTGCGTTGATACCACT
TARGET=826,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5277
PRIMER_LEFT_PENALTY=0.137489
PRIMER_RIGHT_PENALTY=0.390220
PRIMER_LEFT_SEQUENCE=ACGATGGATTTGAGTTGAGCTT
PRIMER_RIGHT_SEQUENCE=GGGGAGAACAGAAACTGAAAAA
PRIMER_LEFT=631,22
PRIMER_RIGHT=891,22
PRIMER_LEFT_TM=60.137
PRIMER_RIGHT_TM=59.610
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.8212
PRIMER_LEFT_1_PENALTY=0.137489
PRIMER_RIGHT_1_PENALTY=0.683672
PRIMER_LEFT_1_SEQUENCE=ACGATGGATTTGAGTTGAGCTT
PRIMER_RIGHT_1_SEQUENCE=CTCACATTCCACCGTAATCATC
PRIMER_LEFT_1=631,22
PRIMER_RIGHT_1=915,22
PRIMER_LEFT_1_TM=60.137
PRIMER_RIGHT_1_TM=59.316
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=1.0121
PRIMER_LEFT_2_PENALTY=0.621889
PRIMER_RIGHT_2_PENALTY=0.390220
PRIMER_LEFT_2_SEQUENCE=TCGGTGTCGAATAGAGTGAAAG
PRIMER_RIGHT_2_SEQUENCE=GGGGAGAACAGAAACTGAAAAA
PRIMER_LEFT_2=721,22
PRIMER_RIGHT_2=891,22
PRIMER_LEFT_2_TM=59.378
PRIMER_RIGHT_2_TM=59.610
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=1.0294
PRIMER_LEFT_3_PENALTY=0.639172
PRIMER_RIGHT_3_PENALTY=0.390220
PRIMER_LEFT_3_SEQUENCE=ATGTGAGATTCTCTTCCGCAAC
PRIMER_RIGHT_3_SEQUENCE=GGGGAGAACAGAAACTGAAAAA
PRIMER_LEFT_3=611,22
PRIMER_RIGHT_3=891,22
PRIMER_LEFT_3_TM=60.639
PRIMER_RIGHT_3_TM=59.610
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=1.1428
PRIMER_LEFT_4_PENALTY=0.752572
PRIMER_RIGHT_4_PENALTY=0.390220
PRIMER_LEFT_4_SEQUENCE=AAATCGAACGGTGATGTGAGAT
PRIMER_RIGHT_4_SEQUENCE=GGGGAGAACAGAAACTGAAAAA
PRIMER_LEFT_4=598,22
PRIMER_RIGHT_4=891,22
PRIMER_LEFT_4_TM=60.753
PRIMER_RIGHT_4_TM=59.610
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_03314 Ps_contig_mira-and-tgicl-ass_franssen_29498
SEQUENCE=TAATTTACTAAAATAATGCAGTTTTAAATTTTGGGTTGGCCGNGGCAACAAGACCCAAATTTCAAAAGTAAAACAACATTTCACAACCCAACTTAAAAAATTAACTTCGTCAGTAAAATAACATACATAACTAAGCATGAAANCATTGCCCAAGAAACCAGTTAAGAGATGGTGAAAGTTAATCATCATCTTCGAGAACACTTCGACGCCTCTGTATTGTAACTTTCGTTTGCTTTGCTGTTTGGCTGTTAATCTGCTCCAACAGAGTTATGAGCCTTTCTTCAGTAACCTTTTCAGCTATCTGACCCATTTGAGCAGCTCTCAATATAACATCTTCCACACCTTTTGCTTTCTCTGGTTCACAAGCAATTCGAGCAAGTTCTTTCTCGTGCCTCAGCCGACAGTATCTGAACTAAGCATCTATTTGTTCCTTTCCTTTCCTCTCGCCCTTCCCTT
TARGET=428,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03315 Ps_contig_mira-and-tgicl-ass_franssen_29499
SEQUENCE=TATAACAGAATCNCTNGATTGAAATACAGTTTTTGGTTCGTAAAAAAGCGCTTATTCCAAACGCGATCTGTGGTGGTGGTGTTGCTGCAGAAACATCAAGGCAAAACCAAAACCCCCATTCACACACCCGCTAGGGTATCTTTCTTCTGCTAACCAAACCAAATGGCTGATCCTGAGCTGGAAGCTATCAGACAGAGAAGGATGCAGGAGCTTATGGGTCGGCATGGCACGGGAAATCAACCGAACCCTGAACAGCAGAGCGCTCAGGAGGATGCAAAGAGGGAGGCAGAGGAAAGAAGACAAATGATGCTTAGTCAGATACTGTCGGCTGAGGCACGAGAAAGACTTGCTCGAATTGCTTTGGTGAAACCAGAGAAAGCAAAAGGTGTGGAAGATGTTATATTGAGAGCTGCTCAAATGGGTCAGATAGCTGAAAAGGTTACTGAAGAAAGGCTCATAACTCTGTTGGAGCAGATTAACAGCCAAACAGCAAAGCAAACGAAAGTTACAATACAGAGGCGTCGAAGTGTTCTCGAAGATGATGATTAGCTTTCACCATCTCTTAACTGGTTTCTTGGGCAATGATTTCATGCTTAGTTATGTATGTATTTTACTGACGAAGTATTTTTTAAGTGGGTTGTGAAATGTGTTTTACTTTGAAATTTTGGGCTTGTTGCCTGGCCACCAAATTAAACTGCATATTTGAAATATTTGTGCAAATGAATGGAAGTTAAAGCATGTTTTGTGAGGAAGGGAGAATGCATAAAAATGAAATTGTAATTTCTTAAGCTTTCAATTTGAATTAAAAATTATTTTTTATAYAAA
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03316 Ps_contig_mira-and-tgicl-ass_franssen_29501
SEQUENCE=CGTTCGGGGGAAAAGAAAAAGGCAAATAGAAAACGACATGCGTTGAATCATAACGATTCATAAATGAACGACAAGAGGAAAAACAAGAGAGTGGTTAATTTGAATTGAATTGAATAGATCCACCGTTAATTCATCTCTCTCTTCGTAACCTGAGTCGAAAATCAACGGATCAAAATTCAGAATGGGAATTCTCTACGCACTGGTGGCGCGTGGAACGGTGGTTCTGGCAGAGTTCACCGGAACCACCACAAACGCAAGCGCAATCGCGAGACAGATTCTCGAGAAGATTCCCGGAGACAACGATACTCATATATCGTATTCTCAAGATCGTTACATCTTTCACGTTAAACGGACCGACGGACTTACCGTTCTTTGTATGGCGGATGAGAACGCCGGAAGGAGAATTCCGTTTGCGTTTCTTGAGGAGATTCATCAGAAGTTTGTTAGGACTTATGGACGCGCGGTTCTTAGTGCGCAAGCTTATGGAATGAATGATGAGTTTTCTAGGGTTTTGAGTCAGCAAATGGAGTATTTCTCTAGTGATCCTAATGCGGATAGGATTAATCGGCTCAAAGGTGAAATGTCTCAGGTACGAAATGTCATGATAGAAAATATTGACAAGGTTTTGGATAGAGGTGATAGATTGGAATTGCTGGTGGATAAAGCAGCTAATATGCAAGGAAACACATTTCGCTTCAGGAAGCAAGCTCGCCGTTTTAGAAGCAATGTGTGGTGGAGGAATGTCAAACTCACGATTGCTCTTATACTCGTCCTACTTTTGATAGCCTATGGTGTGTTGGCTTTTGTTTGCCATGGGCCTGCGCTACCGTCTTGCTTCTAGGGCTGGAATGCGAGTGCTATGTGTGCTTCGACATCGTCTTTGGGTTGCAGATGTCACTTCTATTAATTTTTAGTTGCTGGGCTGTCAACATGTGTGCTAGCAAAAGCCTTGCTGCATATTCCCTGGTGTTTTGGGTTGTATTTTTCATTACAGAGGTTTGAGGAAAAAATATTGCAAATCATGTCTTTTATGTATTTTCATATCACCCAAAAAATTGGAGTGAACTGGAACATGATTGGGTTATTATAAAGTGGTGTTCCCTTCTCTTTTTGTTCAAGAGACTAAAGGGTAAACTTTTGAATTCGTGTATCCCTTTATTGCTCAATGAAGGGCAAATTTTTAAAAATTTTCATTCGCCCCTATTTGTTATAATAAAT
TARGET=100,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6071
PRIMER_LEFT_PENALTY=0.104555
PRIMER_RIGHT_PENALTY=0.502561
PRIMER_LEFT_SEQUENCE=ACGACATGCGTTGAATCATAAC
PRIMER_RIGHT_SEQUENCE=CAGTGCGTAGAGAATTCCCATT
PRIMER_LEFT=32,22
PRIMER_RIGHT=201,22
PRIMER_LEFT_TM=59.895
PRIMER_RIGHT_TM=60.503
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.6864
PRIMER_LEFT_1_PENALTY=0.104555
PRIMER_RIGHT_1_PENALTY=0.581808
PRIMER_LEFT_1_SEQUENCE=ACGACATGCGTTGAATCATAAC
PRIMER_RIGHT_1_SEQUENCE=GCGTAGAGAATTCCCATTCTGA
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=59.895
PRIMER_RIGHT_1_TM=60.582
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=166
PRIMER_PAIR_PENALTY_2=0.7510
PRIMER_LEFT_2_PENALTY=0.497481
PRIMER_RIGHT_2_PENALTY=0.253482
PRIMER_LEFT_2_SEQUENCE=AGGCAAATAGAAAACGACATGC
PRIMER_RIGHT_2_SEQUENCE=TGATTTTCGACTCAGGTTACGA
PRIMER_LEFT_2=19,22
PRIMER_RIGHT_2=163,22
PRIMER_LEFT_2_TM=60.497
PRIMER_RIGHT_2_TM=59.747
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=0.9830
PRIMER_LEFT_3_PENALTY=0.104555
PRIMER_RIGHT_3_PENALTY=0.878426
PRIMER_LEFT_3_SEQUENCE=ACGACATGCGTTGAATCATAAC
PRIMER_RIGHT_3_SEQUENCE=TTCGACTCAGGTTACGAAGAGA
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=158,22
PRIMER_LEFT_3_TM=59.895
PRIMER_RIGHT_3_TM=59.122
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=1.0000
PRIMER_LEFT_4_PENALTY=0.497481
PRIMER_RIGHT_4_PENALTY=0.502561
PRIMER_LEFT_4_SEQUENCE=AGGCAAATAGAAAACGACATGC
PRIMER_RIGHT_4_SEQUENCE=CAGTGCGTAGAGAATTCCCATT
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=201,22
PRIMER_LEFT_4_TM=60.497
PRIMER_RIGHT_4_TM=60.503
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_03317 Ps_contig_mira-and-tgicl-ass_franssen_29505
SEQUENCE=TTTGGGTTCTTTGGAATGGAGTGATGGAGTTCATATTGTGAGGAGCCCAATTGCTGCTAGATGGAGCAATGGATTCTCATCAGCATTCCTTTTGTATTTTCATTCTGTAGAAAGTGGAAACACTATTTATTATTATTACCATGCATCTGAGACCACGACAACGGAGG
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3412
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=2.309402
PRIMER_LEFT_SEQUENCE=GAGTTCATATTGTGAGGAGCCC
PRIMER_RIGHT_SEQUENCE=CGTTGTCGTGGTCTCAGATG
PRIMER_LEFT=26,22
PRIMER_RIGHT=162,20
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.309
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=2.3412
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=2.309402
PRIMER_LEFT_1_SEQUENCE=GGAGTTCATATTGTGAGGAGCC
PRIMER_RIGHT_1_SEQUENCE=CGTTGTCGTGGTCTCAGATG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=162,20
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=60.309
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=2.6708
PRIMER_LEFT_2_PENALTY=0.361394
PRIMER_RIGHT_2_PENALTY=2.309402
PRIMER_LEFT_2_SEQUENCE=CTTTGGAATGGAGTGATGGAGT
PRIMER_RIGHT_2_SEQUENCE=CGTTGTCGTGGTCTCAGATG
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=162,20
PRIMER_LEFT_2_TM=60.361
PRIMER_RIGHT_2_TM=60.309
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=2.7659
PRIMER_LEFT_3_PENALTY=0.031769
PRIMER_RIGHT_3_PENALTY=2.734128
PRIMER_LEFT_3_SEQUENCE=GAGTTCATATTGTGAGGAGCCC
PRIMER_RIGHT_3_SEQUENCE=GTTGTCGTGGTCTCAGATGC
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=161,20
PRIMER_LEFT_3_TM=59.968
PRIMER_RIGHT_3_TM=59.266
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=2.7659
PRIMER_LEFT_4_PENALTY=0.031769
PRIMER_RIGHT_4_PENALTY=2.734128
PRIMER_LEFT_4_SEQUENCE=GGAGTTCATATTGTGAGGAGCC
PRIMER_RIGHT_4_SEQUENCE=GTTGTCGTGGTCTCAGATGC
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=161,20
PRIMER_LEFT_4_TM=59.968
PRIMER_RIGHT_4_TM=59.266
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_03318 Ps_contig_mira-and-tgicl-ass_franssen_29506
SEQUENCE=CCTCGGACTCGCCGAATCCTCCGGATTATGGTTCCTTAAACTTTCTTACTYACTTTACTTCGCCTCCGACGTCATCATCGGCGTCCTCGACACCGGAATCTGGCCGGAGCTCCAAAGCTTCTCCGATCAATCTCTATTTCCTTCCACTCTCCCTTCTTCCTGGAAAGGAGCCTGCGAAACATCCCATGATTTCCCTAAATCTTCATGTAACGGAAAAATCATCGGCGCGAAATCTTTCTACAAAGGCTACGAAGCTTACCTTCAAAGACCTATTGATGAAACCCTCGAATCGAAATCTCCTAGAGATACAGAAGGCCATGGAACTCACACAGCTTCAACTGCAGCAGGCTCCATCGTTGGTAACGCTAGTTTGTTTCATTTCGCTCGAGGTGAAGCNAAGGGAATGGCGACAAAAGCTAGAATCGCTGCTTACAAAATCTGTTGGAAATCTGGTTGTTTTGATTCTGATATTCTTGCTGCTATGGATGAAGCTGTCTCTGATGGGGTTCATGTGATTTCACTCTCCGTTGGTTCCAATGGTTATGCGCCTAATTATTATCGTGATTCAATTGCTATTGGTGCTTTTGGTGCTGCGCAACACGGTGTTGTTGTATCTTGTTCTGCTGGTAATTCAGGTCCTGGTCCTTATACTTCTGTTAACATAGCTCCTTGGATTTTAACAGTTGGTGCTTCGACTATCGATAGAGAGTTTCCTGCTGATGTTGTTCTTGGGGATGGTAGAGTTTTCGGCGGTGTTTCTTTGTATTATGGTGATGATTTACCGGATTATAAGCTTCCTTTAGTTTATGCTAGTGATTGTGGTAGTAGATATTGTTACATAGGTTCTTTAGATTCTTCGAAAGTGAAAGGGAAGATTGTTGTTTGTGATCGAGGAGGGAATGCTAGAGTTGAGAAGGGTAGTGCTGTGAAAAGTGCTGGTGGGTTAGGGATGATTATGGCGAATACTGAAGATAGCGGTGAAGAGCTTCTTGCTGATGCACATCTTGTTGCAGCTACAATGGTTGGTCAAATTGCTGGTGATAAGATTAAAGAGTATATAAGATCGAGTGAGAATCCAACAGCTACGATCGAGTTTAAAGGAACTGTGATCGGTGGTTCGGAGTCACCTTCTGCTCCTAGAGTTGCTTCATTTTCTAGCAGGGGTCCAAATTATAGAACTGCAGAGATTCTTAAACCAGATGTTATAGCTCCTGGTGTTAACATTTTAGCAGGGTGGACTGGTAAAGTGGGTCCTACTGATTTGGATTTGGATCCTAGGAGAGTTGAGTTTAACATAATTTCAGGTACTTCTATGTCGTGTCCTCATGTTAGTGGAATTGCTGCATTGCTTAGGAAAGCTTATCCGAATTGGTCACCTGCTGCGATTAAATCGGCTTTGATGACAACTGCTTATAATGTTGATAACTCCGGCGAAGCAATTCAGGATCTTGGGACAGGTAAAGAGTCAAATCCATTTGTTCATGGAGCAGGACATGTTGATCCTAACAAAGCACTTAATCCCGGGTTGGTTTACGATTTAAACAGCAATGATTATTTAGCATTCCTTTGTTCAATTGGTTATGATGCTAGGAAAATTCAGGTTTTCACTAGAGAACCAACTAGTTATAATGTTTGCGAAAACAAAAGGAAATTTTCTAGTCCCGGTGATTTGAATTACCCTTCGTTCTCTGTTGTGTTTGATGCAAACAATGAATTGGTTAAGTATAAAAGGGTTGTTACTAATGTTGGTGGTTCTGTTGATGCTGTTTACACTGTCAAAGTGAATGCTCCTTTTGGTGTTGATGTTAGTGTTTCACCTAGCAAGCTTGTTTTCAGTAGTGAAAACAAAACTCAAGCTTTTGAGGTCACATTCGCCAGAATCGGATATGGTGGTTCTCAGAGTTTTGGGTCTTTGGAATGGAGTGATGGAGTTCATATTGTGAGGAGCCCAATTGCTGCTAGATGGAGCAATGGATTCTCATCAGCATCCTTTTGATTTTTCATTCTGTGAAAGTGGAAACACATTATTATTATTACCATGCATAGGTGGAAACATGTTATGTATGAAGTTAATTAGTCTTGTATTGTTGTAACTTTTATGTTAGCTTTTATTGTACAAACTAAACAAGTAAAATTATTTGGTTTGGTTGTTTCTGTTTTNTRATGTTGTATTAGTATCTAATTTCTAAGTAG
TARGET=2024,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03319 Ps_contig_mira-and-tgicl-ass_franssen_29509
SEQUENCE=TCGGGAAGATCCTATTTTTCAGTTTATTATGAAAATTTGTGTTGGGAANRRGGACCTTGTCACCATTTTCAAGTTACAGAGAGAGAGAAAGTAATGGCAACAATGCAAGAGTGCCCAAGGAGTCCAGAAGCGAAACTTGGGATGCGAGTTGAAGACCTATGGGATGGTCAAGAGGCACAATTGAGTCCTACAGAAAAGCTTAACGCTTGTTTTGAAAGCATCCCTGTTTCTGCTTTTCCTTTACCATCTTCAAACCAAGGAAGTTGAAATAAAATCAGATGCAACTTTAGCTGAAGCTGTTAAAATACTGGCACATCATAATATTCTGAGTGCACCTGTGGTGGATGTTGATGCACCTGAAGATGCTACTTGGATTGATAGATATATTGGGATTGTTGAGTTTGCTGGAATTGTTGTATGGATTTTGCATCAGTCTGAACCTGCATCTCCTAAGAGTCCATCCACTCCAACCAGTGCAAGTGCTATTGCGGCCGCGGCAAACGGAAATACTTTTGCTCTTGAACTCGAAGCATTAGGCATTGGATCTGCCGCCACAACTTCCGGAGACTTTTTCGAGGATCTTACTTCTTCTGAACTTTATAAGAGCACCAAGGTTCGGGATATTTCAGGGACGTTCCGATGGGCTCCGTTTCTTGCTTTGGAGAGATCAAACTCATTTCTAACCATGCTCTTGTTGCTTTCTAAGTACAAGATGAAGAGTGTTCCTGTTGTAGACTTAGGTGCTGGTAGAATTGATAACATTATCACACAAGCCGCCGTGATTCATATGGTTGCAGAATGTGCCGGACTTCAGTGGTTCGAAAATTGGGGAACCAAGGAACTATCTGATGTTGGTCTTCCTCTTGTGTCACCAACGCAAATTATCAAGGTTTATGAGGATGAACCGGTACTTCAAGCATTTAAAGTGATGAGGAAAAAGAGGGTTGGAGGAGTGCCTGTCATCAAAAGAGGCGGCAACATGCCAATTGGTAATATAAGCTTGAGAGATGTTCAATTCTTGCTAACAGCCCCAGAAATCTATCACGATTATAGGGCTATTACAGTGAAGGATTTTATGACTGCTGTTAGCAGCTACTTAGAGAAGAATAAAACCACATTCTCAATGTCATGCGAATTCGTTACATGCAAAAAGAACTGTACCGTCAAAGAGTTGATTCAATTGCTTGACAGAGAGAAGATTCATAGAGTCTACGTCGTGGACGACGACGGGGACCTTGAAGGACTAATCACACTAAGAGATATCATCTCAAGGCTAGTACATGAGCCCCATGGTTATTTTGGCGATTTCTTTGATGGTGTTCTCCCTTTACCGGCTAACAGCAGGGTTTAACAGACAATGAATCCAAGAAGAATAATTTGCTCTTGCAAAGATTTGTTTTTGTCTTTTTTTAATAATTCTTAAGTGAAGAAGAAAAGTGAAAAATATGTATTTATCTTGAGGGTTGAAGAGTGTACCTAGGTTTTTGTAATGTGTAGGCAATTTGCAAGTTACTTGAACCTGTTTTAACTAACGTTAGAATGGGACTTTATTTACTGTAAATTCTGGTGTAACCACAAATTCTGTCAAATTTTGAGTTGTTTTTGTTCCTGTCTGTGTTCCGTTGTTATGATTTGAAATAGTACATAGTGCAACAAAAGAGGAAATATATGTGTTCCTAAAAGTT
TARGET=78,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03320 Ps_contig_mira-and-tgicl-ass_franssen_29510
SEQUENCE=AAAAATGACCTNGTCACCATTTTTCAAGTTACAGAGAGAGAGAGAAAGTAATGGCAACAATGCAAGAGTGCCCAAGGAGTCCAGAAGCGAAACTTGGGATGCGAGTTGAAGACCTATGGGATGGTCAAGAGGCACAATTGAGTCCTACAAGAAAAGCTTAACGCTTTGTTTAGAAAGCACTTCCCTTGTTTTNTCTTTTCCTTACNTCTACAACA
TARGET=33,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2886
PRIMER_LEFT_PENALTY=1.115242
PRIMER_RIGHT_PENALTY=0.173354
PRIMER_LEFT_SEQUENCE=GACCTNGTCACCATTTTTCAAG
PRIMER_RIGHT_SEQUENCE=CCATCCCATAGGTCTTCAACTC
PRIMER_LEFT=6,22
PRIMER_RIGHT=123,22
PRIMER_LEFT_TM=58.885
PRIMER_RIGHT_TM=59.827
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.50
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=118
PRIMER_PAIR_PENALTY_1=1.2886
PRIMER_LEFT_1_PENALTY=1.115242
PRIMER_RIGHT_1_PENALTY=0.173354
PRIMER_LEFT_1_SEQUENCE=GACCTNGTCACCATTTTTCAAG
PRIMER_RIGHT_1_SEQUENCE=CTTGACCATCCCATAGGTCTTC
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=128,22
PRIMER_LEFT_1_TM=58.885
PRIMER_RIGHT_1_TM=59.827
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.50
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=123
PRIMER_PAIR_PENALTY_2=1.2886
PRIMER_LEFT_2_PENALTY=1.115242
PRIMER_RIGHT_2_PENALTY=0.173354
PRIMER_LEFT_2_SEQUENCE=GACCTNGTCACCATTTTTCAAG
PRIMER_RIGHT_2_SEQUENCE=TCTTGACCATCCCATAGGTCTT
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=129,22
PRIMER_LEFT_2_TM=58.885
PRIMER_RIGHT_2_TM=59.827
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.50
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=1.4190
PRIMER_LEFT_3_PENALTY=1.115242
PRIMER_RIGHT_3_PENALTY=0.303792
PRIMER_LEFT_3_SEQUENCE=GACCTNGTCACCATTTTTCAAG
PRIMER_RIGHT_3_SEQUENCE=GACCATCCCATAGGTCTTCAAC
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=125,22
PRIMER_LEFT_3_TM=58.885
PRIMER_RIGHT_3_TM=59.696
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.50
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=1.4804
PRIMER_LEFT_4_PENALTY=1.307032
PRIMER_RIGHT_4_PENALTY=0.173354
PRIMER_LEFT_4_SEQUENCE=GACCTNGTCACCATTTTTCAAGT
PRIMER_RIGHT_4_SEQUENCE=CCATCCCATAGGTCTTCAACTC
PRIMER_LEFT_4=6,23
PRIMER_RIGHT_4=123,22
PRIMER_LEFT_4_TM=59.693
PRIMER_RIGHT_4_TM=59.827
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.50
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_03321 Ps_contig_mira-and-tgicl-ass_franssen_29526
SEQUENCE=TGGTATCAACGCAGAGGTACCGGGGGTTCAAAAGCATCTACTTAGTATGGCTTTCCCAATGTCTCATTTCTTGTTACTTTCTCTTCTTGTTGGTCTCTTTTTCTTTTGCTTTTTAGAGAAAGGTTTTGCCATTGAAGAACCTTCATGGGAAGAAAGACACCATCTTGTTCAAATCAACTCTCTCTTACCATCATCTTCTTGCAGTTCTTCAACCAAAGGTCCAAAAAGAAAAGCATCATTAGATGTATTACACAAACATGGCCCATGTTCCCAACTGAATAACAATGGAAAAGCAAATTCTATTCCAACACATAGTGATATTCTAAATCATGACAAAGAAAGAATAAGCTATATTCACTCAAAGCTATTATCATCATCCAGTAACAATAACAACAATAAGGTGGAAATAGAATTGGATTCTTCTTCTTCAGCTAGTTTACCTGCAAAATCTGGTAGCTTAATTGGATCAGGAAACTATTATGTTGTTCTTGGACTTGGTACTCCTAAAAAAGATTTATCACTTATTTTTGATACCGGTAGTGATCTTACTTGGACTCAATGTCAACCTTGTGCTCGTTCTTGTTATAAACAAATAGATGAAATATTTGATCCATCAAAGTCTACTTCTTATTATAATATCACATGTACTTCTCAAGATTGTACTCAACTCTCTTCAGCTACAGGAAATGATCCCGGCTGTTCTTCATCGACAAAAGCATGCATATATGGGATCCAGTATGGCGACCAATCTTTCTCGGTCGGATACTTCAGCCGCGAGCGGCTGACTGTAACATCGACAGATGCTGTCGATTGGTTTTTTATCGGTTGTAGGAACCAAAAACCAACCAAGGCCTTAGTTCCGGGCGGCTCGACGGCCGGTNTGGTTACGGCCTTGG
TARGET=418,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2904
PRIMER_LEFT_PENALTY=0.008292
PRIMER_RIGHT_PENALTY=1.282148
PRIMER_LEFT_SEQUENCE=CTTCAACCAAAGGTCCAAAAAG
PRIMER_RIGHT_SEQUENCE=TCCTGATCCAATTAAGCTACCA
PRIMER_LEFT=206,22
PRIMER_RIGHT=471,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=58.718
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=1.2943
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=1.282148
PRIMER_LEFT_1_SEQUENCE=AGTTCTTCAACCAAAGGTCCAA
PRIMER_RIGHT_1_SEQUENCE=TCCTGATCCAATTAAGCTACCA
PRIMER_LEFT_1=202,22
PRIMER_RIGHT_1=471,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=58.718
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=1.3702
PRIMER_LEFT_2_PENALTY=0.008292
PRIMER_RIGHT_2_PENALTY=1.361920
PRIMER_LEFT_2_SEQUENCE=CTTCAACCAAAGGTCCAAAAAG
PRIMER_RIGHT_2_SEQUENCE=CCTGATCCAATTAAGCTACCAGA
PRIMER_LEFT_2=206,22
PRIMER_RIGHT_2=470,23
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=59.638
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=1.3702
PRIMER_LEFT_3_PENALTY=0.008292
PRIMER_RIGHT_3_PENALTY=1.361920
PRIMER_LEFT_3_SEQUENCE=CTTCAACCAAAGGTCCAAAAAG
PRIMER_RIGHT_3_SEQUENCE=TCCTGATCCAATTAAGCTACCAG
PRIMER_LEFT_3=206,22
PRIMER_RIGHT_3=471,23
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.638
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=1.3741
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=1.361920
PRIMER_LEFT_4_SEQUENCE=AGTTCTTCAACCAAAGGTCCAA
PRIMER_RIGHT_4_SEQUENCE=CCTGATCCAATTAAGCTACCAGA
PRIMER_LEFT_4=202,22
PRIMER_RIGHT_4=470,23
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.638
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_03322 Ps_contig_mira-and-tgicl-ass_franssen_29544
SEQUENCE=ATTTTTTAAGTAGAATCATACTCTAAGCTTTTTTATTTTGTAACAATCTCAAACAATTTTGCTTTGATTACATTAAACTTTCTCTATAGGGAACAATAATCAATTAATTTGACATTTCCAATAGAAACTTATTGAATTTTTAATTTATAACTTGATTACAATAACTTCAGATTAAAACTAAGGATAAACTTCAATGAGAGACTTGAAGCCACATATAGGAGTAATTTTGTAGCGGCTGAAACCTGCATCATATATGAGTTTCTCCCATTCTTTCTTTGTCCTTTCTTTACCAAGAAACATTGTTAGCATCACTACATCATACTCCAATTGCAACTCAGTTAATCCATGATCATCACTGTTGTCATCAATTGATATGTCTATGATAATCACTTTTCCATCTTTCCCTTTGTGTGAAATTGCTTCTTTGCTTTTCTTCAATATCTTTAAAGATAGTTCATCATTCCAATCATGCAGAACCCACTTGAGTAAAACAGCATCAGCAGAAGGGACAGATTTGAACATATCTCCACCAACAAAATTCAAATTTTCATTTCCAGTTAAGTTACCAACAACCTGTGGCTGATCAAAAACGGTGCATTTGATGTGAGGAAATGCTTCATGAATGAGTTTTGCAACACCACCAGTTCCACCTGCCACGTCAACCAAAGACTCCAAACCCTCAAACACATGCTTATTCTCTTGAATAGCAAGCTTAAACAATCTTGAATCAGCCGCCATAGCATCTTGAAATATACTCAAATAATCAGAATCTTTGTTAAGAAAATCCCAATAGTTCTCCCCAGTTGCACACTCAAAAAGTGTTTGTTCCTTATCCTCATGGAACCATTTCTTTGAAACACCCCACAAGTCTAAAGAGATTGGATGAAGTGCTCCTTTAACAACTGATGATAAACATGTTGATTTACCACTTACGAGAAGCTTTGAAGAAGGTGTGAGAACATACGCTGTTTCTTCTTCTTCTCCATTGCTTTTCACTGTTGTTTTAGCAAAGAAACCATTGTATGTTAACAGACGTAAGAATCGATAAAGGATGTTAACTTTAGAAGGGTGAAGTTTCAAAGATGAAGCTAATTCAGGGAGAGTCATTGGTTTTCCATGGTTATGAATTGCATCAGCTATGCCTAGTTCCACGGCGGATTTGAGTGCCATGGAACTTACAAAGTTGTATATATGTTTGTATAAATGAATTTGAGCATGATACAGTTCACTTTCTTCAGAACCGTTTGTACTGAAATCCATTTCTATTGTTTGTTAACTTGGGTGGTTTGGTCGAAATGGGTATCCCCCGAACTTATAAACCAGTATGGTACCTTTTTATATGTTTCATATGTTGAACG
TARGET=970,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0776
PRIMER_LEFT_PENALTY=0.054021
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=CGAGAAGCTTTGAAGAAGGTGT
PRIMER_RIGHT_SEQUENCE=AAAACCAATGACTCTCCCTGAA
PRIMER_LEFT=932,22
PRIMER_RIGHT=1113,22
PRIMER_LEFT_TM=60.054
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=0.0776
PRIMER_LEFT_1_PENALTY=0.054021
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=ACGAGAAGCTTTGAAGAAGGTG
PRIMER_RIGHT_1_SEQUENCE=AAAACCAATGACTCTCCCTGAA
PRIMER_LEFT_1=931,22
PRIMER_RIGHT_1=1113,22
PRIMER_LEFT_1_TM=60.054
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.1496
PRIMER_LEFT_2_PENALTY=0.054021
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=ACGAGAAGCTTTGAAGAAGGTG
PRIMER_RIGHT_2_SEQUENCE=AACCATGGAAAACCAATGACTC
PRIMER_LEFT_2=931,22
PRIMER_RIGHT_2=1121,22
PRIMER_LEFT_2_TM=60.054
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.1496
PRIMER_LEFT_3_PENALTY=0.054021
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=CGAGAAGCTTTGAAGAAGGTGT
PRIMER_RIGHT_3_SEQUENCE=AACCATGGAAAACCAATGACTC
PRIMER_LEFT_3=932,22
PRIMER_RIGHT_3=1121,22
PRIMER_LEFT_3_TM=60.054
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=190
PRIMER_PAIR_PENALTY_4=0.2105
PRIMER_LEFT_4_PENALTY=0.186949
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=TGTTCCTTATCCTCATGGAACC
PRIMER_RIGHT_4_SEQUENCE=AAAACCAATGACTCTCCCTGAA
PRIMER_LEFT_4=823,22
PRIMER_RIGHT_4=1113,22
PRIMER_LEFT_4_TM=60.187
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_03323 Ps_contig_mira-and-tgicl-ass_franssen_29545
SEQUENCE=CCTTAATTTTGCTACCTTATAAAGGTGGCATGGATAGTAGCAAATGACACCAGAGTTCGAGTAGAAGCCTATCATGAATGACGACGTCAAAAGATATTGAAGCATAATGATTAGGCCAGCCATAAAACCAAGTCAAATAAAAACACTACAAGCATAAACTTGAGTACAACCAGTTACACTGTTTTACAAGACTTTATATGCCTTCTCATTTCAAGATCGGCCATAATTTGGTATGACCCAACCCTTATTCTCGTGTGAATTTTTAGATGCCTATCACAGTTTTGCAGGAGGCTGGTACCAGCCCCATGGTTCCTCAGAGATATACTGAGTCACTTGCTTCACGCTCAAGTAATTGTCAAGTCCCCATTCTCCTAGTTCACGGCCAAAACCACTACGTTTGACGCCTCCCCATGGAGCTTGAGTGAAGCATGGTTGAGAGCAATTGACCCATACAATTCCGGCCTTAAAAGCCTTAGTAACGCGTTCACATCTTTCTAGATCATTTGATATTACAGCAGCACCTAAGCCATAGACAGTGTCATTTGCTAGATCAATAGCTTCTTCTTCAGTGCTAAATGTTTTTACACAGAGGACAGGTCCAAATACTTCTTCTCTCCAAATTTGCATGTTAGTAGTCACGTCGGTTATGATGGTTGGTTCAATAAAGAATCCTTTCTTTAGATGCTCTGGTCGAGACCCACCAGTCAAAATGGTTGCACCCTCGCTCTTAGCATTTGAGACAAACTTCAATATTTTTTCATACTGTCCTTCACTAACAACAGGTCCTAGTCTGCAACCTTCTTCCAAGGGATCTGAAATTTTGATGTTTTTGATCCATTTGACAATCCTATTCAAAAACTCTGTTGCTATACTTTCATGTAAAATAAGACGGGAAGTTGCACTGCATATCTGACCATTTGTCCAGAAGCAGCCAAAGATGGCCCATTCAGCAGCCTTATCAAGGTCAACATCCTCAAAAACAACTAATGGGCTTTTTCCACCAAGCTCCAGTGAAACAGGCTTAACGAGCTGAGCTGCAGCTGTCATGATTTTGCTCCCAGTTGCAGAGCTTCCAGTAAACGCAACCTTGTCAACATCAGGATGCGTTGCCAAAGGAGCACCAGCTTCAGGACCTAATCCAGTGAGAATATTCAACACACCTGGAGGAAGGCCCACTTCTTTGCATATTTCGCCCAACTCCAAGCAGGTTAGAGATGCCAATTCAGACGGCTTCAATATTGCAGCACAGCCCGCAGCAAGAGCAGGAGCAACCTTCCACGTAGCCATCAACATAGGATAATTCCATGGAGTTATCAATCCAACAACACCAATAGGTTCCCTAAGCACATGACTCTTGAATGTATCCATTGGAAGAGACACCGGAGCCTTTTGTCTTGCATCCAATTTTTCAGCAAGATCAGCATAATACTCAAAGCAACCAGCAACATCATCAATGTCCCACGCGGCTTCATCGAGTGGTTTTCCACAATCAATAGACTCAAGCTTCGCTAGTTCAGGCTTTTTCTCAGTAACCTTAGCGGCAATGGCGCGTAGATAGCGTGCACGAACAGCGCCAGAAGCGGTAGCCCAATCAGCGCCTTTGTTACGCGTGAGAGCAGTTTTAGCGGCGGCGACGGCGACGTCGACGTCTTCCTTAGTAGCGGCAGGGATATCGCCGATGATGTTTTGGGTGGCGGGATTGATGACGGGAATCCGTTTGTTAAGGACGGGAGCTTTCCAGTCGCCGTTAATGAACAACTGACGAGTTGGGATCGGAATATCCATTCTAGTACTTTCTCTCTCCAATCTATTATTGATTTGATGGATATGGAGATAGATAGATCAGATCTCACCTTGAAAGTTGCTGAAATTGTTCACTCAACTGAGCTAAGATAAAGTGTTATTCTCTACGGCCCCCGAACTCTGCGTTGAT
TARGET=1832,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7378
PRIMER_LEFT_PENALTY=0.272307
PRIMER_RIGHT_PENALTY=0.465503
PRIMER_LEFT_SEQUENCE=TTACGCGTGAGAGCAGTTTTAG
PRIMER_RIGHT_SEQUENCE=TTTCAGCAACTTTCAAGGTGAG
PRIMER_LEFT=1603,22
PRIMER_RIGHT=1869,22
PRIMER_LEFT_TM=59.728
PRIMER_RIGHT_TM=59.534
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.7944
PRIMER_LEFT_1_PENALTY=0.328883
PRIMER_RIGHT_1_PENALTY=0.465503
PRIMER_LEFT_1_SEQUENCE=CCGTTAATGAACAACTGACGAG
PRIMER_RIGHT_1_SEQUENCE=TTTCAGCAACTTTCAAGGTGAG
PRIMER_LEFT_1=1744,22
PRIMER_RIGHT_1=1869,22
PRIMER_LEFT_1_TM=59.671
PRIMER_RIGHT_1_TM=59.534
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.8640
PRIMER_LEFT_2_PENALTY=0.398516
PRIMER_RIGHT_2_PENALTY=0.465503
PRIMER_LEFT_2_SEQUENCE=GTTACGCGTGAGAGCAGTTTTA
PRIMER_RIGHT_2_SEQUENCE=TTTCAGCAACTTTCAAGGTGAG
PRIMER_LEFT_2=1602,22
PRIMER_RIGHT_2=1869,22
PRIMER_LEFT_2_TM=59.601
PRIMER_RIGHT_2_TM=59.534
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.9096
PRIMER_LEFT_3_PENALTY=0.444125
PRIMER_RIGHT_3_PENALTY=0.465503
PRIMER_LEFT_3_SEQUENCE=GTCGACGTCTTCCTTAGTAGCG
PRIMER_RIGHT_3_SEQUENCE=TTTCAGCAACTTTCAAGGTGAG
PRIMER_LEFT_3=1641,22
PRIMER_RIGHT_3=1869,22
PRIMER_LEFT_3_TM=60.444
PRIMER_RIGHT_3_TM=59.534
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=0.9096
PRIMER_LEFT_4_PENALTY=0.444125
PRIMER_RIGHT_4_PENALTY=0.465503
PRIMER_LEFT_4_SEQUENCE=CGTCGACGTCTTCCTTAGTAGC
PRIMER_RIGHT_4_SEQUENCE=TTTCAGCAACTTTCAAGGTGAG
PRIMER_LEFT_4=1640,22
PRIMER_RIGHT_4=1869,22
PRIMER_LEFT_4_TM=60.444
PRIMER_RIGHT_4_TM=59.534
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_03324 Ps_contig_mira-and-tgicl-ass_franssen_29546
SEQUENCE=AACGCAGAGTACGTCGGGGACACTTACTCATTTAGATCCTAAGTTTCCTAATTTCACCTTACTCATAAATTTTCTCTTCACTAATTTCATTTTTGTTGGATTAAAAAAAGTGTTTTGCCCTAATCATAGTAAACATATGGCTATGGGTTCTTTTCAAAATTTCTTCTTCTTCCTTCTTATCATCTCTTGCTACATCCTCACAATCTTCAGTGAACAAGAGGAGACCGGTTTTGTGAGCTCAATAAATCCTAAGTTGTTTAAAAAGAAAGAAAATTTTAGTCATTTTAGATTCTACTGGCAAGACATTGTTGGAGGAAACAACGCTACGTCCATTCCAATTATTCCACCTCTCCCCAAATTCGACAACGATTATAGTGTTTTTGGTTTAGTGAGGATATTTGACAACGCTTTAACGTTAGGACCAAAATTGAGCTCGAAGTTGTTAGGCAGGGCACAAGGGTTTTTGGCATCTACCTCACAAACTGAGCTCGATTTTCTTATGGTTATGAACTTTGCTTTGTTTGAAGGAAAATATAATGGGAGTGTCATCACTATCTCTGGAAGAGACGTTGCCTATAATAAGACCAGAGAAATGTCTGTAATTGGTGGGAGTGGTGTTTTTCGATTTGCTAAGGGATATGCTGAAGCTAACACAATCTCTTATGATCCTATAACGGTGAATACTATTGTTGAATACAATGTCTTTGTTTCTCATTAGGATTGATGTTTTTCGTTTTGTTGTTAATAACTAGTGATAACATTCATCTTTGCATGTTTTTTCATGTGGTTGATGTCGTCATATGTGATATGTATCCTTGTCATCTTTGCATTGTTTTTTCATGTGAGGGTTGATGTTGTCATATGTGTCCATATTTATTGGTGTGTAATTGTAATCCGTATCATGATTAGAATAATAAAACTTTGTATTATTAACG
TARGET=161,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1759
PRIMER_LEFT_PENALTY=1.007752
PRIMER_RIGHT_PENALTY=0.168114
PRIMER_LEFT_SEQUENCE=TCGGGGACACTTACTCATTTAGA
PRIMER_RIGHT_SEQUENCE=TCCAACAATGTCTTGCCAGTAG
PRIMER_LEFT=13,23
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.168
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=1.1759
PRIMER_LEFT_1_PENALTY=1.007752
PRIMER_RIGHT_1_PENALTY=0.168114
PRIMER_LEFT_1_SEQUENCE=TCGGGGACACTTACTCATTTAGA
PRIMER_RIGHT_1_SEQUENCE=CCAACAATGTCTTGCCAGTAGA
PRIMER_LEFT_1=13,23
PRIMER_RIGHT_1=311,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.168
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=299
PRIMER_PAIR_PENALTY_2=1.4544
PRIMER_LEFT_2_PENALTY=0.430652
PRIMER_RIGHT_2_PENALTY=1.023740
PRIMER_LEFT_2_SEQUENCE=AGTACGTCGGGGACACTTACTC
PRIMER_RIGHT_2_SEQUENCE=ACAAAACCGGTCTCCTCTTGT
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=233,21
PRIMER_LEFT_2_TM=59.569
PRIMER_RIGHT_2_TM=60.024
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=1.4544
PRIMER_LEFT_3_PENALTY=0.430652
PRIMER_RIGHT_3_PENALTY=1.023740
PRIMER_LEFT_3_SEQUENCE=AGAGTACGTCGGGGACACTTAC
PRIMER_RIGHT_3_SEQUENCE=ACAAAACCGGTCTCCTCTTGT
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=233,21
PRIMER_LEFT_3_TM=59.569
PRIMER_RIGHT_3_TM=60.024
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=1.5408
PRIMER_LEFT_4_PENALTY=0.430652
PRIMER_RIGHT_4_PENALTY=1.110099
PRIMER_LEFT_4_SEQUENCE=AGAGTACGTCGGGGACACTTAC
PRIMER_RIGHT_4_SEQUENCE=TGTGAGGATGTAGCAAGAGATGA
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=201,23
PRIMER_LEFT_4_TM=59.569
PRIMER_RIGHT_4_TM=59.890
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=197
=
PRIMER_SEQUENCE_ID=SSR_03325 Ps_contig_mira-and-tgicl-ass_franssen_29548
SEQUENCE=TTGAAACAAAACAGCATTTGAAGAAGTTGTTTAGTTCCAAGTAACTTTAAAAAAAAAAGTTTAGGTTAACTACTTTCTGAGGTACTAACACCAGTTCTACATGAAAATAGATTGATGAATGAAAATTACATTGGCTTCACAAATTCCATTTACTTCCATAACTCTTCCTTAGGGATCCCACGCTCTTCGGCGACCTGACTGTACTGCTTCATTTTCTGCACTGCAGCTACAGTTGCTCTGGCATTGTTGAGTGCATTGTCACTTCCGAGTTGTTTCCCCAAAGCATTTTCAACACCTGCCAGTTCAAGAACAATTCTCACAGAACCACCTGCAATAACTCCGGTACCAGGTGAAGCGGGTCTAAGCATCACCTTTGCTGCTCCATAATCTCCGTCTGATCTATGAGGAAAAGTGGAGTACTTAGTCATGGGGACTCTAACAAGATTCCTCCTAGCATTAACAGCAGCTTTCTGAACAGCAGAAACAACTTCCTTAGCCTTCCCAAGGCCAACACCAACTTGTCCTTTCTTATCACCAACAACAACAACAGCCCTAAACCTCATCTGTTTCCCTCCTTTAACAACCTTAGTAACCCTTCTCACTTGAACAACTCTTTCTTCCATTCCATCTCTAAACCTCTCTTTCTTCTTCGCTCCAGAGCCAAACCCAGTAGCCTTCCTCTTCTTCGCATCCATGGCGTATATATCATTTCCGAGAACACTCACTCCACTGTACCCCACGCCGTAAATCTTTTCATACGCGGCGGCGATTTCGTCTTCGTCTTCGACTGGTCCGTCGTCTATGGAAGGTGGGGCGATGAAGCCTTCCGGGACTTCGGGTGGGTTGAATGGAATGACTTCTTCGGAGATAGGTGCGTTGTCGTCGAAGGAGTTAGGTGCGTTGTCGTCGAAGGAGTTGGCTTTGGCGGTGAAGGAGAGAGTGGTGGTTGTGGAGATGGAGAGTGGGGTTTTGGTGAGGAGAGGGAATGTGAGTTTGTTGGTTGTGGGGAGGAGAGAGAAACAAGAGGAGGAAGAAGAGGACCGGAAAGAGAGAGAAGAGAAAGAACTGAGAGCTGAAGAATGTGCAGAAGCCATTGAATTGAACAAGAGAGGAAGACGATGCCTCACTCTGAAAGGAAGTATTGTTGAGCGGTTGTTGATGACATGAAACGAAAAAGATAAAAGGATGCAAAAACTATCCAGAGATAAGGTTTTTTCTTTTAGATGGGATATGCTGGACTCATTTTATTTT
TARGET=536,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1040
PRIMER_LEFT_PENALTY=0.004697
PRIMER_RIGHT_PENALTY=0.099308
PRIMER_LEFT_SEQUENCE=AAACAACTTCCTTAGCCTTCCC
PRIMER_RIGHT_SEQUENCE=CTCTGGAGCGAAGAAGAAAGAG
PRIMER_LEFT=481,22
PRIMER_RIGHT=659,22
PRIMER_LEFT_TM=60.005
PRIMER_RIGHT_TM=59.901
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.1063
PRIMER_LEFT_1_PENALTY=0.006951
PRIMER_RIGHT_1_PENALTY=0.099308
PRIMER_LEFT_1_SEQUENCE=ATCACCTTTGCTGCTCCATAAT
PRIMER_RIGHT_1_SEQUENCE=CTGGAGCGAAGAAGAAAGAGAG
PRIMER_LEFT_1=366,22
PRIMER_RIGHT_1=657,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.1063
PRIMER_LEFT_2_PENALTY=0.006951
PRIMER_RIGHT_2_PENALTY=0.099308
PRIMER_LEFT_2_SEQUENCE=ATCACCTTTGCTGCTCCATAAT
PRIMER_RIGHT_2_SEQUENCE=CTCTGGAGCGAAGAAGAAAGAG
PRIMER_LEFT_2=366,22
PRIMER_RIGHT_2=659,22
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=294
PRIMER_PAIR_PENALTY_3=0.3356
PRIMER_LEFT_3_PENALTY=0.004697
PRIMER_RIGHT_3_PENALTY=0.330865
PRIMER_LEFT_3_SEQUENCE=AAACAACTTCCTTAGCCTTCCC
PRIMER_RIGHT_3_SEQUENCE=GGGGTACAGTGGAGTGAGTGTT
PRIMER_LEFT_3=481,22
PRIMER_RIGHT_3=737,22
PRIMER_LEFT_3_TM=60.005
PRIMER_RIGHT_3_TM=60.331
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.3470
PRIMER_LEFT_4_PENALTY=0.247716
PRIMER_RIGHT_4_PENALTY=0.099308
PRIMER_LEFT_4_SEQUENCE=ACCTTTGCTGCTCCATAATCTC
PRIMER_RIGHT_4_SEQUENCE=CTGGAGCGAAGAAGAAAGAGAG
PRIMER_LEFT_4=369,22
PRIMER_RIGHT_4=657,22
PRIMER_LEFT_4_TM=59.752
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_03326 Ps_contig_mira-and-tgicl-ass_franssen_29567
SEQUENCE=AAGCATGGTAGTCAACGCAGAGTAACGCGGGGACGATAACGCAAGCTCAGAGAGAGAGAGAGAGAGAGACAGAGTTAGGGTTTTACAGATTCCAATGTCTCTCCCCTGAAAATCCGAATCTCTCACCGCCGAATCCATTTCCGATTCCGATCAATGGGATCGTCATCGGCGTTCTTCGTCATCTGCATTCTCCACTCTATCATCGCGATCACAAGTGGAGCTCTCATGATGTTCTACATGAAAGAAGTCTACACTTTCGGTCACGGTGTTCAAACGGCGACGAAGCTTCTCGGATCCACGCCGCACGATCAGCTCCTCATCAAAACCTCCGATTCGTTCTCCGGTTTGCTGCTGGTTGCTATTGGATTTCTCATTTTCATGGTTTCGTTTGTTAAGGATCGGGATTTTCAACACTTGTTTGCTAAGGGGTGTATTTTGTTGCATGTGTTTATGGCGTTGTGGAGGATCTACTTTGAAAGGAAGGTTGAAGATCTTGCTTGGGATTGGCTTAGACAAACTGTTGGTGATGTTCTCTTGGCACTTTCGTGGGTTTTCTTCCTTATTTACTCTTGGAGAGAGAAGTATGATTAGTTTTACTTTTCTTTCCTTTTTTTTTCTTGTTTATTTTCTCCATCAGGATGATTATGATTATGAATTTTCTGTTTTTAATTTACTCTGTAAAAATCAGACTGATATCACATGTTTTTTTATTTATCAAGTGAGTATTTAAGCTGTGTATTACCTTACCTTGAGTTCAATTAGACTGAAAATTGCAGTAATGGAGCTTCTTCATTGACTTGTTAGTTTGGTTTGTAACTTCATTATGTCAAATTTGAATTGAGTTGTTCATTGAATGGTTTCCTTTTGTAATTCTGAGTTGGAGTTTTATACCTGAGTGTTTTCTTTTAATGACTATGAAAGTTGATTT
TARGET=49,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4194
PRIMER_LEFT_PENALTY=0.359545
PRIMER_RIGHT_PENALTY=0.059875
PRIMER_LEFT_SEQUENCE=TGGTAGTCAACGCAGAGTAACG
PRIMER_RIGHT_SEQUENCE=GATTTTCAGGGGAGAGACATTG
PRIMER_LEFT=5,22
PRIMER_RIGHT=113,22
PRIMER_LEFT_TM=60.360
PRIMER_RIGHT_TM=59.940
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=0.4340
PRIMER_LEFT_1_PENALTY=0.359545
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=TGGTAGTCAACGCAGAGTAACG
PRIMER_RIGHT_1_SEQUENCE=GTGAGAGATTCGGATTTTCAGG
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=125,22
PRIMER_LEFT_1_TM=60.360
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=121
PRIMER_PAIR_PENALTY_2=0.4854
PRIMER_LEFT_2_PENALTY=0.359545
PRIMER_RIGHT_2_PENALTY=0.125826
PRIMER_LEFT_2_SEQUENCE=TGGTAGTCAACGCAGAGTAACG
PRIMER_RIGHT_2_SEQUENCE=TCATGAGAGCTCCACTTGTGAT
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=228,22
PRIMER_LEFT_2_TM=60.360
PRIMER_RIGHT_2_TM=59.874
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.4854
PRIMER_LEFT_3_PENALTY=0.359545
PRIMER_RIGHT_3_PENALTY=0.125826
PRIMER_LEFT_3_SEQUENCE=TGGTAGTCAACGCAGAGTAACG
PRIMER_RIGHT_3_SEQUENCE=ATCATGAGAGCTCCACTTGTGA
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=229,22
PRIMER_LEFT_3_TM=60.360
PRIMER_RIGHT_3_TM=59.874
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=1.0245
PRIMER_LEFT_4_PENALTY=0.359545
PRIMER_RIGHT_4_PENALTY=0.664955
PRIMER_LEFT_4_SEQUENCE=TGGTAGTCAACGCAGAGTAACG
PRIMER_RIGHT_4_SEQUENCE=GGATTTTCAGGGGAGAGACATT
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=114,22
PRIMER_LEFT_4_TM=60.360
PRIMER_RIGHT_4_TM=60.665
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_03327 Ps_contig_mira-and-tgicl-ass_franssen_29572
SEQUENCE=TTAGTAGGGAAGTAGTTGCTTTTCTACCACCCAAAAAATACCTAAATAGNTCCACCCTCGAAGCTGTGAATCAAANAGGTTGATAAGGAACTAAGACCAGAACTGGGAAATGAATTTCCTATCAATAAAGACTACATTTGTTTGGTCCCGTCGCCGCCCTCCAGCCCCTCGCAATTCCGGAGGCATCTTCATCTGTTTCTTTACCCCATCATTGATTTCTATGGCTCAGCCCCTGCAATTTTGGCAACACCTTCAACTGTTTCTTACCCAATAATTGACTTATCTGGCACAACCCGGGCTGTTCCCTCCAACCAGCAAGCCTCAACTGCGGATGCACTATTATCCTCTTTAGACAAGGATGGAAATGATTCTGTGGAAGAGGCCCTCCTTAAAGAGCTTGAGGAGATGGGTTTTAAGCAGATTGATTTAAACAAAGAGGTTCTGCGAATGAATGAGTACAATCTGGAGCAGTCAATAGATGAACTCTGTGGTGCTTCTGAGTGGGATCCTCTCCTTGAAGAGTTGCATGATATGGGTTTTTGTGACAGAGAGACTAACAAGAGGCTGCTGTTGAAGAACGAAGGAAGCATTAAGCGTGTTGTTATGGACTTAATCAATGGAGAGTAGGTTTCATTATGTATTAGCTATTATATTATGGATGTTTAATTAAGTAATACGTTCAGGGAGAACGTTGAATAATCGCTAGGCACACTGCTATCTATTTTCCTTGACTCGATCTTGTGTTGTGGTGCTATGTCATTGCATATAGACAGTTGATCGATCTCATGTTTTATGCATATATATATATAATAAAAGACAAGACTATGTTCTATAATTTAATNAA
TARGET=797,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03328 Ps_contig_mira-and-tgicl-ass_franssen_29575
SEQUENCE=CTTTTTTAACATAAGGTTATNAACTAATAATTTGTAACAAAACTTTTAAATTTAGGGTCGTACATTTTCTTGTTCTCAAAGTAGCATTTTCCTTTTGCACAATACAAACATAACAAATTCACCCCCATTTCATTCTCAAATGAAGGGACCTTAATGGAAGTCAACAATTTTTCATACAAAGGCTATTAGGCCTAGACACTTGAATTAAAATTGATCTCCCTCACAAAAACCATATTAACCCATAGCATATTTCTGGGTCCAGCTTCTTGCAGTTGACTCGTACTTGTTCTTGTCTGTCTTGTACATGTGAGCGATTTCAGGAACCAGAGGATCATCAGGATTTGGGTCAGTCAACAAGGAGCAGATAGAGAGCAGCACCTTGGAAATGGTAAGGGCAGGGCTCCACTGCTCCTTTAGTATATCAAGGCAAATGCTTCCGTTGCTATTGATGTTTGGGTGAAATACCTTTGTCCTGAATGCAACCTTGGGTGGCTTAAAAGGATAATCTGGAGGGAAATGGATAGTGACAAGGAAAACACCTCCGGAATAAGGACTGTCTGGAGGACCCATAATTGTTGCTTGCCAATGAAACATATCTTCAGCAACAGGACCAGCACTGCATGAAGTTGGAGGATCCTTCTGCAAATCCTTGAGTTCTTTCAAGATCCGCTTCGAAGCCATAGCGTAAACCTAGGGAAAACGCGAGAGAGATCCTCCTTCGATTTTGCAACGAATAACGCGAAGACGATAGAAGGAATCTGTGATTGAAGAACGAAAAGGAGTTGTTGTGGTGTTGGTGCGTTTTCGGAATTTATGCACTTCCTTTCTTTTGTTCTGTGATTGGAATATTTATTTATT
TARGET=847,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_03329 Ps_contig_mira-and-tgicl-ass_franssen_29578
SEQUENCE=AAGCGTGGTATCAACGCAGAGRNTCGGGGATCATATTTCAACCATTCAGATATCTAGCGTAGCGTCACTTCAGTTTCATCCACTGCATCATCATCATCATCATCACCATGTGGCCATTCAGCAGCAAGAAAGGGGTTTCTGGATTTTCTGGTTCTTCAACTGCTGAACAAGTCACTCATGGAATCGATGCAACTGGCCTCACCGCCATTGTTACTGGAGCATCGAGTGGCATTGGCGCCGAGACTACTCGTGTGCTTGCTTTGCGCGGTGCTCATGTGATTATGGGTGTGAGAAATATGGTTGCTGCTAAGGATGTCAAAGATACAATACTCAAGGACATTCCCTCGGCTAAAGTTGATGCCATAGAGTTAGATCTCAGCTCATTGGACTCTGTCAAGAAATTTGCATCCGAGTTTAATTCCTCTGGCCGTCCACTGAACATCTTGATAAATAATGCAGGAATTATGGCATGCCCTTTCAAGCTGTCCAAAGACAACATTGAACTACAGTTTGCCACCAATCACATAGGGCATTTTCTTTTGACAAATCTTTTGTTGGATACTATGAAGAAAACGACACGTGAAAGTAAAAAAGAAGGAAGAATTGTTAATGTTGCCTCAGAGGCTCACAGGTTCGCATATCCCGAAGGAATCCGTTTTGACAAAATCAATGACCAATCAAGTTATAACAACTGGCGTGCATATGGGCAATCCAAGCTTGCTAACGTTTTACATGCCAACCAACTTACAAAACATCTCAAGGAAGATGGTGTGAATATTACTGCAAATTCTCTTCATCCAGGAACAATTGTCACCAACCTTTTCCGTCATAACAGTGCAGTGAACGGTCTAATAAATGTGATTGGGAAACTTGTGTTGAAAAATGTTCAGCAGGGAGCAGCTACAACATGCTATGTAGCATTGCACCCACAAGTGAAGGGAGTAAGTGGTGAATATTTTTCAGACAGTAATGTGTACAAGACAACACCACATGGGAAGGATGTTGATTTGGCAAAGAAACTCTGGGATTTCAGCATCAATTTGGTTAAGCAGAAATAGAATAACAAGTGTTAGTGTCAATGCTATACACTAAATGAAATGTATCAATAATATGATTGTCACAAATAAAATGCTTCAATAGAATCACTCTGTCACATCTATCTATCTATTCTACGAAAGATATTTGCACAANGAGTTTTTATACATCTATTTTTCTGTCATTTACTAGTTTTCTGTAGTGAGACTGAGTCCTACATTGCATAGACATCAATTTCAAGCTTTACTTTGAATGACAGTTCTATTTTTTGTAGAAAAGGTAAAAGT
TARGET=86,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03330 Ps_contig_mira-and-tgicl-ass_franssen_29578
SEQUENCE=AAGCGTGGTATCAACGCAGAGRNTCGGGGATCATATTTCAACCATTCAGATATCTAGCGTAGCGTCACTTCAGTTTCATCCACTGCATCATCATCATCATCATCACCATGTGGCCATTCAGCAGCAAGAAAGGGGTTTCTGGATTTTCTGGTTCTTCAACTGCTGAACAAGTCACTCATGGAATCGATGCAACTGGCCTCACCGCCATTGTTACTGGAGCATCGAGTGGCATTGGCGCCGAGACTACTCGTGTGCTTGCTTTGCGCGGTGCTCATGTGATTATGGGTGTGAGAAATATGGTTGCTGCTAAGGATGTCAAAGATACAATACTCAAGGACATTCCCTCGGCTAAAGTTGATGCCATAGAGTTAGATCTCAGCTCATTGGACTCTGTCAAGAAATTTGCATCCGAGTTTAATTCCTCTGGCCGTCCACTGAACATCTTGATAAATAATGCAGGAATTATGGCATGCCCTTTCAAGCTGTCCAAAGACAACATTGAACTACAGTTTGCCACCAATCACATAGGGCATTTTCTTTTGACAAATCTTTTGTTGGATACTATGAAGAAAACGACACGTGAAAGTAAAAAAGAAGGAAGAATTGTTAATGTTGCCTCAGAGGCTCACAGGTTCGCATATCCCGAAGGAATCCGTTTTGACAAAATCAATGACCAATCAAGTTATAACAACTGGCGTGCATATGGGCAATCCAAGCTTGCTAACGTTTTACATGCCAACCAACTTACAAAACATCTCAAGGAAGATGGTGTGAATATTACTGCAAATTCTCTTCATCCAGGAACAATTGTCACCAACCTTTTCCGTCATAACAGTGCAGTGAACGGTCTAATAAATGTGATTGGGAAACTTGTGTTGAAAAATGTTCAGCAGGGAGCAGCTACAACATGCTATGTAGCATTGCACCCACAAGTGAAGGGAGTAAGTGGTGAATATTTTTCAGACAGTAATGTGTACAAGACAACACCACATGGGAAGGATGTTGATTTGGCAAAGAAACTCTGGGATTTCAGCATCAATTTGGTTAAGCAGAAATAGAATAACAAGTGTTAGTGTCAATGCTATACACTAAATGAAATGTATCAATAATATGATTGTCACAAATAAAATGCTTCAATAGAATCACTCTGTCACATCTATCTATCTATTCTACGAAAGATATTTGCACAANGAGTTTTTATACATCTATTTTTCTGTCATTTACTAGTTTTCTGTAGTGAGACTGAGTCCTACATTGCATAGACATCAATTTCAAGCTTTACTTTGAATGACAGTTCTATTTTTTGTAGAAAAGGTAAAAGT
TARGET=1155,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03331 Ps_contig_mira-and-tgicl-ass_franssen_29580
SEQUENCE=TAAGTAGTTGGTTATTCTAACGCAGAGTACGCGGGGATTGACGAGTGACTTTTATTTAAACCTACTTCACTCCAAAACTATTCTGATTCATTCAACAACACCACCTCTCTCGCCTCTCCTTTTTCTCTGCTCAGTTTTTTCGCATCCATCCATCAGTAATGGCGTTGGAAGGTGTTCTCCTCGGTATGGGTAATCCCCTTCTCGACATTTCTGCTGTCGTCGACGAAGATTTCTTGAAAAAGTTTGATATTCAGTTGAACAATGCCATTCTCGCTGAAGATAAACACAAATCTATGTACGATGAAATGTCTGCCAAATTTAACGTGGAGTATATTGCTGGAGGTGCCACTCAGAATTCAATTAAGGTTGCTCAGTGGTTGCTTCAAGTACCCGGAGCCACTAGTTACATTGGTTGCATAGGAAAGGACAAGTATGGCGAGGAGATGACCAAGAACTCTAGACTTGCTGGTGTAAATGTTCAATATTATGAAGATGAAAATACACCTACAGGAACTTGCGCTGTTTGTGTGGTTGGTGGTGAAAGGTCACTTATTGCCAACTTAGCAGCTGCAAATTGCTACAAGGTTGATCATTTGAAGAAACCCGAAAATTGGGCATTAGTTGAAAAGGCCAAGTATTTCTATATTGCTGGCTTTTTCCTCACCGTATCCCCCGAATCCATACAATTAGTTGGCGAACATGCAGCTGCAAAGAACAAGGTCTTCACGATGAACCTTTCTGCACCCTTTATCTGTGAGTTCTTCAAGGATGTACAGGATAAAGCTCTTGCGTATGTTGACTATGTTTTTGGAAATGAGACTGAAGCAAGAACATATTCTAAAGTGCACGGCTGGGAGACCGACAATGTCGAGGAGATAGCTATAAAGATATCTGAGTTGCCAAAAGCAAATGAAGCTCGTAAAAGGATCACTGTTATCACACAGGGTGCCGATCCCGTGTGTGTTGCTAAAGATGGGAAAGTGACATTGTACCCTGTAATTCTATTGCCTAAAGAGAAATTAGTTGATACAAATGGAGCAGGAGATGCATTTGTTGGAGGATTCCTTGCTCAATTGGTTCAGGAGAAGTCTATTGAAGAAAGTGTGAGAGCTGGGTGTTATGCAGCAAATGTTGTCATTCAAAGGTCCGGCTGCACTTACCCAGAGAAGCCTGATTTTCATTGAGCGATTCTCCCGATTTTATCATCATCATTATTATTATTACTATGAAGTTTTGATTATTGGGAGCAATTTTAGTTGCATTTTAGTAGTTACACTCTTAACTGGTTTGGGATTTTTATTAAATCCCGGATAGGGAGAAGAGGTGAAAAAGTTGCAGAGGGAAACATTACTGAAATAAGTTCATGTTATTTGAAGTCCTATCTGGCATCATTTATAATCTTTTGCTGTTTGTCATTTCAAGTGTTTGTCTTTTATGGTTACTATAGCTAATGTTGCTATATGATGATACTGAGAAAATAGCTAATGTTGCTTTTACATGTTCTATCTTATTTGAAATCCTATCAGGTTATAATGTTGCTATTTGTTATCNACA
TARGET=143,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0168
PRIMER_LEFT_PENALTY=0.012470
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=TCATTCAACAACACCACCTCTC
PRIMER_RIGHT_SEQUENCE=AATTGAATTCTGAGTGGCACCT
PRIMER_LEFT=87,22
PRIMER_RIGHT=361,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.2437
PRIMER_LEFT_1_PENALTY=0.012470
PRIMER_RIGHT_1_PENALTY=0.231280
PRIMER_LEFT_1_SEQUENCE=TCATTCAACAACACCACCTCTC
PRIMER_RIGHT_1_SEQUENCE=GTTTATCTTCAGCGAGAATGGC
PRIMER_LEFT_1=87,22
PRIMER_RIGHT_1=284,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=60.231
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.2540
PRIMER_LEFT_2_PENALTY=0.012470
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=TCATTCAACAACACCACCTCTC
PRIMER_RIGHT_2_SEQUENCE=TTACCCATACCGAGGAGAACAC
PRIMER_LEFT_2=87,22
PRIMER_RIGHT_2=192,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=106
PRIMER_PAIR_PENALTY_3=0.2872
PRIMER_LEFT_3_PENALTY=0.012470
PRIMER_RIGHT_3_PENALTY=0.274705
PRIMER_LEFT_3_SEQUENCE=TCATTCAACAACACCACCTCTC
PRIMER_RIGHT_3_SEQUENCE=ATTACCCATACCGAGGAGAACA
PRIMER_LEFT_3=87,22
PRIMER_RIGHT_3=193,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=59.725
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=0.4051
PRIMER_LEFT_4_PENALTY=0.012470
PRIMER_RIGHT_4_PENALTY=0.392624
PRIMER_LEFT_4_SEQUENCE=TCATTCAACAACACCACCTCTC
PRIMER_RIGHT_4_SEQUENCE=ACCTCCAGCAATATACTCCACG
PRIMER_LEFT_4=87,22
PRIMER_RIGHT_4=343,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=60.393
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_03332 Ps_contig_mira-and-tgicl-ass_franssen_29580
SEQUENCE=TAAGTAGTTGGTTATTCTAACGCAGAGTACGCGGGGATTGACGAGTGACTTTTATTTAAACCTACTTCACTCCAAAACTATTCTGATTCATTCAACAACACCACCTCTCTCGCCTCTCCTTTTTCTCTGCTCAGTTTTTTCGCATCCATCCATCAGTAATGGCGTTGGAAGGTGTTCTCCTCGGTATGGGTAATCCCCTTCTCGACATTTCTGCTGTCGTCGACGAAGATTTCTTGAAAAAGTTTGATATTCAGTTGAACAATGCCATTCTCGCTGAAGATAAACACAAATCTATGTACGATGAAATGTCTGCCAAATTTAACGTGGAGTATATTGCTGGAGGTGCCACTCAGAATTCAATTAAGGTTGCTCAGTGGTTGCTTCAAGTACCCGGAGCCACTAGTTACATTGGTTGCATAGGAAAGGACAAGTATGGCGAGGAGATGACCAAGAACTCTAGACTTGCTGGTGTAAATGTTCAATATTATGAAGATGAAAATACACCTACAGGAACTTGCGCTGTTTGTGTGGTTGGTGGTGAAAGGTCACTTATTGCCAACTTAGCAGCTGCAAATTGCTACAAGGTTGATCATTTGAAGAAACCCGAAAATTGGGCATTAGTTGAAAAGGCCAAGTATTTCTATATTGCTGGCTTTTTCCTCACCGTATCCCCCGAATCCATACAATTAGTTGGCGAACATGCAGCTGCAAAGAACAAGGTCTTCACGATGAACCTTTCTGCACCCTTTATCTGTGAGTTCTTCAAGGATGTACAGGATAAAGCTCTTGCGTATGTTGACTATGTTTTTGGAAATGAGACTGAAGCAAGAACATATTCTAAAGTGCACGGCTGGGAGACCGACAATGTCGAGGAGATAGCTATAAAGATATCTGAGTTGCCAAAAGCAAATGAAGCTCGTAAAAGGATCACTGTTATCACACAGGGTGCCGATCCCGTGTGTGTTGCTAAAGATGGGAAAGTGACATTGTACCCTGTAATTCTATTGCCTAAAGAGAAATTAGTTGATACAAATGGAGCAGGAGATGCATTTGTTGGAGGATTCCTTGCTCAATTGGTTCAGGAGAAGTCTATTGAAGAAAGTGTGAGAGCTGGGTGTTATGCAGCAAATGTTGTCATTCAAAGGTCCGGCTGCACTTACCCAGAGAAGCCTGATTTTCATTGAGCGATTCTCCCGATTTTATCATCATCATTATTATTATTACTATGAAGTTTTGATTATTGGGAGCAATTTTAGTTGCATTTTAGTAGTTACACTCTTAACTGGTTTGGGATTTTTATTAAATCCCGGATAGGGAGAAGAGGTGAAAAAGTTGCAGAGGGAAACATTACTGAAATAAGTTCATGTTATTTGAAGTCCTATCTGGCATCATTTATAATCTTTTGCTGTTTGTCATTTCAAGTGTTTGTCTTTTATGGTTACTATAGCTAATGTTGCTATATGATGATACTGAGAAAATAGCTAATGTTGCTTTTACATGTTCTATCTTATTTGAAATCCTATCAGGTTATAATGTTGCTATTTGTTATCNACA
TARGET=1211,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1253
PRIMER_LEFT_PENALTY=0.047267
PRIMER_RIGHT_PENALTY=0.078029
PRIMER_LEFT_SEQUENCE=GATTTTCATTGAGCGATTCTCC
PRIMER_RIGHT_SEQUENCE=CCTCTGCAACTTTTTCACCTCT
PRIMER_LEFT=1172,22
PRIMER_RIGHT=1340,22
PRIMER_LEFT_TM=60.047
PRIMER_RIGHT_TM=59.922
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=0.1455
PRIMER_LEFT_1_PENALTY=0.067430
PRIMER_RIGHT_1_PENALTY=0.078029
PRIMER_LEFT_1_SEQUENCE=GGTGTTATGCAGCAAATGTTGT
PRIMER_RIGHT_1_SEQUENCE=CCTCTGCAACTTTTTCACCTCT
PRIMER_LEFT_1=1113,22
PRIMER_RIGHT_1=1340,22
PRIMER_LEFT_1_TM=59.933
PRIMER_RIGHT_1_TM=59.922
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=0.1554
PRIMER_LEFT_2_PENALTY=0.077340
PRIMER_RIGHT_2_PENALTY=0.078029
PRIMER_LEFT_2_SEQUENCE=GAAGAAAGTGTGAGAGCTGGGT
PRIMER_RIGHT_2_SEQUENCE=CCTCTGCAACTTTTTCACCTCT
PRIMER_LEFT_2=1094,22
PRIMER_RIGHT_2=1340,22
PRIMER_LEFT_2_TM=59.923
PRIMER_RIGHT_2_TM=59.922
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.2156
PRIMER_LEFT_3_PENALTY=0.137550
PRIMER_RIGHT_3_PENALTY=0.078029
PRIMER_LEFT_3_SEQUENCE=AGAAGCCTGATTTTCATTGAGC
PRIMER_RIGHT_3_SEQUENCE=CCTCTGCAACTTTTTCACCTCT
PRIMER_LEFT_3=1164,22
PRIMER_RIGHT_3=1340,22
PRIMER_LEFT_3_TM=59.862
PRIMER_RIGHT_3_TM=59.922
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=0.3007
PRIMER_LEFT_4_PENALTY=0.047267
PRIMER_RIGHT_4_PENALTY=0.253482
PRIMER_LEFT_4_SEQUENCE=GATTTTCATTGAGCGATTCTCC
PRIMER_RIGHT_4_SEQUENCE=TCAGTAATGTTTCCCTCTGCAA
PRIMER_LEFT_4=1172,22
PRIMER_RIGHT_4=1353,22
PRIMER_LEFT_4_TM=60.047
PRIMER_RIGHT_4_TM=59.747
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_03333 Ps_contig_mira-and-tgicl-ass_franssen_29590
SEQUENCE=GGTTCAAAACAAGGGCGCTTTGTAACACCTACTAAACCCCCTTCCATTTTGCTTCAAANAACGCTATCCATATTTCCTCTCACACTCACCATTGCTCAATCCACAACAATGGCTACAACCCCTTCGCTTCTACACTCTTCCGGCTCCTCTTTTCTCCCTCAATTTCGAACTTTTCCAACCCGATTTTCTTCTTCTTCCTTGTTTTCTCATGGAAACAACCGGCACGGAAGTGGAGTGGTGTCCGTGAAGGCCACGGCTTCTGGGGCTGTGTTGGTGGAGAAGTCTGAAGCAGAGAAGGTTTATAGACTCAAAACAACTTACAATGAGAAAATTGTTCCTTTGCTCATGGAAGAGTTCTCTTACACCAACATTCATCAGGTGCCTAAAGTTGATCAGATTGTGGTAAACTGTGGTATTGGAGAAGCTGCACAGAATGCAAAAGGTTTGGATGCAGCAATTAATGATCTAGCATTGATCACTGGGCAGAGGCCTGTTAAGACCCGGGCGAGGATTTCTGTTGCCACCTTCAAGATCAGGGAAGGTCAACCACTTGGAATTGCTGTGACACTCCGAGGAAAAATAATGTACTCATTTCTAGACCGAGTTATCAACTTAGGACTTCCTAGGACAAGAGATTTTCAAGGTTTGAATCTAAGCAGCTTTGATGGGAATGGAAATTTCAACATTGGTATCAAGGACCAAACAGTGTTCCCCGAGCTGAAATCTGGTTTAGGTACACCTAGAGGAATGGATGTATGCATCAAAACAACTGCTAAAACTGATCAAGAAGGACAAAAATTACTAGCTCTCATGGGTATGCCCTTCAGAGAGGGAGTTGAAGTTACTCAAATAGTTCGTAAAAAGAAGTTGAGGTCTCATCATTTTGATGCAAAATCAAAGGGTAGAGGAGATAGAGCAAAGAAGTAAAATCATTTTTATGAATGCAAAATCAGAGGAATAATGATCTAAGGTTGTAACTAGAAGATACACTATGTTGTAACCCTTGTTTCATTCAGATGTAATGTAAGATTATTGATTCTTTTTGTAAGAGATATGAATGTTTTCATATTGTCGTGTTATTACAAGGTTGTATTATGGTCATGCAGATCATGCTTGCTNACTAAGCTCTAGAGGTCGCAATTTGATCTATTAATTAATCTATATATTAAAT
TARGET=186,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3915
PRIMER_LEFT_PENALTY=0.148509
PRIMER_RIGHT_PENALTY=0.243031
PRIMER_LEFT_SEQUENCE=TTTCCTCTCACACTCACCATTG
PRIMER_RIGHT_SEQUENCE=ACTCTTCCATGAGCAAAGGAAC
PRIMER_LEFT=72,22
PRIMER_RIGHT=354,22
PRIMER_LEFT_TM=60.149
PRIMER_RIGHT_TM=59.757
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.4831
PRIMER_LEFT_1_PENALTY=0.148509
PRIMER_RIGHT_1_PENALTY=0.334586
PRIMER_LEFT_1_SEQUENCE=TTTCCTCTCACACTCACCATTG
PRIMER_RIGHT_1_SEQUENCE=TCAGACTTCTCCACCAACACAG
PRIMER_LEFT_1=72,22
PRIMER_RIGHT_1=286,22
PRIMER_LEFT_1_TM=60.149
PRIMER_RIGHT_1_TM=60.335
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=215
PRIMER_PAIR_PENALTY_2=0.5048
PRIMER_LEFT_2_PENALTY=0.501224
PRIMER_RIGHT_2_PENALTY=0.003589
PRIMER_LEFT_2_SEQUENCE=CTCAATCCACAACAATGGCTAC
PRIMER_RIGHT_2_SEQUENCE=TTTAGGCACCTGATGAATGTTG
PRIMER_LEFT_2=94,22
PRIMER_RIGHT_2=386,22
PRIMER_LEFT_2_TM=59.499
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.5370
PRIMER_LEFT_3_PENALTY=0.533373
PRIMER_RIGHT_3_PENALTY=0.003589
PRIMER_LEFT_3_SEQUENCE=TACAACCCCTTCGCTTCTACAC
PRIMER_RIGHT_3_SEQUENCE=TTTAGGCACCTGATGAATGTTG
PRIMER_LEFT_3=113,22
PRIMER_RIGHT_3=386,22
PRIMER_LEFT_3_TM=60.533
PRIMER_RIGHT_3_TM=59.996
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.6157
PRIMER_LEFT_4_PENALTY=0.501224
PRIMER_RIGHT_4_PENALTY=0.114470
PRIMER_LEFT_4_SEQUENCE=CTCAATCCACAACAATGGCTAC
PRIMER_RIGHT_4_SEQUENCE=AGAACTCTTCCATGAGCAAAGG
PRIMER_LEFT_4=94,22
PRIMER_RIGHT_4=357,22
PRIMER_LEFT_4_TM=59.499
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_03334 Ps_contig_mira-and-tgicl-ass_franssen_29591
SEQUENCE=TCGGGGGTGATATATATTGTGACGTCTCTTACTAGTCAAGCCTAGTCAAGCCCTTTGGGGCTATATATAAGTGTTTTACAATCATCNCGGGATCACACAAACACACATAACACATTAAGTGAAGTGAGTCATATTAAGTTTTTATATTCATCACTACTTAAGAAGCCATGGAGAAGAAATCACTAGCTTGCTTGTCCTTCCTCCTCCTCGTTCTCTTTGTTGCACAAGAAATAGTGGTGAGTGAAGCAAACACATGTGAGAATTTGGCTGGTTCATATAAGGGAGTATGCTTCGGTGGATGTGACCGTCACTGTAGAACACAAGAGGGCGCAATTAGCGGCAGATGCAGGGATGACTTTCGCTGTTGGTGCACTAAAAACTGTTAAATCCCTTTTCTCCAACACCAACAACACCATTATAGAATACTATAATATAAATAAATAAACAAGTGTTGTTTCTGAATTCTATGTGTGTACTCAATATCGTGTATAACGTCTTTGTTATGCACTTTTATCATATCATATGGAAATAAAAAGTAATCAATCATTTCCTTTCC
TARGET=433,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03335 Ps_contig_mira-and-tgicl-ass_franssen_29593
SEQUENCE=CGANTGTTNNNCTATAAGTAGCATTTATAAATAAATAAATAAAGTATAAACTAACTGATGCCATCATTTCATCAAAATGTCCACCATAAGCATACCTGGATATCCAAAAAAACAGTCCCCAATTATTCTGTTATAGATATGCATAATCCCGCAATATGGATCATTACCAAAACTAACAGAAAAACAGCACCCTTCTTCACAACAAAATAGTTTAACTACCCATCACACTTTGTATATTTTACCTAATCACTGTCTGAATGAGAAACAAGTAATTGGAGTGTGATCCAACAACCCTGGAAAAAAACTCACTAAGAGACCTTTGATTCTGCTCAGACATAGTATCTCATGCAGGCATTGTGGTCGGTTTTCAGAAGTCTGACTATAAATTTATACTTTCTTGATAATCTGAACAACGTCTTCGTCTTCAAGTTCATGTTCCTTGCCGACTCTTTGAGGCTTGTGTTTTGCACTTGAACCCCAGACCAGAGCATACTTAAACTGTTTAAGCATGTCCTTGTGAATGCGGTCGCAGAAGTCTTCGACTGTCTTCTTCTTAGACGACAATATTACAGGGTCTTCATAATCAGGATTCATACCTTTCGGTTTAGTATAAATACGAGTTAAACAGAGATACTCCCAAACTTTATCCAGAAGCCCATCAAGATTCCACTCCAGGTGCGCACTGACAGGGCAGTAATGAGGAAGCTTATCCAATATCTCCAGTTCCTCAAGTGTGATCTGATCAATCTTGTTCACAACATAGATGCAGGGTGTATAGATTCTACTGCCCTCAATGACATCTATAAGGTCATCAGCAGTAGCATCATACCTTAGAGTAATATCAGCATTATGAATTCTGTACTCGCTACAGATAGCCTTCACGGTATCGAGATCCAGATGGGTGTTTGTAGCAGTTGAGGTGAAATTAATTCCACCCTTCTCTTTCCTCCTAAAAGTCAGGTTTGGGGGTTCTTTGTTTAACCTTATACCAAATCCTTCTAGCTCTTTTTCAATAAGACGCTTATGAGTTATTGGTTTTATTGCATCGAGAACAATTAAAATGCAATTACATGTCCTTGCAGTACTAATAACCTGCCTTCCTCTACCCTTTCCATCCTTGGCACCCTCAATAATTCCAGGAAGATCCAGCAACTGAATTTTAGCTCCACGGTATGTGATCACACCTGGGATGCATGTCAAGGTAGTGAACTCATAGGAAGCAACCTCTGAAAATGTCCCAGTCAATTTATTCAGCAGAGTAGACTTGCCAACCGAAGGAAAGCCCACAAGACCAACTCTTGCATCTCCACTTTTAGTAACATCAAAACCTTCACCAGCACCTCCACCTCCCTTAGTTGTAGGGGTAAGGAGCTCTCTCCGCAGTTTTGCAAGTTTAGCCTTCAGTAACCCTAAATGATGAGCAGTAGCCTTGTTCTTTTGAGTCTTTGCCATCTCATCTTCAATATCCTTGATTTTCTGCATGACCGTCGCCATTGTTGAAGGAAGATAAGCCCGGAAGCAAAGGAAAAGAGATTGAGTTTAGTTGTGGAGAGAAGTGAAGTGAGATTGGACTTGGATGACTCTGCGGTAGTGAATTGTTACAGGCGTTGAGCGTGGTGTTGCACCGGAGAAGAGAATGGGGACGAAGGTGCTAGAACAAATATGCAGAAATGGTTATGGTCGGACTT
TARGET=27,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03336 Ps_contig_mira-and-tgicl-ass_franssen_29596
SEQUENCE=TTTCTTTTAACAGTTGAGTGAAGCTACCGGATAATTCAAAGGCAAAAAGGAGCGAATAAGAATTAAAATACATAGTAGTAAACCACAGGCAGAAACAACAACATCCCCTAGGGTTAAAAGATCATTTGCCATATAACATGTATTTGATTAACCCATGCAGGAATACGCAAGAGAGTCCAGAAAGACGTTGTCTTGGTCCAAGCAGGAATTAAAGGACTGCTTGTATTAAATAACTGTGCCTTATGAACTCCAAGTATCACAACCCTCTCAAATCTCAACTTCCTGCTGTACTTGGTACAGGCTCTGATTTTGAGTCTTCTTCCCTTTCATGTTCTTTGTTGAGCATCTTGTTGTTAATCAAGTTGTGGAGTGCAATTACACTTCTGATGAGGGACGATAGGTATATTACTAGCATCATATCATTAGTTTTCACTGCAAAAGCCTTGATCAAATCAGTGACATTAAGATTTGGCAGCAGGTTGAATACGTCCTGCAAATGGTAAAGGATCTCATGGTTTAAGGGAAGCTTTCCATCAATGACAAGGTCAAGGTAACTTCTTATCTCTTTGAGTCTTGCATCCAAACCCTTCAAGGCTGTGAGTTTTGCACTTACCTCTGTTGCAAGGGTGCTAATGGTTGTATCCTTTACATCTCTAAGCAAGTGCTCAACTCCTATTTCCTCAACTTCATGAGCAGCAATTTCAGATTGCACATGCACAAAGACCTTTTGGCTTTTCTGAGTGGCATTCTCTTTAACCTCTTCAACAGCATAGTAAGCTTTAGTAGGAATTCCTAGCTCCTTAGGTTCGACATCAATTATAACCAAAACAGGATTTGGAACATAGTCATTGAATAAACCATGAATGTCAAGGTCATTTTCTCGCAGTTTTGGTCCAGTACTATACCACCCTACAACGTGCTCCTTAGCATTGATTCTCTTAAACATGGAGAACATTGATTCATGATAGTTATGATCGAGAAACCAAATGCTAGGATCCTTTTCGTCTTCTTCAAAAGGCACGGCGTAGCTGTTGGTGATATCAACGGTGCCTTTGAAGGAGGAACCGAGCAAGACGCCGACGACGCGTTTGCGAGTGTCTTTGGCGACTCTGTTGTAGTTATCGACTATGCTAAGCAAAACTAAAGGGTGAACTACGACTTTTTCTATGGCTCTCCATGAAATTTGTTGCGTTTTTATCACATCCATTGTTGTTGTTGTTGTGATTTTTCAGTGCTTGAAACCCTAATTTTTGTTCTCTAGGAATTGAGGAATGAAAAATAGAAATTCTTTAGTGTGTTTGTTTGTTTCTTCTTCCTGATTCCCATCTCACCTAAACCCTCTCT
TARGET=1207,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1088
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=GATCGAGAAACCAAATGCTAGG
PRIMER_RIGHT_SEQUENCE=GGGTTTCAAGCACTGAAAAATC
PRIMER_LEFT=972,22
PRIMER_RIGHT=1243,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.1570
PRIMER_LEFT_1_PENALTY=0.143555
PRIMER_RIGHT_1_PENALTY=0.013401
PRIMER_LEFT_1_SEQUENCE=TGCTAGGATCCTTTTCGTCTTC
PRIMER_RIGHT_1_SEQUENCE=GGGTTTCAAGCACTGAAAAATC
PRIMER_LEFT_1=987,22
PRIMER_RIGHT_1=1243,22
PRIMER_LEFT_1_TM=59.856
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.3096
PRIMER_LEFT_2_PENALTY=0.296177
PRIMER_RIGHT_2_PENALTY=0.013401
PRIMER_LEFT_2_SEQUENCE=GGATCCTTTTCGTCTTCTTCAA
PRIMER_RIGHT_2_SEQUENCE=GGGTTTCAAGCACTGAAAAATC
PRIMER_LEFT_2=992,22
PRIMER_RIGHT_2=1243,22
PRIMER_LEFT_2_TM=59.704
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=0.3833
PRIMER_LEFT_3_PENALTY=0.369874
PRIMER_RIGHT_3_PENALTY=0.013401
PRIMER_LEFT_3_SEQUENCE=CAAATGCTAGGATCCTTTTCGT
PRIMER_RIGHT_3_SEQUENCE=GGGTTTCAAGCACTGAAAAATC
PRIMER_LEFT_3=983,22
PRIMER_RIGHT_3=1243,22
PRIMER_LEFT_3_TM=59.630
PRIMER_RIGHT_3_TM=59.987
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.6064
PRIMER_LEFT_4_PENALTY=0.593025
PRIMER_RIGHT_4_PENALTY=0.013401
PRIMER_LEFT_4_SEQUENCE=CCTTTTCGTCTTCTTCAAAAGG
PRIMER_RIGHT_4_SEQUENCE=GGGTTTCAAGCACTGAAAAATC
PRIMER_LEFT_4=996,22
PRIMER_RIGHT_4=1243,22
PRIMER_LEFT_4_TM=59.407
PRIMER_RIGHT_4_TM=59.987
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_03337 Ps_contig_mira-and-tgicl-ass_franssen_29596
SEQUENCE=TTTCTTTTAACAGTTGAGTGAAGCTACCGGATAATTCAAAGGCAAAAAGGAGCGAATAAGAATTAAAATACATAGTAGTAAACCACAGGCAGAAACAACAACATCCCCTAGGGTTAAAAGATCATTTGCCATATAACATGTATTTGATTAACCCATGCAGGAATACGCAAGAGAGTCCAGAAAGACGTTGTCTTGGTCCAAGCAGGAATTAAAGGACTGCTTGTATTAAATAACTGTGCCTTATGAACTCCAAGTATCACAACCCTCTCAAATCTCAACTTCCTGCTGTACTTGGTACAGGCTCTGATTTTGAGTCTTCTTCCCTTTCATGTTCTTTGTTGAGCATCTTGTTGTTAATCAAGTTGTGGAGTGCAATTACACTTCTGATGAGGGACGATAGGTATATTACTAGCATCATATCATTAGTTTTCACTGCAAAAGCCTTGATCAAATCAGTGACATTAAGATTTGGCAGCAGGTTGAATACGTCCTGCAAATGGTAAAGGATCTCATGGTTTAAGGGAAGCTTTCCATCAATGACAAGGTCAAGGTAACTTCTTATCTCTTTGAGTCTTGCATCCAAACCCTTCAAGGCTGTGAGTTTTGCACTTACCTCTGTTGCAAGGGTGCTAATGGTTGTATCCTTTACATCTCTAAGCAAGTGCTCAACTCCTATTTCCTCAACTTCATGAGCAGCAATTTCAGATTGCACATGCACAAAGACCTTTTGGCTTTTCTGAGTGGCATTCTCTTTAACCTCTTCAACAGCATAGTAAGCTTTAGTAGGAATTCCTAGCTCCTTAGGTTCGACATCAATTATAACCAAAACAGGATTTGGAACATAGTCATTGAATAAACCATGAATGTCAAGGTCATTTTCTCGCAGTTTTGGTCCAGTACTATACCACCCTACAACGTGCTCCTTAGCATTGATTCTCTTAAACATGGAGAACATTGATTCATGATAGTTATGATCGAGAAACCAAATGCTAGGATCCTTTTCGTCTTCTTCAAAAGGCACGGCGTAGCTGTTGGTGATATCAACGGTGCCTTTGAAGGAGGAACCGAGCAAGACGCCGACGACGCGTTTGCGAGTGTCTTTGGCGACTCTGTTGTAGTTATCGACTATGCTAAGCAAAACTAAAGGGTGAACTACGACTTTTTCTATGGCTCTCCATGAAATTTGTTGCGTTTTTATCACATCCATTGTTGTTGTTGTTGTGATTTTTCAGTGCTTGAAACCCTAATTTTTGTTCTCTAGGAATTGAGGAATGAAAAATAGAAATTCTTTAGTGTGTTTGTTTGTTTCTTCTTCCTGATTCCCATCTCACCTAAACCCTCTCT
TARGET=1296,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0886
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=0.075241
PRIMER_LEFT_SEQUENCE=GATTTTTCAGTGCTTGAAACCC
PRIMER_RIGHT_SEQUENCE=AGGGTTTAGGTGAGATGGGAAT
PRIMER_LEFT=1222,22
PRIMER_RIGHT=1339,22
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=118
PRIMER_PAIR_PENALTY_1=0.4563
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=0.442898
PRIMER_LEFT_1_SEQUENCE=GATTTTTCAGTGCTTGAAACCC
PRIMER_RIGHT_1_SEQUENCE=GTGAGATGGGAATCAGGAAGAA
PRIMER_LEFT_1=1222,22
PRIMER_RIGHT_1=1330,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=60.443
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.5828
PRIMER_LEFT_2_PENALTY=0.013401
PRIMER_RIGHT_2_PENALTY=0.569350
PRIMER_LEFT_2_SEQUENCE=GATTTTTCAGTGCTTGAAACCC
PRIMER_RIGHT_2_SEQUENCE=TGAGATGGGAATCAGGAAGAAG
PRIMER_LEFT_2=1222,22
PRIMER_RIGHT_2=1329,22
PRIMER_LEFT_2_TM=59.987
PRIMER_RIGHT_2_TM=60.569
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.8066
PRIMER_LEFT_3_PENALTY=0.731389
PRIMER_RIGHT_3_PENALTY=0.075241
PRIMER_LEFT_3_SEQUENCE=CGACTTTTTCTATGGCTCTCCA
PRIMER_RIGHT_3_SEQUENCE=AGGGTTTAGGTGAGATGGGAAT
PRIMER_LEFT_3=1155,22
PRIMER_RIGHT_3=1339,22
PRIMER_LEFT_3_TM=60.731
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=0.8299
PRIMER_LEFT_4_PENALTY=0.013401
PRIMER_RIGHT_4_PENALTY=0.816516
PRIMER_LEFT_4_SEQUENCE=GATTTTTCAGTGCTTGAAACCC
PRIMER_RIGHT_4_SEQUENCE=TTAGGTGAGATGGGAATCAGGA
PRIMER_LEFT_4=1222,22
PRIMER_RIGHT_4=1334,22
PRIMER_LEFT_4_TM=59.987
PRIMER_RIGHT_4_TM=60.817
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=113
=
PRIMER_SEQUENCE_ID=SSR_03338 Ps_contig_mira-and-tgicl-ass_franssen_29598
SEQUENCE=GTCGGGGAAAGAAAGAAAAGAAAAGAAAAAACAAAACTAGAGAGAAGTGTGTAGAGAAAGGGGTAGAAACTAAAATTGTATGATAATACAATTTTATTTCACACATTCTCTGTTGCATTATCACAAACACCTCGAACTCTATCTATCTCACTCTGCAACTAGCAATGGCTTCACAAACTCTCGTCTCACCTTCACCTCTCTCTTCTCACTCTCTTCTCCGAACATCTTTCTCCGGCGTCTCCGTCAAGCTCGCTCCCCAATTCTCAACCCTTGCAACTTCCAATTTCAAACCTCTCACCGTAGTTGCGGCTGCCAAGAAAGCCGTCGCTGTCCTTAAGGGCACATCCGCCGTCGAAGGTGTCGTCACTCTCACTCAAGACGATGAAGGTCCAACAACAGTTAATGTTCGTATCACTGGCCTTACTCCAGGGCTTCATGGTTTTCACCTACATGAGTATGGTGATACCACAAATGGGTGTATCTCAACAGGACCACATTTTAATCCCAACAAGTTGACACATGGTGCTCCTGAAGATGAAATCCGTCATGCGGGTGACCTGGGAAACATAGTTGCTAATGCTGAAGGAGTTGCAGAGGCGACAATCGTGGACAATCAGATACCACTCACTGGCCCCAATTCAGTCGTTGGGAGAGCCTTAGTGGTTCACGAGCTTCAAGATGACCTTGGAAAGGGTGGACATGAACTTAGTTTGAGCACTGGAAATGCTGGTGGAAGATTAGCTTGTGGTGTGGTTGGCTTGACTCCAGTATAAATGCTTCAAATGCTTTTGCACCAGCCTTGTTATTTTAACTGATGTTTGATTTCTTCAGTTATCCTTGTTTTATGAAGCTTTAATATTAATCACCTTTCAGAAATTAATCACCGCCATGAATGAAGCTTTACTGTTATTTGTTTTCTTCAATTAGCAATGTAAAGATTTTAATGTGTGAAAACATGACAGTCCTCTACTAGATAGTTTCATCATGCAGTCAGGTGTGTTTCTTCCCTAATAAAGTTTAATTTCTGATGTAGTTCTTGTTTCCTGCTTGTAAGAGAACCTATGTTGATATTCTGTGTTATTTGTGTTCCTAGTTAGNCTAAT
TARGET=7,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03339 Ps_contig_mira-and-tgicl-ass_franssen_29598
SEQUENCE=GTCGGGGAAAGAAAGAAAAGAAAAGAAAAAACAAAACTAGAGAGAAGTGTGTAGAGAAAGGGGTAGAAACTAAAATTGTATGATAATACAATTTTATTTCACACATTCTCTGTTGCATTATCACAAACACCTCGAACTCTATCTATCTCACTCTGCAACTAGCAATGGCTTCACAAACTCTCGTCTCACCTTCACCTCTCTCTTCTCACTCTCTTCTCCGAACATCTTTCTCCGGCGTCTCCGTCAAGCTCGCTCCCCAATTCTCAACCCTTGCAACTTCCAATTTCAAACCTCTCACCGTAGTTGCGGCTGCCAAGAAAGCCGTCGCTGTCCTTAAGGGCACATCCGCCGTCGAAGGTGTCGTCACTCTCACTCAAGACGATGAAGGTCCAACAACAGTTAATGTTCGTATCACTGGCCTTACTCCAGGGCTTCATGGTTTTCACCTACATGAGTATGGTGATACCACAAATGGGTGTATCTCAACAGGACCACATTTTAATCCCAACAAGTTGACACATGGTGCTCCTGAAGATGAAATCCGTCATGCGGGTGACCTGGGAAACATAGTTGCTAATGCTGAAGGAGTTGCAGAGGCGACAATCGTGGACAATCAGATACCACTCACTGGCCCCAATTCAGTCGTTGGGAGAGCCTTAGTGGTTCACGAGCTTCAAGATGACCTTGGAAAGGGTGGACATGAACTTAGTTTGAGCACTGGAAATGCTGGTGGAAGATTAGCTTGTGGTGTGGTTGGCTTGACTCCAGTATAAATGCTTCAAATGCTTTTGCACCAGCCTTGTTATTTTAACTGATGTTTGATTTCTTCAGTTATCCTTGTTTTATGAAGCTTTAATATTAATCACCTTTCAGAAATTAATCACCGCCATGAATGAAGCTTTACTGTTATTTGTTTTCTTCAATTAGCAATGTAAAGATTTTAATGTGTGAAAACATGACAGTCCTCTACTAGATAGTTTCATCATGCAGTCAGGTGTGTTTCTTCCCTAATAAAGTTTAATTTCTGATGTAGTTCTTGTTTCCTGCTTGTAAGAGAACCTATGTTGATATTCTGTGTTATTTGTGTTCCTAGTTAGNCTAAT
TARGET=12,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03340 Ps_contig_mira-and-tgicl-ass_franssen_29606
SEQUENCE=GTCGCGGGGATCTCATCAAACAAAATTCTCAAAACCAAAATGGGAAAAACCAAGTCCCTTTCCTTTTCATTCTTAGCAATAACATGTGCTTTTGTGCTTCACTTTCAATACATAGTTCTTGCTCAAGAACATGTGGCATCTTCAGAAAGTGAAGCACTAGGCCTAACACCAGAAACTAAACGAGAAACACTAGAAATCATCATCGGCGGAGGAGGAGGAGGCGCCTATTCTCCCGCTCCTTCCCCGGAAAATAACTGCCCTCCACCTCCACCTCCGCCGTGTCCCCCCACCCGATTAGAAAAAGCCCGTGCCGCACTTCTCAAATTCAAATCACTTATCAATGACCCCAAAGGGTACACTAATAGCTGGAACAATAATACAGAAACTTGCAACTTCAAGGGAATCCTTTGTGGAACATTCCCAGTAGATAACCAACAAGCAGTTGCTGGATTAGACTTCAACCAAGCAAAATTCTCAAGCAAAGACTGTACCAATATACCACTCACAGGCTTCACTGGTATATTAGACAAACTAGAAGAATTAACATTCTTCCATGTCAATTCTAACAATTTCTCAAACCAAATCCCAGAAGCAATCACTAAATACCCTTTTTTCTACGAACTTGACCTCAGTAACAACAAACTTTCCGGCCCGTTTCCGAAGGAAGTTCTTCTATCTACAAAACTAGTTTTCCTAGACCTCAGGTTCAACTTCCTCACCGGGTCTGTTCCATCGCAGTTATTCGAGAAAGATCTCGACGTAATCTTCATCAATAACAACCAGTTTAGTCAATGTTTACCTGCCAATTTCGGTTCTACTCCTGCAAGGTATCTAACTTTCGCACACAATCAATTCGTTGGCGAAATTCCTAGAAGTATCGGTAACGCTGCGAAAACTTTAACTGAAGTTCTTTTCTTACAGAATAAATTTGAAGGTTGTTTACCTTATGAAATAGGCTATTTGAAAAAAGCTACTGTTTTTGATGTTAGTGAAAATCTGTTAACGGGTCCTATTCCTGCTTCGTTTGGTTGTTTAGAGAAGATTCAGTTTCTGAATCTAGCACATAACAAATTCTACGGTACTGTTCCTGAGAGTGTTTGTGTTTTACCTGGGATACGTAATAATGGGAATTTGTCTCTGGCGGATAATTATTTTACTGCTCTTGAACCATCGTGTTGGAGTTTGTTGAAGTCGAAGATATTGGATGTTAGTAGGAATTGTATTCCTGGACTTCCGAATCAAAAATCGAAGCAAGAGTGTTATGCATTTCAATGTAAGGTGAAACCTTGTTCGAATCCGCCGAGTCTGAAATACGTGCCTTGTAAAACACATTGGGGGCAGAAACAGAAACAGAACACTGCCTCGGAAATGGCTACTGAGCCTGTTACTTATAAGTCTCTCAAACCACATCGGTTGAGAATGTGAGTGTTCGCCATGTAGCGAAACACTACAGATCAAAGGTGTGTCAAGTGTTATCGACACAGACACATCAATTTCTTGTGCTTATGAATTATCATTACTATTGTCTATGTCTTATTGTCAATATTTTATATGTAAAATATGTGTCGGTGTTATTAGTTCATAAGGTGCTAGTGCCATGGTTTGTTGAGCTTCAAAATAATGCTAATTTGAAGATATTTATTTTGATTCTATGGAACTATTATGTTAATTGTGTGCTGTCTTGTTCATGAATAAATATTTGTTATCTC
TARGET=208,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1727
PRIMER_LEFT_PENALTY=0.117817
PRIMER_RIGHT_PENALTY=0.054900
PRIMER_LEFT_SEQUENCE=GAACATGTGGCATCTTCAGAAA
PRIMER_RIGHT_SEQUENCE=GTACCCTTTGGGGTCATTGATA
PRIMER_LEFT=126,22
PRIMER_RIGHT=356,22
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.945
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.2069
PRIMER_LEFT_1_PENALTY=0.151991
PRIMER_RIGHT_1_PENALTY=0.054900
PRIMER_LEFT_1_SEQUENCE=ACATGTGCTTTTGTGCTTCACT
PRIMER_RIGHT_1_SEQUENCE=GTACCCTTTGGGGTCATTGATA
PRIMER_LEFT_1=81,22
PRIMER_RIGHT_1=356,22
PRIMER_LEFT_1_TM=59.848
PRIMER_RIGHT_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.2472
PRIMER_LEFT_2_PENALTY=0.192310
PRIMER_RIGHT_2_PENALTY=0.054900
PRIMER_LEFT_2_SEQUENCE=GTGAAGCACTAGGCCTAACACC
PRIMER_RIGHT_2_SEQUENCE=GTACCCTTTGGGGTCATTGATA
PRIMER_LEFT_2=148,22
PRIMER_RIGHT_2=356,22
PRIMER_LEFT_2_TM=60.192
PRIMER_RIGHT_2_TM=59.945
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.2736
PRIMER_LEFT_3_PENALTY=0.218662
PRIMER_RIGHT_3_PENALTY=0.054900
PRIMER_LEFT_3_SEQUENCE=AACATGTGCTTTTGTGCTTCAC
PRIMER_RIGHT_3_SEQUENCE=GTACCCTTTGGGGTCATTGATA
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=356,22
PRIMER_LEFT_3_TM=60.219
PRIMER_RIGHT_3_TM=59.945
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.3146
PRIMER_LEFT_4_PENALTY=0.259704
PRIMER_RIGHT_4_PENALTY=0.054900
PRIMER_LEFT_4_SEQUENCE=ATGTGGCATCTTCAGAAAGTGA
PRIMER_RIGHT_4_SEQUENCE=GTACCCTTTGGGGTCATTGATA
PRIMER_LEFT_4=130,22
PRIMER_RIGHT_4=356,22
PRIMER_LEFT_4_TM=59.740
PRIMER_RIGHT_4_TM=59.945
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_03341 Ps_contig_mira-and-tgicl-ass_franssen_29616
SEQUENCE=TATNAACGCAGAGTACNCGGGGATCGCAGATTGAACGGGATATAGTATAATATAAACCATCGATCATTATGCAGCCTTATCGTTAACACTATTTCTCTCCGTTTTTCTGAAATTACCTTTTCACAACAATGAATTCATCATCGCTACTTCGTCCTTCTCCTCCTCCTCTTCTTTTTCATCGCAACCGTTTCGTTATTTCTTCTTCATCTAGAAACCAATCTTACATCCCTAAACTCGAACCTTTTAGCCGATCCAAAATCGACAGAGTCGCTAAAAGCCTCCCTATCATTGAAAAATCTGAAAAAGATCTCTCCGATTATTGTTCTACACTTGAAGGTGATGAATGTTATAGTTGCTGGCAGGCTTATTTTGAGCTCAAAGATCTTCAAAAGGAGTCACCGAGAGGAGATATAGAGAGGTTAATTATTCAGATTGGTGGTGTAAAATCCTTGATAGGATGTTTACATGGAATTGCTGCAATTCATAAATCGAAGAAGAATGGATCAAATTTGGGCATGGAAATAGAATCAGAAGATGAACAAAATCGTTGTCCTAGACCAGATGGATTACCAAAATCTGCTGAGGAAATGATGGAAGAAGAGGAAGCGAAAATGCCTGATTCGTCGTATACTAAGCTTTATAGATCCATGGGAAAGTCACCTGCATGGTATTCTGAAGTACCAGATCATGAAACAGATTGATGATTAGTGCAATTTAACCTTGTTTATTTTTTTTTTGTTTGTAGAGAATGATTAATCATGTGAATGTGAATATGTTAGTAGTATGCTAGTTATGCTCCTATATTTGTGAGGTTTTTGTTTTTGTTTACTTTGGTTATTGCTTTTCATAAACCATGATGTCATGAAATTTCAGGAAGTAATGTATATAACATGTATCTTGTTTTTCACAATTTTAAAGTTTTGATCTAATGGAATGAAGTTTGATATTTAAATTTTTCGG
TARGET=157,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1298
PRIMER_LEFT_PENALTY=0.081670
PRIMER_RIGHT_PENALTY=0.048131
PRIMER_LEFT_SEQUENCE=CATCGATCATTATGCAGCCTTA
PRIMER_RIGHT_SEQUENCE=GCTTTTAGCGACTCTGTCGATT
PRIMER_LEFT=57,22
PRIMER_RIGHT=277,22
PRIMER_LEFT_TM=60.082
PRIMER_RIGHT_TM=60.048
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=8.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.2785
PRIMER_LEFT_1_PENALTY=0.230329
PRIMER_RIGHT_1_PENALTY=0.048131
PRIMER_LEFT_1_SEQUENCE=TGAATTCATCATCGCTACTTCG
PRIMER_RIGHT_1_SEQUENCE=GCTTTTAGCGACTCTGTCGATT
PRIMER_LEFT_1=129,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=60.230
PRIMER_RIGHT_1_TM=60.048
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.3190
PRIMER_LEFT_2_PENALTY=0.081670
PRIMER_RIGHT_2_PENALTY=0.237346
PRIMER_LEFT_2_SEQUENCE=CATCGATCATTATGCAGCCTTA
PRIMER_RIGHT_2_SEQUENCE=GATAGGGAGGCTTTTAGCGACT
PRIMER_LEFT_2=57,22
PRIMER_RIGHT_2=286,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=60.237
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.3190
PRIMER_LEFT_3_PENALTY=0.081670
PRIMER_RIGHT_3_PENALTY=0.237346
PRIMER_LEFT_3_SEQUENCE=CATCGATCATTATGCAGCCTTA
PRIMER_RIGHT_3_SEQUENCE=ATAGGGAGGCTTTTAGCGACTC
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=285,22
PRIMER_LEFT_3_TM=60.082
PRIMER_RIGHT_3_TM=60.237
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=0.3260
PRIMER_LEFT_4_PENALTY=0.081670
PRIMER_RIGHT_4_PENALTY=0.244324
PRIMER_LEFT_4_SEQUENCE=CATCGATCATTATGCAGCCTTA
PRIMER_RIGHT_4_SEQUENCE=TCGGCTAAAAGGTTCGAGTTTA
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=250,22
PRIMER_LEFT_4_TM=60.082
PRIMER_RIGHT_4_TM=60.244
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_03342 Ps_contig_mira-and-tgicl-ass_franssen_29617
SEQUENCE=CAGCAATTCCATGTAAACATCCTATCAAGGATTTTACACCACCAATCTGAATAATTAACCTCTCTATATCTCCTCTCGGTGACTCCTTTTGAAGATCTTTGAGCTCAAAATAAGCCTGCCAGCAACTATAACATTCATCACCTTCAAGTGTAGAACAATAATCGGAGAGATCTTTTTCAGATTTTTCAATGATAGGGAGGTCTTTTAGCGACTCTGTCGAATTTGGATCGGACTAAAAGGTTCGAGTTTAGGGATGTAAGATNNGGTTTCTAGATAGAAGAAAGAAATAACGGAAACGGTTGACGAATAAGGAAAAAGAAGAGGAGGAGGAGAAGGACGAAGTAGCGATGATAGTAATTCTAGTTTGGTTAGTAAGGTAAAAGTTAATTACGAAAAGGAAAACGGAGAAAGTAAAGTTAGGTTAAGTTAAACGGAAGTAACGG
TARGET=321,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0208
PRIMER_LEFT_PENALTY=0.084740
PRIMER_RIGHT_PENALTY=0.936059
PRIMER_LEFT_SEQUENCE=AGGGAGGTCTTTTAGCGACTCT
PRIMER_RIGHT_SEQUENCE=CTTTCTCCGTTTTCCTTTTCGT
PRIMER_LEFT=193,22
PRIMER_RIGHT=410,22
PRIMER_LEFT_TM=59.915
PRIMER_RIGHT_TM=60.936
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=1.0208
PRIMER_LEFT_1_PENALTY=0.084740
PRIMER_RIGHT_1_PENALTY=0.936059
PRIMER_LEFT_1_SEQUENCE=AGGGAGGTCTTTTAGCGACTCT
PRIMER_RIGHT_1_SEQUENCE=ACTTTCTCCGTTTTCCTTTTCG
PRIMER_LEFT_1=193,22
PRIMER_RIGHT_1=411,22
PRIMER_LEFT_1_TM=59.915
PRIMER_RIGHT_1_TM=60.936
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=9.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=1.1122
PRIMER_LEFT_2_PENALTY=0.176180
PRIMER_RIGHT_2_PENALTY=0.936059
PRIMER_LEFT_2_SEQUENCE=AGCCTGCCAGCAACTATAACAT
PRIMER_RIGHT_2_SEQUENCE=CTTTCTCCGTTTTCCTTTTCGT
PRIMER_LEFT_2=112,22
PRIMER_RIGHT_2=410,22
PRIMER_LEFT_2_TM=60.176
PRIMER_RIGHT_2_TM=60.936
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=1.1122
PRIMER_LEFT_3_PENALTY=0.176180
PRIMER_RIGHT_3_PENALTY=0.936059
PRIMER_LEFT_3_SEQUENCE=AGCCTGCCAGCAACTATAACAT
PRIMER_RIGHT_3_SEQUENCE=ACTTTCTCCGTTTTCCTTTTCG
PRIMER_LEFT_3=112,22
PRIMER_RIGHT_3=411,22
PRIMER_LEFT_3_TM=60.176
PRIMER_RIGHT_3_TM=60.936
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=1.1529
PRIMER_LEFT_4_PENALTY=0.216800
PRIMER_RIGHT_4_PENALTY=0.936059
PRIMER_LEFT_4_SEQUENCE=TGATAGGGAGGTCTTTTAGCGA
PRIMER_RIGHT_4_SEQUENCE=CTTTCTCCGTTTTCCTTTTCGT
PRIMER_LEFT_4=189,22
PRIMER_RIGHT_4=410,22
PRIMER_LEFT_4_TM=60.217
PRIMER_RIGHT_4_TM=60.936
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_03343 Ps_contig_mira-and-tgicl-ass_franssen_29630
SEQUENCE=CCTTAAAAATATCTCACCACTATTATATGGTATGATCCACAATTCTNTGAAATAAAATAAATTAAACATCAAGAATAAAATTGGATTTTGCACAACAAACTAGGGAATAAAATAATTCACATAAAACCATAGCAAAACAAATTAATATATTACAATGTGATGCAAAACATGAAGAACATGTTCACAAATTCTTCCCTTGGAAACTACTTCAAATTTTCATGCATTATGCACCAAAACATCTATCCTAATGGAACTCTCCATTTTTTTCAATGGTGGTGGCGGACACATTCGGCGGGGATGACGGGAAACCTAGCGGTATCGGCGCAATAATCGTAAACCATATGATTAGCCCTAACCCAAATAAGTTGATGACTTTGATGCAAAGTGAGTTCAGACAACATAGGTTCATCCCACCAAAATTTCTTATCCTCACTACTACTACATTTCTTAGCATTATCAATGGATGTAACAGGTGTTGGACATTCACAAGCATTGATTTCGAAATCTTTATAAGTAGCAACAAAAGGTGCATGAGACCAATCTGTTTTCACACGACCACCTTGTGTGGCCCAATCATCAGCATTCCATATTGAACTATAAACACCCATTGCTTGGTCTTTGGGGAAAGGGATTCCTTTGTGTTCCAAATTTGTGTGTACTCTAATTGGTGTCTCATCCACTAGAAACACTACTTGACGTTGGTTCCAAAAGATTGAGTAGGTGTGAAAATCCTTAGATGGATCAAACCATAGGTTAAGTCTTTGTTCTCTATTACCAACACCATTCACATACACATTTGTTTGAACTGAGTAAGGTTCACCAGTTGTGTTGCCCAAAAACTCAAAATCAAATTCATTATGAGTTGGTCCTTCTGATGACATATAGAAAGCTGTAACAGTTCCAGCAGAGTCACCTTCCACAAGCTTAAGCTGAACAGTCACTTTTCCAAACATATATTTGCTCTTAGATCCAAATCCAGCACCGGAATAATTGTCGAGTTTGAGTTTGAGTAAATCTCCTTCATGAACGAAATGGTCCATAGCCCAACCAGGTTGAAAAAGCTCCTCAAATTTGGAAGAAGCAACTAATGCAACAAACAAAAAACTCAGAGAAAGTCCTAAGAGACAAGACATTTTTGTCATGTTGAAAAACACAGACAAAAAAATCGATGAAGAAGAAGAAAGAGAGAGTGAGTGAGTGAAGTGAAGCTTCTACTCTGAAATTGTGTCCCCGACGTA
TARGET=1171,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0024
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.893511
PRIMER_LEFT_SEQUENCE=CCTCAAATTTGGAAGAAGCAAC
PRIMER_RIGHT_SEQUENCE=CGGGGACACAATTTCAGAGTAG
PRIMER_LEFT=1063,22
PRIMER_RIGHT=1232,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.894
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=1.1302
PRIMER_LEFT_1_PENALTY=0.236697
PRIMER_RIGHT_1_PENALTY=0.893511
PRIMER_LEFT_1_SEQUENCE=ACCGGAATAATTGTCGAGTTTG
PRIMER_RIGHT_1_SEQUENCE=CGGGGACACAATTTCAGAGTAG
PRIMER_LEFT_1=980,22
PRIMER_RIGHT_1=1232,22
PRIMER_LEFT_1_TM=60.237
PRIMER_RIGHT_1_TM=60.894
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=1.1302
PRIMER_LEFT_2_PENALTY=0.236697
PRIMER_RIGHT_2_PENALTY=0.893511
PRIMER_LEFT_2_SEQUENCE=CACCGGAATAATTGTCGAGTTT
PRIMER_RIGHT_2_SEQUENCE=CGGGGACACAATTTCAGAGTAG
PRIMER_LEFT_2=979,22
PRIMER_RIGHT_2=1232,22
PRIMER_LEFT_2_TM=60.237
PRIMER_RIGHT_2_TM=60.894
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=1.1305
PRIMER_LEFT_3_PENALTY=0.108929
PRIMER_RIGHT_3_PENALTY=1.021538
PRIMER_LEFT_3_SEQUENCE=CCTCAAATTTGGAAGAAGCAAC
PRIMER_RIGHT_3_SEQUENCE=CGGGGACACAATTTCAGAGTA
PRIMER_LEFT_3=1063,22
PRIMER_RIGHT_3=1232,21
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.978
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=1.2582
PRIMER_LEFT_4_PENALTY=0.236697
PRIMER_RIGHT_4_PENALTY=1.021538
PRIMER_LEFT_4_SEQUENCE=ACCGGAATAATTGTCGAGTTTG
PRIMER_RIGHT_4_SEQUENCE=CGGGGACACAATTTCAGAGTA
PRIMER_LEFT_4=980,22
PRIMER_RIGHT_4=1232,21
PRIMER_LEFT_4_TM=60.237
PRIMER_RIGHT_4_TM=59.978
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_03344 Ps_contig_mira-and-tgicl-ass_franssen_29630
SEQUENCE=CCTTAAAAATATCTCACCACTATTATATGGTATGATCCACAATTCTNTGAAATAAAATAAATTAAACATCAAGAATAAAATTGGATTTTGCACAACAAACTAGGGAATAAAATAATTCACATAAAACCATAGCAAAACAAATTAATATATTACAATGTGATGCAAAACATGAAGAACATGTTCACAAATTCTTCCCTTGGAAACTACTTCAAATTTTCATGCATTATGCACCAAAACATCTATCCTAATGGAACTCTCCATTTTTTTCAATGGTGGTGGCGGACACATTCGGCGGGGATGACGGGAAACCTAGCGGTATCGGCGCAATAATCGTAAACCATATGATTAGCCCTAACCCAAATAAGTTGATGACTTTGATGCAAAGTGAGTTCAGACAACATAGGTTCATCCCACCAAAATTTCTTATCCTCACTACTACTACATTTCTTAGCATTATCAATGGATGTAACAGGTGTTGGACATTCACAAGCATTGATTTCGAAATCTTTATAAGTAGCAACAAAAGGTGCATGAGACCAATCTGTTTTCACACGACCACCTTGTGTGGCCCAATCATCAGCATTCCATATTGAACTATAAACACCCATTGCTTGGTCTTTGGGGAAAGGGATTCCTTTGTGTTCCAAATTTGTGTGTACTCTAATTGGTGTCTCATCCACTAGAAACACTACTTGACGTTGGTTCCAAAAGATTGAGTAGGTGTGAAAATCCTTAGATGGATCAAACCATAGGTTAAGTCTTTGTTCTCTATTACCAACACCATTCACATACACATTTGTTTGAACTGAGTAAGGTTCACCAGTTGTGTTGCCCAAAAACTCAAAATCAAATTCATTATGAGTTGGTCCTTCTGATGACATATAGAAAGCTGTAACAGTTCCAGCAGAGTCACCTTCCACAAGCTTAAGCTGAACAGTCACTTTTCCAAACATATATTTGCTCTTAGATCCAAATCCAGCACCGGAATAATTGTCGAGTTTGAGTTTGAGTAAATCTCCTTCATGAACGAAATGGTCCATAGCCCAACCAGGTTGAAAAAGCTCCTCAAATTTGGAAGAAGCAACTAATGCAACAAACAAAAAACTCAGAGAAAGTCCTAAGAGACAAGACATTTTTGTCATGTTGAAAAACACAGACAAAAAAATCGATGAAGAAGAAGAAAGAGAGAGTGAGTGAGTGAAGTGAAGCTTCTACTCTGAAATTGTGTCCCCGACGTA
TARGET=1188,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0024
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.893511
PRIMER_LEFT_SEQUENCE=CCTCAAATTTGGAAGAAGCAAC
PRIMER_RIGHT_SEQUENCE=CGGGGACACAATTTCAGAGTAG
PRIMER_LEFT=1063,22
PRIMER_RIGHT=1232,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.894
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=1.1302
PRIMER_LEFT_1_PENALTY=0.236697
PRIMER_RIGHT_1_PENALTY=0.893511
PRIMER_LEFT_1_SEQUENCE=ACCGGAATAATTGTCGAGTTTG
PRIMER_RIGHT_1_SEQUENCE=CGGGGACACAATTTCAGAGTAG
PRIMER_LEFT_1=980,22
PRIMER_RIGHT_1=1232,22
PRIMER_LEFT_1_TM=60.237
PRIMER_RIGHT_1_TM=60.894
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=1.1302
PRIMER_LEFT_2_PENALTY=0.236697
PRIMER_RIGHT_2_PENALTY=0.893511
PRIMER_LEFT_2_SEQUENCE=CACCGGAATAATTGTCGAGTTT
PRIMER_RIGHT_2_SEQUENCE=CGGGGACACAATTTCAGAGTAG
PRIMER_LEFT_2=979,22
PRIMER_RIGHT_2=1232,22
PRIMER_LEFT_2_TM=60.237
PRIMER_RIGHT_2_TM=60.894
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=1.1305
PRIMER_LEFT_3_PENALTY=0.108929
PRIMER_RIGHT_3_PENALTY=1.021538
PRIMER_LEFT_3_SEQUENCE=CCTCAAATTTGGAAGAAGCAAC
PRIMER_RIGHT_3_SEQUENCE=CGGGGACACAATTTCAGAGTA
PRIMER_LEFT_3=1063,22
PRIMER_RIGHT_3=1232,21
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.978
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=1.2582
PRIMER_LEFT_4_PENALTY=0.236697
PRIMER_RIGHT_4_PENALTY=1.021538
PRIMER_LEFT_4_SEQUENCE=ACCGGAATAATTGTCGAGTTTG
PRIMER_RIGHT_4_SEQUENCE=CGGGGACACAATTTCAGAGTA
PRIMER_LEFT_4=980,22
PRIMER_RIGHT_4=1232,21
PRIMER_LEFT_4_TM=60.237
PRIMER_RIGHT_4_TM=59.978
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_03345 Ps_contig_mira-and-tgicl-ass_franssen_29636
SEQUENCE=TTCGRGGATTCTTTCTTTATTTTTTATTTATTTATTTTCCTCATAAGAGGAGCCTCTGGATCCATCTTCTAGAATCGAAGACCCTTGAGATTTCGCCACCATGAGTCTCTTCGGTCTTGGAAGCAGAAACCAAAAAACATTTCGTCCAAAAAAGAGTGCTCCCACTGGAAGTAAGGGTGCTCAACTTCAAAAACACATTGATGCTACATTGGGTAGTGGGAACCTGAGGGAAGCTGTTAAACTCCCTCCTGGTGAAGATATCAATGAGTGGCTAGCTGTAAACACTGTGGACTTCTTTAATCAAGTGAATATCATGTTTGGTACGTTGACTGAATTCTGCACGCCGAGTAACTGTCCTACAATGACTGCAGGGCCAAAGTACGAGTATCGATGGGCTGACGGCGTTACTATCAAAAAACCAATAGAGGTATCTGCTCCAAAGTATGTCGAGTATTTGATGGATTGGATTGAATCTCAACTAGATGATGAAACAATATTCCCTCAAAGATTAGGGGCTCCTTTTCCACCCAATTTCAGAGATGTTGTCAAGACAATATTTAAGCGATTATTCCGAGTATATGCTCATATTTATCACTCACATTTTCAGAAGATTGTAAGTTTGAAGGAAGAAGCTCACTTGAATACTTGCTTCAAGCATTTTGTTTTATTTACTTGGGAGTTCCGGTTGATCGAAAAAGCGGAGCTAGCACCTCTTGAGGACCTTGTTGATTCTATTATACAATGATAGTGTTTTTTATTTGCCTTCTCTTTCCTTCATTTTGGAGAGGTTAGACAATGGAAGGTTGGGTATATTTTTCTTTTTGTGGGCTTATTATCATGCCCTTGTAATATAACTTGCTAAATGGAAAATCTTTAGCCACTTTTTTTGTATTTGTGTCTGCATTGCTGTTATGGTTGCTGAATATTCTTGTGCATCAAGCTAAACCTACTTGTGCAGCGAGCCGAATAGTCGATGCATTGACCAGTTGTATTTTCTATCAATTATTTTAAGGTGGCATTATAAATTGTGATTTTCTGGG
TARGET=23,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03346 Ps_contig_mira-and-tgicl-ass_franssen_29648
SEQUENCE=AAAAGGTTGTAAAAATTTATTTCACACTTGTAGTATAACTTACAATCAAGGATCTAATAATTTAATATAAGCTTCTAATTACTTATGAGAAGATAATGTCATTGAAAGGTGGATAACATTTTTAGGTCTAGAACAACATATATTTTTTATAAAAAAACAACCGATTATGAAAACAACGCATCCAAAAGAACACCCACATAGATAACAGATGAATAAACCATGGAAACAAGAATCTGAAATAGATTCTAGAAATCATCAGGATCTTCTTCACCACCCTCGCCAGATTCAGCACCAACTTCTTCATAATCCTTTTCCAGAGCAGCAAGATCCTCACGAGCTTCAGAAAATTCACCTTCTTCCATACCCTCGCCCACATACCAGTGAACAAAAGCACGTTTGGCATACATGAGATCAAACTTTTGATCTATGCGACCAAACACCTCAGCTACACTGGTGGAGTTTGAAATCATGCAAACAGCTCTCTGTACTTTAGCAAGGTCGCCTCCAGGAACAACAGTGGGTGGCTGGTAGTTAATACCACACTTGAAACCAGTAGGGCACCAATCAACAAACTGAATGGTCCTCTTTGTTTTGATGGTTCCAACAGCGGCGTTCACGTCCTTGGGAACAACGTCACCACGGTACATCAGACAACATGCCATGTACTTTCCATGGCGAGGATCACACTTGGCCATCATGGAGGATGGCTCGAAAGCACTGTTGGTAATTTCAGCAACTGAAAGCTGCTCGTGATATGCCTTCTCAGCTGAGATAACGGGAGCATATGAAGAAAGCATGAAATGGATTCTAGGATAAGGGACCAAGTTGGTCTGGAATTCAGTAACATCAACATTGAGGGCACCATCAAACCTCAGAGAAGCAGTCAAGGATGAAATCACCTGGGAGACTAGGCGGTTGAGGTTGGTGTAGGTAGGACGCTCAATATCAAGAGATCGCTTGCAAATATCATAGATAGCTTCATTGTCCAAAAGAACAGCAACATCGGTGTGCTCCAACAGGGAGTGAGTGGAGAGGACACTGTTGTATGGCTCAACAACAGATGTGGAAACCTGAGGTGAGGGATAGACAGTGAACCCTAGCTTCGACTTCTTTCCATAATCAACAGAAAGACGCTCCAAGAGAAGAGAACCAAGACCAGAACCAGTTCCTCCACCAACAGCATTGAACACCAAAAACCCTTGGAGACCAGTGCAGTTGTCAGCAAGCTTTCTGATCCGGTCCAAACACAGATCAACAATCTCTTTCCCAATGGTATAATGACCACGGGCAAAGTTGTTGGCAGCATCTTCTTTACCGCTAATGAGCTGCTCTGGGTGGAAAAGCTGGCGATATGTCCCAGTCCTCACCTCATCAATAACAGTGGGCTCCAGATCTACAAAAACAGCTCGGGGTACATGCTTTCCAGCACCTGTCTCGCTGAAGAAAGTGTTGAAAGCATCATCACCTCCACCAACAGTCTTGTCACTTGGCATTTGGCCATCGGGACCAATGCCGTGCTCGAGACAGTAAAGCTCCCAGCATGCATTTCCGACCTGAATACCGGCCTGACCAATGTGAACGGAGATGCACTCTCTCATTTTCGGAGACGAAAAAACGAAAGAAATGGAAAGAGAAAACTGAATGGATAGAAGATATACGAGAGAGAAGATATACGAGAGAGAGAAAAAGGGAAGACGTTTATGAAGACGCCTTCTTCTAGATCTGGATCTGATAATCNAGCCGTACTCTGCG
TARGET=1675,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1036
PRIMER_LEFT_PENALTY=0.086698
PRIMER_RIGHT_PENALTY=0.016950
PRIMER_LEFT_SEQUENCE=GAGACAGTAAAGCTCCCAGCAT
PRIMER_RIGHT_SEQUENCE=TCCAGATCTAGAAGAAGGCGTC
PRIMER_LEFT=1520,22
PRIMER_RIGHT=1726,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=59.983
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.1851
PRIMER_LEFT_1_PENALTY=0.168114
PRIMER_RIGHT_1_PENALTY=0.016950
PRIMER_LEFT_1_SEQUENCE=TCTCGCTGAAGAAAGTGTTGAA
PRIMER_RIGHT_1_SEQUENCE=TCCAGATCTAGAAGAAGGCGTC
PRIMER_LEFT_1=1432,22
PRIMER_RIGHT_1=1726,22
PRIMER_LEFT_1_TM=60.168
PRIMER_RIGHT_1_TM=59.983
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=0.2322
PRIMER_LEFT_2_PENALTY=0.215254
PRIMER_RIGHT_2_PENALTY=0.016950
PRIMER_LEFT_2_SEQUENCE=CAACAGTCTTGTCACTTGGCAT
PRIMER_RIGHT_2_SEQUENCE=TCCAGATCTAGAAGAAGGCGTC
PRIMER_LEFT_2=1471,22
PRIMER_RIGHT_2=1726,22
PRIMER_LEFT_2_TM=60.215
PRIMER_RIGHT_2_TM=59.983
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.2927
PRIMER_LEFT_3_PENALTY=0.275745
PRIMER_RIGHT_3_PENALTY=0.016950
PRIMER_LEFT_3_SEQUENCE=ACGGAGATGCACTCTCTCATTT
PRIMER_RIGHT_3_SEQUENCE=TCCAGATCTAGAAGAAGGCGTC
PRIMER_LEFT_3=1578,22
PRIMER_RIGHT_3=1726,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.983
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.2976
PRIMER_LEFT_4_PENALTY=0.086698
PRIMER_RIGHT_4_PENALTY=0.210886
PRIMER_LEFT_4_SEQUENCE=GAGACAGTAAAGCTCCCAGCAT
PRIMER_RIGHT_4_SEQUENCE=AGGCGTCTTCATAAACGTCTTC
PRIMER_LEFT_4=1520,22
PRIMER_RIGHT_4=1711,22
PRIMER_LEFT_4_TM=59.913
PRIMER_RIGHT_4_TM=59.789
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_03347 Ps_contig_mira-and-tgicl-ass_franssen_29653
SEQUENCE=CAACTCTTCTAATTTTTCTTTTCATATTATTAATGAATGCTAAATTTGTAAAGAAAATCACAATTTGGTTTTCCGATACAAAATTAACTATATAATGTATTTCTTCATTTCACGTATGAAGTAATAAAACAACATATACTTTTGAAGAAGAGAGTATCTTACTTTCATATATAATTTTCACAAATACACAAGAAACATTAAAGTTCTCCAACATCCGACTGATCATCTCCAGAAAACAAAGGCAAGCAAGAAAACAACAGTCAATAGCATTCCTGCATATTTGATCCATTGATCAACTCGGTTTCGTCTCTCAATTAATCTCAGAACTCTGTTTGATATCCCTACTGTATTCAGGACGTCCAATGCCTTTCGGTGTGCGCTCTTCAAGCGTTCCCTTTGGCCGTGTATTGAAGAAAGGATCGCTTCCCCGAGTGCATTAGCATTTTCAAGCTCCCTGGCAGAGCTGCGAACTGAGTGCATTGCTTGCGTGTCATCGTCATAAATTCTCAAAACGTGAGCAGAATCCCCATTCATTCGTCCCAGCAGCTCTGCTCTCTCTTTGGCTTCTCTAGACCGTTTCTGACTCCTTGAGTTATATTTATCCAAACTTTCTTTCAGTGATTCAGCCTCTTCAGCTATTTGTTCAACCTTTCTTTTCCAGAGGTCACGTTGAGGTTTGGCGGCGACGGAACGCCAAAGCCGGTCCATCTCGACGCAGAGGAATTGAATCTGGTTAATATCCTTCTTTAAGGTAAATGAAAGCTCCAAATCAACGCCGGAAATGGCAGCGGAGGTAGAGGTGGAGTGCTCGAGACGCTCAAGCCGTTCGATGCCGTCGCGGCACCTGAGCAATAGCTTCTTTGCGCTTTGGGNACTTCCGGAGNCTTCCTCCGCCTCCTCCTTNCGTCCCTTCCCTTCCCTTACCAAGTTCGGGTAAACCGGACGGGTTGTAGTTTGGG
TARGET=907,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.6861
PRIMER_LEFT_PENALTY=0.048131
PRIMER_RIGHT_PENALTY=3.638001
PRIMER_LEFT_SEQUENCE=GCACCTGAGCAATAGCTTCTTT
PRIMER_RIGHT_SEQUENCE=GTCCGGTTTACCCGAACTTG
PRIMER_LEFT=840,22
PRIMER_RIGHT=943,20
PRIMER_LEFT_TM=60.048
PRIMER_RIGHT_TM=61.638
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=4.3828
PRIMER_LEFT_1_PENALTY=0.744773
PRIMER_RIGHT_1_PENALTY=3.638001
PRIMER_LEFT_1_SEQUENCE=AACCTTTCTTTTCCAGAGGTCA
PRIMER_RIGHT_1_SEQUENCE=GTCCGGTTTACCCGAACTTG
PRIMER_LEFT_1=645,22
PRIMER_RIGHT_1=943,20
PRIMER_LEFT_1_TM=59.255
PRIMER_RIGHT_1_TM=61.638
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=299
PRIMER_PAIR_PENALTY_2=4.3828
PRIMER_LEFT_2_PENALTY=0.744773
PRIMER_RIGHT_2_PENALTY=3.638001
PRIMER_LEFT_2_SEQUENCE=CAACCTTTCTTTTCCAGAGGTC
PRIMER_RIGHT_2_SEQUENCE=GTCCGGTTTACCCGAACTTG
PRIMER_LEFT_2=644,22
PRIMER_RIGHT_2=943,20
PRIMER_LEFT_2_TM=59.255
PRIMER_RIGHT_2_TM=61.638
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=4.4608
PRIMER_LEFT_3_PENALTY=0.822824
PRIMER_RIGHT_3_PENALTY=3.638001
PRIMER_LEFT_3_SEQUENCE=CACCTGAGCAATAGCTTCTTTG
PRIMER_RIGHT_3_SEQUENCE=GTCCGGTTTACCCGAACTTG
PRIMER_LEFT_3=841,22
PRIMER_RIGHT_3=943,20
PRIMER_LEFT_3_TM=59.177
PRIMER_RIGHT_3_TM=61.638
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=4.6669
PRIMER_LEFT_4_PENALTY=1.028939
PRIMER_RIGHT_4_PENALTY=3.638001
PRIMER_LEFT_4_SEQUENCE=AACCTTTCTTTTCCAGAGGTCAC
PRIMER_RIGHT_4_SEQUENCE=GTCCGGTTTACCCGAACTTG
PRIMER_LEFT_4=645,23
PRIMER_RIGHT_4=943,20
PRIMER_LEFT_4_TM=60.029
PRIMER_RIGHT_4_TM=61.638
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_03348 Ps_contig_mira-and-tgicl-ass_franssen_29657
SEQUENCE=ATGCAAAGATAAAAGTAACRTCCAGCATTATTTCACACATCCATCAACAGAAATGTGTTCATATATCCACAAGCCCAATATTTTCCTCTCTCACCTACTTAATTTATTAAAACATGTTTTTTTCTTCAAGTTGTGGATGTCTCTACAATTAGTCAAAGTTTCACATTTAGTAATATGAAACTTGCATAACAAGGAATGAAGTAGATTCATTAACTCAAACAAGCAACAACAACAACATTAAAGACTAATAATAACAATAATGAAAAAGACAGTAATTGTTTCATGTCTCAGCTGTGTCTGAGCTTATTACAGTCTCTTCTAGTGATGATGTTGGTAATTTGGTCCCTGAGCTCATTTTGTGCATATCTTGGCTTGAACAAATGAAGATCCTTTTCACCATATTGCAAAATTCCGGCCATGGATCGTCGCCAACAAGCATCAAATCCCCTTCATCATCAGTGAAGACGATTTCCCACTTATTCCTAGGTTGGAGCTGTCCCTTTATGTCAAACAGCTTCTCCAATTCATCGATAAGTTGATCATATCCATCCAATGCAGTCAAGTACACCGCCCGACCCACTGCAACACCCTGCATTTGAACCTTAGTGCAACTTCTGCTATTGATTTGCTTGCTTAGTGTTTCGTTCGGAGATTGTTCTTGTTTCCTTTCTTTTGAAGTCTTTGAGATATCAAACTTATTTTGGTCCGCATCAGTTCTTGACAAGGGAGTGACAACGCATCTCTCGGACGTGACTCCCGATGCATTCGGAGAATTTGTGCAAGGATCATTAAGATAACTCCCGAACACTCTACATCCTCTAGCTGTTTCTAATTTGTTCTCCTTGTCAACTTGGTCAATCAGATTATCATTTTTGTTCAATATTTCTGAGTGAGTTTTCAAAATAGGCCAAGCTGAGACGGTGACACTCTTGCTAGTGTCGGTCGTGTCATGACACAAATGAGAAGGACCACTAGAGTGAGGAGAAGATAGCCAACTTCCGTCTGACCCCC
TARGET=224,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03349 Ps_contig_mira-and-tgicl-ass_franssen_29658
SEQUENCE=GGCCAAATTTCCATCTTTCTCCTTTNGGTNNACACACACCTCATCAATGTTGAAAGTTGAAAGTTGAAACCAAACACCTCTTCATTAAAATTGCCCAATTCAATTTCATGTTATCTCTCCGCTTATAATTCAATATCTATTTCACTTCATAAATTCACACCAAAGAGATTAATCATGGAAGGAATAGAACACAGAACAGTTGAAGTGAATGGAATCAAGATGCATATCGCAGAAAAAGGAAAAGAAGGGCCGGTTGTTTTGTTCCTTCACGGCTTCCCTGAACTCTGGTACTCATGGCGCCACCAGATTGCGGCTCTCGGTTCCCTCGGTTACCGCGCGGTGGCTCCGGATCTCCGTGGCTATGGAGATACCGAAGCTCCCGCTTCGGTAAGTAGCTATACGATTTTCCATTTGGTTGGTGATGTTATTGCTCTGATCGATTTACTCGGTGCTGATCAAGTCTTCCTTGTGGGTCATGATTGGGGTGCTATTATTGGTTGGTATGTTTGTTTGTTTCGCCCTGAGAGAATCAAAGCTTATGTGAGTCTCAGTGTTCCTCATTTACCGAGAAACCCTAAAATCAAACCTGTTGATGG
TARGET=504,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1463
PRIMER_LEFT_PENALTY=0.132857
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=TTGGTTGGTGATGTTATTGCTC
PRIMER_RIGHT_SEQUENCE=TAAGCTTTGATTCTCTCAGGGC
PRIMER_LEFT=411,22
PRIMER_RIGHT=538,22
PRIMER_LEFT_TM=59.867
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=0.4930
PRIMER_LEFT_1_PENALTY=0.479611
PRIMER_RIGHT_1_PENALTY=0.013401
PRIMER_LEFT_1_SEQUENCE=GATCGATTTACTCGGTGCTGAT
PRIMER_RIGHT_1_SEQUENCE=TAAGCTTTGATTCTCTCAGGGC
PRIMER_LEFT_1=434,22
PRIMER_RIGHT_1=538,22
PRIMER_LEFT_1_TM=60.480
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=0.5146
PRIMER_LEFT_2_PENALTY=0.501224
PRIMER_RIGHT_2_PENALTY=0.013401
PRIMER_LEFT_2_SEQUENCE=TGGTTGGTGATGTTATTGCTCT
PRIMER_RIGHT_2_SEQUENCE=TAAGCTTTGATTCTCTCAGGGC
PRIMER_LEFT_2=412,22
PRIMER_RIGHT_2=538,22
PRIMER_LEFT_2_TM=59.499
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=127
PRIMER_PAIR_PENALTY_3=0.5237
PRIMER_LEFT_3_PENALTY=0.020747
PRIMER_RIGHT_3_PENALTY=0.502921
PRIMER_LEFT_3_SEQUENCE=GATCAAGTCTTCCTTGTGGGTC
PRIMER_RIGHT_3_SEQUENCE=TAGGGTTTCTCGGTAAATGAGG
PRIMER_LEFT_3=453,22
PRIMER_RIGHT_3=576,22
PRIMER_LEFT_3_TM=59.979
PRIMER_RIGHT_3_TM=59.497
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=0.5673
PRIMER_LEFT_4_PENALTY=0.064372
PRIMER_RIGHT_4_PENALTY=0.502921
PRIMER_LEFT_4_SEQUENCE=ATGATTGGGGTGCTATTATTGG
PRIMER_RIGHT_4_SEQUENCE=TAGGGTTTCTCGGTAAATGAGG
PRIMER_LEFT_4=475,22
PRIMER_RIGHT_4=576,22
PRIMER_LEFT_4_TM=59.936
PRIMER_RIGHT_4_TM=59.497
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=102
=
PRIMER_SEQUENCE_ID=SSR_03350 Ps_contig_mira-and-tgicl-ass_franssen_29661
SEQUENCE=AATTAATCATCTTAGTTTTAACTTAAACTTGGTTGTAACTGTATACACACTGTAATAGATATTGTTTAGATACTGCAATATTTCAACACCCAAATATGGTGTCTCTTTGATATTTTGATCACAATCTGATCAAATTCTGAAAACACATGTCGTTTTTTATTTATTGGTTTTGTATGTTCCCAACACATTACTACTTTACAACAAAATTATACAACTCTAATTCAAGGCTATGCCCATTTTCAACCTCCAATACAGTGTCATATTATATTGATCTTTTAGGATGAACTCACATTGTTTTTATAAGAATTAACCATAGCACCGAATTGGGTCATTGCATCCTTTAACAGCAGATTGCTTGAAACCAAATCGACCGCCTTTCTCAAGATACTGTTGATGGTACTCCTCGGCTCTGTAGAACTTCTTAGCAGGAAGGATCTCAGTAACAATCTTTCTGCCCAATTGCTTCTCTTGTTCTTCCAATGATTCTCTAGCCGCTTTCTCTTGTTCAGGAGTGTAGTAGTATATTCCAGATCTGTACTGTGTTCCGACATCATTCCCCTGACGATTTGGCGTAGTAGGATCATGTTTAGTCCAGAACGTATCGAGCAGATTTTCAAAGGCACATTGTTTGGGATCATATTGAACCCTTACAACCTCTGAGTGGTTTGTGGTCCCTGCACACACATCTTCATACGCCGGATTATGCACAAGCCCCTGGGTGTAACCAACCTCTGTCTTACTCACTCCCGGCACCCTCTGGAAGACCAATTCAACACCCCAAAAGCAGCCAGCACCAAATTGAGCAAACTGCAGGCCTGGTGCTGGTATATCTTCATCTGGTCCCTGAGGAATGGCTGGATCCAGATTCTCTGATGACCTTCCACTGCCAAATCCAAGTTTGTTCAGAAGGTTCATGGAGGGCTTGGTCACAGTAGAATAGGGTCGGGTCTGTGAAGAGAAGCAAGAACGCTTGACAGAAAATCTAGAAAGTGAGGGTAGGAACTTGGTTTTGGCTGGAGTAGAGAAGGAAGAAGAAACAAACACTTGCAGGGAATTAGACGCAGTGCTATAGGTGCTGCTGGTTGCTGCTCCACAAATTCTCATCTTCAACCCCGAGAGAGAGAGAGAGCNYYAGTGGTTGTTAGNGTGAGAGAAGAAGAGNNGAGATTCTCACGGAAAC
TARGET=1115,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03351 Ps_contig_mira-and-tgicl-ass_franssen_29668
SEQUENCE=ATGGGGGAANAAAACCCACACTTGTCCTTACGTGCCTCTTCGGGCAGCCCGTAATTCGATTTTTCAGTTTTATGAATGCTGCCCTAACTGCCCGARRCGNGGGCTGAGAAATTACGAGCAGAACCTGTGTCTCCTTCTCTCTTTCTCTCACTGGCCTCTGATATTTCTATATTTCGAAGCTCAGTGTTTCATCGATCAAGATCCAACGGTCCATAATGTACGAGCGTTCTTCTCCTATTTTTCAGAGGTTTTAGTGCTTCAAACCCCAGATCTTGCTTATCAGTCTCTAGATCTTCGTTCCTGGATAAAAATTTTCTGGTTTGAGAAAAGACTTGTTTGTTTGTCACACGAAATGGAGACTGAATACAGTTCGATGATGGTCACGCGCATGGTCACGCGCGGCGCGTTAACGTACGCAGCGGAAGTGCTTGTGACTTTGTTAGCTTCCGCAGTCGTGAAAGTCACGCTTTTGGCTCCGGAACTCTATGAGCTAATGCTAACCAACATGCCTACAACAAAAGGAGAGAGTAAAACCCCAAGTCAAGGCAAGCAACATGACGCTGAAGGAGAAGATGGCAATGACGATGAAGATGAAGAAGACGACGACGGAAATGGTGCATTTGGAGAAGGAGAAGATGAATTGTCTTCTGAAGATGGTGGTGGATATGGCAACAATTCAAACAATAAGAGCAACTCAAAGAAGGCACCTGAAGGCGGTGCTGGTGGGGCAGACGAAAATGGAGAAGAGGAAGATGATGAAGATGGCGATGACCCAGATGAAGACGATGACGAGGACGAGGACGATGATGATGAGGGACGGAGGGTGGAGAAGAGGACGAAGGAGGAAGGGTTGATGAAGAAGAGAATGAAGAGGAGTGGAGGGAAGGAAGGAAAGACGAGGAGCTTGCACGACCAACCGAAAAGAAA
TARGET=333,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03352 Ps_contig_mira-and-tgicl-ass_franssen_29668
SEQUENCE=ATGGGGGAANAAAACCCACACTTGTCCTTACGTGCCTCTTCGGGCAGCCCGTAATTCGATTTTTCAGTTTTATGAATGCTGCCCTAACTGCCCGARRCGNGGGCTGAGAAATTACGAGCAGAACCTGTGTCTCCTTCTCTCTTTCTCTCACTGGCCTCTGATATTTCTATATTTCGAAGCTCAGTGTTTCATCGATCAAGATCCAACGGTCCATAATGTACGAGCGTTCTTCTCCTATTTTTCAGAGGTTTTAGTGCTTCAAACCCCAGATCTTGCTTATCAGTCTCTAGATCTTCGTTCCTGGATAAAAATTTTCTGGTTTGAGAAAAGACTTGTTTGTTTGTCACACGAAATGGAGACTGAATACAGTTCGATGATGGTCACGCGCATGGTCACGCGCGGCGCGTTAACGTACGCAGCGGAAGTGCTTGTGACTTTGTTAGCTTCCGCAGTCGTGAAAGTCACGCTTTTGGCTCCGGAACTCTATGAGCTAATGCTAACCAACATGCCTACAACAAAAGGAGAGAGTAAAACCCCAAGTCAAGGCAAGCAACATGACGCTGAAGGAGAAGATGGCAATGACGATGAAGATGAAGAAGACGACGACGGAAATGGTGCATTTGGAGAAGGAGAAGATGAATTGTCTTCTGAAGATGGTGGTGGATATGGCAACAATTCAAACAATAAGAGCAACTCAAAGAAGGCACCTGAAGGCGGTGCTGGTGGGGCAGACGAAAATGGAGAAGAGGAAGATGATGAAGATGGCGATGACCCAGATGAAGACGATGACGAGGACGAGGACGATGATGATGAGGGACGGAGGGTGGAGAAGAGGACGAAGGAGGAAGGGTTGATGAAGAAGAGAATGAAGAGGAGTGGAGGGAAGGAAGGAAAGACGAGGAGCTTGCACGACCAACCGAAAAGAAA
TARGET=882,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03353 Ps_contig_mira-and-tgicl-ass_franssen_29669
SEQUENCE=GTAGCCAGTGAAGTRGGRAAAGTCANNATAAAACAAGCATCATGTATTTTTCTATCGTTAATTTAGGACTGTCTGCTCAATATAATGTAGCATTCTAATTACGCAATGGTAAACACAGATATCAATTAAAACATGTCCTACTACATAGGCCTCCCTTATAATGTAAATAACAACATAGAAGTTTAGAACCGAATAAAACAACATGGAAGTTAGAAGCCAAATCCAAACACAACAATACAAGAAGGAGGCGTCACTAAAACCTAAATTCACTTCTTCCTTTTCTTTGGTGGCTGCAAAGCTTCCTCGTCTTCCTCCTCCTCATCCTCTTCATTCTCTTCTTCATCAACCCCTTCCTCTTCGTCCTCTTCTCCACCCTCGTCCTCATCATCATCGTCCTCGTCCTCGTCATCGTCTTCATCTGGGTCATCGCCATCTTCATCATCTTCCTCTTCTCCATTTTCGTCTGCCCCACCAGCACCGCCTTCAGGTGCCTTCTTTGAGTTGCTCTTATTGTTTGAATTGTTGCCATATCCACCACCATCTTCAGAAGACAATTCATCTTCTCCTTCTCCAAATGCACCATTTCCGTCGTCGTCTTCTTCATCTTCATCGTCATTGCCATCTTCTCCTTCGGCGTCATGTTGCTTGCCTTGACTTGGGGTTTTACTCTCTCCTTTTGTTGTAGGCATGTTGGTTAGCATTAGCTCATAGAGTTCCGGAGCCAAAAGCGTGACTTTCACGACTGCGGAAGCTAACAAAGTCACAAGCACTTCCGCTGCGCAGGTTAACGCGCCGCGCGTGACCATCATCGAACTGTGTTCAGTCTCCATTTCGTGTGACAAACAAACAAGTCTTTTCTCAAACCAGAAAATTTTTATCCAGGAACGAAGATCTAGAGACTGATAAGCAAGATCTGGGGTTTGAAGCACTAAAACCTCTGAAAAATAGGAGAAGAACGCTCGTACATTATGGACCGTTGGATCTTGATCGATGAAACACTGAGCTTCGAAATATAGAAATATCAGAGGCCAGT
TARGET=839,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03354 Ps_contig_mira-and-tgicl-ass_franssen_29678
SEQUENCE=AAAGTTTTAGANGTTGACCAGCAAAAGANTNTAAATATGAACAAACACTCAATTTCTTGCCACTTCTACAAACAAGACCGTTTAGGATCATGTCTGGTAAGTCAGTGGTAAGGTATTTAACAGTAGTATACAACATAGCTACTTTCAGTACTAAGAATCATTTATCCATATCTCTTCCCTGAACAATTGAATGGCAAAATATAAATTGAAAGAGACAACTGTTTAAGGCTATTCACTAACACTGCAGGGCAACATTTCAAATGAACCTTCCAGGTAAGGCAACTTCAGTTCAAAACCACATATTAAGGAGCTCCGGAAGAACCAATATGTGGGTCTCCTTAAACCTTTAAAACTGCTTTATGGACCATGGTTTCCTTGTGTATTTGATGACAGCTCTTCTCAACAAGATCAAGCAGTTTCTCTAAATTTGCTGCCCATGAATTAAGAATATCATTGCTATCCTTTGCTGTTTGGAAACAAACTATTCCCATTGGTCTATCAATCTTTGCCACCAGAGCCTTCGACACCACCATTTCAGAGAGATGCTTCTCAGCTTCCTCTAGGCTGAGACACAAAAGCTGTGCAAGTCTCTTCAAAGTAATCCTTGCATAGTATTTTGATACAACGAGAATATTATGTTCAATAATTCTTTGTTTCAGATCTTCTGCTGCTTTTTCACTCAAAAAATTTCCAGAGACCATCTCACTGTCAAACTCGTTCTTATATGTATCCCAAAGAGCTGTCCATTGGATGACTTCCATGGTTACCACATTCTTCAAAAGTAGCTTGAAGTTTGGAATCTCAGAAAGATTCCTATCATCCAATGTGGAATTGAGAAGACTCGACTGCATTGGTTCATGTGGTGCAAGAACCAAGAACCAACATATTTTCCTCAGGATAGGAATAAACTCAGCAGGGTTTTCTTTGACAGATGGAATCTCATATATTGCCTTGTAGCAACGGCAAATTTCAAGATATTCATTGTTGTGGGAATAGTACCTAATCATTAGTTCATAATAAATCCGCTTCAACTCCAGTAGAGATGGAATGTCTGCAGGAGCCTCTTCAACCATATTATCGCCTTCTTTAGACTTTTTCTTTTCCTTAGACGCATCTATATCGAAAACTCTTGGACTAATCTTTCTTGAGAGTATTTGTGCACGAACATAATCTTGACGATCTAGACACAATCGAACTTGTTCAAGAATGAAGGCAATTTTCTCAGTTTTTGCCATGGCACCAAAAGTTTCCACCGCAATTTCTTGCATCAAATCAGCAGCCTCGGCTATAAGCCCTTGTTCTTCCTTAATCTTAGCAAGTTTCTTGACCAACCGAGCTCTCTCAATCTCAACATAGATCTTCCCTGCAGATACACTGTTCAATGTTTTGATAAGTTCTATACGAGTTTCAATATCAGGTGTCTCATCAATATATTGCATAGCCTGCTGGACCATAGCTGTTACAGCCTGCTTAAGCTGACCGCGTCGTTTAGACAAAACGACAATCTGATCATTTAGGGTTTTCCATGCTTTCGCTTCGAAGCAGAGTTGCAAAATGTCGATTGCGGCTTTTCTGGTGCCGACAATTTCACCGGCGAGCCGCATCTGCTTCTCCACGTTCAGCAATTGCTCTATCTTCGCATCTAAATTCTCCGTATTCGCCATGGTTGAGTGAGGTTTGGGCGAGAGAAAGAGAGAGAGATCTATGAAGGAATCGGCGATATTGGAAATTAGAAAAAGGAAGAAAATGTGAGAGAGTCCCCGCGTACTCTGCGTTG
TARGET=1691,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3938
PRIMER_LEFT_PENALTY=0.315086
PRIMER_RIGHT_PENALTY=0.078668
PRIMER_LEFT_SEQUENCE=GATCATTTAGGGTTTTCCATGC
PRIMER_RIGHT_SEQUENCE=ATATCGCCGATTCCTTCATAGA
PRIMER_LEFT=1507,22
PRIMER_RIGHT=1722,22
PRIMER_LEFT_TM=59.685
PRIMER_RIGHT_TM=59.921
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=0.7472
PRIMER_LEFT_1_PENALTY=0.668546
PRIMER_RIGHT_1_PENALTY=0.078668
PRIMER_LEFT_1_SEQUENCE=CAGAGTTGCAAAATGTCGATTG
PRIMER_RIGHT_1_SEQUENCE=ATATCGCCGATTCCTTCATAGA
PRIMER_LEFT_1=1542,22
PRIMER_RIGHT_1=1722,22
PRIMER_LEFT_1_TM=60.669
PRIMER_RIGHT_1_TM=59.921
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=0.8179
PRIMER_LEFT_2_PENALTY=0.739274
PRIMER_RIGHT_2_PENALTY=0.078668
PRIMER_LEFT_2_SEQUENCE=TATATTGCATAGCCTGCTGGAC
PRIMER_RIGHT_2_SEQUENCE=ATATCGCCGATTCCTTCATAGA
PRIMER_LEFT_2=1429,22
PRIMER_RIGHT_2=1722,22
PRIMER_LEFT_2_TM=59.261
PRIMER_RIGHT_2_TM=59.921
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=294
PRIMER_PAIR_PENALTY_3=0.8487
PRIMER_LEFT_3_PENALTY=0.315086
PRIMER_RIGHT_3_PENALTY=0.533658
PRIMER_LEFT_3_SEQUENCE=GATCATTTAGGGTTTTCCATGC
PRIMER_RIGHT_3_SEQUENCE=AATATCGCCGATTCCTTCATAG
PRIMER_LEFT_3=1507,22
PRIMER_RIGHT_3=1723,22
PRIMER_LEFT_3_TM=59.685
PRIMER_RIGHT_3_TM=59.466
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=0.9742
PRIMER_LEFT_4_PENALTY=0.895518
PRIMER_RIGHT_4_PENALTY=0.078668
PRIMER_LEFT_4_SEQUENCE=CTTCGAAGCAGAGTTGCAAAAT
PRIMER_RIGHT_4_SEQUENCE=ATATCGCCGATTCCTTCATAGA
PRIMER_LEFT_4=1534,22
PRIMER_RIGHT_4=1722,22
PRIMER_LEFT_4_TM=60.896
PRIMER_RIGHT_4_TM=59.921
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_03355 Ps_contig_mira-and-tgicl-ass_franssen_29680
SEQUENCE=CGTTGTTTTATGTTACGATATTCTGAGGTCAAGAAGATACAAGCTTGTTTTGTTTTGCTTTGTTCAACTGAGTTTCAGAATCACATTTCACAACAGTGATGATGGAATCTTTAACCTCATCTTCTTCTTCAACAACGCTACCTTCTTTCTCCGTCTTCCCTTCAACAACAACAACAAGATCATCCCTTTCTTCCAAGAAAACCCTCCACTTTCGTCTACCCTCCTCCAAACGTGACGATGCCAACGATTCCGACGAGATCAACCTCAACATCAACCTTCCAGTTCCCAGTAACCGCTGTTTCTCCATTTCCCCTCTTTCCAATGATGCTGCGATGGGGCTGGTGTTGAGTGCTGCAACTGGTAGAGGATGGACAACGGGTTCGGGGATGGAAGGGCCGCCTGTTCCTGCTGTTGGGAAGGATGATCAATTTGGGACGGGGAATATTTCAACTTTTCCGTGGTCGCTCTTTACAAAATCTCCGAGGAGGAGGATGCTTATCGCTTTTACTTGTACCATCTGTGGCCAGCGAACTACGCGGGCTATTAATCCTCATGCGTATAATGATGGAACTGTTTTTGTTCAGTGCTGTGGATGCAATGCGTACCATAAGCTTGTGGATCACTTGAACTTGTTTCAGGAGACAAATTGCTATCTGAATTCAAGTTTTAATTATAAAGGTCCTGGATGGGATGATCTTAAGTTCAGGTTCATGGACGTTGATAGCGACAGCGATGATGATATATTCCCAGTTACATGAGTGAATTACCAGCTTATGGAGTATATATTTTCTGCCTCTGATCTTAAGAAATTTTAGATCAGTTGTAATGTATATTGTTTATGTTTTCATGCTTAATAGTAACATTTGTTAGAGGATTGCTTTGTATAGTATATGTGTATGTATAGCTTCGCAACCGAATTGTACGATCATGAATTTAATCATGTACAATACTTTGCATAACTACTTCTTTTGTTGTATGTATAGCTTCACACACTTAACACAATCATCCATAACATATAACATACTAAATATTACAGTTCTTCACAGAAAGGATGATTAAACAACATAAGTCTAAGGCTGTAAGATAATGTGGTTCATAAATACATTACCACATTCCCTCTTCATAATTGCAGAAAATTCTGTGACTTAGCAGTGGTTCACCTGATTACTATTTTCAAAACCAACTTCACTGAGTCATGAGTCATGTTTCTTTTTCTGTAATTCGCCTTTGTGTGTTCTTGGTGTCTCCGTGTGTCAAAGCATGTAGATGTCTTGAGTAGAAGTAGAACCTACACCTAAAGTATGTTTCATTCTATTATTTTGCAACTTATCTCTCACAGGTTATGTACAACACTTTTTGGAGAGGCAAAATGATGAATGAAATATAATGTTTGTTGCTTCTGCGCATAAATAATAGGCAGATTTATCTCCCACCTCAGACCAAGGTGTCTGTGCAACGGTTACTACTAACTTTGTCGCCAACAATAGGAGGCGCAGCACGAATCATAAGACTAAAGGAAAAATGCTGTCATCAGTATTAGCCCAAGGCACATTAGGCTGAATCCTGGAAAATTAAACGGTGCGTCTTCTGACAGGAACAAGGCTGTCAGTGGACTGAAATTAATGGAAGAAATGATGGTTGGCGACTGGAAGTGATTCCNGAGAGACATCCTTAGAACCCTATTTCCCCTGTTCATTTCCGGGTACCTAACTTTTGTTT
TARGET=163,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6005
PRIMER_LEFT_PENALTY=0.501224
PRIMER_RIGHT_PENALTY=0.099308
PRIMER_LEFT_SEQUENCE=TCATCTTCTTCTTCAACAACGC
PRIMER_RIGHT_SEQUENCE=GTTACTGGGAACTGGAAGGTTG
PRIMER_LEFT=116,22
PRIMER_RIGHT=292,22
PRIMER_LEFT_TM=59.499
PRIMER_RIGHT_TM=59.901
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.6007
PRIMER_LEFT_1_PENALTY=0.501224
PRIMER_RIGHT_1_PENALTY=0.099438
PRIMER_LEFT_1_SEQUENCE=TCATCTTCTTCTTCAACAACGC
PRIMER_RIGHT_1_SEQUENCE=ACTGGAAGGTTGATGTTGAGGT
PRIMER_LEFT_1=116,22
PRIMER_RIGHT_1=282,22
PRIMER_LEFT_1_TM=59.499
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.6200
PRIMER_LEFT_2_PENALTY=0.501224
PRIMER_RIGHT_2_PENALTY=0.118777
PRIMER_LEFT_2_SEQUENCE=TCATCTTCTTCTTCAACAACGC
PRIMER_RIGHT_2_SEQUENCE=TGATGTTGAGGTTGATCTCGTC
PRIMER_LEFT_2=116,22
PRIMER_RIGHT_2=272,22
PRIMER_LEFT_2_TM=59.499
PRIMER_RIGHT_2_TM=60.119
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.6372
PRIMER_LEFT_3_PENALTY=0.501224
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=TCATCTTCTTCTTCAACAACGC
PRIMER_RIGHT_3_SEQUENCE=TACTGGGAACTGGAAGGTTGAT
PRIMER_LEFT_3=116,22
PRIMER_RIGHT_3=290,22
PRIMER_LEFT_3_TM=59.499
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.7910
PRIMER_LEFT_4_PENALTY=0.501224
PRIMER_RIGHT_4_PENALTY=0.289736
PRIMER_LEFT_4_SEQUENCE=TCATCTTCTTCTTCAACAACGC
PRIMER_RIGHT_4_SEQUENCE=GAAAGAGGGGAAATGGAGAAAC
PRIMER_LEFT_4=116,22
PRIMER_RIGHT_4=318,22
PRIMER_LEFT_4_TM=59.499
PRIMER_RIGHT_4_TM=60.290
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_03356 Ps_contig_mira-and-tgicl-ass_franssen_29684
SEQUENCE=AAGCATGGTAGTCAACGCAGATACNCGGGGGGTTAAGTGAAACAGCACCACAGAACAGCACCGCCACAACTAAACACTGAAACCCCATCACTTCCAAGTTGAATCTGCAAAACCATGTCGAAGGAAGAAGGAACTCACGCCGTCGTTTCCGATCTGATCAACTTCCTCAACGCATCTCCAACTGCTTTCCACGCCGTCGACGAGGCCAAGAAGCAGTTACAAGCCGCAGGTTATCAACAAATCTCGGAGAAGGAAGTTTGGGAACTGAAAGCTGGTCACAAATACTTCTTCACCAGAAACCATTCCACCATCGTCGCTTTTGCCGTTGGCAAAAGATATGTTGCTGGAAATGGATTCCATATAGTTGGTGCTCACACCGATAGTCCTTCTTTGAAACTGAAACCGGTGTCAAAGGTGGTTAAGGGTGGAATATTGGAGGTTGGTGTTCAAACCTATGGAGGAGGTTTATGGCACACGTGGTTTGATCGGGATTTGACTGTGGCTGGGAGGGTTATCTTAAAAAGAGAAAATGCAGGCTCTGTTTCGTATTCACATCGTCTTGTTAGAATTGAGGAGCCTATAATGCGTGTTCCCACTTTGGCAATTCACTTAGACAGAGGTGTTAATGATGGATTCAAAGTAAACACGCAGACTCATCTTCTTCCCATCCTTGCAACATCTTTAAAGGCAGAGGTTAACAAAGTGTCCTCTGAAAATGGTTCAGTTGAAAGTGGAAAGCAGAATGATGGAAAGAAAGAAAATGATAAAACAGGGTCTAGCAATACAAAGCACCACCCTATACTTTTACAGCTGCTTGCAAGCAAACTTGAGTGTGAACCAGATGACATTTGTGATTTTGAATTGCAAGCTTGTGATACACAACCAAGCTTAATTGCTGGAGCCGCCAAGGAGTTCGTATTTTCAGGACGGCTTGATAACCTATGCATGTCATTTTGCTCATTGAAGGCATTAATTGATGCTACATCCTCTGACAGCAATCTTGAGGAAGAGCCTGGCATTAGAATGGTGGCTTTGTTTGACCATGAGGAATGTGGATCTAACTCTGCTCAAGGAGCAGGATCTCCTGTAGTGCTAGATGCTTTGTCGAGGATTACATATTCCTTCAGCCCAAATTCCAAGCTTCTCGAGAAAGCAGTACAAAAAAGCTTCCTTGTATCTGCTGATATGGCACATGCACTACACCCGAATTACATGGACAGGCATGAAGAAAACCATCAGCCTAAACTACATGGAGGACTTGTCATTAAGCAAAACGCAAACCAACGCTATGCAACCAATGCTGTCACATCCTTCATTTTTAGGGAGATAGCATCAAAACATAATCTTCCTGTTCAGGACTTTGTGGTGCGCAATGACATGGCCTGTGGTTCAACAATCGGTCCTATTCTTGCAAGTGGCATAGGTATTCGAACTGTTGATGTAGGTGCACCTCAGTTGTCAATGCATAGCATCCGTGAAATGTGCGCTGTTGATGATGTGAAATATTCATACGAACATTTCAAGGCCTTTTTCCAGGAATTCTCTCTTCTCGATGCTAATATTGTTGTGGACATCTAGGCTATATATTCACTGAAGATTTACCCATCCATCCTATGCTCTGGGTTTGCTGAGGGATCAGGATTTCTAATGATGTTTTTTTATAATTGCAAAACTGAAATAGTATCTGACTTGTAATTGCAATATTTGGGGCTACTTGCTGTACTTGAATAGTCCACAATTATGCTTCCATTGTAGACCTATTCAGATGAGAAATTAATCTTTCAGAATAGGGGTTCAAATAAAATTTAAATGTAATATGAATTTTCAAGCAGCATATATTACCGAAAATGCAGGACAGTGATGTTAACNTAG
TARGET=749,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0360
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.005674
PRIMER_LEFT_SEQUENCE=TCAGTTGAAAGTGGAAAGCAGA
PRIMER_RIGHT_SEQUENCE=CCTGAAAATACGAACTCCTTGG
PRIMER_LEFT=720,22
PRIMER_RIGHT=925,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.0456
PRIMER_LEFT_1_PENALTY=0.039879
PRIMER_RIGHT_1_PENALTY=0.005674
PRIMER_LEFT_1_SEQUENCE=GGCAGAGGTTAACAAAGTGTCC
PRIMER_RIGHT_1_SEQUENCE=CCTGAAAATACGAACTCCTTGG
PRIMER_LEFT_1=686,22
PRIMER_RIGHT_1=925,22
PRIMER_LEFT_1_TM=60.040
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=0.0676
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=GGCAGAGGTTAACAAAGTGTCC
PRIMER_RIGHT_2_SEQUENCE=GGTGCTTTGTATTGCTAGACCC
PRIMER_LEFT_2=686,22
PRIMER_RIGHT_2=792,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=107
PRIMER_PAIR_PENALTY_3=0.0751
PRIMER_LEFT_3_PENALTY=0.039879
PRIMER_RIGHT_3_PENALTY=0.035219
PRIMER_LEFT_3_SEQUENCE=GGCAGAGGTTAACAAAGTGTCC
PRIMER_RIGHT_3_SEQUENCE=GTTCACACTCAAGTTTGCTTGC
PRIMER_LEFT_3=686,22
PRIMER_RIGHT_3=837,22
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=59.965
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=0.0795
PRIMER_LEFT_4_PENALTY=0.073866
PRIMER_RIGHT_4_PENALTY=0.005674
PRIMER_LEFT_4_SEQUENCE=CAGACTCATCTTCTTCCCATCC
PRIMER_RIGHT_4_SEQUENCE=CCTGAAAATACGAACTCCTTGG
PRIMER_LEFT_4=648,22
PRIMER_RIGHT_4=925,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_03357 Ps_contig_mira-and-tgicl-ass_franssen_29701
SEQUENCE=TCAACGCAGAGTACGTCGGCGACCGCGCCAGATCCCACCACCACCATGGCTTCCATACCTCTCACCACCGCCACCACTGCAACCACCCTCCCATCTCTCCGTCTCCGCTCATCTCCGCCACTTCGCCGACTCCAATCCAACAACGCTTTCAAACCTCTCAAATCAATTCGCCACTCTTCCATTTCCTCCTTCTCACCTCTCCCACTCTCTAACCTCATTTCCAAACGCCAAACACTCACTGTTAAGGCTTCATCAGCCTCCATCACACCAGCTCCATCTCCGGCTCCGGCTCCGGTTCCGGCCGTGCCATGGCAAGGCGCGGCGATAAAACCTCTCTTAGCATCTATAGCAACCGGAGTTATAATCTGGTTTCTTCCCACTCCCGAAGGTGTATCCAGAAACGCATGGCAATTGCTCGCCATTTTCTTAGGAACTATCGTCGGAATCATCACACAGCCATTACCTCTTGGTGCCGTTGCAATTTTGGGATTAGGAGTTTCGGTTCTCACCAAAACCCTACCCTTCGTTGCTGCATTCTCCGGCTTCGGCGATCCAATTCCATGGCTGATTTGTCTTGCATTTTTCTTCGCTAAGGGTTTTATCAAAACCGGACTAGGTAACCGCGTCGCTTATCAATTCGTTTCGCTTTTCGGTAGTTCTTCGTTAGGGTTAGGTTATAGTTTGGTTTTCAGTGAAGCTCTATTGGCACCTGCAATTCCCTCTGTTTCTGCTAGGGCTGGTGGAATTTTTCTTCCATTGGTTAAGTCTCTGTGTGTTGCTTGTGGAAGTAATGTTGGTGATGGAACGGAGAACAAGCTTGGGTCGTGGTTGATGCTTACTTGTTTTCAGACTTCATGTATTTCATCGGCTATGTTTTTGACTGCAATGGCGGCGAATCCGCTGTGTGCAACTCTTACTTTGAATTCAATTAACCAGACAATTGGGTGGTTGGATTGGGCTAAAGCTGCTATTGTGCCTGGTTTGGTGTCGTTGGTTTTGGTGCCGTTGATTTTGTATATTATATACCCTCCTACCATGAAATCTAGTCCTGATGCACCCAAGCTGGCTAGGGAAAGGTTGGCGAAAATGGGGCCAATGTCAGCCAATGAGAAAATCATGACTGCTACTCTGTTTCTTACGGTGGGACTTTGGGTTTTTGGAGGGCTTCTTAATGTAGATGCTGTAACTTCCGCCATTCTTGGATTAGCTGTACTTCTTATCACAGGGGTTGTAACATGGAAGGAGTGCTTAGCTGAGGGTGTTGCCTGGGATACTCTCACGTGGTTTGCTGCCCTCATTGCAATGGCCGGGTATTTGAACAAATACGGTCTTATCTCTTGGTTCAGTCAAACTGTAGTCAAGTTTGTTGGTGGATTGGGCCTTTCATGGCAACTATCTTTTGGTATTTTGGTTCTCCTATACTTCTACTCCCATTACTTCTTTGCCAGTGGAGCTGCTCATATTGGCGCCATGTTTACTGCATTTTTGTCTGTTGCTACTGCTCTGGGGACTCCACCATTCTTCGGAGCCATGGTGCTGTCTTTCCTCTCCAACCTTATGGGCGGCCTTACTCATTATGGGATTGGGTCAGCTCCAGTGTTTTTCGGTGCCAACTATGTCCCGCTTGCCAAATGGTGGGGTTACGGCTTTGTTATTAGTATTGTTAACATTATAATCTGGCTTGGACTTGGGTCTATTTGGTGGAAAGCCATTGGCTTGTGGTAAGGGGTCTGACATAGAATTTTGCCAAATACAGTTTAAGATTTTTGCATACATCCATGTACGCATTCTTGTTGTTATCAGCAGGTGAAGTAAATTGCGATTTTTTAACCTATCCTTTCTAGAAATAATCTTTGGAAGGTTTTGTTGTAGATTTAAACGGGAGCCTGGATACCGATGATCGAGTTAGATCTTGGGTAGACCAGTTACATTATAATTTATGAGATGTAAAAACACCAAGTTATTCAGGATATATTGGCATGACTCATATGCTTCCAATCTTTTCTTTCCTTTAAATTTTTGGTGCAAGTGTTTATTTTCTTTTATAATGGGATGGCAATAGTGTCTGTGTAATTAA
TARGET=35,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03358 Ps_contig_mira-and-tgicl-ass_franssen_29713
SEQUENCE=GTAAGCGATAACAAAAAATTATTATTATTATTTTAAATTAACACTCGATATTTTAAGAGAATTGAAAAAGTACAGATTTATTTTCATGTGGTTCAGACCATAGTAACTTAGCAATACAATTTTTATTGCCATGGTAACCTTTTGGATATTTATAATAAAAGACTCTAAGTCCAAATTCCAATTAAGGTAGTTTTTGTTTTTCTAAGGGCACTTGCGTTTGCCACCATGAGTAGTCAAGCTGGCATAACAGGGACACTTTTCTTGGTTTCCAGCCGTGCCAGGTGGCACGCAATTACATCTTCTGCAACAAGTCCCACATGCTCTTTTACATAATCTTGGTCGAGATGATAAACGGCACCTCGCAACACATGATCCATTACAATCTATTTTCTGAAGAAGAGAGCCTTCTGCTTCTGTATACTCAGATTGATGGGCTGCATCAACAAGATAGAAAAAAGAGAAGTGATGCAAGAAGAGAAGCAGCTAGAAGCTTAGAGAAAGCCATGATTGAGAATGGAAATTAAGTGTCAGCCCCGANAGATATAATGAGGTAATATTTATATGTGATGAAAAGAATATGAACAAAATTTGCTAGCTGGAGTACTGCTTTTTTTAGACTCAAGTCCCCC
TARGET=18,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03359 Ps_contig_mira-and-tgicl-ass_franssen_29715
SEQUENCE=TGGCAGGCCGATTGCCGTGGACTCTGGGGCYCGAACTATACACTATTTTAGAAATGTGTCGTGCATTTGACAAAGTATTTAAAGAGCACCTGGATGGAGGGCGTCCTGGCGGGGACCGGATATACGGAGTTTTTGATCACCAATTACCGGCTGCTTTGAAAAAGCTACCCTTTGATCGCCATCTTTCCTTAAAAAATGTCCAAAAAAATTGTCACTGAAGCTGATGGGTATCAACCTCATCTGATTGCTCCAGAGCAGGGTTATAGAAGACTCATTGAAGGGTCTATCAGCTATTTCAAAGGCCCAGCTGAAGCCTCTGTGGATGCCGTCCTACTTTTAGTTTTAAAGGTAACTTGTACGAAAGTCAGTTGCAGAAACTGAGGAGTTAAGAAGATTCCCAACGCTTTCACATGATATTACAACTGCTGCAAATGAAGCTCTGGATAGATTCCGAGACGAAAGCAAGAAGACAGTTTTGCGGTTGGTTGATATGGAGTCTAGCTATCTAACTGCAGAGTTTTTTAGAAAGATTCATCTCGAACCAGAAAAGAACCCAAATGGACCACCAAATTCAAACCGGAATGCTCCCCCCAACACAGACAATTTTACCGACAATCACCTCCGCAAGATTGGATCAAATGTAAGTGCTTATATCAGCATGGTATGTGATACACTTAAGAATACTATACCAAAAGCGGTAGTCTATTGCCAAGTCAGAGAAGCCAAGAGATCATTGTTAAACTACTTCTACATTCAAGTCGGAAAGAAGGAGAAGGATAGATTGGGCGCTATGTTGGATGAAGACCCAGCCCTAATGGAAAGGAGAAATCAAATAGCTAAAAGACTCGAATTGTACAAGCAAGCCAGAGACGATATTGACTCTGTGGCTTGGAAATAGGGAAGATCCCACATTTTACACGAGGGAAGATCCCACATATTATAACAGATACTAGATACTGCGAGGAAGATTTGCCACATCTTGTTTTTATATTTTTCCAATCAAACTCAAACAGAATGGTCTTCGAATGGTCTACGTTACAAGAAATTGATTTGCACATGCCTATATTCTTTTGGCCCAGTGTATATTGCCACTGAGTTCTGCTTTGTGATTTTATGTAACTAAAGAGTCGTTGGTTGTTGGATTTTTTCGCAACCATGTTGATTATTATTATTACTACGAAAGATTATTATTGTTATTTGGACTGAATGTTGATTCTTTTAACAGAGCTTATCAATTAAAANNCTAAAGAAAAAGT
TARGET=1162,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03360 Ps_contig_mira-and-tgicl-ass_franssen_29717
SEQUENCE=AAGCAGTAGTATCAARYGCGGGGTACTGCGTTTGTGCCTCCAGCTGCTGCGATTGAAGAGAGGATTCAGCAGAGAATTCATCTTCGCAATCATGGGTAAGGTTCATGGATCACTCGCTCGTGCCGGTAAGGTGAGAGGCCAAACACCAAAAGTGGCCAAACAGGATAAGAAGAAGAAGCCCCGTGGTCGTGCTCACAAGCGCATGCAGTACAATCGCAGATTCGTCACTGCTGTGGTTGGATTTGGCAAGAAGAGAGGACCCAACTCATCTGAGAAGTAAGGTTGAGGAGAAGAATGTGTAATTCTTTAGGTTTGAAAATGTGTTAAGAACTTCAATTTGATTTTCCTTTTTATTAATTTTGGGATATAGTAAGTTTATTATTATCTATGAATGGGTTTTGTGCTAGCTATATGTCAAACTTTATATTTTGCCTATGTAATTCACAATATTCTAAGTTTTGTTGACCCAATTTTTCATTTGGCCTCAAGAATTTATACTTTTGTTATTTCAGTGTGTTGTTGTTGTTGTTGTATCTTTTAAATCATAGTTACAAGTAGANNAATGTTAAATAA
TARGET=167,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03361 Ps_contig_mira-and-tgicl-ass_franssen_29717
SEQUENCE=AAGCAGTAGTATCAARYGCGGGGTACTGCGTTTGTGCCTCCAGCTGCTGCGATTGAAGAGAGGATTCAGCAGAGAATTCATCTTCGCAATCATGGGTAAGGTTCATGGATCACTCGCTCGTGCCGGTAAGGTGAGAGGCCAAACACCAAAAGTGGCCAAACAGGATAAGAAGAAGAAGCCCCGTGGTCGTGCTCACAAGCGCATGCAGTACAATCGCAGATTCGTCACTGCTGTGGTTGGATTTGGCAAGAAGAGAGGACCCAACTCATCTGAGAAGTAAGGTTGAGGAGAAGAATGTGTAATTCTTTAGGTTTGAAAATGTGTTAAGAACTTCAATTTGATTTTCCTTTTTATTAATTTTGGGATATAGTAAGTTTATTATTATCTATGAATGGGTTTTGTGCTAGCTATATGTCAAACTTTATATTTTGCCTATGTAATTCACAATATTCTAAGTTTTGTTGACCCAATTTTTCATTTGGCCTCAAGAATTTATACTTTTGTTATTTCAGTGTGTTGTTGTTGTTGTTGTATCTTTTAAATCATAGTTACAAGTAGANNAATGTTAAATAA
TARGET=515,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03362 Ps_contig_mira-and-tgicl-ass_franssen_29722
SEQUENCE=CCCTAACATTTTGCAAGTAATTCTTAAAATATATGAAAATATGTCTCATTAGTAATTCAACTAAATTCTCACAAAGCATTCCAAATTGAATATGAAATCATATACTTGAGTGTCTAAAAGCATAGCCTAAGGAATATTCATAAGATACAATCTAATTAACACATTCATAAACATAACTGCATACAGTACAATTTTAAAGGATCAAAAGCAGCTGCAAATTCTATAGAGAGCTGCAATATGTGCACATCAAGTAACTGGTGGGCGAGGCAGTCTTTTGGCAATTTCCTCAAACAGTTCATTGATATTATCCGCTGTCTTTGCCGAGGTCTCTATAAAAAACATTCCGTTCTTTTCTGAATAGTCCATGCCATCCTGAACAGCCACTTCCCGCTTCTCGTGAAGATCAGCTTTATTACCAACCAATGCCATCACTATATCAGGGCTTCCATGTTTTTGTAGCTCCTTAACCCAGTACTGTGCTTTGCTGAAAGATTCCGGGCTTGTTATATCATAGACAATAACTGCAACCGATGCACCACGATAATACAGCGGTGCAAGTGCAGCATACCTCTCTTGACCAGCGGTATCCCATATTTCAAACTTGACTGTTGTAGAGTCTTGAAGAGCGATTGTTTGTGACAAGAAAGATGCTCCAACAGTTACCTTGGATGTTGGATCAAACTGACCACGGACAAATCGCAGAACAATACAACTCTTACCAACACCAGAATCACCTAAGAGAACGAGCTTAACACGAAGATTCTTAGCGTCTTGTCCTCCACCATTCTCTGAATTAGGTCGACCTAGCTGCCCTGATTCCCTATCTGGAAAAGAGGAGCCACAACCCATGTCGAGAGAATAAGGGATGATGGGTGATGCTGCACAACACAACACACCACTGGTATAAAAAATTGGAACTTTTTTTTGTTGTTGTTGGTTTGTTGTGAGAGAAATTGGTGGGGTGAGAAGGATTCAGAAAATCTGGGGAAATTGAGGGTTCGTTAACGGATGGATGCTTGGATTGAATGGAATAGAATCCTATATCGTGCTTTCCGTGAGGACGAGGATTCCACCTCTCGTCAAACTTACAAAAGGAAT
TARGET=925,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0114
PRIMER_LEFT_PENALTY=0.003166
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=GGACAAATCGCAGAACAATACA
PRIMER_RIGHT_SEQUENCE=CCACCAATTTCTCTCACAACAA
PRIMER_LEFT=689,22
PRIMER_RIGHT=959,22
PRIMER_LEFT_TM=60.003
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.0375
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.008257
PRIMER_LEFT_1_SEQUENCE=CTTGGATGTTGGATCAAACTGA
PRIMER_RIGHT_1_SEQUENCE=CCACCAATTTCTCTCACAACAA
PRIMER_LEFT_1=663,22
PRIMER_RIGHT_1=959,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=60.008
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.0510
PRIMER_LEFT_2_PENALTY=0.006945
PRIMER_RIGHT_2_PENALTY=0.044097
PRIMER_LEFT_2_SEQUENCE=CACAACCCATGTCGAGAGAATA
PRIMER_RIGHT_2_SEQUENCE=CGGAAAGCACGATATAGGATTC
PRIMER_LEFT_2=839,22
PRIMER_RIGHT_2=1054,22
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=59.956
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=216
PRIMER_PAIR_PENALTY_3=0.1606
PRIMER_LEFT_3_PENALTY=0.152322
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=GAGAATAAGGGATGATGGGTGA
PRIMER_RIGHT_3_SEQUENCE=CCACCAATTTCTCTCACAACAA
PRIMER_LEFT_3=854,22
PRIMER_RIGHT_3=959,22
PRIMER_LEFT_3_TM=60.152
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.1964
PRIMER_LEFT_4_PENALTY=0.152322
PRIMER_RIGHT_4_PENALTY=0.044097
PRIMER_LEFT_4_SEQUENCE=GAGAATAAGGGATGATGGGTGA
PRIMER_RIGHT_4_SEQUENCE=CGGAAAGCACGATATAGGATTC
PRIMER_LEFT_4=854,22
PRIMER_RIGHT_4=1054,22
PRIMER_LEFT_4_TM=60.152
PRIMER_RIGHT_4_TM=59.956
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=201
=
PRIMER_SEQUENCE_ID=SSR_03363 Ps_contig_mira-and-tgicl-ass_franssen_29732
SEQUENCE=CCGTAAAGGAACCGAACGAGGTAGTTCTAACGACCACGAGTAACGCGGGACGGTACATNTACACCAACAACCTCTCTCTCTCTCTCTCCGCATATAACTCCTCCTGCTATCATCCACATAGACACAACAACCACCCTGTGAAGATGGTAGGAGCTATTAAGGCTGCACTCGGAGACGCAGTATTGACTTTCATGTGGGTTTTTTCTTCTTCCATGTTGGGTATAGTTTCAAAGGCAATAACGCAAGCACTTGGTCTTCAAGATGTTTCGTACAATGGTTTCCCTTACCCTTCTTTGATTATCATCACTACCCTTGTGTTTCTTCTCGTTTTTCTTTTCACCTTAATTGGTAGTGCAATGGGTGGTGCTAGCTTTAACCCTACTGGTACTGCTGCTTTTTATTCCGTTGGTTTGGGTTCTGATACGCTTTTTTCCATGGCTCTTCGTTTCCCTGCCCAGGCTCTTGGTGCTGCGGGTGGTGCAATGGCAATTGCAGAGTTGATTAATCCGAAATATAAACACATGATTGGGGGTCCTTCTTTGAAAGTGGACTTGCATACTGGGGCTGTTGCTGAGTTGGTTTTAACATTTGTGATTACTTTTCTGGTCCTATGCATTTTCATCAAGGGGCCTCGTAATGATTTATTGAAGATTTGGTTGCTGGCCATGTCAACCGTCACTTTGGTCATGGTTGGCTCTGCTTACACTGGTCCGTCCATGAACCCTGCCAATGCCTTTGGTTGGGCATACTTAAACAACCGGCACAACACATGGGACCAATTCTATGTCTACTGGATTTGCCCCTTCACTGGCGCAATATTGGCTGCTTGGCTGTTCCGGGCTATCTTCCCTCCACCAGAAGTAAAACAGAAGAAAGCATGATAGCTTTGTTTCTTATATCAGTTTGTTAAATGTCATGCATCATGTTTCAGCTCATAATGGAACATGTTTCATTAAGTATGATATCAAATTTGAAATTGTTAGTGCTAAATTATTGGAGTTATTGATAAATGGATAACTCATAGATTCAGTTTCTAATTGGAAAAGTTCAATAAAGATATAAGCAGTATCTAGGCTGGGTATTGATTTA
TARGET=72,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0667
PRIMER_LEFT_PENALTY=0.053256
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=GGGACGGTACATNTACACCAAC
PRIMER_RIGHT_SEQUENCE=GGGAAACCATTGTACGAAACAT
PRIMER_LEFT=46,22
PRIMER_RIGHT=282,22
PRIMER_LEFT_TM=60.053
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.50
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.50
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.1488
PRIMER_LEFT_1_PENALTY=0.053256
PRIMER_RIGHT_1_PENALTY=0.095566
PRIMER_LEFT_1_SEQUENCE=GGGACGGTACATNTACACCAAC
PRIMER_RIGHT_1_SEQUENCE=TGCAGCCTTAATAGCTCCTACC
PRIMER_LEFT_1=46,22
PRIMER_RIGHT_1=166,22
PRIMER_LEFT_1_TM=60.053
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.50
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.50
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=121
PRIMER_PAIR_PENALTY_2=0.1488
PRIMER_LEFT_2_PENALTY=0.053256
PRIMER_RIGHT_2_PENALTY=0.095566
PRIMER_LEFT_2_SEQUENCE=GGGACGGTACATNTACACCAAC
PRIMER_RIGHT_2_SEQUENCE=GCAGCCTTAATAGCTCCTACCA
PRIMER_LEFT_2=46,22
PRIMER_RIGHT_2=165,22
PRIMER_LEFT_2_TM=60.053
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.50
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.50
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=0.3020
PRIMER_LEFT_3_PENALTY=0.288644
PRIMER_RIGHT_3_PENALTY=0.013401
PRIMER_LEFT_3_SEQUENCE=ACCGAACGAGGTAGTTCTAACG
PRIMER_RIGHT_3_SEQUENCE=GGGAAACCATTGTACGAAACAT
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=282,22
PRIMER_LEFT_3_TM=59.711
PRIMER_RIGHT_3_TM=59.987
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.3343
PRIMER_LEFT_4_PENALTY=0.053256
PRIMER_RIGHT_4_PENALTY=0.281044
PRIMER_LEFT_4_SEQUENCE=GGGACGGTACATNTACACCAAC
PRIMER_RIGHT_4_SEQUENCE=CTACCATCTTCACAGGGTGGTT
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=149,22
PRIMER_LEFT_4_TM=60.053
PRIMER_RIGHT_4_TM=60.281
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.50
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.50
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=104
=
PRIMER_SEQUENCE_ID=SSR_03364 Ps_contig_mira-and-tgicl-ass_franssen_29733
SEQUENCE=GGGTAAAATTGATATGCTATTTTATTAAATTAACACAACTTTTGGATTACTCCAATTTTTATTACAAACACTGATGATGATTCTAAATTAGAGAATCCTGTAATCAAAACTTTGCATATTCTGTAGGTATGAGAACTCAACTAACATATAAGTATACACATCTTTCTCTTTACATTATTAACACCTCTAATACATATACAGATTTTCCACAAAAATCAGTCATGGAGGAAATTTCAAAATCTAGTCGGAATTTCTTTCTTCTGATTTCAGCTGTTGGATTCTTTGTCTTAGTTTTCGCCGGTGATCGTGTATCTTCCAAAGGCCATGTCCCAATATAACAGTTGAGTAAATGCCATGTGTGATAATCGGAGAAAGAATATTATTTGTTTGAATCCACTCGAAACCGAGGTAGAGCGCCAATAGTCCTTCCAGAAGTGGTGAATAGATTTTCCTCATTTGCCTTTTCTCATACCAAGCTTCAAAGAGCTTTTTCAAATCTTGACGACTGGCGCGAGATTGTAAAACTGGTGCAACAATATAGGTAGGATCTTTGGGAGAGGCAGCGACGTAGTAGAGAGAACCAGTAAGGATAGCGGTAAGTACAGCTGCAAGTGCTTGAGCAAATGGAACAAACGGCGGCAGAACTCCTGTCAAAGACGCCATTCCTCGAACATCTTGCATAACATTACTGCCTCGTAAAAATATATCAGCAAGTGCCCCCTGAACTGCAGCTCTATAGAAGAGTTCCTCTCCCACTGAACTTGCAGCAACCATCAGTATAAACTGCAAAGGAGACATGCCATAGAAAAAACTCCATAGCTCTTCATCTTCTACGTCTCTAATGGCACGAGCATGGGGTGATAGCTTCACAACTTCATCATCAAGTATAAAAAGAAGAGCCATAATTGGAGGAACTGCATATCCAAGTCCTTCAAGAACAGCATCAATTGAAAGATGAAAACCTCCCAGAGAGTCGATTCCGGTAATCGAACATATGAAACTCCCCGCAACAGCCATCGCGCCATAAATCCCGATGCCATAGCTGAGTCTCACAACAGCACCAATTTTTTCCCAAACAGGAAAGTCAGCAGCATCATTGAAACCTTGGTTGAATGTGGTGACCTCCATAGCAGCCGGTGCAGTGAACCCGGTTCGAGTCTCAGCTCTGTCATCGATTGTTTCACCGGTTCGTTCAGCAGAAGCTTTAACACACCGAACAGAAACTACTCTTGAAGCATCAAAGAAAGAAACCCTTTTCTTCCTTTGATGAAACTCACATAGCTTGTTAGAGAAGTGAACAAGTGAAGATGAAGAAGGGTTTGGTGTTAGTGTAAGAGAAAGCTCCATTCAATAGAGAGAGAGAGAGAGAATTCAAAATGGATTTGTGTTGTTGTTGATGTTGTTGTTCATGTTGAGTTTGTGTTCAAAGAACCCCC
TARGET=1353,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8982
PRIMER_LEFT_PENALTY=0.019767
PRIMER_RIGHT_PENALTY=0.878426
PRIMER_LEFT_SEQUENCE=TCTCAGCTCTGTCATCGATTGT
PRIMER_RIGHT_SEQUENCE=GGGTTCTTTGAACACAAACTCA
PRIMER_LEFT=1157,22
PRIMER_RIGHT=1433,22
PRIMER_LEFT_TM=60.020
PRIMER_RIGHT_TM=59.122
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=277
PRIMER_PAIR_PENALTY_1=0.9020
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.878426
PRIMER_LEFT_1_SEQUENCE=GTGAAGATGAAGAAGGGTTTGG
PRIMER_RIGHT_1_SEQUENCE=GGGTTCTTTGAACACAAACTCA
PRIMER_LEFT_1=1301,22
PRIMER_RIGHT_1=1433,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.122
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=0.9965
PRIMER_LEFT_2_PENALTY=0.118120
PRIMER_RIGHT_2_PENALTY=0.878426
PRIMER_LEFT_2_SEQUENCE=TCAGCTCTGTCATCGATTGTTT
PRIMER_RIGHT_2_SEQUENCE=GGGTTCTTTGAACACAAACTCA
PRIMER_LEFT_2=1159,22
PRIMER_RIGHT_2=1433,22
PRIMER_LEFT_2_TM=59.882
PRIMER_RIGHT_2_TM=59.122
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.9965
PRIMER_LEFT_3_PENALTY=0.118120
PRIMER_RIGHT_3_PENALTY=0.878426
PRIMER_LEFT_3_SEQUENCE=CAGCTCTGTCATCGATTGTTTC
PRIMER_RIGHT_3_SEQUENCE=GGGTTCTTTGAACACAAACTCA
PRIMER_LEFT_3=1160,22
PRIMER_RIGHT_3=1433,22
PRIMER_LEFT_3_TM=59.882
PRIMER_RIGHT_3_TM=59.122
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=1.0647
PRIMER_LEFT_4_PENALTY=0.186309
PRIMER_RIGHT_4_PENALTY=0.878426
PRIMER_LEFT_4_SEQUENCE=TCGTTCAGCAGAAGCTTTAACA
PRIMER_RIGHT_4_SEQUENCE=GGGTTCTTTGAACACAAACTCA
PRIMER_LEFT_4=1188,22
PRIMER_RIGHT_4=1433,22
PRIMER_LEFT_4_TM=60.186
PRIMER_RIGHT_4_TM=59.122
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_03365 Ps_contig_mira-and-tgicl-ass_franssen_29749
SEQUENCE=AACTTTTTAAAATTACCAAATAGCAACAGCAATATAAGATACACTTTTTGGCTTTTTTTCTTGCATAGGAATAACTTTCATCTTGCTTTTAGCCTTTTAGGCTGAGGAGAGTGATATGAGTTATGGTTGGATAAAACAGTTTCATGATATGAAACTTAATAAGAGAAGTGGATTATCATGATTATCAAGGAGGAAGCAAAAGTCAATAGCAGCTGCAGTGACTGTGACTGTATGATGAATAGGAAGAGTCTATGTACAACTGGAAATTAAAATGAAATATAGGTAACAAAGGAATAACACTATTATGCAGTTGCAGCAGCTTGTTCTTCAACGGCCTGTGATGCTGGGAGAAGGGTGCAAACCAGGTTGCGGTCACCATACACATTATCAACACGTCCTGTGGTAGGCCAAAACTTTGCACCACGGAGCCATGCAGCTGGGAAGGCTGCATACTCCCTGGAGTATGGTTTGGTCCATGCATCAGCCATGAGCAGTGATGGTGGATGAGGGGCTCCCTTGAGAACATTGTTGTGAACATCAGCATTTCCTTTCTCAACCTCAGCAATTTCCTTTCTAATGGAAATAAGAGCATCGCAAAACCTGTCTAACTCAGCCTTGCTTTCACTTTCAGTAGGCTCAATCATGAGTGTACCAGCGACAGGCCATGACATTGTTGGTCCATGAAATCCATAGTCCATGAGACGTTTCGCAACATCTTCAGGCTCTATTCCAGCAGTATTCTTAAATCCTCTTAAGTCAATAATGAATTCATGAGCAACTGTTCCATTGACTCCGCGGAAAAGAACTGGGTAATAACTCTCCAATCGTTTCGCCATGTAGTTTGCATTCAAAATGGCTATCTTTGACGCGTCAGTGAGTCCTTGAGAACCCATCATGGCTATGTATGTGTAAGAGATTGGCAATATGAGTGCTGATCCCCAAGGTGCAGCTGAGATACTACCAAGTGGTTGAGGATTCTCAGGTGCAGGAATTCCACCGGTTGGCACCACGGGGTGTGAGGGTAAAAATGGTGCCAGGTGTTTCTTTACACCAATAGGACCCATGCCAGGGCCACCTCCTCCATGAGGAATGCAAAATGTCTTATGGAGATTGAGATGGCAAACATCTGCTCCAATCCAACCTGGGCTTGTAAGTCCTACCTGTGCATTCATGTTGGCACCATCCATGTATACTTGGCCACCATTATCATGGATAATCTTGCAAATATCATCAATACCTTCTTCATAAACACCATGAGTTGAAGGATATGTTACCATAAATGCAGATAAGTTGTCCTTATGTTTTTCAGCAGCCTTCTTCAACTCTTCAATGTTAATGTTTCCCTTAGCATCAGTTCCTATGGTAACAATTTTCATTCCAACCATAGCAGCACTAGCAGGATTTGTACCATGTGCTGATGCTGGTATAATGCAAACATTGCGGTGGTGGTCACCTCTTGACAAATGATATGCACGAATAACCATGAGTCCGGCATATTCTCCGGCAGCACCGGCATTTGGTTGCAAAGAGAAGGAGTCAAATCCAGTGATGGTACACAACAGGTCACCCAAATTGTTGAACATTTCCTGATACCCTTGAGCCTGTTCAGTTGGTGCGAAAGGGTGCAAATCGGTAAAGCTAGGCCATGTCACGGGCATCATTTCAGTTGTTGCATTCAGCTTCATTGTACAAGATCCAAGTGGGATCATACTGTGGCAAAGTGAGAGATCTTTTGATTGTAACCTATGAATGTATCTCAGCAACTCATGCTCAGTTTGGTACGTGTTAAAGATAGGGTGTGTCAAATAGGGACTCTCCCTAACTAATCCAGAAGGAATTGCATTTTGAAACTCTGGTGCAAGAGATGCCGCTGTGAATGAGACAGGCTTGCCACCAGCGAAAACTTTGAAAAGCTTATCAACATCCTCTAACGTGGTTGTTTCGTCAAAAGCAGCGGTGATAGTGTTTCCATCTACAACACGCAAATTGATTTCACTTTTGATAGCAGCATCTGCAATTGCTTTGGCATTTGAAGTCTTAACCTTCACAGTGTCAAAGAAGCCAAGATCCTGAACTTCCAATCCAAGTTTCTTTAATCCAAGAGCAAACACACCAGCAAGACCATGAACCCTTTGTGCAATGGCTTTAAGGCCTTCAGGTCCATGATATACAGCATACATAGCAGCCATGTTTGCAAGCAGTGCCTGAGCAGTGCAAATGTTGCTGGTGGCCTTGTCCCTACGGATATGCTGCTCCCTAGTTTGCATAGCCATCCTCAAAGCTTGCTTTCCAGAAGAATCAACACTAACACCGATGATTCTTCCAGGCATCATCCTCTTATACTCTTGCGAAGTAGCCAAGAAAGCAGCATGAGGACCACCATAACCCATTGGAACACAAAATCTCTGAGCTGAACCAACAACAATATCAGCTCCAAACTCACCCGGAGGTTTCAACACAGTCAATGCCAACAGATCACTCGCCATAACAACCTTAACCTCGTTAGCATGAGCCTTCTTAATAAATTCACCGTAATCCAAAACCTCACCTTCAGTACCAGGATACTGAACAAGAACACCACAAACATCACCAGATTTGTAATCAATATCCTTAAGATCTTTCACCACAACCTTGAGTTCAAATCCATCGGCTCTCGTCTGACATATATCAATGGTTTGTGGATGACAATTACTAGCAATAATGAAAGTTTTCTTCTTACCCTTTTGAATATTATTACACATAGACATTGCTTCCGCAGCAGCAGTACCTTCATCAAGCAATGAAGCATTTGACATAGGCAAACCAGTGAGATCAGTAATCATGGTCTGAAAATTCAACAAAGATTCAAGACGGCCTTGAGATATCTCAGCTTGATAAGGTGTATACTGTGTATACCAAGCAGGATTCTCCATGATGTTCCTCAAAATCACAGGTGGAACATGAGTGTTATAGTATCCCATACCAATAAACGATTTGAAAACCTTGTTTTTCGAAGCTAAATCCTTCATGTGCTCAATCATTTGACCTTCTGTCAATCCACCATCAAATTTATTGAACTTCATTTCCTTCAACCGAATGGATTTCGGCACGGTTGCATCAACGAGTGAATCAAGCGTATCGAAACCAACCGATTCAGCCATTTTCGTTTGTTCATCCGGTGTTGCTGAGTTATGGCGTCTCGGAAATGTGTCACTCGGTTTCAACGCTTCAACGGAGATGGATCGAGATTGTGATGGATAACCAACACCAAGAAACCCACCAACACGTCGTGAAACATTGTTGTCTGGTTTGGATCCTCTGCCTCTAAGAATAGAGTTTGAAACAGAGGAAACGTACCTTGAAGATGAACCAGAGAGTGAAAAAGGAAGCGGCGTTGTTGTTGTTGTTGATGTTGATTCAGTCTTCCGGTTCTGTTTCGCCTCTGAAAGAAGACGCTTCAGAGTTGCACGGTTTGCGAGTCTACGAGCACGTTCCATGAATGCTGTTACAGAGAAAGAAAAAAACCAGAGTAAAATGTTTTTGATTTGGTTAAGGAAATTCTAGAATATGTTTTTGGTTTATGATGATTCCATTGAAGTGTGTGTCCGCGTACTCTGCGTTGATTACCTACTTGTCTTT
TARGET=3373,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1939
PRIMER_LEFT_PENALTY=0.064105
PRIMER_RIGHT_PENALTY=0.129818
PRIMER_LEFT_SEQUENCE=CGTGAAACATTGTTGTCTGGTT
PRIMER_RIGHT_SEQUENCE=AACCGGAAGACTGAATCAACAT
PRIMER_LEFT=3267,22
PRIMER_RIGHT=3409,22
PRIMER_LEFT_TM=59.936
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=143
PRIMER_PAIR_PENALTY_1=0.2076
PRIMER_LEFT_1_PENALTY=0.015253
PRIMER_RIGHT_1_PENALTY=0.192310
PRIMER_LEFT_1_SEQUENCE=GAAATGTGTCACTCGGTTTCAA
PRIMER_RIGHT_1_SEQUENCE=GCAACTCTGAAGCGTCTTCTTT
PRIMER_LEFT_1=3181,22
PRIMER_RIGHT_1=3445,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=60.192
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.2564
PRIMER_LEFT_2_PENALTY=0.064105
PRIMER_RIGHT_2_PENALTY=0.192310
PRIMER_LEFT_2_SEQUENCE=CGTGAAACATTGTTGTCTGGTT
PRIMER_RIGHT_2_SEQUENCE=GCAACTCTGAAGCGTCTTCTTT
PRIMER_LEFT_2=3267,22
PRIMER_RIGHT_2=3445,22
PRIMER_LEFT_2_TM=59.936
PRIMER_RIGHT_2_TM=60.192
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.3572
PRIMER_LEFT_3_PENALTY=0.227356
PRIMER_RIGHT_3_PENALTY=0.129818
PRIMER_LEFT_3_SEQUENCE=TGATGGATAACCAACACCAAGA
PRIMER_RIGHT_3_SEQUENCE=AACCGGAAGACTGAATCAACAT
PRIMER_LEFT_3=3230,22
PRIMER_RIGHT_3=3409,22
PRIMER_LEFT_3_TM=60.227
PRIMER_RIGHT_3_TM=59.870
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.3942
PRIMER_LEFT_4_PENALTY=0.015253
PRIMER_RIGHT_4_PENALTY=0.378922
PRIMER_LEFT_4_SEQUENCE=GAAATGTGTCACTCGGTTTCAA
PRIMER_RIGHT_4_SEQUENCE=AGAACCGGAAGACTGAATCAAC
PRIMER_LEFT_4=3181,22
PRIMER_RIGHT_4=3411,22
PRIMER_LEFT_4_TM=60.015
PRIMER_RIGHT_4_TM=59.621
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_03366 Ps_contig_mira-and-tgicl-ass_franssen_29753
SEQUENCE=GTCGGGGTAATGTCTTAAAAAAGTGTGTTTGGAACAAGCCTTTAAATTATATGACTCATATGCCACAACNCTAAAAAGAGGGGGAAATACATTGTATTTCATTTCTCACGCAAGCACTGTCTTGTTTGTTGTTGTGACCTATACTTCATAACACAACACATTCCTACTACAACTCATTCATATTATAAAGAAAGATCACAACTTTGCGAGAGAGAGAGAGAGAAAGCCTACGTGGGTTCTTCTTCAATTTCAAGAAACATGGATGTGGGTCAGATGCGGAGACAATGGATGGACTACATCAAAACCATGTTCGAGGAGGGGTTCTTAGATGGTCAGTTTCTGCAACTTCAACAGCTACAAGATGAGAATAACCCTGAGTTTGTTTTTGAAGTTGTTTCTCTTTTCTTTGATGATTCCGAGAGGATTCTCAAAGATCTGTCCTTTGCTCTGGAGCAGCAAAGTGTTGACTTCAAAAAAGTTGATGCCCATGTGCACCAGTTTAAGGGTAGCAGTGCAAGCATAGGTGCACAAGGGGTGAAAAATGCCTGCATTGCTTTCCGCAACTTCTGCGAGGAACAAAACATAGATGCTTGCCGCAGATGTTTACAGCAAGTGAAGCAAGAGTACTTTGTTGTTAAGAATAAGCTTGAAACATTGTTGAGGCTTGAGCAGCAGATAGTTGCAGCTGGCGGCTCGATCCCTACGATGGAACTGAGTTTTTAAAGTGAGTGATATGTAAAAATGCTTGTATTATGATCCTCTTGTCTGTCGAGGATCATCTATTTCTCTTATGTCTCTTGTGAATGTATCAATTTTTATGTGGATAGAAGTTTTCATCCAATGTACCTTGTTCTTCACAAGAAAACACTTTCATAATCTGTAATATTTTTCATGCTTTAATGAAGGTTAATGGCGTTAGTATAAGATAAATTAGCTTTCC
TARGET=208,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0917
PRIMER_LEFT_PENALTY=0.059210
PRIMER_RIGHT_PENALTY=0.032476
PRIMER_LEFT_SEQUENCE=ATGCCACAACNCTAAAAAGAGG
PRIMER_RIGHT_SEQUENCE=ATCTAAGAACCCCTCCTCGAAC
PRIMER_LEFT=59,22
PRIMER_RIGHT=329,22
PRIMER_LEFT_TM=59.941
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.1983
PRIMER_LEFT_1_PENALTY=0.059210
PRIMER_RIGHT_1_PENALTY=0.139129
PRIMER_LEFT_1_SEQUENCE=ATGCCACAACNCTAAAAAGAGG
PRIMER_RIGHT_1_SEQUENCE=CCTCGAACATGGTTTTGATGTA
PRIMER_LEFT_1=59,22
PRIMER_RIGHT_1=315,22
PRIMER_LEFT_1_TM=59.941
PRIMER_RIGHT_1_TM=59.861
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.2139
PRIMER_LEFT_2_PENALTY=0.074807
PRIMER_RIGHT_2_PENALTY=0.139129
PRIMER_LEFT_2_SEQUENCE=AGTGTGTTTGGAACAAGCCTTT
PRIMER_RIGHT_2_SEQUENCE=CCTCGAACATGGTTTTGATGTA
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=315,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=59.861
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.2139
PRIMER_LEFT_3_PENALTY=0.074807
PRIMER_RIGHT_3_PENALTY=0.139129
PRIMER_LEFT_3_SEQUENCE=AAAGTGTGTTTGGAACAAGCCT
PRIMER_RIGHT_3_SEQUENCE=CCTCGAACATGGTTTTGATGTA
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=315,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.861
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.2791
PRIMER_LEFT_4_PENALTY=0.059210
PRIMER_RIGHT_4_PENALTY=0.219884
PRIMER_LEFT_4_SEQUENCE=ATGCCACAACNCTAAAAAGAGG
PRIMER_RIGHT_4_SEQUENCE=TGAAGTTGCAGAAACTGACCAT
PRIMER_LEFT_4=59,22
PRIMER_RIGHT_4=349,22
PRIMER_LEFT_4_TM=59.941
PRIMER_RIGHT_4_TM=59.780
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_03367 Ps_contig_mira-and-tgicl-ass_franssen_29765
SEQUENCE=CTTATTATCAACGTGTATCAAACTTTTAGATATCAGAAGTTACAAGTGTATTTGTTGCAATTTTTGTCAATTTTGGGAGTGAACATAAACTGCCATCACAAGAATGGTAGGGACTATTTTGAACAAGGCATAACAAATATTTTCCTTTACAAGTGAAAGAAGGGTACGCAATTGCATAATATCATTATATTACATGAAGAACAATCACGACAATGACAGAAGGAATTATGACAAATGCCTGAAAAACCTTATGAAGCCTTCTTTGACTTGTATCTTTTGGCAGCAACTATGTAGATAAACATATTTAAGAAGCTGAGTCCAGCTAAAAGCCAGAAAAAGTAATCGAGATGACCTTTGTTCAAGTTATCTGTAATCCATCCAGGATTTCCCCCTCGTGTAGTGAAGTAAACTACCACAGTGAGAATGAATGAACTCAAGTAATTCCCGAGCGAATTTGTCAGAAGTGACAGAGCACTGCATAAACTTCGCATAGCATCTGGAGATTGGTCATAGAAGAACTCAAGCTGCCCCACAAATGTGAATACTTCTGCTGCTCCCAATAAGAAATACTGAGGGATTTGCAAAAGTATAGTAAGGGGTACGGGGACAGCTTTATCAACAAGGTCAAGTTCTTTTGCAAGCTGCAGACGCTTAATCTCGACAATAGCAGCAGCTGACATGCACAGGACTGAAATAAAAAGACCAATTCCCATTCTTTGCAACTCTGAAAATCCCCTTTCTTTTCCAGTAAATTTCCTTGCTATGGGAATTATAAAACTGTCATAGACAGGGACCCAGAAAATAACACTAATTACATCAAAAGTTGAGAGGGAAGCTGGTGGTATTTTGAAAGAACCAATGCTAGTGTCCATCATAGTTCCTTGTTCCACAAACAATGTTGACATCTGGGCATAAACTGCAGAAAAAACTATGCCAGTAGCCCAAACTGGAAACATGCGGATCAAGATTTTTAATTCCTCCACCTGTGTGACAGTGCAAAGTCTCCACAGGTTAGAATAGTCACCACTTTTCCTCTCATCATCAGAGACTACAGCTGCTCTGTCAAGACACCTTAGTTCATCGCTATGCTGTAGTTTCCGGCTTCCTTCGATTGCAGAGCTCTTGTCTGGTGTCTCATACAGGAGACTACTATCCTCAGGGACAGCAAGATTCCGTTTCCGAAAAGATGCAGCCACAACCTGGCACATTCTTGTAAGAGGACTTCCCCCTGGTTTTTGAAACCTATATAGGGGTGTGCCTAAAAAGAAGCTTCCAATAGCTAATCCCATAAATAAAGCAGGAATGCCAAATCCAAGACCCCAGCCTGTATTATCTTGAATCCACACAATAAAACTGCTTGATATAAGGGCTCCTATGTTGATAGAAAAGTAAAACCAGTTGAAAAATGATCCCTTCTTAACCCTTTCTCGTGAATCAGTATCATCAAACTGATCTGCCCCAAAAGAGGACACGCATGGTTTAATACCACCAGTCCCGAGCGCAATCAGATAGAGACCAATGAAGAACACAGCATATTGTGCAGGAGTAGCAGGAGGGCATACAGCACCCAGACATTCAGCAGGCTTCAATGTTGGAATAGATGCAGAAAGTGTCAATGTGCACATTCCAATGAAATAAATCATGGAGAAAATGGCAATAGTCCAGTATCGTCCCCAGTAAGAATCTGCTAAAACTGCTCCAATGAGAGGTGTAAGGTAACAAGTGCCTTGCCAAGTGGTAACGTTTCTTGCCGCAGAGACATTTCCTTCATGTAGCTTGCGAGTAAGATAGGTAACAAGATTTGTTGCAATGCCATAGTATGCCAAACGTTCACAGCACTCATTACCTAGAATAAATGGGCAAGCTTTCCAATTGCCAGTATTCTTCTTAAGCACAGGCCTCCCTTTAAAGTCGACTGAGCCATCTCCTGTGTATAGCTTACTCTCTTCATCCTGAATAAGAGCTTCTTCCAAACGCGAAGAATCGTCCTCTACGGAACCCATAGCTCTGATATCTCGTTACTGACTTCAAAATCCTCTTTCGGTTTCTGCTTCTATCTTCNGGGAGGTTGCAATTTATTTATTTATGGATAATAAAAATAAATAAGATTGAATAATAAAATAAATTAATCGTTGGAATGCTCAACACAAGTTCGCCGCAATTACTTTTCGTCTTTTTGTTTGGTGTTGATTGCTTGAGAAGAAGCAACCAACAACAGCATTGCGTTGCGTCCC
TARGET=2074,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0179
PRIMER_LEFT_PENALTY=0.014720
PRIMER_RIGHT_PENALTY=0.003166
PRIMER_LEFT_SEQUENCE=CCTCTTTCGGTTTCTGCTTCTA
PRIMER_RIGHT_SEQUENCE=GTGTTGAGCATTCCAACGATTA
PRIMER_LEFT=2034,22
PRIMER_RIGHT=2147,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=60.003
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=0.1053
PRIMER_LEFT_1_PENALTY=0.102139
PRIMER_RIGHT_1_PENALTY=0.003166
PRIMER_LEFT_1_SEQUENCE=CAAAATCCTCTTTCGGTTTCTG
PRIMER_RIGHT_1_SEQUENCE=GTGTTGAGCATTCCAACGATTA
PRIMER_LEFT_1=2028,22
PRIMER_RIGHT_1=2147,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=0.1687
PRIMER_LEFT_2_PENALTY=0.014720
PRIMER_RIGHT_2_PENALTY=0.154023
PRIMER_LEFT_2_SEQUENCE=CCTCTTTCGGTTTCTGCTTCTA
PRIMER_RIGHT_2_SEQUENCE=TCTCAAGCAATCAACACCAAAC
PRIMER_LEFT_2=2034,22
PRIMER_RIGHT_2=2199,22
PRIMER_LEFT_2_TM=60.015
PRIMER_RIGHT_2_TM=60.154
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=0.2198
PRIMER_LEFT_3_PENALTY=0.216588
PRIMER_RIGHT_3_PENALTY=0.003166
PRIMER_LEFT_3_SEQUENCE=CCTTTAAAGTCGACTGAGCCAT
PRIMER_RIGHT_3_SEQUENCE=GTGTTGAGCATTCCAACGATTA
PRIMER_LEFT_3=1902,22
PRIMER_RIGHT_3=2147,22
PRIMER_LEFT_3_TM=59.783
PRIMER_RIGHT_3_TM=60.003
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=0.2562
PRIMER_LEFT_4_PENALTY=0.102139
PRIMER_RIGHT_4_PENALTY=0.154023
PRIMER_LEFT_4_SEQUENCE=CAAAATCCTCTTTCGGTTTCTG
PRIMER_RIGHT_4_SEQUENCE=TCTCAAGCAATCAACACCAAAC
PRIMER_LEFT_4=2028,22
PRIMER_RIGHT_4=2199,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=60.154
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_03368 Ps_contig_mira-and-tgicl-ass_franssen_29771
SEQUENCE=AAAGACAAGTAGGTAATACAAACGCAGAGTACGCGGGGAANATTAGGAATAGTTAACATCTCTTTATAATCAAATCAAAGTGAGGACAAACTGGGAGAACACACAAACAAGAAAACCAACCTATCTTCACTCTTCACAACATAACAGAACCACAAATGCTTGTAAGAGAAAGCCTTAGAAAAACCAAATTCTTACTTCAAAAAACCCTCAAAAACTTCAAATGTATCATCTTCGGAGGGTCCCAAAAACTCCCCAGATCTCTTTCCTTCAAACCATTCATAGGTCGAACTCGAAGCAACAACAATAGCGCAAGAACAACCTACACAAGTGATCAATTCTACAACGAGTTTTACGATATTCTTCAATCCGATCTCAATAGAATGAATCGAAACGGCGAGACTAAACCAACGGAAGAAGAAGACGCTGCTGCAACTGCAAGTAATGTAAAGCATGTGAGCTTTGTTAAACAAACTCCACAAAAGAACAGTATTGAAAACATCGCGACGGAGAAGAAGAAGAACAAAGGGAAGAACAAGAATGAAGGAGTTCATGAATTGGCATTGAAGATGAAGGAATTGGAAATGAATGATTCAGGTGATGTTGAACAAGTTCTTGATATAGAAGAAGCACTTCACTATTATTCTCGTCTTAAGAGTCCGGTTTATTTAGATATTGTGGACAAGTTTTTCACTGACATGCATTCTGAGTTTTCTGTTTCACAATCTTCTGTCGGAATCAAACATTCTAAGACAAAAGGAAGATTTGGAGCAATAAGGTTGTAGATAATATAAATCTTAATTCTTGTTTGTTTGGTGTATATGAAATGTGTTTTGTTTTTAACTTTTTTTTTTTTTTTTTTTTTTTTGTGTTTGTGTNTTNTTGTTAGGGTTTAGGGTTTACGGTTTTAGTTTTAGTTTTTTTTAC
TARGET=508,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5165
PRIMER_LEFT_PENALTY=0.189072
PRIMER_RIGHT_PENALTY=0.327468
PRIMER_LEFT_SEQUENCE=AACAATAGCGCAAGAACAACCT
PRIMER_RIGHT_SEQUENCE=ATGCCAATTCATGAACTCCTTC
PRIMER_LEFT=299,22
PRIMER_RIGHT=560,22
PRIMER_LEFT_TM=60.189
PRIMER_RIGHT_TM=60.327
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=0.5752
PRIMER_LEFT_1_PENALTY=0.247716
PRIMER_RIGHT_1_PENALTY=0.327468
PRIMER_LEFT_1_SEQUENCE=ATTCATAGGTCGAACTCGAAGC
PRIMER_RIGHT_1_SEQUENCE=ATGCCAATTCATGAACTCCTTC
PRIMER_LEFT_1=274,22
PRIMER_RIGHT_1=560,22
PRIMER_LEFT_1_TM=59.752
PRIMER_RIGHT_1_TM=60.327
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.6252
PRIMER_LEFT_2_PENALTY=0.297699
PRIMER_RIGHT_2_PENALTY=0.327468
PRIMER_LEFT_2_SEQUENCE=CGAACTCGAAGCAACAACAATA
PRIMER_RIGHT_2_SEQUENCE=ATGCCAATTCATGAACTCCTTC
PRIMER_LEFT_2=284,22
PRIMER_RIGHT_2=560,22
PRIMER_LEFT_2_TM=60.298
PRIMER_RIGHT_2_TM=60.327
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.7497
PRIMER_LEFT_3_PENALTY=0.422232
PRIMER_RIGHT_3_PENALTY=0.327468
PRIMER_LEFT_3_SEQUENCE=TGCTGCAACTGCAAGTAATGTA
PRIMER_RIGHT_3_SEQUENCE=ATGCCAATTCATGAACTCCTTC
PRIMER_LEFT_3=424,22
PRIMER_RIGHT_3=560,22
PRIMER_LEFT_3_TM=59.578
PRIMER_RIGHT_3_TM=60.327
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=137
PRIMER_PAIR_PENALTY_4=0.8296
PRIMER_LEFT_4_PENALTY=0.502108
PRIMER_RIGHT_4_PENALTY=0.327468
PRIMER_LEFT_4_SEQUENCE=CTGCTGCAACTGCAAGTAATGT
PRIMER_RIGHT_4_SEQUENCE=ATGCCAATTCATGAACTCCTTC
PRIMER_LEFT_4=423,22
PRIMER_RIGHT_4=560,22
PRIMER_LEFT_4_TM=60.502
PRIMER_RIGHT_4_TM=60.327
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_03369 Ps_contig_mira-and-tgicl-ass_franssen_29772
SEQUENCE=ACCTAAGAGTGCAAAGCAAACAAGAGCAGAGTTAGAAGCTACTTCGAATTAGGTAAAATATAATTTGACATGGGAAATAATCCCAACCGGTCCTGGTCTCTTCAACGACTACACAGATTATCCATGTCGAGTACAACTAGGTAGATATACATTCATCGATGTGACATATACAGAGATCAAACGTAATTGCAGTGATAGTGATGATGAAGTTCCCAGTGATAATGATGAAGTTCCCGGTGATAGTGAGGATGAAGTTCCCAGTGATAGTGACGATGAAGTTCCCAGTGATAGTGACGATGAAGTTCCCAGTGATGATGATGAAGTTCCCAGTGATGATGATGATGAAGTTCCCAGTGATAGTGATGATGAAGTTACCAAAGTGACCACGATGTTATACACCGTAGATAGGTCGTCAACCTGTGAACTCAAATTTGAGAGACGTCATAAAGAGGCATCATTATCTCTCCATGATGCAGACAATTGTTCCAATTTCACTGGTGTTGATTTGGAAATTATAATAAGAAAAACTGATAAGGGGTATCTTGAGATACCTGTTCCCGAAATCGCCGCTATTGCAAATAGCAGAGAGGTATGCATTTTAAATTGGGGTGTTAATTCTAACAGAACCATCAATGCATGGGGAGAAGTAAAAGAATATACAAACATTCTCTATGGTAAAGGAAGGAGGGGAAGTCTTGTTGTTATAGAGTCTAAGAAAAAGAGTATAAGTGATCCAAATCCCTATATGGTCACTGTATTACACTATTTAGCTGTTGATGACGGAAATGTTTTCAATCCAAAATTGGATATTGGCTTTTCTGTGGTTGTAAAGATTCAAACATCAGATGACTTGGGATTGAAATATACTGTAGAGGGTCCAGAGAGACACCCTAGTTCATCATTATTTTACATGTTTGAACGAGTAAAAAGACTTAATGGGGCATGGAAGTCTAATTTGTGTCCTCATTGTGCAAATCTTCAGATGCGGCGTAAATCCTGGCAGACCTCTGAAAGTGAAGACAGCGATTATGTTCCACGACGACATGGTAATGGTAAGCAAAAGTCAGAAAGTATGGTTGCCAATGATTCTAACGTTAAGGGAAATTTTAATGGTAGTTTGTTTGGGAAGTATTTCCATTTTCATAAAAGGTGAAATATGTAATGGAATCCTTTAGTATGAAAAGAAAGAAAATAATATTAATAGTAAAATAAAGTGGTTAGGGCAAGGTTAGCCACGGATTAAGATGGGTACCATACCTAACTCATGTGTAGTCAATAATAATAATAAGCCATATTTGTAACTAAGTGAGTTGTTAATTAGAGAGCATTGGTATGTAAATTTGTCTGTTAACCATCTTAATGTATGGGACACCTGGCTC
TARGET=310,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0418
PRIMER_LEFT_PENALTY=0.026532
PRIMER_RIGHT_PENALTY=0.015253
PRIMER_LEFT_SEQUENCE=GTGATGATGAAGTTCCCAGTGA
PRIMER_RIGHT_SEQUENCE=TGCCTCTTTATGACGTCTCTCA
PRIMER_LEFT=197,22
PRIMER_RIGHT=453,22
PRIMER_LEFT_TM=59.973
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.2022
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=0.175676
PRIMER_LEFT_1_SEQUENCE=GTGATGATGAAGTTCCCAGTGA
PRIMER_RIGHT_1_SEQUENCE=ACGGTGTATAACATCGTGGTCA
PRIMER_LEFT_1=197,22
PRIMER_RIGHT_1=401,22
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=60.176
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.2197
PRIMER_LEFT_2_PENALTY=0.026532
PRIMER_RIGHT_2_PENALTY=0.193120
PRIMER_LEFT_2_SEQUENCE=GTGATGATGAAGTTCCCAGTGA
PRIMER_RIGHT_2_SEQUENCE=TTTGAGTTCACAGGTTGACGAC
PRIMER_LEFT_2=197,22
PRIMER_RIGHT_2=429,22
PRIMER_LEFT_2_TM=59.973
PRIMER_RIGHT_2_TM=60.193
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.3324
PRIMER_LEFT_3_PENALTY=0.207756
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=ATGATGAAGTTCCCGGTGATAG
PRIMER_RIGHT_3_SEQUENCE=TCAACACCAGTGAAATTGGAAC
PRIMER_LEFT_3=221,22
PRIMER_RIGHT_3=503,22
PRIMER_LEFT_3_TM=60.208
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.3834
PRIMER_LEFT_4_PENALTY=0.207756
PRIMER_RIGHT_4_PENALTY=0.175676
PRIMER_LEFT_4_SEQUENCE=ATGATGAAGTTCCCGGTGATAG
PRIMER_RIGHT_4_SEQUENCE=ACGGTGTATAACATCGTGGTCA
PRIMER_LEFT_4=221,22
PRIMER_RIGHT_4=401,22
PRIMER_LEFT_4_TM=60.208
PRIMER_RIGHT_4_TM=60.176
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_03370 Ps_contig_mira-and-tgicl-ass_franssen_29772
SEQUENCE=ACCTAAGAGTGCAAAGCAAACAAGAGCAGAGTTAGAAGCTACTTCGAATTAGGTAAAATATAATTTGACATGGGAAATAATCCCAACCGGTCCTGGTCTCTTCAACGACTACACAGATTATCCATGTCGAGTACAACTAGGTAGATATACATTCATCGATGTGACATATACAGAGATCAAACGTAATTGCAGTGATAGTGATGATGAAGTTCCCAGTGATAATGATGAAGTTCCCGGTGATAGTGAGGATGAAGTTCCCAGTGATAGTGACGATGAAGTTCCCAGTGATAGTGACGATGAAGTTCCCAGTGATGATGATGAAGTTCCCAGTGATGATGATGATGAAGTTCCCAGTGATAGTGATGATGAAGTTACCAAAGTGACCACGATGTTATACACCGTAGATAGGTCGTCAACCTGTGAACTCAAATTTGAGAGACGTCATAAAGAGGCATCATTATCTCTCCATGATGCAGACAATTGTTCCAATTTCACTGGTGTTGATTTGGAAATTATAATAAGAAAAACTGATAAGGGGTATCTTGAGATACCTGTTCCCGAAATCGCCGCTATTGCAAATAGCAGAGAGGTATGCATTTTAAATTGGGGTGTTAATTCTAACAGAACCATCAATGCATGGGGAGAAGTAAAAGAATATACAAACATTCTCTATGGTAAAGGAAGGAGGGGAAGTCTTGTTGTTATAGAGTCTAAGAAAAAGAGTATAAGTGATCCAAATCCCTATATGGTCACTGTATTACACTATTTAGCTGTTGATGACGGAAATGTTTTCAATCCAAAATTGGATATTGGCTTTTCTGTGGTTGTAAAGATTCAAACATCAGATGACTTGGGATTGAAATATACTGTAGAGGGTCCAGAGAGACACCCTAGTTCATCATTATTTTACATGTTTGAACGAGTAAAAAGACTTAATGGGGCATGGAAGTCTAATTTGTGTCCTCATTGTGCAAATCTTCAGATGCGGCGTAAATCCTGGCAGACCTCTGAAAGTGAAGACAGCGATTATGTTCCACGACGACATGGTAATGGTAAGCAAAAGTCAGAAAGTATGGTTGCCAATGATTCTAACGTTAAGGGAAATTTTAATGGTAGTTTGTTTGGGAAGTATTTCCATTTTCATAAAAGGTGAAATATGTAATGGAATCCTTTAGTATGAAAAGAAAGAAAATAATATTAATAGTAAAATAAAGTGGTTAGGGCAAGGTTAGCCACGGATTAAGATGGGTACCATACCTAACTCATGTGTAGTCAATAATAATAATAAGCCATATTTGTAACTAAGTGAGTTGTTAATTAGAGAGCATTGGTATGTAAATTTGTCTGTTAACCATCTTAATGTATGGGACACCTGGCTC
TARGET=331,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0418
PRIMER_LEFT_PENALTY=0.026532
PRIMER_RIGHT_PENALTY=0.015253
PRIMER_LEFT_SEQUENCE=GTGATGATGAAGTTCCCAGTGA
PRIMER_RIGHT_SEQUENCE=TGCCTCTTTATGACGTCTCTCA
PRIMER_LEFT=197,22
PRIMER_RIGHT=453,22
PRIMER_LEFT_TM=59.973
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.2022
PRIMER_LEFT_1_PENALTY=0.026532
PRIMER_RIGHT_1_PENALTY=0.175676
PRIMER_LEFT_1_SEQUENCE=GTGATGATGAAGTTCCCAGTGA
PRIMER_RIGHT_1_SEQUENCE=ACGGTGTATAACATCGTGGTCA
PRIMER_LEFT_1=197,22
PRIMER_RIGHT_1=401,22
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=60.176
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.2197
PRIMER_LEFT_2_PENALTY=0.026532
PRIMER_RIGHT_2_PENALTY=0.193120
PRIMER_LEFT_2_SEQUENCE=GTGATGATGAAGTTCCCAGTGA
PRIMER_RIGHT_2_SEQUENCE=TTTGAGTTCACAGGTTGACGAC
PRIMER_LEFT_2=197,22
PRIMER_RIGHT_2=429,22
PRIMER_LEFT_2_TM=59.973
PRIMER_RIGHT_2_TM=60.193
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.3324
PRIMER_LEFT_3_PENALTY=0.207756
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=ATGATGAAGTTCCCGGTGATAG
PRIMER_RIGHT_3_SEQUENCE=TCAACACCAGTGAAATTGGAAC
PRIMER_LEFT_3=221,22
PRIMER_RIGHT_3=503,22
PRIMER_LEFT_3_TM=60.208
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.3834
PRIMER_LEFT_4_PENALTY=0.207756
PRIMER_RIGHT_4_PENALTY=0.175676
PRIMER_LEFT_4_SEQUENCE=ATGATGAAGTTCCCGGTGATAG
PRIMER_RIGHT_4_SEQUENCE=ACGGTGTATAACATCGTGGTCA
PRIMER_LEFT_4=221,22
PRIMER_RIGHT_4=401,22
PRIMER_LEFT_4_TM=60.208
PRIMER_RIGHT_4_TM=60.176
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_03371 Ps_contig_mira-and-tgicl-ass_franssen_29772
SEQUENCE=ACCTAAGAGTGCAAAGCAAACAAGAGCAGAGTTAGAAGCTACTTCGAATTAGGTAAAATATAATTTGACATGGGAAATAATCCCAACCGGTCCTGGTCTCTTCAACGACTACACAGATTATCCATGTCGAGTACAACTAGGTAGATATACATTCATCGATGTGACATATACAGAGATCAAACGTAATTGCAGTGATAGTGATGATGAAGTTCCCAGTGATAATGATGAAGTTCCCGGTGATAGTGAGGATGAAGTTCCCAGTGATAGTGACGATGAAGTTCCCAGTGATAGTGACGATGAAGTTCCCAGTGATGATGATGAAGTTCCCAGTGATGATGATGATGAAGTTCCCAGTGATAGTGATGATGAAGTTACCAAAGTGACCACGATGTTATACACCGTAGATAGGTCGTCAACCTGTGAACTCAAATTTGAGAGACGTCATAAAGAGGCATCATTATCTCTCCATGATGCAGACAATTGTTCCAATTTCACTGGTGTTGATTTGGAAATTATAATAAGAAAAACTGATAAGGGGTATCTTGAGATACCTGTTCCCGAAATCGCCGCTATTGCAAATAGCAGAGAGGTATGCATTTTAAATTGGGGTGTTAATTCTAACAGAACCATCAATGCATGGGGAGAAGTAAAAGAATATACAAACATTCTCTATGGTAAAGGAAGGAGGGGAAGTCTTGTTGTTATAGAGTCTAAGAAAAAGAGTATAAGTGATCCAAATCCCTATATGGTCACTGTATTACACTATTTAGCTGTTGATGACGGAAATGTTTTCAATCCAAAATTGGATATTGGCTTTTCTGTGGTTGTAAAGATTCAAACATCAGATGACTTGGGATTGAAATATACTGTAGAGGGTCCAGAGAGACACCCTAGTTCATCATTATTTTACATGTTTGAACGAGTAAAAAGACTTAATGGGGCATGGAAGTCTAATTTGTGTCCTCATTGTGCAAATCTTCAGATGCGGCGTAAATCCTGGCAGACCTCTGAAAGTGAAGACAGCGATTATGTTCCACGACGACATGGTAATGGTAAGCAAAAGTCAGAAAGTATGGTTGCCAATGATTCTAACGTTAAGGGAAATTTTAATGGTAGTTTGTTTGGGAAGTATTTCCATTTTCATAAAAGGTGAAATATGTAATGGAATCCTTTAGTATGAAAAGAAAGAAAATAATATTAATAGTAAAATAAAGTGGTTAGGGCAAGGTTAGCCACGGATTAAGATGGGTACCATACCTAACTCATGTGTAGTCAATAATAATAATAAGCCATATTTGTAACTAAGTGAGTTGTTAATTAGAGAGCATTGGTATGTAAATTTGTCTGTTAACCATCTTAATGTATGGGACACCTGGCTC
TARGET=1275,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0892
PRIMER_LEFT_PENALTY=0.477034
PRIMER_RIGHT_PENALTY=0.612170
PRIMER_LEFT_SEQUENCE=AGGTTAGCCACGGATTAAGATG
PRIMER_RIGHT_SEQUENCE=GCCAGGTGTCCCATACATTAAG
PRIMER_LEFT=1225,22
PRIMER_RIGHT=1376,22
PRIMER_LEFT_TM=59.523
PRIMER_RIGHT_TM=60.612
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=1.0935
PRIMER_LEFT_1_PENALTY=0.481326
PRIMER_RIGHT_1_PENALTY=0.612170
PRIMER_LEFT_1_SEQUENCE=CAAGGTTAGCCACGGATTAAGA
PRIMER_RIGHT_1_SEQUENCE=GCCAGGTGTCCCATACATTAAG
PRIMER_LEFT_1=1223,22
PRIMER_RIGHT_1=1376,22
PRIMER_LEFT_1_TM=60.481
PRIMER_RIGHT_1_TM=60.612
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=154
PRIMER_PAIR_PENALTY_2=1.4726
PRIMER_LEFT_2_PENALTY=0.860451
PRIMER_RIGHT_2_PENALTY=0.612170
PRIMER_LEFT_2_SEQUENCE=AAGTGGTTAGGGCAAGGTTAGC
PRIMER_RIGHT_2_SEQUENCE=GCCAGGTGTCCCATACATTAAG
PRIMER_LEFT_2=1211,22
PRIMER_RIGHT_2=1376,22
PRIMER_LEFT_2_TM=60.860
PRIMER_RIGHT_2_TM=60.612
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=1.5944
PRIMER_LEFT_3_PENALTY=0.477034
PRIMER_RIGHT_3_PENALTY=1.117398
PRIMER_LEFT_3_SEQUENCE=AGGTTAGCCACGGATTAAGATG
PRIMER_RIGHT_3_SEQUENCE=GAGCCAGGTGTCCCATACATTA
PRIMER_LEFT_3=1225,22
PRIMER_RIGHT_3=1378,22
PRIMER_LEFT_3_TM=59.523
PRIMER_RIGHT_3_TM=61.117
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=1.5987
PRIMER_LEFT_4_PENALTY=0.481326
PRIMER_RIGHT_4_PENALTY=1.117398
PRIMER_LEFT_4_SEQUENCE=CAAGGTTAGCCACGGATTAAGA
PRIMER_RIGHT_4_SEQUENCE=GAGCCAGGTGTCCCATACATTA
PRIMER_LEFT_4=1223,22
PRIMER_RIGHT_4=1378,22
PRIMER_LEFT_4_TM=60.481
PRIMER_RIGHT_4_TM=61.117
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=156
=
PRIMER_SEQUENCE_ID=SSR_03372 Ps_contig_mira-and-tgicl-ass_franssen_29773
SEQUENCE=AGCGGGGACCTAAGAGTGCAAAGCAAACAAGAGCAGAGTTAGAAGCTACTTCGAATTAGGTAAAATATAATTTGACATGGGAAATAGTCTCAACGGTGCTAGTCTCTTCAACGACGTCACAAATTATCCATGTCGGATACAACTAGGTAGATATGCATTCATCGATGTGACATATACAGAGAACAAACATGATTGCAGTGATAGTGATGATGAAGTTCCCAGTGATAATGATGAAGTTCCCGGTGATAGTGATGATGAATATCCCAGTGATGATGATGATGATGATGAATTTCCCTATGATGATGAATTTCCCAGTGATGATGATGAAGTTGATAATGATGATGAATTTCCTAGGGATGAAGATGAAGTTATCAAAGTGACCAAGATGTTATACACGGTAGATAGGTCGTCCATATGTGAACTCAAATTTGAGAGAAGTCATAAAGAGGCATCATTATCTCTCCATGATGTAGACAATGGTTCCAATTCCAACGGTGTTGATGTTAAAATTATAATAAGAAATACTGATGAGGGGTATCTTGAGATACCTTTTCCTGTATTCTTCGCTAGAGGCGGAGGGATGTACATTTTCAATTGGGATAATAATTCTAATAGAACCATCAATGCATGGGGAGGTGTGACAGAATTAACAACCTTTCTCTTTGGTAAAGGAAAAAGGGGAAGTCTTGTTGTTATAGAGTCTAAGAAAAAGAGTATAAGTGATCCAAATCCCTATATGGTCACTGTGGTTCACTATTTAGCTATTGATGACGGAAATTTTTTCAATGCAAAAATGGATATTGGCCTTTCTGTGATGGTAAAGATTCAACCATCAGATGATTTGGGATTGAAATTTACTGTAGAGGGCCCAGAGAAACACCCTAGTTCATCATTACTTTACATGTTTGAACGAGTAAAAAGACTTAATGGGGCATGGAAGCCTAATTTGTGTCCTCATTGTGCAAATCTTCGGCTTAAATCCTTGCAGATGTTTGAAAGTGAAGACAGTGATTATTTTCCACGACGACATGGTAATGGTTATAAGCAGAAGTCAGAAAGTATGGTTGCCAGTGATTCTCACGTTAAGGGAAATTATAATAGTAGTTTGTTGGGGAAGTATTTCCATTTTTATAAAAAGTAAAATAGGTAATGGAATCCTTTAATAAGAAGAGAAAGAAAATAAAAAGGTTAGGGCAAGGTTAGCCAGGCTAAAGATGGATACCATACCTAACTAAGTAGGTTTTTAATTAGAGAACATTTTAAAATATTGGTAAGTAATTTTGTGTGCAGCACACACCCTTATCTTATAACTCTCAAACAAATGGATAGGAAAGATGCAATCGCATGCTTTAGTTCCTGCAGAATCATTGTACACTGCAGAATCATTTTCATGTCTTCTAATAACTACTATCTGCACTTTTATGAATTATAATATGAACCAAGCAAATTAGCTTCAYTATATTGAATGATCAAGTACAAAGTTACTTGACAATTGAC
TARGET=268,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03373 Ps_contig_mira-and-tgicl-ass_franssen_29773
SEQUENCE=AGCGGGGACCTAAGAGTGCAAAGCAAACAAGAGCAGAGTTAGAAGCTACTTCGAATTAGGTAAAATATAATTTGACATGGGAAATAGTCTCAACGGTGCTAGTCTCTTCAACGACGTCACAAATTATCCATGTCGGATACAACTAGGTAGATATGCATTCATCGATGTGACATATACAGAGAACAAACATGATTGCAGTGATAGTGATGATGAAGTTCCCAGTGATAATGATGAAGTTCCCGGTGATAGTGATGATGAATATCCCAGTGATGATGATGATGATGATGAATTTCCCTATGATGATGAATTTCCCAGTGATGATGATGAAGTTGATAATGATGATGAATTTCCTAGGGATGAAGATGAAGTTATCAAAGTGACCAAGATGTTATACACGGTAGATAGGTCGTCCATATGTGAACTCAAATTTGAGAGAAGTCATAAAGAGGCATCATTATCTCTCCATGATGTAGACAATGGTTCCAATTCCAACGGTGTTGATGTTAAAATTATAATAAGAAATACTGATGAGGGGTATCTTGAGATACCTTTTCCTGTATTCTTCGCTAGAGGCGGAGGGATGTACATTTTCAATTGGGATAATAATTCTAATAGAACCATCAATGCATGGGGAGGTGTGACAGAATTAACAACCTTTCTCTTTGGTAAAGGAAAAAGGGGAAGTCTTGTTGTTATAGAGTCTAAGAAAAAGAGTATAAGTGATCCAAATCCCTATATGGTCACTGTGGTTCACTATTTAGCTATTGATGACGGAAATTTTTTCAATGCAAAAATGGATATTGGCCTTTCTGTGATGGTAAAGATTCAACCATCAGATGATTTGGGATTGAAATTTACTGTAGAGGGCCCAGAGAAACACCCTAGTTCATCATTACTTTACATGTTTGAACGAGTAAAAAGACTTAATGGGGCATGGAAGCCTAATTTGTGTCCTCATTGTGCAAATCTTCGGCTTAAATCCTTGCAGATGTTTGAAAGTGAAGACAGTGATTATTTTCCACGACGACATGGTAATGGTTATAAGCAGAAGTCAGAAAGTATGGTTGCCAGTGATTCTCACGTTAAGGGAAATTATAATAGTAGTTTGTTGGGGAAGTATTTCCATTTTTATAAAAAGTAAAATAGGTAATGGAATCCTTTAATAAGAAGAGAAAGAAAATAAAAAGGTTAGGGCAAGGTTAGCCAGGCTAAAGATGGATACCATACCTAACTAAGTAGGTTTTTAATTAGAGAACATTTTAAAATATTGGTAAGTAATTTTGTGTGCAGCACACACCCTTATCTTATAACTCTCAAACAAATGGATAGGAAAGATGCAATCGCATGCTTTAGTTCCTGCAGAATCATTGTACACTGCAGAATCATTTTCATGTCTTCTAATAACTACTATCTGCACTTTTATGAATTATAATATGAACCAAGCAAATTAGCTTCAYTATATTGAATGATCAAGTACAAAGTTACTTGACAATTGAC
TARGET=316,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03374 Ps_contig_mira-and-tgicl-ass_franssen_29783
SEQUENCE=TTCTAAATACGTAATTTTTAAACTAAATAGAACAATAGTTAGCATCATCATCATCAATCTCTCTTCAACCAAACGTGCTTCTTCTTCCCACCACTTCAATTCCATCGATTCGACCCATCAAAACCAACTACATCAAGATGAAAACAAACCGTTTTACTGCTTCTGCTGTTGCTGTTGCTGAAGATTCCGTTTCTTCACAGTCCTCTCCTTCTCTCCAGACACCGCCTCAGTCTCAGAAACTAGGAGTAGTTGTGAAACCAACAGATAAACCAAGACTCGTGTTGAAGTTCGTTTGGATGGAGAAGAACATTGGTATCGCACTCGACCAAATGGTACCTGGCTACGGAACCATTCCTCTAAGCCCGTACTACTTTTGGCCAAGGAAAGACGCTTGGGAAGAGCTCAAGGAGTTGCTAGAGAGTAAGCCATGGATATCTCAAAAGCAGATGATTATTCTTCTCAATCAAGCCACTGATATTATCAATTTGTGGCAACAAAGTGGTGGTAATTTGTCCTCCTAAGGCTCAAATCTAGGGTATTCCAAACAATGTTTTTTGTTTTTGTATCAATAGAATGTTTCACTTTTTAATGTTATGGCTTATCTGTTGTATTCTATTTGCTCTTATCATGTACATCAGACAAGTACCAAATTCTTTTAGATGAACAATTACTTTTGGGTCTATCGATGATTGTAGTTTTATTTTATCAATGAAATTTCTGCATAGACAAAAATTTGTATTTGCTGATTGTTAATTTGTGTCTATGT
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03375 Ps_contig_mira-and-tgicl-ass_franssen_29785
SEQUENCE=CGTTTGAGGCAATGCGTAGAATGTTCCTGGCTGGACTCTTGATGGAACTAAATAATCTCGGGCACCTTCTGGTGTGGATCTCGACAGTATTGGAGTTTCAATCTCCACAAAACCATGTATGTCTTCTAGATGTCTTCGTATGAGTTTAACCACTTTATGCCGCAGCAATATGTTAAAATTCATTTGCTGCCTTCTTAGATCAAGACATCGATACCTCAAACGGATTTCCTCTTTAAGAGAATCTTTTGCATCATCTGATGTAGTAACTAAGAATGGTAACTTTGAATTCACAGAATTCAACACGTGAACCTCATTTGCAGCAACCTCGATGAAGCCTGTTTTCATCTTCTTGTTGATTGATTCATTCGGACGTGACCTGACGACACCTTCAATGGAAACGACATACTCCAATCTGAGATCATTGATAACAGAATGTGCAAGTGGAAAATCATCCGGAAGTGTTGTAACCTGAACAATCCCGGTATGGTCTCTAAGATTAAGAAAAGTGAGTCCACCGTGGACTCTATGAAGTGCAACCCAACCACAAAGCTGAACCTTTTTACCAACATCATTCAAAGACAATTCGCCACAAAAAATGAGTCCTTGTAACCCATTGAAGCGATTCATTGAAGGGTAAAAGCGGTGGTGGTGGTTTAGCATTGGAAGTGAGTGTTTCAGAGGCAGAAGGAAGTGGTTGAATCGAAGCTGAAAGAGAAGAAACGGTTCTNGGTAGTTGAAACTAACGTTGGAATTGTAGTGGAAACCCTAGAANGAAAGAAAAAGGATGGTCTAGGTCGAAGAACCATCAATGGCATTG
TARGET=642,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2590
PRIMER_LEFT_PENALTY=0.149516
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=CAATCCCGGTATGGTCTCTAAG
PRIMER_RIGHT_SEQUENCE=TTCGATTCAACCACTTCCTTCT
PRIMER_LEFT=473,22
PRIMER_RIGHT=703,22
PRIMER_LEFT_TM=59.850
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.2590
PRIMER_LEFT_1_PENALTY=0.149516
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=CAATCCCGGTATGGTCTCTAAG
PRIMER_RIGHT_1_SEQUENCE=CTTCGATTCAACCACTTCCTTC
PRIMER_LEFT_1=473,22
PRIMER_RIGHT_1=704,22
PRIMER_LEFT_1_TM=59.850
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.2867
PRIMER_LEFT_2_PENALTY=0.066849
PRIMER_RIGHT_2_PENALTY=0.219884
PRIMER_LEFT_2_SEQUENCE=CGGAAGTGTTGTAACCTGAACA
PRIMER_RIGHT_2_SEQUENCE=TGAAACACTCACTTCCAATGCT
PRIMER_LEFT_2=453,22
PRIMER_RIGHT_2=676,22
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=59.780
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.2867
PRIMER_LEFT_3_PENALTY=0.066849
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=CGGAAGTGTTGTAACCTGAACA
PRIMER_RIGHT_3_SEQUENCE=CTGAAACACTCACTTCCAATGC
PRIMER_LEFT_3=453,22
PRIMER_RIGHT_3=677,22
PRIMER_LEFT_3_TM=60.067
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=0.2890
PRIMER_LEFT_4_PENALTY=0.030114
PRIMER_RIGHT_4_PENALTY=0.258908
PRIMER_LEFT_4_SEQUENCE=GCGATTCATTGAAGGGTAAAAG
PRIMER_RIGHT_4_SEQUENCE=AGGGTTTCCACTACAATTCCAA
PRIMER_LEFT_4=618,22
PRIMER_RIGHT_4=766,22
PRIMER_LEFT_4_TM=59.970
PRIMER_RIGHT_4_TM=59.741
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_03376 Ps_contig_mira-and-tgicl-ass_franssen_29789
SEQUENCE=CTTTCTCTTTCGTATCGAGTTGTGTTTTTTTGTTTTGTTTTTGCCACCTTTAAGGTTTTGGTCGGAAAGTTTAGATTTTTCACACACCCTTTTAACTCTAAGCCATGATTTCCTCGTTGAGACAACCTAGTATTTCGATCAGTGGTTCCGATGTTGTTTTAAGGAAAAGACATGCAACCCTAATTCAGCTACGACCACAATCGTTTTCACCTTTCTCGTCAAGAGAGAAATCACAGAGATCTGTTGTTTCAACGAAGAAGCCTCTTCATCTTGCATGTCTTGGTGTTGGAAATTTTGGGTCTGTGAAGAATTTTGAGTCCGAGGCGAGTTTTGGGCAAAGTGATTTGGTGAAGTGTGGGGCTTATGAGGCTGATAGATCAGAGGTTGAAGGTGGTGATGGAACACCATCAGAGGCTGCTAAGAAGGTGAAAATTGGGATATATTTTGCGACTTGGTGGGCTTTGAATGTTGTGTTTAATATTTATAACAAGAAGGTTTTGAATGCGTATCCTTACCCTTGGCTTACTTCGACTCTTTCGCTGGCTTGTGGCTCTCTTATGATGTTGATCTCTTGGGCTACTAGGATTGCTGAAGCTCCTAAGACTGATCTTGAGTTTTGGAAGACTTTGTTCCCTGTTGCTGTTGCACACACGATTGGACATGTAGCGGCTACGGTTAGTATGTCCAAAGTTGCGGTATCATTTACGCATATCATTAAGAGTGGCGAGCCTGCTTTTAGTGTTCTCGTTTCGAGGTTTATTTTGGGCGAGACCTTCCCTGTGCCGGTCTATCTGTCTTTACTTCCGATTATTGGTGGATGTGCACTTGCTGCTGTGACCGAGCTCAATTTCAATATGATCGGTTTTATGGGGGCTATGATATCAAATCTGGCATTTGTGTTCCGAAATATCTTTTCCAAAAAGGGGATGAAGGGAAAATCCGTTAGCGGAATGAATTACTATGCCTGTTTATCTATTTTGTCCCTTGCAATTCTCACACCCTTTGCAATCGCCGTGGAAGGACCAGCAATGTGGGCTGCCGGATGGCAAACAGCTCTCTCTGAAATCGGACCCCAATTCATATGGTGGGTAGCAGCTCAGAGTATATTCTATCATCTATACAATCAGGTGTCTTACATGTCGTTGGATGAGATCTCTCCTTTGACGTTTAGCATTGGAAACACCATGAAACGTATATCGGTCATAGTCTCTTCAATTATCATCTTCCACACACCAATTCAGCCCGTCAATGCTCTCGGAGCCGCAATTGCCGTATTTGGAACCTTCTTATACTCACAGGCAAAACAATAGAGTTGAGGAATAAAGAAATCCTCATTTTCAAACCTACAGTTGATTTTGGAACATTTGGCTGCCGGATTTTTGTATGACTAGGATGATCTGGCTTTTTGTGCATCTGTAGATAATAAATAAATAATAAGAATCAGAATAATGCTACTGAGAAATATTATCCTTAAACAGATGATTAGTGTAGTTTCTTTGTTTTAYTTTTTTTRGTTCTNANRTNCNAANNAAGTCCGAACTAGTAATTTAACTT
TARGET=1422,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03377 Ps_contig_mira-and-tgicl-ass_franssen_29803
SEQUENCE=ATTAATTTTTTTTATTATTCATTAATAATATAAGTAAATATTCCTTTAGAGCAACATTGATTATCCAAACACAGTAATACAACTGTACCATACAGAAACACACGAATTGCAATTGTTCAATTGCCTCTCAATCGCCAAGTTTGTAAAAAATAAAGACAATGCAAATTTTGCAGCCACGGGACGGGAACACTCATTATTCTCTGCCTGCATCAGATGAGAACTTATCAGCACACGAATGGTTCATCAACATGGTGGTCACTGCAGTAGAGTCCAGGTTTTTGAGTGAAACCGGCCTTCTGTAGCTTCTGCCTAGCCTTGAGAACAAGCTCTGAATATGTAACTGAACCATCAGTCTCCTCAATTATAGCTTGTATTGCATTACTAAAAGCTCCATAAGCATTAGCTGCATTTCCAGCTGGACAAGCATCTGCTGAAGTCTGGTCTGTCTGACAGCCGCTCATCAGAATCCCACCGCTTGGCATACCGCGCTTTGATGAACCAGCATATACTTCATGCTTACTTCCCACTTCCGTCTCCATGGCAGGTTTTGCATATCCCTCATCATTCTCATCCAGCTTTTGCTTGAGAAACTGTTGGGCAAGACTACCTACCATTCCCAGTATCCCACCATGTTCACCTTCACCATCTCCTTGGAGTTTGTCCAGGATAACCTTCATAAACTTCTTCACTTTGGGGCTAGCATCTTCCCCAAAAACATCAAAAAGGGTAGGTCTCAGTTTACCAACATCTATATCATCTTTTCCAGTTTTCTGCTTGAGTATCTCAATGAGGGTTGAGAGTGGCAGAGACTTACTCCTTAGATAACCATAGTTGCCATGTGCGAGTTCGCTGTCCCCATCTTCTTCATCCCTGTGGTTATGACGACGCAATCCTGAAGGGACGTGAAATCCCCTGGACTCAATGGCATCCTCAACTTTCCTATGCAGAAAGCTTGAGAATCCAAAGCCAGATTCAGATTGTTCCTCACCTCCTTTAGTGCTCTCTCCTATCTGCTCCTTAGCTTCTTCAATTAAGCCACCACTATGGCAAGAATCAGATACAATTGTCAGCCTACATCCTCTAGGTATCCCATTCACAAATTCCTTGAAATCATCATCAGTGATGAGATTCATATCAGAAGGAACGATACATTCATCAAAACCAGTATCATCATCCTCTCCCGTTTCAGCAGGAAGACGCGTACCATGTCCACTATAATGAACGAACAACACATCTCCTTCCTCCGCCGATCGGATCAACTCCGACAGCACACGGCGTATGTTCTTTCCCGTCGGCTCCGTATACGACGAATCGGTGTCGATCAAAACGGTGATGTCGTCTTCGGAAAATCCGTATCGTTCGATCAAGCATTTGTGCATCCTCCATACGTCGTTTACGCATCCTCTTAACTCTGCTTTTGTTCCGGGATAGTTGATCCCAATCAACACAGCTTTTTTCGCCATTTGATTACAGAGTCTTTTGTTGGATCTAAAGAAGAAGATGAAGAAAAAAAAAGAAGTAGTTATTTGACTATTTTCTTGCTTTCTTTTTTAGCTATGATTGATTGATACACCAAAACTGTCATTTGTCATTCTATCATCTTGTGAATTTATTATTATTATTTTTTAT
TARGET=1558,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03378 Ps_contig_mira-and-tgicl-ass_franssen_29803
SEQUENCE=ATTAATTTTTTTTATTATTCATTAATAATATAAGTAAATATTCCTTTAGAGCAACATTGATTATCCAAACACAGTAATACAACTGTACCATACAGAAACACACGAATTGCAATTGTTCAATTGCCTCTCAATCGCCAAGTTTGTAAAAAATAAAGACAATGCAAATTTTGCAGCCACGGGACGGGAACACTCATTATTCTCTGCCTGCATCAGATGAGAACTTATCAGCACACGAATGGTTCATCAACATGGTGGTCACTGCAGTAGAGTCCAGGTTTTTGAGTGAAACCGGCCTTCTGTAGCTTCTGCCTAGCCTTGAGAACAAGCTCTGAATATGTAACTGAACCATCAGTCTCCTCAATTATAGCTTGTATTGCATTACTAAAAGCTCCATAAGCATTAGCTGCATTTCCAGCTGGACAAGCATCTGCTGAAGTCTGGTCTGTCTGACAGCCGCTCATCAGAATCCCACCGCTTGGCATACCGCGCTTTGATGAACCAGCATATACTTCATGCTTACTTCCCACTTCCGTCTCCATGGCAGGTTTTGCATATCCCTCATCATTCTCATCCAGCTTTTGCTTGAGAAACTGTTGGGCAAGACTACCTACCATTCCCAGTATCCCACCATGTTCACCTTCACCATCTCCTTGGAGTTTGTCCAGGATAACCTTCATAAACTTCTTCACTTTGGGGCTAGCATCTTCCCCAAAAACATCAAAAAGGGTAGGTCTCAGTTTACCAACATCTATATCATCTTTTCCAGTTTTCTGCTTGAGTATCTCAATGAGGGTTGAGAGTGGCAGAGACTTACTCCTTAGATAACCATAGTTGCCATGTGCGAGTTCGCTGTCCCCATCTTCTTCATCCCTGTGGTTATGACGACGCAATCCTGAAGGGACGTGAAATCCCCTGGACTCAATGGCATCCTCAACTTTCCTATGCAGAAAGCTTGAGAATCCAAAGCCAGATTCAGATTGTTCCTCACCTCCTTTAGTGCTCTCTCCTATCTGCTCCTTAGCTTCTTCAATTAAGCCACCACTATGGCAAGAATCAGATACAATTGTCAGCCTACATCCTCTAGGTATCCCATTCACAAATTCCTTGAAATCATCATCAGTGATGAGATTCATATCAGAAGGAACGATACATTCATCAAAACCAGTATCATCATCCTCTCCCGTTTCAGCAGGAAGACGCGTACCATGTCCACTATAATGAACGAACAACACATCTCCTTCCTCCGCCGATCGGATCAACTCCGACAGCACACGGCGTATGTTCTTTCCCGTCGGCTCCGTATACGACGAATCGGTGTCGATCAAAACGGTGATGTCGTCTTCGGAAAATCCGTATCGTTCGATCAAGCATTTGTGCATCCTCCATACGTCGTTTACGCATCCTCTTAACTCTGCTTTTGTTCCGGGATAGTTGATCCCAATCAACACAGCTTTTTTCGCCATTTGATTACAGAGTCTTTTGTTGGATCTAAAGAAGAAGATGAAGAAAAAAAAAGAAGTAGTTATTTGACTATTTTCTTGCTTTCTTTTTTAGCTATGATTGATTGATACACCAAAACTGTCATTTGTCATTCTATCATCTTGTGAATTTATTATTATTATTTTTTAT
TARGET=1610,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03379 Ps_contig_mira-and-tgicl-ass_franssen_29804
SEQUENCE=TTTGCTAGACCTGGATCAGAGGAATCCGAAGAAGATAACAACTTGATGCCTTCTCATAAAAGCTATTGAGAAGATCCATCAGAAGAAACAGAGCCAAAATCACCATTAACTGAATCAACGCAAGCCCAATTAGAGATACATTATAACATTAGAGCATACACACATCAATTTGTCTCACTTTTATTTTGAAAACATTGTATTCCTCAAAGCATGGGAGTTTCTGCCACATTTCAACAAAACAGTCCCTTAAAAGAAACTGAAGTTTCATATCCCTTAAAACAAATTTGACAAACAAACAAACAACCTAAGCAGAAAACTTTTCTCTGTCATGAGGAGCAGAATAATCGACATTTGGCCCCACGGGAACGATCCCAAATGGATTGATAGATGGATGACTTCCATAGTAATGTAACTTTATATGTTCCATGTTCACTGTTCTACTAATGCCAGGAACTTGGAAAATGTCTTTGGTATAGTTGAAGATATTCGGGTATTCCCGCAGTAGCTTCTTGTTACACTTAAAGTGAACTGCATAAACCTCGTCAAATCGAATGAGCGTGACAAACAAACGAATGTCTGCTTCGGTGAGTTTGTCGCCACATATATAGCGTTGCTTGCTTAGTATATCCTCGCATCTGTCCAAAGCTTCGTATAATCGTCTAACAGCATCGATGTATGGCTCCTGTTTCTTTGCGAATCCACATTTATAAACACCGTTGTTTATATCATGATATGTCCACTCATTCGTTTCATCAATTTGGGCTTTCAATTCAGTGGGATACAAGTCCAGGGCGGGATTCTCCGCGATATCGTTGAATTCAGTATTAAACATGCGGATTATCTCCGAGCTTTCGTTGTTAACAATTGTCTTGAGTTTCTTATCCCACAGAATCGGAACAGTGAACTTTCCGGTGTAGTTTGTACTTGCGATTTCGTAAAGTTCTCTAACACTCTTTGCTCCGTTCAAGGGATCGGGTTCAGCTCCAAGAACCTCGGTATTCGATTCAGGAAAAACCCATCCCACATGTTCATCTGATTCTTTCGTTATTCCCCATATCGGTTTAACCGCCGAGAAACTGATGGCCTTGTCGAGTCCTTTGATCTGCAAATAAGCTAGGCATCTGCAAGCCCAAGGACAAGCATATGATATGTAGAGATGATACCTTCCAGACTCAGGTGGAAATTGGGAATTAAGATCCCTTGAAACGAACTGTCGGAATGTGGAAGCAGATCTTGTGAATGCTCCCGAGTCTGATATCTCATCCAACGAAGATCGCGCCATTTCAACAATCTTCTAATGCTTGTAGTTACAGGTGAAGCGATGGGAGAAGAAAGAGAGGGTAAAGGAGCTTTTGGGGAGGAGCAGTGATCTTGGACAGAACATGCTTTATGTAAAATACAAGTAAAGTAAAGTTCCGATTCCGAAACAAACAAACC
TARGET=288,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0348
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=TATTCCTCAAAGCATGGGAGTT
PRIMER_RIGHT_SEQUENCE=TTTCCAAGTTCCTGGCATTAGT
PRIMER_LEFT=197,22
PRIMER_RIGHT=460,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.0437
PRIMER_LEFT_1_PENALTY=0.003166
PRIMER_RIGHT_1_PENALTY=0.040562
PRIMER_LEFT_1_SEQUENCE=TCACCATTAACTGAATCAACGC
PRIMER_RIGHT_1_SEQUENCE=CTATCAATCCATTTGGGATCGT
PRIMER_LEFT_1=99,22
PRIMER_RIGHT_1=386,22
PRIMER_LEFT_1_TM=60.003
PRIMER_RIGHT_1_TM=60.041
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.0747
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.040562
PRIMER_LEFT_2_SEQUENCE=TATTCCTCAAAGCATGGGAGTT
PRIMER_RIGHT_2_SEQUENCE=CTATCAATCCATTTGGGATCGT
PRIMER_LEFT_2=197,22
PRIMER_RIGHT_2=386,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=60.041
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.1548
PRIMER_LEFT_3_PENALTY=0.154023
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=GAGTTTCTGCCACATTTCAACA
PRIMER_RIGHT_3_SEQUENCE=TTTCCAAGTTCCTGGCATTAGT
PRIMER_LEFT_3=214,22
PRIMER_RIGHT_3=460,22
PRIMER_LEFT_3_TM=60.154
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.1836
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.149516
PRIMER_LEFT_4_SEQUENCE=TATTCCTCAAAGCATGGGAGTT
PRIMER_RIGHT_4_SEQUENCE=CCAAAGACATTTTCCAAGTTCC
PRIMER_LEFT_4=197,22
PRIMER_RIGHT_4=470,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.850
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_03380 Ps_contig_mira-and-tgicl-ass_franssen_29804
SEQUENCE=TTTGCTAGACCTGGATCAGAGGAATCCGAAGAAGATAACAACTTGATGCCTTCTCATAAAAGCTATTGAGAAGATCCATCAGAAGAAACAGAGCCAAAATCACCATTAACTGAATCAACGCAAGCCCAATTAGAGATACATTATAACATTAGAGCATACACACATCAATTTGTCTCACTTTTATTTTGAAAACATTGTATTCCTCAAAGCATGGGAGTTTCTGCCACATTTCAACAAAACAGTCCCTTAAAAGAAACTGAAGTTTCATATCCCTTAAAACAAATTTGACAAACAAACAAACAACCTAAGCAGAAAACTTTTCTCTGTCATGAGGAGCAGAATAATCGACATTTGGCCCCACGGGAACGATCCCAAATGGATTGATAGATGGATGACTTCCATAGTAATGTAACTTTATATGTTCCATGTTCACTGTTCTACTAATGCCAGGAACTTGGAAAATGTCTTTGGTATAGTTGAAGATATTCGGGTATTCCCGCAGTAGCTTCTTGTTACACTTAAAGTGAACTGCATAAACCTCGTCAAATCGAATGAGCGTGACAAACAAACGAATGTCTGCTTCGGTGAGTTTGTCGCCACATATATAGCGTTGCTTGCTTAGTATATCCTCGCATCTGTCCAAAGCTTCGTATAATCGTCTAACAGCATCGATGTATGGCTCCTGTTTCTTTGCGAATCCACATTTATAAACACCGTTGTTTATATCATGATATGTCCACTCATTCGTTTCATCAATTTGGGCTTTCAATTCAGTGGGATACAAGTCCAGGGCGGGATTCTCCGCGATATCGTTGAATTCAGTATTAAACATGCGGATTATCTCCGAGCTTTCGTTGTTAACAATTGTCTTGAGTTTCTTATCCCACAGAATCGGAACAGTGAACTTTCCGGTGTAGTTTGTACTTGCGATTTCGTAAAGTTCTCTAACACTCTTTGCTCCGTTCAAGGGATCGGGTTCAGCTCCAAGAACCTCGGTATTCGATTCAGGAAAAACCCATCCCACATGTTCATCTGATTCTTTCGTTATTCCCCATATCGGTTTAACCGCCGAGAAACTGATGGCCTTGTCGAGTCCTTTGATCTGCAAATAAGCTAGGCATCTGCAAGCCCAAGGACAAGCATATGATATGTAGAGATGATACCTTCCAGACTCAGGTGGAAATTGGGAATTAAGATCCCTTGAAACGAACTGTCGGAATGTGGAAGCAGATCTTGTGAATGCTCCCGAGTCTGATATCTCATCCAACGAAGATCGCGCCATTTCAACAATCTTCTAATGCTTGTAGTTACAGGTGAAGCGATGGGAGAAGAAAGAGAGGGTAAAGGAGCTTTTGGGGAGGAGCAGTGATCTTGGACAGAACATGCTTTATGTAAAATACAAGTAAAGTAAAGTTCCGATTCCGAAACAAACAAACC
TARGET=1425,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03381 Ps_contig_mira-and-tgicl-ass_franssen_29807
SEQUENCE=ATTACTAATACATTAATCCTTGYNNNGNAACNGTAACCACCTCCTGCGTGTGAGAGAGTTAGCACCGTGACACAATGGCAGCTACCTTGCAAACTCCGCTTCATGCATCTATCTCCTCCTCTCCCGCATCATCATCATCTTCCATTCACGCAACCAGATCCCTCTCCTTCCTCCGTTCCACACCGCCACAAACTCTCCTCTTCAACTTCAAACACTCCTCCCCTCGAATTCGCTCTTCCTCCGCTGACACCGTTATCACTGAATTGGATGCTGTTTCGGCCTTCAGCCAGATCGTTCCAGATACTGTTATTTTCGATGATTTCCAAAAGTTTCCTCCAACTGCTGCTACTGTCAGTTCGTCGCTTATTTTGGGGATATGCGGTCTTCCAGACACTATTTTCAGGAATGCTGTGGAAATGGCTTTGGCTGATTCTCAATGTTACGGCCTTGAAATTCCTAATGAAAGACTATCTTGTTTTGCCACTAAGGCTTTGGTGAACGTTGGTAGTGACATGGCAAAGCTCGTCCCTGGCCGCGTTTCAACAGAAGTGGATGCGCGTCTTGCTTATGACACACATGGCATTATCAGGAAGGTGCATGACCTGTTGAAGTTGTACAATGATAGTAATGTCCCTCGTGAACGTCTGCTATTTAAAATTCCTTCAACATGGCAGGGAATAGAGGCTGCAAGATTGCTGGAATCCGAGGGCATACAGACACATTTGACTTTCGTTTACAGCTTTGCCCAAGCTGCAGCTGCTGCTCAAGCTGGTGCATCTGTGATTCAAATTTTTGTTGGTCGCGTCAGGGACTGGGCACGTACTCATTCTGATGACGAAGAGATAGAAGCTGCCAAGCTGAGGGGAGAGGATCCCGGGTTGGCATTGATCACAAAGGCTTACAATTATATTCACAAATATGGACACAAGTCAAAGTTGATGGCAGCAGCAGTTCGCAACAAGCAGGACTTATTTAGCCTTCTGGGGGTTGATTACATCATAGCTCCCTTAAAGGTGTTACAGTCTCTCAAAGAATCTGTTGCTTCTCCTGAGGAGAAGTATTCTTTTGTTAGGAGGCTATCACCTCAGTCTGCTAGCAGATACACATTTAGTGATGAAGAGCTTATTAAATGGGACCAAATGAGCCTTGCAAATGCCATGGGACCAGCAGCGGTGCAGCTTCTGACTTCTGGGCTGGACGGTTATGCCGATCAAGCAAAGAGGGTGGAAGATTTATTCGGGAAAATTTGGCCACCACCAAATGTATAACTTTACCTCTTTATGTTGCTCAATAGATGTTTACCTTGATGGAATAAACTGAACATACAACAGTTGAATGGAATCTTCATTTTGTTTTTTCGTTTCTTCCCTTGATCTCTTGTATCTATTTTCTACGTTTCCCTTCACGTAAAGCACCCGGTAACCTTAAAAAGTTAAA
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03382 Ps_contig_mira-and-tgicl-ass_franssen_29819
SEQUENCE=AANCACCATAATATTCATGTAATAAAGACAAGAATCAAAGGAAGCTTAAATGGATAGAGAGTTGCTGAGTTTGGCGGTCGTGAAGAGGCCGTGTTGCGGATCTCCAGAGTCCAGTCCTTTTGGCAGTGGGGTGTAGTTGTGCCAATACATGGTAGGCAGCTCATCTTTTGGTCTAAGGCGTGAAACTGACTTTAGAGGTTTGAGGGGAAGTGAGATGGCTGCAACTGCAAGCAATGATGTGGATGTTGTTGGGGCAGGTCCATCAGGAACCTGGTGGCTCTGGTGGGGGATGACGAAAGGCAGAAAAGCTAATGGTTGCAGTTTACTCAACAAAATTCGGAAATCATATTGAAAAGATGGAAGGAAGCATACATTATTCCACAAATGGATCATCAATCTACTCTATACAGAATAAAGTCTTTAATTGAAAATATCTAAAAATCATCCTTTGCTTGCCGGATGATACCCAGAGCTTCTGCATCATCTGCTGTGACTGCAATGTTTAATGATTTCCTGATATGTGCACTGGATGTTCGAAGGAATGGATTGCAGGCTTTTTCCAACTTCAGTATGGTTGGAATTGTGGGCAAGCCTTTACTTCGGAGGTAAGTAACATGGGCAGCATAGGATTTAAGTTCCTTGTTTTCAGGTTCGACCGATAATGCAAACTTTGCATTACTCAATGTGTACTCGTGTCCACAGTATATACTTGTATTGTCTGGTAAAGACGTGATCTTTTTAAGCGAAGATTGCATCTGTTCAGGGCTTCCTTCAAAGAGTTTGCCACATGATAAACTGAACAAAGTGTCTCCTGTAAAGATTGCCCCAGATCCCGGAACGTAGAAGCTTATGTGACCTCGAGTGTGTCCAGGTGTGTCCATTACATGCACCTCATGGCCAGCAAACATCCACTTATCACCGTCATTCAAAAGGATATCGATGCCAGGAATCCTTTCCTTGTCAGCTCCTGAACCAATAACCTTTGCCCCATATCTTGCTTTTAATTCTACATTTCCACCAGTATGATCATGATGATGGTGAGTGTTGAGTATATAATTCAAATTTCGATTTTTCTTGCTTAACGCATCTATGACAGGCAAAGCTTCAGAAGGATCAACAACTCCAACTGTGCCCGTATCAACATCGTGTAAAAGGTATGCATAATTGTCACTCAAGCAAGGTACCAATTCAATCTGCAAGGTGGAAGACATATTCGTGACACTACAAAACCGAGCCACTTTGAGCGAGCGACTAGCTCCTCCTCGCAGTGTTTTATACGGAGTAGAAAACATTCGCATAAATCCATACAGAATACTTTTTCTAAAGCAAAGTTGCCTCACATTAGGCCACACACTAAACCCAGCTCTCACCCTAGAAGAAGAAAAGGGGAGCATGGCAGATGTTGAAGGGCTTGAAAGCATGTGAATGGAGAATTGAATAGAAGGAAAGAGAAAGAGTGGGTTGGCACTAATTTTAGTGTTTGGTTGAAAATGAAATTGGGTTCTCTTCTCTCTCTCTCTTTCCTACCACCGTGTCTTCCACCACCACCTCTTCCTCTTCATCAATCCCCGAYTCATGTTTCTCTCTCCTTA
TARGET=1508,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03383 Ps_contig_mira-and-tgicl-ass_franssen_29823
SEQUENCE=AACTANCTNNGAATAGTTATCCTTTTTGTNGAAACTTCTTTGTTTCTGGTCCTTCTTTTACTTCTTCTAGTTCTGTGTATCATAAACACGCTGAAGACGAAAACAATGGCTTCCGATGACAAATCAGCGACATGACGAAAACAATGGCTTCCGATGACAAATCAGCGACATGACGAAAACAATGGCTTCCGATGACAAATCAACGACGTTTCCGAAGAATTACGGTTACGATTTGGTTTTGGGATGAATCGCTGCTTTCTACATACTCATGGTACCTTACACTAAGGTCGAAGAAAGTTTCAAATGTTCAGGCAATGCATGATATTCTTTATCATCGGTTTCACATAGATAATTTTGATTATCTGGAGTTTCCTGGCGTTGTTCCTCGCACTTTCATTGGCGCTTTGCTTGTCTCACTTGTTGCGTCTCCGATTGTGTTAATTGCAAGTTTACTGCATTTGCCAAAGTTTTATGCTCTTCTCATAGTTCGAATGGTCATAGGATGCATCATACTGTATACACTAAGATTCTTCCGTCAACAGATCAGGAATAAATTTGGGCGCCAAGTAGAAACCTTCTTTGTGATACTAACTTCCATTCAGTTTCATTTTCTGTTCTATTGTTCTCGCCCGCTTCCTAACATTCTTGCTTTGGGTATAGTGAATTTGGCGTTTGGGTACTGGTTCCAGGGGCGCTTTTTATGCTGCTCTAAACTCTTTGGTATGTATGTCATATTTTACTTCAGTTTCATGTTGGGGCAGTATTTTTTAATTTTCCTTTACTAGCTTTTATGCTAATTTTTCCCACTTCAAAACAATGTACATTACTTTTTGCAATGCTATTATTATTATTTAATTAAATCTCCTGTTCGAGAATTAGTTTCCAAATTACACTGTAAAGAATTTAAATTTTGGATCTTAATTAGGAGGTGGGGACATAGAAATTAAG
TARGET=840,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0372
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.036444
PRIMER_LEFT_SEQUENCE=TCCTAACATTCTTGCTTTGGGT
PRIMER_RIGHT_SEQUENCE=CCCCACCTCCTAATTAAGATCC
PRIMER_LEFT=634,22
PRIMER_RIGHT=933,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.036
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.1522
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.036444
PRIMER_LEFT_1_SEQUENCE=GTTTCATGTTGGGGCAGTATTT
PRIMER_RIGHT_1_SEQUENCE=CCCCACCTCCTAATTAAGATCC
PRIMER_LEFT_1=744,22
PRIMER_RIGHT_1=933,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=60.036
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.1910
PRIMER_LEFT_2_PENALTY=0.115769
PRIMER_RIGHT_2_PENALTY=0.075241
PRIMER_LEFT_2_SEQUENCE=GTTTCATGTTGGGGCAGTATTT
PRIMER_RIGHT_2_SEQUENCE=AATTTCTATGTCCCCACCTCCT
PRIMER_LEFT_2=744,22
PRIMER_RIGHT_2=944,22
PRIMER_LEFT_2_TM=60.116
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.2685
PRIMER_LEFT_3_PENALTY=0.232060
PRIMER_RIGHT_3_PENALTY=0.036444
PRIMER_LEFT_3_SEQUENCE=AGTTTCATGTTGGGGCAGTATT
PRIMER_RIGHT_3_SEQUENCE=CCCCACCTCCTAATTAAGATCC
PRIMER_LEFT_3=743,22
PRIMER_RIGHT_3=933,22
PRIMER_LEFT_3_TM=59.768
PRIMER_RIGHT_3_TM=60.036
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=0.2863
PRIMER_LEFT_4_PENALTY=0.115769
PRIMER_RIGHT_4_PENALTY=0.170538
PRIMER_LEFT_4_SEQUENCE=GTTTCATGTTGGGGCAGTATTT
PRIMER_RIGHT_4_SEQUENCE=TTTCTATGTCCCCACCTCCTAA
PRIMER_LEFT_4=744,22
PRIMER_RIGHT_4=942,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=59.829
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_03384 Ps_contig_mira-and-tgicl-ass_franssen_29828
SEQUENCE=AACTTTAAAATTTTACTTTTATTCATCAAAATTGCAACATAAAAAAGTCAACTTATACTTAAAAAAGATCACTACCACAAAGTAAAGAAGAAAAAATAGCATTTCTATTACTACAGCTGCCCCCTGATTGCTTATGCTTAACAGACCACGCAAGAGAACAATTGTTTCACCTTACAGTGACATATAACATTGGACCTCAAACAAAAATGAAAAACTGAATCTTCAACTATTTAACACTGCTCCAAGTAGGGTTGCTGAATCTTATTATTATGCTTAGAGTAGTACTTCCCTGTCGTGAATCCAACTGAATCGATGTTTTGATGATTCCTTTGCTCTTGCTAGAGCTCTCTCCTCCAGCCTTCTGATTCTTGGTGGCAACCCACAAACATACTCCTGTGCCTTTCGCCCCTCACCTGAAAGTCCGGTTATGTCCGCCACCTTCCACCTCCCAACCAGAAACTCAAGTATATCAGCATAGTCCTTGGCAGTGTAGACCCCAATGCGCTGGGCAACGGCAGAGTAGTTATCAAACAGGTTGTCGTCTCGCCCGTCATACATCAGATGTGCTGGCATAGCAATTTTCTTCCTCATCATGTCAGCAAAGGCCATAACTGTTCCGTCAGGATCAATCTCAAACAGCTTTTCCACTATCTTTGTGTAAGCAGTCTCGTGGCGTTTTTCATCCGAGGCAATCAGACCACAGATCTGAGCCAATTTCAAATCACCGCGTTCTTTAGCAAGCCTGGCTGTGTTTCCGTGCGATATAAAGGTTGCCCTCTCTTGAAATGAAGTGTAAATGAAACCAAGGTAGGGGCTATTCTCAGTCCGAGGATCCATTCCAGAGCCAATCAAGTACTGAATTGTCTTCTCAATTTGTCTCATATCAACACGGCCAGACAAGTAAAGATACTTGTTAAGAAGATCACCGTGTCTATTTTCTTCAGCTGTCCATGCCCTTGTCCAAACAGCCCAAGGAGTAGGGCTGGCACCTGTTTCATCACGAACTCCGTCCAAAGTATTAAGCATTGTTTGGTAAGTAGGAAGGGCTTCTTCTGTGACCATATCACCAACCAGAACAACAAAGTAATCATCTGGAAGCTCTTTAGCTCTCTCTCTCAGCTCCTTCACTTGCTCTTCGAATCCATCTGACGAAGGATCCGGCAGAAAATCCTGTGGTTGCCAACACTTTTCTACTGGCTTAAGGTGAGTCAATAAGGTTGTCTCAGCCCAACCCTCTAAAGATTTGAAAATTTCAATCTTCTGGGGAGGCATAGAGTGGGTTACTTGAACATGCACTTCTCTGGGAGGCGTGAAAGCCTTCTTAATATTTTCAACCTCTTTGGAACCGGAGCGGAGGGTGGAAGCCATGACGAATTTGGGAGATCTGAGACTGGCCATTTGAGGGAGGGAGAAGGAGGAAGTTTGAGTAGGGAAAGGGGTTGTTACTCGGAGAGCCATTGTTGGATTCGATTGAAGAATTTTGTGTTTTTAGGGAATAACGCGGATTGAGAAAGAGAAAGAGAAAGAGAGAGAGAGTGTGTATGTGTATTCGTGTATTTGTGTATTGTGATGTGAGGCCACCG
TARGET=1107,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0361
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=GGGCTTCTTCTGTGACCATATC
PRIMER_RIGHT_SEQUENCE=GGGCTGAGACAACCTTATTGAC
PRIMER_LEFT=1043,22
PRIMER_RIGHT=1228,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.5000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=186
PRIMER_PAIR_PENALTY_1=0.0691
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=GGGCTTCTTCTGTGACCATATC
PRIMER_RIGHT_1_SEQUENCE=TGCATGTTCAAGTAACCCACTC
PRIMER_LEFT_1=1043,22
PRIMER_RIGHT_1=1294,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.0797
PRIMER_LEFT_2_PENALTY=0.060824
PRIMER_RIGHT_2_PENALTY=0.018835
PRIMER_LEFT_2_SEQUENCE=ACACGGCCAGACAAGTAAAGAT
PRIMER_RIGHT_2_SEQUENCE=ATTCGAAGAGCAAGTGAAGGAG
PRIMER_LEFT_2=886,22
PRIMER_RIGHT_2=1140,22
PRIMER_LEFT_2_TM=60.061
PRIMER_RIGHT_2_TM=60.019
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=0.1188
PRIMER_LEFT_3_PENALTY=0.114470
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=GTAGGAAGGGCTTCTTCTGTGA
PRIMER_RIGHT_3_SEQUENCE=GGGCTGAGACAACCTTATTGAC
PRIMER_LEFT_3=1036,22
PRIMER_RIGHT_3=1228,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.1188
PRIMER_LEFT_4_PENALTY=0.114470
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=TAGGAAGGGCTTCTTCTGTGAC
PRIMER_RIGHT_4_SEQUENCE=GGGCTGAGACAACCTTATTGAC
PRIMER_LEFT_4=1037,22
PRIMER_RIGHT_4=1228,22
PRIMER_LEFT_4_TM=59.886
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_03385 Ps_contig_mira-and-tgicl-ass_franssen_29828
SEQUENCE=AACTTTAAAATTTTACTTTTATTCATCAAAATTGCAACATAAAAAAGTCAACTTATACTTAAAAAAGATCACTACCACAAAGTAAAGAAGAAAAAATAGCATTTCTATTACTACAGCTGCCCCCTGATTGCTTATGCTTAACAGACCACGCAAGAGAACAATTGTTTCACCTTACAGTGACATATAACATTGGACCTCAAACAAAAATGAAAAACTGAATCTTCAACTATTTAACACTGCTCCAAGTAGGGTTGCTGAATCTTATTATTATGCTTAGAGTAGTACTTCCCTGTCGTGAATCCAACTGAATCGATGTTTTGATGATTCCTTTGCTCTTGCTAGAGCTCTCTCCTCCAGCCTTCTGATTCTTGGTGGCAACCCACAAACATACTCCTGTGCCTTTCGCCCCTCACCTGAAAGTCCGGTTATGTCCGCCACCTTCCACCTCCCAACCAGAAACTCAAGTATATCAGCATAGTCCTTGGCAGTGTAGACCCCAATGCGCTGGGCAACGGCAGAGTAGTTATCAAACAGGTTGTCGTCTCGCCCGTCATACATCAGATGTGCTGGCATAGCAATTTTCTTCCTCATCATGTCAGCAAAGGCCATAACTGTTCCGTCAGGATCAATCTCAAACAGCTTTTCCACTATCTTTGTGTAAGCAGTCTCGTGGCGTTTTTCATCCGAGGCAATCAGACCACAGATCTGAGCCAATTTCAAATCACCGCGTTCTTTAGCAAGCCTGGCTGTGTTTCCGTGCGATATAAAGGTTGCCCTCTCTTGAAATGAAGTGTAAATGAAACCAAGGTAGGGGCTATTCTCAGTCCGAGGATCCATTCCAGAGCCAATCAAGTACTGAATTGTCTTCTCAATTTGTCTCATATCAACACGGCCAGACAAGTAAAGATACTTGTTAAGAAGATCACCGTGTCTATTTTCTTCAGCTGTCCATGCCCTTGTCCAAACAGCCCAAGGAGTAGGGCTGGCACCTGTTTCATCACGAACTCCGTCCAAAGTATTAAGCATTGTTTGGTAAGTAGGAAGGGCTTCTTCTGTGACCATATCACCAACCAGAACAACAAAGTAATCATCTGGAAGCTCTTTAGCTCTCTCTCTCAGCTCCTTCACTTGCTCTTCGAATCCATCTGACGAAGGATCCGGCAGAAAATCCTGTGGTTGCCAACACTTTTCTACTGGCTTAAGGTGAGTCAATAAGGTTGTCTCAGCCCAACCCTCTAAAGATTTGAAAATTTCAATCTTCTGGGGAGGCATAGAGTGGGTTACTTGAACATGCACTTCTCTGGGAGGCGTGAAAGCCTTCTTAATATTTTCAACCTCTTTGGAACCGGAGCGGAGGGTGGAAGCCATGACGAATTTGGGAGATCTGAGACTGGCCATTTGAGGGAGGGAGAAGGAGGAAGTTTGAGTAGGGAAAGGGGTTGTTACTCGGAGAGCCATTGTTGGATTCGATTGAAGAATTTTGTGTTTTTAGGGAATAACGCGGATTGAGAAAGAGAAAGAGAAAGAGAGAGAGAGTGTGTATGTGTATTCGTGTATTTGTGTATTGTGATGTGAGGCCACCG
TARGET=1525,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4165
PRIMER_LEFT_PENALTY=0.109273
PRIMER_RIGHT_PENALTY=0.307272
PRIMER_LEFT_SEQUENCE=GGGAGAAGGAGGAAGTTTGAGT
PRIMER_RIGHT_SEQUENCE=GTGGCCTCACATCACAATACAC
PRIMER_LEFT=1406,22
PRIMER_RIGHT=1580,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.307
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=175
PRIMER_PAIR_PENALTY_1=0.4418
PRIMER_LEFT_1_PENALTY=0.134571
PRIMER_RIGHT_1_PENALTY=0.307272
PRIMER_LEFT_1_SEQUENCE=TGAGTAGGGAAAGGGGTTGTTA
PRIMER_RIGHT_1_SEQUENCE=GTGGCCTCACATCACAATACAC
PRIMER_LEFT_1=1423,22
PRIMER_RIGHT_1=1580,22
PRIMER_LEFT_1_TM=59.865
PRIMER_RIGHT_1_TM=60.307
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=0.5395
PRIMER_LEFT_2_PENALTY=0.232197
PRIMER_RIGHT_2_PENALTY=0.307272
PRIMER_LEFT_2_SEQUENCE=AGGGAGAAGGAGGAAGTTTGAG
PRIMER_RIGHT_2_SEQUENCE=GTGGCCTCACATCACAATACAC
PRIMER_LEFT_2=1405,22
PRIMER_RIGHT_2=1580,22
PRIMER_LEFT_2_TM=60.232
PRIMER_RIGHT_2_TM=60.307
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=176
PRIMER_PAIR_PENALTY_3=0.6406
PRIMER_LEFT_3_PENALTY=0.333282
PRIMER_RIGHT_3_PENALTY=0.307272
PRIMER_LEFT_3_SEQUENCE=GAAGTTTGAGTAGGGAAAGGGG
PRIMER_RIGHT_3_SEQUENCE=GTGGCCTCACATCACAATACAC
PRIMER_LEFT_3=1417,22
PRIMER_RIGHT_3=1580,22
PRIMER_LEFT_3_TM=60.333
PRIMER_RIGHT_3_TM=60.307
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.6406
PRIMER_LEFT_4_PENALTY=0.333282
PRIMER_RIGHT_4_PENALTY=0.307272
PRIMER_LEFT_4_SEQUENCE=GGAAGTTTGAGTAGGGAAAGGG
PRIMER_RIGHT_4_SEQUENCE=GTGGCCTCACATCACAATACAC
PRIMER_LEFT_4=1416,22
PRIMER_RIGHT_4=1580,22
PRIMER_LEFT_4_TM=60.333
PRIMER_RIGHT_4_TM=60.307
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_03386 Ps_contig_mira-and-tgicl-ass_franssen_29830
SEQUENCE=GTTGGAAATTTGGGGAAAGGGAAAACAAAGGTGAAATATGAACTTGACAAAAAAACCGGAACTTATTAAGGTTGACCGTGTGCTTTACTCATCAGTTGTGTATCCTCACAACTATGGGTTTTGTCCCCCGCACTATTTGTGAGGATGGTGATCCCATCGATGTCTTGGTCATTATGCAGGAGCCAGTTCTTCCAGGATGCTTTCTTCGGGCCAAAGCTATTGGACTCATGCCTATGATTGATCAGGGTGAGAAAGATGACAAGATAATTGCTGTCTGTGCTGATGATCCCGAGTATAGGCATTTCAATGATATCAAGGAGCTCCCTCCACATCGTTTAGCTGAAATTCGTCGTTTTTTTGAAGATTACAAGAAGAACGAGAACAAGGAAGTTGCAGTAAATGACTTTCTCCCTGCCTCATCTGCCTATGAAGCGATCGAGCATTCCATGACCTTGTATGCGGACTACGTGGTGGAGAGCTTGAGGCGGTAGCTTTGATGGATATGGGATTTTGTTTGGTCGTGAAGATATATTTTGAAGGCTGCCATGCCATGCCATTTTATAACTAAGATTACGACTGAAAAAGATATTTTGTATCGTATTACTGTCATATTCCTATAGCATGTACATTTGTCTCACAAAGGACAAGTAACTACACATGCATATCCTACACACATATATTTTGTCTTGTACTTTTTTAANTTTTAAATTGNTATATTCGTTTATTAATTATTTCTAAACTTACTTGTTTTTAATTTATTACTTATTTTTTAAACCG
TARGET=542,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.5590
PRIMER_LEFT_PENALTY=0.071684
PRIMER_RIGHT_PENALTY=3.487288
PRIMER_LEFT_SEQUENCE=AGAACGAGAACAAGGAAGTTGC
PRIMER_RIGHT_SEQUENCE=TGCATGTGTAGTTACTTGTCCTTTG
PRIMER_LEFT=372,22
PRIMER_RIGHT=661,25
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=60.487
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=290
PRIMER_PAIR_PENALTY_1=3.6580
PRIMER_LEFT_1_PENALTY=0.170703
PRIMER_RIGHT_1_PENALTY=3.487288
PRIMER_LEFT_1_SEQUENCE=GGTAGCTTTGATGGATATGGGA
PRIMER_RIGHT_1_SEQUENCE=TGCATGTGTAGTTACTTGTCCTTTG
PRIMER_LEFT_1=486,22
PRIMER_RIGHT_1=661,25
PRIMER_LEFT_1_TM=60.171
PRIMER_RIGHT_1_TM=60.487
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=3.7620
PRIMER_LEFT_2_PENALTY=0.274705
PRIMER_RIGHT_2_PENALTY=3.487288
PRIMER_LEFT_2_SEQUENCE=TAAATGACTTTCTCCCTGCCTC
PRIMER_RIGHT_2_SEQUENCE=TGCATGTGTAGTTACTTGTCCTTTG
PRIMER_LEFT_2=396,22
PRIMER_RIGHT_2=661,25
PRIMER_LEFT_2_TM=59.725
PRIMER_RIGHT_2_TM=60.487
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=3.8307
PRIMER_LEFT_3_PENALTY=0.343387
PRIMER_RIGHT_3_PENALTY=3.487288
PRIMER_LEFT_3_SEQUENCE=ACGAGAACAAGGAAGTTGCAGT
PRIMER_RIGHT_3_SEQUENCE=TGCATGTGTAGTTACTTGTCCTTTG
PRIMER_LEFT_3=375,22
PRIMER_RIGHT_3=661,25
PRIMER_LEFT_3_TM=60.343
PRIMER_RIGHT_3_TM=60.487
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=3.9074
PRIMER_LEFT_4_PENALTY=0.420066
PRIMER_RIGHT_4_PENALTY=3.487288
PRIMER_LEFT_4_SEQUENCE=GATATGGGATTTTGTTTGGTCG
PRIMER_RIGHT_4_SEQUENCE=TGCATGTGTAGTTACTTGTCCTTTG
PRIMER_LEFT_4=499,22
PRIMER_RIGHT_4=661,25
PRIMER_LEFT_4_TM=60.420
PRIMER_RIGHT_4_TM=60.487
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_03387 Ps_contig_mira-and-tgicl-ass_franssen_29833
SEQUENCE=CTTATTTTATTTTTTTCCTTTTTTATTACCACTTNCTCTCTATGAATACCCTTGGAACTCTCTCTTTTTCTTCTCTATCCATCTCTTTGTTTTCTCAGACCCAAAATTTTCTTTGGTTCATTTTTTTCCCTCTTCTCCTCTTAGAGGAGTCTCTGATTCAGCTTACCTCAGGTGTTCTTTGTTAAGATCTTTGGTTTTCAGGTGTGAAAATTGAGAGAAAGATGAATCACTGTGGTTACCATCAGAAGAACGCCATGAGAAGCTGTGAAGAGATGAGGATGGAATCTGTGGTTTGCCCTAAGCCTCGTAGATTTGGTCTGATGAACAACTCATTTATTAACAACAACAACAACCATATCAGACCCTATAAGCCACAAATTATCAGCTACCAATCAGAGCTTGAAGATTCAGGTGTCAGGGAAGATCTTCTTGATATCATTCTCCCCAAGGGAAATAGCTATATGGAAAGATTTGGGGACCAAATGGCATCATCACCACCACCATTTTTTTGTGGATCTCCACCTAGCAGGGCTTCAAACCCTGTGATCCAAGATGAGCAATTTGGCAATGGCAATGGTCATGGAAATTTTAGTCCATTTTCCATGGCACCCTCTTCACCATCTTCCTCAGCTAGAAGCTGTGTTCCAATGAAGTTTGGTCACAAACAAGCTGCAGTGAGGATTGAAGGTTTTGATTGTCTCAATAGGGATAGGCGTAACCGCAGTATTTCAGCTGTTGCATAATTCATTTCCAACAAAAGAAAAACAAGTTCAAAGAGCAAGTGAAGATTTTTGGTGTCAAAGGAAGCTAATCTTAATTCAATGTTTTATTGTAATATACTAGTATATGTATATAAAAATGGTCTGTGGAAAAATTGTTAATGAGTTAAGGTGTCATGTAAAATTAGGGATGGAAAGTGAACTTTGGTCATGTTGTAGTTTGTAGTAGTTATTTGAAATGTTGTAATGGTCTATGTAATTGGAAATGTATCTATGCAATTCATGGTTTTTACTACATTGAATAAAAAAAAGTAAAATTTTRGG
TARGET=339,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03388 Ps_contig_mira-and-tgicl-ass_franssen_29839
SEQUENCE=GCGGTATCAACGGAGAGTNCGCGGGGAACCACGATTTTCTCTTCATTCATTCATCCAACAAAACTTCCCAAATCAGAGAGAGATATCTTCATCGATCAACCTTCTGAATCACGTACCGTGGAAAGAGACACTAATCAAAATTTGAACAACAATTCATCAAAATGTCCTCCCTTGCAGCTGCTAGAGCAGATAACTTTTACTATCCCCCAGAATGGGATCCAAGCCAGGGTTCTCTGAACACGTTCCATGGCCAACATGCCTTAAGAGAGAGAGCAAGGAAGATAGATCAGGGTATCTTGATTATAAGGTTTGAGATGCCTTATAATATATGGTGTGGAGGATGCAATTCTATGATTGCAAAGGGTGTCCGGTTCAATGCAGAGAAGAAGCAAGTTGGGAATTACTATTCTACAAAGATATGGAGCTTTACCATGAAAGCTGCATGCTGTAGACAAGAGATCACCATCCAGACTGATCCAAAGAACTGTGAGTATGTGATCATCAGTGGGGCTCAGAAAAAAACTGAGGACTTTGATGTTGAGGATGCAGAAACTTTTGAATTACCTGCTGATGAAGAAAAAGGGAAGCTTGCTGATCCATTTTATCGCCTCGAACACCAGGAAGCAGACTTGAAGAAAAAGAAAGAAGCTGAACCAGTGCTTGTTCGTCTCCAGCGAATGTCTGATGCCAGACATTCGGATGACTATGCCATAAATAAGAGTCTCAGGGCCAAACTTAGAAGTCAAAAGAAAAGAGTTGCTGAAGAAGAAGCTGCATCTAGGAAAAGGGGCCTTGGAATTCGACTACTTCCAGCTTCTGAACAAGATGCTGCCACAGCAGCAAAAGTGAAGTTTTCTAGTAAGTTTGAAAAAAATAGGAAGGACAAACGGGCATTGATCAACTCTGGTTCCATATTCCCAGGATTGTCTGCCTCATCCATGTCTAGTAAGAGAAGGCTAGAGCTAGAGTCTAAAAGAAGAAAAATTTCTGCAAGTGCGGCATCTAGCCTTTTGGCTGGGGGATATAAACCGTCATCATGGTCAGAGGGCGCTGTTTTGTCAAGCAGGCAAAAGGGAGCTTCAATGAATTCAAGACACTAGAAAGCTTAAAAATAAAAATGTCACGGTTGATTTTGGCTTATTGGCTCAGACATGTACATAAAAATTGCGTAATAAATGTTATTTGCTTATGTAGTTGTTTGTATTGGGAGCACAAGATAGATGGGAAGTATATATGATAATTTTGTATTCTATAGTGTGAGCCATATATGATGTGTAATGGAAGTCGGTTAATTACCTATTTCAAACTAAAGTTAGTAAATTAAAGTTAAAACGGAAAAAAAAACC
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.4513
PRIMER_LEFT_PENALTY=2.379605
PRIMER_RIGHT_PENALTY=0.071684
PRIMER_LEFT_SEQUENCE=GCGGTATCAACGGAGAGTNC
PRIMER_RIGHT_SEQUENCE=ACGGTACGTGATTCAGAAGGTT
PRIMER_LEFT=0,20
PRIMER_RIGHT=118,22
PRIMER_LEFT_TM=60.380
PRIMER_RIGHT_TM=59.928
PRIMER_LEFT_GC_PERCENT=57.895
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.50
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7500
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=2.4513
PRIMER_LEFT_1_PENALTY=2.379605
PRIMER_RIGHT_1_PENALTY=0.071684
PRIMER_LEFT_1_SEQUENCE=GGTATCAACGGAGAGTNCGC
PRIMER_RIGHT_1_SEQUENCE=ACGGTACGTGATTCAGAAGGTT
PRIMER_LEFT_1=2,20
PRIMER_RIGHT_1=118,22
PRIMER_LEFT_1_TM=60.380
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=57.895
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.75
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.5500
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=2.6990
PRIMER_LEFT_2_PENALTY=2.306329
PRIMER_RIGHT_2_PENALTY=0.392624
PRIMER_LEFT_2_SEQUENCE=GGGGAACCACGATTTTCTCT
PRIMER_RIGHT_2_SEQUENCE=CTCTCTCTTAAGGCATGTTGGC
PRIMER_LEFT_2=22,20
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=60.306
PRIMER_RIGHT_2_TM=60.393
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=2.7299
PRIMER_LEFT_3_PENALTY=2.306329
PRIMER_RIGHT_3_PENALTY=0.423592
PRIMER_LEFT_3_SEQUENCE=GGGGAACCACGATTTTCTCT
PRIMER_RIGHT_3_SEQUENCE=GTCTCTTTCCACGGTACGTGAT
PRIMER_LEFT_3=22,20
PRIMER_RIGHT_3=128,22
PRIMER_LEFT_3_TM=60.306
PRIMER_RIGHT_3_TM=60.424
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=2.7722
PRIMER_LEFT_4_PENALTY=2.379605
PRIMER_RIGHT_4_PENALTY=0.392624
PRIMER_LEFT_4_SEQUENCE=GCGGTATCAACGGAGAGTNC
PRIMER_RIGHT_4_SEQUENCE=CTCTCTCTTAAGGCATGTTGGC
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=270,22
PRIMER_LEFT_4_TM=60.380
PRIMER_RIGHT_4_TM=60.393
PRIMER_LEFT_4_GC_PERCENT=57.895
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.50
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7500
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_03389 Ps_contig_mira-and-tgicl-ass_franssen_29839
SEQUENCE=GCGGTATCAACGGAGAGTNCGCGGGGAACCACGATTTTCTCTTCATTCATTCATCCAACAAAACTTCCCAAATCAGAGAGAGATATCTTCATCGATCAACCTTCTGAATCACGTACCGTGGAAAGAGACACTAATCAAAATTTGAACAACAATTCATCAAAATGTCCTCCCTTGCAGCTGCTAGAGCAGATAACTTTTACTATCCCCCAGAATGGGATCCAAGCCAGGGTTCTCTGAACACGTTCCATGGCCAACATGCCTTAAGAGAGAGAGCAAGGAAGATAGATCAGGGTATCTTGATTATAAGGTTTGAGATGCCTTATAATATATGGTGTGGAGGATGCAATTCTATGATTGCAAAGGGTGTCCGGTTCAATGCAGAGAAGAAGCAAGTTGGGAATTACTATTCTACAAAGATATGGAGCTTTACCATGAAAGCTGCATGCTGTAGACAAGAGATCACCATCCAGACTGATCCAAAGAACTGTGAGTATGTGATCATCAGTGGGGCTCAGAAAAAAACTGAGGACTTTGATGTTGAGGATGCAGAAACTTTTGAATTACCTGCTGATGAAGAAAAAGGGAAGCTTGCTGATCCATTTTATCGCCTCGAACACCAGGAAGCAGACTTGAAGAAAAAGAAAGAAGCTGAACCAGTGCTTGTTCGTCTCCAGCGAATGTCTGATGCCAGACATTCGGATGACTATGCCATAAATAAGAGTCTCAGGGCCAAACTTAGAAGTCAAAAGAAAAGAGTTGCTGAAGAAGAAGCTGCATCTAGGAAAAGGGGCCTTGGAATTCGACTACTTCCAGCTTCTGAACAAGATGCTGCCACAGCAGCAAAAGTGAAGTTTTCTAGTAAGTTTGAAAAAAATAGGAAGGACAAACGGGCATTGATCAACTCTGGTTCCATATTCCCAGGATTGTCTGCCTCATCCATGTCTAGTAAGAGAAGGCTAGAGCTAGAGTCTAAAAGAAGAAAAATTTCTGCAAGTGCGGCATCTAGCCTTTTGGCTGGGGGATATAAACCGTCATCATGGTCAGAGGGCGCTGTTTTGTCAAGCAGGCAAAAGGGAGCTTCAATGAATTCAAGACACTAGAAAGCTTAAAAATAAAAATGTCACGGTTGATTTTGGCTTATTGGCTCAGACATGTACATAAAAATTGCGTAATAAATGTTATTTGCTTATGTAGTTGTTTGTATTGGGAGCACAAGATAGATGGGAAGTATATATGATAATTTTGTATTCTATAGTGTGAGCCATATATGATGTGTAATGGAAGTCGGTTAATTACCTATTTCAAACTAAAGTTAGTAAATTAAAGTTAAAACGGAAAAAAAAACC
TARGET=264,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3109
PRIMER_LEFT_PENALTY=0.095629
PRIMER_RIGHT_PENALTY=0.215225
PRIMER_LEFT_SEQUENCE=ACGTACCGTGGAAAGAGACACT
PRIMER_RIGHT_SEQUENCE=ATAGAATTGCATCCTCCACACC
PRIMER_LEFT=110,22
PRIMER_RIGHT=351,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=60.215
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.4018
PRIMER_LEFT_1_PENALTY=0.186600
PRIMER_RIGHT_1_PENALTY=0.215225
PRIMER_LEFT_1_SEQUENCE=TCTTCATCGATCAACCTTCTGA
PRIMER_RIGHT_1_SEQUENCE=ATAGAATTGCATCCTCCACACC
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=59.813
PRIMER_RIGHT_1_TM=60.215
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.5645
PRIMER_LEFT_2_PENALTY=0.095629
PRIMER_RIGHT_2_PENALTY=0.468913
PRIMER_LEFT_2_SEQUENCE=ACGTACCGTGGAAAGAGACACT
PRIMER_RIGHT_2_SEQUENCE=TCATAGAATTGCATCCTCCACA
PRIMER_LEFT_2=110,22
PRIMER_RIGHT_2=353,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.469
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.6383
PRIMER_LEFT_3_PENALTY=0.095629
PRIMER_RIGHT_3_PENALTY=0.542697
PRIMER_LEFT_3_SEQUENCE=ACGTACCGTGGAAAGAGACACT
PRIMER_RIGHT_3_SEQUENCE=CATAGAATTGCATCCTCCACAC
PRIMER_LEFT_3=110,22
PRIMER_RIGHT_3=352,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.457
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.6388
PRIMER_LEFT_4_PENALTY=0.423592
PRIMER_RIGHT_4_PENALTY=0.215225
PRIMER_LEFT_4_SEQUENCE=ATCACGTACCGTGGAAAGAGAC
PRIMER_RIGHT_4_SEQUENCE=ATAGAATTGCATCCTCCACACC
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=351,22
PRIMER_LEFT_4_TM=60.424
PRIMER_RIGHT_4_TM=60.215
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_03390 Ps_contig_mira-and-tgicl-ass_franssen_29845
SEQUENCE=GGCTAATTTCCATTTTGTTAGTTCGGTTTAGGTGCAGAGAGAGAGAGTGTTGAGATTTTTTCTTTCATTTTTCTTATTAATTTCTCTTTATTTGTTAGGTGTTTTTTCTCTTTCCTCATTTTTGTAGGTATATTGTTGAAGAAGACTTTGTAGCTTGACTTTGTTCAAAAGCATACGATTCAGGGAAGGCCGTAAAGTTGGAGTGAAATTAGATTGAAATCTTAGAAGTTTCTCTCTTTGGATTTGAAGAAATGGAGGTATATGGAAATTCTATGGTTGCCGCTCCTTCAAATGTTATTTATCTGTCAAGTATTTTGGGCCATGATGGTCCGAGTTCTGTTCACAAATGCGACTGGAAATGCCAGAACGAACGTGTTTGCGGAAACATGTTTCGCTGCAAGCTCACAGGGCTGACACACATCTGTGATAAAAACTGTAACCAGAGAATTCTGTATGATAACCATAGCTCCCTTTGCCTAGCAAGTCGTCAGATTTTCCCCCTGACTCCAACAGAAGAACAGGCAGTGAGAGGCGTTCGAAGGAAGCTCGACGCGGCAGAGAGTTCGCCTGTTGATAACATCGGTTGTAAGCGTAGGCGGGATGCACAGTTCCATCCTTCTCCATTTGAGAGATCTTTCTCTGCTGTCAGTCCTATCTGCAGCCAAGTTGGAGACGGCATGGATACAAATTAGATATCGAGATTCATATAACTGAAGGAGATCTTTATCGTTTTTCCGTTTTTTTTTACTTCATTACTGATGAGTATCCATAACTGAACTTGAACACTTATGAATCGCCGATTGGTGGACATGATGTGTCCCCATTCGCCGTAGGTCGCCTTTATGTTTCTAGTAATAATGTAATGCAGTATTTTAAGTAGCTTGACTGTTGAATGGAGATTTGAGCAGGCTGTGTGTAAACAGTTATGAGACTTGAATTGAAATCCGTTTCTTGCTAACTTACCGAGAATCTGACTCTTATAGCTTACCACAGGATATGTTCCGGCATTCTATGATGCTTTAATTTATATGGACCTGGTTGATGTAATTATTAGCAATGTTTCTCATTTTCATGTATCTGTAATCATGAAGCGTCCATATTCTAGTATTAGAGTGGCACACTTGTGTATTCTTCCACTTTTGTTTACTATGGTTCTGTTAAAACAGAAAAAAGTAATCGATCTTGTTCCTGCTGGTAGCTCAGGTTTTATCCAGTTCTTCATATGCAAGTTTTTGGATTCTTATGACGGCTGGTTTACTAACTTTTCCTCTGTTACAGGCTCAAGGGAGATGATACTAGTTTTCATTCAAACTTGTTTTGAATTGAATTTCTGTTATTTTTTATGGTTAAAATTGATTGACAAATGGCTGTGTGAGTTGAATGTAAATTGTGCAGGTTTTGAAGCATATGTGCTAGGAAGGTGCCTTCAAAATATGTGTTACAATATTGAATGATTTCTTATGATCGATTTTCTCTGTCATAATGACATGAAATACTGATAAAATTTCATTTTCTTGATGTAGGTACATCCATTGCAAGTTTAGCTTGTGTCTCTCTGTCTTGGCCAAAGTTTGGAGAATGGAGCTGTGAAGAGTTAAAAGGATATTTTGACGCTACAAGATGTCAAAAGAATTGAGGCAGGAGCAGTTATGACACTATATCGAAGGCTGATAAAAATGTGAGGTGAAATTGAGCGAATAAAGGGTTGTCCTCACCAAGAAATCTATCATCACTGTCACCAAAAATATCTGCATAATGTGTTCAAGGATTGAAAGTAGAGTTTCCTTTGAGGATGTGCAGATGGGCTAGATAGCATGACCTCAAATTTATCATAGGGTCTCATAATAGATTTGTTAGAGTTAAACCATGATTAAATAGGGTTGATAATCATGGTTAACCTATACAGGATCTTCAAAAATTTCAGAAAACCCTGATTGAAAGTAATAGCTTGTTTTTGAATTGAAGGATTGAATGTAACTTATCTGAATTGCCTTGTTCATGGCATCAGCTGAATTTCTTACTTCTGTTTCATTCTTAGCATATTTGTGAAGCTAGAAGATCGTTCTATTTTTTTCCTCCAATTTTGATGTCTCTTATTTCATACCTTGTTTCTTGTAAGTATCCAATCAATGAAATTCTTTTCAATAAAAAGT
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9992
PRIMER_LEFT_PENALTY=1.629294
PRIMER_RIGHT_PENALTY=0.369874
PRIMER_LEFT_SEQUENCE=TTTGTTAGTTCGGTTTAGGTGC
PRIMER_RIGHT_SEQUENCE=CTTCCCTGAATCGTATGCTTTT
PRIMER_LEFT=13,22
PRIMER_RIGHT=187,22
PRIMER_LEFT_TM=58.371
PRIMER_RIGHT_TM=59.630
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=175
PRIMER_PAIR_PENALTY_1=2.0851
PRIMER_LEFT_1_PENALTY=1.629294
PRIMER_RIGHT_1_PENALTY=0.455824
PRIMER_LEFT_1_SEQUENCE=TTTGTTAGTTCGGTTTAGGTGC
PRIMER_RIGHT_1_SEQUENCE=AATTTCACTCCAACTTTACGGC
PRIMER_LEFT_1=13,22
PRIMER_RIGHT_1=209,22
PRIMER_LEFT_1_TM=58.371
PRIMER_RIGHT_1_TM=59.544
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=11.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=2.7107
PRIMER_LEFT_2_PENALTY=1.629294
PRIMER_RIGHT_2_PENALTY=1.081398
PRIMER_LEFT_2_SEQUENCE=TTTGTTAGTTCGGTTTAGGTGC
PRIMER_RIGHT_2_SEQUENCE=CCCTGAATCGTATGCTTTTGA
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=184,21
PRIMER_LEFT_2_TM=58.371
PRIMER_RIGHT_2_TM=60.081
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=2.7107
PRIMER_LEFT_3_PENALTY=1.629294
PRIMER_RIGHT_3_PENALTY=1.081398
PRIMER_LEFT_3_SEQUENCE=TTTGTTAGTTCGGTTTAGGTGC
PRIMER_RIGHT_3_SEQUENCE=TCCCTGAATCGTATGCTTTTG
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=185,21
PRIMER_LEFT_3_TM=58.371
PRIMER_RIGHT_3_TM=60.081
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=173
PRIMER_PAIR_PENALTY_4=2.8636
PRIMER_LEFT_4_PENALTY=1.629294
PRIMER_RIGHT_4_PENALTY=1.234350
PRIMER_LEFT_4_SEQUENCE=TTTGTTAGTTCGGTTTAGGTGC
PRIMER_RIGHT_4_SEQUENCE=CCTGAATCGTATGCTTTTGAAC
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=183,22
PRIMER_LEFT_4_TM=58.371
PRIMER_RIGHT_4_TM=58.766
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_03391 Ps_contig_mira-and-tgicl-ass_franssen_29854
SEQUENCE=CCTTAACAGAAACACATTAAAAAACAAAGARRACTAATATACAGCAAACAAATATAATAAATACATCAAACATTTCAACTTGCAAGATTAAAGCCAAAGTCATTCATACATACATAATACTAAAGGGATTGACAATTGATACAAAACAAATCCAATCCTCAAAACCCTAAGATAGATGTCCAAAAGCCACATAGTAGAGAGTTAATTAATCAAAGATGAAAAGCACTTGGTTGTGATTTTGTCATCCTTGCTTCACCATAAGACTCATCATTATCCATCCTGCGGTTGTTTCTATAAGCATAGTAAGCAACTATGTAAACAATTACAAGGATGATCATAACAATGATGTTGATCACTGATACTTTCCTCCAACTTTTCTTGAGACTAGCAAGTACACCAGCTTTGCAAGAATCACAGCTATAACAAAGCTCTTGTTGGTCATTGTTCCATTTAGTGCAGTCAGGGTTGTTTGCTATTAGTCCTACTCCTGGCATCCACACTGTTTCATTTTGATACATGAAGCCACAATCTGTTGGAGGTTTGCAGCAACCAGACTGAACAGAAGAAAGTTTTCTCTGGAAGAACATATCAGCAGTTTCCGGCTGACCGTTAAAATTCCTGGCTAATTTACGACAAACTTTAGAATCCCTTATACAAGAACTAATCTTCCCCCAGTAACCATCCCTGGCAACACGTTCCTCTAACCACCCAGAATAATCTTCCAGGTAATAATCCAAATAGCCTTTATTCGAAAGGCCTCTTCCGGAGCCTTTATCGGTTACAACATAGGCAAAGATGATGAAGCCTATAAGAACAGCGATGACGCAAAACATGACGACGAGGTAGAGCCGTAGGAGAAAGGTGTTTCTGTAACACGCGCCGGCGAAACCGGCTAGTGAAACAACCATGATGCTTATGCCGATTATGATTAGTGGCCATTGGAGGAATTTGAGACAGTCGGTGTTGTTTGCTCGGCTGCTTAGCCATATTCCACCGCCTAGGATTGGGATTGAGAGAAGGAAGGTTAGGAAGTTTAGTACACCGATTAAGTGGTTGCTTGTTCTCTATTTTTCTTTTTTTTTTGGATTGTGTAATTTTTTTGGTTTGATTTGCTTAGTAGG
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03392 Ps_contig_mira-and-tgicl-ass_franssen_29858
SEQUENCE=CGTGCTCAGAGGGAAGTAATGACCGACATGGATATAGAAAGAAAACAAGTACTCAGGTGTCGAGTATGGATTATGGAANAGAATTATACATTCCATGTACTGGTGCCCTAAATTGAAACTTGTCTCCAGAGGTTCCCTTGATATCTTGGACAACATTACCACCAGGAGAAGTTACCTTGAAATCGATGCCAGGGTGATCGGAGCTCCAGAAAATAATCATGGTCAACAACAACAAAATTCCCCGAAACAGTGGTCCCCTCTGTAAAGCACATACTCATAGAGACATTCAACTTCATTCACTGTCCAACAAGAAAAGAGATTCAATCCGATCAACGAANATAAAACATACAAAACACATACCAAAAACCNTCCAACA
TARGET=224,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2904
PRIMER_LEFT_PENALTY=0.108684
PRIMER_RIGHT_PENALTY=0.181752
PRIMER_LEFT_SEQUENCE=TCTTGGACAACATTACCACCAG
PRIMER_RIGHT_SEQUENCE=GTTGGANGGTTTTTGGTATGTG
PRIMER_LEFT=143,22
PRIMER_RIGHT=374,22
PRIMER_LEFT_TM=59.891
PRIMER_RIGHT_TM=59.818
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.4540
PRIMER_LEFT_1_PENALTY=0.081468
PRIMER_RIGHT_1_PENALTY=0.372557
PRIMER_LEFT_1_SEQUENCE=GGAAGTAATGACCGACATGGAT
PRIMER_RIGHT_1_SEQUENCE=TATGTGCTTTACAGAGGGGACC
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=60.373
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.4761
PRIMER_LEFT_2_PENALTY=0.081468
PRIMER_RIGHT_2_PENALTY=0.394672
PRIMER_LEFT_2_SEQUENCE=GGAAGTAATGACCGACATGGAT
PRIMER_RIGHT_2_SEQUENCE=TTACAGAGGGGACCACTGTTTC
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=264,22
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.395
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=0.4812
PRIMER_LEFT_3_PENALTY=0.108684
PRIMER_RIGHT_3_PENALTY=0.372557
PRIMER_LEFT_3_SEQUENCE=TCTTGGACAACATTACCACCAG
PRIMER_RIGHT_3_SEQUENCE=TATGTGCTTTACAGAGGGGACC
PRIMER_LEFT_3=143,22
PRIMER_RIGHT_3=272,22
PRIMER_LEFT_3_TM=59.891
PRIMER_RIGHT_3_TM=60.373
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=130
PRIMER_PAIR_PENALTY_4=0.5034
PRIMER_LEFT_4_PENALTY=0.108684
PRIMER_RIGHT_4_PENALTY=0.394672
PRIMER_LEFT_4_SEQUENCE=TCTTGGACAACATTACCACCAG
PRIMER_RIGHT_4_SEQUENCE=TTACAGAGGGGACCACTGTTTC
PRIMER_LEFT_4=143,22
PRIMER_RIGHT_4=264,22
PRIMER_LEFT_4_TM=59.891
PRIMER_RIGHT_4_TM=60.395
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=122
=
PRIMER_SEQUENCE_ID=SSR_03393 Ps_contig_mira-and-tgicl-ass_franssen_29859
SEQUENCE=CTTNAAGACCACGACGACTACAGTATTGCCGTTTGGTTGCGTTGCACTTGCAGTGACGAGAAAGGTTCAAAACCTTCTCAAAATCCAATCTTTAAGATCTGCAATACACAATTATCGATTTTTTTTCAATTCCAGAAAAAACCCCCAAATTAAATAAATAAAAAGGGGGAGAAGTGATGGAGCGAAACCAGGGAAAGAGAAGCGCTAAGATGTGGATGGTTTTGATGTGTTTGATGTTTATCTTCGTTGATCGGATTGAATCTCTTTCTGTGACAGTGAATGAAGTTGAATGTCTCTATGAGTACGTGCTTTACGAGGGGGACACTGTTTCGGGGAATTTTGTTGTTGTTGACCATGATATTTTCTGGAGCTCCGATCACCCTGGCATCGATTTCAAGGTAACTTCTCCTGGTGGTAACGTTGTCCAAGATATCAAGGGAACCTCTGGAGACAAGTTTCAATTTAAGGCACCAGTACATGGAATGTATAAATTCTGTTTCCATAATCCATATTCGACCCCCGAGACTGTTTCTTTCTACATCCATGTCGGTCATATTCCCTCTGAGCACGATCTTGCCAAAGATGAGCATTTGGACCCAATTAATGTTAAAATTGCCGAGCTGAGAGAGGCATTGGACTCTGTTACAGCTGAACAGAAGTACTTGAGAGCTCGTGATGCAAGACATCGCCACACAAATGAGAGTACACATAAGCGCGTAATCTTCTACACTGTGGGAGAGTATCTTCTGTTGGCTGCTGTTAGTGCACTGCAGGTCGTATACATCAGGCGCCTTTTCAGCAAATCTGTGGCTTACAACCGTGTTTGAGTCCGTCCCTCATTAATTAATCTTATTATGCTTTCTATCTTTTAGTTAAATTAAATTAAAGGTTAGGGGTTTATAGACATATTTTCTCTGTGAAACATTATAATACTATTCTTGGTGGTGAACATTTTGTTTCTCTTTTCGACACCGAGGTCACTTGTACTTTCCATACAACAAGGTTTTTGTATTCCAACGTTTTTAAATCGTGTGAGATTAATGTTATGCAAAATGATACTCTTGTGCTTTTATGTTAAATTTAGAAAAAAAAAA
TARGET=151,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3849
PRIMER_LEFT_PENALTY=0.343387
PRIMER_RIGHT_PENALTY=0.041553
PRIMER_LEFT_SEQUENCE=ACTTGCAGTGACGAGAAAGGTT
PRIMER_RIGHT_SEQUENCE=CCACATCTTAGCGCTTCTCTTT
PRIMER_LEFT=45,22
PRIMER_RIGHT=214,22
PRIMER_LEFT_TM=60.343
PRIMER_RIGHT_TM=60.042
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.4362
PRIMER_LEFT_1_PENALTY=0.425301
PRIMER_RIGHT_1_PENALTY=0.010890
PRIMER_LEFT_1_SEQUENCE=CTACAGTATTGCCGTTTGGTTG
PRIMER_RIGHT_1_SEQUENCE=ATCTTAGCGCTTCTCTTTCCCT
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=210,22
PRIMER_LEFT_1_TM=59.575
PRIMER_RIGHT_1_TM=60.011
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=194
PRIMER_PAIR_PENALTY_2=0.4669
PRIMER_LEFT_2_PENALTY=0.425301
PRIMER_RIGHT_2_PENALTY=0.041553
PRIMER_LEFT_2_SEQUENCE=CTACAGTATTGCCGTTTGGTTG
PRIMER_RIGHT_2_SEQUENCE=CCACATCTTAGCGCTTCTCTTT
PRIMER_LEFT_2=17,22
PRIMER_RIGHT_2=214,22
PRIMER_LEFT_2_TM=59.575
PRIMER_RIGHT_2_TM=60.042
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=0.5576
PRIMER_LEFT_3_PENALTY=0.425301
PRIMER_RIGHT_3_PENALTY=0.132262
PRIMER_LEFT_3_SEQUENCE=CTACAGTATTGCCGTTTGGTTG
PRIMER_RIGHT_3_SEQUENCE=CAAACACATCAAAACCATCCAC
PRIMER_LEFT_3=17,22
PRIMER_RIGHT_3=232,22
PRIMER_LEFT_3_TM=59.575
PRIMER_RIGHT_3_TM=60.132
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=216
PRIMER_PAIR_PENALTY_4=0.6448
PRIMER_LEFT_4_PENALTY=0.603210
PRIMER_RIGHT_4_PENALTY=0.041553
PRIMER_LEFT_4_SEQUENCE=CAGTGACGAGAAAGGTTCAAAA
PRIMER_RIGHT_4_SEQUENCE=CCACATCTTAGCGCTTCTCTTT
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=214,22
PRIMER_LEFT_4_TM=59.397
PRIMER_RIGHT_4_TM=60.042
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_03394 Ps_contig_mira-and-tgicl-ass_franssen_29859
SEQUENCE=CTTNAAGACCACGACGACTACAGTATTGCCGTTTGGTTGCGTTGCACTTGCAGTGACGAGAAAGGTTCAAAACCTTCTCAAAATCCAATCTTTAAGATCTGCAATACACAATTATCGATTTTTTTTCAATTCCAGAAAAAACCCCCAAATTAAATAAATAAAAAGGGGGAGAAGTGATGGAGCGAAACCAGGGAAAGAGAAGCGCTAAGATGTGGATGGTTTTGATGTGTTTGATGTTTATCTTCGTTGATCGGATTGAATCTCTTTCTGTGACAGTGAATGAAGTTGAATGTCTCTATGAGTACGTGCTTTACGAGGGGGACACTGTTTCGGGGAATTTTGTTGTTGTTGACCATGATATTTTCTGGAGCTCCGATCACCCTGGCATCGATTTCAAGGTAACTTCTCCTGGTGGTAACGTTGTCCAAGATATCAAGGGAACCTCTGGAGACAAGTTTCAATTTAAGGCACCAGTACATGGAATGTATAAATTCTGTTTCCATAATCCATATTCGACCCCCGAGACTGTTTCTTTCTACATCCATGTCGGTCATATTCCCTCTGAGCACGATCTTGCCAAAGATGAGCATTTGGACCCAATTAATGTTAAAATTGCCGAGCTGAGAGAGGCATTGGACTCTGTTACAGCTGAACAGAAGTACTTGAGAGCTCGTGATGCAAGACATCGCCACACAAATGAGAGTACACATAAGCGCGTAATCTTCTACACTGTGGGAGAGTATCTTCTGTTGGCTGCTGTTAGTGCACTGCAGGTCGTATACATCAGGCGCCTTTTCAGCAAATCTGTGGCTTACAACCGTGTTTGAGTCCGTCCCTCATTAATTAATCTTATTATGCTTTCTATCTTTTAGTTAAATTAAATTAAAGGTTAGGGGTTTATAGACATATTTTCTCTGTGAAACATTATAATACTATTCTTGGTGGTGAACATTTTGTTTCTCTTTTCGACACCGAGGTCACTTGTACTTTCCATACAACAAGGTTTTTGTATTCCAACGTTTTTAAATCGTGTGAGATTAATGTTATGCAAAATGATACTCTTGTGCTTTTATGTTAAATTTAGAAAAAAAAAA
TARGET=340,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0721
PRIMER_LEFT_PENALTY=0.010890
PRIMER_RIGHT_PENALTY=0.061230
PRIMER_LEFT_SEQUENCE=AGGGAAAGAGAAGCGCTAAGAT
PRIMER_RIGHT_SEQUENCE=ATCGGAGCTCCAGAAAATATCA
PRIMER_LEFT=189,22
PRIMER_RIGHT=376,22
PRIMER_LEFT_TM=60.011
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.0721
PRIMER_LEFT_1_PENALTY=0.010890
PRIMER_RIGHT_1_PENALTY=0.061230
PRIMER_LEFT_1_SEQUENCE=AGGGAAAGAGAAGCGCTAAGAT
PRIMER_RIGHT_1_SEQUENCE=TCGGAGCTCCAGAAAATATCAT
PRIMER_LEFT_1=189,22
PRIMER_RIGHT_1=375,22
PRIMER_LEFT_1_TM=60.011
PRIMER_RIGHT_1_TM=60.061
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.1028
PRIMER_LEFT_2_PENALTY=0.041553
PRIMER_RIGHT_2_PENALTY=0.061230
PRIMER_LEFT_2_SEQUENCE=AAAGAGAAGCGCTAAGATGTGG
PRIMER_RIGHT_2_SEQUENCE=ATCGGAGCTCCAGAAAATATCA
PRIMER_LEFT_2=193,22
PRIMER_RIGHT_2=376,22
PRIMER_LEFT_2_TM=60.042
PRIMER_RIGHT_2_TM=60.061
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=0.1028
PRIMER_LEFT_3_PENALTY=0.041553
PRIMER_RIGHT_3_PENALTY=0.061230
PRIMER_LEFT_3_SEQUENCE=AAAGAGAAGCGCTAAGATGTGG
PRIMER_RIGHT_3_SEQUENCE=TCGGAGCTCCAGAAAATATCAT
PRIMER_LEFT_3=193,22
PRIMER_RIGHT_3=375,22
PRIMER_LEFT_3_TM=60.042
PRIMER_RIGHT_3_TM=60.061
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.1282
PRIMER_LEFT_4_PENALTY=0.010890
PRIMER_RIGHT_4_PENALTY=0.117281
PRIMER_LEFT_4_SEQUENCE=AGGGAAAGAGAAGCGCTAAGAT
PRIMER_RIGHT_4_SEQUENCE=CATTCCATGTACTGGTGCCTTA
PRIMER_LEFT_4=189,22
PRIMER_RIGHT_4=484,22
PRIMER_LEFT_4_TM=60.011
PRIMER_RIGHT_4_TM=59.883
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_03395 Ps_contig_mira-and-tgicl-ass_franssen_29859
SEQUENCE=CTTNAAGACCACGACGACTACAGTATTGCCGTTTGGTTGCGTTGCACTTGCAGTGACGAGAAAGGTTCAAAACCTTCTCAAAATCCAATCTTTAAGATCTGCAATACACAATTATCGATTTTTTTTCAATTCCAGAAAAAACCCCCAAATTAAATAAATAAAAAGGGGGAGAAGTGATGGAGCGAAACCAGGGAAAGAGAAGCGCTAAGATGTGGATGGTTTTGATGTGTTTGATGTTTATCTTCGTTGATCGGATTGAATCTCTTTCTGTGACAGTGAATGAAGTTGAATGTCTCTATGAGTACGTGCTTTACGAGGGGGACACTGTTTCGGGGAATTTTGTTGTTGTTGACCATGATATTTTCTGGAGCTCCGATCACCCTGGCATCGATTTCAAGGTAACTTCTCCTGGTGGTAACGTTGTCCAAGATATCAAGGGAACCTCTGGAGACAAGTTTCAATTTAAGGCACCAGTACATGGAATGTATAAATTCTGTTTCCATAATCCATATTCGACCCCCGAGACTGTTTCTTTCTACATCCATGTCGGTCATATTCCCTCTGAGCACGATCTTGCCAAAGATGAGCATTTGGACCCAATTAATGTTAAAATTGCCGAGCTGAGAGAGGCATTGGACTCTGTTACAGCTGAACAGAAGTACTTGAGAGCTCGTGATGCAAGACATCGCCACACAAATGAGAGTACACATAAGCGCGTAATCTTCTACACTGTGGGAGAGTATCTTCTGTTGGCTGCTGTTAGTGCACTGCAGGTCGTATACATCAGGCGCCTTTTCAGCAAATCTGTGGCTTACAACCGTGTTTGAGTCCGTCCCTCATTAATTAATCTTATTATGCTTTCTATCTTTTAGTTAAATTAAATTAAAGGTTAGGGGTTTATAGACATATTTTCTCTGTGAAACATTATAATACTATTCTTGGTGGTGAACATTTTGTTTCTCTTTTCGACACCGAGGTCACTTGTACTTTCCATACAACAAGGTTTTTGTATTCCAACGTTTTTAAATCGTGTGAGATTAATGTTATGCAAAATGATACTCTTGTGCTTTTATGTTAAATTTAGAAAAAAAAAA
TARGET=873,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3410
PRIMER_LEFT_PENALTY=0.110004
PRIMER_RIGHT_PENALTY=1.230989
PRIMER_LEFT_SEQUENCE=CTTCTGTTGGCTGCTGTTAGTG
PRIMER_RIGHT_SEQUENCE=GAAAGTACAAGTGACCTCGGTG
PRIMER_LEFT=743,22
PRIMER_RIGHT=990,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=58.769
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=1.3575
PRIMER_LEFT_1_PENALTY=0.088435
PRIMER_RIGHT_1_PENALTY=1.269097
PRIMER_LEFT_1_SEQUENCE=GTGCACTGCAGGTCGTATACAT
PRIMER_RIGHT_1_SEQUENCE=CGGTGTCGAAAAGAGAAACAA
PRIMER_LEFT_1=762,22
PRIMER_RIGHT_1=973,21
PRIMER_LEFT_1_TM=60.088
PRIMER_RIGHT_1_TM=60.269
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=1.3791
PRIMER_LEFT_2_PENALTY=0.110004
PRIMER_RIGHT_2_PENALTY=1.269097
PRIMER_LEFT_2_SEQUENCE=CTTCTGTTGGCTGCTGTTAGTG
PRIMER_RIGHT_2_SEQUENCE=CGGTGTCGAAAAGAGAAACAA
PRIMER_LEFT_2=743,22
PRIMER_RIGHT_2=973,21
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.269
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=1.4183
PRIMER_LEFT_3_PENALTY=0.187326
PRIMER_RIGHT_3_PENALTY=1.230989
PRIMER_LEFT_3_SEQUENCE=CAGCAAATCTGTGGCTTACAAC
PRIMER_RIGHT_3_SEQUENCE=GAAAGTACAAGTGACCTCGGTG
PRIMER_LEFT_3=796,22
PRIMER_RIGHT_3=990,22
PRIMER_LEFT_3_TM=59.813
PRIMER_RIGHT_3_TM=58.769
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=1.4564
PRIMER_LEFT_4_PENALTY=0.187326
PRIMER_RIGHT_4_PENALTY=1.269097
PRIMER_LEFT_4_SEQUENCE=CAGCAAATCTGTGGCTTACAAC
PRIMER_RIGHT_4_SEQUENCE=CGGTGTCGAAAAGAGAAACAA
PRIMER_LEFT_4=796,22
PRIMER_RIGHT_4=973,21
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=60.269
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_03396 Ps_contig_mira-and-tgicl-ass_franssen_29860
SEQUENCE=AGTCATAGCGAAGCTATGCTTCAAGACCACGACGACTACAGTATTGCCGTTTTGTTGCGTTGCACTTGCAGTGACGAGAAAGGTTCAAAACCTTCTCAAAATCCAATCTTTAAGATCTGCAATACACAATTATCGATTTTTTTTCAATTCCAGAAAAAAACCCCAAATTAAATAAATAAAAAGGGGGAGAAGTGATGGAGCGAAATCAGGGAAAGAGAAGCGCTAAGATGTGGATGGTTTTGATGTGTTTGATGTTTATCTTCGTTGATCGGATTGAATCTCTTTCTGTGACAGTGAATGAAGTTGAATGTCTCTATGAGTATGTGCTTTACGAGGGGGACACTGTTTCGGGGAATTTTGTTGTTGTTGACCATGATATTTTCTGGAGCTCCGATCACCCTGGCATCGATTTCAAGGTAACTTCTCCTGGTGGTAATGTTGTCCAAGATATCAAGGGAACCTCTGGAGACAAGTTTCAATTTAAGGCACCAGTACATGGAATGTATAAATTCTGTTTCCATAATCCATACTCGACACCTGAGACTGTTTCTTTCTATATCCATGTCGGTCATATTCCCTCTGAGCACGATCTTGCCAAAGATGAGCATTTGGACCCAATTAATGTTAAAATTGCCGAGCTGAGAGAGGCATTGGACTCTGTTACAGCTGAACAAAAGTACTTGAGAGCTCGTGATGCAAGACATCGCCACACAAATGAGAGTACACATAAGCGCGTAATCTTCTACACTGTGGGAGAGTATCTTCTGTTGGCTGCTGTTAGTGCACTGCAGGTCGTATACATCAGGCGCCTTTTCAGCAAATCTGTGGCTTACAACCGTGTTTGAGTCCGTCCCTCATTAATTAATCTTATTATGCTTTCTATTCTTTTNRAGTTTAAAATTTAAAATTTAAAAGGGTTTAAGGGGTTTTAATAGACATTATTT
TARGET=169,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03397 Ps_contig_mira-and-tgicl-ass_franssen_29860
SEQUENCE=AGTCATAGCGAAGCTATGCTTCAAGACCACGACGACTACAGTATTGCCGTTTTGTTGCGTTGCACTTGCAGTGACGAGAAAGGTTCAAAACCTTCTCAAAATCCAATCTTTAAGATCTGCAATACACAATTATCGATTTTTTTTCAATTCCAGAAAAAAACCCCAAATTAAATAAATAAAAAGGGGGAGAAGTGATGGAGCGAAATCAGGGAAAGAGAAGCGCTAAGATGTGGATGGTTTTGATGTGTTTGATGTTTATCTTCGTTGATCGGATTGAATCTCTTTCTGTGACAGTGAATGAAGTTGAATGTCTCTATGAGTATGTGCTTTACGAGGGGGACACTGTTTCGGGGAATTTTGTTGTTGTTGACCATGATATTTTCTGGAGCTCCGATCACCCTGGCATCGATTTCAAGGTAACTTCTCCTGGTGGTAATGTTGTCCAAGATATCAAGGGAACCTCTGGAGACAAGTTTCAATTTAAGGCACCAGTACATGGAATGTATAAATTCTGTTTCCATAATCCATACTCGACACCTGAGACTGTTTCTTTCTATATCCATGTCGGTCATATTCCCTCTGAGCACGATCTTGCCAAAGATGAGCATTTGGACCCAATTAATGTTAAAATTGCCGAGCTGAGAGAGGCATTGGACTCTGTTACAGCTGAACAAAAGTACTTGAGAGCTCGTGATGCAAGACATCGCCACACAAATGAGAGTACACATAAGCGCGTAATCTTCTACACTGTGGGAGAGTATCTTCTGTTGGCTGCTGTTAGTGCACTGCAGGTCGTATACATCAGGCGCCTTTTCAGCAAATCTGTGGCTTACAACCGTGTTTGAGTCCGTCCCTCATTAATTAATCTTATTATGCTTTCTATTCTTTTNRAGTTTAAAATTTAAAATTTAAAAGGGTTTAAGGGGTTTTAATAGACATTATTT
TARGET=358,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03398 Ps_contig_mira-and-tgicl-ass_franssen_29865
SEQUENCE=CCGAAAARATATAATAAAAAAAAAGAATAACTTTTTTCCATCATCACTCAACAACTTTCACACGAAACTTGAAAGACATGAATGACGCCCAGAAAACTATTCATCATCCTCCACAAAAGATAAACTGAATCACTTAGTGATAATGTAAATAGCATAGAGAATTCCAGGAAGATAGCCAAAAAGAGTGAGCACCAAACAGATCCAAAACTCCACTTCACAGCCAAACTTGAGGAAGACACCAAGAGGTGGGAGGAGGATGGCAAGAATGATATCAATGAAGGTAGCTGTACTCATTTTGTTTGTTTGAGAGAGAGAGAGGGAGAGAGAGAGTGTGAAGAAGAAGAAGACTTTAAAGTTGAGGACAAGACAATGAATGTTATGTTATGTTATCGCCCCNGCGTA
TARGET=295,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03399 Ps_contig_mira-and-tgicl-ass_franssen_29865
SEQUENCE=CCGAAAARATATAATAAAAAAAAAGAATAACTTTTTTCCATCATCACTCAACAACTTTCACACGAAACTTGAAAGACATGAATGACGCCCAGAAAACTATTCATCATCCTCCACAAAAGATAAACTGAATCACTTAGTGATAATGTAAATAGCATAGAGAATTCCAGGAAGATAGCCAAAAAGAGTGAGCACCAAACAGATCCAAAACTCCACTTCACAGCCAAACTTGAGGAAGACACCAAGAGGTGGGAGGAGGATGGCAAGAATGATATCAATGAAGGTAGCTGTACTCATTTTGTTTGTTTGAGAGAGAGAGAGGGAGAGAGAGAGTGTGAAGAAGAAGAAGACTTTAAAGTTGAGGACAAGACAATGAATGTTATGTTATGTTATCGCCCCNGCGTA
TARGET=306,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03400 Ps_contig_mira-and-tgicl-ass_franssen_29865
SEQUENCE=CCGAAAARATATAATAAAAAAAAAGAATAACTTTTTTCCATCATCACTCAACAACTTTCACACGAAACTTGAAAGACATGAATGACGCCCAGAAAACTATTCATCATCCTCCACAAAAGATAAACTGAATCACTTAGTGATAATGTAAATAGCATAGAGAATTCCAGGAAGATAGCCAAAAAGAGTGAGCACCAAACAGATCCAAAACTCCACTTCACAGCCAAACTTGAGGAAGACACCAAGAGGTGGGAGGAGGATGGCAAGAATGATATCAATGAAGGTAGCTGTACTCATTTTGTTTGTTTGAGAGAGAGAGAGGGAGAGAGAGAGTGTGAAGAAGAAGAAGACTTTAAAGTTGAGGACAAGACAATGAATGTTATGTTATGTTATCGCCCCNGCGTA
TARGET=307,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03401 Ps_contig_mira-and-tgicl-ass_franssen_29865
SEQUENCE=CCGAAAARATATAATAAAAAAAAAGAATAACTTTTTTCCATCATCACTCAACAACTTTCACACGAAACTTGAAAGACATGAATGACGCCCAGAAAACTATTCATCATCCTCCACAAAAGATAAACTGAATCACTTAGTGATAATGTAAATAGCATAGAGAATTCCAGGAAGATAGCCAAAAAGAGTGAGCACCAAACAGATCCAAAACTCCACTTCACAGCCAAACTTGAGGAAGACACCAAGAGGTGGGAGGAGGATGGCAAGAATGATATCAATGAAGGTAGCTGTACTCATTTTGTTTGTTTGAGAGAGAGAGAGGGAGAGAGAGAGTGTGAAGAAGAAGAAGACTTTAAAGTTGAGGACAAGACAATGAATGTTATGTTATGTTATCGCCCCNGCGTA
TARGET=320,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03402 Ps_contig_mira-and-tgicl-ass_franssen_29865
SEQUENCE=CCGAAAARATATAATAAAAAAAAAGAATAACTTTTTTCCATCATCACTCAACAACTTTCACACGAAACTTGAAAGACATGAATGACGCCCAGAAAACTATTCATCATCCTCCACAAAAGATAAACTGAATCACTTAGTGATAATGTAAATAGCATAGAGAATTCCAGGAAGATAGCCAAAAAGAGTGAGCACCAAACAGATCCAAAACTCCACTTCACAGCCAAACTTGAGGAAGACACCAAGAGGTGGGAGGAGGATGGCAAGAATGATATCAATGAAGGTAGCTGTACTCATTTTGTTTGTTTGAGAGAGAGAGAGGGAGAGAGAGAGTGTGAAGAAGAAGAAGACTTTAAAGTTGAGGACAAGACAATGAATGTTATGTTATGTTATCGCCCCNGCGTA
TARGET=334,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03403 Ps_contig_mira-and-tgicl-ass_franssen_29865
SEQUENCE=CCGAAAARATATAATAAAAAAAAAGAATAACTTTTTTCCATCATCACTCAACAACTTTCACACGAAACTTGAAAGACATGAATGACGCCCAGAAAACTATTCATCATCCTCCACAAAAGATAAACTGAATCACTTAGTGATAATGTAAATAGCATAGAGAATTCCAGGAAGATAGCCAAAAAGAGTGAGCACCAAACAGATCCAAAACTCCACTTCACAGCCAAACTTGAGGAAGACACCAAGAGGTGGGAGGAGGATGGCAAGAATGATATCAATGAAGGTAGCTGTACTCATTTTGTTTGTTTGAGAGAGAGAGAGGGAGAGAGAGAGTGTGAAGAAGAAGAAGACTTTAAAGTTGAGGACAAGACAATGAATGTTATGTTATGTTATCGCCCCNGCGTA
TARGET=374,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03404 Ps_contig_mira-and-tgicl-ass_franssen_29866
SEQUENCE=GGGGATCGCATAACATAACATAACATTCATTGTCTTGTCCTCAACTTTAAAGTCTTCTTCTTCATCACACACTCTCTCTCTCTCTCAAACAAACAAAATGAGTACAGCTACCTTCATTGATATCATTCTTGCCATCCTCCTCCCACCTCTTGGTGTCTTCCTCAAGTTTGGCTGTGAAGTGGAGTTTTGGATCTGTTTGGTGCTTACTTCTTTTTGGCTATCTTCCTGGAATTCTCTATGCTATTTACATTATCACTAAGTGATTCAGTTTATCTTTTGTGGAGGATGATGAATAGTTTTCTGGGCGTCATTCATGTCTTTCAAGTTTCGTGTGAAAGTTGTTGAGTGATGATAGGAAAAAAGTTATTT
TARGET=9,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03405 Ps_contig_mira-and-tgicl-ass_franssen_29866
SEQUENCE=GGGGATCGCATAACATAACATAACATTCATTGTCTTGTCCTCAACTTTAAAGTCTTCTTCTTCATCACACACTCTCTCTCTCTCTCAAACAAACAAAATGAGTACAGCTACCTTCATTGATATCATTCTTGCCATCCTCCTCCCACCTCTTGGTGTCTTCCTCAAGTTTGGCTGTGAAGTGGAGTTTTGGATCTGTTTGGTGCTTACTTCTTTTTGGCTATCTTCCTGGAATTCTCTATGCTATTTACATTATCACTAAGTGATTCAGTTTATCTTTTGTGGAGGATGATGAATAGTTTTCTGGGCGTCATTCATGTCTTTCAAGTTTCGTGTGAAAGTTGTTGAGTGATGATAGGAAAAAAGTTATTT
TARGET=72,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3206
PRIMER_LEFT_PENALTY=1.280684
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=GGGGATCGCATAACATAACATA
PRIMER_RIGHT_SEQUENCE=AGCACCAAACAGATCCAAAACT
PRIMER_LEFT=0,22
PRIMER_RIGHT=203,22
PRIMER_LEFT_TM=58.719
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=1.4775
PRIMER_LEFT_1_PENALTY=1.280684
PRIMER_RIGHT_1_PENALTY=0.196811
PRIMER_LEFT_1_SEQUENCE=GGGGATCGCATAACATAACATA
PRIMER_RIGHT_1_SEQUENCE=TCCAGGAAGATAGCCAAAAAGA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=229,22
PRIMER_LEFT_1_TM=58.719
PRIMER_RIGHT_1_TM=60.197
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=1.4900
PRIMER_LEFT_2_PENALTY=1.280684
PRIMER_RIGHT_2_PENALTY=0.209343
PRIMER_LEFT_2_SEQUENCE=GGGGATCGCATAACATAACATA
PRIMER_RIGHT_2_SEQUENCE=AACTCCACTTCACAGCCAAACT
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=185,22
PRIMER_LEFT_2_TM=58.719
PRIMER_RIGHT_2_TM=60.209
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=1.4900
PRIMER_LEFT_3_PENALTY=1.280684
PRIMER_RIGHT_3_PENALTY=0.209343
PRIMER_LEFT_3_SEQUENCE=GGGGATCGCATAACATAACATA
PRIMER_RIGHT_3_SEQUENCE=ACTCCACTTCACAGCCAAACTT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=58.719
PRIMER_RIGHT_3_TM=60.209
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=1.5023
PRIMER_LEFT_4_PENALTY=1.280684
PRIMER_RIGHT_4_PENALTY=0.221594
PRIMER_LEFT_4_SEQUENCE=GGGGATCGCATAACATAACATA
PRIMER_RIGHT_4_SEQUENCE=ACTTGAGGAAGACACCAAGAGG
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=166,22
PRIMER_LEFT_4_TM=58.719
PRIMER_RIGHT_4_TM=59.778
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_03406 Ps_contig_mira-and-tgicl-ass_franssen_29866
SEQUENCE=GGGGATCGCATAACATAACATAACATTCATTGTCTTGTCCTCAACTTTAAAGTCTTCTTCTTCATCACACACTCTCTCTCTCTCTCAAACAAACAAAATGAGTACAGCTACCTTCATTGATATCATTCTTGCCATCCTCCTCCCACCTCTTGGTGTCTTCCTCAAGTTTGGCTGTGAAGTGGAGTTTTGGATCTGTTTGGTGCTTACTTCTTTTTGGCTATCTTCCTGGAATTCTCTATGCTATTTACATTATCACTAAGTGATTCAGTTTATCTTTTGTGGAGGATGATGAATAGTTTTCTGGGCGTCATTCATGTCTTTCAAGTTTCGTGTGAAAGTTGTTGAGTGATGATAGGAAAAAAGTTATTT
TARGET=86,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3206
PRIMER_LEFT_PENALTY=1.280684
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=GGGGATCGCATAACATAACATA
PRIMER_RIGHT_SEQUENCE=AGCACCAAACAGATCCAAAACT
PRIMER_LEFT=0,22
PRIMER_RIGHT=203,22
PRIMER_LEFT_TM=58.719
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=1.4775
PRIMER_LEFT_1_PENALTY=1.280684
PRIMER_RIGHT_1_PENALTY=0.196811
PRIMER_LEFT_1_SEQUENCE=GGGGATCGCATAACATAACATA
PRIMER_RIGHT_1_SEQUENCE=TCCAGGAAGATAGCCAAAAAGA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=229,22
PRIMER_LEFT_1_TM=58.719
PRIMER_RIGHT_1_TM=60.197
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=1.4900
PRIMER_LEFT_2_PENALTY=1.280684
PRIMER_RIGHT_2_PENALTY=0.209343
PRIMER_LEFT_2_SEQUENCE=GGGGATCGCATAACATAACATA
PRIMER_RIGHT_2_SEQUENCE=AACTCCACTTCACAGCCAAACT
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=185,22
PRIMER_LEFT_2_TM=58.719
PRIMER_RIGHT_2_TM=60.209
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=1.4900
PRIMER_LEFT_3_PENALTY=1.280684
PRIMER_RIGHT_3_PENALTY=0.209343
PRIMER_LEFT_3_SEQUENCE=GGGGATCGCATAACATAACATA
PRIMER_RIGHT_3_SEQUENCE=ACTCCACTTCACAGCCAAACTT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=58.719
PRIMER_RIGHT_3_TM=60.209
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=1.5023
PRIMER_LEFT_4_PENALTY=1.280684
PRIMER_RIGHT_4_PENALTY=0.221594
PRIMER_LEFT_4_SEQUENCE=GGGGATCGCATAACATAACATA
PRIMER_RIGHT_4_SEQUENCE=ACTTGAGGAAGACACCAAGAGG
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=166,22
PRIMER_LEFT_4_TM=58.719
PRIMER_RIGHT_4_TM=59.778
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_03407 Ps_contig_mira-and-tgicl-ass_franssen_29872
SEQUENCE=CGTGGCGAAGACGTGACAGTAATTGATATTTTTAGCAAGGAAGCAAGCACCCAGAGGCCACAAGGCAGTGACAGATCGACTTTATCAGATTTTTTTGTTGTAACCCCTTATATTTATTTATCCTTCCGTAGATATCTCTCAATGAGAGAGAGAGAAGAACAAGGGTGACTCTCATTCCCCAAACCCTATTCCTCTCTTTCCGTTTCCACGCTTTCTCTTCTCTGACTTTCATTTCAGGATTAACGCCGTCGTTTCGCCGCCGATCATCGTCTGATTAAGTGAAGAATAAACTGATGGCTAACATGGATGGCAAATCACTCCTGAGTCCTAAGAATTCCATTCCCATACCCCCTCCTTCGCCTCAATCAGCAGCAACAGGTTATCCTTGCGAGTATGTGGTGATGAATAATTTAGCACCCAGACGGCGAAGCAGTTGTGTCCCTACTGCAACAAGAGATTTGTGGGAGCGTCTTTTCAATGAAGGTTATAAAGCCGATGTTCGCATTAATACTGATAACAGTGGCATTGTTTATGCTCATTCTAACATTATTGCGGTGGCTTCTCCTGTATTGGGAGGTATGCTGAAGCAAGCAAATCGTTCCAATCGATGGCGATCAATCTCAATTTTTGGGGTTCCACATGATGCAGTACGAGTTTTTATTCGATTCTTGTACTCTTCCTGTTACGAGAACGAAGAAATGAAGGAATTTGTCCTACATCTGTTGGTGCTGTCACATGTATATGCGGTCCCTCACTTGAAGCATGAATGTGAACAGAAGCTAGAATTGGGTTTACTCACCATAGATAATGTAGTGGATGTATTTCAACTTGCATTACTGTGTGATGCACCGCGACTCAGTCTCATTTGTCATCGTAAAATACTAAAAAACTTCAAAACTGTTTCGGAGTCAGAAGGATGGAAAGCAATGAAGCAGAGCCACCCGATTCTAGAAAAGGAACTTCTGGAATCAATGATCGAAGAAGAAAATAGTAAGAAGGAGAGGATCAGAAAAATGAACGAAAAAGAGGTCTATCTGCAATTATACGATGCAATGGAAGCTCTTGTTCACATATGTAGAGATGGTTGTCGAACTATTGGCCCTCATGATAAAGACTTCAAAGCAAACCAACAACCCTGTAGGTATACATCTTGCAAAGGGCTAGAACTGCTTGTTCGCCATTTTGCTGGTTGCAAGTTGAGAGCCCCAGGAGGTTGTGCCCATTGCAAAAGGATGTGGCAACTGTTGGAGCTGCATTCTCGACTCTGTGCTGATCCAAGTTCATGTAGAGTGCCTCTCTGCAGGAACTTTAGACAGAGACTAGCAAAACAAAGCAAGAAAGATGAAATTAGATGGACAATATTGGTTGAAAAGATCTTGAAAACAAGAGGAATTGGGATTGCACCATGCTTTCGGCCACTATGACTGGTTTCCCAGGAATATAGAACTATAGAGTATAGCAGTTGATATATAAAAACTTGTTCAAATTGTAAAGTTTATGAATGTATTATTATGTTGGTATGAAATGTCCCTTAAGGATGAAGTATTATTCCTCTCTATCTTTATTGTAAAAAATTTATCACAGAGGAATCCTTTCTTCCCACCTTGCCTTATTTTCTGATGTGTTAAATAAACTGCATGGCCGAATCAACATTTTAGATTGATTTTGGACCTTTATATGACATTTCAAATTTTTAAACNGAATATCAATATCAACAT
TARGET=144,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3430
PRIMER_LEFT_PENALTY=0.312895
PRIMER_RIGHT_PENALTY=0.030114
PRIMER_LEFT_SEQUENCE=GCGAAGACGTGACAGTAATTGA
PRIMER_RIGHT_SEQUENCE=GGCGTTAATCCTGAAATGAAAG
PRIMER_LEFT=4,22
PRIMER_RIGHT=246,22
PRIMER_LEFT_TM=60.313
PRIMER_RIGHT_TM=59.970
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.3476
PRIMER_LEFT_1_PENALTY=0.312895
PRIMER_RIGHT_1_PENALTY=0.034674
PRIMER_LEFT_1_SEQUENCE=GCGAAGACGTGACAGTAATTGA
PRIMER_RIGHT_1_SEQUENCE=AAACGGAAAGAGAGGAATAGGG
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=204,22
PRIMER_LEFT_1_TM=60.313
PRIMER_RIGHT_1_TM=59.965
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.3476
PRIMER_LEFT_2_PENALTY=0.312895
PRIMER_RIGHT_2_PENALTY=0.034674
PRIMER_LEFT_2_SEQUENCE=GCGAAGACGTGACAGTAATTGA
PRIMER_RIGHT_2_SEQUENCE=CGGAAAGAGAGGAATAGGGTTT
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=201,22
PRIMER_LEFT_2_TM=60.313
PRIMER_RIGHT_2_TM=59.965
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=0.3812
PRIMER_LEFT_3_PENALTY=0.312895
PRIMER_RIGHT_3_PENALTY=0.068302
PRIMER_LEFT_3_SEQUENCE=GCGAAGACGTGACAGTAATTGA
PRIMER_RIGHT_3_SEQUENCE=TGGAAACGGAAAGAGAGGAATA
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=207,22
PRIMER_LEFT_3_TM=60.313
PRIMER_RIGHT_3_TM=60.068
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.3881
PRIMER_LEFT_4_PENALTY=0.312895
PRIMER_RIGHT_4_PENALTY=0.075241
PRIMER_LEFT_4_SEQUENCE=GCGAAGACGTGACAGTAATTGA
PRIMER_RIGHT_4_SEQUENCE=ATAGGGTTTGGGGAATGAGAGT
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=188,22
PRIMER_LEFT_4_TM=60.313
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_03408 Ps_contig_mira-and-tgicl-ass_franssen_29882
SEQUENCE=TACGTACGGGGGTTGGCAGAGATTGGTTTGGTTAACGAACGCTTCAACGGCAAAATTTCCCGGGCTACAAGGCTTCCTGTCTCTCTCTCTCTTTCATCTCCATGCGCGACAATTCCCATTTTCTCACTCATAATTTCCATCTCCTCCAAAAGGATGCGTAATTGAAGGGGGAATTGGCAATAACTGGAGGAGGAGAAGTAGGGAGGGTGTGTGGGTGAGAGTGATTGGGGTCCTAAGGTGGAAGATCTGGCTAGGGTTTTCGTTTTTCATTGGAGATTGAGGTGAAATTAAGGAGAAGATGAGCGCTTCTAGGTTCATCAAGTGTGTTACTGTTGGGGATGGAGCTGTGGGTAAAACATGTTTGCTAATTTCCTACACAAGCAATACCTTCCCTACTGATTACGTGCCAACTGTCTTCGACAATTTCAGTGCAAATGTGGTTGTGAATGGAAGCACTGTTAATCTGGGATTGTGGGATACTGCAGGACAAGAGGATTATAACAGATTAAGACCTTTGAGTTATCGTGGTGCCGATGTTTTCATTCTGGCTTTCTCCCTCATTAGCAAGGCCAGTTATGAAAATGTTTCCAAAAAGTGGATTCCAGAGTTAAAGCATTATGCACCTGGTGTTCCCATTATACTGGTTGGCACTAAGCTTGATCTTCGGGATGACAAGCAGTTCTTTGTTGACCATCCTGGTGCTGTGCCCATTACCACTGCTCAGGGAGAAGAGCTTAGGAAGCTGATCAATGCACCTGCTTATATTGAATGCAGTTCGAAATCACAGCAGAATGTGAAAGCAGTGTTTGATGCAGCCATAAGAGTTGTCCTTCAACCACCTAAGCAAAAGAAAAAGAAGAGTAAAGCACAAAAGGCCTGTTCGATATTGTGATTTGCTAGGTTGGTCTAAAAATTTGACAACCATTCAATCTGTAATTCTCTCCTCTTCTTCATTTTCTTCATTCCTACGCAGTTGAAGAAGTTGGAGTCTATGTGGGTGTTCGTTCGTCTAACATACTCTAGATCTCCTTGAAGCATTAAGGTGGTTGATGCTTTGTTTTTCTTGGAATTATTGTGAATTCTAATTGTCAAATTTGCATTTCATGGTTCCCACCTTTTTCTTAACTTACGTTTTATGTATTAATAGACTGGAATGTCCCAGTTAGGATGACTACCTTGTTTGCTTGAAGATATATATAATATCTAAATTATTTTGTATTTCGTAATAATGTTTGATGTGTGCAAAGATTCCTAGTCTATTTCTTTAGGTTAAG
TARGET=80,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1928
PRIMER_LEFT_PENALTY=1.158747
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=GTTTGGTTAACGAACGCTTCA
PRIMER_RIGHT_SEQUENCE=CTCCTCCAGTTATTGCCAATTC
PRIMER_LEFT=25,21
PRIMER_RIGHT=191,22
PRIMER_LEFT_TM=60.159
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=1.2816
PRIMER_LEFT_1_PENALTY=1.158747
PRIMER_RIGHT_1_PENALTY=0.122861
PRIMER_LEFT_1_SEQUENCE=GTTTGGTTAACGAACGCTTCA
PRIMER_RIGHT_1_SEQUENCE=CCCTACTTCTCCTCCTCCAGTT
PRIMER_LEFT_1=25,21
PRIMER_RIGHT_1=202,22
PRIMER_LEFT_1_TM=60.159
PRIMER_RIGHT_1_TM=60.123
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=1.3908
PRIMER_LEFT_2_PENALTY=1.158747
PRIMER_RIGHT_2_PENALTY=0.232060
PRIMER_LEFT_2_SEQUENCE=GTTTGGTTAACGAACGCTTCA
PRIMER_RIGHT_2_SEQUENCE=TAGAAGCGCTCATCTTCTCCTT
PRIMER_LEFT_2=25,21
PRIMER_RIGHT_2=310,22
PRIMER_LEFT_2_TM=60.159
PRIMER_RIGHT_2_TM=59.768
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=1.3908
PRIMER_LEFT_3_PENALTY=1.158747
PRIMER_RIGHT_3_PENALTY=0.232060
PRIMER_LEFT_3_SEQUENCE=GTTTGGTTAACGAACGCTTCA
PRIMER_RIGHT_3_SEQUENCE=AGAAGCGCTCATCTTCTCCTTA
PRIMER_LEFT_3=25,21
PRIMER_RIGHT_3=309,22
PRIMER_LEFT_3_TM=60.159
PRIMER_RIGHT_3_TM=59.768
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=1.3970
PRIMER_LEFT_4_PENALTY=1.362861
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=GTTTGGTTAACGAACGCTTCAA
PRIMER_RIGHT_4_SEQUENCE=CTCCTCCAGTTATTGCCAATTC
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=191,22
PRIMER_LEFT_4_TM=61.363
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_03409 Ps_contig_mira-and-tgicl-ass_franssen_29884
SEQUENCE=TTTGTTGCTAGTATTTGAAGGTAGTAAAGAGATATCTAACCTTATCCAATCAACTACACGAAGGCCACAACATTTACCTTAGCCAATTAACTATAAGATCACTTTATGAACCTTTGGAGTAACCACTGATGTTCTAAAAAACCTCAAACCCAAAGACAATTTATTGTTGTCTAGACCATTCTAGCGCAAATGAATGCCACATTTGAAATTTCTCTGAATGCGCATTGAAGGTGTTCGACTGACCTAAATGACTCTAACTAATAAGAACATATCAGATCGAGAAACATTTTCGTCCTATTTTATGATATTCGTCAAACGCTATCACTTCAACCCAAAAATTTAAAAGGTGTTATATACTAGTACTACTACTAATAAATATAAAAAATATTAAATAAGTTTATTATTATTAATAATAATATTTTGGCTACAAAAAGNGTGAATTCTTTTGACATAAATAATATTGTGTGATGGAACAAGAAACAAATTGCAGTCTAGATTATGCCACCAAAGAAGGGCCATGGATTATAATTTAATCTAAGGGTTAGAAATGAACCAGAAAGCATGAGATGATTCTAGAATACGTGTAACCACATATAACATTATAAACCAACCCTCATCCTTCCCNACTTTCATAAACCCATTTCTTCCTTCATTTTTCGTTACCTACTAGTTTCTCTCTTCTTACCCTCATCCACTCTACTTTCTACTTGTCTTTCTATCCTCCAAATTAGGGTTAGGGTTTCATCTTATCTCTTCACTCTTCACTCATGGCTTCTTCTGCTCAAATACACGGTCTCGGAACCGCTTCTTTCTCTTCCCTCAAAAAACCCTCTTCCATTTCCGGTAATTCCAAAACCCTTTTCTTCGGTCAGCGACTCAATTCCAACCACTCTCCCTTCACCCGCGCCGCATTCCCTAAATTAAGTAGCAAAACCTTTAAGAAGGGTTTCACTTTGAGAGTTGTCAGTGAGAAAGTCGTTGGTATCGATTTGGGAACCACCAATTCCGCCGTCGCCGCCATGGAAGGTGGAAAACCTACTATAATTACCAACGCTGAAGGTCAGAGAACCACGCCATCTGTGGTGGCGTACACCAAGAACGGTGATAGGTTGGTCGGTCAAATTGCCAAGCGGCAGGCGGTTGTGAATCCCGAGAACACGTTTTTCTCCGTCAAGAGGTTTATTGGACGGAAGATGTCTGAGGTTGACGAAGAGTCAAAGCAGGTCTCTTACAGAGTTATCAGAGACGATAACGGCAACGTTAAACTCGATTGTCCTGCTATCGGTAAATCTTTTGCAGCTGAAGAAATTTCTGCTCAGGTTTTAAGGAAGCTTGTTGATGATGCTTCAAAATTTTTGAATGATAAAGTAACCAAGGCTGTGGTTACTGTGCCTGCTTACTTCAATGATTCACAAAGGACTGCTACAAAGGATGCTGGAAGAATAGCTGGTCTTGAAGTTCTTAGAATCATCAATGAACCAACTGCCGCATCCTTGGCTTATGGTTTTGAAAAGGAAAAACAATGAAACTATTTTGGTATTTGACCTTGGAGGTGGTACTTTTGATGTCTCAGTGCTCGAGGTTGGTGACGGAGTCTTTGAGGTGCTGTCTACTTCTGGTGACACACACTTGGGTGGTGATGATTTTGATAAGAGAGTTGTTGATTGGCTGGCTGGTGACTTCAAGAGAGATGAAGGTATAGACCTTTTGAAAGACAAACAAGCTCTTCAACGTCTTACTGAGACAGCTGAGAAGGCAAAAATGGAGCTTTCATCATTGTCTCAGACAAACATCAGTTTGCCATTCATAACTGCCACAGCAGATGGCCCCAAACATATTGAGACCACTCTTACAAGGGCTAAATTCGAAGAGCTGTGTTCAGATCTCCTTGACAGACTTAGGACACCAGTGGAAAATTCATTGAGGGATGCAAAGCTTTCAATTAAGGATATTGATGAGGTGATTCTTGTTGGTGGATCAACACGTATTCCTGCTGTTCAGGAGCTTGTAAAGAAGCTGATTGGGAAGGATCCAAATGTCACTGTTAATCCAGATGAGGTGGTTGCCCTTGGAGCTGCTGTTCAGGCTGGTGTTTTGGCTGGAGATGTTAGTGACATTGTGCTGTTGGATGTTTCTCCACTGTCATTGGGGTTGGAAACTCTTGGTGGTGTGATGACCAAGATTATTCCAAGAAACACTACCCTTCCCACTTCAAAATCAGAGGTTTTCTCTACTGCTGCTGATGGGCAGACAAGTGTAGAAATCAATGTCCTTCAAGGCGAGAGAGAATTTGTTAGGGACAATAAATCTCTTGGTAGCTTCCGTTTAGACGGTATCCCTCCCGCTCCTCGTGGGGTTCCTCAAATCGAGGTGAAATTTGACATTGATGCAAATGGTATTCTATCGGTTGCTGCCATAGACAAGGGCACAGGAAAGAAACAAGATATTACCATTACTGGTGCCAGCACTTTGCCTGGTGATGAGGTTGAGAGAATGGTGAGCGAGGCAGAGAGATTTTCAAAAGAGGACAAAGAAAAGAGGGAAGCAATCGACACCAAGAACCAGGCAGACTCTGTTGTCTACCAGACTGAGAAGCAATTGAAAGAGCTAGGAGAGAAAGTTCCCGCCCCTGTTAAAGAGAAGGTGGAAGCTAAACTGGGAGAACTTAAAGAAGCTATTACCGGTGGTTCGACTCAAACTATTAAAGATGCCTTGGCTGCCCTCAACCAGGAAGTTATGCAGCTTGGTCAGTCTCTATATAACCAACCTGGAGCTGCAGGACAAGCAGGACCTACACCACCAGGTAGTGAGTCTGGCCCCTCAGAATCGTCAGGCAAGGAGGGACCTGAAGGAGATGTGATTGATGCTGATTTTACAGACTCTAAATGAGTTAATAGAGATTATTGAATAGGGATTATTTTTATTTTATGCTTCTCCTGTGCTAAATTCTTAGGGATTTTTGTCAATTGAGTGCGAGCGGATCTTCTCTTAATATAATTAGACTTTATTTGTTTATTATAGGTGCTCATTGACGCACACGTTTCTGTATCTTTTGGAAATTTTGTCTGAATTACATGTATTGTGGATGGAGTTTACAGGCCGAAAATGTTGTAATTTGATATAAGAAAAATAAGATAAATTTGAACCYG
TARGET=398,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03410 Ps_contig_mira-and-tgicl-ass_franssen_29891
SEQUENCE=TTCGCTTGCCGTTGGTGAAAAGTATCAATGTTAGCGGTCACAAGTACGGTCTTGTTTATCCCGGTATTGGTTGGGTTGTCTGGAGGAATCAAGATGACTTGCCGGATGATCTTGTTTTTCATATCAATTATCTTGGATCTGATCAGCCAACTTTCACATTGAATTTCTCCAAAGGATCGAGTCAAATCATTGCTCAATACTACCAATTCATTCGACTTGGATTCGAGGGCTACAAAAATGTGATGGAAAATTGCTTGGCGAACACGAGAATTCTGAAAGAAGGAATTGAGAAAACTGGAAAGTTTAAGATTGTGTCCAAGGACGTCGGCGTGCCACTTGTAGCGTTCTCGTTGAAAGACAGTAGCCGGCACACGGTATTCGAGATAGCAGACCATTTGAGAAAATACGGGTGGATAGTTCCGGCCTACACAATGCCTGCGGACGCGCAACACATTGCGGTCCTGCGAGTGGTGATCAGGGAGGATTTTAGCCGCAGCTTGGCTGAGAGACTTGTTTCTGATATTTGCAAAGTTGTGACTCTCTTGGAGACACTTCCAAGTACCATCTCCTCGAAAAGTGCTCATGTCGCGGTTATCGCGAGTGAGACGAGCGAGGAGGTTAAGAAAGATATTACAGAGACTCAAACTGAGATTGCAAGTTATTGGAAGAAGCTTGCTACAGGAAAGAGAGTTGGAACATGCTAGTGTTGTCAAATGTTTGTTGTGTTTTGTGAGCTACTTTGTTTGTGTCGTACAATTAAATTTAATGTGTGTAAGGATTGTTTCATTGTTACTTTATTTATCTATGTAGAGATATAACCATTGCCAATGTATCTCTAAACCATATATTTATATGAATGAATGAATATGAGTTGTCTTGTTTCCTTAGC
TARGET=853,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03411 Ps_contig_mira-and-tgicl-ass_franssen_29907
SEQUENCE=ACTTCRTCGGGGACCTCTCACTCTCCTCTCTCTACAATCCGTTACACTAACACAGTTTCTTCTTCACACACTGTGTTTAGTGTAATCAACCATGTCTCTTACAGTAGAGAAAACCACCGCTGGCCGTGAGTACAAGGTCAGAGACCTTTCTCAAGCAGATTTTGGCCGTCTCGAAATTGAGTTGGCCGAAATCGAGATGCCCGGACTCATGTCCTGCAGGACCGAGTTCGGACCATCCCAGCCCTTCAAAGGAGCCAGAATCACCGGATCTCTTCACATGACCATCCAAACCGCCGTCCTCATCGAAACCCTAACCGCCCTCGGCGCTGAAGTCCGTTGGTGTTCATGCAACATTTTCTCAACTCAAGACCACGCCGCCGCCGCCATAGCTCGTGACAGTGCTGCTGTATTCGCCTGGAAGGGTGAAACCCTACAGGAATATTGGTGGTGTACTGAACGCGCTTTGGACTGGGGTCCCGGTGGTGGTCCTGATCTAATTGTTGATGATGGTGGTGATGCTACTCTTTTGATCCATGAAGGTGTTAAAGCTGAAGAGCTTTTTGAGAAAACCGGTGAGGTTCCTGATCCTACCACTACCGATAACGCTGAGTTTCAGCTTGTGCTTACCATCATTAGAGATGGGTTGAAGACTGACCCTCAAAGGTACCGTAAGATGAAGGAGAGACTTGTTGGTGTTTCGGAAGAGACTACAACTGGTGTTAAGAGGCTTTATCAGATGCAAGCCAGTGGAACTCTTTTGTTCCCTGCTATTAATGTCAATGACTCTGTCACCAAGAGCAAGTTTGACAACTTGTATGGATGCCGTCACTCTCTTCCAGATGGTTTGATGAGGGCTACTGATGTTATGATTGCTGGGAAAGTGGGTGTTGTCTGTGGATACGGAGATGTTGGAAAGGGTTGTGCTGTTGCCTTGAAGCAAGCTGGAGCTCGTGTGATTGTGACTGAAATTGATCCAATCTGTGCCCTTCAGGCTCTCATGGAAGGATTCCAGGTTCTGACTCTCGACGATGTTGTCTCTTATGCTGATATCTTTGTTACCACCACTGGTAACAAGGACATCATCATGGTTGGTGACATGAAGAAAATGAAGAACAATGCCATTGTTTGTAACATTGGTCACTTTGACAATGAAATTGATATGTCCGGTGTTGAGAACTACCCTGGTGTGAAGCGTATCACCATCAAGCCTCAAACCGACAGGTGGGTTTTCCCTGAGACCAACACTGGCATCATTATCTTGGCGGAGGGTCGTTTGATGAACTTGGGATGTGCCACTGGACACCCTAGTTTTGTGATGTCCTGCTCTTTCACCAACCAGGTCATTGCTCAACTAGAGTTGTGGAATGAGAGGAAATCTGGAAAGTATGAAAAGAAGGTTTACGTTTTGCCTAAGCATCTTGATGAGAAGGTGGCTGCACTTCACCTTGGCAAGCTTGGAGCCAAACTCACCAAGCTTACCCAATCACAGGCTGATTACATCAGTGTGCCAGTTGAGGGTCCTTACAAGCCAGCTGCCTACAGGTACTGAAAGGTGATCAAGGTGGTGATGAGATTGAGGGACAAGAAAATTGATTTTATGAAACCTTTTGATTGTTTAATTTTTCCTTATTTTGGGGTGTTAGACTTTTTTCAGAATTGTGGCTGTAGAATTTTAGGTTTTTGTGATGTTAGGGTGGGATGGGTATGAGGAAGGTAACCTATTGGTTTCTGTTGATTGAGACCAAATGGGTTTGAATAATGCTTTGGTTGGGGTTGTGTGAGAAGTATGCCTTTAATGTTTTTAAAAAATGAGTAAAAAGTTTTCTGGCGTACCTACTACTATCTTGATTTCGAAGTATCATGTTCATGTTCATGTTCACACGCGTACTAGAATGGTTGGTTTCTTTTTGTATTTTTTTATCATCTTCAGTAGAAAACAAAATGTCGGCTTATGACAGCGAAANRTTGGCGTTATAGTCCAAACTTATTTTCCTCTTTATTGAA
TARGET=373,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03412 Ps_contig_mira-and-tgicl-ass_franssen_29910
SEQUENCE=ACTTTGNTTGAAGAAGTGAAAATCCATTTCACAATTAAGTAAAGTCGACAAACAATAGATAAGTGTTTTCTCAATATCTCTTCATACCAAATTGTATATCAAATTGTATATGTCACTCAACCATTAATTGACAGATTCATGCCTCCAAGAAATTAAATTCCATAACATACAAAAGATCTTTGACATAAGGTCAGAAGAAGAATGAAATAAGCACTTTACAGTTTACACTGAATCAACAACAACACCTTCAACCTGCTGCTTTGCCAAGTATCTTTCCCTTCTTTCTTTCCATTCATCTATAACAAGTCCCTCTCCAGCAACTCGTGGACCGGTCTCCTCTGGCGGTTTCTTTCGTGCTTCCAATGCAGCCTCCGCCTCAGCTAACTGACGTTTAAGCTTATCCATGGCAGGGCTGTTGCGGTCAGGGTCAAGAAAAACAACCCGAAGAATTTGCTCTACAGCTACTTGATCTCTTTGCTCTTCGAACAGCTCAGGCACATAGTCCATTTCACCTTGCACTTTTAGGCTCATGATTTTGAACTTAACCTTGCTTTTTTGATCAGTTGGTTTCTCATTGTTCTCCGGTGGTTCAACGAACTTGAACACAGTGGCAATTATACTTTCACCAGGTGAAAGAATGCCGCCCGGAGGGCGCATGTAACAACTTTTAGGCGCAGTTGTTTGAAACTTGAAAGCTACATGAGACTTGCAAGTGTTTTTAATTGTGATTGCACTCCTAACTTGCTTACCAGGTTCATAAGGAAAATAGAGCTTGTTGGGAGGATCAAGACGAAGCCTTCTCCTTGTAGGAAGCAAAGATTTAGCCACAGATGAAACCGTAGCTGAAGATTGAGGAATAGATCTGTCAATAGAATGAACACTCTGGCTTTGGTGATGAACATTATGCATGTAAGATGTAGATGAAGAAGTAGTAGTAGTTGATGAAGATGAAGGATTGTGACTCGTTTGCCAAAACGGCATTCTACAAAAGTTCCAAACTTTTCCATCAGATCCTGGCTTTTCACTCTCCACCTCCATTTTTCGCCGGAATCTCACCGGAGACGGAATATTCCGGCAAATGGGTCACTGAATTGGTGGAAAAGTTTTAAGCTTTTTGCTAAATAAAGTGATGGGTCTTTTGAAGCAAAAATGGTGTGAAACAACGCAAAGGTCGAAACCACCAACGTTGTTGTTGTATTTCNYYCACAAACAATTAGTTTTTGT
TARGET=928,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03413 Ps_contig_mira-and-tgicl-ass_franssen_29913
SEQUENCE=AAGCAGTGGTATCAACGCAGAGTACGTCGGGGGATTTAGGTTTGTAACAATTCGTGATCCCTCTCTCTGCGGCTGTCACTGATCTGCGATTTCACCATGGCTCCAAAGAAGGAAAAGGCTCCTCCACCGTCCTCCAAGCCCGCCAAGTCTGGTGGTGGCAAGCAGAAGAAGAAGAAGTGGAGCAAGGGTAAGCAGAAGGAGAAGGTGAATAACATGGTGTTGTTTGATCAGGGAACTTATGATAAGCTTCTCACTGAGGCTCCCAAATACAAGCTCATTACTCCTTCGGTTCTCTCTGATCGTTTGAGGGTTAATGGATCACTTGCAAGGAGAGCAATAAGAGATTTGATGGCTAAAGGTTTGATCAGGTTGGTATCATCCCACTCGAGCCAACAGATTTACACCAGAGCTACAAACACCTAGTTTTGTTGTATTGGTATTTTGATGACTCTGATCAAGTATTTTTCTCTATCGATGGGCATGTACCTCATTCTAGTTTTACCTTTTGCTAAGTTAAATTGATGGTTTGTCTCTTTTTATCATTGGTAACATACTTGACTTATATCTTATTGTTTTTATTTATTGAGCTAGCGTCTATTTGATTAAAACNACT
TARGET=164,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4485
PRIMER_LEFT_PENALTY=0.334016
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=GCAGTGGTATCAACGCAGAGTA
PRIMER_RIGHT_SEQUENCE=TCACCTTCTCCTTCTGCTTACC
PRIMER_LEFT=2,22
PRIMER_RIGHT=207,22
PRIMER_LEFT_TM=60.334
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.5332
PRIMER_LEFT_1_PENALTY=0.418705
PRIMER_RIGHT_1_PENALTY=0.114470
PRIMER_LEFT_1_SEQUENCE=AACAATTCGTGATCCCTCTCTC
PRIMER_RIGHT_1_SEQUENCE=TCACCTTCTCCTTCTGCTTACC
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=207,22
PRIMER_LEFT_1_TM=59.581
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.5506
PRIMER_LEFT_2_PENALTY=0.334016
PRIMER_RIGHT_2_PENALTY=0.216588
PRIMER_LEFT_2_SEQUENCE=GCAGTGGTATCAACGCAGAGTA
PRIMER_RIGHT_2_SEQUENCE=AACCGAAGGAGTAATGAGCTTG
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=290,22
PRIMER_LEFT_2_TM=60.334
PRIMER_RIGHT_2_TM=59.783
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.5812
PRIMER_LEFT_3_PENALTY=0.334016
PRIMER_RIGHT_3_PENALTY=0.247232
PRIMER_LEFT_3_SEQUENCE=GCAGTGGTATCAACGCAGAGTA
PRIMER_RIGHT_3_SEQUENCE=CCTTCTCCTTCTGCTTACCCTT
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=204,22
PRIMER_LEFT_3_TM=60.334
PRIMER_RIGHT_3_TM=60.247
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.6019
PRIMER_LEFT_4_PENALTY=0.334016
PRIMER_RIGHT_4_PENALTY=0.267873
PRIMER_LEFT_4_SEQUENCE=GCAGTGGTATCAACGCAGAGTA
PRIMER_RIGHT_4_SEQUENCE=AGTTCCCTGATCAAACAACACC
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=236,22
PRIMER_LEFT_4_TM=60.334
PRIMER_RIGHT_4_TM=60.268
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_03414 Ps_contig_mira-and-tgicl-ass_franssen_29914
SEQUENCE=CGAAACCCGTTTATTATCTGAACCGACCCGCTCTCAATCTCTTCTATAAAACCCTCTCTTACATCCCCAATTTCTCATCCTTCATTTCTTTCGTACGCAAACCCGATCCTAGTTTCCACTAATTCACAATTTTCACGATCACATCACAATTTTCACAACCACATCACAATTTTCGATTCCTTCTCCTCCACAGCGTAATCGTCGCAATTCACCATGGGTTACGAAGACAATCCGTATCGTGATGAGGATGGCGAACCGTTGATGGACTACGACGATGTGCAATCCGACGGCGAAGCGACACCGGAGCCGCAACAGCTTGACGATTTTGAGGAGGAAGACGTTGATGACTGGCGTGGTCGAGAGAGGTCACAAACGCCGGTGCATGACACCGATTCGTCAAAATCGAAGCCTAGGAAGAGGCTGATTAAAAAGAGCGACACCGGGAAACAGTCAGGGAAACTGTCGGTTGCACCGGAACTCGAAGATGAGTTGGAGGAGGACGGTTATCGGGGGATGGCTGATGAGGAAGAAGGGAGGAAGAGGAAGAAAGGGAAGGATAGTGGATCTGGGAAGAAGGAAAAGAGGCTGAAAGGGGGGGAAAAGGAGGTATGGTGAAAGTAGCGGTGCAAAGAGTGGGTCTAGATTTGGGGGTTCCAAGAAAGCGTTTGGTGGTAAGGCTGGGAATGATCATGATGGTGAAGTTAAGGAGATGTGGGATACAATTGCTGGAGGGGATTCTGAGGATGATCACGAGGGTAACAGGAATATGGATGATGACAACTTTATAGATGATACTGGAGTAGAACCTGCTTTATATGGTTATGATGAACCACGGTCTCCTGGTGATGCTCCTCAGGCAGAGGAAGGTGAGGAGGATGATGAAGTTAAGGATCTTTTCAAAGTGGGTAGGAAGAAGAAGAAGAATGAGAGGAGTCCTGCAGAAATTGCTTTATTAGTTGAGAATGTCATGGCTGAGCTTGAGATTACAGCTGAAGAGGATGCTGAACTTAATAGACAGTTTAAGCCTGCTATTAATAAACTCAAGAAGTTGCCTCTTCTCCAAGAAGTCCTCTCGAAGAAACAGCTTCAACTTGAGTTTTTAGATCATGGAGTGCTAACGTTATTGAAGAATTGGCTTGAGCCACTTCCTGATGGAAGTCTGCCAAATATTAACATAAGGACAGCAATTTTGAAGATTTTGAATGATTTTCCCATAGACCTAGAGCAGTTTGATAGAAGAGAACAGCTGAAGAGGAGCGGTCTTGGAAAGGTGATTATGTTTTTATCAAGGTCCGATGAAGAAATCAATGTAAATAGGAAATTAGCCAAAGATTTGGTTGATAAATGGAGCCGGCCTATTTTTAACAAGAGTACCCGGTTTGAAGACATGCGTAATGTTGAGGATGATAGGGCTCCATACAGGAGGCCATCTGTTAAAAAGCCAGCAAATAAAGCTGCGGGGATGGAATCTAGAGATGGCGATCTTGATTTGGATCTTTCACAGCCTAGGGCTGGACAGTCGTCTTCCAGGCAACATGCATCAAGGCCAGAAGCAACACCTTTGGATTTTGTTATCCGCCCCCAATCTAAAATTGACCCTGATGAAATTAGAGCACGTGCAAAACAAGGTTCACAAGACCAGCATCGAATGAAGATGAATAAGAAATTGCAGCAGTTGAGGGCACCAAAAAAGAAGCAGCTTCAGGCTACAAAACTGAGTGTGGAAGGTCGTGGTATGGTCAAATACTTCTAAATGTGACACCAATACTGGATGGATTTGTTTGTTGTACTGAAGAATTTGATGGGGGATGTCATCAAAATACACTTCCCTTTACTGCATGTATCGTGTATTCTTGAATTGGAAAAGTGAAAGCCATAGAACATTGGGCGATTATTTACAATTTTGTGTTTGTTATTTCCTTAGTCTCAAAATAATGAAAGGTTTGTCATCAATTTGATGTAATGAAAATGTGTATTCTGCATACGTTATTGGTAGAACTAAAACGCGATTGAATCTCAGCAGTACCATCATTTTTTAAAC
TARGET=910,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0828
PRIMER_LEFT_PENALTY=0.073866
PRIMER_RIGHT_PENALTY=0.008930
PRIMER_LEFT_SEQUENCE=GAAGGTGAGGAGGATGATGAAG
PRIMER_RIGHT_SEQUENCE=TTTCTTCGAGAGGACTTCTTGG
PRIMER_LEFT=862,22
PRIMER_RIGHT=1080,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.991
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.3150
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.179006
PRIMER_LEFT_1_SEQUENCE=GGTGAAGTTAAGGAGATGTGGG
PRIMER_RIGHT_1_SEQUENCE=AGCTGTAATCTCAAGCTCAGCC
PRIMER_LEFT_1=694,22
PRIMER_RIGHT_1=990,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.179
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.4027
PRIMER_LEFT_2_PENALTY=0.073866
PRIMER_RIGHT_2_PENALTY=0.328883
PRIMER_LEFT_2_SEQUENCE=GAAGGTGAGGAGGATGATGAAG
PRIMER_RIGHT_2_SEQUENCE=TCAAGTTGAAGCTGTTTCTTCG
PRIMER_LEFT_2=862,22
PRIMER_RIGHT_2=1094,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.671
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.4305
PRIMER_LEFT_3_PENALTY=0.073866
PRIMER_RIGHT_3_PENALTY=0.356592
PRIMER_LEFT_3_SEQUENCE=GAAGGTGAGGAGGATGATGAAG
PRIMER_RIGHT_3_SEQUENCE=GAAGCTGTTTCTTCGAGAGGAC
PRIMER_LEFT_3=862,22
PRIMER_RIGHT_3=1087,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.643
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.4358
PRIMER_LEFT_4_PENALTY=0.073866
PRIMER_RIGHT_4_PENALTY=0.361969
PRIMER_LEFT_4_SEQUENCE=GAAGGTGAGGAGGATGATGAAG
PRIMER_RIGHT_4_SEQUENCE=GGAGAAGAGGCAACTTCTTGAG
PRIMER_LEFT_4=862,22
PRIMER_RIGHT_4=1060,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=59.638
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_03415 Ps_contig_mira-and-tgicl-ass_franssen_29919
SEQUENCE=TRTTATTGAGAAAATTTAAGCTTTAATATATTGATTGCAATTTTCAATCTTGTAGACTGTCATTCAAAGAGTTTGTTTGGACAAAATGGCTTTCAACCAACTTAGAGATGGGTCTCTATTGGCTTTTAACATTTATTTCAAAAACAAAAAGGCAATATAAACTTAAAAGCAGAGTTTACAACAGGGGGCTCGGGTTTTATTATTAAGAGATATAACTAAGATAATCCCCCACATTTGAATGAATAAAATTTGTTGGTAAATTGTCAAAGCAAACACAATCACACTTCTAAGAATTGGATTTCAAGCTTTCGGCTGAAGTGACTTGATGTGTGCAATTTGCTTTCCAGGGTTCAAACCCTTTGGACAGGCACGAGCACAGTTCAATATGGTGTGGCAGCGATACAGCTTGAATTCATCATTAATGGATTCCAATCTCTCTGTAGTGTACTCGTCACGGCTGTCACTTATCCACCTATTAGCGTGGAGCAAAGCAGCAGGTCCAAGATAAGACTCAGGGTTCCACCAATAACTAGGGCAAGACGTGCTACAGCATGCACACAATATACACTCATACATCCCATCAAGCTTAGCACGATCCTTCTTACTCTGAAGAATCTCCTTCCCCGGTTCCGAAGGTGGATTCTTCCGCTTCAACCACGGTTCAATACTCTTATACTGATTATAAAAATTCGTCATATCAACCACGAGATCCTTCACCACAAACATATGAGGCAACGGCGTTATCGTCGTATCCGAACCTTCGGAAGGAATCTTCGTCAAACACGCGAGTCCATTACAACCGTCGATGTTCATCGCACACGAACCACAGATCCCTTCACGGCACGATCGACGGAAAGTGAGACTTGGATCGATTTCGTTCTTGATCTTAATAAGGGCGTCCAGAACCATAGGCCCGCACTCCTTTAGGTTGATTTCGTATTCTTTTAGCTCGGGCTTTGAAGGAGTGTCGGGATTCCAACGGTAGATTTGGAATTTCTTTAGAGCGGTGGTGTCGCTGGCTTTGGGCTGTACCTGCTGGGCTTGGGCTTCGGAGGCGTGGGCTCTGAGTAGGGTTAGCCTCGACGCTGGGGAAGATGATGAGAGCCTGTTGATGGCTCTCTTGAGCATCGTCGAAGCCATTGTTGAGAGAGAGAGAAGTGGTGACGTGGTGGTGATGGAAATGGGTTGAGATGATTTTAGTGAGTGCGATGCCCCCGTACTCTGCCTTGATAACACTGCTT
TARGET=1145,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03416 Ps_contig_mira-and-tgicl-ass_franssen_29921
SEQUENCE=AACTTYTTGAAGCTCTTTTTTAATATTCATTCAACTAAACATTCAGTTCGATTCATTGGAATATGAATACAATAACACCCACCCAGAATAACCAACTAACAACAACTGTTTACTTATTTATTTATACATTAACATTTCAGAATTACATGACCATATAATTTCTTGCACACGCCACAAAAAGGAAAACATTATAACCAAGAAAGCACACGATTTAAAGCAACAGAGTAACAGACTCAGTCTAAGTTTAACCAGAAATCTCAATAGACTTAACCTCAGCCTTCTTTATCTCTTCTTTTGGAACTGTCACTGTGAGAACGCCGTTCTCCATGGAAGCTTTCACTTTATCCATTTTAGCATTCTCAGGTAATCTGAACCTTCTCAAGAACTTTCCACTGCTACGTTCCACGCGATGCCATTCATCATTCTTATCTTCTTTCTCGACACTTCTCTCTCCGCTTATCTGTAGAACCCTATCATCTTCAACTTCAACTTTCACTTCCTCCTTTTTCAGGCCAGGAAGATCAGCCTTGAAAACATGAGCTTCTGGGGTCTCCTTCCAGTCAACTCGAGTGCTCACAAAAGCAGAATTTTCACGAGGGAATGAAGCGGAAAGTGAAGAATTCGAAAATGGAAAGTCCTTCAAAGGATCCCAGACGTCCAGGGAGAAAGGATCGAAAACGTTGCTTCTTCGACCACTAAAGAAACTTGGAATCAGAGACATGTTTGATAGAGTAATAATTGAGAACTCTGTATAGAACTTGATAATTGACTGTAAAATGCTATCGATGCTTAGTGTCCCCGNGTACTCTGCGTTG
TARGET=114,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03417 Ps_contig_mira-and-tgicl-ass_franssen_29923
SEQUENCE=CCCACCGGTTATTCTCGAACCATCCGCCACCTTTTCTTCTTCTTCTTCTTCTCCAACCGATCGCAGGCTAAACACCCTCGCAAGACACCTTTCTCAATTTCCAGATCATAATCAAATGGCTTCTCATATTTCTCCTTCTCCAACAGCTTCTTCCGATTCCGTTTTCGCTCACATCGCTCAAGCTCCCGAAGATCCTATCCTCGGGGTAACTGTTGCTTACAACAAAGATCCAAGTCCTATTAAGCTCAACTTGGGCGTTGGTGCTTACCGAACTGAGGAAGGTAAACCCCTTGTGTTGGATGTAGTGAGGCGTGTTGAACACCAACTGTTGAACGACTTGTCACGCAACAAGGAGTATATTCCAATTGTTGGGCTAGCTGATTTTAACAAATTGAGTGCTAAGCTTATTTTTGGCGCAGACAGCCCTGCTATTCAAGAGAACAGGGTTAGCACTGTTCAAGGCTTATCTGGTACTGGTTCTTTAAGAGTTGGGGGTGAATTTTTGGCTAAGCACTATCACCAACGGATTATATACTTGCCACAACCTACTTGGGGAAATCATACAAAGATTTTTGCCTTGGCAGGTTTAACTGTCAAAACATATCGCTACTATGCTCCAGCAACACGGGGGCTCGACTTCCAAGGACTTCTAGAGGATCTTGGTTCTGCACCATCTGGATCTGTTGTTTTGCTACATGCATGTGCACACAACCCTACTGGTGTTGATCCAACGCTTGAACAGTGGGAGCAGATTAGACAGCTCATAAGATCAAAATCTCTGTTACCTTTCTTTGACAGTGCTTATCAGGGTTTTGCCAGTGGAAGTCTAGATGCAGATGCACAGCCTATTCGTTTGTTTGTTGCTGATGGTGGTGAATTGCTGGCAGCTCAGAGTTATGCAAAGAACATGGGTCTTTATGGTGAACGTGTTGGTGCCTTGAACATTGTCAGCAAGTCAGCTGATGTTGCAAGCAGGGTTGAGAGCCAGCTGAAACTTGTGATTAGGCCCATGTACTCAAGTCCTCCCATTCACGGTGCATCCATTGTGGCTGCCATTCTCAAGGACCGGGATCTGTACAATGACTGGACTATTGAATTGAAGGCAATGGCTGATCGCATTATCAACATGCGGCAACAACTTTTTGATGCTTTACGCACTAGAGGTACACCAGGTGATTGGAGTCACATTATCAAGCAGATTGGAATGTTTACCTTCACAGGATTGAATCCTGAGCAAGTTTCCTTCATGACTAAAGAGTACCATATCTACATGACTTCTGATGGGAGGATTAGCATGGCTGGTTTGAGCTCCAGAACAGTCCCACATCTGGCGGATGCAATACATTCAGCTGTTACCGGAGTTGCCTAAATTCTACCTACTGTTTTCAAATCACACCACTGATGGTGGATATTTTTGTGTAATAATTGGCTCATCAACAATTAAACCTTAGTATAATTAAACAATTAAGCCTTGTTTTATGAGAATGACAAACAACTTTTTGCCCACAGGGCTCATGATGCCTCCCTTATAGAGCATTACTAGGTTCCGATCAGGGGAATTTTTAGTTTTATGATTTGAAAAATTTTAAACTTCATGTAATTTTAATTTGAGATTTGAGTTTCAAATGATTTTAATACAAANAANTTTTTCTACGTAAAA
TARGET=34,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2034
PRIMER_LEFT_PENALTY=1.201078
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=ACCGGTTATTCTCGAACCATC
PRIMER_RIGHT_SEQUENCE=GAAGCTGTTGGAGAAGGAGAAA
PRIMER_LEFT=3,21
PRIMER_RIGHT=149,22
PRIMER_LEFT_TM=60.201
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=147
PRIMER_PAIR_PENALTY_1=1.2199
PRIMER_LEFT_1_PENALTY=1.201078
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=ACCGGTTATTCTCGAACCATC
PRIMER_RIGHT_1_SEQUENCE=ACATCCAACACAAGGGGTTTAC
PRIMER_LEFT_1=3,21
PRIMER_RIGHT_1=302,22
PRIMER_LEFT_1_TM=60.201
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=1.2965
PRIMER_LEFT_2_PENALTY=1.201078
PRIMER_RIGHT_2_PENALTY=0.095396
PRIMER_LEFT_2_SEQUENCE=ACCGGTTATTCTCGAACCATC
PRIMER_RIGHT_2_SEQUENCE=ACAGTTACCCCGAGGATAGGAT
PRIMER_LEFT_2=3,21
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=60.201
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=1.3268
PRIMER_LEFT_3_PENALTY=1.201078
PRIMER_RIGHT_3_PENALTY=0.125755
PRIMER_LEFT_3_SEQUENCE=ACCGGTTATTCTCGAACCATC
PRIMER_RIGHT_3_SEQUENCE=AGGGGTTTACCTTCCTCAGTTC
PRIMER_LEFT_3=3,21
PRIMER_RIGHT_3=290,22
PRIMER_LEFT_3_TM=60.201
PRIMER_RIGHT_3_TM=59.874
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=1.3427
PRIMER_LEFT_4_PENALTY=1.201078
PRIMER_RIGHT_4_PENALTY=0.141638
PRIMER_LEFT_4_SEQUENCE=ACCGGTTATTCTCGAACCATC
PRIMER_RIGHT_4_SEQUENCE=AACACAAGGGGTTTACCTTCCT
PRIMER_LEFT_4=3,21
PRIMER_RIGHT_4=296,22
PRIMER_LEFT_4_TM=60.201
PRIMER_RIGHT_4_TM=60.142
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_03418 Ps_contig_mira-and-tgicl-ass_franssen_29928
SEQUENCE=GTTACCAGATAAATACGAATAAAANTAACTAAAAAAAAAATGAAAATTTGTTATAAGAACATAGTTCAGTATAAAGTTGAACTCAACTAGACAACCATACAAAGTGGAACGAGGTATCTGGACAGGACACGATACAATTCTTATCAACATTTATTTGATATAAAACAGGCCGACATACATACATACATAGATAAACATCCTCAGCGTCCAAACGGATACGGCGACAAAACAGCGTTCAAAACGTCTAGCTCAACCACCGATACCAACAGCTACTTTCACTTACTTATGTCACAAGGCAAAAAGCATCATTTTTAACCAATTCATTTGGCTAGAATTGGAACACCACATGCTGCAGGAGCATGCTTACCATCCTTAAAGACAAGGTTTCCTCTAACAAAAGTGTCCAAAACTTTTCCTGATAACCTTCTTCCCATATACGCAGACAGGCTAGGATGTTTTAGGAAAACAGGATAATCGTCATCAAGGTCAAACTCCACCTCTGGCTGCCACACTACAATATCTGCATGGTTTCCAATTGCAATGGCTCCCTTTGATTCTAACCCTGCAAAGGTTGCAGGTTTCTGGCTCCATAATAATGATAACTGTTCTAAGGTTAACCCGTGTTTCTTCCCAGATGACCATGTGACTGGAAGATCAAACTGCAAAGACGATATGCCACCCCAAGCCTTCAAGAAGTCACCTTCCTTAAGAAGCTTGAGTTCAGGCACAGTTGGTGAATGATCAGAACTTAATAGGTCAATGTGTCCATCCAGTACGGCCTCCCATAACTTTTCTTTGTTCAATGCATCACGAATGGGTGGGGAACACTTGTACCGAGTATCTCTGTCTGGTATCTCTTCAGATGAAAATGTTAGGTAATGAGGGGCTGTTTCGACGCTTATGCTGTCCCCACGACGTTTTGCTTCCTTAATAAGGTCTAAGGAAGCACTTGAATCAGACAAGTGAACAATGTGAACATGTGCTCCTTCTAAAGTACCGCCAATTCTAGTATCCTTTGTAACATCTACAAGTTCTTTAATGGCTGCCTCTTCCCATGAAGGTGGTCTAGTGACGAGATATGTATTGTAAGCGTGCGGGTCACCGTTACCTTTAAGTTCCAAATTACTTTTAGAATCTTGTTGAATCTCGGAATGCACAACTAAAGGTCTTTTATATTTTGCCAACACAGACAATCCCTCCTTGATATGTTCAATAGTGGTCATCGGAAAGTCATTGATTCCTGAAGGGCACATAAAAGACTTTACACCAAGAACACCAGCATCTAAGAGACCTTCAAGAATACTGGTATTTTGTGCATTTTCAGGGATCAAGCCTCCCCAAAAACCAACATCAACATAGATTTTATTCTCTGCAGCTTCAAGCTTCAGTTCCAGTGTTTCCTTAGACACGGTTGTTGGGTGATTGTTTAGAGGCATGTCAACCACTGTAGTTACACCTCCAGCAGCAGCAGCTCTGGTACCAGTGACAAATCCCTCCCATTCGGTTCTGCCGGGCTCATCAAGATGTACATGCACATCAATCAAGCCAGGCATGATAAAAGCATCTCCATAATCAATCACTTGTTGAACAGAACTCCCCTGCTTGCCATATCCTTCCACAATAGATGCTTATTTTTCCCTTCTTATTTTATCTCCACTGATCCAAGAAATGATTCCTTGTAGAAACG
TARGET=173,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0518
PRIMER_LEFT_PENALTY=0.001315
PRIMER_RIGHT_PENALTY=0.050443
PRIMER_LEFT_SEQUENCE=GAACGAGGTATCTGGACAGGAC
PRIMER_RIGHT_SEQUENCE=AGTAGCTGTTGGTATCGGTGGT
PRIMER_LEFT=106,22
PRIMER_RIGHT=273,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.950
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.0528
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.050443
PRIMER_LEFT_1_SEQUENCE=AAAGTGGAACGAGGTATCTGGA
PRIMER_RIGHT_1_SEQUENCE=AGTAGCTGTTGGTATCGGTGGT
PRIMER_LEFT_1=100,22
PRIMER_RIGHT_1=273,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=59.950
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=174
PRIMER_PAIR_PENALTY_2=0.0816
PRIMER_LEFT_2_PENALTY=0.001315
PRIMER_RIGHT_2_PENALTY=0.080323
PRIMER_LEFT_2_SEQUENCE=GAACGAGGTATCTGGACAGGAC
PRIMER_RIGHT_2_SEQUENCE=TGGTTGAGCTAGACGTTTTGAA
PRIMER_LEFT_2=106,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=0.0826
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.080323
PRIMER_LEFT_3_SEQUENCE=AAAGTGGAACGAGGTATCTGGA
PRIMER_RIGHT_3_SEQUENCE=TGGTTGAGCTAGACGTTTTGAA
PRIMER_LEFT_3=100,22
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.1171
PRIMER_LEFT_4_PENALTY=0.001315
PRIMER_RIGHT_4_PENALTY=0.115769
PRIMER_LEFT_4_SEQUENCE=GAACGAGGTATCTGGACAGGAC
PRIMER_RIGHT_4_SEQUENCE=TAAGGATGGTAAGCATGCTCCT
PRIMER_LEFT_4=106,22
PRIMER_RIGHT_4=374,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=60.116
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_03419 Ps_contig_mira-and-tgicl-ass_franssen_29928
SEQUENCE=GTTACCAGATAAATACGAATAAAANTAACTAAAAAAAAAATGAAAATTTGTTATAAGAACATAGTTCAGTATAAAGTTGAACTCAACTAGACAACCATACAAAGTGGAACGAGGTATCTGGACAGGACACGATACAATTCTTATCAACATTTATTTGATATAAAACAGGCCGACATACATACATACATAGATAAACATCCTCAGCGTCCAAACGGATACGGCGACAAAACAGCGTTCAAAACGTCTAGCTCAACCACCGATACCAACAGCTACTTTCACTTACTTATGTCACAAGGCAAAAAGCATCATTTTTAACCAATTCATTTGGCTAGAATTGGAACACCACATGCTGCAGGAGCATGCTTACCATCCTTAAAGACAAGGTTTCCTCTAACAAAAGTGTCCAAAACTTTTCCTGATAACCTTCTTCCCATATACGCAGACAGGCTAGGATGTTTTAGGAAAACAGGATAATCGTCATCAAGGTCAAACTCCACCTCTGGCTGCCACACTACAATATCTGCATGGTTTCCAATTGCAATGGCTCCCTTTGATTCTAACCCTGCAAAGGTTGCAGGTTTCTGGCTCCATAATAATGATAACTGTTCTAAGGTTAACCCGTGTTTCTTCCCAGATGACCATGTGACTGGAAGATCAAACTGCAAAGACGATATGCCACCCCAAGCCTTCAAGAAGTCACCTTCCTTAAGAAGCTTGAGTTCAGGCACAGTTGGTGAATGATCAGAACTTAATAGGTCAATGTGTCCATCCAGTACGGCCTCCCATAACTTTTCTTTGTTCAATGCATCACGAATGGGTGGGGAACACTTGTACCGAGTATCTCTGTCTGGTATCTCTTCAGATGAAAATGTTAGGTAATGAGGGGCTGTTTCGACGCTTATGCTGTCCCCACGACGTTTTGCTTCCTTAATAAGGTCTAAGGAAGCACTTGAATCAGACAAGTGAACAATGTGAACATGTGCTCCTTCTAAAGTACCGCCAATTCTAGTATCCTTTGTAACATCTACAAGTTCTTTAATGGCTGCCTCTTCCCATGAAGGTGGTCTAGTGACGAGATATGTATTGTAAGCGTGCGGGTCACCGTTACCTTTAAGTTCCAAATTACTTTTAGAATCTTGTTGAATCTCGGAATGCACAACTAAAGGTCTTTTATATTTTGCCAACACAGACAATCCCTCCTTGATATGTTCAATAGTGGTCATCGGAAAGTCATTGATTCCTGAAGGGCACATAAAAGACTTTACACCAAGAACACCAGCATCTAAGAGACCTTCAAGAATACTGGTATTTTGTGCATTTTCAGGGATCAAGCCTCCCCAAAAACCAACATCAACATAGATTTTATTCTCTGCAGCTTCAAGCTTCAGTTCCAGTGTTTCCTTAGACACGGTTGTTGGGTGATTGTTTAGAGGCATGTCAACCACTGTAGTTACACCTCCAGCAGCAGCAGCTCTGGTACCAGTGACAAATCCCTCCCATTCGGTTCTGCCGGGCTCATCAAGATGTACATGCACATCAATCAAGCCAGGCATGATAAAAGCATCTCCATAATCAATCACTTGTTGAACAGAACTCCCCTGCTTGCCATATCCTTCCACAATAGATGCTTATTTTTCCCTTCTTATTTTATCTCCACTGATCCAAGAAATGATTCCTTGTAGAAACG
TARGET=1460,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3669
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.329550
PRIMER_LEFT_SEQUENCE=TGTTTAGAGGCATGTCAACCAC
PRIMER_RIGHT_SEQUENCE=TCATTTCTTGGATCAGTGGAGA
PRIMER_LEFT=1423,22
PRIMER_RIGHT=1671,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.670
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.5127
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.307443
PRIMER_LEFT_1_SEQUENCE=GCTTCAGTTCCAGTGTTTCCTT
PRIMER_RIGHT_1_SEQUENCE=ATGGAGATGCTTTTATCATGCC
PRIMER_LEFT_1=1381,22
PRIMER_RIGHT_1=1569,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=60.307
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=0.5127
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.307443
PRIMER_LEFT_2_SEQUENCE=AAGCTTCAGTTCCAGTGTTTCC
PRIMER_RIGHT_2_SEQUENCE=ATGGAGATGCTTTTATCATGCC
PRIMER_LEFT_2=1379,22
PRIMER_RIGHT_2=1569,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=60.307
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.5348
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.329550
PRIMER_LEFT_3_SEQUENCE=GCTTCAGTTCCAGTGTTTCCTT
PRIMER_RIGHT_3_SEQUENCE=TCATTTCTTGGATCAGTGGAGA
PRIMER_LEFT_3=1381,22
PRIMER_RIGHT_3=1671,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=59.670
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.5786
PRIMER_LEFT_4_PENALTY=0.249074
PRIMER_RIGHT_4_PENALTY=0.329550
PRIMER_LEFT_4_SEQUENCE=GGGTGATTGTTTAGAGGCATGT
PRIMER_RIGHT_4_SEQUENCE=TCATTTCTTGGATCAGTGGAGA
PRIMER_LEFT_4=1416,22
PRIMER_RIGHT_4=1671,22
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=59.670
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_03420 Ps_contig_mira-and-tgicl-ass_franssen_29929
SEQUENCE=TTTTAATTTTTANYTAGCTTAATTATTTCTTTCTTTTGGCATATGCTGATAAGAATTGCAATGTTCAAGAACTAGTTTATGAATTCAACTTCAGAGCACAAATTAAAAGCAGATTACAGTGAGATATTCAAGCTCATGCTATCAATACAAGCATAACATCAAAGAGATCATAAACAGGAACTTCGTTCTGATTTTAAGTGCTATGAAATTCTTAGCTCTAACTATCACAAGAGCAGAGCTTAATAACACTAAGGATTCACCCCTCATATACATTGCTCTCAGCATTTGAAGTCCATAGCGAGAAAACAAGAGTCTATGCTCAACAGTTTATGTATTTAAATATGGATAGTAATCATGAGGCTTGTGCATTATTAGCATTTGGCTCGGGGTCATTTTCTGATGATTTAGCGTCTTCAAAAGTTTCCATTTCAATGGTCACATCATCACTCGTATCAGCCTCAACCATTACTTCGTCGTTGTCTGTTACCAAAGGCTCAGTACCATTTTGCACGCCATCAGATTGCTGCTCCTGCTGTTTTTCACTTGGCTTCTCTATGGCATCAGTCTCTTGTACTGGTTCTTGTGGATATGGATCAGTGCAGATTGTCAATGTAGATGTAACTAACAAATTCTTTTCAAGAGCTAAAGCAAGCTTGGAAAGATTTGGATAAATGTTTTTTGCGTCCAATAGAATCTCACAAACCCTTTGCAATGTAAATGGTGGGCCATCAATAAAACATGTAAGTGCATCATTCAATTTGTTGACCAAATCAGAGTAGGATTCTCTTAGCGAAGCATATTGCTGCTCACTTGTCATTTTTGCCTCAGGGTACTCGGACAATACCTGCTTCAGCTGAAATGATAGCATACTCTTGAGATTATCCCAGTCATGCCAGAACCTCCCGGAGGATGCAATAGCTTGAAGAATCTGAATAACTTCGTCTCTTGAAACATCGTTTTTTTCTTCGGTGACTCTGYGATTTGAAACATTAGCCGCCACATCAGCCACCGCGTCGTTGGGTGCACGGTGGAGGGTGCGTGGATGGTGGTCACCGACAACGATGGTGGGAAGAGGGAGATGATGAAGAGGTTGGGTTTTTGGTGGATGCGGCTGAGATGTTGAGGGAAGTGTTGTCTTCGGTTNNAGGAGAGAGTATTGTATAGTGAGGTTGAGAGAGGTGAGAGTGAGTAGGTCTTTCGTGTGACCGTTGGGGAGTCGGGACTGGTGGTGGAGGTGATGAATATTTGTATGGTTTCTTTGGTGGTGGTGGTGAGTGGTAGATTGGGTG
TARGET=1260,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03421 Ps_contig_mira-and-tgicl-ass_franssen_29932
SEQUENCE=AAGTRGGGAATACAAGGTTGTAATCACCTGTCTTTTCTTCTCTTCTCGTCAATCCTAATTTTTAATTTTTAATTTTTTAATTTTTCCAAAAAATTAGCACCGTTATTTTCTCTCTAAAATCTCCATGTTCCTCTACTCTTGAACTTTGGAGGAGATCCACTTCTCTCTCTCACACCATCTTTCAATTTTCACACAGAAAAAAACAACACCATTTTCGTTAAAATTCTTTCGTTTTCATGGCTGCTCCACCGGCAAGGGCTCGTGCCGATTACGATTACCTCATTAAGCTTCTCTTGATCGGCGATAGCGGTGTTGGAAAGAGTTGTCTTCTTTTGCGGTTTTCTGATGGGTCTTTCACAACCAGTTTCATCACGACTATAGGCATTGATTTTAAGATAAGAACCATCGAGCTTGATGCCAAACGGATCAAGCTCCAAATCTGGGATACTGCAGGGCAGGAGCGGTTTCGGACAATTACAACCGCTTACTATCGTGGTGCTATGGGGATATTGCTAGTCTATGATGTTACAGATGAAGCATCGTTTAACAATATCAGGAATTGGATTCGCAACATTGAGCAACATGCTTCCGATAATGTTAACAAAATACTGGTCGGGAACAAAGCAGACATGGACGAAAGCAAAAGGGCTGTGCCAACGTCCAAAGGCCAAGCACTAGCTGATGAATATGGAATCAAATTCTTCGAAACTAGTGCAAAGACAAATCTAAATGTGGAGGAAGTTTTCTTTTCCATAGCAAGAGACATAAAACAAAGGCTTGCTGACACCGACAACAAGGCAGAGCCGACAACAATCAAAATCAACCAAGACTCTGCAGCAGGAGCGGGACAGGCTGCAACAAAAATCAGCTTGTTGTGGTTGAAAACACATTTTACAAGTTGGTGGTGGGGAACATTATTGAAGAAGAAATTACTTGTATGATAATCATGATGCTGACTTGTGGTTAACGATTCCATTGTGTCATTGTTTATTAATTATGTGCTTGATAAGTAGTTTTGGACTTATATATATTGAATCAAAAAACTGAATGTGATTGTGGTGAATCAATGTCTAACTAACTACCTCTGTTGTNAANNTNAGTATTTTGTAGTCTTTTGTCAAAGTTCNCACTAATTTAAAACCAAAATTTAAA
TARGET=162,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03422 Ps_contig_mira-and-tgicl-ass_franssen_29936
SEQUENCE=TAGGTTGGTAGCCAATGTTTAATATTATTGAACTATGGAAGGAAAATTGTATATGCGAATATGTCTACAATACATTTGTACAATTGGTCTTATTTATAGTTTCCTAAAAATAAATCTTTTCTCAACCTGAGCTTAGAAATACTTTAAAAAAAAAATTAAGTGGATAAGAGAAGATAGAACAAAACAAAAATTAGAGGAAAAAACAGAAAAATGAAATTACATTAATACTATGGCACACATCTTCAATACAAATTTATGAAATTTTTCTTAACTGGTGTGACTCTCTCTCTCTCTCTTGAACTTCTTTTTATCAATGGCTTGTTGCTAGAGCAGTAACCAACAGACCAAGAACCAAAAACGGTTGTGCACTAGCCTGATATTTAACATCGTATTTCACAGGGTCCTTGAGGAAATACTTGAACTGAAATATGACTTGTGGAGCTATTAAGGCAAGTAGAGCCAATGCGTAATACGGTTTACCAGCCCCGAGTAGATATCCTGCGACAGATAATTGTGTAATGTCGATAGCACCAACACATATCCACTTTGCAGTTTCCTCGCCAAAGGCGACGGGAAGAGACTGAAGTCCTAGAGCTCTATCTCCTTCAACACTTTTGAAATCGTTTACGATGGCAATTCCCAAGCCAGCTATGCTGTAAAGGAGTGTGAGAACAATAATGTCTGGTGTAAGAGTCCCAAACAAAGCTTGACCAGCCCACCATGGCAAACTAATGTAACTTGCTCCAAGGGCAAAGTTTCCGATCCATCCATTTTGTTTTAACTTTAAAGGAGGTGCAGAATATATATACGACAGCAGGGATCCACCCAATGCAAGGTAAAAAAGTGTAGGGGAATCATGCCCTGCCCATATATCCAATATACCCGCCGAAGTAAGACCTCCTAGTAGAAGCACCCATATTTGAGTGATTACCTCGTTCTCAGATATCACACCAGAAGGAATCGGTCTATAAGGTTCATTTATTGCATCGATTTCTCGGTCATACCAATCATTCAGAGTCTGTGTATATCCTGTCAGGAATGGACCAGACATCATCATACACAAAATTGATTTAGCAACATCCTCAAAGTCCCAATGGAAATTTCCAGAAGCAGCAGCACCACAAACTACACCCCAAACTAATGGAGGCCAAGTTACAGGCTTTGTAAGTTGAAGACGAATCTTCCATATATTTGATTCTTGAGAAGCTCCTTTAATACCAAGAAGCTGATTGAAGCTTGATCCATTTTTAGCAGGCGCCGTATCCGGTGCCTCAGGTTTAACTTCATTTGCATCGGTTTCAGCTGCTCTAACGGTTAATCTTCTCTTAGGAAATGAGAGAGAAAATGGAGCAAAACTTGGTCGTGTTGGAAGTGAAGAGGTTCTTGTTGTTGTTGTTGTTGTGGGTGTTAATATTCTTGGTGAAACTGAAACCATGTTAATAAGTGAAGCCATTGAAGACAACGAGCTTCGAGATGCCACAAACTGAAGAAGCTAGTGTTATCGATGTTGTTGGTAGCCGTGATC
TARGET=281,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03423 Ps_contig_mira-and-tgicl-ass_franssen_29936
SEQUENCE=TAGGTTGGTAGCCAATGTTTAATATTATTGAACTATGGAAGGAAAATTGTATATGCGAATATGTCTACAATACATTTGTACAATTGGTCTTATTTATAGTTTCCTAAAAATAAATCTTTTCTCAACCTGAGCTTAGAAATACTTTAAAAAAAAAATTAAGTGGATAAGAGAAGATAGAACAAAACAAAAATTAGAGGAAAAAACAGAAAAATGAAATTACATTAATACTATGGCACACATCTTCAATACAAATTTATGAAATTTTTCTTAACTGGTGTGACTCTCTCTCTCTCTCTTGAACTTCTTTTTATCAATGGCTTGTTGCTAGAGCAGTAACCAACAGACCAAGAACCAAAAACGGTTGTGCACTAGCCTGATATTTAACATCGTATTTCACAGGGTCCTTGAGGAAATACTTGAACTGAAATATGACTTGTGGAGCTATTAAGGCAAGTAGAGCCAATGCGTAATACGGTTTACCAGCCCCGAGTAGATATCCTGCGACAGATAATTGTGTAATGTCGATAGCACCAACACATATCCACTTTGCAGTTTCCTCGCCAAAGGCGACGGGAAGAGACTGAAGTCCTAGAGCTCTATCTCCTTCAACACTTTTGAAATCGTTTACGATGGCAATTCCCAAGCCAGCTATGCTGTAAAGGAGTGTGAGAACAATAATGTCTGGTGTAAGAGTCCCAAACAAAGCTTGACCAGCCCACCATGGCAAACTAATGTAACTTGCTCCAAGGGCAAAGTTTCCGATCCATCCATTTTGTTTTAACTTTAAAGGAGGTGCAGAATATATATACGACAGCAGGGATCCACCCAATGCAAGGTAAAAAAGTGTAGGGGAATCATGCCCTGCCCATATATCCAATATACCCGCCGAAGTAAGACCTCCTAGTAGAAGCACCCATATTTGAGTGATTACCTCGTTCTCAGATATCACACCAGAAGGAATCGGTCTATAAGGTTCATTTATTGCATCGATTTCTCGGTCATACCAATCATTCAGAGTCTGTGTATATCCTGTCAGGAATGGACCAGACATCATCATACACAAAATTGATTTAGCAACATCCTCAAAGTCCCAATGGAAATTTCCAGAAGCAGCAGCACCACAAACTACACCCCAAACTAATGGAGGCCAAGTTACAGGCTTTGTAAGTTGAAGACGAATCTTCCATATATTTGATTCTTGAGAAGCTCCTTTAATACCAAGAAGCTGATTGAAGCTTGATCCATTTTTAGCAGGCGCCGTATCCGGTGCCTCAGGTTTAACTTCATTTGCATCGGTTTCAGCTGCTCTAACGGTTAATCTTCTCTTAGGAAATGAGAGAGAAAATGGAGCAAAACTTGGTCGTGTTGGAAGTGAAGAGGTTCTTGTTGTTGTTGTTGTTGTGGGTGTTAATATTCTTGGTGAAACTGAAACCATGTTAATAAGTGAAGCCATTGAAGACAACGAGCTTCGAGATGCCACAAACTGAAGAAGCTAGTGTTATCGATGTTGTTGGTAGCCGTGATC
TARGET=1384,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0230
PRIMER_LEFT_PENALTY=0.014720
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=CTAATGGAGGCCAAGTTACAGG
PRIMER_RIGHT_SEQUENCE=CATGGTTTCAGTTTCACCAAGA
PRIMER_LEFT=1137,22
PRIMER_RIGHT=1435,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=0.2506
PRIMER_LEFT_1_PENALTY=0.242357
PRIMER_RIGHT_1_PENALTY=0.008257
PRIMER_LEFT_1_SEQUENCE=AGCTTGATCCATTTTTAGCAGG
PRIMER_RIGHT_1_SEQUENCE=CATGGTTTCAGTTTCACCAAGA
PRIMER_LEFT_1=1233,22
PRIMER_RIGHT_1=1435,22
PRIMER_LEFT_1_TM=59.758
PRIMER_RIGHT_1_TM=60.008
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.3635
PRIMER_LEFT_2_PENALTY=0.056850
PRIMER_RIGHT_2_PENALTY=0.306698
PRIMER_LEFT_2_SEQUENCE=AAAACTTGGTCGTGTTGGAAGT
PRIMER_RIGHT_2_SEQUENCE=CTAGCTTCTTCAGTTTGTGGCA
PRIMER_LEFT_2=1351,22
PRIMER_RIGHT_2=1495,22
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=59.693
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=0.4156
PRIMER_LEFT_3_PENALTY=0.108929
PRIMER_RIGHT_3_PENALTY=0.306698
PRIMER_LEFT_3_SEQUENCE=GAGAAAATGGAGCAAAACTTGG
PRIMER_RIGHT_3_SEQUENCE=CTAGCTTCTTCAGTTTGTGGCA
PRIMER_LEFT_3=1338,22
PRIMER_RIGHT_3=1495,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.693
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=0.4821
PRIMER_LEFT_4_PENALTY=0.473868
PRIMER_RIGHT_4_PENALTY=0.008257
PRIMER_LEFT_4_SEQUENCE=GGTGCCTCAGGTTTAACTTCAT
PRIMER_RIGHT_4_SEQUENCE=CATGGTTTCAGTTTCACCAAGA
PRIMER_LEFT_4=1265,22
PRIMER_RIGHT_4=1435,22
PRIMER_LEFT_4_TM=59.526
PRIMER_RIGHT_4_TM=60.008
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_03424 Ps_contig_mira-and-tgicl-ass_franssen_29940
SEQUENCE=GTCGGGGAGGTCGACTCCACTCTACCCNGATCTTATGCAAAAGCGTTCTCCAACTTGAAATATTTCTCACTCACTCGGTCGTTCATTGAAAACAGCACTGCAGCGGCGGCAAACACCACTCACTCACTCTATCACTTTTCTCGATTCCCTTTTCCTCATCCACAACTCATTCGTCATTCTCTTCCGAGATCATTGAAGCATACAGCTGTAGCAACATGTGGTCAAGCATTAATTAAGCAAAGAAAAACTACTTAAAAATGCTGTCTACTAGTGTTGCTGCTCAGCTTCCCAGTTGCTGGTACCGCATTCCCAGCCATCTTAATGTCCATCATCATACCCGACAAAAGAGAAAACTCACCACTCTATCACTTGCCAATGCAGATAACCAAATCCCTCTCAGAAACTTCTACAGTCAAAGTCTTCCTCAGACATACAGTTGCTGCAAAGGGATAGAAAGCAATTACAGGTCTAGATACAATTCCAATCCAAATCAGGACGACAGACACCTATTCCTTATTAAATTCTTCTCACTTGCTGACTCAGAAGACACCAATAATCAAAATTCAGAAACCAGAAACCACAACCAACATCAAGTAGACAGACAAAGGGAATTCAAGGAAAACGACAAAAAAAGTAGCACTTTTCACTAAGATGTGGTGGGTAGATTTGAAAGCGGCATTTGGCCAAAGCATCAACTTTGAGGGCATTCTTTGTTCAACTATGGTGATCTTAAAAGACCCACATTTGGCACTGCCCCATATTTCAGTTCGTGATATACGATACATTGACTGGGCTGCTCTACGTAGAAAGGGATTTAAGGGCGTTGTCTTTGACAAAGATAATACTATTACAGCGCCTTACTCTTTAACGCCCTGGCCTCTGCTCGAGTCTTCATTGGAAAGTTGTAAATCAGAATTTGGTCCAGATATTGCTGTATTTAGTAACTCAGCTGGACTTCGTGAGTATGACCATGATGGTTCAAAAGCTAGGAATCTTGAGAGTGCAATTGGAATTAAAGTCATCAGACACAGAGTGAAGAAGCCAGGTGGCACAGCTGAAGAAGTTGAAAAACATTTTGGCTGTGATTCATCACAGTTAATCATGGTGGGCGACCGTCCCTTCACTGACATAGTTTATGGCAATCGAAATGGATTTCTTACTATCTTGACAGAGCCATTGAGTCTTACGGAGGAGCCATTCATCGTTAAGCAGGTGAGGAAGCTAGAAACTACATTTGTAAATTACTGGTCTAGAAAAGGATTGAAGCCACTTGAGCAGAAGCTATTGCCTGATCCCAGACATTGTGTCAAAGAGCCACGTCCTTGATAAACTTGTCTCCAAAAGACACTAATTTTTTAGGACATGAAAATGCTCCCCTTTAGTAATGCATACAAGTTAATTTTCGTTGAANNTATTTTTAGTTTTTTTTTCCTTTTAACAAAAACCGGTATGACTAAGTTAAGTCATCTAACTAAA
TARGET=117,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1328
PRIMER_LEFT_PENALTY=0.084327
PRIMER_RIGHT_PENALTY=0.048460
PRIMER_LEFT_SEQUENCE=CCACTCTACCCNGATCTTATGC
PRIMER_RIGHT_SEQUENCE=GGAAAAGGGAATCGAGAAAAGT
PRIMER_LEFT=16,22
PRIMER_RIGHT=154,22
PRIMER_LEFT_TM=59.916
PRIMER_RIGHT_TM=59.952
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=139
PRIMER_PAIR_PENALTY_1=0.1536
PRIMER_LEFT_1_PENALTY=0.084327
PRIMER_RIGHT_1_PENALTY=0.069319
PRIMER_LEFT_1_SEQUENCE=CCACTCTACCCNGATCTTATGC
PRIMER_RIGHT_1_SEQUENCE=AGGAAAAGGGAATCGAGAAAAG
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=155,22
PRIMER_LEFT_1_TM=59.916
PRIMER_RIGHT_1_TM=60.069
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=140
PRIMER_PAIR_PENALTY_2=0.2252
PRIMER_LEFT_2_PENALTY=0.084327
PRIMER_RIGHT_2_PENALTY=0.140832
PRIMER_LEFT_2_SEQUENCE=CCACTCTACCCNGATCTTATGC
PRIMER_RIGHT_2_SEQUENCE=AATGACGAATGAGTTGTGGATG
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=177,22
PRIMER_LEFT_2_TM=59.916
PRIMER_RIGHT_2_TM=59.859
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=0.2853
PRIMER_LEFT_3_PENALTY=0.236882
PRIMER_RIGHT_3_PENALTY=0.048460
PRIMER_LEFT_3_SEQUENCE=ATCTTATGCAAAAGCGTTCTCC
PRIMER_RIGHT_3_SEQUENCE=GGAAAAGGGAATCGAGAAAAGT
PRIMER_LEFT_3=29,22
PRIMER_RIGHT_3=154,22
PRIMER_LEFT_3_TM=59.763
PRIMER_RIGHT_3_TM=59.952
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.3011
PRIMER_LEFT_4_PENALTY=0.174518
PRIMER_RIGHT_4_PENALTY=0.126537
PRIMER_LEFT_4_SEQUENCE=ATTTCTCACTCACTCGGTCGTT
PRIMER_RIGHT_4_SEQUENCE=GCTGTATGCTTCAATGATCTCG
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=205,22
PRIMER_LEFT_4_TM=60.175
PRIMER_RIGHT_4_TM=59.873
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_03425 Ps_contig_mira-and-tgicl-ass_franssen_29946
SEQUENCE=TCGGGGGTCGGTCTTTGTACTTAATATAGAAGAAGGAGAAGACTGTAGGGGTGACCGATTTCCCCAATTCGAGGCAAACCCTCGTTCCGAAATCGCGACACCGCGCTTATCTCATGGCGGCCTCTTCTTCCTCTCTCTCTTCCGATTCTTCCTCTCACCGTCGCAGGCGTCACCACCGTAATCGCCGAGACCGCGACAAGGACTCCCTTAAGATCCGAAAAAAAACCAAGTCTCACTCCCACACCAAACGACGTCGCAAGCATCATCGCTACTCTTCCGATTCTGATTCATATTCTTCATCCCCCCTCTCTGATTACTCCAGAAGTGAGAGTTCTTCTGATAGTGAGCATGAAACATCTCATCGTACAAAGAAGCACAAAAAGAGTGACAAACCTAAGAAGAATAAGGAAAAGGAGCGAAGTAAAAGTCATCGCCATAAGAGACAGAAGCACAACCTAAAAGAGAAGCAGAGTGGTGAAAGGAGCAGCAGCCCTGTTCAGCTTTCTAAAGTTTTTAGGGCGCAACAAAGATGATGGTGTCCGTCGCAGTGCTGTGT
TARGET=131,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8726
PRIMER_LEFT_PENALTY=0.749986
PRIMER_RIGHT_PENALTY=0.122569
PRIMER_LEFT_SEQUENCE=GAAGGAGAAGACTGTAGGGGTG
PRIMER_RIGHT_SEQUENCE=AATCAGAATCGGAAGAGTAGCG
PRIMER_LEFT=31,22
PRIMER_RIGHT=287,22
PRIMER_LEFT_TM=59.250
PRIMER_RIGHT_TM=59.877
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.8726
PRIMER_LEFT_1_PENALTY=0.749986
PRIMER_RIGHT_1_PENALTY=0.122569
PRIMER_LEFT_1_SEQUENCE=AAGGAGAAGACTGTAGGGGTGA
PRIMER_RIGHT_1_SEQUENCE=AATCAGAATCGGAAGAGTAGCG
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=287,22
PRIMER_LEFT_1_TM=59.250
PRIMER_RIGHT_1_TM=59.877
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=1.0351
PRIMER_LEFT_2_PENALTY=0.749986
PRIMER_RIGHT_2_PENALTY=0.285163
PRIMER_LEFT_2_SEQUENCE=GAAGGAGAAGACTGTAGGGGTG
PRIMER_RIGHT_2_SEQUENCE=GAATCGGAAGAGTAGCGATGAT
PRIMER_LEFT_2=31,22
PRIMER_RIGHT_2=282,22
PRIMER_LEFT_2_TM=59.250
PRIMER_RIGHT_2_TM=59.715
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=1.0351
PRIMER_LEFT_3_PENALTY=0.749986
PRIMER_RIGHT_3_PENALTY=0.285163
PRIMER_LEFT_3_SEQUENCE=AAGGAGAAGACTGTAGGGGTGA
PRIMER_RIGHT_3_SEQUENCE=GAATCGGAAGAGTAGCGATGAT
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=282,22
PRIMER_LEFT_3_TM=59.250
PRIMER_RIGHT_3_TM=59.715
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=1.0428
PRIMER_LEFT_4_PENALTY=0.749986
PRIMER_RIGHT_4_PENALTY=0.292834
PRIMER_LEFT_4_SEQUENCE=GAAGGAGAAGACTGTAGGGGTG
PRIMER_RIGHT_4_SEQUENCE=TTCTGGAGTAATCAGAGAGGGG
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=323,22
PRIMER_LEFT_4_TM=59.250
PRIMER_RIGHT_4_TM=59.707
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_03426 Ps_contig_mira-and-tgicl-ass_franssen_29951
SEQUENCE=CTTCATTATGGANCATCCTTACTAACTTAGTTCATTAAATTAGTATCAACTCTCTGCATACAACATTTGCACTCAAAAATAAGATAGTCTTAAATTGGAATGTAGATTCATATTTATCAGACAATATTTTTTCTTCCAGAAAATATTAAATTACATGGGTCTAAGAGCTTATGGCTACAAAATGAATAATTATGAAATCTTCAACAGAATCCTGCCAGATAGAACTCTATCATGTCAACAGCATTTTATTTGCTTCCAATTATTTCATTAACTGTTTCCTTTACTTTCTGTACAAGTTCTTCCCTTTCTGGTTTGAAAACTAGGTTCTTGTATGCAAACCACCCGGAATAGCCAATGCCTATTAATTCAAGAACTCCAGGAATCAAGGGAAGCTTATCTATTGCTGAAATCAAACCAGTTGAACTCCATAGTGCAATCACACTAGCTACAATCAATGTACTCACTGCATATTTATCATCCACTTTGTCCCAAGTTTCTTGAATGGACTTAACAAACTCAGGTAGTTCAGTTGAGTCAACTTCTGCTTGTGCATCTCCAGTGGCCATAACCATAACATCACGACCAATCTTTCGATAATGAGAATTTGTCTTCACTGCATGATTCTGAGACAACAAAGGGAGTGATGATGGAAGAGTGGTTAGTCTCATACATTGTGGAGAAGAAGCTTTGCAATCAAAAAGGTTAGAGGAAGAGGTTATAGTGAGAGAAGAAGAAGAGATTAAGGCCATTGTTATTAATTAATGAACACTTTTTGAAGGTGTATAATTTTTATTTTCCTATGAAAAATTGAGTCCACTAAGTTATTGATATGTTTGAATTGTGTTTGATAATTTTGCAAGTGATTGTGGAGATCCTGTTGCTAGAAATTGAAGTGAAGTAGAACCACTTATTTATTTTTCATTTGGATAATGTAGTGATATTATTATTTTGATAGATC
TARGET=726,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2406
PRIMER_LEFT_PENALTY=0.115965
PRIMER_RIGHT_PENALTY=0.124655
PRIMER_LEFT_SEQUENCE=TCTGAGACAACAAAGGGAGTGA
PRIMER_RIGHT_SEQUENCE=TCTAGCAACAGGATCTCCACAA
PRIMER_LEFT=622,22
PRIMER_RIGHT=884,22
PRIMER_LEFT_TM=59.884
PRIMER_RIGHT_TM=59.875
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.2406
PRIMER_LEFT_1_PENALTY=0.115965
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=TCTGAGACAACAAAGGGAGTGA
PRIMER_RIGHT_1_SEQUENCE=TTCTAGCAACAGGATCTCCACA
PRIMER_LEFT_1=622,22
PRIMER_RIGHT_1=885,22
PRIMER_LEFT_1_TM=59.884
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.2732
PRIMER_LEFT_2_PENALTY=0.148509
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=TGAGACAACAAAGGGAGTGATG
PRIMER_RIGHT_2_SEQUENCE=TCTAGCAACAGGATCTCCACAA
PRIMER_LEFT_2=624,22
PRIMER_RIGHT_2=884,22
PRIMER_LEFT_2_TM=60.149
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.2732
PRIMER_LEFT_3_PENALTY=0.148509
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=TGAGACAACAAAGGGAGTGATG
PRIMER_RIGHT_3_SEQUENCE=TTCTAGCAACAGGATCTCCACA
PRIMER_LEFT_3=624,22
PRIMER_RIGHT_3=885,22
PRIMER_LEFT_3_TM=60.149
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.4135
PRIMER_LEFT_4_PENALTY=0.288804
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=TGATTCTGAGACAACAAAGGGA
PRIMER_RIGHT_4_SEQUENCE=TCTAGCAACAGGATCTCCACAA
PRIMER_LEFT_4=618,22
PRIMER_RIGHT_4=884,22
PRIMER_LEFT_4_TM=59.711
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_03427 Ps_contig_mira-and-tgicl-ass_franssen_29966
SEQUENCE=TAATCTCACTAACTAATCAAAACCTCTGTAAAACTGTTTCAGGTTCAACCCCACATATTTCCTAGATTGCTCCATTCAAAATTTTATTCACTTCGTGTTCTACCGTTCTGGCCAATTCAACGGCTGTTCCAACGTAAGTATGCGGCGTTAACTTTAAGAGATAGGTCTTTGCATTCTGTGGAATATCTAAGCCTTCAATGAAGTCTCTTAAACTTTCTTTGGTAATTGCTTTCCCTCTTGTCAGCTCTTTTAACTTCTCATAAGGCTCAGGAACACTGTATCTTCGCATAACCGTTTGTATTGGTTCAGCAAGTATCTCCCAGCATTGGTTCAAGTCTTCGCTCAAGCGAGCTTCGTTGACCTGAAGCTTCCCAATTCCTTGAAGTGTGCTTTTATATGCAAGAAGAGAATGACCAAAATTGACACCCATGGTCCTTAAGACAGTTGAATCAGTCAAATCCCTCTGCCAACGTGAAATCGGCAACTTCATGCTTAGATGAGAAAAACCTCCATTAGCCACGCCTAAATTGCCTTCGCTATTCTCAAAATCAATGGGATTCACTTTGTGAGGCATAGTTGATGACCCAATCTCCCCGGCCTTAGTTGTCTGCTTAAAGTAGCCCAAAGATATATAGCCCCATACATCTCTATCAAAGTCGATTAATATATTGTTGAACTGGATGAATGAATGAAAAATCTTTGCCATGTAGTCATGAGTTTCAATCTGGGCAACATAAGGATTAAAACATAATCCAAGAGAAAGTACAAACTCTTGTGCAATGTCAGGCCACTTAACATCARGGTACGCAGRAAAATGCGCGTTGTAATTTCCAACCGCACCGGCAAATTTCCCCAAAACCTCAACTTGAGACAGTTCCTTCCTTTCTCTGCTTAGTCTCACAGCAAATATCGCCATTTCCTTCCCTAAAGTTGTTGGTGAAGCTGGCTGTCCATGAGTGCGAGAGAGCATAGAGATATGAGCATTGTCTTTGGCCATTGTACACAAAGCTTTGATTATTTTATTCATGGCAGGAAACATAACAGAATCCATTGCTTCTTTCAACATCAAGGCATGAGCAAGATTATTTATATCCTCAGACGTGCAAGCAAAGTGAAAAAATTCAAGCACCTTAGCAATTTCAGCATTTGATTGGCACTTGTGTTTCAAGAAATACTCTACAGCTTTAACATCGTGATTTGTAATCTTCTCAAATTTGTTATGCAAGCGGCACGTGTTATTCAACCTTCAACGATGCAATTGATTCTATCCAGCCTTCCATCAACAATCTACCAATATTCAACAGTCGACGTCGTTTGCAGTTGGTTTCAGTCCAAGAACCGAAAAATCAAACTTTCCAATATGTCAAGGATGAGGTAAGTTTCTACTGGTTTGTAATTTCCCAAGTTTCTACTGGTTTATTCTTTGGCAGGTAGGCTTCACTTTGTTGCATCAAACCGCTGCTAAGGACTCATTGCAGACCGCTGATCAATGTCATATAGAGACAGCAGAATTTCGGCACCAATCAAAGTAATAATGAGCCACTCTAGGAAATCCGGTTAACGAGAGTTGATCTTGCTGTATGTTTCAGGACGAACACCGGGTCTTTTGTCAACCAAAGAGCCATCAACCTTTTGAGCTACTAATTGCCATGAAACAATTGCACTGTAATGTTCAACAACGTGAAGTCATTGGAGATTCCAAAAGCAATTTTCGGTTTTTTTTTTATCACAACTCAGGTTTTACTCGGTTGGCAAAGAAACCTGGAGCAGATTCAACTCCCAATGCCTCTGCACCACTAAGCCACATCGCCTCACTCATAAACCACTTGCGAGAGGGTTTTTGCTTACGTTTTTTAGACATACGGTTCTTAGTCACATGCCAATTCATGTGACTGCTGTGCTCATCTTGGGATTTAAAACGGAGGCCACAAGTTGTGCATTGATGGTGGAGGGAAAAGGCTGAAGAGTTTTTTCTGTTGTGGAACTCAGATAGACTTCTTTCATTTGAATTGCTCATGATGTATGCCATGAAGTTTGATGTATGTAGCATAAAATGTATATGAATAATCAATGAGGCATAATGTAATGCTTATGTAAAATGCTTGTTTTAATATGTAAAATGTGCTCATGATATATAAATATTAATGGAATGAAGTGTATGAGAATGTAGCATTAACGTANNAYNCTTTTTCT
TARGET=681,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03428 Ps_contig_mira-and-tgicl-ass_franssen_29968
SEQUENCE=AGTTTTAAAATACTGGCGAAGTTGTATTCTCATATTAAGAGTCCTTAGAATCCGTGAAAGATTCGACAACACATTATTCTCAATTTATTATTATTTGTCACACAGAACTAATCTCTAATCTCTCTTATTATTATTAAAAAAGATTCCATTTTCAATCACTTTACCTCTTCTGGACCCACGGTACAACATTATTCTTATCTTCACAATCGACTTTTTTTAAGTGACCCCATATCCTTAGCAATCTGTCTAAGCACAAACTTCTGAACCTTCCCCGTAGAGGTTTTAGGAAGCTCCTCCTTGAACACCACCGTTTTCGGAACCATATAATGCGGCAAATTCTTCCTACAATACTCAACTATATCTTTCTCCGTCGGAATTCCCTTTCTTTCAACCCATCTTTCAAACTCACAAACGCACACGGCGTTTCCCCCCAAAATTCATCAGCTCTCGCCACCACCGCCGCTTCATTCACCGCCGGATGTCCATACAACACCGACTCAACCTCCACACTACTCAGATTCTCACCGCCACTGATAATCACATCCTTTGACCTATCTTTAATCTCCAAATATCCATCCTCATGCATCACTCCAACATCACCGGTGTAAAACCAACCATTCTTCATAGAACTCGCGGTTCCTTCAGGATCCTTCAAATATCCTAACATAACACAACCACCTCTCATAACAACCTCCCCCAAAGTCGCTCCGTCGCGTTTCACACTTTCACCGGTGGGACTCACCACATCAACGCTAGTCATCCCAATCGTCCTCACTCCTTGCCGTGACTTCAACCGTGCTCTCTCCGTCGCAGGTAACAAATTCCATTTCTTCTTCCACGTGCAACAAACCACCAACCCACCGGTTTCAGTTAATCCATACCCGTGACTCACCGTAAAACCTAACGACTCAGTACGAAAAAGAATCGCAGCTGGCGGCGGTGCTCCGGCAGTTAGAATATGAACTGGTTTCTCCAACGGTTTAATATCCGGCGAGTTAGTTAGCATGTTGAGAATAACCGGAGCACCGCACATGTGAGTCACGTGATGCTTTTTGATCAGAGAGTAAACAATTTCAGTATCGAATTTTCTCACGCAGATGTTAGTTCCACCAACGGCCGCGATCCCCCACGGGAAGCTCCACCCGTTAGCGTGGAACATCGGTAGCGTCCAGAGGTAAACCGGTTGTTTAGGAACTGACCATTCGATTAAAGAATCGAGGGTCACGATGAAAGCTCCTCTGTGGGAATGAACTACACCTTTGGGAGACGACGTCGTTCCGGAAGTGTAGTTGAGAATCATCGGGTCCCACTCGGAAATAGGATGAAGCCAGTTAAAATCTGGATCACCGTTCGTTATTAGGTCATTGTAAGTGGAGAAGAAACCAACGGTGGATGATGAAGAAATAGAAACAGAAACAGGTTCTGGTTCTGTTTCTTCATCGTCTTTGATGAGGATTAGAAGAGGACGTTGTTGTGAAGAAGGGAACAATGACAAAGCTTCGAGTACGAGATCGCGTGAAGCGTAGTCGACGAAAACTAGTTTAGACTCGGAGTGGATGAGGATATTGGAGATTATACGCGCGTCGAGGCGCGTGTTGATGTTGTTAAGGATTGCGCCGGCGAAGGGGACGGCGAAGTGGAGTTCATACATGGCGGGGATGTTGGGAGCGATGACTGAGACAACGTTTCCGCGGCGGATTCCGAGGGAGGTGATTGCGGAAGCGAGTTGGAGACATCGACGGTGGGTTTGGGACCATGTGAAAACGGTGTCGTTGTAGATGATTGAAGGAACATCGTTGTAGATTGTTGATGCTCGTTCTAGAAAGGTGAGTGGTGTGAGAGGGGAGGTGTTTGCGGGGTTAGGTTTTAGTTTTTCCATTGACTTTGYCTTTTTTTTTGTTGCTATNRANTGGGAAAGGAA
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03429 Ps_contig_mira-and-tgicl-ass_franssen_29974
SEQUENCE=AGAGGTGTGATTTGATCTGAGTTGTTAGTTGAGTTTATTGGTGGAAAGGAAAGGAAAGAATGTCATCACTGGGAACTTCAAAGGGGATTCTAGAGATCGCCAAGTTTGGGGTTTACGTTACTGTACCCATCGTTCTTATGTACGTTTATGCCAGCAATACCGACAACACCCTCCACAAGTTCATCGGAAAAAAGTCATACGTTGAATATCCCAAGGATACACAAAAGCCACCACCGCCAGAGGAGCTTAGAGAGATGGCGAGGGAGATAGCTCGCAAAAGGAACAACCCCTGATGCTTCTTGTGGGTTGGCAATAGTAATAGTAAACGTTTGTAATGGACACCGTGTGAACTACGAATATTTGCTTCTTTCAATTGTTTCTGTTGTTTTATTATGAAATTTTCTGCAATGAAAGGAACATATTGTTATTAAGTAAATGAGTCAACGTTTGCAATTTCATTATCACGACGAAAAYCAATTCTTCATTGAC
TARGET=43,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03430 Ps_contig_mira-and-tgicl-ass_franssen_29975
SEQUENCE=CGGGTTGTTTATTTAGGAAATCATCTGTGGTGGTTCACACTATCCCTTATAATAATTGTTCATAGCTGAGATATATATATAGACAATTCTAAAGCGCCGCAAAACGTAATTCATTACGTCAGGTTATTGCAACACCCATATGGTTTGATCTGAATTGAGAGGTGTGATTTGATCTGAGTTTGTTGGTGGAAGGAAAGGAAAGATGTCATCACTGGGAACTTCAAAGGGGATTCTAGAGATCGCTAAGTTTGGGGTTTACGTTACTGTACCCATCGTTCTTATGTACGTCTATGCCAGCAATACCGACAACACTCTCCACAAGTTCATACGGAAAAAGTCAATACGTTAGAATATCCCAAGGATACACAAAGCCACCACCGCCAAGAGGAGCTTAGGAGAGAGTGGCGGAAGG
TARGET=71,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1175
PRIMER_LEFT_PENALTY=0.109200
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=TCTGTGGTGGTTCACACTATCC
PRIMER_RIGHT_SEQUENCE=CCACCAACAAACTCAGATCAAA
PRIMER_LEFT=23,22
PRIMER_RIGHT=188,22
PRIMER_LEFT_TM=59.891
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.1214
PRIMER_LEFT_1_PENALTY=0.109200
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=TCTGTGGTGGTTCACACTATCC
PRIMER_RIGHT_1_SEQUENCE=CTTTCCTTCCACCAACAAACTC
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=196,22
PRIMER_LEFT_1_TM=59.891
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=174
PRIMER_PAIR_PENALTY_2=0.1442
PRIMER_LEFT_2_PENALTY=0.109200
PRIMER_RIGHT_2_PENALTY=0.035021
PRIMER_LEFT_2_SEQUENCE=TCTGTGGTGGTTCACACTATCC
PRIMER_RIGHT_2_SEQUENCE=TGGGTACAGTAACGTAAACCCC
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=271,22
PRIMER_LEFT_2_TM=59.891
PRIMER_RIGHT_2_TM=60.035
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=10.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.1614
PRIMER_LEFT_3_PENALTY=0.153161
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=GTGGTGGTTCACACTATCCCTT
PRIMER_RIGHT_3_SEQUENCE=CCACCAACAAACTCAGATCAAA
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=188,22
PRIMER_LEFT_3_TM=60.153
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=163
PRIMER_PAIR_PENALTY_4=0.1653
PRIMER_LEFT_4_PENALTY=0.153161
PRIMER_RIGHT_4_PENALTY=0.012153
PRIMER_LEFT_4_SEQUENCE=GTGGTGGTTCACACTATCCCTT
PRIMER_RIGHT_4_SEQUENCE=CTTTCCTTCCACCAACAAACTC
PRIMER_LEFT_4=26,22
PRIMER_RIGHT_4=196,22
PRIMER_LEFT_4_TM=60.153
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_03431 Ps_contig_mira-and-tgicl-ass_franssen_29986
SEQUENCE=AGGTGCAAAGCCCGCTGTTAATGAAAATGCTGGCGGAATTATAACTGCAGGACAAGCTAAGAGATCAGCTTCTARGTCCGTGGTGCGTGGCTCTATTATTCAACTGAAGTGCGCAAATCGCTCGCTAGCAGTTTCGGTGATCTCTCTTCCGATTTCCTCTGAATCTAATCATTCCCGATAGATACAAGAATGGCATCCAATAGGGGTCAAGGAGGAATTCAGCAATTGTTGGCTGCTGAACAAGAAGCACAACGGATTGTGAATGCTGCGAAAAATGAAAAATCGGCTAGGTTGAAACAGGCCAAAGAAGAGGCTGAAAAAGAGATTGCTGAATACCGTGCCAAGCTTGAATCTGAGTTTCAAAAGAAAGTTTCGGATAGTAGTGGTGACTCTGGAGCCAATGTTAAGCGACTTGACCAAGAAACTGAAGAAAAAATCCATCATCTGAAAGAAGAAGCTGCAAGAATTTCGGAGGATGTTGTTGCCATGCTCCTTAAGTATGTAACGTCTGTGAAAAAGTAGGAACCTTTGATGATATGTTGGTGCAGCTGAGTGGACCCCTATATTATGTTGATTATCATATCACTAAGTTTATTTTTGGATCCAGATATTAAGGCGTGGAGAACATTATCATTATTTAAAATCTTACTTCGATGTATTAAAATAAATAAATTTCGAGTTTGTTCAGATTTACAGTTCATAATTCAAAGACATGTCTATTTGTGTTGACGTTGTAACAAAATGTATGAGGAGTGAAGACCATATCTTTAGGTGTGTGTCAAGTAGAATGCATTGTTTATTCCTTCCTAAGAGTGTTTGAATGAAATTGTATGGGTATTTTCTAGCAAATNTAAAAGTACTTAAAGTTACAAATAAC
TARGET=662,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03432 Ps_contig_mira-and-tgicl-ass_franssen_29990
SEQUENCE=ACCACACATCCCTAATATTGTATACTATATACACAATTATTTCTGANCGNGYATTAGTTCTGAAAGAGGAAAGGTTTCGTGAACAAGATCACTATCCTACTACTATCCGATTCATTCCTGATAACTCTTGTCTACCTCCATCCTTATCTCCAAACCCCCAAAACACCACCATTCTATATTCATTCATTAGTTATATTATTACTATATCATAAGATTGCCATAGCGATATAAAACAAGAACAACAACAACTACTACTAAAGCTAACTAACCAATCATATCAATAACACACACACACACACGGTTTTATTTATTGTTGTTAAACATGCTTAGAACAAGAATCAACACAGCACGTTCCATTTTTTCATCTATTTTCAGAATCAATTCTTCTTCTTATTCCACTTCCTTCCTCTTTGACGACACACAGATTCAGTTTAAAGAGAGTGTTGCTCAATTTGCTAATGAAAATATTGCCCCTCATGCTTCCAAAATTGACCACACTAACTATTTCCCACAGGAGGTTAACTTGTGGAAAAGTATGGGAGAATTTAATCTCCATGGGATTACTGCACCGGAGGAGTATGGAGGGCTTGGTCTAGGTTACTTGTATCATTGTATAGCAATGGAAGAGATTAGCCGCGCTTCAGGATCCGTTGGTCTTTCTTATGGTGCTCATTCTAACCTATGTATCAATCAACTGGTGAGGAATGGAAGCCATGCTCAGAAACAGAAATACTTGCCAAAGCTTATCTCCGGGGATCACGTGGGAGCTTTGGCGATGAGTGAACCCAATTCTGGGTCTGATGTTGTCAGCATGAAATGCAAGGCTGATCGTGTAGACGGAGGATATGTACTTAATGGTAACAAGATGTGGTGTACTAATGGGACCGTTGCTCAAACATTAGTTGTCTATGCAAAAACAGACATAACAGCAGGGTCAAAAGGAATCACTGCATTCATCATCGAGAAGGGAATGCCTGGATTCAGTACTGCGCAGAAATTGGATAAACTAGGGATGCGAGGAAGTGATACATGTGAGCTTGTCTTCGAGAATTGCTTTGTTCCAGACGAAAATGTTCTTGGGAAAGAAGGAAAAGGAGTCTATGTCATGATGTCGGGGTTGGATTTGGAAAGGCTTGTTTTATCAGGTGGTCCCCTTGGTATTATGCAGGCATGTCTCGATGTTGTCCTTCCTTATACTCGACAACGAGAGCAGTTTGGTCATCCTATTGGAGAGTTTCAGTTCATCCAGGGAAAAGTTGCAGACATGTATACGTCTTTACAGTCCTCAAGGTCTTACGTGTATTCTGTAGCTCGGGACTGTGATAACGGAAAAGTTGACCCGAAGGATTGTGCTGGAGTTATACTCTGTGCAGCTGAAAGAGCAACGCAGGTTGCTTTGCAGGCGATACAATGTTTAGGCGGGAACGGTTATGTGAATGAATATCCAACCGGTCGTTTCCTTAGAGATGCTAAACTCTACGAGATCGGAGCCGGAACTAGTGAGATCAGAAGAATGATTATAGGACGCGAGCTCTTCAAGGAGCAATGACATGCTAGTCCTTTGGATTTTGACTGGAACTGAGCCTCTTGGAGATGATTCTTTGTAAAAGAATTTTTCATTAATCAAATGAGAATTGTGTTAACCAAAATTGATCAACAATACAAGAAACAAAAAACTGCCATGTCATATTTGTATGTGCTCTTTTGCTGTGAAAATCTTGTAAGCAATTCAAACAATATTTTCTAATTTTCTGTTAGAAATTAGTATCTATATTTGATAAATTTTAGAAGACAAACAACATATTTNACTAACNG
TARGET=238,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03433 Ps_contig_mira-and-tgicl-ass_franssen_29990
SEQUENCE=ACCACACATCCCTAATATTGTATACTATATACACAATTATTTCTGANCGNGYATTAGTTCTGAAAGAGGAAAGGTTTCGTGAACAAGATCACTATCCTACTACTATCCGATTCATTCCTGATAACTCTTGTCTACCTCCATCCTTATCTCCAAACCCCCAAAACACCACCATTCTATATTCATTCATTAGTTATATTATTACTATATCATAAGATTGCCATAGCGATATAAAACAAGAACAACAACAACTACTACTAAAGCTAACTAACCAATCATATCAATAACACACACACACACACGGTTTTATTTATTGTTGTTAAACATGCTTAGAACAAGAATCAACACAGCACGTTCCATTTTTTCATCTATTTTCAGAATCAATTCTTCTTCTTATTCCACTTCCTTCCTCTTTGACGACACACAGATTCAGTTTAAAGAGAGTGTTGCTCAATTTGCTAATGAAAATATTGCCCCTCATGCTTCCAAAATTGACCACACTAACTATTTCCCACAGGAGGTTAACTTGTGGAAAAGTATGGGAGAATTTAATCTCCATGGGATTACTGCACCGGAGGAGTATGGAGGGCTTGGTCTAGGTTACTTGTATCATTGTATAGCAATGGAAGAGATTAGCCGCGCTTCAGGATCCGTTGGTCTTTCTTATGGTGCTCATTCTAACCTATGTATCAATCAACTGGTGAGGAATGGAAGCCATGCTCAGAAACAGAAATACTTGCCAAAGCTTATCTCCGGGGATCACGTGGGAGCTTTGGCGATGAGTGAACCCAATTCTGGGTCTGATGTTGTCAGCATGAAATGCAAGGCTGATCGTGTAGACGGAGGATATGTACTTAATGGTAACAAGATGTGGTGTACTAATGGGACCGTTGCTCAAACATTAGTTGTCTATGCAAAAACAGACATAACAGCAGGGTCAAAAGGAATCACTGCATTCATCATCGAGAAGGGAATGCCTGGATTCAGTACTGCGCAGAAATTGGATAAACTAGGGATGCGAGGAAGTGATACATGTGAGCTTGTCTTCGAGAATTGCTTTGTTCCAGACGAAAATGTTCTTGGGAAAGAAGGAAAAGGAGTCTATGTCATGATGTCGGGGTTGGATTTGGAAAGGCTTGTTTTATCAGGTGGTCCCCTTGGTATTATGCAGGCATGTCTCGATGTTGTCCTTCCTTATACTCGACAACGAGAGCAGTTTGGTCATCCTATTGGAGAGTTTCAGTTCATCCAGGGAAAAGTTGCAGACATGTATACGTCTTTACAGTCCTCAAGGTCTTACGTGTATTCTGTAGCTCGGGACTGTGATAACGGAAAAGTTGACCCGAAGGATTGTGCTGGAGTTATACTCTGTGCAGCTGAAAGAGCAACGCAGGTTGCTTTGCAGGCGATACAATGTTTAGGCGGGAACGGTTATGTGAATGAATATCCAACCGGTCGTTTCCTTAGAGATGCTAAACTCTACGAGATCGGAGCCGGAACTAGTGAGATCAGAAGAATGATTATAGGACGCGAGCTCTTCAAGGAGCAATGACATGCTAGTCCTTTGGATTTTGACTGGAACTGAGCCTCTTGGAGATGATTCTTTGTAAAAGAATTTTTCATTAATCAAATGAGAATTGTGTTAACCAAAATTGATCAACAATACAAGAAACAAAAAACTGCCATGTCATATTTGTATGTGCTCTTTTGCTGTGAAAATCTTGTAAGCAATTCAAACAATATTTTCTAATTTTCTGTTAGAAATTAGTATCTATATTTGATAAATTTTAGAAGACAAACAACATATTTNACTAACNG
TARGET=284,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03434 Ps_contig_mira-and-tgicl-ass_franssen_29992
SEQUENCE=TTTTTCTYACCCCAAACCTTGAGGTCCAACATGGACTTCGAAGAGACACCTTATATTTTGTTTTTCTTCATGAATGAAAGCACACCAAGTTTACATTCCACGGAAAAATATTTCCCGCATTCAATTTCGAAAAGGCATAGAAAGGACATCAGAAAATGAAGTAAAGACATTCATGATGTTTATGGTACCCTCACTGTGCTTTTTTCAGGATTGGAGGGCTTCAAACAAATTCTAGAGTACAAATGACATTTTACATGTTTGAAGCACTCAAGGCCATCATCTTACACTGTTCATTCAATGCCAGAAGTTGAGCTTCTTTCTTGTCAAGCAAGGTACTCAGCTTCACATTCTCTTCCATAAGTTTCTGAACTTCAGCCTCCTTCTGCATTTTCTCACCCTCCAACTGGGTAGTCTTTCCAACTAGCCGTTCAAACAGCTTCTCAATAGTTTGGAATTGCTTCTCGGAAGATAGTAAAGTTTCTCTCACTGTGATTAGACTACTAACATATGATTTGAGAGAAACAAATTCCTGTTTCACTCTACAAAGCTCTTCTTCATTTTCAGCAGCTCGCTCTCTCTGTCTAGTTGTTTCTTCCACCAAATCCGCAACTCTTTGCCTCATGTCATTCAAAATACTATTTGTTCCCAAAGCAAAACTCTCCATTTCTTCCAATTTCATAGACAAATTCTCTAATTGAGCAGACTTTCGCATAATTTCTTCCTGACCTAAACTTACTTTTTCCTTCTCGATTGTTGTTTCCGATTCTGCCAACAAAACCCTTTTGACAAGGGCTTCCATCACATCAGTGACCATCTGAACAGAGTTCAGCATCTCACCTATATAAACTGCATCTTGTTCATCTAACGAACCTTGATGACGGCTAATTCCTATCCAAGATTCTTCTTCGTCTTTCCCATCAAACGAAGAAAGATCAACATTCCTAGACCAACCCGATAACGGAATCCCGTCTTTATCAACAAGCCCTACTCCATCTAATTTCTTGATGCCGCAAGACGTATGTGCTAGATGAGGAATAGCCATGATTCTAGCTTTTGATTTCAAGTACGTAAGCAACGTATTAGTAGTCCTTACTCTTTGCCACAAAACCTCTAATTCCTTTTTCGAGTCTTCCTCCGATCCATGTATACAAGCCTTTATCTCCTTAAGCTTTTCTTGCAATACATCGACTTGAGATTGGTTTCTTTCCATCCCTTCCTTCCATGTTTCATGCGATTGACTCAGCGGAGACTTCAAACCCGACATTGCAACATCTTCACCCTCTGCCATTTCCTGAAAAGGGCGAAGAAGAAGAAGATGAGGGTGTATGGTGGATCGTGGGGGTACAGTAGCAAAACGCTCGTGCCTATCTTTCTCTTAAGTCRAGAAAAAGAAACATCACAAGTGCTTTATTTTTTGTTTTT
TARGET=1303,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03435 Ps_contig_mira-and-tgicl-ass_franssen_29993
SEQUENCE=GGGGAGGTGCAATTAGCAATTTAACNTACTTTACAAAAGCCAATTCACTCTGACTGCTACTTTCTTCTTCTCTTCTTTTCTCTCTTCGAGGGTTTCGTTTTCTCCCTTGAAGAAGAAAAATCCTTTGGTTTTCGTTTTAAGGTGCATCAGTCATGTTTGGGCGTGGACCAACAAGGAAGAGTGATAACACCAAATATTATGACATTCTTGGTGTTTCAAAAAGTGCCGGTGAAGATGAAATCAAGAAAGCCTATAGAAAGGCAGCGATGAAGAACCATCCCGATAAGGGTGGAGATCCTGAGAAGTTCAAGGAGTTGGGTCAAGCATATGAAGTGTTAAGTGATCCTGAAAAGAAAGAACTGTATGATCAATATGGTGAAGATGCCCTGAAAGAAGGAATGGGAGGAGGAGGAGGAAGCTCGTTTCATAATCCTTTTGATCTATTTGAATCATTTTTTGGTGGAGCTGGCTTTGGTGGTGGTGGTCCTTCAAGGGGAAGAAGACAGAAGCAAGGAGAAGATGTGGTGCATTCTTTACAAGTTTCCTTGGAGGATGTGTTTAATGGCACTACAAAGAAACTATCTCTTTCTAGGAATGTGTTGTGCGTAAAATGTAAAGGGAAAGGTTCAAAAAGTGGCACAGCTGGAAGGTGTTTTGGATGCCAAGGCACTGGTATGAAGATTACCAGAAGGCAGATTGGTCTGGGCATGATTCAACAAATGCAACACGCCTGTGTTGACTGCAAAGGAACTGGTGAGGTCATTAGTGAGAGAGATAGATGTCCTCAATGCAAGGCAAACAAGATTACTCAAGAAAAGAAGGTGCTGGAGGTGCATGTGGAAAAGGGAATGCAGCAGGGTCAAAAGATTGTATTTGAAGGACAAGCTGATGAAGCTCCGGACACAATCACCGGAGACATAGTTTTTGTCTTGCAAGTGAAAGTGCATCCAAAGTTCAAGAGGGAGCACGAGGACCTCTATATTGATCACAATCTGAGCTTAACTGAGGCTCTCTGTGGCTTCCAATTTACCGTCACACATCTTGATGGAAGGCAACTCTTGGTCAAATCAAACCCTGGAGAAGTAATCAAGCCAGGTCAATACAAAGCTATTAATGACGAGGGAATGCCACAACACAATAGGCCCTTCATGAGGGGACGCCTTTACATCAAATTTAATGTTGAATTCCCAGAATCAGGTTTTCTTTCACCTAGCCAAAGCCTGGAATTTGAGAAGATATTACCTCAAAAGACAAGCAAGAAGTTGTCGCAAAAGGAACTAGATGATTGTGAGGAGACCACCATGCATGATGTTGATATTGCAGGGGTGCATAGAAAGAAACACCAATATCGCGAGGCATATGATGATGATGACGATGAAGAGGAGCACTCAGGGCCTCGGGTACAGTGTCCGCAACAGTAGTGCAATTTATGATGAAAGTATTACTAAAATGTTCTCTATGATTTTCTGTTGTTTGCATAATGCAGTTATAGCTGCAGCTTAGTTTACCCTAGGTTTCATCCGGAAATGTTACTTTATTTACGATTATATTGTTTGATTTGAATTTTGCTGTAATAGGAGTATCTCTATTTAGCATATGAAAATTAAATGCTTGGTACAAGGTGTGCAATTTTTTAAATTCGATGGAGGATTAAGTGGATCCACAGGATTAGAGCTGTTAAAATGTGACAAAATGGGTCTGACCCATTGTGCAGGCCTACCAGGCATCTATTTTAAGGGATTACCTCTCCCACTCCTAGTGGTGGAGGAGAAGCATCTTATGAAATTAAAAAAATAGCCTTCTTAATTTTAGAAGTATATTTTTGGATGCACATTTCACAA
TARGET=402,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2849
PRIMER_LEFT_PENALTY=0.145769
PRIMER_RIGHT_PENALTY=0.139083
PRIMER_LEFT_SEQUENCE=AGAAGTTCAAGGAGTTGGGTCA
PRIMER_RIGHT_SEQUENCE=AGAATGCACCACATCTTCTCCT
PRIMER_LEFT=300,22
PRIMER_RIGHT=532,22
PRIMER_LEFT_TM=60.146
PRIMER_RIGHT_TM=60.139
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.2849
PRIMER_LEFT_1_PENALTY=0.145769
PRIMER_RIGHT_1_PENALTY=0.139083
PRIMER_LEFT_1_SEQUENCE=TGAGAAGTTCAAGGAGTTGGGT
PRIMER_RIGHT_1_SEQUENCE=AGAATGCACCACATCTTCTCCT
PRIMER_LEFT_1=298,22
PRIMER_RIGHT_1=532,22
PRIMER_LEFT_1_TM=60.146
PRIMER_RIGHT_1_TM=60.139
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.2879
PRIMER_LEFT_2_PENALTY=0.145769
PRIMER_RIGHT_2_PENALTY=0.142170
PRIMER_LEFT_2_SEQUENCE=TGAGAAGTTCAAGGAGTTGGGT
PRIMER_RIGHT_2_SEQUENCE=CTTCTGTCTTCTTCCCCTTGAA
PRIMER_LEFT_2=298,22
PRIMER_RIGHT_2=508,22
PRIMER_LEFT_2_TM=60.146
PRIMER_RIGHT_2_TM=59.858
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.3404
PRIMER_LEFT_3_PENALTY=0.201288
PRIMER_RIGHT_3_PENALTY=0.139083
PRIMER_LEFT_3_SEQUENCE=GATGAAGAACCATCCCGATAAG
PRIMER_RIGHT_3_SEQUENCE=AGAATGCACCACATCTTCTCCT
PRIMER_LEFT_3=265,22
PRIMER_RIGHT_3=532,22
PRIMER_LEFT_3_TM=59.799
PRIMER_RIGHT_3_TM=60.139
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.3435
PRIMER_LEFT_4_PENALTY=0.201288
PRIMER_RIGHT_4_PENALTY=0.142170
PRIMER_LEFT_4_SEQUENCE=GATGAAGAACCATCCCGATAAG
PRIMER_RIGHT_4_SEQUENCE=CTTCTGTCTTCTTCCCCTTGAA
PRIMER_LEFT_4=265,22
PRIMER_RIGHT_4=508,22
PRIMER_LEFT_4_TM=59.799
PRIMER_RIGHT_4_TM=59.858
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=244
=
PRIMER_SEQUENCE_ID=SSR_03436 Ps_contig_mira-and-tgicl-ass_franssen_29993
SEQUENCE=GGGGAGGTGCAATTAGCAATTTAACNTACTTTACAAAAGCCAATTCACTCTGACTGCTACTTTCTTCTTCTCTTCTTTTCTCTCTTCGAGGGTTTCGTTTTCTCCCTTGAAGAAGAAAAATCCTTTGGTTTTCGTTTTAAGGTGCATCAGTCATGTTTGGGCGTGGACCAACAAGGAAGAGTGATAACACCAAATATTATGACATTCTTGGTGTTTCAAAAAGTGCCGGTGAAGATGAAATCAAGAAAGCCTATAGAAAGGCAGCGATGAAGAACCATCCCGATAAGGGTGGAGATCCTGAGAAGTTCAAGGAGTTGGGTCAAGCATATGAAGTGTTAAGTGATCCTGAAAAGAAAGAACTGTATGATCAATATGGTGAAGATGCCCTGAAAGAAGGAATGGGAGGAGGAGGAGGAAGCTCGTTTCATAATCCTTTTGATCTATTTGAATCATTTTTTGGTGGAGCTGGCTTTGGTGGTGGTGGTCCTTCAAGGGGAAGAAGACAGAAGCAAGGAGAAGATGTGGTGCATTCTTTACAAGTTTCCTTGGAGGATGTGTTTAATGGCACTACAAAGAAACTATCTCTTTCTAGGAATGTGTTGTGCGTAAAATGTAAAGGGAAAGGTTCAAAAAGTGGCACAGCTGGAAGGTGTTTTGGATGCCAAGGCACTGGTATGAAGATTACCAGAAGGCAGATTGGTCTGGGCATGATTCAACAAATGCAACACGCCTGTGTTGACTGCAAAGGAACTGGTGAGGTCATTAGTGAGAGAGATAGATGTCCTCAATGCAAGGCAAACAAGATTACTCAAGAAAAGAAGGTGCTGGAGGTGCATGTGGAAAAGGGAATGCAGCAGGGTCAAAAGATTGTATTTGAAGGACAAGCTGATGAAGCTCCGGACACAATCACCGGAGACATAGTTTTTGTCTTGCAAGTGAAAGTGCATCCAAAGTTCAAGAGGGAGCACGAGGACCTCTATATTGATCACAATCTGAGCTTAACTGAGGCTCTCTGTGGCTTCCAATTTACCGTCACACATCTTGATGGAAGGCAACTCTTGGTCAAATCAAACCCTGGAGAAGTAATCAAGCCAGGTCAATACAAAGCTATTAATGACGAGGGAATGCCACAACACAATAGGCCCTTCATGAGGGGACGCCTTTACATCAAATTTAATGTTGAATTCCCAGAATCAGGTTTTCTTTCACCTAGCCAAAGCCTGGAATTTGAGAAGATATTACCTCAAAAGACAAGCAAGAAGTTGTCGCAAAAGGAACTAGATGATTGTGAGGAGACCACCATGCATGATGTTGATATTGCAGGGGTGCATAGAAAGAAACACCAATATCGCGAGGCATATGATGATGATGACGATGAAGAGGAGCACTCAGGGCCTCGGGTACAGTGTCCGCAACAGTAGTGCAATTTATGATGAAAGTATTACTAAAATGTTCTCTATGATTTTCTGTTGTTTGCATAATGCAGTTATAGCTGCAGCTTAGTTTACCCTAGGTTTCATCCGGAAATGTTACTTTATTTACGATTATATTGTTTGATTTGAATTTTGCTGTAATAGGAGTATCTCTATTTAGCATATGAAAATTAAATGCTTGGTACAAGGTGTGCAATTTTTTAAATTCGATGGAGGATTAAGTGGATCCACAGGATTAGAGCTGTTAAAATGTGACAAAATGGGTCTGACCCATTGTGCAGGCCTACCAGGCATCTATTTTAAGGGATTACCTCTCCCACTCCTAGTGGTGGAGGAGAAGCATCTTATGAAATTAAAAAAATAGCCTTCTTAATTTTAGAAGTATATTTTTGGATGCACATTTCACAA
TARGET=473,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2739
PRIMER_LEFT_PENALTY=0.143555
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=GAGGAGGAAGCTCGTTTCATAA
PRIMER_RIGHT_SEQUENCE=AGACCAATCTGCCTTCTGGTAA
PRIMER_LEFT=408,22
PRIMER_RIGHT=702,22
PRIMER_LEFT_TM=59.856
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=0.2849
PRIMER_LEFT_1_PENALTY=0.145769
PRIMER_RIGHT_1_PENALTY=0.139083
PRIMER_LEFT_1_SEQUENCE=AGAAGTTCAAGGAGTTGGGTCA
PRIMER_RIGHT_1_SEQUENCE=AGAATGCACCACATCTTCTCCT
PRIMER_LEFT_1=300,22
PRIMER_RIGHT_1=532,22
PRIMER_LEFT_1_TM=60.146
PRIMER_RIGHT_1_TM=60.139
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.2849
PRIMER_LEFT_2_PENALTY=0.145769
PRIMER_RIGHT_2_PENALTY=0.139083
PRIMER_LEFT_2_SEQUENCE=TGAGAAGTTCAAGGAGTTGGGT
PRIMER_RIGHT_2_SEQUENCE=AGAATGCACCACATCTTCTCCT
PRIMER_LEFT_2=298,22
PRIMER_RIGHT_2=532,22
PRIMER_LEFT_2_TM=60.146
PRIMER_RIGHT_2_TM=60.139
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=235
PRIMER_PAIR_PENALTY_3=0.2857
PRIMER_LEFT_3_PENALTY=0.143555
PRIMER_RIGHT_3_PENALTY=0.142170
PRIMER_LEFT_3_SEQUENCE=GAGGAGGAAGCTCGTTTCATAA
PRIMER_RIGHT_3_SEQUENCE=CTTCTGTCTTCTTCCCCTTGAA
PRIMER_LEFT_3=408,22
PRIMER_RIGHT_3=508,22
PRIMER_LEFT_3_TM=59.856
PRIMER_RIGHT_3_TM=59.858
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=0.2879
PRIMER_LEFT_4_PENALTY=0.145769
PRIMER_RIGHT_4_PENALTY=0.142170
PRIMER_LEFT_4_SEQUENCE=TGAGAAGTTCAAGGAGTTGGGT
PRIMER_RIGHT_4_SEQUENCE=CTTCTGTCTTCTTCCCCTTGAA
PRIMER_LEFT_4=298,22
PRIMER_RIGHT_4=508,22
PRIMER_LEFT_4_TM=60.146
PRIMER_RIGHT_4_TM=59.858
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_03437 Ps_contig_mira-and-tgicl-ass_franssen_29993
SEQUENCE=GGGGAGGTGCAATTAGCAATTTAACNTACTTTACAAAAGCCAATTCACTCTGACTGCTACTTTCTTCTTCTCTTCTTTTCTCTCTTCGAGGGTTTCGTTTTCTCCCTTGAAGAAGAAAAATCCTTTGGTTTTCGTTTTAAGGTGCATCAGTCATGTTTGGGCGTGGACCAACAAGGAAGAGTGATAACACCAAATATTATGACATTCTTGGTGTTTCAAAAAGTGCCGGTGAAGATGAAATCAAGAAAGCCTATAGAAAGGCAGCGATGAAGAACCATCCCGATAAGGGTGGAGATCCTGAGAAGTTCAAGGAGTTGGGTCAAGCATATGAAGTGTTAAGTGATCCTGAAAAGAAAGAACTGTATGATCAATATGGTGAAGATGCCCTGAAAGAAGGAATGGGAGGAGGAGGAGGAAGCTCGTTTCATAATCCTTTTGATCTATTTGAATCATTTTTTGGTGGAGCTGGCTTTGGTGGTGGTGGTCCTTCAAGGGGAAGAAGACAGAAGCAAGGAGAAGATGTGGTGCATTCTTTACAAGTTTCCTTGGAGGATGTGTTTAATGGCACTACAAAGAAACTATCTCTTTCTAGGAATGTGTTGTGCGTAAAATGTAAAGGGAAAGGTTCAAAAAGTGGCACAGCTGGAAGGTGTTTTGGATGCCAAGGCACTGGTATGAAGATTACCAGAAGGCAGATTGGTCTGGGCATGATTCAACAAATGCAACACGCCTGTGTTGACTGCAAAGGAACTGGTGAGGTCATTAGTGAGAGAGATAGATGTCCTCAATGCAAGGCAAACAAGATTACTCAAGAAAAGAAGGTGCTGGAGGTGCATGTGGAAAAGGGAATGCAGCAGGGTCAAAAGATTGTATTTGAAGGACAAGCTGATGAAGCTCCGGACACAATCACCGGAGACATAGTTTTTGTCTTGCAAGTGAAAGTGCATCCAAAGTTCAAGAGGGAGCACGAGGACCTCTATATTGATCACAATCTGAGCTTAACTGAGGCTCTCTGTGGCTTCCAATTTACCGTCACACATCTTGATGGAAGGCAACTCTTGGTCAAATCAAACCCTGGAGAAGTAATCAAGCCAGGTCAATACAAAGCTATTAATGACGAGGGAATGCCACAACACAATAGGCCCTTCATGAGGGGACGCCTTTACATCAAATTTAATGTTGAATTCCCAGAATCAGGTTTTCTTTCACCTAGCCAAAGCCTGGAATTTGAGAAGATATTACCTCAAAAGACAAGCAAGAAGTTGTCGCAAAAGGAACTAGATGATTGTGAGGAGACCACCATGCATGATGTTGATATTGCAGGGGTGCATAGAAAGAAACACCAATATCGCGAGGCATATGATGATGATGACGATGAAGAGGAGCACTCAGGGCCTCGGGTACAGTGTCCGCAACAGTAGTGCAATTTATGATGAAAGTATTACTAAAATGTTCTCTATGATTTTCTGTTGTTTGCATAATGCAGTTATAGCTGCAGCTTAGTTTACCCTAGGTTTCATCCGGAAATGTTACTTTATTTACGATTATATTGTTTGATTTGAATTTTGCTGTAATAGGAGTATCTCTATTTAGCATATGAAAATTAAATGCTTGGTACAAGGTGTGCAATTTTTTAAATTCGATGGAGGATTAAGTGGATCCACAGGATTAGAGCTGTTAAAATGTGACAAAATGGGTCTGACCCATTGTGCAGGCCTACCAGGCATCTATTTTAAGGGATTACCTCTCCCACTCCTAGTGGTGGAGGAGAAGCATCTTATGAAATTAAAAAAATAGCCTTCTTAATTTTAGAAGTATATTTTTGGATGCACATTTCACAA
TARGET=1360,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7702
PRIMER_LEFT_PENALTY=0.101479
PRIMER_RIGHT_PENALTY=0.668761
PRIMER_LEFT_SEQUENCE=TTTTCTTTCACCTAGCCAAAGC
PRIMER_RIGHT_SEQUENCE=TAAATTGCACTACTGTTGCGGA
PRIMER_LEFT=1198,22
PRIMER_RIGHT=1429,22
PRIMER_LEFT_TM=59.899
PRIMER_RIGHT_TM=60.669
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.7860
PRIMER_LEFT_1_PENALTY=0.117281
PRIMER_RIGHT_1_PENALTY=0.668761
PRIMER_LEFT_1_SEQUENCE=TGTCGCAAAAGGAACTAGATGA
PRIMER_RIGHT_1_SEQUENCE=TAAATTGCACTACTGTTGCGGA
PRIMER_LEFT_1=1263,22
PRIMER_RIGHT_1=1429,22
PRIMER_LEFT_1_TM=59.883
PRIMER_RIGHT_1_TM=60.669
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.8643
PRIMER_LEFT_2_PENALTY=0.195574
PRIMER_RIGHT_2_PENALTY=0.668761
PRIMER_LEFT_2_SEQUENCE=AAGAAGTTGTCGCAAAAGGAAC
PRIMER_RIGHT_2_SEQUENCE=TAAATTGCACTACTGTTGCGGA
PRIMER_LEFT_2=1256,22
PRIMER_RIGHT_2=1429,22
PRIMER_LEFT_2_TM=59.804
PRIMER_RIGHT_2_TM=60.669
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.8717
PRIMER_LEFT_3_PENALTY=0.117281
PRIMER_RIGHT_3_PENALTY=0.754440
PRIMER_LEFT_3_SEQUENCE=TGTCGCAAAAGGAACTAGATGA
PRIMER_RIGHT_3_SEQUENCE=GTAACATTTCCGGATGAAACCT
PRIMER_LEFT_3=1263,22
PRIMER_RIGHT_3=1532,22
PRIMER_LEFT_3_TM=59.883
PRIMER_RIGHT_3_TM=59.246
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.8717
PRIMER_LEFT_4_PENALTY=0.117281
PRIMER_RIGHT_4_PENALTY=0.754440
PRIMER_LEFT_4_SEQUENCE=TGTCGCAAAAGGAACTAGATGA
PRIMER_RIGHT_4_SEQUENCE=AGTAACATTTCCGGATGAAACC
PRIMER_LEFT_4=1263,22
PRIMER_RIGHT_4=1533,22
PRIMER_LEFT_4_TM=59.883
PRIMER_RIGHT_4_TM=59.246
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_03438 Ps_contig_mira-and-tgicl-ass_franssen_29994
SEQUENCE=TTAGTTTTTCTTTTGTTAGGTGCCAACTTAAACAACACTCCTCCAAGAACGTTAGTAAAGAATGCACCACATCTTCTCCTTGCTTCTGTCTTCCTTCCCTTNAGGACCACCACCACCAAAGCCCAGCTCCACCAAAAAAATAGATTCAAATAGATCAAAAGGATTATGAAACGAGCTTCCTCCTCCTCCTCCCATTCCTTCTTTCAGGGCATCTTCACCATATTGATCATACAGTTCTTTCTTTTACAGGATCACTTAACACTTCATATGCTTGACCCAACTCCTTGAACTTCTCAGGATCTCCACCCTTATCGGGATAGGTTCTTCATCGCTGCCTTCTATAGGTTTCTTGATTTCATCTTCACCGGCTACTTTTGAAACACCAAGAATGTCATAATTATTTGGTGTTATCACTCTTCTTGTTGTCCACGACCCAAACATGACTGATGACCAACC
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2556
PRIMER_LEFT_PENALTY=0.139083
PRIMER_RIGHT_PENALTY=0.116503
PRIMER_LEFT_SEQUENCE=AGAATGCACCACATCTTCTCCT
PRIMER_RIGHT_SEQUENCE=ATAGAAGGCAGCGATGAAGAAC
PRIMER_LEFT=58,22
PRIMER_RIGHT=341,22
PRIMER_LEFT_TM=60.139
PRIMER_RIGHT_TM=59.883
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.2849
PRIMER_LEFT_1_PENALTY=0.139083
PRIMER_RIGHT_1_PENALTY=0.145769
PRIMER_LEFT_1_SEQUENCE=AGAATGCACCACATCTTCTCCT
PRIMER_RIGHT_1_SEQUENCE=AGAAGTTCAAGGAGTTGGGTCA
PRIMER_LEFT_1=58,22
PRIMER_RIGHT_1=293,22
PRIMER_LEFT_1_TM=60.139
PRIMER_RIGHT_1_TM=60.146
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.2849
PRIMER_LEFT_2_PENALTY=0.139083
PRIMER_RIGHT_2_PENALTY=0.145769
PRIMER_LEFT_2_SEQUENCE=AGAATGCACCACATCTTCTCCT
PRIMER_RIGHT_2_SEQUENCE=TGAGAAGTTCAAGGAGTTGGGT
PRIMER_LEFT_2=58,22
PRIMER_RIGHT_2=295,22
PRIMER_LEFT_2_TM=60.139
PRIMER_RIGHT_2_TM=60.146
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.3830
PRIMER_LEFT_3_PENALTY=0.139083
PRIMER_RIGHT_3_PENALTY=0.243965
PRIMER_LEFT_3_SEQUENCE=AGAATGCACCACATCTTCTCCT
PRIMER_RIGHT_3_SEQUENCE=ACCTATAGAAGGCAGCGATGAA
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=345,22
PRIMER_LEFT_3_TM=60.139
PRIMER_RIGHT_3_TM=60.244
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.3830
PRIMER_LEFT_4_PENALTY=0.139083
PRIMER_RIGHT_4_PENALTY=0.243965
PRIMER_LEFT_4_SEQUENCE=AGAATGCACCACATCTTCTCCT
PRIMER_RIGHT_4_SEQUENCE=AACCTATAGAAGGCAGCGATGA
PRIMER_LEFT_4=58,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=60.139
PRIMER_RIGHT_4_TM=60.244
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_03439 Ps_contig_mira-and-tgicl-ass_franssen_29994
SEQUENCE=TTAGTTTTTCTTTTGTTAGGTGCCAACTTAAACAACACTCCTCCAAGAACGTTAGTAAAGAATGCACCACATCTTCTCCTTGCTTCTGTCTTCCTTCCCTTNAGGACCACCACCACCAAAGCCCAGCTCCACCAAAAAAATAGATTCAAATAGATCAAAAGGATTATGAAACGAGCTTCCTCCTCCTCCTCCCATTCCTTCTTTCAGGGCATCTTCACCATATTGATCATACAGTTCTTTCTTTTACAGGATCACTTAACACTTCATATGCTTGACCCAACTCCTTGAACTTCTCAGGATCTCCACCCTTATCGGGATAGGTTCTTCATCGCTGCCTTCTATAGGTTTCTTGATTTCATCTTCACCGGCTACTTTTGAAACACCAAGAATGTCATAATTATTTGGTGTTATCACTCTTCTTGTTGTCCACGACCCAAACATGACTGATGACCAACC
TARGET=178,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2556
PRIMER_LEFT_PENALTY=0.139083
PRIMER_RIGHT_PENALTY=0.116503
PRIMER_LEFT_SEQUENCE=AGAATGCACCACATCTTCTCCT
PRIMER_RIGHT_SEQUENCE=ATAGAAGGCAGCGATGAAGAAC
PRIMER_LEFT=58,22
PRIMER_RIGHT=341,22
PRIMER_LEFT_TM=60.139
PRIMER_RIGHT_TM=59.883
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.2571
PRIMER_LEFT_1_PENALTY=0.140609
PRIMER_RIGHT_1_PENALTY=0.116503
PRIMER_LEFT_1_SEQUENCE=CTGTCTTCCTTCCCTTNAGGAC
PRIMER_RIGHT_1_SEQUENCE=ATAGAAGGCAGCGATGAAGAAC
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=341,22
PRIMER_LEFT_1_TM=59.859
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.50
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.50
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.2697
PRIMER_LEFT_2_PENALTY=0.140609
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=CTGTCTTCCTTCCCTTNAGGAC
PRIMER_RIGHT_2_SEQUENCE=AAAGTAGCCGGTGAAGATGAAA
PRIMER_LEFT_2=85,22
PRIMER_RIGHT_2=374,22
PRIMER_LEFT_2_TM=59.859
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.50
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.50
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.2697
PRIMER_LEFT_3_PENALTY=0.140609
PRIMER_RIGHT_3_PENALTY=0.129103
PRIMER_LEFT_3_SEQUENCE=CTGTCTTCCTTCCCTTNAGGAC
PRIMER_RIGHT_3_SEQUENCE=AAAAGTAGCCGGTGAAGATGAA
PRIMER_LEFT_3=85,22
PRIMER_RIGHT_3=375,22
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=60.129
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.50
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.50
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.2697
PRIMER_LEFT_4_PENALTY=0.140609
PRIMER_RIGHT_4_PENALTY=0.129103
PRIMER_LEFT_4_SEQUENCE=CTGTCTTCCTTCCCTTNAGGAC
PRIMER_RIGHT_4_SEQUENCE=TTCAAAAGTAGCCGGTGAAGAT
PRIMER_LEFT_4=85,22
PRIMER_RIGHT_4=378,22
PRIMER_LEFT_4_TM=59.859
PRIMER_RIGHT_4_TM=60.129
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.50
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.50
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_03440 Ps_contig_mira-and-tgicl-ass_franssen_30001
SEQUENCE=AAAGACAGTGGTATCAACGCAGAGTTCTCATTTCTCTTTCTCTTACTCACTCCACTTCTAGAGAGAGAAAAACCAAACAATAATTAATGTGTTTTCCTCTTCTCTATTGAGATCATATTTGTCGGTGGAATTTCCATTTTCATAGTTGACACAACAACACAAGCCTTTGAGTATGTACGGAAGAGATCCATGGGGAGGTCCATTAGAGATCAACGCAACAGACTCAGCAACAGATGAAGATCGTAGTAGGAATCTTCAAGAGCTAGATAGAGCAGCGTTGTCTCGTCCTTTAGATGAAACTCAACAGAGTTGGTTGTTGGGTCCTACTGAACAGAAGAAGAAGAAGAAGTATGTAGATCTGGGTTGTATCATTGTTAGCCGCAAGATCTTTGTTTGGACTGTTGGGACTATTCTCTGTGCTGCTGCTCTTGCTGGTTTGGTTGTTCTTATTGTTAAAACTGTTCCTCGCCATCATCATAAACATCCTCCTCCGGATAATTATACTCTTGCTCTTCATAAAGCTCTCATGTTCTTCAATGCACAAAAATCTGGGAAACTTCCGAAGCATAACAATGTTTCTTGGAGGGGAACTTCGTGTATGCAAGATGGAAAGGGTGACGGTGTTTCTGCTGCAATCAAGGATCTGGTTGGTGGATACTATGATGCTGGAGATGCCATCAAGTTCAATTTTCCGGCTGCTTTTTCCATCACTATGTTGAGTTGGAGTGTTATTGAGTATAGTGGTAAATACGAAGCGACCGGGGAGCTTGATCATGTCAAGGAAATCATTAAATGGGGTACCGATTATTTTCTCAAGACATTCAATCATACCGCAGATACCATTACCACCATTGCCGCTCAGGTTGGGAGTGGAGATACCTCAGATGGAAGTACGACTCCCAATGATCACTACTGCTGGATGCGTCCAGAAGACATTGATTACAACAGGCCAGTCACCGAATGTCACAGTTGTTCGGAGCTTGCTGCAGAAATGTCGGCTGCCTTGGCGGCTGCGTCCATTGTTTTCAAAGACAACAAAGCCTATTCCAAGAAACTTGTTCATGGTGCCGGTACACTTTTTAAGTTTTCCAGGGATCAGAGAGGCAGATACAGTGCAGGTAGTGCTGAGTCTGCGACATTCTACAATTCTACCGGCTACTGGGATGAGTTTGTTTGGGGAGGCGCGTGGATGTACTTTGCAACCGGAAATAACTCGTACCTTAGGATTGCTACTAGTCCCGGTATTGCTAAACATGCTGGTGCTTTCTGGGGTGGCCCTGATTATGGAGTACTGAGCTGGGATAACAAGCTTGCTGGTGCACAGGTTCTTCTTAGTCGATTGAGGTTGTTCTTGAGTCCTGGGTATCCATATGAAGAAATTCTAAGGACCTTCCACAATCAGACGAGTATAATCATGTGCTCCTACCTACCTGTTTTCACAAGCTTTAACAGAACCAAAGGAGGCTTGATTCAGTTAAATCATGGTAGGCCTCAGCCTCTCCAATATGTAGTCAATGCAGCCTTTTTGGCTACCCTATACAGTGATTACCTTGATGCCGCCGACACCCCTGGGTGGTATTGTGGACCTAATTTCTATTCAACCGATAAACTCCGCGAGTTTGCCAGGACACAGATTGATTACATCCTTGGGAAGAATCCACGGAAAATGAGCTATATTGTAGGTTTTGGAAATCATTATCCAAAACATGTCCACCATAGAGGTGCATCTATACCAAAGAACAAGGTTAAATATAACTGCAAAGGTGGATGGAAATGGAGGGACACATCAAAGCCAAATCCAAATATACTTGTTGGTGCCATGGTTGCTGGTCCTGACAGGCACGACGGTTTCCACGATGTTCGATCAAACTACAACTACACAGAGCCAACACTTGCTGGAAATGCTGGTTTAGTAGCAGCATTAGTCGCTCTATCAGGTGACAAAAGCATACCGATTGACAAAAACACCCTTTTCTCTGCTGTTCCACCAATGTTTCCAACACCACCACCACCTCCAGCACCATGGAAACCATAAGGTGTCATTTCGTTGGTTATCTTTTTCCACAGCAAGTACCCATAAATGACTTGAGTGCATTGGTAAAACTTCAGATGTTTTATATCTCAACTAAAAAAGTTCAAGGAAAATTTTGAAGACAAGGCAACATGCTTGGCCTAGTGAATCTAGTTGGTTGTGTGGCTAATGGTGACTCGAGAGTAGAATTAAGACTTGATAAAGTGGTACTTGAGAGTGTCTCTTTGTATTTTTCTTTTTGTGATATACATAGTATATTTGTATCAAAACCAAATCTTGTATTCTTTAAACATGAAAACTGAACAGCTTGTAATTGTTTGACAATTTCATCTAATGTGGTTACTGTTTTACTCTTGACCTCTATTTCATATAAAACCTTTCTCTT
TARGET=333,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0652
PRIMER_LEFT_PENALTY=0.038361
PRIMER_RIGHT_PENALTY=0.026872
PRIMER_LEFT_SEQUENCE=GGAGGTCCATTAGAGATCAACG
PRIMER_RIGHT_SEQUENCE=TTGCGGCTAACAATGATACAAC
PRIMER_LEFT=193,22
PRIMER_RIGHT=383,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=60.027
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.0850
PRIMER_LEFT_1_PENALTY=0.058146
PRIMER_RIGHT_1_PENALTY=0.026872
PRIMER_LEFT_1_SEQUENCE=AGAGATCAACGCAACAGACTCA
PRIMER_RIGHT_1_SEQUENCE=TTGCGGCTAACAATGATACAAC
PRIMER_LEFT_1=204,22
PRIMER_RIGHT_1=383,22
PRIMER_LEFT_1_TM=60.058
PRIMER_RIGHT_1_TM=60.027
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.0850
PRIMER_LEFT_2_PENALTY=0.058146
PRIMER_RIGHT_2_PENALTY=0.026872
PRIMER_LEFT_2_SEQUENCE=GAGATCAACGCAACAGACTCAG
PRIMER_RIGHT_2_SEQUENCE=TTGCGGCTAACAATGATACAAC
PRIMER_LEFT_2=205,22
PRIMER_RIGHT_2=383,22
PRIMER_LEFT_2_TM=60.058
PRIMER_RIGHT_2_TM=60.027
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.1010
PRIMER_LEFT_3_PENALTY=0.058146
PRIMER_RIGHT_3_PENALTY=0.042816
PRIMER_LEFT_3_SEQUENCE=AGAGATCAACGCAACAGACTCA
PRIMER_RIGHT_3_SEQUENCE=AATTATCCGGAGGAGGATGTTT
PRIMER_LEFT_3=204,22
PRIMER_RIGHT_3=499,22
PRIMER_LEFT_3_TM=60.058
PRIMER_RIGHT_3_TM=60.043
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.1010
PRIMER_LEFT_4_PENALTY=0.058146
PRIMER_RIGHT_4_PENALTY=0.042816
PRIMER_LEFT_4_SEQUENCE=GAGATCAACGCAACAGACTCAG
PRIMER_RIGHT_4_SEQUENCE=AATTATCCGGAGGAGGATGTTT
PRIMER_LEFT_4=205,22
PRIMER_RIGHT_4=499,22
PRIMER_LEFT_4_TM=60.058
PRIMER_RIGHT_4_TM=60.043
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_03441 Ps_contig_mira-and-tgicl-ass_franssen_30001
SEQUENCE=AAAGACAGTGGTATCAACGCAGAGTTCTCATTTCTCTTTCTCTTACTCACTCCACTTCTAGAGAGAGAAAAACCAAACAATAATTAATGTGTTTTCCTCTTCTCTATTGAGATCATATTTGTCGGTGGAATTTCCATTTTCATAGTTGACACAACAACACAAGCCTTTGAGTATGTACGGAAGAGATCCATGGGGAGGTCCATTAGAGATCAACGCAACAGACTCAGCAACAGATGAAGATCGTAGTAGGAATCTTCAAGAGCTAGATAGAGCAGCGTTGTCTCGTCCTTTAGATGAAACTCAACAGAGTTGGTTGTTGGGTCCTACTGAACAGAAGAAGAAGAAGAAGTATGTAGATCTGGGTTGTATCATTGTTAGCCGCAAGATCTTTGTTTGGACTGTTGGGACTATTCTCTGTGCTGCTGCTCTTGCTGGTTTGGTTGTTCTTATTGTTAAAACTGTTCCTCGCCATCATCATAAACATCCTCCTCCGGATAATTATACTCTTGCTCTTCATAAAGCTCTCATGTTCTTCAATGCACAAAAATCTGGGAAACTTCCGAAGCATAACAATGTTTCTTGGAGGGGAACTTCGTGTATGCAAGATGGAAAGGGTGACGGTGTTTCTGCTGCAATCAAGGATCTGGTTGGTGGATACTATGATGCTGGAGATGCCATCAAGTTCAATTTTCCGGCTGCTTTTTCCATCACTATGTTGAGTTGGAGTGTTATTGAGTATAGTGGTAAATACGAAGCGACCGGGGAGCTTGATCATGTCAAGGAAATCATTAAATGGGGTACCGATTATTTTCTCAAGACATTCAATCATACCGCAGATACCATTACCACCATTGCCGCTCAGGTTGGGAGTGGAGATACCTCAGATGGAAGTACGACTCCCAATGATCACTACTGCTGGATGCGTCCAGAAGACATTGATTACAACAGGCCAGTCACCGAATGTCACAGTTGTTCGGAGCTTGCTGCAGAAATGTCGGCTGCCTTGGCGGCTGCGTCCATTGTTTTCAAAGACAACAAAGCCTATTCCAAGAAACTTGTTCATGGTGCCGGTACACTTTTTAAGTTTTCCAGGGATCAGAGAGGCAGATACAGTGCAGGTAGTGCTGAGTCTGCGACATTCTACAATTCTACCGGCTACTGGGATGAGTTTGTTTGGGGAGGCGCGTGGATGTACTTTGCAACCGGAAATAACTCGTACCTTAGGATTGCTACTAGTCCCGGTATTGCTAAACATGCTGGTGCTTTCTGGGGTGGCCCTGATTATGGAGTACTGAGCTGGGATAACAAGCTTGCTGGTGCACAGGTTCTTCTTAGTCGATTGAGGTTGTTCTTGAGTCCTGGGTATCCATATGAAGAAATTCTAAGGACCTTCCACAATCAGACGAGTATAATCATGTGCTCCTACCTACCTGTTTTCACAAGCTTTAACAGAACCAAAGGAGGCTTGATTCAGTTAAATCATGGTAGGCCTCAGCCTCTCCAATATGTAGTCAATGCAGCCTTTTTGGCTACCCTATACAGTGATTACCTTGATGCCGCCGACACCCCTGGGTGGTATTGTGGACCTAATTTCTATTCAACCGATAAACTCCGCGAGTTTGCCAGGACACAGATTGATTACATCCTTGGGAAGAATCCACGGAAAATGAGCTATATTGTAGGTTTTGGAAATCATTATCCAAAACATGTCCACCATAGAGGTGCATCTATACCAAAGAACAAGGTTAAATATAACTGCAAAGGTGGATGGAAATGGAGGGACACATCAAAGCCAAATCCAAATATACTTGTTGGTGCCATGGTTGCTGGTCCTGACAGGCACGACGGTTTCCACGATGTTCGATCAAACTACAACTACACAGAGCCAACACTTGCTGGAAATGCTGGTTTAGTAGCAGCATTAGTCGCTCTATCAGGTGACAAAAGCATACCGATTGACAAAAACACCCTTTTCTCTGCTGTTCCACCAATGTTTCCAACACCACCACCACCTCCAGCACCATGGAAACCATAAGGTGTCATTTCGTTGGTTATCTTTTTCCACAGCAAGTACCCATAAATGACTTGAGTGCATTGGTAAAACTTCAGATGTTTTATATCTCAACTAAAAAAGTTCAAGGAAAATTTTGAAGACAAGGCAACATGCTTGGCCTAGTGAATCTAGTTGGTTGTGTGGCTAATGGTGACTCGAGAGTAGAATTAAGACTTGATAAAGTGGTACTTGAGAGTGTCTCTTTGTATTTTTCTTTTTGTGATATACATAGTATATTTGTATCAAAACCAAATCTTGTATTCTTTAAACATGAAAACTGAACAGCTTGTAATTGTTTGACAATTTCATCTAATGTGGTTACTGTTTTACTCTTGACCTCTATTTCATATAAAACCTTTCTCTT
TARGET=2001,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0405
PRIMER_LEFT_PENALTY=0.003166
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=TGACAAAAGCATACCGATTGAC
PRIMER_RIGHT_SEQUENCE=GCCACACAACCAACTAGATTCA
PRIMER_LEFT=1938,22
PRIMER_RIGHT=2197,22
PRIMER_LEFT_TM=60.003
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.0405
PRIMER_LEFT_1_PENALTY=0.003166
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=GTGACAAAAGCATACCGATTGA
PRIMER_RIGHT_1_SEQUENCE=GCCACACAACCAACTAGATTCA
PRIMER_LEFT_1=1937,22
PRIMER_RIGHT_1=2197,22
PRIMER_LEFT_1_TM=60.003
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.0405
PRIMER_LEFT_2_PENALTY=0.003166
PRIMER_RIGHT_2_PENALTY=0.037339
PRIMER_LEFT_2_SEQUENCE=GACAAAAGCATACCGATTGACA
PRIMER_RIGHT_2_SEQUENCE=GCCACACAACCAACTAGATTCA
PRIMER_LEFT_2=1939,22
PRIMER_RIGHT_2=2197,22
PRIMER_LEFT_2_TM=60.003
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.0507
PRIMER_LEFT_3_PENALTY=0.013401
PRIMER_RIGHT_3_PENALTY=0.037339
PRIMER_LEFT_3_SEQUENCE=ATACCGATTGACAAAAACACCC
PRIMER_RIGHT_3_SEQUENCE=GCCACACAACCAACTAGATTCA
PRIMER_LEFT_3=1948,22
PRIMER_RIGHT_3=2197,22
PRIMER_LEFT_3_TM=59.987
PRIMER_RIGHT_3_TM=60.037
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.0835
PRIMER_LEFT_4_PENALTY=0.003166
PRIMER_RIGHT_4_PENALTY=0.080323
PRIMER_LEFT_4_SEQUENCE=TGACAAAAGCATACCGATTGAC
PRIMER_RIGHT_4_SEQUENCE=CCATTAGCCACACAACCAACTA
PRIMER_LEFT_4=1938,22
PRIMER_RIGHT_4=2203,22
PRIMER_LEFT_4_TM=60.003
PRIMER_RIGHT_4_TM=59.920
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_03442 Ps_contig_mira-and-tgicl-ass_franssen_30003
SEQUENCE=ACACACAACCTAAAGACTTCTATCAAATAACTACACACCAACNCTATATACATTGCTTTGTCTCCCAAGTCTTTATTATTACATATTATTACATCCANTAATGACATAGTACTACATAAACAAGAAACAAAAAACCCCAACGAAAATAATTTCTAAATACTGGACATCATTTTATTTCTTCCTTGTACTTCAGTTCCATAGCTAGCACAAGCACATCAACCTTAATCCTTCATTGGTGAAACCAAACTTTCAACATAAACCTCTGCATCAAACCAACCCTTCCCACCTTCAATCCTAGGATACCTAAATGAATGGTCTCTTGGATTATACACAATCAAATCAAACTCAAACATCAACAACACTTCACCATTCTCTGAAATACAATAAGGCCCTGAATATGAAAAATCCTCAGGATTAGGCAAATAAGGAACTGTCACCAATTTAATCCAAGACTCTCTCACACCATAATCCTTCATCAACCAAACAACAAAATCACTCTTTTTGTAATCATAGTTCATGCAAAGACAACCCTTAAGAACACTCAAACTAGGAGTTGAACACTCTTCCTTTTCATAATCAGGTGGAAGAACTTCCCTATAAGTTTCCTTCTCCAAATCCAAAGAAACAATGATCCACAGAGAACTTGAACCATGTGAGTAATTAGAAGCCCAATTGAGAGTACCACTCACAAATTTCCCAGATGAATTTTGATAAGGTGAAACACCATGAGGGAAATCATGAATCTTCCTCCAAGAATTAGTATTCATACTAAAAATCTTAACTTTAGATTCACTGAAAAACTCATTAGGATCACAAAAAACAGCAACAACCTTGTAATCTTCATTGACATGATCATAACCAAGACCAAAAGAAGTGTAACAACCGGGTCTCCAATTATTCCCTAAAGGTGGTGACTTTTTAGAAACCCTAATAGAAGGGTTCCAAAGAAGAACACAATCACCTTTTATAGCAAAACAGAGGAACCCATTACAGGAACCAACAATACCATCATGTCGAAACTTGTTTTTCACAGGGTAGTTAAGGATTTCAGAAACCGTTGATGGGTTATTGAAAAGAGAGGTAATGGAACAAGATTTGAGATGAAATTCAGCTGTTGTAGCACTCAAGATGATTCTATGATGGGTTGTGAAGTGAGTGTTGTATGTTGAAAAGTGAAGTTGCTTTTTTGTGAAGTAAGGGTTGGAAATTATGTGTAACCATGATTTGCATACACACTTGAAACGTAAAAGAGATTTGATTGGAAGTTTTGTGAGGATTTCGAGAATGAGTTCTTCAGGGAGAAAGGGTAATGGAAGTGGCGGTGTCATAGGTAGTGGCGGTGAGTGATAGAGAAGAGAGTGTGAGTTTGAGTGTTGAGAGGTTCATTCATGTGGTTGTTGATGAATGATACTAGTATATAGATATATATATATAGTAGTGAAAGTGAAGATGAAGATGAAGATGAAGATGATGAAAAATAGATTGATATCNGTNTACATTATGATGTATTATGTATTTAATTAATAAAATAAAATAGAAA
TARGET=1422,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03443 Ps_contig_mira-and-tgicl-ass_franssen_30006
SEQUENCE=AACATTTATTTATTTTGGTTATGGCAAAAAATAGTTAATAACTAATCAAAAGAAAAAACTTTACTACACCCAGTATCGANATTTTATTAGTTGAAGTTTGTATTAAAATTTTAATGGTTCTCAGATTTTCTTTTAATACCTGAATGCCATATCATTCCCATATTGTTCTTCATCAAACATTGTCATACCATTCACATATTGTTCTTCATCAAACAATGTGCTCCTATAAGGATTTCTTTTTTTGCCAGTAAAACGGTCGACTTCATACAGCCCAGCCCTTTACAGAAATCAATTGAACACGTCTCAATCTTGGCATGAGTAATTGAAACTTTTGTCTTTAATAAGAATAAAATTACTAGCATATCAATCGGTTTCTACAGCATTTTTGTATCAGTCCTCCAACTGCACAGGCTCTGTGACAGGAGGTATACAATCTACCTTATACCGTAGCACCTTTCTAAAAAAAGTTGAATGTGTGTTGTCCCATATCAGTTTATAGCTTCCAGCCATTAATGTGCAGAAGTTACCTTGGTCAGACTCGTATCGCCGGTGAGGCAACATCAGAGTCTTTTCCCCAGTAGGACTTACAAACTCCAAACTAAATCCAATATCCATATTTATCTTGCCTTGTACTAGAGAAAAGTCCCATGCAATATACGAGTTTACTGAGTCCACTGAGAGTATAACCTCAAAAGTTTTCCCAGCTGGAACACTTGCCTCTTTGAAGTCATCAATTTTACTTACTTCCACTGCACCGGCAACATCTGCTGGAATGAAGAAAGGCGAGTTTGCAAAGACCCCACTAATAAATGTGATGAACCTCTCAAACTCCATGGTGTAGACATCAACAGCTTGTTCCAAGACAGCAAACTGTTCCCTTTTTAATTTCTTATACTCATATCGTGCCCACGATAATGAAAGGAAGAACCGGACATAAAGAATCATTGAGGTGAGGCTGTATAAAGGTGGTCCATAGCGATGACGTTGCTCCTCCATGGGCCCGTCATCGTCATTGATCTGCCTTGTAAAATCAAGCAATACCTCAGGAACTCCAACTAGGTACTTCACGAGGGTTTTATAGACTCCTGTTGCAGAGCCAAGCTGAGCTAATTGCCTTCTCCTGTCCATTTGTTGTTTCCACAGAAGAGCATATCGATCATGTATGCTACCACCAGAATTCACCAAAGCATCTACCTCTGCTTGTTTATTCCTCTCTGAGCGTTCCCGTTGTTGTCTGAGGAGAGTTCCTCGAAAATCTTGAGGCAGATATGTCATAACTGTGTTAAATACATGTTCAGCATTTTTTATTTGGAAAGATTCCAAAGTCCTCAAAGTATTATGAAGTTTATCTTTGAGCTTGGGAAGCATAATGGAAAATTCAACATTGTCAGGAGCGGGCGGGTGTGGGCAATTAGAAGGTTCAAGTAAAAAAATAGTTTCTTCAATGTATTCTCGATTCTTCCACATATTTTCATCAATCTTATGCGGAGGTTGGGTCTGAGCTTCATTTATTAACGTGGTTTCTCCATGGATAGGAGGATGTTGAGTGAGCAAATCAGTGGCCATAGAACGAAGCTCGGAAGAAAGGCGAGAGACATCTGGAGAAAGAGGAGTAAAGGGGTGTTGATCGTAGTAAGACGCCAGAAAGGCCCTAGTTATGGGCACCAAACCCTCTGTAGAAGCCATCGGAATCGGAGGTTTCAGATCTCACCCCCTTTCTTTCTTCTATTCAATTCAATTCAATTCAACTTTTATTCCTCTTCACCCCTTTCTTTCTTCTATTCAATTCNACTTTTA
TARGET=4,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03444 Ps_contig_mira-and-tgicl-ass_franssen_30006
SEQUENCE=AACATTTATTTATTTTGGTTATGGCAAAAAATAGTTAATAACTAATCAAAAGAAAAAACTTTACTACACCCAGTATCGANATTTTATTAGTTGAAGTTTGTATTAAAATTTTAATGGTTCTCAGATTTTCTTTTAATACCTGAATGCCATATCATTCCCATATTGTTCTTCATCAAACATTGTCATACCATTCACATATTGTTCTTCATCAAACAATGTGCTCCTATAAGGATTTCTTTTTTTGCCAGTAAAACGGTCGACTTCATACAGCCCAGCCCTTTACAGAAATCAATTGAACACGTCTCAATCTTGGCATGAGTAATTGAAACTTTTGTCTTTAATAAGAATAAAATTACTAGCATATCAATCGGTTTCTACAGCATTTTTGTATCAGTCCTCCAACTGCACAGGCTCTGTGACAGGAGGTATACAATCTACCTTATACCGTAGCACCTTTCTAAAAAAAGTTGAATGTGTGTTGTCCCATATCAGTTTATAGCTTCCAGCCATTAATGTGCAGAAGTTACCTTGGTCAGACTCGTATCGCCGGTGAGGCAACATCAGAGTCTTTTCCCCAGTAGGACTTACAAACTCCAAACTAAATCCAATATCCATATTTATCTTGCCTTGTACTAGAGAAAAGTCCCATGCAATATACGAGTTTACTGAGTCCACTGAGAGTATAACCTCAAAAGTTTTCCCAGCTGGAACACTTGCCTCTTTGAAGTCATCAATTTTACTTACTTCCACTGCACCGGCAACATCTGCTGGAATGAAGAAAGGCGAGTTTGCAAAGACCCCACTAATAAATGTGATGAACCTCTCAAACTCCATGGTGTAGACATCAACAGCTTGTTCCAAGACAGCAAACTGTTCCCTTTTTAATTTCTTATACTCATATCGTGCCCACGATAATGAAAGGAAGAACCGGACATAAAGAATCATTGAGGTGAGGCTGTATAAAGGTGGTCCATAGCGATGACGTTGCTCCTCCATGGGCCCGTCATCGTCATTGATCTGCCTTGTAAAATCAAGCAATACCTCAGGAACTCCAACTAGGTACTTCACGAGGGTTTTATAGACTCCTGTTGCAGAGCCAAGCTGAGCTAATTGCCTTCTCCTGTCCATTTGTTGTTTCCACAGAAGAGCATATCGATCATGTATGCTACCACCAGAATTCACCAAAGCATCTACCTCTGCTTGTTTATTCCTCTCTGAGCGTTCCCGTTGTTGTCTGAGGAGAGTTCCTCGAAAATCTTGAGGCAGATATGTCATAACTGTGTTAAATACATGTTCAGCATTTTTTATTTGGAAAGATTCCAAAGTCCTCAAAGTATTATGAAGTTTATCTTTGAGCTTGGGAAGCATAATGGAAAATTCAACATTGTCAGGAGCGGGCGGGTGTGGGCAATTAGAAGGTTCAAGTAAAAAAATAGTTTCTTCAATGTATTCTCGATTCTTCCACATATTTTCATCAATCTTATGCGGAGGTTGGGTCTGAGCTTCATTTATTAACGTGGTTTCTCCATGGATAGGAGGATGTTGAGTGAGCAAATCAGTGGCCATAGAACGAAGCTCGGAAGAAAGGCGAGAGACATCTGGAGAAAGAGGAGTAAAGGGGTGTTGATCGTAGTAAGACGCCAGAAAGGCCCTAGTTATGGGCACCAAACCCTCTGTAGAAGCCATCGGAATCGGAGGTTTCAGATCTCACCCCCTTTCTTTCTTCTATTCAATTCAATTCAATTCAACTTTTATTCCTCTTCACCCCTTTCTTTCTTCTATTCAATTCNACTTTTA
TARGET=1728,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1975
PRIMER_LEFT_PENALTY=0.095538
PRIMER_RIGHT_PENALTY=0.102004
PRIMER_LEFT_SEQUENCE=TGATCGTAGTAAGACGCCAGAA
PRIMER_RIGHT_SEQUENCE=AAGAAAGAAAGGGGTGAAGAGG
PRIMER_LEFT=1624,22
PRIMER_RIGHT=1777,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.1975
PRIMER_LEFT_1_PENALTY=0.095538
PRIMER_RIGHT_1_PENALTY=0.102004
PRIMER_LEFT_1_SEQUENCE=TTGATCGTAGTAAGACGCCAGA
PRIMER_RIGHT_1_SEQUENCE=AAGAAAGAAAGGGGTGAAGAGG
PRIMER_LEFT_1=1623,22
PRIMER_RIGHT_1=1777,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=155
PRIMER_PAIR_PENALTY_2=0.2267
PRIMER_LEFT_2_PENALTY=0.124655
PRIMER_RIGHT_2_PENALTY=0.102004
PRIMER_LEFT_2_SEQUENCE=TAGGAGGATGTTGAGTGAGCAA
PRIMER_RIGHT_2_SEQUENCE=AAGAAAGAAAGGGGTGAAGAGG
PRIMER_LEFT_2=1532,22
PRIMER_RIGHT_2=1777,22
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=0.2324
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.102004
PRIMER_LEFT_3_SEQUENCE=CAAACCCTCTGTAGAAGCCATC
PRIMER_RIGHT_3_SEQUENCE=AAGAAAGAAAGGGGTGAAGAGG
PRIMER_LEFT_3=1665,22
PRIMER_RIGHT_3=1777,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=0.2443
PRIMER_LEFT_4_PENALTY=0.142283
PRIMER_RIGHT_4_PENALTY=0.102004
PRIMER_LEFT_4_SEQUENCE=TAGAACGAAGCTCGGAAGAAAG
PRIMER_RIGHT_4_SEQUENCE=AAGAAAGAAAGGGGTGAAGAGG
PRIMER_LEFT_4=1565,22
PRIMER_RIGHT_4=1777,22
PRIMER_LEFT_4_TM=60.142
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_03445 Ps_contig_mira-and-tgicl-ass_franssen_30009
SEQUENCE=CGGGGAATTTTATTCATTTTTATTTTCTTCATTTTGAGAGAGAGAGAGAAAGAAGAGAAAGAAGTCGAAGAAGAAGAAGAATCCAAAAGCAATCCCATCTCTTCTGCGTGTCGTTGCACATCAGAACTGAAGGGAGAGAGAGAAACCCATCGGTTTCACAAATTAATGAAGAATTTCACACCGACACTATCGATTTCCACCTCTTAAAGGGTTGCTTTGGAATCGGGTTACGTCTTCTTCGTTTCTCTTCGATTTCCTTTGTGGGAGATCTAATTTGGTTAGGTTTTTTGGGGTGTTTCGGAAGTTACATGCATATGGGAGCAGTGGTTGTGTTGGATGCTATAAAAGGCCCGTACTAGTTACTACTGTGGAAGCACCGTCGAAAGGGGTTATAATAGTAGGCAAGCCAGTGGATAAACCTAGTACATCGGAGGATTTCTGGACCACCAGCACGCATGATATGGACAACAGTGCTGTTCAATCACAAGGAAGTATATCTTCAACCAGTCTAACTAACCAAGCTGTCGTTCCTCATGGCAGTTCTTCCAACAACCCGCCTGAGTTTGTAAATCATGGTCTAATTCTATGGAATCAGACTCGACAGCGTTGGGTTGGAAATAAAAAGTCCGAGAATAAAACACAACAATTGCGAGAACCTAAATTGAGTTGGAATGCAACATACGAAAGTTTACTGACAAACAACAAGCCATTCCGTCAGCCCATCCCCCTTGCTGAAATGGTAGATTTTCTTGTGGACGTTTGGGAACAGGATGGCCTGTATGACTGAGCCGATGAAGGTTGAGGAAGTGCGGTTTCGTTTGTTTGATCCTTGAAATAATTTTAGATCTTGCTTCTTTAGAAACAGGAGGAAGGGGACTGAATGAATATGTTGCAGAGTTTGTATATCAATTGTTATATGCCAAAGAGTGTAATTTCTCTCAGCAGGCGTTAAGATGATTGTATTCTACTTTTATTTAATTGATTGCCGTTTCCTCTCTGGTATCCATAAGAGGCAAAGTTTTTAAGAGCTCCTTTTGTTGACTGCATGGTTGCTGCACTTCCATTTCCTGTTGTTTTTCTGTCAAATTTGACCAAAAATATGTAGGAAATGGNTTTCTGACTTGGTTTTCATA
TARGET=36,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03446 Ps_contig_mira-and-tgicl-ass_franssen_30009
SEQUENCE=CGGGGAATTTTATTCATTTTTATTTTCTTCATTTTGAGAGAGAGAGAGAAAGAAGAGAAAGAAGTCGAAGAAGAAGAAGAATCCAAAAGCAATCCCATCTCTTCTGCGTGTCGTTGCACATCAGAACTGAAGGGAGAGAGAGAAACCCATCGGTTTCACAAATTAATGAAGAATTTCACACCGACACTATCGATTTCCACCTCTTAAAGGGTTGCTTTGGAATCGGGTTACGTCTTCTTCGTTTCTCTTCGATTTCCTTTGTGGGAGATCTAATTTGGTTAGGTTTTTTGGGGTGTTTCGGAAGTTACATGCATATGGGAGCAGTGGTTGTGTTGGATGCTATAAAAGGCCCGTACTAGTTACTACTGTGGAAGCACCGTCGAAAGGGGTTATAATAGTAGGCAAGCCAGTGGATAAACCTAGTACATCGGAGGATTTCTGGACCACCAGCACGCATGATATGGACAACAGTGCTGTTCAATCACAAGGAAGTATATCTTCAACCAGTCTAACTAACCAAGCTGTCGTTCCTCATGGCAGTTCTTCCAACAACCCGCCTGAGTTTGTAAATCATGGTCTAATTCTATGGAATCAGACTCGACAGCGTTGGGTTGGAAATAAAAAGTCCGAGAATAAAACACAACAATTGCGAGAACCTAAATTGAGTTGGAATGCAACATACGAAAGTTTACTGACAAACAACAAGCCATTCCGTCAGCCCATCCCCCTTGCTGAAATGGTAGATTTTCTTGTGGACGTTTGGGAACAGGATGGCCTGTATGACTGAGCCGATGAAGGTTGAGGAAGTGCGGTTTCGTTTGTTTGATCCTTGAAATAATTTTAGATCTTGCTTCTTTAGAAACAGGAGGAAGGGGACTGAATGAATATGTTGCAGAGTTTGTATATCAATTGTTATATGCCAAAGAGTGTAATTTCTCTCAGCAGGCGTTAAGATGATTGTATTCTACTTTTATTTAATTGATTGCCGTTTCCTCTCTGGTATCCATAAGAGGCAAAGTTTTTAAGAGCTCCTTTTGTTGACTGCATGGTTGCTGCACTTCCATTTCCTGTTGTTTTTCTGTCAAATTTGACCAAAAATATGTAGGAAATGGNTTTCTGACTTGGTTTTCATA
TARGET=67,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.6650
PRIMER_LEFT_PENALTY=4.664988
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=GAGAGAAAGAAGAGAAAGAAGTCG
PRIMER_RIGHT_SEQUENCE=GAAATCGATAGTGTCGGTGTGA
PRIMER_LEFT=43,24
PRIMER_RIGHT=196,22
PRIMER_LEFT_TM=57.335
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=4.7312
PRIMER_LEFT_1_PENALTY=4.731137
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=AGAGAGAAAGAAGAGAAAGAAGTCG
PRIMER_RIGHT_1_SEQUENCE=GAAATCGATAGTGTCGGTGTGA
PRIMER_LEFT_1=42,25
PRIMER_RIGHT_1=196,22
PRIMER_LEFT_1_TM=58.269
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=155
PRIMER_PAIR_PENALTY_2=4.7447
PRIMER_LEFT_2_PENALTY=4.664988
PRIMER_RIGHT_2_PENALTY=0.079714
PRIMER_LEFT_2_SEQUENCE=GAGAGAAAGAAGAGAAAGAAGTCG
PRIMER_RIGHT_2_SEQUENCE=ATAGCATCCAACACAACCACTG
PRIMER_LEFT_2=43,24
PRIMER_RIGHT_2=342,22
PRIMER_LEFT_2_TM=57.335
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=4.8296
PRIMER_LEFT_3_PENALTY=4.664988
PRIMER_RIGHT_3_PENALTY=0.164639
PRIMER_LEFT_3_SEQUENCE=GAGAGAAAGAAGAGAAAGAAGTCG
PRIMER_RIGHT_3_SEQUENCE=GGAAATCGAAGAGAAACGAAGA
PRIMER_LEFT_3=43,24
PRIMER_RIGHT_3=256,22
PRIMER_LEFT_3_TM=57.335
PRIMER_RIGHT_3_TM=59.835
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=4.8945
PRIMER_LEFT_4_PENALTY=4.664988
PRIMER_RIGHT_4_PENALTY=0.229480
PRIMER_LEFT_4_SEQUENCE=GAGAGAAAGAAGAGAAAGAAGTCG
PRIMER_RIGHT_4_SEQUENCE=CAAAGGAAATCGAAGAGAAACG
PRIMER_LEFT_4=43,24
PRIMER_RIGHT_4=260,22
PRIMER_LEFT_4_TM=57.335
PRIMER_RIGHT_4_TM=60.229
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_03447 Ps_contig_mira-and-tgicl-ass_franssen_30009
SEQUENCE=CGGGGAATTTTATTCATTTTTATTTTCTTCATTTTGAGAGAGAGAGAGAAAGAAGAGAAAGAAGTCGAAGAAGAAGAAGAATCCAAAAGCAATCCCATCTCTTCTGCGTGTCGTTGCACATCAGAACTGAAGGGAGAGAGAGAAACCCATCGGTTTCACAAATTAATGAAGAATTTCACACCGACACTATCGATTTCCACCTCTTAAAGGGTTGCTTTGGAATCGGGTTACGTCTTCTTCGTTTCTCTTCGATTTCCTTTGTGGGAGATCTAATTTGGTTAGGTTTTTTGGGGTGTTTCGGAAGTTACATGCATATGGGAGCAGTGGTTGTGTTGGATGCTATAAAAGGCCCGTACTAGTTACTACTGTGGAAGCACCGTCGAAAGGGGTTATAATAGTAGGCAAGCCAGTGGATAAACCTAGTACATCGGAGGATTTCTGGACCACCAGCACGCATGATATGGACAACAGTGCTGTTCAATCACAAGGAAGTATATCTTCAACCAGTCTAACTAACCAAGCTGTCGTTCCTCATGGCAGTTCTTCCAACAACCCGCCTGAGTTTGTAAATCATGGTCTAATTCTATGGAATCAGACTCGACAGCGTTGGGTTGGAAATAAAAAGTCCGAGAATAAAACACAACAATTGCGAGAACCTAAATTGAGTTGGAATGCAACATACGAAAGTTTACTGACAAACAACAAGCCATTCCGTCAGCCCATCCCCCTTGCTGAAATGGTAGATTTTCTTGTGGACGTTTGGGAACAGGATGGCCTGTATGACTGAGCCGATGAAGGTTGAGGAAGTGCGGTTTCGTTTGTTTGATCCTTGAAATAATTTTAGATCTTGCTTCTTTAGAAACAGGAGGAAGGGGACTGAATGAATATGTTGCAGAGTTTGTATATCAATTGTTATATGCCAAAGAGTGTAATTTCTCTCAGCAGGCGTTAAGATGATTGTATTCTACTTTTATTTAATTGATTGCCGTTTCCTCTCTGGTATCCATAAGAGGCAAAGTTTTTAAGAGCTCCTTTTGTTGACTGCATGGTTGCTGCACTTCCATTTCCTGTTGTTTTTCTGTCAAATTTGACCAAAAATATGTAGGAAATGGNTTTCTGACTTGGTTTTCATA
TARGET=134,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2747
PRIMER_LEFT_PENALTY=0.274705
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=AAAAGCAATCCCATCTCTTCTG
PRIMER_RIGHT_SEQUENCE=GAAATCGATAGTGTCGGTGTGA
PRIMER_LEFT=84,22
PRIMER_RIGHT=196,22
PRIMER_LEFT_TM=59.725
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=113
PRIMER_PAIR_PENALTY_1=0.2747
PRIMER_LEFT_1_PENALTY=0.274705
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=CAAAAGCAATCCCATCTCTTCT
PRIMER_RIGHT_1_SEQUENCE=GAAATCGATAGTGTCGGTGTGA
PRIMER_LEFT_1=83,22
PRIMER_RIGHT_1=196,22
PRIMER_LEFT_1_TM=59.725
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=0.3544
PRIMER_LEFT_2_PENALTY=0.274705
PRIMER_RIGHT_2_PENALTY=0.079714
PRIMER_LEFT_2_SEQUENCE=AAAAGCAATCCCATCTCTTCTG
PRIMER_RIGHT_2_SEQUENCE=ATAGCATCCAACACAACCACTG
PRIMER_LEFT_2=84,22
PRIMER_RIGHT_2=342,22
PRIMER_LEFT_2_TM=59.725
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.3544
PRIMER_LEFT_3_PENALTY=0.274705
PRIMER_RIGHT_3_PENALTY=0.079714
PRIMER_LEFT_3_SEQUENCE=CAAAAGCAATCCCATCTCTTCT
PRIMER_RIGHT_3_SEQUENCE=ATAGCATCCAACACAACCACTG
PRIMER_LEFT_3=83,22
PRIMER_RIGHT_3=342,22
PRIMER_LEFT_3_TM=59.725
PRIMER_RIGHT_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.4049
PRIMER_LEFT_4_PENALTY=0.274705
PRIMER_RIGHT_4_PENALTY=0.130244
PRIMER_LEFT_4_SEQUENCE=AAAAGCAATCCCATCTCTTCTG
PRIMER_RIGHT_4_SEQUENCE=GGCCTTTTATAGCATCCAACAC
PRIMER_LEFT_4=84,22
PRIMER_RIGHT_4=350,22
PRIMER_LEFT_4_TM=59.725
PRIMER_RIGHT_4_TM=59.870
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_03448 Ps_contig_mira-and-tgicl-ass_franssen_30017
SEQUENCE=AAAAAAGAAATGAGTCTAAAGAGGATTCCAAGTCAAACACAGAGGATCAAGGTGGTTTTCAAGAAGCCTTCATGAAGCAATTTCAAAGTTTTCTCACCCCATTGTTGGTGATGGGGCTATTTCTGTCATCCTTTTCTTTTGGTCCTCGTGAGCAGCAGCAGATTAGCTTTCAGGAGTTTAAAAATAAGCTTTTGGAACCAGGGTTAGTAGACCATATTGTTGTCTCCAACAAATCAGTTGCCAAAATATATGTGAGAAACTCTCCCCGTGATCAAGCTGACAGCGAAGTACTACAAGGAAACCTTCCTGCAAAGGGGTCTAGTGGCCATTATAAATATTATTTCAATATTGGAAGTGTCGAGTCTTTTGAGGAGAAGTTAGAGGAAGTGCAAGAAACACTAGGTGTTGACCCTCACGATTATGTACCTGTTACATATTCTTCTGAACTGGTTTGGTACCAGGAGTTGATGAGGTTTGCTCCAACCCTGTTACTTTTGGGAACTCTCTTGTATATGGGAAGGAGAATGCAAGGTGGATTTGGTGTTGGCGGCGGCGGCGGTGGTAAGGGTGCTCGTGGAATATTTAACATTGGAAAAGCACATGTTACTAAAGTAGACAAAAATGCCAAAAACAAGGTCTATTTTAAAGATGTTGCTGGCTGTGATGAGGCAAAACAAGAAATTATGGAGTTTGTGCACTTCCTCAAGAATCCTAAGAAGTACGAAGAGCTTGGTGCCAAAATTCCAAAAGGTGCTCTTCTGGTCGGTCCCCCAGGCACAGGAAAAACCCTTTTAGCAAAAGCAACTGCAGGGGAGTCTGGTGTGCCATTTCTTTCTATATCTGGTTCCGATTTCATGGAAATGTTTGTCGGTGTTGGACCGTCAAGGGTGAGAAACTTGTTTCAGGAAGCAAGGCAGTGTGCTCCCAGTATAATATTTATTGATGAGATTGATGCAATTGGTCGGAAAAGGGGCCGTGGAGGTTTCTCTGGTTCAAATGATGAGCGTGAAAGTACTTTAAATCAATTGTTGGTGGAGATGGATGGTTTTGGAACCACTGCTGGAGTTGTTGTGCTAGCAGGTACAAATAGACCCGATATATTAGACAATGCCCTACTTAGGCCTGGGCGTTTTGACCGCCAGATAACAATTGATGTACCCGACATTAAAGGCCGTGACCAGATTTTTCAGATTTACTTGAAAACGATCAAACTTGACCACGAGCCCTCATATTATTCTCAAAGGCTTGCAGCCCTTACTCCAGGTTTTGCTGGAGCAGACATTGCTAATGTTTGCAATGAAGCTGCGCTGATTGCTGCAAGAACTGATGAAGCACAAGTCACAATGGATCATTTCGAGGCAGCTATTGATAGAATCATTGGTGGTTTGGAGAAGAAGAACAAGGTAATAAGTAAGCTGGAAAGGCGTACTGTTGCTTACCATGAAGCAGGTCATGCTGTTACAGGTTGGTTCTTGGAACATACTGATCCATTGTTGAAAGTAACTATTGTTCCTCGCGGAACAGCAGCTCTTGGGTTTGCGCAGTATGTTCCTAATGAGAATCTCCTCAAGACTAAGGAGCAGCTTTTCGATATGACTTGTATGACCCTTGGTGGCCGGGCTGCCGAGAAGGTTCTCATTGGGACAATCTCTACCGGAGCACAAAATGATTTAGAGAAAGTGACCAAGATGACTTATGCCCAAGTAGCCGTCTATGGTTTCAGCGAAAAAGTGGGCCTCCTCTCTTTCCCTCAAAGGGAGGACTCAATGGAGATGACCAAACCATACAGCAGCAAAACTGGTGCAATAATTGATACTGAAGTCCGAGAATGGGTGAACAAAGCATATGAACGTACCATACAGCTTATAGAGGAACACAAGGCGAAAGTAGCCGAAATTGCAGAGTTGTTGCTTGAAAAAGAAGTACTTCACCAAGAAGACTTGCTTCGAGTCTTGGGTGAGCGGCCCTTCCAAAGTGCTGAGCCCACTCATTATGATAGATTTAAGCTAGGGTTTCAAGATGAGGAGAAAGTTGTTGAAACCACAGTAAATGAAGCCAAGGATGATGGTGGGTCTTCACCTCTAGAGCCAGAGGTTGTTCCCACATAGTTGAGCAAGTTTATGTTTGTTACCATTGTACATGAAATTGACAATCTTATTCCGCCATTTTGCTGATGTGGACAAAACTTGTATGATAATTGCATAATAAAAATATTGTTCTTGANTAAGT
TARGET=546,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1167
PRIMER_LEFT_PENALTY=0.035021
PRIMER_RIGHT_PENALTY=0.081670
PRIMER_LEFT_SEQUENCE=TTGCTCCAACCCTGTTACTTTT
PRIMER_RIGHT_SEQUENCE=GCACCAAGCTCTTCGTACTTCT
PRIMER_LEFT=474,22
PRIMER_RIGHT=735,22
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=0.1167
PRIMER_LEFT_1_PENALTY=0.035021
PRIMER_RIGHT_1_PENALTY=0.081670
PRIMER_LEFT_1_SEQUENCE=TTTGCTCCAACCCTGTTACTTT
PRIMER_RIGHT_1_SEQUENCE=GCACCAAGCTCTTCGTACTTCT
PRIMER_LEFT_1=473,22
PRIMER_RIGHT_1=735,22
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=60.082
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.1711
PRIMER_LEFT_2_PENALTY=0.035021
PRIMER_RIGHT_2_PENALTY=0.136083
PRIMER_LEFT_2_SEQUENCE=TTGCTCCAACCCTGTTACTTTT
PRIMER_RIGHT_2_SEQUENCE=TAAATATTCCACGAGCACCCTT
PRIMER_LEFT_2=474,22
PRIMER_RIGHT_2=584,22
PRIMER_LEFT_2_TM=60.035
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=0.1711
PRIMER_LEFT_3_PENALTY=0.035021
PRIMER_RIGHT_3_PENALTY=0.136083
PRIMER_LEFT_3_SEQUENCE=TTGCTCCAACCCTGTTACTTTT
PRIMER_RIGHT_3_SEQUENCE=TTAAATATTCCACGAGCACCCT
PRIMER_LEFT_3=474,22
PRIMER_RIGHT_3=585,22
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=112
PRIMER_PAIR_PENALTY_4=0.1711
PRIMER_LEFT_4_PENALTY=0.035021
PRIMER_RIGHT_4_PENALTY=0.136083
PRIMER_LEFT_4_SEQUENCE=TTTGCTCCAACCCTGTTACTTT
PRIMER_RIGHT_4_SEQUENCE=TAAATATTCCACGAGCACCCTT
PRIMER_LEFT_4=473,22
PRIMER_RIGHT_4=584,22
PRIMER_LEFT_4_TM=60.035
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=112
=
PRIMER_SEQUENCE_ID=SSR_03449 Ps_contig_mira-and-tgicl-ass_franssen_30024
SEQUENCE=GGGGACACCAAAAAATGAAGTGATAAGAACTACACTAAAAATATGAGGTCCAAATCTCACATCAATTCGGATAAGAAAAGAATCTTAAAACCCCAGGGGTATTGGTCCTCATTTCTCCATCTTAACTTCACCAAATATAATTCCAAACCAAAAATTTCTCCTAACCTGCAAAATCCACACATCATTCACACTCTCAAAACAAGCACTTTCACTCATTCATCAATCAATCAATGGCATCAACCATAATCGCAATGACCATGATGAACACAAAGTGCTTCAACACTCCTAGTTCACCAAAGTTCCACAACCCAACAAAACTCGTTACAAAACCCACTTCACTCCTTTCCCTCAAGAACCTTCTTCCAAAAGGAACTCCCGCAACAGAAAGCACCAAACTATCAACTTCCATAGCAGGAACAGCACTCGCAGGAGCAATATTCTCAACCCTCGGAGCCTGTAACCCCGCTTTTGCAGCACAACAAATAGCTGACATAGCTGAAGCATCATCAGATAACCGTGGTTTAGCACTTCTGTTACCTATAATTCCAGCCATAGCTTGGGTTCTGTTCAACATTCTTCAACCAGCACTTAACCAAATCAACCGTATGCGTAACACGAAAGGGGTTATCATTGGTTTGGGTCTTGGTGGATTAACAGCTTCAGGGTTTGTGTCAACACAAGATGCAATGGCTAGTGAAATTGCTGCTATTGCTGAAGCTGGTAGTGATAATAGGGGTCAGCTTCTGTTGTTTGTTGTAGCACCTGCCATTCTTTGGGTTCTCTATAATATTTTGCAGCCTGCTCTTAATCAAATCAACAAAATGAGATCAGATTGAAACTTTTCCTTACATATATAAAATAAAAAGCAGTTTTTGTTTTCTTTTGTTGTTTCTGTTGTTGGATATATATATCATTCAATTCTGTGTAACTGTATCTAATGTTATATCCGTTTTTTTCAATGCCTTGTGTTTTCTGTTTTATTTATTAACTTCCTTTTGTTCTGTTATAAACTGTGATTTCTTGTTGTTGCAACTGTTTTAAACCATTCACAGTTCACTTATGAAGAATGTGTTTGGATTTGNCAAAAAANNAGCTATATATGTGGTTTAAGTTT
TARGET=219,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0720
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TTGGTCCTCATTTCTCCATCTT
PRIMER_RIGHT_SEQUENCE=GAAAGGAGTGAAGTGGGTTTTG
PRIMER_LEFT=101,22
PRIMER_RIGHT=345,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=245
PRIMER_PAIR_PENALTY_1=0.0874
PRIMER_LEFT_1_PENALTY=0.075241
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=AGGGGTATTGGTCCTCATTTCT
PRIMER_RIGHT_1_SEQUENCE=GAAAGGAGTGAAGTGGGTTTTG
PRIMER_LEFT_1=94,22
PRIMER_RIGHT_1=345,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.0972
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.037339
PRIMER_LEFT_2_SEQUENCE=TTGGTCCTCATTTCTCCATCTT
PRIMER_RIGHT_2_SEQUENCE=AAGCACTTTGTGTTCATCATGG
PRIMER_LEFT_2=101,22
PRIMER_RIGHT_2=276,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=176
PRIMER_PAIR_PENALTY_3=0.1126
PRIMER_LEFT_3_PENALTY=0.075241
PRIMER_RIGHT_3_PENALTY=0.037339
PRIMER_LEFT_3_SEQUENCE=AGGGGTATTGGTCCTCATTTCT
PRIMER_RIGHT_3_SEQUENCE=AAGCACTTTGTGTTCATCATGG
PRIMER_LEFT_3=94,22
PRIMER_RIGHT_3=276,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=60.037
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.1252
PRIMER_LEFT_4_PENALTY=0.059875
PRIMER_RIGHT_4_PENALTY=0.065292
PRIMER_LEFT_4_SEQUENCE=TTGGTCCTCATTTCTCCATCTT
PRIMER_RIGHT_4_SEQUENCE=TAACGAGTTTTGTTGGGTTGTG
PRIMER_LEFT_4=101,22
PRIMER_RIGHT_4=323,22
PRIMER_LEFT_4_TM=59.940
PRIMER_RIGHT_4_TM=59.935
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_03450 Ps_contig_mira-and-tgicl-ass_franssen_30024
SEQUENCE=GGGGACACCAAAAAATGAAGTGATAAGAACTACACTAAAAATATGAGGTCCAAATCTCACATCAATTCGGATAAGAAAAGAATCTTAAAACCCCAGGGGTATTGGTCCTCATTTCTCCATCTTAACTTCACCAAATATAATTCCAAACCAAAAATTTCTCCTAACCTGCAAAATCCACACATCATTCACACTCTCAAAACAAGCACTTTCACTCATTCATCAATCAATCAATGGCATCAACCATAATCGCAATGACCATGATGAACACAAAGTGCTTCAACACTCCTAGTTCACCAAAGTTCCACAACCCAACAAAACTCGTTACAAAACCCACTTCACTCCTTTCCCTCAAGAACCTTCTTCCAAAAGGAACTCCCGCAACAGAAAGCACCAAACTATCAACTTCCATAGCAGGAACAGCACTCGCAGGAGCAATATTCTCAACCCTCGGAGCCTGTAACCCCGCTTTTGCAGCACAACAAATAGCTGACATAGCTGAAGCATCATCAGATAACCGTGGTTTAGCACTTCTGTTACCTATAATTCCAGCCATAGCTTGGGTTCTGTTCAACATTCTTCAACCAGCACTTAACCAAATCAACCGTATGCGTAACACGAAAGGGGTTATCATTGGTTTGGGTCTTGGTGGATTAACAGCTTCAGGGTTTGTGTCAACACAAGATGCAATGGCTAGTGAAATTGCTGCTATTGCTGAAGCTGGTAGTGATAATAGGGGTCAGCTTCTGTTGTTTGTTGTAGCACCTGCCATTCTTTGGGTTCTCTATAATATTTTGCAGCCTGCTCTTAATCAAATCAACAAAATGAGATCAGATTGAAACTTTTCCTTACATATATAAAATAAAAAGCAGTTTTTGTTTTCTTTTGTTGTTTCTGTTGTTGGATATATATATCATTCAATTCTGTGTAACTGTATCTAATGTTATATCCGTTTTTTTCAATGCCTTGTGTTTTCTGTTTTATTTATTAACTTCCTTTTGTTCTGTTATAAACTGTGATTTCTTGTTGTTGCAACTGTTTTAAACCATTCACAGTTCACTTATGAAGAATGTGTTTGGATTTGNCAAAAAANNAGCTATATATGTGGTTTAAGTTT
TARGET=902,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03451 Ps_contig_mira-and-tgicl-ass_franssen_30032
SEQUENCE=CGGTTTTATTTATTTATTAAACAAAATTTCCTGAATGCTACTAATAAATGCTTAAATACAAGCTGTGGTTAAATTCAACAGAAAGATTAATCTGATATTTGGGATCCCACTTTCACTTATGATTTTCATGTTTCTATTCAGAGATAGAATCACAAATACTTTAGATACATTTAATGTAACATTCTCTAGCTACAAAATGGAAGAGAGAAATGTTGAACTAAAATGATCACTAATCATCACAACATGTTGTGAGCAGGTAAATCTTTTATACTGGAGATGTCTTCAAGAGCAAGCTCCCGCTCTAACTGCGTTCTTGTTGACTTCAAAACATCGTTAAATTCCATATATGTAGCACGCTTTGCTAGCCACTGAGCATCCATCATTTGAAAGGCGATGCAGAAAAGGATATCAAATGCCATTTCATCCTCCTCAAGCAGCCGTAGAAAATAGATTCCTGGCAAAGAAGATGGATGAACTGATTGAAGATCCAGCATCTGCACCAACATAAATGAAATATTGATTCCAGCTACTGCAAATGGATATTCCCACTCTGCTCTGACTCCATCTTGTTTAAGCAACAATCGCTGGAATGAAGCTGGATACTTCTGCGCGAAAAAGATGAGGTTCTCTAATGATATAAATCCTCCACCCCTAAATCTGTTGATGGGTCTGAACCTTGCCACTCCCTATTTTCCCTTACCAAAACATCTAGTAATTTAAGGTAGGAA
TARGET=5,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03452 Ps_contig_mira-and-tgicl-ass_franssen_30034
SEQUENCE=CGTTAATTCAATTTCATAATCATATAAAAGATTAATCAATAATATTAACCTATTCCCAGATTCACAAATTCAAACAGAAGAATTATAGAATTTAGATGAAAGTAACAAGGAAAAAAAAAATAGCCAATAATGTTCAGCTATACTGATACTATATATCTTATGCATCAACAACTAATTATTTATACAAATTACATGAGAAAATAGAAAATGTCTTTTTTTCAAAATTAATGAAATCTCATTCTCTCTCATTTCATACTCTGTTTTTCCAACACTATAGCTATTTTTCATTTTTCTTCATTCTTCATTTCATTTCTAAGCCTTTGTAATCCCAAACACCAGCTTTCAAACCCAAGAAATCATAAGCAGGTGCTTTTCCGTCGCTGGAATTTCCAACAATCTCGTCACAGCTACAATCTTTACTGTTCTGTAAACCCAAACTACAAACAAAACAGAGACTATTGTTGTTGTTGCTATTACTGTTGTTGTTGTTTCTGTCTCTGTTTTTGAATTGTTCTTCAGATTCTTGTTGCCGTGGAGGACTTATAGTTAACTCAAGATTCAAATCCGGACACCGCTCTAACACCGACCCTTTAATCATCATCTCTTGATGATGATTATGATAATGATTATGATATTGTTCTTGTTTAATAGACGGAGCGAAAGAGATAATTGATGTGGGTGTGGTAGCTGCTATAACAGTAGAAGTAGTAGTTGATACAGATGCAGCAGCAGCTACAGCTATAGTTACACCTTCTTGATTCTGGTTCTGGTTCTGGTTCTGAAGATGAAGAACTTGAGAATGAGTTTGAGATTGAGATTGAGATTGAGAATGAGAATGAGAAACCTCGTTTAAAGGTCTATGAGTAGCAGGGTCAATTCCTCTATTCAAAAGCTTTCTTCTAATATGAGTATTCCAATAATTCTTTATCTCATTATCTGTTCTACCGGGTAATCTTCCAGCTATCAAAGACCATTTGTTACCAAGAAGACTATGAAGTTTGATAATAAGTTCATCTTCTTCTTCGGTAAAGTTCCCGCGTTTAAGGTCCGGTCGGAGATAATTAATCCAGCGGAGACGGCAGCTTTTGCCGCAGCGGAGTAAACCAGCAGCTTTAGGAAGAGATCTCCAACAACCTTCACCGTGAGCACGAATATAAGAAATCAATCTATCGTCTTCTTCTTTAGTCCAAGCACCTTTGTTAGTATGAGCTTTTTCACAACAGGGTGATCTTCCCATGATTTGTTATGGAAAAACTAGAAAACAAGAAGAAAACAGAAAGCCCTCTGCGTTGAATACCACTGCTT
TARGET=459,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1301
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=CAGCTTTCAAACCCAAGAAATC
PRIMER_RIGHT_SEQUENCE=AACTATAAGTCCTCCACGGCAA
PRIMER_LEFT=336,22
PRIMER_RIGHT=547,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.1438
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=GTAATCCCAAACACCAGCTTTC
PRIMER_RIGHT_1_SEQUENCE=AACTATAAGTCCTCCACGGCAA
PRIMER_LEFT_1=322,22
PRIMER_RIGHT_1=547,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.2183
PRIMER_LEFT_2_PENALTY=0.197140
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=CCAACAATCTCGTCACAGCTAC
PRIMER_RIGHT_2_SEQUENCE=AACTATAAGTCCTCCACGGCAA
PRIMER_LEFT_2=389,22
PRIMER_RIGHT_2=547,22
PRIMER_LEFT_2_TM=59.803
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.3102
PRIMER_LEFT_3_PENALTY=0.108929
PRIMER_RIGHT_3_PENALTY=0.201288
PRIMER_LEFT_3_SEQUENCE=CAGCTTTCAAACCCAAGAAATC
PRIMER_RIGHT_3_SEQUENCE=GAGATGATGATTAAAGGGTCGG
PRIMER_LEFT_3=336,22
PRIMER_RIGHT_3=603,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.799
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.3239
PRIMER_LEFT_4_PENALTY=0.122569
PRIMER_RIGHT_4_PENALTY=0.201288
PRIMER_LEFT_4_SEQUENCE=GTAATCCCAAACACCAGCTTTC
PRIMER_RIGHT_4_SEQUENCE=GAGATGATGATTAAAGGGTCGG
PRIMER_LEFT_4=322,22
PRIMER_RIGHT_4=603,22
PRIMER_LEFT_4_TM=59.877
PRIMER_RIGHT_4_TM=59.799
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_03453 Ps_contig_mira-and-tgicl-ass_franssen_30034
SEQUENCE=CGTTAATTCAATTTCATAATCATATAAAAGATTAATCAATAATATTAACCTATTCCCAGATTCACAAATTCAAACAGAAGAATTATAGAATTTAGATGAAAGTAACAAGGAAAAAAAAAATAGCCAATAATGTTCAGCTATACTGATACTATATATCTTATGCATCAACAACTAATTATTTATACAAATTACATGAGAAAATAGAAAATGTCTTTTTTTCAAAATTAATGAAATCTCATTCTCTCTCATTTCATACTCTGTTTTTCCAACACTATAGCTATTTTTCATTTTTCTTCATTCTTCATTTCATTTCTAAGCCTTTGTAATCCCAAACACCAGCTTTCAAACCCAAGAAATCATAAGCAGGTGCTTTTCCGTCGCTGGAATTTCCAACAATCTCGTCACAGCTACAATCTTTACTGTTCTGTAAACCCAAACTACAAACAAAACAGAGACTATTGTTGTTGTTGCTATTACTGTTGTTGTTGTTTCTGTCTCTGTTTTTGAATTGTTCTTCAGATTCTTGTTGCCGTGGAGGACTTATAGTTAACTCAAGATTCAAATCCGGACACCGCTCTAACACCGACCCTTTAATCATCATCTCTTGATGATGATTATGATAATGATTATGATATTGTTCTTGTTTAATAGACGGAGCGAAAGAGATAATTGATGTGGGTGTGGTAGCTGCTATAACAGTAGAAGTAGTAGTTGATACAGATGCAGCAGCAGCTACAGCTATAGTTACACCTTCTTGATTCTGGTTCTGGTTCTGGTTCTGAAGATGAAGAACTTGAGAATGAGTTTGAGATTGAGATTGAGATTGAGAATGAGAATGAGAAACCTCGTTTAAAGGTCTATGAGTAGCAGGGTCAATTCCTCTATTCAAAAGCTTTCTTCTAATATGAGTATTCCAATAATTCTTTATCTCATTATCTGTTCTACCGGGTAATCTTCCAGCTATCAAAGACCATTTGTTACCAAGAAGACTATGAAGTTTGATAATAAGTTCATCTTCTTCTTCGGTAAAGTTCCCGCGTTTAAGGTCCGGTCGGAGATAATTAATCCAGCGGAGACGGCAGCTTTTGCCGCAGCGGAGTAAACCAGCAGCTTTAGGAAGAGATCTCCAACAACCTTCACCGTGAGCACGAATATAAGAAATCAATCTATCGTCTTCTTCTTTAGTCCAAGCACCTTTGTTAGTATGAGCTTTTTCACAACAGGGTGATCTTCCCATGATTTGTTATGGAAAAACTAGAAAACAAGAAGAAAACAGAAAGCCCTCTGCGTTGAATACCACTGCTT
TARGET=478,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1301
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=CAGCTTTCAAACCCAAGAAATC
PRIMER_RIGHT_SEQUENCE=AACTATAAGTCCTCCACGGCAA
PRIMER_LEFT=336,22
PRIMER_RIGHT=547,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.1438
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=GTAATCCCAAACACCAGCTTTC
PRIMER_RIGHT_1_SEQUENCE=AACTATAAGTCCTCCACGGCAA
PRIMER_LEFT_1=322,22
PRIMER_RIGHT_1=547,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.2183
PRIMER_LEFT_2_PENALTY=0.197140
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=CCAACAATCTCGTCACAGCTAC
PRIMER_RIGHT_2_SEQUENCE=AACTATAAGTCCTCCACGGCAA
PRIMER_LEFT_2=389,22
PRIMER_RIGHT_2=547,22
PRIMER_LEFT_2_TM=59.803
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.3102
PRIMER_LEFT_3_PENALTY=0.108929
PRIMER_RIGHT_3_PENALTY=0.201288
PRIMER_LEFT_3_SEQUENCE=CAGCTTTCAAACCCAAGAAATC
PRIMER_RIGHT_3_SEQUENCE=GAGATGATGATTAAAGGGTCGG
PRIMER_LEFT_3=336,22
PRIMER_RIGHT_3=603,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.799
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.3239
PRIMER_LEFT_4_PENALTY=0.122569
PRIMER_RIGHT_4_PENALTY=0.201288
PRIMER_LEFT_4_SEQUENCE=GTAATCCCAAACACCAGCTTTC
PRIMER_RIGHT_4_SEQUENCE=GAGATGATGATTAAAGGGTCGG
PRIMER_LEFT_4=322,22
PRIMER_RIGHT_4=603,22
PRIMER_LEFT_4_TM=59.877
PRIMER_RIGHT_4_TM=59.799
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_03454 Ps_contig_mira-and-tgicl-ass_franssen_30036
SEQUENCE=TCAACGCAGAGTACGCGGGGATTCCTCAAGATTTGAGATAGAAAGAGAAAGCGGCTGCTTAGAAAGTTAGAGAGAGAGAGAAATAGAGTTGAAGACGGTGTGAGAGAGAGTCCTAGTTGAAGTAAAAAAAGATCATCAAACACAAGCAAACAACAGTTTCTTCTTCTTCCACCGATCCGTACTATGCCTTCTCGCAGAAGAACTCTCTTAAAGGTCATCATTCTCGGTGACAGCGGTGTGGGGAAGACGTCTTTGATGAACCAATATGTGAATAAGAAGTTTAGTAATCAGTACAAGGCAACCATTGGAGCGGATTTCTTAACCAAAGAAGTGCAATTTGAAGATAGGCTTTTCACCTTACAGATTTGGGATACAGCTGGCCAGGAGAGATTCCAAAGCCTAGGAGTTGCTTTCTATCGTGGTGCTGATTGCTGTGTTCTTGTATATGATGTTAATTCAGTGAAGTCATTTGACAACCTTAATAACTGGAGGGAAGAGTTTCTCATTCAAGCAAATCCTTCTGATCCAGAGAATTTTCCCTTTGTCGTTATAGGAAACAAGATAGATATTGATGGTGGAAACAGTAGAGTGGTTTCTGAAAAGAAGGCTCGGGCATGGTGTGCAGCAAAAGGAAATATCCCATATTTTGAGACATCTGCTAAAGAAGGTATTAATGTTGAAGAAGCATTCCAAACCATAGCAAAGGATGCCCTGAAAAGTGGGGAAGAGGAAGAATTATACCTGCCGGACACAATTGATGTTGGAAACAGCAGTCAGCCAAGGTCAACAGGATGTGAGTGCTGAACATATAGATTTTGTTCTCAATACAAAATAAAGTATATTATTTAAAAATCATTTTGGCATGTCTAGCCATTGCTGTCTATGAGGTTTTATTGTACATTTATGTTTGATCAAGTGCGATCTGTTGGGTGCTTGTTTGGCTTGTGTTAATCGATCATGTTGTTCTCCTTGTATGCTATTCCAACATTGTGAAAAAAACAGCAAAGGATCATTCTGAAGTTATTTCATTAGTTATTTTTCTTTCCCGTACAAAATATTGTAATCAATGGTGATTGGTGGTTGTGGTGATGCTTTGAGTTATAAAATTTGTTTAGAAAAACG
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2593
PRIMER_LEFT_PENALTY=0.115769
PRIMER_RIGHT_PENALTY=0.143555
PRIMER_LEFT_SEQUENCE=TGAGATAGAAAGAGAAAGCGGC
PRIMER_RIGHT_SEQUENCE=ATAGTACGGATCGGTGGAAGAA
PRIMER_LEFT=33,22
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=59.856
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=12.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=0.3269
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.211105
PRIMER_LEFT_1_SEQUENCE=TGAGATAGAAAGAGAAAGCGGC
PRIMER_RIGHT_1_SEQUENCE=CGGTGGAAGAAGAAGAAACTGT
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=173,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=59.789
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=12.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.3788
PRIMER_LEFT_2_PENALTY=0.115769
PRIMER_RIGHT_2_PENALTY=0.263023
PRIMER_LEFT_2_SEQUENCE=TGAGATAGAAAGAGAAAGCGGC
PRIMER_RIGHT_2_SEQUENCE=TCTTTGGTTAAGAAATCCGCTC
PRIMER_LEFT_2=33,22
PRIMER_RIGHT_2=328,22
PRIMER_LEFT_2_TM=60.116
PRIMER_RIGHT_2_TM=59.737
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=12.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=0.4238
PRIMER_LEFT_3_PENALTY=0.280218
PRIMER_RIGHT_3_PENALTY=0.143555
PRIMER_LEFT_3_SEQUENCE=AAAGCGGCTGCTTAGAAAGTTA
PRIMER_RIGHT_3_SEQUENCE=ATAGTACGGATCGGTGGAAGAA
PRIMER_LEFT_3=47,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=59.720
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=0.4854
PRIMER_LEFT_4_PENALTY=0.115769
PRIMER_RIGHT_4_PENALTY=0.369628
PRIMER_LEFT_4_SEQUENCE=TGAGATAGAAAGAGAAAGCGGC
PRIMER_RIGHT_4_SEQUENCE=GTCACCGAGAATGATGACCTTT
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=230,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=60.370
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=12.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=198
=
PRIMER_SEQUENCE_ID=SSR_03455 Ps_contig_mira-and-tgicl-ass_franssen_30036
SEQUENCE=TCAACGCAGAGTACGCGGGGATTCCTCAAGATTTGAGATAGAAAGAGAAAGCGGCTGCTTAGAAAGTTAGAGAGAGAGAGAAATAGAGTTGAAGACGGTGTGAGAGAGAGTCCTAGTTGAAGTAAAAAAAGATCATCAAACACAAGCAAACAACAGTTTCTTCTTCTTCCACCGATCCGTACTATGCCTTCTCGCAGAAGAACTCTCTTAAAGGTCATCATTCTCGGTGACAGCGGTGTGGGGAAGACGTCTTTGATGAACCAATATGTGAATAAGAAGTTTAGTAATCAGTACAAGGCAACCATTGGAGCGGATTTCTTAACCAAAGAAGTGCAATTTGAAGATAGGCTTTTCACCTTACAGATTTGGGATACAGCTGGCCAGGAGAGATTCCAAAGCCTAGGAGTTGCTTTCTATCGTGGTGCTGATTGCTGTGTTCTTGTATATGATGTTAATTCAGTGAAGTCATTTGACAACCTTAATAACTGGAGGGAAGAGTTTCTCATTCAAGCAAATCCTTCTGATCCAGAGAATTTTCCCTTTGTCGTTATAGGAAACAAGATAGATATTGATGGTGGAAACAGTAGAGTGGTTTCTGAAAAGAAGGCTCGGGCATGGTGTGCAGCAAAAGGAAATATCCCATATTTTGAGACATCTGCTAAAGAAGGTATTAATGTTGAAGAAGCATTCCAAACCATAGCAAAGGATGCCCTGAAAAGTGGGGAAGAGGAAGAATTATACCTGCCGGACACAATTGATGTTGGAAACAGCAGTCAGCCAAGGTCAACAGGATGTGAGTGCTGAACATATAGATTTTGTTCTCAATACAAAATAAAGTATATTATTTAAAAATCATTTTGGCATGTCTAGCCATTGCTGTCTATGAGGTTTTATTGTACATTTATGTTTGATCAAGTGCGATCTGTTGGGTGCTTGTTTGGCTTGTGTTAATCGATCATGTTGTTCTCCTTGTATGCTATTCCAACATTGTGAAAAAAACAGCAAAGGATCATTCTGAAGTTATTTCATTAGTTATTTTTCTTTCCCGTACAAAATATTGTAATCAATGGTGATTGGTGGTTGTGGTGATGCTTTGAGTTATAAAATTTGTTTAGAAAAACG
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3464
PRIMER_LEFT_PENALTY=0.083403
PRIMER_RIGHT_PENALTY=0.263023
PRIMER_LEFT_SEQUENCE=AAGACGGTGTGAGAGAGAGTCC
PRIMER_RIGHT_SEQUENCE=TCTTTGGTTAAGAAATCCGCTC
PRIMER_LEFT=91,22
PRIMER_RIGHT=328,22
PRIMER_LEFT_TM=59.917
PRIMER_RIGHT_TM=59.737
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.3788
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.263023
PRIMER_LEFT_1_SEQUENCE=TGAGATAGAAAGAGAAAGCGGC
PRIMER_RIGHT_1_SEQUENCE=TCTTTGGTTAAGAAATCCGCTC
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=328,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=59.737
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=12.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=0.4293
PRIMER_LEFT_2_PENALTY=0.166289
PRIMER_RIGHT_2_PENALTY=0.263023
PRIMER_LEFT_2_SEQUENCE=GCGGCTGCTTAGAAAGTTAGAG
PRIMER_RIGHT_2_SEQUENCE=TCTTTGGTTAAGAAATCCGCTC
PRIMER_LEFT_2=50,22
PRIMER_RIGHT_2=328,22
PRIMER_LEFT_2_TM=59.834
PRIMER_RIGHT_2_TM=59.737
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.4293
PRIMER_LEFT_3_PENALTY=0.166289
PRIMER_RIGHT_3_PENALTY=0.263023
PRIMER_LEFT_3_SEQUENCE=AGCGGCTGCTTAGAAAGTTAGA
PRIMER_RIGHT_3_SEQUENCE=TCTTTGGTTAAGAAATCCGCTC
PRIMER_LEFT_3=49,22
PRIMER_RIGHT_3=328,22
PRIMER_LEFT_3_TM=59.834
PRIMER_RIGHT_3_TM=59.737
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.4530
PRIMER_LEFT_4_PENALTY=0.083403
PRIMER_RIGHT_4_PENALTY=0.369628
PRIMER_LEFT_4_SEQUENCE=AAGACGGTGTGAGAGAGAGTCC
PRIMER_RIGHT_4_SEQUENCE=GTCACCGAGAATGATGACCTTT
PRIMER_LEFT_4=91,22
PRIMER_RIGHT_4=230,22
PRIMER_LEFT_4_TM=59.917
PRIMER_RIGHT_4_TM=60.370
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=140
=
PRIMER_SEQUENCE_ID=SSR_03456 Ps_contig_mira-and-tgicl-ass_franssen_30041
SEQUENCE=CCGCCAACAACAACAATAGACTCAGATACTCTTTCTTTCTTTATCTCCATCAGTCTTCGAATCAATTCATTTTACTCAACTCTCGTGGGAACAAAATTCTCCAACATTCCTCCTACGTTTCTTTTTAATCAAAAAACAGTTGTTAAGGTTCTCTGCTACCTCTTCTCCATTTTCATTAACTCATACAAAAAAAAACATCATCATTTTTTGTCGTAACAGATCAATATGGCTCTATCACGTTTATCTTCTTCTTCTTCTTCTTCCATCGGATCCAATCTCTTCAAACCCTTCTCCGCCGCCTTCACTCTCAACCGTCCGATCTCCTCCGACACAACCGCCACTCTCACTATCGAAACCTCCCTCCCCTTCACCGCCCACAACTGCGAACCACCTTCCCGCTCCGTCACCACATCACCCTCCGAGCTTCTCTCGTTCTTCCGCACCATGGCCTTAATGCGTCGGATGGAAATCGCCGCCGACTCACTCTATAAAGCCAAACTCATCCGCGGGTTTTGCCACCTCTACGACGGTCAGGAAGCCGTCGCTGTCGGTATGGAAGCTGGAACGACGAAAAAGGACTGTATTATTACTGCTTACCGTGATCACTGTACTTTTCTCGGTCGCGGTGGGACTCTACTTGAGATTTATGCAGAGCTTATGGGAAGAAGGGATGGGTGTTCGAAAGGCAAAGGTGGTTCGATGCATTTTTATAAGAAGGATTCTGGGTTTTATGGGGGGCATGGGATTGTGGGTGCTCAAGTTCCTTTGGGGTGTGGTTTGGCTTTTGGGCAGAAGTATTTGAAAGTGANAGTGYNANNNNTGCNNNGTATNGAGANNRTAAAGAACCAGTGGCAGAGTATCTCCCCGGCTCAGGCTAAACGATTCACGAAATTCAGGGAAAGGAAAGGGCTTATTGAGAAAGATGTGGTGAGAACTGATAGATCGATCACTTTCTATGAGGGGGATTCTCATTTCATCATCACTCAATTCTTACCGAAAACATCTCATCATCA
TARGET=5,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03457 Ps_contig_mira-and-tgicl-ass_franssen_30041
SEQUENCE=CCGCCAACAACAACAATAGACTCAGATACTCTTTCTTTCTTTATCTCCATCAGTCTTCGAATCAATTCATTTTACTCAACTCTCGTGGGAACAAAATTCTCCAACATTCCTCCTACGTTTCTTTTTAATCAAAAAACAGTTGTTAAGGTTCTCTGCTACCTCTTCTCCATTTTCATTAACTCATACAAAAAAAAACATCATCATTTTTTGTCGTAACAGATCAATATGGCTCTATCACGTTTATCTTCTTCTTCTTCTTCTTCCATCGGATCCAATCTCTTCAAACCCTTCTCCGCCGCCTTCACTCTCAACCGTCCGATCTCCTCCGACACAACCGCCACTCTCACTATCGAAACCTCCCTCCCCTTCACCGCCCACAACTGCGAACCACCTTCCCGCTCCGTCACCACATCACCCTCCGAGCTTCTCTCGTTCTTCCGCACCATGGCCTTAATGCGTCGGATGGAAATCGCCGCCGACTCACTCTATAAAGCCAAACTCATCCGCGGGTTTTGCCACCTCTACGACGGTCAGGAAGCCGTCGCTGTCGGTATGGAAGCTGGAACGACGAAAAAGGACTGTATTATTACTGCTTACCGTGATCACTGTACTTTTCTCGGTCGCGGTGGGACTCTACTTGAGATTTATGCAGAGCTTATGGGAAGAAGGGATGGGTGTTCGAAAGGCAAAGGTGGTTCGATGCATTTTTATAAGAAGGATTCTGGGTTTTATGGGGGGCATGGGATTGTGGGTGCTCAAGTTCCTTTGGGGTGTGGTTTGGCTTTTGGGCAGAAGTATTTGAAAGTGANAGTGYNANNNNTGCNNNGTATNGAGANNRTAAAGAACCAGTGGCAGAGTATCTCCCCGGCTCAGGCTAAACGATTCACGAAATTCAGGGAAAGGAAAGGGCTTATTGAGAAAGATGTGGTGAGAACTGATAGATCGATCACTTTCTATGAGGGGGATTCTCATTTCATCATCACTCAATTCTTACCGAAAACATCTCATCATCA
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03458 Ps_contig_mira-and-tgicl-ass_franssen_30041
SEQUENCE=CCGCCAACAACAACAATAGACTCAGATACTCTTTCTTTCTTTATCTCCATCAGTCTTCGAATCAATTCATTTTACTCAACTCTCGTGGGAACAAAATTCTCCAACATTCCTCCTACGTTTCTTTTTAATCAAAAAACAGTTGTTAAGGTTCTCTGCTACCTCTTCTCCATTTTCATTAACTCATACAAAAAAAAACATCATCATTTTTTGTCGTAACAGATCAATATGGCTCTATCACGTTTATCTTCTTCTTCTTCTTCTTCCATCGGATCCAATCTCTTCAAACCCTTCTCCGCCGCCTTCACTCTCAACCGTCCGATCTCCTCCGACACAACCGCCACTCTCACTATCGAAACCTCCCTCCCCTTCACCGCCCACAACTGCGAACCACCTTCCCGCTCCGTCACCACATCACCCTCCGAGCTTCTCTCGTTCTTCCGCACCATGGCCTTAATGCGTCGGATGGAAATCGCCGCCGACTCACTCTATAAAGCCAAACTCATCCGCGGGTTTTGCCACCTCTACGACGGTCAGGAAGCCGTCGCTGTCGGTATGGAAGCTGGAACGACGAAAAAGGACTGTATTATTACTGCTTACCGTGATCACTGTACTTTTCTCGGTCGCGGTGGGACTCTACTTGAGATTTATGCAGAGCTTATGGGAAGAAGGGATGGGTGTTCGAAAGGCAAAGGTGGTTCGATGCATTTTTATAAGAAGGATTCTGGGTTTTATGGGGGGCATGGGATTGTGGGTGCTCAAGTTCCTTTGGGGTGTGGTTTGGCTTTTGGGCAGAAGTATTTGAAAGTGANAGTGYNANNNNTGCNNNGTATNGAGANNRTAAAGAACCAGTGGCAGAGTATCTCCCCGGCTCAGGCTAAACGATTCACGAAATTCAGGGAAAGGAAAGGGCTTATTGAGAAAGATGTGGTGAGAACTGATAGATCGATCACTTTCTATGAGGGGGATTCTCATTTCATCATCACTCAATTCTTACCGAAAACATCTCATCATCA
TARGET=244,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03459 Ps_contig_mira-and-tgicl-ass_franssen_30042
SEQUENCE=ACTGACAAGAAGCGGCAATTGGAACCGATTCCGGTTAGTTATTCTTATTCCTTCGCATCTTTTTTCATTCTCATTTCATCATCACTCAATTCTTACCGAAAACAAATCTCATCATCACTCAATTCTTACCGAAAACAATCTCATCATCACTCACTACCACTTCTTCTTCTTCTTCTTCCATTCTAACATTGCTTCCACTTCACCACCACCATTCATATTCTTAAAGGATCAAATTAGGGATTGAATATCATGGATCGGCTTATATCTTCTGCACGGCTCATGATTGTTTCGGATCTCGATCACACAATGGTTGATCATCATGACACGGAGAATAGTTCCCTTTTGAGGTTCAACGCCCTATGGGAATCCACTTATCGCCACGATTCTCTTCTCGTGTTTTCAACTGGAAGGTCTCCTACACTCTACAAACAGCTGAGGAAAGAG
TARGET=160,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4300
PRIMER_LEFT_PENALTY=0.424445
PRIMER_RIGHT_PENALTY=0.005520
PRIMER_LEFT_SEQUENCE=GGAACCGATTCCGGTTAGTTAT
PRIMER_RIGHT_SEQUENCE=CGATCCATGATATTCAATCCCT
PRIMER_LEFT=20,22
PRIMER_RIGHT=256,22
PRIMER_LEFT_TM=60.424
PRIMER_RIGHT_TM=60.006
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.5684
PRIMER_LEFT_1_PENALTY=0.566087
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=CCGAAAACAATCTCATCATCAC
PRIMER_RIGHT_1_SEQUENCE=AAGGGAACTATTCTCCGTGTCA
PRIMER_LEFT_1=128,22
PRIMER_RIGHT_1=341,22
PRIMER_LEFT_1_TM=59.434
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=0.5684
PRIMER_LEFT_2_PENALTY=0.424445
PRIMER_RIGHT_2_PENALTY=0.143968
PRIMER_LEFT_2_SEQUENCE=GGAACCGATTCCGGTTAGTTAT
PRIMER_RIGHT_2_SEQUENCE=AGCCGATCCATGATATTCAATC
PRIMER_LEFT_2=20,22
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=60.424
PRIMER_RIGHT_2_TM=60.144
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=0.5716
PRIMER_LEFT_3_PENALTY=0.566087
PRIMER_RIGHT_3_PENALTY=0.005520
PRIMER_LEFT_3_SEQUENCE=CCGAAAACAATCTCATCATCAC
PRIMER_RIGHT_3_SEQUENCE=CGATCCATGATATTCAATCCCT
PRIMER_LEFT_3=128,22
PRIMER_RIGHT_3=256,22
PRIMER_LEFT_3_TM=59.434
PRIMER_RIGHT_3_TM=60.006
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.5718
PRIMER_LEFT_4_PENALTY=0.566087
PRIMER_RIGHT_4_PENALTY=0.005674
PRIMER_LEFT_4_SEQUENCE=CCGAAAACAATCTCATCATCAC
PRIMER_RIGHT_4_SEQUENCE=CAAAAGGGAACTATTCTCCGTG
PRIMER_LEFT_4=128,22
PRIMER_RIGHT_4=344,22
PRIMER_LEFT_4_TM=59.434
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_03460 Ps_contig_mira-and-tgicl-ass_franssen_30062
SEQUENCE=TTACTCNCTTTTCTNCCCAATCACACACAAAAGNGAACCAAATCGAAGATNCGGAAAGCGAAGCCAAGAAGCACACGGCGAAGGAGATCCAAGCCAAGGTGGACGCCGCGCTGACCAACCGCGGCGGNGGTAAAAGTTCCGGTTTTTGTTTTCTTGATCGGTATCGGTGTTGAAGTAAGGGAGGACACGCCAAGTGGGAGTGCCCTCACTGCAAAACAACGGTACCTGATGTGAAATCGAATGCAGATTCATCACGATGCTCGTCACCCGAAGATCCCCTTTGAGGAAGATAAGCTCGTGAATCGTCACGCATCTGCAGCTGCTGTTCCGGAATCTTCCAAGCCTCGCCCTGGTGTTCGTGGTAGCCTCAAAAAGTGATCGTTATCATCATCATCATCAAATCGCAGCCTTTCATTCATGGCTTTTACTATATTTTCTATTTTGTACTTTTTTTCTTCAAGTCGTCGTTTTTAGGGTGTTATTGAATAATTCGTGTCATGGGTCTGCTTAGTTATTTTGCCTTTTTTTTAAATATCAAGATGATGAATTTTAGGTATGAATGTTGTGTTTGAACTGTTGCGATGAAAGGTTTTTGATGGGAATCGGATTGAGTTGTGCTGTTATTATTAATTTGGCCCTTCTTAAGCCTTGAATATGTAACATATTGTTCCCTTGATGGCAGTTAATATGATCGTGCTACTACTTAACTTAAANNAAGNNTTATGATATATTGTTCNGTATAAAA
TARGET=384,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0974
PRIMER_LEFT_PENALTY=0.062402
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=ATCCCCTTTGAGGAAGATAAGC
PRIMER_RIGHT_SEQUENCE=ACCCTAAAAACGACGACTTGAA
PRIMER_LEFT=273,22
PRIMER_RIGHT=476,22
PRIMER_LEFT_TM=59.938
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=0.1232
PRIMER_LEFT_1_PENALTY=0.088198
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=GAAGATCCCCTTTGAGGAAGAT
PRIMER_RIGHT_1_SEQUENCE=ACCCTAAAAACGACGACTTGAA
PRIMER_LEFT_1=269,22
PRIMER_RIGHT_1=476,22
PRIMER_LEFT_1_TM=59.912
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=0.1576
PRIMER_LEFT_2_PENALTY=0.122569
PRIMER_RIGHT_2_PENALTY=0.035021
PRIMER_LEFT_2_SEQUENCE=AGGAAGATAAGCTCGTGAATCG
PRIMER_RIGHT_2_SEQUENCE=ACCCTAAAAACGACGACTTGAA
PRIMER_LEFT_2=283,22
PRIMER_RIGHT_2=476,22
PRIMER_LEFT_2_TM=59.877
PRIMER_RIGHT_2_TM=60.035
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.2038
PRIMER_LEFT_3_PENALTY=0.122569
PRIMER_RIGHT_3_PENALTY=0.081185
PRIMER_LEFT_3_SEQUENCE=AGGAAGATAAGCTCGTGAATCG
PRIMER_RIGHT_3_SEQUENCE=ATCGCAACAGTTCAAACACAAC
PRIMER_LEFT_3=283,22
PRIMER_RIGHT_3=582,22
PRIMER_LEFT_3_TM=59.877
PRIMER_RIGHT_3_TM=60.081
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.2827
PRIMER_LEFT_4_PENALTY=0.247716
PRIMER_RIGHT_4_PENALTY=0.035021
PRIMER_LEFT_4_SEQUENCE=GGAAGATAAGCTCGTGAATCGT
PRIMER_RIGHT_4_SEQUENCE=ACCCTAAAAACGACGACTTGAA
PRIMER_LEFT_4=284,22
PRIMER_RIGHT_4=476,22
PRIMER_LEFT_4_TM=59.752
PRIMER_RIGHT_4_TM=60.035
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_03461 Ps_contig_mira-and-tgicl-ass_franssen_30063
SEQUENCE=TTTNNTTTTTTATTTTTAAAATGTAATGCACAATTTTATATATCAAACAATCTTAAAACCACATTGTATACATTACATTTTACTCATACTTAGCTACCATAACAGATAAAAACCTACTAATACAAACATGTAACATACTATACTACAACAACAACAACAACATAAATACCTCTTTTTTAAAACCAAAACACAGACTAAGTAAGATCCCATACAAACAAGTAAGCCCTAAGGGCACTTGGTTTTGTTCCCATGGGTAGTCATGTCAGTGTAACAAGCTCCACAAAGCTCCCTGTTACCAGAAGTACCAGGTGGAACACACTTGCACCTCACACAACAAGTTCCACATGCTCTGTTACACAAGTTTGGCCTTGAATGTACACTGCATCTCGAACTGCACAATCCTCCACAATCTATGTCTTGCATTAGCCTTCTGTTGTCGTCTCGCACRGTGAGCANCNNACTTCTAAGTTTAGGGTAAGCTTAGGTAAACGTT
TARGET=145,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03462 Ps_contig_mira-and-tgicl-ass_franssen_30064
SEQUENCE=AACACATNTATANATTACATTTTACTTCATACTTAGTCTACCATAACANATAAAACTACTAATACAAACAATGTAACATACTATACTACAACAACAACAACAACATAAATACCTCTTTTTAAAACCTAAAACACAGACTAAGTAAGATCCCATACAAACAAGTAAGCCCTAAGGGCACTTGGTTTTGTTCCCATGGGTAGTCATGTCAGTGTAACAAGCTCCACAAAGCTCCCTGTTACCAGAAGTACCAGGTGGAACACACTTGCACCTCACACAACAAGTTCCACATGCTCTGTTACACAAGTTTGGCCTTGAATGTACACTGCATCTCGAACTGCACAATCCTCCACAATCTATGTCTTGCATTAGCCTTCTGTTGCCGTCTCGCACAGTGAGCAACTCATTAGGTAGCTGAAGTTCTTCTTCATCTTCTTCTTCCATTTCTATCTCATGCTCAGATGAAACCTGCATCAGATTAGAGAGGCATACTAGAAGCAATAAACCCAGCACAAAAAGGTTACGCAATGCCATTGCAGAATATTGTAGCAGTTTAAGTTGTGATGAGTATTATAGTTTGTGGATCCCCGAACTCTGCGTTGATTACCTTACTTTTGTTTCTT
TARGET=88,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03463 Ps_contig_mira-and-tgicl-ass_franssen_30066
SEQUENCE=TCGGGGACACTCACATTATTACATGATCCAACCAAAGAAGCTTAATAGCTTCTCTTGAGTTTCAATTGTATCTTATTCAGTGATTGATTTTAGGAATTTGTGTAATGCAGATTTTCACTCGAAGCCAAGGAGCACTTGTGTGCTTTTGGCTGTCTCTTGCTTTTTTGGTAATAGGATCTTGCCATAGTACAGTTGAGGTAGTTGGCCTTGGAGAATGCGCAGACTGCAAGCAGAACAACATTAAAACTAGTCAGGTTTTCTCAGGGCTAAAAGTAACAATAGACTGCAAGGAAGAAAGTGGACATTTTAAAACAAGAGGGGCTGGTGAGCTTGATAACAATGGAAATTTCAAAGTATCACTTCCAGAGGACATTGTTAAAGAAGGTGAACTGAAAGAAGAGTGTTATGCTCAGCTGCATAGTGCATCGGCTATACCATGTCCTGCTCATGATGGTCTGCAAGACACCAAAATTGTGATTAGTTCAAAATCTGATGAGAAACATACTCTCAGTACTGCTGCTGCTGGTAAACTCAAGTTTTCATCAGCAACATGTGCTTCAGCATTTTTTTTGGCCTTTTTATAAACATCCACTTTTTCCTAAACTTCCTCTTCCTCATTTCCCACCTAAAGTTTTTCCACCATTTCCACCAAAGTTTTTCCATAAGCATCACCCTCTCTTTCCTCCTATTCCTATCTACAAGAAACCTCTCCCTCCACCTGTGCCTATCTATGAGAACCCTCTCCCTCCCCCTACGCCGGTATATGAGAAACCTCTCCCTCCTCCTGTATATGAGAAACCTCTCCCTCCACCGGTCTACGAGAAACCTCTCCCTCCACCTGTCTATGAGAAACCTCTTCCTCCTCCTGTGCCCGTTTATCATAAACCCCCTACGCCGGTATATGAGAAACCTCTCCCTCCGCCTGTCTATGAGAAACCTCTTCCTCCTCCTGTGCCCGTTTATCATAAACCGCCTACGCCTGTTTATCATGAACCGCCAACACCTGTTTATCATAAACCTCCTACGCCCGTTTATCATAAACCGCCTACACCGGTTTATCATGAACCACCTACGCCCGTTTATCATAAACCGCCTACGCCTGTTTATCATAAACCTCCAACGCCGGTTTATCATGAACCTCCTACGCCTGTCTATCATGAACCGCCTACGCCTGTCTATCATGAACCGCCTACGCCTGTCTATCATAAACCGCCTACACCAGTCTATCATAAACCGCCTACACCAGTCTATCATGAACCGCCTACGCCTGTTTATCATAAACCGCCTACACCAGTTTATCATGAACCGCCTACGCCTGTTTATCATAAACCACATCCACCACAGGTTCCAGTCAAGAAACCTTGTCCACCAAAGGTTGAACATCCAATTCTACCACCTGTTCCAATCTACAAACCTCATCCTTCTCCAGTTCCAAAGCCACTTCCACCGCCTGTTCCGGTCAAAAAACCGTGTCCACCAAAGGTTGAGCATCCCATTCTACCACCTGTTCCAATATACAAACCTCCAGTAGTAGTTCCAAAGCCACCTGTGGTTCCAATATACAAACCTCCAGTAGTAATTCCAAAGCCACCTGTGGTTCCAATATACAAACCTCCAGTAGTAATTCCAAAACCACCTGTGGTTCCAATATACAAACCTCCAGTAGTAGTTCCAATCATCAAGCCCCATCCTCCTCTTGTTCCAATCTATAAACCACCAGTTGTTATCAAACCTCTTCCTCCTTTCCCAAAGTTTCCTCCATTCAACAAACCACCATTTCCACCTCTTCCCAAGTTCCCTCCAGTGCATGATTTTTTCCATCACCCCAAGTTCGGAAAATTGCCTCCATTGCCTCCAAAAAGCTTCTTCCATCACCCCAAGTATGGCAAATGGCCACCAATCCATAAGAGCTTCTTCCATCACCCCAAATATGGAAAATGGCCAACGGATCCAAAAAGCTTCTTCCATCACCCCAAGCTTGGGAAATGGCCACCATTAACTCCTCATCACTAAGAGCCATGTTTGAGCTTATATAATACTTTCACTGCTTTTCGTCTTCTTCGCTTGTTTAAATACTATCATCGATCAATTTGGAAATAAAGCCTTCGAGGGACATAGAGTGTGTGGGCAGAAAAACAGACATAGTACGATGTTGTTTGAAGAAGTTTGATGAAGCTGAGTGTAATCACTTGTTCCGGAATTATTTTTTCTTAAAATTACTTTCATGTATATATAATTGTGTTATTTGTGATGGTTTTCTCACTTTCTGTCTTTGTATTACGAATTCTTACTTTATGTTCAATTAATAAATCCATTTGTATAACGTTAATCTAANTNACTAAAGNTTNAAAAAGTT
TARGET=514,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1859
PRIMER_LEFT_PENALTY=0.002623
PRIMER_RIGHT_PENALTY=0.183296
PRIMER_LEFT_SEQUENCE=CATTTTAAAACAAGAGGGGCTG
PRIMER_RIGHT_SEQUENCE=GCACATGTTGCTGATGAAAACT
PRIMER_LEFT=302,22
PRIMER_RIGHT=555,22
PRIMER_LEFT_TM=59.997
PRIMER_RIGHT_TM=60.183
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.4024
PRIMER_LEFT_1_PENALTY=0.368304
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=GGCTATACCATGTCCTGCTCAT
PRIMER_RIGHT_1_SEQUENCE=AGAGGGTGATGCTTATGGAAAA
PRIMER_LEFT_1=427,22
PRIMER_RIGHT_1=676,22
PRIMER_LEFT_1_TM=60.368
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.4639
PRIMER_LEFT_2_PENALTY=0.368304
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=GGCTATACCATGTCCTGCTCAT
PRIMER_RIGHT_2_SEQUENCE=AGAGAGGGTGATGCTTATGGAA
PRIMER_LEFT_2=427,22
PRIMER_RIGHT_2=678,22
PRIMER_LEFT_2_TM=60.368
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=0.4639
PRIMER_LEFT_3_PENALTY=0.368304
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=GGCTATACCATGTCCTGCTCAT
PRIMER_RIGHT_3_SEQUENCE=AAGAGAGGGTGATGCTTATGGA
PRIMER_LEFT_3=427,22
PRIMER_RIGHT_3=679,22
PRIMER_LEFT_3_TM=60.368
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=253
PRIMER_PAIR_PENALTY_4=0.4722
PRIMER_LEFT_4_PENALTY=0.288917
PRIMER_RIGHT_4_PENALTY=0.183296
PRIMER_LEFT_4_SEQUENCE=GACTGCAAGGAAGAAAGTGGAC
PRIMER_RIGHT_4_SEQUENCE=GCACATGTTGCTGATGAAAACT
PRIMER_LEFT_4=281,22
PRIMER_RIGHT_4=555,22
PRIMER_LEFT_4_TM=60.289
PRIMER_RIGHT_4_TM=60.183
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_03464 Ps_contig_mira-and-tgicl-ass_franssen_30072
SEQUENCE=GGGACATCACTACACAAACAACACTCCCATCTTTAACTCTGGCTCCTACACTTCACTTCAACTCAACACTCAAAGCATCTCTCTCTTTCTTCCTCACTGCCATCACCCATTTTCCCAAAATGGGTAATCACTCTCTCTTCCTCCTCTTCCCTCTCCTCCTCCTCTCCATCACCACCACCATCTTCGCCCACAACATCACCGAAATCCTCTCCGACAACCCTGACTACAGCCAATACAACAACTTCCTTTCCCAAACCAAACTCGCCGACGAAATCAACTCCCGTCAAACCATCACAGTCTTAGTCCTCAACAACGCTGCATTCTCTTCCATCACCTCAAAACACCCCCTCTCCGTCGTTAAAAAAGTCCTCAGCCTCCTCATCCTCCTCGACTACTTCGACAACTCCAAACTCCACCAAATCACCAACGGCACCACTCTCTCCACCACTCTCTTCCAAACCACCGGCAACGCTCAGGGAAACATCGGATCCGTCAACATCACCGATTTAAAAGGAGGTAAAGTAGGCTTCGGTTCCGCCACACCTGGATCCAAACTAGATTCATCTTACTCCAAATCCATCAAACAAATCCCTTACAATATCTCCGTTCTCGAAATCTCCGCTCCGATCATCGCTCCCGGAATCCTAACCGCTCCTCCTCCGTCTAGCGACGTTAACATAACCGCTTTAATAGAAAAAGCTGGATGCAAAACGTTTGCGTCGTTAATCCTATCTAACGGTCTTATCAAAACCTTCCAATCAACTGCTGATAAAGGTTTAACCATTTTTGCTCCGAACGATGAAGCTTTCAAAGCTGACGGAGTTCCAGATTTAACCAAACTCACCAACGCTGAAACGGTTTCGCTCTTACAGTATCATGCAGCTGCTAAATATCTTCCCATTGGATCTTTGAAAACTACTAAGGATCCTATTAGTAGTTTAGCTGCTAACGGCGCCGGAAAGTTTGATTACTCAGTTTCCGTTGCCGGTGATTCTGTTACACTTCACACCGGTGTTGATTCCTCTAGGATCGCTGATACTATTTTGGATTCTACTCCACTTTCTATTTACTCTGTTGACAGTGTGCTTCTTCCTCCTGAGTTGTTTGCTAAGTCGCCATCTCCTGCTCCGGCACCGGAGCCTGCCGGTGCTCCTTCTCCAACACCTGCTTCTGCTCCCTCGCCGGTGGCTGATGCGCCTACTCCATTACCTGCCTCGCCTCCGGCTCCTGCTGGTGAGAGTCCAAGCGATTCTCCGGCTGATGCACCGTCGACTGCGGCGGAGCGTAGTAGCTCCGGGAAATCTGCTGGTGTTCACGTGAAAGCTTCTGCTGCTCTCACTTTTACAGTGACTACTTTGTGTGCTTTTGTGATTTCTTCTGTTTTCATGTCCTGATTTGTAATTTTAGAGTGTTGTTGTTTTATTATTTTGTAATTTAAATTTAAATTTTTGAGTTTTTTTTTCTTCTTCTCTGAATGTGATTGGATTAGAGATCTTTATTTCGTTATAAATTAATTTAGGTATGGACCGTTGTACGTGAATGATGCTTTGCTTTGATTTTAATTTTATAGTACTCTATTTTTAATATTAAGTTTTTTTANTTTAAANCAGTGCTAATTGTTTATTTTAATTTTGAAAGTAAAA
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1166
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=TGGCTCCTACACTTCACTTCAA
PRIMER_RIGHT_SEQUENCE=TAAGACTGTGATGGTTTGACGG
PRIMER_LEFT=39,22
PRIMER_RIGHT=301,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.1606
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=CACTCCCATCTTTAACTCTGGC
PRIMER_RIGHT_1_SEQUENCE=TAAGACTGTGATGGTTTGACGG
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=301,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.1620
PRIMER_LEFT_2_PENALTY=0.131699
PRIMER_RIGHT_2_PENALTY=0.030276
PRIMER_LEFT_2_SEQUENCE=TCTCTCTTTCTTCCTCACTGCC
PRIMER_RIGHT_2_SEQUENCE=TAAGACTGTGATGGTTTGACGG
PRIMER_LEFT_2=79,22
PRIMER_RIGHT_2=301,22
PRIMER_LEFT_2_TM=60.132
PRIMER_RIGHT_2_TM=60.030
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.3265
PRIMER_LEFT_3_PENALTY=0.296177
PRIMER_RIGHT_3_PENALTY=0.030276
PRIMER_LEFT_3_SEQUENCE=CCCAAAATGGGTAATCACTCTC
PRIMER_RIGHT_3_SEQUENCE=TAAGACTGTGATGGTTTGACGG
PRIMER_LEFT_3=113,22
PRIMER_RIGHT_3=301,22
PRIMER_LEFT_3_TM=59.704
PRIMER_RIGHT_3_TM=60.030
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.3265
PRIMER_LEFT_4_PENALTY=0.296177
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=TCCCAAAATGGGTAATCACTCT
PRIMER_RIGHT_4_SEQUENCE=TAAGACTGTGATGGTTTGACGG
PRIMER_LEFT_4=112,22
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=59.704
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_03465 Ps_contig_mira-and-tgicl-ass_franssen_30083
SEQUENCE=AAATTTNGATGAACTGCCAAGCTAAGCCAAGTAAGGTTGACACTTTTTTACACCAACTACTCTCTCTCTCTCTCAAGCTTATCCCTCTCCACCACTTGTTCTATCTTCTTATTCTTCTTCTTCTTCTTCTTCTTTTGTTCTAGCCACCAAAACAATAATCATCCAACAAGATGAACAACGGTGGTGAACAATACTCTGCTATTGAGACACAGTACATAAGAAGACATCACAAACATGATTTAAGAGACAATCAGTGTTCCTCTGCACTTGTTAAACACATCAAAGCCCCTGTTCATCTTGTTTGGTCTCTGGTTAGAAGATTTGATCAGCCACAGAAATATAAACCATTTGTTAGCAGGTGTATTATGCAAGGTGATCTTGGCATTGGAAGTGTTAGAGAAGTGAATGTTAAATCTGGTCTTCCAGCTACAACTAGTACTGAGAGGTTGGAACAGCTTGATGATGAAGAACATATTCTCGGTATCAGGATTGTTGGTGGCGATCATAGGCTCAGGAACTATTCCTCCGTAATCACTGTCCATCCGGAGGTTATTGATGGAAGACCAGGTACAATGGTGATTGAATCGTTCGTCGTGGACGTGCCTGAAGGGAACACGAGAGATGAAACGTGTTACTTCGTCGAGGCGTTGATCAGGTGCAACCTGAGTTCTTTAGCGGATGTCTCGGAGAGGATGGCCGTGCAGGGTCGAACCGATCCTATCAATGTTAATCCGTAAGAATGTATAAAGGGTTATGATCCTTTGATTTCTGATCCCAAGTCCAAGTTTGGAGTATGGTTGGAAACATGAATTTTATTCCTCACAAGTTTTGGATGTTTTGTTTTTCTTCTCTTATCATGGACACATTACATTCTCCCCAAACCATATCCCTACAACTTTTTTAGATGTGTGTTCTTGTTTCGAAAACCGACGTTAGGATCCTCAGCTCTGCTCGCGGAGAGGTTATTGACTATGGTTTTTGTATGAGGATCTAGATTTCATAAACACAAGTGAGTTTGGGAATGGAAAAGTTGTTTTTTTACTTGTTAAATTCTTCTTGGATTTTGTGGATGGTGTTCTGTTATTACAGTTATTTGTAAATACTTTTGTTGGAAAAAAAAGACTATTTATGCTTTTTGTTTAGTAGCAAATTAGAAAGAAGTAGGTTTGTAAGTTATGTATTTTACATTCTACTTGTTTAGGACTTTGTTTAGAACAAAATAAAACTTGTTTTGANACTAGTTACTTTTTC
TARGET=60,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2403
PRIMER_LEFT_PENALTY=0.193532
PRIMER_RIGHT_PENALTY=0.046801
PRIMER_LEFT_SEQUENCE=TTNGATGAACTGCCAAGCTAAG
PRIMER_RIGHT_SEQUENCE=AACAAGTGCAGAGGAACACTGA
PRIMER_LEFT=4,22
PRIMER_RIGHT=271,22
PRIMER_LEFT_TM=59.806
PRIMER_RIGHT_TM=59.953
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=0.4185
PRIMER_LEFT_1_PENALTY=0.193532
PRIMER_RIGHT_1_PENALTY=0.224933
PRIMER_LEFT_1_SEQUENCE=TTNGATGAACTGCCAAGCTAAG
PRIMER_RIGHT_1_SEQUENCE=GTGCAGAGGAACACTGATTGTC
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=266,22
PRIMER_LEFT_1_TM=59.806
PRIMER_RIGHT_1_TM=59.775
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.4797
PRIMER_LEFT_2_PENALTY=0.432914
PRIMER_RIGHT_2_PENALTY=0.046801
PRIMER_LEFT_2_SEQUENCE=ACTGCCAAGCTAAGCCAAGTAA
PRIMER_RIGHT_2_SEQUENCE=AACAAGTGCAGAGGAACACTGA
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=271,22
PRIMER_LEFT_2_TM=60.433
PRIMER_RIGHT_2_TM=59.953
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.4797
PRIMER_LEFT_3_PENALTY=0.432914
PRIMER_RIGHT_3_PENALTY=0.046801
PRIMER_LEFT_3_SEQUENCE=AACTGCCAAGCTAAGCCAAGTA
PRIMER_RIGHT_3_SEQUENCE=AACAAGTGCAGAGGAACACTGA
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=271,22
PRIMER_LEFT_3_TM=60.433
PRIMER_RIGHT_3_TM=59.953
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.5956
PRIMER_LEFT_4_PENALTY=0.548772
PRIMER_RIGHT_4_PENALTY=0.046801
PRIMER_LEFT_4_SEQUENCE=CTGCCAAGCTAAGCCAAGTAAG
PRIMER_RIGHT_4_SEQUENCE=AACAAGTGCAGAGGAACACTGA
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=271,22
PRIMER_LEFT_4_TM=60.549
PRIMER_RIGHT_4_TM=59.953
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=259
=
PRIMER_SEQUENCE_ID=SSR_03466 Ps_contig_mira-and-tgicl-ass_franssen_30083
SEQUENCE=AAATTTNGATGAACTGCCAAGCTAAGCCAAGTAAGGTTGACACTTTTTTACACCAACTACTCTCTCTCTCTCTCAAGCTTATCCCTCTCCACCACTTGTTCTATCTTCTTATTCTTCTTCTTCTTCTTCTTCTTTTGTTCTAGCCACCAAAACAATAATCATCCAACAAGATGAACAACGGTGGTGAACAATACTCTGCTATTGAGACACAGTACATAAGAAGACATCACAAACATGATTTAAGAGACAATCAGTGTTCCTCTGCACTTGTTAAACACATCAAAGCCCCTGTTCATCTTGTTTGGTCTCTGGTTAGAAGATTTGATCAGCCACAGAAATATAAACCATTTGTTAGCAGGTGTATTATGCAAGGTGATCTTGGCATTGGAAGTGTTAGAGAAGTGAATGTTAAATCTGGTCTTCCAGCTACAACTAGTACTGAGAGGTTGGAACAGCTTGATGATGAAGAACATATTCTCGGTATCAGGATTGTTGGTGGCGATCATAGGCTCAGGAACTATTCCTCCGTAATCACTGTCCATCCGGAGGTTATTGATGGAAGACCAGGTACAATGGTGATTGAATCGTTCGTCGTGGACGTGCCTGAAGGGAACACGAGAGATGAAACGTGTTACTTCGTCGAGGCGTTGATCAGGTGCAACCTGAGTTCTTTAGCGGATGTCTCGGAGAGGATGGCCGTGCAGGGTCGAACCGATCCTATCAATGTTAATCCGTAAGAATGTATAAAGGGTTATGATCCTTTGATTTCTGATCCCAAGTCCAAGTTTGGAGTATGGTTGGAAACATGAATTTTATTCCTCACAAGTTTTGGATGTTTTGTTTTTCTTCTCTTATCATGGACACATTACATTCTCCCCAAACCATATCCCTACAACTTTTTTAGATGTGTGTTCTTGTTTCGAAAACCGACGTTAGGATCCTCAGCTCTGCTCGCGGAGAGGTTATTGACTATGGTTTTTGTATGAGGATCTAGATTTCATAAACACAAGTGAGTTTGGGAATGGAAAAGTTGTTTTTTTACTTGTTAAATTCTTCTTGGATTTTGTGGATGGTGTTCTGTTATTACAGTTATTTGTAAATACTTTTGTTGGAAAAAAAAGACTATTTATGCTTTTTGTTTAGTAGCAAATTAGAAAGAAGTAGGTTTGTAAGTTATGTATTTTACATTCTACTTGTTTAGGACTTTGTTTAGAACAAAATAAAACTTGTTTTGANACTAGTTACTTTTTC
TARGET=112,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1607
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.024665
PRIMER_LEFT_SEQUENCE=TTATCCCTCTCCACCACTTGTT
PRIMER_RIGHT_SEQUENCE=TCACCTTGCATAATACACCTGC
PRIMER_LEFT=78,22
PRIMER_RIGHT=375,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.025
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=0.2403
PRIMER_LEFT_1_PENALTY=0.193532
PRIMER_RIGHT_1_PENALTY=0.046801
PRIMER_LEFT_1_SEQUENCE=TTNGATGAACTGCCAAGCTAAG
PRIMER_RIGHT_1_SEQUENCE=AACAAGTGCAGAGGAACACTGA
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=271,22
PRIMER_LEFT_1_TM=59.806
PRIMER_RIGHT_1_TM=59.953
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.3609
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.224933
PRIMER_LEFT_2_SEQUENCE=TTATCCCTCTCCACCACTTGTT
PRIMER_RIGHT_2_SEQUENCE=GTGCAGAGGAACACTGATTGTC
PRIMER_LEFT_2=78,22
PRIMER_RIGHT_2=266,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.775
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.3861
PRIMER_LEFT_3_PENALTY=0.361394
PRIMER_RIGHT_3_PENALTY=0.024665
PRIMER_LEFT_3_SEQUENCE=TATCCCTCTCCACCACTTGTTC
PRIMER_RIGHT_3_SEQUENCE=TCACCTTGCATAATACACCTGC
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=375,22
PRIMER_LEFT_3_TM=60.361
PRIMER_RIGHT_3_TM=60.025
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.4082
PRIMER_LEFT_4_PENALTY=0.361394
PRIMER_RIGHT_4_PENALTY=0.046801
PRIMER_LEFT_4_SEQUENCE=TATCCCTCTCCACCACTTGTTC
PRIMER_RIGHT_4_SEQUENCE=AACAAGTGCAGAGGAACACTGA
PRIMER_LEFT_4=79,22
PRIMER_RIGHT_4=271,22
PRIMER_LEFT_4_TM=60.361
PRIMER_RIGHT_4_TM=59.953
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_03467 Ps_contig_mira-and-tgicl-ass_franssen_30096
SEQUENCE=AAGTTTCTTAAAAAATTTAAAAAACTTAAAACAAACTTTACCTTTACTTACTTTACTACCTTAACGTTTAACTTTAACTTAAGGTTAAGTTAATTTTACTTACTTAAATTTAAAACCTTTTTAAACCCCCTTTACCCCTACCCGGTCCGGTTAACTTTACTAACTACACTCAACCCTTTACCCTTACTTACTTCCGTACCCCCGAACCCCCAACCCCAACCTATCCNACTCCACCCTTCCTACCTACCAACCTCTCTCTCTCATCATCATCATCATCATCATCATGCAGTTTCTTCATCTATCCATTTTCATCTATACACCGTTGCGCATAATGTCTTATTTCTTTTTCTATTCGCACCTCTCAAATCAAACTCCCGATTCCCATTTCTTCCTCCCTGTTAATTCCACTCTTCCGAACAATACCATTGAAGCGTCGGCGGTTGTTCGTCGGAAAAGAAATTACAAGTCTCATCGGCACTAAGAAAATTATGCTCTTCCATTTTCACATACTGTGTCTGATACTCATTCTGAAACTGAAAACAATTACTCATCGAAGACGGCGAAGAAGATGAAGAACCACCAAACTTCAACGAAGAGGATCCATTAGACATCAAAAAGCTTTGATTTTGAAGAACCCCAGATGAAGAAATCGCTGCTTTGGGACTGTTATTGTTTTCTTCGTTCAAGATTGCGCTTGAGTCGCTATCCGAAGAACCGTCTTTGAAATCCGCAGGTAACAGAGAAGCTACTACATCGGTGTTTCTGAAACAGATGTCCTTAGAATCGGATCTAAGAATGGCCGTTTCATCGGAAGCTGTGTTTTCTGTGTCTTGCAATGTTATCAACTCTTGTTTTACACAAACATCACTTTCTATAGTTGTGTTTTCTTCTTCCATTTGTAATCTTGATTTCAATTCCTTAACCTCTTTGAGTAAGGTTTCATTATCACGTTGAAGCGTCTCATAATTAACCTTAAGTGCATCAAAGTTAGCTTTAAGAACTCCATAATCTCTTTCCAACTGTTTTGTTTTCCATCTAGCACGACGGTTTTGAAACCAAACCGCAACTTGTCTTGGTTGCAAACCAAGTTCTTGAGCAAGCTTCACTTTCCTATCAGGTTCAAGCTTGTTTTCAACTTCAAAATTCTTCTCCAAAGCCTTCACTTGATCCACACTCAGTCTTCGTTTCTTCTGCCCTACTTCTTCAACACACCCTTCTTCATCGAACCCTTCCAACATCGACTGAAATTCCCTACCATACACGTTGTTGTTGTTGCTGCTACTGCTGTGGTTGTTCCTCGGACTATGTTCCTCTGTTTGTGGACAGATTGTGATGAGAGCACCTAAAGAATCGGAGCTGGAAAGTCTCTTCATGAGTACACGTAATATTAGTAAAAGATTAAGGTTGTTAAATATGATTAGTGAAAATTGAGATCTGAAGAGAAGAGAAAAGAAAGGTTTTGTTTTGTTTTGTTTTACTTAAATGGAGAGTTTAGGGTATCTTAAACAACGCCAATTAGCACAATTGTTTAAGCAATTACTAAACAAAAACAACCACTTATCACAACCATTAATAACTTCCATGTGTCTATCTTTGTTTTTTCTCACCTCTTCACCCATCAAACAACTCCAACACAACACAACAAATTGAACTAGAGACCAATAAGTATGTTTTTTTTTTCTAGACACCACTGTTTT
TARGET=252,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1127
PRIMER_LEFT_PENALTY=0.051552
PRIMER_RIGHT_PENALTY=0.061184
PRIMER_LEFT_SEQUENCE=TTTTAAACCCCCTTTACCCCTA
PRIMER_RIGHT_SEQUENCE=TGGAATTAACAGGGAGGAAGAA
PRIMER_LEFT=118,22
PRIMER_RIGHT=406,22
PRIMER_LEFT_TM=59.948
PRIMER_RIGHT_TM=59.939
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.1412
PRIMER_LEFT_1_PENALTY=0.120932
PRIMER_RIGHT_1_PENALTY=0.020269
PRIMER_LEFT_1_SEQUENCE=CCTTACTTACTTCCGTACCCCC
PRIMER_RIGHT_1_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT_1=181,22
PRIMER_RIGHT_1=326,22
PRIMER_LEFT_1_TM=60.121
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=12.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.1428
PRIMER_LEFT_2_PENALTY=0.122569
PRIMER_RIGHT_2_PENALTY=0.020269
PRIMER_LEFT_2_SEQUENCE=NACTCCACCCTTCCTACCTACC
PRIMER_RIGHT_2_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT_2=226,22
PRIMER_RIGHT_2=326,22
PRIMER_LEFT_2_TM=59.877
PRIMER_RIGHT_2_TM=60.020
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=101
PRIMER_PAIR_PENALTY_3=0.1821
PRIMER_LEFT_3_PENALTY=0.120932
PRIMER_RIGHT_3_PENALTY=0.061184
PRIMER_LEFT_3_SEQUENCE=CCTTACTTACTTCCGTACCCCC
PRIMER_RIGHT_3_SEQUENCE=TGGAATTAACAGGGAGGAAGAA
PRIMER_LEFT_3=181,22
PRIMER_RIGHT_3=406,22
PRIMER_LEFT_3_TM=60.121
PRIMER_RIGHT_3_TM=59.939
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=12.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.2064
PRIMER_LEFT_4_PENALTY=0.051552
PRIMER_RIGHT_4_PENALTY=0.154878
PRIMER_LEFT_4_SEQUENCE=TTTTAAACCCCCTTTACCCCTA
PRIMER_RIGHT_4_SEQUENCE=TGATTTGAGAGGTGCGAATAGA
PRIMER_LEFT_4=118,22
PRIMER_RIGHT_4=368,22
PRIMER_LEFT_4_TM=59.948
PRIMER_RIGHT_4_TM=59.845
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_03468 Ps_contig_mira-and-tgicl-ass_franssen_30096
SEQUENCE=AAGTTTCTTAAAAAATTTAAAAAACTTAAAACAAACTTTACCTTTACTTACTTTACTACCTTAACGTTTAACTTTAACTTAAGGTTAAGTTAATTTTACTTACTTAAATTTAAAACCTTTTTAAACCCCCTTTACCCCTACCCGGTCCGGTTAACTTTACTAACTACACTCAACCCTTTACCCTTACTTACTTCCGTACCCCCGAACCCCCAACCCCAACCTATCCNACTCCACCCTTCCTACCTACCAACCTCTCTCTCTCATCATCATCATCATCATCATCATGCAGTTTCTTCATCTATCCATTTTCATCTATACACCGTTGCGCATAATGTCTTATTTCTTTTTCTATTCGCACCTCTCAAATCAAACTCCCGATTCCCATTTCTTCCTCCCTGTTAATTCCACTCTTCCGAACAATACCATTGAAGCGTCGGCGGTTGTTCGTCGGAAAAGAAATTACAAGTCTCATCGGCACTAAGAAAATTATGCTCTTCCATTTTCACATACTGTGTCTGATACTCATTCTGAAACTGAAAACAATTACTCATCGAAGACGGCGAAGAAGATGAAGAACCACCAAACTTCAACGAAGAGGATCCATTAGACATCAAAAAGCTTTGATTTTGAAGAACCCCAGATGAAGAAATCGCTGCTTTGGGACTGTTATTGTTTTCTTCGTTCAAGATTGCGCTTGAGTCGCTATCCGAAGAACCGTCTTTGAAATCCGCAGGTAACAGAGAAGCTACTACATCGGTGTTTCTGAAACAGATGTCCTTAGAATCGGATCTAAGAATGGCCGTTTCATCGGAAGCTGTGTTTTCTGTGTCTTGCAATGTTATCAACTCTTGTTTTACACAAACATCACTTTCTATAGTTGTGTTTTCTTCTTCCATTTGTAATCTTGATTTCAATTCCTTAACCTCTTTGAGTAAGGTTTCATTATCACGTTGAAGCGTCTCATAATTAACCTTAAGTGCATCAAAGTTAGCTTTAAGAACTCCATAATCTCTTTCCAACTGTTTTGTTTTCCATCTAGCACGACGGTTTTGAAACCAAACCGCAACTTGTCTTGGTTGCAAACCAAGTTCTTGAGCAAGCTTCACTTTCCTATCAGGTTCAAGCTTGTTTTCAACTTCAAAATTCTTCTCCAAAGCCTTCACTTGATCCACACTCAGTCTTCGTTTCTTCTGCCCTACTTCTTCAACACACCCTTCTTCATCGAACCCTTCCAACATCGACTGAAATTCCCTACCATACACGTTGTTGTTGTTGCTGCTACTGCTGTGGTTGTTCCTCGGACTATGTTCCTCTGTTTGTGGACAGATTGTGATGAGAGCACCTAAAGAATCGGAGCTGGAAAGTCTCTTCATGAGTACACGTAATATTAGTAAAAGATTAAGGTTGTTAAATATGATTAGTGAAAATTGAGATCTGAAGAGAAGAGAAAAGAAAGGTTTTGTTTTGTTTTGTTTTACTTAAATGGAGAGTTTAGGGTATCTTAAACAACGCCAATTAGCACAATTGTTTAAGCAATTACTAAACAAAAACAACCACTTATCACAACCATTAATAACTTCCATGTGTCTATCTTTGTTTTTTCTCACCTCTTCACCCATCAAACAACTCCAACACAACACAACAAATTGAACTAGAGACCAATAAGTATGTTTTTTTTTTCTAGACACCACTGTTTT
TARGET=261,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1127
PRIMER_LEFT_PENALTY=0.051552
PRIMER_RIGHT_PENALTY=0.061184
PRIMER_LEFT_SEQUENCE=TTTTAAACCCCCTTTACCCCTA
PRIMER_RIGHT_SEQUENCE=TGGAATTAACAGGGAGGAAGAA
PRIMER_LEFT=118,22
PRIMER_RIGHT=406,22
PRIMER_LEFT_TM=59.948
PRIMER_RIGHT_TM=59.939
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.1412
PRIMER_LEFT_1_PENALTY=0.120932
PRIMER_RIGHT_1_PENALTY=0.020269
PRIMER_LEFT_1_SEQUENCE=CCTTACTTACTTCCGTACCCCC
PRIMER_RIGHT_1_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT_1=181,22
PRIMER_RIGHT_1=326,22
PRIMER_LEFT_1_TM=60.121
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=12.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.1428
PRIMER_LEFT_2_PENALTY=0.122569
PRIMER_RIGHT_2_PENALTY=0.020269
PRIMER_LEFT_2_SEQUENCE=NACTCCACCCTTCCTACCTACC
PRIMER_RIGHT_2_SEQUENCE=CGCAACGGTGTATAGATGAAAA
PRIMER_LEFT_2=226,22
PRIMER_RIGHT_2=326,22
PRIMER_LEFT_2_TM=59.877
PRIMER_RIGHT_2_TM=60.020
PRIMER_LEFT_2_GC_PERCENT=57.143
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=101
PRIMER_PAIR_PENALTY_3=0.1724
PRIMER_LEFT_3_PENALTY=0.111172
PRIMER_RIGHT_3_PENALTY=0.061184
PRIMER_LEFT_3_SEQUENCE=CCCTTCCTACCTACCAACCTCT
PRIMER_RIGHT_3_SEQUENCE=TGGAATTAACAGGGAGGAAGAA
PRIMER_LEFT_3=233,22
PRIMER_RIGHT_3=406,22
PRIMER_LEFT_3_TM=59.889
PRIMER_RIGHT_3_TM=59.939
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.1821
PRIMER_LEFT_4_PENALTY=0.120932
PRIMER_RIGHT_4_PENALTY=0.061184
PRIMER_LEFT_4_SEQUENCE=CCTTACTTACTTCCGTACCCCC
PRIMER_RIGHT_4_SEQUENCE=TGGAATTAACAGGGAGGAAGAA
PRIMER_LEFT_4=181,22
PRIMER_RIGHT_4=406,22
PRIMER_LEFT_4_TM=60.121
PRIMER_RIGHT_4_TM=59.939
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=12.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_03469 Ps_contig_mira-and-tgicl-ass_franssen_30096
SEQUENCE=AAGTTTCTTAAAAAATTTAAAAAACTTAAAACAAACTTTACCTTTACTTACTTTACTACCTTAACGTTTAACTTTAACTTAAGGTTAAGTTAATTTTACTTACTTAAATTTAAAACCTTTTTAAACCCCCTTTACCCCTACCCGGTCCGGTTAACTTTACTAACTACACTCAACCCTTTACCCTTACTTACTTCCGTACCCCCGAACCCCCAACCCCAACCTATCCNACTCCACCCTTCCTACCTACCAACCTCTCTCTCTCATCATCATCATCATCATCATCATGCAGTTTCTTCATCTATCCATTTTCATCTATACACCGTTGCGCATAATGTCTTATTTCTTTTTCTATTCGCACCTCTCAAATCAAACTCCCGATTCCCATTTCTTCCTCCCTGTTAATTCCACTCTTCCGAACAATACCATTGAAGCGTCGGCGGTTGTTCGTCGGAAAAGAAATTACAAGTCTCATCGGCACTAAGAAAATTATGCTCTTCCATTTTCACATACTGTGTCTGATACTCATTCTGAAACTGAAAACAATTACTCATCGAAGACGGCGAAGAAGATGAAGAACCACCAAACTTCAACGAAGAGGATCCATTAGACATCAAAAAGCTTTGATTTTGAAGAACCCCAGATGAAGAAATCGCTGCTTTGGGACTGTTATTGTTTTCTTCGTTCAAGATTGCGCTTGAGTCGCTATCCGAAGAACCGTCTTTGAAATCCGCAGGTAACAGAGAAGCTACTACATCGGTGTTTCTGAAACAGATGTCCTTAGAATCGGATCTAAGAATGGCCGTTTCATCGGAAGCTGTGTTTTCTGTGTCTTGCAATGTTATCAACTCTTGTTTTACACAAACATCACTTTCTATAGTTGTGTTTTCTTCTTCCATTTGTAATCTTGATTTCAATTCCTTAACCTCTTTGAGTAAGGTTTCATTATCACGTTGAAGCGTCTCATAATTAACCTTAAGTGCATCAAAGTTAGCTTTAAGAACTCCATAATCTCTTTCCAACTGTTTTGTTTTCCATCTAGCACGACGGTTTTGAAACCAAACCGCAACTTGTCTTGGTTGCAAACCAAGTTCTTGAGCAAGCTTCACTTTCCTATCAGGTTCAAGCTTGTTTTCAACTTCAAAATTCTTCTCCAAAGCCTTCACTTGATCCACACTCAGTCTTCGTTTCTTCTGCCCTACTTCTTCAACACACCCTTCTTCATCGAACCCTTCCAACATCGACTGAAATTCCCTACCATACACGTTGTTGTTGTTGCTGCTACTGCTGTGGTTGTTCCTCGGACTATGTTCCTCTGTTTGTGGACAGATTGTGATGAGAGCACCTAAAGAATCGGAGCTGGAAAGTCTCTTCATGAGTACACGTAATATTAGTAAAAGATTAAGGTTGTTAAATATGATTAGTGAAAATTGAGATCTGAAGAGAAGAGAAAAGAAAGGTTTTGTTTTGTTTTGTTTTACTTAAATGGAGAGTTTAGGGTATCTTAAACAACGCCAATTAGCACAATTGTTTAAGCAATTACTAAACAAAAACAACCACTTATCACAACCATTAATAACTTCCATGTGTCTATCTTTGTTTTTTCTCACCTCTTCACCCATCAAACAACTCCAACACAACACAACAAATTGAACTAGAGACCAATAAGTATGTTTTTTTTTTCTAGACACCACTGTTTT
TARGET=1263,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0886
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=TCTGCCCTACTTCTTCAACACA
PRIMER_RIGHT_SEQUENCE=AGGAACATAGTCCGAGGAACAA
PRIMER_LEFT=1189,22
PRIMER_RIGHT=1311,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=0.0951
PRIMER_LEFT_1_PENALTY=0.092792
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=CTTGATCCACACTCAGTCTTCG
PRIMER_RIGHT_1_SEQUENCE=AGGAACATAGTCCGAGGAACAA
PRIMER_LEFT_1=1162,22
PRIMER_RIGHT_1=1311,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=150
PRIMER_PAIR_PENALTY_2=0.1101
PRIMER_LEFT_2_PENALTY=0.107763
PRIMER_RIGHT_2_PENALTY=0.002310
PRIMER_LEFT_2_SEQUENCE=TTCGTTTCTTCTGCCCTACTTC
PRIMER_RIGHT_2_SEQUENCE=AGGAACATAGTCCGAGGAACAA
PRIMER_LEFT_2=1180,22
PRIMER_RIGHT_2=1311,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=0.1768
PRIMER_LEFT_3_PENALTY=0.174518
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=AAGCCTTCACTTGATCCACACT
PRIMER_RIGHT_3_SEQUENCE=AGGAACATAGTCCGAGGAACAA
PRIMER_LEFT_3=1153,22
PRIMER_RIGHT_3=1311,22
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=0.1874
PRIMER_LEFT_4_PENALTY=0.185055
PRIMER_RIGHT_4_PENALTY=0.002310
PRIMER_LEFT_4_SEQUENCE=CTTGAGCAAGCTTCACTTTCCT
PRIMER_RIGHT_4_SEQUENCE=AGGAACATAGTCCGAGGAACAA
PRIMER_LEFT_4=1090,22
PRIMER_RIGHT_4=1311,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=59.998
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_03470 Ps_contig_mira-and-tgicl-ass_franssen_30096
SEQUENCE=AAGTTTCTTAAAAAATTTAAAAAACTTAAAACAAACTTTACCTTTACTTACTTTACTACCTTAACGTTTAACTTTAACTTAAGGTTAAGTTAATTTTACTTACTTAAATTTAAAACCTTTTTAAACCCCCTTTACCCCTACCCGGTCCGGTTAACTTTACTAACTACACTCAACCCTTTACCCTTACTTACTTCCGTACCCCCGAACCCCCAACCCCAACCTATCCNACTCCACCCTTCCTACCTACCAACCTCTCTCTCTCATCATCATCATCATCATCATCATGCAGTTTCTTCATCTATCCATTTTCATCTATACACCGTTGCGCATAATGTCTTATTTCTTTTTCTATTCGCACCTCTCAAATCAAACTCCCGATTCCCATTTCTTCCTCCCTGTTAATTCCACTCTTCCGAACAATACCATTGAAGCGTCGGCGGTTGTTCGTCGGAAAAGAAATTACAAGTCTCATCGGCACTAAGAAAATTATGCTCTTCCATTTTCACATACTGTGTCTGATACTCATTCTGAAACTGAAAACAATTACTCATCGAAGACGGCGAAGAAGATGAAGAACCACCAAACTTCAACGAAGAGGATCCATTAGACATCAAAAAGCTTTGATTTTGAAGAACCCCAGATGAAGAAATCGCTGCTTTGGGACTGTTATTGTTTTCTTCGTTCAAGATTGCGCTTGAGTCGCTATCCGAAGAACCGTCTTTGAAATCCGCAGGTAACAGAGAAGCTACTACATCGGTGTTTCTGAAACAGATGTCCTTAGAATCGGATCTAAGAATGGCCGTTTCATCGGAAGCTGTGTTTTCTGTGTCTTGCAATGTTATCAACTCTTGTTTTACACAAACATCACTTTCTATAGTTGTGTTTTCTTCTTCCATTTGTAATCTTGATTTCAATTCCTTAACCTCTTTGAGTAAGGTTTCATTATCACGTTGAAGCGTCTCATAATTAACCTTAAGTGCATCAAAGTTAGCTTTAAGAACTCCATAATCTCTTTCCAACTGTTTTGTTTTCCATCTAGCACGACGGTTTTGAAACCAAACCGCAACTTGTCTTGGTTGCAAACCAAGTTCTTGAGCAAGCTTCACTTTCCTATCAGGTTCAAGCTTGTTTTCAACTTCAAAATTCTTCTCCAAAGCCTTCACTTGATCCACACTCAGTCTTCGTTTCTTCTGCCCTACTTCTTCAACACACCCTTCTTCATCGAACCCTTCCAACATCGACTGAAATTCCCTACCATACACGTTGTTGTTGTTGCTGCTACTGCTGTGGTTGTTCCTCGGACTATGTTCCTCTGTTTGTGGACAGATTGTGATGAGAGCACCTAAAGAATCGGAGCTGGAAAGTCTCTTCATGAGTACACGTAATATTAGTAAAAGATTAAGGTTGTTAAATATGATTAGTGAAAATTGAGATCTGAAGAGAAGAGAAAAGAAAGGTTTTGTTTTGTTTTGTTTTACTTAAATGGAGAGTTTAGGGTATCTTAAACAACGCCAATTAGCACAATTGTTTAAGCAATTACTAAACAAAAACAACCACTTATCACAACCATTAATAACTTCCATGTGTCTATCTTTGTTTTTTCTCACCTCTTCACCCATCAAACAACTCCAACACAACACAACAAATTGAACTAGAGACCAATAAGTATGTTTTTTTTTTCTAGACACCACTGTTTT
TARGET=1457,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1038
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.101479
PRIMER_LEFT_SEQUENCE=TTGTTCCTCGGACTATGTTCCT
PRIMER_RIGHT_SEQUENCE=TTGGCGTTGTTTAAGATACCCT
PRIMER_LEFT=1290,22
PRIMER_RIGHT=1514,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.899
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.2313
PRIMER_LEFT_1_PENALTY=0.129818
PRIMER_RIGHT_1_PENALTY=0.101479
PRIMER_LEFT_1_SEQUENCE=GGTTGTTCCTCGGACTATGTTC
PRIMER_RIGHT_1_SEQUENCE=TTGGCGTTGTTTAAGATACCCT
PRIMER_LEFT_1=1288,22
PRIMER_RIGHT_1=1514,22
PRIMER_LEFT_1_TM=59.870
PRIMER_RIGHT_1_TM=59.899
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=227
PRIMER_PAIR_PENALTY_2=0.2332
PRIMER_LEFT_2_PENALTY=0.131699
PRIMER_RIGHT_2_PENALTY=0.101479
PRIMER_LEFT_2_SEQUENCE=TCCTCGGACTATGTTCCTCTGT
PRIMER_RIGHT_2_SEQUENCE=TTGGCGTTGTTTAAGATACCCT
PRIMER_LEFT_2=1294,22
PRIMER_RIGHT_2=1514,22
PRIMER_LEFT_2_TM=60.132
PRIMER_RIGHT_2_TM=59.899
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=221
PRIMER_PAIR_PENALTY_3=0.2500
PRIMER_LEFT_3_PENALTY=0.148509
PRIMER_RIGHT_3_PENALTY=0.101479
PRIMER_LEFT_3_SEQUENCE=TCCTCTGTTTGTGGACAGATTG
PRIMER_RIGHT_3_SEQUENCE=TTGGCGTTGTTTAAGATACCCT
PRIMER_LEFT_3=1308,22
PRIMER_RIGHT_3=1514,22
PRIMER_LEFT_3_TM=60.149
PRIMER_RIGHT_3_TM=59.899
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=0.2769
PRIMER_LEFT_4_PENALTY=0.253332
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=ATGAGAGCACCTAAAGAATCGG
PRIMER_RIGHT_4_SEQUENCE=ATGGGTGAAGAGGTGAGAAAAA
PRIMER_LEFT_4=1332,22
PRIMER_RIGHT_4=1618,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.8000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_03471 Ps_contig_mira-and-tgicl-ass_franssen_30096
SEQUENCE=AAGTTTCTTAAAAAATTTAAAAAACTTAAAACAAACTTTACCTTTACTTACTTTACTACCTTAACGTTTAACTTTAACTTAAGGTTAAGTTAATTTTACTTACTTAAATTTAAAACCTTTTTAAACCCCCTTTACCCCTACCCGGTCCGGTTAACTTTACTAACTACACTCAACCCTTTACCCTTACTTACTTCCGTACCCCCGAACCCCCAACCCCAACCTATCCNACTCCACCCTTCCTACCTACCAACCTCTCTCTCTCATCATCATCATCATCATCATCATGCAGTTTCTTCATCTATCCATTTTCATCTATACACCGTTGCGCATAATGTCTTATTTCTTTTTCTATTCGCACCTCTCAAATCAAACTCCCGATTCCCATTTCTTCCTCCCTGTTAATTCCACTCTTCCGAACAATACCATTGAAGCGTCGGCGGTTGTTCGTCGGAAAAGAAATTACAAGTCTCATCGGCACTAAGAAAATTATGCTCTTCCATTTTCACATACTGTGTCTGATACTCATTCTGAAACTGAAAACAATTACTCATCGAAGACGGCGAAGAAGATGAAGAACCACCAAACTTCAACGAAGAGGATCCATTAGACATCAAAAAGCTTTGATTTTGAAGAACCCCAGATGAAGAAATCGCTGCTTTGGGACTGTTATTGTTTTCTTCGTTCAAGATTGCGCTTGAGTCGCTATCCGAAGAACCGTCTTTGAAATCCGCAGGTAACAGAGAAGCTACTACATCGGTGTTTCTGAAACAGATGTCCTTAGAATCGGATCTAAGAATGGCCGTTTCATCGGAAGCTGTGTTTTCTGTGTCTTGCAATGTTATCAACTCTTGTTTTACACAAACATCACTTTCTATAGTTGTGTTTTCTTCTTCCATTTGTAATCTTGATTTCAATTCCTTAACCTCTTTGAGTAAGGTTTCATTATCACGTTGAAGCGTCTCATAATTAACCTTAAGTGCATCAAAGTTAGCTTTAAGAACTCCATAATCTCTTTCCAACTGTTTTGTTTTCCATCTAGCACGACGGTTTTGAAACCAAACCGCAACTTGTCTTGGTTGCAAACCAAGTTCTTGAGCAAGCTTCACTTTCCTATCAGGTTCAAGCTTGTTTTCAACTTCAAAATTCTTCTCCAAAGCCTTCACTTGATCCACACTCAGTCTTCGTTTCTTCTGCCCTACTTCTTCAACACACCCTTCTTCATCGAACCCTTCCAACATCGACTGAAATTCCCTACCATACACGTTGTTGTTGTTGCTGCTACTGCTGTGGTTGTTCCTCGGACTATGTTCCTCTGTTTGTGGACAGATTGTGATGAGAGCACCTAAAGAATCGGAGCTGGAAAGTCTCTTCATGAGTACACGTAATATTAGTAAAAGATTAAGGTTGTTAAATATGATTAGTGAAAATTGAGATCTGAAGAGAAGAGAAAAGAAAGGTTTTGTTTTGTTTTGTTTTACTTAAATGGAGAGTTTAGGGTATCTTAAACAACGCCAATTAGCACAATTGTTTAAGCAATTACTAAACAAAAACAACCACTTATCACAACCATTAATAACTTCCATGTGTCTATCTTTGTTTTTTCTCACCTCTTCACCCATCAAACAACTCCAACACAACACAACAAATTGAACTAGAGACCAATAAGTATGTTTTTTTTTTCTAGACACCACTGTTTT
TARGET=1630,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03472 Ps_contig_mira-and-tgicl-ass_franssen_30098
SEQUENCE=CTTACTTAGTCGTCCTCTCTCTCTCTCTCTATCCTATCCAGTTACTGAGTTAGTTATCATCGCAAAAAACTGATTGCAAGAAAGAGAAAATCCAAAATCCAAACTCATAGAACTAGTAATGTCTCTGAACGCTCTACTTCCACCTCCTCTATCGAGTTCCTCTTCAAGCTTACGCTCCTCCTCCTCCTTCAACCATTCTCTCTTTTCCATTCCAAAACAAATCCCCAAAAGAACTCACCACCACCACCACCACCATGTGTTGGTGGTTGAAGCAAAGGGTAAAAAAGGAATGATGTCCGGCCAATTCCAGCGCCCTCCCCCTCCTCCTCCTTTACCCAAAATCGAAGACGACGGTAACCCCAAATTCGTCGTCTTCATTCGCATGGCTAATATTTACCTTTGGTATCCTCTTAGTATCGTTTCCGGTGGCACCACCGCCAAAATCATGGTTGCCGCTAAAGATAACTTTCTCGGCAAATATATCTACAAGGATACTCTCGCTAGAAATCTCGCTTCTGTTATCTACAGAGATGAGAAGGAGATACAGAAAACTGCAATCAAACAACATCGAGTGCTGAAGTCAGCTACTGAATTCAGATATGGCTATAAACTTATTGTGAAGGGTAATGTAAGATCTGCACTTACTACCAATAATGTTATTGAGCTTCCTACACGAGATAAACTAAAAACGGTAGTTGATCAAGTGAAAGACTTTTTCGAAGATGTAAAAGACACCTTTTCGATGATAACATCGTTCGACGACGATACTACAACCAAGGCGCCAGAGAAAGATACAAAAGCAAAAACAAAATCTAAGGCTAAAAGACGATGATACTATAGAAGGAAACGATTTGCTTGAATTGTTATGTGCTACTTGGGGCAGCATGAGCTTTAGGAAGCAAGTTTCATGCTGTTTGTAGATTGGTCAATGGTTGCTTTCTTTGCACAACAAATGCTACTTGTGTTTTGTTCTTTATACCTGCACCCGACTAAATTATGCTCTAGAGTCGATATCACTTTTATGGTGGTGTTCACTCATTTATGTTTTGATTCATAATGCATTGGGATAGATAGCTTGTTCAAATTTCTTGATCTGCTGTTCAAATTAACAGTTGTAATATTCATGCATTTTATTTCCCAGGAATTCATTTTGTTGATCAGGAATATTACGTGTTATGGTCCTTTTTGCAATAATGTATGTATAAAACAAGAAAAAACAAAAACCT
TARGET=15,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03473 Ps_contig_mira-and-tgicl-ass_franssen_30098
SEQUENCE=CTTACTTAGTCGTCCTCTCTCTCTCTCTCTATCCTATCCAGTTACTGAGTTAGTTATCATCGCAAAAAACTGATTGCAAGAAAGAGAAAATCCAAAATCCAAACTCATAGAACTAGTAATGTCTCTGAACGCTCTACTTCCACCTCCTCTATCGAGTTCCTCTTCAAGCTTACGCTCCTCCTCCTCCTTCAACCATTCTCTCTTTTCCATTCCAAAACAAATCCCCAAAAGAACTCACCACCACCACCACCACCATGTGTTGGTGGTTGAAGCAAAGGGTAAAAAAGGAATGATGTCCGGCCAATTCCAGCGCCCTCCCCCTCCTCCTCCTTTACCCAAAATCGAAGACGACGGTAACCCCAAATTCGTCGTCTTCATTCGCATGGCTAATATTTACCTTTGGTATCCTCTTAGTATCGTTTCCGGTGGCACCACCGCCAAAATCATGGTTGCCGCTAAAGATAACTTTCTCGGCAAATATATCTACAAGGATACTCTCGCTAGAAATCTCGCTTCTGTTATCTACAGAGATGAGAAGGAGATACAGAAAACTGCAATCAAACAACATCGAGTGCTGAAGTCAGCTACTGAATTCAGATATGGCTATAAACTTATTGTGAAGGGTAATGTAAGATCTGCACTTACTACCAATAATGTTATTGAGCTTCCTACACGAGATAAACTAAAAACGGTAGTTGATCAAGTGAAAGACTTTTTCGAAGATGTAAAAGACACCTTTTCGATGATAACATCGTTCGACGACGATACTACAACCAAGGCGCCAGAGAAAGATACAAAAGCAAAAACAAAATCTAAGGCTAAAAGACGATGATACTATAGAAGGAAACGATTTGCTTGAATTGTTATGTGCTACTTGGGGCAGCATGAGCTTTAGGAAGCAAGTTTCATGCTGTTTGTAGATTGGTCAATGGTTGCTTTCTTTGCACAACAAATGCTACTTGTGTTTTGTTCTTTATACCTGCACCCGACTAAATTATGCTCTAGAGTCGATATCACTTTTATGGTGGTGTTCACTCATTTATGTTTTGATTCATAATGCATTGGGATAGATAGCTTGTTCAAATTTCTTGATCTGCTGTTCAAATTAACAGTTGTAATATTCATGCATTTTATTTCCCAGGAATTCATTTTGTTGATCAGGAATATTACGTGTTATGGTCCTTTTTGCAATAATGTATGTATAAAACAAGAAAAAACAAAAACCT
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0479
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.014720
PRIMER_LEFT_SEQUENCE=CGCTCTACTTCCACCTCCTCTA
PRIMER_RIGHT_SEQUENCE=CCACCGGAAACGATACTAAGAG
PRIMER_LEFT=129,22
PRIMER_RIGHT=428,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.3771
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.343977
PRIMER_LEFT_1_SEQUENCE=CGCTCTACTTCCACCTCCTCTA
PRIMER_RIGHT_1_SEQUENCE=GTCGTCTTCGATTTTGGGTAAA
PRIMER_LEFT_1=129,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.344
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.4084
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.375264
PRIMER_LEFT_2_SEQUENCE=CGCTCTACTTCCACCTCCTCTA
PRIMER_RIGHT_2_SEQUENCE=TTTTACCCTTTGCTTCAACCAC
PRIMER_LEFT_2=129,22
PRIMER_RIGHT_2=283,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=60.375
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.4953
PRIMER_LEFT_3_PENALTY=0.033137
PRIMER_RIGHT_3_PENALTY=0.462203
PRIMER_LEFT_3_SEQUENCE=CGCTCTACTTCCACCTCCTCTA
PRIMER_RIGHT_3_SEQUENCE=TCGTCTTCGATTTTGGGTAAAG
PRIMER_LEFT_3=129,22
PRIMER_RIGHT_3=350,22
PRIMER_LEFT_3_TM=60.033
PRIMER_RIGHT_3_TM=60.462
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.6312
PRIMER_LEFT_4_PENALTY=0.287182
PRIMER_RIGHT_4_PENALTY=0.343977
PRIMER_LEFT_4_SEQUENCE=TTCCACCTCCTCTATCGAGTTC
PRIMER_RIGHT_4_SEQUENCE=GTCGTCTTCGATTTTGGGTAAA
PRIMER_LEFT_4=137,22
PRIMER_RIGHT_4=351,22
PRIMER_LEFT_4_TM=59.713
PRIMER_RIGHT_4_TM=60.344
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_03474 Ps_contig_mira-and-tgicl-ass_franssen_30098
SEQUENCE=CTTACTTAGTCGTCCTCTCTCTCTCTCTCTATCCTATCCAGTTACTGAGTTAGTTATCATCGCAAAAAACTGATTGCAAGAAAGAGAAAATCCAAAATCCAAACTCATAGAACTAGTAATGTCTCTGAACGCTCTACTTCCACCTCCTCTATCGAGTTCCTCTTCAAGCTTACGCTCCTCCTCCTCCTTCAACCATTCTCTCTTTTCCATTCCAAAACAAATCCCCAAAAGAACTCACCACCACCACCACCACCATGTGTTGGTGGTTGAAGCAAAGGGTAAAAAAGGAATGATGTCCGGCCAATTCCAGCGCCCTCCCCCTCCTCCTCCTTTACCCAAAATCGAAGACGACGGTAACCCCAAATTCGTCGTCTTCATTCGCATGGCTAATATTTACCTTTGGTATCCTCTTAGTATCGTTTCCGGTGGCACCACCGCCAAAATCATGGTTGCCGCTAAAGATAACTTTCTCGGCAAATATATCTACAAGGATACTCTCGCTAGAAATCTCGCTTCTGTTATCTACAGAGATGAGAAGGAGATACAGAAAACTGCAATCAAACAACATCGAGTGCTGAAGTCAGCTACTGAATTCAGATATGGCTATAAACTTATTGTGAAGGGTAATGTAAGATCTGCACTTACTACCAATAATGTTATTGAGCTTCCTACACGAGATAAACTAAAAACGGTAGTTGATCAAGTGAAAGACTTTTTCGAAGATGTAAAAGACACCTTTTCGATGATAACATCGTTCGACGACGATACTACAACCAAGGCGCCAGAGAAAGATACAAAAGCAAAAACAAAATCTAAGGCTAAAAGACGATGATACTATAGAAGGAAACGATTTGCTTGAATTGTTATGTGCTACTTGGGGCAGCATGAGCTTTAGGAAGCAAGTTTCATGCTGTTTGTAGATTGGTCAATGGTTGCTTTCTTTGCACAACAAATGCTACTTGTGTTTTGTTCTTTATACCTGCACCCGACTAAATTATGCTCTAGAGTCGATATCACTTTTATGGTGGTGTTCACTCATTTATGTTTTGATTCATAATGCATTGGGATAGATAGCTTGTTCAAATTTCTTGATCTGCTGTTCAAATTAACAGTTGTAATATTCATGCATTTTATTTCCCAGGAATTCATTTTGTTGATCAGGAATATTACGTGTTATGGTCCTTTTTGCAATAATGTATGTATAAAACAAGAAAAAACAAAAACCT
TARGET=236,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0479
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.014720
PRIMER_LEFT_SEQUENCE=CGCTCTACTTCCACCTCCTCTA
PRIMER_RIGHT_SEQUENCE=CCACCGGAAACGATACTAAGAG
PRIMER_LEFT=129,22
PRIMER_RIGHT=428,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.3771
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.343977
PRIMER_LEFT_1_SEQUENCE=CGCTCTACTTCCACCTCCTCTA
PRIMER_RIGHT_1_SEQUENCE=GTCGTCTTCGATTTTGGGTAAA
PRIMER_LEFT_1=129,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.344
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.4084
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.375264
PRIMER_LEFT_2_SEQUENCE=CGCTCTACTTCCACCTCCTCTA
PRIMER_RIGHT_2_SEQUENCE=TTTTACCCTTTGCTTCAACCAC
PRIMER_LEFT_2=129,22
PRIMER_RIGHT_2=283,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=60.375
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.4376
PRIMER_LEFT_3_PENALTY=0.422888
PRIMER_RIGHT_3_PENALTY=0.014720
PRIMER_LEFT_3_SEQUENCE=CCATTCTCTCTTTTCCATTCCA
PRIMER_RIGHT_3_SEQUENCE=CACCGGAAACGATACTAAGAGG
PRIMER_LEFT_3=192,22
PRIMER_RIGHT_3=427,22
PRIMER_LEFT_3_TM=60.423
PRIMER_RIGHT_3_TM=60.015
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.4376
PRIMER_LEFT_4_PENALTY=0.422888
PRIMER_RIGHT_4_PENALTY=0.014720
PRIMER_LEFT_4_SEQUENCE=CCATTCTCTCTTTTCCATTCCA
PRIMER_RIGHT_4_SEQUENCE=CCACCGGAAACGATACTAAGAG
PRIMER_LEFT_4=192,22
PRIMER_RIGHT_4=428,22
PRIMER_LEFT_4_TM=60.423
PRIMER_RIGHT_4_TM=60.015
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_03475 Ps_contig_mira-and-tgicl-ass_franssen_30098
SEQUENCE=CTTACTTAGTCGTCCTCTCTCTCTCTCTCTATCCTATCCAGTTACTGAGTTAGTTATCATCGCAAAAAACTGATTGCAAGAAAGAGAAAATCCAAAATCCAAACTCATAGAACTAGTAATGTCTCTGAACGCTCTACTTCCACCTCCTCTATCGAGTTCCTCTTCAAGCTTACGCTCCTCCTCCTCCTTCAACCATTCTCTCTTTTCCATTCCAAAACAAATCCCCAAAAGAACTCACCACCACCACCACCACCATGTGTTGGTGGTTGAAGCAAAGGGTAAAAAAGGAATGATGTCCGGCCAATTCCAGCGCCCTCCCCCTCCTCCTCCTTTACCCAAAATCGAAGACGACGGTAACCCCAAATTCGTCGTCTTCATTCGCATGGCTAATATTTACCTTTGGTATCCTCTTAGTATCGTTTCCGGTGGCACCACCGCCAAAATCATGGTTGCCGCTAAAGATAACTTTCTCGGCAAATATATCTACAAGGATACTCTCGCTAGAAATCTCGCTTCTGTTATCTACAGAGATGAGAAGGAGATACAGAAAACTGCAATCAAACAACATCGAGTGCTGAAGTCAGCTACTGAATTCAGATATGGCTATAAACTTATTGTGAAGGGTAATGTAAGATCTGCACTTACTACCAATAATGTTATTGAGCTTCCTACACGAGATAAACTAAAAACGGTAGTTGATCAAGTGAAAGACTTTTTCGAAGATGTAAAAGACACCTTTTCGATGATAACATCGTTCGACGACGATACTACAACCAAGGCGCCAGAGAAAGATACAAAAGCAAAAACAAAATCTAAGGCTAAAAGACGATGATACTATAGAAGGAAACGATTTGCTTGAATTGTTATGTGCTACTTGGGGCAGCATGAGCTTTAGGAAGCAAGTTTCATGCTGTTTGTAGATTGGTCAATGGTTGCTTTCTTTGCACAACAAATGCTACTTGTGTTTTGTTCTTTATACCTGCACCCGACTAAATTATGCTCTAGAGTCGATATCACTTTTATGGTGGTGTTCACTCATTTATGTTTTGATTCATAATGCATTGGGATAGATAGCTTGTTCAAATTTCTTGATCTGCTGTTCAAATTAACAGTTGTAATATTCATGCATTTTATTTCCCAGGAATTCATTTTGTTGATCAGGAATATTACGTGTTATGGTCCTTTTTGCAATAATGTATGTATAAAACAAGAAAAAACAAAAACCT
TARGET=320,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0479
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.014720
PRIMER_LEFT_SEQUENCE=CGCTCTACTTCCACCTCCTCTA
PRIMER_RIGHT_SEQUENCE=CCACCGGAAACGATACTAAGAG
PRIMER_LEFT=129,22
PRIMER_RIGHT=428,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.3900
PRIMER_LEFT_1_PENALTY=0.375264
PRIMER_RIGHT_1_PENALTY=0.014720
PRIMER_LEFT_1_SEQUENCE=GTGGTTGAAGCAAAGGGTAAAA
PRIMER_RIGHT_1_SEQUENCE=CACCGGAAACGATACTAAGAGG
PRIMER_LEFT_1=262,22
PRIMER_RIGHT_1=427,22
PRIMER_LEFT_1_TM=60.375
PRIMER_RIGHT_1_TM=60.015
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=166
PRIMER_PAIR_PENALTY_2=0.3900
PRIMER_LEFT_2_PENALTY=0.375264
PRIMER_RIGHT_2_PENALTY=0.014720
PRIMER_LEFT_2_SEQUENCE=GTGGTTGAAGCAAAGGGTAAAA
PRIMER_RIGHT_2_SEQUENCE=CCACCGGAAACGATACTAAGAG
PRIMER_LEFT_2=262,22
PRIMER_RIGHT_2=428,22
PRIMER_LEFT_2_TM=60.375
PRIMER_RIGHT_2_TM=60.015
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.4376
PRIMER_LEFT_3_PENALTY=0.422888
PRIMER_RIGHT_3_PENALTY=0.014720
PRIMER_LEFT_3_SEQUENCE=CCATTCTCTCTTTTCCATTCCA
PRIMER_RIGHT_3_SEQUENCE=CACCGGAAACGATACTAAGAGG
PRIMER_LEFT_3=192,22
PRIMER_RIGHT_3=427,22
PRIMER_LEFT_3_TM=60.423
PRIMER_RIGHT_3_TM=60.015
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.4376
PRIMER_LEFT_4_PENALTY=0.422888
PRIMER_RIGHT_4_PENALTY=0.014720
PRIMER_LEFT_4_SEQUENCE=CCATTCTCTCTTTTCCATTCCA
PRIMER_RIGHT_4_SEQUENCE=CCACCGGAAACGATACTAAGAG
PRIMER_LEFT_4=192,22
PRIMER_RIGHT_4=428,22
PRIMER_LEFT_4_TM=60.423
PRIMER_RIGHT_4_TM=60.015
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_03476 Ps_contig_mira-and-tgicl-ass_franssen_30100
SEQUENCE=TCTTTATTAAAGACACCCACTACTCTCTCCATTCTCGYGGGGAACACACAACACAACAACCATTCCTCTCTATTCTCTTCTCCGATCATGTTGAGGAAAAGACCCTCTCCAATGATCGGAAAATTGTCGGAACTCTTGGTCTCCGGCCACCGTATCATGAACACCACCGGAAGCCCAAGAGGCCCATTAGACACAAAGATGCAATCATCATCACCGAGAGGTTTAAAAAACTACGACCTCAGTAGCGTTGGTCTCAAGATCGTTGCAGCTCTCGATAGCAGCAACAAAACATGTGAAGTTTTGCCTAAACACGCTGTTTGTACATCGAATTTGAATCGATCAAGACCGATCCAAATTCAATCTGCGAAAAACGCAAACGGGTTTCAAATGGATTATTCAAGTATGGAAGAAGATGAAGAATACACATATGTCACATGCCATGTACCTAATAAAACCTTCACTAAGGTTTATTACGACGGTGAAGTTCGGAGACAACAAGGTCACAACAACAACAACAACGACGTTGGTGTTGTAAGAAGATCCGAACCGGAACCGGAACCGGAACCATTATTTCCAACCTCAAATTTTCTTAATTCATGTCACTTATGCGGAAAGAATCTACATGGAAAAGATATATACATGTATAGAGGTGAGAAAGGGTTTTGTAGCACAGAGTGTCGTTCGAGTCAAATAATGATGGATGAACGAAAAGAGAGGTGTGGTTCAGAGGCTGTGGAATTGTCGAGTTCACCATATACAAGGGGTCAAATATTTTCAACTGGAATTCTTGCGGTTTAACTATTGATTAATTAGTATCTAAATCTGGATTAAGAAAATCGATAGATACTGTTAAGTAAATAATAATAATAATAATAATATATATACGGCTATACTATGGTTAACTGTATGAATATTTATATGAGAATTAAGATAGATATAGCTCGATCGTAACCAAAACGAAAATTTTGATGAGAAATAATGAGAGAGTGGATTATTTTGATGTAATGATATTATGCCCAAGGAATTATGAATTATGAACTCGAAATCTCAATTGCTTAGATCGATTTGTGTTGTTTATGAATTCTGTGTCAAACATAAACTAATTGGAAATATTTAAAGAANTATCTTGGTCAATGTTTTTCATTATGCTTCTAAATGAAAATATTTTTCAATT
TARGET=503,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03477 Ps_contig_mira-and-tgicl-ass_franssen_30100
SEQUENCE=TCTTTATTAAAGACACCCACTACTCTCTCCATTCTCGYGGGGAACACACAACACAACAACCATTCCTCTCTATTCTCTTCTCCGATCATGTTGAGGAAAAGACCCTCTCCAATGATCGGAAAATTGTCGGAACTCTTGGTCTCCGGCCACCGTATCATGAACACCACCGGAAGCCCAAGAGGCCCATTAGACACAAAGATGCAATCATCATCACCGAGAGGTTTAAAAAACTACGACCTCAGTAGCGTTGGTCTCAAGATCGTTGCAGCTCTCGATAGCAGCAACAAAACATGTGAAGTTTTGCCTAAACACGCTGTTTGTACATCGAATTTGAATCGATCAAGACCGATCCAAATTCAATCTGCGAAAAACGCAAACGGGTTTCAAATGGATTATTCAAGTATGGAAGAAGATGAAGAATACACATATGTCACATGCCATGTACCTAATAAAACCTTCACTAAGGTTTATTACGACGGTGAAGTTCGGAGACAACAAGGTCACAACAACAACAACAACGACGTTGGTGTTGTAAGAAGATCCGAACCGGAACCGGAACCGGAACCATTATTTCCAACCTCAAATTTTCTTAATTCATGTCACTTATGCGGAAAGAATCTACATGGAAAAGATATATACATGTATAGAGGTGAGAAAGGGTTTTGTAGCACAGAGTGTCGTTCGAGTCAAATAATGATGGATGAACGAAAAGAGAGGTGTGGTTCAGAGGCTGTGGAATTGTCGAGTTCACCATATACAAGGGGTCAAATATTTTCAACTGGAATTCTTGCGGTTTAACTATTGATTAATTAGTATCTAAATCTGGATTAAGAAAATCGATAGATACTGTTAAGTAAATAATAATAATAATAATAATATATATACGGCTATACTATGGTTAACTGTATGAATATTTATATGAGAATTAAGATAGATATAGCTCGATCGTAACCAAAACGAAAATTTTGATGAGAAATAATGAGAGAGTGGATTATTTTGATGTAATGATATTATGCCCAAGGAATTATGAATTATGAACTCGAAATCTCAATTGCTTAGATCGATTTGTGTTGTTTATGAATTCTGTGTCAAACATAAACTAATTGGAAATATTTAAAGAANTATCTTGGTCAATGTTTTTCATTATGCTTCTAAATGAAAATATTTTTCAATT
TARGET=857,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03478 Ps_contig_mira-and-tgicl-ass_franssen_30102
SEQUENCE=CTCTTTCTTTCTCNACTTCTATATAGCTAAACCAAGCTACATTGTTAAGAAGAGAACATACACACACACCCTTAAGGAGAAACTAACAAATATTTTCAGTTTGAGAGAGAAGGGAAAGTTAAAAGAGTGTTTGGATTTCAGTGAGAAGCCAAAAATGGATACTTTGATTGGAAAGGAGAATGGTTTGAACATGAGGGAGACTGAGCTTTGTCTTGGTTTGCCTGGCGGAGGCGGCGGCGGCGGCGAGGTTGAAACTCCTAGAGCTGCTGGAAAGAGAGGTTTCTCTGAGACAGTTGATTTGAAGCTGAATATTCAGACCAAGGAAGATCTGAATGAGAATCTGAAGAATGTTTCAAAGGAGAAGAGCATGCTTAAGGACCCTGCCAAGCCACCAGCTAAGACTCAAGTAGTTGGTTGGCCACCAGTGAGGTCATACAGAAAGAACATGATGGCACAAAAGGTTAACAATTCTGAGGAGAATGAGAAGACAACAAGTAGCACTACTGCTGGTGCATTTGTTAAGGTTTCCATGGATGGAGCACCTTACCTTCGTAAGGTCGACTTAACAATGTACAAAACCTACAAAGATTTATCCGATGCCTTAGCCAAAATGTTCAGCTCCTTCACCACTGGTAACTATGGGGCCCAAGGAATGATAGACTTCATGAATGAAAGCAAGTTGATGGATCTTCTTAACAGCTCTGAGTATGTGCCAACCTATGAAGATAAGGATGGCGACTGGATGCTCGTGGGAGATGTCCCATGGGAGATGTTTGTTGAATCATGCAAGCGTCTTCGCATAATGAAAGGATCAGAAGCTATTGGGCTTGCACCAAGAGCAATGGAGAAATGCAAAAACAGAAGCTAAATACATATCTTATATGAAAGTCCTGTACCAAAGTGGACCTATAAGAAAAGATATATATGAAGCTAGCACTACTTGTGGATTATTGGAGTGTTGTTAGTTTGGATGTGTCTTCAGTGTTTTTTGTTTTGTTCGTATATAGATTCAACTTTAATTTTCAAGTATTTGTAATCGACATGAAGCAATAAACTTAACCAAACCACCTATATACTATATAGTTTGCCTTGTTTAATTATTACTATTATTATTACTATTATGTGATTGCTTCCATGCTGTCATGTTTGTTGTGTGTGCTATATATTATATATACTTTGAATTTTAATGTATAGATTCTTCTTGTTATAT
TARGET=60,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03479 Ps_contig_mira-and-tgicl-ass_franssen_30102
SEQUENCE=CTCTTTCTTTCTCNACTTCTATATAGCTAAACCAAGCTACATTGTTAAGAAGAGAACATACACACACACCCTTAAGGAGAAACTAACAAATATTTTCAGTTTGAGAGAGAAGGGAAAGTTAAAAGAGTGTTTGGATTTCAGTGAGAAGCCAAAAATGGATACTTTGATTGGAAAGGAGAATGGTTTGAACATGAGGGAGACTGAGCTTTGTCTTGGTTTGCCTGGCGGAGGCGGCGGCGGCGGCGAGGTTGAAACTCCTAGAGCTGCTGGAAAGAGAGGTTTCTCTGAGACAGTTGATTTGAAGCTGAATATTCAGACCAAGGAAGATCTGAATGAGAATCTGAAGAATGTTTCAAAGGAGAAGAGCATGCTTAAGGACCCTGCCAAGCCACCAGCTAAGACTCAAGTAGTTGGTTGGCCACCAGTGAGGTCATACAGAAAGAACATGATGGCACAAAAGGTTAACAATTCTGAGGAGAATGAGAAGACAACAAGTAGCACTACTGCTGGTGCATTTGTTAAGGTTTCCATGGATGGAGCACCTTACCTTCGTAAGGTCGACTTAACAATGTACAAAACCTACAAAGATTTATCCGATGCCTTAGCCAAAATGTTCAGCTCCTTCACCACTGGTAACTATGGGGCCCAAGGAATGATAGACTTCATGAATGAAAGCAAGTTGATGGATCTTCTTAACAGCTCTGAGTATGTGCCAACCTATGAAGATAAGGATGGCGACTGGATGCTCGTGGGAGATGTCCCATGGGAGATGTTTGTTGAATCATGCAAGCGTCTTCGCATAATGAAAGGATCAGAAGCTATTGGGCTTGCACCAAGAGCAATGGAGAAATGCAAAAACAGAAGCTAAATACATATCTTATATGAAAGTCCTGTACCAAAGTGGACCTATAAGAAAAGATATATATGAAGCTAGCACTACTTGTGGATTATTGGAGTGTTGTTAGTTTGGATGTGTCTTCAGTGTTTTTTGTTTTGTTCGTATATAGATTCAACTTTAATTTTCAAGTATTTGTAATCGACATGAAGCAATAAACTTAACCAAACCACCTATATACTATATAGTTTGCCTTGTTTAATTATTACTATTATTATTACTATTATGTGATTGCTTCCATGCTGTCATGTTTGTTGTGTGTGCTATATATTATATATACTTTGAATTTTAATGTATAGATTCTTCTTGTTATAT
TARGET=230,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0892
PRIMER_LEFT_PENALTY=0.061184
PRIMER_RIGHT_PENALTY=0.028056
PRIMER_LEFT_SEQUENCE=CTTTGATTGGAAAGGAGAATGG
PRIMER_RIGHT_SEQUENCE=TTCTGTATGACCTCACTGGTGG
PRIMER_LEFT=161,22
PRIMER_RIGHT=439,22
PRIMER_LEFT_TM=59.939
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.1054
PRIMER_LEFT_1_PENALTY=0.077340
PRIMER_RIGHT_1_PENALTY=0.028056
PRIMER_LEFT_1_SEQUENCE=GGAGACTGAGCTTTGTCTTGGT
PRIMER_RIGHT_1_SEQUENCE=TTCTGTATGACCTCACTGGTGG
PRIMER_LEFT_1=195,22
PRIMER_RIGHT_1=439,22
PRIMER_LEFT_1_TM=59.923
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.1360
PRIMER_LEFT_2_PENALTY=0.061184
PRIMER_RIGHT_2_PENALTY=0.074807
PRIMER_LEFT_2_SEQUENCE=CTTTGATTGGAAAGGAGAATGG
PRIMER_RIGHT_2_SEQUENCE=CTTGAGTCTTAGCTGGTGGCTT
PRIMER_LEFT_2=161,22
PRIMER_RIGHT_2=406,22
PRIMER_LEFT_2_TM=59.939
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=0.1432
PRIMER_LEFT_3_PENALTY=0.115189
PRIMER_RIGHT_3_PENALTY=0.028056
PRIMER_LEFT_3_SEQUENCE=GAACATGAGGGAGACTGAGCTT
PRIMER_RIGHT_3_SEQUENCE=TTCTGTATGACCTCACTGGTGG
PRIMER_LEFT_3=186,22
PRIMER_RIGHT_3=439,22
PRIMER_LEFT_3_TM=59.885
PRIMER_RIGHT_3_TM=60.028
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.1521
PRIMER_LEFT_4_PENALTY=0.077340
PRIMER_RIGHT_4_PENALTY=0.074807
PRIMER_LEFT_4_SEQUENCE=GGAGACTGAGCTTTGTCTTGGT
PRIMER_RIGHT_4_SEQUENCE=CTTGAGTCTTAGCTGGTGGCTT
PRIMER_LEFT_4=195,22
PRIMER_RIGHT_4=406,22
PRIMER_LEFT_4_TM=59.923
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_03480 Ps_contig_mira-and-tgicl-ass_franssen_30115
SEQUENCE=TGGGGGATTAAAAAACAAAAAGAAAAGAACCACTCTCAAGTTAGAAACATCTGTGTGCTGTATCCATCTACATCTCTCTTCCTCTTTATACTTCCATTTCACCTTATATACCCTCTTCTCCATTCTCATTCTTCCTACACCATCACCACATTCGTCTTCTCTTTCTCTCTCTCTCAATTCCTCCTTTTCAGGAATTTGGAGAGAGTAGTGATTAACCAAACATGAAGTTTTCAATGATTTTTCTTGTTAGCAACATTCTATTGCTTCTAAGTTCTTCACCAGCATTTGCCAAAACTGTAAGTCCACCGGCACCGATCGCGCCGACACCGACTCCAACTCCGGCCCCAGCACCTGCACCAGATTATGTAAACCTAACTGAACTACTCGATGTTGCTGGTCCATTTCACACTTTCCTTGGATACCTTGAATCCACAAAAGTGTTGGACACATTCCAAAACCAAGCAAACAACACAGAAGAAGGAATCACTATCTTTGTACCAAAAGACACTGCCTTCAATTCATTGAAGAAACCTTCTTTATCCAAACTCAAAGATGATCAAATAAAACAAGTGATCCTTTTCCATGCATTGCCGCATTTCTATTCGCTTTCCGATTTCAAAAACCTTAGCCAGACTAGCCTAACTCCCACGTTCGCCGGAGGCGATTACACACTGAATTTCACCGATGATTCAGGAACCATTCGCATTAACTCGGGATGGTCGATTACTAAAGTAACAAGTGCTGTTCATGCAACTAACCCTGTTGCAATTTATCAAGTTGATAAGGTCTTACTTCCCGAGGCTATTTTCGGAACTGATATTCCACCTGCACCGGCTCCGGCTCCTACACCAGAGATCGCGCCAGCAGCGGATTCTCCGACTGAGAAAAGTGCTGACAGTAAATCATCGTCGCCTTCGTCTTCGCCAGACGGTTCTTCTGCTTACAAGTTAGTCAGCTATGGAATTTGGAGCAACATTGTGTTGGCAAGTTGTGGTTTGGTGATGATGTTGTTGTGAAGATGAAGATGAATTTGTGGAGTATTTAATTTAGATTTATTGTGTTGATGATGTATCTACTTTTGATGATGATATTTGCTTTGATTTGAATCCATTTTTTTGTGAACATAATGTGTTATTCAATATATGTATGAGAATGTGGTTTCTATTGAACTACTTTTTTTTAAATAAATAACTAGTAGCATGTTATTGTCATGTTATACTATGATGTTATTACCAATGCCTATTTTCTACTCTAAATTTNTCTTAAACTTAAAATT
TARGET=164,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2114
PRIMER_LEFT_PENALTY=0.147295
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=CATTCTTCCTACACCATCACCA
PRIMER_RIGHT_SEQUENCE=CCAGCAACATCGAGTAGTTCAG
PRIMER_LEFT=126,22
PRIMER_RIGHT=396,22
PRIMER_LEFT_TM=59.853
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.3444
PRIMER_LEFT_1_PENALTY=0.147295
PRIMER_RIGHT_1_PENALTY=0.197140
PRIMER_LEFT_1_SEQUENCE=CATTCTTCCTACACCATCACCA
PRIMER_RIGHT_1_SEQUENCE=ACCAGCAACATCGAGTAGTTCA
PRIMER_LEFT_1=126,22
PRIMER_RIGHT_1=397,22
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.3470
PRIMER_LEFT_2_PENALTY=0.282868
PRIMER_RIGHT_2_PENALTY=0.064105
PRIMER_LEFT_2_SEQUENCE=TTCCTACACCATCACCACATTC
PRIMER_RIGHT_2_SEQUENCE=CCAGCAACATCGAGTAGTTCAG
PRIMER_LEFT_2=131,22
PRIMER_RIGHT_2=396,22
PRIMER_LEFT_2_TM=59.717
PRIMER_RIGHT_2_TM=59.936
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.3630
PRIMER_LEFT_3_PENALTY=0.298898
PRIMER_RIGHT_3_PENALTY=0.064105
PRIMER_LEFT_3_SEQUENCE=TACACCATCACCACATTCGTCT
PRIMER_RIGHT_3_SEQUENCE=CCAGCAACATCGAGTAGTTCAG
PRIMER_LEFT_3=135,22
PRIMER_RIGHT_3=396,22
PRIMER_LEFT_3_TM=60.299
PRIMER_RIGHT_3_TM=59.936
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.3630
PRIMER_LEFT_4_PENALTY=0.298898
PRIMER_RIGHT_4_PENALTY=0.064105
PRIMER_LEFT_4_SEQUENCE=CTACACCATCACCACATTCGTC
PRIMER_RIGHT_4_SEQUENCE=CCAGCAACATCGAGTAGTTCAG
PRIMER_LEFT_4=134,22
PRIMER_RIGHT_4=396,22
PRIMER_LEFT_4_TM=60.299
PRIMER_RIGHT_4_TM=59.936
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_03481 Ps_contig_mira-and-tgicl-ass_franssen_30123
SEQUENCE=GGGCGTTTTGTTCATTGAAGTTGACGACGAATTGAATCATCATCATTCATCACCACTCAATCCCCATTCTCCTCTCTTCTCTCTTCTCTCTCCTCTTCTAATCTCATCTCATCTATCCCACACAGATTCCCCTCCTTTTCCCAGAAACTAAATAATTACATTAATAAGTTATTATTATTATTCAAATTAAATAAAATTAAATTAATAAATAAAGGGAAACCCCTCTTTTTCCCTTCTCGATTCTCATTCGTTTAATTTAACCAAACCCCCACTCCTTATTTGTTTTCCTTCAACATCAAAGCGACTCGATTTCCCAAAAATTTTTCTGGGAACCTAGGGCACAAATATGGTTTCCCGGGAAAACAACAATTGGCTTTTCGATTACGGCTTAATCGACGATATTTCCCCTCCCATTTCCACCTTCATGCCCACCCCTTCCCCCGGCTTCACCTGGCCCACACACCAGCCATTCAATGTTTCTTCCAATGTCGGTGCTGAAGCTGATGTCTCGTTAGGGGATTCTGACAGTCTCAAAGAGTCTGGTTCAAAGAAGAGGGGTAGATCTGAGTCATGTGCTGCCACTAGCTCCAAGGCATGTCGGGAGAAGTTGCGTAGAGATAGGCTTAATGACAAGTTTATTGAATTGGGCTCCATTTTGGAGCCTGGAAGGCCTGCCAAAACAGACAAGGCTGCTATCCTGATGGATGCTGTCCGAATGGTGACACAGTTGAAGGGTGAAGCCGAAAAGTTGAAAGATGCTAATACGGGTCTTCAAGAAAAGATTAAAGAATTGAAGGTTGAGAAGAATGAACTCCGTGACGAGAAACAGAGGCTCAAGGCAGAGAAAGAGAAGTTGGAGCAGCAGCTGAAATCTATGAATGCACAACCCAGTTTCTTGCCTCCCCCCACTGCACTCCCTGCTGCACTACCTGCTGCATTTGCTGCACAAGGCCAAGCCCATGGCAACAAGTTGGTACCTTTTATTAGTTATCCAGGAGTTGCAATGTGGCAATTTATGCCACCTGCTGCAGTGGATACCTCACAGGATCATGTACTTCGTCCTCCAGTTGCCTAAACTGGCACTGTGCAATTCTTTGGGCTTCCATTTTGGCTCAAGATTGTATCCAATCACCGATACCTTTTTGGCTTTGTTAGTTTTCTCTGTATTCTAACTGGACCCAGTGGCTTCCCTCTGTATTTTGCATATGGATACTATTATTNAATTAATGCTGGTATCTTCCTGAATAATAATAACCTATATTAC
TARGET=169,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1690
PRIMER_LEFT_PENALTY=0.154023
PRIMER_RIGHT_PENALTY=0.014945
PRIMER_LEFT_SEQUENCE=GTTCATTGAAGTTGACGACGAA
PRIMER_RIGHT_SEQUENCE=AAAACAAATAAGGAGTGGGGGT
PRIMER_LEFT=9,22
PRIMER_RIGHT=285,22
PRIMER_LEFT_TM=60.154
PRIMER_RIGHT_TM=59.985
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=10.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=277
PRIMER_PAIR_PENALTY_1=0.1690
PRIMER_LEFT_1_PENALTY=0.154023
PRIMER_RIGHT_1_PENALTY=0.014945
PRIMER_LEFT_1_SEQUENCE=GTTCATTGAAGTTGACGACGAA
PRIMER_RIGHT_1_SEQUENCE=AACAAATAAGGAGTGGGGGTTT
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=283,22
PRIMER_LEFT_1_TM=60.154
PRIMER_RIGHT_1_TM=59.985
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.1690
PRIMER_LEFT_2_PENALTY=0.154023
PRIMER_RIGHT_2_PENALTY=0.014945
PRIMER_LEFT_2_SEQUENCE=GTTCATTGAAGTTGACGACGAA
PRIMER_RIGHT_2_SEQUENCE=AAACAAATAAGGAGTGGGGGTT
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=284,22
PRIMER_LEFT_2_TM=60.154
PRIMER_RIGHT_2_TM=59.985
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.2105
PRIMER_LEFT_3_PENALTY=0.039531
PRIMER_RIGHT_3_PENALTY=0.170958
PRIMER_LEFT_3_SEQUENCE=ATCCCCATTCTCCTCTCTTCTC
PRIMER_RIGHT_3_SEQUENCE=GAAAAAGAGGGGTTTCCCTTTA
PRIMER_LEFT_3=59,22
PRIMER_RIGHT_3=230,22
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=59.829
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.3246
PRIMER_LEFT_4_PENALTY=0.154023
PRIMER_RIGHT_4_PENALTY=0.170538
PRIMER_LEFT_4_SEQUENCE=GTTCATTGAAGTTGACGACGAA
PRIMER_RIGHT_4_SEQUENCE=TGAGAATCGAGAAGGGAAAAAG
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=245,22
PRIMER_LEFT_4_TM=60.154
PRIMER_RIGHT_4_TM=59.829
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_03482 Ps_contig_mira-and-tgicl-ass_franssen_30124
SEQUENCE=ACTTTTTTTRNTCAACCCCCAAACGTGCTTTTCATTTTTGAACCGAGTCCTTCTTCAATCATCACTCTCTCTCTGTCTCACAAAATAAAACAATAATCACTTATTTATAAAAAAATTAAAAACAAAATTATCACCGCCGGGAAAATCTCACCGAACAATGGTTTCCCCGGAAAACACCAATTGGCTTTTCGATTATGCTTTGATTGACGATATTCCCGTCTCCGATGGCTCCTTCGCCTTCACCTGGCCCCCATCACATCCTCTCAATGTCTGTGTTGATATGGATGGTTCTCTGGGAGATTCTGATGGTATCAAAGATCCTGGTTCAAAGAAGAGAGGTAGATCAGATTCGTCTGCTCCTTCCAGCTCTAAGGCATGTCGAGAGAAGCTGCGGAGGGATAGGCTGAACGATAAGTTTGTTGAATTAGGCTCTATCTTGGAGCCTGGAAGGCCACCAAAAACAGACAAGGCAGCAATTCTGACTGATGCTGTCCGAATGGTATCACAGCTACGCGGTGAAGCCCAGAAGTTGAAAGACTCCAATTCAAGTCTTCAAGAAAAGATTAAAGAATTAAAGGTTGAGAAGAATGAACTCCGTGATGAGAAGCAGAGGCTTAAGGCTGAGAAAGAGAAGCTGGAGCAGCAGGTGAAATCAATGAATGCCCAACCCAGTTTCTTGACACACCCTCCTGCAATCCCTGCTGCATTTGCTCCACAAGGCCAAGCCCATAGCAACAAACTAATGCCTTTCATGAGTTATCCAGGAGTTGCCATGTGGCAATTCATGCCACCAGCCTCTGTGGATACCTCACAGGATCATGTGCTCCGTCCACCAGTTGCCTAAGTTGGCACTGTACAATTTTTTGGGATTCCATTATGGCCTAAAAATATTGTATTCCTTCCCCATACGCCCTTTCCTTTTAGTTATTTGCTATGTATTCTAACTGGTATTTGCTATTGTGAGGTCTAATACTATTATGAAGTAATGCTGGTATTTGCATGTAAATCTTATAAGCTCGT
TARGET=65,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03483 Ps_contig_mira-and-tgicl-ass_franssen_30125
SEQUENCE=TTGTAAGCAAAATATAAATTCAAAACTTTATATTTAATCATACAAAAATGCTAAAAAAGTTGACAACTACTAGTAATTCCTCAACTGTACAATATTAACTCAAAATAACCTAAAAAAACGGTCAGAATTAAAAAAAACGAAGCTCAAGTTTACAGTTAGCTAAAAACAACCGAAACAATGATACTCAAAACGACAAATGAATTAAACACAAAACGACATAAACCCGAATCTACTGCCGCAACTCTTTTCGGAGTTTCTGAGAGAATGATTTGCACGCATCCATGCTCAAATCCATGGCAGCTGCAAGTGCAACAAACGCCGCAGCATCCTCCGCACAAGTCACGTGCTTCACACCAACCTCCACCTCCGGTTTACTACACTTTTTTTCTCCGTCAAACCTCGCTGACATCACAAACCCTCGGTAGAAAAACTGATTCTCTAGTGAGAAATCCGAACCGGATCCAGATCCAGGTCTTGAACTCGTTCCTGATCCGAGATCCCAGCTTCCATGTGGAGTACTCGCCGGAGTAACACCGGGCGTAACATCAATAGTGAATTTACCTCCGTGCTGTGAATTAATAGTACACGCGGCGAGAGTAACAGGATCAGCGGTGGCCGGAAGAACGTCGAAACGGTAACCGACGTCGTTTGAGTTACCAGGCTCACGCCAAGCCTCGAGCCGACCCCATGGCTTCCATGTACCATCACCATCGGGTCGGATAATAAGCCAAGCTCCCGGGTTGGATTTACTGACCCGTTGTGAACCGGGTGAGGGAACAAACGGAGTAACCATCGACGCACACGCAACCGGTGAACCGGATAAATCGTGTATCGTAATAGACCATCCTTTTCGATCCGCTACCATAGGATGATTAGCAGCATGAAATTGAACCGGGTTTCTATCTCTGAAACTGAACTTGCAAGTGAAAACTGGTTGTTTAACGTCTCCTTTGACTTGGAAAACCTGTGGACTACACTCTGGTTCACCGTCGAACCGAAAAATGAATCGCGGATCCGGTTCGGACCGGATAGTTAAATGCAATAACTGAGCTTGAGTTTGAGTTTCGGTTTTGTTGTTGTTGCTTTTGGTTAGAGCAACCCAACCGTTATGAAAAGAGCAAGGTCGTGATTCCGATAAGGTGAGATCTAGTGGAATTGAAATTTGACCTAATAATTTGGAGGAATTGAAAACGCATGAAGGATTTGTTCGTGTTTTGTAGACGGAGATTTTGATGAAAGGGTTTTTAATATTGGAGAGTTGCGATTTGGGGAAATCGAAGCTAGCGGCGAGAGAGTGAGGGTGAGTTTCTGATTCTGAAATTAGAGGAACGGTTGCCACGTGGCACGGTGGTGAATCGACGCCTCTGAGTTTGATTTTGCAGTAGCATGGCGAACTGGAAGGGTGAACTTTGCCGGAGAAAGATGGATTTGAGGATGCCGGAGATTTGATTGCTAGGTTTCCGACGAGGACCCGTACGAAGGAGCACGGATCCATTGATTAGGGTTTGGGAGAATTGAAATGAAATGAAATTGAAATTTGAATCCCCGANTGAGAG
TARGET=1071,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0798
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.067678
PRIMER_LEFT_SEQUENCE=TCCTTTGACTTGGAAAACCTGT
PRIMER_RIGHT_SEQUENCE=CCGTCTACAAAACACGAACAAA
PRIMER_LEFT=946,22
PRIMER_RIGHT=1223,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.068
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=278
PRIMER_PAIR_PENALTY_1=0.1294
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.117281
PRIMER_LEFT_1_SEQUENCE=TCCTTTGACTTGGAAAACCTGT
PRIMER_RIGHT_1_SEQUENCE=TCACGACCTTGCTCTTTTCATA
PRIMER_LEFT_1=946,22
PRIMER_RIGHT_1=1128,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.1413
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=TCCTTTGACTTGGAAAACCTGT
PRIMER_RIGHT_2_SEQUENCE=GACCTTGCTCTTTTCATAACGG
PRIMER_LEFT_2=946,22
PRIMER_RIGHT_2=1124,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.1496
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=0.137489
PRIMER_LEFT_3_SEQUENCE=TCCTTTGACTTGGAAAACCTGT
PRIMER_RIGHT_3_SEQUENCE=ATCACGACCTTGCTCTTTTCAT
PRIMER_LEFT_3=946,22
PRIMER_RIGHT_3=1129,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=60.137
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.2046
PRIMER_LEFT_4_PENALTY=0.136881
PRIMER_RIGHT_4_PENALTY=0.067678
PRIMER_LEFT_4_SEQUENCE=CTCCTTTGACTTGGAAAACCTG
PRIMER_RIGHT_4_SEQUENCE=CCGTCTACAAAACACGAACAAA
PRIMER_LEFT_4=945,22
PRIMER_RIGHT_4=1223,22
PRIMER_LEFT_4_TM=60.137
PRIMER_RIGHT_4_TM=60.068
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=279
=
PRIMER_SEQUENCE_ID=SSR_03484 Ps_contig_mira-and-tgicl-ass_franssen_30125
SEQUENCE=TTGTAAGCAAAATATAAATTCAAAACTTTATATTTAATCATACAAAAATGCTAAAAAAGTTGACAACTACTAGTAATTCCTCAACTGTACAATATTAACTCAAAATAACCTAAAAAAACGGTCAGAATTAAAAAAAACGAAGCTCAAGTTTACAGTTAGCTAAAAACAACCGAAACAATGATACTCAAAACGACAAATGAATTAAACACAAAACGACATAAACCCGAATCTACTGCCGCAACTCTTTTCGGAGTTTCTGAGAGAATGATTTGCACGCATCCATGCTCAAATCCATGGCAGCTGCAAGTGCAACAAACGCCGCAGCATCCTCCGCACAAGTCACGTGCTTCACACCAACCTCCACCTCCGGTTTACTACACTTTTTTTCTCCGTCAAACCTCGCTGACATCACAAACCCTCGGTAGAAAAACTGATTCTCTAGTGAGAAATCCGAACCGGATCCAGATCCAGGTCTTGAACTCGTTCCTGATCCGAGATCCCAGCTTCCATGTGGAGTACTCGCCGGAGTAACACCGGGCGTAACATCAATAGTGAATTTACCTCCGTGCTGTGAATTAATAGTACACGCGGCGAGAGTAACAGGATCAGCGGTGGCCGGAAGAACGTCGAAACGGTAACCGACGTCGTTTGAGTTACCAGGCTCACGCCAAGCCTCGAGCCGACCCCATGGCTTCCATGTACCATCACCATCGGGTCGGATAATAAGCCAAGCTCCCGGGTTGGATTTACTGACCCGTTGTGAACCGGGTGAGGGAACAAACGGAGTAACCATCGACGCACACGCAACCGGTGAACCGGATAAATCGTGTATCGTAATAGACCATCCTTTTCGATCCGCTACCATAGGATGATTAGCAGCATGAAATTGAACCGGGTTTCTATCTCTGAAACTGAACTTGCAAGTGAAAACTGGTTGTTTAACGTCTCCTTTGACTTGGAAAACCTGTGGACTACACTCTGGTTCACCGTCGAACCGAAAAATGAATCGCGGATCCGGTTCGGACCGGATAGTTAAATGCAATAACTGAGCTTGAGTTTGAGTTTCGGTTTTGTTGTTGTTGCTTTTGGTTAGAGCAACCCAACCGTTATGAAAAGAGCAAGGTCGTGATTCCGATAAGGTGAGATCTAGTGGAATTGAAATTTGACCTAATAATTTGGAGGAATTGAAAACGCATGAAGGATTTGTTCGTGTTTTGTAGACGGAGATTTTGATGAAAGGGTTTTTAATATTGGAGAGTTGCGATTTGGGGAAATCGAAGCTAGCGGCGAGAGAGTGAGGGTGAGTTTCTGATTCTGAAATTAGAGGAACGGTTGCCACGTGGCACGGTGGTGAATCGACGCCTCTGAGTTTGATTTTGCAGTAGCATGGCGAACTGGAAGGGTGAACTTTGCCGGAGAAAGATGGATTTGAGGATGCCGGAGATTTGATTGCTAGGTTTCCGACGAGGACCCGTACGAAGGAGCACGGATCCATTGATTAGGGTTTGGGAGAATTGAAATGAAATGAAATTGAAATTTGAATCCCCGANTGAGAG
TARGET=1516,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.4435
PRIMER_LEFT_PENALTY=0.347645
PRIMER_RIGHT_PENALTY=2.095861
PRIMER_LEFT_SEQUENCE=GTTTGATTTTGCAGTAGCATGG
PRIMER_RIGHT_SEQUENCE=CTCTCANTCGGGGATTCAAA
PRIMER_LEFT=1368,22
PRIMER_RIGHT=1555,20
PRIMER_LEFT_TM=59.652
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.368
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.50
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.75
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=2.4657
PRIMER_LEFT_1_PENALTY=0.369874
PRIMER_RIGHT_1_PENALTY=2.095861
PRIMER_LEFT_1_SEQUENCE=AGATTTGATTGCTAGGTTTCCG
PRIMER_RIGHT_1_SEQUENCE=CTCTCANTCGGGGATTCAAA
PRIMER_LEFT_1=1441,22
PRIMER_RIGHT_1=1555,20
PRIMER_LEFT_1_TM=59.630
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.368
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.50
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=2.4657
PRIMER_LEFT_2_PENALTY=0.369874
PRIMER_RIGHT_2_PENALTY=2.095861
PRIMER_LEFT_2_SEQUENCE=CGGAGATTTGATTGCTAGGTTT
PRIMER_RIGHT_2_SEQUENCE=CTCTCANTCGGGGATTCAAA
PRIMER_LEFT_2=1438,22
PRIMER_RIGHT_2=1555,20
PRIMER_LEFT_2_TM=59.630
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.368
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.50
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=2.5225
PRIMER_LEFT_3_PENALTY=0.426682
PRIMER_RIGHT_3_PENALTY=2.095861
PRIMER_LEFT_3_SEQUENCE=CGAGAGAGTGAGGGTGAGTTTC
PRIMER_RIGHT_3_SEQUENCE=CTCTCANTCGGGGATTCAAA
PRIMER_LEFT_3=1287,22
PRIMER_RIGHT_3=1555,20
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=47.368
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.50
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.75
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=2.5354
PRIMER_LEFT_4_PENALTY=0.439553
PRIMER_RIGHT_4_PENALTY=2.095861
PRIMER_LEFT_4_SEQUENCE=GATTTGATTGCTAGGTTTCCGA
PRIMER_RIGHT_4_SEQUENCE=CTCTCANTCGGGGATTCAAA
PRIMER_LEFT_4=1442,22
PRIMER_RIGHT_4=1555,20
PRIMER_LEFT_4_TM=60.440
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.368
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.50
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_4=114
=
PRIMER_SEQUENCE_ID=SSR_03485 Ps_contig_mira-and-tgicl-ass_franssen_30128
SEQUENCE=ACGACCTCTCTTTCTATCTTCTCCATTTTGCCACTCATTCCATAAAGAAACAAAATCATCATCATCAAGATATTAACCCTTTCATTCACCTTCTAAACTCTCATATGATAGTTCAATAAAAGTTCTAAACTTTGAGAGCCTCACAAAATTCTCTGTGAAATTCAAATTATGCTATGGAGGCCCTCAATTCTGCTGTTTTCACCCCCCTTTCTGTGCTTTCTGATAGAACAAAAAACCCCACAAAAATTTCATGCAAAGTTTCAAGCTTTTCCACTTCAATCACCAGAAAACACAACCTAAAAGAGTTTTTATCAAAGGGTTTACAAAAGGGTCTAATCCTTGCAGCAACTTCAGTTGTGAATGTTAATGTTAATCATGGTGAATTTGCCAAAGCTTTAACATATGAAGAAGCATTAGGACTATCTCCAAGTGCTTCAAACTATGGTGGTGACATTGATGTTAATGGTTTTGTAGAGAGTGTGGTAGGCTTTGCAACTGAGAACCCTGCAATTCTAGCTGGTGGAGTTGCTATTTTGGCAGTTCCATTGGTTTTGTCTCAGATTTTGAAGAAACCTAAGCCTTGGGGAGTTGAATCAGCTAAAAATGCTTATGCTAAATTGGGTGCTGATGGGAATGCTCAGTTGCTTGATATAAGAGGTTTGTCTGAGATTAGGCAAATTGGTGGCCCAAATGTGGGGGGTTTGAAGAAGAAATCGGTGTCCATAGCTTATAAAGGTGATGATAAGCCAGGGTTTTTGAAGAAGCTTTCTTTGAAATTTAAGGAACCAGAGAATACTACATTGTTCATTCTTGACAAATTTGACGGGAACTCTGAACTGGTTGCAGAACTAGTCACCCTTAATGGATTTAAGTCTGCTTATGCAATTAAGGATGGCGCAGAAGGACCACGAGGATGGGTGAATAGCGATCTTCCTTGGATTGAACCGAAGAAAGCATTGAGCTTTGATTTTGGAAGTCTGACAGATACTATCAATGATGCAATCGGAGAATCTAATGGCTTGGTTGTTACTCTTGGGATTGCTGCAGCTACTGGATTGGGTGCATTAGCTTTTTCTGAGGTAGAAACAATTCTCCAACTGGTAGGATCTGCTGCAATTGTTCAGTTTGCAAGCAAGAAGCTCCTATTTGCCGAGGACCGGAAGAAAACACTAAAACAAGTTGACGAGTTCTTGAACACCAAAGTTGCTCCTCAGGAGCTTGTTGATGAAATAAAGGACATCGGAAAGGCTCTTCTACCAACCTCCACCAACAGCGGTAAGGCTCTTCCTGCACCAACAGAAACTGTTCCAGAGCTCACTACAGCTAGTGCCACTGTACAGAAAGCAGAAGTTACTCCCGAAATTGTCGAGACCGTACAGAAAGCAGAAGCTACTCCTCCCGACGTTATCTCCGAGACCAAAGTAGAAGCAGCAGCAGAACCTGCTCCGGAAATAAACTCAGCCCCAAAAACTGAAACTGTGGCAGAATCAATTCCGGTGCAAGCAGCAGCAGAACCTGCTCCGGAAATAAACTCAGTCCCAAAAACTGAAACCGTGGCAGAATCAATTCCGGTGCAACCTAAACCGCTCTCACCGTACCCATATTATCCAGATCTCAAACCTCCAACATCTCCATCTCCTACACAGCCCTAGAGTGAAGAGAATCCTATAGCAGGCAAAATAGTTATCCAAAATATTCAGCATGTATTCTTGTTATTTGTGCTACTCAGTAGTCTTGTTTCTGTTATTGATCTCTGTGTTGTGGAGGTAGTTGTACTATTAGTTGTTATGTTGTGATTTAGTATCATATTACATCATTCAGTTTTGAATAAGCAAATAAGGTTTCAAATGTGCCTTTGTAATGATTCATGAAGTTTGGAATTAGTAAAGCATTGAACTTTTGGTTGTGTTC
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6074
PRIMER_LEFT_PENALTY=0.482784
PRIMER_RIGHT_PENALTY=0.124655
PRIMER_LEFT_SEQUENCE=CTTCTCCATTTTGCCACTCATT
PRIMER_RIGHT_SEQUENCE=GGTTGTGTTTTCTGGTGATTGA
PRIMER_LEFT=17,22
PRIMER_RIGHT=296,22
PRIMER_LEFT_TM=60.483
PRIMER_RIGHT_TM=59.875
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.7760
PRIMER_LEFT_1_PENALTY=0.482784
PRIMER_RIGHT_1_PENALTY=0.293254
PRIMER_LEFT_1_SEQUENCE=CTTCTCCATTTTGCCACTCATT
PRIMER_RIGHT_1_SEQUENCE=GGCCTCCATAGCATAATTTGAA
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=181,22
PRIMER_LEFT_1_TM=60.483
PRIMER_RIGHT_1_TM=60.293
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=1.0862
PRIMER_LEFT_2_PENALTY=0.482784
PRIMER_RIGHT_2_PENALTY=0.603423
PRIMER_LEFT_2_SEQUENCE=CTTCTCCATTTTGCCACTCATT
PRIMER_RIGHT_2_SEQUENCE=TATCAGAAAGCACAGAAAGGGG
PRIMER_LEFT_2=17,22
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=60.483
PRIMER_RIGHT_2_TM=60.603
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=1.1117
PRIMER_LEFT_3_PENALTY=0.987026
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=TCTTCTCCATTTTGCCACTCAT
PRIMER_RIGHT_3_SEQUENCE=GGTTGTGTTTTCTGGTGATTGA
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=60.987
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=1.1117
PRIMER_LEFT_4_PENALTY=0.987026
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=ATCTTCTCCATTTTGCCACTCA
PRIMER_RIGHT_4_SEQUENCE=GGTTGTGTTTTCTGGTGATTGA
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=296,22
PRIMER_LEFT_4_TM=60.987
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_03486 Ps_contig_mira-and-tgicl-ass_franssen_30131
SEQUENCE=CTTTCTTCCTANTTACATTAGATGAGGTTGGATTGGATCATTCATTCATGTAATAATTTGAATGTAAGAACATTTCTATATGTATACTACATTATACAAAAGAATGGTGTAATAGTACAGTTATGCAATACTTACCGACACAATAATGTTACAAGGCCAAAGAAAAATGCAGAATGCCAAACTTTGCACTTATTGATGTTGCGAGAATACTAATTTGAAAGCAAAATACCTGAGAACTGTTAAACATGAGAATCTTACATTCAAGGCCAATTTGTGAACTCAAACACTTTCCATACAATATCAAGTCCAGTGGGAATCTGCCATACGAAAAGAAGAACATTCAACGTATTCAGCGCGATGTGAAGATTTCTCGCTGTTTCACTCCCTTTCTGCATGGGTGGTACTAGCGCTGCAGCCAGTGCCCATAAGACCGTAATACCTGCTCCTGCGAATAAATGTGGACCGGGAAATAACTTTCCTGTCCTTAACCATGTGTTGAGTCCTCCACCAACAGCCTCAAAGACACCAAATCCTAGAAGTATGGATCCTGCATTGAAGTGTTTATCCCTATATGAACCTTTGATAAGCTCTTTCCTCTCCTCAGTAAGTTGCTGGATTTTGAGTTCAACAGGTGATGGTGGTGAAGTTTCCAGTGCTTTACCATCTGGGGTGACTGGAGCAGGCTTGACTTGTTTCTTGAGTTCATTGATTTCATTCTGAGTCGTCCTAACTCTTCGCCATTGCCATCCCAAATAGCCTGCATAAAGGGTATAAAAGAACAATCCACCCAACACAGCGGGGTGAATAAGAGCAAATGTTCTTCCTTCAAATATCCCAAACTCTCCACCAACAGCAAGTGCATCGTTCTGTTCCAGCAAGAACGGTAATGTGATTGCTGTGAGGGAAAGAGAAGTGTTTTTGAGAGTATTGATGTTTTGATCTAGAAACTTTTGTGGGGTGGGGACAATATTGAGTTTGGAAGATATGGATGGAAGTGGAACATGTTTTGAGGTTGTTTGGCGTGTCTTGATGGTTGGAAGAATGGGGAGCTTGAGAAGACTGAGTGTGGCGGCCATACTTTGCATATGGGGTTCAAACTGAGTGAGAGAGAGAGAGAGATTGAAGATCCTAGTACTACCTAGTAAAAGATAAGGTTCTTGTGCTTCTGTATTTTGAATTGCGGTCACTCCCCGT
TARGET=38,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3686
PRIMER_LEFT_PENALTY=1.096569
PRIMER_RIGHT_PENALTY=0.271999
PRIMER_LEFT_SEQUENCE=CATTAGATGAGGTTGGATTGGA
PRIMER_RIGHT_SEQUENCE=TTCTCGCAACATCAATAAGTGC
PRIMER_LEFT=15,22
PRIMER_RIGHT=206,22
PRIMER_LEFT_TM=58.903
PRIMER_RIGHT_TM=60.272
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=1.4892
PRIMER_LEFT_1_PENALTY=1.096569
PRIMER_RIGHT_1_PENALTY=0.392624
PRIMER_LEFT_1_SEQUENCE=CATTAGATGAGGTTGGATTGGA
PRIMER_RIGHT_1_SEQUENCE=GTTTGAGTTCACAAATTGGCCT
PRIMER_LEFT_1=15,22
PRIMER_RIGHT_1=284,22
PRIMER_LEFT_1_TM=58.903
PRIMER_RIGHT_1_TM=60.393
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=1.5748
PRIMER_LEFT_2_PENALTY=1.096569
PRIMER_RIGHT_2_PENALTY=0.478252
PRIMER_LEFT_2_SEQUENCE=CATTAGATGAGGTTGGATTGGA
PRIMER_RIGHT_2_SEQUENCE=GTGTTTGAGTTCACAAATTGGC
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=286,22
PRIMER_LEFT_2_TM=58.903
PRIMER_RIGHT_2_TM=59.522
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=1.7653
PRIMER_LEFT_3_PENALTY=1.096569
PRIMER_RIGHT_3_PENALTY=0.668761
PRIMER_LEFT_3_SEQUENCE=CATTAGATGAGGTTGGATTGGA
PRIMER_RIGHT_3_SEQUENCE=CGCAACATCAATAAGTGCAAAG
PRIMER_LEFT_3=15,22
PRIMER_RIGHT_3=202,22
PRIMER_LEFT_3_TM=58.903
PRIMER_RIGHT_3_TM=60.669
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=2.1997
PRIMER_LEFT_4_PENALTY=1.096569
PRIMER_RIGHT_4_PENALTY=1.103105
PRIMER_LEFT_4_SEQUENCE=CATTAGATGAGGTTGGATTGGA
PRIMER_RIGHT_4_SEQUENCE=GAGTTCACAAATTGGCCTTGA
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=280,21
PRIMER_LEFT_4_TM=58.903
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_03487 Ps_contig_mira-and-tgicl-ass_franssen_30131
SEQUENCE=CTTTCTTCCTANTTACATTAGATGAGGTTGGATTGGATCATTCATTCATGTAATAATTTGAATGTAAGAACATTTCTATATGTATACTACATTATACAAAAGAATGGTGTAATAGTACAGTTATGCAATACTTACCGACACAATAATGTTACAAGGCCAAAGAAAAATGCAGAATGCCAAACTTTGCACTTATTGATGTTGCGAGAATACTAATTTGAAAGCAAAATACCTGAGAACTGTTAAACATGAGAATCTTACATTCAAGGCCAATTTGTGAACTCAAACACTTTCCATACAATATCAAGTCCAGTGGGAATCTGCCATACGAAAAGAAGAACATTCAACGTATTCAGCGCGATGTGAAGATTTCTCGCTGTTTCACTCCCTTTCTGCATGGGTGGTACTAGCGCTGCAGCCAGTGCCCATAAGACCGTAATACCTGCTCCTGCGAATAAATGTGGACCGGGAAATAACTTTCCTGTCCTTAACCATGTGTTGAGTCCTCCACCAACAGCCTCAAAGACACCAAATCCTAGAAGTATGGATCCTGCATTGAAGTGTTTATCCCTATATGAACCTTTGATAAGCTCTTTCCTCTCCTCAGTAAGTTGCTGGATTTTGAGTTCAACAGGTGATGGTGGTGAAGTTTCCAGTGCTTTACCATCTGGGGTGACTGGAGCAGGCTTGACTTGTTTCTTGAGTTCATTGATTTCATTCTGAGTCGTCCTAACTCTTCGCCATTGCCATCCCAAATAGCCTGCATAAAGGGTATAAAAGAACAATCCACCCAACACAGCGGGGTGAATAAGAGCAAATGTTCTTCCTTCAAATATCCCAAACTCTCCACCAACAGCAAGTGCATCGTTCTGTTCCAGCAAGAACGGTAATGTGATTGCTGTGAGGGAAAGAGAAGTGTTTTTGAGAGTATTGATGTTTTGATCTAGAAACTTTTGTGGGGTGGGGACAATATTGAGTTTGGAAGATATGGATGGAAGTGGAACATGTTTTGAGGTTGTTTGGCGTGTCTTGATGGTTGGAAGAATGGGGAGCTTGAGAAGACTGAGTGTGGCGGCCATACTTTGCATATGGGGTTCAAACTGAGTGAGAGAGAGAGAGAGATTGAAGATCCTAGTACTACCTAGTAAAAGATAAGGTTCTTGTGCTTCTGTATTTTGAATTGCGGTCACTCCCCGT
TARGET=1104,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4897
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.481423
PRIMER_LEFT_SEQUENCE=TGGAAGTGGAACATGTTTTGAG
PRIMER_RIGHT_SEQUENCE=GTGACCGCAATTCAAAATACAG
PRIMER_LEFT=989,22
PRIMER_RIGHT=1186,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.519
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=0.5107
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.481423
PRIMER_LEFT_1_SEQUENCE=TGTCTTGATGGTTGGAAGAATG
PRIMER_RIGHT_1_SEQUENCE=GTGACCGCAATTCAAAATACAG
PRIMER_LEFT_1=1022,22
PRIMER_RIGHT_1=1186,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.519
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=0.5588
PRIMER_LEFT_2_PENALTY=0.077340
PRIMER_RIGHT_2_PENALTY=0.481423
PRIMER_LEFT_2_SEQUENCE=GCTGTGAGGGAAAGAGAAGTGT
PRIMER_RIGHT_2_SEQUENCE=GTGACCGCAATTCAAAATACAG
PRIMER_LEFT_2=894,22
PRIMER_RIGHT_2=1186,22
PRIMER_LEFT_2_TM=59.923
PRIMER_RIGHT_2_TM=59.519
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.5959
PRIMER_LEFT_3_PENALTY=0.114470
PRIMER_RIGHT_3_PENALTY=0.481423
PRIMER_LEFT_3_SEQUENCE=ATTGCTGTGAGGGAAAGAGAAG
PRIMER_RIGHT_3_SEQUENCE=GTGACCGCAATTCAAAATACAG
PRIMER_LEFT_3=891,22
PRIMER_RIGHT_3=1186,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=59.519
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.6337
PRIMER_LEFT_4_PENALTY=0.152322
PRIMER_RIGHT_4_PENALTY=0.481423
PRIMER_LEFT_4_SEQUENCE=GGAAGATATGGATGGAAGTGGA
PRIMER_RIGHT_4_SEQUENCE=GTGACCGCAATTCAAAATACAG
PRIMER_LEFT_4=977,22
PRIMER_RIGHT_4=1186,22
PRIMER_LEFT_4_TM=60.152
PRIMER_RIGHT_4_TM=59.519
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_03488 Ps_contig_mira-and-tgicl-ass_franssen_30135
SEQUENCE=TAGGAACGTAATAATAATTTNTAAAAGGGTAAGTACAGAAGAATTTGTAGGAGGCCAGCAAATACAAATTTAGTCTCTTGGTCCTCTGTGTCAGGAAAGGACGTAATCATCCTAGAAAAGGACARGGTACCACCAAGGAACAATTGATRAAAATACTGTTTGGCAACAAATTCGGGGTAGTTATCCTGAAGCAATTGAAGTGCCTGATTAGTAGCTTGTCTAAGCTCCTTCTTAACAAATCCAGGAGAATTTTTAAGATCATTAATTTGAACAATAGTAGCGATTCCAGATGGAGCAAAGTCGAGTTTTCTAACACTTTTCTCCAATGACTGAATCCTCCAACGGATAAACTTGTTTCTCTTCTCGTCATCAGAGAAACTTTTTTGATACAATTCCTTGTTCTCAAACTCGCCAAAAACATTGTAGTAAACCGGGTGACCTTCTTTGTCATAACCATCGGTGAAAACAACTTTGTCCCAATCAGTTCCAAGATCTTCTTGAAGAAGTGCTTCAATTCCAAATTCCTTTCTCCAAAGAACAGTTTGTTTGATCATCGTGAAAGCTTCCTTCACCTTGAAATCCCTTGCTCGAAGGAATTTGAGAAGAATCACATCGCTTCTTTCATCAGCTAGCAAGGGAATTCCCCATAAATCAACTTCTTCTGGTGTAGAAGGAGGAACCTCCACATCAACAGCAACAGGCAGTTCAGTAACAGGTTGTTCACCATCACCGTTAGTAACTGCAACTGATACTATGCTTTCTTGAATAGCTTCAACCGTTTTCGCACCGTCTTCTTCAGTACTCGAAGCTATTTTTTCAACAACTTCCACCACTGTCTCCTCCACCTTCTTCTCTTCAGGTTCGGTTTTAGCTTCAGCTTCGGTTTTAGGTTCCTCGGAACTTGAAGCTTTTTCTTCTTTCTTTTCAACTTCTCCTTCAACTTTTTTCTCTTCAACGACTACTTCTTCAACCTGTTTCTCTTCTGCTACTACTTCTTCCTTCTTTTCTTCGGTTTTGTTTTCTTCTTCAGGTTTTTTGTCGTCTTGTGCGGTTACTTCAGGTGCCTTAACTGGAGGAGCGGTGAACTCGCGTTTGTTAAGTGCTTCCTTAATAAGCTGTTTGAGTTCATCGAGTGCTTTTTTCTGAAGTTCAGGAAGTTCAGAAACCACGTTGGTTTCTTCCTTGAAAGAAACCGATTGAGAAATTTTGTTCTCAGCGGCGGTCTCATCAGCAGGCTTAGCGTTCTCCGTCACAGCTTCAGCCGGCTTCTCGGGATCTTCAGGAACCGGAGTAACAACAACGGCGTCCTTGTCAGTCGAGACCTCAGCAACAGCTGCGGCGGCGGCGGTGGTGGTGGTGGTTAGAACTTCTTCAGCGGGTGGTGGATTATCGACGGGAGTTTGAGAGGTAGTTGTAACGACTTCATCAGCTGGTTTTTGGGGTTCTTGAGCCATTAGAGTGAAGTTTGAGATTAATTAGGGTATTGGGATGAAAGAGAGAAAGGTGGTGGGGAGAAATGTAATAGGGAGAGAGTGAGATTGGTTTGTTAGGAGTTCCCGNGTACTCTGTCG
TARGET=1336,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03489 Ps_contig_mira-and-tgicl-ass_franssen_30135
SEQUENCE=TAGGAACGTAATAATAATTTNTAAAAGGGTAAGTACAGAAGAATTTGTAGGAGGCCAGCAAATACAAATTTAGTCTCTTGGTCCTCTGTGTCAGGAAAGGACGTAATCATCCTAGAAAAGGACARGGTACCACCAAGGAACAATTGATRAAAATACTGTTTGGCAACAAATTCGGGGTAGTTATCCTGAAGCAATTGAAGTGCCTGATTAGTAGCTTGTCTAAGCTCCTTCTTAACAAATCCAGGAGAATTTTTAAGATCATTAATTTGAACAATAGTAGCGATTCCAGATGGAGCAAAGTCGAGTTTTCTAACACTTTTCTCCAATGACTGAATCCTCCAACGGATAAACTTGTTTCTCTTCTCGTCATCAGAGAAACTTTTTTGATACAATTCCTTGTTCTCAAACTCGCCAAAAACATTGTAGTAAACCGGGTGACCTTCTTTGTCATAACCATCGGTGAAAACAACTTTGTCCCAATCAGTTCCAAGATCTTCTTGAAGAAGTGCTTCAATTCCAAATTCCTTTCTCCAAAGAACAGTTTGTTTGATCATCGTGAAAGCTTCCTTCACCTTGAAATCCCTTGCTCGAAGGAATTTGAGAAGAATCACATCGCTTCTTTCATCAGCTAGCAAGGGAATTCCCCATAAATCAACTTCTTCTGGTGTAGAAGGAGGAACCTCCACATCAACAGCAACAGGCAGTTCAGTAACAGGTTGTTCACCATCACCGTTAGTAACTGCAACTGATACTATGCTTTCTTGAATAGCTTCAACCGTTTTCGCACCGTCTTCTTCAGTACTCGAAGCTATTTTTTCAACAACTTCCACCACTGTCTCCTCCACCTTCTTCTCTTCAGGTTCGGTTTTAGCTTCAGCTTCGGTTTTAGGTTCCTCGGAACTTGAAGCTTTTTCTTCTTTCTTTTCAACTTCTCCTTCAACTTTTTTCTCTTCAACGACTACTTCTTCAACCTGTTTCTCTTCTGCTACTACTTCTTCCTTCTTTTCTTCGGTTTTGTTTTCTTCTTCAGGTTTTTTGTCGTCTTGTGCGGTTACTTCAGGTGCCTTAACTGGAGGAGCGGTGAACTCGCGTTTGTTAAGTGCTTCCTTAATAAGCTGTTTGAGTTCATCGAGTGCTTTTTTCTGAAGTTCAGGAAGTTCAGAAACCACGTTGGTTTCTTCCTTGAAAGAAACCGATTGAGAAATTTTGTTCTCAGCGGCGGTCTCATCAGCAGGCTTAGCGTTCTCCGTCACAGCTTCAGCCGGCTTCTCGGGATCTTCAGGAACCGGAGTAACAACAACGGCGTCCTTGTCAGTCGAGACCTCAGCAACAGCTGCGGCGGCGGCGGTGGTGGTGGTGGTTAGAACTTCTTCAGCGGGTGGTGGATTATCGACGGGAGTTTGAGAGGTAGTTGTAACGACTTCATCAGCTGGTTTTTGGGGTTCTTGAGCCATTAGAGTGAAGTTTGAGATTAATTAGGGTATTGGGATGAAAGAGAGAAAGGTGGTGGGGAGAAATGTAATAGGGAGAGAGTGAGATTGGTTTGTTAGGAGTTCCCGNGTACTCTGTCG
TARGET=1348,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03490 Ps_contig_mira-and-tgicl-ass_franssen_30141
SEQUENCE=TCGGGGNAATGAAATTTAGATTTTGCGATTACAACCATTGGAGCAATCTCTATAACAAAGCGCGAAACCTAATTTCCACTCAACGCTCTTCTTCCGCCACTTTCGCCGCCATGACTGACCTCAACATCCTCAAAACCAAGCTCTGCATCATCGGAAGTGGCCCCTCCGCCCACACTGCCGCAGTATACGCGGCCCGTGCGGAGCTCAAACCAGTCCTCTTCGAGGGATGGATGGCCAACGACATCGCTCCTGGTGGTCAACTCACCACCACCACCGACGTAGAGAATTTCCCTGGATTCCCTGAAGGTATCCTTGGTGGCGAACTTATGGACCGGTGCCGCCAGCAATCTGTTCGCTTCGGGACTGAGATCTTCACCGAGACAGTTTCCAAGGTCGATTTCTCAACTCGTCCTTTTAGGGTTTTTACTGACACAAGAACTGTTGAGGCTGATTCGGTTATCGTCGCTACCGGAGCTGTTGCCAAACGGTTACCGTTTACTGGTTCGGGAGAGGGTCCGGACGGTTTCTGGAACCGAGGGATCTCAGCTTGTGCCGTATGTGACGGCGCGGCTCCGATCTTTAGAAACAAGCCGTTGGCGGTGATTGGTGGCGGAGACTCGGCCATGGAGGAAGCAACGTTCCTAACAAAATACGGTTCTGAAGTTTATATAATCCATCGTAGGGATACGTTTAGGGCTTCGAAGATTATGCAGAGCAAGGTAGCGAACAATAGCAAAATTAAGGTGATATGGAACTCGTCGGTGGTTGAGGCGTATGGGGAAGGAGAGACCAAGAGATTGCTTGGGGGTTTGAAGGTGAAGAATGTGGTGACAGAAGAGGTTTCTGATTTGAAGGTTTCTGGATTGTTCTTTGCAATTGGTCATGAACCTGCGACCAAGTTCTTGGATGGTCAACTAGAACTTGATTATGATGGATACGTTGTGACAAAGCCAGGGACCACAGAGACTAGCGTTGAGGGAGTTTTTGCTGCTGGGGATGTTCAGGACAAGAAGTATAGACAAGCTATTACTGCTGCTGGAACTGGTTGCATGGCAGCTTTGGATGCAGAACATTTCCTGCAAGACATTGGTTCGCAACAGAATAAAAGTGATTGACTAGCTAAGGGATGTCCAGAAGTCGGAAGATGCTTAATTAGAAGCAATGTTTGATTTCTACTAATAAGATTGAAAACAGCTTGTTATGTATTCAGCTCGGATCTTCATTTGTTCGTTGTTATTCAAAATTTAATTTAATCACGGTGTTAAACTTGCATTTTTTGTCACATTGAATTAATCATGGAGTTAAACTTGCATTATTTGTANNAGTCTCACAGACATTACGTTTTAATTTGTTACCTAACCAAAGTTTGAA
TARGET=263,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4377
PRIMER_LEFT_PENALTY=0.420772
PRIMER_RIGHT_PENALTY=0.016950
PRIMER_LEFT_SEQUENCE=ATGACTGACCTCAACATCCTCA
PRIMER_RIGHT_SEQUENCE=GAAATCGACCTTGGAAACTGTC
PRIMER_LEFT=110,22
PRIMER_RIGHT=400,22
PRIMER_LEFT_TM=59.579
PRIMER_RIGHT_TM=59.983
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.4377
PRIMER_LEFT_1_PENALTY=0.420772
PRIMER_RIGHT_1_PENALTY=0.016950
PRIMER_LEFT_1_SEQUENCE=CATGACTGACCTCAACATCCTC
PRIMER_RIGHT_1_SEQUENCE=GAAATCGACCTTGGAAACTGTC
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=400,22
PRIMER_LEFT_1_TM=59.579
PRIMER_RIGHT_1_TM=59.983
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.5303
PRIMER_LEFT_2_PENALTY=0.420772
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=CATGACTGACCTCAACATCCTC
PRIMER_RIGHT_2_SEQUENCE=TGAGAAATCGACCTTGGAAACT
PRIMER_LEFT_2=109,22
PRIMER_RIGHT_2=403,22
PRIMER_LEFT_2_TM=59.579
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.5303
PRIMER_LEFT_3_PENALTY=0.420772
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=CATGACTGACCTCAACATCCTC
PRIMER_RIGHT_3_SEQUENCE=GAGAAATCGACCTTGGAAACTG
PRIMER_LEFT_3=109,22
PRIMER_RIGHT_3=402,22
PRIMER_LEFT_3_TM=59.579
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=0.5303
PRIMER_LEFT_4_PENALTY=0.420772
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=ATGACTGACCTCAACATCCTCA
PRIMER_RIGHT_4_SEQUENCE=TGAGAAATCGACCTTGGAAACT
PRIMER_LEFT_4=110,22
PRIMER_RIGHT_4=403,22
PRIMER_LEFT_4_TM=59.579
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_03491 Ps_contig_mira-and-tgicl-ass_franssen_30147
SEQUENCE=AGAGAAAGTAAGAAAGAAAGAAAAAAAAGTAAGCAGATAACAATGGCGATGGCTTTCAAAATGGCTACTACTGGGATGTGGGTAACTGAAGAGTGCAAGAGCTCTTTCATGGAGATGAAGTGGAAAAAGGTGCATAGATACATAGTTTTCAAGATCGATGAAAGGTCGAGGCTTGTGACCGTTGATAAGGTTGGTGGTCCTGGAGAAAACTATGATGATCTTGCTGCTTCTTTGCCTAACGATGATTGTAGATATGCTGTTTTTGATTTCGATTTTGTCACGGTTGATAACTGCCGCAAAAGCAAGATCTTCTTCATTGCATGGTCACCAACTGCATCAAGGATAAGAGCAAAGATGCTATACGCAACATCAAAGGATGGGTTGAGAAGAGCTTTAGATGGAATCTCATATGAAGTTCAAGCAACTGATCCAACTGAGATGGGATTTGATGTAATTCAAGACAGAGCCAAATAGATTCCCCATTTAATCATGTGCCAAACTAATCATCTTACTTTTGTTATAATTATAATAATGATGATAATTATAAATCAAGTAACTCCAATCCAATGACTCTATGACATTGTTCTCTTTTTATGGTATAGTTTTTCTTACTTTTAAATTAATGGTGGTAATTTTGGCCACCTATTTT
TARGET=10,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03492 Ps_contig_mira-and-tgicl-ass_franssen_30156
SEQUENCE=TTTTAAAGTTAAATTACAGAACAGAATGAATCAAATATGTCAAGGCTTAACAGGCTCATCGTCGTCATCATCCTCAATGTCCCAACTCAAGTCTTCCTCTTCCTCTGCAGTACTAAGGCGCTTCAGCAAATCAACCTTGCTGGTGCTACCACCCTGACTCTCAGATTGAGCTGCTTTCTTATCATCAATACTGCTAAGATCCTCTATATCATCCCACCCAAGATCCTCTTCCTCCTCATTTTCCATGGGCTGACTCTCAACCACTGATGACTCATTATTCTTACCCGGAGATTTTACATCAGAAGCCTTCGTTTCCAGTATTACCACTTTCTCATTGGACGCAGCCGCCGAACCATCCTTATTTGCAGCATCATCGCCCATTTCCAAATTTGATGCTTTATCAACGCCGCCATCCTCATTCTTTTCCTCACTTCCACCACCCATCTCATGAACAACTTCAGCTTTCTCAACAACAGGTGATTCTTCAACAGACTTAACCATCTCATTACCATGTTCATTGCTTTGCATCGAATTGTCTCTCTTTTCTACTTCCACACTCAATCTCTTCTCATCACTAGCACCAGAGCTAGATGCACCAATTTTCAAATCAGTATCTACACTTTTCCCCAAATTTTCACCACCAATTTCCTTATTGATCACAATCTCTGGTTTAACTTCACCTTCATTCTCCTCCTCATCATCTTCAACATCCCAACTCAAATCCTCCTCTTCCCTGGATATTCTTCTAACAAGCCTAGCCCTAACATCTTCAGCTTTCTTGAGTCTATAAACCTTATAATAATACCTATACCAGAAAGTTTCATGATCAACAGCATTTGGAACAACCTTTTTATAAACACTCTCCATTGCATCATTCTCTCTAAAAAGACTCTCCATTTCATCACTTCTCCCATCCAGTGCAAACCCTAATTTCCACTCATTGAATTCGTTCAAATCCTCAGGCACCTCAGTGTAAGTGCTAACATCCCCCTGAATAGCACCAACCTGGGCATCAAACCTACTATATCGCTTCGAATCCAATCGGCTCGAATCGCGACTCGCCGTGGTGGTACCATTATCAGAATCTGAATCGAGGTTAACGGCAAGGATGGCTTCCTTACCTTGAGATATGATCTGAGCAGTTGATTTAATGACGGTGCTACCGATCTCACCAACCGAATCTTGAGCAACTTCGATCTCGCTCTTCAAGCCGGTGCTGAATTCCTGTAGATCACGACGATAAGTCTCGATTATGGATTCGGATTTGGATGTGAGGGTTTTGATAAGTCCACCAAAGTTCCAAGCGTCGTCGGCGCCGGAATTGGGATTGGGATCTGAATCGTTGGGTTCGGGTTGAGGAGGATCTGGATCATCGGAGAAAACGGATTTGAAGAAATTCATGAGAAAAAGGTTAACGAAAGATCACGAAGTGAACGAAGTTATCTGAAGCCTGCAGGAGAGGGGCTTGGTGTCTACAGCAATGAAGTATTCCATTTTCAGATTTGAAGATGATATAAGAAAAATTACAGGAGAGGTTTTGTTTTGTTTTTCTTACAGCCCACTACAGCAGTGTTTTTGTTTTTACAACAATTCTCT
TARGET=1535,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0858
PRIMER_LEFT_PENALTY=0.080887
PRIMER_RIGHT_PENALTY=0.004903
PRIMER_LEFT_SEQUENCE=AACGAAGTTATCTGAAGCCTGC
PRIMER_RIGHT_SEQUENCE=CACTGCTGTAGTGGGCTGTAAG
PRIMER_LEFT=1432,22
PRIMER_RIGHT=1571,22
PRIMER_LEFT_TM=59.919
PRIMER_RIGHT_TM=59.995
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=140
PRIMER_PAIR_PENALTY_1=0.0936
PRIMER_LEFT_1_PENALTY=0.088662
PRIMER_RIGHT_1_PENALTY=0.004903
PRIMER_LEFT_1_SEQUENCE=GGGTTTTGATAAGTCCACCAAA
PRIMER_RIGHT_1_SEQUENCE=CACTGCTGTAGTGGGCTGTAAG
PRIMER_LEFT_1=1274,22
PRIMER_RIGHT_1=1571,22
PRIMER_LEFT_1_TM=60.089
PRIMER_RIGHT_1_TM=59.995
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.0953
PRIMER_LEFT_2_PENALTY=0.090376
PRIMER_RIGHT_2_PENALTY=0.004903
PRIMER_LEFT_2_SEQUENCE=GATCTGGATCATCGGAGAAAAC
PRIMER_RIGHT_2_SEQUENCE=CACTGCTGTAGTGGGCTGTAAG
PRIMER_LEFT_2=1361,22
PRIMER_RIGHT_2=1571,22
PRIMER_LEFT_2_TM=59.910
PRIMER_RIGHT_2_TM=59.995
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.2169
PRIMER_LEFT_3_PENALTY=0.080887
PRIMER_RIGHT_3_PENALTY=0.135980
PRIMER_LEFT_3_SEQUENCE=AACGAAGTTATCTGAAGCCTGC
PRIMER_RIGHT_3_SEQUENCE=ACACTGCTGTAGTGGGCTGTAA
PRIMER_LEFT_3=1432,22
PRIMER_RIGHT_3=1572,22
PRIMER_LEFT_3_TM=59.919
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=141
PRIMER_PAIR_PENALTY_4=0.2169
PRIMER_LEFT_4_PENALTY=0.080887
PRIMER_RIGHT_4_PENALTY=0.135980
PRIMER_LEFT_4_SEQUENCE=AACGAAGTTATCTGAAGCCTGC
PRIMER_RIGHT_4_SEQUENCE=AACACTGCTGTAGTGGGCTGTA
PRIMER_LEFT_4=1432,22
PRIMER_RIGHT_4=1573,22
PRIMER_LEFT_4_TM=59.919
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=142
=
PRIMER_SEQUENCE_ID=SSR_03493 Ps_contig_mira-and-tgicl-ass_franssen_30157
SEQUENCE=AGCAGTATTGATGATAAGAAAGCAGCTCAATCTGAGAGTCAGGGTGGTAGCACCAGCAAGGTTGATTTGCTGAAGCGCCTTAGTACTGCAGAGGAAGAGGAAGACTTGAGTTGGGACATTGAGGATGACGACGACGAGCCTGTTAAACCTTGACATATTTGATTCATTCTGTTCTGATTTCTTTAAAAAAATATATGTGCATTTTTATCAAAAAGAAATTTGCATGTTTTTATTTATTTATTTGTTGAACTAAGATAGAAGTAATGTTGTGAATTTGTGATATGTATTTCTATACCTGAAATGCATAGAAAAGCATTCTTTTTCTGTAGTATTGTTGAGCGGTATCTTTTATTTGATTTATACCTTAGTTGTTGTGTAATTATAAGTTCCCTCCAATTCCATTGATCACCTTGAAA
TARGET=229,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3485
PRIMER_LEFT_PENALTY=0.171757
PRIMER_RIGHT_PENALTY=0.176730
PRIMER_LEFT_SEQUENCE=GACGAGCCTGTTAAACCTTGAC
PRIMER_RIGHT_SEQUENCE=CAATGGAATTGGAGGGAACTTA
PRIMER_LEFT=132,22
PRIMER_RIGHT=403,22
PRIMER_LEFT_TM=60.172
PRIMER_RIGHT_TM=60.177
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.3587
PRIMER_LEFT_1_PENALTY=0.171757
PRIMER_RIGHT_1_PENALTY=0.186949
PRIMER_LEFT_1_SEQUENCE=GACGAGCCTGTTAAACCTTGAC
PRIMER_RIGHT_1_SEQUENCE=AAGGTGATCAATGGAATTGGAG
PRIMER_LEFT_1=132,22
PRIMER_RIGHT_1=411,22
PRIMER_LEFT_1_TM=60.172
PRIMER_RIGHT_1_TM=60.187
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.5381
PRIMER_LEFT_2_PENALTY=0.361394
PRIMER_RIGHT_2_PENALTY=0.176730
PRIMER_LEFT_2_SEQUENCE=CTTGAGTTGGGACATTGAGGAT
PRIMER_RIGHT_2_SEQUENCE=CAATGGAATTGGAGGGAACTTA
PRIMER_LEFT_2=104,22
PRIMER_RIGHT_2=403,22
PRIMER_LEFT_2_TM=60.361
PRIMER_RIGHT_2_TM=60.177
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.7101
PRIMER_LEFT_3_PENALTY=0.533373
PRIMER_RIGHT_3_PENALTY=0.176730
PRIMER_LEFT_3_SEQUENCE=CGAGCCTGTTAAACCTTGACAT
PRIMER_RIGHT_3_SEQUENCE=CAATGGAATTGGAGGGAACTTA
PRIMER_LEFT_3=134,22
PRIMER_RIGHT_3=403,22
PRIMER_LEFT_3_TM=60.533
PRIMER_RIGHT_3_TM=60.177
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.7203
PRIMER_LEFT_4_PENALTY=0.533373
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=CGAGCCTGTTAAACCTTGACAT
PRIMER_RIGHT_4_SEQUENCE=AAGGTGATCAATGGAATTGGAG
PRIMER_LEFT_4=134,22
PRIMER_RIGHT_4=411,22
PRIMER_LEFT_4_TM=60.533
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_03494 Ps_contig_mira-and-tgicl-ass_franssen_30159
SEQUENCE=ATGGAATGTAATCCTTTGATATACTTTGGCAGTTGTTGTGGATAAGAAAAACACAGATAGCAGTTTTCGTAGCGACTGAACTTAGCCGGCGGATATGGAAGCAGTACTAGCATCCCGCCATTTGTCGACACCGTTCTCTCACAATCGAACCACCGCCTCCTCCTCCTCCTCCTCCTCTTCTATCAAACCTTCCTCTTCCATTTCTTTTCGGAATTCCTTCGCGAGAGAGATATGGGGTTTGGTTCAGTCCAAGAGCTTAACTTCCAGAAGAACGGACATGATTCGTCCGGTTGTTGTCAGGGCTGAAATGTTCGGACAGTTAACATCTGGTCTTGAATCTGCCTGGAACAAGCTCAAACGAGAAGAGGTTTTGACTAAAGAGAATATTGTGGAGCCTATGCGAGATATTCGGAGAGCTCTTTTAGAAGCTGATGTTAGTCTTCCTGTTGTAAGAAGGTTTGTGCAATCTGTTACTGATAAAGCTGTGGGTGTTGGAGTCACTCGAGGAGTCAAACCCGATCAACAATTGGTTAAGATTGTTCATGATGAACTTGTACAATTGATGGGTGGAGAGGTCTCTGAACTAACTTTTGCCAATTCGGGGCCCACTGTCATTTTATTGGCTGGACTCCAGGGAGTTGGGAAGACAACTGTATGTGCAAAGTTAGCAACTTATTTCAAGAAACAGGGAAAGAGTTGTATGTTAGTTGCTGGGGATGTTTACAGACCTGCTGCTATTGACCAGCTGACTATTTTGGGCAAGCAGGTGGATGTGCCTGTCTATGCAGCAGGTACCGATGTTAAGCCTTCAGTGATTGCTAAACAAGGTTTTGAAGAGGCCAAAAAGAAGAAAATTGACGTGGTTATTGTTGACACTGCTGGTAGGCTGCAGATAGACAAAGCTATGATGGACGAGTTAAAAGAAGTGAAACAGGTACTGAATCCAACAGAAGTTTTGCTAGTTGTGGATGCTATGACAGGGCAAGAAGCAGCAGCTCTGGTGACTACTTTCAATCTTGAGATTGGAATAACTGGTGCCATCTTGACAAAGTTGGATGGTGATTCAAGAGGTGGAGCAGCTTTGAGTGTCAAAGAGGTATCTGGAAAACCAATTAAGCTGGTAGGACGGGGAGAACGCATGGAAGATCTTGAACCTTTTTACCCCGACCGAATGGCAGGACGTATACTAGGAATGGGAGATGTTCTTTCATTTGTGGAAAAGGCACAAGAAGTTATGCTTGAAGAAGATGCTGAAGACATGCAAAAGAAAATCATGAGTGCAAAGTTTGACTTTAATGATCTTCTAAAGCAAACTCGTACTGTTGCTAAAATGGGTTCTGTGTCTCGAGTCCTTGGGATGATTCCTGGTATGGCAAAGGTTACTCCTGCTCAAATTCGAGAAGCAGAGAAGAATTTGCAGTTTATGGAAGTGATAATAGAAGCAATGACCCCTGAGGAAAGGGGAAAGCCAGAACTGTTAGCAGAATCCCCTGTCAGGAGGAAAAGAGTTGCTCAGGAATCAGGGAAGACAGAGCAGCAGGTAAGCCAACTCGTAGCTCAACTATTTCAAATGCGTGTTCGGATGAAGAAGCTAATGGGTGTAATGGAAGGTGGATCAATGCCAACACTTAGTAATCTTGAGGAAGCACTTAAAGCAGACGAAAAGGCTCCACCTGGCACTGCTAGGAGGAGAAAGAAAGGAGGTTTAAGAAGGCTATTTAAAGGCCGATCTTCGAAGAGTTCGTTGCCAGCTCCTCGCGGATTTGGGAGCAAAAACTGAAAATTTTCTAAAGATGATTGACACTCAATAAATATGGTAGGAGAGTTTGTTATTTGGATCAACAAGTCGCAAGCAATCAACTGGTTTGATTTAGCTTTCTTAAGCTAAAATGATTGCCTTCCATTATTAGATCAATTCATATAGTAGCGAGTGTTGTTTCGCTGGTGAAATTTAGGCAGAAATGGAATGTAAGCGCGGGATGTGCAAATATAAGAAGTATTTGATTTTCTAACTTGCAAGCTTGACTTTGTCATCTCATTTGAATTTTTGAAATGCATATAATGGATGGCCAAAATCAAATGCATGTAATTCGAAAAAAGATGGTTTAAGAATCTTCAATGGAGATTTCCCGCAATCTAGACCAGGTCCTTGCACTCTGTTTGTTTTGTAATAAATTTAACTCTTCTGGGTATTTGAATGAATAGATAGCAATGTTTTGTGTCGTAAAACT
TARGET=156,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1401
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.107763
PRIMER_LEFT_SEQUENCE=TTTGGCAGTTGTTGTGGATAAG
PRIMER_RIGHT_SEQUENCE=TCCGTTCTTCTGGAAGTTAAGC
PRIMER_LEFT=24,22
PRIMER_RIGHT=275,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.1401
PRIMER_LEFT_1_PENALTY=0.032347
PRIMER_RIGHT_1_PENALTY=0.107763
PRIMER_LEFT_1_SEQUENCE=CTTTGGCAGTTGTTGTGGATAA
PRIMER_RIGHT_1_SEQUENCE=TCCGTTCTTCTGGAAGTTAAGC
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=275,22
PRIMER_LEFT_1_TM=60.032
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.2193
PRIMER_LEFT_2_PENALTY=0.032347
PRIMER_RIGHT_2_PENALTY=0.186949
PRIMER_LEFT_2_SEQUENCE=TTTGGCAGTTGTTGTGGATAAG
PRIMER_RIGHT_2_SEQUENCE=TGAACCAAACCCCATATCTCTC
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=245,22
PRIMER_LEFT_2_TM=60.032
PRIMER_RIGHT_2_TM=60.187
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.2193
PRIMER_LEFT_3_PENALTY=0.032347
PRIMER_RIGHT_3_PENALTY=0.186949
PRIMER_LEFT_3_SEQUENCE=CTTTGGCAGTTGTTGTGGATAA
PRIMER_RIGHT_3_SEQUENCE=TGAACCAAACCCCATATCTCTC
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=245,22
PRIMER_LEFT_3_TM=60.032
PRIMER_RIGHT_3_TM=60.187
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.4611
PRIMER_LEFT_4_PENALTY=0.032347
PRIMER_RIGHT_4_PENALTY=0.428727
PRIMER_LEFT_4_SEQUENCE=TTTGGCAGTTGTTGTGGATAAG
PRIMER_RIGHT_4_SEQUENCE=GAAATGGAAGAGGAAGGTTTGA
PRIMER_LEFT_4=24,22
PRIMER_RIGHT_4=203,22
PRIMER_LEFT_4_TM=60.032
PRIMER_RIGHT_4_TM=59.571
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=180
=
PRIMER_SEQUENCE_ID=SSR_03495 Ps_contig_mira-and-tgicl-ass_franssen_30161
SEQUENCE=ACCTTGCATAGTTTTGGTAGCCAAACATGGGATGCTAGTTTGATCATTCAAGCTTTGCTTGCCACTAACCTAATTGAGGATGTTGGTCCAATACTTACAAAAGCACATGAATTCATCAAGAAGTCTCAGGTTAGGGACAACCCTTCAGGAGACTTTAAGAGCATGTATCGTCATATCTCTAAAGGGTCATGGACCTTCTCTGATAAAGATCATGGATGGCAAGTTTCTGATTGCACAGCAGAAAGTTTAAAGTGTTGTCTGCTTTTGTCTATGTTGCCTCCGGAGATTGTGGGGGAAAAGATGGAACCTGAAATGTTATATGATTCAGTCAATATTCTATTATCACTTCAGGGTAAAAAAGGTGGTTTACCAGCTTGGGAGCCATCAGAAGCTGTAGAATGGTTGGAACTATTCAATCCAATAGAATTTCTTGAGGAAATTGTAGTTGAGCGTGAATATGTTGAATGCACTTCATCAGNAATYCAAGCTTTAGTTTTAGTTTAAAAAACTATATCCAGAACACAGAAAGAAAGAGGTTAGAAAATTTCATTGACAAAATGCAGTTACGATTTCTCGAATATAAACAAACAAGTGATGGTTCATGGTATGGAAATTGGGGAATTTGCTTCACTTATGGTTCTTGGTTTGCACTTAACGGTCTAGTGGCTGCTGGCAAGACTTATGATAATTGTGCAGCCATTCGCAAAGGTGTTGAATTTTTGCTTACAACACAAAGAGAGGATGGTGGGTGGGGGGAGAGCCATCTTTCTAGCTCAAAAAAGATATATGTACCTCTTGAAAGAAGTCAATCAAATATTGTGCAGACATCATGGGCTATTATGGGTTTAATTCATGCTGGTCAGATGGAGAGAGACCCTACTCCTCTTCATCGTGCTGTAAAGTTGATCATCAATTTCCAACAAGAAGAGGGTGATTGGCCCCAACAGGAACTTACAGGAGTATTCATGAAAAATTGTATGTTACAATATGCAATGTATAGAGATATTTTCCCAACATGGGCTCTAGCTGAATATCGTAGACGGATTCTGTTGGCTTCACCAGCAGTTGCAATTTAAAATGAAACATTTATGCAATTATGTGTGTATCTTACTTCATAAAAGATATTTGAAAGATATATATATATATATACACAAATTAGTCTCGGTATAAGAT
TARGET=1133,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03496 Ps_contig_mira-and-tgicl-ass_franssen_30162
SEQUENCE=AAAAGAGCTAAAATTTGTTTGAAATAATTATCATTTTGTTGATAAATATTAATAATAATAATAATATGTGATAGTNGGRNATATGAGAAAAATTTTGCATTTGAGTAACAGAATCCGGTAGTTCTTACCGAAAACAAGAGGCCAGCGTCTCGTGTGTCTGGTTTTCTCTATCCCAAACCCCAACAACAACAAAGGGGGAGAATCATGGCAGAGTCGTTTCAAGTGGCCACTTCTTCTCTATGTCATCTCAACGGTTCTCACCGGACGCCAATTCCTCTTTCTCGCTTCATGGCTTCAGGACCTGGCTTTCGCTCTCATCACTCCCTCTATTTCACTCGCTCTTCCTCTTCGCACTTCTTCGGGACTAATGCTAATTCCATTTCTTTCGCTTCACCTCGAGTTGCCCGAACAAGGAGAAGTTTTTCGGTTTTCGCCATGGCCACTGAGGATGGCAAGCGTGTTGTCCCGTTGAAGGATTATCGCAACATTGGCATCATGGCTCACATAGACGCTGGAAAGACAACTACAACGGAAAGAATTCTGTTCTATACCGGAAGAAACTACAAAATTGGAGAGGTGCACGAGGGGACAGCCACAATGGACTGGATGGAACAGGAACAAGAAAGAGGGATTACAATTACTTCTGCTGCAACCACCACGTTTTGGGATAAGCACAGGATCAATATCATTGATACTCCGGGTCATGTTGACTTCACCTTAGAAGTGGAGCGTGCTCTTCGGGTGTTGGATGGAGCTATATGCTTGTTTGACAGTGTTGCTGGTGTGGAACCACAGTCAGAAACTGTGTGGAGGCAAGCGGATAGATACGGGGTCCCTCGGATTTGTTTTGTCAATAAAATGGACCGACTTGGAGCGAATTTTTTCCGAACAAGAGACATGATAGTGACGAATTTGGGTGCCAAACCACTTGTACTTCAACTACCGGTTGGGGCAGAGGATACCTTTAAGGGAGTTATTGATATTGTTAGGATGAAAGCTATAGTTTGGGGTGGAGAAGAGCTTGGTGCAAAGTTTTCTTATGAAGATATACCGGAAGATCTCCTAGAGCAGGCTCAGGATTACAGATCACAGATGATAGAAACCATAGTTGAGTTAGATGACGAGGTCATGGAGAACTACCTAGAAGGAGTTGAACCAGACGAAAAAACTATAAAAAGATTAATTAGAAAGGGGTCCATAGCAGCCACTTTTGTGCCAGTGTTGTGTGGGTCAGCTTTCAAAAACAAGGGAGTCCAACCGTTGCTAGATGCCGTAGTGGACTATCTACCATCACCACTTGATGTACCACCTATGAAGGGCACTGACCCTGAAAACCCGGAAACAATAATAGAAAGGATTGCAAGTGATGATGAATCATTTTCTGGACTGGCTTTTAAGATCATGAGTGATTCATTTGTAGGCTCCCTTACGTTCGTCAGGGTGTATTCTGGAAAGCTAGCCGCAGGGTCTTATGTACTCAATTCAAACAAAGGGAAAAGGGAAAGAATTGGTAGACTTCTAGAAATGCATGCTAACAGTAGAGAAGACGTTAGAGTAGCTTTAACAGGAGATATTGTTGCTCTTGCTGGTTTAAAAGACACAATAACAGGTGAAACGTTGTGTGACCCTGAGAGTCCGGTCGTGCTTGAGCGGATGGACTTCCCTGATCCTGTGATTAAGATTGCAATTGAACCCAAAAACTAAAGCTGACATTGACAAGATGGCAGCTGGTTTAGTCAAGCTTGCACAGGAAGACCCTTCTTTCCACTTCTCCCGTGATGAAGAGATAAATCAGACTGTCATTGAAGGAATGGGAGAATTGCATCTGGAAATCATTGTTGATCGCCTTAAAAGAGAATATAAGGTAGAAGCTAATGTTGGAGCTCCCCAAGTAAACTACAGGGAAAGCATTTCCAAAATCTCACAAGTGAGGTATGTGCACAAGAAACAATCAGGCGGACAAGGTCAGTTTGCAGATATAACCGTACGGTTTGAACCCATGGACCCAGGGAGTGGATATGAATTCAGAGTGAAATCAAAGGAGGTGTAGTTCCGAAAGAATACGTTCCTGGAGTGGTTAAAGGATTGGAAGAGTGTATGAGCAATGGTGTTCTAGCCGGCTTTCCGGTTGTTGATGTACGCGCAGTACTTGTGGATGGTACTTACCATGATGTGGATTCAAGTGTGCTGGCTTTCCAGTTGGCAGCAAGAGGAGCTTTTCGGGAAGGAATAAGAAAAGCCGGACCGAGAATGCTCGAACCTGTAATGAAAGTTGAAGTTGTTACTCCTGAAGAACATTTAGGAGATGTAATTGGTGATCTCAACTCAAGAAGAGGTCAGATCAACAGTTTTGGTGACAAACCAGGTGGACTTAAGGTGGTTGATTCACTAGTACCTCTTGCTGAGATGTTTCAGTATGTCAGTACACTTAGAGGGATGACAAAGGGTCGTGCATCCTATACAATGCAATTAGCCATGTTTGATGTGGTACCTCAACACATTCAGAATCAACTTGCAACAAAAGTGCAAGAAGTTGCTGCTTGATTTTGCTGTATTATCTTCCCTTTGATACCTGTATTATGTTCATTGTCTTATAGTTGTATTCTTTGGGGGTATTTTTTATTGTAATTTTTCATGTACTTATACAAGGTTAGCCGAAAAAGAACATTCAGAAGGAAAAAGAAAACGACACGATTTTCTCTTTCATGAACTTTTTCAGGGCAAATTTTGCTGTATTTTTGTTTTGTTTTTTGTTTGGAGAATGTTAGGTCTGAAAACTTGGATTTTGCAGAATCATCTAATTCTCAAAAACACATTTATTTACCCTGTTTYAA
TARGET=50,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03497 Ps_contig_mira-and-tgicl-ass_franssen_30162
SEQUENCE=AAAAGAGCTAAAATTTGTTTGAAATAATTATCATTTTGTTGATAAATATTAATAATAATAATAATATGTGATAGTNGGRNATATGAGAAAAATTTTGCATTTGAGTAACAGAATCCGGTAGTTCTTACCGAAAACAAGAGGCCAGCGTCTCGTGTGTCTGGTTTTCTCTATCCCAAACCCCAACAACAACAAAGGGGGAGAATCATGGCAGAGTCGTTTCAAGTGGCCACTTCTTCTCTATGTCATCTCAACGGTTCTCACCGGACGCCAATTCCTCTTTCTCGCTTCATGGCTTCAGGACCTGGCTTTCGCTCTCATCACTCCCTCTATTTCACTCGCTCTTCCTCTTCGCACTTCTTCGGGACTAATGCTAATTCCATTTCTTTCGCTTCACCTCGAGTTGCCCGAACAAGGAGAAGTTTTTCGGTTTTCGCCATGGCCACTGAGGATGGCAAGCGTGTTGTCCCGTTGAAGGATTATCGCAACATTGGCATCATGGCTCACATAGACGCTGGAAAGACAACTACAACGGAAAGAATTCTGTTCTATACCGGAAGAAACTACAAAATTGGAGAGGTGCACGAGGGGACAGCCACAATGGACTGGATGGAACAGGAACAAGAAAGAGGGATTACAATTACTTCTGCTGCAACCACCACGTTTTGGGATAAGCACAGGATCAATATCATTGATACTCCGGGTCATGTTGACTTCACCTTAGAAGTGGAGCGTGCTCTTCGGGTGTTGGATGGAGCTATATGCTTGTTTGACAGTGTTGCTGGTGTGGAACCACAGTCAGAAACTGTGTGGAGGCAAGCGGATAGATACGGGGTCCCTCGGATTTGTTTTGTCAATAAAATGGACCGACTTGGAGCGAATTTTTTCCGAACAAGAGACATGATAGTGACGAATTTGGGTGCCAAACCACTTGTACTTCAACTACCGGTTGGGGCAGAGGATACCTTTAAGGGAGTTATTGATATTGTTAGGATGAAAGCTATAGTTTGGGGTGGAGAAGAGCTTGGTGCAAAGTTTTCTTATGAAGATATACCGGAAGATCTCCTAGAGCAGGCTCAGGATTACAGATCACAGATGATAGAAACCATAGTTGAGTTAGATGACGAGGTCATGGAGAACTACCTAGAAGGAGTTGAACCAGACGAAAAAACTATAAAAAGATTAATTAGAAAGGGGTCCATAGCAGCCACTTTTGTGCCAGTGTTGTGTGGGTCAGCTTTCAAAAACAAGGGAGTCCAACCGTTGCTAGATGCCGTAGTGGACTATCTACCATCACCACTTGATGTACCACCTATGAAGGGCACTGACCCTGAAAACCCGGAAACAATAATAGAAAGGATTGCAAGTGATGATGAATCATTTTCTGGACTGGCTTTTAAGATCATGAGTGATTCATTTGTAGGCTCCCTTACGTTCGTCAGGGTGTATTCTGGAAAGCTAGCCGCAGGGTCTTATGTACTCAATTCAAACAAAGGGAAAAGGGAAAGAATTGGTAGACTTCTAGAAATGCATGCTAACAGTAGAGAAGACGTTAGAGTAGCTTTAACAGGAGATATTGTTGCTCTTGCTGGTTTAAAAGACACAATAACAGGTGAAACGTTGTGTGACCCTGAGAGTCCGGTCGTGCTTGAGCGGATGGACTTCCCTGATCCTGTGATTAAGATTGCAATTGAACCCAAAAACTAAAGCTGACATTGACAAGATGGCAGCTGGTTTAGTCAAGCTTGCACAGGAAGACCCTTCTTTCCACTTCTCCCGTGATGAAGAGATAAATCAGACTGTCATTGAAGGAATGGGAGAATTGCATCTGGAAATCATTGTTGATCGCCTTAAAAGAGAATATAAGGTAGAAGCTAATGTTGGAGCTCCCCAAGTAAACTACAGGGAAAGCATTTCCAAAATCTCACAAGTGAGGTATGTGCACAAGAAACAATCAGGCGGACAAGGTCAGTTTGCAGATATAACCGTACGGTTTGAACCCATGGACCCAGGGAGTGGATATGAATTCAGAGTGAAATCAAAGGAGGTGTAGTTCCGAAAGAATACGTTCCTGGAGTGGTTAAAGGATTGGAAGAGTGTATGAGCAATGGTGTTCTAGCCGGCTTTCCGGTTGTTGATGTACGCGCAGTACTTGTGGATGGTACTTACCATGATGTGGATTCAAGTGTGCTGGCTTTCCAGTTGGCAGCAAGAGGAGCTTTTCGGGAAGGAATAAGAAAAGCCGGACCGAGAATGCTCGAACCTGTAATGAAAGTTGAAGTTGTTACTCCTGAAGAACATTTAGGAGATGTAATTGGTGATCTCAACTCAAGAAGAGGTCAGATCAACAGTTTTGGTGACAAACCAGGTGGACTTAAGGTGGTTGATTCACTAGTACCTCTTGCTGAGATGTTTCAGTATGTCAGTACACTTAGAGGGATGACAAAGGGTCGTGCATCCTATACAATGCAATTAGCCATGTTTGATGTGGTACCTCAACACATTCAGAATCAACTTGCAACAAAAGTGCAAGAAGTTGCTGCTTGATTTTGCTGTATTATCTTCCCTTTGATACCTGTATTATGTTCATTGTCTTATAGTTGTATTCTTTGGGGGTATTTTTTATTGTAATTTTTCATGTACTTATACAAGGTTAGCCGAAAAAGAACATTCAGAAGGAAAAAGAAAACGACACGATTTTCTCTTTCATGAACTTTTTCAGGGCAAATTTTGCTGTATTTTTGTTTTGTTTTTTGTTTGGAGAATGTTAGGTCTGAAAACTTGGATTTTGCAGAATCATCTAATTCTCAAAAACACATTTATTTACCCTGTTTYAA
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03498 Ps_contig_mira-and-tgicl-ass_franssen_30166
SEQUENCE=GGTTTNNAACTAACAATTATAATCCAATACTTCCAACTTATAGAAATCGTACACATATATATTCTATAAACCTAAGCGTAAATATGGCATCTTCCCACCAAACATATTAGTAAGGCAGGCCACACAATCATTTTCTCTTCAAAACCCTTCTGCAAAAAATCCGGATTCCAAAGGCAAAATAAATGAATTGAAACTTAAGTAAATAACCGTCCAAAAGATAGGGCTCCGCAGCAAACATCGCAGCAGCCACTAACTGGTCAACCCACCAAAGCCTTTTCCAGCCTAAATTCTGGCAATTCATAATCATATAAGCTCATACAAAATGTAGTAGACACTAGTAGTCTCAACACGAACTGAGACTAAATCAAGAACAAAATTATGCAGAACTGGCATTCTCTGGCTTATCGGTCTCCATTGGTTCAGTAGATGCTGATGAGGCCTGACTACCATCCCCAGCATTCTCACTAGTGCTGTTGGCATTAGCATCTGCCTGGGGTTGCTGCTGCTCAGAGCCTGAAGAAGCGGGGCTTGGTGGTGCAGGAGGAGTAGCTGGCTTGGCTGGCTTTGGTTTCGTCATGATTGGCTTGCAGAACCTATCAACAGCCTCAGCTTTCTTTCTTATTTCAGCTGACAAGAGTACAGGGTTGGCATGTTTTGGAAGTGAATCCTGCTGCTGTTTCTTCTCCCTGAGCCAATTCTCAGCTTCAACGCATTCATTTAAAACCTTCTGTTTCTCATTGATGTCAATATGATCAAATTTGGGATCAGGTGACATTGCAGCTTCTCTGTAACTAGTTATACAATAGTTTAACTGATCAACTATTGTACCCCTCTCTGTGTATTCTTTGTAACGCTCCTCAATCGGGTCACCTTGCTTTTTGAGCTCTTCGAGTTTTGCAACATATACACCTTTAGTTTCATCTTCACCCTCCTCGTATAGCCAATCTTCTACCTCCTGGAGTTTAGAGGTAAAACCTTCCCTCTCTGAAGCAATGACAAAGTCTTGGTATTTGTCATTAAGCTTGTTTCTCATGTCATACACATATGCCTCTACAGCATTTTTCCTGTCCTTTGTTTCTTCCATCACACGATCTTGCAAAGCCATTTCATACTCCTTCTCTACAGCTTTCTGGACTTCCACATGTGTCATTGCTCCATAAACTAACTCTGCAACCGGAACACTTGTTTTCTTAACCTTTTTCTTTGGAGCCTTGGTATCATCAACATCCATTTGTGCAGGCTTATTTCCAGTCTCAGGGACGCCATTTTCAACCCCAGGGGTATCAGCACTAGGCTTTGCATCTTGCATATTAACATCAGCATCATTGTCATTGGAGGTGGGAGGTGCAGCAGCAGCATCAGTATCCACCTTAGTAGTTTCTTTTGCCGATTCTTTGGAATCTGAAACCTCAACTTCTTCCTCTTCCAGGAGTGTTGCCAAGTCAACAGATGCAATTCCATGAAGATTCAAACGAACTTTCACTTTAACTTTTGCCTTCTCGCTATGTTTAGCTTCAAAAGGACCAATGGTATATGTGCTGATCCTAGCAGGTGTTTGCAGCCCACCCGCATCACTATATTGTGCATCAATAGAAAATGTTCCCGACCTGTAGAATGTCAGTGCCTTGATACTTGGTATGGGATTTCCCTTGGGAAAAACAAGGGAACTCTGCTGATTATTGGTTCCACTATCATGTGCATCTGGACCAGACCCTTTCCACGATAGTGAGATTGAGAAAGGAAAGCTTTCGTTGACCTGAAATTCTCGCACTTTAAAAGTTGGACTAAGAATAGCACATTGCAATGCAGCCCCTTTGGCAACGCATTCACTGGCGTTCATTGTCCTCCTGGGCTCCTTCTTAGAAAAAACTCAGTCAATATTTTGTTTATAGCTGGAACA
TARGET=1347,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0341
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=TTCTTTGGAGCCTTGGTATCAT
PRIMER_RIGHT_SEQUENCE=CATGGAATTGCATCTGTTGACT
PRIMER_LEFT=1199,22
PRIMER_RIGHT=1461,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.0380
PRIMER_LEFT_1_PENALTY=0.037936
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=ATCAGCATCATTGTCATTGGAG
PRIMER_RIGHT_1_SEQUENCE=CATGGAATTGCATCTGTTGACT
PRIMER_LEFT_1=1315,22
PRIMER_RIGHT_1=1461,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.0387
PRIMER_LEFT_2_PENALTY=0.037936
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=ATCAGCATCATTGTCATTGGAG
PRIMER_RIGHT_2_SEQUENCE=ACCATTGGTCCTTTTGAAGCTA
PRIMER_LEFT_2=1315,22
PRIMER_RIGHT_2=1530,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=216
PRIMER_PAIR_PENALTY_3=0.1193
PRIMER_LEFT_3_PENALTY=0.037936
PRIMER_RIGHT_3_PENALTY=0.081412
PRIMER_LEFT_3_SEQUENCE=ATCAGCATCATTGTCATTGGAG
PRIMER_RIGHT_3_SEQUENCE=AAACATAGCGAGAAGGCAAAAG
PRIMER_LEFT_3=1315,22
PRIMER_RIGHT_3=1509,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=59.919
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=0.1233
PRIMER_LEFT_4_PENALTY=0.123278
PRIMER_RIGHT_4_PENALTY=0.000037
PRIMER_LEFT_4_SEQUENCE=CTTATTTCCAGTCTCAGGGACG
PRIMER_RIGHT_4_SEQUENCE=CATGGAATTGCATCTGTTGACT
PRIMER_LEFT_4=1240,22
PRIMER_RIGHT_4=1461,22
PRIMER_LEFT_4_TM=60.123
PRIMER_RIGHT_4_TM=60.000
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_03499 Ps_contig_mira-and-tgicl-ass_franssen_30172
SEQUENCE=GTAATAAAACTAGAGAAAATTAGTTNATAAAAAAGTGCACATCACTCTTCAAATTGCATTAGATGAAAACCATACTATTGGTGAAGGGGGCCATTATGAATGGAAAGGCCAATCTAAAACTGGTTTTAATTTCAATCCCTGCGGCAATAAACCGCTAGCGGAATTAATTATTATTTACCTAATAGTATACCTGCTCTATAAATAAATAAAACAAAGCCACCGCCTATGCCGGCAACATTACAAACAATGGTTCTGAATTTGCAAAATTTGAATTGGTTCAAAATGGAAAGGAAAAACCAGCAACCTGCTGGAAATGTATCACATGTTTGATTACTGGGAGGCAAATTTGATTCCCTTTTCAATAGAAGATTTTAGTTCAGACTTGAGTTTTTCGAGACCTTCTTGCTCGAAATCTGAGAGAGAACCTAAGCCCAGAATTTCCTCAACACCATTCTTCCCAATCCTCACCTTGGAAGCAAAGAAAGGAAGTTCACTGATGATATTGGATTGCACATATGAGCACTCAACAACATCTGGAACTCCATTAAGACCTTTGAGGCAAGCATCAGCAAATATGGCTCCAGCATAAGCCATTGACAAAGTTGCAGAACCCTTTCCAGCCTTGGCAGTCACAACTTCTGTTCCTCCATCTTGAGTCCTCTTTGTTAGAGCCTCAATGACATCAGCATTCAGATTGGCAGAAGGTGTTGCCTGAGAAAATAGTGGAAGAATAGTAACTCCTGCATGGCCTCCTATGACAGGTACATTCACATCGGCAACTGGAACTTTAGCTTTCCCAGCATAGAAAGTTTTGGCCCTAACCACATCAAGGGTTGTAACCCCAAACAATCTCTTTTCGTCGTATGTTCCTGCCTTCTTGAAAACCTCTGCAGCAATGGGAACTGTGGAGTTCACAGGGTTGCTTATCATGTTAACAAGGGCATGGGGGCAGTACTTAGCAATAGCAGTGGCAAGTGACTTGACAATGCCGGCATTAATGTTGAAAAGATCATCACGAGTCATTCCAGGCTTTCTTGGCACACCAGCAGGAATAATAACAACATCAGCACCCTCCAAAGCTTTACCAAGCTCTTCCTCACCTGCGTACCCAGCAACCTCAGATCTGGAGTTGATGTGGCTAACATCGGCGGCAACACCGGGGGTTCCAGCGATATCGTAAAGAGAGTTAGGGTTAGAAACGGAGGAGGGTTGAGCTTAAACAAATAACAAGAAAGAGAGAGGGCTGACCGATACCGCCGGCAGCGCCGAGATCGGCCACCTTGCGTTCGTGGGCCGGTTCGGGTGG
TARGET=198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7744
PRIMER_LEFT_PENALTY=0.462203
PRIMER_RIGHT_PENALTY=0.312177
PRIMER_LEFT_SEQUENCE=TGGAAAGGCCAATCTAAAACTG
PRIMER_RIGHT_SEQUENCE=TCAAATTTGCCTCCCAGTAATC
PRIMER_LEFT=100,22
PRIMER_RIGHT=349,22
PRIMER_LEFT_TM=60.462
PRIMER_RIGHT_TM=60.312
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=0.7744
PRIMER_LEFT_1_PENALTY=0.462203
PRIMER_RIGHT_1_PENALTY=0.312177
PRIMER_LEFT_1_SEQUENCE=TGGAAAGGCCAATCTAAAACTG
PRIMER_RIGHT_1_SEQUENCE=GAATCAAATTTGCCTCCCAGTA
PRIMER_LEFT_1=100,22
PRIMER_RIGHT_1=352,22
PRIMER_LEFT_1_TM=60.462
PRIMER_RIGHT_1_TM=60.312
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.9634
PRIMER_LEFT_2_PENALTY=0.462203
PRIMER_RIGHT_2_PENALTY=0.501224
PRIMER_LEFT_2_SEQUENCE=TGGAAAGGCCAATCTAAAACTG
PRIMER_RIGHT_2_SEQUENCE=GTGATACATTTCCAGCAGGTTG
PRIMER_LEFT_2=100,22
PRIMER_RIGHT_2=321,22
PRIMER_LEFT_2_TM=60.462
PRIMER_RIGHT_2_TM=59.499
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=1.0858
PRIMER_LEFT_3_PENALTY=0.462203
PRIMER_RIGHT_3_PENALTY=0.623595
PRIMER_LEFT_3_SEQUENCE=TGGAAAGGCCAATCTAAAACTG
PRIMER_RIGHT_3_SEQUENCE=ATTTGCCTCCCAGTAATCAAAC
PRIMER_LEFT_3=100,22
PRIMER_RIGHT_3=345,22
PRIMER_LEFT_3_TM=60.462
PRIMER_RIGHT_3_TM=59.376
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=1.1793
PRIMER_LEFT_4_PENALTY=0.867098
PRIMER_RIGHT_4_PENALTY=0.312177
PRIMER_LEFT_4_SEQUENCE=AACCATACTATTGGTGAAGGGG
PRIMER_RIGHT_4_SEQUENCE=TCAAATTTGCCTCCCAGTAATC
PRIMER_LEFT_4=67,22
PRIMER_RIGHT_4=349,22
PRIMER_LEFT_4_TM=59.133
PRIMER_RIGHT_4_TM=60.312
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_03500 Ps_contig_mira-and-tgicl-ass_franssen_30184
SEQUENCE=CAACGCAGAGTNCGNGGCAATTTAATGGCCGTAAGACAATTCCAATTCAACTTCTCGCGCGATAGATTGGTCTTTCTCTTTCGGACATCTCAATCTCATTCCTTCTTTCGTAATAATAAATCCCCACAATCTTTTATCTCTTTTCCCTCTTCTTTCTTCCTCCGCCGCCTAGCTGCGTCCGCAGCAACTCCGCCACCGCCTCAAGACCATCGCCGCCGTCCGCGGCTTCCTCCTCCTGACGACATGGCGCAGAAATTCGGAAAATCCACTCGCCGCCCCGGCGCTTCCTCCAAGGCTAGGGTTTACGCCGATATCAACGTCGTTCGCCCTAAGGAATATTGGGACTACGAAACCCTCACCGTTCAGTGGGGGGAGCAGGATGATTATGAGGTGGTGAGGAAGGTCGGTAGGGGGAAATACAGTGAGGTGTTTGAAGGGGTTCACTGTACTGATAATGAAAAATGTGTTATTAAGATCCTCAAACCTGTGAAGAAGAAGAAGATCAAGAGAGAAATCAAAATATTACAGAATCTTTGCGGAGGGCCCAATATTGTTAAGTTGCTTGACATTGTTAGAGACCAGCAGTCGAAGACCCCAAGTCTCATATTTGAATATGTCAATAACACTGATTTTAAAGTACTTTATCCTTTATTGTCAGACTATGACATACGATACTATATCTATGAACTTCTAAAGGCCTTGGATTACTGCCATTCACAAGGAATCATGCATCGGGATGTAAAGCCCCACAATGTAATGATTGACCATGCTCAGCGTAAGCTTCGCCTTATTGATTGGGGGCTTGCAGAATTCTATCATCCTGGGAAAGAATATAATGTTCGTGTGGCTTCAAGATATTTCAAAGGTCCGGAGCTTCTTGTTGATCTACAAGACTATGATTACTCTCTAGATTTGTGGAGTCTTGGTTGTATGTTTGCTGGGATTATATTCCGGAAGGAGCCGTTCTTTTATGGACACGATAACTATGATCAACTGGTCAAGATAGCTAGGGTACTTGGGACAGATGAATTAAATGCATATTTGAATAAGTATCGTATTGAGTTGGACCCACATCTTGCAGCACTTATTGGGAGGCACAGCAGAAAGCCATGGCAAAGGTTCATTAATGTTGAAAATCAACACTTGACTGTTCCTGAGGCTGTTGACTTTGTTGACAAGTTATTACGATACGATCACCAAGAACGCCCCACTGCAAAGGAAGCCATGGCCCATCCATACTTCAATCCAATTAGAAGTGCAGAAAGTAGTAGAGCTCGCGCACATTGAGCTACTGATGCTTGTGGAGATTGTCTTTGCGAACATTGAGCTGCTGATGCTTGTGGAGATTTATGTTGTTCGTCTTTGATTGGGGGCTTTATCTGAACGGTTGAACTTTTATTTGTGGTGGAACTTGCTTTTGGGAGATTCTGCCCCTTTCTGTTGTAAAATTCAATTTGATTAGCTCTTTTGCCCTACTTTACTTGCCTTTGGTTTCTTATTTTGTAATTTATTTGTGGAATATCATGGTCTCAATTCAACAATTTCATTTGATTGCGTGCTGAGTTTGAAAATGAAATCTTGATTCAATGAAGTNAAAYTTTATGTTATTTTCTTTTCTTTTTTAA
TARGET=489,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03501 Ps_contig_mira-and-tgicl-ass_franssen_30189
SEQUENCE=TACGCGGGGATTACAAAATAGATTCCTTCCTTATTGAGTTGGACCTACTTCATATTAGAATAGTATATCCAGGGAACCGGAAATGGTGAAAAAATGTATTTTTATTAAGTTATTAAATCGATTAAATTAAATTATTAATATAATTCTCTTCACTTCACTCATTTATATCTGAACAGAACCCAATCATCACTCTGAACCATGGCTGCTTTACAGTTTCTACTCAAGAGATCAGCTAAGCCTAACCCTTCCGATTTTCTCGCATGTAGACCTTTCTCTTCTCTCGGATTCTCCTCCTCCTCCGCCGCCGCTACCGCCACGCAAAACATCACTACTCCACCGCAGATTCCGCCGTTCGATCACCAGCCTCATCCCTATACAGGACCAACCGCCGATCAAGTATTCGCTAAGCGCAAAACGTTCCTCGGTCCTTCTTTATTCCACTACTATCAGAAACCACTGAATATTGTGGAGGGGAAGATGCAATATCTGTATGATGAGAGTGGAAGGCGATACCTTGATGCTTTTGCTGGGATAGTTACTGTTTCTTGTGGACATTGTCATCCTGAAATTTTGAATGCTATCTTTGAGCAGAGTAAACTTTTACAGCATGCAACTACTATTTATCTACACCACACTATAGCCGAGTTTGCCGAAGCCTTGGCAGCGAAAATGCCTGGAAATCTTAAAGTTGTTTATTTTGTAAATTCTGGTTCTGAAGCAAATGAATTGGCAATGCTGATGGCTAGGYTGTATACTGGTAATCTTGGTATGATTTCTTTAAGGAATGCATATCATGGAGGAAGTTCTAGTACAATAGGTCTCACTGCTCTTAACACTTGGAAATATCCAATACCAGAGGGTGAAATTCATCATGTAATGAACCCAGATCCATACCGCGGACTCTTTGGCGCAGATGTTAATAGTTATGCCAGAGATGTCCAAGACCATATTGATTATGGAACTTCTGGAAAAGTTGCTGGATTTATAGCTGAAACAATTCAGGGAGTTGGAGGAGCTGTTGAACTGGTACCTGGGTATTTAAAACATGTCTATCAAATTGTACGCAAGGCCGGTGGTGTCTGCATTGCAGATGAAGTGCAAACTGGGTTTGGCCGCACAGGAAGCCATTATTGGGGATTTGAGACACAGGGTGTTATTCCTGATATAGTTACAATGGCAAAGGGTATTGGCAATGGTTTGCCATTAGGAGCCGTAGTTACAACGCCAGAAATAGCAAATGTGATGGCCCAGAAGATTCAATTTAACACTTTTGGTGGAAACCCTGTATGTTCTGCCGGGGGTCTTGCAGTGCTCAAGGTTCTGGATAACGAGAAGCGTCAGGCTCATTGCGCTGATATTGGTTCTCACTTGCTTGAACGTTTGAGATCACTTCAACAACGACATGACATCATTGGTGATGTGAGGGGAAGAGGCTTAATGCTTGGGGTAGAGCTTGTTACCGATCGAACAAATAAAACACCTGCAAAGGCTGAAACTGCTGTCTTGTTCGAAAAACTTAGAGAACTTGGTATTCTAGTTGGGAAAGGAGGACTGCATGGAAATGTTTTCAGGATTAAGCCACCAATGTGTTTCACTAAAGATGACGCAGATTTTGTTGTGGACGCCTTGGACTACTCTATATCGAAGTTGTGAAATGATTATGCATCCTGCTAGCATACAAAATAAAAAATCCTTAGAATAAAGGAGGACTAAAATTGACTAAGGAAGG
TARGET=288,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03502 Ps_contig_mira-and-tgicl-ass_franssen_30195
SEQUENCE=AGTACGCGGGGATTGAACAATAATTGTATATATATAATCTATATGAATCATATAGAGTCTCATCCCAGGAAATATTTTTGATTTGAAGATGGGATTAGAATCTTCTGATTCAAGTGATGACAACCTCAACAACAACCTTAGCAACCCTTTGTTACTCTCTGAATCTCATTATCCTAAAAGAACAGGGACTGTTTGGACTGCCGTTGCACACATAGTGACAGGTGTGATAGGATCTGGAGTGTTGTCTCTTCCATGGAGTATTGCACAACTTGGTTGGATTGTGGGACCATTCTCCATTCTTCTTATTGCTTCTTCAACTCTCTTTTCCGCTTTTCTACTGTGTAATACTTATCGTTCTCCTAATCCTGAATATGGTCCTCATAGAAGTGCTTCTTATCTTGATGTTGTCAATTTTAATTTAGGAGTTGGGAATGGACGATTATGTGGCCTTCTTGTGAATATATGTATTTACGGTTTTGGAATTGCCTTTGTCATTACATCATCTATCAGCTTAAGAGCAATCCAAAGCTCAATTTCTCATCATGACAAAGACAAGGAAGCAACAAGTGAATTTGCAGATGCATATTATATGCTGCTTTTTGGGATTCTCCAAATTGTTCTCTCGCAGATACCAAACTTACATGACATCAATTGGCTCTCAGTTGTCGCTGCGATGACGTCTTTTGCTTATTGTTTCATTGGAATGGGACTTGCCATCATGCAAATCATGGAAAATGGATATGCTAAGGGTAGTATTGAAGGAATCAGTACCTCTAGTGGAACTGAGAAGTTGTGGTTGATTTCCCAAGCGCTTGGTGACGTGTCATTCTCGTATCCTTTCTCGACTATTATGATGGAAATACAGGATACTTTGAAGACGCCTCCGCCAGAAAACCAAACCATGAAAAAGGCCTCAACCATATCAGTCTCTATCACATCATTCTTTTACCTCTGTTGTGGATGTGCCGGATACGCTGCATTTGGCGATAATACACCAGGAAACCTTTTAACCGGATTCGGACTCTCAAGGTTTCAATGGCTAGTGAACTTCGCTAATGCTTGTATTGTGATTCATCTAGTTGGTTCATATCAGGTATACTGCCAGCCACTGTTTGGCAATGCCGAGAGTTGGTTACGTTTTAGATTCGCTGACAGTGAATTTGTGAATCACACATATACTTTGAAACTCCCATTGCTGCCAGCTTTTGAACTAAGTTTTCTCAGCTTATCATTCAGAACTGCTTATGTTGCATCAACCGTTGGGATTGCAATGATCTTTCCTTACTTCAACCAGATTCTCGGAGTTTTAGGTAGCATAAGCTTTTGGCCATTGACCGTATATTTTCCAGTGGAAATATACTTGAGTAGGTCGAATACCGATTCGTGGACTGCTAAGTGGATTATGCTTCAAACTTTCAACATTTTTGGATTTGTATTTGGATTGTTCACTCTGGTTGGATCCATTAGAGGACTCGTTACCGAAAGATTAGGCTAAGAAGTTCAGATTGGCATCGATGAATGCGGGCATTATTCTATAGTTGTAAGTTGTTTTGCAGGTCAGGTTTTTGAAGTTGGACCTGTAGAGTGGAATAAAGAAAAGTATGACTTGTTGGACAAAATCTGAGTTGTGTAACTTTTGTTTGAAGTCTTGGTAATATGAGATTAATTTTGAATGAATGTGTTTAAGAAAACTCACTGATAGTCCTTTTGTTACTTGATGGTTAGAGAGATAAAGTTACAGAAATCTTTTAGAACAAAAACAAACACACCTCCCCAGGGTAGGGCAACATGCCCTTAAGTTGCCATTAGTTCATTTTTAGGTTAAAGTCTTTCTGCCATTCTTAGAAAGTGCAAGAGAAGTATTTAGTTTGGTTTTTATATCGATGAGAAGTACGACGTTGCAGATCCGAAATTGAAGTGGGAGTTTTCCGCTATTGAAATACACAAATATAAGGGTGGGANATATGCATTCATTTCG
TARGET=27,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5237
PRIMER_LEFT_PENALTY=2.511585
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TACGCGGGGATTGAACAATAAT
PRIMER_RIGHT_SEQUENCE=TCCAAACAGTCCCTGTTCTTTT
PRIMER_LEFT=2,22
PRIMER_RIGHT=196,22
PRIMER_LEFT_TM=62.512
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.8000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=2.7491
PRIMER_LEFT_1_PENALTY=2.511585
PRIMER_RIGHT_1_PENALTY=0.237561
PRIMER_LEFT_1_SEQUENCE=TACGCGGGGATTGAACAATAAT
PRIMER_RIGHT_1_SEQUENCE=CATGGAAGAGACAACACTCCAG
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=254,22
PRIMER_LEFT_1_TM=62.512
PRIMER_RIGHT_1_TM=59.762
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=2.9733
PRIMER_LEFT_2_PENALTY=2.511585
PRIMER_RIGHT_2_PENALTY=0.461740
PRIMER_LEFT_2_SEQUENCE=TACGCGGGGATTGAACAATAAT
PRIMER_RIGHT_2_SEQUENCE=AAGGTTGTTGTTGAGGTTGTCA
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=138,22
PRIMER_LEFT_2_TM=62.512
PRIMER_RIGHT_2_TM=59.538
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=2.9870
PRIMER_LEFT_3_PENALTY=2.511585
PRIMER_RIGHT_3_PENALTY=0.475463
PRIMER_LEFT_3_SEQUENCE=TACGCGGGGATTGAACAATAAT
PRIMER_RIGHT_3_SEQUENCE=GTCCAAACAGTCCCTGTTCTTT
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=197,22
PRIMER_LEFT_3_TM=62.512
PRIMER_RIGHT_3_TM=59.525
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=3.1544
PRIMER_LEFT_4_PENALTY=2.511585
PRIMER_RIGHT_4_PENALTY=0.642812
PRIMER_LEFT_4_SEQUENCE=TACGCGGGGATTGAACAATAAT
PRIMER_RIGHT_4_SEQUENCE=GGTTGTTGTTGAGGTTGTCATC
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=136,22
PRIMER_LEFT_4_TM=62.512
PRIMER_RIGHT_4_TM=59.357
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=135
=
PRIMER_SEQUENCE_ID=SSR_03503 Ps_contig_mira-and-tgicl-ass_franssen_30200
SEQUENCE=CTACAGTAATATGTACCAAAATAAGGTTGCAAACTTCAATCAACAGAAGCAGCAGATGAACAATCTTTATTCAATAAAACGAAGTCCATCATCATCATATACAAAATCAACATTTTATTTTCCTTCAGAAACAAACAAACTTATTTCCATTTCACTTAACCATCATGCTTCTAAAAGCTGCAAAATCTACATAACTGTGTTCTTGTCTTGTAGGACTCATGCTACTGGTTTCAGCACATACATTTTACAGGCATGTGATTCTACTGTTACACTCAACTTATCCACAAAATGTTTCTCCAATGTCTTGTGCTCCCAAAGATCTCTGGCTTCAACAACACTTTTCGGCGGTATGCCAATGTCATCCCAATTCGCTGTCATAGCCATTCGCTGCGCACCTTTATTAAGCAACACGACGGCTACCCTGTATCCTGAAAGAGGACCTGCCCATATCTCGAGATCACCTTCCATCCTAACCTTTTTAGCTTGTACACCAAGCGAATCTTGGTTAACTGCAATAACTTCTTTATTAGAGATTATTTCCATGGTATCTTTACTAACATTTCTTACATCACACCCAAGAAGAAGTGGAGCCTTTGAAATGGCCCATAAACTGAAGTGAACAATGTATTCGTTTTTCGTCATCCCTCCGTTTCCAACCTCGAGCATATCAGGATCATTCCAGCCACCAGGCCGTGCTAGCTCGGCATAAACTTCATTCGTGTCCGCCTTTGAAATCATACTTTCCCATGTATCACTTATGTCACCGGTAGTTCTCCAACTATTTCCGACTTTAGCACCCCATAAAGCTGGGTGCAAATCTCCCCATTCACATAGCGAAAAGAAGATCGGACGACCTGCCTTCACTAAAGCCCGCGTCATAACAGGATACCTCTTGGTGGGCTTTGATCCACCATTGAAACAGTTATCATACTTCAAATAATCAATGCCCCATGATGCAAAAGTTTTGGCATCTTGGAACTCATGACCTAGTGAACCAGGCATTTGTTTGCTACAGGTAAAATACCCTGCATCTGAATAAATTCCAAGCTTAAGACCCTTGCTGTGAACATAATCTGCAAGCGCTTTAATACCAGAAGGAAATGTTGAGTTCTTAGCCACCAGATTACCCTTATCATCGCGATTAAGTTCAGCCCAACAATCATCTATGTTGACATAGGTGTATCCAAGCTTAGATAGACCAGTAGAAATAAGGGCATCAGCAGTTTCTCTGATCATTTTTTCATCAATCTGACAGGCGAAGTGATTCCAACTGTTCCACCCCATAGGAGGAGTTCTAGCAAGACCATTAGCAAGAAGGTTTCTTCTAAGAAGATGCTCCTCCTCATTGTTCTTGTTGTTAGCTAATGATGAACAAGTGACACAGATAAGCAAAAGGGTCACCAAAACCACCATCATCTCAATCTTTATCCCCATTTTCGACACTCTTTACCAGAAACTATACTTCACTTCACTTTCTCAGTTGAAGAGAGAGAGAGAGAGAGAGAGGCGGTCCTTATTCTTTCGTTACGTTACCCGTCCCGGTTACCG
TARGET=87,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0267
PRIMER_LEFT_PENALTY=0.022302
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=TCAATCAACAGAAGCAGCAGAT
PRIMER_RIGHT_SEQUENCE=ATCTTTGGGAGCACAAGACATT
PRIMER_LEFT=35,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=60.022
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.0267
PRIMER_LEFT_1_PENALTY=0.022302
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=CAGAAGCAGCAGATGAACAATC
PRIMER_RIGHT_1_SEQUENCE=ATCTTTGGGAGCACAAGACATT
PRIMER_LEFT_1=43,22
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=60.022
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=277
PRIMER_PAIR_PENALTY_2=0.0267
PRIMER_LEFT_2_PENALTY=0.022302
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=ATCAACAGAAGCAGCAGATGAA
PRIMER_RIGHT_2_SEQUENCE=ATCTTTGGGAGCACAAGACATT
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=319,22
PRIMER_LEFT_2_TM=60.022
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.0637
PRIMER_LEFT_3_PENALTY=0.059316
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=AACAGAAGCAGCAGATGAACAA
PRIMER_RIGHT_3_SEQUENCE=ATCTTTGGGAGCACAAGACATT
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=319,22
PRIMER_LEFT_3_TM=60.059
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.0637
PRIMER_LEFT_4_PENALTY=0.059316
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=ACTTCAATCAACAGAAGCAGCA
PRIMER_RIGHT_4_SEQUENCE=ATCTTTGGGAGCACAAGACATT
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=319,22
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_03504 Ps_contig_mira-and-tgicl-ass_franssen_30200
SEQUENCE=CTACAGTAATATGTACCAAAATAAGGTTGCAAACTTCAATCAACAGAAGCAGCAGATGAACAATCTTTATTCAATAAAACGAAGTCCATCATCATCATATACAAAATCAACATTTTATTTTCCTTCAGAAACAAACAAACTTATTTCCATTTCACTTAACCATCATGCTTCTAAAAGCTGCAAAATCTACATAACTGTGTTCTTGTCTTGTAGGACTCATGCTACTGGTTTCAGCACATACATTTTACAGGCATGTGATTCTACTGTTACACTCAACTTATCCACAAAATGTTTCTCCAATGTCTTGTGCTCCCAAAGATCTCTGGCTTCAACAACACTTTTCGGCGGTATGCCAATGTCATCCCAATTCGCTGTCATAGCCATTCGCTGCGCACCTTTATTAAGCAACACGACGGCTACCCTGTATCCTGAAAGAGGACCTGCCCATATCTCGAGATCACCTTCCATCCTAACCTTTTTAGCTTGTACACCAAGCGAATCTTGGTTAACTGCAATAACTTCTTTATTAGAGATTATTTCCATGGTATCTTTACTAACATTTCTTACATCACACCCAAGAAGAAGTGGAGCCTTTGAAATGGCCCATAAACTGAAGTGAACAATGTATTCGTTTTTCGTCATCCCTCCGTTTCCAACCTCGAGCATATCAGGATCATTCCAGCCACCAGGCCGTGCTAGCTCGGCATAAACTTCATTCGTGTCCGCCTTTGAAATCATACTTTCCCATGTATCACTTATGTCACCGGTAGTTCTCCAACTATTTCCGACTTTAGCACCCCATAAAGCTGGGTGCAAATCTCCCCATTCACATAGCGAAAAGAAGATCGGACGACCTGCCTTCACTAAAGCCCGCGTCATAACAGGATACCTCTTGGTGGGCTTTGATCCACCATTGAAACAGTTATCATACTTCAAATAATCAATGCCCCATGATGCAAAAGTTTTGGCATCTTGGAACTCATGACCTAGTGAACCAGGCATTTGTTTGCTACAGGTAAAATACCCTGCATCTGAATAAATTCCAAGCTTAAGACCCTTGCTGTGAACATAATCTGCAAGCGCTTTAATACCAGAAGGAAATGTTGAGTTCTTAGCCACCAGATTACCCTTATCATCGCGATTAAGTTCAGCCCAACAATCATCTATGTTGACATAGGTGTATCCAAGCTTAGATAGACCAGTAGAAATAAGGGCATCAGCAGTTTCTCTGATCATTTTTTCATCAATCTGACAGGCGAAGTGATTCCAACTGTTCCACCCCATAGGAGGAGTTCTAGCAAGACCATTAGCAAGAAGGTTTCTTCTAAGAAGATGCTCCTCCTCATTGTTCTTGTTGTTAGCTAATGATGAACAAGTGACACAGATAAGCAAAAGGGTCACCAAAACCACCATCATCTCAATCTTTATCCCCATTTTCGACACTCTTTACCAGAAACTATACTTCACTTCACTTTCTCAGTTGAAGAGAGAGAGAGAGAGAGAGAGGCGGTCCTTATTCTTTCGTTACGTTACCCGTCCCGGTTACCG
TARGET=129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0267
PRIMER_LEFT_PENALTY=0.022302
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=TCAATCAACAGAAGCAGCAGAT
PRIMER_RIGHT_SEQUENCE=ATCTTTGGGAGCACAAGACATT
PRIMER_LEFT=35,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=60.022
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.0267
PRIMER_LEFT_1_PENALTY=0.022302
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=CAGAAGCAGCAGATGAACAATC
PRIMER_RIGHT_1_SEQUENCE=ATCTTTGGGAGCACAAGACATT
PRIMER_LEFT_1=43,22
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=60.022
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=277
PRIMER_PAIR_PENALTY_2=0.0267
PRIMER_LEFT_2_PENALTY=0.022302
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=ATCAACAGAAGCAGCAGATGAA
PRIMER_RIGHT_2_SEQUENCE=ATCTTTGGGAGCACAAGACATT
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=319,22
PRIMER_LEFT_2_TM=60.022
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.0637
PRIMER_LEFT_3_PENALTY=0.059316
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=AACAGAAGCAGCAGATGAACAA
PRIMER_RIGHT_3_SEQUENCE=ATCTTTGGGAGCACAAGACATT
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=319,22
PRIMER_LEFT_3_TM=60.059
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.0637
PRIMER_LEFT_4_PENALTY=0.059316
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=ACTTCAATCAACAGAAGCAGCA
PRIMER_RIGHT_4_SEQUENCE=ATCTTTGGGAGCACAAGACATT
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=319,22
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_03505 Ps_contig_mira-and-tgicl-ass_franssen_30200
SEQUENCE=CTACAGTAATATGTACCAAAATAAGGTTGCAAACTTCAATCAACAGAAGCAGCAGATGAACAATCTTTATTCAATAAAACGAAGTCCATCATCATCATATACAAAATCAACATTTTATTTTCCTTCAGAAACAAACAAACTTATTTCCATTTCACTTAACCATCATGCTTCTAAAAGCTGCAAAATCTACATAACTGTGTTCTTGTCTTGTAGGACTCATGCTACTGGTTTCAGCACATACATTTTACAGGCATGTGATTCTACTGTTACACTCAACTTATCCACAAAATGTTTCTCCAATGTCTTGTGCTCCCAAAGATCTCTGGCTTCAACAACACTTTTCGGCGGTATGCCAATGTCATCCCAATTCGCTGTCATAGCCATTCGCTGCGCACCTTTATTAAGCAACACGACGGCTACCCTGTATCCTGAAAGAGGACCTGCCCATATCTCGAGATCACCTTCCATCCTAACCTTTTTAGCTTGTACACCAAGCGAATCTTGGTTAACTGCAATAACTTCTTTATTAGAGATTATTTCCATGGTATCTTTACTAACATTTCTTACATCACACCCAAGAAGAAGTGGAGCCTTTGAAATGGCCCATAAACTGAAGTGAACAATGTATTCGTTTTTCGTCATCCCTCCGTTTCCAACCTCGAGCATATCAGGATCATTCCAGCCACCAGGCCGTGCTAGCTCGGCATAAACTTCATTCGTGTCCGCCTTTGAAATCATACTTTCCCATGTATCACTTATGTCACCGGTAGTTCTCCAACTATTTCCGACTTTAGCACCCCATAAAGCTGGGTGCAAATCTCCCCATTCACATAGCGAAAAGAAGATCGGACGACCTGCCTTCACTAAAGCCCGCGTCATAACAGGATACCTCTTGGTGGGCTTTGATCCACCATTGAAACAGTTATCATACTTCAAATAATCAATGCCCCATGATGCAAAAGTTTTGGCATCTTGGAACTCATGACCTAGTGAACCAGGCATTTGTTTGCTACAGGTAAAATACCCTGCATCTGAATAAATTCCAAGCTTAAGACCCTTGCTGTGAACATAATCTGCAAGCGCTTTAATACCAGAAGGAAATGTTGAGTTCTTAGCCACCAGATTACCCTTATCATCGCGATTAAGTTCAGCCCAACAATCATCTATGTTGACATAGGTGTATCCAAGCTTAGATAGACCAGTAGAAATAAGGGCATCAGCAGTTTCTCTGATCATTTTTTCATCAATCTGACAGGCGAAGTGATTCCAACTGTTCCACCCCATAGGAGGAGTTCTAGCAAGACCATTAGCAAGAAGGTTTCTTCTAAGAAGATGCTCCTCCTCATTGTTCTTGTTGTTAGCTAATGATGAACAAGTGACACAGATAAGCAAAAGGGTCACCAAAACCACCATCATCTCAATCTTTATCCCCATTTTCGACACTCTTTACCAGAAACTATACTTCACTTCACTTTCTCAGTTGAAGAGAGAGAGAGAGAGAGAGAGGCGGTCCTTATTCTTTCGTTACGTTACCCGTCCCGGTTACCG
TARGET=1485,22
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0179
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.017127
PRIMER_LEFT_SEQUENCE=AGATAAGCAAAAGGGTCACCAA
PRIMER_RIGHT_SEQUENCE=CGTAACGAAAGAATAAGGACCG
PRIMER_LEFT=1382,22
PRIMER_RIGHT=1528,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.017
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=147
PRIMER_PAIR_PENALTY_1=0.0292
PRIMER_LEFT_1_PENALTY=0.012045
PRIMER_RIGHT_1_PENALTY=0.017127
PRIMER_LEFT_1_SEQUENCE=ACCCCATAGGAGGAGTTCTAGC
PRIMER_RIGHT_1_SEQUENCE=CGTAACGAAAGAATAAGGACCG
PRIMER_LEFT_1=1277,22
PRIMER_RIGHT_1=1528,22
PRIMER_LEFT_1_TM=59.988
PRIMER_RIGHT_1_TM=60.017
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.1042
PRIMER_LEFT_2_PENALTY=0.087031
PRIMER_RIGHT_2_PENALTY=0.017127
PRIMER_LEFT_2_SEQUENCE=AGCAAGACCATTAGCAAGAAGG
PRIMER_RIGHT_2_SEQUENCE=CGTAACGAAAGAATAAGGACCG
PRIMER_LEFT_2=1296,22
PRIMER_RIGHT_2=1528,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=60.017
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.1457
PRIMER_LEFT_3_PENALTY=0.128591
PRIMER_RIGHT_3_PENALTY=0.017127
PRIMER_LEFT_3_SEQUENCE=CCCATTTTCGACACTCTTTACC
PRIMER_RIGHT_3_SEQUENCE=CGTAACGAAAGAATAAGGACCG
PRIMER_LEFT_3=1429,22
PRIMER_RIGHT_3=1528,22
PRIMER_LEFT_3_TM=59.871
PRIMER_RIGHT_3_TM=60.017
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=0.1457
PRIMER_LEFT_4_PENALTY=0.128591
PRIMER_RIGHT_4_PENALTY=0.017127
PRIMER_LEFT_4_SEQUENCE=CCCCATTTTCGACACTCTTTAC
PRIMER_RIGHT_4_SEQUENCE=CGTAACGAAAGAATAAGGACCG
PRIMER_LEFT_4=1428,22
PRIMER_RIGHT_4=1528,22
PRIMER_LEFT_4_TM=59.871
PRIMER_RIGHT_4_TM=60.017
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_03506 Ps_contig_mira-and-tgicl-ass_franssen_30202
SEQUENCE=GTAAGGTTGACTATTTCTCTGTCTGATGTAGCAAANAGACAAAACACATTCTACTTTTCTTTTTTAACTTGGCTTGTTGAAACAAAACCAAATGACTCNATGAATCACCATCTATAAAAGAGCTAGTTTTGGTTTATCGGGAGATAAGCTAAGATGACAGAGGTGAAACTGCATGGATTTTGGTATAGTCCTTTTACTTTTAGGGTAGTATGGACCCTAAATCTGAAGGGTATACCATATGAGCACATAGAAGAAGATCGTTTCAATAAGAGTCCTCAACTTCTTCAATATAACCCAGTATACAAAAAGACTCCAGTGCTAGTTCATGATGGAAAACCCATATGTGAATCCATGCTCATTGTTGAATACATTGATGAGATATGGCCACAGAATTCATTGCTTCCTGCTGATTCATATGACAGAGCTCAAGCACGGTTTTGGGTTAAATATGTTGATGACATGTTTTCAGGAATTGCGGCGCACTTTCGTAGCAGAAGCGATGAAGAGAGAGAGGAGGCGAAAGAGATTATATGGGAGCGACTCGGAGTTGTTGAGGATCTGTGTCTGGGAGATGAGAGGAAATTCTATGGGGGAGATGCCATTAACATTGTAGACATAGCTCTTGGATCTTTTGTCAAGTTTATTGAGGTTCAAGAGGATATGTTTGATGTGAAGATCTTGCAAAGTGAGAGGTTTCCTCGCTTGAAATTATGGTTTAATAATTTCAAGGATGTTCCGATCATTAAAGAAAATACCCCTGGTCAAGAGGAGTTGGTGGCTTTTTTAAAGACTCTTATTGAAAAAATGATGGCAAGGAAATAGTGGCTTCTTGGATTGATATATCATCATGAGTCATCTTGTTATGTGTGTTTGCTTGTGTTGTTTTGTTAGTCAATAATCAAAGATTAAAATGGTTTCCTTTGTTTAATATTAAGAGTATGTACCACCACACGTCTAATAAAGTTATAGATAAGTTGATTCCTAACATCTTACTTTTGTTTTCCACCAAAATTGATGTATTTGGTACACAAGATTACTTCATAAGTCTAACTAATTGTCTTTAATAAGAGCTTAAACAAGAATGTTATAGTCCGAAATAAATATTTTATTCATT
TARGET=504,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2326
PRIMER_LEFT_PENALTY=0.158697
PRIMER_RIGHT_PENALTY=0.073866
PRIMER_LEFT_SEQUENCE=GCTAGTTCATGATGGAAAACCC
PRIMER_RIGHT_SEQUENCE=ATTTCCTCTCATCTCCCAGACA
PRIMER_LEFT=317,22
PRIMER_RIGHT=582,22
PRIMER_LEFT_TM=59.841
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.2342
PRIMER_LEFT_1_PENALTY=0.158697
PRIMER_RIGHT_1_PENALTY=0.075553
PRIMER_LEFT_1_SEQUENCE=GCTAGTTCATGATGGAAAACCC
PRIMER_RIGHT_1_SEQUENCE=CGCTCCCATATAATCTCTTTCG
PRIMER_LEFT_1=317,22
PRIMER_RIGHT_1=538,22
PRIMER_LEFT_1_TM=59.841
PRIMER_RIGHT_1_TM=60.076
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.2405
PRIMER_LEFT_2_PENALTY=0.158697
PRIMER_RIGHT_2_PENALTY=0.081801
PRIMER_LEFT_2_SEQUENCE=GCTAGTTCATGATGGAAAACCC
PRIMER_RIGHT_2_SEQUENCE=CGAGTCGCTCCCATATAATCTC
PRIMER_LEFT_2=317,22
PRIMER_RIGHT_2=543,22
PRIMER_LEFT_2_TM=59.841
PRIMER_RIGHT_2_TM=60.082
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=0.3042
PRIMER_LEFT_3_PENALTY=0.230329
PRIMER_RIGHT_3_PENALTY=0.073866
PRIMER_LEFT_3_SEQUENCE=CATTGCTTCCTGCTGATTCATA
PRIMER_RIGHT_3_SEQUENCE=ATTTCCTCTCATCTCCCAGACA
PRIMER_LEFT_3=394,22
PRIMER_RIGHT_3=582,22
PRIMER_LEFT_3_TM=60.230
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.3059
PRIMER_LEFT_4_PENALTY=0.230329
PRIMER_RIGHT_4_PENALTY=0.075553
PRIMER_LEFT_4_SEQUENCE=CATTGCTTCCTGCTGATTCATA
PRIMER_RIGHT_4_SEQUENCE=CGCTCCCATATAATCTCTTTCG
PRIMER_LEFT_4=394,22
PRIMER_RIGHT_4=538,22
PRIMER_LEFT_4_TM=60.230
PRIMER_RIGHT_4_TM=60.076
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=9.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_03507 Ps_contig_mira-and-tgicl-ass_franssen_30219
SEQUENCE=AACCTTAAAATTAATAGTTGAATACTTAAATATCATTTTTCATAAACATGAAATATTTTCTTCTTCTTATAAATAAGGTCCAAATAGTAAAACACACACTAACTCGCACTTGACCAGAGTATTACAACAAATAAGGAAACCAAATACATGCACCACATCCACACATCCTACATAATCATCATCTATGGAAAAACACACATTAATTTCCACAAATGTTATTAGCTGTTCCATCATTGAACCCGCCAAGTATTCCACTGAACCAAGATTATTAGAAAACTCAACCAATCCATCATGCTTCATTTGCATCGAAGGCTGGCACTATACTACAGGTTTGCAATACAGCTAATTCTTTTCAGATAAAGCCTATACTTCTGGAGATGGCTGGCTACGTCCTCCCTTGTAATATTTTCGACATTCATGGTATTGAGAACTTTTTTTTGGTACCGCCTTGTCAATGGCTAGGTGATTAACAGCAGCGACAAACTTGCGGTGCAATTCGACAGACCAAACAACTCCTCGGGGGTTCTTCCTCCTAATTACATGCTGCCAAAGGTTCTTTAACTCCTCTAATCTCACAGGTTTCAGTAGATAATCACAGGCACCGTGGGAAATTCCCTTCATCACCAGCTTCGTATCATGACATGCAGACAACATTATGACAGGAAGGTTCATCTCAACTCCCACTAGTTCAAGGAGCTTAAATCCATCCATGTCTGGTATGTGCACATCAATGATTACCAGATCAAAGTTGTCTTTGTTTTCTTGCAACATAGCCAATGCTGTTATTCCTTCATTAGTCGTGGTTACATGGTATTGATATGTTTGGAGAAGATTTTCTAAAACTTGAAGGCAAGTGGGATCATGGTCAACTGCAAGAATACGCATACCGATAGGAAACAGGTAATTGGAATCATCCATTAGTGTTCTCCACTATCTATTCACTCACTCACACAGTATTGTTTTGTAATGAGAATGAATGAATGAATAATAATTGGAGGATAAGATATGGGGTTGGTGTGTTATTGTTTACGCGAGAGTGGAATCTGCACTAATTTAAAAAGTGGAGTGGATGTAGTAAAGAAGCTCTCCCCC
TARGET=939,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1830
PRIMER_LEFT_PENALTY=0.155310
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=CTAAAACTTGAAGGCAAGTGGG
PRIMER_RIGHT_SEQUENCE=ATTCCACTCTCGCGTAAACAAT
PRIMER_LEFT=836,22
PRIMER_RIGHT=1042,22
PRIMER_LEFT_TM=60.155
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.1892
PRIMER_LEFT_1_PENALTY=0.155310
PRIMER_RIGHT_1_PENALTY=0.033905
PRIMER_LEFT_1_SEQUENCE=CTAAAACTTGAAGGCAAGTGGG
PRIMER_RIGHT_1_SEQUENCE=CAACCCCATATCTTATCCTCCA
PRIMER_LEFT_1=836,22
PRIMER_RIGHT_1=1013,22
PRIMER_LEFT_1_TM=60.155
PRIMER_RIGHT_1_TM=60.034
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.2961
PRIMER_LEFT_2_PENALTY=0.268401
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=CCGATAGGAAACAGGTAATTGG
PRIMER_RIGHT_2_SEQUENCE=ATTCCACTCTCGCGTAAACAAT
PRIMER_LEFT_2=886,22
PRIMER_RIGHT_2=1042,22
PRIMER_LEFT_2_TM=59.732
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.3023
PRIMER_LEFT_3_PENALTY=0.268401
PRIMER_RIGHT_3_PENALTY=0.033905
PRIMER_LEFT_3_SEQUENCE=CCGATAGGAAACAGGTAATTGG
PRIMER_RIGHT_3_SEQUENCE=CAACCCCATATCTTATCCTCCA
PRIMER_LEFT_3=886,22
PRIMER_RIGHT_3=1013,22
PRIMER_LEFT_3_TM=59.732
PRIMER_RIGHT_3_TM=60.034
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=128
PRIMER_PAIR_PENALTY_4=0.3536
PRIMER_LEFT_4_PENALTY=0.325885
PRIMER_RIGHT_4_PENALTY=0.027715
PRIMER_LEFT_4_SEQUENCE=CAACTGCAAGAATACGCATACC
PRIMER_RIGHT_4_SEQUENCE=ATTCCACTCTCGCGTAAACAAT
PRIMER_LEFT_4=866,22
PRIMER_RIGHT_4=1042,22
PRIMER_LEFT_4_TM=59.674
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.8000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_03508 Ps_contig_mira-and-tgicl-ass_franssen_30219
SEQUENCE=AACCTTAAAATTAATAGTTGAATACTTAAATATCATTTTTCATAAACATGAAATATTTTCTTCTTCTTATAAATAAGGTCCAAATAGTAAAACACACACTAACTCGCACTTGACCAGAGTATTACAACAAATAAGGAAACCAAATACATGCACCACATCCACACATCCTACATAATCATCATCTATGGAAAAACACACATTAATTTCCACAAATGTTATTAGCTGTTCCATCATTGAACCCGCCAAGTATTCCACTGAACCAAGATTATTAGAAAACTCAACCAATCCATCATGCTTCATTTGCATCGAAGGCTGGCACTATACTACAGGTTTGCAATACAGCTAATTCTTTTCAGATAAAGCCTATACTTCTGGAGATGGCTGGCTACGTCCTCCCTTGTAATATTTTCGACATTCATGGTATTGAGAACTTTTTTTTGGTACCGCCTTGTCAATGGCTAGGTGATTAACAGCAGCGACAAACTTGCGGTGCAATTCGACAGACCAAACAACTCCTCGGGGGTTCTTCCTCCTAATTACATGCTGCCAAAGGTTCTTTAACTCCTCTAATCTCACAGGTTTCAGTAGATAATCACAGGCACCGTGGGAAATTCCCTTCATCACCAGCTTCGTATCATGACATGCAGACAACATTATGACAGGAAGGTTCATCTCAACTCCCACTAGTTCAAGGAGCTTAAATCCATCCATGTCTGGTATGTGCACATCAATGATTACCAGATCAAAGTTGTCTTTGTTTTCTTGCAACATAGCCAATGCTGTTATTCCTTCATTAGTCGTGGTTACATGGTATTGATATGTTTGGAGAAGATTTTCTAAAACTTGAAGGCAAGTGGGATCATGGTCAACTGCAAGAATACGCATACCGATAGGAAACAGGTAATTGGAATCATCCATTAGTGTTCTCCACTATCTATTCACTCACTCACACAGTATTGTTTTGTAATGAGAATGAATGAATGAATAATAATTGGAGGATAAGATATGGGGTTGGTGTGTTATTGTTTACGCGAGAGTGGAATCTGCACTAATTTAAAAAGTGGAGTGGATGTAGTAAAGAAGCTCTCCCCC
TARGET=971,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1830
PRIMER_LEFT_PENALTY=0.155310
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=CTAAAACTTGAAGGCAAGTGGG
PRIMER_RIGHT_SEQUENCE=ATTCCACTCTCGCGTAAACAAT
PRIMER_LEFT=836,22
PRIMER_RIGHT=1042,22
PRIMER_LEFT_TM=60.155
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.1892
PRIMER_LEFT_1_PENALTY=0.155310
PRIMER_RIGHT_1_PENALTY=0.033905
PRIMER_LEFT_1_SEQUENCE=CTAAAACTTGAAGGCAAGTGGG
PRIMER_RIGHT_1_SEQUENCE=CAACCCCATATCTTATCCTCCA
PRIMER_LEFT_1=836,22
PRIMER_RIGHT_1=1013,22
PRIMER_LEFT_1_TM=60.155
PRIMER_RIGHT_1_TM=60.034
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.2961
PRIMER_LEFT_2_PENALTY=0.268401
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=CCGATAGGAAACAGGTAATTGG
PRIMER_RIGHT_2_SEQUENCE=ATTCCACTCTCGCGTAAACAAT
PRIMER_LEFT_2=886,22
PRIMER_RIGHT_2=1042,22
PRIMER_LEFT_2_TM=59.732
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.3023
PRIMER_LEFT_3_PENALTY=0.268401
PRIMER_RIGHT_3_PENALTY=0.033905
PRIMER_LEFT_3_SEQUENCE=CCGATAGGAAACAGGTAATTGG
PRIMER_RIGHT_3_SEQUENCE=CAACCCCATATCTTATCCTCCA
PRIMER_LEFT_3=886,22
PRIMER_RIGHT_3=1013,22
PRIMER_LEFT_3_TM=59.732
PRIMER_RIGHT_3_TM=60.034
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=128
PRIMER_PAIR_PENALTY_4=0.3536
PRIMER_LEFT_4_PENALTY=0.325885
PRIMER_RIGHT_4_PENALTY=0.027715
PRIMER_LEFT_4_SEQUENCE=CAACTGCAAGAATACGCATACC
PRIMER_RIGHT_4_SEQUENCE=ATTCCACTCTCGCGTAAACAAT
PRIMER_LEFT_4=866,22
PRIMER_RIGHT_4=1042,22
PRIMER_LEFT_4_TM=59.674
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.8000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_03509 Ps_contig_mira-and-tgicl-ass_franssen_30222
SEQUENCE=GATACAATTTCCCATTTCAAACCAAGCATTTAACTCATTTGACTCTACAGATATCCATTTAATTTGCAATTAACATATGGATCCAAAACTTAATAAACTTTTAGAAACTTTCTTCAAAACCAACTCAAATACAATTTTAAAAACCAATTTGGTTTCACATAAGGAGCATCTTCGTATGTTTCAAACTCTTAAAATTCCTTATACAACTACTATTACATGATCAAGAGAATTACACAATCTAACATCTATCCCTAAACCATCATCTTAATCCTCAAGTCTTGGTATCCTTGATCATATCCATTTGCCATGACATGACATTTTAATACCATGATAACAAGCTTACACAGCAGGCCAAAATCCAAATCAACCATGAGCAAGTAAACAACCTGCAGCATGCGAAACCATTTTGCAATGTGGTAACATTCATCTCAAAAATAGAATTGCCTCAAAACACTGATAACAACAGCAAGCCATACTGAGATTCTGCGTTTTCTGCAAAAAACTTCATTCTCCATTTTGCTTCACCTTCATCTTCCTGCTTCAAATCCTTCACCTTCATCTTCCTGCTTCACAATCTTCACTCTTCATCCAATCTTCAAATCCTTGAAATCTTCACTCTTCATCCACGCACAAGCTTTTTTCACTCTAAATCTTCTTCATTAATCAATCTTAACTCTCAGAGTCTCACGATTCGTAATACTCTCCGAGGAGACGAATCTCTGGAGTACTTCAGGAAACGCCACTTCTGAAGAGCCGCCGAAGCTACTCTGGCAGGTAAATAATCCTCCGCTTCTTCTTTTGATTGGATCCTGAATCGCCACCACAGTTTAGTTTTGAGCTTCAGGATTTAAAGCCATACCCTCGTAGGTTTCGATTCCCTCGCGATATCTTATGATTTCTTTTGATCTGGAAATTTAGGGTTTATTTGGGGTTTTTCTTCGATTCGGGCTTAGGGAAAGGAATCAAATGGAAACGGTGTGAGATCGAGATAGAGTGAGAAAGTATGAGTGTTTTGATGTAACATTTAGGGGGTTAGGTCATCCGCCGCCGCCGGTGGCGAATTCCAGCGCGACGGAGGGGTTGTTTCTCAGGAGAAGCAAATGAAGAACCCCCGACGTACTCTGCGTTTGTTATACCCACTGCTT
TARGET=1042,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2332
PRIMER_LEFT_PENALTY=0.179693
PRIMER_RIGHT_PENALTY=0.053515
PRIMER_LEFT_SEQUENCE=GGCTTAGGGAAAGGAATCAAAT
PRIMER_RIGHT_SEQUENCE=CAGTGGGTATAACAAACGCAGA
PRIMER_LEFT=946,22
PRIMER_RIGHT=1141,22
PRIMER_LEFT_TM=59.820
PRIMER_RIGHT_TM=60.054
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.3079
PRIMER_LEFT_1_PENALTY=0.066394
PRIMER_RIGHT_1_PENALTY=0.241544
PRIMER_LEFT_1_SEQUENCE=ATCGCCACCACAGTTTAGTTTT
PRIMER_RIGHT_1_SEQUENCE=GGTTCTTCATTTGCTTCTCCTG
PRIMER_LEFT_1=813,22
PRIMER_RIGHT_1=1109,22
PRIMER_LEFT_1_TM=59.934
PRIMER_RIGHT_1_TM=60.242
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.3079
PRIMER_LEFT_2_PENALTY=0.066394
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=AATCGCCACCACAGTTTAGTTT
PRIMER_RIGHT_2_SEQUENCE=GGTTCTTCATTTGCTTCTCCTG
PRIMER_LEFT_2=812,22
PRIMER_RIGHT_2=1109,22
PRIMER_LEFT_2_TM=59.934
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=0.3202
PRIMER_LEFT_3_PENALTY=0.078668
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=GATTCCCTCGCGATATCTTATG
PRIMER_RIGHT_3_SEQUENCE=GGTTCTTCATTTGCTTCTCCTG
PRIMER_LEFT_3=872,22
PRIMER_RIGHT_3=1109,22
PRIMER_LEFT_3_TM=59.921
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.5990
PRIMER_LEFT_4_PENALTY=0.545451
PRIMER_RIGHT_4_PENALTY=0.053515
PRIMER_LEFT_4_SEQUENCE=GGATTTAAAGCCATACCCTCGT
PRIMER_RIGHT_4_SEQUENCE=CAGTGGGTATAACAAACGCAGA
PRIMER_LEFT_4=843,22
PRIMER_RIGHT_4=1141,22
PRIMER_LEFT_4_TM=60.545
PRIMER_RIGHT_4_TM=60.054
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_03510 Ps_contig_mira-and-tgicl-ass_franssen_30224
SEQUENCE=AGTTCTTCTTCTTCTCCTCTTTTTTTTTTAATTTCAATTTCTTCGTCTCGAAGCTTCTTCCATTCCGATCGCTCCAATTTCACTTCAATGGCTACGATCGGACACAGCAACCTCAACGCCAAATTGGTTCTGCTTGGGGATATGGGTGCTGGAAAATCAAGCCTTGTATTGCGTTTTGTCAAGGGTCAATTCCTTGAGTTTCAGGAATCAACAATAGGGGCAGCATTTTTCTCCCAGACGTTGGCAGTCAATGACGCTACAGTGAAATTTGAGATTTGGGACACAGCAGGACAAGAGAGATACCATAGCTTGGCCCCCATGTACTATAGAGGCGCTGCTGCTGCTATCATTGTCTATGACATTACTAACTTGGATTCATTTACACGGGCGAAGAAATGGGTCCAAGAGCTTCAGAAACAAGGGAATCCTAACATGGTCGTGGCTTTTGCCGGTAACAAAGCTGACTTGGAAGATAAAAGGAAAGTGACAGCTGAAGAAGCACGTGTATATGCTGAGGAAAATGGTCTGTTTTTCATTGAGGCCTCTGCAAAAACTGCAGCCAATGTTAATGAAATATTTTATGAAATAGCCAAGAGGTTACCGAGGGCACAGCCAGCACAACCAGCAGGAATGGTTCTTGTTGATAGACCCGCCGAAGGATCCAGAGCTGCATCATGTTGTTCATAATTATTTATTCTGTTTGTTCCATCTTAGTTAAACTTGTTTTCTGTGTTTCTGTGTATACTATACGCACTTCGAATCTTTATGCGAAGCTCCAACAAGTTTATGGGCTGCAGATTACATAATGTAACTGGTTGGTCTGAATTATATCTCTTGGATGTTGACTTTTAGTCTGTTGATTGATCTGTTGTGTACATAGGGCGAAGTGCTTGAAAGTTGAAAGTCAAGCTCGTTTTAAAATTGTTCAAACATTCATTTTCTGAGTGCAGAAAATTCAATAAACACAAAATGAGGAGAACCTTCTAACRAG
TARGET=3,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03511 Ps_contig_mira-and-tgicl-ass_franssen_30224
SEQUENCE=AGTTCTTCTTCTTCTCCTCTTTTTTTTTTAATTTCAATTTCTTCGTCTCGAAGCTTCTTCCATTCCGATCGCTCCAATTTCACTTCAATGGCTACGATCGGACACAGCAACCTCAACGCCAAATTGGTTCTGCTTGGGGATATGGGTGCTGGAAAATCAAGCCTTGTATTGCGTTTTGTCAAGGGTCAATTCCTTGAGTTTCAGGAATCAACAATAGGGGCAGCATTTTTCTCCCAGACGTTGGCAGTCAATGACGCTACAGTGAAATTTGAGATTTGGGACACAGCAGGACAAGAGAGATACCATAGCTTGGCCCCCATGTACTATAGAGGCGCTGCTGCTGCTATCATTGTCTATGACATTACTAACTTGGATTCATTTACACGGGCGAAGAAATGGGTCCAAGAGCTTCAGAAACAAGGGAATCCTAACATGGTCGTGGCTTTTGCCGGTAACAAAGCTGACTTGGAAGATAAAAGGAAAGTGACAGCTGAAGAAGCACGTGTATATGCTGAGGAAAATGGTCTGTTTTTCATTGAGGCCTCTGCAAAAACTGCAGCCAATGTTAATGAAATATTTTATGAAATAGCCAAGAGGTTACCGAGGGCACAGCCAGCACAACCAGCAGGAATGGTTCTTGTTGATAGACCCGCCGAAGGATCCAGAGCTGCATCATGTTGTTCATAATTATTTATTCTGTTTGTTCCATCTTAGTTAAACTTGTTTTCTGTGTTTCTGTGTATACTATACGCACTTCGAATCTTTATGCGAAGCTCCAACAAGTTTATGGGCTGCAGATTACATAATGTAACTGGTTGGTCTGAATTATATCTCTTGGATGTTGACTTTTAGTCTGTTGATTGATCTGTTGTGTACATAGGGCGAAGTGCTTGAAAGTTGAAAGTCAAGCTCGTTTTAAAATTGTTCAAACATTCATTTTCTGAGTGCAGAAAATTCAATAAACACAAAATGAGGAGAACCTTCTAACRAG
TARGET=334,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03512 Ps_contig_mira-and-tgicl-ass_franssen_30228
SEQUENCE=TCGTAGCGAATTAAACGGCATTGTTTGTATTAATAATAAAGTGTAGAGAGAGAGAAAGAGAGATTTTGACAATCTTATCATATCTGAAATGGCTGCTTCGGGTATCGCTGGAGCAGGGCTTACCCTTCTCAACCGCTTCTCCTCTTCCGCTTCTTCTTCTTCAACTCGATGTCGTACTCGTTCTCTAACTATGGCTGTTTCCACCGACCAGAAACCCAAAACCACCGAAAAACTCACTCTCCGCAAGTCCGAAGAAGCTTTCGCCGCTGCAAAGGAATTGATGCCTGGAGGAGTTAACTCTCCCGTTCGTGCGTTCAAATCAGTTGGTGGACAACCCATTATCATTGACTCAGTTAAAGGCTCTCGGATGTGGGACATTGATGGCAACGAATATATTGACTACGTTGGTTCTTGGGGTCCTGCAATTATCGGTCACGCCGATGATCAGGTTCTTGCTGCTCTGGGGGAAACAATGAAGAAAGGAACCAGTTTTGGTGCACCTTGTCTATTGGAGAACACGTTAGCAGAGATGGTTATCGCTGCTGTTCCCAGCATTGAAATGGTGAGATTTGTGAATTCGGGAACGGAAGCGTGCATGGGTGCACTCCGTCTGGCGCGTGCTTTTACTGGGAAGGAGAAGATCATCAAGTTTGAGGGATGTTATCACGGACACGCGGATCCTTTTCTGGTTAAGGCTGGCAGTGGAGTTGCCACCCTAGGACTTCCGGATTCACCTGGTGTTCCTAAAGCTGCTACCAATGAAACGCTAACGGCCCCTTTCAATGATTTTGCTGCTGTTGAGAAGATCTTTGAGGACAACAAAGGAGAGATAGCTGTTGTTTTTCTTGAACCTGTTGTTGGAAATGCCGGTTTCATTACGCCTAAACCTGATTTTCTTAGTTCGTTGCGCAAAATTACTAAGGAAAATAATGCCCTTCTTGTGTTTGATGAAGTTATGACTGGATTTCGTTTGTCGTATGGAGGTGCTCAAGAATATTTTGGCATAACTCCTGATATAACAACTCTGGGGAAGATCATTGGAGGGGGTTTGCCGGTGGGTGCATATGGAGGTAGGAGGGATATTATGGAGACGGTGGCTCCGGCTGGACCTATGTACCAGGCCGGGACATTGAGCGGGAACCCTTTGGCCATGACTGCAGGCATACAGACTCTGAAGCGAATTAAAGAGGCAGGAACTTACGAATACTTGGACAACATTACAGGTGAGCTTGTTCAAGGAATTGTTGAAGCTGGGAAGAAGGCAGGGCACGCAATTTGTGGCGGGCATATAAATGGGATGTTTGGATTTTTCTTCACAGAAGGACCCGTGTACAATTTCACAGATGCAAAAAAGAGTGACACTGCTAAGTTTGCTAAGTTTTTCTGGGGTATGTTAGCAGAAGGTGTCTATTTGGCACCTTCACAGTTTGAGGCTGGGTTCACCAGCTTGGCACATACTTCTGATGACATCAAAAAAACTATAGCTGCTGCTGAGAGGGTCTTCAGGGAAATCTGAATGTTAAATTTTGTCTTACCGGTACTTGAGGTTTTGAAGCGTCATTTTTTTATACATTTGTAGGTTAAATTAGATCAATGCATTATTACTACGGCAGTTTTCATTATAATATCATTTTCACTTCTTGTATCTAGTCGGTGTATTGTTTAAAGCTATTAGGGAAGTCTCTTGAATTTGGAATTGTTGTAAGCATTATTCGAATCCATCGTTTAACTTATATAAGTCTCTTGAATTTGGAATTGTTGTAAGCATTTGAATCCATCGTTTAACTTATATAATTCGGAATTGTTATNAGCNTTTAG
TARGET=45,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4455
PRIMER_LEFT_PENALTY=0.381349
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=GTAGCGAATTAAACGGCATTGT
PRIMER_RIGHT_SEQUENCE=CGAGTACGACATCGAGTTGAAG
PRIMER_LEFT=2,22
PRIMER_RIGHT=179,22
PRIMER_LEFT_TM=60.381
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.4530
PRIMER_LEFT_1_PENALTY=0.381349
PRIMER_RIGHT_1_PENALTY=0.071684
PRIMER_LEFT_1_SEQUENCE=GTAGCGAATTAAACGGCATTGT
PRIMER_RIGHT_1_SEQUENCE=ACTTGCGGAGAGTGAGTTTTTC
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=247,22
PRIMER_LEFT_1_TM=60.381
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=0.4530
PRIMER_LEFT_2_PENALTY=0.381349
PRIMER_RIGHT_2_PENALTY=0.071684
PRIMER_LEFT_2_SEQUENCE=GTAGCGAATTAAACGGCATTGT
PRIMER_RIGHT_2_SEQUENCE=GACTTGCGGAGAGTGAGTTTTT
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=248,22
PRIMER_LEFT_2_TM=60.381
PRIMER_RIGHT_2_TM=59.928
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.5117
PRIMER_LEFT_3_PENALTY=0.381349
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=GTAGCGAATTAAACGGCATTGT
PRIMER_RIGHT_3_SEQUENCE=GAGTTGAAGAAGAAGAAGCGGA
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=166,22
PRIMER_LEFT_3_TM=60.381
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.5785
PRIMER_LEFT_4_PENALTY=0.381349
PRIMER_RIGHT_4_PENALTY=0.197140
PRIMER_LEFT_4_SEQUENCE=GTAGCGAATTAAACGGCATTGT
PRIMER_RIGHT_4_SEQUENCE=AACGAGTACGACATCGAGTTGA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=181,22
PRIMER_LEFT_4_TM=60.381
PRIMER_RIGHT_4_TM=59.803
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=180
=
PRIMER_SEQUENCE_ID=SSR_03513 Ps_contig_mira-and-tgicl-ass_franssen_30228
SEQUENCE=TCGTAGCGAATTAAACGGCATTGTTTGTATTAATAATAAAGTGTAGAGAGAGAGAAAGAGAGATTTTGACAATCTTATCATATCTGAAATGGCTGCTTCGGGTATCGCTGGAGCAGGGCTTACCCTTCTCAACCGCTTCTCCTCTTCCGCTTCTTCTTCTTCAACTCGATGTCGTACTCGTTCTCTAACTATGGCTGTTTCCACCGACCAGAAACCCAAAACCACCGAAAAACTCACTCTCCGCAAGTCCGAAGAAGCTTTCGCCGCTGCAAAGGAATTGATGCCTGGAGGAGTTAACTCTCCCGTTCGTGCGTTCAAATCAGTTGGTGGACAACCCATTATCATTGACTCAGTTAAAGGCTCTCGGATGTGGGACATTGATGGCAACGAATATATTGACTACGTTGGTTCTTGGGGTCCTGCAATTATCGGTCACGCCGATGATCAGGTTCTTGCTGCTCTGGGGGAAACAATGAAGAAAGGAACCAGTTTTGGTGCACCTTGTCTATTGGAGAACACGTTAGCAGAGATGGTTATCGCTGCTGTTCCCAGCATTGAAATGGTGAGATTTGTGAATTCGGGAACGGAAGCGTGCATGGGTGCACTCCGTCTGGCGCGTGCTTTTACTGGGAAGGAGAAGATCATCAAGTTTGAGGGATGTTATCACGGACACGCGGATCCTTTTCTGGTTAAGGCTGGCAGTGGAGTTGCCACCCTAGGACTTCCGGATTCACCTGGTGTTCCTAAAGCTGCTACCAATGAAACGCTAACGGCCCCTTTCAATGATTTTGCTGCTGTTGAGAAGATCTTTGAGGACAACAAAGGAGAGATAGCTGTTGTTTTTCTTGAACCTGTTGTTGGAAATGCCGGTTTCATTACGCCTAAACCTGATTTTCTTAGTTCGTTGCGCAAAATTACTAAGGAAAATAATGCCCTTCTTGTGTTTGATGAAGTTATGACTGGATTTCGTTTGTCGTATGGAGGTGCTCAAGAATATTTTGGCATAACTCCTGATATAACAACTCTGGGGAAGATCATTGGAGGGGGTTTGCCGGTGGGTGCATATGGAGGTAGGAGGGATATTATGGAGACGGTGGCTCCGGCTGGACCTATGTACCAGGCCGGGACATTGAGCGGGAACCCTTTGGCCATGACTGCAGGCATACAGACTCTGAAGCGAATTAAAGAGGCAGGAACTTACGAATACTTGGACAACATTACAGGTGAGCTTGTTCAAGGAATTGTTGAAGCTGGGAAGAAGGCAGGGCACGCAATTTGTGGCGGGCATATAAATGGGATGTTTGGATTTTTCTTCACAGAAGGACCCGTGTACAATTTCACAGATGCAAAAAAGAGTGACACTGCTAAGTTTGCTAAGTTTTTCTGGGGTATGTTAGCAGAAGGTGTCTATTTGGCACCTTCACAGTTTGAGGCTGGGTTCACCAGCTTGGCACATACTTCTGATGACATCAAAAAAACTATAGCTGCTGCTGAGAGGGTCTTCAGGGAAATCTGAATGTTAAATTTTGTCTTACCGGTACTTGAGGTTTTGAAGCGTCATTTTTTTATACATTTGTAGGTTAAATTAGATCAATGCATTATTACTACGGCAGTTTTCATTATAATATCATTTTCACTTCTTGTATCTAGTCGGTGTATTGTTTAAAGCTATTAGGGAAGTCTCTTGAATTTGGAATTGTTGTAAGCATTATTCGAATCCATCGTTTAACTTATATAAGTCTCTTGAATTTGGAATTGTTGTAAGCATTTGAATCCATCGTTTAACTTATATAATTCGGAATTGTTATNAGCNTTTAG
TARGET=150,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4530
PRIMER_LEFT_PENALTY=0.381349
PRIMER_RIGHT_PENALTY=0.071684
PRIMER_LEFT_SEQUENCE=GTAGCGAATTAAACGGCATTGT
PRIMER_RIGHT_SEQUENCE=ACTTGCGGAGAGTGAGTTTTTC
PRIMER_LEFT=2,22
PRIMER_RIGHT=247,22
PRIMER_LEFT_TM=60.381
PRIMER_RIGHT_TM=59.928
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.4530
PRIMER_LEFT_1_PENALTY=0.381349
PRIMER_RIGHT_1_PENALTY=0.071684
PRIMER_LEFT_1_SEQUENCE=GTAGCGAATTAAACGGCATTGT
PRIMER_RIGHT_1_SEQUENCE=GACTTGCGGAGAGTGAGTTTTT
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=60.381
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.6951
PRIMER_LEFT_2_PENALTY=0.676221
PRIMER_RIGHT_2_PENALTY=0.018835
PRIMER_LEFT_2_SEQUENCE=TCATATCTGAAATGGCTGCTTC
PRIMER_RIGHT_2_SEQUENCE=TCCGAGAGCCTTTAACTGAGTC
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=367,22
PRIMER_LEFT_2_TM=59.324
PRIMER_RIGHT_2_TM=60.019
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.7212
PRIMER_LEFT_3_PENALTY=0.381349
PRIMER_RIGHT_3_PENALTY=0.339815
PRIMER_LEFT_3_SEQUENCE=GTAGCGAATTAAACGGCATTGT
PRIMER_RIGHT_3_SEQUENCE=GAGAGTGAGTTTTTCGGTGGTT
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=240,22
PRIMER_LEFT_3_TM=60.381
PRIMER_RIGHT_3_TM=59.660
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=0.7479
PRIMER_LEFT_4_PENALTY=0.676221
PRIMER_RIGHT_4_PENALTY=0.071684
PRIMER_LEFT_4_SEQUENCE=TCATATCTGAAATGGCTGCTTC
PRIMER_RIGHT_4_SEQUENCE=GACTTGCGGAGAGTGAGTTTTT
PRIMER_LEFT_4=77,22
PRIMER_RIGHT_4=248,22
PRIMER_LEFT_4_TM=59.324
PRIMER_RIGHT_4_TM=59.928
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_03514 Ps_contig_mira-and-tgicl-ass_franssen_30239
SEQUENCE=GTCGGGGTAGAGTTTTGGTATAATTTTACCTATAATTAACAAAACCATACAAATCGATGAGGAAATGAGAATGGCACAATCCTTGATTGTCGCGACTCTTAGCCGGCAAGTGTTTAAAACGAACATTTCAACCGCACTCTTCCCTCACTCTTTCCCTCCTTTCTCTTCTACCTTTTTCAGATCTCGTTCATCTTCTTCGCTTTTCTTCAACCCAAAGATCCCTCTCTCCGCGTCTTTCTCTGCTGCAACCACCATGAGTCAAACTCCCGTTGCCGTTGATGCTGGCATGGACGCCGTTCAGCGCCGTCTCATGTTCGATGACGAATGCATTTTAGTGGATGAGAATGACCATGCGGTTGGTCATGAATCTAAATACAATTGCCACTTGTGGGAAAAGATTGAATCTGAAAATTGGCTGCATAGAGCTTTCAGTGTATTCCTGTTCAACTCAAAATATGAGTTGCTTCTTCAGCAACGATCTGCAACAAAGGTAACATTCCCTCTTGTGTGGACAAACACCTGCTGTAGCCATCCATTGTACCGAGAATCTGAGCTTATTGAAGAGAATGCCCTTGGAGTAAGAAATGCAGCTCAGAGGAAGCTTTTGGATGAGCTTGGTATTCCTGCTCAAGATGTACCGGTTGACCAGTTCACCCCACTGGGCCGCATACATTATAAGGCACCTTCTGATGGCAAATGGGGAGAGCATGAATTGGATTATTTGCTGTTTATTGTCCGGGATGTTAACGTTAATCCAAATCCTGATGAAGTGGCTGATATCAAATATGTGAACCGTGATCAGTTGAAGGAGCTGTTAAGGAAAGCCGACGCAGGGGAGGGAGGTTTGAAGCTGTCCCCTTGGTTCAGACTAGTTGTGGACAATTTCTTATTTAAATGGTGGGACCATCTTGAGCAAGGGACCCTTGGGGAGGTCATTGACATGAAGACAATTCACAAGTTGACATAAAATCAGAGATCTTGCAGCATAACATCAAAGGTTGATATTTTCCCAGCAAATAAAAAATGGAACTGTTTGAGGAGTTTTTGAAGTGTCGTTAAGTATGATTATCATCAGTGTTCCTTTCTGTTACTATTTCATAATATACGCAGAAACATTAACGCCTGAGTTTATAATTAGCAGAGTTTTGTTGCTAAATTTAAGATTTTGGGATGCGTTCAAATGTTCAATAATAAGCTATACTACTACTACTATTTAAAAATTGTTCTCTTGTTAAATTTGTTATGAATAAGGATGCTCGTGACTCGAGAATCTTTTTAAAGTTAATAAAGGGTATTACGAATATGGCC
TARGET=1199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7095
PRIMER_LEFT_PENALTY=0.049498
PRIMER_RIGHT_PENALTY=0.659953
PRIMER_LEFT_SEQUENCE=ATACGCAGAAACATTAACGCCT
PRIMER_RIGHT_SEQUENCE=GAGTCACGAGCATCCTTATTCA
PRIMER_LEFT=1102,22
PRIMER_RIGHT=1264,22
PRIMER_LEFT_TM=60.049
PRIMER_RIGHT_TM=59.340
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.8164
PRIMER_LEFT_1_PENALTY=0.156479
PRIMER_RIGHT_1_PENALTY=0.659953
PRIMER_LEFT_1_SEQUENCE=CAAAGGTTGATATTTTCCCAGC
PRIMER_RIGHT_1_SEQUENCE=GAGTCACGAGCATCCTTATTCA
PRIMER_LEFT_1=992,22
PRIMER_RIGHT_1=1264,22
PRIMER_LEFT_1_TM=59.844
PRIMER_RIGHT_1_TM=59.340
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.8318
PRIMER_LEFT_2_PENALTY=0.049498
PRIMER_RIGHT_2_PENALTY=0.782312
PRIMER_LEFT_2_SEQUENCE=ATACGCAGAAACATTAACGCCT
PRIMER_RIGHT_2_SEQUENCE=CTCGAGTCACGAGCATCCTTAT
PRIMER_LEFT_2=1102,22
PRIMER_RIGHT_2=1267,22
PRIMER_LEFT_2_TM=60.049
PRIMER_RIGHT_2_TM=60.782
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=0.9243
PRIMER_LEFT_3_PENALTY=0.264300
PRIMER_RIGHT_3_PENALTY=0.659953
PRIMER_LEFT_3_SEQUENCE=TCTTGCAGCATAACATCAAAGG
PRIMER_RIGHT_3_SEQUENCE=GAGTCACGAGCATCCTTATTCA
PRIMER_LEFT_3=976,22
PRIMER_RIGHT_3=1264,22
PRIMER_LEFT_3_TM=60.264
PRIMER_RIGHT_3_TM=59.340
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=0.9388
PRIMER_LEFT_4_PENALTY=0.156479
PRIMER_RIGHT_4_PENALTY=0.782312
PRIMER_LEFT_4_SEQUENCE=CAAAGGTTGATATTTTCCCAGC
PRIMER_RIGHT_4_SEQUENCE=CTCGAGTCACGAGCATCCTTAT
PRIMER_LEFT_4=992,22
PRIMER_RIGHT_4=1267,22
PRIMER_LEFT_4_TM=59.844
PRIMER_RIGHT_4_TM=60.782
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_03515 Ps_contig_mira-and-tgicl-ass_franssen_30244
SEQUENCE=TTGCAAAATAGGTAGGTTTTTGAGAAACAATAGTACAGTGAATACATTACATTGCAGTCTAAACAATTGTCATTTGCATAATTTAACAACATGGGTGAACAGTGTAAAACACGTGATTTACAACTCAACGCTAAAAAAGGAAGTACATGATATACACGGGAAAAAAAGAATACAAGCTATCTTATCTTATTGTTATTGAAAAAGCTACCAAAACTGATAAGTTAGATCCATTCCTTTGGGTTGATGCACGCATCTAAGAGTTTCCATTCTTCACTTCCTACTTCTGGTTTATGATCATATTCCCACCGAGAAGTCAGTGGGAGAGAAACAAGAATACTCTCTATTCTCCCTCCAGTTTTCAGTCCAAATGTTGTACCTCTGTCATATACCAAATTGAATTCAACATATCGTCCTCTTCTCAATTGTTGCCATGCTTTCTGACGTTCTGTGAAGGGCAAATCCTTTCTTTTCTCTATGATAGGTATGTATGCGGGAATAACAGAGTTTGCACATTCGGTAGCGAAGGAAAGGAGCATCTCCTGATCATAATCATTCAGATCATCGAAAAATATTCCTCCCAGCCCTCTCCTTTCATCTCGATGCTTGATATAGAAGTAATCGTCACACCATTTCTTGAATCGGGGGTAAAATGTAGGATCAAATTTGTCACAAGTCTGTTTTTGAATTGAGTGGAAGTGCTTGACATCCTCTTCAAAAATATAAGCAGGGGTTAAGTCGGTTCCTCCACCGAACCACCATTGTCTAGGTGCTCCAGGAGCGTCTTTAGGGGCATCAGTTTCAAAATAGCGATAATTGAAATGCAAGGTTGGGGCAAATGGGTTCTTTGGATGTAAAACAGAGCTGATTCCGGCAGCGAAAAAGGGAATAGGACCAGGCTTCTGATCCGCACTAGCACCACCTTTAGCAGCACGATAAGCATCGGGAGGCATTTGACCATAAACAACAGAAACATTAACACCAGCTTTCTCCCAAACATCACCATCTTGAAGAACCCTACTAATACCACCACCGCCACCCGGTCTAGACCAAACATCTTCTCTAAACTTTCCACCACCATCAGCAGCTTCAATCGCATTACAAACACTATCCTGCGCTTCTCTAATCATCTTCTCAAACCTAACCCTAACCGACGACACACCTCCGTCGCCATCGCCGCCTCGGAGAAACGTTTCCGGTCGTTCAGCTTCTGGAGTTTCCTTCTCTATTGAAACGGTTGCTTTTACTAGAACTCTTCGATGAGAGCGAGTGGATTTTGTTTGGAGTAGTGGATTAGGGTTTTGTTTGGTGAAGAGTTTTGTTGTTGGAAAATTTGGAGATGATTTTGAAGAGGTGAAGGTGAAGGGGATAACGGCGTATGACGGAGCTGTGACGGAACTTGTAGACGGCATTGTAACAGAATTCTGTTACGGTTAGATTTGAAGAAAAAAAAAGGTGATTGTGATTGGCGAGAGTTAGTTACAGATAAGAAGAAGAAGAGAGAAATGAAGCGAAGAAGGAAGAGGGCTTTCAAATTGAGTCACGAAGNNCCG
TARGET=1485,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0792
PRIMER_LEFT_PENALTY=0.045112
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=TGGTGAAGAGTTTTGTTGTTGG
PRIMER_RIGHT_SEQUENCE=TTCCTTCTTCGCTTCATTTCTC
PRIMER_LEFT=1303,22
PRIMER_RIGHT=1518,22
PRIMER_LEFT_TM=60.045
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=0.0792
PRIMER_LEFT_1_PENALTY=0.045112
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=TGGTGAAGAGTTTTGTTGTTGG
PRIMER_RIGHT_1_SEQUENCE=TCTTCCTTCTTCGCTTCATTTC
PRIMER_LEFT_1=1303,22
PRIMER_RIGHT_1=1520,22
PRIMER_LEFT_1_TM=60.045
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=218
PRIMER_PAIR_PENALTY_2=0.1209
PRIMER_LEFT_2_PENALTY=0.045112
PRIMER_RIGHT_2_PENALTY=0.075753
PRIMER_LEFT_2_SEQUENCE=TGGTGAAGAGTTTTGTTGTTGG
PRIMER_RIGHT_2_SEQUENCE=AATTTGAAAGCCCTCTTCCTTC
PRIMER_LEFT_2=1303,22
PRIMER_RIGHT_2=1533,22
PRIMER_LEFT_2_TM=60.045
PRIMER_RIGHT_2_TM=60.076
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.1228
PRIMER_LEFT_3_PENALTY=0.088662
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=GGATTAGGGTTTTGTTTGGTGA
PRIMER_RIGHT_3_SEQUENCE=TTCCTTCTTCGCTTCATTTCTC
PRIMER_LEFT_3=1287,22
PRIMER_RIGHT_3=1518,22
PRIMER_LEFT_3_TM=60.089
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=232
PRIMER_PAIR_PENALTY_4=0.1228
PRIMER_LEFT_4_PENALTY=0.088662
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=GGATTAGGGTTTTGTTTGGTGA
PRIMER_RIGHT_4_SEQUENCE=TCTTCCTTCTTCGCTTCATTTC
PRIMER_LEFT_4=1287,22
PRIMER_RIGHT_4=1520,22
PRIMER_LEFT_4_TM=60.089
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_03516 Ps_contig_mira-and-tgicl-ass_franssen_30260
SEQUENCE=TGCTTTACTTTGGGCAGCACAAGGATGAATCCATTGCTGCCTTACCCGTTGATGATTTAATAGAAAAGGCTGATGGATTTGCTGGGGTATTTCCTGAGCACAAGTATGAGATTGTGAAACGCTTGCAAGCTAGGAAACATATTTGTGGAATGACCGGTGATGGAGTGAATGATGCACCTGCTCTCAAGAAGGCCGACATTGGTATAGCTGTTGCAGATGCTACTGATGCAGCTCGAAGTGCATCTGATATTGTTTTAACCGAACCTGGCCTTAGTGTTATTATAAGTGCTGTTTTGACCAGCCGAGCTATCTTCCAGAGAATGAAGAATTATACAATTTATGCAGTCTCAATTACAATTCGTATTGTGCTTGGTTTCATGTTGCTGGCTCTGATATGGAAGTTCGACTTTCCGCCCTTTATGGTGCTTATTATTGCCATCCTAAATGATGGTACTATTATGACGATATCAAAAGATAGGGTGAAACCATCGCCTCTTCCAGATAGCTGGAAGCTATCCGAGATATTTACTACTGGGGTTGTGCTTGGTAGTTACTTGGCAATGATGACAGTTATATTCTTCTGGGCAGCATATAAAACAGATTTTTTCCCGAAAGTGTTTGGAGTTGCGACTCTTGAAAAAAACGCACACGATGATTTCCGAAAACTCGCCTCTGCAATCTATCTTCAAGTGAGCACTATTAGTCAGGCACTTATATTTGTTACTCGATCACGCGGTTGGTCATATGTCGAGCGTCCCGGTTTATTGCTGGTTGCTGCCTTTATTGTCGCTCAACTTATTGCAACTTTAATTGCGGTTTATGCTAGCTGGAGTTTCGCCGCAATTGAAGGAATAGGTTGGGGTTGGGCTGGTGTTATATGGCTATACAACATAATATTCTATATCCCGCTCGATTTCATTAAATTTTTCACTCGCTATGCTTTGAGCGGAAGGGCTTGGGATCTTGTCATTGAGCAAAGGATTGCTTTCACAAGGCAAAAGGATTTCGGCAAGGAACAAAGGGAGCTTCAATGGGCACATGCGCAAAGAACGCTGCATGGATTACAACCACCGGACACTAAGATGTTCACCGAGAGAACTCATGTCACCGAACTCAATCAAATGGCCGAAGAAGCTAAAAGGAGAGCTGAAATCGCAAGGTTGAGAGAGCTTCATACACTGAAGGGTCATGTAGAATCAGTTGTGAGATTGAAGGGACTTGACATAGACACCATTCAGCAAGCATATACAGTTTAAAGATGCTTATATATATTTGCATATTTTAATATTGTAAAGGTTACATCCTAATGTTGCTGAGATGAGAAGGATATATATAATGTAATGTAATGATTAAGCTCTCTTAAACTTTTGATAACTTTTTTATCTGCGGTTTGTTTTGAGCACTTAACAAGAGAAGTGCTTTTGTGACTTCTATTTTCTATCATCACTATATTCATGTCAAATTTAAAGTGATGNTTTTTTAGGGTATTCATATTAAAGAATTTTARNGTTAATTTTTTNANTCAATACTCAATTTATTCTAATAGT
TARGET=1334,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03517 Ps_contig_mira-and-tgicl-ass_franssen_30262
SEQUENCE=GTTCGGGTAACATTAGGGGCTACCATACCCATACCACACCACCAATATTCCCTTCTTTTCTTTCATTCAACAAAACCACATCCACATTCTCATTCACTTCACTTCACTTGCACACCATGTTACTGACAAAATGCTTCAGCAAGATCTTGTTCATTTTCTTCATAATTCCTTGCTCTATTATGTCCCTTCCTATACCAGATGCAGCAATGGTTCAAACTGTCGCAACAAATTCATCTTCACAAGGAACTATCCCTGCTTTCCCTGAACAAGCTGATATTGCAAGATGTCCTTTATCTCTGTCAGATGATCACTTCAACGGAATCAAAAACGCATGCAGTATCAAATCCAATAAACATGGTGGTGATTCTTATGATAAGCTTCAGCATAGTAGATGTTGTCCTGTGCTAGCTGCTTGGTTATATTCTGCATATTCTGCTACTGCTCTTGGAACTGGAAGGGTTTCTTCTGGTTTGGAACATGGACATGCTACTGCGTCTTATGATATGCCTTTGTTACCGGATGACTCTGAAACGTGTGAGAGTGATTTGGGAAGAGCTTTGAAAGTTAGAGGAATTGTGCTTTTTCAACCTAATGAGACTTGTGATGTGGTTTATTGTTATTGTGGTATTAGGCTTCATCCTTTAAGTTGTTCTGAATCGTTTAAGGTTACTCATAGTGGAAATCTTGTTGGAGATGAAAATGTGAAGAGATTGGAGAGAAATTGTTTGAGTAGTAGCAATGATGTTAATGGATTTCCTGGTCTAGGAGGTTGCTCTAAGTGCTTGCATAGCCTCTATTTGCTTAGAAAGAAGACTTCAAATTCAAGCAAAACAGGAGACAGAACAACAAAGATCCATAACAAAGATTGTGAACTAATGGGATTAACATGGCTTTTAGCTAAAAATAGAACAGCTTATATTCACACGGTTTCTTTGGTTCTTCGCGCCTTGATGTTGAGTCCTGATGGATCTGATCCACAATCATGCACTCTTAATAGTGATGGAATGCCTCTTGCTGTTGATTCATCTGAAATGTATGATCAATCTTCATCAACTAATCTCAAATCATCAATCTTTCTTTCTTTGTTGGTTCTATGTTTTGTACTAGTTATGCATTTCATTCAATTATCCACCTAAGTTGAGAACAAAAAGATTGATCTCTATCTTGGCATCTTGTAAATTTGAATATTAGCTTTTGTGTCAAGCTTCTAGGAGTGTAATTCTTATTAGTTATTAATTTAATTGAGTGGAGATGTTTTTTAAGYATTAATGAGAAGTTATTTAATTTAAGGTTT
TARGET=93,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03518 Ps_contig_mira-and-tgicl-ass_franssen_30262
SEQUENCE=GTTCGGGTAACATTAGGGGCTACCATACCCATACCACACCACCAATATTCCCTTCTTTTCTTTCATTCAACAAAACCACATCCACATTCTCATTCACTTCACTTCACTTGCACACCATGTTACTGACAAAATGCTTCAGCAAGATCTTGTTCATTTTCTTCATAATTCCTTGCTCTATTATGTCCCTTCCTATACCAGATGCAGCAATGGTTCAAACTGTCGCAACAAATTCATCTTCACAAGGAACTATCCCTGCTTTCCCTGAACAAGCTGATATTGCAAGATGTCCTTTATCTCTGTCAGATGATCACTTCAACGGAATCAAAAACGCATGCAGTATCAAATCCAATAAACATGGTGGTGATTCTTATGATAAGCTTCAGCATAGTAGATGTTGTCCTGTGCTAGCTGCTTGGTTATATTCTGCATATTCTGCTACTGCTCTTGGAACTGGAAGGGTTTCTTCTGGTTTGGAACATGGACATGCTACTGCGTCTTATGATATGCCTTTGTTACCGGATGACTCTGAAACGTGTGAGAGTGATTTGGGAAGAGCTTTGAAAGTTAGAGGAATTGTGCTTTTTCAACCTAATGAGACTTGTGATGTGGTTTATTGTTATTGTGGTATTAGGCTTCATCCTTTAAGTTGTTCTGAATCGTTTAAGGTTACTCATAGTGGAAATCTTGTTGGAGATGAAAATGTGAAGAGATTGGAGAGAAATTGTTTGAGTAGTAGCAATGATGTTAATGGATTTCCTGGTCTAGGAGGTTGCTCTAAGTGCTTGCATAGCCTCTATTTGCTTAGAAAGAAGACTTCAAATTCAAGCAAAACAGGAGACAGAACAACAAAGATCCATAACAAAGATTGTGAACTAATGGGATTAACATGGCTTTTAGCTAAAAATAGAACAGCTTATATTCACACGGTTTCTTTGGTTCTTCGCGCCTTGATGTTGAGTCCTGATGGATCTGATCCACAATCATGCACTCTTAATAGTGATGGAATGCCTCTTGCTGTTGATTCATCTGAAATGTATGATCAATCTTCATCAACTAATCTCAAATCATCAATCTTTCTTTCTTTGTTGGTTCTATGTTTTGTACTAGTTATGCATTTCATTCAATTATCCACCTAAGTTGAGAACAAAAAGATTGATCTCTATCTTGGCATCTTGTAAATTTGAATATTAGCTTTTGTGTCAAGCTTCTAGGAGTGTAATTCTTATTAGTTATTAATTTAATTGAGTGGAGATGTTTTTTAAGYATTAATGAGAAGTTATTTAATTTAAGGTTT
TARGET=1072,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03519 Ps_contig_mira-and-tgicl-ass_franssen_30263
SEQUENCE=CGTTGGTAAGTTCAAAAACCTTTAAGTCCCCCTTCCGTAGATCTATGTTCCTTAGATCTTCTATTCTGCTAGTCACTCTTATCTGCGCGCCTTTTCATCTGCTCCAGTTGTACCACTTGCTGTTGAAAGGGGTTGGACTGAGGCGTCTAGTCCAAATGTGAGAGGCCCACCTGCACTTCCTTACAAATTATCCCAATTAGACGAAAAGCTCAAAGCTGGTTATAAGTCAACAACTGCTGGGAAATTCACTGAAGCTCTCCGTACATTTGTTAATATTCTTCATACAATTCCTTTGATTGTCGTAGAGTCAAGGAGGGAAGTGGATGATGTAAAGGAATTGATAATCATAGTCAAAGAGTATGTTCTGGCTCTGCAGATGGAGCTGAAAAGAAGGGAAATGAAGGACAATCCAGTACGCCAGCAGGAGCTCGCAGCATATTTCACCCACTGCAATCTTCAGACACCTCACTTGAGGCTAGCTTTGTTGAATGCAATGACTGTCTGCTACAAAGACAAAGAACCTTGCCACTGCTGCTAACTTTGCCAGGAGACTACTTGAAACCAATCCTAGTATTGAAAACCAAGCTAAGACAGCAAGGCAAGTGCTAGCAGCTGCAGAAAGGAACATGACTGATGCCACAGAGTTGAACTATGATTTCCGAAACCCATTTGTGACTTGTGGGGCAACCTATGTGCCCATTTATCGCGGACAGAAGGATGTCTCTTGCCCATACTGTACTTCCCGTTTTGTACCAACCCATGAGGGGCAGCTATGCACTGTGTGTGAACTTTCAGTAGTAGGGGCAGATGCTTCTGGATTGCTCTGTTCTCCTGCCCAGATTCGTTGATTGAGCTTTTGGATGAGGAGCAGTTAATGATTAATTTTTGTTTCAGGTCATCTTTCAGCACATGATTAAAGTCGTATTGATCAATTCATATTATTTTCTGTAGAATCTAGAAATAGTCGAGAGTTATTGTTCTTGCGTGTAATTTTCTTTTGTTTTTTCTTTATTATTATTTTTTACTATGGTCTTACTTTCTTAATTACTTTTCGTCCCCTCCCTAAGTATAAGAGGGAAGGGGGAGGAAGGTTATGAATTATTTCGTGCAAGTGAAGTCTTCCACCNNTTATGTTAGAATTTATTTATTTTTTAGG
TARGET=1139,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03520 Ps_contig_mira-and-tgicl-ass_franssen_30265
SEQUENCE=TAATGGATCCTGTGGCCGATCTTGCCCCTTAGTCTAAAGCAAACGAATTATGCGACGTTAACCATGAATACCGTATTTAAACGGTTAAACCTTTTCTACTTTTGGTTAGTTTGGGTTTAAAGTTTGTATTCTGCTACATCTGGGTCTTGTATTCCCATTCGCAGGGCATGTTTGACATGTAAGACCAACATCACATGTACCACAAGTTTCTCCGATCTTGGAGCTGGATGAATAGGTGAAGAGACACAAAGCGATTAACACAAAGATATGAGTTTGTAAACTATGAATGCGAATGTCCCTAAGTAACCTCTTCATTGTTTTTTTGTTTGGTGAAGATAAAAAGTTTGTGACTGTTAAGAGAAAAACACTTAGTTAAGGAAGAGAAAAGCACTTGTTAAGAAAAGATTAAGGAACAAACGCACGTTACATTGACTAACACAACAACTGTCACACAACAACTACTCTCTTCTTTAGGTACATCACCTAACCCTGCTTAAATAAATAAAAAATATTAATAATGAGTATTTAAAAAACTTAAGATAAACTTGAATTAGAGAAT
TARGET=495,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03521 Ps_contig_mira-and-tgicl-ass_franssen_30268
SEQUENCE=AGGACTGCATTCACAATCATATTACACAGAGAGAGAGAGAATAAGAAAAACGAAAACACAAAAAGCGATTCGTCGTTTCGTCTGTGATCTCTTTCCATGTCGCCAACGAATCGCTTCTAACTGTTTCGATTCCGATCTCTGTGCTTACTCATAGTGGCATAGAATAGGATAACAGCCAACAAGAACTCTTCATTTTTCAACCGCAGCGCCATGGCAGCAGCACCCGCTAGAGCGCGTGCCGATTACGATTACCTCATCAAGCTTCTTCTTATCGGCGATAGTGGTGTGGGGAAGAGTTGTCTGCTTTTGAGATTTTCTGATGGTTCCTTCACAACCAGTTTTATCACCACCATTGGTATTGATTTCAAGATAAGAACCATTGAACTTGATGGCAAACGCATTAAGTTGCAAATCTGGGATACAGCTGGCCAGGAGAGATTCAGAACTATTACTACAGCTTATTACCGTGGAGCCATGGGTATCTTGCTGGTTTATGATGTTACTGATGAAGCATCTTTCAATAATATTAGGAATTGGATTCGCAATATTGAACAGCATGCTTCTGACAATGTAAACAAGATACTTGTGGGAAACAAGGCTGATATGGATGAAAGTAAAAGGGCTGTACCTACCTCCAAAGGTCAAGCTCTTGCTGATGAGTATGGTATCAAGTTCTTTGAAACTAGTGCAAAAACAAACATGAACGTGGAGGAAGTTTTCTTTTCAATAGCAAGAGACATCAAGCAAAGGCTTGCAGACACAGATTCTAGATCCGAGCCTCAGACTATCAAGATTAACCAACAAGATCCGGCAGCGAATGGCGGTCAAGCTGCAACAAAATCAGCTTGCTGCGGTTAGTGAAAAGAATCAATATCTGCAGTAAGACGATGCAGGTTTTACAGTAGTGCTCATTTGGGTGCTAATGTTATTCTGATGACAATATTCATGGGAGCATGTATTTGTCCCTCTCAATCTCCCAACTTCAGATGTCGTGTTTTAAAATTTATATTTTTAACTGTTTATTTTCCTCTTCATTTAGTTGGTGGAATTTGTGCTGTCTTGTTGAATTGCTTCAATTTCTTCTTTCATATGTAGTCTTTTACTCTAAGATATATTGATGGATAGATTATCACAACACTTCACTTGACAAATTGACAATGTATATCACGTACCAAAAT
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03522 Ps_contig_mira-and-tgicl-ass_franssen_30269
SEQUENCE=TGTGGGGAATCTGGTGTGTGCAGGAGCATTTATGAACAAGAAAAGTCTCGGCGTAACGCCACTGCATTCACATTCCTATTACACAGAGAGAGAGAAAAACGAAAACACAAAAAGCGATTCGTGGTTTCGTTCGTGATCCCTTTCCATGTCGCCAACGAATCGCTTCTAACTGTATCGATTCCGATCTCTGTGCTTACTGATAGTGGCATAGAATAGGATTACAGCGAAGAATAACTCTTCATTTTTCAACGTCAGCCATGGCAGCAGCACCCGCTAGAGCTCGTGCCGATTNNGATTACCTCATCAAGCTTCTTCTTATCGGCGATAGTGGTGTGGGGAAGAGTTGTCTGCTTTTGAGATTTTCTGATGGTTCCTTCACAACCAGTTTTATCACCACCATTGGTATTGATTTCAAGATAAGAACCATTGAACTTGATGGCAAACGCATTAAGTTGCAAATCTGGGATACAGCTGGCCAGGAGAGATTCAGAACTATTACTACAGCTTATTACCGTGGAGCCATGGGTATCTTGCTGGTTTATGATGTTACTGATGAAGCATCTTTCAATAATATTAGGAATTGGATTCGCAATATTGAACAACATGCTTCTGACAATGTAAACAAGATACTTGTGGGAAACAAGGCTGATATGGATGAAAGTAAAAGGGCTGTACCTACCTCCAAAGGTCAAGCTCTTGCTGATGAGTATGGTATCAAGTTCTTTGAAACTAGTGCAAAAACAAACATGAACGTGGAGGAAGTTTYCTTTTCAATAGCAAGAGACATCAAGCAAAGGCTTGCAGACACAGATTCTAGATCCGAGCCTCAGACTATCAAGATTAACCAACAAGATCCAGCAGCGAATGGGGGTCAAGCTGCAACAAAATCAGCTTGCTGCGGTTAGTGAAAAGAATCAATATCTGCAGTAAGAGGATGCAGGTTCTACAGTAGTGCTCATTTGGGTGCTAATGTTATTCCGATGACAATATTCGTGGGAGCATGTATTTGTCCCTCTCTATCTCCCAACCTCAGATGCCGTGTTTTAAAATTTATATTTTTAACTGTTTTCTTTCCTTTTCATTTAGTTGGTGTAATTTGTACTGACTTGTTGAATTACTTCGATTTCTTCTCTCATATGTAGTCTTTTACTCTAAGATATATTGACGGATATATTATCACAACACTTCACCTGCAAATTGCAATGTATATCAGTCCAAAATCATAAATGCATGGAAATAATTGCAGTTTTCTATCC
TARGET=85,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03523 Ps_contig_mira-and-tgicl-ass_franssen_30279
SEQUENCE=AGAAAACCGAAACTCACCTTCAGGAGTCAAAACGTCCATGAACGGAAACTGCTGGTGTAACAACCGCACATCATCGTCGTCAATACGCTACCGGCGGCGTAAAACTGCTCGCGCCTCAACCGTGCATCATCGCCGTCGATGCGTCTTCGCCAGAGTGGAACGGTTTGCGTAATGTCGTTACCAACACGTCGTCGGAGTGAAGTTGACGTCCACCTTGATCAGATCCGTAATCAAGCGATGTGTTCCACTTTCTACTTCTCTTTCTTCGTATCCTCCTTCGTCACCAGAAGAATCACACGAACCCGCGTGTTTGAGCCGTGTTGTTGTTGTTCGTCGTGGCGCGTCGTTGACCGTAACGGAGATGAAGGCGTTTGATTTTTGACCGCGATTTCGTCGTGACCGCACCGTTGYGAATTGGTGAAGGTTTGATGAAGGTGGAGATTACGGTTTCGGTTTACGG
TARGET=319,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03524 Ps_contig_mira-and-tgicl-ass_franssen_30282
SEQUENCE=AAATAAATAAATAGTGGGGTTTGTCGAGGACATCACAAATCGAGTCTCTCCCATTTCTCTGTCGAATTTGAGAAGCAATGGATTTGCTCACAACCTCTCCTTCTCTCTCACTCTCTCGCATTCCCACCCTTTTCTCAAAACAACCCATTTCTTCCTTCAACCCTAAAACCTTCACCCCTTCACCGTTTTCCCAATCTCGCACTACTCCAATCAGTTGCGTCCTCACAACCGCATCTCCAAAACCCTCAAATTTTGAATCCCATCTTCCAAAACCCTCAATCACTTTTCAATTATCTTCCCCTCAGACGCCGGAGAGGGCCATGCGAGGTGCTGAATCGGATACTATGGGGTTGTTGTTGAGGGAGAGGATTGTGTTCTTGGGAAGTGAAATTGATGACTTTGTTGCTGATGCTATTATGAGTCAGTTGTTGCTTTTGGATGCGCAAGATCCTAATAAAGATATTAAGCTTTTCATCAATTCCCCTGGTGGTTCTCTCAGTGCTACAATGGCTATCTATGACGCGGTACAGCTTGTGAGGGCTGATGTATCCACAATTGCAATGGGCATTGCAGCATCAACAGCCTCCATTATCCTCGGTGGTGGCACCAAAGGTAAGCGTCTAGCTATGCCCAATACGCGAATCATGATTACTCAACCACCCGGAGGTGCTAGTGGACAAGCTATAGATGTAGATATTCAAGCTACAGAAGTCATGCACGGCAAGAATAACGTTTCGAGAATTATATCATCCTTCACGGGTCGCTCGCTTGAACAAGTTCTGATAGATATTGATAGAGATAGATATCTGTCTCCCATTGAAGCAGTTGAATATGGAATTATTGACGGGGTAATCGACGGAGACAAGATTATTCCTCTTTTGCCAGTGCCAGAAAAGGTTGAAGTAATAACACTAGACACCACTGATCCGAGTTTCCTAACCCCAAATGTCCCCGACGATGAGATATACTAGCTTTATATGATCGAACGAATGCGATGTTGTAACAAAATGAAAGTTTCAGGATTATTTTGTAGATTGTTGTGACAATATTTGTCTTTCAACTATGATTCAGTTACTGTTAYTCTGGTTGTTCCTTYATTCTGTCTTAACTATTACAAAGGTTGAAATTTT
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03525 Ps_contig_mira-and-tgicl-ass_franssen_30283
SEQUENCE=GGTCTTAAAAAAGTACTAACATTTTGTATCATTAAAACAAGAAATAGNAAATCATTCTTTTATCATAGAAGTAAACCATAGTCGTAAGTCATATATCAAAGAGGATGTTTTATCCATAGCAAACCATATTATTAACCATACCATTCACGTGTCTACAAACTACAACCAATTATTTAATCTGCAAACCCCATAGAAGAAAGGAATAGATGTCTTGAATAGCAACACAAGACATCTATTCCTTGTGAAAATAGGAAGACTGAACCATACCACCTCAATCATGCACTGCAAGCACACAACGAAGACATTGCCCTTCATGCAAAAGATCGAAAGCCTTGTTGATCTCCAGAAGTGTCAAATTATGGGTAATGTACTCATCAACCTTGATTTCCTTCTTCAAGTACTTCTCTACAAGCCAAGGCACTTGTGACCTACTCTTAAAGCCACCAAAAGCTGTTCCTTTCCACACACGACCTGTCACCAACTGGAAAGGACGAGTTGATATTTCCTGCCCTGATGCTGCAACGCCCACAATAACTGATGTTCCCCAGCCCTTGTGGCAGCATTCCAAAGCAGATCTCATCACAGAGACATTTCCAATGCACTCAAAGCTATAATCAACTCCTCCATCTGTTAGATCACATATAACCTGCTGAATTGGTTTCTCGTGGTCTTTTGGATTAATGAACTCGGTGACTCCAAAGTTTTTAGCTGTATCATACTTGTTGCTATCAATATCTATGCCAATAATCCGTGATGCACCAGCACTTTTTGCACCCTCTGCAACAGCAAGACCAACAGTTCCAAGGCCGAAAATTGCAACAATTGACCCTGGCTCAACTTTTGCAGTGTTCCAGACAGCTCCAAGGCCAGTTGGAACACCACATCCAAGAAGACAAACTTTGTCCAAAGGAGCATCAGGATGAATCTTAGCTACACTAACATCATGAACAACAGTGTATTGACTAAAAGTAGAAGTTCCCATAAAATGATAAATCGGTTTTCCCTTAACAGAAAACCGAGACTTCCGATCCGCCATCATAACCCCAACACCAGTGGCAGCACGAACCTTCCCACAGAGGTTCGTCTTGCCGGATTTGCAAAACTTACATTCTCCACACTCAGCCTGGTAACACGGAATCACATGATCACCAGGCTTAACATCAGTCACACCTTCTCCAACACTTTCAACAATCCCTGCAGCTTCGTGGCCGAGAATGCAAGGGAAAAGACCTTCTGGATCTTTGCCGCCCAAAGTATATGCATCGGTGTGGCAGAGAGCGGTGAAGAGGATCTGGATTCGGACTTCGTTGGCTTGCGGCGGAGCAACCTCGACGTCTTCGATTGTTAAGGGTTTGTTGGGTTCCCAGGCCACCGCAGCTTTGCATGTAATGACTTGACCTTGAGTTGCCATTAGAGATGAGATTGAGAGTGAGGACTGAGGAGAGCCGAGTTGCCACCCCCGAGTNCTCANGNGNTATAAATAAATAAATAAATCTAACAATCCCCG
TARGET=1477,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03526 Ps_contig_mira-and-tgicl-ass_franssen_30284
SEQUENCE=GAATTGCATCAAAATATATCATGAATCGAAATTGAGACAAAACAAAACACATAGAAGACGTAGCTAATCGGTGTTTGGGGTCGTTCTCTTTCTCTTCTCTACACAAAAATGGCTTTCTCTATAATCACTACGCACTACTCTCCTCAATTTGTTCGCGTTCCCATCTCTGTTCCTCAACCTCGCACCTCTCTCTCTTTCCGATTTTCAACACCAGTTCTCAATCGCCGTCACAGTCCCAAACCTCTCTCAATCCGATCAGTTTCCGTTCCTGCCACCGCTGCTCCTGCATCTGAGGCCGTGGCACCTGCAATTTCGCTTTCAGATAATGCACTAAATCACTTGAATAAGATGAGGTCGGAACGAAATCAAGATTTGTGTTTAAGAATAGGCGTCAAACAGGGTGGGTGCTCTGGTATGTCATATGCAATGGATTTTGAGGACAGGGCTAATACAAGGCCAGATGACTCCATCATTGAATACAAAGGTTTTGTAATTGTTTGTGATCCTAAGAGCCTACTTTTCGTATTTGGGATGCAATTAGACTATAGTGATGCTCTGATTGGGGGAGGCTTCAATTTCAAGAATCCTAATGCGACACAGACCTGTGGATGTGGTAAATCCTTTAATGCAGAAATGTAAAGTCATGTTCATAGACATTAGTAACAATATTACAGTGATTGTTTCAAGCAATACTTTGGAATATTTATAGGATTGTATACTAACAATTAGTACATTATAGGAATTCTCGGAAAAAACTATTGAATTTGTCTGTTATTCCTATTTCTGTTTATGTTTGCTTTGCTGCATCAGTTTATTGTTAAGGTCTGTATAAACACTCATAGGATCTGTTGTATTTGCTATCCGAAATCTGTGTTATAATATGAATACTGGATCAGAAGAATTACTAAATAAAAAATCTCG
TARGET=186,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0548
PRIMER_LEFT_PENALTY=0.020747
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=GTTCCCATCTCTGTTCCTCAAC
PRIMER_RIGHT_SEQUENCE=TTCGTTCCGACCTCATCTTATT
PRIMER_LEFT=156,22
PRIMER_RIGHT=363,22
PRIMER_LEFT_TM=59.979
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.0548
PRIMER_LEFT_1_PENALTY=0.020747
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=GTTCCCATCTCTGTTCCTCAAC
PRIMER_RIGHT_1_SEQUENCE=TTTCGTTCCGACCTCATCTTAT
PRIMER_LEFT_1=156,22
PRIMER_RIGHT_1=364,22
PRIMER_LEFT_1_TM=59.979
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.0548
PRIMER_LEFT_2_PENALTY=0.020747
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=GTTCCCATCTCTGTTCCTCAAC
PRIMER_RIGHT_2_SEQUENCE=ATTTCGTTCCGACCTCATCTTA
PRIMER_LEFT_2=156,22
PRIMER_RIGHT_2=365,22
PRIMER_LEFT_2_TM=59.979
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.1389
PRIMER_LEFT_3_PENALTY=0.020747
PRIMER_RIGHT_3_PENALTY=0.118120
PRIMER_LEFT_3_SEQUENCE=GTTCCCATCTCTGTTCCTCAAC
PRIMER_RIGHT_3_SEQUENCE=TATGACATACCAGAGCACCCAC
PRIMER_LEFT_3=156,22
PRIMER_RIGHT_3=421,22
PRIMER_LEFT_3_TM=59.979
PRIMER_RIGHT_3_TM=59.882
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=0.2737
PRIMER_LEFT_4_PENALTY=0.239567
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=GGGGTCGTTCTCTTTCTCTTCT
PRIMER_RIGHT_4_SEQUENCE=TTCGTTCCGACCTCATCTTATT
PRIMER_LEFT_4=76,22
PRIMER_RIGHT_4=363,22
PRIMER_LEFT_4_TM=60.240
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_03527 Ps_contig_mira-and-tgicl-ass_franssen_30285
SEQUENCE=ATGTTGTGAAACACACAACTTGCACTTCTCATACTCTGCAGAGACGAGAGAGAAAGAAAGAGAGAGAGAGAGAGAGAAGCGCCTCTCACTCCTACTTCACTCTCACTCTTCTACTGCCAGACCACGTGAACCTACAATGGACTCGCGCGCACAGTATAATCCTCGCACCGTTGAAGGNAGTTTTAGGGATTCAAGGGTCGTCGAGCTGGTCYCATCAARGCTCTCACCACTGATGTT
TARGET=59,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03528 Ps_contig_mira-and-tgicl-ass_franssen_30286
SEQUENCE=AAGCAGTGTATCACGCAGAGTACGGGGGTTGTTGTGTTTGGTGTTATGTTGTGAAACACACAACTTGCACTTCTCATACTCTGCAGAGACGAGAGAGAAAGAAAGAGAGAGAGAGAAGCGCCTCTCACTCCTACTTCACTCTCACTCTTCTACTGCCAGACCACGTGAACCTACAATGGACTCGCGCGCACAGTATAATCCTCGCACCGTTGAAGAAGTTTTTAGGGATTTCAAGGGTCGTCGAGCTGGTCTCATCAAAGCTCTCACCACTGATGTTGAAGATTTCTATAACCAATGTGATCCTGATAAGGAGAATTTGTGCTTATACGGCTTTCCTAGTGAGCAGTGGGAAGTTAATTTACCTGCTGAAGAAGTTCCACCGGAGCTTCCCGAGCCTGTGCTCGGAATTAACTTTGCCAGGGATGGCATGCAAGAAAAAGATTGGCTGTCTTTAGTTGCTGTTCATAGCGATTCATGGTTACTTGCTATTGCCTACTATTTTGGAGCCAGATTCGGGTTTGACAAATCTGACAGGAAACGCCTTTTCAATATGATCAACGAACTGCCATCGATATTTGAAGTTGTTACTGGTACAGCAAAGAAACAAGTTAAGGAGAAGTCTTCAGTTTCAAACCACAGTGGAAGCAAATCCAAGTCCAGCTCAAAAGTGCGAGCTTCAGAACCCCAGGTTAAGCAGACAAAGGCGTTAGAACCTAAAGACGAGGAAGAAGAACTGGATGAGGAAGATGAAGATGAACACGGAGAGACCTTATGCGGTGCATGCGGTGAGCATTATGGAACAGACGAATTCTGGATTTGCTGCGACATCTGTGAGAAGTGGTTCCATGGGAAATGTGTGAAGATCACACCTGCCAGGGCTGAGCATATCAAGCAATACAAGTGTCCATCATGCAGTAACAACAAGAGAGCTCGCCCATAATGAGGCCAACAATGCTTGGATCGTTTTGTGATCGTGTTGTTGTTCTGTTGTTTCTCGTTCTAGATGTTGAAGTTATTGGATAGTTTCTGTTTGGCACATTTCGTAGGTGTTTGTTTTTTCTAGTTTATTATAGAATTGTGCTGCGCTGTGCTGTGCTGTGGTGGTTTTGTTGATGTTGTTGTCAGCTGCAATTCAGTTGATGACTTGTTGTGTTTGTTCAATTTATGTAGTTTATGAATTGTGGTGACTTTGGAAAGTGAAATCTTTTCCTCAATGTTAAAGAGGAAAACTAGGATAATGAAACAGAAAATTTTAAAAAAATTT
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2977
PRIMER_LEFT_PENALTY=0.293870
PRIMER_RIGHT_PENALTY=0.003820
PRIMER_LEFT_SEQUENCE=TTGTGAAACACACAACTTGCAC
PRIMER_RIGHT_SEQUENCE=GCCGTATAAGCACAAATTCTCC
PRIMER_LEFT=48,22
PRIMER_RIGHT=330,22
PRIMER_LEFT_TM=59.706
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.2977
PRIMER_LEFT_1_PENALTY=0.293870
PRIMER_RIGHT_1_PENALTY=0.003820
PRIMER_LEFT_1_SEQUENCE=TGTTGTGAAACACACAACTTGC
PRIMER_RIGHT_1_SEQUENCE=GCCGTATAAGCACAAATTCTCC
PRIMER_LEFT_1=46,22
PRIMER_RIGHT_1=330,22
PRIMER_LEFT_1_TM=59.706
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.3446
PRIMER_LEFT_2_PENALTY=0.293870
PRIMER_RIGHT_2_PENALTY=0.050763
PRIMER_LEFT_2_SEQUENCE=TTGTGAAACACACAACTTGCAC
PRIMER_RIGHT_2_SEQUENCE=CAGTGGTGAGAGCTTTGATGAG
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=271,22
PRIMER_LEFT_2_TM=59.706
PRIMER_RIGHT_2_TM=60.051
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.3446
PRIMER_LEFT_3_PENALTY=0.293870
PRIMER_RIGHT_3_PENALTY=0.050763
PRIMER_LEFT_3_SEQUENCE=TTGTGAAACACACAACTTGCAC
PRIMER_RIGHT_3_SEQUENCE=TGGTCTGGCAGTAGAAGAGTGA
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=162,22
PRIMER_LEFT_3_TM=59.706
PRIMER_RIGHT_3_TM=60.051
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=115
PRIMER_PAIR_PENALTY_4=0.3446
PRIMER_LEFT_4_PENALTY=0.293870
PRIMER_RIGHT_4_PENALTY=0.050763
PRIMER_LEFT_4_SEQUENCE=TGTTGTGAAACACACAACTTGC
PRIMER_RIGHT_4_SEQUENCE=CAGTGGTGAGAGCTTTGATGAG
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=271,22
PRIMER_LEFT_4_TM=59.706
PRIMER_RIGHT_4_TM=60.051
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_03529 Ps_contig_mira-and-tgicl-ass_franssen_30286
SEQUENCE=AAGCAGTGTATCACGCAGAGTACGGGGGTTGTTGTGTTTGGTGTTATGTTGTGAAACACACAACTTGCACTTCTCATACTCTGCAGAGACGAGAGAGAAAGAAAGAGAGAGAGAGAAGCGCCTCTCACTCCTACTTCACTCTCACTCTTCTACTGCCAGACCACGTGAACCTACAATGGACTCGCGCGCACAGTATAATCCTCGCACCGTTGAAGAAGTTTTTAGGGATTTCAAGGGTCGTCGAGCTGGTCTCATCAAAGCTCTCACCACTGATGTTGAAGATTTCTATAACCAATGTGATCCTGATAAGGAGAATTTGTGCTTATACGGCTTTCCTAGTGAGCAGTGGGAAGTTAATTTACCTGCTGAAGAAGTTCCACCGGAGCTTCCCGAGCCTGTGCTCGGAATTAACTTTGCCAGGGATGGCATGCAAGAAAAAGATTGGCTGTCTTTAGTTGCTGTTCATAGCGATTCATGGTTACTTGCTATTGCCTACTATTTTGGAGCCAGATTCGGGTTTGACAAATCTGACAGGAAACGCCTTTTCAATATGATCAACGAACTGCCATCGATATTTGAAGTTGTTACTGGTACAGCAAAGAAACAAGTTAAGGAGAAGTCTTCAGTTTCAAACCACAGTGGAAGCAAATCCAAGTCCAGCTCAAAAGTGCGAGCTTCAGAACCCCAGGTTAAGCAGACAAAGGCGTTAGAACCTAAAGACGAGGAAGAAGAACTGGATGAGGAAGATGAAGATGAACACGGAGAGACCTTATGCGGTGCATGCGGTGAGCATTATGGAACAGACGAATTCTGGATTTGCTGCGACATCTGTGAGAAGTGGTTCCATGGGAAATGTGTGAAGATCACACCTGCCAGGGCTGAGCATATCAAGCAATACAAGTGTCCATCATGCAGTAACAACAAGAGAGCTCGCCCATAATGAGGCCAACAATGCTTGGATCGTTTTGTGATCGTGTTGTTGTTCTGTTGTTTCTCGTTCTAGATGTTGAAGTTATTGGATAGTTTCTGTTTGGCACATTTCGTAGGTGTTTGTTTTTTCTAGTTTATTATAGAATTGTGCTGCGCTGTGCTGTGCTGTGGTGGTTTTGTTGATGTTGTTGTCAGCTGCAATTCAGTTGATGACTTGTTGTGTTTGTTCAATTTATGTAGTTTATGAATTGTGGTGACTTTGGAAAGTGAAATCTTTTCCTCAATGTTAAAGAGGAAAACTAGGATAATGAAACAGAAAATTTTAAAAAAATTT
TARGET=1085,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2178
PRIMER_LEFT_PENALTY=0.034537
PRIMER_RIGHT_PENALTY=0.183296
PRIMER_LEFT_SEQUENCE=TGTCCATCATGCAGTAACAACA
PRIMER_RIGHT_SEQUENCE=AATTGCAGCTGACAACAACATC
PRIMER_LEFT=901,22
PRIMER_RIGHT=1132,22
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=60.183
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.2178
PRIMER_LEFT_1_PENALTY=0.034537
PRIMER_RIGHT_1_PENALTY=0.183296
PRIMER_LEFT_1_SEQUENCE=TGTCCATCATGCAGTAACAACA
PRIMER_RIGHT_1_SEQUENCE=GAATTGCAGCTGACAACAACAT
PRIMER_LEFT_1=901,22
PRIMER_RIGHT_1=1133,22
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=60.183
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.2293
PRIMER_LEFT_2_PENALTY=0.034537
PRIMER_RIGHT_2_PENALTY=0.194782
PRIMER_LEFT_2_SEQUENCE=TGTCCATCATGCAGTAACAACA
PRIMER_RIGHT_2_SEQUENCE=CAGCTGACAACAACATCAACAA
PRIMER_LEFT_2=901,22
PRIMER_RIGHT_2=1127,22
PRIMER_LEFT_2_TM=60.035
PRIMER_RIGHT_2_TM=59.805
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=0.3488
PRIMER_LEFT_3_PENALTY=0.165541
PRIMER_RIGHT_3_PENALTY=0.183296
PRIMER_LEFT_3_SEQUENCE=TTCTGTTTGGCACATTTCGTAG
PRIMER_RIGHT_3_SEQUENCE=AATTGCAGCTGACAACAACATC
PRIMER_LEFT_3=1024,22
PRIMER_RIGHT_3=1132,22
PRIMER_LEFT_3_TM=60.166
PRIMER_RIGHT_3_TM=60.183
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=0.3488
PRIMER_LEFT_4_PENALTY=0.165541
PRIMER_RIGHT_4_PENALTY=0.183296
PRIMER_LEFT_4_SEQUENCE=TTCTGTTTGGCACATTTCGTAG
PRIMER_RIGHT_4_SEQUENCE=GAATTGCAGCTGACAACAACAT
PRIMER_LEFT_4=1024,22
PRIMER_RIGHT_4=1133,22
PRIMER_LEFT_4_TM=60.166
PRIMER_RIGHT_4_TM=60.183
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_03530 Ps_contig_mira-and-tgicl-ass_franssen_30288
SEQUENCE=GCAGTGGTATCAACGCAGAGTGTCATTTTTGTGAACACAAGGCGCAAAGTGGACTGGCTCACCGACAAGATGCGAAGCAGAGATCATACAGTCTCGGCCACTCACGGTGACATGGACCAGAACACTAGGGATATCATCATGCGTGAGTTTCGGTCTGGCTCTTCTCGTGTTCTCATTACCACTGACCTCCTGGCCCGTGGTATCGATGTGCAGCAGGTGTCTTTGGTCATCAACTATGATCTCCCAACTCAACCTGAAAACTATCTCCACCGCATTGGTCGTAGTGGACGATTTGGAAGAAAAGGTGTTTCTATTAACTTTGTGACATTGGATGATGCTAGAATGCTGGCTGATATTCAGAAATTCTACAATGTGACTATAGAGGAGCTGCCATCCAATGTTGCTGATTTGCTCTGATGAGTTCATTTTTGTTCTCTGTTAAGTGATCTTGGATTTTTCTCTTTTGGACAAGTTCTATCTTTTTTACTTTTGATTTAATTTTTTACTCATTAGAGATCAAACATGTGATCTGATGCTATTTGTATTGGGATTTTATCAATTTTGTTTGATTAATTAATTAGTTAAGAGAAGTTTGCATGTAATTTATATTCTTATCTCCTTTTGTACAATATCATATCCACTTTGATTTCTAACTATATGATTATTATG
TARGET=569,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03531 Ps_contig_mira-and-tgicl-ass_franssen_30304
SEQUENCE=GTTCTTAAAAACTCTTATRGANTRAGAATACATAATGGCATATATTTTGGCCAAAATACAAGAATGATTCAAAAAATAAATAAAGGTGGTAAAAATCAGGCAGTGATTTCCCCACCTTCCTATTCCTCAAGACTCAACTTTCACCAATCACAAAATCTACACCTACCCTACATTATGTTAGTAATCTAAAGAAATGATAAGAGCAATGGAGATGAGAAAAGAAAATGATTAAGGGGGAAAAGAGTCGGGGAGTGTGAGATTATCAACAGTAAATTTGCAATCAAAGCCACCATTACAAGATAATGAAGAAGATCATCATCTATCTGCATATAAAAAATCCTTCCAAATTCAGAATCCTTGTCTCTAATTAACTTAGCAACCATTACTCCGGCCGTTGTTGTCTGGGATCCTTAACATTGTTAAGTTTCATATATTCTGCAACTGAGAATAAGAGATCCTGATTACCGAGCCGGAAAGTGAGGTGGCATGCTTGCACACGAGGGCTTCGGTAGCCTGCTCCTCGGTCTCAAACTGAACCAGGCCTTGTTTCTTACTATTCATCTCAAAGACTTTGCTATTGACAATGGTTCCATGCTCCTCTAGAAGGGTCACAATCTCCTCCTCAGTGATGTCTTGTGGAAGTGTGGATAAATGGATCATCTTTGTTGGCGAGCAGCAATAACGGTAATTTTTAGCAGCATTTCGGTTGAAACGGTTGAGATTTGAGTTGCTGTAATCGTGTGTGTCAGCACCTTGGGTTATGTTTGGATGCTTTGAGAAGTTCACCTCGAGCCGCTTATCAAACAACATGGCTCCCTTCAGAAAGTGTACTGCCAGTTCAGCTTGGAAACCATCTCCCATCTGGATAAGTGCATGATCTGGTTTATTGCGTAGAAGTTTAATTCTCACGATGTTCCCGTAAAGGGAGAACAAGTTGAACAGTTTGTCCTCGTCGATTCTCTCAGGATTAAGATTTGAGACAAGAAGTGTACACCTATCATTTGTCCCGGTTATTCCTGGAGGCAAACCTCCCCCAAAAGCAGCTGCAATGGCGGCGGCATTGGGCATCTGCTGATATCCACCTGCTCTGGCTCCAGAACCCTGAACACCAGCATACATGTTTCCTGCATCACCATATCCAAGTTGTGAAGGTCTGCCTTTCTGTTCTGTAGGAAGACTAGGGTTTGTGAAGTCTCTTGATCGGTCATTATTGTAGTTCACTTGTAATTCATCAAGGTTTGAGAATTGAATGTCCAGCTGACAGCAACCATCATAAATATTGCGACCCTGAAGTGCGGTTCTCGCAGTAATAGCACTCTGTTGTGATTGGTACTGGATGAGAGCCTGAAAGCCAGCTGACTTCTGAAATGTCACAATCTTTTCCACAGATCCATGAGGAGAAAAAACTTGATACAGTACATCCACTGTTATGGGATAGAGCACGTGATGAATTGTAACTAAGAGAATTCGGTTTGGCTCATCTCCTCGTCCTTGGCTTTGATCTACTGTTGTTAGCTCTTGATGAGAGGAAAACTGGACATAAACATTCCTCCCCCTTATGCTAGGTTGAGTATTTGCATAATATTGTAAAGAACTTATTGCAGAAGGAACATCTTGCATTTGGATAAGTGCCTGATTTTTTGCACGCAACATCACAAGCTTGGTTATTACTCCAAAACGTTGAAATAGCTGAAGTAAATCATTTTCAGATATTTCGTGCCCCACGTTTCGAACATGAATGACTTTGGAAGGTTCCGTCATGGTAAACTAACCTGAGAGAGAGAGAAGTGAGGCACGTAGAATGTGGTGAATCTGTTAAGTTAACCCTATGTATGTTATGTTACGTTTGTTAATATGAATGAGATTCCCGAC
TARGET=1775,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03532 Ps_contig_mira-and-tgicl-ass_franssen_30317
SEQUENCE=CGGNGGNNTTTTAACTTTTTTACTTTTTTAATTTTTTGTTGTGTTGTCTAATATTTCTATGGCTTCTCATCTTTCTCTTCTTCGTGCTCCTCCTTTTTCAATTCCCCACTAAAAAACTTTCATTCCAAATATACTACCATAAAACCACTTAAACCCACCATCAATTTTCCTCCTTCTTCTTCTTTCTTTCATTGTTCTTCAATAGCATCACAAAGAGGATCTTGTTCATCACTGATTGCATGTTCCTCTTTAAATGGTAGAGGGCCAAATGATTCAGTGGATGATGGTGTTAAGAGCGTGGAACAACTTGTAGAAGAGAAACAACGTGCTGAACTGTCTGCTAGAATTGCCTCAGGAGAATTCACTGTAAAACAGGAATCTGGTTTACCTTCTATATTGAAGAAGAGCTTGTCAAATTTGGGAGTGTCCAATGAGATTTTGGAATTTTTATTTGGCCTGTATCCTAAAATTCCTGAGGCAAAAGGGTCGATTAAAGCCATTCGAAGTGAGGCTTTCTTTATTCCCTTGTATGAACTTTTCATCACATATGGTGGGATTTTCAGGCTTAATTTTGGTCCAAAGTCCTTTCTAATAGTATCGGATCCGGCTATTGCAAAACACATATTGAAAGACAATGCAAAGGCTTATTCTAAGGGTATCTTGGCTGAGATTCTTGATTTTGTAATGGGGAAAGGGCTTATCCCAGCTGACGGGGAAATATGGCGAGTTAGACGGCGTGCTATAGTTCCAGCATTGCATCTGAAGTTTTGTAGCAGCTATGATTGGTCCTTTTCGGACAAGCTACAGATAGGCTTTGCCAGAAGCTAGATACTGCTGCATCCGATGGAGAAGATGTTGAGATGGAGTCACTTTTCTCTCGATTGACGTTGGACGTCATTGGACAAAGCAGTGTTCAATTACGTA
TARGET=173,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2632
PRIMER_LEFT_PENALTY=0.226971
PRIMER_RIGHT_PENALTY=0.036271
PRIMER_LEFT_SEQUENCE=CTTTCTCTTCTTCGTGCTCCTC
PRIMER_RIGHT_SEQUENCE=TTGGCCCTCTACCATTTAAAGA
PRIMER_LEFT=70,22
PRIMER_RIGHT=267,22
PRIMER_LEFT_TM=59.773
PRIMER_RIGHT_TM=59.964
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=0.2632
PRIMER_LEFT_1_PENALTY=0.226971
PRIMER_RIGHT_1_PENALTY=0.036271
PRIMER_LEFT_1_SEQUENCE=TCTTTCTCTTCTTCGTGCTCCT
PRIMER_RIGHT_1_SEQUENCE=TTGGCCCTCTACCATTTAAAGA
PRIMER_LEFT_1=69,22
PRIMER_RIGHT_1=267,22
PRIMER_LEFT_1_TM=59.773
PRIMER_RIGHT_1_TM=59.964
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=199
PRIMER_PAIR_PENALTY_2=0.3516
PRIMER_LEFT_2_PENALTY=0.226971
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=TCTTTCTCTTCTTCGTGCTCCT
PRIMER_RIGHT_2_SEQUENCE=TACAGTGAATTCTCCTGAGGCA
PRIMER_LEFT_2=69,22
PRIMER_RIGHT_2=368,22
PRIMER_LEFT_2_TM=59.773
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.3516
PRIMER_LEFT_3_PENALTY=0.226971
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=CTTTCTCTTCTTCGTGCTCCTC
PRIMER_RIGHT_3_SEQUENCE=TACAGTGAATTCTCCTGAGGCA
PRIMER_LEFT_3=70,22
PRIMER_RIGHT_3=368,22
PRIMER_LEFT_3_TM=59.773
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.4793
PRIMER_LEFT_4_PENALTY=0.226971
PRIMER_RIGHT_4_PENALTY=0.252359
PRIMER_LEFT_4_SEQUENCE=TCTTTCTCTTCTTCGTGCTCCT
PRIMER_RIGHT_4_SEQUENCE=CTAGCAGACAGTTCAGCACGTT
PRIMER_LEFT_4=69,22
PRIMER_RIGHT_4=343,22
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=59.748
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_03533 Ps_contig_mira-and-tgicl-ass_franssen_30323
SEQUENCE=TCTCTGACTCATATACAACAACACTACTAGCAAGCATAGNAAATAAAACATAATCTTTCTTTCTTTTTGTCTTTCTTTATTCTTCTAGTGAATTTTCTCAACATAACAAAAATGGAGGAATGGTGTGAGTCAATGCCAAAGGTTGAACTGCATGCTCATCTCAATGGATCCATCAGAGTCTCTACACTCCTAAAACTTGCCAAGGCTTTGAATGAAACTCAAAAGGATGCTATAAATTTCTCTCAAATGGAACATGTCATCATGAAAAATGCTCTTTCATTGACAGAAGTATTCCATATGTTTGATCTAATTCACAATGTTATTACTGATCATAACATTGTTACAAGAATCACAAAAGAGGTCATTGAGGATTTTGCATCTGATAATGTTGTTTATTTGGAGTTGAGGACTACTCCAAAGAGAAACGATTCGATTGGAATGAGCAAGCGCTCGTACGTTGACGCTGTGATCGAAGGTCTAAAATCAGTTAGTTCAGTTGATGTCGATTTCATTCCAAAGACGGAAGATTCTAAAAGTTTTATCAATGATAAGTGCAATGGGAATTCGAGAAAAAGAATCATTGTTAGGCTTATCTTGAGCATCGACCGCAGAGAAACAACGGAAGCAGCTATGGAAACTGTTAAGCTTGCATTGGAAATGAGGCATTTCGGTGTAGTTGGAATTGATCTCTCTGGAAATCCGAAAACCGGTGATTGGACTACGTATTTACCGGCGCTGAAATTCGCGAGAGAACAAGGTCTTAATGTAACACTTCACTGCGGAGAGATACGAAATCCGAAAGAGATAAAGAACATGCTTGACCTTCATCCCCAAACGGATTGGACAATGCTTGTTTCTTCGAGGACGAGCATTGGAGAAGGATGAAATCTTCTAAAAATTCCGGTTGTAAAATTTTGTTTGTACATCAAACATAAGACATTCTCTGTTGCATCTATAGATGTTCACCTA
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03534 Ps_contig_mira-and-tgicl-ass_franssen_30328
SEQUENCE=GTTCGGGTAAGGTATTTGGCTAAAAGAAAAGAAAAAGAAAAGTCGACTTTTATGAAACCCAAAGGTTGAAGAAAGAGAAGAAGAAGAACTCGTTCATATTTTCTCACCCTTTGTGGCATTTCCACAAACACCTTAACTCCATCATCTATGGAGGCTCTTACTTCACTTCACCACCGTCACCAACCTCTATGTTCCTCTCTCTACCCTTCTCTAACATCATCAACTTCTTCTTCACTCTTTCTCTCACAATCTTCACCATCATCATCTTCCATTAGAGCTACATCGTCTTCTGATTCCCTTCAAAATCTTCAATACCCAGAACCCAAAACCCCTCAATTTTCCAACCCTCCTCTTCATGACGCAGAGCCCAAATCTCCTCAAATTTTCAACCCTAATCGTAAATTTCCAGGCGTTGACCCTCCTATTCAGGATCCAGAACCCAAATCTTCTCAATTTCTTAACCCTATTCGTAAATTTCCCTCTTTTGTGACGGTTACTGCAGCTGCATCGGCTTTTCTCTTTCTGGGTTATTGTCAAAATGGGTTTAACAAACCAATAACACCACTTTCCCCAGTTGTTTCTGTTGAGGAAAAAAACGGTTTTGAAGAATTTTCGGGGAGTAAACCATTTCAAGTTCAATCTGTTTTACATTTGAAGCTGAAAGAGAAAGTTCGTGTTGTGCATAGTTTCAAGAAAGTTAAAACTGATGATGATGAAGCATGGCAAGTGTTGAGAGGAGAGGTTTTTAGCTGCAGTGAGAATTTGGAGTTGATTAAGGTTGGATTTGAAGAGATACTGGAGAAAGACATGGATTGTAACAAGGTTCATCAAAATAGTGTTCTTGAGTATCTTGAAAGGGTTGATGAATGTAATAGCTTGTTAAAGGGTATCAAGGTTTGGATGGATAGATGTGAGTGGGAAGACGCGGATATAAATCGGCACCTCAGATTCTTCTGCAAGGTTGTTGATCAAATTAGGGTCTTGGAAGGAGACATGGTTAGTGCTTTGAAGTATTTTAAGCAGCTTGAACAATTGAACAAGAGTGAGTAACAGGAAATTCGAATGGAAGGTTTTCTCTTGGATGATTCGTTTCTGATAATCACCAAAGAAGATATTTGAGGTTTGATTTTTATTACTTTTACTACTTTTATTATATGATGTGTTAGTTTTAAAATAGTGGTTTATTAAGATATGATGAAGCACACGTATATAGTTAGGTGCTGAACATTTAAGTATTAGAAGCATAGCATGCATCTATGTTGCTGTATTATATGGTATGCAGCTTTTCATGTCTTTTGAGATTGCTTCCTGGTATGAGATGTTAATTTATGTGTANRATGGGAGGTAGGTTTTACCAAAGTTAGTAATTTAAAAGTTT
TARGET=76,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03535 Ps_contig_mira-and-tgicl-ass_franssen_30328
SEQUENCE=GTTCGGGTAAGGTATTTGGCTAAAAGAAAAGAAAAAGAAAAGTCGACTTTTATGAAACCCAAAGGTTGAAGAAAGAGAAGAAGAAGAACTCGTTCATATTTTCTCACCCTTTGTGGCATTTCCACAAACACCTTAACTCCATCATCTATGGAGGCTCTTACTTCACTTCACCACCGTCACCAACCTCTATGTTCCTCTCTCTACCCTTCTCTAACATCATCAACTTCTTCTTCACTCTTTCTCTCACAATCTTCACCATCATCATCTTCCATTAGAGCTACATCGTCTTCTGATTCCCTTCAAAATCTTCAATACCCAGAACCCAAAACCCCTCAATTTTCCAACCCTCCTCTTCATGACGCAGAGCCCAAATCTCCTCAAATTTTCAACCCTAATCGTAAATTTCCAGGCGTTGACCCTCCTATTCAGGATCCAGAACCCAAATCTTCTCAATTTCTTAACCCTATTCGTAAATTTCCCTCTTTTGTGACGGTTACTGCAGCTGCATCGGCTTTTCTCTTTCTGGGTTATTGTCAAAATGGGTTTAACAAACCAATAACACCACTTTCCCCAGTTGTTTCTGTTGAGGAAAAAAACGGTTTTGAAGAATTTTCGGGGAGTAAACCATTTCAAGTTCAATCTGTTTTACATTTGAAGCTGAAAGAGAAAGTTCGTGTTGTGCATAGTTTCAAGAAAGTTAAAACTGATGATGATGAAGCATGGCAAGTGTTGAGAGGAGAGGTTTTTAGCTGCAGTGAGAATTTGGAGTTGATTAAGGTTGGATTTGAAGAGATACTGGAGAAAGACATGGATTGTAACAAGGTTCATCAAAATAGTGTTCTTGAGTATCTTGAAAGGGTTGATGAATGTAATAGCTTGTTAAAGGGTATCAAGGTTTGGATGGATAGATGTGAGTGGGAAGACGCGGATATAAATCGGCACCTCAGATTCTTCTGCAAGGTTGTTGATCAAATTAGGGTCTTGGAAGGAGACATGGTTAGTGCTTTGAAGTATTTTAAGCAGCTTGAACAATTGAACAAGAGTGAGTAACAGGAAATTCGAATGGAAGGTTTTCTCTTGGATGATTCGTTTCTGATAATCACCAAAGAAGATATTTGAGGTTTGATTTTTATTACTTTTACTACTTTTATTATATGATGTGTTAGTTTTAAAATAGTGGTTTATTAAGATATGATGAAGCACACGTATATAGTTAGGTGCTGAACATTTAAGTATTAGAAGCATAGCATGCATCTATGTTGCTGTATTATATGGTATGCAGCTTTTCATGTCTTTTGAGATTGCTTCCTGGTATGAGATGTTAATTTATGTGTANRATGGGAGGTAGGTTTTACCAAAGTTAGTAATTTAAAAGTTT
TARGET=705,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03536 Ps_contig_mira-and-tgicl-ass_franssen_30334
SEQUENCE=GCATAACATTGATTATATAAATACTTGAAAAAGACAAATGTGTAATCTGTGTAAAACTTTGTTCATGTGGAAGTTTCAAAAGCTGTCAAGTTAAATAGAAAAACTAAACAGTTTTCCAACTTTAAAATTACACCTAATGTTTGCCGTATAAACAATGCTTTTAAGAAGCAATGACAATAACAATAATCCAAAGGAAGCCTTGGGGATACGCAAAATGACAAGTACATAAACAGTTCAGGCATTCTTTTCAATGATCAGCGAGCATAAAAGTACGACCTAACTGTAAAGATCATCATCATCAGCTCCGCCAGCAGATGTAAAAGGATCAGATGCACCTGTAGCAGCAGCACCACTGGAGCCAGAATCCGCAAACCTAAACTCAGTTCCAAACCCTCTGGATTGCTGCAATGTTTGGGCGAATGCCTGGTATTTGCGAATGTCGGCATCACTTACACTCCTTCGGGCATACTTCATTGATTCCTCAAAATGAGCTGCCTTAATTTCAGCTACTTCATCATCAACATCCTCTTCCATGGCCTCAGGGTTCTCACTTCTCTTTCTCTCTTTTTCAATGTCCTTTTCAATATTCTCTCTAATGGCATACTTGCATGCACGCTGGCAAATCTCAGTAATATCAGCACCACTAAAGCCTTGGGTGTACTTTGCAAGAGCTCTAATATCAACATCTTTTGAGATGGGGGATTTTCTCAAGCAAGCTTTAAATATCTGATGACGGGAATCTTCATCTGGGAGAGGGATATAAATCAACTGGTCTAGACGCCCTGGACGCAGAAGAGCCGGGTCAATAATGTCAGGTCGGTTAGTGGCGCCAATGATGAACACGGTTTTCTTTGCTGACATCCCATCCATTTCTGTTAGCAGCTGGTTCAAAACCCTGTCAGCAGCACCCCCAGCATCTCCTACACTACTACCTCTCTGAGTTGCAATGGAGTCAAGTTCGTCAAAGAATAGGACACATGGTGCAGAACCACGAGCCTTGTCAAAAATTTCCCTCACGTTTGCCTCACTTTCTCCAAACCACATCGTGAGCAGCTCAGGACCTTTGATACTGATGAAGTTTGCCTGGCATTCATTGGCAATAGCTTTTGCCAAAAGTGGTTTACCACAACCAGGAGGACCATAGAAAAGAACTCCCTTTGAAGGCGACATTCCAAACTTCTCAAACTTTTCAGGGTGTTCCACTGGATATTGAACAGTCTCCTGGAGTTCCCTCTTAACATTTTCAAGACCACCAATATCTTCCCAGCTGCAATTAGGCACTTCAACAACCGTTTCACGGAGAGCAGAAGGATTGCTTGATCCAAGAGCAGTAGCAAAATGCTCATTTGTCACTGCCATGGAGTTCAATATTTCAGCATCAATTGTCTCATCTTCCAAGTCAATCACATCCATTTTTTCTCTGATGCATTGAAGTGCTGCCTCAGTACACAATGCAGCTAAATCAGCACCAACATAACCATGTGTTTCCTTAGCAATTCTTTTCTAAATCAACATCTTCAGCAAGCTTCATATTCTTTGTATGAATTCGAAGAACCTCAAGACGCCCAATTTCATCTGGAACACCAATATCAATCTCCCTATCAAACCTTCCAAACCTCCTAAGTGCAGGGTCAATGCTATTGGGCCTATTTGTGGCTCCCATAACAATTACATGCGCGCGTGATTTAAGACCATCCATGAGTGTCAAGAGCTGGGAAACAATCCTCCTCTCAACTTCACCGTGAGTTTTCTCTCGCTTAGGAGCTATGGAATCTATCTCATCAATAAATATGATGGATGGAGCATTCTTCTCTGCTTCCTCAAATGCCTTCCTTAGATTGCTTTCACTTTCACCAGCGAGTTTTGACATTATCTCAGGGCCATTAATGCAGAAAAAGAAGGCACCT
TARGET=289,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0575
PRIMER_LEFT_PENALTY=0.036271
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=CAATAATCCAAAGGAAGCCTTG
PRIMER_RIGHT_SEQUENCE=AAGTATGCCCGAAGGAGTGTAA
PRIMER_LEFT=180,22
PRIMER_RIGHT=470,22
PRIMER_LEFT_TM=59.964
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.1466
PRIMER_LEFT_1_PENALTY=0.125411
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=CAATGATCAGCGAGCATAAAAG
PRIMER_RIGHT_1_SEQUENCE=AAGTATGCCCGAAGGAGTGTAA
PRIMER_LEFT_1=248,22
PRIMER_RIGHT_1=470,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.1654
PRIMER_LEFT_2_PENALTY=0.036271
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=CAATAATCCAAAGGAAGCCTTG
PRIMER_RIGHT_2_SEQUENCE=TGAGTTTAGGTTTGCGGATTCT
PRIMER_LEFT_2=180,22
PRIMER_RIGHT_2=381,22
PRIMER_LEFT_2_TM=59.964
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.1654
PRIMER_LEFT_3_PENALTY=0.036271
PRIMER_RIGHT_3_PENALTY=0.129103
PRIMER_LEFT_3_SEQUENCE=CAATAATCCAAAGGAAGCCTTG
PRIMER_RIGHT_3_SEQUENCE=GAGTTTAGGTTTGCGGATTCTG
PRIMER_LEFT_3=180,22
PRIMER_RIGHT_3=380,22
PRIMER_LEFT_3_TM=59.964
PRIMER_RIGHT_3_TM=60.129
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=0.1654
PRIMER_LEFT_4_PENALTY=0.036271
PRIMER_RIGHT_4_PENALTY=0.129103
PRIMER_LEFT_4_SEQUENCE=CAATAATCCAAAGGAAGCCTTG
PRIMER_RIGHT_4_SEQUENCE=CTGAGTTTAGGTTTGCGGATTC
PRIMER_LEFT_4=180,22
PRIMER_RIGHT_4=382,22
PRIMER_LEFT_4_TM=59.964
PRIMER_RIGHT_4_TM=60.129
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_03537 Ps_contig_mira-and-tgicl-ass_franssen_30339
SEQUENCE=TACTTACAATATTTATACTTATATTTCATAAATAGATTCAGCTACAAATATGAACACTGATAAACATAACAAGTGTCTTCTCTTCTCCCGGCCAAGCAAGAAAAAAACCAGCTGTGAACAAGGTAACAAGAGATTTTACAATAATATACTTAAAGTAGTAGCACTGCAATTGTCAAAAATTCCATTGCAGTAACCACTTTTCTAAGAAACTAAAATCTAGTTACATGCATGACATGAGAATCTTCACTAGAAAATTTATAAAAAAATTACATATAAGGACACTAAACTTCACAGAGAATCCCAATCAATCTCATAAGAGGGGTACTTCAGCAAATTAAAATTTTCCGAATCACCTTCCCACTGTGGTTCACCAACATCACCAAAAGTAAGAACTGAAAGTGGTGAAGAACCTTCACTCTCGGTCTCGGTCATCACCGGAGAAATCGAAGCTTCAACCTTACAACACCCTTCTGAAACTGAACCAGAACCCTTCTCCAAATCATCAACAGACTTCTCCACCGTCTCCGGCGAAGGTACCACCTTCGACGCAGCTTTGGACCGAGCCGCTGGTGTTTTTTAGGCTTCTCTGACTTCCCCTGTTTCTGCATCTCAGCTAAACCTTCACAAATAGCTTGAAGTTTAGCATCAACCGAGGAAGGAAGAGGTTTATACTCACCAAACTCACCGACACCAATACATGAACCTTGGTGTTTGAGATTCGGGAAATTAAGCCTAGCAAAGTCACCACGAAGCTTATAAGCAGCTTTGTCATAAGCCAAAGCAGCTTCTTCTGCTGTATCAAAGGTACCAAGCCAAAGCCTGGTTCGATTCTTAGGGAGTCTGATCTCAGCAACCCATTTTCCCCAGTGCCTTTGCCTCACCCCTCTGTATAGCTTCGTGGGTTTTGAAGGAGTATTACCCACGTGCTTCATAGGGATTGGCTTCGGACTGAGGAAGCTAAGAGTGTTACTGAGACGTTGCTGTTGTTGATGTTGTTGCAAGGTAAAGTTTTGGTGTTGGATTTGTGCTTGGATCTGGTTGATTTGAGATGGGGTCAAATGGTTAAGACCAAGAAGAGAAGAAGAAGATGGTTGTTGAACAAAGCCTATGAAGTTCTGGCTATTCGATAACCCGTTGGTAAATCCATGGGTCATCGATGTTGAACAACCATCATTGTAGAAAGAATGGAAAGAAGAAGAAGAAGAAGGTGATGGTGTTGAATTCGGAGAAGGACTTTTGATAAAAGGTTCAAGAACTTCCATTAATTCACCTCTAAAAGGATCTGAATATGGATCTAAGCGAAGTTGTATGTTATTGTATAAATTCATCATAGCTGCCATATTTCCTGCGAAAACTTATACCCGAATCTTTCTAAAATTGAAGAACAGGTTAATTAAAGGAAAAAAATTCAACAAGGGATATAGAAGGGATTTTAAATAGACAATCCTTTCAAACCTTGAATTAAGAAGAAAAAAAATAGCCTTGAAGGGATCAAAAACAACCCTCTTGTGGCTAATTTCAAAGAACTTTACAGTTAAAATAAAAGCTGGATCTCGATTCTGAAAACACCTGGCTTTCGAAAAATTCTTCTAAGTTTGTTACTTTTTAATTTGATTAAAAGACAAACCNAANAATAATGAAGACAGATAATAACAGAAGG
TARGET=1077,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1330
PRIMER_LEFT_PENALTY=0.057411
PRIMER_RIGHT_PENALTY=0.075553
PRIMER_LEFT_SEQUENCE=GTTGTTGATGTTGTTGCAAGGT
PRIMER_RIGHT_SEQUENCE=GATTTACCAACGGGTTATCGAA
PRIMER_LEFT=982,22
PRIMER_RIGHT=1143,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=60.076
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=162
PRIMER_PAIR_PENALTY_1=0.1430
PRIMER_LEFT_1_PENALTY=0.067436
PRIMER_RIGHT_1_PENALTY=0.075553
PRIMER_LEFT_1_SEQUENCE=TGGATCTGGTTGATTTGAGATG
PRIMER_RIGHT_1_SEQUENCE=GATTTACCAACGGGTTATCGAA
PRIMER_LEFT_1=1029,22
PRIMER_RIGHT_1=1143,22
PRIMER_LEFT_1_TM=59.933
PRIMER_RIGHT_1_TM=60.076
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=0.2041
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=0.075553
PRIMER_LEFT_2_SEQUENCE=TTCGTGGGTTTTGAAGGAGTAT
PRIMER_RIGHT_2_SEQUENCE=GATTTACCAACGGGTTATCGAA
PRIMER_LEFT_2=894,22
PRIMER_RIGHT_2=1143,22
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=60.076
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.2041
PRIMER_LEFT_3_PENALTY=0.128591
PRIMER_RIGHT_3_PENALTY=0.075553
PRIMER_LEFT_3_SEQUENCE=TCGTGGGTTTTGAAGGAGTATT
PRIMER_RIGHT_3_SEQUENCE=GATTTACCAACGGGTTATCGAA
PRIMER_LEFT_3=895,22
PRIMER_RIGHT_3=1143,22
PRIMER_LEFT_3_TM=59.871
PRIMER_RIGHT_3_TM=60.076
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.2139
PRIMER_LEFT_4_PENALTY=0.057411
PRIMER_RIGHT_4_PENALTY=0.156479
PRIMER_LEFT_4_SEQUENCE=GTTGTTGATGTTGTTGCAAGGT
PRIMER_RIGHT_4_SEQUENCE=CATGGATTTACCAACGGGTTAT
PRIMER_LEFT_4=982,22
PRIMER_RIGHT_4=1147,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=59.844
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_03538 Ps_contig_mira-and-tgicl-ass_franssen_30339
SEQUENCE=TACTTACAATATTTATACTTATATTTCATAAATAGATTCAGCTACAAATATGAACACTGATAAACATAACAAGTGTCTTCTCTTCTCCCGGCCAAGCAAGAAAAAAACCAGCTGTGAACAAGGTAACAAGAGATTTTACAATAATATACTTAAAGTAGTAGCACTGCAATTGTCAAAAATTCCATTGCAGTAACCACTTTTCTAAGAAACTAAAATCTAGTTACATGCATGACATGAGAATCTTCACTAGAAAATTTATAAAAAAATTACATATAAGGACACTAAACTTCACAGAGAATCCCAATCAATCTCATAAGAGGGGTACTTCAGCAAATTAAAATTTTCCGAATCACCTTCCCACTGTGGTTCACCAACATCACCAAAAGTAAGAACTGAAAGTGGTGAAGAACCTTCACTCTCGGTCTCGGTCATCACCGGAGAAATCGAAGCTTCAACCTTACAACACCCTTCTGAAACTGAACCAGAACCCTTCTCCAAATCATCAACAGACTTCTCCACCGTCTCCGGCGAAGGTACCACCTTCGACGCAGCTTTGGACCGAGCCGCTGGTGTTTTTTAGGCTTCTCTGACTTCCCCTGTTTCTGCATCTCAGCTAAACCTTCACAAATAGCTTGAAGTTTAGCATCAACCGAGGAAGGAAGAGGTTTATACTCACCAAACTCACCGACACCAATACATGAACCTTGGTGTTTGAGATTCGGGAAATTAAGCCTAGCAAAGTCACCACGAAGCTTATAAGCAGCTTTGTCATAAGCCAAAGCAGCTTCTTCTGCTGTATCAAAGGTACCAAGCCAAAGCCTGGTTCGATTCTTAGGGAGTCTGATCTCAGCAACCCATTTTCCCCAGTGCCTTTGCCTCACCCCTCTGTATAGCTTCGTGGGTTTTGAAGGAGTATTACCCACGTGCTTCATAGGGATTGGCTTCGGACTGAGGAAGCTAAGAGTGTTACTGAGACGTTGCTGTTGTTGATGTTGTTGCAAGGTAAAGTTTTGGTGTTGGATTTGTGCTTGGATCTGGTTGATTTGAGATGGGGTCAAATGGTTAAGACCAAGAAGAGAAGAAGAAGATGGTTGTTGAACAAAGCCTATGAAGTTCTGGCTATTCGATAACCCGTTGGTAAATCCATGGGTCATCGATGTTGAACAACCATCATTGTAGAAAGAATGGAAAGAAGAAGAAGAAGAAGGTGATGGTGTTGAATTCGGAGAAGGACTTTTGATAAAAGGTTCAAGAACTTCCATTAATTCACCTCTAAAAGGATCTGAATATGGATCTAAGCGAAGTTGTATGTTATTGTATAAATTCATCATAGCTGCCATATTTCCTGCGAAAACTTATACCCGAATCTTTCTAAAATTGAAGAACAGGTTAATTAAAGGAAAAAAATTCAACAAGGGATATAGAAGGGATTTTAAATAGACAATCCTTTCAAACCTTGAATTAAGAAGAAAAAAAATAGCCTTGAAGGGATCAAAAACAACCCTCTTGTGGCTAATTTCAAAGAACTTTACAGTTAAAATAAAAGCTGGATCTCGATTCTGAAAACACCTGGCTTTCGAAAAATTCTTCTAAGTTTGTTACTTTTTAATTTGATTAAAAGACAAACCNAANAATAATGAAGACAGATAATAACAGAAGG
TARGET=1190,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1851
PRIMER_LEFT_PENALTY=0.075553
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=TTCGATAACCCGTTGGTAAATC
PRIMER_RIGHT_SEQUENCE=AAGTCCTTCTCCGAATTCAACA
PRIMER_LEFT=1122,22
PRIMER_RIGHT=1235,22
PRIMER_LEFT_TM=60.076
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=0.2535
PRIMER_LEFT_1_PENALTY=0.143968
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=TTCGGACTGAGGAAGCTAAGAG
PRIMER_RIGHT_1_SEQUENCE=AAGTCCTTCTCCGAATTCAACA
PRIMER_LEFT_1=942,22
PRIMER_RIGHT_1=1235,22
PRIMER_LEFT_1_TM=60.144
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=0.2535
PRIMER_LEFT_2_PENALTY=0.143968
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=CTTCGGACTGAGGAAGCTAAGA
PRIMER_RIGHT_2_SEQUENCE=AAGTCCTTCTCCGAATTCAACA
PRIMER_LEFT_2=941,22
PRIMER_RIGHT_2=1235,22
PRIMER_LEFT_2_TM=60.144
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.3294
PRIMER_LEFT_3_PENALTY=0.219844
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=GCTATTCGATAACCCGTTGGTA
PRIMER_RIGHT_3_SEQUENCE=AAGTCCTTCTCCGAATTCAACA
PRIMER_LEFT_3=1118,22
PRIMER_RIGHT_3=1235,22
PRIMER_LEFT_3_TM=60.220
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=0.3684
PRIMER_LEFT_4_PENALTY=0.258908
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=GGGTCAAATGGTTAAGACCAAG
PRIMER_RIGHT_4_SEQUENCE=AAGTCCTTCTCCGAATTCAACA
PRIMER_LEFT_4=1051,22
PRIMER_RIGHT_4=1235,22
PRIMER_LEFT_4_TM=59.741
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_03539 Ps_contig_mira-and-tgicl-ass_franssen_30340
SEQUENCE=CAATGCGACCTGCAAGAGTACGTCGGGGATCCTCTTCAAAACTCTAAACCCTTACTACTACTTAACAAAATGGCATTTTCTTCTTCTTCATCATCTTCTAAAGTTTCAATAAACTTTATTTGCTTTGTTTTGTTGATGTTTTTTCTTATAGGAAACTCTTCAGCTCAACTCTCTGAAAATTTCTATGCTAAAAAGTGTCCCAACGTTTTCAATGTTGTAAAGTCTATCGTTCACTCTGTTGTGGCTAATGAACCTCGCATGGGAGGATCTCTACTTCGACTCTTCTTTCATGACTGCTTTGTTAATGGTTGTGATGGATCAGTGCTACTTGATGACACATCCTCCATGAAAGGAGAGAAAACTGCACCTCCCAACAAAGATTCTTTGAGAGGTTTTGAAGTAATTGATGCCATTAAATCTAAGGTGGAAGCAGCATGTCCTGGTGTTGTCTCATGTGCTGATATTGTAGCCATTACAGCACGTGACTCAGTTGTAAATCTTGGAGGACCTTATTGGAAGGTTAAACTTGGAAGAAGGGATTCAAAAACAGCGAGTTTCAACGATGCAAGCAATGGTGCTATTCCACCTCCCTTTTCTACTCTTGCCAACCTTATCAATAGGTTTAAAGCTCAAGGACTCTCTACTAAGGACATGGTAGCCTTATCTGGAGCTCACACTATTGGTAAGGCAAGATGTGTTGTTTATAGAGATCACATTTACAATGATACAGACATTGATAGTTTATTTGCCAAGTCAAGGCAAAGGAACTGTCCAAGGAAAAGTGGTTCAATAAAGGACAACAATGTTGCATTTCTTGATTACAAAACTCCAAACCATTTTGACAATGTCTACTACAATAATCTCATCAACAAAAAGGGACTCCTTCATTCTGATCAAGTGCTTTTCAATGGAGGTTCTACTGATTCATTGGTTAAAACTTATAGTAACAACCCAAAAGCCTTTGAATCCGATTTTGTTACTGCTATAATTAAGATGGGGAATATTAAGCCTCTTACCGGATCAAAAGGAGAGATTAGGAAGCAATGCAGAAGAGCCAATTGAATTGAAGGAGATATCTGAAGTATCGACATTAAAGATTGAAGGCGTGTCAGACATTAACTGACACTAATTTATTATTGATGTTTACGTATTAGTATCTACGATGGTGTTATGGTTTTTTTAAAGTACACATATTTACTCCAAAAGTGGTGGTAAATTTATTTGTTTTAGTGTTTTGATGTGGTTTGGAAAATTATTTTGTTGGTATGTTTAATTTTATATTTTGCTCAAGTTAGTGTAAGTTAAATTGCAAAGCATCTTGTGACAAAAACTGTCACATTAAGAATGTGTGTATTGCAGTAATCATATATTAAAATGAATACATATTTGTTACCATAANCNTTATCT
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1079
PRIMER_LEFT_PENALTY=1.041548
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=GGGGATCCTCTTCAAAACTCTAA
PRIMER_RIGHT_SEQUENCE=ACGTTGGGACACTTTTTAGCAT
PRIMER_LEFT=24,23
PRIMER_RIGHT=205,22
PRIMER_LEFT_TM=59.958
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=1.2387
PRIMER_LEFT_1_PENALTY=1.041548
PRIMER_RIGHT_1_PENALTY=0.197140
PRIMER_LEFT_1_SEQUENCE=GGGGATCCTCTTCAAAACTCTAA
PRIMER_RIGHT_1_SEQUENCE=AGCCACAACAGAGTGAACGATA
PRIMER_LEFT_1=24,23
PRIMER_RIGHT_1=245,22
PRIMER_LEFT_1_TM=59.958
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=1.2387
PRIMER_LEFT_2_PENALTY=1.041548
PRIMER_RIGHT_2_PENALTY=0.197140
PRIMER_LEFT_2_SEQUENCE=GGGGATCCTCTTCAAAACTCTAA
PRIMER_RIGHT_2_SEQUENCE=TAGCCACAACAGAGTGAACGAT
PRIMER_LEFT_2=24,23
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=59.958
PRIMER_RIGHT_2_TM=59.803
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=1.2387
PRIMER_LEFT_3_PENALTY=1.041548
PRIMER_RIGHT_3_PENALTY=0.197140
PRIMER_LEFT_3_SEQUENCE=GGGGATCCTCTTCAAAACTCTAA
PRIMER_RIGHT_3_SEQUENCE=GCCACAACAGAGTGAACGATAG
PRIMER_LEFT_3=24,23
PRIMER_RIGHT_3=244,22
PRIMER_LEFT_3_TM=59.958
PRIMER_RIGHT_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=1.3542
PRIMER_LEFT_4_PENALTY=1.287819
PRIMER_RIGHT_4_PENALTY=0.066394
PRIMER_LEFT_4_SEQUENCE=GGGGATCCTCTTCAAAACTCTA
PRIMER_RIGHT_4_SEQUENCE=ACGTTGGGACACTTTTTAGCAT
PRIMER_LEFT_4=24,22
PRIMER_RIGHT_4=205,22
PRIMER_LEFT_4_TM=58.712
PRIMER_RIGHT_4_TM=59.934
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_03540 Ps_contig_mira-and-tgicl-ass_franssen_30342
SEQUENCE=CGGGGCCTTCTAAATTTTTTTTNTACGACGAATCAATCATTCATTCATTCAGAGATCGCGCTCTCGCTCTTTCTCTCTCTCACTCCTCATTCTCTCTCTTTCTCTCTCTTCTTGCACCTCGAAGCTTCTTCTCTGGTGTGAATCACAGAGGAAGTCAGCTCAGAACCTCCTCACTCCTCAGTGACAGAACTAAACAGAGAGGGTACCCTTGTGCTGCTGATGGCTACTAATGAAAATCTTCCGCCTAATGTAATAAAGCAACTTGCCAAGGAGTTGAAGAATCTTGATGAAACCCCCCCTGAGGGCATTAAAGTTGTGGTGAATGATGATGATTTCTCAACTATATATGCTGACATAGATGGCCCAGCTGGGACTCCTTATGAGAATGGAGTTTTCCGCATGAAGTTGCTACTGTCCCGTGATTTCCCACATTCACCTCCTAAAGGATATTTTTTGACCAAGATTTTTCATCCAAATGTTGCAAACAATGGGGAGATTTGTGTCAACACACTTAAAAAGGATTGGAATCCTAGCCTTGGATTACGGCATGTTCTTATTGTTGTTAGGTGTCTATTGATTGAACCGTTTCCGGAGTCAGCTTTAAATGAACTGGCTGGTAAACTACTGCTTGAAGATTACGAGGAGTATGCCAGACATGCCAGAATTTACACTGGCATTCATGCAAAACCAAAGGCCAAGTTCAAAAGTGGAGCTATAAGCGAATCAACTACTGCTCTGAATGTTGATCAGACGAACACCTCAGTGCTTAATGCTGATATCAAAACCATGCCGTCAAGTGCCGCTTTGCCACCATTGTCGTCAAGTGCCGCTTTGCCACCATTGTCGCTGCCCTCGACCACTGCAGCAAGAGGTGCTAGTCAGGATCAGGCAGTAGGTATTCTGACTGAGTCTTCTGCTAATCGTTCTACTGCTGCTGCCGTAGTTTCTGCTGCTCATGCGCCACAGAAGAAGGAAGTTGGAACTGCCAAAGCTCAGGCAGACAAGAAGAAGCTAGATGCAAGAAAGAAAAGTTTGAAAAGATTATGATGAAGTTGTTTAATAATCAAACTTTTTTTTTCCTTTGTCTTTAGTTTTTTTATTTGATAGAAAGAAAGAAAGAGGAGAGGGAAGAGGAAATGGATTCAAAATTTGGTTTATGCATGTGTACTATTGAAATGGGTGAAATTGTGTACTATCGATAATTTTTAGGAAAACAAGACTCACCCTTATTTCTATTATTTGCCTTTTGTTTTTTCTTTTTCTAAATTG
TARGET=37,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03541 Ps_contig_mira-and-tgicl-ass_franssen_30342
SEQUENCE=CGGGGCCTTCTAAATTTTTTTTNTACGACGAATCAATCATTCATTCATTCAGAGATCGCGCTCTCGCTCTTTCTCTCTCTCACTCCTCATTCTCTCTCTTTCTCTCTCTTCTTGCACCTCGAAGCTTCTTCTCTGGTGTGAATCACAGAGGAAGTCAGCTCAGAACCTCCTCACTCCTCAGTGACAGAACTAAACAGAGAGGGTACCCTTGTGCTGCTGATGGCTACTAATGAAAATCTTCCGCCTAATGTAATAAAGCAACTTGCCAAGGAGTTGAAGAATCTTGATGAAACCCCCCCTGAGGGCATTAAAGTTGTGGTGAATGATGATGATTTCTCAACTATATATGCTGACATAGATGGCCCAGCTGGGACTCCTTATGAGAATGGAGTTTTCCGCATGAAGTTGCTACTGTCCCGTGATTTCCCACATTCACCTCCTAAAGGATATTTTTTGACCAAGATTTTTCATCCAAATGTTGCAAACAATGGGGAGATTTGTGTCAACACACTTAAAAAGGATTGGAATCCTAGCCTTGGATTACGGCATGTTCTTATTGTTGTTAGGTGTCTATTGATTGAACCGTTTCCGGAGTCAGCTTTAAATGAACTGGCTGGTAAACTACTGCTTGAAGATTACGAGGAGTATGCCAGACATGCCAGAATTTACACTGGCATTCATGCAAAACCAAAGGCCAAGTTCAAAAGTGGAGCTATAAGCGAATCAACTACTGCTCTGAATGTTGATCAGACGAACACCTCAGTGCTTAATGCTGATATCAAAACCATGCCGTCAAGTGCCGCTTTGCCACCATTGTCGTCAAGTGCCGCTTTGCCACCATTGTCGCTGCCCTCGACCACTGCAGCAAGAGGTGCTAGTCAGGATCAGGCAGTAGGTATTCTGACTGAGTCTTCTGCTAATCGTTCTACTGCTGCTGCCGTAGTTTCTGCTGCTCATGCGCCACAGAAGAAGGAAGTTGGAACTGCCAAAGCTCAGGCAGACAAGAAGAAGCTAGATGCAAGAAAGAAAAGTTTGAAAAGATTATGATGAAGTTGTTTAATAATCAAACTTTTTTTTTCCTTTGTCTTTAGTTTTTTTATTTGATAGAAAGAAAGAAAGAGGAGAGGGAAGAGGAAATGGATTCAAAATTTGGTTTATGCATGTGTACTATTGAAATGGGTGAAATTGTGTACTATCGATAATTTTTAGGAAAACAAGACTCACCCTTATTTCTATTATTTGCCTTTTGTTTTTTCTTTTTCTAAATTG
TARGET=72,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03542 Ps_contig_mira-and-tgicl-ass_franssen_30342
SEQUENCE=CGGGGCCTTCTAAATTTTTTTTNTACGACGAATCAATCATTCATTCATTCAGAGATCGCGCTCTCGCTCTTTCTCTCTCTCACTCCTCATTCTCTCTCTTTCTCTCTCTTCTTGCACCTCGAAGCTTCTTCTCTGGTGTGAATCACAGAGGAAGTCAGCTCAGAACCTCCTCACTCCTCAGTGACAGAACTAAACAGAGAGGGTACCCTTGTGCTGCTGATGGCTACTAATGAAAATCTTCCGCCTAATGTAATAAAGCAACTTGCCAAGGAGTTGAAGAATCTTGATGAAACCCCCCCTGAGGGCATTAAAGTTGTGGTGAATGATGATGATTTCTCAACTATATATGCTGACATAGATGGCCCAGCTGGGACTCCTTATGAGAATGGAGTTTTCCGCATGAAGTTGCTACTGTCCCGTGATTTCCCACATTCACCTCCTAAAGGATATTTTTTGACCAAGATTTTTCATCCAAATGTTGCAAACAATGGGGAGATTTGTGTCAACACACTTAAAAAGGATTGGAATCCTAGCCTTGGATTACGGCATGTTCTTATTGTTGTTAGGTGTCTATTGATTGAACCGTTTCCGGAGTCAGCTTTAAATGAACTGGCTGGTAAACTACTGCTTGAAGATTACGAGGAGTATGCCAGACATGCCAGAATTTACACTGGCATTCATGCAAAACCAAAGGCCAAGTTCAAAAGTGGAGCTATAAGCGAATCAACTACTGCTCTGAATGTTGATCAGACGAACACCTCAGTGCTTAATGCTGATATCAAAACCATGCCGTCAAGTGCCGCTTTGCCACCATTGTCGTCAAGTGCCGCTTTGCCACCATTGTCGCTGCCCTCGACCACTGCAGCAAGAGGTGCTAGTCAGGATCAGGCAGTAGGTATTCTGACTGAGTCTTCTGCTAATCGTTCTACTGCTGCTGCCGTAGTTTCTGCTGCTCATGCGCCACAGAAGAAGGAAGTTGGAACTGCCAAAGCTCAGGCAGACAAGAAGAAGCTAGATGCAAGAAAGAAAAGTTTGAAAAGATTATGATGAAGTTGTTTAATAATCAAACTTTTTTTTTCCTTTGTCTTTAGTTTTTTTATTTGATAGAAAGAAAGAAAGAGGAGAGGGAAGAGGAAATGGATTCAAAATTTGGTTTATGCATGTGTACTATTGAAATGGGTGAAATTGTGTACTATCGATAATTTTTAGGAAAACAAGACTCACCCTTATTTCTATTATTTGCCTTTTGTTTTTTCTTTTTCTAAATTG
TARGET=1106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1255
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.088198
PRIMER_LEFT_SEQUENCE=TGCTAGTCAGGATCAGGCAGTA
PRIMER_RIGHT_SEQUENCE=AATCCATTTCCTCTTCCCTCTC
PRIMER_LEFT=872,22
PRIMER_RIGHT=1142,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.912
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.1255
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.088198
PRIMER_LEFT_1_SEQUENCE=TGCTAGTCAGGATCAGGCAGTA
PRIMER_RIGHT_1_SEQUENCE=GAATCCATTTCCTCTTCCCTCT
PRIMER_LEFT_1=872,22
PRIMER_RIGHT_1=1143,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.912
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.1876
PRIMER_LEFT_2_PENALTY=0.099438
PRIMER_RIGHT_2_PENALTY=0.088198
PRIMER_LEFT_2_SEQUENCE=AGAGGTGCTAGTCAGGATCAGG
PRIMER_RIGHT_2_SEQUENCE=AATCCATTTCCTCTTCCCTCTC
PRIMER_LEFT_2=867,22
PRIMER_RIGHT_2=1142,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=59.912
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.1876
PRIMER_LEFT_3_PENALTY=0.099438
PRIMER_RIGHT_3_PENALTY=0.088198
PRIMER_LEFT_3_SEQUENCE=AGAGGTGCTAGTCAGGATCAGG
PRIMER_RIGHT_3_SEQUENCE=GAATCCATTTCCTCTTCCCTCT
PRIMER_LEFT_3=867,22
PRIMER_RIGHT_3=1143,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=59.912
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.5362
PRIMER_LEFT_4_PENALTY=0.447971
PRIMER_RIGHT_4_PENALTY=0.088198
PRIMER_LEFT_4_SEQUENCE=AGCAAGAGGTGCTAGTCAGGAT
PRIMER_RIGHT_4_SEQUENCE=AATCCATTTCCTCTTCCCTCTC
PRIMER_LEFT_4=863,22
PRIMER_RIGHT_4=1142,22
PRIMER_LEFT_4_TM=59.552
PRIMER_RIGHT_4_TM=59.912
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_03543 Ps_contig_mira-and-tgicl-ass_franssen_30347
SEQUENCE=CTACTAACAATTTCTAATNCAACACAAGANAAAACCCTGGAGAATGTTATGTGAACATATTGTGATATACTAACTCATTTCAATATAGTAATATTATACACTGAATTTGAGTGCAATGCCAGTTCCTACTGGAGGGAATTGTAAAACAAAATCAAAACTACTGAAACACCTATAATAATAGGGAAAGCTGAAACCAAGTATAGTGCTCCATAGCCCAACACCGAAGGCACTTCTGTTTCATCGTCTGTATCCACATCTTCAATTTCAGCAGCCACATCCCAGTCAATGTTTAGTCTCTTTGCAACCACTTTAGGATCGATTCCAGTGTCAGTGGTTTTTGCATATAGAGATTTTTTTGGCTTTTTCTTTTTTATTTCAACCTCTAAATTAGGCCATGCATTCATTTCCTCAGCAAGCATCTGGAGAACAACTTTATCCTTTGGAATTTTCCCTTCATTCACTTCTTCCAAAGCGGTAACTAGATCATCTCGTGTTGGGCCATCAAGATCTTCAATACCGAGTAATCTTTTTCTCTCAACATCTCGGGGATCCTCAATCATTATGGTTAGTTTGACCTCCCCGAATAGCATGTCCCGGTTTTTTTTAAGCAGCTCCAAAACCCTTTTGATTTGGGCAATATATTCAAGGTCTTGAGGGGTTGGTTCTTCTTCTTCATCATCATCATCATCCTCCTCAACATCATCATCATCATCATTCAGTTGCTGTGTAGAGGAAACACGTAGGTCAGAATCTTCATTCCCAACAGAGGCAACGACGGAAGAAAGTATGGTTGATGTTCGGCGATGAGTGATGCTAAGCTTCGTGAACTGTGCTCCGTTGCTAAGGAAGGAGTGAGAGTTATGATAACAGCGAAAGGTTGGAAGAGAATGTGATTGTGAAGATGAAATTGAAGGAAGTGTTAATGGCAACGGTTGTGATGATGATGATGACAGTGCCATTGTTAACAACCTTCGCTGCTTTTTCTATCCTATCATCTTTCTTACAAACATATCCTAATG
TARGET=663,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2874
PRIMER_LEFT_PENALTY=0.129103
PRIMER_RIGHT_PENALTY=0.158317
PRIMER_LEFT_SEQUENCE=ATTCACTTCTTCCAAAGCGGTA
PRIMER_RIGHT_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT=455,22
PRIMER_RIGHT=739,22
PRIMER_LEFT_TM=60.129
PRIMER_RIGHT_TM=59.842
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.2939
PRIMER_LEFT_1_PENALTY=0.234069
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=CTTTTTCTCTCAACATCTCGGG
PRIMER_RIGHT_1_SEQUENCE=TTGGGAATGAAGATTCTGACCT
PRIMER_LEFT_1=525,22
PRIMER_RIGHT_1=762,22
PRIMER_LEFT_1_TM=60.234
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.3388
PRIMER_LEFT_2_PENALTY=0.180440
PRIMER_RIGHT_2_PENALTY=0.158317
PRIMER_LEFT_2_SEQUENCE=CACTTCTTCCAAAGCGGTAACT
PRIMER_RIGHT_2_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT_2=458,22
PRIMER_RIGHT_2=739,22
PRIMER_LEFT_2_TM=59.820
PRIMER_RIGHT_2_TM=59.842
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.3924
PRIMER_LEFT_3_PENALTY=0.234069
PRIMER_RIGHT_3_PENALTY=0.158317
PRIMER_LEFT_3_SEQUENCE=CTTTTTCTCTCAACATCTCGGG
PRIMER_RIGHT_3_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT_3=525,22
PRIMER_RIGHT_3=739,22
PRIMER_LEFT_3_TM=60.234
PRIMER_RIGHT_3_TM=59.842
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=0.4044
PRIMER_LEFT_4_PENALTY=0.344527
PRIMER_RIGHT_4_PENALTY=0.059875
PRIMER_LEFT_4_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_4_SEQUENCE=TTGGGAATGAAGATTCTGACCT
PRIMER_LEFT_4=639,22
PRIMER_RIGHT_4=762,22
PRIMER_LEFT_4_TM=60.345
PRIMER_RIGHT_4_TM=59.940
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_03544 Ps_contig_mira-and-tgicl-ass_franssen_30347
SEQUENCE=CTACTAACAATTTCTAATNCAACACAAGANAAAACCCTGGAGAATGTTATGTGAACATATTGTGATATACTAACTCATTTCAATATAGTAATATTATACACTGAATTTGAGTGCAATGCCAGTTCCTACTGGAGGGAATTGTAAAACAAAATCAAAACTACTGAAACACCTATAATAATAGGGAAAGCTGAAACCAAGTATAGTGCTCCATAGCCCAACACCGAAGGCACTTCTGTTTCATCGTCTGTATCCACATCTTCAATTTCAGCAGCCACATCCCAGTCAATGTTTAGTCTCTTTGCAACCACTTTAGGATCGATTCCAGTGTCAGTGGTTTTTGCATATAGAGATTTTTTTGGCTTTTTCTTTTTTATTTCAACCTCTAAATTAGGCCATGCATTCATTTCCTCAGCAAGCATCTGGAGAACAACTTTATCCTTTGGAATTTTCCCTTCATTCACTTCTTCCAAAGCGGTAACTAGATCATCTCGTGTTGGGCCATCAAGATCTTCAATACCGAGTAATCTTTTTCTCTCAACATCTCGGGGATCCTCAATCATTATGGTTAGTTTGACCTCCCCGAATAGCATGTCCCGGTTTTTTTTAAGCAGCTCCAAAACCCTTTTGATTTGGGCAATATATTCAAGGTCTTGAGGGGTTGGTTCTTCTTCTTCATCATCATCATCATCCTCCTCAACATCATCATCATCATCATTCAGTTGCTGTGTAGAGGAAACACGTAGGTCAGAATCTTCATTCCCAACAGAGGCAACGACGGAAGAAAGTATGGTTGATGTTCGGCGATGAGTGATGCTAAGCTTCGTGAACTGTGCTCCGTTGCTAAGGAAGGAGTGAGAGTTATGATAACAGCGAAAGGTTGGAAGAGAATGTGATTGTGAAGATGAAATTGAAGGAAGTGTTAATGGCAACGGTTGTGATGATGATGATGACAGTGCCATTGTTAACAACCTTCGCTGCTTTTTCTATCCTATCATCTTTCTTACAAACATATCCTAATG
TARGET=675,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2874
PRIMER_LEFT_PENALTY=0.129103
PRIMER_RIGHT_PENALTY=0.158317
PRIMER_LEFT_SEQUENCE=ATTCACTTCTTCCAAAGCGGTA
PRIMER_RIGHT_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT=455,22
PRIMER_RIGHT=739,22
PRIMER_LEFT_TM=60.129
PRIMER_RIGHT_TM=59.842
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.2939
PRIMER_LEFT_1_PENALTY=0.234069
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=CTTTTTCTCTCAACATCTCGGG
PRIMER_RIGHT_1_SEQUENCE=TTGGGAATGAAGATTCTGACCT
PRIMER_LEFT_1=525,22
PRIMER_RIGHT_1=762,22
PRIMER_LEFT_1_TM=60.234
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.3388
PRIMER_LEFT_2_PENALTY=0.180440
PRIMER_RIGHT_2_PENALTY=0.158317
PRIMER_LEFT_2_SEQUENCE=CACTTCTTCCAAAGCGGTAACT
PRIMER_RIGHT_2_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT_2=458,22
PRIMER_RIGHT_2=739,22
PRIMER_LEFT_2_TM=59.820
PRIMER_RIGHT_2_TM=59.842
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.3924
PRIMER_LEFT_3_PENALTY=0.234069
PRIMER_RIGHT_3_PENALTY=0.158317
PRIMER_LEFT_3_SEQUENCE=CTTTTTCTCTCAACATCTCGGG
PRIMER_RIGHT_3_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT_3=525,22
PRIMER_RIGHT_3=739,22
PRIMER_LEFT_3_TM=60.234
PRIMER_RIGHT_3_TM=59.842
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=0.4044
PRIMER_LEFT_4_PENALTY=0.344527
PRIMER_RIGHT_4_PENALTY=0.059875
PRIMER_LEFT_4_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_4_SEQUENCE=TTGGGAATGAAGATTCTGACCT
PRIMER_LEFT_4=639,22
PRIMER_RIGHT_4=762,22
PRIMER_LEFT_4_TM=60.345
PRIMER_RIGHT_4_TM=59.940
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_03545 Ps_contig_mira-and-tgicl-ass_franssen_30347
SEQUENCE=CTACTAACAATTTCTAATNCAACACAAGANAAAACCCTGGAGAATGTTATGTGAACATATTGTGATATACTAACTCATTTCAATATAGTAATATTATACACTGAATTTGAGTGCAATGCCAGTTCCTACTGGAGGGAATTGTAAAACAAAATCAAAACTACTGAAACACCTATAATAATAGGGAAAGCTGAAACCAAGTATAGTGCTCCATAGCCCAACACCGAAGGCACTTCTGTTTCATCGTCTGTATCCACATCTTCAATTTCAGCAGCCACATCCCAGTCAATGTTTAGTCTCTTTGCAACCACTTTAGGATCGATTCCAGTGTCAGTGGTTTTTGCATATAGAGATTTTTTTGGCTTTTTCTTTTTTATTTCAACCTCTAAATTAGGCCATGCATTCATTTCCTCAGCAAGCATCTGGAGAACAACTTTATCCTTTGGAATTTTCCCTTCATTCACTTCTTCCAAAGCGGTAACTAGATCATCTCGTGTTGGGCCATCAAGATCTTCAATACCGAGTAATCTTTTTCTCTCAACATCTCGGGGATCCTCAATCATTATGGTTAGTTTGACCTCCCCGAATAGCATGTCCCGGTTTTTTTTAAGCAGCTCCAAAACCCTTTTGATTTGGGCAATATATTCAAGGTCTTGAGGGGTTGGTTCTTCTTCTTCATCATCATCATCATCCTCCTCAACATCATCATCATCATCATTCAGTTGCTGTGTAGAGGAAACACGTAGGTCAGAATCTTCATTCCCAACAGAGGCAACGACGGAAGAAAGTATGGTTGATGTTCGGCGATGAGTGATGCTAAGCTTCGTGAACTGTGCTCCGTTGCTAAGGAAGGAGTGAGAGTTATGATAACAGCGAAAGGTTGGAAGAGAATGTGATTGTGAAGATGAAATTGAAGGAAGTGTTAATGGCAACGGTTGTGATGATGATGATGACAGTGCCATTGTTAACAACCTTCGCTGCTTTTTCTATCCTATCATCTTTCTTACAAACATATCCTAATG
TARGET=698,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2874
PRIMER_LEFT_PENALTY=0.129103
PRIMER_RIGHT_PENALTY=0.158317
PRIMER_LEFT_SEQUENCE=ATTCACTTCTTCCAAAGCGGTA
PRIMER_RIGHT_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT=455,22
PRIMER_RIGHT=739,22
PRIMER_LEFT_TM=60.129
PRIMER_RIGHT_TM=59.842
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.2939
PRIMER_LEFT_1_PENALTY=0.234069
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=CTTTTTCTCTCAACATCTCGGG
PRIMER_RIGHT_1_SEQUENCE=TTGGGAATGAAGATTCTGACCT
PRIMER_LEFT_1=525,22
PRIMER_RIGHT_1=762,22
PRIMER_LEFT_1_TM=60.234
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.3388
PRIMER_LEFT_2_PENALTY=0.180440
PRIMER_RIGHT_2_PENALTY=0.158317
PRIMER_LEFT_2_SEQUENCE=CACTTCTTCCAAAGCGGTAACT
PRIMER_RIGHT_2_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT_2=458,22
PRIMER_RIGHT_2=739,22
PRIMER_LEFT_2_TM=59.820
PRIMER_RIGHT_2_TM=59.842
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.3924
PRIMER_LEFT_3_PENALTY=0.234069
PRIMER_RIGHT_3_PENALTY=0.158317
PRIMER_LEFT_3_SEQUENCE=CTTTTTCTCTCAACATCTCGGG
PRIMER_RIGHT_3_SEQUENCE=CGTGTTTCCTCTACACAGCAAC
PRIMER_LEFT_3=525,22
PRIMER_RIGHT_3=739,22
PRIMER_LEFT_3_TM=60.234
PRIMER_RIGHT_3_TM=59.842
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=0.4044
PRIMER_LEFT_4_PENALTY=0.344527
PRIMER_RIGHT_4_PENALTY=0.059875
PRIMER_LEFT_4_SEQUENCE=TATTCAAGGTCTTGAGGGGTTG
PRIMER_RIGHT_4_SEQUENCE=TTGGGAATGAAGATTCTGACCT
PRIMER_LEFT_4=639,22
PRIMER_RIGHT_4=762,22
PRIMER_LEFT_4_TM=60.345
PRIMER_RIGHT_4_TM=59.940
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_03546 Ps_contig_mira-and-tgicl-ass_franssen_30347
SEQUENCE=CTACTAACAATTTCTAATNCAACACAAGANAAAACCCTGGAGAATGTTATGTGAACATATTGTGATATACTAACTCATTTCAATATAGTAATATTATACACTGAATTTGAGTGCAATGCCAGTTCCTACTGGAGGGAATTGTAAAACAAAATCAAAACTACTGAAACACCTATAATAATAGGGAAAGCTGAAACCAAGTATAGTGCTCCATAGCCCAACACCGAAGGCACTTCTGTTTCATCGTCTGTATCCACATCTTCAATTTCAGCAGCCACATCCCAGTCAATGTTTAGTCTCTTTGCAACCACTTTAGGATCGATTCCAGTGTCAGTGGTTTTTGCATATAGAGATTTTTTTGGCTTTTTCTTTTTTATTTCAACCTCTAAATTAGGCCATGCATTCATTTCCTCAGCAAGCATCTGGAGAACAACTTTATCCTTTGGAATTTTCCCTTCATTCACTTCTTCCAAAGCGGTAACTAGATCATCTCGTGTTGGGCCATCAAGATCTTCAATACCGAGTAATCTTTTTCTCTCAACATCTCGGGGATCCTCAATCATTATGGTTAGTTTGACCTCCCCGAATAGCATGTCCCGGTTTTTTTTAAGCAGCTCCAAAACCCTTTTGATTTGGGCAATATATTCAAGGTCTTGAGGGGTTGGTTCTTCTTCTTCATCATCATCATCATCCTCCTCAACATCATCATCATCATCATTCAGTTGCTGTGTAGAGGAAACACGTAGGTCAGAATCTTCATTCCCAACAGAGGCAACGACGGAAGAAAGTATGGTTGATGTTCGGCGATGAGTGATGCTAAGCTTCGTGAACTGTGCTCCGTTGCTAAGGAAGGAGTGAGAGTTATGATAACAGCGAAAGGTTGGAAGAGAATGTGATTGTGAAGATGAAATTGAAGGAAGTGTTAATGGCAACGGTTGTGATGATGATGATGACAGTGCCATTGTTAACAACCTTCGCTGCTTTTTCTATCCTATCATCTTTCTTACAAACATATCCTAATG
TARGET=936,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1413
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.081412
PRIMER_LEFT_SEQUENCE=GAATCTTCATTCCCAACAGAGG
PRIMER_RIGHT_SEQUENCE=ATAGAAAAAGCAGCGAAGGTTG
PRIMER_LEFT=747,22
PRIMER_RIGHT=986,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=59.919
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.1413
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=0.081412
PRIMER_LEFT_1_SEQUENCE=AGGTCAGAATCTTCATTCCCAA
PRIMER_RIGHT_1_SEQUENCE=ATAGAAAAAGCAGCGAAGGTTG
PRIMER_LEFT_1=741,22
PRIMER_RIGHT_1=986,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=59.919
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=0.2105
PRIMER_LEFT_2_PENALTY=0.129103
PRIMER_RIGHT_2_PENALTY=0.081412
PRIMER_LEFT_2_SEQUENCE=ATAACAGCGAAAGGTTGGAAGA
PRIMER_RIGHT_2_SEQUENCE=ATAGAAAAAGCAGCGAAGGTTG
PRIMER_LEFT_2=863,22
PRIMER_RIGHT_2=986,22
PRIMER_LEFT_2_TM=60.129
PRIMER_RIGHT_2_TM=59.919
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=0.2105
PRIMER_LEFT_3_PENALTY=0.129103
PRIMER_RIGHT_3_PENALTY=0.081412
PRIMER_LEFT_3_SEQUENCE=GATAACAGCGAAAGGTTGGAAG
PRIMER_RIGHT_3_SEQUENCE=ATAGAAAAAGCAGCGAAGGTTG
PRIMER_LEFT_3=862,22
PRIMER_RIGHT_3=986,22
PRIMER_LEFT_3_TM=60.129
PRIMER_RIGHT_3_TM=59.919
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=0.2397
PRIMER_LEFT_4_PENALTY=0.158317
PRIMER_RIGHT_4_PENALTY=0.081412
PRIMER_LEFT_4_SEQUENCE=GTTGCTGTGTAGAGGAAACACG
PRIMER_RIGHT_4_SEQUENCE=ATAGAAAAAGCAGCGAAGGTTG
PRIMER_LEFT_4=718,22
PRIMER_RIGHT_4=986,22
PRIMER_LEFT_4_TM=59.842
PRIMER_RIGHT_4_TM=59.919
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_03547 Ps_contig_mira-and-tgicl-ass_franssen_30348
SEQUENCE=TTCAATGCAAGTTACATGCTGATTNNNRNNAAAGTGAATGTAACATGTATTGTTTGGATTGTATGAATGGTCCTATATGTTCTCTCTGTCTTTCTTACCATAAAGAACATCGTTTTATTCAGATCCGAAGATCTTCATACCATGATGTGATTAGGGTGAATGAGATTCAGAAGGTATTGGACATCACTGGTGTTCAAACGTATGTTATCAACAGTGCAAGAGTTGTGTTTTTGAATGAAAGGCCTCAGCCAAGGCCGGGAAAAGGTGTCACAAACACTTGTGAAGTTTGCGAGCGTAGTCTTCTCGATTCCTTCCGGTTCTGCTCTCTCGGTTGCAAGATTGTAGGAACATCTAAGAATTTCCAGAAGAAAAAGAAACAAATAACAGCAATGGCATCAGATTCAGAAGATTCATACAGTAGCAACAACAACATTCACAGCCGTCACAAGAAAATCAAAGTTCAAAGCTTCACACCATCAACACCTCCTCCAACTTCTGTTAATTACAGAACAGCCAAGAGAAGAAAGGGAATTCCACATAGAGCCCCATTGGGGATAGATAGTTGGTACTATTAATAGTTGGTAATTTTATTTTATTTGCTATAATGACCCTATGTCAAGTACTATTTTTTTAAAAATGTTTTTAGTGGGTGTATATAGCAAAAATCCAATAGAAGTGGAAAAAGTTGGGTAGTAGAAAATAAAAGACACCTGTGGAAGAATAGTTGGTGGTGCATTTAGCAAAAAAAGAAGAAGAAGGGGGTAAGTAACCCGTGCGTGTCTAGCAAAAGAAGTAAGTTTACTAGGTTTGTTTTACTAAAGTAATTATACCTAAGTTTTGTGGTTGAGGATGTAAATGTGGGTTAGTAAGATGTGGGAGAAAAGGAAATGAAAATGTACTAGTTGAAAAAGGTGGAATGTCAATATATGGAAATACAATAATAAAATGGATCTTATCTTATGTTATTTTATTTGTATAATATTTGTATAATAATCAATCTCTTCATAAGAATTTTATTAGAATTAAATTTTTATTTTCCATTATTCCTTCATATCCTTTTCTATTTATTTTTCTAATTTTTTTTCTTAATGTGTTAACTTATTGTGAGTGGTTGTGAATGCTGGTGGTGGTGGCGATGT
TARGET=747,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03548 Ps_contig_mira-and-tgicl-ass_franssen_30348
SEQUENCE=TTCAATGCAAGTTACATGCTGATTNNNRNNAAAGTGAATGTAACATGTATTGTTTGGATTGTATGAATGGTCCTATATGTTCTCTCTGTCTTTCTTACCATAAAGAACATCGTTTTATTCAGATCCGAAGATCTTCATACCATGATGTGATTAGGGTGAATGAGATTCAGAAGGTATTGGACATCACTGGTGTTCAAACGTATGTTATCAACAGTGCAAGAGTTGTGTTTTTGAATGAAAGGCCTCAGCCAAGGCCGGGAAAAGGTGTCACAAACACTTGTGAAGTTTGCGAGCGTAGTCTTCTCGATTCCTTCCGGTTCTGCTCTCTCGGTTGCAAGATTGTAGGAACATCTAAGAATTTCCAGAAGAAAAAGAAACAAATAACAGCAATGGCATCAGATTCAGAAGATTCATACAGTAGCAACAACAACATTCACAGCCGTCACAAGAAAATCAAAGTTCAAAGCTTCACACCATCAACACCTCCTCCAACTTCTGTTAATTACAGAACAGCCAAGAGAAGAAAGGGAATTCCACATAGAGCCCCATTGGGGATAGATAGTTGGTACTATTAATAGTTGGTAATTTTATTTTATTTGCTATAATGACCCTATGTCAAGTACTATTTTTTTAAAAATGTTTTTAGTGGGTGTATATAGCAAAAATCCAATAGAAGTGGAAAAAGTTGGGTAGTAGAAAATAAAAGACACCTGTGGAAGAATAGTTGGTGGTGCATTTAGCAAAAAAAGAAGAAGAAGGGGGTAAGTAACCCGTGCGTGTCTAGCAAAAGAAGTAAGTTTACTAGGTTTGTTTTACTAAAGTAATTATACCTAAGTTTTGTGGTTGAGGATGTAAATGTGGGTTAGTAAGATGTGGGAGAAAAGGAAATGAAAATGTACTAGTTGAAAAAGGTGGAATGTCAATATATGGAAATACAATAATAAAATGGATCTTATCTTATGTTATTTTATTTGTATAATATTTGTATAATAATCAATCTCTTCATAAGAATTTTATTAGAATTAAATTTTTATTTTCCATTATTCCTTCATATCCTTTTCTATTTATTTTTCTAATTTTTTTTCTTAATGTGTTAACTTATTGTGAGTGGTTGTGAATGCTGGTGGTGGTGGCGATGT
TARGET=1122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03549 Ps_contig_mira-and-tgicl-ass_franssen_30354
SEQUENCE=AGTTTTTTAGTACATTTCTTTTTTCTTGCTATTTAATTACAAGAGGTTGGAAAAAAATTATGGGATCTTCTTCTTATTCTACATAGTCAAATTTGAGGACTAGTATGTTTTCGTCTTTTTAACATTACTGAAAAGAATAAAAGAACAATGATATTTTCCCCTTGATACATTACATTACATTACTCCCATGGACTCAATGAACACTCTTCAGGCAAAGTCGGAAAACGTTCCGAATCGTTGCAATAATCATAAATCACAAGGTTTCTTTGAACCCAAGCATAGTCCATTTTCTGATCATCTGATAGATGCCATGAATCATATTGATCCCACCAATTTTTTGTTGTGGTTGCAACACATTTAGGATATGGATCTTCCCATTGACAAGCATCAACACTGAAGTCTTTGTAGGATGACACAAATGGTGCTAATTTCCAGTTTGTTTTTTCAAGTCCACCTCTCGTCGCCCATTCGTCCGCGTTCCATACGCTTGAAAACAAGTACATTGGTTTCTCATTCGGGAAAAAATTGTTTGGTTTTTCGTTGTTCTTGAATACTCTTATCGGAACTCTATCCACAAAAAACAACTAGCTGGTGATTATTCCACAGGATTGAATATGTGTGATAATCCTCAGTTGGATCAAACCATAACATATGTCTCATCTCGCGATTACCGGTTCCGTTCTTGTACACATTTGTCTGAATCAAATATGGTTGTCCTGTTCTGTTTCCCAAAAACTCAAAATCAATTTCATCTCTTGTTGGCCCTGCACCATTTTCTGAACACATATAATAAGCTGTGACAACACCAGCAGAATCACCTCCTACCAATTTCAGCTTCATACTAAACCACCCAAACCTATATCTTTGTTTGGTTTGAAATCCACAACCTGTGTTATTGTCTAAGGAGAGATTCCAGATCTGTCCATCATCAGAGGTAGTGAAATGATCTTCAGACCACATAATGCTGAAATTATCTTCAAAAGATCCCTTAGTCGTAGCTGCTTCAGCTGAATCATAATAAGCAGAAGCTATGATAGCTGCAATGAAAAGAACAAGGGAAAATGAAGAACATGTTCTTGCATCCATTTTGTGTTTCTCAGCTTTGGATCCCCCGAGCTCGAAGCTTGGAAGTTTATTAAATAATAGTAGCTTTTGAAAATGGTTTCTGAATATACAGTTGT
TARGET=166,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03550 Ps_contig_mira-and-tgicl-ass_franssen_30366
SEQUENCE=AGTTGTGAATTCTTTGAATTTCTTTCTGTTACTATAAGTTAAATTTCAGAAAAAGAAAAACACTTTTTATAGACACATTAACCTGAGTTTTTCATATAGAAAATAAAGTAAGGGCCACAGTAGGAGCAACAACAATCCAACAGTTACATACTTTCTATCACAAACTACAAAGCCAATATGCCAATAGGACAAACTAAGTGACAAAGTTTATGAGAATATTGCTATTTTACTTTTGCAGAAAGGATTACATATTTATTATTGAAGGATATGGCAGTATAGATCAGTGCTTGGTTTTTGGCTTCTTTACAATCATCACGGTGCAATGAGCATGATGAGCACAATAGTCACTCACACTACCTAAAACCGCCCTTTTTATAGCCCCATAACCGTGACTACCCACAACCAATATTGAAGCATGGTGTTTCTCCACTGTATCACAGAGAACATTTCTAGCATCACCTTCCACCACCTCCACTATTACATCATTTACCGATTTCTTAATGCAAAGCTGTTTAGCACTATCAGCAACTCTAGCAGCAACCCTTTTCAAATCAGAATCGACCATCGGCAAAACCTCAGCAGCTCCTGCATACACAGGGCCAGCGAGACCAACAGAAGAAGTAGCGGAAGGTCTAGCGTGAAGAAGAACAAGCTTGAAAAGGGAATTGTTGTCGTTGAAGAAATGATCGAGAGTCCATTTGAGAGCGTAAGCACTGTGTTCACTCTCGTCGATTGCAACTACCATCACTGGTTGTTGTGGTTTCTCTGATTGATCTGAACTCGCCATTCTGAGAAGACAAACCGAATTTTGATACCGAATTAGTTACCCTTTCTCTCTTCTATATATCTCTTTTTCTTCACTTGCTCCAGACGCCNCRAAACGTTGCCTTATATATATATCCCGCGTACTCTGCGTTTGTATTACCTACTTGTCTTT
TARGET=890,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03551 Ps_contig_mira-and-tgicl-ass_franssen_30367
SEQUENCE=AACGCAGAGTACGTCGGGGATGTCACTCTTCTATTTCTCTTTGCTAGCTATATAGCTTTTTAGAAAGTTTGTATAGAAGAACAATATGGGTGCACTTTATTTCTTGATCTCAAACTTTTGCACTTCACCAAACACAAAAACCAAACGCAAACCAATGCAGACAGTTGAGATCAAAGTGAAAATGGATTGTGATGGATGTGAAAGAAGAGTCAGAAATGCAGTGGCTACAATGAAAGGTGTGAAATCAGTGGAGATAAACAGAAAGCAAAGCAAAGTGACAGTGAATGGTTTTGTTGATCCAAACAAAGTGTTGAAGAGGATAAAAAGAACTGGAAAAAAGAGAGCTGAATTTTGGCCTTATGTTCCACAACATGTGGTAACCTTTCCTCATCTCTCAGGAATTTATGATAAAAGAGCACCAGCAGGATATGTAAAAAATGCAAGAAATACATTCCATCGGCGCTGGCGGTACCAGGTTCTTCGGCGGCGGAGGCGGCGACCGGAGACTCAGGCCACATCTCAACAACAACAACAACCAGAACAACAACCAAGCCTTGAACTGTCCTCGCTGCGACTCCATCAATACGAAATTCTGTTACTACAATAACTACAACCTCTCCCAGCCTCGTCACTTCTGCAAAAACTGCCGTCGGTACTGGACCAAAGGCGGCGTCCTCCGTAACGTCCCTGTCGGTGGCGGTTGCCGTAAATCCAAACGCTCAAACAAGCCGAAAAACTCTTCCTCATCGGAAATCGCTGATTTGCCGACTACTCCACCGGAGCCGGAGAACAAATCGAACTCTCACTCGAGCAGCGAGAGTTCCAGCCTCACCGCGGCAGCGGTAACGGAAGCAGTCTCAGAACCAACGCCANCAAACTCCAACTCAAAAAATCAACACCGGTTTGGAAAACGA
TARGET=521,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0987
PRIMER_LEFT_PENALTY=0.023913
PRIMER_RIGHT_PENALTY=0.074807
PRIMER_LEFT_SEQUENCE=GCTGAATTTTGGCCTTATGTTC
PRIMER_RIGHT_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_LEFT=343,22
PRIMER_RIGHT=560,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=0.1705
PRIMER_LEFT_1_PENALTY=0.095684
PRIMER_RIGHT_1_PENALTY=0.074807
PRIMER_LEFT_1_SEQUENCE=AGCAAAGCAAAGTGACAGTGAA
PRIMER_RIGHT_1_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_LEFT_1=263,22
PRIMER_RIGHT_1=560,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.075
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.1705
PRIMER_LEFT_2_PENALTY=0.095684
PRIMER_RIGHT_2_PENALTY=0.074807
PRIMER_LEFT_2_SEQUENCE=AAGCAAAGCAAAGTGACAGTGA
PRIMER_RIGHT_2_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_LEFT_2=262,22
PRIMER_RIGHT_2=560,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=0.2313
PRIMER_LEFT_3_PENALTY=0.156479
PRIMER_RIGHT_3_PENALTY=0.074807
PRIMER_LEFT_3_SEQUENCE=CTGAATTTTGGCCTTATGTTCC
PRIMER_RIGHT_3_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_LEFT_3=344,22
PRIMER_RIGHT_3=560,22
PRIMER_LEFT_3_TM=59.844
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=0.2331
PRIMER_LEFT_4_PENALTY=0.158297
PRIMER_RIGHT_4_PENALTY=0.074807
PRIMER_LEFT_4_SEQUENCE=AAAGAGCACCAGCAGGATATGT
PRIMER_RIGHT_4_SEQUENCE=AGTTCAAGGCTTGGTTGTTGTT
PRIMER_LEFT_4=410,22
PRIMER_RIGHT_4=560,22
PRIMER_LEFT_4_TM=60.158
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_03552 Ps_contig_mira-and-tgicl-ass_franssen_30377
SEQUENCE=ACCGATCAAATAANNYATCAGATAGTCAACATTTCATGAAAAACTAATGTGAAAATCGATTTTATTATACATCATCTGTGCAAAGAAAGTATCTACTTATCATTGCAACCTCACCAGAGAATAAAGGGAACAGATTGATACTCAAATAGTTCAAAGTTCAAACAAACTAGAGTTGTAATCAGTAATTAGATGACCAATTAGCAGCACTTCCAACTCTTGGCATGCCAGTCGCTTCCTTCTTAGAATCAGCATCCACCTTGAAAGTCGAACCACACACCTGACCAGAACTATCAATCCTTGGTATCGGCTTCAACTCGTTGAGTGGATGCTCGAAACTCCTCAAACCTCCCGATGTTGTAAAGAAGCATCCTTTAGGGAATGGAGCATAAGATTTGCCACAGCTCTTGCGAACAACATCAATCTCGTCTGAAAGAACTAGACTTTCATCAGCATCAATTCCCCAGAAGAAAGGAACACTCCCGTCAGCATCAGCAGCAACAAACGCAGTTTGCGAACTGCTATCAAACAGGATGAACGCAAATTTGCCTTGGAAATCTCTCACAACTTGATCAGCGGGATAGGGACCCCGATCCCTTAAGGTCCTGTAAGCCTCTATGACAATGGTCACCTCATTTGCTGATTTGTTCAATCCATATTGTTGTTTAAGATTAGCAACATTATCGATGTGGCCTTGAAACAAGCCGAAGATGTCATCCACAACTGCAAACAGCCTTGGGAGTAATGGGTTGTTTTTGTGATGAGAATAGGCTAAGAAAGCGGAAGAAGCAAGGTTAAGGTTAACAGAAGAAGGGTGGAGAGATGCGAAATGTTGACCCAAAAAACCATCTTTGAGTGATGAAACTGGGTTTGATTCAGGACTCTGTAAACCCTCGGGACTTTTGGCCACAGATTTGTTGAAAACCGCTAACATTTTTAATTTTTCTTTTTCTCTGGATCAAAAATGGATTCTGAAAGAACAGAAGAAGTCACNNAGTTGCTGAAGTTGAAGATGAAGAGAAAGTGAAGACATAAAAGTAGATAGAAGCTACTGGCGGATTGTTAGAAGACGAGAAGAAAGAAAGAAGAAAAAGAAAAGAAGAACTCTAGGCGTTAGAATAACCTACGTGTCTT
TARGET=1072,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03553 Ps_contig_mira-and-tgicl-ass_franssen_30378
SEQUENCE=TTTTACTTTTTTTACTTTCCAAAAAAAAGGGGTGGGGTGTGCAAGTGTGCAAAACGCGCGAGTTGACTCAGTGTGAAACCACCATTGAGTTGGCGGCGAGTTTCCAAGCAGAGGTTGCAATCAATGGCCTGGTGTGCCATCCTAACATGAGACAACCATCATTCTCTTCCACCTTGTAACCATCCCCACCTGCGAAAAGTGCCAACAACATACTCGCTTGCTTGAACGCGTTGGATCCCAAATGAACCGGAGAAAACCCAGCTGAACCGAACCGGTTCCTCCACTGGTTCAGTGTTTCGTGACGCTCGACTCGGTCCGTTCCTTCACACGCCACCACGTTGCAGATTTGCTTCCCGAGGTAAACCTCTGACATAGCCTTATCCTGCGGTTCAACCAATGAACTCTCCAGCGAGTCAAACAGCGTCGAGTAATAGTGAAGCGACTCAGTAAACCGGTCGAGAAAAGCCGGTCCGTTGTGATTTGCTTCTTGCTCCACGACGGTGACAATCTCCGGCCGAATCTGACGGATAACAGAAAACACCTTCTCAAGCGCACCAGGGCGCGCGTTGAGTTTATGAAGCTCGAAAACAGAGTTAACTGCGACGGACTCAGTCTCCGGTGACCGGAGTTCAAGCATGGAAGCATCAAGATCAGCAAGACTGTTAGCAACAAAACCACGGTACTCAAACTGAACATGAATCGTCTGCGCAAACTGAGCAAGCCTCCAACCAACCTGTTGGAGATGGTCGGAGTTATCCGACGCCGGAGGACCGATTCCGGTGAGACGAAAAGCAGGAGGACCGCCAGGACGCAAGGCAAGTGCCTGCATAAGCGCCGGCCACTGCATCCCTTGATTGATAGAAAAATCAATAACATGAACACGCGATTTTCCTTGAAAAGCTTCAAGAATAGCTTGATTCGCAGTGAAGTGAGCGAACTTCAGGTAAGGACAAGTTTCATAGAAATGGATCTGAAGCGAATCGGAGACAGAATGCTGTGGAAACACATCGTAGATTCTTCGTGCCAAACCTATTGCGAAATAGGTAGCCACTTTTCTCATAGCACCTTCTTGCGACACCGCTAGGTTTCCGATCTGTTTCACAAGAGCTTCCGCCACAGGTCGATTATTCTGTTCAACAGCTTCAGCGCAAGCCATTAAAGTATGAACAAGAATAATCTCTTTCTCCTGCGTTTCAACAACCATAACAACGGGACGAGTTGACTCGGTGGAAGAAGAAGAACCTACTCGTTTGACTCGTTTCCGAGAGGGAAGAGACTCCTCTGTCTGTGAATCAGTGTTGGCGTAGATAGCTTTACCGGGAATGGCGTTAAGGTCGTTATCGGAGGAAGAGGAAACAGAGGGATTAGGTTGAGAGTCAAAATTGGAAAGCATAGTTTGAAGCCAGTTAGAAATATCAGCAGGATTGTAATGAACGGTGTCGTTTGAGAGGTGTTGAGCGATGGTGGCTTCATCTTGATCTTGAAAATTACCCATAGCTTGTTCAAGTTGTTCAAGCTTTTGAGCAACTTCAGCCATGTCTGATGATTTCACCTTGTAACCAACCACCGCTAAAAGCTCATCCATACCACCGTCGTCTTCCCAACAAATCTCCGATTTACCAGTCATACTCGCATCTTCGTGTTCTTGGTTATGCTCTCTCTTCATCTTCTTACACTTCTCTCTCTCNCTCACNCACAAGTTCTATATTCTTGTTTTCTGATTGTAATTTGTGACTTTCTCTTTTCTTTCTCTGTCTTTTTTTTTCTTCTCACTACTGCTTCTAAACTTTCTTCATC
TARGET=1230,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3421
PRIMER_LEFT_PENALTY=0.310317
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=CGTTTCAACAACCATAACAACG
PRIMER_RIGHT_SEQUENCE=AAGATCAAGATGAAGCCACCAT
PRIMER_LEFT=1189,22
PRIMER_RIGHT=1481,22
PRIMER_LEFT_TM=60.310
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.3776
PRIMER_LEFT_1_PENALTY=0.210886
PRIMER_RIGHT_1_PENALTY=0.166752
PRIMER_LEFT_1_SEQUENCE=GCCACTTTTCTCATAGCACCTT
PRIMER_RIGHT_1_SEQUENCE=CGGTAAAGCTATCTACGCCAAC
PRIMER_LEFT_1=1046,22
PRIMER_RIGHT_1=1318,22
PRIMER_LEFT_1_TM=59.789
PRIMER_RIGHT_1_TM=60.167
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.3850
PRIMER_LEFT_2_PENALTY=0.310317
PRIMER_RIGHT_2_PENALTY=0.074695
PRIMER_LEFT_2_SEQUENCE=CGTTTCAACAACCATAACAACG
PRIMER_RIGHT_2_SEQUENCE=ATCCCTCTGTTTCCTCTTCCTC
PRIMER_LEFT_2=1189,22
PRIMER_RIGHT_2=1364,22
PRIMER_LEFT_2_TM=60.310
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=176
PRIMER_PAIR_PENALTY_3=0.4473
PRIMER_LEFT_3_PENALTY=0.372557
PRIMER_RIGHT_3_PENALTY=0.074695
PRIMER_LEFT_3_SEQUENCE=AATCTCTTTCTCCTGCGTTTCA
PRIMER_RIGHT_3_SEQUENCE=ATCCCTCTGTTTCCTCTTCCTC
PRIMER_LEFT_3=1174,22
PRIMER_RIGHT_3=1364,22
PRIMER_LEFT_3_TM=60.373
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=0.4473
PRIMER_LEFT_4_PENALTY=0.372557
PRIMER_RIGHT_4_PENALTY=0.074695
PRIMER_LEFT_4_SEQUENCE=ATCTCTTTCTCCTGCGTTTCAA
PRIMER_RIGHT_4_SEQUENCE=ATCCCTCTGTTTCCTCTTCCTC
PRIMER_LEFT_4=1175,22
PRIMER_RIGHT_4=1364,22
PRIMER_LEFT_4_TM=60.373
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_03554 Ps_contig_mira-and-tgicl-ass_franssen_30378
SEQUENCE=TTTTACTTTTTTTACTTTCCAAAAAAAAGGGGTGGGGTGTGCAAGTGTGCAAAACGCGCGAGTTGACTCAGTGTGAAACCACCATTGAGTTGGCGGCGAGTTTCCAAGCAGAGGTTGCAATCAATGGCCTGGTGTGCCATCCTAACATGAGACAACCATCATTCTCTTCCACCTTGTAACCATCCCCACCTGCGAAAAGTGCCAACAACATACTCGCTTGCTTGAACGCGTTGGATCCCAAATGAACCGGAGAAAACCCAGCTGAACCGAACCGGTTCCTCCACTGGTTCAGTGTTTCGTGACGCTCGACTCGGTCCGTTCCTTCACACGCCACCACGTTGCAGATTTGCTTCCCGAGGTAAACCTCTGACATAGCCTTATCCTGCGGTTCAACCAATGAACTCTCCAGCGAGTCAAACAGCGTCGAGTAATAGTGAAGCGACTCAGTAAACCGGTCGAGAAAAGCCGGTCCGTTGTGATTTGCTTCTTGCTCCACGACGGTGACAATCTCCGGCCGAATCTGACGGATAACAGAAAACACCTTCTCAAGCGCACCAGGGCGCGCGTTGAGTTTATGAAGCTCGAAAACAGAGTTAACTGCGACGGACTCAGTCTCCGGTGACCGGAGTTCAAGCATGGAAGCATCAAGATCAGCAAGACTGTTAGCAACAAAACCACGGTACTCAAACTGAACATGAATCGTCTGCGCAAACTGAGCAAGCCTCCAACCAACCTGTTGGAGATGGTCGGAGTTATCCGACGCCGGAGGACCGATTCCGGTGAGACGAAAAGCAGGAGGACCGCCAGGACGCAAGGCAAGTGCCTGCATAAGCGCCGGCCACTGCATCCCTTGATTGATAGAAAAATCAATAACATGAACACGCGATTTTCCTTGAAAAGCTTCAAGAATAGCTTGATTCGCAGTGAAGTGAGCGAACTTCAGGTAAGGACAAGTTTCATAGAAATGGATCTGAAGCGAATCGGAGACAGAATGCTGTGGAAACACATCGTAGATTCTTCGTGCCAAACCTATTGCGAAATAGGTAGCCACTTTTCTCATAGCACCTTCTTGCGACACCGCTAGGTTTCCGATCTGTTTCACAAGAGCTTCCGCCACAGGTCGATTATTCTGTTCAACAGCTTCAGCGCAAGCCATTAAAGTATGAACAAGAATAATCTCTTTCTCCTGCGTTTCAACAACCATAACAACGGGACGAGTTGACTCGGTGGAAGAAGAAGAACCTACTCGTTTGACTCGTTTCCGAGAGGGAAGAGACTCCTCTGTCTGTGAATCAGTGTTGGCGTAGATAGCTTTACCGGGAATGGCGTTAAGGTCGTTATCGGAGGAAGAGGAAACAGAGGGATTAGGTTGAGAGTCAAAATTGGAAAGCATAGTTTGAAGCCAGTTAGAAATATCAGCAGGATTGTAATGAACGGTGTCGTTTGAGAGGTGTTGAGCGATGGTGGCTTCATCTTGATCTTGAAAATTACCCATAGCTTGTTCAAGTTGTTCAAGCTTTTGAGCAACTTCAGCCATGTCTGATGATTTCACCTTGTAACCAACCACCGCTAAAAGCTCATCCATACCACCGTCGTCTTCCCAACAAATCTCCGATTTACCAGTCATACTCGCATCTTCGTGTTCTTGGTTATGCTCTCTCTTCATCTTCTTACACTTCTCTCTCTCNCTCACNCACAAGTTCTATATTCTTGTTTTCTGATTGTAATTTGTGACTTTCTCTTTTCTTTCTCTGTCTTTTTTTTTCTTCTCACTACTGCTTCTAAACTTTCTTCATC
TARGET=1678,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03555 Ps_contig_mira-and-tgicl-ass_franssen_30379
SEQUENCE=TTACCTATCCAAAACCTTTTTCTTCTTGAATTATGCAAATAGAAAAAGCTTTAGCACACTCAAACTTATCATTAAAATTCTCACAACAACTCACAAATCAGAAAGAAAAAGAAAAACTTTTAAACCTATCTCTAATACTATACACAACAATTAACATGAAGTAAACTATAGTTAGCACATTGAATTACATTACTTTCAATCCATCTCTTGAGCTCTTGTAGGGTCAATTAACAATCTCTTACATAGCTTCCAAGAAAGTGCATTGCAAGTGCCTATGGCAAGTGATTAAGTGATCATTTGTTAGGCCAGCTGCCTGCATAAATGAATGAACCACTGTTGGACCAACAAACCTAAAACCTCTCCTAATCATGTCTTTGCTTATGCTTTCTGATTTTGATGTCTTCACTGGAATCTTGTGGCCAAATTTGTATTGAGTTGAGATTGGTTTTTGGTTCACAAATCCCCATATGTATTTGTCAAATGAACCAAACACTTTTTTAACTTGTAAAATTTGGTTAGCATTGTCAACAACTCCTCTAACTTTGCTTATATCAATGCCATATTCTGAACTAATAGATATCATTTGTTTATCAGTGAAGTTGGCCACAATTTCTGCATCAAATTCTGAAAATGCAACCCTGAAATCTAGGCGTTTTTTTAAGGTTGAGGTCCAATCAGATCCAACTTGAGCACCACTTAGAATTAGAAGTTCAAACAACATCTTGTCATCATGAACAGGAACTCCCCATTCTTCATCATGATAAGCAATATAGATAGGATCTGAATTGGCTGTGATAAAGCTGCACCTCTTCTCCTCTTCATTACTAGTCTTTAAATCAAGAGCACTTGAAGGATCAATAGGAAAAACTCTTTCAAATTTCGCAGACTTCGATCTTCCATAATGAGCAATCTTCATTTTCCTTTGTGCCTGTTGAAGTGCCATCTGCTCCCTTCTCCCTGCAGCTATACTTCCTGGAGAATCAGTGATCGAAGAAGAAGAATAACTCAAAGAAGCTTCAACACCATAAGACCCTTCTTTGAAACTCTTTGATTTCTTTCTCTCTAAACATGGAGTTTTCATTGTGTTTTTTGGTATAACAATCTTTTCACAACTTGAGTTCATTCCATTGTTGTCATTTCCTCTCTTTATAGCTAAGAATGGTCTAGTAGGAGATTTTGGCTTTGGAGAAATTGGTGGTGACAATGAAGTAGTAGTAGTAGTAGTAGAAGTCTTGTTTGGAAGTGGTGGTGAAAAAAGTGATTTTGATGTTAAGTTTTTTCATTGAGTTTCTTCTTTACAAGTATTTGGAAACAAGAACATTGGTTGCATGGTAGGTTGAAGTTACTTGTCTTCATTAGAAATTAACTAAGAAAC
TARGET=990,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1845
PRIMER_LEFT_PENALTY=0.122569
PRIMER_RIGHT_PENALTY=0.061961
PRIMER_LEFT_SEQUENCE=TTCTCCCTGCAGCTATACTTCC
PRIMER_RIGHT_SEQUENCE=ACCACCACTTCCAAACAAGACT
PRIMER_LEFT=951,22
PRIMER_RIGHT=1249,22
PRIMER_LEFT_TM=59.877
PRIMER_RIGHT_TM=59.938
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=0.2862
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.163658
PRIMER_LEFT_1_SEQUENCE=TTCTCCCTGCAGCTATACTTCC
PRIMER_RIGHT_1_SEQUENCE=TCATTGTCACCACCAATTTCTC
PRIMER_LEFT_1=951,22
PRIMER_RIGHT_1=1206,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=59.836
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.3194
PRIMER_LEFT_2_PENALTY=0.122569
PRIMER_RIGHT_2_PENALTY=0.196811
PRIMER_LEFT_2_SEQUENCE=TTCTCCCTGCAGCTATACTTCC
PRIMER_RIGHT_2_SEQUENCE=TCTCCAAAGCCAAAATCTCCTA
PRIMER_LEFT_2=951,22
PRIMER_RIGHT_2=1188,22
PRIMER_LEFT_2_TM=59.877
PRIMER_RIGHT_2_TM=60.197
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.4665
PRIMER_LEFT_3_PENALTY=0.404566
PRIMER_RIGHT_3_PENALTY=0.061961
PRIMER_LEFT_3_SEQUENCE=CTCCCTGCAGCTATACTTCCTG
PRIMER_RIGHT_3_SEQUENCE=ACCACCACTTCCAAACAAGACT
PRIMER_LEFT_3=953,22
PRIMER_RIGHT_3=1249,22
PRIMER_LEFT_3_TM=60.405
PRIMER_RIGHT_3_TM=59.938
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.4902
PRIMER_LEFT_4_PENALTY=0.016312
PRIMER_RIGHT_4_PENALTY=0.473868
PRIMER_LEFT_4_SEQUENCE=CTGCACCTCTTCTCCTCTTCAT
PRIMER_RIGHT_4_SEQUENCE=AAGGGTCTTATGGTGTTGAAGC
PRIMER_LEFT_4=800,22
PRIMER_RIGHT_4=1034,22
PRIMER_LEFT_4_TM=60.016
PRIMER_RIGHT_4_TM=59.526
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_03556 Ps_contig_mira-and-tgicl-ass_franssen_30379
SEQUENCE=TTACCTATCCAAAACCTTTTTCTTCTTGAATTATGCAAATAGAAAAAGCTTTAGCACACTCAAACTTATCATTAAAATTCTCACAACAACTCACAAATCAGAAAGAAAAAGAAAAACTTTTAAACCTATCTCTAATACTATACACAACAATTAACATGAAGTAAACTATAGTTAGCACATTGAATTACATTACTTTCAATCCATCTCTTGAGCTCTTGTAGGGTCAATTAACAATCTCTTACATAGCTTCCAAGAAAGTGCATTGCAAGTGCCTATGGCAAGTGATTAAGTGATCATTTGTTAGGCCAGCTGCCTGCATAAATGAATGAACCACTGTTGGACCAACAAACCTAAAACCTCTCCTAATCATGTCTTTGCTTATGCTTTCTGATTTTGATGTCTTCACTGGAATCTTGTGGCCAAATTTGTATTGAGTTGAGATTGGTTTTTGGTTCACAAATCCCCATATGTATTTGTCAAATGAACCAAACACTTTTTTAACTTGTAAAATTTGGTTAGCATTGTCAACAACTCCTCTAACTTTGCTTATATCAATGCCATATTCTGAACTAATAGATATCATTTGTTTATCAGTGAAGTTGGCCACAATTTCTGCATCAAATTCTGAAAATGCAACCCTGAAATCTAGGCGTTTTTTTAAGGTTGAGGTCCAATCAGATCCAACTTGAGCACCACTTAGAATTAGAAGTTCAAACAACATCTTGTCATCATGAACAGGAACTCCCCATTCTTCATCATGATAAGCAATATAGATAGGATCTGAATTGGCTGTGATAAAGCTGCACCTCTTCTCCTCTTCATTACTAGTCTTTAAATCAAGAGCACTTGAAGGATCAATAGGAAAAACTCTTTCAAATTTCGCAGACTTCGATCTTCCATAATGAGCAATCTTCATTTTCCTTTGTGCCTGTTGAAGTGCCATCTGCTCCCTTCTCCCTGCAGCTATACTTCCTGGAGAATCAGTGATCGAAGAAGAAGAATAACTCAAAGAAGCTTCAACACCATAAGACCCTTCTTTGAAACTCTTTGATTTCTTTCTCTCTAAACATGGAGTTTTCATTGTGTTTTTTGGTATAACAATCTTTTCACAACTTGAGTTCATTCCATTGTTGTCATTTCCTCTCTTTATAGCTAAGAATGGTCTAGTAGGAGATTTTGGCTTTGGAGAAATTGGTGGTGACAATGAAGTAGTAGTAGTAGTAGTAGAAGTCTTGTTTGGAAGTGGTGGTGAAAAAAGTGATTTTGATGTTAAGTTTTTTCATTGAGTTTCTTCTTTACAAGTATTTGGAAACAAGAACATTGGTTGCATGGTAGGTTGAAGTTACTTGTCTTCATTAGAAATTAACTAAGAAAC
TARGET=1208,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1845
PRIMER_LEFT_PENALTY=0.122569
PRIMER_RIGHT_PENALTY=0.061961
PRIMER_LEFT_SEQUENCE=TTCTCCCTGCAGCTATACTTCC
PRIMER_RIGHT_SEQUENCE=ACCACCACTTCCAAACAAGACT
PRIMER_LEFT=951,22
PRIMER_RIGHT=1249,22
PRIMER_LEFT_TM=59.877
PRIMER_RIGHT_TM=59.938
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=0.2486
PRIMER_LEFT_1_PENALTY=0.186600
PRIMER_RIGHT_1_PENALTY=0.061961
PRIMER_LEFT_1_SEQUENCE=TGGAGAATCAGTGATCGAAGAA
PRIMER_RIGHT_1_SEQUENCE=ACCACCACTTCCAAACAAGACT
PRIMER_LEFT_1=973,22
PRIMER_RIGHT_1=1249,22
PRIMER_LEFT_1_TM=59.813
PRIMER_RIGHT_1_TM=59.938
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=277
PRIMER_PAIR_PENALTY_2=0.4460
PRIMER_LEFT_2_PENALTY=0.163658
PRIMER_RIGHT_2_PENALTY=0.282356
PRIMER_LEFT_2_SEQUENCE=GAGAAATTGGTGGTGACAATGA
PRIMER_RIGHT_2_SEQUENCE=AACTTCAACCTACCATGCAACC
PRIMER_LEFT_2=1185,22
PRIMER_RIGHT_2=1343,22
PRIMER_LEFT_2_TM=59.836
PRIMER_RIGHT_2_TM=60.282
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.4665
PRIMER_LEFT_3_PENALTY=0.404566
PRIMER_RIGHT_3_PENALTY=0.061961
PRIMER_LEFT_3_SEQUENCE=CTCCCTGCAGCTATACTTCCTG
PRIMER_RIGHT_3_SEQUENCE=ACCACCACTTCCAAACAAGACT
PRIMER_LEFT_3=953,22
PRIMER_RIGHT_3=1249,22
PRIMER_LEFT_3_TM=60.405
PRIMER_RIGHT_3_TM=59.938
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.4792
PRIMER_LEFT_4_PENALTY=0.196811
PRIMER_RIGHT_4_PENALTY=0.282356
PRIMER_LEFT_4_SEQUENCE=TAGGAGATTTTGGCTTTGGAGA
PRIMER_RIGHT_4_SEQUENCE=AACTTCAACCTACCATGCAACC
PRIMER_LEFT_4=1167,22
PRIMER_RIGHT_4=1343,22
PRIMER_LEFT_4_TM=60.197
PRIMER_RIGHT_4_TM=60.282
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_03557 Ps_contig_mira-and-tgicl-ass_franssen_30382
SEQUENCE=TTCAACGCAGAGTACTCGGGGAGTTTGTTGCCTGTGTCTGTGTTGGATCTACTACCGCCCAATATCAACTTGTCCCAAAGCCACCATGACTGCTCAAACTCAAGAAGAGCTTCTTGCTGCGCATCTCGAACAGCAAAAGATCCATCATGATGAGCCTGTAGTCGAGGATGATGATGATGAGGAAGACGAGGATGATGATGATGATGAGGAAGATGATGATGTAGAAGGACTAGAAGGTGATGCTTCTGGTAGGTCCAAACAGACCAGAAGTGAAAAGAAGAGCCGCAAGGCAATGCTTAAACTTGGGATGAAACCTGTTACCGGTGTCAGCCGTGTGACAGTCAAGAAGAGCAAGAATATCTTGTTCGTCATTTCAAAGCCAGATGTTTTCAAGAGCCCAACTTCCGACACCTACATTATTTTTGGTGAAGCTAAGATTGAGGACTTGAGTTCACAGCTACAAACTCAGGCAGCAGAGCAGTTCAAGGCTCCTAATTTGAGCAATGTTGGAGCGAAGCCAGAATCCTCTGGTATTGCTCCGGAAGAGGAAGATGTAGACGAGACTGGTGTTGATCCCAAGGATATCGAGTTGGTGGTGACTCAAGCTGGTGTGCCAAGATCAAGAGCTGTTAAAGCTCTCAAAGCTGCCAATGGAGACATTGTTGCTGCCATTATGGAGCTTACTACTTAATGCTCAGAATTGGCAGTGTTTTAGATTTTGGAATTAGGTGGAGCTTATTTTTTCTTTTGTTGGTACTAAATTTTTTGCGCATGTTCAGTTTGAAGTAGTGTTAATCGTTATTATTTGTAACTTTAA
TARGET=167,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3670
PRIMER_LEFT_PENALTY=0.118192
PRIMER_RIGHT_PENALTY=0.248781
PRIMER_LEFT_SEQUENCE=GTTTGTTGCCTGTGTCTGTGTT
PRIMER_RIGHT_SEQUENCE=TTTCACTTCTGGTCTGTTTGGA
PRIMER_LEFT=22,22
PRIMER_RIGHT=274,22
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.751
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.4032
PRIMER_LEFT_1_PENALTY=0.118192
PRIMER_RIGHT_1_PENALTY=0.284978
PRIMER_LEFT_1_SEQUENCE=GTTTGTTGCCTGTGTCTGTGTT
PRIMER_RIGHT_1_SEQUENCE=TTCCTCATCATCATCATCATCC
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=210,22
PRIMER_LEFT_1_TM=60.118
PRIMER_RIGHT_1_TM=59.715
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=0.4194
PRIMER_LEFT_2_PENALTY=0.118192
PRIMER_RIGHT_2_PENALTY=0.301190
PRIMER_LEFT_2_SEQUENCE=GTTTGTTGCCTGTGTCTGTGTT
PRIMER_RIGHT_2_SEQUENCE=ATCATCATCATCCTCGTCTTCC
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=201,22
PRIMER_LEFT_2_TM=60.118
PRIMER_RIGHT_2_TM=60.301
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.4303
PRIMER_LEFT_3_PENALTY=0.118192
PRIMER_RIGHT_3_PENALTY=0.312071
PRIMER_LEFT_3_SEQUENCE=GTTTGTTGCCTGTGTCTGTGTT
PRIMER_RIGHT_3_SEQUENCE=TCATCATCATCATCATCCTCGT
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=206,22
PRIMER_LEFT_3_TM=60.118
PRIMER_RIGHT_3_TM=60.312
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=0.4303
PRIMER_LEFT_4_PENALTY=0.118192
PRIMER_RIGHT_4_PENALTY=0.312071
PRIMER_LEFT_4_SEQUENCE=GTTTGTTGCCTGTGTCTGTGTT
PRIMER_RIGHT_4_SEQUENCE=CATCATCATCATCATCCTCGTC
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=205,22
PRIMER_LEFT_4_TM=60.118
PRIMER_RIGHT_4_TM=60.312
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_03558 Ps_contig_mira-and-tgicl-ass_franssen_30382
SEQUENCE=TTCAACGCAGAGTACTCGGGGAGTTTGTTGCCTGTGTCTGTGTTGGATCTACTACCGCCCAATATCAACTTGTCCCAAAGCCACCATGACTGCTCAAACTCAAGAAGAGCTTCTTGCTGCGCATCTCGAACAGCAAAAGATCCATCATGATGAGCCTGTAGTCGAGGATGATGATGATGAGGAAGACGAGGATGATGATGATGATGAGGAAGATGATGATGTAGAAGGACTAGAAGGTGATGCTTCTGGTAGGTCCAAACAGACCAGAAGTGAAAAGAAGAGCCGCAAGGCAATGCTTAAACTTGGGATGAAACCTGTTACCGGTGTCAGCCGTGTGACAGTCAAGAAGAGCAAGAATATCTTGTTCGTCATTTCAAAGCCAGATGTTTTCAAGAGCCCAACTTCCGACACCTACATTATTTTTGGTGAAGCTAAGATTGAGGACTTGAGTTCACAGCTACAAACTCAGGCAGCAGAGCAGTTCAAGGCTCCTAATTTGAGCAATGTTGGAGCGAAGCCAGAATCCTCTGGTATTGCTCCGGAAGAGGAAGATGTAGACGAGACTGGTGTTGATCCCAAGGATATCGAGTTGGTGGTGACTCAAGCTGGTGTGCCAAGATCAAGAGCTGTTAAAGCTCTCAAAGCTGCCAATGGAGACATTGTTGCTGCCATTATGGAGCTTACTACTTAATGCTCAGAATTGGCAGTGTTTTAGATTTTGGAATTAGGTGGAGCTTATTTTTTCTTTTGTTGGTACTAAATTTTTTGCGCATGTTCAGTTTGAAGTAGTGTTAATCGTTATTATTTGTAACTTTAA
TARGET=191,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3197
PRIMER_LEFT_PENALTY=0.217974
PRIMER_RIGHT_PENALTY=0.101680
PRIMER_LEFT_SEQUENCE=TGATGATGATGAGGAAGACGAG
PRIMER_RIGHT_SEQUENCE=GCTCTTGAAAACATCTGGCTTT
PRIMER_LEFT=168,22
PRIMER_RIGHT=396,22
PRIMER_LEFT_TM=60.218
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.3670
PRIMER_LEFT_1_PENALTY=0.118192
PRIMER_RIGHT_1_PENALTY=0.248781
PRIMER_LEFT_1_SEQUENCE=GTTTGTTGCCTGTGTCTGTGTT
PRIMER_RIGHT_1_SEQUENCE=TTTCACTTCTGGTCTGTTTGGA
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=274,22
PRIMER_LEFT_1_TM=60.118
PRIMER_RIGHT_1_TM=59.751
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.4622
PRIMER_LEFT_2_PENALTY=0.118192
PRIMER_RIGHT_2_PENALTY=0.343977
PRIMER_LEFT_2_SEQUENCE=GTTTGTTGCCTGTGTCTGTGTT
PRIMER_RIGHT_2_SEQUENCE=GGTTTCATCCCAAGTTTAAGCA
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=314,22
PRIMER_LEFT_2_TM=60.118
PRIMER_RIGHT_2_TM=60.344
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.4668
PRIMER_LEFT_3_PENALTY=0.217974
PRIMER_RIGHT_3_PENALTY=0.248781
PRIMER_LEFT_3_SEQUENCE=TGATGATGATGAGGAAGACGAG
PRIMER_RIGHT_3_SEQUENCE=TTTCACTTCTGGTCTGTTTGGA
PRIMER_LEFT_3=168,22
PRIMER_RIGHT_3=274,22
PRIMER_LEFT_3_TM=60.218
PRIMER_RIGHT_3_TM=59.751
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=0.4748
PRIMER_LEFT_4_PENALTY=0.118192
PRIMER_RIGHT_4_PENALTY=0.356592
PRIMER_LEFT_4_SEQUENCE=GTTTGTTGCCTGTGTCTGTGTT
PRIMER_RIGHT_4_SEQUENCE=GACCTACCAGAAGCATCACCTT
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=254,22
PRIMER_LEFT_4_TM=60.118
PRIMER_RIGHT_4_TM=59.643
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_03559 Ps_contig_mira-and-tgicl-ass_franssen_30383
SEQUENCE=ACCTCAATGCTCCTCCAACAAAAGAACATAACCTTATCAAAATCAGCATATAAACTCCTGTACTTCCCATCTTTTCTCCACCTTCAATAACATAACATCTTCATCAGCAACAAACAAACCATGGGAAACAAGATAGGAAAGGAACCTGAGGTTGTCAAAGGTGTAGATTTGGAGAGATACACGGGTCGTTGGTACGAGATTGCTTCTTTTCCTTCGTTTTTCCAACCCAAAAACAGTGAGAACACTAGAGCCACCTATACTCTAAACAGTGATGGAACTGTTCATGTCCTCAACGAGACATGGAATAATGGAAAAAGAAACTCCATAGAGGGAAGTGCTTATAAAGCTAACCCTAATAGCGATGAAGCCAAACTCAAGGTCAAGTTCTTTGTTCCTCCATTCTTGCCAATCATTCCTGTTCTTGGAAACTACTGGATTTTGTACCTTGATGAAGATTATCAATATGCTCTTATTGGAGAGCCTACAAGAAAATATCTTTGGATATTATGCAGACAAACGCATTTGGATGATGAAATTTACAACCAACTTGTTGAGAAAGCTAAGGAAGAAGGGTATGATGATGTGACAACCAAATTGCGCAAGACTCCACAAAGTGATCCTCCACCAGAGTGAGAAAATTAAGCCCCTTTTTGGGAAATAATTAAATAAATAAATTTGTATGGAGATTTCAACAAGTGTTAATTTTATCATGTACTATTAAATAAAGAGTTCAGGAATTTAAATATTGAGTAATTTAAATTTTAATGCTTGTATTGGTTTGTTTTGTGAATAGACTAAGATAAGTTGGCGTTAATTTGATGCTTGAATTGTGTTATTGGTTGTTAAGTTTATAGGATGATTATTAGGTGTTTACTTTTCCTTTTTATTTGTTGACTTTTTTTTGTAAATGGAGATTCCATAATTTATCCTTCATCTAGATCACTCTAGACATGTGGATACGGTTAACTGATGCGAAAAAGATA
TARGET=663,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7985
PRIMER_LEFT_PENALTY=0.110131
PRIMER_RIGHT_PENALTY=1.688418
PRIMER_LEFT_SEQUENCE=ATCCTCCACCAGAGTGAGAAAA
PRIMER_RIGHT_SEQUENCE=CAAGCATCAAATTAACGCCAAC
PRIMER_LEFT=616,22
PRIMER_RIGHT=824,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=61.688
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=1.7985
PRIMER_LEFT_1_PENALTY=0.110131
PRIMER_RIGHT_1_PENALTY=1.688418
PRIMER_LEFT_1_SEQUENCE=TCCTCCACCAGAGTGAGAAAAT
PRIMER_RIGHT_1_SEQUENCE=CAAGCATCAAATTAACGCCAAC
PRIMER_LEFT_1=617,22
PRIMER_RIGHT_1=824,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=61.688
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=1.9316
PRIMER_LEFT_2_PENALTY=0.243148
PRIMER_RIGHT_2_PENALTY=1.688418
PRIMER_LEFT_2_SEQUENCE=GATGATGTGACAACCAAATTGC
PRIMER_RIGHT_2_SEQUENCE=CAAGCATCAAATTAACGCCAAC
PRIMER_LEFT_2=576,22
PRIMER_RIGHT_2=824,22
PRIMER_LEFT_2_TM=60.243
PRIMER_RIGHT_2_TM=61.688
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=2.0839
PRIMER_LEFT_3_PENALTY=0.395499
PRIMER_RIGHT_3_PENALTY=1.688418
PRIMER_LEFT_3_SEQUENCE=AGACTCCACAAAGTGATCCTCC
PRIMER_RIGHT_3_SEQUENCE=CAAGCATCAAATTAACGCCAAC
PRIMER_LEFT_3=601,22
PRIMER_RIGHT_3=824,22
PRIMER_LEFT_3_TM=59.605
PRIMER_RIGHT_3_TM=61.688
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=2.1025
PRIMER_LEFT_4_PENALTY=0.414033
PRIMER_RIGHT_4_PENALTY=1.688418
PRIMER_LEFT_4_SEQUENCE=ACTCCACAAAGTGATCCTCCAC
PRIMER_RIGHT_4_SEQUENCE=CAAGCATCAAATTAACGCCAAC
PRIMER_LEFT_4=603,22
PRIMER_RIGHT_4=824,22
PRIMER_LEFT_4_TM=60.414
PRIMER_RIGHT_4_TM=61.688
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_03560 Ps_contig_mira-and-tgicl-ass_franssen_30389
SEQUENCE=GTAAACTTAACTCATTTATATACTAGAATRAAAAAGTTAAAAAGTTTTTTCGAACATTAGATTCGTAGTTACTATCATCAGTATAATTGACAAAACTTAATTCACTTGAAAAATAAAGTTCCACTACTAAATCCATCATCCTTATTAGTCCTATTTGAAAAATTACCCATTAGCACATTGCACAACAAAAATAACAGATGCAGCAAGTATTTCTGCAAGGAGAAGGACCTGCAGTACTTCATAACAAAGTGAACCTATGACAATATTTTCTATCATTATATCATCCTAAGAGTGCTCGCGTATATTTTTTCCCTTCAACAATCTGCCTCTATTATCAGTACCCATGCTAACCTCCCAATCACATAATCCACGTTAATTGGACAAAATATTGATACTACTCAACTTGTTTGCTTTCAGTAACAGGAAGGTAGACACCTTCAGCTGCCAACCAGATATTATCAGCGCGCTTTTCACGAATACCGCAAACCTCCTGCTGTCCCCCAGCAATCCGACTGTATTTCTTATCATGACTATGTAGATAGCCTGAAGTGTCAACATGCTGAAGCCGAATCTTTTGATCCTGCTTCCAAGTCTTTCCACTTCCTTCTATAAAAAGCCTCCAATAGTCCCCGGTGTCAGAATCATTTTCTCCACCAAAGCAGCTGACCTCAAGATTGCCTGATATTGGGGACGCATGCAAATGGCTGTGCAACCACTTCCTTGTCCTCATATGCTGAAATCTAACTATTGTGCCACTTTTAATGGTGTCGCCTTGTTTAGCTGAAGTTCCAGGCTCAGGCCTAACAATCCAGTAACTGTTGGAATCATCAACCGCAGGAAAACCAGTAACCGATTGCTGTCCACTGCCAGAACCATACGGCACATCATGAGAATGCAACCTAAATTTCGTCTTCTCATGCATAAGCTTTATAACCGAACCATAAGTAATCTGAACCTCGACGCCTTCGGAGGAAGCTGCGGAAGCAGAAGCGGTGGAAGACGGAGAAACGTCAGGATCGAGAGTGAGAAAGAGAAAAATAGCGAGAGCGAAGAATCCAAGAGCCATTACGAAGAGAAACGCGATGAACCAGTTACGTTGCCAGATCGGAAGATCCGATTCGGAATGGACGGTTGATTCTCTGTATATGATTCGGGTCGACCCGGTTTGTTGCGAAGATGAAGAAGAAGAAGAAGAAGGTTTTCTTCGTGATAGTCCTCCCTGGTGTTGTGACCCTGAGGAAGCCACTGTGATAGAAATAATTTAGTGCATTCCACACTTCACCGTGAAAGAAATTTCGTTTGCGATTTGGTTATGTTATGGTGGTGATTGTGATCGCCTAATTCAACCCCTTCTTGCACGGTAACGTAACNAGTCCCCCGA
TARGET=1179,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03561 Ps_contig_mira-and-tgicl-ass_franssen_30394
SEQUENCE=CGGGGTTACTCGCGGATGCTACGCACGTGTATCTTTCTTCCCAAAGCGCTCTGTTCTGAACTTGAGCAACATCATTTTCACATCAACAAACCATGGCATTCTTGTTGAACAAATCAACCCTAGCTTCTCATTTTCGATCCACATCTCAGAAAATCGAAGATCCCGTTTCCCTCTCGCGCCGTCAGTTCCATGTTGAACCCGGGACTCGTGAAAAAGCTCTCTTGGCAGAAGATTCAGCTCTCAAGCCATTCAAATCATATAAGCAGAGTGTAAAAAAGCTCCGGAAAATTGGGGATGTTTTAACTATTGTTGTTGTTGCAGGATGCTGTTATGAAATATATGTCAAGGCTTCTACGAGAGAAGAGGCTCGCAAACAGTAGTGTTCTGAAAGTGCAGAATGAGATGAACATTTGGATGTCAGTTTTTTTTACATTGTTTTTTTATGGCAACAATTTGGATGTTATGATAGCCATGTTAAAATAAACTGAAAGCACTAGAACCCTAATATTGCTTGTGCATGAGTAGTTTTTTCACCACTCAAGATGTGCGCGTTCTGATAATATTGATTGCCGGATCATAGTCGAGAGAGTAGTTGTGGAGTGTGTTATTTATTGTGATTCTGTAGTCAAGCTTTCACTTAAATGTTCTATGAATCTGGTGATCATATAGTAACTTACTGCTGAGCAAAGATCTTTTGCCAGTTTCTTTTNAAAAAAACTTT
TARGET=307,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0251
PRIMER_LEFT_PENALTY=0.010294
PRIMER_RIGHT_PENALTY=0.014792
PRIMER_LEFT_SEQUENCE=AGATTCAGCTCTCAAGCCATTC
PRIMER_RIGHT_SEQUENCE=TCATGCACAAGCAATATTAGGG
PRIMER_LEFT=229,22
PRIMER_RIGHT=520,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=59.985
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=0.0251
PRIMER_LEFT_1_PENALTY=0.010294
PRIMER_RIGHT_1_PENALTY=0.014792
PRIMER_LEFT_1_SEQUENCE=GAAGATTCAGCTCTCAAGCCAT
PRIMER_RIGHT_1_SEQUENCE=TCATGCACAAGCAATATTAGGG
PRIMER_LEFT_1=227,22
PRIMER_RIGHT_1=520,22
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=59.985
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=0.0920
PRIMER_LEFT_2_PENALTY=0.010294
PRIMER_RIGHT_2_PENALTY=0.081670
PRIMER_LEFT_2_SEQUENCE=AGATTCAGCTCTCAAGCCATTC
PRIMER_RIGHT_2_SEQUENCE=ACTACTGTTTGCGAGCCTCTTC
PRIMER_LEFT_2=229,22
PRIMER_RIGHT_2=380,22
PRIMER_LEFT_2_TM=59.990
PRIMER_RIGHT_2_TM=60.082
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.0920
PRIMER_LEFT_3_PENALTY=0.010294
PRIMER_RIGHT_3_PENALTY=0.081670
PRIMER_LEFT_3_SEQUENCE=GAAGATTCAGCTCTCAAGCCAT
PRIMER_RIGHT_3_SEQUENCE=ACTACTGTTTGCGAGCCTCTTC
PRIMER_LEFT_3=227,22
PRIMER_RIGHT_3=380,22
PRIMER_LEFT_3_TM=59.990
PRIMER_RIGHT_3_TM=60.082
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.1357
PRIMER_LEFT_4_PENALTY=0.054021
PRIMER_RIGHT_4_PENALTY=0.081670
PRIMER_LEFT_4_SEQUENCE=ACTCGTGAAAAAGCTCTCTTGG
PRIMER_RIGHT_4_SEQUENCE=ACTACTGTTTGCGAGCCTCTTC
PRIMER_LEFT_4=203,22
PRIMER_RIGHT_4=380,22
PRIMER_LEFT_4_TM=60.054
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_03562 Ps_contig_mira-and-tgicl-ass_franssen_30395
SEQUENCE=TTCCATGTTGAACCCGGGACTCGTGAAAAAGCTCTCTTGGCAGAAGATTCAGCTCTCAAGCCATTCAAATCATATAAGCAGAGTGTAAAAAAGCTCCGGAAAATTGGGGATGTTTTAACTATTGTTGTTGTTGCAGGATGCTGTTATGAAATATATGTCAAGGCTTCTACGAGAGAAGAGGCTCGCAAACAGTAGTGTTCTGAAAGTGCAGAATGAGATGAACATTTGGATGTCTAGTTTTTTTTACATTTTGTTTTTTTATGGCAAACTAATTTGGATTGTTATGATAGCCATAGTTAAAAATAACTGAAAGCAACTAACGAACCCTAATTATTGTCTTGTGCATGGTAGTTTAGTTTTTTCCACCTACTACAAGNTGTGCGTCGTTAAC
TARGET=122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0920
PRIMER_LEFT_PENALTY=0.010294
PRIMER_RIGHT_PENALTY=0.081670
PRIMER_LEFT_SEQUENCE=AGATTCAGCTCTCAAGCCATTC
PRIMER_RIGHT_SEQUENCE=ACTACTGTTTGCGAGCCTCTTC
PRIMER_LEFT=44,22
PRIMER_RIGHT=195,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=0.0920
PRIMER_LEFT_1_PENALTY=0.010294
PRIMER_RIGHT_1_PENALTY=0.081670
PRIMER_LEFT_1_SEQUENCE=GAAGATTCAGCTCTCAAGCCAT
PRIMER_RIGHT_1_SEQUENCE=ACTACTGTTTGCGAGCCTCTTC
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=195,22
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=60.082
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=154
PRIMER_PAIR_PENALTY_2=0.1357
PRIMER_LEFT_2_PENALTY=0.054021
PRIMER_RIGHT_2_PENALTY=0.081670
PRIMER_LEFT_2_SEQUENCE=ACTCGTGAAAAAGCTCTCTTGG
PRIMER_RIGHT_2_SEQUENCE=ACTACTGTTTGCGAGCCTCTTC
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=195,22
PRIMER_LEFT_2_TM=60.054
PRIMER_RIGHT_2_TM=60.082
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=0.1713
PRIMER_LEFT_3_PENALTY=0.054021
PRIMER_RIGHT_3_PENALTY=0.117273
PRIMER_LEFT_3_SEQUENCE=ACTCGTGAAAAAGCTCTCTTGG
PRIMER_RIGHT_3_SEQUENCE=CAGAACACTACTGTTTGCGAGC
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=201,22
PRIMER_LEFT_3_TM=60.054
PRIMER_RIGHT_3_TM=60.117
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.1907
PRIMER_LEFT_4_PENALTY=0.010294
PRIMER_RIGHT_4_PENALTY=0.180440
PRIMER_LEFT_4_SEQUENCE=AGATTCAGCTCTCAAGCCATTC
PRIMER_RIGHT_4_SEQUENCE=AGGGTTCGTTAGTTGCTTTCAG
PRIMER_LEFT_4=44,22
PRIMER_RIGHT_4=327,22
PRIMER_LEFT_4_TM=59.990
PRIMER_RIGHT_4_TM=59.820
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_03563 Ps_contig_mira-and-tgicl-ass_franssen_30405
SEQUENCE=AAGACAGTGGGTATCAACGCAGAGTACGTCGGGGGACTCCTTCCTAGAGCGTCGTTACTCTGCGGAAAAAAAATCCAAAACACTTATTAGAGCTCTAAAATTTCTAAGCCTGTTGAGATTTCACACTGAATCTCAACAAGTTTGAGCTAAAAACCACTACCTGGATAGCAACCCCGACAAAACGAAAGAAAATCGCACAAGAAGAAGAAGCAGAGATTAGGACAAGAAGAAAGCAGAGCAAGGGAAAACGATAAGGCATACCTTATACACTGCAGATCCGGTTGGTTTATGCGCTTTGCCTTTGTATAGATCCATGACTCTTAGGGCATTTTGATTCTGTTTTGATGTATGGCGATAACTGTTGATTTATTTTTTTCATTTGTTGGATGAATTGTGTTTGTTGCAAACCATGTGAGGTTTGATTCCGACTTTGAAGGTGCTGAGAACGAAAATCTGGAGTTCGGTTTTAAAAGGCGGAATTTGTTTCGCCTCTAAGGACTCCGATCCTTCTAATTGTTGTTCATTTAAAGTTAGAATCACGTCTGGAATTCGATTTGGGGTAGGATTGGTGTTTGAGATCTTAGAAGTCATGTTGGTTTGTTAAATTTCTGGAAAATCGCTCAAGAACTTTCAAGTGTTCTTGAGAAGTGATTATGGTATTATGCAGGCTTTTATGGNTGTTTTTACTTTTTRGTTTTTAGGGNAAGGGAAGGAGTTCGGT
TARGET=199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03564 Ps_contig_mira-and-tgicl-ass_franssen_30406
SEQUENCE=ACAACGRGAAGGAGCCAAAGGCTCCAATCTCTTCTTCTTCCTGTACACTCGGTGACGCCAAAGGGGAGAGACGTACAGTGAGAATCGAACCTGAATGGAGATCCAGGCCGCGTCGGTTTCGTCACCGACGGCGGTGATCCGAAGTGGTGGAATTAACCTCGTTCTAAACCTCACAACAAATCAATCGGAACGGAAACGACAGAGATTTACCGGCGACGGCGAAGACAAATCGGAAGCAAACGTTGATTTCGTGTTTAGTTGTCCTATCCTTCACCTTCTTGCTTTTCCTTCGATCGTAAAATCTCTCCTGCTACATTTTGTTTTTAGATCGATGATCCTTCGGTACCTTCACGGCGGAGCCTTGGCGAGCAGGTGTAGCGGTCCTCCGATCGAAAACAGGTACCGTCCGTGTTTCTTCTTCTTATCGTCGCTCTCGTATGTGGTTGACTTCGCAGAGTTTGGATTCGAATTTACTGTTGCTGTACATCGATCTCCGTTTGCTGTTTGGATTTGCAGTGGTTGATGTGGAGAGGAGGTGGACGGTGGTGCGATTGGTAGAGTTGTTGTAGACTGGAAATGGACGTTTGATTGAAGGTTTTGTTTAAAGGTTGAAAGTTTGTTACGGTGATGACTGATTGGAAGAAGAAGAAGAAGAATCTGGAATCGTTGCAGAGATTTGGAGAGTGAAGAATGAAGAAGAGGAAAAAATGAAGATGTGGAAGAGGATTGAAGATGGAGATTGCAATGGATTAAAAAAAAACCCTCCTTTCTGTTAGATACTATTAGATTTATATGGATGAGGATCTTGATTAGAAGAAGTTAGGAAGTTTGTTAGGTTTTTCGGTTATTGGATTTGTTAGTTGCAGAATTTTTGGTTTTAATTATGCAGAATTCTGTTTTGGAATGGCTTACGTCGACGGTGTCGTTGTTGTTAGGCTTGGCTAGTGTTGGTTTGCACTGTTGTAAGTAGTTTGTATTTCTATTTTTTCATGGCTATTGGTGCGTAGCCTGGTGCAATTTTTCTGTTGTTTTTATCTTTGGCATTCACTCTTGGTGGCATGTACTTTTGGCTGGCTTGCACGTCATTTTGGTGTGACCATGCTGCAACATTCATTTAACAATTGGAACATGAATTATAATAGATATGAATGACTTACTAGRAAACC
TARGET=639,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03565 Ps_contig_mira-and-tgicl-ass_franssen_30407
SEQUENCE=TTAACTTGTCCATAATTTAAAAACCTAAAAAAACTTTAAATTAGCAACTAACAAAATACCAATAACAGAAAAANNCTAACAAACTTCCTAACTTCTTCTAATCAAGATCCTCATCCATATAAATCTAATAGTATCTAACAGAAAGGAGGGTTTTTTTAATCCATTGCAATCTCCATCTTCAATCCTCTTCCACATCTTCATTTTTTCCTCTTCTTCATTCTTCACTCTCCAAATCTCTGCAACGATTCCAGATTCTTCTTCTTCTTCTTCCAACCAGTCATCACCGTAACAAACTTTCAACCTTTAAACAAAACCTTCAATCAAACGTCCATTTCCAGTCTACAACAACTCTACCAATCGCACCATCGTCCACCTCCTCTCCACATCAACCACTGCAAATCCAAACAGAAAACGGAGATCGATGTACAGCAACAGTAAATTCGAATCCAAACTCTGCGNNGNYNACCNYNTACTGCNNAGCGACGATAAGAAGAAGAAACACGGACGGTACCTGTTTTCGA
TARGET=253,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03566 Ps_contig_mira-and-tgicl-ass_franssen_30412
SEQUENCE=TANCAATTCTGAATTTTCATTTTATTTTATTTTTCTCAACTCTAAACGCACAAAACCCAGGTATCATATTGAAGATGTTAGAGCAGTTACTGATTTTCACAAGAGGCGGTTTAATTCTCTGGTCATGCAAAGAATTAGGTAACGCTTTGAAAGGTTCACCGATCGATACATTGATTCGATCATGTCTTCTCGAGGAGCGATCCGGTGCGGCTTCGTACAACTACGATGCACCTGGAGCTTCGTATTCGCTGAAATGGATCTTCCATAACGATCTCGGTTTGGTTTTCGTTGCGGTTTATCAGCGGATTCTGCATCTGTTGTATGTCGAGGATTTGTTAGCTGCTGTTAAGCGTGAATTCTCGCAGGTTTATGATCCTTCGCGAATGGTGTATCGGGATTTTGATGAGGTTTTTAAGCAGCTTAAGATTGAAGCTGAGGCGCGGGCTGAGGATTTGAAGAAATCGAATCCGGTGGTTGTGAATGGGAATAGGAAGAGTCAGGTTACGTGGAAGGGAGATGGATCTGAAGGGAAGAAGAATGGCGGTAGTGGCGGAGGTGGTGGTGGTTTGAAAAATGATGGTGATGGGAAAAATGGGATTAAANTCTGAGAGTGACCGTTTTGTTGTTGCTAATGGTAATAATAATAACGGTTCTAACTTGAAGAGTAATGGTGGTAATGTTAGTGTAAATGATAAAGATAATGATAGTTTGAATAATGGAGCTTTTGATGTAAATAGGCTTCAGAAGAAGGTTAGGAATAAAGGTGGGAATGGAAAGAAAACTGACAGTGTTGTATCCAAGGCTGAGCCGAAAAAATTGGTTAAGAAAAATAGGGTTTGGGATGAAAAACCCAAGGAAACTAAATTGGATTTTACCGATAAGGTGGATGTTGATCGGGATGCAGATTTGGATAGGAAAGTTGATTATTTGGCTAAGGAACAAGGGGAGAGTATGATGGATAAAGATGAGATTCTTAGTAGTGACAGTGAGGATGAAGAGGAGGATGGGGATGATGCTGGGAAGAATAGCAACAAGCCTGATGCTAAAAAGAAGGGTTGGTTTTCATCTATGTTTCAGAGTATTGCTGGGAAGGCCAATTTGGAGAAATCGGACTTGGAACCAGCTTTGAAAGCTCTGAAGGATAGACTCATGACCAAGAATGTGGCTGAAGAAATAGCTGAGAAGCTATGTGAATCAGTGGCAGCAAGTCTTGAAGGCAAAAAACTGGCTTCATTTACAAGGATATCTTCGACAGTGCATACGGCAATGGAAGAAGCACTTGTTCGTATTTTAACTCCTAGGCGTTCTATTGACATATTGAGGGATGTTCATGCTGCCAAAGAGCAAAGGAAACCATATGTTGTTGTATTTGTTGGAGTTAATGGAGTTGGAAAATCTACTAATCTTGCTAAGGTTGCTTACTGGCTTCAGCAACACAACGTTAATGTTATGATGGCTGCTTGTGATACATTTCGATCAGGAGCTGTTGAGCAGTTGCGGACTCATGCTCGTAGACTTCAGATCCCTATATTTGAGAAGGGTTATGAAAAGGATCCTGCTATTGTGGCAAAAGAAGCCATTCAGGAAGCTTCTCGTAATGGTTCGGATGTGGTTCTCGTCGATACAGCTGGTCGTATGCAGGATAATGAACCGTTGATGAGAGCACTGTCAAAGCTGATCTACCTTAACAATCCCGACCTGGTCTTATTTGTTGGAGAAGCACTTGTTGGCAATGATGCGGTTGATCAACTTTCAAAGTTCAACCAGAAACTGGCCGATCTCGCCTCATCTCCTACACCCAGATTGATAGATGGGATCTTGCTCACAAAGTTTGACACTATTGATGATAAGGTGGGAGCTGCACTTTCAATGGTTTACATATCTGGAGCTCCAGTCATGTTTGTGGGATGTGGACAGTCTTATACCGACCTCAAGAAACTCAACGTCAAGTCGATTGTGAAGACGCTTCTCAAATAAGTTGCAAAACTCTTCTCTTCACCTGTGATCTGTCACAAATGTTTTTAGTTTGCAATTACCTTGATGATCCTGGTTAGGCCAATACTCTGTAGGCCAAACTATGTGGCTATGACTTTGGATGCAGTTTGGTTTGTTTATTTTGGAAACTTTTCTTCCTCCCTTTATATCTATTTTGGAAAACTTAATGTATCCCTAAATAAAAATAGTTTGTTTGGGTTTCATTTCATATATGGTTTC
TARGET=19,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03567 Ps_contig_mira-and-tgicl-ass_franssen_30412
SEQUENCE=TANCAATTCTGAATTTTCATTTTATTTTATTTTTCTCAACTCTAAACGCACAAAACCCAGGTATCATATTGAAGATGTTAGAGCAGTTACTGATTTTCACAAGAGGCGGTTTAATTCTCTGGTCATGCAAAGAATTAGGTAACGCTTTGAAAGGTTCACCGATCGATACATTGATTCGATCATGTCTTCTCGAGGAGCGATCCGGTGCGGCTTCGTACAACTACGATGCACCTGGAGCTTCGTATTCGCTGAAATGGATCTTCCATAACGATCTCGGTTTGGTTTTCGTTGCGGTTTATCAGCGGATTCTGCATCTGTTGTATGTCGAGGATTTGTTAGCTGCTGTTAAGCGTGAATTCTCGCAGGTTTATGATCCTTCGCGAATGGTGTATCGGGATTTTGATGAGGTTTTTAAGCAGCTTAAGATTGAAGCTGAGGCGCGGGCTGAGGATTTGAAGAAATCGAATCCGGTGGTTGTGAATGGGAATAGGAAGAGTCAGGTTACGTGGAAGGGAGATGGATCTGAAGGGAAGAAGAATGGCGGTAGTGGCGGAGGTGGTGGTGGTTTGAAAAATGATGGTGATGGGAAAAATGGGATTAAANTCTGAGAGTGACCGTTTTGTTGTTGCTAATGGTAATAATAATAACGGTTCTAACTTGAAGAGTAATGGTGGTAATGTTAGTGTAAATGATAAAGATAATGATAGTTTGAATAATGGAGCTTTTGATGTAAATAGGCTTCAGAAGAAGGTTAGGAATAAAGGTGGGAATGGAAAGAAAACTGACAGTGTTGTATCCAAGGCTGAGCCGAAAAAATTGGTTAAGAAAAATAGGGTTTGGGATGAAAAACCCAAGGAAACTAAATTGGATTTTACCGATAAGGTGGATGTTGATCGGGATGCAGATTTGGATAGGAAAGTTGATTATTTGGCTAAGGAACAAGGGGAGAGTATGATGGATAAAGATGAGATTCTTAGTAGTGACAGTGAGGATGAAGAGGAGGATGGGGATGATGCTGGGAAGAATAGCAACAAGCCTGATGCTAAAAAGAAGGGTTGGTTTTCATCTATGTTTCAGAGTATTGCTGGGAAGGCCAATTTGGAGAAATCGGACTTGGAACCAGCTTTGAAAGCTCTGAAGGATAGACTCATGACCAAGAATGTGGCTGAAGAAATAGCTGAGAAGCTATGTGAATCAGTGGCAGCAAGTCTTGAAGGCAAAAAACTGGCTTCATTTACAAGGATATCTTCGACAGTGCATACGGCAATGGAAGAAGCACTTGTTCGTATTTTAACTCCTAGGCGTTCTATTGACATATTGAGGGATGTTCATGCTGCCAAAGAGCAAAGGAAACCATATGTTGTTGTATTTGTTGGAGTTAATGGAGTTGGAAAATCTACTAATCTTGCTAAGGTTGCTTACTGGCTTCAGCAACACAACGTTAATGTTATGATGGCTGCTTGTGATACATTTCGATCAGGAGCTGTTGAGCAGTTGCGGACTCATGCTCGTAGACTTCAGATCCCTATATTTGAGAAGGGTTATGAAAAGGATCCTGCTATTGTGGCAAAAGAAGCCATTCAGGAAGCTTCTCGTAATGGTTCGGATGTGGTTCTCGTCGATACAGCTGGTCGTATGCAGGATAATGAACCGTTGATGAGAGCACTGTCAAAGCTGATCTACCTTAACAATCCCGACCTGGTCTTATTTGTTGGAGAAGCACTTGTTGGCAATGATGCGGTTGATCAACTTTCAAAGTTCAACCAGAAACTGGCCGATCTCGCCTCATCTCCTACACCCAGATTGATAGATGGGATCTTGCTCACAAAGTTTGACACTATTGATGATAAGGTGGGAGCTGCACTTTCAATGGTTTACATATCTGGAGCTCCAGTCATGTTTGTGGGATGTGGACAGTCTTATACCGACCTCAAGAAACTCAACGTCAAGTCGATTGTGAAGACGCTTCTCAAATAAGTTGCAAAACTCTTCTCTTCACCTGTGATCTGTCACAAATGTTTTTAGTTTGCAATTACCTTGATGATCCTGGTTAGGCCAATACTCTGTAGGCCAAACTATGTGGCTATGACTTTGGATGCAGTTTGGTTTGTTTATTTTGGAAACTTTTCTTCCTCCCTTTATATCTATTTTGGAAAACTTAATGTATCCCTAAATAAAAATAGTTTGTTTGGGTTTCATTTCATATATGGTTTC
TARGET=555,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2416
PRIMER_LEFT_PENALTY=0.167063
PRIMER_RIGHT_PENALTY=0.074581
PRIMER_LEFT_SEQUENCE=AGGAAGAGTCAGGTTACGTGGA
PRIMER_RIGHT_SEQUENCE=TTAGCAACAACAAAACGGTCAC
PRIMER_LEFT=488,22
PRIMER_RIGHT=631,22
PRIMER_LEFT_TM=60.167
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=0.3414
PRIMER_LEFT_1_PENALTY=0.266783
PRIMER_RIGHT_1_PENALTY=0.074581
PRIMER_LEFT_1_SEQUENCE=GTTAGCTGCTGTTAAGCGTGAA
PRIMER_RIGHT_1_SEQUENCE=TTAGCAACAACAAAACGGTCAC
PRIMER_LEFT_1=334,22
PRIMER_RIGHT_1=631,22
PRIMER_LEFT_1_TM=59.733
PRIMER_RIGHT_1_TM=60.075
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.3708
PRIMER_LEFT_2_PENALTY=0.296177
PRIMER_RIGHT_2_PENALTY=0.074581
PRIMER_LEFT_2_SEQUENCE=GGTTGTGAATGGGAATAGGAAG
PRIMER_RIGHT_2_SEQUENCE=TTAGCAACAACAAAACGGTCAC
PRIMER_LEFT_2=472,22
PRIMER_RIGHT_2=631,22
PRIMER_LEFT_2_TM=59.704
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=0.3823
PRIMER_LEFT_3_PENALTY=0.167063
PRIMER_RIGHT_3_PENALTY=0.215198
PRIMER_LEFT_3_SEQUENCE=AGGAAGAGTCAGGTTACGTGGA
PRIMER_RIGHT_3_SEQUENCE=AGTTTTCTTTCCATTCCCACCT
PRIMER_LEFT_3=488,22
PRIMER_RIGHT_3=782,22
PRIMER_LEFT_3_TM=60.167
PRIMER_RIGHT_3_TM=60.215
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=0.3978
PRIMER_LEFT_4_PENALTY=0.323242
PRIMER_RIGHT_4_PENALTY=0.074581
PRIMER_LEFT_4_SEQUENCE=AAGAGTCAGGTTACGTGGAAGG
PRIMER_RIGHT_4_SEQUENCE=TTAGCAACAACAAAACGGTCAC
PRIMER_LEFT_4=491,22
PRIMER_RIGHT_4=631,22
PRIMER_LEFT_4_TM=59.677
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_03568 Ps_contig_mira-and-tgicl-ass_franssen_30412
SEQUENCE=TANCAATTCTGAATTTTCATTTTATTTTATTTTTCTCAACTCTAAACGCACAAAACCCAGGTATCATATTGAAGATGTTAGAGCAGTTACTGATTTTCACAAGAGGCGGTTTAATTCTCTGGTCATGCAAAGAATTAGGTAACGCTTTGAAAGGTTCACCGATCGATACATTGATTCGATCATGTCTTCTCGAGGAGCGATCCGGTGCGGCTTCGTACAACTACGATGCACCTGGAGCTTCGTATTCGCTGAAATGGATCTTCCATAACGATCTCGGTTTGGTTTTCGTTGCGGTTTATCAGCGGATTCTGCATCTGTTGTATGTCGAGGATTTGTTAGCTGCTGTTAAGCGTGAATTCTCGCAGGTTTATGATCCTTCGCGAATGGTGTATCGGGATTTTGATGAGGTTTTTAAGCAGCTTAAGATTGAAGCTGAGGCGCGGGCTGAGGATTTGAAGAAATCGAATCCGGTGGTTGTGAATGGGAATAGGAAGAGTCAGGTTACGTGGAAGGGAGATGGATCTGAAGGGAAGAAGAATGGCGGTAGTGGCGGAGGTGGTGGTGGTTTGAAAAATGATGGTGATGGGAAAAATGGGATTAAANTCTGAGAGTGACCGTTTTGTTGTTGCTAATGGTAATAATAATAACGGTTCTAACTTGAAGAGTAATGGTGGTAATGTTAGTGTAAATGATAAAGATAATGATAGTTTGAATAATGGAGCTTTTGATGTAAATAGGCTTCAGAAGAAGGTTAGGAATAAAGGTGGGAATGGAAAGAAAACTGACAGTGTTGTATCCAAGGCTGAGCCGAAAAAATTGGTTAAGAAAAATAGGGTTTGGGATGAAAAACCCAAGGAAACTAAATTGGATTTTACCGATAAGGTGGATGTTGATCGGGATGCAGATTTGGATAGGAAAGTTGATTATTTGGCTAAGGAACAAGGGGAGAGTATGATGGATAAAGATGAGATTCTTAGTAGTGACAGTGAGGATGAAGAGGAGGATGGGGATGATGCTGGGAAGAATAGCAACAAGCCTGATGCTAAAAAGAAGGGTTGGTTTTCATCTATGTTTCAGAGTATTGCTGGGAAGGCCAATTTGGAGAAATCGGACTTGGAACCAGCTTTGAAAGCTCTGAAGGATAGACTCATGACCAAGAATGTGGCTGAAGAAATAGCTGAGAAGCTATGTGAATCAGTGGCAGCAAGTCTTGAAGGCAAAAAACTGGCTTCATTTACAAGGATATCTTCGACAGTGCATACGGCAATGGAAGAAGCACTTGTTCGTATTTTAACTCCTAGGCGTTCTATTGACATATTGAGGGATGTTCATGCTGCCAAAGAGCAAAGGAAACCATATGTTGTTGTATTTGTTGGAGTTAATGGAGTTGGAAAATCTACTAATCTTGCTAAGGTTGCTTACTGGCTTCAGCAACACAACGTTAATGTTATGATGGCTGCTTGTGATACATTTCGATCAGGAGCTGTTGAGCAGTTGCGGACTCATGCTCGTAGACTTCAGATCCCTATATTTGAGAAGGGTTATGAAAAGGATCCTGCTATTGTGGCAAAAGAAGCCATTCAGGAAGCTTCTCGTAATGGTTCGGATGTGGTTCTCGTCGATACAGCTGGTCGTATGCAGGATAATGAACCGTTGATGAGAGCACTGTCAAAGCTGATCTACCTTAACAATCCCGACCTGGTCTTATTTGTTGGAGAAGCACTTGTTGGCAATGATGCGGTTGATCAACTTTCAAAGTTCAACCAGAAACTGGCCGATCTCGCCTCATCTCCTACACCCAGATTGATAGATGGGATCTTGCTCACAAAGTTTGACACTATTGATGATAAGGTGGGAGCTGCACTTTCAATGGTTTACATATCTGGAGCTCCAGTCATGTTTGTGGGATGTGGACAGTCTTATACCGACCTCAAGAAACTCAACGTCAAGTCGATTGTGAAGACGCTTCTCAAATAAGTTGCAAAACTCTTCTCTTCACCTGTGATCTGTCACAAATGTTTTTAGTTTGCAATTACCTTGATGATCCTGGTTAGGCCAATACTCTGTAGGCCAAACTATGTGGCTATGACTTTGGATGCAGTTTGGTTTGTTTATTTTGGAAACTTTTCTTCCTCCCTTTATATCTATTTTGGAAAACTTAATGTATCCCTAAATAAAAATAGTTTGTTTGGGTTTCATTTCATATATGGTTTC
TARGET=636,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1436
PRIMER_LEFT_PENALTY=0.074581
PRIMER_RIGHT_PENALTY=0.069038
PRIMER_LEFT_SEQUENCE=GTGACCGTTTTGTTGTTGCTAA
PRIMER_RIGHT_SEQUENCE=CCACCTTATCGGTAAAATCCAA
PRIMER_LEFT=610,22
PRIMER_RIGHT=885,22
PRIMER_LEFT_TM=60.075
PRIMER_RIGHT_TM=60.069
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.1560
PRIMER_LEFT_1_PENALTY=0.074581
PRIMER_RIGHT_1_PENALTY=0.081468
PRIMER_LEFT_1_SEQUENCE=GTGACCGTTTTGTTGTTGCTAA
PRIMER_RIGHT_1_SEQUENCE=GATCAACATCCACCTTATCGGT
PRIMER_LEFT_1=610,22
PRIMER_RIGHT_1=894,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.2898
PRIMER_LEFT_2_PENALTY=0.074581
PRIMER_RIGHT_2_PENALTY=0.215198
PRIMER_LEFT_2_SEQUENCE=GTGACCGTTTTGTTGTTGCTAA
PRIMER_RIGHT_2_SEQUENCE=AGTTTTCTTTCCATTCCCACCT
PRIMER_LEFT_2=610,22
PRIMER_RIGHT_2=782,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=60.215
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.3537
PRIMER_LEFT_3_PENALTY=0.284619
PRIMER_RIGHT_3_PENALTY=0.069038
PRIMER_LEFT_3_SEQUENCE=AGTGACCGTTTTGTTGTTGCTA
PRIMER_RIGHT_3_SEQUENCE=CCACCTTATCGGTAAAATCCAA
PRIMER_LEFT_3=609,22
PRIMER_RIGHT_3=885,22
PRIMER_LEFT_3_TM=59.715
PRIMER_RIGHT_3_TM=60.069
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.3581
PRIMER_LEFT_4_PENALTY=0.074581
PRIMER_RIGHT_4_PENALTY=0.283555
PRIMER_LEFT_4_SEQUENCE=GTGACCGTTTTGTTGTTGCTAA
PRIMER_RIGHT_4_SEQUENCE=GGTTTTTCATCCCAAACCCTAT
PRIMER_LEFT_4=610,22
PRIMER_RIGHT_4=850,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=60.284
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_03569 Ps_contig_mira-and-tgicl-ass_franssen_30418
SEQUENCE=AAACTTTAAAAAACAAATATTCATAAATATTCTTATCATTAAATCTTCCAAACAACATATTACAAACTCAGAATCAAACAACCAACTAGAAGCACCCATAATAATAATAACAATGGTTCCACACAAGTAATCAAACAAACAAACAAGGAAGAAAAAAACACCACACCACGCCGACAGCAACAAAAGTCTCACATCCCCTACGGAAAAAACACCGAAAGTTACGTCCCGTGAAAACGTGAAACAAAAGCCAACCACCAACCGCCACGTGTACAGGACTTTATTTTATTCTAATCCACTCACCTAAACACGTGATCGGGTCTTAATTTTATTTTAACCTTTTTTTATATTGCGAGAACCAAACCGGGTACGGATTTACCGACCCGATCAGAAACGCTACTCTCCCAGACGCAAGGGAAGCGGCCGGAATCGAACGTCGCCGGGACAATCTCCTCGGAGTCTTTCTCGGCGAAGCGAGTGACGGTGAGGGAAGTGTTGAAATACTCCCTAACTTGGGTGATTATCCCATCCGCTACGGTCAGCGCGTGAACCCAGGAAATGGCGCGTGAAGTATCGCAGCCTTCGACGATGATGGTGGAACCGAAAGAGACGAGTGACTGTGGAACGAAACGGAAGGTATCGACGGCGGTGGAATCGCCGGTGAGCATGCGCATCAAAAACTGATGAGAGGGTGGACCGTGAAACCACCACTCAAGATCAGAAGCGAGAATCTTGACCACGGTGTCGGAATCTCGAGAGTTTAAGGCTTCATATAGTGTGAGAACCAGCTTTTGATGGCTGGAATCAGCTTCTTCGCCACTTTCTTGAAAGTTAGCCAGTTCGAGAAAAAGATGTTCTTCTAGAAATTTCGATTTAGGGAGATGGAAATGGAATGTTGAGAAATGGTTATTTTTTTTTTTGNGATGTTGTTGCTTTGTTCCCCGNGTACTCTGCGTT
TARGET=98,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5222
PRIMER_LEFT_PENALTY=0.435201
PRIMER_RIGHT_PENALTY=0.087031
PRIMER_LEFT_SEQUENCE=AAACAACCAACTAGAAGCACCC
PRIMER_RIGHT_SEQUENCE=GTTTTCACGGGACGTAACTTTC
PRIMER_LEFT=75,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=59.565
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=0.6551
PRIMER_LEFT_1_PENALTY=0.435201
PRIMER_RIGHT_1_PENALTY=0.219884
PRIMER_LEFT_1_SEQUENCE=AAACAACCAACTAGAAGCACCC
PRIMER_RIGHT_1_SEQUENCE=GGATGTGAGACTTTTGTTGCTG
PRIMER_LEFT_1=75,22
PRIMER_RIGHT_1=195,22
PRIMER_LEFT_1_TM=59.565
PRIMER_RIGHT_1_TM=59.780
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=121
PRIMER_PAIR_PENALTY_2=0.9153
PRIMER_LEFT_2_PENALTY=0.435201
PRIMER_RIGHT_2_PENALTY=0.480050
PRIMER_LEFT_2_SEQUENCE=AAACAACCAACTAGAAGCACCC
PRIMER_RIGHT_2_SEQUENCE=ATCACGTGTTTAGGTGAGTGGA
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=312,22
PRIMER_LEFT_2_TM=59.565
PRIMER_RIGHT_2_TM=59.520
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.9153
PRIMER_LEFT_3_PENALTY=0.435201
PRIMER_RIGHT_3_PENALTY=0.480050
PRIMER_LEFT_3_SEQUENCE=AAACAACCAACTAGAAGCACCC
PRIMER_RIGHT_3_SEQUENCE=GATCACGTGTTTAGGTGAGTGG
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=313,22
PRIMER_LEFT_3_TM=59.565
PRIMER_RIGHT_3_TM=59.520
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=0.9153
PRIMER_LEFT_4_PENALTY=0.435201
PRIMER_RIGHT_4_PENALTY=0.480050
PRIMER_LEFT_4_SEQUENCE=AAACAACCAACTAGAAGCACCC
PRIMER_RIGHT_4_SEQUENCE=TCACGTGTTTAGGTGAGTGGAT
PRIMER_LEFT_4=75,22
PRIMER_RIGHT_4=311,22
PRIMER_LEFT_4_TM=59.565
PRIMER_RIGHT_4_TM=59.520
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_03570 Ps_contig_mira-and-tgicl-ass_franssen_30418
SEQUENCE=AAACTTTAAAAAACAAATATTCATAAATATTCTTATCATTAAATCTTCCAAACAACATATTACAAACTCAGAATCAAACAACCAACTAGAAGCACCCATAATAATAATAACAATGGTTCCACACAAGTAATCAAACAAACAAACAAGGAAGAAAAAAACACCACACCACGCCGACAGCAACAAAAGTCTCACATCCCCTACGGAAAAAACACCGAAAGTTACGTCCCGTGAAAACGTGAAACAAAAGCCAACCACCAACCGCCACGTGTACAGGACTTTATTTTATTCTAATCCACTCACCTAAACACGTGATCGGGTCTTAATTTTATTTTAACCTTTTTTTATATTGCGAGAACCAAACCGGGTACGGATTTACCGACCCGATCAGAAACGCTACTCTCCCAGACGCAAGGGAAGCGGCCGGAATCGAACGTCGCCGGGACAATCTCCTCGGAGTCTTTCTCGGCGAAGCGAGTGACGGTGAGGGAAGTGTTGAAATACTCCCTAACTTGGGTGATTATCCCATCCGCTACGGTCAGCGCGTGAACCCAGGAAATGGCGCGTGAAGTATCGCAGCCTTCGACGATGATGGTGGAACCGAAAGAGACGAGTGACTGTGGAACGAAACGGAAGGTATCGACGGCGGTGGAATCGCCGGTGAGCATGCGCATCAAAAACTGATGAGAGGGTGGACCGTGAAACCACCACTCAAGATCAGAAGCGAGAATCTTGACCACGGTGTCGGAATCTCGAGAGTTTAAGGCTTCATATAGTGTGAGAACCAGCTTTTGATGGCTGGAATCAGCTTCTTCGCCACTTTCTTGAAAGTTAGCCAGTTCGAGAAAAAGATGTTCTTCTAGAAATTTCGATTTAGGGAGATGGAAATGGAATGTTGAGAAATGGTTATTTTTTTTTTTGNGATGTTGTTGCTTTGTTCCCCGNGTACTCTGCGTT
TARGET=132,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1587
PRIMER_LEFT_PENALTY=0.071684
PRIMER_RIGHT_PENALTY=0.087031
PRIMER_LEFT_SEQUENCE=ACAACCAACTAGAAGCACCCAT
PRIMER_RIGHT_SEQUENCE=GTTTTCACGGGACGTAACTTTC
PRIMER_LEFT=77,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=0.5222
PRIMER_LEFT_1_PENALTY=0.435201
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=AAACAACCAACTAGAAGCACCC
PRIMER_RIGHT_1_SEQUENCE=GTTTTCACGGGACGTAACTTTC
PRIMER_LEFT_1=75,22
PRIMER_RIGHT_1=234,22
PRIMER_LEFT_1_TM=59.565
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.5517
PRIMER_LEFT_2_PENALTY=0.071684
PRIMER_RIGHT_2_PENALTY=0.480050
PRIMER_LEFT_2_SEQUENCE=ACAACCAACTAGAAGCACCCAT
PRIMER_RIGHT_2_SEQUENCE=ATCACGTGTTTAGGTGAGTGGA
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=312,22
PRIMER_LEFT_2_TM=59.928
PRIMER_RIGHT_2_TM=59.520
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.5517
PRIMER_LEFT_3_PENALTY=0.071684
PRIMER_RIGHT_3_PENALTY=0.480050
PRIMER_LEFT_3_SEQUENCE=ACAACCAACTAGAAGCACCCAT
PRIMER_RIGHT_3_SEQUENCE=GATCACGTGTTTAGGTGAGTGG
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=313,22
PRIMER_LEFT_3_TM=59.928
PRIMER_RIGHT_3_TM=59.520
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=0.5517
PRIMER_LEFT_4_PENALTY=0.071684
PRIMER_RIGHT_4_PENALTY=0.480050
PRIMER_LEFT_4_SEQUENCE=ACAACCAACTAGAAGCACCCAT
PRIMER_RIGHT_4_SEQUENCE=TCACGTGTTTAGGTGAGTGGAT
PRIMER_LEFT_4=77,22
PRIMER_RIGHT_4=311,22
PRIMER_LEFT_4_TM=59.928
PRIMER_RIGHT_4_TM=59.520
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_03571 Ps_contig_mira-and-tgicl-ass_franssen_30419
SEQUENCE=AGTTTTTTACCTTAAAACTNGTGCAATTACTTTTACTTTTTTTCTATGYCTAAATAGTTAATAAACATGATTTATAATACTGTGGGAAATAATTGACATATATCTATATTTGTAAGAAACTGCAAAGGCAAACGTTTTCGAGTGTTTATCTATATTTGATGTTTTTAATAAGTGTAATGGAAACATATGCTACTAAAGATGAAGAACAGTAATGTTACTATCTACCTCCATAAAGACGCCACTGCGACAATTTATTTGGTTCAAAGTAGCTGGCAAACTATATTAAAGAAAAGCTATTAGACTGATGCATCTCTCTACCTCTTGCTCTAAACATACATACATCATTGATGATCCCCTCTTACCATTGCATTTGAGACAGCACTTCCTCCTTAGTACTTGATTAGAATGCATCGTATTGGTGATCCCTCAGCACCAGCCAACCTAAGAGGTAAGCAGTGGACTGAGTATAATCCTGCTGGGACATCATCAAGTTTCAGGCTTTCAACAAGAATGATTTCCCTGTCTTTTAAAAACACAAGGTGGGATGGAATCAAGTAATCATAAGAAGCAACAGATAGGTAGTCAATACCAACAAGTTTGATATCGGTGTTTTCCACTAGCCATTTTGCTCCATCTACAGTGAACCCCACATAGTTTGAGTCAAATTCCTTCTGAAACATAAGCCGCCTGTCGGTATTTAATGTTCGGAAGAGTACACGCTTTACACCCCTGGGGATATTTAATGACTTCAGAACTTCAGCAGTTATATTACTATTTCTTGGAACATCAACTAAAAGAGCAGGACCATTGAGGACGTGCAAGTCGAGTGAATCAACATCAAATCCAGCATCGAAGTAGTGATCGTAGACGTGGCCAGGTGCATCAACATGAGTTCCGGTATGAGCCGGAAGTTTCATTTCAGAGTTATTAGCGATGGAACCGTTTTTCATGCTCTTTGGAAGCCACAGGAATTGACCGATACCGTTTTTAGATTCAAACTCTGGCATGTCCGGGTGATACCTGTGAGTGATATCGAAGATTCTTCCGTTATCGTAAAGTTCGCGGCGAGGAGGGATGAGGTTTTCGTCGTCGATTACCGTCGACGTGCAAATTGCCGCCATGAAAGCGAGCGCTACCGCTTGGTAGTTCATTTTGGATTTGTTGGTTTTGGTGATGTGCACAGTACAAAAGAGAAAAGCTTTTCTTTCTCTTGTCTTCCTTGTGTTTTCTGCTGT
TARGET=331,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03572 Ps_contig_mira-and-tgicl-ass_franssen_30420
SEQUENCE=ATTGGGTTTNGATTTGGTGCGGTGTTGTTGTATTGATTTGAAGCGTGCGTATGCATTCGTATCGGTGACAGAAGAAGAAGAAGAAGAAGAGAAAGAGAGTGAAATGGGAGAATCAAGGGAAGAGAAAATTGAAGAATCCGTGGCTTCCGTTTTGGAAGAAGCTAAAGAGCTTCAAGATTCCGTCGCCGGTCACATCTCCAAATCGTTAAGCGACGAACATCCTCTCCGGCAACGAGCTCTTGCTCTCGACTCCAAAATCCTTTCACTTCGTTCTTCTCTCGATTCTCTCCTCAACAATAAACTAATTAATCACTCCGTAGCTGATAAGTTAGATGAAGATTTGCAGAGAGCGAGATGTATTGTGGTTGATGGTGATGCTTCTTCGCTTCTTCCAGGACATGCTCACGGGAAGTTTCTGAGGATGTTTTTGGGTCCTATTAATGTTCGTGCATCTCGGAAAGATGTGCAGCTTAAGGTCAAAGAGGAATACAACAGTTATAGGGATAGAACTGCTCTGCTATTCCTGCTTTTCCCGGCAGCACTTCTCATTCTAAGATCATGGGTTTGGGATGGATGCTTGCCAGCTTTTCCCGTACAGATTTACCAGGCCTGGTTGCTATTTCTTTATACTGGTTTGGCATTGCGAGAGAACATATTGAGAGTAAATGGAAGTGATATCCGTCCATGGTGGATATATCATCATTATTGTGCCATGATAATGGCCGTTGTGAGTCTCACTTGGGAAATAAAAGGACAACCAGACTGCGCAAATAAGCAGAGAGGTGTACAGCTGTTCCTACAGTGGGCTATGATGCAAGGAGTTGCAATGCTTTTGCAAAATAGATATCAACGACAGAGACTTTATACTCGTATTGCATTGGGAAAGGCGAAGAGGATGGATGTTGTATGGGGGGAAACATCTGGTGTGGATGGCCAACTGTGGTTGCTGTGTCCCATACTTTTCATATTGCAGGGATTTGAGGCATATGTTGGACTATTGTTGCTCAATACTGCATTTGCTGGGGAGATTTCTGAATGGCAGGTAATATTTTGCGGTCTTCTGTTGGTCGTAATGGCCATTGGGAACTTTGAAAATACAGTACAGACTCTGTTGGTAAAGTCAAGATTTAAGGCAAAAATGAGAAGAACCAAGAGCAAGCAGAGACTGGATTAGCAAGTTGTGACATCTTGAGCTTTTGATAGGATCAGAATCTTTTTGCAGTCTTTTAAGTTTTGACCACTGTGCATGTCTAGTGATAATTTTGGCTTTGTTGTAGCATCATACATTTAACGCGTCTAAAGCGTAAGTTTGGATTTTATCTCTATAAGTGCATTGTTTTACAACTTTGTACACTCTTTTGGGTCGGCGTACTCATTCCCTGACGTGGTAGATTGATGATTTTCCTCTCTTCCACACTATATTTTAGATTATTCAAAGTGATTTGCAGAT
TARGET=70,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1758
PRIMER_LEFT_PENALTY=0.156983
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=GTATTGATTTGAAGCGTGCGTA
PRIMER_RIGHT_SEQUENCE=ATTTTGGAGTCGAGAGCAAGAG
PRIMER_LEFT=29,22
PRIMER_RIGHT=257,22
PRIMER_LEFT_TM=60.157
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.1768
PRIMER_LEFT_1_PENALTY=0.156983
PRIMER_RIGHT_1_PENALTY=0.019805
PRIMER_LEFT_1_SEQUENCE=GTATTGATTTGAAGCGTGCGTA
PRIMER_RIGHT_1_SEQUENCE=GAACGAAGTGAAAGGATTTTGG
PRIMER_LEFT_1=29,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=60.157
PRIMER_RIGHT_1_TM=59.980
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.9000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.2119
PRIMER_LEFT_2_PENALTY=0.156983
PRIMER_RIGHT_2_PENALTY=0.054900
PRIMER_LEFT_2_SEQUENCE=GTATTGATTTGAAGCGTGCGTA
PRIMER_RIGHT_2_SEQUENCE=CCACGGATTCTTCAATTTTCTC
PRIMER_LEFT_2=29,22
PRIMER_RIGHT_2=142,22
PRIMER_LEFT_2_TM=60.157
PRIMER_RIGHT_2_TM=59.945
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=0.2221
PRIMER_LEFT_3_PENALTY=0.203283
PRIMER_RIGHT_3_PENALTY=0.018835
PRIMER_LEFT_3_SEQUENCE=GTGCGGTGTTGTTGTATTGATT
PRIMER_RIGHT_3_SEQUENCE=ATTTTGGAGTCGAGAGCAAGAG
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=59.797
PRIMER_RIGHT_3_TM=60.019
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.2231
PRIMER_LEFT_4_PENALTY=0.203283
PRIMER_RIGHT_4_PENALTY=0.019805
PRIMER_LEFT_4_SEQUENCE=GTGCGGTGTTGTTGTATTGATT
PRIMER_RIGHT_4_SEQUENCE=GAACGAAGTGAAAGGATTTTGG
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=272,22
PRIMER_LEFT_4_TM=59.797
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_03573 Ps_contig_mira-and-tgicl-ass_franssen_30426
SEQUENCE=AAGTTTGTGATTTACATTANATTCCATATATCCTTTTTATCTAACTTAACTACTCACAAACACATACAAGCACTAGTTTTCAGAATTCGATCAAGATTACACCACTACACACCACATCAATGGCATCACTAACCATGACAGCACCATTCTTCGCCGGCAGCTCAGCGATCGCCACCCGGTCTCCCGTGGCAACTCAAAGGAAACTGGTGGTGGCTAATGCTGCTAGAGGAGTTGAAGTAGAAAAGATGAAAGTGAGTTATGAGAATGAAAAGGAAGGAACCAATGGAAGGAGGAACATCATGTTTGCTGCTGCTGCAGCAGCTGTTTGTTCTGTTGCTGGGATGGCTTTGGCTGATGAACCTAAACGTGGTTCTCCAGAAGCTAAGAAAGCATATGCACCTGTTTGTGTTACCATGCCAACAGCTAGGATTTGTCGCAACTGAGGGTGTTTTAGTCTTTTATGTACTTGTATTGTAATGAAATGAACATTGGGGATGCAATGTTTTGGTTTCATATTCTTATCCTTTGTGATTCTGTGTACTAAGTTGTTCATGAAACTATCAAATTTGCCTTGTCTATGTTATTGTTAGTTTGATGCTCAACCAAGAANTTTTGGTTAAGTAAGAAA
TARGET=305,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1129
PRIMER_LEFT_PENALTY=0.079714
PRIMER_RIGHT_PENALTY=0.033137
PRIMER_LEFT_SEQUENCE=CACTAACCATGACAGCACCATT
PRIMER_RIGHT_SEQUENCE=TCTGGAGAACCACGTTTAGGTT
PRIMER_LEFT=126,22
PRIMER_RIGHT=378,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.1129
PRIMER_LEFT_1_PENALTY=0.079714
PRIMER_RIGHT_1_PENALTY=0.033137
PRIMER_LEFT_1_SEQUENCE=CACTAACCATGACAGCACCATT
PRIMER_RIGHT_1_SEQUENCE=TTCTGGAGAACCACGTTTAGGT
PRIMER_LEFT_1=126,22
PRIMER_RIGHT_1=379,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=60.033
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=254
PRIMER_PAIR_PENALTY_2=0.1513
PRIMER_LEFT_2_PENALTY=0.118120
PRIMER_RIGHT_2_PENALTY=0.033137
PRIMER_LEFT_2_SEQUENCE=ATGGCATCACTAACCATGACAG
PRIMER_RIGHT_2_SEQUENCE=TCTGGAGAACCACGTTTAGGTT
PRIMER_LEFT_2=119,22
PRIMER_RIGHT_2=378,22
PRIMER_LEFT_2_TM=59.882
PRIMER_RIGHT_2_TM=60.033
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.1513
PRIMER_LEFT_3_PENALTY=0.118120
PRIMER_RIGHT_3_PENALTY=0.033137
PRIMER_LEFT_3_SEQUENCE=ATGGCATCACTAACCATGACAG
PRIMER_RIGHT_3_SEQUENCE=TTCTGGAGAACCACGTTTAGGT
PRIMER_LEFT_3=119,22
PRIMER_RIGHT_3=379,22
PRIMER_LEFT_3_TM=59.882
PRIMER_RIGHT_3_TM=60.033
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.2371
PRIMER_LEFT_4_PENALTY=0.079714
PRIMER_RIGHT_4_PENALTY=0.157415
PRIMER_LEFT_4_SEQUENCE=CACTAACCATGACAGCACCATT
PRIMER_RIGHT_4_SEQUENCE=CTTCTGGAGAACCACGTTTAGG
PRIMER_LEFT_4=126,22
PRIMER_RIGHT_4=380,22
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=60.157
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_03574 Ps_contig_mira-and-tgicl-ass_franssen_30429
SEQUENCE=AAANTTTTNAAGTGTTYCAACTTTGGGTAGTAGATTATACTCAAATATTGTCCAAAAGTAAACAAGAACAAGGGCGACAATACATGCATTCTTGAATACCTTCAATTTAAAAAGAAATGTTTGAATGTCCGTACGGTTTCTTAAAAGTCGATCTTGTTTTAACGTATTGTTCTGAAAGGGGAAAATGCGTTTTTCTCTATGTCCACTCATCCGGATGTAGAAAAACCCGTGTTTTTACCTATCAGACCAATACGTTACAACAAGATCGATTTTTAAGAAACCGTGCGGAAGAAAAAAAACTCTCTAATTTGAATTAAGTCTAGTCACTTGACCTTCCTTTTGCCCATAAGCCCAAGAACTTCATCCCAAAGCCCAACAATTCCAACATTCCCCAAATCAACAACACTTGGACTACAAGCAGCCACAGTCCAATAACCAGCCATAATTTCATCTTTCAATTGTTCTTTCTCCCACCCACAATACCCATCAAAGAATCTAAAATCACCAACCTCAACCACATTCCTCTTCACCATTTCAGAACCACATCCTACACTTTCCTTTGTACCATAATACAAACCCTTCATTACTTCATCAAAAACACCACTTATTCTCACCACACCATCATCTTTAGGACTCACCAAAAACAACCCTTCTTCTAAAGGTCCTCCAAAATACAAAGGACTATTAGAAAATGTACCCTTTACATCAAATGCTGTTGATCTTGTTTCTTTAATAGACATCAATGATGGTCTATTGAGTATGATCCCGGATGGGCCTATTGGACCGTTTGATAAAAGAAGGATCACCGTCCGTTCGAAAATGTGGACCCCATCGAGCTTTTCTGTTGCGATTAACAAACAGCCTCTTTCGGGCTCATGGATTATATGGGCCCATTTGTCACCAATTGTGATTAGTGATGGTTGATCCATAACGGTGTCCGGATTAGTCACTGATGAAAGTAACTCCGGGTTTAGTAATTGTTCTCCGGCCACCAATTTTGCCCGGAAAGTTCGCCAATCGGTATTAACAGTTGGTTTGTCATCGTCTAACGGCGATGATGATGATGATGATGACATGTGGCAACATGATACATGAAATGGACTTGCAACTTTTCTTGAATTGAATTGTTGAGAAGATCTTTTGAAGTATGTTAGAGATCCAGCTCTGATTTTGGTGAAGGAAGCTTGGGAGAGAAAACAAGCATCCATGTTATTTGAGTACTATTTTCTTGTTTAGATGAGAGT
TARGET=1054,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03575 Ps_contig_mira-and-tgicl-ass_franssen_30431
SEQUENCE=AAAAAGAAACAAAAGTAAAGGTAAAATAAACAAAACGRNCATNGNNGAAATCTCGAACCATCTTCGTTTCCATCTCCATCTCTCTCTCTCTCTCTCTGCAACATTGAGATCGCTTTCGCCAATTTCAATGATACACTTTGTGCTGCTCATTAGTCGTCAAGGGAAGGTCAGGCTCACAAAATGGTATTCCCCATACACTCAGAAGGAAAGAAGTAAGGTAATCCGTGAGCTCAGTGGAGTGATTCTTAGCCGTGCTCCCAAGCTATGTAACTTTGTAGAGTGGAGAGGACATAAAGTTGTTTATAAAAGGTATGCTAGTCTTTATTTCTGTATGTGTATTGATGATGCTGACAACGAATTGGAAGTCCTTGAAATGATTCATCATTTTGTGGAGATTCTGGATCGGTATTTTGGCAGTGTATGTGAATTGGACTTGATATTCAACTTCCATAAGGCCTATTATATACTTGATGAACTTTTAATTGCTGGTGAGCTTCAGGAGTCAAGCAAAAAAACTGTTGCCCGGTTGATAGCTGCACAGGATTCATTGGTGGAGACTGCCAAGGAAGAAGCCAGTTCCTTGAGTAATATTATCGCACAAGCCACGAAGTAAGGATATCCGATGTTGTAATTGTTGGGATTTCTAGGAATGCAGAAAAGTTTGGTGCAATTGAAGTGTGAACTTACGGTTTATGTCTATGTTCCATCTTTATTTTTAAGTTGAATTTGTAAAATAGAAAAGAAAGAGAAAAAACAAGTCTTGACATGAACATTGATCTGCGTTGCAATTCCCATGGATGGCTATTTCAAGTTGGTATACATGTGTTAACCTTTTTATTACCCGTGTTGATACATGTGTCTTTGAAATTTGTTAATTTTTTAATNTTGTTA
TARGET=79,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03576 Ps_contig_mira-and-tgicl-ass_franssen_30432
SEQUENCE=GTCGGGGGGGGTTTGTCGGTGGGGGTTGGGTTTCTCACTTTCTCTTCATTCATCTGTTTCAACACTCCAAAAAATATATTATTATTATTTATTTATATCCGTTTCGTCTTCCCCTCTCTCTGAATTCTCAGATCAAACCATCTTCTTCATCAGGAAGCTTGGAGACTTCACACTCTCGTGTTTCAATCCACACAATACAAAATGTTTTTCGAGGGATATGGATATCACGGGACATCGTTTGAGCAGACATATCGATGCTACCCTGCTTCTTTTATTGAAAAGCCCCAACTTGAAAGTGGTGACAAAATTATAATGCCGCCATCAGCCCTTGATCGTCTAGCATCTCTGCATATTGATTATCCAATGTTGTTTGAACTTCGTAATGATGCTGCTGAGCGAGTTTCTCATTGTGGTGTTCTGGAATTCATTGCAGAGGAAGGCATGATATACATGCCATACTGGATGATGGAGAACATGCTTTTACAAGAGGGGGACATTGTAAGAGTGAAAAATGCGACACTTCCAAAGGGAACATATGTTAAGTTACAGCCTCACACAAAAGACTTTTTGGATATTTCCAATCCAAAGGCTATATTAGAAACTACTTTGAGGAATTTTTCCTGCTTGACTACTGGAGATAGCATTATGGTGGCTTACAATAACAAAAAGTATTATATTGATATTATAGAAAGCAAGCCTGCCAATGCTATAAGCATCATTGAGACTGATTGTGAAGTGGACTTTGCCCCTCCCTTAGATTACAAGGAACCTGAAAAGCCCGTTCCTTCACGTTCTGCTGGAAAGGCACCAGAAGCTGATAAAGAAACCCCTGCTGAAGCTGAACCCAAGTTCAACCCATTTACTGGATCTGGGAGACGCTTGGACGGAAAGCCTTTGAATTATCAGCCTCCACCAGTTTCTTCTTCAGGGTCCAAGGACAAGAATCCCAGTGTTCCAAATGTTAATTCACAGTCTTCTACTGTTTCTAGTTCAAAAAATAATGCTCCTCAAACTCAAGGAAAGCTTGTGTTTGGGTCAAATCCAAATCGCAGTAAAGAGACTGAAAAGGCGCCAGCTGAGGCAAAACCAAAACAAGAGACCCCCAAAGAGAAAGAAGAAGATAAATTTCAGCCTTTCACCGGGAAGAAGTATTCTTTAAGGGGTTGATTTATATATTAAATTATATACACTTCTAAACTTTTGTTTAAAGTTATTTCGCCTTACTGTGCATGAATCCATGTCTATTGCAAATCTCGAATCTTTATTTGTTTATTGTATTATAAGCAAAAGACTGACCGGTAGATTCCATAAAAAATAACAAGTTGTTTACCGGGAGCTTAATTTTCCACCCATGGTTCTTAAAAACCTCATCYGTAACAGGTAGCAAGAATTCAGAGCATTAAGGTTTT
TARGET=77,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03577 Ps_contig_mira-and-tgicl-ass_franssen_30432
SEQUENCE=GTCGGGGGGGGTTTGTCGGTGGGGGTTGGGTTTCTCACTTTCTCTTCATTCATCTGTTTCAACACTCCAAAAAATATATTATTATTATTTATTTATATCCGTTTCGTCTTCCCCTCTCTCTGAATTCTCAGATCAAACCATCTTCTTCATCAGGAAGCTTGGAGACTTCACACTCTCGTGTTTCAATCCACACAATACAAAATGTTTTTCGAGGGATATGGATATCACGGGACATCGTTTGAGCAGACATATCGATGCTACCCTGCTTCTTTTATTGAAAAGCCCCAACTTGAAAGTGGTGACAAAATTATAATGCCGCCATCAGCCCTTGATCGTCTAGCATCTCTGCATATTGATTATCCAATGTTGTTTGAACTTCGTAATGATGCTGCTGAGCGAGTTTCTCATTGTGGTGTTCTGGAATTCATTGCAGAGGAAGGCATGATATACATGCCATACTGGATGATGGAGAACATGCTTTTACAAGAGGGGGACATTGTAAGAGTGAAAAATGCGACACTTCCAAAGGGAACATATGTTAAGTTACAGCCTCACACAAAAGACTTTTTGGATATTTCCAATCCAAAGGCTATATTAGAAACTACTTTGAGGAATTTTTCCTGCTTGACTACTGGAGATAGCATTATGGTGGCTTACAATAACAAAAAGTATTATATTGATATTATAGAAAGCAAGCCTGCCAATGCTATAAGCATCATTGAGACTGATTGTGAAGTGGACTTTGCCCCTCCCTTAGATTACAAGGAACCTGAAAAGCCCGTTCCTTCACGTTCTGCTGGAAAGGCACCAGAAGCTGATAAAGAAACCCCTGCTGAAGCTGAACCCAAGTTCAACCCATTTACTGGATCTGGGAGACGCTTGGACGGAAAGCCTTTGAATTATCAGCCTCCACCAGTTTCTTCTTCAGGGTCCAAGGACAAGAATCCCAGTGTTCCAAATGTTAATTCACAGTCTTCTACTGTTTCTAGTTCAAAAAATAATGCTCCTCAAACTCAAGGAAAGCTTGTGTTTGGGTCAAATCCAAATCGCAGTAAAGAGACTGAAAAGGCGCCAGCTGAGGCAAAACCAAAACAAGAGACCCCCAAAGAGAAAGAAGAAGATAAATTTCAGCCTTTCACCGGGAAGAAGTATTCTTTAAGGGGTTGATTTATATATTAAATTATATACACTTCTAAACTTTTGTTTAAAGTTATTTCGCCTTACTGTGCATGAATCCATGTCTATTGCAAATCTCGAATCTTTATTTGTTTATTGTATTATAAGCAAAAGACTGACCGGTAGATTCCATAAAAAATAACAAGTTGTTTACCGGGAGCTTAATTTTCCACCCATGGTTCTTAAAAACCTCATCYGTAACAGGTAGCAAGAATTCAGAGCATTAAGGTTTT
TARGET=85,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03578 Ps_contig_mira-and-tgicl-ass_franssen_30433
SEQUENCE=ACAGNGGTATNAACGTTCAGAGTACGCGGGGGATGACAAAACACCTACACCTACTCTGTGTTATCATCTATATTCTGCTTTGAAGTTGAAAGTTTAAGCACGGCAATGGCAAACGCATCTGCTTCAGAACAACAACAACACAATCTCACTCTTTATGGATATTGGAGAAGTTCATGTTCCTTCCGTGCTCGAATCGCTCTCAACCTCAAAGGTCTCAAATATCATTACAAATCAATTAATCTCTTGAAAGGAGAACAATCTCATCCCGACTTTCTTCACCTTAACCCCGTTGGTTTTGTTCCCGTTCTCGTCGATGGCGATTCCGTCATTTTCGACTCTTTCGCCATTATTATGTATTTGGAGGATAAATATCCTCAACATCCTTTGCTTCCTACTGATATTCACAAAAGAGCAATCAATTTCCAGGCTGTTAGTATTGTTTCTTCATCAATTCAACCTCTTCATAACTTAAACCTTCTGAAGTACATCGAGATAAATGTTGGCCCGGATGAAAAACTTCCATGGGTCCAGAGTGTAATTAGAAAAGGCTTCACAGCACTTGAAAAGCTATTGAAAGAACACACTGGGAGGTATGCTACTGGAGACGAAGTTTGCATGGCAGATGTATTTTTAGCACCTCAGATATATGCTGCATCTAATAGATTCAACATTGACATGAATGAATTTCCTATTCTCTCAAGATTACATGAGACATATTATGATATCCCAGCATTTCGGGAGGCTTTGCCAGAGAATCAGCCAGACGCTGTAGGCAAGCCGAGTCAATAATTTGGGACTGAAATGTGACCTGATCTTGGGTTGAAGAAATTGCACCAGGAACAACTGATTTAGCATTTGGTTGAATTTGTAATGATATACATACTCCGAAGCAATAATGTTGCCATGAAACAATAATAAATGTATCTAGTGATTCCTAATAAAAGTGTTTATATATAAAGTCAATGTAGTATGGCTCATATCATTCACCCGTGGGTATATTGGTGATTCAATCTATGAGTTTGAAAATCTTAGTGTTTAATTTTCTCATGTGTTTAGTTTCCACCCTTAGTCCGAAAATCGAGAGGCTGCTTGATTTGATGGTTTGA
TARGET=128,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2196
PRIMER_LEFT_PENALTY=0.166289
PRIMER_RIGHT_PENALTY=0.053348
PRIMER_LEFT_SEQUENCE=AAGTTGAAAGTTTAAGCACGGC
PRIMER_RIGHT_SEQUENCE=GAAAGTCGGGATGAGATTGTTC
PRIMER_LEFT=82,22
PRIMER_RIGHT=273,22
PRIMER_LEFT_TM=59.834
PRIMER_RIGHT_TM=59.947
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.1000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=0.2408
PRIMER_LEFT_1_PENALTY=0.166289
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=AAGTTGAAAGTTTAAGCACGGC
PRIMER_RIGHT_1_SEQUENCE=GAAGAAAGTCGGGATGAGATTG
PRIMER_LEFT_1=82,22
PRIMER_RIGHT_1=276,22
PRIMER_LEFT_1_TM=59.834
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=0.2408
PRIMER_LEFT_2_PENALTY=0.166289
PRIMER_RIGHT_2_PENALTY=0.074466
PRIMER_LEFT_2_SEQUENCE=AAGTTGAAAGTTTAAGCACGGC
PRIMER_RIGHT_2_SEQUENCE=TGAAGAAAGTCGGGATGAGATT
PRIMER_LEFT_2=82,22
PRIMER_RIGHT_2=277,22
PRIMER_LEFT_2_TM=59.834
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.4329
PRIMER_LEFT_3_PENALTY=0.166289
PRIMER_RIGHT_3_PENALTY=0.266650
PRIMER_LEFT_3_SEQUENCE=AAGTTGAAAGTTTAAGCACGGC
PRIMER_RIGHT_3_SEQUENCE=ACGGGGTTAAGGTGAAGAAAGT
PRIMER_LEFT_3=82,22
PRIMER_RIGHT_3=289,22
PRIMER_LEFT_3_TM=59.834
PRIMER_RIGHT_3_TM=60.267
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=11.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.5349
PRIMER_LEFT_4_PENALTY=0.166289
PRIMER_RIGHT_4_PENALTY=0.368624
PRIMER_LEFT_4_SEQUENCE=AAGTTGAAAGTTTAAGCACGGC
PRIMER_RIGHT_4_SEQUENCE=GTTAAGGTGAAGAAAGTCGGGA
PRIMER_LEFT_4=82,22
PRIMER_RIGHT_4=284,22
PRIMER_LEFT_4_TM=59.834
PRIMER_RIGHT_4_TM=59.631
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=11.1000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_03579 Ps_contig_mira-and-tgicl-ass_franssen_30435
SEQUENCE=TTACATTTACAAAGAGTAATTCAATTTGAACTTTGCTCTGTTATATCAGTAGTGAGTAGAAATAGTAATAATTAAATGAACAAAAACACAAACAATAGACTAATTTGRGACAAAAAGAAAGAAAGAAATGTTATTCTATTCTACATTTGTTGTTACATAAAACTTTTTCCCATCCCTGAACAGTGCCCATACAAACTCAAATTAATGGGAAGAAAAAGCACTAATCTTCTGCTTATTTATAAGCTGCAGTAGTTTGAGAAGATCTGAATACCGTTGAACAAGACATAACCCTTCTGGAGATGAAAATAGGGATTAGCCCACCGTTCCAAAACATGACTACTTCAGATGATGCTCGAACAAAGATGCCATCTCAATAGCAGTCAGTGCAGCTTCAGCACCCTTATTCCCAGATTTTCCACCAGCTCGATTTATAGCCTGATCCATGTCCTCACATGTTAGGACTCCAAATATGCATGGAACACCTGAGTTTAGACCAGCTGAAAGCACTCCAGATGCTGCTGAATTAGCAACTGCGTCATAGTGAGTTGTATCTCCTCTTATCACGGCTCCTATGCATATGATGGCATGATATTTGCCTGATTTACCAAGTCTTGTTGCAACAGCACCAATTTCAAAACAACCCGGAACCCACACAACATCAATGTCTTCATCTTGAACTGAATAATTCTTGAAAGTGCCCAAAGCTCCCTCCAAGAGCGGCCTGGTGATTATTTCATTAAACCGTGCAACAACCACGGCAAAACGGAGTCCCTNAGTYCTGGTAACAGAGCCCGTCAGATGCTTCACAGCAGCTGTCTGACGAAAAAGATGATCCGACCCNTGTCTTTGACATAGTGGTACTTAAACATTACTAAAGCTCGACGAAAGAAAACGAAAAGGGTC
TARGET=114,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03580 Ps_contig_mira-and-tgicl-ass_franssen_30436
SEQUENCE=GGACCACCGGTGTGCACCTGGACACGAGAATAACCGTCCACGCGTCACCGTTCTTCTCTTCACATTCACAACTCTGTCGGTGGCACTCAAGAAAGTGGGGACCACCAAGTAAGATACATACTATACTCCGAATAGATCRGGGACACGTAAAAAGAACCCTATAAATTTCGATAACCGTTTTCTTCTCAAATCTGAATGCTTTGTACTTCAACTCGCATCCTCTTTTCAACTACAACAAAACTAGCTCTAGCTGCTTCTTCTTCTTCTTCTTTTCACTTTCACTTTTTCTCCACAAATTATCCAATCTCAGTTTCTCATCTTCCAAACAAATCTTTTCCTAAACCTAAACCAAATTTTAGAACTCTAACCCCTTTGTTCTTCACTCTAAGAATAAATCACACCATGGCTTCTCCCTCAAACCCCCAATCAATTCACGATTTCACCGTTAAGGATGCTAAGGGTAATGATGTTAATCTTGGTGACTACAAAGGAAAGGTCCTTATCATCGTCAATGTTGCCTCACAATGTGGCTTGACTAACTCCAATTACACAGAACTGAGTCAGTTGTATGAGAAATACAAACAAAAAGGTTTGGAAATCCTGGCATTCCCATGCAACCAGTTTGGGGCACAGGAACCTGGATCTGTTGAAGAGATACAAGACTTTGTCTGCACTCGCTTCAAAGCTGAGTTTCCCGTTTTTGACAAGGTTGATGTGAATGGTGCCAATGCTGCTCCAATCTACAAGTATCTAAAGTCAAGCAAAGGTGGACTCTTTGGGGACGGTATCAAATGGAACTTCTCCAAGTTCCTTGTGGATAAAAATGGCAATGTTGTAGAACGTTATGCACCCACAACATCACCTCTTAGCATTGAGGTAAGACAATTTGTATAGTGCCACCACATGATATTGCCTAGTAGACGTCA
TARGET=254,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03581 Ps_contig_mira-and-tgicl-ass_franssen_30442
SEQUENCE=CCGTAAATCTTATCAACGTCCATGTGTGAACTTAATGCAGCTCCNGCANTTGNACCAAATCCAGGTACTACTTTGAGTACTCCATCCGGAATTCCAGCCTGTTTAGCGAGATGAGCATAAAACAAAGCCGAAAGAGGTGTCTGTTCAGCAGGTTTAAGAACTATAGTACAACCAGCAGCCAACGACGGAGCAACCTTAGTAGCAAACATGGTACTAGGAAAATTCCAAGGAATTATGTGTCCGACAACGCCAATAGGTTCCATTAAAGTATACGCATGCAACTCGCGCGCGGGTTTTAAAACCTCACCGTGAATTTTATCAGCAGCACCGGCGTAGTATCGGAAAGTATTTGCTACTCCAGGTATGTCAACAGCTTTGCAAAAAGTGTACAATTTTCCAGCATCTATGGTGTCTAATGCTGCTATTTCTTCTATGTTTTGATCAATTAAATCCGCCCATTTCAACAAGAATTTTGCTCTTTCGGCACCAGGCATTCGAGGCCATGGACCATCATCGAAAGCGACACGTGCCGCTCTCACAGCAACATCAATATCTTCTTTTCTTCCCTCTGCAATTTTTGCTATCACTTCTCCACTTCTAGGATCTATTGTCTCAAATTCTTTTCCTGAAAGAGAATCAACAAATTGTCCATTGATGAAGAGTTTGGTGAACTTGATGGTGGGAATCTCAAGAGAGAAATTGGGTTTGCCATTAGAGAACAGAGAAGACATGTTTGTGTTTTCCAAAGAAAGAAAGAAGAAACTAGGGACAAAGTCAGAAGGCAACTTGTTGAGTTGGGCCTTTGATTTCAAAGAAAAT
TARGET=745,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0779
PRIMER_LEFT_PENALTY=0.029253
PRIMER_RIGHT_PENALTY=0.048692
PRIMER_LEFT_SEQUENCE=TGTCCATTGATGAAGAGTTTGG
PRIMER_RIGHT_SEQUENCE=CAACTCAACAAGTTGCCTTCTG
PRIMER_LEFT=645,22
PRIMER_RIGHT=796,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=59.951
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=0.0779
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.048692
PRIMER_LEFT_1_SEQUENCE=TCCATTGATGAAGAGTTTGGTG
PRIMER_RIGHT_1_SEQUENCE=CAACTCAACAAGTTGCCTTCTG
PRIMER_LEFT_1=647,22
PRIMER_RIGHT_1=796,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.951
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=150
PRIMER_PAIR_PENALTY_2=0.0779
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.048692
PRIMER_LEFT_2_SEQUENCE=CCATTGATGAAGAGTTTGGTGA
PRIMER_RIGHT_2_SEQUENCE=CAACTCAACAAGTTGCCTTCTG
PRIMER_LEFT_2=648,22
PRIMER_RIGHT_2=796,22
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=59.951
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.2052
PRIMER_LEFT_3_PENALTY=0.156479
PRIMER_RIGHT_3_PENALTY=0.048692
PRIMER_LEFT_3_SEQUENCE=GAAATTGGGTTTGCCATTAGAG
PRIMER_RIGHT_3_SEQUENCE=CAACTCAACAAGTTGCCTTCTG
PRIMER_LEFT_3=695,22
PRIMER_RIGHT_3=796,22
PRIMER_LEFT_3_TM=59.844
PRIMER_RIGHT_3_TM=59.951
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=102
PRIMER_PAIR_PENALTY_4=0.2052
PRIMER_LEFT_4_PENALTY=0.156479
PRIMER_RIGHT_4_PENALTY=0.048692
PRIMER_LEFT_4_SEQUENCE=GAGAAATTGGGTTTGCCATTAG
PRIMER_RIGHT_4_SEQUENCE=CAACTCAACAAGTTGCCTTCTG
PRIMER_LEFT_4=693,22
PRIMER_RIGHT_4=796,22
PRIMER_LEFT_4_TM=59.844
PRIMER_RIGHT_4_TM=59.951
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=104
=
PRIMER_SEQUENCE_ID=SSR_03582 Ps_contig_mira-and-tgicl-ass_franssen_30451
SEQUENCE=ATAGTAGGAAGATTGAGAGAAGAGATTCTAACTAGAGCCAGAACATGCGCGCTAGTGTTGAAGCGTGAAAGGTGCGAACGAACGCCATGTCTGGCATTACCACCACCACCGTTCCCGTTAAACTCAAACTCTTCGATACCAGAATCTCCCCACTCTTCTCTTCTTCTATTCCTCATTCCCTCAGTTTCCACTATTCCCCCTCCCACGCAACCGTCCGTTACGGTGTTAGCCTCCGCACTTCCGCATACGGCAACAACTTCAGAAGACCACCGCCTAGCAACGAAGCCGACAATGAAGAAGCTCTTGACCTCTCCAAAATTAGGTCGGGTACCGTTAGGCTTATTGATCAGAGTCAGAATATGATTGGAGTTGTGTCTCTTGATAAGGCAATTCAAATGGCTGAAGATGCGGAACTCGACTTGGTGATAGTATCTGCGGAGGCAGATCCTCCTGTTGTTAGAATAATGAATTACAGTAAATATAAATATGAAATGCAGAAGAAGAAAAGAGATCAGCAGAAAAAAAGTGCTGCTAGTAGGATGGATTTGAAGGAGCTCAAAATGGGATACAACATAGATCAACATGATTATTCTGTACGTTTGAAAGCTGCACGGAAATTTTTGGCCGATGGTGACAAGGTTAAGATCATAGTAAACCTTAAAGGCCGTGAAAAAGAATTCAGGAATAATGCTATTGAGCTTATTAGACGTTTTCAGGACGATGTTGGGAAGCTTGGAACAGAGGAGGCTAAAAACTTCCGTGACAAGAACATCTTCATAACTCTTGTTCCAAATAAAGTTGAAGTGCAGAAACCTCAAGAAACACCGAAAATAGCTGCAGCAGATGAAGTCTCTGTCAGTGTCGAAGCATAGTTGAAAAAAATTTCCACACGGGATTCTAATTGTAGAAGGGATGGATTGTGAGTTACTTATATTGCTCTGTTTTGAAATTTGAGTGTTGTACGTATAGAATGCATATAATTCATGTGATAATAGTATATAGTTCATTAGAAGTTGAATTATAGAGGCAGCCTTGTCCATTAACCGATCTCTTCGGTAGTAATCACACAAGATCACCCGGGATGAAAATCTTCTGCAGTTACTTGATTTGCAATGTATCTCAACCTTTGAATTTCATTTTTTTAATCCCTCTCTGGGAAAAAAATGCCAGCCTGGGTGATATAGTTGATTATGACTCAGTACCTGTTTGAGTGCAGCTTTATTTTATTAACCTTGACTACAAANATAGTTAATTTTCTTGTTTAAGAATAAAAAGT
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5321
PRIMER_LEFT_PENALTY=0.338748
PRIMER_RIGHT_PENALTY=0.193337
PRIMER_LEFT_SEQUENCE=TAGTGTTGAAGCGTGAAAGGTG
PRIMER_RIGHT_SEQUENCE=CTTCTGAAGTTGTTGCCGTATG
PRIMER_LEFT=52,22
PRIMER_RIGHT=264,22
PRIMER_LEFT_TM=60.339
PRIMER_RIGHT_TM=59.807
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.7627
PRIMER_LEFT_1_PENALTY=0.338748
PRIMER_RIGHT_1_PENALTY=0.423908
PRIMER_LEFT_1_SEQUENCE=TAGTGTTGAAGCGTGAAAGGTG
PRIMER_RIGHT_1_SEQUENCE=GAAGAGTGGGGAGATTCTGGTA
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=157,22
PRIMER_LEFT_1_TM=60.339
PRIMER_RIGHT_1_TM=59.576
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=0.8132
PRIMER_LEFT_2_PENALTY=0.338748
PRIMER_RIGHT_2_PENALTY=0.474473
PRIMER_LEFT_2_SEQUENCE=TAGTGTTGAAGCGTGAAAGGTG
PRIMER_RIGHT_2_SEQUENCE=CGACCTAATTTTGGAGAGGTCA
PRIMER_LEFT_2=52,22
PRIMER_RIGHT_2=325,22
PRIMER_LEFT_2_TM=60.339
PRIMER_RIGHT_2_TM=60.474
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=0.8295
PRIMER_LEFT_3_PENALTY=0.338748
PRIMER_RIGHT_3_PENALTY=0.490741
PRIMER_LEFT_3_SEQUENCE=TAGTGTTGAAGCGTGAAAGGTG
PRIMER_RIGHT_3_SEQUENCE=AGAAGAGTGGGGAGATTCTGGT
PRIMER_LEFT_3=52,22
PRIMER_RIGHT_3=158,22
PRIMER_LEFT_3_TM=60.339
PRIMER_RIGHT_3_TM=60.491
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=0.8417
PRIMER_LEFT_4_PENALTY=0.338748
PRIMER_RIGHT_4_PENALTY=0.502921
PRIMER_LEFT_4_SEQUENCE=TAGTGTTGAAGCGTGAAAGGTG
PRIMER_RIGHT_4_SEQUENCE=TACCCGACCTAATTTTGGAGAG
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=329,22
PRIMER_LEFT_4_TM=60.339
PRIMER_RIGHT_4_TM=59.497
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_03583 Ps_contig_mira-and-tgicl-ass_franssen_30457
SEQUENCE=TTCTCTTGCATATTCCTTTCGTCCATCTTCCTCCCTTTTGTCTTGTGTATGTATGTATGTATGCACCTTGAATTTCACATATACATACATACATACACACCTATCTTCTAACCGTTTTTGTTTGATTTCTCTCTCTCTCTCTCTCTGCATTTTGCTCAATCCCTAACTAATTCCGATTCAATTCAACTTTTGATTATCATGGACGGTTTAGTCAACAGCGTTAACGGTGTAGTTGAAAACGTTGAGAAGCTCATTATTGATACTGACCCTGGGATCGATGATAGTATGGCGATTATGATGGCATTTAGTTGTCCTCAGGTGGAGGTGGTGGGTTTGACAACGGTTTTTGGTAATGCTGCTACTGAAGATGCCACACGTAATGCGTTGCTTTTGTGTGAGATTGCAGGCCGTCAAAATCTTCCTGTTGCAGAGGGAAGCATTGAGCCATTGAAGGGTGGAAGGCCTCGTGTTGCTGATTTTGTTCATGGTAAAGATGGATTAGGGAACATATTCTTACCTGATCCTAAAGCTAATAAAATTGATAAAAGTGCTAGTGAGTTTTTAGTGGAGAAGGTATCTGAATGTCCTGGTGAAGTAACTGTGCTAGCACTTGGACCACTTACAAACATAGCTCTAGCAATCAAAAGAGATTCTTCTTTTGCAAGCAATGTGAAGAGAATAGTTGTGCTTGGTGGTGCATTTTTCGCCTTGGGGAATGTCAATCCAGCTGCAGAAGCAAATATATATGGAGACCCAGAAGCAGCAGATATTGTGTTTACCTCTGGGGCAGATGTTGTTGTTGTTGGCATTAACATCACAACCCAAGTGCAACTTACAGATGCTGATCTCCTTGAACTGAAGGAATCAAAAGGAAAGCATGCATCTCTTTTAAGTGACATGTGCAAATTCTATAGGGATTGGCATGTAAAATCTGACGGTGTCTATGGAATTTTCCTACACGACCCTGTTAGTTTTGTGGCAGTTTTGCGTCCAGATCTTTTCACATACAAGAAGGGAGTAGTGAGGGTAGAGACACAAGGCCTCTGTGTTGGACATACACTTCTAGACCAAGGACTAAAAAGATGGAATACGAGCAATCCATGGACGGGTTATTCACCGATTTCAGTAGCTTGGACTGTGAATGTTGATGGGGTTCTTAGTTACATTAAGGAGTTGTTGATGAAACCCTGAAGAATAGGCATTTGTACATGTCAAAGCTTTGGAAAAAGTTATGTATAAGGTCATTCAATTTATTCCACTAATTGTCAACTAGAACTTTGGGTATCAAGGGCACATAACAGTTCCCCTGATCTTCTGGCCTACATGTCCTTGTCCCTTTCAATCTTAGAAATTTATGTACCAATAAAATTCTCTTAATAAGATATAATTTCTTTTCGATTCCACTGGAATGACAAGTACTATTGCAGGGTCATCCTGAAAACC
TARGET=46,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1257
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=CTCTTGCATATTCCTTTCGTCC
PRIMER_RIGHT_SEQUENCE=CTGTTGACTAAACCGTCCATGA
PRIMER_LEFT=2,22
PRIMER_RIGHT=217,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=0.1328
PRIMER_LEFT_1_PENALTY=0.102490
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=CATCTTCCTCCCTTTTGTCTTG
PRIMER_RIGHT_1_SEQUENCE=CTGTTGACTAAACCGTCCATGA
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=217,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=0.2600
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.164560
PRIMER_LEFT_2_SEQUENCE=CTCTTGCATATTCCTTTCGTCC
PRIMER_RIGHT_2_SEQUENCE=ATCGCCATACTATCATCGATCC
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=292,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.165
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.2671
PRIMER_LEFT_3_PENALTY=0.102490
PRIMER_RIGHT_3_PENALTY=0.164560
PRIMER_LEFT_3_SEQUENCE=CATCTTCCTCCCTTTTGTCTTG
PRIMER_RIGHT_3_SEQUENCE=ATCGCCATACTATCATCGATCC
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=292,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.165
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.3622
PRIMER_LEFT_4_PENALTY=0.331942
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=CTTGCATATTCCTTTCGTCCAT
PRIMER_RIGHT_4_SEQUENCE=CTGTTGACTAAACCGTCCATGA
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=60.332
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_03584 Ps_contig_mira-and-tgicl-ass_franssen_30457
SEQUENCE=TTCTCTTGCATATTCCTTTCGTCCATCTTCCTCCCTTTTGTCTTGTGTATGTATGTATGTATGCACCTTGAATTTCACATATACATACATACATACACACCTATCTTCTAACCGTTTTTGTTTGATTTCTCTCTCTCTCTCTCTCTGCATTTTGCTCAATCCCTAACTAATTCCGATTCAATTCAACTTTTGATTATCATGGACGGTTTAGTCAACAGCGTTAACGGTGTAGTTGAAAACGTTGAGAAGCTCATTATTGATACTGACCCTGGGATCGATGATAGTATGGCGATTATGATGGCATTTAGTTGTCCTCAGGTGGAGGTGGTGGGTTTGACAACGGTTTTTGGTAATGCTGCTACTGAAGATGCCACACGTAATGCGTTGCTTTTGTGTGAGATTGCAGGCCGTCAAAATCTTCCTGTTGCAGAGGGAAGCATTGAGCCATTGAAGGGTGGAAGGCCTCGTGTTGCTGATTTTGTTCATGGTAAAGATGGATTAGGGAACATATTCTTACCTGATCCTAAAGCTAATAAAATTGATAAAAGTGCTAGTGAGTTTTTAGTGGAGAAGGTATCTGAATGTCCTGGTGAAGTAACTGTGCTAGCACTTGGACCACTTACAAACATAGCTCTAGCAATCAAAAGAGATTCTTCTTTTGCAAGCAATGTGAAGAGAATAGTTGTGCTTGGTGGTGCATTTTTCGCCTTGGGGAATGTCAATCCAGCTGCAGAAGCAAATATATATGGAGACCCAGAAGCAGCAGATATTGTGTTTACCTCTGGGGCAGATGTTGTTGTTGTTGGCATTAACATCACAACCCAAGTGCAACTTACAGATGCTGATCTCCTTGAACTGAAGGAATCAAAAGGAAAGCATGCATCTCTTTTAAGTGACATGTGCAAATTCTATAGGGATTGGCATGTAAAATCTGACGGTGTCTATGGAATTTTCCTACACGACCCTGTTAGTTTTGTGGCAGTTTTGCGTCCAGATCTTTTCACATACAAGAAGGGAGTAGTGAGGGTAGAGACACAAGGCCTCTGTGTTGGACATACACTTCTAGACCAAGGACTAAAAAGATGGAATACGAGCAATCCATGGACGGGTTATTCACCGATTTCAGTAGCTTGGACTGTGAATGTTGATGGGGTTCTTAGTTACATTAAGGAGTTGTTGATGAAACCCTGAAGAATAGGCATTTGTACATGTCAAAGCTTTGGAAAAAGTTATGTATAAGGTCATTCAATTTATTCCACTAATTGTCAACTAGAACTTTGGGTATCAAGGGCACATAACAGTTCCCCTGATCTTCTGGCCTACATGTCCTTGTCCCTTTCAATCTTAGAAATTTATGTACCAATAAAATTCTCTTAATAAGATATAATTTCTTTTCGATTCCACTGGAATGACAAGTACTATTGCAGGGTCATCCTGAAAACC
TARGET=81,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1257
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=CTCTTGCATATTCCTTTCGTCC
PRIMER_RIGHT_SEQUENCE=CTGTTGACTAAACCGTCCATGA
PRIMER_LEFT=2,22
PRIMER_RIGHT=217,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=0.1328
PRIMER_LEFT_1_PENALTY=0.102490
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=CATCTTCCTCCCTTTTGTCTTG
PRIMER_RIGHT_1_SEQUENCE=CTGTTGACTAAACCGTCCATGA
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=217,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=0.2600
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.164560
PRIMER_LEFT_2_SEQUENCE=CTCTTGCATATTCCTTTCGTCC
PRIMER_RIGHT_2_SEQUENCE=ATCGCCATACTATCATCGATCC
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=292,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.165
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.2671
PRIMER_LEFT_3_PENALTY=0.102490
PRIMER_RIGHT_3_PENALTY=0.164560
PRIMER_LEFT_3_SEQUENCE=CATCTTCCTCCCTTTTGTCTTG
PRIMER_RIGHT_3_SEQUENCE=ATCGCCATACTATCATCGATCC
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=292,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.165
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.3622
PRIMER_LEFT_4_PENALTY=0.331942
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=CTTGCATATTCCTTTCGTCCAT
PRIMER_RIGHT_4_SEQUENCE=CTGTTGACTAAACCGTCCATGA
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=60.332
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_03585 Ps_contig_mira-and-tgicl-ass_franssen_30457
SEQUENCE=TTCTCTTGCATATTCCTTTCGTCCATCTTCCTCCCTTTTGTCTTGTGTATGTATGTATGTATGCACCTTGAATTTCACATATACATACATACATACACACCTATCTTCTAACCGTTTTTGTTTGATTTCTCTCTCTCTCTCTCTCTGCATTTTGCTCAATCCCTAACTAATTCCGATTCAATTCAACTTTTGATTATCATGGACGGTTTAGTCAACAGCGTTAACGGTGTAGTTGAAAACGTTGAGAAGCTCATTATTGATACTGACCCTGGGATCGATGATAGTATGGCGATTATGATGGCATTTAGTTGTCCTCAGGTGGAGGTGGTGGGTTTGACAACGGTTTTTGGTAATGCTGCTACTGAAGATGCCACACGTAATGCGTTGCTTTTGTGTGAGATTGCAGGCCGTCAAAATCTTCCTGTTGCAGAGGGAAGCATTGAGCCATTGAAGGGTGGAAGGCCTCGTGTTGCTGATTTTGTTCATGGTAAAGATGGATTAGGGAACATATTCTTACCTGATCCTAAAGCTAATAAAATTGATAAAAGTGCTAGTGAGTTTTTAGTGGAGAAGGTATCTGAATGTCCTGGTGAAGTAACTGTGCTAGCACTTGGACCACTTACAAACATAGCTCTAGCAATCAAAAGAGATTCTTCTTTTGCAAGCAATGTGAAGAGAATAGTTGTGCTTGGTGGTGCATTTTTCGCCTTGGGGAATGTCAATCCAGCTGCAGAAGCAAATATATATGGAGACCCAGAAGCAGCAGATATTGTGTTTACCTCTGGGGCAGATGTTGTTGTTGTTGGCATTAACATCACAACCCAAGTGCAACTTACAGATGCTGATCTCCTTGAACTGAAGGAATCAAAAGGAAAGCATGCATCTCTTTTAAGTGACATGTGCAAATTCTATAGGGATTGGCATGTAAAATCTGACGGTGTCTATGGAATTTTCCTACACGACCCTGTTAGTTTTGTGGCAGTTTTGCGTCCAGATCTTTTCACATACAAGAAGGGAGTAGTGAGGGTAGAGACACAAGGCCTCTGTGTTGGACATACACTTCTAGACCAAGGACTAAAAAGATGGAATACGAGCAATCCATGGACGGGTTATTCACCGATTTCAGTAGCTTGGACTGTGAATGTTGATGGGGTTCTTAGTTACATTAAGGAGTTGTTGATGAAACCCTGAAGAATAGGCATTTGTACATGTCAAAGCTTTGGAAAAAGTTATGTATAAGGTCATTCAATTTATTCCACTAATTGTCAACTAGAACTTTGGGTATCAAGGGCACATAACAGTTCCCCTGATCTTCTGGCCTACATGTCCTTGTCCCTTTCAATCTTAGAAATTTATGTACCAATAAAATTCTCTTAATAAGATATAATTTCTTTTCGATTCCACTGGAATGACAAGTACTATTGCAGGGTCATCCTGAAAACC
TARGET=128,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1257
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=CTCTTGCATATTCCTTTCGTCC
PRIMER_RIGHT_SEQUENCE=CTGTTGACTAAACCGTCCATGA
PRIMER_LEFT=2,22
PRIMER_RIGHT=217,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=0.1328
PRIMER_LEFT_1_PENALTY=0.102490
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=CATCTTCCTCCCTTTTGTCTTG
PRIMER_RIGHT_1_SEQUENCE=CTGTTGACTAAACCGTCCATGA
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=217,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=195
PRIMER_PAIR_PENALTY_2=0.2600
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.164560
PRIMER_LEFT_2_SEQUENCE=CTCTTGCATATTCCTTTCGTCC
PRIMER_RIGHT_2_SEQUENCE=ATCGCCATACTATCATCGATCC
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=292,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.165
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.2671
PRIMER_LEFT_3_PENALTY=0.102490
PRIMER_RIGHT_3_PENALTY=0.164560
PRIMER_LEFT_3_SEQUENCE=CATCTTCCTCCCTTTTGTCTTG
PRIMER_RIGHT_3_SEQUENCE=ATCGCCATACTATCATCGATCC
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=292,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.165
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.3622
PRIMER_LEFT_4_PENALTY=0.331942
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=CTTGCATATTCCTTTCGTCCAT
PRIMER_RIGHT_4_SEQUENCE=CTGTTGACTAAACCGTCCATGA
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=60.332
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_03586 Ps_contig_mira-and-tgicl-ass_franssen_30457
SEQUENCE=TTCTCTTGCATATTCCTTTCGTCCATCTTCCTCCCTTTTGTCTTGTGTATGTATGTATGTATGCACCTTGAATTTCACATATACATACATACATACACACCTATCTTCTAACCGTTTTTGTTTGATTTCTCTCTCTCTCTCTCTCTGCATTTTGCTCAATCCCTAACTAATTCCGATTCAATTCAACTTTTGATTATCATGGACGGTTTAGTCAACAGCGTTAACGGTGTAGTTGAAAACGTTGAGAAGCTCATTATTGATACTGACCCTGGGATCGATGATAGTATGGCGATTATGATGGCATTTAGTTGTCCTCAGGTGGAGGTGGTGGGTTTGACAACGGTTTTTGGTAATGCTGCTACTGAAGATGCCACACGTAATGCGTTGCTTTTGTGTGAGATTGCAGGCCGTCAAAATCTTCCTGTTGCAGAGGGAAGCATTGAGCCATTGAAGGGTGGAAGGCCTCGTGTTGCTGATTTTGTTCATGGTAAAGATGGATTAGGGAACATATTCTTACCTGATCCTAAAGCTAATAAAATTGATAAAAGTGCTAGTGAGTTTTTAGTGGAGAAGGTATCTGAATGTCCTGGTGAAGTAACTGTGCTAGCACTTGGACCACTTACAAACATAGCTCTAGCAATCAAAAGAGATTCTTCTTTTGCAAGCAATGTGAAGAGAATAGTTGTGCTTGGTGGTGCATTTTTCGCCTTGGGGAATGTCAATCCAGCTGCAGAAGCAAATATATATGGAGACCCAGAAGCAGCAGATATTGTGTTTACCTCTGGGGCAGATGTTGTTGTTGTTGGCATTAACATCACAACCCAAGTGCAACTTACAGATGCTGATCTCCTTGAACTGAAGGAATCAAAAGGAAAGCATGCATCTCTTTTAAGTGACATGTGCAAATTCTATAGGGATTGGCATGTAAAATCTGACGGTGTCTATGGAATTTTCCTACACGACCCTGTTAGTTTTGTGGCAGTTTTGCGTCCAGATCTTTTCACATACAAGAAGGGAGTAGTGAGGGTAGAGACACAAGGCCTCTGTGTTGGACATACACTTCTAGACCAAGGACTAAAAAGATGGAATACGAGCAATCCATGGACGGGTTATTCACCGATTTCAGTAGCTTGGACTGTGAATGTTGATGGGGTTCTTAGTTACATTAAGGAGTTGTTGATGAAACCCTGAAGAATAGGCATTTGTACATGTCAAAGCTTTGGAAAAAGTTATGTATAAGGTCATTCAATTTATTCCACTAATTGTCAACTAGAACTTTGGGTATCAAGGGCACATAACAGTTCCCCTGATCTTCTGGCCTACATGTCCTTGTCCCTTTCAATCTTAGAAATTTATGTACCAATAAAATTCTCTTAATAAGATATAATTTCTTTTCGATTCCACTGGAATGACAAGTACTATTGCAGGGTCATCCTGAAAACC
TARGET=792,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1275
PRIMER_LEFT_PENALTY=0.053515
PRIMER_RIGHT_PENALTY=0.073989
PRIMER_LEFT_SEQUENCE=CTTTTGCAAGCAATGTGAAGAG
PRIMER_RIGHT_SEQUENCE=GTAAGTTGCACTTGGGTTGTGA
PRIMER_LEFT=655,22
PRIMER_RIGHT=835,22
PRIMER_LEFT_TM=60.054
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.1480
PRIMER_LEFT_1_PENALTY=0.073989
PRIMER_RIGHT_1_PENALTY=0.073989
PRIMER_LEFT_1_SEQUENCE=GCACTTGGACCACTTACAAACA
PRIMER_RIGHT_1_SEQUENCE=GTAAGTTGCACTTGGGTTGTGA
PRIMER_LEFT_1=606,22
PRIMER_RIGHT_1=835,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.1941
PRIMER_LEFT_2_PENALTY=0.158297
PRIMER_RIGHT_2_PENALTY=0.035772
PRIMER_LEFT_2_SEQUENCE=ACCCAGAAGCAGCAGATATTGT
PRIMER_RIGHT_2_SEQUENCE=ACTGCCACAAAACTAACAGGGT
PRIMER_LEFT_2=751,22
PRIMER_RIGHT_2=982,22
PRIMER_LEFT_2_TM=60.158
PRIMER_RIGHT_2_TM=59.964
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.2569
PRIMER_LEFT_3_PENALTY=0.053515
PRIMER_RIGHT_3_PENALTY=0.203343
PRIMER_LEFT_3_SEQUENCE=CTTTTGCAAGCAATGTGAAGAG
PRIMER_RIGHT_3_SEQUENCE=TCTGTAAGTTGCACTTGGGTTG
PRIMER_LEFT_3=655,22
PRIMER_RIGHT_3=838,22
PRIMER_LEFT_3_TM=60.054
PRIMER_RIGHT_3_TM=60.203
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.2575
PRIMER_LEFT_4_PENALTY=0.053515
PRIMER_RIGHT_4_PENALTY=0.203971
PRIMER_LEFT_4_SEQUENCE=CTTTTGCAAGCAATGTGAAGAG
PRIMER_RIGHT_4_SEQUENCE=CAGATTTTACATGCCAATCCCT
PRIMER_LEFT_4=655,22
PRIMER_RIGHT_4=933,22
PRIMER_LEFT_4_TM=60.054
PRIMER_RIGHT_4_TM=60.204
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=279
=
PRIMER_SEQUENCE_ID=SSR_03587 Ps_contig_mira-and-tgicl-ass_franssen_30464
SEQUENCE=GCTCTATCAATTTTTCTATCTTCATTATCATAAGATTCACAACACAGGTAGGGGTAAAACCTATAAGCCCAACTTTTGTAAAATACATATTTGATGTAATCAATCACTTATAATTTAATTAAAAAACAATGCTGTTAGAGTTTGAGACATGAACGAGTAGCGAGATTCAAGAAACATAAAGCTTTGTAGCAGCCTGGCTGGAATAACAATCATTCAAAATCACACACAGGGCAGCTAGGGAGGATATATCCCAGTGAACGTAGAAGCACAAAACCAAGGCGGTGGTACCAGAAGACACCTRCNTAYAGAGAAAAACATATATTTTAGCAAGATCCGGAACAATTTTTAACAAACTACTAGAAAGCAACTACTCTTAAAAAACTGTTTTACCTGCAACAAAACTTGAAGAATTCGGTGTAGATAACATAACACAAGTCAGTAAATCAAGAAAGTAACTAAACCATTTAGGGTAGAAGCTCAGGGCACTGCCCAACAAAACAGATGTGTACTGCAGTTGAAAACACTAGATTAACAATATCTCGGTATCTTCTGAACAGTCGCAGTTACCGACTTTTAAAAATCAACTTCTGCTTCGAGGTGCATCCCTTAACCCATTTCCGTTAGCATGTGCTACGGCCTCACGTCCACGGTAAGGAGGGGGTGACCTGCTGTAGCTTCGGCGACGAGGTGAAGGGCTGCGACGACGAGGAGATGGGCTTCGCCGCCTAGGGGACCGCTCACGAGGACTGTAACTCCTTCGCCCATAACTCGGGCTCCTACGCAATCGAGGTGGACTGCGGCTCCGACGCCTTCCACCACCACCACCACGAGAAGAATGACACTCCCGGGCAAAATGACCAGGTTCACCACACTCATAACATTTCAAATCAGAACCACCCCCACCTGACCGACCACGACCACCACCGCCACCACCTCTTCCTCCTCCTCCACCTCCAGTTTTAGAGTTGTGAGAAAGCTCTACCCTCCAACCATTTTTACCATCTAATTCGCGGATAGCATCCAGCGCGTCTCTGCGGTCATCAAAATCAATGAAAGCATAACCAGGAGGCCTCCTTGCAACCCACACACTTCGTATAACTCCAAAAACATGAAAATCATCTTCCAGATCCCTCTCAGAAACTCGTGAATCCAGATTTCCAATATACACGCGAGTCATTGTTTCCGCTGAAGGCGATGTGGTGAATATTGAAAAAGGGGGGATAAACCCTAGCAGCAGCCNCNGACAGTCTTTTGTTTGTCACCG
TARGET=813,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03588 Ps_contig_mira-and-tgicl-ass_franssen_30464
SEQUENCE=GCTCTATCAATTTTTCTATCTTCATTATCATAAGATTCACAACACAGGTAGGGGTAAAACCTATAAGCCCAACTTTTGTAAAATACATATTTGATGTAATCAATCACTTATAATTTAATTAAAAAACAATGCTGTTAGAGTTTGAGACATGAACGAGTAGCGAGATTCAAGAAACATAAAGCTTTGTAGCAGCCTGGCTGGAATAACAATCATTCAAAATCACACACAGGGCAGCTAGGGAGGATATATCCCAGTGAACGTAGAAGCACAAAACCAAGGCGGTGGTACCAGAAGACACCTRCNTAYAGAGAAAAACATATATTTTAGCAAGATCCGGAACAATTTTTAACAAACTACTAGAAAGCAACTACTCTTAAAAAACTGTTTTACCTGCAACAAAACTTGAAGAATTCGGTGTAGATAACATAACACAAGTCAGTAAATCAAGAAAGTAACTAAACCATTTAGGGTAGAAGCTCAGGGCACTGCCCAACAAAACAGATGTGTACTGCAGTTGAAAACACTAGATTAACAATATCTCGGTATCTTCTGAACAGTCGCAGTTACCGACTTTTAAAAATCAACTTCTGCTTCGAGGTGCATCCCTTAACCCATTTCCGTTAGCATGTGCTACGGCCTCACGTCCACGGTAAGGAGGGGGTGACCTGCTGTAGCTTCGGCGACGAGGTGAAGGGCTGCGACGACGAGGAGATGGGCTTCGCCGCCTAGGGGACCGCTCACGAGGACTGTAACTCCTTCGCCCATAACTCGGGCTCCTACGCAATCGAGGTGGACTGCGGCTCCGACGCCTTCCACCACCACCACCACGAGAAGAATGACACTCCCGGGCAAAATGACCAGGTTCACCACACTCATAACATTTCAAATCAGAACCACCCCCACCTGACCGACCACGACCACCACCGCCACCACCTCTTCCTCCTCCTCCACCTCCAGTTTTAGAGTTGTGAGAAAGCTCTACCCTCCAACCATTTTTACCATCTAATTCGCGGATAGCATCCAGCGCGTCTCTGCGGTCATCAAAATCAATGAAAGCATAACCAGGAGGCCTCCTTGCAACCCACACACTTCGTATAACTCCAAAAACATGAAAATCATCTTCCAGATCCCTCTCAGAAACTCGTGAATCCAGATTTCCAATATACACGCGAGTCATTGTTTCCGCTGAAGGCGATGTGGTGAATATTGAAAAAGGGGGGATAAACCCTAGCAGCAGCCNCNGACAGTCTTTTGTTTGTCACCG
TARGET=938,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03589 Ps_contig_mira-and-tgicl-ass_franssen_30467
SEQUENCE=TACGTCGGGGGTTAGAAGACTTTNGAAGAGTTTCCACTTTCAACAACATCTGGAGACAGACAATATAGCAGTTAGATAACACATAGCCGCCTCATAATTCATCTTCTTCTCTCTTCTTCTTCTCATGAATCAAAACTAGAAGAAGAAACAGAAACAAACAAAAAATGGGTTGTGGAAACATGTTCTTCACAATTCTCATAACATTTTCAGTTACACTCATAACATACAACATAATCATCTCAGGAAACGCTCCTTTGAAGCAAGATTTTCCAGGTGGTCCTTCACGTCGTCCTTCAATTACCATTGACCCAATAATCAAGATGCCATTGGAGAAGCAAAAATCATCATCGAGGAGATTGTTTCATACTGCTGTTACAGCTTCTGATTCTGTTTACAATACTTGGCAGTGTAGGGTTATGTATTATTGGTTCAAAAAGTTTAAGGAAGATTCAGATTCTGATATGGGTGGATTTACCAGGATTTTGCATTCTGGTAAAGCTGATCAGTTTATGGATGAGATCCCTACTTTTGTTGCTCAGCCTTTGCCTTCTGGAATGGATCAGGGTTACATAGTTCTTAATAGACCTTGGGCATTTGTACAGTGGCTTCAACAAGCAGATATCAAAGAAGATTACATATTGATGTCGGAGCCGGATCATATAATTATGAAACCTATACCGAATTTAGCCAGAAATGGAATGGGAGCTGCGTTCCCTTTCTTTTATATTGAGCCTAAGAAGTACGAGAATGTGCTAAGGAAGTACTTCCCGGAGGAAAATGGACCTGTGACGAATATAGATCCAATTGGGAATTCGCCGGTGATTGTTGGCAAGGAATCGCTTAAGAAGATTGCTCCTACTTGGATGAATGTTTCGTTGGCGATGAAAAAAGATCCTGAAACTGATAAAGCTTTCGGATGGGTGCTTGAAATGTATGCTTATGCTGTTGCATCTGCTCTTCATGGTGTTCGTAACATATTACACAAGGACTTTATGATTCAGCCTCCATGGGACAAAGAACTTGGCAACAAGTACATAATTCACTACACTTATGGCTGTGACTATAATATGAAGGGCGAACTGACATATGGAAAGATTGGAGAATGGAGGTTTGATAAAAGATCTTATGATGGTGTTGCTCCTCCAAAGAATCTGACTTTGCCACCACCTGGTGTTCCAGAAAGCGTGGTGACCCTTGTAAAAATGGTTAATGAAGCTACAGCAAATATTCCGAACTGGCCATCATAGGAATTTCTCATGGTGGTTGCAATGTGTTCAGAATGCTGGAGCTCTTTGCTATTTCACACATGTTGGCAACTGTGTATCTGTAACTCTTTATGTAGATGACTTTACACACTTGAGGCAAACATGGATATTTTTTCAATGCTTGATTAAAAAAAATGTGGTGTGTTTTCAGAATGTGTCCAAATTGGAAAGTTTTCTTTTAATTTTATTGATTGCTGTAGTTGAGAAGATTGACAATGAATCAAGAAGGAACAAAACAATTGTGCTCAAATCTGTAACCCTGTATCTTTGTAGTATACTAATAAAACTTCATTTCAGTRNTGAACCAAAAA
TARGET=112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03590 Ps_contig_mira-and-tgicl-ass_franssen_30472
SEQUENCE=TTCTCTTCTTGTTGTTGCTGCTACTGTTCCCTTTTCTCTTCTCAACTTCCCTCTCCAGAACCATTCTAATAAGATCCAATTGCGTGTCACATTGCTTCATTGTTTTCTTCCTAACAGAAACAGTAGCATGAGCAGATGCAGATGCAGATGCAGAAGCAGAAGGAGTGTCGGTTAACGATTCTGAATCCTCCGAGAACTTCCGCTTCTTCTTCTTGATCTTTCTCAGCTCCATTTTGAACTCACTCTGTGCCCTAGCTGTTTCCGTTTCCGCATTGTCGATATCAACAGTTTCATCCACGTTTTTCCGCTTCCTCTGAAGCTTCAGAGACTCAGGAACTTTCTCTTTGTATTGACGATGCTTTCCCTGGAGGTAGTGAATCTCGCAGAGCTTCAGATCATCCATCACTCTCCGCTTGCACCGCCATTGCTTCCCGTCGTTTCATCCGCACCGGAGATGGTCCGGTGGAACCTCCTCGTTCGCCGATGCTGATCCTTTCTTCATTATAATCTAGTTTTTTTGGGGAAGAATTGGGATTTTTTGTAGAGGGAATTGGATGGGGAAAGTTAAACCTAATTTCCCAGTGACAGAAATTATATTTTTCTGCATAGAGCACAGGGTAAGGGTTTTTATTTGAAATTGTTTGTTTTTTTTAATTCAATTTATAAATGAACTGAATTATGCAAGGTT
TARGET=203,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0937
PRIMER_LEFT_PENALTY=0.012411
PRIMER_RIGHT_PENALTY=0.081328
PRIMER_LEFT_SEQUENCE=CTCTTCTCAACTTCCCTCTCCA
PRIMER_RIGHT_SEQUENCE=AGCTAGGGCACAGAGTGAGTTC
PRIMER_LEFT=35,22
PRIMER_RIGHT=256,22
PRIMER_LEFT_TM=59.988
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.0937
PRIMER_LEFT_1_PENALTY=0.012411
PRIMER_RIGHT_1_PENALTY=0.081328
PRIMER_LEFT_1_SEQUENCE=TCTTCTCAACTTCCCTCTCCAG
PRIMER_RIGHT_1_SEQUENCE=AGCTAGGGCACAGAGTGAGTTC
PRIMER_LEFT_1=36,22
PRIMER_RIGHT_1=256,22
PRIMER_LEFT_1_TM=59.988
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.0988
PRIMER_LEFT_2_PENALTY=0.012411
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=CTCTTCTCAACTTCCCTCTCCA
PRIMER_RIGHT_2_SEQUENCE=TAGGGCACAGAGTGAGTTCAAA
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=253,22
PRIMER_LEFT_2_TM=59.988
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.0988
PRIMER_LEFT_3_PENALTY=0.012411
PRIMER_RIGHT_3_PENALTY=0.086339
PRIMER_LEFT_3_SEQUENCE=TCTTCTCAACTTCCCTCTCCAG
PRIMER_RIGHT_3_SEQUENCE=TAGGGCACAGAGTGAGTTCAAA
PRIMER_LEFT_3=36,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=59.988
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.1539
PRIMER_LEFT_4_PENALTY=0.067516
PRIMER_RIGHT_4_PENALTY=0.086339
PRIMER_LEFT_4_SEQUENCE=TCCCTCTCCAGAACCATTCTAA
PRIMER_RIGHT_4_SEQUENCE=TAGGGCACAGAGTGAGTTCAAA
PRIMER_LEFT_4=47,22
PRIMER_RIGHT_4=253,22
PRIMER_LEFT_4_TM=60.068
PRIMER_RIGHT_4_TM=59.914
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_03591 Ps_contig_mira-and-tgicl-ass_franssen_30473
SEQUENCE=AGAACTAATTAACATGAACTCGAGTCAATACTTTATAAATATACAGTAACCATAGTAAAAAAGATACAGATAATAAGATAATAAAAAAGATACAGAAACGATAATAAAAACTACCCAAACATAAAACTCGCAGCTCTTCCTAGCAAAACATAGATCCCTTTGTGAGTCATAAACCCTCTTAGATCAAACAATATTAAATTACAACACAGTAAAAAATAAGTAAAAATAAAGCCACAATACTGCAATAAAAAAGCCCAGTTTGAAAAGGACATGCTGTTGTGGTGAAGCCAAGAGAAATGTGATGATGCACATGAATGCCCCTGAAGGCAAGCAACTGGTGAAAAGCCAATGCATCAACGATAGTACATAAGCTGTTGTCTTGACGAATTACCAGTATATCCATCATACATGGCTTGAGATGCTGCAGAGTTAATGCCCATTGAAGCGGGAGCTAGCCCGTGCTGTCCAGACATTTGAAGAAGAGCTTGTCCGGAAAGGCCAGATACAGCGGCCTGACTCAGGGCCAGCTGGCGCTGATAAGCCAACAACTCGGCGGGAGAATAGCCTTGGTTCATCCCTGCAGTAGGAAGTATTTGATATGGTTGTGCAGCCGGAGGAGGTAATGGATTTGAAGCTGTCCCAGGAGGAGTTGGTTTGCTACCCCATGAACACTTCATCGTCTTCCCACGAACTGGTCTTCCATTAGCCATCTGAATAGCCAGTGCTGCTTCCTCGTGAGTATTGTATCTGACAAATCCAAAACCCTTATCCCTCTGAACTCGGACCTCCTCAAGTACCCCAGCTCCCAATGCATGGAATTGACAATGGAGTTCAGCTTGTGTTACATCATGGGGAAGGTTGCCAACATAAACTGTGGTGTATGAAGGATTATTTTCAGGAGCATCCTCATTGCTGTTATCTTGACCACCATCTGAAGACCCATTTGTAAGCACAACAGCATTTTGGCTGTCATTGATCTTCTCTTCATTTGAGCTGCCACCAGCACCTTTAGTTGCCCAATTGCATCTTATTTGTCTATTTCCCAGCCATTTACCCGTCATATCATTTATAGCACTTTGGGCATCCTGATGATCACGGAAAGAAACAAAACCATATCCTTTTGAGCGACCAGTTTTGTGATCCCACATAACCCTAGCATCAGAACAAGTAGTATAGACTGAGAAGCATGCAAATAAAGTTGCATCAGTAACCTCTGGACTCAAATCACCAACAAATACATTGAAGTGACCTGATGTATCCTCCCTACTGCTATTGGCATATGCCCAATTCACCTTAAGTGCTTGACCATATAGTTGTCGTCCATGCAATGTCATTATTGCAAGTGCTGCGGATGCTCGATCATGGTAGTCCACAAAACCATAAGAAGACTTTTCTTTTCTTATGAGCTTGCACCCAGCAAGAGGGCCAGCACTCTGAAAAACTTCTGCGAGAAGTTTATCGGTGACATTTACATGAATATTTCCTACATAAACACTACGGCATGCAGAAGCATCAAAACCAGGAGGTAGATTTCCGCTTGGTACAGGCTCCATCTGAGTCATAGCGGCAGCGGCAAGCATGCCAGGGTGATGGTACATCTGCTGTTGCTGAAGCAAAGCTTGCTGCATGAGTGCTTGTTGTTGTTGCTGCTAACTTGGTAGTTTCTTTTGNTNCNAYTANTNCCCCTTCTTACCTAAACCCAAAAAAAACCTACCACCCTTCCCCTCGTA
TARGET=1635,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03592 Ps_contig_mira-and-tgicl-ass_franssen_30481
SEQUENCE=AACTTTATTAAGAACATTATTTTAAGCAAATACTCCACATTACATACACAAAACTATCTAATGTACTAGATAATTGATACTTGATTTGCATGTCTTCTGATTTATATAAACTTATAAGACTTTACACAAGAACAATGAAGAGATAGGATATGCATCCTTCCGCATAAGACATTTTACATGGTAGTAAATACAAGTATCATACTTTGCTCCAAGTCTTGAGAAGTAGAAACAAAAGTATAAATTCATGAATAGTATAACAAATGCTGCATAAATTTATACTATTCATGAAAGAAACGCGGCTGGCTGTAAACTGTAAACACAAAACTGTCATAGAAACTCAAATGTCAAGAGGAAATCAATCATACAACTTGGTAGTTTTCTGTGATCAACTGAGTTCTCTGAACAAATTTGGGAGGATTACCACCCTTAAGTGGTATGATATTCCTAACCTTCAGGTCACTAAATGTCTTAGTTGCTGCAAAGCGAACTGAAATGGGAAAGAATGCTGAAGAATCGGTTTGTGGAACAACAAACTCCAGTGACCCACTGCGATTTGAATTATCAATCAGAAGGACAGACCACTCCAAAATGGAATTCCTAGAGTCATACCTCCATTCTCCATCAACCTGACTAACAGATGGCGCCTCTCGAAGAGCTGGAAGAGGTACTGAAACCACAACATTCTGCAGGTCAAACATTGATGAAGCCTCATATTCAATGCTGACATTAGTTTCATTTCCAGAAGAAGAGGGCCAACAGTTGATTGTCAGTGGCACCATTGACTCATCGGTGCTTTGCATTCTCCACTTTAAAAGACCAACACCTGCAGCATCACTGGCTTGACCAGTGGGGAAAGGCCTATTGGRATCTTTTAGACCTAGTATATAGTCATGGGCAAATAGCTCTTTATTCATGTTAGGGTGTGTCTTGAAAGAGATGGCCTGATTATCACCAGTTTGGACCTGAACTTGAATATGTGCGTCTTCTTGGTTAAGAATTTGCAGAGACAATGTGCCTTGAACATCAAAACTACCAACTCCGCCATCTCGTTTCAGAGTCACATTTAATTTCTCCTCAACAGTTAAAGTGACAGGATCAGTAAGTGGTGGGGCAGCTGTCCGAGACTGTCCAAGTCTTGGCTGAACATCTTCAACAATGACCTCACCTTCTGCTTTCAATGATTCCAAAAACTGATTTGTCTTTTGAGATTTACCAAGCTTCATACCAAGACCCTTTGGCGGAGCAGCGGCAGATGCAGTTGGACGGCCTTTAGGTTTGGTAGAAAAGGAATCAACATCAGTACTCAATCCAAAACCAGTTCCAGTGCTAGATATGCTGAAATCACTGAAGCTATTTTCAATTCTTCCAGAACCCATTGATGTTAACGGACCAAATCCTCCTTTATCACCTCTATTCTTTTCAATCTTGCTTTTATCAATCTCATTGGCTTTCCGCTTCATCACATCCTTAGTTTCATTGATCTTACTTTGCATAACCAGCTTGTGCAGCTTCTCTTCGTGACTTTCCATCTCGCAGTACTGCTTAACTTGTGCAACAGTCACATTTTCCTTGTGCCCAAGCGAGATGACTTCATCAAACGCAAAAATCAGCTCAAAGGCATGTCTGCATATACCCTCTTCGTCAAGGGAATAAGAATATTCAGGGACAAGTTTGGAGAGAAGTCTCAGAGTATCCAAATCTTCCAGTATGTTGCTCTGTTTGTTTGTTACAAGTAGCAGGTACAGAGCTTCTATTGGCTGGTAAACATAGCGCACATTCTCAGTCTCAATATATGTGTGTTGTTTCCCAGTGCCTATCAACTTGGGAAATGCTGCTAGAAGTCCCTCAATTCTTATACGAGACATATCAACAAACTGTCTAGAAACTAGCACTTTACCAGACTTGCCGACAATGGATGCAGCAAGAACGACCATTTTGTTCGATTATAATTGAAATGAGGAAAATTCTTGTTCTAGTGTTGAATTGAATTTGAGCAGAATCTGAGATTCGGTGGTGCGGATCTGATCTGAATCGCAGAGGAGTGAAGAAGAAAAGAGAGTGATGGATCAGCGTCACGGCGAAGACGATCCGAAACAAAAT
TARGET=1717,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03593 Ps_contig_mira-and-tgicl-ass_franssen_30495
SEQUENCE=CGGGTTACCTTACTYACNNNTTTTCTCTCCACTTTCCACCTCTTCTTCTCCAACCCAATCATGAACAAAATAAAACAATTCCTAGCCAGAATCTCAAACTCCGGCAACAACAACAACAACAAAAAAAAACTCATCCTCACCCTTTTCACAACGGTTCTCATTATAACCGCCATTGTAGCCATTATCGCAACCGCAACCACAACCAAAAACTCAAACAAAACCAACAAAATCACTTCATCATCTCTCTCACTCTCTCACCATTCTCACACCATTATCAAATCAGCTTGCACCACCACACTCTACCCCGAACTYTGTTTCTCCGCCATCTCCTCTGAACCAAACATAACTCACAAAATCACCAACCACAAAGACGTTATCTCAGTCTCTCTCAACATCACAAAAAGAGCAGTCGAACACAACTTCTTCACCGTGGAAAATCTTCTCACGTTAAAAAACCTCACCGGGAGAGAGAAAATCGCTCTTCATGATTGCTTAGAAACCATTGATGAAACGCTAGATGAACTCAAAGAAGCTGAGGAAGATCTTGTGTTATATCCAGCTAAAAAGACAGTTTATCAACATGCGGACGATCTCAAAACGCTGATAAGCGCCGCCATTACTAACCAGGTAACTTGCTTGGACGGTTTCTCGCACGACGGTGCTGATAAGAGAGTGAGAAGGTTCTAGAAGAAGGACAAGTCCACGTGGAGCATATGTGTAGTAATGCATTAGCCATGACGAAGAACATGACGGATTCAGACATAGCAGAATTTGAACAGAAAAACATGTTGAAAAGTGAAAGAAACGGTAGGAAATTATTGGAGGAGGAGAATGGAGTGAAGTGGCCGGAGTGGATATCGGCGGGGGATAGGAGGCTGCTGCAAGGGGGAGCAGTGAAGGCGGATGTGGTGGTCGCGGCGGATGGGAGTGGGAACTTTAAGACGGTGTCGGAGGCGGTGGCGGCTGCTCCTTTGAAGAGTAGTAAGAGATTTGTAATTAAGATAAAAAGTGGGGTGTATAAAGAAAACGTGGAGGTGCCAAAGAAGAAGATAAATATAATGTTTTTGGGAGATGGTAAAACTAGCACTATTATTAGTGGTGATAGAAATGTTGTTGATGGAAGCACTACTTTTCACTCTGCTACTGTCGCCATAGTAGGAGCAAATTTTCTAGCAAGAGACATAACATTCCAAAACACAGCTGGTCCAGCAAAGCACCAAGCAGTGGCCCTAAGAGTTGGTGCAGACCTCTCAGCCTTCTACAACTGCGACGTCATAGCCTACCAAGACACACTCTATGTCCACAACAACAGACAATTCTTCGTCAACTGTTTCATTTCCGGAACCGTCGATTTCATCTTCGGCAACGCAGCGGTCGTCTTCCAAAACTGCGACATTCATGCAAGACGTCCAAACTCAGGACAGAAGAACATGGTCACTGCACAAGGAAGGGTTGATCCTAACCAAAACACTGGAATTGTGATACAGAAATGTAGGATAGGTGCAACTAAAGATTTGGAGGGTGTGAAGAGTAGTTTTCCTACTTATCTTGGAAGGCCTTGGAAGGAGTATTCAAGAACAGTGATTATGCAAAGTGTTATTAGTGATGTTATTGAACCTGTTGGATGGCATGAATGGAATGGAAATTTTGCTTTGGATACTTTGGTTTATAGGGAATATTTGAATACTGGTCCTGGTTCTGGGACTTCTAAGAGGGTGACATGGAAAGGGTTTAAGGTTATTAGTAATGTTGCTGAGGCACAAAGTTTTACTGCTGGGAATTTTATTGGTGGGTCTAGTTGGTTGGGCTCTACTGGGTTTCCTTTTTCTCTTGGGCTTTAAATTGGGCCCATATAGTTTTTGTTTTCATTTTTATTTTGTTATTTATTAAGTGGGAAAGAGTAACAAAGTTGACCAAATAAAGGTATATAAAGAGAGCAAAAAATGGAAACAGTGAGGGAGATATTGTTGCTCAAGAGGAAGCTTTTTAGTTTGGTAAAAGGCCATGGTCTATATGTGGCTTGGCCTAAATTATTGTGATATTGGTATTGTTTACATGGAAATAAATGTATTTTTGATTTATTAAGATTTTGGTTTGATACCRGAATGTAACTTTG
TARGET=105,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03594 Ps_contig_mira-and-tgicl-ass_franssen_30497
SEQUENCE=TTGCGTGTCTCAGAAGCAGTGGTATTCTAACGCAGAGTAGTCGGGCAGTGTTTTCTGCTCATAAAATTCCAAACCCTCTCTTTTCTTCCACTGCTCTAGATTCGTCGCTCCATGATTAAGCCATTCCACCATTCTCTCAGGAACAGATCGTGAAACTCTGAATTTGAAAAGTAGAAATGGACGATGCTTTTGATGATGAAGGTGGCCCGTCTCTTACAATCAACGAGTACCTTGAGGAAGTTGAGGAACGGGAGCTGGAAGCTGATTTGGTTTTAGGGGGGGATGATGGAAAAGAGTGTACCTATAGCAAAGGTTATATGAAAAGGCAGGCAATTTTCTCCTGCATCACCTGCACCCCAGATGGGAATGCTGGAGTTTGCACTGCTTGCTCGTTGTCTTGCCATGATGGTCATCAGATTGTGGAACTATGGACAAAAAGAAACTTCAGGTGTGATTGTGGGAATTCAAAGTTTGGTGAATTCTACTGCAAGATTTTCCCAAGTAAAGATGTTGAGAATGTTGAGAATTCATACAATCACAACTTTAAAGGTTTGTATTGCACATGTGCCCGTCCTTATCCAGATCCAGATGCTGAAGAACAAATAGAGATGATACAGTGTTGTTTGTGCGAGGATTGGTTTCATGAGGAGCATCTTGGTCTCGAGTCTCTTGATGAGATTCCCAGAGATGATGAAGGGGAGCCGCTTTATGAGGATTTCATATGTAAGGGATGCTCTGAAGTATGTTTCTTTCTGAAATTATATCCTGAAGTAATATTGGTGGCTGAGAAGCAGCCAAATGCTACTGTTCAAGCAAGCAAGGACAAAGGTATTTTGGAAGTTGAGCCTTCAACTTGCGGGTCTGAAAAGCCACTGGGTGATACTTCATACAATTCCCCTAAAATGGATGAGGCACAAGCTTCTGTTAATTCTGAATCTGTATCTGATGGGAAGGGTCTACCTCAGGGAGGAAGTTGTAATGGCAATACTGCTTTAAGTAAATGCACAGAAGGCACCAATTTGAATGTTAATTGTCTCCTTGGTGTGAACATCATTGCTACTTCCCCAGTTAAACATGATAAACCAATGTTTCTTTCGAAAAATTGGAGAGATGCTCTATGCAAATGCAACAATTGTTTGGAATATTACCAGCAGAAGCGAATTGCTTTTCTTCTTGACAAAGATGACTCAATTGTGGAGTATGAGAAAATGGCCAAGCAAAAAAGAGAACAAAAGTTGCAGGAACTGGAGGGTGCTGAGTTAAGTTTTTTTAATAAACTTGGGCACGTAGAGAAAGTTGAGATCTTGAAGGGCATTGAAGAGATGAAGGATGGGCTTCGTTCTTTCCTGGAATCCGCAGACTCCTCCAAGCCAGTTACTGCTGCTGATGTCCATCAGTTTTTCGACGACATTAAGAATAAGCGCCGCCGTATCGAATGATTTAATGTTGTTTTGGTAGTTTACAGGCGTGTTGGCCCTAATTTGCAGACTGTTGAGTTTATTTGTGTAATTTGATGTTCACAAATGCAATGCCCTACTTGTATGAACTCTTTGACTCTGAATGTGCTTCAGCTTCTATAAAGCCAACTTAATGATTTCTTAATGTTGCATATTCTGAATGCAGTCTTGATTGAAAGTTNCT
TARGET=810,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1315
PRIMER_LEFT_PENALTY=0.023267
PRIMER_RIGHT_PENALTY=0.108206
PRIMER_LEFT_SEQUENCE=GCATCTTGGTCTCGAGTCTCTT
PRIMER_RIGHT_SEQUENCE=ATGAAGTATCACCCAGTGGCTT
PRIMER_LEFT=649,22
PRIMER_RIGHT=887,22
PRIMER_LEFT_TM=60.023
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.1400
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.108206
PRIMER_LEFT_1_SEQUENCE=ATTGGTTTCATGAGGAGCATCT
PRIMER_RIGHT_1_SEQUENCE=ATGAAGTATCACCCAGTGGCTT
PRIMER_LEFT_1=633,22
PRIMER_RIGHT_1=887,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=0.2039
PRIMER_LEFT_2_PENALTY=0.095684
PRIMER_RIGHT_2_PENALTY=0.108206
PRIMER_LEFT_2_SEQUENCE=ATACAGTGTTGTTTGTGCGAGG
PRIMER_RIGHT_2_SEQUENCE=ATGAAGTATCACCCAGTGGCTT
PRIMER_LEFT_2=611,22
PRIMER_RIGHT_2=887,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.2669
PRIMER_LEFT_3_PENALTY=0.257927
PRIMER_RIGHT_3_PENALTY=0.008930
PRIMER_LEFT_3_SEQUENCE=TAATATTGGTGGCTGAGAAGCA
PRIMER_RIGHT_3_SEQUENCE=CCATTACAACTTCCTCCCTGAG
PRIMER_LEFT_3=771,22
PRIMER_RIGHT_3=981,22
PRIMER_LEFT_3_TM=59.742
PRIMER_RIGHT_3_TM=59.991
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=0.2704
PRIMER_LEFT_4_PENALTY=0.257927
PRIMER_RIGHT_4_PENALTY=0.012470
PRIMER_LEFT_4_SEQUENCE=TAATATTGGTGGCTGAGAAGCA
PRIMER_RIGHT_4_SEQUENCE=GATGTTCACACCAAGGAGACAA
PRIMER_LEFT_4=771,22
PRIMER_RIGHT_4=1051,22
PRIMER_LEFT_4_TM=59.742
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_03595 Ps_contig_mira-and-tgicl-ass_franssen_30507
SEQUENCE=TTCGGGGTACGGTAAGTAACTATTTGTGACACGGTGCGGTTGTGGCACGAACGAACGAACATAAACATAAACGTTGGTTGGTGTTGTCGATGTTATTGTTATCCACAAAGAAGAAAAACAACAACACAAGGCTTAGAGTGAGTGCCATGGCAACATCTTTCTCTCCCACAACACCGCTCCGTTCCATCTTCCAAAACCATCCCAAACCTCTTCTTCTCTCCCACTCAGATTCCTCTTACAGCGTCAAACGCTCTTCCACCTATGCCTCTCTAAACCTCCCTCGCAACTTCACACTCTCTAGGAATGGAAAACCTCTCCTCTCTAAAGGATTCTTGACCTTGCATAGAAGAAAGGGAATTGTGAGGGCTGCTACTATTGAAGAAATTGAAGCTGAAAAAGCATCTATTGAGAAAGATGTTAAAAACAGAATGGAAAAGACTCTTGATAATGTTAGGTCTAATTTCAGTTCCATAAGGACCGGGCGAGCAACCCCGACTATGCTTGACAAAATTGAGGTGGAATACTATGGATCTCCAGTTAGCTTGAAGAGCATTGCTCAAATAAGCACTCCTGATGCCAGTTCTCTGTTGGTACAGCCATATGACAAATCAATCTTGAAGTCTATTGAGAAAGCCATAGTTAACTCTGATGTTGGTTTGACTCCAAATAACGATGGAGACGTGATAAGGTTGAGTATCCCTCAGTTGACATCTGATAGACGAAAGGAACTAACGAAGATAGTGTCTAAACAAGTTGAAGAAGGGAAGGTGGCTTTGAGGAACATAAGAAGAGATGCTTTGAAAGCTTATGAAAAACTTGAAAAGGAAAAAAAGCTCTCAGAAGATAATGTGAAGGACTTGTCTAGCGACTTGCAGAAGCTGACAGATGATTATATAAAGAAGGTTGATGTGATCTACAAACAGAAGGAAAAGGAGTTGCTGACCGTTTGATCCATTGACACAAAAAAGTAATTGTGTTTGAGTTGTATCTTAAGATCAAATTTTGTGTCTACTTTGCTAAGTTTGATCGTTGATGCATGTTTATTTGCTATAGAGAGACAATGTTTTTTCTGTTAAATAGATGCTTTTTCAAATTGTAAAAAGTGAGTTCATGCATGTAAATTTGGATTTATTTACAATTGCATCATTGAAGCTTACAAGGGTTTAAATACTCCAAATTTGTGAAAACAAAAAAGTAAATTGGGAGAAGAAGACTTCGAAGAGTTTTGGAG
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9772
PRIMER_LEFT_PENALTY=0.961984
PRIMER_RIGHT_PENALTY=0.015253
PRIMER_LEFT_SEQUENCE=TAACTATTTGTGACACGGTGCG
PRIMER_RIGHT_SEQUENCE=TGACGCTGTAAGAGGAATCTGA
PRIMER_LEFT=16,22
PRIMER_RIGHT=245,22
PRIMER_LEFT_TM=60.962
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=1.1447
PRIMER_LEFT_1_PENALTY=0.961984
PRIMER_RIGHT_1_PENALTY=0.182682
PRIMER_LEFT_1_SEQUENCE=TAACTATTTGTGACACGGTGCG
PRIMER_RIGHT_1_SEQUENCE=GGAATCTGAGTGGGAGAGAAGA
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=60.962
PRIMER_RIGHT_1_TM=59.817
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=1.1447
PRIMER_LEFT_2_PENALTY=0.961984
PRIMER_RIGHT_2_PENALTY=0.182682
PRIMER_LEFT_2_SEQUENCE=TAACTATTTGTGACACGGTGCG
PRIMER_RIGHT_2_SEQUENCE=GAGGAATCTGAGTGGGAGAGAA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=234,22
PRIMER_LEFT_2_TM=60.962
PRIMER_RIGHT_2_TM=59.817
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=1.1772
PRIMER_LEFT_3_PENALTY=0.961984
PRIMER_RIGHT_3_PENALTY=0.215198
PRIMER_LEFT_3_SEQUENCE=TAACTATTTGTGACACGGTGCG
PRIMER_RIGHT_3_SEQUENCE=AGAAGAGGTTTGGGATGGTTTT
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=213,22
PRIMER_LEFT_3_TM=60.962
PRIMER_RIGHT_3_TM=60.215
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=1.1772
PRIMER_LEFT_4_PENALTY=0.961984
PRIMER_RIGHT_4_PENALTY=0.215198
PRIMER_LEFT_4_SEQUENCE=TAACTATTTGTGACACGGTGCG
PRIMER_RIGHT_4_SEQUENCE=AAGAAGAGGTTTGGGATGGTTT
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=214,22
PRIMER_LEFT_4_TM=60.962
PRIMER_RIGHT_4_TM=60.215
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_03596 Ps_contig_mira-and-tgicl-ass_franssen_30508
SEQUENCE=AGTACGCGGGACGCATATAGAAGNGATGAGTTTGTTACGTGCATAGTGGATTGCATTCTGTTACGATTCATTCATTCATAGATTGAAAAAGTTAGTTACTATTGAACTGAATTGGAAGAAGAAATGGATCACATCGCCGATGCACAACGCACCGATTTGATGACCATAACCCGTTTCGTTCTCAACGAACAATCAAAGCACCCTGAATCACGCGGCGATTTCAGCATCTTGCTCAGTCACATTGTTCTTGGATGTAAATTCGTTTGTTCCGCTGTTAACAAGGCTGGTCTTGCTAAGCTCATTGGACTTGCTGGAGAGACCAATGTGCAGGGTGAAGAACAGAAGAAGCTGGATGTTCTTTCCAATGATGTATTCATCAAGGCTTTGAAAAGCAGTGGCAGAACATGCATCTTGGTTTCTGAAGAAGATGAGGAGGCAATATTTGTGGATCCCTCGCAACGTGGAAAGTACTGTGTTGTTTTTGATCCATTGGATGGCTCCTCTAACATTGATTGTGGTGTTTCCATTGGAACTATTTTTGGGATTTATATGATGAAAGATAACCATGCACCAGTCATAGAAGATGTCCTGCAACCTGGGAAGAACATGTTGGCAGCTGGTTATTGTATGTATGGAAGCTCTTGCACGCTTGTGATAAGCACCGGAAGTGGTGTTAATGGTTTCACCCTTGACCCTTCTCTCGGTGAATTCATCTTAACTCACCCTGACATTAAGATCCCAAAGAAAGGAAAGATTTATTCAGTGAATGAAGGGAATGCCAAGAACTGGGATGGCCCTACTGCCGCTTATGTGGAGAAATGCAAGTTTCCCACAGATGGTTCATCAGCAAAGTCTCTAAGATATATTGGAAGCATGGTAGCTGATGTTCATCGCACATTGCTTTATGGAGGTGTCTTTTTGTACCCTGCTGACAAAAAGAGTCCAAGTGGAAAACTTCGTGTATTGTATGAAGTCTTCCCAATGTCCTTCTTGTTGGAACAGGCCGGAGGACAGTCTTTCACCGGCAAGCAAAGGGCACTTGATTTAATTCCAACAAAACTTCATGAACGGTCTCCCATATTTCTTGGTAGCTATGACGATATCGAAGAAATCAAAGCTCTATATGCTGCAGAAGAGGGTAAATAAAAGGAACCTCAAAAGCTTCCTTCTGTTTTTCATGCAAGCATTATCCAATTGTTAGGCTAACAAGTGCCTATAACAGTTAAAACTTGTTTACTTGCTCATGTATATGCTATAAAACTTCTTATTGAGCTGCTGCTTGCCATGGTTATAGTTTACATTTACTTGTTTTGGTAACAAAGGTTATAGTTTATATTAATGGATAAACAATGTTAGAAATT
TARGET=66,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3851
PRIMER_LEFT_PENALTY=0.362762
PRIMER_RIGHT_PENALTY=0.022302
PRIMER_LEFT_SEQUENCE=AGTGGATTGCATTCTGTTACGA
PRIMER_RIGHT_SEQUENCE=TGACTGAGCAAGATGCTGAAAT
PRIMER_LEFT=44,22
PRIMER_RIGHT=238,22
PRIMER_LEFT_TM=59.637
PRIMER_RIGHT_TM=60.022
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.4113
PRIMER_LEFT_1_PENALTY=0.362762
PRIMER_RIGHT_1_PENALTY=0.048570
PRIMER_LEFT_1_SEQUENCE=AGTGGATTGCATTCTGTTACGA
PRIMER_RIGHT_1_SEQUENCE=AACGGGTTATGGTCATCAAATC
PRIMER_LEFT_1=44,22
PRIMER_RIGHT_1=174,22
PRIMER_LEFT_1_TM=59.637
PRIMER_RIGHT_1_TM=59.951
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.4271
PRIMER_LEFT_2_PENALTY=0.404834
PRIMER_RIGHT_2_PENALTY=0.022302
PRIMER_LEFT_2_SEQUENCE=TTACGTGCATAGTGGATTGCAT
PRIMER_RIGHT_2_SEQUENCE=TGACTGAGCAAGATGCTGAAAT
PRIMER_LEFT_2=34,22
PRIMER_RIGHT_2=238,22
PRIMER_LEFT_2_TM=60.405
PRIMER_RIGHT_2_TM=60.022
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.5538
PRIMER_LEFT_3_PENALTY=0.531474
PRIMER_RIGHT_3_PENALTY=0.022302
PRIMER_LEFT_3_SEQUENCE=TGCATAGTGGATTGCATTCTGT
PRIMER_RIGHT_3_SEQUENCE=TGACTGAGCAAGATGCTGAAAT
PRIMER_LEFT_3=39,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=60.531
PRIMER_RIGHT_3_TM=60.022
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=0.5793
PRIMER_LEFT_4_PENALTY=0.557021
PRIMER_RIGHT_4_PENALTY=0.022302
PRIMER_LEFT_4_SEQUENCE=GNGATGAGTTTGTTACGTGCAT
PRIMER_RIGHT_4_SEQUENCE=TGACTGAGCAAGATGCTGAAAT
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=238,22
PRIMER_LEFT_4_TM=59.443
PRIMER_RIGHT_4_TM=60.022
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_03597 Ps_contig_mira-and-tgicl-ass_franssen_30511
SEQUENCE=AAATAGATGACCAGCTACTTTGCGACATAGATATTGAACAAGAAGCTCTTCATCGGTTGGGTAAAATCGAAAACCAGGTGGTAAACTCAATTGTGAAAGAGTGTCGTTTTCTTGAACTCCCATTATTATTATTTACCACAAAGAAACAATGGAGTTATTGAGTTGAAGAGAAGATGAAGAAGATTTTGGTGGTGAAGTTTTTTTTTGCTTAGAGAGAAGAAATGTTATCTCTTTGAATTTTCTTTTC
TARGET=122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4856
PRIMER_LEFT_PENALTY=0.039178
PRIMER_RIGHT_PENALTY=1.446387
PRIMER_LEFT_SEQUENCE=TAGATGACCAGCTACTTTGCGA
PRIMER_RIGHT_SEQUENCE=CACCACCAAAATCTTCTTCATC
PRIMER_LEFT=3,22
PRIMER_RIGHT=193,22
PRIMER_LEFT_TM=60.039
PRIMER_RIGHT_TM=58.554
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=1.6302
PRIMER_LEFT_1_PENALTY=0.183834
PRIMER_RIGHT_1_PENALTY=1.446387
PRIMER_LEFT_1_SEQUENCE=TCTTCATCGGTTGGGTAAAATC
PRIMER_RIGHT_1_SEQUENCE=CACCACCAAAATCTTCTTCATC
PRIMER_LEFT_1=46,22
PRIMER_RIGHT_1=193,22
PRIMER_LEFT_1_TM=60.184
PRIMER_RIGHT_1_TM=58.554
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=148
PRIMER_PAIR_PENALTY_2=1.6942
PRIMER_LEFT_2_PENALTY=0.247794
PRIMER_RIGHT_2_PENALTY=1.446387
PRIMER_LEFT_2_SEQUENCE=GTTGGGTAAAATCGAAAACCAG
PRIMER_RIGHT_2_SEQUENCE=CACCACCAAAATCTTCTTCATC
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=193,22
PRIMER_LEFT_2_TM=59.752
PRIMER_RIGHT_2_TM=58.554
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=1.8310
PRIMER_LEFT_3_PENALTY=0.384647
PRIMER_RIGHT_3_PENALTY=1.446387
PRIMER_LEFT_3_SEQUENCE=TCGAAAACCAGGTGGTAAACTC
PRIMER_RIGHT_3_SEQUENCE=CACCACCAAAATCTTCTTCATC
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=193,22
PRIMER_LEFT_3_TM=60.385
PRIMER_RIGHT_3_TM=58.554
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=128
PRIMER_PAIR_PENALTY_4=2.0742
PRIMER_LEFT_4_PENALTY=0.627767
PRIMER_RIGHT_4_PENALTY=1.446387
PRIMER_LEFT_4_SEQUENCE=CTCTTCATCGGTTGGGTAAAAT
PRIMER_RIGHT_4_SEQUENCE=CACCACCAAAATCTTCTTCATC
PRIMER_LEFT_4=45,22
PRIMER_RIGHT_4=193,22
PRIMER_LEFT_4_TM=59.372
PRIMER_RIGHT_4_TM=58.554
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_03598 Ps_contig_mira-and-tgicl-ass_franssen_30515
SEQUENCE=GCAATATATATATACACTTTCTCAATGTCGGGGAAAGTCAAATCCATTTATTCTTCTCCTCAACTAATATTCTCCTCATTCACAAAAATGCCGAAAGATAGAACTATTGGTGTAGCTTTGGATTTCTCCAAAAGCAGCAAGAATGCTCTCAAATGGGCTCTTGAAAATTTAGCTGATAACGGTGACAACATTTACATCATCCACATCAGCCAAGATTCCGTTGATGAATCTCGCAATCAGCTATGGTCCAAATCTGGTTCTCCTTTGATTCCTTTGAAAGAGTTCAGAGAACCTGAGATCATGAACAAGTATGGTGTTCAGATTGATATTGAGGTTCTTGATTTGCTTGACACTTTTTCAAGACAAAAAGAGGTGAATGTTGTTACAAAAGTGTATTGGGGTGATGCAAGAGAGAAACTTTTGGATGCTGTTGAAGATCTGAAGCTGGATTCTCTTGTTATGGGAAGCAGGGGTCTCAGCACAATTCAAAGGATCTTATTGGGAAGTGTAAGCAACTTTGTGATGGCTAATGCTCCTTGCCCTGTGACTATTGTCAAGGACAATATCAGCACCAGCAGCAAGTAAAGACGATGATCAATTTGCGATGGCATTTAAGTATGGAATATTCTGTTTCTTGCATTGAACTTGAACTCTTGTTGATAATCTTCTGTTTCCTTATTTCGGATTTATATTATGTTATGACTATATCTATCTGGTTTACTTTATTGAAAGAATAATAATGTAGTTTTAGTTTTATCATCATCCATATATTGCTATCCATATTGTGCCRACC
TARGET=4,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03599 Ps_contig_mira-and-tgicl-ass_franssen_30516
SEQUENCE=TTCAATGCTAATTTAATCTTCTTAGACAACTTTCCTTAAAAGCATTGCCCTACGCAACGACGTCGTTNACTCGCGGGCATGGCATTGTAAGCAGTAACCGACGTCGTAGCGGCCGTTGCATTGGGTATCTGTCGATCACCGTACCGGAGAAACGAGACCAGAAGAATCAGAACTGAAAAGTTCACAAAAAGCCTTCGAATGGCTCCACCCAAGTCTAGAGGAGCCACTCGGAGCAGCAACGCAAGGATTCTTCTCTTTTCCTTTCTCCTCGCTCTCCAATACGGCGCTCAACCTTTGATTTCCAAACGCTGCATCAGCCGTGAAGTTATTGTGACTTCATCCGTTTTGGCATGCGAGGCTGCAAAGGTTATATTTGCATTGTACTTCATGACAAAAGAAGGTAGTCTGGGAAGAATGTATAAAGAATGGACTTTGGTCGGTGCATTGACTGCATCAGCACTTCCTGCGGCTATATATGCACTGCAAAATAGTTTGTTGCAAATTTCTTACAAGAATCTTGATTCACTCACATTCTCAATTCTGAATCAGACAAAAATATTTTTCACTGCATTATTTACATATTTCATGTTGAGGCAAAAACAAACAATTCAACAAATTGGAGCCTTGTTCTTGTTATTAGCCGCAGCAGTTTTACTAAGTGTTGGTGAAGGTTCTAACAAAAGTTCTACTAGTGCTAATGCTGATCAAGTTTTATTCTATGGAATAGTTCCTGTATTAATTGCTTCAGTGCTTTCTGGACTAGCTTCTTCCCTGTGCCAATGGGCTTCTCAGGTTAAGAAACACTCATCATACCTAATGACTGTAGAAATGTCTATGGTTGGAAGTATATGTTTGCTTGCCAGTACATTGAAATCTCCAGATGGGGAAGCTATGAGACGTCATGGATTTTTTCATGGTTGGACTGCTCTAACTTGGATCCCAGTAATTTTGAATGCCCTTGGTGGAATTCTTGTTGGTTTAGTTACAAGCCATGCTGGCGGTGTTCGAAAGGGATTTGTCATTGTCTCAGCTTTACTCGTTACAGCATTACTACAGTTCATTTTTGAAGGAAAACCGCCTTCATTATATTGCCTTTTGGCTCTTCCACTAGTGGTCTGTAGCATTTCAATATATCAGAAATACCCCTATCTGGTTAAGAAGAAGGAGTCATAAAACTTGACTGCCACAGTACGGGGAAGTTACTTCATTAGTTCCCCAACGTAGCGCCTTTGCTATACACAATATATAAGCATATTGTAAATGATAGAAGGCAAGCATTGTTTTCAATGTCTGCTCGAGCTCAATAGAATGAGGATGTTATATATACTTTTATTTTTCTATTCTCTTTTTTTGCTCTGGAACGTTTGAAGAGCCATTTATTTTGGTCTTTCTTCAATGTGTGATAGAGGTCCGAAAGTGCGCTTCATTTCTTCAAATATATATATATATATTACTGTTACGA
TARGET=1436,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03600 Ps_contig_mira-and-tgicl-ass_franssen_30518
SEQUENCE=GGGGNGGATTCCATCAAAATCAAGAGCGACCAATTCTTGCTCAAAAACATCATCATCATCATCATTATCCATCATGGAGAACAACAACAACAACCACAACGAACACCAACAAACCCAATATCCAACACCGCAACCCACGGCGGGCACTCCCCCGCCATTCCAACACCTCTTACAGCAACAACAACAACAGCTCCAAATGTTCTGGTCCTACCAAAGACAAGAAATCGAACATGTCAACGACTTCAAAAACCACCAACTTCCATTAGCACGCATTAAGAAAATCATGAAAGCCGACGAAGACGTTCGCATGATCTCCGCTGAAGCACCTATCCTTTTCGCTAAAGCTTGCGAGCTTTTCATTCTCGAACTCACCATTCGTTCTTGGCTTCATGCTGAAGAGAATAAACGACGAACCCTGCAGAAAAACGACATCGCTGCTGCTATTACGCGAACTGATATTTTTGTATTTTTTGGTTGATATTGTTCCGAGAGATGAGATTAAAGATGAAGCTACCATTGTTGGCGCTACTGCTAGTGGTGTTCCTTATTATTATCCTCCTATGGGACAACCTGCGGGGATGATGATTGGTCGTCCGGCGGTTGATCCGGCTACTGGTGTTTATGTTCAGCCTCCTTCTCAGGCTTGGCAGTCCGTTTGGCAGACTGGTGCTGATGATGGCTCTTATGCTGCTGCCGCCGGTGGTGGTACTAGTGGTCACCACAATGTCGATGGCTAGAGCCTGAGTGACCACACCGCTGATGATGGATTATCAAGCCGCGCTGGACTTTTGCGATGTTGCATGGAATTTTTTTATTGCTTGCAATTTAGAAATGGTTTTGTTAACTAAATTTGTATGGGGTGGCATCAAGAACATGTTAACTTGATGATCAACAGCATGATTTAACTGTGTATGTACAAAAAGTAAAATGGAACAATGATCCTTCTGTGCAATGTGTGAAACGTGTTAATCATCTGCTAACAGATTTTAGGCTGTATTTTTGCAGATGTTTCCTAATTCCTACGATACTAATTGTATGTTGGTGTTCGGTGCAAGTTGGGTGGTTAATTTCCACACCGCCTATGAACGAATATAATGTTGGATTTATAAAAGTGGTTTGTATATCTTGTATCGTAAGTTANTGGTGGAGATGTGGTGTTTCCCTTTTTGTTCG
TARGET=48,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0887
PRIMER_LEFT_PENALTY=0.084327
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_SEQUENCE=GTAGGACCAGAACATTTGGAGC
PRIMER_LEFT=5,22
PRIMER_RIGHT=210,22
PRIMER_LEFT_TM=59.916
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.1365
PRIMER_LEFT_1_PENALTY=0.084327
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_1_SEQUENCE=GTGGTTTTTGAAGTCGTTGACA
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=252,22
PRIMER_LEFT_1_TM=59.916
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.1412
PRIMER_LEFT_2_PENALTY=0.084327
PRIMER_RIGHT_2_PENALTY=0.056850
PRIMER_LEFT_2_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_2_SEQUENCE=AGTTGGTGGTTTTTGAAGTCGT
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=59.916
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.2203
PRIMER_LEFT_3_PENALTY=0.084327
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_3_SEQUENCE=GGTAGGACCAGAACATTTGGAG
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=59.916
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=0.2343
PRIMER_LEFT_4_PENALTY=0.084327
PRIMER_RIGHT_4_PENALTY=0.149938
PRIMER_LEFT_4_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_4_SEQUENCE=TAATGCGTGCTAATGGAAGTTG
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=59.916
PRIMER_RIGHT_4_TM=60.150
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_03601 Ps_contig_mira-and-tgicl-ass_franssen_30518
SEQUENCE=GGGGNGGATTCCATCAAAATCAAGAGCGACCAATTCTTGCTCAAAAACATCATCATCATCATCATTATCCATCATGGAGAACAACAACAACAACCACAACGAACACCAACAAACCCAATATCCAACACCGCAACCCACGGCGGGCACTCCCCCGCCATTCCAACACCTCTTACAGCAACAACAACAACAGCTCCAAATGTTCTGGTCCTACCAAAGACAAGAAATCGAACATGTCAACGACTTCAAAAACCACCAACTTCCATTAGCACGCATTAAGAAAATCATGAAAGCCGACGAAGACGTTCGCATGATCTCCGCTGAAGCACCTATCCTTTTCGCTAAAGCTTGCGAGCTTTTCATTCTCGAACTCACCATTCGTTCTTGGCTTCATGCTGAAGAGAATAAACGACGAACCCTGCAGAAAAACGACATCGCTGCTGCTATTACGCGAACTGATATTTTTGTATTTTTTGGTTGATATTGTTCCGAGAGATGAGATTAAAGATGAAGCTACCATTGTTGGCGCTACTGCTAGTGGTGTTCCTTATTATTATCCTCCTATGGGACAACCTGCGGGGATGATGATTGGTCGTCCGGCGGTTGATCCGGCTACTGGTGTTTATGTTCAGCCTCCTTCTCAGGCTTGGCAGTCCGTTTGGCAGACTGGTGCTGATGATGGCTCTTATGCTGCTGCCGCCGGTGGTGGTACTAGTGGTCACCACAATGTCGATGGCTAGAGCCTGAGTGACCACACCGCTGATGATGGATTATCAAGCCGCGCTGGACTTTTGCGATGTTGCATGGAATTTTTTTATTGCTTGCAATTTAGAAATGGTTTTGTTAACTAAATTTGTATGGGGTGGCATCAAGAACATGTTAACTTGATGATCAACAGCATGATTTAACTGTGTATGTACAAAAAGTAAAATGGAACAATGATCCTTCTGTGCAATGTGTGAAACGTGTTAATCATCTGCTAACAGATTTTAGGCTGTATTTTTGCAGATGTTTCCTAATTCCTACGATACTAATTGTATGTTGGTGTTCGGTGCAAGTTGGGTGGTTAATTTCCACACCGCCTATGAACGAATATAATGTTGGATTTATAAAAGTGGTTTGTATATCTTGTATCGTAAGTTANTGGTGGAGATGTGGTGTTTCCCTTTTTGTTCG
TARGET=80,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0887
PRIMER_LEFT_PENALTY=0.084327
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_SEQUENCE=GTAGGACCAGAACATTTGGAGC
PRIMER_LEFT=5,22
PRIMER_RIGHT=210,22
PRIMER_LEFT_TM=59.916
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.1365
PRIMER_LEFT_1_PENALTY=0.084327
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_1_SEQUENCE=GTGGTTTTTGAAGTCGTTGACA
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=252,22
PRIMER_LEFT_1_TM=59.916
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.1412
PRIMER_LEFT_2_PENALTY=0.084327
PRIMER_RIGHT_2_PENALTY=0.056850
PRIMER_LEFT_2_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_2_SEQUENCE=AGTTGGTGGTTTTTGAAGTCGT
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=59.916
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.2203
PRIMER_LEFT_3_PENALTY=0.084327
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_3_SEQUENCE=GGTAGGACCAGAACATTTGGAG
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=59.916
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=0.2343
PRIMER_LEFT_4_PENALTY=0.084327
PRIMER_RIGHT_4_PENALTY=0.149938
PRIMER_LEFT_4_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_4_SEQUENCE=TAATGCGTGCTAATGGAAGTTG
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=59.916
PRIMER_RIGHT_4_TM=60.150
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_03602 Ps_contig_mira-and-tgicl-ass_franssen_30518
SEQUENCE=GGGGNGGATTCCATCAAAATCAAGAGCGACCAATTCTTGCTCAAAAACATCATCATCATCATCATTATCCATCATGGAGAACAACAACAACAACCACAACGAACACCAACAAACCCAATATCCAACACCGCAACCCACGGCGGGCACTCCCCCGCCATTCCAACACCTCTTACAGCAACAACAACAACAGCTCCAAATGTTCTGGTCCTACCAAAGACAAGAAATCGAACATGTCAACGACTTCAAAAACCACCAACTTCCATTAGCACGCATTAAGAAAATCATGAAAGCCGACGAAGACGTTCGCATGATCTCCGCTGAAGCACCTATCCTTTTCGCTAAAGCTTGCGAGCTTTTCATTCTCGAACTCACCATTCGTTCTTGGCTTCATGCTGAAGAGAATAAACGACGAACCCTGCAGAAAAACGACATCGCTGCTGCTATTACGCGAACTGATATTTTTGTATTTTTTGGTTGATATTGTTCCGAGAGATGAGATTAAAGATGAAGCTACCATTGTTGGCGCTACTGCTAGTGGTGTTCCTTATTATTATCCTCCTATGGGACAACCTGCGGGGATGATGATTGGTCGTCCGGCGGTTGATCCGGCTACTGGTGTTTATGTTCAGCCTCCTTCTCAGGCTTGGCAGTCCGTTTGGCAGACTGGTGCTGATGATGGCTCTTATGCTGCTGCCGCCGGTGGTGGTACTAGTGGTCACCACAATGTCGATGGCTAGAGCCTGAGTGACCACACCGCTGATGATGGATTATCAAGCCGCGCTGGACTTTTGCGATGTTGCATGGAATTTTTTTATTGCTTGCAATTTAGAAATGGTTTTGTTAACTAAATTTGTATGGGGTGGCATCAAGAACATGTTAACTTGATGATCAACAGCATGATTTAACTGTGTATGTACAAAAAGTAAAATGGAACAATGATCCTTCTGTGCAATGTGTGAAACGTGTTAATCATCTGCTAACAGATTTTAGGCTGTATTTTTGCAGATGTTTCCTAATTCCTACGATACTAATTGTATGTTGGTGTTCGGTGCAAGTTGGGTGGTTAATTTCCACACCGCCTATGAACGAATATAATGTTGGATTTATAAAAGTGGTTTGTATATCTTGTATCGTAAGTTANTGGTGGAGATGTGGTGTTTCCCTTTTTGTTCG
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0887
PRIMER_LEFT_PENALTY=0.084327
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_SEQUENCE=GTAGGACCAGAACATTTGGAGC
PRIMER_LEFT=5,22
PRIMER_RIGHT=210,22
PRIMER_LEFT_TM=59.916
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.1365
PRIMER_LEFT_1_PENALTY=0.084327
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_1_SEQUENCE=GTGGTTTTTGAAGTCGTTGACA
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=252,22
PRIMER_LEFT_1_TM=59.916
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.1412
PRIMER_LEFT_2_PENALTY=0.084327
PRIMER_RIGHT_2_PENALTY=0.056850
PRIMER_LEFT_2_SEQUENCE=GGATTCCATCAAAATCAAGAGC
PRIMER_RIGHT_2_SEQUENCE=AGTTGGTGGTTTTTGAAGTCGT
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=59.916
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.1479
PRIMER_LEFT_3_PENALTY=0.143555
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=AACACCAACAAACCCAATATCC
PRIMER_RIGHT_3_SEQUENCE=GTAGGACCAGAACATTTGGAGC
PRIMER_LEFT_3=101,22
PRIMER_RIGHT_3=210,22
PRIMER_LEFT_3_TM=59.856
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=110
PRIMER_PAIR_PENALTY_4=0.1479
PRIMER_LEFT_4_PENALTY=0.143555
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=AACAAACCCAATATCCAACACC
PRIMER_RIGHT_4_SEQUENCE=GTAGGACCAGAACATTTGGAGC
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=210,22
PRIMER_LEFT_4_TM=59.856
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=104
=
PRIMER_SEQUENCE_ID=SSR_03603 Ps_contig_mira-and-tgicl-ass_franssen_30534
SEQUENCE=ATGCAATAAATGACTAGAGTTTTGTGGATATATAAATTTACGCAGAATACGTNCGGGGATATTAACAAGTTATCTCTTTCTCTAAAATGGTTGTGTATATGATGAAGGTTCCAAGTACTTACTCATCAACTAGATTAATACTATTACGTTTTATTTTTTTGTTAGTTTTATCCTATAGAACAAACTCATTGGTGAAACTACCACCAAATGTTACAGTTCCTGCATTCATAGCTTTTGGAGATTCCATCATGGATACTGGAAATAATAACAATATCAAAACTATTGTTAAGTGCAATTTCTCTCCTTATGGTCAAGATTTTGAAGGAGGGATTCCAACTGGTCGATTTTGTAATGGCAAGAATCCTTCAGATTTGATAGTTGAATATTTCGGTATTAAGGAGCTTTTACCAGCTTATTTGGATCCAAATCTGAAACCAAGTGACCTTCCAACTGGTGTATGCTTTGCTTCTGGTGCTTCTGGTTATGATCCTTTAACATCTAAAATTGTGTCGGTGATTTCAATGTCTGATCAACTAGAAATGTTCAAAGAATACATAGTGAAACTCAAAGATGTGGTTGGAGAAAAAAGAAAAAAACTTCATCATAGCCAACACTCTTTTCCTTGTTGTAGCTGGAAGTGATGACCTTGCCAACACTTATTTTACTATTCGCACCCCACAACTTCACTATGATGTTCCTGCTTATACTGATCTTATGGTCAACGAAGCCTCAAAGTTCGTTAAGGAAATATRTTTNATTCTTAATAAATTAAACAATACTAACAACAACAAAATTAATATACATTTTCATATATAATTGAAGGAAATATATCAACTTGGAGCTAGGAGAATTGGAGTGTTCAGTGCAGCACCTATTGGATACTTACCATCACAGAGAACACTAAGTGGGGGTTTATCTAGAAATATCAATGAAGAATACAATGAAGCAGCCAAGTTATTTAACTCCAAACTCTCAAACCAAATGGATTATCTTCACTCTAGTTTGCCTAATAGTAGGGTTATTTATATTGATATTTACACCCCCTTACTTGATATCATTCTAAAGCCTCAAAAATATGGGTATAGAGTTGCAGATAAAGGTTGTTGTGGAACAGGAAAACTAGAAGTTTCAGTGTTATGTAACCCTTTGATTCCCACATGTGATAATAATAAAGAATATGTCTTTTGGGATAGTTATCATCCTACAGAAAGTGTATACAGAACGCTTGTGGGTGAGGTTCTTCCAAAATATAGGGATCGCTTGACAAGATGATGACATCTCATGTTTTCTCATGGCATGTTATAACTATCTATTTATCTTTTTTTATAATTATTATTATTGTTTTGTTTCACAATGATGACTTTAACATGTTTAAAGTGTACAACTGTTCCCATTTTTTTGTGGAGATAATAAAAAGAATTATGTTTAAAGTGTGAAC
TARGET=1329,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03604 Ps_contig_mira-and-tgicl-ass_franssen_30535
SEQUENCE=CTTAGGTCGTTACCTTACCTATAGAACAATAGCAGTCTTTTATTCTTTCTATGTAAATTCATCATTATCACATCATCACAAACTTATGTTGCACATCAAATTCATGCATTTCATGTAGAGCTATTCTAAACCTACACGCATAATACTAACATCAAAGAACAGAAACACTAATGCAATGTGGGAAGAAAAAGAATAGGTCACACAATTGATACACATTAAGACCAACAACAGTAAGAGTAAACTAGAAACACATGAAACAACTTGGAATCTTAATCTACTAGCACCATTCTGCAGTTATCAGCAATAACATCTATGCCTACAGAGTACAAACAAACAAGCATCCTTTTATTCCTGCTTCCTCGAGTCTTTGAGCTGCCCCCTCTTTCTAATGCCCATACAAAACCCGAGGCGCCAAGTATCAACTACGTACTCCGGCAATGAACACGCCACAGCCCAAAGAAGGGTATGTGGCCAATAGGGTGTGCAACGAGGTTCGTATCCTATCCATTTCACACCTGCTTTCGCGTATCCGTCTGTTGATGGAACGAAGAATGAAGATCTCTTGATTGAAGCCATCTTTGTTGCCACATATAAAGGAACCTGACACTGCACATCAATCCCACTCTTCTTGTATTCAACATAGAGACATCTAGAGAATTGATCAATGTACGCTTTAGTGGCAGCATAAACCGCATAAAGTGGATCAGAAGGAATAACAATAGCAGCACCAGAACCAATGTTAACAATCGCTCCCTTCTTTCTCTTCAACATCCCAGGTAAAACAGCATGCGTAACCTTGGTAGTTCCCACAACATTCACCTTAATCAAATTCTTCAGAAGCTCCTCATCCAATTCATGGAAGAATCTCGCATAAGGGTAAGAAATCCCCACGTTGTTAACCAACACCCCAATATCCAACCCCTCAATCGCTTCACGAATTCTATCCACACCCTCGTCGAGATCACCGGTGAAATCAACGACAACGGTTTTAACCTCGGTTTTTCCGAACTTGGCTATGACAGAATCTGAAACGTCTTTGAGTTTGTCGGGGTTGCGACCGACAAGGATTAGGTTCAAGCCTTTACGAGCGAGTTCAAAGGCGAAGCTTTTTCCAATGCCGTCTGTGGGTCCGGTAACCAGTGCCCAAGATCCGTACTTTTTGAGGTTTTTGGGTTGTCTGAGAAAATTAACATAGACCCAGTTGAGGAATGTTAGAGTGAATCTGACAATGGTGATGAGACCGAGTGAGAAGAGAAGGAGGAACCAGAGAGGTTCGGATTTGAGTTTACTGATTATGCAGCAATCCATATTGAGGGTGGAAGGAAGAAGAAGAAGAAAATGGTAACAGTTGAGTTGTGAGGCGAAAAAGAGAGAGGGGTTTAATTATTTATATCAAGAANATAAGAGAGAGTAAGATT
TARGET=326,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0845
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.080887
PRIMER_LEFT_SEQUENCE=CACTAATGCAATGTGGGAAGAA
PRIMER_RIGHT_SEQUENCE=ATTGCCGGAGTACGTAGTTGAT
PRIMER_LEFT=165,22
PRIMER_RIGHT=438,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.919
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.1471
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.143555
PRIMER_LEFT_1_SEQUENCE=CACTAATGCAATGTGGGAAGAA
PRIMER_RIGHT_1_SEQUENCE=GACTCGAGGAAGCAGGAATAAA
PRIMER_LEFT_1=165,22
PRIMER_RIGHT_1=365,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.2137
PRIMER_LEFT_2_PENALTY=0.132857
PRIMER_RIGHT_2_PENALTY=0.080887
PRIMER_LEFT_2_SEQUENCE=GAAACACTAATGCAATGTGGGA
PRIMER_RIGHT_2_SEQUENCE=ATTGCCGGAGTACGTAGTTGAT
PRIMER_LEFT_2=161,22
PRIMER_RIGHT_2=438,22
PRIMER_LEFT_2_TM=59.867
PRIMER_RIGHT_2_TM=59.919
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.2764
PRIMER_LEFT_3_PENALTY=0.132857
PRIMER_RIGHT_3_PENALTY=0.143555
PRIMER_LEFT_3_SEQUENCE=GAAACACTAATGCAATGTGGGA
PRIMER_RIGHT_3_SEQUENCE=GACTCGAGGAAGCAGGAATAAA
PRIMER_LEFT_3=161,22
PRIMER_RIGHT_3=365,22
PRIMER_LEFT_3_TM=59.867
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=0.3279
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.324355
PRIMER_LEFT_4_SEQUENCE=CACTAATGCAATGTGGGAAGAA
PRIMER_RIGHT_4_SEQUENCE=TGTATGGGCATTAGAAAGAGGG
PRIMER_LEFT_4=165,22
PRIMER_RIGHT_4=398,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=60.324
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_03605 Ps_contig_mira-and-tgicl-ass_franssen_30535
SEQUENCE=CTTAGGTCGTTACCTTACCTATAGAACAATAGCAGTCTTTTATTCTTTCTATGTAAATTCATCATTATCACATCATCACAAACTTATGTTGCACATCAAATTCATGCATTTCATGTAGAGCTATTCTAAACCTACACGCATAATACTAACATCAAAGAACAGAAACACTAATGCAATGTGGGAAGAAAAAGAATAGGTCACACAATTGATACACATTAAGACCAACAACAGTAAGAGTAAACTAGAAACACATGAAACAACTTGGAATCTTAATCTACTAGCACCATTCTGCAGTTATCAGCAATAACATCTATGCCTACAGAGTACAAACAAACAAGCATCCTTTTATTCCTGCTTCCTCGAGTCTTTGAGCTGCCCCCTCTTTCTAATGCCCATACAAAACCCGAGGCGCCAAGTATCAACTACGTACTCCGGCAATGAACACGCCACAGCCCAAAGAAGGGTATGTGGCCAATAGGGTGTGCAACGAGGTTCGTATCCTATCCATTTCACACCTGCTTTCGCGTATCCGTCTGTTGATGGAACGAAGAATGAAGATCTCTTGATTGAAGCCATCTTTGTTGCCACATATAAAGGAACCTGACACTGCACATCAATCCCACTCTTCTTGTATTCAACATAGAGACATCTAGAGAATTGATCAATGTACGCTTTAGTGGCAGCATAAACCGCATAAAGTGGATCAGAAGGAATAACAATAGCAGCACCAGAACCAATGTTAACAATCGCTCCCTTCTTTCTCTTCAACATCCCAGGTAAAACAGCATGCGTAACCTTGGTAGTTCCCACAACATTCACCTTAATCAAATTCTTCAGAAGCTCCTCATCCAATTCATGGAAGAATCTCGCATAAGGGTAAGAAATCCCCACGTTGTTAACCAACACCCCAATATCCAACCCCTCAATCGCTTCACGAATTCTATCCACACCCTCGTCGAGATCACCGGTGAAATCAACGACAACGGTTTTAACCTCGGTTTTTCCGAACTTGGCTATGACAGAATCTGAAACGTCTTTGAGTTTGTCGGGGTTGCGACCGACAAGGATTAGGTTCAAGCCTTTACGAGCGAGTTCAAAGGCGAAGCTTTTTCCAATGCCGTCTGTGGGTCCGGTAACCAGTGCCCAAGATCCGTACTTTTTGAGGTTTTTGGGTTGTCTGAGAAAATTAACATAGACCCAGTTGAGGAATGTTAGAGTGAATCTGACAATGGTGATGAGACCGAGTGAGAAGAGAAGGAGGAACCAGAGAGGTTCGGATTTGAGTTTACTGATTATGCAGCAATCCATATTGAGGGTGGAAGGAAGAAGAAGAAGAAAATGGTAACAGTTGAGTTGTGAGGCGAAAAAGAGAGAGGGGTTTAATTATTTATATCAAGAANATAAGAGAGAGTAAGATT
TARGET=1323,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0597
PRIMER_LEFT_PENALTY=0.005674
PRIMER_RIGHT_PENALTY=0.054021
PRIMER_LEFT_SEQUENCE=CCCAAGATCCGTACTTTTTGAG
PRIMER_RIGHT_SEQUENCE=CTCTCTTTTTCGCCTCACAACT
PRIMER_LEFT=1142,22
PRIMER_RIGHT=1372,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.054
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.0664
PRIMER_LEFT_1_PENALTY=0.012411
PRIMER_RIGHT_1_PENALTY=0.054021
PRIMER_LEFT_1_SEQUENCE=TGAGAAGAGAAGGAGGAACCAG
PRIMER_RIGHT_1_SEQUENCE=CTCTCTTTTTCGCCTCACAACT
PRIMER_LEFT_1=1245,22
PRIMER_RIGHT_1=1372,22
PRIMER_LEFT_1_TM=59.988
PRIMER_RIGHT_1_TM=60.054
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=128
PRIMER_PAIR_PENALTY_2=0.1472
PRIMER_LEFT_2_PENALTY=0.093228
PRIMER_RIGHT_2_PENALTY=0.054021
PRIMER_LEFT_2_SEQUENCE=CGTACTTTTTGAGGTTTTTGGG
PRIMER_RIGHT_2_SEQUENCE=CTCTCTTTTTCGCCTCACAACT
PRIMER_LEFT_2=1151,22
PRIMER_RIGHT_2=1372,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=60.054
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.1472
PRIMER_LEFT_3_PENALTY=0.093228
PRIMER_RIGHT_3_PENALTY=0.054021
PRIMER_LEFT_3_SEQUENCE=CCGTACTTTTTGAGGTTTTTGG
PRIMER_RIGHT_3_SEQUENCE=CTCTCTTTTTCGCCTCACAACT
PRIMER_LEFT_3=1150,22
PRIMER_RIGHT_3=1372,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=60.054
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.1640
PRIMER_LEFT_4_PENALTY=0.005674
PRIMER_RIGHT_4_PENALTY=0.158317
PRIMER_LEFT_4_SEQUENCE=CCAAGATCCGTACTTTTTGAGG
PRIMER_RIGHT_4_SEQUENCE=CGCCTCACAACTCAACTGTTAC
PRIMER_LEFT_4=1143,22
PRIMER_RIGHT_4=1362,22
PRIMER_LEFT_4_TM=59.994
PRIMER_RIGHT_4_TM=59.842
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_03606 Ps_contig_mira-and-tgicl-ass_franssen_30538
SEQUENCE=CGGGGTGACCAACATTCTTTTCTTTTTCTCCTCTATGCACTTCTTAATTAGAAGAAACTACTAAAAAAACGTAGTAAAAACAAGTTCAATTACTATGCATAAACTCACACTCTTTCTCTTTTGTCTCATTTTCTCCGCACTCGCCGTTAGTTTTGCGGATCATGCAGACAAAACACAAAAAAATACCATTCACTCCAAAAGCATTTGTGATTCGCTATTGGGACAGAGTGATCAAAAACAACCTGCCAAAACCATCCTTCATCATTTCCAAGGCGTCGCCACTTAGCGCAGCAGACGCAACCGCTTTTGCCAAACACATAGCTGACAACACACTGTCTACAAAGTTGCCGGAATTCTGCTCAGCCGCACACCTCCTTTGCTTTCCTGATGTAAATCCTAGCCTTGCAAAACACTCTCAGGACTCCAAATTCACTGTCTACAACGAAGGTAACAACTTCACCAACTATGGAACAGAGAGACCAGGAGGACTAGATTCATTCAAGAACTACTCCAACGGTTTCGAAAGCAACCCGATCAACGAATTCCGCCAATACAGCCGCAGTTCCGCTGGCCACAACGACAGTTTCATCAACTATGCTGACTCAACCAACGTCGCAGATCAGAATTTCAACACCTACGGTTCTGCCGCCGCTGGTGGCGTCGGGGAATTCAAAGCATACGCTAAAGAAACCAACGTCCCTAACCTCAGGTTCTCCACTTACTCCGTCTCTACCGCCGGCAGACAACAGAGGTTCTCAAGCTACAGCGAAAACGGAAACGCCGGCGGTCAGGTTTTTGGTAACTACGGAAAAGACTCCGCAGGTGCCGAAAACCGTTTCACACAATACGGAACGGACTCTAACGTTGCTTCATCAGGTTTTTCTAGTTACGCGGACAAAGGCACAGGTAATAAAGACACTTTCGTTAGTTACGGCTTTAATATGAATAATCCTACTGTGGATTTCAAAAACTACGCCAGAGGAGGTTTAGGAGTCACTGAGAAGTTCTCAAGTTATAGAGATCAATCCAATGTTGGTGCTGATTCGTTTACTTCTTACGCTAAGGATGCAGAAACTGGGACCCATGTTGATTTCAATAACTATGGAAAATCTTTTAACGAAGGAACCGACACTTTCAAAGGCTATGCTAAAGGTGCAGGTGCAGATCATAAAGTTACTTTCAAAAGTTATGGTGTGAATAACACTTTCAAGGATTACGATAAAAAAGGTATTTCTTTTGCCCAATATACTAACGCTTCTGCAACTAATGCTGTTTCTGTTAGTAATAGTTTGGCAAAGAAATGGGTTGAACCGGGTAAGTTTTTCCGGGAAATGATGTTGAAACAAGGTGTGGTAATGCCTATGCCTGATATCAAAGATAAGTTACCAAAAAGATCGTTTTTGCCTCGTAACATTTTCACTAAATTGCCCTTTGCAACTTCGAAGATGAATGAGTTGAAGCAAGTTTTTAAAGTGTCTGAGAATTCGTCAATGGATAAAATGATAGTGGACTCGTTGGGTGAATGTGAGAGGGCTCCGAGTAAGGGCGAGACGAAACGGTGTGTAGGATCACTCGAGGACATGATCGACTTTGCAACTTCGGTATTGGGCCATGATGTTACGGTTCGGTCCACGGAGAATTTAAATGGGTCGGGTAAAAATGTTATGGTGGGTCGGGTTCAAGGGATAAACCATGGAAAAGTGACTAAATCAGTTTCATGTCATCAGAGTTTGTTTCCTTCTTTGTTGTACTATTGTCACTCAGTTCCGAAGGTTCGGGTTTATGAGGCGGATCTTTTGGACCCGGAATCAAAGGCCAAGATTAATCACGGTGTTGCGATTTGTCATTTGGATACAACTGCTTGGAGCCCCACCCATGGTGCATTCATGGCTCTTGGGTCTGGTCCGGGTCGAATTGAAGTGTGCCATTGGATCTTTGAGAACGATATGACTTGGACTACTACGGACTAAAGAGAACTCCAACCAAAATATATATATGCTTTTATTTCAGTGTAATGTTATGTCAGCAATAACGAGTTTATATTTTCCTATTTTTAGAAATAAATGATTATGTTATTGGAAGAAAATGTACTATTTATTACCTATGTATGTTTGGATTGAAGATCAGTTTGAGATAATTATAATGTGNCAATGTGATTTAAGAGC
TARGET=1988,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03607 Ps_contig_mira-and-tgicl-ass_franssen_30540
SEQUENCE=AGTATAAACGCAAATCATNATTTAAAAAAGGAATTTGGAAACATGCTTCTTCAAAATAAAACTGCAGGTAACATCATCCAGCCATAAAATTCTGGAAATGCAAGATCAACATGTCACATTGCGCAACATAAATTAAACAAGATCCTCGAAGTACCATCGACTCTACAGGCTAAAACGAAGAACCAATTACTGCAACGGGAAACAAAGATACCATAGTGCAAAACACGAGGACAAAAGTTTCAACTAAACGCCACAGGTTCTTTCGAACATATATGTTTCTATGTACGTATATATATGTAGCAGCAGCAGTCAAACTCCAGCAGCAGCAATGACAATCGGTTCAGTTCATGGACTACGAGGAGAACGAGCACGGACTGGAGTCTTTGACGGACTCACCCTTATAGGCAGAGCACCCAAAATCACGCCACTGCAGCTTAGGGCGGCAATTGCACTGTCAGCCACTGTGAACTCGACAAATGCAATCCGAGTTGAATGATGGTAATCCCCAAGAAGCCTCAGTCGTTGAACCTCTCCACAGATAGATTCAAAGAAGTGTTTCACATCTGCTTGAGTCAGCTTTTTGTCGATGTTTGTGCAGTAAATTGTTCTAGAGCACATCTCCCTTTCATCTTCAGACCTGGGCAAAAATGTCGGATTGACAGGTGCAATGGCAGTTTTGGAAGGCAGCACTCTCAACGGGTAATATCCCAGCATAGTTCCAGAAAGGCTTACAGCAGCTCTTGCACTTTCTTCATCGGTAAACTCAATGAAGGCAAAACGGAGAATAGAGTTGGGATCTCCGCAAACACGGCAGTCAACAACCTGGCCGCAATTAAGAAAGAGAGATGCCAGCTGCTCTTCAGTTACCTGTTGATCAATGTCAGACACATAAACGGTCCTCCTAATCATCTCTTCTCGTTTCTCCATATCTACTTTATGATTCACTCTTCGCTTTCCATTAGTACTATAGCCAGTCTTCCTCCTTCGGTTAGTTTGGCCGTTAGCATTAGCATAGTTATTGAGAAGTATATAATTGTTAGGGTAACCAAACCCAGCAGCAGCTCCAGGTAAGTATCCGTGATTATTGGTTAGCGATGGTGGCACAAATTCCTCGGCCATGGGATTCAGCTTCGACAACAATTCCTCCAAATCCCTCATTTCACGCTTGAAACTCTCGCCCTCGTTCTCACCGTTCATCACATACCCATCATTGTTCATCACAAACTGCTGATTCTGAGCCCCGTTAGCATGCATCTGACCCAATTGATGAGCCTTGAAGCTATAATTACCACCATTCGGAAGTTGATGGTTGAACACAGCGTTGTTGTTGATGGTCACATCCTGATCGCTCCTAGGTTTCGATTTCTCCACCTCAGTTGAGTCAGATGATACAACGTTGTTGGTGTTGTTGTTGTCCAAATTGGCACTAACATTATCAGCAACAGCCATAATCACTGGTGTTGAGGTAAAAAGTTTCTACCTTTATTCTCCGATATCAGATTTTCCTTCGAATCAGTATTAAATTACTGATGAAGATTAAGAAGAAGAAGAAGAAGATTGTTGAGATGATGATGATTGAGAAGAGAGATGAAAAGGGTATTGTTCGGATCGTCGGTTTTCGCAGCACTGACTTTTTGGACCTTCCTGAGAAGAACGTCCCAATTTGATGTCTTTGAGTTGTGAATGTGACCATG
TARGET=1404,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2933
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=0.183834
PRIMER_LEFT_SEQUENCE=TTTCGATTTCTCCACCTCAGTT
PRIMER_RIGHT_SEQUENCE=TCCGAACAATACCCTTTTCATC
PRIMER_LEFT=1356,22
PRIMER_RIGHT=1607,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=60.184
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.2933
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.183834
PRIMER_LEFT_1_SEQUENCE=TTTCGATTTCTCCACCTCAGTT
PRIMER_RIGHT_1_SEQUENCE=GATCCGAACAATACCCTTTTCA
PRIMER_LEFT_1=1356,22
PRIMER_RIGHT_1=1609,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.184
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=254
PRIMER_PAIR_PENALTY_2=0.3147
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.205226
PRIMER_LEFT_2_SEQUENCE=TTTCGATTTCTCCACCTCAGTT
PRIMER_RIGHT_2_SEQUENCE=GAAGGTCCAAAAAGTCAGTGCT
PRIMER_LEFT_2=1356,22
PRIMER_RIGHT_2=1643,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.795
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.3231
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.213575
PRIMER_LEFT_3_SEQUENCE=TTTCGATTTCTCCACCTCAGTT
PRIMER_RIGHT_3_SEQUENCE=TTCTTCTCAGGAAGGTCCAAAA
PRIMER_LEFT_3=1356,22
PRIMER_RIGHT_3=1653,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=60.214
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.3231
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.213575
PRIMER_LEFT_4_SEQUENCE=TTTCGATTTCTCCACCTCAGTT
PRIMER_RIGHT_4_SEQUENCE=TCTTCTCAGGAAGGTCCAAAAA
PRIMER_LEFT_4=1356,22
PRIMER_RIGHT_4=1652,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=60.214
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_03608 Ps_contig_mira-and-tgicl-ass_franssen_30540
SEQUENCE=AGTATAAACGCAAATCATNATTTAAAAAAGGAATTTGGAAACATGCTTCTTCAAAATAAAACTGCAGGTAACATCATCCAGCCATAAAATTCTGGAAATGCAAGATCAACATGTCACATTGCGCAACATAAATTAAACAAGATCCTCGAAGTACCATCGACTCTACAGGCTAAAACGAAGAACCAATTACTGCAACGGGAAACAAAGATACCATAGTGCAAAACACGAGGACAAAAGTTTCAACTAAACGCCACAGGTTCTTTCGAACATATATGTTTCTATGTACGTATATATATGTAGCAGCAGCAGTCAAACTCCAGCAGCAGCAATGACAATCGGTTCAGTTCATGGACTACGAGGAGAACGAGCACGGACTGGAGTCTTTGACGGACTCACCCTTATAGGCAGAGCACCCAAAATCACGCCACTGCAGCTTAGGGCGGCAATTGCACTGTCAGCCACTGTGAACTCGACAAATGCAATCCGAGTTGAATGATGGTAATCCCCAAGAAGCCTCAGTCGTTGAACCTCTCCACAGATAGATTCAAAGAAGTGTTTCACATCTGCTTGAGTCAGCTTTTTGTCGATGTTTGTGCAGTAAATTGTTCTAGAGCACATCTCCCTTTCATCTTCAGACCTGGGCAAAAATGTCGGATTGACAGGTGCAATGGCAGTTTTGGAAGGCAGCACTCTCAACGGGTAATATCCCAGCATAGTTCCAGAAAGGCTTACAGCAGCTCTTGCACTTTCTTCATCGGTAAACTCAATGAAGGCAAAACGGAGAATAGAGTTGGGATCTCCGCAAACACGGCAGTCAACAACCTGGCCGCAATTAAGAAAGAGAGATGCCAGCTGCTCTTCAGTTACCTGTTGATCAATGTCAGACACATAAACGGTCCTCCTAATCATCTCTTCTCGTTTCTCCATATCTACTTTATGATTCACTCTTCGCTTTCCATTAGTACTATAGCCAGTCTTCCTCCTTCGGTTAGTTTGGCCGTTAGCATTAGCATAGTTATTGAGAAGTATATAATTGTTAGGGTAACCAAACCCAGCAGCAGCTCCAGGTAAGTATCCGTGATTATTGGTTAGCGATGGTGGCACAAATTCCTCGGCCATGGGATTCAGCTTCGACAACAATTCCTCCAAATCCCTCATTTCACGCTTGAAACTCTCGCCCTCGTTCTCACCGTTCATCACATACCCATCATTGTTCATCACAAACTGCTGATTCTGAGCCCCGTTAGCATGCATCTGACCCAATTGATGAGCCTTGAAGCTATAATTACCACCATTCGGAAGTTGATGGTTGAACACAGCGTTGTTGTTGATGGTCACATCCTGATCGCTCCTAGGTTTCGATTTCTCCACCTCAGTTGAGTCAGATGATACAACGTTGTTGGTGTTGTTGTTGTCCAAATTGGCACTAACATTATCAGCAACAGCCATAATCACTGGTGTTGAGGTAAAAAGTTTCTACCTTTATTCTCCGATATCAGATTTTCCTTCGAATCAGTATTAAATTACTGATGAAGATTAAGAAGAAGAAGAAGAAGATTGTTGAGATGATGATGATTGAGAAGAGAGATGAAAAGGGTATTGTTCGGATCGTCGGTTTTCGCAGCACTGACTTTTTGGACCTTCCTGAGAAGAACGTCCCAATTTGATGTCTTTGAGTTGTGAATGTGACCATG
TARGET=1539,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2635
PRIMER_LEFT_PENALTY=0.079714
PRIMER_RIGHT_PENALTY=0.183834
PRIMER_LEFT_SEQUENCE=ACAGCCATAATCACTGGTGTTG
PRIMER_RIGHT_SEQUENCE=TCCGAACAATACCCTTTTCATC
PRIMER_LEFT=1441,22
PRIMER_RIGHT=1607,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.184
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.2635
PRIMER_LEFT_1_PENALTY=0.079714
PRIMER_RIGHT_1_PENALTY=0.183834
PRIMER_LEFT_1_SEQUENCE=ACAGCCATAATCACTGGTGTTG
PRIMER_RIGHT_1_SEQUENCE=GATCCGAACAATACCCTTTTCA
PRIMER_LEFT_1=1441,22
PRIMER_RIGHT_1=1609,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=60.184
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.2849
PRIMER_LEFT_2_PENALTY=0.079714
PRIMER_RIGHT_2_PENALTY=0.205226
PRIMER_LEFT_2_SEQUENCE=ACAGCCATAATCACTGGTGTTG
PRIMER_RIGHT_2_SEQUENCE=GAAGGTCCAAAAAGTCAGTGCT
PRIMER_LEFT_2=1441,22
PRIMER_RIGHT_2=1643,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=59.795
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.2933
PRIMER_LEFT_3_PENALTY=0.079714
PRIMER_RIGHT_3_PENALTY=0.213575
PRIMER_LEFT_3_SEQUENCE=ACAGCCATAATCACTGGTGTTG
PRIMER_RIGHT_3_SEQUENCE=TCTTCTCAGGAAGGTCCAAAAA
PRIMER_LEFT_3=1441,22
PRIMER_RIGHT_3=1652,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=60.214
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.2933
PRIMER_LEFT_4_PENALTY=0.079714
PRIMER_RIGHT_4_PENALTY=0.213575
PRIMER_LEFT_4_SEQUENCE=ACAGCCATAATCACTGGTGTTG
PRIMER_RIGHT_4_SEQUENCE=TTCTTCTCAGGAAGGTCCAAAA
PRIMER_LEFT_4=1441,22
PRIMER_RIGHT_4=1653,22
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=60.214
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_03609 Ps_contig_mira-and-tgicl-ass_franssen_30540
SEQUENCE=AGTATAAACGCAAATCATNATTTAAAAAAGGAATTTGGAAACATGCTTCTTCAAAATAAAACTGCAGGTAACATCATCCAGCCATAAAATTCTGGAAATGCAAGATCAACATGTCACATTGCGCAACATAAATTAAACAAGATCCTCGAAGTACCATCGACTCTACAGGCTAAAACGAAGAACCAATTACTGCAACGGGAAACAAAGATACCATAGTGCAAAACACGAGGACAAAAGTTTCAACTAAACGCCACAGGTTCTTTCGAACATATATGTTTCTATGTACGTATATATATGTAGCAGCAGCAGTCAAACTCCAGCAGCAGCAATGACAATCGGTTCAGTTCATGGACTACGAGGAGAACGAGCACGGACTGGAGTCTTTGACGGACTCACCCTTATAGGCAGAGCACCCAAAATCACGCCACTGCAGCTTAGGGCGGCAATTGCACTGTCAGCCACTGTGAACTCGACAAATGCAATCCGAGTTGAATGATGGTAATCCCCAAGAAGCCTCAGTCGTTGAACCTCTCCACAGATAGATTCAAAGAAGTGTTTCACATCTGCTTGAGTCAGCTTTTTGTCGATGTTTGTGCAGTAAATTGTTCTAGAGCACATCTCCCTTTCATCTTCAGACCTGGGCAAAAATGTCGGATTGACAGGTGCAATGGCAGTTTTGGAAGGCAGCACTCTCAACGGGTAATATCCCAGCATAGTTCCAGAAAGGCTTACAGCAGCTCTTGCACTTTCTTCATCGGTAAACTCAATGAAGGCAAAACGGAGAATAGAGTTGGGATCTCCGCAAACACGGCAGTCAACAACCTGGCCGCAATTAAGAAAGAGAGATGCCAGCTGCTCTTCAGTTACCTGTTGATCAATGTCAGACACATAAACGGTCCTCCTAATCATCTCTTCTCGTTTCTCCATATCTACTTTATGATTCACTCTTCGCTTTCCATTAGTACTATAGCCAGTCTTCCTCCTTCGGTTAGTTTGGCCGTTAGCATTAGCATAGTTATTGAGAAGTATATAATTGTTAGGGTAACCAAACCCAGCAGCAGCTCCAGGTAAGTATCCGTGATTATTGGTTAGCGATGGTGGCACAAATTCCTCGGCCATGGGATTCAGCTTCGACAACAATTCCTCCAAATCCCTCATTTCACGCTTGAAACTCTCGCCCTCGTTCTCACCGTTCATCACATACCCATCATTGTTCATCACAAACTGCTGATTCTGAGCCCCGTTAGCATGCATCTGACCCAATTGATGAGCCTTGAAGCTATAATTACCACCATTCGGAAGTTGATGGTTGAACACAGCGTTGTTGTTGATGGTCACATCCTGATCGCTCCTAGGTTTCGATTTCTCCACCTCAGTTGAGTCAGATGATACAACGTTGTTGGTGTTGTTGTTGTCCAAATTGGCACTAACATTATCAGCAACAGCCATAATCACTGGTGTTGAGGTAAAAAGTTTCTACCTTTATTCTCCGATATCAGATTTTCCTTCGAATCAGTATTAAATTACTGATGAAGATTAAGAAGAAGAAGAAGAAGATTGTTGAGATGATGATGATTGAGAAGAGAGATGAAAAGGGTATTGTTCGGATCGTCGGTTTTCGCAGCACTGACTTTTTGGACCTTCCTGAGAAGAACGTCCCAATTTGATGTCTTTGAGTTGTGAATGTGACCATG
TARGET=1565,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2635
PRIMER_LEFT_PENALTY=0.079714
PRIMER_RIGHT_PENALTY=0.183834
PRIMER_LEFT_SEQUENCE=ACAGCCATAATCACTGGTGTTG
PRIMER_RIGHT_SEQUENCE=TCCGAACAATACCCTTTTCATC
PRIMER_LEFT=1441,22
PRIMER_RIGHT=1607,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.184
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.2635
PRIMER_LEFT_1_PENALTY=0.079714
PRIMER_RIGHT_1_PENALTY=0.183834
PRIMER_LEFT_1_SEQUENCE=ACAGCCATAATCACTGGTGTTG
PRIMER_RIGHT_1_SEQUENCE=GATCCGAACAATACCCTTTTCA
PRIMER_LEFT_1=1441,22
PRIMER_RIGHT_1=1609,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=60.184
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.2849
PRIMER_LEFT_2_PENALTY=0.079714
PRIMER_RIGHT_2_PENALTY=0.205226
PRIMER_LEFT_2_SEQUENCE=ACAGCCATAATCACTGGTGTTG
PRIMER_RIGHT_2_SEQUENCE=GAAGGTCCAAAAAGTCAGTGCT
PRIMER_LEFT_2=1441,22
PRIMER_RIGHT_2=1643,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=59.795
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.2933
PRIMER_LEFT_3_PENALTY=0.079714
PRIMER_RIGHT_3_PENALTY=0.213575
PRIMER_LEFT_3_SEQUENCE=ACAGCCATAATCACTGGTGTTG
PRIMER_RIGHT_3_SEQUENCE=TCTTCTCAGGAAGGTCCAAAAA
PRIMER_LEFT_3=1441,22
PRIMER_RIGHT_3=1652,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=60.214
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.2933
PRIMER_LEFT_4_PENALTY=0.079714
PRIMER_RIGHT_4_PENALTY=0.213575
PRIMER_LEFT_4_SEQUENCE=ACAGCCATAATCACTGGTGTTG
PRIMER_RIGHT_4_SEQUENCE=TTCTTCTCAGGAAGGTCCAAAA
PRIMER_LEFT_4=1441,22
PRIMER_RIGHT_4=1653,22
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=60.214
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_03610 Ps_contig_mira-and-tgicl-ass_franssen_30541
SEQUENCE=CTTCTCCTCCTTTTCCTATCTCTATATCTCCATTTCTCCAATTTTCTTCCTCCCCTTTATTGTACCTTTGGTCACCACTCTCAAATACCCATTTCTCTATTTTCTGGTTTAAAGGTCTGAAAAAAGGTTGATAACTCTTTGANTTGAGTTGGTGTTTGAAGCTAAACAAAGGTTTTTGAAACCCAATTTACAGGTTGAAGCTGATTTTTCTGGAAATTTGGGCCATCTTTAGCGGTGTTTTCCTGAGAAAAGATGATGATGTTTGATGACATGGGGTTTTGTGGTGATTTGGATATGTTCTGTGGTACACTTGGGGAAGGGGATATTTCTGTGAGGCAAACCGAGCCTGATTCGGTGGTTGAAGATGATTACAGTGATGAAGAAATGGATGTAGACGAGCTTGAGCGTAGGATGTGGAGGGACAAAATGCGTCTGAAGCGTTTGAAAGAACAAACCAAGGCTAAAGAAGGGATCGATGCTGCAAAGGCTAGGCAGTCTCAAGAACAGGCAAGGAGGAAGAAGATGTCGAGAGCTCAAGATGGAATACTGAAGTACATGCTGAAAATGATGGAGGTTTGTAAGGCACAAGGATTCGTTTACGGGATAATTCCTGAGAAGGGAAAACCAGTGACCGGAGCATCTGACAATCTTCGTGAATGGTGGAAGGATAAGGTTAGGTTCGATAGGAACGGTCCGGCTGCCATAGCCAAATATCAAGTGGATAACGCAATTCCAGGAAACAGTGACGGCTGCAACTCCATCGGTCCAACTCCTCATACCTTACAAGAACTACAGGATACAACGTTAGGTTCTCTCTTATCGGCACTTATGCAGCACTGTGATCCTCCTCAGAGGCGTTTCCCCTTAGAGAAGGGTAACCCTCCGCCATGGTGGCCAACCGGGAACGAAGTGTGGTGGCCCCAAATTGGTTTACCTAAAGATCAAGGCCCTCCACCTTACAAGAAACCTCATGACTTGAAAAAAGCATGGAAGGTTGGTGTTTTAACTGCAGTTATCAAGCATATGTCTCCTGATATAGCCAAGATTCGCAAGCTCGTGAGGCAGTCCAAATGCCTTCAAGATAAAATGACGGCCAAAGAAAGTGCTACCTGGCTGGCCATCATCAATCAAGAGGAGTCCTTAGCTCGAGAGCTTTTTCCTGAGTATTGTCCTCCGATGTCTTCTGGTGGAGGCAGCGGTTCTATGGTCATCAACGATTGCAGCGAGTATGATGTTGATGGGGCTGAGGATGAGTCCAACTTTGACGTGGAGGACCGGAAACCCGAGAATCTTCATCCATCCAATCTTGGGATGGAGAGAATGAGGGGAATGTTTCCGGTTCAGCAACTTTCTTCACCAATCAAAGGAGAGGTTGTCACCAACTTGGATTTCATTCGGAAGAGGAAGATCTCTAGCGACTTCAACATAATGATGGATCACAAAATCTACACTTGTGAACACCCTCAATGCCCTTACAACGAAGTTCGCCTTGCTTTTCAAGACAGGCCTTCTAGGGACAATCATCAATTGAATTGCCCACATAGAATCGGTTATGGCGGCCCTAATTTTCACGCTACTGAGGTTAAGCCCGTTATTTTCCCACAAAGCTTTGTTCAACCCAATTCTGCAGCTCAGCCTACTAGTCTGGTTCCACCTTCATTTGATTTAACCGGACTCGGGGTTTCAGAGGACAGCCAGAAAATGATCAGCGACCTCATGTCAGTCTACGACACAAACATCACAGGAAACAAGAATGCAAGTTCAACCAATTGCATAGCTGTCGGAAATCAAAACCTTTCTCAGCACAACATTCAGCAACAACAACAGGAAAATTTCTTTTCAGGTCAAGGAATGGACATCATAGGAAACAAGACTGCAAGTTCAACCAATTGCGTAGCTGTCGGAAATCAAACCCTTTCTCAGCACAACATTCAGCAACAACAACAGGAGAATTTCTTTTCAAGTCAAGGAATGGTGATGGAATCAAACTTCTTTCCTCGCGACGAAAATCAATTCGACCGATTCAAAGCCGTAAACACCACTCCTTTCGAAACCAACCACCACCACAACAACAACACCACCAATAACACCCATTTCATGATTGGCTCTCCATGTGATCTGGCATCCTTTGATTTTAGGGAGGACATTCAACAACTACAAGGAGTAGTAGGAATGGATAATCTTCACAAACAACCAGATGGTTCAATATGGGGCTACAACTGAAAGTAAAACTTGTGTTCCAATGGCTTTTTTATTGTGTCATTAAAAAGGGATATAGACACACAAACACACACTCTTATCATGTGAAATCCCTTGACTCAAAAATCAAAGGTAGTTTAGTTTACTGCATTTTTCTTCACATGTAATCGTTTAGGTCTAGTCAGGTCTTGTTCTGGTATGTGGCAATAAATCATGTTGATGTTATGTTTCCATAATGTAACCAACCAACCAAACCCTCTTGTGTTTTCATCACTTTCACCTCTATTATATTGTTGTTATGTACTACTTCTGTGATATAAATAAGATTTTTGTACTTTATTCCACCANACTTTTTTTCTTGGTGCTACTGAGACATACCTTAA
TARGET=2066,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0487
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.025128
PRIMER_LEFT_SEQUENCE=CTTTTCAGGTCAAGGAATGGAC
PRIMER_RIGHT_SEQUENCE=CATGAAATGGGTGTTATTGGTG
PRIMER_LEFT=1835,22
PRIMER_RIGHT=2099,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.975
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.0544
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.025128
PRIMER_LEFT_1_SEQUENCE=TTCAAGTCAAGGAATGGTGATG
PRIMER_RIGHT_1_SEQUENCE=CATGAAATGGGTGTTATTGGTG
PRIMER_LEFT_1=1958,22
PRIMER_RIGHT_1=2099,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.975
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=142
PRIMER_PAIR_PENALTY_2=0.0650
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.025128
PRIMER_LEFT_2_SEQUENCE=AACCCTTTCTCAGCACAACATT
PRIMER_RIGHT_2_SEQUENCE=CATGAAATGGGTGTTATTGGTG
PRIMER_LEFT_2=1910,22
PRIMER_RIGHT_2=2099,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.0650
PRIMER_LEFT_3_PENALTY=0.039879
PRIMER_RIGHT_3_PENALTY=0.025128
PRIMER_LEFT_3_SEQUENCE=AAACCCTTTCTCAGCACAACAT
PRIMER_RIGHT_3_SEQUENCE=CATGAAATGGGTGTTATTGGTG
PRIMER_LEFT_3=1909,22
PRIMER_RIGHT_3=2099,22
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=59.975
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=0.0844
PRIMER_LEFT_4_PENALTY=0.059316
PRIMER_RIGHT_4_PENALTY=0.025128
PRIMER_LEFT_4_SEQUENCE=CTTTCTCAGCACAACATTCAGC
PRIMER_RIGHT_4_SEQUENCE=CATGAAATGGGTGTTATTGGTG
PRIMER_LEFT_4=1914,22
PRIMER_RIGHT_4=2099,22
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=59.975
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_03611 Ps_contig_mira-and-tgicl-ass_franssen_30541
SEQUENCE=CTTCTCCTCCTTTTCCTATCTCTATATCTCCATTTCTCCAATTTTCTTCCTCCCCTTTATTGTACCTTTGGTCACCACTCTCAAATACCCATTTCTCTATTTTCTGGTTTAAAGGTCTGAAAAAAGGTTGATAACTCTTTGANTTGAGTTGGTGTTTGAAGCTAAACAAAGGTTTTTGAAACCCAATTTACAGGTTGAAGCTGATTTTTCTGGAAATTTGGGCCATCTTTAGCGGTGTTTTCCTGAGAAAAGATGATGATGTTTGATGACATGGGGTTTTGTGGTGATTTGGATATGTTCTGTGGTACACTTGGGGAAGGGGATATTTCTGTGAGGCAAACCGAGCCTGATTCGGTGGTTGAAGATGATTACAGTGATGAAGAAATGGATGTAGACGAGCTTGAGCGTAGGATGTGGAGGGACAAAATGCGTCTGAAGCGTTTGAAAGAACAAACCAAGGCTAAAGAAGGGATCGATGCTGCAAAGGCTAGGCAGTCTCAAGAACAGGCAAGGAGGAAGAAGATGTCGAGAGCTCAAGATGGAATACTGAAGTACATGCTGAAAATGATGGAGGTTTGTAAGGCACAAGGATTCGTTTACGGGATAATTCCTGAGAAGGGAAAACCAGTGACCGGAGCATCTGACAATCTTCGTGAATGGTGGAAGGATAAGGTTAGGTTCGATAGGAACGGTCCGGCTGCCATAGCCAAATATCAAGTGGATAACGCAATTCCAGGAAACAGTGACGGCTGCAACTCCATCGGTCCAACTCCTCATACCTTACAAGAACTACAGGATACAACGTTAGGTTCTCTCTTATCGGCACTTATGCAGCACTGTGATCCTCCTCAGAGGCGTTTCCCCTTAGAGAAGGGTAACCCTCCGCCATGGTGGCCAACCGGGAACGAAGTGTGGTGGCCCCAAATTGGTTTACCTAAAGATCAAGGCCCTCCACCTTACAAGAAACCTCATGACTTGAAAAAAGCATGGAAGGTTGGTGTTTTAACTGCAGTTATCAAGCATATGTCTCCTGATATAGCCAAGATTCGCAAGCTCGTGAGGCAGTCCAAATGCCTTCAAGATAAAATGACGGCCAAAGAAAGTGCTACCTGGCTGGCCATCATCAATCAAGAGGAGTCCTTAGCTCGAGAGCTTTTTCCTGAGTATTGTCCTCCGATGTCTTCTGGTGGAGGCAGCGGTTCTATGGTCATCAACGATTGCAGCGAGTATGATGTTGATGGGGCTGAGGATGAGTCCAACTTTGACGTGGAGGACCGGAAACCCGAGAATCTTCATCCATCCAATCTTGGGATGGAGAGAATGAGGGGAATGTTTCCGGTTCAGCAACTTTCTTCACCAATCAAAGGAGAGGTTGTCACCAACTTGGATTTCATTCGGAAGAGGAAGATCTCTAGCGACTTCAACATAATGATGGATCACAAAATCTACACTTGTGAACACCCTCAATGCCCTTACAACGAAGTTCGCCTTGCTTTTCAAGACAGGCCTTCTAGGGACAATCATCAATTGAATTGCCCACATAGAATCGGTTATGGCGGCCCTAATTTTCACGCTACTGAGGTTAAGCCCGTTATTTTCCCACAAAGCTTTGTTCAACCCAATTCTGCAGCTCAGCCTACTAGTCTGGTTCCACCTTCATTTGATTTAACCGGACTCGGGGTTTCAGAGGACAGCCAGAAAATGATCAGCGACCTCATGTCAGTCTACGACACAAACATCACAGGAAACAAGAATGCAAGTTCAACCAATTGCATAGCTGTCGGAAATCAAAACCTTTCTCAGCACAACATTCAGCAACAACAACAGGAAAATTTCTTTTCAGGTCAAGGAATGGACATCATAGGAAACAAGACTGCAAGTTCAACCAATTGCGTAGCTGTCGGAAATCAAACCCTTTCTCAGCACAACATTCAGCAACAACAACAGGAGAATTTCTTTTCAAGTCAAGGAATGGTGATGGAATCAAACTTCTTTCCTCGCGACGAAAATCAATTCGACCGATTCAAAGCCGTAAACACCACTCCTTTCGAAACCAACCACCACCACAACAACAACACCACCAATAACACCCATTTCATGATTGGCTCTCCATGTGATCTGGCATCCTTTGATTTTAGGGAGGACATTCAACAACTACAAGGAGTAGTAGGAATGGATAATCTTCACAAACAACCAGATGGTTCAATATGGGGCTACAACTGAAAGTAAAACTTGTGTTCCAATGGCTTTTTTATTGTGTCATTAAAAAGGGATATAGACACACAAACACACACTCTTATCATGTGAAATCCCTTGACTCAAAAATCAAAGGTAGTTTAGTTTACTGCATTTTTCTTCACATGTAATCGTTTAGGTCTAGTCAGGTCTTGTTCTGGTATGTGGCAATAAATCATGTTGATGTTATGTTTCCATAATGTAACCAACCAACCAAACCCTCTTGTGTTTTCATCACTTTCACCTCTATTATATTGTTGTTATGTACTACTTCTGTGATATAAATAAGATTTTTGTACTTTATTCCACCANACTTTTTTTCTTGGTGCTACTGAGACATACCTTAA
TARGET=2439,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.2130
PRIMER_LEFT_PENALTY=0.439553
PRIMER_RIGHT_PENALTY=1.773411
PRIMER_LEFT_SEQUENCE=ATGGTTCAATATGGGGCTACAA
PRIMER_RIGHT_SEQUENCE=AAGTGATGAAAACACAAGAGGG
PRIMER_LEFT=2197,22
PRIMER_RIGHT=2474,22
PRIMER_LEFT_TM=60.440
PRIMER_RIGHT_TM=58.227
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=278
PRIMER_PAIR_PENALTY_1=2.2995
PRIMER_LEFT_1_PENALTY=0.526059
PRIMER_RIGHT_1_PENALTY=1.773411
PRIMER_LEFT_1_SEQUENCE=TCAATATGGGGCTACAACTGAA
PRIMER_RIGHT_1_SEQUENCE=AAGTGATGAAAACACAAGAGGG
PRIMER_LEFT_1=2202,22
PRIMER_RIGHT_1=2474,22
PRIMER_LEFT_1_TM=59.474
PRIMER_RIGHT_1_TM=58.227
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=2.3206
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=2.283237
PRIMER_LEFT_2_SEQUENCE=GTCTTGTTCTGGTATGTGGCAA
PRIMER_RIGHT_2_SEQUENCE=GGTATGTCTCAGTAGCACCAAGAA
PRIMER_LEFT_2=2384,22
PRIMER_RIGHT_2=2577,24
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=59.717
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.833
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=2.3575
PRIMER_LEFT_3_PENALTY=0.439553
PRIMER_RIGHT_3_PENALTY=1.917925
PRIMER_LEFT_3_SEQUENCE=ATGGTTCAATATGGGGCTACAA
PRIMER_RIGHT_3_SEQUENCE=GTGATGAAAACACAAGAGGGTT
PRIMER_LEFT_3=2197,22
PRIMER_RIGHT_3=2472,22
PRIMER_LEFT_3_TM=60.440
PRIMER_RIGHT_3_TM=58.082
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=2.4440
PRIMER_LEFT_4_PENALTY=0.526059
PRIMER_RIGHT_4_PENALTY=1.917925
PRIMER_LEFT_4_SEQUENCE=TCAATATGGGGCTACAACTGAA
PRIMER_RIGHT_4_SEQUENCE=GTGATGAAAACACAAGAGGGTT
PRIMER_LEFT_4=2202,22
PRIMER_RIGHT_4=2472,22
PRIMER_LEFT_4_TM=59.474
PRIMER_RIGHT_4_TM=58.082
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_03612 Ps_contig_mira-and-tgicl-ass_franssen_30543
SEQUENCE=ACTTTTACTAGAGATTTTTCCTTGTAAACAAAAAACAAGGACATGAAAACCAAAAATAATTTCAAATGAAAAAATAAAATAAACGAGCCTTATCAGAAATGTCCTCTTTATATTTTTAATTTTTAATTTTTAATTCAGGATTCAAAAAAGAAATATAAAAAAAAAGTGAACAAAATTTTGTTCTACGGTTTCTCCTTGAGCAGAGAGGCTAATCCGGTCACAACCTTCGTTCCTCTTTCAACACTCTTCGGTTTCACCGCCGGTAACTGCCACGCTGGACTCACCGTAACTACCTCTTCCGGCGTTGCTTTCACTTCACCGAAAATCAGCTCCAATTCTCCAGAATCGTTATTCACATCTTGCGTCGGTGTTTCTTGAGCCGCCGCATCGGCGACTTGCTTTGACCTAGCAGCAGAGGGAGAGATCCATTTGGCAAGCATGTAATCGCGCTTCCTCCACGGTGGAACATGCATCCCTTTCTTCTTCAAACTCTGTTTCGCCGCCTCCGATACGATGGACACGATCTGCTTCAGTCGTTCCTTTTTACCAACGAAGATGTACGGTAGCGATTGAAGGATTGTCTTGTAGGTTCCTGTAGATCGCGCGATCTCAAATTCCGATCGAAAATCGATGTCGATTATCAGTGTTTCACCTTCCACAATCGCATCAATGAATTGATATTCACCAGCAGGACAGGAAGAAGTTTTCTCCCATTTCGATTTGCAAATTGAAGAATCGTATCCCAGAGAGGAAAGTGCTTCTGAAACTATCTTAATCAAATCTTCTTTCCTTTTGTAAACCTTGCTATTCTTCTCAACAATCTTCGCCGCATCGGCTAGTAAATTCCTCTCTTCAACAGTTACACAAGGAATCAAGCTCTTGAGCGAGTCATTGGAATCGGCGACTGATTCATGTGTAAAACCGGAGAGATCGAAATCGTCCTCGTCGGAGCTACTCGCGTTAAAGCAGTTACAGCGGTTACGACCGCATCTAAGAGGAGGTGGTTGAGGTTGCGGTTGCGGCTGTTCCTCCATGAAACTTTGAACCATTTTCGCCAAGCAAACAGAGCTCGGTTCCAACTCAGCTCCTCCTCCACCTCCATCTTTGTTGTTGTTGTTGTTCTTCGGAAACTGTTTCTCAAACGCGAAGAATCTCCTCAAACGCCACTTCGATACTGGTTCGTTTCGAACAAGTCCCGTAACTTTCTCTGAATCTATGTCAATCGGTTGGATCTTCAATGGAGCCATTAAAACTCTCAACAAAAACAATTACTCTATCTCTCTCTACGAACTATACTTTTTTTGGAGCTTCAAAATTCAGAAATTCATTATCATAGTCACGAGTCAAAATTACTAGCCAGTCAACACAGTCAATGACTCAGAGTCACAAGTTATGCATGTTCTTCACCGAAACAAAATC
TARGET=1105,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4154
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=0.194358
PRIMER_LEFT_SEQUENCE=CCTCCATGAAACTTTGAACCAT
PRIMER_RIGHT_SEQUENCE=ATTGAAGATCCAACCGATTGAC
PRIMER_LEFT=1027,22
PRIMER_RIGHT=1238,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=60.194
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.5008
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=0.279852
PRIMER_LEFT_1_SEQUENCE=CCTCCATGAAACTTTGAACCAT
PRIMER_RIGHT_1_SEQUENCE=TTAATGGCTCCATTGAAGATCC
PRIMER_LEFT_1=1027,22
PRIMER_RIGHT_1=1249,22
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=60.280
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.5265
PRIMER_LEFT_2_PENALTY=0.332137
PRIMER_RIGHT_2_PENALTY=0.194358
PRIMER_LEFT_2_SEQUENCE=TTCCTCCATGAAACTTTGAACC
PRIMER_RIGHT_2_SEQUENCE=ATTGAAGATCCAACCGATTGAC
PRIMER_LEFT_2=1025,22
PRIMER_RIGHT_2=1238,22
PRIMER_LEFT_2_TM=60.332
PRIMER_RIGHT_2_TM=60.194
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.5308
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.309780
PRIMER_LEFT_3_SEQUENCE=CCTCCATGAAACTTTGAACCAT
PRIMER_RIGHT_3_SEQUENCE=AGAAAGTTACGGGACTTGTTCG
PRIMER_LEFT_3=1027,22
PRIMER_RIGHT_3=1206,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.690
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.5365
PRIMER_LEFT_4_PENALTY=0.003166
PRIMER_RIGHT_4_PENALTY=0.533373
PRIMER_LEFT_4_SEQUENCE=GGCGACTGATTCATGTGTAAAA
PRIMER_RIGHT_4_SEQUENCE=CGAAACGAACCAGTATCGAAGT
PRIMER_LEFT_4=899,22
PRIMER_RIGHT_4=1186,22
PRIMER_LEFT_4_TM=60.003
PRIMER_RIGHT_4_TM=60.533
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_03613 Ps_contig_mira-and-tgicl-ass_franssen_30546
SEQUENCE=AAATTACATATACAAAACTCATTATTTATATTTAGAAGATGGATAATATTATTTTTATTTCATCGCGTCACTTTGCAAAGTTGGAGTTCTTCCATTTCGTTTAACCTAGCACAATCTGCCGCCGCCGCCACCGTAATTCAAGCCCTCCGCTGGAACGGTTTTTGTTATCCAGTCTGGTTTATTGACCTTGTGAAATCGGATCAAATGGAGGATGATTGGGAGGAAGAGAAGTTGGCACCTATTGAACTTAAGTTACAAGAGCCGCCTAAACTTAAATGGGAAGATGAAGATGTTGATGAAGATGATGTTAAGGATTCTTGGGAGGATGACGATGAGCCCGCTCCTGCACCTGCAGCACCTGCAGTAAAAACAACTGAAAAGGCTCCTAAAAAATCTTCTGAAAAAGCTACTGATAAGAAGGGTAAGAAAGTAGAACCAGTAAAGGAAGAACCACTGGATCCTCTAGCTGAAAAACTTCGCCAACAAAGACTAGTTGAAGAAGCAGATTACAAGTCAACTAAGGAATTGTTTGGTGGGGCCAATGATGAAAAGAACATCGATACTTTCATACCGAAATCTGAGGGTGATTTCTTGGAGTACGCAGAGCTCATCTCACACAGGCTTCGTGCTTTCGAGAAAAGTTATCATTATATGAATCTACTGAAGTCTGTAATGAGAATATCCATGACTTCTCTAAAAGGAGCCGATGCCAAAGATATTGCATCTTCCGTAACTGCCATTGCAAATGAGAAGATTAAAGCAGAGAAGGAATCCAATGCTGGTAAAAAGAAGACAGGTGGCAAGAAAAAACAGCTTAATGTTGACAAGCCAGATGAAGATTTTGTTGCCGCTGATCGATACGATGCTTTGGATGATTTTGATTTCATGTGAAAACTGTTGATTGTCTTAGTAACGTGCTTCAACTGTTTCTACATTGGAAATTGGGACAAGACCCTTCGGCATAACCTTGTTCAAAATTGAAGAGTTCTGTCCTGTATTTTGTCCAATAAGAGTGTGCATCAGTAGTGTCTTTTCTTTACATTTTTGTCACCATTATACTTGGAAGTTGGTTGATCTTTCACTAGATGATTTATACAGGTTAAAAGTTTGAAGATGAATGTGTATTAAACATTGGATATTGCTTTTACCTTGGGTACATTCATGTTGGATTGTAAGAATAGAACATGCTTTGGACTTTTTTTTTGTTACTAAAAAATGAACAGGTCTATATGTCAAGGAAATGAGTTTATATCTAATAAATAGGCAAGNTGGT
TARGET=118,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4309
PRIMER_LEFT_PENALTY=0.363436
PRIMER_RIGHT_PENALTY=0.067436
PRIMER_LEFT_SEQUENCE=GCAAAGTTGGAGTTCTTCCATT
PRIMER_RIGHT_SEQUENCE=CATCAACATCTTCATCTTCCCA
PRIMER_LEFT=74,22
PRIMER_RIGHT=297,22
PRIMER_LEFT_TM=59.637
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.4361
PRIMER_LEFT_1_PENALTY=0.368624
PRIMER_RIGHT_1_PENALTY=0.067436
PRIMER_LEFT_1_SEQUENCE=AAGTTGGAGTTCTTCCATTTCG
PRIMER_RIGHT_1_SEQUENCE=CATCAACATCTTCATCTTCCCA
PRIMER_LEFT_1=77,22
PRIMER_RIGHT_1=297,22
PRIMER_LEFT_1_TM=59.631
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.4779
PRIMER_LEFT_2_PENALTY=0.363436
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=GCAAAGTTGGAGTTCTTCCATT
PRIMER_RIGHT_2_SEQUENCE=TAGGTGCCAACTTCTCTTCCTC
PRIMER_LEFT_2=74,22
PRIMER_RIGHT_2=240,22
PRIMER_LEFT_2_TM=59.637
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.4831
PRIMER_LEFT_3_PENALTY=0.368624
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=AAGTTGGAGTTCTTCCATTTCG
PRIMER_RIGHT_3_SEQUENCE=TAGGTGCCAACTTCTCTTCCTC
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=240,22
PRIMER_LEFT_3_TM=59.631
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.5578
PRIMER_LEFT_4_PENALTY=0.363436
PRIMER_RIGHT_4_PENALTY=0.194358
PRIMER_LEFT_4_SEQUENCE=GCAAAGTTGGAGTTCTTCCATT
PRIMER_RIGHT_4_SEQUENCE=GATCCGATTTCACAAGGTCAAT
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=201,22
PRIMER_LEFT_4_TM=59.637
PRIMER_RIGHT_4_TM=60.194
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_03614 Ps_contig_mira-and-tgicl-ass_franssen_30552
SEQUENCE=CACCACAAACAACTTAAACCTTTTTCTCTCTCTCTATCTCTATCGACAAATTCGTTTTTTTTCATCTTGGGTTTTTGTTAAATTGGATAAAGATCTGGTTTTTAGGTTTGATTTGTGTGAAAGGCTTTGTTTTTCGATCTGGGTGTGAGGGAAATTGGTGTGTTGTGTTGATAATGAGGAAAGAGAGAGGGAAAAACCTTGTGGGTATGTGGGCACTATCACAAATATGAAGAAGGTGAAGTTTGTCCGATTTGTGGTCATAGGGTTCCTGTTGGATCGGGAAGAAAAAGTTCGATAAC
TARGET=25,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03615 Ps_contig_mira-and-tgicl-ass_franssen_30553
SEQUENCE=CGTTCTAATTGTATATACTTCTATAGATAGAATTTCACTTGTAACAGAAAAAAGAAAAGAACTTGATAAAGTACTAAGCTTTGATATAACTCAATATTACAACCATCATAATGATTGATAACTATGAAAATATTTAAAATATGATGGGAAGAAAAAACTATAGAATTGACAAGTTCACTTATAATTGTTTGGTCCTGTAATAAACCTTACCACAAATCCAAATGTTTAGAATCAAGATCCATCCATTTGAATATCAGTAACATTCGGATTCGGATTAGGTGGATTTGTATTGAAAGGATTTCCAGTAACATTCTGACCGGCTCCGAATGTGAACTCGGTAGGAGCTATATCGAGGGGTGCTCGACTGAAAATTGACGGAGTTCCGGCGCAGTTGAATGCGGGAAAGGCGGGAAGTGGAGGTAGATCGTTGGGTTTCGGGAAGCCGAAGCTAATTGAAGATGGCCCTGCTGTAGGTGAGAAAGCGGAAGGCAGAAGAAAAGAGTTTCCGGCTGATCCGAAAAGAGTTTTCTCGTATTCCTGTAGATGCTGGTAGGCAGCTCCGGATAATTCGACGGATGGTCTGCGTTCCTTTACCCACTGATAACTCTGTGCGAGTCTCCATCCTCTCGACTTCATCAAGTAGCCGATCACAACAGCCGGGGACCGACTCTTTCCTGACATGCAATGAACCAGAACACGCTCCTTATCCTTCTCACATTGCTCGAGAAACTGGTTTGCTTCATCAAATTGGAAACTTTTGTCATCCGGGAGGCAGTGATATGTAAACGAGTTCTTGTAGAGATTCTGACAAGAAGGGACAGTATTGAGGATACGTGAAATCCCCTGAGTCTTGAGAAGCTCAGAGCGTGAAGCGTTATCATAGCTCCCCAAGTAAAGAAACTCCGGCAAGATCACAGAAGGAAAAGCACTAACTTGTATCGAACTTTTCTCCGATCCAACAGGAACCCTATGACCACAAATCGGACAAACTTCACCTTCTTCATATTTGTGATAGTGCCCACATACCCCACAAGGGTTTTCCCTCTCTCTTTTCCTCATTATCAACACAACACACCAATTTCCCTCCCACCCAGATCGAAAAACAAAGCCTTTCACACAAATCAAACCTAAAAACCAGATCTTTATCCAATTTAACAAAAACCCAGATGAAAAAAAAAACGATTTGCGATAGAGATAGAGAGAGAGAAAAAAAGGGTTTTGTTGTTTTGTGGTGACAGCACAGCAAGAGAAGGAAAAGATAAAGAAACAAGGAAAGGGTCAAATTTAAGTAAAGGTAAGTTTTTCCTTTTTCTTTTTACTTTAATTGTTTATGT
TARGET=1197,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2781
PRIMER_LEFT_PENALTY=0.093026
PRIMER_RIGHT_PENALTY=0.185055
PRIMER_LEFT_SEQUENCE=ACAAATCGGACAAACTTCACCT
PRIMER_RIGHT_SEQUENCE=CTTTTCCTTCTCTTGCTGTGCT
PRIMER_LEFT=975,22
PRIMER_RIGHT=1257,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.185
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.2871
PRIMER_LEFT_1_PENALTY=0.102004
PRIMER_RIGHT_1_PENALTY=0.185055
PRIMER_LEFT_1_SEQUENCE=CAAGGGTTTTCCCTCTCTCTTT
PRIMER_RIGHT_1_SEQUENCE=CTTTTCCTTCTCTTGCTGTGCT
PRIMER_LEFT_1=1030,22
PRIMER_RIGHT_1=1257,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.185
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=0.2946
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.185055
PRIMER_LEFT_2_SEQUENCE=CAGGAACCCTATGACCACAAAT
PRIMER_RIGHT_2_SEQUENCE=CTTTTCCTTCTCTTGCTGTGCT
PRIMER_LEFT_2=959,22
PRIMER_RIGHT_2=1257,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.185
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=0.3242
PRIMER_LEFT_3_PENALTY=0.139129
PRIMER_RIGHT_3_PENALTY=0.185055
PRIMER_LEFT_3_SEQUENCE=CTATGACCACAAATCGGACAAA
PRIMER_RIGHT_3_SEQUENCE=CTTTTCCTTCTCTTGCTGTGCT
PRIMER_LEFT_3=967,22
PRIMER_RIGHT_3=1257,22
PRIMER_LEFT_3_TM=59.861
PRIMER_RIGHT_3_TM=60.185
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.4550
PRIMER_LEFT_4_PENALTY=0.269968
PRIMER_RIGHT_4_PENALTY=0.185055
PRIMER_LEFT_4_SEQUENCE=TATGACCACAAATCGGACAAAC
PRIMER_RIGHT_4_SEQUENCE=CTTTTCCTTCTCTTGCTGTGCT
PRIMER_LEFT_4=968,22
PRIMER_RIGHT_4=1257,22
PRIMER_LEFT_4_TM=59.730
PRIMER_RIGHT_4_TM=60.185
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_03616 Ps_contig_mira-and-tgicl-ass_franssen_30554
SEQUENCE=AGANNACANGGGGAACAACGTTAACATTTCTCTTCTTCTTCTGCAATTTCATCAATAACATCAAATTCTCGTTCTCCTCACTTCATTTTTTGTCTCTGTTATTTTTCTTTAGTTTCATTTCGTTTTTTCTGATATTAAAATGGCTGAAACAAAAAACACTCATTGCATTGTTGAAATCCCAGTAGACCAAGAACATTCAGAGACCAACAACAAGGCTTTGTTGTGTTCCAACATGTTCGAGGCGATTGATGATCATCCATTAACCGAAATCTCTGAATCTCCCGGTCACCTCTTACTCTTAAAGCTATGGCAAAGAGAAGAAAATCTCTTCGCCAAACGTATTTCAAGAAAAGAAACCAGAATGGATTCAATAAAATCGGAACTTTTTCAACTCTCTTCGTTTTTCTTCATCTTTCATGGCTTTTTCTTAACACTGTTGTTTACTTCATGGGCTAAAGCCGAACAGGTCCAAAAGGGTGGTGGTGGTAGTTGTAAGATTTGGTGGATACCTTCGATGATTTCTCTTTGCACTTCGCTTGTGTTTGTGTTTTTGGTTCAAGTGAAGTTGTATAGGTATTGGAAAGTGTGGGAACAGTTACAGAGGGAGAGGAATGATAGTAGAGGTGTTGGGAGGTGTATTCAGGAGTTGAGGATGAAAGGTGCTAGCTTTGATTTGTCGAAGGAGTTGGGTTTGAATGGGAAGAGGATGAAGAGTTCGAGTGTTGAGATTAAATGGAAGGTGGTTACTTGGTTTTCTCGGTATTTGATTACTGTTTGTCTTGTTTGTTTTACAGGTTTGGCTTTTCCTGCTTCCAAGTTTGTTCTATGTGGCTTGTGAGGATGATGATGATGATGTTGATGATAATGATGGAGAGCTTGGATTTGTTCATTGTTAATGATTATGCCTCTGCTAAAGGAATGAAGCATTGTTGGTCCGATTTTAGAAAACCCTTTTGAAGCACAAAAAATGGCCTCAAGAGGTTTCAATGTAAACTACAAATTTAGCAAGGATGGGAATGAAGCATTATCTATGGAGACAGGGATTCTGCAAGAGCATTGATGAATATGTGTCATTGTGAATACTGACTAGAGATTCTGCAAGAGCATTGATGAATATGTGTCATTGTGAATACTGACTAGAGATTCTGCAAGAGCGTGGTTCTGTTATGATGCAACACATCTTGTGTGCCTTTGGCATCTACAATTTTGTAAGCACTGTAATGCAGCTATGCTACTACGAGCTGCTTCAATTTTGATTCTATTTTTTGAACATTACAAGTTCTCTGAACTACTAATGCTGTTGCTGTTATACTCAGCTGCTTCAATTTTGATACAATCTTGTGAACACTAATGCAGCTGTTTGTACGTACTGCCAGCTGCTTCAATTTTGTGAATACTGCAGCTGTATTTATGCTGCGAGCGGCTTA
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03617 Ps_contig_mira-and-tgicl-ass_franssen_30554
SEQUENCE=AGANNACANGGGGAACAACGTTAACATTTCTCTTCTTCTTCTGCAATTTCATCAATAACATCAAATTCTCGTTCTCCTCACTTCATTTTTTGTCTCTGTTATTTTTCTTTAGTTTCATTTCGTTTTTTCTGATATTAAAATGGCTGAAACAAAAAACACTCATTGCATTGTTGAAATCCCAGTAGACCAAGAACATTCAGAGACCAACAACAAGGCTTTGTTGTGTTCCAACATGTTCGAGGCGATTGATGATCATCCATTAACCGAAATCTCTGAATCTCCCGGTCACCTCTTACTCTTAAAGCTATGGCAAAGAGAAGAAAATCTCTTCGCCAAACGTATTTCAAGAAAAGAAACCAGAATGGATTCAATAAAATCGGAACTTTTTCAACTCTCTTCGTTTTTCTTCATCTTTCATGGCTTTTTCTTAACACTGTTGTTTACTTCATGGGCTAAAGCCGAACAGGTCCAAAAGGGTGGTGGTGGTAGTTGTAAGATTTGGTGGATACCTTCGATGATTTCTCTTTGCACTTCGCTTGTGTTTGTGTTTTTGGTTCAAGTGAAGTTGTATAGGTATTGGAAAGTGTGGGAACAGTTACAGAGGGAGAGGAATGATAGTAGAGGTGTTGGGAGGTGTATTCAGGAGTTGAGGATGAAAGGTGCTAGCTTTGATTTGTCGAAGGAGTTGGGTTTGAATGGGAAGAGGATGAAGAGTTCGAGTGTTGAGATTAAATGGAAGGTGGTTACTTGGTTTTCTCGGTATTTGATTACTGTTTGTCTTGTTTGTTTTACAGGTTTGGCTTTTCCTGCTTCCAAGTTTGTTCTATGTGGCTTGTGAGGATGATGATGATGATGTTGATGATAATGATGGAGAGCTTGGATTTGTTCATTGTTAATGATTATGCCTCTGCTAAAGGAATGAAGCATTGTTGGTCCGATTTTAGAAAACCCTTTTGAAGCACAAAAAATGGCCTCAAGAGGTTTCAATGTAAACTACAAATTTAGCAAGGATGGGAATGAAGCATTATCTATGGAGACAGGGATTCTGCAAGAGCATTGATGAATATGTGTCATTGTGAATACTGACTAGAGATTCTGCAAGAGCATTGATGAATATGTGTCATTGTGAATACTGACTAGAGATTCTGCAAGAGCGTGGTTCTGTTATGATGCAACACATCTTGTGTGCCTTTGGCATCTACAATTTTGTAAGCACTGTAATGCAGCTATGCTACTACGAGCTGCTTCAATTTTGATTCTATTTTTTGAACATTACAAGTTCTCTGAACTACTAATGCTGTTGCTGTTATACTCAGCTGCTTCAATTTTGATACAATCTTGTGAACACTAATGCAGCTGTTTGTACGTACTGCCAGCTGCTTCAATTTTGTGAATACTGCAGCTGTATTTATGCTGCGAGCGGCTTA
TARGET=476,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1510
PRIMER_LEFT_PENALTY=0.123278
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=TCCCGGTCACCTCTTACTCTTA
PRIMER_RIGHT_SEQUENCE=AAGCGAAGTGCAAAGAGAAATC
PRIMER_LEFT=279,22
PRIMER_RIGHT=537,22
PRIMER_LEFT_TM=60.123
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=0.2879
PRIMER_LEFT_1_PENALTY=0.123278
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=TCCCGGTCACCTCTTACTCTTA
PRIMER_RIGHT_1_SEQUENCE=TCGAAGGTATCCACCAAATCTT
PRIMER_LEFT_1=279,22
PRIMER_RIGHT_1=514,22
PRIMER_LEFT_1_TM=60.123
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.3689
PRIMER_LEFT_2_PENALTY=0.123278
PRIMER_RIGHT_2_PENALTY=0.245661
PRIMER_LEFT_2_SEQUENCE=TCCCGGTCACCTCTTACTCTTA
PRIMER_RIGHT_2_SEQUENCE=AAACACAAACACAAGCGAAGTG
PRIMER_LEFT_2=279,22
PRIMER_RIGHT_2=549,22
PRIMER_LEFT_2_TM=60.123
PRIMER_RIGHT_2_TM=60.246
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=0.3959
PRIMER_LEFT_3_PENALTY=0.364827
PRIMER_RIGHT_3_PENALTY=0.031122
PRIMER_LEFT_3_SEQUENCE=ATCTCTTCGCCAAACGTATTTC
PRIMER_RIGHT_3_SEQUENCE=CTCCCTCTGTAACTGTTCCCAC
PRIMER_LEFT_3=323,22
PRIMER_RIGHT_3=606,22
PRIMER_LEFT_3_TM=59.635
PRIMER_RIGHT_3_TM=60.031
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.3966
PRIMER_LEFT_4_PENALTY=0.368866
PRIMER_RIGHT_4_PENALTY=0.027715
PRIMER_LEFT_4_SEQUENCE=AATCTCCCGGTCACCTCTTACT
PRIMER_RIGHT_4_SEQUENCE=AAGCGAAGTGCAAAGAGAAATC
PRIMER_LEFT_4=275,22
PRIMER_RIGHT_4=537,22
PRIMER_LEFT_4_TM=60.369
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_03618 Ps_contig_mira-and-tgicl-ass_franssen_30554
SEQUENCE=AGANNACANGGGGAACAACGTTAACATTTCTCTTCTTCTTCTGCAATTTCATCAATAACATCAAATTCTCGTTCTCCTCACTTCATTTTTTGTCTCTGTTATTTTTCTTTAGTTTCATTTCGTTTTTTCTGATATTAAAATGGCTGAAACAAAAAACACTCATTGCATTGTTGAAATCCCAGTAGACCAAGAACATTCAGAGACCAACAACAAGGCTTTGTTGTGTTCCAACATGTTCGAGGCGATTGATGATCATCCATTAACCGAAATCTCTGAATCTCCCGGTCACCTCTTACTCTTAAAGCTATGGCAAAGAGAAGAAAATCTCTTCGCCAAACGTATTTCAAGAAAAGAAACCAGAATGGATTCAATAAAATCGGAACTTTTTCAACTCTCTTCGTTTTTCTTCATCTTTCATGGCTTTTTCTTAACACTGTTGTTTACTTCATGGGCTAAAGCCGAACAGGTCCAAAAGGGTGGTGGTGGTAGTTGTAAGATTTGGTGGATACCTTCGATGATTTCTCTTTGCACTTCGCTTGTGTTTGTGTTTTTGGTTCAAGTGAAGTTGTATAGGTATTGGAAAGTGTGGGAACAGTTACAGAGGGAGAGGAATGATAGTAGAGGTGTTGGGAGGTGTATTCAGGAGTTGAGGATGAAAGGTGCTAGCTTTGATTTGTCGAAGGAGTTGGGTTTGAATGGGAAGAGGATGAAGAGTTCGAGTGTTGAGATTAAATGGAAGGTGGTTACTTGGTTTTCTCGGTATTTGATTACTGTTTGTCTTGTTTGTTTTACAGGTTTGGCTTTTCCTGCTTCCAAGTTTGTTCTATGTGGCTTGTGAGGATGATGATGATGATGTTGATGATAATGATGGAGAGCTTGGATTTGTTCATTGTTAATGATTATGCCTCTGCTAAAGGAATGAAGCATTGTTGGTCCGATTTTAGAAAACCCTTTTGAAGCACAAAAAATGGCCTCAAGAGGTTTCAATGTAAACTACAAATTTAGCAAGGATGGGAATGAAGCATTATCTATGGAGACAGGGATTCTGCAAGAGCATTGATGAATATGTGTCATTGTGAATACTGACTAGAGATTCTGCAAGAGCATTGATGAATATGTGTCATTGTGAATACTGACTAGAGATTCTGCAAGAGCGTGGTTCTGTTATGATGCAACACATCTTGTGTGCCTTTGGCATCTACAATTTTGTAAGCACTGTAATGCAGCTATGCTACTACGAGCTGCTTCAATTTTGATTCTATTTTTTGAACATTACAAGTTCTCTGAACTACTAATGCTGTTGCTGTTATACTCAGCTGCTTCAATTTTGATACAATCTTGTGAACACTAATGCAGCTGTTTGTACGTACTGCCAGCTGCTTCAATTTTGTGAATACTGCAGCTGTATTTATGCTGCGAGCGGCTTA
TARGET=840,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1678
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=TTGGGAGGTGTATTCAGGAGTT
PRIMER_RIGHT_SEQUENCE=ACAAATCCAAGCTCTCCATCAT
PRIMER_LEFT=626,22
PRIMER_RIGHT=885,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.2175
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.081468
PRIMER_LEFT_1_SEQUENCE=TTGGGAGGTGTATTCAGGAGTT
PRIMER_RIGHT_1_SEQUENCE=GAACAAATCCAAGCTCTCCATC
PRIMER_LEFT_1=626,22
PRIMER_RIGHT_1=887,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.3128
PRIMER_LEFT_2_PENALTY=0.281044
PRIMER_RIGHT_2_PENALTY=0.031769
PRIMER_LEFT_2_SEQUENCE=AGGTGTTGGGAGGTGTATTCAG
PRIMER_RIGHT_2_SEQUENCE=ACAAATCCAAGCTCTCCATCAT
PRIMER_LEFT_2=621,22
PRIMER_RIGHT_2=885,22
PRIMER_LEFT_2_TM=60.281
PRIMER_RIGHT_2_TM=59.968
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.3625
PRIMER_LEFT_3_PENALTY=0.281044
PRIMER_RIGHT_3_PENALTY=0.081468
PRIMER_LEFT_3_SEQUENCE=AGGTGTTGGGAGGTGTATTCAG
PRIMER_RIGHT_3_SEQUENCE=GAACAAATCCAAGCTCTCCATC
PRIMER_LEFT_3=621,22
PRIMER_RIGHT_3=887,22
PRIMER_LEFT_3_TM=60.281
PRIMER_RIGHT_3_TM=60.081
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.3932
PRIMER_LEFT_4_PENALTY=0.361394
PRIMER_RIGHT_4_PENALTY=0.031769
PRIMER_LEFT_4_SEQUENCE=GGGAGGTGTATTCAGGAGTTGA
PRIMER_RIGHT_4_SEQUENCE=ACAAATCCAAGCTCTCCATCAT
PRIMER_LEFT_4=628,22
PRIMER_RIGHT_4=885,22
PRIMER_LEFT_4_TM=60.361
PRIMER_RIGHT_4_TM=59.968
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_03619 Ps_contig_mira-and-tgicl-ass_franssen_30559
SEQUENCE=AAATTAAAGTCNNRGYATTTTTTAATGATGGTGTCAAGTTTATGTTACTTTGTGACAAATGGAGGGTCCTTAGTTTTGTTTCTTCTCTTCTTTCTCTTTGTTCTTCTCTCAACAATTCTCTAATCACTCTCACTCTTCTTCTTCTTCTTCACCCTTCTGATATTGCTCATTCTTCAGGTTATTGAAAGCTTTTTTCTTGTTTGAAAGTTTGAATTCATTGTGATGACTCTACCAAGGCTAGGAATAGGGGATGATGAAGGTGAAAGCAATGTTACTTTGTTGGTTTCTTCATCAGCCTCAATGGAAAAAAGTGTGTGTTTGAATTTGAATGGTCCAAAACCAAAAGAGTTTAACTACATGGGGTTATCATGTGATTCTTCATCATCAGTGGATAGTTTGGTTACAAAGATTCAATCTTTTGAAGATGGGGCCAAAAGAAACTTGAACCTTAAAGCTACTGAGCTTAGATTGGGACTTCCAGGCTCAGTGTCTCCTGAAAGGGATTCTTCGGATCTTTGTTTGAGAAGTTTTGATGAAAAGCCTCTTTTTCCTTTGCACCCTCAAAAAGATGAATCAAAGCATGCTGTTTTAGGTAACAAAAGAGGGTTTTCTGATGCCATGAATGTTTTCTCAGAGGGGAAAATGAAGCCTGGCTCCAATATGCTTGAGAATGTTGCGGCGCTGCAAGGGAAAGGCACTGAAATAGCAACAGTTCAGGTTGGTCTTGAGAGGCCGAACGGTGTCGGTGAAAGCAGACCAAATCTTAATGGTTCTGCAAATAATGGAAATAGCATTGCTCCAGCTACCAAGGCACAGGTTGTTGGTTGGCCTCCAATAAGATCATTTCGCAAAAATTCCTTGACAACTGCTTCAAAGAACACCGAAGAGGCTGATGGAAAACTGGGATTAGGAGGCGCCGTGTTTGTGAAGGTTAGCATGGATGGTGCTCCTTATTTGAGAAAAGTAGATCTGAAGAACTACACTGCATATTCAGAACTCTCTTCATCTCTTGAGAAGATGTTCAGCTGTTTCACCATAGGACAATGTGAATCTCATGGAAATCAGATGCTGAATGAAACAAAGCTGAGAGATCTGCTTCATGGGTCAGAATATGTTATTACTTACGAGGATAAAGACGGTGACTGGATGCTTGTCGGTGATGTTCCTTGGGAGATGTTTATCGATACATGCAGGAGGCTAAGGATCATGAAAAGCTCCGAAGCGATTGGTCTAGCACCTAGAGCAGTTGAAAAAAGTAAAAGCAGGAACTAGGAAGTATCAATAATGGTCACACATTCTATCCATATATCTATGGTGTGAAAATGGTTATGTGAAGCTTTTATTATTTTTTATTATTGTGACAATTTATATAATCCTAAGACATGTTAGTGTGAAAGATTCTTATATATCCTGTTGTGAAGGATTAGTGTGTGAAATATTGTGAATTGCATTATATATCTATATATATGTGTGTGGTTTTGTGTGATGAAAACAAAACTATATATAACTTAATATGGAATGTAACTTTTAACNGAAC
TARGET=134,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03620 Ps_contig_mira-and-tgicl-ass_franssen_30564
SEQUENCE=TTCCTTCCATTTTCTTCGCTCTATAATCACCAAATCCAAATCCCTCCATTTCCATTCACCATCCCATTCTATTTCTCACCCACCCTTTTCCTCCTACACCTCCTCCTCCTCAAACCATNACAAACACAACCACTTTCACTTCCAAACCTCTGTATACCTAAAACCTGTTTAGATTCTCTTTTCATTTTCTGTACTTCAATTGCACTCTCTTTCACTCTCTTCATTTCAAATCCTGATTCAGCTTCTGCTTTTGTTGTAACCACACCAAGGAAGTTGCAGACCGATGAACTAGCTACTGTTCGTCTCTTTCAAGAGAATACTCCTTCTGTTGTTTATATAACTAATCTTGCTGTCAAGCAGGATGCGTTTACTCTGGACGTGTTGGAGGTTCCTCAAGGATCAGGGTCGGGTTTCGTTTGGGATAAGGATGGTCATATAGTCACCAATTATCATGTTATCAGGGGTGCTTCTGATCTCAGGGTCACTCTTGCAGACCAATCAACTTATGATGCAAAAGTTGTTGGTTTTGACCAAGACAAGGACGTTGCCGTGCTTCGCGTTGATGCGCCTAAAGAGAAGCTAAGACCTATACCTGTTGGGGTCTCTGCGGACTTGCTCGTTGGTCAGAAAGTGTATGCTATTGGGAACCCGTTTGGACTTGACCACACTCTTACAACTGGAGTCATCAGTGGACTTCGGCGAGAAATTAGTTCGGCTGCAACTGGTCGTCCTATTCAAGATGTTATACAGACCGACGCAGCAATTAATCCTGGTAACAGTGGAGGGCCACTCCTTGATAGTTCTGGAAGCCTCATTGGGATTAATACAGCTATATACTCCCCTTCTGGTGCATCCTCCGGTGTTGGATTTTCTATTCCTGTCGATACTGTAAATGGCATTGTTGATCAATTGGTGAAGTTTGGGAAGGTTACTAGACCTATATTAGGGATCAAGTTTGCTCCTGACCAATCTGTGGAGCAATTGGGAGTAAGTGGAGTGCTTGTCTTGGATGCTCCTGCCACTGGTCCAGCTGGAAAAGCAGGCTTACAATCGACAAAGCGTGATGCCTACGGGAGACTCATTTTAGGAGACATCATAACATCTGTGAATGGTAAGAAGGTTACTAATGGAAGCGACTTGTACAGAATTCTTGATCAATGTGAAGTTGGCGAGAAGGTGATTGTGGAGGTTTTGCGAGGTGACCACAAAGAAAAGATCCCGGTCGTTCTCGAGCCAAAGGCCGACGAATCATGATGTGCAACTCATTCTACATCATTCATGTATATATACACATTTTATTTGGCAGGGAATGCAGCTGTTTTATGTGCATTCTTAGGTTGTTTCCCAATTGTGTACATACTGTACTACCTCTCCAAGATAAGTTGAGATTGAACAAAAGAAAAGCACCAGAAGAAACATTATATGCTTATTATTGTTGTGAAGAATAGATTCATTCGCTTACATGGATTTTTCTCAAGT
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1298
PRIMER_LEFT_PENALTY=0.090580
PRIMER_RIGHT_PENALTY=0.039178
PRIMER_LEFT_SEQUENCE=CCATTCACCATCCCATTCTATT
PRIMER_RIGHT_SEQUENCE=TAGCTAGTTCATCGGTCTGCAA
PRIMER_LEFT=51,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.909
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.1613
PRIMER_LEFT_1_PENALTY=0.090580
PRIMER_RIGHT_1_PENALTY=0.070748
PRIMER_LEFT_1_SEQUENCE=CCATTCACCATCCCATTCTATT
PRIMER_RIGHT_1_SEQUENCE=CAACTTCCTTGGTGTGGTTACA
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=275,22
PRIMER_LEFT_1_TM=59.909
PRIMER_RIGHT_1_TM=59.929
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=0.2261
PRIMER_LEFT_2_PENALTY=0.186949
PRIMER_RIGHT_2_PENALTY=0.039178
PRIMER_LEFT_2_SEQUENCE=TCCCATTCTATTTCTCACCCAC
PRIMER_RIGHT_2_SEQUENCE=TAGCTAGTTCATCGGTCTGCAA
PRIMER_LEFT_2=61,22
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=60.187
PRIMER_RIGHT_2_TM=60.039
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=234
PRIMER_PAIR_PENALTY_3=0.3635
PRIMER_LEFT_3_PENALTY=0.324355
PRIMER_RIGHT_3_PENALTY=0.039178
PRIMER_LEFT_3_SEQUENCE=TCCTTCCATTTTCTTCGCTCTA
PRIMER_RIGHT_3_SEQUENCE=TAGCTAGTTCATCGGTCTGCAA
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=60.324
PRIMER_RIGHT_3_TM=60.039
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=0.3695
PRIMER_LEFT_4_PENALTY=0.324355
PRIMER_RIGHT_4_PENALTY=0.045112
PRIMER_LEFT_4_SEQUENCE=TCCTTCCATTTTCTTCGCTCTA
PRIMER_RIGHT_4_SEQUENCE=TTTGGAAGTGAAAGTGGTTGTG
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=145,22
PRIMER_LEFT_4_TM=60.324
PRIMER_RIGHT_4_TM=60.045
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_03621 Ps_contig_mira-and-tgicl-ass_franssen_30565
SEQUENCE=CGGGTAGAACATGTCACGTTGAAAACGTAATCCAACATTCCAAGAACCACAAACAACGTTACTCCACTAAAAAAACAATGGCCATTGCTTCTTCTTCCTCTTCCCTCATTTCCACATTCTTCTTCCATTCTTCCTCATTTTCTCGCTCTATAATCACCAAATCCAAATCCCTCCATTTCCATTCACCATCCCATTCTATTTCTCACCCTACCCTTTTCCTCCTACACCCTCCTCCTCCTCAAACCCCATTAACCAAAACACAACCACTTTCACTTCCAAACCTCTGTATACCTAAAACCTGTTTAGATTCTCTTTTCATTTTCTGTACTTCAATTGCACTCTCTTTCACTCTCTT
TARGET=228,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1406
PRIMER_LEFT_PENALTY=0.095538
PRIMER_RIGHT_PENALTY=0.045112
PRIMER_LEFT_SEQUENCE=TGTCACGTTGAAAACGTAATCC
PRIMER_RIGHT_SEQUENCE=TTTGGAAGTGAAAGTGGTTGTG
PRIMER_LEFT=11,22
PRIMER_RIGHT=279,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=60.045
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=269
PRIMER_PAIR_PENALTY_1=0.1676
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=0.107763
PRIMER_LEFT_1_SEQUENCE=TCCCTCATTTCCACATTCTTCT
PRIMER_RIGHT_1_SEQUENCE=GTTGTGTTTTGGTTAATGGGGT
PRIMER_LEFT_1=101,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=10.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.1676
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.107763
PRIMER_LEFT_2_SEQUENCE=TCTTCCCTCATTTCCACATTCT
PRIMER_RIGHT_2_SEQUENCE=GTTGTGTTTTGGTTAATGGGGT
PRIMER_LEFT_2=98,22
PRIMER_RIGHT_2=263,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=10.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=0.1676
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.107763
PRIMER_LEFT_3_SEQUENCE=CTCTTCCCTCATTTCCACATTC
PRIMER_RIGHT_3_SEQUENCE=GTTGTGTTTTGGTTAATGGGGT
PRIMER_LEFT_3=97,22
PRIMER_RIGHT_3=263,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=10.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=167
PRIMER_PAIR_PENALTY_4=0.1887
PRIMER_LEFT_4_PENALTY=0.143555
PRIMER_RIGHT_4_PENALTY=0.045112
PRIMER_LEFT_4_SEQUENCE=CATTCTTCCTCATTTTCTCGCT
PRIMER_RIGHT_4_SEQUENCE=TTTGGAAGTGAAAGTGGTTGTG
PRIMER_LEFT_4=125,22
PRIMER_RIGHT_4=279,22
PRIMER_LEFT_4_TM=59.856
PRIMER_RIGHT_4_TM=60.045
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_03622 Ps_contig_mira-and-tgicl-ass_franssen_30567
SEQUENCE=TACAATCCATAACTTTTCTCCAGTTTCTGAAGTTTCTGGTTGAGGAAGATCCATGTCATCATGACATGAGGAGCAATCCTGACACAATAGACAGGGAATCCGGTGTAAAATTTGAAGGGTCCTCCTGCTTTGAAGGTTTTGACAGCACAGTCAAAGAGAGCCAGTGTATGGATTAATTTTCCTTCAGCATCAGGTTGCATCTTCTGAATTTGAGTCTTCACATAATCGAATGGCAGACTGACAAGCTGCTAGAACGAAAACTCCAAGTAACTTAACCTAACCTAACCTAACGACCCAAACC
TARGET=273,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03623 Ps_contig_mira-and-tgicl-ass_franssen_30580
SEQUENCE=AGACAGAATATATACAAATAGTTGGTTCTGCTGAACTCTCTAAGTTAGCTGATAACGCTTTTTTGGCTCAAAGGATTTCGTCTATTAATGCTATGTCGGCTTTATGCGAGTCTACTGGTGCAGATATTTCACAGGTTTCTTGTGTTTTAAGTAAAAACACTAAACTTGGTTCTAGATACTTAAATGCTAGTGTTGGTTTTGGTGGTTCTTGTTTTCAAAAAGATATCCTTAACTTGGTATATATATGCGAGTGTAATGGACTCGTCGAAGTCGCTAATTATTGGAAAGAGGTGATTAAGGTGAATGACTATCAAAAGAGTAGGTTTGTGAAAAAGGTTATAACATCTATGTTTAATACAGTTTCAGGTAAGAAGATTGCTGTTCTAGGATTTTCCTTTAAGAAAGATACTAGTGATACTAGGAAAACTCCTGCGATCGATGTTTGCAAAGGACTTTTGGCAGATAACGCTTGTTTGAGCATTTACGATCCGCGGGTTAGTGAAGAACAGATTCGAAGGGATTTGTCTATGAATGAGATTCCATGGGAGAATTCAATCATGGCGGAGGCAATGAGGTTCGATGGTACGAAACAGGTTAGTGTGTATGAGGATGTTTATGAGGCGACAAAAGATGCTCACGGGATATGTGTTCTTACAGAATGGGATGAGTTCAAGGAAATTGATTATCAAAGAGTGTTTGAAAGCATGGAGAAGCCAGCATTTGTTTTTGATGGTAGAAATGTTTTGGATGTTGATAAGTTGAGGGAGATTGGGTTTATTGTGTACTCAATTGGGAAGCCATTGAAGCAGTGGCAGAGTTGCTAGATTGTGTTTTGTTGAAATAAAAATGTCATTGCCTTGAGGTTATATATATATATTGTATGAGACAATTAAGGTCATTCTTATGTTTCATTGAGAGTGAAAATTCATATAAAAAGGAATAACTACTTTACTTTTACTGATGACTAAATTTGACTCCGATTGAATATTGTTCCTTTAGTTTTTTCATTGTTCTATTATGCTATTTAACNTCTTCAATAAAGACAACAATAGGTAAA
TARGET=865,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5075
PRIMER_LEFT_PENALTY=0.057411
PRIMER_RIGHT_PENALTY=2.450072
PRIMER_LEFT_SEQUENCE=TGGCAGAGTTGCTAGATTGTGT
PRIMER_RIGHT_SEQUENCE=GGAACAATATTCAATCGGAGTC
PRIMER_LEFT=809,22
PRIMER_RIGHT=993,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=57.550
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=2.5075
PRIMER_LEFT_1_PENALTY=0.057411
PRIMER_RIGHT_1_PENALTY=2.450072
PRIMER_LEFT_1_SEQUENCE=GTGGCAGAGTTGCTAGATTGTG
PRIMER_RIGHT_1_SEQUENCE=GGAACAATATTCAATCGGAGTC
PRIMER_LEFT_1=808,22
PRIMER_RIGHT_1=993,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=57.550
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=2.5241
PRIMER_LEFT_2_PENALTY=0.073989
PRIMER_RIGHT_2_PENALTY=2.450072
PRIMER_LEFT_2_SEQUENCE=CAGTGGCAGAGTTGCTAGATTG
PRIMER_RIGHT_2_SEQUENCE=GGAACAATATTCAATCGGAGTC
PRIMER_LEFT_2=806,22
PRIMER_RIGHT_2=993,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=57.550
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=2.6268
PRIMER_LEFT_3_PENALTY=0.176730
PRIMER_RIGHT_3_PENALTY=2.450072
PRIMER_LEFT_3_SEQUENCE=TTGAGGGAGATTGGGTTTATTG
PRIMER_RIGHT_3_SEQUENCE=GGAACAATATTCAATCGGAGTC
PRIMER_LEFT_3=758,22
PRIMER_RIGHT_3=993,22
PRIMER_LEFT_3_TM=60.177
PRIMER_RIGHT_3_TM=57.550
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=2.6991
PRIMER_LEFT_4_PENALTY=0.249074
PRIMER_RIGHT_4_PENALTY=2.450072
PRIMER_LEFT_4_SEQUENCE=GTGTACTCAATTGGGAAGCCAT
PRIMER_RIGHT_4_SEQUENCE=GGAACAATATTCAATCGGAGTC
PRIMER_LEFT_4=779,22
PRIMER_RIGHT_4=993,22
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=57.550
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_03624 Ps_contig_mira-and-tgicl-ass_franssen_30581
SEQUENCE=TTTATTNATGAAGTTGATGCAATCATATAATAAAATTTCAGCAACACCATAACAAGATATTGAAGATTACCCCTTATGGAGTTTGAAATTCCATTGTACATTAATAATAATAAGAATAAGAAGAAAAAACTATAAAAAGAAACAAAAGGAAGGGGGTCAATCGTAGTTGCACAATGAAATGTGCCATGTCCCTGCCATACTACTGTCTTTAGCTATCGTTGCCAGAGAAAGCAGGCAAAATGCAATTATATCTTTTGAATCATCAATCATGACAGGAAGAAAGATAAGCCTTTAACCTATGTTGAGAATGAAAGCCATCATCCAGCTCACTGACTTTATAGTACATAGCTCGAGAGTGTTAATTAAGTCTTCCCACCCTTATGAGGTGGAGTTCCAGGTCTTGATGTGTTATTGCCAAACCCACCTTTCCCCTTCTTATCGGAAGACTTAAGTATCTGGAAAGAGCACGTCAATGTGTCATCCACACTCATCATAGCACCCGCATTATCAAACTCACCACAGTAATTTGGTGCAGAGAATATAGTCACCAGCTTACGTTTGGCAAAAAACTCATATCCATCTTCTACAACCTGGTGAGCTCTACAAATCAGATCAAGATCATGGTGCTCAAGAAACTCAGCAACCTTATCAGCCCCAAATGTGAATGACACACCTCGATCATTTTCTCCCCATCCCTCGAGATCTTTATCGGGATCAGCCCAGAGAAGGTCACAGAGAAGGCCATGATCTGGCACATCAATAGGGCGAGCAATATTCCGTATCTGATCTAAGTTTTTAAGTTCGGGAGAGAGACCACCATGCATACAAAGGATCTTCTCATCAACTAGAGCAGCAACCGGTAAACAGTTAAAGCAATCAGTAAATGTCTTCCAGAGTCGAACATTGAACCTCCTCTTGCACTCATCATAGAAACCATATATGCGATTGATGGAGGCACATTCGTGGTTACCCCTGAGTAGAAAGAAGTTCTCCTTGTATTTGATTTTGTATGCAAGAAGTAAGCATATTGTCTCTATGCTCTGCTTACCACGATCAACATAATCACCAAGAAATAAATAATTGGCTTCAGGTGGGTATCCCCCATATTCGAACAGTCTTAAAAGGTCTGAAAACTGGCCGTGGACGTCTCCACAGATTTTGATAGGAGCTTCAAGCTCAAGAAGATTAGGTTGACTGAGAAAAATCTCCTTCGCCGAAGTACAAAGTTGACGAATATCCGCCTCCGTAAGATTCACTTGCTTCGTCGTTCTACCGTTCTTCGCCGATACAAGCTTCCGAATGATATCGTCAAGCAACGTATCGTCCATCTCGAACCCTCGAACCAGAGAGATAGAGAAACGGTTATAATCCAAAACGCGAAACGGAAGAGAGAGAAAACGACGAGAGTTGTATCGCCGTTTACCGGTTGTAAAACGCAGAGTTTTATAGAAGAATGAAATGAACACTTGTTATGTTTGAAGGAGGCACCGTTCTGGGGAGGATATAGGAACCCTAGCCCAGTTGTACACAATCAAAGTC
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.1049
PRIMER_LEFT_PENALTY=2.676691
PRIMER_RIGHT_PENALTY=0.428205
PRIMER_LEFT_SEQUENCE=GAAGATTACCCCTTATGGAGTTTG
PRIMER_RIGHT_SEQUENCE=CATTGTGCAACTACGATTGACC
PRIMER_LEFT=61,24
PRIMER_RIGHT=175,22
PRIMER_LEFT_TM=59.323
PRIMER_RIGHT_TM=60.428
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=3.1189
PRIMER_LEFT_1_PENALTY=2.676691
PRIMER_RIGHT_1_PENALTY=0.442180
PRIMER_LEFT_1_SEQUENCE=GAAGATTACCCCTTATGGAGTTTG
PRIMER_RIGHT_1_SEQUENCE=TGGCAACGATAGCTAAAGACAG
PRIMER_LEFT_1=61,24
PRIMER_RIGHT_1=223,22
PRIMER_LEFT_1_TM=59.323
PRIMER_RIGHT_1_TM=59.558
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=3.1189
PRIMER_LEFT_2_PENALTY=2.676691
PRIMER_RIGHT_2_PENALTY=0.442180
PRIMER_LEFT_2_SEQUENCE=GAAGATTACCCCTTATGGAGTTTG
PRIMER_RIGHT_2_SEQUENCE=CTGGCAACGATAGCTAAAGACA
PRIMER_LEFT_2=61,24
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=59.323
PRIMER_RIGHT_2_TM=59.558
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=3.2965
PRIMER_LEFT_3_PENALTY=2.868321
PRIMER_RIGHT_3_PENALTY=0.428205
PRIMER_LEFT_3_SEQUENCE=GATTACCCCTTATGGAGTTTGA
PRIMER_RIGHT_3_SEQUENCE=CATTGTGCAACTACGATTGACC
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=175,22
PRIMER_LEFT_3_TM=57.132
PRIMER_RIGHT_3_TM=60.428
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=112
PRIMER_PAIR_PENALTY_4=3.3105
PRIMER_LEFT_4_PENALTY=2.868321
PRIMER_RIGHT_4_PENALTY=0.442180
PRIMER_LEFT_4_SEQUENCE=GATTACCCCTTATGGAGTTTGA
PRIMER_RIGHT_4_SEQUENCE=TGGCAACGATAGCTAAAGACAG
PRIMER_LEFT_4=64,22
PRIMER_RIGHT_4=223,22
PRIMER_LEFT_4_TM=57.132
PRIMER_RIGHT_4_TM=59.558
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_03625 Ps_contig_mira-and-tgicl-ass_franssen_30583
SEQUENCE=CGGGGTAGGGTTCGGTTACGTTCTTACTTATTACTACTACCACTTACTTTCGTTTTGTTTTTGGTTTTCGTTTCTTCCCTTCCCTTCCTTCCTTCCTYYCTYYCNCNYTTCCTTCGTAGCTACTCTTTCTATCATCAAAATAGGTTTACTAAAAGTAGCAAAAAATACTAATCATCACTCTTCTCTTCCTTGATTTTCCTTAGTCCTTATGTTTGGTCTTGTAACCTCAGATGTGAACCAAGACAAAGCAGAATGCACAGACAAACTCATAGGTTTGGCCAATTGTCTTCCTTTTGTAAGTGGTCAGTCCAAAACACCAACCATAGATTGTTGCACTGGTGTTAAGGATGTTGTTAATAAGAGTAAAAGATGTTTGTGTATTCTTATTAAGGATCATGATGACCCTAACCTTGGTTTTAGTATTAATGTCACACTTGCTCTTAAGTTACCAGCTGATTGTAAGTCACCTACTAATGTAACTCAGTGTATTGATCTTTTGCATTTGTCACCTAAATCTCATGAGGCTAAGATATTTGAGGACTTTGAGGAAACTCTGGAGAAAAATAGTACTACCCCAGTTCCTTCAGCCAGTAATGCCACTGGAAAAGGAACAAATACAGGAGTAGGTCAAGTCAAGAGTGGTAGCAAAGAGGGAAAGGTGCTTTATGGAGTTTCACTATTTGTTTTAGCCTCCTACTTCTTCATAATTTGAAATATGATTATTCATATCAAGATGTGAACATTTTAATAAAAGAATATATTGATATATATAGGTCAATATTATCATTTGAGAACTATAGATTGAAGATAATGTATTGTCTGTTTTTTCTTTAAATTAGGGCCCATTTATCTGTGTGATTNANTTTTCCAANCTTAAGTTTGGGTAAA
TARGET=74,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03626 Ps_contig_mira-and-tgicl-ass_franssen_30583
SEQUENCE=CGGGGTAGGGTTCGGTTACGTTCTTACTTATTACTACTACCACTTACTTTCGTTTTGTTTTTGGTTTTCGTTTCTTCCCTTCCCTTCCTTCCTTCCTYYCTYYCNCNYTTCCTTCGTAGCTACTCTTTCTATCATCAAAATAGGTTTACTAAAAGTAGCAAAAAATACTAATCATCACTCTTCTCTTCCTTGATTTTCCTTAGTCCTTATGTTTGGTCTTGTAACCTCAGATGTGAACCAAGACAAAGCAGAATGCACAGACAAACTCATAGGTTTGGCCAATTGTCTTCCTTTTGTAAGTGGTCAGTCCAAAACACCAACCATAGATTGTTGCACTGGTGTTAAGGATGTTGTTAATAAGAGTAAAAGATGTTTGTGTATTCTTATTAAGGATCATGATGACCCTAACCTTGGTTTTAGTATTAATGTCACACTTGCTCTTAAGTTACCAGCTGATTGTAAGTCACCTACTAATGTAACTCAGTGTATTGATCTTTTGCATTTGTCACCTAAATCTCATGAGGCTAAGATATTTGAGGACTTTGAGGAAACTCTGGAGAAAAATAGTACTACCCCAGTTCCTTCAGCCAGTAATGCCACTGGAAAAGGAACAAATACAGGAGTAGGTCAAGTCAAGAGTGGTAGCAAAGAGGGAAAGGTGCTTTATGGAGTTTCACTATTTGTTTTAGCCTCCTACTTCTTCATAATTTGAAATATGATTATTCATATCAAGATGTGAACATTTTAATAAAAGAATATATTGATATATATAGGTCAATATTATCATTTGAGAACTATAGATTGAAGATAATGTATTGTCTGTTTTTTCTTTAAATTAGGGCCCATTTATCTGTGTGATTNANTTTTCCAANCTTAAGTTTGGGTAAA
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03627 Ps_contig_mira-and-tgicl-ass_franssen_30585
SEQUENCE=TTTAGAAATGAAGTAGGNGTTTNAGAAATCATGAAATACAATACATGTTACAACAAAATCATTTGCCACTTATCTACATAACAATGTACTCCTTCATTTGACATTTTAACAGCTAGGTTGTTGAGAGAGAATATCTACATAAAAACTGAAAACATGATTATACACATAGATCTATCATATTGCAGACACAAGGGTGCCATAAAATTTCAGTGATATAAACCTATTTCCAAATTGTAAGCACTTCTTGAAACATCTCCAACATTAGCTCCCTTTCTGCATGGCGAAAAAACGTATTTATTTCATCTCTAACATCATATATCTTACCAAAATCAAATAAGAACGCGAAACATCCTTTCTTCAACTTGTGCAGCTGGTACATCCATGCCTCATTTAGTCTATCCAGAACATTTCCCGAGTTAAGATCTTCGAGTAGGCTTTCCTCGCACGCATCTTTCAAATCACTGATTTCGTATTTATTGGCTGCTCCAAGCAATGCAAGCCTGTGCTTCCAGAAATCTTCAGGTTTTATTGTTCCGTACATGTAACTCAGAAGGGCTGTGCAGGACTCTTGTGACATGTTTTCTATGTGGATTGTAGATGACTCTTTCTCATTGTCGCTGTCATGATATGAGGCCTCGAATACAGGAGAAGTTGCGGTCATAACTGCCTTATGAGCCTTAAGTGTACCATCTGCAGTTATAATGGTGAGATCAGCGTGTATAGCTTCATCAAGCATGCGAGAGAGGCATCGGAGAGTGTTTTGAGTTGCAACAGATTGCAGTTTTCCGTCAGATGGCCATATAGAACTGGCTTCTCCACCATTCAGAGGATAGATCTTCAGGTCAAGAAACTCAACATCGATAATCAAGCGACCAAGGAATGTAGTATCCACAGGCCAGACAAAGTCATCACATGTTCTAAGCACCCTTTCATGAACAGGAGAAATATAAAATCTACGGGAGGAGGAGGATGAAGAAGAAGCAGTATTCGAAACTCGAAGAATGAATCGAGCAATAGGAGGCTGCTCTTTAGAAACACGCGAATGTTCAGGAAACAGATGAATATACAAATACCTATTTCTCTCTATAGACAAGTACCAGTTCCAAATTCCGAGTTTGAAAGGGTCGGATTTCTTGTAAGAACAGGGTCCGAAGTTGTCGATTTTCCATTGAGCTAATCGTGAAATGGTTTCAACCTTGGAATCTCCCATTTTTGTTTCTTTCGCAAATTGTTAGTTAACAACACTGGCTCATGAATGAGTTAGTTCTTCACTCTTCATCCAACAAACGTTGGGCAGAGAAGCAATTGTTAGACTCTGTCTTCTTGTGGAAAAAAAGTAAAACGTCTTCATTCATCTAAACT
TARGET=959,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0057
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.005674
PRIMER_LEFT_SEQUENCE=TCATCACATGTTCTAAGCACCC
PRIMER_RIGHT_SEQUENCE=CCCTGTTCTTACAAGAAATCCG
PRIMER_LEFT=905,22
PRIMER_RIGHT=1146,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.8000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.0332
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.033137
PRIMER_LEFT_1_SEQUENCE=TCATCACATGTTCTAAGCACCC
PRIMER_RIGHT_1_SEQUENCE=CAACTTCGGACCCTGTTCTTAC
PRIMER_LEFT_1=905,22
PRIMER_RIGHT_1=1156,22
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=60.033
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.0332
PRIMER_LEFT_2_PENALTY=0.000037
PRIMER_RIGHT_2_PENALTY=0.033137
PRIMER_LEFT_2_SEQUENCE=TCATCACATGTTCTAAGCACCC
PRIMER_RIGHT_2_SEQUENCE=AACTTCGGACCCTGTTCTTACA
PRIMER_LEFT_2=905,22
PRIMER_RIGHT_2=1155,22
PRIMER_LEFT_2_TM=60.000
PRIMER_RIGHT_2_TM=60.033
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.0332
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.033137
PRIMER_LEFT_3_SEQUENCE=TCATCACATGTTCTAAGCACCC
PRIMER_RIGHT_3_SEQUENCE=ACAACTTCGGACCCTGTTCTTA
PRIMER_LEFT_3=905,22
PRIMER_RIGHT_3=1157,22
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.033
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=253
PRIMER_PAIR_PENALTY_4=0.0613
PRIMER_LEFT_4_PENALTY=0.000037
PRIMER_RIGHT_4_PENALTY=0.061230
PRIMER_LEFT_4_SEQUENCE=TCATCACATGTTCTAAGCACCC
PRIMER_RIGHT_4_SEQUENCE=CCTCCTATTGCTCGATTCATTC
PRIMER_LEFT_4=905,22
PRIMER_RIGHT_4=1020,22
PRIMER_LEFT_4_TM=60.000
PRIMER_RIGHT_4_TM=60.061
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=116
=
PRIMER_SEQUENCE_ID=SSR_03628 Ps_contig_mira-and-tgicl-ass_franssen_30589
SEQUENCE=AGTACGGGGGCCACAAACAACACCTTTCAAGGAAATATGTCTCCTCTAGCATTTCTTCTCCTTGGTGTTCTCCACCTGGTTTCACATGTAAATGGCCATGGTGGTCATGCTAGTTGGATTAATGCTCACGCTACTTTCTATGGTGGAAGCGATGCTTCCGGAACAATGGGTGGAGCTTGTGGATATGGAAATTTATATAGTCAAGGTTATGGAACAAATACAGCAGCATTAAGCACAGCATTATTCAACAATGGATTGAGTTGTGGTGCATGTTATGAAATTAAATGTGTGAATGATCCACAATGGTGTACTCCTGGATCCATTATAGTTACTGCCACTAATTTCTGTCCACCAGGTGGTTGGTGTGACCCTCCAAATCACCATTTTGATCTTTCTCAACCTATTTTTCAACACATTGCTCAATATAAAGCTGGAATTGTACCTGTTGCTTACAGAAGAGTAAGATGCAGAAGAAGAGGTGGAATAAGATTCACAATCAATGGACATTCTTACTTCAATTTAGTACTTGTGACAAATGTTGGAGGTGCTGGTGATGTACATTCTGTGGCCATAAAAGGTTCAAGAACAAGATGGCAAGCCATGTCAAGAAATTGGGGGCAAAATTGGCAAAGTAACTCTTACCTTAATGGACAAAGTCTTTCCTTTGTTGTCACCACAAGTAATGGTCATAGTGTGGTTTCATTCAATGCTGCTCCAGCTAGTTGGTCTTTTGGTCAAACCTACACCGGAAGACAGTTCAACTATTAAGTAACCTTTTAATCGATATTCTAAAAATCGAATCATCCGACGATATATAGGGTTTGATCATTTAAACCGTTCGATTCAGATTTTATTCTGAAACGAACAGTTTAACATTGTTGAACCAGATCCATAGTTTTGTTGGTTCGGTTTTTAAAATATTTAGTAATAATAATAATACTAGTATTTCAATTTTCTAAGATTATTATTACATTTAAAAATTAAAAGGACTAAGTTTTTTTTAGTTGAGTAGTGTAAATGGATTTATTTGAGTTGGGTTAGATAGAAGATGGTTTAGCCCAATTTATTTATATATTTCGATTTTATTTTTTATATGGCAAGCATTTGTGGATGATGTGCTGGCTTTATTTATGCTGTGCCTCAACAAATGAGATACTTGCCATGTTGAGAAAGATTAATCTTAAAGAGATTGTTGAATGTGCTCTTTGTACTTTAATTATTGGTATATGAATGTGAAAGATCATGCTTTAATTGTGTGAC
TARGET=926,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2306
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.121675
PRIMER_LEFT_SEQUENCE=ATCCATAGTTTTGTTGGTTCGG
PRIMER_RIGHT_SEQUENCE=GAGGCACAGCATAAATAAAGCC
PRIMER_LEFT=887,22
PRIMER_RIGHT=1141,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.122
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=255
PRIMER_PAIR_PENALTY_1=0.2640
PRIMER_LEFT_1_PENALTY=0.142283
PRIMER_RIGHT_1_PENALTY=0.121675
PRIMER_LEFT_1_SEQUENCE=CCATAGTTTTGTTGGTTCGGTT
PRIMER_RIGHT_1_SEQUENCE=GAGGCACAGCATAAATAAAGCC
PRIMER_LEFT_1=889,22
PRIMER_RIGHT_1=1141,22
PRIMER_LEFT_1_TM=60.142
PRIMER_RIGHT_1_TM=60.122
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.3876
PRIMER_LEFT_2_PENALTY=0.108929
PRIMER_RIGHT_2_PENALTY=0.278625
PRIMER_LEFT_2_SEQUENCE=ATCCATAGTTTTGTTGGTTCGG
PRIMER_RIGHT_2_SEQUENCE=CAGCATAAATAAAGCCAGCACA
PRIMER_LEFT_2=887,22
PRIMER_RIGHT_2=1135,22
PRIMER_LEFT_2_TM=60.109
PRIMER_RIGHT_2_TM=60.279
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.3876
PRIMER_LEFT_3_PENALTY=0.108929
PRIMER_RIGHT_3_PENALTY=0.278625
PRIMER_LEFT_3_SEQUENCE=ATCCATAGTTTTGTTGGTTCGG
PRIMER_RIGHT_3_SEQUENCE=CACAGCATAAATAAAGCCAGCA
PRIMER_LEFT_3=887,22
PRIMER_RIGHT_3=1137,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=60.279
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=0.4209
PRIMER_LEFT_4_PENALTY=0.142283
PRIMER_RIGHT_4_PENALTY=0.278625
PRIMER_LEFT_4_SEQUENCE=CCATAGTTTTGTTGGTTCGGTT
PRIMER_RIGHT_4_SEQUENCE=CAGCATAAATAAAGCCAGCACA
PRIMER_LEFT_4=889,22
PRIMER_RIGHT_4=1135,22
PRIMER_LEFT_4_TM=60.142
PRIMER_RIGHT_4_TM=60.279
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_03629 Ps_contig_mira-and-tgicl-ass_franssen_30591
SEQUENCE=ATGAGACTAGAAAACCAAACCCTGGCTCTGTTCTTCGTTGTGCGTTACCGCTGAAACAAAGTCAACAACACTCGTTTGTTTATGCTCTTCAAAATCAACTCAGCTCAACTCAACGCAACCCTAAAACCGAATCAATGTCGTCTTCAGAGATTCTCCAACAACTTGAATCTCTGAAGGAAGAAAAGTCCAATATCGACCACAAAATATCACTTCTAGAAGCTCAATTGAAGGAAATTAATCTCCAAAACGGCGCTGCTGCATCATCATCATCATCCAATGGCTCTTTACGGTACGCTACAAATGGATTAGAACCTCACATGATTCATAGATACAGTCGCCACCTTGTTCTTCCTTCCTTTGGTGTTCAAGGACAAGCAAATTTGTTGAAGTCGTCGATTTTAGTTGTGGGAGCTGGAGGGCTTGGTGCTTCTGCATTGTTATATTTTGCAGCTGCTGGTGTTGGTAAACTGGGTGTTCTTGATCATGACAAGGTTGAGTTGAATAATATGCATAGACAGATTATACACACGGAAGCATATATTGGTCAGCCAAAAGTTAAATCCGCTGCTGCTGCTTGTCGCTCGATCAATTCATCTATTGAAGTCGTGGAACATGAAGAAGCTTTGAAGAATTCCAATGCTTTGGAAATTTTCAGCAAATACGATATAATAGTGGATGCAACAGATAATGCTCCCACCCGGTATTTGATCAGTGACTGCTGCGTGGTACTAGGAAAGCCCCTTGTATCAGGTGCGGCCGTGGGATTGGAAGGACAGCTCACTATCTACAATCACAAAGGTGGTCCTTGCTTTCGATGCCTCTTTCCAACCCCACCACCTAGAGCAGCATGCCAAAGTTGCGCCGATGGTGGAGTACTAGGAGTAGTTCCTGGTATAATTGGCTGTCTCCAAGCTCTCGAGGCGATTAAGATTGCGGCTTCTGTTGGTGAACCACTCTCTGGAAGGATGCTTCTCTTTGATGCATTGGCTGCACGAATCCGTATTGTTAAAATCAGAGGAAGGTCTTTGCAATGTGAAGCTTGTGGAGAAAATGCAATAATGAATAAGCAGTACTTCCGAGAATTTGATTATGAGAAGTTCACTCAAACTCCATTGTGTGAGGCTCCGTTGAAGTTAAATCTACTTCCTAGTGACTCGCGAATCAGCAGCAAGGAGTACAACGAAATCATTCTCAACAAGGAACCACATGTGCTTGTCGATGTTAGACCAGCTCACCATTTCAAGATCGTGTCTCTTCCGAATTCGGTCAACATTCCATTCGCAGATATAGAGTTTAGGTTGCCTGAAATATCTTCAATTTTGAAGAAAGACAAAGAGGAAAAAAGTGCGGAATTGTATGTTGTTTGCAGAAGGGGAAATGATTCTCAAAGGGCTGTTCAATACCTTCACAAAATGGGTTTGGTTCTGCTAAGGATATTGTTGGAGGTTTGGAGTCTTGGGCTAAAAATGTTGATCCTAACTTCCCCACCTATTAGCTATCTAGTTAAACTTTGATCAAGTTATACTTCTAAGTTGAAATTATTATATTATTTTATTAGGGGTGCTGTTTTCATTTTCCATGTTATTTTAGAATTCCTTTGANAACATTTTGCATTGGTTAGAGAATATTTT
TARGET=259,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0227
PRIMER_LEFT_PENALTY=0.010545
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TGTCGTCTTCAGAGATTCTCCA
PRIMER_RIGHT_SEQUENCE=ACACCAAAGGAAGGAAGAACAA
PRIMER_LEFT=135,22
PRIMER_RIGHT=363,22
PRIMER_LEFT_TM=59.989
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.0227
PRIMER_LEFT_1_PENALTY=0.010545
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=TGTCGTCTTCAGAGATTCTCCA
PRIMER_RIGHT_1_SEQUENCE=AACACCAAAGGAAGGAAGAACA
PRIMER_LEFT_1=135,22
PRIMER_RIGHT_1=364,22
PRIMER_LEFT_1_TM=59.989
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.1368
PRIMER_LEFT_2_PENALTY=0.124655
PRIMER_RIGHT_2_PENALTY=0.012153
PRIMER_LEFT_2_SEQUENCE=CGAATCAATGTCGTCTTCAGAG
PRIMER_RIGHT_2_SEQUENCE=ACACCAAAGGAAGGAAGAACAA
PRIMER_LEFT_2=127,22
PRIMER_RIGHT_2=363,22
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=0.1368
PRIMER_LEFT_3_PENALTY=0.124655
PRIMER_RIGHT_3_PENALTY=0.012153
PRIMER_LEFT_3_SEQUENCE=CGAATCAATGTCGTCTTCAGAG
PRIMER_RIGHT_3_SEQUENCE=AACACCAAAGGAAGGAAGAACA
PRIMER_LEFT_3=127,22
PRIMER_RIGHT_3=364,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.1474
PRIMER_LEFT_4_PENALTY=0.010545
PRIMER_RIGHT_4_PENALTY=0.136881
PRIMER_LEFT_4_SEQUENCE=TGTCGTCTTCAGAGATTCTCCA
PRIMER_RIGHT_4_SEQUENCE=CACCAAAGGAAGGAAGAACAAG
PRIMER_LEFT_4=135,22
PRIMER_RIGHT_4=362,22
PRIMER_LEFT_4_TM=59.989
PRIMER_RIGHT_4_TM=60.137
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_03630 Ps_contig_mira-and-tgicl-ass_franssen_30591
SEQUENCE=ATGAGACTAGAAAACCAAACCCTGGCTCTGTTCTTCGTTGTGCGTTACCGCTGAAACAAAGTCAACAACACTCGTTTGTTTATGCTCTTCAAAATCAACTCAGCTCAACTCAACGCAACCCTAAAACCGAATCAATGTCGTCTTCAGAGATTCTCCAACAACTTGAATCTCTGAAGGAAGAAAAGTCCAATATCGACCACAAAATATCACTTCTAGAAGCTCAATTGAAGGAAATTAATCTCCAAAACGGCGCTGCTGCATCATCATCATCATCCAATGGCTCTTTACGGTACGCTACAAATGGATTAGAACCTCACATGATTCATAGATACAGTCGCCACCTTGTTCTTCCTTCCTTTGGTGTTCAAGGACAAGCAAATTTGTTGAAGTCGTCGATTTTAGTTGTGGGAGCTGGAGGGCTTGGTGCTTCTGCATTGTTATATTTTGCAGCTGCTGGTGTTGGTAAACTGGGTGTTCTTGATCATGACAAGGTTGAGTTGAATAATATGCATAGACAGATTATACACACGGAAGCATATATTGGTCAGCCAAAAGTTAAATCCGCTGCTGCTGCTTGTCGCTCGATCAATTCATCTATTGAAGTCGTGGAACATGAAGAAGCTTTGAAGAATTCCAATGCTTTGGAAATTTTCAGCAAATACGATATAATAGTGGATGCAACAGATAATGCTCCCACCCGGTATTTGATCAGTGACTGCTGCGTGGTACTAGGAAAGCCCCTTGTATCAGGTGCGGCCGTGGGATTGGAAGGACAGCTCACTATCTACAATCACAAAGGTGGTCCTTGCTTTCGATGCCTCTTTCCAACCCCACCACCTAGAGCAGCATGCCAAAGTTGCGCCGATGGTGGAGTACTAGGAGTAGTTCCTGGTATAATTGGCTGTCTCCAAGCTCTCGAGGCGATTAAGATTGCGGCTTCTGTTGGTGAACCACTCTCTGGAAGGATGCTTCTCTTTGATGCATTGGCTGCACGAATCCGTATTGTTAAAATCAGAGGAAGGTCTTTGCAATGTGAAGCTTGTGGAGAAAATGCAATAATGAATAAGCAGTACTTCCGAGAATTTGATTATGAGAAGTTCACTCAAACTCCATTGTGTGAGGCTCCGTTGAAGTTAAATCTACTTCCTAGTGACTCGCGAATCAGCAGCAAGGAGTACAACGAAATCATTCTCAACAAGGAACCACATGTGCTTGTCGATGTTAGACCAGCTCACCATTTCAAGATCGTGTCTCTTCCGAATTCGGTCAACATTCCATTCGCAGATATAGAGTTTAGGTTGCCTGAAATATCTTCAATTTTGAAGAAAGACAAAGAGGAAAAAAGTGCGGAATTGTATGTTGTTTGCAGAAGGGGAAATGATTCTCAAAGGGCTGTTCAATACCTTCACAAAATGGGTTTGGTTCTGCTAAGGATATTGTTGGAGGTTTGGAGTCTTGGGCTAAAAATGTTGATCCTAACTTCCCCACCTATTAGCTATCTAGTTAAACTTTGATCAAGTTATACTTCTAAGTTGAAATTATTATATTATTTTATTAGGGGTGCTGTTTTCATTTTCCATGTTATTTTAGAATTCCTTTGANAACATTTTGCATTGGTTAGAGAATATTTT
TARGET=564,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3220
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.309858
PRIMER_LEFT_SEQUENCE=TGTTCTTCCTTCCTTTGGTGTT
PRIMER_RIGHT_SEQUENCE=AGCTTCTTCATGTTCCACGACT
PRIMER_LEFT=343,22
PRIMER_RIGHT=622,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.310
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.3220
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.309858
PRIMER_LEFT_1_SEQUENCE=TTGTTCTTCCTTCCTTTGGTGT
PRIMER_RIGHT_1_SEQUENCE=AGCTTCTTCATGTTCCACGACT
PRIMER_LEFT_1=342,22
PRIMER_RIGHT_1=622,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=60.310
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.3707
PRIMER_LEFT_2_PENALTY=0.060824
PRIMER_RIGHT_2_PENALTY=0.309858
PRIMER_LEFT_2_SEQUENCE=ATACAGTCGCCACCTTGTTCTT
PRIMER_RIGHT_2_SEQUENCE=AGCTTCTTCATGTTCCACGACT
PRIMER_LEFT_2=328,22
PRIMER_RIGHT_2=622,22
PRIMER_LEFT_2_TM=60.061
PRIMER_RIGHT_2_TM=60.310
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.4467
PRIMER_LEFT_3_PENALTY=0.136881
PRIMER_RIGHT_3_PENALTY=0.309858
PRIMER_LEFT_3_SEQUENCE=CACCTTGTTCTTCCTTCCTTTG
PRIMER_RIGHT_3_SEQUENCE=AGCTTCTTCATGTTCCACGACT
PRIMER_LEFT_3=338,22
PRIMER_RIGHT_3=622,22
PRIMER_LEFT_3_TM=60.137
PRIMER_RIGHT_3_TM=60.310
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.4467
PRIMER_LEFT_4_PENALTY=0.136881
PRIMER_RIGHT_4_PENALTY=0.309858
PRIMER_LEFT_4_SEQUENCE=CTTGTTCTTCCTTCCTTTGGTG
PRIMER_RIGHT_4_SEQUENCE=AGCTTCTTCATGTTCCACGACT
PRIMER_LEFT_4=341,22
PRIMER_RIGHT_4=622,22
PRIMER_LEFT_4_TM=60.137
PRIMER_RIGHT_4_TM=60.310
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_03631 Ps_contig_mira-and-tgicl-ass_franssen_30592
SEQUENCE=AAACACGCGCACTCTCTCTCTCTCTCTCTTTCTCTCACTTCACGATGGAGAAAGCAGAGAGCTCCACCGCCTCCGCCTCCGCCTCCACCTCCGACCAAGACTCCGACGAAAAGCACCGCACACAACACCACCTAACAGTTCCATCAGGTCTAACTCAACACGAGTTTGTCTCACTCATCTCTTTCGTCGCTGAACACCACACCTACCTCATAGGTCCCGGCCAATGCTCCACCTTGCTCGCTCAACGAATCCACGCGCCTCCTGAAACCGTCTGGTCCGTCGTACGCAGCTTCGACAAACCACAAATCTACAAACACTTCATCAAAAGCTGCTCTCTCAAAGAAGGTTTTCAAATGAAAGTCGGTTGCACCAGAGACGTCAATGTCATCTCCGGTCTCCCGGCGGCAACTAGCACCGAGAGACTCGACGTTCTCGATGACGAACGCCGTGTCACCGGATTCAGTATCACCGGCGGCGAACACCGCTTGAGAAACTACCGTTCTGTTACCTCCGTCCACGGTTTTGATGACGGTGACGGCGAGATCCGGACGGTTGTTCTGGAATCTTATATAGTTGATGTTCCTGAAGGTAACACCGAAGAGGATACGCGTCTTTTTGCTGATACCGTTGTGAAGCTTAATCTTCAGAAGCTTGCGTCTGTCACCGAAGGAAAGAACCGTAACGGTGACGGTAAGTCACATTAGTAGGGACCACATCACCGCAAGTTTTATTAAATACCGAAAGAAAAAAAAGGATTGGAAATTGAAATAGAAAAAAATTTGTTGTAACTGTTTTGAAAGTGTTGTGAAAAGTTGAAACTATCGCCGGCTGGCTGGCTCGCTCCCTCATGAATACNT
TARGET=12,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03632 Ps_contig_mira-and-tgicl-ass_franssen_30592
SEQUENCE=AAACACGCGCACTCTCTCTCTCTCTCTCTTTCTCTCACTTCACGATGGAGAAAGCAGAGAGCTCCACCGCCTCCGCCTCCGCCTCCACCTCCGACCAAGACTCCGACGAAAAGCACCGCACACAACACCACCTAACAGTTCCATCAGGTCTAACTCAACACGAGTTTGTCTCACTCATCTCTTTCGTCGCTGAACACCACACCTACCTCATAGGTCCCGGCCAATGCTCCACCTTGCTCGCTCAACGAATCCACGCGCCTCCTGAAACCGTCTGGTCCGTCGTACGCAGCTTCGACAAACCACAAATCTACAAACACTTCATCAAAAGCTGCTCTCTCAAAGAAGGTTTTCAAATGAAAGTCGGTTGCACCAGAGACGTCAATGTCATCTCCGGTCTCCCGGCGGCAACTAGCACCGAGAGACTCGACGTTCTCGATGACGAACGCCGTGTCACCGGATTCAGTATCACCGGCGGCGAACACCGCTTGAGAAACTACCGTTCTGTTACCTCCGTCCACGGTTTTGATGACGGTGACGGCGAGATCCGGACGGTTGTTCTGGAATCTTATATAGTTGATGTTCCTGAAGGTAACACCGAAGAGGATACGCGTCTTTTTGCTGATACCGTTGTGAAGCTTAATCTTCAGAAGCTTGCGTCTGTCACCGAAGGAAAGAACCGTAACGGTGACGGTAAGTCACATTAGTAGGGACCACATCACCGCAAGTTTTATTAAATACCGAAAGAAAAAAAAGGATTGGAAATTGAAATAGAAAAAAATTTGTTGTAACTGTTTTGAAAGTGTTGTGAAAAGTTGAAACTATCGCCGGCTGGCTGGCTCGCTCCCTCATGAATACNT
TARGET=827,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03633 Ps_contig_mira-and-tgicl-ass_franssen_30593
SEQUENCE=TATAAACTGATTTCGGGTCACCAACATTGGTCACAGTCCTGATGAAATGTGTAGACNTTTTATTGTTTTCCATATTGTTGAAAAACTGCAGATAATGATGGATAGTTCAAGTTTCCGGCGTGTCCGGCTTTTTTAGCGTCGCTACAATCTGCAGTTTTTCTTGTGATTGTTTGGATATTCTTTGTGGTGTAGTTTGAGTTACACAAGAAATCGACATAATCGTAAACTGAGATATCGTAAACCAAACCAGGATCCAATGCTTTCTCGGGATGAACATGTCCTGCTCCATAATCAAACACGGATGAAACGTTCCCGTTTGATTCATCCAACATTGGATCGCCTTTGTTATCGACCGTATAAGCAGTGGTCATAAGAGCTGATTTGATAGCAGCTGGACTCCAATCAGGGTGAGCTGCTTTCAATAAAGCAGCTAAACCGGAAACGTGAGGACAAGCCATAGATGTACCCGACAATATGTTAAATTCAGTCCTTCTATGATCAGAAGCTATACCAGAAGGTCCTAACTTTAGTACTGGCCATGCGGCTAGAATGTTCAAACCAGGTGCTATAACATCAGGTTTCAAAATCTCGGGCGATTCAGGGTTAGTTCCCTTAAATACAATAGTTGCGGTCGGTACTGATCCAACCGATTTATCTGCGGAGGCAATGTAACTTCTAATTACATCGCYRCCTNTCGNACCAACCGATGCCGCCGACGAACCCTTATCCACTACCTCTTTAAACTCACCGTCTTCATCCCTCCACGCGTCTTCCTCTCCACTTCAACCAAACTCCGCGCTCACCCATGACTCTTCGTCCACCGATCTGACGCGTTCACCACCATAAACTTTCGGCGCACACTCCATCCTCCACCATCATCTTCTCCCTCAGTTAATCGCCGGATTCAAACCGCACCTCCAGTTCCTTCCTCTGCTTCAACCCTCAATCGCTAACAACACAAACCCGGCTCCATCTTTCTCCTCCATCGGTCACCTTCAATCGCTCAACAACCTTCCGGCATCATCACCGCCCAAACACCAAAATCTTCGTTGTGGCATAATCATTTTTTCCTCCATTTTTCAGAACCAAACCCTTGACACTCTATCTCCGTAAATCCAAAATTTACCTTTGCATCTGTTGGTGTGTGAGGAACCGGTGAGCGAGCGAGCTAGCGCCGAAAAATGGAAGATGCTGGAACAAAAGAAAGAACAGAAGAAACAAATCTTACAAAATCAAACGTGGAAGTAACTGAATCCAAGATTCGTAAGCAAGCCTCCAACGAACCATGAATAACAACATTACAGTAACGACAATTCAATTTCTTGTGGAGCTCTGTATATTGATGTTGTGTGGTTTAACTTCCAAAGTTGAATAAAGTTTTTAGAAACTTTTTAATTTATTCAGTGTTATTCTTGCTTTAAGAAAAGAAGAGTGATATGTTAAATGTTTGATAACATTGGTAGTGTATGAAGTCCATGCCATTGTTTACTATGCATGCTTACTAAATGTGTGACGATTTGGTCTAAAAAATGAAAAACATAGAAAAAGCAAACCAAAGGTAGAGAGGTTTGGACGGTTCACCGTTTTTAAGAATGACAAATTAGTTTTTCTTTCTTCCTGCTGTATTTTCTTGATTTTCTTTCACCAAGGAAGGTGTGGTGGAAGGTTGAAAAGTCAA
TARGET=1158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03634 Ps_contig_mira-and-tgicl-ass_franssen_30595
SEQUENCE=GACTATTACTAACATTGAATCATTTGTTTATGGTGATGACCATACAAAGATATTTATACATTAAAAAATACACAAAACAAATCCAAAACAAACTTAAATTACTAGAAATAATTAAATTAAATTAAATTATCTAAAGATGAATCAATTCTTTTTTCCTAACAATCTCTTGAAGCTTGTTGCAGAAATCATCAATATAAGATGATTCAAGATCTTCATTAAGCAACATCTCTCTAATCTTAGCATGATTAACCCTCACTTTCTTACTGGTTTCATTCTCATCCTCCATCACAATACTCACAGCTTTACAAACACTATCCTTAGTATACAAACCATCTTCACCTTTCTCGACTTCTACACCAACTTTCAAGTTATTTCCCATCATCCTAGCATTCAATATTTGGTCACCATAATTTGGCAGCAAAACCAATTGACATTTATTCACCAATGCCTCAGATAAAGAACCCGAACCACAATGTGTAATGAAACATCCAACAGAAGGATGTTCCAAAATCAGTTGCTGCTGTACCCAACCACCATAAACAACACCTCTTCCTTTCGTTCTTTCTGCAAACCCTTCTGGTAATGCTTCTTCCAATGTTGCAAAACCAAAAGGTGGCTTCAAAGCTGCAAAAAATGGTATACCGGTTAACTCAAGACCGAGCATTAATTCTTGAAATAGATTTGGTCTTAAAACACATTCACTTCCAAAACAACAATAAACAACTGAATCCGTCTTGAATCCAGCAAGCCAAGTAGACCAATTTTCATCTAAAACAGAATTTGGTTTTTCAAGTATAACAGGTCCTGAAGTTAGAACCGGTTTGTTAAATTGTTTATGTATGTAATCAAGATAAGGCCCTTCAATTTCTTTGCATGTTCTGAATCCCAATGCATCGGCTTCATTCAAAGAAATGGCTTGTCTATCATAGAAAAGCACATTGCTACCGAAAATTTCTTTCCTTTTAGCAACAAAGATTTTAGCTTCATGATTATAAAGCTTGATTGATGAATCAGGGTAACCAGAAGGAGGTTCCATGAGATCAAATTCTGTTAAACTTGTTCCCTTTAAATATCTAGCTGGTGTTAGAGTATAACCAACCATAACCGAACTAGCAGTGCAATAATGAATAGCTTTGATGCCTAAACGTTTGGTTAAAGATGGAATCCAATGCGTGAAATCGTAGAAAACAATGTGAGGTTTGAGATTAGTGAGATGAGTTTGGATGTCGGGTTGTGTTAGATCCATGGCTGTCATGATGTGTGGTTGTAAAGGGAAAGGGACATCAGCTGTTGTTTCTGCATTGGGAGGAAGACCTTCAACATGAGGAACTGTGATAGTGACAAAAGTGATTGATTGAGGGTGGAGATTGAATGGTTGTAATTTAGATCGTGCTGATTTAGGTGTGAAAAAAGTGACGGTGTGACCTTTTTTTGCTAACTTGTTAGCTAGGTGAATGAATGCAGTTTGATGGCCCATGGCAAACCATGGATACATTGCTATGTGTAAAGAAGATGACGAATCCATAGTGATAGAAAACAAAATATCTTGAACAATATTGTAAACGATTTTGGTGGTCGNCCCGCGTA
TARGET=110,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03635 Ps_contig_mira-and-tgicl-ass_franssen_30596
SEQUENCE=TCGTTATTTATAGGAGTATATATGGCTCAATAAAAATATTAATTAAAAAATCGAACCAACCGAATCAACCCAAACCTCATTGATTGATTTGGTTTGGTTTGGTTTTTATTTCTAAAAGTCAATCGAATCAAACTGAACCGCATGTTTTTTTTTCTTCTTACGGTTCGGGTGTTTTATATCGCAAAAAACCGCTCAAACCGCACCGCGAACATCCCTAGTGATGACCATACAAACAAATATTCATTAGGTAAAAAGAAACACAAGACAAATCCAAAACAAATCCAGTGCCACACAAACTTAAATTACTAGAAATAATTAAATTAAACTGCATAATGTTGAATCAATTCTTTTCCGTAACAATCTCTTGAAGCTTGTTGCAGAAATCATCAATATAAGATGATTCAAGATCTTCATTAAGCAACATCTCTCTAATCTTAGCATGATTAACCCTCACTTTCTTACTGGTTTCATTCTCATCATCCATCACAATACTCACAGCTTTACAAACACTATCCTTAGTATATAAACCATCTTCACCTTTCTCGACTTCTACACCAACTTTCAAGTTATTTCCCATCATCCTAGCATTCATTATCTGATCACCAATGATTGGCAACAAAACCAATTGACATTTATTCACCAATGCCTCAGACAAAGAACCTGAACCACAATGTGTAATGAAACACCCAACAGAAGGATGCTCCAAAATCAGTTGCTGCTGTACCCAACCACCAAAAACAACACCTCTTCCTTTTGTTCTTTCTGCAAACCCTTCTGGTAATGCTTCTTCCACTGTTGCAAAACCAGAAGGTGGCTTCAAAGCCGCAAAAAATGGCATACCTGTTAACTCAAGTCCAAGCATTAATGCTTGAAATAGATTCACTTCCAAAACACATTCACTTCCCAAACAACAGTAAACAACTGAATCTGTCTTGAATCCAGCAAGCCAAATAGACCAATTTTCATCCAAAACAGAATTAGGTTTTTCGAGTATAACAGGTCCTGAAGATAGAACCGGTTTATTATACTGTTTATGTAAGTAATCAAGATAAGGTCCTTCGATTTCTCTACAAGTTCTGAATCCTAATGCATCAGCTTCATTGAAAGCAATGACTTGTCTATCATAGAAAGAAACATTACTACCAAAAGTTCTGATTCCCAATGATATCAAAAGTTTTGCTTCATCACTATGAAGCTTGATAGATGAATCAGGGTAACCAGGAGGAGGTTCCATGAGATTAAATTCTGTTATATTTTGATTTAAAAATCTAGCTGGTACTAAGTTGTAGCCAATCATAACCGAACTACCAGTGCAATAAAGAAGAGATTTGATACCTAAACGTTTTGTTAAAGATGGCATCCAATGTGTGAAATCATAGAAAACAAAGTGAGGTTTGAGATTAGTGAGATGAGTTTCAATGTGGGGTTGTGTTAGATCCATGGCTGTCATGATGTGTGGTTGTAAAGGGAATGAGACATCAGCTGTTGTTTCTGCATTGGGAGGAAGGCCTTCAACATGGGGAACTGTGATAGTGACGAAAGTGATTGATTGAGGGTGGAGATTGAATGGTTGTAATTTAGATTGTACTGATTTAGGTGTGAAAAATGTTATTTTGTGGCCTTTATTGGCTAGTTTGTTAGCTAAGTGAAGAAATGGAGTTTGGTGGCCCATGGCAAACCATGGGTACATTGCTATGTGTAAAGAAGATGACGAATCCATAGTGATAGAAAACAAAATATCTTGAACAATATAGCAAACAATTTTGGTGGTC
TARGET=88,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4399
PRIMER_LEFT_PENALTY=1.212650
PRIMER_RIGHT_PENALTY=0.227281
PRIMER_LEFT_SEQUENCE=CAACCCAAACCTCATTGATTG
PRIMER_RIGHT_SEQUENCE=ATAAAACACCCGAACCGTAAGA
PRIMER_LEFT=65,21
PRIMER_RIGHT=176,22
PRIMER_LEFT_TM=60.213
PRIMER_RIGHT_TM=59.773
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=112
PRIMER_PAIR_PENALTY_1=1.6731
PRIMER_LEFT_1_PENALTY=1.212650
PRIMER_RIGHT_1_PENALTY=0.460467
PRIMER_LEFT_1_SEQUENCE=CAACCCAAACCTCATTGATTG
PRIMER_RIGHT_1_SEQUENCE=TAAGTTTGTGTGGCACTGGATT
PRIMER_LEFT_1=65,21
PRIMER_RIGHT_1=299,22
PRIMER_LEFT_1_TM=60.213
PRIMER_RIGHT_1_TM=59.540
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=1.6731
PRIMER_LEFT_2_PENALTY=1.212650
PRIMER_RIGHT_2_PENALTY=0.460467
PRIMER_LEFT_2_SEQUENCE=CAACCCAAACCTCATTGATTG
PRIMER_RIGHT_2_SEQUENCE=TTAAGTTTGTGTGGCACTGGAT
PRIMER_LEFT_2=65,21
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=60.213
PRIMER_RIGHT_2_TM=59.540
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=1.7787
PRIMER_LEFT_3_PENALTY=1.212650
PRIMER_RIGHT_3_PENALTY=0.566078
PRIMER_LEFT_3_SEQUENCE=CAACCCAAACCTCATTGATTG
PRIMER_RIGHT_3_SEQUENCE=TTTAAGTTTGTGTGGCACTGGA
PRIMER_LEFT_3=65,21
PRIMER_RIGHT_3=301,22
PRIMER_LEFT_3_TM=60.213
PRIMER_RIGHT_3_TM=60.566
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=1.9333
PRIMER_LEFT_4_PENALTY=1.212650
PRIMER_RIGHT_4_PENALTY=0.720663
PRIMER_LEFT_4_SEQUENCE=CAACCCAAACCTCATTGATTG
PRIMER_RIGHT_4_SEQUENCE=TAAAACACCCGAACCGTAAGAA
PRIMER_LEFT_4=65,21
PRIMER_RIGHT_4=175,22
PRIMER_LEFT_4_TM=60.213
PRIMER_RIGHT_4_TM=60.721
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=111
=
PRIMER_SEQUENCE_ID=SSR_03636 Ps_contig_mira-and-tgicl-ass_franssen_30599
SEQUENCE=AAANAATTTGCCAAAAATAACCACCATGTTCAATTGCATTTCAAAGGAGAGCAGTGTCAACGCATCGAACAAACGATGGGAAATAACTTCGTAACAACTCACATTATCTGAATAAAGAAACAAACTATCAAACTACAATCCAGCAGCTGTCTGAAACTAGGAGTTCCTACATAATCAAGAACTATCTCCCCTAATTCTTCCTCCTCATTTGAATATCTAACTCAAAAGATCAACATGAACTATTAAAAATTAGTAACGGTCAAATGATGATGGCCGAGCAGTCCTGCTGGGGCGGTCATAAGGAGCAGGCCTACCCCTATAACTTCTTCCTTCCTCACGAGCTGGTATTCCACTTCCATTTCTCTCAGCACCTCCACGAGCACCACCATAGCGGTCATAGCCTCTTTCTTTCCCATAACCATTTTGCGGGTAGTCTGAGCCACTGCCATATCGATCAGCAAATCGATCTCCTCCACTTGTTGCATACCTGTCACTAGCATAGCGATCACGGCCACTATATTTGTAGTCTCGGTTGTCTAAGCGGTCCCTGTCCCCGTAACGTCCTCCATCATAACGATCATCGATGTACCGCTCACGTTCACCAAAGCGATCCCCGTGTCCACCAGCACCTCCAAACCTAGGACGTGAAGAAAAAGAACCACTACCCCGACCACGACCACCATCTCCTCCTGCCAAAGGACAATCTCGGGCCCAGTGTCCTGGACGACCACATTTGAAACAGTCATCCTGTCCTACCCTATCTCCAGCCCCGTAGCTACCCCTGCCACCTGAAGAGTAGCCACCTCTGTACCCTTGGTCTGCATCATCACCCCCTGCCCTAGGCTGTGCCTTGTTGACTGAGATGATGCGATCGCCGATTTCCCGTCCATTCATTTCTTTAATAGCATCCTCCATTCCCCGACGGTCAGAAAATGTAATAAACCCAAATCCACGAGGACGTCCTGTATCCCTTTCCATCATGATCTGACATTCGAGAATCTTGCCGTAACGATCAAAGGCATGTTCTAGCTGACGTTCGGTAACGTCCCAACCCAATCCACCAACAAAGATTCGATTATCTTCCTTTCCAGACATCAGTGATTCTCAATTATCGCAAACACGTTGAATCCTGAAACAAATTATGAATGAATGAAGAGAGAAATGCTATAGAAGTTGAAAATTGAGATGCTAATCGAATGAGTAATTTTTGGGGAAATTAGGGTTTTGAAGGAAACCCTTGAGGTTGAAGGAGTGTGAAGATTCCAAATCCAACGCTCTTNTTAGT
TARGET=1142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1748
PRIMER_LEFT_PENALTY=0.058853
PRIMER_RIGHT_PENALTY=0.115965
PRIMER_LEFT_SEQUENCE=TGTTCTAGCTGACGTTCGGTAA
PRIMER_RIGHT_SEQUENCE=TCTTCACACTCCTTCAACCTCA
PRIMER_LEFT=1021,22
PRIMER_RIGHT=1259,22
PRIMER_LEFT_TM=59.941
PRIMER_RIGHT_TM=59.884
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.2620
PRIMER_LEFT_1_PENALTY=0.058853
PRIMER_RIGHT_1_PENALTY=0.203172
PRIMER_LEFT_1_SEQUENCE=TGTTCTAGCTGACGTTCGGTAA
PRIMER_RIGHT_1_SEQUENCE=CTAANAAGAGCGTTGGATTTGG
PRIMER_LEFT_1=1021,22
PRIMER_RIGHT_1=1283,22
PRIMER_LEFT_1_TM=59.941
PRIMER_RIGHT_1_TM=59.797
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.2634
PRIMER_LEFT_2_PENALTY=0.058853
PRIMER_RIGHT_2_PENALTY=0.204533
PRIMER_LEFT_2_SEQUENCE=TGTTCTAGCTGACGTTCGGTAA
PRIMER_RIGHT_2_SEQUENCE=AGGGTTTCCTTCAAAACCCTAA
PRIMER_LEFT_2=1021,22
PRIMER_RIGHT_2=1237,22
PRIMER_LEFT_2_TM=59.941
PRIMER_RIGHT_2_TM=60.205
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=217
PRIMER_PAIR_PENALTY_3=0.3477
PRIMER_LEFT_3_PENALTY=0.058853
PRIMER_RIGHT_3_PENALTY=0.288804
PRIMER_LEFT_3_SEQUENCE=TGTTCTAGCTGACGTTCGGTAA
PRIMER_RIGHT_3_SEQUENCE=TTGGAATCTTCACACTCCTTCA
PRIMER_LEFT_3=1021,22
PRIMER_RIGHT_3=1265,22
PRIMER_LEFT_3_TM=59.941
PRIMER_RIGHT_3_TM=59.711
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.3477
PRIMER_LEFT_4_PENALTY=0.058853
PRIMER_RIGHT_4_PENALTY=0.288804
PRIMER_LEFT_4_SEQUENCE=TGTTCTAGCTGACGTTCGGTAA
PRIMER_RIGHT_4_SEQUENCE=TGGAATCTTCACACTCCTTCAA
PRIMER_LEFT_4=1021,22
PRIMER_RIGHT_4=1264,22
PRIMER_LEFT_4_TM=59.941
PRIMER_RIGHT_4_TM=59.711
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=244
=
PRIMER_SEQUENCE_ID=SSR_03637 Ps_contig_mira-and-tgicl-ass_franssen_30602
SEQUENCE=TAGAAATTNTTGGTTTGTTAATATTTTTGTCTGTCTACAAGTTTCGTCATTAACAACTTCACTCCTTTTCAATATTAGAGATAACTTCTTCTTCTTCTTCTCTCCCTCTTTCATTCATTCAACAAATAACAATGTCTCATTCTTCTTTTCTCACTCCCTCTTCTTCTCTTACCTTCAATTCCCCTCTTCTTTCCCCTCTCCCTTCTTCTTCTTCTCCCCTCTCTCACAGTTCTTCTCTTCTCTCCGGGAGATTGAGTAAATCTGTAGGATTTAGAAAGACTACTCTAAAATCTCCGGTGAAAGCTGTGTATGGTGGTGAATTTCGGGGACCTGAGAGGAGTTTTCGTCAGGGTATTTGGTCCATAAGGGAGGATTTGGGAGTGCCGTCTTCGCCTTATTTGCCTGTCTATGCTCAAGGACAAGGGCCGCCTCCAATGGTTCAGGAGCGTTTCCAGAGTGTTATAAGCCAGCTTTTCCAATATAGAATAATCCGTTGTGGTGGAGCAGTTGATGATGATATGGCAAATATCATAGTTTCTCAGCTCCTTTATCTTGATGCTGTTGATCCAAATAAGGATATTGTCATGTATATAAACTCTCCGGGAGGATCGGTTACAGCTGGTATGGCAATATTTGATACAATGAGGCATATCAGACCTAATGTGTCCACTGTCTGTGTTGGATTGGCGGCTAGTATGGGAGCTTTTCTGCTTAGCGCAGGGACCAAAGGAAAAAGATTCAGCTTGCCAAATTCAAGGATAATGATTCATCAACCGCTTGGTGGTTTTCAAGGAGGGCAATCTGACATAGATATTCAGGCAAATGAAATGCTTCATCACAAGGCAAACCTGAATGGATATCTGTCCTATCATACTGGTCAAAGTTATGACAAAATTAACCAGGATACTGACCGCGATTTTTTCATGAGCGCAAAAGAAGCTAAAGAATATGGACTCATTGATGGTATCATTATGAATCCTCTCAAAGCTTTTCAGCCTTTGGCTGCTGCAGAAGAGGGTGCAGATCGAGCTATTGCCTGACCTCGATCCAAGGTATAAAAAAACAAGAATGTTGTTTTTGGTTGGCTGCGCTTAGTATAGCTGAATTTTTCACAGATGTCGATATCTGTATAATGGTGTACTGAGAAATGAAGCCAAAGTGATACTTAGTGTAATGTATTGCCTCAAAGTTCATACTTTTAGAATCTTCCTTAAGGGATCCTGGATTCACATTTTTCCTTCACAGGCATTCTATTTTCTTGTGATAACGGATGTCTGGTGTGATCTGTTATATTTTATCTTCCTTGGACTTGTGATTGACTTCATATTCTACGGATGGTTTCGAAGTTGTGAATTTTAAAAAAAATAACAACCTTATTA
TARGET=85,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.3489
PRIMER_LEFT_PENALTY=3.311546
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=GTTTCGTCATTAACAACTTCACTCC
PRIMER_RIGHT_SEQUENCE=TTCACCACCATACACAGCTTTC
PRIMER_LEFT=40,25
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=60.312
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=3.4406
PRIMER_LEFT_1_PENALTY=3.311546
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=GTTTCGTCATTAACAACTTCACTCC
PRIMER_RIGHT_1_SEQUENCE=ACAGCTTTCACCGGAGATTTTA
PRIMER_LEFT_1=40,25
PRIMER_RIGHT_1=306,22
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=3.5608
PRIMER_LEFT_2_PENALTY=3.311546
PRIMER_RIGHT_2_PENALTY=0.249232
PRIMER_LEFT_2_SEQUENCE=GTTTCGTCATTAACAACTTCACTCC
PRIMER_RIGHT_2_SEQUENCE=CAGCTTTCACCGGAGATTTTAG
PRIMER_LEFT_2=40,25
PRIMER_RIGHT_2=305,22
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=60.249
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=3.5952
PRIMER_LEFT_3_PENALTY=3.311546
PRIMER_RIGHT_3_PENALTY=0.283676
PRIMER_LEFT_3_SEQUENCE=GTTTCGTCATTAACAACTTCACTCC
PRIMER_RIGHT_3_SEQUENCE=CGAAATTCACCACCATACACAG
PRIMER_LEFT_3=40,25
PRIMER_RIGHT_3=324,22
PRIMER_LEFT_3_TM=60.312
PRIMER_RIGHT_3_TM=60.284
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=3.6204
PRIMER_LEFT_4_PENALTY=3.311546
PRIMER_RIGHT_4_PENALTY=0.308871
PRIMER_LEFT_4_SEQUENCE=GTTTCGTCATTAACAACTTCACTCC
PRIMER_RIGHT_4_SEQUENCE=GGGAGAAGAAGAAGAAGGGAGA
PRIMER_LEFT_4=40,25
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=60.309
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_03638 Ps_contig_mira-and-tgicl-ass_franssen_30602
SEQUENCE=TAGAAATTNTTGGTTTGTTAATATTTTTGTCTGTCTACAAGTTTCGTCATTAACAACTTCACTCCTTTTCAATATTAGAGATAACTTCTTCTTCTTCTTCTCTCCCTCTTTCATTCATTCAACAAATAACAATGTCTCATTCTTCTTTTCTCACTCCCTCTTCTTCTCTTACCTTCAATTCCCCTCTTCTTTCCCCTCTCCCTTCTTCTTCTTCTCCCCTCTCTCACAGTTCTTCTCTTCTCTCCGGGAGATTGAGTAAATCTGTAGGATTTAGAAAGACTACTCTAAAATCTCCGGTGAAAGCTGTGTATGGTGGTGAATTTCGGGGACCTGAGAGGAGTTTTCGTCAGGGTATTTGGTCCATAAGGGAGGATTTGGGAGTGCCGTCTTCGCCTTATTTGCCTGTCTATGCTCAAGGACAAGGGCCGCCTCCAATGGTTCAGGAGCGTTTCCAGAGTGTTATAAGCCAGCTTTTCCAATATAGAATAATCCGTTGTGGTGGAGCAGTTGATGATGATATGGCAAATATCATAGTTTCTCAGCTCCTTTATCTTGATGCTGTTGATCCAAATAAGGATATTGTCATGTATATAAACTCTCCGGGAGGATCGGTTACAGCTGGTATGGCAATATTTGATACAATGAGGCATATCAGACCTAATGTGTCCACTGTCTGTGTTGGATTGGCGGCTAGTATGGGAGCTTTTCTGCTTAGCGCAGGGACCAAAGGAAAAAGATTCAGCTTGCCAAATTCAAGGATAATGATTCATCAACCGCTTGGTGGTTTTCAAGGAGGGCAATCTGACATAGATATTCAGGCAAATGAAATGCTTCATCACAAGGCAAACCTGAATGGATATCTGTCCTATCATACTGGTCAAAGTTATGACAAAATTAACCAGGATACTGACCGCGATTTTTTCATGAGCGCAAAAGAAGCTAAAGAATATGGACTCATTGATGGTATCATTATGAATCCTCTCAAAGCTTTTCAGCCTTTGGCTGCTGCAGAAGAGGGTGCAGATCGAGCTATTGCCTGACCTCGATCCAAGGTATAAAAAAACAAGAATGTTGTTTTTGGTTGGCTGCGCTTAGTATAGCTGAATTTTTCACAGATGTCGATATCTGTATAATGGTGTACTGAGAAATGAAGCCAAAGTGATACTTAGTGTAATGTATTGCCTCAAAGTTCATACTTTTAGAATCTTCCTTAAGGGATCCTGGATTCACATTTTTCCTTCACAGGCATTCTATTTTCTTGTGATAACGGATGTCTGGTGTGATCTGTTATATTTTATCTTCCTTGGACTTGTGATTGACTTCATATTCTACGGATGGTTTCGAAGTTGTGAATTTTAAAAAAAATAACAACCTTATTA
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.3403
PRIMER_LEFT_PENALTY=3.303004
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=TTCTTCTTCTTCTTCTCTCCCTCTT
PRIMER_RIGHT_SEQUENCE=TTCACCACCATACACAGCTTTC
PRIMER_LEFT=85,25
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=59.697
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=0.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=3.3489
PRIMER_LEFT_1_PENALTY=3.311546
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=GTTTCGTCATTAACAACTTCACTCC
PRIMER_RIGHT_1_SEQUENCE=TTCACCACCATACACAGCTTTC
PRIMER_LEFT_1=40,25
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=3.3713
PRIMER_LEFT_2_PENALTY=3.303004
PRIMER_RIGHT_2_PENALTY=0.068302
PRIMER_LEFT_2_SEQUENCE=TTCTTCTTCTTCTTCTCTCCCTCTT
PRIMER_RIGHT_2_SEQUENCE=CCTTATGGACCAAATACCCTGA
PRIMER_LEFT_2=85,25
PRIMER_RIGHT_2=367,22
PRIMER_LEFT_2_TM=59.697
PRIMER_RIGHT_2_TM=60.068
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=0.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=3.3855
PRIMER_LEFT_3_PENALTY=3.303004
PRIMER_RIGHT_3_PENALTY=0.082508
PRIMER_LEFT_3_SEQUENCE=TTCTTCTTCTTCTTCTCTCCCTCTT
PRIMER_RIGHT_3_SEQUENCE=AATCCTCCCTTATGGACCAAAT
PRIMER_LEFT_3=85,25
PRIMER_RIGHT_3=374,22
PRIMER_LEFT_3_TM=59.697
PRIMER_RIGHT_3_TM=59.917
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=0.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=3.3869
PRIMER_LEFT_4_PENALTY=3.303004
PRIMER_RIGHT_4_PENALTY=0.083883
PRIMER_LEFT_4_SEQUENCE=TTCTTCTTCTTCTTCTCTCCCTCTT
PRIMER_RIGHT_4_SEQUENCE=CTGACGAAAACTCCTCTCAGGT
PRIMER_LEFT_4=85,25
PRIMER_RIGHT_4=349,22
PRIMER_LEFT_4_TM=59.697
PRIMER_RIGHT_4_TM=59.916
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=0.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_03639 Ps_contig_mira-and-tgicl-ass_franssen_30602
SEQUENCE=TAGAAATTNTTGGTTTGTTAATATTTTTGTCTGTCTACAAGTTTCGTCATTAACAACTTCACTCCTTTTCAATATTAGAGATAACTTCTTCTTCTTCTTCTCTCCCTCTTTCATTCATTCAACAAATAACAATGTCTCATTCTTCTTTTCTCACTCCCTCTTCTTCTCTTACCTTCAATTCCCCTCTTCTTTCCCCTCTCCCTTCTTCTTCTTCTCCCCTCTCTCACAGTTCTTCTCTTCTCTCCGGGAGATTGAGTAAATCTGTAGGATTTAGAAAGACTACTCTAAAATCTCCGGTGAAAGCTGTGTATGGTGGTGAATTTCGGGGACCTGAGAGGAGTTTTCGTCAGGGTATTTGGTCCATAAGGGAGGATTTGGGAGTGCCGTCTTCGCCTTATTTGCCTGTCTATGCTCAAGGACAAGGGCCGCCTCCAATGGTTCAGGAGCGTTTCCAGAGTGTTATAAGCCAGCTTTTCCAATATAGAATAATCCGTTGTGGTGGAGCAGTTGATGATGATATGGCAAATATCATAGTTTCTCAGCTCCTTTATCTTGATGCTGTTGATCCAAATAAGGATATTGTCATGTATATAAACTCTCCGGGAGGATCGGTTACAGCTGGTATGGCAATATTTGATACAATGAGGCATATCAGACCTAATGTGTCCACTGTCTGTGTTGGATTGGCGGCTAGTATGGGAGCTTTTCTGCTTAGCGCAGGGACCAAAGGAAAAAGATTCAGCTTGCCAAATTCAAGGATAATGATTCATCAACCGCTTGGTGGTTTTCAAGGAGGGCAATCTGACATAGATATTCAGGCAAATGAAATGCTTCATCACAAGGCAAACCTGAATGGATATCTGTCCTATCATACTGGTCAAAGTTATGACAAAATTAACCAGGATACTGACCGCGATTTTTTCATGAGCGCAAAAGAAGCTAAAGAATATGGACTCATTGATGGTATCATTATGAATCCTCTCAAAGCTTTTCAGCCTTTGGCTGCTGCAGAAGAGGGTGCAGATCGAGCTATTGCCTGACCTCGATCCAAGGTATAAAAAAACAAGAATGTTGTTTTTGGTTGGCTGCGCTTAGTATAGCTGAATTTTTCACAGATGTCGATATCTGTATAATGGTGTACTGAGAAATGAAGCCAAAGTGATACTTAGTGTAATGTATTGCCTCAAAGTTCATACTTTTAGAATCTTCCTTAAGGGATCCTGGATTCACATTTTTCCTTCACAGGCATTCTATTTTCTTGTGATAACGGATGTCTGGTGTGATCTGTTATATTTTATCTTCCTTGGACTTGTGATTGACTTCATATTCTACGGATGGTTTCGAAGTTGTGAATTTTAAAAAAAATAACAACCTTATTA
TARGET=202,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4454
PRIMER_LEFT_PENALTY=0.408061
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=CTTCAATTCCCCTCTTCTTTCC
PRIMER_RIGHT_SEQUENCE=TTCACCACCATACACAGCTTTC
PRIMER_LEFT=172,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=60.408
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=0.4454
PRIMER_LEFT_1_PENALTY=0.408061
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=CCTTCAATTCCCCTCTTCTTTC
PRIMER_RIGHT_1_SEQUENCE=TTCACCACCATACACAGCTTTC
PRIMER_LEFT_1=171,22
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=60.408
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.4764
PRIMER_LEFT_2_PENALTY=0.408061
PRIMER_RIGHT_2_PENALTY=0.068302
PRIMER_LEFT_2_SEQUENCE=CTTCAATTCCCCTCTTCTTTCC
PRIMER_RIGHT_2_SEQUENCE=CCTTATGGACCAAATACCCTGA
PRIMER_LEFT_2=172,22
PRIMER_RIGHT_2=367,22
PRIMER_LEFT_2_TM=60.408
PRIMER_RIGHT_2_TM=60.068
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.4764
PRIMER_LEFT_3_PENALTY=0.408061
PRIMER_RIGHT_3_PENALTY=0.068302
PRIMER_LEFT_3_SEQUENCE=CCTTCAATTCCCCTCTTCTTTC
PRIMER_RIGHT_3_SEQUENCE=CCTTATGGACCAAATACCCTGA
PRIMER_LEFT_3=171,22
PRIMER_RIGHT_3=367,22
PRIMER_LEFT_3_TM=60.408
PRIMER_RIGHT_3_TM=60.068
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=0.4906
PRIMER_LEFT_4_PENALTY=0.408061
PRIMER_RIGHT_4_PENALTY=0.082508
PRIMER_LEFT_4_SEQUENCE=CTTCAATTCCCCTCTTCTTTCC
PRIMER_RIGHT_4_SEQUENCE=AATCCTCCCTTATGGACCAAAT
PRIMER_LEFT_4=172,22
PRIMER_RIGHT_4=374,22
PRIMER_LEFT_4_TM=60.408
PRIMER_RIGHT_4_TM=59.917
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_03640 Ps_contig_mira-and-tgicl-ass_franssen_30606
SEQUENCE=CATARACACAATTAACCTAACATTTTTAACTTTATTAATTAACCATTACATGTAAATATTCAAATACACATGATATTTAGCACAATTTAAACTCAAATATATATTTATAAAAACAGGATTAATAACCATAATCCACTACCAATCTATAATCAAACATGGCATTTACAACGCCAGATTTACTAATCTGATCTATCCCTTACACAGAGTTACATACATACATAATGATATTATACATTATGCAACTAAATTATGGTTGTAATCTAAGCAGATGAAAGAGAAGGGTTTTTCGTATTCAATCTGAGTTTTCTTATTCTTAAAATATCACAATCCAAACCGCAACCGCACTGTGGAGAATCAAAGAAACTATCTAACGGAAAAGAAGCAGTAGCGACTCCAACAGAGAAATCAAGGTCATCTCCTTTTCTAACAACTTCAAGAATGTTTACCCAGAAGAATAGCACTTTGACGCTTATACCCCTGAGATCTGTAAGTTTGTTATCAGTTATGTGGCCTGTGATGGTTGAATTGTAGCTCAATTGATACGAACCTTGTAAGGAGAAACGGCACGAACCGTTCAGATCGGCGCGGAAATTGCCACTTGATTCGTCGAGTTCGTAGCGTGTTACGCCTTGTGGGAGAATTCCCTCTGGAAAATTGAAGTTTCGAAGGGCTTCGTAGGCGGTTGGTTCGGCGGAGAGGGTTAGCGCCGCCGTTAGGAGGAGGAGGGGGACGGCGAGGTTGGTCATGCGGTGCGGTGTGGTGTGGTGTGGTGTACCCCTGTTTGTTGGGAATGGAATTTACAATTGTTTGGGTTTGTTTGGGTTNCCGCGTTTTTAATTTGTGAAATCCCAAC
TARGET=208,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03641 Ps_contig_mira-and-tgicl-ass_franssen_30606
SEQUENCE=CATARACACAATTAACCTAACATTTTTAACTTTATTAATTAACCATTACATGTAAATATTCAAATACACATGATATTTAGCACAATTTAAACTCAAATATATATTTATAAAAACAGGATTAATAACCATAATCCACTACCAATCTATAATCAAACATGGCATTTACAACGCCAGATTTACTAATCTGATCTATCCCTTACACAGAGTTACATACATACATAATGATATTATACATTATGCAACTAAATTATGGTTGTAATCTAAGCAGATGAAAGAGAAGGGTTTTTCGTATTCAATCTGAGTTTTCTTATTCTTAAAATATCACAATCCAAACCGCAACCGCACTGTGGAGAATCAAAGAAACTATCTAACGGAAAAGAAGCAGTAGCGACTCCAACAGAGAAATCAAGGTCATCTCCTTTTCTAACAACTTCAAGAATGTTTACCCAGAAGAATAGCACTTTGACGCTTATACCCCTGAGATCTGTAAGTTTGTTATCAGTTATGTGGCCTGTGATGGTTGAATTGTAGCTCAATTGATACGAACCTTGTAAGGAGAAACGGCACGAACCGTTCAGATCGGCGCGGAAATTGCCACTTGATTCGTCGAGTTCGTAGCGTGTTACGCCTTGTGGGAGAATTCCCTCTGGAAAATTGAAGTTTCGAAGGGCTTCGTAGGCGGTTGGTTCGGCGGAGAGGGTTAGCGCCGCCGTTAGGAGGAGGAGGGGGACGGCGAGGTTGGTCATGCGGTGCGGTGTGGTGTGGTGTGGTGTACCCCTGTTTGTTGGGAATGGAATTTACAATTGTTTGGGTTTGTTTGGGTTNCCGCGTTTTTAATTTGTGAAATCCCAAC
TARGET=715,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03642 Ps_contig_mira-and-tgicl-ass_franssen_30606
SEQUENCE=CATARACACAATTAACCTAACATTTTTAACTTTATTAATTAACCATTACATGTAAATATTCAAATACACATGATATTTAGCACAATTTAAACTCAAATATATATTTATAAAAACAGGATTAATAACCATAATCCACTACCAATCTATAATCAAACATGGCATTTACAACGCCAGATTTACTAATCTGATCTATCCCTTACACAGAGTTACATACATACATAATGATATTATACATTATGCAACTAAATTATGGTTGTAATCTAAGCAGATGAAAGAGAAGGGTTTTTCGTATTCAATCTGAGTTTTCTTATTCTTAAAATATCACAATCCAAACCGCAACCGCACTGTGGAGAATCAAAGAAACTATCTAACGGAAAAGAAGCAGTAGCGACTCCAACAGAGAAATCAAGGTCATCTCCTTTTCTAACAACTTCAAGAATGTTTACCCAGAAGAATAGCACTTTGACGCTTATACCCCTGAGATCTGTAAGTTTGTTATCAGTTATGTGGCCTGTGATGGTTGAATTGTAGCTCAATTGATACGAACCTTGTAAGGAGAAACGGCACGAACCGTTCAGATCGGCGCGGAAATTGCCACTTGATTCGTCGAGTTCGTAGCGTGTTACGCCTTGTGGGAGAATTCCCTCTGGAAAATTGAAGTTTCGAAGGGCTTCGTAGGCGGTTGGTTCGGCGGAGAGGGTTAGCGCCGCCGTTAGGAGGAGGAGGGGGACGGCGAGGTTGGTCATGCGGTGCGGTGTGGTGTGGTGTGGTGTACCCCTGTTTGTTGGGAATGGAATTTACAATTGTTTGGGTTTGTTTGGGTTNCCGCGTTTTTAATTTGTGAAATCCCAAC
TARGET=754,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03643 Ps_contig_mira-and-tgicl-ass_franssen_30607
SEQUENCE=GGTATCAAACGACAAGAAGTTGAGGGTTTTCATATTGCAGATGAGGCCAAGTCCAAGGGAAAGGAAGAGGCAGGGGATGAGTTATGATACTCGTAATGATTGCACGCTTTTGTCAGCATTGTTTTACAATTTCAGCATGTTATCTGAACCGTGGGCCTTCTAATATACATGATTAGTATACACTAAATTTTGTAAGTTTTAGCTGTATCGAACATTTAATGAATTGATGAGCTGGAATAAAGGATTTAAGGTTTGTTTGTTTTAATTTCAGGGAGTTTGATGAGCT
TARGET=251,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8907
PRIMER_LEFT_PENALTY=0.130883
PRIMER_RIGHT_PENALTY=0.759842
PRIMER_LEFT_SEQUENCE=TTTCAGCATGTTATCTGAACCG
PRIMER_RIGHT_SEQUENCE=AGCTCATCAAACTCCCTGAAAT
PRIMER_LEFT=129,22
PRIMER_RIGHT=285,22
PRIMER_LEFT_TM=60.131
PRIMER_RIGHT_TM=59.240
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.9534
PRIMER_LEFT_1_PENALTY=0.193532
PRIMER_RIGHT_1_PENALTY=0.759842
PRIMER_LEFT_1_SEQUENCE=TACTCGTAATGATTGCACGCTT
PRIMER_RIGHT_1_SEQUENCE=AGCTCATCAAACTCCCTGAAAT
PRIMER_LEFT_1=87,22
PRIMER_RIGHT_1=285,22
PRIMER_LEFT_1_TM=59.806
PRIMER_RIGHT_1_TM=59.240
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=199
PRIMER_PAIR_PENALTY_2=1.0022
PRIMER_LEFT_2_PENALTY=0.242357
PRIMER_RIGHT_2_PENALTY=0.759842
PRIMER_LEFT_2_SEQUENCE=ACCGTGGGCCTTCTAATATACA
PRIMER_RIGHT_2_SEQUENCE=AGCTCATCAAACTCCCTGAAAT
PRIMER_LEFT_2=147,22
PRIMER_RIGHT_2=285,22
PRIMER_LEFT_2_TM=59.758
PRIMER_RIGHT_2_TM=59.240
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=1.0667
PRIMER_LEFT_3_PENALTY=0.306868
PRIMER_RIGHT_3_PENALTY=0.759842
PRIMER_LEFT_3_SEQUENCE=GGCAGGGGATGAGTTATGATAC
PRIMER_RIGHT_3_SEQUENCE=AGCTCATCAAACTCCCTGAAAT
PRIMER_LEFT_3=68,22
PRIMER_RIGHT_3=285,22
PRIMER_LEFT_3_TM=59.693
PRIMER_RIGHT_3_TM=59.240
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.3000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=1.1021
PRIMER_LEFT_4_PENALTY=0.342304
PRIMER_RIGHT_4_PENALTY=0.759842
PRIMER_LEFT_4_SEQUENCE=GTAATGATTGCACGCTTTTGTC
PRIMER_RIGHT_4_SEQUENCE=AGCTCATCAAACTCCCTGAAAT
PRIMER_LEFT_4=92,22
PRIMER_RIGHT_4=285,22
PRIMER_LEFT_4_TM=59.658
PRIMER_RIGHT_4_TM=59.240
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_03644 Ps_contig_mira-and-tgicl-ass_franssen_30609
SEQUENCE=CTAACCCTGCAGTTACCACTCAAAAACCAAGAAAGATGAATAGAAGGATTGAAAATAACTATAATCCACTGTCATTTATAAGTTTCTTATAAATAAAAAAGGGCAAAAGGTTCTGAAAACAATACCCAACCAATTTAGAACAAACAAACAGAAAAACTACCAAAAATTCAAAATCTAACACCCAAACAAAACCCTAAAAATCTCTTAAATTATATCAGTCAGCCTTAGAAGCAGTAGCGGCAGCCTGCCCCCTAAGTCGACCAGTTCTCTTAGCAGCAATAAGACCAACCTTCTGCCCGGGAGGAGCATCACGTCTAACAGTACTAGCATGTCCAATATGCTGGTGGTTACCTCCTCCATGAGGATGTTCAACTGGATTCATAGCCACACCACGAACCTTAGGCCAACAATTCCTCTTTACTCTAAATTTGTGGTAAGCATTACCGGCCTTAAGAAGTGGCTTCTCTGTTCTTCCTCCACCAGCAACTTGGCCAATCATAGCCCTGCAACCACTTGGAATAATCTTTTTTGCCCCAGATGGAAGCTTAACCCTAGATGTATCATTATCAGGATTATGACTAATAACAATAGCATAATCACCAGAACATCTAGCGAAAGCACCGCGATCACCAACATGGTGTTCAACGTTACAAATAACAGCTCCTTCAGGAATAGACCTAACAGGCAAAACATTACCAACAACAAGAGTAGCTTTCTTCCCGCAATAAATAAACTGTCCAGTGTAGAGTCCTTCAGCAGAAACAAAAAGCTCGTTCTGTTTCTTATACCGGAATGGGTGACGGAAAGTGACCTTGGCAAGAGGTGCACCGCGACCGGGGTCGTGGATGATGTCGGTGATTACTCCCTTGAGATAACCGTTTCTTTCACCGAAATCGAGACTGCGGAATCTTGCGGGTCCTTTGCGGTGGTGGGTGTGGGATTTGAAGATTGATCCCGCACCCTTACGTTGTGCTCTGATAACCCTACCCATTCCGACTAACCTCCGAGGACTCTATCTACCGTGCGGCGAAAATGGAGGAGGAAATGGGTCCCCGAGTAACTAGGGTTTTGGTTATAATATATAGTACGCGCTCTTTTTTGTNCCCCGAC
TARGET=139,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5398
PRIMER_LEFT_PENALTY=0.328883
PRIMER_RIGHT_PENALTY=0.210886
PRIMER_LEFT_SEQUENCE=TAACCCTGCAGTTACCACTCAA
PRIMER_RIGHT_SEQUENCE=GCAGAAGGTTGGTCTTATTGCT
PRIMER_LEFT=1,22
PRIMER_RIGHT=295,22
PRIMER_LEFT_TM=59.671
PRIMER_RIGHT_TM=59.789
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=0.5398
PRIMER_LEFT_1_PENALTY=0.328883
PRIMER_RIGHT_1_PENALTY=0.210886
PRIMER_LEFT_1_SEQUENCE=TAACCCTGCAGTTACCACTCAA
PRIMER_RIGHT_1_SEQUENCE=GAAGGTTGGTCTTATTGCTGCT
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=292,22
PRIMER_LEFT_1_TM=59.671
PRIMER_RIGHT_1_TM=59.789
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.5398
PRIMER_LEFT_2_PENALTY=0.328883
PRIMER_RIGHT_2_PENALTY=0.210886
PRIMER_LEFT_2_SEQUENCE=TAACCCTGCAGTTACCACTCAA
PRIMER_RIGHT_2_SEQUENCE=AGAAGGTTGGTCTTATTGCTGC
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=59.671
PRIMER_RIGHT_2_TM=59.789
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.7770
PRIMER_LEFT_3_PENALTY=0.566078
PRIMER_RIGHT_3_PENALTY=0.210886
PRIMER_LEFT_3_SEQUENCE=TGCAGTTACCACTCAAAAACCA
PRIMER_RIGHT_3_SEQUENCE=GCAGAAGGTTGGTCTTATTGCT
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=295,22
PRIMER_LEFT_3_TM=60.566
PRIMER_RIGHT_3_TM=59.789
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=0.7770
PRIMER_LEFT_4_PENALTY=0.566078
PRIMER_RIGHT_4_PENALTY=0.210886
PRIMER_LEFT_4_SEQUENCE=TGCAGTTACCACTCAAAAACCA
PRIMER_RIGHT_4_SEQUENCE=AGAAGGTTGGTCTTATTGCTGC
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=293,22
PRIMER_LEFT_4_TM=60.566
PRIMER_RIGHT_4_TM=59.789
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_03645 Ps_contig_mira-and-tgicl-ass_franssen_30610
SEQUENCE=CTACACCTTACACTTAAAAACRGACAATTTCACTCGACAAAGGGAAAAATCAAAACAAACCCAAACCAAAAAACCCTAAAGTGAAAATGGCGAGTTCAGTGGAGAAGGAGGGTTCTGCTTCTAAAGCTGCATCTGCAGTTGAGGAGCCTGTTTCTTTGGAGCTTCCAGCTCCATCTGGTTGGACGAAAAAGTTCTTTCCAAAAAAGTCTGGAACTCCAAAGAAAAATGAGATTGTGTTCACTGCACCAACAGGAGAGGAGATCCATAACAAGAGGCAGCTGGATAAGTATTTGAAAGCAAACCCCGGTGGCCCAAACATCTCAGAATTCGATTGGGGCACTGGGGAGACTCCAAGAAGATCATCAAGAATCAGTGAGAAGGTCAAGGCATCTCCTCCATTAGAACCAAAAAGTGAGCCCTCTAAGAAGCGTTACAAAAAGTCAGCTTCAAAGAAAGAAGCTTCTGAGGATGAAGAGGAGACAAAAGATGTGGAAATGCAAGAAGCCGATGAAACTAAGGATGATAAAGATTTAGAGCAGGAAAAGAAAGTTGTTAACGAAGAACAAGGTGATAAGGGACCGGAGGGTGCAGATGTCAAAGAGTCCATTCAACCTGGTGAGAATACTGATATAGCAAAAGATGAGGGGAAGTCCGACACTGCCGATGGAGAGTTACAAGCATCCAAAGAGAAAATTGATGACAAAGGAGCTGAGGGGTCTGAAGCTGTTCAGAATAAGGATGAAGAAATGATTGAACAACCACAGCCACAGGAGGAGCCAAACCAAGCTGTTCAGAGTAAGGAGGAAGAAAAGATTGAACAACCACAGGAGGAGACGAACCAAGTTGTTCAGAATGAGGAAGAAAAGATTGAACAACCACAGGAGGAGACAAACCATGCTGTTCAGATCGAGGAAGAAAAGATTGAACAACCACAGCCACAAGAGGAGGCAAAGCAAGCTGTTCAGAATAATGATGAAGAAAAGATTGAACAACCACAGGAGGAGACGAAGCAAGCTGTTCAGAGCAAGGATGAAGAAAAGATCGAACAACCACACGAGGAGACAAAACAAGATGGTGGATCTGGGGAAGCAGAGAAACCAGAAGCTGCCCTTAATGCTGAGAAACCAGCTGAATTTGAAGGGGAAAATAAAGAGGAAGATAACAGAATCGCTCAAGAGGCTGAAGGAAATATCAAGGAGAAAGAAGCTACAAAAGTGCACGGTGAAGAAAATTACAAGATTCATGATATGAACAAGACTGAGTCAGAGGTGACCGTGAATGGAAGCTGAATTGTTGAGACCAGAGAACTTGAACAAGGAATAGAATAAACTCTTGGCTTCTATCTTCTGTCACTGTGATGCCTTTAGATCTGTCCGAGGATGACTGTAGTTTTTGTTGTTGTTATTTATCTGATTGTTCATTGAAATGACTATAGACAATCATAGAGTTAGCCCTATAATGTACTACTATCTATCTATGTTCTGGAGAGACATGTAGCTTCTAGTTCCCATGTAACATTTGTATTGTATGATACCTGTAATCTTATGTGAGACCTTGATTTTAAATATGTCTTGTAGTGTCATCAAATGTAATGCGCCAGGTCTCATTTATCTTGAGGCTGTTTACATGTTTTTATTTCATTGCATTGTTTCAGT
TARGET=1467,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03646 Ps_contig_mira-and-tgicl-ass_franssen_30614
SEQUENCE=AGTTAAGTAAAAAACAGCAATCTACAAAATTGACTTCAAAATTCAAACCAAAACAAGTTACTAACAGTTACTACACAGTAAATCATTAGTACACACATTCGTGAAGTTGTACTTCCCTATTTATACAAAACCCATAAACATTTCAAATCTTCATTTAAAAAAAAACATTCATAATCATACACTTTCATTCCATTGCCAAATCAAGTACATATCTTCTCTTGCTTAGTAGTCCCAAGTGTAAATGCAGAAAGCTCTGTACTCTTCAATACTCGAAGCCTCTTCACTGTTGATACAAACATATGCCATGGGACATCACCAACAAGCATCCTGTCTCCTTCATTATCCTCATATACAAGAGTATATTCTCCACTTCCATCCAATATTCCACTTATCACTTTTTCTTCATCTTCATTTTTCTTACTTCCACAAGAATCTCCTTGAGCTTTAAGGAGTCCTCTAAAGAGTTCATCCACAGCTATAGAGAGATTTTCATAGCTATGATAAGCTTTGAGATCAACTTTTCTACCAATAGGAACACCATCCATGTTGATTTTAACAAACAGCCCTTTACCATAGTTGTTGTCAATAGGTTTTTTACCAGCAACAACCTTGTTGTGATGTTGTGTCTGAGATTCAGTTTGTGATGTTGGTTTAGAATTGCTGCTTGTTGTTGCAAGATTTTTCCTGAATGAACGAATTGGTGGCCAACCTACTACTGGCCCAGGAGCAGTTCTTTTCTGAGAAGTGTTGGAAACAGCTGTAACTTTGTTATTATCACCATTCTGTAACTCTACTAGCTTAGGACAAGAGGCTTGTGATGATGAAGATTCTTTGCCTCTCACATGTTGGTTTGATGGAAAGTGAAGAAATGATGTATTGTTTGTTGAAAAGTAATTACTGCCAAGAGAGAGTTTAGAGTCATGATTATCAAGATCTCTTTGTTTGTTGTTGTTGGTGGTGTGAAACCAGTTTTCAACACCAGGTGGAGCAAGCCTTAGTTCTAGCTTTCTTTCTTCTGAAGATTCTCTCTCATTTCTTGACATTTCATTCATCACTAGAGAAAGAAAATAAACCCTTATGCGAAAAAAGAACAAGAGAGTGTTAGAGAGAGAAAGATTCTACAATGAAAGAGTGAGAAAGTAGAAATATTATAGAGCAAAGAGAGAGAGTGATAATAATAATAGTAGCAAGAGATTGTGAAATCCCCNYGTACTGGTTTGCTTTAAGTCTTTTATTTTGCACGCTTAGAAGGTCCTATAGTTTCTCTCTCCTCTTAGATAAAGGGAAAGGAG
TARGET=943,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03647 Ps_contig_mira-and-tgicl-ass_franssen_30614
SEQUENCE=AGTTAAGTAAAAAACAGCAATCTACAAAATTGACTTCAAAATTCAAACCAAAACAAGTTACTAACAGTTACTACACAGTAAATCATTAGTACACACATTCGTGAAGTTGTACTTCCCTATTTATACAAAACCCATAAACATTTCAAATCTTCATTTAAAAAAAAACATTCATAATCATACACTTTCATTCCATTGCCAAATCAAGTACATATCTTCTCTTGCTTAGTAGTCCCAAGTGTAAATGCAGAAAGCTCTGTACTCTTCAATACTCGAAGCCTCTTCACTGTTGATACAAACATATGCCATGGGACATCACCAACAAGCATCCTGTCTCCTTCATTATCCTCATATACAAGAGTATATTCTCCACTTCCATCCAATATTCCACTTATCACTTTTTCTTCATCTTCATTTTTCTTACTTCCACAAGAATCTCCTTGAGCTTTAAGGAGTCCTCTAAAGAGTTCATCCACAGCTATAGAGAGATTTTCATAGCTATGATAAGCTTTGAGATCAACTTTTCTACCAATAGGAACACCATCCATGTTGATTTTAACAAACAGCCCTTTACCATAGTTGTTGTCAATAGGTTTTTTACCAGCAACAACCTTGTTGTGATGTTGTGTCTGAGATTCAGTTTGTGATGTTGGTTTAGAATTGCTGCTTGTTGTTGCAAGATTTTTCCTGAATGAACGAATTGGTGGCCAACCTACTACTGGCCCAGGAGCAGTTCTTTTCTGAGAAGTGTTGGAAACAGCTGTAACTTTGTTATTATCACCATTCTGTAACTCTACTAGCTTAGGACAAGAGGCTTGTGATGATGAAGATTCTTTGCCTCTCACATGTTGGTTTGATGGAAAGTGAAGAAATGATGTATTGTTTGTTGAAAAGTAATTACTGCCAAGAGAGAGTTTAGAGTCATGATTATCAAGATCTCTTTGTTTGTTGTTGTTGGTGGTGTGAAACCAGTTTTCAACACCAGGTGGAGCAAGCCTTAGTTCTAGCTTTCTTTCTTCTGAAGATTCTCTCTCATTTCTTGACATTTCATTCATCACTAGAGAAAGAAAATAAACCCTTATGCGAAAAAAGAACAAGAGAGTGTTAGAGAGAGAAAGATTCTACAATGAAAGAGTGAGAAAGTAGAAATATTATAGAGCAAAGAGAGAGAGTGATAATAATAATAGTAGCAAGAGATTGTGAAATCCCCNYGTACTGGTTTGCTTTAAGTCTTTTATTTTGCACGCTTAGAAGGTCCTATAGTTTCTCTCTCCTCTTAGATAAAGGGAAAGGAG
TARGET=1160,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03648 Ps_contig_mira-and-tgicl-ass_franssen_30614
SEQUENCE=AGTTAAGTAAAAAACAGCAATCTACAAAATTGACTTCAAAATTCAAACCAAAACAAGTTACTAACAGTTACTACACAGTAAATCATTAGTACACACATTCGTGAAGTTGTACTTCCCTATTTATACAAAACCCATAAACATTTCAAATCTTCATTTAAAAAAAAACATTCATAATCATACACTTTCATTCCATTGCCAAATCAAGTACATATCTTCTCTTGCTTAGTAGTCCCAAGTGTAAATGCAGAAAGCTCTGTACTCTTCAATACTCGAAGCCTCTTCACTGTTGATACAAACATATGCCATGGGACATCACCAACAAGCATCCTGTCTCCTTCATTATCCTCATATACAAGAGTATATTCTCCACTTCCATCCAATATTCCACTTATCACTTTTTCTTCATCTTCATTTTTCTTACTTCCACAAGAATCTCCTTGAGCTTTAAGGAGTCCTCTAAAGAGTTCATCCACAGCTATAGAGAGATTTTCATAGCTATGATAAGCTTTGAGATCAACTTTTCTACCAATAGGAACACCATCCATGTTGATTTTAACAAACAGCCCTTTACCATAGTTGTTGTCAATAGGTTTTTTACCAGCAACAACCTTGTTGTGATGTTGTGTCTGAGATTCAGTTTGTGATGTTGGTTTAGAATTGCTGCTTGTTGTTGCAAGATTTTTCCTGAATGAACGAATTGGTGGCCAACCTACTACTGGCCCAGGAGCAGTTCTTTTCTGAGAAGTGTTGGAAACAGCTGTAACTTTGTTATTATCACCATTCTGTAACTCTACTAGCTTAGGACAAGAGGCTTGTGATGATGAAGATTCTTTGCCTCTCACATGTTGGTTTGATGGAAAGTGAAGAAATGATGTATTGTTTGTTGAAAAGTAATTACTGCCAAGAGAGAGTTTAGAGTCATGATTATCAAGATCTCTTTGTTTGTTGTTGTTGGTGGTGTGAAACCAGTTTTCAACACCAGGTGGAGCAAGCCTTAGTTCTAGCTTTCTTTCTTCTGAAGATTCTCTCTCATTTCTTGACATTTCATTCATCACTAGAGAAAGAAAATAAACCCTTATGCGAAAAAAGAACAAGAGAGTGTTAGAGAGAGAAAGATTCTACAATGAAAGAGTGAGAAAGTAGAAATATTATAGAGCAAAGAGAGAGAGTGATAATAATAATAGTAGCAAGAGATTGTGAAATCCCCNYGTACTGGTTTGCTTTAAGTCTTTTATTTTGCACGCTTAGAAGGTCCTATAGTTTCTCTCTCCTCTTAGATAAAGGGAAAGGAG
TARGET=1172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03649 Ps_contig_mira-and-tgicl-ass_franssen_30618
SEQUENCE=AGTACGCGGGACTCAANCTGCTATAAGGGAAGATCTTAATCTATCTCTTGCTCAGTTTTCAATGTTTGGTTCTTTGGTGACCATTGGTGCAATGCTTGGAGCTATAACAAGTGGTAGAATTACTGATTTCATTGGCAGAAAAGGGGTATGTACTATGTATGTCACACTTTATTCTGTCGATTTTTACACACGAATAGAAAAAATTGTGAAACAACAAACAAACAGGCTATGAGAATTTCAACCGGATTTTGCATTATAGGGTGGTTAGCTGTTTTCTTCTCTAAGGTATATATCTTTTGAACAATGTGTTCATCTTTTGAATAGTTGATGACAAAAAAAAAACTTATTGCGTTATTCCGTCTAATTCAGGGCTCTTCCACACTTGATTTAGGAAGATTGTTTACAGGATATGGAATTGGAGTTATCTCATACGTGGTTCCGGTATATATAGCAGAAATAGCACCGAAAAATCTTCGGGGAGGACTCGCAACAACAAATCAACTTATGATTGTTATTGGTTCATCGGTATCATTTTTATTAGGAAGTGTTCTAAATTGGAGAAAACTAGCACTAGCAGGAGTAGCGCCTTGCATTTGCTTGTTGGTTGGTTTGTTCTTCGTTCCCGAGTCTCCTAGATGGCTGGCAAAGGTTGGTCGTGAAAAAGAGTTTCAACTAGCTTTAAGGAAACTTCGTGGTAAAGATGTTGATATTTCTCATGAAGCTATTGAAATTATGGATAATATTGAAACTCTTAAAAACCTTCCTAAGACTAAGATGTTTGATTTGTTCCAAAGCAGATACGTGCGGTCTGTAGTTATCGGCGTTGGACTAATGGCATTTCAACAATCTGTTGGAATTAATGGCATAGGATTCTATACGGCTGAAACTTTTGTTGCAGCAGGGCTTTCTTCAGGAAAAGCTGGTACCATAGCTTATGCTTGTATACAAGTTCCATTTACCATGTTGGGGGCCATTTTGATGGATAAATCCGGAAGAAAGCCTCTTATAACAGTTTCTGCAAGTGGAACATTCTTAGGCTGCTTAATTACCGGAATTGCTTTCGTTTTCAAGGACCAAAGCTTATTGCTTAAGTGGGTGCCAACATTAGCAGTTGCAGGCGTGTTGATCTACATAGCATCATTTTCAATCGGGTTAGGATCAGTTCCTTGGGTGATGATGTCTGAGGTGTTTCCTATAAATGTTAAGGGAATTGCTGGAAGTTTGGTGGTTTTGGTAGCCTGGCTAGGAGCTTGGGTAGTTTCATATACCTTCAATTTTCTTATGAGTTGGAGTTCTCCTGGTACAATGTTTTTGTATGCTGGATGTTCCCTTTTAACCATTCTATTTGTTGCAAAAGTAGTTCCAGAAACCAAAGGAAAAACATTGGAAGAGATTCAAGCTTGCATTAGTTCTTAGAGAATAGGCTAAAAATATATATGAATTGGATACATTTTGGACATTGTATCTCTCATGATTTGCATCAAGTTTAAGGCATGAAGAGACTAGGAAGAATGACATTCATAGCATTATTAGTTTAATTTTAGTTTGGACTTATTTTTTGGTAACAAAATTAGGATATTGTTGTTGCCATTAATTAATTAAACG
TARGET=213,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1178
PRIMER_LEFT_PENALTY=0.107763
PRIMER_RIGHT_PENALTY=0.010077
PRIMER_LEFT_SEQUENCE=TGGCAGAAAAGGGGTATGTACT
PRIMER_RIGHT_SEQUENCE=CCTGAATTAGACGGAATAACGC
PRIMER_LEFT=132,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.1000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.2381
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=TGGCAGAAAAGGGGTATGTACT
PRIMER_RIGHT_1_SEQUENCE=AGTGTGGAAGAGCCCTGAATTA
PRIMER_LEFT_1=132,22
PRIMER_RIGHT_1=382,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.1000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=0.2496
PRIMER_LEFT_2_PENALTY=0.107763
PRIMER_RIGHT_2_PENALTY=0.141882
PRIMER_LEFT_2_SEQUENCE=TGGCAGAAAAGGGGTATGTACT
PRIMER_RIGHT_2_SEQUENCE=CCCTGAATTAGACGGAATAACG
PRIMER_LEFT_2=132,22
PRIMER_RIGHT_2=370,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=59.858
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.1000
PRIMER_RIGHT_2_END_STABILITY=7.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.2731
PRIMER_LEFT_3_PENALTY=0.263023
PRIMER_RIGHT_3_PENALTY=0.010077
PRIMER_LEFT_3_SEQUENCE=ATTGGCAGAAAAGGGGTATGTA
PRIMER_RIGHT_3_SEQUENCE=CCTGAATTAGACGGAATAACGC
PRIMER_LEFT_3=130,22
PRIMER_RIGHT_3=369,22
PRIMER_LEFT_3_TM=59.737
PRIMER_RIGHT_3_TM=59.990
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=0.3934
PRIMER_LEFT_4_PENALTY=0.263023
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=ATTGGCAGAAAAGGGGTATGTA
PRIMER_RIGHT_4_SEQUENCE=AGTGTGGAAGAGCCCTGAATTA
PRIMER_LEFT_4=130,22
PRIMER_RIGHT_4=382,22
PRIMER_LEFT_4_TM=59.737
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_03650 Ps_contig_mira-and-tgicl-ass_franssen_30618
SEQUENCE=AGTACGCGGGACTCAANCTGCTATAAGGGAAGATCTTAATCTATCTCTTGCTCAGTTTTCAATGTTTGGTTCTTTGGTGACCATTGGTGCAATGCTTGGAGCTATAACAAGTGGTAGAATTACTGATTTCATTGGCAGAAAAGGGGTATGTACTATGTATGTCACACTTTATTCTGTCGATTTTTACACACGAATAGAAAAAATTGTGAAACAACAAACAAACAGGCTATGAGAATTTCAACCGGATTTTGCATTATAGGGTGGTTAGCTGTTTTCTTCTCTAAGGTATATATCTTTTGAACAATGTGTTCATCTTTTGAATAGTTGATGACAAAAAAAAAACTTATTGCGTTATTCCGTCTAATTCAGGGCTCTTCCACACTTGATTTAGGAAGATTGTTTACAGGATATGGAATTGGAGTTATCTCATACGTGGTTCCGGTATATATAGCAGAAATAGCACCGAAAAATCTTCGGGGAGGACTCGCAACAACAAATCAACTTATGATTGTTATTGGTTCATCGGTATCATTTTTATTAGGAAGTGTTCTAAATTGGAGAAAACTAGCACTAGCAGGAGTAGCGCCTTGCATTTGCTTGTTGGTTGGTTTGTTCTTCGTTCCCGAGTCTCCTAGATGGCTGGCAAAGGTTGGTCGTGAAAAAGAGTTTCAACTAGCTTTAAGGAAACTTCGTGGTAAAGATGTTGATATTTCTCATGAAGCTATTGAAATTATGGATAATATTGAAACTCTTAAAAACCTTCCTAAGACTAAGATGTTTGATTTGTTCCAAAGCAGATACGTGCGGTCTGTAGTTATCGGCGTTGGACTAATGGCATTTCAACAATCTGTTGGAATTAATGGCATAGGATTCTATACGGCTGAAACTTTTGTTGCAGCAGGGCTTTCTTCAGGAAAAGCTGGTACCATAGCTTATGCTTGTATACAAGTTCCATTTACCATGTTGGGGGCCATTTTGATGGATAAATCCGGAAGAAAGCCTCTTATAACAGTTTCTGCAAGTGGAACATTCTTAGGCTGCTTAATTACCGGAATTGCTTTCGTTTTCAAGGACCAAAGCTTATTGCTTAAGTGGGTGCCAACATTAGCAGTTGCAGGCGTGTTGATCTACATAGCATCATTTTCAATCGGGTTAGGATCAGTTCCTTGGGTGATGATGTCTGAGGTGTTTCCTATAAATGTTAAGGGAATTGCTGGAAGTTTGGTGGTTTTGGTAGCCTGGCTAGGAGCTTGGGTAGTTTCATATACCTTCAATTTTCTTATGAGTTGGAGTTCTCCTGGTACAATGTTTTTGTATGCTGGATGTTCCCTTTTAACCATTCTATTTGTTGCAAAAGTAGTTCCAGAAACCAAAGGAAAAACATTGGAAGAGATTCAAGCTTGCATTAGTTCTTAGAGAATAGGCTAAAAATATATATGAATTGGATACATTTTGGACATTGTATCTCTCATGATTTGCATCAAGTTTAAGGCATGAAGAGACTAGGAAGAATGACATTCATAGCATTATTAGTTTAATTTTAGTTTGGACTTATTTTTTGGTAACAAAATTAGGATATTGTTGTTGCCATTAATTAATTAAACG
TARGET=1590,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03651 Ps_contig_mira-and-tgicl-ass_franssen_30619
SEQUENCE=CAGAGAAACCGGCTTTAGAAGAAAAACAAGAAATGCAATATTYGYCACAAGCAAATGGCAGCAAAACCATCTTTAGAATGGTACAATACACAATGTGATAAGAGGCCTGATAAACATTTGTGGGCTACAAAGAATAAGAATTTCATATTGTGACACACACCGATGTATTACTAAGTCAGAAGACAAATCACACCACACAAATCAAGCAACATACTAAAAAAAGGTCTTATAAAAAATCCTGTTCTATGATGTTCCCGCCGACACATGCAAATGCTTTCGTGACCAACCAAGCCAACACAACTCTTATATATAGACAACAGACAAAGGGACTCGGACTCAAGCGCCCTTGTTACTCTTCCAAGGTTTGATAACACCGACAACTATGATAGCTACTATTATTAACAGGATCACGATGGCAATGCACATCCATTTTCGAGAGTTCTTCTGGAGTTTCTTAGCACTTTGAAGTGCAGTTGTCCCTCTCTGGACATGATCTACTGCATTGTTGACCTGGCTTTCAATGTTGTCTAAAATTTCTCCTTGAGCATCAACTATGACTGCCATGTCGAGGTAAATCTGATGTAAATCAAGAAGTTTTTTCTCGATTTCTTTCACAGCATCGTGTCTCTCCTGAATTTCTTCTACCGTGTTCACTACCTGTCCTCGGCCAGCTTCAAGAATTGCTTGTTGGAAGATTTGCTCGCTGTTTCCAGTTTCTATCAGGTGGTCAATCGTCTCATCATCTGGCCTAGTTCCCGTGACTGTAATAACTCTTCTCTCCACAACCTCACGATATTCATCTTGTATTCTTTGTCTCAGAGTCTGAAACTCTGTCATAAGATCCCTGAATTTTTTAGTCAAGGAATTTGTCATATTCATCCTTGCTCTATCAATACCGGTTCCCTTCTCACAGCCAGGCTTTTGTCTATTGTTCAGATTTTCTCGGTTGATAGCTTCAATTTTCGCTTTGACACCATATGCAATCTTTCCAACTTCATCGATATCTTTTTCCATCCTCTTCTTGATAGCCTTCATGGAAGATGCCTTTGTAACAGCTTTTGATTCCTCATTAGCTTCCTTTAGCTTTTGAAGTAGCACAGATAGCTTCTCAATTTGTTTATCGGCGTCTTGGATCTGTTTATTAAAAGCTTCCATTCCCATATCAGAATTGCTTCCTGGAACCTGCATTCCCATTTCAATATCACCTTGTCTAGAAGGCTGCCCATTAGTAGCCTCACTAACAAATGAATCAGTAAGAAGGTCGTTCATGTTGAATTCAGAGAGAGAGAGGAATGTCGAAAGAAGAGACTAAAACGGCAACAATAATTCTGTAAAACGACAGCGAAGTTATGCTAGGGAGAACGAGGCAACCCACGAACTCTCTCTCAACAACAATGCCTTTTTGTTTTTGTTTTT
TARGET=1279,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03652 Ps_contig_mira-and-tgicl-ass_franssen_30630
SEQUENCE=ATTTCATTGTTTTGTTGTTTCACACTGACCACTCACACCACAAAGTTGTGGTTTATATGCATCCATTCTAATCCACTCCACCCCGATTGGTGTGTCTCACACCAATCTTCTTCTTCTTCTTCTCTCTCTCTTATTTTTCCATGGCGGCAAAACTTTCCACCGCCACAACCTTAAAGCCCTACAATCTCATCCAAACATCAACTTCTTCCCTAAAACACCCCAACAATATTCTTCACCCCCTTAACACCCCCAACAATATTCTTCACCCCCTTAACAAGCTTTCGTCATAAAACACTCCTTCAAACCCATAGAAGATCAAGAACAACACCCTTCACCGTTTGTGTTCTGATGGAGGATCCAAAACACACAACCCAAATAGACACAGAAGAAAAAACAGAACTTCCTAATAATAAACCCAACCCTCAATTTTCAGTATCACAAAAACTGGCAAGAAAGAAGTCACAGAGGTCCACTTACCTTGTTGCCGCTGTAATGTCTAGCTTTGGTGTTACCTCTATGGCAATCTTAGCAGTTTATTATAGATTTTCTTGGCAAATGGAGGGTAGTGGAGAAGTTCCATGGTCTGAAATGTTTGGCACATTTGCCCTCTCAGTGGGTGCTGCTGTGGGTATGGAGTTTTGGGCAAGATGGGCTCATGAGGTTCTATGGCATGCTTCCTTGTGGCACATGCATGAGTCACATCATCGACCAAGAGAAGGTCCATTTGAGCTTAATGATGTTTTTGCAATAATTAACGCTGTCCCTGCAATTGCTCTTCTCTCATACGGTTTTTTCAACAAGGGATTGTTTCCTGGACTCTGTTTTGGTGCAGGTCTTGGAATTACAGTGTTTGGGATTGCTTACATGTTTGTCCATGATGGATTGGTTCATAAGAGATTCCCTGTGGGTCCTATTGCTAATGTGCCTTACTTTACAAGGGTTGCTGCTGCTCATCAACTTCATCATTCAGACAAATTCAAAGGAGTGCCATACGGGCTCTTTTTGGGCCCAAAGGAAGTTGAAGAGGTAGGAGGACTAGAAGAGCTGGAGAAAGAGATAAGTAGAAGAACAAGATCATATACTGGTTCATGACAAATGAATAGTATTATAGTTGTTTTAAAGAATTTTGAGTATGAAGAGCAATTCAATTCAATTAATGAAGTGATTGAATGTGAGTGTTAATTTGTCCAGTTATAGTTATTAATTTCAAGATAATCAAAGCCTGACATTAAACTGNAGTAGGTGTGGGTTCAAGG
TARGET=106,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1349
PRIMER_LEFT_PENALTY=0.111365
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=ACTGACCACTCACACCACAAAG
PRIMER_RIGHT_SEQUENCE=AGGGAAGAAGTTGATGTTTGGA
PRIMER_LEFT=23,22
PRIMER_RIGHT=210,22
PRIMER_LEFT_TM=60.111
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.1349
PRIMER_LEFT_1_PENALTY=0.111365
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=CTGACCACTCACACCACAAAGT
PRIMER_RIGHT_1_SEQUENCE=AGGGAAGAAGTTGATGTTTGGA
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=210,22
PRIMER_LEFT_1_TM=60.111
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.1455
PRIMER_LEFT_2_PENALTY=0.111365
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=CTGACCACTCACACCACAAAGT
PRIMER_RIGHT_2_SEQUENCE=TTGGATGAGATTGTAGGGCTTT
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=193,22
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.1455
PRIMER_LEFT_3_PENALTY=0.111365
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=CTGACCACTCACACCACAAAGT
PRIMER_RIGHT_3_SEQUENCE=TTTGGATGAGATTGTAGGGCTT
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=194,22
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.1455
PRIMER_LEFT_4_PENALTY=0.111365
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=ACTGACCACTCACACCACAAAG
PRIMER_RIGHT_4_SEQUENCE=TTTGGATGAGATTGTAGGGCTT
PRIMER_LEFT_4=23,22
PRIMER_RIGHT_4=194,22
PRIMER_LEFT_4_TM=60.111
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_03653 Ps_contig_mira-and-tgicl-ass_franssen_30630
SEQUENCE=ATTTCATTGTTTTGTTGTTTCACACTGACCACTCACACCACAAAGTTGTGGTTTATATGCATCCATTCTAATCCACTCCACCCCGATTGGTGTGTCTCACACCAATCTTCTTCTTCTTCTTCTCTCTCTCTTATTTTTCCATGGCGGCAAAACTTTCCACCGCCACAACCTTAAAGCCCTACAATCTCATCCAAACATCAACTTCTTCCCTAAAACACCCCAACAATATTCTTCACCCCCTTAACACCCCCAACAATATTCTTCACCCCCTTAACAAGCTTTCGTCATAAAACACTCCTTCAAACCCATAGAAGATCAAGAACAACACCCTTCACCGTTTGTGTTCTGATGGAGGATCCAAAACACACAACCCAAATAGACACAGAAGAAAAAACAGAACTTCCTAATAATAAACCCAACCCTCAATTTTCAGTATCACAAAAACTGGCAAGAAAGAAGTCACAGAGGTCCACTTACCTTGTTGCCGCTGTAATGTCTAGCTTTGGTGTTACCTCTATGGCAATCTTAGCAGTTTATTATAGATTTTCTTGGCAAATGGAGGGTAGTGGAGAAGTTCCATGGTCTGAAATGTTTGGCACATTTGCCCTCTCAGTGGGTGCTGCTGTGGGTATGGAGTTTTGGGCAAGATGGGCTCATGAGGTTCTATGGCATGCTTCCTTGTGGCACATGCATGAGTCACATCATCGACCAAGAGAAGGTCCATTTGAGCTTAATGATGTTTTTGCAATAATTAACGCTGTCCCTGCAATTGCTCTTCTCTCATACGGTTTTTTCAACAAGGGATTGTTTCCTGGACTCTGTTTTGGTGCAGGTCTTGGAATTACAGTGTTTGGGATTGCTTACATGTTTGTCCATGATGGATTGGTTCATAAGAGATTCCCTGTGGGTCCTATTGCTAATGTGCCTTACTTTACAAGGGTTGCTGCTGCTCATCAACTTCATCATTCAGACAAATTCAAAGGAGTGCCATACGGGCTCTTTTTGGGCCCAAAGGAAGTTGAAGAGGTAGGAGGACTAGAAGAGCTGGAGAAAGAGATAAGTAGAAGAACAAGATCATATACTGGTTCATGACAAATGAATAGTATTATAGTTGTTTTAAAGAATTTTGAGTATGAAGAGCAATTCAATTCAATTAATGAAGTGATTGAATGTGAGTGTTAATTTGTCCAGTTATAGTTATTAATTTCAAGATAATCAAAGCCTGACATTAAACTGNAGTAGGTGTGGGTTCAAGG
TARGET=121,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1349
PRIMER_LEFT_PENALTY=0.111365
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=ACTGACCACTCACACCACAAAG
PRIMER_RIGHT_SEQUENCE=AGGGAAGAAGTTGATGTTTGGA
PRIMER_LEFT=23,22
PRIMER_RIGHT=210,22
PRIMER_LEFT_TM=60.111
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.1349
PRIMER_LEFT_1_PENALTY=0.111365
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=CTGACCACTCACACCACAAAGT
PRIMER_RIGHT_1_SEQUENCE=AGGGAAGAAGTTGATGTTTGGA
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=210,22
PRIMER_LEFT_1_TM=60.111
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.1455
PRIMER_LEFT_2_PENALTY=0.111365
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=CTGACCACTCACACCACAAAGT
PRIMER_RIGHT_2_SEQUENCE=TTGGATGAGATTGTAGGGCTTT
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=193,22
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.1455
PRIMER_LEFT_3_PENALTY=0.111365
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=CTGACCACTCACACCACAAAGT
PRIMER_RIGHT_3_SEQUENCE=TTTGGATGAGATTGTAGGGCTT
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=194,22
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.1455
PRIMER_LEFT_4_PENALTY=0.111365
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=ACTGACCACTCACACCACAAAG
PRIMER_RIGHT_4_SEQUENCE=TTTGGATGAGATTGTAGGGCTT
PRIMER_LEFT_4=23,22
PRIMER_RIGHT_4=194,22
PRIMER_LEFT_4_TM=60.111
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_03654 Ps_contig_mira-and-tgicl-ass_franssen_30630
SEQUENCE=ATTTCATTGTTTTGTTGTTTCACACTGACCACTCACACCACAAAGTTGTGGTTTATATGCATCCATTCTAATCCACTCCACCCCGATTGGTGTGTCTCACACCAATCTTCTTCTTCTTCTTCTCTCTCTCTTATTTTTCCATGGCGGCAAAACTTTCCACCGCCACAACCTTAAAGCCCTACAATCTCATCCAAACATCAACTTCTTCCCTAAAACACCCCAACAATATTCTTCACCCCCTTAACACCCCCAACAATATTCTTCACCCCCTTAACAAGCTTTCGTCATAAAACACTCCTTCAAACCCATAGAAGATCAAGAACAACACCCTTCACCGTTTGTGTTCTGATGGAGGATCCAAAACACACAACCCAAATAGACACAGAAGAAAAAACAGAACTTCCTAATAATAAACCCAACCCTCAATTTTCAGTATCACAAAAACTGGCAAGAAAGAAGTCACAGAGGTCCACTTACCTTGTTGCCGCTGTAATGTCTAGCTTTGGTGTTACCTCTATGGCAATCTTAGCAGTTTATTATAGATTTTCTTGGCAAATGGAGGGTAGTGGAGAAGTTCCATGGTCTGAAATGTTTGGCACATTTGCCCTCTCAGTGGGTGCTGCTGTGGGTATGGAGTTTTGGGCAAGATGGGCTCATGAGGTTCTATGGCATGCTTCCTTGTGGCACATGCATGAGTCACATCATCGACCAAGAGAAGGTCCATTTGAGCTTAATGATGTTTTTGCAATAATTAACGCTGTCCCTGCAATTGCTCTTCTCTCATACGGTTTTTTCAACAAGGGATTGTTTCCTGGACTCTGTTTTGGTGCAGGTCTTGGAATTACAGTGTTTGGGATTGCTTACATGTTTGTCCATGATGGATTGGTTCATAAGAGATTCCCTGTGGGTCCTATTGCTAATGTGCCTTACTTTACAAGGGTTGCTGCTGCTCATCAACTTCATCATTCAGACAAATTCAAAGGAGTGCCATACGGGCTCTTTTTGGGCCCAAAGGAAGTTGAAGAGGTAGGAGGACTAGAAGAGCTGGAGAAAGAGATAAGTAGAAGAACAAGATCATATACTGGTTCATGACAAATGAATAGTATTATAGTTGTTTTAAAGAATTTTGAGTATGAAGAGCAATTCAATTCAATTAATGAAGTGATTGAATGTGAGTGTTAATTTGTCCAGTTATAGTTATTAATTTCAAGATAATCAAAGCCTGACATTAAACTGNAGTAGGTGTGGGTTCAAGG
TARGET=1141,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9389
PRIMER_LEFT_PENALTY=0.360320
PRIMER_RIGHT_PENALTY=1.578557
PRIMER_LEFT_SEQUENCE=GACAAATTCAAAGGAGTGCCAT
PRIMER_RIGHT_SEQUENCE=TGAACCCACACCTACTNCAGTTT
PRIMER_LEFT=969,22
PRIMER_RIGHT=1252,23
PRIMER_LEFT_TM=60.360
PRIMER_RIGHT_TM=60.579
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.50
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=1.9421
PRIMER_LEFT_1_PENALTY=0.363508
PRIMER_RIGHT_1_PENALTY=1.578557
PRIMER_LEFT_1_SEQUENCE=AAATTCAAAGGAGTGCCATACG
PRIMER_RIGHT_1_SEQUENCE=TGAACCCACACCTACTNCAGTTT
PRIMER_LEFT_1=972,22
PRIMER_RIGHT_1=1252,23
PRIMER_LEFT_1_TM=60.364
PRIMER_RIGHT_1_TM=60.579
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.50
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=2.1300
PRIMER_LEFT_2_PENALTY=0.360320
PRIMER_RIGHT_2_PENALTY=1.769631
PRIMER_LEFT_2_SEQUENCE=GACAAATTCAAAGGAGTGCCAT
PRIMER_RIGHT_2_SEQUENCE=CCTTGAACCCACACCTACTNC
PRIMER_LEFT_2=969,22
PRIMER_RIGHT_2=1255,21
PRIMER_LEFT_2_TM=60.360
PRIMER_RIGHT_2_TM=59.230
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7500
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=2.1331
PRIMER_LEFT_3_PENALTY=0.363508
PRIMER_RIGHT_3_PENALTY=1.769631
PRIMER_LEFT_3_SEQUENCE=AAATTCAAAGGAGTGCCATACG
PRIMER_RIGHT_3_SEQUENCE=CCTTGAACCCACACCTACTNC
PRIMER_LEFT_3=972,22
PRIMER_RIGHT_3=1255,21
PRIMER_LEFT_3_TM=60.364
PRIMER_RIGHT_3_TM=59.230
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.7500
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=2.2194
PRIMER_LEFT_4_PENALTY=0.640802
PRIMER_RIGHT_4_PENALTY=1.578557
PRIMER_LEFT_4_SEQUENCE=TCAGACAAATTCAAAGGAGTGC
PRIMER_RIGHT_4_SEQUENCE=TGAACCCACACCTACTNCAGTTT
PRIMER_LEFT_4=966,22
PRIMER_RIGHT_4=1252,23
PRIMER_LEFT_4_TM=59.359
PRIMER_RIGHT_4_TM=60.579
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.50
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_03655 Ps_contig_mira-and-tgicl-ass_franssen_30631
SEQUENCE=ACTAAAATTGAAATAATAATATATATATCCTCTAAAAAAGAGGCTAAAAAAAGCACGAGGCACAAGTAATAATTAGAAAAATAAAAGAGGAATGATTTGTGGAACCAATACAAGTAAAATGTAAACTGTATTAACAGAAGAAACCTTTTAATGGGTAAAGCTTTGTCAGGAAATCGACCCCTTAAGCATCTTTTTTATATACTTGTAGGGTCAATTCCCAACTTGTTGTAGCTTTCCTCAATAAAATGAACGACAGACAACAATCCACTCAATAAAAAGTAAAAGACTTTTTAGAATTTTACCACCAAGTAACGTGATCGAGTGTCGGCTGCTAATACTGTACCTTCTCGTCATTGAAATTCATGCTCTCAATACCTCGGAGAGTTGACTCAAAATTCTTTATGTTTGATGTGTTTCTGCTGGATGTTCCTTTGAGCTCCGTTGATGTGATAGGTGAATTTCTTTGAGCACCAGAGGTTTTAAGGAGTGATCCAGGACTACCATCCCTTGCAAGAGGAAGAGAGGGTTCAATTTCACTTCCAACAACTGCATCTCGACTGTTGGAAACAGCGCCTACTCGTCTTGTGGATCCACTTGGTCGGAAGAAATTAGAACTAGACAACATAGCATCTTTAGATGCAGTTGAGTCACTTGGAACCGGGGCTTTTTGTCTAAATAAGTTTACATCATTTGCAATAGGTCCAGAGTTTCTTCTACGAGCAGGATCTGATGAAGACCAACCAGCATGTCTACCCTCTTCCCCACCTGTCGGTCTATCAGTAGCACTCACAACAGCTGGTGGTATGCCAGAACTTGGCCCAGCACCAGAACCAATGGCACGTGAAGGAGGAGTGGCAATTTGAGATTGCTGATACTTCAAGATGGTCCAATCAAAGACATAATCAAACTGGAACCCTTCACGAATGAAAAGGTCACGAAAAAGCCTTTTCAAATATGCATAATCTGGTTTATCATCAAACCGCAATGACCGACAGTAATGGAAGTACGAAGCAAATTCTGAGGGATAGCCACGGCATAAGGACTCAATAGAAGTAGAAACTTTCTTCTCACTGATTTTTTCGTACTTCTGTTTCTTAGTACCTGCTTTCAATCCCTGCCATGGAAGACTTCCTCTCAAAAAGTACATAAGAACATATCCAAGTGATTCTAAGTCATCCCTACGGCTTTGCTCAATTCCAAGATGTGTATTCATACTAGCATATCTAGCAGTTCCTGTCAAATTCTTATTCTCTCTGTAAGGAATATGCTGATGGGTAGAGGTGTCTCTGTATTTTTTAGCGAGACCAAAGTCAATGGCATAAACTTGATTAGCACGCCTCCCCAAACCCATCAGGAAGTTGTCTGGCTTGATGTCCCTATGTAAAAATGATTTGGAATGAATATATTCAACACGATTTATCATCTGATCAGCAAGCATGAGTACAGTTTTAAGGGACAATTTGCGACTACAAAAACTGAATAAATCTTCGAGACTAGGTCCTAGTAAATCCATCACAAGAACATTGTACTCTCCTTCAACGCCAAACCATCTCACATTTGGGATACCAGTTCCTCCTTGAAGTACTTTATACAACTTTGATTCATACAACAATTGAGGATGCTTGGTTTTGACATTTTCAAGCTTGATTGCAACCTCTTCATTTGTCTGAATATTAGTACCGAGATAGATCTCGCCGAAGGATCCGCTGCCAATCTTGCGGCCAAGACGGAATTTGTTCCCAATACGAGGCTCCATTCGATCGGGTCAAAACCCTAGATGCGAGAATAAAACCCTAGAAATTGGGAAATTGAGAATCGGAAATTGAAAATGGATGGAGTTGGAAGTCGGTGTAGCTGAAGAGAATTGGAAGGGGAAAGGGAATGGGATAGAGACGGGAATGAAGAAAAGTTGAAGGACAAAAATGGAAGAAAAAAGTTTCTCTCTCTGAAATGAAGGTGATTATTAGGGTAGAGAGAGAGAGGGATCGTGATGATGCCTCTCCTCCCCTCCGCNTNCT
TARGET=19,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03656 Ps_contig_mira-and-tgicl-ass_franssen_30631
SEQUENCE=ACTAAAATTGAAATAATAATATATATATCCTCTAAAAAAGAGGCTAAAAAAAGCACGAGGCACAAGTAATAATTAGAAAAATAAAAGAGGAATGATTTGTGGAACCAATACAAGTAAAATGTAAACTGTATTAACAGAAGAAACCTTTTAATGGGTAAAGCTTTGTCAGGAAATCGACCCCTTAAGCATCTTTTTTATATACTTGTAGGGTCAATTCCCAACTTGTTGTAGCTTTCCTCAATAAAATGAACGACAGACAACAATCCACTCAATAAAAAGTAAAAGACTTTTTAGAATTTTACCACCAAGTAACGTGATCGAGTGTCGGCTGCTAATACTGTACCTTCTCGTCATTGAAATTCATGCTCTCAATACCTCGGAGAGTTGACTCAAAATTCTTTATGTTTGATGTGTTTCTGCTGGATGTTCCTTTGAGCTCCGTTGATGTGATAGGTGAATTTCTTTGAGCACCAGAGGTTTTAAGGAGTGATCCAGGACTACCATCCCTTGCAAGAGGAAGAGAGGGTTCAATTTCACTTCCAACAACTGCATCTCGACTGTTGGAAACAGCGCCTACTCGTCTTGTGGATCCACTTGGTCGGAAGAAATTAGAACTAGACAACATAGCATCTTTAGATGCAGTTGAGTCACTTGGAACCGGGGCTTTTTGTCTAAATAAGTTTACATCATTTGCAATAGGTCCAGAGTTTCTTCTACGAGCAGGATCTGATGAAGACCAACCAGCATGTCTACCCTCTTCCCCACCTGTCGGTCTATCAGTAGCACTCACAACAGCTGGTGGTATGCCAGAACTTGGCCCAGCACCAGAACCAATGGCACGTGAAGGAGGAGTGGCAATTTGAGATTGCTGATACTTCAAGATGGTCCAATCAAAGACATAATCAAACTGGAACCCTTCACGAATGAAAAGGTCACGAAAAAGCCTTTTCAAATATGCATAATCTGGTTTATCATCAAACCGCAATGACCGACAGTAATGGAAGTACGAAGCAAATTCTGAGGGATAGCCACGGCATAAGGACTCAATAGAAGTAGAAACTTTCTTCTCACTGATTTTTTCGTACTTCTGTTTCTTAGTACCTGCTTTCAATCCCTGCCATGGAAGACTTCCTCTCAAAAAGTACATAAGAACATATCCAAGTGATTCTAAGTCATCCCTACGGCTTTGCTCAATTCCAAGATGTGTATTCATACTAGCATATCTAGCAGTTCCTGTCAAATTCTTATTCTCTCTGTAAGGAATATGCTGATGGGTAGAGGTGTCTCTGTATTTTTTAGCGAGACCAAAGTCAATGGCATAAACTTGATTAGCACGCCTCCCCAAACCCATCAGGAAGTTGTCTGGCTTGATGTCCCTATGTAAAAATGATTTGGAATGAATATATTCAACACGATTTATCATCTGATCAGCAAGCATGAGTACAGTTTTAAGGGACAATTTGCGACTACAAAAACTGAATAAATCTTCGAGACTAGGTCCTAGTAAATCCATCACAAGAACATTGTACTCTCCTTCAACGCCAAACCATCTCACATTTGGGATACCAGTTCCTCCTTGAAGTACTTTATACAACTTTGATTCATACAACAATTGAGGATGCTTGGTTTTGACATTTTCAAGCTTGATTGCAACCTCTTCATTTGTCTGAATATTAGTACCGAGATAGATCTCGCCGAAGGATCCGCTGCCAATCTTGCGGCCAAGACGGAATTTGTTCCCAATACGAGGCTCCATTCGATCGGGTCAAAACCCTAGATGCGAGAATAAAACCCTAGAAATTGGGAAATTGAGAATCGGAAATTGAAAATGGATGGAGTTGGAAGTCGGTGTAGCTGAAGAGAATTGGAAGGGGAAAGGGAATGGGATAGAGACGGGAATGAAGAAAAGTTGAAGGACAAAAATGGAAGAAAAAAGTTTCTCTCTCTGAAATGAAGGTGATTATTAGGGTAGAGAGAGAGAGGGATCGTGATGATGCCTCTCCTCCCCTCCGCNTNCT
TARGET=1971,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.4438
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=3.348388
PRIMER_LEFT_SEQUENCE=TCAAAACCCTAGATGCGAGAAT
PRIMER_RIGHT_SEQUENCE=GAGGAGAGGCATCATCACGA
PRIMER_LEFT=1765,22
PRIMER_RIGHT=2004,20
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=61.348
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=3.4505
PRIMER_LEFT_1_PENALTY=0.102139
PRIMER_RIGHT_1_PENALTY=3.348388
PRIMER_LEFT_1_SEQUENCE=CGGGAATGAAGAAAAGTTGAAG
PRIMER_RIGHT_1_SEQUENCE=GAGGAGAGGCATCATCACGA
PRIMER_LEFT_1=1893,22
PRIMER_RIGHT_1=2004,20
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=61.348
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=112
PRIMER_PAIR_PENALTY_2=3.4979
PRIMER_LEFT_2_PENALTY=0.149516
PRIMER_RIGHT_2_PENALTY=3.348388
PRIMER_LEFT_2_SEQUENCE=TAGCTGAAGAGAATTGGAAGGG
PRIMER_RIGHT_2_SEQUENCE=GAGGAGAGGCATCATCACGA
PRIMER_LEFT_2=1851,22
PRIMER_RIGHT_2=2004,20
PRIMER_LEFT_2_TM=59.850
PRIMER_RIGHT_2_TM=61.348
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=3.4979
PRIMER_LEFT_3_PENALTY=0.149559
PRIMER_RIGHT_3_PENALTY=3.348388
PRIMER_LEFT_3_SEQUENCE=GGAAAGGGAATGGGATAGAGAC
PRIMER_RIGHT_3_SEQUENCE=GAGGAGAGGCATCATCACGA
PRIMER_LEFT_3=1872,22
PRIMER_RIGHT_3=2004,20
PRIMER_LEFT_3_TM=60.150
PRIMER_RIGHT_3_TM=61.348
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=3.7170
PRIMER_LEFT_4_PENALTY=0.368624
PRIMER_RIGHT_4_PENALTY=3.348388
PRIMER_LEFT_4_SEQUENCE=AGACGGGAATGAAGAAAAGTTG
PRIMER_RIGHT_4_SEQUENCE=GAGGAGAGGCATCATCACGA
PRIMER_LEFT_4=1890,22
PRIMER_RIGHT_4=2004,20
PRIMER_LEFT_4_TM=59.631
PRIMER_RIGHT_4_TM=61.348
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_03657 Ps_contig_mira-and-tgicl-ass_franssen_30638
SEQUENCE=GGTTCTAGAAACCGGAAAATTTGATCAATTCAATACAATGAACTGGTTAGATGGTTTGGTGGATAGAAAGAGTTAATAAACAAACAATATTATACTCTGCTCCTTCTTCACCCCTTCAACCATCTCACCAGTCCTTTGGCAGAACCACCATAACCACCGCTATCAATGGGTAGAGCCGCTCTCATAACGACGGCGGTAGTTTTCCTAGCCGTCGGAGTAATTTTCTCTCCGGAAACATTCGGATCAAAATCAACCACTCTTTCCACTTACCTCAAACTAGCCCACCTCCTGGGTTTCTCCATCGCTTTCGGCGCCGCTCTATGGGTAACCTTTATCGGCGGGATCATCATGTTCAAGAATTTGCCTAGGCATCAGTTTGGTAATCTTCAAAGCAAGATGTTTCCGGCTTATTTCTCCATGGTTGGTGTTTGTATCTCTGTCGCAGTGGCTTCCTTTGGATACCTTCACCCATGGAAAACTTCATCTACTAGTGAGAAATACCAACTTGGCTTCTTGCTCTCTGCTCTTGCGTTCAATCTTACTAATTTGTTCGTCTTTACGCCAATGACAATTGAGATGATGAAACAAAGGCATAAAGTGGAGAGAGAGAATAAGATTGGAGATGAAGTTGGGTGGTCAAAAAATGTTGAAGTTGCAAAGAAAAACCCAAAGCTTGCAGCGATGAACAAGAAGTTTGGTATGATTCATGGACTATCTTCCCTTGCCAATATCTTGTCATTTGGAAGCCTTGCAATACACTCTTGGTACTTGGCAGGTAAACTTGATCTCTGATTCAGTTTTTGCCTCCCTTTCTAGTAGACGAGAAACTTGTCCATGCAATGCAATTTGCGAAATGTACTAGCAAAACATTACACAGTATTCGTTTATCAAGAGTTCTTGTGTCTTTGTGTGAAAGAAAACCTAATGTAATTGCTTTAATTCTCACATGAATAAAATTAGTCAGTGTACCTTTCTGCAGTTGAAGGTAACTTATATTATGGTATAAATTCTATAAAGCACCAATTTGCAANNCGGT
TARGET=601,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2450
PRIMER_LEFT_PENALTY=0.210886
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=ATACCAACTTGGCTTCTTGCTC
PRIMER_RIGHT_SEQUENCE=ACAAGATATTGGCAAGGGAAGA
PRIMER_LEFT=497,22
PRIMER_RIGHT=735,22
PRIMER_LEFT_TM=59.789
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.2450
PRIMER_LEFT_1_PENALTY=0.210886
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=ATACCAACTTGGCTTCTTGCTC
PRIMER_RIGHT_1_SEQUENCE=GACAAGATATTGGCAAGGGAAG
PRIMER_LEFT_1=497,22
PRIMER_RIGHT_1=736,22
PRIMER_LEFT_1_TM=59.789
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=0.2475
PRIMER_LEFT_2_PENALTY=0.151911
PRIMER_RIGHT_2_PENALTY=0.095629
PRIMER_LEFT_2_SEQUENCE=TCTGCTCTTGCGTTCAATCTTA
PRIMER_RIGHT_2_SEQUENCE=ACCTGCCAAGTACCAAGAGTGT
PRIMER_LEFT_2=519,22
PRIMER_RIGHT_2=776,22
PRIMER_LEFT_2_TM=60.152
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.2551
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=CTTCACCCATGGAAAACTTCAT
PRIMER_RIGHT_3_SEQUENCE=ACAAGATATTGGCAAGGGAAGA
PRIMER_LEFT_3=462,22
PRIMER_RIGHT_3=735,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.2551
PRIMER_LEFT_4_PENALTY=0.220992
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=CTTCACCCATGGAAAACTTCAT
PRIMER_RIGHT_4_SEQUENCE=GACAAGATATTGGCAAGGGAAG
PRIMER_LEFT_4=462,22
PRIMER_RIGHT_4=736,22
PRIMER_LEFT_4_TM=60.221
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_03658 Ps_contig_mira-and-tgicl-ass_franssen_30641
SEQUENCE=CAACNYNTCGNGCAATTCTGAGTGTGACTGTGACTTAATCAAAATAATTCAATTAGAAACTATAGTGACTGTGATTGAATTTGACATATGACAATGGTGAATGACTAAGTGGAAGTTCTAACGACCACACATTCATAAAATTTTAACCTATAGTTTCTCCGATTGATTCGATTCTCTTTTTTTTTCACTCTCTCTCTGTTCTTAACTTTGGAAATGGCATTCCCAATTCCACACCTTATCTCTTCACCAGCATCTTCCTTTCTACCGTACAACCCTTTGTTAAACCTCACTCCACTTTCCTTAAACCCTAAAATCCCATTCTCAACACCTACGCCGCCGCATATTAACGTTCTCCGCCACATTCGCCGCCGTAAGGCTACTTCACACCTCCGATGCTCGGCGTCGTCTTACCCGGAGAACAACAACGCTAACTCTCCTAATCAAGATGACGTAACGGAGCTACCTCTGTTTCCTCTCCCTTTGGTTCTCTTCCCCGGAGCAATCCTCCCTCTCCAGATCTTCGAGTTTCGCTACCGTGTTATGATGCATACGCTCCTACATACGGATCTCCGATTCGGTGTTATCTACACCGACGCGGTTACTGGAACGGCGGAAGTCGGTTGCGTTGGAGAAGTTATAAAACATGAGAGACTTGTCGATGATCGATTCTTCTTGATCTGTAAAGGTCAGGAGCGTTTCCGTGTGAAGAAAGTGGTTCGGACGAAACCCTACCTTGTGGCGAGTGTGGCGTGGTTGGAGGATCGACCTTCTCCGTCTGCTGACGTGGATGTTGACGGTTTGGCAAACGAAGTGGAGACGTATATGAAAGATGTTATTCGGTTATCGAATCGGTTAGGTGGAAAGGCGGAGAAAGAGGTTGGTGATTTGAGGAGGAACTTGTTTCCGACGCCGTTTTCTTTCTTTGTTGGAAGCACGTTTGAAGGCGCGCCGAGAGAGCAACAGGCTTTGTTGGAATTGGAAGATACTGCTGCGAGATTGGCGAGGGAGAAGGAAACTTTGAAGAATACACTGAATTACTTATCTGCTGCTTCTGCTGTCAAAGATGTTTTTCCATCTTCTTCGTCTCCTTCGTCTCCCTCTTGAGAGGTATGATTTGTACTGTATCCACAATAGGCAATAGCACCAATGTATTATACTCAGTTGTAAATTCTCGTAAACTTTTCATATGTTGATCATGTCTGGTTATTCAATTTTTGGATGACATTTTATTTCATAATATACAATGATGGTGATACTTGATAGTAATCTTTTCTAAAAATAGGTAGTTGTTTAATCGG
TARGET=188,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03659 Ps_contig_mira-and-tgicl-ass_franssen_30642
SEQUENCE=ATAATCCAGCTCTTTTGATTTGCATTGGNNAGCAAACATAAGACAAGAGAGAGCGAAGATTCCCTTGTTCCTTCCACCGAGCGAGCATCAGCAGTGTAAAATAAAAGAATAAATTTTTTTACTTGAGAGTTGGAATCGAAGACGCAGAAGAAAACATTCATGTGAGAGAAAGGAATAGGAAAGTGTCGTGGGAGGAAGAAAGTGTTTTAAAAACGCCCAAAGCAAAAGCTCCTCCCTCCCTCCCTCCGATTATTCAGATCTACTCCCCTCCCTCGTAGAAGAAGAAGAAGATGGAGAGCGAAAAGAAATCCTCTTCCATTTCAAACTTTGGAGCATGGGGTATGAACGTTGTGAGCTCCGTTGGAATTATCATGGCTAATAAACACCTCATGTCCAATAATGGCTACGCTTTCACCTTCGCCACTACATTGACCGGCTTCCACTTCGCCGTTACTGCTCTCGTCGGTCTTGTCTCCAACGCTTCCGGTTATTCTGCTCCCAAACACGTCCCTCTTTGGGAGCTTCTTTGGTTCTCAGCTATTGCCAATATCTCTATCACAGGGATGAACCTCAGCCTCATGCTCAATTCTGTTGGTTTCTACCAAATATCAAAACTAAGCATGATTCCCGTCGTTTGTATTATGGAATGGATCCTTCACGGCAAACACTACTCCAGGGAAGTCAAAACGGCTGTCATGGTTGTCGTTATTGGTGTCGGCGTTTGCACTGTAACGGATGTCAAAGTTAACCTCAAAGGTTTCGTATGTGCCTGTCTAGCCGTTGTATCATCATCTTTACAGCAAATTTCAATTGGATCTCTACAAAAGAAGTATTCAATTGGATCTTTTGAATTGCTAAGTAAGACTGCTCCTATTCAAGCCCTCTCTCTAATAATTCTTGGTCCATTTGTTGATTACTATCTCAGTGGGAAGTTGATAACCAACTTTAAGTTGTCTTCCGGTGCCATTATATTCATTCTTCTTTCATGCACCCTAGCTGTGTTTTGCAATATAAGCCAGTACCTCTGCATTGGACGTTTCTCAGCAGTTTCCTTCCAGGTTTTAGGCCACATGAAAACAGTATGTGTTCTGACTTTGGGGTGGCTACTATTCGATTCAGAGCTAACTTTCAAGAACATCATGGGAATGATTCTTGCAGTTGTTGGGATGGTAATTTATAGTTGGGCTGTCGAGCTTGAAAAGTCGTCCAGTGCAAAGAGTCTTCCTCATGCAAAAAACAGCCTAACAGAAGAAGAAATTAGACTATTGAGAGAAGGGATAGAAAACAGTACGTTGAAAGATGTTGAACTTGGTGAGGCTAAAGTTTAGATTGATTGGAAGTGATTAGTGTCAATTTTAATGTGGATTACTTCAATTCTATTTGTTCATTTTATAGTTTACTTGCTAAAAGACTTGGTGTCTATCTGTTTCTACGAACTAATCCATTTTAGAAAATGTATCTTATTTTTTAATTTTGATTGTTTTTGCTGAGATTGTGTTATTTATTTGGCTTGTCGGTACAAGACTGCCAGGCGCTATTGACATGTAAAATGTGTAAGCTCATGACAGACAATATATTCTGTCAATTGTCTTTTATGTTAGATATCAAATTTTCATTANNTTATTTTTAC
TARGET=231,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0285
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.026163
PRIMER_LEFT_SEQUENCE=GAATAGGAAAGTGTCGTGGGAG
PRIMER_RIGHT_SEQUENCE=CCAAAGTTTGAAATGGAAGAGG
PRIMER_LEFT=172,22
PRIMER_RIGHT=330,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.974
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=159
PRIMER_PAIR_PENALTY_1=0.0364
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=GAATAGGAAAGTGTCGTGGGAG
PRIMER_RIGHT_1_SEQUENCE=TTTCTTTTCGCTCTCCATCTTC
PRIMER_LEFT_1=172,22
PRIMER_RIGHT_1=307,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.1112
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.108929
PRIMER_LEFT_2_SEQUENCE=GAATAGGAAAGTGTCGTGGGAG
PRIMER_RIGHT_2_SEQUENCE=GCTCCAAAGTTTGAAATGGAAG
PRIMER_LEFT_2=172,22
PRIMER_RIGHT_2=333,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=0.1122
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.109882
PRIMER_LEFT_3_SEQUENCE=GAATAGGAAAGTGTCGTGGGAG
PRIMER_RIGHT_3_SEQUENCE=AAGCGTAGCCATTATTGGACAT
PRIMER_LEFT_3=172,22
PRIMER_RIGHT_3=410,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=59.890
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=0.1278
PRIMER_LEFT_4_PENALTY=0.101680
PRIMER_RIGHT_4_PENALTY=0.026163
PRIMER_LEFT_4_SEQUENCE=AATCGAAGACGCAGAAGAAAAC
PRIMER_RIGHT_4_SEQUENCE=CCAAAGTTTGAAATGGAAGAGG
PRIMER_LEFT_4=133,22
PRIMER_RIGHT_4=330,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=59.974
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=198
=
PRIMER_SEQUENCE_ID=SSR_03660 Ps_contig_mira-and-tgicl-ass_franssen_30642
SEQUENCE=ATAATCCAGCTCTTTTGATTTGCATTGGNNAGCAAACATAAGACAAGAGAGAGCGAAGATTCCCTTGTTCCTTCCACCGAGCGAGCATCAGCAGTGTAAAATAAAAGAATAAATTTTTTTACTTGAGAGTTGGAATCGAAGACGCAGAAGAAAACATTCATGTGAGAGAAAGGAATAGGAAAGTGTCGTGGGAGGAAGAAAGTGTTTTAAAAACGCCCAAAGCAAAAGCTCCTCCCTCCCTCCCTCCGATTATTCAGATCTACTCCCCTCCCTCGTAGAAGAAGAAGAAGATGGAGAGCGAAAAGAAATCCTCTTCCATTTCAAACTTTGGAGCATGGGGTATGAACGTTGTGAGCTCCGTTGGAATTATCATGGCTAATAAACACCTCATGTCCAATAATGGCTACGCTTTCACCTTCGCCACTACATTGACCGGCTTCCACTTCGCCGTTACTGCTCTCGTCGGTCTTGTCTCCAACGCTTCCGGTTATTCTGCTCCCAAACACGTCCCTCTTTGGGAGCTTCTTTGGTTCTCAGCTATTGCCAATATCTCTATCACAGGGATGAACCTCAGCCTCATGCTCAATTCTGTTGGTTTCTACCAAATATCAAAACTAAGCATGATTCCCGTCGTTTGTATTATGGAATGGATCCTTCACGGCAAACACTACTCCAGGGAAGTCAAAACGGCTGTCATGGTTGTCGTTATTGGTGTCGGCGTTTGCACTGTAACGGATGTCAAAGTTAACCTCAAAGGTTTCGTATGTGCCTGTCTAGCCGTTGTATCATCATCTTTACAGCAAATTTCAATTGGATCTCTACAAAAGAAGTATTCAATTGGATCTTTTGAATTGCTAAGTAAGACTGCTCCTATTCAAGCCCTCTCTCTAATAATTCTTGGTCCATTTGTTGATTACTATCTCAGTGGGAAGTTGATAACCAACTTTAAGTTGTCTTCCGGTGCCATTATATTCATTCTTCTTTCATGCACCCTAGCTGTGTTTTGCAATATAAGCCAGTACCTCTGCATTGGACGTTTCTCAGCAGTTTCCTTCCAGGTTTTAGGCCACATGAAAACAGTATGTGTTCTGACTTTGGGGTGGCTACTATTCGATTCAGAGCTAACTTTCAAGAACATCATGGGAATGATTCTTGCAGTTGTTGGGATGGTAATTTATAGTTGGGCTGTCGAGCTTGAAAAGTCGTCCAGTGCAAAGAGTCTTCCTCATGCAAAAAACAGCCTAACAGAAGAAGAAATTAGACTATTGAGAGAAGGGATAGAAAACAGTACGTTGAAAGATGTTGAACTTGGTGAGGCTAAAGTTTAGATTGATTGGAAGTGATTAGTGTCAATTTTAATGTGGATTACTTCAATTCTATTTGTTCATTTTATAGTTTACTTGCTAAAAGACTTGGTGTCTATCTGTTTCTACGAACTAATCCATTTTAGAAAATGTATCTTATTTTTTAATTTTGATTGTTTTTGCTGAGATTGTGTTATTTATTTGGCTTGTCGGTACAAGACTGCCAGGCGCTATTGACATGTAAAATGTGTAAGCTCATGACAGACAATATATTCTGTCAATTGTCTTTTATGTTAGATATCAAATTTTCATTANNTTATTTTTAC
TARGET=277,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0285
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.026163
PRIMER_LEFT_SEQUENCE=GAATAGGAAAGTGTCGTGGGAG
PRIMER_RIGHT_SEQUENCE=CCAAAGTTTGAAATGGAAGAGG
PRIMER_LEFT=172,22
PRIMER_RIGHT=330,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.974
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=159
PRIMER_PAIR_PENALTY_1=0.1112
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=GAATAGGAAAGTGTCGTGGGAG
PRIMER_RIGHT_1_SEQUENCE=GCTCCAAAGTTTGAAATGGAAG
PRIMER_LEFT_1=172,22
PRIMER_RIGHT_1=333,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.1122
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.109882
PRIMER_LEFT_2_SEQUENCE=GAATAGGAAAGTGTCGTGGGAG
PRIMER_RIGHT_2_SEQUENCE=AAGCGTAGCCATTATTGGACAT
PRIMER_LEFT_2=172,22
PRIMER_RIGHT_2=410,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=59.890
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.1278
PRIMER_LEFT_3_PENALTY=0.101680
PRIMER_RIGHT_3_PENALTY=0.026163
PRIMER_LEFT_3_SEQUENCE=AATCGAAGACGCAGAAGAAAAC
PRIMER_RIGHT_3_SEQUENCE=CCAAAGTTTGAAATGGAAGAGG
PRIMER_LEFT_3=133,22
PRIMER_RIGHT_3=330,22
PRIMER_LEFT_3_TM=59.898
PRIMER_RIGHT_3_TM=59.974
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=0.2106
PRIMER_LEFT_4_PENALTY=0.101680
PRIMER_RIGHT_4_PENALTY=0.108929
PRIMER_LEFT_4_SEQUENCE=AATCGAAGACGCAGAAGAAAAC
PRIMER_RIGHT_4_SEQUENCE=GCTCCAAAGTTTGAAATGGAAG
PRIMER_LEFT_4=133,22
PRIMER_RIGHT_4=333,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=60.109
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=201
=
PRIMER_SEQUENCE_ID=SSR_03661 Ps_contig_mira-and-tgicl-ass_franssen_30645
SEQUENCE=CGACGCCCTGATGCAAAAGAAAGTACAGAACAGCATTTTTTGTCCAAAAATAAATGCAGGTGCTTTGTATTTCTCAAACATAAGCTCTGCTGCCCTTTCTCTCTGTTGTGGAGCATTGGAAGAAGGTTCTGCCAGGAGCATGGGATGTTCTTTAGGATCAATCAATAAGCACTCCCTACTGAAGGCATGATCCCAAATGCTGTCAACAATATCCCAGTCAGCAACAAGTCCATCCTTCAGTGGAGACAGTACCTCCATAAAATCTCTACGGTAGCCCAAGGACTGAGATCCTACATACAATTTTCTCTTTCCCTTGGCTTTATCACCCTCAACATTTCTACTATTGTTCTTGGATTCCCCAGAGCCAGAGTTCTTTTCAGGATCATCCGATTCATCAACATCCATTTGATCAATAGCTCCAACAACAGAAGGAAACACGGCCTTGGGAGCATCTTCTCCGGCATAACCAGCTTTACAGGTATGTGAACCCAGATCAATCACTATAGCYGAAACCTCGTCACCGCCGTACATGACTGTAACAGTCCCGGAAACTCGACGGGAGAATTAGGGTTTATTAATAAGTTATGAATGTTAGGGTTTTAGAAATTTCTCTTTCTATCGCTTCAAGAGAGAGAGGTGGGGGAGGGGTGAGTTCCGATGACGAACTACGCCGCCGCGGTAGTCAACGGCGAAGGAGGAGGAGAAGCGCGCGCGATTTNAAGAAGAAGAAAAAGAAAAGCTAGGGTATATGCGTTTTATTGG
TARGET=627,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03662 Ps_contig_mira-and-tgicl-ass_franssen_30646
SEQUENCE=TTTTTTTTACAAAATTAATGCAAGCAAACAACTTCTTGCATAATAAATAAATAAAATGTGATAATCACAATAGCAAACACCATTTGATCCTTTATATTATTGACAATGAATGCTAAAATGGACTAGCAAGGGTTATGAATCGATGTGCATTTTGAAGGAACCCCTCCAAATGCCATCAGATAGACAACAAATATTTGAATGTTTGTCTGTTCCTTGTCTTTGGTGGCAAAAATTTAGTGACACAAAATCATAATCAACAAATTCTTGCTGACTCAAAAAATCAAAGATGGTCAATAATCGCTTTTGTAAATTCGGTTGTCTTTGAAATGCCGCCAAGATCGGCAGTTCGGTACTTCCCTTCTGCAATTGTGTTGAGGATTGCATTTTGAATCCGATCTGCGTTGTCATGGAGATCCAAATGGCGCAACATTGAGACAGAACTCAGCAGCAAAGCTGTTGGATTTGCCAAATTCTTTCCAGCGATATCAGGTGCTGAACCATGTACAGCCTCAGCAAGTGCAATGCCTCCCCCACCAATGTTAATGCTTGGTGTCAAGCCCAATCCCCCAATCAAACCAGCACAGAGATCGCTGATTATATCGCCATACAGGTTAGGCATCACCAGTACATCGAAAAGTGCAGGATTCTTCACAAGCATCATGCAGCAATTGTCAATGACAACTTCCTCATACTTTATATCAGGATACTTCTCTGCAACTTCACGGCAACACTTGAGGAAAAGACCATCCGTCTTTTGCATAATATTGGCTTTGTGTATAGCAGACACCCTCTTTCTTCCATGTGCTTTGGCGTAGTGAAAAGCATACTCAGCCACCCTTGTACTTGCTTGTCGTGTAATAATTTTAATACTTTCTACTACACCCCTCACAACCTGATGTTCAAGTCCACTATACTCGCCTTCTGTATTTTCACGGATTGTAACGAGATCAACATTATCATATCGAGTTTTGTATCCAGAAAGGCTGTAGCAAGGTCGAACATTGGCATATAAATTTAGTTCTTTTCTCAGAGTAAGGTTTAATGAACGATGCCCTTTTCCAATAGGGGTGGCCATTGGCCCTTTCAAGCCAACCTTATTCCGCCGAACTGATTCCAGACTTTCCCATGTCAGAAAGCTCTGGGTTCTAGGGTCGACTTCGGTCCCTACATAGTGCTCTTCCCATTCTATTGGTGCTTCAGCAGCTTGGAATATCTGTTTGACGGAATCGGCGATCTCGGGACCAATACCATCGCCGGGGAAAAGAGTGGCGCGGATCGGAGTTGAAACGGAAGAGAAGGGCCTATGGTTTGCGAAATAGCGGCTTCCGAGTGTTCGTCTGAGGATCTGAGAAGCCATACTTTATCACGCTATGTTCCTGATTCNGACATGCAACGCAGAA
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.3442
PRIMER_LEFT_PENALTY=4.304027
PRIMER_RIGHT_PENALTY=0.040146
PRIMER_LEFT_SEQUENCE=TGCAAGCAAACAACTTCTTG
PRIMER_RIGHT_SEQUENCE=TTGTCTATCTGATGGCATTTGG
PRIMER_LEFT=18,20
PRIMER_RIGHT=186,22
PRIMER_LEFT_TM=57.696
PRIMER_RIGHT_TM=59.960
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=4.4416
PRIMER_LEFT_1_PENALTY=4.304027
PRIMER_RIGHT_1_PENALTY=0.137550
PRIMER_LEFT_1_SEQUENCE=TGCAAGCAAACAACTTCTTG
PRIMER_RIGHT_1_SEQUENCE=ACATCGATTCATAACCCTTGCT
PRIMER_LEFT_1=18,20
PRIMER_RIGHT_1=145,22
PRIMER_LEFT_1_TM=57.696
PRIMER_RIGHT_1_TM=59.862
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=128
PRIMER_PAIR_PENALTY_2=4.4893
PRIMER_LEFT_2_PENALTY=4.304027
PRIMER_RIGHT_2_PENALTY=0.185267
PRIMER_LEFT_2_SEQUENCE=TGCAAGCAAACAACTTCTTG
PRIMER_RIGHT_2_SEQUENCE=CACCAAAGACAAGGAACAGACA
PRIMER_LEFT_2=18,20
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=57.696
PRIMER_RIGHT_2_TM=60.185
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=4.7729
PRIMER_LEFT_3_PENALTY=4.304027
PRIMER_RIGHT_3_PENALTY=0.468913
PRIMER_LEFT_3_SEQUENCE=TGCAAGCAAACAACTTCTTG
PRIMER_RIGHT_3_SEQUENCE=TGTCTATCTGATGGCATTTGGA
PRIMER_LEFT_3=18,20
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=57.696
PRIMER_RIGHT_3_TM=60.469
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=4.8946
PRIMER_LEFT_4_PENALTY=4.304027
PRIMER_RIGHT_4_PENALTY=0.590581
PRIMER_LEFT_4_SEQUENCE=TGCAAGCAAACAACTTCTTG
PRIMER_RIGHT_4_SEQUENCE=ATGCACATCGATTCATAACCCT
PRIMER_LEFT_4=18,20
PRIMER_RIGHT_4=149,22
PRIMER_LEFT_4_TM=57.696
PRIMER_RIGHT_4_TM=60.591
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_03663 Ps_contig_mira-and-tgicl-ass_franssen_30649
SEQUENCE=ATGTTCAAAGGGACCCTATTTTTTTTCAACCTTTGCAAAGTGTCAAGCCACACTTGTTTTGTTTGTATTTTATGTGAAAACAACAATAANAAANGGAGGTGCTTGTAGTTGTTGTTTGTTGTTGGTGGGTGCGTAGGATAAGCTTCAAAATTGGGTTAACGTTAAGGGAGAATAGAAACAAGAAATGACAAGGTTTTTAAGATTATTGTGCTTTTATTAAGATAATAGTAATAACAACCCATTTAGTTGTATAGGTTACATTAAAAATATAAATGGTTTTTTTTGTGACCCTTTAGCTTCATTAATCTTTGTATTAATTTGGGTTGAATACCCATTTGAGAATTCTCATAAAGTTGCTTAAAGTTGCATGCTTTTGCAATTGGGGAAGCTTTCATGGAGGTCAATTTTGGATTTTGTTTGTTCTTGTTGGTTTTGTTGGCTCCTTTGACTTGTGCTGGCAGAGCCGCCATGAGCACCACCGCCGGTGGCGGTGGTGGTGGTGATGGTGGTGGTGATGGTTCAATTCAGAAGCTTGAAGTTCAGAAGCACTTGAAGAATTTGAACAGGCCACCGGTTAAGTCCATCAAGAGTCCTGATGGAGATATTATTGATTGTGTTCATGTTTCACATCAACCAGCTTTTGATCATCCTGACCTCAAAAGTCACAAGATTCAGATGAGGCCGAATTTCCATCCGGAAGGGAACAATTTTGGAGAAAGCAAAGTCTCTTCAAATTCAAAGCCTATAACTCAACTTTGGCACAAAAGTGGGAGGTGTTCCGAAGGAACAATTCCTATAAGAAGGACCAAAAAGGATGACATATTACGGGCTAGTTCTGTCCAAAATTTTGGAAAGAAGAAGCACAAAAGTACCCCTCAGCCGAAACCAGCAAAGCCGCTACCCGACCTCTTCACTCAGAGCGGTCACCAGCATGCCATAGCATATGTGGAAGGGGGAAATTTCTATGGAGCGAAGGCAACCATCAACGTTTGGGACCCGAAAATTCAACAACCTAATGAATTCAGCCTGTCTCAAATTTGGATTTTGGCCGGCTCTTTCAATCAAGATCTCAACAGCATTGAAGCAGGCTGGCAGGTCAGCCCTGATTTGTATGGTGACAATAACACTAGACTCTTCACTTATTGGACAAGTGATGCATATCAAGCAACTGGTTGTTATAATCTTCTTTGCTCTGGATTTATTCAAATTAACAATGATATAGCCCTTGGTGCAAGTATATCCCCACTTTCTAACTATGGTTCTTCCCAATATGATATAAGTCTCCTTGTGTGGAAGGACCCAAAAGAGGGAAACTGGTGGATGCAATTTGGGAATGACCATGTTCTTGGATACTGGCCAGCTCCTTTATTCTCTTACCTAACTGAGAGTGCTTCCATGATTGAATGGGGAGGTGAAGTAGTGAACTCTGAGTCTGATGGACAACACACCTCAACACAAATGGGAAGTGGACATTTCCCCGATGAAGGTTTCGGCAAAGCTAGTTATTTCAAGAACATTCAAGTTGTTGATGGCGACAACAAGTTAAGAGCTCCAAAAGATCTTGGAACTTACACTGAAAAAGAAAACTGCTATAATGTCAAAACCGGTAATGCCGGGGATTGGGGTACCTACTTCTATTATGGTGGTCCAGGTCGAAATCCTAATTGCCAGTGATCTTTTATGTTAAAATCATGGTTTTAAATTGCGGTCAGGTTTCTGCTACTATACAAATTTTTATACTCCGCAAAAATGCATGTGGTTTTAATAGAGTTGCGGAAACCTGATAATTTTTTATATTACTATTTTTAATTTTATATTTCGACCGCAATTTAAAACCATGTTTAACCTGGTGTTGTTTTGAATTGGTCAGTGTAGCTAGTAACTTGTGGTATCCTTAGATGATATTGGTGTAGAAGGTCAAAAATCTCATTTGAGGTCTCCAAAAATTGTTGGAGAAAAGGGGTGATGGGTTTGTTAGATTGACAAGAAAAATGTTTGTTCATTGTTTTCCATGTTTATATAAATATGTATATTGTCTGCTCCTTTCTCTGGAGCTGTGAATGTGAAAAGAATATTCTCTAATAAGAGCAATTTAAATTTTA
TARGET=490,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0568
PRIMER_LEFT_PENALTY=0.054514
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=GGGTTGAATACCCATTTGAGAA
PRIMER_RIGHT_SEQUENCE=GGACTCTTGATGGACTTAACCG
PRIMER_LEFT=320,22
PRIMER_RIGHT=592,22
PRIMER_LEFT_TM=60.055
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=273
PRIMER_PAIR_PENALTY_1=0.0838
PRIMER_LEFT_1_PENALTY=0.054514
PRIMER_RIGHT_1_PENALTY=0.029253
PRIMER_LEFT_1_SEQUENCE=GGGTTGAATACCCATTTGAGAA
PRIMER_RIGHT_1_SEQUENCE=CTTCTGAATTGAACCATCACCA
PRIMER_LEFT_1=320,22
PRIMER_RIGHT_1=530,22
PRIMER_LEFT_1_TM=60.055
PRIMER_RIGHT_1_TM=59.971
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.3280
PRIMER_LEFT_2_PENALTY=0.325733
PRIMER_RIGHT_2_PENALTY=0.002310
PRIMER_LEFT_2_SEQUENCE=GTTTTGTTGGCTCCTTTGACTT
PRIMER_RIGHT_2_SEQUENCE=GGACTCTTGATGGACTTAACCG
PRIMER_LEFT_2=429,22
PRIMER_RIGHT_2=592,22
PRIMER_LEFT_2_TM=59.674
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.3550
PRIMER_LEFT_3_PENALTY=0.325733
PRIMER_RIGHT_3_PENALTY=0.029253
PRIMER_LEFT_3_SEQUENCE=GTTTTGTTGGCTCCTTTGACTT
PRIMER_RIGHT_3_SEQUENCE=CTTCTGAATTGAACCATCACCA
PRIMER_LEFT_3=429,22
PRIMER_RIGHT_3=530,22
PRIMER_LEFT_3_TM=59.674
PRIMER_RIGHT_3_TM=59.971
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=102
PRIMER_PAIR_PENALTY_4=0.4267
PRIMER_LEFT_4_PENALTY=0.424392
PRIMER_RIGHT_4_PENALTY=0.002310
PRIMER_LEFT_4_SEQUENCE=GTTCTTGTTGGTTTTGTTGGCT
PRIMER_RIGHT_4_SEQUENCE=GGACTCTTGATGGACTTAACCG
PRIMER_LEFT_4=419,22
PRIMER_RIGHT_4=592,22
PRIMER_LEFT_4_TM=60.424
PRIMER_RIGHT_4_TM=59.998
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=174
=
PRIMER_SEQUENCE_ID=SSR_03664 Ps_contig_mira-and-tgicl-ass_franssen_30657
SEQUENCE=AATTCCAAATATATTTAATAATTGTCGTTTATATTCATGGATTCTCTTCACCTTCCGTAGTTCAGCCAGTTTCTCAGTATTTAGGACCCAATCTTCTGTGCCTATCCACTGAGTTATTATTTTACTCAAATCTGGATCGCAGAACCTAATCCATCTCCTCGGTGTCACACCATTTGTTTTGTTTTGGAATTTCTCAGGCCACAACTTATAAAATGCATTGAACACATCATCCTTGACAATTTCACTATGTATCTCTGCAACCCCATTCACTGCATGACCACCCACAACACAAAGATTGGCCATACGAACCAATTTTGGCGGTACTGGTACTGGCTCAGGCAACTCCTTTTTCTTATCCTTAATGGAGCTTTTATTCGTCCCATCCTTTTCAGCAATTGCTTTTTCCACTTCATCTTCATTACCATCATTGTCTTTTTCTCCTCCTCCTTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTCCTCCTTCTTCTTTAGAAATTTGCACTTCCTCACTAGAAATATCAGGCCGGCCTTCCTTTGGTTTTAACTTAGCATATCTGCAAATTCTGCAGGCAATACAACATTTTCTAGTATTCTCATTTCCTTCAATTTTTTATCAAGTACGTCGGAATCTGCTGTGCCATATTCTGCGATTATGGTCCGAATCAGCTCCTCATCAATCATCTCTATAATCTCGACATGGCGTGGAAGCAGTTTCTCCATAAGGTCCATGCTCCATTTCTCTAATGCCTCCGGAAGAACAGTATGGTTTGTGTACGCTACAGTCCGTTGGGTGATATTCCAAGCATCCTTCCAGCTTAAACCCTTTATATCTATCAGGATTCTCATCAGCTCTGGGATGCACAAAGTTGGGTGAGTATCATTCATCTGCACTGCAACCTTTTCAGGAAAATCTTCCCAATTCACACTTGTCCCCGATCTTCTCTCAAAACGAGCAATGATATCTTGAAGAGAAGCAGAACATAAAGTATATTGTTGCTTGAGGCGAAGGGTTTTTCCCTCTATAGATTCATCCCCAGGGTAGAGTATATAGCAAATCTTTTCAGCATTTGCTAGAGCCTCAGATGCTTCAGTGTGCCTTCCAGAATTAAAAGCATTTAAATCAAATTCTTCGGATGCAGCTTTTGTAGACCAAAGTCTAAGGTTAATTGTGCTTCTGGTCTTATATCCAGGTATGGGGACATCGTGTGCAACAGCTTTGATATCTTCTCCTCCAACCCAATGTTTTTTACCATCTGAACCTGAAACAACTTTGCCATAGAACTTTACAGGGTATGAGATGTCATTTCTAATGATCTCCCAAGGATTGCCCATCTCAAGCCAATCTTCAGCAACTTCCTCTTGCCCATCTTTGGTGATTCGTTGTTTGAATAAGCCATACTTGTATCTCAGTCCATAACCCCATGCTGGATAATTCAAGGTTGCCAAAGAGTCCAAGAAACATGAAGCAAGTCGTCCAAGACCCCCATTTCCAAGTGCAGCATCCGGCTCCTGGTGTGCCACATCCTCTAATTTATAACTAAGCTGGCTTAAAGCCTCTGCATAGGGACCAGTTAGCTCTAAATTGCCAATTGCATTCAATAATGCTCTACCCTGTAAAAATTCCATTGAAAGGTAATATGCCTGCTTAACATTCAGCTTCTCATAGTAATCATAAGTAGCATTCCAGTTTATTATGAGAGCATCACGAACACTCTGTGCAGTTGCAATGAAAGTCTTTGGTAGGAAGTCCTTACGTAATTTTCCGTACCGGGAAGAAAAAC
TARGET=451,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3242
PRIMER_LEFT_PENALTY=0.215225
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=ATGTATCTCTGCAACCCCATTC
PRIMER_RIGHT_SEQUENCE=CCGGCCTGATATTTCTAGTGAG
PRIMER_LEFT=246,22
PRIMER_RIGHT=528,22
PRIMER_LEFT_TM=60.215
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.3242
PRIMER_LEFT_1_PENALTY=0.215225
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=ATGTATCTCTGCAACCCCATTC
PRIMER_RIGHT_1_SEQUENCE=CGGCCTGATATTTCTAGTGAGG
PRIMER_LEFT_1=246,22
PRIMER_RIGHT_1=527,22
PRIMER_LEFT_1_TM=60.215
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=0.4136
PRIMER_LEFT_2_PENALTY=0.304643
PRIMER_RIGHT_2_PENALTY=0.108929
PRIMER_LEFT_2_SEQUENCE=TTATTCGTCCCATCCTTTTCAG
PRIMER_RIGHT_2_SEQUENCE=CCGGCCTGATATTTCTAGTGAG
PRIMER_LEFT_2=370,22
PRIMER_RIGHT_2=528,22
PRIMER_LEFT_2_TM=60.305
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.4136
PRIMER_LEFT_3_PENALTY=0.304643
PRIMER_RIGHT_3_PENALTY=0.108929
PRIMER_LEFT_3_SEQUENCE=TTATTCGTCCCATCCTTTTCAG
PRIMER_RIGHT_3_SEQUENCE=CGGCCTGATATTTCTAGTGAGG
PRIMER_LEFT_3=370,22
PRIMER_RIGHT_3=527,22
PRIMER_LEFT_3_TM=60.305
PRIMER_RIGHT_3_TM=60.109
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=0.4304
PRIMER_LEFT_4_PENALTY=0.321492
PRIMER_RIGHT_4_PENALTY=0.108929
PRIMER_LEFT_4_SEQUENCE=AGCTTTTATTCGTCCCATCCTT
PRIMER_RIGHT_4_SEQUENCE=CCGGCCTGATATTTCTAGTGAG
PRIMER_LEFT_4=365,22
PRIMER_RIGHT_4=528,22
PRIMER_LEFT_4_TM=60.321
PRIMER_RIGHT_4_TM=60.109
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=164
=
PRIMER_SEQUENCE_ID=SSR_03665 Ps_contig_mira-and-tgicl-ass_franssen_30658
SEQUENCE=ATTCAGAACAGAAAAGAAAAAAATTCACATTTGACTCTGCAGCTAGAGTGAGACAACTGACAAAAGCGATGCCGGAGGAAACGACGTCGATTGATTACGCCATGGAGGCGGCTTCCGGGCCCCACTTTTCCGGCCTTCGACTCGACGGCGGCCGACTGTCATCTTCTTCTCCAACCTCTTCTCTCGCTCCCAATTCAACCCTAACCGCAGATTCTCTTCTCAATCAACCCTTCGTTATTGGTGTTTCCGGTGGAACTGCTTCTGGAAAGACCACCGTTTGTGATATGATCATTCAACAGCTTCACGATCATCGCGTAGTTCTTGTTAATCAGGATTCGTTTTATCGTGGGTTGAAACCGGACGAGTTGGAACGCGTTCATGAGTACAATTTTGATCACCCTGATGCTTTCGACACAGAGCAACTGTTAGAGTGTATGAAGCAGCTCATCAGTGGCCAAAGAGTTAAAATTCCCATTTACGACTTCAAGAAGCACCAACGCTCTTCTGATAGTTTTCGTCAGGTTAATGCATCCGATGTCATTATATTGGAGGGAATTCTTGTATTCCATGATCAAGGTGTCCGTGATCTGATGAATATGAAGATCTTTGTTGACACAGATGCTGATGTGAGGCTTGCTCGTAGAATTAGACGTGACACAGTGGAAAGGGGCAGAGACATAAACTCCGTTCTTGAAATGTATGCAAAGTTTGTTAAGCCTGCTTTTGATGACTTCGTTCTACCATCAAAGAAATATGCCGATGTGATCATTCCTCGTGGAGGTGATAATCATGTTGCCATTGATTTGATTGTGCAACACCTCCGTACAAAGCTTGGTCAACATGATCTATGCAAAATATATCCAAACGTGTATGTTATTCAATCTACATTCCAGATAAGGGGGATGCATACATTGATTCGTGACACAGAAATATCAAAGCATGATTTTGTATTTTATTCTGATCGACTTATACGCCTGGTTGTTGAGCATGGTCTAGGTCATCTTCCTTTTACCGAAAAGCAAGTTGTTACTCCAACAGGATCTGTCTATACTGGTGTTGATTTCTGCAAGAAATTATGTGGTGTTTCAATTATTCGAAGTGGTGAAAGCATGGAAAATGCACTTCGTGCATGTTGTAAAGGCATTAAAATTGGAAAAATCCTGATTCACCGGGATGGAGACAATGGAAAACAGCTTATCTATGAGAAGCTTCCCAAGGATATCTCAGAACGGCATGTCTTGCTTCTTGACCCTGTCCTTGCTACAGGTAACTCAGCCAACCAAGCAATTGAATTACTCATTCAAAAAGGAGTGCCAGAATCCCACATTATATTTCTGAATCTCATTTCTGCTCCTGAGGGAATCCACTGTGTGTGTAAAAGGTTCCCATCGTTGAAAATTGTCACATCTGAGATTGATGTTGCATTAAATGAAGAGTTCCGTGTTATCCCAGGATTGGGTGAATTTGGTGATCGCTACTTTGGCACCGATGATTGATCCTGCAGCGTGTCGAAGGTGGATACATCTTTTAGTCAACAAATGTCTTGGCTGCATTGTATTGAAATATTGAAGATTTAGCAAATGGCTGCTATGTGATTAGAATAATATGAATGCAAACTCATTCTTTGGCTGATATGCATGATTGGTTTTCAGCTTATTGAATGTAGAGATAAATTCTAGCTAGATGATGATGATGCTATCATACAGAAGTTCTGAGTAGACAACTCTATCTAGTTCAGCCAAGGTTATTTAAGGAAAATTGTTGGAGTGGGCCGACCAAACTACAAACTA
TARGET=1683,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2343
PRIMER_LEFT_PENALTY=0.024665
PRIMER_RIGHT_PENALTY=0.209637
PRIMER_LEFT_SEQUENCE=ATGTCTTGGCTGCATTGTATTG
PRIMER_RIGHT_SEQUENCE=CCCACTCCAACAATTTTCCTTA
PRIMER_LEFT=1538,22
PRIMER_RIGHT=1770,22
PRIMER_LEFT_TM=60.025
PRIMER_RIGHT_TM=60.210
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.3195
PRIMER_LEFT_1_PENALTY=0.109882
PRIMER_RIGHT_1_PENALTY=0.209637
PRIMER_LEFT_1_SEQUENCE=GAAGATTTAGCAAATGGCTGCT
PRIMER_RIGHT_1_SEQUENCE=CCCACTCCAACAATTTTCCTTA
PRIMER_LEFT_1=1567,22
PRIMER_RIGHT_1=1770,22
PRIMER_LEFT_1_TM=59.890
PRIMER_RIGHT_1_TM=60.210
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.3327
PRIMER_LEFT_2_PENALTY=0.123068
PRIMER_RIGHT_2_PENALTY=0.209637
PRIMER_LEFT_2_SEQUENCE=GCAAATGGCTGCTATGTGATTA
PRIMER_RIGHT_2_SEQUENCE=CCCACTCCAACAATTTTCCTTA
PRIMER_LEFT_2=1576,22
PRIMER_RIGHT_2=1770,22
PRIMER_LEFT_2_TM=60.123
PRIMER_RIGHT_2_TM=60.210
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.3478
PRIMER_LEFT_3_PENALTY=0.138114
PRIMER_RIGHT_3_PENALTY=0.209637
PRIMER_LEFT_3_SEQUENCE=GTCTTGGCTGCATTGTATTGAA
PRIMER_RIGHT_3_SEQUENCE=CCCACTCCAACAATTTTCCTTA
PRIMER_LEFT_3=1540,22
PRIMER_RIGHT_3=1770,22
PRIMER_LEFT_3_TM=60.138
PRIMER_RIGHT_3_TM=60.210
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=231
PRIMER_PAIR_PENALTY_4=0.8306
PRIMER_LEFT_4_PENALTY=0.620981
PRIMER_RIGHT_4_PENALTY=0.209637
PRIMER_LEFT_4_SEQUENCE=GCAAACTCATTCTTTGGCTGAT
PRIMER_RIGHT_4_SEQUENCE=CCCACTCCAACAATTTTCCTTA
PRIMER_LEFT_4=1611,22
PRIMER_RIGHT_4=1770,22
PRIMER_LEFT_4_TM=60.621
PRIMER_RIGHT_4_TM=60.210
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_03666 Ps_contig_mira-and-tgicl-ass_franssen_30661
SEQUENCE=GNAGAATAAAATTGGCCATTTATTAAAAACAATTGCAAGGGAGAAGGGTGGGAAATATCATCAATTAATTGTTGAAATAGAGCAACAAACAACACAAATTACAATACAAATTCAATATTATACAACAATGATAGTAGCATGGTAACATGAGTTATTCCCCTATAGACATTAAACAGAATCAATATTCTTCAGGCACAGGAAGATCCTCCTTATCTGGTACATCAACCCATTCTCCATCTTCCCTTCTCACCAAACCTTTATTCTTCTCCACCCACCATGAACCTGGCACATGGCACTCTTCCTTCAAGTAATCACAAGACTTATTCACCAAAGCCACACTCCTCTTCACCTTCAACTCAAACTCACCCTCACTCCCATTCCAACCAGCCACCACATGCAACATAGCCTGCAAATTATGCCAATCACTCGGATTCTTCGAATCACTCAAACTCGTTGATTTCCTTGTATCAATCACCAACTCTACACCTGTATACTCATACCCCAATAACTTCCCTGGATAATGCGGTATAAGATCAATCACATTCCTCACGTGTAACACCTTCAAATTCGGAAACTTTTTGAACTTGTCATTAAATGCTTTGTTCCCAACTTGTGGGGAACCAAACACAAAGGCTGCAACGGGAATGTCTGTTACACCATTCTCGACCAAATCAAAAGCACTTACAACTGATAGACTTGCTCCTAGACTGTGTCCCACTAATACCACACTTGGATTTTCATTCTTGTAGATGTTCAGCAAACTTTTTATCTTGTTTAGAATCTGTGTTCTCACGCTTGTTTTTGTGAAAGGAGATTTTGGGTCGTCTGAGGTGTATATTGTCAGCCAACCCTTCATTACTTTCGGCAGCCTTTTGTCATCTTCATTGTCACTGCTGCTGCTACCATCATCTTTATTCTTGTTTGGGACGTTGAGTTGGCTGAGACTTTTAGCACTCAACAAGCCAGATGCAGACTCAGGCGCAGCACCAAACACGTTAATCCATTCAAGATCCCTCGTGGTACCACGCCACACAATATATATTTCACGGCGACCCAATTCTCTACTCCGTTGATCAGAAGTAACAGCTATGTAACCGATCCAGTTAGACTCACGGTCCCAACTCTCACGTGAAAGCGAGTGAAGCAAGAAGGCTTCAGGAACAGAGACACGTGCAGTAGCGTAAAGGAAAGAAACAACAGTGTAGTGTGTTGGGTTTTCAAGCATGACTTTGTTAAAGAACGTGGATTTTCCGTAACGGCTTGAACCGCAGTAGATAGAGTGTTGGTCGTTGTTGAAAGCATCGTAGGTTGTTTGTATGAAGTCACCGCAACGGAGTATGAGGTTGCGGAGGTTGAGGTCTAGTGGGTTTAATAGTGTTTCCCAGTTGTTGCTTCCTAGGAGTTGGTTCCATGTGTCTTGGTTTGTTGTTGTTGTTGATGTTGTTGATGATGATGTTGGTGGTGGTGGTGTTTCTGTGTCTGTTGCCATGTTTATGGGACTTTTTTTGTGTGATAATCCCATGTTATAGGGAGCGTGTGTCTCYGTGTTTTTGAGGGAGTGGATATATATTGTGAGGAGTTGATGAGGGAAGAGAAGTATGTGGTNAGATGAGAAAGAA
TARGET=1423,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03667 Ps_contig_mira-and-tgicl-ass_franssen_30661
SEQUENCE=GNAGAATAAAATTGGCCATTTATTAAAAACAATTGCAAGGGAGAAGGGTGGGAAATATCATCAATTAATTGTTGAAATAGAGCAACAAACAACACAAATTACAATACAAATTCAATATTATACAACAATGATAGTAGCATGGTAACATGAGTTATTCCCCTATAGACATTAAACAGAATCAATATTCTTCAGGCACAGGAAGATCCTCCTTATCTGGTACATCAACCCATTCTCCATCTTCCCTTCTCACCAAACCTTTATTCTTCTCCACCCACCATGAACCTGGCACATGGCACTCTTCCTTCAAGTAATCACAAGACTTATTCACCAAAGCCACACTCCTCTTCACCTTCAACTCAAACTCACCCTCACTCCCATTCCAACCAGCCACCACATGCAACATAGCCTGCAAATTATGCCAATCACTCGGATTCTTCGAATCACTCAAACTCGTTGATTTCCTTGTATCAATCACCAACTCTACACCTGTATACTCATACCCCAATAACTTCCCTGGATAATGCGGTATAAGATCAATCACATTCCTCACGTGTAACACCTTCAAATTCGGAAACTTTTTGAACTTGTCATTAAATGCTTTGTTCCCAACTTGTGGGGAACCAAACACAAAGGCTGCAACGGGAATGTCTGTTACACCATTCTCGACCAAATCAAAAGCACTTACAACTGATAGACTTGCTCCTAGACTGTGTCCCACTAATACCACACTTGGATTTTCATTCTTGTAGATGTTCAGCAAACTTTTTATCTTGTTTAGAATCTGTGTTCTCACGCTTGTTTTTGTGAAAGGAGATTTTGGGTCGTCTGAGGTGTATATTGTCAGCCAACCCTTCATTACTTTCGGCAGCCTTTTGTCATCTTCATTGTCACTGCTGCTGCTACCATCATCTTTATTCTTGTTTGGGACGTTGAGTTGGCTGAGACTTTTAGCACTCAACAAGCCAGATGCAGACTCAGGCGCAGCACCAAACACGTTAATCCATTCAAGATCCCTCGTGGTACCACGCCACACAATATATATTTCACGGCGACCCAATTCTCTACTCCGTTGATCAGAAGTAACAGCTATGTAACCGATCCAGTTAGACTCACGGTCCCAACTCTCACGTGAAAGCGAGTGAAGCAAGAAGGCTTCAGGAACAGAGACACGTGCAGTAGCGTAAAGGAAAGAAACAACAGTGTAGTGTGTTGGGTTTTCAAGCATGACTTTGTTAAAGAACGTGGATTTTCCGTAACGGCTTGAACCGCAGTAGATAGAGTGTTGGTCGTTGTTGAAAGCATCGTAGGTTGTTTGTATGAAGTCACCGCAACGGAGTATGAGGTTGCGGAGGTTGAGGTCTAGTGGGTTTAATAGTGTTTCCCAGTTGTTGCTTCCTAGGAGTTGGTTCCATGTGTCTTGGTTTGTTGTTGTTGTTGATGTTGTTGATGATGATGTTGGTGGTGGTGGTGTTTCTGTGTCTGTTGCCATGTTTATGGGACTTTTTTTGTGTGATAATCCCATGTTATAGGGAGCGTGTGTCTCYGTGTTTTTGAGGGAGTGGATATATATTGTGAGGAGTTGATGAGGGAAGAGAAGTATGTGGTNAGATGAGAAAGAA
TARGET=1457,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03668 Ps_contig_mira-and-tgicl-ass_franssen_30662
SEQUENCE=TACGTTNTCGGGGACAATCACTTTCTTCTCTCTTCGCATCACAGCCACTAATTTTCCCCCTTCTCACTCTCTTTCCAAACCCTAACCCTAATTCTGATTCCATCACCACCATGGCCAAAAAACGAAAGCTTCGATCTTCCGACCCCGAACCCACCAAGCCACTCGAAACCGAACCAAAGCCAGAAGCAGCCGAAGCACAACCCGAACCAACTACAGACCAAGAACCAAGCCATGAAAACATGGCCGTCGATGAAGAAATTGCACCGGTAGAAGAAGATCAACCTCAAAACGAAGAACAAGTAGGTGAAGGCGAAGCAGAAGGAGAAGAACAAGAACAAGTAGAAGAAGATGAAGACGAAGAGCAACAAGGAGAAGACGAAACCCTAGAAAACCGAAACCAACAACAAGACGCCGCGGAAGTTTCAAATCCAACCGCCGTTGAGCAGGCTGAGACCGTCGAAGCTAACGGTGCTGAGGAAACTAACAACGATGAAGAAGAGGAAGATCTTGATTTGGAAGACGAACCTGTTGAGAAGCTTCTAGAGCCTTTCACGAAGGAGCAATTACACGCTCTCGTGAAGCAAGCTATTGAGAAGTATCCAGATCTGTCGGAGAATGTTCGTCAGCTGGCTGATGTGGACCCTGCTCACAGGAAAATTTTCGTCCATGGACTTGGATGGGATGCAACCGCCGAAACCCTAACCAGTGTGTTTCAGAAATACGGTGAAATTGAAGACTGTAAGGCGGTTACTGATAAGTTATCTGGAAAATCAAAGGGGTATGCTTTTATTTTGTTTAAGCATAGGAGTGGTTGTCGCAGGGCGTTGAAGCAGCCTCAGAAGCAGATTGGTAACAGAACAACATCTTGTCAGTTGGCCTCTGCTGGACCTGTTCCGGCACCTCCACCGAATGGTCCTCCGGTATCAGAGTATACCCAGAGGAAGATTTTTGTAAGCAATGTGAGTTCTGATATTGATCCTCAGAAATTGCTTGAGTTTTTCAAACAGTTTGGGGAAGTTGAAGACGGTCCTTTGGGTCTTGATAAGAATACTGGAAGGCCAAAGGGATTTGCTCTGTTTGTGTACAAGTCTGTTGAGAGTTCTAAGAAGGCCTTGGAAGAGCCAAATAAGATTTTTGAGGGTCACACTTTGTATTGTCAGAAGGCTGTTGATGGTCCTAAGGGATACAAGGGTCATTATCAGCAGCCTCAACATCATCATCATCAACAGCATCATTCCCACCATCACCATCACCAGCCTCACTACAATTCAAGGAAAGAGAAGAACAAGTATTCCTCTGGTGGTGGGCCATCACACGGTGGTGGCGGTGGTCACTTGATGGCACCTCCTGGGCCTGTTGCGGGTTACAATCCTGGAGTCCCGGCTCAAGGATTGAATCCAGCACTTGGTCAGGCTTTATCAGCTTTGCTTGCTAATCAAGGAGCTGGATTGGGGCTTGGAAATTTGCTAGGAGGTCTTGGTGGTGCTCCTGTGAACCAAGCTGGGCCACCTCCTGCTGCGTATGCTAACCAGGCTGCTATGGGATATGGAAACCAGCCGGGGTTGCAACCACAGTACCAGAATCCTCAGTTGGGGCAGAGTGGTGGTGCTAGACCTCATCCTGGTCCAGGTGGTGCTCCTTACATGGGTCACTAGATACCCTCATAGATGCATCAAACATTTAGTTTTGGACTCTGCTGATTCTTCTGAAGTACCAGGCCCTATTCAAATGCATCTTTTAAACTTGTAACTTTTATTAATTACTAAAGAACCAGATTCTGAAACCTGTATTTTCTTTCTATAGTAGAAGATTTTTGCCAAGACTTTTTTGAAAATCATGCAGTACCTCTTGTCACTTTATGGAATTTGAGGCATGTGTTGAATTTTGGGGCAGAGAAAACTTTGTTACAATAACTTCTATGCGTGCTATAGCCGTTGCTATTTTTTTGGATTTCTAATTATTGTTTTNATCATTTAGTTGTTTATCTTCCGTAAAAGCATGACTAGGGGAAAAGTGTAAGTTCTGTAATTTTACTTTGTCTT
TARGET=1212,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1234
PRIMER_LEFT_PENALTY=0.075241
PRIMER_RIGHT_PENALTY=0.048131
PRIMER_LEFT_SEQUENCE=GGTCCTAAGGGATACAAGGGTC
PRIMER_RIGHT_SEQUENCE=AGCAAAGCTGATAAAGCCTGAC
PRIMER_LEFT=1172,22
PRIMER_RIGHT=1428,22
PRIMER_LEFT_TM=60.075
PRIMER_RIGHT_TM=60.048
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.1623
PRIMER_LEFT_1_PENALTY=0.075241
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=GGTCCTAAGGGATACAAGGGTC
PRIMER_RIGHT_1_SEQUENCE=AAGCTGATAAAGCCTGACCAAG
PRIMER_LEFT_1=1172,22
PRIMER_RIGHT_1=1424,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.1785
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.048131
PRIMER_LEFT_2_SEQUENCE=AGAAGGCTGTTGATGGTCCTAA
PRIMER_RIGHT_2_SEQUENCE=AGCAAAGCTGATAAAGCCTGAC
PRIMER_LEFT_2=1158,22
PRIMER_RIGHT_2=1428,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=60.048
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=0.1785
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.048131
PRIMER_LEFT_3_SEQUENCE=GAAGGCTGTTGATGGTCCTAAG
PRIMER_RIGHT_3_SEQUENCE=AGCAAAGCTGATAAAGCCTGAC
PRIMER_LEFT_3=1159,22
PRIMER_RIGHT_3=1428,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=60.048
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.1803
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=0.168114
PRIMER_LEFT_4_SEQUENCE=AACAGTTTGGGGAAGTTGAAGA
PRIMER_RIGHT_4_SEQUENCE=TTGAATTGTAGTGAGGCTGGTG
PRIMER_LEFT_4=1002,22
PRIMER_RIGHT_4=1271,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=60.168
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_03669 Ps_contig_mira-and-tgicl-ass_franssen_30664
SEQUENCE=AGATTCGAATGGATAATAATAACAACRCRRNANNNGNGRTATCAANRCATRTNYACCATTCTCATTCCCATCAAAGCCATGCCAATGGAGGTTTTCTCTCTCACTTCAACTACCATTCCCTCCACTCTCACACGTAGAGACACTGCAGCAGATAAACCTTCCCCTCATCTCAACCTTTCCAAATACTCACATTTCATGAGGTACCCTCTTACCACAACACTCACCAACAACAGAATCCGCAGTAGCAGTAGCAGTAGCAGTAGCATAAGAGCTCAAGCTTCAGGTTCAACAAAATCTAGTACTGCTGAGGGGATTCCTGAAAAAACAGATTCCAAGGATGATAATCTAGTTTTTGTTGCTGGTGCTACTGGAAAAGTTGGTTCAAGAACAGTCAGGGAGCTTATAAAGCTTGGTTTTAAAGTTAGAGCTGGAGTAAGGAATGCTCAGAAAGCTGGTGCATTGGTTCAGAGTGTTAAACAATTGAAGCTTGATGGTGCAAGTGGAGGGGGTGAAGCTGTAGAGAAGCTTGAAATTGTGGAATGTGATTTGGAGAAGGCAGATCAAATTGGATCAGCATTAGGGAATGCATCAACTGTGATATGTGCTATTGGTGCTAGTGAGAAGGAAATTTTCGACATTACTGGACCTTGTCGAATCGATTACCGAGCTACCAAAAACCTTGTTGATGCTGCAACTGTTGCCAAAGTAAATCACTTCATATTGGTTACTTCATTGGGGACAAACAAATTTGGTCTTCCTGCAGCTATTCTCAATTTATTTTGGGGAGTCCTGATTTGGAAAAGGAAGGCCGAAGAAGCATTGCTAGCAAGTGGAATTCCATATACAATAGTGAGACCTGGTGGCATGGAGAGGCCGACTGACGCTTACAAGGAAACACATAATGTAACTCTGTCAACTGAAGATACTTTATTTGGGGGTCAAGTTTCAAACCTTCAGGTTGCTGAACTCATGGCAATCATGGCCAAGAATCCCGATCTTTCATACTGTAAAATAGTGGAGGTTATTGCCGAGACAACTGCGCCACTAACACCTGCCGAAAAACTTCTTACAAGGATACCTTCTCAACGCCCTTACATTCCTTCGCCAAAGAAGGTACAGAAAGCAGATACAGCAACTGTGAGTAATACAGGTCCCTCTGCTAATGTTGTAGCAGAAGTATCTAGTATTGCACCTCAAAAAGAAACAGCATCAAAACCAGTGGCGAAAACGGAACAGCCACTTTCTCCTTATACCGCTTATGATGATTTAAAGCCACCATCATCTCCTTCTCCAACCAAGCCTAGTGAGAAGAAACAGATAAACATCAGTGATGCAGTTCCAACACCTATTTCTTCAGATACTCCAAGTAGCATTCAAGAAATAGATGGCATTTCTCAGACAACATCTTCCTCAAAAGGGAAGGAGTCTCTATCTCCTTACGCAGCATATCCTGATTTAAAGCCTCCTAGTTCACCATCTCCGAGTGTACCAACTACATCACTATCGAAGAGAGATACAGTAGTAGTATCAAGCAATGGACCAGCTCAACTTTCTGTGGAAGATACACCAAAGAATGAAGAACAGCACCTTCATGAACCAAAGTCAAGGCCACTTTCACCTTATGCAATGTATGAGGACTTGAAGCCTCCTGCATCACCATCACCTTCCTTCAGAAAATCCTAGGAACTGCCTCCAGCCTATGGAATCAGACAAGGATTATTTTATTGAAGAGTTTATGCAGCAACAATTAAGGAGGATTGTCACTGAAAGTATGAAGATCCGAAGCATTTATTTGAATGGAAATTTTCATGCGATTTATTTCTTGATAACCTTTTGAGGATAGTCACTCAAAAAGCACTGTTGTTTTTATATATATAACTTACCTTTCCTATAATGCAAGTTAAATAAGGAAAAGGTATTACTTTTCAATATCATTTTTCCACTTTATATTATTTTCCTTGTCATGTTTCCCACTCTCAAAG
TARGET=1868,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03670 Ps_contig_mira-and-tgicl-ass_franssen_30670
SEQUENCE=CCAAAACTAGCCTCCTTTACCAAGACAGCAAAATACTTATTACCTTACGCTCAAGCACAATCATAAGCACTTATAGCCAATATATTGGTCATCATAATCATTGTCGGGACTCTACCGAGTTCAGGGTAAGAGAAAACACATACACCACCGATATTCAACAATCACTCCTCAAACAGATAATCATAGAAAAACAACAATATAAGTTATAACCATAATCCATAACCTGTAATCCACTAGTATCTCCGACTAGGGTAGCGATCACCAGGGTAGCGATCACTAGGGTAGCGATCCATAAAGCGACCTGAAGGACACTCATATGCCAAGTGACCACGGCCTCCACAATTGTGACAAATCATCAAGGGGCCCATGCAGTCACGGCTCATATGACCCAACTGTTGACAGTTCCTGCACACGATATCACGGTAACCACCACCACCACCCGCTCCACCAGCACCACGGAAGCTNGCCTCTCGCCTACTTGTAGTAACTTCCTAACCTAAAAGTTTTAAACGTTTTGGGTTTTTACGGTTAACGTTTTTTACGGGAACCTTAGTTTTCGGTTACCG
TARGET=426,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5804
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=0.448106
PRIMER_LEFT_SEQUENCE=TGAAGGACACTCATATGCCAAG
PRIMER_RIGHT_SEQUENCE=CGAAAACTAAGGTTCCCGTAAA
PRIMER_LEFT=302,22
PRIMER_RIGHT=558,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=59.552
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.5804
PRIMER_LEFT_1_PENALTY=0.132262
PRIMER_RIGHT_1_PENALTY=0.448106
PRIMER_LEFT_1_SEQUENCE=CTGAAGGACACTCATATGCCAA
PRIMER_RIGHT_1_SEQUENCE=CGAAAACTAAGGTTCCCGTAAA
PRIMER_LEFT_1=301,22
PRIMER_RIGHT_1=558,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=59.552
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.7135
PRIMER_LEFT_2_PENALTY=0.265389
PRIMER_RIGHT_2_PENALTY=0.448106
PRIMER_LEFT_2_SEQUENCE=ACTGTTGACAGTTCCTGCACAC
PRIMER_RIGHT_2_SEQUENCE=CGAAAACTAAGGTTCCCGTAAA
PRIMER_LEFT_2=391,22
PRIMER_RIGHT_2=558,22
PRIMER_LEFT_2_TM=60.265
PRIMER_RIGHT_2_TM=59.552
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.7652
PRIMER_LEFT_3_PENALTY=0.132262
PRIMER_RIGHT_3_PENALTY=0.632953
PRIMER_LEFT_3_SEQUENCE=TGAAGGACACTCATATGCCAAG
PRIMER_RIGHT_3_SEQUENCE=TACTACAAGTAGGCGAGAGGCN
PRIMER_LEFT_3=302,22
PRIMER_RIGHT_3=485,22
PRIMER_LEFT_3_TM=60.132
PRIMER_RIGHT_3_TM=59.367
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=10.3500
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.7652
PRIMER_LEFT_4_PENALTY=0.132262
PRIMER_RIGHT_4_PENALTY=0.632953
PRIMER_LEFT_4_SEQUENCE=CTGAAGGACACTCATATGCCAA
PRIMER_RIGHT_4_SEQUENCE=TACTACAAGTAGGCGAGAGGCN
PRIMER_LEFT_4=301,22
PRIMER_RIGHT_4=485,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=59.367
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=10.3500
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_03671 Ps_contig_mira-and-tgicl-ass_franssen_30671
SEQUENCE=CAACTTCAACACAGTAGCTTATTAAGGTGGCGGGATGATGCATAATAAAAACCATGCTTCTTACATGCAGCAAAATCTTATTCCTAGCTCAAGCACAATCATAAGCACTTATAGCCTATATATTGGCATCGTGATCCATTGTCGTGACTCTACCGAGTTCAGAGTAAGAGAAAACACATACAACACCGATATTCAACAATCAGTCCTCAAACAGATAATCATAGAAAAACAATATAAGTTATGACCATAATCCATAACCTGTAATCCACTAGTATCTCCTACTAGGGTAGCGATCACCAGGGTACCGATCACTAGGGTAGCGATCCATAAAGCGACCTGAAGGACACTCATATGCCAAGTGACCACGGCCTCCACAATTGTGACAAATCATCAAGGGGCCCATGCAGTCACGGCTCATATGACCCAACTGTTGACAGTTCCTGCATACGACATCACGGTAACCACCACCACCCGCTCCACCAGCACCACGGAAGCTGCCTCTGCCACTGTGATCTCCAATAATGTTTGATTTTGGACATTGTCTAGCCACATGTCCAGAAACATTGCAGACATTGCAAATTGGGTCATTTGGACAATCACGTGCCAAGTGCCCTGTTTTCCTACAGTTATTGCAAGCCTTCTCATTCGTGCATTCAACAGCAATGTGGCCCTGCTTATAACAGTTATGGCACAACCTTAAGTCCCCTGGGGGCATTGCTGGAGCTGAGCACTCCCTGGCACGGTGGCCAGTTTTACCACAGGTGTGGCAAATGCCCTCATTTGGACAACTGCTGGCCATGTGGCCAGGTTCTTTGCAATTCCAGCACACTGATTTTGTGGAGCATTCAGAAGCAATGTGCCCAGGAAGGCCACAATTGTGGCAAACTGCAACATTAGGGCACTCTCTAGCATAGTGACCAGGTCTCTTGCAGTTCTTGCACAAATTGTCTCGGCTGAAACCCCGACTTGAATCTCTCCTGTAAGGAGCATCACGATAGGAATGTCGATCAGAGCGAATCTTACGGATCCCTGGGCTACGACTCCTACTCCTGCTTCGGCTTCGGCTGCGGCTGTCTGAACTCATTTTCACTTTCTTTCTTTCTTAACTTCTACCACAAGTGATCAGCAAAACCACCTCCACCACAACCTACACAATCAGCCACTGCCTCCTCCTTGTTTTGAGTTCTACAGCTTCGGCGATTATGAACGGCCCCGCGTACT
TARGET=461,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2645
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=0.132262
PRIMER_LEFT_SEQUENCE=TGAAGGACACTCATATGCCAAG
PRIMER_RIGHT_SEQUENCE=TTATTGGAGATCACAGTGGCAG
PRIMER_LEFT=337,22
PRIMER_RIGHT=521,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=60.132
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=0.2645
PRIMER_LEFT_1_PENALTY=0.132262
PRIMER_RIGHT_1_PENALTY=0.132262
PRIMER_LEFT_1_SEQUENCE=CTGAAGGACACTCATATGCCAA
PRIMER_RIGHT_1_SEQUENCE=TTATTGGAGATCACAGTGGCAG
PRIMER_LEFT_1=336,22
PRIMER_RIGHT_1=521,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=60.132
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=0.3867
PRIMER_LEFT_2_PENALTY=0.254467
PRIMER_RIGHT_2_PENALTY=0.132262
PRIMER_LEFT_2_SEQUENCE=CAGGGTACCGATCACTAGGGTA
PRIMER_RIGHT_2_SEQUENCE=TTATTGGAGATCACAGTGGCAG
PRIMER_LEFT_2=297,22
PRIMER_RIGHT_2=521,22
PRIMER_LEFT_2_TM=60.254
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=0.4132
PRIMER_LEFT_3_PENALTY=0.332305
PRIMER_RIGHT_3_PENALTY=0.080887
PRIMER_LEFT_3_SEQUENCE=GTTGACAGTTCCTGCATACGAC
PRIMER_RIGHT_3_SEQUENCE=ACTGTTATAAGCAGGGCCACAT
PRIMER_LEFT_3=429,22
PRIMER_RIGHT_3=683,22
PRIMER_LEFT_3_TM=59.668
PRIMER_RIGHT_3_TM=59.919
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.4542
PRIMER_LEFT_4_PENALTY=0.373358
PRIMER_RIGHT_4_PENALTY=0.080887
PRIMER_LEFT_4_SEQUENCE=GACAGTTCCTGCATACGACATC
PRIMER_RIGHT_4_SEQUENCE=ACTGTTATAAGCAGGGCCACAT
PRIMER_LEFT_4=432,22
PRIMER_RIGHT_4=683,22
PRIMER_LEFT_4_TM=59.627
PRIMER_RIGHT_4_TM=59.919
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_03672 Ps_contig_mira-and-tgicl-ass_franssen_30671
SEQUENCE=CAACTTCAACACAGTAGCTTATTAAGGTGGCGGGATGATGCATAATAAAAACCATGCTTCTTACATGCAGCAAAATCTTATTCCTAGCTCAAGCACAATCATAAGCACTTATAGCCTATATATTGGCATCGTGATCCATTGTCGTGACTCTACCGAGTTCAGAGTAAGAGAAAACACATACAACACCGATATTCAACAATCAGTCCTCAAACAGATAATCATAGAAAAACAATATAAGTTATGACCATAATCCATAACCTGTAATCCACTAGTATCTCCTACTAGGGTAGCGATCACCAGGGTACCGATCACTAGGGTAGCGATCCATAAAGCGACCTGAAGGACACTCATATGCCAAGTGACCACGGCCTCCACAATTGTGACAAATCATCAAGGGGCCCATGCAGTCACGGCTCATATGACCCAACTGTTGACAGTTCCTGCATACGACATCACGGTAACCACCACCACCCGCTCCACCAGCACCACGGAAGCTGCCTCTGCCACTGTGATCTCCAATAATGTTTGATTTTGGACATTGTCTAGCCACATGTCCAGAAACATTGCAGACATTGCAAATTGGGTCATTTGGACAATCACGTGCCAAGTGCCCTGTTTTCCTACAGTTATTGCAAGCCTTCTCATTCGTGCATTCAACAGCAATGTGGCCCTGCTTATAACAGTTATGGCACAACCTTAAGTCCCCTGGGGGCATTGCTGGAGCTGAGCACTCCCTGGCACGGTGGCCAGTTTTACCACAGGTGTGGCAAATGCCCTCATTTGGACAACTGCTGGCCATGTGGCCAGGTTCTTTGCAATTCCAGCACACTGATTTTGTGGAGCATTCAGAAGCAATGTGCCCAGGAAGGCCACAATTGTGGCAAACTGCAACATTAGGGCACTCTCTAGCATAGTGACCAGGTCTCTTGCAGTTCTTGCACAAATTGTCTCGGCTGAAACCCCGACTTGAATCTCTCCTGTAAGGAGCATCACGATAGGAATGTCGATCAGAGCGAATCTTACGGATCCCTGGGCTACGACTCCTACTCCTGCTTCGGCTTCGGCTGCGGCTGTCTGAACTCATTTTCACTTTCTTTCTTTCTTAACTTCTACCACAAGTGATCAGCAAAACCACCTCCACCACAACCTACACAATCAGCCACTGCCTCCTCCTTGTTTTGAGTTCTACAGCTTCGGCGATTATGAACGGCCCCGCGTACT
TARGET=1090,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2265
PRIMER_LEFT_PENALTY=0.010294
PRIMER_RIGHT_PENALTY=0.216250
PRIMER_LEFT_SEQUENCE=AGGAGCATCACGATAGGAATGT
PRIMER_RIGHT_SEQUENCE=ATAATCGCCGAAGCTGTAGAAC
PRIMER_LEFT=982,22
PRIMER_RIGHT=1203,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=59.784
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.3120
PRIMER_LEFT_1_PENALTY=0.095771
PRIMER_RIGHT_1_PENALTY=0.216250
PRIMER_LEFT_1_SEQUENCE=TCACGATAGGAATGTCGATCAG
PRIMER_RIGHT_1_SEQUENCE=ATAATCGCCGAAGCTGTAGAAC
PRIMER_LEFT_1=989,22
PRIMER_RIGHT_1=1203,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.784
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=215
PRIMER_PAIR_PENALTY_2=0.3362
PRIMER_LEFT_2_PENALTY=0.010294
PRIMER_RIGHT_2_PENALTY=0.325919
PRIMER_LEFT_2_SEQUENCE=AGGAGCATCACGATAGGAATGT
PRIMER_RIGHT_2_SEQUENCE=CTGATTGTGTAGGTTGTGGTGG
PRIMER_LEFT_2=982,22
PRIMER_RIGHT_2=1158,22
PRIMER_LEFT_2_TM=59.990
PRIMER_RIGHT_2_TM=60.326
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=0.4033
PRIMER_LEFT_3_PENALTY=0.077340
PRIMER_RIGHT_3_PENALTY=0.325919
PRIMER_LEFT_3_SEQUENCE=GACCAGGTCTCTTGCAGTTCTT
PRIMER_RIGHT_3_SEQUENCE=CTGATTGTGTAGGTTGTGGTGG
PRIMER_LEFT_3=915,22
PRIMER_RIGHT_3=1158,22
PRIMER_LEFT_3_TM=59.923
PRIMER_RIGHT_3_TM=60.326
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.4215
PRIMER_LEFT_4_PENALTY=0.095538
PRIMER_RIGHT_4_PENALTY=0.325919
PRIMER_LEFT_4_SEQUENCE=TGCAACATTAGGGCACTCTCTA
PRIMER_RIGHT_4_SEQUENCE=CTGATTGTGTAGGTTGTGGTGG
PRIMER_LEFT_4=886,22
PRIMER_RIGHT_4=1158,22
PRIMER_LEFT_4_TM=59.904
PRIMER_RIGHT_4_TM=60.326
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_03673 Ps_contig_mira-and-tgicl-ass_franssen_30679
SEQUENCE=ATTTTGATGGCATTAACACACTTCCATTTCCACCACCTCCATTTTCTGACGAAATAACCAACCAACCAACCATGCTCACTTCACCTTTACTCTCCGTCTGTAACCACACCTTTTCCTACAACCCCATCAACTACCATCACCTCCCACCTTTAAAGTTTCAATCTTTAGCTTTAGGAAGATACGGAAGAGCTGAATTTTCATCTGGGTCATGCCCTAATGATCGTAGAAGAAAAAGGGTGTTGGTGAAAGGGAGTGTGAAGTTGGAGGAAGGAGCTGTGGAAAGTGGGAAGAAAGTGAATTGGGGTGAAGGGTTTGATGCAATTGTTATTGGGTCTGGGATTGGTGGGTTGGTTGCTGGGACTCAGTTGGCTGTGAAAGGTGCTAGAGTTTTGGTTTTGGAGAAGTATGTTATTCCCGGTGGAAGCTCTGGGTTTTATCAAAGGGATGGTTATACTTTTGATGTTGGCTCTTCTGTCATGTTTGGTTTCAGTGATAAGGGTAATCTCAATTTGATAACACAAGCATTGGAGGCAGTTGGCTGTCGGATGGAAGTAATACCTGATCCAACCACTGTTCATTTCCATCTACCCAACCACCTCTCAGTTCGAGTGCATAAAGAATATGTTAAGTTTATCGAAGAATTGACCAATTATTTTCCCCATGAAAAGGATGGCATCCTCAAATTCTATGGCGAATGCTGGAAGATTTTCAATGCCTTGAATTCGTTGGAGTTGAAGTCGCTAGAGGAGCCACTCTACCTTTTCGGACAGTTTTTTCAGAAGCCGCTCGAATGCTTGACATTAGCCTATTACTTACCACAAAATGCTGGAGCGATAGCCCGAAAGTATATTCAGGATCCACAGTTGTTGTCTTTCATAGATGCAGAGTGTTTTATAGTGAGCACTGTCAACGCTTTGCAGACTCCAATGATCAATGCAAGCATGGTTCTTTGTGACAGACACTTTGGTGGGATTAACTATCCTGTTGGGGTGTTGGTGGCATTGCAAAGTCGTTGGCAAAAGGTCTAATTGATCAGGGTAGTCAGATACTTTACAAGGCAAATGTCACGAGTATTATCACCGAGCAGGGCAAAGCAGTAGGAGTGAGGCTTTCCGATGGCAGAGAGTTCTTTGCCAAAACTATAATATCAAATGCTACCAGATGGGACACATTTGGAAAGTTAATAAAAGAAGAATCACTTCCAAAAGAGGAAGAGAACTTCCAGAAAGTTTATGTTAAAGCTCCATCTTTTCTTTCAATCCACATGGGGGTTAAAGCAGAAGTTTTGCCACTAGATACAGATTGTCACCATTTTGTGCTTGAGGACAATTGGACCAAATTGGAAGAACCTTACGGAAGTATCTTTTTGAGTATACCAACTATACTCGATTCTTCATTAGCTCCTGAAGGTCGTCACATCCTTCATATATTCACAACTTCTTCCATGGACGACTGGAAGGGTCTCTCGAGAATTGAATACGAGGCAAAGAAACAGGTGGTAGCAGATGAAATCATAAGCAGATTAGAGAAGAATTTGTTTCCAGGTCTTAAATCATCTATCGAATTTATAGAGGTAGGGACACCAAAGACACATCGACGATATCTAGCTCGTGACGAGGGTACTTATGGACCAATGCCACGCAGCATTCCGAAGGGGTTATTAGGAATGCCGTTTAACACGACAAGCATAGACGGTCTTTACTGTGTTGGAGACAGTTGCTTCCCTGGCCAAGGTGTTATTGCTGTAGCTTTCTCAGGAGTTATGTGTGCTCATCGAGTAGCTGCAGATATTGGGTTGGAGAAAAAGTCACCTGTTTTAGACGCCATGCTGCTTCGGTTGCTCGGTTGGTTCAGGACACTGGCATGAGTTTTATGATTTGTTTGATCCGCGAGTCTATGTATTATCATGTATAACACTACCATTTTTTTTATAGTTTCTTTATTTTTTGTCGAAGGATGAAACCAGATTCCTCATCTTTACAGTTATTTTAGGGCATCCGATCAAACAACTTACATTCTGAGATGTATTTTAAAGCAAATTTTGTGAAGTTGGACCCATCGCAATTCAGCGGCCAAGATTGCTTAGCTGCGTCAGTTGCGTGGTTCTATAAGTGCAAAAGAATCCAAAAGTAAGGAGTGAGGGGTTGTGAAGTGTAAAATGTAAATGATCTCTGTATGTTCAGTTTTCTTTTATCCCAACAGATTTATACTTATACAAGATATTCTTGCTAGTG
TARGET=56,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1450
PRIMER_LEFT_PENALTY=0.132857
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TTGATGGCATTAACACACTTCC
PRIMER_RIGHT_SEQUENCE=TCACCAACACCCTTTTTCTTCT
PRIMER_LEFT=3,22
PRIMER_RIGHT=245,22
PRIMER_LEFT_TM=59.867
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.1614
PRIMER_LEFT_1_PENALTY=0.132857
PRIMER_RIGHT_1_PENALTY=0.028536
PRIMER_LEFT_1_SEQUENCE=TTGATGGCATTAACACACTTCC
PRIMER_RIGHT_1_SEQUENCE=CCAACACCCTTTTTCTTCTACG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=242,22
PRIMER_LEFT_1_TM=59.867
PRIMER_RIGHT_1_TM=60.029
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=0.2424
PRIMER_LEFT_2_PENALTY=0.132857
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=TTGATGGCATTAACACACTTCC
PRIMER_RIGHT_2_SEQUENCE=GGTAGTTGATGGGGTTGTAGGA
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=134,22
PRIMER_LEFT_2_TM=59.867
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=0.2746
PRIMER_LEFT_3_PENALTY=0.262407
PRIMER_RIGHT_3_PENALTY=0.012153
PRIMER_LEFT_3_SEQUENCE=TAACACACTTCCATTTCCACCA
PRIMER_RIGHT_3_SEQUENCE=TCACCAACACCCTTTTTCTTCT
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=245,22
PRIMER_LEFT_3_TM=60.262
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.2764
PRIMER_LEFT_4_PENALTY=0.132857
PRIMER_RIGHT_4_PENALTY=0.143555
PRIMER_LEFT_4_SEQUENCE=TTGATGGCATTAACACACTTCC
PRIMER_RIGHT_4_SEQUENCE=AGATGAAAATTCAGCTCTTCCG
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=202,22
PRIMER_LEFT_4_TM=59.867
PRIMER_RIGHT_4_TM=59.856
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_03674 Ps_contig_mira-and-tgicl-ass_franssen_30682
SEQUENCE=CTAAAGAAGGAAGGGGGGAAAGGAGGAAAAAAAAAGGAAAAAAAATTTTAACGGGGGTAAAAATTAGGAAATTTTTGNAAAAAAAACCTAAATTTGATCGTTTTTGTTGAGGAATTGAGATGGCTCAGGTTCAGGTTCAGCCTCAGAATGCGATGTCGGGCCCGAACGGCGGTGCTGCTGCGGCGGCAGCAGCCGCTGCAGCAGCGGCTGGTGGGAATCAGTTTGTGACGACTTCGCTTTACGTTGGAGATCTTGATGTCAATGTTACGGATTCTCAGCTTTATGACCTTTTCAATCAATTGGGTCAGGTTGTGTCTGTTAGGGTTTGCAGGGATTTGACCACTAGGAGGTCACTTGGTTATGGTTATGTTAACTATAGCAACCCTCAAGATGCTGCCAGAGCATTGGATGTTCTGAACTTTACTCCTCTGAACAACAGGCCCATTCGTATTATGTATTCACACCGTGACCCCAGTATCCGCAAAAGTGGTCAAGGAAATATATTTATTAAGAATTTGGACAAGGCAATTGACCATAAGGCATTGCATGATACATTTTCTACATTTGGGAGTATCCTTTCTTGCAAGGTTGCTGTGGATGGATCTGGCCAATCAAAAGGCTACGGCTTTGTTCAGTTTGATAGTGAGGAGGCTGCTCAAAAAGCTATTGAAAAGCTTAATGGTATGCTGTTGAATGATAAGCAAGTTTATGTGGGACCCTTCCTTCGCAAGCAAGAGAGAGAGACCACAACTGACAGGGCAAAATTCAACAATGTTTTTGTTAAGAATCTATCGGAAACAACCACCGACGATGAGTTGAAGCAGATTTTTGGGGAATTTGGAACAATTACTAGTGCTGTAGTAATGAGGGATGGGGATGGGAAATCAAAGTGTTTTGGATTTGTAAACTTTGAGAGTACCGATGATGCTGCTAGGGCTGTTGAGGCTCTTAATGGGAAAAAGATTGATGACAAGGAATGGTATGTTGGAAAAGCTCAAAAGAAATCTGAAAGGGAGCATGAACTGAAACAAAAATTTGAGCAGAGCATGAAAGAAGCTGCTGATAAATATCAAGGGGCAAACCTGTATGTCAAAAATTTGGATGATAGCATTGTTGATGACAAACTTAAGGAGCTGTTCTCCTCTTTTGGTACAATTACCTCTTGCAAGGTAATGAGAGACCCCAATGGCATAAGTCGAGGATCTGGGTTCGTTGCATTCTCTACTCCTGAGGAGGCATCTAGAGCACTCTTGGAGATGAATGGCAAAATGGTGGTAAGCAAACCTTTGTATGTGACTCTGGCCCAACGGAAAGAAGATAGAAGAGCTAGGCTGCAGGCTCAATTTGCTCAAATGCGTCCTGTTGCGATGCCCCCATCTGTTGCTCCTCGCATGCCTATGTATCCCCCTGGTGGTCCAGGTATGGGTCAACAAATATTTTATGGTCAAGGCCCTCCTGCCATCATTCCTTCCCAGCCTGGATTTGGGTATCAACAACAACTTGTGCCTGGAATGAGGCCTGGTGGGGGTCCTGTGCCAAATTTCTTTGTGCCAATGGTTCAGCAGGGACAGCAAGGGCAGCGTCCTGGTGGAAGACGTGGAGGTGCAGTCCAGCAGTCCCAGCAGCCAGTTCCTCTTATGCCACAACAGATGCTTCCTAGGGGGCGTGTGTATCGCTATCCTTCTGGTAGGGGCATGCCTGATGGTCCCATGCCTGGAGTTGCTGGAGGCATGTATTCTGTTCCTTATGATGTGGGTGGGATGCCCCTTCGTGATGCATCACTTTCACAACAAATTCCCATTGGTGCTTTGGCTTCTCATCTGGCTAATGCATCTCCAGAGCAACAGAGGACGATGCTGGGTGAGAATCTTTACCCACTTGTGGAACAGTTGGAGCCCGACAATGCTGCTAAAGTTACTGGAATGCTTCTGGAGATGGACCAGACTGAAGTTCTGCACTTGCTTGAGTCACCAGAAGCCCTGAAAGCAAAGGTGGCAGAGGCAATGGACGTCCTCAGGAATGTTGCACAGCAGCAGGCTGGCGGTGCTGCTGATCAGTTGTCTTCATTGTCCCTTAATGACAGCCTTGTTTCTTAAGTTATTTATGCTGCTGGGATTTACTTCCTTGGTGAATGACTATTTATCTTTACCGGCACTGGCTTTTATCCGAATTTCAGATTGAGTTGTTTTATTTACTAGACTAGGTGTTTTCAGTTTTTGGATCTCCTTCCTTTGGTTGAAGGCATGTTTGATTGCTGATATTTATTATTATGACTTGAATTTGGAACATAAAATTGTTTGTTTGGATTGCTG
TARGET=734,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2945
PRIMER_LEFT_PENALTY=0.108707
PRIMER_RIGHT_PENALTY=0.185822
PRIMER_LEFT_SEQUENCE=AGTTTATGTGGGACCCTTCCTT
PRIMER_RIGHT_SEQUENCE=CCTTGTCATCAATCTTTTTCCC
PRIMER_LEFT=703,22
PRIMER_RIGHT=974,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=59.814
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.2945
PRIMER_LEFT_1_PENALTY=0.108707
PRIMER_RIGHT_1_PENALTY=0.185822
PRIMER_LEFT_1_SEQUENCE=AAGTTTATGTGGGACCCTTCCT
PRIMER_RIGHT_1_SEQUENCE=CCTTGTCATCAATCTTTTTCCC
PRIMER_LEFT_1=702,22
PRIMER_RIGHT_1=974,22
PRIMER_LEFT_1_TM=60.109
PRIMER_RIGHT_1_TM=59.814
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.3091
PRIMER_LEFT_2_PENALTY=0.108707
PRIMER_RIGHT_2_PENALTY=0.200343
PRIMER_LEFT_2_SEQUENCE=AGTTTATGTGGGACCCTTCCTT
PRIMER_RIGHT_2_SEQUENCE=TCCCCATCCCTCATTACTACAG
PRIMER_LEFT_2=703,22
PRIMER_RIGHT_2=876,22
PRIMER_LEFT_2_TM=60.109
PRIMER_RIGHT_2_TM=60.200
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.3091
PRIMER_LEFT_3_PENALTY=0.108707
PRIMER_RIGHT_3_PENALTY=0.200343
PRIMER_LEFT_3_SEQUENCE=AAGTTTATGTGGGACCCTTCCT
PRIMER_RIGHT_3_SEQUENCE=TCCCCATCCCTCATTACTACAG
PRIMER_LEFT_3=702,22
PRIMER_RIGHT_3=876,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=60.200
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.3361
PRIMER_LEFT_4_PENALTY=0.108707
PRIMER_RIGHT_4_PENALTY=0.227356
PRIMER_LEFT_4_SEQUENCE=AGTTTATGTGGGACCCTTCCTT
PRIMER_RIGHT_4_SEQUENCE=TCCAACATACCATTCCTTGTCA
PRIMER_LEFT_4=703,22
PRIMER_RIGHT_4=988,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=60.227
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_03675 Ps_contig_mira-and-tgicl-ass_franssen_30684
SEQUENCE=GTCGGGACAACGTTCATCTTACGCTAACATTCAATTCAATACTTTCCGATTTTTTCTAAAATCTCTCCTTTTCTTCTTCTTCAACCCTCATTCTCAATCATTCGTTCTTCTTCCACGCTCCCGACTCAGTTTCCATTATCATGGCTTCGAAACGTATCTTGAAGGAGCTCAAGGATTTGCAGAAGGATCCTCCAACGTCATGCAGTGCAGGTCCTGTGGCTGAGGACATGTTCCACTGGCAAGCCACGATCATGGGACCGTCTGATAGTCCATATACAGGCGGTGTTTTCCTGGTTTCGATACATTTTCCTCCTGACTATCCTTTCAAGCCACCAAAGGTTGCTTTTAGGACCAAGGTATTCCACCCAAACATCAACAGCAACGGTAGCATATGTCTCGACATTCTCAAAGAACAGTGGAGTCCAGCACTAACCATTTCCAAGGTCCTTTTGTCAATTTGCTCATTGTTGACGGACCCCAACCCTGACGATCCACTTGTGCCTGAAATTGCGCACATGTACAAGACGGACAGGGCCAAGTACGAGGCCACAGCCCGCAGCTGGACACAGAAGTATGCCATGGGATGATCATGCATGTCTGAGCTGTTCAATTAAAACAGCAATGTCATAATTAATGTAAAAGAGCAACAGATATTTTGAATGAATTTTAATGCTTTATTGTTTAGTGGAACTTAAAGCTGGCAAGGGATCCCAGTTATCATTTGTGAATATTGCATTGTAAGACTCCTGTGATTGAGGAAACTGTAATGTTATGCAATCTATCTGCTTGTTGGTACAACAAGTATTAGACGACAAAAGATACAAGGGTCACAAATTAAGCAGTGACTTCTTACATCGGTGAACAGATTCGGATTCTGTTCTAGATTGTTAAATGAAAGATCAGATCACTTGTA
TARGET=71,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7132
PRIMER_LEFT_PENALTY=0.706271
PRIMER_RIGHT_PENALTY=0.006951
PRIMER_LEFT_SEQUENCE=GGACAACGTTCATCTTACGCTA
PRIMER_RIGHT_SEQUENCE=AGATACGTTTCGAAGCCATGAT
PRIMER_LEFT=4,22
PRIMER_RIGHT=158,22
PRIMER_LEFT_TM=59.294
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.2000
PRIMER_RIGHT_END_STABILITY=6.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.7893
PRIMER_LEFT_1_PENALTY=0.782312
PRIMER_RIGHT_1_PENALTY=0.006951
PRIMER_LEFT_1_SEQUENCE=GTCGGGACAACGTTCATCTTAC
PRIMER_RIGHT_1_SEQUENCE=AGATACGTTTCGAAGCCATGAT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=158,22
PRIMER_LEFT_1_TM=60.782
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.8423
PRIMER_LEFT_2_PENALTY=0.706271
PRIMER_RIGHT_2_PENALTY=0.135999
PRIMER_LEFT_2_SEQUENCE=GGACAACGTTCATCTTACGCTA
PRIMER_RIGHT_2_SEQUENCE=AAAACACCGCCTGTATATGGAC
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=59.294
PRIMER_RIGHT_2_TM=60.136
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.8735
PRIMER_LEFT_3_PENALTY=0.706271
PRIMER_RIGHT_3_PENALTY=0.167242
PRIMER_LEFT_3_SEQUENCE=GGACAACGTTCATCTTACGCTA
PRIMER_RIGHT_3_SEQUENCE=ACGAATGATTGAGAATGAGGGT
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=104,22
PRIMER_LEFT_3_TM=59.294
PRIMER_RIGHT_3_TM=59.833
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.2000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=0.9140
PRIMER_LEFT_4_PENALTY=0.706271
PRIMER_RIGHT_4_PENALTY=0.207756
PRIMER_LEFT_4_SEQUENCE=GGACAACGTTCATCTTACGCTA
PRIMER_RIGHT_4_SEQUENCE=GATCCTTCTGCAAATCCTTGAG
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=188,22
PRIMER_LEFT_4_TM=59.294
PRIMER_RIGHT_4_TM=60.208
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_03676 Ps_contig_mira-and-tgicl-ass_franssen_30688
SEQUENCE=AAGACCAACAAAAAACAAAGCAAAAGAGAAAAAAAAAGAAAAGAAAAGATAGCGAGAGGGGAATTCGAGGAAGAAGAAGAACAAGGGTTTGGTTGAAGACGAAGATGAGTGAACAACAGAGTAACAGCAATAACCAGCTGGTAGTGCAGAATTCAGGGAGTTTAAGCTTCAGTAGTCACTTGTCTAAGGAAGATGAAGAGATGTCTAGGTCTGCTCTTTCAACTTTTAGGGCTAAAGAAGAAGAAATCGAGAAGAAGAAAATGGAAGTCAGAGAGAAAGTTCAGTTTCAGCTAGGTCGTGTTGAAGAAGAAACCAAACGTCTCGCTACAATTCGTGAGGAGCTTGAAGCACTTGCAGATCCAATGAGAAAAGAAGTTTCAGTTGTTAGAAAGAAAATTGATTCAGTAAACAAAGAATTAAAGCCACTTGGTCAATCTTGCCAGAAGAAGGAGAAAGAATACAAAGATGCCCTTGAAGCTTTTAATGATAAAAACAGGGAAAAAGTACAGTTAATTACAAAATTAATGGAGTTGGTGGGTGAAAGTGAAAGATTGAGGATGAAGAAGCTGGAGGAGCTGAGTAAGAACATAGATTCAATGCAAGTGCAATGATTTATTAGCCAACTGATGATTAGTGAATCCTGTGTGGTATATTAAAGATGATGACATAGTATTTTTATATGTATGGGTGTCTTGTGATTTTGTAATTTTGTGTTTTAACTAGCATTTGATTTCATATGGGATATTTTGCTAATTTAGGAGGGTTATTATACCCTAGAAAACAGGCTTGTAACTAAATATGTTGCATTTTTATTCACTTTTCTATGCTTGTTCCTTCTTACTATTTAATTTATTCATGTAATTTGAATTTTAGTT
TARGET=34,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03677 Ps_contig_mira-and-tgicl-ass_franssen_30688
SEQUENCE=AAGACCAACAAAAAACAAAGCAAAAGAGAAAAAAAAAGAAAAGAAAAGATAGCGAGAGGGGAATTCGAGGAAGAAGAAGAACAAGGGTTTGGTTGAAGACGAAGATGAGTGAACAACAGAGTAACAGCAATAACCAGCTGGTAGTGCAGAATTCAGGGAGTTTAAGCTTCAGTAGTCACTTGTCTAAGGAAGATGAAGAGATGTCTAGGTCTGCTCTTTCAACTTTTAGGGCTAAAGAAGAAGAAATCGAGAAGAAGAAAATGGAAGTCAGAGAGAAAGTTCAGTTTCAGCTAGGTCGTGTTGAAGAAGAAACCAAACGTCTCGCTACAATTCGTGAGGAGCTTGAAGCACTTGCAGATCCAATGAGAAAAGAAGTTTCAGTTGTTAGAAAGAAAATTGATTCAGTAAACAAAGAATTAAAGCCACTTGGTCAATCTTGCCAGAAGAAGGAGAAAGAATACAAAGATGCCCTTGAAGCTTTTAATGATAAAAACAGGGAAAAAGTACAGTTAATTACAAAATTAATGGAGTTGGTGGGTGAAAGTGAAAGATTGAGGATGAAGAAGCTGGAGGAGCTGAGTAAGAACATAGATTCAATGCAAGTGCAATGATTTATTAGCCAACTGATGATTAGTGAATCCTGTGTGGTATATTAAAGATGATGACATAGTATTTTTATATGTATGGGTGTCTTGTGATTTTGTAATTTTGTGTTTTAACTAGCATTTGATTTCATATGGGATATTTTGCTAATTTAGGAGGGTTATTATACCCTAGAAAACAGGCTTGTAACTAAATATGTTGCATTTTTATTCACTTTTCTATGCTTGTTCCTTCTTACTATTTAATTTATTCATGTAATTTGAATTTTAGTT
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0893
PRIMER_LEFT_PENALTY=0.062568
PRIMER_RIGHT_PENALTY=0.026744
PRIMER_LEFT_SEQUENCE=GAAAAGATAGCGAGAGGGGAAT
PRIMER_RIGHT_SEQUENCE=CAACACGACCTAGCTGAAACTG
PRIMER_LEFT=42,22
PRIMER_RIGHT=302,22
PRIMER_LEFT_TM=60.063
PRIMER_RIGHT_TM=59.973
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.1148
PRIMER_LEFT_1_PENALTY=0.062568
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=GAAAAGATAGCGAGAGGGGAAT
PRIMER_RIGHT_1_SEQUENCE=TTCTTCAACACGACCTAGCTGA
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=307,22
PRIMER_LEFT_1_TM=60.063
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.1244
PRIMER_LEFT_2_PENALTY=0.062568
PRIMER_RIGHT_2_PENALTY=0.061818
PRIMER_LEFT_2_SEQUENCE=GAAAAGATAGCGAGAGGGGAAT
PRIMER_RIGHT_2_SEQUENCE=ATTGTAGCGAGACGTTTGGTTT
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=330,22
PRIMER_LEFT_2_TM=60.063
PRIMER_RIGHT_2_TM=60.062
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.2372
PRIMER_LEFT_3_PENALTY=0.062568
PRIMER_RIGHT_3_PENALTY=0.174657
PRIMER_LEFT_3_SEQUENCE=GAAAAGATAGCGAGAGGGGAAT
PRIMER_RIGHT_3_SEQUENCE=GTAGCGAGACGTTTGGTTTCTT
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=327,22
PRIMER_LEFT_3_TM=60.063
PRIMER_RIGHT_3_TM=59.825
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=0.2381
PRIMER_LEFT_4_PENALTY=0.211367
PRIMER_RIGHT_4_PENALTY=0.026744
PRIMER_LEFT_4_SEQUENCE=AAAGAAAAGATAGCGAGAGGGG
PRIMER_RIGHT_4_SEQUENCE=CAACACGACCTAGCTGAAACTG
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=302,22
PRIMER_LEFT_4_TM=60.211
PRIMER_RIGHT_4_TM=59.973
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_03678 Ps_contig_mira-and-tgicl-ass_franssen_30693
SEQUENCE=CGTTAATGTTAAAGTAGGAATATAAATCAACTTTATTGTTCTTACATATGACAATATAACAATGATACACAAAACTGAAAGATACCAACAAATCGTTCTACAACAAATACACCAATGAAAACAGCCAAATAAATAAGAAACTAAAAATGGTAATAACGAAATGATGCTTATTAATCATACCCAACCAAGTAAACATAGCAATCCCCGAAAAATTTATTACAACATAATCATACCGCTAATTCATGTTTCAACCCAAAATGTTTCCGTAACACAAAAATACAGCACCGACAACAACTGCAACTGAGTACACCAGAGACCTCAAAGGCGCTATTGAATTAGATGACGCCGGTGGAACCGCTGCTCCTCCAGGTGCCAGAGACTGTGCACCCGATGCCGCAGCACCATCTGCTGGAATAGTTGGAGATGGAGATGGCGGTGAAGCTTCCGTGGAAGGAGAGATCGGTGATGGAGCAGGTGGTCCACCTGCCGTAGGAGGTGGAGCCGTAGGTTCACCGGCGGGAGGCATACTGAAGGATGGTGATAGAGAGGGTGGATTTGCAGGGGGAGAAGCCGAAAGAGAGGGAGACGGCGAAGGAGCCGATTTTGGTGTGTGGCCTCGTGGGGATATAACGACAAGGGTTAATTTCTGACCCTTTTCACAATTTTTATCATTTCCACTAATGAAATAGAATGGTCCAGATCGATCCAAAGTAAACTCTGTGTTACCATCTTCAAACTTGTTGATTGGGTTTGTTTTGTTGCATTTATCATAAGCTTCTTTGTTCACTTCCAACACAGAGTCTGAACCCTTGTTGTACTTGAAAACTAGAATGTCGTTGACTAGGAACCTGTTTCTTCTGGACCATTGGTTGTAATTCTCGGGAGGGTTTAAAGACCATCCTGCGGTTCTGCCGATATCAAATTTGAGGGCTTGGGAAGTTGAGACAAGAACAAACAAGAGAAAGAGAGGTATCTTAAAATTCATAGTAAGGTGTGTAGAGGGGAGGCTATGAAAGGATTGAGGGGGGTGTCTAGTATATAATCCGAATTAGAGAGAGAGAGAAAGTAGAAGTGTGTGTTGTGACACACGATGTATGTCTGATAAAGGAAATTGAATGGAAGAAACAAATGGGGCGACTTGGATAAGACTTTTCAAAAGGAAAGTTGAGAGTTGAGGTGTGATATAGACGAAATGCGAAGTCTTAACGTGTGTTGATTATTCGCTTTCATTCACTAAGTTCTTATATTCACAACCATACACTTGGCAAAGT
TARGET=1051,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3230
PRIMER_LEFT_PENALTY=0.162220
PRIMER_RIGHT_PENALTY=0.160807
PRIMER_LEFT_SEQUENCE=TTGTAATTCTCGGGAGGGTTTA
PRIMER_RIGHT_SEQUENCE=CGTGTGTCACAACACACACTTC
PRIMER_LEFT=869,22
PRIMER_RIGHT=1089,22
PRIMER_LEFT_TM=59.838
PRIMER_RIGHT_TM=60.161
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.3230
PRIMER_LEFT_1_PENALTY=0.162220
PRIMER_RIGHT_1_PENALTY=0.160807
PRIMER_LEFT_1_SEQUENCE=TTGTAATTCTCGGGAGGGTTTA
PRIMER_RIGHT_1_SEQUENCE=TCGTGTGTCACAACACACACTT
PRIMER_LEFT_1=869,22
PRIMER_RIGHT_1=1090,22
PRIMER_LEFT_1_TM=59.838
PRIMER_RIGHT_1_TM=60.161
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.3578
PRIMER_LEFT_2_PENALTY=0.197024
PRIMER_RIGHT_2_PENALTY=0.160807
PRIMER_LEFT_2_SEQUENCE=CAACACAGAGTCTGAACCCTTG
PRIMER_RIGHT_2_SEQUENCE=CGTGTGTCACAACACACACTTC
PRIMER_LEFT_2=790,22
PRIMER_RIGHT_2=1089,22
PRIMER_LEFT_2_TM=59.803
PRIMER_RIGHT_2_TM=60.161
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.3578
PRIMER_LEFT_3_PENALTY=0.197024
PRIMER_RIGHT_3_PENALTY=0.160807
PRIMER_LEFT_3_SEQUENCE=CACAGAGTCTGAACCCTTGTTG
PRIMER_RIGHT_3_SEQUENCE=TCGTGTGTCACAACACACACTT
PRIMER_LEFT_3=793,22
PRIMER_RIGHT_3=1090,22
PRIMER_LEFT_3_TM=59.803
PRIMER_RIGHT_3_TM=60.161
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.4096
PRIMER_LEFT_4_PENALTY=0.248781
PRIMER_RIGHT_4_PENALTY=0.160807
PRIMER_LEFT_4_SEQUENCE=TGGGAAGTTGAGACAAGAACAA
PRIMER_RIGHT_4_SEQUENCE=CGTGTGTCACAACACACACTTC
PRIMER_LEFT_4=932,22
PRIMER_RIGHT_4=1089,22
PRIMER_LEFT_4_TM=59.751
PRIMER_RIGHT_4_TM=60.161
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_03679 Ps_contig_mira-and-tgicl-ass_franssen_30698
SEQUENCE=GATCCTCTTTCTTCCNNNRGACCCTCCTTTTTYCTTCCTTCCTTCGACACTTCTCTTCATCAACTTTCCAACCAAACCTCCATCTTTTCTCTCATTCTCTTATACCCTTTTACACAACTTCTTCAAAACTCTTCCTTTTAACACTCTTTTCAAGAACCACAGTACCAGTGTTTCTTGAATTCCATTCTTGGACTCTTCATGGCTCTCACTCAGTTAAACTCCACTTGCTCAAAACCTCAACTTCACTCATCATCACAGCTCTCATTTCTCTCTAGGACTAGGACTAGGACTCTTCCTAGGCACTATCACTCTACTTTTGCTCCACTTCACAGAACTCAGCATGCTAGAATTTCTTGTTCTGTTGCACCAAATCAAGTGCAGGTTCCAGCTGCACAAACACAGGATCCCAAGGGTAAGCCTGATTGCTATGGTGTCTTCTGCCTTACCTATGATTTGAAGGCTGAAGAGGAGACAAAATCGTGGAAGAAGTTGATTAACATTGCAGTCTCAGGTGCGGCTGGAATGATTTCCAATCATCTACTTTTCAAGCTTGCATCTGGTGAAGTTTTCGGTCCAGATCAACCTATTGCACTGAAATTATTAGGATCAGAAAGGTCAATCCAAGCTCTTGAAGGTGTTGCAATGGAATTGGAGGACTCTTTGTTTCCTTTGCTGAGAGAGGTTGTTATCAGCATTGATCCTTATGAGGTCTTCCAAGATGCAGAATGGGCTTTGCTAATAGGCGCAAAGCCTCGAGGACCTGGAGTGGAGCGTGCAGCTTTGCTAGACATAAACGGGCAGATTTTTGCGGAGCAGGGAAAAGCACTTAATGCTGTCGCATCGCGTAATGCCAAAGTTATAGTTGTGGGAAACCCTTGCAATACCAATGCATTAATATGTTTGAAGAATGCTCCAAATATTCCTGCGAAAAACTTTCATGCTTTAACTCGTTTAGATGAAAACAGGGCAAAATGTCAGCTAGCCCTCAAGGCAGGTGTCTTCTACGATAAAGTTTCGAATATGACAATATGGGGCAACCACTCAACTACTCAAGTCCCCGACTTCTTAAATGCCAGAATCGACGGTTTGCCCGTCAAAGAAGTGATTAAGGATAATAAGTGGTTAGAGGAAGAGTTCACTGAAAAGGTTCAAAAGAGAGGTGGCGTGCTTATTCAAAAATGGGGAAGATCATCTGCTGCATCAACTTCAGTGTCGATAGTTGATGCCATACGATCTTTGATCACTCCTACTCCCGAGGGTGACTGGTTTTCTTCTGGTGTATATACCAACGGAAATCCTTACGGAATAGCTGAAGATATCGTGTTCAGCATGCCATGTAGATCAAAGGGAGATGGTGATTACGAGCTTGTCAATGATGTTATATTTGATGACTACCTAAGACAGAAATTAGCCAAAACAGAAGCTGAGTTATTGGCCGAGAAAAAATGTGTGGCTCACCTAACAGGCGAGGGAATTGCTGTTTGTGATCTTCCTGGTGATACCATGCTCCCAGGAGAAATGTAACAAGATAATATTCTGAACATCAAAGGAGATTTGGAAGTAAGATCATACGAAATTATGTAGAATAACGAGGAGTGCTAGTAATACTCTTTTTTCAACCCTTATTCTAATGTTCACCATCTTCTCGGGTATAACTCAGCCGAGTCTCGCCACTTTATGAGAATCTCCTATCTAACTGAGATCCATGTTGATTTTACTCGAAGAAAGACTCGTGTTAGAGTGAGAGTTGGAAAGAGAGGATTCCTTGCAGTTCAAGATAAATAATATAAATTTCAAAACATTAATTTGACTTGAAAACCTTAAAGCTCATCAACAATGTAATAAGTGGAATTTTGTATAACCTATTTTTAGTTATGAAGCTTGAGATGGGTCGGTATAAATTATTGTGTTATACAAGTTGGGATACAGTTTATGTGTCTTAGTAATACTGATATAACCCAGAAAATTATGAAC
TARGET=34,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03680 Ps_contig_mira-and-tgicl-ass_franssen_30709
SEQUENCE=AAGTTCAAAGTTTAGTAAACATTAGAACAAGATTTGAGATTAAAAAATAGTGATATGTTCTATAGTTTTGTTAAAAAGTATAAAACCAGATATAACTTAATAGGACAGAACTAGATCAAGTTCCACTTCGTTCTCTGCACCAAACACTACCATAGTAATTTTTTCTGCATATTAACACTGCACCAAACTATATACTTCTCTGCTCTTTCAACAGAAACCTATGCTATTCTGCTATTCTTCTAATGCATGCATTATTGATTCCTGAGTGACTCGCTTTCCGAGATTTGATCAATGGTGGTAGGCGAGCCCGACGGAGAAGTAGCTGTCGAAGTTGAATTAGTTGGTGTGTGTCCTTGAACTGCTAAACTAGAAGGCTGCAACAGATTTGCATCAGGAGCCTTCTCCTTAGATGCATTCATGTTAACTTTTATCTTGTTATGAATATTGACATATTCATCGGTTTCATCAAGTATCTCCTCCTGAAGAAGCTCTTCGATTACATCCTCCATCGAAATAACACCAACAACCACTTCACTGGGCGGAAAGACGGGCAGGGGAGCATTATCAAGGTCCAGAATGCAATATGAACAACCTCTGTGTCGCTTTTTAAAAGCCGGCGTTGCTGGTGGACTTTTTTTCACCTGTGAACCGTCAGCCGTCAAAGTATCGCGCGGCTCCAACATCGCACCTTTATCTAAAGGAGCACTTTTTCCTTTGTTCTTACAGCTGTCTTGAAACTCGAGTTGTTCTGAATCTTTAATTTTTTTGTATGTTCCTTTTTTATCGTTTAGATCCCTATATACAACCGCAATATGACTGTGACCCTTCTGAAATTCATTCAGTATATCATACAGAGGCATATTCTCTGAAACACGAGGAATTTTTCTGATCATCATTTTTCTTAGAGGAACTGAAGCCTTTGAATCAACCATAAAGAGATTTTTAACCAGAACAAGTCCCATAATGTTTGTCCGCTCTCCTGCATAAACCGGGACTCGACTATGACCCATTGTCATTATTGAATTCAGGGTCTCCAAATTTAGAGTTGCATCCAAATCAAGGGAAAATGCCTTTGATATGGGAGTCATGGCATCTTTTGCGGTCTTTTCAGTCAATTCAAGCGCTCCGGCTATGATGGTTGTCTCATCGTGCGTTAAATCTCCTCCTTTTCCAGCCTCATTCCCATGGAAATTCACAAAGGTCTTGAGCTCTGCCCTCTTTAAAAGAGCAGCTTTTCCTTTACCTAACATCCAATCAAGCACCTTGCTGATGGGATAAGCGATGGGATAAAATACTACAAGAAGAACACGAACAAGCGGGGCAAGCGTTGCGCCAACCATCAATCCATATCGCGTACAAATTGCTTGCGGCAATATCTCTCCAAAGATGAGGATTAGAGTAACCGAAATCAAAACAGCAGCATAAGGAGGCACAATAGCATCAAGAAAAATCGGAAGAGTCTCCATGGCTAAAGAATTTCCAATCAAAAGAGTACAAAGCAAAAGATGCTGATTCTTAACCACAGGGAATATCTTAGCGGCATGGATACGATGTTGAGGACGACCAGATTTGATGAGAACTTCGAGGTCGACGAGTCCCAAAGACATGAGTCCAAGGGTGAGACCAGCCATGAGACCAGCGAAGCAAACCAATCCAATGATGAGAAGTATGTAGAGCCAGAATTTGGTTTCACAGCAAGGTGCATCAGCGGCCATATCTCTCTCTCTATGGGAAAAGTTTGGTTAGGAAAAGCGCTTTTTCATTGGCGTTTCAGCTTTTCTTCCCCACAAAACCATTAATAGCCAT
TARGET=1717,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2908
PRIMER_LEFT_PENALTY=0.215215
PRIMER_RIGHT_PENALTY=0.075553
PRIMER_LEFT_SEQUENCE=CAGAATTTGGTTTCACAGCAAG
PRIMER_RIGHT_SEQUENCE=GCTATTAATGGTTTTGTGGGGA
PRIMER_LEFT=1677,22
PRIMER_RIGHT=1802,22
PRIMER_LEFT_TM=59.785
PRIMER_RIGHT_TM=60.076
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=126
PRIMER_PAIR_PENALTY_1=0.4073
PRIMER_LEFT_1_PENALTY=0.331757
PRIMER_RIGHT_1_PENALTY=0.075553
PRIMER_LEFT_1_SEQUENCE=ATATCTTAGCGGCATGGATACG
PRIMER_RIGHT_1_SEQUENCE=GCTATTAATGGTTTTGTGGGGA
PRIMER_LEFT_1=1528,22
PRIMER_RIGHT_1=1802,22
PRIMER_LEFT_1_TM=60.332
PRIMER_RIGHT_1_TM=60.076
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.4452
PRIMER_LEFT_2_PENALTY=0.369628
PRIMER_RIGHT_2_PENALTY=0.075553
PRIMER_LEFT_2_SEQUENCE=GATGTTGAGGACGACCAGATTT
PRIMER_RIGHT_2_SEQUENCE=GCTATTAATGGTTTTGTGGGGA
PRIMER_LEFT_2=1549,22
PRIMER_RIGHT_2=1802,22
PRIMER_LEFT_2_TM=60.370
PRIMER_RIGHT_2_TM=60.076
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=0.4481
PRIMER_LEFT_3_PENALTY=0.372557
PRIMER_RIGHT_3_PENALTY=0.075553
PRIMER_LEFT_3_SEQUENCE=ATGCTGATTCTTAACCACAGGG
PRIMER_RIGHT_3_SEQUENCE=GCTATTAATGGTTTTGTGGGGA
PRIMER_LEFT_3=1505,22
PRIMER_RIGHT_3=1802,22
PRIMER_LEFT_3_TM=60.373
PRIMER_RIGHT_3_TM=60.076
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.4682
PRIMER_LEFT_4_PENALTY=0.392624
PRIMER_RIGHT_4_PENALTY=0.075553
PRIMER_LEFT_4_SEQUENCE=GAATTTGGTTTCACAGCAAGGT
PRIMER_RIGHT_4_SEQUENCE=GCTATTAATGGTTTTGTGGGGA
PRIMER_LEFT_4=1679,22
PRIMER_RIGHT_4=1802,22
PRIMER_LEFT_4_TM=60.393
PRIMER_RIGHT_4_TM=60.076
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_03681 Ps_contig_mira-and-tgicl-ass_franssen_30721
SEQUENCE=AAAAGGGATGATCTCCACTGTCACNTACGGNNCAACGACTCTCACTGAAGGAAAAAGAAATAAAAAATACACCAATAGACCTCAATGAAGTCTCTCTTCGGAAGAGAAAAAGAGGAAAAAGAAAGCTTTTCCTGTTCTTTTCCACAACAGACCCTCTTCTTCTTCACGACCACCACCAACGTAACAAATCTCTCTCCTCCGTGATTCTCACCAAACGCCACGTCTACACCACACGAACTACACACCGATACTCCCACCGCCTTCACACCATCAACCTACACAACGAACATCAATCGTTTCCGTGCTAGTCTGTCCGTCCAAACTCCAACCTCCTTCATCGCCGACGAGCACCAAACTTCTCTGCCGCGAGTCGCTTCCCTCCTCCTCCTCGTCGTGCCTCTACTGCTCTCTACCGCCTTACGCCGTAACAAATCCCTCATCACAACCTTCAGCTTGAAACACCATCGTACCCCACCTCTGATTCACCTTATCCGCACCACCTGCAAGGTGTTCGTAAAAACGACAACCACAATAGCATGATTTAGATCTCTAACTGAAGTATGAAATATGTGCTTTCGATTCAATCAGAGTTTCGATGCATCTGATACCCTCAACTGACATCCTGCTAACGTCCTATCAACTGAGTAACTCATTCTCTGCATA
TARGET=378,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2230
PRIMER_LEFT_PENALTY=0.083883
PRIMER_RIGHT_PENALTY=0.139129
PRIMER_LEFT_SEQUENCE=ACAGACCCTCTTCTTCTTCACG
PRIMER_RIGHT_SEQUENCE=TTGTGATGAGGGATTTGTTACG
PRIMER_LEFT=146,22
PRIMER_RIGHT=444,22
PRIMER_LEFT_TM=59.916
PRIMER_RIGHT_TM=59.861
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=0.2410
PRIMER_LEFT_1_PENALTY=0.167063
PRIMER_RIGHT_1_PENALTY=0.073900
PRIMER_LEFT_1_SEQUENCE=CTAGTCTGTCCGTCCAAACTCC
PRIMER_RIGHT_1_SEQUENCE=CATGCTATTGTGGTTGTCGTTT
PRIMER_LEFT_1=304,22
PRIMER_RIGHT_1=538,22
PRIMER_LEFT_1_TM=60.167
PRIMER_RIGHT_1_TM=59.926
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.2422
PRIMER_LEFT_2_PENALTY=0.174518
PRIMER_RIGHT_2_PENALTY=0.067678
PRIMER_LEFT_2_SEQUENCE=ACTACACACCGATACTCCCACC
PRIMER_RIGHT_2_SEQUENCE=TGTCGTTTTTACGAACACCTTG
PRIMER_LEFT_2=236,22
PRIMER_RIGHT_2=524,22
PRIMER_LEFT_2_TM=60.175
PRIMER_RIGHT_2_TM=60.068
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.2600
PRIMER_LEFT_3_PENALTY=0.192310
PRIMER_RIGHT_3_PENALTY=0.067678
PRIMER_LEFT_3_SEQUENCE=AATCGTTTCCGTGCTAGTCTGT
PRIMER_RIGHT_3_SEQUENCE=TGTCGTTTTTACGAACACCTTG
PRIMER_LEFT_3=291,22
PRIMER_RIGHT_3=524,22
PRIMER_LEFT_3_TM=60.192
PRIMER_RIGHT_3_TM=60.068
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.2662
PRIMER_LEFT_4_PENALTY=0.192310
PRIMER_RIGHT_4_PENALTY=0.073900
PRIMER_LEFT_4_SEQUENCE=AATCGTTTCCGTGCTAGTCTGT
PRIMER_RIGHT_4_SEQUENCE=CATGCTATTGTGGTTGTCGTTT
PRIMER_LEFT_4=291,22
PRIMER_RIGHT_4=538,22
PRIMER_LEFT_4_TM=60.192
PRIMER_RIGHT_4_TM=59.926
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_03682 Ps_contig_mira-and-tgicl-ass_franssen_30722
SEQUENCE=AACTNTAGTAANACCATCATCACAATACATAAGCACACAATACTATATTCACAATCAATACAAACTAAATAGCAAACTAATTTCAAATAGCAAAAATTATTTACATTTCTTTAACATATTAAAACTCAAGAATAAAATAATACATTAGAACTCAAACAATATAATTAAAACTACAGAAACCATGCAATAAAAAAAACTTAATAAAACATACATTAAGACTTAAAAGCCAAACATTAAAACTTATATTTAGAATTATACATTTAGCCGTGAAAGCAAACTCTACATCAGAAAGAACCACACCTATTAGAAGCTCTATTTAATCAGTCATCTCAAACTGGTATTTGAACAAAATACATATAATACTTTAAACATTAAACTCGCCTATCGATCATGTTATTAATACGAAAACTAGCTTTAACCTCAAGATCTCAACACAGTAAAATATGTATTCAAATTAAATACATTATAAACAATCAAACTCAATGCCGAATCGAATCGAATCAAAAATTTGAATTATAACACGAATTAATTTTGTAAACAAGACAGCGAAACTCAGGATCAAAATATTACCCGATGAAATGTGTACACGGCTGCTTCACGTCATTGTTGCATAATCAAAAACGTTATGGAATCCGACGATGTAGAAACAAGAACAAAATCAGATTCAAATCTCGGATCAAACTTACAACTTGCACATAAGTTAATGAAAGACAACAACCAAACCGAATATAAAAATGAGTTTCACGAATTTGAAATAGTCGGAAAAAAATAGTTTAGTTAAAGACGTGAGTGTAGCTATTCTTGTGTCCTGAAATCAGAATCACGACGTGTTGGAAGAGAAGATATCCTCCATAGAACCTTCACAATCCTTCTTTTTCCAATCTCTGTCTCCATTCATCTTTGTGGTTTATGTTCCTTCACAGCTGTCATGTATACAGAGAATGAGTTACTCAGTTGATAGGACGTTAGCAGGATGTAAGTTGAGGGTATCAGATGCGCCGAAACTCTGATTGAATCCAATGCACATAATTCATACTTCAGTTAGAGATTTAAATCATGCTATTGTGGTTGTCGTTTTTACGAACACCTTGCAGGTGGTGCGGACAAGGTGAATTGGAGGTGGGGTACGATGGTGTTTCAAGCTGAAGGTTGTGATGAGGGATTTGTTACGGCGTAAGGCGGCAGAGAGCAGTAGAGGTACGACGAGGAGGAGGAGGGAAGTGACTCGCGGCGGAGAAGTTTGGATGGACGGATTAGCATGGCAACGATTGATGTTCGTCGTGTAGGTCGATGGTGTGAAGGCGGTGGGAGTATGGGTGTGTAGTTCGTGTGGTGGAGACGCGGCATTTAGTGAGAATCACGGAGGAGAGAGATTTGTGACGGTGGTGGTGGCCGTGAAAAAGAAGAGGGTCTGTTGTGGAAAAGAACAGGGAAAGCTTTCTTTTTCCTCTTTTTCTCTTCCGAAGATAGACTTCATTGAGGTCTATTGGTGTATTTTTTATTTCTTTTTCCTTCAGT
TARGET=487,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4912
PRIMER_LEFT_PENALTY=0.670094
PRIMER_RIGHT_PENALTY=0.821141
PRIMER_LEFT_SEQUENCE=GCCGTGAAAGCAAACTCTACAT
PRIMER_RIGHT_SEQUENCE=CCTGAGTTTCGCTGTCTTGTTT
PRIMER_LEFT=263,22
PRIMER_RIGHT=556,22
PRIMER_LEFT_TM=60.670
PRIMER_RIGHT_TM=60.821
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=1.5097
PRIMER_LEFT_1_PENALTY=1.256192
PRIMER_RIGHT_1_PENALTY=0.253482
PRIMER_LEFT_1_SEQUENCE=AACTCGCCTATCGATCATGTTA
PRIMER_RIGHT_1_SEQUENCE=TTCTTGTTTCTACATCGTCGGA
PRIMER_LEFT_1=374,22
PRIMER_RIGHT_1=652,22
PRIMER_LEFT_1_TM=58.744
PRIMER_RIGHT_1_TM=59.747
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=1.5660
PRIMER_LEFT_2_PENALTY=1.256192
PRIMER_RIGHT_2_PENALTY=0.309858
PRIMER_LEFT_2_SEQUENCE=TAAACTCGCCTATCGATCATGT
PRIMER_RIGHT_2_SEQUENCE=ATCCTGAGTTTCGCTGTCTTGT
PRIMER_LEFT_2=372,22
PRIMER_RIGHT_2=558,22
PRIMER_LEFT_2_TM=58.744
PRIMER_RIGHT_2_TM=60.310
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=1.5660
PRIMER_LEFT_3_PENALTY=1.256192
PRIMER_RIGHT_3_PENALTY=0.309858
PRIMER_LEFT_3_SEQUENCE=AACTCGCCTATCGATCATGTTA
PRIMER_RIGHT_3_SEQUENCE=ATCCTGAGTTTCGCTGTCTTGT
PRIMER_LEFT_3=374,22
PRIMER_RIGHT_3=558,22
PRIMER_LEFT_3_TM=58.744
PRIMER_RIGHT_3_TM=60.310
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=1.8227
PRIMER_LEFT_4_PENALTY=1.256192
PRIMER_RIGHT_4_PENALTY=0.566538
PRIMER_LEFT_4_SEQUENCE=TAAACTCGCCTATCGATCATGT
PRIMER_RIGHT_4_SEQUENCE=TCTACATCGTCGGATTCCATAA
PRIMER_LEFT_4=372,22
PRIMER_RIGHT_4=644,22
PRIMER_LEFT_4_TM=58.744
PRIMER_RIGHT_4_TM=59.433
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_03683 Ps_contig_mira-and-tgicl-ass_franssen_30722
SEQUENCE=AACTNTAGTAANACCATCATCACAATACATAAGCACACAATACTATATTCACAATCAATACAAACTAAATAGCAAACTAATTTCAAATAGCAAAAATTATTTACATTTCTTTAACATATTAAAACTCAAGAATAAAATAATACATTAGAACTCAAACAATATAATTAAAACTACAGAAACCATGCAATAAAAAAAACTTAATAAAACATACATTAAGACTTAAAAGCCAAACATTAAAACTTATATTTAGAATTATACATTTAGCCGTGAAAGCAAACTCTACATCAGAAAGAACCACACCTATTAGAAGCTCTATTTAATCAGTCATCTCAAACTGGTATTTGAACAAAATACATATAATACTTTAAACATTAAACTCGCCTATCGATCATGTTATTAATACGAAAACTAGCTTTAACCTCAAGATCTCAACACAGTAAAATATGTATTCAAATTAAATACATTATAAACAATCAAACTCAATGCCGAATCGAATCGAATCAAAAATTTGAATTATAACACGAATTAATTTTGTAAACAAGACAGCGAAACTCAGGATCAAAATATTACCCGATGAAATGTGTACACGGCTGCTTCACGTCATTGTTGCATAATCAAAAACGTTATGGAATCCGACGATGTAGAAACAAGAACAAAATCAGATTCAAATCTCGGATCAAACTTACAACTTGCACATAAGTTAATGAAAGACAACAACCAAACCGAATATAAAAATGAGTTTCACGAATTTGAAATAGTCGGAAAAAAATAGTTTAGTTAAAGACGTGAGTGTAGCTATTCTTGTGTCCTGAAATCAGAATCACGACGTGTTGGAAGAGAAGATATCCTCCATAGAACCTTCACAATCCTTCTTTTTCCAATCTCTGTCTCCATTCATCTTTGTGGTTTATGTTCCTTCACAGCTGTCATGTATACAGAGAATGAGTTACTCAGTTGATAGGACGTTAGCAGGATGTAAGTTGAGGGTATCAGATGCGCCGAAACTCTGATTGAATCCAATGCACATAATTCATACTTCAGTTAGAGATTTAAATCATGCTATTGTGGTTGTCGTTTTTACGAACACCTTGCAGGTGGTGCGGACAAGGTGAATTGGAGGTGGGGTACGATGGTGTTTCAAGCTGAAGGTTGTGATGAGGGATTTGTTACGGCGTAAGGCGGCAGAGAGCAGTAGAGGTACGACGAGGAGGAGGAGGGAAGTGACTCGCGGCGGAGAAGTTTGGATGGACGGATTAGCATGGCAACGATTGATGTTCGTCGTGTAGGTCGATGGTGTGAAGGCGGTGGGAGTATGGGTGTGTAGTTCGTGTGGTGGAGACGCGGCATTTAGTGAGAATCACGGAGGAGAGAGATTTGTGACGGTGGTGGTGGCCGTGAAAAAGAAGAGGGTCTGTTGTGGAAAAGAACAGGGAAAGCTTTCTTTTTCCTCTTTTTCTCTTCCGAAGATAGACTTCATTGAGGTCTATTGGTGTATTTTTTATTTCTTTTTCCTTCAGT
TARGET=1205,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1058
PRIMER_LEFT_PENALTY=0.067678
PRIMER_RIGHT_PENALTY=0.038090
PRIMER_LEFT_SEQUENCE=TGTCGTTTTTACGAACACCTTG
PRIMER_RIGHT_SEQUENCE=ACTACACACCCATACTCCCACC
PRIMER_LEFT=1069,22
PRIMER_RIGHT=1324,22
PRIMER_LEFT_TM=60.068
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=256
PRIMER_PAIR_PENALTY_1=0.1120
PRIMER_LEFT_1_PENALTY=0.073900
PRIMER_RIGHT_1_PENALTY=0.038090
PRIMER_LEFT_1_SEQUENCE=CATGCTATTGTGGTTGTCGTTT
PRIMER_RIGHT_1_SEQUENCE=ACTACACACCCATACTCCCACC
PRIMER_LEFT_1=1055,22
PRIMER_RIGHT_1=1324,22
PRIMER_LEFT_1_TM=59.926
PRIMER_RIGHT_1_TM=60.038
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.1270
PRIMER_LEFT_2_PENALTY=0.067678
PRIMER_RIGHT_2_PENALTY=0.059316
PRIMER_LEFT_2_SEQUENCE=TGTCGTTTTTACGAACACCTTG
PRIMER_RIGHT_2_SEQUENCE=CTACACGACGAACATCAATCGT
PRIMER_LEFT_2=1069,22
PRIMER_RIGHT_2=1285,22
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=60.059
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=217
PRIMER_PAIR_PENALTY_3=0.1490
PRIMER_LEFT_3_PENALTY=0.110942
PRIMER_RIGHT_3_PENALTY=0.038090
PRIMER_LEFT_3_SEQUENCE=AATCATGCTATTGTGGTTGTCG
PRIMER_RIGHT_3_SEQUENCE=ACTACACACCCATACTCCCACC
PRIMER_LEFT_3=1052,22
PRIMER_RIGHT_3=1324,22
PRIMER_LEFT_3_TM=59.889
PRIMER_RIGHT_3_TM=60.038
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.1772
PRIMER_LEFT_4_PENALTY=0.139129
PRIMER_RIGHT_4_PENALTY=0.038090
PRIMER_LEFT_4_SEQUENCE=TTGTGATGAGGGATTTGTTACG
PRIMER_RIGHT_4_SEQUENCE=ACTACACACCCATACTCCCACC
PRIMER_LEFT_4=1149,22
PRIMER_RIGHT_4=1324,22
PRIMER_LEFT_4_TM=59.861
PRIMER_RIGHT_4_TM=60.038
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=176
=
PRIMER_SEQUENCE_ID=SSR_03684 Ps_contig_mira-and-tgicl-ass_franssen_30723
SEQUENCE=ACACAAGAATAGCTACACTCACGTCTTCAACCCAACTATTTTTTTCTGACTATTTCAAATTCGTGAAACTCATTTTATATTCGGTTTGGTTGCTGTCTGTCATTAATTTATGTGCAGGTTGTAAGTTTGATCCGAGATTTGAATCTGATTTTGTTCTTGTTTCTACATCGTCGGATTCCATAACGTTTTTGATTATGCAACAATGACGTGAAGCAGCCGTGTACACATTTCATCGGGTAATATTTTGATCCTGAGTTTCGCTGTCTTGTTTACAAAATTAATTCGTGTTATAATTCAAATTTTTGATTCGATTCGATTCGGCATTGAGTTTGATTGTTTATA
TARGET=305,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03685 Ps_contig_mira-and-tgicl-ass_franssen_30725
SEQUENCE=CGGGGTNAACAAATCCGAACTCAACGAACTCAACCAAAACTCCGCGAATCCAAAGGCAAAAAAACACACTCTTGTCTGAAACTAACTTCAATTCAATTCAAACATGTTATGCGGCGTCGTTAGCACTTCCTTCACTTCCATCTTACCCAGAAATAAACAACCCTATCTATCCAAATCAATTTCATGCTCCCTCCCAGGAGCTGTGGTTGAAACTAAAGCAACTAATGTTGCTGAACCGCTTTTGCTTAATGCCGTTCGAGGACTAGAGGTTGAAAGACCCCCGGTTTGGCTTATGAGACAAGCTGGACGGTACATGAAGAGTTACCAAGCTATATGTGAGAAACATCCTTCATTTCGCGAAAGGTCTGAAAATGTTGATCTCGTGGTGGAAATTTCGTTGCAACCATGGAAGGTTTTTAAGCCGGATGGGGTAATTTTGTTTTCGGACATTCTTACCCCGCTTTCTGGGATGAATATACCGTTCGATATTGTGAAAGGGAAGGGTCCTGTCATCTTTGATCCTGTTTACTCGGCCGGCCAAGTTGATCAAGTGAGGGAGTTTATTCCGGAAGAATCAGTTCCATATGTCGGTGAAGCATTATCAATTTTGAGGAAAGAGGTGGATAATAAAGCTGCAGTCCTTGGTTTTGTGGGAGCTCCATTCACCTTGGCATCATACGTGGTTGAAGGCGGTTCATCCAAACACTTCTCTAAAATAAAGAGACTGGCTTTCTCTGAACCCAAGATTCTTCACTCATTACTGCAGAAATTCACAACATCAATGGCTCGTTACATCCAATACCAAGCTGACAATGGAGCTCAAGCTGTTCAGATCTTTGATTCATGGGCAACAGAGCTTAGTCCAGTAGATTTCGAACAGTTCAGTTTGCCTTACTTGAAGCAGATCATTGATACCGTGAAGAAAAGCCATCCAGAACTCCCACTTATCCTCTATGCGAGTGGATCGGGTGGCTTGCTCGAGAGAATAGCCGCGACCGGTGTGGATGTAGTTAGCTTGGATTGGACGGTTGATATGGCCGATGGTAGAAAGCGTTTGGGACCAAATGTAGCTATCCAAGGTAACGTTGATCCCGGTGTTCTTTTTGGTTCGAAAGAGCTAATCAGTGATAGGATTTATGATACTGTGAAAAAAGCTGGTAGAGGCAAACACATTTTGAATCTAGGCCATGGTATTGTAGTAGGTACACCTGAGGAGAATGTAGCACATTTTTTTGAGGTTGCAAAAGGAGTCAGATACTAAGTTAATTATGCATTTTTTTATATTAGCATTTTTGTTAATTTAATTTAAGTTAATATAAGAATTTGGCTTGTCAATAATTTTTCTTTACTGTGATTGCGATAAAAATATATTTTTGTAATAAACATTTGTTTTATTTCCTCTTCACTATATTCGAGGAGAATGATCACAACCAATAATGACAAAAACTGCTTGTCTTYC
TARGET=87,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03686 Ps_contig_mira-and-tgicl-ass_franssen_30727
SEQUENCE=TTGATTCACAACATGAACTTGAATTAACATGACATGAAGAATGCAGTCCATAGCAAAGCAGCGTGTTACTGTTTACCAACCAAGACTAGAAGAAGACGTGCTTCGACATTTGAAGAGTAAACATGACATGACATGACAAGTATCAATACCAAGAAACTGGCAAAACATAGACACAAAGGAGTAAAATCATATCTTTAACAACACCTTCCGTGTCCAATATTTCCTATACAAATCCTGTTAGACATATGACAACCTAAGCAGAACATAAAAAACAAGCAGTTCCAATAAAACCTTTTATGGATATCCTTACCCATAAAAAGATTCACAGCTGAAGTAGAGGTAATGCCATAACAACCCCAAAGATGGAGAAGAGTAATAACATCATCCATGTTGGAAATGCCTGTTTTCTGTTTCTCCTCATGTCCTTCAATCCCTCTACCTTTTTCATGTCTTCTACCGCGGAGTCTAAATTCCTTTCCGAAACAGAATTTGAACTTTTATCATGACGAGAATCTGATGATACCCGCTGCTCAGAAGCAGTTTGCTCCGAGAGCTGCTGCTGTTCGTACTTCTCCACGTACTCGGGGAACATTTTCCTGAATGTTGCATTCTTGCAATTGAATGAGAGAGAAGATTTTGCAAGACGCTGCTTCTCGGCAATAGTGGTGTTTACACTGCCAGTGGTTGGACTGGTGTCCATCATAAATGAAAGAAGCCCGGTAAGTATGCTTGATACAGACCACATTGGATTCCAACTTTCAGGATGAAAATCACTCATTGATAAACAGATCTTCTTCTGTGTCATGAATCGCCCATTGGGTGTTGTCATGCTGATTCCAGGAGGTTTATATGGATATTCTGGCGGAAACTTGATTTTTCCATAGTAAAAACCACCTGAGAAAGGTGTTCCCTCGCTTCCTTCCAATACGTAGTGCCACTCAAGAATGTCACTTGGTGAAGGACGGGCCACAATATGGGAAACTGGCTCTTTACAAAGGGCTCTATACTCCTTCTGAAGACGCTTGATGCATGCTTTATCCGCCATTCAACTAATTCAGAGCAGTATAGACAAGGACTGCGCTATATCCTGTTTCTTTATTCTCAGAAAAATTTCCTTCTGAGATCCGATTTCGCGAAGAAAAAATTGGTGGCAAAATCGATTATTTCCGCGTGAACAAAATTAGCCCCTTTTTGGAAACAGAAACAGCGAAAAGAAGTTCCAAAACGGCGGCGTTGAATATAGAGAAATGAAAGAGAGAGTAGAGTGAGTTTAGAACTGCGTTCCCCGCGTACTCTGCGTTGA
TARGET=121,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0698
PRIMER_LEFT_PENALTY=0.037486
PRIMER_RIGHT_PENALTY=0.032347
PRIMER_LEFT_SEQUENCE=GCGTGTTACTGTTTACCAACCA
PRIMER_RIGHT_SEQUENCE=CCTTTGTGTCTATGTTTTGCCA
PRIMER_LEFT=60,22
PRIMER_RIGHT=178,22
PRIMER_LEFT_TM=59.963
PRIMER_RIGHT_TM=60.032
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.1607
PRIMER_LEFT_1_PENALTY=0.128329
PRIMER_RIGHT_1_PENALTY=0.032347
PRIMER_LEFT_1_SEQUENCE=AGCAGCGTGTTACTGTTTACCA
PRIMER_RIGHT_1_SEQUENCE=CCTTTGTGTCTATGTTTTGCCA
PRIMER_LEFT_1=56,22
PRIMER_RIGHT_1=178,22
PRIMER_LEFT_1_TM=59.872
PRIMER_RIGHT_1_TM=60.032
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=123
PRIMER_PAIR_PENALTY_2=0.1695
PRIMER_LEFT_2_PENALTY=0.137183
PRIMER_RIGHT_2_PENALTY=0.032347
PRIMER_LEFT_2_SEQUENCE=TAGCAAAGCAGCGTGTTACTGT
PRIMER_RIGHT_2_SEQUENCE=CCTTTGTGTCTATGTTTTGCCA
PRIMER_LEFT_2=50,22
PRIMER_RIGHT_2=178,22
PRIMER_LEFT_2_TM=60.137
PRIMER_RIGHT_2_TM=60.032
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=129
PRIMER_PAIR_PENALTY_3=0.2599
PRIMER_LEFT_3_PENALTY=0.227550
PRIMER_RIGHT_3_PENALTY=0.032347
PRIMER_LEFT_3_SEQUENCE=GCAGCGTGTTACTGTTTACCAA
PRIMER_RIGHT_3_SEQUENCE=CCTTTGTGTCTATGTTTTGCCA
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=178,22
PRIMER_LEFT_3_TM=60.228
PRIMER_RIGHT_3_TM=60.032
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=0.2965
PRIMER_LEFT_4_PENALTY=0.264143
PRIMER_RIGHT_4_PENALTY=0.032347
PRIMER_LEFT_4_SEQUENCE=CATGAAGAATGCAGTCCATAGC
PRIMER_RIGHT_4_SEQUENCE=CCTTTGTGTCTATGTTTTGCCA
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=59.736
PRIMER_RIGHT_4_TM=60.032
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_03687 Ps_contig_mira-and-tgicl-ass_franssen_30737
SEQUENCE=GGTTCGGCCCAGGGTCTTTTCCTAGGGAAATACTTTTTCCTTGCAGACTGCTTCCTGCAGGGCCGGGAGAAGATGGCAGAGGAGTATAAGGTCTAGCAAAAGCTGATGTCGATGACGACAACTGGGTCACTCCATATAGAGAAGTAGCCCCGACAGCAGGTTGCGCACCAGGAGATAACACGGGACTTACAGGAGCCGAATAAGGACTCCATCCAGGAAAAGGAACAACCCCTGGGGAAAGAAGCACAGGACCATAAGCTCCCTGTACATATGAACCGGGTAACACAGGAGGTCTAGCCACTCTCAAGCTTGTGGAAGCTCCTCCAAACTGCTCGGGCAGAGGAACAGTTGGAGATTGCACCTGTTGATAAAATTGAGGTGCAGATGCTGGCAATGACGTGCCTGCAGGTTGAGGATGGTGGAATTTACATGTTACGCCAAATTTGCACTGCCCCGTTTTCAAATAATAGGAGCACTCATTCTCACCCGGACGTAATGGGTATCCATAAATATTTAGTGGAGCTTGGCTTAAATATCCCCCTCCATTTTTGGGATGGTGGAATTTACAGGACGCGCCAAATTTACAGGTTCCAGTTTTTAAATAATACTGACACGGAGGTTCCCCAAGCCGTTCAGGGTAGTCCCCTGTAGCTCTTACGGCAGCAGCAACCGCAGCTCGATCACGGGGGTGATTGAAACGACACCTGCCACCATAACCACAAAACCCAGTTCTCATATAATAAGCACAATTAGGCACACCATGTCTCTCCGGGTAAGATTCACCACCACCACCACCACCAAGCGTCAAATGCCACATCGACTCTCCGAGAGCAGCTTCAGCTGTCGGTGAACGCCATTCATGTTGTTGACTAAGCGGGTTGGATCCATTTCTAGTGGGGCTCCGGCCGTACAAGTCCATTCGTGTGAGGCTCCTGTACAGAAACTTTACACTTTGATCTACAAATGTTCCGGAAGATCGGCGAGCTTAGCTCGTCTTACTTCCTCGTGAGACAAAAATGGGTTTTTGTTCTCACACGTTCCTTGTGTTGTTCGTTCGTCTGTNTGTGTTTGTGTCTTTCTAGTTTTTCACTCTCTCTCTGGAACTGTNCNNNTACTGA
TARGET=781,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1490
PRIMER_LEFT_PENALTY=0.038361
PRIMER_RIGHT_PENALTY=0.110660
PRIMER_LEFT_SEQUENCE=CATGTCTCTCCGGGTAAGATTC
PRIMER_RIGHT_SEQUENCE=AACAACACAAGGAACGTGTGAG
PRIMER_LEFT=759,22
PRIMER_RIGHT=1050,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=60.111
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=0.2204
PRIMER_LEFT_1_PENALTY=0.038361
PRIMER_RIGHT_1_PENALTY=0.182028
PRIMER_LEFT_1_SEQUENCE=CATGTCTCTCCGGGTAAGATTC
PRIMER_RIGHT_1_SEQUENCE=CAAGGAACGTGTGAGAACAAAA
PRIMER_LEFT_1=759,22
PRIMER_RIGHT_1=1043,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=60.182
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.2617
PRIMER_LEFT_2_PENALTY=0.079714
PRIMER_RIGHT_2_PENALTY=0.182028
PRIMER_LEFT_2_SEQUENCE=CACAATTAGGCACACCATGTCT
PRIMER_RIGHT_2_SEQUENCE=CAAGGAACGTGTGAGAACAAAA
PRIMER_LEFT_2=744,22
PRIMER_RIGHT_2=1043,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=60.182
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.3590
PRIMER_LEFT_3_PENALTY=0.038361
PRIMER_RIGHT_3_PENALTY=0.320679
PRIMER_LEFT_3_SEQUENCE=CATGTCTCTCCGGGTAAGATTC
PRIMER_RIGHT_3_SEQUENCE=ACAAGGAACGTGTGAGAACAAA
PRIMER_LEFT_3=759,22
PRIMER_RIGHT_3=1044,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=59.679
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=0.5001
PRIMER_LEFT_4_PENALTY=0.038361
PRIMER_RIGHT_4_PENALTY=0.461740
PRIMER_LEFT_4_SEQUENCE=CATGTCTCTCCGGGTAAGATTC
PRIMER_RIGHT_4_SEQUENCE=TGTCTCACGAGGAAGTAAGACG
PRIMER_LEFT_4=759,22
PRIMER_RIGHT_4=1012,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=59.538
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_03688 Ps_contig_mira-and-tgicl-ass_franssen_30737
SEQUENCE=GGTTCGGCCCAGGGTCTTTTCCTAGGGAAATACTTTTTCCTTGCAGACTGCTTCCTGCAGGGCCGGGAGAAGATGGCAGAGGAGTATAAGGTCTAGCAAAAGCTGATGTCGATGACGACAACTGGGTCACTCCATATAGAGAAGTAGCCCCGACAGCAGGTTGCGCACCAGGAGATAACACGGGACTTACAGGAGCCGAATAAGGACTCCATCCAGGAAAAGGAACAACCCCTGGGGAAAGAAGCACAGGACCATAAGCTCCCTGTACATATGAACCGGGTAACACAGGAGGTCTAGCCACTCTCAAGCTTGTGGAAGCTCCTCCAAACTGCTCGGGCAGAGGAACAGTTGGAGATTGCACCTGTTGATAAAATTGAGGTGCAGATGCTGGCAATGACGTGCCTGCAGGTTGAGGATGGTGGAATTTACATGTTACGCCAAATTTGCACTGCCCCGTTTTCAAATAATAGGAGCACTCATTCTCACCCGGACGTAATGGGTATCCATAAATATTTAGTGGAGCTTGGCTTAAATATCCCCCTCCATTTTTGGGATGGTGGAATTTACAGGACGCGCCAAATTTACAGGTTCCAGTTTTTAAATAATACTGACACGGAGGTTCCCCAAGCCGTTCAGGGTAGTCCCCTGTAGCTCTTACGGCAGCAGCAACCGCAGCTCGATCACGGGGGTGATTGAAACGACACCTGCCACCATAACCACAAAACCCAGTTCTCATATAATAAGCACAATTAGGCACACCATGTCTCTCCGGGTAAGATTCACCACCACCACCACCACCAAGCGTCAAATGCCACATCGACTCTCCGAGAGCAGCTTCAGCTGTCGGTGAACGCCATTCATGTTGTTGACTAAGCGGGTTGGATCCATTTCTAGTGGGGCTCCGGCCGTACAAGTCCATTCGTGTGAGGCTCCTGTACAGAAACTTTACACTTTGATCTACAAATGTTCCGGAAGATCGGCGAGCTTAGCTCGTCTTACTTCCTCGTGAGACAAAAATGGGTTTTTGTTCTCACACGTTCCTTGTGTTGTTCGTTCGTCTGTNTGTGTTTGTGTCTTTCTAGTTTTTCACTCTCTCTCTGGAACTGTNCNNNTACTGA
TARGET=1090,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03689 Ps_contig_mira-and-tgicl-ass_franssen_30739
SEQUENCE=ACAGTGGTAGTCAACCGCANAGTANNCGGGGAAACACAACTCCATGCAGAAACTTTTCTTCCCTACACACCATAATTCATTTCATACATATCATAAACATATCATTTACTATCATATAGTATAGAAACTATATGCTAAAATTAATTTCCATATTTCCACAACAACCACCGCAATGAGTAGCATTAAGTTTCTCCAAGCTATAACCGTTACAGTACTGATATTAACATTATCACTCACCGCCACCGTCCAGTCATGTCCACCGTCAGACAGGGCGGCGCTACTAGCCTTCAGAGCAGCTCTCCATGATCCCTACCTCGGCATCTTCAACTCATGGACCGGCACCGACTGTTGCCACAAATGGTACGGTGTCAGCTGCGACAGAGGCTCCCGCCGCGTCGCCGATATCAACCTCCGCGGCGAGTCAGAAGATCCAATCTTCGAAAAAGCACACCGCACCGGCTTCATGACCGGTTACATCTCACCAGCAATCTGCAAACTCAACCGCCTCTCAAGCATAACCGTCGCCGACTGGAAAAACATCTCCGGCGAAATCCCCCGCTGTATAACCTCCCTTCCTTTCCTCCGCATCATCGACCTAATCGGAAACCGTATCTCCGGTCCTATCCCCTATAACATCGGCAAACTCCAGCGTCTCACCGTTTTAAACATCGCCGACAACTCCATCTCCGGTTACCTCCCACCGTCGCTAACCAACCTCAGAAGCCTAATGCACCTCGACATCCGTAACAACCAGATCTCCGGGCCCATACCTAACGACATCGGCAGGCTTCCAATGCTGAGCCGGGCTTTACTTAGCGGTAACAAAATCAGCGGGCCCATACCCGAATCAATTTCTCGTATTTACCGTCTCGCAGATCTAGATCTATCTCGTAACCAAGTTTCCGGGCCCATACCCGAATCACTAGGTAAAATGGCGGTTTTATCCACGCTTAATTTAGATATGAATCATATCTCTGGTCCTATTCCAATAACTCTTTTTAACTCAGGAATAAGTGATTTAAATTTAAGCCGAAACAGTTTGGAAGGTAATATACCTGATGTGTTTGGTGTGAGATCTTATTTCACGGTTTTGGATTTGTCTTATAATAGACTTAAAGGGAGTATTCCAAAATCTATGGCTTCTGCTTCTTATATTGGACATTTGGATTTGAGTTATAATCATCTTTGTGGTAAGATTCCTGATGGAGTTCCTTTTGATCATTTGGAGGCTTCATCTTTTGTGTACAATGATTGTCTTTGTGGGAAGCCACTTAAAGCTTGTGTTAATTAAACATACTTAGTTTGTTATTTCATCAAGTTAATTATATGTAATTTGTTATAGGAACAAGAGTCTTGTGTTTGTTTGTTACTAGTAGTTGTTAGTAGATTCCCATGTATTTGTGTTTGTAAGTTTAAGCATCATATGATATATTGCATTACATTATAGTATTATTACAATATAAATATGAATGGGCCAATGCTATATAATTAAGATACGATGTTAATTGTTTGTTCCCTGATTTTGTAACAAGAAATAGGCAACATTGTAAGCTCTTCTACTGATGGGATAATAAAATCCAGCTTTAC
TARGET=1357,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.4335
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=2.373576
PRIMER_LEFT_SEQUENCE=TCCTGATGGAGTTCCTTTTGAT
PRIMER_RIGHT_SEQUENCE=GCATTGGCCCATTCATATTT
PRIMER_LEFT=1197,22
PRIMER_RIGHT=1480,20
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=59.626
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=2.5482
PRIMER_LEFT_1_PENALTY=0.174657
PRIMER_RIGHT_1_PENALTY=2.373576
PRIMER_LEFT_1_SEQUENCE=GGAAGCCACTTAAAGCTTGTGT
PRIMER_RIGHT_1_SEQUENCE=GCATTGGCCCATTCATATTT
PRIMER_LEFT_1=1262,22
PRIMER_RIGHT_1=1480,20
PRIMER_LEFT_1_TM=59.825
PRIMER_RIGHT_1_TM=59.626
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=2.5594
PRIMER_LEFT_2_PENALTY=0.185822
PRIMER_RIGHT_2_PENALTY=2.373576
PRIMER_LEFT_2_SEQUENCE=GTTCCTTTTGATCATTTGGAGG
PRIMER_RIGHT_2_SEQUENCE=GCATTGGCCCATTCATATTT
PRIMER_LEFT_2=1207,22
PRIMER_RIGHT_2=1480,20
PRIMER_LEFT_2_TM=59.814
PRIMER_RIGHT_2_TM=59.626
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=2.6271
PRIMER_LEFT_3_PENALTY=0.253482
PRIMER_RIGHT_3_PENALTY=2.373576
PRIMER_LEFT_3_SEQUENCE=TGGAGGCTTCATCTTTTGTGTA
PRIMER_RIGHT_3_SEQUENCE=GCATTGGCCCATTCATATTT
PRIMER_LEFT_3=1223,22
PRIMER_RIGHT_3=1480,20
PRIMER_LEFT_3_TM=59.747
PRIMER_RIGHT_3_TM=59.626
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=2.7339
PRIMER_LEFT_4_PENALTY=0.360320
PRIMER_RIGHT_4_PENALTY=2.373576
PRIMER_LEFT_4_SEQUENCE=AATGATTGTCTTTGTGGGAAGC
PRIMER_RIGHT_4_SEQUENCE=GCATTGGCCCATTCATATTT
PRIMER_LEFT_4=1246,22
PRIMER_RIGHT_4=1480,20
PRIMER_LEFT_4_TM=60.360
PRIMER_RIGHT_4_TM=59.626
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_03690 Ps_contig_mira-and-tgicl-ass_franssen_30740
SEQUENCE=AACCTGTAATCTGTATTGCATAGTTAGAGCTTCCATATGAGCTTAACCAAATTAATTTCAATTCAATGTGACAATGAATGACCCGAACAACAATACATACAATATATATAATATAGAGAAGATGATCCTAACCCAGAGACTTTTTCTGTAAACATTTCCAATAATTTAGACTCTAAAACTCTTCCATACTAATAAAACATATCATAAACTCAGAATTCCGGTACTCTAACAGCATCACGAGGCGTATCGAAAGCATCTTTTGGCCTTGACTTCTTTACACCAGCAGTCACCCAAATCTTATCAGATTTGTAACCTTCAACTGTCACACTTGTCACCTTAACCCATACAAGAACTTTGGTTTTCATTCCTTCTATTGCCGAAAGCTTTCCCCTTGTAAGAACAGCTTTGACACGGGTTGCGTATCGCAGTACAGAGGAATCCTTGAAGCTTACCTCACATGCAGATGGCAAGTAGACAATCAACTTACCCTTGATTTCGTCGAACTCATAACAGATTGTGTTTTGAGGAAATAGCCCCGGCGGTAAATTGAAGTCGCGGAGAAGATCTGGTAAGCTTTTTTGTGGCTTACCTTTGAGTTTGTTAAAAACCCATTTGGCCTTCTCTTCAACTGTATTAGAGAAAGTATTGATGTCTTGAGAGATGTTAGAGAACTCTTCTTTAGCTTTTTTAGTAAACCATGAATCTCCAAGTCCTATCTTCAAGCTTGTTACTTTAGTAAGAGCTTTCTCCATCTTAGGTTGTTTTTGCAATGAGATGAGAGAATCTCTCTATCTCTCTCTCACCCAGATGAAGAAAATGAAGGGAAATGAGAACACAAAGAACACAAGACAAGGAAAAAGGGTA
TARGET=792,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6794
PRIMER_LEFT_PENALTY=0.256503
PRIMER_RIGHT_PENALTY=0.422888
PRIMER_LEFT_SEQUENCE=GTAAATTGAAGTCGCGGAGAAG
PRIMER_RIGHT_SEQUENCE=TCCCTTCATTTTCTTCATCTGG
PRIMER_LEFT=541,22
PRIMER_RIGHT=824,22
PRIMER_LEFT_TM=60.257
PRIMER_RIGHT_TM=60.423
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=1.1146
PRIMER_LEFT_1_PENALTY=0.691680
PRIMER_RIGHT_1_PENALTY=0.422888
PRIMER_LEFT_1_SEQUENCE=TTGTGGCTTACCTTTGAGTTTG
PRIMER_RIGHT_1_SEQUENCE=TCCCTTCATTTTCTTCATCTGG
PRIMER_LEFT_1=578,22
PRIMER_RIGHT_1=824,22
PRIMER_LEFT_1_TM=59.308
PRIMER_RIGHT_1_TM=60.423
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=1.1411
PRIMER_LEFT_2_PENALTY=0.718261
PRIMER_RIGHT_2_PENALTY=0.422888
PRIMER_LEFT_2_SEQUENCE=ATTTGGCCTTCTCTTCAACTGT
PRIMER_RIGHT_2_SEQUENCE=TCCCTTCATTTTCTTCATCTGG
PRIMER_LEFT_2=610,22
PRIMER_RIGHT_2=824,22
PRIMER_LEFT_2_TM=59.282
PRIMER_RIGHT_2_TM=60.423
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=1.1534
PRIMER_LEFT_3_PENALTY=0.691680
PRIMER_RIGHT_3_PENALTY=0.461713
PRIMER_LEFT_3_SEQUENCE=TTGTGGCTTACCTTTGAGTTTG
PRIMER_RIGHT_3_SEQUENCE=CCCTTTTTCCTTGTCTTGTGTT
PRIMER_LEFT_3=578,22
PRIMER_RIGHT_3=861,22
PRIMER_LEFT_3_TM=59.308
PRIMER_RIGHT_3_TM=59.538
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=1.1800
PRIMER_LEFT_4_PENALTY=0.718261
PRIMER_RIGHT_4_PENALTY=0.461713
PRIMER_LEFT_4_SEQUENCE=ATTTGGCCTTCTCTTCAACTGT
PRIMER_RIGHT_4_SEQUENCE=CCCTTTTTCCTTGTCTTGTGTT
PRIMER_LEFT_4=610,22
PRIMER_RIGHT_4=861,22
PRIMER_LEFT_4_TM=59.282
PRIMER_RIGHT_4_TM=59.538
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_03691 Ps_contig_mira-and-tgicl-ass_franssen_30744
SEQUENCE=AAACCCTAAACCTAACCCTTCATTTGGAGAGAGAGAGTGAGAGACAGAGAGAGAGGAGAAGATGAAGTTCAATCCAAGGGTTTCCAGCAGCCGCCGCAAGAGCCGTAAAGCTCATTTCACAGCACCGTCAAGCGTCCGCCGCGTGCTAATGAGCGCTCCACTCTCCGGCGACCTTCGATCCAAATACAACGTCCGTTCCTTACCGGTTCGCAAGGACGACGAGGTTCAGGTAGTTAGAGGAACATTCAAAGGACGTGAAGGAAAGATCGTTCAGGTTTATCGCAAAAAGTGGGTGATTCACATCGAGCGCATAACTCGCGAGAAAGTTAACGGTTCAACGGTTAACGTTGGGTTGGACCCTTCCAAGGTGGTTATCACGAAGCTCCGTTTGGATAAGGATCGGAAGTCGTTGATTGATAGGAAGGCGAAGGGTCGTGCTGCTGCTGATAAGGAAAAGGGTACTAAGTTTGCTCCTGAAGATATCATGCAAACCATTGATTAGGGTTTTGTTTTTCTATTGTTGTTATTGTTATAACTAGTTTTGATTTTTGTTATTGGATCTGAATGAAACAATTGAGTATTTTGTGGCCTTTAAATTTTGGTTTTGATTTAGTGAATGAAATTGCATGTTTACATTCTCAA
TARGET=27,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2171
PRIMER_LEFT_PENALTY=1.107582
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=CCTAAACCTAACCCTTCATTTGG
PRIMER_RIGHT_SEQUENCE=GATCTTTCCTTCACGTCCTTTG
PRIMER_LEFT=4,23
PRIMER_RIGHT=267,22
PRIMER_LEFT_TM=60.108
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=1.2171
PRIMER_LEFT_1_PENALTY=1.107582
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=CCTAAACCTAACCCTTCATTTGG
PRIMER_RIGHT_1_SEQUENCE=ATCTTTCCTTCACGTCCTTTGA
PRIMER_LEFT_1=4,23
PRIMER_RIGHT_1=266,22
PRIMER_LEFT_1_TM=60.108
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=1.2171
PRIMER_LEFT_2_PENALTY=1.107582
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=CCCTAAACCTAACCCTTCATTTG
PRIMER_RIGHT_2_SEQUENCE=GATCTTTCCTTCACGTCCTTTG
PRIMER_LEFT_2=3,23
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=60.108
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=1.2171
PRIMER_LEFT_3_PENALTY=1.107582
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=CCCTAAACCTAACCCTTCATTTG
PRIMER_RIGHT_3_SEQUENCE=ATCTTTCCTTCACGTCCTTTGA
PRIMER_LEFT_3=3,23
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=60.108
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=1.3396
PRIMER_LEFT_4_PENALTY=1.107582
PRIMER_RIGHT_4_PENALTY=0.232060
PRIMER_LEFT_4_SEQUENCE=CCTAAACCTAACCCTTCATTTGG
PRIMER_RIGHT_4_SEQUENCE=ACGTTGTATTTGGATCGAAGGT
PRIMER_LEFT_4=4,23
PRIMER_RIGHT_4=191,22
PRIMER_LEFT_4_TM=60.108
PRIMER_RIGHT_4_TM=59.768
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_03692 Ps_contig_mira-and-tgicl-ass_franssen_30744
SEQUENCE=AAACCCTAAACCTAACCCTTCATTTGGAGAGAGAGAGTGAGAGACAGAGAGAGAGGAGAAGATGAAGTTCAATCCAAGGGTTTCCAGCAGCCGCCGCAAGAGCCGTAAAGCTCATTTCACAGCACCGTCAAGCGTCCGCCGCGTGCTAATGAGCGCTCCACTCTCCGGCGACCTTCGATCCAAATACAACGTCCGTTCCTTACCGGTTCGCAAGGACGACGAGGTTCAGGTAGTTAGAGGAACATTCAAAGGACGTGAAGGAAAGATCGTTCAGGTTTATCGCAAAAAGTGGGTGATTCACATCGAGCGCATAACTCGCGAGAAAGTTAACGGTTCAACGGTTAACGTTGGGTTGGACCCTTCCAAGGTGGTTATCACGAAGCTCCGTTTGGATAAGGATCGGAAGTCGTTGATTGATAGGAAGGCGAAGGGTCGTGCTGCTGCTGATAAGGAAAAGGGTACTAAGTTTGCTCCTGAAGATATCATGCAAACCATTGATTAGGGTTTTGTTTTTCTATTGTTGTTATTGTTATAACTAGTTTTGATTTTTGTTATTGGATCTGAATGAAACAATTGAGTATTTTGTGGCCTTTAAATTTTGGTTTTGATTTAGTGAATGAAATTGCATGTTTACATTCTCAA
TARGET=46,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5214
PRIMER_LEFT_PENALTY=0.411917
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=CCTAACCCTTCATTTGGAGAGA
PRIMER_RIGHT_SEQUENCE=GATCTTTCCTTCACGTCCTTTG
PRIMER_LEFT=10,22
PRIMER_RIGHT=267,22
PRIMER_LEFT_TM=59.588
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.5214
PRIMER_LEFT_1_PENALTY=0.411917
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=CCTAACCCTTCATTTGGAGAGA
PRIMER_RIGHT_1_SEQUENCE=ATCTTTCCTTCACGTCCTTTGA
PRIMER_LEFT_1=10,22
PRIMER_RIGHT_1=266,22
PRIMER_LEFT_1_TM=59.588
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.5959
PRIMER_LEFT_2_PENALTY=0.543494
PRIMER_RIGHT_2_PENALTY=0.052366
PRIMER_LEFT_2_SEQUENCE=CCCTTCATTTGGAGAGAGAGAG
PRIMER_RIGHT_2_SEQUENCE=GTGCTGTGAAATGAGCTTTACG
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=124,22
PRIMER_LEFT_2_TM=59.457
PRIMER_RIGHT_2_TM=59.948
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=110
PRIMER_PAIR_PENALTY_3=0.6440
PRIMER_LEFT_3_PENALTY=0.411917
PRIMER_RIGHT_3_PENALTY=0.232060
PRIMER_LEFT_3_SEQUENCE=CCTAACCCTTCATTTGGAGAGA
PRIMER_RIGHT_3_SEQUENCE=ACGTTGTATTTGGATCGAAGGT
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=191,22
PRIMER_LEFT_3_TM=59.588
PRIMER_RIGHT_3_TM=59.768
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=0.6530
PRIMER_LEFT_4_PENALTY=0.543494
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=CCCTTCATTTGGAGAGAGAGAG
PRIMER_RIGHT_4_SEQUENCE=GATCTTTCCTTCACGTCCTTTG
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=267,22
PRIMER_LEFT_4_TM=59.457
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_03693 Ps_contig_mira-and-tgicl-ass_franssen_30745
SEQUENCE=AGTACGCGGGACAAAAAAAACTTTCTCCATTTCCAAAACTCTCATATTCTGAGCTTTCATCTTTCAACCAAAAAAAAAAGATTRAACATTCGTGTGTTGTTTTTTTCTTCTGATAAGCGAGTTAAGAGAAAATGACTTGCGTAGCAGAAGCTGAGCGTGACAAATACAACATGATAAATTTCGAAGAGACCGAGTTGCGACTCGGTCTTCCCGGTGGCTCAGTGAGTGATCAGGGCGAGCCATCGGTTAAAAGCAGTTGCGGAAAGAGAGGTTTCGCAGAAACTGCTAATATGGATTTGAAGCTTAATCTTTCTCCAAGTAATGATTCTGTTTCATCTTCTTCAACTATATCTTCTGTTACTGCAAACAAAGGCAAAGAGAAAACTTCAACAACAGTTACTGCACCTCCTCGCGCTAAAGCACAAGTTGTGGGATGGCCACCAGTTAGATCCTTCAGAAAGAACATAGTGAATGTTCAAAAGAGCAACAATGAATCAGAACCAGAAAAAAACACAAGTGTTGGAAATGGAGCATTTGTTAAAGTGAGCATGGATGGTGCACCTTATCTACGTAAGGTAGACCTTAACTTGTACAAGAGCTACCAAGAGCTGTCAGATGCGCTGGCTAAAATGTTTAGTTCTTTCACAATTGACAACTGTGGGTCCCAAGTTACCAAAGACTTTATGAATGAAAGCAAACTGATTGACCTCTTGAACGGCTCTGATTATGTACCAACTTATGAAGACAAAGATGCTGATTGGATGCTTGTCGGCGATGTTCCTTGGGAAATGTTTGTTCAATCCTGCAAGCGCCTTCGAATAATGAAAGGCTCTGAGGCAATTGGGCTAGCGCCAAGGGCAGTGGAAAAGTGCAAGAACAGAAGCTGAAAACCTCGTTATCATGTTCCGACAAAAATCCTCTTGTTGTTGATCATGTATTGGCGTTCCAAGGAAGCAAATTGGGCTCGAGTAAGATCGGGGCTAATATAGGAATAATAAGAGTTCAATTATTATTATTATTATTATTATTATGTGTNTGTGTTATTTNTTTGTNTTTTTTCTNTTATATNTNTATTATATA
TARGET=1006,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03694 Ps_contig_mira-and-tgicl-ass_franssen_30747
SEQUENCE=TCGRNGGAGTGGGGAGTGTGCAAAGTTGTCTGTATAGGTTGCATGAAGTGCTCCACTTCAAGAACACCACGTGTATATCACTAGCTTCCACCTTGAAACACGTCCACATTTTTTACCCTCTCTCTCAAAACCGACCTCATAGAAGAGAGAGAGAGATAGAGATATTGATTAATTAAGCAAGCACACACTAGCACAACACTATGGCCGGTGGTAGCAAATTCCTCACTCTTGTTCACTCATCTTCAAGATCTTCTTTTCTCAAACCCTCTACACCATTCTTCTACAACACCATCAAGAATTATGGGCAAGAAATCAAGGATAAACAGAGTCATTTGATAAAAGAGAGGGCACCATCTACAGCTGAAGAGTTCTTGAGAGTGGCAGAGGAAAGAGCAAATGAAACACCAAAAGTGAAAAGTCAAACCGTTGATAAAGCATTTGAAGCTGCTGAGGAAGCCACTAAGAGTAACTCAAACACTCAAAATGTTAAGAACAAGTACAAGGAGCATTAACATTAGGAATTTGCTATGTTCACTTCAGAATCAATTCTGAGATTGTTGTTTTTTTAAGCTAGAATAAGGGATTTCCAAAAAGTTTATTTATTTGCAAGTATTATGGATTTATGAGTTTAACTAATTGTATTGTCTTTTTTGTAAAATAATATGAAATTGTTGGTTTGTACTATGTTTGAACTTAATCTCCTTTTTTAAAAATATTGATGGGGATTTCATGAATGAATCATTAGCCTTTTATTTA
TARGET=144,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03695 Ps_contig_mira-and-tgicl-ass_franssen_30748
SEQUENCE=GTCGGGGAAATTCTTCTCATCTCTCTAACATTCTCTCTCTCTAACATTTATCACAAAACTTTCAGTGTCTCCAATCAGCATCTTCAAACTGGCGTTCGAGAATCAAGACACAGTTCGTGAAATCAAGCCCAAAAATAGGAGAATCATGGGTGCTGGAGGACCTGATGATGAAGAAAACAGATGGCCGCCATGGTTGAAACCTCTGCTGAAAGAGCGTTTCTTTGTTCAATGTAAGATGCACGCTGATTCGCATAAGAGTGAGTGTAATATGTACTGTTTGGATTGTATGAATGGTGCTCTTTGTTCTCTCTGCCTCAATTATCATAAAGATCATCGCGCTATTCAGATAAGGAGGTCTTCATACCATGATGTTATTAGAGTGAATGAGATTCAGAAGGTGTTGGACATATCCGGTGTTCAAACCTATATTATCAACAGTGCTAGGGTCGTGTTCTTGAATGAAAGGCCTCAGCCAAGGCCGGGCAAAGGTGTCACCAACACCTGTGAAGTCTGCGAGCGAAGCCTTCTCGATTCGTTTCGATTCTGTTCTCTTGGTTGCAAGATTGTTGGAACATCAACGAGTTTCCAGAAGAAGAACAAGTTAGCAACCTTGATATCGGACTCGGAGGATTCATACAGCAGCAGTTGCAGCCATGGAAAACTGAAGAACAACATCAAGGTGCAGGTGCAAAGCTTCACTCCTTCAACACCACCACCAACTTCAGTTAATTACAGAACAGCCAAAAGAAGAAAGGGAATCCCACATCGTGCCCCAATGGGAGGACTAGTCTTGCAGTACTAGTCAACAGTTCATAGTTTTAGGTTACTGCTATAATGCCCTTAATGAACCCGGTTCCCCTAACGTTAGAGATTTCCCGCATTCACCTAGTCAGAACATTGACATAATGTTCCGACTATGTGAATGTGGATTCTTTTTGACAGAGGGAACTTTGGTTCAACTTTAATATATAGATACCAAAAAAAATCTAAAGAATCTGTCTTAATGTTGCTCATCAGTTCCAGTGCTAGAGCTTTTTGGTGAAAAGGGCCAGTGCTGGAATTGTTGTTGGTGTTAATAGTGTTTGTTTTTGTGTGTATAGGAAAAATCCAATAGAAGTGGGAAAATGTTGTATAAGCAGAAGAAAAATAAAACTTATGTAAAATACTAGTTTGATAAAAAGGAACGGTGTAGGAAAATAAGCTAAAAGTTCCTTTGGACCAAAGCTAAAGATGGTAATGACAACAGTATAGGTTGGTTTTCTAGAGTAATTATATCCAAGTTTAGGGGGTGTAAATAGTGAAAAAGGTGACAATGACAGTGTGCTCTTTACAAATATAGGTCGACTCTATCTTTATGTAAAATTGTGAAATTCATAATAATGATAATTTATATAATATCTTGTAGGTTAATATAC
TARGET=32,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8545
PRIMER_LEFT_PENALTY=0.792632
PRIMER_RIGHT_PENALTY=0.061818
PRIMER_LEFT_SEQUENCE=GTCGGGGAAATTCTTCTCATCT
PRIMER_RIGHT_SEQUENCE=AACAAAGAAACGCTCTTTCAGC
PRIMER_LEFT=0,22
PRIMER_RIGHT=225,22
PRIMER_LEFT_TM=60.793
PRIMER_RIGHT_TM=60.062
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=226
PRIMER_PAIR_PENALTY_1=0.9518
PRIMER_LEFT_1_PENALTY=0.792632
PRIMER_RIGHT_1_PENALTY=0.159203
PRIMER_LEFT_1_SEQUENCE=GTCGGGGAAATTCTTCTCATCT
PRIMER_RIGHT_1_SEQUENCE=AATCAGCGTGCATCTTACATTG
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=247,22
PRIMER_LEFT_1_TM=60.793
PRIMER_RIGHT_1_TM=60.159
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.9635
PRIMER_LEFT_2_PENALTY=0.792632
PRIMER_RIGHT_2_PENALTY=0.170825
PRIMER_LEFT_2_SEQUENCE=GTCGGGGAAATTCTTCTCATCT
PRIMER_RIGHT_2_SEQUENCE=GAGCACCATTCATACAATCCAA
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=298,22
PRIMER_LEFT_2_TM=60.793
PRIMER_RIGHT_2_TM=59.829
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=0.9714
PRIMER_LEFT_3_PENALTY=0.909595
PRIMER_RIGHT_3_PENALTY=0.061818
PRIMER_LEFT_3_SEQUENCE=CGGGGAAATTCTTCTCATCTCT
PRIMER_RIGHT_3_SEQUENCE=AACAAAGAAACGCTCTTTCAGC
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=225,22
PRIMER_LEFT_3_TM=60.910
PRIMER_RIGHT_3_TM=60.062
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.9845
PRIMER_LEFT_4_PENALTY=0.792632
PRIMER_RIGHT_4_PENALTY=0.191886
PRIMER_LEFT_4_SEQUENCE=GTCGGGGAAATTCTTCTCATCT
PRIMER_RIGHT_4_SEQUENCE=CATGATTCTCCTATTTTTGGGC
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=147,22
PRIMER_LEFT_4_TM=60.793
PRIMER_RIGHT_4_TM=59.808
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_03696 Ps_contig_mira-and-tgicl-ass_franssen_30752
SEQUENCE=CCCGTTTATTCATTTAAACTATTATTTTTTGTATAGAGTAGATAAAAATATATCGGAGTCAGATTCCACTAAGAAAAATGCTTTCTTAACTAGTGTAGAAAGCTAGGCAAATATGTCCTTGCATTGTTTTTCCACAACACTTATTGACATGGAACATGAATAACAAGCTGAAAACTACAGCATGTTCTAAAGAAAACAAATAGGAACAAGGGAATAAATTTATAGACACTAACTCTAGAGTTCTAAACTCATCCTTGGAGAGATGAAAAACAAGGAAACAACTTTAAGTTACCATCTCTACATCATCCCCTTGCCTGTCAAAAAAGAGAGAGACAAAACCACACCAGCTAAACAAATAATATCTGCCCTTCCAACTTTAGTCTGATAGTCTGATCTCCACTCCTAAAATGTCCTTCACCATCTCATATGATACAAATGCAAGTGCTATGGATGGGACCACCTTTACAGAATTGGGCACCAGACCCTTGTATAATGCACCGAAGCCCTCGTGCCGAACAGTTTTCCTAAATGCATCAATCATTCCAGTATATTCAAGTGGGGCTTTGCCTCTTCCATCTCCAGTTATAACAGAAGCAGCATTGTGCCAACCGGTCATCTGCATTCTACGACGAATGACATCAAGAGGGTACGCGACGGTTTGTCCAATGGTTCCGGCAGCAGCACCACAAGCCAGGCGTGTTGTCACACTCAGCTCAGAATCTTGAACTAGACCAAATGGTCTAGCTTTAATCAACCAATCTTTCAGAGACTCGTAGACAGCAAAGTTGAGACCAACATAAGGAACAACTCCAATTACTGAAGGAAGCCAGCCCTTATATAAAGCACGTGGACCTTCTTGCCTAAGCACAGTAGTCAAAGCATGGAACATTCCTCGGTACTGATAAGGGGATTTCTCGGTCTGTACAGTTATCCTGCCTCGGACCATATCCATTGGATAAGTTGCAGACATAGCAATTATTCCAGCACATGCTCCAGCTCCAAGACGTAAAACAGGGGTAAGCTGAGCATCCTCATTTCCAGTTTGCTGCTGATAGAGTTGCAGTATGCCTTTAGAAGCTTGCTCATAACTGAAGAACTTCACAGCAGAATTTGGGACAATTCGAGCACAGTTAGTACCATTTCCCTTGAACAGTCCACGGAATCCTTCAGTTCTCCATATATATTTTAGGCCTTGTATTGTTCCATTGTATTTTATGTTATGAGGATTCTGGACCTGAAGCAAAATTTTCATTCGCTCGAGTGGAGCAACTGCCGTTCGTGATACTCCTCCTGCGACTCCACCGGCGACGAGAGACTTGCAGATGCTACCGATGGCATAGTTAGGAGCTTTAACAACTCCTTCTCTAGCGAGCTTCGCTTCTTCCGCCAAATTAACGATCTTCGTTACAGCTGATTCACCAGTTTTCACATCTTCCGAAGCCATGCTCAATAACAATAATTCCGATAAAATCTATAAGCCCTAAATCAACCGGAACAAAAAATTTTTTATAAAAAATAGAGAAAATAATCTAGGTCGTCGGAGATTGAGAGATCTTCAATTTTCCGATAAATAACCGGCGAATTATTCCGGCGAAAAACGAAGCTACGCGATCAATTCTCTCTAGCTCGCTCTGAAATGGTGGCTGCGGAGGCTTCTAGAGAGAGAGAGAGAGTGAAAGCTCACAGAGACCACTAAGCTACTACGAGTATGAGCGAAGACGCCGTCCCCGNGTACTCTGTCGTTGACTACCAC
TARGET=1658,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4480
PRIMER_LEFT_PENALTY=0.013714
PRIMER_RIGHT_PENALTY=1.434248
PRIMER_LEFT_SEQUENCE=GAAGCTACGCGATCAATTCTCT
PRIMER_RIGHT_SEQUENCE=GCGTCTTCGCTCATACTCGTAG
PRIMER_LEFT=1599,22
PRIMER_RIGHT=1721,22
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=61.434
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=1.4480
PRIMER_LEFT_1_PENALTY=0.013714
PRIMER_RIGHT_1_PENALTY=1.434248
PRIMER_LEFT_1_SEQUENCE=AAGCTACGCGATCAATTCTCTC
PRIMER_RIGHT_1_SEQUENCE=GCGTCTTCGCTCATACTCGTAG
PRIMER_LEFT_1=1600,22
PRIMER_RIGHT_1=1721,22
PRIMER_LEFT_1_TM=60.014
PRIMER_RIGHT_1_TM=61.434
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=1.5298
PRIMER_LEFT_2_PENALTY=0.095574
PRIMER_RIGHT_2_PENALTY=1.434248
PRIMER_LEFT_2_SEQUENCE=AATCTAGGTCGTCGGAGATTGA
PRIMER_RIGHT_2_SEQUENCE=GCGTCTTCGCTCATACTCGTAG
PRIMER_LEFT_2=1526,22
PRIMER_RIGHT_2=1721,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=61.434
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=1.6029
PRIMER_LEFT_3_PENALTY=0.168661
PRIMER_RIGHT_3_PENALTY=1.434248
PRIMER_LEFT_3_SEQUENCE=TATAAGCCCTAAATCAACCGGA
PRIMER_RIGHT_3_SEQUENCE=GCGTCTTCGCTCATACTCGTAG
PRIMER_LEFT_3=1472,22
PRIMER_RIGHT_3=1721,22
PRIMER_LEFT_3_TM=59.831
PRIMER_RIGHT_3_TM=61.434
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=1.6060
PRIMER_LEFT_4_PENALTY=0.171757
PRIMER_RIGHT_4_PENALTY=1.434248
PRIMER_LEFT_4_SEQUENCE=CTCTAGCTCGCTCTGAAATGGT
PRIMER_RIGHT_4_SEQUENCE=GCGTCTTCGCTCATACTCGTAG
PRIMER_LEFT_4=1619,22
PRIMER_RIGHT_4=1721,22
PRIMER_LEFT_4_TM=60.172
PRIMER_RIGHT_4_TM=61.434
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_03697 Ps_contig_mira-and-tgicl-ass_franssen_30757
SEQUENCE=ACAGTCGRTAATTATCTGATTGAGAAGTAAGAATAGGGAAAGAAAAGAAGAAAGGAAATGGGAAGGATTGTATGGATCCTCATCTCTCTCTCACTGTCTCTTCTCGCTACTCTCGTTAGATCTGATTCCTCCGATCACCGTTACACCGACGGAGATCCCGTTCCTCTATATGCCAACAAAGTCGGTCCGTTTCACAATCCCAGTGAAACGTATCGTTACCTTGACCTTCCATTTTGTGTAACAGGTCATGAGAAAGAGAAGACCGAAGCGCTTGGCGAGGTGTTGAATGGAGATCGGCTTGTTAGTGCTCCGTATGAGCTTCATTTTAGGAAGGAGAAAGACTCTAATGTTGTCTGCAAGAAGAAGCTTACAAGGGAGGAAGTTGCTCAGTTCCGTGAAGCGGTTAAGAAGGATTATTATTTCCAAATGTATTACGATGACTTGCCTATTTGGGGATTTATTGGTACCGTTGACAAAGAAGGAAAAAGTGACCCTAGCGAGTATAAGTATTTTCTTTATAAGCATATTCAGTTTGATGTTTTGTATAATAAAGATCGTGTGATTGAAATTAGTGCTCGTATGGATCCTCATTCTGTGGTGGATGTGACGGAGGACAAGGAAGTTGATGTTGAGTTTATGTATACTGTGAAATGGAAGGAAACAGAAATTCCTTTTGAGAAGAGAATGGAAAAATACTCACAATCTTCTTCTCTCCCGCATCACTTGGAGATTCACTGGTTCTCCATCATAAACTCATGTGTAACGGTTCTTCTTTTGACTGGTTTTCTGGCAACCATTCTCATGCGTGTCTTGAAGAATGATTTTATGAAATATGCTCAAGACGAGGAAGCGGCTGATGATCAAGAGGAGACGGGATGGAAGTACATTCATGGTGATGTTTTCCGGTTTCCTAAGTACAAGTCCATATTCGCGGCTGCCCTTGGTTCTGGCACTCAGTTATTCACTCTCACAATCTTCATTTTTATGCTTGCACTGGTTGGTGTATTCTATCCTTACAATCGAGGAGCTTTATTTACTGCACTGGTGGTTATATATGCTCTCACATCTGGCATTGCTGGCTATACTGCCACTTCCTTCTATATTCAACTTGAAGGGACTAATTGGGTTAGAAACTTATTGCTGACAGGATGCCTCTTCTGTGGCCCTCTTTTCCTTATGTTCTGCTTCCTTAACACAGTTGCAATTGCTTATAGTGCAACTGCTGCCCTTCCTTTTGGCACAATTTGTGTGATAGTCCTAATATGGACGCTTGTAACTTCGCCATTACTTGTGTTGGGTGGCATTGCAGGTAAAAATAGCAAAACAGAGTTCCAAGCACCTGTCCGCACTACAAAATATCCCCGAGAAATTCCACCTCTGCCTTGGTACCGAAGCACCCTTCCACAGATGGCAATGGCAGGATTTCTCCCTTTCAGTGCCATCTACATAGAGTTGTACTACATTTTTGCTAGTGTGTGGGGCCACAGGATTTATACCATCTACAGCATACTCTTCATCGTCTTTATTATTCTGTTGATTGTCACTGCTTTCATTACTGTGGCTTTGACATACTTTCAACTCGCCGCGGAAGATCATGAATGGTGGTGGAGGTCTTTTCTTTGTGGTGGATCAACCGGATTATTCATCTATGGCTACTGCTTGTATTATTACTATGCACGGTCAGACATGTCTGGTTTTATGCAAACTTCGTTCTTCTTCGGTTACATGTCCTGCATCTGCTATGGTTTCTTTCTCATGCTCGGTAGCGTAGGTTTCCGTGCTTCCCTGCTCTTTGTCCGCCACATATACAGGTCCATCAAGTGTGAGTAGCCGTGGTTGAACGTTATGGTAAGTTTACATTATTGGTGTTAGGTAATGAGGAAAGGTCTATGAATTTGTTTTCCTTTTTTCCTGTCATAAGGTAGATTATACAGTGTACCTGATAACAAGAGAAGCTTTGACTCTTCTGAAAATTAGTGTCATGTTATCTCTCTTGTTTAGTTTGCACAGGCTTGACACTTGAGGAAGATTTTTGAACCATGGGATTTAAATGTTTAATTACTGTAGTTACTGAAATTTATTTGATGTAGATTGTACTAATTAATATTTCTACCTGCTTTATGATATNTACCATTCAATACC
TARGET=83,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03698 Ps_contig_mira-and-tgicl-ass_franssen_30762
SEQUENCE=ACAGAACCAACGAACCTCTCGCTCTTTTTTATTTATTTTCACATTTTTTTCATAATCATAAAATCAAATCAATTTTTGTTTCGCTTCTCACACTTCCCGGAACCTTCTCTTCACTCCTTTCACCAATTTTCCGTTGCCATGGTTTCTCTCTATCACATCATTTTCATCTTTTTCATTTCATATACTCCTATATGATATATCAACCTTTCCTCTTTTCCTCTTATTATAATTAATTTTAATCTCAAACTAAACTCATTTAGTTATTAATTTTTTTTTGGTATTAGCGAGAAAGAAATGAGATGAAGCCGATTCAACTTCCACCTCCAACCGCCACCGGCTCAGCTGCAAGTTCTCCTAACCGTCCCCAGCGCCGCCGCAGAGACCTTACGCTTCCGCTTCCACAGCGTGACACAAACTTAGCGGTTCCGCTTCCGCTTCCTCCGTCTGGTGGCGGAAGCGGCGGGAGTGGAAATGGAGGAAGCGGTGGGAACGGAAGTGCGGGAGGAACGAATCAGCAACTGGTGATTCCTTTCTCGGAGCTGGAGAGGCTGAACCGGATCGGAAGCGGTAGCGGAGGAACGGTTTATAAAGTAGTTCACAGAATCAACAGCCGTGCTTACGCTTTGAAGGTGATTTACGGTCATCACGAGGAATCTGTTCGTCGTCAGATCCATAGAGAAATCCAGATCTTGAGAGATGTTGATGATCCAAACGTGGTCAAGTGTCACGAGATGTACGATCACAACGCTGAAATTCAGGTTCTGTTGGAATACATGGACGGTGGTTCTCTCGAAGGGAAACACATCCCTCAGGAGAATCAGCTTGCTGACGTGGCACGTCAGATCCTCCGTGGACTTGCTTATCTCCACCGTCGTCACATCGTTCATAGAGACATCAAACCGTCAAATCTGTTGATCAATTCGCGGAAACAGGTTAAGATCGCTGACTTCGGTGTTGGTAGAATTCTGAATCAAACGATGGATCCGTGTAATTCATCGGTAGGAACGATTGCTTACATGTCACCGGAGAGGATAAACACCGATATCAACGACGGACAATACGATGCCTACGCCGGTGACATATGGAGTTTAGGTGTGAGTATATTGGAGTTTTACATGGGAAGATTTCCGTTTGCGGTTGGTAGACAAGGTGATTGGGCAAGTTTGATGTGTGCTATTTGTATGTCGCAACCACCGGAAGCTCCGACAAGTGCTTCGCCGGAGTTTAGAGATTTCGTTTCGCGGTGTTTGCAGAGAGATCCGTCGAGGAGATGGACTGCTTCGAGGTTGCTTTCGCATCCTTTTCTTACTCGAACGGTTTCTCATCAAAATCAGAGTCCTCCAAATCTCCATCAACTACTTCCTCCTCCACCAAGGCCACTTTCTTCTTAGTTCATGTTTTTTTTTTAATTATTATTATTATTATTATTACTAGTACTATTTTCAAAAAAAGAAGAACTAAATTGGTTGAATCAGATGCTGAGATTGTTACCATAACATGAATGATGAATCTAGCACTGGTGACCTGTTTTTAATTTTATTTTTATCTTTATAAATGTTATATTATGTACCAAAATGTAATCCAAATCCAACGCTTCCTTTATTTGCTTAAAATTATGTAACTGCTTTTCACTTCTTTTGTTTTGTTAGTTATCTTTTTATGCTAAATGATTGTGCACGTTTGGTTAAGTTAAAGGGAATG
TARGET=1409,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3864
PRIMER_LEFT_PENALTY=0.073866
PRIMER_RIGHT_PENALTY=0.312496
PRIMER_LEFT_SEQUENCE=AGAGTCCTCCAAATCTCCATCA
PRIMER_RIGHT_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_LEFT=1332,22
PRIMER_RIGHT=1526,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=60.312
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.3864
PRIMER_LEFT_1_PENALTY=0.073866
PRIMER_RIGHT_1_PENALTY=0.312496
PRIMER_LEFT_1_SEQUENCE=CAGAGTCCTCCAAATCTCCATC
PRIMER_RIGHT_1_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_LEFT_1=1331,22
PRIMER_RIGHT_1=1526,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.312
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=0.3864
PRIMER_LEFT_2_PENALTY=0.073866
PRIMER_RIGHT_2_PENALTY=0.312496
PRIMER_LEFT_2_SEQUENCE=ATCAGAGTCCTCCAAATCTCCA
PRIMER_RIGHT_2_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_LEFT_2=1329,22
PRIMER_RIGHT_2=1526,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=60.312
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=0.3864
PRIMER_LEFT_3_PENALTY=0.073866
PRIMER_RIGHT_3_PENALTY=0.312496
PRIMER_LEFT_3_SEQUENCE=TCAGAGTCCTCCAAATCTCCAT
PRIMER_RIGHT_3_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_LEFT_3=1330,22
PRIMER_RIGHT_3=1526,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=60.312
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=0.4348
PRIMER_LEFT_4_PENALTY=0.122257
PRIMER_RIGHT_4_PENALTY=0.312496
PRIMER_LEFT_4_SEQUENCE=CATCCTTTTCTTACTCGAACGG
PRIMER_RIGHT_4_SEQUENCE=CAGGTCACCAGTGCTAGATTCA
PRIMER_LEFT_4=1295,22
PRIMER_RIGHT_4=1526,22
PRIMER_LEFT_4_TM=60.122
PRIMER_RIGHT_4_TM=60.312
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_03699 Ps_contig_mira-and-tgicl-ass_franssen_30772
SEQUENCE=AAAAAAGAAAACAAAAGTAAAGGTAAATAACAAACGACAGAGTACGCGGGGACTCCACACACTCACTCACTCAAAATTCCTTAACCATGATCAACATTCCAAACCTTAAAATCCCCGAATGGGACACGACACAATACGAGGGAGGAGCATTCTTAAACCATTTCATTTCAAACGACTCCGACACACAATTTTCCATTACCGAAGATTTCGACTTACCTCTTCATGTTTTCTCTACCGACCATTTCCGAATGTTCGAATTCAAAGTGAGGAAATGCCAGCGTGGCAGGTCTCATGACTGGACGGAATGTCCTTACTCTCATCCCGGCGAGAAAGCTCGCCGGAGAGACCCTAGGAAGTTTAATTACTCCGGTACACCTTGCTCGGAGTTTCGCAGATCAGGAAACTGTACTAAAGGTGATTCATGTTACTTTGCGCATGGTGTTTTCGAATGCTGGCTTCATCCTTCTCGTTACAGGACACAGCTTTGTAACGATGGAACTGCTTGTCGCCGGCGAGTGTGTTTCTTTGCTCACACCATTGATCAACTTCGTCTCTCGGGTAAGGCTTCACCGGAATCATTTGTTTCATCTCCTACTTCGGTTCTTGAATCTCCTCCGGGAAACTCCCGATACGGCGACGTTCCGGTGAATATGCGGGAACTAGTTGACTGCATGAGAGATGTTCGAATTGCGGATTCGGGTCGGGTCGGGTGTCCGCAGGGTGGGTTTTTTAGTCTTCCGGCAAGCTATGAAGGGGTTAGTATGGAGAGGGTGGAATCTGGAAGAGATCTTAGAGCCAAAATCTATGGAAAATTCAGTAAGGTGAACTTCAACGACGGTGTTGTTTCGGTTCCTGTTCCTGATTTTGGATGGGTTTCGGAACTGGTTAACTGAATTTGGGATTGAGGTTGAGGAGTATGATTCTGCTTTGTATATAGGATGAATGATGCAAAAAGGAAGAAGAATCTTTGTGCATAGTTGAGGAGCATGAGATGAGACTATGGTGTCTCTGTCATGTCCATTTTCTAACGTTTAGTTTTCTTCTTGAGTTAAGGATTTGTTGAAGATTGGATAACATTTGATTGTCTTCATTGTTCTTTTTTACCTGGATTTTTGTCTTCATCTTTTGTTGTGTTGGATGTTTATATGTCAATAGAAGAAACATGAACAATGATGTATTTTTATTTCTAAAAATTATGGGTATTTTTTATTATTTCTAGAATGAATATGAATCGTAATACCTTCTTTACT
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.6880
PRIMER_LEFT_PENALTY=4.653897
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=GGTAAATAACAAACGACAGAGTACG
PRIMER_RIGHT_SEQUENCE=AGTCGAAATCTTCGGTAATGGA
PRIMER_LEFT=21,25
PRIMER_RIGHT=212,22
PRIMER_LEFT_TM=58.346
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=4.9661
PRIMER_LEFT_1_PENALTY=4.653897
PRIMER_RIGHT_1_PENALTY=0.312177
PRIMER_LEFT_1_SEQUENCE=GGTAAATAACAAACGACAGAGTACG
PRIMER_RIGHT_1_SEQUENCE=GTCGAAATCTTCGGTAATGGAA
PRIMER_LEFT_1=21,25
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=58.346
PRIMER_RIGHT_1_TM=60.312
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=5.0159
PRIMER_LEFT_2_PENALTY=4.653897
PRIMER_RIGHT_2_PENALTY=0.361969
PRIMER_LEFT_2_SEQUENCE=GGTAAATAACAAACGACAGAGTACG
PRIMER_RIGHT_2_SEQUENCE=AGAGTAAGGACATTCCGTCCAG
PRIMER_LEFT_2=21,25
PRIMER_RIGHT_2=316,22
PRIMER_LEFT_2_TM=58.346
PRIMER_RIGHT_2_TM=59.638
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=5.0447
PRIMER_LEFT_3_PENALTY=4.653897
PRIMER_RIGHT_3_PENALTY=0.390771
PRIMER_LEFT_3_SEQUENCE=GGTAAATAACAAACGACAGAGTACG
PRIMER_RIGHT_3_SEQUENCE=TGGTCGGTAGAGAAAACATGAA
PRIMER_LEFT_3=21,25
PRIMER_RIGHT_3=240,22
PRIMER_LEFT_3_TM=58.346
PRIMER_RIGHT_3_TM=59.609
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=5.0890
PRIMER_LEFT_4_PENALTY=4.653897
PRIMER_RIGHT_4_PENALTY=0.435099
PRIMER_LEFT_4_SEQUENCE=GGTAAATAACAAACGACAGAGTACG
PRIMER_RIGHT_4_SEQUENCE=GGTTTAAGAATGCTCCTCCCTC
PRIMER_LEFT_4=21,25
PRIMER_RIGHT_4=158,22
PRIMER_LEFT_4_TM=58.346
PRIMER_RIGHT_4_TM=60.435
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_03700 Ps_contig_mira-and-tgicl-ass_franssen_30772
SEQUENCE=AAAAAAGAAAACAAAAGTAAAGGTAAATAACAAACGACAGAGTACGCGGGGACTCCACACACTCACTCACTCAAAATTCCTTAACCATGATCAACATTCCAAACCTTAAAATCCCCGAATGGGACACGACACAATACGAGGGAGGAGCATTCTTAAACCATTTCATTTCAAACGACTCCGACACACAATTTTCCATTACCGAAGATTTCGACTTACCTCTTCATGTTTTCTCTACCGACCATTTCCGAATGTTCGAATTCAAAGTGAGGAAATGCCAGCGTGGCAGGTCTCATGACTGGACGGAATGTCCTTACTCTCATCCCGGCGAGAAAGCTCGCCGGAGAGACCCTAGGAAGTTTAATTACTCCGGTACACCTTGCTCGGAGTTTCGCAGATCAGGAAACTGTACTAAAGGTGATTCATGTTACTTTGCGCATGGTGTTTTCGAATGCTGGCTTCATCCTTCTCGTTACAGGACACAGCTTTGTAACGATGGAACTGCTTGTCGCCGGCGAGTGTGTTTCTTTGCTCACACCATTGATCAACTTCGTCTCTCGGGTAAGGCTTCACCGGAATCATTTGTTTCATCTCCTACTTCGGTTCTTGAATCTCCTCCGGGAAACTCCCGATACGGCGACGTTCCGGTGAATATGCGGGAACTAGTTGACTGCATGAGAGATGTTCGAATTGCGGATTCGGGTCGGGTCGGGTGTCCGCAGGGTGGGTTTTTTAGTCTTCCGGCAAGCTATGAAGGGGTTAGTATGGAGAGGGTGGAATCTGGAAGAGATCTTAGAGCCAAAATCTATGGAAAATTCAGTAAGGTGAACTTCAACGACGGTGTTGTTTCGGTTCCTGTTCCTGATTTTGGATGGGTTTCGGAACTGGTTAACTGAATTTGGGATTGAGGTTGAGGAGTATGATTCTGCTTTGTATATAGGATGAATGATGCAAAAAGGAAGAAGAATCTTTGTGCATAGTTGAGGAGCATGAGATGAGACTATGGTGTCTCTGTCATGTCCATTTTCTAACGTTTAGTTTTCTTCTTGAGTTAAGGATTTGTTGAAGATTGGATAACATTTGATTGTCTTCATTGTTCTTTTTTACCTGGATTTTTGTCTTCATCTTTTGTTGTGTTGGATGTTTATATGTCAATAGAAGAAACATGAACAATGATGTATTTTTATTTCTAAAAATTATGGGTATTTTTTATTATTTCTAGAATGAATATGAATCGTAATACCTTCTTTACT
TARGET=696,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3201
PRIMER_LEFT_PENALTY=0.124655
PRIMER_RIGHT_PENALTY=0.195462
PRIMER_LEFT_SEQUENCE=CATTGATCAACTTCGTCTCTCG
PRIMER_RIGHT_SEQUENCE=CCCTCTCCATACTAACCCCTTC
PRIMER_LEFT=535,22
PRIMER_RIGHT=770,22
PRIMER_LEFT_TM=59.875
PRIMER_RIGHT_TM=60.195
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.3258
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.195462
PRIMER_LEFT_1_SEQUENCE=ATCAACTTCGTCTCTCGGGTAA
PRIMER_RIGHT_1_SEQUENCE=CCCTCTCCATACTAACCCCTTC
PRIMER_LEFT_1=540,22
PRIMER_RIGHT_1=770,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.195
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.3495
PRIMER_LEFT_2_PENALTY=0.154023
PRIMER_RIGHT_2_PENALTY=0.195462
PRIMER_LEFT_2_SEQUENCE=GTTTCTTTGCTCACACCATTGA
PRIMER_RIGHT_2_SEQUENCE=CCCTCTCCATACTAACCCCTTC
PRIMER_LEFT_2=519,22
PRIMER_RIGHT_2=770,22
PRIMER_LEFT_2_TM=60.154
PRIMER_RIGHT_2_TM=60.195
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=0.3538
PRIMER_LEFT_3_PENALTY=0.158317
PRIMER_RIGHT_3_PENALTY=0.195462
PRIMER_LEFT_3_SEQUENCE=ACAGGACACAGCTTTGTAACGA
PRIMER_RIGHT_3_SEQUENCE=CCCTCTCCATACTAACCCCTTC
PRIMER_LEFT_3=471,22
PRIMER_RIGHT_3=770,22
PRIMER_LEFT_3_TM=59.842
PRIMER_RIGHT_3_TM=60.195
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.3610
PRIMER_LEFT_4_PENALTY=0.165541
PRIMER_RIGHT_4_PENALTY=0.195462
PRIMER_LEFT_4_SEQUENCE=TTTGTAACGATGGAACTGCTTG
PRIMER_RIGHT_4_SEQUENCE=CCCTCTCCATACTAACCCCTTC
PRIMER_LEFT_4=483,22
PRIMER_RIGHT_4=770,22
PRIMER_LEFT_4_TM=60.166
PRIMER_RIGHT_4_TM=60.195
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_03701 Ps_contig_mira-and-tgicl-ass_franssen_30776
SEQUENCE=AATAAGCTTATTAATTTTTTTTATTAATTACCGCACGATTTCCAGGCCGTGAATATTATAAATAGATAAATTTATAATCACAAAGCAAAGCTGAAAAAAAAAAGTACCAGCAACATTATCCTGTGATGCCGCTACACTGAATCGCGCTCGGAATATTCCTCTTCCGGAAAACCGTTGAACACTCCGGCAACTCCCCGAGATCAGCAAACAGCGATTCATCCTCTTCTCTCATCGTCAGCGTTATATCCTCCACTCCTCCGCAGATCGGACTTACAAGAACTCCGGTAGATGCAACGTCGTCGAACCAGCCAAACACGGCATGGTGGTGGTGGTGGTGGCTGAGCAGAACAGTCGAGTCACCGGAGAGGTCAAAGTCAGGATGAGTGGCGAAAACCGTTAGATCCTCTTGCTGTGGAGTGGCGGCGGGAGAGGCAGTGTTGCATTCACCGGAAGAATCGCCGGGAGATGGAGATGGAGATTCGACGGTGACGGTTACGGCGGTGGAAGAGGAAGAATGAGATTTGGGAAGAGGGAGAGAGTGGTTGTGGTCATGGGCGTAGGTTACGAGAAGCTTTGTGGGGTCCACGCGGCTTCTCTCAACTTGTTTCCTAGCGGGACACCCTTTTGAACTGCTACATCGGTAATATCCCCTGGGATAAGGGGAGCCTTTGATGGGCTTTTGACCGTACTTTCTCCAGGCCCATGAATCTGACGGTGGACACGTCTCTCCTTTGCTCTTAGATCCTTCCACGTCAGCAATCGGTATAGTCACCACCCTCTTCTTCATCTCCCTCCTTTTCTTAGGCGACGGTTCTTCAATTTTTGCGTCTTCACAAGAAGGAGAAGCAGGCTCAGTTTCCGAGATGGGCTCGGGCTCGGGCTCGAGGCTCATCRRGGTCAACTAACATACATTGGTTTAGTTGTTAGAATTCGTGGTTCGTTT
TARGET=321,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03702 Ps_contig_mira-and-tgicl-ass_franssen_30780
SEQUENCE=ATCCCAAAACAACCCATTTCGGGGATATCCTTTTTTTCTCTCTTCTACTTCTTCTCTCTATTTCTTCATCATGGCAGTTGAGGCTCGCCATCTTCACCTCTTTTCCCCTCAACTCATAAACAACAGAGAAATGATGAACCCTGTTGAAACAAACGTCAACAACATCTACAACAACATGGGTTATTCCTCTGTTCCTCCGTCTTCTATCAAAACCGCCGCGACGGAGGCTCTTATTCTTCCGCCGTACAACTCTATAACCGCTGATTCGTTACCTCAGAAAACTGCTATGAACTCTGATAGTGGTCTCACTTATAACGTTCCCTTGAGAAAACGTTCTAGAGATTCCAGAGACTATTCAAATTCAATCAATTTCGCTTTTCAGAATTCTTATCTTCCTCCTTCAACAGCGCCACAGAACAACAAAAGTTGTGCCTCTTCCTCGTTCTCTTTTCTTGGTGAAGACATTTCCCTTCAGATCCAAAGGCAACAACTTGACATCGATCAACTCATTGCACAACAAATGGAGAAGGTGAAATATGAGATTGAAGAGAAGAGGAAGAGACAAGCGATGAGGCTGATTCAAGCTATTGACATGACTGTTACGAAGAGATTGAAGGCTAAAGAAGAAGAAATCGAGAAAATTGCTAGAACTAATTGGGCTTTAGAAGAAAGAGTTAAGTCACTTTGCATGGAGAATCAAATATGGCGTGATTTGGCTCAAAGCAATGAAGCCACAGCCAATGCTCTGCGCTCTAATCTCGAACAGCTTCTTGCACAGCGAGTCGGAGATGATAATGGGGATAGTATTCCTCCGGTCGGTCGGCACGCTGCTCAAATCGATGACGCCGAGTCTTGTTGTGATAGCAATGATAGTATCAATGAGGAAGAGAATGTTGTGGAATGGCGAAGTATAGGTGATAATGATAACAATGGTAGAAACATTGAAGCAATGAAGATGATTAGAAACTGTGGTGGTGGTGGTGGTGGCGGTGTTGATAGTGGAAGTGGTGGAAGCAATTTTGTGAATAGCATGAAACTATGCAGCAACTGTGGGAAAGATGAATCTTGTGTGTTGATTTTGCCTTGTAGACATCTCTGTTTATGTGCTGTTTGTGGGTCTAATCTTCATATTTGTCCCATTTGTAAATCTTTCAAAACTGCTAGTATCCATGTTAACATGTCATGAACATGAACATGAATCAATTACTTTAGTGAGACTTTAGTTTCTTAGAGAAAAAAAAACTTTAACTAAGAATGAATGGTAAAAAAAAAGTACAYATTTGTATCCARNRTCACTTGTTGTTTTTTTGTTTTGTTTGGTTTTAGTTACATTACTTTAATCCATTTTTAGACGATTATTACATCGTTGTATTATTGCAGTTTTTGCTTTGTTTCATTAGGAAGGTAATAAAAAAAGTAGAAAACTAAAATAAA
TARGET=969,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03703 Ps_contig_mira-and-tgicl-ass_franssen_30781
SEQUENCE=AACAAAACATTTTACTTCCTTTTTACNATAACAAATACGATACGCATTAACGCAACACAAGAACAAACAAACCAACCAACCAGTCCAAAATGGAGCACGACGAAGACGAAGAGCCACCTCTTGCTGTTCAGATTCAACGAGATGATGATCAATCCATTTCTCAACAATCTTCTGTTGGTGTTACTCTCATCACCGGCTACCTTGGTTCCGGCAAGTCCACGCTAGTGAATCATATATTGAACTCTCAACATGGGAAGAGGATTGCTGTCATATTGAATGAGTTTGGGGAGGAAATTGGAGTAGGAAAGAGCATTGATAAATGAAGGGGAAGAAGGTGCAGTTGTTGAAGAATGGGTTGAGCTTGCTAATGGGTGTGTATGTTGCACGGTTAAGCATAGTTTGGTTCAAGCCCTTGAACAGCTTGTGCAGAGAAAAGAAAGGCTAGACCATATATTGCTGGAAACTACTGGTTTGGCTAATCCAGCACCTTTGGCATCTATTCTTTGGTTGGATGAGCAGTTGGAATCTGACGTGAAGCTTGATTCTATCGTCACTGTAGTAGATGCCAAAAATGTTCGCTTTCAGCTCAAGGAGCACCGTGGCTCATCGTCATTTCCTGAAGCATATTTTCAGATAGCATTTGCGGACATTGTAATTCTTAACAAGGTTGATTTGGTATCTGCAGAAGGCTCTGGAGCTCTGGAGGAACTCGAGGAGGAAATCCATAACATTAACTCACTCGTAGAGATAATTCACTCTGTCCGGTGTCAAGTTGACTCATCTAAGATATTGAACCGCCACGCTTACGATACTCATGCTGCACATTTAGAGGCATTGTTAGAAGAAAGTCGTTCTTTGTCTTCTAATAAGCTTCATGATAGTGGCGTGAGAACCCTATGCGTTTGTGAGACGGGCACAGTTGATATTGATAAGACTCGTATATGGCTAGAAGAAATTCTTTGGGAGAAGAAACATGACATGGATGTATACCGTTGCAAAGGAGTCTTAAGTGTTCAAAACTCTGATCAGTTGCATACTTTGCAGGCGGTGAGGGAACTATATGAGATTGTTCCTGCTCGCAAGTGGAAGAAGGAAGAAAACAGGGTGAATAAGATAGTCTTTATAGGTCATAATCTGAAAGAAGATGTTCTAATTAGTTCCTTGAGAGCTCTTGCAACCTGTTAGGCTGTTTATTTGTATGGGTAAAGACTAATAGCATAAGTATTTATCAGTTTGGAAAAGTGACTTCTTTTTCTTTGGTTTCAAAACATAGTTCGAAATTTAGGCTACTCGCCCTGATTCAGGATTTGGCCGAAAGCAAAAACAAATCAAAGAAACTGTAATCGGAAAAGGCCACTAATGATACTCTTACAAGCCAAGATTAGTAAATTGCATTTGAATGTTGTTATGAGTTGTGTATATACTTTTTCTARNGNYCATCATCTCTTAATATAGTAATTTAATGTGACGGTCCACAATCCGAATGCTTAAA
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03704 Ps_contig_mira-and-tgicl-ass_franssen_30783
SEQUENCE=TTTCGATGTAAGTGAAATCCTTAGAAACAAAGGGGCTGAACAGTAGCCTGATCCCAGTCATAAGAGAATAAACAGGTTACAGCTAGTTTGATAATTATATTGCTCCATAATTCAAACTGTATGTTCCACTATCATTAGAAATGTATAAGCAATTCAATAGATCACCAATACTTTTTACCCTACAAACCATCAAAAAACATATAACCAATTTTCAATGTTTTTCCACTCCTTCAAGGTATCGTTGATACCAGTATCAAACTGGAAGTTCTTCATATGACAGCTTCAAATTCAGAATCACTTTCTTCAGGGGCGGCAGCAGGCACTTTATTGTTGTTGCCTCTCAGACGTGAATATATATTATTAATGGCTGAGTTTAGTTGGCCAATTTTTTCATTGAAGGTCTCCTTGGTACTCTCCTCTATCTCTTCATCATAATATATTGGTCTTCTGGCCCTCTGAAACCCATGCTCATTTTCATCTAGCACAGTTCTTCTGATCTGGGGAGCAAAAACATAAGCCAAGGTTCCAAAAATAGCACCACCCAGAATAAAGCCAGTAAGCAATTCCCCACCTCCACCTCCTCCTCTGTTATCACGGTATTCTGCACGAAGTGGTGAAGTGAGGTTCTTTCTTGTCAGAATGTTTGATTTCCTTTGAACAGCAAGGTTAGGTCGAACCAGAGATGACTTGAGATGAGATCCACCAACTGAAATGGATAATGGCGCGAAAGAAGTCGCCATGGGAGAGAGAGAGTTTGTTGAGAAGCGTGAATGGAAGAGAGAGAGAGAGAGAGAGGTGTTGTAATTTGTATGGAGCTATCGGGTCNCCCGTANGTTAATTAACGTAAGGGAAGGAAGTACG
TARGET=743,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1630
PRIMER_LEFT_PENALTY=0.009871
PRIMER_RIGHT_PENALTY=0.153161
PRIMER_LEFT_SEQUENCE=GCAAAAACATAAGCCAAGGTTC
PRIMER_RIGHT_SEQUENCE=CTCTCTCTCTTCCATTCACGCT
PRIMER_LEFT=504,22
PRIMER_RIGHT=784,22
PRIMER_LEFT_TM=60.010
PRIMER_RIGHT_TM=60.153
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.2609
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.153161
PRIMER_LEFT_1_SEQUENCE=ACCACCCAGAATAAAGCCAGTA
PRIMER_RIGHT_1_SEQUENCE=CTCTCTCTCTTCCATTCACGCT
PRIMER_LEFT_1=536,22
PRIMER_RIGHT_1=784,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.153
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=0.2748
PRIMER_LEFT_2_PENALTY=0.121635
PRIMER_RIGHT_2_PENALTY=0.153161
PRIMER_LEFT_2_SEQUENCE=CAAAAACATAAGCCAAGGTTCC
PRIMER_RIGHT_2_SEQUENCE=CTCTCTCTCTTCCATTCACGCT
PRIMER_LEFT_2=505,22
PRIMER_RIGHT_2=784,22
PRIMER_LEFT_2_TM=59.878
PRIMER_RIGHT_2_TM=60.153
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.3899
PRIMER_LEFT_3_PENALTY=0.236697
PRIMER_RIGHT_3_PENALTY=0.153161
PRIMER_LEFT_3_SEQUENCE=CAAAAATAGCACCACCCAGAAT
PRIMER_RIGHT_3_SEQUENCE=CTCTCTCTCTTCCATTCACGCT
PRIMER_LEFT_3=526,22
PRIMER_RIGHT_3=784,22
PRIMER_LEFT_3_TM=60.237
PRIMER_RIGHT_3_TM=60.153
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.4629
PRIMER_LEFT_4_PENALTY=0.309780
PRIMER_RIGHT_4_PENALTY=0.153161
PRIMER_LEFT_4_SEQUENCE=CCTTTGAACAGCAAGGTTAGGT
PRIMER_RIGHT_4_SEQUENCE=CTCTCTCTCTTCCATTCACGCT
PRIMER_LEFT_4=650,22
PRIMER_RIGHT_4=784,22
PRIMER_LEFT_4_TM=59.690
PRIMER_RIGHT_4_TM=60.153
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=135
=
PRIMER_SEQUENCE_ID=SSR_03705 Ps_contig_mira-and-tgicl-ass_franssen_30783
SEQUENCE=TTTCGATGTAAGTGAAATCCTTAGAAACAAAGGGGCTGAACAGTAGCCTGATCCCAGTCATAAGAGAATAAACAGGTTACAGCTAGTTTGATAATTATATTGCTCCATAATTCAAACTGTATGTTCCACTATCATTAGAAATGTATAAGCAATTCAATAGATCACCAATACTTTTTACCCTACAAACCATCAAAAAACATATAACCAATTTTCAATGTTTTTCCACTCCTTCAAGGTATCGTTGATACCAGTATCAAACTGGAAGTTCTTCATATGACAGCTTCAAATTCAGAATCACTTTCTTCAGGGGCGGCAGCAGGCACTTTATTGTTGTTGCCTCTCAGACGTGAATATATATTATTAATGGCTGAGTTTAGTTGGCCAATTTTTTCATTGAAGGTCTCCTTGGTACTCTCCTCTATCTCTTCATCATAATATATTGGTCTTCTGGCCCTCTGAAACCCATGCTCATTTTCATCTAGCACAGTTCTTCTGATCTGGGGAGCAAAAACATAAGCCAAGGTTCCAAAAATAGCACCACCCAGAATAAAGCCAGTAAGCAATTCCCCACCTCCACCTCCTCCTCTGTTATCACGGTATTCTGCACGAAGTGGTGAAGTGAGGTTCTTTCTTGTCAGAATGTTTGATTTCCTTTGAACAGCAAGGTTAGGTCGAACCAGAGATGACTTGAGATGAGATCCACCAACTGAAATGGATAATGGCGCGAAAGAAGTCGCCATGGGAGAGAGAGAGTTTGTTGAGAAGCGTGAATGGAAGAGAGAGAGAGAGAGAGAGGTGTTGTAATTTGTATGGAGCTATCGGGTCNCCCGTANGTTAATTAACGTAAGGGAAGGAAGTACG
TARGET=776,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6519
PRIMER_LEFT_PENALTY=0.010545
PRIMER_RIGHT_PENALTY=0.641309
PRIMER_LEFT_SEQUENCE=TCGAACCAGAGATGACTTGAGA
PRIMER_RIGHT_SEQUENCE=GACCCGATAGCTCCATACAAAT
PRIMER_LEFT=671,22
PRIMER_RIGHT=824,22
PRIMER_LEFT_TM=59.989
PRIMER_RIGHT_TM=59.359
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.7231
PRIMER_LEFT_1_PENALTY=0.081801
PRIMER_RIGHT_1_PENALTY=0.641309
PRIMER_LEFT_1_SEQUENCE=ACCAACTGAAATGGATAATGGC
PRIMER_RIGHT_1_SEQUENCE=GACCCGATAGCTCCATACAAAT
PRIMER_LEFT_1=701,22
PRIMER_RIGHT_1=824,22
PRIMER_LEFT_1_TM=60.082
PRIMER_RIGHT_1_TM=59.359
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.7491
PRIMER_LEFT_2_PENALTY=0.107763
PRIMER_RIGHT_2_PENALTY=0.641309
PRIMER_LEFT_2_SEQUENCE=ACCACCCAGAATAAAGCCAGTA
PRIMER_RIGHT_2_SEQUENCE=GACCCGATAGCTCCATACAAAT
PRIMER_LEFT_2=536,22
PRIMER_RIGHT_2=824,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=59.359
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.7524
PRIMER_LEFT_3_PENALTY=0.111077
PRIMER_RIGHT_3_PENALTY=0.641309
PRIMER_LEFT_3_SEQUENCE=TTGAGATGAGATCCACCAACTG
PRIMER_RIGHT_3_SEQUENCE=GACCCGATAGCTCCATACAAAT
PRIMER_LEFT_3=687,22
PRIMER_RIGHT_3=824,22
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=59.359
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=0.8780
PRIMER_LEFT_4_PENALTY=0.236697
PRIMER_RIGHT_4_PENALTY=0.641309
PRIMER_LEFT_4_SEQUENCE=CAAAAATAGCACCACCCAGAAT
PRIMER_RIGHT_4_SEQUENCE=GACCCGATAGCTCCATACAAAT
PRIMER_LEFT_4=526,22
PRIMER_RIGHT_4=824,22
PRIMER_LEFT_4_TM=60.237
PRIMER_RIGHT_4_TM=59.359
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_03706 Ps_contig_mira-and-tgicl-ass_franssen_30793
SEQUENCE=TACGTCGGGGTAAGAAGGCTTTCCTGAAAGACAACAATATTAGTTATTTTCATCCATCGTCAATAATGAATAATGAAATATCGCCATAGAATTGAGTCACACACCCTCTCCTTCTGAACAAGTGTCGAATGCATCACGATAAGATATTAGTAAACAACAACAACATCAACAACTTATCATAATTCTCCACACACAATCTAATTCTAATCACCGCCACCATGGCTCTGGACTTCAGTTACATCCTCATAATTGATATTAGTCCTCTTTTAGCCAAATCAGATGACCCAAAAATGGCTCAAGACCCTGAAGTACTTGACGTTGTTGCACAATTGGATAAGGCTTGTACTCAGGCTGGCTTCTTTTATGTGAAAGGCCATGGTATTCCTGATACACTTCTCAAAGGGGTTAGAGATATAACGCGCACATTCTTCGAACTTCCTTATGAAGAAAAGGACAAAAATCAAAATGACACCGGCGAATGGGTTCAGAGGATATGAGAAGATGGGAGAAAATCTAACTGAAGGTACACCCGATATGCATGAAGCTATTGATTGCTATAGGGAAGTGACTAAAGGCATGTATGGAGATCTTGGCAAAGTTATGGAAGGATGCAACCAGTGGCCACAAAATCCTCCGAAGTTCAACCTTGTTATGGAGGAATATATTGGTCTTTGTAAAGATCTTGCGAGGAAAATTATGCGCGGAATTGCTTTAGCATTAGGTGGATCGCCGTATGAATTTGAAGGTGATAGAGCTGGTGATCCATTTTGGGTAATGCGTATAATCGGTTACCCTGGTGTATCAACTGTAAATGACATTGGATGTGGAGCTCACACAGATTATGGTTTATTGACATTGTTGAACCAAGATGACGATATAAATGCACTTCAGGTGAAAAACCTATCTGGCGAATGGATATCCGCATCTCCAGTTCCAGGGTCATTTGT
TARGET=153,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1110
PRIMER_LEFT_PENALTY=0.036173
PRIMER_RIGHT_PENALTY=0.074807
PRIMER_LEFT_SEQUENCE=ATTGAGTCACACACCCTCTCCT
PRIMER_RIGHT_SEQUENCE=AAGAAGCCAGCCTGAGTACAAG
PRIMER_LEFT=90,22
PRIMER_RIGHT=360,22
PRIMER_LEFT_TM=60.036
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.1232
PRIMER_LEFT_1_PENALTY=0.036173
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=ATTGAGTCACACACCCTCTCCT
PRIMER_RIGHT_1_SEQUENCE=CATAAAAGAAGCCAGCCTGAGT
PRIMER_LEFT_1=90,22
PRIMER_RIGHT_1=365,22
PRIMER_LEFT_1_TM=60.036
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.1232
PRIMER_LEFT_2_PENALTY=0.036173
PRIMER_RIGHT_2_PENALTY=0.087031
PRIMER_LEFT_2_SEQUENCE=ATTGAGTCACACACCCTCTCCT
PRIMER_RIGHT_2_SEQUENCE=ACATAAAAGAAGCCAGCCTGAG
PRIMER_LEFT_2=90,22
PRIMER_RIGHT_2=366,22
PRIMER_LEFT_2_TM=60.036
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.1545
PRIMER_LEFT_3_PENALTY=0.079714
PRIMER_RIGHT_3_PENALTY=0.074807
PRIMER_LEFT_3_SEQUENCE=CTTCTGAACAAGTGTCGAATGC
PRIMER_RIGHT_3_SEQUENCE=AAGAAGCCAGCCTGAGTACAAG
PRIMER_LEFT_3=110,22
PRIMER_RIGHT_3=360,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=0.1665
PRIMER_LEFT_4_PENALTY=0.036173
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=ATTGAGTCACACACCCTCTCCT
PRIMER_RIGHT_4_SEQUENCE=TACTTCAGGGTCTTGAGCCATT
PRIMER_LEFT_4=90,22
PRIMER_RIGHT_4=310,22
PRIMER_LEFT_4_TM=60.036
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_03707 Ps_contig_mira-and-tgicl-ass_franssen_30797
SEQUENCE=AACTTTGTACAAAATTTTTACCGAAACCTGAAGCAAAAGTCTTTCGATTACTCCATTGTCAACCAGTTCAAAGAAAAAGCAAAGACAAGTTTGACAAGATAAAAAATCCAACATAAATCCTAGACATGCAGCACCATGGCACAAAAGTGTGCATGTCAGTTAATACAATCCATATAAATAAAAGAAGAAAATATCAAATTATCTAAATGTGACTCAACCAAATCAAACCAAAACAACGAGGAAATTATCCACAAATCAAGTTATCATCAACAAATTTACATCATAATCCATCTTTTTCCGCGCTGATTTGGGCAAATTACAAACCTTCATTCCTTTGAGGACTTGTTGATAAGAGACTTGTGGATATGAGGAATAACACCACCTCCGGCAATTGTTCCTTTGATGAGAGTGTCAAGTTCTTCGTCTCCACGGATAGCAAGCTGTAGATGTCTTGGTGTAATACGCTTCACCTTCAGATCCTTGCTTGCGTTACCAGCCAACTCCAGCACCTCTGCGGTTAAGTACTCCAGGATAGCAGCGGAATAAACTGCAGCTGTTGCCCCGACTCTTCCGTGCGCCATTGTCCTTGACTTCAGAAGACGATGGATACGACCAACAGGGAACTGAAGACCAGCTCTGGAGGAACGAGAAGTGGGTTTCTTCTTGTCCTTGTCTTTGAGGGATGATGGGGTTTTACCAGTTGTCAAACCCTTCGCTCCTTTCCCCGACATTGTTTCTCGCTTCGATCGTTTGAGAGAGAGAGAGTTTTGGCTTTTGAGATTTTGCTTCTATGTTACCCTYATGTCTCTTTTTATTCTCAAAATATAT
TARGET=753,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03708 Ps_contig_mira-and-tgicl-ass_franssen_30799
SEQUENCE=AGTCGTAAAAAGACGAGATTCTTCAATTTCCCCAATTGTAAGGTGGGATGGTTCCCGATATATTGTTGTTATACAAGTCCAAGCTAACAAGGCTCTTGAGGTTTCCAAGCTCTGGAGGAATAGTTCCTTGAATGTTGTTTTTGTACAGCTCGAGGTACTGCAGATGCTCTAGCTTCCCAAGTTCAGGTACCAAATGTCCAGATAGGTTAGAATTACCAAGATCCACTCGAGTGACCCGATTGTCTTGGTTACAGGTGACATGGAACCAAGTACAAGGACTGACTAGGGTCGGATCCCAGCTCTGGAGGATATTATCAGGGTCGGTGAGGCTTCGTTTGAGTGTGTAAAGAGCGTCTCCTTCAGAATTAGCATGTGCGAAGTGAGTTAGGGTTAGGGTTAGGGTTAGGATTAGGGGAATTCCGAAAAGGGAAAGTGAAGTGAAGATTGGTGCCATGTTCTTCACTGATCTGAGGTTTGTTGGATTGAACAGAAAACGCGTGTTAGGGTTCGTTCTTATGAGAGAGAGAGAGGGAAATGGGAAATGGGAAAATCGGGAANTAGGAAGTAAGG
TARGET=518,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2580
PRIMER_LEFT_PENALTY=0.029253
PRIMER_RIGHT_PENALTY=0.228700
PRIMER_LEFT_SEQUENCE=TGATCTGAGGTTTGTTGGATTG
PRIMER_RIGHT_SEQUENCE=ACTTCCTANTTCCCGATTTTCC
PRIMER_LEFT=463,22
PRIMER_RIGHT=565,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=59.771
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=103
PRIMER_PAIR_PENALTY_1=0.2895
PRIMER_LEFT_1_PENALTY=0.060824
PRIMER_RIGHT_1_PENALTY=0.228700
PRIMER_LEFT_1_SEQUENCE=ATGTGCGAAGTGAGTTAGGGTT
PRIMER_RIGHT_1_SEQUENCE=ACTTCCTANTTCCCGATTTTCC
PRIMER_LEFT_1=370,22
PRIMER_RIGHT_1=565,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=59.771
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=0.3178
PRIMER_LEFT_2_PENALTY=0.089133
PRIMER_RIGHT_2_PENALTY=0.228700
PRIMER_LEFT_2_SEQUENCE=GAATTAGCATGTGCGAAGTGAG
PRIMER_RIGHT_2_SEQUENCE=ACTTCCTANTTCCCGATTTTCC
PRIMER_LEFT_2=362,22
PRIMER_RIGHT_2=565,22
PRIMER_LEFT_2_TM=59.911
PRIMER_RIGHT_2_TM=59.771
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.75
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=204
PRIMER_PAIR_PENALTY_3=0.3178
PRIMER_LEFT_3_PENALTY=0.089133
PRIMER_RIGHT_3_PENALTY=0.228700
PRIMER_LEFT_3_SEQUENCE=AGAATTAGCATGTGCGAAGTGA
PRIMER_RIGHT_3_SEQUENCE=ACTTCCTANTTCCCGATTTTCC
PRIMER_LEFT_3=361,22
PRIMER_RIGHT_3=565,22
PRIMER_LEFT_3_TM=59.911
PRIMER_RIGHT_3_TM=59.771
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.75
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=0.3452
PRIMER_LEFT_4_PENALTY=0.116503
PRIMER_RIGHT_4_PENALTY=0.228700
PRIMER_LEFT_4_SEQUENCE=GCGTCTCCTTCAGAATTAGCAT
PRIMER_RIGHT_4_SEQUENCE=ACTTCCTANTTCCCGATTTTCC
PRIMER_LEFT_4=350,22
PRIMER_RIGHT_4=565,22
PRIMER_LEFT_4_TM=59.883
PRIMER_RIGHT_4_TM=59.771
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_03709 Ps_contig_mira-and-tgicl-ass_franssen_30805
SEQUENCE=AGGCATCGATCAGTCAGTATTTATAAATATTTAATATGNGTACGAAAATAATTCCTAAAAAATAAACTCTTTCACGTTACAATAGCCTTTACAACAATGCTTCGAACCAACTTCATATCATTATTAGGTTTCAAATAAATTGACACCAGATAGAACATACAATCAATGTCAAGGTAAACATGATTCATAACAATACTCAAACTAAGCTTCGTTCTTCCTCTTCTTCTGCTTAATACTTTAGAAAAGCATTTTTTGGCAATATGCAAGGCATTCGGATCCGTCCAGATAACGGATAAGAAAGAAGGATGCTTCATACAATCAGAGTTCATCATGATAATCATCCATGTAATCACGGCGATGCTTTCTATATCTGTCTCTATGTCTGTTTCGGTGTCGATCATAACCTCTATCTTTTCTCCGCCTTTCACCTTCTTCTCTTGCTCTTTCAGCCTCCTCCTCCTCTTGGGCTTTCCTTATTTTCTCTGCTTCCTCAAAACCCTCCTTTTCAATCTCTCTGTTGGCAAACACTTCATCAACAACTTGTTTTGCATAATCAGGATCATCACAAATCAAAATGTTGTCAAATACCGAACCACCTTTAACCTGCCAAACTTCAATGCCGACATACTTGATAGGCTTAAGCACGTAAAGATCGGGGTCATCTTCAAACTCTGGATTATCTAACCATGGAGTTTTCCATTTCCCTTTGTAGTTAGGATTCTTGATTTTCTTTCGTTTCCATGGTCCTTTGTATGCCGGATTTGGTATCTTAGGCCGTTTCCATATTCCATCTTCATCTTCATCCCAACTATCAGGCTCCTTAGCCTTCGGGTCAGGAATTTCTGCTGGGATTGAATCATATCCCTCCGGTTTGACCGAATTAGGGTCTTCAATATATTCTCTGTCGTCCCAGTCTGCAGGCTTTTTTGCCTTGACATCTTTGATTTTGCGAGGAGGAAGAATATCCCAATCTGTATACATGCTACCGGAATCACGCTCTCTATTATCAACCAGGACACTGTATGTAGCATCTGGCCTAAGGATGAATGTGTAGAAATGAGTTAACTTGTCCGTTTCACATTGCAGGTCCTTCTTTATCGGGTAATTTTGACCTTGGTAAGAGACTATGACATGGAGCTTCTTCGTGTCTGTGCCGCATAAATCTGGTCCAAACATTACGCTATAAGGCGTATCCCCACCGAACTTCTTTTGATTAACAAATCCAGAGAGGAGTTTCATGTAGCCTCCACCGCACTCAATCTCTTGTTCAAATTTTATAGAGTACTGAAAAACCAGTGTTCGGTTCTTGTTGGTGAATTCCGGTATCTTTGCAGAGATGGCAAAGTGTTTTGCATCATTCGATGTTTGGATACCTTTATCATCGGGATCTCCAGCCCATTTCCCAGCCGTGTGCTTAAACGAACCGGCTTTGCCTTCGCTTCTTTTCCAGTCTGATTTTACCCACCGACTTTGCCATCCATCTTCGAATCGTTCTTCAAAAATGATTTCTGAAAGGGAAACTTGTATTAGCAACAAAGAGAATAACACAAACATCTTGAGTTCAGTTGAAGCATTTTCCGCCATTTGCGGAATAAGCTATTTTTGCTCTGTCACCCCCGAAGTAC
TARGET=450,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0351
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=GTCTGTTTCGGTGTCGATCATA
PRIMER_RIGHT_SEQUENCE=TGAAGTTTGGCAGGTTAAAGGT
PRIMER_LEFT=380,22
PRIMER_RIGHT=615,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.0351
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=GTCTGTTTCGGTGTCGATCATA
PRIMER_RIGHT_1_SEQUENCE=GAAGTTTGGCAGGTTAAAGGTG
PRIMER_LEFT_1=380,22
PRIMER_RIGHT_1=614,22
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.1286
PRIMER_LEFT_2_PENALTY=0.000037
PRIMER_RIGHT_2_PENALTY=0.128591
PRIMER_LEFT_2_SEQUENCE=GTCTGTTTCGGTGTCGATCATA
PRIMER_RIGHT_2_SEQUENCE=GGTTTTGAGGAAGCAGAGAAAA
PRIMER_LEFT_2=380,22
PRIMER_RIGHT_2=497,22
PRIMER_LEFT_2_TM=60.000
PRIMER_RIGHT_2_TM=59.871
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=0.1511
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.151106
PRIMER_LEFT_3_SEQUENCE=GTCTGTTTCGGTGTCGATCATA
PRIMER_RIGHT_3_SEQUENCE=AGGTGGTTCGGTATTTGACAAC
PRIMER_LEFT_3=380,22
PRIMER_RIGHT_3=597,22
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.2199
PRIMER_LEFT_4_PENALTY=0.000037
PRIMER_RIGHT_4_PENALTY=0.219884
PRIMER_LEFT_4_SEQUENCE=GTCTGTTTCGGTGTCGATCATA
PRIMER_RIGHT_4_SEQUENCE=GTGTTTGCCAACAGAGAGATTG
PRIMER_LEFT_4=380,22
PRIMER_RIGHT_4=527,22
PRIMER_LEFT_4_TM=60.000
PRIMER_RIGHT_4_TM=59.780
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_03710 Ps_contig_mira-and-tgicl-ass_franssen_30811
SEQUENCE=ACTATAATAACATTGGTTTACGATATTAGTTTTTTTCGGTCACAATATCTTTTCCCTCTAACCATTCTTGTTACATACAAAACAACACTGCAATCTCTCTCTCGTTTTTCTTGTTCTTCAGATGGCTCTCAATTTGTCTTCTCCAACTGAATTCAAGTCTATTTTCTTCACTGATCGCCTCAAGTCTAACCGCTTCACACCTAAATTTCCAGGTGGTGTTGCTTTGAAAAGAGGAGATGATGGAAGAGCTTCTGGTGGAAGAAGAGTTTTTTGCTCAGTGCAGTCGTCATCACCACCACCAGCTTGGCCAGGACGAGCAGTTCCCGAACTAGGTTATAAGACTTGGGAAGGCCAAAAACCTATTTCCATTGTCGGATCTACCGGTTCAATTGGAACTCAGACATTAGATATCGTCGCGGAGAATCCAGATAAGTTTAAAGTTGTCGCGCTTGCAGCTGGTTCAAATGTTACTCTTCTTGTAGATCAGGTTAAAAGATTTAAGCCACAACTTGTTGCTGTTAGAAATGAGTCTCTAATTGCTGAACTTGAAGAGGCATTGAAAGATGTTGACCAAAAACCTGAGATCATCCCTGGAGAACAAGGAATCATTGAGGTTGCACGTCACCCAGATGCTGTAACCGTAGTCACAGGAATCGTAGGCTGTGCAGGACTAAAGCCAACAGTTGCGGCCATAGAAGCTGGAAAAGACATAGCTTTGGCCAATAAAGAGACACTCATTGCTGGAGGTCCTTTTGTTCTTCCTCTTGCTCGGAAGCATAATGTAAAAATCCTTCCTGCCGACTCCGAACATTCTGCCATTTTTCAGTGTATCCAGGGGTTGCCAGAGGGTGCACTTAGGCGAATTATTCTAACTGCATCTGGAGGTGCTTTTAGGGACTGGCCTGTTGAGAAACTGAAAGATGTTAAGGTAGCCGATGCATTAAAACATCCCAACTGGAGCATGGGGAAAAAGATAACTGTGGACTCTGCTACACTTTTTAACAAGGGTCTAGAAGTGATTGAAGCACATTACTTGTTTGGAGCTGATTATGATGATATTGATATTGTCATTCATCCACAATCAATCATACATTCAATGATTGAGACACAGGACTCGTCTGTTCTTGCACAATTGGGGTTGCCTGACATGCGTCTGCCAATCCTCTATACGTTATCATGGCCTGACAGAATTTATTGTTCTGAAGTCACTTGGCCTCGGCTCGATCTTAGCAAGATTGGTTCACTTACTTTTAAAGCACCTGACAACATTAAGTATCCATCGATGAATCTTGCCTATGCTGCTGGACGCGCTGGAGGCACCATGACAGGAGTTCTTAGTGCAGCAAATGAGAAAGCTGTAGAAATGTTTATCGATGAAAAGATAAGCTATTTGGATATTTTCAAGGTTGTGGAGCTAACATGTGAGAAGCATCAAGCTGAATTGTTAACCTCACCTTCCCTTGAAGAAATTATTCACTATGACTTGTGGGCGCGGGAATATGCGGCTAGTTTGCAAAGCTATACAAGATTCACTCCTGTTCCAGTATAAAACTGGTTTTAAATTTCTTTGAGCATTGAATAATAGTATAGAATGTAGCACAATGCATTCCCCAGATTATGAAAGATAATTTTTTATCAATGGAATCACTCAATATTCTTAGTCTCAATCTCTTCTTCAGCTGACATCTCATCAATTTGCCAGCACAAATATTATCCAAGTTCATGGTCATTAGTAGAAAAAGAGGTGATGTATTCAAACATGTTTTAAAAATGT
TARGET=94,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1654
PRIMER_LEFT_PENALTY=0.164639
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=CGGTCACAATATCTTTTCCCTC
PRIMER_RIGHT_SEQUENCE=CCTGGAAATTTAGGTGTGAAGC
PRIMER_LEFT=36,22
PRIMER_RIGHT=212,22
PRIMER_LEFT_TM=59.835
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.1654
PRIMER_LEFT_1_PENALTY=0.164639
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=TCGGTCACAATATCTTTTCCCT
PRIMER_RIGHT_1_SEQUENCE=CCTGGAAATTTAGGTGTGAAGC
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=212,22
PRIMER_LEFT_1_TM=59.835
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.1799
PRIMER_LEFT_2_PENALTY=0.164639
PRIMER_RIGHT_2_PENALTY=0.015253
PRIMER_LEFT_2_SEQUENCE=CGGTCACAATATCTTTTCCCTC
PRIMER_RIGHT_2_SEQUENCE=TAGACTTGAGGCGATCAGTGAA
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=187,22
PRIMER_LEFT_2_TM=59.835
PRIMER_RIGHT_2_TM=60.015
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.1846
PRIMER_LEFT_3_PENALTY=0.183834
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=TTCGGTCACAATATCTTTTCCC
PRIMER_RIGHT_3_SEQUENCE=CCTGGAAATTTAGGTGTGAAGC
PRIMER_LEFT_3=34,22
PRIMER_RIGHT_3=212,22
PRIMER_LEFT_3_TM=60.184
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=179
PRIMER_PAIR_PENALTY_4=0.1991
PRIMER_LEFT_4_PENALTY=0.183834
PRIMER_RIGHT_4_PENALTY=0.015253
PRIMER_LEFT_4_SEQUENCE=TTCGGTCACAATATCTTTTCCC
PRIMER_RIGHT_4_SEQUENCE=TAGACTTGAGGCGATCAGTGAA
PRIMER_LEFT_4=34,22
PRIMER_RIGHT_4=187,22
PRIMER_LEFT_4_TM=60.184
PRIMER_RIGHT_4_TM=60.015
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_03711 Ps_contig_mira-and-tgicl-ass_franssen_30816
SEQUENCE=AAAGAACAAAGATAGTGAAATCCAAAACCAAAGTTTAAGTACATAAATCATGATATTAAACTACTCAGACCAGTTTCTCAGTTTACATATGCCTCATCAACTATGTAGCCACAAGATACAAACTATAAATACATACATAATAAGTAGTTAGTTAGAACTTAGAATGCAAGAAATGTCTTATTAGTTGAGCTTCCCCTTTATGAGTTTAGCAGCTTGGATCTGTTCTGCAAGTGATACTTTTTTCTGCATTTTCTCTCTTATGATAAGCTCTAAATCCAATTTACCGTGAGTTGCAAAATTTGAATTCATAATGTCTCCTTTTTCAGCTACTTCATTGCTGATGTTGTTACTAACATGATGTTCAGTTAATTTAGAGCCAAGTTTGAATTTGTAAGTAACTTCCAAGGCATGTTTGTCTTGCGCTGCATTTTTGTATGGTTGACTCTTTTCTTTCTCTCTGGCTTTAGTCCTCTCTCGGAGATCTCTTTTCTTTTGAGTAAGCATTACCTTTTTTATTGCTTTTATCTGTGCTTCTTTATCAGCTTTTATTTTTGCAATCTCTTTGGCTTTTTCCTTCTCTGATTTACGTCGAAGCTGATAAAGGAGAAGCAATTCTTCTTTTATCTTTTCGTAGCTATCCCAATCTAGTTCTTTTTCTTCACTCCCTCCTTCCTTGGCTGCTTTGGCTATGTTTTCCATCCAAGACTGAATGAATTTCTCTGCTCTAGCAATTGCCATCAGCTTTTCTCTTTCCTTTCCCTCTAATTGCGAAATTCGGTGGAGCTCCAGTTGTTCTTTTATCTTATCATAGCTGTCCCAATCTAACTCTTCCTGACCACTCCCTCCTTTCATGGCTGCATTTGCTATGTTTTGTTCCCATAAGAGAAAAAATAGCTCCCGCACAAGCTTTGACTTCTGCCGCTCTTTCCATGCACGTCTCTGTGAATAACTTATCTTTGCCTTGATTTGATTGCTATGATGAGAACGTGCATGTTCAGCCATCTTCTTCTTCACCTTGGGGTCTTTCAAAGCTTCTATCGTTCTAATCCTGATGAGTTCACGGGTCTCTGCGGTGTGTTTCCTTCCCTTATTCCAAGGTGCTTTTCCTTTGTTAGCTAGTCCTATTCTCATTCTTCTCGTCATCTCTTTTTCATCCACAAATTGATTATCTTTCTTTGAATCTTGTTCGACGTTGTGGCTTCCATTATCAACAACAGTAGGGAGAACAGAAGAAGAAGAAGCAGAGAATGTTAATTTGGGGAGGTGGCGGAGTGTTATTGGAGGGGGGAATGTAGAAGCGTAATAAACTCTATTTGTGAAGAGTGAATAATGATTGTAGGGAATGTGAGTGAAGGGAGTGTGTAGAGCTGACATTGAGATGAACATGAATCCCCCGAAC
TARGET=1228,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0278
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.024206
PRIMER_LEFT_SEQUENCE=TGGCTTCCATTATCAACAACAG
PRIMER_RIGHT_SEQUENCE=GGGGATTCATGTTCATCTCAAT
PRIMER_LEFT=1195,22
PRIMER_RIGHT=1393,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.024
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.0544
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.050763
PRIMER_LEFT_1_SEQUENCE=TGGCTTCCATTATCAACAACAG
PRIMER_RIGHT_1_SEQUENCE=TCAGCTCTACACACTCCCTTCA
PRIMER_LEFT_1=1195,22
PRIMER_RIGHT_1=1370,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=60.051
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=0.1346
PRIMER_LEFT_2_PENALTY=0.110392
PRIMER_RIGHT_2_PENALTY=0.024206
PRIMER_LEFT_2_SEQUENCE=CGTCATCTCTTTTTCATCCACA
PRIMER_RIGHT_2_SEQUENCE=GGGGATTCATGTTCATCTCAAT
PRIMER_LEFT_2=1137,22
PRIMER_RIGHT_2=1393,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.024
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.1571
PRIMER_LEFT_3_PENALTY=0.132857
PRIMER_RIGHT_3_PENALTY=0.024206
PRIMER_LEFT_3_SEQUENCE=GTTGTGGCTTCCATTATCAACA
PRIMER_RIGHT_3_SEQUENCE=GGGGATTCATGTTCATCTCAAT
PRIMER_LEFT_3=1191,22
PRIMER_RIGHT_3=1393,22
PRIMER_LEFT_3_TM=59.867
PRIMER_RIGHT_3_TM=60.024
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.1571
PRIMER_LEFT_4_PENALTY=0.132857
PRIMER_RIGHT_4_PENALTY=0.024206
PRIMER_LEFT_4_SEQUENCE=GTGGCTTCCATTATCAACAACA
PRIMER_RIGHT_4_SEQUENCE=GGGGATTCATGTTCATCTCAAT
PRIMER_LEFT_4=1194,22
PRIMER_RIGHT_4=1393,22
PRIMER_LEFT_4_TM=59.867
PRIMER_RIGHT_4_TM=60.024
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_03712 Ps_contig_mira-and-tgicl-ass_franssen_30817
SEQUENCE=GTGGTAGTCAACGCAGAGTAGYNGGGGAAACCAAGTTCTTCTACTCCCTCTTTACTTTCAAGTTCCAATAGCTAAGAAATAAAATGGGTTCCAAACTTCCATTACTATTTGTTTTTGTGATGTTGTTTGCTTTAAGTTCAGCAATTCCAAACAAGAGAAAGCCATATAAACCATGCAAAAACCTAGTCTTTTATTTTCATGACATACTTTACAATGGAAAGAATGCAGCAAATGCAACATCAGCAATAGTAGCAGCTCCAGAAGGTGTTAGTTTAACTAAATTGGCACCTCAATCCCACTTTGGTAACATAATAGTTTTTGATGACCCTATCACATTAAGCCATAGCCTTTCTTCAAAACAAGTTGGAAGAGCACAAGGGTTTTATATTTATGATACCAAAAACACATACACTTCTTGGCTTAGTTTCACTTTTGTTCTTAATAGCACTCATCATCAAGGAACCATTACTTTTGCTGGAGCTGACCCAATTGTCGCCAAAACTAGAGATATTTCTGTCACTGGTGGTACTGGAGATTTCTTTATGCATAGAGGAATTGCTACTATTACCACTGATGCCTTTGAAGGCGAGGCTTATTTTCGACTTGGTGTTTATATCAAGTTCTTTGAGTGTTGGTAACTATCAAATTAAGTACTACTTACTATAATAAAAGCAATTAAATTTGAAGTTAAATTATTGTTGTCTCTTTTCATGTTGTGTTTTTAATTAATTAGTCCATAAAGTATACTCTTTATATTTCTTTTGTTCTCTATTATTATTATCAATAAATGAAAATCCTATCNACTAGTTTCTTT
TARGET=770,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03713 Ps_contig_mira-and-tgicl-ass_franssen_30822
SEQUENCE=CTNCTCACCTAAAACGCGCTTTTCTTTATTCCTTCACCACTACTCAAATACCTATATATACACATATTCTATCAGTTNGGCGATCATCCAATTTACTCACACACACACTTTTGACTTTCCTTTATACAAAAAATCAACAGCAATGAATCACAATATCAATCAACAACAATCTCCTGTAATGGCATATCCAGCAATGGGTGAGAAGAACCACCAAGTTGCTCCACCACCACCAATGGGTTACCCTACAAAGGATGATCCCCAACAAACTGTTCCAGTCAGTACAACTACCAGGGGTGATGGTTTCTGGAAAGGATGTTGTGCTGGATTATGTTGTTGCTGTGCCTTGGATTGCTGCCTCTGATTGAATATCATACTAGTATTTTTGTAACTTGTTTAATAAGGATCTTTGTATCATATATGGGATAGCAAAAACTCATTCTATCGATTTCTAAGTTATGGATTTTTAATTTTCTTTGTATTCTGTCGTTGCAAAATTCAGAACCATATAGTGAATTAATTTG
TARGET=98,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6823
PRIMER_LEFT_PENALTY=0.580205
PRIMER_RIGHT_PENALTY=0.102139
PRIMER_LEFT_SEQUENCE=GCGCTTTTCTTTATTCCTTCAC
PRIMER_RIGHT_SEQUENCE=TCCTTTGTAGGGTAACCCATTG
PRIMER_LEFT=15,22
PRIMER_RIGHT=251,22
PRIMER_LEFT_TM=59.420
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.8124
PRIMER_LEFT_1_PENALTY=0.710267
PRIMER_RIGHT_1_PENALTY=0.102139
PRIMER_LEFT_1_SEQUENCE=CGCTTTTCTTTATTCCTTCACC
PRIMER_RIGHT_1_SEQUENCE=TCCTTTGTAGGGTAACCCATTG
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=251,22
PRIMER_LEFT_1_TM=59.290
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.8691
PRIMER_LEFT_2_PENALTY=0.580205
PRIMER_RIGHT_2_PENALTY=0.288917
PRIMER_LEFT_2_SEQUENCE=GCGCTTTTCTTTATTCCTTCAC
PRIMER_RIGHT_2_SEQUENCE=GAGCAACTTGGTGGTTCTTCTC
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=220,22
PRIMER_LEFT_2_TM=59.420
PRIMER_RIGHT_2_TM=60.289
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.8765
PRIMER_LEFT_3_PENALTY=0.816062
PRIMER_RIGHT_3_PENALTY=0.060408
PRIMER_LEFT_3_SEQUENCE=GTTNGGCGATCATCCAATTTAC
PRIMER_RIGHT_3_SEQUENCE=CAAGGCACAGCAACAACATAAT
PRIMER_LEFT_3=74,22
PRIMER_RIGHT_3=345,22
PRIMER_LEFT_3_TM=60.816
PRIMER_RIGHT_3_TM=60.060
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.50
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=5.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=272
PRIMER_PAIR_PENALTY_4=0.9182
PRIMER_LEFT_4_PENALTY=0.816062
PRIMER_RIGHT_4_PENALTY=0.102139
PRIMER_LEFT_4_SEQUENCE=GTTNGGCGATCATCCAATTTAC
PRIMER_RIGHT_4_SEQUENCE=TCCTTTGTAGGGTAACCCATTG
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=251,22
PRIMER_LEFT_4_TM=60.816
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.50
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_03714 Ps_contig_mira-and-tgicl-ass_franssen_30822
SEQUENCE=CTNCTCACCTAAAACGCGCTTTTCTTTATTCCTTCACCACTACTCAAATACCTATATATACACATATTCTATCAGTTNGGCGATCATCCAATTTACTCACACACACACTTTTGACTTTCCTTTATACAAAAAATCAACAGCAATGAATCACAATATCAATCAACAACAATCTCCTGTAATGGCATATCCAGCAATGGGTGAGAAGAACCACCAAGTTGCTCCACCACCACCAATGGGTTACCCTACAAAGGATGATCCCCAACAAACTGTTCCAGTCAGTACAACTACCAGGGGTGATGGTTTCTGGAAAGGATGTTGTGCTGGATTATGTTGTTGCTGTGCCTTGGATTGCTGCCTCTGATTGAATATCATACTAGTATTTTTGTAACTTGTTTAATAAGGATCTTTGTATCATATATGGGATAGCAAAAACTCATTCTATCGATTTCTAAGTTATGGATTTTTAATTTTCTTTGTATTCTGTCGTTGCAAAATTCAGAACCATATAGTGAATTAATTTG
TARGET=221,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0939
PRIMER_LEFT_PENALTY=0.033470
PRIMER_RIGHT_PENALTY=0.060408
PRIMER_LEFT_SEQUENCE=GCGATCATCCAATTTACTCACA
PRIMER_RIGHT_SEQUENCE=CAAGGCACAGCAACAACATAAT
PRIMER_LEFT=79,22
PRIMER_RIGHT=345,22
PRIMER_LEFT_TM=59.967
PRIMER_RIGHT_TM=60.060
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.3493
PRIMER_LEFT_1_PENALTY=0.288917
PRIMER_RIGHT_1_PENALTY=0.060408
PRIMER_LEFT_1_SEQUENCE=GAGAAGAACCACCAAGTTGCTC
PRIMER_RIGHT_1_SEQUENCE=CAAGGCACAGCAACAACATAAT
PRIMER_LEFT_1=199,22
PRIMER_RIGHT_1=345,22
PRIMER_LEFT_1_TM=60.289
PRIMER_RIGHT_1_TM=60.060
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.4004
PRIMER_LEFT_2_PENALTY=0.340007
PRIMER_RIGHT_2_PENALTY=0.060408
PRIMER_LEFT_2_SEQUENCE=ATATCCAGCAATGGGTGAGAAG
PRIMER_RIGHT_2_SEQUENCE=CAAGGCACAGCAACAACATAAT
PRIMER_LEFT_2=183,22
PRIMER_RIGHT_2=345,22
PRIMER_LEFT_2_TM=60.340
PRIMER_RIGHT_2_TM=60.060
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=0.5187
PRIMER_LEFT_3_PENALTY=0.033470
PRIMER_RIGHT_3_PENALTY=0.485189
PRIMER_LEFT_3_SEQUENCE=GCGATCATCCAATTTACTCACA
PRIMER_RIGHT_3_SEQUENCE=TCACCCCTGGTAGTTGTACTGA
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=59.967
PRIMER_RIGHT_3_TM=59.515
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.5616
PRIMER_LEFT_4_PENALTY=0.501224
PRIMER_RIGHT_4_PENALTY=0.060408
PRIMER_LEFT_4_SEQUENCE=ACAACAATCTCCTGTAATGGCA
PRIMER_RIGHT_4_SEQUENCE=CAAGGCACAGCAACAACATAAT
PRIMER_LEFT_4=162,22
PRIMER_RIGHT_4=345,22
PRIMER_LEFT_4_TM=59.499
PRIMER_RIGHT_4_TM=60.060
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=5.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_03715 Ps_contig_mira-and-tgicl-ass_franssen_30825
SEQUENCE=ATTAAGTCAGGGAAAGGGCCAAAGNAATTCAAAATGGGAACACAGAATTATGTGCCGGCATGGAGAAATTTATAGTCAATTTCGACGTCCACACTTTTAGGGCCATTAACAAAATGAAATCAGCACTCTCTTAAGTTTGAGCAAACATGCTCTTAATCATGCATTTTAACCCTCTTGGTAACCAAGTTTAGCAATTGAAAGATAGTTCATAATGTGAAACAAAATAGCAATATATATGCATACATAAATTTGTGTAATCCATGAAACTAATGCTACACCTACAATACTCGTTGGATGGGAGATTTGTGCTGTGCCGGACAAACACTCAAGCAAGTTCCTGGGAAATTTGGGAATGGGAATGGTGAACAACCAGGGAACCTTCTTGCAAAATAGACTTTAAATTACAGCTGCTAATAGGTTTTTGTTTTGGGAACCTTCTTCTTGGGTTGCCTTCTAGGAATGGAGTTTTTCATCCCAATGAAAAACAATAAAGCCCCGAAGAGAGCCATATTCTGAGTAAATTTGATGAAAAGTTGAATATATTCTTTGTCCTCATGGTCGTAATTATAGAAATCATACAATATTGGAGTAGTAATCACCTGGTGCAAAAGCAGAAGTGTAGCTCCAAGCGAACTGCCAAGTATGAAAAAAATTCCTCCAAAGCCCTTGAGAGCAATAGCCCCAGCAACTAAAAATTTTATATCAATTTCTGGAAGTTGGAAGCCAGCATGTGAATCAACTTTATTTATAAAGGCATCAAACTTTGGTCTGATTGCTTTTGCTGCAGGTCCCCCGTCAACTCCATAGTCATTAAATTCTTGGTAAGCAGAGAGGATGAAGACGGAGGCGAAGAGGACTCTTCCGAGGAAAGAAGCGAACGCCATTGTGGATATTGTAGAGAGAGAGAGAAGAGAGAGAAAGTAGGGTTGAGTGGAATTTAGTGTTGTGTTGTTTTTCCCAGTCAGAGGCAGAACAAAGACTAAAGAGAAAAGAGAGCGCCAGCGAAAATTGAAAATGAGAGAGA
TARGET=897,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1832
PRIMER_LEFT_PENALTY=0.138114
PRIMER_RIGHT_PENALTY=0.045112
PRIMER_LEFT_SEQUENCE=GCCAGCATGTGAATCAACTTTA
PRIMER_RIGHT_SEQUENCE=CTGACTGGGAAAAACAACACAA
PRIMER_LEFT=722,22
PRIMER_RIGHT=964,22
PRIMER_LEFT_TM=60.138
PRIMER_RIGHT_TM=60.045
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.2245
PRIMER_LEFT_1_PENALTY=0.138114
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=GCCAGCATGTGAATCAACTTTA
PRIMER_RIGHT_1_SEQUENCE=GACTGGGAAAAACAACACAACA
PRIMER_LEFT_1=722,22
PRIMER_RIGHT_1=962,22
PRIMER_LEFT_1_TM=60.138
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.2245
PRIMER_LEFT_2_PENALTY=0.138114
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=GCCAGCATGTGAATCAACTTTA
PRIMER_RIGHT_2_SEQUENCE=TGACTGGGAAAAACAACACAAC
PRIMER_LEFT_2=722,22
PRIMER_RIGHT_2=963,22
PRIMER_LEFT_2_TM=60.138
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.4070
PRIMER_LEFT_3_PENALTY=0.138114
PRIMER_RIGHT_3_PENALTY=0.268895
PRIMER_LEFT_3_SEQUENCE=GCCAGCATGTGAATCAACTTTA
PRIMER_RIGHT_3_SEQUENCE=TGGCGCTCTCTTTTCTCTTTAG
PRIMER_LEFT_3=722,22
PRIMER_RIGHT_3=1000,22
PRIMER_LEFT_3_TM=60.138
PRIMER_RIGHT_3_TM=60.269
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.4070
PRIMER_LEFT_4_PENALTY=0.138114
PRIMER_RIGHT_4_PENALTY=0.268895
PRIMER_LEFT_4_SEQUENCE=GCCAGCATGTGAATCAACTTTA
PRIMER_RIGHT_4_SEQUENCE=CTGGCGCTCTCTTTTCTCTTTA
PRIMER_LEFT_4=722,22
PRIMER_RIGHT_4=1001,22
PRIMER_LEFT_4_TM=60.138
PRIMER_RIGHT_4_TM=60.269
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_03716 Ps_contig_mira-and-tgicl-ass_franssen_30830
SEQUENCE=TAACGGAAGTAAAGAGTAGCAGAAAGAGTGAGTTCGAACAATGGAGACTCCTCCGTCGCAGCAAGGCGTAGTAAAGAAGAAGGAAACTCGAGGTCGAAAACCTAAACCAAAAGACGATCAACAACAAACACCTGCCAAAACACTGAAAGAAGCGAAAAAGGCGCAACTACAACAGCAGCAACAACAGCAACTGCAGCAACAGCAGCAACAACAACAACACCAAGCTTCGGTAGACGAGAAATACACTCAATGGAAGTCTCTCGTTCCCGTTCTCTACGATTGGCTTGCGAATCATAACCTTGTTTGGCCTTCTCTCTCTTGCAGGTGGGGTCCTCAGCTTGAACAAGCTACTTATAAGAATCGGCAGCGGCTTTATCTTTCTGAGCAGACTGATGGTAGTGTCCCAAATACTTTGGTGATTGCGAATTGTGAGGTTGTGAAAACTAGGGTTGCAGCTGCAGAGCATATTTCACAGTTTAATGAGGAGGCTCGCTCTCCATTTGTTAAGAAGTACAAGACCATCATACATCCCGGGGAGGTGAACAGAATTAGGGAATTGCCGCAAAATTCTAAGATAGTGGCTACTCACACAGACAGCCCTGATGTTCTCATTTGGGATGTTGAAAGTCAACCTAACCGTCACGCTGTCCTTGGAGCCACAAACTCTCGTCCAGATTTGATATTGACCGGACACCAAGACAATGCTGAGTTTGCTCTTGCGATGTGCCCAACTGAGCCTTATGTCCTTTCAGGAGGAAAAGATAAAACAGTGGTGTTGTGGAGTATTGAAGACCATGTAACATCTGCTGCTACGGACAAGTCTGGTGGATCCATTATCAAACCGAACTCTAAATCTGGGGAAGGCAATGACAAAACTGTTGATAGCCCTTCTGTCGGGCCAAGAGGTATCTACTCTGGGCACGATGATACTGTTGAAGATGTGGCCTTTTGCCCTTCTAGTGCGCAGGAATTCTGTAGTGTTGGAGATGATTCTTGTCTCATATTATGGGATGCACGTGTTGGCTCTAGTCCTGTTGTTAAGGTTGAAAAAGCTCATAATGCCGATCTTCACTGTGTTGACTGGAATCCCCATGATGATAATCTGATTCTTACTGGGTCGGCAGATAATTCTGTTCGCTTGTTTGATCGGCGCAATCTCACCTCTAATGGGGTTGGGTCTCCTATTCATAAATTTGAGGCTCATAAAGCTGCCGTTCTTTGTGTTCAGTGGTCTCCAGACAAATCATCTGTATTTGGAAGTTCAGCAGAAGACGGTCTCTTAAACATTTGGGATTATGAGAAGGTTGGTAAAAAGATTGAGCGAGCTGGAAAAACAATAAACTCTCCTCCAGGGTTGTTTTTCCAACATGCTGGTCATAGAGACAAAGTTGTTGACTTCCATTGGAATGCTTATGATCCATGGACAATTGTAAGTGTGTCTGATGATTGTGAAAGTACTGGTGGAGGAGGAACATTGCAGATATGGCGCATGAGTGATTTGCTCTATAGACCAGAGGATGAGGTTTTGGCAGAGTTGGAGAAATTCAAATCTCACGTGGTGGCTTGTGCTGCAAAGACCGATACATAATTTACTTCGTAGACGAAGGCTTGTGTAATTTATCTGTTTTATTTACTTTTGTTATGGATTCATCATTGCCATTTTAGAGAACAATCACATGTATGTGGCTCCATTTTCTCTAGACAGAACTAGATGTAGGATGGAGATTGGAGAATGAATAGGCTCATTTACCCTAAAATGTTTCTATATTATTTTTTTGTGACTTAAATTCTTAAGGNYAANTTTTACGTTGGGTAGCATTACCTTTAATACTAAAATTTAT
TARGET=206,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03717 Ps_contig_mira-and-tgicl-ass_franssen_30833
SEQUENCE=CGTCGGGGGTAAGTCGTTTTGTACTGTAAGTGGAACTAACATAAACGATTACGCAATAATCAACCAGAAACGGAACAAGAACGAAGAAGAAGAAGAATCGAGGCAATAATCATGGCAGGTCGATTAACAAACGTAGCATCACAAATCTTAGGCGGAAACGGCGTCGTTCACCGATCGGTAGCTTCCTCGCTTCGCCTCCGCTCCGGCATGGGTCTCCCCGTCGGCAAACACTATGTCCCAAACAAACCCCTTCCCGTGAATGAGGAACTTACATGGGACAACGGTACTGCATATCCCGAACCTTGTATAGATCGCATTGCTGATACTGTTGGAAAGTATGAAGCTTTGGCTTGGCTATGTGGAGGGTTGAGCTGTTTTGGAGCTCTGGGATTATTGGCTGTGTGGAATGATAAAGCCTCTAAGACACCATTTGCACCCAAAGTATATCCATATGACAATCTGCGAGTGGAGCTTGGTGGTGAGCCATAAGTAGATCCAAGGCAATAATGGGATGATTACTTCTTTTATGTATTTGTTCTGTTCATTTGTACTCCTATCTTATCTTATGTGGTCGAAAATAATGGATCAGAAAGACTTGCTGAAACCAAATACAGGAATGAAAGAGCTGAATCTGGTTTAATTCTTTTGATCATTTTCTCATGTTTGTGATGGCAATAGTGAGTTGGCAAAGTTTATTCTCCATAATTTCTCCACTCTTTATGGAACTGATATATCTTACCAAAGAAATTAAAAAATTTGTTAA
TARGET=83,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2968
PRIMER_LEFT_PENALTY=0.201297
PRIMER_RIGHT_PENALTY=0.095538
PRIMER_LEFT_SEQUENCE=GGGGGTAAGTCGTTTTGTACTG
PRIMER_RIGHT_SEQUENCE=TTGTTTGGGACATAGTGTTTGC
PRIMER_LEFT=4,22
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=59.799
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=0.4428
PRIMER_LEFT_1_PENALTY=0.201297
PRIMER_RIGHT_1_PENALTY=0.241544
PRIMER_LEFT_1_SEQUENCE=GGGGGTAAGTCGTTTTGTACTG
PRIMER_RIGHT_1_SEQUENCE=ATGTAAGTTCCTCATTCACGGG
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=273,22
PRIMER_LEFT_1_TM=59.799
PRIMER_RIGHT_1_TM=60.242
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=11.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.4802
PRIMER_LEFT_2_PENALTY=0.384647
PRIMER_RIGHT_2_PENALTY=0.095538
PRIMER_LEFT_2_SEQUENCE=AATCAACCAGAAACGGAACAAG
PRIMER_RIGHT_2_SEQUENCE=TTGTTTGGGACATAGTGTTTGC
PRIMER_LEFT_2=57,22
PRIMER_RIGHT_2=244,22
PRIMER_LEFT_2_TM=60.385
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.5799
PRIMER_LEFT_3_PENALTY=0.484403
PRIMER_RIGHT_3_PENALTY=0.095538
PRIMER_LEFT_3_SEQUENCE=CGATTACGCAATAATCAACCAG
PRIMER_RIGHT_3_SEQUENCE=TTGTTTGGGACATAGTGTTTGC
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=244,22
PRIMER_LEFT_3_TM=59.516
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=0.6210
PRIMER_LEFT_4_PENALTY=0.384647
PRIMER_RIGHT_4_PENALTY=0.236361
PRIMER_LEFT_4_SEQUENCE=AATCAACCAGAAACGGAACAAG
PRIMER_RIGHT_4_SEQUENCE=CAGCAATGCGATCTATACAAGG
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=321,22
PRIMER_LEFT_4_TM=60.385
PRIMER_RIGHT_4_TM=59.764
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_03718 Ps_contig_mira-and-tgicl-ass_franssen_30839
SEQUENCE=GTTTGAGTGAAATCCAGCATTGACACCTTNTTCCTTTCAAGGGACTCAAAATTTGCATTCCAAGGGATATATATTTAATCAACAAGTTCTGTGTGGCAAATTGCATCCATCATACACAGTTGGAGCTCTCCATATAACAGTGATAACAAAGTACTGTGTAGTACATACAAACCACTGAAAACAATAGGAATTTAAGTGAGGCGGGTAAGTACAGCAGAATCACTTATGAACAACATGATGCAGCCCTAGATCCTTCAGCGGGTAAGTACAGCAGAATCACTTATGAACAACATGATGCAGCACTAGATCCTTCGGCGGGTCTATCAACAAGAACCATCCCTGCTGGACTTTGAGCTGGCTGAGCCCTTGGTAATCTCTTGGCAATTTCATAAAATACTTCATTAACATTGGCCGCAGATTTGGCAGAGGTCTCCATGAAAAACAGACCATTTTCCTCTGCATATACACGTGCTTCTTCGGCTGTCACTTTTCTTTTGTCTTCCAAGTCAGCTTTGTTGCCGGTAAGAGCCATGACCATGTTCGGATTACCTTGCTTTTGAAGCTCTTGGACCCACTTTTTAGCTCGTGAAAATGAATCAGAGCTAGTTATGTCATAGACAATGATAGCAGCAGCAGCGCCTCTGTAATACATGGGAGCCAAGCTATGGTATCTCTCCTGTCCTGCCGTGTCCCAAATCTCGAATTTCACCGTTGCATCATTTACTGCCAATGTCTGTGAAAAGAATGCCGCTCCTATTGTTGATTCCTGAAATTCAAGAAATTGACCCTTGACAAATCGCAAAACGAGGCTGGATTTACCGGCACCCATATCCCCAAGAAGAACCAATTTTGCATTGAGATTGTTGTGTCCGATCGTAGCCATAGGAAGATAATTGGATCGATCGGAAGCAAACAGAGAAACGAGAACCGAAAACAAGAAAAATCAGAACTTCGAATTTAGCCGATACGAAGAATTCAATATTTGAAAATGATCGAATCGAAGAGACAGAGAAAGAAGAGTCTCTGTCTGAATTCTATTGCTTTTCGATTACGGTGAGTTTCGTAGCTATAGCGTCTAGCGTCTGCGTTTAAACGCTTTCGTTTTGTTTGTTTTTCNYCGNYTTGTCTT
TARGET=626,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03719 Ps_contig_mira-and-tgicl-ass_franssen_30842
SEQUENCE=TCGGGGATACAAAAAAGTGAGTACTCCATTTTAGANGAAAACCAGTGAGTCCCATCACAGCCCTCACTCAGGTCAGCCATGGATCGCGCTCAGCTTCTGTTGGTTGGTTTACCTCTCTTCCTTCTCTGTTCCGACCTCTTTTCCCTTTTCACATCATCTCCTCCGCCGCCCAAGCCATCGCACCACCACCACCACCACCATCAACAACCACATCCAGTTGTTGACCCCGTAGCACCGTTTCCCTCCCACTCCGAGAGACTCACCTCAAATATTGGAGATGTAGGTTACGGCAACACTGTCAACATCAATTTTTGCTCTTCTTGCTCCTACAAGGGAACAGCGGTGACACTAAAGAATATGTTGGAGATTGCTTTTCCTGGAGTTGAGGTTATTCTTGCAAACTATCCTCCACCACTTCCAAAACGTCTGCTAAGTAAAGTGGTTCCAGTTGTCCAAATTGGGGTTGTAGGTGTTGTAGTTGCAGGCGAACAGATTTTTCCAATGTTGGGTTTTGTTGCACCTCCTCCTTGGTATTTTAACTTGCGTGCTAATAGGTTTGGAACCATTGCTACTACTTGGCTTCTGGGGAATGCCTTGCAGTCATTCTTGCAAAGCTCTGGGGCATTTGAAGTTTACTTCAACGGCAATCTGGTGTTCTCTAAACTGAAAGAGGGAAGGTTTCCCGGAGAGATAGAGTTGAAGGATCTGATCACCAGAAGGATGGCAAATTCTAAACTTGTCAGCAGTGCTTCAGAATTGGGTCTTTAGCTCATTAGTCTAATTGCCCCTCACGAAGACATCTAATTTGAACAGGGGAAGATCATGTAAATGTACCTACCATTTGCATTGTGAAGAAGAGGCTGATTCACGGATATCATAGGAATGTATTGCTGCAAATGGACTGTGTTGAAAGAACCAAGTAACTTCTAAAGACAAACTTGGTTAAATTAGATTTGTCATATACATAGCTTATAGCCTTACACTATCATTTTACCTTCTGGCTTTGCTACATTATGATATTGCGCTATTGTCGGCTCATGTAAATTCCATCTCCTACCTAAAAARGTTAA
TARGET=181,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03720 Ps_contig_mira-and-tgicl-ass_franssen_30844
SEQUENCE=CATACCTCGGAGCAACATCCATATTTGTATTTCTACCGTCTAAACCCTAGTCTTCTACCAAGCGCCAAAAAACCAAGAGCCACTGAAGAACAAAACACAGCACCACTTGAAGTTCTGAAACTCGGTATCACGACCAACGCAAACGAAGCAGCTAAAAACCCAACTGTATTCAGAAACTTCCTCGTCCCAGTCACAGACATTATCCTCCTTGTAATCAAATAATCAGCAACAACACCACCAATATTCGAGAACACAAACATATTAAGATAAGGCAACATTTTCGACGAACCCATTTCATGAAGACTAAGCTTAAGCCCCAATTCAAAATAAGTCGGTAACCAGTTCATCAACATATACAAAGCATAATGAAAAGTAAAATTATTAACCACAATAGCCCAAACAGGTAAACTAGTCATAATCTTCATCCAAGGAATTCCAACTCCATTTTTCTTAACACCATTTCTCTCAACTCCAGTTTCCACATTTACATCCGAAACTTTCCTATCCATTTTCTTATTAACCGGAAAAAGCAATCCCCTGGCTCCCGAAGCCGAAGCTTTAGGATCAGAAGCATATCTAAACCAAAGCAAAGACCAAGCAAATCCTAAAAACGCTTCCGCGATAAAAACAGATTGAGGACCTTTAAACTTAACCAAACTCGGAAGAAACAACATTCCAACAGAAGCACCAAGATACATACCAGAAGCTTGAACGCCTGGTCTTTGTACTCTAATAGCAACAACCTGCCCTGTTCTGCGAAGCCTAGTTTGATAAACCTGCCCGAGTGAAGCAGCAACAACAGGCTTGGGTGAAATCCCTGAGAAAATATCAGCCAAGAATAAACCAAGTTCTTGTTCAATCATGTTGAAAGCAACTTCAGTGGAGAATGGACTTATTCGGTCTTGCAGTAGTGAGGGCTCATCTAGACATGATGGTGGTATCAAATCAGAACGAGATGATATAGCCTAAACAATTTTGGTATATGCCGGCCCAAGCTCCACCAATATCTTCCTTAGCCCTGCAGCTCTAACCTGAAACACATCATCAGAGAGATCAAATCGAGCGTCCAGATAGCGTAAACCAAACCACTTGCCAAAAGCAGTTCCAGTCTGAAACAATCTTCTAGCAACAACAAGCGGCTTCCTGAAGTAAATAGCCGCAATTTTCGATATACTATAATCGGCCAGAGAATCAACTTCCGTCGCGCTGAGTTCAAACAAGTAACCTGACTTCGCCGTAAAGGAGTCACCGCTATCGGAGCCAATCGAGACGGCGGTGCATGTGACGGAGCAACGACGACGACGAAGTTCCAATCGATAAACATAGTTCCGGCGGTGGTCGTGTTGGTACGACGAGGGTTTTGTTGTTGGTGGTTGCGGGAATAGATGGAAGTTATAGATCCGACGTCGTTCGTTGAGTAGATCGATGGTGGAAGGTTGTGTTTGGGGTGGTGAAGACGGATGGTTTTGACGGTAGATGGTGGGTTTGCGGCGGCTGAGGGTTTGTAAGGTTGTTGTTGTTTTATTTTTGTGTGTTTAGAAGACGAACGAAAAAAGGGAGGTCGCCAGCAGAGTGAGGTAGAGCGGAGACAGTTGAGGATGATGGTTTTGATGGAGGAAAGGGAGCGGTTGAGTGGGGGAAGAGGATGAAGGAGTCCCGTCTTGGGTGGCGGTCGGAGGAATACAACGGAGGGTATGATGAGTAGAGAGTGGATGTTGCGGAGATGAAGGTGAAGGGTTTGTGGAGGAGGGAGGCGGATATGCGGTGGTTTTGTGGAGTGAAGAAGAGGAAAGGTGGTTTTTTTGTTTTATCTTTTCAGAGGAGCGAGAGAGAAGAGAGAGGGTCAACAATTGAGAGTGGTGGGTGGGTAGGTATAGGGGGGACGCGTGTGAGGAGGCGCCGCTTAGGATTCACCATGTCTCYC
TARGET=1293,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03721 Ps_contig_mira-and-tgicl-ass_franssen_30844
SEQUENCE=CATACCTCGGAGCAACATCCATATTTGTATTTCTACCGTCTAAACCCTAGTCTTCTACCAAGCGCCAAAAAACCAAGAGCCACTGAAGAACAAAACACAGCACCACTTGAAGTTCTGAAACTCGGTATCACGACCAACGCAAACGAAGCAGCTAAAAACCCAACTGTATTCAGAAACTTCCTCGTCCCAGTCACAGACATTATCCTCCTTGTAATCAAATAATCAGCAACAACACCACCAATATTCGAGAACACAAACATATTAAGATAAGGCAACATTTTCGACGAACCCATTTCATGAAGACTAAGCTTAAGCCCCAATTCAAAATAAGTCGGTAACCAGTTCATCAACATATACAAAGCATAATGAAAAGTAAAATTATTAACCACAATAGCCCAAACAGGTAAACTAGTCATAATCTTCATCCAAGGAATTCCAACTCCATTTTTCTTAACACCATTTCTCTCAACTCCAGTTTCCACATTTACATCCGAAACTTTCCTATCCATTTTCTTATTAACCGGAAAAAGCAATCCCCTGGCTCCCGAAGCCGAAGCTTTAGGATCAGAAGCATATCTAAACCAAAGCAAAGACCAAGCAAATCCTAAAAACGCTTCCGCGATAAAAACAGATTGAGGACCTTTAAACTTAACCAAACTCGGAAGAAACAACATTCCAACAGAAGCACCAAGATACATACCAGAAGCTTGAACGCCTGGTCTTTGTACTCTAATAGCAACAACCTGCCCTGTTCTGCGAAGCCTAGTTTGATAAACCTGCCCGAGTGAAGCAGCAACAACAGGCTTGGGTGAAATCCCTGAGAAAATATCAGCCAAGAATAAACCAAGTTCTTGTTCAATCATGTTGAAAGCAACTTCAGTGGAGAATGGACTTATTCGGTCTTGCAGTAGTGAGGGCTCATCTAGACATGATGGTGGTATCAAATCAGAACGAGATGATATAGCCTAAACAATTTTGGTATATGCCGGCCCAAGCTCCACCAATATCTTCCTTAGCCCTGCAGCTCTAACCTGAAACACATCATCAGAGAGATCAAATCGAGCGTCCAGATAGCGTAAACCAAACCACTTGCCAAAAGCAGTTCCAGTCTGAAACAATCTTCTAGCAACAACAAGCGGCTTCCTGAAGTAAATAGCCGCAATTTTCGATATACTATAATCGGCCAGAGAATCAACTTCCGTCGCGCTGAGTTCAAACAAGTAACCTGACTTCGCCGTAAAGGAGTCACCGCTATCGGAGCCAATCGAGACGGCGGTGCATGTGACGGAGCAACGACGACGACGAAGTTCCAATCGATAAACATAGTTCCGGCGGTGGTCGTGTTGGTACGACGAGGGTTTTGTTGTTGGTGGTTGCGGGAATAGATGGAAGTTATAGATCCGACGTCGTTCGTTGAGTAGATCGATGGTGGAAGGTTGTGTTTGGGGTGGTGAAGACGGATGGTTTTGACGGTAGATGGTGGGTTTGCGGCGGCTGAGGGTTTGTAAGGTTGTTGTTGTTTTATTTTTGTGTGTTTAGAAGACGAACGAAAAAAGGGAGGTCGCCAGCAGAGTGAGGTAGAGCGGAGACAGTTGAGGATGATGGTTTTGATGGAGGAAAGGGAGCGGTTGAGTGGGGGAAGAGGATGAAGGAGTCCCGTCTTGGGTGGCGGTCGGAGGAATACAACGGAGGGTATGATGAGTAGAGAGTGGATGTTGCGGAGATGAAGGTGAAGGGTTTGTGGAGGAGGGAGGCGGATATGCGGTGGTTTTGTGGAGTGAAGAAGAGGAAAGGTGGTTTTTTTGTTTTATCTTTTCAGAGGAGCGAGAGAGAAGAGAGAGGGTCAACAATTGAGAGTGGTGGGTGGGTAGGTATAGGGGGGACGCGTGTGAGGAGGCGCCGCTTAGGATTCACCATGTCTCYC
TARGET=1510,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03722 Ps_contig_mira-and-tgicl-ass_franssen_30845
SEQUENCE=CGGGGTCTACTAAAGTAAAANTAAACACTTGTTCTGGCCAATATTCAATTTTGAATATATAACACAGAAAATGAAATTCAAGTYGCGGTAACATAATTTATAGTCGTCGCTGAGTCGTAGTTTCTTTCTCTCTCTCACTCCCATCACACTCACAAACAAGTCAAGAAGAGTGAAGAAGAAGACGCAGATCGAAAATGGCGGAATCGAAGAAGAACGATATCGTTCATTCACCGCTCGTCACCTATGCTTCCATGATTTCTCTCCTCACGCTTTGTCCTCCTTTCGTAATTCTACTATGGTATACAATGACGGTTGCGGATGGATCAGTTTTGAACACTTTCGATTACTTTAGGAATAACGGTTTACAAGGTTTTGTTGATTTATGGCCTAAGCCTACTCTTCTCGCTTGCGAAATTATTGCTGTTTATGGTGTTTTTGAAGCTGCACTTCAGCTTCTTCTTCCTGGTAAAACTGTTTACGGCCCTATTTCTCCCACTGGCAATCGACCTGTTTATAAGGCAAATGGTGTTGCTGCGTATTTAGTCACTTTGGTTACTTATGTTGCTCTTTGGTGGTTTGGGATATTCAACCCAACAATCGTTTATGATCATTTGGGAGAGATATATTCAACACTCATCTTTGGGAGCTTAATCTTTTGTGTTTTCTTATACATAAAAGGTCATTTGGCACCATCTTCTACTGATTCTGGCTCATCTGGGAACATAATCATTGATTTTTACTGGGGGATGGAGCTATATCCACGCATTGGAAAATATTTTGACATAAAAGTTTTCACAAACTGCAGATTTGGAATGATGTCCTGGGCTGTTCTTGCACTTACCTATTGCATAAAGCAGTATGAAGAAAATGGGAAAGTAGCTGACTCAATGCTTGTAAATACTACACTGATGCTGGTGTATGTAACCAAGTTTTTCTGGTGGGAAGCTGGATACTGGAACACAATGGATATTGCACATGATCGAGCTGGATTTTATATTTGCTGGGGCTGTTTGGTTTGGGTTCCATCTGTGTATACATCTCCTGGAATGTACCTTGTCAACCATCCAGTAAATCTTGGCACCCAGCTAGCACTCTTTATTTTAGTGTCCGGCATTCTGTGCATATACATCAACTATGATTGTGACAGGCAAAGGCAAGAGTTTCGTAGAACAAACGGAAAAGGCTTGGTCTGGGGAAGAGCTCCATCAAAGATAGAGGCCTCATATACTACTACTTCCGGGGAAACAAAAAAAGCCTTCTTTTAACCTCTGGATGGTGGGGGTTAGCGCGTCATTTTCACTATGTCCCTGAAATACTAGCTGCATTCTTCTGGACAGTCCCAGCGCTTTTCAGTCATTTTTTGCCATACTTCTACGTGATATTTCTCACCATCCTTCTTTTTGATCGAGCGAAAAGGGATGATGATCGGTGCAGATCCAAGTATGGCAAGTACTGGAAACTCTATTGTGACAGGGTACCGTACAGAATCATTCCAGGAATATATTGAAGAATGATTATTGAAGATGACTTATCTGGAATCCTTCAATTGGTGCCATGTACCTTTGTTGTTTAATGCCTCTGATTGGTGAACTTTAGATGCAATGGTAGACTGTAGTTGTAAGAAATAATTCAGTAGTTACGGAACTCTCTTTTGTAAGACATAGAGACTTGGTTCTAGAGCTGTTAATCAATAATGTCAAACGACTCCTACAGGCTGCTTCTGATTTTTTACATTTTAAGAGAATTACGGAAATTGTTTTGACTTAAAAAAACT
TARGET=127,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03723 Ps_contig_mira-and-tgicl-ass_franssen_30850
SEQUENCE=GTCGGGGTATAGAAAGGTGTATGTTTAGACTAGAACATTCTGGTTTACAAAGAAAGCGTTAAGAAACCGACGTTACCGTGTTTGTTAATTGTTTTCTGTGGAGGTGCTTTTCTTTCCCTTTTTCTATTATTAACACATTCCAATCCCAATTTCTTTCCATTCCTTCTCTTTCTTTTCCTTTCCACTACGCATATTTTCTTCTCTCGATTTTCCTTTTTCTTCCTCAAAGGAAAGGGAAAATCTTCAAATCTTCAAATCTCTTCGATTCGAATCCTACAGATTTCTTTTCAATGGCGGAAGAACATAGATGCCAAGCTCCGCGTCTCTGCGCCAACAACTGCGGTTTCTTCGGGAGTCCAGCCATGCAAGATCTCTGTTCTAAATGTTACCGAGATTTGCAGCTCAAGGAACAGCGATCTTCGTCGGCGAAATTGGTTCTCAGTCAAACCCTAATTCCTCAGGCGGTGGTTTCACAAGAGGTTGTTGTTGTTCAGCCTTCTTCTTCGGAGGTGGTTTCGTCTTTGCCGGCGGTGGAAGCTGTTGCTGGAACATCGGAGCAGCCGAATCCGAATCGGTGCGGGAGTTGTAGGCGGCGCGTGGGGCTGACGGGGTTCAAGTGCAGATGCGGGTTGACGCTGTGTGGGTCCCACAGGTATCCGGAGCAACACGGGTGTGGGTTCGATTTTAAGGGAATGGGGAGAGAGCAGATCGCGAAAGCGAATCCGTTGGTGAAGGGAGAAAAGTTGAACAAGATCTGAACGGGGAAGGGTATTGTTGTAATTTCTGGTTTGTTACAGAAAAGGGAAAAGGGTTTGGATTTGGTTGTATGGGTCGGATTATCCGTTTCTCAGACGGATTTATTTGGGTGGGTGGGAGATTAGGGAATTGTCCTTTCATTTCGTTGTTATCATTTCATGTGTTAATAGTATTATGTGTTTAATAAAAACGTTTCATTTAGTTTTTCTATAANNNTCTATAATTGTTATTTTATTTTGTTTTTGTTT
TARGET=480,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7702
PRIMER_LEFT_PENALTY=0.629225
PRIMER_RIGHT_PENALTY=0.141011
PRIMER_LEFT_SEQUENCE=AAATTGGTTCTCAGTCAAACCC
PRIMER_RIGHT_SEQUENCE=CTCTCCCCATTCCCTTAAAATC
PRIMER_LEFT=428,22
PRIMER_RIGHT=701,22
PRIMER_LEFT_TM=59.371
PRIMER_RIGHT_TM=60.141
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.7702
PRIMER_LEFT_1_PENALTY=0.629225
PRIMER_RIGHT_1_PENALTY=0.141011
PRIMER_LEFT_1_SEQUENCE=AAATTGGTTCTCAGTCAAACCC
PRIMER_RIGHT_1_SEQUENCE=TCTCTCCCCATTCCCTTAAAAT
PRIMER_LEFT_1=428,22
PRIMER_RIGHT_1=702,22
PRIMER_LEFT_1_TM=59.371
PRIMER_RIGHT_1_TM=60.141
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=1.0350
PRIMER_LEFT_2_PENALTY=0.629225
PRIMER_RIGHT_2_PENALTY=0.405773
PRIMER_LEFT_2_SEQUENCE=AAATTGGTTCTCAGTCAAACCC
PRIMER_RIGHT_2_SEQUENCE=ATTCCCTTAAAATCGAACCCAC
PRIMER_LEFT_2=428,22
PRIMER_RIGHT_2=693,22
PRIMER_LEFT_2_TM=59.371
PRIMER_RIGHT_2_TM=60.406
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=1.1296
PRIMER_LEFT_3_PENALTY=0.988635
PRIMER_RIGHT_3_PENALTY=0.141011
PRIMER_LEFT_3_SEQUENCE=AATTGGTTCTCAGTCAAACCCT
PRIMER_RIGHT_3_SEQUENCE=CTCTCCCCATTCCCTTAAAATC
PRIMER_LEFT_3=429,22
PRIMER_RIGHT_3=701,22
PRIMER_LEFT_3_TM=59.011
PRIMER_RIGHT_3_TM=60.141
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=1.1296
PRIMER_LEFT_4_PENALTY=0.988635
PRIMER_RIGHT_4_PENALTY=0.141011
PRIMER_LEFT_4_SEQUENCE=AATTGGTTCTCAGTCAAACCCT
PRIMER_RIGHT_4_SEQUENCE=TCTCTCCCCATTCCCTTAAAAT
PRIMER_LEFT_4=429,22
PRIMER_RIGHT_4=702,22
PRIMER_LEFT_4_TM=59.011
PRIMER_RIGHT_4_TM=60.141
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_03724 Ps_contig_mira-and-tgicl-ass_franssen_30850
SEQUENCE=GTCGGGGTATAGAAAGGTGTATGTTTAGACTAGAACATTCTGGTTTACAAAGAAAGCGTTAAGAAACCGACGTTACCGTGTTTGTTAATTGTTTTCTGTGGAGGTGCTTTTCTTTCCCTTTTTCTATTATTAACACATTCCAATCCCAATTTCTTTCCATTCCTTCTCTTTCTTTTCCTTTCCACTACGCATATTTTCTTCTCTCGATTTTCCTTTTTCTTCCTCAAAGGAAAGGGAAAATCTTCAAATCTTCAAATCTCTTCGATTCGAATCCTACAGATTTCTTTTCAATGGCGGAAGAACATAGATGCCAAGCTCCGCGTCTCTGCGCCAACAACTGCGGTTTCTTCGGGAGTCCAGCCATGCAAGATCTCTGTTCTAAATGTTACCGAGATTTGCAGCTCAAGGAACAGCGATCTTCGTCGGCGAAATTGGTTCTCAGTCAAACCCTAATTCCTCAGGCGGTGGTTTCACAAGAGGTTGTTGTTGTTCAGCCTTCTTCTTCGGAGGTGGTTTCGTCTTTGCCGGCGGTGGAAGCTGTTGCTGGAACATCGGAGCAGCCGAATCCGAATCGGTGCGGGAGTTGTAGGCGGCGCGTGGGGCTGACGGGGTTCAAGTGCAGATGCGGGTTGACGCTGTGTGGGTCCCACAGGTATCCGGAGCAACACGGGTGTGGGTTCGATTTTAAGGGAATGGGGAGAGAGCAGATCGCGAAAGCGAATCCGTTGGTGAAGGGAGAAAAGTTGAACAAGATCTGAACGGGGAAGGGTATTGTTGTAATTTCTGGTTTGTTACAGAAAAGGGAAAAGGGTTTGGATTTGGTTGTATGGGTCGGATTATCCGTTTCTCAGACGGATTTATTTGGGTGGGTGGGAGATTAGGGAATTGTCCTTTCATTTCGTTGTTATCATTTCATGTGTTAATAGTATTATGTGTTTAATAAAAACGTTTCATTTAGTTTTTCTATAANNNTCTATAATTGTTATTTTATTTTGTTTTTGTTT
TARGET=863,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4004
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=1.388237
PRIMER_LEFT_SEQUENCE=GTTGGTGAAGGGAGAAAAGTTG
PRIMER_RIGHT_SEQUENCE=ACGAAATGAAAGGACAATTCCC
PRIMER_LEFT=724,22
PRIMER_RIGHT=901,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=61.388
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=1.5292
PRIMER_LEFT_1_PENALTY=0.141011
PRIMER_RIGHT_1_PENALTY=1.388237
PRIMER_LEFT_1_SEQUENCE=ATTTTAAGGGAATGGGGAGAGA
PRIMER_RIGHT_1_SEQUENCE=ACGAAATGAAAGGACAATTCCC
PRIMER_LEFT_1=681,22
PRIMER_RIGHT_1=901,22
PRIMER_LEFT_1_TM=60.141
PRIMER_RIGHT_1_TM=61.388
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=1.5292
PRIMER_LEFT_2_PENALTY=0.141011
PRIMER_RIGHT_2_PENALTY=1.388237
PRIMER_LEFT_2_SEQUENCE=GATTTTAAGGGAATGGGGAGAG
PRIMER_RIGHT_2_SEQUENCE=ACGAAATGAAAGGACAATTCCC
PRIMER_LEFT_2=680,22
PRIMER_RIGHT_2=901,22
PRIMER_LEFT_2_TM=60.141
PRIMER_RIGHT_2_TM=61.388
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=1.6705
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=1.658345
PRIMER_LEFT_3_SEQUENCE=GTTGGTGAAGGGAGAAAAGTTG
PRIMER_RIGHT_3_SEQUENCE=CGAAATGAAAGGACAATTCCC
PRIMER_LEFT_3=724,22
PRIMER_RIGHT_3=900,21
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=60.658
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=1.7994
PRIMER_LEFT_4_PENALTY=0.141011
PRIMER_RIGHT_4_PENALTY=1.658345
PRIMER_LEFT_4_SEQUENCE=ATTTTAAGGGAATGGGGAGAGA
PRIMER_RIGHT_4_SEQUENCE=CGAAATGAAAGGACAATTCCC
PRIMER_LEFT_4=681,22
PRIMER_RIGHT_4=900,21
PRIMER_LEFT_4_TM=60.141
PRIMER_RIGHT_4_TM=60.658
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_03725 Ps_contig_mira-and-tgicl-ass_franssen_30862
SEQUENCE=TCGGGGAACCTTCTYCCCTTTTGATATATATCTTTTTCTAACTCTCTCTTCGACCTCTCTTTCTTTCTTCTTTCTTCTTTCCCAATTCCCAATCACTCTCTTATCCCAAACGCCATCGCTAAACGCCGTTTAGGGTTTCAGGTTGAAATAAGTGGTTTGTTATCGTCAAAATGTCATCTCCAAGCAAGAGACGGGAAATGGACGTAATGAAATTGATGATGAGTGATTATACAGTGGAGACGATAAATGATGGACTCACAGAATTCAATGTGGAGTTTAATGGTCCAAAAGAAAGCCTTTATGAAGGCGGAGTTTGGAAGATTCGTGTTGAACTTCCTGATGCATATCCGTACAAATCCCCTTCTATCGGCTTCGTGAACAAAATATACCACCCGAATGTTGATGAGTTATCTGGCTCTGTATGCTTGGACGTAATTAACCAATCATGGAGTCCAATGTTTGATCTTCTAAATGTCTTTGAAGTTTTTCTTCCACAACTCTTGCTTTATCCAAACGCCTCAGATCCTTTGAATGGCGACGCCGCATCCTTAATGATGAAGGATAGAAAGTTGTATGACCAAAAAGTTAAAGAGTATTGTGAGCGGTATGCTAAGAAGGAAAACATTTCCAACAATGGTACAGCTGATGATGAGGACAGTGATGAGGAAGACATCAGTGAAGCAAATACCCAATCAAGTGATGATGAAATTCCTGGTCATGCTGACCTTTAAATAGTGCTCTTTAAGTTTAAGGGTCCATGTATTATTACACATATTTACTTGTTTAGTTATAATTATAAGATGTAGGGTTATTTCTCTGTACTACTTTTCTGCCCTTATAATAACAGTTATTATTCCTGTATAATAATTTCTTATAAAGCTATACTATAATATGTTATTAGGGCTTTAAACTTTAAATTGTAATTTGTATATTAATATCTGACATTTTTCTTTCCAAATTTAAGGAAATCAGAAATGTAACAGTTAAAGAYRCATGCTGAAGTAA
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03726 Ps_contig_mira-and-tgicl-ass_franssen_30873
SEQUENCE=AAGCAGGTGGNTATTCAACGCAGAGTGGGGGAAAGTTTAAAAATCGCATCCAGAAGAAAGTTGATGCAAAGTATGCAGCTGGCCTTTTAGCATCAAAGGAAGCACAAGAAGAGATCTTAGATTATGAGAAAAGAAAAGACAACGCCACAGACACTCTTGATTCTTTATACAACGAAATACGGCCTGCCATTGAAGAACACGAGAGGGATACCCAAGACAGTGTATCTACAACCGTAGCCGAGAAGTGGATTCAGGCATGCTGTCTAAAACTGAAAGCGGAGTTTGATCTTTACTCATCAATTGTTAAAAATGTTGCTTGTACTGCTCAAAAGGCACCTGGTCCAGCAAAAGCAACCGAAGTAGATAACGAAAACAAAAATAAGCTTTTGAGTGATTGAAATTCCTATCACATTTCACCCTAATCTAATCTAATCTAATCTGTAATCGACGATTTACATAACATATGGACAACTTTGAGACAACAAATCATAATCTTGTTCATTACAGTATAATTTTGACATGTTATTGCTAGTTACAAATTCCTTTTTTGTTTTATGNAACCTGTTAATTTTGTCACTTTCATGAAATTCTAT
TARGET=419,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03727 Ps_contig_mira-and-tgicl-ass_franssen_30882
SEQUENCE=TTAAGAATTGTGGGGGCGTTCTTGGTGATGGCTTTGGTTCTACGCCTGAAATGTGCAAAAAGAACTTGATATGGGTAGTTGCGCGGATGCAAGTTGTTGTTGATCGTTATCCTACGTGGGGAGATGTTGTTCATGTAGAAACTTGGGTGTCTGCATCGGGCAAGAATGGTATGCGTCGTGATTGGCTTTTACGCGACTATAATACTGGTGAAGTCTTGACGAGAGCCTCTAGTGTTTGGGTCATGATGAATAAGCATACCAGAAAGCTGTCCAAAATTCCAGAAGAAGTGAGAGAGGAGATAGGATCTTTTTTTGTGGAATCTGCTCCAATTCTGGAAGAGGATGACAAAAAACTCACGAAACTCGATGACAGTACAGCGGATTATATCCGTTCGGGTTTAAGTCCTAGATGGAGTGACCTAGATGTCAATCAGCATGTTAACAATGTGAAGTACATTGGCTGGATTCTGGAGAGTGCTCCACAAGCAATCTTGGAGAGTCATGAGCTTTGTGGCATGACTATGGACTACAGGAGGGAGTGTGGCAAGGACAGTGTTCTACAGTCCCTGACCGCTGTATCTGATGCTAATGTTGGCAATCTAGCTCAAAGCGGCCACATTGAGTGCAAGCATTTGCTTCGACTTGAAGATGGTGCTGAAATTGTGAGGGCTAGGACTAAGTGGAGGCCAAAACCTGTGAACAAGTTTGATTTTGCGAATCAGGTGCCAGCAGGTAGCACCTAAGGTTTTCGAATGGTTGATGGTTGGAGTGGCATCACATGAGTTTCCATTCTATTATTAAACTGGTTCTGACCTCAGAAGAGTTTTGCTTGTGTTTGTCCAGTCTACATGTCTTATATACATGTCTTATACCTTCTAATTTGTGTTGCTATGGTGGGTAAAAAGGGGTGAAAGTACCATAAATCTCATTGTAATTAGCTCCTGCTCTGCTCTGCTCTTATCTCTCTCTGCACCTCCAAATTTCATTTATTTTCTGGTTGCTCTAGTGCTAGGCTGGCTTTCTTCAATATTTAATTGTTTGATCAAAATGGCTAGGCATGTATATTATTATTCTTTTCTCTTGGCCCATCTAAGATGCAAATTTCCTTGTGAACATAATAGCACAATAATTATTAGCCAGTAGGCAGAAATGAATTTTCCGTCTAATACAACTGTGAATTGTATGCTCCAGCTTGTATGTATGCCAACTTCCCACATGRGATGGAATTATTGTTTAGCAAT
TARGET=942,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03728 Ps_contig_mira-and-tgicl-ass_franssen_30885
SEQUENCE=AATTTTAAAATTTAATTACAATAACGTACCCAAAATAATACCAAACACACTATTTTGTAACTAAAAAAAAACTAAACTAAGTAATTACAGTAAAAAAAAATTGCTACATATGGAAATAGATTCCTAATGACCAAAACCAAGCAATTAGGATGAAATCACTTTGGACTTAGTCACGAAAATAGGTTGTCTTGAAGCTTGGAGCAGCCACCTTCCATTCCAGCAGGTCATCTCAGAGTTGTAATCTGCAGAGAACAAAAATAGCTTATTTTAAAGCACTTTTCAAAAGCACTTATAGAGCTGAAAAACAACTAAATGAATCATTCAGTTTGAAGCTTTATCCAGCAGGTTAACATGAATACTCAAAAAAGCACTTTTGACATTAGCTTAAAACAGAACAAATCAATAGAAATTAAATCCCACATAATCTGGGATGGGACAAATGGAAAGAGGAGTTTTTTTAGACTTAACAAATCCTTCATAAACGGATCCAAAAGGAGGTGAGCAAATAGAGACAGTAAGATATTTTTCTCAGGATACCAAAAAAAACAAGAACCACACAACAAGATACACAACACGAATTTTTCTAAAACCCGAAACAAGAACGCACAACAATATTTCAAAGAGAACCTTAAAGAACCAACAAACAGCAAAAACATCCAGAATAGTTCGTTTTTCTTNGAACCCAAACACACAGCAARATACTTTCAAAAAATCYTTAAAAAAAGTTAAATACAGCAAGAACAACGGAGCGATTTTTTGCAAACCCAGAACACAACAGCCATAACATTTCTCGAAAACCCTAAGAATGAAAACAGAAGGAGAAAGCTTATCTGGAGACGGGAGGGAGAAGGCATCATCGGCGGCGTGCTTCCGAACAAGATCAACACAAACCACGCTGAAGGACCCTCCGTCGAAGCTTCGAGTACGATTGATTTCGGTTCCTTTCTCAAGCTTCCTATTTATCCATCTTGATCTCATTTCGTTAGCCTAAAAGCTTTGYTTGTTCTGCCTAACTTTTGTTTGTTTGGTGTGGGTGAGTTTAGATATGTTGGTTTAAGGTTTTTTTGGGGTCAAAATAATGGTCGGATCCGTAACCGCGAGTGTANAGAGATATGTTGGTTTGGTTTTGGGTTAGGTAG
TARGET=1016,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03729 Ps_contig_mira-and-tgicl-ass_franssen_30886
SEQUENCE=AATATAAATACCCAAACCTTTCTCTTCTRTCGGGAGGCTCCATCATCATCATCATCAACCTAACCAACCAAATCCTCTCTCCCTCTCTCTCTCACCAAAAAATGGCTCTCAAAGCCGTCCACGTCTCCGACGTTCCCTCCCTCGACCTCGTGCCAGAAAATCCCTCCCTCTCCCTCCTCAACCGTTTCCCCAACGGTTTGGAGATGAGCAATGGCGGATTCAAATTGCCCAAGTTCGTTGTGATCGGACACAGAGGAAACGGAATGAATGTTATTCAATCTTCTGATCGGAGAATGAGAGCCATCAAAGAAAACTCCATCATGTCTTTCAACGCCGCTTCTTCTTTTCCTCTTGATTTCATTGAATTCGACGTTCAGGTCACCAGAGATGACTGCCCAGTCATTTTCCATGATGATTACATCTACTCTCAGGAAAATGGTAATGTGTTTGGGAAGAGAATACCTGAGTTGTGTCTATCTGAGTTTCTTTCCTATGGGCTTCAAAGAGAGGCTGGAATGGAAGGAAAAGTTTTGGTAAGAAAAACAAAAGATGGAAGGATATGTAATTGGGAAGTTGAACAAGATGACACACTTTGTACCCTTCAAGAAGCGTTTCTCAAAGTTGAACCTTCTTTGGGTTTTAACATTGAGTTGAAATTTGATGACCATATTGTTTATGAGCAAGCTTATCTTATTCATGTCCTTCAAACCATCTTAAAGGTTGTTATGGATTATGCCAAGGATAGGCCTGTTATATTCTCTACTTTTCAACCTGACGCTGCTATACTTGTTAGGAAATTGCAAAGAACATACCCTGTTTTCTTTTTGACTAATGGAGGGAGTGAATTTTATGAAGATTTGAGGAGAAACTCCTTGGAAGAAGCCTTGAAGCTTTGTTTGGAAAATGGATTGGAAGGAATTGTCTCTGAGGTTAAAGGGATATTTAGAAATCCAGGAGCAGTAAGCAAGATCAAGGAGTCCAATCTTTCTCTTTTAACATATGGAAAATTGAATAATGTTCCAGAAGCGGTTTACATGCAGCACCTAATGGGAATTGATGGGGTGATAGTAGATTTTGTGCAAGAGATAACAGAAGCAGTTGCAGATATGATGAACATACAAAATGGYGAGGAAGAGGAGTGAGTGAAGGAATTGGGAAGGTGGAA
TARGET=41,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03730 Ps_contig_mira-and-tgicl-ass_franssen_30886
SEQUENCE=AATATAAATACCCAAACCTTTCTCTTCTRTCGGGAGGCTCCATCATCATCATCATCAACCTAACCAACCAAATCCTCTCTCCCTCTCTCTCTCACCAAAAAATGGCTCTCAAAGCCGTCCACGTCTCCGACGTTCCCTCCCTCGACCTCGTGCCAGAAAATCCCTCCCTCTCCCTCCTCAACCGTTTCCCCAACGGTTTGGAGATGAGCAATGGCGGATTCAAATTGCCCAAGTTCGTTGTGATCGGACACAGAGGAAACGGAATGAATGTTATTCAATCTTCTGATCGGAGAATGAGAGCCATCAAAGAAAACTCCATCATGTCTTTCAACGCCGCTTCTTCTTTTCCTCTTGATTTCATTGAATTCGACGTTCAGGTCACCAGAGATGACTGCCCAGTCATTTTCCATGATGATTACATCTACTCTCAGGAAAATGGTAATGTGTTTGGGAAGAGAATACCTGAGTTGTGTCTATCTGAGTTTCTTTCCTATGGGCTTCAAAGAGAGGCTGGAATGGAAGGAAAAGTTTTGGTAAGAAAAACAAAAGATGGAAGGATATGTAATTGGGAAGTTGAACAAGATGACACACTTTGTACCCTTCAAGAAGCGTTTCTCAAAGTTGAACCTTCTTTGGGTTTTAACATTGAGTTGAAATTTGATGACCATATTGTTTATGAGCAAGCTTATCTTATTCATGTCCTTCAAACCATCTTAAAGGTTGTTATGGATTATGCCAAGGATAGGCCTGTTATATTCTCTACTTTTCAACCTGACGCTGCTATACTTGTTAGGAAATTGCAAAGAACATACCCTGTTTTCTTTTTGACTAATGGAGGGAGTGAATTTTATGAAGATTTGAGGAGAAACTCCTTGGAAGAAGCCTTGAAGCTTTGTTTGGAAAATGGATTGGAAGGAATTGTCTCTGAGGTTAAAGGGATATTTAGAAATCCAGGAGCAGTAAGCAAGATCAAGGAGTCCAATCTTTCTCTTTTAACATATGGAAAATTGAATAATGTTCCAGAAGCGGTTTACATGCAGCACCTAATGGGAATTGATGGGGTGATAGTAGATTTTGTGCAAGAGATAACAGAAGCAGTTGCAGATATGATGAACATACAAAATGGYGAGGAAGAGGAGTGAGTGAAGGAATTGGGAAGGTGGAA
TARGET=83,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03731 Ps_contig_mira-and-tgicl-ass_franssen_30890
SEQUENCE=TCCTTNGNNGACTCTCTAATCTCATCCCAATTCTCACTCTCACTCACTCACCACCACCATGGACCCAACTTCCTTCGATCTCCCTCCACCACCACCCACCACCACCACCACCGTCCCCTTCTCCCTGGAACCCAACTTCACCTCCTCCGTCACTCACCCTCCTTACGCCGAGATGATATACAAAGCAATAGAGTCACTAAAAGAGAAAGACGGTTCAAGCAAAAGAGCAATAGGAAAATTCATAGAACAAAACTACGGTAACGTTCTTCCACAACAACACACCACTCTCTTGACTCAACATCTCAACCACTTGAAATCTGCTGGTCTTCTCATCACCTTCAAAAAATCTTACAAACTACCTTCCTCACTCCCCCCACCTCCAACCGTCACTAGATCTGACTCCGAATCTCATCTCCCTTTCACCTCCACTTAAACCNAAAAACGACCCGTCGGCCGTCCCCCGAACGCCNANGCCCGAACTTAATTCCGAACCCGAACTAACTCGCCCACGTCCGGTTTTCGTTTCTCTACGACCCTCCTACTCACGACGACGCCTCCA
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.8805
PRIMER_LEFT_PENALTY=2.807998
PRIMER_RIGHT_PENALTY=0.072552
PRIMER_LEFT_SEQUENCE=AATCTCATCCCAATTCTCACTC
PRIMER_RIGHT_SEQUENCE=CTTTTGCTTGAACCGTCTTTCT
PRIMER_LEFT=17,22
PRIMER_RIGHT=224,22
PRIMER_LEFT_TM=57.192
PRIMER_RIGHT_TM=59.927
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=2.8805
PRIMER_LEFT_1_PENALTY=2.807998
PRIMER_RIGHT_1_PENALTY=0.072552
PRIMER_LEFT_1_SEQUENCE=AATCTCATCCCAATTCTCACTC
PRIMER_RIGHT_1_SEQUENCE=CTCTTTTGCTTGAACCGTCTTT
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=226,22
PRIMER_LEFT_1_TM=57.192
PRIMER_RIGHT_1_TM=59.927
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=2.9194
PRIMER_LEFT_2_PENALTY=2.807998
PRIMER_RIGHT_2_PENALTY=0.111365
PRIMER_LEFT_2_SEQUENCE=AATCTCATCCCAATTCTCACTC
PRIMER_RIGHT_2_SEQUENCE=AGAGAGTGGTGTGTTGTTGTGG
PRIMER_LEFT_2=17,22
PRIMER_RIGHT_2=289,22
PRIMER_LEFT_2_TM=57.192
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=3.0882
PRIMER_LEFT_3_PENALTY=3.015613
PRIMER_RIGHT_3_PENALTY=0.072552
PRIMER_LEFT_3_SEQUENCE=TCTAATCTCATCCCAATTCTCACTC
PRIMER_RIGHT_3_SEQUENCE=CTTTTGCTTGAACCGTCTTTCT
PRIMER_LEFT_3=14,25
PRIMER_RIGHT_3=224,22
PRIMER_LEFT_3_TM=59.984
PRIMER_RIGHT_3_TM=59.927
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=3.0882
PRIMER_LEFT_4_PENALTY=3.015613
PRIMER_RIGHT_4_PENALTY=0.072552
PRIMER_LEFT_4_SEQUENCE=TCTAATCTCATCCCAATTCTCACTC
PRIMER_RIGHT_4_SEQUENCE=CTCTTTTGCTTGAACCGTCTTT
PRIMER_LEFT_4=14,25
PRIMER_RIGHT_4=226,22
PRIMER_LEFT_4_TM=59.984
PRIMER_RIGHT_4_TM=59.927
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_03732 Ps_contig_mira-and-tgicl-ass_franssen_30890
SEQUENCE=TCCTTNGNNGACTCTCTAATCTCATCCCAATTCTCACTCTCACTCACTCACCACCACCATGGACCCAACTTCCTTCGATCTCCCTCCACCACCACCCACCACCACCACCACCGTCCCCTTCTCCCTGGAACCCAACTTCACCTCCTCCGTCACTCACCCTCCTTACGCCGAGATGATATACAAAGCAATAGAGTCACTAAAAGAGAAAGACGGTTCAAGCAAAAGAGCAATAGGAAAATTCATAGAACAAAACTACGGTAACGTTCTTCCACAACAACACACCACTCTCTTGACTCAACATCTCAACCACTTGAAATCTGCTGGTCTTCTCATCACCTTCAAAAAATCTTACAAACTACCTTCCTCACTCCCCCCACCTCCAACCGTCACTAGATCTGACTCCGAATCTCATCTCCCTTTCACCTCCACTTAAACCNAAAAACGACCCGTCGGCCGTCCCCCGAACGCCNANGCCCGAACTTAATTCCGAACCCGAACTAACTCGCCCACGTCCGGTTTTCGTTTCTCTACGACCCTCCTACTCACGACGACGCCTCCA
TARGET=96,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2286
PRIMER_LEFT_PENALTY=0.156061
PRIMER_RIGHT_PENALTY=0.072552
PRIMER_LEFT_SEQUENCE=TCCCAATTCTCACTCTCACTCA
PRIMER_RIGHT_SEQUENCE=CTTTTGCTTGAACCGTCTTTCT
PRIMER_LEFT=24,22
PRIMER_RIGHT=224,22
PRIMER_LEFT_TM=59.844
PRIMER_RIGHT_TM=59.927
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=201
PRIMER_PAIR_PENALTY_1=0.2286
PRIMER_LEFT_1_PENALTY=0.156061
PRIMER_RIGHT_1_PENALTY=0.072552
PRIMER_LEFT_1_SEQUENCE=TCCCAATTCTCACTCTCACTCA
PRIMER_RIGHT_1_SEQUENCE=CTCTTTTGCTTGAACCGTCTTT
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=226,22
PRIMER_LEFT_1_TM=59.844
PRIMER_RIGHT_1_TM=59.927
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.2674
PRIMER_LEFT_2_PENALTY=0.156061
PRIMER_RIGHT_2_PENALTY=0.111365
PRIMER_LEFT_2_SEQUENCE=TCCCAATTCTCACTCTCACTCA
PRIMER_RIGHT_2_SEQUENCE=AGAGAGTGGTGTGTTGTTGTGG
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=289,22
PRIMER_LEFT_2_TM=59.844
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.3600
PRIMER_LEFT_3_PENALTY=0.353013
PRIMER_RIGHT_3_PENALTY=0.006945
PRIMER_LEFT_3_SEQUENCE=TCACTCTCACTCACTCACCACC
PRIMER_RIGHT_3_SEQUENCE=TGAGAAGACCAGCAGATTTCAA
PRIMER_LEFT_3=33,22
PRIMER_RIGHT_3=331,22
PRIMER_LEFT_3_TM=60.353
PRIMER_RIGHT_3_TM=59.993
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.4256
PRIMER_LEFT_4_PENALTY=0.353013
PRIMER_RIGHT_4_PENALTY=0.072552
PRIMER_LEFT_4_SEQUENCE=TCACTCTCACTCACTCACCACC
PRIMER_RIGHT_4_SEQUENCE=CTTTTGCTTGAACCGTCTTTCT
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=224,22
PRIMER_LEFT_4_TM=60.353
PRIMER_RIGHT_4_TM=59.927
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_03733 Ps_contig_mira-and-tgicl-ass_franssen_30891
SEQUENCE=GGTTTTTTACTNTNNNTTTTCTTCTAATAACATCAAAGTCTTAATATTATTTTACAGAAGATTATCCTGTTATTCAATCCTGGTGATAAGACTTCATATCATTTATGCATGTATGAAGCGTTAATTTAGAATAATGGCAGCAAAATTAGCAGCTAGAAGCCACTGAACCGGTATACATACAAACCTAACTGGTACATAATATAAGCCATTTTGAACTCTACTCTAGACCCCCCAAAAAAACTCATTATGTATTCTAGTCCGTATCACTATCCGCAAATATCTCAGGAGTAGGCTCAGCTTCATCGGTTGTTGATTTCTCTTCTTCCTCTCTTCTTTTCTCCTCCTCCTCTCTCTCCCTCTCTGCAATTATGGCCTTAGCTTTCTCAAGTCTAGAGCTGACTGCTTCCCAAGACACAAGCTTATCCATGAACACTGATATATAATCAGGGCGCCGGTTCTGATAGTCAAGATAGTAAGCATGCTCCCATACATCAATGGTAAGGAGTGGATGATGATGGTTCCAAACAAGGGGGTTCACAGCATTAGGACTCTTCAGCACAACGAGTTTTTTATCCTCCTCAGTTGCAAGAGGATTTACTGCATTTCCAACATCAAGTCTACTTTCTTTATATGCAAGCCATGCCCAGCCTGAACCAAATTGTGTTGCAGCAGCAAGCTTGAACTGCTCAACAAATTTTTCAAATGAACCAAAGTCTCTTTCAATCAGTTTTAGAAGTTCCCCCGATGGCTTTCCGCCACCACCAGGTTTCATGGACTCCCAAAAGAAATCATGGTTCCATACCTGTGCTGCATTGTTGAAAGCCGGAAGAATGTCGCCATTATTGTACGCTATAACTATAGTTTCCTCTAGTGATTTCCCATCTAAATCTGTTTCATCAATTTGCTTGTTCAAGTTATCTACATAAGCTCTGTGGTGCTTTCCCCAATGGTACTCAAATGTATCCCGGCTCATAATTGGCTCCAAAGCATCCAGCGGATAAGGCGGTGGCTTCAGCTCAAACTTTGCAGTGATATTAGTGCTCCCGGCTTTCCTAATGCATCTTCCCTTATTCTTAGAGAATTTGAAGGTAGCTGAAGAAAAGCCCAGATTTTGGAACCCATTGGAGTGAGAAGGGAGAAAAGCGGAAGAAGGGAGGGAAGAAGTAGCGGTTGCGGAAGGCGATAGCAATTTCATCTCTTTGACACTCTCTCTGCTTATGCTTGTACTAAGGAGTTAGGAGATTCAAAGTATTTGGGCTCTTNGCATATTTGACCT
TARGET=338,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1611
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.052187
PRIMER_LEFT_SEQUENCE=CCGCAAATATCTCAGGAGTAGG
PRIMER_RIGHT_SEQUENCE=TCGTTGTGCTGAAGAGTCCTAA
PRIMER_LEFT=270,22
PRIMER_RIGHT=563,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=0.2045
PRIMER_LEFT_1_PENALTY=0.108929
PRIMER_RIGHT_1_PENALTY=0.095566
PRIMER_LEFT_1_SEQUENCE=CCGCAAATATCTCAGGAGTAGG
PRIMER_RIGHT_1_SEQUENCE=AGCTAAGGCCATAATTGCAGAG
PRIMER_LEFT_1=270,22
PRIMER_RIGHT_1=379,22
PRIMER_LEFT_1_TM=60.109
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=110
PRIMER_PAIR_PENALTY_2=0.2962
PRIMER_LEFT_2_PENALTY=0.243965
PRIMER_RIGHT_2_PENALTY=0.052187
PRIMER_LEFT_2_SEQUENCE=CGCAAATATCTCAGGAGTAGGC
PRIMER_RIGHT_2_SEQUENCE=TCGTTGTGCTGAAGAGTCCTAA
PRIMER_LEFT_2=271,22
PRIMER_RIGHT_2=563,22
PRIMER_LEFT_2_TM=60.244
PRIMER_RIGHT_2_TM=60.052
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.3065
PRIMER_LEFT_3_PENALTY=0.210886
PRIMER_RIGHT_3_PENALTY=0.095566
PRIMER_LEFT_3_SEQUENCE=AAGCCACTGAACCGGTATACAT
PRIMER_RIGHT_3_SEQUENCE=AGCTAAGGCCATAATTGCAGAG
PRIMER_LEFT_3=156,22
PRIMER_RIGHT_3=379,22
PRIMER_LEFT_3_TM=59.789
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.3183
PRIMER_LEFT_4_PENALTY=0.108929
PRIMER_RIGHT_4_PENALTY=0.209343
PRIMER_LEFT_4_SEQUENCE=CCGCAAATATCTCAGGAGTAGG
PRIMER_RIGHT_4_SEQUENCE=TTACCATTGATGTATGGGAGCA
PRIMER_LEFT_4=270,22
PRIMER_RIGHT_4=500,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=60.209
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_03734 Ps_contig_mira-and-tgicl-ass_franssen_30894
SEQUENCE=CTTCTATTAACCTTGCAACAGACTAGACAAAGCCAAAACTAAACGTACAAAACTTAAGTCAAGCTAAGTCTTCAAATTGATCTCAACATCAAGCCTCAAATGAATAATCACGTAATACAAACACCATAGAAAATGGTCATATTCTTGAAAAATTACAAAGCTTCACCTTGCATATTTGACGAAACAGGTAAGTGGTTGTGCAAAAAAATTATAACTCGAGCATCATGACTTTATGTGTTTCAATTGCGTAACCTTCTCAAAATCCAGAGGATGGAAGTTTCTTTCTACCTCCCAAGCCAATTGTGACATACAATTAAATGTTGACTTGGCCCCTTTTTCATCAGTGACTTCTCTAGGGAAAGGGATGCGGACCTCAAATAAACCGTTGTTAGGAGAAAACAAACGCATATCCAATCCCAAGCGATCTACCCATATCATCTTTGCTTCTGAGACCTGGAAATCCAAGTCAACATAGACATTACAAAAGCGAGTAATGTCTTCCATATTATTGGTGTTAATTTCAGCTACTAAATTCTCAGCAAATTCTCGAAGGGGGTCGGGTTGAGCATTTTTATAGTCTAAAGAGGTGAACCACACACCATCCTCCTGGAAGTCATCCAAATGCAGCACCCTATCTACATCAATAATATACATAAAATCTTGATCAACTTCTTCTCCGAACCTCTTCTTCCACAAAGAAACAAGCCGTTTGATCAAAACTGGATCCTGCGGTTTGGAAAGTGTTCCTTGAAGTGTACACTGAGGTGTACGCAATCCTGACTGTAAAAACTTAACATGAAGTGAAGAAGGGGCGTGAATGTTGTGATTGGCAGTGGGAACAGAATTATGATTGAAATAGAAAAAAGGGTTTCCGTTTTCAGAATCAACAGCGAATCGAACCCCAATACCAATTGGCCAACCCTGTTGAGTCAAAGTAGAAAGCGTGCCAGCCCTAGCTAAATCCATAATGGTTCTGGATACTTCAGCTGGAAATGGTTTGGTAATGTTGTTATCGGTTTTGAGTTCCAATTGGGTTGAAACTGAACACTTTGTTGAGCGAAATGAGATTGAATTTGAAGGGTGAGTAGAGATTTTAGTAGTAGGAGTCGTTATTTTGGGTGTGAAACATGGTGCAGTGAAGTTTCTAGTTTGAAGAAGCATTTTTTGTTTGTTTTGGGATTTTAGGGTTTCGTTGATTGATTATAGAAGATGATGAAATTGAATTGAATTGAAAGAGAAGTTGAAGATGGATTTGAGTGTGGAAGCAATAGGGAAAATGGATAAGAATTTGATACACGCGACAACAAAGTGTCGTTTCTACGTAAGTAAAATGTAAACGGCACTTCTGTTTTCTGCAGTGTTGTTAAGGATGCGTTTTAAGTCAAGAAAGT
TARGET=1217,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0427
PRIMER_LEFT_PENALTY=0.041981
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=AACTGAACACTTTGTTGAGCGA
PRIMER_RIGHT_SEQUENCE=TTTTCCCTATTGCTTCCACACT
PRIMER_LEFT=1038,22
PRIMER_RIGHT=1276,22
PRIMER_LEFT_TM=59.958
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.0427
PRIMER_LEFT_1_PENALTY=0.041981
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=ACTGAACACTTTGTTGAGCGAA
PRIMER_RIGHT_1_SEQUENCE=TTTTCCCTATTGCTTCCACACT
PRIMER_LEFT_1=1039,22
PRIMER_RIGHT_1=1276,22
PRIMER_LEFT_1_TM=59.958
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.0712
PRIMER_LEFT_2_PENALTY=0.041981
PRIMER_RIGHT_2_PENALTY=0.029253
PRIMER_LEFT_2_SEQUENCE=AACTGAACACTTTGTTGAGCGA
PRIMER_RIGHT_2_SEQUENCE=CCACACTCAAATCCATCTTCAA
PRIMER_LEFT_2=1038,22
PRIMER_RIGHT_2=1261,22
PRIMER_LEFT_2_TM=59.958
PRIMER_RIGHT_2_TM=59.971
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.0712
PRIMER_LEFT_3_PENALTY=0.041981
PRIMER_RIGHT_3_PENALTY=0.029253
PRIMER_LEFT_3_SEQUENCE=ACTGAACACTTTGTTGAGCGAA
PRIMER_RIGHT_3_SEQUENCE=CCACACTCAAATCCATCTTCAA
PRIMER_LEFT_3=1039,22
PRIMER_RIGHT_3=1261,22
PRIMER_LEFT_3_TM=59.958
PRIMER_RIGHT_3_TM=59.971
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.0815
PRIMER_LEFT_4_PENALTY=0.080808
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=GTGAAACATGGTGCAGTGAAGT
PRIMER_RIGHT_4_SEQUENCE=TTTTCCCTATTGCTTCCACACT
PRIMER_LEFT_4=1120,22
PRIMER_RIGHT_4=1276,22
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_03735 Ps_contig_mira-and-tgicl-ass_franssen_30904
SEQUENCE=ATGGNGAAAGGTAGGATGATTCTCAGGAGAGGGCGGAAAGTACTCCTTTGATTCGAAGAATGAACAAACGAAGGGTTTGGTAAGAAAGGAAGGTGACGATTGGTTGTCATTGGAACACAGAGACTTCGTACCCGACCCGAATATGGTTCCTAAACAGATCCGGATCGTTGATTTCAAGACAGGTTGCGAAGTTTACGCCGGATCCGCGTTCGCGATGTCTCCGTCACCGAACGCACTTCCGATTCCGTCTTTTCAGAGGAAGTTTGCGCCGGTGGCCTTCGACGACTCCGCAACACGGGATCTGAGACGTCTTCTTCGTATTGCATGAGTCTGAAACGAGTCATTTCCGGGTTACAGGTCATATCCATATCCACGTCAACTTTTCATCTTCTCTTCCTTTTTGTCTCTCTCTCTCTCCCTCTCTCTCTCTTTCTCTCCTTAATCTAGAATCTTCTTATTACCCGTTACCGTCCCTACTCCCTATTTTTCTAATTCTACTCACCGTGTATTTTTTAAACGGTAAGGGTGAATTAAAAATAGGGTAAACGTCGGGGCGGAAATGTTAAATTAAAAAAGTCTGGAGTAGTGTGGTTGAGTAGGTTTTATGAAGATCTTTGTTTAAATTAA
TARGET=404,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1198
PRIMER_LEFT_PENALTY=0.095538
PRIMER_RIGHT_PENALTY=0.024252
PRIMER_LEFT_SEQUENCE=CGTCTTCTTCGTATTGCATGAG
PRIMER_RIGHT_SEQUENCE=AGTAGGGACGGTAACGGGTAAT
PRIMER_LEFT=307,22
PRIMER_RIGHT=477,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=60.024
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=0.1394
PRIMER_LEFT_1_PENALTY=0.115189
PRIMER_RIGHT_1_PENALTY=0.024252
PRIMER_LEFT_1_SEQUENCE=GGATCTGAGACGTCTTCTTCGT
PRIMER_RIGHT_1_SEQUENCE=AGTAGGGACGGTAACGGGTAAT
PRIMER_LEFT_1=297,22
PRIMER_RIGHT_1=477,22
PRIMER_LEFT_1_TM=59.885
PRIMER_RIGHT_1_TM=60.024
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=0.1634
PRIMER_LEFT_2_PENALTY=0.139129
PRIMER_RIGHT_2_PENALTY=0.024252
PRIMER_LEFT_2_SEQUENCE=CCATATCCACGTCAACTTTTCA
PRIMER_RIGHT_2_SEQUENCE=AGTAGGGACGGTAACGGGTAAT
PRIMER_LEFT_2=364,22
PRIMER_RIGHT_2=477,22
PRIMER_LEFT_2_TM=59.861
PRIMER_RIGHT_2_TM=60.024
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=0.2497
PRIMER_LEFT_3_PENALTY=0.225403
PRIMER_RIGHT_3_PENALTY=0.024252
PRIMER_LEFT_3_SEQUENCE=ACGTCTTCTTCGTATTGCATGA
PRIMER_RIGHT_3_SEQUENCE=AGTAGGGACGGTAACGGGTAAT
PRIMER_LEFT_3=306,22
PRIMER_RIGHT_3=477,22
PRIMER_LEFT_3_TM=59.775
PRIMER_RIGHT_3_TM=60.024
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.2673
PRIMER_LEFT_4_PENALTY=0.075027
PRIMER_RIGHT_4_PENALTY=0.192236
PRIMER_LEFT_4_SEQUENCE=TTTCCGGGTTACAGGTCATATC
PRIMER_RIGHT_4_SEQUENCE=GAAAAATAGGGAGTAGGGACGG
PRIMER_LEFT_4=343,22
PRIMER_RIGHT_4=488,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=60.192
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.5000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_03736 Ps_contig_mira-and-tgicl-ass_franssen_30904
SEQUENCE=ATGGNGAAAGGTAGGATGATTCTCAGGAGAGGGCGGAAAGTACTCCTTTGATTCGAAGAATGAACAAACGAAGGGTTTGGTAAGAAAGGAAGGTGACGATTGGTTGTCATTGGAACACAGAGACTTCGTACCCGACCCGAATATGGTTCCTAAACAGATCCGGATCGTTGATTTCAAGACAGGTTGCGAAGTTTACGCCGGATCCGCGTTCGCGATGTCTCCGTCACCGAACGCACTTCCGATTCCGTCTTTTCAGAGGAAGTTTGCGCCGGTGGCCTTCGACGACTCCGCAACACGGGATCTGAGACGTCTTCTTCGTATTGCATGAGTCTGAAACGAGTCATTTCCGGGTTACAGGTCATATCCATATCCACGTCAACTTTTCATCTTCTCTTCCTTTTTGTCTCTCTCTCTCTCCCTCTCTCTCTCTTTCTCTCCTTAATCTAGAATCTTCTTATTACCCGTTACCGTCCCTACTCCCTATTTTTCTAATTCTACTCACCGTGTATTTTTTAAACGGTAAGGGTGAATTAAAAATAGGGTAAACGTCGGGGCGGAAATGTTAAATTAAAAAAGTCTGGAGTAGTGTGGTTGAGTAGGTTTTATGAAGATCTTTGTTTAAATTAA
TARGET=419,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1198
PRIMER_LEFT_PENALTY=0.095538
PRIMER_RIGHT_PENALTY=0.024252
PRIMER_LEFT_SEQUENCE=CGTCTTCTTCGTATTGCATGAG
PRIMER_RIGHT_SEQUENCE=AGTAGGGACGGTAACGGGTAAT
PRIMER_LEFT=307,22
PRIMER_RIGHT=477,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=60.024
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=0.1394
PRIMER_LEFT_1_PENALTY=0.115189
PRIMER_RIGHT_1_PENALTY=0.024252
PRIMER_LEFT_1_SEQUENCE=GGATCTGAGACGTCTTCTTCGT
PRIMER_RIGHT_1_SEQUENCE=AGTAGGGACGGTAACGGGTAAT
PRIMER_LEFT_1=297,22
PRIMER_RIGHT_1=477,22
PRIMER_LEFT_1_TM=59.885
PRIMER_RIGHT_1_TM=60.024
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=0.1634
PRIMER_LEFT_2_PENALTY=0.139129
PRIMER_RIGHT_2_PENALTY=0.024252
PRIMER_LEFT_2_SEQUENCE=CCATATCCACGTCAACTTTTCA
PRIMER_RIGHT_2_SEQUENCE=AGTAGGGACGGTAACGGGTAAT
PRIMER_LEFT_2=364,22
PRIMER_RIGHT_2=477,22
PRIMER_LEFT_2_TM=59.861
PRIMER_RIGHT_2_TM=60.024
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=0.2497
PRIMER_LEFT_3_PENALTY=0.225403
PRIMER_RIGHT_3_PENALTY=0.024252
PRIMER_LEFT_3_SEQUENCE=ACGTCTTCTTCGTATTGCATGA
PRIMER_RIGHT_3_SEQUENCE=AGTAGGGACGGTAACGGGTAAT
PRIMER_LEFT_3=306,22
PRIMER_RIGHT_3=477,22
PRIMER_LEFT_3_TM=59.775
PRIMER_RIGHT_3_TM=60.024
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.2673
PRIMER_LEFT_4_PENALTY=0.075027
PRIMER_RIGHT_4_PENALTY=0.192236
PRIMER_LEFT_4_SEQUENCE=TTTCCGGGTTACAGGTCATATC
PRIMER_RIGHT_4_SEQUENCE=GAAAAATAGGGAGTAGGGACGG
PRIMER_LEFT_4=343,22
PRIMER_RIGHT_4=488,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=60.192
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.5000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_03737 Ps_contig_mira-and-tgicl-ass_franssen_30909
SEQUENCE=TGAACNATGTTTAGGATATTTTGTTTATTAGTATCACTATATTATAATCTTCAATGGATCTCAAGTTCACCCCTTTCTCTGCAACTCTCACAATTTCATGGTTTTCAACTAATAATAAGTTTTGAGCAAAACTTTTGAGAATGAGATTTGTGGAAGACAAGGGATGCTATAGTAGTAACCATGGATCAGTAGTACAAGATACTCATAAAGGAAAAGATGTTACTACTACTAGTACTACTGCTACTGCTGCTGCTGCTAGTGCTCGTGTTTCGAATCATCATCGAATAAACATGGGAAAACCGACTCCGTCTAAATGGGACGACGCGCAAAAATGGCTAGTTGGTTTATCAAAAGGCGGAGAGAAAAGCCAATCAAAAAAGCAAGCCTAGAAACTCAAATGCAGATGATTTGAGACTAATAGCTCCCGTGCCGCAGAAAGAGGAAGAACACGAGGATGACGAGGATGGATGCCGTGAGTTCATGACAACGACTAGTACTGAATATGTTGAAGAAGAAACCAAGAAAGTAGAATGTGATGAATCAATTTGGAGAATGAATAACAAGGACACAATCCATGTTCAATCAATATGTTTTCGAGACATGGGGACTGAGATGACACCTATTGCTAGTCAAGAGCCTTCAAGAACTACGACACCTATTAGAGCAACAACCCCTAATACTAGAAGTCCAATTCATTCAGGTACTTCAACTCCAATGAGGAATCAAAATGGATTCTTGCAAATGGAAGCTGCATCAGGTAATGGTAGCACAGCAACTAGACAATGTGGAGAAGGATCAAGTCCTTGTAATATGGTTGAAAAGAATATGAAGATTGATGATCAAGCTAGAAAGATGGGGCCTCTAGAATGTAGAGCAATGGCTTGGGATGAAGCCGAACGCGCCAAATATACGGCTAGGTTTAAGCGCGAAGAGATGAAGATTCAAGCATGGGAGAATCATCAGATAAGAAAAGCTGAAATGGAAATGAAGAAAATGGAGGTGAGAGCTGAAAGAATGAAAGCTGTGGCACAAGAAAAGTTAACAAATAAAATGGCTGCAACAAGGAGAATAGCTGAAGAAAAGAGAGCTAATGCACAAGTGAAACTGAATGACAATGCTTTGAAGACCACTGAGAAAGTAGAGTATATAAGAAGGACAGGATATGTGCCTTCTTCATTCTCTTTCACTTTTAAGTTGCCTTCCATTTGTTGGTAGCAAGTACTAGAACTTCATATGTGTAATTTCTTTTTTTTGTCTTTAAACAAATGTTTAAAGTGGGAATAACCACTAAAAAGTCACACATCCGTGAATAGTAGTAGGGTTCGAACCCTGAGAATGATGTAAAACCTAACGATATTAGTTTCTACTGGTTGAGTTAGGATTTGCCAACTGATATGTGTAATTTATATGTTGTATGAAATAGTAAACCATTTGAAA
TARGET=244,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2740
PRIMER_LEFT_PENALTY=0.273230
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=CTCAAGTTCACCCCTTTCTCTG
PRIMER_RIGHT_SEQUENCE=CATTTAGACGGAGTCGGTTTTC
PRIMER_LEFT=59,22
PRIMER_RIGHT=315,22
PRIMER_LEFT_TM=60.273
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.3444
PRIMER_LEFT_1_PENALTY=0.343680
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=CCTTTCTCTGCAACTCTCACAA
PRIMER_RIGHT_1_SEQUENCE=CATTTAGACGGAGTCGGTTTTC
PRIMER_LEFT_1=71,22
PRIMER_RIGHT_1=315,22
PRIMER_LEFT_1_TM=59.656
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.4764
PRIMER_LEFT_2_PENALTY=0.475706
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=GAGATTTGTGGAAGACAAGGGA
PRIMER_RIGHT_2_SEQUENCE=CATTTAGACGGAGTCGGTTTTC
PRIMER_LEFT_2=142,22
PRIMER_RIGHT_2=315,22
PRIMER_LEFT_2_TM=60.476
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.4986
PRIMER_LEFT_3_PENALTY=0.273230
PRIMER_RIGHT_3_PENALTY=0.225403
PRIMER_LEFT_3_SEQUENCE=CTCAAGTTCACCCCTTTCTCTG
PRIMER_RIGHT_3_SEQUENCE=ATGATTCGAAACACGAGCACTA
PRIMER_LEFT_3=59,22
PRIMER_RIGHT_3=277,22
PRIMER_LEFT_3_TM=60.273
PRIMER_RIGHT_3_TM=59.775
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=219
PRIMER_PAIR_PENALTY_4=0.5691
PRIMER_LEFT_4_PENALTY=0.343680
PRIMER_RIGHT_4_PENALTY=0.225403
PRIMER_LEFT_4_SEQUENCE=CCTTTCTCTGCAACTCTCACAA
PRIMER_RIGHT_4_SEQUENCE=ATGATTCGAAACACGAGCACTA
PRIMER_LEFT_4=71,22
PRIMER_RIGHT_4=277,22
PRIMER_LEFT_4_TM=59.656
PRIMER_RIGHT_4_TM=59.775
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_03738 Ps_contig_mira-and-tgicl-ass_franssen_30910
SEQUENCE=AAATAAAAAAAGGAAATTGAAATTGAAAATCGGTNGRRGAGAAGAGAAGAGAATGCCGAAGAGGACGACGCACACATACTCAAGCGAGGATGCGGCTCCCGAAGGTCCTAACTCCGATCTGTTTGTTTATTACTGCAAACACTGCGGTTCCCATGTCCTCATCACTGACACCCAATTGCAGAAAATGCCTAGAAGAAACACCGACAGAGCTTACGTTTTAGACAAGACCAAACATCTTGCTAGGTTCAACATTGACGAAGCTGGCAACGTTCTCTTAAAGCGAGGGGAAGGAAAAGTCGAGAAACAGTTCCGTATGAATTGTATCGGTTGCGGTCTCTTTGTCTGCTATCGTTCTCAACAAGATTTGGATTCTTCCACTCTTATTTATGTTCTTGACAAAGCACTCAGTACTGTCGCTGCTGAAACCAACCCGCAGGATGCACCTGTTCCACCTTGTATTTCTCACCTTGAAGGCGGTCTTGTTCAACTTGCCATCGAAGTCGAAGATCGTGCTCACCGCTCCGCCATCACAAGAGTAAATGCCGATGATGTTCGAGTCTCTGTAGCTGCTCCCGCTGCACGTGGAGAAGCTAACAATGAACTTTTGGAGTTTATGGGAAAAGTTCTGGGTTTAAGGTTGAGTCAAATGACTCTTCAGAGAGGATGGAATAATAAGTCAAAGCTTCTTGTGGTGGAGGATTTGACTGCTAGACAAGTTTATGAGAAACTTTTGGAGGCTGTGCAACCTTGATGCTGTTTCTGTGCACGTTTGTATCTCTTTTATCAACAAAAACAAGTTTCGGGTTTGAATTTCAGCTGTATTTATTTAATTTTAGAACGCCATTGGTCTATTGATGTCGGAAATCTAGAGTAAAACTCGTCTGGTTATTCAGCAATTGGGTGGGGTTGGATTGAAACATARCGAGATATGCTTTGAATATGAAAACTGTTAGAAGTCTACACTGTGATTGAAATTTCAAAGACTAGTTAAAAAGAGTGAGACAAAATTATTCTGGTGGTTGTGGAGGAAGAGAGTTAATGATGAAGG
TARGET=39,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03739 Ps_contig_mira-and-tgicl-ass_franssen_30911
SEQUENCE=AAGGGACGTGATAAAAGACAAGATCCATCAACAATAAACAATAGGTCGGATAATAAGGGCNAATACGGCATTGCATATAGCTACCAGGAAAGGTAGAACTCAGATTATTAAGTTGATACTTGGGCAGAGTGAAACAGATGGCATGGCAATAAACAAAAGTGGCGAAACAGCATTAGACACAGCTGAGAAAACAGGAAACTCCGAAGTAAAAAGCATTCTAACAGAACACGGTGTTCAAAGTGGAAAATCCATAACAAAACCAAAAACAGCTGCAACCAGAGAATTAAAACAAACCGTTAGTGACATAAAGCATGAAGTCCATCACCAACTAGAACACACTCGCCAAACGACGAAAAAGCGTTCAAGGAATCGCAAAACGTCTCAACAAAATGCACACAGAAGGGCTCAACAACGCAATAAACTCAACAACCGTCGTCGCAGTCCTCATAGCAACAGTTGCATTCGCCGCTATCTTCACCGTCCCAGGCCAATTCGTCGATAATCCGAAAAAAGTTCCTAAAGGAAAGTCGCTCGGCGAAGCAAACATAGCGCCGCAGGCTGCGTTTCTAATCTTCTTTGTGTTCGACTCGTTTGCGCTTTTCATTTCTTTAGCGGTTGTGGTGGTTCAAACATCAATAGTTGTTATAGAAAGCAAAGCAAAGAAGCAAATGATGGCTATTATTAACAAACTAATGTGGTTGGCTTGTGTGCTTATTTCTGTTTCATTTTTGGCACTGTCTTTTGTAGTTGTTGGGAAAGATCACAGGTGGCTTGCAATTGGAGTAACTATAATTGGAACAACTATTATGGCTACTACATTGGGAACCATGTGTTATTGGGTTATTAGGCATCGAATTGAAGCTTCAAATATGAGGAGTATACGTAAATCTTCAATGGGAAGCAGGTCAAGGTCTTTTTCAGTTTCAGTTGTTATGTCAGATTCTGAGATATTAAACAATGAGACGTAACTAAATGTATGTACTTAATTTAAAGTAAAATTTAATTAATTAAAATTTAAAACTTTTACTAACTTTAAC
TARGET=1000,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03740 Ps_contig_mira-and-tgicl-ass_franssen_30914
SEQUENCE=CTTAAAAATAATGAARANGGTAAATTGTTGTAATTGGAATTTCGAACAAAAAAAACAATTCAAGCTTTGACGACAGCTCATAGCAGTGGTTGACGGCAAACGGAGACACGAACAACACAACACCAAAATAAAAACACTATCTTTTTTCTTTTTCTTTTTCTTTTCTCTTTCTGTCTTCGAAATCATTCATTCATTTTTGCAATTTCCGGTGAGGCCAACGGTGAAACATGGCCGCGTCTGGTGAGGATGATGCCTCCAAACAAGACCTCTTTCACCTCATCAAACGCTTCGGAGCTTACGTTTCTTTCAAGATCTCCGATCTCTTCTCTCTCTCTTTCCATAACCTCGATATACGCACTGTTGGGGCGGTTGCTGGTCTTGCTGTTGCAATTGTTTTCACATGGAGGCTGTTGAGATCTCCCAGTGATAATCAAGGGAGGCAAAGAAAACGGCAGGGTGCTACATCTAGTAATCCTGGAGTCAGCACACATTCTAATACATCGGTTGTTCCTTCTGATGCTTGTTTACCTTCAGATGAATCAAGGGCGCAAAATGTCGTCGATGAGTTCTTTCAACCTGTCAAGCCAACCTTGGGGCAGATAGTGAGGCAGAAGTTGAGTGAAGGAAGAAAGGTAACATGCCGTCTTCTTGGAGTAATCCTTGAGGAAAGCAGTCCAGAGGAGCTTCAGAAACAAGCAACCGTGAGATCCTCTGTGCTAGAAGTGTTATTGGAAATAACTAAATTCTGTGATTTGTATCTCATGGAACGGGTTCTGGATGACGAAAGTGAGAAAAGAGTTCTGGTCGCGTTAGAAGAAGCAGGGGTATTCACTTCAGGTGGTTTGGTCAAGGACAAGGTTCTTTTCAGCAGCATTGAGAACGGACGATCGTCCTTTGTTCGGCAATTGGAACCAGATTGGCATATCGATACAAACTCTGAAATCATAACTCAGTTAGCTAGGTTTATCAAGTATCAGCTCCACATATCGCCGTATAAAACAGAACGAACCGCAGCTAATGTGTTCAGCGCTCCAACCTTGGAACTCTTCTTCGGATCCGCATGAAAACCAAATATCACTACTAATCACATAAATGTGTCTACTGGTTTGGTTTCTGTCTGCATCTTGGATCCAACCTTCAACCATACGTTTTTTCTAGATGGATGAGAAAACTAGGTTAGATTTTCTTCAAGCTTTAATGGGTTCTCACCCTAGGTTGGTGAAAGACCCGTTCCTGTGCATGTGTATGATGCCTTGAGGAAGAAGTTTATATTTGTTTTCTTTGTTTACATTTTGTTTGAAATTTGTGCATTAGATTCAGACATTTTGGACCAACAAAGTAGCAATTTATATTAGATGTTTGTATTCTATGTATTTAACATTGATAATTTGTCATCATGAACATTATTACCTTAAATTCCACAAAACCAACAAGTGGTATTATTGCCA
TARGET=183,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03741 Ps_contig_mira-and-tgicl-ass_franssen_30914
SEQUENCE=CTTAAAAATAATGAARANGGTAAATTGTTGTAATTGGAATTTCGAACAAAAAAAACAATTCAAGCTTTGACGACAGCTCATAGCAGTGGTTGACGGCAAACGGAGACACGAACAACACAACACCAAAATAAAAACACTATCTTTTTTCTTTTTCTTTTTCTTTTCTCTTTCTGTCTTCGAAATCATTCATTCATTTTTGCAATTTCCGGTGAGGCCAACGGTGAAACATGGCCGCGTCTGGTGAGGATGATGCCTCCAAACAAGACCTCTTTCACCTCATCAAACGCTTCGGAGCTTACGTTTCTTTCAAGATCTCCGATCTCTTCTCTCTCTCTTTCCATAACCTCGATATACGCACTGTTGGGGCGGTTGCTGGTCTTGCTGTTGCAATTGTTTTCACATGGAGGCTGTTGAGATCTCCCAGTGATAATCAAGGGAGGCAAAGAAAACGGCAGGGTGCTACATCTAGTAATCCTGGAGTCAGCACACATTCTAATACATCGGTTGTTCCTTCTGATGCTTGTTTACCTTCAGATGAATCAAGGGCGCAAAATGTCGTCGATGAGTTCTTTCAACCTGTCAAGCCAACCTTGGGGCAGATAGTGAGGCAGAAGTTGAGTGAAGGAAGAAAGGTAACATGCCGTCTTCTTGGAGTAATCCTTGAGGAAAGCAGTCCAGAGGAGCTTCAGAAACAAGCAACCGTGAGATCCTCTGTGCTAGAAGTGTTATTGGAAATAACTAAATTCTGTGATTTGTATCTCATGGAACGGGTTCTGGATGACGAAAGTGAGAAAAGAGTTCTGGTCGCGTTAGAAGAAGCAGGGGTATTCACTTCAGGTGGTTTGGTCAAGGACAAGGTTCTTTTCAGCAGCATTGAGAACGGACGATCGTCCTTTGTTCGGCAATTGGAACCAGATTGGCATATCGATACAAACTCTGAAATCATAACTCAGTTAGCTAGGTTTATCAAGTATCAGCTCCACATATCGCCGTATAAAACAGAACGAACCGCAGCTAATGTGTTCAGCGCTCCAACCTTGGAACTCTTCTTCGGATCCGCATGAAAACCAAATATCACTACTAATCACATAAATGTGTCTACTGGTTTGGTTTCTGTCTGCATCTTGGATCCAACCTTCAACCATACGTTTTTTCTAGATGGATGAGAAAACTAGGTTAGATTTTCTTCAAGCTTTAATGGGTTCTCACCCTAGGTTGGTGAAAGACCCGTTCCTGTGCATGTGTATGATGCCTTGAGGAAGAAGTTTATATTTGTTTTCTTTGTTTACATTTTGTTTGAAATTTGTGCATTAGATTCAGACATTTTGGACCAACAAAGTAGCAATTTATATTAGATGTTTGTATTCTATGTATTTAACATTGATAATTTGTCATCATGAACATTATTACCTTAAATTCCACAAAACCAACAAGTGGTATTATTGCCA
TARGET=325,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03742 Ps_contig_mira-and-tgicl-ass_franssen_30921
SEQUENCE=CCTATTAATAAATATCGAAGTTTATCGCTCCCAACCYCRRRGACGCTTTCTTTTCTCTTCTCCGTTAGGGTTTTCTCCACCCTCAGGGTTAGGGTTATATTCTCCCTTTTCTTTCCCCAAATCAATTTCAAAACAACAAATTTCTCTCTTTTCTCACCCAATCACACAACAAAAGCGAAACCAAATCGAAGATGACGGGAAAAGCGAAGCCAAAGAAGCACACGGCGAAGGAGATCCAAGCCAAGGTGGACGCCGCGCTGACCAACCGCGGCGGAGGAAAAGCCGGTTTGCTTGATCGGATCGGTGTTGAGAAGGGAGGACACGCCAAGTGGGAGTGCCCTCACTGCAAAACAACGGTGCCTGATGTGAAATCGATGCAGATTCATCACGATGCTCGTCACCCGAAGATCCCCTTTGAGGAAGATAAGCTCGTGAATCGTCACGCATCTGCAGCTGCTGTTCCGGAATCTTCCAAGCCTCGCCCTGGTGTTCGTGGTAGCCTCAAAAAGTGATCGTTATCATCATCATCATACAAATCGCACGTCCTTTCTATTCTAYGGTTCTTTTACTTATTATTTTTCTTTATTTTTTACGTTTTACCCTTTTTTTCTTACTAACGTTCGTTTCGGTTTTTAGGGTTGTTATTGTAA
TARGET=518,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03743 Ps_contig_mira-and-tgicl-ass_franssen_30922
SEQUENCE=AACTCGCCAAAAATCTGATNNACTTCAATTCAACAAACGCATCTCTCTCTCTCTCTCTCTCTTCAATGGCGGATCACAACCCAAAAAGCTGAGATCTTTGAACTCAACAATGGAACCGTGCAGCTTCTCGTTACCAATCTCGGTTGCACAATCACATCCTTCTCCGTACCTGGCAAAGATGGTGTGTTATCCGATGTTGTTCTTGGTCTGGACTCTGTTGAATCTTATCAGAAAGGTCTTGCTCCTTATTTTGGCTGCATTGTGGGTCGGGTTGCAAACCGAATTAAGGATGGAAAGTTTAAACTTGATGGGGTTGAGTACTCTTTGCCTCTCAACAGACCCCCATGCAGTCTTCATGGTGGAAATGTTGGGTTTGATAAGAAGGTATGGGAGGTAATTGAATATAAAAAAGGAGAAACTCCTTCAATCACTTTTAAGTATGAAAGTCATGATGGAGAGGAAGGTTATCCTGGAGACATCACTGTTACCGCAACTTACACCCTCACCTCAAGCACGACTCTGAGGCTCGACATGGAAGGAGTGCCTAAGAACAAGCCCACAATCATTAACTTAGCTCAGCATACCTATTGGAACTTAGCTGGCCATAACTCAGGCCATATACTTGATCATTCAATTAAGATACCGGCTAACCATGTCACTCCTGTTGATCAGAACACAGTACCAACCGGCGAAATCATGCCAGTGAAGGGGACACCTTTTGATTTTACGTCCGAAAGAAGAATAGGCGACACCGTTAATCAGGTTGGTCTAGGATATGACCATAACTACGTCCTCGATTGTGGCGAAGAGAAAGCTGGTTTGAAGCATGCTGCCAAAGTTAGAGATCCATCGAGCTCAAGGGTACTTAACTTGTGGACGAATGCACCCGGTATGCAATTCTACACAGCAAATTACGTCAACAACGTAACTGGAAAAGGAGGCGCTGTATATGGAAAGCATGCTGGATTATGTCTCGAGACACAAGGATTCCCTGATGCGGTAAACCACTCGCATTTCCCGTCTGTTGTGGTTCGACCTGGCGACAAATATCAACATACTATGTTGTTTGAGTTTTCAATCGAATGAATATTGTTGGAGAGTTTGAGTGAGTACTAAAATAAATAAGGTAGACTTGGTTCTGCTTATTATTATTGTTTAGAAATCGAACTTTGGTTGCTAATAAAACTTTTACTTGTTTTTAAATAAGTGTCTGAAGTCTTTGATAGACTGAGTTTTAAGAG
TARGET=42,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03744 Ps_contig_mira-and-tgicl-ass_franssen_30928
SEQUENCE=TATTTAAACTTAATGTTGCATCATCAAATTATTACCATGTAGTAGAACAATACACTACATTAAATCTTCAATTTACAGAGACAAACTAAAATTACTCATTCCAATTCTAACAACATAAAATTGGTTTAAGTTATTTAAAAATGTCACTCAACTTTAAATATGCATACAAAAACATTGTCCTGCATAAATATTAATGTTAACTACTAAAGAACAATAATAGCTTTCCTCGAGAAAGTTGTAAACAAGATTTTCACTTCATTGCTCGTTCCATAATAGATGAATGCGAAGTAGGAAACATACATTTCTGATTGTCCTATCATCAACAGAACCTAACCTTGATTAAAAGGGTTTATTCTGAATCTCCATAACACTGGGAAGAGGCGGAACATAAAGAGAGTAAACAACGGCCTGTCGTTTAGCTACTTCAAACTGAGATTTTCCGAACGAGAAAGCTTCGGAAACCGAACAAGATTCAGAAAGTGTTGCGTTTTGACGAAACGCATCGACGGCACGTCGTTTAGTGTATTCCCTAATGTTGTAATCTGGAAATTGACGAGATACTCGTATGAAGGAACGGAGGAGCGATAGGATTTGGGATTTTGAAGGAGATGAAGCCGCCGCCGCCGCCGCCATGGTAACCGCCACGCAGAGGAAAAGTAGAATCGGCGATAAAAAATAGGTGGAATCAAGAAACGAGATTGAAGAGGAACTGAATCGGTGACGATGCTCGAATGCGGAGAATCGTGGTGGGTCTGATGAGTGTAGTTTTAANCCGGTAA
TARGET=614,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2675
PRIMER_LEFT_PENALTY=0.180440
PRIMER_RIGHT_PENALTY=0.087031
PRIMER_LEFT_SEQUENCE=AAAGAGAGTAAACAACGGCCTG
PRIMER_RIGHT_SEQUENCE=CGATTCTACTTTTCCTCTGCGT
PRIMER_LEFT=389,22
PRIMER_RIGHT=664,22
PRIMER_LEFT_TM=59.820
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.2706
PRIMER_LEFT_1_PENALTY=0.183585
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=AGAGAGTAAACAACGGCCTGTC
PRIMER_RIGHT_1_SEQUENCE=CGATTCTACTTTTCCTCTGCGT
PRIMER_LEFT_1=391,22
PRIMER_RIGHT_1=664,22
PRIMER_LEFT_1_TM=59.816
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=274
PRIMER_PAIR_PENALTY_2=0.3122
PRIMER_LEFT_2_PENALTY=0.237699
PRIMER_RIGHT_2_PENALTY=0.074466
PRIMER_LEFT_2_SEQUENCE=AAACTGAGATTTTCCGAACGAG
PRIMER_RIGHT_2_SEQUENCE=CCGATTCAGTTCCTCTTCAATC
PRIMER_LEFT_2=426,22
PRIMER_RIGHT_2=717,22
PRIMER_LEFT_2_TM=59.762
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=292
PRIMER_PAIR_PENALTY_3=0.3160
PRIMER_LEFT_3_PENALTY=0.241544
PRIMER_RIGHT_3_PENALTY=0.074466
PRIMER_LEFT_3_SEQUENCE=AACTGAGATTTTCCGAACGAGA
PRIMER_RIGHT_3_SEQUENCE=CCGATTCAGTTCCTCTTCAATC
PRIMER_LEFT_3=427,22
PRIMER_RIGHT_3=717,22
PRIMER_LEFT_3_TM=60.242
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.3247
PRIMER_LEFT_4_PENALTY=0.237699
PRIMER_RIGHT_4_PENALTY=0.087031
PRIMER_LEFT_4_SEQUENCE=AAACTGAGATTTTCCGAACGAG
PRIMER_RIGHT_4_SEQUENCE=CGATTCTACTTTTCCTCTGCGT
PRIMER_LEFT_4=426,22
PRIMER_RIGHT_4=664,22
PRIMER_LEFT_4_TM=59.762
PRIMER_RIGHT_4_TM=59.913
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_03745 Ps_contig_mira-and-tgicl-ass_franssen_30930
SEQUENCE=CATAAACAAAAACTATTCGTYCCTACCAAAAATTAATTAATGTTAACTACTAAAGAGCAATAATAGCTTTCCTCGAGAAAGTTGTAAACAAGATTTTCACTTCATTGCTCGTTCCATAATAGATGAATGCGAAGTAGGGAAACACATTTCTGATTGTCCTATCATCAACATAACATAACCTTGATTAAAAGGGTTTATTCTGAATCTCCATAACACTGGGAAGAGGCGGAACATAAAGAGAGTAAACAACGGCCTGTCGTTTAGCTACTTCAAACTGAGATTTTCCGAACGAGAAAGCTTCGGAAACCGAACAAGATTCAGAAAGTGTTGCGTTTTGACGAAACGCATCGACGGCACGTCGTTTAGTGTATTCCCTAATGTTGTAATCTGGAAATTGACGAGATACTCGTATGAAGGAACGGAGGAGCGATAGGATTTGGGATTTTGAAGGAGATAAAGTTGCCGCCGCCGCCGCCGCCGCCATGGTAACCGCCACGCAGAGGAAAAGCAGAACCGGCGATGAAAAATCGGTGGAATCAAGAAACGAGATTGAAGATCATAAATATGTTGTATATGTGTAATAGTTTGCATATATTCAAAAAAAATGTTACATACCAAAATCTCTTATTCTTTTATATATTGTGTAATTGAATTTGCTTTGCTTAGTTTGATGCATGTGAATGTGTATTGTGAATCTCTCATTTTGTAATTTTCATTTTTGTCATTTTAATGTCAAAGAATTGTGACTTTGGATTGATAAC
TARGET=462,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03746 Ps_contig_mira-and-tgicl-ass_franssen_30931
SEQUENCE=CGTAGAAATAACAAAGAAGTCATTTCCGTTTTCACTTGATTTTCGGTGCGATGCGTGTGAGCAACTCAGTGATAAATACTAAATAGTTCTTTCTTTCTTTCTTCTCTTCTCTTCTTCAGAATACAACACAACAAAACAGTTCTCAATTTTCCAATGTGAAGAAAAAAAGAGCAATCAAAATGGCCGTTATGAGTGCTTTCAGGACTCTATCTTCCAAATCGAGGTCACTCACGACGGCACTCGTTAAATCATGCCGGTCGGCGTCGTACTCCTCCGCGGCGGTGGTGGCTGATGACAGTTTTGAGAGCGGCACGTATTTGAAAGATTATGCTGATTATAGAAGATCTCTGTACGGTCAGATTACGCATAAAGCTCTTCTCGTTGATGCCGTTGGTACTCTTGTTCTTCCTTCTCAACCTATGGCTCAGATATATAGAAAAATTGGGGAGAAATATGGTGTGGAGTATTCAGAGGAAGAAATTTTGCATAGATACAGAAGAGCTTATAGTCAACCTTGGGGTAAATCTCGTCTCAGATATGTTAAAGATGGTAGACCGTTCTGGCAATACATTGTTAGTAATTCTACCGGCTGTGATGATTCTCAATACTTTGAAGAACTTTATAACTACTATGTCACAGACAAGGCATGGCACCTATGTGATCCTAATGCAGGAGAAGTGTTCAAAGCTCTTAGAAAATCAGGTGTAAAATTGGCTGTTGTGTCAAATTTTGACACCCGGTTAAGACCTCTTCTGCGGGCATTAAACTGTGATGATTGGTTTGATGCTGTAGCAGTATCAGCTGAGGTTGCAGCCGAGAAACCAAATCCAACTATATTTCTCAAAGCGTGTGAACTATTGGACGTAAAACCGGAGGATGCTGTTCATGTCGGGGATGACCGCCGCAATGATGTATGGGGTGCTAGAGATGCAGGCTGTGATGCTTGGCTTTGGGGAAGTGATGTTCACTCTTTTAAAGAGGTAGCACAGAGGATCGGGGTCCAGGTCTGAAAAAGTATGTTCTATGCAATAAGTTGAAGAATGTTATGTATATAAATAGGATTATGTTAGTAGTCTTTTTAGGCTTTAGTTACAAGGATGGACTGAAAGAAATGTGAGACTGTAACTTATGAAACAGAAATGAAACAGAAATGAAGGTGTAGCTAGGAGCTTTTATCTTTCCATTCATTAATGTGTTCTCTAAAGTTATAAAAAGATTATTAATGAACTAAAAATAAAACGGTGGAATAATTTGAGC
TARGET=87,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1669
PRIMER_LEFT_PENALTY=0.867981
PRIMER_RIGHT_PENALTY=0.298898
PRIMER_LEFT_SEQUENCE=GTTTTCACTTGATTTTCGGTGC
PRIMER_RIGHT_SEQUENCE=TCTCAAAACTGTCATCAGCCAC
PRIMER_LEFT=27,22
PRIMER_RIGHT=305,22
PRIMER_LEFT_TM=60.868
PRIMER_RIGHT_TM=60.299
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=1.2867
PRIMER_LEFT_1_PENALTY=0.867981
PRIMER_RIGHT_1_PENALTY=0.418705
PRIMER_LEFT_1_SEQUENCE=GTTTTCACTTGATTTTCGGTGC
PRIMER_RIGHT_1_SEQUENCE=GAGTGACCTCGATTTGGAAGAT
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=229,22
PRIMER_LEFT_1_TM=60.868
PRIMER_RIGHT_1_TM=59.581
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=1.2988
PRIMER_LEFT_2_PENALTY=0.999901
PRIMER_RIGHT_2_PENALTY=0.298898
PRIMER_LEFT_2_SEQUENCE=CGTGTGAGCAACTCAGTGATAA
PRIMER_RIGHT_2_SEQUENCE=TCTCAAAACTGTCATCAGCCAC
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=305,22
PRIMER_LEFT_2_TM=59.000
PRIMER_RIGHT_2_TM=60.299
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=1.2995
PRIMER_LEFT_3_PENALTY=0.867981
PRIMER_RIGHT_3_PENALTY=0.431486
PRIMER_LEFT_3_SEQUENCE=GTTTTCACTTGATTTTCGGTGC
PRIMER_RIGHT_3_SEQUENCE=CTCTCAAAACTGTCATCAGCCA
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=306,22
PRIMER_LEFT_3_TM=60.868
PRIMER_RIGHT_3_TM=60.431
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=1.4186
PRIMER_LEFT_4_PENALTY=0.999901
PRIMER_RIGHT_4_PENALTY=0.418705
PRIMER_LEFT_4_SEQUENCE=CGTGTGAGCAACTCAGTGATAA
PRIMER_RIGHT_4_SEQUENCE=GAGTGACCTCGATTTGGAAGAT
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=229,22
PRIMER_LEFT_4_TM=59.000
PRIMER_RIGHT_4_TM=59.581
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_03747 Ps_contig_mira-and-tgicl-ass_franssen_30931
SEQUENCE=CGTAGAAATAACAAAGAAGTCATTTCCGTTTTCACTTGATTTTCGGTGCGATGCGTGTGAGCAACTCAGTGATAAATACTAAATAGTTCTTTCTTTCTTTCTTCTCTTCTCTTCTTCAGAATACAACACAACAAAACAGTTCTCAATTTTCCAATGTGAAGAAAAAAAGAGCAATCAAAATGGCCGTTATGAGTGCTTTCAGGACTCTATCTTCCAAATCGAGGTCACTCACGACGGCACTCGTTAAATCATGCCGGTCGGCGTCGTACTCCTCCGCGGCGGTGGTGGCTGATGACAGTTTTGAGAGCGGCACGTATTTGAAAGATTATGCTGATTATAGAAGATCTCTGTACGGTCAGATTACGCATAAAGCTCTTCTCGTTGATGCCGTTGGTACTCTTGTTCTTCCTTCTCAACCTATGGCTCAGATATATAGAAAAATTGGGGAGAAATATGGTGTGGAGTATTCAGAGGAAGAAATTTTGCATAGATACAGAAGAGCTTATAGTCAACCTTGGGGTAAATCTCGTCTCAGATATGTTAAAGATGGTAGACCGTTCTGGCAATACATTGTTAGTAATTCTACCGGCTGTGATGATTCTCAATACTTTGAAGAACTTTATAACTACTATGTCACAGACAAGGCATGGCACCTATGTGATCCTAATGCAGGAGAAGTGTTCAAAGCTCTTAGAAAATCAGGTGTAAAATTGGCTGTTGTGTCAAATTTTGACACCCGGTTAAGACCTCTTCTGCGGGCATTAAACTGTGATGATTGGTTTGATGCTGTAGCAGTATCAGCTGAGGTTGCAGCCGAGAAACCAAATCCAACTATATTTCTCAAAGCGTGTGAACTATTGGACGTAAAACCGGAGGATGCTGTTCATGTCGGGGATGACCGCCGCAATGATGTATGGGGTGCTAGAGATGCAGGCTGTGATGCTTGGCTTTGGGGAAGTGATGTTCACTCTTTTAAAGAGGTAGCACAGAGGATCGGGGTCCAGGTCTGAAAAAGTATGTTCTATGCAATAAGTTGAAGAATGTTATGTATATAAATAGGATTATGTTAGTAGTCTTTTTAGGCTTTAGTTACAAGGATGGACTGAAAGAAATGTGAGACTGTAACTTATGAAACAGAAATGAAACAGAAATGAAGGTGTAGCTAGGAGCTTTTATCTTTCCATTCATTAATGTGTTCTCTAAAGTTATAAAAAGATTATTAATGAACTAAAAATAAAACGGTGGAATAATTTGAGC
TARGET=100,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1669
PRIMER_LEFT_PENALTY=0.867981
PRIMER_RIGHT_PENALTY=0.298898
PRIMER_LEFT_SEQUENCE=GTTTTCACTTGATTTTCGGTGC
PRIMER_RIGHT_SEQUENCE=TCTCAAAACTGTCATCAGCCAC
PRIMER_LEFT=27,22
PRIMER_RIGHT=305,22
PRIMER_LEFT_TM=60.868
PRIMER_RIGHT_TM=60.299
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=1.2867
PRIMER_LEFT_1_PENALTY=0.867981
PRIMER_RIGHT_1_PENALTY=0.418705
PRIMER_LEFT_1_SEQUENCE=GTTTTCACTTGATTTTCGGTGC
PRIMER_RIGHT_1_SEQUENCE=GAGTGACCTCGATTTGGAAGAT
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=229,22
PRIMER_LEFT_1_TM=60.868
PRIMER_RIGHT_1_TM=59.581
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=1.2988
PRIMER_LEFT_2_PENALTY=0.999901
PRIMER_RIGHT_2_PENALTY=0.298898
PRIMER_LEFT_2_SEQUENCE=CGTGTGAGCAACTCAGTGATAA
PRIMER_RIGHT_2_SEQUENCE=TCTCAAAACTGTCATCAGCCAC
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=305,22
PRIMER_LEFT_2_TM=59.000
PRIMER_RIGHT_2_TM=60.299
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=1.2995
PRIMER_LEFT_3_PENALTY=0.867981
PRIMER_RIGHT_3_PENALTY=0.431486
PRIMER_LEFT_3_SEQUENCE=GTTTTCACTTGATTTTCGGTGC
PRIMER_RIGHT_3_SEQUENCE=CTCTCAAAACTGTCATCAGCCA
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=306,22
PRIMER_LEFT_3_TM=60.868
PRIMER_RIGHT_3_TM=60.431
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=1.4186
PRIMER_LEFT_4_PENALTY=0.999901
PRIMER_RIGHT_4_PENALTY=0.418705
PRIMER_LEFT_4_SEQUENCE=CGTGTGAGCAACTCAGTGATAA
PRIMER_RIGHT_4_SEQUENCE=GAGTGACCTCGATTTGGAAGAT
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=229,22
PRIMER_LEFT_4_TM=59.000
PRIMER_RIGHT_4_TM=59.581
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_03748 Ps_contig_mira-and-tgicl-ass_franssen_30934
SEQUENCE=ATGAACCCATTANTYRGGGGAGTGTATTTCTTGATATATTCTCTTCTTCTCGGCCGTCGCCTAGTAGACCGACCAACCCATCTCTCCGGCAGTTGAGCGACCAACCGCCGCACGCCCGTGTTAATTCTCCTCTGAGGATAAAAATGCCAAAAGTAAAGACAAGCCGAGTTAAATACCCAGAAGGCTGGGAGTTAATTGAGCCTACACTTCGCGAACTTCAAGCAAAGATGAGGGAAGCTGAGAATGATCCACACGATGGCAAAAGAAAATGTGAGACTTTGTGGCCTATATTTAAAATTGCACACCAGAAGAGCCGCTACGTATTTGACCTCTACTATAAGAGAAAGGAAATTTCTAAAGAATTGTATGAATTCTGTTTAGATCAAGGATACGCTGACCGCAATCTTATTGCAAAATGGAAGAAGCCTGGTTATGAACGCCTTTGTTGCTTAAGGTGCATCCAGCCACGCGATCACAACTATGCCACGACTTGTGTTTGCAGAGTGCCCAAGCATCTTAGAGAGGAGAAGGTTATAGAGTGTGTTCACTGTGGTTGCAAGGGTTGTGCAAGCGGGGACTGATCGAACCTCGATTCTATTATCCAGAGGGTTTGTTGGAATCATTTGGTTATGTGTTCTGAAACCCTCCCAGGGCTTAACTTCTCCACCTTGTATTACAGTTTACTAACCTCAAAGAGCTATGCACTGGGAAAGTTTTAGTTAATTATTATATCATCAGCATAGTTCAAAACTGACTTGAGTTTTGTAATATTTGGTTTGGAATAGTCGAACAACTTGTAATACTTTTTGCTCCTTGTATTATTATTATTTCTTAGACTTCTTCNTAAAG
TARGET=817,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03749 Ps_contig_mira-and-tgicl-ass_franssen_30935
SEQUENCE=GAGTACGCGGGGGTTTTGCGTTGGTTGTTTAACCACCTCAACACACAAACACACGCCAGACGAACACCACTCGTCTTCACTCCGACCCCTTATTCCCAATCCAATGACTTCTCCCGTGGTTGCGGAAGCTGCCACCGTCGCAGCTTTGCGGTCGGTTATGCTCCGGGTCCAACAAGCAGCAGAAAGATCCGGAACCAAACCCGATCGAGTCCGAGTCGTCGCAGTATCCAAAACCAAACCCATTTCCATGATTCGCCAACTCTACGACGCAGGTCACCGTTGCTTCGGCGAAAACTACGTCCAAGAAATCATTGAAAAAGCTCCTCAGCTTCCACAAGACATTCAGTGGCACTTCATTGGCCATTTGCAAAGCAATAAAGTCAAAGCACTCTTGAGCGGAGTTCCAAATTTGGCCATGGTTGAGGGTGTGGACAATCAGAAGGTGGCAAATAATCTCGACCGTATGGTTTCAACCCTGGGAAGAAATCCTCTGAGAGTTTTGGTGCAAGTAAATACCAGTGGAGAAGAGTCCAAGTCAGGTGTTGATCCTTCTAATTGTGTTGATCTTGCAAAACATGTGAAATTGAGCTGCCCAAACTTAGAATTCTCTGGCTTAATGACAATAGGAATGCTAGATTATACCTCAACTCCGCAGAACTTTCAGACCCTATCAAATTGCAGGACTGAAGTTTGCAAGGCACTTGAAATGGATGAGGAGCAATGTGAACTGTCAATGGGCATGTCCGGTGATTTTGAGTTAGCGATCGAATCGGGCAGTACTAATGTGAGAGTTGGATCAACAATATTTGGACCAAGAGAGTATGCAAAAAAACAATAATAGCAGAAAGATACAATTTCTCTCTTCTTCTGTCTAAAGCTAACATGCTTGCGTTAAGACAGATCTCATGAATGCATGTTGGTTCAAGCTACTTGGAGAGAGAGGGAAAGAGAAGCAAATATAGTATGGTATATAGTCGCATCATGCTCCATTTTAACAGTGGTCATCTGCAAAAAGCAACCAAATAATTGTAACCTAATGTTTGAATAATATTTATCTATAAAATTATTATTATTATTTGCAGGCCAATTTGCACCGTGGTTATTCTTAAGGATCTGGTCTTAAAGAC
TARGET=1063,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4014
PRIMER_LEFT_PENALTY=0.037936
PRIMER_RIGHT_PENALTY=0.363508
PRIMER_LEFT_SEQUENCE=AGATCTCATGAATGCATGTTGG
PRIMER_RIGHT_SEQUENCE=TCCTTAAGAATAACCACGGTGC
PRIMER_LEFT=898,22
PRIMER_RIGHT=1111,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=60.364
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=214
PRIMER_PAIR_PENALTY_1=0.4159
PRIMER_LEFT_1_PENALTY=0.052366
PRIMER_RIGHT_1_PENALTY=0.363508
PRIMER_LEFT_1_SEQUENCE=AACATGCTTGCGTTAAGACAGA
PRIMER_RIGHT_1_SEQUENCE=TCCTTAAGAATAACCACGGTGC
PRIMER_LEFT_1=879,22
PRIMER_RIGHT_1=1111,22
PRIMER_LEFT_1_TM=59.948
PRIMER_RIGHT_1_TM=60.364
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.4590
PRIMER_LEFT_2_PENALTY=0.095522
PRIMER_RIGHT_2_PENALTY=0.363508
PRIMER_LEFT_2_SEQUENCE=GGAGAGAGAGGGAAAGAGAAGC
PRIMER_RIGHT_2_SEQUENCE=TCCTTAAGAATAACCACGGTGC
PRIMER_LEFT_2=932,22
PRIMER_RIGHT_2=1111,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.364
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.5016
PRIMER_LEFT_3_PENALTY=0.138114
PRIMER_RIGHT_3_PENALTY=0.363508
PRIMER_LEFT_3_SEQUENCE=GAATGCATGTTGGTTCAAGCTA
PRIMER_RIGHT_3_SEQUENCE=TCCTTAAGAATAACCACGGTGC
PRIMER_LEFT_3=907,22
PRIMER_RIGHT_3=1111,22
PRIMER_LEFT_3_TM=60.138
PRIMER_RIGHT_3_TM=60.364
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=0.7037
PRIMER_LEFT_4_PENALTY=0.340219
PRIMER_RIGHT_4_PENALTY=0.363508
PRIMER_LEFT_4_SEQUENCE=GCTTGCGTTAAGACAGATCTCA
PRIMER_RIGHT_4_SEQUENCE=TCCTTAAGAATAACCACGGTGC
PRIMER_LEFT_4=884,22
PRIMER_RIGHT_4=1111,22
PRIMER_LEFT_4_TM=59.660
PRIMER_RIGHT_4_TM=60.364
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_03750 Ps_contig_mira-and-tgicl-ass_franssen_30938
SEQUENCE=GGTATCAACGCAGAGGACGGCGGGCGACGTTATTAGTCTTCTCCAGCTCTCTCTCTCTCTCTCTCTCTCTCTAACACAACCGTTTTTCTTTCRCCGGGAAAAGTACAACAACTGATCTTAGTCTCCGCCGATTCTTCTCCGATCTCTCCGCCATGAGTTCCAGCGAGCTCCTCCAGATACACCCTCAGGAGCTACAGTTCCCTTT
TARGET=47,26
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03751 Ps_contig_mira-and-tgicl-ass_franssen_30947
SEQUENCE=CGGAGGAAGAATCACCCTTCCAGAGAAAGCAGTGGTATCAACGCAGAGTGAACAATCGAAAGACTCCAAGTGCATTGATAGAGAGGTTTTACAAAGAGAGCTCAAAGAGGTTTTAGCAAAGCAAGCTGAGTTGGGAGTTGAAGTTGCCGAAATACCTTCATACTATCTGAAGAATGCTGCAAATCAAGGCCTTCAGAGTGAAGAGAATGAAACCCCATTTACCAACAAAAGAAAATTCAAAAACAAGGCAAGAAGGAATCCAAAACAAAAGGCGTCGGAACGGCAAGAAGCAAGAAGCTGGCTGACAAAGATTTTTTAGAAAACAAGAAAGAAAGACCAAACTTTAATTAACAAGAAAACTAACTTGAGTGCAGATATAGTGAGGG
TARGET=325,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9700
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=2.952109
PRIMER_LEFT_SEQUENCE=CTGAAGAATGCTGCAAATCAAG
PRIMER_RIGHT_SEQUENCE=CCCTCACTATATCTGCACTCAAG
PRIMER_LEFT=166,22
PRIMER_RIGHT=385,23
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=58.048
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=3.0066
PRIMER_LEFT_1_PENALTY=0.054514
PRIMER_RIGHT_1_PENALTY=2.952109
PRIMER_LEFT_1_SEQUENCE=GAGAATGAAACCCCATTTACCA
PRIMER_RIGHT_1_SEQUENCE=CCCTCACTATATCTGCACTCAAG
PRIMER_LEFT_1=202,22
PRIMER_RIGHT_1=385,23
PRIMER_LEFT_1_TM=60.055
PRIMER_RIGHT_1_TM=58.048
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=3.0141
PRIMER_LEFT_2_PENALTY=0.017846
PRIMER_RIGHT_2_PENALTY=2.996204
PRIMER_LEFT_2_SEQUENCE=CTGAAGAATGCTGCAAATCAAG
PRIMER_RIGHT_2_SEQUENCE=CCCTCACTATATCTGCACTCAA
PRIMER_LEFT_2=166,22
PRIMER_RIGHT_2=385,22
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=57.004
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=3.0507
PRIMER_LEFT_3_PENALTY=0.054514
PRIMER_RIGHT_3_PENALTY=2.996204
PRIMER_LEFT_3_SEQUENCE=GAGAATGAAACCCCATTTACCA
PRIMER_RIGHT_3_SEQUENCE=CCCTCACTATATCTGCACTCAA
PRIMER_LEFT_3=202,22
PRIMER_RIGHT_3=385,22
PRIMER_LEFT_3_TM=60.055
PRIMER_RIGHT_3_TM=57.004
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=3.1067
PRIMER_LEFT_4_PENALTY=0.017846
PRIMER_RIGHT_4_PENALTY=3.088826
PRIMER_LEFT_4_SEQUENCE=CTGAAGAATGCTGCAAATCAAG
PRIMER_RIGHT_4_SEQUENCE=CCCTCACTATATCTGCACTCAAGTT
PRIMER_LEFT_4=166,22
PRIMER_RIGHT_4=385,25
PRIMER_LEFT_4_TM=60.018
PRIMER_RIGHT_4_TM=60.089
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=44.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_03752 Ps_contig_mira-and-tgicl-ass_franssen_30950
SEQUENCE=ACCAAACCAAGAAACAACCAGATTCCCGTGACACTTTAATTACTGCNCAAAAATAAAAACYNACTCCCAACCAACTTNGTTTATTTGTCATTACGTCTYCCCCACCCCCCACCCACTGCCATCACTTTCTCTCTCTCTCTCTCTCTCAGTTTCTCTCTCTTCTTTCCTGTAGCAGGTCTGTTTTCCGAGCATAGTAAGTAATAATCTGCCAAACTAAGCCTCTGCTACACTCTAGCACCCTACTCTCTCATTTTCACCTATTCAACTAGCAATTATTGTGGTGTTACATAAAAAGGTCTCCAATTTCACTTCATAATGCCAACTTCATCTTCATTTTTGAGACAGTTAAGTGCTAAGGAAACTTGGAAATCAACATCCAGCAGGTGGAGTGGAAAAAACACCTACAACAACACTTCGGGAGTGACTTGTAGTGATTTTGAAACTAATCTGAACCAAATGGAAGGGTTTAACATAAACATGTATGGAAATGAAGAGAGTAGTGGCATGATGGTGAGGAAAAGAGTCATGGTGGTTGTGGATGGATCTTCACATTCTAAACATGCTATGATTTGGGCACTTACTCATGTTGTTAATAAGGGTGATTTGTTCACTTTGCTTCACATTGTTTCTCCTCAAAGGGCTCATTCTGAATCTTATTCTTCTGCTTATCTTGTTAATCATCTTGGGTCACTTTGCAAAGATTGCAAGCCAGAGGTGGAAGTGGAAGCACTTGTAATTCAAGGACCAAAACTTGACACAGTGATGAGCCAGGTCAAGAAATTGGAAGTCTCTATCCTGGTACTAGGCCAAAAGAAGCCACCTTCCCTCTTAAGCTGTCTGTGTGGAAGTAGCAGCTGCAGTAGCACAGAAGAGTTTGTGGAGTACTGTATCAATAATGCAGAGTGCTTGACAATAGGAGTAAGGAAGAGAAGCCAGGGCAATAATGGCTACCTTATCAGCACCAGGTGGCAGAAGAACTTCTGGCTTTTGGCTTAGATTATAGTTTATCATCTGCTAGTAATAGTTTTAGATCTCATTAGAGTGATAAATGGGGAAATAAATTAACCTTCATATTTCTATTGTTATCTACTATAATCTATCTCACTTTGTAACTAATTCTGACTTGTGTATCGAAACTTTACTAATGTAATAAGTCTTGTCATTTTTAGGCATCATGGTAACTGGTACCCAGTTACATGTTTGAAAAYCACCATCTGGTTCATGTTTGTGAACAATACTTTCATACCT
TARGET=128,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03753 Ps_contig_mira-and-tgicl-ass_franssen_30953
SEQUENCE=GCCAYGCTCTAATCAACCTGGTTCATCCTAATTTGRATAGNAAAGCAAAAATGACAAATAAAAGTAATAAAATGGGTGCACATAACAAGCAGGGTATATGTAAATTAGAGTTGCTTGAGGTTGTAAAAAAATTCAGCCCAAATGAATTCTAAATCCCAAATAGATTAATAGGGATGAAATAATAATAATAAAATTAATTAAGACTTATGATGTTCCAGAACAATAACAAAGAAAAATGCTAAAATCCACTCCCTCCGATTTGTAACATCCCTCTGCTTTTTCATTTGAGAACAAACACGGCCCTCAACAATATATATAAAAAATAAAATAAAATCTAATGCTCAGGGATTACCAACCAGAAACGCAATGAAGAAAAGAGTAACGGTGCGGTGAACTCCGACGTGCGTCAACAGCAAGCATCAAACCAATAGATCCAAACATGATTTGAAGATTACCAACGCCTTGTCAACACGGTGTTGCCGGCATCATGAATCCATGGTTACATTTGGTTTCATCATTTAAAACGACGTCATCTGGGTTTCGACATTTCTTCTTCAACCTTCGGATCTTCTCAACGAGTTGATTCTCTTCAAAATCGAGTTGAAGTTTGAGCTTGGCTTGGTTATTTGATTGAGTTGCGTCTGGTGAGTTAAGGTTGATGCGGTAGTGTTTGTTGTCGTTGGAAGCTTCTCACAGCGAATGGTTGTGAGCGGTGCGGTTCGAGTGGATCAAGGTTTATGGAGAGAAAGGTTTATCGAAGGATGTGGTGTGGGTTTCCGCGGCGTGGAAGGCGGAGAGGAAGAAGGAGAAGGTTGGGTTTTGGCGGTGTAGAAAGGGTGTACGTTTGGGATTTCGCAACGACGAGATGAAGAGTAAAGGGGTTGTGCGTTAGTTAAAAGAAGACGCGTGGTGATGGGTGTTGTGAAGAAGAAAAGGAACAATCTATTTTTTCTTTTCTTTATTTTTTTTGTTTATGCTCAACAGAGAGAGACCCCCAAAGGAGAGTATTCTGCGTTGATA
TARGET=178,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03754 Ps_contig_mira-and-tgicl-ass_franssen_30953
SEQUENCE=GCCAYGCTCTAATCAACCTGGTTCATCCTAATTTGRATAGNAAAGCAAAAATGACAAATAAAAGTAATAAAATGGGTGCACATAACAAGCAGGGTATATGTAAATTAGAGTTGCTTGAGGTTGTAAAAAAATTCAGCCCAAATGAATTCTAAATCCCAAATAGATTAATAGGGATGAAATAATAATAATAAAATTAATTAAGACTTATGATGTTCCAGAACAATAACAAAGAAAAATGCTAAAATCCACTCCCTCCGATTTGTAACATCCCTCTGCTTTTTCATTTGAGAACAAACACGGCCCTCAACAATATATATAAAAAATAAAATAAAATCTAATGCTCAGGGATTACCAACCAGAAACGCAATGAAGAAAAGAGTAACGGTGCGGTGAACTCCGACGTGCGTCAACAGCAAGCATCAAACCAATAGATCCAAACATGATTTGAAGATTACCAACGCCTTGTCAACACGGTGTTGCCGGCATCATGAATCCATGGTTACATTTGGTTTCATCATTTAAAACGACGTCATCTGGGTTTCGACATTTCTTCTTCAACCTTCGGATCTTCTCAACGAGTTGATTCTCTTCAAAATCGAGTTGAAGTTTGAGCTTGGCTTGGTTATTTGATTGAGTTGCGTCTGGTGAGTTAAGGTTGATGCGGTAGTGTTTGTTGTCGTTGGAAGCTTCTCACAGCGAATGGTTGTGAGCGGTGCGGTTCGAGTGGATCAAGGTTTATGGAGAGAAAGGTTTATCGAAGGATGTGGTGTGGGTTTCCGCGGCGTGGAAGGCGGAGAGGAAGAAGGAGAAGGTTGGGTTTTGGCGGTGTAGAAAGGGTGTACGTTTGGGATTTCGCAACGACGAGATGAAGAGTAAAGGGGTTGTGCGTTAGTTAAAAGAAGACGCGTGGTGATGGGTGTTGTGAAGAAGAAAAGGAACAATCTATTTTTTCTTTTCTTTATTTTTTTTGTTTATGCTCAACAGAGAGAGACCCCCAAAGGAGAGTATTCTGCGTTGATA
TARGET=320,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03755 Ps_contig_mira-and-tgicl-ass_franssen_30964
SEQUENCE=TCCTTAAAACATGATTATTATAAATATCATGACAATTAAAACAAGAACAAAAACACAGAAACACTTTGTAACTGATATAAAATTGAACACTCAAAACCAACTATAGTAGTATCTACAGCATTATTATAAAGCATCTTGCAGAAAGAAAAAAAACTAAAATTAGTCATGTAACAATAATAGTAATAACTTTGAGGTTATTTGGATGGNNAGTTATTATTATTATTANTAAACAGGACTGTCCTAAGCAGTTCCTGGCCCTGCCATCTCAATAGCCTCAGCACCATCTTACTTACATTCTTCACCACTCATCTCTTCCAATGATTTTCCATTTGCTTCAGGAACTAAGAAGGTGAATACCATTCCAAAGAAGTTAACAACTCCAAGCATAATGAGGGAGTTCTTAATACCAATACCAGCTGGGTAACCTGCATCAGTCTTATTTGGATCCTTATTCTGTGCGGCGTATAAGAATCCGAATGCGCCAACAATTGCTCCAGCTTTTCCTGCAGCGGCCGAGATACCGTGACAGGTAGACCTTAGTCTGGCAGGGAAAATCTCAGCTGGAACAACAAATGTGGTAGCATTTGGACCGAAATTGGCGAAAAAGAAAGTTAATGAGTACATCACGACAAACCCGATTCTGTTTTCTTTCTTTGTCCAGTGATCATAAGGTATGGCTAGAGCAAACATGAAAACAGTCATGAAGAAGAAACCCATTAGTTGGATCGCGAATCGACCCATGTAATCGATGAACGCGACAGTGAACCAGTAACCGGGAACTGTACTGCACATTGCTATAAGTGTTTGTGCTCTTGCAATCCTATAAAGTTCGTGAATTGCATTCATTTCATTCGCCGGAGGAATCCATCCAATGGCAGTGAAAATGTCTTTTGGAAA
TARGET=211,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.8245
PRIMER_LEFT_PENALTY=3.713392
PRIMER_RIGHT_PENALTY=0.111077
PRIMER_LEFT_SEQUENCE=CTTTGAGGTTATTTGGATGGN
PRIMER_RIGHT_SEQUENCE=TCATTGGAAGAGATGAGTGGTG
PRIMER_LEFT=186,21
PRIMER_RIGHT=320,22
PRIMER_LEFT_TM=57.287
PRIMER_RIGHT_TM=60.111
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7750
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=3.8245
PRIMER_LEFT_1_PENALTY=3.713392
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=CTTTGAGGTTATTTGGATGGN
PRIMER_RIGHT_1_SEQUENCE=CATTGGAAGAGATGAGTGGTGA
PRIMER_LEFT_1=186,21
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=57.287
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.7750
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=3.8425
PRIMER_LEFT_2_PENALTY=3.713392
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=CTTTGAGGTTATTTGGATGGN
PRIMER_RIGHT_2_SEQUENCE=GGTTACCCAGCTGGTATTGGTA
PRIMER_LEFT_2=186,21
PRIMER_RIGHT_2=425,22
PRIMER_LEFT_2_TM=57.287
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.7750
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=3.8695
PRIMER_LEFT_3_PENALTY=3.713392
PRIMER_RIGHT_3_PENALTY=0.156061
PRIMER_LEFT_3_SEQUENCE=CTTTGAGGTTATTTGGATGGN
PRIMER_RIGHT_3_SEQUENCE=TGGAAGAGATGAGTGGTGAAGA
PRIMER_LEFT_3=186,21
PRIMER_RIGHT_3=316,22
PRIMER_LEFT_3_TM=57.287
PRIMER_RIGHT_3_TM=59.844
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7750
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=3.9392
PRIMER_LEFT_4_PENALTY=3.713392
PRIMER_RIGHT_4_PENALTY=0.225815
PRIMER_LEFT_4_SEQUENCE=CTTTGAGGTTATTTGGATGGN
PRIMER_RIGHT_4_SEQUENCE=TTCTTATACGCCGCACAGAATA
PRIMER_LEFT_4=186,21
PRIMER_RIGHT_4=470,22
PRIMER_LEFT_4_TM=57.287
PRIMER_RIGHT_4_TM=59.774
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.7750
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_03756 Ps_contig_mira-and-tgicl-ass_franssen_30965
SEQUENCE=AAGGCAGTGGTATCAACGAACAAGAGTACGTCGGGGGTATCTCTTCATCCCAACATCACAACAAATGCTTCTCTCTCCTCAATCCACCAACAAACTGTAACTAACTACCAGTAATCTTTGTAATCAGGTAACAAAGTTGGTGATGGCAGGACAACTAGGAGTGCTTAATGCACTCGATGTGGCAAAGACACAAATGTACCATTTCACAGCAATTGTGATTGCTGGAATGGGTTTTTTCACTGATGCTTATGATCTCTTCTGCATTTCTCTTGTCACAAAGTTGTTAGGAAGAATCTATTACACAGATTACACCCAACCGAAACCGGGTGTGCTTCCTCCCGGTGTTCAAGCCGGTGTAACCGGTGTCGCGCTATGCGGGACTTTAGCCGGCCAACTATTCTTCGGTTGGCTTGGTGACAAATTGGGAAGAAAAAAAGTTTATGGAATGACCCTTATGCTTATGGTAGGTTGTTCACTTGCCTCAGGACTTTCCTTTGGAAGTAGTCCAAAGAGTGTTATGGCAACACTTTGTTTCTTCAGGTTTTGGCTTGGTTTTGGAATTGGTGGTGATTACCCTCTTTCCGCTACAATTATGTCTGAGTATGCTAACAAAAAGCGCGAGGCGCGTTCATCGCTGCGGTGTTTGCTATGCAGGGATTCGGAATCATGGCTGGTGGAATATTTGCCTTGATTGTGGCTACTGCATTTGATCACAAATATAAAGTTCCTACTTATGAAGAAAACCACGAAGCGTCGCTTGTGTTGCCGGCTTTTGACTACGTTTGGCGTTTGATTTTAATGTTTGGTGCTGTTCCAGCAGCTCTTACCTATTACTGGCGTATGAAAATGCCCGAGACGGCTCGTTACACAGCACTTGTTGCTAAGAACGCGAAACAAGCCGCTTCTGATATGTCGAAGGTGTTACAAGTTGAACTTCAAGCTGAAGAGGAGAAAGTGCAGCACATAGTTGAGAACAAAAGCCAAAAGTTTGGCCTATTTAGCAAGCAATTCGCCAAGCGCCACGGTATGCACTTGTTGGGAACAACAACTACTTGGTTCTTGTTAGACATTGCTTTCTACAGTCAGAATCTTTTCCAAAAAGACATTTTCACTGCCATTGGATGGATTCCTCCGGCAAAAGAAATGAATGCGATTCACGAACTTTATAGGATTGCAAGAGCACAGACACTTATAGCAATGTGCAGTACAGTTCCCGGTTACTGGTTCACTGTCGCGTTCATCGATTACATGGGTCGATTCGCAATCCAACTAATGGGTTTCTTCTTCATGACTGTTTTCATGTTTGCTCTAGCCATACCTTATGATCACTGGACAAAGAAAGAAAACAGAATCGGGTTTGTCGTGATGTACTCATTAACTTTCTTTTTCGCCAATTTCGGTCCAAACGCTACCACATTTGTCGTCCCGGCCGAGATTTTCCCCGCCAGACTAAGATCAACCTGTCATGGTATCTCGGCCGCTGCAGGAAAAGCTGGAGCAATTGTTGGCGCATTCGGATTCTTATACGCCGCACAGAATAAGGATCCAAAATTGACTGATGCAGGTTACCCATCTGGTATTGGTATTAAGAACTCTCTCATTATGCTTGGTGTTGTTAACTTCTTTGGAATGGTATTCACCTTCTTAGTTCCTGAAGCAAATGGAAAATCATTGGAAGAGATGAGTGGTGAGAATGAAGAAGATGGTGCTGAGGCTATTGAGATGGCAGGGCCAGGAACTGCTAGGACAGTACCTGTTTAATAATAATAATAATCTCCATCCAAATAACCTCAAAGTTATTACTATTATTGTTACATGACAATTTTTAGTTTTTTTTTCTTCTGCAAGATGCTTTATAATAATGCTGTAGATACTACTATAGTTGGTTTTGAGTGTTCAATTTTATATCAGTTACAAAGTGTTTCTAAGTAA
TARGET=1759,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3611
PRIMER_LEFT_PENALTY=0.111077
PRIMER_RIGHT_PENALTY=2.250018
PRIMER_LEFT_SEQUENCE=TCATTGGAAGAGATGAGTGGTG
PRIMER_RIGHT_SEQUENCE=CTTTGAGGTTATTTGGATGGAG
PRIMER_LEFT=1668,22
PRIMER_RIGHT=1795,22
PRIMER_LEFT_TM=60.111
PRIMER_RIGHT_TM=57.750
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=2.3611
PRIMER_LEFT_1_PENALTY=0.111077
PRIMER_RIGHT_1_PENALTY=2.250018
PRIMER_LEFT_1_SEQUENCE=CATTGGAAGAGATGAGTGGTGA
PRIMER_RIGHT_1_SEQUENCE=CTTTGAGGTTATTTGGATGGAG
PRIMER_LEFT_1=1669,22
PRIMER_RIGHT_1=1795,22
PRIMER_LEFT_1_TM=60.111
PRIMER_RIGHT_1_TM=57.750
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=2.4061
PRIMER_LEFT_2_PENALTY=0.156061
PRIMER_RIGHT_2_PENALTY=2.250018
PRIMER_LEFT_2_SEQUENCE=TGGAAGAGATGAGTGGTGAGAA
PRIMER_RIGHT_2_SEQUENCE=CTTTGAGGTTATTTGGATGGAG
PRIMER_LEFT_2=1672,22
PRIMER_RIGHT_2=1795,22
PRIMER_LEFT_2_TM=59.844
PRIMER_RIGHT_2_TM=57.750
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=2.4061
PRIMER_LEFT_3_PENALTY=0.156061
PRIMER_RIGHT_3_PENALTY=2.250018
PRIMER_LEFT_3_SEQUENCE=TTGGAAGAGATGAGTGGTGAGA
PRIMER_RIGHT_3_SEQUENCE=CTTTGAGGTTATTTGGATGGAG
PRIMER_LEFT_3=1671,22
PRIMER_RIGHT_3=1795,22
PRIMER_LEFT_3_TM=59.844
PRIMER_RIGHT_3_TM=57.750
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=2.4758
PRIMER_LEFT_4_PENALTY=0.225815
PRIMER_RIGHT_4_PENALTY=2.250018
PRIMER_LEFT_4_SEQUENCE=TTCTTATACGCCGCACAGAATA
PRIMER_RIGHT_4_SEQUENCE=CTTTGAGGTTATTTGGATGGAG
PRIMER_LEFT_4=1518,22
PRIMER_RIGHT_4=1795,22
PRIMER_LEFT_4_TM=59.774
PRIMER_RIGHT_4_TM=57.750
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_03757 Ps_contig_mira-and-tgicl-ass_franssen_30966
SEQUENCE=ACTACTACTATTTCTCTTTTGTACTTATTACTCTCTCGCTCTCTCCTCTAACAAAACATTAACGTCTTCCTTCTTCCTCTTTCTCTCTACTCACACTAACAAACAACCAAACAATATGTTTCTCTCTCTCTTATTTCTCATCTTCTTCCTCCTTCAATCTCCTCTCCCATCCCTCTCAACAAACTCTGAAGGAAACGCTTTACATGCTTTGAGAACCAGACTTTATGATCCCAACAACGTTCTTCAAAGCTGGGACCCAACTCTTGTTAACTCCTGTACTTGGTTCCATGTTACCTGTGACTCCAACAACCACGTTATTCGCCTAGACTTGGGTAATTCTAACATCTCTGGTTCTTTGGGACCTGAACTCGCCCAATTGACTCACCTTCAATACTTGGAGTTATATAATAATAATATTAATGGCAACATTCCTCAGGAACTTGGTAATTTAAAGAACCTTATTAGTATGGATTTGTATGATAATCAATTCGAAGGTAATATCCCAAAGTCCTTTAGCAACTTGAAGACACTTAAATTCCTGAGGCTAAACAACAATAAGCTCACAGGACCTATCCCAAGAGAACTTACTCATCTTAAAAATCTCAAAATTCTTGATGTTTCCAATAATGACCTCTGTGGTACCATACCAGTAGATGGAAACTTTGGATCTTTCCCAGCAGAAAGTTTTGAAAATAACGCGCTCAACGGCCCAGAACTGAAAGGACTGGTTCCTTATGACTTTGGATGCTAAGAAAGGTTGAGGAATACTGGCCTCCTCAGATTGTTATGAAGAGGCTTCTTGACATGGGCAGTTAGTAGTATCAGAGATGTATGATTGCTACAAAATGAATCTAAGAGACAGGTGTATGTCAAAATATATGTAATAGTAAATAAGATGATATTATCATCTGTCTCTCTGGTTAGGCTGCATAGCAAGACTAGGATCATGTTGATTATCAAGTAAACGTGTTTCTAGTAATTTTTTCGCTTTTATATACATTGTGGTTTGATGCCTAATTCTTCTGTTTAATTTGGCCGTCCATTGGAAGTTTGGCTGAATTTATGTTCCAGCACAGGGCGAGTTATTCAGACGCAGTATTCAATTATGACAAATCCTGTACCTTAATTTAATGCAGTGTAAACTATGTTCCTGCAAAGCATTCTTCTGAGTTTACGTTATTGTAGTTTCAATGTACTCATTTAAAAAAAANTAGTAGTTGTGTAGTTTTATCTGGNCT
TARGET=121,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0814
PRIMER_LEFT_PENALTY=1.008825
PRIMER_RIGHT_PENALTY=0.072552
PRIMER_LEFT_SEQUENCE=CGTCTTCCTTCTTCCTCTTTCTC
PRIMER_RIGHT_SEQUENCE=AAAGCGTTTCCTTCAGAGTTTG
PRIMER_LEFT=62,23
PRIMER_RIGHT=200,22
PRIMER_LEFT_TM=60.009
PRIMER_RIGHT_TM=59.927
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=139
PRIMER_PAIR_PENALTY_1=1.1581
PRIMER_LEFT_1_PENALTY=1.085505
PRIMER_RIGHT_1_PENALTY=0.072552
PRIMER_LEFT_1_SEQUENCE=TCTCTCGCTCTCTCCTCTAACAA
PRIMER_RIGHT_1_SEQUENCE=AAAGCGTTTCCTTCAGAGTTTG
PRIMER_LEFT_1=31,23
PRIMER_RIGHT_1=200,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=59.927
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=1.2516
PRIMER_LEFT_2_PENALTY=1.008825
PRIMER_RIGHT_2_PENALTY=0.242742
PRIMER_LEFT_2_SEQUENCE=CGTCTTCCTTCTTCCTCTTTCTC
PRIMER_RIGHT_2_SEQUENCE=GTCACAGGTAACATGGAACCAA
PRIMER_LEFT_2=62,23
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=60.009
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=1.2787
PRIMER_LEFT_3_PENALTY=1.208920
PRIMER_RIGHT_3_PENALTY=0.069737
PRIMER_LEFT_3_SEQUENCE=TCTCGCTCTCTCCTCTAACAAAA
PRIMER_RIGHT_3_SEQUENCE=GTGGTTGTTGGAGTCACAGGTA
PRIMER_LEFT_3=33,23
PRIMER_RIGHT_3=312,22
PRIMER_LEFT_3_TM=59.791
PRIMER_RIGHT_3_TM=59.930
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=1.2803
PRIMER_LEFT_4_PENALTY=1.210595
PRIMER_RIGHT_4_PENALTY=0.069737
PRIMER_LEFT_4_SEQUENCE=CGCTCTCTCCTCTAACAAAACA
PRIMER_RIGHT_4_SEQUENCE=GTGGTTGTTGGAGTCACAGGTA
PRIMER_LEFT_4=36,22
PRIMER_RIGHT_4=312,22
PRIMER_LEFT_4_TM=58.789
PRIMER_RIGHT_4_TM=59.930
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=277
=
PRIMER_SEQUENCE_ID=SSR_03758 Ps_contig_mira-and-tgicl-ass_franssen_30966
SEQUENCE=ACTACTACTATTTCTCTTTTGTACTTATTACTCTCTCGCTCTCTCCTCTAACAAAACATTAACGTCTTCCTTCTTCCTCTTTCTCTCTACTCACACTAACAAACAACCAAACAATATGTTTCTCTCTCTCTTATTTCTCATCTTCTTCCTCCTTCAATCTCCTCTCCCATCCCTCTCAACAAACTCTGAAGGAAACGCTTTACATGCTTTGAGAACCAGACTTTATGATCCCAACAACGTTCTTCAAAGCTGGGACCCAACTCTTGTTAACTCCTGTACTTGGTTCCATGTTACCTGTGACTCCAACAACCACGTTATTCGCCTAGACTTGGGTAATTCTAACATCTCTGGTTCTTTGGGACCTGAACTCGCCCAATTGACTCACCTTCAATACTTGGAGTTATATAATAATAATATTAATGGCAACATTCCTCAGGAACTTGGTAATTTAAAGAACCTTATTAGTATGGATTTGTATGATAATCAATTCGAAGGTAATATCCCAAAGTCCTTTAGCAACTTGAAGACACTTAAATTCCTGAGGCTAAACAACAATAAGCTCACAGGACCTATCCCAAGAGAACTTACTCATCTTAAAAATCTCAAAATTCTTGATGTTTCCAATAATGACCTCTGTGGTACCATACCAGTAGATGGAAACTTTGGATCTTTCCCAGCAGAAAGTTTTGAAAATAACGCGCTCAACGGCCCAGAACTGAAAGGACTGGTTCCTTATGACTTTGGATGCTAAGAAAGGTTGAGGAATACTGGCCTCCTCAGATTGTTATGAAGAGGCTTCTTGACATGGGCAGTTAGTAGTATCAGAGATGTATGATTGCTACAAAATGAATCTAAGAGACAGGTGTATGTCAAAATATATGTAATAGTAAATAAGATGATATTATCATCTGTCTCTCTGGTTAGGCTGCATAGCAAGACTAGGATCATGTTGATTATCAAGTAAACGTGTTTCTAGTAATTTTTTCGCTTTTATATACATTGTGGTTTGATGCCTAATTCTTCTGTTTAATTTGGCCGTCCATTGGAAGTTTGGCTGAATTTATGTTCCAGCACAGGGCGAGTTATTCAGACGCAGTATTCAATTATGACAAATCCTGTACCTTAATTTAATGCAGTGTAAACTATGTTCCTGCAAAGCATTCTTCTGAGTTTACGTTATTGTAGTTTCAATGTACTCATTTAAAAAAAANTAGTAGTTGTGTAGTTTTATCTGGNCT
TARGET=405,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1941
PRIMER_LEFT_PENALTY=0.069737
PRIMER_RIGHT_PENALTY=0.124370
PRIMER_LEFT_SEQUENCE=TACCTGTGACTCCAACAACCAC
PRIMER_RIGHT_SEQUENCE=CTCTTGGGATAGGTCCTGTGAG
PRIMER_LEFT=291,22
PRIMER_RIGHT=580,22
PRIMER_LEFT_TM=59.930
PRIMER_RIGHT_TM=60.124
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=290
PRIMER_PAIR_PENALTY_1=0.4942
PRIMER_LEFT_1_PENALTY=0.369874
PRIMER_RIGHT_1_PENALTY=0.124370
PRIMER_LEFT_1_SEQUENCE=TTCGCCTAGACTTGGGTAATTC
PRIMER_RIGHT_1_SEQUENCE=CTCTTGGGATAGGTCCTGTGAG
PRIMER_LEFT_1=317,22
PRIMER_RIGHT_1=580,22
PRIMER_LEFT_1_TM=59.630
PRIMER_RIGHT_1_TM=60.124
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.5343
PRIMER_LEFT_2_PENALTY=0.409918
PRIMER_RIGHT_2_PENALTY=0.124370
PRIMER_LEFT_2_SEQUENCE=TCCATGTTACCTGTGACTCCAA
PRIMER_RIGHT_2_SEQUENCE=CTCTTGGGATAGGTCCTGTGAG
PRIMER_LEFT_2=284,22
PRIMER_RIGHT_2=580,22
PRIMER_LEFT_2_TM=60.410
PRIMER_RIGHT_2_TM=60.124
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=0.5343
PRIMER_LEFT_3_PENALTY=0.409918
PRIMER_RIGHT_3_PENALTY=0.124370
PRIMER_LEFT_3_SEQUENCE=TTCCATGTTACCTGTGACTCCA
PRIMER_RIGHT_3_SEQUENCE=CTCTTGGGATAGGTCCTGTGAG
PRIMER_LEFT_3=283,22
PRIMER_RIGHT_3=580,22
PRIMER_LEFT_3_TM=60.410
PRIMER_RIGHT_3_TM=60.124
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.5573
PRIMER_LEFT_4_PENALTY=0.069737
PRIMER_RIGHT_4_PENALTY=0.487583
PRIMER_LEFT_4_SEQUENCE=TACCTGTGACTCCAACAACCAC
PRIMER_RIGHT_4_SEQUENCE=TTCTCTTGGGATAGGTCCTGTG
PRIMER_LEFT_4=291,22
PRIMER_RIGHT_4=582,22
PRIMER_LEFT_4_TM=59.930
PRIMER_RIGHT_4_TM=60.488
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_03759 Ps_contig_mira-and-tgicl-ass_franssen_30968
SEQUENCE=AAAGTCAACAAAGCTAACTTTYATACCTTAAAGCAATATAAATAAATAAAAAGTATTAGATCATAGAATCATACCCATTACATCCCTCACTTTTATTATTTATTTCCACCTTCTCTCATAAAAAGTGAGGAATATTTAATTTGCATGACATTTTAACTTAGGAAAGTCAAGTCACACTACTATTGCATCATATACAAATGACAACATAGAATACCAAATAAAATACGTTCAGAGTGCTCTTATTAGAGTCCAATGAGTAACGTGACCAACACAATTCGTCGTAGCATAGATTCCATTACATAAAAGCACTACTAGCCTAGTAATATTTCACACAATACTAGCTGCTACTAAATTATCTTCACTTAGTGGAGCTGAATTTTACATAGGCTGAGAATAAGCACCTAATATCCTTTTCATCATGAAGAAGAGTGCTGACCCTCGCAAGCAGGGCTATCATCAAAATTCTTCGAAGCATACTTCGCGGTGACCACACCAACCTCAAAAACAATATCGTCACCGGAAGAGCGAATTCCGGTGATAGACCACCACTTAAAGAAAGCACGAACCCTAATTCCGTCAAGCTCGGCGATCTTACCTTTCACAATTTTCCCGGTGATAGTGTCGGAATAGGTAGCAACATAGTTATCGGGAGGAAGCGTGATTTTACACGCGCCACCGAGCTTGACGGAGAAATCGCCGGATGTTTGGTTAATGACGTGGGATAAGACGGTGGTGTCGGGAAGAAGACCAACGGGGAAACCATAACTGGCGAGCTCAAGGTGTGCCGGTGATGGAGTCGATTTGGGGATTTTAGGATCAGGGTTAGGGTTTTGGAACGATTTTGATATGAGATTCAGAAAAAGAACGAACACAATGAGAATTTTGGGATCCATGGTTTTTCTTTTTTCGTTTGAATTCTTATTTTTCACTATAGACTCTCCTCCCCGAC
TARGET=38,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03760 Ps_contig_mira-and-tgicl-ass_franssen_30972
SEQUENCE=CCACAATCACACAACTAGTTTAAAACTGAGTTTCAAAATATTTGTATAAAAAAAGAATGAGAAATTTATAATCTCTAATCATTGTACATACAACAGTATTTGACATGGAAGTCTAAAGGACATTAATGTACTAGCCACTGGTTGAGGGAATGTATAGTCAATTTTCTCTAGAGGAACTTGTAACACCAGAATTCAACAGGGATCAATCAATCAATCAACCCTTTTTGCCAAGAAAATTCCTAAGCTTGCTTCTTGAAATTCCAATCAATATCTCCACTAGCTGCTGTTTTAGCAATTTCTTCGGCCTTTTCATCGTTGAAACCCAACGCAGACAAGACCTTTCGACCTGCCGATTCTTCTTGTGACCAAATTCCAATAAGTAAATGAGTCACGTTTGTTTCTCCTTCTTCACCTGACTTCAATTTCTCGTTAATTGCCCAATCGAGGGCTTTCTGAAGTGGTTTGGTTAAAGAAGGAATTATTGGGCTGGAGAAGTGAAAGTCAGATTTCCCAAGTAGCTCTAATGTTTGTTTGCGTACTTTCAAATACGTAATTCCGTTAGCTCTTAAGAATTTTGCAGCTTTACTTGTTCCTTCAACCAAAATCCCCATGAGAAGAGTCTCAGTCCCTACTTTAGGATACTTCAGCTTCCTTGTTTCCAACTCTGCCATTGCATATGATTTTATAGCCCTCGCAGACCACTTTGGATTTGGGGTTTTCTTCGGTTCACCGGATTGTGGATTCGGGTCGGTGGGGGGACTAAACGGCACCGTTGCCGAGGAAGCACGGCAGTTGGTGATGGAAATTGAACGAGTTGAGGAAATGGGTCGTTGAAGGACGAGCTTTGTTGGAAAGAGAGAAGTGAAAGAGAGAGTGCATGATTGGTTGTTGTTGAGGTGTGAATGTGATAAGTTTGAATGGGGAAAGAGTGTGTTGGTGTTGGATTTGAGTAGAAGAGAAGAAGAAGAGAAAGTTGGAATGGAAGCCATTGGAGAGAGAGAACTGTTGGGTGGGTTTGGATGAATTTGTTATGTGCACAACCCTCT
TARGET=202,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6731
PRIMER_LEFT_PENALTY=0.529160
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=TAACACCAGAATTCAACAGGGA
PRIMER_RIGHT_SEQUENCE=GGTGAAGAAGGAGAAACAAACG
PRIMER_LEFT=180,22
PRIMER_RIGHT=412,22
PRIMER_LEFT_TM=59.471
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.6924
PRIMER_LEFT_1_PENALTY=0.548390
PRIMER_RIGHT_1_PENALTY=0.143968
PRIMER_LEFT_1_SEQUENCE=GTACTAGCCACTGGTTGAGGGA
PRIMER_RIGHT_1_SEQUENCE=GGTGAAGAAGGAGAAACAAACG
PRIMER_LEFT_1=127,22
PRIMER_RIGHT_1=412,22
PRIMER_LEFT_1_TM=60.548
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.7361
PRIMER_LEFT_2_PENALTY=0.529160
PRIMER_RIGHT_2_PENALTY=0.206979
PRIMER_LEFT_2_SEQUENCE=TAACACCAGAATTCAACAGGGA
PRIMER_RIGHT_2_SEQUENCE=TGCTAAAACAGCAGCTAGTGGA
PRIMER_LEFT_2=180,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=59.471
PRIMER_RIGHT_2_TM=60.207
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=0.7805
PRIMER_LEFT_3_PENALTY=0.529160
PRIMER_RIGHT_3_PENALTY=0.251350
PRIMER_LEFT_3_SEQUENCE=TAACACCAGAATTCAACAGGGA
PRIMER_RIGHT_3_SEQUENCE=GCCGAAGAAATTGCTAAAACAG
PRIMER_LEFT_3=180,22
PRIMER_RIGHT_3=304,22
PRIMER_LEFT_3_TM=59.471
PRIMER_RIGHT_3_TM=60.251
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=0.7805
PRIMER_LEFT_4_PENALTY=0.529160
PRIMER_RIGHT_4_PENALTY=0.251350
PRIMER_LEFT_4_SEQUENCE=TAACACCAGAATTCAACAGGGA
PRIMER_RIGHT_4_SEQUENCE=CCGAAGAAATTGCTAAAACAGC
PRIMER_LEFT_4=180,22
PRIMER_RIGHT_4=303,22
PRIMER_LEFT_4_TM=59.471
PRIMER_RIGHT_4_TM=60.251
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_03761 Ps_contig_mira-and-tgicl-ass_franssen_30972
SEQUENCE=CCACAATCACACAACTAGTTTAAAACTGAGTTTCAAAATATTTGTATAAAAAAAGAATGAGAAATTTATAATCTCTAATCATTGTACATACAACAGTATTTGACATGGAAGTCTAAAGGACATTAATGTACTAGCCACTGGTTGAGGGAATGTATAGTCAATTTTCTCTAGAGGAACTTGTAACACCAGAATTCAACAGGGATCAATCAATCAATCAACCCTTTTTGCCAAGAAAATTCCTAAGCTTGCTTCTTGAAATTCCAATCAATATCTCCACTAGCTGCTGTTTTAGCAATTTCTTCGGCCTTTTCATCGTTGAAACCCAACGCAGACAAGACCTTTCGACCTGCCGATTCTTCTTGTGACCAAATTCCAATAAGTAAATGAGTCACGTTTGTTTCTCCTTCTTCACCTGACTTCAATTTCTCGTTAATTGCCCAATCGAGGGCTTTCTGAAGTGGTTTGGTTAAAGAAGGAATTATTGGGCTGGAGAAGTGAAAGTCAGATTTCCCAAGTAGCTCTAATGTTTGTTTGCGTACTTTCAAATACGTAATTCCGTTAGCTCTTAAGAATTTTGCAGCTTTACTTGTTCCTTCAACCAAAATCCCCATGAGAAGAGTCTCAGTCCCTACTTTAGGATACTTCAGCTTCCTTGTTTCCAACTCTGCCATTGCATATGATTTTATAGCCCTCGCAGACCACTTTGGATTTGGGGTTTTCTTCGGTTCACCGGATTGTGGATTCGGGTCGGTGGGGGGACTAAACGGCACCGTTGCCGAGGAAGCACGGCAGTTGGTGATGGAAATTGAACGAGTTGAGGAAATGGGTCGTTGAAGGACGAGCTTTGTTGGAAAGAGAGAAGTGAAAGAGAGAGTGCATGATTGGTTGTTGTTGAGGTGTGAATGTGATAAGTTTGAATGGGGAAAGAGTGTGTTGGTGTTGGATTTGAGTAGAAGAGAAGAAGAAGAGAAAGTTGGAATGGAAGCCATTGGAGAGAGAGAACTGTTGGGTGGGTTTGGATGAATTTGTTATGTGCACAACCCTCT
TARGET=957,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0649
PRIMER_LEFT_PENALTY=0.048588
PRIMER_RIGHT_PENALTY=0.016312
PRIMER_LEFT_SEQUENCE=TGTTGTTGAGGTGTGAATGTGA
PRIMER_RIGHT_SEQUENCE=CAGTTCTCTCTCTCCAATGGCT
PRIMER_LEFT=886,22
PRIMER_RIGHT=1004,22
PRIMER_LEFT_TM=60.049
PRIMER_RIGHT_TM=60.016
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.1118
PRIMER_LEFT_1_PENALTY=0.095523
PRIMER_RIGHT_1_PENALTY=0.016312
PRIMER_LEFT_1_SEQUENCE=GAGTGCATGATTGGTTGTTGTT
PRIMER_RIGHT_1_SEQUENCE=CAGTTCTCTCTCTCCAATGGCT
PRIMER_LEFT_1=871,22
PRIMER_RIGHT_1=1004,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=60.016
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=0.1638
PRIMER_LEFT_2_PENALTY=0.048588
PRIMER_RIGHT_2_PENALTY=0.115189
PRIMER_LEFT_2_SEQUENCE=TGTTGTTGAGGTGTGAATGTGA
PRIMER_RIGHT_2_SEQUENCE=ACAGTTCTCTCTCTCCAATGGC
PRIMER_LEFT_2=886,22
PRIMER_RIGHT_2=1005,22
PRIMER_LEFT_2_TM=60.049
PRIMER_RIGHT_2_TM=59.885
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=0.2107
PRIMER_LEFT_3_PENALTY=0.095523
PRIMER_RIGHT_3_PENALTY=0.115189
PRIMER_LEFT_3_SEQUENCE=GAGTGCATGATTGGTTGTTGTT
PRIMER_RIGHT_3_SEQUENCE=ACAGTTCTCTCTCTCCAATGGC
PRIMER_LEFT_3=871,22
PRIMER_RIGHT_3=1005,22
PRIMER_LEFT_3_TM=59.904
PRIMER_RIGHT_3_TM=59.885
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=0.2591
PRIMER_LEFT_4_PENALTY=0.242742
PRIMER_RIGHT_4_PENALTY=0.016312
PRIMER_LEFT_4_SEQUENCE=ATGATTGGTTGTTGTTGAGGTG
PRIMER_RIGHT_4_SEQUENCE=CAGTTCTCTCTCTCCAATGGCT
PRIMER_LEFT_4=877,22
PRIMER_RIGHT_4=1004,22
PRIMER_LEFT_4_TM=59.757
PRIMER_RIGHT_4_TM=60.016
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_03762 Ps_contig_mira-and-tgicl-ass_franssen_30972
SEQUENCE=CCACAATCACACAACTAGTTTAAAACTGAGTTTCAAAATATTTGTATAAAAAAAGAATGAGAAATTTATAATCTCTAATCATTGTACATACAACAGTATTTGACATGGAAGTCTAAAGGACATTAATGTACTAGCCACTGGTTGAGGGAATGTATAGTCAATTTTCTCTAGAGGAACTTGTAACACCAGAATTCAACAGGGATCAATCAATCAATCAACCCTTTTTGCCAAGAAAATTCCTAAGCTTGCTTCTTGAAATTCCAATCAATATCTCCACTAGCTGCTGTTTTAGCAATTTCTTCGGCCTTTTCATCGTTGAAACCCAACGCAGACAAGACCTTTCGACCTGCCGATTCTTCTTGTGACCAAATTCCAATAAGTAAATGAGTCACGTTTGTTTCTCCTTCTTCACCTGACTTCAATTTCTCGTTAATTGCCCAATCGAGGGCTTTCTGAAGTGGTTTGGTTAAAGAAGGAATTATTGGGCTGGAGAAGTGAAAGTCAGATTTCCCAAGTAGCTCTAATGTTTGTTTGCGTACTTTCAAATACGTAATTCCGTTAGCTCTTAAGAATTTTGCAGCTTTACTTGTTCCTTCAACCAAAATCCCCATGAGAAGAGTCTCAGTCCCTACTTTAGGATACTTCAGCTTCCTTGTTTCCAACTCTGCCATTGCATATGATTTTATAGCCCTCGCAGACCACTTTGGATTTGGGGTTTTCTTCGGTTCACCGGATTGTGGATTCGGGTCGGTGGGGGGACTAAACGGCACCGTTGCCGAGGAAGCACGGCAGTTGGTGATGGAAATTGAACGAGTTGAGGAAATGGGTCGTTGAAGGACGAGCTTTGTTGGAAAGAGAGAAGTGAAAGAGAGAGTGCATGATTGGTTGTTGTTGAGGTGTGAATGTGATAAGTTTGAATGGGGAAAGAGTGTGTTGGTGTTGGATTTGAGTAGAAGAGAAGAAGAAGAGAAAGTTGGAATGGAAGCCATTGGAGAGAGAGAACTGTTGGGTGGGTTTGGATGAATTTGTTATGTGCACAACCCTCT
TARGET=992,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5091
PRIMER_LEFT_PENALTY=0.048588
PRIMER_RIGHT_PENALTY=0.460467
PRIMER_LEFT_SEQUENCE=TGTTGTTGAGGTGTGAATGTGA
PRIMER_RIGHT_SEQUENCE=AGAGGGTTGTGCACATAACAAA
PRIMER_LEFT=886,22
PRIMER_RIGHT=1045,22
PRIMER_LEFT_TM=60.049
PRIMER_RIGHT_TM=59.540
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=0.5112
PRIMER_LEFT_1_PENALTY=0.050763
PRIMER_RIGHT_1_PENALTY=0.460467
PRIMER_LEFT_1_SEQUENCE=GAAAGAGTGTGTTGGTGTTGGA
PRIMER_RIGHT_1_SEQUENCE=AGAGGGTTGTGCACATAACAAA
PRIMER_LEFT_1=922,22
PRIMER_RIGHT_1=1045,22
PRIMER_LEFT_1_TM=60.051
PRIMER_RIGHT_1_TM=59.540
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.5145
PRIMER_LEFT_2_PENALTY=0.054021
PRIMER_RIGHT_2_PENALTY=0.460467
PRIMER_LEFT_2_SEQUENCE=ACGAGCTTTGTTGGAAAGAGAG
PRIMER_RIGHT_2_SEQUENCE=AGAGGGTTGTGCACATAACAAA
PRIMER_LEFT_2=837,22
PRIMER_RIGHT_2=1045,22
PRIMER_LEFT_2_TM=60.054
PRIMER_RIGHT_2_TM=59.540
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.7730
PRIMER_LEFT_3_PENALTY=0.312496
PRIMER_RIGHT_3_PENALTY=0.460467
PRIMER_LEFT_3_SEQUENCE=TGTGTTGGTGTTGGATTTGAGT
PRIMER_RIGHT_3_SEQUENCE=AGAGGGTTGTGCACATAACAAA
PRIMER_LEFT_3=929,22
PRIMER_RIGHT_3=1045,22
PRIMER_LEFT_3_TM=60.312
PRIMER_RIGHT_3_TM=59.540
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=117
PRIMER_PAIR_PENALTY_4=0.7730
PRIMER_LEFT_4_PENALTY=0.312496
PRIMER_RIGHT_4_PENALTY=0.460467
PRIMER_LEFT_4_SEQUENCE=GTGTGTTGGTGTTGGATTTGAG
PRIMER_RIGHT_4_SEQUENCE=AGAGGGTTGTGCACATAACAAA
PRIMER_LEFT_4=928,22
PRIMER_RIGHT_4=1045,22
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=59.540
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_03763 Ps_contig_mira-and-tgicl-ass_franssen_30974
SEQUENCE=GGTTTCAAGGAACGCACCAAATTCAATTGCAAGCATRGNRTGGTGCAATAAAAATACAAAGGAATAAAATGCATTAATACAATAGGACTCGTTTGATAATACAAGGAGTACAGTGTGATAAGCAACCTCGGATCCTAATCATAAACCACAACACTGATTACACACATATATATAAATAATACTTTCTCTTCTTTGCTTTCACCCATTGCAACTCACTTTTACAGCTCCTATCTTTCAGAAAATACTGTGGAGCTAGTTACACATAAATATTGGTCTTCTGTTTCACTAGCCGCCGCATTGTTATGATTCTGCAGGAGGGTTTTGAATGCAGCAAAACCATTTTTTACTCTGAGGTTTGCCGGGATCTGATGCAGGTTTGCTTTGAGGTTCAGTAGTAGTCTTTCCTGGTGACTCGGTATTGCCACCTGTCTTTTTCTCATCCCTTGCCTTATTAAAAATCACTGTGAACCCCTCAGCTGAGGCTGGATCGTTGACATCCCATTCACCGAATTTGGGTAGTGGAGGACCCTTTTCCGACATCAATGCAAGAGAGATGAAATATTTTGTAGGAACAGAAAAGGAGTGAGAAAACGCGAATAAATAAAAAGGAATCGGAAAAAGAAGAAAAAACTGAGGAAGAGAAAGTTAGAGAGAGAGAGAGAGAGAATGCGTTCCCNCGTAGAAGACCTTGGAGCGACTAGTAACGNGTGTGAAACTCCCCCGTATCTGCGTTGACTACCACTGCTT
TARGET=648,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03764 Ps_contig_mira-and-tgicl-ass_franssen_30978
SEQUENCE=CGGGGTATACACAAATACAATGTTTAGATAGATAGAATAGAATAATAGAATAGAATAGAATATAGTTGTACTATATTATTTTATTTTTTCTGGTGTAAATCACTGGTTTGGCCAAAGAACACGAGCCAAAGATTACGTGGCGGCTAAGATTTGTTTCTGACTTTTTGTTGCAAACTAGATAGATAGAGAAAAACAACACCACAACAATGGCTACTTTGAATGTTTTCTCTCAAACATTGCACCCTACTATTTCATCTTCTTTCTGTTCTTCTTCTTCTTCTTATTCAAATTCTAACTATGGTTCTTTCTGTCCAAATTCTAACACTTCTAAGTTTCATTTCTTTTCCAATTCTTCTTCCCTTTCTGCCCCTACCCTTTCTAACTCTTCTAAGACCCTTTTCACAAAGTCAGGTAGTGGAGGTTCATGGCTGCAAAGTAGTTCGGCATCTGTCAGCAACGAGCAAAAGAACGGTCCTGTCTATTCAGTATTTCCGTCATCGCCTGCTCAAGTTTCCTCTGTGCAAGACCTGTACAAATTTATTTGCTCAGGACCTTTGCTTGACAGAATTGGTTTGACGCCAGAGAACGTGGCCGAGTCTATAGATAAGTGGTTAACCTACGGGCGACAACTATGCAGACTGTTTCAGCTTAACGAATTGTTCTTGACCGAGCCTCAAAAAGTTAGGCTTTATCATTACTATATACCGGTCTTCCTCTGGTGCGAAAGTGAGATTGTTCAGCATCGGTCCAAATTCAAAGACGGAGAAGATATACCTCCTTTAGTGATTGGATTTAGTGCTCCTCAAGGCTGTGGAAAGACGACCCTCGTCTTTGCTCTCGACTATCTTTTCCAAATGATTGGCAGGAAGTCTGCAACAATATCGATAGATGATTTTTATTTGACGGCTGAAGGTCAGAATAAACTAAGAGAAGCTAATCCAGGAAATGCACTTCTTGAGTTTCGTGGAAATGCAGGAAGCCATGATCTTCCTATATCTGTTGAAACTCTGTCGGCTTTAACCAAAATGTCGAAAGAAGGTATCAAGATGAAGTTACCAAGATATGATAAATCTGCTTTCGGTGGGAGAGGTGACCGCGCTGATCCTTCAACATGGCCTGAAGTTGAAGGGCCACTTGAAGTTGTGTTATTTGAAGGCTGGATGCTTGGTTTCAAGCCCGTTCCAGTTGATGCTGTAAAATCGGTTGATCCTCAGCTGGAAATAATAAATAAGAACCTCGAAGCTTATTATGATGCGTGGGACAAATATATAAAGTCATGGATAGTCATTAAGATTAAGGATCCAAGTTGTGTCTACCAGTGGCGCTTGCAGGCTGAGATTGCGATGAGGGAGGCAGGAAATCCTGGAATGTCTGATGATGAGGTGAATGATTTTGTTTCGCGCTACCTGCCAGCATACAACGCGTACCTTCCTACACTTTACTCGCAGGGTCCGAATGGATCAGATCCTCAACATCTACTAACCATTGAAATTGATGAAAAGAGAAACCCGATCCTAGGTGTCTAGTTTAACTAGATGACGATAGGACAATGTTGGGTTTTTTCCATTCTTTGAGAGAATCGAGATGATCGTTCGGCACTCGTGGAGGAGACAAGTCAATGTATCAGCACTATTTTCTTTGAAATGTATAGAGGATATTCTTATCAGAACATAATAATAATAATAATAATGCCTATTCCATTAACTTTT
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03765 Ps_contig_mira-and-tgicl-ass_franssen_30978
SEQUENCE=CGGGGTATACACAAATACAATGTTTAGATAGATAGAATAGAATAATAGAATAGAATAGAATATAGTTGTACTATATTATTTTATTTTTTCTGGTGTAAATCACTGGTTTGGCCAAAGAACACGAGCCAAAGATTACGTGGCGGCTAAGATTTGTTTCTGACTTTTTGTTGCAAACTAGATAGATAGAGAAAAACAACACCACAACAATGGCTACTTTGAATGTTTTCTCTCAAACATTGCACCCTACTATTTCATCTTCTTTCTGTTCTTCTTCTTCTTCTTATTCAAATTCTAACTATGGTTCTTTCTGTCCAAATTCTAACACTTCTAAGTTTCATTTCTTTTCCAATTCTTCTTCCCTTTCTGCCCCTACCCTTTCTAACTCTTCTAAGACCCTTTTCACAAAGTCAGGTAGTGGAGGTTCATGGCTGCAAAGTAGTTCGGCATCTGTCAGCAACGAGCAAAAGAACGGTCCTGTCTATTCAGTATTTCCGTCATCGCCTGCTCAAGTTTCCTCTGTGCAAGACCTGTACAAATTTATTTGCTCAGGACCTTTGCTTGACAGAATTGGTTTGACGCCAGAGAACGTGGCCGAGTCTATAGATAAGTGGTTAACCTACGGGCGACAACTATGCAGACTGTTTCAGCTTAACGAATTGTTCTTGACCGAGCCTCAAAAAGTTAGGCTTTATCATTACTATATACCGGTCTTCCTCTGGTGCGAAAGTGAGATTGTTCAGCATCGGTCCAAATTCAAAGACGGAGAAGATATACCTCCTTTAGTGATTGGATTTAGTGCTCCTCAAGGCTGTGGAAAGACGACCCTCGTCTTTGCTCTCGACTATCTTTTCCAAATGATTGGCAGGAAGTCTGCAACAATATCGATAGATGATTTTTATTTGACGGCTGAAGGTCAGAATAAACTAAGAGAAGCTAATCCAGGAAATGCACTTCTTGAGTTTCGTGGAAATGCAGGAAGCCATGATCTTCCTATATCTGTTGAAACTCTGTCGGCTTTAACCAAAATGTCGAAAGAAGGTATCAAGATGAAGTTACCAAGATATGATAAATCTGCTTTCGGTGGGAGAGGTGACCGCGCTGATCCTTCAACATGGCCTGAAGTTGAAGGGCCACTTGAAGTTGTGTTATTTGAAGGCTGGATGCTTGGTTTCAAGCCCGTTCCAGTTGATGCTGTAAAATCGGTTGATCCTCAGCTGGAAATAATAAATAAGAACCTCGAAGCTTATTATGATGCGTGGGACAAATATATAAAGTCATGGATAGTCATTAAGATTAAGGATCCAAGTTGTGTCTACCAGTGGCGCTTGCAGGCTGAGATTGCGATGAGGGAGGCAGGAAATCCTGGAATGTCTGATGATGAGGTGAATGATTTTGTTTCGCGCTACCTGCCAGCATACAACGCGTACCTTCCTACACTTTACTCGCAGGGTCCGAATGGATCAGATCCTCAACATCTACTAACCATTGAAATTGATGAAAAGAGAAACCCGATCCTAGGTGTCTAGTTTAACTAGATGACGATAGGACAATGTTGGGTTTTTTCCATTCTTTGAGAGAATCGAGATGATCGTTCGGCACTCGTGGAGGAGACAAGTCAATGTATCAGCACTATTTTCTTTGAAATGTATAGAGGATATTCTTATCAGAACATAATAATAATAATAATAATGCCTATTCCATTAACTTTT
TARGET=44,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03766 Ps_contig_mira-and-tgicl-ass_franssen_30978
SEQUENCE=CGGGGTATACACAAATACAATGTTTAGATAGATAGAATAGAATAATAGAATAGAATAGAATATAGTTGTACTATATTATTTTATTTTTTCTGGTGTAAATCACTGGTTTGGCCAAAGAACACGAGCCAAAGATTACGTGGCGGCTAAGATTTGTTTCTGACTTTTTGTTGCAAACTAGATAGATAGAGAAAAACAACACCACAACAATGGCTACTTTGAATGTTTTCTCTCAAACATTGCACCCTACTATTTCATCTTCTTTCTGTTCTTCTTCTTCTTCTTATTCAAATTCTAACTATGGTTCTTTCTGTCCAAATTCTAACACTTCTAAGTTTCATTTCTTTTCCAATTCTTCTTCCCTTTCTGCCCCTACCCTTTCTAACTCTTCTAAGACCCTTTTCACAAAGTCAGGTAGTGGAGGTTCATGGCTGCAAAGTAGTTCGGCATCTGTCAGCAACGAGCAAAAGAACGGTCCTGTCTATTCAGTATTTCCGTCATCGCCTGCTCAAGTTTCCTCTGTGCAAGACCTGTACAAATTTATTTGCTCAGGACCTTTGCTTGACAGAATTGGTTTGACGCCAGAGAACGTGGCCGAGTCTATAGATAAGTGGTTAACCTACGGGCGACAACTATGCAGACTGTTTCAGCTTAACGAATTGTTCTTGACCGAGCCTCAAAAAGTTAGGCTTTATCATTACTATATACCGGTCTTCCTCTGGTGCGAAAGTGAGATTGTTCAGCATCGGTCCAAATTCAAAGACGGAGAAGATATACCTCCTTTAGTGATTGGATTTAGTGCTCCTCAAGGCTGTGGAAAGACGACCCTCGTCTTTGCTCTCGACTATCTTTTCCAAATGATTGGCAGGAAGTCTGCAACAATATCGATAGATGATTTTTATTTGACGGCTGAAGGTCAGAATAAACTAAGAGAAGCTAATCCAGGAAATGCACTTCTTGAGTTTCGTGGAAATGCAGGAAGCCATGATCTTCCTATATCTGTTGAAACTCTGTCGGCTTTAACCAAAATGTCGAAAGAAGGTATCAAGATGAAGTTACCAAGATATGATAAATCTGCTTTCGGTGGGAGAGGTGACCGCGCTGATCCTTCAACATGGCCTGAAGTTGAAGGGCCACTTGAAGTTGTGTTATTTGAAGGCTGGATGCTTGGTTTCAAGCCCGTTCCAGTTGATGCTGTAAAATCGGTTGATCCTCAGCTGGAAATAATAAATAAGAACCTCGAAGCTTATTATGATGCGTGGGACAAATATATAAAGTCATGGATAGTCATTAAGATTAAGGATCCAAGTTGTGTCTACCAGTGGCGCTTGCAGGCTGAGATTGCGATGAGGGAGGCAGGAAATCCTGGAATGTCTGATGATGAGGTGAATGATTTTGTTTCGCGCTACCTGCCAGCATACAACGCGTACCTTCCTACACTTTACTCGCAGGGTCCGAATGGATCAGATCCTCAACATCTACTAACCATTGAAATTGATGAAAAGAGAAACCCGATCCTAGGTGTCTAGTTTAACTAGATGACGATAGGACAATGTTGGGTTTTTTCCATTCTTTGAGAGAATCGAGATGATCGTTCGGCACTCGTGGAGGAGACAAGTCAATGTATCAGCACTATTTTCTTTGAAATGTATAGAGGATATTCTTATCAGAACATAATAATAATAATAATAATGCCTATTCCATTAACTTTT
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5173
PRIMER_LEFT_PENALTY=0.263824
PRIMER_RIGHT_PENALTY=0.253482
PRIMER_LEFT_SEQUENCE=GCGGCTAAGATTTGTTTCTGAC
PRIMER_RIGHT_SEQUENCE=TAGGGTGCAATGTTTGAGAGAA
PRIMER_LEFT=139,22
PRIMER_RIGHT=245,22
PRIMER_LEFT_TM=60.264
PRIMER_RIGHT_TM=59.747
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=0.5654
PRIMER_LEFT_1_PENALTY=0.263824
PRIMER_RIGHT_1_PENALTY=0.301624
PRIMER_LEFT_1_SEQUENCE=GCGGCTAAGATTTGTTTCTGAC
PRIMER_RIGHT_1_SEQUENCE=CAGAAAGGGAAGAAGAATTGGA
PRIMER_LEFT_1=139,22
PRIMER_RIGHT_1=365,22
PRIMER_LEFT_1_TM=60.264
PRIMER_RIGHT_1_TM=59.698
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=227
PRIMER_PAIR_PENALTY_2=0.6090
PRIMER_LEFT_2_PENALTY=0.263824
PRIMER_RIGHT_2_PENALTY=0.345221
PRIMER_LEFT_2_SEQUENCE=GCGGCTAAGATTTGTTTCTGAC
PRIMER_RIGHT_2_SEQUENCE=CCTGACTTTGTGAAAAGGGTCT
PRIMER_LEFT_2=139,22
PRIMER_RIGHT_2=411,22
PRIMER_LEFT_2_TM=60.264
PRIMER_RIGHT_2_TM=59.655
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.6356
PRIMER_LEFT_3_PENALTY=0.603210
PRIMER_RIGHT_3_PENALTY=0.032347
PRIMER_LEFT_3_SEQUENCE=CTGGTGTAAATCACTGGTTTGG
PRIMER_RIGHT_3_SEQUENCE=TTCAAAGTAGCCATTGTTGTGG
PRIMER_LEFT_3=89,22
PRIMER_RIGHT_3=219,22
PRIMER_LEFT_3_TM=59.397
PRIMER_RIGHT_3_TM=60.032
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=0.6452
PRIMER_LEFT_4_PENALTY=0.603210
PRIMER_RIGHT_4_PENALTY=0.041981
PRIMER_LEFT_4_SEQUENCE=CTGGTGTAAATCACTGGTTTGG
PRIMER_RIGHT_4_SEQUENCE=AGTAGCCATTGTTGTGGTGTTG
PRIMER_LEFT_4=89,22
PRIMER_RIGHT_4=214,22
PRIMER_LEFT_4_TM=59.397
PRIMER_RIGHT_4_TM=59.958
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_03767 Ps_contig_mira-and-tgicl-ass_franssen_30978
SEQUENCE=CGGGGTATACACAAATACAATGTTTAGATAGATAGAATAGAATAATAGAATAGAATAGAATATAGTTGTACTATATTATTTTATTTTTTCTGGTGTAAATCACTGGTTTGGCCAAAGAACACGAGCCAAAGATTACGTGGCGGCTAAGATTTGTTTCTGACTTTTTGTTGCAAACTAGATAGATAGAGAAAAACAACACCACAACAATGGCTACTTTGAATGTTTTCTCTCAAACATTGCACCCTACTATTTCATCTTCTTTCTGTTCTTCTTCTTCTTCTTATTCAAATTCTAACTATGGTTCTTTCTGTCCAAATTCTAACACTTCTAAGTTTCATTTCTTTTCCAATTCTTCTTCCCTTTCTGCCCCTACCCTTTCTAACTCTTCTAAGACCCTTTTCACAAAGTCAGGTAGTGGAGGTTCATGGCTGCAAAGTAGTTCGGCATCTGTCAGCAACGAGCAAAAGAACGGTCCTGTCTATTCAGTATTTCCGTCATCGCCTGCTCAAGTTTCCTCTGTGCAAGACCTGTACAAATTTATTTGCTCAGGACCTTTGCTTGACAGAATTGGTTTGACGCCAGAGAACGTGGCCGAGTCTATAGATAAGTGGTTAACCTACGGGCGACAACTATGCAGACTGTTTCAGCTTAACGAATTGTTCTTGACCGAGCCTCAAAAAGTTAGGCTTTATCATTACTATATACCGGTCTTCCTCTGGTGCGAAAGTGAGATTGTTCAGCATCGGTCCAAATTCAAAGACGGAGAAGATATACCTCCTTTAGTGATTGGATTTAGTGCTCCTCAAGGCTGTGGAAAGACGACCCTCGTCTTTGCTCTCGACTATCTTTTCCAAATGATTGGCAGGAAGTCTGCAACAATATCGATAGATGATTTTTATTTGACGGCTGAAGGTCAGAATAAACTAAGAGAAGCTAATCCAGGAAATGCACTTCTTGAGTTTCGTGGAAATGCAGGAAGCCATGATCTTCCTATATCTGTTGAAACTCTGTCGGCTTTAACCAAAATGTCGAAAGAAGGTATCAAGATGAAGTTACCAAGATATGATAAATCTGCTTTCGGTGGGAGAGGTGACCGCGCTGATCCTTCAACATGGCCTGAAGTTGAAGGGCCACTTGAAGTTGTGTTATTTGAAGGCTGGATGCTTGGTTTCAAGCCCGTTCCAGTTGATGCTGTAAAATCGGTTGATCCTCAGCTGGAAATAATAAATAAGAACCTCGAAGCTTATTATGATGCGTGGGACAAATATATAAAGTCATGGATAGTCATTAAGATTAAGGATCCAAGTTGTGTCTACCAGTGGCGCTTGCAGGCTGAGATTGCGATGAGGGAGGCAGGAAATCCTGGAATGTCTGATGATGAGGTGAATGATTTTGTTTCGCGCTACCTGCCAGCATACAACGCGTACCTTCCTACACTTTACTCGCAGGGTCCGAATGGATCAGATCCTCAACATCTACTAACCATTGAAATTGATGAAAAGAGAAACCCGATCCTAGGTGTCTAGTTTAACTAGATGACGATAGGACAATGTTGGGTTTTTTCCATTCTTTGAGAGAATCGAGATGATCGTTCGGCACTCGTGGAGGAGACAAGTCAATGTATCAGCACTATTTTCTTTGAAATGTATAGAGGATATTCTTATCAGAACATAATAATAATAATAATAATGCCTATTCCATTAACTTTT
TARGET=266,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2247
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.192310
PRIMER_LEFT_SEQUENCE=CCACAACAATGGCTACTTTGAA
PRIMER_RIGHT_SEQUENCE=ACTACTTTGCAGCCATGAACCT
PRIMER_LEFT=198,22
PRIMER_RIGHT=439,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=60.192
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.2343
PRIMER_LEFT_1_PENALTY=0.041981
PRIMER_RIGHT_1_PENALTY=0.192310
PRIMER_LEFT_1_SEQUENCE=CAACACCACAACAATGGCTACT
PRIMER_RIGHT_1_SEQUENCE=ACTACTTTGCAGCCATGAACCT
PRIMER_LEFT_1=193,22
PRIMER_RIGHT_1=439,22
PRIMER_LEFT_1_TM=59.958
PRIMER_RIGHT_1_TM=60.192
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.3340
PRIMER_LEFT_2_PENALTY=0.032347
PRIMER_RIGHT_2_PENALTY=0.301624
PRIMER_LEFT_2_SEQUENCE=CCACAACAATGGCTACTTTGAA
PRIMER_RIGHT_2_SEQUENCE=CAGAAAGGGAAGAAGAATTGGA
PRIMER_LEFT_2=198,22
PRIMER_RIGHT_2=365,22
PRIMER_LEFT_2_TM=60.032
PRIMER_RIGHT_2_TM=59.698
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.3436
PRIMER_LEFT_3_PENALTY=0.041981
PRIMER_RIGHT_3_PENALTY=0.301624
PRIMER_LEFT_3_SEQUENCE=CAACACCACAACAATGGCTACT
PRIMER_RIGHT_3_SEQUENCE=CAGAAAGGGAAGAAGAATTGGA
PRIMER_LEFT_3=193,22
PRIMER_RIGHT_3=365,22
PRIMER_LEFT_3_TM=59.958
PRIMER_RIGHT_3_TM=59.698
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=173
PRIMER_PAIR_PENALTY_4=0.3668
PRIMER_LEFT_4_PENALTY=0.174509
PRIMER_RIGHT_4_PENALTY=0.192310
PRIMER_LEFT_4_SEQUENCE=ACAACACCACAACAATGGCTAC
PRIMER_RIGHT_4_SEQUENCE=ACTACTTTGCAGCCATGAACCT
PRIMER_LEFT_4=192,22
PRIMER_RIGHT_4=439,22
PRIMER_LEFT_4_TM=59.825
PRIMER_RIGHT_4_TM=60.192
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_03768 Ps_contig_mira-and-tgicl-ass_franssen_30978
SEQUENCE=CGGGGTATACACAAATACAATGTTTAGATAGATAGAATAGAATAATAGAATAGAATAGAATATAGTTGTACTATATTATTTTATTTTTTCTGGTGTAAATCACTGGTTTGGCCAAAGAACACGAGCCAAAGATTACGTGGCGGCTAAGATTTGTTTCTGACTTTTTGTTGCAAACTAGATAGATAGAGAAAAACAACACCACAACAATGGCTACTTTGAATGTTTTCTCTCAAACATTGCACCCTACTATTTCATCTTCTTTCTGTTCTTCTTCTTCTTCTTATTCAAATTCTAACTATGGTTCTTTCTGTCCAAATTCTAACACTTCTAAGTTTCATTTCTTTTCCAATTCTTCTTCCCTTTCTGCCCCTACCCTTTCTAACTCTTCTAAGACCCTTTTCACAAAGTCAGGTAGTGGAGGTTCATGGCTGCAAAGTAGTTCGGCATCTGTCAGCAACGAGCAAAAGAACGGTCCTGTCTATTCAGTATTTCCGTCATCGCCTGCTCAAGTTTCCTCTGTGCAAGACCTGTACAAATTTATTTGCTCAGGACCTTTGCTTGACAGAATTGGTTTGACGCCAGAGAACGTGGCCGAGTCTATAGATAAGTGGTTAACCTACGGGCGACAACTATGCAGACTGTTTCAGCTTAACGAATTGTTCTTGACCGAGCCTCAAAAAGTTAGGCTTTATCATTACTATATACCGGTCTTCCTCTGGTGCGAAAGTGAGATTGTTCAGCATCGGTCCAAATTCAAAGACGGAGAAGATATACCTCCTTTAGTGATTGGATTTAGTGCTCCTCAAGGCTGTGGAAAGACGACCCTCGTCTTTGCTCTCGACTATCTTTTCCAAATGATTGGCAGGAAGTCTGCAACAATATCGATAGATGATTTTTATTTGACGGCTGAAGGTCAGAATAAACTAAGAGAAGCTAATCCAGGAAATGCACTTCTTGAGTTTCGTGGAAATGCAGGAAGCCATGATCTTCCTATATCTGTTGAAACTCTGTCGGCTTTAACCAAAATGTCGAAAGAAGGTATCAAGATGAAGTTACCAAGATATGATAAATCTGCTTTCGGTGGGAGAGGTGACCGCGCTGATCCTTCAACATGGCCTGAAGTTGAAGGGCCACTTGAAGTTGTGTTATTTGAAGGCTGGATGCTTGGTTTCAAGCCCGTTCCAGTTGATGCTGTAAAATCGGTTGATCCTCAGCTGGAAATAATAAATAAGAACCTCGAAGCTTATTATGATGCGTGGGACAAATATATAAAGTCATGGATAGTCATTAAGATTAAGGATCCAAGTTGTGTCTACCAGTGGCGCTTGCAGGCTGAGATTGCGATGAGGGAGGCAGGAAATCCTGGAATGTCTGATGATGAGGTGAATGATTTTGTTTCGCGCTACCTGCCAGCATACAACGCGTACCTTCCTACACTTTACTCGCAGGGTCCGAATGGATCAGATCCTCAACATCTACTAACCATTGAAATTGATGAAAAGAGAAACCCGATCCTAGGTGTCTAGTTTAACTAGATGACGATAGGACAATGTTGGGTTTTTTCCATTCTTTGAGAGAATCGAGATGATCGTTCGGCACTCGTGGAGGAGACAAGTCAATGTATCAGCACTATTTTCTTTGAAATGTATAGAGGATATTCTTATCAGAACATAATAATAATAATAATAATGCCTATTCCATTAACTTTT
TARGET=1671,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03769 Ps_contig_mira-and-tgicl-ass_franssen_30980
SEQUENCE=ATTATTTGAGAGATTATTCATATGCCTCAAATCAAAATGACATACTGTCATAGTTTAAAATGGGAAAACTGAGTTTCATACTAGCATCTCCAAAAGATTTAGCTTCACCTTGATCATACAAAATTCAACTCATGTTCCAACTTTATATGCTCCCGAATGCATAAAACCGACTATTACATGCGAATCACTAGTCTAACATCTAAAACAATTTATTGCAAGTGTACACCAACAATGCTCAGACCAATTTATCATCTAACCAGCTCTACTTGCCAAGTACATGGAGCATTATAGCGTTCTGTGCATGCATTCGGTTCTCAGCTTGAGGGAAGACTATTGAATATGGAGCTTCAACAACTTCGTCAGTCACCTCCACCCCTCTTTCCGCTGGTAAGCAGTGCATGAAAAATGCCTTGGAACCAGCCGCATCCATAAGATTCTGATCCACCTGAAATCCTTTAAACGCTTCACGACGATATGCAGCTTCTTCCTTTTGCCCCATGCTGGCCCAGACATCTGAATACACAACATCAGCTCCTCTAACAGCTTCTTTCGGATCATGACTTATCTCAATCTTGCTGATTCCAGCCTTCCTTGCCTTCTCAACAGTTTTCGCATCAGGTTCAAAACCTTTTGGACACGCACAAACAAAGTGAAAAGGAACTACAGCTGCCAACAACAACCATGAGTGGACAATATTATTCCCATCTCCAACATAGACAACCTTGGTTCCTTCAAACCGACCAATGTGCTCAATCATAGTAAGGGCATCAGCCATTATCTGGACGGGATGGTTGTAGTCTGTCAAACCATTGATGACAGGGACACTCGCATACTTTGCAAGATCAAGTATGTCCTGATGAGAAAAAACACGGGCCATAATGATATCGTTATAGCGAGACAAAACTCGAGCAACATCACGAGTCTCCTCTCGCTTCCCCATTTGAATGTCATTGGGTCCTAAGTAAATAGCATGACCACCTAACAATGAAAAACCAGTCTCAAATGAAACCCTTGTTCTCATGGATGGCTTTGCAAATATCATCGACATTGTCTTCCCTTCAAAAGGCCGAAATGTCCTGTCCCCTGATTTCAACAATGTCTTCACCTCAATAGCTCGATCTAATATCTTCAAAATAGTTTCCTTGTCAAAATCATCTATGTGAATAAAATCTTTCAACCCATTTCCAACTTTCGCGGTGAGAGTAGATTCGGCGGCGGGAGCGGAAGAGGCTTGGCATGAGATTCTTCTGAGTGGTGAAGGCGACGACGACGAAGAAGAAGAAAGAGAAACGGAAGGGGAATGGCGGAAATTGTGAGATGAGGGAGAAAGGTAGGGTTTGTGTGATCCAACGGTGGTGAAACAGTAACAGTGGGCAGTGATGACACCCATTTTCTCTTTACTTGGGATGTTTTATTCTTAGCTTCCTCAGTTACACTCCAAACACCACCATAGTATAATGTTGTTTCTCAATTTATACCAACCACCCTATCATAGCAGGTTATTGTTCTTGTT
TARGET=1262,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2774
PRIMER_LEFT_PENALTY=0.167242
PRIMER_RIGHT_PENALTY=0.110131
PRIMER_LEFT_SEQUENCE=ATCATCGACATTGTCTTCCCTT
PRIMER_RIGHT_SEQUENCE=ACCTTTCTCCCTCATCTCACAA
PRIMER_LEFT=1037,22
PRIMER_RIGHT=1331,22
PRIMER_LEFT_TM=59.833
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=0.3203
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=0.099308
PRIMER_LEFT_1_SEQUENCE=CCCCTGATTTCAACAATGTCTT
PRIMER_RIGHT_1_SEQUENCE=CACACAAACCCTACCTTTCTCC
PRIMER_LEFT_1=1080,22
PRIMER_RIGHT_1=1343,22
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.3311
PRIMER_LEFT_2_PENALTY=0.220992
PRIMER_RIGHT_2_PENALTY=0.110131
PRIMER_LEFT_2_SEQUENCE=CCCCTGATTTCAACAATGTCTT
PRIMER_RIGHT_2_SEQUENCE=ACCTTTCTCCCTCATCTCACAA
PRIMER_LEFT_2=1080,22
PRIMER_RIGHT_2=1331,22
PRIMER_LEFT_2_TM=60.221
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=0.3467
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.125755
PRIMER_LEFT_3_SEQUENCE=CCCCTGATTTCAACAATGTCTT
PRIMER_RIGHT_3_SEQUENCE=ACAAACCCTACCTTTCTCCCTC
PRIMER_LEFT_3=1080,22
PRIMER_RIGHT_3=1340,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.874
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.3538
PRIMER_LEFT_4_PENALTY=0.254537
PRIMER_RIGHT_4_PENALTY=0.099308
PRIMER_LEFT_4_SEQUENCE=GATTCTTCTGAGTGGTGAAGGC
PRIMER_RIGHT_4_SEQUENCE=CACACAAACCCTACCTTTCTCC
PRIMER_LEFT_4=1240,22
PRIMER_RIGHT_4=1343,22
PRIMER_LEFT_4_TM=60.255
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=104
=
PRIMER_SEQUENCE_ID=SSR_03770 Ps_contig_mira-and-tgicl-ass_franssen_30982
SEQUENCE=GTAAAAAAAATCTCTGAATCTTGTGCATAAAGTTAATTAGAAAAACTNTTGACATCCAAAATCTCATTTTCTCAAGTAAAACCGAATACAAATAATAAAATTGTAACATGCATTTAGAAGAAAAAATCATTACAATATTTCTTCTCAAGCGAAAACTTGTAGCAACGTTTTGCTAATCAAAGTATACCAAAAATATTGGTAATAATCAAGCACAAGCTTTAAGTATCTAGGAAGGAGACCTAAACTTGAAGTACCAATAAATCACACCACCACCACTGACTATATAACACCATCTCTCCAAAAGGAGAGGGACAAAAAGGGGCAAGAGACTCAATAAATTCAGACTAAAATAAAACTAAGGGGAGGAAAATGAATTAAGGACCATGAAAAACCACATGAAATTGAGAACTCTTTTTTCATCAGGCGCTAGGACCCCAAAGCAACTTTCAGTAGCATCATACTCTGAATAAATAAACTACTGCTGCTTGCACTCTGGTGGTCTTTCACCCTGCTGACCATCGCCAAGCTGCGCAATTCCGCTCTTCTTACTAGCAGATGACTTCTTTTTAGTCTTAGTCTCTTCCTCTTCTTCATCATCATCATCTTCGTCCTCATCCTCCTCTTCATCATCCTCCTCAGCATCATCGTCCTCATCCTCATCTTCATCATCCAGGTCTCCAAACTCCTCTCCCTGAGCAGCCTCCCCAGTAAACCACGACACAGCAGATGATCTTATCTCTTATTGTTGACCCAATGTCATAGTCTTGTTCCATCTGATTCTGAAGTTCCTCAGCCATATCTTCATCAATGTCTTCATCATCTTCAGGGACTTCTGGTGGATTGAAAAAGTTAAAGAAACTTTCACAGGTTTCAGTTTTGGTAATTGGTTTAGCATTCTTTGCACCCTTCTTGGGCTTTTTCTTCAAAACCTTTTGGGTCAGGCATTTTCCTGGAAGCCACTGAATTTCAGTCCCAATAGCCTTCTCCAGTATAGGCTCATCCTCATCAACCATATGATAGATTTTGGTCAACACCGAGTTTGAAAAATAGGGATTGGAATCGAAGAAAAACTCAAGTTTGAATCCTTTAGGTTCTTCGATCCTTGTCCACTTGATATCTTTGAGAAACTTGAGAGCGCCTTCATCGCGCTCTGTAATTTCTTCACCTAAGACATCATTGTTTTTCATAGCATTAAGCCAAAATGAAGGCACTCCTTTCTCTTTATCCTCTTCTGCAACAGCAGTTTCAACTGCTACCCCTTCCACTTCAGCAACACCATTCACAATATCATAACGCTTAGTGTACAATGGTTGATACAAAATTTGGTATTTGGCTTCAAGAGCAACTCTCTCTTCCAAAAACTTTGCCTCCAATTCATCATGTTCACCCTGAATCTCTCTGAGAACCTCCACACGCTTCCTGACAACAGGAGACAGACTCTCCAGAACATCAGAGTGTTGCCCAGCAAGACTCTGTATCTTACTCTTGAGAGCGTTGACCAGGTCAGCCCGATCCTCTTCATTGAGAGCGGAACTGAGATCTGAGATGTTGAAAGCTTCCTTGTTGTTCGTCATTGAGAAGTGAGAAATGGAGGAAGAAGAAGATGACAGCAAAAAAGCAAAACCTAGCCGGAAGAAGGGGAGGGTTTTGATTGATTGTACTGTCTGGAACTGGCTCTCTAATCCTCTATGTTCGCTGTCNNCTANNANCCCNNCTCCCTCTCTT
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2964
PRIMER_LEFT_PENALTY=0.186309
PRIMER_RIGHT_PENALTY=0.110131
PRIMER_LEFT_SEQUENCE=TCTCAAGCGAAAACTTGTAGCA
PRIMER_RIGHT_SEQUENCE=TCTCCTTTTGGAGAGATGGTGT
PRIMER_LEFT=142,22
PRIMER_RIGHT=307,22
PRIMER_LEFT_TM=60.186
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.4253
PRIMER_LEFT_1_PENALTY=0.186309
PRIMER_RIGHT_1_PENALTY=0.238974
PRIMER_LEFT_1_SEQUENCE=TCTCAAGCGAAAACTTGTAGCA
PRIMER_RIGHT_1_SEQUENCE=CTCTCCTTTTGGAGAGATGGTG
PRIMER_LEFT_1=142,22
PRIMER_RIGHT_1=308,22
PRIMER_LEFT_1_TM=60.186
PRIMER_RIGHT_1_TM=60.239
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.4939
PRIMER_LEFT_2_PENALTY=0.186309
PRIMER_RIGHT_2_PENALTY=0.307639
PRIMER_LEFT_2_SEQUENCE=TCTCAAGCGAAAACTTGTAGCA
PRIMER_RIGHT_2_SEQUENCE=CCTTAATTCATTTTCCTCCCCT
PRIMER_LEFT_2=142,22
PRIMER_RIGHT_2=379,22
PRIMER_LEFT_2_TM=60.186
PRIMER_RIGHT_2_TM=59.692
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.5705
PRIMER_LEFT_3_PENALTY=0.186309
PRIMER_RIGHT_3_PENALTY=0.384229
PRIMER_LEFT_3_SEQUENCE=TCTCAAGCGAAAACTTGTAGCA
PRIMER_RIGHT_3_SEQUENCE=CTCCTTTTGGAGAGATGGTGTT
PRIMER_LEFT_3=142,22
PRIMER_RIGHT_3=306,22
PRIMER_LEFT_3_TM=60.186
PRIMER_RIGHT_3_TM=59.616
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.6517
PRIMER_LEFT_4_PENALTY=0.186309
PRIMER_RIGHT_4_PENALTY=0.465395
PRIMER_LEFT_4_SEQUENCE=TCTCAAGCGAAAACTTGTAGCA
PRIMER_RIGHT_4_SEQUENCE=ATTGAGTCTCTTGCCCCTTTTT
PRIMER_LEFT_4=142,22
PRIMER_RIGHT_4=334,22
PRIMER_LEFT_4_TM=60.186
PRIMER_RIGHT_4_TM=60.465
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_03771 Ps_contig_mira-and-tgicl-ass_franssen_30982
SEQUENCE=GTAAAAAAAATCTCTGAATCTTGTGCATAAAGTTAATTAGAAAAACTNTTGACATCCAAAATCTCATTTTCTCAAGTAAAACCGAATACAAATAATAAAATTGTAACATGCATTTAGAAGAAAAAATCATTACAATATTTCTTCTCAAGCGAAAACTTGTAGCAACGTTTTGCTAATCAAAGTATACCAAAAATATTGGTAATAATCAAGCACAAGCTTTAAGTATCTAGGAAGGAGACCTAAACTTGAAGTACCAATAAATCACACCACCACCACTGACTATATAACACCATCTCTCCAAAAGGAGAGGGACAAAAAGGGGCAAGAGACTCAATAAATTCAGACTAAAATAAAACTAAGGGGAGGAAAATGAATTAAGGACCATGAAAAACCACATGAAATTGAGAACTCTTTTTTCATCAGGCGCTAGGACCCCAAAGCAACTTTCAGTAGCATCATACTCTGAATAAATAAACTACTGCTGCTTGCACTCTGGTGGTCTTTCACCCTGCTGACCATCGCCAAGCTGCGCAATTCCGCTCTTCTTACTAGCAGATGACTTCTTTTTAGTCTTAGTCTCTTCCTCTTCTTCATCATCATCATCTTCGTCCTCATCCTCCTCTTCATCATCCTCCTCAGCATCATCGTCCTCATCCTCATCTTCATCATCCAGGTCTCCAAACTCCTCTCCCTGAGCAGCCTCCCCAGTAAACCACGACACAGCAGATGATCTTATCTCTTATTGTTGACCCAATGTCATAGTCTTGTTCCATCTGATTCTGAAGTTCCTCAGCCATATCTTCATCAATGTCTTCATCATCTTCAGGGACTTCTGGTGGATTGAAAAAGTTAAAGAAACTTTCACAGGTTTCAGTTTTGGTAATTGGTTTAGCATTCTTTGCACCCTTCTTGGGCTTTTTCTTCAAAACCTTTTGGGTCAGGCATTTTCCTGGAAGCCACTGAATTTCAGTCCCAATAGCCTTCTCCAGTATAGGCTCATCCTCATCAACCATATGATAGATTTTGGTCAACACCGAGTTTGAAAAATAGGGATTGGAATCGAAGAAAAACTCAAGTTTGAATCCTTTAGGTTCTTCGATCCTTGTCCACTTGATATCTTTGAGAAACTTGAGAGCGCCTTCATCGCGCTCTGTAATTTCTTCACCTAAGACATCATTGTTTTTCATAGCATTAAGCCAAAATGAAGGCACTCCTTTCTCTTTATCCTCTTCTGCAACAGCAGTTTCAACTGCTACCCCTTCCACTTCAGCAACACCATTCACAATATCATAACGCTTAGTGTACAATGGTTGATACAAAATTTGGTATTTGGCTTCAAGAGCAACTCTCTCTTCCAAAAACTTTGCCTCCAATTCATCATGTTCACCCTGAATCTCTCTGAGAACCTCCACACGCTTCCTGACAACAGGAGACAGACTCTCCAGAACATCAGAGTGTTGCCCAGCAAGACTCTGTATCTTACTCTTGAGAGCGTTGACCAGGTCAGCCCGATCCTCTTCATTGAGAGCGGAACTGAGATCTGAGATGTTGAAAGCTTCCTTGTTGTTCGTCATTGAGAAGTGAGAAATGGAGGAAGAAGAAGATGACAGCAAAAAAGCAAAACCTAGCCGGAAGAAGGGGAGGGTTTTGATTGATTGTACTGTCTGGAACTGGCTCTCTAATCCTCTATGTTCGCTGTCNNCTANNANCCCNNCTCCCTCTCTT
TARGET=591,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1507
PRIMER_LEFT_PENALTY=0.113753
PRIMER_RIGHT_PENALTY=0.036956
PRIMER_LEFT_SEQUENCE=AACTACTGCTGCTTGCACTCTG
PRIMER_RIGHT_SEQUENCE=ATGATGAAGAGGAGGATGAGGA
PRIMER_LEFT=473,22
PRIMER_RIGHT=629,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.1507
PRIMER_LEFT_1_PENALTY=0.113753
PRIMER_RIGHT_1_PENALTY=0.036956
PRIMER_LEFT_1_SEQUENCE=AACTACTGCTGCTTGCACTCTG
PRIMER_RIGHT_1_SEQUENCE=GATGATGAAGAGGAGGATGAGG
PRIMER_LEFT_1=473,22
PRIMER_RIGHT_1=630,22
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=0.1507
PRIMER_LEFT_2_PENALTY=0.113753
PRIMER_RIGHT_2_PENALTY=0.036956
PRIMER_LEFT_2_SEQUENCE=AACTACTGCTGCTTGCACTCTG
PRIMER_RIGHT_2_SEQUENCE=GGATGATGAAGAGGAGGATGAG
PRIMER_LEFT_2=473,22
PRIMER_RIGHT_2=631,22
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.1507
PRIMER_LEFT_3_PENALTY=0.113753
PRIMER_RIGHT_3_PENALTY=0.036956
PRIMER_LEFT_3_SEQUENCE=AACTACTGCTGCTTGCACTCTG
PRIMER_RIGHT_3_SEQUENCE=AGGATGATGAAGAGGAGGATGA
PRIMER_LEFT_3=473,22
PRIMER_RIGHT_3=632,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=60.037
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=0.1507
PRIMER_LEFT_4_PENALTY=0.113753
PRIMER_RIGHT_4_PENALTY=0.036956
PRIMER_LEFT_4_SEQUENCE=AACTACTGCTGCTTGCACTCTG
PRIMER_RIGHT_4_SEQUENCE=GAGGATGATGAAGAGGAGGATG
PRIMER_LEFT_4=473,22
PRIMER_RIGHT_4=633,22
PRIMER_LEFT_4_TM=59.886
PRIMER_RIGHT_4_TM=60.037
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_03772 Ps_contig_mira-and-tgicl-ass_franssen_30983
SEQUENCE=AGCGCCTGATAAAAAAAGAGTTCTCAATTTCATGTGGTTTTTCATGGTCCTTAATTCATTTTCCTCCCCTTAGTTTTATTTTAGTCTGAATTTATTGAGTCTCTTGCCCCTTTTTGTCCCTCTCCTTTTTGGAGAGATGGTGTTATATAGTCAGTGGTGGTGGTGTGATTTATTGGTACTTCAAGTTTAGGTCTCCTTCCTAGATACTTAAAGCTTGTGCTTGATTATTACCAATTATTTTTRGTATTACTTTGATTAGACAAAACGTTGCTACTAAGTTTTCGCTTAGAAGTAAGAAATATTGTAATGTTATTTTTTTCTTACTAAATGCATGTTAACTAATTTTATTATTTGTTATTTCGGTTTTAACTAA
TARGET=154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03773 Ps_contig_mira-and-tgicl-ass_franssen_30984
SEQUENCE=CTAAAATAGAGAGCAAACATNCNTNTTTGAATGCAATAACTTGACTCACTTGCCACTTGCTACAACAATTATTGGATTTTTGGTAACAAACATACATTCACACACGACACGTTTTCTCATTCCATGTTTTGCCCCATCATTACACTCCACACCAAAAAATCTCATTTATATTCATACTTTATAAGAAAATTATTTGGTTTCCTCATGTGATGTCTATATACATACTCTACAAAAGAATTCAAATTCAATGTCTGCATCATCATCATGTATTATACATCCACAGCATCAACAGGAAGTGAAACACTTCTGTGTAGAGTCTCTTGAAGCAACAGCCTTCTGTAGAAACTCTTTGCAGTAGCCATATTTTCTTCGTCGCAATACACCGCGCCGCAATTTTGAGCCCTTCGGGTCTCCAAATCGGGTTGGCTCTTGTGCTCTCGAGAATCGTAATCCCTGTAATTATCGAATCCGTTTACTGTTTCTGATGTTGTTACGTGCTTTATGTTGCTGCTCGTATGCGGCGATTTTGGTTCTAGTCCTATTTGTTTATCCCTAAGCTCCCTTAACTCCATTTGATATTTTGCTTTCAGCCATCTCAACTCGTGTTGTATCTCTTTCTCATAATCATTCTCGACTCCGCAATGTAGTGAACCGATGGTAGAATGGTTTCCTAGTTCGTAATGTTCGTCTGAAGGGCTTCGAGTAGACTCTACTGGACTCAGTTCGCGGCTTTCTTGATGATGACTCCACGATTCTTGATACTGTAAAAAGTCGGATTCGCTTGATATGTTAGGCGCTTCCTCGCTGACGTGGTTGTCGTACTCCTCGGATTGGAATGTAATCTCTTCGAAACGGCCATGCATTGAAGCACATCCATGCATGCAGCATTGAAGAAGCTGCAAGTTTTTTCCACTTAGATTACGGGAAAGAAAATCCGTAACTGTAGTATGATTAGAAACACAATTTTCGCAGAAACTTTGGTTTTCATAGTTGGCGGTTTCCTCGATTCCCGGTCCTCGCCTCCATTTTGGTACCAACGACGCAATTTCTCCGTCTATCATACCGGCTATTCTGGTAACATCTTGATCAGTAATGTCGAGTTCCGCAACCATTTCGGTTGCTACACTTAATGCTGTATCCAATTCTATGTCAAATGGGAAATAAATGTTTCGGATACGACCTTCTTTGTCTGCGATTCTTAGTCTCAAAAAGATGCCACCGTCGTCTTTCCTCTTGCCTTTGATGCTTATGTCAACATCTTTGGAGGTTTCATCGTCTTGGCACTCGAAAAGTTCAATTCCGTGTTCAATCTCAGCCGGATGAGAATAACAATCTGCTTCATACCCGAAACCATTTGAGTATTCGGTACTTATGTTACTATAGCTACGGTCGAGTGAAAAGAATGACTGACTAACAAGAGGACCCAAGTCAACAAACTCTTCAACATCTACCGATCCCAAATTATATTCGTAATCATCAATTCGAAGAAAAGGGTCATCAAGAAGCTCCCTCGCAGAGAGCCTTAGAGATACAGTTGCGAGACATTTCTCAACAAACTCTCGCGTTTCAGGATCTTTCACTTTATACAAAGCGTCTGGTTTTTTCCCAGAAGAAACTTTCTTGTAGATTTGTGCCGGATGGCTGCATTCGCTATATGGATATTCAAATGTGACCATTTCTAACACACACATACCAAAGGAATATATGTCAACTAATTCATTATACTCCTCTTCATATACTTCTGGTGCCATGAACTCGGGCGTTCCTACACAATGAGCAGCATGTGATTTCCGTAGAATCGCAGCCAAACCAAGATCCCCAATCTTAACTTCCCCTTGATTACCATTAATGAAAATATTATCACATTTAAGATCTCTATGAATCACAGGTGGATCATGGCTATGAAGATATAAAAGTCCTTGCAGGATCTGCCTACACCAATGCTTCACTGCTCTTATGTTAACCTTCTTGTGCTTTAACCTATACTGTCTAAGAGTACCGGAAGTAAAGAATTCAGTGACAAAGTTGATATTCCTATTGGAAGTATCAACCCAAGAAGTGTAGAATTTCATTATGTTTCTGTGTTTCAATGTCTTGAGAAGATGAATTTCACAGTAAAGCCTCTCAAGATCTTCAGGATTTTGCAGAAAATCATATAGTTTGATTTGATTCCAAGCAACTTCAATCCCTTGATACTCATCAAATGCTCTRTANACTGTTTTAGAAGCTCCTTTACCGAGAATTTCATTGTATCTGCCATATCTACCGGTTGGATCAACTTCAACAAAATCTGAGTCATCATCTGTTTCAAGGTGYGTACACCATTCATTCTTCTGTTTCA
TARGET=255,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03774 Ps_contig_mira-and-tgicl-ass_franssen_30987
SEQUENCE=ACTAATTTTTTTTNAAACTATAAATGTCTTCTTTATACCTTTATAAGAAAGCAGGAAATTCACAGCTGATCCAATAAAATGGAAGAGATAATTAAAAATAGGTAAATTCGGAAGATCCATCATGATATGCAGACTATAATAAAATTAATTTTTTTGGGTACATGTGATGAAATTCGATAGCGCTTGAAATTCACATCATTCGGCAGAGTGGGAAGGTAACAGCTGCTTAGGAACATGATAATCACATCACCATGAGCAACAGCTACTAGTTGATGCATCAGACTCTTGGGGTTTCTGCTTGAAGATGTTCTTTTTCACACCAGATGAGCCCTCGGCCAAGAGACTGGGTGTATCCAAAATCTTCATTACAAGCTCCTCAAAACACTGCTCAACATTTACTCGAGTTTTTGCACTGCATTCAGTATAAAGGCATCCATATTGCCTTGCAAAGTCCACCGCTTCCTTTTTGGTAACAACCCTTTCACTTTCCTTGTCAACTTTGTTCCCGACAAGCATCTTGATGCAATCTTGATTTGTTGAGTATAGGTCAATTTCTTTAGCCCATATGTCAGATAGATTTGTAAAGGTATCCCGCCGCGTTACATCGTATACCATAATTATTCCTTGTGCACCTCTATAATATGAACCTGTAAGTGTTCTAAACCTTTCCTGTCCAGCTGTGTCCCAAATAGCAAGTTTTAACTTTTTTCCACCAAGTGTAACATATTTAACTTTGAAGTCTACACCGATGGTGGGAGAAAGATCCTCGAAGTTATCAGAAGTGAATCTGAGAAGCAGCGTGCTTTTTCCAACTCCAGAATCACCAATCATGAGAAGCTTGAACAAGTAATCGAATTCAGTGTGAGTCGAGGAAGAGGAAGAAACATCCATTTGGAACGGTGCAAATAAGCACGGTTAGGTTTAAGCTATTTTGAGTGGTTGTTTTTTCTTTTCTTTTCCTTTCCTTCTGTTTGTTGGGAGAATCTAGACTTGTGTTGAATGAATTCAATGGAAGAAAAGTATATTTAGAAGGTTGCCGAAAGTCAAAGAAAGGTTCAAGGTTGATCTATGCTTTACTTCCTGCGGTGAA
TARGET=945,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0291
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=GTGAGTCGAGGAAGAGGAAGAA
PRIMER_RIGHT_SEQUENCE=ATTCTCCCAACAAACAGAAGGA
PRIMER_LEFT=861,22
PRIMER_RIGHT=983,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=0.0291
PRIMER_LEFT_1_PENALTY=0.005574
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=GTGAGTCGAGGAAGAGGAAGAA
PRIMER_RIGHT_1_SEQUENCE=GATTCTCCCAACAAACAGAAGG
PRIMER_LEFT_1=861,22
PRIMER_RIGHT_1=984,22
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.0910
PRIMER_LEFT_2_PENALTY=0.067436
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=CCAGAATCACCAATCATGAGAA
PRIMER_RIGHT_2_SEQUENCE=ATTCTCCCAACAAACAGAAGGA
PRIMER_LEFT_2=814,22
PRIMER_RIGHT_2=983,22
PRIMER_LEFT_2_TM=59.933
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.0910
PRIMER_LEFT_3_PENALTY=0.067436
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=CCAGAATCACCAATCATGAGAA
PRIMER_RIGHT_3_SEQUENCE=GATTCTCCCAACAAACAGAAGG
PRIMER_LEFT_3=814,22
PRIMER_RIGHT_3=984,22
PRIMER_LEFT_3_TM=59.933
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.1969
PRIMER_LEFT_4_PENALTY=0.173354
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=TCGAGGAAGAGGAAGAAACATC
PRIMER_RIGHT_4_SEQUENCE=GATTCTCCCAACAAACAGAAGG
PRIMER_LEFT_4=866,22
PRIMER_RIGHT_4=984,22
PRIMER_LEFT_4_TM=59.827
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_03775 Ps_contig_mira-and-tgicl-ass_franssen_30992
SEQUENCE=CTAACCCARNGTACGNGGGGCACTCCTCACAGCATATACTCATTCATTCATTCACTCACTCACATATAACTCAATATTGAAAGTGAGAGAATACAAAGAAACAAAAAATGGAAAACTTTCCAATTGTTGACATGGGGAAGCTTAACACAGAAGACAGAAAATCAACCATGGAGTTGATCAAAGATGCCTGTGAGAACTGGGGTTTCTTTGAGTGTGTGAATCATGGTATATCTATTGAGATGATGGATACGGTTGAGAAGCTGACAAAGGAACACTACAAGAAGTGTATGGAACAAAGGTTCAAAGAAATGGTTGCAACCAAAGGTTTGGAGTGTGTTCAGTCAGAGATAGATGATTTGGATTGGGAAAGCACTTTCTTTTTGCGCCATCTTCCTGTTTCCAGTATCTCAGAGATCCCAGATCTTGATGATGATTACAGGAAGGTGATGAAGGAATTTGCGTTGAAATTGGAAGAACTTGCGGAGGAACTTCTTGACTTGTTGTGTGAGAATCTTGGGCTTGAGAAAGGGTATTTGAAGAAGGCTTTTTATGGATCAAAGGGTCCAAATTTTGGGACGAAAGTTAGTAACTACCCTCCTTGTCCTAAACCGGAACTCATCAAGGGACTTAGAGCTCACACAGATGCCGGCGGAATCATTCTTCTCTTCCAAGATGACAAAGTCAGTGGACTTCAGCTTCTCAAAGATGACCAATGGATTGATGTCCCTCCAATGCGTCACTCTATTGTCATCAATCTTGGTGATCAACTCGAGGTGATAACCAATGGAAAGTACAAGAGTGTGATGCATAGAGTAATAGCACAAACAGATGGTGCTAGAATGTCCATAGCATCCTTCTACAATCCAGGGGATGATGCTGTGATTTCACCAGCTTCAACCTTATTGAAGGAAAATGAAACAAGTGAAGTTTATCCAAAGTTTGTGTTTGATGATTACATGAAACTCTACATGGGACTCAAGTTCCAAGCTAAAGAGCCTAGATTTGAAGCTATGATGAAAGCCATGTCAAGTGTTAAAGTGGGACCAGTAGTAAGCATATGATTATGTGTGTGGTTTATATTACTAAGGTATTATTAATGTTTGGTTGGTGTTTTAAGATTGTGCAGTGTCTAAAGTGTTATATTAAAAAAAATAAAGTATGATCTTAGATTGTGTGTGACAATTAATCAGTTATGATTAAGTGATTAAGTTTATGTAATGCGATGCAGTGATTTATAAAATTATAACTGTTTTTATAAAGCANAAAC
TARGET=40,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03776 Ps_contig_mira-and-tgicl-ass_franssen_30992
SEQUENCE=CTAACCCARNGTACGNGGGGCACTCCTCACAGCATATACTCATTCATTCATTCACTCACTCACATATAACTCAATATTGAAAGTGAGAGAATACAAAGAAACAAAAAATGGAAAACTTTCCAATTGTTGACATGGGGAAGCTTAACACAGAAGACAGAAAATCAACCATGGAGTTGATCAAAGATGCCTGTGAGAACTGGGGTTTCTTTGAGTGTGTGAATCATGGTATATCTATTGAGATGATGGATACGGTTGAGAAGCTGACAAAGGAACACTACAAGAAGTGTATGGAACAAAGGTTCAAAGAAATGGTTGCAACCAAAGGTTTGGAGTGTGTTCAGTCAGAGATAGATGATTTGGATTGGGAAAGCACTTTCTTTTTGCGCCATCTTCCTGTTTCCAGTATCTCAGAGATCCCAGATCTTGATGATGATTACAGGAAGGTGATGAAGGAATTTGCGTTGAAATTGGAAGAACTTGCGGAGGAACTTCTTGACTTGTTGTGTGAGAATCTTGGGCTTGAGAAAGGGTATTTGAAGAAGGCTTTTTATGGATCAAAGGGTCCAAATTTTGGGACGAAAGTTAGTAACTACCCTCCTTGTCCTAAACCGGAACTCATCAAGGGACTTAGAGCTCACACAGATGCCGGCGGAATCATTCTTCTCTTCCAAGATGACAAAGTCAGTGGACTTCAGCTTCTCAAAGATGACCAATGGATTGATGTCCCTCCAATGCGTCACTCTATTGTCATCAATCTTGGTGATCAACTCGAGGTGATAACCAATGGAAAGTACAAGAGTGTGATGCATAGAGTAATAGCACAAACAGATGGTGCTAGAATGTCCATAGCATCCTTCTACAATCCAGGGGATGATGCTGTGATTTCACCAGCTTCAACCTTATTGAAGGAAAATGAAACAAGTGAAGTTTATCCAAAGTTTGTGTTTGATGATTACATGAAACTCTACATGGGACTCAAGTTCCAAGCTAAAGAGCCTAGATTTGAAGCTATGATGAAAGCCATGTCAAGTGTTAAAGTGGGACCAGTAGTAAGCATATGATTATGTGTGTGGTTTATATTACTAAGGTATTATTAATGTTTGGTTGGTGTTTTAAGATTGTGCAGTGTCTAAAGTGTTATATTAAAAAAAATAAAGTATGATCTTAGATTGTGTGTGACAATTAATCAGTTATGATTAAGTGATTAAGTTTATGTAATGCGATGCAGTGATTTATAAAATTATAACTGTTTTTATAAAGCANAAAC
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03777 Ps_contig_mira-and-tgicl-ass_franssen_30993
SEQUENCE=AACACTTTCGTTGAAATAAAAAACTCTCCGTTCACCTCTTCATCGTTGTTCTGTCTCTCCTTCTTTCCGAATTCTTCTTCTTTTTCTTCTTCTAATTAATCTCAGAGAAGTGAATCCAAGAAAGAAAGATGGGATTGGGGGAAAATATGTTGGTGTTTGTTTTGATAGTGGCGTTGTTGAAAGTAACAGAAGGGATCAGATTTGTGATAGATAGAGATGAGTGTTTCTCACATGAGGTTAAGTACGAGGGTGACACTGTTCATGTTTCTTTCGTTGTTATTAAGGCTGATTCTCCTTGGCATTATGGTGATGAAGGTGTTGATCTTGTGGTAAAAGGACCTGTTGGTGATCAAATTCAAGATTTCCGCGACAAGACCAGTGAAAAGTTTGAGTTTGTGGCTCACAAATCAGGGGTGCATAAGTTCTGTTTCACTAACAAATCTCCTTATCATGAGACTATCGATTTTGATGTGCACGTTGGCCACTTCTCTTACTTCGAGCAACACGCAAAAGACGAGCATTTCAACCCTTTGTTAGAGCAAATAGCGAAGTTGGAAGAAGCTCTTTACAATATTCAGTTCGAACAACATTGGTTAGAAGCGCAGACTGATCGCCAAGCAATAGTGAACGATGGGATGAGCCGAAGAGCAGTACACAAGGCAATCTTTGAATCGGCGGCACTGATTGGAGCTAGTGCATTGCAAGTCTTTCTTCTCCGACGTTTATTTGAGCGAAAGTTGGGAAACTCTAGAGTTTAGAGTTAGAACATGTTCTGAGAATCTGTAACATTATGAATCCCAATGTTATTTTAAACTTTTTGTTTATTCACTGTCATGACATTCATATTTATACTTCTGGTTTACTTTACTCACAATATATTTCTGGAAAAATGCTATTTATTGTTAATTACAGTTTATTTCCGAATTGG
TARGET=118,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2940
PRIMER_LEFT_PENALTY=0.254773
PRIMER_RIGHT_PENALTY=0.039178
PRIMER_LEFT_SEQUENCE=ACTCTCCGTTCACCTCTTCATC
PRIMER_RIGHT_SEQUENCE=TCAACAACGCCACTATCAAAAC
PRIMER_LEFT=22,22
PRIMER_RIGHT=179,22
PRIMER_LEFT_TM=59.745
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=0.3202
PRIMER_LEFT_1_PENALTY=0.281044
PRIMER_RIGHT_1_PENALTY=0.039178
PRIMER_LEFT_1_SEQUENCE=AAACTCTCCGTTCACCTCTTCA
PRIMER_RIGHT_1_SEQUENCE=TCAACAACGCCACTATCAAAAC
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=179,22
PRIMER_LEFT_1_TM=60.281
PRIMER_RIGHT_1_TM=60.039
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.3503
PRIMER_LEFT_2_PENALTY=0.254773
PRIMER_RIGHT_2_PENALTY=0.095538
PRIMER_LEFT_2_SEQUENCE=ACTCTCCGTTCACCTCTTCATC
PRIMER_RIGHT_2_SEQUENCE=GCCACTATCAAAACAAACACCA
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=171,22
PRIMER_LEFT_2_TM=59.745
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=0.3766
PRIMER_LEFT_3_PENALTY=0.281044
PRIMER_RIGHT_3_PENALTY=0.095538
PRIMER_LEFT_3_SEQUENCE=AAACTCTCCGTTCACCTCTTCA
PRIMER_RIGHT_3_SEQUENCE=GCCACTATCAAAACAAACACCA
PRIMER_LEFT_3=20,22
PRIMER_RIGHT_3=171,22
PRIMER_LEFT_3_TM=60.281
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=0.4203
PRIMER_LEFT_4_PENALTY=0.254773
PRIMER_RIGHT_4_PENALTY=0.165541
PRIMER_LEFT_4_SEQUENCE=ACTCTCCGTTCACCTCTTCATC
PRIMER_RIGHT_4_SEQUENCE=CTTTCAACAACGCCACTATCAA
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=182,22
PRIMER_LEFT_4_TM=59.745
PRIMER_RIGHT_4_TM=60.166
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_03778 Ps_contig_mira-and-tgicl-ass_franssen_30999
SEQUENCE=AGAGACCAAACAATACTCTATTTCTTTGGGTTGCAATAAACAAAAAAAAGAAAGAGACCAAACAGGGAGGTCTCTCTCACTCACCTCACATTCTCTTCCTTTACTCTCTCCTTCAATCTGCAAAACACCACCAACAAAACCAATCTCCGCCGGCAAACTTTCCACGCCCTTCCTTTTCCTCACCACGACCTCTGTGACGTCACCCACTAACCTCTCACCACCGTTACCTTGTTCTTCTTCCTTAAAATCTCAAACACATCCACAACCGCCGCCGGTAGCCACCACCACATGACCTTCATGAAAACCCCTTAAACCTTCGTTTCCGCCTCCAAATCACCCAAACTCTTCATCTTCATACCGGATCTACCGCTTCCATCAACATTCCGACCAACGCCGCAAACCGCATCGGAAGCATCATCCCTCACTCACTGCAAACCGCACCGGAGACCCTTGCTCGTAACTCGGCCGTCATGAGACTCACCTCTCGCACATCATCCAAAATGCATCGTTCTGTTTCGCATCTGTAACGGTGATGGCAATAGCGACACGGCTACCCACAGCCGAAAATAACCAGCATCTTCCATTTCCTCCCGCATCTTCCATTTCCTCTGACGTCATGTGGTATCTGTTGCAAGACATACGTCACGTATCGTTTCAAGCATCACAAAGTAACCGCAACGCCGTTCACTTTCGGTAGCTGCTTCTCCACCGAAAGCCATTTGAAAATGAGGTATAACAATCTTAATCTTGAACTTGGTTTTGTTACTGTAAAGAATTGGAGAACACTTTTTTTAAGTATTTCTTTTCTTTCATGTAGAATTTAGGATGAGATTGTTGACACATTGTTGGATAAGTGATAGTTGAGTTTTACATGTTTGCTTTAAAGTAATTCTTTTTCTTACTGATGAAAACAAACCATAGTAGGCTTTAGTTTTCTATTTCTAGTYTTTTTTTTTCTCAGCTTAAAAACGGAAAAGATAAATTATTTAGATATGATAGTTTTCTTTCAATTAGAATTATTTATCCTTAAAATATTAAATTATTCTTAACTTATAATAACAAATGGGTTCTTGCCCATTTTCTTTGTTAATGGTAGCATACTGACATGGCTTTAAACTTTTTTAGATTAGTGGTTCTTAATTAAATTATCAAATAAATTCCAATTTAATAGTACTGCTTGATATGTATATATATATTTATTTATTTTCATCTTTAGGAATCACATCTGTAAGATTTAGTGTGTTTTGATTATAGTGTTTCTATTGGATCATGTTGATGTTGTGATTATCTCAATTACATTATTTGCGACAAATTTGTTTAGGCATATGTGCTAGAGTTTTTGTTTAAGATTTGTTGACATTCAATTTTAATGTAATCCATAAATGACAGAATGTGAGGTTGTTAGAGTCTATTCCATTGCATTGTGTTGTGTGAGTTAAGCTTAATCAAGTGGTTTCAACACCGATTTTATGTTGAAATGTCGTCAAATTTCAAATATATCAATAGTATTTTTCACCGCGTTTGAATTT
TARGET=1186,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03779 Ps_contig_mira-and-tgicl-ass_franssen_30999
SEQUENCE=AGAGACCAAACAATACTCTATTTCTTTGGGTTGCAATAAACAAAAAAAAGAAAGAGACCAAACAGGGAGGTCTCTCTCACTCACCTCACATTCTCTTCCTTTACTCTCTCCTTCAATCTGCAAAACACCACCAACAAAACCAATCTCCGCCGGCAAACTTTCCACGCCCTTCCTTTTCCTCACCACGACCTCTGTGACGTCACCCACTAACCTCTCACCACCGTTACCTTGTTCTTCTTCCTTAAAATCTCAAACACATCCACAACCGCCGCCGGTAGCCACCACCACATGACCTTCATGAAAACCCCTTAAACCTTCGTTTCCGCCTCCAAATCACCCAAACTCTTCATCTTCATACCGGATCTACCGCTTCCATCAACATTCCGACCAACGCCGCAAACCGCATCGGAAGCATCATCCCTCACTCACTGCAAACCGCACCGGAGACCCTTGCTCGTAACTCGGCCGTCATGAGACTCACCTCTCGCACATCATCCAAAATGCATCGTTCTGTTTCGCATCTGTAACGGTGATGGCAATAGCGACACGGCTACCCACAGCCGAAAATAACCAGCATCTTCCATTTCCTCCCGCATCTTCCATTTCCTCTGACGTCATGTGGTATCTGTTGCAAGACATACGTCACGTATCGTTTCAAGCATCACAAAGTAACCGCAACGCCGTTCACTTTCGGTAGCTGCTTCTCCACCGAAAGCCATTTGAAAATGAGGTATAACAATCTTAATCTTGAACTTGGTTTTGTTACTGTAAAGAATTGGAGAACACTTTTTTTAAGTATTTCTTTTCTTTCATGTAGAATTTAGGATGAGATTGTTGACACATTGTTGGATAAGTGATAGTTGAGTTTTACATGTTTGCTTTAAAGTAATTCTTTTTCTTACTGATGAAAACAAACCATAGTAGGCTTTAGTTTTCTATTTCTAGTYTTTTTTTTTCTCAGCTTAAAAACGGAAAAGATAAATTATTTAGATATGATAGTTTTCTTTCAATTAGAATTATTTATCCTTAAAATATTAAATTATTCTTAACTTATAATAACAAATGGGTTCTTGCCCATTTTCTTTGTTAATGGTAGCATACTGACATGGCTTTAAACTTTTTTAGATTAGTGGTTCTTAATTAAATTATCAAATAAATTCCAATTTAATAGTACTGCTTGATATGTATATATATATTTATTTATTTTCATCTTTAGGAATCACATCTGTAAGATTTAGTGTGTTTTGATTATAGTGTTTCTATTGGATCATGTTGATGTTGTGATTATCTCAATTACATTATTTGCGACAAATTTGTTTAGGCATATGTGCTAGAGTTTTTGTTTAAGATTTGTTGACATTCAATTTTAATGTAATCCATAAATGACAGAATGTGAGGTTGTTAGAGTCTATTCCATTGCATTGTGTTGTGTGAGTTAAGCTTAATCAAGTGGTTTCAACACCGATTTTATGTTGAAATGTCGTCAAATTTCAAATATATCAATAGTATTTTTCACCGCGTTTGAATTT
TARGET=1194,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03780 Ps_contig_mira-and-tgicl-ass_franssen_31004
SEQUENCE=AACATTATGAGAACATATGGATAAGATGGATGAGTTTTGTTTTAGTGGTGGAAATGCATTAGATAGATAGATGAATAGTTCTTAGTCTTGGTCATCTTTGGATGGCATCTTGTATATCAAACTCTCTATCACTCCAAGGGAGGAAGAGAAGCCCAAATTGCTTATTTGGTTGGAACATAGGCAGAAAGAGAGGTGGTAATGATGACAAACCTCAGATTAAATACCATGATATTGATCTCACTTTCTCAACTTCTCTGGTTTCCAAAACATTTTTGAAAGGTAAGGAACTAAAATGCTGCTATAGGGCAACAATTGATGGATTTAGTGCAACAAGTTTCCACCAATGTTGTGACTTTAAAGGACCATGTGTAATAATAGGCTACACAAACAACTCTTTCAAGTTTGGTGCATTTAACCCTGAAGGATATAGAAGCACAGATGATTACTATGACACATTTGATGCATTTCTATTTTATTGGATAGAAAATAAAAACGAACCCATCATTTTGCCTAAGGTTGGTGGAAGTGGTGCAGCTTTGTTTGATTATGCTCGCGGCGGGCCACAGTTCGGGGCTGACGGGCTTCTCATCGGGCCCCCGTTAGCACCGGTTATGGGTGGTTTTGCTGGGCCTGACACTAATTCTGGTGTTGGTGATTTAACACAAGCTAAGTCTAGATTGGGATTGTCTTATGCTAAAAGGGAAGATGGCAAGGAGTCTATCTTTGGTGATGAGTCAAGAGTAACTATTCAAGAAGTTGAAGTGTTTTGTAGCCCTAAAATTGCAAGCTTATATTAGAATTTCATGAAAAATACTATACTATACTAATTCTCCTACTTTGAAGCTGTTGAAAATCGAGTATTTGAAAAATTGTTTTTCTTTTATCAGAGAGATTGTACTTTAAAACTAGTACTGCTTATTTATCATTCTAAGTTATAATGCCACTCCGAGTAAGATTTCAGCTG
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.1013
PRIMER_LEFT_PENALTY=2.970986
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=GGATGAGTTTTGTTTTAGTGGTGG
PRIMER_RIGHT_SEQUENCE=CATTACCACCTCTCTTTCTGCC
PRIMER_LEFT=27,24
PRIMER_RIGHT=200,22
PRIMER_LEFT_TM=60.971
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=3.1071
PRIMER_LEFT_1_PENALTY=2.970986
PRIMER_RIGHT_1_PENALTY=0.136083
PRIMER_LEFT_1_SEQUENCE=GGATGAGTTTTGTTTTAGTGGTGG
PRIMER_RIGHT_1_SEQUENCE=TAAGCAATTTGGGCTTCTCTTC
PRIMER_LEFT_1=27,24
PRIMER_RIGHT_1=163,22
PRIMER_LEFT_1_TM=60.971
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=3.1751
PRIMER_LEFT_2_PENALTY=2.970986
PRIMER_RIGHT_2_PENALTY=0.204119
PRIMER_LEFT_2_SEQUENCE=GGATGAGTTTTGTTTTAGTGGTGG
PRIMER_RIGHT_2_SEQUENCE=TCCTCCCTTGGAGTGATAGAGA
PRIMER_LEFT_2=27,24
PRIMER_RIGHT_2=143,22
PRIMER_LEFT_2_TM=60.971
PRIMER_RIGHT_2_TM=60.204
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=3.1980
PRIMER_LEFT_3_PENALTY=2.970986
PRIMER_RIGHT_3_PENALTY=0.226971
PRIMER_LEFT_3_SEQUENCE=GGATGAGTTTTGTTTTAGTGGTGG
PRIMER_RIGHT_3_SEQUENCE=CCACCTCTCTTTCTGCCTATGT
PRIMER_LEFT_3=27,24
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=60.971
PRIMER_RIGHT_3_TM=59.773
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=3.1980
PRIMER_LEFT_4_PENALTY=2.970986
PRIMER_RIGHT_4_PENALTY=0.226971
PRIMER_LEFT_4_SEQUENCE=GGATGAGTTTTGTTTTAGTGGTGG
PRIMER_RIGHT_4_SEQUENCE=ACCACCTCTCTTTCTGCCTATG
PRIMER_LEFT_4=27,24
PRIMER_RIGHT_4=196,22
PRIMER_LEFT_4_TM=60.971
PRIMER_RIGHT_4_TM=59.773
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_03781 Ps_contig_mira-and-tgicl-ass_franssen_31004
SEQUENCE=AACATTATGAGAACATATGGATAAGATGGATGAGTTTTGTTTTAGTGGTGGAAATGCATTAGATAGATAGATGAATAGTTCTTAGTCTTGGTCATCTTTGGATGGCATCTTGTATATCAAACTCTCTATCACTCCAAGGGAGGAAGAGAAGCCCAAATTGCTTATTTGGTTGGAACATAGGCAGAAAGAGAGGTGGTAATGATGACAAACCTCAGATTAAATACCATGATATTGATCTCACTTTCTCAACTTCTCTGGTTTCCAAAACATTTTTGAAAGGTAAGGAACTAAAATGCTGCTATAGGGCAACAATTGATGGATTTAGTGCAACAAGTTTCCACCAATGTTGTGACTTTAAAGGACCATGTGTAATAATAGGCTACACAAACAACTCTTTCAAGTTTGGTGCATTTAACCCTGAAGGATATAGAAGCACAGATGATTACTATGACACATTTGATGCATTTCTATTTTATTGGATAGAAAATAAAAACGAACCCATCATTTTGCCTAAGGTTGGTGGAAGTGGTGCAGCTTTGTTTGATTATGCTCGCGGCGGGCCACAGTTCGGGGCTGACGGGCTTCTCATCGGGCCCCCGTTAGCACCGGTTATGGGTGGTTTTGCTGGGCCTGACACTAATTCTGGTGTTGGTGATTTAACACAAGCTAAGTCTAGATTGGGATTGTCTTATGCTAAAAGGGAAGATGGCAAGGAGTCTATCTTTGGTGATGAGTCAAGAGTAACTATTCAAGAAGTTGAAGTGTTTTGTAGCCCTAAAATTGCAAGCTTATATTAGAATTTCATGAAAAATACTATACTATACTAATTCTCCTACTTTGAAGCTGTTGAAAATCGAGTATTTGAAAAATTGTTTTTCTTTTATCAGAGAGATTGTACTTTAAAACTAGTACTGCTTATTTATCATTCTAAGTTATAATGCCACTCCGAGTAAGATTTCAGCTG
TARGET=811,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3924
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.261998
PRIMER_LEFT_SEQUENCE=TGGCAAGGAGTCTATCTTTGGT
PRIMER_RIGHT_SEQUENCE=CTGAAATCTTACTCGGAGTGGC
PRIMER_LEFT=706,22
PRIMER_RIGHT=960,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.262
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=255
PRIMER_PAIR_PENALTY_1=0.3924
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.261998
PRIMER_LEFT_1_SEQUENCE=GGCAAGGAGTCTATCTTTGGTG
PRIMER_RIGHT_1_SEQUENCE=CTGAAATCTTACTCGGAGTGGC
PRIMER_LEFT_1=707,22
PRIMER_RIGHT_1=960,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.262
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=254
PRIMER_PAIR_PENALTY_2=0.3924
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.261998
PRIMER_LEFT_2_SEQUENCE=GGCAAGGAGTCTATCTTTGGTG
PRIMER_RIGHT_2_SEQUENCE=GCTGAAATCTTACTCGGAGTGG
PRIMER_LEFT_2=707,22
PRIMER_RIGHT_2=961,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=60.262
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=0.3924
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.261998
PRIMER_LEFT_3_SEQUENCE=TGGCAAGGAGTCTATCTTTGGT
PRIMER_RIGHT_3_SEQUENCE=GCTGAAATCTTACTCGGAGTGG
PRIMER_LEFT_3=706,22
PRIMER_RIGHT_3=961,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=60.262
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.6471
PRIMER_LEFT_4_PENALTY=0.385098
PRIMER_RIGHT_4_PENALTY=0.261998
PRIMER_LEFT_4_SEQUENCE=ATGGCAAGGAGTCTATCTTTGG
PRIMER_RIGHT_4_SEQUENCE=CTGAAATCTTACTCGGAGTGGC
PRIMER_LEFT_4=705,22
PRIMER_RIGHT_4=960,22
PRIMER_LEFT_4_TM=59.615
PRIMER_RIGHT_4_TM=60.262
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_03782 Ps_contig_mira-and-tgicl-ass_franssen_31007
SEQUENCE=AGGGAAAACTTAAACAACAAGTCAAAATATAGAGTATCGATTTCTTTTTTTNAATTAATTTTTTTCTAGACGCGGAAATTCTTTCCTGTGAAGGTTTGTCCGAACTGCCAATGAGGTGGTGCCACGTTCCATGACGTTGATGTACGACGGTCACTGCCGGTGACCCTAAAAGAGAGAGCCTGGCCCACAAAAACTGCGTTGGATTGCCAGTTCTGCCCCCAGTTACGGCTCATTGTCATCCACGCTGTGTTGGTTCCTTTTACACTCACGCGCACGATATCCCCTGCACCCGCGACGTTGGTGATTAGAACCAAGTTGAAGTAACGGAAACCGTTGATTGTGAATCTGATGCCTCCTGCCTTTCTGCATGGCACACGGCGGTAAGCAACGGGGACGATTCCGGCGCGGTATTGAGCGATCTTAAGGAACATGGGCATGGCAAGATCAAAGTGTGGTCGAGGTGGGTTACACCAGCCACCATTGTCACTAGGTTCAGCGAAATTTGGAGGACAAAAGTTTGTTGCAGTGATTAAGATGGAAGGGTTTCCAGGGTTACACCACCGTGGATCTTGGTCACACTTGAGCTCAAAACATGCACCGCAACTTAAGCCATTGTTGAATAGTGCTGTGCTTAGTGCTGCGGTGTTGACGCCGTAGCCTTGGCTGTATAAGTTTCCGTACCCACAAGCACCACCCATTGTTCCGGAGGCATCACTGCCGCCGTAGAAAGTAGCATGAGCACTGGTCCAAGGGCCGCCGGTGTAAACTCCAGGGATTCGAGCGGTGGTTGGAGCAAAAAGTGAGGTTAGTGATGCAATGGTGAGGAGAAGAATTGCAGCCATTGCAAGAGAGAGAGAGAGAGAAAGAGAGGTGAGTGATTTGGGGATGAGTCCCCCGNTTTTGAGGGTGTTGA
TARGET=847,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7579
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.755547
PRIMER_LEFT_SEQUENCE=GTGTAAACTCCAGGGATTCGAG
PRIMER_RIGHT_SEQUENCE=GACTCATCCCCAAATCACTCAC
PRIMER_LEFT=759,22
PRIMER_RIGHT=891,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.756
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=1.0067
PRIMER_LEFT_1_PENALTY=0.251135
PRIMER_RIGHT_1_PENALTY=0.755547
PRIMER_LEFT_1_SEQUENCE=ATAGTGCTGTGCTTAGTGCTGC
PRIMER_RIGHT_1_SEQUENCE=GACTCATCCCCAAATCACTCAC
PRIMER_LEFT_1=619,22
PRIMER_RIGHT_1=891,22
PRIMER_LEFT_1_TM=59.749
PRIMER_RIGHT_1_TM=60.756
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=1.0475
PRIMER_LEFT_2_PENALTY=0.291945
PRIMER_RIGHT_2_PENALTY=0.755547
PRIMER_LEFT_2_SEQUENCE=CCGTAGCCTTGGCTGTATAAGT
PRIMER_RIGHT_2_SEQUENCE=GACTCATCCCCAAATCACTCAC
PRIMER_LEFT_2=651,22
PRIMER_RIGHT_2=891,22
PRIMER_LEFT_2_TM=59.708
PRIMER_RIGHT_2_TM=60.756
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=1.0839
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=1.081589
PRIMER_LEFT_3_SEQUENCE=GTGTAAACTCCAGGGATTCGAG
PRIMER_RIGHT_3_SEQUENCE=GACTCATCCCCAAATCACTCA
PRIMER_LEFT_3=759,22
PRIMER_RIGHT_3=891,21
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=59.918
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=1.1932
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=1.190908
PRIMER_LEFT_4_SEQUENCE=GTGTAAACTCCAGGGATTCGAG
PRIMER_RIGHT_4_SEQUENCE=ATCCCCAAATCACTCACCTCT
PRIMER_LEFT_4=759,22
PRIMER_RIGHT_4=886,21
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=59.809
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_03783 Ps_contig_mira-and-tgicl-ass_franssen_31010
SEQUENCE=AGGAGATCTGGGAAACCAAAAGCAAGCAAGCTTTGGGTGATGCTAAGCTAATCTCACCTTTTTTATCTCTAATCTCAAATAAAACCCTAATTAATGACTAATTAATTACCTTTTGATTATCATATACTACTAGGGTTTGTATATATGAAAGCGAAGCAACAATCTATGAACAACGATGATGAAGGGGATGGAGTCTGAACTGAAAGACTTGAATTCCAAAGCAGCGAAATCGAATGGAAATGACACCGTTCGCGACGATCGTCCTCTTCTCAGATCAGATTCAGTATCTTCCACCACCACCTCAACCACCGCAACCGCAATCACCGCGGCGGAGCTACAAGAGCTTGAGAAGAAATTCGCGGCGTATGTTCGTCGCGATGTTTATGGAACCATGGGGAGAGGAGAGTTAGCAGCTAAGGAGAAACTATTGCTTGGTTTCGCACTTGTTACGCTTCTTCCAATTAGAGTGATTCTTGCTGGGATTGTTTTGGCAGTTTATTACGTTATCTGTAGGGTTTGTACATTGTTTTTGAGTCCGAATCGTGAAGATGAACAAGAGGATTATGCTCATATGGGAGGGTGGAGAAGGATTGTTATTGTTCGGTGTGGAAAAGCTCTTTCCAGGGTTATGCTTTTCGTTTTTGGATTTTATTGGATTCCTGATTCTACTACCTCAATCAATCAGGAAGACAAACCTCAGCCTGAAAAGACGGAAAGGCCTGGTGTAATAATATCTAATCATGTATCTTACTTGGATATTTTGTATCACATGTCTTCCTCTTTCCCAAGTTTTGTTGCTAAGAGATCGGTGGCCAAGCTTCCTCTTGTTGGTCTCATCAGCAAGTGCCTTGGTTGCATCTATGTTCAGCGGGAATCAAAGTCATCTGACTTCAAGGGTGTTTCAGCTGTTGTCACGGAAAGAATTCGAGAAGCACATCAGAATGAATCTGCTCCATTAATGATGTTATTTCCAGAAGGTACAACCACAAATGGAGATTTCCTCCTTCCATTCAAGACTGGTGGTTTTTTGGCAAAGGCACCAGTACTTCCTGTAATTTTAAAATACCATTACCAAAGATTTAGCCCTGCCTGGGATTCAATTTCTGGGGTGCGCCATGTGATATTTCTCCTATGTCAGTTTGTAAACTATATGGAGGCGACACAATTACCTCTTTACTATCCCTCACAACAGGAAAAGGATGATCCCAAATTGTATGCGGATAATGTTAGAAGGTTGATGGCTAGTGAGGGTAATTTGACACTTTCTGATATTGGGCTAGCTGAAAAACGAATTTATCACGCTGCTCTCAATGGTAATAATAGCCTGCCTAGTGTTTTGCATCAGAAAGACGATTGATAATTTCATGAACCCGGCCTCAATTGATATGTAGTTCCATTCGAGTTTTGGTTTCAAACTTAGAATTTGCTTTATATATGAATGGACAGCTTGTGTGAGGTGGGGGTGCTATAATCGCATAAACTACTGAATAGTACTTGTGTAATTTTCTTGTAAATACTGTAACCAATAATGTATTTTAATTGTTGGTGAATGAGAGACGCAATTCAAGAGACCAAAAGTTTTATTGTGATAAATGACCTAACTCATTTGAGAAAAGCCATGTAAAGCTTAGACTGAATCTTCTGTTTCGTGTTCCTGCAGGTTTGTTTTCCCAAGGATAGTTCTTCGGATATTCGCATGTATATTCGTTGTATGGGTGTATTATTTGTCAAGGGAAAAAGAAAAAAGGAACTAAAT
TARGET=20,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.5424
PRIMER_LEFT_PENALTY=3.432264
PRIMER_RIGHT_PENALTY=0.110131
PRIMER_LEFT_SEQUENCE=AGGAGATCTGGGAAACCAAA
PRIMER_RIGHT_SEQUENCE=CTTTCAGTTCAGACTCCATCCC
PRIMER_LEFT=0,20
PRIMER_RIGHT=205,22
PRIMER_LEFT_TM=58.568
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=3.6093
PRIMER_LEFT_1_PENALTY=3.432264
PRIMER_RIGHT_1_PENALTY=0.177008
PRIMER_LEFT_1_SEQUENCE=AGGAGATCTGGGAAACCAAA
PRIMER_RIGHT_1_SEQUENCE=CCCTTCATCATCGTTGTTCATA
PRIMER_LEFT_1=0,20
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=58.568
PRIMER_RIGHT_1_TM=59.823
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=3.7043
PRIMER_LEFT_2_PENALTY=3.432264
PRIMER_RIGHT_2_PENALTY=0.271999
PRIMER_LEFT_2_SEQUENCE=AGGAGATCTGGGAAACCAAA
PRIMER_RIGHT_2_SEQUENCE=TGTTCATAGATTGTTGCTTCGC
PRIMER_LEFT_2=0,20
PRIMER_RIGHT_2=171,22
PRIMER_LEFT_2_TM=58.568
PRIMER_RIGHT_2_TM=60.272
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=4.6272
PRIMER_LEFT_3_PENALTY=3.432264
PRIMER_RIGHT_3_PENALTY=1.194956
PRIMER_LEFT_3_SEQUENCE=AGGAGATCTGGGAAACCAAA
PRIMER_RIGHT_3_SEQUENCE=CCCTTCATCATCGTTGTTCAT
PRIMER_LEFT_3=0,20
PRIMER_RIGHT_3=185,21
PRIMER_LEFT_3_TM=58.568
PRIMER_RIGHT_3_TM=59.805
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=4.6628
PRIMER_LEFT_4_PENALTY=3.432264
PRIMER_RIGHT_4_PENALTY=1.230523
PRIMER_LEFT_4_SEQUENCE=AGGAGATCTGGGAAACCAAA
PRIMER_RIGHT_4_SEQUENCE=AGACTCCATCCCCTTCATCAT
PRIMER_LEFT_4=0,20
PRIMER_RIGHT_4=195,21
PRIMER_LEFT_4_TM=58.568
PRIMER_RIGHT_4_TM=59.769
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=196
=
PRIMER_SEQUENCE_ID=SSR_03784 Ps_contig_mira-and-tgicl-ass_franssen_31014
SEQUENCE=GGAAATCTCACTGTTGYGAAACCTTATGCCTTTTATTATAGTTTTTATATAGTTATAATAATTATAATTATAACATATAATGATAGATTATAACAGACTGTTACAATTTCATACATTCACTATTTCAAAACTAAGCCAAGAGCTATTAGTGTAGAAGATACAACTAAATGAAGTAATTTACATACAATTCTAAATAGATTGAATGAACTCCATTAATTAATTACATTAACTTACAAATATGGAACACTAATAGTACACAAATGAGGAGCACATTCACAATAGGCCAGCAAAGTAGTGAATGAGTTGTGCAAAATCGATCTAAATTACCCATAGGGAAGAGTCAGAGATGCTTGCCTATGCTTCAAGAAAATAAAGACTCTAAAGTTAGTTCTCTAAACTTTGGTCAGCATCAGGTTGATTTGAAGTAATAGTCCCACCATTCATTCTAGCACGCGCCTCTGCAAACATCCTTTGCTGCTCTGCTAATGCCTGTTCCTCTGTCATCTCAGCTGCGCCACTCCACTTGGCACCTTTTATAGAGTCCTGCACTGTCTCCATTTTGTGCTGTTCATATGCTGCATAAACCTCCTCAATGTACTCACCAAATCCAAGAACCCCTAAAGCCTTCAACACGTGCTCAGGTGCAATCGTCCTTCTTTCCTCTCTGTTACATACTTCATTTGACTCTGATGAGATAAGGTTTATGAACTCTACACAGCATTCAATCAACAAATCCTGTGTATCTCTTGCAACTCGAACATCGGGTGGTAACATCTCTTTTATAATTTTCGTCATCGTGGCTTTAGGAAGCGACGCATCTTCCTTTGATTTTGCAACTATGTCCATGGGTTCCATGTTTTTTGGCTGATCGATTGAAAAAATAGATGAGTAAAAGGAGGAAGATGAAATCAACTGATTGAAGATCGGGGATGAAGGGACATGGCTGATGATTTAATATATGAAAAAAGAAAAGAAGAAGAAAAGGGTTGGTGGTTTGAAGTCTGGATGGATAAGAACGAAAGAGAAGAGGAAATGTTTGTGCTTCCGTCATCGCCCTCCCG
TARGET=212,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03785 Ps_contig_mira-and-tgicl-ass_franssen_31017
SEQUENCE=CGGGGTCGTGGGGCGTAACTCTCTCGGTAACGAAAATTTCTCAGAGAAAGGAACGAGTGATAAGAGTGAAGGTGATCAAAGATGGCATTAGTTTCTGGAGGAAGATCAACCCTAAACCCGAATGCGCCTCTTTACATTCCGGCTGCTTTTCGTCAAGTGGAGGATTTTTCTCCGGAATGGTGGCAGCTGGTGACGACACTGACATGGTACCATGACTACTGGCTTAACCAGCAGCATGATGATGAAGGCTTCTACGACGATGAATTTGATGGCAATGATGTGGTTGATTTGCTTCCTGATGCATTTGATCTTGACGTGAATGATGATGATGATCTTTCTATTGAAGCTCAGCTTGAAGAGTTTATCAAGTCTTCTGAAGCTCAAGATGAAGCGTTTGCAGAGTCCGGATTGAAGAAATTTACTGGATGAGGCGAAGATGGAACTTCCAACATATCTTCTACCAAAGAGGCTGATTGCAATGCTTTCGGTCATTTAGATAAATGTACCATGTCAGTTTTAACTTTCTTTAACTTTTGTATAGGCAAGGTTTCGATGTTCCTTCTTAGTTACCCAAATCTATGGTAAATAGAGAGAAAAAATGTAAAAAAATATTTCTAGTATTGTTGCAACTGCTGCTATAAGTAATATGAAAAAAAAAAGAAAAAAAATNCAAAAGAGAAGTCGGGATTTGTTCAATTAAC
TARGET=320,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4496
PRIMER_LEFT_PENALTY=0.080962
PRIMER_RIGHT_PENALTY=0.368624
PRIMER_LEFT_SEQUENCE=TCCTGATGCATTTGATCTTGAC
PRIMER_RIGHT_SEQUENCE=AAGAAGGAACATCGAAACCTTG
PRIMER_LEFT=293,22
PRIMER_RIGHT=563,22
PRIMER_LEFT_TM=60.081
PRIMER_RIGHT_TM=59.631
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.6610
PRIMER_LEFT_1_PENALTY=0.292414
PRIMER_RIGHT_1_PENALTY=0.368624
PRIMER_LEFT_1_SEQUENCE=CTTCCTGATGCATTTGATCTTG
PRIMER_RIGHT_1_SEQUENCE=AAGAAGGAACATCGAAACCTTG
PRIMER_LEFT_1=291,22
PRIMER_RIGHT_1=563,22
PRIMER_LEFT_1_TM=59.708
PRIMER_RIGHT_1_TM=59.631
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.8215
PRIMER_LEFT_2_PENALTY=0.080962
PRIMER_RIGHT_2_PENALTY=0.740565
PRIMER_LEFT_2_SEQUENCE=TCCTGATGCATTTGATCTTGAC
PRIMER_RIGHT_2_SEQUENCE=GCAATCAGCCTCTTTGGTAGAA
PRIMER_LEFT_2=293,22
PRIMER_RIGHT_2=476,22
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.741
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=0.9592
PRIMER_LEFT_3_PENALTY=0.590581
PRIMER_RIGHT_3_PENALTY=0.368624
PRIMER_LEFT_3_SEQUENCE=GCTTCCTGATGCATTTGATCTT
PRIMER_RIGHT_3_SEQUENCE=AAGAAGGAACATCGAAACCTTG
PRIMER_LEFT_3=290,22
PRIMER_RIGHT_3=563,22
PRIMER_LEFT_3_TM=60.591
PRIMER_RIGHT_3_TM=59.631
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.9639
PRIMER_LEFT_4_PENALTY=0.080962
PRIMER_RIGHT_4_PENALTY=0.882980
PRIMER_LEFT_4_SEQUENCE=TCCTGATGCATTTGATCTTGAC
PRIMER_RIGHT_4_SEQUENCE=ATCTTCGCCTCATCCAGTAAAT
PRIMER_LEFT_4=293,22
PRIMER_RIGHT_4=437,22
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=59.117
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_03786 Ps_contig_mira-and-tgicl-ass_franssen_31018
SEQUENCE=AAAAGATAGAAATTGTGATNATTATCATCATCAGAAGAAAAAGATACTATGTGGTGAAACCGAGGGCCAAACTCCAACAACAACAAAAATGGCGCTTCAAACGCAATTCGCAACTTATTTCACCACCAAAATCTATCATCATTCGTTCTCTCCCATCCTCACAGTTAGATCTGAAAAGCAATCTGAACCGTCCGATTCAATTCCCAAAAAATCCGGGGTGGGTTTTGGTCCGTCTAATGTGAACACTAAAAGGAAGCAAAACACTAAAAGGAAGCAGAAGGGACAAAGAGAAAGAGCTTCGATAATTCGTCGTAATCCAGTTGAAAAACCCGCTTTGGTTTCGGAACCAGAACCGGTTCAACAGGAACAGGGTGCTTATGAAAACGCCTTTATTCTTGCATGGCTTGGTTTTGGTTCTGTTATTCTTGTTGAGGGTCTTGCTCTTGCAGCTTCAGGTTTCTTTCCTGAAAAGTGGGACAAGATCTTTGTCAAGTATCTTTATCCATCCTTTACCCCTACAGTTTTCTTGTTTGTTGCTGGAGCAATAGCATTTGGAGTGGTTAAATATAAGCAGAATGAGAAACTTACAGAGCGGAAATAGCGTGTTTGGTGTAGTAATGTTGAAACCTTTGATGCATCGATGATTGTAATCTTATTATGGCTTCAATTTATCAAAATCGAAGATGACAAAGGTAACAGGAATGACGTGTGAACACAAGAAGACTCATTGTGAGCAATTTCTGTCGATACTAAGCAACTTCTTGAATGCCATCATATGGCCAAATACCTGAGGTTTTTGAAATGGATTCAATCCTTTTTGTAATTAATTTGATCACTTCTTTTCTAGCTATGAAACTTCTTTTGATATATATATTATGTACTGAGCTGCATTTAATCCCTTTACTCTTAAAATTGCTGTGTAAACTACATGTTTGTGTGATGATTTCATGGTTCCAAATCATATGCATATGAGAGATAGGAGARNANAARACTCTAAAGATAACTTTGTAA
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03787 Ps_contig_mira-and-tgicl-ass_franssen_31018
SEQUENCE=AAAAGATAGAAATTGTGATNATTATCATCATCAGAAGAAAAAGATACTATGTGGTGAAACCGAGGGCCAAACTCCAACAACAACAAAAATGGCGCTTCAAACGCAATTCGCAACTTATTTCACCACCAAAATCTATCATCATTCGTTCTCTCCCATCCTCACAGTTAGATCTGAAAAGCAATCTGAACCGTCCGATTCAATTCCCAAAAAATCCGGGGTGGGTTTTGGTCCGTCTAATGTGAACACTAAAAGGAAGCAAAACACTAAAAGGAAGCAGAAGGGACAAAGAGAAAGAGCTTCGATAATTCGTCGTAATCCAGTTGAAAAACCCGCTTTGGTTTCGGAACCAGAACCGGTTCAACAGGAACAGGGTGCTTATGAAAACGCCTTTATTCTTGCATGGCTTGGTTTTGGTTCTGTTATTCTTGTTGAGGGTCTTGCTCTTGCAGCTTCAGGTTTCTTTCCTGAAAAGTGGGACAAGATCTTTGTCAAGTATCTTTATCCATCCTTTACCCCTACAGTTTTCTTGTTTGTTGCTGGAGCAATAGCATTTGGAGTGGTTAAATATAAGCAGAATGAGAAACTTACAGAGCGGAAATAGCGTGTTTGGTGTAGTAATGTTGAAACCTTTGATGCATCGATGATTGTAATCTTATTATGGCTTCAATTTATCAAAATCGAAGATGACAAAGGTAACAGGAATGACGTGTGAACACAAGAAGACTCATTGTGAGCAATTTCTGTCGATACTAAGCAACTTCTTGAATGCCATCATATGGCCAAATACCTGAGGTTTTTGAAATGGATTCAATCCTTTTTGTAATTAATTTGATCACTTCTTTTCTAGCTATGAAACTTCTTTTGATATATATATTATGTACTGAGCTGCATTTAATCCCTTTACTCTTAAAATTGCTGTGTAAACTACATGTTTGTGTGATGATTTCATGGTTCCAAATCATATGCATATGAGAGATAGGAGARNANAARACTCTAAAGATAACTTTGTAA
TARGET=865,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03788 Ps_contig_mira-and-tgicl-ass_franssen_31021
SEQUENCE=TCGGGGGAGTTGGTAGAGTGGTATATCGAACAACATTAGAATTAAATATTGAAAAAAAAAGGAAAACAGTGTAAGGTTGTGAGGTACAGAGGAGATGAAAAGGAAGTGAGAACAGAGAGAGTGAATCTGAAAATAGATAGATAGATCTGAGAAAAAAATGTCACGGGTTAGTAGATCTGATTCAGGGATTTGGTTAAGATGGTGTTTGGTTTTGTTTGCAATGGTATCCGCTTTTGGTGTATGCGGTCCTGCTCTTTACTGGAGATTCAAAAAGGGCATCACTCTTCGCAATTCCAAAACCTCTTGTCCTCCTTGCATCTGTGATTGTCCTCCTCCTCTCTCCCTCTTCCAACTCGCTCCTGGGCTAGCCAATCTCTCTGTCACAGATTGTGGAGGCAATGATCCGGAACTAAAGGAAGAGATGGAGAAGCAATTTGTCGACCTTCTAACTGAGGAGCTAAAGTTACAAGAGTCAGTTTCTGAAGCGCACACGCGGCATATGAACATAACTTTAGCCGAAGCAAAAAGAGTGGGATCTCAATACCAAAGAGAAGCAGACAAATGTGTTGCTGCGACTGAAACGTGCGAGCAGGCACGAGAGCGGGCTGAAGCCAAGCTCACCAAGGAGAGAAAATTGACTTTGATATGGGAGAAACGGGCACGCCAAATAGGCTGGGAAGGAGAATAACTGGATCATGTTTATTATTTTTATAGAAGTTATTTATATGGTTATAATCATCATTTTATATATAAGCATACAAGCAAAATACTCGAGTTGTCAAATTATTCTCCTCTTACTGGGCTTGTTTGTGACTACAAGGTGCTAGAATCAACAATTTTTCCCTCACTGATGATACCAATTCATGATCCCACATGGGAAAAATGTAAAAGAATCTTGCCAGATATACTACTTATGACCTCGTTTAATATATGATACAACAAATTCTGTCGGTTGGGCTGCTNTTGGTTCATTCCCACATTTTCTCTTTCTTTTTCTCAGCATTACAAAATAAAGGGCAAACTGTCTATTTTAGTTAACT
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8686
PRIMER_LEFT_PENALTY=0.477653
PRIMER_RIGHT_PENALTY=0.390906
PRIMER_LEFT_SEQUENCE=GGGGGAGTTGGTAGAGTGGTAT
PRIMER_RIGHT_SEQUENCE=GCCCTTTTTGAATCTCCAGTAA
PRIMER_LEFT=2,22
PRIMER_RIGHT=276,22
PRIMER_LEFT_TM=60.478
PRIMER_RIGHT_TM=59.609
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.8000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.8794
PRIMER_LEFT_1_PENALTY=0.477653
PRIMER_RIGHT_1_PENALTY=0.401769
PRIMER_LEFT_1_SEQUENCE=GGGGGAGTTGGTAGAGTGGTAT
PRIMER_RIGHT_1_SEQUENCE=GAGTGATGCCCTTTTTGAATCT
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=283,22
PRIMER_LEFT_1_TM=60.478
PRIMER_RIGHT_1_TM=59.598
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.8000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=0.8794
PRIMER_LEFT_2_PENALTY=0.477653
PRIMER_RIGHT_2_PENALTY=0.401769
PRIMER_LEFT_2_SEQUENCE=GGGGGAGTTGGTAGAGTGGTAT
PRIMER_RIGHT_2_SEQUENCE=AGTGATGCCCTTTTTGAATCTC
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=282,22
PRIMER_LEFT_2_TM=60.478
PRIMER_RIGHT_2_TM=59.598
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.8000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=281
PRIMER_PAIR_PENALTY_3=0.8794
PRIMER_LEFT_3_PENALTY=0.477653
PRIMER_RIGHT_3_PENALTY=0.401769
PRIMER_LEFT_3_SEQUENCE=GGGGGAGTTGGTAGAGTGGTAT
PRIMER_RIGHT_3_SEQUENCE=AGAGTGATGCCCTTTTTGAATC
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=284,22
PRIMER_LEFT_3_TM=60.478
PRIMER_RIGHT_3_TM=59.598
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.8000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=1.0703
PRIMER_LEFT_4_PENALTY=0.477653
PRIMER_RIGHT_4_PENALTY=0.592680
PRIMER_LEFT_4_SEQUENCE=GGGGGAGTTGGTAGAGTGGTAT
PRIMER_RIGHT_4_SEQUENCE=GAAGAGTGATGCCCTTTTTGAA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=286,22
PRIMER_LEFT_4_TM=60.478
PRIMER_RIGHT_4_TM=60.593
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.8000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_03789 Ps_contig_mira-and-tgicl-ass_franssen_31022
SEQUENCE=TAAGCTAGTTTGGTTATTCAACGCAGAGTACGCGGGGATCACACCATTGAGTGGAGAGTGGTATATCGAACAACATTAGAATTAAATATTAGAAAAAAAAAGGAAAACAGTGTAAGGTTATGAGGTACTGAGGAGATGAAAAGGAAGTGAGAACAGAGAGAGTGAAYCTGAAAATAGATAGATAGATCTAGAAGAAAAAAATGTCACGGGTTAGTAGATCTGATTCGAGGGTATTTTGGGTTAAAGATTGGGTTGTTTTGGTTTTTGTTTGCAATTGGTATCCNGTCTTTTTGG
TARGET=174,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03790 Ps_contig_mira-and-tgicl-ass_franssen_31029
SEQUENCE=AAGACAGTGGTATTCAACGACAGAGTACGCGGGNGCTTCCCATAACTTGCGAACTTCACTTAAACTCTCTGCTCCCTTTTCTATCTTCCACCAATTTTCCTTAACTTCTTCTCCTCCTCCTCCTCCATCGGAATCCATGGCGCACAACAGATTCGTCATTGAAGTCGAAAAAGCCAAGGAAGCGACTGATGCTAGACCTTCTAGAGGACCTGCGTACCGTAGCATCTTCGCTAAAGATGGATTTCCTGCTCCCGTTCAAGGTCTTGATAGTTGCTGGGACGTTTTCCGAATGTCTGTTGAGAAATATCCAAGTAATCCTATGCTTGGTAACCGGGAGATTGTGGATGGAAAGGCTGGCGGTTACAAGTGGCAAACTTACAAAGAAGTATATGATATGGTGATCAAAGTCGGAAATTCCATCCGTAGCTGTGGTTATGGAGAAGGTGTAAAATGTGGCATTTATGGTGCCAATTCTTCAGAGTGGATTATGAGCATGGAGGCTTGCAATGCTCATGGTCTTCTTTGCGTTCCTTTATATGACACCTTAGGTTCTGGGGCCATAGAATTTATTATATCCCATGCAGAAGTTTCAATTGCATTTGCAGAAGAAAAAGAAAATACCCGAGCTGTTGAAGACATTTCCAAGTGCAACCAAATATCTCAGGACAATTGTGAGCTTCGGAAAGGTTACCCCTGAACAAAAGCAAGAAGTTGAAAAGTTTGGATTGGCAATATATTCATGGACTGGTATTTTTACAATTGGGTGAAAGTC
TARGET=111,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0635
PRIMER_LEFT_PENALTY=0.061184
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=TCCCTTTTCTATCTTCCACCAA
PRIMER_RIGHT_SEQUENCE=ACTATCAAGACCTTGAACGGGA
PRIMER_LEFT=72,22
PRIMER_RIGHT=270,22
PRIMER_LEFT_TM=59.939
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.1079
PRIMER_LEFT_1_PENALTY=0.061184
PRIMER_RIGHT_1_PENALTY=0.046672
PRIMER_LEFT_1_SEQUENCE=TCCCTTTTCTATCTTCCACCAA
PRIMER_RIGHT_1_SEQUENCE=AAGGTCTAGCATCAGTCGCTTC
PRIMER_LEFT_1=72,22
PRIMER_RIGHT_1=199,22
PRIMER_LEFT_1_TM=59.939
PRIMER_RIGHT_1_TM=60.047
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=128
PRIMER_PAIR_PENALTY_2=0.1079
PRIMER_LEFT_2_PENALTY=0.061184
PRIMER_RIGHT_2_PENALTY=0.046672
PRIMER_LEFT_2_SEQUENCE=TCCCTTTTCTATCTTCCACCAA
PRIMER_RIGHT_2_SEQUENCE=GTCTAGCATCAGTCGCTTCCTT
PRIMER_LEFT_2=72,22
PRIMER_RIGHT_2=196,22
PRIMER_LEFT_2_TM=59.939
PRIMER_RIGHT_2_TM=60.047
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.1079
PRIMER_LEFT_3_PENALTY=0.061184
PRIMER_RIGHT_3_PENALTY=0.046672
PRIMER_LEFT_3_SEQUENCE=TCCCTTTTCTATCTTCCACCAA
PRIMER_RIGHT_3_SEQUENCE=GAAGGTCTAGCATCAGTCGCTT
PRIMER_LEFT_3=72,22
PRIMER_RIGHT_3=200,22
PRIMER_LEFT_3_TM=59.939
PRIMER_RIGHT_3_TM=60.047
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.1518
PRIMER_LEFT_4_PENALTY=0.149516
PRIMER_RIGHT_4_PENALTY=0.002310
PRIMER_LEFT_4_SEQUENCE=CTGCTCCCTTTTCTATCTTCCA
PRIMER_RIGHT_4_SEQUENCE=ACTATCAAGACCTTGAACGGGA
PRIMER_LEFT_4=68,22
PRIMER_RIGHT_4=270,22
PRIMER_LEFT_4_TM=59.850
PRIMER_RIGHT_4_TM=59.998
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_03791 Ps_contig_mira-and-tgicl-ass_franssen_31038
SEQUENCE=ATCGATCGCTTTTTTTAAATTACACACTGCAAAAGCGCTTCGGGNGGGTCACTACCACTCTCTTTTCCTTTTTTTTCTCTTCATTTTTTTTTCTATTGTTGAGTATTGTGATGACTTGTTCAAGTGAAAACTTAGCAAAAGCGGTTGGAGGAAAAACCGCTAGAGCTTGTGATAGTTGCATAACAAAGAGAGCACGTTGGTATTGTGCTGCGGATGATGCTTTTCTCTGTCAAGGCTGTGATTCTTCGGTTCATTCTGCTAATTCCTTGGCTAGAAGACATGAAAGAGTTCGTTTGAAAACGGCTTCATATAAACCAGTTGGTGATAATAATGATAATAATAATAATGGATCCATCAATTCTGGTCCATTTTCTGGCTTTACCAAGAAAGCTAGAACACCGAGACAGGGGAAACAGAAGAATAATATTAATGTTGATCTTAATGTTAATGTTAATGTTAATGTTAATGTTGATGTTAATAATCCGTTTCATCTTGTGCCGGAATTGGGATTGGATGAAGTTCAATCGGGTTCGAATGAAGAAAACGAGGAGCAGTTGCTTTACAGGGTGCCGATTTTCGATCCTTTTGTTGCTGAATTATGTACTAGTTCTCCTTCGGTTGGTTCAACCGAAGGAGGACTAGCTGTGATAAGTGGTGTTTCTGCTTTCGCTTCTGATAGAAGCGAAAGCAGAACTCGTTTGGGTGGTGGTGATAGTAATTATGAGATGGAAGGCTTTCACGGGCTTCTTCCATCTGATATTGAATTAGCTGAATTTGCAGCGGATGTCGAGAGTTTATTGGGTCGGGGGTTGGAAAACGAGTGTATGGGAATGGAAGAGCTGGGGTTGATTGATGCGAAATATGAGGATGAGGAATGGGAGTGTAGTGGGAAAGTGAAGGAAGAAGGTGGAGAGATTCATGAAGTTGTTGAGGGAGATGATAATACAAAGGATATGGGAAAAGAATCTTCGTTTGAGTTGAATTTTGATTATGAAACATGTGAAGAGGTTAAGGAAAAGGGTGGTTTGGATATAGAACAAGATGAAAATAAAGATTGCAATAAGGGGAAAAGGAAAATATCTTTACAGTTAGATTATGATGCAGTAATCATTGCATGGGATGGCCAAAAATGTCCATGGACTAATGGTGACAAGCCAAATTTGGACATTGATGAGACCTTGTCTGATTTCATGGGGATTTGTGGAACGGAGATTCAATATCCTTATGGTGAATTTGGTGGATATGGTTGTAATCAAGTGATGGTAGATGGAGGTAGAGAGGCAAGGGTTTCAAGGTACAGAGAGAAGAGAAGAACAAGGTTGTTTTCGAAGAAAATAAGGTACGAGGTTAGGAAGTTGAATGCAGAGAAGAGACCAAGAATGAAAGGGAGGTTTGTGAAGAGGACTTCTTTTGCAGTTCCAACATTTCCTTTGCTAAAATAAATAAGAGTTAGCATTTGTTGCTTTTCCTTACCTCTAATCTTTTTTTTCTTCTTAGTATTTGCTAAAAGTAATAATTTTCTATTTTGTATGAGTTTTCATTTTGTTTTACTTTAGTTTTCTAGTACTTTGTATATATAGACAATTTTGTTTAGTTTATGAAATGGTTTAACATTTATAGATGATAAATAAATGTTAGATCCATGTAGACAATTCTAATAACTAAGCCAACAACTTTAATCATTTTAATGTAAAAAA
TARGET=334,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0263
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=GATGCTTTTCTCTGTCAAGGCT
PRIMER_RIGHT_SEQUENCE=GTAAAGCCAGAAAATGGACCAG
PRIMER_LEFT=215,22
PRIMER_RIGHT=381,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.0483
PRIMER_LEFT_1_PENALTY=0.047586
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=AGTGAAAACTTAGCAAAAGCGG
PRIMER_RIGHT_1_SEQUENCE=GTAAAGCCAGAAAATGGACCAG
PRIMER_LEFT_1=122,22
PRIMER_RIGHT_1=381,22
PRIMER_LEFT_1_TM=59.952
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=260
PRIMER_PAIR_PENALTY_2=0.0531
PRIMER_LEFT_2_PENALTY=0.052366
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=TGCATAACAAAGAGAGCACGTT
PRIMER_RIGHT_2_SEQUENCE=GTAAAGCCAGAAAATGGACCAG
PRIMER_LEFT_2=176,22
PRIMER_RIGHT_2=381,22
PRIMER_LEFT_2_TM=59.948
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.0531
PRIMER_LEFT_3_PENALTY=0.052366
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=GTTGCATAACAAAGAGAGCACG
PRIMER_RIGHT_3_SEQUENCE=GTAAAGCCAGAAAATGGACCAG
PRIMER_LEFT_3=174,22
PRIMER_RIGHT_3=381,22
PRIMER_LEFT_3_TM=59.948
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.0962
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=TCGGTTCATTCTGCTAATTCCT
PRIMER_RIGHT_4_SEQUENCE=GTAAAGCCAGAAAATGGACCAG
PRIMER_LEFT_4=245,22
PRIMER_RIGHT_4=381,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_03792 Ps_contig_mira-and-tgicl-ass_franssen_31048
SEQUENCE=AACTTTATCAACAATTATTACAAATTGATCCCAATTTACTTAAAAGGAACGAATAACAACCCAATAAATGGCCTTACCAAAAATACAATAGCCAAATCTTGAACTAGCTCATAATAAAAGTGTTATACCATTAGTACTACTAATTAAACATGAAGCTAGAGAGAGATACATTGAAACATCCCAAGCAACAACTCTACTAACAAAGTAACAACATTATTATAATACATAATATACACATTCATCCACATGCACCAAATATTAATCAATCCATGTTTGCTGAAAAGCAAACATAGAAACTCAATTAATATATAACTAGTTCATCATATTCATTATTGATGTCAGTGATAATTAGTTGGAAATGGAGTTGCTCCTCCAGCAAAACATGTTGTTCTCTTAACAAAACGACCTTTCATTCTTGGTCTCTTCTCTGCATTCAATTTCCTTACTTCATATCTTATCTTCTTTGCAAACAAACGTGTTCTTCTTTTCTCTCTATACCTTGATACTCTTGCTTCTCTTCCTCCATCTCCATTACTTCCTATTAAGTTCCCTCTTATGCTTCTTATTTCTCCATCTACACCACTTGAACCCAAGAAATTTTGCCATGAATCATCATCGGAGTTGAACTTAGGTGGGTTTGCGGTAGTCCATAGAGAAGGAGAGCCTTGCCTAGACCAAGCACTAATAACATCTTCATAGTTTAGTCTCAAAAATCTCTCTCTTTTTGTTTCCTCTTTAGTAATAACACTCTCACTAACACCAACACTACTATTACTCAAAGGCATAAACTCTTTCTCTTGGTGTACCATTGATTCATCATGATCCATATTCCAATGAAAAACTTCATCACTTGTCATATCAAAAACCGATTCCAAGTCATCAAGTTCTTCATCTTTAACCTTTAATATTTCATTTTTGGATGGATCCATTTCATTCTCTTGTTTATAATCAAACACTTGTTCCGTTGAATTCTCATGAATCTCACTACTCCCAACACCAAGTAAACTTTCAACATTAGCTGCAAACTCAGCAAGATCAACATCAAACTCACAGAAACTATCCAAATCACACATGTCCTCAAAATCAGCAGCATCATTTTTCACATTACAATTCTCTAAATCAAAAGGGTCGACATCATAAACCGGGACGCGACACAGCAACTGCTCTTCCTCCTCCTCCTCAATCTCATTAATATCAACTAGCAATCCCCGTTCTCGATCTTCTTCTTCTTCTTCGCCTCCGAGCTCAGGAACAAGCGGAAGAAAACTAGTATTGAAAAGAATCTTCTCCTTAAGCCGTTGTTGCTGTTGTTGAATCGACGAATTCTTATTATGACGCGGCGTTCTTGCTTTTCTAGTGAATCCACTATGCCACGCTTTTGCTGTTGCTGTCGTCGTAGTCACCTTAGCAGACGCGGTTTGAAGACGAACCCTCTCGTGTCTACTCGCTAACTGATTAGCCGAATGAACCAAGTTGTCACAACCATGACAAAGAAAAGCATCATCAGCAGCGCAAAACCAACGAGCTCTTCGTCTCAAACAGCTATCACAAGCTCTCGCTGTCTTGGCTCCCAAGGCACCTGCATCTGCACCACCACTTTTCATATCGGTTATCATAAAAACGATATAAAAAAATCTATGTTTTTTCTTCTTCCTCCAATATCAAAAGAATGATTCAATTAATGATTTGTGTATGTTGAAAAAACTAGACAGTGTGATGAAGGAGACACTAATGTGAGTAGGGACTGTTGTTTTTCTTGTTCTATGGTTGTTGCCCCCCGCGTACTCTGCGTTG
TARGET=1168,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3126
PRIMER_LEFT_PENALTY=0.059316
PRIMER_RIGHT_PENALTY=0.253332
PRIMER_LEFT_SEQUENCE=CTGCAAACTCAGCAAGATCAAC
PRIMER_RIGHT_SEQUENCE=GCGAAGAAGAAGAAGAAGATCG
PRIMER_LEFT=1018,22
PRIMER_RIGHT=1236,22
PRIMER_LEFT_TM=60.059
PRIMER_RIGHT_TM=59.747
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.3349
PRIMER_LEFT_1_PENALTY=0.081536
PRIMER_RIGHT_1_PENALTY=0.253332
PRIMER_LEFT_1_SEQUENCE=AACATTAGCTGCAAACTCAGCA
PRIMER_RIGHT_1_SEQUENCE=GCGAAGAAGAAGAAGAAGATCG
PRIMER_LEFT_1=1010,22
PRIMER_RIGHT_1=1236,22
PRIMER_LEFT_1_TM=60.082
PRIMER_RIGHT_1_TM=59.747
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=227
PRIMER_PAIR_PENALTY_2=0.3349
PRIMER_LEFT_2_PENALTY=0.081536
PRIMER_RIGHT_2_PENALTY=0.253332
PRIMER_LEFT_2_SEQUENCE=ACATTAGCTGCAAACTCAGCAA
PRIMER_RIGHT_2_SEQUENCE=GCGAAGAAGAAGAAGAAGATCG
PRIMER_LEFT_2=1011,22
PRIMER_RIGHT_2=1236,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=59.747
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.3349
PRIMER_LEFT_3_PENALTY=0.081536
PRIMER_RIGHT_3_PENALTY=0.253332
PRIMER_LEFT_3_SEQUENCE=CAACATTAGCTGCAAACTCAGC
PRIMER_RIGHT_3_SEQUENCE=GCGAAGAAGAAGAAGAAGATCG
PRIMER_LEFT_3=1009,22
PRIMER_RIGHT_3=1236,22
PRIMER_LEFT_3_TM=60.082
PRIMER_RIGHT_3_TM=59.747
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=0.3999
PRIMER_LEFT_4_PENALTY=0.146525
PRIMER_RIGHT_4_PENALTY=0.253332
PRIMER_LEFT_4_SEQUENCE=CACTTGTTCCGTTGAATTCTCA
PRIMER_RIGHT_4_SEQUENCE=GCGAAGAAGAAGAAGAAGATCG
PRIMER_LEFT_4=953,22
PRIMER_RIGHT_4=1236,22
PRIMER_LEFT_4_TM=60.147
PRIMER_RIGHT_4_TM=59.747
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_03793 Ps_contig_mira-and-tgicl-ass_franssen_31048
SEQUENCE=AACTTTATCAACAATTATTACAAATTGATCCCAATTTACTTAAAAGGAACGAATAACAACCCAATAAATGGCCTTACCAAAAATACAATAGCCAAATCTTGAACTAGCTCATAATAAAAGTGTTATACCATTAGTACTACTAATTAAACATGAAGCTAGAGAGAGATACATTGAAACATCCCAAGCAACAACTCTACTAACAAAGTAACAACATTATTATAATACATAATATACACATTCATCCACATGCACCAAATATTAATCAATCCATGTTTGCTGAAAAGCAAACATAGAAACTCAATTAATATATAACTAGTTCATCATATTCATTATTGATGTCAGTGATAATTAGTTGGAAATGGAGTTGCTCCTCCAGCAAAACATGTTGTTCTCTTAACAAAACGACCTTTCATTCTTGGTCTCTTCTCTGCATTCAATTTCCTTACTTCATATCTTATCTTCTTTGCAAACAAACGTGTTCTTCTTTTCTCTCTATACCTTGATACTCTTGCTTCTCTTCCTCCATCTCCATTACTTCCTATTAAGTTCCCTCTTATGCTTCTTATTTCTCCATCTACACCACTTGAACCCAAGAAATTTTGCCATGAATCATCATCGGAGTTGAACTTAGGTGGGTTTGCGGTAGTCCATAGAGAAGGAGAGCCTTGCCTAGACCAAGCACTAATAACATCTTCATAGTTTAGTCTCAAAAATCTCTCTCTTTTTGTTTCCTCTTTAGTAATAACACTCTCACTAACACCAACACTACTATTACTCAAAGGCATAAACTCTTTCTCTTGGTGTACCATTGATTCATCATGATCCATATTCCAATGAAAAACTTCATCACTTGTCATATCAAAAACCGATTCCAAGTCATCAAGTTCTTCATCTTTAACCTTTAATATTTCATTTTTGGATGGATCCATTTCATTCTCTTGTTTATAATCAAACACTTGTTCCGTTGAATTCTCATGAATCTCACTACTCCCAACACCAAGTAAACTTTCAACATTAGCTGCAAACTCAGCAAGATCAACATCAAACTCACAGAAACTATCCAAATCACACATGTCCTCAAAATCAGCAGCATCATTTTTCACATTACAATTCTCTAAATCAAAAGGGTCGACATCATAAACCGGGACGCGACACAGCAACTGCTCTTCCTCCTCCTCCTCAATCTCATTAATATCAACTAGCAATCCCCGTTCTCGATCTTCTTCTTCTTCTTCGCCTCCGAGCTCAGGAACAAGCGGAAGAAAACTAGTATTGAAAAGAATCTTCTCCTTAAGCCGTTGTTGCTGTTGTTGAATCGACGAATTCTTATTATGACGCGGCGTTCTTGCTTTTCTAGTGAATCCACTATGCCACGCTTTTGCTGTTGCTGTCGTCGTAGTCACCTTAGCAGACGCGGTTTGAAGACGAACCCTCTCGTGTCTACTCGCTAACTGATTAGCCGAATGAACCAAGTTGTCACAACCATGACAAAGAAAAGCATCATCAGCAGCGCAAAACCAACGAGCTCTTCGTCTCAAACAGCTATCACAAGCTCTCGCTGTCTTGGCTCCCAAGGCACCTGCATCTGCACCACCACTTTTCATATCGGTTATCATAAAAACGATATAAAAAAATCTATGTTTTTTCTTCTTCCTCCAATATCAAAAGAATGATTCAATTAATGATTTGTGTATGTTGAAAAAACTAGACAGTGTGATGAAGGAGACACTAATGTGAGTAGGGACTGTTGTTTTTCTTGTTCTATGGTTGTTGCCCCCCGCGTACTCTGCGTTG
TARGET=1219,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4059
PRIMER_LEFT_PENALTY=0.331942
PRIMER_RIGHT_PENALTY=0.073989
PRIMER_LEFT_SEQUENCE=TATCAACTAGCAATCCCCGTTC
PRIMER_RIGHT_SEQUENCE=TCTGCTAAGGTGACTACGACGA
PRIMER_LEFT=1192,22
PRIMER_RIGHT=1411,22
PRIMER_LEFT_TM=60.332
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.5251
PRIMER_LEFT_1_PENALTY=0.331942
PRIMER_RIGHT_1_PENALTY=0.193133
PRIMER_LEFT_1_SEQUENCE=TATCAACTAGCAATCCCCGTTC
PRIMER_RIGHT_1_SEQUENCE=AGTAGACACGAGAGGGTTCGTC
PRIMER_LEFT_1=1192,22
PRIMER_RIGHT_1=1444,22
PRIMER_LEFT_1_TM=60.332
PRIMER_RIGHT_1_TM=59.807
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.5251
PRIMER_LEFT_2_PENALTY=0.331942
PRIMER_RIGHT_2_PENALTY=0.193133
PRIMER_LEFT_2_SEQUENCE=TATCAACTAGCAATCCCCGTTC
PRIMER_RIGHT_2_SEQUENCE=GTAGACACGAGAGGGTTCGTCT
PRIMER_LEFT_2=1192,22
PRIMER_RIGHT_2=1443,22
PRIMER_LEFT_2_TM=60.332
PRIMER_RIGHT_2_TM=59.807
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=0.5251
PRIMER_LEFT_3_PENALTY=0.331942
PRIMER_RIGHT_3_PENALTY=0.193133
PRIMER_LEFT_3_SEQUENCE=TATCAACTAGCAATCCCCGTTC
PRIMER_RIGHT_3_SEQUENCE=GAGTAGACACGAGAGGGTTCGT
PRIMER_LEFT_3=1192,22
PRIMER_RIGHT_3=1445,22
PRIMER_LEFT_3_TM=60.332
PRIMER_RIGHT_3_TM=59.807
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.6259
PRIMER_LEFT_4_PENALTY=0.240472
PRIMER_RIGHT_4_PENALTY=0.385416
PRIMER_LEFT_4_SEQUENCE=ATCCAAATCACACATGTCCTCA
PRIMER_RIGHT_4_SEQUENCE=GAATTCGTCGATTCAACAACAG
PRIMER_LEFT_4=1058,22
PRIMER_RIGHT_4=1325,22
PRIMER_LEFT_4_TM=60.240
PRIMER_RIGHT_4_TM=59.615
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_03794 Ps_contig_mira-and-tgicl-ass_franssen_31053
SEQUENCE=AAGCAGGGGCCACATCTCTATGTTCTCTTACACTACAACCTTCACTACTCTCTCTCTCTCTCTCACAGTCTTCTCTCCGATCCGTGGTTTGGTTTCTTCTCGTTTCTTCCAAAATGCTGGCACGTTTAGCCGCGAATCGTCTCAACGAGATCCGTCAGATTTTCCGTCAGCCAGCGAGGGTTTTCTCAACAGCTCTCAACTATCACCTCGACTCTCCGGACAACAACCCCAATCTTCCTTGGGAATTCAACAATGCCAACAAAGAAAAGGTGAAGGAGATTTTATCTCACTATCCATCCAACTATAAACAATCTGCTGTGATCCCTCTGCTGGATCTTGCACAGCAACAGCATGGTGGCTGGCTTCCCGTTTCTGCAATGGATGCGGTGGCTAAGGTTATAGAGGTTGCTCCTATTCGGGTATATGAGGTTGCCACATTTTACTCAATGTTCAATCGAACTAAGGTTGGCAAATATCATCTATTGGTCTGTGGAACTACACCTTGTATGATACGTGGTTCACGAGGTATTGAAGAAGCATTATTGAAACACTTAGGAGTGAAACGAAATGAAGTAACGCAAGATGGTTTCTTTTCTGTTGGTGAAATGGAATGCATGGGATGCTGTGTGAATGCTCCAATGATTACAGTGGCTGATTACTCTAATGGATCAGAAGGATATACTTACAATTATTTTGAAGATGTAACCCCAGAGAAAGTAATTGAGATAGTTGAAAAGCTGAGAAAGGGCGAGAAGCCACCGCACGGCACACAAAATCCACAGCGGATTAGGAGTGGACCTGAAGGGGGGAATACTACATTGTTAGGTGAGCCCAAACCTCCTCCATGCCGTGACCTGGATGCCTGCTGAAGAGTGTGTGATACATTTTGTCTTCAATAATGTGAGGCTTGCACGTATTCGAGCTTGCCTTAATTGGAACATTGTCAAATTATTAGATGCTCAACCAATTTTTGTATTAACCTGCCCAAGTGGCTTCAGTTTAAAATATCTGTTTCATAAAACGCCTGGAATCTGTTGGCTTGTTTATTACTTGACAGTATTATCTATGCCTTTCTGTAGTCTTTACATTACTTAATCATCATCGTGAATGAAATGAAAGTTGTTTAAGGAGTNACAGTGATTGACAAAGAA
TARGET=48,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6328
PRIMER_LEFT_PENALTY=0.396086
PRIMER_RIGHT_PENALTY=0.236697
PRIMER_LEFT_SEQUENCE=GGGCCACATCTCTATGTTCTCT
PRIMER_RIGHT_SEQUENCE=GCATTTTGGAAGAAACGAGAAG
PRIMER_LEFT=6,22
PRIMER_RIGHT=116,22
PRIMER_LEFT_TM=59.604
PRIMER_RIGHT_TM=60.237
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=111
PRIMER_PAIR_PENALTY_1=0.6550
PRIMER_LEFT_1_PENALTY=0.396086
PRIMER_RIGHT_1_PENALTY=0.258908
PRIMER_LEFT_1_SEQUENCE=GGGCCACATCTCTATGTTCTCT
PRIMER_RIGHT_1_SEQUENCE=GGAAGAAACGAGAAGAAACCAA
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=109,22
PRIMER_LEFT_1_TM=59.604
PRIMER_RIGHT_1_TM=59.741
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=104
PRIMER_PAIR_PENALTY_2=0.6550
PRIMER_LEFT_2_PENALTY=0.396086
PRIMER_RIGHT_2_PENALTY=0.258908
PRIMER_LEFT_2_SEQUENCE=GGGCCACATCTCTATGTTCTCT
PRIMER_RIGHT_2_SEQUENCE=TTGGAAGAAACGAGAAGAAACC
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=111,22
PRIMER_LEFT_2_TM=59.604
PRIMER_RIGHT_2_TM=59.741
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=106
PRIMER_PAIR_PENALTY_3=0.7573
PRIMER_LEFT_3_PENALTY=0.396086
PRIMER_RIGHT_3_PENALTY=0.361206
PRIMER_LEFT_3_SEQUENCE=GGGCCACATCTCTATGTTCTCT
PRIMER_RIGHT_3_SEQUENCE=TCGAGGTGATAGTTGAGAGCTG
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=210,22
PRIMER_LEFT_3_TM=59.604
PRIMER_RIGHT_3_TM=59.639
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=0.8113
PRIMER_LEFT_4_PENALTY=0.396086
PRIMER_RIGHT_4_PENALTY=0.415247
PRIMER_LEFT_4_SEQUENCE=GGGCCACATCTCTATGTTCTCT
PRIMER_RIGHT_4_SEQUENCE=TGAGAGCTGTTGAGAAAACCCT
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=197,22
PRIMER_LEFT_4_TM=59.604
PRIMER_RIGHT_4_TM=60.415
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_03795 Ps_contig_mira-and-tgicl-ass_franssen_31055
SEQUENCE=CACATAATACTYYACAAAAACAAAAAACAAATACCCTTAATAATAATACAATATCTAAAATTCTAAATACACAGTACATCATCGAATTGTCAAATTAGAAAAACAAAAAGTACAAGTAAATAATTTCAAGGAAACTCAAATATTTATTTATTACTACTACTCCCTATTCAGACACAAAAAATAAAAATGATTTTTAATTTTCACTTTCACTTGAGTCAGAAGCACCGTGATGATTGACCTTAGCAATCCCTATCCTTGGCGTATCCACAATAGTACAAGCAGAAGACATTTCCATTTGACAAACCCTAACCGCCCCGATCAACTTCTCCGGCAACTCCTCCTCCGTCTGCGCCTCCACAACAAATCCCATATCAATCGTAACACTCGTCACATATCCCAACGCAAGTCGCAGGATAGCATTCGCTATTGCACTACTTCCGATATCAACGTCAATCTCTAAGTAGTTAGGTCCTCTATGATAATGACACGTCAGCGCTTTCCCTAACAAACACGCGCTGTAGTTACCTACAGCCTTTTTCACTATCCACGGTCCTTTCACGATCCGGTTCACTAACTTGAACCGCTGATTTCGAAAGGAGTCGTCTCCTTCGATGAACCGGCCGAGCAACGAACCGGATTGAACCGGATCTTCCGTCGCGTAGTAGAACACCGCGCTGTGGTGCTCCTTACCTGGAATCTGGAGATTCACGGCGAAGATGAAACTCTTCAACGACTTACCGTTGGATTGCGCTCTCTTGAGCGCGTTGGAGATTCGATTATCGGAGCGATTCAACACGTTGTCGAGTTTCGTTGACGATTTGAGCCAGTCCATGCCGGCTGGAGAGAGGAGATATTCACCTGCGGGAGATTTTTGTCGTTTTGTGAAGTAGCTCTGAGAGCGGAGGTTGAAGACATCGCCGGGAGGTGAAGCCCAGCCGTTTGTTCCGGTGTTGAGATCGACGTGGCGAAGAGATCCGCCGTTGATTGTTTCAGCTGTCCAGTGAGAAGAATTATCGGCGGTGGATCCTGAATCGGTGCCGGAGCTCCGGGAACTGTCGTCGGTTGCGTACATCAGTTTGGTTTGGGGATTTGTGTGGAGTGGTTGGGGTTTTGAAGAGTGAAAAAGTTCATTTAATTGTTTTTTCTGTGTTCACTGCACACAGCGAAACACGCCTAAGATATCAGTTCTCTCCTTTTCCCYGCGTACTACATGTGTTATAGTGGAATCCT
TARGET=141,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03796 Ps_contig_mira-and-tgicl-ass_franssen_31058
SEQUENCE=GTTGTTGTTATGTTTGGGTTTAGTTAGTGGNGAAAGACTGATCACTTTTTTGTCTCTTGACTTGGCNGATCTCTTATGTTCTTTGATCTCAGCGTTTTGGAGTGTTTGAGAAGCTATTCACTCTAGTGGAAGAAAATTTATTTATTCTTGGAGCGGCTTTGGTGATATATATATACTAGGTTTTAAGGTTGTCAGGGTGTGTTGGAACAAATGTCGTTCCGGAGTATTGTTCGTGACGTGAGGGATGGGTTTGGAAGTTTATCAAGGCGGAGTTTTGAGGTAAGGCTTCCTGGACATCAGAAGGGGAAGTCGAGAAGTTCGGTCCATGAATTGCAGGATCAGCCTCCTGTTATACAGAATAGCCGGTGGGCGAGTTTACCGCCGGAGCTTCTTCGCGATGTCATTAAGAGATTGGAGTCAAATGAGAATACTTGGCCTAGTCGCAAACATGTGGTTGCGTGTGCTGCAGTGTGCAAGTCGTGGAGAGAAATGTGCAAAGAAATTGTTGGCAGTCTTGAGTACTGTGGGAAGATTACTTTTCCTGTTTCTCTGAAACAGCCCGGGTATAGGGATGGTCCAATCCAGTGTTTCATCAAGAGAGACAAATCTAAGCTGACATACCACCTTTTCCTTTGCCTTAGTCCCGCCTTGCTTGTTGAAAATGGAAAATTTCTTCTCTCTGCTAAACGGACCAGAAGAACGACTTGCACTGAGTATGTTATATCAATGGATGCAGATAACATATCAAGATCTAGCAGCACTTACATCGGAAAGCTTAGGTCTAATTTTCTCGGAACCAAGTTCATAATTTATGATACGCAGCCTCCCTATAACAATTCTCAGTTGGCGGCTCCACCTGGACGCAGCAGCCGAAGATTCTACTCGAAAAAAGTTTCTCCAAAGGTCCCTAGTGGCAACTACAACGTGGCACAGGTTACCTACGAGTTGAATGTCCTCGGCACAAGAGGTCCACGGAGGATGAATTGCACTATGTACTCAATCCCTTCGTCATCCCTCGACCCCGGCGGCGTTGTCCCAGGCCAGCCAGAGCTCATTTCTCGGTCACTCGAGGATTCTTTCCGTAGCATATCCTTTGCCAGATCGATCGATAACTCAACCGAGTTTAGCAGCTGTAGATTCTCGGACATAATGGGAGCTAGCATAATGGGAGATAACAATGAAGACGAGGAGGGGAAGGAGAGACCTCTAGTTCTTAAAAACAAGTCTCCAAGATGGCACGAACAGCTGCAATGCTGGTGCCTTAACTTTAGGGGACGCGTGACCGTTGCGTCCGTCAAGAATTTTCAGCTGATTGCTTCAACACAGCCTGCAGCTGTCGCCCCGCGTGCTACAACAGGACCTGCGCCGTCGCAGCCAGCCCAATCTGACCATGACAAGATTATTCTTCAGTTCGGTAAGGTCGGCGAGGACATGTTTACAATGGACTATCGTTACCCTCTGTCGGCGTTTCAGGCTTTTGCGATATGTTTGACTAGCTTTGACACAAAACTGGCATGTGAATAGTGTGACAAGTAGAATGTAATCTCCATTTTAGGAATTCATAGGATGGTGAAGCTGAACCAAGTCATTGTATGCCTCATAGTTACTCTAAATTGTAAAGTACAAAACAGTCTCTTCCCAATGTCTTTTTTTTCTTCTTTTGTCATTACCCTTTTTTTGTTCTTTGCCCTTTTCTTTTGAATCCTTATGTTTAGTATGTTTTAAAGTTTAAACTCTCACTGTCAAAACTTTGCTGGTGTAGACTTCCATACTTTTTATGGTAAAGATGTAATGAAAAATGTAGTAGCATTCAATGTCGAAT
TARGET=165,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1018
PRIMER_LEFT_PENALTY=0.053348
PRIMER_RIGHT_PENALTY=0.048460
PRIMER_LEFT_SEQUENCE=CTCTTGACTTGGCNGATCTCTT
PRIMER_RIGHT_SEQUENCE=ATAAACTTCCAAACCCATCCCT
PRIMER_LEFT=53,22
PRIMER_RIGHT=261,22
PRIMER_LEFT_TM=59.947
PRIMER_RIGHT_TM=59.952
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.1079
PRIMER_LEFT_1_PENALTY=0.053348
PRIMER_RIGHT_1_PENALTY=0.054514
PRIMER_LEFT_1_SEQUENCE=CTCTTGACTTGGCNGATCTCTT
PRIMER_RIGHT_1_SEQUENCE=TGATAAACTTCCAAACCCATCC
PRIMER_LEFT_1=53,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=59.947
PRIMER_RIGHT_1_TM=60.055
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.2202
PRIMER_LEFT_2_PENALTY=0.171757
PRIMER_RIGHT_2_PENALTY=0.048460
PRIMER_LEFT_2_SEQUENCE=ATCTCAGCGTTTTGGAGTGTTT
PRIMER_RIGHT_2_SEQUENCE=ATAAACTTCCAAACCCATCCCT
PRIMER_LEFT_2=85,22
PRIMER_RIGHT_2=261,22
PRIMER_LEFT_2_TM=60.172
PRIMER_RIGHT_2_TM=59.952
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=0.2630
PRIMER_LEFT_3_PENALTY=0.053348
PRIMER_RIGHT_3_PENALTY=0.209637
PRIMER_LEFT_3_SEQUENCE=CTCTTGACTTGGCNGATCTCTT
PRIMER_RIGHT_3_SEQUENCE=CCTTGATAAACTTCCAAACCCA
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=59.947
PRIMER_RIGHT_3_TM=60.210
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.2854
PRIMER_LEFT_4_PENALTY=0.053348
PRIMER_RIGHT_4_PENALTY=0.232060
PRIMER_LEFT_4_SEQUENCE=CTCTTGACTTGGCNGATCTCTT
PRIMER_RIGHT_4_SEQUENCE=ACAATACTCCGGAACGACATTT
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=229,22
PRIMER_LEFT_4_TM=59.947
PRIMER_RIGHT_4_TM=59.768
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_03797 Ps_contig_mira-and-tgicl-ass_franssen_31059
SEQUENCE=GTTAAGGTCCAAACCGAACGGGAAATTAGGGTCCGACATACAAACACAAACAATTGAATGAATGCACATGCTTTTGTACCATCACACTAATTGAATCACTACACAACAAACATTTTCTTTTCATCACACATAAATGCTGCACACTATTATGAGAAACGTGTTAACCAGAAGTTAACACTAACTCAAAAACGATAGGACAAGCTAGCTTTATTTATTCTTGTTGGTCAACTAAGTATTGACCAACATTCATCAATGCTCACTTTGTCCTTCTTTCAAATTGAGATTTATACAAGCTTTTTCACATTGACTCCATAATGTGCAACTATTGACTGCTACAAAAGCCTAGAAATCCCATAAAATCGTGGTTTTGGATCATATGAATACTCTTCTCAATGCCAATCACACCCATGGTGAGAGCACTTGCAAAAATGACCCCTGAGAGGATGAACACAATGATTGAAGCTCTTCCAAGAATTCTAAGCAATTTTCTTACAAATAACTGTCCCACAAATCCAGCTAGAACAGACACAGTGAAGAGGTATAATGCATAGGGAATAGGGAACCTCTTGAGTAGGTAGAATTCAACCACAGACAAAGATGATGAGAACATCATCACAAATGTTGCTGTTGCACTAGCAACCTGAGGGATAACTCCAATCTCAATGAGAAGAGGTCCCAAAATGAAACCACCTCCAGAGCCAAGTAATCCTCCAACTGTCCCACCTAAGATTCCACACAGTGCACAGAACACAATGTGCAACACAGTCCATTCAATTGAAGCTTCACAGATACATTCCAGGTTTCCTGTACTCATCCTCTTCTTGTAATCCTTGTTCAATTTCACTCCTTCATAGCCAAACACAACAAGCGCAATAGGAAACTGTAAGGAGAAAAGGGCCCAATACCATACACTGCAGACCCTAGCGTTATTCTTAATGATTTGAAGTAGAAGGAAAGAAACCCAAACAACCATCAAAACCAAAATCCTTTTCCATTTAAGGTTAGAACAAAAGATTTGCATAGGTGTCTTCTCTTCTCTAGGTACTAATGGATCATATTCCGTGTCAATTAGAAGTTCGCCACGAGAATTAACAAAAGTTGTTTGCACTTGAGCCATTTCTTTTTTGAGAAGAGTTTCTTCTTTCCACATCTCAGTTCCCTTAAAGAAAGACCTTGAAGAAGTTCCTATGAAGAGAATAATGATAAGGACAGTGATGAGCCAGTAAGGGAAAACAACACTAAGAGAAACACCGACAGTTATACCAAGCATAAGCATAGGTTGAAAAAGAAGAGCTAAATCATAATCCAATATCGGAACATCTTTCGTCGGATGACGAACTCGTAGGTTATAAAACACAGAAGATGTTGATGCTCCCATTATCATACATTTCGAAAGAGCTGCTGCAGACTTGGTATCGAAACCGATTATAAGAGTAAGCATAGGAACAAAAATGCCACCACCACCAACACCACCAACCGTACCAAAAGCAGATCCTAGAAAACCAATAACTGTCGCCAAAGCAAGTCTCCAACTAGCTTTCAGTTCGGGCCAAGTTTTAACGGTTTTGGATCCGTTAAGGGTGTGGAGTTGAAGGGGATTGTGGGGAAGAGATAGAGATGGTTTTTCGTGGTGGTTGGCTGTTTGGGTGAGGTAAAGAGCGGAGAGAATTGCGACGGAGAAAGCGGATACGAGGTAGAGGATGAATGTTTTTGTGGAAAAGGACATGGTTGTCAATTGAGAGTTTCTGGAACAACAACAAAAGACTTAGAGTGAGTAACGTTATGGCATGTGTCGTCTGTCTTTTGAAATATTCTCTTATTAACAAGAATCACATGAGTACAATGTAG
TARGET=1455,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0219
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=ATGAGCCAGTAAGGGAAAACAA
PRIMER_RIGHT_SEQUENCE=TAGGATCTGCTTTTGGTACGGT
PRIMER_LEFT=1214,22
PRIMER_RIGHT=1496,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.0530
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=AGAAGATGTTGATGCTCCCATT
PRIMER_RIGHT_1_SEQUENCE=TAGGATCTGCTTTTGGTACGGT
PRIMER_LEFT_1=1357,22
PRIMER_RIGHT_1=1496,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=140
PRIMER_PAIR_PENALTY_2=0.1311
PRIMER_LEFT_2_PENALTY=0.031769
PRIMER_RIGHT_2_PENALTY=0.099308
PRIMER_LEFT_2_SEQUENCE=AGAAGATGTTGATGCTCCCATT
PRIMER_RIGHT_2_SEQUENCE=CCTTCAACTCCACACCCTTAAC
PRIMER_LEFT_2=1357,22
PRIMER_RIGHT_2=1594,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.1430
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.142283
PRIMER_LEFT_3_SEQUENCE=ATGAGCCAGTAAGGGAAAACAA
PRIMER_RIGHT_3_SEQUENCE=CTAGGATCTGCTTTTGGTACGG
PRIMER_LEFT_3=1214,22
PRIMER_RIGHT_3=1497,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.142
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.1516
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.021195
PRIMER_LEFT_4_SEQUENCE=AGTGATGAGCCAGTAAGGGAAA
PRIMER_RIGHT_4_SEQUENCE=TAGGATCTGCTTTTGGTACGGT
PRIMER_LEFT_4=1210,22
PRIMER_RIGHT_4=1496,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=60.021
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_03798 Ps_contig_mira-and-tgicl-ass_franssen_31063
SEQUENCE=AATTATAAAAGTCCGACANAGAAACACCCCTAAAAGAAGAGAAGAATAAAATCGCGAGGGAGGAGGTAGCAAGTAAAAGCAACACGACACGAGCATGAAGTCTCTATTTATATTCGCGTTTTTCTCTGCGATTTCCATCGCCGCAGCCTCCACAGCCTACCCTTCCATCCCCGGCCTCGATTCCGGCGACTGCTCCCTCACCGGCGACGAAACGAATCTCGTCCCTCCACGCCGCGAAGTCTACGATGACGGACGATTATTCGATATCACACACAGGTTCGTCCCTGAGATGCCGGTTTGGGACTCCAAGGAAGGGTTAGGCCACTTTCTCTGGCTTGCTGATAGCATGAAAAACGGCTCACGCGCTAATGGCTCGGCTATGAAGCTTGGCGTTCACACCGGAACTCATGTTGACGCGCCGGGCCATTTCTATGATAATTACTTCGACGCCGGATTCGATGTTGATACCCTTGATATAGGACTCCTCAACGGTCTTGTCCTTTTGGTTGATGTTCCAAGGGATAACAATATTACTGCTGAAGTTATGAAGTCCTTGTATATCCCTAAAGGTGTAAGGCGTGTGCTCTTCAGAACATTAAATACTGACAGGAAACTTATGTTTAAGAAGGAATTCGACACAAGCTATGTTGGATTCATGGAGGATGGTGCAAAATGGCTGGTGGAGAACACTGACATCAAACTTGTAGGAGTTGATTACTTATCCGTTGCGGCATATGTTCATTCGGTTCAGTCTCATCTTGTTTTTCTGGAAAGCAGGGAAATCATTCTTGTGGAAGGCCTAAAGCTAGACGGTGTCCCAGCAGGAATATATTCACTCAACTGCTTGCCTCTCAGGTTGGTTGGTTCTGAGGCATCACCAATTCGGTGCATTCTGAACCGATGATGAAGTCCGTGAGTTGCACGGTAGCAACATAGCCCGAATGTAGTCAGTGGTATCAACAAGGAGCATTTTGATGCAAACCATACCTATAGTATAGTAATAATCTCAAAGAATTGAGGACTTGCAGATGCACTTGTAATAAACTTGTGTTTTCAACTTTTACCTCAGAAGTAGTCCCCCTTGTGCACCATTTGAACTTGTAGACCCATGCCCATAAATTCTTTATAATTTCATTATATATTAATTATGAGGATTTCTTAATGCATCCTATATGTTTTTTTGGAAATAGAAAAAAAATACATTGTATTT
TARGET=855,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0303
PRIMER_LEFT_PENALTY=0.012470
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=GGTGGAGAACACTGACATCAAA
PRIMER_RIGHT_SEQUENCE=TGACTACATTCGGGCTATGTTG
PRIMER_LEFT=678,22
PRIMER_RIGHT=949,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.0519
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.017846
PRIMER_LEFT_1_SEQUENCE=ATCATTCTTGTGGAAGGCCTAA
PRIMER_RIGHT_1_SEQUENCE=TGACTACATTCGGGCTATGTTG
PRIMER_LEFT_1=781,22
PRIMER_RIGHT_1=949,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.0606
PRIMER_LEFT_2_PENALTY=0.012470
PRIMER_RIGHT_2_PENALTY=0.048131
PRIMER_LEFT_2_SEQUENCE=GGTGGAGAACACTGACATCAAA
PRIMER_RIGHT_2_SEQUENCE=ACTACATTCGGGCTATGTTGCT
PRIMER_LEFT_2=678,22
PRIMER_RIGHT_2=947,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.048
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=270
PRIMER_PAIR_PENALTY_3=0.0822
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.048131
PRIMER_LEFT_3_SEQUENCE=ATCATTCTTGTGGAAGGCCTAA
PRIMER_RIGHT_3_SEQUENCE=ACTACATTCGGGCTATGTTGCT
PRIMER_LEFT_3=781,22
PRIMER_RIGHT_3=947,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=60.048
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=167
PRIMER_PAIR_PENALTY_4=0.0915
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.057411
PRIMER_LEFT_4_SEQUENCE=ATCATTCTTGTGGAAGGCCTAA
PRIMER_RIGHT_4_SEQUENCE=TACAAGTGCATCTGCAAGTCCT
PRIMER_LEFT_4=781,22
PRIMER_RIGHT_4=1038,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_03799 Ps_contig_mira-and-tgicl-ass_franssen_31069
SEQUENCE=TTTAATTGATGAATCTAATAAATAGATATGCAAAGTTTGGTCAATCTCAAGATAATATTAAAATTTATCAAATTTAATTATCTGTTCTCAATTTTTGTCTCTCAGTGATTGATTACTAAAGATAACCATCAATAACTTGGTTAGATGCATTGCCTACAAATCCCAAAGCATCTCTTAAATTTAGTGTGTTTCTCATTGTTCTCCTTCCTATCCCTCCATTGCCCTTTCTCATCATGGCAGCAAATTCTCCATAATCTATTTGTCCATCATTATCTTGATCAATCTCCTTGATCATTTCATCGATATGGATATCATCTAAACCGAAGTCCTTACAAGCTTGTGAAATCTCGTCGATGGTTATGTAACCACTAGCATCTTTGTCAAAATAGGAGAAGGCTGTTAACAAGTTCTCCTCTCTCTCCAGCTTATTTAAATGAACAGTTGCAGCAATGAATTCGCCATAGTCAATTGTTCCGTTGTTATCAATATCCGCAGCGTCCATAAGATCCTGGATTTCAGACTCCATAAGTTCTGATCCTACCCGCTTTAAGCCATCTTTTAACTCATCAAATGTTATGGTTCCACTGCTGTCTGTGTCAATCATCTTGAATAACTCTTTCAGGCCACCAATTTCTTCCTCGGATAGCCGCTCCGCAATAACACGCAAAGCCATCTTTTTCAGTTTATTCATAGCAGAGAATTGCTTCAGACGTGATAAAACTGCAGAGTCAATAGGTTTATCAGGTGCGATGTTGTCATCAACAATCCATGGGTGGCGGAGTACTTCATGTGCCGTAAGCCTTGTTCTCGGATTTTGATCAAGCATTTTCCGGATTAGATCCTTTGCGCTGTCTGAAATGCTAGGCCAAGGCTCAGACTGAAAATCAAGTTTTCCTAATAAAATCTGTCGGAAGATCCCCGGTTCAGTTTCTGCCCAAAATGGTGGCACCCCACTTAATAAGATGTACAAAATAACCCCGGCACTCCACACATCCGATTCAGGGCCATATAGTTTGCGCAAGACCTCCGGTGCAACATAGTATGGGCTCCCAACAACATCAGAAAAAGATTCACCAGGTTTATAAAAAACAGACAAACCAAAATCAGTAGCTTTAAGCTTAGCATCTTCATCAACAGTATCAAAGAGAAAATTCTCCGGTTTAAGATCCCTATGCATAACACCAAGTGAGTGACAAGCCTCAACAACCTCAACAATTGTCTTAATCAAAGTCGCAGCTTGTCTCTCACTATAATGGCCCTTCTGAACAATCCTATCAAAGAGTTCACCTCCTTCACAAAGCTCCATAACAATATGAACAGCAGTGGAATCCTCATAAGTCCCTTCGATTCTAACAACATGTGAATGCTCAGATAAATGATGCATTATCTGAATCTCTCTCCAAACATCTTCGTAATCCTCTTTGCAAAACAGCTTCCGTTTAGGGATTGACTTGCACGCGTACCTTCTGTTTGATGATTTGTGTGTGCAGAGATAGGTTGTACCGAATTGACCTTGACCGAGTTTTCTACCCATTCGGTATATCTCTGTTATCTTCTCGGTTCTGTAGGGTAAAACCCATGCTACTGGTTTCAATGTTATGGTTGGATTCTCTTTCGCCATTTCAAAGATGAAGAGAGAAGAAAAAAAGTGAAATGATGAATAATTGAAGTTGTGTTTGTGTTTTGGAGTTATTATTGTTGTTGTTGTTACAGAATGGTAAGCACGTTACTATGTTGAGGAGAAAAAGTGAGTGATTAAATTNAGGTGGTCGTGTTTTTCGGTCAYCCTNTATTTTCTGTCACTTAGAAACATAAAGATTAAATAAATCTGTGGGCTCGGTCTTTTCTACGAC
TARGET=1696,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03800 Ps_contig_mira-and-tgicl-ass_franssen_31071
SEQUENCE=ATGATCAATATAAGTGGTTGGCAACACGTGGAGTAGATGAAGATGTTTTAGTTCAAAGTCTACCAAAGGATCTTCGAAGAGATATTAAGCGTCATCTCTGTTTGGCTTTAGTGAGGAGGGTTCCACTATTTGAGAGTATGGATGAGAGATTGCTTGATGCCATATGTGAGAGACTGAAACCATGCTTAGTTACAGAGAATACTTATATTGTTCGGGAAGGAGATCCAGTTGATGAGATGCTTTTCATCATACGTGGACGCCTTGAGAGTGTTACTACAGATGGTGGCAGGAGTGGTTTTTTCAACCGCAGTCTTCTAAAAGAGGCTGACTTCTGTGGAGAGGAGCTTTTAACCTGGGCACTGGATCCCAAATCTGGTTCTAATCTTCCAACTTCTACTCGAACTGTTAAGGCATTAACAGAGGTTGAGACTTTTGCCTTGACTGCCGATGAGCTCAAATTTGTCGCTAGTCAGTTTAGGCGTCTTCACAGTAGACAGGTTCAGCACACTTTTCGTTTTTACTCTCAGCAATGGAGAACTTGGGCTGCATGCTTTATTCAAGCAGCATGGCGCAGATATTGTAAAAAGAAAATTATGAAGCTTCGTCAAAAGGAAGAAGAAGAAGCAGACGAGTCAGAAGGAACTCAAGGCAACTCTGGCGGCAGTTCCTCTAGCCTTGGTGCTGCCTTATTGGCCTCAAAGTTTGCCGCACGCACTCTACGAGGTGTACACCGCAATCGACTTGCCAAGACTGCCAGGGAGTTGGTGAAATTACAGAAACCCCCGGAGCCGGACTTTACAGCAGATGATGCAGACTAAAATATCTGTATGTAACTGCTCTTGAAATTCTTGTTTTTATTTACTATTATGACTGAACTTGTGAAAAAGGTCAAAAGCAATGTATCCTCTGTAAATAATTGTATTATAGTATACATTAAACCTAACTGTGTGTCATATTAGGTTTGCACCATAGTTTGAAACATGTTTTGCACTTCAATATAAGTGAAACAATCTCTGTCCAAGATGGACGTGATCGATAGACCAAATTCTATTTCTTTTTTGTACTAGCTCACATTGTTGTARTAATTAGT
TARGET=612,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03801 Ps_contig_mira-and-tgicl-ass_franssen_31074
SEQUENCE=AGCATTAGCATCACTGATTGATAGACTCTGATTCTGAACAGTAACAATAATGAAGTGAGGACAAAGAAGAAAAATAATTGTCCTCTTTCCCTCTTCATTTTTCCTCGACTCATTCTGCTCCCAATTTACACTTCACTGACACACACACCAATTTCCTCCAATCCAATCAACCCTAAAAACCCCATTTTTCTCTAGTGTCGGTGATCCACTTCATTTTCAAACACCATAAAAAAAAATCCCAACCAATTCACACCATTGTTGAATCCTTCAAAAAAACCGCAACAATGTCATCATCGACCAAGCCACCACACTACGCCACAAGCGGTTTGGTGATAGGTTACGCTCTCTGTTCAAGCTTGTTAGCGATAATCAACAAATACGCCATCACTCAATTCAACTACCCTGGTCTCTTAACCGCTTTACAGTACCTCACTTCAGCTCTCGGCGTTTACCTTTTCGGGAAATTAGGGTTTCTTCATCACGATCCTTTCACCATTCCGATCGCCAAGAAATTCTTCCCTGCCGCACTCGTTTTCTTCCTCGCTATCTTCACCAACACGAATCTGCTCCGTCACGCTAACGTTGATACCTTCATTGTTTTCAGATCTCTTACCCCTCTTCTTGTTGCTCTCGCTGATACCGCTTTTCGAGGTCAACCTTCGCCTTCTAACCTTACTTTTTTCTCGCTTGTTGTTATTCTTGCTGGTGCTGTTGGTTACGTTGCTACCGATTCGGGTTTTACTCTTACCGCTTATTCATGGGCTTTTGCTTATTTGGTTACGATTACTACTGAGATGGTTTATATCAAGCATATGGTTATGAGTCTTGGGTTGAATACTTGGGGTTTTGTTCTTTACAATAATGTCTTGTCTTTAATGATTGCTCCTTTCTTTTGGTTTCTTACCGGAGAGAATTTCGAGGTTTCGAATGCGATTAATTCGAGTACCGGGAGTTTGTTTGAGATGAATGCGTTTCTTGCGGTTTCTTTGTCTTGTGTGTTTGGTTTGCTTATCAGTTTCTTTGGATTTGCTGCGAGAAAAGCGGTTTCGGCTACTGCTTTTACGGTTACTGGGGTTGTGAATAAGTTTCTCACGGTGGCTATTAATGTGACGATTTGGGATAAGCATGCTAGTCCTGCTGGTCTGGTTTGTTTGCTTTTTACTATAATTGGGGGAGTTCTTTATCAGCAATCGGTTACTGGGAATGGTTCGCAACAACGGGATGCCGTGGTGGTGACTAAGCAGAGTGATATTGAAAGTAATCTTGTTGGTGATGGTGATTTGGAAGATGAGAGTGAAGTAAAGGGTAAACTTGCTTCTTTATGAGTATTTTGTGTTGTTATTTGTTTTTAGTTTAGTACTTGACTTGGTGTTTAAGCTGTAATGAACTATATGAATGTTGTCTTTTATTAACAACATTCGGAAAATTTTATATAGCTTGAGGTATTATTTTCATAATGATTTGAAATCTAGTGACTTGGCGTGTGCTGTGTTTTGCATATGGAACACACTCTATTCTTGTTTGTAGGCAAGGGTAAATCTAAAACTTGTTTTGGCTCAAGCAATCCATTAGGGAAGTGTGAGTATGAGTATGACCTGTTTCCAATAGGTGTGGTGTTATATAATTATCTTGTTTTGGCTATTCAAACTTATTATATAGTTATCTTGCTTGCTTGCTGCAGAATTTATATATAGAAAAGCTTGTAGTCGCCATTAGCTTGCTTGCTGTTGCTCC
TARGET=139,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3352
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=0.081670
PRIMER_LEFT_SEQUENCE=TCTGCTCCCAATTTACACTTCA
PRIMER_RIGHT_SEQUENCE=AGCTTGAACAGAGAGCGTAACC
PRIMER_LEFT=113,22
PRIMER_RIGHT=356,22
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.3352
PRIMER_LEFT_1_PENALTY=0.253482
PRIMER_RIGHT_1_PENALTY=0.081670
PRIMER_LEFT_1_SEQUENCE=TCTGCTCCCAATTTACACTTCA
PRIMER_RIGHT_1_SEQUENCE=GCTTGAACAGAGAGCGTAACCT
PRIMER_LEFT_1=113,22
PRIMER_RIGHT_1=355,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=60.082
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.3508
PRIMER_LEFT_2_PENALTY=0.269153
PRIMER_RIGHT_2_PENALTY=0.081670
PRIMER_LEFT_2_SEQUENCE=ATTTTTCCTCGACTCATTCTGC
PRIMER_RIGHT_2_SEQUENCE=AGCTTGAACAGAGAGCGTAACC
PRIMER_LEFT_2=96,22
PRIMER_RIGHT_2=356,22
PRIMER_LEFT_2_TM=59.731
PRIMER_RIGHT_2_TM=60.082
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.3508
PRIMER_LEFT_3_PENALTY=0.269153
PRIMER_RIGHT_3_PENALTY=0.081670
PRIMER_LEFT_3_SEQUENCE=ATTTTTCCTCGACTCATTCTGC
PRIMER_RIGHT_3_SEQUENCE=GCTTGAACAGAGAGCGTAACCT
PRIMER_LEFT_3=96,22
PRIMER_RIGHT_3=355,22
PRIMER_LEFT_3_TM=59.731
PRIMER_RIGHT_3_TM=60.082
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.3552
PRIMER_LEFT_4_PENALTY=0.253482
PRIMER_RIGHT_4_PENALTY=0.101680
PRIMER_LEFT_4_SEQUENCE=TCTGCTCCCAATTTACACTTCA
PRIMER_RIGHT_4_SEQUENCE=GAGCGTAACCTATCACCAAACC
PRIMER_LEFT_4=113,22
PRIMER_RIGHT_4=344,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_03802 Ps_contig_mira-and-tgicl-ass_franssen_31074
SEQUENCE=AGCATTAGCATCACTGATTGATAGACTCTGATTCTGAACAGTAACAATAATGAAGTGAGGACAAAGAAGAAAAATAATTGTCCTCTTTCCCTCTTCATTTTTCCTCGACTCATTCTGCTCCCAATTTACACTTCACTGACACACACACCAATTTCCTCCAATCCAATCAACCCTAAAAACCCCATTTTTCTCTAGTGTCGGTGATCCACTTCATTTTCAAACACCATAAAAAAAAATCCCAACCAATTCACACCATTGTTGAATCCTTCAAAAAAACCGCAACAATGTCATCATCGACCAAGCCACCACACTACGCCACAAGCGGTTTGGTGATAGGTTACGCTCTCTGTTCAAGCTTGTTAGCGATAATCAACAAATACGCCATCACTCAATTCAACTACCCTGGTCTCTTAACCGCTTTACAGTACCTCACTTCAGCTCTCGGCGTTTACCTTTTCGGGAAATTAGGGTTTCTTCATCACGATCCTTTCACCATTCCGATCGCCAAGAAATTCTTCCCTGCCGCACTCGTTTTCTTCCTCGCTATCTTCACCAACACGAATCTGCTCCGTCACGCTAACGTTGATACCTTCATTGTTTTCAGATCTCTTACCCCTCTTCTTGTTGCTCTCGCTGATACCGCTTTTCGAGGTCAACCTTCGCCTTCTAACCTTACTTTTTTCTCGCTTGTTGTTATTCTTGCTGGTGCTGTTGGTTACGTTGCTACCGATTCGGGTTTTACTCTTACCGCTTATTCATGGGCTTTTGCTTATTTGGTTACGATTACTACTGAGATGGTTTATATCAAGCATATGGTTATGAGTCTTGGGTTGAATACTTGGGGTTTTGTTCTTTACAATAATGTCTTGTCTTTAATGATTGCTCCTTTCTTTTGGTTTCTTACCGGAGAGAATTTCGAGGTTTCGAATGCGATTAATTCGAGTACCGGGAGTTTGTTTGAGATGAATGCGTTTCTTGCGGTTTCTTTGTCTTGTGTGTTTGGTTTGCTTATCAGTTTCTTTGGATTTGCTGCGAGAAAAGCGGTTTCGGCTACTGCTTTTACGGTTACTGGGGTTGTGAATAAGTTTCTCACGGTGGCTATTAATGTGACGATTTGGGATAAGCATGCTAGTCCTGCTGGTCTGGTTTGTTTGCTTTTTACTATAATTGGGGGAGTTCTTTATCAGCAATCGGTTACTGGGAATGGTTCGCAACAACGGGATGCCGTGGTGGTGACTAAGCAGAGTGATATTGAAAGTAATCTTGTTGGTGATGGTGATTTGGAAGATGAGAGTGAAGTAAAGGGTAAACTTGCTTCTTTATGAGTATTTTGTGTTGTTATTTGTTTTTAGTTTAGTACTTGACTTGGTGTTTAAGCTGTAATGAACTATATGAATGTTGTCTTTTATTAACAACATTCGGAAAATTTTATATAGCTTGAGGTATTATTTTCATAATGATTTGAAATCTAGTGACTTGGCGTGTGCTGTGTTTTGCATATGGAACACACTCTATTCTTGTTTGTAGGCAAGGGTAAATCTAAAACTTGTTTTGGCTCAAGCAATCCATTAGGGAAGTGTGAGTATGAGTATGACCTGTTTCCAATAGGTGTGGTGTTATATAATTATCTTGTTTTGGCTATTCAAACTTATTATATAGTTATCTTGCTTGCTTGCTGCAGAATTTATATATAGAAAAGCTTGTAGTCGCCATTAGCTTGCTTGCTGTTGCTCC
TARGET=1664,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5757
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.541633
PRIMER_LEFT_SEQUENCE=TCAAGCAATCCATTAGGGAAGT
PRIMER_RIGHT_SEQUENCE=AATGGCGACTACAAGCTTTTCT
PRIMER_LEFT=1556,22
PRIMER_RIGHT=1713,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.458
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=0.6303
PRIMER_LEFT_1_PENALTY=0.088662
PRIMER_RIGHT_1_PENALTY=0.541633
PRIMER_LEFT_1_SEQUENCE=CTCAAGCAATCCATTAGGGAAG
PRIMER_RIGHT_1_SEQUENCE=AATGGCGACTACAAGCTTTTCT
PRIMER_LEFT_1=1555,22
PRIMER_RIGHT_1=1713,22
PRIMER_LEFT_1_TM=60.089
PRIMER_RIGHT_1_TM=59.458
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.6503
PRIMER_LEFT_2_PENALTY=0.108684
PRIMER_RIGHT_2_PENALTY=0.541633
PRIMER_LEFT_2_SEQUENCE=TGACCTGTTTCCAATAGGTGTG
PRIMER_RIGHT_2_SEQUENCE=AATGGCGACTACAAGCTTTTCT
PRIMER_LEFT_2=1591,22
PRIMER_RIGHT_2=1713,22
PRIMER_LEFT_2_TM=59.891
PRIMER_RIGHT_2_TM=59.458
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.8465
PRIMER_LEFT_3_PENALTY=0.304841
PRIMER_RIGHT_3_PENALTY=0.541633
PRIMER_LEFT_3_SEQUENCE=TGAAATCTAGTGACTTGGCGTG
PRIMER_RIGHT_3_SEQUENCE=AATGGCGACTACAAGCTTTTCT
PRIMER_LEFT_3=1462,22
PRIMER_RIGHT_3=1713,22
PRIMER_LEFT_3_TM=60.305
PRIMER_RIGHT_3_TM=59.458
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.8814
PRIMER_LEFT_4_PENALTY=0.339815
PRIMER_RIGHT_4_PENALTY=0.541633
PRIMER_LEFT_4_SEQUENCE=ACCTGTTTCCAATAGGTGTGGT
PRIMER_RIGHT_4_SEQUENCE=AATGGCGACTACAAGCTTTTCT
PRIMER_LEFT_4=1593,22
PRIMER_RIGHT_4=1713,22
PRIMER_LEFT_4_TM=59.660
PRIMER_RIGHT_4_TM=59.458
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_03803 Ps_contig_mira-and-tgicl-ass_franssen_31080
SEQUENCE=ATTGAAACAAAAACGAACTCTCTTCTCTTTGTCATTTGGCAAGATCTGAATTTTTCCCCCATCTTTACAGAGAGAGAGAAAGGTGTTGTTTGGTTGGTTCAATTCTACTCTGATCTTCAACTTTCACTCTTTTTCTTCAGATCTTTCACTTCAGGATCAAATTAAGGAATGCCAAGAAGAGAATGGGGAAAAAGGGAAATTGGTTTTCTACAGTGAAGAAAGCTCTCAGCCCTGATTCAAAGAAATCAAGTAAATCAAAGAAGAAATGGTTTGGGAAGCAAAAACTGCAGACTTCGGACCCTTCCGTAGAAGATACGGCACCGCCGCTTCCTCCACCGGAAGATGTTAAATTACCTGATATTGAAAACCAAAACAATCACTATCATGTTCCTGAAATTACAACTGCCGTGGTTAGTCAGGAACCCGGTCCTTCTGTTCCGGCACCAGTTGTTAGGAATGAAGTTTCTGTGGTTGCTCGTTATGCTGGTAAATCAAAAGATGAAGTGGCGGCAATCAAGATTCAAACAGCTTTTCGTGGATACTTGGCACGAAGAGCGTTGCGGGCTTTAAGAGGGTTGGTCAGATTGAAAACATTGATGGAAGGGCCGATTGTAAAACGCCAAGCCATGAGTACGCTCCGCTCAATGCAGACGTTAGCTCGTGTGCAATCTCAGATTCGTTCCAGGAGGGTCAGAATGTTAGAGGAGAATCAGGCTTTGCAAAGACAGCTCTTACAAAAGCATGTAAGAGAGCTCGAGACCCTGCGGATTGGAGAAGAATGGGATGATAGCCTACAATCAAAAGAACAAATTGAAGCCAAATTAATGAGCAAGTACGAGGCTACTATGAGAAGAGAAAGAGCAATGGCTTATGCATTCACTCATCAGCAAAACGGGAAGAGCTCGTCTAGACCTATGAATCCAATGTTCGTGGATCCAACCAATCCTACGTGGGGCTGGAGCTGGCTTGAACGATGGATGGCTGCGCGGCCTTGGGAGAGCCGTGGCCTAATGGATAAAGAACTGAATGACCATTCATCTGTTAAAAGTTCGAGTCGCAGTATTACAGGTGGAGAAATCAGCAAATCATTTGCCCGTTTCCAGCTGAATTCCGAAATACATTCTCCCGCAGCCAGCCAAATTCCAGGCTCGCCGAGCTTTCAGTCAAATTCAACAACACCTAAGCCAGCTTCTGCAGCAGTTGCGAGGAAACTGAAGAAGGCAAGTCCAAAGGGAAGCTGGGTTATGGAAGAAGACTCCAAAAGCATGGCCAGCGTACAGTCAGATCGGTTCCGTAGACACTCTATCGCCGGATCATCAGTTAGGGATGACGAGAGTCTTGCTAGCTCTCCATCAGTTCCGAGCTATATGGTGCCGACTCAATCTGCGAAAGCTAGGTCTAGGACACAAAGTCAGAGTCCATTAGCTAAAGAAAACGGCAAACAAGAGAAGGGGTCCTTTGGGACCGCAAAGAAGCGTCTTTCTTTCCCGGCTTCTCCCGCCAGGCCAAGGCGTCATTCAGGTCCACCAAAGGTAGAAATCGCCGTAAATGCAGAGGTCAGTGTGGACAATGGTGTGGCCAGTTGATTAAATAATAATCAGAGTGAAAGGATTGAAATCATTAAAGTAGGATTGAAGATATATCAGTGAAGAAAATAAAAACAAAAGTTCATTTACATTGAGTGTTCTTTAAATTTATTTATTTGTCCTATTTTTATGTGTATCCTAACATTTTATGTAGGCTTTTGATTTGGGGTGAAAATTGTTTTACTTACCATATATTCATCTATTTGTATTTTTATTTACATTCTCTATCATTTACACTGAGGGGAAGAAATTTGGTTTATGATGATCTTGTGACAAGAAAATGACGTCCTACTTTTCTTTNNTTTTTGTTCACCATAAACTGTAAATTTAAAGCCTCCTGTATTTACTTAATGTAATATAAGTAACTTAAATTTTTAAAATTTTT
TARGET=69,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5464
PRIMER_LEFT_PENALTY=0.491944
PRIMER_RIGHT_PENALTY=0.054491
PRIMER_LEFT_SEQUENCE=GATCTGAATTTTTCCCCCATCT
PRIMER_RIGHT_SEQUENCE=CCTTTTTCCCCATTCTCTTCTT
PRIMER_LEFT=42,22
PRIMER_RIGHT=194,22
PRIMER_LEFT_TM=60.492
PRIMER_RIGHT_TM=59.946
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.5464
PRIMER_LEFT_1_PENALTY=0.491944
PRIMER_RIGHT_1_PENALTY=0.054491
PRIMER_LEFT_1_SEQUENCE=AGATCTGAATTTTTCCCCCATC
PRIMER_RIGHT_1_SEQUENCE=CCTTTTTCCCCATTCTCTTCTT
PRIMER_LEFT_1=41,22
PRIMER_RIGHT_1=194,22
PRIMER_LEFT_1_TM=60.492
PRIMER_RIGHT_1_TM=59.946
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=154
PRIMER_PAIR_PENALTY_2=0.5670
PRIMER_LEFT_2_PENALTY=0.491944
PRIMER_RIGHT_2_PENALTY=0.075027
PRIMER_LEFT_2_SEQUENCE=GATCTGAATTTTTCCCCCATCT
PRIMER_RIGHT_2_SEQUENCE=CTTGATTTCTTTGAATCAGGGC
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=249,22
PRIMER_LEFT_2_TM=60.492
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.5670
PRIMER_LEFT_3_PENALTY=0.491944
PRIMER_RIGHT_3_PENALTY=0.075027
PRIMER_LEFT_3_SEQUENCE=AGATCTGAATTTTTCCCCCATC
PRIMER_RIGHT_3_SEQUENCE=CTTGATTTCTTTGAATCAGGGC
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=60.492
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.6838
PRIMER_LEFT_4_PENALTY=0.491944
PRIMER_RIGHT_4_PENALTY=0.191886
PRIMER_LEFT_4_SEQUENCE=GATCTGAATTTTTCCCCCATCT
PRIMER_RIGHT_4_SEQUENCE=GGCATTCCTTAATTTGATCCTG
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=172,22
PRIMER_LEFT_4_TM=60.492
PRIMER_RIGHT_4_TM=59.808
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=131
=
PRIMER_SEQUENCE_ID=SSR_03804 Ps_contig_mira-and-tgicl-ass_franssen_31080
SEQUENCE=ATTGAAACAAAAACGAACTCTCTTCTCTTTGTCATTTGGCAAGATCTGAATTTTTCCCCCATCTTTACAGAGAGAGAGAAAGGTGTTGTTTGGTTGGTTCAATTCTACTCTGATCTTCAACTTTCACTCTTTTTCTTCAGATCTTTCACTTCAGGATCAAATTAAGGAATGCCAAGAAGAGAATGGGGAAAAAGGGAAATTGGTTTTCTACAGTGAAGAAAGCTCTCAGCCCTGATTCAAAGAAATCAAGTAAATCAAAGAAGAAATGGTTTGGGAAGCAAAAACTGCAGACTTCGGACCCTTCCGTAGAAGATACGGCACCGCCGCTTCCTCCACCGGAAGATGTTAAATTACCTGATATTGAAAACCAAAACAATCACTATCATGTTCCTGAAATTACAACTGCCGTGGTTAGTCAGGAACCCGGTCCTTCTGTTCCGGCACCAGTTGTTAGGAATGAAGTTTCTGTGGTTGCTCGTTATGCTGGTAAATCAAAAGATGAAGTGGCGGCAATCAAGATTCAAACAGCTTTTCGTGGATACTTGGCACGAAGAGCGTTGCGGGCTTTAAGAGGGTTGGTCAGATTGAAAACATTGATGGAAGGGCCGATTGTAAAACGCCAAGCCATGAGTACGCTCCGCTCAATGCAGACGTTAGCTCGTGTGCAATCTCAGATTCGTTCCAGGAGGGTCAGAATGTTAGAGGAGAATCAGGCTTTGCAAAGACAGCTCTTACAAAAGCATGTAAGAGAGCTCGAGACCCTGCGGATTGGAGAAGAATGGGATGATAGCCTACAATCAAAAGAACAAATTGAAGCCAAATTAATGAGCAAGTACGAGGCTACTATGAGAAGAGAAAGAGCAATGGCTTATGCATTCACTCATCAGCAAAACGGGAAGAGCTCGTCTAGACCTATGAATCCAATGTTCGTGGATCCAACCAATCCTACGTGGGGCTGGAGCTGGCTTGAACGATGGATGGCTGCGCGGCCTTGGGAGAGCCGTGGCCTAATGGATAAAGAACTGAATGACCATTCATCTGTTAAAAGTTCGAGTCGCAGTATTACAGGTGGAGAAATCAGCAAATCATTTGCCCGTTTCCAGCTGAATTCCGAAATACATTCTCCCGCAGCCAGCCAAATTCCAGGCTCGCCGAGCTTTCAGTCAAATTCAACAACACCTAAGCCAGCTTCTGCAGCAGTTGCGAGGAAACTGAAGAAGGCAAGTCCAAAGGGAAGCTGGGTTATGGAAGAAGACTCCAAAAGCATGGCCAGCGTACAGTCAGATCGGTTCCGTAGACACTCTATCGCCGGATCATCAGTTAGGGATGACGAGAGTCTTGCTAGCTCTCCATCAGTTCCGAGCTATATGGTGCCGACTCAATCTGCGAAAGCTAGGTCTAGGACACAAAGTCAGAGTCCATTAGCTAAAGAAAACGGCAAACAAGAGAAGGGGTCCTTTGGGACCGCAAAGAAGCGTCTTTCTTTCCCGGCTTCTCCCGCCAGGCCAAGGCGTCATTCAGGTCCACCAAAGGTAGAAATCGCCGTAAATGCAGAGGTCAGTGTGGACAATGGTGTGGCCAGTTGATTAAATAATAATCAGAGTGAAAGGATTGAAATCATTAAAGTAGGATTGAAGATATATCAGTGAAGAAAATAAAAACAAAAGTTCATTTACATTGAGTGTTCTTTAAATTTATTTATTTGTCCTATTTTTATGTGTATCCTAACATTTTATGTAGGCTTTTGATTTGGGGTGAAAATTGTTTTACTTACCATATATTCATCTATTTGTATTTTTATTTACATTCTCTATCATTTACACTGAGGGGAAGAAATTTGGTTTATGATGATCTTGTGACAAGAAAATGACGTCCTACTTTTCTTTNNTTTTTGTTCACCATAAACTGTAAATTTAAAGCCTCCTGTATTTACTTAATGTAATATAAGTAACTTAAATTTTTAAAATTTTT
TARGET=1693,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5315
PRIMER_LEFT_PENALTY=0.338015
PRIMER_RIGHT_PENALTY=0.193502
PRIMER_LEFT_SEQUENCE=GTAAATGCAGAGGTCAGTGTGG
PRIMER_RIGHT_SEQUENCE=CCCCAAATCAAAAGCCTACATA
PRIMER_LEFT=1544,22
PRIMER_RIGHT=1754,22
PRIMER_LEFT_TM=59.662
PRIMER_RIGHT_TM=60.194
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=0.5532
PRIMER_LEFT_1_PENALTY=0.338015
PRIMER_RIGHT_1_PENALTY=0.215198
PRIMER_LEFT_1_SEQUENCE=GTAAATGCAGAGGTCAGTGTGG
PRIMER_RIGHT_1_SEQUENCE=AACCAAATTTCTTCCCCTCAGT
PRIMER_LEFT_1=1544,22
PRIMER_RIGHT_1=1842,22
PRIMER_LEFT_1_TM=59.662
PRIMER_RIGHT_1_TM=60.215
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=299
PRIMER_PAIR_PENALTY_2=0.6529
PRIMER_LEFT_2_PENALTY=0.459412
PRIMER_RIGHT_2_PENALTY=0.193502
PRIMER_LEFT_2_SEQUENCE=AAGGTAGAAATCGCCGTAAATG
PRIMER_RIGHT_2_SEQUENCE=CCCCAAATCAAAAGCCTACATA
PRIMER_LEFT_2=1529,22
PRIMER_RIGHT_2=1754,22
PRIMER_LEFT_2_TM=59.541
PRIMER_RIGHT_2_TM=60.194
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.6628
PRIMER_LEFT_3_PENALTY=0.469251
PRIMER_RIGHT_3_PENALTY=0.193502
PRIMER_LEFT_3_SEQUENCE=CAAAGGTAGAAATCGCCGTAAA
PRIMER_RIGHT_3_SEQUENCE=CCCCAAATCAAAAGCCTACATA
PRIMER_LEFT_3=1527,22
PRIMER_RIGHT_3=1754,22
PRIMER_LEFT_3_TM=60.469
PRIMER_RIGHT_3_TM=60.194
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=0.8047
PRIMER_LEFT_4_PENALTY=0.611210
PRIMER_RIGHT_4_PENALTY=0.193502
PRIMER_LEFT_4_SEQUENCE=CAGGTCCACCAAAGGTAGAAAT
PRIMER_RIGHT_4_SEQUENCE=CCCCAAATCAAAAGCCTACATA
PRIMER_LEFT_4=1518,22
PRIMER_RIGHT_4=1754,22
PRIMER_LEFT_4_TM=59.389
PRIMER_RIGHT_4_TM=60.194
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_03805 Ps_contig_mira-and-tgicl-ass_franssen_31081
SEQUENCE=CCGGTAAACGGGTTAGGTTTGGGGGGGGTAAGGTTTTACCGTTTACGGGTTTTTGGTAAAGGAAAAATTACTAAAACCCAAAAAACTAATTAAAGAAGACAGGAGGCTGCAGCAAAACGCGAGAGAGCCATGGCATATGCTCTAGCTCATCAGTGGCAGGCAGGATCAAGGCAGCTTGCCGCTTCTTCAGGGTTTGAACCCGATAAAAGTAGTTGGGGTTGGAATTGGTTGGAAAGATGGATGGCTGTACGTCCTTGGGAGAATCGCTTTCTTGACATTAATACGAAAGATGGAGTGAAGGTAGACGAAAATGATGTTATGGATGGTAGTAGCAATGGAATCAGACCTCAGTTAAAGCCTAATAATGCGAAATCAAATCTGCCAAACATCCATCCAAGTGTTGTCAGTCACAAAACTGGCCCCTCTCTCTCTGATGGCTGTGATTCTTCTTCCACTAGTAAGTCAGCAGGTTTGCTCGAGACATCAAACACACAATCTGTTAAACCAAAATCTAATGCAAATGTTCAAATTCCCGTTGAAGAAAACAACACAAAATCTGGCTTTCCAAGATCTCAAAGTAATCCAAAGGAGAGAAACTCACAGGTAGATAAACAGACGAAAAAGCGGTTATCTCTTCCTAACAATGCGGGTTGTTTCCGGGGCTCAAACGAACAAATATCCTACCAGAACTAATGTGAAAGGGACACGCAGCACACAAAAGCCTATAAGGAATAAACCTAAGCTCAATGAAGACGGGAATAGGAATCTTACTAAATCCGTTCCACAGCAAACGGGTGTGTAACTTATAAGTATTAAGAAACTACTCCATTCTCCCTTTGACATCGCGATATGACATTTGTCTGTGAAATGTAATGCATGGTGCAAAGCGATGAGGGGTCGCAACCACAATGTACATGTTCCTCTTTTCTCTTCTACGAAGACATTGGTGTGGTTTGATTGTTGAGATTGGCTTGGGTTTTGTGAGGTGGATACACACATCTGTTGTAATTATATGCATATTTGTTGTTGTGAGCTATATGATAATGAATGCTGTATAATATTTATGTTCAAATGTACAACTTTCAGAGTGCCATTGCCATCCATTTCATTGACTATTGCCAAGTTGAAAGGTAGTATACTATTTTTGTTCAGAAACT
TARGET=423,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1842
PRIMER_LEFT_PENALTY=0.109200
PRIMER_RIGHT_PENALTY=0.075027
PRIMER_LEFT_SEQUENCE=CATCCATCCAAGTGTTGTCAGT
PRIMER_RIGHT_SEQUENCE=GATCTTGGAAAGCCAGATTTTG
PRIMER_LEFT=386,22
PRIMER_RIGHT=571,22
PRIMER_LEFT_TM=59.891
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=186
PRIMER_PAIR_PENALTY_1=0.1842
PRIMER_LEFT_1_PENALTY=0.109200
PRIMER_RIGHT_1_PENALTY=0.075027
PRIMER_LEFT_1_SEQUENCE=ACATCCATCCAAGTGTTGTCAG
PRIMER_RIGHT_1_SEQUENCE=GATCTTGGAAAGCCAGATTTTG
PRIMER_LEFT_1=385,22
PRIMER_RIGHT_1=571,22
PRIMER_LEFT_1_TM=59.891
PRIMER_RIGHT_1_TM=60.075
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.2141
PRIMER_LEFT_2_PENALTY=0.139083
PRIMER_RIGHT_2_PENALTY=0.075027
PRIMER_LEFT_2_SEQUENCE=AGCAATGGAATCAGACCTCAGT
PRIMER_RIGHT_2_SEQUENCE=GATCTTGGAAAGCCAGATTTTG
PRIMER_LEFT_2=330,22
PRIMER_RIGHT_2=571,22
PRIMER_LEFT_2_TM=60.139
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.3161
PRIMER_LEFT_3_PENALTY=0.109200
PRIMER_RIGHT_3_PENALTY=0.206859
PRIMER_LEFT_3_SEQUENCE=CATCCATCCAAGTGTTGTCAGT
PRIMER_RIGHT_3_SEQUENCE=CCGCTTTTTCGTCTGTTTATCT
PRIMER_LEFT_3=386,22
PRIMER_RIGHT_3=626,22
PRIMER_LEFT_3_TM=59.891
PRIMER_RIGHT_3_TM=59.793
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=0.3161
PRIMER_LEFT_4_PENALTY=0.109200
PRIMER_RIGHT_4_PENALTY=0.206859
PRIMER_LEFT_4_SEQUENCE=ACATCCATCCAAGTGTTGTCAG
PRIMER_RIGHT_4_SEQUENCE=CCGCTTTTTCGTCTGTTTATCT
PRIMER_LEFT_4=385,22
PRIMER_RIGHT_4=626,22
PRIMER_LEFT_4_TM=59.891
PRIMER_RIGHT_4_TM=59.793
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_03806 Ps_contig_mira-and-tgicl-ass_franssen_31085
SEQUENCE=AAGCATGGTATCAACGCANAGGAAACAAATTGTTACAAGTGGAGTGTGAGACAGCGCATCTAATCTCTAATATCTTGTTTTGTGCTAAATAGATATTTGTTAAACAGTCTTTAACCTCATCCCAACTAACAACAACTTGCTTCTTTATTTAATCATTCCATCTTCTTTCTTCGTAGTCTTCTCTTCTTTTCTCTCCTCTCTCGCACACAGATTCACATTATTCAACTCTATCATCATCATCATCATCATGTATCGTCCTCCTTCGTCTTCTGGTTCTTCTTCTTCCTCTTCTCAGCAACAACAGAGCTCCATAAGTCAAACAGGTCTCACAAGATACGGTTCTGCACCCGGCTCTCTCCTCACCAGCACCGTCGATGCTGTTCTAGCAGGATCGCGCCTTCTCCCGGGGACTGGACATTATTTCTCCGGCGACTCCGCTCACCAGCAACACCACCAACACCAGCAGCAACAACAGCAACAGCAACAGCAGAAATCTTCTTACGAAGGCTTTGATGGATCGTCTTTGGTCCGTCAGAAAAGCTCTCCCGCCGGTTTCCTCAACCATCTGGCCACTCTCAATCATAATAATAGCGCGGGGTTCACAATCACGCGAGGCGGAAATGGAGGATCGAGATTGAAGTCTGAATTGAGCTTCACGGGAGGAGGACAAGGGCAAGAATGTCTTTCTAGAATATCCGAAAATAGTGTTGATTATGCTGCAGCAGTAGCGGGAGGAAATGGATCATTGCATAACAGTAACTGGGGTGGAGGATCTGATAATAATAATAATAATAATAACAATAATAACAATAACAATTCAAATTCTATAGTATTTTCTTCTTCTGTTTCTCAAAATCAATCTAATAATAAGCGTTCGAGTAGAAACGACGACGATCCCGACCTTCTTCTTCATTGCCTCAACGCCCTCGAAACTCAGTATAGTCTCCCACAAACTTCTATGGAAATGGACAAGCTTATGCATATTCCTCAAGATTCCGTCCCTTGTAAAATCCGCGCTAAGCGTGGCTGTGCCACTCATCCTCGAAGCATTGCTGAACGGGAGAGAAGAACTAGAATAAGTGGAAAGTTGAAGAAATTGCAGGATCTTGTGCCCAATATGGATAAGCAAACAAGTTATTCAGACATGCTAGATTTGGCAGTTCAACATATCAAAGGTCTTCAAACTCAAGTCCAGAAGCTTCATGAAGATCTTGAGAATTGCACTTGCGGATGTAAACAAAATACATAAACTTACTATGTTTTATGAGAATCTATGGCACTGCATAGAAGTCAAGGTTTTGATGGAAACTGATTTTTGTACCTTCCAACTTTAAACGAATAATGAAGGAAATTGTACATACAGTGTATGTTATAATCTACCTGTGGTAGACATAGTCTAGAAATATCTATTCCTTTTCTTAATTTGATAGTTGTATTTGAAAGTAGAAGTTGATTTTCTTTTAAACTGGCTTTATTAGTTTRRNTTTGACGCG
TARGET=230,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03807 Ps_contig_mira-and-tgicl-ass_franssen_31085
SEQUENCE=AAGCATGGTATCAACGCANAGGAAACAAATTGTTACAAGTGGAGTGTGAGACAGCGCATCTAATCTCTAATATCTTGTTTTGTGCTAAATAGATATTTGTTAAACAGTCTTTAACCTCATCCCAACTAACAACAACTTGCTTCTTTATTTAATCATTCCATCTTCTTTCTTCGTAGTCTTCTCTTCTTTTCTCTCCTCTCTCGCACACAGATTCACATTATTCAACTCTATCATCATCATCATCATCATGTATCGTCCTCCTTCGTCTTCTGGTTCTTCTTCTTCCTCTTCTCAGCAACAACAGAGCTCCATAAGTCAAACAGGTCTCACAAGATACGGTTCTGCACCCGGCTCTCTCCTCACCAGCACCGTCGATGCTGTTCTAGCAGGATCGCGCCTTCTCCCGGGGACTGGACATTATTTCTCCGGCGACTCCGCTCACCAGCAACACCACCAACACCAGCAGCAACAACAGCAACAGCAACAGCAGAAATCTTCTTACGAAGGCTTTGATGGATCGTCTTTGGTCCGTCAGAAAAGCTCTCCCGCCGGTTTCCTCAACCATCTGGCCACTCTCAATCATAATAATAGCGCGGGGTTCACAATCACGCGAGGCGGAAATGGAGGATCGAGATTGAAGTCTGAATTGAGCTTCACGGGAGGAGGACAAGGGCAAGAATGTCTTTCTAGAATATCCGAAAATAGTGTTGATTATGCTGCAGCAGTAGCGGGAGGAAATGGATCATTGCATAACAGTAACTGGGGTGGAGGATCTGATAATAATAATAATAATAATAACAATAATAACAATAACAATTCAAATTCTATAGTATTTTCTTCTTCTGTTTCTCAAAATCAATCTAATAATAAGCGTTCGAGTAGAAACGACGACGATCCCGACCTTCTTCTTCATTGCCTCAACGCCCTCGAAACTCAGTATAGTCTCCCACAAACTTCTATGGAAATGGACAAGCTTATGCATATTCCTCAAGATTCCGTCCCTTGTAAAATCCGCGCTAAGCGTGGCTGTGCCACTCATCCTCGAAGCATTGCTGAACGGGAGAGAAGAACTAGAATAAGTGGAAAGTTGAAGAAATTGCAGGATCTTGTGCCCAATATGGATAAGCAAACAAGTTATTCAGACATGCTAGATTTGGCAGTTCAACATATCAAAGGTCTTCAAACTCAAGTCCAGAAGCTTCATGAAGATCTTGAGAATTGCACTTGCGGATGTAAACAAAATACATAAACTTACTATGTTTTATGAGAATCTATGGCACTGCATAGAAGTCAAGGTTTTGATGGAAACTGATTTTTGTACCTTCCAACTTTAAACGAATAATGAAGGAAATTGTACATACAGTGTATGTTATAATCTACCTGTGGTAGACATAGTCTAGAAATATCTATTCCTTTTCTTAATTTGATAGTTGTATTTGAAAGTAGAAGTTGATTTTCTTTTAAACTGGCTTTATTAGTTTRRNTTTGACGCG
TARGET=274,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03808 Ps_contig_mira-and-tgicl-ass_franssen_31085
SEQUENCE=AAGCATGGTATCAACGCANAGGAAACAAATTGTTACAAGTGGAGTGTGAGACAGCGCATCTAATCTCTAATATCTTGTTTTGTGCTAAATAGATATTTGTTAAACAGTCTTTAACCTCATCCCAACTAACAACAACTTGCTTCTTTATTTAATCATTCCATCTTCTTTCTTCGTAGTCTTCTCTTCTTTTCTCTCCTCTCTCGCACACAGATTCACATTATTCAACTCTATCATCATCATCATCATCATGTATCGTCCTCCTTCGTCTTCTGGTTCTTCTTCTTCCTCTTCTCAGCAACAACAGAGCTCCATAAGTCAAACAGGTCTCACAAGATACGGTTCTGCACCCGGCTCTCTCCTCACCAGCACCGTCGATGCTGTTCTAGCAGGATCGCGCCTTCTCCCGGGGACTGGACATTATTTCTCCGGCGACTCCGCTCACCAGCAACACCACCAACACCAGCAGCAACAACAGCAACAGCAACAGCAGAAATCTTCTTACGAAGGCTTTGATGGATCGTCTTTGGTCCGTCAGAAAAGCTCTCCCGCCGGTTTCCTCAACCATCTGGCCACTCTCAATCATAATAATAGCGCGGGGTTCACAATCACGCGAGGCGGAAATGGAGGATCGAGATTGAAGTCTGAATTGAGCTTCACGGGAGGAGGACAAGGGCAAGAATGTCTTTCTAGAATATCCGAAAATAGTGTTGATTATGCTGCAGCAGTAGCGGGAGGAAATGGATCATTGCATAACAGTAACTGGGGTGGAGGATCTGATAATAATAATAATAATAATAACAATAATAACAATAACAATTCAAATTCTATAGTATTTTCTTCTTCTGTTTCTCAAAATCAATCTAATAATAAGCGTTCGAGTAGAAACGACGACGATCCCGACCTTCTTCTTCATTGCCTCAACGCCCTCGAAACTCAGTATAGTCTCCCACAAACTTCTATGGAAATGGACAAGCTTATGCATATTCCTCAAGATTCCGTCCCTTGTAAAATCCGCGCTAAGCGTGGCTGTGCCACTCATCCTCGAAGCATTGCTGAACGGGAGAGAAGAACTAGAATAAGTGGAAAGTTGAAGAAATTGCAGGATCTTGTGCCCAATATGGATAAGCAAACAAGTTATTCAGACATGCTAGATTTGGCAGTTCAACATATCAAAGGTCTTCAAACTCAAGTCCAGAAGCTTCATGAAGATCTTGAGAATTGCACTTGCGGATGTAAACAAAATACATAAACTTACTATGTTTTATGAGAATCTATGGCACTGCATAGAAGTCAAGGTTTTGATGGAAACTGATTTTTGTACCTTCCAACTTTAAACGAATAATGAAGGAAATTGTACATACAGTGTATGTTATAATCTACCTGTGGTAGACATAGTCTAGAAATATCTATTCCTTTTCTTAATTTGATAGTTGTATTTGAAAGTAGAAGTTGATTTTCTTTTAAACTGGCTTTATTAGTTTRRNTTTGACGCG
TARGET=777,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03809 Ps_contig_mira-and-tgicl-ass_franssen_31090
SEQUENCE=CCTATCCCCTGTGTGCCTTGCCTATTGTTGCGTGTCGGAGCAAGCCCTATAAGTTCCATCAAACATGCAGGAGGTCCTTGGGAACTTGGACTTACGGAATCTCACCAGACACTCGTTGCAAATGGACTCAGAGAAAGGGTTATTCTCAGAGTTGATGGTGGATTTAGAAGTGGAGTTGATGTCATGATGGCAGCAATAATGGGTGCTGATGAATATGGATTTGGTTCAGTGGCAATGATTGCCACTGGGTGTGTTATGGCTCGCATTTGCCACACCAATAATTGTCCAGTTGGTGTTGCCAGTCAGAGGGAAGAGTTGCGTGCACGCTTTCCTGGTGTTCCCGGTGATCTTGTCAACCTCTTTTTATATGTAGCTGAGGAGGTAAGAGGCACTTTGGCACAGCTGGGGTATGAGAAGTTAGATGATATAATTGGCCAAACAGACTTGCTGAGACCAAGAGACATATCTCTTGTGAAAACTCAGCATCTAGATCTGAGTTATATCCTCTCTAGTGTTGGGTTACCTAAATGGAGCAGTACAGAAATCCGGAATCAGGAGCCACATACAAATGGTCCTGTTCTGGATGATGGTTTGCTAGCAGACGCAGAGATTGCAGATGCCATTGAAAATGAAAAAGCAGTCAGTAAGACTGTAAATATATACAATGTTGATCGTGCTGTCTGTGGTCGGATAGCTGGTGTTATTGCAAAGAAATACGGTGATACTGGTTTTGCTGGACAGTTGAATATAACATTTACCGGAAGTGCTGGGCAGTCATTTGGATGCTTTCTGACTCCAGGAATGAATATTCGCCTTGTAGGAGAAGCTAATGATTATGTTGGAAAGGGAATGGCTGGAGGAGAATTGGTTGTCACTCCAGTTGACAAGACAGGGTTCCAACCTGAGGATGCAGCTATTGTGGGTAACACTTGCTTGTATGGGGCAACAGGAGGGCAGGTGTTTGTAAGAGGGAAAGCTGGGGAAAGGTTTGCTGTCAGAAACTCACTTGCTGAAGCTGTAGTTGAAGGCACAGGAGATCTATTTGTTGTGAGTACATGACGTGGTGGCTTGGTTGGTTGGTTACTACCGTAAAGGGAAAAGGTAGGGAAAGTAAAGTTGGTA
TARGET=1069,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6794
PRIMER_LEFT_PENALTY=0.020747
PRIMER_RIGHT_PENALTY=0.658607
PRIMER_LEFT_SEQUENCE=GAGGAGAATTGGTTGTCACTCC
PRIMER_RIGHT_SEQUENCE=TTCCCTACCTTTTCCCTTTACG
PRIMER_LEFT=856,22
PRIMER_RIGHT=1108,22
PRIMER_LEFT_TM=59.979
PRIMER_RIGHT_TM=60.659
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.6833
PRIMER_LEFT_1_PENALTY=0.024665
PRIMER_RIGHT_1_PENALTY=0.658607
PRIMER_LEFT_1_SEQUENCE=GATGCAGCTATTGTGGGTAACA
PRIMER_RIGHT_1_SEQUENCE=TTCCCTACCTTTTCCCTTTACG
PRIMER_LEFT_1=906,22
PRIMER_RIGHT_1=1108,22
PRIMER_LEFT_1_TM=60.025
PRIMER_RIGHT_1_TM=60.659
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.7322
PRIMER_LEFT_2_PENALTY=0.073614
PRIMER_RIGHT_2_PENALTY=0.658607
PRIMER_LEFT_2_SEQUENCE=ACTCCAGTTGACAAGACAGGGT
PRIMER_RIGHT_2_SEQUENCE=TTCCCTACCTTTTCCCTTTACG
PRIMER_LEFT_2=873,22
PRIMER_RIGHT_2=1108,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=60.659
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=7.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.7389
PRIMER_LEFT_3_PENALTY=0.080323
PRIMER_RIGHT_3_PENALTY=0.658607
PRIMER_LEFT_3_SEQUENCE=TGGGTAACACTTGCTTGTATGG
PRIMER_RIGHT_3_SEQUENCE=TTCCCTACCTTTTCCCTTTACG
PRIMER_LEFT_3=919,22
PRIMER_RIGHT_3=1108,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=60.659
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=7.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=190
PRIMER_PAIR_PENALTY_4=0.8169
PRIMER_LEFT_4_PENALTY=0.158297
PRIMER_RIGHT_4_PENALTY=0.658607
PRIMER_LEFT_4_SEQUENCE=AACCTGAGGATGCAGCTATTGT
PRIMER_RIGHT_4_SEQUENCE=TTCCCTACCTTTTCCCTTTACG
PRIMER_LEFT_4=898,22
PRIMER_RIGHT_4=1108,22
PRIMER_LEFT_4_TM=60.158
PRIMER_RIGHT_4_TM=60.659
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_03810 Ps_contig_mira-and-tgicl-ass_franssen_31111
SEQUENCE=AATAGAAAAAAAATGAGGGGTGATGGTTTRCTATTCTATATTTCAATACGATAATGGTATGACGTAGATAATTCCTTTCTTCCGTCGATCGATAACCACATCAAAATTGTTTTTTTTCCAAATCGCTAAATCGGTAATCATTCTCATCATCCCAATCCAATTCTCAGAGCAAGCAACTCCGATCCGATTCTTTTTGCAATCGTAACAGCAACAACGCTTTCTTCCAGGTGTGATTAAATTAATTTGGGAAGTAGATAGTAGTGCTAGATTGGGAAGAGAGAAGATGGGGGCAGTGATGTCGAGATTCTGGTTCATGTTATTCCCTGCTAAAGAGTATAAGATTGTTGTTGTTGGGTTGGATAACGCTGGTAAAACTACAACCCTTTATAAATTGCATCTTGGTGAGGTTGTAACTACCAACCCTACTGTTGGCAGCAATGTCGAAGAGCTCGTTTACAAGAACATACGATTCGAGGTATGGGATCTTGGTGGACAAGAAAGACTCCGGACATCATGGGCAACGTATTACCGAGGAACTCATGCTGTAATTGCAGTGATAGACAGCAGCGATAGAGCCAGAATCTCTATAATGAAGGATGAACTCTTTAGGTTGCTGAGTCATGAAGATTTGCAGCATTCTGTCATCCTTGTCTTTGCTAATAAACAAGATATCAAAGATGCTATGACTCCTGCTGAGATTACTGATGCAATGTCTCTTCACAGCATCAAGGATCATGATTGGCATATACAAGCTTGCAGCGCCCTCTCAGGAGAAGGCCTTTATGATGGTCTGGGTTGGATTGCCCAGCGAGTAACCGGCAAAGCATGAGGTTGGGTTGCTGGAAACTAAGGATGACATTTATTTTAATGAATTGTATTGTTGTGATAAGCGACAAAAATATATGTATCATTTATATTTCAAAGAATCAAGGCAAGTTAGTGTGTGTGGATGAATTGCTGTGCAGAAAAAGATTATTCAGTAATCTATCAAGTCACACACATTTTCCCCAAATTGTTATAGAAAGAAGCCTCTGTAGTCTGTACTGCATTGCACCTTGGCCACTTCCTTTTTCTGTTTGCTGCTGCTTCCTCTCTTGAATACATCTTATTTTTCTGCCTGTGCCTTCCCCACATTGTTGCCTTTATACTAACAGTGATTGGTTTAG
TARGET=342,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03811 Ps_contig_mira-and-tgicl-ass_franssen_31119
SEQUENCE=AAGACAGTGGGTAGTTCAACGACAGAGTANGTCGGGACAACAACAACAAAAACAAACATCCTCAAAATGAGTCTTAGGTGTTTCATTTTCTCTCTGTTAATGTTCACTTTCCATGCGGCGAGTGGCAGTTATATTCCAAACAAAAAACATTCGAATTACCGCAGCCTCCCGAACCAATTTCTGATTCCTCATAACATTGCTCGGGCCGCTGTGGGGTTACATCCCCTAGTATGGGACGAAAAGCTAACTCATTACGCGCAATGGTACGCGAACGAACGGCGTAACGACTGCGCGTTGGAGCATTCTAACGGCCCGTACGGCGAGAACATTTTCTGGGGGAGTGGTGATGAATGGAATCCAGCACAAGCAGTGGGTGCTTGGGTTGATGAGAAAAAGTATTACAATTATTGGCATAACTCTTGTGTTGATGGACAAATGTGTGGACATTATACACAGGTTGTGTGGGGTGATACAAGGAGAGTTGGATGTGCTAGTGTTGTTTGTTCTGAAGGGAAAGGTACTTTTATGACATGTAACTATGATCCTCCCGGGAATTACTATGGAGAGAAACCTTATTGAAAGGAAATGATTGTTGAAGAAAGAATTGAGACAAACACAGCTCAATAATGTTGTTGTCATCATTTAGGTTTAGAGGCTTATCTTCTTTGTCTGTTGCTGTGTTTGACATGTGAGTGCTATTGATTGTAAATTGTATCTTTTGTTGAATTGTTTAACTCTGTATCTCTGTTTTTATTGCTGGCTTAATGGAGTATAGATAATTATTAATGAAATAAAAANNGTCTGATTTTTTCATT
TARGET=37,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9138
PRIMER_LEFT_PENALTY=0.818184
PRIMER_RIGHT_PENALTY=0.095629
PRIMER_LEFT_SEQUENCE=ACAGTGGGTAGTTCAACGACAG
PRIMER_RIGHT_SEQUENCE=CGAGCAATGTTATGAGGAATCA
PRIMER_LEFT=3,22
PRIMER_RIGHT=202,22
PRIMER_LEFT_TM=59.182
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=200
PRIMER_PAIR_PENALTY_1=0.9473
PRIMER_LEFT_1_PENALTY=0.818184
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=ACAGTGGGTAGTTCAACGACAG
PRIMER_RIGHT_1_SEQUENCE=AATGAGTTAGCTTTTCGTCCCA
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=252,22
PRIMER_LEFT_1_TM=59.182
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=1.1148
PRIMER_LEFT_2_PENALTY=0.818184
PRIMER_RIGHT_2_PENALTY=0.296664
PRIMER_LEFT_2_SEQUENCE=ACAGTGGGTAGTTCAACGACAG
PRIMER_RIGHT_2_SEQUENCE=ATTGCGCGTAATGAGTTAGCTT
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=261,22
PRIMER_LEFT_2_TM=59.182
PRIMER_RIGHT_2_TM=60.297
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=1.2184
PRIMER_LEFT_3_PENALTY=0.818184
PRIMER_RIGHT_3_PENALTY=0.400170
PRIMER_LEFT_3_SEQUENCE=ACAGTGGGTAGTTCAACGACAG
PRIMER_RIGHT_3_SEQUENCE=GCGCGTAATGAGTTAGCTTTTC
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=258,22
PRIMER_LEFT_3_TM=59.182
PRIMER_RIGHT_3_TM=60.400
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=1.2927
PRIMER_LEFT_4_PENALTY=0.818184
PRIMER_RIGHT_4_PENALTY=0.474473
PRIMER_LEFT_4_SEQUENCE=ACAGTGGGTAGTTCAACGACAG
PRIMER_RIGHT_4_SEQUENCE=TACTAGGGGATGTAACCCCACA
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=230,22
PRIMER_LEFT_4_TM=59.182
PRIMER_RIGHT_4_TM=60.474
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_03812 Ps_contig_mira-and-tgicl-ass_franssen_31121
SEQUENCE=TATTTTNATACTTATCTTCTTATTTCTCAATGGTGTGATTATTGAGAAATGAAGGAAGTGTTGGAAACCCTTTGCNCTTGAAATCTGTGAATCATATATCACTCATATGCAGATCAGTGGAAGAATCAATGGATTTTTACCAGAATGTTCTTGGTTTCTTCCCAATTAGAAGGCCTGGTTCTTTTAATTTTGATGGAGCATGGCTATTTGGGTTTGGGATTGGTATTCATTTGTTAGAAGCAGAGAACCCAGAGAAGTTACCAAAGAAAACAGAAATTAATCCAAAGGATAATCATATATCTTTTCAGTGTGAGAGTATGGGAGCAGTTGAGAAAAAGCTGAAAGAAATGGAAATCGATTACGTTAGAGCAATAGTAGAGGAAGGTGGAATTCAAGTAGATCAGTTATTTTTTCATGATCCAGATGGATTCATGATTGAGATATGCAATTGTGACAGCCTTCCTGTGATTCCATTAGCTGGTGAGGTGGCAAGATCATGCTCTCGTTTGAATCTTCAACAACTAATGCAGAATCAGAATCAGCAACAACAACAACAGATACATAAGATTGTTAAGTGATTGGTTATTATAGTATTCTTGTGATTGTGATGTGACCAATTCATGCATCTTCGATGAGTCATGGAATTGTACTTGCTTTTCTTTTGTTGTTGCTTTCTACTTGTCGATTTATGATGTTGTTTTTATTTATTATGTACTTGTTATTAGTAAATAATTGTCAAGTTGTTTGTACTCGAAACAGAGTGGTTTTTTTTAGTTTGTTGTGATTGGTGTTTGGTTTGTTATGGTCTTTTCTTTATTACAAGTCTATTTGTCTAAGGTTTGGTATATAATATTGTCTTTTTTAAG
TARGET=543,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1405
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=0.008216
PRIMER_LEFT_SEQUENCE=TGTGATTCCATTAGCTGGTGAG
PRIMER_RIGHT_SEQUENCE=TGGTCACATCACAATCACAAGA
PRIMER_LEFT=463,22
PRIMER_RIGHT=615,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.3945
PRIMER_LEFT_1_PENALTY=0.386286
PRIMER_RIGHT_1_PENALTY=0.008216
PRIMER_LEFT_1_SEQUENCE=CAAGATCATGCTCTCGTTTGAA
PRIMER_RIGHT_1_SEQUENCE=TGGTCACATCACAATCACAAGA
PRIMER_LEFT_1=489,22
PRIMER_RIGHT_1=615,22
PRIMER_LEFT_1_TM=60.386
PRIMER_RIGHT_1_TM=60.008
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=0.4057
PRIMER_LEFT_2_PENALTY=0.397495
PRIMER_RIGHT_2_PENALTY=0.008216
PRIMER_LEFT_2_SEQUENCE=GAGATATGCAATTGTGACAGCC
PRIMER_RIGHT_2_SEQUENCE=TGGTCACATCACAATCACAAGA
PRIMER_LEFT_2=437,22
PRIMER_RIGHT_2=615,22
PRIMER_LEFT_2_TM=59.603
PRIMER_RIGHT_2_TM=60.008
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.7121
PRIMER_LEFT_3_PENALTY=0.106310
PRIMER_RIGHT_3_PENALTY=0.605838
PRIMER_LEFT_3_SEQUENCE=TCCAGATGGATTCATGATTGAG
PRIMER_RIGHT_3_SEQUENCE=CTCATCGAAGATGCATGAATTG
PRIMER_LEFT_3=418,22
PRIMER_RIGHT_3=635,22
PRIMER_LEFT_3_TM=59.894
PRIMER_RIGHT_3_TM=60.606
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.7381
PRIMER_LEFT_4_PENALTY=0.132262
PRIMER_RIGHT_4_PENALTY=0.605838
PRIMER_LEFT_4_SEQUENCE=CTGTGATTCCATTAGCTGGTGA
PRIMER_RIGHT_4_SEQUENCE=CTCATCGAAGATGCATGAATTG
PRIMER_LEFT_4=462,22
PRIMER_RIGHT_4=635,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=60.606
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=174
=
PRIMER_SEQUENCE_ID=SSR_03813 Ps_contig_mira-and-tgicl-ass_franssen_31127
SEQUENCE=GTTATAAAAAATGACTGATTGAATTTTTAGTTAAATGCACATCTTTCAAATCATACCATTATACCATTGTAATTGTAAAATGAAGTGTCAAGATAAATCATAAAATGCTCATCTTATAAGTTCTGATACTAGGGCTGTAAGACTATCGCGCGACTAGGGAAAATTCTCTATATATGCCCTGACAGTAATCATACAAAATGATTAAGCTCTCCCACTTTGCTTTTTTGTTCTGGAGTCGGTTGTTGAAACCGCTGATCCAGAGAGCTTGTTAACCTCATAGAGCAATACAGCCGAGAACAGAAAACATGTAGTAGTGCATACTCGGTGGACAGTGCTTGCAGCAATCAAAGCAACTACTTCTGCTACTATGACAGCACCCCAGCCAGTTTTTGCACCTGCTGATTCCTGAAAATTTCCAATGAAAGTTAGGAGCACAAGAAATAAGAGAGAGCTGGTGAACCCTCCAAGTATTGTGTATAGTTCAGACGATGCAAGTTTCCCACGGTACATTTCTTGAAGTGAGAGGATGACAATGAATAAAAGAAAGGAATACGTCATGGAACTCCCAGACCCTGCCATGGCCTCACTCACTCAGATCAAGAAGACGCAACTTTCAGTTCAGATCTCTCTATCACACGCGATAGTATTTTTTTAATTAAAAACGCGCCCTGAGCTACACTCTACGGTCCCCGAC
TARGET=583,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5762
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=0.322707
PRIMER_LEFT_SEQUENCE=TCCAATGAAAGTTAGGAGCACA
PRIMER_RIGHT_SEQUENCE=GACCGTAGAGTGTAGCTCAGGG
PRIMER_LEFT=414,22
PRIMER_RIGHT=687,22
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=60.323
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=59.091
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.5762
PRIMER_LEFT_1_PENALTY=0.253482
PRIMER_RIGHT_1_PENALTY=0.322707
PRIMER_LEFT_1_SEQUENCE=TCCAATGAAAGTTAGGAGCACA
PRIMER_RIGHT_1_SEQUENCE=GGACCGTAGAGTGTAGCTCAGG
PRIMER_LEFT_1=414,22
PRIMER_RIGHT_1=688,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=60.323
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=59.091
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.5762
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.322707
PRIMER_LEFT_2_SEQUENCE=TCCAATGAAAGTTAGGAGCACA
PRIMER_RIGHT_2_SEQUENCE=GGGACCGTAGAGTGTAGCTCAG
PRIMER_LEFT_2=414,22
PRIMER_RIGHT_2=689,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.323
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=59.091
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.7982
PRIMER_LEFT_3_PENALTY=0.475463
PRIMER_RIGHT_3_PENALTY=0.322707
PRIMER_LEFT_3_SEQUENCE=TAAGAGAGAGCTGGTGAACCCT
PRIMER_RIGHT_3_SEQUENCE=GGACCGTAGAGTGTAGCTCAGG
PRIMER_LEFT_3=441,22
PRIMER_RIGHT_3=688,22
PRIMER_LEFT_3_TM=59.525
PRIMER_RIGHT_3_TM=60.323
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=59.091
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=248
PRIMER_PAIR_PENALTY_4=0.7982
PRIMER_LEFT_4_PENALTY=0.475463
PRIMER_RIGHT_4_PENALTY=0.322707
PRIMER_LEFT_4_SEQUENCE=TAAGAGAGAGCTGGTGAACCCT
PRIMER_RIGHT_4_SEQUENCE=GGGACCGTAGAGTGTAGCTCAG
PRIMER_LEFT_4=441,22
PRIMER_RIGHT_4=689,22
PRIMER_LEFT_4_TM=59.525
PRIMER_RIGHT_4_TM=60.323
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=59.091
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_03814 Ps_contig_mira-and-tgicl-ass_franssen_31131
SEQUENCE=AGAAACCTAGTATATTTTCNAATCCAACTAAACGTAGAAGTGAAAAGGTTGCGTTGCGTGCAAAGTGGATTGAGATTAAACAAATAACATAACATAACATTGTGTTCTGGTTTATTGAGTTGTGGCAACAAAGCCTAGATCAACTCTATGGCTTCCTTCACCAAATCACTCTTTCTTTTTCTTTTCATTCAATTTACTTTCTCACTTTTTCCCTCATCAATCGCTCAATCCCCTACCCCAGGGTCTTCTGAAGGCTACACTATTTACGGTCGAGTGAAGATCCCTGGTTTTGGAACAAAAGAGTTCAGCCTTCCTGCAAAAGTTTCAAATGTCAAAGTCATACTTAATGGTGGTCAAAGAGTTTCTTTTCTGAGGCCCGATGGATATTTCTCATTCCACAATGTGCCTGCAGGGACTCATTTAATTGAAGTGGCAGCAACAGGCTATTTCTTTTCTCCTGTACGAGTTGACGTTAGTGCCAGAAACCCTGGCAAAATTCAGGCAGCACTGACAGAAAATAGGAGGGGGCTCAGTGAGTTTGTCTTAGAGCCATTGAAGGATGAACAATATTATGAGATTAGGGAACCATTCTCTGTTATGTCCATTGTGAAGAGTCCAATGGGTCTGATGATGGGATTTATGCTTATTGTTGTCTTCCTTATGCCCAAATTGATGGAGAACATGGATCCAGAAGAAATGAAACGCGCCCAAGAAGAAATGAGAAGCCAAGGAGTTCCATCTCTAGCAAACTTGTTACCTGGTGCCGCACGAAGCTAGGGCAATTTAGTTTTTCTGAGCAGAAGCTAAAGATGTATTACGTACCATGATTAGAGAATTTTGTTATGACAAATTGGTGCTGAGAAAAATCTTTGATTATTGAAGTTGTTGAATAGTTGAATTTGAAATTGCTGATAGGGATGTTAGCATGTTTAATATAACCAGACATGAATAGGCTTCCATGTAACTTGGGTCACTTTCTTCAATGTTGATAACCATGCACGGCCAATATGGATCATANGTTTTGGGTTGGGCTTGT
TARGET=84,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1650
PRIMER_LEFT_PENALTY=0.130883
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=CGTGCAAAGTGGATTGAGATTA
PRIMER_RIGHT_SEQUENCE=ATTTGGTGAAGGAAGCCATAGA
PRIMER_LEFT=56,22
PRIMER_RIGHT=165,22
PRIMER_LEFT_TM=60.131
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=0.1650
PRIMER_LEFT_1_PENALTY=0.130883
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=CGTGCAAAGTGGATTGAGATTA
PRIMER_RIGHT_1_SEQUENCE=GATTTGGTGAAGGAAGCCATAG
PRIMER_LEFT_1=56,22
PRIMER_RIGHT_1=166,22
PRIMER_LEFT_1_TM=60.131
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=0.2089
PRIMER_LEFT_2_PENALTY=0.130883
PRIMER_RIGHT_2_PENALTY=0.078029
PRIMER_LEFT_2_SEQUENCE=CGTGCAAAGTGGATTGAGATTA
PRIMER_RIGHT_2_SEQUENCE=CAGGAAGGCTGAACTCTTTTGT
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=315,22
PRIMER_LEFT_2_TM=60.131
PRIMER_RIGHT_2_TM=59.922
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.2612
PRIMER_LEFT_3_PENALTY=0.130883
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=CGTGCAAAGTGGATTGAGATTA
PRIMER_RIGHT_3_SEQUENCE=GGTGAAGGAAGCCATAGAGTTG
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=161,22
PRIMER_LEFT_3_TM=60.131
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.2612
PRIMER_LEFT_4_PENALTY=0.130883
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=CGTGCAAAGTGGATTGAGATTA
PRIMER_RIGHT_4_SEQUENCE=TGGTGAAGGAAGCCATAGAGTT
PRIMER_LEFT_4=56,22
PRIMER_RIGHT_4=162,22
PRIMER_LEFT_4_TM=60.131
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=107
=
PRIMER_SEQUENCE_ID=SSR_03815 Ps_contig_mira-and-tgicl-ass_franssen_31140
SEQUENCE=TTTAATTTTTTTATTAAAACAAAGAACATTAAATTTTGTTTAACCAATGTTCAAAGGTGTCTCATTGGCTTTTCAATAAAGTAAACAATAGATACATCAAATTGTAATAGCATACAATACAATATTTCATATAGTAGTTGTTACATCATTTGGACACTTCCTTATTTGGTGAGCATACAAATTATGAATCACATTTTCATTTCATAACATGTGTAGTTTTGTATAAATTATGTGTCCTTGAATGAACCTATATATATATTTGTGACAGGTTGTTAATTCTATTCAAAGACACGGTTATGACAAGTAGGGTTGCAAGGATAAGAACAATCTATCTGTTGAAAACCTTTTCTCTCAAAATACCAATCTCCCACAGCCTTTGCTATTGTTGTATTGACAAGGAGTGGAGAGTCATTTCTCATCCATGTTTCCTGTATTCCAGTTTGGCAGTGGGAATAGCAACCGTCGATAAACATTCCTTTAGATGGAGAATTACTAACCACACTAATTGCCTTTAAGAATTCTGTCCTGAAACCTTGCATTGTGGTTAATTGATTAGATGAGCAATCTTTTATATCGAGCTTGCAGCTACGCCAAGTGCCATGTGGATCAGCAACACCGGGTGCCAAAATATTCTTAATCTGCCATGAGTCATAGGCTGCATTTACAAAGAAGATTGGTGTACTGATTTGTGCGACCACATTTTGCGGGAAAAAACACAGCCCTGGTGAAAGTCTTGATGTGCAAGATGAAGGCAAATTCTTTGCCGAGGCATGTGTTTGAACGACTTGGCTGTAGAATTGTTCAATGTGTTGTGCTCCAGAGACAGCTTTTACATTGATAAAATAACCAGCATCTGAGACGCATTTAACTTTAGCTGCTGCAGGAAGTAAAGTTCTAAAGCGATCGCATTGTAAAATAGAAGTCAATCCTCCAGCAGAACATCCAGATAGAATTGCATTTTTAGCATTTTTCATTCCTTTTGCTAGTAAATCCTCAATGACAGCAACAAATATTCTTCCTCCTCTATAGTGCAAATTGGTTGCTGGATCAACAGCTTCAACATCACCGGTAAATGATGAACCGTCACAATACCTAACCTTGATTCTGTTCCAATTGTAGAAATCTGGATTAAACTTTTTCCATTACTAAAAAAACCCGAAAAGGAAAGTGTTGTATCCATTTTCTTCGACGATCCTAATGGGGTGTCTCTACGGTCAAGGCAAGTAGAGGCATTGTTACACCATCCTCCTCCCACGAAGTGAACAATCCAGTTATCAATTCCGGCATCAAATCCCTTATCAAAGTGGTAAGCCGGTGGACTCCCATCCAAACAAACAGCTCCTTTTGGTACTGCACTTTGAACATAAGTGATTGGCACAGCAACCCCTTCAGCTGATTTTACTAATAGCAGTGTGCATATTACAAGACTTAACAAATGCTCCATTTTTGTACTCTTCATTATTTAGGTGGTTGAAGGTTGAATGGAAGAAGAAGAAGAAGAATGAATGAATCNCNAAATTGAAACTGAGTTATGGCTTATAAATGCTTTCAGCTACCGACAGTAGTTACTGCTAGCAGAGCAAAACCGAGTGAAGTACCCCGAC
TARGET=249,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3611
PRIMER_LEFT_PENALTY=0.360320
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=GGTGTCTCATTGGCTTTTCAAT
PRIMER_RIGHT_SEQUENCE=TTGTTCTTATCCTTGCAACCCT
PRIMER_LEFT=55,22
PRIMER_RIGHT=326,22
PRIMER_LEFT_TM=60.360
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.3642
PRIMER_LEFT_1_PENALTY=0.363436
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=AAAGGTGTCTCATTGGCTTTTC
PRIMER_RIGHT_1_SEQUENCE=TTGTTCTTATCCTTGCAACCCT
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=326,22
PRIMER_LEFT_1_TM=59.637
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.5044
PRIMER_LEFT_2_PENALTY=0.503696
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=ACCAATGTTCAAAGGTGTCTCA
PRIMER_RIGHT_2_SEQUENCE=TTGTTCTTATCCTTGCAACCCT
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=326,22
PRIMER_LEFT_2_TM=59.496
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.5146
PRIMER_LEFT_3_PENALTY=0.513831
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=CAAAGGTGTCTCATTGGCTTTT
PRIMER_RIGHT_3_SEQUENCE=TTGTTCTTATCCTTGCAACCCT
PRIMER_LEFT_3=51,22
PRIMER_RIGHT_3=326,22
PRIMER_LEFT_3_TM=60.514
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.5299
PRIMER_LEFT_4_PENALTY=0.529160
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=TGGACACTTCCTTATTTGGTGA
PRIMER_RIGHT_4_SEQUENCE=TTGTTCTTATCCTTGCAACCCT
PRIMER_LEFT_4=150,22
PRIMER_RIGHT_4=326,22
PRIMER_LEFT_4_TM=59.471
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_03816 Ps_contig_mira-and-tgicl-ass_franssen_31140
SEQUENCE=TTTAATTTTTTTATTAAAACAAAGAACATTAAATTTTGTTTAACCAATGTTCAAAGGTGTCTCATTGGCTTTTCAATAAAGTAAACAATAGATACATCAAATTGTAATAGCATACAATACAATATTTCATATAGTAGTTGTTACATCATTTGGACACTTCCTTATTTGGTGAGCATACAAATTATGAATCACATTTTCATTTCATAACATGTGTAGTTTTGTATAAATTATGTGTCCTTGAATGAACCTATATATATATTTGTGACAGGTTGTTAATTCTATTCAAAGACACGGTTATGACAAGTAGGGTTGCAAGGATAAGAACAATCTATCTGTTGAAAACCTTTTCTCTCAAAATACCAATCTCCCACAGCCTTTGCTATTGTTGTATTGACAAGGAGTGGAGAGTCATTTCTCATCCATGTTTCCTGTATTCCAGTTTGGCAGTGGGAATAGCAACCGTCGATAAACATTCCTTTAGATGGAGAATTACTAACCACACTAATTGCCTTTAAGAATTCTGTCCTGAAACCTTGCATTGTGGTTAATTGATTAGATGAGCAATCTTTTATATCGAGCTTGCAGCTACGCCAAGTGCCATGTGGATCAGCAACACCGGGTGCCAAAATATTCTTAATCTGCCATGAGTCATAGGCTGCATTTACAAAGAAGATTGGTGTACTGATTTGTGCGACCACATTTTGCGGGAAAAAACACAGCCCTGGTGAAAGTCTTGATGTGCAAGATGAAGGCAAATTCTTTGCCGAGGCATGTGTTTGAACGACTTGGCTGTAGAATTGTTCAATGTGTTGTGCTCCAGAGACAGCTTTTACATTGATAAAATAACCAGCATCTGAGACGCATTTAACTTTAGCTGCTGCAGGAAGTAAAGTTCTAAAGCGATCGCATTGTAAAATAGAAGTCAATCCTCCAGCAGAACATCCAGATAGAATTGCATTTTTAGCATTTTTCATTCCTTTTGCTAGTAAATCCTCAATGACAGCAACAAATATTCTTCCTCCTCTATAGTGCAAATTGGTTGCTGGATCAACAGCTTCAACATCACCGGTAAATGATGAACCGTCACAATACCTAACCTTGATTCTGTTCCAATTGTAGAAATCTGGATTAAACTTTTTCCATTACTAAAAAAACCCGAAAAGGAAAGTGTTGTATCCATTTTCTTCGACGATCCTAATGGGGTGTCTCTACGGTCAAGGCAAGTAGAGGCATTGTTACACCATCCTCCTCCCACGAAGTGAACAATCCAGTTATCAATTCCGGCATCAAATCCCTTATCAAAGTGGTAAGCCGGTGGACTCCCATCCAAACAAACAGCTCCTTTTGGTACTGCACTTTGAACATAAGTGATTGGCACAGCAACCCCTTCAGCTGATTTTACTAATAGCAGTGTGCATATTACAAGACTTAACAAATGCTCCATTTTTGTACTCTTCATTATTTAGGTGGTTGAAGGTTGAATGGAAGAAGAAGAAGAAGAATGAATGAATCNCNAAATTGAAACTGAGTTATGGCTTATAAATGCTTTCAGCTACCGACAGTAGTTACTGCTAGCAGAGCAAAACCGAGTGAAGTACCCCGAC
TARGET=1485,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1725
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.171757
PRIMER_LEFT_SEQUENCE=CAACCCCTTCAGCTGATTTTAC
PRIMER_RIGHT_SEQUENCE=GGTACTTCACTCGGTTTTGCTC
PRIMER_LEFT=1380,22
PRIMER_RIGHT=1598,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.172
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.3464
PRIMER_LEFT_1_PENALTY=0.174657
PRIMER_RIGHT_1_PENALTY=0.171757
PRIMER_LEFT_1_SEQUENCE=GCTCCTTTTGGTACTGCACTTT
PRIMER_RIGHT_1_SEQUENCE=GGTACTTCACTCGGTTTTGCTC
PRIMER_LEFT_1=1337,22
PRIMER_RIGHT_1=1598,22
PRIMER_LEFT_1_TM=59.825
PRIMER_RIGHT_1_TM=60.172
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.3464
PRIMER_LEFT_2_PENALTY=0.174657
PRIMER_RIGHT_2_PENALTY=0.171757
PRIMER_LEFT_2_SEQUENCE=AAACAGCTCCTTTTGGTACTGC
PRIMER_RIGHT_2_SEQUENCE=GGTACTTCACTCGGTTTTGCTC
PRIMER_LEFT_2=1332,22
PRIMER_RIGHT_2=1598,22
PRIMER_LEFT_2_TM=59.825
PRIMER_RIGHT_2_TM=60.172
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=0.3870
PRIMER_LEFT_3_PENALTY=0.215215
PRIMER_RIGHT_3_PENALTY=0.171757
PRIMER_LEFT_3_SEQUENCE=TCCTTTTGGTACTGCACTTTGA
PRIMER_RIGHT_3_SEQUENCE=GGTACTTCACTCGGTTTTGCTC
PRIMER_LEFT_3=1339,22
PRIMER_RIGHT_3=1598,22
PRIMER_LEFT_3_TM=59.785
PRIMER_RIGHT_3_TM=60.172
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.4436
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.442900
PRIMER_LEFT_4_SEQUENCE=CAACCCCTTCAGCTGATTTTAC
PRIMER_RIGHT_4_SEQUENCE=TACTTCACTCGGTTTTGCTCTG
PRIMER_LEFT_4=1380,22
PRIMER_RIGHT_4=1596,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.557
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_03817 Ps_contig_mira-and-tgicl-ass_franssen_31140
SEQUENCE=TTTAATTTTTTTATTAAAACAAAGAACATTAAATTTTGTTTAACCAATGTTCAAAGGTGTCTCATTGGCTTTTCAATAAAGTAAACAATAGATACATCAAATTGTAATAGCATACAATACAATATTTCATATAGTAGTTGTTACATCATTTGGACACTTCCTTATTTGGTGAGCATACAAATTATGAATCACATTTTCATTTCATAACATGTGTAGTTTTGTATAAATTATGTGTCCTTGAATGAACCTATATATATATTTGTGACAGGTTGTTAATTCTATTCAAAGACACGGTTATGACAAGTAGGGTTGCAAGGATAAGAACAATCTATCTGTTGAAAACCTTTTCTCTCAAAATACCAATCTCCCACAGCCTTTGCTATTGTTGTATTGACAAGGAGTGGAGAGTCATTTCTCATCCATGTTTCCTGTATTCCAGTTTGGCAGTGGGAATAGCAACCGTCGATAAACATTCCTTTAGATGGAGAATTACTAACCACACTAATTGCCTTTAAGAATTCTGTCCTGAAACCTTGCATTGTGGTTAATTGATTAGATGAGCAATCTTTTATATCGAGCTTGCAGCTACGCCAAGTGCCATGTGGATCAGCAACACCGGGTGCCAAAATATTCTTAATCTGCCATGAGTCATAGGCTGCATTTACAAAGAAGATTGGTGTACTGATTTGTGCGACCACATTTTGCGGGAAAAAACACAGCCCTGGTGAAAGTCTTGATGTGCAAGATGAAGGCAAATTCTTTGCCGAGGCATGTGTTTGAACGACTTGGCTGTAGAATTGTTCAATGTGTTGTGCTCCAGAGACAGCTTTTACATTGATAAAATAACCAGCATCTGAGACGCATTTAACTTTAGCTGCTGCAGGAAGTAAAGTTCTAAAGCGATCGCATTGTAAAATAGAAGTCAATCCTCCAGCAGAACATCCAGATAGAATTGCATTTTTAGCATTTTTCATTCCTTTTGCTAGTAAATCCTCAATGACAGCAACAAATATTCTTCCTCCTCTATAGTGCAAATTGGTTGCTGGATCAACAGCTTCAACATCACCGGTAAATGATGAACCGTCACAATACCTAACCTTGATTCTGTTCCAATTGTAGAAATCTGGATTAAACTTTTTCCATTACTAAAAAAACCCGAAAAGGAAAGTGTTGTATCCATTTTCTTCGACGATCCTAATGGGGTGTCTCTACGGTCAAGGCAAGTAGAGGCATTGTTACACCATCCTCCTCCCACGAAGTGAACAATCCAGTTATCAATTCCGGCATCAAATCCCTTATCAAAGTGGTAAGCCGGTGGACTCCCATCCAAACAAACAGCTCCTTTTGGTACTGCACTTTGAACATAAGTGATTGGCACAGCAACCCCTTCAGCTGATTTTACTAATAGCAGTGTGCATATTACAAGACTTAACAAATGCTCCATTTTTGTACTCTTCATTATTTAGGTGGTTGAAGGTTGAATGGAAGAAGAAGAAGAAGAATGAATGAATCNCNAAATTGAAACTGAGTTATGGCTTATAAATGCTTTCAGCTACCGACAGTAGTTACTGCTAGCAGAGCAAAACCGAGTGAAGTACCCCGAC
TARGET=1500,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1725
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.171757
PRIMER_LEFT_SEQUENCE=CAACCCCTTCAGCTGATTTTAC
PRIMER_RIGHT_SEQUENCE=GGTACTTCACTCGGTTTTGCTC
PRIMER_LEFT=1380,22
PRIMER_RIGHT=1598,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.172
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.3464
PRIMER_LEFT_1_PENALTY=0.174657
PRIMER_RIGHT_1_PENALTY=0.171757
PRIMER_LEFT_1_SEQUENCE=GCTCCTTTTGGTACTGCACTTT
PRIMER_RIGHT_1_SEQUENCE=GGTACTTCACTCGGTTTTGCTC
PRIMER_LEFT_1=1337,22
PRIMER_RIGHT_1=1598,22
PRIMER_LEFT_1_TM=59.825
PRIMER_RIGHT_1_TM=60.172
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.3464
PRIMER_LEFT_2_PENALTY=0.174657
PRIMER_RIGHT_2_PENALTY=0.171757
PRIMER_LEFT_2_SEQUENCE=AAACAGCTCCTTTTGGTACTGC
PRIMER_RIGHT_2_SEQUENCE=GGTACTTCACTCGGTTTTGCTC
PRIMER_LEFT_2=1332,22
PRIMER_RIGHT_2=1598,22
PRIMER_LEFT_2_TM=59.825
PRIMER_RIGHT_2_TM=60.172
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=0.3870
PRIMER_LEFT_3_PENALTY=0.215215
PRIMER_RIGHT_3_PENALTY=0.171757
PRIMER_LEFT_3_SEQUENCE=TCCTTTTGGTACTGCACTTTGA
PRIMER_RIGHT_3_SEQUENCE=GGTACTTCACTCGGTTTTGCTC
PRIMER_LEFT_3=1339,22
PRIMER_RIGHT_3=1598,22
PRIMER_LEFT_3_TM=59.785
PRIMER_RIGHT_3_TM=60.172
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.4436
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.442900
PRIMER_LEFT_4_SEQUENCE=CAACCCCTTCAGCTGATTTTAC
PRIMER_RIGHT_4_SEQUENCE=TACTTCACTCGGTTTTGCTCTG
PRIMER_LEFT_4=1380,22
PRIMER_RIGHT_4=1596,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.557
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_03818 Ps_contig_mira-and-tgicl-ass_franssen_31141
SEQUENCE=CGCAAAAGATTATAATATTGAAGTGAAAACATATGTTATAGTGTAAAACAATGATCCTTTTTTTTCACAAGACATTGCTAAGTGACACCATTGGTTTCAAAAACAATGTCAAACAACCTCTGGTGTTTCTTCTAAAAAACTGCTTTCTTGCTGCATTCTTAAGTGTACCCACAAAAACTTCAATGGATTGAGACATGTGTAACTCTTTACTGCTATACAAACAATGTTGAAAAGAACTACAGTACACTGGCACAGGGGACTCAGACAAGAGAGTGTGCTACAGAAAGCAAATGAGTGAAAAGAAAGACTAAGAATGATTGATCACTGACTCTCGATCAATTACATATGGTGAATAACTGAATATATGGAGAATTTGTCGCCACGTTCGACCAAACACAACCTCTAATATTGTGTATGTGTGTGTATTTACTTTCCAATGCCAAGTTCTTTTGTGATGTCTCTTGTCAAGCTAAATTGCAAAGTGTTGTGCCATGGAAATAAGGTGCGGGTGACTGGAAGCTTCCTCCGAAGATCCTTGAAAGTAGAATCGGGAAGGTTTAGATATGAAGCCTCAAGAGAAGCAAGGTATTCTGTTTCATGGTGACGAATAATGCTTGATATGGCAAGCGCACAATCCTCAGGGGCCTGAAAAAGAGTTGCGTCTTTGCATTCATGTTTTGTATCTAACTGCACATTTCCCTCTTCAAAATAATGGGCACCAACCTGCAGTTTCCCTTTTACTTCCACTGTTTGTTGTTCATCTTTGAAATCAATATTCCAAACTGAACACCAATTCCCATTGCAGAAATTCTGTGGGCTATGCCTAGAAGCAGAAATCACTATGGCAAGCTCAAAATCAGAACCCGGTCCCTCTGAATCCTTTCCATTTACACAGTAGACAGAGCAGACACCTTTTGGATAAACTTCTTCCACATATTTAAGTATCTCGGCATCAAGACTGAATCGAAATTCTTCAATGTATGCAGATGCAAGTTCTTCATCCAATGCAGGTCTCACATCCTCACAAACTTGTTTAACATGGTCAACTATTGCAACTTGAGCAGTCCTAGGATCAAGAAACGCATTATCCTCAAGCTCACCAAAGGAAGTAACCAGTACGTCTCCGCTTCGGTCAGGCATTGGAAGAACAATGAAGTGTGATTTGTTGTAAAGAGGAAACGCTTCGGAAGCAGCTTCGTTGAACAAACCATCGTCGTTGAGAATGGATTTCACATCTTTGGCAACGTATTGAATTTCTCCGGGAGGAGAGTTGAGAAGAAACCATTTCGCGATTTCAACTTTTTGCTTCTCGCTGAGTTCAGATTCTTCTTCTTCTTCTGCCATTAACTCACTCTCTTAGAGGAAATGGAAAAACACAGGGCTATTTTTTATTTTATTTTATTATTTTCGGGAAAAAAATCAAAGTCTGAATGGAACTGAAGAATGAAGATCCTCCCCTACTCTGTCG
TARGET=1324,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3980
PRIMER_LEFT_PENALTY=0.054021
PRIMER_RIGHT_PENALTY=0.343977
PRIMER_LEFT_SEQUENCE=GGAAGTAACCAGTACGTCTCCG
PRIMER_RIGHT_SEQUENCE=TAGCCCTGTGTTTTTCCATTTC
PRIMER_LEFT=1104,22
PRIMER_RIGHT=1383,22
PRIMER_LEFT_TM=60.054
PRIMER_RIGHT_TM=60.344
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.5363
PRIMER_LEFT_1_PENALTY=0.192310
PRIMER_RIGHT_1_PENALTY=0.343977
PRIMER_LEFT_1_SEQUENCE=GTAACCAGTACGTCTCCGCTTC
PRIMER_RIGHT_1_SEQUENCE=TAGCCCTGTGTTTTTCCATTTC
PRIMER_LEFT_1=1108,22
PRIMER_RIGHT_1=1383,22
PRIMER_LEFT_1_TM=60.192
PRIMER_RIGHT_1_TM=60.344
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.5363
PRIMER_LEFT_2_PENALTY=0.192310
PRIMER_RIGHT_2_PENALTY=0.343977
PRIMER_LEFT_2_SEQUENCE=GAAGTAACCAGTACGTCTCCGC
PRIMER_RIGHT_2_SEQUENCE=TAGCCCTGTGTTTTTCCATTTC
PRIMER_LEFT_2=1105,22
PRIMER_RIGHT_2=1383,22
PRIMER_LEFT_2_TM=60.192
PRIMER_RIGHT_2_TM=60.344
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.5546
PRIMER_LEFT_3_PENALTY=0.054021
PRIMER_RIGHT_3_PENALTY=0.500551
PRIMER_LEFT_3_SEQUENCE=GGAAGTAACCAGTACGTCTCCG
PRIMER_RIGHT_3_SEQUENCE=CCTGTGTTTTTCCATTTCCTCT
PRIMER_LEFT_3=1104,22
PRIMER_RIGHT_3=1379,22
PRIMER_LEFT_3_TM=60.054
PRIMER_RIGHT_3_TM=59.499
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.5760
PRIMER_LEFT_4_PENALTY=0.232060
PRIMER_RIGHT_4_PENALTY=0.343977
PRIMER_LEFT_4_SEQUENCE=AGTTGAGAAGAAACCATTTCGC
PRIMER_RIGHT_4_SEQUENCE=TAGCCCTGTGTTTTTCCATTTC
PRIMER_LEFT_4=1268,22
PRIMER_RIGHT_4=1383,22
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=60.344
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=116
=
PRIMER_SEQUENCE_ID=SSR_03819 Ps_contig_mira-and-tgicl-ass_franssen_31141
SEQUENCE=CGCAAAAGATTATAATATTGAAGTGAAAACATATGTTATAGTGTAAAACAATGATCCTTTTTTTTCACAAGACATTGCTAAGTGACACCATTGGTTTCAAAAACAATGTCAAACAACCTCTGGTGTTTCTTCTAAAAAACTGCTTTCTTGCTGCATTCTTAAGTGTACCCACAAAAACTTCAATGGATTGAGACATGTGTAACTCTTTACTGCTATACAAACAATGTTGAAAAGAACTACAGTACACTGGCACAGGGGACTCAGACAAGAGAGTGTGCTACAGAAAGCAAATGAGTGAAAAGAAAGACTAAGAATGATTGATCACTGACTCTCGATCAATTACATATGGTGAATAACTGAATATATGGAGAATTTGTCGCCACGTTCGACCAAACACAACCTCTAATATTGTGTATGTGTGTGTATTTACTTTCCAATGCCAAGTTCTTTTGTGATGTCTCTTGTCAAGCTAAATTGCAAAGTGTTGTGCCATGGAAATAAGGTGCGGGTGACTGGAAGCTTCCTCCGAAGATCCTTGAAAGTAGAATCGGGAAGGTTTAGATATGAAGCCTCAAGAGAAGCAAGGTATTCTGTTTCATGGTGACGAATAATGCTTGATATGGCAAGCGCACAATCCTCAGGGGCCTGAAAAAGAGTTGCGTCTTTGCATTCATGTTTTGTATCTAACTGCACATTTCCCTCTTCAAAATAATGGGCACCAACCTGCAGTTTCCCTTTTACTTCCACTGTTTGTTGTTCATCTTTGAAATCAATATTCCAAACTGAACACCAATTCCCATTGCAGAAATTCTGTGGGCTATGCCTAGAAGCAGAAATCACTATGGCAAGCTCAAAATCAGAACCCGGTCCCTCTGAATCCTTTCCATTTACACAGTAGACAGAGCAGACACCTTTTGGATAAACTTCTTCCACATATTTAAGTATCTCGGCATCAAGACTGAATCGAAATTCTTCAATGTATGCAGATGCAAGTTCTTCATCCAATGCAGGTCTCACATCCTCACAAACTTGTTTAACATGGTCAACTATTGCAACTTGAGCAGTCCTAGGATCAAGAAACGCATTATCCTCAAGCTCACCAAAGGAAGTAACCAGTACGTCTCCGCTTCGGTCAGGCATTGGAAGAACAATGAAGTGTGATTTGTTGTAAAGAGGAAACGCTTCGGAAGCAGCTTCGTTGAACAAACCATCGTCGTTGAGAATGGATTTCACATCTTTGGCAACGTATTGAATTTCTCCGGGAGGAGAGTTGAGAAGAAACCATTTCGCGATTTCAACTTTTTGCTTCTCGCTGAGTTCAGATTCTTCTTCTTCTTCTGCCATTAACTCACTCTCTTAGAGGAAATGGAAAAACACAGGGCTATTTTTTATTTTATTTTATTATTTTCGGGAAAAAAATCAAAGTCTGAATGGAACTGAAGAATGAAGATCCTCCCCTACTCTGTCG
TARGET=1387,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2575
PRIMER_LEFT_PENALTY=0.232060
PRIMER_RIGHT_PENALTY=1.025403
PRIMER_LEFT_SEQUENCE=AGTTGAGAAGAAACCATTTCGC
PRIMER_RIGHT_SEQUENCE=CAGAGTAGGGGAGGATCTTCATT
PRIMER_LEFT=1268,22
PRIMER_RIGHT=1464,23
PRIMER_LEFT_TM=59.768
PRIMER_RIGHT_TM=59.975
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=1.2716
PRIMER_LEFT_1_PENALTY=0.232060
PRIMER_RIGHT_1_PENALTY=1.039590
PRIMER_LEFT_1_SEQUENCE=AGTTGAGAAGAAACCATTTCGC
PRIMER_RIGHT_1_SEQUENCE=GGGAGGATCTTCATTCTTCAGTT
PRIMER_LEFT_1=1268,22
PRIMER_RIGHT_1=1456,23
PRIMER_LEFT_1_TM=59.768
PRIMER_RIGHT_1_TM=59.960
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=1.3086
PRIMER_LEFT_2_PENALTY=0.283237
PRIMER_RIGHT_2_PENALTY=1.025403
PRIMER_LEFT_2_SEQUENCE=ACTTTTTGCTTCTCGCTGAGTT
PRIMER_RIGHT_2_SEQUENCE=CAGAGTAGGGGAGGATCTTCATT
PRIMER_LEFT_2=1297,22
PRIMER_RIGHT_2=1464,23
PRIMER_LEFT_2_TM=59.717
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=1.3086
PRIMER_LEFT_3_PENALTY=0.283237
PRIMER_RIGHT_3_PENALTY=1.025403
PRIMER_LEFT_3_SEQUENCE=AACTTTTTGCTTCTCGCTGAGT
PRIMER_RIGHT_3_SEQUENCE=CAGAGTAGGGGAGGATCTTCATT
PRIMER_LEFT_3=1296,22
PRIMER_RIGHT_3=1464,23
PRIMER_LEFT_3_TM=59.717
PRIMER_RIGHT_3_TM=59.975
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=1.3228
PRIMER_LEFT_4_PENALTY=0.283237
PRIMER_RIGHT_4_PENALTY=1.039590
PRIMER_LEFT_4_SEQUENCE=ACTTTTTGCTTCTCGCTGAGTT
PRIMER_RIGHT_4_SEQUENCE=GGGAGGATCTTCATTCTTCAGTT
PRIMER_LEFT_4=1297,22
PRIMER_RIGHT_4=1456,23
PRIMER_LEFT_4_TM=59.717
PRIMER_RIGHT_4_TM=59.960
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_03820 Ps_contig_mira-and-tgicl-ass_franssen_31142
SEQUENCE=TACGTCGGGGGTAACNACGCGCGCACCGTGTATTTGTTTCCCATTCATTCATAATCATAGGAAACTCAAACTGGTAACGATTCACACTCACAATCAGAGAGAAGAGAAAATCACACACACACACACTCTCTCTCTCTGAACCCTTCGTTCTGCAAATATGAGCTCAATTGTTCAGGGTTTTACAAAGTCACTTGCCATGACCGTTCTCTCCGAGATCGGTGACAAGACTTTCTTTGCAGCTGCGATACTGGCTATGCGCCATCCGCGACGCCTTGTCTTAACAGGTTGCCTTGCGGCTTTGATAGTGATGACAATTCTCTCTGTTCTTGTTGGTTGGGCTGCTCCAAATCTGATCTCGCGTAATTGGACTCATCATATTACAACATTCTTGTTCTTGGGATTTGGACTTTGGTCCTTGAAAGAGGCTATATTTGAAGGAGGGGAATCAGAGGAGTTGGCTGAAGTTGAAGCTCAATTGGACAAAGATTGGAAGGCTAAAAATGGAGCTACAAAAGATAGCAAAAAGGATGATGATGCCACAAAAAAGCATAAACAGTCATTTTTATCACAGTTTTTCTCTCCCATCTTGTTACAGGCATTTTCTATCACTTTTTTTGGTGAATGGGGTGACAAGAGCCAGTTGGCTACCATAGGTTTAGCTGCAGATGAAAACCCATTTGGTGTGGTTCTTGGAGGGATTCTGGGGCAAGCATTGTGTACTACTGCTGCTGTTATTGGAGGGAAGAGTTTAGCTTCTCAGATATCTGAAAAAGTGGTTGGACTCTCAGGTGGAATTCTTTTCATTGTTTTTGGCATCCAATCATTCCTTTCTCCAGTTGAATCATGAAGTCTATGCCATTGGTTAGTGATCATTCTAGGCGCTTTTCTTACAAGATATGTTTAGCAAGTATGGTTGTTATAGTGGTTGAGATTGGCTTCCAAAAGAGCTCGCCTTTCCACCTTGGGTCAAAGTTTTCATTCTTTTTAGTTGTTAACTTTTTAAAGATTCTATGGTTCAAAATAAAGACAGCCTTGTAGAACCAGACCCAAATTTAGTATAGGAACTTGATTGGATTATTCCTTTTCAACACGAACTTGCAGGAACAGATTCTCTCTCGGGAGAATTTCACAAATTTGTAAATGTCTGTGAAGAGTCTTTTCACATGACAATGTTGTGTGAAATTTGTCATGTGAAAATTAACATTAAATGATCCATTTCCATGTGCCAACACCTTAACCTCTTTATCTTCCTTTCATAATCTATTATTGCATGCATGTGTGAATTTCCAATGCTTGTTGCTGGTGGAATAAATGATCATTTTAGG
TARGET=112,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0190
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=ACCGTGTATTTGTTTCCCATTC
PRIMER_RIGHT_SEQUENCE=GAACGAAGGGTTCAGAGAGAGA
PRIMER_LEFT=24,22
PRIMER_RIGHT=149,22
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=126
PRIMER_PAIR_PENALTY_1=0.0507
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=ACCGTGTATTTGTTTCCCATTC
PRIMER_RIGHT_1_SEQUENCE=GGTCATGGCAAGTGACTTTGTA
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=201,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.1004
PRIMER_LEFT_2_PENALTY=0.013401
PRIMER_RIGHT_2_PENALTY=0.087031
PRIMER_LEFT_2_SEQUENCE=ACCGTGTATTTGTTTCCCATTC
PRIMER_RIGHT_2_SEQUENCE=GGCAAGTGACTTTGTAAAACCC
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=195,22
PRIMER_LEFT_2_TM=59.987
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=0.3232
PRIMER_LEFT_3_PENALTY=0.013401
PRIMER_RIGHT_3_PENALTY=0.309780
PRIMER_LEFT_3_SEQUENCE=ACCGTGTATTTGTTTCCCATTC
PRIMER_RIGHT_3_SEQUENCE=AAGGCAACCTGTTAAGACAAGG
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=291,22
PRIMER_LEFT_3_TM=59.987
PRIMER_RIGHT_3_TM=59.690
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.3769
PRIMER_LEFT_4_PENALTY=0.013401
PRIMER_RIGHT_4_PENALTY=0.363508
PRIMER_LEFT_4_SEQUENCE=ACCGTGTATTTGTTTCCCATTC
PRIMER_RIGHT_4_SEQUENCE=CATATTTGCAGAACGAAGGGTT
PRIMER_LEFT_4=24,22
PRIMER_RIGHT_4=159,22
PRIMER_LEFT_4_TM=59.987
PRIMER_RIGHT_4_TM=60.364
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=136
=
PRIMER_SEQUENCE_ID=SSR_03821 Ps_contig_mira-and-tgicl-ass_franssen_31142
SEQUENCE=TACGTCGGGGGTAACNACGCGCGCACCGTGTATTTGTTTCCCATTCATTCATAATCATAGGAAACTCAAACTGGTAACGATTCACACTCACAATCAGAGAGAAGAGAAAATCACACACACACACACTCTCTCTCTCTGAACCCTTCGTTCTGCAAATATGAGCTCAATTGTTCAGGGTTTTACAAAGTCACTTGCCATGACCGTTCTCTCCGAGATCGGTGACAAGACTTTCTTTGCAGCTGCGATACTGGCTATGCGCCATCCGCGACGCCTTGTCTTAACAGGTTGCCTTGCGGCTTTGATAGTGATGACAATTCTCTCTGTTCTTGTTGGTTGGGCTGCTCCAAATCTGATCTCGCGTAATTGGACTCATCATATTACAACATTCTTGTTCTTGGGATTTGGACTTTGGTCCTTGAAAGAGGCTATATTTGAAGGAGGGGAATCAGAGGAGTTGGCTGAAGTTGAAGCTCAATTGGACAAAGATTGGAAGGCTAAAAATGGAGCTACAAAAGATAGCAAAAAGGATGATGATGCCACAAAAAAGCATAAACAGTCATTTTTATCACAGTTTTTCTCTCCCATCTTGTTACAGGCATTTTCTATCACTTTTTTTGGTGAATGGGGTGACAAGAGCCAGTTGGCTACCATAGGTTTAGCTGCAGATGAAAACCCATTTGGTGTGGTTCTTGGAGGGATTCTGGGGCAAGCATTGTGTACTACTGCTGCTGTTATTGGAGGGAAGAGTTTAGCTTCTCAGATATCTGAAAAAGTGGTTGGACTCTCAGGTGGAATTCTTTTCATTGTTTTTGGCATCCAATCATTCCTTTCTCCAGTTGAATCATGAAGTCTATGCCATTGGTTAGTGATCATTCTAGGCGCTTTTCTTACAAGATATGTTTAGCAAGTATGGTTGTTATAGTGGTTGAGATTGGCTTCCAAAAGAGCTCGCCTTTCCACCTTGGGTCAAAGTTTTCATTCTTTTTAGTTGTTAACTTTTTAAAGATTCTATGGTTCAAAATAAAGACAGCCTTGTAGAACCAGACCCAAATTTAGTATAGGAACTTGATTGGATTATTCCTTTTCAACACGAACTTGCAGGAACAGATTCTCTCTCGGGAGAATTTCACAAATTTGTAAATGTCTGTGAAGAGTCTTTTCACATGACAATGTTGTGTGAAATTTGTCATGTGAAAATTAACATTAAATGATCCATTTCCATGTGCCAACACCTTAACCTCTTTATCTTCCTTTCATAATCTATTATTGCATGCATGTGTGAATTTCCAATGCTTGTTGCTGGTGGAATAAATGATCATTTTAGG
TARGET=126,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0507
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=ACCGTGTATTTGTTTCCCATTC
PRIMER_RIGHT_SEQUENCE=GGTCATGGCAAGTGACTTTGTA
PRIMER_LEFT=24,22
PRIMER_RIGHT=201,22
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.1004
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=ACCGTGTATTTGTTTCCCATTC
PRIMER_RIGHT_1_SEQUENCE=GGCAAGTGACTTTGTAAAACCC
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=195,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.3232
PRIMER_LEFT_2_PENALTY=0.013401
PRIMER_RIGHT_2_PENALTY=0.309780
PRIMER_LEFT_2_SEQUENCE=ACCGTGTATTTGTTTCCCATTC
PRIMER_RIGHT_2_SEQUENCE=AAGGCAACCTGTTAAGACAAGG
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=291,22
PRIMER_LEFT_2_TM=59.987
PRIMER_RIGHT_2_TM=59.690
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.3769
PRIMER_LEFT_3_PENALTY=0.013401
PRIMER_RIGHT_3_PENALTY=0.363508
PRIMER_LEFT_3_SEQUENCE=ACCGTGTATTTGTTTCCCATTC
PRIMER_RIGHT_3_SEQUENCE=CATATTTGCAGAACGAAGGGTT
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=159,22
PRIMER_LEFT_3_TM=59.987
PRIMER_RIGHT_3_TM=60.364
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=0.4101
PRIMER_LEFT_4_PENALTY=0.013401
PRIMER_RIGHT_4_PENALTY=0.396675
PRIMER_LEFT_4_SEQUENCE=ACCGTGTATTTGTTTCCCATTC
PRIMER_RIGHT_4_SEQUENCE=TTGAGCTCATATTTGCAGAACG
PRIMER_LEFT_4=24,22
PRIMER_RIGHT_4=166,22
PRIMER_LEFT_4_TM=59.987
PRIMER_RIGHT_4_TM=60.397
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_03822 Ps_contig_mira-and-tgicl-ass_franssen_31155
SEQUENCE=TTTTNTNAAAAAAATAATTTCTCTATTATAAATTTAATATAATCTTTATTGTAAAATTACACATACAGTATAAAATAGTTGTATACTAAGATACAACAAAAGCTAGAATTTTATAATAAAGGACATAATAGTAACATTAAATTATAGGAATTTTCTCACAGAAGAACATCACGAAACATCTTCTCAAGATTCATAAACGATGATCCATTTGGACCAGAAGCAGCGTTTTGTGCTAATTTCTTCCATTCCAAAGCTTTTTCTTTCATCTCTTTACCTTTTTCCCCCTCCACCATCTCCTTCACAAGACTCTCAATCTTATCCCTCTTAGCATCTTCAATCTCCAAACCAATTCCCCATTCATGGCAACAAAATCTACAATTAGTTTGTTGTTCAGCAAAGAAAGGCCAACATATCATTGGAACACCGCCGCACACACTTTCCAACGTTGAGTTCCAACCACTATGTGTCAAAAAACCTCCAATGGCTGAATGATCCAAAACCTCCTCTTGTGGACACCAACTTGATAACATTCCTCTATTTTTTGTCTCTTCCAGAAACTCTTGAGGAAGAACTGAGTTTTCACCGGCTACAAGATCAGGCCTTATTACCCATAAAAATGGTATTTTACTATTTGCTAATCCCCAAGCAAACTCAATCATCTGTTCATTTGTCATCACTGTGATACTTCCAAAATTCACATAAACAACACTATTTGGTTCCTTGCTATTCAGCCATTCTAAACACTCTGGCTCCTCTTTCCAAAGATTAGAACCAAATGAATTCAGTTCTTTATCAGTTACTTCTTTCAAGAGTAAATGCAAGGGACCAATGGAATAAACAGGAGGAAAATTCAACGAAGAGAATGCTTCCAAAACATTATGTTCCAAATCATCAAAAGTGTTAAGAATAATTGCGGAGGCTTTTTGAGCTCTCTCGCACTCACCTAATAAAAAATGAACCATAAGATCATTTGGTTGTGTAGTTCTGATGAAGCTTGGGATATCTTTTAACCTTATTTCTTTAATGCCTGGTATCCAATCTATGGTAGTTTCCAAATATCCATTTGTCATATAACTGGAATCTTTGAGTGGTGTTAAGCCTCTTTCAACGAGTTGACTATATTGTGTATATGCCATGAAGCCACAAGCACTAGTTGTCCAAAAAAGCACTTCTGGAATATTAAGTTCTTGAGCAGCATCCAAAGTAAAACTCATACAACCATCAGAAACTATGCAAGTAACAGGAGGGGTCTCTATAGCATTATTGAGTTTTGCAAGAAGTTTTTTGAAGTGTGGCAGGCATGTTTTTCGAGTGGAGTCACACAAAGAAGGGATATCCTGTGTGACATCCACATCGGTCTCAGGTAGACCATCAGGAATAGTTTCAAACCGAAAAGAAGATAAACCATTTAGAGAGTTAGGACCTCTTGATTTGAGAAGACGTTTGTGATTGTATTCAGTGTTAACAAAGGTGACATGAAAACCACCTTTAAAATGAAGAAGTTTTGCTAGTTTGAGCATTGGATTTATGTGACCTTGTGCTGGATATGGGATACACACAACATGTGGTTTATTCTTTGTTGTGTTTAGAGAACCCATTTTGTTAGCAACTAATAAAGTTTTTCTTTCAAAGCAGAACTAATTAGCAAAAGAAAGAAGTGATTTGAGTTTGTTCACTCTCCCCGAGTTANNTGNNRNNTAGATGTTTTATAGGTGAAATAGGAATGGCGGTGTAGAATAAAATATTGGTGTCAAGTGGATGGTTGGTGGGGATACTATAGACACGTGGGTTTGTCATGCTCATGGTCCTGCCATAATCCATACCATTATAATATTTTTATTTCAAACTTTTGTAGCCATTGGATTAAGGGCAGCACCAATAGCAAAATGCATTCCACGGGAGACGGGGAGAGGATTATGTGTTCCAAACAGGCAAAGAACTTCATAGATGACATATCGGTCGTTTGGTTCTTCCCTCCCGCCAGTAGACGGTCATTTGGTATTTTAAATTAAATTTTATGTTTTCTATTTTGTTTTTCTAATAAATATAATAATCTATATTATTATTAAAATAAAAGATAATACACCTTCTATCCCAAGAGTCTTTTCAACAAAAATAAGATTATCTTAAAATAAGATTTTTTTCATATTTTCAATCTAAAATTAATTAATTTTTATCAATTTTATATAGAGAATAATTACTATACACTGTCAGTCAGTGTAAGAAGTTTTACACCGTCGATTCATCTCCATCATCCGTTTGTAAGATTTTATAGATTTTTAAAATAAAAGTCAAATTTCTTTTAATATTCGAACGTCTATGATTAACTGGATGGTATAAAATCATTCTACACTTTCAATGTATTTCAATTAAACTCTTTTATATACTATTATCTACATTAGTTTTAAATTATTACATCATATTATATATTTATGACATCAATAATAGTTAAAAACACATTTATAAAATTATTATATTTAATTATTCAATCGTAATTTTTTTAATTTATATACTAAAAACTTAAAAATAAAAGACAGGCAATAATAAATAATTTCAAATTTCAAGTAAATCAAGGATC
TARGET=2161,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03823 Ps_contig_mira-and-tgicl-ass_franssen_31159
SEQUENCE=CTTTGATGCATCAYTTACAAATGACCCATCTCTGTTTTTATGGGTTTCAATGTATTAACTTTTCAAAGTCTAACATAGAGATGACCCATCATTTAAAAATTGTTTAAAATCTGTTAATGTTACACATCAAAGTCTAACATAATATACAAATAAAAGTGATACAACACAACTGTACTAGATTAAACATGAGTCTGCAACTTCCATTCACTTCATTATTATACAATATACAAACAAGAACTATAGAGGCTTTGCACCGCCGCCATTTTCCGCTGCATTATGAACCGGAGTAGACGAACCACTCCTTCTCGACCCGCTATGATGACCTGTTATGGTTCTTCCCATAAACTTTCCTGCCTTGGTAAGACCGCTAGTGAACGTTCCAAGACCACTCGTGACAAGTCCAGCTCCCGCGCCAAGGCCACTTCCAACAAGTCCAGCACCAGATCCCACCACCGACACTGCTCCATCCAGTGCGTCCATTGTGCTACCGATAACCCCCGCCGCTTTCATTTTCTTCCTTTCTTCTAATATTGCTTTTTCCGCTTCTAGTGCAGCCAATTGCTCCTCCTTGTTAAACTGGTAATACAACACCTTTACGGTCAAGGTTCCTCGATCTTTCTTATCTTTAACCTTGAGTGTATCGAGCGATGACAGCAGTCCCAATTGAAACTCCTTTTCGGTTTGTACTTCCAGGTCATTAAGAGGTAATTTCACAATTCCCAATCGCTTATCTTGTCCAATATCTTCGTCAAAAACCTCGAAAATCAGTGACTGAGTCTCCTTGTCTTCTGCAATCAGTTCGAATGTTTGATCCCAAACAGGATTCAAGTTGTTATTAATAACCTTGGTTTTAACTTTGAATAAGGGTCGAATATACAAAACTACGTAAGGATCTGATTTTCCAATCATTTCCTTATTCTTTAAATCGGTCGCTTTCACTACAGTCACTTCAAGTCTTCCTTGAGGTTTAAGCTCCAGTTCACTGATATCAACAGGGATACCGCCGAGTGGAACAACAATCCTATGTGGCCATTGGAGCGTATCATTAACAATTGCGTTCACAGTATCCTCAATCATATCTGAAACTCCGGGAAGAGCAGTTAAGCTTCCACCAACAGCTTTCAGAGTATAATCAATTTTTGGCTTCGGCTCGGCCAATAGAGCAACAACAACAGCAGAAATGCAAGGGATCTCTTCAGCAAGTTGGAATATAACACGGATAATGGTGAAAACTTTTAGATCCTTCAACTGAATAGGGATCGATGCAACATGGGCTTCAACGGCCAAAATAATACTCGGATCACCACCCCAACGGAAATCTATGTCCATTATGATTTGACCTTTGGTAAGACTCTGCACGCGAATACCTTCAACTTTTGGAGCAACATTTCCCAGTGACAGTTTGCTGAACTTCAACGAAGAAATTCCGGGAGGTCGGTACTCTTCCAATAGAGGTTCAACCGACTCTCTGATAACCAACGTTGCGGCTTCTGCCACAAATGGCCAAAGTTTGCTCAGGAGCTTGTTTAGCCATTTCACCTGCTCATATTCAGGGAAAGATATCCATTCGGGAAGATTTTCACCGCAGATTTTCTTCAAATCATCTCTGTTGAGGGATCCAAGGAGCTTGATATCAACCGCCTTTGCAATTCTCTTGGCACTTCTGTATCTCATCATACGAGCCCATCCAGCCATCAATGCTATGCCGAATAACATCCCCATAAAAATCCCAGAAATCAAACCCATTTTTCTAGATTCCAAAAACCACCTTTTTCCCTTAGAAGATGAAGAGCAATTGGAATGAGATTCTTGAAAAGAGACCAAAAAGAGCTCTGAGTTGTTGTGGGACTATAAAAATGTAAACTTTATTGAATTTATAGTCTTCACGAGCTTGAATTGAATTGAATTGAATTGAATGAAAAGAAAGCATTAATAGCTTTGTAGGGACTTCACAGCTTCAATTCAACACTGTTTGCATTCTCAGATTGTAATCTTCCGCGGATTTAGAAAAAGCAATAATTNTCTCCC
TARGET=1892,25
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03824 Ps_contig_mira-and-tgicl-ass_franssen_31164
SEQUENCE=AACACGCCAAGGTGTTTGTGAATGGAATATGCTTCTTGTACTAGCTGTTTCTATATATATATAATGACCAATTANNCRGGGNGACCCCACAAAACATTCAATCTCTTCTTGTCTATAGAAAACTCAATAATGGGGAACCTATCTTCTTCTTCTAACCAAAAAAAGTCTCTTCCCATTGACACCACATTCAAGCTTCCATCTAACATACCAACTTGGCCACAAGGTGGTGGATTTGGTAGTGGAATCATTGATCTAGGAGGTCTAAAAGTGTCACAAATCTCAACATTCAACAAAATTTGGACAACCTTAGAAGGTGGACAAGAAGATTTAGGAGCAACTTTCTTTGAGCCAACACAAATACCTCAAGGGTTCTTCTCATTAGGACACTATAGTCAACCTAACAACAAACCTCTCTTTGGTTGGGTTTTAGTTGCAAAAGATGAGTCCAATGGAGCATTGAAAAGTCCAATTGATTACACACTTGTTTGGAGTAGCAAGTCACAAAAAATCAAACAAGACAAAGATGGTTACATTTGGTTACCAATAGCACCTAATGGCTATAGCCCTTTAGGCCATATTGTCACAACCACACCTGAAAAGCCTTCACTCGATAGAATCCAATGTGTTAGGTCAGATCTCACCGACCAATGTGAAATTAACTCATGGATTTGGGGAAAAGATAAGAAAATTGATGAAAAAGGTTTCAATGTTCATTGTGTTAGACCAAGCAATAGAGGCACACAAGCTCCTAGTGTTCTTGTGGGAACATTCTTAGCACATGTTGGTGAAGTTACAAATAGCCCTTTACCAATTTCTTGTTTGAAAAATTCAAACTTTATGAGTTTTTCATCAATGCCTAATTTACCCCAAGTTAAGGCACTAGCACAAGGCTATTCACCTATCATGTACTTGCATCCAAATGAAAAGTTTCAACCTTGTTCAATTAAATGGTATTTCACAAATGGAGCATTGCTTTATAAGAAAGAAGAAGAAGCAAATCCAATTGAAATAGACCCATTAGGCTCAAATCTTCCTCAAGGTGGAAGCAATGATGGTTCTTATTGGATTGATCTACCAAAAGATAAAGCTAATAGAGAAAGAGTCAAAAAAGGTGATTACAAAAATTTTCAAGCTTATATTCATGTAAAACCAATGTTTGGTGGTACATTTACTGATTTAGCCTTTTGGGTTTTCTACCCTTTTAATGGGCCAGGGACATTAAAAGTTGGGCTTATAGATAACATTTCACTAGGTAAAATAGGTGAACATATTGGTGATTGGGAACATGTTACTTTAAGGATAAGTAACTTTAATGGAGAGTTAAGAAGTGTTTATTTTTCTCAACATAGTAATGGCCAATGGGTTGATGCTTCAGAAGTTGAGTTTCAAAGTGGGAATAAATCAGTGGCTTACTCTTCATTGAATGGTCATGCTATTTATTCAAAAGCTGGACTTGTTCTTCAAGGTGTTAGTGAGATTGGGATAAAGAATGAGACTAAGAAGAGTGAAATGGTTGTTGATTTTGGAGATGGTTTTGAGATTGTATCAGGGGACTATTTGGGGGATGAAGTTGTTGAGCCTAGTTGGTTGAATTTTTTTAGGCAATGGGGTCCTAAATTACTTATAGAAATTTAAAAGGTAAAG
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03825 Ps_contig_mira-and-tgicl-ass_franssen_31164
SEQUENCE=AACACGCCAAGGTGTTTGTGAATGGAATATGCTTCTTGTACTAGCTGTTTCTATATATATATAATGACCAATTANNCRGGGNGACCCCACAAAACATTCAATCTCTTCTTGTCTATAGAAAACTCAATAATGGGGAACCTATCTTCTTCTTCTAACCAAAAAAAGTCTCTTCCCATTGACACCACATTCAAGCTTCCATCTAACATACCAACTTGGCCACAAGGTGGTGGATTTGGTAGTGGAATCATTGATCTAGGAGGTCTAAAAGTGTCACAAATCTCAACATTCAACAAAATTTGGACAACCTTAGAAGGTGGACAAGAAGATTTAGGAGCAACTTTCTTTGAGCCAACACAAATACCTCAAGGGTTCTTCTCATTAGGACACTATAGTCAACCTAACAACAAACCTCTCTTTGGTTGGGTTTTAGTTGCAAAAGATGAGTCCAATGGAGCATTGAAAAGTCCAATTGATTACACACTTGTTTGGAGTAGCAAGTCACAAAAAATCAAACAAGACAAAGATGGTTACATTTGGTTACCAATAGCACCTAATGGCTATAGCCCTTTAGGCCATATTGTCACAACCACACCTGAAAAGCCTTCACTCGATAGAATCCAATGTGTTAGGTCAGATCTCACCGACCAATGTGAAATTAACTCATGGATTTGGGGAAAAGATAAGAAAATTGATGAAAAAGGTTTCAATGTTCATTGTGTTAGACCAAGCAATAGAGGCACACAAGCTCCTAGTGTTCTTGTGGGAACATTCTTAGCACATGTTGGTGAAGTTACAAATAGCCCTTTACCAATTTCTTGTTTGAAAAATTCAAACTTTATGAGTTTTTCATCAATGCCTAATTTACCCCAAGTTAAGGCACTAGCACAAGGCTATTCACCTATCATGTACTTGCATCCAAATGAAAAGTTTCAACCTTGTTCAATTAAATGGTATTTCACAAATGGAGCATTGCTTTATAAGAAAGAAGAAGAAGCAAATCCAATTGAAATAGACCCATTAGGCTCAAATCTTCCTCAAGGTGGAAGCAATGATGGTTCTTATTGGATTGATCTACCAAAAGATAAAGCTAATAGAGAAAGAGTCAAAAAAGGTGATTACAAAAATTTTCAAGCTTATATTCATGTAAAACCAATGTTTGGTGGTACATTTACTGATTTAGCCTTTTGGGTTTTCTACCCTTTTAATGGGCCAGGGACATTAAAAGTTGGGCTTATAGATAACATTTCACTAGGTAAAATAGGTGAACATATTGGTGATTGGGAACATGTTACTTTAAGGATAAGTAACTTTAATGGAGAGTTAAGAAGTGTTTATTTTTCTCAACATAGTAATGGCCAATGGGTTGATGCTTCAGAAGTTGAGTTTCAAAGTGGGAATAAATCAGTGGCTTACTCTTCATTGAATGGTCATGCTATTTATTCAAAAGCTGGACTTGTTCTTCAAGGTGTTAGTGAGATTGGGATAAAGAATGAGACTAAGAAGAGTGAAATGGTTGTTGATTTTGGAGATGGTTTTGAGATTGTATCAGGGGACTATTTGGGGGATGAAGTTGTTGAGCCTAGTTGGTTGAATTTTTTTAGGCAATGGGGTCCTAAATTACTTATAGAAATTTAAAAGGTAAAG
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03826 Ps_contig_mira-and-tgicl-ass_franssen_31164
SEQUENCE=AACACGCCAAGGTGTTTGTGAATGGAATATGCTTCTTGTACTAGCTGTTTCTATATATATATAATGACCAATTANNCRGGGNGACCCCACAAAACATTCAATCTCTTCTTGTCTATAGAAAACTCAATAATGGGGAACCTATCTTCTTCTTCTAACCAAAAAAAGTCTCTTCCCATTGACACCACATTCAAGCTTCCATCTAACATACCAACTTGGCCACAAGGTGGTGGATTTGGTAGTGGAATCATTGATCTAGGAGGTCTAAAAGTGTCACAAATCTCAACATTCAACAAAATTTGGACAACCTTAGAAGGTGGACAAGAAGATTTAGGAGCAACTTTCTTTGAGCCAACACAAATACCTCAAGGGTTCTTCTCATTAGGACACTATAGTCAACCTAACAACAAACCTCTCTTTGGTTGGGTTTTAGTTGCAAAAGATGAGTCCAATGGAGCATTGAAAAGTCCAATTGATTACACACTTGTTTGGAGTAGCAAGTCACAAAAAATCAAACAAGACAAAGATGGTTACATTTGGTTACCAATAGCACCTAATGGCTATAGCCCTTTAGGCCATATTGTCACAACCACACCTGAAAAGCCTTCACTCGATAGAATCCAATGTGTTAGGTCAGATCTCACCGACCAATGTGAAATTAACTCATGGATTTGGGGAAAAGATAAGAAAATTGATGAAAAAGGTTTCAATGTTCATTGTGTTAGACCAAGCAATAGAGGCACACAAGCTCCTAGTGTTCTTGTGGGAACATTCTTAGCACATGTTGGTGAAGTTACAAATAGCCCTTTACCAATTTCTTGTTTGAAAAATTCAAACTTTATGAGTTTTTCATCAATGCCTAATTTACCCCAAGTTAAGGCACTAGCACAAGGCTATTCACCTATCATGTACTTGCATCCAAATGAAAAGTTTCAACCTTGTTCAATTAAATGGTATTTCACAAATGGAGCATTGCTTTATAAGAAAGAAGAAGAAGCAAATCCAATTGAAATAGACCCATTAGGCTCAAATCTTCCTCAAGGTGGAAGCAATGATGGTTCTTATTGGATTGATCTACCAAAAGATAAAGCTAATAGAGAAAGAGTCAAAAAAGGTGATTACAAAAATTTTCAAGCTTATATTCATGTAAAACCAATGTTTGGTGGTACATTTACTGATTTAGCCTTTTGGGTTTTCTACCCTTTTAATGGGCCAGGGACATTAAAAGTTGGGCTTATAGATAACATTTCACTAGGTAAAATAGGTGAACATATTGGTGATTGGGAACATGTTACTTTAAGGATAAGTAACTTTAATGGAGAGTTAAGAAGTGTTTATTTTTCTCAACATAGTAATGGCCAATGGGTTGATGCTTCAGAAGTTGAGTTTCAAAGTGGGAATAAATCAGTGGCTTACTCTTCATTGAATGGTCATGCTATTTATTCAAAAGCTGGACTTGTTCTTCAAGGTGTTAGTGAGATTGGGATAAAGAATGAGACTAAGAAGAGTGAAATGGTTGTTGATTTTGGAGATGGTTTTGAGATTGTATCAGGGGACTATTTGGGGGATGAAGTTGTTGAGCCTAGTTGGTTGAATTTTTTTAGGCAATGGGGTCCTAAATTACTTATAGAAATTTAAAAGGTAAAG
TARGET=983,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03827 Ps_contig_mira-and-tgicl-ass_franssen_31170
SEQUENCE=TAACGCAGAGTATGTTTCTTTTTCTGTGTTTTATTTTACTTTTTAGGTTTTCTTTTAATGTGGCAAACTCCGTTACAGTGCTGTGGTTATTGGGATTTAAATTACCAGGAGGCTTTGAAGAAAGATAGCGGTGGATGGAAAAAAGTCGTTGCGGCGTTCGGGGAGGAAATTCTTCTAGACAATGGAGAAGTTAACAGACCCAGACTTGGCCAAATCGTTTTTGCGGATCCTGATAAGCGTCAATTTCTCAACCGGTATTCTAAATTTATGTTGCGTGAATTATATTACAATTAATTTAATTTAATTTTTTTACTTTGAAGTTCTGGTTGTGATTGTTGCTTTAGATTGTTGGCTCCTTATATATCGTCGGGGATCTTTTGGGAAGTTGGGAAGCTATGGTTGAAAGGTTACAAGGTCATTGTTCTTGATGTCCCTTTGTTATTTGAGGCCAAGATAGACAAGTTCACGACGCCCATTATTGTCGTGTGGGTTGATCCTGAAACACAGATTCAGAGACTCTTAGCAAGAGACAAATCTAGTGAGGAGGATGGTCGGAATAGGGTTAATGCTCAGATGCCACTGGATGATAAAAAGAGTAAAGCTGATATAGTAATAGACAACACCGGTTCACTAGATGACTTGAATGAACAATTTCAGAAGGTTTTGTTCCGGGTCACTGGACCCTTGACATGGTGTGAATTTTGGCGTTCTAGGCAGGGGGTCTCGATCATTCTTGCTTCACTTTCCTCAGGTGTTGTTCTATGTGCGAAGGCATTCAACAACAACAGGTCATAGACACCTTCCTATATTGTGTTTTGTGAAGTGCTTCACTGCATCTCTTTCTAAGTTCCCAACAAATAGATAGTACCATACTACAACCTGAGTTTCAAGATGATATTAGAATTTACTTGTTTATTATAGGATTTGTTACTGTTAGGGAACTCCAAAAGACAAGATATGGAATATTGATAATGGTAATCACAGGTAATTTGTTTGCCCATAGACCTGTCAAACCATTGGCTTGCAATGAGTGGAAGATAACCTGGTGTCCATTATATCATATAGTGCAGTATTGATATGGATGTTTTTGTTCTAATGTTGAATGTTACTGGCTTYAATATTTTGCACTTAGCAAGTAGGTGCATGCATGATTAATTCATTATCCTTCATACCCAATTCAACAGCATAAATAAATACATGTCTCAGATAAGGATCAATTGGCTTACTTGTT
TARGET=291,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03828 Ps_contig_mira-and-tgicl-ass_franssen_31173
SEQUENCE=GGGGACAACAACAAGAATCACCTTCTGTACCCAAAAAATTCAATTTGACTTCTTTTTCTCTAACCCTTCTTCTTCTTCTTCTTCTTCACCGATTCTTCTGAACCTGAAAATACTACAACATTGCCACATGAATAATTAGGGTTTACACACAACAAAGTTGAAACGATTTCCGAAATGAGCGGTTCCAGATTCATAAAGTGTGTCACAGTAGGCGATGGAGCCGTTGGCAAGACTTGTTTGCTTATCTCCTATACCAGCAACACTTTCCCAACGGACTATGTGCCCACTGTCTTTGACAATTTCAGTGCAAATGTAGTTGTGGATGGGAGCACTATAAATTTGGGGTTGTGGGATACAGCTGGCCAAGAAGATTACAATAGATTAAGACCCTTAAGCTATCGTGGAGCCGATGTTTTCTTGCTTGCCTTTTCTCTCATAAGTAGAGCTAGTTATGAGAATATTGCCAAGAAATGGATTCCTGAGTTAAGGCATTATGCTCCCGGTGTTCCAATTATTCTCGTTGGAACAAAACTTGATCTTCGAGATGATAACCAGTTCTTTCAAGATCATCCTGGTGCTGCACCAATCACCACAGCACAGGGTGAGGAACTCAGAAAACTTATTGGTGCTCCAGTTTACATAGAATGTAGTTCCAAAACACAAAAGAATGTGAAGGCTGTTTTCGATGCGGCCATCAAAGTAGTTCTACAGCCGCCAAAACAAAAGAAACCTAAGAGAAAGGGGCATAAAGCCTGTTCTATTTTGTGATCTTCAATTCTTCATTTTGTAGTTCATGGAGGATGACATGACAGTAGCCATTTCCTGGTAGTGTTTGCCATTTCTGATTCCATATCTTACACTATTTTCAACCTAGATGGAGGAATTCACCATGTTCAATTGACCACGAAAAGGTTTTGCTTATAAAGCATCAAGCTATTTGTCTCTGGCTGAAGGATATACTATTTAAAGTCATAATTTCAACATGTTTGGTGTGTACAAACTCTTGTGTTTCTCTGGGATATTTACCTGCTGTATTGATTATTAAATATCCATCTCCACTTGTGGACATGGGTTTTACTCACATTGTAATCTAATCTAGGAAGTGTTTGCATATGTGGCTTTGGATGTGCACTAAGTATATGTAATTAATTATGTCAGCTGTTTAACAGCCTGAATATAGTTTGGTTTGTTCATTGATTCTATTACTTNNATTTTAGTTT
TARGET=66,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3633
PRIMER_LEFT_PENALTY=0.267873
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=GGGACAACAACAAGAATCACCT
PRIMER_RIGHT_SEQUENCE=CTTTATGAATCTGGAACCGCTC
PRIMER_LEFT=1,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=60.268
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=0.3756
PRIMER_LEFT_1_PENALTY=0.267873
PRIMER_RIGHT_1_PENALTY=0.107763
PRIMER_LEFT_1_SEQUENCE=GGGACAACAACAAGAATCACCT
PRIMER_RIGHT_1_SEQUENCE=GGGAAAGTGTTGCTGGTATAGG
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=268,22
PRIMER_LEFT_1_TM=60.268
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.3774
PRIMER_LEFT_2_PENALTY=0.267873
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=GGGACAACAACAAGAATCACCT
PRIMER_RIGHT_2_SEQUENCE=TTTCAGGTTCAGAAGAATCGGT
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=108,22
PRIMER_LEFT_2_TM=60.268
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.4731
PRIMER_LEFT_3_PENALTY=0.267873
PRIMER_RIGHT_3_PENALTY=0.205226
PRIMER_LEFT_3_SEQUENCE=GGGACAACAACAAGAATCACCT
PRIMER_RIGHT_3_SEQUENCE=ACATAGTCCGTTGGGAAAGTGT
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=280,22
PRIMER_LEFT_3_TM=60.268
PRIMER_RIGHT_3_TM=59.795
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.5426
PRIMER_LEFT_4_PENALTY=0.267873
PRIMER_RIGHT_4_PENALTY=0.274768
PRIMER_LEFT_4_SEQUENCE=GGGACAACAACAAGAATCACCT
PRIMER_RIGHT_4_SEQUENCE=ATAGTCCGTTGGGAAAGTGTTG
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=278,22
PRIMER_LEFT_4_TM=60.268
PRIMER_RIGHT_4_TM=60.275
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_03829 Ps_contig_mira-and-tgicl-ass_franssen_31175
SEQUENCE=ATTTTTTTTGCACCAAACAAATAAARTNGAGGNGAGTAACGAGTTGAGTTGACGAACGAGTTGCGAAGGAAGAAGAAGAAGCAGAAGAAGATGAGCGAAACAAGGCCAGTTCCTCGGAGAGAGAGTCCATGGGGGATAACAGGAGAGAATCACCCTGAACCTAAAGCTCATCGATGCAATGATCGCGTTGAAGATGTTATCCAGGCTTGCTTTGAGGGAAACCCATTCAAGACTGTTCCAGGACCATTCAAGCTCTTCTGGCAGTGCATGCGTTCTAAACCTGGCGAGGAACCAACTGAGCCATTTACCTATTTGGATTTGGATCCTCCAAAGAGAGAGACCGTAAAGCTTGAGTAGTGCCTCTTTTGCAAACGCCTTTAGAACAGCGTTGGCATGGTCATTTTGTGGTTTCAACCTTTCTTTCCATGTTGTGGATAGGTTATGCAAAGATTATATATCTAGTTAATTGTCTTTGTTGCTTACTTCAATTTGCAAATTAGACATCCTTTGTATTTGTTCCACACTTGATGTACCAGCCCAATAAAAAGTGCCAGAGCTTTGTTTTTGAATTACATGATAGTTTGTTATAATTACTTGTCTGATGATATAAATTCCACCAGTTTATGTTGGCTCAAAGT
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03830 Ps_contig_mira-and-tgicl-ass_franssen_31179
SEQUENCE=AAACAGTTTTTCCAGTTTATAGCGTAAGGAGTGAGCGAAAAAGTGAGAAATCCTTAGCTTCTTCTTCTTCCTTCAGAAAAACTCAATTCACATTATCATCATCACTTCTTTGCAATCGATTCCATTTCCGTTCTCAACATGGAAAGCGCATCAGAGCTCGTTGAATTTCCATTGTTGTTAACACCAATTCATTCTCATTACAGGGCTTGCACTATTCCTTATAGATTCCCTTCTGATAATCCTCGAAAACCTACTCCCAACGAAATCTCATGGATTAATCTCTTCCTTAACTCAATACCCTCTTTCAAGAAGCGTGCAGAGAGCGATACTTCTGTTCCCGATGCTGCAAATAAAGCTGAAAAATTTGCTCAAAGGTATGCTGATATACTTGAAGATTTCAAGAAAGATCCCGAAAGTCATGGTGGACCACCCGATTGCATTCTTCTATGCAGACTTCGCGAGCTAATACTCAGAGAATTGGGATTCAGTGATATCTTCAAAAAAGTCAAGGATGAAGAAAATGCAAACGCCATCTCATTGTTTGAGAATGTTGTTCTTGTTAATGACGCCATTGAAGATGAAGGCAAGCGAATTGAGAATCTAGTTAGGGGAATTTTTGCAGGAAACATATTTGATCTTGGCTCAGCGCAGCTTGCAGAGGTTTTCTCTAAGGATGGAATGTCCTTTTTGGCAAGTTGTCAAAATCTTGTACCTCGACCTTGGGTTATTGATGATCTTGATGATTTCAAAGTGAAATGGAGCAAAAAGTCATGGAAGAAGGTTATTATATTTGTCGATAATTCTGGTGCTGATATTATTTTGGGCATTTTACCATTTGCAAGGGAGCTTCTTCGGCGTGGGAGTCAGGTTGTATTGGCTGCAAATGACTTACCTTCCATCAACGATGTGACATATTCTGAGCTAGTTGAAATCATTTCAAAGTTAAAGGACGAAGAAGGAAAACTTGTCGGTGTCAGTTCATCAAACCTTTTAATTGCCAACTCTGGCAATGATTTACCGGTTATTGATCTTACAAGAGTGTCACAAGAGCTTGCTTACCTTGCTAGTGATGCAGATCTTGTCATCTTAGAAGGGATGGGCCGAGGAATCGAAACAAATCTGTATGCTCAATTTAAATGTGATTCCCTTAAGATTGGAATGGTAAAACATCCTGAGGTTGCACAATTTCTCGGAGGACGCCTATACGACTGTGTATTCAAATACAATGAAGTTTCGAGTTGATAAATCTCATTCTTTTGTCTGCTAATATGCTCTACATACTCTCGGTATTGGCCTGCCTGAAACTAGATGCTGTCTAGAATGTCATTTTAGTATTTGAAAATGGCGACAAAACTTGACATTTTTACTTGTAATAACAAAGTTATTCATTCTACCTACATACATGGCTACTTCTTTGTAAGACTGTATGTTACTGATACATACAACATGTGCGATAAGAGTCAACCGGTTAAACTTGCAATCATAAGTACCATTAATTTTNACG
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0544
PRIMER_LEFT_PENALTY=0.052069
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=CGTAAGGAGTGAGCGAAAAAGT
PRIMER_RIGHT_SEQUENCE=TGAGATTTCGTTGGGAGTAGGT
PRIMER_LEFT=22,22
PRIMER_RIGHT=269,22
PRIMER_LEFT_TM=59.948
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.0563
PRIMER_LEFT_1_PENALTY=0.054021
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=AAGGAGTGAGCGAAAAAGTGAG
PRIMER_RIGHT_1_SEQUENCE=TGAGATTTCGTTGGGAGTAGGT
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=269,22
PRIMER_LEFT_1_TM=60.054
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.0860
PRIMER_LEFT_2_PENALTY=0.052069
PRIMER_RIGHT_2_PENALTY=0.033905
PRIMER_LEFT_2_SEQUENCE=CGTAAGGAGTGAGCGAAAAAGT
PRIMER_RIGHT_2_SEQUENCE=TTCGAGGATTATCAGAAGGGAA
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=59.948
PRIMER_RIGHT_2_TM=60.034
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=0.0860
PRIMER_LEFT_3_PENALTY=0.052069
PRIMER_RIGHT_3_PENALTY=0.033905
PRIMER_LEFT_3_SEQUENCE=CGTAAGGAGTGAGCGAAAAAGT
PRIMER_RIGHT_3_SEQUENCE=TTTCGAGGATTATCAGAAGGGA
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=247,22
PRIMER_LEFT_3_TM=59.948
PRIMER_RIGHT_3_TM=60.034
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.0879
PRIMER_LEFT_4_PENALTY=0.054021
PRIMER_RIGHT_4_PENALTY=0.033905
PRIMER_LEFT_4_SEQUENCE=AAGGAGTGAGCGAAAAAGTGAG
PRIMER_RIGHT_4_SEQUENCE=TTCGAGGATTATCAGAAGGGAA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=60.054
PRIMER_RIGHT_4_TM=60.034
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_03831 Ps_contig_mira-and-tgicl-ass_franssen_31185
SEQUENCE=AACGACGRGGATNCTRTGATACCACTCCAGCAATCGCTAATCAAAACTTGAATCCCTAACATTCCAATCCCAATCGATAGGCATTGGGTTTAATGGCTTCAAAACGTATCACTAAGGAGTTGAAGGATCTGCAGAAAGACCCTCCCGTGTCCTGCAGTGCTGGCCCTGTTGGGGAAGACTTGTTTCATTGGCAAGCTACAATCATGGGACCAGCAGACAGTCCTTATGCTGGTGGTGTATTCCTAGTGACCATTCACTTCCCACCAGATTATCCATTTAAGCCGCCAAAGGTTTCCTTCCGAACAAAAGTGTTTCACCCTAACATAAACAGCAATGGAAGTATCTGTCTTGACATTCTGAAAGAACAGTGGAGCCCGGCTCTTACTGTTTCCAAGGTGCTGCTGTCTATTTGCTCCCTGCTGACAGATCCTAACCCAGATGATCCTTTGGTGCCTGATATTGCCCACATGTACAAGACTGACAAAGCCAAGTACGAAAGTACTGCCCGGTCTTGGACCCAGAAATATGCAATGAACTAGCACAAATATAGTGGACTCTTTCTGTGGGGGAAAATGTTGTTTGGGGATGCCTGCTGGGTATATATACATTACATATATAAGTGCATCTGCTATGTTTCTATAATAATAATAATCCAAACTTTCAAGTAAANTTTAAGGGCTTNARGNTAANATTTAANATTNAAAAAAAAATTT
TARGET=639,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03832 Ps_contig_mira-and-tgicl-ass_franssen_31186
SEQUENCE=TTTAAATAAGGAAAGCAATTCATCTGATGATTATTATTAGTTCGTTTACACTCGTGCTCGTTGGCTGTCGTGCCAACAATCATTTTCCAAAACAAAACACTCCATCAATCGGCATATCACTAAAGTACATAATTTAGTATGTGTTTGTGACTGCATCACATTGACTCATATTACTTCAACAAACCACATTGATGTGAAGCTAAGCCTTAGAGCTTCTAAAAAGTAAACCAAACACTATTACATTACATTACATTCATTACGAAAGCAATACCACAAATTATGAAACATTTGATGCATTGACTGGGATTTTTTCAGCAGATGGTGTTTCCTTGAAGCTCAGCATCTTGTTTATTGCAGCAATATAGGCCTTGACACTTGAAACAACAACATCCATTCCTGCTCCAGTCCCACTAAAAGTTCGCTGAACACTTTCTCCAGTTAACGTGTGAGTAGATGCATGATTGCTTTCCCCCCGAATTAAAACTCGGGTAGTCGCAATTGCATCAATGCCTTCAGTGACTGCGACCATGGAGTATTCAAGAAGAGTTGCTGGTTCCTTCACAATCAGATCAACAGCTTTGTACGCTGAATCCACAGGCCCAGTACCAACAGAACAAGCAACGTGCGTGCTACCGTCATCACCAACAAGTTTAACTGTTGCTGTTGAGAGACCAAGAGTTCCACAAGTCACCTGTATATCAGCAAGCTTCCATATAGGCTCTGCCTGAAAAACTTCATCCGATACCAATGCTCTGAGATCAGCATCAGTAATCCTCTTCTTTTGCTCAGCCACAGCTTTGAAACGCCAAAACAAACTCTCAACTTGGTCGTCTTTAAGTTCGTATCCAAGCTCTTCAAGTCGCTTCCTCATAGCATGGCGTCCGCTAAGTTTCCCCAATACAATACCAGCTTCATTGGTTCTTTCAAGTCCAATATCCTCCGGTGATATGATTTCGTATGTACCTTTGTGTTTAAGCATTCCATCCTGATGTATGCCACTTTCATGAGCAAAAGCATTAGCTCCCACAAGAGCCTTGTGTGGTTGTAACTGCAAACCAGAGTACTCTTCAACCATTTTGCTTGTCATATAAATGTGCCTTGTATTGATTCCAGTGTGGAGATTGTTGAAGATATGTCCTCCGCATTTCAGGGCCATCACAACCTCTTCCAAGGAGGCATTTCCAGCCCTTTCACCAATCCCATTAATGGTGACTTCCAGCTGCCTAGCACCGGCTCGAGCACCCTCAATGGTGTTAGCAGTAGAAAGTCCAAGATCATTCTGACAGTGAGTGGAAATAATAACATTCTCCACTCCGGGTGTATTAGCTTTTATATCAGCAATCAGTTTCCCGAATTCACTCGGCATAGTTATTCCAACAGTGTCAGGTATGTTGAGTGTAGTAGCCCCTGCCTTAATAACTTCTCCAAGAATTTGATACAGAAACTCTCTATCCGATCTTCCAGCATCTTCGGGACTGAACTCAACGTCTTGGCAGCCTAAACTACGGGCGAATGCGACCATACTGCGTGCTTTTTCAATGACCTGTTCTCTGGTCATTCTGAGTTTATACTCCATATGAATGGAGCTAGTAGCAATGAAAGTGTGAACCCTTGGTCTCTTAGCATGTTTAACTGCATCCCAAGCCCTTTGAATATCCTTCTCATTACATCTGGAGAGACCACAAATGACAGGGACATAACCGTTTTCGTCGACAGCGTTTCCGACTTCAGTGGCAATCATTTTAACGGCTTCGAAGTCGTCTTTAGAGGCAGCGGGAAATCCAGCCTCAATGATATCTACACCAAGCTTCGCAAGCTGGCGAGCAATGTCGAGTTTTTCCTTGGAAGTCATGGAGGCTCCGGGGGACTGTTCGCCGTCGCGGAGAGTGGTGTCGAAGATGCGAACATAGGAAGAGTCAGGGATGTGGTTGGGGATGTAAGGAGGACGTTGGCGAAGGGGTTCTGGTTGGGATTGGGGTTGAGATTGAGATTGAGACTGAGAGGAGCAGGAAATGGAGAATTTGGGTTTAGAGGAATGAGGGAAATGGAGGCGGAGGAGAGGTTGGTTTTGTGAAGAGGGAGAGCGTGTGGTGGTGGTGGGAGAGAAGAAGGGAGTTCGAATTGCGGTGGCCATGGAAATGGAATAGAAACCCTAGATGAGAAACAGAAGAAGAAGGAGAAGATGAATAGTGGATTGAAATTGAATATTCCAACAAAATAAGTGATGT
TARGET=237,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1211
PRIMER_LEFT_PENALTY=0.095523
PRIMER_RIGHT_PENALTY=0.025566
PRIMER_LEFT_SEQUENCE=ACAAACCACATTGATGTGAAGC
PRIMER_RIGHT_SEQUENCE=AGATGCTGAGCTTCAAGGAAAC
PRIMER_LEFT=178,22
PRIMER_RIGHT=344,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.1672
PRIMER_LEFT_1_PENALTY=0.095523
PRIMER_RIGHT_1_PENALTY=0.071684
PRIMER_LEFT_1_SEQUENCE=ACAAACCACATTGATGTGAAGC
PRIMER_RIGHT_1_SEQUENCE=GGAGAAAGTGTTCAGCGAACTT
PRIMER_LEFT_1=178,22
PRIMER_RIGHT_1=435,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.2092
PRIMER_LEFT_2_PENALTY=0.183585
PRIMER_RIGHT_2_PENALTY=0.025566
PRIMER_LEFT_2_SEQUENCE=CCATCAATCGGCATATCACTAA
PRIMER_RIGHT_2_SEQUENCE=AGATGCTGAGCTTCAAGGAAAC
PRIMER_LEFT_2=101,22
PRIMER_RIGHT_2=344,22
PRIMER_LEFT_2_TM=59.816
PRIMER_RIGHT_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.2529
PRIMER_LEFT_3_PENALTY=0.095523
PRIMER_RIGHT_3_PENALTY=0.157415
PRIMER_LEFT_3_SEQUENCE=ACAAACCACATTGATGTGAAGC
PRIMER_RIGHT_3_SEQUENCE=CGAACTTTTAGTGGGACTGGAG
PRIMER_LEFT_3=178,22
PRIMER_RIGHT_3=420,22
PRIMER_LEFT_3_TM=59.904
PRIMER_RIGHT_3_TM=60.157
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.2601
PRIMER_LEFT_4_PENALTY=0.095523
PRIMER_RIGHT_4_PENALTY=0.164560
PRIMER_LEFT_4_SEQUENCE=ACAAACCACATTGATGTGAAGC
PRIMER_RIGHT_4_SEQUENCE=AACTTTTAGTGGGACTGGAGCA
PRIMER_LEFT_4=178,22
PRIMER_RIGHT_4=418,22
PRIMER_LEFT_4_TM=59.904
PRIMER_RIGHT_4_TM=60.165
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_03833 Ps_contig_mira-and-tgicl-ass_franssen_31186
SEQUENCE=TTTAAATAAGGAAAGCAATTCATCTGATGATTATTATTAGTTCGTTTACACTCGTGCTCGTTGGCTGTCGTGCCAACAATCATTTTCCAAAACAAAACACTCCATCAATCGGCATATCACTAAAGTACATAATTTAGTATGTGTTTGTGACTGCATCACATTGACTCATATTACTTCAACAAACCACATTGATGTGAAGCTAAGCCTTAGAGCTTCTAAAAAGTAAACCAAACACTATTACATTACATTACATTCATTACGAAAGCAATACCACAAATTATGAAACATTTGATGCATTGACTGGGATTTTTTCAGCAGATGGTGTTTCCTTGAAGCTCAGCATCTTGTTTATTGCAGCAATATAGGCCTTGACACTTGAAACAACAACATCCATTCCTGCTCCAGTCCCACTAAAAGTTCGCTGAACACTTTCTCCAGTTAACGTGTGAGTAGATGCATGATTGCTTTCCCCCCGAATTAAAACTCGGGTAGTCGCAATTGCATCAATGCCTTCAGTGACTGCGACCATGGAGTATTCAAGAAGAGTTGCTGGTTCCTTCACAATCAGATCAACAGCTTTGTACGCTGAATCCACAGGCCCAGTACCAACAGAACAAGCAACGTGCGTGCTACCGTCATCACCAACAAGTTTAACTGTTGCTGTTGAGAGACCAAGAGTTCCACAAGTCACCTGTATATCAGCAAGCTTCCATATAGGCTCTGCCTGAAAAACTTCATCCGATACCAATGCTCTGAGATCAGCATCAGTAATCCTCTTCTTTTGCTCAGCCACAGCTTTGAAACGCCAAAACAAACTCTCAACTTGGTCGTCTTTAAGTTCGTATCCAAGCTCTTCAAGTCGCTTCCTCATAGCATGGCGTCCGCTAAGTTTCCCCAATACAATACCAGCTTCATTGGTTCTTTCAAGTCCAATATCCTCCGGTGATATGATTTCGTATGTACCTTTGTGTTTAAGCATTCCATCCTGATGTATGCCACTTTCATGAGCAAAAGCATTAGCTCCCACAAGAGCCTTGTGTGGTTGTAACTGCAAACCAGAGTACTCTTCAACCATTTTGCTTGTCATATAAATGTGCCTTGTATTGATTCCAGTGTGGAGATTGTTGAAGATATGTCCTCCGCATTTCAGGGCCATCACAACCTCTTCCAAGGAGGCATTTCCAGCCCTTTCACCAATCCCATTAATGGTGACTTCCAGCTGCCTAGCACCGGCTCGAGCACCCTCAATGGTGTTAGCAGTAGAAAGTCCAAGATCATTCTGACAGTGAGTGGAAATAATAACATTCTCCACTCCGGGTGTATTAGCTTTTATATCAGCAATCAGTTTCCCGAATTCACTCGGCATAGTTATTCCAACAGTGTCAGGTATGTTGAGTGTAGTAGCCCCTGCCTTAATAACTTCTCCAAGAATTTGATACAGAAACTCTCTATCCGATCTTCCAGCATCTTCGGGACTGAACTCAACGTCTTGGCAGCCTAAACTACGGGCGAATGCGACCATACTGCGTGCTTTTTCAATGACCTGTTCTCTGGTCATTCTGAGTTTATACTCCATATGAATGGAGCTAGTAGCAATGAAAGTGTGAACCCTTGGTCTCTTAGCATGTTTAACTGCATCCCAAGCCCTTTGAATATCCTTCTCATTACATCTGGAGAGACCACAAATGACAGGGACATAACCGTTTTCGTCGACAGCGTTTCCGACTTCAGTGGCAATCATTTTAACGGCTTCGAAGTCGTCTTTAGAGGCAGCGGGAAATCCAGCCTCAATGATATCTACACCAAGCTTCGCAAGCTGGCGAGCAATGTCGAGTTTTTCCTTGGAAGTCATGGAGGCTCCGGGGGACTGTTCGCCGTCGCGGAGAGTGGTGTCGAAGATGCGAACATAGGAAGAGTCAGGGATGTGGTTGGGGATGTAAGGAGGACGTTGGCGAAGGGGTTCTGGTTGGGATTGGGGTTGAGATTGAGATTGAGACTGAGAGGAGCAGGAAATGGAGAATTTGGGTTTAGAGGAATGAGGGAAATGGAGGCGGAGGAGAGGTTGGTTTTGTGAAGAGGGAGAGCGTGTGGTGGTGGTGGGAGAGAAGAAGGGAGTTCGAATTGCGGTGGCCATGGAAATGGAATAGAAACCCTAGATGAGAAACAGAAGAAGAAGGAGAAGATGAATAGTGGATTGAAATTGAATATTCCAACAAAATAAGTGATGT
TARGET=2088,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6573
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.651681
PRIMER_LEFT_SEQUENCE=TAGGAAGAGTCAGGGATGTGGT
PRIMER_RIGHT_SEQUENCE=CAATTCGAACTCCCTTCTTCTC
PRIMER_LEFT=1907,22
PRIMER_RIGHT=2123,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=59.348
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.7129
PRIMER_LEFT_1_PENALTY=0.061184
PRIMER_RIGHT_1_PENALTY=0.651681
PRIMER_LEFT_1_SEQUENCE=TGGAGAATTTGGGTTTAGAGGA
PRIMER_RIGHT_1_SEQUENCE=CAATTCGAACTCCCTTCTTCTC
PRIMER_LEFT_1=2013,22
PRIMER_RIGHT_1=2123,22
PRIMER_LEFT_1_TM=59.939
PRIMER_RIGHT_1_TM=59.348
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=0.7956
PRIMER_LEFT_2_PENALTY=0.143968
PRIMER_RIGHT_2_PENALTY=0.651681
PRIMER_LEFT_2_SEQUENCE=TGTCGAGTTTTTCCTTGGAAGT
PRIMER_RIGHT_2_SEQUENCE=CAATTCGAACTCCCTTCTTCTC
PRIMER_LEFT_2=1827,22
PRIMER_RIGHT_2=2123,22
PRIMER_LEFT_2_TM=60.144
PRIMER_RIGHT_2_TM=59.348
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=0.8386
PRIMER_LEFT_3_PENALTY=0.186949
PRIMER_RIGHT_3_PENALTY=0.651681
PRIMER_LEFT_3_SEQUENCE=ATTGGGGTTGAGATTGAGATTG
PRIMER_RIGHT_3_SEQUENCE=CAATTCGAACTCCCTTCTTCTC
PRIMER_LEFT_3=1971,22
PRIMER_RIGHT_3=2123,22
PRIMER_LEFT_3_TM=60.187
PRIMER_RIGHT_3_TM=59.348
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=153
PRIMER_PAIR_PENALTY_4=1.0203
PRIMER_LEFT_4_PENALTY=0.368624
PRIMER_RIGHT_4_PENALTY=0.651681
PRIMER_LEFT_4_SEQUENCE=CGAGTTTTTCCTTGGAAGTCAT
PRIMER_RIGHT_4_SEQUENCE=CAATTCGAACTCCCTTCTTCTC
PRIMER_LEFT_4=1830,22
PRIMER_RIGHT_4=2123,22
PRIMER_LEFT_4_TM=59.631
PRIMER_RIGHT_4_TM=59.348
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_03834 Ps_contig_mira-and-tgicl-ass_franssen_31189
SEQUENCE=CGGTAACGGGTTAGTACATTTTACTTGGCGTTAGCTAGTTTCTNGATTCTCATTCTCTTCACAATGGCTCTCACTTGTCATTCTCCTCAACTATCCATCTCTCCATTCTCTCGCTCAACCCAATTCAACAGTCGTTGTTACACATTCATCACACACACTTCCCCAACCGTTACTTCCCGCCGAATTATCCCCATTCCCAACAACAACAACAACAACAACAACAACAACAAAACCCTCTTCACTCTTTCTCTCACTAAACCAACTCCATCTTTCTCTGTAACGGCTTCTTCTAACAACACACAACCAATGCTTCCCCCTTACAACGTTTTGATTACCGGCTCAACCAAAGGGATAGGGTTTGCTTTAGCAAAGGAGTTTCTAAAAGCAGGGGACAATGTCTTGGTTTGCTCAAGATCAGATAAAAGGGTAGAGACTGCAGTCAAGTGCTTGAGAGAAGAATTTGGAGAGCAGCATGTGTGGGGCACTACATGTGATGTCAAAAATGGAGAGGATGTGAAGAAATTAGTTTCGTTTGCTAAAGAAAATCTGAAATACATTGACATATGGATCAATAATGCTGGATCAAATGCATATAGCTACAAGCCACTTGCTGAAGCATCAGATGAGGATCTTATTGAGGTGGTTACAACAAACACACTTGGCTTGATGATATGCTGTCGAGAGGCAATAAAGTTGATGGTGGACCAACCTCGCGGAGGACATGTTTTCAACATTGACGGAGCAGGTTCAGATGGAAGACCAACTCCCAGGTTTGCTGCATATGGAGCAACAAAGAGAAGCGTGGTTCATTTAACAAAGTCATTACAGGCCGAATTGCAGATGCAAGATGTGAAAAACGTCATGGTTCACAATCTTTCGCCAGGAATGGTTACAACTGATCTTCTCATGTCTGGTGCCAATACAAAACAGGCAAAGTTTTTCATTAATGTCTTGGCTGAACCAGCCGAAGTGGTTGCAGAATACTTAGTTCCAAATATTAGATCTATCCCTACCAACAGATCAATGAAGCCGACATACATCCGGTTCCTCACGGGTTTGAAAGCCTATTCACAGATATTTTCAAGATTTGCATTTGGGGCAAGGAGGAACCGGTACATTATCGAAGATTGAACAGCATATACATAGATGCCATGTTCGATTCTTGTCGGCCCCTTTATTTTATGATTTTCTCTTTTCTTTTTCTCCTTTCTGTAAATCTGAAATCAACATATATATGCTATTGCTATTATTATTAGAGAAAAAAAGAATATACACTTAGAGGGTAAAATATTTTTATTAATAAGTGATTTTCAATT
TARGET=198,33
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1026
PRIMER_LEFT_PENALTY=0.101896
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=CCCAATTCAACAGTCGTTGTTA
PRIMER_RIGHT_SEQUENCE=AAGACATTGTCCCCTGCTTTTA
PRIMER_LEFT=118,22
PRIMER_RIGHT=400,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.1077
PRIMER_LEFT_1_PENALTY=0.101896
PRIMER_RIGHT_1_PENALTY=0.005761
PRIMER_LEFT_1_SEQUENCE=CCCAATTCAACAGTCGTTGTTA
PRIMER_RIGHT_1_SEQUENCE=GCTAAAGCAAACCCTATCCCTT
PRIMER_LEFT_1=118,22
PRIMER_RIGHT_1=367,22
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.1593
PRIMER_LEFT_2_PENALTY=0.101896
PRIMER_RIGHT_2_PENALTY=0.057411
PRIMER_LEFT_2_SEQUENCE=CCCAATTCAACAGTCGTTGTTA
PRIMER_RIGHT_2_SEQUENCE=GAAGCATTGGTTGTGTGTTGTT
PRIMER_LEFT_2=118,22
PRIMER_RIGHT_2=312,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.1726
PRIMER_LEFT_3_PENALTY=0.115189
PRIMER_RIGHT_3_PENALTY=0.057411
PRIMER_LEFT_3_SEQUENCE=GGCTCTCACTTGTCATTCTCCT
PRIMER_RIGHT_3_SEQUENCE=GAAGCATTGGTTGTGTGTTGTT
PRIMER_LEFT_3=65,22
PRIMER_RIGHT_3=312,22
PRIMER_LEFT_3_TM=59.885
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=248
PRIMER_PAIR_PENALTY_4=0.2170
PRIMER_LEFT_4_PENALTY=0.115189
PRIMER_RIGHT_4_PENALTY=0.101809
PRIMER_LEFT_4_SEQUENCE=GGCTCTCACTTGTCATTCTCCT
PRIMER_RIGHT_4_SEQUENCE=TAATCAAAACGTTGTAAGGGGG
PRIMER_LEFT_4=65,22
PRIMER_RIGHT_4=333,22
PRIMER_LEFT_4_TM=59.885
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=12.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_03835 Ps_contig_mira-and-tgicl-ass_franssen_31190
SEQUENCE=GTCGGTGATAGATAGTAGAGAGATGATGATGGCTCATAGNATCACTCAAGAACAAACCATTCACTTCAACACAAAAAATGGCTTCAATCACCAAAGTGATTCTTCTTTATTCTCCTCTTACCTCCAAAACTTCATTTCTTCCTAAATCACCTTCACTTTCTCACACCATTCTCTCTCCCAAAACAAGTCTCAAAGTTCATGCTCAATTAGGTGGTCGAGATGAAGAAACAAAGAAAGCAGAAAAAAAGAAATTCATAACCAAAGAACAAGAACCTGAACAATATTGGCAATCAGCAGGAGAGAGAGAAGGAGAGAATCCAATGAAGACACCACTTCCTTACATTATCATATTTGGTATGTCAACTCCTTTTGTAATCTTAGCCATTGCTTTTGCAAATGGTTGGATTAAGGTACCTATTAGATAAGATCAAACAAACCAAACACTTCCTTAAATTATGTTCTGTCCACCAATTTTATTTAAGCACAAGTACTTCTCAAGCTTGAGTAAATAATTTATTTACTTTTTTTTCTTTCATATATGTAATGTACAAACATTATGTATAAATAAATATCTATTNATATGATGAATTAAAA
TARGET=298,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2568
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.233224
PRIMER_LEFT_SEQUENCE=ACCATTCTCTCTCCCAAAACAA
PRIMER_RIGHT_SEQUENCE=GGAAGTGGTGTCTTCATTGGAT
PRIMER_LEFT=164,22
PRIMER_RIGHT=336,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.233
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.3357
PRIMER_LEFT_1_PENALTY=0.102490
PRIMER_RIGHT_1_PENALTY=0.233224
PRIMER_LEFT_1_SEQUENCE=CCATTCTCTCTCCCAAAACAAG
PRIMER_RIGHT_1_SEQUENCE=GGAAGTGGTGTCTTCATTGGAT
PRIMER_LEFT_1=165,22
PRIMER_RIGHT_1=336,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.233
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.4182
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.394672
PRIMER_LEFT_2_SEQUENCE=ACCATTCTCTCTCCCAAAACAA
PRIMER_RIGHT_2_SEQUENCE=AAGGAAGTGGTGTCTTCATTGG
PRIMER_LEFT_2=164,22
PRIMER_RIGHT_2=338,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.395
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=175
PRIMER_PAIR_PENALTY_3=0.4972
PRIMER_LEFT_3_PENALTY=0.102490
PRIMER_RIGHT_3_PENALTY=0.394672
PRIMER_LEFT_3_SEQUENCE=CCATTCTCTCTCCCAAAACAAG
PRIMER_RIGHT_3_SEQUENCE=AAGGAAGTGGTGTCTTCATTGG
PRIMER_LEFT_3=165,22
PRIMER_RIGHT_3=338,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.395
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.7491
PRIMER_LEFT_4_PENALTY=0.515892
PRIMER_RIGHT_4_PENALTY=0.233224
PRIMER_LEFT_4_SEQUENCE=ACACCATTCTCTCTCCCAAAAC
PRIMER_RIGHT_4_SEQUENCE=GGAAGTGGTGTCTTCATTGGAT
PRIMER_LEFT_4=162,22
PRIMER_RIGHT_4=336,22
PRIMER_LEFT_4_TM=59.484
PRIMER_RIGHT_4_TM=60.233
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_03836 Ps_contig_mira-and-tgicl-ass_franssen_31191
SEQUENCE=TCGCAAAAAATTAAACGAATTTGGATAATAATAATAACAATAATTCGAAAAAAAGAAAGAAAGAATATCCTCTCATCTCTATTCTGTGACGAGAACCTCAAGCCTTCAAGCCTGAAGCGTCTCGTACACAAACCTTCTCATCCATGGCGAAGACACTGATTTCTTCTCCATCATTCCTCGGTACTCCACTTCCTTCACTTCACCGTACTTTCTCCCCTAATCGCACCAGGCTTTTCACCAAAGTTCAATTCAGTTTCCACCAACTTCCTCCGATTCAATCCGTAAGTCATTCTGTTGACTTATCCGGAATCTTCGCTAGAGCCGAAGGTTTACTTTACACGCTCGCCGATGCTACTGTTGCGGCGGATGCGGCTGCTTCCACTGATGTTGCTGCGCAGAAGAACGGAGGTTGGTTCGGTTTTATTTCTGATGGAATGGAGTTTGTTCTCAAGGTGTTAAAGGATGGTTTGTCTTCCGTGCACGTGCCTTACTCATATGGATTTGCTATCATATTACTAACTGTTATTGTTAAGGCTGCTACACTTCCCTTGACAAAGCAACAGGTTGAATCAACACTAGCTATGCAAAACCTTCAACCTAAAATTAAGGCCATTCAAGAAAGATATGCTGGCAATCAGGAAAGAATACAACTTGAGACCTCAAGGCTTTATACTCAGGCTGGGGTTAACCCGTTGGCAGGTTGTTTACCAACTTTGGCTACTATTCCAGTCTGGATTGGTCTATACCAAGCTTTATCTAATGTGGCAAATGAGGGACTGTTAACAGAAGGTTTCTTATGGATCCCTTCTCTGGGTGGTCCCACTAGCATTGCTGCTAGACAAAGCGGATCCGGAATTTCTTGGCTTTTTCCGTTTGTGGATGGCCATCCACCTTTGGGTTGGTATGACACTGCAGCATATCTTGTTTTACCTGTTCTTCTTATTGTTTCTCAATATGTTTCAATGGAAATCATGAAACCCCCTCAGACAAATGATCCTAATCAAAAGAATACACTTCTTATTTTCAAGTTTCTCCCACTCATGATCGGCTACTTCTCATTATCTGTCCCATCAGGACTAACAATTTACTGGTTTACAAATAATGTCCTTAGCACAGCTCAACAAGTATGGTTGCGCAAACTAGGAGGTGCAAAGCCCGCTGTTAATGAAAATGCTGGCGGAATTATAACTGCAGGACAAGCTAAGAGATCAGCTTCTAAGCCAGAAAAGGGTGGTGAAAGGTTTAGGCAGTTAAAAGAAGAGGAAAAGAAGAAAAAGCTAATCAAGGCACTCCCAGTGGAGGAAGTTCAGCCTTTGGCTTCTGCTTCTGCGTCCAATGATGGCTCAGATGTAGAAAACAACAAGGAACAAGAAGTTACAGAAGAATCGAACACTTCTAAAGTTAGCCAAGAGGTTCAAAGTTTTTCTAGGGAAAGAAGAAGTAAGCGTTCAAAGCGGAAGCCTGTTGCATGATAGTGGACCACATACATTTGTTTGTAGTTTATAGTAAGTTTTGTATATGTCAAACAGTTTGTATCATTTTTGGGTTGACAATTTTATTGAACATGTTATTTAATCATGCAAAATATCTTTTGTTTCATTTAAGTTCCACATGTTAGCAAAATAAAATTGTCCTTTAACATATTCTTACTTTCTG
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03837 Ps_contig_mira-and-tgicl-ass_franssen_31191
SEQUENCE=TCGCAAAAAATTAAACGAATTTGGATAATAATAATAACAATAATTCGAAAAAAAGAAAGAAAGAATATCCTCTCATCTCTATTCTGTGACGAGAACCTCAAGCCTTCAAGCCTGAAGCGTCTCGTACACAAACCTTCTCATCCATGGCGAAGACACTGATTTCTTCTCCATCATTCCTCGGTACTCCACTTCCTTCACTTCACCGTACTTTCTCCCCTAATCGCACCAGGCTTTTCACCAAAGTTCAATTCAGTTTCCACCAACTTCCTCCGATTCAATCCGTAAGTCATTCTGTTGACTTATCCGGAATCTTCGCTAGAGCCGAAGGTTTACTTTACACGCTCGCCGATGCTACTGTTGCGGCGGATGCGGCTGCTTCCACTGATGTTGCTGCGCAGAAGAACGGAGGTTGGTTCGGTTTTATTTCTGATGGAATGGAGTTTGTTCTCAAGGTGTTAAAGGATGGTTTGTCTTCCGTGCACGTGCCTTACTCATATGGATTTGCTATCATATTACTAACTGTTATTGTTAAGGCTGCTACACTTCCCTTGACAAAGCAACAGGTTGAATCAACACTAGCTATGCAAAACCTTCAACCTAAAATTAAGGCCATTCAAGAAAGATATGCTGGCAATCAGGAAAGAATACAACTTGAGACCTCAAGGCTTTATACTCAGGCTGGGGTTAACCCGTTGGCAGGTTGTTTACCAACTTTGGCTACTATTCCAGTCTGGATTGGTCTATACCAAGCTTTATCTAATGTGGCAAATGAGGGACTGTTAACAGAAGGTTTCTTATGGATCCCTTCTCTGGGTGGTCCCACTAGCATTGCTGCTAGACAAAGCGGATCCGGAATTTCTTGGCTTTTTCCGTTTGTGGATGGCCATCCACCTTTGGGTTGGTATGACACTGCAGCATATCTTGTTTTACCTGTTCTTCTTATTGTTTCTCAATATGTTTCAATGGAAATCATGAAACCCCCTCAGACAAATGATCCTAATCAAAAGAATACACTTCTTATTTTCAAGTTTCTCCCACTCATGATCGGCTACTTCTCATTATCTGTCCCATCAGGACTAACAATTTACTGGTTTACAAATAATGTCCTTAGCACAGCTCAACAAGTATGGTTGCGCAAACTAGGAGGTGCAAAGCCCGCTGTTAATGAAAATGCTGGCGGAATTATAACTGCAGGACAAGCTAAGAGATCAGCTTCTAAGCCAGAAAAGGGTGGTGAAAGGTTTAGGCAGTTAAAAGAAGAGGAAAAGAAGAAAAAGCTAATCAAGGCACTCCCAGTGGAGGAAGTTCAGCCTTTGGCTTCTGCTTCTGCGTCCAATGATGGCTCAGATGTAGAAAACAACAAGGAACAAGAAGTTACAGAAGAATCGAACACTTCTAAAGTTAGCCAAGAGGTTCAAAGTTTTTCTAGGGAAAGAAGAAGTAAGCGTTCAAAGCGGAAGCCTGTTGCATGATAGTGGACCACATACATTTGTTTGTAGTTTATAGTAAGTTTTGTATATGTCAAACAGTTTGTATCATTTTTGGGTTGACAATTTTATTGAACATGTTATTTAATCATGCAAAATATCTTTTGTTTCATTTAAGTTCCACATGTTAGCAAAATAAAATTGTCCTTTAACATATTCTTACTTTCTG
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03838 Ps_contig_mira-and-tgicl-ass_franssen_31196
SEQUENCE=TTCAACGACCAGAGTAGCGGGACGTCACTCAGCTACCAACCAAGCTCTCTCTCTGTCCACTATTTCTACTCAYCCTTATCCTCTCTTTCGGRCGCCACCAATTCACTCACCACAAACAAAATTCATTCACACACACATTTCTCTGAACCTCTCTTCTCTCTCTACTCTACGAAAAGAAAGAAAATAAGAAGAGGATTTTCCTCTTCATCTCTTCATCGCCTCCACCACCCAAAACCACCCTAACAGCCACCATTCACCGCCGAAATCCACCGATTAACCTCCACCCAATAAAACCCCTTCACCAACAACTCCATCGCTTCACCTTCAAATCGCTACCTTCAACCCCTAAATCAACCCGCCACTCACAAATCGAAAACCCTCGCCGCTCAAGCCACCATCGACCAACGACCTTCTGCTCTCTCCGCCGTAGACCGGTCCATCACCATTTCTTCAAGATCCTTCCTATCACCGGTAACCGCAGAATAAAACCCAACGGCGCTTCGGCATTTAGCTCGGATCTAGATTCTCGGTGACTTAAGGATATTTGGGAGTATTTGATTCACAACGGTAGCTTGAAGAAGACAGTGTCGTGACTTATCTTCAAAAAGCTCAAGGTTGGAGTATGGTTTTTCTGTTGTACGCGAACGAAGCAACGCCGAAACCACAATGGTTTGAGGATGTTGAAAGGGTTGGTATTAGCAGTTGATGCGGTCTGCTAAGGATGTAGTAACTCGGTGTAAAAGCAACATCGCTCGCAAGCCAAGGATGCAACAACAACGGTTCTGTTATTTGGTAACTCCTTTGTTCAAGAACTCATCACATTTTTGAGATGTTTTTTTTATTATTTGAATCATTTTATGTTGGAATACGTTGATATTATGGATAGACTTTGTGTGATATATACTGCGTTGTTAACATTGCAACAACCACCTCCAAGAATTTGGTAATCTTTAAATCCTTTGGTTTTGTTTGCATTATAATTGCCATTGTTGTGCATTGGGATTTGCCRTATNRANTTGTTAACTTCTCGTTAATATTCCGGCCTTCGTAACAGAACTTGTTTTGGTTAAATTTATGAGTTAANAAAGTTGTTCGATTTCTTGGCCAATAAAG
TARGET=45,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03839 Ps_contig_mira-and-tgicl-ass_franssen_31196
SEQUENCE=TTCAACGACCAGAGTAGCGGGACGTCACTCAGCTACCAACCAAGCTCTCTCTCTGTCCACTATTTCTACTCAYCCTTATCCTCTCTTTCGGRCGCCACCAATTCACTCACCACAAACAAAATTCATTCACACACACATTTCTCTGAACCTCTCTTCTCTCTCTACTCTACGAAAAGAAAGAAAATAAGAAGAGGATTTTCCTCTTCATCTCTTCATCGCCTCCACCACCCAAAACCACCCTAACAGCCACCATTCACCGCCGAAATCCACCGATTAACCTCCACCCAATAAAACCCCTTCACCAACAACTCCATCGCTTCACCTTCAAATCGCTACCTTCAACCCCTAAATCAACCCGCCACTCACAAATCGAAAACCCTCGCCGCTCAAGCCACCATCGACCAACGACCTTCTGCTCTCTCCGCCGTAGACCGGTCCATCACCATTTCTTCAAGATCCTTCCTATCACCGGTAACCGCAGAATAAAACCCAACGGCGCTTCGGCATTTAGCTCGGATCTAGATTCTCGGTGACTTAAGGATATTTGGGAGTATTTGATTCACAACGGTAGCTTGAAGAAGACAGTGTCGTGACTTATCTTCAAAAAGCTCAAGGTTGGAGTATGGTTTTTCTGTTGTACGCGAACGAAGCAACGCCGAAACCACAATGGTTTGAGGATGTTGAAAGGGTTGGTATTAGCAGTTGATGCGGTCTGCTAAGGATGTAGTAACTCGGTGTAAAAGCAACATCGCTCGCAAGCCAAGGATGCAACAACAACGGTTCTGTTATTTGGTAACTCCTTTGTTCAAGAACTCATCACATTTTTGAGATGTTTTTTTTATTATTTGAATCATTTTATGTTGGAATACGTTGATATTATGGATAGACTTTGTGTGATATATACTGCGTTGTTAACATTGCAACAACCACCTCCAAGAATTTGGTAATCTTTAAATCCTTTGGTTTTGTTTGCATTATAATTGCCATTGTTGTGCATTGGGATTTGCCRTATNRANTTGTTAACTTCTCGTTAATATTCCGGCCTTCGTAACAGAACTTGTTTTGGTTAAATTTATGAGTTAANAAAGTTGTTCGATTTCTTGGCCAATAAAG
TARGET=128,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03840 Ps_contig_mira-and-tgicl-ass_franssen_31201
SEQUENCE=TCAACGCAGAGTACGCGGGATCCATTTTGTTTTGTTATTCATTTTCATTTTCAGAGATCGAGCAATGGCATCTGCAATAGTTCGTAGAAACATCACCCACAACTTGAAAAAGCTCCCGTTTTTATCTTCTTCGCTTTTCAATAATAAGCACTTATTCAACGTTGAACCTTCCGGTTCCGTTTCCTTTCATTCAGAAACCGTTGCTGGTGGTGGTGGACTGCCATCTTACATGCGTGGAGCTGTTTATTGGGAACCTAACAAACCTCTCACCATTGAAGAATTTCACATTCCTCGCCCCAAAGCCGGTGAACTTCTCATCAAAACCAAGAGCATGTGGAGTGTGCCACTCTGATCTTCATGTCATGAAAGCTGAAATTCCGTTTGCAACTCCTTGTGTTGTAGGGCATGAGATTACTGGTGAAGTTATTGAACATGGACAGAACACCGATTCCAAAACCATAGAAAGGCTTCCGGTTGGATCTCGAGTTGTTGGGGCCTTCATCATGCCTTGTGGTAATTGCTCTTATTGTTCAAAGGGCCATGATGATCTGTGTGAAGCTTTCTTTGCTTATAACCGGGCTAAAGGAACCCTCTACGATGGTGAAACTAGACTATTTCTTCGAAGCAGTGGAAAACCGGTATTCATGTATAGTATGGGAGGGCTTGCGGAATACTGTGTTGTGCCAGCAAATGCATTGGCTGTGTTACCAAGCTCAATGCCGTATACTGAGTCTGCAATTCTAGGATGTGCTGTTTTTACTGCTTATGGTGCCATGGCTCATGCGGCTGAAGTGCGTCCGGGTGATTCTGTTGCTGTTATTGGAACTGGGGGTGTTGGTTCTAGTTGTTTGCAGATCGCTAGAGCATTTGGTGCCTCGGATATAATTGCTGTTGATGTTCAGGATAATAAGCTAGAGAAAGCAAAAACACTCGGTGCTACTCACACTATAAATTCGGCAAAGGAAGATCCCATTGAAAAGATACTCGAAATTACTGGTGGAAAAGGCGTTGACGTTGCGGTTGAAGCCTTGGGATTACCACTGACATTTGCTCAGTGCACACAAAGTGTGAAGGATGGAGGAAAAGCAGTGATGATTGGACTGGCAAAAGCTGGTTCTTTAGGAGAGATCGATATAAATAGACTTGTTCGCAGAAAGATAAAAGTAATTGGGTCATATGGAGGCAGGGCTAGACAAGATCTTCCAAAGTTAATTAGATTAGCAGAAACAGGAATCTTCGACCTTGGCCATGCTGTTTCAAGAAAATACACTTTTGAGGAGTCTGGCAAAGCATTCCAGGATCTAAACGAAGGAAAAATCGTCGGCCGAGCTGTAATCGAGATCGTATAGCTCTACGTTCCAGGCAATGCTTTTGTGCAAGACAACCAAGTTTCTGTCTCACGCATTGGAGTGGCAAAGGGGTTGGTGTCCTGAGAACACATATAGGTGTCTTCTATTCTCATGTGTTATCTGTCCTGATAAAATAACTGTATTTGTAAGGACATTTTCAAATAAACACACTTTTTGCTTAATTTTGC
TARGET=205,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0915
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.071684
PRIMER_LEFT_SEQUENCE=CGTTTCCTTTCATTCAGAAACC
PRIMER_RIGHT_SEQUENCE=ATGCCCTACAACACAAGGAGTT
PRIMER_LEFT=177,22
PRIMER_RIGHT=406,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=59.928
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.0915
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.071684
PRIMER_LEFT_1_SEQUENCE=GTTTCCTTTCATTCAGAAACCG
PRIMER_RIGHT_1_SEQUENCE=ATGCCCTACAACACAAGGAGTT
PRIMER_LEFT_1=178,22
PRIMER_RIGHT_1=406,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=229
PRIMER_PAIR_PENALTY_2=0.0915
PRIMER_LEFT_2_PENALTY=0.019805
PRIMER_RIGHT_2_PENALTY=0.071684
PRIMER_LEFT_2_SEQUENCE=CCGTTTCCTTTCATTCAGAAAC
PRIMER_RIGHT_2_SEQUENCE=ATGCCCTACAACACAAGGAGTT
PRIMER_LEFT_2=176,22
PRIMER_RIGHT_2=406,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=59.928
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.1287
PRIMER_LEFT_3_PENALTY=0.019805
PRIMER_RIGHT_3_PENALTY=0.108929
PRIMER_LEFT_3_SEQUENCE=CGTTTCCTTTCATTCAGAAACC
PRIMER_RIGHT_3_SEQUENCE=CTATGGTTTTGGAATCGGTGTT
PRIMER_LEFT_3=177,22
PRIMER_RIGHT_3=461,22
PRIMER_LEFT_3_TM=59.980
PRIMER_RIGHT_3_TM=60.109
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.1287
PRIMER_LEFT_4_PENALTY=0.019805
PRIMER_RIGHT_4_PENALTY=0.108929
PRIMER_LEFT_4_SEQUENCE=CCGTTTCCTTTCATTCAGAAAC
PRIMER_RIGHT_4_SEQUENCE=CTATGGTTTTGGAATCGGTGTT
PRIMER_LEFT_4=176,22
PRIMER_RIGHT_4=461,22
PRIMER_LEFT_4_TM=59.980
PRIMER_RIGHT_4_TM=60.109
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_03841 Ps_contig_mira-and-tgicl-ass_franssen_31203
SEQUENCE=CTTGTGAGTGAGGGAGTGAATCAAAACCAACAAGAAAGAAAGAAAGGAAATACCATTTCTCTTTCTCTTTCTCCTCCCTCACGCAATTNCCTTAACCCTAATTATTCTCCTCTTCCTCAATTTCTCAATTCTTCTTCTTCTTCTTCTTCTAGAGTAACAAACCATTACCATGGCTACTATCTTCCGAGTCGCTTCCTCCACCGCTCCTCCGCCGCATAACTTCCGTCTACGGAAAACTCCCGTTTCTAACGGCTTCTCTTTCTCGTCAATTTGTTTTGACAGGAGAATTGTTAGCTCCGGTGGCCGTAGATTCATTGTTGCTGCTAGTCCTCCAACTGAAGACGCTGTCGTTGCTACTGAACCGCTTACGAAACAGGATCTCATCGATTATCTCGCCTCTGGTTGTAAGCCCAAGGATAAATGGAGAATAGGTACTGAACATGAAAAGTTTGGTTTTGAGCTTGGAAGCCTGCGTCCTGTGAAGTATGAACAAATAGCAGAATTGCTGAATGCCATTGCGGAGAGATTTGACTGGGAAAAAATAATGGAAGGTGATAAGATTATTGGACTCAAACAGGGGAAGCAAAGCATATCATTGGAGCCAGGTGGTCAGTTTGAACTTAGTGGAGCCCCTCTTGAAACCCTGCATCAAACTTGTGCTGAAGTTAATTCACACCTCTATCAAGTAAAAGCGGTTACTGAGGAAATGGGAATTGGATTTCTGGGGATTGGTTTCCAGCCAAAGTGGGAGCGCAAAGACATACCTATGATGCCAAAGGGAAGATATGAGATTATGAAAAAGTACATGCCCAAAGTTGGTTCTCTTGGGCTTGATATGATGTTCAGGACGTGCACTGTACAGGTCAATCTGGACTTTAGTTCTGAAGCTGACATGATCAGGAAATTTCGTGCTGGCCTAGCTTTGCAGCCTATAGCAACAGCTCTTTTTGCAAATTCACCTTTCACAGAGGGAAAGCCCAATGGGTTTGTCAGTATGAGAAGTCATATTTGGACTGATACTGACAAGGACCGCACAGGCATGCTGCCTTTTGTTTTCGACGACTCTTTCGGGTTTGAGCAGTACGTTGATTATGCTCTTGATGTTCCAATGTATTTTGCGTATCGGAAAAAGAAATATGTCGACTGTACTGGAATGACCTTCAGGGACTTTTTGGCAGGAAAGCTTCCTTGTATTCCTGGTGAATTACCAACTCTTAATGATTGGGAGAATCATTTGACCACTATCTTTCCCGAGGTCAGGCTAAAGAGGTATTTGGAGATGAGAGGGGCTGATGGAGGACCTTGGAGAAGGTTGTGTGCTTTACCAGCATTTTGGGTAGGTTTATTGTACGATGAGGTTTCTTTGCAGCGTGTTTTGGATATGACTGCAGATTGGACTCTAGAAGAAAGAGAAATGCTAAGGAATAAGGTCACTGTAACCGGTCTAAAGACACCATTTCGAGATGGTTTGCTGAAGCATGTTGCTGAAGAGGTTCTAGAGTTGGCAAAGGATGGCTTGGAAAGAAGAGGCTTTAAGGAATCAGGATTTTTAAATGCGGTAGCTGAGGTGGTTAGAACAGGTGTGACTCCAGCTGAGAGACTTTTGGAATTGTATCATGGAAAGTGGGAGCAATCTGTAGATCATGTATTTGAGGAATTGCTTTATTAAGGTTCTTTCTGTGAGAGGTTAATTGTGCAAACCACCGCCTTTTTGGGTAGAATCTTGTGTAAAACTTTGGCTATAGTCCTTCTGATTATCTCTCTTGGGATCCGCTTTTAGATATAGGGAATAATATGTGATGTAATTTTTTGCCCTGTAAGGCTTATATACTTGGAAACGTGGTCAGTTCAAACTGGTATTAATTCATGTCAGTCTAATAATTTTCACTGTAGTGTTGTGATATTAATGGTATGTGGTAGATGCAAATGTTGCTAGAACGAACTTTCATTTTCTCTAAATCAAAACTGGATACCAAATTCTATTAATCAATAACAAAGTTTTGTTTTATTTAC
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1542
PRIMER_LEFT_PENALTY=0.151533
PRIMER_RIGHT_PENALTY=0.002623
PRIMER_LEFT_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_RIGHT_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_LEFT=9,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=59.848
PRIMER_RIGHT_TM=59.997
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.2741
PRIMER_LEFT_1_PENALTY=0.151533
PRIMER_RIGHT_1_PENALTY=0.122569
PRIMER_LEFT_1_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_RIGHT_1_SEQUENCE=GGAAGCGACTCGGAAGATAGTA
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=195,22
PRIMER_LEFT_1_TM=59.848
PRIMER_RIGHT_1_TM=59.877
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=4.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.2914
PRIMER_LEFT_2_PENALTY=0.288804
PRIMER_RIGHT_2_PENALTY=0.002623
PRIMER_LEFT_2_SEQUENCE=TGAGTGAGGGAGTGAATCAAAA
PRIMER_RIGHT_2_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=248,22
PRIMER_LEFT_2_TM=59.711
PRIMER_RIGHT_2_TM=59.997
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.2938
PRIMER_LEFT_3_PENALTY=0.151533
PRIMER_RIGHT_3_PENALTY=0.142283
PRIMER_LEFT_3_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_RIGHT_3_SEQUENCE=CGAGAAAGAGAAGCCGTTAGAA
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=264,22
PRIMER_LEFT_3_TM=59.848
PRIMER_RIGHT_3_TM=60.142
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.3836
PRIMER_LEFT_4_PENALTY=0.151533
PRIMER_RIGHT_4_PENALTY=0.232060
PRIMER_LEFT_4_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_RIGHT_4_SEQUENCE=CGACTCGGAAGATAGTAGCCAT
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=190,22
PRIMER_LEFT_4_TM=59.848
PRIMER_RIGHT_4_TM=59.768
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_03842 Ps_contig_mira-and-tgicl-ass_franssen_31203
SEQUENCE=CTTGTGAGTGAGGGAGTGAATCAAAACCAACAAGAAAGAAAGAAAGGAAATACCATTTCTCTTTCTCTTTCTCCTCCCTCACGCAATTNCCTTAACCCTAATTATTCTCCTCTTCCTCAATTTCTCAATTCTTCTTCTTCTTCTTCTTCTAGAGTAACAAACCATTACCATGGCTACTATCTTCCGAGTCGCTTCCTCCACCGCTCCTCCGCCGCATAACTTCCGTCTACGGAAAACTCCCGTTTCTAACGGCTTCTCTTTCTCGTCAATTTGTTTTGACAGGAGAATTGTTAGCTCCGGTGGCCGTAGATTCATTGTTGCTGCTAGTCCTCCAACTGAAGACGCTGTCGTTGCTACTGAACCGCTTACGAAACAGGATCTCATCGATTATCTCGCCTCTGGTTGTAAGCCCAAGGATAAATGGAGAATAGGTACTGAACATGAAAAGTTTGGTTTTGAGCTTGGAAGCCTGCGTCCTGTGAAGTATGAACAAATAGCAGAATTGCTGAATGCCATTGCGGAGAGATTTGACTGGGAAAAAATAATGGAAGGTGATAAGATTATTGGACTCAAACAGGGGAAGCAAAGCATATCATTGGAGCCAGGTGGTCAGTTTGAACTTAGTGGAGCCCCTCTTGAAACCCTGCATCAAACTTGTGCTGAAGTTAATTCACACCTCTATCAAGTAAAAGCGGTTACTGAGGAAATGGGAATTGGATTTCTGGGGATTGGTTTCCAGCCAAAGTGGGAGCGCAAAGACATACCTATGATGCCAAAGGGAAGATATGAGATTATGAAAAAGTACATGCCCAAAGTTGGTTCTCTTGGGCTTGATATGATGTTCAGGACGTGCACTGTACAGGTCAATCTGGACTTTAGTTCTGAAGCTGACATGATCAGGAAATTTCGTGCTGGCCTAGCTTTGCAGCCTATAGCAACAGCTCTTTTTGCAAATTCACCTTTCACAGAGGGAAAGCCCAATGGGTTTGTCAGTATGAGAAGTCATATTTGGACTGATACTGACAAGGACCGCACAGGCATGCTGCCTTTTGTTTTCGACGACTCTTTCGGGTTTGAGCAGTACGTTGATTATGCTCTTGATGTTCCAATGTATTTTGCGTATCGGAAAAAGAAATATGTCGACTGTACTGGAATGACCTTCAGGGACTTTTTGGCAGGAAAGCTTCCTTGTATTCCTGGTGAATTACCAACTCTTAATGATTGGGAGAATCATTTGACCACTATCTTTCCCGAGGTCAGGCTAAAGAGGTATTTGGAGATGAGAGGGGCTGATGGAGGACCTTGGAGAAGGTTGTGTGCTTTACCAGCATTTTGGGTAGGTTTATTGTACGATGAGGTTTCTTTGCAGCGTGTTTTGGATATGACTGCAGATTGGACTCTAGAAGAAAGAGAAATGCTAAGGAATAAGGTCACTGTAACCGGTCTAAAGACACCATTTCGAGATGGTTTGCTGAAGCATGTTGCTGAAGAGGTTCTAGAGTTGGCAAAGGATGGCTTGGAAAGAAGAGGCTTTAAGGAATCAGGATTTTTAAATGCGGTAGCTGAGGTGGTTAGAACAGGTGTGACTCCAGCTGAGAGACTTTTGGAATTGTATCATGGAAAGTGGGAGCAATCTGTAGATCATGTATTTGAGGAATTGCTTTATTAAGGTTCTTTCTGTGAGAGGTTAATTGTGCAAACCACCGCCTTTTTGGGTAGAATCTTGTGTAAAACTTTGGCTATAGTCCTTCTGATTATCTCTCTTGGGATCCGCTTTTAGATATAGGGAATAATATGTGATGTAATTTTTTGCCCTGTAAGGCTTATATACTTGGAAACGTGGTCAGTTCAAACTGGTATTAATTCATGTCAGTCTAATAATTTTCACTGTAGTGTTGTGATATTAATGGTATGTGGTAGATGCAAATGTTGCTAGAACGAACTTTCATTTTCTCTAAATCAAAACTGGATACCAAATTCTATTAATCAATAACAAAGTTTTGTTTTATTTAC
TARGET=129,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1542
PRIMER_LEFT_PENALTY=0.151533
PRIMER_RIGHT_PENALTY=0.002623
PRIMER_LEFT_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_RIGHT_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_LEFT=9,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=59.848
PRIMER_RIGHT_TM=59.997
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.2741
PRIMER_LEFT_1_PENALTY=0.151533
PRIMER_RIGHT_1_PENALTY=0.122569
PRIMER_LEFT_1_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_RIGHT_1_SEQUENCE=GGAAGCGACTCGGAAGATAGTA
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=195,22
PRIMER_LEFT_1_TM=59.848
PRIMER_RIGHT_1_TM=59.877
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=4.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.2914
PRIMER_LEFT_2_PENALTY=0.288804
PRIMER_RIGHT_2_PENALTY=0.002623
PRIMER_LEFT_2_SEQUENCE=TGAGTGAGGGAGTGAATCAAAA
PRIMER_RIGHT_2_SEQUENCE=TTAGAAACGGGAGTTTTCCGTA
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=248,22
PRIMER_LEFT_2_TM=59.711
PRIMER_RIGHT_2_TM=59.997
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.2938
PRIMER_LEFT_3_PENALTY=0.151533
PRIMER_RIGHT_3_PENALTY=0.142283
PRIMER_LEFT_3_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_RIGHT_3_SEQUENCE=CGAGAAAGAGAAGCCGTTAGAA
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=264,22
PRIMER_LEFT_3_TM=59.848
PRIMER_RIGHT_3_TM=60.142
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.3836
PRIMER_LEFT_4_PENALTY=0.151533
PRIMER_RIGHT_4_PENALTY=0.232060
PRIMER_LEFT_4_SEQUENCE=GAGGGAGTGAATCAAAACCAAC
PRIMER_RIGHT_4_SEQUENCE=CGACTCGGAAGATAGTAGCCAT
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=190,22
PRIMER_LEFT_4_TM=59.848
PRIMER_RIGHT_4_TM=59.768
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_03843 Ps_contig_mira-and-tgicl-ass_franssen_31205
SEQUENCE=AGRAACTGTCAAAACCAACGAACAACCTATCCCATTACACCAAGCAATCAAAGGCTCTCTCTATCTATAATCGCGGCAAAAACACGAAAGCATCGCACTTGGCAAAGATATCAAAAAAGAAAAGAAAGAAAGAGACATAAACGGCTTTTTTCGCGCAAATGTGTGAGATACAGCTTCTTCGCTGTATTCTCATTCCCATGCCTCTTTGCCTCGATAGCTTCCTCCGTTAGATTGGATCTTCACCATGGAAAACATTGCCGCGCAATTGAAGCGCGGCATCTCGCGCCAATTCTCCTCCGGTTCGTTAAGGAAGACGTTAAGCCGGCAATTCACACGTCAGGCTTCGCTCGATCCGCGCCGGAATAATCTCCGCTTTAGCTTCGGTCGTCAATCGTCGCTTGATCCGATCCGGCGAAGTACCAGCGAAGATCAGCCTGAACTCACCGTGCCGGAGAATCTTGACTCCACCATGCAGCTTCTCTTTATGGCTTGCCGTGGTGACGTTAAAGGTGTGGAGGATTTGCTTAACGAAGGCATCGATGTTAATAGCATTGATCTCGATGGTCGTACTGCTCTACATATCGCCGCCTGTGAAGGTCACGTCGACGTTGCTAAATTATTGCTCAGCCGTAGGGCTAACCTCGACGCTCGTGATCGTTGGGGCAGTACGGCAGCTGCCGATGCTAAATATTATGGAAATACAGAAGTTTATTATATGTTGAAGGCGCGTGGAGCAAAAGTGCCGAAAACCAGGAAGACTCCGATGACTGTTGCAAATCCTCGTGAAGTTCCCGAATATGAACTCAATCCATTAGAACTACAGGTTCGGAAGAGTGATGGTATTTCAACGGGGACATATCAAGTAGCTAAATGGAATGGCACAAAGGTTGCAGTGAAGATACTTGATAAAGACAGTTATTCAAATCCTGATACAATAAATGTTTTCAAACATGAGCTCACATTATTGGAAAGGGTGCGTCATCCTAATGTGGTCCAATTTGTTGGAGCTGTCACCCAAAACATACCTATGATGATTGTTCGAGAGTACCATGCTAAGGGTGACTTAACAGGTTATATTCAAAAGAAAGGGCGTTTGTCCCCATCAAAAGTTCTACGGTTTTCTCTTGATATTGCTAGGGGCATGAATTATCTTCACGAATGCAAACCAGAGCCTGTTATCCACTGTGATTTAAGGCCAAAGTGCCAGAAATATTTTGCTGGATAATGGAGGGCAATTGAAGGTAGCTGGATTTGGTACTGTGAGGTTTTCACTCATCTCACCTGACAAAGCAAAGCTAGTACAGCCCGAGGCCAACATTGACCCTTCAAGCTTATACGTGGCACCGGAGATTTACAATGGTGATGTGTTTGATAGAAGTGTGGATGCATATGCTTTTGGTCTCGTTGTTTATGAGATGATAGAGGGTGTACCACCCTTCCATCCCAAGCCCCCAGAGGAAGCTTTGAAACTGATGTGTTTAGAAGGGAAGAGACCGCAATTCAAAATCAAAACAAAAAGTTATCCTCCCGATTTGAAAGAGTTGATTGAGGAATGTTGGGATCCAGAACCTCTTGTCAGACCAACTTTTTCTCAGGTTATTGCCCGGTTGGACAGAATTGTTGCAAATTGCTCAAAACAAGGATGGTGGAAAGATACTTTTAAACTTCCTTGGAAATAAATAAAATGGGATGCACCATTAGACCAAGGAGGAGATAAAGATCAGAGTTGTATCATCAAAACTGATTTGTGAGCATCATGTTGTTGTAATGTTAATAGCCATTGCAATTAATAACTTCTGAACTCAAATAGTGTGAGGTTGAGTTTGGATTTGAGCACAAAAATGTATTCCACAGCTCCATTCCAACATCGTTCTCTATCTACTAGAGGATCTGAAACAGATTATCTCTGTAAAATGTATCATAAACCACACATGTTTTAGAACATCTATTCTAAGAAATTTTACC
TARGET=121,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03844 Ps_contig_mira-and-tgicl-ass_franssen_31209
SEQUENCE=TTTTAACAACAACAATACTCCTCCTCATGCAATTACACAGCTCACACAGAATAATTATGTATGTATATTATTGAGCATAAATCATCAAATTTTTCTCCTAGATCTCCAGATCCACCTCAGATCAATAATTATAAACCTAACTATCATATTATATCATAAACAATATAACATAAAAAAATATAGTAAAAAAAGAGAGAGAAATTATACATATAAAAAAGACTAAGTAAAATAAAGTTTACATTTTTATGAAACCCAATAACGAAAACCAGATTCCAGAACAAACCCTCACAGAAACAAGATCAAAAATCAATTAATCTGATCGCATCTTCTTCTAATCTCACCTCTTCTACCAGTTTTCACACCCAAAAGACTAAGCTTCTGCATTGAAGAAGCAAAAACCTTAAAGAATTTATCTTCATCCGCAGCAAAAGTTTCCACAAAAGGTTTCGTTAATGGGTCACTGAACAAACCATGATCTGATTTCAAAACACCTAAACCTTTTGGAAGATTCTGAAAATAAACATTATCGAACTTGTTCGGTGTCATAACATCGTTAAAAATCGATAAAGTTGGATTCTTCTGGTAATCACGGCAAGCATTTCGTAAAGCTTCGGCAAAACGAGGATTGTATGAACTTGGTGAGCTAGGAGAGGATGAATTGTTGTATATTTCGGAAGAGAATTCTGAACAATGAGAGAATCCGACTGTGTGAGCACCGGAAAGTGCTACCATTTCTTCGACGGTGAAGCCACGTTTAGTGAAGATTTCGATGATTTGTGACATTGGCATTGATGGTTTAGGGAGATGGCCGTTAACGGAGGGGGAAGCGGAGGTTCGGCCGTCGCGGCGGCCGAGGTAGACGTTGTAGTGGGGACCACCGAGCATGATTAAGAGGTCGCGTGTGGCGGTGGAGAGGATGTCGGAGCAAGAGACGGTGTTGGGACATGAGAATTCGAGGGCGGTTTTGATGCGTACGATGAGGTCGAAAGCGTCGCCGGGGAGAGAGAGGTTGATGTCGTTGTCGCGCTCGGCTTTGTTGGAAGGGGGTCGAGGAGAGGAGGACGGAAGCGTCGCAGCCGTTAGGGAGGAGGCAGTCGTGGAGGAAGAGACGGAGTGTGGCGGCGGCGGTTGTTGGTGATTGGATCTGTTTTGAGGTTACTGTTTGTTGGACGATTTGTTCGAATTGTGGACATGTTTGTTTGTAGTAGTCTAAGGTGAGTCTTGATTCGGAGAGATTAAGGAAAAAGAAGAAGAAGAAGAAGAAGAATGGCAACAACGTTGTTGATGTTGTTTTTGTTGCTGCCATTGTTGTGGTTGTTTTTGTTTTTGGGGTTTGAAGAGTCAAGAAGAG
TARGET=1245,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3000
PRIMER_LEFT_PENALTY=0.029253
PRIMER_RIGHT_PENALTY=0.270774
PRIMER_LEFT_SEQUENCE=TGGTGATTGGATCTGTTTTGAG
PRIMER_RIGHT_SEQUENCE=TCTTCTTGACTCTTCAAACCCC
PRIMER_LEFT=1132,22
PRIMER_RIGHT=1349,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=59.729
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=10.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=0.3376
PRIMER_LEFT_1_PENALTY=0.066849
PRIMER_RIGHT_1_PENALTY=0.270774
PRIMER_LEFT_1_SEQUENCE=TGAGGTTACTGTTTGTTGGACG
PRIMER_RIGHT_1_SEQUENCE=TCTTCTTGACTCTTCAAACCCC
PRIMER_LEFT_1=1150,22
PRIMER_RIGHT_1=1349,22
PRIMER_LEFT_1_TM=60.067
PRIMER_RIGHT_1_TM=59.729
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=10.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.3924
PRIMER_LEFT_2_PENALTY=0.121644
PRIMER_RIGHT_2_PENALTY=0.270774
PRIMER_LEFT_2_SEQUENCE=AGGTGAGTCTTGATTCGGAGAG
PRIMER_RIGHT_2_SEQUENCE=TCTTCTTGACTCTTCAAACCCC
PRIMER_LEFT_2=1212,22
PRIMER_RIGHT_2=1349,22
PRIMER_LEFT_2_TM=59.878
PRIMER_RIGHT_2_TM=59.729
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=10.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=138
PRIMER_PAIR_PENALTY_3=0.4863
PRIMER_LEFT_3_PENALTY=0.121644
PRIMER_RIGHT_3_PENALTY=0.364634
PRIMER_LEFT_3_SEQUENCE=AGGTGAGTCTTGATTCGGAGAG
PRIMER_RIGHT_3_SEQUENCE=TGACTCTTCAAACCCCAAAAAC
PRIMER_LEFT_3=1212,22
PRIMER_RIGHT_3=1343,22
PRIMER_LEFT_3_TM=59.878
PRIMER_RIGHT_3_TM=60.365
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=0.4863
PRIMER_LEFT_4_PENALTY=0.121644
PRIMER_RIGHT_4_PENALTY=0.364634
PRIMER_LEFT_4_SEQUENCE=AGGTGAGTCTTGATTCGGAGAG
PRIMER_RIGHT_4_SEQUENCE=GACTCTTCAAACCCCAAAAACA
PRIMER_LEFT_4=1212,22
PRIMER_RIGHT_4=1342,22
PRIMER_LEFT_4_TM=59.878
PRIMER_RIGHT_4_TM=60.365
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=131
=
PRIMER_SEQUENCE_ID=SSR_03845 Ps_contig_mira-and-tgicl-ass_franssen_31211
SEQUENCE=CGTCGGGGATCAAACACAACTCACTCTCAAAAATCTCTTCAACCAACACCAAGTAGAGAGAATGATGAAGATGAAAGGTATGTCTGCAATGATGATATTTGCGCTGGTGCTCATGTTTGAGGCTGTTTCCATTCTTGAGGCTGCGAACTGTGACCCAATGCAATTAAGCCCATGTTTAGGAGCAATCATGTCAAACACCAAACCTTCTCCTGGTTGTTGCAGCAGGTTGAATGATCAAAAGCCATGCTTGTGTCAATACGTCAGAAACCCTAACTTGAAACAGTATGTTAACTCTCCTGGTTCTAGAAATGTTGCTAGTTCTTGTGGTGTTACCATTCCAAACTGTTAAGAACAACCCTTAACTATATCTATGATCCATCCATCCATGAGGTATTCTATTCTTAAATACAAATAATGTCATTTGAGTTTGTAAGATTAAAAAGCTTGATGTATTGTAACTTCAAAGTTGTGATATGTATTAAGAATGATAAATAAGAAAGTGGTGGTGTTATTATTATGTATATTCAGAGTTATATTAATTTGCTCGAACAAATTAATTATTACTCTGTTATTTATATATATTAATTTGCTCGAACAAATCAAAGTTGTGAGTACAAGTTTGTGTTTATGAAATTTTT
TARGET=374,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03846 Ps_contig_mira-and-tgicl-ass_franssen_31223
SEQUENCE=CGACGTATAGAAGCCACGTGGTGTTGAAAAGTAATGAAAAATGGAAACATTGGTTCAGCTGTCGACACCACGTCCTATATATGGAACCATCATCTTTCGTCTTTATCTCTCTCTAAACTAAACTAAACTAAACTCTCCTATTCTATTTTATTCTATCTTTCAAAAATGGGCACTGCTACCTGCATCGATATTATCCTTGCCATCATCCTTCCACCTCTCGGTGTCTTCCTCAAGTTTGGCTGCAACGTTGAGTTCTGGATCTGTTTGATTCTCACCATTTTGGGCTATCTTCCAGGAATTATCTATGCAATTTATGCTATCACTAAGTAATCTATATCTATCTACAACCTTTTGTGAATCGTGAATAGTTTTCAAGGGTGACATTCAAGTTTCGTGTAAAATTGTTCACTGCGATGGATAAAAGTCATTCTTTTCTATTTTATAGTGTATCTTTTTAGTGTCAATATTCCTTTACTTCTTTTTGGTTTTCTGTTTTTAACTTGAGTTTTTATCATGTGTGGAATTGATAGTGTGAAGCTTGTGAACTCTAAAAATGGCAGCCCATTTGAATTATAGTACTATACCGTCTTAATAATTGTTCATCAATCTTTGACTTTGATATTAATATAATATTTATTCAATGTAATCAAATTAA
TARGET=113,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2013
PRIMER_LEFT_PENALTY=0.167242
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=ATGGAACCATCATCTTTCGTCT
PRIMER_RIGHT_SEQUENCE=AGATAGCCCAAAATGGTGAGAA
PRIMER_LEFT=80,22
PRIMER_RIGHT=289,22
PRIMER_LEFT_TM=59.833
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.2377
PRIMER_LEFT_1_PENALTY=0.167242
PRIMER_RIGHT_1_PENALTY=0.070460
PRIMER_LEFT_1_SEQUENCE=ATGGAACCATCATCTTTCGTCT
PRIMER_RIGHT_1_SEQUENCE=TAATTCCTGGAAGATAGCCCAA
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=300,22
PRIMER_LEFT_1_TM=59.833
PRIMER_RIGHT_1_TM=59.930
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.2511
PRIMER_LEFT_2_PENALTY=0.167242
PRIMER_RIGHT_2_PENALTY=0.083883
PRIMER_LEFT_2_SEQUENCE=ATGGAACCATCATCTTTCGTCT
PRIMER_RIGHT_2_SEQUENCE=AACTTGAGGAAGACACCGAGAG
PRIMER_LEFT_2=80,22
PRIMER_RIGHT_2=235,22
PRIMER_LEFT_2_TM=59.833
PRIMER_RIGHT_2_TM=59.916
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.3932
PRIMER_LEFT_3_PENALTY=0.167242
PRIMER_RIGHT_3_PENALTY=0.225996
PRIMER_LEFT_3_SEQUENCE=ATGGAACCATCATCTTTCGTCT
PRIMER_RIGHT_3_SEQUENCE=CAAACAGATCCAGAACTCAACG
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=59.833
PRIMER_RIGHT_3_TM=59.774
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.4483
PRIMER_LEFT_4_PENALTY=0.167242
PRIMER_RIGHT_4_PENALTY=0.281044
PRIMER_LEFT_4_SEQUENCE=ATGGAACCATCATCTTTCGTCT
PRIMER_RIGHT_4_SEQUENCE=AAACTTGAGGAAGACACCGAGA
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=236,22
PRIMER_LEFT_4_TM=59.833
PRIMER_RIGHT_4_TM=60.281
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_03847 Ps_contig_mira-and-tgicl-ass_franssen_31233
SEQUENCE=AAGCAACAATAAGCTTTAACCATTTATCACTCCACACTCACAGCTTTTTCTNACCGCGCTTATTTCCTCATCTTCCTTACGTAAATTCTCCTTTAAGCAACAAAAAAAATATCCAAAACTTTCTATTCTCACTTTTCATTTCATAACATAACATAACATAACATAACCACTTCCTCATTTTTTTTCCTTGTACTCTGTTTCAAACATAAACATGGTACTCTCTCTTCTCTTCCTAGTCACAATCTTAACCATGACAACCTCAGGAATTTCCGACACTAACTCCTTCATCTTCGGTGGTTGTTCTCGTCTCAAATTCTCACCTGGTTCCAACTACGAAACAAGCGTAAACTCGATCCTCTCCTCACTAGTTAACTCGGCAACGTTCACAACCTACAACAATTTCACTGTTCCGGGAGCCACCGCGTCGGAAACCGTCTCCGGACTTTTTCAGTGCCGCGGGGATATTACCAATGATCAGTGTTCAACCTGCGTGGAGCGTGCGGTGAGTCAGCTCGGAACACTCTGTTTCGAAACCTACGGTGGCGCGTTGCAGCTCGAAGGTTGTTTCGTGAAGTACGATAATGTTAAGTTTGTTGGGGTAGAGGACAAGACGGAAGTGGTCAAGAAATGTGGACCGTTGATTGGGTTGACTTCTGATGCAAGGGATAATGTGCTGGCTTATTTAGAGTCGTCCGATGGGGTTTATAAAACTTTTAGAACTACTTCTTATGGGGATTTTCAGGGTGTGGCGCAGTGCACGGGGGATTTGAGTTCCAGCGAGTGTCAGGATTGTGTTTCCGATGCGATCCAACGGTTGAAAAGTGAGTGTGGAGGTTCTACTTGGGGTGATATGTATTTGGCTAAATGCTATGTGCGGTATTCGGAAGGTGGAGTTCACTCGCGGGGCCATAACGATGATGAAAATCACAATGATGATGAGATTGAGAAGACACTAGCCATACTAATTGGACTCATTGCTGGTGTTGCATTAATAATAGTTTTTCTTTCCTTCTTGTCCAAAGTTTGTGAAAAGCAAAGAGGTGGTAAATGAAGAGTGTGTTATTATGTGGATAGTAATAGTATTGAACTTTTGTCTTTGTCTATTTTATTTGTATTCAATCTTTTCTAAGTTTCCACCATAATTCCCTTTGCCATTGAAGGTGTATAATTATATGTAGAAAAATCTTGTGGAAAAATAGTTTTCTTTACCATTACAATAAGGTCAAAATCACGTGAAAAACACGGTGAGACAGAAGCTATACATATTAGTTTTTCAAAATTACAGTGAAAACGACCTTAAACATGTGACAGTGGTGATCTATCGTACTCGTACGATCGAACATAAGTTCAGATTGGTGCAGATTCCACGTGCCTTTATTTATTTGTTAAGTGTCCATCAATGTGTACAAATAGTGATCATTTAAGTGGAAAATGCATCATCCTATGTTATATAGCCTTTAATGAATGTTAATTAGAGGTTTTTCTTATATATTAGAACATGATGAGTTAACCTTTTACTTTTCATTACAAAAAAAGTTAAATTAAGACAAGTTTGATCACTATTTTCTCGCCCAAATTCTATGTTCCTAACTAAAGTTATCATTTTGNGACTT
TARGET=142,25
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0491
PRIMER_LEFT_PENALTY=0.046801
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=TCACTCCACACTCACAGCTTTT
PRIMER_RIGHT_SEQUENCE=ACCACCGAAGATGAAGGAGTTA
PRIMER_LEFT=26,22
PRIMER_RIGHT=297,22
PRIMER_LEFT_TM=59.953
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.0491
PRIMER_LEFT_1_PENALTY=0.046801
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=TCACTCCACACTCACAGCTTTT
PRIMER_RIGHT_1_SEQUENCE=TAGTGTCGGAAATTCCTGAGGT
PRIMER_LEFT_1=26,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=59.953
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.1701
PRIMER_LEFT_2_PENALTY=0.046801
PRIMER_RIGHT_2_PENALTY=0.123278
PRIMER_LEFT_2_SEQUENCE=TCACTCCACACTCACAGCTTTT
PRIMER_RIGHT_2_SEQUENCE=CCACCGAAGATGAAGGAGTTAG
PRIMER_LEFT_2=26,22
PRIMER_RIGHT_2=296,22
PRIMER_LEFT_2_TM=59.953
PRIMER_RIGHT_2_TM=60.123
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=0.3119
PRIMER_LEFT_3_PENALTY=0.046801
PRIMER_RIGHT_3_PENALTY=0.265053
PRIMER_LEFT_3_SEQUENCE=TCACTCCACACTCACAGCTTTT
PRIMER_RIGHT_3_SEQUENCE=AATTCCTGAGGTTGTCATGGTT
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=267,22
PRIMER_LEFT_3_TM=59.953
PRIMER_RIGHT_3_TM=59.735
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.3119
PRIMER_LEFT_4_PENALTY=0.046801
PRIMER_RIGHT_4_PENALTY=0.265053
PRIMER_LEFT_4_SEQUENCE=TCACTCCACACTCACAGCTTTT
PRIMER_RIGHT_4_SEQUENCE=AAATTCCTGAGGTTGTCATGGT
PRIMER_LEFT_4=26,22
PRIMER_RIGHT_4=268,22
PRIMER_LEFT_4_TM=59.953
PRIMER_RIGHT_4_TM=59.735
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_03848 Ps_contig_mira-and-tgicl-ass_franssen_31242
SEQUENCE=GGTAGTCTAACGCACGAGACTTCTTCTACTCATAGGTTTGTTTTAGGTTTTTTAGTTTCTATGTAAATTTACTACGATCTGGCTCTGTTGTTATTGTTGTTGTTGTTGTAGTTGAGAGATGATCTTCAAGCTTTCGCGAAAGTGTTGGTGGTTGGGGCAGGTGGGTTAGGTTGCGAATTGTTGAAAGATTTAGCCTTAACTGGTTTTCGAAACCTTGAGGTTATTGATATGGATCGTATAGAAGTTACTAATCTTAATCGCCAATTTTTGTTTAGACTTGAGGATGTTGGGAAACCGAAAGCTGAGGTAGCTGCCAAGCGTGTAATGGAAAGAATTAGTGGGGTGGATATTGTGCCACATTTTTGCAGGATTGAGGATAAGGATATTGAGTTTTATAATGATTTTAGTATCATTGCACTTGGTCTTGATTCTATTGAGGCTCGGAGCTACATCAATAATGTTGCTTGTAGTTTTCTAGAGTATGATTCTGATGACAATCCACGGGAAGAGACCATCAAACCTATGGTGGATGGCGGGACTGAAGGTTTCAAGGGCCATGCCAGGGTTATAATGCCAGGGATCACACCGTGCTTTGAGTGTACCATATGGCTTTTTCCACCTCAAGTGAAGTTTCCCTTATGTACACTGGCAGAAACCCCGAGAACTGCTGCCCACTGTATTGAATACGCTCACTTAATTAAATGGGATGAGGTTCATCGTGGAGTTCCTTTTGACCCTGATAACCCCAAGAATATGAAATGGGTCTATGACGAGGCTGTCAAAAGGGCCGAGCTTTTTGGCATTCCTGGAGTTACTTATTCTTTCACCCAGGGTGTTGTGAAGAACATCATACCAGCTATAGCTTCAACAAATGCAATTATATCAGCTGCATGTGCACTGGAAACATTGAAAATTGCAACAGAGTGCAGCAAAACTTTGTCAAACTATTTAACGTATAATGGTTCAGAAGGCCTCCACACTAAGGTGACAGAATTTGAAAGGGACAGAGACTGCCTTGTGTGTGGTCCTGGTATACGTCTTGAACTGGACCCTTCAATCACATTGCAAAAGTTCATGGATCTTCTAGAAGAACATCCCAAATTGCAATTGTCGAAAGCTAGTGTTACACATCGAGGAAAGAATCTGTATATGCAAGCTCCACCTGTATTGGAAGAAATGACTAGATCGAACCTCTCTCTATCTCTTTTTAACCTCATGGGTAAATTGCCCAAAGACATTGTGCACGTGAATGGTATGACAAGCAAGAATGACCAAAAAACTTCATGTTTGAGAAAATTGCGCGTTGTTTTCAAAGGAGTTGATGGAGTTACAGATATGGATACCGCAGGAGGAGCATAACTGAATATTTTCTGGCTTTGTAAGCATTAGTTAATGTTAGATATCATAGCTTGTTTCCAATATTAGTGAGGCAAGATTAACAATTTTTACGATGTAAACGTTTCTCATGGATGATCATCAAATGACATCTGATGCTGCAACTGATTCGGCAAAATTCCATTGTTACGGGCAGTTTGATTGATGGAGCACTAAATTTCATTGTTATGTGCAGTTTGATTGATGGAGCACATGGATATATCATTTGGAATCATGATTCTAGAAATATTTCTCACTGCTACTTTGTTTATTAAACAGAATATTAATCAACCAAAAGTAAAATTATTTTCGTAGTAGGGTAGG
TARGET=94,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6967
PRIMER_LEFT_PENALTY=0.180765
PRIMER_RIGHT_PENALTY=0.515892
PRIMER_LEFT_SEQUENCE=CGCACGAGACTTCTTCTACTCA
PRIMER_RIGHT_SEQUENCE=GTTTCCCAACATCCTCAAGTCT
PRIMER_LEFT=10,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.819
PRIMER_RIGHT_TM=59.484
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=1.1918
PRIMER_LEFT_1_PENALTY=0.180765
PRIMER_RIGHT_1_PENALTY=1.010990
PRIMER_LEFT_1_SEQUENCE=CGCACGAGACTTCTTCTACTCA
PRIMER_RIGHT_1_SEQUENCE=TCAACAATTCGCAACCTAACC
PRIMER_LEFT_1=10,22
PRIMER_RIGHT_1=183,21
PRIMER_LEFT_1_TM=59.819
PRIMER_RIGHT_1_TM=59.989
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=174
PRIMER_PAIR_PENALTY_2=1.3137
PRIMER_LEFT_2_PENALTY=0.180765
PRIMER_RIGHT_2_PENALTY=1.132937
PRIMER_LEFT_2_SEQUENCE=CGCACGAGACTTCTTCTACTCA
PRIMER_RIGHT_2_SEQUENCE=GTTTCGAAAACCAGTTAAGGCT
PRIMER_LEFT_2=10,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=59.819
PRIMER_RIGHT_2_TM=58.867
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=1.3479
PRIMER_LEFT_3_PENALTY=0.180765
PRIMER_RIGHT_3_PENALTY=1.167170
PRIMER_LEFT_3_SEQUENCE=CGCACGAGACTTCTTCTACTCA
PRIMER_RIGHT_3_SEQUENCE=GGTTTCGAAAACCAGTTAAGGC
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=213,22
PRIMER_LEFT_3_TM=59.819
PRIMER_RIGHT_3_TM=61.167
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=1.3968
PRIMER_LEFT_4_PENALTY=0.180765
PRIMER_RIGHT_4_PENALTY=1.216012
PRIMER_LEFT_4_SEQUENCE=CGCACGAGACTTCTTCTACTCA
PRIMER_RIGHT_4_SEQUENCE=TTCAACAATTCGCAACCTAACC
PRIMER_LEFT_4=10,22
PRIMER_RIGHT_4=184,22
PRIMER_LEFT_4_TM=59.819
PRIMER_RIGHT_4_TM=61.216
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_03849 Ps_contig_mira-and-tgicl-ass_franssen_31246
SEQUENCE=TAAAAAACCCCCAAAAACGGGTTACGAACCAATTTTGTAGGGGGAAAATAGCGAGGAATACAAGGAAAATGATGCTGTCTTGTTCTTTGATGGTCAGACACTTCGATTGGAGCGGCTTGATAGGGCTGTAAAGCAACTGCGCCACCTTCGAATGCCTGGTGAGTCTTCAGCTGCTGCATCTGGAACTGTTACTGCTCCATCTGGACCAGCTTTGGATCCTTGGTCACCCCCCATTGGGAAGAACACAAAACCAGCATATTTTGGCAGTGCCAGGAGCTCGAATCAAGCTGTTGCAGTTGAAGTGGAACGTATTGATATTGGTGAACCTGAAAATACTGGCATCAAGTCTGATTCTAAGAGGTCATATGATCAACTAAATGAACCGCCCACTGTTTCTGCTGCCTCACCCGCTGCCATAGATGAAGTTGGGGAACATAGAGATATAGACATTGATGACCTTTTTGGAAGTGGAACACCTGAGGATGACAATAATGTTGAAGAAAAGGATAATGTTGGATTTGACATGAATGTTCCCATCACTGATGATGAGATCGCTGATGTGGATGATAGTGGTGACGAGGTGGACAAAGGACCCAATGCCGCCGAAGCTCTCAGAGCCCAAGTGAATGCAGAGGGGAAGGGTGAAGAAACATCTACTTCTAGCAGTAGCAGTGACAGTGGAAGCAGCAGTGATAGTGGTAGTGGGAGCAGCAGCAGCAGTGATAGTGGAGGCAGCGATGACGACTCAGTTACCTCAATATAAAATTGCACAACTATGATTTTTCTTTCTTTTCCTTTTATCAGTTATCATTGCAACTAATCCAATATATAGAATGTGTTTATTAAGCCCGGGCGAGAAGAATGTAGATTCTAAAAGGTTATTAATGATGTGGAATATGTAGAT
TARGET=707,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3279
PRIMER_LEFT_PENALTY=0.015375
PRIMER_RIGHT_PENALTY=0.312496
PRIMER_LEFT_SEQUENCE=ATCGCTGATGTGGATGATAGTG
PRIMER_RIGHT_SEQUENCE=TGAGGTAACTGAGTCGTCATCG
PRIMER_LEFT=550,22
PRIMER_RIGHT=756,22
PRIMER_LEFT_TM=59.985
PRIMER_RIGHT_TM=60.312
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.3461
PRIMER_LEFT_1_PENALTY=0.015375
PRIMER_RIGHT_1_PENALTY=0.330697
PRIMER_LEFT_1_SEQUENCE=ATCGCTGATGTGGATGATAGTG
PRIMER_RIGHT_1_SEQUENCE=AGGTAACTGAGTCGTCATCGCT
PRIMER_LEFT_1=550,22
PRIMER_RIGHT_1=754,22
PRIMER_LEFT_1_TM=59.985
PRIMER_RIGHT_1_TM=60.331
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.3785
PRIMER_LEFT_2_PENALTY=0.065960
PRIMER_RIGHT_2_PENALTY=0.312496
PRIMER_LEFT_2_SEQUENCE=GAGATCGCTGATGTGGATGATA
PRIMER_RIGHT_2_SEQUENCE=TGAGGTAACTGAGTCGTCATCG
PRIMER_LEFT_2=547,22
PRIMER_RIGHT_2=756,22
PRIMER_LEFT_2_TM=60.066
PRIMER_RIGHT_2_TM=60.312
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.3967
PRIMER_LEFT_3_PENALTY=0.065960
PRIMER_RIGHT_3_PENALTY=0.330697
PRIMER_LEFT_3_SEQUENCE=GAGATCGCTGATGTGGATGATA
PRIMER_RIGHT_3_SEQUENCE=AGGTAACTGAGTCGTCATCGCT
PRIMER_LEFT_3=547,22
PRIMER_RIGHT_3=754,22
PRIMER_LEFT_3_TM=60.066
PRIMER_RIGHT_3_TM=60.331
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.5530
PRIMER_LEFT_4_PENALTY=0.240472
PRIMER_RIGHT_4_PENALTY=0.312496
PRIMER_LEFT_4_SEQUENCE=TGACATGAATGTTCCCATCACT
PRIMER_RIGHT_4_SEQUENCE=TGAGGTAACTGAGTCGTCATCG
PRIMER_LEFT_4=519,22
PRIMER_RIGHT_4=756,22
PRIMER_LEFT_4_TM=60.240
PRIMER_RIGHT_4_TM=60.312
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_03850 Ps_contig_mira-and-tgicl-ass_franssen_31247
SEQUENCE=TATGTTTTTAATCCTGCATTGCAGGTCAATTTTATGTTTTTAATCCTGCATTGCAGGTCAATTTTGATAATAACAAATTTGTAACAAACTCTAACTTCCATATATTGCATTGTGGCAACTACATGAAGGTAGCTACTAGTTAGCTATGGAGCATTTGAAGAAATCTCCATCTTCTTCATCATCGTCATCAATGAGACCATCATTTCCAAGAGATCCTCGCGGCTCCTTAGAAGTCTTCAATCCAACCAGCAACTCCAGCTCGCCGGTACGCTCACCGTCAAACCTGAAAAATTGGACGGAAATCGAAGAGCCGCGCAACGAATTATCCGAGCAACATAACGAATTCTCCGATGAAGTCACCAACACTTCCTGGATGGCAATCAAGGAAGGAGAAACCGGTGCCGCAGTACAGAGAGCCGCCGAGTGGGGATTGATGCTCACGACAGATGCTGAAACTGGAAAACCACAAGGAGTGGCAGTTCGAAACTCCGGCGGAGACGAGCCGAGTGTCAAACTTGAAACGAAACGAAACTCCAATAACACCGTCAGAACTTCCGGTGAGTCTTCCGACGGTGATGATCCCCGCGGGTTTCCGAGAGTTTCGGAGGATTTAAAAGATGCTTTATCGGCTTTTCAACAAACTTTCGTGGTCTCGGATGCTACCAAACCCGATTACCCGATTTTGTATGCAAGTGCGGGTTTCTTCAAAATGACGGGTTATACATCAAAAGAAGTCATAGGAAGAAACTGTCGGTTTTTACAGGGTGCGGATACGGATCCGGATGACGTGGCAAGGATAAGGGAGGCGTTGGAAGGTGGGAAAAGTTTTTGTGGAAGGTTACTTAATTACAAGAAGGATGGAACTCCTTTCTGGAACCTTCTCACTATTTCACCCATCAAGGATGATGATGGCAATGTGCTCAAGTTAATTGGAATGCTGGTGGAGGTTAACAAACATACCGAGGGGTCCAAAGAGAAAAAGCTGCGTCCAAATGGATTGCCAGAATCCTTAATTCGATATGACGCTCGTCAGAAAGAGAAGGCTACATCTTCTGTATCTGAGTTATTAGAAGCCATGAAGCGCCCCCGGGCATTGAGTGAATCCGGACAACGTCCCTTTATCAGAAAATCAGGAGGAGGTGGAGGTTCCGAAGAAGATGAAGAAGCAGTAGAAAACAAATCAAGGAGAAAATCTGATAGTGTAGCTTCTTTTAGACCAAAACCACAAGGAAAAATTAGACATTCTATGGAACGAATCAGTGAGCTGCCTAGAAAATAAACAGAAAAACTCTCGTCGTGGTTCTTTCATGGGGTTCATGAGGAAAAGTGATTCCATTGATGAAAGTATAGATAATGAAGTTATTGTAGATGTGAGCTCTGGGAGTGAGGATGATGAGAGGGACGATTAGTTTTGAATTTGATGACAAAGAAAAACTGAGGGAAAAGAGAAAAGGTCTTGATCTTGCAACTACACTAGAGCGTATTGAGAAAAACTTTTGTTCATTACTGATCCAAGGCTTTCCTTGTACTAATCCTATAATCTTCGCTTCTGATAGTTTCTTAGAGCTTACAGAATATAGTCGTGAAGAAATCTTGGGAAAAAATTGCAGGTTTCTGCAAGGACCAGAAACTGATCCAGCAACTGTGAGAAAAATCAGAGAGGCAATTGACAACCAAACAGAAGTAACCGTGCAACTAATCAATTATACAAAAAGTGGGAAAAAGTTCTGGAACTTGTTTCATTTACAACCTATGCGTGATCATAAGGGAGAGGTACAATACTTTATCGGTGTTCAACTCGATGGTAGTCAACATGTAGAGCCTCTTCACAATTGCATTGCAGAAGATACTGCAAAGGAGGGAGAACTATTGGTAAAAGAAACGGCAGAAAATGTTGGTGAGGCAGTGAAAGAGCTTCCTGATGCTAATCAGAAACCGGATGATTTATGGATGAATCATTCAAAAGTGGTTCGCCCCAAACCTCATAGGAAGGATGATGATGCTTGGAGAGCAATCCAAAAGGTTTTAGAAAATGGAGAACAGGTAGGACCTAAAACACTTTAGGCCAATTTAAACCTTTTGGGGTCTGGAGACACCGGAAGTGTGCATCTGGTGGAGCTAGAAGGAACTGGTCAATACTTTGCAATGAAAGCTATGGATAAGGGTGTTATGCTCAATCGCAATAAGGTGCATAGAGCTTGCACTGAAAGAGAAATCCTTGACATGTTGGATCACCCTTTTCTTCCTGCATTATATGCTTCCTTTCAGACCAAAACACATGTTTGTTTGATAACTGATTATTATCCTGGGGGAGAACTATTCCTGCTTCTTGACCAGCAGCCAACAAAGGTTCTCAAGGAAGATTCTGTGAGATTTTATGCAGCTGAGGTAGTGATTGCATTGGAGTATCTTCATTGTCTAGGCATAATTTACCGGGATTTGAAGCCAGAAAATGTGCTGATCCAGAGCAACGGACATGTGTCTCTAACAGATTTTGATCTATCATGTTTAACATCTTGCAAGCCACAGCTTATACTTCCAGCTATAGAGGAAAAGAAGAAGAGGAAGAAGAAAAAGAACAAGGGACAACAAAAAAATCAACAGGTTCCAATGTTCATGGCTGAACCAATGAGAGCATCCAATTCTTTTGTTGGCACGGAAGAGTACATAGCTCCGGAAATTATAACTGGTTCAGGTCATACTAGTGCTGTAGATTGGTGGGCTCTAGGTATTCTTCTATATGAAATGCTTTATGGATATACACCATTCAGAGGAAAGACAAGACAAAAGACATTTGCAAACATTCTTCACAAAGATCTTAAGTTTCCCAAAAGCAAACCGGTAAGTCCCCATGGAAAGCAATTAATTTACTGGCTATTGCACAGAGATCCCAAGAACAGATTGGGTTCCCTTGAAGGAGCAAATGAAATTAAGAACCATCCATTCTTCAAGAATATCAATTGGGCTCTAGTTCGTTGCACGAAACCTCCTGAGCTTGATGGTCCTATCTTACTGGACAATGATGAGAAGAAAGAAGCCAAAGAAATTGACCCTGGCTTAGATGATTTACAGAAAAATATTTTCTGAAAGGTTTAAAGTATGATAGAGTGGCATTCAAGAAACACATGATGAGTGTTTGAGGTTTTGAAAAATTAGTGGAATTTTAATTTCTTGCTTATGTGTGGTGTAAAGTTTGATAATGTAATTCTTTGTAATTTGTGAAATAGAAATAGAATTTCTTTTTTAGTTTATGATGATTAATTTATCTGGCAATCCAATAGAGATGGACTATCTTGCACTTAATCTAGTCTAGAAAATCTTGTATATTTTTGTTGTAATAAGAAGTAAATTTAAATATT
TARGET=518,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1141
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.101938
PRIMER_LEFT_SEQUENCE=GAAACTGGAAAACCACAAGGAG
PRIMER_RIGHT_SEQUENCE=GTTGAAAAGCCGATAAAGCATC
PRIMER_LEFT=451,22
PRIMER_RIGHT=637,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.1225
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.110393
PRIMER_LEFT_1_SEQUENCE=GAAACTGGAAAACCACAAGGAG
PRIMER_RIGHT_1_SEQUENCE=CACTTGCATACAAAATCGGGTA
PRIMER_LEFT_1=451,22
PRIMER_RIGHT_1=694,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=59.890
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.2014
PRIMER_LEFT_2_PENALTY=0.099438
PRIMER_RIGHT_2_PENALTY=0.101938
PRIMER_LEFT_2_SEQUENCE=AAGTCACCAACACTTCCTGGAT
PRIMER_RIGHT_2_SEQUENCE=GTTGAAAAGCCGATAAAGCATC
PRIMER_LEFT_2=353,22
PRIMER_RIGHT_2=637,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.2277
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=0.215550
PRIMER_LEFT_3_SEQUENCE=GAAACTGGAAAACCACAAGGAG
PRIMER_RIGHT_3_SEQUENCE=AAGACTCACCGGAAGTTCTGAC
PRIMER_LEFT_3=451,22
PRIMER_RIGHT_3=565,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=59.784
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=115
PRIMER_PAIR_PENALTY_4=0.3150
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.215550
PRIMER_LEFT_4_SEQUENCE=AAGTCACCAACACTTCCTGGAT
PRIMER_RIGHT_4_SEQUENCE=AAGACTCACCGGAAGTTCTGAC
PRIMER_LEFT_4=353,22
PRIMER_RIGHT_4=565,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=59.784
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_03851 Ps_contig_mira-and-tgicl-ass_franssen_31254
SEQUENCE=AAAGTGTGTAGAAGAAAGCTGCAAATGTAAGGAAGTTAAAATGCACCTAAGCATATGAATGCTATTACACACCCGACAGTTTCTTTCCTTATTTCCTATTATGTACAAATTTGTCTATTGCATGATTTTTTGGGAATAAGGGAATAATCAACTGATACATCCTCTTGAACAACTAAGGCTTTCTTTTTTGCATCTTTTTCTAATGTTTCCAAATGGCTCCAATCATACTCGGTTGACTCTGCATCAATTGGTTCTTGTAGAGAAAGGGGCAATGGAACCTCAATTGGTGGGATATATCTTACTATCAACACAGTTATTGCTGATATGGTGAATGCAAGAAGAGTACCTACACTAACCATCCCAGCAAGTTGTGAGACATCCATGAAAAACGCCAAGGCTGCAGAAACAAGGCCAGTAACTACGGTACTCTTTACAGGAACATAGCTGTGCTTATTTATGTCGGAAAAAAATGGCGGAAGCAGTCCATCCCTTCCCATAGCCATCAAAATTCTTGGCTGAGGCAACATTCCACCAAGTCAATGATGAACACAGTGCTGTAATAGCCCCAGCATTTATAATGTATGCTGCCCATTGCAATCCATTGTCAGCAAATGCAGAGGATATAGGGGTGTTAGGATTGATATCATAGTAAGGTACTAAACCAACAACAACAATGG
TARGET=663,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03852 Ps_contig_mira-and-tgicl-ass_franssen_31263
SEQUENCE=AAGACAGTGGTAGTCAACGACAGAGTACGCGGGGAGTCCATCTGAGTCTATATATAGAGTGGTGACTGTATGATATANAGGNATAGCTAGTTATTTAAGGCTATACTATTGGTTAAGTTGAGAGGTATATATAAGAAGCACTCGCATAATACGCAATACATGATCAAAGAGAAATGGAGATAGAAAAGGGAATTAAGGATGGTATGGAAATGGTGGAGAAAGAAGACAGCAAGAAGATGAACCACCGTCGACGTGGAGGAATCCGAACGCTACCTTTCATTTTTGCAAATGAAGCTTGTGATAGGTTCGCAAGTGCTGGATTTCATAGTAACTTAATAAGTTACTTAACGCAAGAACTGAACATGCCATTGGTAGAAGCTTCAAACACACTCACAAATTTTGGTGGAACATCTAGCTTCACTCCTCTCCTTGGTGCTCTCCTAGCAGATTCCTTCATTGGACGCTTTTGGACCATTACCATTGGATGCCTCATTTACGAACTCGGATTGATTAGCATCACTATATCGACTACACTACCCTATTTTCGTCCTCCACCATGTCCTACACAACTCAACTGCCAAGAACCTACATCTTTACAGTTATCACCACTTTATATCTCTCTCCTTCTCACATCTCTTGGATCAGGTGGCATTAGGCCTTGTGTTGTTCCCTTTTCGGCGGATCAAGTCGACATGACGAAAGATGGTGTTGCCTCTAGGAAGTGGAACCTCTTCAACTGGTACTTTATCATCATGGGTTTTGCTTCTCTGAGTGCTTTGACCATTGTGGTTTATATTCAAGATAACATGGGTTGGAGTTTGGGACTTGGCATTCCCACCATTGCTATGCTGATTTCGATTATTTTGTTTGTGTTGGGCTTGCCGTTTTATAAGAATGTGAAACCGGAAGGTAGTCCTTTAGTCCGTTTGGCGCAAGTGATCGCCGCTGCTGTCAAGAAAAGGAACGAGATATTGCCCGATGATCCTAAGCTTTTATATCGAAATAGGGAACTTGATGCTGCTATTTCTTTAGAAGGAGATCTTTTACACTCAAATCAATATAAATGGTTGGACAAGGCTGCAATTATCACAGATGTTGATGCCAAAGATTCAAATGCACCACCAAACCTTTGGAATTTAGCAACTGTTCATAGAGTTGAGGAGTTAAAATCCATTATTAGAATGCTTCCAATATGGGCTTCAGGGATTCTACTCATAACTTCCTCATCACATTTAGGTAGTTTCGTCATTCAACAAGCTCGTTCAATGGATCGCCACCTCGGTCCGTCATTCCAAATTGCACCAGCCAATATGTCAATTTTCAGTGTCTTAACAATGATGACAGGAGTTATTCTATACGAACGCCTTTTCGTCCCGTTTGCACGTAGGTTCACAAAGAATCCGGCTGGAATAACTTGTTTACAAAGAATGGGAGTAGGGTTTGTGATTAATATTATAGCCACTATAGTTTCAGCACTAGTGGAAATAAAAAGAAAAAATGTTGCTGCAGAATACAACTTATTGGATAATCCAAAAGCGATTATTCCGATAAGTGTGTTTTGGTTAGTACCTCAGTATTGTCTACATGGAGTGGCTGAAGTTTTCATGTCTGTTGGACATTTGGAGTTTCTGTTTGATCAATCACCGGAAAGTATGAGAAGTAGTGCTACTGCTTTGTACTGCATAACTACAGCCGTTGGTAATTATATGGGCACATTGTTGGTATCATTGGTTCATAAGTATACTGGTAAAGAGAGGAATTGGCTTCCTGATAGAAACCTTAATAGAGGAAGATTGGATTATTATTATTTTCTTATTAGTGGGATTCAAGTTATAAATCTCATTTATTACATAATATGTGCTTGGTTTTACACTTATAAGCCCTTGGGTGAAGTTTGTGAAAGAGACAAGGAAGAGTATTTGGAGCAAGAGAGTACAAATATATCAGTTGATAACTTAAAAGATGGTAAGGATGAGGATAAAAGAGGGTTTACAAAAGATGAATGAAATTATGGTGTGGTTGATATTATAATGTTCAACTTCTATGTAAGTTCTATATTTAAGAAATGACAATGATGTGTATTTTTATTTATCCTCTATCCTCTACCTAAATTGTTTGCATTGGCAACTAGAGATGTATAGAAAAGTTATTTTCTATCTAAATATCTTCTTTTTTTAATAAAGGATAAGTTTTCTTACCG
TARGET=1798,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1160
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.107763
PRIMER_LEFT_SEQUENCE=TGTTGGACATTTGGAGTTTCTG
PRIMER_RIGHT_SEQUENCE=TCACCCAAGGGCTTATAAGTGT
PRIMER_LEFT=1609,22
PRIMER_RIGHT=1890,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.2570
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.248781
PRIMER_LEFT_1_SEQUENCE=TGTTGGACATTTGGAGTTTCTG
PRIMER_RIGHT_1_SEQUENCE=TCTCTTTCACAAACTTCACCCA
PRIMER_LEFT_1=1609,22
PRIMER_RIGHT_1=1905,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=59.751
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.2848
PRIMER_LEFT_2_PENALTY=0.177008
PRIMER_RIGHT_2_PENALTY=0.107763
PRIMER_LEFT_2_SEQUENCE=TGATCAATCACCGGAAAGTATG
PRIMER_RIGHT_2_SEQUENCE=TCACCCAAGGGCTTATAAGTGT
PRIMER_LEFT_2=1633,22
PRIMER_RIGHT_2=1890,22
PRIMER_LEFT_2_TM=59.823
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.3362
PRIMER_LEFT_3_PENALTY=0.228464
PRIMER_RIGHT_3_PENALTY=0.107763
PRIMER_LEFT_3_SEQUENCE=GTTTGATCAATCACCGGAAAGT
PRIMER_RIGHT_3_SEQUENCE=TCACCCAAGGGCTTATAAGTGT
PRIMER_LEFT_3=1630,22
PRIMER_RIGHT_3=1890,22
PRIMER_LEFT_3_TM=60.228
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.4201
PRIMER_LEFT_4_PENALTY=0.312329
PRIMER_RIGHT_4_PENALTY=0.107763
PRIMER_LEFT_4_SEQUENCE=GAGGAATTGGCTTCCTGATAGA
PRIMER_RIGHT_4_SEQUENCE=TCACCCAAGGGCTTATAAGTGT
PRIMER_LEFT_4=1753,22
PRIMER_RIGHT_4=1890,22
PRIMER_LEFT_4_TM=59.688
PRIMER_RIGHT_4_TM=59.892
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_03853 Ps_contig_mira-and-tgicl-ass_franssen_31267
SEQUENCE=ACNAAGAAACATAAAAGAAACATAAACAAATAGACCAATATAGAAACCATAAAAAACACATGTTGCTTAAAAGTAACCGGAAATAATCATAGCTGGCCAATACAATATTCCAAAAGGAATCAAACCAGCTTTCTAAAACCATTGAAATTGAAAAACTTTGCAGAATTTGTGGTCAAAGCTGTTCCCTTCAAGTCTTCTTTTCTCTGAATTTTGGTGATCTATTTGTGGAGTTCTTCAAAATAAAAATGGATGGTGTGACTGTAAAGGTTGAGCCAGAGCAGTTTATGGTTCTAGAATCTTCTCCTGTTGCTGTTGATGGTGTTAGTTCCAATATGGATGATGGGTCCATGATGAATTTCTCAGCAAACAAGTCGATGGCAGCATCGAGAATGAACGAGACGTTTCCTGATACAAATCAGCTTACCATCTTCTATAACGGGAATATTTGTAGCTATAACGGAATTCCAGCGGAAAAGATGCAAGAAATAATGCTCATGGCTGCTGCTGCTGCCAAGTCTACGGAAACGAAGAATATCGTGAAACAATCTCCCGTTTCTTCACCGATTCCCTCAAGGCCATCTTCTCCAGTTGCTGCTACTGATAATGTCACTCCATCGCAGGCACTCTGCTTTCCTGCAAAGAAGAGTTCCGTCTGCAGGCTTCAAGAATTTCCAATAGCACGCAGACATTCACTTCAAAGGTTTCTTGAGAAGCGGCGAGACAGGTTGGGTAGTAAAGCACCTTATCCGTCTTCGCCAAAAATGAAGGTGGCTGACAACATAGAGAACAACGTCTGCTCTGAGAATTCGCCTGATTCGGTTTCGTTGAAGGGACCAAATGAAGAATTTCAGCCAACTATATCTGCCTCTTGAGCAGAATACTTTATGTGCCGTGTTCTGTCTGTATGCATGTAGAACTACTGCATAAACTAATGTAACTGTTATTTTTCATGTTTTGAAGACATCACTACTGAACCTGGAATTGTAAGAAGTGTGACTTGGAAATTGCATGTCTCTGGTCATGTCTAGAAAGAATGCTTTCTGCTTAGATCACACATGTTAAACTGTTTTTCTTGTATCTGACTGTTTGTAGAGAACTAATTATCACTGTGTTGATGTTTGAAAANATACTTTTATGAC
TARGET=498,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2282
PRIMER_LEFT_PENALTY=0.024206
PRIMER_RIGHT_PENALTY=0.203971
PRIMER_LEFT_SEQUENCE=GATGGGTCCATGATGAATTTCT
PRIMER_RIGHT_SEQUENCE=CGGGAGATTGTTTCACGATATT
PRIMER_LEFT=338,22
PRIMER_RIGHT=551,22
PRIMER_LEFT_TM=60.024
PRIMER_RIGHT_TM=60.204
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=214
PRIMER_PAIR_PENALTY_1=0.2413
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.203971
PRIMER_LEFT_1_SEQUENCE=TGCTGTTGATGGTGTTAGTTCC
PRIMER_RIGHT_1_SEQUENCE=CGGGAGATTGTTTCACGATATT
PRIMER_LEFT_1=307,22
PRIMER_RIGHT_1=551,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=60.204
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.2413
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.203971
PRIMER_LEFT_2_SEQUENCE=GCTGTTGATGGTGTTAGTTCCA
PRIMER_RIGHT_2_SEQUENCE=CGGGAGATTGTTTCACGATATT
PRIMER_LEFT_2=308,22
PRIMER_RIGHT_2=551,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=60.204
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.2544
PRIMER_LEFT_3_PENALTY=0.050443
PRIMER_RIGHT_3_PENALTY=0.203971
PRIMER_LEFT_3_SEQUENCE=GTAAAGGTTGAGCCAGAGCAGT
PRIMER_RIGHT_3_SEQUENCE=CGGGAGATTGTTTCACGATATT
PRIMER_LEFT_3=260,22
PRIMER_RIGHT_3=551,22
PRIMER_LEFT_3_TM=59.950
PRIMER_RIGHT_3_TM=60.204
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=292
PRIMER_PAIR_PENALTY_4=0.2544
PRIMER_LEFT_4_PENALTY=0.050443
PRIMER_RIGHT_4_PENALTY=0.203971
PRIMER_LEFT_4_SEQUENCE=ACTGTAAAGGTTGAGCCAGAGC
PRIMER_RIGHT_4_SEQUENCE=CGGGAGATTGTTTCACGATATT
PRIMER_LEFT_4=257,22
PRIMER_RIGHT_4=551,22
PRIMER_LEFT_4_TM=59.950
PRIMER_RIGHT_4_TM=60.204
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_03854 Ps_contig_mira-and-tgicl-ass_franssen_31268
SEQUENCE=CTGGATCGACCAACNRAACGCGTAAGTAAGTAAAACAATTGAAGATTCCNAAAGACATTCCGGTTTTNGATCACAGCTCAGGTTGCAGTTTCGGTTGACATGGTTGAGTTGGTTTGGCATTGTTTTGGAGGTTTGTCATTTCTCATGTTCCAAATCTTGACATGATTGTAGTGGTTCAATAACATGGTTTAACGGTTATTGTTACTAGACTGATGTGCATATTCATTTGTCTTGTTTGGAGACAAAACCTGTAGGTTTGAGGTATGCAGCATGGGCATGTTCAACACCATTATGATAAGGTGAATCATTTGGCTTGATCTCAGCATTCGATACCGAAGCTTCGGGCAAACCATGAACATCCGAAGATAAATTCGTGGCATTTTCAGAAGTGCTTGAATGAACTTGCTGCGTGTTAATAGTTTCCAGATCAGTAGATTCATCCCAAGCACGGGATACACGCCTGACCACTATCTTCAGATGTTGCCTTTATCCATCTCCAGATTAACAAGAGTATATTGTCTCATTGAATTCCATCTCCTTTCCACAAACTGGACGATTGTCACTTCTCTCCATCCATTCATAGATGCAGCTAAGGTGAAAATGACTAGAGCATTTCGTCATTATCTTGGGATTCTCTTTAGTGTATTCTTCAAGACTTTGAACGGTATATCTTCAATTCATCTTTTCGAGCACATTCATTCCACTTGTCTGCGGAGTTTAGAGGCTCTGCATCATATGGTAAGGGCCTTGGAGGAGAACGGTACATGTCCGATAGAGAATTATCAAGTGACGCTGCAGATGTCATGGATGTTGTTCTGTGAAGAGATGAAGAAGTTGAAAATGCTTTGCCTCTTCGGAATATTGATGCACACGTCTATTTCTAAAAGATTTTGTACCAAACAACTGGGACACATGCCGTTCTATGAAACTGCGAAATTGAAGCGATACCGTATTTTGTTGTTGTTCTCTTTCTTTTTGTAGTCTTCACTTAATCTTCTTATTTCAGCTAACCGGTATGGAGGAAACTCTTTGATGTCCACTGCCAAGTCTTCTTTTCAAATAACTTTTTCTGTAATTGCACTTGCAGATTTCACAACATTGAGTGGTTGCTACAGTGGCTATCAAAACCTTTGGCATCGCAAAATGGAGGGCTGTCCGGTACTGACACTATTGAGAAGTTGAGAACTCACCTTACAAATTGTATCAAAACCAAAATTTGTTCCATTAAACTGGTTTGAGAATCCAACTCCACAAGACATAACAATTGCCCATCTAGTTCCAGCAGTGAGATTTGGTAAAAGTTTCCATTAAAACATTAACCATTACAAAGCCTTTTTTGATCATGATTTGGTTGATGTTGTGATTTTGTTATGGATTCGTTGCAGGGTATTGACGGCGTGATGCGTCACATGTACGTGGTGGAAGGTTGAGAATGAACGAGATATTTCCTGATACAAATCAGCTTACCATCTTCTATAACGGGAATATTTGTAGCTATAACGGAATTCCAGCAGAAAAGATGCAAGGAATAATGCTCCTGGTTGCTGCTGCCGCCAAGTCTACGGAAGCGAAGAATACCATGAAACAATCTCTTTTTCCTTCACCGATTCCTTCAAGCCCACCTTCTCCAGTTGCTGCTGCTGATAATGTCACTTCACCGCAGGCACTCTGCTTTCCTTATCCGTCTTTGCCGAAAAATGAAGGTGGCTGACAACATGGAGAACAACTTCCACTCTGAGAATTCGCCTGATTCGGTTTCGTTGAAGGGACCAAATGAAGAATTTCAGCCAACTATATCCGCCTCTTGAGCATAATACTTTATATGTTGTATTCTGTCTGTATGCATGTAGAACTACTGCATAAACTAATGTAACTGTTATTTTTCATGTTTTGAAGACATCACTACTGAACCTGAAATTGTAAGAAGTATGACTTGGAAATTGCATGTCTCTGGTCATGTCTAGGAAGAACGCTTTCTGCTTGGATCACACATGTTAAATTGTTTTTTTCTTAGTAA
TARGET=22,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03855 Ps_contig_mira-and-tgicl-ass_franssen_31269
SEQUENCE=TTCGGNGGNNCACAAGTCTCAAATATTAGTGACATGGAGTTCTCAGAAGTAGCCTTAGAGAAAGATCTAAACAACATGAGAAAGTATTATGGAAGTGGAAAAACAAAAGAAGCATCATGGAGAAAATCACAGCTAGATGGGCTGCGTAACTTTATCATTGAAAATGAAGAAAAAATCTTGAAGGCCCTTAAGAAGGATTTAGGGAAGCATCATGTTGAAGCTTTCAGAGATGAGGTGGGAATTTTAAAGAAATCCATAGATTTGGCAAGAAAATCCTTCAAACAATGGATGGCTGGCAAAGAGGCTAAACTGCCACAAATAGCACTTCTTTCAAGTGCAGAAATTGTTGCTGAACCTCTTGGTCTAGTTCTTATAATCTCATCATGGAACTTTCCCTTTGGACTGTCCTTGGAGCCATTAATCGGAGCCATAGCTGCTGGAAACATTGTAGTTCTAAAACCTTCAGAGCTGGCCCCAGCTTCTTCCTCTCTCCTATTCACTATTCTACCAAAATATTTGGACATTAATGGCATTAAGATCGTCGAAGGTGGATCAGACGTTGGAGAGATACTCTTACAGCAAAAATGGAACAAGATTTTCTTCACAGGAAGTGCACGGGTCGGGAGGTTAGTACTGTCTGCAGCGGTGGAGAATCTGACTCCAGTAACTTTGGAGCTAGGTGGGAAATGTCCAGCACTGGTTGATTCACTTTCAACCTGTTGGGACAGAGAGGTTGCGGCGAAACGAATCTTGGTGGCCAAGTATGGGTCTTGTGCAGGACAAGCATGCATAGCAATTGATTATGTTCTTGTAGAGAAGAGATTCAGTTCAACATTGGTGGAATTGTTGAAGGAAGGGATCAAGAAAATGTTTGGAGATGATCCAAAAGGTTCTAACACCATCGCAAGGATGGTTAATAAACATCATTCCTACAGAATCAAAGGTTTTCTCAATGATCCTAAGGTCAAAAAATCAGTGGTTTTTGGTGGTTCAGTGGAAGATGATAAACTATTTATTGAACCAACAATATTACTGGATCCACCACTTGATTCAGCAGTTATGACAGAAGAAATCTTTGGCCCCATCCTTCCCATAATTACTGTAGAGAAGATTGAAGATAGTATTGAGTTCATAAACTCAAAGCCTAAAGCTCTAGCTATTTATGCCTTCACAAAAAACAAAACTTTGCAGAAAAGATTGGTATCTGAAACATCATCTGGAAGTATTGTATTCAATGACGCAATTCTACAGTATGTTGCGGATACCTTACCATTTGGAGGAGTTGGTGAATGTGGATTTGGCAAGTATCATGGAAAGTTCTCATTTGATTTGTTCAGTCACTATAAAGCTGTGGCTAGGAGAAGTTACTACACTGATTTTTGGTTCAGGTTTCCTCCATGGAATCTAAACAAGCTTCAACTACTTGAAGAAGCTTACAATTTAAACTACATTGGAGTACTTCTTGCTGTACTCGGCTTGAAGAGTTCAAAGCGTTCGTTATATCAGGCTTGTAATTGATTTCCAATCATTTCCTGATTACTTATTTTTATGACAATCGTATAATAAGGTTCTGGTTATTATGTATGTCTATGTTTGTGGTATGACAATCATATAATAAGGTTCTGGTTACTATGTATGTCTATGTTTGTGACTATTTATGCTTTCAAAGTGTGTATGTTCTTCATTGATCATGTGTGTCCTTTTTGTTTTGTTATAATAAGGCTTGGTTAAGGCAAGGTTTAGATTAAGGATTCTTTTAAATGTACTTCTATCACCTTATTTATTTATATTTTAAAAG
TARGET=1777,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03856 Ps_contig_mira-and-tgicl-ass_franssen_31271
SEQUENCE=CTATAAAAGCTCATACCATGGCAATTRCACAAAAGCCATAACAGTAGTTATTCAGAGCATGACAGTAATACAAAAGCCACAAAAATGATTAATTTAACAAAGTTTACAGCAGCAACTAGCACTATAATTGTCCATTCAATGAGATATTCTATTTTGTATCCAAATGTCAAGATTATTGCAATGTAGTACTCATACACATACATAATAAGAACATAATGTATTATAAATGTTTTTGGTCCATTAATCCAAAGCCAATGTGTAACTCATTCCTTCCATCTCTGCTCATGAGGAAATGAAACCTCAAGAGCACATTGCCTTCTCCACCACTTCCTGTAATTCTCCATTCTTATATGCCTCAACAGTGATATCACATCCACCGAAAAACTCTCCATCAATGTATAGCTGAGGAAAAGTAGGCCAACTGCTATACTCCTTAAGTCCCTGGCGCACCATATCATTATCCAATATGTTTACAGTTTCAAAAGAAGCATTCATAGACTTCAAAATTTGCACCACTGTATTTGAGAATCCACACTGTGGAAAATCCCTAGTACCCTTCATAAACAAAACTATTTTGTTTGAAGTAACAACTTTATCCAACGTGGTCTTCAATTCAGGAGTCAACGCAGAGCAGCGAGTTGTGGTGGAAGAGCGTTTGATATGTAGTTTGGGTTGGGAATGGAAAACGGTGTTTTGAGAGAGAAGAGGAGATGAACGAGGTAGTAGAGACAGGGACGAGGTTGTTGTTGTTGTTCCAGAAACGTGGGAGTGGAAGCATGATGTTGTTGGGTTGAATTGTAGCTGTGAAGAAACTGGGTTAACGCACCATGTGAAAGACATGTTCTGAGTTCTAACTTCCACTCACAATTCTCTTCCAACAAATATCACTTGTTTGATTTCTCCGATTTTTTTTTCTCAACTGATTTTTCCGATTGTTATTCNCCCCGAC
TARGET=740,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03857 Ps_contig_mira-and-tgicl-ass_franssen_31272
SEQUENCE=ATTAATGGAACTTACCATCTACTCCAAGTTTGCAATTAAGGTGACAGTTACATTCTACAAACCTGAAACATGTCTTCTTCCACTACTTGCTGATCCTGTCTTTTTCTTTTCTTCACCGTTTCAGATTTAACCCTTCTCTCACCAAACCCACCTTCCATCTTCTCCACTACACAACCTTTGTCCATAAATTTCGAAATTTGTCTCTCTGGGTTCTAGAGAGAGAAAAAATGTCTGGTAGAGATCTTGAGAGAGAAGGGGCAAACAAGAACTATTCTTCTTCTATTCCTCCTCCTCCTTCACGTGTTTATGATCCAGAAACTAGTTGGACATCATGGCTTGTTCCTTTGTTTGTTGTGGCGAATCTTGTTATTTTCATTCTAGCCATGTATATTAATGATTGTCCAGGTAAAGTTCATGCGGTTGAAGGTGATTGTGTGTTCAAATTTCTTGGGAGATTCTCTTTTCAGCCGACCAAAGAGAATCCTTTGTTTGGTCCTTCTTCTTTAACACTAACGAAGATGGGAGCTCTTAAGTGGGATGCTGTTGTGTATCATCACCAAGGATGGAGACTTGTCACATGCATATGGTTACATGCTGGAATGATTCATTTGGCAGCAAACATGTTAAGTCTGGTTTTCATTGGCATTCGCCTTGAACAACAATTTGGTTTCGTGAGGATAGGAATCATTTACCTTGTATCTGGATTCGGTGGGAGTATCCTATCTTCTCTATTCATTAGAAACAACATCTCGGTTGGTGCTTCTGGCGCTCTTTTTGGACTTCTTGGAGCAATGCTTTCAGAACTTATCACAAACTGGAGTATTTACACCAATAAAGTAGCTGCTTTGGTCACACTTTTGTTTATTGTTGTGATCAATCTTGCGATTGGAATGTTGCCACATGTTGACAATTTTGCTCATATTGGGGGATTCTTGACAGGATTTCTCCTTGGTTTCATTTTCCTTCCCCGTCCTCAATTTGGGTGGTTAGCACAGCGACATGTTCCTGCTGGTGCTCGACTCAAATCCAAATACAAGGTTTACCAATATGTCCTATGGATTCTCTCTGTTATTCTAGTGATTGCCGGGTTATGCACTGGCTTACTGATGCTGTTACGCGGCGAAAACGGATACGATCACTGCCATTGGTGTCACTACCTAACATGTGTTCCAACTTCAAAATGGAAATGCAGTGACAGTTAAACTATTCTTGTTCTTTTATGTATAGCTATGACTTTTACAGATTGTTTCTTTTTTTGGTTAATTCGTTATAAGAATCAGCTATTAGTTTGTGTTTGGATTTATGTGGGTGTATGTATACACAGATATATAAAGGAATTTATTTGTACATGAAGTTTCTATTTCAATAATATATTCTACCTGAGTTTCTTGTT
TARGET=284,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3307
PRIMER_LEFT_PENALTY=0.237699
PRIMER_RIGHT_PENALTY=0.093026
PRIMER_LEFT_SEQUENCE=GAGAAGGGGCAAACAAGAACTA
PRIMER_RIGHT_SEQUENCE=AGAGCTCCCATCTTCGTTAGTG
PRIMER_LEFT=249,22
PRIMER_RIGHT=528,22
PRIMER_LEFT_TM=59.762
PRIMER_RIGHT_TM=59.907
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.4162
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.306698
PRIMER_LEFT_1_SEQUENCE=CGAAATTTGTCTCTCTGGGTTC
PRIMER_RIGHT_1_SEQUENCE=CACAACAAACAAAGGAACAAGC
PRIMER_LEFT_1=191,22
PRIMER_RIGHT_1=355,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=59.693
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=0.4162
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.306698
PRIMER_LEFT_2_SEQUENCE=TTCGAAATTTGTCTCTCTGGGT
PRIMER_RIGHT_2_SEQUENCE=CACAACAAACAAAGGAACAAGC
PRIMER_LEFT_2=189,22
PRIMER_RIGHT_2=355,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.693
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.4162
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.306698
PRIMER_LEFT_3_SEQUENCE=TCGAAATTTGTCTCTCTGGGTT
PRIMER_RIGHT_3_SEQUENCE=CACAACAAACAAAGGAACAAGC
PRIMER_LEFT_3=190,22
PRIMER_RIGHT_3=355,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.693
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.5235
PRIMER_LEFT_4_PENALTY=0.216800
PRIMER_RIGHT_4_PENALTY=0.306698
PRIMER_LEFT_4_SEQUENCE=TTCACCGTTTCAGATTTAACCC
PRIMER_RIGHT_4_SEQUENCE=CACAACAAACAAAGGAACAAGC
PRIMER_LEFT_4=111,22
PRIMER_RIGHT_4=355,22
PRIMER_LEFT_4_TM=60.217
PRIMER_RIGHT_4_TM=59.693
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_03858 Ps_contig_mira-and-tgicl-ass_franssen_31275
SEQUENCE=GTCGCCCTATCCATTCTCTGCAAAATCAATGGCTGCCAAGATCATGTTGCTATCTCCACCTCCAATCCCTCTCCGTTCTCTCCCATTTCACACAAACAAACTACACCCATGGAACCATCAGCACCATCTTCCAACTCCAAAACCAGAGAAACTTCGATTATCCCTTCATGAGAGTGCCGAATTCATCACCCAACAACAACAACTACTAAACCCCAATTCTCTTTTCTTGCTTACAGAGACTGCTGGTTATTCTTTGGCTAGTTATTATACTTCTCTTGGTCTCTTTGTTATATCTGTTCCTGGTCTTTGGTCCCTCATCAAACGTTCTGTTAAATCCAAAATTGTGAAGAAGACATTTGTAGATGAAGTTGGAAATAAGGCACCAAATCAGGTTGCAGGGGAGGTGCTATCATTCTTTACTCGTAATAATTTTTCAGTGGTTGATAGAGGAGAAACAATCACGTTTGAAGGAGTGATGGTTCCTAGCAGGGGGCAAGCAGCACTGTTAACTTTCTGCACTTGCATCAGTCTTGCAAGTGTTTCCCTTGTGCTCACCATAACTGTGCCAGATGTTGGCAATAATTGGTTTGGGTTAACCATCTTAAGTCCATTGGCGGGTGCTTATTACTGGACTAGAGCATCCAGAAAGGAGCAAATTAAGGTGAAACTGACAGTTAAAGAAGACGGAACCTTATCAGAGATTGTTGTTCAAGGTGACGACCAACAAGTCGAGCAAATGAGAAAGGAACTAAAGTTTAGTGAGAAGGGCATGGTTTATGTTAAAGGCCTTTTTGAAACATGACTTGTTTTAATCATATGGCTGGACAGAATGTACACAAATGAATAGGCATGTGAAAAACAGCCAACAGAAATGTTAGTGAACAATTATGTTAAATCAGCACGCTGACAGTTTTGACATTTGCCATAGGCATCTAGTACATTTTAAGAATTAAGATCATGATGTTTCTTGAATTTAAAGATGTG
TARGET=191,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1890
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.180765
PRIMER_LEFT_SEQUENCE=CTCTCCCATTTCACACAAACAA
PRIMER_RIGHT_SEQUENCE=CCAGCAGTCTCTGTAAGCAAGA
PRIMER_LEFT=77,22
PRIMER_RIGHT=245,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.819
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=0.1929
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.180765
PRIMER_LEFT_1_SEQUENCE=CCAACTCCAAAACCAGAGAAAC
PRIMER_RIGHT_1_SEQUENCE=CCAGCAGTCTCTGTAAGCAAGA
PRIMER_LEFT_1=130,22
PRIMER_RIGHT_1=245,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=59.819
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=116
PRIMER_PAIR_PENALTY_2=0.1996
PRIMER_LEFT_2_PENALTY=0.018835
PRIMER_RIGHT_2_PENALTY=0.180765
PRIMER_LEFT_2_SEQUENCE=AACAAACTACACCCATGGAACC
PRIMER_RIGHT_2_SEQUENCE=CCAGCAGTCTCTGTAAGCAAGA
PRIMER_LEFT_2=94,22
PRIMER_RIGHT_2=245,22
PRIMER_LEFT_2_TM=60.019
PRIMER_RIGHT_2_TM=59.819
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.2043
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.180765
PRIMER_LEFT_3_SEQUENCE=ATCTTCCAACTCCAAAACCAGA
PRIMER_RIGHT_3_SEQUENCE=CCAGCAGTCTCTGTAAGCAAGA
PRIMER_LEFT_3=125,22
PRIMER_RIGHT_3=245,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=59.819
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=121
PRIMER_PAIR_PENALTY_4=0.2125
PRIMER_LEFT_4_PENALTY=0.031769
PRIMER_RIGHT_4_PENALTY=0.180765
PRIMER_LEFT_4_SEQUENCE=GTTGCTATCTCCACCTCCAATC
PRIMER_RIGHT_4_SEQUENCE=CCAGCAGTCTCTGTAAGCAAGA
PRIMER_LEFT_4=45,22
PRIMER_RIGHT_4=245,22
PRIMER_LEFT_4_TM=59.968
PRIMER_RIGHT_4_TM=59.819
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=201
=
PRIMER_SEQUENCE_ID=SSR_03859 Ps_contig_mira-and-tgicl-ass_franssen_31290
SEQUENCE=GTCTTNATTTAAAACCCAGCATAGAACGAATTGATAATGGAGTTTAACATAACAGCCAACAATTGAAGCATTCACTAAAACTTATCTACTATTCAGTCAAATTTGTCACAAGAAGGCCTTTAAGATTAAAGCTATACATGAAATTTAAGTGCTAATAAAAAAAGCTCCATGTCAAGTAGTCTAAAGAGTATACACTCAGTTTTGCAGAGATAGATGCACAAAAGGATTGGATTCAGAGTTGTTTTCCTCCAAATCAGCTGCAACATCCACCTCCTGCAGCAACAGATCCATCTCTGCCTCCTGTTTGGCCCTGTGGACGTCCATAGCCGATTTTTATACCACATGTCTCATTGGACACATCAAAAACACCATCCTGGATGTTCTGAAGAATCTTTGCAGCAGTCTTTATGAAAGCCTCTTCTACATTTTGCGCTGTCTTGGCGGAAGCTTCCATGAATAAAAGTCCATTTTCCTTTGCAAATTGCTCTCCCTCCTCTTTGCTGACTGCCCTTCGGTGAGACAGATCACCCTTGTTCCCTATGAGCGTGATTGTCATGTTAGGATTGGCATGCTGCCGCGCATCTTCTAGCCAACTTGCTAAATGATTAAAGGTTTCTCTCCTTGTAATGTCATACACTAGAAGTGCCCCTGCTGCTCCTCTGTAGTAAGATCTAGTGATAGATCTAAATGATTCTTGCCCAGCAGTATCCCATATCTGAAGCTTAATCGGCCGTGAATCAATGGTGAGCATACGCGCACCAAACTCAACACCAATGGTGAGATCATGAACAGGTTGAAACCTCTTGTCGGTGAACTGAAGGAGCAGGCAAGATTTCCCTACACCTGTGTCACCGATGATGATGTACTTGAAGAGATAGTCGTACGACATGGTTTTATAGATAAAACGACGTCGTTTGAAGGGATTTAGAAGAAGGTGAAGATGAAGAAGTTAGCGTTTTATGTTAAGGTTTGAGTGTNGCTGAATGATGATGATGATGGATTGATTTTGAATCCCAATCGAAAAAACAATGTGACGTATGATCGGAGCATGAGAAAGAAACATCTG
TARGET=984,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2484
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.247631
PRIMER_LEFT_SEQUENCE=GACGTCGTTTGAAGGGATTTAG
PRIMER_RIGHT_SEQUENCE=CCGATCATACGTCACATTGTTT
PRIMER_LEFT=906,22
PRIMER_RIGHT=1044,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.752
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=139
PRIMER_PAIR_PENALTY_1=0.2484
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.247631
PRIMER_LEFT_1_SEQUENCE=ACGTCGTTTGAAGGGATTTAGA
PRIMER_RIGHT_1_SEQUENCE=CCGATCATACGTCACATTGTTT
PRIMER_LEFT_1=907,22
PRIMER_RIGHT_1=1044,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.752
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=0.2576
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.256829
PRIMER_LEFT_2_SEQUENCE=GACGTCGTTTGAAGGGATTTAG
PRIMER_RIGHT_2_SEQUENCE=TCCGATCATACGTCACATTGTT
PRIMER_LEFT_2=906,22
PRIMER_RIGHT_2=1045,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.257
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=0.2576
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.256829
PRIMER_LEFT_3_SEQUENCE=ACGTCGTTTGAAGGGATTTAGA
PRIMER_RIGHT_3_SEQUENCE=TCCGATCATACGTCACATTGTT
PRIMER_LEFT_3=907,22
PRIMER_RIGHT_3=1045,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.257
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=0.4008
PRIMER_LEFT_4_PENALTY=0.153161
PRIMER_RIGHT_4_PENALTY=0.247631
PRIMER_LEFT_4_SEQUENCE=ATTTCCCTACACCTGTGTCACC
PRIMER_RIGHT_4_SEQUENCE=CCGATCATACGTCACATTGTTT
PRIMER_LEFT_4=831,22
PRIMER_RIGHT_4=1044,22
PRIMER_LEFT_4_TM=60.153
PRIMER_RIGHT_4_TM=59.752
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_03860 Ps_contig_mira-and-tgicl-ass_franssen_31299
SEQUENCE=ATCGACATTGAATCGTAATGTGGAATGCAAGTATTTGAAACTNGTCTGATATCATCATCATCGTATATGATAAAGCCACACTACGCGGTCCACGTCATCATCCTCAACCTCTTTCCCCACATTACAGAAACAACAAAAAATCCTCTATCTCTCTCTCTCTCCGATTTGAATCTCGCTGACTCGGAGTCTTCCTCTCTGGATTTCTTATTGAAGATGCGACGCGCTGCATCCAAGAAAACGGGTCAATCCAATTCCAACCCCTCCGTCAATTCCTCCGCCTCGGATCTATTCCGCTCCGCTTCGAGTAAAGCTAACTCAAAGGAACTGGAGCGAATTGATAGTCTATTTTATTCGTATGCCAATGGATCTAGTGGGGTGATTGACCCAGAAGGAATTGAAGCACTTTGTGCTGATATGGAGGTGGATCATACTGATTTGAGGATTTTGATGCTCGCGTGGAAAATGAAATCTGAGGAGCAAGGATATTTTACCTTGGATGAGTGGAGGAGAGGCTTAAAAGCACTAAGGTCTGATACAGTAAGTAAATTGAAAAAGGCACTTCCAGACCTAGAAAAGGAGGTCAGGAGGCAGTCAAATTTTTCAGATTTCTATTCATATGCTTTTCAATATTGTCTAACAGAGGAGAAACAGAAAAGCATTGATATAGAGAGCATATGTGAATTACTTACTCTTGTTTTGGGTTCAACATTTCCAGTTCAAGTCAGCTTATTTGTGGAATATTTGAAGAATCAAAATGATTACAAGGTCGTAAACATGGACCAATGGATGGGATTCTTCCGGTTTTGCAATGAGATAAGTTTTCCGTCGCTTAATGACTATGATCCCGAACTTGCATGGCCCTTAATCCTGGATAATTTTGTTGAATGGTTGCGAGAAAAGCACACGTAATCTGATATATTTCAGCCATCTGATTCTCTGTACCATGCAATCCCTGCACTCCTTTGAACAAGAGAAGCTGTTGGATTACCGAATCAGATGGGTGCTTTATGGCTCGTTTATTGTACTCAACAAACTATACTGTACTTCTGTTGCCCAGTTTCTTAATGGGGAATTCTAAATATAAACTATTGTTACTTCTTTTTTTAAAAAAACATTTCATCTTATAGATGCAGTTGAGGTTAGATACAAACTTGTACTCTGTAATCATTACCAATGTCAACATCATATATTCTATTCATATCATTCACCTAAGAGCTTCAATGCTCTTGAGCCTCTAGTAGTTTGAGAAGTTAATGTAAGGAAAATTGATACTARNNTTCTTATTTTCTTAAGAAAAAACG
TARGET=51,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03861 Ps_contig_mira-and-tgicl-ass_franssen_31299
SEQUENCE=ATCGACATTGAATCGTAATGTGGAATGCAAGTATTTGAAACTNGTCTGATATCATCATCATCGTATATGATAAAGCCACACTACGCGGTCCACGTCATCATCCTCAACCTCTTTCCCCACATTACAGAAACAACAAAAAATCCTCTATCTCTCTCTCTCTCCGATTTGAATCTCGCTGACTCGGAGTCTTCCTCTCTGGATTTCTTATTGAAGATGCGACGCGCTGCATCCAAGAAAACGGGTCAATCCAATTCCAACCCCTCCGTCAATTCCTCCGCCTCGGATCTATTCCGCTCCGCTTCGAGTAAAGCTAACTCAAAGGAACTGGAGCGAATTGATAGTCTATTTTATTCGTATGCCAATGGATCTAGTGGGGTGATTGACCCAGAAGGAATTGAAGCACTTTGTGCTGATATGGAGGTGGATCATACTGATTTGAGGATTTTGATGCTCGCGTGGAAAATGAAATCTGAGGAGCAAGGATATTTTACCTTGGATGAGTGGAGGAGAGGCTTAAAAGCACTAAGGTCTGATACAGTAAGTAAATTGAAAAAGGCACTTCCAGACCTAGAAAAGGAGGTCAGGAGGCAGTCAAATTTTTCAGATTTCTATTCATATGCTTTTCAATATTGTCTAACAGAGGAGAAACAGAAAAGCATTGATATAGAGAGCATATGTGAATTACTTACTCTTGTTTTGGGTTCAACATTTCCAGTTCAAGTCAGCTTATTTGTGGAATATTTGAAGAATCAAAATGATTACAAGGTCGTAAACATGGACCAATGGATGGGATTCTTCCGGTTTTGCAATGAGATAAGTTTTCCGTCGCTTAATGACTATGATCCCGAACTTGCATGGCCCTTAATCCTGGATAATTTTGTTGAATGGTTGCGAGAAAAGCACACGTAATCTGATATATTTCAGCCATCTGATTCTCTGTACCATGCAATCCCTGCACTCCTTTGAACAAGAGAAGCTGTTGGATTACCGAATCAGATGGGTGCTTTATGGCTCGTTTATTGTACTCAACAAACTATACTGTACTTCTGTTGCCCAGTTTCTTAATGGGGAATTCTAAATATAAACTATTGTTACTTCTTTTTTTAAAAAAACATTTCATCTTATAGATGCAGTTGAGGTTAGATACAAACTTGTACTCTGTAATCATTACCAATGTCAACATCATATATTCTATTCATATCATTCACCTAAGAGCTTCAATGCTCTTGAGCCTCTAGTAGTTTGAGAAGTTAATGTAAGGAAAATTGATACTARNNTTCTTATTTTCTTAAGAAAAAACG
TARGET=148,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03862 Ps_contig_mira-and-tgicl-ass_franssen_31302
SEQUENCE=AATTTTAGTTGACATGTTGTTTTTGTAGCTTTNGCCGATGAGTCAGAAAAGCGATTATGAACGTGTTAGCGAGTAGAACAAGTTTTGTGGCTCAACAACCGGAAAGCGCTTCTACACCGGCATAATCACCATCAACAACAACAACGATATCTCTTCGTATCATTATAATATATTCTTTTAATCCTTCCGGCGCCACTTGTTTCGCCTTTTACAAATTTAAATTATGATAATCGCGTTCGCCGGAGAAAAAAGCGATTATAAAACGTGCGGTGAACAACCTCATCATAACTAAACGGAATAACGAAAAAAAATAGGGTAAAAAACAATCGTTGCGGAGTAAAGCAGTTCATCTTGTTTCGGATCTCACCACTGTTCTTCTCAACCCTATTTCTGACCATAAC
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8215
PRIMER_LEFT_PENALTY=0.740565
PRIMER_RIGHT_PENALTY=0.080887
PRIMER_LEFT_SEQUENCE=CTTCTACACCGGCATAATCACC
PRIMER_RIGHT_SEQUENCE=AAGATGAACTGCTTTACTCCGC
PRIMER_LEFT=108,22
PRIMER_RIGHT=352,22
PRIMER_LEFT_TM=60.741
PRIMER_RIGHT_TM=59.919
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=245
PRIMER_PAIR_PENALTY_1=0.8402
PRIMER_LEFT_1_PENALTY=0.199358
PRIMER_RIGHT_1_PENALTY=0.640802
PRIMER_LEFT_1_SEQUENCE=CGATTATGAACGTGTTAGCGAG
PRIMER_RIGHT_1_SEQUENCE=GTTATGATGAGGTTGTTCACCG
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=288,22
PRIMER_LEFT_1_TM=59.801
PRIMER_RIGHT_1_TM=59.359
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.8615
PRIMER_LEFT_2_PENALTY=0.220659
PRIMER_RIGHT_2_PENALTY=0.640802
PRIMER_LEFT_2_SEQUENCE=CAGAAAAGCGATTATGAACGTG
PRIMER_RIGHT_2_SEQUENCE=GTTATGATGAGGTTGTTCACCG
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=59.779
PRIMER_RIGHT_2_TM=59.359
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=0.8781
PRIMER_LEFT_3_PENALTY=0.740565
PRIMER_RIGHT_3_PENALTY=0.137489
PRIMER_LEFT_3_SEQUENCE=CTTCTACACCGGCATAATCACC
PRIMER_RIGHT_3_SEQUENCE=ATCCGAAACAAGATGAACTGCT
PRIMER_LEFT_3=108,22
PRIMER_RIGHT_3=361,22
PRIMER_LEFT_3_TM=60.741
PRIMER_RIGHT_3_TM=60.137
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.9585
PRIMER_LEFT_4_PENALTY=0.877649
PRIMER_RIGHT_4_PENALTY=0.080887
PRIMER_LEFT_4_SEQUENCE=GCTTCTACACCGGCATAATCAC
PRIMER_RIGHT_4_SEQUENCE=AAGATGAACTGCTTTACTCCGC
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=352,22
PRIMER_LEFT_4_TM=60.878
PRIMER_RIGHT_4_TM=59.919
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_03863 Ps_contig_mira-and-tgicl-ass_franssen_31304
SEQUENCE=AACAATTCAAGTCGTAATCGTTGATTTCAGTAGCTGGACTGGTGACCCTTTTGAGTATGATGAGAAGCAAGGTTCTGATGTGGTTGTAAGATTCTGCAAAGATGTGGAGAGTAGATCGCAAACGGGATATGTTGATTTTGGCCGATTTGATAGGTTCAACTACTTTGTTGCTRGTTCTGGCCAATATGTATTTTGTTCAAGAATTTTATAATGGTGACCTAATGGGCTGTGAACAAAGTTATGACAAAATGGGACGAACAGCTCAGGTAAATGTCATATGTGGGAGCTGTCCTAATGGACAATGTAAAGGTCGTCCTGGATGCATATGTCACGTCGCACATGAATCGAACTGCAGAGTTTTAATTGAACTTGCAATTTCATGTGAGAAACCGGGCCCACAAATATTTCAAGGCTTCACTGTTGGTTTTCATCCACGATCATGGGAACTTGTTTATAATGGCATGACTCAGTTCGGTTTTGAGAAAACCCATCATGATTTTAGCTTTCAGACATCACAAACTCAAGTAGTCCTATTTACAAGTGCAATTGCTTCACTCTCATCCTTGGTTCGTAAACCTATTTTGAAGGTTCTTCCAAATGATGGTTTGAGAGTGAAATTGTCTGGATCTGCTGCTAGTGGGAAGCCTCCGACAACTTTGTCACCCACAATGTTGATCCTTGATTGGAGATGTGAGGTAACCCGTAACACGCCATATGAAGTTAACATCACAATCCCGATAGAAGGATATGAACCCATTCAATTTGTGCTTACAAAAATGTGCGACTACAAACAAGATCAAGGAGGTGGTGCCACAAGAGGATGGGCAATATTTGGAGTGATATCTTGCATATTCTTTGTCTCATCAACAATTTTTTGCTGTGGAGGGTTCATTTACAAGACTAAAGTGGAGCGCCAGCGTGGGGTTGATGCATTGCCTGGCATGACATACCTATCTGCCTGCTTAGAGGCAGTTAGTGGGGCTGGGCAAGGTTACTCACGGCCAGAAGACTCGTACGCCTCCGGCGAAACCTCTTGGGAGCGCCCTCCTGGTCCTTCACAACCTCAAGCTTCATGGAGACCAACAGAGAGAAAATATGGCGCCATTTGAACGCATCCTGGCCATTTGAACAGGTCCTGCCACCGATTTTGTTGTTGTTGCTCTGTACCATTATTTTATAAGAATGGGAATTGTTTCTATGCCAAATGTTATTTGGATTGACTCATTCATACAACAGAACCTCAGTCTGATTTATTTACAGGTAAGTAAAGAAGAGATAGTTGTAGTAGTTTGAATCTAGCAGCTGAATTGAAGTTAATGATAGGTCGAATTCAAGTRNATGACGAAAACAAAAAGGGTNTTGAACTAAAAAACTT
TARGET=1148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03864 Ps_contig_mira-and-tgicl-ass_franssen_31314
SEQUENCE=TTGTATCAGTACTATCATATGTTGCATGAAAGACCCTCAGCTGGTGCATAGGTTTTACCTAAGAAGTCAGTAAGCTTGGTCGTCCTGAACCAAATGGGATAATGAGCATCACTACCACTATGACTGAAATTGACAAGAAGATACTATCATTGGGTTATAGTGAGCTGAATGCAGAGATTATATCTGTGGATGCACAAGAGTCCTATGGTGGGGGAGTGATTGTCTTGGTGACGGGGTTTATGACGGGAAAGGATGATATTAAACAGAAATTTACTCAAAGTTTCTTCCTTGCTCCTCAAGAGAAAGGCTACTTTGTTTTGAATGATGTTTTCAGATATGTTGATGAAAATGGAATAGAAGAGTCTGATCATGTAATTGTATCACCTACTTCTACTGACAATGTGTCAGAACCACCTGTGCTAGAGGCGCAAGTTCCAGAGCAAATATCTGTGACTGTTGAGGATGGTGTTGGGGAAGAAGTCTACAATCCTGAAAATGAGCAAGTGTCTGTTGAAGAAGAGGAAGTTCCTGTTGCTGAAGTTCTTGATGAAATTCCTGATGATTCACAGATGATATCTGGATTGGCATCCCAAATTGAAGAAGTACCAAAGAAGTCATATGCCTCTATTGTTAAGGTAATGAAAGAGGGTGGAGTACCATCCTCCATAGTGACAGCTGTTTCTGTGAAATCTGTCCGTAAGATTCAAGAACAGCAGAGCACCACTGCACCTCCACCATTCAGTGGGCCAGAAACAAATGGATCTAGTATAAACACCAACGAAGGTGGAAACAATCAAGAAACTGAAGTTGAGGGCTATTCAATTTATGTGAAAGGGCTGCCTGGAAATGCTACGCCTGCACTTCTCGAGAATGAGTTCAAGAAGTTTGGCCCTATTAAAAGTGGTGGTATCCAAGTTAGGACGCAAAAGGGTTTTTCCTTTGGCTTTGTGGAGTTTGAAGTGGAAAGTGCTGTGCACAAGGCAGTCGAGGTTTCACCAGTTCAGATCAGTGGCCGTTACGTTATTGTTGAGGAAAAGAGATCAACTAATCGAGGTTATAGAGGGCGATTCTCGACTGGAAGGACACCTGGATTCAGGGGTGAGGGAGCAAGAGGACGAGGAAACTATGGAAATAGCCGAAGTTTTGGACGTGGAGACTTCAACGGTCGCGGAGACATTAACGGCCACGGAGACTACAACGGTCGTGGAGACTTCAACGGTCGTGGAGACTTCAATGGACGTGGAGACTTTAACGGAAGAGGGGAATATGGATATAGGAATGGTAACCGGGGAGGATTTTCAAGCCGTGGAGGTGATGTGTATCAGAGAAACGACCATATGGGAACCAATGGTGGCCGCATGAATCGTGCTGGTGGTTCTTCTGTTAACTCGGCACCCAAACCTACTGGTGCTCAACGTGTTCCTGCCTCTGCTTGAGGAAGCTATGCATCTAAGAGTCTGACTGATTGATAGTTTATTACAATTTGCATTAACTGAAGCATTTGGAACTAGAAAGAATTAAATAGCAAACCTTTTTTAGATGAGTTAAGTTTACAAAGTCCCCTTTTTAGGTCATTTTCCTGATATTGGTGGTCTTGGGAAAAGGTAGTTTTCAGTTTCTATTCATTATATAATGAGTCTAACGGTAGTTTTTGTGCTTCCCCAGTTTTTATGAAAATATATATATTTGGG
TARGET=1677,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03865 Ps_contig_mira-and-tgicl-ass_franssen_31323
SEQUENCE=GGGGATTACTAACACTCAGCCACACCACNAATGAAAACAATATCAAGAATGGTGTTACCAAGTGTTGGTGTACAATTCACTCCATTTTCAATCCACCGAACACACACAATACGACCAACAACAACAACAACAACAACACATTCTTTCAAAAAACATTCACTTTCAAACTCAACCATAAACATGTCATCGTTTCCCAATAACACGACCAGGAAACTTCCAATTCTTCTGTTTGATATCATGGACACCATTGTTCGCGACCCTTTCTACAAAGACATCCCTTCTTTCTTCGGTATGTCCTTCAACGAACTCATAGATTCCAAACACCCAGCCGCTTGGATTCAATTCGAAAAGGGTCTCATTGATGAGGTGGAGCTAGCAAGAATTTTTTTTAAGGATGGAAGGGATTTTGATTTAGAAGGCCTTAAAGTTTGTATGAGAAATGGATATTCATACATAGAAGGGATTGAACAATTGCTTCTAGCCTTAAAGAAAAATAACTTTGAGATGCATGCTTTTACTAACTATCCTATATGGTATCAGTTGATTGAAGACAAGTTAAAGCTATCAAAATATTTATCTTGGACATTTTGTTCGTGCACTTATGGAAAGAGGAAACCAGATGCTGAATTCTATATGGAAGTTGTGAGGCATCTTGAAGTTGATCCNNCATATTGTATTTTCATAGATGACAGACAAAAAAATGTGGAGGCAGCAACTGAAGTTGGGATCAAGGGTGTACATTTCAAGAATGTTGATTTACTGCGCGAAGAACTGTCATTGATGGGGGTTGACATTTTAAAAGATGAAGATCAATAGCTTTTTATTTATTTATTTATTTGAATACAACAATTTCTTTTACATGGTTAAAATGACTTCGGAAGGTCAATTCATGGGCCTTTTTAAAATTAGGGCCTAAACTNYAAAAACCCCTATTTTAGAGATCTAAATTTTAGGGAAATAGTAACTCTCCACCTAAGTAGTGGAAAACACCTTATAAAAATTCAAAATTGTCGATAAATTTTCGT
TARGET=116,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03866 Ps_contig_mira-and-tgicl-ass_franssen_31323
SEQUENCE=GGGGATTACTAACACTCAGCCACACCACNAATGAAAACAATATCAAGAATGGTGTTACCAAGTGTTGGTGTACAATTCACTCCATTTTCAATCCACCGAACACACACAATACGACCAACAACAACAACAACAACAACACATTCTTTCAAAAAACATTCACTTTCAAACTCAACCATAAACATGTCATCGTTTCCCAATAACACGACCAGGAAACTTCCAATTCTTCTGTTTGATATCATGGACACCATTGTTCGCGACCCTTTCTACAAAGACATCCCTTCTTTCTTCGGTATGTCCTTCAACGAACTCATAGATTCCAAACACCCAGCCGCTTGGATTCAATTCGAAAAGGGTCTCATTGATGAGGTGGAGCTAGCAAGAATTTTTTTTAAGGATGGAAGGGATTTTGATTTAGAAGGCCTTAAAGTTTGTATGAGAAATGGATATTCATACATAGAAGGGATTGAACAATTGCTTCTAGCCTTAAAGAAAAATAACTTTGAGATGCATGCTTTTACTAACTATCCTATATGGTATCAGTTGATTGAAGACAAGTTAAAGCTATCAAAATATTTATCTTGGACATTTTGTTCGTGCACTTATGGAAAGAGGAAACCAGATGCTGAATTCTATATGGAAGTTGTGAGGCATCTTGAAGTTGATCCNNCATATTGTATTTTCATAGATGACAGACAAAAAAATGTGGAGGCAGCAACTGAAGTTGGGATCAAGGGTGTACATTTCAAGAATGTTGATTTACTGCGCGAAGAACTGTCATTGATGGGGGTTGACATTTTAAAAGATGAAGATCAATAGCTTTTTATTTATTTATTTATTTGAATACAACAATTTCTTTTACATGGTTAAAATGACTTCGGAAGGTCAATTCATGGGCCTTTTTAAAATTAGGGCCTAAACTNYAAAAACCCCTATTTTAGAGATCTAAATTTTAGGGAAATAGTAACTCTCCACCTAAGTAGTGGAAAACACCTTATAAAAATTCAAAATTGTCGATAAATTTTCGT
TARGET=820,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03867 Ps_contig_mira-and-tgicl-ass_franssen_31328
SEQUENCE=CTTTAGAGAATGTTATAATCATTATTTCATCTCAATTTTTGATTCATGCTAAACAAATGGATAGCACACTAAAGAACATAACAATCCTCTCATAAAAGCCAGTTTTATTCAAGTCTCTTTCCGAAGTCAAGTAATAAGAGAAAATTACAGTAGAAAAAGAACTAAGGAGAAAGGATTCCTGATTCTACAGCTTCAACAACCTCCACCATACCACACCACTGGAAAATACTGCCCTCAAGGAGCTGATGGAACAATCTTGCCGGAGCATGTACCAAACCCGATAGTGTAACCATCTCCCTTGGAATATCCAGTTAAGGTGACTTCATCTCCATCTTCAAGAAATTTTCTATCTAACCCATTCAAAGACAGCGGATTTTGTCCATTCCATGTTAACTCTAGTAAGCATCCCCGAGACTCTAGCTCAGGGCCACTAATTGTCCCAGTTCCAAGAATGTCGCCTGGCCTCAGGTTGCAACCATTGATTGTGTGGTGAGCAAGTTGTTGAGTCAATGTCCAATATAAATGCTTGAGGTTACTTCGCGTAACAACACTTGAATCTTTGTGACCAGCAGGTTTAATGTGGACCTCTAGCGAAAGATCATAGTTTTTGGATACTTTTTCGGCCAAGTAAGGAAGTGGAGGAGGATCCTGTTTCGGGGCTTCACAAGCAAAAGGTTCTAATGCGTCCAAGGTCACAATCCAAGGTGATATCGATGTGGCGAAACTTTTTCCAAGAAAAGGACCAAGAGGAACATATTCCCATGCCTGAATATCTCGAGCACTCCAGTCATTCAGTAGAACAAGTCCAAAGATATGATCTTCAGCATTGTTTATATCTACAGGTTTTCCTAGTTCGTTTCCAGGTCCGACAATAGTAGCCATTTCCAACTCAAAGTCAAGCTTCAATGTAGGGCCAAAGTACGGTGAAGGGTTTCCGGCAGGATGAGCTTGACCCCTCGGTCGAATGATATCTGTTCCGGAAACAACAACAGAAGATGCCCGTCCATGATAAGCAACAGGCAAGTGATACCAATTCTCTAAAACAGGATTCTCCGGACCGCGAAATATGATTCCACAATTTTTAGTGTGATGCAGAGACGTAAAGAAGTCCGAATAGTCCCCAATTGCAACAGGGAGAAGCAATTCTACTTGGCTCAAAGGCACAAGTGATTTCTGCCTCAGAACATCATTGTCCCTCAAAGTTGGCTCAGTTGATGATAAGAGTTTTTGAAGAGTGGCACGAGCTTCCTTCCATGCTGGTCTTCCGAGTGATACAAACTTATTTAGATTAGGCTGGAGGAAGCAATCGGAGTTAGTTAGAAGAGTGCCGTTGAAAAGACCGGCGGAAGAGATCTCGGAGAGGTCGAGAACGAAATCGCCGATGGCTACGCCGGGACGAGGGGAAGAATCTGGTTGGGGCTTGAAGATTCCGTAAGGGAGATTCTGAATGGGGAAGTGAGAATCTGGGTGGACTTGGATGAAGGATTGAAGAAGAGTCATGGTGTTGACGGTTGCGTCGTGGTTGAGATGAAGTCAAGGATGGTAAGATGAAAGAGACAATAAACAGAGTGTTCTGTATAAAATGAATTNCTAGAGCTATAGAGAGAGAGTACTACTAATAAGTTGTCAATTCCCCGAA
TARGET=1601,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.9938
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=3.933921
PRIMER_LEFT_SEQUENCE=GGACTTGGATGAAGGATTGAAG
PRIMER_RIGHT_SEQUENCE=TTCGGGGAATTGACAACTTA
PRIMER_LEFT=1469,22
PRIMER_RIGHT=1638,20
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=58.066
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=3.9938
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=3.933921
PRIMER_LEFT_1_SEQUENCE=TTCTGAATGGGGAAGTGAGAAT
PRIMER_RIGHT_1_SEQUENCE=TTCGGGGAATTGACAACTTA
PRIMER_LEFT_1=1441,22
PRIMER_RIGHT_1=1638,20
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=58.066
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=3.9938
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=3.933921
PRIMER_LEFT_2_SEQUENCE=ATTCTGAATGGGGAAGTGAGAA
PRIMER_RIGHT_2_SEQUENCE=TTCGGGGAATTGACAACTTA
PRIMER_LEFT_2=1440,22
PRIMER_RIGHT_2=1638,20
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=58.066
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=4.2141
PRIMER_LEFT_3_PENALTY=0.280216
PRIMER_RIGHT_3_PENALTY=3.933921
PRIMER_LEFT_3_SEQUENCE=CTTGAAGATTCCGTAAGGGAGA
PRIMER_RIGHT_3_SEQUENCE=TTCGGGGAATTGACAACTTA
PRIMER_LEFT_3=1419,22
PRIMER_RIGHT_3=1638,20
PRIMER_LEFT_3_TM=59.720
PRIMER_RIGHT_3_TM=58.066
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=4.2245
PRIMER_LEFT_4_PENALTY=0.290589
PRIMER_RIGHT_4_PENALTY=3.933921
PRIMER_LEFT_4_SEQUENCE=TCGTGGTTGAGATGAAGTCAAG
PRIMER_RIGHT_4_SEQUENCE=TTCGGGGAATTGACAACTTA
PRIMER_LEFT_4=1516,22
PRIMER_RIGHT_4=1638,20
PRIMER_LEFT_4_TM=60.291
PRIMER_RIGHT_4_TM=58.066
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_03868 Ps_contig_mira-and-tgicl-ass_franssen_31331
SEQUENCE=ATCGATATATATTTATTATTAAAATTACACACCAAATACAAATAAAGAAAAATTACAACTTTTTGAAAAGATACATTTACAAGAATTTAATAACTTAAGATAATATAAGGATTTTATTTACAAATAGGAGTCTCATAAAAAATACAATAAACAAGCTATTAAAACATATATCTAATTATTGCAACAACAAATAATTAGATTATATGAAAAAAAATACAGTATTAATAGAAAAACTTAATAATAATAAAACATAAATGCTAGAAATTTTTGTTGGTAATATTTAACAAAACCACGATGATGAGAGTGTGATTCTGGCGAACGAACTCAGATAAATTCAAATAAACACTAAGTCATTTGATCGTTTTGTTTAACGTTATACGAACGAGGGAACGATTCCGTATCCAACTTCCGTAATTAGTGTTGTGGAAAACATTTGATCGACTTCAGCTTCAACGTCGGTCCGTTTCGCGAACCGACCTTTGATACGCGGCCGAGTTTCCGCGTAGGCTTTCCTCGAGGCGTACCGGATCGTTTTCTCGAATTTTCTTGTTTTCTTTTTTTCAAGGTACCTTAGGACTCTGGCTTCTCTGTCCATTGGAGAGAAATGAGAAGTCATTTGAATAGGAGGAGCTGAAAATAGGTCAATTGTTCCTTTTGAAGGTCTTGAATATGACATTGTTGTGGCATCTCTCATTGTTGATTCTGGTACAACACCAACATCCATTGATGAAACTGAAACACTTTGACTAATTGATCCACCATTGTAACTGAATCCAGCTTTGGAAGATTCAAACTCCAACCCAAGTTGAAAATTCTGTAGATGTTGCTGTTGAACAGGAACAACACTATCTCCAACATAGTTCTTTTGAGGAACACCATAATGATCTTGTCGCTGTTGTTGTTGATCACGACTATAGTCGTGATCATGAGTATTATTGGTAGTGAAAGTGTTTTCACCACCACCACAAGAATTACAATCCACAAGATCCAAATACTCATCAACTTCGCCGCTAAACAAAAATCCATTATTATTACCTTCTTGATTATGATCATTGCTATTGTTATTGTTGTTGTGGTTGTTGATATTGTGATGGTTGTTTTTCAAAGGATTCAACAAAAGCCATGAAGCTGCTTCATTTTCATCTTCCATATCAGCACCTTCATCCTCCTCTTCCTCCGCGTCACCGCCGCCGCGGACAAAGCCTTCGTCGTCAGCACCTAAAAGGGTCGTCGGAGGACCGTACAGGTATCCAGAAATCGGAAGAATCGGGACACGTTGGTGGCGGCTGGCAAGCGGGTTAGCGGAATGGATATCAGCGTCGCATGAGGAGCAGAGGGAAGCAGCGTCGGCTTTACAGAGAAAAGCCGCCGGGGCACGCTCGCAGGCCTCGCAGACCCAGACGCGTTCGTGCCTCGAGGCTACACGGTTTGCTGCGTGGATGCGAGCGTCGCATCCAGCGCATAAGTACGCTGAATCTGCGCGGCAGAATACCGCGCATGGAGCGGAGCGGCACGTGTCGCATGTCCGCGGCCATGTGACCGCGGACCGGACGGTGGCCGAGGTGGTGGTGAGGAATTTTTGCTCCAACATTTGTGGAGTGAGGGAGTGTGTGATGAATGGTTTGTTTGTCAGTTTTGGACAGACAGTGTTAAGGATGTTAAAATAGATGAAGTTTGTGTTTGCAGTGATTGTTCTTCCCCGAC
TARGET=236,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03869 Ps_contig_mira-and-tgicl-ass_franssen_31331
SEQUENCE=ATCGATATATATTTATTATTAAAATTACACACCAAATACAAATAAAGAAAAATTACAACTTTTTGAAAAGATACATTTACAAGAATTTAATAACTTAAGATAATATAAGGATTTTATTTACAAATAGGAGTCTCATAAAAAATACAATAAACAAGCTATTAAAACATATATCTAATTATTGCAACAACAAATAATTAGATTATATGAAAAAAAATACAGTATTAATAGAAAAACTTAATAATAATAAAACATAAATGCTAGAAATTTTTGTTGGTAATATTTAACAAAACCACGATGATGAGAGTGTGATTCTGGCGAACGAACTCAGATAAATTCAAATAAACACTAAGTCATTTGATCGTTTTGTTTAACGTTATACGAACGAGGGAACGATTCCGTATCCAACTTCCGTAATTAGTGTTGTGGAAAACATTTGATCGACTTCAGCTTCAACGTCGGTCCGTTTCGCGAACCGACCTTTGATACGCGGCCGAGTTTCCGCGTAGGCTTTCCTCGAGGCGTACCGGATCGTTTTCTCGAATTTTCTTGTTTTCTTTTTTTCAAGGTACCTTAGGACTCTGGCTTCTCTGTCCATTGGAGAGAAATGAGAAGTCATTTGAATAGGAGGAGCTGAAAATAGGTCAATTGTTCCTTTTGAAGGTCTTGAATATGACATTGTTGTGGCATCTCTCATTGTTGATTCTGGTACAACACCAACATCCATTGATGAAACTGAAACACTTTGACTAATTGATCCACCATTGTAACTGAATCCAGCTTTGGAAGATTCAAACTCCAACCCAAGTTGAAAATTCTGTAGATGTTGCTGTTGAACAGGAACAACACTATCTCCAACATAGTTCTTTTGAGGAACACCATAATGATCTTGTCGCTGTTGTTGTTGATCACGACTATAGTCGTGATCATGAGTATTATTGGTAGTGAAAGTGTTTTCACCACCACCACAAGAATTACAATCCACAAGATCCAAATACTCATCAACTTCGCCGCTAAACAAAAATCCATTATTATTACCTTCTTGATTATGATCATTGCTATTGTTATTGTTGTTGTGGTTGTTGATATTGTGATGGTTGTTTTTCAAAGGATTCAACAAAAGCCATGAAGCTGCTTCATTTTCATCTTCCATATCAGCACCTTCATCCTCCTCTTCCTCCGCGTCACCGCCGCCGCGGACAAAGCCTTCGTCGTCAGCACCTAAAAGGGTCGTCGGAGGACCGTACAGGTATCCAGAAATCGGAAGAATCGGGACACGTTGGTGGCGGCTGGCAAGCGGGTTAGCGGAATGGATATCAGCGTCGCATGAGGAGCAGAGGGAAGCAGCGTCGGCTTTACAGAGAAAAGCCGCCGGGGCACGCTCGCAGGCCTCGCAGACCCAGACGCGTTCGTGCCTCGAGGCTACACGGTTTGCTGCGTGGATGCGAGCGTCGCATCCAGCGCATAAGTACGCTGAATCTGCGCGGCAGAATACCGCGCATGGAGCGGAGCGGCACGTGTCGCATGTCCGCGGCCATGTGACCGCGGACCGGACGGTGGCCGAGGTGGTGGTGAGGAATTTTTGCTCCAACATTTGTGGAGTGAGGGAGTGTGTGATGAATGGTTTGTTTGTCAGTTTTGGACAGACAGTGTTAAGGATGTTAAAATAGATGAAGTTTGTGTTTGCAGTGATTGTTCTTCCCCGAC
TARGET=955,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7744
PRIMER_LEFT_PENALTY=0.175676
PRIMER_RIGHT_PENALTY=0.598735
PRIMER_LEFT_SEQUENCE=AATGATCTTGTCGCTGTTGTTG
PRIMER_RIGHT_SEQUENCE=GAGGAGGATGAAGGTGCTGATA
PRIMER_LEFT=879,22
PRIMER_RIGHT=1170,22
PRIMER_LEFT_TM=60.176
PRIMER_RIGHT_TM=60.599
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=0.8671
PRIMER_LEFT_1_PENALTY=0.095771
PRIMER_RIGHT_1_PENALTY=0.771376
PRIMER_LEFT_1_SEQUENCE=CACCAACATCCATTGATGAAAC
PRIMER_RIGHT_1_SEQUENCE=GCGAAGTTGATGAGTATTTGGA
PRIMER_LEFT_1=711,22
PRIMER_RIGHT_1=1007,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.229
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.9310
PRIMER_LEFT_2_PENALTY=0.332305
PRIMER_RIGHT_2_PENALTY=0.598735
PRIMER_LEFT_2_SEQUENCE=ATGATCTTGTCGCTGTTGTTGT
PRIMER_RIGHT_2_SEQUENCE=GAGGAGGATGAAGGTGCTGATA
PRIMER_LEFT_2=880,22
PRIMER_RIGHT_2=1170,22
PRIMER_LEFT_2_TM=59.668
PRIMER_RIGHT_2_TM=60.599
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.9450
PRIMER_LEFT_3_PENALTY=0.095771
PRIMER_RIGHT_3_PENALTY=0.849238
PRIMER_LEFT_3_SEQUENCE=CACCAACATCCATTGATGAAAC
PRIMER_RIGHT_3_SEQUENCE=GGCGAAGTTGATGAGTATTTGG
PRIMER_LEFT_3=711,22
PRIMER_RIGHT_3=1008,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=60.849
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.9471
PRIMER_LEFT_4_PENALTY=0.175676
PRIMER_RIGHT_4_PENALTY=0.771376
PRIMER_LEFT_4_SEQUENCE=AATGATCTTGTCGCTGTTGTTG
PRIMER_RIGHT_4_SEQUENCE=GCGAAGTTGATGAGTATTTGGA
PRIMER_LEFT_4=879,22
PRIMER_RIGHT_4=1007,22
PRIMER_LEFT_4_TM=60.176
PRIMER_RIGHT_4_TM=59.229
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=129
=
PRIMER_SEQUENCE_ID=SSR_03870 Ps_contig_mira-and-tgicl-ass_franssen_31332
SEQUENCE=GCCGGGGCGGGGGAGTAAGTAAGACTACTAAGTAGTTCGACTAAATAAGTGTTCTTTACACGACCATTCTTTCATTTTCCCGGCCAAGTTTAGNAGTCGGGGACTCCAATTCTTCAAACCCATAATTAACTATTCCTAAACCCAAAAGGCCCTTTTACCTATACATACTTTACATTTTCCATATGCACCTAACATCATAAGAAACTCATCTAATCCTTACAACTCTCATATCAAACCATAACCAAACATCATTAATTCCCTTCAAAACCATACATGGATCCTCAAAGTAAACTACAACTAAGGATCCTTTATTGGATCTTATTACTTGTTTGTGTTACTAATACTCTTCCAATCAAAGCTTTAGAACCAAGGAAGCTAGTTGAGAAGTGCACACCATGTGGTGACAATCCAATATCATCACCACCTCCAATAATATACAATTCACCACCACCAATTGTGTATTATCCATCACCACCACCACCATCTCCGCCACCTCCAATAGTATACTACTCTCCACCACCACCTTCTCCGAAGAAATCACCCTCCTCGAATTGTCCTCCCCCACCGTCGTCGCCATACATATACATGACCGGACCGCCGGGGAACTTGTATCCAGTTGATGTGAATTTCAGTGGCTCAGGAGCAACTCCAGCCGGCCGTGGAAGCTTTGCAGGATTCTTGCCACTGCTGGTTTGTTTGTTGAGCTTTCTTGCCTTTTGGTGATTAGTTTGAGATTAAAATACTCAAAATATGAGTAGAAGATAATACATATATATAAGGTAATGGGGAAGAGAAATTATATTAGTGTTTAATATGTTCTATATGTGATCCCAATTTGTTGATCATATATATATTTATTTCCATGTCTACTTTTCTTCATGTGTAGTTTCGTTGATCCAATAAACTTTCTTCTGTACCATAGAGAAAGTAATAAATTTATGAAGTCTTTTCTTAAGCTTGAATGTAACTTTTAATTTAGATTATGCATCACATCTGTACTCAAGAAAATTTCCATTCGAACTTAATCTTGGACACAATTTTCTCC
TARGET=470,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0816
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=TTCACCACCACCAATTGTGTAT
PRIMER_RIGHT_SEQUENCE=AAGGCAAGAAAGCTCAACAAAC
PRIMER_LEFT=440,22
PRIMER_RIGHT=715,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.1331
PRIMER_LEFT_1_PENALTY=0.015253
PRIMER_RIGHT_1_PENALTY=0.117817
PRIMER_LEFT_1_SEQUENCE=TTCACCACCACCAATTGTGTAT
PRIMER_RIGHT_1_SEQUENCE=GAGCCACTGAAATTCACATCAA
PRIMER_LEFT_1=440,22
PRIMER_RIGHT_1=637,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=60.118
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.1402
PRIMER_LEFT_2_PENALTY=0.022341
PRIMER_RIGHT_2_PENALTY=0.117817
PRIMER_LEFT_2_SEQUENCE=ACCATGTGGTGACAATCCAATA
PRIMER_RIGHT_2_SEQUENCE=GAGCCACTGAAATTCACATCAA
PRIMER_LEFT_2=392,22
PRIMER_RIGHT_2=637,22
PRIMER_LEFT_2_TM=59.978
PRIMER_RIGHT_2_TM=60.118
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=0.2082
PRIMER_LEFT_3_PENALTY=0.090376
PRIMER_RIGHT_3_PENALTY=0.117817
PRIMER_LEFT_3_SEQUENCE=ATCCAATATCATCACCACCTCC
PRIMER_RIGHT_3_SEQUENCE=GAGCCACTGAAATTCACATCAA
PRIMER_LEFT_3=406,22
PRIMER_RIGHT_3=637,22
PRIMER_LEFT_3_TM=59.910
PRIMER_RIGHT_3_TM=60.118
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=232
PRIMER_PAIR_PENALTY_4=0.2464
PRIMER_LEFT_4_PENALTY=0.022341
PRIMER_RIGHT_4_PENALTY=0.224014
PRIMER_LEFT_4_SEQUENCE=ACCATGTGGTGACAATCCAATA
PRIMER_RIGHT_4_SEQUENCE=GTATACTATTGGAGGTGGCGGA
PRIMER_LEFT_4=392,22
PRIMER_RIGHT_4=506,22
PRIMER_LEFT_4_TM=59.978
PRIMER_RIGHT_4_TM=60.224
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_03871 Ps_contig_mira-and-tgicl-ass_franssen_31332
SEQUENCE=GCCGGGGCGGGGGAGTAAGTAAGACTACTAAGTAGTTCGACTAAATAAGTGTTCTTTACACGACCATTCTTTCATTTTCCCGGCCAAGTTTAGNAGTCGGGGACTCCAATTCTTCAAACCCATAATTAACTATTCCTAAACCCAAAAGGCCCTTTTACCTATACATACTTTACATTTTCCATATGCACCTAACATCATAAGAAACTCATCTAATCCTTACAACTCTCATATCAAACCATAACCAAACATCATTAATTCCCTTCAAAACCATACATGGATCCTCAAAGTAAACTACAACTAAGGATCCTTTATTGGATCTTATTACTTGTTTGTGTTACTAATACTCTTCCAATCAAAGCTTTAGAACCAAGGAAGCTAGTTGAGAAGTGCACACCATGTGGTGACAATCCAATATCATCACCACCTCCAATAATATACAATTCACCACCACCAATTGTGTATTATCCATCACCACCACCACCATCTCCGCCACCTCCAATAGTATACTACTCTCCACCACCACCTTCTCCGAAGAAATCACCCTCCTCGAATTGTCCTCCCCCACCGTCGTCGCCATACATATACATGACCGGACCGCCGGGGAACTTGTATCCAGTTGATGTGAATTTCAGTGGCTCAGGAGCAACTCCAGCCGGCCGTGGAAGCTTTGCAGGATTCTTGCCACTGCTGGTTTGTTTGTTGAGCTTTCTTGCCTTTTGGTGATTAGTTTGAGATTAAAATACTCAAAATATGAGTAGAAGATAATACATATATATAAGGTAATGGGGAAGAGAAATTATATTAGTGTTTAATATGTTCTATATGTGATCCCAATTTGTTGATCATATATATATTTATTTCCATGTCTACTTTTCTTCATGTGTAGTTTCGTTGATCCAATAAACTTTCTTCTGTACCATAGAGAAAGTAATAAATTTATGAAGTCTTTTCTTAAGCTTGAATGTAACTTTTAATTTAGATTATGCATCACATCTGTACTCAAGAAAATTTCCATTCGAACTTAATCTTGGACACAATTTTCTCC
TARGET=845,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.4625
PRIMER_LEFT_PENALTY=0.066394
PRIMER_RIGHT_PENALTY=2.396117
PRIMER_LEFT_SEQUENCE=GTTTGTTGAGCTTTCTTGCCTT
PRIMER_RIGHT_SEQUENCE=GGATCAACGAAACTACACATGA
PRIMER_LEFT=694,22
PRIMER_RIGHT=897,22
PRIMER_LEFT_TM=59.934
PRIMER_RIGHT_TM=57.604
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=3.4687
PRIMER_LEFT_1_PENALTY=1.072607
PRIMER_RIGHT_1_PENALTY=2.396117
PRIMER_LEFT_1_SEQUENCE=CTGGTTTGTTTGTTGAGCTTTC
PRIMER_RIGHT_1_SEQUENCE=GGATCAACGAAACTACACATGA
PRIMER_LEFT_1=687,22
PRIMER_RIGHT_1=897,22
PRIMER_LEFT_1_TM=58.927
PRIMER_RIGHT_1_TM=57.604
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=3.6437
PRIMER_LEFT_2_PENALTY=1.247566
PRIMER_RIGHT_2_PENALTY=2.396117
PRIMER_LEFT_2_SEQUENCE=GTGAATTTCAGTGGCTCAGGA
PRIMER_RIGHT_2_SEQUENCE=GGATCAACGAAACTACACATGA
PRIMER_LEFT_2=621,21
PRIMER_RIGHT_2=897,22
PRIMER_LEFT_2_TM=60.248
PRIMER_RIGHT_2_TM=57.604
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=3.6926
PRIMER_LEFT_3_PENALTY=1.296491
PRIMER_RIGHT_3_PENALTY=2.396117
PRIMER_LEFT_3_SEQUENCE=TGTTTGTTGAGCTTTCTTGCCT
PRIMER_RIGHT_3_SEQUENCE=GGATCAACGAAACTACACATGA
PRIMER_LEFT_3=693,22
PRIMER_RIGHT_3=897,22
PRIMER_LEFT_3_TM=61.296
PRIMER_RIGHT_3_TM=57.604
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=3.8096
PRIMER_LEFT_4_PENALTY=1.413531
PRIMER_RIGHT_4_PENALTY=2.396117
PRIMER_LEFT_4_SEQUENCE=TGTTTGTTGAGCTTTCTTGCC
PRIMER_RIGHT_4_SEQUENCE=GGATCAACGAAACTACACATGA
PRIMER_LEFT_4=693,21
PRIMER_RIGHT_4=897,22
PRIMER_LEFT_4_TM=60.414
PRIMER_RIGHT_4_TM=57.604
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_03872 Ps_contig_mira-and-tgicl-ass_franssen_31335
SEQUENCE=TTCGGGTTAATTCTTACTATTTGNACTACTACGAGCGTTTTTGTTCTCGTGTCAACTTCTTACAGCATTTCCACATATCAACGAGTATTAAACACCACACTCTCTCATCATTCAGACATTCATCACCTTCTTCTCGCTCTCTTCTCTCCACACACACATAGCCTCTCAATCGCGTCTTCCTTTCTCTCTACTCTCTCGAACCTTCCTCGCCAATGGCTGCCACCGCTACCGCTACCAACCTCGACAATCTCAAATCCTCCGTCGCTGGATTGGAGCAAATCAGTGAGAATGAGAAAAACGGATTCATCAACCTCGTCTCTCGTTATCTCAGCGGCGAAGCGCAACATGTAGAATGGAGTAAGATCCAGACGCCAACTGATGAAGTGGTTGTGCCTTATGACACTTTGGCGCCAACTCCTGCTGGTTCTTCAGAGATTAAGAATCTTTTGGATAAGCTTGTGGTGTTGAAGCTTAATGGAGGCTTGGGGACAACTATGGGTTGCACTGGTCCAAAGTCCGTCATTGAAGTTCGTGATGGACTGACATTTTTAGATTTGATTGTCATCCAAATTGAGAATCTCAATTCCAAATATGGGAGCAGTGTTCCTTTGCTTTTGATGAACTCATTCAACACTCATGACGACACTCAAAAGATTGTTGAAAAATACCAAAACTCGAATATTGAGATTCATACTTTTAACCAGAGCCAATATCCTCGATTGATTGCTGATGACTTTTTACCTTTGCCTTCCAAAGGGAACACTGGCAAGGATGGGTGGTATCCTCCTGGTCATGGGGATGTCTTCCCATCATTATCGAACAGTGGAAAACTTGATGCATTATTATCACAGGGTAAAGAGTATGTGTTTGTTGCCAATTCAGATAATTTGGGAGCTATAGTTGACTTGAAAATCTTAAACCATCTGATCGAGCACAAGAATGAATATTGTATGGAGGTAACTCCCAAAACATTGGCCGATGTTAAGGGTGGCACTTTGATTTCTTATGAAGGAAGGGTTCAGCTCCTGGAAATTGCACAAGTCGCAGATGAACATGTTGGGGAATTCAAGTCAATTGAGAAGTTCAAAATTTTCAACACAAATAATTTGTGGGTGAACTTGAAAGCAATCAAAAGACTTGTTGAAGCCGATGCTCTTAAGATGGAGATTATTCCTAATCCAAAGGAAGTTGATGGAGTAAAAGTTCTTCAGCTGGAAACTGCAGCTGGTGCAGCAATAAGGTTCTTTGACAAGGCTATTGGGATTAATGTTCCTCGATCCCGATTCCTTCCGGTGAAGGCAACTTCAGATTTGCTTCTTGTCCAGTCAGACCTCTACACCTTGGAAGATGGATCTGTCATTCGGAACCCAGCTAGGGCTAATCCTGAAAACCCCGCTATTGAACTGGGGCCTGAATTTAAGAAGGTTAGCAACTTCTTGAGCCGCTTCAAGTCAATTCCCAGTATTGTTGAGCTTGACAGTGTAAAAGTGGCTGGCGATGTATGGTTTGGAGCTGGTGTCATCCTCAAGGGGAAAATCAGTATCGTAGCCAAATCTGGCGTAAAGTTGGAAATTCCTGACGGAGCCGTAATTGCAAACAAGGAAATTAATGGTCCGGAGGACCTTTGAGGAAGCTGATGAGTTTAAATGCATAGGACTGTATACAGTATAATGGCAGTCTTGTGTTACTTCAATAATGTTTTACCCAAGTTTGTTAGGAAAAGGAAATAAGAAGGCGCTTTTGGTCGTGAGTCTGTCTCAGTATGAGGCCTTGCTGGTTGTCTTCTTACCGTTACATAAGTTGTCATGCCATTTTGTGTCACATAGATTTTCATGCCATTTTGTGTAACAATGGTATCCATCTGATTTTGTCATCTTTTCTGTCTTATATACATCCCCTCTCCTTAAAAAAAAGGCTTAGTGCTACAATGTACTACATCTACATCTGTCTCATTATAAGTTTTTGTTTGGAAAGGTAAAGTATATTAAACATCC
TARGET=149,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1827
PRIMER_LEFT_PENALTY=0.145354
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=ATTCATCACCTTCTTCTCGCTC
PRIMER_RIGHT_SEQUENCE=TGTCATAAGGCACAACCACTTC
PRIMER_LEFT=117,22
PRIMER_RIGHT=401,22
PRIMER_LEFT_TM=59.855
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.1827
PRIMER_LEFT_1_PENALTY=0.145354
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=ATTCATCACCTTCTTCTCGCTC
PRIMER_RIGHT_1_SEQUENCE=GTCATAAGGCACAACCACTTCA
PRIMER_LEFT_1=117,22
PRIMER_RIGHT_1=400,22
PRIMER_LEFT_1_TM=59.855
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=0.1905
PRIMER_LEFT_2_PENALTY=0.153161
PRIMER_RIGHT_2_PENALTY=0.037339
PRIMER_LEFT_2_SEQUENCE=CATCACCTTCTTCTCGCTCTCT
PRIMER_RIGHT_2_SEQUENCE=TGTCATAAGGCACAACCACTTC
PRIMER_LEFT_2=120,22
PRIMER_RIGHT_2=401,22
PRIMER_LEFT_2_TM=60.153
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.1905
PRIMER_LEFT_3_PENALTY=0.153161
PRIMER_RIGHT_3_PENALTY=0.037339
PRIMER_LEFT_3_SEQUENCE=CATCACCTTCTTCTCGCTCTCT
PRIMER_RIGHT_3_SEQUENCE=GTCATAAGGCACAACCACTTCA
PRIMER_LEFT_3=120,22
PRIMER_RIGHT_3=400,22
PRIMER_LEFT_3_TM=60.153
PRIMER_RIGHT_3_TM=60.037
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.2408
PRIMER_LEFT_4_PENALTY=0.110942
PRIMER_RIGHT_4_PENALTY=0.129818
PRIMER_LEFT_4_SEQUENCE=ACAGCATTTCCACATATCAACG
PRIMER_RIGHT_4_SEQUENCE=ATTTGAGATTGTCGAGGTTGGT
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=254,22
PRIMER_LEFT_4_TM=59.889
PRIMER_RIGHT_4_TM=59.870
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_03873 Ps_contig_mira-and-tgicl-ass_franssen_31338
SEQUENCE=GAAATTAAAGACACTGCTATTATATAATCAGATTTTATCTAGGGATGAGARRCNACTTACTTCTTCCTAGAAAGAAATTAAAAGAGCTCTAAAAACCTCATTTCTTTTTCTTTTCATTTATCAAACTTTTAATGCTGAACCAGATGTCAGGACAGACTCATTTATTGAGTCATGTAAGTATAGGTTGAATTTAAGTGCTTTTTTTTTCTTACTTACTTAACCTCTTCTCTCTGTCTTAGCAGCCTCAAATTTTGACCACTTATCACACTTTTCATCATCACTTAGTTTTTAACTATAAAACAAGAAAGTTAGTTTGAGGAATTCTCTTGTATTGTATTGTAGTGTGAGTTGAGAGATGAGTTATTTGGGTGTAGGTATTAGTCCTGGAACTGTTCCTGTGTATCACAGCACAGACATGAAAGTGTTTGATAGAAAGATTAGGATAACTGAGTTGGTTTTGAGGTGTGTGATTCTTGGTTTAGGTGTTCTTGCTGTTGTTCTTATTGTGACTGATTCACAAGTCAGAGTGTTTTTCACACTTGAGAAGAAAGCTAAATTCACTGACATGAAGGCTTTAGTGTTTCTGGTTGTTGCAAATGCGTTAGCTGCTGGTTATTCCTTAATCCAAGGGCTAAGGTGTGTAGTGAGTATGATTAAAGGAAGTGTACTCTTCAACAAGCCCTTAGCTTGGGCCATTTTTTCGGGTGATCAGGTAATGGCATACATAACAGTGTCTGCGGTGGCGGCCGCAGCACAATCTGCAGTGTTTGCAAAGATGGGTCAGCCAGAGTTACAATGGATGAAGATATGCAACATGTATGGAAAATTCTGCAACCAAGTTGGAGAAGGGTTAGCTAGTGCATTTTTGGTTAGCCTAAGTATGGTTATACTATCATGTATTTCAGCTTTCAGTCTGTTCCGTTTGTATGGTGGTAGCAAAACTAAAAATGCATATTGGTAGGTTAGAATATAAGTTAAAATATAATAGTTTGAAAGTGAATAAATAAAACCTACCACTCAAAAAACTAGTACTGTTTTCTTGGTTTGCAATTTACAAGGCGTTTTGGTGTATATGTATGTAGTAGTATAGTAGTGAATGATGATTGTGATATATGAATATGACTTATGAGAATGCAGTTATTGTAATGATCNG
TARGET=208,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03874 Ps_contig_mira-and-tgicl-ass_franssen_31338
SEQUENCE=GAAATTAAAGACACTGCTATTATATAATCAGATTTTATCTAGGGATGAGARRCNACTTACTTCTTCCTAGAAAGAAATTAAAAGAGCTCTAAAAACCTCATTTCTTTTTCTTTTCATTTATCAAACTTTTAATGCTGAACCAGATGTCAGGACAGACTCATTTATTGAGTCATGTAAGTATAGGTTGAATTTAAGTGCTTTTTTTTTCTTACTTACTTAACCTCTTCTCTCTGTCTTAGCAGCCTCAAATTTTGACCACTTATCACACTTTTCATCATCACTTAGTTTTTAACTATAAAACAAGAAAGTTAGTTTGAGGAATTCTCTTGTATTGTATTGTAGTGTGAGTTGAGAGATGAGTTATTTGGGTGTAGGTATTAGTCCTGGAACTGTTCCTGTGTATCACAGCACAGACATGAAAGTGTTTGATAGAAAGATTAGGATAACTGAGTTGGTTTTGAGGTGTGTGATTCTTGGTTTAGGTGTTCTTGCTGTTGTTCTTATTGTGACTGATTCACAAGTCAGAGTGTTTTTCACACTTGAGAAGAAAGCTAAATTCACTGACATGAAGGCTTTAGTGTTTCTGGTTGTTGCAAATGCGTTAGCTGCTGGTTATTCCTTAATCCAAGGGCTAAGGTGTGTAGTGAGTATGATTAAAGGAAGTGTACTCTTCAACAAGCCCTTAGCTTGGGCCATTTTTTCGGGTGATCAGGTAATGGCATACATAACAGTGTCTGCGGTGGCGGCCGCAGCACAATCTGCAGTGTTTGCAAAGATGGGTCAGCCAGAGTTACAATGGATGAAGATATGCAACATGTATGGAAAATTCTGCAACCAAGTTGGAGAAGGGTTAGCTAGTGCATTTTTGGTTAGCCTAAGTATGGTTATACTATCATGTATTTCAGCTTTCAGTCTGTTCCGTTTGTATGGTGGTAGCAAAACTAAAAATGCATATTGGTAGGTTAGAATATAAGTTAAAATATAATAGTTTGAAAGTGAATAAATAAAACCTACCACTCAAAAAACTAGTACTGTTTTCTTGGTTTGCAATTTACAAGGCGTTTTGGTGTATATGTATGTAGTAGTATAGTAGTGAATGATGATTGTGATATATGAATATGACTTATGAGAATGCAGTTATTGTAATGATCNG
TARGET=327,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03875 Ps_contig_mira-and-tgicl-ass_franssen_31339
SEQUENCE=GGGGGGTCTTCGTCTCTACTGATAACAACTCGCGCCTCTATCAGAGTCTTGTTACTCCTACCCGACCGAAACCATGGCTCCAAAGAAGGAAAAGGCCCCTCCACCTTCCTCCAAGCCAGCAAAATCTGGTGGTGGCAAGCAGAAGAAGAAGAAGTGGAGCAAAGGAAAGCAAAAGGAGAAGGTGAACAACATGGTCCTGTTTGATCAGGGTACCTATGACAAGCTCCTAACTGAAGCACCCAAATACAAGCTCATTACTCCTTCAATTCTCTCTGATCGTTTGAGGGTTAATGGGTCACTTGCAAGGAGGGCTATAAGGGACTTGATGGCTAAAGGTTTAATCAGGTTGGTATCTGCCCATGCAAGTCAGCAGATTTACACCAGGGCTACCAACACCTAAGTGTAGCAGAGAGAGAAAGTATCAGTAGAGGCTTTTTATGTATTGTTCTTTCCAATTTTGTGATGCACATTATACAAGTCTGTATTAGTTCTGCTGAATTTATAGGCTGAGTATTTTTTTTGTATCCTTGTATTTTTGGCATCTATCTTATTGTTTTTTTTGGTATTCTGGTATGTCTTGGTCCCAATTTTATTTCATTGGATCTCCATTCTGAAAAATGATTTGATTTAGGTAAC
TARGET=141,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0616
PRIMER_LEFT_PENALTY=0.046672
PRIMER_RIGHT_PENALTY=0.014945
PRIMER_LEFT_SEQUENCE=GCGCCTCTATCAGAGTCTTGTT
PRIMER_RIGHT_SEQUENCE=AAGTCCCTTATAGCCCTCCTTG
PRIMER_LEFT=31,22
PRIMER_RIGHT=323,22
PRIMER_LEFT_TM=60.047
PRIMER_RIGHT_TM=59.985
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.0616
PRIMER_LEFT_1_PENALTY=0.046672
PRIMER_RIGHT_1_PENALTY=0.014945
PRIMER_LEFT_1_SEQUENCE=GCGCCTCTATCAGAGTCTTGTT
PRIMER_RIGHT_1_SEQUENCE=CAAGTCCCTTATAGCCCTCCTT
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=324,22
PRIMER_LEFT_1_TM=60.047
PRIMER_RIGHT_1_TM=59.985
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=0.0744
PRIMER_LEFT_2_PENALTY=0.046672
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=GCGCCTCTATCAGAGTCTTGTT
PRIMER_RIGHT_2_SEQUENCE=ATGAGCTTGTATTTGGGTGCTT
PRIMER_LEFT_2=31,22
PRIMER_RIGHT_2=254,22
PRIMER_LEFT_2_TM=60.047
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.0744
PRIMER_LEFT_3_PENALTY=0.046672
PRIMER_RIGHT_3_PENALTY=0.027715
PRIMER_LEFT_3_SEQUENCE=GCGCCTCTATCAGAGTCTTGTT
PRIMER_RIGHT_3_SEQUENCE=AATGAGCTTGTATTTGGGTGCT
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=60.047
PRIMER_RIGHT_3_TM=60.028
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=0.0971
PRIMER_LEFT_4_PENALTY=0.046672
PRIMER_RIGHT_4_PENALTY=0.050443
PRIMER_LEFT_4_SEQUENCE=GCGCCTCTATCAGAGTCTTGTT
PRIMER_RIGHT_4_SEQUENCE=GGTGCTTCAGTTAGGAGCTTGT
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=239,22
PRIMER_LEFT_4_TM=60.047
PRIMER_RIGHT_4_TM=59.950
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_03876 Ps_contig_mira-and-tgicl-ass_franssen_31341
SEQUENCE=ATCTGAATCTCAACTTGCAGAAARGAATAAATTCTGTTTGGGAATCGTAAATAGTTCCTTTCCGATCTCAATAAGATAAACTGGTTCGGTTTGTTCTTGTAACTTCCGCTTTCTCTCTCTCTCTCTTCATAGTTCATAAAGTAACCTAAATTTTGAGAGAGAGAGGAATAATAATAGTATGACAAGAAAGAAGATACAGATCAAGAAAATCGATAACATCAGTTCAAGGCAAGTGACTTTCTCTAAGAGGAGGAAAGGGCTTTTCAAGAAAGCTCAGGAGCTTTCAACTCTCTGTGATGCAGATGTAGCTCTTATGGTTTTTTCTGCAACTAATAAGCTCTTTGAGTATGCTAGTTCCAGTATGCAACAAGTAATTGAAAGGCGTAATGGATGTTCTGAAAATCATAGATTAATGGACCGTCCATCTACGGATCAGTTTCAGGTTGAGAGTGAGTCCAGCGACACACTGCACAAGAAATTAGAAGATAAATCTCGTGAACTGAGACAAATGAATGGAGAAGATTTGCAAGAATTGACAGTACAAGAACTCCAGAAACTAGAGACTCTACTTAGAAGAAGTTTGTCCAGTGTCTCAAAAAAGAAGGATGAAATGTTTATTCAAGGCATCAACACCCTTAAGAGAAAGGAAGTGGAACTGATCCGAGAAAATCAAAGACTGAAGCATGTTGTACCAGACCTTATAATTGGACAAAGGCAACAATCATTGGAATCGGTTATTAGACGTTCATCTTATTTTCTTGAAGACGGTGGTGACAGTGACACTTCTCTCAAGTTAGGGTTACCATTTCATAATTAAATAAATGAAGTTTGGTTATAGGACTTTGAGAAGAAGATAACAGTTTCGCTTCCTTTCTATGTCGCTGAATAACAGTAACTTGCTATTTAAAAATAAGTCATGTAAAGTAATAAAATCTAGCTAAGTAATGTGAGGTCTTGTTTGTAGAATTGTAATTGTTCTATATTCTTATGGAAAATTGATTCACCTAAATGTTTATATTTATTATCGAGTAGATGTAGTCCACCAATCACTATTATATAACTTACAAAAATACTTGAAACAATATTAGAAGTTAAATTTT
TARGET=112,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03877 Ps_contig_mira-and-tgicl-ass_franssen_31341
SEQUENCE=ATCTGAATCTCAACTTGCAGAAARGAATAAATTCTGTTTGGGAATCGTAAATAGTTCCTTTCCGATCTCAATAAGATAAACTGGTTCGGTTTGTTCTTGTAACTTCCGCTTTCTCTCTCTCTCTCTTCATAGTTCATAAAGTAACCTAAATTTTGAGAGAGAGAGGAATAATAATAGTATGACAAGAAAGAAGATACAGATCAAGAAAATCGATAACATCAGTTCAAGGCAAGTGACTTTCTCTAAGAGGAGGAAAGGGCTTTTCAAGAAAGCTCAGGAGCTTTCAACTCTCTGTGATGCAGATGTAGCTCTTATGGTTTTTTCTGCAACTAATAAGCTCTTTGAGTATGCTAGTTCCAGTATGCAACAAGTAATTGAAAGGCGTAATGGATGTTCTGAAAATCATAGATTAATGGACCGTCCATCTACGGATCAGTTTCAGGTTGAGAGTGAGTCCAGCGACACACTGCACAAGAAATTAGAAGATAAATCTCGTGAACTGAGACAAATGAATGGAGAAGATTTGCAAGAATTGACAGTACAAGAACTCCAGAAACTAGAGACTCTACTTAGAAGAAGTTTGTCCAGTGTCTCAAAAAAGAAGGATGAAATGTTTATTCAAGGCATCAACACCCTTAAGAGAAAGGAAGTGGAACTGATCCGAGAAAATCAAAGACTGAAGCATGTTGTACCAGACCTTATAATTGGACAAAGGCAACAATCATTGGAATCGGTTATTAGACGTTCATCTTATTTTCTTGAAGACGGTGGTGACAGTGACACTTCTCTCAAGTTAGGGTTACCATTTCATAATTAAATAAATGAAGTTTGGTTATAGGACTTTGAGAAGAAGATAACAGTTTCGCTTCCTTTCTATGTCGCTGAATAACAGTAACTTGCTATTTAAAAATAAGTCATGTAAAGTAATAAAATCTAGCTAAGTAATGTGAGGTCTTGTTTGTAGAATTGTAATTGTTCTATATTCTTATGGAAAATTGATTCACCTAAATGTTTATATTTATTATCGAGTAGATGTAGTCCACCAATCACTATTATATAACTTACAAAAATACTTGAAACAATATTAGAAGTTAAATTTT
TARGET=155,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03878 Ps_contig_mira-and-tgicl-ass_franssen_31347
SEQUENCE=AACTCAACTAATGCGAATTTGAATTACACTTTAATAAAATTAATATACAATTTTGTATGCAATTTTACATTAAAGATACAAATTAAAAACACACAAAATAACTCGTCATCTTTTCCCCAAGCCCATAAGGCATGAAAGTTTAAAGAATCTAGCAAATAATAGAAATTATGCAGCCAAAAAATACAGCAATGTTTTAATGGCCTATTTTTATTTTAGTTTTTGGTCTGTATTTAATGGCCTATTTTTATTTTAGTTTTTGGTCTGTATTTAATGGCCTATTTAATAATATTATTATAGTATACATTTATTCCATTCCACATTTTATTTCAATCCAATTATTAAATTGGTGAATTACTAAATTTCACATTCTGGCCTTAGCAACCACCATGCTGCCATTGCCTCCATCAATTCCCACTGCTCCTGGCCTAGTCCATGTTTCCTTAAATTGCTCTTCAGTCACTCCTTTCCCCATTGCCGCTGTTGATGCAACACCTCCTTGGATAGTTTTGAGGTATCTATCATAGTAATTAGCACCAGCCCATTTCTGATGAGCAAGTGTATCAACACCATTCTTCCTTTCCTCTCTTTGAATCCTTTCAACATAAGCCAACATTCCCCTGCTAGCAAAATCCTTAGCAAACGTTGAAGTGATTAATGCATTACAATGCAAACCACCAACAGTTATAAACTGCCAAACATATCCTAACTTAGCAATCTTAGGAATGAAATCTCTCATTTCCACATCACTCATGCCAGAAGCATCCCAATTAAACGACGGCGAAAGATTGTAACCAAGCATCATCTCTGGATGCTTTGATCTCACCCCTTCAGCAAACTCTGTGCATTCAGCCACATCTGGACTCGCTGTTTCCATCCATATAAGATCCGCGTGTGGCGCGAAAGCGCAACCGCGAACAACAGCCGCGGTAACAGAGCCTTTAAACCTGTAAAACCCTTCTCTTGTTCTAGGTAAGTCCCAATCCCAAAACAAGCTCCTCACACCTAATCTCTCAGCAATTTCCCTTCCTTCATCAATTGAAAGACACTTTTCGTAACTCGAAAAATGCTTCCACTCGTTCAACTTCCTCGTTTTCTCGTCCTCGTTAGCATTTTGATTCTTAATTGCATCCGTTACTGCTTCTGAGAAAGTCTTCAGTTGCGCATTCGACAACCAATGATCCTCCAATGCCTGAAGCTCGGATCCACTCTTGCCAGCCGCGAATCCCTGTGCCATAACTGTGACTAAACTCTTCCACTTCATATTCGGATTCGTCACGCCCAAAATAAACTGATGATCTCTAGTATCGATATTCGATTGGATAAGATTAGCAGCAACAGCGTCTGTTCTAGCAACCAAAACAGTTTCAACCCCCATAACATCAAACTGAAGCCTAGCAGCAACAAGCCTGTTAATATGTTCACTAACCGCAACCAACACTTTCCCCGCCATGTGACCGCATTTTTTCGTCACAGAAGATTGATCCTCGATATGAACACCAGCAGCACCGCGTTCGACGAAAAGCTTACAAAGCTTAACAGTTGCTGTCGTGCCGCCAAAGCCAGTATCACCGTCAGCTATAATTGGTAACTCAAGTAATCAACAAAAGGCGTCCGTGCTCGTTCTTCTCGTCTCATATTCATCCTCTCTTCTTTTTGTTTTCTGTCATGATACAGTTGAGCAAAGAAAAGATGTTCAACTTTGTTCGGGACAGTGTCGTAAGGATAATCAGCGAGATCAGGACCGGGTTCATTTGTTGTTGTGTGTGTGGATGAACACTGCCAACCGGAGACGTAAATTGTGTCTAAATATTTTGCCATTTGAGTGACTTGAACCGGATCAAGTGCACCAAATGTTCTTGATGCTGTTTTGTTGGTTTGGTGAGTTTTGAGAAGATTCCAGAGCTTTTTGGATAACTCGTTTGAGACATAGGTTTGTCTTAGGTTTCCTCTTAAGGAAACTACATCTTTAGCTGAATATGATCTTTTGGTTAGTTTGGAATCTCTCAGAGTTCCACCATGCTTGTACCTCTGATACTTCGGATTCGAATCTTGCTTCTTCTTCCGCTAGAGATGGAGGCACAAAATTTGAAGAGGCCATGGTTAGAACTTTGAACTCTTTCTTTTTTTAACGGTAGATGTTACTTACACACAACTAAGTGAAGCTCATGCCTACTCTGCGTTGA
TARGET=1759,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1045
PRIMER_LEFT_PENALTY=0.067187
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=GGATAATCAGCGAGATCAGGAC
PRIMER_RIGHT_SEQUENCE=ACAGCATCAAGAACATTTGGTG
PRIMER_LEFT=1720,22
PRIMER_RIGHT=1865,22
PRIMER_LEFT_TM=60.067
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=0.1045
PRIMER_LEFT_1_PENALTY=0.067187
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=GATAATCAGCGAGATCAGGACC
PRIMER_RIGHT_1_SEQUENCE=ACAGCATCAAGAACATTTGGTG
PRIMER_LEFT_1=1721,22
PRIMER_RIGHT_1=1865,22
PRIMER_LEFT_1_TM=60.067
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=0.1123
PRIMER_LEFT_2_PENALTY=0.067187
PRIMER_RIGHT_2_PENALTY=0.045112
PRIMER_LEFT_2_SEQUENCE=GGATAATCAGCGAGATCAGGAC
PRIMER_RIGHT_2_SEQUENCE=CTCAAAACTCACCAAACCAACA
PRIMER_LEFT_2=1720,22
PRIMER_RIGHT_2=1889,22
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=60.045
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.1123
PRIMER_LEFT_3_PENALTY=0.067187
PRIMER_RIGHT_3_PENALTY=0.045112
PRIMER_LEFT_3_SEQUENCE=GATAATCAGCGAGATCAGGACC
PRIMER_RIGHT_3_SEQUENCE=CTCAAAACTCACCAAACCAACA
PRIMER_LEFT_3=1721,22
PRIMER_RIGHT_3=1889,22
PRIMER_LEFT_3_TM=60.067
PRIMER_RIGHT_3_TM=60.045
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=0.1811
PRIMER_LEFT_4_PENALTY=0.109391
PRIMER_RIGHT_4_PENALTY=0.071684
PRIMER_LEFT_4_SEQUENCE=CAAAGCTTAACAGTTGCTGTCG
PRIMER_RIGHT_4_SEQUENCE=AGACACAATTTACGTCTCCGGT
PRIMER_LEFT_4=1526,22
PRIMER_RIGHT_4=1803,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=59.928
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_03879 Ps_contig_mira-and-tgicl-ass_franssen_31350
SEQUENCE=AAGCAGTGATCAACGCAGAGTACGTCGGGGATCCAGTTATCAAACACACGCTAAATTTTGTCCGTTCACGAACACGAATCAACCGTCGAGTGGGAAATTGGAGTCTAGTTACTTGGTGGCAACTATTTACACTTTTCTTCTCTACAAAGAAGAAGAAGATAGAAACTTAAACCCTACAAAAAACACACATAATAACAACTTTTCACGGCGACGGCGACGGCGACGGCGACACCATAATCCGATGGCTTTGACAATCTCAAACATACTGCACTGCCCTAAATTGAATTCCTCTCGCAACCATCTCCGTCCCCAATTTTCATCCTCTCTCAGGTTTTCCAAGAGAACACTATGGAATAAACGAACGATTATATGTGCTTCTGCTTCTGCAGCAGGAAGTTCTAATCCTAATAGCGACATAAATCCTTATGAGGTTCTAGGTGTAAGCCCTATTGACAAGTTTGACAATATCAAAGCAGCATATACCAAAAAAAAGAAGGAGGCTGAGAACAATGGTGACGAAGAAACTGCTTCTAGACTAGAAAAGGCATATGACAAAGTTATGATGTCTCAATTGAGTAATCGGAAAAAGGGTCTGACTTTTGGATCATTTAAGGTTTCAAAGGACATAAAGTATGCCGATAAGCAGCCAATTATACCTTGGGGACCAAGGTTTGCTAAATCAAGTGAAAATGATATGCGCATCAACTTGGCATTATCTGCTGCTTTTACAGCTTGGATCGTGGTCACTCGCAGTGCTGAATATAAACCTTTGCAGTTTTTAGCTTTTGCTTTTGTTTATAGGCTTTTTGAGAAGTTAAAATCCTTTGAATCTCCTAAAAGCTCCACAATTACTGAAGATGGTGAAGATCCAGGAGAAGGACTGCGCATGGGAAAGAGACTGCTCCGGTCACTGGCCTTGGTTTTTGGATGTGTAGCTGTTTCATCCGTGGCATTCACTGTGGGTTTAAACATAATTGAGTCTGCAAGTGGTTCTATTCCATCTATTCTTTTTAACAGTCAGGAGTTGATAATCACCGCATCATCAGCAGTCATGCTTTATATTTTGGGATCTTTTTATCGATAGTGGACCTTTCTTGAAGACAAAGAAACCAGTCAATCTGGTGATGATCGAATACTGTGTTACTAATCTTCTTTTGATTTTTCATTTTCTATTACGAATCGAGTTATATCGTTGTTTCACTCAAATTTTGTCAAAAGAGAAATAGTTGTACTTCTAAAGTAGGAGAGAGTGCCAATTTAAAGTTTTGTTCTATTTTCTTTCG
TARGET=147,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2675
PRIMER_LEFT_PENALTY=0.081185
PRIMER_RIGHT_PENALTY=0.186309
PRIMER_LEFT_SEQUENCE=AAGCAGTGATCAACGCAGAGTA
PRIMER_RIGHT_SEQUENCE=TTAGGGCAGTGCAGTATGTTTG
PRIMER_LEFT=0,22
PRIMER_RIGHT=278,22
PRIMER_LEFT_TM=60.081
PRIMER_RIGHT_TM=60.186
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.4124
PRIMER_LEFT_1_PENALTY=0.081185
PRIMER_RIGHT_1_PENALTY=0.331254
PRIMER_LEFT_1_SEQUENCE=AAGCAGTGATCAACGCAGAGTA
PRIMER_RIGHT_1_SEQUENCE=CAATTTAGGGCAGTGCAGTATG
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=282,22
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=59.669
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.4835
PRIMER_LEFT_2_PENALTY=0.081185
PRIMER_RIGHT_2_PENALTY=0.402305
PRIMER_LEFT_2_SEQUENCE=AAGCAGTGATCAACGCAGAGTA
PRIMER_RIGHT_2_SEQUENCE=GTCGCCGTGAAAAGTTGTTATT
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.402
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.5096
PRIMER_LEFT_3_PENALTY=0.081185
PRIMER_RIGHT_3_PENALTY=0.428401
PRIMER_LEFT_3_SEQUENCE=AAGCAGTGATCAACGCAGAGTA
PRIMER_RIGHT_3_SEQUENCE=GAGAGGAATTCAATTTAGGGCA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=292,22
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=59.572
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.5706
PRIMER_LEFT_4_PENALTY=0.081185
PRIMER_RIGHT_4_PENALTY=0.489375
PRIMER_LEFT_4_SEQUENCE=AAGCAGTGATCAACGCAGAGTA
PRIMER_RIGHT_4_SEQUENCE=AATTCAATTTAGGGCAGTGCAG
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=286,22
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=60.489
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_03880 Ps_contig_mira-and-tgicl-ass_franssen_31351
SEQUENCE=TAGTTTTGTCGGGGGCCCCCACTTAACTGCCATGATCAAGTGAATGAAGCTGAATTAAAATAACTCTCTCTCCCTCTCTCCGACGACGACGAATTATGATAATGGTAGTGATTAATCTGATAAAGGATTTGTGGCGGGGTCTCCATTTCGTTCTATCGATTCAATTCTGGAGAATGGCGTTGCTATGGACACTTTCTGTTCTAGTTTCTCACTTCCAATTGCTTAAGGATTCGCTATTTTCTCACAAAATTGTATATCCAAGGTGTTCATCTTCCACGTTCCCTAATACTCCCGTTTGCGTCATCACCGGTGCAACATCTGGTTTGGGATTTTCTACTGCATGCAAGATTTCCAAGGAAGGATTTGTTGTCGTCATCGTTGGAAGATCAGAGCAGTTGTTATCAGAGACCATAAGAAAGATCAAAGATTTGAATGAGGATGCCCAACTCAAAGCTTTTCAGGCTGACCTTTCATCGGTTGAGTCCATTATAAAGTTCAAAGATTCTCTGCGGCAGTGGCTTCTGGACTCCAATTTGCACTGCTCGGTGCAAATACTGATAAACAATGCTGGAATACTTGCTACATCTCCTAGAGTCACGGCTGAGGGATACGATCAGATGATCGGCACAAATTATGTCGGCGCATTTGTTTTGACCAAGCTTCTATTACCACTTCTTGAAAACAGTTCCGTGTCTTCCAAAATTGTGAATGTAACATCCTTTACTCACCGAGCTGTTACTAATCTGCAGGTTGATGAGGGAATTGTATCTGGAAAGAAATTTTTAAGTTCAAAGCATTATCCATATGCTCAGGTCTATGAGTACTCGAAACTATGCGTACTTCTCTTCTCATTTGAGCTCCACCGACAGCTTACCCAAATGGGTAAATCTCATCAGATATTTGTCAATGTTGCAGATCCTGGAATTGTAAGAACTAACATCATGCGAGAAGTTCCAGCATGCCTGTCTTGGTTTGCATACTTTGTACTGAAACGCCTACGCCTATTGCAATCCCCTGACCGGGGGAAAGATTCGATTATTGATGCTGCTTTTGCTCCACCTGGAACATCAGGAGCTTACTTTTTTGGGGGGAAAGGTAGAACTATAAACCCTTCAGCACTTTCACGAAACGCCAAATTAGCACAACGAGCTTTGGGAAACTACATGCAATATGC
TARGET=81,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0888
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.088755
PRIMER_LEFT_SEQUENCE=AACTGCCATGATCAAGTGAATG
PRIMER_RIGHT_SEQUENCE=CGAATCCTTAAGCAATTGGAAG
PRIMER_LEFT=24,22
PRIMER_RIGHT=232,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.089
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.1647
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=AACTGCCATGATCAAGTGAATG
PRIMER_RIGHT_1_SEQUENCE=ATTAGGGAACGTGGAAGATGAA
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=286,22
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.2333
PRIMER_LEFT_2_PENALTY=0.000037
PRIMER_RIGHT_2_PENALTY=0.233224
PRIMER_LEFT_2_SEQUENCE=AACTGCCATGATCAAGTGAATG
PRIMER_RIGHT_2_SEQUENCE=GTGGAAGATGAACACCTTGGAT
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=276,22
PRIMER_LEFT_2_TM=60.000
PRIMER_RIGHT_2_TM=60.233
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.3524
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.352395
PRIMER_LEFT_3_SEQUENCE=AACTGCCATGATCAAGTGAATG
PRIMER_RIGHT_3_SEQUENCE=TTAGGGAACGTGGAAGATGAAC
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=285,22
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.352
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.3830
PRIMER_LEFT_4_PENALTY=0.000037
PRIMER_RIGHT_4_PENALTY=0.382951
PRIMER_LEFT_4_SEQUENCE=AACTGCCATGATCAAGTGAATG
PRIMER_RIGHT_4_SEQUENCE=ATGACGCAAACGGGAGTATTAG
PRIMER_LEFT_4=24,22
PRIMER_RIGHT_4=303,22
PRIMER_LEFT_4_TM=60.000
PRIMER_RIGHT_4_TM=60.383
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_03881 Ps_contig_mira-and-tgicl-ass_franssen_31352
SEQUENCE=AAACGAACACATCTTTGGAGGTANGCGTRCGTCTCGTTGGTGGTTAAGTCACTGTCCTTTCGCACCCTCGACGAGTCCGCCATCTTCCAAACAAAAAACACTTCACTCTTTCTCTTCTCACCAAACAAAATTCTTTATTACATTTATAACAACCTCATTCATCTAACTCATCTATCTCAATCAAATTTATCAATTAATGGACGCTTCTACCCTCTCCCGTATTGGCCTCGCCGGCCTCGCCGTCATGGGCCAAAACCTAGCCCTAAACATCGCCGAGAAAGGCTTCCCTATCTCCGTCTACAACCGCACCACCTCCAAAGTCGATGAAACCGTAGATCGAGCCCACCGTGAAGGCTCCCTCCCTCTCACCGGTCAATACAGCCCCCGCGAGTTTGTTCTCTCTCTCCAACGGCCTAGATCGGTTATCATCCTCGTCAAAGCCGGTGCTCCTGTTGATCAAACAATCGCCGCACTGTCGGACCATATGGAGCCTGGTGATACTATCATCGACGGTGGTAACGAGTGGTACGAAAATACTGAACGGAGGATTCAGGAGGTTTCAGAGAAAGGTATTCTTTATCTCGGTATGGGGGTTTCTGGAGGCGAAGAAGGTGCGAGGAACGGTCCGTCGCTTATGCCGGGTGGATCTTACCAGGCTTATAGTAATATTCAGGATATTCTTGTGAAGGTTGCTGCTCAGGTTGAAGATGGACCTTGTGTTACTTATATCGGTGAAGGAGGGAGTGGGAATTTTGTTAAGATGGTTCATAATGGGATTGAGTATGGTGATATGCAGCTTATTTCTGAAGCTTATGATGTTTTGAAGCATGTTGGTGGTTTGTCGAATTCGGAGCTTGCTGATATTTTTGCTGAGTGGAATAGCGGTGAGCTTGAGAGTTTTCTTATTGAGATTACTGCTGATATTTTCAAGGTGAAGGATGAGGGTGGTGATGGGTATTTAGTGGATAAGATTTTGGATAAAACTGGTATGAAAGGTACTGGGAAATGGACGGTTCAACAGGCGGCGGAGTTATCGGTGGCGGCTCCGACTATTGCGGCGTCGTTGGATGGTAGGTTTTTGAGTGGATTGAAGGAGGAGAGGGAGAATGCTGCTGCGGTTTTGAAGGAAGCGGGGTTGAGTGAGGAAGTAGGGTTCGTGAGAAGTGGGATTGATAAGAAAAAGTTGATTGATGATGTGAGACAAGCTTTGTATGCTTCCAAGATTTGTAGCTATGCGCAAGGGATGAATTTGTTGAGAGCAAAGAGTAATGAGCAAGGTTGGAACTTGAATTTCGGTGAGTTAGCTAGGATTTGGAAAGGTGGGTGTATTATACGGGCAGTGTTTTTGGATAGGATTAAGAAGGCTTATCAGAGGAACCCTAATTTGGCGAGTTTGATAGTGGATCCCGAATTCGCTAAGGAAATGGTGCAGAGACAAGGTGCGTGGAGAAGGGTTGTTGGATTGGCTATATCGGCCGGAATTAGTACTCCCGGAATGTGTGCTAGTCTTTCGTACTTTGATACATATAGGAGAGCAAGGCTTCCTGCTAACCTTGTTCAGGCTCAGAGGGACTTGTTTGGAGCTCATACCTATGAGCGTCTCGATCCGGCCCCGGCGTCGTTTCATACCGAGTGGACGAAACTTGCTCGTACAAGAGGTTGGGTCGCGTAGGCTTAACTTTTACTTTAA
TARGET=397,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03882 Ps_contig_mira-and-tgicl-ass_franssen_31354
SEQUENCE=ACNCTGCCTAGATTCGGTAGCCATCGCCAAACCAAATGCACTGAACTGTAAACTCTAACCATGCTAACATGTCTATATAAATGAAAAATAATCACACACATTCAATTCAACTCGCTTTCCTTCTTCAATGGCTTCTCTCATCACCTTCCTCAAGCGAACCTCTTCATCATCATCATCATCTTTTGCAGTTGCATGCCACCATTCTATCATCATGAGAAGATCCTTAACGACAACAACAGCAGCAGCAGCTACTGTTGATTCTTCATCCTTTGCTCAGAGAATCAGAGATTTGCCTAAAGATCTTCCTGGTACCAACATCAAAAAACATGTTTCTCAGTTAATTGGGAGGACTCCACTGGTTTATCTTAACAAAGTTACTGAGGGATGTGGAGCTTATATTGCTGTTAAGCAAGAGATGATGCAACCTACTGCAAGCATCAAAGACAGACCGGCATTTGCTATGATGGAAGATGCTGAGAAAAAAAATCTGATATCTCCTGGAAAGACTGTTCTGATAGAGCCGACATCGGGAAACATGGGGATCAGTTTGGCATTTATGGCTGCCATGAAGGGATATAAGATGGTTTTGACTATGCCTTCTTACACAAGCTTGGAAAGAAGGGTTTGTATGAGAGCATTTGGAGCTGAGTTGGTCCTCACTGATCCAACTAAAGGAATGGGTGGAACAGTTAAAAAGGCATATGACCTTTTGGAATCCACACCAGATGCTTTCATGCTGCAACAATTTTCCAATCCAGCAAATACTCGGGTGCATTTTGAAACAACAGGTCCTGAGATATGGGAGGATACAAATGGACAAGTGGACATATTTGTTATGGGAATAGGTAGCGGCGGCACAGTTTCCGGGGTCGGACAATATCTTAAATCCCGAAACCCCAACGTTAAGATATATGGAGTGGAGCCATCTGAAAGCAATGTGCTCAATGGTGGTAAACCAGGTCCTCATCATATAACTGGAAATGGTGTTGGATTCAAACCAGATATATTGGACATGGATGTAATGGACAAAGTTCTTGAAGTTAGCAGTGAAGATGCAGTTAACATGGCTAGGACATTGGCCTTGAAAGAAGGACTCATGGTTGGCATATCGTCCGGAGCTAACACAGTTGCAGCACTTAGATTGGCTAGTCTTCCTGAGAATAAAGGCAAACTTATTGTGACTGTTCATCCAAGTTTCGGGGAACGATACTTGTCATCTGTCTTGTACCAAGACCTTAGGACAGAAGCTGAAAACATGCAGCCTGTGTCCGTCGATTAATTCCTTCCATGGGCTCCGATTCATCTAGAAAGATTTGTTGTGGAGTTACATGGATCAATTGGTTGTTGATGTTGTAGCAAAAAAATTCATGTTCAGCTTACAATTCTGCCATAGTAATAAATGTCAGCCAAAGCTATGGTTTTTACATATCTTATAAACTTCTTGTCTTAATGCTTTGTAATGTTTTGTTTGATATGATGAACATTGTAATATGTTCTAAAATGAAAGTATGAAATTGTAATATGAGGTACATTGTGTTTTCTTTTGGCACATGATGTGTGCATTCAGTTATCTAAARCNGACCAATCCAA
TARGET=164,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03883 Ps_contig_mira-and-tgicl-ass_franssen_31354
SEQUENCE=ACNCTGCCTAGATTCGGTAGCCATCGCCAAACCAAATGCACTGAACTGTAAACTCTAACCATGCTAACATGTCTATATAAATGAAAAATAATCACACACATTCAATTCAACTCGCTTTCCTTCTTCAATGGCTTCTCTCATCACCTTCCTCAAGCGAACCTCTTCATCATCATCATCATCTTTTGCAGTTGCATGCCACCATTCTATCATCATGAGAAGATCCTTAACGACAACAACAGCAGCAGCAGCTACTGTTGATTCTTCATCCTTTGCTCAGAGAATCAGAGATTTGCCTAAAGATCTTCCTGGTACCAACATCAAAAAACATGTTTCTCAGTTAATTGGGAGGACTCCACTGGTTTATCTTAACAAAGTTACTGAGGGATGTGGAGCTTATATTGCTGTTAAGCAAGAGATGATGCAACCTACTGCAAGCATCAAAGACAGACCGGCATTTGCTATGATGGAAGATGCTGAGAAAAAAAATCTGATATCTCCTGGAAAGACTGTTCTGATAGAGCCGACATCGGGAAACATGGGGATCAGTTTGGCATTTATGGCTGCCATGAAGGGATATAAGATGGTTTTGACTATGCCTTCTTACACAAGCTTGGAAAGAAGGGTTTGTATGAGAGCATTTGGAGCTGAGTTGGTCCTCACTGATCCAACTAAAGGAATGGGTGGAACAGTTAAAAAGGCATATGACCTTTTGGAATCCACACCAGATGCTTTCATGCTGCAACAATTTTCCAATCCAGCAAATACTCGGGTGCATTTTGAAACAACAGGTCCTGAGATATGGGAGGATACAAATGGACAAGTGGACATATTTGTTATGGGAATAGGTAGCGGCGGCACAGTTTCCGGGGTCGGACAATATCTTAAATCCCGAAACCCCAACGTTAAGATATATGGAGTGGAGCCATCTGAAAGCAATGTGCTCAATGGTGGTAAACCAGGTCCTCATCATATAACTGGAAATGGTGTTGGATTCAAACCAGATATATTGGACATGGATGTAATGGACAAAGTTCTTGAAGTTAGCAGTGAAGATGCAGTTAACATGGCTAGGACATTGGCCTTGAAAGAAGGACTCATGGTTGGCATATCGTCCGGAGCTAACACAGTTGCAGCACTTAGATTGGCTAGTCTTCCTGAGAATAAAGGCAAACTTATTGTGACTGTTCATCCAAGTTTCGGGGAACGATACTTGTCATCTGTCTTGTACCAAGACCTTAGGACAGAAGCTGAAAACATGCAGCCTGTGTCCGTCGATTAATTCCTTCCATGGGCTCCGATTCATCTAGAAAGATTTGTTGTGGAGTTACATGGATCAATTGGTTGTTGATGTTGTAGCAAAAAAATTCATGTTCAGCTTACAATTCTGCCATAGTAATAAATGTCAGCCAAAGCTATGGTTTTTACATATCTTATAAACTTCTTGTCTTAATGCTTTGTAATGTTTTGTTTGATATGATGAACATTGTAATATGTTCTAAAATGAAAGTATGAAATTGTAATATGAGGTACATTGTGTTTTCTTTTGGCACATGATGTGTGCATTCAGTTATCTAAARCNGACCAATCCAA
TARGET=237,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03884 Ps_contig_mira-and-tgicl-ass_franssen_31357
SEQUENCE=GACCCAAATAATCCTGTGTTAGCTAAAATTTCATAAGAAATCATTTTACACAGCAAAAACATCCAAAAGGGTGTATAACAAACATCATCCTCTGAGACATAAAARGAGAATATGACAAATACACACCACCTAAGATCAGAGCAGATGTACGAAAACACAAAATACCGAAACCATGATTCAATAAAACAAAATCAAAAGAGTCAAGACAAATCCTAGCAAGCCATTCATACTTATACACTTGTTAACCAACACTATTAAACACGGCCAGTACCTTGTTTAGCCTTTGACTCATAAAAGAGCCTCACAGTGGAGTAAATGGCCGAAGGTTCCGCCATTGCGCCATTGCCATAATCCCCACAAGCCAACCTCTTTGTAACCGGTGGAATGAGAGAAATACCAAGCTCGTCAATGGAAATGAGATGCTTCTCCGTGAAAGGATTGTTCCACATAAAAGTGTTCATGGCCGGTGCAACAAAGAACGGCTTACTGTAGTCCCAGGCCCTCGCGATACACGTCAGTAAATTGTCACACAACCCTCCAGCAATCTTGGCAAGAGTGTTTGCGGATAATGGAGCAATGACCATGATATCAGCCCATTTGCGAAGCTCAATGTGAAGCACGCTATCGCCTAGTTTCTTCCAACTGGACCATTCGTCGTTATCGGTATACAAAATCACATCCTTGGGAATCGCCGTTCTGTCAATAAAATGCAAAGATGGATTTGTGGCAACTGCTCTTACTTCAGCCCATTCGCAGAAACAGTGACAAAGATTTGCAAATTTAACAGCAGCAACACTCCCGCTAGTCGCAAGTAAAATCCGAGGCTTCCTGGGTGAAGTAACCACTGACATATTCTCTCCCACTGAGCTCACAGGCTCTGAGCAGGCCATAATCAACCAGAAATTTCAAACAAAGAGAGAGAGAGTCACTCACAATAAAGGTAAAACTTTAATGCGTGT
TARGET=914,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03885 Ps_contig_mira-and-tgicl-ass_franssen_31360
SEQUENCE=ACTACGGGGAGAGAAAGTTCAGGGATATGGTTATTATGGTTGGCGGTGGTTTGAAGGCGGCGATGTTAATGTCGTCGTCGTTGAGTCACGTGCGTCCTCTAACATGCGCTTCGTCGTCGCGATTAGTGTCGCATCCACACGACCTTGTGAAATGGGTAAAAAGAGAAGGTGGTTTCGTTCACCGTGCGGTGAAGATAGCGCAACCCGATTCCGGTGGTTTAGGGTTAGGGTTAGGGTTAGTGGCTAAAGAAGAAATTCCGATTGGTACTGACCTAATTGTTCTTCCTCAGCATCTTCCTTTGCATTTCACGTCTTTTCATCAGGATCAGAAGAATGATGAGAACTCTGTTCTCCTTCAATTGAGCTCACATGTTCCTGATGAATTATGGGCAATGAAACTGGGTTTGAAGCTTCTCCATGAAAGGGCAAAACATGGATCTTTTTGGTGGCCATATATCAGTAATCTCCCAGAAACTTACACTCTTCCGATCTTCTTTCCTGGGGAAGATATCAAGAATTTGCAGTATGCTCCGATTCTTCACCAGGTAAACAAAAGATGCCGATTTCTTCTTGATTTTGAGAAACAAGTCAAGCGTGCGCTAGTTGGTGTTACACCAGATAAGCATCCTTTTGGTGGTCAAGAAGTAGATGCTTCGTCTCTGGGATGGGCAATGTCAGCAGTTTCATCTAGAGCATTCAAGTTACATGGTAACAAGAATTCAAATGGGATAAACTTTGATATACCGATGATGCTCCCTCTAATTGACATGTGTAATCATAGTTTCAATCCAAATGCTAGAATTGTTCAGGAGCAAGAAACTGGTAGCACAGACATGCGTGTGAAGGTTGTGGCTGAGAAAGTCATCAGGGAAGATGATCCTTTATTACTCTGCTACGGATGTTTAAACAATGATCTTTTCCTTCTTGATTACGGATTCGTGATACAATCAAACCCTTATGACTGTATCGAGCTCAGATATGATGGTGCTCTTTTGGAGGCGGCAAGCACGGCAGCTGGAGTTTCTTCACCAAATTTCTCCACACCTGCTCCATGGCAGGAACTTATTTTATCACAGCTGAATCTAGCTGGAGAAGCTCCTGATCTCAAGGTGAGCATAGGTGGTCAAGAAATAATCGAAGGTCGTTTATTGGCCGCACTGAGAGTCCTCCTTGCAAGTGATATGGAAACTGTACAAAAGTATGACCTCAGCACACTCAAGTCTGTAGATGCTGAGGCTCCACTTGGTGTTGCGATTGACATTGCTGTATTTCGTACTTTGATCGCCCTATGTGTCGTTGCATTGGGACACTTTCCCACCAAATTAATGGATGATGAATCCTTATTGAAGCAGGGTGCTTCTGGTTCAACAGAGTTAGCCATTCAGTTCAGAATCCAAAAGAAATCTATAATTATAGATGTGATGAAAAACCTTGCTAGGAAGGTAAAGGTTCTTTCATCAAATGAGACAGTTACTGCTGAAGGCTAAGATTCAACACTTTCTGGCATGAATCTCATCTCGAATCTTCTTTTGTCTTGTCTTGTCATGTGATTTTTATTCTTTTAAGATTCTAACTGTATTATTGTTTTTTTGTTCGAGATGGTCAACTATGAAGTTCTAGATTCCATGTTTACCTTAGTAATATCGATATTTATCTACTATTGAATTAGA
TARGET=1530,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8057
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=1.771597
PRIMER_LEFT_SEQUENCE=GAATCCTTATTGAAGCAGGGTG
PRIMER_RIGHT_SEQUENCE=TCATAGTTGACCATCTCGAACA
PRIMER_LEFT=1334,22
PRIMER_RIGHT=1611,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=58.228
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=278
PRIMER_PAIR_PENALTY_1=1.8894
PRIMER_LEFT_1_PENALTY=0.117817
PRIMER_RIGHT_1_PENALTY=1.771597
PRIMER_LEFT_1_SEQUENCE=GATTCAACACTTTCTGGCATGA
PRIMER_RIGHT_1_SEQUENCE=TCATAGTTGACCATCTCGAACA
PRIMER_LEFT_1=1487,22
PRIMER_RIGHT_1=1611,22
PRIMER_LEFT_1_TM=60.118
PRIMER_RIGHT_1_TM=58.228
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=1.8894
PRIMER_LEFT_2_PENALTY=0.117817
PRIMER_RIGHT_2_PENALTY=1.771597
PRIMER_LEFT_2_SEQUENCE=TCAACACTTTCTGGCATGAATC
PRIMER_RIGHT_2_SEQUENCE=TCATAGTTGACCATCTCGAACA
PRIMER_LEFT_2=1490,22
PRIMER_RIGHT_2=1611,22
PRIMER_LEFT_2_TM=60.118
PRIMER_RIGHT_2_TM=58.228
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=2.0640
PRIMER_LEFT_3_PENALTY=0.292414
PRIMER_RIGHT_3_PENALTY=1.771597
PRIMER_LEFT_3_SEQUENCE=TAGCCATTCAGTTCAGAATCCA
PRIMER_RIGHT_3_SEQUENCE=TCATAGTTGACCATCTCGAACA
PRIMER_LEFT_3=1374,22
PRIMER_RIGHT_3=1611,22
PRIMER_LEFT_3_TM=59.708
PRIMER_RIGHT_3_TM=58.228
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=2.0865
PRIMER_LEFT_4_PENALTY=0.314939
PRIMER_RIGHT_4_PENALTY=1.771597
PRIMER_LEFT_4_SEQUENCE=TGAGACAGTTACTGCTGAAGGC
PRIMER_RIGHT_4_SEQUENCE=TCATAGTTGACCATCTCGAACA
PRIMER_LEFT_4=1462,22
PRIMER_RIGHT_4=1611,22
PRIMER_LEFT_4_TM=59.685
PRIMER_RIGHT_4_TM=58.228
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_03886 Ps_contig_mira-and-tgicl-ass_franssen_31365
SEQUENCE=ACCACTATTTGATCCTCCTAGGCATGATAGTGCTGAAACCATCACAAGAGCTCTGAACCTTGGTGTGAATGTTAAGATGATTACCGGTGATCAGCTTGCCATTGCCAAGGAAACTGGCCGAAGGCTTGGGATGGGAACAAACATGTATCCATCTTCGTCATTACTCGGTCAAAGCAAGGATGCTGCTGTTGCAGCTCTTCCAGTTGACGAGTTGATTGAGAAGGCTGATGGATTTGCTGGAGTATTTCCTGAACACAAATATGAAATCGTAAAGAGGCTGCAAGAGAGGAAACATATATGTGGAATGACAGGTGATGGTGTCAACGATGCTCCTGCGCTAAAGAGAGCAGATATTGGAATTGCTGTTGCTGACGCTACAGATGCTGCTAGAGGTGCTTCTGATATTGTCCTTACTGAACCCGGTCTAAGTGTTATTATCAGTGCAGTGCTCACCAGCAGGGCAATTTTCCAAAGGATGAAGAATTATACTATCTATGCTGTGTCAATCACCATTCGTATTGTGTTTGGTTTCATGTTCATTGCGTTGATCTGGAAGTTTGATTTTGCACCCTTCATGGTGTTGATTATCGCCATACTGAATGATGGTACCATTATGACAATATCCAAGGATAGAGTGAAACCATCTCCACTACCAGACAGTTGGAAACTGAAAGAGATATTTGCTACCGGTGTTGTGCTAGGTAGCTACATGGCATTAATGACAGTAGTATTTTTCTGGCTCATGAAGGATACCGACTTCTTCTCGGACAAGTTTGGTGTGAGGTCTCTCAGAAAAAGCCCTGATGAAATGATGGCAGCCCTCTACCTACAAGTCAGTATTATAAGCCAGGCCCTAATTTTTGTCACCAGGTCCCGTAGCTGGTCCTTTTTGGAAAGACCTGGTCTTCTCCTATTGGGTGCTTTTATGATTGCTCAGCTGGTGGCAACTTTTATAGCAGTATATGCTAATTGGGGCTTTGCAAGAATTAAGGGAATGGGATGGGGTTGGGCTGGTGTAATCTGGGTGTACAGTCTAGTGACATATTTCCCTCTTGATATACTCAAATTCGTAATCCGCTATGTTCTAAGTGGCAAAGCTTGGGATAATCTTTTGGAGAACAAGACTGCTTTCACAACAAAGAAAGACTACGGCAAAGAAGAGAGAGAAGCACAGTGGGCAACTGCTCAGAGGACTCTCCATGGTCTTCAGTCACCAGAAACAACCAACCTTTTCAATGACAAGAATAGCTACAGGGAGCTTTCTGAGATTGCTGAGCAAGCCAAGAGACGCGCTGAGGTTGCACGGCTTAGGGAGCTTCATACTCTAAAGGGTCACGTCGAATCAGTTGTGAAGCTTAAGGGACTTGACATTGACACTATGCAACAACATTACACTGTTTAAGGAGCTTAGACAACCTTCAAGATGAACAGGGTTATGTGCTTGTTCAAGATGCAAAAACAAGAAATACAGTGAAGACTTGAGTTAATCTTATGTCTTTAGATTAGGTTGAAGGGTCCTTTTGTGAAAGTTTGAATGGTTACTCCTATACGCCAGTAGCTAGAGGAGATACTATTCTCTTAAGTACCAAATAACATGGTTGATAGGATTTACTTGCGTCTTAGTTTTGGAAATGTTAGTGATATTAAGGTCACAAAAAGCTCTATATTTTTTAGCTGAACAAAACTCAATAGCTCGACCTTGAGTAGTGATGTTCAATAATGAATGAATGCAATTCTTTTGGTCTCTTTTACCTGCCTAACT
TARGET=1719,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7083
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.696189
PRIMER_LEFT_SEQUENCE=TGAAGGGTCCTTTTGTGAAAGT
PRIMER_RIGHT_SEQUENCE=AGGCAGGTAAAAGAGACCAAAA
PRIMER_LEFT=1508,22
PRIMER_RIGHT=1757,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=59.304
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=0.7083
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.696189
PRIMER_LEFT_1_SEQUENCE=TGAAGGGTCCTTTTGTGAAAGT
PRIMER_RIGHT_1_SEQUENCE=GGCAGGTAAAAGAGACCAAAAG
PRIMER_LEFT_1=1508,22
PRIMER_RIGHT_1=1756,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=59.304
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=1.0608
PRIMER_LEFT_2_PENALTY=0.364634
PRIMER_RIGHT_2_PENALTY=0.696189
PRIMER_LEFT_2_SEQUENCE=GTTGAAGGGTCCTTTTGTGAAA
PRIMER_RIGHT_2_SEQUENCE=AGGCAGGTAAAAGAGACCAAAA
PRIMER_LEFT_2=1506,22
PRIMER_RIGHT_2=1757,22
PRIMER_LEFT_2_TM=60.365
PRIMER_RIGHT_2_TM=59.304
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=1.0608
PRIMER_LEFT_3_PENALTY=0.364634
PRIMER_RIGHT_3_PENALTY=0.696189
PRIMER_LEFT_3_SEQUENCE=GTTGAAGGGTCCTTTTGTGAAA
PRIMER_RIGHT_3_SEQUENCE=GGCAGGTAAAAGAGACCAAAAG
PRIMER_LEFT_3=1506,22
PRIMER_RIGHT_3=1756,22
PRIMER_LEFT_3_TM=60.365
PRIMER_RIGHT_3_TM=59.304
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=1.1579
PRIMER_LEFT_4_PENALTY=0.461713
PRIMER_RIGHT_4_PENALTY=0.696189
PRIMER_LEFT_4_SEQUENCE=AGGGTCCTTTTGTGAAAGTTTG
PRIMER_RIGHT_4_SEQUENCE=GGCAGGTAAAAGAGACCAAAAG
PRIMER_LEFT_4=1511,22
PRIMER_RIGHT_4=1756,22
PRIMER_LEFT_4_TM=59.538
PRIMER_RIGHT_4_TM=59.304
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_03887 Ps_contig_mira-and-tgicl-ass_franssen_31369
SEQUENCE=ATATTTAAAACCTCAAAAAAAAAAAAAAAAAATTACGCATAAACCATCACTACTCGGCATTCAGTTACTTTTCCCAAGTCGCGTTTTGTGTGATAATGGCGCCCGATGCTGCCGCCGCCGCCGCTGGTGGAAAGAAGAGAAAACGCTATCTTCCTCACAACAAAGCAGTGAAGAAGGGTTCGTATCCATTGCATCCTGGTGTGCAAGGTTTTTTCATCACTTGCGATGGAGGAAGAGAACATCAAGCCTCACGCGAAGCTCTCAATATTCTCGATTCCTTTTATGAAGATCTAGTTGACGATGGGGAACATCCAACTGTGAAAGGGCTGTCAAATAGACCTGTAAATACAAAAATTACATTTGCAGATTCCGACTCTTCTAGTAGTGATGACGAAGAGGAAGAGGATGAGGATGGGAAGGAGGAGGTGCAGGTGAAGGTGCAGGAGGGTGAGGAAAAGGAGGACAAAAAGCCTAAACTGGATGTTAGCAATGGGGACAATACTGACCATGAGAATGGAACGGGTGAAAAATCAGATCCTCATAAAATTGATGATCTTCATGCTCAGGCTGGGGATAAAGCTGATGGCGATAAAGAAGGTGTTGACATTCCTAAGACTATAGAAAAGACAGCTGATGAGCCACCAGCTGTGAAACAGTGCTGCGCAACAAATGTGCCAACAAGTGATTTGGGTGAAAAGAAAGTGGAAGAGAAATCCATTGACAAGTTAATTGAAGATGAACTTGTAGAACTGAGAGACAAGAATAAGAAGCGCTTTGCCAAGCTTGAATCAGGTTGTAATGGGGTCGTATTTATTCAAATGAGGAAGAAAGATGGAGACAAAAGCCCCAAGGATATCGTTACTCGGATAGTGACATCAGCAGCATTGACTAAGAAACATATGTCAAGGTTTATCTTGAGAATTTTGCCAATTGAAGTCTCATGTTATGCTTCAAAGGAGGAAATTTCAAAAGCAATCCAGCCTCTTGTGGAACAGAACTTTCCTGCGGAAACTGAAAATCCACACAAGTTTGCTGTGCTGTATGAAGCCCGGGCAAATACTGGTGTTGACAGGATGGAAATAATCAATGCCGTGGCAAAGTCTATTCCTGCACCTCATAAAGTTGATCTCACTAATCCTGATAAAACCATAATAGTTGAAATTGCCAGGACTGTATGCTTGATTGGGGTCATCGAGAAGTACAAGGAACTCTCCAAGTACAATATTAGGCAATTAACATCACGATAAGTACGAGTCTTCCAGACAGTCTGTATGCTTGTGGTTGTGGCAAACTTAATACCTTTAGTATTTATACTTGCAGCAAATCGTAGTTTTAGCAAGTCCTTTAAATGTAATTTAGTTCTCACAAACCAAAGACAATATTATGCTTGAATTCCGGTTAGAATATTAGTTTCAAATCATTTAGAATATCTGTTGTATTCATGCTTTGATGTGATTTTCTTAACATAAATGACTTTTGAAACGCGTTTATAAACATAATGTTATATTGGTCCTCATCGTGGCCAGCCATTGCTCTC
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0202
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=CATAAACCATCACTACTCGGCA
PRIMER_RIGHT_SEQUENCE=TGGATACGAACCCTTCTTCACT
PRIMER_LEFT=37,22
PRIMER_RIGHT=187,22
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=11.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=0.0202
PRIMER_LEFT_1_PENALTY=0.017846
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=CATAAACCATCACTACTCGGCA
PRIMER_RIGHT_1_SEQUENCE=GGATACGAACCCTTCTTCACTG
PRIMER_LEFT_1=37,22
PRIMER_RIGHT_1=186,22
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=11.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=150
PRIMER_PAIR_PENALTY_2=0.0316
PRIMER_LEFT_2_PENALTY=0.017846
PRIMER_RIGHT_2_PENALTY=0.013714
PRIMER_LEFT_2_SEQUENCE=CATAAACCATCACTACTCGGCA
PRIMER_RIGHT_2_SEQUENCE=GAGAATATTGAGAGCTTCGCGT
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=271,22
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.7000
PRIMER_RIGHT_2_END_STABILITY=11.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=235
PRIMER_PAIR_PENALTY_3=0.0562
PRIMER_LEFT_3_PENALTY=0.017846
PRIMER_RIGHT_3_PENALTY=0.038361
PRIMER_LEFT_3_SEQUENCE=CATAAACCATCACTACTCGGCA
PRIMER_RIGHT_3_SEQUENCE=CCCCATCGTCAACTAGATCTTC
PRIMER_LEFT_3=37,22
PRIMER_RIGHT_3=305,22
PRIMER_LEFT_3_TM=60.018
PRIMER_RIGHT_3_TM=59.962
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=0.0577
PRIMER_LEFT_4_PENALTY=0.017846
PRIMER_RIGHT_4_PENALTY=0.039879
PRIMER_LEFT_4_SEQUENCE=CATAAACCATCACTACTCGGCA
PRIMER_RIGHT_4_SEQUENCE=ATTTGACAGCCCTTTCACAGTT
PRIMER_LEFT_4=37,22
PRIMER_RIGHT_4=334,22
PRIMER_LEFT_4_TM=60.018
PRIMER_RIGHT_4_TM=60.040
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=11.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_03888 Ps_contig_mira-and-tgicl-ass_franssen_31374
SEQUENCE=CCTATTGAAAGTTAGGATTGCATTAATTCTGAGCTACCAAAAGGAAAACAACNCATAGGAAAAGATCTTATCATTAGACATTACAAGTAGCAACAAGAAGTATTACTGCCATTCACAACCTGAGTAGCTTGCACTAACTATAATCGCAGAATACATGACGAAAGAAAATAAAAAAGGCCTTATACATTAGTTCAGCAGAAGTAAAATAAAGATTCATATCTGCGACCAACTTACTAGTGTAATACAACAACTTAAAATAAATAACACATACAGAACTTTCATGAATTAATGTTATCTAAATCATATGGTAATGAGTGAGGTTGAATTTTCATCTTGAACTACCTCCTCCTCCTCCGCATCGGAACAATTCCATCCCAAGTTCACAGCCAAGTCTTCTACCCCGACTTTGATGACATCACTTCTCACACCAATATCAGTTGCATATCTACTCACGCAGTACATTCCTGCTGTCACCGTAGCGATACCGACAGGACTTGGCATTAACAATTTGAGAGGAGAAGAAAGCATAGCGGCCAACGGAGCACCGATAGCAGAAGAAGCTTGACCACTTCTTCCAACTCCTTGTTTCTCAACAATCAAAAGACCGGTTTTGCAGTACATTGCAAAATCCTCACAGTTGTTCTGAAAAACATCATAGTTACCGAAACCGTTTTGAAGGAGATACATTGCTCGATGGATAACGGTCTCCGGCGGGTCAGGTAACGCGGTAGTGCACGTGCCACCTCTTATTCTGGTGAGAAAAAGTGTTGGTGAAACTCCATATTCGAAACGGTAAAGAGAACCGTTTCGAAGGAAGCAGTCCAGGCAAGAAAGAACGACTCCACTGTTTGGTTGACGGAATCCACAATCAGGAAAGGTTGGACACGGGGTTGGTGGTGGATCGTTAAAGTTGGAAGATGTCTCGGTGATTGACTTGAAGTTTCGATCAGGTCTGAAATGTACTACCTTGCCTTACCCCCAAAACTAAAAACTTCCGTGATGAGAGTAAGTAGAAAACNGCTNTGTAAGTGAGAGAGTGGTCGTACCGGGTT
TARGET=342,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2637
PRIMER_LEFT_PENALTY=0.168114
PRIMER_RIGHT_PENALTY=0.095574
PRIMER_LEFT_SEQUENCE=TTACTGCCATTCACAACCTGAG
PRIMER_RIGHT_SEQUENCE=GTGAACTTGGGATGGAATTGTT
PRIMER_LEFT=102,22
PRIMER_RIGHT=383,22
PRIMER_LEFT_TM=60.168
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.2666
PRIMER_LEFT_1_PENALTY=0.264300
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=CGCAGAATACATGACGAAAGAA
PRIMER_RIGHT_1_SEQUENCE=GTCATCAAAGTCGGGGTAGAAG
PRIMER_LEFT_1=144,22
PRIMER_RIGHT_1=413,22
PRIMER_LEFT_1_TM=60.264
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.2666
PRIMER_LEFT_2_PENALTY=0.264300
PRIMER_RIGHT_2_PENALTY=0.002310
PRIMER_LEFT_2_SEQUENCE=CGCAGAATACATGACGAAAGAA
PRIMER_RIGHT_2_SEQUENCE=CATCAAAGTCGGGGTAGAAGAC
PRIMER_LEFT_2=144,22
PRIMER_RIGHT_2=411,22
PRIMER_LEFT_2_TM=60.264
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.2989
PRIMER_LEFT_3_PENALTY=0.203324
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=CTGCCATTCACAACCTGAGTAG
PRIMER_RIGHT_3_SEQUENCE=GTGAACTTGGGATGGAATTGTT
PRIMER_LEFT_3=105,22
PRIMER_RIGHT_3=383,22
PRIMER_LEFT_3_TM=59.797
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.3599
PRIMER_LEFT_4_PENALTY=0.264300
PRIMER_RIGHT_4_PENALTY=0.095574
PRIMER_LEFT_4_SEQUENCE=CGCAGAATACATGACGAAAGAA
PRIMER_RIGHT_4_SEQUENCE=GTGAACTTGGGATGGAATTGTT
PRIMER_LEFT_4=144,22
PRIMER_RIGHT_4=383,22
PRIMER_LEFT_4_TM=60.264
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_03889 Ps_contig_mira-and-tgicl-ass_franssen_31376
SEQUENCE=GTGGTATCAACGCAGAGTCGCGGGGAACACAGTTTCTTTGCAGCAGCATAACACAAGTATTTCGTTGCCGTATCCATTCACAAATCAAACACTAACAACATGGCAGAACGTGGTGGCGGAGACCGCGGTGGCTTCGGCAGCGGATTCGGCGGAAGAGGAGGACGTGGCGGCCGTGGTGACAGAGGCCGCGGTGGTCGCAGAAGAGCCGGTGGAAGACGTGAGGAAGAGGAAAAATGGGTTCCAGTGACCAAGCTAGGTCGTCTCGTCAAGGAAGGAAAGATCCGCAGCCTCGAACAGATCTACCTCCACTCTCTCCCAATCAAGGAGCACCAAATCATCGACACTCTCGTCGGTCCAACCCTCAAGGACGAAGTCATGAAAATCATGCCGGTTCAGAAACAAACCCGAGCCGGTCAGAGAACCCGGTTCAAGGCCTTTGTTGTTGTTGGTGACAATAACGGTCACGTTGGTCTCGGTGTTAAGTGCAGCAAAGAAGTTGCTACTGCAATTCGTGGTGCGATTATCTTGGCTAAGCTCTCTGTTATTCCTGTTAGGAGAGGTTATTGGGGTAACAAGATCGGAAAGCCCCACACTGTTCCTTGTAAGGTTACCGGAAAGTGTGGTTCGGTTACTGTGCGTATGGTTCCGGCTCCTCGTGGTTCCGGAATTGTGGCTGCTAGGGTTCCGAAGAAGGTTTTGCAGTTTGCTGGTATTGATGATGTTTTCACCTCATCTAGGGGATCCACCAAAACCCTAGGAAACTTCGTCAAGGCCACTTTTGACTGTTTGTTGAAGACCTATGGTTTTCTGACACCTGAGTTCTGGAAGGAAACTAGGTTCTCCAAATCTCCATTCCAAGAGTACACTGATTTGTTGGCCAAGCCAACTGGAAAGACCTTGATCCTTGAGGAAGAAAGGGTGGAGGCTTGAAGTTTGAGTTTTGGTGTTGCTGGATAGTGATGGTTTGTTTTAAGCATCCTAGGAACTAGTAATTATATCTTTTGTTGCTAATGTTGTTCCCTTGAGTATTTAGTCACTATTTATAGTTTCTGGCATTTTTGTTTTTCAAGATTGTTTTTTTGATATTGAGTTTTAAATACCAAAGTTTTCTTCTTTATCTGCACTATGCTGTTCCATATTATAACTTCTGGTAGCTTAATAATTCAGTGAAAATCACGCATGTATTGCCATGAAC
TARGET=437,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2213
PRIMER_LEFT_PENALTY=0.131699
PRIMER_RIGHT_PENALTY=0.089612
PRIMER_LEFT_SEQUENCE=CCTCGAACAGATCTACCTCCAC
PRIMER_RIGHT_SEQUENCE=AGCTTAGCCAAGATAATCGCAC
PRIMER_LEFT=287,22
PRIMER_RIGHT=535,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=59.910
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.3902
PRIMER_LEFT_1_PENALTY=0.300539
PRIMER_RIGHT_1_PENALTY=0.089612
PRIMER_LEFT_1_SEQUENCE=GATCTACCTCCACTCTCTCCCA
PRIMER_RIGHT_1_SEQUENCE=AGCTTAGCCAAGATAATCGCAC
PRIMER_LEFT_1=296,22
PRIMER_RIGHT_1=535,22
PRIMER_LEFT_1_TM=59.699
PRIMER_RIGHT_1_TM=59.910
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=0.4171
PRIMER_LEFT_2_PENALTY=0.327468
PRIMER_RIGHT_2_PENALTY=0.089612
PRIMER_LEFT_2_SEQUENCE=AATCAAGGAGCACCAAATCATC
PRIMER_RIGHT_2_SEQUENCE=AGCTTAGCCAAGATAATCGCAC
PRIMER_LEFT_2=317,22
PRIMER_RIGHT_2=535,22
PRIMER_LEFT_2_TM=60.327
PRIMER_RIGHT_2_TM=59.910
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.4926
PRIMER_LEFT_3_PENALTY=0.402961
PRIMER_RIGHT_3_PENALTY=0.089612
PRIMER_LEFT_3_SEQUENCE=CAGCCTCGAACAGATCTACCTC
PRIMER_RIGHT_3_SEQUENCE=AGCTTAGCCAAGATAATCGCAC
PRIMER_LEFT_3=284,22
PRIMER_RIGHT_3=535,22
PRIMER_LEFT_3_TM=60.403
PRIMER_RIGHT_3_TM=59.910
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.5170
PRIMER_LEFT_4_PENALTY=0.427382
PRIMER_RIGHT_4_PENALTY=0.089612
PRIMER_LEFT_4_SEQUENCE=CTAGGTCGTCTCGTCAAGGAAG
PRIMER_RIGHT_4_SEQUENCE=AGCTTAGCCAAGATAATCGCAC
PRIMER_LEFT_4=252,22
PRIMER_RIGHT_4=535,22
PRIMER_LEFT_4_TM=60.427
PRIMER_RIGHT_4_TM=59.910
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_03890 Ps_contig_mira-and-tgicl-ass_franssen_31395
SEQUENCE=ACTCTTCGATTTCGCTCTCAAACCAAACAAATTAGACGGAAAAGGAACATACTAACCACAAGTCTCTTTCGCCCTCCGCCTCTCTCTCTCTGCCGCTCCCGCGCCGTCGCTCTCTCTCTCTCACTTGNANCRNCGTTAYCNACNCAGAGTCACAGGTATCTCACACCACTTTCAGTTCTTTTTTACTTTTTCAACCGAAGTCTCTTTCGCCCTCCGCCTCTCTCTCCGCCGCTCCCGCGCCGTCGCTCTCTCTCACACACTTGCACCGCCGTTCCTACCCAGAGTCACAGTTTTTTCTTGTGCTCAGTTGAGTGAGTTTAGAAGGAAGAGTGGTTTTATTTTTATCAATTTTATGGAGATCGACAGACCTGACGGAAGGACACCCAATCAACTGAGGCCCCTAGCTTGCTCTCATTCCGTCCTCCACCGAGCCCATGGCTCAGCCACATGGGCTCAAGGCGAAACCAAACTCCTTGCCGCAGTTTACGGACCTAAAGCCGGGGCCAAGAAGAACGAAAATCCCGAAAAAGCTTCCATTGAAGTTATTTGGAAGCCTAGTACAGGGCACGTTGGTCAAGCCGAGAGGGAGTATGAAATGATATTGAAGAAAACCTTGGAAAGCATCTGCATTCGTACCATATATCCCAACACCACCACTTCTGTTATAGTCCAGGTTGTCCATGATGATGGTGCTCTTCTTCCATGTGCAATAAACGCAGCATGTGCTGCACTCGTTGATGCCGGAATACCCCTAAGACATCTTGCTGTTGCAATATGTTGTTGTGTAACAGATAATAACAGTATTATATTGGACCCTTCCAAACAAGAAGAGAAGAAAGCGAAAGCATTTGCATATTTAGTTTTTCCAAATACAACTGTTACTGTCGTACCAGAGAAATCATTGCAAGTTGGCAGTGATCCCACGGCACACGGAATCATCACATCTGTTACACACGGTGCTATGTCAGTGGATAATTATCTTCATTGCCTAGAACGAGGGCGTGCCACAACTCAAAGGTTGTCTGAATTTTTGAGGAAGAACATCGAGCCAAAATCTATCCGCGAGGCATCCAAAGCTGGATGATTTACGAACATGTTGAATGTAACTTATTTGTATCCAATTTTTCCCTTACAAGGGTTGAAGAAGATGGAATCTTTTGTAGGACAATTTTTGTGACATATTTGCTCCGTTCCATGCCTGCTAACTATCTAAATTTCTGATTAGTTTTGTAGTTCTACAACATAACGAAGTTGCTATTACAATGAGTAAGATGATAATTTATGTATGATTATAAAATTGCCAGAGTCAAACTGTTCTTTTCATGA
TARGET=80,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03891 Ps_contig_mira-and-tgicl-ass_franssen_31395
SEQUENCE=ACTCTTCGATTTCGCTCTCAAACCAAACAAATTAGACGGAAAAGGAACATACTAACCACAAGTCTCTTTCGCCCTCCGCCTCTCTCTCTCTGCCGCTCCCGCGCCGTCGCTCTCTCTCTCTCACTTGNANCRNCGTTAYCNACNCAGAGTCACAGGTATCTCACACCACTTTCAGTTCTTTTTTACTTTTTCAACCGAAGTCTCTTTCGCCCTCCGCCTCTCTCTCCGCCGCTCCCGCGCCGTCGCTCTCTCTCACACACTTGCACCGCCGTTCCTACCCAGAGTCACAGTTTTTTCTTGTGCTCAGTTGAGTGAGTTTAGAAGGAAGAGTGGTTTTATTTTTATCAATTTTATGGAGATCGACAGACCTGACGGAAGGACACCCAATCAACTGAGGCCCCTAGCTTGCTCTCATTCCGTCCTCCACCGAGCCCATGGCTCAGCCACATGGGCTCAAGGCGAAACCAAACTCCTTGCCGCAGTTTACGGACCTAAAGCCGGGGCCAAGAAGAACGAAAATCCCGAAAAAGCTTCCATTGAAGTTATTTGGAAGCCTAGTACAGGGCACGTTGGTCAAGCCGAGAGGGAGTATGAAATGATATTGAAGAAAACCTTGGAAAGCATCTGCATTCGTACCATATATCCCAACACCACCACTTCTGTTATAGTCCAGGTTGTCCATGATGATGGTGCTCTTCTTCCATGTGCAATAAACGCAGCATGTGCTGCACTCGTTGATGCCGGAATACCCCTAAGACATCTTGCTGTTGCAATATGTTGTTGTGTAACAGATAATAACAGTATTATATTGGACCCTTCCAAACAAGAAGAGAAGAAAGCGAAAGCATTTGCATATTTAGTTTTTCCAAATACAACTGTTACTGTCGTACCAGAGAAATCATTGCAAGTTGGCAGTGATCCCACGGCACACGGAATCATCACATCTGTTACACACGGTGCTATGTCAGTGGATAATTATCTTCATTGCCTAGAACGAGGGCGTGCCACAACTCAAAGGTTGTCTGAATTTTTGAGGAAGAACATCGAGCCAAAATCTATCCGCGAGGCATCCAAAGCTGGATGATTTACGAACATGTTGAATGTAACTTATTTGTATCCAATTTTTCCCTTACAAGGGTTGAAGAAGATGGAATCTTTTGTAGGACAATTTTTGTGACATATTTGCTCCGTTCCATGCCTGCTAACTATCTAAATTTCTGATTAGTTTTGTAGTTCTACAACATAACGAAGTTGCTATTACAATGAGTAAGATGATAATTTATGTATGATTATAAAATTGCCAGAGTCAAACTGTTCTTTTCATGA
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03892 Ps_contig_mira-and-tgicl-ass_franssen_31402
SEQUENCE=TTACTTAGTTAACTACTTAGCCANGNATNTACANCACACTCCCATTTCCCTTCCCTCCCTCCCTACTTACTCTTCATTTACCCACTTCTCTCACAANCTTTCAGCCAAAAGACCCTCGTTCTCCCTGTCACAAAAGAATAGCGAAACAAACACTCCAATACATAGCTCAAATCAACCAAAGAACCCCACTAGTCCCACTAAACCTCATAGTCGATCTCGGAGGCCAGTTCCTCTGGGTCGACTGTGAAAATCAATACACTTCCTCTACATACCGCCCCGCGCGGTGCCGCTCCGCCCAATGCTCCCTAGCCAAAGCAAACGGCTGCGGCGATTGCTTCTCATCACCTAAACCCGGCTGCAACAACAACACATGTGGCCTACTTCCCGACAACACCGTCACCCACACCGCCACCAGCGGGGAACTCGCGGAAGACGTTTTATCAATCCAATCCACTAACGGCTTCAACCCAGGACAAACCGTCTCCGTTTCACGCTTCCTATTCTCATGTGCACCAACTTCCTTACTTAAAGGACTCGCAAGTGGTGCTTCTGGTATGGCCGGTCTTGGAAGAACCAAAATCGCTTTACCAACTCAACTGGCTTCCGCTTTTAGCTTCGATAGAAAATTTGCTTTTTGTTTTTCCTCTTCAAACGGCGTCGTTATCTTCGGTGATGGTCCTTACGGTTTCGCCGCCAATGTTGGTTCTGCCCCCAATGTTGTATATGATTCAAAATCTCTCACCTACACACCGCTATTAATCAACCCCGTTAGCACAGCTTCTGCTTTCTCACAAGGAGAGCCTTCAGCTGAGTATTTCATCGGAGTAAAAGCAATAAAAATAGATGAAAAAGTGGTTTCAGTTAACACGAGTTTGTTATCCATAGACAGTAACGGTGTTGGTGGAACAAAGATAAGTACGGTGGATCCTTATACAGTTCTAGAAGCTTCTATTTATAAAGCGGTTACAGAAGCTTTTGTGAAAGCTTCTATTGCAAGAAACATAACAAGGGTGGATTCGACTCCTCCTTTTGAATTTTGTTATAGTTTTGACAATTTGCCTGGGACGCCACTGGGTGCGGCTGTTCCTACAATTGAGCTTGTTTTAGAGAATGAAAATGTGGTTTGGGAGATGTTTGGTGCGAATTCGATGGTGAATATAAATGACGAGGTTTTGTGTCTTGGATTTGTGAACGGTGGTGAGAATCCGAGGAGTTCGATTGTGATCGGTGGGTATCAGTTGGAGAATCATCTTGTGCAGTTTGATTTGGCTGCCTCTAGATTGGGTTTTAGCTCCTCCCTGTTTGCAAGAAAAACTGATTGTTTTAGATTCAACTTCACCTCCACGGCTTAGAATCTTGAAGTTTGAATAATTAGTGTTGCATTCCTTCATAATAAATGCCTTTGAAGTTTATGTTTGAATTGATTGACAAGAGTATATAGTTTGATGGTGTAAACTAAAACAGAAATGTATGTTTTCCACTTTTTATATAAATCAAATACTTGTTTAGTTTAAAACCTAAAAAGT
TARGET=52,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7731
PRIMER_LEFT_PENALTY=1.677572
PRIMER_RIGHT_PENALTY=0.095574
PRIMER_LEFT_SEQUENCE=TACANCACACTCCCATTTCCCT
PRIMER_RIGHT_SEQUENCE=TCCGAGATCGACTATGAGGTTT
PRIMER_LEFT=29,22
PRIMER_RIGHT=220,22
PRIMER_LEFT_TM=61.678
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.75
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=1.7769
PRIMER_LEFT_1_PENALTY=1.677572
PRIMER_RIGHT_1_PENALTY=0.099308
PRIMER_LEFT_1_SEQUENCE=TACANCACACTCCCATTTCCCT
PRIMER_RIGHT_1_SEQUENCE=GACTAGTGGGGTTCTTTGGTTG
PRIMER_LEFT_1=29,22
PRIMER_RIGHT_1=193,22
PRIMER_LEFT_1_TM=61.678
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.75
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=1.7769
PRIMER_LEFT_2_PENALTY=1.677572
PRIMER_RIGHT_2_PENALTY=0.099308
PRIMER_LEFT_2_SEQUENCE=TACANCACACTCCCATTTCCCT
PRIMER_RIGHT_2_SEQUENCE=ACTAGTGGGGTTCTTTGGTTGA
PRIMER_LEFT_2=29,22
PRIMER_RIGHT_2=192,22
PRIMER_LEFT_2_TM=61.678
PRIMER_RIGHT_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.75
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=1.8869
PRIMER_LEFT_3_PENALTY=1.791325
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=TACANCACACTCCCATTTCCC
PRIMER_RIGHT_3_SEQUENCE=TCCGAGATCGACTATGAGGTTT
PRIMER_LEFT_3=29,21
PRIMER_RIGHT_3=220,22
PRIMER_LEFT_3_TM=60.791
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.75
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=1.8906
PRIMER_LEFT_4_PENALTY=1.791325
PRIMER_RIGHT_4_PENALTY=0.099308
PRIMER_LEFT_4_SEQUENCE=TACANCACACTCCCATTTCCC
PRIMER_RIGHT_4_SEQUENCE=ACTAGTGGGGTTCTTTGGTTGA
PRIMER_LEFT_4=29,21
PRIMER_RIGHT_4=192,22
PRIMER_LEFT_4_TM=60.791
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=164
=
PRIMER_SEQUENCE_ID=SSR_03893 Ps_contig_mira-and-tgicl-ass_franssen_31406
SEQUENCE=TAAAACTTGTGGCACTAGAGAGTATAGATGTGACTGTGGCACTCTCTTCTCAAGGCGTGATAGTTTCATTACTCATAGGGCATTTTGTGATGCACTAGCACATGAAAATGCAAGACACCCTTTCAACATGAACCCTTTAGCAACACATCTATTTTCCACAAACCACATAGGTTTAGCCCTAAACCAAAACCAACAAACATCCACAAACAACATCTTAACCCTAGGAAACATCCTTGTACACCAAAATATGAACATCATTTGATCTCACCTTTGCATCCATCATCATCATCATTTGTTCAACAACAACAACAACAATCACCACAACAACAATCAATGTCTTCTTCACCTTTCTT
TARGET=278,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2308
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=1.198499
PRIMER_LEFT_SEQUENCE=CAACATGAACCCTTTAGCAACA
PRIMER_RIGHT_SEQUENCE=GACATTGATTGTTGTTGTGGTG
PRIMER_LEFT=123,22
PRIMER_RIGHT=337,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=58.802
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=215
PRIMER_PAIR_PENALTY_1=1.2447
PRIMER_LEFT_1_PENALTY=0.095988
PRIMER_RIGHT_1_PENALTY=1.148716
PRIMER_LEFT_1_SEQUENCE=TGTGACTGTGGCACTCTCTTCT
PRIMER_RIGHT_1_SEQUENCE=GTGGTGATTGTTGTTGTTGTTG
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=321,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=58.851
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=1.2447
PRIMER_LEFT_2_PENALTY=0.095988
PRIMER_RIGHT_2_PENALTY=1.148716
PRIMER_LEFT_2_SEQUENCE=TGTGACTGTGGCACTCTCTTCT
PRIMER_RIGHT_2_SEQUENCE=GTTGTGGTGATTGTTGTTGTTG
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=324,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=58.851
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=1.2447
PRIMER_LEFT_3_PENALTY=0.095988
PRIMER_RIGHT_3_PENALTY=1.148716
PRIMER_LEFT_3_SEQUENCE=TGTGACTGTGGCACTCTCTTCT
PRIMER_RIGHT_3_SEQUENCE=GTTGTTGTGGTGATTGTTGTTG
PRIMER_LEFT_3=28,22
PRIMER_RIGHT_3=327,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=58.851
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=1.3320
PRIMER_LEFT_4_PENALTY=0.183296
PRIMER_RIGHT_4_PENALTY=1.148716
PRIMER_LEFT_4_SEQUENCE=AGCACATGAAAATGCAAGACAC
PRIMER_RIGHT_4_SEQUENCE=GTGGTGATTGTTGTTGTTGTTG
PRIMER_LEFT_4=96,22
PRIMER_RIGHT_4=321,22
PRIMER_LEFT_4_TM=60.183
PRIMER_RIGHT_4_TM=58.851
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_03894 Ps_contig_mira-and-tgicl-ass_franssen_31406
SEQUENCE=TAAAACTTGTGGCACTAGAGAGTATAGATGTGACTGTGGCACTCTCTTCTCAAGGCGTGATAGTTTCATTACTCATAGGGCATTTTGTGATGCACTAGCACATGAAAATGCAAGACACCCTTTCAACATGAACCCTTTAGCAACACATCTATTTTCCACAAACCACATAGGTTTAGCCCTAAACCAAAACCAACAAACATCCACAAACAACATCTTAACCCTAGGAAACATCCTTGTACACCAAAATATGAACATCATTTGATCTCACCTTTGCATCCATCATCATCATCATTTGTTCAACAACAACAACAACAATCACCACAACAACAATCAATGTCTTCTTCACCTTTCTT
TARGET=298,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2308
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=1.198499
PRIMER_LEFT_SEQUENCE=CAACATGAACCCTTTAGCAACA
PRIMER_RIGHT_SEQUENCE=GACATTGATTGTTGTTGTGGTG
PRIMER_LEFT=123,22
PRIMER_RIGHT=337,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=58.802
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=215
PRIMER_PAIR_PENALTY_1=1.3818
PRIMER_LEFT_1_PENALTY=0.183296
PRIMER_RIGHT_1_PENALTY=1.198499
PRIMER_LEFT_1_SEQUENCE=AGCACATGAAAATGCAAGACAC
PRIMER_RIGHT_1_SEQUENCE=GACATTGATTGTTGTTGTGGTG
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=337,22
PRIMER_LEFT_1_TM=60.183
PRIMER_RIGHT_1_TM=58.802
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=1.4081
PRIMER_LEFT_2_PENALTY=0.209637
PRIMER_RIGHT_2_PENALTY=1.198499
PRIMER_LEFT_2_SEQUENCE=CCCTTTCAACATGAACCCTTTA
PRIMER_RIGHT_2_SEQUENCE=GACATTGATTGTTGTTGTGGTG
PRIMER_LEFT_2=117,22
PRIMER_RIGHT_2=337,22
PRIMER_LEFT_2_TM=60.210
PRIMER_RIGHT_2_TM=58.802
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=221
PRIMER_PAIR_PENALTY_3=1.4664
PRIMER_LEFT_3_PENALTY=0.267873
PRIMER_RIGHT_3_PENALTY=1.198499
PRIMER_LEFT_3_SEQUENCE=AGACACCCTTTCAACATGAACC
PRIMER_RIGHT_3_SEQUENCE=GACATTGATTGTTGTTGTGGTG
PRIMER_LEFT_3=112,22
PRIMER_RIGHT_3=337,22
PRIMER_LEFT_3_TM=60.268
PRIMER_RIGHT_3_TM=58.802
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=1.8908
PRIMER_LEFT_4_PENALTY=0.692285
PRIMER_RIGHT_4_PENALTY=1.198499
PRIMER_LEFT_4_SEQUENCE=ACAAACCACATAGGTTTAGCCC
PRIMER_RIGHT_4_SEQUENCE=GACATTGATTGTTGTTGTGGTG
PRIMER_LEFT_4=157,22
PRIMER_RIGHT_4=337,22
PRIMER_LEFT_4_TM=59.308
PRIMER_RIGHT_4_TM=58.802
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_03895 Ps_contig_mira-and-tgicl-ass_franssen_31408
SEQUENCE=AACTATNTTATCATTGAACTAGATTTCTTTTATTTCTATACTATGAACCATGTATGTATGTTATATTTACCAAATTACAATTATGGATTGTTAATGAGAAATTGAGTTATGAAATTCAATGACAAGCTATATATGGATGACTTAATTACATGGGACAGAGAGATATGTTATCAGGAAAATAAGGGTCACTTGTCCTTGGTGGAAGTTGCCTCTGGAAGTTTGACTGAGGGGGACTTACTAACACCTTTCCTTGAGTATTCCCTCCATTTTCTAGCAGCAGCTGCAGCTCTTTTAGCTCTTTCTTTCACTTCCTCCTCTGAACTTTCGTAGTCTCTTGGTTTTCCGCGAAGATTGTCCCAGTATGAATTTGCTTTGTCATAACCTTTCTGCTTCATCATTCTCATTCTCGCTTGTGCCAAGAAGTTTAACTGCCTTTTCTCCCATCGGGTATTCGCCATTCTGAAAACATCGCTTAAAGCTGATCCCATTCCTGAAAGCATCAGAGCAACATTGACTCTTTCTATGCGGCGCATTTCGTCTTCAAGTAATGATAGTTGTGCAGCTGATGTCCAATGAATGCAGTCAACAGGGCAACTATCAATAGCCTGTTGAACTAAATCCGGGTTCCCGAACTGACTGTATACCCTGGCTCTTCCAAAGTCCTCCTCAATTTCAAACACATCACAGGCCACATTAGCACAGTTTTTGCAGCCTATGCAGCTGAACTCGTCCACAAAAACATGATCTTTTGGACTAGAATCATCCGCGAAAGGATTAATAGATGTCAAAGAATACCCGTGAATATCATCGTAAACCCTGCGCTGAACTGGATCACTGAGCACCTCATAGACTTCATTGATGAAGGTGCAGAAATTCGTTGTCTCAGGATCATTCCCACTCAAATCTGGATGGCTAAGTTTCATACAATTATAGTATGCTTTCT
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03896 Ps_contig_mira-and-tgicl-ass_franssen_31411
SEQUENCE=TTGAATTTGGAGGNRRGRGAAGAGAAAGGTGGTAGCTTTGTTTTGAGGTTGTGGGAATGGGGGATTTGGAGAAGCAGCAAGAGAAGGTGATGGTGTTGGAAGAAGGAAGGAAAGATGAAGAAGGTGAAGAGAATGAGAGGCTTTTGGAGGGTATGGCTGTTTTGGATTTTGATATGCTTTGTTCATCTGTTGCTTTACAAACTGCAAATGGAACTTGGGGGAAGCTTGGTGGTGGTGGTGAGGATGATGAGCATCGTGGTGAGGAGTTTGGTGGGGTTTTGAGGATGTGGGAAGGTGAACTTCTTGATTGCTTCGAGCATCGTCGAATCGCCCTTGAATCTGCATGTTGTCCCTGCTACCGATTCGGGAAGAACATGAAGCGTGCCGGTCTTGGTTCTTGTTATATTCAGGCGTTTGTATATTTTGTTGTTGCCATCTGTGCGCTTTTCAACTTCATAGCCTTTATCGTCACGAGACATCACTACTTTTTATACCTGGCAGTCACCTTCGTTGTTACTGGCGGAGCATATTTAGGATTCTACCGAACTCGAATGCGGAAGAAATTCAACATCAAGGGTAGCGATAGTTCGGTGGACGATTGTGCTTACCATTTTGTCTGTCCTTGTTGTACATTGTGTCAGGAGTCAAGAACACTAGAGATTAACAACGTTCAAGACGGCACTTGGCACGGCCGGGGCGACACCGTATGCATAGGTGGCATCAGGAACGGGAGTAAAGCACTCTCCGCTTTGATTCCTCCTCCTACCGAGTCCATCAAGCTTACGGATGAAATTTACACCTTATAGAAGAAATAGAACATAGAGGAGGAAGTTAAGAAACAATGGTTCTTCATCCAAATTGCATCCCTATCCATGTCAAGTTTATATCAAATTTCGCGACGAAAAGAGAATACAATGAAACGAAGGCTCTAACAAACNCCGTTCATAAGTCAGACATTGCTTCAAATTTCTTTGTCCAAGCAAACATTATTCT
TARGET=227,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03897 Ps_contig_mira-and-tgicl-ass_franssen_31415
SEQUENCE=ACACCATAAAATACATTCATTACAAACGTGCAGCTAATGAAAAAAACAAAAATGACCCAATTGAAATTTAACCACCCAAAAATGAGAAAAACCCTCTTTTAGGCTCTTCCTCAACCATAACAGCTTGCATACTATCCTGTTCGACAAGTCTCCAAGCAGCTTGTTCAAAAGCCAAACCAGCCAAAGTAGGAGGCTTATTCAAAACAAGAGGGTACCCTCTATTAGTACTTCTTATAACCTCACTATCCTCAGGTATAGCACCGAGCAAAGGCAACCCCAACATTTCTTGAACATCCAAAACCGACATCATATCTTCGCCTTTAATCATATCAGTCCTAACCCTATTCACTATCATCTTAATATCCCTAATCCCGTCACATTCCAATAACCCCGTTACTCTATCAGCATCCCTAAGAGCAGTTATATCAGGTGTAGTGATTAACACCGCCTCGTTCGCAGGCGTGATAGCTGTAATAAACCCTGCATCGATTCCGGCCGGACAATCAATGAGGATAAAGTCGGGGCTACGAGAACCTGTGGAACCATTTCTATTTCCACCGGTTACGTCTCCCCGGGCTTTTAAAGCTTCAACTAACCAAGTTAGGGCTTTTCCACCAAAGCCAATTGGGAGTTTAGATCTTGGTTTGGAAATACACAGAAGTTCAAAATTGGACCAACGTTTATCTCTAACCAAAGCTTGATCGAGTCGACATTCACCGTTGAGAACTTCCACAACAGTGTAATTGACTCGATTCTCGAGACCTAAAAGAAGGTCTAGGTTACGAAGTCCGACGTCAGCGTCGATTGCTACGACGGAGAAACCCAACCGGGCTAATGAGAGGCCGATGTTAGCGGTTGTTGTTGTTTTACCAACACCGCCTTTTCCGGATGTAACTACGACGACACGCGGTGTTTCGCCAGCGAGTTGTGGTTTGCGGTTCCATTGAAGGAGGGCTTTTGGGGTTAATGGAGGAAGGTAGGGTTTAGGGTGAATTGAGGTTGAATGACGGAGATGGGTGGTTGGAAGGATTTGGAGGTTGGCATTGGTGGTTCCGGGGAGGTGGTGGTGGAGTGATAGCATTGGGAGGGTTTCTGAGAGAGTGAGATGAAACTGAGTGTGAGCACTCAGGAGGAGAAGGTGATGAGAGAGAGAGAGAGAGAACAGTCTCGTGTTTGATCAAGAATCTCAACTT
TARGET=855,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2938
PRIMER_LEFT_PENALTY=0.185055
PRIMER_RIGHT_PENALTY=0.108707
PRIMER_LEFT_SEQUENCE=GTCTAGGTTACGAAGTCCGACG
PRIMER_RIGHT_SEQUENCE=ACCTCAATTCACCCTAAACCCT
PRIMER_LEFT=772,22
PRIMER_RIGHT=999,22
PRIMER_LEFT_TM=60.185
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.1000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=0.3523
PRIMER_LEFT_1_PENALTY=0.185055
PRIMER_RIGHT_1_PENALTY=0.167242
PRIMER_LEFT_1_SEQUENCE=GTCTAGGTTACGAAGTCCGACG
PRIMER_RIGHT_1_SEQUENCE=ATCTCCGTCATTCAACCTCAAT
PRIMER_LEFT_1=772,22
PRIMER_RIGHT_1=1013,22
PRIMER_LEFT_1_TM=60.185
PRIMER_RIGHT_1_TM=59.833
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.1000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.3527
PRIMER_LEFT_2_PENALTY=0.243965
PRIMER_RIGHT_2_PENALTY=0.108707
PRIMER_LEFT_2_SEQUENCE=CTAATGAGAGGCCGATGTTAGC
PRIMER_RIGHT_2_SEQUENCE=ACCTCAATTCACCCTAAACCCT
PRIMER_LEFT_2=831,22
PRIMER_RIGHT_2=999,22
PRIMER_LEFT_2_TM=60.244
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.4112
PRIMER_LEFT_3_PENALTY=0.243965
PRIMER_RIGHT_3_PENALTY=0.167242
PRIMER_LEFT_3_SEQUENCE=CTAATGAGAGGCCGATGTTAGC
PRIMER_RIGHT_3_SEQUENCE=ATCTCCGTCATTCAACCTCAAT
PRIMER_LEFT_3=831,22
PRIMER_RIGHT_3=1013,22
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=59.833
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.4128
PRIMER_LEFT_4_PENALTY=0.185055
PRIMER_RIGHT_4_PENALTY=0.227726
PRIMER_LEFT_4_SEQUENCE=GTCTAGGTTACGAAGTCCGACG
PRIMER_RIGHT_4_SEQUENCE=TCACCCTAAACCCTACCTTCCT
PRIMER_LEFT_4=772,22
PRIMER_RIGHT_4=991,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=60.228
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_03898 Ps_contig_mira-and-tgicl-ass_franssen_31415
SEQUENCE=ACACCATAAAATACATTCATTACAAACGTGCAGCTAATGAAAAAAACAAAAATGACCCAATTGAAATTTAACCACCCAAAAATGAGAAAAACCCTCTTTTAGGCTCTTCCTCAACCATAACAGCTTGCATACTATCCTGTTCGACAAGTCTCCAAGCAGCTTGTTCAAAAGCCAAACCAGCCAAAGTAGGAGGCTTATTCAAAACAAGAGGGTACCCTCTATTAGTACTTCTTATAACCTCACTATCCTCAGGTATAGCACCGAGCAAAGGCAACCCCAACATTTCTTGAACATCCAAAACCGACATCATATCTTCGCCTTTAATCATATCAGTCCTAACCCTATTCACTATCATCTTAATATCCCTAATCCCGTCACATTCCAATAACCCCGTTACTCTATCAGCATCCCTAAGAGCAGTTATATCAGGTGTAGTGATTAACACCGCCTCGTTCGCAGGCGTGATAGCTGTAATAAACCCTGCATCGATTCCGGCCGGACAATCAATGAGGATAAAGTCGGGGCTACGAGAACCTGTGGAACCATTTCTATTTCCACCGGTTACGTCTCCCCGGGCTTTTAAAGCTTCAACTAACCAAGTTAGGGCTTTTCCACCAAAGCCAATTGGGAGTTTAGATCTTGGTTTGGAAATACACAGAAGTTCAAAATTGGACCAACGTTTATCTCTAACCAAAGCTTGATCGAGTCGACATTCACCGTTGAGAACTTCCACAACAGTGTAATTGACTCGATTCTCGAGACCTAAAAGAAGGTCTAGGTTACGAAGTCCGACGTCAGCGTCGATTGCTACGACGGAGAAACCCAACCGGGCTAATGAGAGGCCGATGTTAGCGGTTGTTGTTGTTTTACCAACACCGCCTTTTCCGGATGTAACTACGACGACACGCGGTGTTTCGCCAGCGAGTTGTGGTTTGCGGTTCCATTGAAGGAGGGCTTTTGGGGTTAATGGAGGAAGGTAGGGTTTAGGGTGAATTGAGGTTGAATGACGGAGATGGGTGGTTGGAAGGATTTGGAGGTTGGCATTGGTGGTTCCGGGGAGGTGGTGGTGGAGTGATAGCATTGGGAGGGTTTCTGAGAGAGTGAGATGAAACTGAGTGTGAGCACTCAGGAGGAGAAGGTGATGAGAGAGAGAGAGAGAGAACAGTCTCGTGTTTGATCAAGAATCTCAACTT
TARGET=1144,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1003
PRIMER_LEFT_PENALTY=0.108707
PRIMER_RIGHT_PENALTY=1.991580
PRIMER_LEFT_SEQUENCE=AGGGTTTAGGGTGAATTGAGGT
PRIMER_RIGHT_SEQUENCE=TTCTTGATCAAACACGAGACTG
PRIMER_LEFT=978,22
PRIMER_RIGHT=1183,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=58.008
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=2.1588
PRIMER_LEFT_1_PENALTY=0.167242
PRIMER_RIGHT_1_PENALTY=1.991580
PRIMER_LEFT_1_SEQUENCE=ATTGAGGTTGAATGACGGAGAT
PRIMER_RIGHT_1_SEQUENCE=TTCTTGATCAAACACGAGACTG
PRIMER_LEFT_1=992,22
PRIMER_RIGHT_1=1183,22
PRIMER_LEFT_1_TM=59.833
PRIMER_RIGHT_1_TM=58.008
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=2.2193
PRIMER_LEFT_2_PENALTY=0.227726
PRIMER_RIGHT_2_PENALTY=1.991580
PRIMER_LEFT_2_SEQUENCE=GAGGAAGGTAGGGTTTAGGGTG
PRIMER_RIGHT_2_SEQUENCE=TTCTTGATCAAACACGAGACTG
PRIMER_LEFT_2=969,22
PRIMER_RIGHT_2=1183,22
PRIMER_LEFT_2_TM=60.228
PRIMER_RIGHT_2_TM=58.008
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=2.2193
PRIMER_LEFT_3_PENALTY=0.227726
PRIMER_RIGHT_3_PENALTY=1.991580
PRIMER_LEFT_3_SEQUENCE=AGGAAGGTAGGGTTTAGGGTGA
PRIMER_RIGHT_3_SEQUENCE=TTCTTGATCAAACACGAGACTG
PRIMER_LEFT_3=970,22
PRIMER_RIGHT_3=1183,22
PRIMER_LEFT_3_TM=60.228
PRIMER_RIGHT_3_TM=58.008
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=2.2505
PRIMER_LEFT_4_PENALTY=0.258908
PRIMER_RIGHT_4_PENALTY=1.991580
PRIMER_LEFT_4_SEQUENCE=GGTTTAGGGTGAATTGAGGTTG
PRIMER_RIGHT_4_SEQUENCE=TTCTTGATCAAACACGAGACTG
PRIMER_LEFT_4=980,22
PRIMER_RIGHT_4=1183,22
PRIMER_LEFT_4_TM=59.741
PRIMER_RIGHT_4_TM=58.008
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=204
=
PRIMER_SEQUENCE_ID=SSR_03899 Ps_contig_mira-and-tgicl-ass_franssen_31420
SEQUENCE=AGATCTAAGACAAGATAAACATACTACAAAGACAACATTAAGATAAGTTTGTACAAAACAAGAACAGAATTCATACATTAGAGGATCAGAATACCTTCTAGTACAAAAGTTAAAACATTAACATGACAGTTTACTCTTTTTTAGTGATTCTTAAAAGTCTACATTTTAAAAGTACAACATACAACATCTTAAAAAGGTTAATACTAGATATATTAAACAAAAGGTTCATAATCTCCTGCTCATAACTCAGTTAACTAGTGAAGATATGTAGCTTGGTACTGATAGCCATTTCCAAAGTAGTTTCCATGATTATGAGAGAAGTTATAATGTGCGTTGCCGAGGATAGGGGGACAATGATTGTCGGTTGGTCCTGCGTACATGTAAGGTCGGTCGTACACATATTGGGGGTACCTGTCAGCAGCAACTCTAGCATAGGAAGTTTTGTCAGAAGGGATGTTGTTAGTGACACGGGGACCATATCCTACACCGTTAGCACGAGGTCTCTTTGGTTGAGGCTTTGTCGCTTCAGTTTCCCTTTTTTTGTCAGCTTTCGCCTTCTCTAGTTGAACTACTCGTTTCACGAGAGGTTCCAGAGGATACTGCTCTTCGAGTTTATGTTCTTCAATGCACTTGATTACAGCTTTATGAGCAATCAGCTCTCGTTCATTAACCTCAATCTGTGCGGTAGGGGATGAGTTACCCCTTTTGACAGGTGAGCAAGAATTTCTAGCATCCTTCAAATAAGATTTCAGAAGTGAAACAGGAGAAAACTGTTTTGTCAGATCAAATGCAAAAGCCAAGTTAACCGCGTCAATTTGCCTCCCACTGTTCACCAAAACCTCAATTACACCAGGCATCTTCTCTGATAATCCAAGGCAACGGCATAAATCAGCAGTTTGGCGACGCCGAGACACCATTGGAATTAGCCTGGATAACTCTTCCTCATCAAAACCAGACGCAATATCAAAACTAGCTACGAGTTGCAAAAACGCATGAGCCTCCAAGGAATTCCCATTGCTAGCATCGATGTCAAGCGCATCCAATCTTGGCTTCCACTCTTCAGCAACTGCCTTTGCCCTATCCTTGATATCTTTGGAAATCACATTAGAAACGGAATGCAAATCGGTTAGGAAATCACTCAGACACTCCATCAGCATAATACAGGTCCGGCGAAGACCTAATAAGTTCGCATCTTTCTTAATATCCTGACTAGACACTTCCGTGCAGTAAAATCCTTCCAAAGAATCTAACACCAAACAGGCAGCATTAGGAGAAGCTCTTAACGCAAGTGGTATTTCCTCCCTTATAGCAGCAAGGTTCTTACGGTTATCAGATATGAATTTGTGAAGTCCAGAAACATCCATCTCTTTACAAAGTTTTACCAATTCTGGATAAGAGATCAATTCCACATTTCTATCTTCACGAGAAACTTTCACATCTTCTACGCGACCTTCCGTCACATTAGAAATAGCATCCGCATCCATCGGTTTCTCCTCCACACGTAATGCACATTGACCCCCATTGGAGACGATGCCCAAATCTTTTTGCGAAACCTTCCTGTGCTTTTCTCGAGCATTTACAATCGCAAATGTAGCAGCATCTCTTTTCTCTTGAAGCCTCTGCAATGTGGCTTGCTCCTGGGCAAAAATAGCCGCTTCCTGCTTCTTCAACATTTCACGAGCTTTCAGTGTTTTGTTCTCAAACACTTTTTCTTGATCTTCTAATTCGTGAAAGCGCCTCTTCAAGGATTTCTCAAGCCCATGAAAATGTTCCTCCAGTTCTTTCCATTTCATGTTAAGAGTAACCGCTCGATAACTTTCGAGTTCAGCAAACGCCTTCTGCAGCTGTTGTATCTTCGAAGACGTAGAATCCATCAGTGTAGCAACCGAGTTTGTTTCTTCCATTGCTAAGACGCTGATTGATCTGAAAGAAACAAGTACTGTAAAACAAAGAGAGGGAGAGGGAGAGATAGAGAGAAAGAGAGAGAGAGTTCTCAATCCATATTCTCAGTGCAACCCTCACATCAGAGAGAGAGAAATGAGAGAGAAGAATGAAGAAGAAGATGAAGAAAAGGAAGAGAGAGAGAGAGTGTGTGTGTGTGGTGTTTGTTAAATAAAATAAAATTGAGTAAAAGAGAGTCACAAAAAGCTGACTCCCCG
TARGET=1979,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0248
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=0.006915
PRIMER_LEFT_SEQUENCE=TCGATAACTTTCGAGTTCAGCA
PRIMER_RIGHT_SEQUENCE=CAAACACCACACACACACACTC
PRIMER_LEFT=1808,22
PRIMER_RIGHT=2107,22
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=60.007
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.1501
PRIMER_LEFT_1_PENALTY=0.017846
PRIMER_RIGHT_1_PENALTY=0.132262
PRIMER_LEFT_1_SEQUENCE=TCGATAACTTTCGAGTTCAGCA
PRIMER_RIGHT_1_SEQUENCE=TGAGGGTTGCACTGAGAATATG
PRIMER_LEFT_1=1808,22
PRIMER_RIGHT_1=2020,22
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=60.132
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=5.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.1558
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.132262
PRIMER_LEFT_2_SEQUENCE=AATGTTCCTCCAGTTCTTTCCA
PRIMER_RIGHT_2_SEQUENCE=TGAGGGTTGCACTGAGAATATG
PRIMER_LEFT_2=1765,22
PRIMER_RIGHT_2=2020,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.1558
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.132262
PRIMER_LEFT_3_SEQUENCE=TGTTCCTCCAGTTCTTTCCATT
PRIMER_RIGHT_3_SEQUENCE=TGAGGGTTGCACTGAGAATATG
PRIMER_LEFT_3=1767,22
PRIMER_RIGHT_3=2020,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=60.132
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=5.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.2388
PRIMER_LEFT_4_PENALTY=0.023556
PRIMER_RIGHT_4_PENALTY=0.215225
PRIMER_LEFT_4_SEQUENCE=AATGTTCCTCCAGTTCTTTCCA
PRIMER_RIGHT_4_SEQUENCE=GGGTTGCACTGAGAATATGGAT
PRIMER_LEFT_4=1765,22
PRIMER_RIGHT_4=2017,22
PRIMER_LEFT_4_TM=59.976
PRIMER_RIGHT_4_TM=60.215
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_03900 Ps_contig_mira-and-tgicl-ass_franssen_31420
SEQUENCE=AGATCTAAGACAAGATAAACATACTACAAAGACAACATTAAGATAAGTTTGTACAAAACAAGAACAGAATTCATACATTAGAGGATCAGAATACCTTCTAGTACAAAAGTTAAAACATTAACATGACAGTTTACTCTTTTTTAGTGATTCTTAAAAGTCTACATTTTAAAAGTACAACATACAACATCTTAAAAAGGTTAATACTAGATATATTAAACAAAAGGTTCATAATCTCCTGCTCATAACTCAGTTAACTAGTGAAGATATGTAGCTTGGTACTGATAGCCATTTCCAAAGTAGTTTCCATGATTATGAGAGAAGTTATAATGTGCGTTGCCGAGGATAGGGGGACAATGATTGTCGGTTGGTCCTGCGTACATGTAAGGTCGGTCGTACACATATTGGGGGTACCTGTCAGCAGCAACTCTAGCATAGGAAGTTTTGTCAGAAGGGATGTTGTTAGTGACACGGGGACCATATCCTACACCGTTAGCACGAGGTCTCTTTGGTTGAGGCTTTGTCGCTTCAGTTTCCCTTTTTTTGTCAGCTTTCGCCTTCTCTAGTTGAACTACTCGTTTCACGAGAGGTTCCAGAGGATACTGCTCTTCGAGTTTATGTTCTTCAATGCACTTGATTACAGCTTTATGAGCAATCAGCTCTCGTTCATTAACCTCAATCTGTGCGGTAGGGGATGAGTTACCCCTTTTGACAGGTGAGCAAGAATTTCTAGCATCCTTCAAATAAGATTTCAGAAGTGAAACAGGAGAAAACTGTTTTGTCAGATCAAATGCAAAAGCCAAGTTAACCGCGTCAATTTGCCTCCCACTGTTCACCAAAACCTCAATTACACCAGGCATCTTCTCTGATAATCCAAGGCAACGGCATAAATCAGCAGTTTGGCGACGCCGAGACACCATTGGAATTAGCCTGGATAACTCTTCCTCATCAAAACCAGACGCAATATCAAAACTAGCTACGAGTTGCAAAAACGCATGAGCCTCCAAGGAATTCCCATTGCTAGCATCGATGTCAAGCGCATCCAATCTTGGCTTCCACTCTTCAGCAACTGCCTTTGCCCTATCCTTGATATCTTTGGAAATCACATTAGAAACGGAATGCAAATCGGTTAGGAAATCACTCAGACACTCCATCAGCATAATACAGGTCCGGCGAAGACCTAATAAGTTCGCATCTTTCTTAATATCCTGACTAGACACTTCCGTGCAGTAAAATCCTTCCAAAGAATCTAACACCAAACAGGCAGCATTAGGAGAAGCTCTTAACGCAAGTGGTATTTCCTCCCTTATAGCAGCAAGGTTCTTACGGTTATCAGATATGAATTTGTGAAGTCCAGAAACATCCATCTCTTTACAAAGTTTTACCAATTCTGGATAAGAGATCAATTCCACATTTCTATCTTCACGAGAAACTTTCACATCTTCTACGCGACCTTCCGTCACATTAGAAATAGCATCCGCATCCATCGGTTTCTCCTCCACACGTAATGCACATTGACCCCCATTGGAGACGATGCCCAAATCTTTTTGCGAAACCTTCCTGTGCTTTTCTCGAGCATTTACAATCGCAAATGTAGCAGCATCTCTTTTCTCTTGAAGCCTCTGCAATGTGGCTTGCTCCTGGGCAAAAATAGCCGCTTCCTGCTTCTTCAACATTTCACGAGCTTTCAGTGTTTTGTTCTCAAACACTTTTTCTTGATCTTCTAATTCGTGAAAGCGCCTCTTCAAGGATTTCTCAAGCCCATGAAAATGTTCCTCCAGTTCTTTCCATTTCATGTTAAGAGTAACCGCTCGATAACTTTCGAGTTCAGCAAACGCCTTCTGCAGCTGTTGTATCTTCGAAGACGTAGAATCCATCAGTGTAGCAACCGAGTTTGTTTCTTCCATTGCTAAGACGCTGATTGATCTGAAAGAAACAAGTACTGTAAAACAAAGAGAGGGAGAGGGAGAGATAGAGAGAAAGAGAGAGAGAGTTCTCAATCCATATTCTCAGTGCAACCCTCACATCAGAGAGAGAGAAATGAGAGAGAAGAATGAAGAAGAAGATGAAGAAAAGGAAGAGAGAGAGAGAGTGTGTGTGTGTGGTGTTTGTTAAATAAAATAAAATTGAGTAAAAGAGAGTCACAAAAAGCTGACTCCCCG
TARGET=2026,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0248
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=0.006915
PRIMER_LEFT_SEQUENCE=TCGATAACTTTCGAGTTCAGCA
PRIMER_RIGHT_SEQUENCE=CAAACACCACACACACACACTC
PRIMER_LEFT=1808,22
PRIMER_RIGHT=2107,22
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=60.007
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.1392
PRIMER_LEFT_1_PENALTY=0.132262
PRIMER_RIGHT_1_PENALTY=0.006915
PRIMER_LEFT_1_SEQUENCE=CATATTCTCAGTGCAACCCTCA
PRIMER_RIGHT_1_SEQUENCE=CAAACACCACACACACACACTC
PRIMER_LEFT_1=1999,22
PRIMER_RIGHT_1=2107,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=60.007
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.2221
PRIMER_LEFT_2_PENALTY=0.215225
PRIMER_RIGHT_2_PENALTY=0.006915
PRIMER_LEFT_2_SEQUENCE=ATCCATATTCTCAGTGCAACCC
PRIMER_RIGHT_2_SEQUENCE=CAAACACCACACACACACACTC
PRIMER_LEFT_2=1996,22
PRIMER_RIGHT_2=2107,22
PRIMER_LEFT_2_TM=60.215
PRIMER_RIGHT_2_TM=60.007
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=0.2391
PRIMER_LEFT_3_PENALTY=0.232197
PRIMER_RIGHT_3_PENALTY=0.006915
PRIMER_LEFT_3_SEQUENCE=AAACAAAGAGAGGGAGAGGGAG
PRIMER_RIGHT_3_SEQUENCE=CAAACACCACACACACACACTC
PRIMER_LEFT_3=1944,22
PRIMER_RIGHT_3=2107,22
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=60.007
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.2707
PRIMER_LEFT_4_PENALTY=0.263824
PRIMER_RIGHT_4_PENALTY=0.006915
PRIMER_LEFT_4_SEQUENCE=GTTTCTTCCATTGCTAAGACGC
PRIMER_RIGHT_4_SEQUENCE=CAAACACCACACACACACACTC
PRIMER_LEFT_4=1893,22
PRIMER_RIGHT_4=2107,22
PRIMER_LEFT_4_TM=60.264
PRIMER_RIGHT_4_TM=60.007
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_03901 Ps_contig_mira-and-tgicl-ass_franssen_31420
SEQUENCE=AGATCTAAGACAAGATAAACATACTACAAAGACAACATTAAGATAAGTTTGTACAAAACAAGAACAGAATTCATACATTAGAGGATCAGAATACCTTCTAGTACAAAAGTTAAAACATTAACATGACAGTTTACTCTTTTTTAGTGATTCTTAAAAGTCTACATTTTAAAAGTACAACATACAACATCTTAAAAAGGTTAATACTAGATATATTAAACAAAAGGTTCATAATCTCCTGCTCATAACTCAGTTAACTAGTGAAGATATGTAGCTTGGTACTGATAGCCATTTCCAAAGTAGTTTCCATGATTATGAGAGAAGTTATAATGTGCGTTGCCGAGGATAGGGGGACAATGATTGTCGGTTGGTCCTGCGTACATGTAAGGTCGGTCGTACACATATTGGGGGTACCTGTCAGCAGCAACTCTAGCATAGGAAGTTTTGTCAGAAGGGATGTTGTTAGTGACACGGGGACCATATCCTACACCGTTAGCACGAGGTCTCTTTGGTTGAGGCTTTGTCGCTTCAGTTTCCCTTTTTTTGTCAGCTTTCGCCTTCTCTAGTTGAACTACTCGTTTCACGAGAGGTTCCAGAGGATACTGCTCTTCGAGTTTATGTTCTTCAATGCACTTGATTACAGCTTTATGAGCAATCAGCTCTCGTTCATTAACCTCAATCTGTGCGGTAGGGGATGAGTTACCCCTTTTGACAGGTGAGCAAGAATTTCTAGCATCCTTCAAATAAGATTTCAGAAGTGAAACAGGAGAAAACTGTTTTGTCAGATCAAATGCAAAAGCCAAGTTAACCGCGTCAATTTGCCTCCCACTGTTCACCAAAACCTCAATTACACCAGGCATCTTCTCTGATAATCCAAGGCAACGGCATAAATCAGCAGTTTGGCGACGCCGAGACACCATTGGAATTAGCCTGGATAACTCTTCCTCATCAAAACCAGACGCAATATCAAAACTAGCTACGAGTTGCAAAAACGCATGAGCCTCCAAGGAATTCCCATTGCTAGCATCGATGTCAAGCGCATCCAATCTTGGCTTCCACTCTTCAGCAACTGCCTTTGCCCTATCCTTGATATCTTTGGAAATCACATTAGAAACGGAATGCAAATCGGTTAGGAAATCACTCAGACACTCCATCAGCATAATACAGGTCCGGCGAAGACCTAATAAGTTCGCATCTTTCTTAATATCCTGACTAGACACTTCCGTGCAGTAAAATCCTTCCAAAGAATCTAACACCAAACAGGCAGCATTAGGAGAAGCTCTTAACGCAAGTGGTATTTCCTCCCTTATAGCAGCAAGGTTCTTACGGTTATCAGATATGAATTTGTGAAGTCCAGAAACATCCATCTCTTTACAAAGTTTTACCAATTCTGGATAAGAGATCAATTCCACATTTCTATCTTCACGAGAAACTTTCACATCTTCTACGCGACCTTCCGTCACATTAGAAATAGCATCCGCATCCATCGGTTTCTCCTCCACACGTAATGCACATTGACCCCCATTGGAGACGATGCCCAAATCTTTTTGCGAAACCTTCCTGTGCTTTTCTCGAGCATTTACAATCGCAAATGTAGCAGCATCTCTTTTCTCTTGAAGCCTCTGCAATGTGGCTTGCTCCTGGGCAAAAATAGCCGCTTCCTGCTTCTTCAACATTTCACGAGCTTTCAGTGTTTTGTTCTCAAACACTTTTTCTTGATCTTCTAATTCGTGAAAGCGCCTCTTCAAGGATTTCTCAAGCCCATGAAAATGTTCCTCCAGTTCTTTCCATTTCATGTTAAGAGTAACCGCTCGATAACTTTCGAGTTCAGCAAACGCCTTCTGCAGCTGTTGTATCTTCGAAGACGTAGAATCCATCAGTGTAGCAACCGAGTTTGTTTCTTCCATTGCTAAGACGCTGATTGATCTGAAAGAAACAAGTACTGTAAAACAAAGAGAGGGAGAGGGAGAGATAGAGAGAAAGAGAGAGAGAGTTCTCAATCCATATTCTCAGTGCAACCCTCACATCAGAGAGAGAGAAATGAGAGAGAAGAATGAAGAAGAAGATGAAGAAAAGGAAGAGAGAGAGAGAGTGTGTGTGTGTGGTGTTTGTTAAATAAAATAAAATTGAGTAAAAGAGAGTCACAAAAAGCTGACTCCCCG
TARGET=2076,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5715
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=1.439285
PRIMER_LEFT_SEQUENCE=CATATTCTCAGTGCAACCCTCA
PRIMER_RIGHT_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT=1999,22
PRIMER_RIGHT=2155,23
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=59.561
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=1.5976
PRIMER_LEFT_1_PENALTY=0.158317
PRIMER_RIGHT_1_PENALTY=1.439285
PRIMER_LEFT_1_SEQUENCE=TCAGTGTAGCAACCGAGTTTGT
PRIMER_RIGHT_1_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_1=1873,22
PRIMER_RIGHT_1=2155,23
PRIMER_LEFT_1_TM=59.842
PRIMER_RIGHT_1_TM=59.561
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=1.6545
PRIMER_LEFT_2_PENALTY=0.215225
PRIMER_RIGHT_2_PENALTY=1.439285
PRIMER_LEFT_2_SEQUENCE=ATCCATATTCTCAGTGCAACCC
PRIMER_RIGHT_2_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_2=1996,22
PRIMER_RIGHT_2=2155,23
PRIMER_LEFT_2_TM=60.215
PRIMER_RIGHT_2_TM=59.561
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=1.6715
PRIMER_LEFT_3_PENALTY=0.232197
PRIMER_RIGHT_3_PENALTY=1.439285
PRIMER_LEFT_3_SEQUENCE=AAACAAAGAGAGGGAGAGGGAG
PRIMER_RIGHT_3_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_3=1944,22
PRIMER_RIGHT_3=2155,23
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=59.561
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=1.7031
PRIMER_LEFT_4_PENALTY=0.263824
PRIMER_RIGHT_4_PENALTY=1.439285
PRIMER_LEFT_4_SEQUENCE=GTTTCTTCCATTGCTAAGACGC
PRIMER_RIGHT_4_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_4=1893,22
PRIMER_RIGHT_4=2155,23
PRIMER_LEFT_4_TM=60.264
PRIMER_RIGHT_4_TM=59.561
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_03902 Ps_contig_mira-and-tgicl-ass_franssen_31420
SEQUENCE=AGATCTAAGACAAGATAAACATACTACAAAGACAACATTAAGATAAGTTTGTACAAAACAAGAACAGAATTCATACATTAGAGGATCAGAATACCTTCTAGTACAAAAGTTAAAACATTAACATGACAGTTTACTCTTTTTTAGTGATTCTTAAAAGTCTACATTTTAAAAGTACAACATACAACATCTTAAAAAGGTTAATACTAGATATATTAAACAAAAGGTTCATAATCTCCTGCTCATAACTCAGTTAACTAGTGAAGATATGTAGCTTGGTACTGATAGCCATTTCCAAAGTAGTTTCCATGATTATGAGAGAAGTTATAATGTGCGTTGCCGAGGATAGGGGGACAATGATTGTCGGTTGGTCCTGCGTACATGTAAGGTCGGTCGTACACATATTGGGGGTACCTGTCAGCAGCAACTCTAGCATAGGAAGTTTTGTCAGAAGGGATGTTGTTAGTGACACGGGGACCATATCCTACACCGTTAGCACGAGGTCTCTTTGGTTGAGGCTTTGTCGCTTCAGTTTCCCTTTTTTTGTCAGCTTTCGCCTTCTCTAGTTGAACTACTCGTTTCACGAGAGGTTCCAGAGGATACTGCTCTTCGAGTTTATGTTCTTCAATGCACTTGATTACAGCTTTATGAGCAATCAGCTCTCGTTCATTAACCTCAATCTGTGCGGTAGGGGATGAGTTACCCCTTTTGACAGGTGAGCAAGAATTTCTAGCATCCTTCAAATAAGATTTCAGAAGTGAAACAGGAGAAAACTGTTTTGTCAGATCAAATGCAAAAGCCAAGTTAACCGCGTCAATTTGCCTCCCACTGTTCACCAAAACCTCAATTACACCAGGCATCTTCTCTGATAATCCAAGGCAACGGCATAAATCAGCAGTTTGGCGACGCCGAGACACCATTGGAATTAGCCTGGATAACTCTTCCTCATCAAAACCAGACGCAATATCAAAACTAGCTACGAGTTGCAAAAACGCATGAGCCTCCAAGGAATTCCCATTGCTAGCATCGATGTCAAGCGCATCCAATCTTGGCTTCCACTCTTCAGCAACTGCCTTTGCCCTATCCTTGATATCTTTGGAAATCACATTAGAAACGGAATGCAAATCGGTTAGGAAATCACTCAGACACTCCATCAGCATAATACAGGTCCGGCGAAGACCTAATAAGTTCGCATCTTTCTTAATATCCTGACTAGACACTTCCGTGCAGTAAAATCCTTCCAAAGAATCTAACACCAAACAGGCAGCATTAGGAGAAGCTCTTAACGCAAGTGGTATTTCCTCCCTTATAGCAGCAAGGTTCTTACGGTTATCAGATATGAATTTGTGAAGTCCAGAAACATCCATCTCTTTACAAAGTTTTACCAATTCTGGATAAGAGATCAATTCCACATTTCTATCTTCACGAGAAACTTTCACATCTTCTACGCGACCTTCCGTCACATTAGAAATAGCATCCGCATCCATCGGTTTCTCCTCCACACGTAATGCACATTGACCCCCATTGGAGACGATGCCCAAATCTTTTTGCGAAACCTTCCTGTGCTTTTCTCGAGCATTTACAATCGCAAATGTAGCAGCATCTCTTTTCTCTTGAAGCCTCTGCAATGTGGCTTGCTCCTGGGCAAAAATAGCCGCTTCCTGCTTCTTCAACATTTCACGAGCTTTCAGTGTTTTGTTCTCAAACACTTTTTCTTGATCTTCTAATTCGTGAAAGCGCCTCTTCAAGGATTTCTCAAGCCCATGAAAATGTTCCTCCAGTTCTTTCCATTTCATGTTAAGAGTAACCGCTCGATAACTTTCGAGTTCAGCAAACGCCTTCTGCAGCTGTTGTATCTTCGAAGACGTAGAATCCATCAGTGTAGCAACCGAGTTTGTTTCTTCCATTGCTAAGACGCTGATTGATCTGAAAGAAACAAGTACTGTAAAACAAAGAGAGGGAGAGGGAGAGATAGAGAGAAAGAGAGAGAGAGTTCTCAATCCATATTCTCAGTGCAACCCTCACATCAGAGAGAGAGAAATGAGAGAGAAGAATGAAGAAGAAGATGAAGAAAAGGAAGAGAGAGAGAGAGTGTGTGTGTGTGGTGTTTGTTAAATAAAATAAAATTGAGTAAAAGAGAGTCACAAAAAGCTGACTCCCCG
TARGET=2089,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5715
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=1.439285
PRIMER_LEFT_SEQUENCE=CATATTCTCAGTGCAACCCTCA
PRIMER_RIGHT_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT=1999,22
PRIMER_RIGHT=2155,23
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=59.561
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=1.5976
PRIMER_LEFT_1_PENALTY=0.158317
PRIMER_RIGHT_1_PENALTY=1.439285
PRIMER_LEFT_1_SEQUENCE=TCAGTGTAGCAACCGAGTTTGT
PRIMER_RIGHT_1_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_1=1873,22
PRIMER_RIGHT_1=2155,23
PRIMER_LEFT_1_TM=59.842
PRIMER_RIGHT_1_TM=59.561
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=1.6545
PRIMER_LEFT_2_PENALTY=0.215225
PRIMER_RIGHT_2_PENALTY=1.439285
PRIMER_LEFT_2_SEQUENCE=ATCCATATTCTCAGTGCAACCC
PRIMER_RIGHT_2_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_2=1996,22
PRIMER_RIGHT_2=2155,23
PRIMER_LEFT_2_TM=60.215
PRIMER_RIGHT_2_TM=59.561
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=1.6715
PRIMER_LEFT_3_PENALTY=0.232197
PRIMER_RIGHT_3_PENALTY=1.439285
PRIMER_LEFT_3_SEQUENCE=AAACAAAGAGAGGGAGAGGGAG
PRIMER_RIGHT_3_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_3=1944,22
PRIMER_RIGHT_3=2155,23
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=59.561
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=1.7031
PRIMER_LEFT_4_PENALTY=0.263824
PRIMER_RIGHT_4_PENALTY=1.439285
PRIMER_LEFT_4_SEQUENCE=GTTTCTTCCATTGCTAAGACGC
PRIMER_RIGHT_4_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_4=1893,22
PRIMER_RIGHT_4=2155,23
PRIMER_LEFT_4_TM=60.264
PRIMER_RIGHT_4_TM=59.561
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_03903 Ps_contig_mira-and-tgicl-ass_franssen_31420
SEQUENCE=AGATCTAAGACAAGATAAACATACTACAAAGACAACATTAAGATAAGTTTGTACAAAACAAGAACAGAATTCATACATTAGAGGATCAGAATACCTTCTAGTACAAAAGTTAAAACATTAACATGACAGTTTACTCTTTTTTAGTGATTCTTAAAAGTCTACATTTTAAAAGTACAACATACAACATCTTAAAAAGGTTAATACTAGATATATTAAACAAAAGGTTCATAATCTCCTGCTCATAACTCAGTTAACTAGTGAAGATATGTAGCTTGGTACTGATAGCCATTTCCAAAGTAGTTTCCATGATTATGAGAGAAGTTATAATGTGCGTTGCCGAGGATAGGGGGACAATGATTGTCGGTTGGTCCTGCGTACATGTAAGGTCGGTCGTACACATATTGGGGGTACCTGTCAGCAGCAACTCTAGCATAGGAAGTTTTGTCAGAAGGGATGTTGTTAGTGACACGGGGACCATATCCTACACCGTTAGCACGAGGTCTCTTTGGTTGAGGCTTTGTCGCTTCAGTTTCCCTTTTTTTGTCAGCTTTCGCCTTCTCTAGTTGAACTACTCGTTTCACGAGAGGTTCCAGAGGATACTGCTCTTCGAGTTTATGTTCTTCAATGCACTTGATTACAGCTTTATGAGCAATCAGCTCTCGTTCATTAACCTCAATCTGTGCGGTAGGGGATGAGTTACCCCTTTTGACAGGTGAGCAAGAATTTCTAGCATCCTTCAAATAAGATTTCAGAAGTGAAACAGGAGAAAACTGTTTTGTCAGATCAAATGCAAAAGCCAAGTTAACCGCGTCAATTTGCCTCCCACTGTTCACCAAAACCTCAATTACACCAGGCATCTTCTCTGATAATCCAAGGCAACGGCATAAATCAGCAGTTTGGCGACGCCGAGACACCATTGGAATTAGCCTGGATAACTCTTCCTCATCAAAACCAGACGCAATATCAAAACTAGCTACGAGTTGCAAAAACGCATGAGCCTCCAAGGAATTCCCATTGCTAGCATCGATGTCAAGCGCATCCAATCTTGGCTTCCACTCTTCAGCAACTGCCTTTGCCCTATCCTTGATATCTTTGGAAATCACATTAGAAACGGAATGCAAATCGGTTAGGAAATCACTCAGACACTCCATCAGCATAATACAGGTCCGGCGAAGACCTAATAAGTTCGCATCTTTCTTAATATCCTGACTAGACACTTCCGTGCAGTAAAATCCTTCCAAAGAATCTAACACCAAACAGGCAGCATTAGGAGAAGCTCTTAACGCAAGTGGTATTTCCTCCCTTATAGCAGCAAGGTTCTTACGGTTATCAGATATGAATTTGTGAAGTCCAGAAACATCCATCTCTTTACAAAGTTTTACCAATTCTGGATAAGAGATCAATTCCACATTTCTATCTTCACGAGAAACTTTCACATCTTCTACGCGACCTTCCGTCACATTAGAAATAGCATCCGCATCCATCGGTTTCTCCTCCACACGTAATGCACATTGACCCCCATTGGAGACGATGCCCAAATCTTTTTGCGAAACCTTCCTGTGCTTTTCTCGAGCATTTACAATCGCAAATGTAGCAGCATCTCTTTTCTCTTGAAGCCTCTGCAATGTGGCTTGCTCCTGGGCAAAAATAGCCGCTTCCTGCTTCTTCAACATTTCACGAGCTTTCAGTGTTTTGTTCTCAAACACTTTTTCTTGATCTTCTAATTCGTGAAAGCGCCTCTTCAAGGATTTCTCAAGCCCATGAAAATGTTCCTCCAGTTCTTTCCATTTCATGTTAAGAGTAACCGCTCGATAACTTTCGAGTTCAGCAAACGCCTTCTGCAGCTGTTGTATCTTCGAAGACGTAGAATCCATCAGTGTAGCAACCGAGTTTGTTTCTTCCATTGCTAAGACGCTGATTGATCTGAAAGAAACAAGTACTGTAAAACAAAGAGAGGGAGAGGGAGAGATAGAGAGAAAGAGAGAGAGAGTTCTCAATCCATATTCTCAGTGCAACCCTCACATCAGAGAGAGAGAAATGAGAGAGAAGAATGAAGAAGAAGATGAAGAAAAGGAAGAGAGAGAGAGAGTGTGTGTGTGTGGTGTTTGTTAAATAAAATAAAATTGAGTAAAAGAGAGTCACAAAAAGCTGACTCCCCG
TARGET=2090,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5715
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=1.439285
PRIMER_LEFT_SEQUENCE=CATATTCTCAGTGCAACCCTCA
PRIMER_RIGHT_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT=1999,22
PRIMER_RIGHT=2155,23
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=59.561
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=1.5976
PRIMER_LEFT_1_PENALTY=0.158317
PRIMER_RIGHT_1_PENALTY=1.439285
PRIMER_LEFT_1_SEQUENCE=TCAGTGTAGCAACCGAGTTTGT
PRIMER_RIGHT_1_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_1=1873,22
PRIMER_RIGHT_1=2155,23
PRIMER_LEFT_1_TM=59.842
PRIMER_RIGHT_1_TM=59.561
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=1.6545
PRIMER_LEFT_2_PENALTY=0.215225
PRIMER_RIGHT_2_PENALTY=1.439285
PRIMER_LEFT_2_SEQUENCE=ATCCATATTCTCAGTGCAACCC
PRIMER_RIGHT_2_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_2=1996,22
PRIMER_RIGHT_2=2155,23
PRIMER_LEFT_2_TM=60.215
PRIMER_RIGHT_2_TM=59.561
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=1.6715
PRIMER_LEFT_3_PENALTY=0.232197
PRIMER_RIGHT_3_PENALTY=1.439285
PRIMER_LEFT_3_SEQUENCE=AAACAAAGAGAGGGAGAGGGAG
PRIMER_RIGHT_3_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_3=1944,22
PRIMER_RIGHT_3=2155,23
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=59.561
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=1.7031
PRIMER_LEFT_4_PENALTY=0.263824
PRIMER_RIGHT_4_PENALTY=1.439285
PRIMER_LEFT_4_SEQUENCE=GTTTCTTCCATTGCTAAGACGC
PRIMER_RIGHT_4_SEQUENCE=GGAGTCAGCTTTTTGTGACTCTC
PRIMER_LEFT_4=1893,22
PRIMER_RIGHT_4=2155,23
PRIMER_LEFT_4_TM=60.264
PRIMER_RIGHT_4_TM=59.561
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_03904 Ps_contig_mira-and-tgicl-ass_franssen_31424
SEQUENCE=TTYATCAAAACTTTAGGCAACAAAATTCATTGATTATTAACATCCACTACATCTACAAGACTACAACAAACATCAAGAAATTAACATACTATCCTGCATATCCTTCTCATCCATAAGAACAAAAAAAATATATACAGAAGCCTAAAAAAAATACAAAACCATATTACATACTATACTATATTTATCATTAATTCATAGGTTAATGAAATTCAAGAGCCCTATACTATACTCAAGATTATTATTATTAATTAAAACACTTGAAATCAAATCATTCAACAGGAAGGAACGACGCCGTATCCATCATAAGACTCATCCTCAGCGATTAGATTCACATTCATATTCAAATCAGTGCGTTTTGCGAACCTTCCTTTAATTCTAGGTCTAGTTTCTGCATACGCTTTTCTCGATGCGTAACGAATTGTCTTCTCGAACCTTCTATTCTTTCTCTTCTCTCTGTACCTCATAACCCTAGCTTCTCTATCCGCCGCCGTGATTTGCACCGCCTCCGTCCTTCCGTAACCGCAGTATGACATCTCCGACATCACGTTTCCGTCTGGTACAACTCCAACCTCCAGCGAAGGCGACGATACGCTGTGATTGTAGTTGTAAGTAAAAGGTTTAGAAGTAGAGAAATCGTTGTTGATATCGGAGTAGTGTTCGGTGACGGTTTTGCTATGAGATTGCACCGGCACAACTCCGTCTGGGGTGCCGGGACTATGTTGCTCCGGCGAGTCAGTTTTGGCGTCAACACAGACGAAATTGAGGTCGGTAGCGTCGATTTCGGTGAATGAGTAGTGAGATGAGTTGAGATCGGGGCCTTTAGGGTTGGCGGGAGTTTGGAGGAGCCAGGAAGCAGCTTCGGCTTCTTCGGTGCTGACGTCAGCATCTGGATCGGATTCGGAGAAGAATTGTTGCTTGCTGTTGTGGTGGAAAGTGTTGAGCGGGATGCGTTCGTGGCGGCTAGCGAGGGGATTGGCGGAGTGGATGTCGTGGTCGCAGGAGACGCAGAGAGCAGCGGCGTCTGCTTTGCATGTGACGTGAGCAGGAGCTTGTTCACAGACTTCGCAGAGTGTGACGCGGTGATGGCGGGAGGCGAGTTTGTTGGCGGCGTGGATGTTGGAGTCACAGGTGATACAGAGGAAAGCTGAGTCAGAACGGCAGAATAGTGTTGCTTTTGTTGACTTGCAGGAATCACAGAGCTTAGTGGCCATTTGTTGTTTATTGAGTTAGTTAGAAGTTTGAGAAGTTGTGAGTGTATTGGCGAAGTGTTTTGGTTGGATTGGTAGTGTAGGGTNAAGAAATCCCCGA
TARGET=235,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03905 Ps_contig_mira-and-tgicl-ass_franssen_31426
SEQUENCE=CAAGGACGANGTTAAAAATGATTTAAAAATTTACTATATATTCTAAAAAAGCAATAAACACAAAGCAGATGACATCGTTAGAAGAAAATATGTATTGTGGAAGTTACGTCGAGCACACTCAATGGTGTGTCCCTTTCATTTTACAGTCACAGGGTCATACTTTAAAACTTGTTTTGCACTCGAAACACCAGAGTCTCTATTTAAACTTCCTATGCAACTTCTGGCAGGCTCCGCTAATCATGTACAAGCTCTTGTGTTCTTACAAAATGATGAGTTTCTCGTGAAAGGTCTTTCATTCTTGCACAGAGGGTTCTTTAGCTAGATCAAGCACTATAGGCTCCAATTTATCTGCTTCACCGTCTCCTTTGGGTATAATGAGTCCATAACCTCCTTCTTTTCTTTTGTATATAATATTAATCTCCCCAGTTTCTTCATTTCGAAATGCATAGAAGTCATGATGAACATTTTCCAACTGGTCAATTGCTTCAAACACGGTTAAGGGAGGTATGTCAAAGTATTTTGTTCGAACAACCTCTTCAATAAGTTGTTCTTCATCCTCCTTCTGTGGGGAAATTTCAACTTCATCCTCTTCATCCTCCGAAAGTAGTGGCAAAATAGGCTCCCTGAATTTCAACCTATTGGATCCCTTCATGTGACGACCATGATCAGTTTCTTTTTCCTTGATCTTTCTCAACTTTCTCTGAATGATCGAGGACACCAAATCTATACTCCCATAGGTAGTTTCAGCGTGTTCCTCGGCCCGCACAACCCCATGCCTCTTAGTAAATAAAGTCACCTCGCATCTGCGCGTTCTCGGCCCTCGGCCAAATTCTCCTCCTCCGCGAGTAGATAGCCTGACATCAACTTCCCTAACAAGGTAGCTGTGCTTCTGAACTGCTCTCCCCACTTTGTCCTCGATGTGCTGCTTCACCGCATCATTAAGCTCCATATTTTTACCCTGGATGATTAATTTGACGGAGGAGAGTGAACCGTCCCACGACATGCGAATAACGCGGTGTTTTGTTGGTGGTGGTGGTGTTGCAGCATAGTTGAATTTGAGAAATTGTTTAGTGATTTGTCCTTTGAAGAATGTTGGGGAGGGTAGTAGCTTGGTCCTGGAGGAGATTTGAGGAGATAGAGAGAGTACAGAAAGGCGGCGAGTGGGAGAAGGGGAAGATGGGAGAAAGTTAGTTTGGAAGTTGAAGTGAGTAGTCGCCATTGGCATTTGAATCCCCNCGTNCTGCTACGTTTGTATTACCTTACTTTGTTCTTTT
TARGET=1025,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1037
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.099308
PRIMER_LEFT_SEQUENCE=GCCTGACATCAACTTCCCTAAC
PRIMER_RIGHT_SEQUENCE=CTCCTCCAGGACCAAGCTACTA
PRIMER_LEFT=852,22
PRIMER_RIGHT=1123,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.901
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=4.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.1311
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.099308
PRIMER_LEFT_1_SEQUENCE=GATAGCCTGACATCAACTTCCC
PRIMER_RIGHT_1_SEQUENCE=CTCCTCCAGGACCAAGCTACTA
PRIMER_LEFT_1=848,22
PRIMER_RIGHT_1=1123,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=4.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.1345
PRIMER_LEFT_2_PENALTY=0.035219
PRIMER_RIGHT_2_PENALTY=0.099308
PRIMER_LEFT_2_SEQUENCE=TAGCTGTGCTTCTGAACTGCTC
PRIMER_RIGHT_2_SEQUENCE=CTCCTCCAGGACCAAGCTACTA
PRIMER_LEFT_2=878,22
PRIMER_RIGHT_2=1123,22
PRIMER_LEFT_2_TM=59.965
PRIMER_RIGHT_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=4.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=0.1743
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.099308
PRIMER_LEFT_3_SEQUENCE=ACCCTGGATGATTAATTTGACG
PRIMER_RIGHT_3_SEQUENCE=CTCCTCCAGGACCAAGCTACTA
PRIMER_LEFT_3=955,22
PRIMER_RIGHT_3=1123,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.901
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=4.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=0.2297
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.099308
PRIMER_LEFT_4_SEQUENCE=AGCCTGACATCAACTTCCCTAA
PRIMER_RIGHT_4_SEQUENCE=CTCCTCCAGGACCAAGCTACTA
PRIMER_LEFT_4=851,22
PRIMER_RIGHT_4=1123,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=4.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_03906 Ps_contig_mira-and-tgicl-ass_franssen_31430
SEQUENCE=ATTCAAAACAAGAAACAGAAGCCAGTGATAATAGGCTTATAATGAAGTTGAAAAAAGAGGTAAAAGCTCTCAAGCAAGAACTTGATAAGGGAATGAGTTATGATGAGAAGTTGACAGAGAAAGAAAATCTTATTGAACAACTTAATGTCGAACTTGAAACCTCAAGGACGGCAGAATCTTATGCCCGCAGTCTGCTAGAGGAGTGGAAGAAGAAGGTTGAGGAACTGAAAATGAGGGTTGAAGAATCAAACAAGTTGGAGAGATCCGCATCAGAATCCTTGGAATCTGTAATGAAACAGCTAGAAGGAAGCAATGATCTGTTGCATGATGCAGAGTCTGTAGTTGGTCCTCTTAAAGAGAAGGTGGGATTGTTAGAAATGACTATTGGGAGACAAAAAACCGATTTTGAGGAATCTGAACGTCGCCTTCTTATGGCTAAGGAAGAAAATCTAGAAATTTCAAAGAAGATTGAGTCTCTCAAATCCGAACTTGAGACAGTGAAGGAAGAGAAAGAACAGGCTTTGAACAATGAACATCTTGCAGCCTCTAGTGTGCAGACTCTATTAGAAGAGAAAAACAAACTTATCAATGACTTGGAGAAGTCTAAGGAAGAAGAAGAAAAGAGTAAAAAGGCAATGGAAAGCTTAGCTTCTGCATTACATGAAATATCTGCGGAATCTAGAGAAGCTAAAGAAAATCTACTGGGCAATCAAGCTGAAAGGCAAAGCTATGAGAACCAGATTGAAGATTTAAAGTTAGTCTTAAAAGGTACTAACGAAAAATATGAGTCGATGCTTGACGATGCACGACATGAAATTGATGTTCTTATATTCAGTATTGAAAGCTCGAAGAATGTCTTTGAGAACTCAAAGGCTGAGTGGGAGCGGAGAGAGCTTCATCTAGTCAGCAGCCTGAAGAAAACTGAAGAAGAGAATGCGGCCACGGAAAAAGAAATAAATAGATTGGTTTATTTGCTTAAAGAAGTTGAGGAAGAAGCAAATTCCAACAGGGAGGAAGAAGCTCGGTTGAAGGAAAATCTGAATGAAGTTGAGGCTGAGGCAATCCATCTGCAGGAAGCTTTGAAGGAAGTAACATCTGAAAATGTTAAATTGAAGGAAAATATATTGGATAAAGAAAATAGAAATGACAAGAATTGTTTTTACAAAACGG
TARGET=609,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2500
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.247716
PRIMER_LEFT_SEQUENCE=TGAAGGAAGAGAAAGAACAGGC
PRIMER_RIGHT_SEQUENCE=ATCTGGTTCTCATAGCTTTGCC
PRIMER_LEFT=498,22
PRIMER_RIGHT=741,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.752
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.3671
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.364827
PRIMER_LEFT_1_SEQUENCE=TGAAGGAAGAGAAAGAACAGGC
PRIMER_RIGHT_1_SEQUENCE=GCTTGATTGCCCAGTAGATTTT
PRIMER_LEFT_1=498,22
PRIMER_RIGHT_1=714,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=59.635
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.4724
PRIMER_LEFT_2_PENALTY=0.385416
PRIMER_RIGHT_2_PENALTY=0.087031
PRIMER_LEFT_2_SEQUENCE=CAGGCTTTGAACAATGAACATC
PRIMER_RIGHT_2_SEQUENCE=CTGGTTCTCATAGCTTTGCCTT
PRIMER_LEFT_2=515,22
PRIMER_RIGHT_2=739,22
PRIMER_LEFT_2_TM=59.615
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=0.4737
PRIMER_LEFT_3_PENALTY=0.225996
PRIMER_RIGHT_3_PENALTY=0.247716
PRIMER_LEFT_3_SEQUENCE=CAAATCCGAACTTGAGACAGTG
PRIMER_RIGHT_3_SEQUENCE=ATCTGGTTCTCATAGCTTTGCC
PRIMER_LEFT_3=478,22
PRIMER_RIGHT_3=741,22
PRIMER_LEFT_3_TM=59.774
PRIMER_RIGHT_3_TM=59.752
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=0.5108
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.508459
PRIMER_LEFT_4_SEQUENCE=TGAAGGAAGAGAAAGAACAGGC
PRIMER_RIGHT_4_SEQUENCE=TAGCTTTGCCTTTCAGCTTGAT
PRIMER_LEFT_4=498,22
PRIMER_RIGHT_4=729,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=60.508
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_03907 Ps_contig_mira-and-tgicl-ass_franssen_31432
SEQUENCE=TTTCCAGTTCACCGGAGNAACCGCCGCCAGTCTGTATAGGATTCANTTTCCAGCCGCCATCACTCTCAAGGATCGCTACAATATAGCAACAAGATCAGACAAGATTCTATGCGTTGTTATGATAGTCCTCGCCGTCGCCTCGAATGTTGTGGCTATATACAGCAATGCATTTGGATTGATCACGAAGAGCAATACTTCGCGCGAATGATGGTCATGGTTCTTGTCGAAAGGAACTGAATTGGCCGAGTAAATTCAGTTAGTCAGAAAATCAGGGTTTTCTGAGAGGGTCTCATGAGTCGTCGTCGTCGATTTTGAATAAACGTAGAAGCTACTACCCTGTGTACAAATTTGCTTCGAAAGGTGTCGAGGCCTGCAACATGTATTCGGTTTCGTTTGTAATTTGCGAATTCGGTTATAGTTATTGTGGTTTTTCTTTTGAGTAATAAATACTCTCTTTCCTTGATTTGTTTTAAAGATGTAATAGAACTTTTTGCTGGCTTCTGTATGTATTGTATTGTGTTGAGTGAAGGAATGAAACTATGTATTCTTGAATTGGACAAGAGAAAATTGCTATNAG
TARGET=296,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1808
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.165541
PRIMER_LEFT_SEQUENCE=TCACTCTCAAGGATCGCTACAA
PRIMER_RIGHT_SEQUENCE=TCGAAGCAAATTTGTACACAGG
PRIMER_LEFT=59,22
PRIMER_RIGHT=356,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=60.166
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=0.3175
PRIMER_LEFT_1_PENALTY=0.151911
PRIMER_RIGHT_1_PENALTY=0.165541
PRIMER_LEFT_1_SEQUENCE=TGCGTTGTTATGATAGTCCTCG
PRIMER_RIGHT_1_SEQUENCE=TCGAAGCAAATTTGTACACAGG
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=356,22
PRIMER_LEFT_1_TM=60.152
PRIMER_RIGHT_1_TM=60.166
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.5966
PRIMER_LEFT_2_PENALTY=0.431057
PRIMER_RIGHT_2_PENALTY=0.165541
PRIMER_LEFT_2_SEQUENCE=CATTTGGATTGATCACGAAGAG
PRIMER_RIGHT_2_SEQUENCE=TCGAAGCAAATTTGTACACAGG
PRIMER_LEFT_2=167,22
PRIMER_RIGHT_2=356,22
PRIMER_LEFT_2_TM=59.569
PRIMER_RIGHT_2_TM=60.166
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.6251
PRIMER_LEFT_3_PENALTY=0.459539
PRIMER_RIGHT_3_PENALTY=0.165541
PRIMER_LEFT_3_SEQUENCE=ATTTGGATTGATCACGAAGAGC
PRIMER_RIGHT_3_SEQUENCE=TCGAAGCAAATTTGTACACAGG
PRIMER_LEFT_3=168,22
PRIMER_RIGHT_3=356,22
PRIMER_LEFT_3_TM=60.460
PRIMER_RIGHT_3_TM=60.166
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.7626
PRIMER_LEFT_4_PENALTY=0.348961
PRIMER_RIGHT_4_PENALTY=0.413619
PRIMER_LEFT_4_SEQUENCE=AATCAGGGTTTTCTGAGAGGGT
PRIMER_RIGHT_4_SEQUENCE=ACAGAAGCCAGCAAAAAGTTCT
PRIMER_LEFT_4=266,22
PRIMER_RIGHT_4=503,22
PRIMER_LEFT_4_TM=60.349
PRIMER_RIGHT_4_TM=59.586
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_03908 Ps_contig_mira-and-tgicl-ass_franssen_31433
SEQUENCE=GGTTGACGNAGAAGCAAAAGAGTGAAGATGGATTGCGAGAGGAATGGAATTTTTGGACGAAGATGCGAAACCGAAGTTCCTGTTTCAGTCCGGTGCACCCGCGCCCACTGTATCACAGCCTTCCGAGAAACCAACGAAACCGTTCATCGCTGTAACCGTTGCTCTCTCTTCAATCTTCTTCGCTCTCGCTATCTTCGTCTTCCCATCGGAACCTTACAGATCGATTCTCTTCTGGTTCGCACTATCACTTCTCCTTGGTCCTTTCGCTCCTAGCTCAATCACTGGCGGTGATTTCCGCGTCGGCCGTGGTCATGTCGTTAACTTCCCCGATCAGGAAGCCTCAGCTGATGATGATGACGTCAGGAAAAGATCTCAGCAGCGCAGATCTAAGCCGCGGAGATCGGAGGATGTTGCGTCCGTAGTTCTTCCGGCGCCGGTAGTTTCAAACGGCAGTGCTTTGAAGAATCGAAATGGAAACGGTGCTTCATTGACGGCGGCGGTGGTGGAGGAGGAGAAGGAATGGAACGAGGACGACATTGAGATTCTGAAGAAGCAAATTGTGAAACATCCGGTGGGGAAACCAGGACGATGGGAGGTGATAGCGGAGGCATTCGGAGGGCGGCACAAAGCAGAGAGCGTGGTGAAGAAGGTGAAGGAGTTAGGGGAGAAGAAGGTTGATGATTCGGATTCGTATGAGCAGTTTCTGAAGAAGAGAAAAGCGTTGGATAAGAGACTTGTTGAAGAAGGAGGAGAATTAGCAACGGTTGAGAAAGTTGAGAGTGTTTGGAGTTCAAATGAGGACATTGCTTTGTTGAATGCTCTGAAAGCATTTCCGAAAGATGTTGCAATGAGATGGGAGAAAGTTGCTGCTGCTGTTCCCGGAAAATCGAAAGCTGCTTGTATGAAAAGAATGGCTGAATTGAAGAAAGGGTTTCGGAATGCAAAATCTGGATCCGAAGTTTAGATTCAAAATTCTCAAGATCTGTTATCTTGGTATATGGATTAATACTTGTAACTCATGCGTTTTTTTTTGTTGTATTCTAGATATTTGAGTTATGAATTGTATCGGAAAATTGTTTGGATGAGTCATGAGTCATTCTTTGCTTGTTCATCAAGTTACTATGTGAAATCTAACTTCATTGAATAAATAGAATGAACAAGATGTATTACAAAGTTATGTTTCTATAGTGCAAATATATGTTTACTTGATACNAAAG
TARGET=346,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5407
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.525419
PRIMER_LEFT_SEQUENCE=TCTCTTCTGGTTCGCACTATCA
PRIMER_RIGHT_SEQUENCE=GCTGCTGAGATCTTTTCCTGAC
PRIMER_LEFT=225,22
PRIMER_RIGHT=379,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=60.525
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.5443
PRIMER_LEFT_1_PENALTY=0.018835
PRIMER_RIGHT_1_PENALTY=0.525419
PRIMER_LEFT_1_SEQUENCE=CCGTTGCTCTCTCTTCAATCTT
PRIMER_RIGHT_1_SEQUENCE=GCTGCTGAGATCTTTTCCTGAC
PRIMER_LEFT_1=155,22
PRIMER_RIGHT_1=379,22
PRIMER_LEFT_1_TM=60.019
PRIMER_RIGHT_1_TM=60.525
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=0.5776
PRIMER_LEFT_2_PENALTY=0.052187
PRIMER_RIGHT_2_PENALTY=0.525419
PRIMER_LEFT_2_SEQUENCE=TGTAACCGTTGCTCTCTCTTCA
PRIMER_RIGHT_2_SEQUENCE=GCTGCTGAGATCTTTTCCTGAC
PRIMER_LEFT_2=150,22
PRIMER_RIGHT_2=379,22
PRIMER_LEFT_2_TM=60.052
PRIMER_RIGHT_2_TM=60.525
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.6071
PRIMER_LEFT_3_PENALTY=0.081670
PRIMER_RIGHT_3_PENALTY=0.525419
PRIMER_LEFT_3_SEQUENCE=GCTGTAACCGTTGCTCTCTCTT
PRIMER_RIGHT_3_SEQUENCE=GCTGCTGAGATCTTTTCCTGAC
PRIMER_LEFT_3=148,22
PRIMER_RIGHT_3=379,22
PRIMER_LEFT_3_TM=60.082
PRIMER_RIGHT_3_TM=60.525
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=232
PRIMER_PAIR_PENALTY_4=0.6271
PRIMER_LEFT_4_PENALTY=0.101680
PRIMER_RIGHT_4_PENALTY=0.525419
PRIMER_LEFT_4_SEQUENCE=TCTTCTTCGCTCTCGCTATCTT
PRIMER_RIGHT_4_SEQUENCE=GCTGCTGAGATCTTTTCCTGAC
PRIMER_LEFT_4=173,22
PRIMER_RIGHT_4=379,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=60.525
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_03909 Ps_contig_mira-and-tgicl-ass_franssen_31444
SEQUENCE=CGGAAGTATTTAGTNTTTTACCTTGTAGTTAACTAAATAGTCTGACTTAAAATAAAGTAAGTAAGTAATTCTTAATAGCCTCCACTTCCTCGACTCATTTGTTATTTTCCTTGATCCCATCCATTTCAACTGAGAATCATATCCATTACTTTTATTCTTCTGAGGATAATCCACATTATCATAATCATCTTCAATAAACTTCTTCCAAAACCAATGCTGCTTCCACACTCTCTCTGTCATTTCTTCAATTGGTATGTTCTTCGTCTCAGGCACCAAGAAAAACACAAAAGCAGACATAACCAAAACCCAGCTAGAGAAGAACAAGAATATACCAAACTTGAAATGGCACAACATGGACAGAAAAGCTTGAGCAATTACAAAGGTGAAAAGCAAGTTGACACAAACAGTTACACTTTGTCCAGCCGAACGTGTCTCCAATGGAAATGTCTCACTTGGTATAAGCCAACCAAGTGGACCCCAAGACCAAGCAAAAGCAGACACAAATGTGCATACCATGATGACAACAAAGATTGCATAGCCTTTTGAAAGATCCGAGGAATGATCTGTTACTTTGATTCCAAGAAGGATTGCAATGATCAATTGAGAGAAGAACATTTGCACACCGGCTTCTAATARCAACATGCGACGACCAAGTTTGTCGACCGTGTAAATAGACACAATTGTAGAGAGGACGTTGACAGCTCCGGTTATCACGGCGGAATAGAGTGAAGCATCATTCTTAAATCCTAACGTGTTGAATAACACCGGCGCGTAAAACATGATCGCGTTGATGCCAGTGAATTGTTGGAATATTTGCAAGGCGATAGAAATGACGAGTTGAGGACGGTTCCTGCGCTTGAGAAGGTTCCTAAAAGGGTGTTTAACCTCTTTAGCAACGCGACTTGCCTCGACAAGTTCTAAGAACTCTGGTTCAACATTATCAGTACCACGAATCTTTTTGAGTACAGCTTTTCCTTCATCCAAGCGACCGCGCTCAATGAGACTGTTGGGAGTGTCTACAACCAAGAGGGCGCCAACCGTCAGGAGAAGCGCCGGGATCCCGGCCAAACCAAGAGATAGTCTCCATCCCCAACCACCTTCGATTTTGTTGGTGCCATAATTAACAAGATTAGCAAACAAAATGCCAATCGTGACATTAAGTTGGAAAAGTATATTCAAAGCACCACGTATTCTTGAAGGAGCAATTTCTGAAAGAAACACTGGCACAGCCTGATTAGCAAAACCAACACCACAACCAAGTAAGATTCTCCCAACGATAAGCATGGCAAGATTTTGAGCAGCAGCATTGAAGATAACTCCAGCAATGAAGAAAATACCAGCAATCAACATGGTGAGTCTTCTTCCCAAAGTCCTAGTTGTATAGGAAGCAAAGAAAGTTGATGTTAAACCGGCTAAATACAGAGACGATGTAAAAAGTTGAAGTCCCTGGTTGTCATATTTGCAGTAGTTACTGTTAATCTCTTTCTCCAACACTGTCTTTTTGTATACAGCAGGGAAGAATTTCTTCAAAAATGGTGGCATAGATGTCACACCACCTGAAACACCGACGTCGTAACCGAACATGAGACCCCCGGTGGCAGCCATGATACATGAAATAATAACGATCGGAGTTATCTTCGCTTCGAACTCGCCGCCGCCTGCACTGGTGGCGAAACCTCCTCCGGCCATAGCTTGAAAAATCTAAATTAGATCTTAGTTGTATATCACAATCCACATGCAATGGTGGAGTGAGAAAAAGAATAGAGAAATAGGAGTCCCCG
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03910 Ps_contig_mira-and-tgicl-ass_franssen_31453
SEQUENCE=ACGTATTTATATATTTATAATATATAAATAAACATGTGTATTTTAGAGTATCNAAGTATATCCATTGATTGATTGTACGAGCAGGAAAGAGTCCACTGTTTCCGGACACCTTCCTACTTTTGTTTCTTCTTCAACTAACTCATCCACTCTCTACTCCACTTCAACTTTCTCCTAACGATTTTCCCTATGGAGCACAAGTATTTGTGGGTGGCTTGCTTTTTGTGGGCTTTAACATGCTCACTTCTTCCTTCTTTCTCTTATGGATTTATGAGAATTGATTTGAAGAAGAGAACTCTTGATCTTGATAGTATTAAAGCTGCTAGAATGGTAAGAGAAAAGCTAAGGTTAGGCAGACCCGTGTTGGGCGCATATGATCAGTACACCGGGAAACTAACCGATGATGATGCCATAGTACCTTTGAAGAATTATTTGGACGCACAATATTATGGAGAGATTGGAATTGGCTCACCCCCTCAGACCTTTAATGTCATATTTGATACCGGTAGTTCAAACCTTTGGGTTCCATCGTCCAAGTGCTATTTTTCTCTTGCCTGCTTGACCCATCGTTCGTACAAGTCAAAGAAATCCAAAACGTATACCAAAAACGGAACATCATGTAAAATACGTTATGGATCTGGAGCAATATCTGGTTTTTTCAGTCAAGATAATGTTAAACTTGGTGATATTGTTGTCAAGGATGTGGATTTCATTGAGGCTACCCGTGAAGGAAGTATTTCGTTTGTGCTAGCAAAGTTTGATGGATTATTTGGGCTTGGGTTTCAGGAGATCTCTGTTGAAAATGCTGTCCCAGTATGGTACGAAATGGTGCAGCAAAATCTTGTAAGTGAGAAGGTGTTCTCTTTTTGGCTCAATGGAAATCCAAAAGCTAAAAAGGGTGGTGAGCTGATTTTGGGGGGTGCTGATCCCAATCACTACAAAGGAAAACATACATATGTTCCTGTCACTCAAAAAGGTTATTGGCAGATTGAAATGGGAGATTTTTTCATTGGAGGTCTCTCAACAGGTGTCTGCGAAGGTGGCTGTGCTGCTATTGTGGATTCAGGAACATCTTTGCTTGCTGGTCCAACTACTGTTGTGACCGAAATCAACCATGCTATTGGAGCTGAAGGGGTTCTGAGCGTAGAATGTAAGGAAATCGTTACTGAATATGGAGAAATGATTTGGGATCTCTTGGTAGCAGGGGTACGACCTGGTGATGTATGTTCACAAGTTGGTTTATGTGTTGCCAAAAGAGATCAATCAAAGAGCATGGGAATTGAGATGGTGACCGAAAAGGAAGAGGGAGAGTTATCTGCTAAAGATACTGCTTTGTGCTCTTCCTGTCAAATGCTTGTGATTTGGATCCAGAATCAACTAAAGCGAAAGACAACCAAAGATAGAATATTCAACTACGTAAATCAACTGTGCGAGAGCTTGCCAAGTCCAAATGGAGAGTCCGTAGTAGACTGCAATAGCATTTATCAATTGCCAAACATTTCGTTTACCATCGGAGATAAATCTTTCGTCCTCAGTCCCGAGCAGTATATTTTGAAAACCGGAGA
TARGET=64,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03911 Ps_contig_mira-and-tgicl-ass_franssen_31460
SEQUENCE=AAGTTCATAAATTGAAAATAAATATTCGTACTATACTTTAGTCAAGGGTCCCACTCCTCTGCCATCACATTTTCTCTCTTGAAAATTCAACTCTGCTGAACTTCAACACATTATCACAAAAACATATTCTGTGTCCATGGAGAATTTGTTGGGATTGCTCAAAATTCATGTTCAAAGAGGAGTCAATCTTGCTATCAGAGATGTCAAAACCAGTGATCCTTATGTTGTCATCAAAATGGATAAACAGAAGCTGAAGACTAGGGTAGTGAAGAAGAATCTAAATCCAGAGTGGAATGAAGACTTGACTTTATCAATTTCAGATCCTAAAACTCCAATTCATCTGCATGTGTACGATAAGGACACATTCAGCTTGGATGACAAAATGGGGGACGCGGAGTTTGACATAGGTCCGTTTGTTGAAGCAATAAAGATGCGGTTGGAAGGTCTTCCGAACGAAACTATAGTTACAAAGGTGGCGCCTAGCAGACAAAATTGCTTAGCAGAAGCAAGTCACATTGTTTGGAAAGATGGTAAAGTTTTTCAAAACATGGTTCTTAGACTAAGAAATGTTGAATGTGGGGAAGTGGAACTTCAATTGCACTGGGTTGATATTCCAGGTTCAAGGGGTATGTAGGTTGATAAATACTTGTTGAATGGTTATAGATATGAGAGTTGTTGTTGCACTGTAAAACAATAACTTCTTATGCTTTAATTTGTCTTCTGTTATGATTTTTTTTACTTTTTCACTTAAGCTACAAATTGTTCAACTTGTTGAACAATGAAATATATATATTTTATGCTGGTTTGGTTTAATGAAATATATATATTTTATGCNARAAAAGACT
TARGET=784,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03912 Ps_contig_mira-and-tgicl-ass_franssen_31460
SEQUENCE=AAGTTCATAAATTGAAAATAAATATTCGTACTATACTTTAGTCAAGGGTCCCACTCCTCTGCCATCACATTTTCTCTCTTGAAAATTCAACTCTGCTGAACTTCAACACATTATCACAAAAACATATTCTGTGTCCATGGAGAATTTGTTGGGATTGCTCAAAATTCATGTTCAAAGAGGAGTCAATCTTGCTATCAGAGATGTCAAAACCAGTGATCCTTATGTTGTCATCAAAATGGATAAACAGAAGCTGAAGACTAGGGTAGTGAAGAAGAATCTAAATCCAGAGTGGAATGAAGACTTGACTTTATCAATTTCAGATCCTAAAACTCCAATTCATCTGCATGTGTACGATAAGGACACATTCAGCTTGGATGACAAAATGGGGGACGCGGAGTTTGACATAGGTCCGTTTGTTGAAGCAATAAAGATGCGGTTGGAAGGTCTTCCGAACGAAACTATAGTTACAAAGGTGGCGCCTAGCAGACAAAATTGCTTAGCAGAAGCAAGTCACATTGTTTGGAAAGATGGTAAAGTTTTTCAAAACATGGTTCTTAGACTAAGAAATGTTGAATGTGGGGAAGTGGAACTTCAATTGCACTGGGTTGATATTCCAGGTTCAAGGGGTATGTAGGTTGATAAATACTTGTTGAATGGTTATAGATATGAGAGTTGTTGTTGCACTGTAAAACAATAACTTCTTATGCTTTAATTTGTCTTCTGTTATGATTTTTTTTACTTTTTCACTTAAGCTACAAATTGTTCAACTTGTTGAACAATGAAATATATATATTTTATGCTGGTTTGGTTTAATGAAATATATATATTTTATGCNARAAAAGACT
TARGET=818,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03913 Ps_contig_mira-and-tgicl-ass_franssen_31461
SEQUENCE=GGTTTTTACTAAAGACAACCCTTCNTYTGNCTTAATTTGCTTCTTACCTCCATAACGTTAGCCAATATTCAGACTGGTTAATTGCTTACTTGAATTACTTTAGAATTGGTATTTATAACCTTATTAAACATTTCCATTTTGAAGCCTATGTTATACATATCCAATATATACATAAATTAATAGTCAAAACTGATATTTACTAAATTAACCCCTTCTCCCTCGTTGAGCAGTAGGAGGTAATTTCTCTGATGGACCAGTAGGAAGCTTTTGTCCAGTCTCTTGTTCAATCCTCCTTTTAAGCTCTCTAAGTATTCGAATTTGTCCCAGCTTCTCAGGAGACAATTTGTCCCAATAAATTCCTTGAGGCTTTGGAATGGATGTATGTGTAAAAATGACCTTGGTTGGAGTACAAATGATGTCCACTGGTACATCATGGATCAACAACTTTTCAACTGGAATATCATCTACCAACTGGCACTCATGCACAGAGGTAACAACTAGTGTTGAATCGTCGATAGCTCCCATATACCGCAGCATACCATATTCAAGTTCTGCAAATCCCTCTCCTTTTCCTAATCGAGCACCTGTAGTCGGGTCAACAGCAACAGATCCGATAACAATAAGATCTACTTTTATCTTTTCATCTAATCCGATTGGTCGTCCATATTTGGCAACCCCAACAGATGTACAGGCTTCCTTGATGGTACCCAAAGTTAACATATTGGACTCGATTACGGAGAAAAAACCCGTCCTCAGGCGTGGTTGAGGAGTTAACAACTTCTTTCCATCTGAAAGAGTGAGAAATCTGACTTGTTTCTGAGGAGAATCTGGATTAACCTTCACACATTGTGCACCGCGGAACACATCTAGCTCCCTCATCTTTTCAGCAGCAAGAGAAGCACCAACGAAATTGGGGATTCTATGATGAACAGGCCTCGGACTTTGTGCAAAATTTTTATCTTCCATCAAATCCCAAATTCTCTTTCGGATTATCCATTTCCAAGCTTTGGGATCATCTCGGAGAGAAGAATTCTCCTCTTGAACTGCCATTGCTTGGCGAGCTTCGGCGTCGAGGGTTAACCGCTCGGTCTCGTAAGCAGCCTCGTTGAAGGGAGTGTTGTTGTTGCTGCTGTTGGTGGCTTCCAGGTTGAAGAAGCTGCAAGTGTGGGGTTTGATGGAGAGAGAGTGAGAGTGGGTTTTGTTCCAAAGAGAAGAGAGAGTTTTGATGTAGTGGTTGTTGTTGTTGGTTTTGGTGAATGTTAATGGATAGCATAACCGAAATGCTTGTGTATCCATTTTAAGAGAGTTTTCGGTTTCAATTTCTGGTTAGTTATACAAGTACATTCT
TARGET=1234,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03914 Ps_contig_mira-and-tgicl-ass_franssen_31473
SEQUENCE=CAAACAACAATTGTAGCATCAAAACAGTTACTAATGGAAGCTCCAAGATTTGTCTTCTTGTCTTTGCTTCTTCTTCTTCTCACAGCTTCAACAACCTCAGCTCAACTCACTCGTGGCTTCTATAACAATGTATGTCCCAATGTTGAACAAGTGGTTCGTGCTGCCGTCACTCAGAAGTTTCAACAAACTATTTGTGACTGCTCCTGCCACTCTTAGACTCTTCTTCCATGATTGCTTTGTCAGGGGTTGTGATGCTTCGGTTTTGATTCAATCTCCAAATAATAAGGCCGAGAAGGATCATCCTGATGATGTGTCGCTAGCCGGGGACGGCTTTGACACGGTGATTAAGGCCAAGGCGGCTGTTGACAAGGATCCTAAGTGTCGAAACAAAGTCTCTTGTGCAGATATTCTCGCTCTCGCCACTAGAGATGTCGTCAATTTGGCCGGGGGTCCGTTTTATAATGTCGATTTAGGAAGGCGCGATGGAAGAATATCTACTTTGGCTGGTGTTCAACACAATCTTCCTGCCCCTAGTTTCAAATTGGATCAACTCAACACCATGTTTAATCGACACGGTCTCTCCCAAACCGATATGATTGCATTATCAGGTGCACATACAATTGGATTCTCTCACTGCAACCGATTCTCAAATCGAATCTACAGATTCAGCNGAACAACGAGAATCGATCCATCATTGAATTTACAATATGCTTTACAGCTTAGGCAAATGTGTCCATTAAAAGTTGATCCTAGAATTGCCATTAACATGGATCCTGTCTCACCTAGAAAGTTTGACAATCAATATTTCATAAATCTACAACAAGGAAAAGGACTATTCACTTCTGATCAAGTGTTGTTTACAGATTCAAGGTCAAAGGCTACAGTTAACTTATTTGCATCCAATGAAAAAGTCTTTTNAAACTGTCTTTTTATTGATGTCTTAATTAACGTAAGGTTAGGGGTAAAGGTTTA
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4567
PRIMER_LEFT_PENALTY=0.401769
PRIMER_RIGHT_PENALTY=0.054900
PRIMER_LEFT_SEQUENCE=AATGGAAGCTCCAAGATTTGTC
PRIMER_RIGHT_SEQUENCE=GGAGATTGAATCAAAACCGAAG
PRIMER_LEFT=32,22
PRIMER_RIGHT=275,22
PRIMER_LEFT_TM=59.598
PRIMER_RIGHT_TM=59.945
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.4575
PRIMER_LEFT_1_PENALTY=0.401769
PRIMER_RIGHT_1_PENALTY=0.055692
PRIMER_LEFT_1_SEQUENCE=AATGGAAGCTCCAAGATTTGTC
PRIMER_RIGHT_1_SEQUENCE=TCTCGGCCTTATTATTTGGAGA
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=292,22
PRIMER_LEFT_1_TM=59.598
PRIMER_RIGHT_1_TM=60.056
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.4659
PRIMER_LEFT_2_PENALTY=0.401769
PRIMER_RIGHT_2_PENALTY=0.064105
PRIMER_LEFT_2_SEQUENCE=AATGGAAGCTCCAAGATTTGTC
PRIMER_RIGHT_2_SEQUENCE=CACGAACCACTTGTTCAACATT
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=159,22
PRIMER_LEFT_2_TM=59.598
PRIMER_RIGHT_2_TM=59.936
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=128
PRIMER_PAIR_PENALTY_3=0.4831
PRIMER_LEFT_3_PENALTY=0.401769
PRIMER_RIGHT_3_PENALTY=0.081328
PRIMER_LEFT_3_SEQUENCE=AATGGAAGCTCCAAGATTTGTC
PRIMER_RIGHT_3_SEQUENCE=GTCTAAGAGTGGCAGGAGCAGT
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=217,22
PRIMER_LEFT_3_TM=59.598
PRIMER_RIGHT_3_TM=60.081
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=0.4836
PRIMER_LEFT_4_PENALTY=0.401769
PRIMER_RIGHT_4_PENALTY=0.081801
PRIMER_LEFT_4_SEQUENCE=AATGGAAGCTCCAAGATTTGTC
PRIMER_RIGHT_4_SEQUENCE=GAGATTGAATCAAAACCGAAGC
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=59.598
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_03915 Ps_contig_mira-and-tgicl-ass_franssen_31477
SEQUENCE=AATTTTATTTTATTTTTGCAATAATTTAGTTGGATAATGCAATGTNGNNNAANGGAAGTGGAAATAATTCCGACTAACAAACAAGTTGACAGCCAACATAGCTCGCAGAAGATTCAAGAGCGAATGAACAACAAGCAAGAACAAAGAAAAAGAAAAATTAAAATAAAATACATCGCATCCACTCATTCAATCACTCACACACCAGAAAGATTCCTCCTGTTCCCGCTTTCTCTCTCGATCTGCTACTGCTAGGACTCGGAGTAGTATGGATTGCACCGCCGGTACCTGTTTGTTTCCGTTACATCGCTGCAAAACTATTCACCTCGTGAGGCATGCGCAGGGGATCCACAATGTGGAAGGAGATAAGAACTACAAAGCGTACTTGAATCCTGAATATTTCGATGCTCATCTCACTCCTCTCGGATGGGAGCAGGTTGATAATTTGCGTAAGCATGTTCACTCCTCTGGTTTGATAAACAAGATTGATCTCGTTATCGCTTCTCCTTTGATGAGGACTCTGCAAACAGCTGTTGGGGTATTTGGTGGGGAGGGATACAGTGGTGATAAAACAGATGTGGTGCCTCTTATGGTGGCAAATGCCGGAAATAGCTTTCATGCTGCAATTTCAAGTCTTAATTGCCCACCAATTGTTGCTGGCGAACTTTGTCGTGAACATTTGGGAGTTCATCCCTGTGACAGAAGAAGAAGTGTTAGCGAGTATCAGTTTCTTTTTCCCGCTGTTGATTTTTCACTGATAGACAGTGATGAGGATGTTTGGTGGAAGGATGATGTGAGAGAGACAAAGGAGGAACTTGCAGCTAGGGGGCTGAAGTTCATGAACTGGTTGTGGACACGAAAAGAGAAGGAGATAGCGATTGTGACTCACAGTGGATTCTTGTTTCACACTCTAACTGCATTTGGAAATGATTGCCACCCCTCAGTGAAGAAAGAAATATCCAAGCACTTTGCGAACTGTGAGCTTCGCTCCATGGTCATTGTGGATAGAAGTATGTTAGGGTCGGAAGAATCAACAACTAACTATCCCGGCAAGATACCTTCAGGGCTGGACAAACCTAGTGATGCTGTGGATGAGATCGTTGAGAAGCAGGGCGTTTAGTTTTGCTACATGTATGTGTTTAATTCCATTTAAGCATTGAACTGCTGGGCATATAATCATTACTATTTGAAGATGATGACTCATACAAAATCTAACATGATTGTTTCAGACTTTACTTGGTGCTAAAGACCTTGCAAGACTARCTATTTAGTGTCTTAG
TARGET=2,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03916 Ps_contig_mira-and-tgicl-ass_franssen_31480
SEQUENCE=GTTTGGGTTGGTAGTCAACTACCACCTATCAGTTTCTATTGTAGAAAATAAAATAGACTGGTAAGTGCCGCTGCAGCCCTTACTGGCTGGTCTTGCTCCTAAGACAAAGGTTCTTAGGGATGGTAAATGGTCTGAGCAGGAAGCTGCAATTTTAGTCCCTGGAGACATCATCAGCATCAAACTAGGTGATATTGTTCCAGCTGATGCTCGTCTTCTTGAAGGTGATCCTTTAAAGATTGATCAGTCTGCTTTGACTGGAGAATCACTTCCTGTGACTAGAAGTCCTGGGCAAGAAGTTTTCTCTGGCTCAACTTGTAAACAAGGTGAAATTGAAGCTGTTGTCATTGCAACTGGTGTCCACACATTTTTCGGGAAAGCAGCTCACCTTGTGGACAGTACAAACCAAGTTGGACATTTCCAGAAGGTGCTTACCGCAATTGGAAACTTCTGTATCTGTTCCATTGCAGTTGGTATGTTGGCTGAGATCATAGTCATGTACCCAATTCAGCACCGCAAGTACAGAGACGGAATTGACAACCTTTTGGTCCTTTTGATCGGAGGAATTCCTATTGCTATGCCCACTGTGTTGTCTGTAACTATGGCCATTGGTTCACACAGGCTCTCTCAGCAAGGAGCTATTACCAAGCGTATGACTGCTATTGAAGAAATGGCTGGTATGGATGTACTTTGCAGTGACAAGACTGGTACACTTACACTTAACAAGCTGAGTGTTGACAAGAACTTGATTGAGGTCTTTGAAAAGGGTGTGGACAAGGAACATGTTATGCTTCTTGCTGCAAGGGCTTCCAGGATTGAAAACCAGGATGCCATAGATGCCGCCATTGTTGGAACTCTAGCTGATCCAAAAGAGGCAAGGGCTGGAGTAAGAGAGATTCATTTCTTACCATTCAATCCCGTGGATAAGAGAACTGCCTTGACTTACATTGATAGCAATGGAAATTGGCACCGTGCAAGCAAAGGTGCTCCAGAGCAGATCATGAACCTGTGTAACCTTAGGGAAGATGCAAAGCGGAACATCCATGCTATTATTGACAAGTTTGCAGAGAGAGGTCTTCGTTCTCTTGCTGTTTCTAGACAGGAGGTTCCTGAGAAAACTAAAGAAAGTGCTGGTGGTCCTTGGCAGTTTGTTGGTTTGTTGTCACTGTTTGACCCACCTAGGCATGACAGTGCTGAAACTATTCGGAGAGCTCTTCATCTTGGTGTCAATGTTAAGATGATTACTGGTGATCAACTTGCCATAGCAAAGGAGACTGGGAGGAGACTTGGGATGGGAACTAATATGTACCCATCTGCTACCTTGCTTGGACAAGACAAAGATGCGAGTATTGCTGCACTTCCAGTTGAAGAGCTGATTGAGAAGGCAGATGGATTTGCTGGGGTATTTCCAGAGCACAAATATGAAATTGTAAAGAAGTTGCAAGAAAGGAAACACATTTGTGGAATGACTGGAGATGGTGTCAACGATGCTCCAGCTTTGAAGAAGGCCGATATTGGAATCGCTGTTGCCGATGCTACGGATGCCGCAAGAGGTGCTTCTGATATTGTCCTCACAGAACCTGGATTGAGTGTTATCATCAGTGCAGTCTTAACTAGTAGGGCTATTTTCCAAAGGATGAAAAACTACACGATCTATGCAGTATCTATCACTATCCGTATAGTGTTTGGCTTCATGTTCATTGCACTGATATGGAAATTTGACTTCTCACCTTTCATGGTCTTGATTATTGCCATTCTTAATGATGGAACAATCATGACAATTTCAAAAGATAGGGTGGTTCCATCTCCCTTACCCGATAGCTGGAAATTGAATGAAATATTTGCTACTGGGATTGTTCTTGGAGGTTATTTAGCTCTGATGACCGTAATATTCTTCTGGGCAATTAAAGAAAATAAATTCTTCCCTGACAAATTTGGAGTTAGGCACCTGAATCATGATGAAATGATGTCTGCTTTGTATCTTCAAGTCAGTATAGTTAGCCAGGCTTTGATATTTGTGACCCGTTCTCGTGGTTGGTCGTTTCTTGAACGCCCTGGAGCCCTATTAGTCATTGCTTTCCTCATTGCTCAGCTGATTGCTACATTAATAGCAGTATATGCCAACTGGGGATTTGCAAAGGTACAGGGAATTGGTTGGGGTTGGGCAGGAGTAATCTGGCTCTACAGTATTGTCTTCTATATTCCACTCGACGTGATGAAATTTGCCATCCGCTACATATTGAGTGGCAAGGCTTGGAACAATCTCCTAGACAACAAGACTGCCTTCACCACCAAGAAAGACTACGGAAAAGAGGAGAGGGAAGCTCAATGGGCACACGCTCAGAGGACTCTACATGGACTTCAACCACCAGAGAGTTCTGGTATTTTCAACGAGAAGAGCAGTTACAGGGAACTCTCTGAGATTGCCGAGCAGGCCAAGAGAAGAGCCGAAGTTGCAAGGCTTCGCGAGCTGCACACACTGAAAGGGCATGTTGAGTCTGTGGTGAAGCTAAAAGGTTTGGACATTGATACCATCCAGCAGCACTACACTGTGTGAGAAGCTCTGTAAAATTGTAGATACGAGATTGGCCAAAGTTGAACAATGTGATATGTGAATGAAAGCAATAAAAGTCAAAGAGAAGGCCTAACTACTTTAGTGCTTACCAGGAATGGAGAGAGGAGAGGAGAGTGTGAACCTTTTGATATTAGCTTAGTGTTTTTTCCTGTGTACTTTTAAGTTCTGCCTAAACGGATAGTCTTTGCCTTGTATTTGCTTCCATGATAAGAGCAAAAACTAGTTTCAATTTCGTTTCCCTTAAGCAATTAGTTTCAGTTTCCGTCCTTGACTTTATTATCAGTTTCATATGTATGGTTTTGGGTGTTTTCTCATTTTAATTGTATGCCACATGGCAGTCATCAGATAATGTCGATATTGGGATTTTTTTAAGGTGGGTGAATAAAGTGCTTTTGTATAAGGTTTTAAAAAAAAACC
TARGET=2672,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3746
PRIMER_LEFT_PENALTY=0.035021
PRIMER_RIGHT_PENALTY=0.339582
PRIMER_LEFT_SEQUENCE=GTGGTGAAGCTAAAAGGTTTGG
PRIMER_RIGHT_SEQUENCE=ATCATGGAAGCAAATACAAGGC
PRIMER_LEFT=2497,22
PRIMER_RIGHT=2779,22
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=60.340
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.5012
PRIMER_LEFT_1_PENALTY=0.161579
PRIMER_RIGHT_1_PENALTY=0.339582
PRIMER_LEFT_1_SEQUENCE=GATACCATCCAGCAGCACTACA
PRIMER_RIGHT_1_SEQUENCE=ATCATGGAAGCAAATACAAGGC
PRIMER_LEFT_1=2524,22
PRIMER_RIGHT_1=2779,22
PRIMER_LEFT_1_TM=60.162
PRIMER_RIGHT_1_TM=60.340
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.5012
PRIMER_LEFT_2_PENALTY=0.161579
PRIMER_RIGHT_2_PENALTY=0.339582
PRIMER_LEFT_2_SEQUENCE=TGATACCATCCAGCAGCACTAC
PRIMER_RIGHT_2_SEQUENCE=ATCATGGAAGCAAATACAAGGC
PRIMER_LEFT_2=2523,22
PRIMER_RIGHT_2=2779,22
PRIMER_LEFT_2_TM=60.162
PRIMER_RIGHT_2_TM=60.340
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.1000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.5141
PRIMER_LEFT_3_PENALTY=0.174509
PRIMER_RIGHT_3_PENALTY=0.339582
PRIMER_LEFT_3_SEQUENCE=TACCATCCAGCAGCACTACACT
PRIMER_RIGHT_3_SEQUENCE=ATCATGGAAGCAAATACAAGGC
PRIMER_LEFT_3=2526,22
PRIMER_RIGHT_3=2779,22
PRIMER_LEFT_3_TM=59.825
PRIMER_RIGHT_3_TM=60.340
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.6305
PRIMER_LEFT_4_PENALTY=0.035021
PRIMER_RIGHT_4_PENALTY=0.595491
PRIMER_LEFT_4_SEQUENCE=GTGGTGAAGCTAAAAGGTTTGG
PRIMER_RIGHT_4_SEQUENCE=GGAAGCAAATACAAGGCAAAGA
PRIMER_LEFT_4=2497,22
PRIMER_RIGHT_4=2774,22
PRIMER_LEFT_4_TM=60.035
PRIMER_RIGHT_4_TM=60.595
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_03917 Ps_contig_mira-and-tgicl-ass_franssen_31483
SEQUENCE=AAAGTCCTCAACAAGGAAAGGAAATGAGGTTCTTTTGCTTGTCGATTGAGAAGAAAAAAAAACAACTGGAAATCTATATACTGTTTAACTTTCCTTAACATGTACAAAAATAAGCAACATGCATTTGAAAAAGCTGATAGAAGTGAAAGCATTTACATTTACATTGAGGGTAGCAAAGTTATTCCTCAAAAAAGACACAAAAAGAAGTAGAAAAAAAAAGTCAAACAGATCCAAAAAGTGAAACTCAACCTCAAACCACATAGTAGCTGAAAAGAGAGAGAAGATTAAATGAACCCAAAATAAAAAACATACAAACAAAGAGGGTGTTATTTTGTTTATTTTTTTTAGTATGCATAAGCTTTAACAACTATCCACACAGAAATCTAGATCTACCATAGATATCTTGTTCTCTTGGAATTGGAACCAGGACTAATCTGTGGAACTGTGAAAATGTTACCATCATCCCTCAACCTTGATCTGTTGATGCTGCTTTTGTTGTTGCTGTTTTTCTTGGTCTCACTCTGACCCATCATCATTATCTGTGAAGAAGAATATAGAGAAGGTGGTGGTGCTTGAGGAACCCAGATCCCAATATTACTATTAGGAAAAAATGAATCAAGATTTTCTTCAAACAATTCATCACCCTTAGCGAAAGAAAAGGGAACAGAAGAAGAATCTAGAAAATCATCAACTTGCCAACCAGGTAGAGTCTCAATCAAGTACTTAGAAATACTTGAACCACCACTTCCTTCTTCTTCTTCTTCCATGGCTACGTTTTTGAGAGTTGAAGTTTGTGATATAGTACTTGATTTTTCAATCAAAGAAGAAGGAACAAGATTCGACTTAGAATTAGTAGTTGTTGTTGTTGATGACGAAGATGGAGAGTTAGTACTAGTAGTTGAAAGCTTAACACCAGTGAGAAGAAACCTATCATGTTTCTGAGTAAGTTCGTTAACAGAATGAATAGATGAATCACAATCTTTACACAGAAGCGCTCTATCTTGTTTACACAACACAAAAGCTCTTCTCTCCTGGCAGATATCACAGAGAGGGTGTTGTTTAGGAGAAGGACTGTGAAGAGAGAGACGGTGGTGTTTGGAGGCTAGTTTGTTGGCGTGGTGGACACGGTGGTCGCAGTCGTTGCATAAGGCGGCTTCATCGGCGGTGCAGAATAATGAAGCTTCGTTTTTGTTGCAAACGTCGCACTGGATCTTCATTTTTTTTTGGTTTGAGAAAATGGAAATGATTGAGTTAGATAAATGAAGAAATGAAGAAATTATTTAATGGAAAAGATGGCTCCATGATCAACGAGGGAGTAGTTTATGTGAAGAAATAAAAATAGGTAACCCCCATTATCGCTTAGGCATTATAATATAT
TARGET=749,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1758
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.080323
PRIMER_LEFT_SEQUENCE=ATTCATCACCCTTAGCGAAAGA
PRIMER_RIGHT_SEQUENCE=TCAACTCTCAAAAACGTAGCCA
PRIMER_LEFT=634,22
PRIMER_RIGHT=787,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.920
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.1877
PRIMER_LEFT_1_PENALTY=0.107351
PRIMER_RIGHT_1_PENALTY=0.080323
PRIMER_LEFT_1_SEQUENCE=CTTAGCGAAAGAAAAGGGAACA
PRIMER_RIGHT_1_SEQUENCE=TCAACTCTCAAAAACGTAGCCA
PRIMER_LEFT_1=644,22
PRIMER_RIGHT_1=787,22
PRIMER_LEFT_1_TM=59.893
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=0.1877
PRIMER_LEFT_2_PENALTY=0.107351
PRIMER_RIGHT_2_PENALTY=0.080323
PRIMER_LEFT_2_SEQUENCE=TTAGCGAAAGAAAAGGGAACAG
PRIMER_RIGHT_2_SEQUENCE=TCAACTCTCAAAAACGTAGCCA
PRIMER_LEFT_2=645,22
PRIMER_RIGHT_2=787,22
PRIMER_LEFT_2_TM=59.893
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=143
PRIMER_PAIR_PENALTY_3=0.2001
PRIMER_LEFT_3_PENALTY=0.119809
PRIMER_RIGHT_3_PENALTY=0.080323
PRIMER_LEFT_3_SEQUENCE=GTCTCACTCTGACCCATCATCA
PRIMER_RIGHT_3_SEQUENCE=TCAACTCTCAAAAACGTAGCCA
PRIMER_LEFT_3=513,22
PRIMER_RIGHT_3=787,22
PRIMER_LEFT_3_TM=60.120
PRIMER_RIGHT_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=0.2149
PRIMER_LEFT_4_PENALTY=0.134571
PRIMER_RIGHT_4_PENALTY=0.080323
PRIMER_LEFT_4_SEQUENCE=CGAAAGAAAAGGGAACAGAAGA
PRIMER_RIGHT_4_SEQUENCE=TCAACTCTCAAAAACGTAGCCA
PRIMER_LEFT_4=649,22
PRIMER_RIGHT_4=787,22
PRIMER_LEFT_4_TM=59.865
PRIMER_RIGHT_4_TM=59.920
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_03918 Ps_contig_mira-and-tgicl-ass_franssen_31483
SEQUENCE=AAAGTCCTCAACAAGGAAAGGAAATGAGGTTCTTTTGCTTGTCGATTGAGAAGAAAAAAAAACAACTGGAAATCTATATACTGTTTAACTTTCCTTAACATGTACAAAAATAAGCAACATGCATTTGAAAAAGCTGATAGAAGTGAAAGCATTTACATTTACATTGAGGGTAGCAAAGTTATTCCTCAAAAAAGACACAAAAAGAAGTAGAAAAAAAAAGTCAAACAGATCCAAAAAGTGAAACTCAACCTCAAACCACATAGTAGCTGAAAAGAGAGAGAAGATTAAATGAACCCAAAATAAAAAACATACAAACAAAGAGGGTGTTATTTTGTTTATTTTTTTTAGTATGCATAAGCTTTAACAACTATCCACACAGAAATCTAGATCTACCATAGATATCTTGTTCTCTTGGAATTGGAACCAGGACTAATCTGTGGAACTGTGAAAATGTTACCATCATCCCTCAACCTTGATCTGTTGATGCTGCTTTTGTTGTTGCTGTTTTTCTTGGTCTCACTCTGACCCATCATCATTATCTGTGAAGAAGAATATAGAGAAGGTGGTGGTGCTTGAGGAACCCAGATCCCAATATTACTATTAGGAAAAAATGAATCAAGATTTTCTTCAAACAATTCATCACCCTTAGCGAAAGAAAAGGGAACAGAAGAAGAATCTAGAAAATCATCAACTTGCCAACCAGGTAGAGTCTCAATCAAGTACTTAGAAATACTTGAACCACCACTTCCTTCTTCTTCTTCTTCCATGGCTACGTTTTTGAGAGTTGAAGTTTGTGATATAGTACTTGATTTTTCAATCAAAGAAGAAGGAACAAGATTCGACTTAGAATTAGTAGTTGTTGTTGTTGATGACGAAGATGGAGAGTTAGTACTAGTAGTTGAAAGCTTAACACCAGTGAGAAGAAACCTATCATGTTTCTGAGTAAGTTCGTTAACAGAATGAATAGATGAATCACAATCTTTACACAGAAGCGCTCTATCTTGTTTACACAACACAAAAGCTCTTCTCTCCTGGCAGATATCACAGAGAGGGTGTTGTTTAGGAGAAGGACTGTGAAGAGAGAGACGGTGGTGTTTGGAGGCTAGTTTGTTGGCGTGGTGGACACGGTGGTCGCAGTCGTTGCATAAGGCGGCTTCATCGGCGGTGCAGAATAATGAAGCTTCGTTTTTGTTGCAAACGTCGCACTGGATCTTCATTTTTTTTTGGTTTGAGAAAATGGAAATGATTGAGTTAGATAAATGAAGAAATGAAGAAATTATTTAATGGAAAAGATGGCTCCATGATCAACGAGGGAGTAGTTTATGTGAAGAAATAAAAATAGGTAACCCCCATTATCGCTTAGGCATTATAATATAT
TARGET=856,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1327
PRIMER_LEFT_PENALTY=0.080323
PRIMER_RIGHT_PENALTY=0.052366
PRIMER_LEFT_SEQUENCE=TGGCTACGTTTTTGAGAGTTGA
PRIMER_RIGHT_SEQUENCE=GCGCTTCTGTGTAAAGATTGTG
PRIMER_LEFT=766,22
PRIMER_RIGHT=994,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.1815
PRIMER_LEFT_1_PENALTY=0.129103
PRIMER_RIGHT_1_PENALTY=0.052366
PRIMER_LEFT_1_SEQUENCE=TCTTCTTCCATGGCTACGTTTT
PRIMER_RIGHT_1_SEQUENCE=GCGCTTCTGTGTAAAGATTGTG
PRIMER_LEFT_1=756,22
PRIMER_RIGHT_1=994,22
PRIMER_LEFT_1_TM=60.129
PRIMER_RIGHT_1_TM=59.948
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.1815
PRIMER_LEFT_2_PENALTY=0.129103
PRIMER_RIGHT_2_PENALTY=0.052366
PRIMER_LEFT_2_SEQUENCE=TTCTTCTTCCATGGCTACGTTT
PRIMER_RIGHT_2_SEQUENCE=GCGCTTCTGTGTAAAGATTGTG
PRIMER_LEFT_2=755,22
PRIMER_RIGHT_2=994,22
PRIMER_LEFT_2_TM=60.129
PRIMER_RIGHT_2_TM=59.948
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=0.1981
PRIMER_LEFT_3_PENALTY=0.145769
PRIMER_RIGHT_3_PENALTY=0.052366
PRIMER_LEFT_3_SEQUENCE=TGAACCACCACTTCCTTCTTCT
PRIMER_RIGHT_3_SEQUENCE=GCGCTTCTGTGTAAAGATTGTG
PRIMER_LEFT_3=734,22
PRIMER_RIGHT_3=994,22
PRIMER_LEFT_3_TM=60.146
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.2440
PRIMER_LEFT_4_PENALTY=0.080323
PRIMER_RIGHT_4_PENALTY=0.163658
PRIMER_LEFT_4_SEQUENCE=TGGCTACGTTTTTGAGAGTTGA
PRIMER_RIGHT_4_SEQUENCE=TGTGATATCTGCCAGGAGAGAA
PRIMER_LEFT_4=766,22
PRIMER_RIGHT_4=1045,22
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=59.836
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_03919 Ps_contig_mira-and-tgicl-ass_franssen_31489
SEQUENCE=GTCGGGGATTAAACTAGTCTATTACATTGCATAGAAGAGAAGACCAACGCGGAGGGATTTTGGCCTCCACCGACATCTCTTCTCAATTCTCAAATTCACATCAATTATATTTCTCATCATCATCATCATCATCATCATCATCATCATCATCATCATCAATGGCTGATGATAACAAGAAACTCAATCACGACGAGGAGGCAAATCCTCTTCTCCACCACCACCATCACCAACAACAACCACCACCTAAAGCAGCTGAACAACCAACACAGGAGGAACTTTTTCATCTATGGACCGCTGATGGTGTTCCCATCGCCCATGGAAGCGTAATGGGCCAGCCTATTCCCCGTTCTCCTTGGAATTCCAGCGTCTGCGCTTGTCTCGGTCAAAGCGATCACTTTTGCAGCAGCGATCTCGAAGTTTGTCTTCTTGGGAGTGTGGCTCCTTGTGTGCTGTATGGAAGCAATGTTGAGAGACTTGGGTCCAATAATTCCGGGACATTTGCCAATCATTGTTTGCATTATTCTGGCCTCTATGTAATTGGGAATTCCTGCTGTGGTTGGAATTGTCTTGCGCCGTGGTTTTCATATCCTAGCCGAACTGCAATTCGTCGCAGGTTCAATTTGGAGGGAAGCTGTGAGGCACTTAATAGGTCATGTGGGTGCTGCGGAAGCTTTTTGGAAGATGAGGCGCAGCGTGAACAGTGCGAATTGGCATGTGACTTTGCAACCCATTTCTTTTGTCATGCCTGTGCTCTTTGTCAAGAAGGTCGTGAGCTCCGCCGTAGAGTGCCTCATCCTGGCTTCAATGCGCAACAAATATTGGTTATGATCCCACCTGCAGAGCAGGCAATGGGTCGTGGGGCGTGAAAAACATTCTTCCTCATCTTGGAAGTGCTATTCTGGTGTATGCTATTACTGTTGCTTTGATATGATTTGACACGTCGAACTGCTGCCTCCCTTTTCTCCAGATTTTGCTGAAGAGTATCATGGTTGTTTGTTTGAGTTTGGTATAAATAAAGAACATACAGCACCATTATATTTTCAGTTTGTATCCTCTACTATTCTGTAGTATATATGTATTTTGTTGCAATATGAATTTATATGTATTATTGAGGGCATGGTTCCTGTATGGTTATGAGTGGTTGTAGCCTGTAATTCAGTGAATGCATATTATGTTTTAAATATAAAAAAATAGTTAATATCATCTAATTATTTTTTAATTAATAAATAAAAAGAAAAAATA
TARGET=114,45
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4870
PRIMER_LEFT_PENALTY=0.400659
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=GCATAGAAGAGAAGACCAACGC
PRIMER_RIGHT_SEQUENCE=CTCGTCGTGATTGAGTTTCTTG
PRIMER_LEFT=28,22
PRIMER_RIGHT=193,22
PRIMER_LEFT_TM=60.401
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.5696
PRIMER_LEFT_1_PENALTY=0.483253
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=TGCATAGAAGAGAAGACCAACG
PRIMER_RIGHT_1_SEQUENCE=CTCGTCGTGATTGAGTTTCTTG
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=193,22
PRIMER_LEFT_1_TM=59.517
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.6205
PRIMER_LEFT_2_PENALTY=0.400659
PRIMER_RIGHT_2_PENALTY=0.219884
PRIMER_LEFT_2_SEQUENCE=GCATAGAAGAGAAGACCAACGC
PRIMER_RIGHT_2_SEQUENCE=TCGTCGTGATTGAGTTTCTTGT
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=192,22
PRIMER_LEFT_2_TM=60.401
PRIMER_RIGHT_2_TM=59.780
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.7031
PRIMER_LEFT_3_PENALTY=0.483253
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=TGCATAGAAGAGAAGACCAACG
PRIMER_RIGHT_3_SEQUENCE=TCGTCGTGATTGAGTTTCTTGT
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=192,22
PRIMER_LEFT_3_TM=59.517
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.8944
PRIMER_LEFT_4_PENALTY=0.400659
PRIMER_RIGHT_4_PENALTY=0.493727
PRIMER_LEFT_4_SEQUENCE=GCATAGAAGAGAAGACCAACGC
PRIMER_RIGHT_4_SEQUENCE=GCGGTCCATAGATGAAAAAGTT
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=60.401
PRIMER_RIGHT_4_TM=59.506
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_03920 Ps_contig_mira-and-tgicl-ass_franssen_31489
SEQUENCE=GTCGGGGATTAAACTAGTCTATTACATTGCATAGAAGAGAAGACCAACGCGGAGGGATTTTGGCCTCCACCGACATCTCTTCTCAATTCTCAAATTCACATCAATTATATTTCTCATCATCATCATCATCATCATCATCATCATCATCATCATCATCAATGGCTGATGATAACAAGAAACTCAATCACGACGAGGAGGCAAATCCTCTTCTCCACCACCACCATCACCAACAACAACCACCACCTAAAGCAGCTGAACAACCAACACAGGAGGAACTTTTTCATCTATGGACCGCTGATGGTGTTCCCATCGCCCATGGAAGCGTAATGGGCCAGCCTATTCCCCGTTCTCCTTGGAATTCCAGCGTCTGCGCTTGTCTCGGTCAAAGCGATCACTTTTGCAGCAGCGATCTCGAAGTTTGTCTTCTTGGGAGTGTGGCTCCTTGTGTGCTGTATGGAAGCAATGTTGAGAGACTTGGGTCCAATAATTCCGGGACATTTGCCAATCATTGTTTGCATTATTCTGGCCTCTATGTAATTGGGAATTCCTGCTGTGGTTGGAATTGTCTTGCGCCGTGGTTTTCATATCCTAGCCGAACTGCAATTCGTCGCAGGTTCAATTTGGAGGGAAGCTGTGAGGCACTTAATAGGTCATGTGGGTGCTGCGGAAGCTTTTTGGAAGATGAGGCGCAGCGTGAACAGTGCGAATTGGCATGTGACTTTGCAACCCATTTCTTTTGTCATGCCTGTGCTCTTTGTCAAGAAGGTCGTGAGCTCCGCCGTAGAGTGCCTCATCCTGGCTTCAATGCGCAACAAATATTGGTTATGATCCCACCTGCAGAGCAGGCAATGGGTCGTGGGGCGTGAAAAACATTCTTCCTCATCTTGGAAGTGCTATTCTGGTGTATGCTATTACTGTTGCTTTGATATGATTTGACACGTCGAACTGCTGCCTCCCTTTTCTCCAGATTTTGCTGAAGAGTATCATGGTTGTTTGTTTGAGTTTGGTATAAATAAAGAACATACAGCACCATTATATTTTCAGTTTGTATCCTCTACTATTCTGTAGTATATATGTATTTTGTTGCAATATGAATTTATATGTATTATTGAGGGCATGGTTCCTGTATGGTTATGAGTGGTTGTAGCCTGTAATTCAGTGAATGCATATTATGTTTTAAATATAAAAAAATAGTTAATATCATCTAATTATTTTTTAATTAATAAATAAAAAGAAAAAATA
TARGET=212,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1119
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.025566
PRIMER_LEFT_SEQUENCE=CAAGAAACTCAATCACGACGAG
PRIMER_RIGHT_SEQUENCE=GAAGACAAACTTCGAGATCGCT
PRIMER_LEFT=172,22
PRIMER_RIGHT=425,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.1528
PRIMER_LEFT_1_PENALTY=0.086339
PRIMER_RIGHT_1_PENALTY=0.066501
PRIMER_LEFT_1_SEQUENCE=CAAGAAACTCAATCACGACGAG
PRIMER_RIGHT_1_SEQUENCE=ACATTGCTTCCATACAGCACAC
PRIMER_LEFT_1=172,22
PRIMER_RIGHT_1=465,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=60.067
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=0.2320
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.012153
PRIMER_LEFT_2_SEQUENCE=ACAAGAAACTCAATCACGACGA
PRIMER_RIGHT_2_SEQUENCE=GAAAAAGTTCCTCCTGTGTTGG
PRIMER_LEFT_2=171,22
PRIMER_RIGHT_2=281,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=0.2454
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=0.025566
PRIMER_LEFT_3_SEQUENCE=ACAAGAAACTCAATCACGACGA
PRIMER_RIGHT_3_SEQUENCE=GAAGACAAACTTCGAGATCGCT
PRIMER_LEFT_3=171,22
PRIMER_RIGHT_3=425,22
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=60.026
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.2864
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=0.066501
PRIMER_LEFT_4_SEQUENCE=ACAAGAAACTCAATCACGACGA
PRIMER_RIGHT_4_SEQUENCE=ACATTGCTTCCATACAGCACAC
PRIMER_LEFT_4=171,22
PRIMER_RIGHT_4=465,22
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=60.067
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_03921 Ps_contig_mira-and-tgicl-ass_franssen_31491
SEQUENCE=GAATTCTAACNATTGAGAATAAATGTGATGAAATGAGTGTTTCTCTTTGCAATTAGCAATTGGAAAATATCAGTTCAGTATTTTTTTTTGGTTACAAAGTTGGGTACAATTACAAAAGATCACATAACATGGTTTTTCTGTTGAAGGATGCAACATTCAATTCCACCAGGCAATTTCCTAGGCTAACTTGCTCCACTTCTTGACCTACTGTTCATATCTGTAACCGGTATAGTGAGTGGCGTAACACAAAATGGAACTTACTACTCTACACTAGTTCATCAATCTTCACATGCTGCATCCATATCCAATGTTGTACAATCTCAGCCCAACTAGCATTGCCTTGATGGTATGACGCAAGCGGCAATCAAATGATAAATATTTTCGTCGAAATCACATGCAGCCATGTATGTATACCAAGAACCCAGATCTGATCTACTATCAAGCATTACCTACCAACTCGTGTAGTTATATATCGTCCCTTTTCTAGGTCATAAATTGTCTTCAACTGAGGTTTCCTCCACCTAGATATAGACCATGCTGTAAACAACAGTATTATAAAAATTCCAACCAGTGTGAGGAGAGTGGGTGATTCATTGCTGAAAATGGAGGCAATCCAGTTATGGTTATGTGGATCTGAATCCGCTGTAGTTTGCAGAATAAAAGCTCCTAAAGCCCAGTCGAGCGGAATGTTTCTCACCTGATTAGCAACTTTGATCCTATCATCATCCATACCAATCCCAAGACTGTCATGAAGCAGAGCAACAATGTAAGCAGAAGAGAAGCAATGTCGAAGTAAATCTTCCTCGTCGTGGGAGACATATTTTTTTTATCCTTAACCAATCCTCTCCACAGAATTTTTGCCCAGCAGTCATCAACTTGGAGAGATAAGCATGTGGCCCCAATCCAAAAAACTTTGATGTGTGAAAGAAATTTTCTGTAGCCAAAAATTTCCCCTGAAGCTTAGGTATAAAAGTCGATCCTATGTCACAATGCTGATAAGAACACGACTCTTTCCCCTTTTGTAATAGGGTCAATGCTGCAGACCTGCACGCAGAAAAGTTTCCCCTTGACTGAACAGTGGGCTGATATTCGCTTTCTTCACTATGTGAGCTAGGCGAGGACTTCCACGAATCCACATTATAAGAGTACCCTGCCGGAGTACAAGGATCTATGTGCAACCCATCCTTAATATATTGAGAAGCCAGGTTTGAATCTCCTGATATAAGTGCTTCTCTCCATGAGTCATGAGCAACATTCAGGCCAAAATGAAGCAAGCTGTGGCTGTAAAGGCTGTAAGTGATATTCCCAAATTTAACAGTGCGTGAGAACAAAGGCAGCATTGCTTCTCTTGATACGAAGGTTACCTGAGCAGAAGCACCACCAAGTTCGATAATCCCAGTTGTCTCCAACGGATCACCTCCAAGAGTTCCCAGTGCATAATTAGCAACAACCCAGGCATACACTCCTTCATCAGAACCTGTTATAACAGAAGCCCAATCATCACTAAACTTGAATCCAGAAGAACGCAAAACACTCCTACAAGACTCTAGAATAGTCCGCTGCACTTCAACATCCAGCATCCTCATCCCAGCAGTAGCCATAAGCCGAATCTCCGTCTCCCTCCACCTCTCCTTGGGGATCCGATGTTTCGCAAACTCCACCAGCTCCGACAGCGAACTACCCGCTCCGTCTGGATCCTCCGCAAACGACGATAAACCTGGATTCACTCTCATCGAAACCACACCCTTTTTCCCAAAATCCAACGCGTTTGTAACCCTATACTTAAACACATGAACCCTAGTTCCCGTGCTTCCACCGTCGACGATTATAGTGTAACGGAAACTGGTTCTGGCTCCCGATGATGAGGAAGAGAAGAAGGTGTAGTATGAAACGATGAGGAGGAACGAGAAGAAGAGAGGACCGATGAACGTGGTCTTGAAACAGAGACACTTTGAACTGTAGTTGCTATTGCTGTTACGAGCGAAGAGATTGGTGGAGCGGACTTGGATCCGTTTCACGGTTGGATCCATTTGGTTTGGATTTGAGCCGACCCGATTGCGAGCATTCAATCGTCGCATCAAATGGAATTCGGAAGACGGAGGAGGAAGGAGGAATTGTTAACCATATCAAGGGGTGGAGGTGTGCCGGAGATGTTGGCGGCGGTGGTAGCTCTGACCTCTCTCCAGGCGCAGACGGAGAGAGAGAGAGGAGAGAAATTTGCGGAGTCCTCGCGTA
TARGET=2196,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2143
PRIMER_LEFT_PENALTY=0.054900
PRIMER_RIGHT_PENALTY=1.159363
PRIMER_LEFT_SEQUENCE=GTTAACCATATCAAGGGGTGGA
PRIMER_RIGHT_SEQUENCE=AGGACTCCGCAAATTTCTCTC
PRIMER_LEFT=2116,22
PRIMER_RIGHT=2228,21
PRIMER_LEFT_TM=59.945
PRIMER_RIGHT_TM=59.841
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=113
PRIMER_PAIR_PENALTY_1=1.2143
PRIMER_LEFT_1_PENALTY=0.054900
PRIMER_RIGHT_1_PENALTY=1.159363
PRIMER_LEFT_1_SEQUENCE=GTTAACCATATCAAGGGGTGGA
PRIMER_RIGHT_1_SEQUENCE=GAGGACTCCGCAAATTTCTCT
PRIMER_LEFT_1=2116,22
PRIMER_RIGHT_1=2229,21
PRIMER_LEFT_1_TM=59.945
PRIMER_RIGHT_1_TM=59.841
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=1.2277
PRIMER_LEFT_2_PENALTY=0.068302
PRIMER_RIGHT_2_PENALTY=1.159363
PRIMER_LEFT_2_SEQUENCE=TTAACCATATCAAGGGGTGGAG
PRIMER_RIGHT_2_SEQUENCE=AGGACTCCGCAAATTTCTCTC
PRIMER_LEFT_2=2117,22
PRIMER_RIGHT_2=2228,21
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=59.841
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=112
PRIMER_PAIR_PENALTY_3=1.2277
PRIMER_LEFT_3_PENALTY=0.068302
PRIMER_RIGHT_3_PENALTY=1.159363
PRIMER_LEFT_3_SEQUENCE=TTAACCATATCAAGGGGTGGAG
PRIMER_RIGHT_3_SEQUENCE=GAGGACTCCGCAAATTTCTCT
PRIMER_LEFT_3=2117,22
PRIMER_RIGHT_3=2229,21
PRIMER_LEFT_3_TM=60.068
PRIMER_RIGHT_3_TM=59.841
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=1.6293
PRIMER_LEFT_4_PENALTY=0.054900
PRIMER_RIGHT_4_PENALTY=1.574405
PRIMER_LEFT_4_SEQUENCE=GTTAACCATATCAAGGGGTGGA
PRIMER_RIGHT_4_SEQUENCE=GAGGACTCCGCAAATTTCTCTC
PRIMER_LEFT_4=2116,22
PRIMER_RIGHT_4=2229,22
PRIMER_LEFT_4_TM=59.945
PRIMER_RIGHT_4_TM=61.574
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=114
=
PRIMER_SEQUENCE_ID=SSR_03922 Ps_contig_mira-and-tgicl-ass_franssen_31503
SEQUENCE=TTACTTACACAAAACACAAACATTTCTTATTTAAAACAAATCATATTAAACCAACAGCAGTCTNAAACCCTAAACTACACTCTAATTTCCAATAAACAAAAGACAAAACAAAAGACTCACTCACTCAACTTCAAAACAAAGAAAACATTGATGCACCCTTAAATCAACTTATACTTATGCTCCTCAATGGTTCTTCTGCTGTTAAATCTGAATTCAGCTTTTCTGTTGTTAATTCTGAAACCGGCGTTTCTGCTGATATTTCTGCCCTTGGTTTGTATACAGATCTAAGAAAGTTATACGCAAGTGCTCCAGCTACAGCTCCAACAATTGGACCAACTACATATATCCATAAACCATTGTAAATATGCACCACAATTGCAGGTCCAATACTTCTTGCTGGATTCATAGATGCACCTGAAACAGGTCCAGCTATAAAAAGATTCAACGTTATCGTCATTCCAACCGCAATACTCGCTGAATCATCCACCGCTCTATCGTCTGTTGTAACAGCTGAAATAACAAACATTAACAGAAAACTGATAATGAATTCCATAGCTAAAGATTGACCACTTGATCCAACCGGCACAGTTCCAAAATAAGATTTTGGTGTAATATCAAACATTAAAGATAATGTTAAACTCGCAAGTGTTGAACCGAGTAACTCAGCAGCAATGTAAAGTGGAGCTTCGATGATTTTGATTCTGCGAAAGATGGCCCAGGTTATCGTCACCGCGGGGTTGAAGAGTCCTCCGGAGATATGGCCAACAGAATAACACATAACGGTTACAATCAAACCCCAAGTGATACATATCCCTGGAAATGTTATGGAGCCATAGATTTTGTCCACTGCTACAACTCCACACCCTGCAAATACAAGAAAATATGTTCCAATAACTTCTGCTATAACCTTCTGTATTAAGGTTATAGCACGGTTGGATGAACAACAAAGTTTCAAGATGGAATGGTTTCCTTGATGTATGATTGCCATTGATATGGATGTTCCCGAA
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1469
PRIMER_LEFT_PENALTY=0.085505
PRIMER_RIGHT_PENALTY=0.061428
PRIMER_LEFT_SEQUENCE=CCAACAGCAGTCTNAAACCCTA
PRIMER_RIGHT_SEQUENCE=TAGCTGGAGCACTTGCGTATAA
PRIMER_LEFT=50,22
PRIMER_RIGHT=314,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=5.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.75
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.1878
PRIMER_LEFT_1_PENALTY=0.085505
PRIMER_RIGHT_1_PENALTY=0.102276
PRIMER_LEFT_1_SEQUENCE=CCAACAGCAGTCTNAAACCCTA
PRIMER_RIGHT_1_SEQUENCE=GGGCAGAAATATCAGCAGAAAC
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=266,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.3095
PRIMER_LEFT_2_PENALTY=0.085505
PRIMER_RIGHT_2_PENALTY=0.224014
PRIMER_LEFT_2_SEQUENCE=CCAACAGCAGTCTNAAACCCTA
PRIMER_RIGHT_2_SEQUENCE=AAGGGCAGAAATATCAGCAGAA
PRIMER_LEFT_2=50,22
PRIMER_RIGHT_2=268,22
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=60.224
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.5718
PRIMER_LEFT_3_PENALTY=0.085505
PRIMER_RIGHT_3_PENALTY=0.486257
PRIMER_LEFT_3_SEQUENCE=CCAACAGCAGTCTNAAACCCTA
PRIMER_RIGHT_3_SEQUENCE=TGTATACAAACCAAGGGCAGAA
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=280,22
PRIMER_LEFT_3_TM=59.914
PRIMER_RIGHT_3_TM=59.514
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=231
PRIMER_PAIR_PENALTY_4=0.7155
PRIMER_LEFT_4_PENALTY=0.085505
PRIMER_RIGHT_4_PENALTY=0.629991
PRIMER_LEFT_4_SEQUENCE=CCAACAGCAGTCTNAAACCCTA
PRIMER_RIGHT_4_SEQUENCE=CAGAAGAACCATTGAGGAGCAT
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=197,22
PRIMER_LEFT_4_TM=59.914
PRIMER_RIGHT_4_TM=60.630
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.75
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_03923 Ps_contig_mira-and-tgicl-ass_franssen_31507
SEQUENCE=GTTCGGGGAAAAATATCCATATGGAATTTGCAATGNAAGGTGATCTTTGTTTCTGGTTCGCCACGTATTCAACTTCATCTTGGCGCCAGCCACAAACAAGTCACCTAACAGAAAAACAGAACTCTCTCTCTCTCCAATGGCTCTCACTCTCCGAGTTGTAAATGCAAATATAGGGTTTGACTCATCTACTCGTTCACCTTTTGTTTCACCTCGTAAACGCTTCGTGATTCGGTGCTCCCAAGTTCAGCAATTATCCATTACACAAGAATCTGAGAACTCACTCATCGAAGCACTTGTTGGCATCCAAGGACGAGGACGTTCTTCTTCCCCCAATCAACTCAACGCTATTGAACGTGCTATTCAAGTTCTGGAGACTTTAGGGGGTGTGTCTGATCCTACAAATTCAAGTTTGATTGAGGGTCGCTGGCAGCTCATTTTTACTACAAGACCTGGAACTGCATCCCCCATTCAGGCAGCAGCATCCATCAAAGATGGAAAAAGAATCCTTTTTAGGTTTGACAGAGCTGCTATTTTTCTTTTTAAATTTTCTTACCAATTTTAAAAGTTCCATATCCAGTTCCATTTAAGCTACTTGGAGATGAAG
TARGET=122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1081
PRIMER_LEFT_PENALTY=0.073989
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=GCCACAAACAAGTCACCTAACA
PRIMER_RIGHT_SEQUENCE=GGATAATTGCTGAACTTGGGAG
PRIMER_LEFT=88,22
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.1763
PRIMER_LEFT_1_PENALTY=0.073989
PRIMER_RIGHT_1_PENALTY=0.102276
PRIMER_LEFT_1_SEQUENCE=GCCACAAACAAGTCACCTAACA
PRIMER_RIGHT_1_SEQUENCE=GATAATTGCTGAACTTGGGAGC
PRIMER_LEFT_1=88,22
PRIMER_RIGHT_1=254,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.2374
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=CAGCCACAAACAAGTCACCTAA
PRIMER_RIGHT_2_SEQUENCE=GGATAATTGCTGAACTTGGGAG
PRIMER_LEFT_2=86,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.2675
PRIMER_LEFT_3_PENALTY=0.073989
PRIMER_RIGHT_3_PENALTY=0.193532
PRIMER_LEFT_3_SEQUENCE=GCCACAAACAAGTCACCTAACA
PRIMER_RIGHT_3_SEQUENCE=TGAATAGCACGTTCAATAGCGT
PRIMER_LEFT_3=88,22
PRIMER_RIGHT_3=362,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.806
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=0.2780
PRIMER_LEFT_4_PENALTY=0.073989
PRIMER_RIGHT_4_PENALTY=0.203971
PRIMER_LEFT_4_SEQUENCE=GCCACAAACAAGTCACCTAACA
PRIMER_RIGHT_4_SEQUENCE=ATGGATAATTGCTGAACTTGGG
PRIMER_LEFT_4=88,22
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=60.204
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_03924 Ps_contig_mira-and-tgicl-ass_franssen_31515
SEQUENCE=CGACGATAAATCCAAACATTTTCGCACCAGAAAAATAGAAAACCCGCACACACCCTCATCCACTATCCATATATCCGAATCATCATCACAGAATCACACACACATAGATGGGAACTGGTTCATCCAAGGAAACCGACGGCGGTTCTGAAGGAAGCAAAGACAGACTCCACGACGATGATCAATCAGGTCGACAACTCTACGTTTCTCTCAAGATGGAGAATCACAAACTCACTGCTCACCTTGTTCCTCACGTCGTTGGCTCTTGGGAGTCTTCCAAAGCTCTTCCTTTGGAGCGAGAATCGGTTTCAATGTGGGAACTTAGCTTTGTTGTTCCACCTAATCACGAAACTTTGGATTTCAAATTCTTATTGAAGCCTAAAGATAGCGATGTGCCTTGTTTTGTTGAGGAGGGTCCTAGCCGCCAACTTATTGGGGGAGCGCTGGGGGAGGATGCCAGACTTGCACTGTTTAAGGTTGATAGTGGTGAGGTTCTTGAGTATCAAGTATTTGTTAAAGCAGACAGGGTTTCTCCCTTTGACCTTGCGGCTAGTTGGAGGGCTTACCAGGAGAATTTCCGTCCTTCGGCCGTACGTGGGATTCCCGATGTCAGCATAAGCTCTACACCACAGACAGGTGGCGAGAATGGTTCTTCTGCAGGTTTGGAACTTGATCTTGAGCATTATGTTGTCCCAGCTCCCTCAACCTCTGCAAATTCAGCCCATGTATATGCAGCCAACAATACAGAGAATCCACGGTCACTAATTAGTGGGTCTGATGGAAGTTCATCTTCCACTAAGGAAATGGAGGTTGTTGTGCCAGATCCATCTAAGGTGTTTCAGAGTTCGGGAATGGTTGAATCCAAGTCAGTGGGAACATTTTCACCTCTGCAGAAGCACGATAGTCAGAAAGGACATTTTGTTGATAGAGGTGTTGGATCTCCTAGACTTGTAAGATCTTCTAGTTCAAATATTTTCTCGGGTGATAATTTGGGCACTGGTACCAAGAATTCAATGCCAGCTGCTGCTGGAGCTGTTGCAGCTGCAGCTGTTGCTGATCAGATGCTGGGGCTAAAGGAGGATAGACATTTGGCGATTGTCCTGGTGGGTCTGCCAGCTCGAGGTAAAACTTTTACTGCGGCTAAACTTACAAGATATCTTCGTTGGTTAGGTCATGATACCAAACACTTCAATGTCGGGAAGTATCGCCGCCTTAAGCATGGAGCTAATCAGTCTGCTGATTTCTTTCGAGCTGACAATCCAGAAGGCATGGAGGCACGTGATGAGGTAGCAGCGCTGGCATTTGAAGATATGTTAT
TARGET=95,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0504
PRIMER_LEFT_PENALTY=0.003602
PRIMER_RIGHT_PENALTY=0.046801
PRIMER_LEFT_SEQUENCE=CGCACCAGAAAAATAGAAAACC
PRIMER_RIGHT_SEQUENCE=AAGGTGAGCAGTGAGTTTGTGA
PRIMER_LEFT=22,22
PRIMER_RIGHT=241,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.953
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.0504
PRIMER_LEFT_1_PENALTY=0.003602
PRIMER_RIGHT_1_PENALTY=0.046801
PRIMER_LEFT_1_SEQUENCE=CAGAAAAATAGAAAACCCGCAC
PRIMER_RIGHT_1_SEQUENCE=AAGGTGAGCAGTGAGTTTGTGA
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=241,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.953
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=215
PRIMER_PAIR_PENALTY_2=0.0809
PRIMER_LEFT_2_PENALTY=0.003602
PRIMER_RIGHT_2_PENALTY=0.077340
PRIMER_LEFT_2_SEQUENCE=CGCACCAGAAAAATAGAAAACC
PRIMER_RIGHT_2_SEQUENCE=GTGGAGTCTGTCTTTGCTTCCT
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=169,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=59.923
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=0.1262
PRIMER_LEFT_3_PENALTY=0.003602
PRIMER_RIGHT_3_PENALTY=0.122569
PRIMER_LEFT_3_SEQUENCE=CAGAAAAATAGAAAACCCGCAC
PRIMER_RIGHT_3_SEQUENCE=GCTAAGTTCCCACATTGAAACC
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=322,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=59.877
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.1626
PRIMER_LEFT_4_PENALTY=0.115779
PRIMER_RIGHT_4_PENALTY=0.046801
PRIMER_LEFT_4_SEQUENCE=GAAAAATAGAAAACCCGCACAC
PRIMER_RIGHT_4_SEQUENCE=AAGGTGAGCAGTGAGTTTGTGA
PRIMER_LEFT_4=29,22
PRIMER_RIGHT_4=241,22
PRIMER_LEFT_4_TM=59.884
PRIMER_RIGHT_4_TM=59.953
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_03925 Ps_contig_mira-and-tgicl-ass_franssen_31517
SEQUENCE=ATAAATATTAATACGCTAATTTTTACTAGCATTTATAACAAGAAAATAAATTATTATTTTGATAAAACAATGAAGTAAAGTATTACTACAATGTAATTACATAAAAAACAACCACAGTAAAAAAACGTTTATACTGAACCAATCAATCAGCCTCTTGCAATCCACCTAGCACAACCGACCCAGCGAACAAACCAACAGCGCCGACACCAAGAACCGCCGCCACGACGGCCTGAACAACAACCAGAGCAGTAGAATCATCCGGAATCAGAACAACCGCGGCAACAGCAGCAACAAGCACAGGAAGCGAAAGAGAAGCCAAAACAGAAGGAGAAACAGTGGCGAATTTCTCCAATAAGCTGAGTAAACCAAGCTCTTCCGCCTTGGAAAACAAACCTAACTTCTCAATAGAAGATAGCGTTACGCCTAACTCCTCAGCTTTGGATAACAACCCTGCTTTCTCCACGTTGCTCAAAACTTTCCTCTTCTCGATTTTCTTGAATACGTCGACTTCAACCTCTTCGCTTCCCTCGTAGAATATCCCCGCTCCGAACCATTCTTTCTTCCAGTTGTCGTTGTACTTGTTCACCTTGTTTTTGGGAGCCATGGCGACGACGGTTAGAGGTAGTTTGTTGTTACGGGAGGGAGTGAGGAATGAGGTTTGGTGACGGAAGATGCGGTTGTTTGTGTGAAGATGACGAAGACAGAGAGGTGAGGTTGGAGCAGGCACCGCCATGGATAGATGATTGATTGATGATGCGCGCGCTTGAATCTCAATCTCAACTGCCACGTCGGATAATCAACAGAGTAAAGTAGTGAGATAGACATTTGTGTTTGTTACTAGTACGTGAGATCTTGCTGCTTCATTTTCCACCACTCGTATTGTTCCTAGTCCTACGTTCNTCYATCTTCTTTCTCTATCTTTTCCTTCCNCNRCTT
TARGET=741,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03926 Ps_contig_mira-and-tgicl-ass_franssen_31519
SEQUENCE=GAAGCAGTATGTGTCCAAATTAAGGAGAAATCCATCCCCCTTTTCGGAAACCTAACAAACCCAAACCAGTAACGTTTTCATCTCCGAATTCAGACAAACAGACACTAATTTTTACTACTATTTATTACTCTTTCTGTTCCTTTTCAATCTCAACTCAATAACCCTTTTCTTCCTATATATTCCTTGAGATTTAGTTGTATCCAGAACATGGATCATTTACAGAGGAAGCTACGCGACCACGAAGCTGCTATGTTTCAACAGGGTTTTCTGGATGATCAGTTCAGTCAGCTTCAGAAACTTCAAGATGACAGTAGTCCAGATTTTGTGTACGAGGTTATCACTTTGTTCTTTGCTGATTCTGATAAGCTTCTCAACAACATGTCACATGAACTAGCACAAGTACCTGTGAATTTCAAACAAATTGATGCTCATGCTCATCAACAGAAGGGAAGCAGTGCTAGTGTTGGTGCTGCTCGAGTTACAAGTATATGTGCCACCTTCAGAAGTTTCTGTGAGGCTCAGAACCTCGAAGGGTGTCGTAGATGTCTACAACAATTGCAACACGAATACAAACTACTGAAGACCCATCTTCATTACCTATTTAAGCTGCAACGAGATATCAAGGCTTTCGGCCGTTCTATTCCTACGAGGGAGTAAGAGATTACATGATTGCCGACGGTTGATATTGAAAAAGATAACGAAGCTTTCTATGTAGCTGCTGATTTTGTTTGTTTTGGTTTTTGTTGGATTGTTCCTTCAGAATTAGAGCTAGTTTGAATGTTGGGATAGTGTTGATGCGAATGCGCATTTCTTGCTTAGTTTGATAGATATATATATGATATTTTATATAATTTTTCCCARTAATCTGGAGATTTATTTGAAGTTTTATGTTTACTCAAAATAAGATTAAAGTTTAACAAAATT
TARGET=828,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03927 Ps_contig_mira-and-tgicl-ass_franssen_31520
SEQUENCE=ACATACATAATACACAAAATCTAACACACCCTTCATCACTTGCTATATATATACCTATCTCCACATTCTCATGCTCCCGAACTCTCTTCTCTTTCTTTCCTACCCTTCCTATCTCTCTGCATACTTCAGCTACATAAGAGTTAACCACAAAGAAAGTTTACATGGTTGATATTCAAATCCCAAGCACATTCGACCCGTTCGCGGAGGCCAAAGAATCAGATGCACCGGGTACAAAAGAGTATGTGCATATTCGCATACAGCAGCGGAATGGAAAGAAGAGCCTAACCACAGTTCAAGGGCTGAAGAAGGAGTTCAGCTATGAGAAGATCTTAAAGGATCTCAAGAAAGAGTTTTGTTGCAACGGAAACGTCGTCAACTGTAAGGATCTCGGCAAGATTATCCAACTCCAAGGCGATCAGCGCAAGAATGTTTCGCACTTTCTTGTTCATGCCGGTCTCGTCCGAAAGGATAACATCAAGATTCATGGTTTTTGATCAAGGTTATAGTTCATGTTTGTGTTTTCTACATGCATGCTTGTATTAAATAATTAAGCATAAAAAATCTTGTATAATTGATATAATCTTAATGAGAAAATCACCTTTCCTAGGTGAGTTCATACTTTATGAGTGTTTGTAACCAAAAAAAAAAAAAAAACAAAAAAGTACTCTGTCG
TARGET=44,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0909
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.053515
PRIMER_LEFT_SEQUENCE=TAACACACCCTTCATCACTTGC
PRIMER_RIGHT_SEQUENCE=TGCACATACTCTTTTGTACCCG
PRIMER_LEFT=21,22
PRIMER_RIGHT=246,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.054
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=11.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=226
PRIMER_PAIR_PENALTY_1=0.1884
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.151106
PRIMER_LEFT_1_SEQUENCE=TAACACACCCTTCATCACTTGC
PRIMER_RIGHT_1_SEQUENCE=TAGCTGAACTCCTTCTTCAGCC
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=318,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.2269
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.189529
PRIMER_LEFT_2_SEQUENCE=TAACACACCCTTCATCACTTGC
PRIMER_RIGHT_2_SEQUENCE=CCTTGAACTGTGGTTAGGCTCT
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=297,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=59.810
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.6264
PRIMER_LEFT_3_PENALTY=0.037339
PRIMER_RIGHT_3_PENALTY=0.589035
PRIMER_LEFT_3_SEQUENCE=TAACACACCCTTCATCACTTGC
PRIMER_RIGHT_3_SEQUENCE=CTGTGGTTAGGCTCTTCTTTCC
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=290,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=59.411
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.7734
PRIMER_LEFT_4_PENALTY=0.037339
PRIMER_RIGHT_4_PENALTY=0.736104
PRIMER_LEFT_4_SEQUENCE=TAACACACCCTTCATCACTTGC
PRIMER_RIGHT_4_SEQUENCE=TGGTTAGGCTCTTCTTTCCATT
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=287,22
PRIMER_LEFT_4_TM=60.037
PRIMER_RIGHT_4_TM=59.264
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_03928 Ps_contig_mira-and-tgicl-ass_franssen_31527
SEQUENCE=CTCGAAACCCTAAACTACTAACCCTAAACCAACTNGGCAGCAGATGCCTCCGAAGCAATCAAAAGCTGATGTAGCGAAGAAGCAGAAGGTTGTTGAAGACAAAACTTTTGGTCTCAAGAACAAAAACAAAAAGCAAAGCAGTTCAGAAATATGTTCAGAATCTTAAGTCTTCCGTGCAGCCCAAAACCGACCCCAAAGTCGATGCAAAGAAAAAGAGGGAGGAAGAGAAGGCAAAAGAGAAGGAGCTGAATGATTTGTTCAAAATTGCTGTCAGTCAGCCTAAAGTCCCAGCTGGTGTTGATCCCAAGTCTATATTATGTGAGTTTTTTAAAGTGGGTCAGTGTGCTAAGGGCTTCAAATGCAAATTTTCCCATGATTTGAATGTTCAGAGGAAAGGAGAAAAGATTGACATATATAGTGATAAGCGCGATGATGATACAATGGAGGAATGGGATCAAGCGACATTGGAGAAAGTGGTAGAGTCGAAGCAAAATGAGTATAATCAGAACAAACCAACTGATATTGTATGCAAACACTTTTTAGATGCGGTTGAGAGGAAGCAATATGGTTGGTTTTGGGCATGTCCCAATGGCGGTAAGAATTGTATCTATAGACATGCACTTCCACCTGGATATATTTTAAAATCCCAAATGAAGGCTTTGTTGGAGGAGGAGTCTGATAAAATGCCAATTGAGGAGGAGATTGAAAAGCAGCGTGCCCAAGTGAAAACTACAACCCCTATGTCTACTGAGTTATTCCTCCAATGGAAGAAGAAAAAGATTGATGAGAGAGAGGCTAGTTTGGCTGCCCAACAGGCAGAGAGGGCTAAGAATGACCGCATGAGTGGCCGTGAGCTGTTTCTGTCAGATGCTAGCTTGTTTGTGGATGATGCTGAAGCATATGATAAGTACCATAGACAACCTGAATCTGATGAAACTGAACAGAATGGGACTGGGAGTGCCGCTAATAATGGTCCCAGCACCTCAGCAGCAGGTGGCGCTGATGATGAACTTCCTGATGATATTGACGACGACGATGATGAATTGGACTTGGATGAGTTGGATGAACTGGAAGCTAGTTTAGCCAAGACATCAATCCACATTAAGGAAGCTGAAGCTTAATCCTGAGAAATAATCTTGACCTTGATGTGATATTTTTATGCATCTGCTGAACAATATATCATTTGCTTCTAGCCACTGGCATTCCTTCCATTTGTGGAAGTTTTTCATATGAAGCCTTTGATGATGAAATCACTATTTGCAGCATTGCATTTGGTATGAATGATATGACAGTTTGGTACGATTCGATTTTCTGTAGTTAGGTCACTCGTTGATCCTGCGACTATGACACTTTTTTGTTGTTTGAATATGGGTAGTCTTTGTATAGACTGTACCTAATTTTAGTTATTTGAGTTTCCGCCGCATAGAGTGATTGCAGTTGTGCCGTGTATGTTGCAGCTAGATATGTCCCTAGGGAGTTAGGGTGTGTGTGTTTGCGGTTGAAAGAAAATGAAGAGGAAAGCAATAAGAGGGATTAAGATCATCTAAGCTTCCTCTCATTCAAAAGTAAAGGGAAGAAGAGGAGATGGAGAAAGGGAAGGGAGTTTCATCCCTGAAAATTTCTGCTGATTTATGAAAAACAAGATGAGATAATAGAAAATGTTTGGTTTGCAAAATAT
TARGET=1483,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2897
PRIMER_LEFT_PENALTY=0.236656
PRIMER_RIGHT_PENALTY=0.053000
PRIMER_LEFT_SEQUENCE=TGATCCTGCGACTATGACACTT
PRIMER_RIGHT_SEQUENCE=ATGAAACTCCCTTCCCTTTCTC
PRIMER_LEFT=1330,22
PRIMER_RIGHT=1608,22
PRIMER_LEFT_TM=59.763
PRIMER_RIGHT_TM=59.947
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.2897
PRIMER_LEFT_1_PENALTY=0.236656
PRIMER_RIGHT_1_PENALTY=0.053000
PRIMER_LEFT_1_SEQUENCE=TGATCCTGCGACTATGACACTT
PRIMER_RIGHT_1_SEQUENCE=GATGAAACTCCCTTCCCTTTCT
PRIMER_LEFT_1=1330,22
PRIMER_RIGHT_1=1609,22
PRIMER_LEFT_1_TM=59.763
PRIMER_RIGHT_1_TM=59.947
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.2897
PRIMER_LEFT_2_PENALTY=0.236656
PRIMER_RIGHT_2_PENALTY=0.053000
PRIMER_LEFT_2_SEQUENCE=TTGATCCTGCGACTATGACACT
PRIMER_RIGHT_2_SEQUENCE=ATGAAACTCCCTTCCCTTTCTC
PRIMER_LEFT_2=1329,22
PRIMER_RIGHT_2=1608,22
PRIMER_LEFT_2_TM=59.763
PRIMER_RIGHT_2_TM=59.947
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.2897
PRIMER_LEFT_3_PENALTY=0.236656
PRIMER_RIGHT_3_PENALTY=0.053000
PRIMER_LEFT_3_SEQUENCE=TTGATCCTGCGACTATGACACT
PRIMER_RIGHT_3_SEQUENCE=GATGAAACTCCCTTCCCTTTCT
PRIMER_LEFT_3=1329,22
PRIMER_RIGHT_3=1609,22
PRIMER_LEFT_3_TM=59.763
PRIMER_RIGHT_3_TM=59.947
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.3388
PRIMER_LEFT_4_PENALTY=0.236656
PRIMER_RIGHT_4_PENALTY=0.102139
PRIMER_LEFT_4_SEQUENCE=TGATCCTGCGACTATGACACTT
PRIMER_RIGHT_4_SEQUENCE=CCTCTTCATTTTCTTTCAACCG
PRIMER_LEFT_4=1330,22
PRIMER_RIGHT_4=1516,22
PRIMER_LEFT_4_TM=59.763
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_03929 Ps_contig_mira-and-tgicl-ass_franssen_31528
SEQUENCE=AGACCAAAATAACCCCGACACTTCGTGGCTTTGTTTGTTACTGTCAGTACACAGAGAGAGAAAATAATCATATGCGAAAGAGTGAACAAGCTCTGATCTTTCTCCTCCGAATTCTAAATCTGTTGGCTTCAGTTGTTCAATTGAACAGACTGTTGCGGAGAGTGTGCTTTTGTAAAGTAATTTTATAGGTATATTAAAATAAGATTGGTATGGGAGAGGAAGAACCAGTGACTGAAGTTGCTAAGAGTGACTCAAATGGCGGCAGCATTGCTGCAAAGACATTAGATGAAGTTGTTGAAAAGAAAGACTTGGAAACTGATGGAGGGAAAGTCTTGGAGAGCAATGGAGTCAAAGAAATTGAAGATAAGAAAGCTGATGATGTGGAAGAGGTAAAAGAGGATGAGAATGCTGATATAGTAGAGGAAATAAAAGAGGATAAGAAAGATGATGTGGTGGGGGAAGTAAAAGAGGATAAGAAAGATAATGGGGCTGATGAAGTAAAAGAGGAGACGAAAGTTGATGGGGTGGGGGAAGTAAAAGAGGATAAGAAAGATGATGAGGCTGATGAAGTAAAAGAGGAGACGAAAGATGATGGTGTGGAAGAAGTAAAAGATGATAAGAAAGAGGATGTGGCTGAAGAAGTAAAAGAGGATAAGAAAGATGATGGGGTGGAAGAAGTAAAAGATGAGAAGAAAGATGATACGGTGGAAGAAGAAAAAGAAGATAGGAAAGTTGATGGGGTGGAAGAAGTAAAAGATGATGTGGTGGAAGAAGAAAAAGAGGATGAGAAAGTTGATGAGGTGGAAGAAGTAAAAGATGATGTGGTGGAAGAAGAAAAAGAGGATGAGAAAGTTGATGAGGTGGAAGAAGTAGAAGATGATGCGGCGGAAGAAGAAAAAGAGGATGAAAAAGTTGATGAGGTGGAAGAAGTAAAAGATGAAAGGGTGGAAGAAGTAAAGGAGGTTACGAAAGATGGTGAGGTGGGAGAAGGAAAGGAGGCTACAAACGATGGTGTGGTGGAAGAAATAAAAGAGGATAAAGAAATTGATGGTACTGAGGAAGTAAAGGTGGAAAAGAAAGATAATGGAGGCGAAGATGTTAAGGAGGATATGGAAGTTGATAGTGTAAAAGAAGTAGTGGAGGCTAAGAAAGATGATGATGACATTCAAGAAATAGAAGAGGATGACAAAAATGTTACCGAATCTGAGAATGAGAAAATGGATGTCGATACCGAGGTTAAGGAAACTACAGAAGGTAAAGAAGAAATGGAAAAAATTGAATCTGTAAAAGAAGAAGCAGATTTGATGGAAACAGAGGGTAGCGTTGAGGAAAAGGAAGAGGGCGATGAAGAGGAGAAAAATGAAGCAGAGATAGAGGAGAAGGAAGAGGATGATGAAAAGGAGGAATCTGAAGCAGAGACAGTGGAAAAGGAAGAGGGTGATGTAAAGGAGAAAACTGAAGCAGAGACAGAAGAGGAGGGGGAGGAGGAGGATGACAAGGATAGTATTGATAAATCAAAAGAAGAAGATAAGGCAGAGGGTAGTAAAGGTGAGAAAGGATCAAGAAAGCGTGCGAGAGGGAAGATTAACGAGGAGAAAGTGAAGGAGAAAATAAAGGAACTGAAACAGCTAGAGCCTAAGACTCCTACTAGTGATCGTCCAGTTCGAGAAAGAAAATCAGTTGAGAGGTTGGTAGCATTAATCGATAAAGACACGAGCAAAGTATTTCACATCGAGAAGGGTCGTGGCACACCTTTAAAAGATATACCCAATGTGGCATCTAAGTTATCTAGAAGGAAAAATGATGATAGTCTCAAGTTGCTCCACTCAATCCTCTTCGGGAGGAGAGGGAAGGCAGTTCAGGTCAAGAGTAATATATCGAGGTTTTCTGGTTTTGTATGGCATGGCAATGAGGAGAAGCAAATGCTTAAAGTAAAAGAAAAGCTAGACAAATGTACTAAAGAGAAGTTACTGGAATTCTGTGATGTGTTTGACATAACACTTAACAAGTCAATGAAGAAAAAAAAGGAAGATGTTATTACTAAGCTGATAGACTTTTTGCTTGCCCCTCATGCAACCACAACTGTGCTACTTGCAGAAAAAGAGTCCAGTAAGAAGCGCAAGCGTGTTGTGAAACGAGGTACATCAAGGCCTAGGACTAGTACTTCAAGAGGTTCTTCTGCAAAGAGGCAGAAGAATAATGAAAATTCTTCAGGAGTGGAGAAAAAGAGCACAACTGACACAGAAGATGAATCAGAGGGAGAGGAGAAAAATGAAGAAAATGAGGATGAAACCGAGAATGACATTCCTGAGAAATCTGAAGATGATATATCAGAGAAATCTGAAAGGGGAGATAARAGTGATTCGTGGGGAGTGAATCTGATGATGTAGAAATAAAAAGAGAAAACTATTACTAAATCATCATCTTACAAAAGAAAGAGTCCTCTGGAAGAAGCAAAATCGAGAAAACTACGGTCACAAATAAATCTCGCTCACCTCCTAAGAGAGCACCTAAGAAATCATCAAATACCCAACCGAAGTATGACGATGATATTGATGAAAGTCCAAAGGTATTTTCTAGGAAGAAGAAAAATGAGCAAGGGGGAAAGCAGAAGAATATCAACTCCAACTCCAACTAAGTCTTCATCAAAGTCCAAAGAAGAAAACTAGAAAAAGGTTGACTAAAGGGGAAAAAGGACAAGAAAGAAAGAGAAGTCCTC
TARGET=1481,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03930 Ps_contig_mira-and-tgicl-ass_franssen_31528
SEQUENCE=AGACCAAAATAACCCCGACACTTCGTGGCTTTGTTTGTTACTGTCAGTACACAGAGAGAGAAAATAATCATATGCGAAAGAGTGAACAAGCTCTGATCTTTCTCCTCCGAATTCTAAATCTGTTGGCTTCAGTTGTTCAATTGAACAGACTGTTGCGGAGAGTGTGCTTTTGTAAAGTAATTTTATAGGTATATTAAAATAAGATTGGTATGGGAGAGGAAGAACCAGTGACTGAAGTTGCTAAGAGTGACTCAAATGGCGGCAGCATTGCTGCAAAGACATTAGATGAAGTTGTTGAAAAGAAAGACTTGGAAACTGATGGAGGGAAAGTCTTGGAGAGCAATGGAGTCAAAGAAATTGAAGATAAGAAAGCTGATGATGTGGAAGAGGTAAAAGAGGATGAGAATGCTGATATAGTAGAGGAAATAAAAGAGGATAAGAAAGATGATGTGGTGGGGGAAGTAAAAGAGGATAAGAAAGATAATGGGGCTGATGAAGTAAAAGAGGAGACGAAAGTTGATGGGGTGGGGGAAGTAAAAGAGGATAAGAAAGATGATGAGGCTGATGAAGTAAAAGAGGAGACGAAAGATGATGGTGTGGAAGAAGTAAAAGATGATAAGAAAGAGGATGTGGCTGAAGAAGTAAAAGAGGATAAGAAAGATGATGGGGTGGAAGAAGTAAAAGATGAGAAGAAAGATGATACGGTGGAAGAAGAAAAAGAAGATAGGAAAGTTGATGGGGTGGAAGAAGTAAAAGATGATGTGGTGGAAGAAGAAAAAGAGGATGAGAAAGTTGATGAGGTGGAAGAAGTAAAAGATGATGTGGTGGAAGAAGAAAAAGAGGATGAGAAAGTTGATGAGGTGGAAGAAGTAGAAGATGATGCGGCGGAAGAAGAAAAAGAGGATGAAAAAGTTGATGAGGTGGAAGAAGTAAAAGATGAAAGGGTGGAAGAAGTAAAGGAGGTTACGAAAGATGGTGAGGTGGGAGAAGGAAAGGAGGCTACAAACGATGGTGTGGTGGAAGAAATAAAAGAGGATAAAGAAATTGATGGTACTGAGGAAGTAAAGGTGGAAAAGAAAGATAATGGAGGCGAAGATGTTAAGGAGGATATGGAAGTTGATAGTGTAAAAGAAGTAGTGGAGGCTAAGAAAGATGATGATGACATTCAAGAAATAGAAGAGGATGACAAAAATGTTACCGAATCTGAGAATGAGAAAATGGATGTCGATACCGAGGTTAAGGAAACTACAGAAGGTAAAGAAGAAATGGAAAAAATTGAATCTGTAAAAGAAGAAGCAGATTTGATGGAAACAGAGGGTAGCGTTGAGGAAAAGGAAGAGGGCGATGAAGAGGAGAAAAATGAAGCAGAGATAGAGGAGAAGGAAGAGGATGATGAAAAGGAGGAATCTGAAGCAGAGACAGTGGAAAAGGAAGAGGGTGATGTAAAGGAGAAAACTGAAGCAGAGACAGAAGAGGAGGGGGAGGAGGAGGATGACAAGGATAGTATTGATAAATCAAAAGAAGAAGATAAGGCAGAGGGTAGTAAAGGTGAGAAAGGATCAAGAAAGCGTGCGAGAGGGAAGATTAACGAGGAGAAAGTGAAGGAGAAAATAAAGGAACTGAAACAGCTAGAGCCTAAGACTCCTACTAGTGATCGTCCAGTTCGAGAAAGAAAATCAGTTGAGAGGTTGGTAGCATTAATCGATAAAGACACGAGCAAAGTATTTCACATCGAGAAGGGTCGTGGCACACCTTTAAAAGATATACCCAATGTGGCATCTAAGTTATCTAGAAGGAAAAATGATGATAGTCTCAAGTTGCTCCACTCAATCCTCTTCGGGAGGAGAGGGAAGGCAGTTCAGGTCAAGAGTAATATATCGAGGTTTTCTGGTTTTGTATGGCATGGCAATGAGGAGAAGCAAATGCTTAAAGTAAAAGAAAAGCTAGACAAATGTACTAAAGAGAAGTTACTGGAATTCTGTGATGTGTTTGACATAACACTTAACAAGTCAATGAAGAAAAAAAAGGAAGATGTTATTACTAAGCTGATAGACTTTTTGCTTGCCCCTCATGCAACCACAACTGTGCTACTTGCAGAAAAAGAGTCCAGTAAGAAGCGCAAGCGTGTTGTGAAACGAGGTACATCAAGGCCTAGGACTAGTACTTCAAGAGGTTCTTCTGCAAAGAGGCAGAAGAATAATGAAAATTCTTCAGGAGTGGAGAAAAAGAGCACAACTGACACAGAAGATGAATCAGAGGGAGAGGAGAAAAATGAAGAAAATGAGGATGAAACCGAGAATGACATTCCTGAGAAATCTGAAGATGATATATCAGAGAAATCTGAAAGGGGAGATAARAGTGATTCGTGGGGAGTGAATCTGATGATGTAGAAATAAAAAGAGAAAACTATTACTAAATCATCATCTTACAAAAGAAAGAGTCCTCTGGAAGAAGCAAAATCGAGAAAACTACGGTCACAAATAAATCTCGCTCACCTCCTAAGAGAGCACCTAAGAAATCATCAAATACCCAACCGAAGTATGACGATGATATTGATGAAAGTCCAAAGGTATTTTCTAGGAAGAAGAAAAATGAGCAAGGGGGAAAGCAGAAGAATATCAACTCCAACTCCAACTAAGTCTTCATCAAAGTCCAAAGAAGAAAACTAGAAAAAGGTTGACTAAAGGGGAAAAAGGACAAGAAAGAAAGAGAAGTCCTC
TARGET=2699,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03931 Ps_contig_mira-and-tgicl-ass_franssen_31529
SEQUENCE=GTCGGGGACTCATCACTCTAAAATCTAAATCCCATTCCTTCCCCTCTTTCACTATCATCCATGGCTCCCATCTCAAAACTCATCTCTTTCCTTTTCCTAACAACCCTTTCATTCTCTCTCCATATCCATGCCAGAGACAGTCAATTCTTCAGCAAAGTCACTCATGTCAACAACAATCTCAATGACAGACAAGTTCTCAATCCCAACAACAATGAACAACCATTAAACAAACCACAACAACAACAACAACAACAACCGGTTTTCATCCCAGAAACTGATAACAGTTATGGCTTATACGGCCATGAATCTGGTCTACACCCTCCCACAACAACCACCTCAAACAATGTCGTTCCCAACACTTACCAATCACAAGATGAAAACAACAATTACTTCACCACCTTCCAGAAGGATTCTTACACCAACAACAACAAGTATCCAAATGAAATCAGTGACACAAAACTCACACAAACTTCTTACAACAACAATAATAACAACAATTACGACAAGTATTACTACAACAACAACAAGTATGCTTCTAATGAACTTACCAACACAAAGTTCCCAGAAGAAGGATACAACTCCATGGAAAACCAGAACAACAAATATTACTACAACAACAATGTTGCTGCTAATGACAAATACTACTTCAAAACCAATGGCGCTAGTAACAACAATTACAACAACGTTGAAAGACAAGGAATGAGTGACACAAGAGCTATGGAAGGTGGGAAATACTTTTACGATGTTAACTCTGAGAAATACAACCCAGCATTATTCAATGGAGACTCGTCTAAAGGAGTTGTCAACTCTGAGAATTGGTACAACAAAAACGGTTACTTTGGTAACAACAACAATGTGAACAGTTATGAGAATAACAAGAATTCTAGGAATGGTTATCAGAATCAAGAGGAGTTTGAGAGTGACCAAGAAGAATTTGAGCCTTGAGATTGAAACAAATCCTTTTGAATTTTGGATGTGGTTGTTCAATGTTTTAATGTATTTTAGATTCAAAATCACAAAAAAAAAAGTTTAAAGATAGGGTAATTATATTATGAATGGGTTAATTTAGTGTTTTATTTACTTGGTTGGTGAAATTATATTAAGTCTTGTCTTGTTACTTATGAAATTTTCTGAAATTGCACAGTGTATGTATGTTATGATTTATGAAAGCATGTTTATTTTGTNGCTG
TARGET=234,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2474
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.239177
PRIMER_LEFT_SEQUENCE=CAAGTTCTCAATCCCAACAACA
PRIMER_RIGHT_SEQUENCE=CATGGCCGTATAAGCCATAACT
PRIMER_LEFT=189,22
PRIMER_RIGHT=303,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.239
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=0.2474
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.239177
PRIMER_LEFT_1_SEQUENCE=CAAGTTCTCAATCCCAACAACA
PRIMER_RIGHT_1_SEQUENCE=ATGGCCGTATAAGCCATAACTG
PRIMER_LEFT_1=189,22
PRIMER_RIGHT_1=302,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.239
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=0.3067
PRIMER_LEFT_2_PENALTY=0.067516
PRIMER_RIGHT_2_PENALTY=0.239177
PRIMER_LEFT_2_SEQUENCE=TCCTTCCCCTCTTTCACTATCA
PRIMER_RIGHT_2_SEQUENCE=CATGGCCGTATAAGCCATAACT
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=303,22
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=60.239
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=0.3067
PRIMER_LEFT_3_PENALTY=0.067516
PRIMER_RIGHT_3_PENALTY=0.239177
PRIMER_LEFT_3_SEQUENCE=TCCTTCCCCTCTTTCACTATCA
PRIMER_RIGHT_3_SEQUENCE=ATGGCCGTATAAGCCATAACTG
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=302,22
PRIMER_LEFT_3_TM=60.068
PRIMER_RIGHT_3_TM=60.239
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.4202
PRIMER_LEFT_4_PENALTY=0.008257
PRIMER_RIGHT_4_PENALTY=0.411917
PRIMER_LEFT_4_SEQUENCE=CAAGTTCTCAATCCCAACAACA
PRIMER_RIGHT_4_SEQUENCE=TAAGAATCCTTCTGGAAGGTGG
PRIMER_LEFT_4=189,22
PRIMER_RIGHT_4=415,22
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=59.588
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_03932 Ps_contig_mira-and-tgicl-ass_franssen_31529
SEQUENCE=GTCGGGGACTCATCACTCTAAAATCTAAATCCCATTCCTTCCCCTCTTTCACTATCATCCATGGCTCCCATCTCAAAACTCATCTCTTTCCTTTTCCTAACAACCCTTTCATTCTCTCTCCATATCCATGCCAGAGACAGTCAATTCTTCAGCAAAGTCACTCATGTCAACAACAATCTCAATGACAGACAAGTTCTCAATCCCAACAACAATGAACAACCATTAAACAAACCACAACAACAACAACAACAACAACCGGTTTTCATCCCAGAAACTGATAACAGTTATGGCTTATACGGCCATGAATCTGGTCTACACCCTCCCACAACAACCACCTCAAACAATGTCGTTCCCAACACTTACCAATCACAAGATGAAAACAACAATTACTTCACCACCTTCCAGAAGGATTCTTACACCAACAACAACAAGTATCCAAATGAAATCAGTGACACAAAACTCACACAAACTTCTTACAACAACAATAATAACAACAATTACGACAAGTATTACTACAACAACAACAAGTATGCTTCTAATGAACTTACCAACACAAAGTTCCCAGAAGAAGGATACAACTCCATGGAAAACCAGAACAACAAATATTACTACAACAACAATGTTGCTGCTAATGACAAATACTACTTCAAAACCAATGGCGCTAGTAACAACAATTACAACAACGTTGAAAGACAAGGAATGAGTGACACAAGAGCTATGGAAGGTGGGAAATACTTTTACGATGTTAACTCTGAGAAATACAACCCAGCATTATTCAATGGAGACTCGTCTAAAGGAGTTGTCAACTCTGAGAATTGGTACAACAAAAACGGTTACTTTGGTAACAACAACAATGTGAACAGTTATGAGAATAACAAGAATTCTAGGAATGGTTATCAGAATCAAGAGGAGTTTGAGAGTGACCAAGAAGAATTTGAGCCTTGAGATTGAAACAAATCCTTTTGAATTTTGGATGTGGTTGTTCAATGTTTTAATGTATTTTAGATTCAAAATCACAAAAAAAAAAGTTTAAAGATAGGGTAATTATATTATGAATGGGTTAATTTAGTGTTTTATTTACTTGGTTGGTGAAATTATATTAAGTCTTGTCTTGTTACTTATGAAATTTTCTGAAATTGCACAGTGTATGTATGTTATGATTTATGAAAGCATGTTTATTTTGTNGCTG
TARGET=420,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4963
PRIMER_LEFT_PENALTY=0.151106
PRIMER_RIGHT_PENALTY=0.345221
PRIMER_LEFT_SEQUENCE=AATGTCGTTCCCAACACTTACC
PRIMER_RIGHT_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_LEFT=342,22
PRIMER_RIGHT=571,22
PRIMER_LEFT_TM=60.151
PRIMER_RIGHT_TM=59.655
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.4963
PRIMER_LEFT_1_PENALTY=0.151106
PRIMER_RIGHT_1_PENALTY=0.345221
PRIMER_LEFT_1_SEQUENCE=GTCGTTCCCAACACTTACCAAT
PRIMER_RIGHT_1_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_LEFT_1=345,22
PRIMER_RIGHT_1=571,22
PRIMER_LEFT_1_TM=60.151
PRIMER_RIGHT_1_TM=59.655
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=227
PRIMER_PAIR_PENALTY_2=0.5485
PRIMER_LEFT_2_PENALTY=0.203324
PRIMER_RIGHT_2_PENALTY=0.345221
PRIMER_LEFT_2_SEQUENCE=CACAACAACCACCTCAAACAAT
PRIMER_RIGHT_2_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_LEFT_2=323,22
PRIMER_RIGHT_2=571,22
PRIMER_LEFT_2_TM=59.797
PRIMER_RIGHT_2_TM=59.655
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.5692
PRIMER_LEFT_3_PENALTY=0.224014
PRIMER_RIGHT_3_PENALTY=0.345221
PRIMER_LEFT_3_SEQUENCE=CTTATACGGCCATGAATCTGGT
PRIMER_RIGHT_3_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_LEFT_3=290,22
PRIMER_RIGHT_3=571,22
PRIMER_LEFT_3_TM=60.224
PRIMER_RIGHT_3_TM=59.655
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=0.5748
PRIMER_LEFT_4_PENALTY=0.151106
PRIMER_RIGHT_4_PENALTY=0.423703
PRIMER_LEFT_4_SEQUENCE=AATGTCGTTCCCAACACTTACC
PRIMER_RIGHT_4_SEQUENCE=GGTTTTCCATGGAGTTGTATCC
PRIMER_LEFT_4=342,22
PRIMER_RIGHT_4=591,22
PRIMER_LEFT_4_TM=60.151
PRIMER_RIGHT_4_TM=59.576
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=250
=
PRIMER_SEQUENCE_ID=SSR_03933 Ps_contig_mira-and-tgicl-ass_franssen_31529
SEQUENCE=GTCGGGGACTCATCACTCTAAAATCTAAATCCCATTCCTTCCCCTCTTTCACTATCATCCATGGCTCCCATCTCAAAACTCATCTCTTTCCTTTTCCTAACAACCCTTTCATTCTCTCTCCATATCCATGCCAGAGACAGTCAATTCTTCAGCAAAGTCACTCATGTCAACAACAATCTCAATGACAGACAAGTTCTCAATCCCAACAACAATGAACAACCATTAAACAAACCACAACAACAACAACAACAACAACCGGTTTTCATCCCAGAAACTGATAACAGTTATGGCTTATACGGCCATGAATCTGGTCTACACCCTCCCACAACAACCACCTCAAACAATGTCGTTCCCAACACTTACCAATCACAAGATGAAAACAACAATTACTTCACCACCTTCCAGAAGGATTCTTACACCAACAACAACAAGTATCCAAATGAAATCAGTGACACAAAACTCACACAAACTTCTTACAACAACAATAATAACAACAATTACGACAAGTATTACTACAACAACAACAAGTATGCTTCTAATGAACTTACCAACACAAAGTTCCCAGAAGAAGGATACAACTCCATGGAAAACCAGAACAACAAATATTACTACAACAACAATGTTGCTGCTAATGACAAATACTACTTCAAAACCAATGGCGCTAGTAACAACAATTACAACAACGTTGAAAGACAAGGAATGAGTGACACAAGAGCTATGGAAGGTGGGAAATACTTTTACGATGTTAACTCTGAGAAATACAACCCAGCATTATTCAATGGAGACTCGTCTAAAGGAGTTGTCAACTCTGAGAATTGGTACAACAAAAACGGTTACTTTGGTAACAACAACAATGTGAACAGTTATGAGAATAACAAGAATTCTAGGAATGGTTATCAGAATCAAGAGGAGTTTGAGAGTGACCAAGAAGAATTTGAGCCTTGAGATTGAAACAAATCCTTTTGAATTTTGGATGTGGTTGTTCAATGTTTTAATGTATTTTAGATTCAAAATCACAAAAAAAAAAGTTTAAAGATAGGGTAATTATATTATGAATGGGTTAATTTAGTGTTTTATTTACTTGGTTGGTGAAATTATATTAAGTCTTGTCTTGTTACTTATGAAATTTTCTGAAATTGCACAGTGTATGTATGTTATGATTTATGAAAGCATGTTTATTTTGTNGCTG
TARGET=515,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4963
PRIMER_LEFT_PENALTY=0.151106
PRIMER_RIGHT_PENALTY=0.345221
PRIMER_LEFT_SEQUENCE=AATGTCGTTCCCAACACTTACC
PRIMER_RIGHT_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_LEFT=342,22
PRIMER_RIGHT=571,22
PRIMER_LEFT_TM=60.151
PRIMER_RIGHT_TM=59.655
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.4963
PRIMER_LEFT_1_PENALTY=0.151106
PRIMER_RIGHT_1_PENALTY=0.345221
PRIMER_LEFT_1_SEQUENCE=GTCGTTCCCAACACTTACCAAT
PRIMER_RIGHT_1_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_LEFT_1=345,22
PRIMER_RIGHT_1=571,22
PRIMER_LEFT_1_TM=60.151
PRIMER_RIGHT_1_TM=59.655
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=227
PRIMER_PAIR_PENALTY_2=0.5485
PRIMER_LEFT_2_PENALTY=0.203324
PRIMER_RIGHT_2_PENALTY=0.345221
PRIMER_LEFT_2_SEQUENCE=CACAACAACCACCTCAAACAAT
PRIMER_RIGHT_2_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_LEFT_2=323,22
PRIMER_RIGHT_2=571,22
PRIMER_LEFT_2_TM=59.797
PRIMER_RIGHT_2_TM=59.655
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.5692
PRIMER_LEFT_3_PENALTY=0.224014
PRIMER_RIGHT_3_PENALTY=0.345221
PRIMER_LEFT_3_SEQUENCE=CTTATACGGCCATGAATCTGGT
PRIMER_RIGHT_3_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_LEFT_3=290,22
PRIMER_RIGHT_3=571,22
PRIMER_LEFT_3_TM=60.224
PRIMER_RIGHT_3_TM=59.655
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=0.5748
PRIMER_LEFT_4_PENALTY=0.151106
PRIMER_RIGHT_4_PENALTY=0.423703
PRIMER_LEFT_4_SEQUENCE=AATGTCGTTCCCAACACTTACC
PRIMER_RIGHT_4_SEQUENCE=GGTTTTCCATGGAGTTGTATCC
PRIMER_LEFT_4=342,22
PRIMER_RIGHT_4=591,22
PRIMER_LEFT_4_TM=60.151
PRIMER_RIGHT_4_TM=59.576
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=250
=
PRIMER_SEQUENCE_ID=SSR_03934 Ps_contig_mira-and-tgicl-ass_franssen_31530
SEQUENCE=TCACTCATTCCTTGTCTTTCAACGTTGTTGTAATTGTTGTTACTAGCGCCATTGGTTTTGAAGTAGTATTTGTCATTAGCAGCAACATTGTTGTTGTAGTAATATTTGTTGTTCTGGTTTTCCATGGAGTTGTATCCTTCTTCTGGGAACTTTGTGTTGGTAAGTTCATTAGAAGCATACTTGTTGTTGTTGTAGTAATACTTGTCGTAATTGTTGTTATTATTGTTGTTGTTGTAAGAAGTTTGAGTGAGTTTTGTGTCACTGATTTCATTTGGATACTTGTTGTTGTTGGTATAAGAATCCTTCTGAAAGGTGGTGAAGTAATTGTTGTTTTCATCTTGTGATTGGTAAGTGTTGGGAACGACATTGTTTGAGGTGGTTGTTGTGGGAGGGTGTAGACCAGATTCATGGCCGTATAAGCCATAACTGTTATCAGTTTCTGGGATGAAAACCGGTTGTTGTTGTTGTTGTGGTTTGTTTAATGGTTGTTCATTGTTGTTGGGATTGGGAACTTGTCTGTCATTGAGATTGTTGTTGACATGAGTGACTTTGCTGAAGAATTGACTGTCTCTGGCATGGATATGGAGAGAGAATGAAAGGGTTGTTAGGAAAAGGAAAGAGAGGAGTTTTGAGATGGGAGCCATAGATGATAGTGAAAGAGAAGAAGGAATGTGATTTAGATTTTAGAGTGATGAGTCCCCGAC
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3688
PRIMER_LEFT_PENALTY=0.345221
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_RIGHT_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT=135,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=59.655
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=0.4451
PRIMER_LEFT_1_PENALTY=0.421572
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=GTTGTTACTAGCGCCATTGGTT
PRIMER_RIGHT_1_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=60.422
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.4473
PRIMER_LEFT_2_PENALTY=0.423703
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=GGTTTTCCATGGAGTTGTATCC
PRIMER_RIGHT_2_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=319,22
PRIMER_LEFT_2_TM=59.576
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.4509
PRIMER_LEFT_3_PENALTY=0.427382
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=CTTCTGGGAACTTTGTGTTGGT
PRIMER_RIGHT_3_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT_3=139,22
PRIMER_RIGHT_3=319,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.4963
PRIMER_LEFT_4_PENALTY=0.345221
PRIMER_RIGHT_4_PENALTY=0.151106
PRIMER_LEFT_4_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_RIGHT_4_SEQUENCE=AATGTCGTTCCCAACACTTACC
PRIMER_LEFT_4=135,22
PRIMER_RIGHT_4=367,22
PRIMER_LEFT_4_TM=59.655
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_03935 Ps_contig_mira-and-tgicl-ass_franssen_31530
SEQUENCE=TCACTCATTCCTTGTCTTTCAACGTTGTTGTAATTGTTGTTACTAGCGCCATTGGTTTTGAAGTAGTATTTGTCATTAGCAGCAACATTGTTGTTGTAGTAATATTTGTTGTTCTGGTTTTCCATGGAGTTGTATCCTTCTTCTGGGAACTTTGTGTTGGTAAGTTCATTAGAAGCATACTTGTTGTTGTTGTAGTAATACTTGTCGTAATTGTTGTTATTATTGTTGTTGTTGTAAGAAGTTTGAGTGAGTTTTGTGTCACTGATTTCATTTGGATACTTGTTGTTGTTGGTATAAGAATCCTTCTGAAAGGTGGTGAAGTAATTGTTGTTTTCATCTTGTGATTGGTAAGTGTTGGGAACGACATTGTTTGAGGTGGTTGTTGTGGGAGGGTGTAGACCAGATTCATGGCCGTATAAGCCATAACTGTTATCAGTTTCTGGGATGAAAACCGGTTGTTGTTGTTGTTGTGGTTTGTTTAATGGTTGTTCATTGTTGTTGGGATTGGGAACTTGTCTGTCATTGAGATTGTTGTTGACATGAGTGACTTTGCTGAAGAATTGACTGTCTCTGGCATGGATATGGAGAGAGAATGAAAGGGTTGTTAGGAAAAGGAAAGAGAGGAGTTTTGAGATGGGAGCCATAGATGATAGTGAAAGAGAAGAAGGAATGTGATTTAGATTTTAGAGTGATGAGTCCCCGAC
TARGET=223,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3688
PRIMER_LEFT_PENALTY=0.345221
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_RIGHT_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT=135,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=59.655
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=0.4451
PRIMER_LEFT_1_PENALTY=0.421572
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=GTTGTTACTAGCGCCATTGGTT
PRIMER_RIGHT_1_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=60.422
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.4473
PRIMER_LEFT_2_PENALTY=0.423703
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=GGTTTTCCATGGAGTTGTATCC
PRIMER_RIGHT_2_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=319,22
PRIMER_LEFT_2_TM=59.576
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.4509
PRIMER_LEFT_3_PENALTY=0.427382
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=CTTCTGGGAACTTTGTGTTGGT
PRIMER_RIGHT_3_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT_3=139,22
PRIMER_RIGHT_3=319,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.4963
PRIMER_LEFT_4_PENALTY=0.345221
PRIMER_RIGHT_4_PENALTY=0.151106
PRIMER_LEFT_4_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_RIGHT_4_SEQUENCE=AATGTCGTTCCCAACACTTACC
PRIMER_LEFT_4=135,22
PRIMER_RIGHT_4=367,22
PRIMER_LEFT_4_TM=59.655
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_03936 Ps_contig_mira-and-tgicl-ass_franssen_31530
SEQUENCE=TCACTCATTCCTTGTCTTTCAACGTTGTTGTAATTGTTGTTACTAGCGCCATTGGTTTTGAAGTAGTATTTGTCATTAGCAGCAACATTGTTGTTGTAGTAATATTTGTTGTTCTGGTTTTCCATGGAGTTGTATCCTTCTTCTGGGAACTTTGTGTTGGTAAGTTCATTAGAAGCATACTTGTTGTTGTTGTAGTAATACTTGTCGTAATTGTTGTTATTATTGTTGTTGTTGTAAGAAGTTTGAGTGAGTTTTGTGTCACTGATTTCATTTGGATACTTGTTGTTGTTGGTATAAGAATCCTTCTGAAAGGTGGTGAAGTAATTGTTGTTTTCATCTTGTGATTGGTAAGTGTTGGGAACGACATTGTTTGAGGTGGTTGTTGTGGGAGGGTGTAGACCAGATTCATGGCCGTATAAGCCATAACTGTTATCAGTTTCTGGGATGAAAACCGGTTGTTGTTGTTGTTGTGGTTTGTTTAATGGTTGTTCATTGTTGTTGGGATTGGGAACTTGTCTGTCATTGAGATTGTTGTTGACATGAGTGACTTTGCTGAAGAATTGACTGTCTCTGGCATGGATATGGAGAGAGAATGAAAGGGTTGTTAGGAAAAGGAAAGAGAGGAGTTTTGAGATGGGAGCCATAGATGATAGTGAAAGAGAAGAAGGAATGTGATTTAGATTTTAGAGTGATGAGTCCCCGAC
TARGET=280,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3688
PRIMER_LEFT_PENALTY=0.345221
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_RIGHT_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT=135,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=59.655
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=0.4451
PRIMER_LEFT_1_PENALTY=0.421572
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=GTTGTTACTAGCGCCATTGGTT
PRIMER_RIGHT_1_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=60.422
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.4473
PRIMER_LEFT_2_PENALTY=0.423703
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=GGTTTTCCATGGAGTTGTATCC
PRIMER_RIGHT_2_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=319,22
PRIMER_LEFT_2_TM=59.576
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.4509
PRIMER_LEFT_3_PENALTY=0.427382
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=CTTCTGGGAACTTTGTGTTGGT
PRIMER_RIGHT_3_SEQUENCE=TTCACCACCTTTCAGAAGGATT
PRIMER_LEFT_3=139,22
PRIMER_RIGHT_3=319,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.4963
PRIMER_LEFT_4_PENALTY=0.345221
PRIMER_RIGHT_4_PENALTY=0.151106
PRIMER_LEFT_4_SEQUENCE=CCTTCTTCTGGGAACTTTGTGT
PRIMER_RIGHT_4_SEQUENCE=AATGTCGTTCCCAACACTTACC
PRIMER_LEFT_4=135,22
PRIMER_RIGHT_4=367,22
PRIMER_LEFT_4_TM=59.655
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_03937 Ps_contig_mira-and-tgicl-ass_franssen_31530
SEQUENCE=TCACTCATTCCTTGTCTTTCAACGTTGTTGTAATTGTTGTTACTAGCGCCATTGGTTTTGAAGTAGTATTTGTCATTAGCAGCAACATTGTTGTTGTAGTAATATTTGTTGTTCTGGTTTTCCATGGAGTTGTATCCTTCTTCTGGGAACTTTGTGTTGGTAAGTTCATTAGAAGCATACTTGTTGTTGTTGTAGTAATACTTGTCGTAATTGTTGTTATTATTGTTGTTGTTGTAAGAAGTTTGAGTGAGTTTTGTGTCACTGATTTCATTTGGATACTTGTTGTTGTTGGTATAAGAATCCTTCTGAAAGGTGGTGAAGTAATTGTTGTTTTCATCTTGTGATTGGTAAGTGTTGGGAACGACATTGTTTGAGGTGGTTGTTGTGGGAGGGTGTAGACCAGATTCATGGCCGTATAAGCCATAACTGTTATCAGTTTCTGGGATGAAAACCGGTTGTTGTTGTTGTTGTGGTTTGTTTAATGGTTGTTCATTGTTGTTGGGATTGGGAACTTGTCTGTCATTGAGATTGTTGTTGACATGAGTGACTTTGCTGAAGAATTGACTGTCTCTGGCATGGATATGGAGAGAGAATGAAAGGGTTGTTAGGAAAAGGAAAGAGAGGAGTTTTGAGATGGGAGCCATAGATGATAGTGAAAGAGAAGAAGGAATGTGATTTAGATTTTAGAGTGATGAGTCCCCGAC
TARGET=455,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2568
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.233224
PRIMER_LEFT_SEQUENCE=AATCCTTCTGAAAGGTGGTGAA
PRIMER_RIGHT_SEQUENCE=ATGACAGACAAGTTCCCAATCC
PRIMER_LEFT=298,22
PRIMER_RIGHT=522,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.233
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.2728
PRIMER_LEFT_1_PENALTY=0.203324
PRIMER_RIGHT_1_PENALTY=0.069526
PRIMER_LEFT_1_SEQUENCE=ATTGTTTGAGGTGGTTGTTGTG
PRIMER_RIGHT_1_SEQUENCE=ATCTATGGCTCCCATCTCAAAA
PRIMER_LEFT_1=365,22
PRIMER_RIGHT_1=647,22
PRIMER_LEFT_1_TM=59.797
PRIMER_RIGHT_1_TM=59.930
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.2728
PRIMER_LEFT_2_PENALTY=0.203324
PRIMER_RIGHT_2_PENALTY=0.069526
PRIMER_LEFT_2_SEQUENCE=ACATTGTTTGAGGTGGTTGTTG
PRIMER_RIGHT_2_SEQUENCE=ATCTATGGCTCCCATCTCAAAA
PRIMER_LEFT_2=363,22
PRIMER_RIGHT_2=647,22
PRIMER_LEFT_2_TM=59.797
PRIMER_RIGHT_2_TM=59.930
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.2996
PRIMER_LEFT_3_PENALTY=0.151106
PRIMER_RIGHT_3_PENALTY=0.148509
PRIMER_LEFT_3_SEQUENCE=GGTAAGTGTTGGGAACGACATT
PRIMER_RIGHT_3_SEQUENCE=CTCAATGACAGACAAGTTCCCA
PRIMER_LEFT_3=346,22
PRIMER_RIGHT_3=526,22
PRIMER_LEFT_3_TM=60.151
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.3087
PRIMER_LEFT_4_PENALTY=0.239177
PRIMER_RIGHT_4_PENALTY=0.069526
PRIMER_LEFT_4_SEQUENCE=ATGGCCGTATAAGCCATAACTG
PRIMER_RIGHT_4_SEQUENCE=ATCTATGGCTCCCATCTCAAAA
PRIMER_LEFT_4=407,22
PRIMER_RIGHT_4=647,22
PRIMER_LEFT_4_TM=60.239
PRIMER_RIGHT_4_TM=59.930
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_03938 Ps_contig_mira-and-tgicl-ass_franssen_31533
SEQUENCE=AGACTCTTAATCTTAAAGTGGACAAAGACAATAATTGAAGATACTTTCATTTCATGAAATAATAACAAGAGAAAACGTGCAAATAACAGTTTCAGTTTAGCTTGGACAGTACTCAAAATTTATAGAAAAATACAAAGACTCTTAAAATATTTACACATACCAAAAGGCATGGTAATTCTAAAGCCAGCTAAATTCAGTGATAATCATTGTATAAGTTAAAATTAACTTAGTCTTCTTTGTCACCTTCTTTATACTCTTTGATTTCTTCACCTTCTGTTGTAGAGCTGCTTGGTTGATTAACCTTATCTTGGTTTGCTACTCTCTGATATTCACCACTATCACCTCCTTTACCTAAGGTTTGAATAAGCATTAGCACTGCAATCATAAAAACAACTATGTACACCATTCTAATGTCAAAAGATCTTTGGAGATATTGCGTCAATGTTGGCCCGCTAAGCCTTTTCTTTACGGTTCTTCCTTCTCTATATCCTTCTAGAAATTTAGTGATTTGAGTTCCTTGATCCTCTTCCTCGATGTTCTCGGAACCAACAACAGAAAGATAATCATCTTCTTTATCCCAAATGACCATTTTTGGTAGTTTAGAACTGGTATCGAATTTATCAGCAAATTCATCGAGCTGTTTAACTCCAACATAACCAAATATTAAGTCACGATTTGCAGATGCAGCACCCCTCAATAACTTAACCAGTTCTTTTGATCTTTCTTCATTTTCATCCTCCACAATAGTCAAAACTATTTTCCTACCATCAGCTTTTATCAACTTTAGTGTCTCGTAGGATACAGGCACAACCAAAGGAATGAGATTTTGTTTTACAAAGTCTTCCAAAAATTCATCTTCAAAGGGGCCATAAAATGTGTTCCGCTCATTGTACTGTTGATTAATGGAAACCAATGCAGGAATCTTATCAAAGTCATATGTTATCATGAGATCCTCTGAAAAATCCTTTGCAACAGAAAACCATGCGTTTTTCTTATACTTTACACCTAACTTTTCTATAACCGAACTGTCCAACCCAAAACCAATAAATACCGGGAAAAAAGTACCAGCTTCTTCAACAAAACTATTCACAGCAGAATCTGAGTCGAGAACAGAAACATCAGAAGCAGCAAATTTCTTAAGATAACGAACAAGTAAATCAGCTTTCCTGGGTCCACGGTATTCTGTAGGGACGCCGTGATTAAACAGCAAAATGGTTGGATAGGCATCAACATCATGTTTTCTTGCAAGACGAGCATGCTTGTCTGCATCTACTTTTGCTATGAGTATGGGTTCTTTCAATTTTGCAAGTACAGGTGCGGCTGCATCTAACTCGGGAGAGAGACGCTTGCAGTGGCCACACCAGGGAGCGTAGAAATCAACCAAGATGTGATCGAAGGATGAAATGGCGGAATCGAAATTGGACTCGTCGAGTATCAACACCTTTCCATCCACAGTTAATGGTTCGGCGGCGCGGCAAGAGTGGATCAATAACACAAGACAAAAAATAACCCAAATCTTCATCGTTGGATCCAAACAGATCCTTCTTCTTCTTTGATTTTGCAGTGGGAGTGTATACTATGAATGAATCACTCTCTCAATCACGGAAGATCTAATTCAACTCCAAATAACTGATATCCCCGC
TARGET=1542,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0842
PRIMER_LEFT_PENALTY=0.073614
PRIMER_RIGHT_PENALTY=0.010545
PRIMER_LEFT_SEQUENCE=CAAGATGTGATCGAAGGATGAA
PRIMER_RIGHT_SEQUENCE=TCTTCCGTGATTGAGAGAGTGA
PRIMER_LEFT=1381,22
PRIMER_RIGHT=1608,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.989
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=0.1778
PRIMER_LEFT_1_PENALTY=0.167242
PRIMER_RIGHT_1_PENALTY=0.010545
PRIMER_LEFT_1_SEQUENCE=AACCAAGATGTGATCGAAGGAT
PRIMER_RIGHT_1_SEQUENCE=TCTTCCGTGATTGAGAGAGTGA
PRIMER_LEFT_1=1378,22
PRIMER_RIGHT_1=1608,22
PRIMER_LEFT_1_TM=59.833
PRIMER_RIGHT_1_TM=59.989
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.3783
PRIMER_LEFT_2_PENALTY=0.073614
PRIMER_RIGHT_2_PENALTY=0.304636
PRIMER_LEFT_2_SEQUENCE=CAAGATGTGATCGAAGGATGAA
PRIMER_RIGHT_2_SEQUENCE=CGGGGATATCAGTTATTTGGAG
PRIMER_LEFT_2=1381,22
PRIMER_RIGHT_2=1640,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.695
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.4719
PRIMER_LEFT_3_PENALTY=0.167242
PRIMER_RIGHT_3_PENALTY=0.304636
PRIMER_LEFT_3_SEQUENCE=AACCAAGATGTGATCGAAGGAT
PRIMER_RIGHT_3_SEQUENCE=CGGGGATATCAGTTATTTGGAG
PRIMER_LEFT_3=1378,22
PRIMER_RIGHT_3=1640,22
PRIMER_LEFT_3_TM=59.833
PRIMER_RIGHT_3_TM=59.695
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=0.5320
PRIMER_LEFT_4_PENALTY=0.227356
PRIMER_RIGHT_4_PENALTY=0.304636
PRIMER_LEFT_4_SEQUENCE=TATCAACACCTTTCCATCCACA
PRIMER_RIGHT_4_SEQUENCE=CGGGGATATCAGTTATTTGGAG
PRIMER_LEFT_4=1432,22
PRIMER_RIGHT_4=1640,22
PRIMER_LEFT_4_TM=60.227
PRIMER_RIGHT_4_TM=59.695
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_03939 Ps_contig_mira-and-tgicl-ass_franssen_31534
SEQUENCE=CTTGTAGATCAATAACTGAATCACTCACCTTCCGTCGGGGGTCTCAAATTTCAGGGAAGAGACTCGAGAGAAGAAATAGAGAGCTCATCCTAGCATAACAAAAAGAAACAAAATGTTGTCATCAGGTTGCGTGCTGTCTTCAACTTCAACTGTATCTGCACGAACTGCCTTCTCTCCCCTCAACTGCCCGCCAAGAGATCCATCCTTCTCTTCATCATCGTTTTCTTTCTCTCACCGCACTTTCAATCCCCTATGCCTCAGATCCTTCACTCGCCATTACGGAATTCGAGTTGTCGACAAATCGAAAACCAGCAATGGAAGAATCACAGGGTTTCGCGTCCGTTCATATGCTCCTCCTTATCCTCTTACTACTCCTAATGTCCAGTGGATCTCTACTGTTTCTGCTCTGGTTTTAGTACTGGCAAAGGGCACCACTGTGCCCAAATCATTTCTTGTTCCATTATTTGCTTTGCAAGCACCTCCAGCTGTCTTTGCCTGGATCAAAGGTAGATATGGTGTGTGGTCAGCATTCTTGGCACTTCTGGTCCGTCTCTTCTTTCATATTCCTGGTGAACTTGAGTTGCCATTTATAGCGTTACTATTGGTGATTGTAGCTCCTGACGAGGCTATAAGATTAAGGCACACAAAAGAAGGTGCTGCAATTTCTTTATTGATTGCAGCTTATTTGGCTTTCCAGCATTTCTCAAGAACAAGCTTGGACAAATCATTTGACCAAGGTTCAGTTGTTGCCACCTTAGCTGTCATCGGCATCACTGTTGCGTCTGTGTTGCTTCTTATCTGAGTTGGGTTTGAAATGATTTATGCTCATAGTTACATTGTAAATAAGAATAATACCTGTTGAAATGTTGATATTGTTCGGATATATATATATATATTATGTAACATGTTTGACACTCAAATCCTTTACTTTCTACTTGCTAGTTTAAATAATGGAGTGTAAAAAGTTTGTAA
TARGET=881,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03940 Ps_contig_mira-and-tgicl-ass_franssen_31539
SEQUENCE=AGGGAAATTCGGATAAAAGGCCAATAGAAGAAAACCAAACGACAGTTTCTCTCTCTGAATTGTTTGAATTGAAAGAAAGAAAAAATAGAGAGAGAAAGAAAGAAAGAAATGGAAGCTGCGATGGGACTGATGCGGAGGATTCCACCCAAACACACCGAAACAGCTCTCTCTGCGCTTCTCAGTCTTATGCCCAACAACTCTTCCGATCTCCTCTCTCAAGTCGATCAACCACTCCAGGTTTTGTGCGATGTGGACTGTGGGAAGGAGTTCATTTTGTGTGAATATAACAGAGATGCAGACTCTTACAGATCACCCTGGTCGAATAAATACCATCCACCATTAGAAGATGGATCGCTTCCTTCTTCAGAGTTGAGGAAGCTTGAAATTGAAGCAAATGAAATATTTGCTATATATCGTGACCAGTATTATGAAGGTGGCATTTCATCAGTTTATATGTGGGAAGATGAAAATGAAGGTTTCGTAGCTTGCTTCTTGATAAAGAAAGATGGCTCAAAGACAGGGCAAGGTAGACGAGGATATTTAGAGGAAGGAGCATGGGATGCCATACATGTTATAGAGGTTGGGCCAGAGGAAGAAGAAAACACCAATTACCGGTTAACCAGTACAGTCATGTTGACTTTAACTACAAACAACGAGTCATCGGGAACCTTCAGTTTGTCTGGATCAATTAGACGTCAGATGAGTATGAAGCTATCAGTTGCTGATGGACATCTTTGTAACATGGGAAGGATGATTGAAGAAATGGAGAGTAAGCTGAGGAACTCACTTGATCAGGTATACTTTGGGAAAACAAGAGAAATGGTTTGCACACTAAGACCACCATCTGAAGTGGTGCAGTTGAGAATCCCTGAGAGCTGATCGCGTTAAGCCGTGTGCCGGAGACAATTTTAGTTTGTTTTCTTCATATGTTTTAGCATATTGGCTGGTAATTTGTGTTACAGTATTTTAAAATAGGCAGTTCACATGGATTGGTGATTCTTCTGATGGCGTTTGTCTTACTGAGGTAGTTCTGCTTGTTGTTTGATGATTGTAAACTGTTGTGTTCTGTAGTGGATAGCAGTATAGCACCATGTTTGTTGTTTTCTTGTTGATTCAACAGAACAGTGTAACTCAAAAATAGCTTATGGGAATTCCTGAACCTAAGCAATTTATGGTGGATTATTATTTTATTTTTTGTTTYYATGTCACATCAAAAAATTTATAATGGTTGAGTTCTTGAAT
TARGET=71,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03941 Ps_contig_mira-and-tgicl-ass_franssen_31539
SEQUENCE=AGGGAAATTCGGATAAAAGGCCAATAGAAGAAAACCAAACGACAGTTTCTCTCTCTGAATTGTTTGAATTGAAAGAAAGAAAAAATAGAGAGAGAAAGAAAGAAAGAAATGGAAGCTGCGATGGGACTGATGCGGAGGATTCCACCCAAACACACCGAAACAGCTCTCTCTGCGCTTCTCAGTCTTATGCCCAACAACTCTTCCGATCTCCTCTCTCAAGTCGATCAACCACTCCAGGTTTTGTGCGATGTGGACTGTGGGAAGGAGTTCATTTTGTGTGAATATAACAGAGATGCAGACTCTTACAGATCACCCTGGTCGAATAAATACCATCCACCATTAGAAGATGGATCGCTTCCTTCTTCAGAGTTGAGGAAGCTTGAAATTGAAGCAAATGAAATATTTGCTATATATCGTGACCAGTATTATGAAGGTGGCATTTCATCAGTTTATATGTGGGAAGATGAAAATGAAGGTTTCGTAGCTTGCTTCTTGATAAAGAAAGATGGCTCAAAGACAGGGCAAGGTAGACGAGGATATTTAGAGGAAGGAGCATGGGATGCCATACATGTTATAGAGGTTGGGCCAGAGGAAGAAGAAAACACCAATTACCGGTTAACCAGTACAGTCATGTTGACTTTAACTACAAACAACGAGTCATCGGGAACCTTCAGTTTGTCTGGATCAATTAGACGTCAGATGAGTATGAAGCTATCAGTTGCTGATGGACATCTTTGTAACATGGGAAGGATGATTGAAGAAATGGAGAGTAAGCTGAGGAACTCACTTGATCAGGTATACTTTGGGAAAACAAGAGAAATGGTTTGCACACTAAGACCACCATCTGAAGTGGTGCAGTTGAGAATCCCTGAGAGCTGATCGCGTTAAGCCGTGTGCCGGAGACAATTTTAGTTTGTTTTCTTCATATGTTTTAGCATATTGGCTGGTAATTTGTGTTACAGTATTTTAAAATAGGCAGTTCACATGGATTGGTGATTCTTCTGATGGCGTTTGTCTTACTGAGGTAGTTCTGCTTGTTGTTTGATGATTGTAAACTGTTGTGTTCTGTAGTGGATAGCAGTATAGCACCATGTTTGTTGTTTTCTTGTTGATTCAACAGAACAGTGTAACTCAAAAATAGCTTATGGGAATTCCTGAACCTAAGCAATTTATGGTGGATTATTATTTTATTTTTTGTTTYYATGTCACATCAAAAAATTTATAATGGTTGAGTTCTTGAAT
TARGET=93,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03942 Ps_contig_mira-and-tgicl-ass_franssen_31543
SEQUENCE=CGTTTTTTCTTTTTCCNTAATNAAAATACTTAATTAAATTTAATTGTATGAGAATCTCTCATTCTCATATTATAAGCAATTTTATTAGTATTCAATTTTAATTTGAAAGAACATCCAATAGCAGTACAAATATCTGAAAGTTTGTTTTCTATATTGCAAATAAATAAATGAATAATTGAGCTCCAAGAAAATCTTGAAATTTAGGGGGTCCAATTTTGAAGTGCTTGCTGCATTAAGACTGCTGTGATACTTCCTATTGATATGTTGATCACATTGACTAAATCATTATTCAGCCATTCAAATCCTTTCTTATCTTGAAGTAAAGCACCAATTAGGCTTTCACCAAGATTAGCAAACTGGGAAGCTAATAAACATATAACTACTTCATGTACACCAATCTCGCCCAGGAGAAAACTGAGAAAGGCAAGAAGTAGAGCTGCTAAAATTCCAGCCAAGGTTCCTTCAACACTAACTGCCCCTTCCGTGCCTCTTGGAACTATCTTGAATGTCGTAACTAGGTACGTTGTTTTTCCATATGCCTTGCCTATCTCACTTGAAACTGTGTCGCTCAACTTGGTACAGAAACTAGCAACAAACCCAAGTCTCCAAAGCTGAGAAAATGCTGCACCTCCAACTCCAGAAATAGCCATGAGAGAACAAATGCAACCCGCAGCACTGGATCCAACAACACTCTCGGGACCCCTTCTTCCTCTCTTTTTCTCAGCAACTCCTTCTTCCATTTTCTGTGTAATTTTGACTTTTGTTGCTGCTGTACCAATGATAAAAAAGGCGGCAACAAGTAGAAACCCAGGAGACCCAAAAGCACGCCAAGTAAGCGTACCAAGCAAAAAAGCAGCAACAATCCCAGAAAACGACAAACCAGTAACCAAAATTGGAGAACCCAACAGAAAAATCAAGAGGTTAGCCAGCAAAGAGGATTCCCAGGTGGCAGGTGACGATTGAACAATTGCCACAGCTCCTCCAAAAGCATCTTGAAAACTAGAAACACGCAAAGAATGCATGTTTTTGGGGATTCGGTTGTGAAGAACAAGAGGGTTTGGAAATCGAGTTATTAAATTGGGTTTTGGAGAGAGAGAAATGGGTTTGCGTGGGAAGTGAAAAGGAAGAGAAAGATACGTGATCGAGTGAAGAGTTAATTGCGCCATAATGTGTCTGTCGATGTCAACGTGAAGGGAAAAAAGGAATGAGAATGAAAGAAAACTCTGCGTTGATACACTGCTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03943 Ps_contig_mira-and-tgicl-ass_franssen_31543
SEQUENCE=CGTTTTTTCTTTTTCCNTAATNAAAATACTTAATTAAATTTAATTGTATGAGAATCTCTCATTCTCATATTATAAGCAATTTTATTAGTATTCAATTTTAATTTGAAAGAACATCCAATAGCAGTACAAATATCTGAAAGTTTGTTTTCTATATTGCAAATAAATAAATGAATAATTGAGCTCCAAGAAAATCTTGAAATTTAGGGGGTCCAATTTTGAAGTGCTTGCTGCATTAAGACTGCTGTGATACTTCCTATTGATATGTTGATCACATTGACTAAATCATTATTCAGCCATTCAAATCCTTTCTTATCTTGAAGTAAAGCACCAATTAGGCTTTCACCAAGATTAGCAAACTGGGAAGCTAATAAACATATAACTACTTCATGTACACCAATCTCGCCCAGGAGAAAACTGAGAAAGGCAAGAAGTAGAGCTGCTAAAATTCCAGCCAAGGTTCCTTCAACACTAACTGCCCCTTCCGTGCCTCTTGGAACTATCTTGAATGTCGTAACTAGGTACGTTGTTTTTCCATATGCCTTGCCTATCTCACTTGAAACTGTGTCGCTCAACTTGGTACAGAAACTAGCAACAAACCCAAGTCTCCAAAGCTGAGAAAATGCTGCACCTCCAACTCCAGAAATAGCCATGAGAGAACAAATGCAACCCGCAGCACTGGATCCAACAACACTCTCGGGACCCCTTCTTCCTCTCTTTTTCTCAGCAACTCCTTCTTCCATTTTCTGTGTAATTTTGACTTTTGTTGCTGCTGTACCAATGATAAAAAAGGCGGCAACAAGTAGAAACCCAGGAGACCCAAAAGCACGCCAAGTAAGCGTACCAAGCAAAAAAGCAGCAACAATCCCAGAAAACGACAAACCAGTAACCAAAATTGGAGAACCCAACAGAAAAATCAAGAGGTTAGCCAGCAAAGAGGATTCCCAGGTGGCAGGTGACGATTGAACAATTGCCACAGCTCCTCCAAAAGCATCTTGAAAACTAGAAACACGCAAAGAATGCATGTTTTTGGGGATTCGGTTGTGAAGAACAAGAGGGTTTGGAAATCGAGTTATTAAATTGGGTTTTGGAGAGAGAGAAATGGGTTTGCGTGGGAAGTGAAAAGGAAGAGAAAGATACGTGATCGAGTGAAGAGTTAATTGCGCCATAATGTGTCTGTCGATGTCAACGTGAAGGGAAAAAAGGAATGAGAATGAAAGAAAACTCTGCGTTGATACACTGCTT
TARGET=1088,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1986
PRIMER_LEFT_PENALTY=0.087031
PRIMER_RIGHT_PENALTY=0.111534
PRIMER_LEFT_SEQUENCE=ATCAAGAGGTTAGCCAGCAAAG
PRIMER_RIGHT_SEQUENCE=ACATCGACAGACACATTATGGC
PRIMER_LEFT=912,22
PRIMER_RIGHT=1182,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=59.888
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.2205
PRIMER_LEFT_1_PENALTY=0.108929
PRIMER_RIGHT_1_PENALTY=0.111534
PRIMER_LEFT_1_SEQUENCE=CCTCCAAAAGCATCTTGAAAAC
PRIMER_RIGHT_1_SEQUENCE=ACATCGACAGACACATTATGGC
PRIMER_LEFT_1=978,22
PRIMER_RIGHT_1=1182,22
PRIMER_LEFT_1_TM=60.109
PRIMER_RIGHT_1_TM=59.888
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.2432
PRIMER_LEFT_2_PENALTY=0.087031
PRIMER_RIGHT_2_PENALTY=0.156135
PRIMER_LEFT_2_SEQUENCE=ATCAAGAGGTTAGCCAGCAAAG
PRIMER_RIGHT_2_SEQUENCE=ATGGCGCAATTAACTCTTCACT
PRIMER_LEFT_2=912,22
PRIMER_RIGHT_2=1165,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=60.156
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=0.2448
PRIMER_LEFT_3_PENALTY=0.088662
PRIMER_RIGHT_3_PENALTY=0.156135
PRIMER_LEFT_3_SEQUENCE=GTAACCAAAATTGGAGAACCCA
PRIMER_RIGHT_3_SEQUENCE=ATGGCGCAATTAACTCTTCACT
PRIMER_LEFT_3=882,22
PRIMER_RIGHT_3=1165,22
PRIMER_LEFT_3_TM=60.089
PRIMER_RIGHT_3_TM=60.156
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.2651
PRIMER_LEFT_4_PENALTY=0.108929
PRIMER_RIGHT_4_PENALTY=0.156135
PRIMER_LEFT_4_SEQUENCE=CCTCCAAAAGCATCTTGAAAAC
PRIMER_RIGHT_4_SEQUENCE=ATGGCGCAATTAACTCTTCACT
PRIMER_LEFT_4=978,22
PRIMER_RIGHT_4=1165,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=60.156
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_03944 Ps_contig_mira-and-tgicl-ass_franssen_31545
SEQUENCE=CCTTTAAACGTTAATTTACTAACCAACTAGTCCCACCCAAAACGAACACAACTNGAGTATAAAGTTACAAAACTTGATTTATTCAATAGCAGAATATCAAGGATACTTATTTCTCAAATCAAACCTTTTCTAAGGCTTCCAACTGAAATCTTAATGACCATAGACAATAAATTATTACAACAATGAATAAGCCTTGTACAGAGAAGTGTTGGATCTATTTGTGAGATAACAGGAGAGTTCAAGAAATGAAGTACATAAAATAATAATAATAGGCCACCCGACCGAAATAGAACTAGACCTGCAAGAAGCTTGCATACTTTGCACCCAAGAAAAGCCTTTGGATGCAGAGAAGTGCATTTTTGGTAACCTCAGCACTCTCATGATTCATTAGTTTCATCACTCGTTCCTTGGCTTTAAGGTCTGTCACAATGATCCGCCCAGCCGGATGGCATTGGATGAACTGTGAAATGTCAAAGCAAGCAACAGCTAGAGTCCTTGGATCATTGGACGAATCCAAAATTGTTAATAGGACCCTTAGAATCTGGAAATCATGATCTTCAAAATTGGTTATATTTTCTCGCCAAAATAAGGGATCTTTGTGCATGGGAGACCAGTCTAGATTGCCAAGGAGAACTTCTTGCTTGTACTTATCAAAAGAACTAAGCTTCTTGATGTTATCCTTAAGCCCTTCTTCAAGGGAATTCAGTGCCTCCAACAAGTCCTCATCACTCCACGCTTGTGCTTTCAAACTTTGAACAACCTGTGGAAGCTGAAGATCAACCATCTGAGCACCCAATGTCCCTTTCGACAACAAGTTTTTGAGTGTCAAGACAACAACTCTAACAACCTTTTCTTTTGTAGAACTCTTAACAACCTCAATAAGTCTTGGAAGGGTCCTTGAAGTAGCCAAATATTCAATTGCAGGATCATAATATGATAAGAGCCATATGCAGAGACATGTTTCATAAAGAAGCTGAATAGATTGTTGAGTGGATGCTGGACAAATCAATGGCACAAGCAACTTGACCCCATCGGCTTGAACAAAGGAAGACCTAACAACAGGTTCCTTAAGTAGACTTGATAAGCAATTGATAGCTGTTGGAACTCCACGAGTAGGATGAGAAGGTTTCTTCAGCTGCTCACAAAGCCATTTTACCAATCCTATCAAAACATCATCAATAGAGGTAATTGATTTCTTTTCATTTGATGCTTCTCCCTTTGAAGCATTGCCACTCTGATTTTTTGGCCTGACACTGACTATTAAAGCAAGGATCTTACAACTTTTCTCTTGTACGAACCAATTTCCTTTCCGAAGCAATCTTAGGAAAGGCTCATAAGTGTCATCATCCGCAAGGGCATTGTCATGAAACAATCTTGCTCTTTTCGGGTTCGCTGTCAGCATTTCATCTATCAGAGCCAAAACATACTCAACTGTATCCTCTTTGAAAATATCACGCAAAACATGAACAAACACGCGAACATAAGCCGGACCATCATCATCGAGCAACTGTGCTCTCTGACTTTCCGATCGGTGATCATAGCGCCTTAAGAGCTGAAGGCTAGTACCAGAGATGAGCTTGGTGGACATGTACGTCTCCCATGGAATGTCCCTACTCAGAACCTGATCGGTGGTTAATTCAGCCTGGTCCATGATAGTGGAGAACCCTAGAATTCAAAGAGTTCGCCGGAGAAACTGTTACGAATTGGTACTCTTGTACCTTGCGCAAGCGATTGGGTGGTTTTTCGATTTGAAGCCGAGAAAAACACAACTAGAAAGAAAATTAAAATAGGTCAGTTTTGGAAGCAAATNNYCGAC
TARGET=259,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03945 Ps_contig_mira-and-tgicl-ass_franssen_31548
SEQUENCE=GTCGGGGTAAAGGAGGAAGGAATTGAATGTTGTTAAATTTAGGTTGGTAGGAAGGAGTAGTGATTTTGACCGGCAAGTGAGGTTGAATTATTATATAGTTGACACCATCTCCTCCCACGTTGTTTCGTCTATCTTCTTCTTCGTGAATCCCTTCCAAAGTAAAAGTATGAGTTCCGAGGGCGTCGGGGAAGATTGCCTTGCTTGGGCGGCAAGAGATGCATCTGGAGTTTTGTCGCCTTACAAATTCAATCGCAGGGCTCTTGGGAGTGAAGATGTTTATGTCAAAATTACACACTGTGGCGTTTGTTATGCTGATGTAATTTGGACAAGGAATAAACACAGGGATTCAAAGTATCCTGTTGTGCCGGGACATGAAATTGCTGGGGTTGTAGCAAAGGTTGGTCCTAATGTCCAACGTTTCAAGGTTGGTGACCATGTTGGAGTGGGGACTTACGTCAACTCATGCAGGGAGTGCGAGTATTGTAACGATAGACTAGAAGTTCATTGTGTCAAGGGATCGGTTTTCACATTTAATGGTGTTGATTTTGATGGTACTGTTACAAAAGGAGGATACTCCACTTCCATCGTAGTACATGAAAGGTATTGCTTCTTGATACCTAAAAGCTACCCTTTAGCTTCAGCAGGTCCTTTGCTTTGTGCTGGCATCACAGTTTATTCTCCCATGATGCGTCATAAGATGAATCAACCTGGTAAGTCTCTTGGAGTGATTGGTCTTGGTGGCCTCGGTCATATGGCGGTAAAATTTGGGAAGGCATTTGGTTTGCGTGTGACGGTTTTCAGCACTAGTGTGTCCAAGAAAGAGGAGGCGCTGAACCGACTTGGCGCGGACAATTTTGTTGTTTCATCTAATCAAGAAGAGATGAAGGCATTGGCTAAATCATTGGATTTTATCGTTGACACGGCATCAGGTGATCATCCGTTTGATCCTTACATGTCACTCTTGAAGGTATCTGGTGTTCTGGTCTTAGTTGGGTTCCCAAGTGAAGTCAAATTCAGCCCTGCAAGCCTTAATTTAGGATCAAAAACTGTTTCCGGAAGTATTACAGGTGGTACTAAAGAGATACAGGAGATGATTGACTTCTGTGCTGCAAACGGGGTTCACCCAGATATAGAACTGATTCCAATTGGGTACTCGAACGAAGCTCTTGAAAGAGTCATAAATAAGGACGTGCAATATCGGTTTGTGATAGACATTGGAAACTCTCTTAAGTTGAATTAGAACTAGACAGGGAAGGACTGCCTTGTTTGATATTACATACCAGAAGCAGAGAGAGAGATGTTCAGTGTTCCATAATGATGGCCAGGATGATTCAAATTTCATATATCTAGCACTTGAGAGGGTCATAAACAGGGAAATAAAATACCCGTATGTAGTAGCACTTAGTCTTTTTATTGCTAACAAAAAAATTATGAGTTCGGTTTCGATTTTCAACTTTCGAGAATAAACTAAGGCAGGCTTCAGATGCAACAGCTCAGAGAGCCTTTAAGAAGAAATTATTTAAGATATAGTAATCTAGTAGACNTTGAAGAACTCAAATAAAATAGCATGTTAGCTAA
TARGET=1288,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0820
PRIMER_LEFT_PENALTY=0.073038
PRIMER_RIGHT_PENALTY=0.008930
PRIMER_LEFT_SEQUENCE=GATTGACTTCTGTGCTGCAAAC
PRIMER_RIGHT_SEQUENCE=CCCTGTTTATGACCCTCTCAAG
PRIMER_LEFT=1092,22
PRIMER_RIGHT=1373,22
PRIMER_LEFT_TM=59.927
PRIMER_RIGHT_TM=59.991
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.1481
PRIMER_LEFT_1_PENALTY=0.139129
PRIMER_RIGHT_1_PENALTY=0.008930
PRIMER_LEFT_1_SEQUENCE=CGGTTTGTGATAGACATTGGAA
PRIMER_RIGHT_1_SEQUENCE=CCCTGTTTATGACCCTCTCAAG
PRIMER_LEFT_1=1198,22
PRIMER_RIGHT_1=1373,22
PRIMER_LEFT_1_TM=59.861
PRIMER_RIGHT_1_TM=59.991
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=0.1525
PRIMER_LEFT_2_PENALTY=0.139129
PRIMER_RIGHT_2_PENALTY=0.013401
PRIMER_LEFT_2_SEQUENCE=CGGTTTGTGATAGACATTGGAA
PRIMER_RIGHT_2_SEQUENCE=GTTGAAAATCGAAACCGAACTC
PRIMER_LEFT_2=1198,22
PRIMER_RIGHT_2=1453,22
PRIMER_LEFT_2_TM=59.861
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.2262
PRIMER_LEFT_3_PENALTY=0.139129
PRIMER_RIGHT_3_PENALTY=0.087031
PRIMER_LEFT_3_SEQUENCE=CGGTTTGTGATAGACATTGGAA
PRIMER_RIGHT_3_SEQUENCE=CATCTGAAGCCTGCCTTAGTTT
PRIMER_LEFT_3=1198,22
PRIMER_RIGHT_3=1485,22
PRIMER_LEFT_3_TM=59.861
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.2288
PRIMER_LEFT_4_PENALTY=0.219844
PRIMER_RIGHT_4_PENALTY=0.008930
PRIMER_LEFT_4_SEQUENCE=AAATAAGGACGTGCAATATCGG
PRIMER_RIGHT_4_SEQUENCE=CCCTGTTTATGACCCTCTCAAG
PRIMER_LEFT_4=1179,22
PRIMER_RIGHT_4=1373,22
PRIMER_LEFT_4_TM=60.220
PRIMER_RIGHT_4_TM=59.991
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.8000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_03946 Ps_contig_mira-and-tgicl-ass_franssen_31554
SEQUENCE=TACGCGGGGAGGATAGAGAGNGAAGAAAAAGGGTACATNAGATTTGTGTTTTCTGTAGTTTTTTTCTTCATTTCACGTCATTTTTCTCTTCTCTTCTCTTCCATTTCTTGTTGTTGTTCTTTGCAGGGAACTGAATCAGGGTAGAGGGAAGAGAGAGAGAGAGAGAAAAGAAGCTTTCTCTCTCTCGTTTTTGTGTTTCTCTCTTTTGTTTTTCATAAAAAAACTGAGGTTTGATTTGTGGGTATTTTTAGTGGGTTATTGAGTTGGGTTGTTTGGGTTGGAATAAACAAGTATGAATATGATGAGAAGGTTGAAAAGCATTGCTTCTGGGAGAACCTCTATTTCATCAGATCCTGGAGGTGATTCTACTACAAAAAGAGCCAAGTTCGATCAAGGGACTGAAGGAAAAGAAAACGAGGAAACAAATCGTGTTGAGGGAAGTGAAAATAATCGGGATAAGTATGTAGATGCGTCACAAGAAGATACGTCTAGTAAATGTATTACTACTGTTTCTGCGGTTTCTAAAACTGAGAAGTCGGGTTATGATCAGCTACCAAAAGAATTACTTGAAATGAAAATTAAAGATGATAAAAGCAAAAACAACAAAGAAAAGGACATGGAGGCAACTGTTGTGAACGGAAACGGAACGGAAACCGGTCAAATAATTACAACTTCTATTGGTGGTCGAGACGGACAACCAAAGCGGATAATCTCATACATGGCAGAACGTGTAGTTGGTACCGGTTCTTTTGGTGTTGTTTATCAGGCTAAGTGTGTTGAAACTGGTGAAGCCGTTGCGATTAAGAAAGTCTTACAAGACAAGAGATACAAAAATAGGGAACTCCAAGTTATGCGTATGCTTGAACATACTAATGTTCTTAAACTAAAGCATTGTTTCTACTCGACGGCTGAAAAAGAAGAGGTGTACCTTAACTTAGTGTTGGAGTATGTACCTGAAACTGTGTACAGAGTTTCAAAGCACTATGTCAGGATGCACCAACACATGCCTATACTCTACGTCCAACTATATACTTATCAGATATGCCGCGGTTTAAATTACATGCACCATGTGGTTGGAGTATGTCACCGTGACATCAAACCTCAAAATCTATTGGTTAATCCGACGAGT
TARGET=84,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4711
PRIMER_LEFT_PENALTY=0.452261
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=GGGGAGGATAGAGAGNGAAGAA
PRIMER_RIGHT_SEQUENCE=ACAACCCAACTCAATAACCCAC
PRIMER_LEFT=5,22
PRIMER_RIGHT=271,22
PRIMER_LEFT_TM=60.452
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.5962
PRIMER_LEFT_1_PENALTY=0.452261
PRIMER_RIGHT_1_PENALTY=0.143968
PRIMER_LEFT_1_SEQUENCE=GGGGAGGATAGAGAGNGAAGAA
PRIMER_RIGHT_1_SEQUENCE=CAACCCAACTCAATAACCCACT
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=270,22
PRIMER_LEFT_1_TM=60.452
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.6792
PRIMER_LEFT_2_PENALTY=0.452261
PRIMER_RIGHT_2_PENALTY=0.226971
PRIMER_LEFT_2_SEQUENCE=GGGGAGGATAGAGAGNGAAGAA
PRIMER_RIGHT_2_SEQUENCE=ACCCACAAATCAAACCTCAGTT
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=242,22
PRIMER_LEFT_2_TM=60.452
PRIMER_RIGHT_2_TM=59.773
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.7701
PRIMER_LEFT_3_PENALTY=0.452261
PRIMER_RIGHT_3_PENALTY=0.317850
PRIMER_LEFT_3_SEQUENCE=GGGGAGGATAGAGAGNGAAGAA
PRIMER_RIGHT_3_SEQUENCE=CCTGCAAAGAACAACAACAAGA
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=126,22
PRIMER_LEFT_3_TM=60.452
PRIMER_RIGHT_3_TM=60.318
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=0.8762
PRIMER_LEFT_4_PENALTY=0.452261
PRIMER_RIGHT_4_PENALTY=0.423908
PRIMER_LEFT_4_SEQUENCE=GGGGAGGATAGAGAGNGAAGAA
PRIMER_RIGHT_4_SEQUENCE=TCCCTCTACCCTGATTCAGTTC
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=148,22
PRIMER_LEFT_4_TM=60.452
PRIMER_RIGHT_4_TM=59.576
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_03947 Ps_contig_mira-and-tgicl-ass_franssen_31554
SEQUENCE=TACGCGGGGAGGATAGAGAGNGAAGAAAAAGGGTACATNAGATTTGTGTTTTCTGTAGTTTTTTTCTTCATTTCACGTCATTTTTCTCTTCTCTTCTCTTCCATTTCTTGTTGTTGTTCTTTGCAGGGAACTGAATCAGGGTAGAGGGAAGAGAGAGAGAGAGAGAAAAGAAGCTTTCTCTCTCTCGTTTTTGTGTTTCTCTCTTTTGTTTTTCATAAAAAAACTGAGGTTTGATTTGTGGGTATTTTTAGTGGGTTATTGAGTTGGGTTGTTTGGGTTGGAATAAACAAGTATGAATATGATGAGAAGGTTGAAAAGCATTGCTTCTGGGAGAACCTCTATTTCATCAGATCCTGGAGGTGATTCTACTACAAAAAGAGCCAAGTTCGATCAAGGGACTGAAGGAAAAGAAAACGAGGAAACAAATCGTGTTGAGGGAAGTGAAAATAATCGGGATAAGTATGTAGATGCGTCACAAGAAGATACGTCTAGTAAATGTATTACTACTGTTTCTGCGGTTTCTAAAACTGAGAAGTCGGGTTATGATCAGCTACCAAAAGAATTACTTGAAATGAAAATTAAAGATGATAAAAGCAAAAACAACAAAGAAAAGGACATGGAGGCAACTGTTGTGAACGGAAACGGAACGGAAACCGGTCAAATAATTACAACTTCTATTGGTGGTCGAGACGGACAACCAAAGCGGATAATCTCATACATGGCAGAACGTGTAGTTGGTACCGGTTCTTTTGGTGTTGTTTATCAGGCTAAGTGTGTTGAAACTGGTGAAGCCGTTGCGATTAAGAAAGTCTTACAAGACAAGAGATACAAAAATAGGGAACTCCAAGTTATGCGTATGCTTGAACATACTAATGTTCTTAAACTAAAGCATTGTTTCTACTCGACGGCTGAAAAAGAAGAGGTGTACCTTAACTTAGTGTTGGAGTATGTACCTGAAACTGTGTACAGAGTTTCAAAGCACTATGTCAGGATGCACCAACACATGCCTATACTCTACGTCCAACTATATACTTATCAGATATGCCGCGGTTTAAATTACATGCACCATGTGGTTGGAGTATGTCACCGTGACATCAAACCTCAAAATCTATTGGTTAATCCGACGAGT
TARGET=150,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1354
PRIMER_LEFT_PENALTY=0.116582
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=GTTCTTTGCAGGGAACTGAATC
PRIMER_RIGHT_SEQUENCE=ACAACCCAACTCAATAACCCAC
PRIMER_LEFT=115,22
PRIMER_RIGHT=271,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.2183
PRIMER_LEFT_1_PENALTY=0.116582
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=GTTCTTTGCAGGGAACTGAATC
PRIMER_RIGHT_1_SEQUENCE=AGCAATGCTTTTCAACCTTCTC
PRIMER_LEFT_1=115,22
PRIMER_RIGHT_1=324,22
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.2243
PRIMER_LEFT_2_PENALTY=0.116582
PRIMER_RIGHT_2_PENALTY=0.107763
PRIMER_LEFT_2_SEQUENCE=GTTCTTTGCAGGGAACTGAATC
PRIMER_RIGHT_2_SEQUENCE=CTTGATCGAACTTGGCTCTTTT
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=394,22
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.2605
PRIMER_LEFT_3_PENALTY=0.116582
PRIMER_RIGHT_3_PENALTY=0.143968
PRIMER_LEFT_3_SEQUENCE=GTTCTTTGCAGGGAACTGAATC
PRIMER_RIGHT_3_SEQUENCE=CAACCCAACTCAATAACCCACT
PRIMER_LEFT_3=115,22
PRIMER_RIGHT_3=270,22
PRIMER_LEFT_3_TM=60.117
PRIMER_RIGHT_3_TM=60.144
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.3367
PRIMER_LEFT_4_PENALTY=0.317850
PRIMER_RIGHT_4_PENALTY=0.018835
PRIMER_LEFT_4_SEQUENCE=TCTTGTTGTTGTTCTTTGCAGG
PRIMER_RIGHT_4_SEQUENCE=ACAACCCAACTCAATAACCCAC
PRIMER_LEFT_4=105,22
PRIMER_RIGHT_4=271,22
PRIMER_LEFT_4_TM=60.318
PRIMER_RIGHT_4_TM=60.019
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_03948 Ps_contig_mira-and-tgicl-ass_franssen_31554
SEQUENCE=TACGCGGGGAGGATAGAGAGNGAAGAAAAAGGGTACATNAGATTTGTGTTTTCTGTAGTTTTTTTCTTCATTTCACGTCATTTTTCTCTTCTCTTCTCTTCCATTTCTTGTTGTTGTTCTTTGCAGGGAACTGAATCAGGGTAGAGGGAAGAGAGAGAGAGAGAGAAAAGAAGCTTTCTCTCTCTCGTTTTTGTGTTTCTCTCTTTTGTTTTTCATAAAAAAACTGAGGTTTGATTTGTGGGTATTTTTAGTGGGTTATTGAGTTGGGTTGTTTGGGTTGGAATAAACAAGTATGAATATGATGAGAAGGTTGAAAAGCATTGCTTCTGGGAGAACCTCTATTTCATCAGATCCTGGAGGTGATTCTACTACAAAAAGAGCCAAGTTCGATCAAGGGACTGAAGGAAAAGAAAACGAGGAAACAAATCGTGTTGAGGGAAGTGAAAATAATCGGGATAAGTATGTAGATGCGTCACAAGAAGATACGTCTAGTAAATGTATTACTACTGTTTCTGCGGTTTCTAAAACTGAGAAGTCGGGTTATGATCAGCTACCAAAAGAATTACTTGAAATGAAAATTAAAGATGATAAAAGCAAAAACAACAAAGAAAAGGACATGGAGGCAACTGTTGTGAACGGAAACGGAACGGAAACCGGTCAAATAATTACAACTTCTATTGGTGGTCGAGACGGACAACCAAAGCGGATAATCTCATACATGGCAGAACGTGTAGTTGGTACCGGTTCTTTTGGTGTTGTTTATCAGGCTAAGTGTGTTGAAACTGGTGAAGCCGTTGCGATTAAGAAAGTCTTACAAGACAAGAGATACAAAAATAGGGAACTCCAAGTTATGCGTATGCTTGAACATACTAATGTTCTTAAACTAAAGCATTGTTTCTACTCGACGGCTGAAAAAGAAGAGGTGTACCTTAACTTAGTGTTGGAGTATGTACCTGAAACTGTGTACAGAGTTTCAAAGCACTATGTCAGGATGCACCAACACATGCCTATACTCTACGTCCAACTATATACTTATCAGATATGCCGCGGTTTAAATTACATGCACCATGTGGTTGGAGTATGTCACCGTGACATCAAACCTCAAAATCTATTGGTTAATCCGACGAGT
TARGET=177,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1354
PRIMER_LEFT_PENALTY=0.116582
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=GTTCTTTGCAGGGAACTGAATC
PRIMER_RIGHT_SEQUENCE=ACAACCCAACTCAATAACCCAC
PRIMER_LEFT=115,22
PRIMER_RIGHT=271,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.2183
PRIMER_LEFT_1_PENALTY=0.116582
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=GTTCTTTGCAGGGAACTGAATC
PRIMER_RIGHT_1_SEQUENCE=AGCAATGCTTTTCAACCTTCTC
PRIMER_LEFT_1=115,22
PRIMER_RIGHT_1=324,22
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.2243
PRIMER_LEFT_2_PENALTY=0.116582
PRIMER_RIGHT_2_PENALTY=0.107763
PRIMER_LEFT_2_SEQUENCE=GTTCTTTGCAGGGAACTGAATC
PRIMER_RIGHT_2_SEQUENCE=CTTGATCGAACTTGGCTCTTTT
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=394,22
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.2605
PRIMER_LEFT_3_PENALTY=0.116582
PRIMER_RIGHT_3_PENALTY=0.143968
PRIMER_LEFT_3_SEQUENCE=GTTCTTTGCAGGGAACTGAATC
PRIMER_RIGHT_3_SEQUENCE=CAACCCAACTCAATAACCCACT
PRIMER_LEFT_3=115,22
PRIMER_RIGHT_3=270,22
PRIMER_LEFT_3_TM=60.117
PRIMER_RIGHT_3_TM=60.144
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.3117
PRIMER_LEFT_4_PENALTY=0.292834
PRIMER_RIGHT_4_PENALTY=0.018835
PRIMER_LEFT_4_SEQUENCE=TGAATCAGGGTAGAGGGAAGAG
PRIMER_RIGHT_4_SEQUENCE=ACAACCCAACTCAATAACCCAC
PRIMER_LEFT_4=131,22
PRIMER_RIGHT_4=271,22
PRIMER_LEFT_4_TM=59.707
PRIMER_RIGHT_4_TM=60.019
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_03949 Ps_contig_mira-and-tgicl-ass_franssen_31555
SEQUENCE=AAACYTTNNNAGTCCAAAAATCACATAAACCATGTTCATTTCAAACCAATACTTAAACCATGTTCATTCCATTACAACCATGACCAAATGACTTAACATATACATAACCAAGACCAAATTATAAATACACACATACATGATATTACAACACACACAAAATCATGCCAAACAACCATGAACAACACTTAAGACCTATACAAACAGTCCTAAGGCAAGCAAGACATAACCAAGCTCATTTTAAGTCTAGCCTGGCATTGAGCCTTAAGCAGGACCTAACCATAAACCTACACCATTATCCTTTTAAAAAAATAAGCCAGGGCAAATCCAAAAGCCTAGCACCCTGTAACATTAGCACACAAACTCTACAAAAAATCCAACAACATAACCTGCCAAGCCTAGACCAAAACATGATCCAAGCAGCATATATAAACTCTGCAACAATACCAAGTCAGGTCAGTTGCAACAATCAAGCAATCTTGCATCATCCTGCAAGCAATGAACATTCGAACCACCATCCAAACATTTTCAACCACCTTGCCAACCATCTTGCATACCATTGCGCCAACACTGACCAACCAACCACAAGCCAACAGACCAGCAATCCTGCACCACATCTTGGTAAGGCGTCATTTCTGACTCTGGCGCTGCTCCGGTGAGTCTTCATCTTCTTCTTCACCATTTCACTATGTAGGAGTTACCAGTATGTAGCATAAGGTTAGGGGAGTCAAAGCCCTAACCTCTAGGGATTTTACTCCGAGAGAGTGTGATTTAATTTCTTCTTGAATTGCTAGGGTTCTTCAGATAATTTACTTTGATTCGAGTTTTTTTTAGGAATTAGACATATACACTTTGATTTTTGGATTTAGGCTATGAAACTGGATTAGATGAGGTATTGATTATGGTCATTGAGTATCCTCCTCCGTCGGAGGCGACCTCCAGCGCGGCGGAGGGGGAGCGAAGGTTAGGAGAAAGGAGAACATGTTTTGAATTTACTTCTCAGTTTC
TARGET=571,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03950 Ps_contig_mira-and-tgicl-ass_franssen_31562
SEQUENCE=TATTGCTTCCTCCACCTTTTCCTTGATAAGATTTTGGTGCTTTCTTGTCTCATACTAGTATTTTCATTGCCAATAACTCAATACAATCATTTCATATATCTTTCTTATCATATTTCTCAAGTTTGAAGCTTGAGAGTTTTCTAGAACTAAGGGTTTATTTTTGTTATATACTTTGATGCCTTCAAATTTAGTAAGAAAAGGACATGTGTGCTGAGTTGGTTTTTGGTTTTGGTTTTGGTTTGGATNCCTTTCCACGATAGCAAAGTAGGGTAGTGCAAGTTGGATATCAAGATAAGCTAACTGTTTTGCTAGGTCTATTTTTAGCATTGATTTGAGATTCTTATTCTGTGCTGATATACATATCTTTTTACTTTTTGACAAATTTTCAGATCCAATTTCTAGGGTATCAATTATTATTCTTGAGATATTTTTATGTTTGAGAGAGAGAAACCATTTTCAACTTTTGTTTAGAGGTGAAGTTTAGAGTTATTGTAGTATATTAAGTTTAGAGGTTTGGTTAAGGCATGAAAGTTTGAAGCTTTAGATAGAACATGGGTAGTAATAGAACCATTGTTTGGTTTAGGAGGGATCTTAGAATTGAGGATAATCCAGCACTAGCTGCTGCTGCTAGGGATGGTTCTGTGTTTCCTGTGTTTATATGGTGTCCTAAAGAGGAAGGACAGTTTTATCCGGGCCGAGTTTCGAGGTGGTGGTTGAAGCAATCTCTTGCTCATTTGGATCAGTCATTGAAATCTCTTGGAGCTAAACTTGTGTTGATCAAAACTGATAGTACTCTTAATGCTCTTTTGGAGTGTGTAAATGCTATTCAAGCTACAAAAGTAGTGTTTAACCATCTCTATGATCCTGTTTCACTTGTTCGCGACCACAACATCAAAGAGAAATTAGTTGAACTTGGCATATCTGTGAAAAGTTACAATGGGGATCTGTTGTATGAGCCTTGGGAGTTATATGATGAGAAGGGACATGCTTTTACAACCTTTGATCCTTTTTGGGAGAGATGTTTGCACAAGCAAATGGAACCTGTTTCACTTATTCCTCCGTGGCAGTTAATTCCAGCTAAAGGAAAAGTAGAGAGATGTTCAATAGAGGACCTAGGTCTTGAAAACGAATTAGAAAAACCAAGCAACGCGTTACTAGGACGAGCATGGTCTCCAGGTTGGGGCAATGCTAACAAGGCTTTAACAGAGTTTATGGACAAGCAACTACTTAACTATTCCAAGAATCGGCAAAAGGTTGGTGGAGACTCCACCTCACTCTTGTCCCCGTATCTTCACTTTGGAGAGCTGAGCGTGAGGAAAGTTTTTCAAATGGCGCGTGTGAAGCAGATATCGTGGGGAAATGAAGGCAACAGTGTTGGCAAAGAAAGCGTAACTCTTTTCCTCAGGGCAATTGGTCTTAGAGAGTATTCGCGCTATCTTTGTTTCAATTTTCCGTTCACTCATGAGAGAGCACTTCTTGGTCACTTGAGTTTTTTCCCTTGGAATGCCGATCCTTCTAACTTTAAGACTTGGAGACAAGGTAGGACCGGCTATCCTTTAGTTGATGCAGGAATGAGAGAACTTTGGGCTACAGGATGGATGCATAACCGAATAAGAGTAATAGTTTCCAGTTTTGCTGTGAAAATGTTGCTTCTGCCTTGGAAATGGGGAATGAAGTATTTTTGGGACACACTTTTGGATGCAGACCTTGAATGTGATATATTAGGGTGGCAGTATATCTCAGGATGCTTACCAGATGGTCATGAGCTCGAGCGCTTGGATGATCCCGAGATTTTAGGAGCAAAATTTGATCCAGAAGGAGAATATGTAAGGCAATGGCTACCCGAGTTAGCAAGAATGCCGACCGAGTGGATCCATCATCCTTGGAATGCACCACTCAGTGTGCTTAGAGCATCAGGAGTTGAGTTAGGTCAAAACTATCCAAATCCAATAATTGATATAGATTTAGCCAGAGAAAAACTCACTCAAGCCATATTCAAGATGTGGGAAATTCAAGCAGCATCGAAAGCTTCTGGCTCCGAAGCAAGGGATGAAGTTGTCGTCGACAACGAAAATCAAGACATTCCAAAAGTTATCCTAAAGGACAAGGGTCCATGTGTTACTATCTCAGCTAATGATCAGAAAGTGCCAGCACTTCCGGATCCTAAGAATGAACTACCGGTTAGAAAGAGAAAGAAAGGTATGGAAGAGAAGGGAAAGGAGCAGGAAAGTTCGGTAAATAATGAGAAAGATAGTAAGGTTTCAAGCCCTGACCAAGAGACTTGCTCTACAGCTGATTCTTCTGCATGCAAGAAGCAGTGTAGTACAAGCACGTATACGTTTTCTGTTCCACAACAGTGTTCTTCATCTTCGAATCTAAAATGGTCATGGCAAGAACAGATTGACATGGAGCAAAGTTCAGGGAAAGATGGGCCAACATGAGGTTAGTGATGTTAATCCAGCATAAATGAAGTGTAATCTGTTTTCTGTGGCTATATTCTGAATAAAATGGTTCTGAAATCGAATTGAAAGAATAGCCAACAATGTATATTATCTACTTGTTCGAATCAGAAGTCCTTAAATATTCTGACAATCTTGTATACAAACTTCATTAACTAATCTACTACACAATAAATTATTCTCACTAGGCATAATTCATGGTGCATTTACCTTGTTTTTTCTTCAAAACTTGAGGCCTATAAAACATAATACTGACATGTAGACACTCTTCCGAATATGATGTGTCTGGATTACTTGATATTGAATATTCATATGATTCATTCACTATATAAC
TARGET=439,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0219
PRIMER_LEFT_PENALTY=0.021195
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=TTCCACGATAGCAAAGTAGGGT
PRIMER_RIGHT_SEQUENCE=TTCATGCCTTAACCAAACCTCT
PRIMER_LEFT=249,22
PRIMER_RIGHT=528,22
PRIMER_LEFT_TM=60.021
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.3652
PRIMER_LEFT_1_PENALTY=0.021195
PRIMER_RIGHT_1_PENALTY=0.343977
PRIMER_LEFT_1_SEQUENCE=TTCCACGATAGCAAAGTAGGGT
PRIMER_RIGHT_1_SEQUENCE=TTTCATGCCTTAACCAAACCTC
PRIMER_LEFT_1=249,22
PRIMER_RIGHT_1=529,22
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=60.344
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.4817
PRIMER_LEFT_2_PENALTY=0.021195
PRIMER_RIGHT_2_PENALTY=0.460533
PRIMER_LEFT_2_SEQUENCE=TTCCACGATAGCAAAGTAGGGT
PRIMER_RIGHT_2_SEQUENCE=CTTTCATGCCTTAACCAAACCT
PRIMER_LEFT_2=249,22
PRIMER_RIGHT_2=530,22
PRIMER_LEFT_2_TM=60.021
PRIMER_RIGHT_2_TM=59.539
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.4821
PRIMER_LEFT_3_PENALTY=0.481326
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=TTTCCACGATAGCAAAGTAGGG
PRIMER_RIGHT_3_SEQUENCE=TTCATGCCTTAACCAAACCTCT
PRIMER_LEFT_3=248,22
PRIMER_RIGHT_3=528,22
PRIMER_LEFT_3_TM=60.481
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.5853
PRIMER_LEFT_4_PENALTY=0.584545
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=NCCTTTCCACGATAGCAAAGTA
PRIMER_RIGHT_4_SEQUENCE=TTCATGCCTTAACCAAACCTCT
PRIMER_LEFT_4=245,22
PRIMER_RIGHT_4=528,22
PRIMER_LEFT_4_TM=59.415
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_03951 Ps_contig_mira-and-tgicl-ass_franssen_31562
SEQUENCE=TATTGCTTCCTCCACCTTTTCCTTGATAAGATTTTGGTGCTTTCTTGTCTCATACTAGTATTTTCATTGCCAATAACTCAATACAATCATTTCATATATCTTTCTTATCATATTTCTCAAGTTTGAAGCTTGAGAGTTTTCTAGAACTAAGGGTTTATTTTTGTTATATACTTTGATGCCTTCAAATTTAGTAAGAAAAGGACATGTGTGCTGAGTTGGTTTTTGGTTTTGGTTTTGGTTTGGATNCCTTTCCACGATAGCAAAGTAGGGTAGTGCAAGTTGGATATCAAGATAAGCTAACTGTTTTGCTAGGTCTATTTTTAGCATTGATTTGAGATTCTTATTCTGTGCTGATATACATATCTTTTTACTTTTTGACAAATTTTCAGATCCAATTTCTAGGGTATCAATTATTATTCTTGAGATATTTTTATGTTTGAGAGAGAGAAACCATTTTCAACTTTTGTTTAGAGGTGAAGTTTAGAGTTATTGTAGTATATTAAGTTTAGAGGTTTGGTTAAGGCATGAAAGTTTGAAGCTTTAGATAGAACATGGGTAGTAATAGAACCATTGTTTGGTTTAGGAGGGATCTTAGAATTGAGGATAATCCAGCACTAGCTGCTGCTGCTAGGGATGGTTCTGTGTTTCCTGTGTTTATATGGTGTCCTAAAGAGGAAGGACAGTTTTATCCGGGCCGAGTTTCGAGGTGGTGGTTGAAGCAATCTCTTGCTCATTTGGATCAGTCATTGAAATCTCTTGGAGCTAAACTTGTGTTGATCAAAACTGATAGTACTCTTAATGCTCTTTTGGAGTGTGTAAATGCTATTCAAGCTACAAAAGTAGTGTTTAACCATCTCTATGATCCTGTTTCACTTGTTCGCGACCACAACATCAAAGAGAAATTAGTTGAACTTGGCATATCTGTGAAAAGTTACAATGGGGATCTGTTGTATGAGCCTTGGGAGTTATATGATGAGAAGGGACATGCTTTTACAACCTTTGATCCTTTTTGGGAGAGATGTTTGCACAAGCAAATGGAACCTGTTTCACTTATTCCTCCGTGGCAGTTAATTCCAGCTAAAGGAAAAGTAGAGAGATGTTCAATAGAGGACCTAGGTCTTGAAAACGAATTAGAAAAACCAAGCAACGCGTTACTAGGACGAGCATGGTCTCCAGGTTGGGGCAATGCTAACAAGGCTTTAACAGAGTTTATGGACAAGCAACTACTTAACTATTCCAAGAATCGGCAAAAGGTTGGTGGAGACTCCACCTCACTCTTGTCCCCGTATCTTCACTTTGGAGAGCTGAGCGTGAGGAAAGTTTTTCAAATGGCGCGTGTGAAGCAGATATCGTGGGGAAATGAAGGCAACAGTGTTGGCAAAGAAAGCGTAACTCTTTTCCTCAGGGCAATTGGTCTTAGAGAGTATTCGCGCTATCTTTGTTTCAATTTTCCGTTCACTCATGAGAGAGCACTTCTTGGTCACTTGAGTTTTTTCCCTTGGAATGCCGATCCTTCTAACTTTAAGACTTGGAGACAAGGTAGGACCGGCTATCCTTTAGTTGATGCAGGAATGAGAGAACTTTGGGCTACAGGATGGATGCATAACCGAATAAGAGTAATAGTTTCCAGTTTTGCTGTGAAAATGTTGCTTCTGCCTTGGAAATGGGGAATGAAGTATTTTTGGGACACACTTTTGGATGCAGACCTTGAATGTGATATATTAGGGTGGCAGTATATCTCAGGATGCTTACCAGATGGTCATGAGCTCGAGCGCTTGGATGATCCCGAGATTTTAGGAGCAAAATTTGATCCAGAAGGAGAATATGTAAGGCAATGGCTACCCGAGTTAGCAAGAATGCCGACCGAGTGGATCCATCATCCTTGGAATGCACCACTCAGTGTGCTTAGAGCATCAGGAGTTGAGTTAGGTCAAAACTATCCAAATCCAATAATTGATATAGATTTAGCCAGAGAAAAACTCACTCAAGCCATATTCAAGATGTGGGAAATTCAAGCAGCATCGAAAGCTTCTGGCTCCGAAGCAAGGGATGAAGTTGTCGTCGACAACGAAAATCAAGACATTCCAAAAGTTATCCTAAAGGACAAGGGTCCATGTGTTACTATCTCAGCTAATGATCAGAAAGTGCCAGCACTTCCGGATCCTAAGAATGAACTACCGGTTAGAAAGAGAAAGAAAGGTATGGAAGAGAAGGGAAAGGAGCAGGAAAGTTCGGTAAATAATGAGAAAGATAGTAAGGTTTCAAGCCCTGACCAAGAGACTTGCTCTACAGCTGATTCTTCTGCATGCAAGAAGCAGTGTAGTACAAGCACGTATACGTTTTCTGTTCCACAACAGTGTTCTTCATCTTCGAATCTAAAATGGTCATGGCAAGAACAGATTGACATGGAGCAAAGTTCAGGGAAAGATGGGCCAACATGAGGTTAGTGATGTTAATCCAGCATAAATGAAGTGTAATCTGTTTTCTGTGGCTATATTCTGAATAAAATGGTTCTGAAATCGAATTGAAAGAATAGCCAACAATGTATATTATCTACTTGTTCGAATCAGAAGTCCTTAAATATTCTGACAATCTTGTATACAAACTTCATTAACTAATCTACTACACAATAAATTATTCTCACTAGGCATAATTCATGGTGCATTTACCTTGTTTTTTCTTCAAAACTTGAGGCCTATAAAACATAATACTGACATGTAGACACTCTTCCGAATATGATGTGTCTGGATTACTTGATATTGAATATTCATATGATTCATTCACTATATAAC
TARGET=618,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5415
PRIMER_LEFT_PENALTY=0.460533
PRIMER_RIGHT_PENALTY=0.080962
PRIMER_LEFT_SEQUENCE=AGGTTTGGTTAAGGCATGAAAG
PRIMER_RIGHT_SEQUENCE=TCAATGACTGATCCAAATGAGC
PRIMER_LEFT=509,22
PRIMER_RIGHT=749,22
PRIMER_LEFT_TM=59.539
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=0.6655
PRIMER_LEFT_1_PENALTY=0.584504
PRIMER_RIGHT_1_PENALTY=0.080962
PRIMER_LEFT_1_SEQUENCE=GGTTTGGTTAAGGCATGAAAGT
PRIMER_RIGHT_1_SEQUENCE=TCAATGACTGATCCAAATGAGC
PRIMER_LEFT_1=510,22
PRIMER_RIGHT_1=749,22
PRIMER_LEFT_1_TM=59.415
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=0.7282
PRIMER_LEFT_2_PENALTY=0.343977
PRIMER_RIGHT_2_PENALTY=0.384229
PRIMER_LEFT_2_SEQUENCE=GAGGTTTGGTTAAGGCATGAAA
PRIMER_RIGHT_2_SEQUENCE=GTCCTTCCTCTTTAGGACACCA
PRIMER_LEFT_2=508,22
PRIMER_RIGHT_2=680,22
PRIMER_LEFT_2_TM=60.344
PRIMER_RIGHT_2_TM=59.616
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.7282
PRIMER_LEFT_3_PENALTY=0.343977
PRIMER_RIGHT_3_PENALTY=0.384229
PRIMER_LEFT_3_SEQUENCE=GAGGTTTGGTTAAGGCATGAAA
PRIMER_RIGHT_3_SEQUENCE=TGTCCTTCCTCTTTAGGACACC
PRIMER_LEFT_3=508,22
PRIMER_RIGHT_3=681,22
PRIMER_LEFT_3_TM=60.344
PRIMER_RIGHT_3_TM=59.616
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.7989
PRIMER_LEFT_4_PENALTY=0.717980
PRIMER_RIGHT_4_PENALTY=0.080962
PRIMER_LEFT_4_SEQUENCE=AACCATTGTTTGGTTTAGGAGG
PRIMER_RIGHT_4_SEQUENCE=TCAATGACTGATCCAAATGAGC
PRIMER_LEFT_4=565,22
PRIMER_RIGHT_4=749,22
PRIMER_LEFT_4_TM=59.282
PRIMER_RIGHT_4_TM=60.081
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_03952 Ps_contig_mira-and-tgicl-ass_franssen_31563
SEQUENCE=TCGCGGGATCATTCTTTTTCTTGTTATGTAGGTTTCGTTTTCATATTATCATGAGCAATATCCCAAAATCTCTCACCGACTCTTCCTTAACCCTCTTCAATCTCACCATTTCTTCTTCCGATCCTTGGCCTTTCTCTTTTGTTTTCTTATAACACAGAAAATCAGTTCTTAACAACCTTGAATACGCTTGTTATTTTGTCCTTTTTAATTTCTTCTTAATTATGGGGTTTAGTGGTAATCACCCTCATCATCATAATTTGAGTTTTCATTTGCATATTCCTCATCTTCACTTTCATCAAAACCATGAAAAGAATAAGGACATTCCAAAAGGGTGTTTGGCAGTGATGGTGGGACAAGGAGAGGAGCAACAAAGGTTTGTGATTCCAGTGATTTACATCAACCATCCATTGTTTATGCAGTTGCTGAAAGAAGCTGAAGAAGAATATGGATTTGCTCAGAAAGGACCCATCACCATTCCTTGCCACATAGAACAGTTTCGCACCGTCCAAGGAATGATTGATATGGAAAAATCTCATCACCATCATCACCATGCTTGGTGTTTCAAAGTTTGATTCAATATTATTATTATTATCATTATTACTATCTATTTACACTTTTATGTATTTTTTGTATGTCAATATTCTCCAATGATGGATCTTAATTAGTAGAATTATAATTTGGAGTATATTGTTTGATATGAATGTAAATTGGAAAGTATTGAAAGAGAATCATATTGTTGTATGAGTATTAATTNCCAANNNNAAAAG
TARGET=578,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03953 Ps_contig_mira-and-tgicl-ass_franssen_31564
SEQUENCE=TTGCGTGTCTCAGAAGCAGTGGTATTTCAAYNCRGRGTAGTCGGGGACATACCAAAACAACAAACCAACCTCAAAACTCAAAATCAATGGCACCACCAAGCTTCTATTCACACCTCCTCCTCATAATTTGCGCCACCGTCTTGTTAACACTAACGATTTCACCACCGACAGTAGATGCCGGAGGATTCAACTTCGGAATGGAATGGATTCACCAAACCAAAACAACCTGCGAAGGTTCCATCGCCGATTGCATGTTGCAACAAGGTGAAGAAGAGTTTCAGTTTGATAACGAGATCAACAGGCGTATTTTAGCAACAACAAAGTACATCAGCTATGGTGCGCTTCAGAGGAACACTGTGCCATGCTCTCGCCGTGGCGCTTCGTACTACAATTGCCGTCCCGGTGCTCAGGCCAACCCTTACAGCCGTGGATGCAGCGCTATCACGAGGTGCCGGAGTTAATTAATTATTATTTTTCTTCAATTTTTGAAAAAAATAATAATTATTAATTAATTCAGTTTGGTTGTGAATTTTTATATACTTTAATATTGGACGTTTAATCCATTTTATTTGTGGGTCACTGTTGTGAGTTGTGACTCTTTTATGAAAATACTCTGTTATTTACCGTTGTTGATTATTGTTAATTATGTTATTATCAATTAATTATTTAATTGTTGTTATTATTAAAATTTAATTTTTTATGGTAAAAATATAAATATAAATATAAATATAAGAGAAATTAATAAATATTTATTTTTTTAATATTCTTATTATTACGATATTGCGCGTTGAATACAGCTATGTATGCCAGCGTGTTGGTGAATGTGTGTGTGTGACTGTAATGTATTGTCGTATTCACGATGTATGAATGATAATTGAATTACATTTATAGAAAA
TARGET=823,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03954 Ps_contig_mira-and-tgicl-ass_franssen_31566
SEQUENCE=GGTTTAATTATTTACTCTTAATTTTGTTGATTTCAACTATAACAAGCTGATTGATAATGATTGCACATGTTGGTAATAGAAGACAAGACAATATAGAAGCAAAGTTCATCTAACAATTACTCTTCTACTGAGAACTCAAGGTACACATTCTTGATAATTGAAGCAAAAACAAACTTTGTAAATGATATATCTGAAGATTGAAGGTAGAAATGAAGTTACAATTTATAAACCGCAATAAAAGGCATAATAACGCTTCCTATCCAAAGTTGACCATCTTTCTCCTCAACTTCACTGGCAGCTCTAACAACTTTTCCCTCAGTGTCCTCCAATATCTGCAGCAATTTACCTTCAGGACTATACTTCACAATCACACCATGTTGGTTTCCTCCAATATGAATCAGATATTGAAACTTCAATGGTATAGGAAGCTTGAGTATAACTTTACTTATCCATGGATATAAACCAGTAATGTATGAATACATAGTTCTTCTACAGTGAATTGCCACCCAAAAATCACCATCTTCATTCACCCTCACATTGTCGGGAGGTCCAGGTACGATTGCGATGATCTCTGAAGTTCCAGCCTTTTCTCCTTTTAGCCAATATTTACGTAACCTGCCAATATCCGCTTCACAAAAAATAAAGAAGGAACCATCTTTACTTAAGGAAATGCCATTTGGGAATTGAATGTTTCTCACAAGAACAGTGGTTTCCTTTGTTGTAGGGTTGTATTTCAAAACCTTGCCACTATCATCCCCGGAAGCTGCCAGCTGAAAAAAATTTCTCCTCTGATAAATTTCACTGCTATGGGTAAAATAAACATTTCCTTCGGCATCTACATCAACATCATTAGTAAATCTCAGAGGCACCCCTTCAACTTCAGTTGTAAGCGACGTTGCTAAGCCGCCCTCGGGCCCCACCTTCATGAGTCCAAGATATGCATCTGCAATATACAATTCACCTGTTTTCTTGTGAAATCTGAGTCCTAAAGGCCTTCCACAGATATGTTCAGTTTTCACGTAACTAAATGGCGTTGCAGCTTCAACAGGAAGAGGTTTACAGAATGATCTATTGGGCGAAGTGTAAGCGAAATCAACCCAATTATGCTCATCATTGAGGAACAAGATTCTACCATCAGCAACAGCGGTGTAAGGTCCACGACCAAGATTGTCGAAAACAATGCTCTCGGGTCCTTGTATTTGGTTCAAAAATCTAATATGAGAGTTTTGCAGCAAGTTGTGTTGGTCTCTGTCTTTAGGCAGTTCAGACCATGCTGGCGTGTGAATCTTTTTGACCTCAAAATTAGGGAAATTTGCAATGGGACTGTAGTTGAATGGGTCTAAACCGCAGTATAGAGCAAACAGGAGAAAGATAGCGGCGAAGATTTGAGTAAAAGTCATGGTATTGGAATGAGAGAGAAATGAATGATTGAAGGTTGAAGATATTAGTTGTTGAGTGAGTGAGTGAGTCAGTGTAGTTATTAGTGAAACTTTGAAGTGGGTTGGTAGCAGCAAACCCCAATTAGACTTGAAATGAGGTTTTATTATATAGAA
TARGET=1453,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0423
PRIMER_LEFT_PENALTY=0.041553
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=AGCAAACAGGAGAAAGATAGCG
PRIMER_RIGHT_SEQUENCE=TCAAGTCTAATTGGGGTTTGCT
PRIMER_LEFT=1355,22
PRIMER_RIGHT=1530,22
PRIMER_LEFT_TM=60.042
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.0871
PRIMER_LEFT_1_PENALTY=0.086339
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=TGTCTTTAGGCAGTTCAGACCA
PRIMER_RIGHT_1_SEQUENCE=TCAAGTCTAATTGGGGTTTGCT
PRIMER_LEFT_1=1249,22
PRIMER_RIGHT_1=1530,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=0.1103
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=AATGGGACTGTAGTTGAATGGG
PRIMER_RIGHT_2_SEQUENCE=TCAAGTCTAATTGGGGTTTGCT
PRIMER_LEFT_2=1316,22
PRIMER_RIGHT_2=1530,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=0.1849
PRIMER_LEFT_3_PENALTY=0.184188
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=GCAAGTTGTGTTGGTCTCTGTC
PRIMER_RIGHT_3_SEQUENCE=TCAAGTCTAATTGGGGTTTGCT
PRIMER_LEFT_3=1231,22
PRIMER_RIGHT_3=1530,22
PRIMER_LEFT_3_TM=59.816
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.2076
PRIMER_LEFT_4_PENALTY=0.206859
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=CGGCGAAGATTTGAGTAAAAGT
PRIMER_RIGHT_4_SEQUENCE=TCAAGTCTAATTGGGGTTTGCT
PRIMER_LEFT_4=1375,22
PRIMER_RIGHT_4=1530,22
PRIMER_LEFT_4_TM=59.793
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=156
=
PRIMER_SEQUENCE_ID=SSR_03955 Ps_contig_mira-and-tgicl-ass_franssen_31573
SEQUENCE=AGTACGTCGGGGRACGGATAAGAAAATCTGAATAAATAAACGGAACCCNCACTTTCTCNNGGGATTCGCTCCATCATCATCATCCACACAGTAATCATCAAATTTTCTTCTCACTACTTCACTTCACTAATCAACCTTCAACTTAAACCCTACCACACTATAATCCACTACTCAATTTTTACTGGAGCGAGATGAGTACTTCTTCTATCGACGATTTTCCCGCGGTTCAGGAGGTTATGCTGGAGTTTCGTGCTGGTAAAATGCTTCTAGAAGGAAAACGGGTGGTTCCTGATGCTCGAAAAGGGCTTGTTCGGATTGCTAGGGGTGAGGAGGGATTAGTTCATTTTCAGTGGCTTGATCGCACGCTAAATGTTCTCGAAGATGATCAGATCATATTTCCAAACGAGGCTACTTTTGAGCAGGTTAATCAAGCATCTGGAAGGATTTATATATTGAAGTTTAATAGTGATGACAGAAAGTTCTTCTTCTGGATGCAGGAATCTAATGCTGATAATGACTCGCAACTTTGTAGCTCTGTGAATGATTATATTAACAGACCATTGGAGCTCCTTGGCGAAGAGGAACCTGATGGATCTCTTCCACTTCAAGTATCTGAAGATATGGCTGAGGATGATATCTCATCTAGAGCTGCAAACTTATTTGTCCCAAACCTCGGTGTGGATGCAACTAGTGATGTATCGTCTTCTGGTCCAGTAAAATTGGAAGATCTCCAAAGAATATTGAGTAACATTGGTCCAGCTGCAGATAATATTATTGATCCTGATGGAGGCTTTGAACTCGGTGACCTATTGAAGCCTGATCTGATATTGCCATTGATGGAGACATTATCATTAGAGCAGCGTCTTGCTCCTTATTTACCGGAGGGTACTTGGTCTCCAAAAGAGATATTGGAGTTGTTGCAGAGCCCACCTTTTCGTCAGCAAGTAGATTCATTTACTTATGTACTTCGAACAGGACAGATAGATTTGTCTCAGTTTGGAATTGATCCAAGTAAATACAAGTTCACAGTTTTGTCTTTCCTTGAGGCTCTTGAGGATTCTGTTTCCAAGTCTGAGGAAACCAGGCAAGATGATCAGGAATTAGTATCTCAATCTTGTACTCCAATGGATGAATCAAAGTAGCTTGGTCTTTTTGTCTTTTTTCTATAGATACAAGGGTAATTTTTGTTTTTTTTTTTGTGGCAAAACATNTATATGCTT
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03956 Ps_contig_mira-and-tgicl-ass_franssen_31580
SEQUENCE=GTATCAACGCAGAGTACTCGGGGAACCAACTAACAATACAATTAACAATTTAACACCAACAACACTCAAGCTCAATCACTTCAAATATTTCGAGATCTGAAATCAAGAGAGAGAGAGAAACGGAAATGAGGGAGCTGTCGTCGGTGGGAACTGAAAGCAGTAGCGTCGCTTCTCCGGCGCCGCCGCCTCGAGCACTTCTCGAGAGACTCAAAGACTACGGTCAGGAAGACGTTTTGGCTCTCTGGGATGAGATTTCTCATGAGGAACGTGATTTTCTCGTTAAGGAGATTGAGAGTTTAGATCTTTCGAGAATTGACCGGATTATTCGGTGTTCGTTGCGATCTCAAGCTCTACCGGCAGCTGTAATTGAACCTGTGCCGGAGAGCAATGTTTCGAAGGTGGAGGAGCGAAGTCAAGAGCAACGAGACAAATGGTGGAAAATGGGGTTGAAGGCTATTTCAGACGGCAAGTTAGCTGTTTTGCTTTTATCCGGTGGCCAGGGGACTAGGCTTGGAAGTTCAGATCCAAAAGGATGTTTCAATATTGGACTTCCATCTGGAAAATCACTTTTTCAACTTCAAGCCGAGAAGATTTTGTGTGTACAGAGATTAGCTGCTCAAGCTACAAATGAGAGTTCTGCTTCTTCGGTGCAAATAAACTGGTACATAATGACTAGTCCATTCACTGATGAGGCAACGCGTAAATTCTTTGAAAGTCATAAATATTTTGGTCTTGAAGCAGAACAGGTTACCTTTTTCCAGCAAGGGACCATCCCGTGTGTTTCCAAAGATGGTAGAATTATTTTGGAGACTCCGAATAGGGTAGCAAAGGCTCCTGATGGGAACGGTGGAGTGTATTCAGCTCTGAAGTCTACCAAATTATTGGAGGACATGGCCTCAAAAGGAATTAAATATATTGATTGCTATGGAGTAGACAATGCACTGGTTCGAGTTGCTGATCCATCTTTTTTAGGTTACTTCATTGATAAAGGTGTCGCTGCTGCAGCTAAAGTCGTCCGCAAGGCATATCCCCAAGAAAAGGTTGGAGTGTTTGTACAGAGAGGTAAAGGTGGTCCTCTCACCGTAGTTGAATACAGCGAGTTGGACTCATCACTAGCTTCCGCGGTTAATCAAGCAACTGGCCGTCTTCGTTTTTGTTGGAGCAATGTTTGCTTACACATGTTTACTTTGGATTTTCTAAACCAAGTTGCAAATGGACTTGAAAAGGACAGCATTTACCACCTTGCCGAGAAGAAAATCCCTTCCACACATGGATATACTATGGGATTAAAACTTGAACAGTTTATATTTGATGCATTTCCATATGCTTCTACCACTGCACTTTTTGAGGTATTACGGGAGGAGGAGTTTGCGCCTGTAAAAAATGCAATTGGATCCAATTATGACACTCCAGATACTGCTAAAATGCTTGTTTTTCGACTACATACTCGTTGGGTAGTTGCTGCAGGTGGCTTCTTAACACATTCAGTGCCCTTATATGCAACAGGTGTTGAAGTGTCACCACTCTGTTCATATGCGGGTGAAAACCTGGAACCTATATGTCGAGGAAGAACATTTCACGCACCTTGTGAAATTACGTTCTAGTTTCACCCTGTTTGTGCTGCTTCGTTGCGACTTAATCTGCAATGAGGGAGTTCTCAGCCCCATACAGGTGTCTGAAGATAATTTTTTATCTATGCCACTCATCATTCACATGTATCAATATATCTAATTATCTGTATCTTCTCCGATTCGTTTGGCTTTTTTGGTTGTTTTTTTTGAACTTTTTTTCTATCACTGGTCTGTTTTATTGTTGCTTAAGTGATGTACACGTATCATATTCTTTATCGGCCATTTATTTACCCATGTAAAATCAAATATTTCCATTTTTTTCTCCTATTCCCCGTGGCTCATAATGAACTGTGGTACTGTCAATACTCAATATTGTTTTACTTGCTCCATATTCTTAGAAACATTGTATAATAAGAGAAAAAATTTAAAATTTG
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1133
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=TCGGGGAACCAACTAACAATAC
PRIMER_RIGHT_SEQUENCE=ACGAGAAAATCACGTTCCTCAT
PRIMER_LEFT=17,22
PRIMER_RIGHT=279,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.2242
PRIMER_LEFT_1_PENALTY=0.219884
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=CCAACAACACTCAAGCTCAATC
PRIMER_RIGHT_1_SEQUENCE=ACGAGAAAATCACGTTCCTCAT
PRIMER_LEFT_1=55,22
PRIMER_RIGHT_1=279,22
PRIMER_LEFT_1_TM=59.780
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=0.2338
PRIMER_LEFT_2_PENALTY=0.229480
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=CTCGGGGAACCAACTAACAATA
PRIMER_RIGHT_2_SEQUENCE=ACGAGAAAATCACGTTCCTCAT
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=279,22
PRIMER_LEFT_2_TM=60.229
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=264
PRIMER_PAIR_PENALTY_3=0.2791
PRIMER_LEFT_3_PENALTY=0.274768
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=GTACTCGGGGAACCAACTAACA
PRIMER_RIGHT_3_SEQUENCE=ACGAGAAAATCACGTTCCTCAT
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=279,22
PRIMER_LEFT_3_TM=60.275
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.2985
PRIMER_LEFT_4_PENALTY=0.108929
PRIMER_RIGHT_4_PENALTY=0.189529
PRIMER_LEFT_4_SEQUENCE=TCGGGGAACCAACTAACAATAC
PRIMER_RIGHT_4_SEQUENCE=CGTCTTCCTGACCGTAGTCTTT
PRIMER_LEFT_4=17,22
PRIMER_RIGHT_4=230,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=59.810
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_03957 Ps_contig_mira-and-tgicl-ass_franssen_31582
SEQUENCE=AAAAGAAGATTCATTTGATTGAGGCGTGAATTGTTTTCAGTAACAGTCGCCTACGTTTCTTTCCTTCCTTCCTCGTACACGCACTCTACACGTTTCTCTCTCTAATTTCCCAAATTACCATTTTTGAACCATTAAACTTATTACTCTACTTAATTTCAACCCTTTTTCATTCCATTCAATTTTCGCTTCGATCTAATCAATGGGTGCCGCAGATGAAGAATACGATTACTTGTTCAAGTTGGTTTTGATCGGTGATTCTGGTGTTGGTAAGTCCAATCTACTCTCTAGATTCACCAGAAATGAATTCAACTTGGAATCCAAATCTACTATTGGTGTTGAATTTGCGACCAGAAGTGTTCGGATTCTTGATAAACTTGTTAAGGCTCAGATTTGGGATACTGCTGGACAAGAAAGATATCGTGCAATCACAAGTGCTTACTACCGTGGAGCCGTTGGTGCATTGCTTGTGTACGATTCAACAAGGCACGTGACATTTGAAAACGTGGAGAGATGGTTGAAGGAGCTTCGAGATCACACTGATGCCTACGTTGTAATCATGCTTGTAGGAAACAAAACAGATCTTCGACACCTGTGTGCCGTGTCCACAGAAGAAGCCACCGCATTTGCTGAGAAAGAAAACATATATTTCATGGAAACTTCTGCACTCGAGTCGATGAATGTGGACAGCGCGTTTGTGGAAGTGCTCACTCAGATATACAATGTTGTGAGTAAGAGGGCTCTTGAGAAAGAGAACGGGTCTGCATCAGTGCCTAAGGGAGAGACTATCAATATTGGTAGAGATGATGTCTCAGAGGTTAAGAAGAGTACATGCTGCAGTACAGCGTAGTTTGAATTGAAAGAAGATCTTAGTTACAGAGTAAAATTATCATTATTTTGGATCCATATTAAATGTTTTGGTATTTGATTGATACCTTTTTATATTTTGCCATTGAACATTTTTCTTATTTGTTGTGAGAAAGTCATTGCTTTATGATGCGTTTTTGTTACTGAGATACATGATTTGCTAAGAGCCAATTGTTGTTGTTCTTACATTCTCTC
TARGET=61,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7643
PRIMER_LEFT_PENALTY=0.535872
PRIMER_RIGHT_PENALTY=0.228464
PRIMER_LEFT_SEQUENCE=TTTTCAGTAACAGTCGCCTACG
PRIMER_RIGHT_SEQUENCE=AGAATCACCGATCAAAACCAAC
PRIMER_LEFT=33,22
PRIMER_RIGHT=258,22
PRIMER_LEFT_TM=59.464
PRIMER_RIGHT_TM=60.228
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=226
PRIMER_PAIR_PENALTY_1=0.7643
PRIMER_LEFT_1_PENALTY=0.535872
PRIMER_RIGHT_1_PENALTY=0.228464
PRIMER_LEFT_1_SEQUENCE=TTTTCAGTAACAGTCGCCTACG
PRIMER_RIGHT_1_SEQUENCE=GAATCACCGATCAAAACCAACT
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=257,22
PRIMER_LEFT_1_TM=59.464
PRIMER_RIGHT_1_TM=60.228
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=0.7979
PRIMER_LEFT_2_PENALTY=0.535872
PRIMER_RIGHT_2_PENALTY=0.261998
PRIMER_LEFT_2_SEQUENCE=TTTTCAGTAACAGTCGCCTACG
PRIMER_RIGHT_2_SEQUENCE=ACCGATCAAAACCAACTTGAAC
PRIMER_LEFT_2=33,22
PRIMER_RIGHT_2=252,22
PRIMER_LEFT_2_TM=59.464
PRIMER_RIGHT_2_TM=60.262
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.9576
PRIMER_LEFT_3_PENALTY=0.729140
PRIMER_RIGHT_3_PENALTY=0.228464
PRIMER_LEFT_3_SEQUENCE=GAGGCGTGAATTGTTTTCAGTA
PRIMER_RIGHT_3_SEQUENCE=AGAATCACCGATCAAAACCAAC
PRIMER_LEFT_3=20,22
PRIMER_RIGHT_3=258,22
PRIMER_LEFT_3_TM=59.271
PRIMER_RIGHT_3_TM=60.228
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=0.9576
PRIMER_LEFT_4_PENALTY=0.729140
PRIMER_RIGHT_4_PENALTY=0.228464
PRIMER_LEFT_4_SEQUENCE=GAGGCGTGAATTGTTTTCAGTA
PRIMER_RIGHT_4_SEQUENCE=GAATCACCGATCAAAACCAACT
PRIMER_LEFT_4=20,22
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=59.271
PRIMER_RIGHT_4_TM=60.228
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_03958 Ps_contig_mira-and-tgicl-ass_franssen_31583
SEQUENCE=CATCATTTAGAGRACCTCTAGAGGCATAAATCAAAATCAGTTCTGTCTTATTCTTGCCTCTTGGGACCTTGCTGCTGTGTACTTCACCTCTTGTGGGGATGTCCTTGTTCTTCTCTCCATCAAAAATATACCATAAAACTACACCCTCCCTTCATCTTATAGAGGTAACAAAACTTCTCAAATTATAGTATCAAGATTCAAGTTTTTTCTTTGAAGCTTCTTGTTCCAAGAAAGGAAGAGAAAGAATGGATGATAAGTATAGGCTAGTTAGGAAGCATAGTGAGCTATGGAGAACATTGAGAGATGGAGATTTTGAAGAAGAAGAAATTTGGGATGTTGTGAAAGAAAGAGAAGATTACACATGCGAAGAAGTTTTTCACCACAAGGCAAAGAACAAAGAACTATCATCTCTTCTGATTCCGATTGGTTCAAGAACGATTCCGATTCCGAGGACTAGTAGTGAGAGTAGTAGTGTGAATTCATCAGCACCGGTTAATATTCCTAATTGGTCAAAGATTTATGGTCATGATGATGATAAGGTAAACAAGAGTGTTAAGAATGTTTCAAGGTATGGTAATGATAATTATGGTTACTATGATGATCATGAAGGTGGTGATGATCATGAAGTTTTGAACCATGGCGAAAGAGGTAGCGATGATGATGATGATGGTGAAGATGAAGATGAAGATGATGATGGTGAGTATGGTACTAGGCTGCCACCTCATGAGATAATTGCAAGAAGGCTTGCAAGGAGTCAGATTTCTTCATTTTCTGTTTTTGAAGGTGTTGGTAGGACACTTAAGGGTAGGGATCTTAGCAAAGTGAGGAACTCTGTCCTCATAAAGACTGGTTTTCTTGAATCATTATGATTAAGGGTTTTTCAATTGATCAAATTTGTGAGATTAAGTACATGGTTTTAAATTACGGTTGCGAACTTTTGTATTGCATTAAATTTTGAGAATTTGAAATTGCAGTTGTAATATAGTTGCAAGGACTTCTCAATATTTTCGGATGCAGTACTATAATTTAAAACCATAGTTAGTTATAGTTAGAGTCGTCTTTAACGACACGTCTTTAAGATCGTGCAAATGATTATTACACTGAAATTTAAAATTTGTCATTGTTTAAGTCACGAAGTAAATAGGATCTCAATAAATTATTTATCAAGTATTTAGTCCTAGAACTAAAGAGTGGTCYATTATTAGTTTAGGTTATTGTATTATT
TARGET=315,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03959 Ps_contig_mira-and-tgicl-ass_franssen_31583
SEQUENCE=CATCATTTAGAGRACCTCTAGAGGCATAAATCAAAATCAGTTCTGTCTTATTCTTGCCTCTTGGGACCTTGCTGCTGTGTACTTCACCTCTTGTGGGGATGTCCTTGTTCTTCTCTCCATCAAAAATATACCATAAAACTACACCCTCCCTTCATCTTATAGAGGTAACAAAACTTCTCAAATTATAGTATCAAGATTCAAGTTTTTTCTTTGAAGCTTCTTGTTCCAAGAAAGGAAGAGAAAGAATGGATGATAAGTATAGGCTAGTTAGGAAGCATAGTGAGCTATGGAGAACATTGAGAGATGGAGATTTTGAAGAAGAAGAAATTTGGGATGTTGTGAAAGAAAGAGAAGATTACACATGCGAAGAAGTTTTTCACCACAAGGCAAAGAACAAAGAACTATCATCTCTTCTGATTCCGATTGGTTCAAGAACGATTCCGATTCCGAGGACTAGTAGTGAGAGTAGTAGTGTGAATTCATCAGCACCGGTTAATATTCCTAATTGGTCAAAGATTTATGGTCATGATGATGATAAGGTAAACAAGAGTGTTAAGAATGTTTCAAGGTATGGTAATGATAATTATGGTTACTATGATGATCATGAAGGTGGTGATGATCATGAAGTTTTGAACCATGGCGAAAGAGGTAGCGATGATGATGATGATGGTGAAGATGAAGATGAAGATGATGATGGTGAGTATGGTACTAGGCTGCCACCTCATGAGATAATTGCAAGAAGGCTTGCAAGGAGTCAGATTTCTTCATTTTCTGTTTTTGAAGGTGTTGGTAGGACACTTAAGGGTAGGGATCTTAGCAAAGTGAGGAACTCTGTCCTCATAAAGACTGGTTTTCTTGAATCATTATGATTAAGGGTTTTTCAATTGATCAAATTTGTGAGATTAAGTACATGGTTTTAAATTACGGTTGCGAACTTTTGTATTGCATTAAATTTTGAGAATTTGAAATTGCAGTTGTAATATAGTTGCAAGGACTTCTCAATATTTTCGGATGCAGTACTATAATTTAAAACCATAGTTAGTTATAGTTAGAGTCGTCTTTAACGACACGTCTTTAAGATCGTGCAAATGATTATTACACTGAAATTTAAAATTTGTCATTGTTTAAGTCACGAAGTAAATAGGATCTCAATAAATTATTTATCAAGTATTTAGTCCTAGAACTAAAGAGTGGTCYATTATTAGTTTAGGTTATTGTATTATT
TARGET=654,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03960 Ps_contig_mira-and-tgicl-ass_franssen_31588
SEQUENCE=GGGACAAGCACATTACACAACACAACACAACACACAAACATAAACAGAGGTAGCGTTTGTATTCAGTTATATTATTGTTCTTGTCTGAGAAGAACAAAACCTGAAAATGGCCATGGATTGCGGCATCTATGGCGTCATCAAGCTCCGCACGTTCTTCACTTTCCTCATCCTCTTTCTCTCTACCTCCACCTTTTCCCTCTCATCTTCTTCTTCTTCAAATGTTCAAATAAACTCAAACTCAATCTTGGTGGCTCTTCTCGACTCACATTACACAGAACTCGCTGAACTCGTTGAAAAAGCCATGCTTCTTCAAACACTAGAAGACACTGTCGGCAACAGCAACATCACCATTTTCGCACCCAAAAACGAAGCTCTTGAACGAGATCTCGACCCTGACTTCAAAACGTTCCTCCTCGAACCACGAAATCTCAAGTCTCTTCAAACTCTCCTCATGTCACACATCATTCCAACGCGAATCAACGGATCCGAACCCGGGTCGACCCGACACAAAACGCTTTGCCTTCAACACCACCTCACTATCCAACCAAACGAAACTTCTCAACAATGGACAGTTAACGGCGCTAAGATTCTCCATCTAAACGCCGTCACTAGACCTGACGGCGTCATCCACGGAATCGAACGCCTCCTCATTCCACGCTCCGTTCAAGACGACTTCAACCGTCGCAGAAGTCTCGTCTCCATCGCCGCAATTAAACCGGAGGGAGCACCGGAAGTTGATCCGAGAACTCACCGCTTGAAAAAACCATCACCGCCGCAAAACCCTGGCTCACCGCCTGCTCTTCCTATATACGACGCATTGGCTCCCGGACCGTCACTAGCACCGGCGCCGGCACCAGGACCTGGTGGACCCCACCACCATTTCAACGGCGACGCGCAGGTGAAAGATTTCATCCAAACACTGATTCATTACGGTGGTTACAACGAGATGGCGGATATTCTCGTTAACCTAACGTCGTTAGCGACGGAGATGAGTCGGTTAGTTTCAGAGGGTTACGTTTTAACGGTTCTAGCTCCGAATGACGAAGCCATGGCTAAGTTAACAACGGACCAGTTAAGCGAACCCGGTTCACCCGAACAAATCATGTATTATCACTTAATTCCGGAGTATCAAACCGAAGAGAGTATGTATAATGCTGTTAGAAGATTCGGGAAGGTTCGTTATGATACACTTCGATTGCCTCATAAGGTGGATGCTCAGGAAGCTGATGGGTCGGTTAAATTCGGGCACGGTGACGGGTCGGCTTATTTGTTCGACCCGGATATTTATACGGATGGGAGAATCTCCGTGCAAGGGATTGATGGGGTTTTGTTTCCTCATCAAGAAGAAGAGGAGGTTGTTGATAAGGATGTGACCCGAACCCGGTTGGGTCAACCCGGTAAAGTTGTGGTTAAGCAAAGAAGAGGAAAATTGTTGGAAACAGCTTGCTGGATGCTTGGAACTTTTGGACAGCATTCTAGATTCACCTCTTGTCAATAAACAAAAAAATGCACATGAAATTCCAAAGATGTAAAAAGTCAAAGGAATATGAATGAAGGCCAGATTTACTTGAATGGTTAATAGCAGCTGCTGCTATATTAGTTTGGNTTTNGGTTTACNCCCTTTAACTTTTTTTCTCTCTYCTTCCTT
TARGET=15,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03961 Ps_contig_mira-and-tgicl-ass_franssen_31588
SEQUENCE=GGGACAAGCACATTACACAACACAACACAACACACAAACATAAACAGAGGTAGCGTTTGTATTCAGTTATATTATTGTTCTTGTCTGAGAAGAACAAAACCTGAAAATGGCCATGGATTGCGGCATCTATGGCGTCATCAAGCTCCGCACGTTCTTCACTTTCCTCATCCTCTTTCTCTCTACCTCCACCTTTTCCCTCTCATCTTCTTCTTCTTCAAATGTTCAAATAAACTCAAACTCAATCTTGGTGGCTCTTCTCGACTCACATTACACAGAACTCGCTGAACTCGTTGAAAAAGCCATGCTTCTTCAAACACTAGAAGACACTGTCGGCAACAGCAACATCACCATTTTCGCACCCAAAAACGAAGCTCTTGAACGAGATCTCGACCCTGACTTCAAAACGTTCCTCCTCGAACCACGAAATCTCAAGTCTCTTCAAACTCTCCTCATGTCACACATCATTCCAACGCGAATCAACGGATCCGAACCCGGGTCGACCCGACACAAAACGCTTTGCCTTCAACACCACCTCACTATCCAACCAAACGAAACTTCTCAACAATGGACAGTTAACGGCGCTAAGATTCTCCATCTAAACGCCGTCACTAGACCTGACGGCGTCATCCACGGAATCGAACGCCTCCTCATTCCACGCTCCGTTCAAGACGACTTCAACCGTCGCAGAAGTCTCGTCTCCATCGCCGCAATTAAACCGGAGGGAGCACCGGAAGTTGATCCGAGAACTCACCGCTTGAAAAAACCATCACCGCCGCAAAACCCTGGCTCACCGCCTGCTCTTCCTATATACGACGCATTGGCTCCCGGACCGTCACTAGCACCGGCGCCGGCACCAGGACCTGGTGGACCCCACCACCATTTCAACGGCGACGCGCAGGTGAAAGATTTCATCCAAACACTGATTCATTACGGTGGTTACAACGAGATGGCGGATATTCTCGTTAACCTAACGTCGTTAGCGACGGAGATGAGTCGGTTAGTTTCAGAGGGTTACGTTTTAACGGTTCTAGCTCCGAATGACGAAGCCATGGCTAAGTTAACAACGGACCAGTTAAGCGAACCCGGTTCACCCGAACAAATCATGTATTATCACTTAATTCCGGAGTATCAAACCGAAGAGAGTATGTATAATGCTGTTAGAAGATTCGGGAAGGTTCGTTATGATACACTTCGATTGCCTCATAAGGTGGATGCTCAGGAAGCTGATGGGTCGGTTAAATTCGGGCACGGTGACGGGTCGGCTTATTTGTTCGACCCGGATATTTATACGGATGGGAGAATCTCCGTGCAAGGGATTGATGGGGTTTTGTTTCCTCATCAAGAAGAAGAGGAGGTTGTTGATAAGGATGTGACCCGAACCCGGTTGGGTCAACCCGGTAAAGTTGTGGTTAAGCAAAGAAGAGGAAAATTGTTGGAAACAGCTTGCTGGATGCTTGGAACTTTTGGACAGCATTCTAGATTCACCTCTTGTCAATAAACAAAAAAATGCACATGAAATTCCAAAGATGTAAAAAGTCAAAGGAATATGAATGAAGGCCAGATTTACTTGAATGGTTAATAGCAGCTGCTGCTATATTAGTTTGGNTTTNGGTTTACNCCCTTTAACTTTTTTTCTCTCTYCTTCCTT
TARGET=203,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03962 Ps_contig_mira-and-tgicl-ass_franssen_31593
SEQUENCE=TTAGCAAAAAAATCAAAAACCTGTGGATTGATCAAGACTTGTTCATAAAATCATATACAGTTAATTGCACGTTGATCAAAACAATACTTTATATAGTACACCATATAACGCATAGCGAATGAGCCAAGGTACAAGAGAATATTGAACTTTATGCTACATCATTTGTAAACAAAAAGAAAATTCATTTTTGATGAATCAATCTTTCCATACAGGAATAATCCCCTGTGCTGCCAAACGTTTTTCAACCCAAAAGACTATGTGTTTTGAAGTGATTTTCTTCTCATCAATGAGAAACGGTTCAACCGTGCTTGCTCCTGAGTTATAAGATGACAGTGAAAACCCCTTTGGACCAGTAATTCCTGTGGGAAAAAAGGCCCAAAATGAATAGGGATTGCCTTTTTTCAGAAATGAACCTTCCAACTTATTGTCCTGAAAAACCAACTCAATATCAGAGAGATCCTTATTGGATTCTAAGATTGATTTCAATGAAGGAATCACATCCTCTTCCATCATCTGAGGTAAAGGCTTAACCGGAGCTTTAGCAGGTGGAGCCTTCTTCTCTGTTGCTACTTTTGGAGCTGCTGCTGCTGCTGCCTTTACCTCCCCCTTTGTTTCAGCAGTAGCTGATGTACTAGTAGTAGATGATTCTTGAACCGCAGAGCAAATTATCTTGTTTCTATTTACCGTTTTAAATCTACTTGCAATCAAAGGTAGTATTGGTGATGACGATGAGTTCGGTATGACAACATTTTGGGAGACATGTAAAGAAGATGAGGTTGAAGTTAAAGAACACAAACGAAAGGTAGAATCTCCAAGTGCTCCACCTCTTAACAAAAGTGCCATTTGCATTATGATTCTTCCTGTGTTTTTTTCTTGTTATTTTTTATTCCATTTTCTGTCACTGGTTTGTTGATGATGTTAGTGAGAAGCATACGTTGAAGGAACAAAATGAATGAACCTTCCCCGCCG
TARGET=578,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0730
PRIMER_LEFT_PENALTY=0.072951
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=TCACATCCTCTTCCATCATCTG
PRIMER_RIGHT_SEQUENCE=TTGTCATACCGAACTCATCGTC
PRIMER_LEFT=494,22
PRIMER_RIGHT=745,22
PRIMER_LEFT_TM=60.073
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.0956
PRIMER_LEFT_1_PENALTY=0.095574
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=TCCATCATCTGAGGTAAAGGCT
PRIMER_RIGHT_1_SEQUENCE=TTGTCATACCGAACTCATCGTC
PRIMER_LEFT_1=505,22
PRIMER_RIGHT_1=745,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.1109
PRIMER_LEFT_2_PENALTY=0.072951
PRIMER_RIGHT_2_PENALTY=0.037936
PRIMER_LEFT_2_SEQUENCE=TCACATCCTCTTCCATCATCTG
PRIMER_RIGHT_2_SEQUENCE=TCATACCGAACTCATCGTCATC
PRIMER_LEFT_2=494,22
PRIMER_RIGHT_2=742,22
PRIMER_LEFT_2_TM=60.073
PRIMER_RIGHT_2_TM=59.962
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.1335
PRIMER_LEFT_3_PENALTY=0.095574
PRIMER_RIGHT_3_PENALTY=0.037936
PRIMER_LEFT_3_SEQUENCE=TCCATCATCTGAGGTAAAGGCT
PRIMER_RIGHT_3_SEQUENCE=TCATACCGAACTCATCGTCATC
PRIMER_LEFT_3=505,22
PRIMER_RIGHT_3=742,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.962
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.1451
PRIMER_LEFT_4_PENALTY=0.145042
PRIMER_RIGHT_4_PENALTY=0.000037
PRIMER_LEFT_4_SEQUENCE=GTAAAGGCTTAACCGGAGCTTT
PRIMER_RIGHT_4_SEQUENCE=TTGTCATACCGAACTCATCGTC
PRIMER_LEFT_4=518,22
PRIMER_RIGHT_4=745,22
PRIMER_LEFT_4_TM=60.145
PRIMER_RIGHT_4_TM=60.000
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_03963 Ps_contig_mira-and-tgicl-ass_franssen_31600
SEQUENCE=GGGGGCTAGTTCTCTTCTCTTCCTTCATTCCGTGCTCTCCGCCTGAAAGTTAAACACTCGAACTCGTGTTTTTTTTTCCGGTGCTCGTTCTTCACCTTCGGATCTATAGTATCTTACATAACACAAAAATAAAAAACCTAAAAAAAAATACAGAATTCAGCGTTTGGTTTCAGTTCTTTCTTTTTTATTCAGGTAGATCCGAAGTGTGAGCATTCAAGATCGAAGGGTAGCTGTTGAAGAATGGCAACAACAGCAGCTGGGAACTCGGCATGGTATAAGGCAAAGGTGAAGGCTGTTACTTCTGGGGACTGCGTTGTTGTTGTTTCCGTGGCAGCCAATGCTAAGTCTGGTGTTCTTCCAGAAAAATCCATCACTTTGTCGTCTTTGATTGCGCCGAGATTGGCGCGTAGRGGTGGTGTTGATGAGGCATTTGCATGGGAAAGTAGAGAGTTTCTGAGGAAGCTTTGCRTTGGAAAGGAGATTACTTTCAGAATTGATTACACCGTGCCATCAATAAACAGGGAGTTTGGAACGGTTTTTCTTTGGCGACAAAAATGTTGCAATGCTGGTTGTTTCTCAARGATGGGCAAAGGTTAGAGAGCAGGGACAGCAGAAAGGAGAAGTGAGTCCATTCCTGGCAGAGTTGTTACGGCTGGAAGAGCAAGCTAAACAAGAAGGCCTTGGTCGTTGGAGCAAGGTTCCAGGTGCTGCCGAGGCATCCATCAGAAATCTACCACCATCTGCCCTTGGTGATGCTAGCAACTTTGATGCAATGGGTTTGTTAGCTAAAAGTAAGGGAGTACCAATGGAGGCACTTGTTGAGCAGGTTCGTGACGGAAGTACTCTCCGAATCTATTTGCTTCCGGAGTTTCAGTTTGTCCAAGTTTTCGTTGCTGGAATCCAGTCCCCACAAATGGGAAGGAGAGCTGCACCTGAAACTGTTGTTGAACCTGAGGTAACAGTTGATAGCACTAATGGAGATGYCCCTGCAGAACCTCGAGCTCCACTTACATCTGCCCAAAGACTTGCTGTTTCAGCATCTGCTGCTGAAACTTCAGCTGATCCTTTTGGACCGGATGCTTAAATTTTTCACTGAGATGCGTGTATTGAATCGAGATGTTCGCATTGTTCTTGAAGGTGTTGATAAGTTCAGCAATTTGATTGGCTCAGTGTATTATCCTGATGGTGAATCAGCAAAGGACTGGCCATTGGAGCTTGTTGAAAACGGCTTTGCTAAATATGTTGAATGGAGTGCAAACATGATGGAAGAAGATGCAAAGCGGAAGCTGAAGTCTGCAGAGCTCGAGGCTAAGAAAAGTAGGTTAAGAATTTGGACAAACTATGTGCCACCGGTTTCAAATTCCAAGGCAATTCATGACCAGAATTTTACTGGCAAGGTGGTTGAGGTTGTGAGTGGAGATTGCGTCATTGTTGCTGATGATTCTATTCCATATGGCAGCCCACAAGCTGAGAGGCGAGTTAACCTTTCAAGTATTAGGTGTCCAAAAATGGGCAATCCTCGTCGAGATGAAAAACCAGCTCCCTATGCACGTGAAGCAAAGGAATTCTTGAGAACACGCCTCATCGGTCGTCAAGTGAATGTACAAATGGAATATTCTAGGAAGGTTGGTCCAGTTGATGCAGCTGGAGCTCCATTGGGAGCTGGTGATAGAGTAATGGATTTTGGATCAGTCTTCCTATTGTCCTCTGGGAAAGCTGACAATGACCAAGCCCCTTCAGCTGCTGCTCCTGCAAGTCAGCAAACTGGGGTTGAATGTCGGCGAATTGGTAATTGGTCGTGGCTTTGGAACTGTCATTAGACATCGTGACTTCGAAGAGAGATCTAACTTCTATGATGCTCTTCTTGCTGCTGAATCACGTGCCATTTCTGGAAGGAAAGGGATCCATTCTGCCAAGGATCCTCCAGTGATGCATATAACTGACTTGACCACGGCATCGGCCAAGAAAGCCAAAGATTTCATGCCTTTCCTGCATAGAAGTAGAAGAGTTCCTGCTGTAGTGGAATATGTCCTCAGTGGTCACCGTTTCAAGTTGTTGATTCCTAAAGAAACTTGCAGTATTGCTTTTGCATTCTCTGGTGTCAGATGTCCTGGTCGTGAAGAGCCATATTCTGATGAAGCAATTGCACTGATGAGGCGGAGGATAATGCAAAGAGATGTTGAGATTGAGGTAGAAACTGTCGATAGAACTGGAACATTCTTGGGATCCTTATGGGAGTCGAAAACAAACGGGGCGGTTGCACTACTCGAAGCCGGTCTTGCAAAACTACAAACCTCCTTTGGCAGTGACAGGATTCCTGATCTTCGTGTCTTAGAGCAAGCCGAACAATCTGCTAAGAGTAAAAAGCTGGAAAATCTGGGAGAATTTTGTTGAAGGGGAGGTAGTACCCAGTGGTGCAAATGTTGAAACCAAACAGCAAGAAGTACTTAAGGTAACTGTTACAGAAGTCTTGGGAGGTGGTAAATTTTATGTCCAGACTGTTGGAGATCAAAAGATAGCATCCATTCAGAACCAACTAGCTTCTTTGAACCTGAAGGAAGCTCCTGTGATTGGTGCTTTTAATCCTAAGAAGGGTGACATAGTCCTCTGTTATTTCCGCGCTGATACGTCTTGGTATCGGGCAATGGTTGTCAATACGCCTCGAGGACCAGTTGAATCTTCTAAGGATGTGTTTGAAGTATTCTACCTTGATTATGGAAATCAAGAAGAAGTACCATACAGTCAACTGAGGCCTCTTGATCCATCTGTGTCATTAGCACCCGGCCTTGCTCAATTGTGTAGCCTTGCATACATTAAGATACCAAACTTGGAAGAGGATTTTGGTCAAGAAGCAGCTGAATACTTGAGTGAACTCACTTTAAGCAGTGGAAAAGAATTTAGGGCCATGGTTGAGGAGAGAGACACTACCGGAGGAAAAGTGAAAGGGCAGGGAACTGGACCAGTTATTGCTGTAACTCTTGTTGCTGTCGATGCTGAGATCAGTGTCAATGCTGCCATGCTTCAGGAAGGACTTGCTAGGATGGAAAAAAGGAACAGGTGGGATAAATCAGCCAGAAAGCAGGCCCTTGATAATTTGGAAATGTTCCAAGGAGAGGCAAGAACCAGCAGGCGTGGAATATGGCAGTATGGAGATATTCAGTCTGATGACGAGGACACTGCTCCTCCACGAAAACCCGCTGGTGGCCGCAGATAAGTGTAACGGGATGAATAAGTTATAAAATATATAGACATCTTGAGAGAGAAAATGATTCAAGTTATAGACATGAGTTAGAGAGAAAAAGAAGAAAATGCCGTGTATTTCCTTTGTAAGTTTTGTTTTGTTTTTGTTTTAACTTTTATTGTTAGATTTGGCTGAACAATTTTTTCCATAGATGTCATAATAAGGTACATGCGGCAGGGTTCTTTTTTAAAAAGACGGCAGAGATGTTTTGATTATTTAAGAAACTGTGTCTTTCCTTTTTTACTGTGGTGTTGTTTGGTTTCGG
TARGET=313,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03964 Ps_contig_mira-and-tgicl-ass_franssen_31600
SEQUENCE=GGGGGCTAGTTCTCTTCTCTTCCTTCATTCCGTGCTCTCCGCCTGAAAGTTAAACACTCGAACTCGTGTTTTTTTTTCCGGTGCTCGTTCTTCACCTTCGGATCTATAGTATCTTACATAACACAAAAATAAAAAACCTAAAAAAAAATACAGAATTCAGCGTTTGGTTTCAGTTCTTTCTTTTTTATTCAGGTAGATCCGAAGTGTGAGCATTCAAGATCGAAGGGTAGCTGTTGAAGAATGGCAACAACAGCAGCTGGGAACTCGGCATGGTATAAGGCAAAGGTGAAGGCTGTTACTTCTGGGGACTGCGTTGTTGTTGTTTCCGTGGCAGCCAATGCTAAGTCTGGTGTTCTTCCAGAAAAATCCATCACTTTGTCGTCTTTGATTGCGCCGAGATTGGCGCGTAGRGGTGGTGTTGATGAGGCATTTGCATGGGAAAGTAGAGAGTTTCTGAGGAAGCTTTGCRTTGGAAAGGAGATTACTTTCAGAATTGATTACACCGTGCCATCAATAAACAGGGAGTTTGGAACGGTTTTTCTTTGGCGACAAAAATGTTGCAATGCTGGTTGTTTCTCAARGATGGGCAAAGGTTAGAGAGCAGGGACAGCAGAAAGGAGAAGTGAGTCCATTCCTGGCAGAGTTGTTACGGCTGGAAGAGCAAGCTAAACAAGAAGGCCTTGGTCGTTGGAGCAAGGTTCCAGGTGCTGCCGAGGCATCCATCAGAAATCTACCACCATCTGCCCTTGGTGATGCTAGCAACTTTGATGCAATGGGTTTGTTAGCTAAAAGTAAGGGAGTACCAATGGAGGCACTTGTTGAGCAGGTTCGTGACGGAAGTACTCTCCGAATCTATTTGCTTCCGGAGTTTCAGTTTGTCCAAGTTTTCGTTGCTGGAATCCAGTCCCCACAAATGGGAAGGAGAGCTGCACCTGAAACTGTTGTTGAACCTGAGGTAACAGTTGATAGCACTAATGGAGATGYCCCTGCAGAACCTCGAGCTCCACTTACATCTGCCCAAAGACTTGCTGTTTCAGCATCTGCTGCTGAAACTTCAGCTGATCCTTTTGGACCGGATGCTTAAATTTTTCACTGAGATGCGTGTATTGAATCGAGATGTTCGCATTGTTCTTGAAGGTGTTGATAAGTTCAGCAATTTGATTGGCTCAGTGTATTATCCTGATGGTGAATCAGCAAAGGACTGGCCATTGGAGCTTGTTGAAAACGGCTTTGCTAAATATGTTGAATGGAGTGCAAACATGATGGAAGAAGATGCAAAGCGGAAGCTGAAGTCTGCAGAGCTCGAGGCTAAGAAAAGTAGGTTAAGAATTTGGACAAACTATGTGCCACCGGTTTCAAATTCCAAGGCAATTCATGACCAGAATTTTACTGGCAAGGTGGTTGAGGTTGTGAGTGGAGATTGCGTCATTGTTGCTGATGATTCTATTCCATATGGCAGCCCACAAGCTGAGAGGCGAGTTAACCTTTCAAGTATTAGGTGTCCAAAAATGGGCAATCCTCGTCGAGATGAAAAACCAGCTCCCTATGCACGTGAAGCAAAGGAATTCTTGAGAACACGCCTCATCGGTCGTCAAGTGAATGTACAAATGGAATATTCTAGGAAGGTTGGTCCAGTTGATGCAGCTGGAGCTCCATTGGGAGCTGGTGATAGAGTAATGGATTTTGGATCAGTCTTCCTATTGTCCTCTGGGAAAGCTGACAATGACCAAGCCCCTTCAGCTGCTGCTCCTGCAAGTCAGCAAACTGGGGTTGAATGTCGGCGAATTGGTAATTGGTCGTGGCTTTGGAACTGTCATTAGACATCGTGACTTCGAAGAGAGATCTAACTTCTATGATGCTCTTCTTGCTGCTGAATCACGTGCCATTTCTGGAAGGAAAGGGATCCATTCTGCCAAGGATCCTCCAGTGATGCATATAACTGACTTGACCACGGCATCGGCCAAGAAAGCCAAAGATTTCATGCCTTTCCTGCATAGAAGTAGAAGAGTTCCTGCTGTAGTGGAATATGTCCTCAGTGGTCACCGTTTCAAGTTGTTGATTCCTAAAGAAACTTGCAGTATTGCTTTTGCATTCTCTGGTGTCAGATGTCCTGGTCGTGAAGAGCCATATTCTGATGAAGCAATTGCACTGATGAGGCGGAGGATAATGCAAAGAGATGTTGAGATTGAGGTAGAAACTGTCGATAGAACTGGAACATTCTTGGGATCCTTATGGGAGTCGAAAACAAACGGGGCGGTTGCACTACTCGAAGCCGGTCTTGCAAAACTACAAACCTCCTTTGGCAGTGACAGGATTCCTGATCTTCGTGTCTTAGAGCAAGCCGAACAATCTGCTAAGAGTAAAAAGCTGGAAAATCTGGGAGAATTTTGTTGAAGGGGAGGTAGTACCCAGTGGTGCAAATGTTGAAACCAAACAGCAAGAAGTACTTAAGGTAACTGTTACAGAAGTCTTGGGAGGTGGTAAATTTTATGTCCAGACTGTTGGAGATCAAAAGATAGCATCCATTCAGAACCAACTAGCTTCTTTGAACCTGAAGGAAGCTCCTGTGATTGGTGCTTTTAATCCTAAGAAGGGTGACATAGTCCTCTGTTATTTCCGCGCTGATACGTCTTGGTATCGGGCAATGGTTGTCAATACGCCTCGAGGACCAGTTGAATCTTCTAAGGATGTGTTTGAAGTATTCTACCTTGATTATGGAAATCAAGAAGAAGTACCATACAGTCAACTGAGGCCTCTTGATCCATCTGTGTCATTAGCACCCGGCCTTGCTCAATTGTGTAGCCTTGCATACATTAAGATACCAAACTTGGAAGAGGATTTTGGTCAAGAAGCAGCTGAATACTTGAGTGAACTCACTTTAAGCAGTGGAAAAGAATTTAGGGCCATGGTTGAGGAGAGAGACACTACCGGAGGAAAAGTGAAAGGGCAGGGAACTGGACCAGTTATTGCTGTAACTCTTGTTGCTGTCGATGCTGAGATCAGTGTCAATGCTGCCATGCTTCAGGAAGGACTTGCTAGGATGGAAAAAAGGAACAGGTGGGATAAATCAGCCAGAAAGCAGGCCCTTGATAATTTGGAAATGTTCCAAGGAGAGGCAAGAACCAGCAGGCGTGGAATATGGCAGTATGGAGATATTCAGTCTGATGACGAGGACACTGCTCCTCCACGAAAACCCGCTGGTGGCCGCAGATAAGTGTAACGGGATGAATAAGTTATAAAATATATAGACATCTTGAGAGAGAAAATGATTCAAGTTATAGACATGAGTTAGAGAGAAAAAGAAGAAAATGCCGTGTATTTCCTTTGTAAGTTTTGTTTTGTTTTTGTTTTAACTTTTATTGTTAGATTTGGCTGAACAATTTTTTCCATAGATGTCATAATAAGGTACATGCGGCAGGGTTCTTTTTTAAAAAGACGGCAGAGATGTTTTGATTATTTAAGAAACTGTGTCTTTCCTTTTTTACTGTGGTGTTGTTTGGTTTCGG
TARGET=3331,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03965 Ps_contig_mira-and-tgicl-ass_franssen_31602
SEQUENCE=CAACGCAGAGTACNCGACAGCACTTCAAACTTACCCCAAACCAAAACAACACTCTCATTTTCCATATCCACCGCCGCACATAGCGGCGCCACCGTAAACTACCGACTAATTTATTATTTATCATAATGGCGGCTTCAACAACCACCGACGGTCCCGTCTTGAACCTAATCAACAAACGCCTCCGTGCTCTCCGGAAGAAACTCAACCGAATCACATCCATGGAGGAATCAGTTTCTCAAGGGAAGCCACTTAACAAGGAACAAGAAGAAGTTCTCCGTTCAAAACCCTCCGTTGTCGTTCTCATTGATGAGTTGGAGAAGCTTCGTCAACCGCTTTCCGCTGCCCTAACTGAAGAACTCGACCTCGCTCTTCAGAACAGCCGCGAAACCCTACCTGAGAATTCCGTTCCGACCGGCCAGAATTCCCCTCCGAATTCGGAACATCGGCCGAACCAGAACGGGGATGTGGTTGAGGATATTCTCAATTTGCTCTACTTTGGTTCGCTTTTCGAAGTGAAGACGCAAAATGATTTTACTTCTACTATGCTTACGAGGACGCATGAGCGTGGATGTTGCTTGACTTATGATTATGTTACTGATGATGCAACCGATCTGCTTGGGGAGAAGGATTTGGACTCGATCTCTGCGTTGTTTGGGTTGTTGATTTCTCGGCCTGCCGATTCAAGCTTTTCGCATAAGAACGCTCTTCGGAGGTGCATTGAGCATGCTAAGTTGTGGGTTTCTAGGGCTCAGCAGCCTATTGACCCCAATGTTGATGTTACATATGCTGGTTTGAGGGAGAAGTTGGACAAAATTATGTCTTCGGAGTACTTTACTACTACTCCTGAGATGAAGGCTCCAGTTGATGTTGCTGCCGCAGCGGCTGGAAATTATGGGTCTTTTCAGGTGCCCGTGCATGATTCCGTGGTTTCTGTTGAAGTAGAAGGTTCTGATTATCAGCATGAGGAGAAGGACGAGAGAGCTGCAAATTTTCAAGGACAAGGATCTGGAGATGATCCGTCTGATCCCGAAGGAGAGTTCCAGAAGGATGAAGTAGAGGCAGAAAATGCAGTTGAGGTGGCATCAGTCCAACACGAGCAGGCTAATTCTCAGGTTGACACGGAATACAATCAAGCAGATGGAGAGGGAAAGGAGCAACAGAATTACCCAAGGAGGGGGGGTTATCAGAATCAAAGAGGTGGACGCGGTGGTGGTGGTGGTAGGAGAGGTTATCCAAATGGCCGTGGAGGTCGTGGTGGAGGCAGAGGAGGGTTTCCAAATGGCCGCAACCAATTCTATGACCAGCCTGGAAATTATTATCCCAGAAACTATTATAACAACAGAGGACGCGGAGGCAGAGGAGGTGGCTACTACAGCAACAACGGCGCAGGCGGTCAGGTTAATCATGTTGCAGGTGATGTTGGGGTGCAATCTTGATAAATTATGACTTGTTCATAGTGTTTGTTACTATAATTGGGTGGGGTGTAACTTTTTTATCGTCTGGCATGAAACNTTGTTTGAACTAATTTTGTTGAACTTCCGAAATCCAGACTCCGCTATTTTGAGTCCAGTAGCGTAAAGTTTAACCTTTACACCCCAAA
TARGET=1206,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0348
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=AAGGAGCAACAGAATTACCCAA
PRIMER_RIGHT_SEQUENCE=AATTTCCAGGCTGGTCATAGAA
PRIMER_LEFT=1148,22
PRIMER_RIGHT=1313,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.0348
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=AAAGGAGCAACAGAATTACCCA
PRIMER_RIGHT_1_SEQUENCE=AATTTCCAGGCTGGTCATAGAA
PRIMER_LEFT_1=1147,22
PRIMER_RIGHT_1=1313,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.0373
PRIMER_LEFT_2_PENALTY=0.003166
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=TTGACACGGAATACAATCAAGC
PRIMER_RIGHT_2_SEQUENCE=AATTTCCAGGCTGGTCATAGAA
PRIMER_LEFT_2=1113,22
PRIMER_RIGHT_2=1313,22
PRIMER_LEFT_2_TM=60.003
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.0519
PRIMER_LEFT_3_PENALTY=0.017846
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=TAGAGGCAGAAAATGCAGTTGA
PRIMER_RIGHT_3_SEQUENCE=AATTTCCAGGCTGGTCATAGAA
PRIMER_LEFT_3=1053,22
PRIMER_RIGHT_3=1313,22
PRIMER_LEFT_3_TM=60.018
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.0644
PRIMER_LEFT_4_PENALTY=0.030276
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=CTCAGGTTGACACGGAATACAA
PRIMER_RIGHT_4_SEQUENCE=AATTTCCAGGCTGGTCATAGAA
PRIMER_LEFT_4=1107,22
PRIMER_RIGHT_4=1313,22
PRIMER_LEFT_4_TM=60.030
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_03966 Ps_contig_mira-and-tgicl-ass_franssen_31606
SEQUENCE=CAACGCAGAGTACGCGGGGAGGTGCAGGAAATTTTCAAATCTCCATTTTCAATATCCTTTACCTGCATCTGTTTTTCAATTTTCATATATCATTTTCATTTTCTCCCTGAAAATCATTCAAACAAATCAAGATAATATCTATTGAAATTGAATACCATTCATCTGGGAAAGAAGAAAATGCCGTCATCGTTGGAGGATGAACTCTTCCCATCAACACCAGGAAAGTTCAAGATCGAGAGAAATCACGGCATGATGAACCGGCAAGTCTACCGTTGTTTCGCTTCCACCAGCACTATGTTCTTGTGGGCCCTTTTTCTCATCGCTCTAACTGCATCTTATCTAAGTTTCCAAAGCTTCGTCGATTCCGGTAGCCGCTATATTTCCGCCTCTTGGGGCGGAATCCAGTGGGAGAAACAAGTTCGAACCTCCGCTCAAATCCACCGGCAAGGTGGCATGTCGGTTCTCGTCACCGGCGCAGCAGGTTTCGTTGGGTCCCACGTCTCGCTCGCTTTAAAAAGAAGAGGAGACGGCGTCATTGGACTGGATAACTTCAACGACTATTACGATCCTTCACTTAAAAAAGCTCGTAAAGCTCTTTTAACAAGCCACGGCGTTTTCATCGTGGAAGGTGATATAAACGACGCTAAGCTATTAGCCAAGCTATTCGATGTTGTGGCTTTCACTCATGTTATGCATCTCGCGGCTCAAGCTGGTGTTCGTTACGCAATGGAGAATCCTCACTCTTATGTCCACAGCAATATAGCCGGACTCGTCACGCTTCTCGAAGCTTGTAAAACGGCTAACCCTCAGCCGTCTGTTGTTTGGGCTTCGTCAAGCTCGGTTTACGGTTTAAACGAGAAATCTCCGTTCTCCGAATCAGATCGGACCGATCAACCGGCGAGCCTATACGCGGCGACGAAAAAGGCCGGCGAGGAAATTACTCACACCTACAATCACATCTACGGTCTCTCCATCACCGGATTACGGTTCTTCACCGTTTACGGTCCTTGGGGAAGACCTGACATGGCTTATTTCTCGTTCACCCGGAACATTCTCCAGGGAAAGCCTATAACGGTTTACCGCGGCAAGAATCACGTCGATCTAGCTCGTGATTTCACATACATTGATGATATCGTGAAAGGTTGTATCGGATCGTTGGATACTTCAGGGAAGAGCACTGGATCCGGTGGTAAGAAACGAGGACCCGCACCTTACCGGATTTTCAACCTTGGAAATACTTCTCCGGTGACTGTTCCGACGTTGGTGAGTATTTTAGAACGGCATTTGAAGGTGAAGGCGAAGAGGAATATTTTGGATATGCCTGGAAACGGCGACGTTCCGTTTACACATGCGAATATTAGTTCGGCGCGAAGAGAACTCGGATATAAACCCACAACCGATTTACAAACCGGGTTGAAGAAATTCGTGAAATGGTATCTTTCATATTACGGATATGGCAAACCTGTAAATTAATTTGTTTTTTTTAAATCTTTTTAGTCGGTTAGAATTTTTAATTAAGGTGGATGTAAAAATACTATACGGGAAGAAGAAAAGAAGAATAGAAAGAATCCCGGTGAAGAAGAAAAGGGAGAGAAAGGAAAAAACAAAGGAAAGAAGAAAGAAAGAAGAAAGAATCCAATTCTTTATTCTTTACTTCATATGAGGTTACAGCGAAATCATAATACAGCTGTTTTCCTTAGATTATTTCTTTTCTTTTTTTGTTTATGTTAATTTATTATTTTACATTTTTTTTCCCTCATATGTAAAGAAAGTTGAAGTTAATTTTGTCTCCTTTAATGGTAAGTTTAAATATTTGAATTAACGG
TARGET=1615,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.3451
PRIMER_LEFT_PENALTY=0.068302
PRIMER_RIGHT_PENALTY=3.276815
PRIMER_LEFT_SEQUENCE=TAGAAAGAATCCCGGTGAAGAA
PRIMER_RIGHT_SEQUENCE=GCTGTATTATGATTTCGCTGTAACC
PRIMER_LEFT=1559,22
PRIMER_RIGHT=1687,25
PRIMER_LEFT_TM=60.068
PRIMER_RIGHT_TM=60.277
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=129
PRIMER_PAIR_PENALTY_1=3.3721
PRIMER_LEFT_1_PENALTY=0.095324
PRIMER_RIGHT_1_PENALTY=3.276815
PRIMER_LEFT_1_SEQUENCE=CGGATATGGCAAACCTGTAAAT
PRIMER_RIGHT_1_SEQUENCE=GCTGTATTATGATTTCGCTGTAACC
PRIMER_LEFT_1=1448,22
PRIMER_RIGHT_1=1687,25
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=60.277
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=3.8352
PRIMER_LEFT_2_PENALTY=0.558430
PRIMER_RIGHT_2_PENALTY=3.276815
PRIMER_LEFT_2_SEQUENCE=GAATAGAAAGAATCCCGGTGAA
PRIMER_RIGHT_2_SEQUENCE=GCTGTATTATGATTTCGCTGTAACC
PRIMER_LEFT_2=1556,22
PRIMER_RIGHT_2=1687,25
PRIMER_LEFT_2_TM=59.442
PRIMER_RIGHT_2_TM=60.277
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=3.8723
PRIMER_LEFT_3_PENALTY=0.595491
PRIMER_RIGHT_3_PENALTY=3.276815
PRIMER_LEFT_3_SEQUENCE=ACGGATATGGCAAACCTGTAAA
PRIMER_RIGHT_3_SEQUENCE=GCTGTATTATGATTTCGCTGTAACC
PRIMER_LEFT_3=1447,22
PRIMER_RIGHT_3=1687,25
PRIMER_LEFT_3_TM=60.595
PRIMER_RIGHT_3_TM=60.277
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=4.1857
PRIMER_LEFT_4_PENALTY=0.908894
PRIMER_RIGHT_4_PENALTY=3.276815
PRIMER_LEFT_4_SEQUENCE=ATAGAAAGAATCCCGGTGAAGA
PRIMER_RIGHT_4_SEQUENCE=GCTGTATTATGATTTCGCTGTAACC
PRIMER_LEFT_4=1558,22
PRIMER_RIGHT_4=1687,25
PRIMER_LEFT_4_TM=59.091
PRIMER_RIGHT_4_TM=60.277
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_03967 Ps_contig_mira-and-tgicl-ass_franssen_31608
SEQUENCE=TCGGGGACTTATTGCTTTTTATCAATTTGGCTTAACAGTAACAATCAGAGGGAAACCCTTTCTCTCTCTCTCTCTCACACAGATACAAACACTCTGCGAAGCAATCAAACCAAGTAGTAACAATGGGAGATCGAAGCAACGGTGATGTTCTCATGCTGGAGGCTCCACCGTCTTACGGTAGTAGGTCAACCAATTCCGACGCCGAAATCATCGACGCATTGCCTTACATCGACGACGACTACAGCGACCCTAGGGTCAAACTCGAAGTCGACCGTCTCGTCGAAGAAGAGATGCGCCGCAGCTCCAAGAAACCCGCTGATTTCCTCAAAGATTTTCCACCTCTTCCAACTTTCAATTTTCAGGAGTATCCAATGATTGGTAGAGAGTATGAGCGTGTGAGAGCTGGTAGGCCTCCGATTTCACTTGACAGGTCGAGATATGAATTAGAACTACCACCAATTAATAAAAGGAACGATGAAACTGCTTGGAAACTAGCTCTTCAAAGAGCTCAGCGACTACTTCAATATCAAACTATGAGGATGGAAAATTTGGATTTGTTGTTGAAATATGGTCCTGATGCATGGAAACAGCACAACCTGCGATTGGAAGTATACTTGTCAAGAATGCAGAAATTAGCTCAGGAACAAAATGAAAAGATTGAAAAAGTCAACCGAGAAAGGAAATATCATCAGCAAAATACTGCATATGAGCTTAATGCTCTGTCCATCCAATGGAACGAGCTCTGTGTGAAAAATATAAACATCAGAGCTGCGTGCGCTTCAGCTGAAACTCACCTTAATGACCTGAGAAGAGAAGCAACAGAAAGAGGCTGGAACTTGGAGGCTATCACTGAAAATGGGCAATATGCCAATTCGGCATCATGAGATTTGTATCTGCTCATCTAGTTTTGTATCTGTTATTCCAACTTTAATTGCTACTATACCTATTAATTAATATTTAGTTTATTATATCATGAAATTAATTAAACCCATTTGCAATAAACCAAAAAATAATAAACATGTCAAATTATTATTAGATGAAACAGGTTGAAAAGG
TARGET=61,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0034
PRIMER_LEFT_PENALTY=0.951013
PRIMER_RIGHT_PENALTY=0.052366
PRIMER_LEFT_SEQUENCE=CGGGGACTTATTGCTTTTTATC
PRIMER_RIGHT_SEQUENCE=TTGCTTCGCAGAGTGTTTGTAT
PRIMER_LEFT=1,22
PRIMER_RIGHT=103,22
PRIMER_LEFT_TM=59.049
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=103
PRIMER_PAIR_PENALTY_1=1.0034
PRIMER_LEFT_1_PENALTY=0.951013
PRIMER_RIGHT_1_PENALTY=0.052366
PRIMER_LEFT_1_SEQUENCE=CGGGGACTTATTGCTTTTTATC
PRIMER_RIGHT_1_SEQUENCE=ATTGCTTCGCAGAGTGTTTGTA
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=104,22
PRIMER_LEFT_1_TM=59.049
PRIMER_RIGHT_1_TM=59.948
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=104
PRIMER_PAIR_PENALTY_2=1.1406
PRIMER_LEFT_2_PENALTY=0.951013
PRIMER_RIGHT_2_PENALTY=0.189596
PRIMER_LEFT_2_SEQUENCE=CGGGGACTTATTGCTTTTTATC
PRIMER_RIGHT_2_SEQUENCE=TGTAGTCGTCGTCGATGTAAGG
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=242,22
PRIMER_LEFT_2_TM=59.049
PRIMER_RIGHT_2_TM=60.190
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=1.1887
PRIMER_LEFT_3_PENALTY=0.951013
PRIMER_RIGHT_3_PENALTY=0.237699
PRIMER_LEFT_3_SEQUENCE=CGGGGACTTATTGCTTTTTATC
PRIMER_RIGHT_3_SEQUENCE=GGAATTGGTTGACCTACTACCG
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=196,22
PRIMER_LEFT_3_TM=59.049
PRIMER_RIGHT_3_TM=59.762
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=1.2368
PRIMER_LEFT_4_PENALTY=0.999081
PRIMER_RIGHT_4_PENALTY=0.237699
PRIMER_LEFT_4_SEQUENCE=TAACAATCAGAGGGAAACCCTT
PRIMER_RIGHT_4_SEQUENCE=GGAATTGGTTGACCTACTACCG
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=196,22
PRIMER_LEFT_4_TM=59.001
PRIMER_RIGHT_4_TM=59.762
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_03968 Ps_contig_mira-and-tgicl-ass_franssen_31613
SEQUENCE=GGTAAARCTTAAATTGGTAAAAGCATTATGATTATATATACAACCACCCCAGTAATTATTTCAGCTAAAGTAAGAGCTTGATTTCAAAGTAACTAGCCGTTTACCACAAGCTTAATTACAGTAAATAAGAGTAACCATAAATTCCATGCACCAAATTCAATGTACAGGTAGAATATATCACTCTCTACATCCCTTATGTGCCCAAATACAGAATTCAATTTCTTATATAACCCGATCAACATTTGAAACCCCCACAAGCAATAACCCATAATATTATAATTAAGATAAGGATTCCTCCTCCCACCATCAGCTTCATTTGGAGATTCTGCAGCCACATCTTCCTTCTCAGCTGCCTTCCCTGCCTCTGGAAGCTGTCGGCCTGGAACTGCAAGTTTTCGGTTTTATCCACCAGAAGTTCAATCTTCTCTCCACGGTCCAAAACCTTCTCAATGTTATCCATCATTACACCCTTGACCTCAGTTATTTGAGCCTTCAATTTTGATAGTTTACTCATTTCTTCAGGATGGGTCAAACAATACTGCATATGCCCCTTAAGACTTGGCCCAAATTCTCTGTCAAGATTATATGCAATGCTAAACCTATCTTCAAACAAATCATCGTCCTCGTCATCATCAGCAAGTGGATGGTCACTTCCATTCGTAATACTAGAACCATAACGCTGTTTAAAATCATCCTTCACCCGTTCCAGAAATACAAAAGGAACACTCCTTCCAATCGATTCATCAGCAACAACAAGGAAAACAAATCCATTGTCTAAGAGGAAATTAAAGGTGTGACCATCACACGAATACGTGTATTTGGTGCTATTTGAAGGAAGCTTATTCAAACACTGAACAGCAATGGTACTGAAATTTCCTGTATACTGAGTGTGCTCTGCTAAACAACAGTTCCTTTGGCAACAAAGCTATATATCAAACCCCTTTGACTCATCTCCAATTTCCACAACAACAACAAAAAAAAACTACACAGCCAGAAGATACACCACAATCACCACAACAACCTAATCAATCTGAAGTGGAACGAGATCGAGAATCGGTGGTTTGATTCGTTGCGAAAGATGAATTTGATCGAAGAAGAAGCAAGAAAAAGATAGGAAGGTGAAGGTATTAATAACGACAGCGTTTTCAGTTTGATCAGTTACGTCCAGCT
TARGET=963,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03969 Ps_contig_mira-and-tgicl-ass_franssen_31619
SEQUENCE=TTAATTGTAAACTTTCTATTACTTTGGTAGACCCACGTTAGGATATGCAAGGAATATCCTTTAACTAAAATTCTATTTTTTTAGTTGTTCGTGTCATCAAATCCTCTAAATCTCAATATTGTGTTGAATTTAGAGTGGTTGCCAAAATATAAGAGAGAGATACAAGTTATTCAATTCATTAACTTAGTAGTAGTTTATTTACAAAAAAAAAGAATAAAAGAAAAAAACTTTATGTTAATGTTAATTAATAAACTAATTAATCATCTTCATCATCATCTTCCTCGTCATCATGTTCTTGTAGATCCGCAAGAGAAAAGCTTCTTGACTTAAAGCTTCTAAGTCTAAGCCTCATTTCAGCCGCATAAGATTCATGCATTCTACCATGCCCAATCTGATATTGATCTTCTTGTTTCTTATCCTCTCCCAAGGAAGATGAAGACGAAGAGGATGAAACTTTTCTGGTCTTCTCCTCTTCTTCATAATCATAATAATCATCATTATGATCCTCATTAACAGGCAAAAGAGGCATGGATTTAGGGTTTGACCACGAATAGAAAGATGATTTTCTTGAAAATTTGGATGCAATTAGAACCCTTCTTCTCTTGTTAAAAGGACTCTCTTGCTTCCGCAACTCGGTTAAATTGCTTACTTGTGATAGATCTGTGAAGGATTTTGATTTTCCTTCAAAATGACTCGATAATCCCCTCTTGATGGGGAGAGAGTCTTCTAAAGAATCCAACGAACCCAATCCACTTCTTGGCTTCATCTGACTCTGAACTTCCTCATCATTGTCACTGTCATCAGGAGTTCCGATCGACGACGAACTTTCCGGCGACCCGCCGCCGAAAAACTTTGCTGGTCCCGAGATCCGAAACGCTGTCGGAGCTTCATCTTCATTCATGAAGAGGCGGCGGCGGCCGACGGTCTGATCGGAAGGCGGCGGAGATGAAGGAACGTCGATGGTGAAAGTGGGACCCACTAAAACCTCCATTTTAACCCCCAAAAAACTATTTATTTTAAAGAAATTGAATTTGATTACCCTTGTCCTGTTCTTCTTTTCTTTGTCTTGGGTTTCTGAGTAAAAGGGTTATTTGTCATCAAAATTCAGAAAAATTAGAAACAGTAACTATATAACTAAATCAAGACAATGAATGAATTATGAGATTCATTGTTTTGTAACAACTATTAAAAACGTTGGTGTTGTGTTGGTGTTGTTGTTCATGAAAAATGAAAGAAAGAAGGAAGGAAATGAAGTGTGTTCCCCGAAAGTAGAATGATATGATTATATATC
TARGET=907,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8309
PRIMER_LEFT_PENALTY=0.384515
PRIMER_RIGHT_PENALTY=0.446370
PRIMER_LEFT_SEQUENCE=TTCTTGGCTTCATCTGACTCTG
PRIMER_RIGHT_SEQUENCE=AAATGGAGGTTTTAGTGGGTCC
PRIMER_LEFT=753,22
PRIMER_RIGHT=992,22
PRIMER_LEFT_TM=59.615
PRIMER_RIGHT_TM=60.446
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.8309
PRIMER_LEFT_1_PENALTY=0.384515
PRIMER_RIGHT_1_PENALTY=0.446370
PRIMER_LEFT_1_SEQUENCE=CTTGGCTTCATCTGACTCTGAA
PRIMER_RIGHT_1_SEQUENCE=AAATGGAGGTTTTAGTGGGTCC
PRIMER_LEFT_1=755,22
PRIMER_RIGHT_1=992,22
PRIMER_LEFT_1_TM=59.615
PRIMER_RIGHT_1_TM=60.446
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.9688
PRIMER_LEFT_2_PENALTY=0.522443
PRIMER_RIGHT_2_PENALTY=0.446370
PRIMER_LEFT_2_SEQUENCE=TTGGCTTCATCTGACTCTGAAC
PRIMER_RIGHT_2_SEQUENCE=AAATGGAGGTTTTAGTGGGTCC
PRIMER_LEFT_2=756,22
PRIMER_RIGHT_2=992,22
PRIMER_LEFT_2_TM=59.478
PRIMER_RIGHT_2_TM=60.446
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=1.0764
PRIMER_LEFT_3_PENALTY=0.629991
PRIMER_RIGHT_3_PENALTY=0.446370
PRIMER_LEFT_3_SEQUENCE=ATCCACTTCTTGGCTTCATCTG
PRIMER_RIGHT_3_SEQUENCE=AAATGGAGGTTTTAGTGGGTCC
PRIMER_LEFT_3=747,22
PRIMER_RIGHT_3=992,22
PRIMER_LEFT_3_TM=60.630
PRIMER_RIGHT_3_TM=60.446
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=1.2062
PRIMER_LEFT_4_PENALTY=0.759842
PRIMER_RIGHT_4_PENALTY=0.446370
PRIMER_LEFT_4_SEQUENCE=AATCCACTTCTTGGCTTCATCT
PRIMER_RIGHT_4_SEQUENCE=AAATGGAGGTTTTAGTGGGTCC
PRIMER_LEFT_4=746,22
PRIMER_RIGHT_4=992,22
PRIMER_LEFT_4_TM=59.240
PRIMER_RIGHT_4_TM=60.446
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_03970 Ps_contig_mira-and-tgicl-ass_franssen_31624
SEQUENCE=GCTTTAATAAATTAAGGTTTTATTAGACACTTCAACGATAAAATAAGCATGGCCTATAAAACATGGCCTATAAAAAACATGCAGAAAAACAGAGCTCAACTAGGAAAAGAAACTACCGATTATGAGGTTTGATATAATATTTCTCAACTGCATATGTAATAATCTTACATTTGATAAAAACTTTGAACAAAGACATTGATAGTATCCACACGTACACTAACTACAAATCTCTCATATTGGCTGACCTTTGGGAACAATTTGATCTTTGAGCCACATGTAAATAGGAACCAATGTAAACCATGCAGCTGCCAGGACACCCAAAATAAACCGCGGTAAGAAGACAAAAGGATCTACAGGCTTTTCAACATCTTCTTCCTTTGGACCAAGCTGCAAAGGAGAATCCCCAGATGCAGGCTTAACACCAGTCACCGAACAACCCATAATCAAGCCATGACGGCGAACTCCACGGTACCATTTTGGGCCAATCCTTTTTAGTTGGACATTCTTAAACCCTGCCTTTGTAAACCATTCAATATACTCTTCTTCTGTAGGGAAAAGCATCCATACATCTGCAAAGAAACGCGACAACCAAAATGTTGGGTGGACAGGACCAATTAAACATGCCTTTCCCCCAAGTTTCAAAACCCTGTATGCTTCCTTGATGCCACGCTGGGGATCTGGCCAGTATTCAATACTTCCTGCGGATACATATCTATCGGCATAATCAGTTTTAAAGGGAAGATCTTCAGCATCCCCTTCAATTATTTTACAATCTTTAAGCGGTTCCTTTTCCTTAGCTTTAGCAAGCTGATGAGGCGACTGATCAAGAATGGTAACATTCTTAGCATCCACATGCTTAACAATACCCAAAGTGGTAAAACCAGTCCCACCACCAACATCAACAACAAGCATATTCCTATCAGTGAGTTCAGCTGGTTCAAGAGCTTCATCTCTCATATCCTCAGTCCAATGACCAGGGTTTACAACATGGTCATACACAATTGAGAGAAACCTATAAAACCAATACGCCTCTTTTTTGTGTTGTATAAACCTAGGTTGTGAGCTTGGCCTCGAAGACGATGATACACTAGAACTACTACACTTAGGTACTAAACTAGTCCTCGAATATGAAACCCTAGAAGTAGCATTGATACTCGATTTGGGGAAACTTAAGCTCTTACCATGGAAATCTGAAGTTGTGAAACCTAATCCATTTGGGGTTCTGTTATTCAACACTGTGAGCTTTCCGGTTCCACTGAGCATCAGTGAAGCCATTGAATTAACCGAGTGATGGAGAGAGAGAAAGAGGGTGAGAGAGAAACACGTAGCGCAAGCTTTAAACGAAGGTGGGAGTGAAAGGGGAATGTCGTTTTGGGTGTAGAAATGTGTCGCTCACAAAATTTCTAGTTCAATGCATTTCTCGCCACATACTCAAACCTCCGACCG
TARGET=1294,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1311
PRIMER_LEFT_PENALTY=0.029253
PRIMER_RIGHT_PENALTY=0.101896
PRIMER_LEFT_SEQUENCE=CCATGGAAATCTGAAGTTGTGA
PRIMER_RIGHT_SEQUENCE=CACATTTCTACACCCAAAACGA
PRIMER_LEFT=1180,22
PRIMER_RIGHT=1387,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.1311
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.101896
PRIMER_LEFT_1_SEQUENCE=CCATGGAAATCTGAAGTTGTGA
PRIMER_RIGHT_1_SEQUENCE=CGACACATTTCTACACCCAAAA
PRIMER_LEFT_1=1180,22
PRIMER_RIGHT_1=1390,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.1311
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.101896
PRIMER_LEFT_2_SEQUENCE=CCATGGAAATCTGAAGTTGTGA
PRIMER_RIGHT_2_SEQUENCE=CATTTCTACACCCAAAACGACA
PRIMER_LEFT_2=1180,22
PRIMER_RIGHT_2=1385,22
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.1504
PRIMER_LEFT_3_PENALTY=0.048460
PRIMER_RIGHT_3_PENALTY=0.101896
PRIMER_LEFT_3_SEQUENCE=AACCTAATCCATTTGGGGTTCT
PRIMER_RIGHT_3_SEQUENCE=CATTTCTACACCCAAAACGACA
PRIMER_LEFT_3=1202,22
PRIMER_RIGHT_3=1385,22
PRIMER_LEFT_3_TM=59.952
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.1504
PRIMER_LEFT_4_PENALTY=0.048460
PRIMER_RIGHT_4_PENALTY=0.101896
PRIMER_LEFT_4_SEQUENCE=AACCTAATCCATTTGGGGTTCT
PRIMER_RIGHT_4_SEQUENCE=CACATTTCTACACCCAAAACGA
PRIMER_LEFT_4=1202,22
PRIMER_RIGHT_4=1387,22
PRIMER_LEFT_4_TM=59.952
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_03971 Ps_contig_mira-and-tgicl-ass_franssen_31634
SEQUENCE=TGGGGTTCAGGCGACGTGAGTTTAGTAGCGCTACTGCTACCACCTGCATTTCGCTAACGTTCGTTCACCTCTCTCTCTCTCTCTACGGTGGCAATGGAGAGGAGGGGCGACTAAGAAGAAATCACCACCTCTCTCTTTGGACCAATTCATCTCTATCACTGCCCCTCTTCTCGACTTGGAAAAGGAGGCTGAGATATCGAGTTCAATCGCCACTGGTGCATCAAGGAATTTGGATACTGCTCAAAAGAGAGGATCAACAATCCTCAATTTGAAATGTGTGGATGTTCAGACAGGACTTATGGGAAAGTCTCTGATCGAGCTCCAATCCACAAAAGCAGATGTTCTTCCTGCACACAAGTTTGGTACTCATGATGTTGTTGTTTTAAAACTCAACAAGGCTGATTTAGGTTCTCCTGCTCTTGGGCAAGGTGTAGTTTACAGATTAAAGGACTCATCAATTACTGTTGCTTTTGATGATATACCAGAAGATGGTTTAAACAGTCCCCTAAGGCTGGAAAAAGTTGCAAATGAGGTGACGTATCACAGGATGAAAGAGGCATTAGTACAGTTGAGTAAAGGTGTGCATAAGGGTCCTGCTTCTGATCTAATCCCTGTCTTGTTTGGGGAGAGGCAACCAACAGTGTCCAAGAAGGATGTATCTTTCACTTCCATTAATAGAAATCTTGATCACTCTCAGAAAGATGCAATTTCAAAAGCCCTATCGTCGAAGAATGTCTTTTTGCTACATGGACCTCCTGGAACGGGAAAAACTACAACTGTGGTGGAAATTATATTACAAGAAGTGAAACGTGGATCTAAGATTCTCGTTTGTGCTGCCTCAAATATTGCTGTTGACAACATTGTTGAGCGGCTAGTTCCACACAGAGTTAAGCTGGTGAGGATTGGTCATCCTGCACGTTTATTGCCCCAAGTACTGGACAGTGCACTGGATGCTCAGGTACTACAAGGAGATAATAGTGGCCTTGCAAATGACATTCGGAAAGAAATGAAGGTATTGAATGGAAAGCTGCTAAAAACTAAAGAAAAAAATACAAGGAGGGAAATACAGAAGGAACTTAGGACTCTATCTAGAGAAGAGCGGAAAAGGCAGCAGCTTGCTGTTACAGACGTAATTAAAACTGCAGATGTAATATTAACTACTCTGATTGGGGCTTTCACTAAGAAACTTGACCGCACTTCATTTGATTTGGTGATTATCGATGAAGCTGCTCAAGCACTTGAGATAGCATGTTGGATACCTCTGCTAAAGGGTACAAGATGTATCCTTGCAGGAGATCATCTTCAACTTCCTCCAACCATTCAAAGTGTTGAAGCTGAGAAGAAGGGCTTAGGAAGAACCCTCTTCGAACGACTTGCAGAACTGTATGGAGAGGAAGTCACATCTATGCTTACCGTCCAGTATCGGATGCATCAACTTATCATGGATTGGTCTTCTAAAGAGCTTTACAACAGTAAGGTAAAGGCTCATTCAAGCGTTGCTGCACATATGCTATATGATCTTGAGAATGTGAAGAGGACATCTTCAACTGAACCAACCATTCTTCTCATAGATACAACTGGATGTGATATGGAAGAAAAGAAAGATGAAGAGGACAGCACCCTTAACGAAGGAGAATCTGAAGTTGCTATGGCTCATGCAAAGCGATTAGTGCAAAGCGGGGTGCTTCCTTCTGATATTGGAATCATTACGCCTTATGCTGCTCAGGTTGTTTTACTCAAGATGTTGAAAAACAAGGAGAACTCGCTGAAGGATATTGAAATCTCAACGGTTGATGGTTTCCAGGGAAGGGAGAAGGAAGCCATTATTATATCAATGGTTCGATCAAATTCAAAAAAGGAGGTGGGCTTTCTGAGTGATCGCAGGCGAATGAATGTGGCCGTGACACGAGCAAGAAGGCAATGCTGTATTGTCTGTGACACTGAGACGGTCAGTAATGATGGATTTCTAAAGCGGTTGGTTGAGTATTTTGAAGAGCATGGAGAATATCAGAGTGCATCTGAGTACCAGAATTAGTAGGCGAAATTGCCCAAGGTGAAGATTGCTGTTATTGCCCTCATGTGATCAATTTGTCGATTTTGGTGATGTAGATCTGTGAAGGTTTCTAAAATCTTTCTTTTGCTTGGAGGTTAATGTTTTATTGAAGAAAGAAGGCAAGGCCATTTTATTTAGAAGTGAAAATTTATTAGTCTCCATATTATAACATTTAGAATCATCTCGAATTAGAATATTTAGTTTCTTATTATTTYATCTTTTTTATAATTGGTATATGATGCTTATAACATCTACTTTTAATTTAAGTTCTTCTAGT
TARGET=69,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03972 Ps_contig_mira-and-tgicl-ass_franssen_31635
SEQUENCE=CGCGGGGGTTGCTGCTGATTGTATGGTCTGAAAGAACCAATGGCTACTGCGAAGATAGGACTCGGAGTGGCCACTGTTCCCACTCACCGAGTCACCACTGCCGCCGCTCTTTCATCCGCCGCAAGTCCTCCGCCTCACCGTTTCATCTTCATCAATTCACGCCGCTCCCTCCGAACTCGTCTTTCTTCTCTCACACTCCGAGCCTCTTCCGAGACTTCTTCTACCTCCTCCTCCTCTGTGGCCTCACCAGGAAAAGGAGTTGAGAATGTGGTAATTATAGGTTCAGGCCCTGCAGGATACACCGCAGCAATATATGCTGCACGGGCCAATTTGAAACCTGTAGTGTTTGAGGGTTACCAGATGGGTGGTGTTCCTGGGGGTCAATTGATGACTACAACTGAAGTAGAGAACTTTCCAGGATTTCCAGATGGGATTACTGGACCTGATCTAATGGACAGGATGCGACGGCAGGCTGAACGTTGGGGAGCAGAGTTGCATCATGAAGACGTTGAAGCTATTGATGTGAATAGTAGTCCTTTTACTATCCAAAGCAGTGAACGTAAGGTAAAGAGCCATACTGTAATATTTGCTACTGGAGCAACTGCAAAACGACTTAGGCTACCCAGAGAAGATGAATTTTGGAGTCGAGGAATTAGTGCTTGCGCAATTTGCGACGGAGCATCACCTCTCTTTAAGGGTCAAATTCTTGCTGTCGTTGGAGGGGGGGATACAGCTACAGAGGAAGCGTTATACTTGACAAAATATGCTCGTCATGTTCACTTACTTGTACGCCGGGACCAACTAAGGGCTTCTAAAGCCATGCAAGATAGAGTGTATGACAATCCAAATGTCACCGTGCACTTCAATACAGAGGCTGTGGATATTGTAAGCAATACAAAAGGACAAATGTCTGGCATTTTAGTAAGAAAGCTGGATACCGGGGAGGAGTCTGTGCTTGAGGCCAAAGGGCTATTCTACGGCATAGGCCATTCACCAAATACCCAACTGTTGAAAGGCCAAGTTGAACTTGACCAGTCTGGCTACTTGCTTGTTAAGGAGGGCACTGCAAAAACTTCAGTTGAAGGTTTATTTGCTGCTGGAGATGTGCAGGACCACGAATGGAGGCAAGCCGTAACTGCTGCTGGATCTGGATGCATTGCAGCTTTATCTGTTGAGAGATATCTCGTCAGCAGTGGTCTTCTTATAGAGTTCCATCAGCCAAAACATGAAGAGGTTAAGAAGGAACTAACAGACAGGGATGTTCATGCGGGCTTTGACATTACACTTACAAAGCATAAGGGGCAGTATGCTCTGCGGAAATTATATCATGATAGTCCAAGGCTTATATGTGTATTATACACGTCACCAACATGTGGTCCATGTCGAACCCTGAAGCCAATCCTTAGTAAGGTAATTGATGAATATGATCAGAGTGTACATTTTGTTGAAATTGATATAGAGGAAGATCCAGAAATTGCAGAAGCAGCTGGAATCATGGGTACTCCGTGCGTGCAGTTTTTTAAAAACAAGGAGAATCTTAAGACTGTTTCAGGCGTTAAAATGAAGAGAGAATACCGAGAATTCATCGAGGCGAATATATAGAAGTACATAAAAACATTCGAGGGTGATAACCGAATTCCGTGATTCAATTTTGTTTTCCAAATTGGCCAGCTGAATAGTGCTGTCAGTTTTGACAACTATTCCTTGAACCGAGCAGCATTTTCTTAGATTGTACATGTAGAGCTACCAGTTATTGTTGTAATTTTGATTGTTCAATATTACACTTTTCTATTTGAATCCAGCAAGTTCAACTACAAGTTTCTCGTTGACGACAATGTGGCGAGTGAAATGACTCCCATCATGTAATCATCCAAGCTGAAATGACTGCTCATATTTATTATTTTTCACACTTTAATCGGTAAGTCCATGTCAAATTTAATGCAACTTATCCTAGACCTGTTTATACGTAAAACG
TARGET=224,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1564
PRIMER_LEFT_PENALTY=0.150322
PRIMER_RIGHT_PENALTY=0.006089
PRIMER_LEFT_SEQUENCE=AGCCTCTTCCGAGACTTCTTCT
PRIMER_RIGHT_SEQUENCE=TATATTGCTGCGGTGTATCCTG
PRIMER_LEFT=200,22
PRIMER_RIGHT=313,22
PRIMER_LEFT_TM=60.150
PRIMER_RIGHT_TM=60.006
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=0.2325
PRIMER_LEFT_1_PENALTY=0.226380
PRIMER_RIGHT_1_PENALTY=0.006089
PRIMER_LEFT_1_SEQUENCE=ATGGCTACTGCGAAGATAGGAC
PRIMER_RIGHT_1_SEQUENCE=TATATTGCTGCGGTGTATCCTG
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=313,22
PRIMER_LEFT_1_TM=59.774
PRIMER_RIGHT_1_TM=60.006
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.2499
PRIMER_LEFT_2_PENALTY=0.226380
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=ATGGCTACTGCGAAGATAGGAC
PRIMER_RIGHT_2_SEQUENCE=CCACATTCTCAACTCCTTTTCC
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=59.774
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.2501
PRIMER_LEFT_3_PENALTY=0.243965
PRIMER_RIGHT_3_PENALTY=0.006089
PRIMER_LEFT_3_SEQUENCE=AATGGCTACTGCGAAGATAGGA
PRIMER_RIGHT_3_SEQUENCE=TATATTGCTGCGGTGTATCCTG
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=313,22
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=60.006
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.2675
PRIMER_LEFT_4_PENALTY=0.243965
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=AATGGCTACTGCGAAGATAGGA
PRIMER_RIGHT_4_SEQUENCE=CCACATTCTCAACTCCTTTTCC
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=270,22
PRIMER_LEFT_4_TM=60.244
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_03973 Ps_contig_mira-and-tgicl-ass_franssen_31636
SEQUENCE=AGTACGCGGGGATTTTTTTTCATTTCGCATTCTCCTCCTCATTCTCGTTCGTTCATTCCTTAGCGTTTTTTGTTGAATCCAATCCAATCGAAGAAGAAGAAGATGGGAAAAAATATTGTGCCGGTGAAATCTGTTGTGTATGCTCTTTCTCCTTTCCAGCAGAAAATCATGAGCGGTTTGTGGAAGGATTTGCCAACCAAAATTCATCACAAGGTCTCCGAAAATTGGATCAGCGCTATTCTCTTGGTTACTCCCGTCGTCGGCACTTACTCGTATGCTATGCACTACGTGGAACAGGAGAAGCTGCACCACAGGTACTGAGCTCATGCAAGGATACATTGGATAATCTTCAAGTTTCATGTTCTTAATTTTGTTCAGCTAGTCAATAATGTGACATTTGTGAAATTAGCTATGGCCCTGTAGTTGTGATATTTTGAGCTTTAGCTGTTTCCACTATGAAGAATTTTATTGATAGCTTTCTTTGTTTTACATATCTACATGTAACAAAAGATAATCTATGTCTGTCAATAATCTTATGATGAGTGCCAAAATAAGTTTATATGTCAACCACTGGAGTGGTATGTGCTTTTATCAATTTAACAATCACTCTAATTACAAAACCAATTTTTGATCAGTTTTATTAAAT
TARGET=90,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3415
PRIMER_LEFT_PENALTY=0.328094
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=GTTCGTTCATTCCTTAGCGTTT
PRIMER_RIGHT_SEQUENCE=GCTGGAAAGGAGAAAGAGCATA
PRIMER_LEFT=46,22
PRIMER_RIGHT=159,22
PRIMER_LEFT_TM=59.672
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=0.3559
PRIMER_LEFT_1_PENALTY=0.287182
PRIMER_RIGHT_1_PENALTY=0.068749
PRIMER_LEFT_1_SEQUENCE=CATTCTCCTCCTCATTCTCGTT
PRIMER_RIGHT_1_SEQUENCE=GAATAGCGCTGATCCAATTTTC
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=240,22
PRIMER_LEFT_1_TM=59.713
PRIMER_RIGHT_1_TM=60.069
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=0.3968
PRIMER_LEFT_2_PENALTY=0.328094
PRIMER_RIGHT_2_PENALTY=0.068749
PRIMER_LEFT_2_SEQUENCE=GTTCGTTCATTCCTTAGCGTTT
PRIMER_RIGHT_2_SEQUENCE=GAATAGCGCTGATCCAATTTTC
PRIMER_LEFT_2=46,22
PRIMER_RIGHT_2=240,22
PRIMER_LEFT_2_TM=59.672
PRIMER_RIGHT_2_TM=60.069
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.4514
PRIMER_LEFT_3_PENALTY=0.328094
PRIMER_RIGHT_3_PENALTY=0.123278
PRIMER_LEFT_3_SEQUENCE=GTTCGTTCATTCCTTAGCGTTT
PRIMER_RIGHT_3_SEQUENCE=TTCTGCTGGAAAGGAGAAAGAG
PRIMER_LEFT_3=46,22
PRIMER_RIGHT_3=163,22
PRIMER_LEFT_3_TM=59.672
PRIMER_RIGHT_3_TM=60.123
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=0.4653
PRIMER_LEFT_4_PENALTY=0.287182
PRIMER_RIGHT_4_PENALTY=0.178157
PRIMER_LEFT_4_SEQUENCE=CATTCTCCTCCTCATTCTCGTT
PRIMER_RIGHT_4_SEQUENCE=CATAGCATACGAGTAAGTGCCG
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=281,22
PRIMER_LEFT_4_TM=59.713
PRIMER_RIGHT_4_TM=59.822
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=11.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_03974 Ps_contig_mira-and-tgicl-ass_franssen_31644
SEQUENCE=AATAAGTAAAAAAATNAAAGTAACTAAAATAAAATTACCGAATTAAAGAATAAATAAATAAACAAGATTTAACATTATTAGAACAAGATCTATCCAATCTTGTGATAAACCATTTTGTACAAAATTTATGTCTCTGAATTCATGAAATTGAAGTAATGATTGAGGGACCACTTTGAACTTGAACTATCTCTTTCTTTATGTCTGTCCATTAAAAGAATCATAAAATCCCAAACATTGATTCATCACACTACCTAGCTACCTGTCTTCTTTCTGATGCTGTTGTTTTTCTTGATGCCATTTTTGGATGTTGCTGATTTTTTGCTTAATTTGTATTTCATCATTCTTACAATTTTGAAAAACCAGAATGTACTTACAAGCTGCAACCCCAATCCCATTGCCTTTATGAGAAATGGATTATTGGAAGTTAATGTTATGTAACTGATACATGGTCCAATCATCATCCTAGCAAATGTGAATATTGAAGCAAATAGAATATCAGCAGTCAAGTTAAGGGCAGTGTCCTTGTAACCAAGTTCCTTTAGAAGTTCTCTCAAATGTAAAAATGGGCTTGACATTTCTGTTACCCAAACTGCCCCTACCATCTCTGACCCACACTTTTGATAACAGAGACCTGCTATGAGTCCAACAATGCTTACCAAATGATGGATACTGTTATCCAAATTGATTTTTTCGTCGAATAAACAACAAACTAGATCATAAATCAGATACGAAAGACTCACCGCAAGAACTTGCATCTGTCTGTGGGAAGAAGTTGCTGCCACAGGACAAATTGGACATTTCCAATCTTGAACCGATAAAGTTGCTAACGTTACCGCTAATATTGCATGAATTGTAGAAACAATTCTGTTGCAGAAATCAAATGAACGTTTTGGAAAGATTTTTCTTACTACAAGAAAAGTTGTCGCCCATGAAAAAACTCCAACAACGATTAACTTTTTAATGTACTCTTCCAACCCTTTATCATCTTCCAAACTTGAAAAAGAAAATAATAACCCTCGTAGATCGACTTCTCGTGGACCCACTTCAAACCCGACCCAAAAACCCGTAACCCGACCCATTGCTCGCGTTGATCGTAGATCCTCATCTTCGTTTAAGTCTCTTCCGAGAGGAAGAAGTCCTAGCCCTTCCGATCTCAGTCAAACTCGCCGTGTCTCCGTTGACCAAGCCGTGAAAGATTCGACCCATTTGAAATCCCGCAAAGATTCCAGCGCTAAATCAGCAAATAACCCCAGAAGCACTGAAAAGGTTAAAGCTTCACAA
TARGET=49,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03975 Ps_contig_mira-and-tgicl-ass_franssen_31647
SEQUENCE=TCTGCTGCTTACATGAATGGTGTTGATCGTGAAGGGCATTCTGTGTGTTACAACATCTATGGAGTTTTTGCTGGTGAAGAGATTTATCAAAAGAGTTTTGGGAGTGAAGAGAAGAGAAGAGAGTTTTTGAGATGGAGGTGTTATGTTACTGAGAAATGGATTCAGAAGCTGGATTTGAAACCAGGTGGTGTTTCTTCTTTGCTTCAAATCAACGACCTTAAGAATTGTCCCGGTCCTTCCAAGAAAGAGATAAGAATTGCTACAAATCAAATTGTTTCAATTTTGCAGGACAATTACCCTGAAATGGTGGCCAAAAATATTTTCATCAATGTTCCATTCTGGTATTATGCACTCAATGCACTTTTATCTCCATTCTTAACTCAAAGAACGAAGAGCAAATTCGTGGTGGCGCGTCCCGCCAAGGTGACCGAAACATTGATCAAGTACATTCCAATCGAGGAGATCCCAGTTAACTACGGCGGTTTCAAGAGGGAAAATGATTCCGAATTCTTCGGCCAAGACGCAACTGTTTCTGAACTGATTCTCAAGGCTGGATCGACCGCAACTATAGAGATACCTGCATTGGAAGCTGGAAACACTCTGTGCTGGGACATAGCTGTTTTGGGTTGGGAGGTAAGTTACAAAGAGGAATTTGTTCCCACTGATGAAGGCTCTTACACAGTCATTGTTTCGAAAGTGAAGAAGATAGGTTCACAAGAAGGAGCTATTAGGAACACATTCAAGAACAATGAAGCAGGAAAGGTTATTCTCACAGTTAACAACTCGTCGAACAAGAAGAAGAGGGTTATGTACCGTTACCAGATCAACAAGAACTCCCCATGAGAACTCAATTATTAAGTTCTTTTAGGTTTGTTCCATTGTTCTTTTGGTTTCATTTAGGTTTTGAAAGTAGGTGGTGGAATGTGTGAAGTTTTGGAGACTGGAGGAAAAAGAAGAGATTCTTTGATTTGATATTACATATTTTTTAAACTTGTTGACATTCATTTAGAGTTCATTCATTTTGTGTCAATGATATATACCTATCTATCTCATCTTATTTTGATGTAAAAAGTTTTTTCTTCATTTCATGAAAGACTTGATAAAATGAAAGTTTGCTTGAG
TARGET=106,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1659
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.128591
PRIMER_LEFT_SEQUENCE=GTGAAGGGCATTCTGTGTGTTA
PRIMER_RIGHT_SEQUENCE=GGGACAATTCTTAAGGTCGTTG
PRIMER_LEFT=28,22
PRIMER_RIGHT=230,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.871
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=0.1659
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.128591
PRIMER_LEFT_1_SEQUENCE=TGAAGGGCATTCTGTGTGTTAC
PRIMER_RIGHT_1_SEQUENCE=GGGACAATTCTTAAGGTCGTTG
PRIMER_LEFT_1=29,22
PRIMER_RIGHT_1=230,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.871
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.1677
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=GTGAAGGGCATTCTGTGTGTTA
PRIMER_RIGHT_2_SEQUENCE=GAAACACCACCTGGTTTCAAAT
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=193,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=0.1677
PRIMER_LEFT_3_PENALTY=0.037339
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=TGAAGGGCATTCTGTGTGTTAC
PRIMER_RIGHT_3_SEQUENCE=GAAACACCACCTGGTTTCAAAT
PRIMER_LEFT_3=29,22
PRIMER_RIGHT_3=193,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.1884
PRIMER_LEFT_4_PENALTY=0.037339
PRIMER_RIGHT_4_PENALTY=0.151106
PRIMER_LEFT_4_SEQUENCE=GTGAAGGGCATTCTGTGTGTTA
PRIMER_RIGHT_4_SEQUENCE=GAAGCAAAGAAGAAACACCACC
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=204,22
PRIMER_LEFT_4_TM=60.037
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_03976 Ps_contig_mira-and-tgicl-ass_franssen_31653
SEQUENCE=AACCCCTCCTTCGACCTTATCCTTCCTCAACGGCCTTATGTTGTTANNGAACTGCACAATTCCGTTGAGATCTATTTCAGCAATAAGAACTCCCAAAGCTTCACATTAGGGTTTTGTTTCGATTGAATTCGTTTGTGAAATTCGCATAAGGTTAAGATGTTTGGGCGAGGAGCACCGAAGAAAAGCGATAACACAAAATATTATGAGATTCTCGGCGTTTCGAAGACCGCTTCTCCTGATGATTTGAAGAAAGCTTACAAAAAAGCTGCCATCAAGAATCATCCTGATAAGGGAGGTGATCCTGAGAAGTTCAAAGAGCTTGCTCAAGCTTATGAGGTTTTGAGTGATCCCGAGAAACGTGAGATATATGATCAATACGGTGAAGATGCGCTTAAGGAAGGAATGGGTGGCGGCGGCGGTGGCCATGACCCATTTGATATCTTTTCGTCTTTCTTTGGTGGTGGTGGATTTCCAGGTGGTGGTAGTAGAGGCCGAAGGCAGAGACATGGGGAAGATGTTGTTCACCCCTTGAAGGTCTCTTTGGAGGATCTTTACCTTGGGACATCTAAGAAGCTTTCTCTCTCAAGAAATGTCTTGTGTTCCAAGTGCAATGGGAAAGGGTCTAAGTCTGGGGCTTCCATGACATGTTCTGGTTGTGATGGAACTGGTATGAAGATTTCTATGAGGCATCTGGGTGCCAACATGATTCAGCAAATGCAACATCCTTGCAATCAATGTAAGGGTACTGGAGAGACAATTACTGACAAAGATCGATGCCAACAGTGCAAGGGAGAGAAGGTTGTTCAACAGAAGAAAGTACTTGAAGTTCATGTTGAAAAGGGAATGCAGAACGGGCAGAAAATTACGTTCCCTGGTGAAGCTGATGAAGCGCCGGATACAGTTACTGGAGATATTGTATTTGTACTTCAACAGAAGGAGCATCCGAAGTTCAAAAGAAGTGGTGATGATCTTTTTGTAGAGCACACCTTGTCCCTCACTGAAGCCTTATGTGGTTTCCAGTTCGCCCTGACTCATTTGGATGCTCGGCAACTGTTGATTAAATCAAATCCCGGAGAAGTTGTCAAGCCTGATTCATATAAGGCAATTAATGATGAGGGCATGCCAATGTACCAGAGGCCATTCATGAAGGGCAAGCTTTACATTCACTTCACTGTGGAGTTTCCCGAGTCTCTGTCTCCTGATCAGGTCAAGGCTTTGGAGACAATTCTGCCAGCACGGCCTTTATCACAGTTGACAGAAATGGAGATAGATGAGTGTGAGGAGACAACACTTCATGATGTCAACATGGAGGAAGAGTCAAGGAGGAAGAAGCAAGCTCCGCAAGAGGCTTATGACGAGGATGATGAAATGCCTGGTGGTGCTCAGAGAGTCCAATGCGCTCAGCAGTAATGAGTTTGGTGGAATTCGTGGTTAATGTTATCCATATAGTAGTGATGGAATGATTTTTGATACAGTGTAGCATTTATTTCTTTATAAAGATACCCTATAACGCTTGATATATTATTATTTGCTTATTCTGGCAGCTAACTATTGTTCCCTCATATATCAAGCAATTTTAGTTTTCCTTTCCGTGTTTCTTTCTGTTGCTTATGATGTTCTTCAAATTTCTACTCATCGTTAACATTCTTTGGGTTTTGTTCGAAGGT
TARGET=456,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1848
PRIMER_LEFT_PENALTY=0.047267
PRIMER_RIGHT_PENALTY=0.137575
PRIMER_LEFT_SEQUENCE=GCCATGACCCATTTGATATCTT
PRIMER_RIGHT_SEQUENCE=ACAACATCTTCCCCATGTCTCT
PRIMER_LEFT=421,22
PRIMER_RIGHT=520,22
PRIMER_LEFT_TM=60.047
PRIMER_RIGHT_TM=59.862
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=0.2805
PRIMER_LEFT_1_PENALTY=0.047267
PRIMER_RIGHT_1_PENALTY=0.233224
PRIMER_LEFT_1_SEQUENCE=GCCATGACCCATTTGATATCTT
PRIMER_RIGHT_1_SEQUENCE=GAACAACATCTTCCCCATGTCT
PRIMER_LEFT_1=421,22
PRIMER_RIGHT_1=522,22
PRIMER_LEFT_1_TM=60.047
PRIMER_RIGHT_1_TM=60.233
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=0.2805
PRIMER_LEFT_2_PENALTY=0.047267
PRIMER_RIGHT_2_PENALTY=0.233224
PRIMER_LEFT_2_SEQUENCE=GCCATGACCCATTTGATATCTT
PRIMER_RIGHT_2_SEQUENCE=AACAACATCTTCCCCATGTCTC
PRIMER_LEFT_2=421,22
PRIMER_RIGHT_2=521,22
PRIMER_LEFT_2_TM=60.047
PRIMER_RIGHT_2_TM=60.233
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=101
PRIMER_PAIR_PENALTY_3=0.3301
PRIMER_LEFT_3_PENALTY=0.047267
PRIMER_RIGHT_3_PENALTY=0.282868
PRIMER_LEFT_3_SEQUENCE=GCCATGACCCATTTGATATCTT
PRIMER_RIGHT_3_SEQUENCE=TCATACCAGTTCCATCACAACC
PRIMER_LEFT_3=421,22
PRIMER_RIGHT_3=672,22
PRIMER_LEFT_3_TM=60.047
PRIMER_RIGHT_3_TM=59.717
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.3489
PRIMER_LEFT_4_PENALTY=0.211336
PRIMER_RIGHT_4_PENALTY=0.137575
PRIMER_LEFT_4_SEQUENCE=ATCATCCTGATAAGGGAGGTGA
PRIMER_RIGHT_4_SEQUENCE=ACAACATCTTCCCCATGTCTCT
PRIMER_LEFT_4=277,22
PRIMER_RIGHT_4=520,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=59.862
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=244
=
PRIMER_SEQUENCE_ID=SSR_03977 Ps_contig_mira-and-tgicl-ass_franssen_31657
SEQUENCE=TATAGGCAATCCANTACAAGATTNTGCATTATGAGGGAAGTATCAGAATAGTTCAATAATAAAGATTAAAGGTTTAATATGGACGATCTGCGCCTCAGAGTAGCTCATTACATGATCAAATCACTTTCAAGTATACAATAATGAAAAGGCAAAATATACCCCATGGTGACCACAAGAGCCATCACAACTAGATAGCAACCTATCAAGCAATTGAGTTGGTATATCAGATGGAAAACTACATCTCAAAATTCAATGTGGACTCAACACTGAGTTTGGGAATTTTCTTTTCCTCGGCCAAGTTAACGGATGCCACAACCTTGAAGCAACCAGTTTGTTTGGAATTCCTTGTCCACTCCAATCCTAACACCTTGCTTGATGTCTGATAAGAAGCTTTGAGAGAATCATCACCATAAACTTTTCCAGAAACTGCAAAGTCCCAAGAATTCTTAGCAACATCATAGGTAGGTTCAATTGTAGTCAAACCCTTATGATTATAGCTATACTTCAATTTACAGTTTCCAGATTCAACAGCGTAATTCGCTGAAACCTTGTTCGATGGGTCCCACACTAACGTTCCGTCCAAAATTGTTCTGTTATCTCCTTTGCTATGTATGTATGCCAAATTCAACGGTTTCTCTGCAACCCTAACGGTGTTCATGAATTGAAACCGAAAATCCTTTTTGGGGACGTTGTAGTCGACACTGAAGGAGCCGGGTTTCTCTACGGCGAGGACTAGGCCGGTTAAGCTGGGGCTGTTTTTGAAAGTGGCGTCGGTTATTGATGCTCGGAGTTTGACATCGCCGGCGTTGAAGGCGACGGTGGCGGCACCGCTGCTGTTATGGTCAAGGTCGTATTTGCCCTTCAAAGCGGCCTTCATTGTTAACAACGGAGTGGAGAAAGGGATGAAAATGCGAATATTTTTTTTTGGTATTTTTTTGTTGAAAATCCCGCC
TARGET=607,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3347
PRIMER_LEFT_PENALTY=0.195574
PRIMER_RIGHT_PENALTY=0.139129
PRIMER_LEFT_SEQUENCE=ACACTAACGTTCCGTCCAAAAT
PRIMER_RIGHT_SEQUENCE=TCAATTCATGAACACCGTTAGG
PRIMER_LEFT=564,22
PRIMER_RIGHT=664,22
PRIMER_LEFT_TM=59.804
PRIMER_RIGHT_TM=59.861
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=0.4108
PRIMER_LEFT_1_PENALTY=0.195574
PRIMER_RIGHT_1_PENALTY=0.215254
PRIMER_LEFT_1_SEQUENCE=ACACTAACGTTCCGTCCAAAAT
PRIMER_RIGHT_1_SEQUENCE=TTCAGTGTCGACTACAACGTCC
PRIMER_LEFT_1=564,22
PRIMER_RIGHT_1=705,22
PRIMER_LEFT_1_TM=59.804
PRIMER_RIGHT_1_TM=60.215
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=142
PRIMER_PAIR_PENALTY_2=0.4139
PRIMER_LEFT_2_PENALTY=0.274768
PRIMER_RIGHT_2_PENALTY=0.139129
PRIMER_LEFT_2_SEQUENCE=AGAAACTGCAAAGTCCCAAGAA
PRIMER_RIGHT_2_SEQUENCE=TCAATTCATGAACACCGTTAGG
PRIMER_LEFT_2=421,22
PRIMER_RIGHT_2=664,22
PRIMER_LEFT_2_TM=60.275
PRIMER_RIGHT_2_TM=59.861
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.4773
PRIMER_LEFT_3_PENALTY=0.261998
PRIMER_RIGHT_3_PENALTY=0.215254
PRIMER_LEFT_3_SEQUENCE=GTTCCGTCCAAAATTGTTCTGT
PRIMER_RIGHT_3_SEQUENCE=TTCAGTGTCGACTACAACGTCC
PRIMER_LEFT_3=572,22
PRIMER_RIGHT_3=705,22
PRIMER_LEFT_3_TM=60.262
PRIMER_RIGHT_3_TM=60.215
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=134
PRIMER_PAIR_PENALTY_4=0.4900
PRIMER_LEFT_4_PENALTY=0.274768
PRIMER_RIGHT_4_PENALTY=0.215254
PRIMER_LEFT_4_SEQUENCE=AGAAACTGCAAAGTCCCAAGAA
PRIMER_RIGHT_4_SEQUENCE=TTCAGTGTCGACTACAACGTCC
PRIMER_LEFT_4=421,22
PRIMER_RIGHT_4=705,22
PRIMER_LEFT_4_TM=60.275
PRIMER_RIGHT_4_TM=60.215
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_03978 Ps_contig_mira-and-tgicl-ass_franssen_31660
SEQUENCE=GATTGAACGGGAGAGAATATGTCAAAAATTTAGGATTTGCAATGGAAAACCCTAAATACTACTTTTTGTTGAATGTTAGGGTTTGACTGGTTTGTGATTGCGATTTGCGAGGACGCGTCACTAATCAGGAATCCAAATTCCAATTTCAAATCTATCAACGAAGAGGAACAGAGTCTCTGTCGCCGCTTTATCGGGTTTTATCTTCATGTACTAATACCATGAAGAGGACATTGCCGTGGAGTGATGATGATGAGGGTGATGACTCATCTAATGATGAGTCGTCTTCTTTACATTCAGGTTCTGAAGGTGATAATGAAACTGGAGGAAAAAAATCCAAAGGTAAATCTACGAAGAAGAAAAGTGCTCCAATTGACTTTGAAGCTCTCAAGCGATACGGGTATAAAGGCGGGCCTTCAGTCTTAAAGGTACCTCCACCCAAAGAAGGTGACACAAAGCAAGATTGGTCTTGGTCCAGTGGAAAAGAAAAGCGCGTCGAGAAGGAGGTTGAAGAAACATATGAAGAACGGAAGAAAACAAGAGAGGCTCTTTCCATAGGAGAGCAGCTGCCAACTGTTCTGACTAGAAATGAAAAGAAGAGTCTTTCCTTTTCTCAGAAGGAAAAGAGGAAAAGGGAACTTGGTCAAGCAAGCAGAGGGAAAAATTATGTTGAAGAAGAGAAAAGGCAGTTGAGGGAGAATGGAGTCTATTCAGGTTTTGATGCTTGATATGCTGAGGCTAGAATCTACTGCATCTTGTATAAAGGTTACTATGATACTATCCAGGGCCATTTTGTACTAAAATGATTTGGTTTAGGTGATGTCTCTACTATAATCTCATGTTAAGTCGAAACCATTTTAGATATTAAATGACTGAGGCTGGAATCTTTTATGGATAGCCAAAATTGAACGTCACATAACATATTATCGATTAATTTTGGTTGTTCAAC
TARGET=242,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3347
PRIMER_LEFT_PENALTY=0.226971
PRIMER_RIGHT_PENALTY=0.107763
PRIMER_LEFT_SEQUENCE=AGGGTTTGACTGGTTTGTGATT
PRIMER_RIGHT_SEQUENCE=ATTGGAGCACTTTTCTTCTTCG
PRIMER_LEFT=77,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=59.773
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.3498
PRIMER_LEFT_1_PENALTY=0.215225
PRIMER_RIGHT_1_PENALTY=0.134571
PRIMER_LEFT_1_SEQUENCE=ATACCATGAAGAGGACATTGCC
PRIMER_RIGHT_1_SEQUENCE=TTGGGTGGAGGTACCTTTAAGA
PRIMER_LEFT_1=213,22
PRIMER_RIGHT_1=438,22
PRIMER_LEFT_1_TM=60.215
PRIMER_RIGHT_1_TM=59.865
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.3512
PRIMER_LEFT_2_PENALTY=0.215225
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=ATACCATGAAGAGGACATTGCC
PRIMER_RIGHT_2_SEQUENCE=TTTCTTCAACCTCCTTCTCGAC
PRIMER_LEFT_2=213,22
PRIMER_RIGHT_2=512,22
PRIMER_LEFT_2_TM=60.215
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.4351
PRIMER_LEFT_3_PENALTY=0.215225
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=ATACCATGAAGAGGACATTGCC
PRIMER_RIGHT_3_SEQUENCE=AGACCAATCTTGCTTTGTGTCA
PRIMER_LEFT_3=213,22
PRIMER_RIGHT_3=466,22
PRIMER_LEFT_3_TM=60.215
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.5331
PRIMER_LEFT_4_PENALTY=0.215225
PRIMER_RIGHT_4_PENALTY=0.317850
PRIMER_LEFT_4_SEQUENCE=ATACCATGAAGAGGACATTGCC
PRIMER_RIGHT_4_SEQUENCE=TTGCTTTGTGTCACCTTCTTTG
PRIMER_LEFT_4=213,22
PRIMER_RIGHT_4=457,22
PRIMER_LEFT_4_TM=60.215
PRIMER_RIGHT_4_TM=60.318
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_03979 Ps_contig_mira-and-tgicl-ass_franssen_31662
SEQUENCE=CCGGGGGACCACACACACAAAAAAAGAACCATGTCTAGCTTCACTCATGCCACCACACTACTCCATGCTCACATCAAAACCAAACACAAAATCACTTCAAACAACAACATTAACAACAACCACAACAAAAACAACCTTTCATCTTCTAAACAAAATGCAACCAATAGAAGAAATTTAATTTCAACATTTCTAGCTACATCAATGGTTGTTGGCACAGCACCATCAGCATCAGCACAGAATTGGGGCACTCGTTCTTTCCTGAGAGAACATTTTTTTCAGCCAGGGCTTTCCCCTGAAGATGCGGTTTTAAGGATAAAACAAACAGCTGAAGGGTTACATAATCTTAGGGAAATGTTGGAAACTTTGTCTTGGAGGTATGTTATGTTTTATATAAGATTGAAACAGAACTATCTTGAACAGGATTTGAAAAATGCTATGACTACTTTGCCGGAAAATCGTCGCAAGGAGTATGTCAAAACTGCTAACGAATTGGTTGATCACTTGACTGAGATGGATCGTTATGTTCGATCGCCAAAGGTATATGAGTCGTATTTATACTACGAGAAGACATTGAAATCAATAGATGAACTTGTAGCTATATTAGCATAGTTCAAAGGTGAAGCTGCAGGGTATTGGTATTTTGAATTATAATTGTATAAACACATAATCTTGACATTATTTTTCTTCTGAATTTTGGATTTTTTGATTGAACATATATAGAAGGTTTACCTGGATGTAAAACTTTTAAGTATATTATTATGTACATCTTTGTCATTAGAATATGCAACTTCAAGATTTTTTATTTATCGARAGGTTTGGTTTATAAAG
TARGET=10,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03980 Ps_contig_mira-and-tgicl-ass_franssen_31666
SEQUENCE=TCGGGGTAAACCGAAAAACCTAAACTTTTCGAAGAAGAAGAATTCATCTATCTGGTATTAACCCTAAAGAAGATTCCTACAAATGGCCGTGTTAACCCTCACAAGATTCCCATCCCTCACTCCCTTCTCTCTCCGTCATCGGAGCAATGTCTCATCATCGACGACAGAGCCAACAAGCCTGCTCGTCTCACCAAAGATGAAGCTCAGAAGAAGATTCAATCGGAGTCGATTCCTATCGCGAAGGTTCTCAAGCTCTCGAAGCTTACTACGGATTATCGTCCGTTTGAGGCGAAGCGTAAGCTGTGTAATTCGTATGATCTGTTTTTTGCTGATAAAACTATTGTGCCGTTGCTGCCGCGTTTGCTTGGGAAGCAGTTTTTTAAGAAGAGGAAGCTTCCGGTGCAGGTTGATTTGAAGAAGAAGAATTGGAAGGAGCAGATTGAGAGAGCGTGTTCTTCTGCGCTATTGTTTTTGAGGACTGGGACCTGTAGTGTTCTGAAAGTGGCGAAGTTGAGTATGGAGAGGGATGAGATTGTTGAGAATGTGGTTGCTGCTATGGAGGGGGTTGTGGAGGTTTTTCCGAAAAAATGGGGGGTTGTTAGGTCTTTTCATGTTAAGTTGTTGGAGTCTGTTGCGTTGCCGGTTTATCAGGCTGTTCCTGATGTTAGGTTGAAGATTGAGGGGGTTAGGGATTTGGAGGCTGCTTTGGAGAAGGATGAGAAGAAGGTTCGTGATGGGAAAGACGGGAAGAAGAGTAAGAAGGGGAGGATTCATGAAATTAAGTATATGGATGATAGAATGAGTGAGGATGGTGATGTTCGTAGTGATGTCGATGTTGATGATATTGATGATGGTGAAGTTGGTGGTGGTGATAATGTAGATGATGATCACGAAAAGGGTGAAATTGAGAGTGGAGCTCTGGGGAGTAAGAAGAGGAAGAAAGGAGTTAAAGGGAAGAAAGAGGCTTTGAGTGAGTCGGGCAGTGTGAAATCGTTGAAGGGTTCTGCTAAGAGGAAAAAGAAAGATGTGTTGGTGACTCTGAATGCTGCGGAAGAAGAAGGTTCCATCAAGAAGGGTAGTGAAAAGAGAAAGAAATTGTCTGCGAAAGACGAGGGTTCAGACAAGAAGAAGAAAAAAGGGTCGGTTGAGGATAAAGCTGCTACTGAAGACTTGATCAAGAAGAGTGAGGATAAGGTATCAATTGAGGATGCGGGTTCGGACAAGAAGAAGAAAAGAGGGTTGGCTGTGAATGCTGCCACCGAAGATTTGATCAAGAAGAGCAAGGATAAATTATTCACCGAGGATGCTGGTTCTGACAAGAAGAAGACAAGGAGAGCCGATGTGAAGGTAAAGGGTAGGAAAAGTAAGAATGCTGTATGAACCTACTCTTGATGTTGGAACAATTTTGTTGTTACAATTTATTGCTTGCTGTGGCTCTGTGCAATCATGTGTTGTTTTGCCTATGAGTTCTCTGGCAGATTTTGTTTTCTGAGAATTTATGGTATTTAATGTGTTTTGTTTTTGGTTATAATGTTG
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4248
PRIMER_LEFT_PENALTY=0.417844
PRIMER_RIGHT_PENALTY=0.006945
PRIMER_LEFT_SEQUENCE=GGGGTAAACCGAAAAACCTAAA
PRIMER_RIGHT_SEQUENCE=TTCTGAGCTTCATCTTTGGTGA
PRIMER_LEFT=2,22
PRIMER_RIGHT=208,22
PRIMER_LEFT_TM=60.418
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.4277
PRIMER_LEFT_1_PENALTY=0.417844
PRIMER_RIGHT_1_PENALTY=0.009871
PRIMER_LEFT_1_SEQUENCE=GGGGTAAACCGAAAAACCTAAA
PRIMER_RIGHT_1_SEQUENCE=CGGACGATAATCCGTAGTAAGC
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=281,22
PRIMER_LEFT_1_TM=60.418
PRIMER_RIGHT_1_TM=60.010
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.5594
PRIMER_LEFT_2_PENALTY=0.417844
PRIMER_RIGHT_2_PENALTY=0.141511
PRIMER_LEFT_2_SEQUENCE=GGGGTAAACCGAAAAACCTAAA
PRIMER_RIGHT_2_SEQUENCE=CTGTCGTCGATGATGAGACATT
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=166,22
PRIMER_LEFT_2_TM=60.418
PRIMER_RIGHT_2_TM=60.142
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.6048
PRIMER_LEFT_3_PENALTY=0.417844
PRIMER_RIGHT_3_PENALTY=0.186949
PRIMER_LEFT_3_SEQUENCE=GGGGTAAACCGAAAAACCTAAA
PRIMER_RIGHT_3_SEQUENCE=GATGGGAATCTTGTGAGGGTTA
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=113,22
PRIMER_LEFT_3_TM=60.418
PRIMER_RIGHT_3_TM=60.187
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=112
PRIMER_PAIR_PENALTY_4=0.6256
PRIMER_LEFT_4_PENALTY=0.417844
PRIMER_RIGHT_4_PENALTY=0.207756
PRIMER_LEFT_4_SEQUENCE=GGGGTAAACCGAAAAACCTAAA
PRIMER_RIGHT_4_SEQUENCE=TCGACTCCGATTGAATCTTCTT
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=228,22
PRIMER_LEFT_4_TM=60.418
PRIMER_RIGHT_4_TM=60.208
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_03981 Ps_contig_mira-and-tgicl-ass_franssen_31666
SEQUENCE=TCGGGGTAAACCGAAAAACCTAAACTTTTCGAAGAAGAAGAATTCATCTATCTGGTATTAACCCTAAAGAAGATTCCTACAAATGGCCGTGTTAACCCTCACAAGATTCCCATCCCTCACTCCCTTCTCTCTCCGTCATCGGAGCAATGTCTCATCATCGACGACAGAGCCAACAAGCCTGCTCGTCTCACCAAAGATGAAGCTCAGAAGAAGATTCAATCGGAGTCGATTCCTATCGCGAAGGTTCTCAAGCTCTCGAAGCTTACTACGGATTATCGTCCGTTTGAGGCGAAGCGTAAGCTGTGTAATTCGTATGATCTGTTTTTTGCTGATAAAACTATTGTGCCGTTGCTGCCGCGTTTGCTTGGGAAGCAGTTTTTTAAGAAGAGGAAGCTTCCGGTGCAGGTTGATTTGAAGAAGAAGAATTGGAAGGAGCAGATTGAGAGAGCGTGTTCTTCTGCGCTATTGTTTTTGAGGACTGGGACCTGTAGTGTTCTGAAAGTGGCGAAGTTGAGTATGGAGAGGGATGAGATTGTTGAGAATGTGGTTGCTGCTATGGAGGGGGTTGTGGAGGTTTTTCCGAAAAAATGGGGGGTTGTTAGGTCTTTTCATGTTAAGTTGTTGGAGTCTGTTGCGTTGCCGGTTTATCAGGCTGTTCCTGATGTTAGGTTGAAGATTGAGGGGGTTAGGGATTTGGAGGCTGCTTTGGAGAAGGATGAGAAGAAGGTTCGTGATGGGAAAGACGGGAAGAAGAGTAAGAAGGGGAGGATTCATGAAATTAAGTATATGGATGATAGAATGAGTGAGGATGGTGATGTTCGTAGTGATGTCGATGTTGATGATATTGATGATGGTGAAGTTGGTGGTGGTGATAATGTAGATGATGATCACGAAAAGGGTGAAATTGAGAGTGGAGCTCTGGGGAGTAAGAAGAGGAAGAAAGGAGTTAAAGGGAAGAAAGAGGCTTTGAGTGAGTCGGGCAGTGTGAAATCGTTGAAGGGTTCTGCTAAGAGGAAAAAGAAAGATGTGTTGGTGACTCTGAATGCTGCGGAAGAAGAAGGTTCCATCAAGAAGGGTAGTGAAAAGAGAAAGAAATTGTCTGCGAAAGACGAGGGTTCAGACAAGAAGAAGAAAAAAGGGTCGGTTGAGGATAAAGCTGCTACTGAAGACTTGATCAAGAAGAGTGAGGATAAGGTATCAATTGAGGATGCGGGTTCGGACAAGAAGAAGAAAAGAGGGTTGGCTGTGAATGCTGCCACCGAAGATTTGATCAAGAAGAGCAAGGATAAATTATTCACCGAGGATGCTGGTTCTGACAAGAAGAAGACAAGGAGAGCCGATGTGAAGGTAAAGGGTAGGAAAAGTAAGAATGCTGTATGAACCTACTCTTGATGTTGGAACAATTTTGTTGTTACAATTTATTGCTTGCTGTGGCTCTGTGCAATCATGTGTTGTTTTGCCTATGAGTTCTCTGGCAGATTTTGTTTTCTGAGAATTTATGGTATTTAATGTGTTTTGTTTTTGGTTATAATGTTG
TARGET=414,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0077
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=TCACCAAAGATGAAGCTCAGAA
PRIMER_RIGHT_SEQUENCE=CCCAGTCCTCAAAAACAATAGC
PRIMER_LEFT=187,22
PRIMER_RIGHT=482,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=0.1507
PRIMER_LEFT_1_PENALTY=0.149938
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=TGCTGATAAAACTATTGTGCCG
PRIMER_RIGHT_1_SEQUENCE=CCCAGTCCTCAAAAACAATAGC
PRIMER_LEFT_1=326,22
PRIMER_RIGHT_1=482,22
PRIMER_LEFT_1_TM=60.150
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.7000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=0.1754
PRIMER_LEFT_2_PENALTY=0.174657
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=AGCTCTCGAAGCTTACTACGGA
PRIMER_RIGHT_2_SEQUENCE=CCCAGTCCTCAAAAACAATAGC
PRIMER_LEFT_2=250,22
PRIMER_RIGHT_2=482,22
PRIMER_LEFT_2_TM=59.825
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.1767
PRIMER_LEFT_3_PENALTY=0.149938
PRIMER_RIGHT_3_PENALTY=0.026744
PRIMER_LEFT_3_SEQUENCE=TGCTGATAAAACTATTGTGCCG
PRIMER_RIGHT_3_SEQUENCE=CGCCACTTTCAGAACACTACAG
PRIMER_LEFT_3=326,22
PRIMER_RIGHT_3=506,22
PRIMER_LEFT_3_TM=60.150
PRIMER_RIGHT_3_TM=59.973
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.1811
PRIMER_LEFT_4_PENALTY=0.149938
PRIMER_RIGHT_4_PENALTY=0.031122
PRIMER_LEFT_4_SEQUENCE=TGCTGATAAAACTATTGTGCCG
PRIMER_RIGHT_4_SEQUENCE=ACTACAGGTCCCAGTCCTCAAA
PRIMER_LEFT_4=326,22
PRIMER_RIGHT_4=491,22
PRIMER_LEFT_4_TM=60.150
PRIMER_RIGHT_4_TM=60.031
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.7000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_03982 Ps_contig_mira-and-tgicl-ass_franssen_31671
SEQUENCE=ATCAATTTATACTCTTGACCCTCTCTATCTCTAATACTTCAACTTACTTTCTTTTCTTGAGTCATTTGCGGAGAAGTTCTAAACATGAGTAGGCCAGGAGATTGGAACTGCAGGTCATGCCAACACATGAATTTCCAAAGGCGCGAATCGTGTCAAAGATGTGGCGATTCGAAATACGGAGATCGAGTTGATTATGGATCAGTGTTTGGAGGAATTAGAGGAGGATCTTCATTTGGATTAAGTGGTTCTGATGTTCGTCTAGGTGATTGGTATTGCGGTGCTGGTAATTGTGGTGCACACAATTTTGCTAGTCGTTTGAGTTGCTTCAAATGTGGTGCTTTCAAAGATGAATTAGCTGGTGGAGGATACAACAATAGTGCTGATATTTTGCTTTCAAGAGGCTTTGGTGGAAGTACTAGACCTGGATGGAAATCCGGAGATTGGATTTGTAACAGATTAGGATGCAATGAGCACAACTTTGCAAGCAGAATGGAATGTTTCAAATGCAGTGCACCAAGAGACACCTACTAGAGAAATGAGATTGTTGAAGAAAAAGAAAGACTAGAGATGGTGCAAGATTTCTTCATTAGTTACTATGAGATGTTAAGATTTATGATTATGATGATGATGATGATGATAGTTGAAAAGTGGTTTGAAATGACGTGTTATAAGAGAAACCACATTTCTTAGGATCTTCTTTAGATTTCCCTTTAAAAATTATAACAATGTTGTTTTGGTGTTTTAAACTATTTTTATTTTCTTTGTATTCTTTGTTGTTAGAGAGTTCTATTTATTTGTATGTGCGTAGGTTTTGTTGAATGAAGATGAATAT
TARGET=619,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.0723
PRIMER_LEFT_PENALTY=3.033117
PRIMER_RIGHT_PENALTY=0.039178
PRIMER_LEFT_SEQUENCE=AAGAAAGACTAGAGATGGTGCAAGA
PRIMER_RIGHT_SEQUENCE=TCATTCAACAAAACCTACGCAC
PRIMER_LEFT=553,25
PRIMER_RIGHT=821,22
PRIMER_LEFT_TM=59.967
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=269
PRIMER_PAIR_PENALTY_1=3.2756
PRIMER_LEFT_1_PENALTY=3.236447
PRIMER_RIGHT_1_PENALTY=0.039178
PRIMER_LEFT_1_SEQUENCE=GAAAGACTAGAGATGGTGCAAGA
PRIMER_RIGHT_1_SEQUENCE=TCATTCAACAAAACCTACGCAC
PRIMER_LEFT_1=555,23
PRIMER_RIGHT_1=821,22
PRIMER_LEFT_1_TM=57.764
PRIMER_RIGHT_1_TM=60.039
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=3.2984
PRIMER_LEFT_2_PENALTY=3.259178
PRIMER_RIGHT_2_PENALTY=0.039178
PRIMER_LEFT_2_SEQUENCE=AGAAAGACTAGAGATGGTGCAAGA
PRIMER_RIGHT_2_SEQUENCE=TCATTCAACAAAACCTACGCAC
PRIMER_LEFT_2=554,24
PRIMER_RIGHT_2=821,22
PRIMER_LEFT_2_TM=58.741
PRIMER_RIGHT_2_TM=60.039
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=3.5098
PRIMER_LEFT_3_PENALTY=3.470597
PRIMER_RIGHT_3_PENALTY=0.039178
PRIMER_LEFT_3_SEQUENCE=AAAGAAAGACTAGAGATGGTGCAAG
PRIMER_RIGHT_3_SEQUENCE=TCATTCAACAAAACCTACGCAC
PRIMER_LEFT_3=552,25
PRIMER_RIGHT_3=821,22
PRIMER_LEFT_3_TM=59.529
PRIMER_RIGHT_3_TM=60.039
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=3.7310
PRIMER_LEFT_4_PENALTY=3.691805
PRIMER_RIGHT_4_PENALTY=0.039178
PRIMER_LEFT_4_SEQUENCE=AAGAAAGACTAGAGATGGTGCAAG
PRIMER_RIGHT_4_SEQUENCE=TCATTCAACAAAACCTACGCAC
PRIMER_LEFT_4=553,24
PRIMER_RIGHT_4=821,22
PRIMER_LEFT_4_TM=58.308
PRIMER_RIGHT_4_TM=60.039
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_03983 Ps_contig_mira-and-tgicl-ass_franssen_31674
SEQUENCE=TGGGCTGAGGGATAGGGAGTGGGGAGGAGGAAGGTGATGAGGAGGAGGAAGAGGGTGGTCAGATTGCTGCGGAGCAGTTTGAGGATAATTGGGCTTCTGTTGTGGATGATGAAGCGGGTGAAAGGTTGCTGGGGAGAAACAGAAATGATGGTGAGGGTTTGGACAACAACAAGACGAAGAAGAAAGGGAAGAAAGCTGGGTATTTCAGTGATGAGAGTGATCATGATGATGATGATTGATCATGACTATGTAGCATTGACACTTTAGATTGAAGGCACTTGTGGTATTGAATCAATATGAAACCGGTTTGTCCTTTTGTTAAAATTCCTCGGCCGGACGATAGTAATGCTTCTAAAAAACCGAGTGAAAACTCAACTAAATATCATGTAGAACATGAAAGTAAGGTGAATAAAGAGGTTAATGACTCGGCCAGCGTTTCACCGAAGTGTCCAATGGGATATGACTCTCAGACGTTTAAGCTTGGTCCTCTTAGCTGTATGGTATGCCAAGCGCTGCTGTTCGACACCAGTAAATGTGTGCCTTGTTTGCATGTTTTCTGCAAAGCATGTATATCGCGCTTTGAGGACTGTCCGTTATGTGGAGCTGATATTGTGAAGATTGAGCCCGATGACAATCTTCAAGGTGTAGTCGATCGCTTTATTGAAGGTCATGCTAGGATCAAAAGGTCTGTTAATTTAGACAAAGGCGAAGAAGCAACAGAAAATAATAAGCCGGTTATATACGAAGATGTGTCTTTGGAAAGAGGTTCTTTCTTAGTACAACAGGCCATGAGGGCATTCCGCGCTCAGAATTTAGAAAGTGCCAAATCAAGACTCAGCCTATGTGCAGAAGACATTCGTAGTCAGATAGAAAAAGTTGGTAACACTTCAGAGTTATGTTCACAACTTGGAGCAGTCTTGGGTATGCTCGGCGACTGCTGCCGAGCAATGGGTGATAGCAGTTCTGCAGTAACTTATTTTGAAGAAAGTGTTGAATTTCTGTCAAAGTTGCCAAAAGATGATTTGGAGATTACACATACACTTTCCGTTTCACTTAATAAAATTGGTGATCTTAAATATCATGGGGGAGACCTCCAAGCTGCGAGATCATACTATTTTCAATCCTTAAATGTTCGCCGTGATGTTGTCAAGAACAATTCAAATGTTTCATCTCAGGTCTTAGATGTTGCTGTTTCTCTCGCAAAAGTAGCGGATGTTGACAAAAGTCTAGGAGATGAAAAGTCGGCTAGTGACGGATTTCAAGAAGCTATAAACTTGCTGGAATCACTTACTTTAAAATCTGAAGCCAGTGGGCTTGAGCAACGGCGGCTATCAGTTCTAGACTTCCTTAGAAGCCAACTGACAGATGATGATAAACAAGAACAAGTCGAACAAACAGTTTGAGGTCAGGTAGATGCTTAGCGAGCAAAAAGGAAACATCAATTCAGATGCAATAAATGTTTCTTTATATGATTGGATACGAATGTAAAATAAGCTCGGGTCTGCAGAGGCGTTAAAATAGAGAAAGGAAATCTTCATTTTGTTTCGAGTGATGAGGATCACTGTCATTTTGGGGACTGCAGTACTTGTCTTTGAGATTCTACAAAATTCCCTATGTGTTTCTTATACTACACCACTGTAGTTTTTGTATCCACCTCTTTAGTTGAAGCTTGGATCCTCTCCCGTTACGATTCTTCNGATGACCAGTACCTTATTTGNGCACAAGGGCTACCGTG
TARGET=223,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1614
PRIMER_LEFT_PENALTY=0.028588
PRIMER_RIGHT_PENALTY=0.132857
PRIMER_LEFT_SEQUENCE=AGGGAAGAAAGCTGGGTATTTC
PRIMER_RIGHT_SEQUENCE=TTGATTCAATACCACAAGTGCC
PRIMER_LEFT=184,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=59.867
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=111
PRIMER_PAIR_PENALTY_1=0.1614
PRIMER_LEFT_1_PENALTY=0.028588
PRIMER_RIGHT_1_PENALTY=0.132857
PRIMER_LEFT_1_SEQUENCE=GAAAGGGAAGAAAGCTGGGTAT
PRIMER_RIGHT_1_SEQUENCE=TTGATTCAATACCACAAGTGCC
PRIMER_LEFT_1=181,22
PRIMER_RIGHT_1=294,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.8000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=0.1670
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.132857
PRIMER_LEFT_2_SEQUENCE=GAGCAGTTTGAGGATAATTGGG
PRIMER_RIGHT_2_SEQUENCE=TTGATTCAATACCACAAGTGCC
PRIMER_LEFT_2=71,22
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.1670
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.132857
PRIMER_LEFT_3_SEQUENCE=GAGGATAATTGGGCTTCTGTTG
PRIMER_RIGHT_3_SEQUENCE=TTGATTCAATACCACAAGTGCC
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.867
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=0.1670
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_LEFT_4_SEQUENCE=TGAGGATAATTGGGCTTCTGTT
PRIMER_RIGHT_4_SEQUENCE=TTGATTCAATACCACAAGTGCC
PRIMER_LEFT_4=79,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_03984 Ps_contig_mira-and-tgicl-ass_franssen_31678
SEQUENCE=TCGGGRTACATACACTGCAAAAATATTCTTCTTCTTCTTCTTGTTCCGAAAGCAAAGGAAACAAACAGCATCATGTATCTTATCTTCTTTCTTTAAAAATAAATGCAAACTCTAAAATCAGAGAATTTCAAGGGTTTACAAATATTCTGTCTTCCCATTCTACCCTTGATTTTTCTCTAGATTTTCAGTTTCTCCCTTTTATCATAAAACGCACACGAACCCACGCAAATCGTCATTTCATATTCACAATGCTGTGTCATTTGGTTCCGTTGCCGCGGAAGAAAAAACCCGGTTCGGTTCCGGTTTACCTCAACGTTTACGATCTAACCCCAATGAATGGTTATGCTTATTGGTTCGGTCTCGGAGTTTATCATTCTGGTGTTAAAGTTCATGGTGTTGAATATGGATTTGGAGCACATGAACGTGAAACTACTGGGATTTTTGAAGTGGAGCCTGGACACTGTCCAGGATTCACCTTTAGGAAATCAGTTTATATTGGATCGACGGATCTGGGACCGAACGAGGTTCGGGAATTTATGGAAAAGCTATCTCAAGAGTATGCTGGAAATAGCTATCATCTAATACAAAAGAATTGCAACCATTTCTGTAATGATGTTTGTGTGAGGTTAACAGGAAAGTCTATTCCTCGGTGGGTTAACAGACTTGCTCGACTTGGTTTCTTCTGCAACTGCGTTCTTCCAACCAGCCTAAATGAAACAAAGGTTGGACAAGTTTCATTAGACAAGATTCATGAAGGAGAAAATAAGAAAATGAGAAGTCAATCCCGCAGGCAGGAGGCTTCTTCCAATCCTTCGTTACCTGCTAGCCAAAGACATTGTGGTATCCCATCTTCGTCTGTTATTAATGCTTCTTCATCATCAACTGTAGCAGTAAAATAAAGCTTTGGCATGAGTCAGAGTCTGAAACATAGGTTGTTAGAATCATCTATTGTATGAAAGATGGGTAGTAAAGCAACTTCTCATCTGCTCCGTCTGTCTGCTCTTCTGTATATCTTCAAATTGTGTTGATAATGGCGAAAATGAAATGACTGCTTTGGCTCTGTTTACTAAACGGATCTTTTTTAAAGATACAAGCTTATAAGGTCTGGACAAACAGTGCCTTAGTCTTTGTTCTCTTTAATTGTGTGCTCTCTTCATTAAACAAAGAAGCTTCTTTATTATTTTTAGATTTTTGGAGTAATCTTTTCTGGATCTTGCCTCATCTGTATTAGTTCTGTAGAATTTAGTGCTGTATTCATTGTAATTGCTCAAGTGTGAGATAATGCAAAGCTGGAAATATAAAACAGACAGTGAGTTGAAGGCAGTAGAGTTAAATTAAGCTCAAATTAGTGTTGAATTTCAT
TARGET=26,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03985 Ps_contig_mira-and-tgicl-ass_franssen_31684
SEQUENCE=AGTACGCGGGGAGAGTTTATTAAAAAAATTGAGTGAAAGGACTAAAGTTGGAGAGTTATTGAATGAATTGGCATGGTAATAGCAATATGTTATTTTATTGTAAGATGAAAGTTGAGCCCTAAAAGGAGAAGATAGATGATGATGATGATGATGCGGTGTGGAAATGGCGCAAGTGGAGTGCTATTCATGCCATCATGCTTTTGCTCCTGTTGGAAATGGAAACTCTATCCTCCTCCTCCTACAACGGAATTGGTTTCGCAGAAACGCAATCGGCGTCGGAGTGTGAAGGTGTTTTGTCAAAAAAGGGAAAATGTTAATTCTTCTTATGACTTCGATCCCGAGCTACGCTCCGCTCTTGAACTTGCTACTGACTCTGAGTTATGTGAAATTCAAACCATCCTTTTCGGCCCAAGCTACTTGAGTCCTTTGCTCAAATCCATCACATTAACAACTGCACCAACAACTACCCTCGACGAACATGACATTCAACTGCGCCAACAATTTATAGCCACACTCGAGTCACGCTTTTTCTACTTAGCAGCTGATGCACGCTCAACATTAAGGGGTTGGAGACCGACTTATAGAAATGTCTTGCTTCACCTAAGGAATAAATTCAATATTCCTTGCTCAACCAAGCTCTCTACTCACGACCTTGAACTTGAAATATTCCTCCATCTTTTACACTATAACTCTAATCAAGAACCAGGAAACCATACAGCCTTATTGGATGTATCCACCCAGTCTGATGCTCAGGGAACTCTACAGCTTGGACTCAATCAATGGAAAATGCACTCAAAAGTCGGAACACAAGACTTTCAATCTCTTCTTTTCAAGGGAGGTGGTATACTTACATTGGCAAAGATCTATCAATTGTTGGCGGGGAAATTATCTGGAAAAATGCTTGGAGAAGCTGCTAATTACCAGGTTAAGAAAGAGTTGTTGAAGAAGGGTGGGCAGTTCGCTTTGATCAATTTAGAATCCAGAACTGCTTTGCTTGCAGCAAAAAAGGGTTTTCTTGGTGCTGCATCAAGATATCTGGGTTTTAGAAGCATGCTCACATTACTTGGACCAGTGCTTTGGGGAACATTTCTGGCCGATCTGGTTATTCAAATGCTGGGAACAGATTATGCTAGAATCTTGCGAACAATTTATGCTTTAGCTCAGATTCGTGTCATCCGCACATATAAGTTGCCATCTGATATTGCCGAGGAATAGCCAAGTGGTTGGATTTTGAGCTCGTTCAAAATTTTGAAGTATCAATTAAGTCATTCCTCTTAATATCTCCATATTGGTCTTTATGCATATATATTGGCTGCTCAAGTCTTCTAAATATTGATTGGTCTTTAGTAAATAATTCAAAGATGGGGAATACTTAATTTTATATTCTATAATGCTATAATTTGTGTTGAAATANAAGTATTGTTTAGATTAAATTAGAGTTTTAGTTTAACTTATTTYYAAATTTCCCTTTGGTCTCTAAGTTTTTTTCATTTCAATTTGGTCCCATAGGATTCGTTCCACAAATTATTTTTTCTGGAGTTTCTCCTAGCGAAA
TARGET=135,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03986 Ps_contig_mira-and-tgicl-ass_franssen_31684
SEQUENCE=AGTACGCGGGGAGAGTTTATTAAAAAAATTGAGTGAAAGGACTAAAGTTGGAGAGTTATTGAATGAATTGGCATGGTAATAGCAATATGTTATTTTATTGTAAGATGAAAGTTGAGCCCTAAAAGGAGAAGATAGATGATGATGATGATGATGCGGTGTGGAAATGGCGCAAGTGGAGTGCTATTCATGCCATCATGCTTTTGCTCCTGTTGGAAATGGAAACTCTATCCTCCTCCTCCTACAACGGAATTGGTTTCGCAGAAACGCAATCGGCGTCGGAGTGTGAAGGTGTTTTGTCAAAAAAGGGAAAATGTTAATTCTTCTTATGACTTCGATCCCGAGCTACGCTCCGCTCTTGAACTTGCTACTGACTCTGAGTTATGTGAAATTCAAACCATCCTTTTCGGCCCAAGCTACTTGAGTCCTTTGCTCAAATCCATCACATTAACAACTGCACCAACAACTACCCTCGACGAACATGACATTCAACTGCGCCAACAATTTATAGCCACACTCGAGTCACGCTTTTTCTACTTAGCAGCTGATGCACGCTCAACATTAAGGGGTTGGAGACCGACTTATAGAAATGTCTTGCTTCACCTAAGGAATAAATTCAATATTCCTTGCTCAACCAAGCTCTCTACTCACGACCTTGAACTTGAAATATTCCTCCATCTTTTACACTATAACTCTAATCAAGAACCAGGAAACCATACAGCCTTATTGGATGTATCCACCCAGTCTGATGCTCAGGGAACTCTACAGCTTGGACTCAATCAATGGAAAATGCACTCAAAAGTCGGAACACAAGACTTTCAATCTCTTCTTTTCAAGGGAGGTGGTATACTTACATTGGCAAAGATCTATCAATTGTTGGCGGGGAAATTATCTGGAAAAATGCTTGGAGAAGCTGCTAATTACCAGGTTAAGAAAGAGTTGTTGAAGAAGGGTGGGCAGTTCGCTTTGATCAATTTAGAATCCAGAACTGCTTTGCTTGCAGCAAAAAAGGGTTTTCTTGGTGCTGCATCAAGATATCTGGGTTTTAGAAGCATGCTCACATTACTTGGACCAGTGCTTTGGGGAACATTTCTGGCCGATCTGGTTATTCAAATGCTGGGAACAGATTATGCTAGAATCTTGCGAACAATTTATGCTTTAGCTCAGATTCGTGTCATCCGCACATATAAGTTGCCATCTGATATTGCCGAGGAATAGCCAAGTGGTTGGATTTTGAGCTCGTTCAAAATTTTGAAGTATCAATTAAGTCATTCCTCTTAATATCTCCATATTGGTCTTTATGCATATATATTGGCTGCTCAAGTCTTCTAAATATTGATTGGTCTTTAGTAAATAATTCAAAGATGGGGAATACTTAATTTTATATTCTATAATGCTATAATTTGTGTTGAAATANAAGTATTGTTTAGATTAAATTAGAGTTTTAGTTTAACTTATTTYYAAATTTCCCTTTGGTCTCTAAGTTTTTTTCATTTCAATTTGGTCCCATAGGATTCGTTCCACAAATTATTTTTTCTGGAGTTTCTCCTAGCGAAA
TARGET=228,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03987 Ps_contig_mira-and-tgicl-ass_franssen_31699
SEQUENCE=GGGGGAAAAAAGTTTGAAAATAAAAATCGATTTTGTTACATTGCGGTTTTTACCTCATCTTCATCCTTCGCTACTTGCTCGAAGAACTTCGAGAGGGCAAGTTTCTTCTCCATGGATGCATCTCGGATCGACATCTTCGAGATTTACCGAAGATTCTGTGATATAAGATCAGGACATGCATTTGTTGTTGGTGAAGAAGGGTATAGACAAGATAGTGAGTTTCAGAAGGCTAAGGTCTCAAGGGAGGCATTGACACAGCTCTCGAAATTGGTGGAGTCTAGAGTTTGTACAGGGGCAACAATTTTTGATGAACTTTCAATGCTCATGTCACGGCTGGGTTTGATGGCAACCCTTTCAGAGTTCTCTCGGTTTTATGAATTTGTTTTCTTCATGTGTCGCGAAAATGGTCAAAAGAACATCACTGTAAGCAAGGCAGTGACTGCTTGGAAATTAGTTCTTAATGGGCGGTTTCCACTGCTTCGTCCATGGTGTGAGTTTATTGAGAAAAATCAACGATACAACATATCTGAGGATACATGGCAACAGGTTCTGTCTTTCAGCTTGTGTACGCGTGATAATCTCGATGCATACGATCCTGAAGGCGCTTGGCCTATTTTAATTGATGATTTTGTCGAGCATATGTACAGGCAACCGGGATCGTATTATGAAAATTCCAATTTCCAGTGTAATTGTGGTGATCCTGAATCTCTGTCAATTCCGGGACGGAAAAGTTTTGCTGGTCTAAAGAGGAAGTTAGCCGAGGAAGCAAGAAAAGATGATATGGAGTACTCTTATTCATCCGAGGAGATGAATTTGTCAAACTGTAAAAAGAGTAGATCACACGGTGTTGTAGATTGCGACGAGGAGAATCCACATGGGAATAATACACTTGAAGAATGCATGGATACAAGTAGACAGAGTAGCCCTTTGTGTTCATCTAAGTCCCATTGTGCAGTTGAAGGCTGTCTTTCAAAGGGTTTTGCAGGACTACTCTCAACTAGTTCCTATGTGCAGTTTGGTAGAGAGAGAAGACCTTCCTTTAGATGAAGAAGCACCCTCAATAGGTTAAGTATATACAAATTGCTTTTGCTATTTGGATGTATTTTATTTTGAATCAATAATAATTATGTTACTGAGAACTTCCTCATTGTTTCTGGTACTTGACTATTTATTTATTTATTTATTTTCTCACGTAATTAGAGTATATACAAAATGCTTTTGCGACTCGGTGT
TARGET=1166,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03988 Ps_contig_mira-and-tgicl-ass_franssen_31711
SEQUENCE=GTCGGGGTCGGGGGGTACCACTCAAAACTACAAGTTCATTTTTCAAACGGATAAGAAAATAAACCCAATGGCTTGCAATTCTACACCGCTCCTATCACCACCCAAAACAACCACCGTCGCAACCGTCCAACACCACCACCATCACCACACACTCCAACCAATCTTCCACACCAAACCCACCCTCACCCGGAAACTCACCACAAAACTCCACGTATCCTCCTCCCCAACCAACAAACCCACAACCACAACCACACCCTCACCCACCCAACCCCCACCCCCAAAACCCAATCAAGAAACCGTCTACTTCGACGGCGGAGCCCACTACGGCGACCTCGTCGCAAATCTCTTCCTAGGCTTCACCCTCGTATGGCTTCCCTTAACGCTAGCAGCAGTCTCACGTGCCCTTTACCTTCGTTACAGGTTCACCAACCTCAGAGTTACAGTAATCTCAGGGCTAACAGGCGAGGACAGAAGTGACTTTTCATACAGTGTTATCAAAGATGTTCAGGTTGTTCCAAGGTTCATTGGTGAATGGGGTGATATTGTTATCACATTGAAAGATGGTACTAAAGTTGATCTTAGGAGTGTTCCTAAGTTTAGAGAAATTGCCAAGTATTGTCTCTCTATGAGGGATAAATCTTCTCAGAATTTGAATAAATCTGCTCCTAAAGGCTTTGGAAAATAAATATTATACAGGTATTTAATGTTTATTATGTATACATACATATATATGTAAATTTATTTATTTGTTTTGTTTTATAGATTAATTTGGTGCTTGGCAATGTTAACATTTTGTATTGAATTTTGTTGTGCCACTTTTTTGATTCAATGATAATGGAATTTTGTTATTATGTGCANGATGGTAATGTATNACTAATTAAG
TARGET=737,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9183
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=1.910027
PRIMER_LEFT_SEQUENCE=CAAAGATGTTCAGGTTGTTCCA
PRIMER_RIGHT_SEQUENCE=GTTAACATTGCCAAGCACCAA
PRIMER_LEFT=495,22
PRIMER_RIGHT=788,21
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.910
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=2.1047
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=2.096482
PRIMER_LEFT_1_SEQUENCE=CAAAGATGTTCAGGTTGTTCCA
PRIMER_RIGHT_1_SEQUENCE=GTTAACATTGCCAAGCACCAAA
PRIMER_LEFT_1=495,22
PRIMER_RIGHT_1=788,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=62.096
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=2.1189
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=2.110660
PRIMER_LEFT_2_SEQUENCE=CAAAGATGTTCAGGTTGTTCCA
PRIMER_RIGHT_2_SEQUENCE=TAACATTGCCAAGCACCAAA
PRIMER_LEFT_2=495,22
PRIMER_RIGHT_2=786,20
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=292
PRIMER_PAIR_PENALTY_3=2.1189
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=2.110660
PRIMER_LEFT_3_SEQUENCE=CAAAGATGTTCAGGTTGTTCCA
PRIMER_RIGHT_3_SEQUENCE=TTAACATTGCCAAGCACCAA
PRIMER_LEFT_3=495,22
PRIMER_RIGHT_3=787,20
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=60.111
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=2.1370
PRIMER_LEFT_4_PENALTY=0.226971
PRIMER_RIGHT_4_PENALTY=1.910027
PRIMER_LEFT_4_SEQUENCE=ATGTTCAGGTTGTTCCAAGGTT
PRIMER_RIGHT_4_SEQUENCE=GTTAACATTGCCAAGCACCAA
PRIMER_LEFT_4=500,22
PRIMER_RIGHT_4=788,21
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=60.910
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_03989 Ps_contig_mira-and-tgicl-ass_franssen_31725
SEQUENCE=AACTTTCACAATTTCAATCCTTCTCGATCCTTTCTTTTCATTCTTCTYTCTTCTCCAGGTACATTTTCACCTTGTTTTTTTTTCTTAAATCAAACCTAATTTTTTCCGTAACGCAATTCGCTTATCGCATTGTTTTTTAATTTCTGTTATTCAGGGACTTTAATATACCCTCTCGCTTCGTTCTTCCAGATCGGTTGATCAATCCATAATGTCTCATATCCTTCGTCATTGTTCTAGCGGATTTTATTTTATTTTATTTTTAATTTATTTAATTTTGGTAATATTAGGGTTTTGTGATTTTGTTATTTTGTTATTATGATATGAAATTGGTTTTGTTTGGACTTAACTGAAGCGAACGCGGAAATCAAAGAGAAAAGGATCGCGAAAGGGCTCAAGCTAGAGCCAATAGCAAATCTAAGCGAGAAAAGAGTGATGGATTGACACCAGAACAAAGACGAGTAAAGGTTTTTTTTTGTTTGATGGTTTATTTTGTAGTGATAGGTGTGGGGGT
TARGET=241,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03990 Ps_contig_mira-and-tgicl-ass_franssen_31726
SEQUENCE=AAGGTTTACGTATCAYTNAATAATACCATGCTTTAATAAAAGAGCACAAACTATTAGCAATAGAAAATAGTTACAATCATACTAAACTTGTATGAACACAACCTCTAAAGACTAAATAGTTGTATGTACACATTTGTATGCTGCCTAAATCCTGGTGTATGTTATGTTCAGGTTCGGGATTCGGATCTGGCTTCACTGGCGCGCCTTGGGTACAAAGATCCTGCAGCTGGAACTTCAGCATGTGAAGGTACCATTGCTGAAAGAGGTTGGAAATTTGTATTTCCAACAAACCAATAGAGAGCCCATTTTAAATTGCAGAGTGTGTATTTGAGTGCTTTCGTCCAATTTTCCTGCTTATTGAAGCTTTGGGTGATGGAATAACTTTCCACTTTGTCACTCTCAATCTTATAAGGTAACTTGAAACATTTCTCAGGTGGTATGTTATTCTCTTGATCCTTAGATTTGGCAAATTCTGCAAAATCCTTAAGACATGCTAAAAAGAATGTCATTGCTCTGTCATAGCGAGTACTCCAGAACAAGTTGACTGGACCAAAAAGCTCTAAAGTAGTATTGTTGGTGTCTATGATTCGGGGATAGCTACCCATAGGAATTATTTTTATTCGATATTGAAACTTCGGTCGGAAATACTGGCACATTGTATGAAGAAGAAGGCATGCTTGGCCCCAGGCAGCATTTATCTCGTCCCATTCTACTGGAATTTTGGGGAGTCTTCCAAGCCGAAAATTGTTAATTGTTCCAAACTCTCCATCATGAGATATGGGAAAGGCGTCATTGAGCACATTTGTTCTCTTCAACAACTCTAAATGTGCTTGTGAAACTTCAATCTTAGCCAAAATTGCGTCTCTCTCTTCCTGATGAGAAATTAACTGAAACTGAAAATTATTGAACTCATGCCAATACCGCTCCTCCAATTCTTTGAAACGGGTAGATTTTAACTCAAGTTCCTTCCGTTCAGCATTTACTTCAGCATTTTGTCTCTCTGTTTCTTCAATTGCAGCTTCTAGTCTTCTTTCTTCTTCTTCAATCTTCATTTTCTCCTTAAGAAAATCAGCCTCGCTGAGGACATCTTTGGCCTCCCCCTCCAAGCGTTTAAGACATGCCTCATATGCTTCAATGTCCCTATTTACATCTTCGACCTCTTTATCAAGCTTATCAGACAAAACCCTCATGCAGTCAAGGCATAAAGGTTGTTCAACCTGTGTGTGTGTCGTGGCAATCTCAAATGCTCGGGTCAGAACAGTAATAGTTGAATTGAATCCAGAGTTATGAGGTGGCAAATGACCGCCATGATCAACCCCTGGGGCCGATGAGTTTCCCCCACTTCCATCAGTTGCTGATTCCGACTTGTACACAACCACAAATGACTCTTCCATTGTCTTTCCAGGCTGCGAAGTATCACCACGAGGCCGAGGAGGAACTCCTTGAGCCTGAGGTCTTTGCTGCTTTGGCAGCACAACATATGAGTTGTCCATTTTAGTTGTACTCATCACACTGCTAGCTCCATGAATGGATGAACCTTGCATCCCAGAACGAGAAGGGTCGTTGAAGAATTTGTCGGCGTAAGAATCGACGCCAACGACGCAAAGTGGATTGCGGCAGTTCTGACAAATCCATCGGGGGACATTCGGATCCACCGGAAAGCTCCGATTCCGACCCTTTTTCGTATCTCTCATTCTCTCTTGCTCAATCAGAAAATCAACCTTTTTAATTTTTATTTAATTTCCGAACCCTAATTCTTTTTTCTATTTTCGTCCCCGCGTACTCTGYNNTNANGTTTCTCCTTC
TARGET=1032,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03991 Ps_contig_mira-and-tgicl-ass_franssen_31726
SEQUENCE=AAGGTTTACGTATCAYTNAATAATACCATGCTTTAATAAAAGAGCACAAACTATTAGCAATAGAAAATAGTTACAATCATACTAAACTTGTATGAACACAACCTCTAAAGACTAAATAGTTGTATGTACACATTTGTATGCTGCCTAAATCCTGGTGTATGTTATGTTCAGGTTCGGGATTCGGATCTGGCTTCACTGGCGCGCCTTGGGTACAAAGATCCTGCAGCTGGAACTTCAGCATGTGAAGGTACCATTGCTGAAAGAGGTTGGAAATTTGTATTTCCAACAAACCAATAGAGAGCCCATTTTAAATTGCAGAGTGTGTATTTGAGTGCTTTCGTCCAATTTTCCTGCTTATTGAAGCTTTGGGTGATGGAATAACTTTCCACTTTGTCACTCTCAATCTTATAAGGTAACTTGAAACATTTCTCAGGTGGTATGTTATTCTCTTGATCCTTAGATTTGGCAAATTCTGCAAAATCCTTAAGACATGCTAAAAAGAATGTCATTGCTCTGTCATAGCGAGTACTCCAGAACAAGTTGACTGGACCAAAAAGCTCTAAAGTAGTATTGTTGGTGTCTATGATTCGGGGATAGCTACCCATAGGAATTATTTTTATTCGATATTGAAACTTCGGTCGGAAATACTGGCACATTGTATGAAGAAGAAGGCATGCTTGGCCCCAGGCAGCATTTATCTCGTCCCATTCTACTGGAATTTTGGGGAGTCTTCCAAGCCGAAAATTGTTAATTGTTCCAAACTCTCCATCATGAGATATGGGAAAGGCGTCATTGAGCACATTTGTTCTCTTCAACAACTCTAAATGTGCTTGTGAAACTTCAATCTTAGCCAAAATTGCGTCTCTCTCTTCCTGATGAGAAATTAACTGAAACTGAAAATTATTGAACTCATGCCAATACCGCTCCTCCAATTCTTTGAAACGGGTAGATTTTAACTCAAGTTCCTTCCGTTCAGCATTTACTTCAGCATTTTGTCTCTCTGTTTCTTCAATTGCAGCTTCTAGTCTTCTTTCTTCTTCTTCAATCTTCATTTTCTCCTTAAGAAAATCAGCCTCGCTGAGGACATCTTTGGCCTCCCCCTCCAAGCGTTTAAGACATGCCTCATATGCTTCAATGTCCCTATTTACATCTTCGACCTCTTTATCAAGCTTATCAGACAAAACCCTCATGCAGTCAAGGCATAAAGGTTGTTCAACCTGTGTGTGTGTCGTGGCAATCTCAAATGCTCGGGTCAGAACAGTAATAGTTGAATTGAATCCAGAGTTATGAGGTGGCAAATGACCGCCATGATCAACCCCTGGGGCCGATGAGTTTCCCCCACTTCCATCAGTTGCTGATTCCGACTTGTACACAACCACAAATGACTCTTCCATTGTCTTTCCAGGCTGCGAAGTATCACCACGAGGCCGAGGAGGAACTCCTTGAGCCTGAGGTCTTTGCTGCTTTGGCAGCACAACATATGAGTTGTCCATTTTAGTTGTACTCATCACACTGCTAGCTCCATGAATGGATGAACCTTGCATCCCAGAACGAGAAGGGTCGTTGAAGAATTTGTCGGCGTAAGAATCGACGCCAACGACGCAAAGTGGATTGCGGCAGTTCTGACAAATCCATCGGGGGACATTCGGATCCACCGGAAAGCTCCGATTCCGACCCTTTTTCGTATCTCTCATTCTCTCTTGCTCAATCAGAAAATCAACCTTTTTAATTTTTATTTAATTTCCGAACCCTAATTCTTTTTTCTATTTTCGTCCCCGCGTACTCTGYNNTNANGTTTCTCCTTC
TARGET=1219,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03992 Ps_contig_mira-and-tgicl-ass_franssen_31750
SEQUENCE=ACCGCTTAGAATCARAGACATAGATAGTTATTAGATCTAAAACGAAAAAAAGAAGAAGAAGAAGAAGAAGCTCTTTATCAAAGACGACAATGCCTTCAACTGAAGGAGACTTAGATCTTAGGCAATCTTCTCATTCTGCTTTCTCACCGGAGAAAACGCATTCGCCGATGTAGGAAATTTTGGAACATTGCGCCAAGTATTTGAACCAATCGCTCGTCACATTTGGTTTTTCTGCTTCGCTCGATCTTTTTGCGAACGATCCTGTTTCTATAGCGAGGACTTGCAATTGTATATACTTTTTGCTTCAGCAGAGACAGCGTGATGTTGAGTTTAGAGAGTCTGCTAATGAACAAAGACAACGATTGTTATCTGATATATCGAGATTTGAGGCCAAAGTTGAGAGACTTGAAGGGCAAATACAAGCCAAAGATAGAGAGATAGCAACGTTAACTAGGAAGGAAGCTAAAAACACTTCAGCTCTTAAGGCCACAATTGATAAGCTGCAACAGGAGAGGGATGAATTTCAGAGGATGGTTATTGGTAATCAGCAAGTAAAGACTCAACAAGTACATGAGATGAAGAAGAAGGAAAAGGAATACATAAAACTGCAGGAGAGACTAAACCAAGTGTTGATGGAGAAGAAGAAAGAATCTAGGTCGGGCATGGAGATAATGAATTTGCTTCAGAAGGAAGGGAGGCAACGTGGGACTTGGAATGGGAAGAAAACAGACAATGATTTTTATAAAAAGATTGTGGATGCTTATGAGGCAAAGAATCAAGAACTGATAGCAGAAAATGCTGATTTAAGAGCATTATTGAGATCAATGCAGGTGGATATGCGTGATTTCTTAAATGCTCCTAATGGGCTGCCAAATCAATCTCCCACTCCAAATGAAAGAGCTGAAAGTAATCCTTCGCAATCGCCGCTGGTTGGGAGAACGGACGTATTTGATCTTCCTTTTCACATGGCCAGAGATCAAATTGAAGAGAGTCTTAGAACCAAGATGGCTTCTATAAAGGAGCGCATGGTCCAATTGCAAGATGCGCAGAAAGAGGCAGACGTCACTTCGGAAGCTACCGAGAGGGAACTTGAGCTTGAAGCTCAACTTGTTGAAGCAAGGAGCATTATACAAGAGCAGGAATCTATAATGGCTAAACATCTTTCAAAGTCTGACAGACCAAGGGAATCGGCACCGGCTGAGGGAGTATGTAACTAACATTGTAACCAGGTCTCATGCTTTCATGATTACTTACATTGGCTCCATGTAACTAATCTTGCTTGTCCATTCTGAATTACCCCCTTAGTTGAGGATGTAACAACAGGCCCTCCAAGTCGAATGTCATTGTTATAAGTATGATATTGTAATTAAGTGATATTGTAGACTTGGAACTGCTTCCGGAAGAAACTAGTTATTTATTTCCGGAGGATCACTCAAACTAGCATAGCATTTAAGATTTGATTTGTAGCTAGAAAGATTTCAAGAGTTAGTAATTATAAAGTATGATTCAGTACAGTTTTACTAATTTACT
TARGET=50,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03993 Ps_contig_mira-and-tgicl-ass_franssen_31751
SEQUENCE=AGGAGTACTGCTGGAAAAAGAATGGTTTGATGCCTGCTCAAACCATGACCCCTGAGCTGCAGAGAAATCTGAAGTTACTAAAGTTGAGGGATGCCATTGATCCAAAACGGCACTACAAGAAGGGTGATTCAAAATCAAAGAGTCTTCCAAAATACTTTCAGTTGTAGGTTCTCCGCTGGACTACTTCTCAGGCAGATTAACAAAGAAAGAAAGGAAAGAAACGTTTGCAGATGAGTTG
TARGET=202,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_03994 Ps_contig_mira-and-tgicl-ass_franssen_31758
SEQUENCE=GCAGAGTACGCGGGGACGTCACTATAATAGCGTACTAGATTTGGTTCTGTGACTACCGTGTCCCATAGATTTGCATAACAATGATCTCTTCCTTAATGTTACCATCTGCTACAACATTAAGACCTTGTTGTAGCTGCAGCATCATTCCCTCATCATCATCATCTTCATCTTCACGAAGACTCACTCTCTTTCATCTTCTCACCACCGGTGCTATTGTTCTTCCACCACAATCACAATTCTTCGGTGCAAAACTATCACAAGCTAATGCTGCTCAAAACTTGTTTGATTGGGTCCAAAATGATGATAGAAGATTTCTACATGTTGTTTACAGTGTTGCAGATTTGGACAAGACTATAAAGTTCTACACTGAATGTCTTGGCATGAAGCTTTTGAGAAAACGCGATATACCGGAAGATAAGTATTCGAATGCTTTTCTTGGATATGGACCTGAAGATTCCAATTTTACAGTGGAACTTACTTATAATTATGGTGTAGATAACTATGACATTGGAACTGGTTTTGGACATTTTGGTGTTGTAGCTCAAGATGTTGCCAAGACAGTTGATATTGTAAAGGCGAAAGGGGGCAAGGTTACCAGGGAGCCGGGGCGTGTCAAAGGCGGCTCTACAGTTAGTGCTTCCGTTGAAGATCCTAGTGGTTATCGGTTTGAGCTTTTGGAGAGAAGACTTACTCGTGAACCGTTGTGCCAAGTGATGCTTCGAGTAGGCGATCTTGATCGTGTCATAGCCTTCTATGAAAAAGCTGTTGGAATGAAACTTCTCCGCAAGATAGACAACCCTGAGGAAAAGAACACAGTAGCCGTGTTAGGATACGGTCCTGAAGCTAATGGTCCAGTTCTAGAACTGACGTATAACTACGGCGTCACTAATTATGACAAAGGAAATGGTTATACACAGATCGCAATAGGCACGAATGATGTCTATAAGACAGCAGAAGCAATCAAGTTATACGGAGGTAAGATTATCCGTGAACCTGGACCTTTACCAGGTATCAACACCAAGATTGTCGTTTGCTTAGACCCTGATGGTTGGAAATTGGTATTTGTTGATAATGTCGATTTTCTGAAGGAATTAGAGTGACAAAAGCATGGATTTTATAGATCCTACACTTCGCTGAACTTATAATAGGCCAACAATTTTTTCAGCGATATTTTATAATCGCGCTGTCAATTCAACATATCGATAAAGAAAAGTAAACATGTATTTACAGCAATCACATTGTCATCTTTAATTATTTTTTAGTATGTTACTTTAGCGCGGCAACTCAACRTGTATTGGTATTCCTTCTAAAGAAA
TARGET=150,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03995 Ps_contig_mira-and-tgicl-ass_franssen_31762
SEQUENCE=CTTTCTTTTTCTTTCTTTTATACGACATTCACTTACTATTCATGTACTGCAAAGCTACCCCAAACTTAAAGGTTGCTATTCCAACAGCAACCCCAAACTTAGATAGAAACGTAGAGATGARATCAATTCTCACATACTCTGAACAGGGTAGGATAGGTACAAGGTCATGGCTTTAGAAAAAAAAACGGGTATATCAANAATAAATGATTAACAGGCTCAACAGGGTAAAACAATGGATAATAATAATAATGGGATGGCTAGAAAGGCTCTAGGTGATAAACAAAAACATGCCTCAGTGTGTGTATTCGTACAGCTAATAATAACAAAAGAACGTACGCAAACCAAGATTGATAAAGACATACCTGATATCTCTCATATGATGATAAGTGTTTGGCTTTTTATGATCTCACCAGGACGGGTTAAGCTGACTTGTTCTATCATACCTCTGAGTTCAAAGCTCATGCTCGTGGTTCTGATGTTAATCTTAACCAGTGCGTCCAATCATAAACAACAGTATCTACAGTCAGGGGACTGAAAGAGAGGACGCCCCAAGTAGTTGGTGTAAGTGATCAAGAAAACCAATAGCCAGACATAGTCACGTATCTAATGAAATAAACAGGAAACTGGAGGTAAACCAAATCAAATTCATTAGAATAAGCCAACCCATAATAGGTGGTTACATCAAAGCAAACARAAAATTAAAAAACTGAATAAACT
TARGET=237,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_03996 Ps_contig_mira-and-tgicl-ass_franssen_31769
SEQUENCE=CAGTGTCCCTTGTTGGGAATACTTTAGTANTTTTGGTGGGCAAGATGCAAAAAGGGCTCTCTTGAATGACCTGCATATTCTGGACTTGGAAACTATGACTTGGGACGAAATTGATGCCGTTGGAGTGCCTCCTTCCCCAAGGTCTGATCATGCTGCTGCTGTACATGTGGAGCGATACTTGCTCATCTTCGGAGGGGGTTCACATGCAACTTGCTACAATGATTTACATGTTCTCGATTTGCAAACTATGGAATGGTCTCGCCCCACACAACAAGGTGAGATACCAACTCCACGTGCAGGACATGCTGGTGTGGCAGTAGGAGAGAACTGGTTCATTGTTGGTGGTGGCGACAACAAGAGCGGGGCTTCGGAGACGGTTGTACTGAACATGTCTACACTGGCTTGGTCAGCAGTAACATCTGTTCAAGGGCGTGTTTCTGTTGCTAGTGAGGGCTTGAGTTTGGTTGTAAGCTCGAATGATGGAGAAGATGTACTTGTATCTTTTGGAGGATACAATGGACGTTACAACAATGAAGTATATGTTCTTAAACCAAGCCACAAATCCACCTTGCAATCCAAAATAAATGAAAATTCCATACCAGACAGTGTTTCTGCTGTACCAAATGCTACAAATGCTACTCGAGATTCGGAGCCTGAACTTGGAGCAGGACAAGAAGGGAAAATTTGGGAAATTACTGTGGACAACGCTTATCCAACAAAATCCAAAGGTGATATCCTATCAGTACTAAAGGCTGAGAGAGATGAATTAGAATCATCGCTTGGTAAGGAGAGGCTGCATACTCTTCAACTAAAGCAAGAGCTGGCTGATGCCGAGTCTAGTAACTCTGACCTTTACAAGGAGCTCCAATCAGTACGAGGTCAGCTTGCTGCTGAGCAGTCAAGGTGTTTCAAACTTGAGGTGGAGGTTGCTGAATTAGGTCAGAAGCTCCAAAATTTTGGGACTTTACAAAAGGAGCTTGAACTCCTCCAGCGGCAAAAGGCTGCATCAGAGCAAGCTGCTTTAAATGCAAGACAGAGGCAGGGTTCAGGTGGCGTGTGGGGTTGGCTTGCCGGTTCTCCCCCTGATCCGAATGTAGATGATGAATGAACCTTGGCTTAAGCTAAACAGCAACGCATCATTTGGTTTCATTGTGCCAATTTTATTCTCAATACTTGTTGTGTATTAATTAATTAATTCGGTTCTTTTAGTGATGTGAATAAGATTTAGCTTCTATTTGGGCGGAGGAAGATTTTCTACACATGAGGATATGATAGTATGATATAGTGAAGATTATTTTTTCACCATTTTTGCTGATGACTGTATTCAAAGTGGATAATATACACTATTAC
TARGET=1183,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0691
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=AGATGATGAATGAACCTTGGCT
PRIMER_RIGHT_SEQUENCE=ACAGTCATCAGCAAAAATGGTG
PRIMER_LEFT=1095,22
PRIMER_RIGHT=1321,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.2107
PRIMER_LEFT_1_PENALTY=0.173391
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=CCCTGATCCGAATGTAGATGAT
PRIMER_RIGHT_1_SEQUENCE=ACAGTCATCAGCAAAAATGGTG
PRIMER_LEFT_1=1080,22
PRIMER_RIGHT_1=1321,22
PRIMER_LEFT_1_TM=60.173
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.3648
PRIMER_LEFT_2_PENALTY=0.327468
PRIMER_RIGHT_2_PENALTY=0.037339
PRIMER_LEFT_2_SEQUENCE=GATGATGAATGAACCTTGGCTT
PRIMER_RIGHT_2_SEQUENCE=ACAGTCATCAGCAAAAATGGTG
PRIMER_LEFT_2=1096,22
PRIMER_RIGHT_2=1321,22
PRIMER_LEFT_2_TM=60.327
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.4575
PRIMER_LEFT_3_PENALTY=0.420194
PRIMER_RIGHT_3_PENALTY=0.037339
PRIMER_LEFT_3_SEQUENCE=TTAAGCTAAACAGCAACGCATC
PRIMER_RIGHT_3_SEQUENCE=ACAGTCATCAGCAAAAATGGTG
PRIMER_LEFT_3=1116,22
PRIMER_RIGHT_3=1321,22
PRIMER_LEFT_3_TM=59.580
PRIMER_RIGHT_3_TM=60.037
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.9621
PRIMER_LEFT_4_PENALTY=0.924791
PRIMER_RIGHT_4_PENALTY=0.037339
PRIMER_LEFT_4_SEQUENCE=TAAGCTAAACAGCAACGCATCA
PRIMER_RIGHT_4_SEQUENCE=ACAGTCATCAGCAAAAATGGTG
PRIMER_LEFT_4=1117,22
PRIMER_RIGHT_4=1321,22
PRIMER_LEFT_4_TM=60.925
PRIMER_RIGHT_4_TM=60.037
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_03997 Ps_contig_mira-and-tgicl-ass_franssen_31773
SEQUENCE=CAACGCAGAGTACTCCGGGGGTCCACACAACCCTCTCTCTTCTCTCTCACTCCTCTGAAAAGATAAAACAAAAAAACACCTTTCCTCTTCTTCACTCCACAAGACCCACGCGACCTCACCTTCTCCCTAACCACAAACGGCGCTCACCCTTCACACCGCTTAACCTACCTGCCTCCTTCGTCACACACCACCAAAAACCTCACAACCGTTCCTCTCCGTAATCACCAAAATCACCGTTAAACACCTCTGTCTCCAACCTCCGCTTCACTGTTCATCATATCGCATCCGCCATGGAACCTCACGATTTCCGGAATCACCAAATCACCCTTCTGTTCATCACCTCAACATGATTTCAGATCTCTACCGCCGTTTCTCCATCAAAATCCAATCGCCGCCGTGACGAACCGGAGCAAGACGGAAATGGAAGTTTGGCGGTGGTGGATAGTAACGGTAGTGATATTGTTAAGAGGACCGAGGGGAGGATTGTGCTGACTGAACTTCATAGAGAGGCACAGAAGCTTACATGGCGTATGCTATGTCGGTGTTGCTTGGTCGTGCTTCGCCTGATGTTCGTGATGGGTTGAAGCCTGTTCATAGAAGGATTCTATTTGCAATGCACGAGCTTGGCCTTTCGTCCAAAAAACCATTCAAGAAATGTGAAAGAGTTGTTGGAGAGGTATTGGGAAAATTTCACCCTCATGGTGACTCTGTTGTATATGATTCCATGGTTCGGATGACCCAGGATTTTTCATTACGAAGTCCTTTGGTCAATGGTCATGGAAATTTTGGGTCAATTGATGTTGACCCTCCTGCCGCCATGCGTTACAGAGAGTGCAGATTGGAAGAATTGGCTGAAGCAATGTTGTTGGTAGATCTTGATCAAGATACAGTTAATTTCGTTCCAAATTTTGATAACTCACAGAAAAAGCCATCGATCCTAAGCGGCGCCTCCTTCCACTGTTCCCTTCTAACCTTAAACTCAACCTCCTTCAACTGCTACAACAACCAAACATCGACGCAACTTGTTTCAGCAACATCAACATTTTACTGTGCTTTCAATAAGCAGAACGATTCTGTTTTTGGTAAATTTTCAATCTGAAAACCTTGTTGCATTCTAAGTTTTATTTATTTATTTATATTTTAACTATTTTACGTTTGTGTATGCACTAACTTTATGTGTGCTGTTAGGACGATACATTATGTATACGTTTCGGTTGCGTTGCACACTATTTTCTTGTTTTGAGAATGTCATGGCAGTTTGTAGTTCAGTAAATATTGTTTGATATGATGTTGCTTTGATTAACCTGTGTCGACTATTCGACAATTTCCAACAACTATTCAACAGTGGTAATGATCCAA
TARGET=1121,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1700
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.083617
PRIMER_LEFT_SEQUENCE=TCAACCTCCTTCAACTGCTACA
PRIMER_RIGHT_SEQUENCE=CGCAACCGAAACGTATACATAA
PRIMER_LEFT=979,22
PRIMER_RIGHT=1218,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=59.916
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.1861
PRIMER_LEFT_1_PENALTY=0.102490
PRIMER_RIGHT_1_PENALTY=0.083617
PRIMER_LEFT_1_SEQUENCE=TCCTTCCACTGTTCCCTTCTAA
PRIMER_RIGHT_1_SEQUENCE=CGCAACCGAAACGTATACATAA
PRIMER_LEFT_1=949,22
PRIMER_RIGHT_1=1218,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=59.916
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.2870
PRIMER_LEFT_2_PENALTY=0.203343
PRIMER_RIGHT_2_PENALTY=0.083617
PRIMER_LEFT_2_SEQUENCE=CCTTCAACTGCTACAACAACCA
PRIMER_RIGHT_2_SEQUENCE=CGCAACCGAAACGTATACATAA
PRIMER_LEFT_2=986,22
PRIMER_RIGHT_2=1218,22
PRIMER_LEFT_2_TM=60.203
PRIMER_RIGHT_2_TM=59.916
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.2888
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.083617
PRIMER_LEFT_3_SEQUENCE=AAACTCAACCTCCTTCAACTGC
PRIMER_RIGHT_3_SEQUENCE=CGCAACCGAAACGTATACATAA
PRIMER_LEFT_3=975,22
PRIMER_RIGHT_3=1218,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=59.916
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.3382
PRIMER_LEFT_4_PENALTY=0.254567
PRIMER_RIGHT_4_PENALTY=0.083617
PRIMER_LEFT_4_SEQUENCE=CTCCTTCCACTGTTCCCTTCTA
PRIMER_RIGHT_4_SEQUENCE=CGCAACCGAAACGTATACATAA
PRIMER_LEFT_4=948,22
PRIMER_RIGHT_4=1218,22
PRIMER_LEFT_4_TM=59.745
PRIMER_RIGHT_4_TM=59.916
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_03998 Ps_contig_mira-and-tgicl-ass_franssen_31781
SEQUENCE=AATTTTGAAAGAAACACGAAGGGTGTAGTAAATTTCCATTTTCAAACAAAAACTCTCTCTCTCTGGTACAAGTACAACTACAACAAGAACGATTCGGTGGTGGAATCAAAAGTGTGCGATTCAATTGAATTGAACAAATCGATTGGGTTTCTTTGAATGGAAGCTATCAGAAAGCAAGCATCGAAGCTTCGAGAACAAGTCGCTCGCCAACAACAGGCTGTGACTAAAACAGGTTTGGGGCTGGGGATATGGAGGTTCGGAATTGGACTTGGAGC
TARGET=53,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5545
PRIMER_LEFT_PENALTY=0.351175
PRIMER_RIGHT_PENALTY=0.203343
PRIMER_LEFT_SEQUENCE=TGAAAGAAACACGAAGGGTGTA
PRIMER_RIGHT_SEQUENCE=TTTAGTCACAGCCTGTTGTTGG
PRIMER_LEFT=5,22
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=59.649
PRIMER_RIGHT_TM=60.203
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=223
PRIMER_PAIR_PENALTY_1=0.7421
PRIMER_LEFT_1_PENALTY=0.351175
PRIMER_RIGHT_1_PENALTY=0.390906
PRIMER_LEFT_1_SEQUENCE=TGAAAGAAACACGAAGGGTGTA
PRIMER_RIGHT_1_SEQUENCE=TAGCTTCCATTCAAAGAAACCC
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=165,22
PRIMER_LEFT_1_TM=59.649
PRIMER_RIGHT_1_TM=59.609
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=161
PRIMER_PAIR_PENALTY_2=1.1159
PRIMER_LEFT_2_PENALTY=0.912551
PRIMER_RIGHT_2_PENALTY=0.203343
PRIMER_LEFT_2_SEQUENCE=TTGAAAGAAACACGAAGGGTGT
PRIMER_RIGHT_2_SEQUENCE=TTTAGTCACAGCCTGTTGTTGG
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=227,22
PRIMER_LEFT_2_TM=60.913
PRIMER_RIGHT_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=1.3353
PRIMER_LEFT_3_PENALTY=1.131978
PRIMER_RIGHT_3_PENALTY=0.203343
PRIMER_LEFT_3_SEQUENCE=TTGAAAGAAACACGAAGGGTG
PRIMER_RIGHT_3_SEQUENCE=TTTAGTCACAGCCTGTTGTTGG
PRIMER_LEFT_3=4,21
PRIMER_RIGHT_3=227,22
PRIMER_LEFT_3_TM=60.132
PRIMER_RIGHT_3_TM=60.203
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=1.3456
PRIMER_LEFT_4_PENALTY=0.351175
PRIMER_RIGHT_4_PENALTY=0.994428
PRIMER_LEFT_4_SEQUENCE=TGAAAGAAACACGAAGGGTGTA
PRIMER_RIGHT_4_SEQUENCE=TGCTTGCTTTCTGATAGCTTCC
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=179,22
PRIMER_LEFT_4_TM=59.649
PRIMER_RIGHT_4_TM=60.994
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_03999 Ps_contig_mira-and-tgicl-ass_franssen_31783
SEQUENCE=TAACGACACGAGTGCTTTCCTTCTTTTTCCCATCTTTTGCTTTCTCTTCCCTCCCAATTCTTTCTCTCTCTTTCTCTCTCTCTCTGACCTAATTGTCTCTCTTCTATTCACATAACCACTCATCTCCACAAACACACCAACCACAACCTTGAGAAGAAGATCCTTCTAAATTTAGTATCTTTCTTTCATATTCTCCAACCCCATCACCTAGATCTGTCTATAGACTCATAAAAAAACAACATTTGGGAAGTTTTGTCACAGCTAGCTATATCTCTAGAGGATAATATTGCAATAATATTAACAAGATCTTCATCAAGATGACTCCAATTAACTTGGCGGGACAGTTTGGTGACACGACATACACCAAGGTTTTTGTCGGAGGTTTAGCTTGGGAAACTCAGAAAGAAACCATGAAGAAGTATTTTGAACAGTTTGGTGAGATCTTGGAAGCTGTTGTCATCACTGATAAAGCTACCGGAAGATCCAAAGGCTATGGATTTGTTACTTTTCGTGAACCTGATGCTGCTATGAGAGCTTGTGTTGATGCTGCTCCTATAATTGATGGAAGAAGAGCTAATTGCAATCTTGCTTCTTTGGGTGTTCAAAGATCTAAGCCTTCAACACCAAAGCATGGAGGAGGAGGAAGGAACTTTAGGGTAATGGGTTCATTCCAGACAGGATTTGGAGGAGTGGGATCAGCTTTTCCTTCAGCTGCAACCTTCCCTCATTATGCTATCCAGCAAGGGATACCTTATAATGTTTATGGGTACTCTTCTTACTCGCCAGATTATAGTTACCCTACGAGCTACTATGGTGTGTATGGTGGAGCAACAGCACAGTATCCAGTTTATGGAAGTGGTCCAACTGGAGGGATGATGACTGGTGGTGCGGCGGCCGCCGCAGCTTACTATCCATATCTTCAATACGGCGAAGGTACCGGCGGTGCAACGACAGGCGGCTACACTTCCGGGCAAGGTTATGGTGTTAACTACCCACCTCATGTGTTTCAATATTCTCCAATTGCTTCTACTGGTGGTTATGCTCAGCATTATGGAACTCCCATTTCTCTTGCTCCTTCTCCTGCTGCCTTCGCCATCAGTGYGTTTTGNCTGTGCCACAGGCGTGACAATGGCTCTTCAAACTCCAATTCCTCATCGTTAGAGACTCATCATATTCATATCATGATCTCAAAAAAATCTAGCTGTGTCAAAACTTCCCCAAGTTATTGCTTCTTGAAGTCTCTTATATCATCAATCAACCTTTGGCCTAACCATGGTGTTTCACACACACACACTTTTCACTAACACCC
TARGET=73,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04000 Ps_contig_mira-and-tgicl-ass_franssen_31783
SEQUENCE=TAACGACACGAGTGCTTTCCTTCTTTTTCCCATCTTTTGCTTTCTCTTCCCTCCCAATTCTTTCTCTCTCTTTCTCTCTCTCTCTGACCTAATTGTCTCTCTTCTATTCACATAACCACTCATCTCCACAAACACACCAACCACAACCTTGAGAAGAAGATCCTTCTAAATTTAGTATCTTTCTTTCATATTCTCCAACCCCATCACCTAGATCTGTCTATAGACTCATAAAAAAACAACATTTGGGAAGTTTTGTCACAGCTAGCTATATCTCTAGAGGATAATATTGCAATAATATTAACAAGATCTTCATCAAGATGACTCCAATTAACTTGGCGGGACAGTTTGGTGACACGACATACACCAAGGTTTTTGTCGGAGGTTTAGCTTGGGAAACTCAGAAAGAAACCATGAAGAAGTATTTTGAACAGTTTGGTGAGATCTTGGAAGCTGTTGTCATCACTGATAAAGCTACCGGAAGATCCAAAGGCTATGGATTTGTTACTTTTCGTGAACCTGATGCTGCTATGAGAGCTTGTGTTGATGCTGCTCCTATAATTGATGGAAGAAGAGCTAATTGCAATCTTGCTTCTTTGGGTGTTCAAAGATCTAAGCCTTCAACACCAAAGCATGGAGGAGGAGGAAGGAACTTTAGGGTAATGGGTTCATTCCAGACAGGATTTGGAGGAGTGGGATCAGCTTTTCCTTCAGCTGCAACCTTCCCTCATTATGCTATCCAGCAAGGGATACCTTATAATGTTTATGGGTACTCTTCTTACTCGCCAGATTATAGTTACCCTACGAGCTACTATGGTGTGTATGGTGGAGCAACAGCACAGTATCCAGTTTATGGAAGTGGTCCAACTGGAGGGATGATGACTGGTGGTGCGGCGGCCGCCGCAGCTTACTATCCATATCTTCAATACGGCGAAGGTACCGGCGGTGCAACGACAGGCGGCTACACTTCCGGGCAAGGTTATGGTGTTAACTACCCACCTCATGTGTTTCAATATTCTCCAATTGCTTCTACTGGTGGTTATGCTCAGCATTATGGAACTCCCATTTCTCTTGCTCCTTCTCCTGCTGCCTTCGCCATCAGTGYGTTTTGNCTGTGCCACAGGCGTGACAATGGCTCTTCAAACTCCAATTCCTCATCGTTAGAGACTCATCATATTCATATCATGATCTCAAAAAAATCTAGCTGTGTCAAAACTTCCCCAAGTTATTGCTTCTTGAAGTCTCTTATATCATCAATCAACCTTTGGCCTAACCATGGTGTTTCACACACACACACTTTTCACTAACACCC
TARGET=633,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04001 Ps_contig_mira-and-tgicl-ass_franssen_31783
SEQUENCE=TAACGACACGAGTGCTTTCCTTCTTTTTCCCATCTTTTGCTTTCTCTTCCCTCCCAATTCTTTCTCTCTCTTTCTCTCTCTCTCTGACCTAATTGTCTCTCTTCTATTCACATAACCACTCATCTCCACAAACACACCAACCACAACCTTGAGAAGAAGATCCTTCTAAATTTAGTATCTTTCTTTCATATTCTCCAACCCCATCACCTAGATCTGTCTATAGACTCATAAAAAAACAACATTTGGGAAGTTTTGTCACAGCTAGCTATATCTCTAGAGGATAATATTGCAATAATATTAACAAGATCTTCATCAAGATGACTCCAATTAACTTGGCGGGACAGTTTGGTGACACGACATACACCAAGGTTTTTGTCGGAGGTTTAGCTTGGGAAACTCAGAAAGAAACCATGAAGAAGTATTTTGAACAGTTTGGTGAGATCTTGGAAGCTGTTGTCATCACTGATAAAGCTACCGGAAGATCCAAAGGCTATGGATTTGTTACTTTTCGTGAACCTGATGCTGCTATGAGAGCTTGTGTTGATGCTGCTCCTATAATTGATGGAAGAAGAGCTAATTGCAATCTTGCTTCTTTGGGTGTTCAAAGATCTAAGCCTTCAACACCAAAGCATGGAGGAGGAGGAAGGAACTTTAGGGTAATGGGTTCATTCCAGACAGGATTTGGAGGAGTGGGATCAGCTTTTCCTTCAGCTGCAACCTTCCCTCATTATGCTATCCAGCAAGGGATACCTTATAATGTTTATGGGTACTCTTCTTACTCGCCAGATTATAGTTACCCTACGAGCTACTATGGTGTGTATGGTGGAGCAACAGCACAGTATCCAGTTTATGGAAGTGGTCCAACTGGAGGGATGATGACTGGTGGTGCGGCGGCCGCCGCAGCTTACTATCCATATCTTCAATACGGCGAAGGTACCGGCGGTGCAACGACAGGCGGCTACACTTCCGGGCAAGGTTATGGTGTTAACTACCCACCTCATGTGTTTCAATATTCTCCAATTGCTTCTACTGGTGGTTATGCTCAGCATTATGGAACTCCCATTTCTCTTGCTCCTTCTCCTGCTGCCTTCGCCATCAGTGYGTTTTGNCTGTGCCACAGGCGTGACAATGGCTCTTCAAACTCCAATTCCTCATCGTTAGAGACTCATCATATTCATATCATGATCTCAAAAAAATCTAGCTGTGTCAAAACTTCCCCAAGTTATTGCTTCTTGAAGTCTCTTATATCATCAATCAACCTTTGGCCTAACCATGGTGTTTCACACACACACACTTTTCACTAACACCC
TARGET=1282,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04002 Ps_contig_mira-and-tgicl-ass_franssen_31792
SEQUENCE=ACCGTACTCTCGGGAGTTACATGACAGATAAAACCTTCCCTTCAAACTTAGAGTCCATCATGTTCGGAAGTGTCTTGCAAGGTGTAGAATCTTCTAAAAGAAATTCACTTATCATCAAGCAAATAATGCGACAAATCGTTACATCTCTTAAGAAAATTCATGATACTGGCATTGTACACAGGGATGTAAAGCCTTCAAACTTGGTTGTTACAAAACAAGGACAGATTAAACTCATTGATTTCGGTGCAGCAACAGACCTTAGAATTGGAAAGAATTTTGTTCCTGATTATACACCTTTGGATCCTGATTATTGTCCACCGGAACTATATGTGCTACCACAAGAAACACAAAGTCTTCCTCCCGAGCCAATTGCAGCTTTACTTTCACCAATCTTGTGGCAGTTAAACAGTCCTGATCTCTTTGATATGTATTCTGCTGGAATTGTACTCCTGCAAATGGCAATACCAACCTTAAGGTCTCAAGCTGCTTTGAAAAATTTCAATTTGGAAATGAGAACATGCGGATACGATTTGAACAAATGGAGGGACTCTACTCGCATGAAGTCTAACTTCCAAATCCTTGATAGTGATTCTGGTAGAGGCTGGGACTTAGCGTCAAAGCTTATCTCCGAAAGAAGAAAGCGTTTATCCGCTGCTTCTGCTTTGCGGCATCCTTATTTTCTTCTCAGTGCTGATCAGGCAGCCGCCGTCCTTTCGAAATTCAGCTTTAGCACAAAGTGAGCTGCTTATAAATTTTCGGTTTGTTTGAATGATTTGATTGAGATTACATACATGTATAAACACCTGCACGACTATTTGAAAGAGCTTATAGAAACAACTTATAACTAAACAAAAAAAAAAAAAAAACAAAAAAAAAAAAACGTAAAAACTCTTCGTCGTTCGTATACCTAACCTACGTCTTCTTCGAGACGACGACGACGAAGGGAGGTAGACGACGACG
TARGET=928,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.7888
PRIMER_LEFT_PENALTY=0.193337
PRIMER_RIGHT_PENALTY=3.595439
PRIMER_LEFT_SEQUENCE=CACCTGCACGACTATTTGAAAG
PRIMER_RIGHT_SEQUENCE=CGTCGTCGTCTACCTCCCTT
PRIMER_LEFT=800,22
PRIMER_RIGHT=959,20
PRIMER_LEFT_TM=59.807
PRIMER_RIGHT_TM=61.595
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=60.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=3.9188
PRIMER_LEFT_1_PENALTY=0.323355
PRIMER_RIGHT_1_PENALTY=3.595439
PRIMER_LEFT_1_SEQUENCE=AAACACCTGCACGACTATTTGA
PRIMER_RIGHT_1_SEQUENCE=CGTCGTCGTCTACCTCCCTT
PRIMER_LEFT_1=797,22
PRIMER_RIGHT_1=959,20
PRIMER_LEFT_1_TM=59.677
PRIMER_RIGHT_1_TM=61.595
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=60.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=3.9188
PRIMER_LEFT_2_PENALTY=0.323355
PRIMER_RIGHT_2_PENALTY=3.595439
PRIMER_LEFT_2_SEQUENCE=AACACCTGCACGACTATTTGAA
PRIMER_RIGHT_2_SEQUENCE=CGTCGTCGTCTACCTCCCTT
PRIMER_LEFT_2=798,22
PRIMER_RIGHT_2=959,20
PRIMER_LEFT_2_TM=59.677
PRIMER_RIGHT_2_TM=61.595
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=4.1530
PRIMER_LEFT_3_PENALTY=0.557601
PRIMER_RIGHT_3_PENALTY=3.595439
PRIMER_LEFT_3_SEQUENCE=CGGCATCCTTATTTTCTTCTCA
PRIMER_RIGHT_3_SEQUENCE=CGTCGTCGTCTACCTCCCTT
PRIMER_LEFT_3=665,22
PRIMER_RIGHT_3=959,20
PRIMER_LEFT_3_TM=60.558
PRIMER_RIGHT_3_TM=61.595
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=60.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=4.8816
PRIMER_LEFT_4_PENALTY=1.286198
PRIMER_RIGHT_4_PENALTY=3.595439
PRIMER_LEFT_4_SEQUENCE=GCGGCATCCTTATTTTCTTCT
PRIMER_RIGHT_4_SEQUENCE=CGTCGTCGTCTACCTCCCTT
PRIMER_LEFT_4=664,21
PRIMER_RIGHT_4=959,20
PRIMER_LEFT_4_TM=59.714
PRIMER_RIGHT_4_TM=61.595
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=60.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_04003 Ps_contig_mira-and-tgicl-ass_franssen_31794
SEQUENCE=ATTGAGCACTCCAATCATCTTTCGTTTACGGGTTATTTTAGTTTTCTAGTCTATTCTTCTAACGGAAAATCGAAAAAATGAAGTTCATCGAAAATCTCATCCGACAATCCATTAAAATTCTTGTCGAAAAACCAAGAGTCCTTCATGTTAGGAATATCCTCAGGCGTAGTAGTGGATGAGTGATGAAATTGAGTACATTGCAGTGAATTGAGAATGAAGAGAGAGAGGAAGGAGAATCTGGATTTGAAATTGAGAGAAGAGAGAGGAAAAATGTTAGAAATGAAGAAAGGAAAGGAAAAATGTTTGGTAAATTAGGTTTTGTTTTTCTCCATNGCTGRCGNTGCTACTATC
TARGET=218,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04004 Ps_contig_mira-and-tgicl-ass_franssen_31795
SEQUENCE=ACTCTTGGTTTTTCGTACAAGAATTTTAATTGGATTGTCGGATGAGATTTTCGTATGAACTTCATTTTTTCGTATTTTCCGTTAGAAGATGTTGAAACTAATCCCGTCGAAGATGATTGGAGTGCTCAATTTCAAAGCATTGATGAGCCGTGCTCTGATGCTTTTTCAGTGACACCAGTAGGATTGTGTGGTAAAACCAAAATTGAAAATCCACAATTTGGGAATGGCTTCTCTGCTGCTCCTTGTAATGAAATGTCCTCAATAAAAGAAGTGGCAGAGACTGCTGGACCAACATATGGAAACACTATTCCCAACCAGGATGCCGCTTTCTATGAAAAGAAAGCCGTGCGTCAGTACAGCCCTGTTTCTGTTTTCGAAGGCAGTAGTGGTGCTTCCTCAGTTGAGAATTCCACCTTTGACCTGCCCGTCGTCGTCCCAATAAAGCGTGCTCGAAGTAAGCGCCGGCGTCCCTCAAAGTCAAACCCCGTATTTTCATTTTCTTTCATACCTATTTCACCGGCATTGCAAAAATATTATAAAACACCTGTGAAGAAACAGAGAAAAAAGGAGCTCTACGTGATCTCAGGCGATGCTGAGACGGGATCTCAAGAGTCAGTTATCTTCCGAAAATGCACGCATTGCGAGATCACGAAGACTCCACAATGGAGAGAGGGACCTAACGGCCCCAAAACGCTATGCAATGCTTGCGGTGTTCGGTACAGGTCTGGCCGCCTCTATCCTGAATACCGTCCAGCAAACAGCCCCACTTTCGTCGAATCAATTCACTCAAACTGTCACAAAAAGGTTCTCGAGATGCGAGGCGGCGGTGTCATCAAAGGAGGTGCTAGGAGCTGTTCTACATCGCTGCCATCAACTCTTCCCGGAAATTCGGTAGGACAGCATTATCATGGAACTTATTGAAAAGACCAAACGGTATACTAGTCTCAGACATGATTCTCCTCTGTCTATTGTTATAATTTAGTCTCATGTTATATTACTTAGCATCATTTGTTTAATTAGTGTAAATTGATTGTTAAAATAAAAAAGGTAGTAGTTGCATCAGATCAGTGATTAGTGCTTTTGTTAGGCATTGAGGAAACAATGTGTTAAAAAGTTGAAGAAGTTGAGAAAGGTATGTTAATGTAGAGATGTCTAATTGGATGGATGACATGACATGGCATGGCATGGCATGGAAGTGAGGTTATATATATGTATGTATGTATGGTTGAAGTAGAGACAGGAATCCTTTGCATTACTTGCTGCATGTGCTATGAGGACATGAAGTCAAAGGATTAATTAAAGGAAAGGACAATGATATCACATGTATTGTAACATGGCACAAAGAAATTATAGTGGAAAGCACATGCATTGCTATTCCATAGGCTGGAAAAATGTCTTTCCAGATTACAGACAGCTTCAACTTGGGTTTTGTTATTTACCCTTTATAAAACAGATTATTAGAATTTGTTTTAGTTTGAACTATAGTTTGTTTCATAAGGATATTATTATCTGTATTTTTGTTGTTCATTTTAATGTCTACTTTGTACTTTATATTTTATACATTTTATATATTTTTGTTTTTGTTTTATTGAGGAAGGCATGTTTGTTACCTTTTTACATGTTAACTGTGTTTTTTTCTTTTCTTTCTATGAACAGCATGTTGAAATTTTCATGTGAATGAACAATTAGATATTAAAGAC
TARGET=1174,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3295
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.325886
PRIMER_LEFT_SEQUENCE=TAGGCATTGAGGAAACAATGTG
PRIMER_RIGHT_SEQUENCE=ACATGCAGCAAGTAATGCAAAG
PRIMER_LEFT=1084,22
PRIMER_RIGHT=1266,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.326
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.4666
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.463021
PRIMER_LEFT_1_SEQUENCE=TAGGCATTGAGGAAACAATGTG
PRIMER_RIGHT_1_SEQUENCE=TCATAGCACATGCAGCAAGTAA
PRIMER_LEFT_1=1084,22
PRIMER_RIGHT_1=1273,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=59.537
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.6264
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.622823
PRIMER_LEFT_2_SEQUENCE=TAGGCATTGAGGAAACAATGTG
PRIMER_RIGHT_2_SEQUENCE=GCAGCAAGTAATGCAAAGGATT
PRIMER_LEFT_2=1084,22
PRIMER_RIGHT_2=1262,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=60.623
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.7634
PRIMER_LEFT_3_PENALTY=0.003589
PRIMER_RIGHT_3_PENALTY=0.759842
PRIMER_LEFT_3_SEQUENCE=TAGGCATTGAGGAAACAATGTG
PRIMER_RIGHT_3_SEQUENCE=AATGCAAAGGATTCCTGTCTCT
PRIMER_LEFT_3=1084,22
PRIMER_RIGHT_3=1253,22
PRIMER_LEFT_3_TM=59.996
PRIMER_RIGHT_3_TM=59.240
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=0.9214
PRIMER_LEFT_4_PENALTY=0.595491
PRIMER_RIGHT_4_PENALTY=0.325886
PRIMER_LEFT_4_SEQUENCE=GCTTTTGTTAGGCATTGAGGAA
PRIMER_RIGHT_4_SEQUENCE=ACATGCAGCAAGTAATGCAAAG
PRIMER_LEFT_4=1076,22
PRIMER_RIGHT_4=1266,22
PRIMER_LEFT_4_TM=60.595
PRIMER_RIGHT_4_TM=60.326
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_04005 Ps_contig_mira-and-tgicl-ass_franssen_31795
SEQUENCE=ACTCTTGGTTTTTCGTACAAGAATTTTAATTGGATTGTCGGATGAGATTTTCGTATGAACTTCATTTTTTCGTATTTTCCGTTAGAAGATGTTGAAACTAATCCCGTCGAAGATGATTGGAGTGCTCAATTTCAAAGCATTGATGAGCCGTGCTCTGATGCTTTTTCAGTGACACCAGTAGGATTGTGTGGTAAAACCAAAATTGAAAATCCACAATTTGGGAATGGCTTCTCTGCTGCTCCTTGTAATGAAATGTCCTCAATAAAAGAAGTGGCAGAGACTGCTGGACCAACATATGGAAACACTATTCCCAACCAGGATGCCGCTTTCTATGAAAAGAAAGCCGTGCGTCAGTACAGCCCTGTTTCTGTTTTCGAAGGCAGTAGTGGTGCTTCCTCAGTTGAGAATTCCACCTTTGACCTGCCCGTCGTCGTCCCAATAAAGCGTGCTCGAAGTAAGCGCCGGCGTCCCTCAAAGTCAAACCCCGTATTTTCATTTTCTTTCATACCTATTTCACCGGCATTGCAAAAATATTATAAAACACCTGTGAAGAAACAGAGAAAAAAGGAGCTCTACGTGATCTCAGGCGATGCTGAGACGGGATCTCAAGAGTCAGTTATCTTCCGAAAATGCACGCATTGCGAGATCACGAAGACTCCACAATGGAGAGAGGGACCTAACGGCCCCAAAACGCTATGCAATGCTTGCGGTGTTCGGTACAGGTCTGGCCGCCTCTATCCTGAATACCGTCCAGCAAACAGCCCCACTTTCGTCGAATCAATTCACTCAAACTGTCACAAAAAGGTTCTCGAGATGCGAGGCGGCGGTGTCATCAAAGGAGGTGCTAGGAGCTGTTCTACATCGCTGCCATCAACTCTTCCCGGAAATTCGGTAGGACAGCATTATCATGGAACTTATTGAAAAGACCAAACGGTATACTAGTCTCAGACATGATTCTCCTCTGTCTATTGTTATAATTTAGTCTCATGTTATATTACTTAGCATCATTTGTTTAATTAGTGTAAATTGATTGTTAAAATAAAAAAGGTAGTAGTTGCATCAGATCAGTGATTAGTGCTTTTGTTAGGCATTGAGGAAACAATGTGTTAAAAAGTTGAAGAAGTTGAGAAAGGTATGTTAATGTAGAGATGTCTAATTGGATGGATGACATGACATGGCATGGCATGGCATGGAAGTGAGGTTATATATATGTATGTATGTATGGTTGAAGTAGAGACAGGAATCCTTTGCATTACTTGCTGCATGTGCTATGAGGACATGAAGTCAAAGGATTAATTAAAGGAAAGGACAATGATATCACATGTATTGTAACATGGCACAAAGAAATTATAGTGGAAAGCACATGCATTGCTATTCCATAGGCTGGAAAAATGTCTTTCCAGATTACAGACAGCTTCAACTTGGGTTTTGTTATTTACCCTTTATAAAACAGATTATTAGAATTTGTTTTAGTTTGAACTATAGTTTGTTTCATAAGGATATTATTATCTGTATTTTTGTTGTTCATTTTAATGTCTACTTTGTACTTTATATTTTATACATTTTATATATTTTTGTTTTTGTTTTATTGAGGAAGGCATGTTTGTTACCTTTTTACATGTTAACTGTGTTTTTTTCTTTTCTTTCTATGAACAGCATGTTGAAATTTTCATGTGAATGAACAATTAGATATTAAAGAC
TARGET=1209,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3295
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.325886
PRIMER_LEFT_SEQUENCE=TAGGCATTGAGGAAACAATGTG
PRIMER_RIGHT_SEQUENCE=ACATGCAGCAAGTAATGCAAAG
PRIMER_LEFT=1084,22
PRIMER_RIGHT=1266,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.326
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.4666
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.463021
PRIMER_LEFT_1_SEQUENCE=TAGGCATTGAGGAAACAATGTG
PRIMER_RIGHT_1_SEQUENCE=TCATAGCACATGCAGCAAGTAA
PRIMER_LEFT_1=1084,22
PRIMER_RIGHT_1=1273,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=59.537
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.6264
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.622823
PRIMER_LEFT_2_SEQUENCE=TAGGCATTGAGGAAACAATGTG
PRIMER_RIGHT_2_SEQUENCE=GCAGCAAGTAATGCAAAGGATT
PRIMER_LEFT_2=1084,22
PRIMER_RIGHT_2=1262,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=60.623
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.7634
PRIMER_LEFT_3_PENALTY=0.003589
PRIMER_RIGHT_3_PENALTY=0.759842
PRIMER_LEFT_3_SEQUENCE=TAGGCATTGAGGAAACAATGTG
PRIMER_RIGHT_3_SEQUENCE=AATGCAAAGGATTCCTGTCTCT
PRIMER_LEFT_3=1084,22
PRIMER_RIGHT_3=1253,22
PRIMER_LEFT_3_TM=59.996
PRIMER_RIGHT_3_TM=59.240
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=0.9214
PRIMER_LEFT_4_PENALTY=0.595491
PRIMER_RIGHT_4_PENALTY=0.325886
PRIMER_LEFT_4_SEQUENCE=GCTTTTGTTAGGCATTGAGGAA
PRIMER_RIGHT_4_SEQUENCE=ACATGCAGCAAGTAATGCAAAG
PRIMER_LEFT_4=1076,22
PRIMER_RIGHT_4=1266,22
PRIMER_LEFT_4_TM=60.595
PRIMER_RIGHT_4_TM=60.326
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_04006 Ps_contig_mira-and-tgicl-ass_franssen_31797
SEQUENCE=AAGTAGGTAATACAAACGCAGAGTACGCGGGAGGTCACTTTTAGACGGGATGGAGCATATTGCGATGGTAAAGGGTGACATTGGGGATGGATGTGATGTTCTTGTGAGGGTACACTCAGAGTGTCTCACTGGAGACATATTTGGATCTGCTAGATGTGACTGTGGAAATCAGCTTGCTCTTGCAATGCAACAGATTGAGGCTGCTGGTAGAGGCGTGTTAGTATATCTTCGTGGACATGAAGGAAGGGGTATTGGATTAGGCCACAAGCTTCGTGCTTATAACCTACAGGATGAGGGAAGGGATACCGTAGAAGCCAATGAGGAGTTGGGATTGCCAGTTGACTCAAGAGAGTATGGAATTGGTGCACAGATATTGAGGGACTTGGGTGTTCGTTCTATGAAGCTGATGACAAACAATCCGGCAAAATATGTTGGGCTCAAAGGTTATGGTTTGTCAATTTCCGGTAGGATCCCATTGTTGTCACTTATCACTAAAGAAAACAGGAGATATTTGGAAACCAAACGTTTGAAAATGGGGCACGCCTATGGCTTGGAATTTTACAGCAAATTGAATCATAGTGACAATGGAAATGGTAATGCCGGTAGTGTTGATGATTCCAATGCTGCCCCTGGCTCATAGACCTGCTGTTTTTTTTCTCTCTCTCTCTCTCTAAGATACGCAGCATGCAACAANARAAGAAGGGAAGTGTAAATTTCTTGCACTGATTCAGATGGATATATGAAGATAGAAGTGTCCTGCTTCTACTTGTGTATTTTTTTTTCAATTCATTTGCATGATTTGTCTTCTGTCTGGCTATAAAGGTCATTTGAAACTTCATATAGGGTCATGCGATGGGCCTAGACTAAATTTGGTAGGTTTAACTTTTCTTGCTTTTCTTTTAGTAAGAAAACTAGNGGTTC
TARGET=656,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04007 Ps_contig_mira-and-tgicl-ass_franssen_31801
SEQUENCE=GTAAAGGTTTTTGAACTATAATATTTTTATTAATCTCTTGTAGAATCTACATAAGTGTTCATTCAATGTAAAGATTACGCACAATGGCACTATGATTTTGTTGAAGCTACAAAGTGTAGTTTTTGCCAAAATCATGGAAGTTTGACATCCCAGAAAGAACCAAACTCGCCGACAATCTAGATTTGAGAATGACTATATTTCTTTATTCTCTTTTGTCAAATACGCATCGATAACCTTTAGATATAGTTCATAAAATGACAACTACCAATGGTTGTGAACTAACACGATTTCTTCCTTTAACTCAACCTATATGTGTTGTCCTTCCAAAAATGTCGTAGCAGTGTTGAAAAGCAGTATGATGTGAAGCGATTTTATCTTTCTCACAGTGAAGAACGTCTCCAAGTCGCGCCTTGCATGAGCTCAACGACCAGAGCAGATGCGCCTCCTCCCCCATTGCAGATGGCGCCAACACCATACTTTCCACCCTTATGTCTCAATACCCCTAATAATGAAACTAAGATGCGAGCTCCGCTGCAACCCAATGGATGTCCCAATGACACAGCACCTCCGTGTGCATTAACTTTTTCAGGATCAAGACTGAGAAGCTTCTGATTCGCAAGAGCCACAACTGAAAAAGCTTCGTTTATTTCATAATAATCAATTTGAGAAGCCTTCAGACCAGCATTTGATATAGCTTTAGGTATTGCAAGGGAAGGAGCTGTTGTAAATAATTCGGGTGCCTGAGCTGCATCTGCAAACCCTTTTATCTTTGCAATTATATGCAAACCAAGCTCACGTGCCTTCTCTTCGCTCACTAGCACTAATGCAGCCGCACCATCACTTATGCTAGAAGCATTGCCAGCAGTCACCGTACCCCCAACCTTTTTAAAGTTTGGTCCAAGCTTCCTTAACTTTGCAGCATCAAACTTCCCCAAACCTTCATCTTTATCTACTAGTGTGGAAGGCTTTCCTCTTCCATTGGAAATTTCAACCGGAACTATCTCCCAAGCAAAATGACCAGACTTTTGTGCAGATAATCCTCTCTCAAAGCTTTGAATGGCGTAAGCATCCTGCTGATCTCTAGTTATGGAATGCTGATCTGCACAGAGTTCTGCACAAACTCCCATGCCGAAGTCATTATAGACATCCCAAAGGCCATCTTTAAGCATACCGTCAATGATAGTATCATGTCCTAACCGAGATCCTTTCCTTGCTTGGACAATGTACTTAGGTGCATTTGACATGCTTTCCATACCACCAACCACAACAACATCATTGGAACCCAACTGAATTGTTTGTGCAGCAAGCATGGTAGCTTTCATCCCAGATGAACACACTTTGTTAATAGTAGTGCAGACAACAGATGTAGGTATTCCAGCAGCTAATGCAGCCTGTCTAGCAGGAGCCTGGCCTAAATTAGCACTAAGAACATTCCCAAAAAAGACTTCTTGCACAAGGGATGGATCAACATTGGCTCTCTTGAGAGCCGCTTGAATAGCGAGTGAGCCTAGGTGTGTAGCTGAAAGAGATGATAAGGAACCAAGGAAACCACCCATTGGTGTCCTTGCAACACCAACAATACAAACATCTCGGGATTTTGAAGACATGGATGGATGGATGGGGAGCGTGGTCGGAAAAGAGTTTGATTTGATGAGAGATGAAATTCAAAGAAAGGGAAAGTNAGAGAGNANNYCGACAAGACCAGAAGGCTGGAAAGTTAAATACGTTAATGATATTGTCAAGGGACCCACAACCACACCTACTTGAATTGTCTTGTCTTTTGCATGCTATTCT
TARGET=1606,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04008 Ps_contig_mira-and-tgicl-ass_franssen_31804
SEQUENCE=GATATCATTAGTTAATAAAAATAAAAACTTAAAATAGAGTGCGATATAACCAATTGCCCCTTTTCCAATTCAGTTGCAGCAATTTTCTTCACCCATGGCTTACGTCGAACGAGGTGTTGTCAAATCAAAGCGATCGATATGGCGAATCAAGACAATCACTGATTTTTTCTGGGCCATTGTTAACTTCATAGGCGTGTTTTTCACTACGATGTTCTCGATGGAAAAGTCAGATGCCTACAAGAAAGGGTCTGCCGGTAAGAAATGGTCCGGTGGCGGTGGTGGTGGTGGCGGCGGCGGTGGTGGTGGTCCTCGCGGCCCTCCTCGTGGTGGCCTCGACAATGTCCGTGGGCTAGATAGTGTCCGCGGTGATCATAGTTCACTGCCTGCATGCGGCTCATGCTGTGGTTGAGTGTGTTAGAGATCTCATCTGTCTATATTTGTCACAGTTTGTATCTGATTTTCGACTGACGTAATATGCCTATATCATATACGATTTTCAAGCTATGTGTGCAAATAGGTTGAAACAGCTAAAAACTTGCATAATAATACTTGTCTTGCTTGGGTATTGAATTAAAAATCCTAACAAATTATTCNANATTATCATGCTTTATAG
TARGET=275,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0911
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.086698
PRIMER_LEFT_SEQUENCE=ACGATGTTCTCGATGGAAAAGT
PRIMER_RIGHT_SEQUENCE=ACACTATCTAGCCCACGGACAT
PRIMER_LEFT=205,22
PRIMER_RIGHT=359,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.1020
PRIMER_LEFT_1_PENALTY=0.015253
PRIMER_RIGHT_1_PENALTY=0.086698
PRIMER_LEFT_1_SEQUENCE=GAACGAGGTGTTGTCAAATCAA
PRIMER_RIGHT_1_SEQUENCE=ACACTATCTAGCCCACGGACAT
PRIMER_LEFT_1=106,22
PRIMER_RIGHT_1=359,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=254
PRIMER_PAIR_PENALTY_2=0.1202
PRIMER_LEFT_2_PENALTY=0.033470
PRIMER_RIGHT_2_PENALTY=0.086698
PRIMER_LEFT_2_SEQUENCE=TATGGCGAATCAAGACAATCAC
PRIMER_RIGHT_2_SEQUENCE=ACACTATCTAGCCCACGGACAT
PRIMER_LEFT_2=137,22
PRIMER_RIGHT_2=359,22
PRIMER_LEFT_2_TM=59.967
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.1659
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.161579
PRIMER_LEFT_3_SEQUENCE=ACGATGTTCTCGATGGAAAAGT
PRIMER_RIGHT_3_SEQUENCE=CATGCAGGCAGTGAACTATGAT
PRIMER_LEFT_3=205,22
PRIMER_RIGHT_3=389,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=60.162
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=0.1768
PRIMER_LEFT_4_PENALTY=0.015253
PRIMER_RIGHT_4_PENALTY=0.161579
PRIMER_LEFT_4_SEQUENCE=GAACGAGGTGTTGTCAAATCAA
PRIMER_RIGHT_4_SEQUENCE=CATGCAGGCAGTGAACTATGAT
PRIMER_LEFT_4=106,22
PRIMER_RIGHT_4=389,22
PRIMER_LEFT_4_TM=60.015
PRIMER_RIGHT_4_TM=60.162
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_04009 Ps_contig_mira-and-tgicl-ass_franssen_31804
SEQUENCE=GATATCATTAGTTAATAAAAATAAAAACTTAAAATAGAGTGCGATATAACCAATTGCCCCTTTTCCAATTCAGTTGCAGCAATTTTCTTCACCCATGGCTTACGTCGAACGAGGTGTTGTCAAATCAAAGCGATCGATATGGCGAATCAAGACAATCACTGATTTTTTCTGGGCCATTGTTAACTTCATAGGCGTGTTTTTCACTACGATGTTCTCGATGGAAAAGTCAGATGCCTACAAGAAAGGGTCTGCCGGTAAGAAATGGTCCGGTGGCGGTGGTGGTGGTGGCGGCGGCGGTGGTGGTGGTCCTCGCGGCCCTCCTCGTGGTGGCCTCGACAATGTCCGTGGGCTAGATAGTGTCCGCGGTGATCATAGTTCACTGCCTGCATGCGGCTCATGCTGTGGTTGAGTGTGTTAGAGATCTCATCTGTCTATATTTGTCACAGTTTGTATCTGATTTTCGACTGACGTAATATGCCTATATCATATACGATTTTCAAGCTATGTGTGCAAATAGGTTGAAACAGCTAAAAACTTGCATAATAATACTTGTCTTGCTTGGGTATTGAATTAAAAATCCTAACAAATTATTCNANATTATCATGCTTTATAG
TARGET=296,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0911
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.086698
PRIMER_LEFT_SEQUENCE=ACGATGTTCTCGATGGAAAAGT
PRIMER_RIGHT_SEQUENCE=ACACTATCTAGCCCACGGACAT
PRIMER_LEFT=205,22
PRIMER_RIGHT=359,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.1020
PRIMER_LEFT_1_PENALTY=0.015253
PRIMER_RIGHT_1_PENALTY=0.086698
PRIMER_LEFT_1_SEQUENCE=GAACGAGGTGTTGTCAAATCAA
PRIMER_RIGHT_1_SEQUENCE=ACACTATCTAGCCCACGGACAT
PRIMER_LEFT_1=106,22
PRIMER_RIGHT_1=359,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=254
PRIMER_PAIR_PENALTY_2=0.1202
PRIMER_LEFT_2_PENALTY=0.033470
PRIMER_RIGHT_2_PENALTY=0.086698
PRIMER_LEFT_2_SEQUENCE=TATGGCGAATCAAGACAATCAC
PRIMER_RIGHT_2_SEQUENCE=ACACTATCTAGCCCACGGACAT
PRIMER_LEFT_2=137,22
PRIMER_RIGHT_2=359,22
PRIMER_LEFT_2_TM=59.967
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.1659
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.161579
PRIMER_LEFT_3_SEQUENCE=ACGATGTTCTCGATGGAAAAGT
PRIMER_RIGHT_3_SEQUENCE=CATGCAGGCAGTGAACTATGAT
PRIMER_LEFT_3=205,22
PRIMER_RIGHT_3=389,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=60.162
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=0.1768
PRIMER_LEFT_4_PENALTY=0.015253
PRIMER_RIGHT_4_PENALTY=0.161579
PRIMER_LEFT_4_SEQUENCE=GAACGAGGTGTTGTCAAATCAA
PRIMER_RIGHT_4_SEQUENCE=CATGCAGGCAGTGAACTATGAT
PRIMER_LEFT_4=106,22
PRIMER_RIGHT_4=389,22
PRIMER_LEFT_4_TM=60.015
PRIMER_RIGHT_4_TM=60.162
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_04010 Ps_contig_mira-and-tgicl-ass_franssen_31815
SEQUENCE=AAAAACTTTTAAGTAATCACTCTAAATAAGATTATTAAAACCCAAGATTCACAACCGTACCATTTATCATAAAAGATTATAGTCACAACCATCATGTACTACTAATATGATACAAATCAGACAAACCCAATTATTTGAGAACCCTACTAGGGAATTTTTCTTCTCTTATTATTTTGAAGTTCCCTTTTCTTATGACTAATATCTTACATTATTCCTTCCATGCAAAATCTACAAAATATGTTGCTTGAAATTGACCTCTTCATTTCTCATTGACTCCAAATTTCTTTTCACTCCCATCTTTGCCAATAACAATCCCAACCAATTTACAAGGTGCAGCATCCTCGAGCTGTTGAACCACATTTCTCATACTTGGTCTCATAGCAGGAAGAGTTGCAGTACAAAGAACTGCAGTTCTCAAAACCTTACAACCTTCCTCTTTATACATCTCCGGAATTCGCGAATCCACCACACTCATAAACTTTTCCTTGCTCCTCGTCTTCCCATGAACCCAACTAACTATATCCTTGTTTTCTCCAAACTCTGTCTCACTCGGCCTTTTACCTGTTACTAGCTCCATCAACACGACTCCGAAACTATATACATCACTTTTCTCATTCACTCTGTATGTGTAACCATATTCGGGTGCGATGTAGCCGTGAGTTCCGGCGATGATATGAGTAGAATCCTTAACTACATTGGCATGAACAATCTTAGCAAGACCAAAATCAGCAATCCTAGGTTTCAAAAACTCATCCAACAAAATGTTACTAGACTTAACATCTCTATGAATCACAGGTCTTTCACACCCATGATGCAAATACTCCAACCCTTTAGCAGCACCAACCGCAATTTCATATCTTGTTTCCCAATCAAGCTCCATCTTCCCATTAGTATGAAGCCTATCCCACAAACTTCCATTCGGCAAATACTCATACACCAATAAACTCGAGTCCTCGCTCGTTATACTGCAGTAAAGTTTCACCACATTGACGTGTCTTATTGAACTCAACGACTGAACCTCCGCGTCGAATTCCCTCGACTTAGAACCACCACCACCTCCGCGCTTCGCCAGCATCGGAGTACTACTCCACGACTTTTTCCGGGAACCATAATCAGTGTTCCATATATGCTTAACCGCGAGATCTTTTCCATTTGCAAGTGTGACTCTATAAACATTCCCTGAACCTTCCTTTTTCCTATTAGATTCTCTTGTCTTTATAAGAATCAAAGAAT
TARGET=1046,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2364
PRIMER_LEFT_PENALTY=0.062823
PRIMER_RIGHT_PENALTY=0.173582
PRIMER_LEFT_SEQUENCE=GTTTCACCACATTGACGTGTCT
PRIMER_RIGHT_SEQUENCE=GAAAAAGGAAGGTTCAGGGAAT
PRIMER_LEFT=975,22
PRIMER_RIGHT=1195,22
PRIMER_LEFT_TM=59.937
PRIMER_RIGHT_TM=59.826
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.2780
PRIMER_LEFT_1_PENALTY=0.062823
PRIMER_RIGHT_1_PENALTY=0.215198
PRIMER_LEFT_1_SEQUENCE=GTTTCACCACATTGACGTGTCT
PRIMER_RIGHT_1_SEQUENCE=AAGGAAGGTTCAGGGAATGTTT
PRIMER_LEFT_1=975,22
PRIMER_RIGHT_1=1191,22
PRIMER_LEFT_1_TM=59.937
PRIMER_RIGHT_1_TM=60.215
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.2780
PRIMER_LEFT_2_PENALTY=0.062823
PRIMER_RIGHT_2_PENALTY=0.215198
PRIMER_LEFT_2_SEQUENCE=GTTTCACCACATTGACGTGTCT
PRIMER_RIGHT_2_SEQUENCE=AAAAGGAAGGTTCAGGGAATGT
PRIMER_LEFT_2=975,22
PRIMER_RIGHT_2=1193,22
PRIMER_LEFT_2_TM=59.937
PRIMER_RIGHT_2_TM=60.215
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.2780
PRIMER_LEFT_3_PENALTY=0.062823
PRIMER_RIGHT_3_PENALTY=0.215198
PRIMER_LEFT_3_SEQUENCE=GTTTCACCACATTGACGTGTCT
PRIMER_RIGHT_3_SEQUENCE=AAAGGAAGGTTCAGGGAATGTT
PRIMER_LEFT_3=975,22
PRIMER_RIGHT_3=1192,22
PRIMER_LEFT_3_TM=59.937
PRIMER_RIGHT_3_TM=60.215
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.3203
PRIMER_LEFT_4_PENALTY=0.146683
PRIMER_RIGHT_4_PENALTY=0.173582
PRIMER_LEFT_4_SEQUENCE=CCATTCGGCAAATACTCATACA
PRIMER_RIGHT_4_SEQUENCE=GAAAAAGGAAGGTTCAGGGAAT
PRIMER_LEFT_4=914,22
PRIMER_RIGHT_4=1195,22
PRIMER_LEFT_4_TM=59.853
PRIMER_RIGHT_4_TM=59.826
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_04011 Ps_contig_mira-and-tgicl-ass_franssen_31816
SEQUENCE=TTCGGTGGTGAAATTGATGTAAACCCTAGATCGTGATTCACGATTAGAGAGATATCAGATATTCTGGTAAAAGGAAAACGAAAATGGAAGGGAAGGGAAGTAAGCAAATGAAGGTGTTTCTTTAATCATATGGAAATGGAATTATGTC
TARGET=86,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04012 Ps_contig_mira-and-tgicl-ass_franssen_31833
SEQUENCE=ACTTAAGAACAGAACCTAGTTTTACAAATCGARGGATAACATAGGGTGAGAGAACAAAACACAAACAAACAAACATACATACTCTGTTTTTCTAATGGACTCACTCTCTCTCTTCTGCACCGGAGCTCTTCTCGCCGGCGGCCTATATTGGTTCGTCTGTATCCTCGGCCCCGCCGAACGAAAAGGCAAGCGCGCCACCGATCTATCCGGTGGTTCCATTTCCGCCGAGAAAGTCCAAGACAGCTACAAACAATACTGGTCCTTCTTCCGTCGTCCAAAAGAGATCGAAACCGCAGAGAAAGTCCCAGATTTCGTTGATACCTTTTACAATTTAGTTACAGATATTTACGAATGGGGTTGGGGTCAATCCTTCCATTTCTCACCTTCCCTCCCTGGTAAATCCCACCGTGACGCCACGCGCCTCCACGAAGAAATGGCTGTGGATCTCATCGAGGTGAAATCCGGAGACAAAATCCTCGACGTAGGATGCGGTGTTGGTGGTCCCATGAGAGCCATCGCGTCTCACTCACGCGCTAACGTCGTTGGAATCACGATCAACGAGTATCAAGTGAAGCGAGCGAAGATACACAACAAAAAAGCCGGGCTCGATTCTCTATGCGACGTCGTTTGCGGTAATTTCCTCGAGATGCCGTTTCCCGATAACACTTTCGACGGCGCGTACTCAATCGAAGCGACGTGCCACGCGCCTAAGCTTGAAGAAGTTTACGCTGAAATTTTTAGGGTTTTGAAACCCGGCGCTCTTTACGTTTCATATGAATGGGTCACAACAGAGAAATTCATATCTGAAAATTCAGAACATGTTGAGATTATTCAAGGAATTGAAAGAGGGGATGCTTTGCCTGGGCTTAGAAGCTACGCTGATATAGCTGAAACAGCTAAGAAAGTTGGGTTTGAGGTTGTTAAGGAGCGGGATCTGGCTCAGCCACCGGCTGAGCCGTGGTGGAGCCGTTTGAAAATGGGTCGGCTTGCTTATTGGCGTAACCATGTTGTGGTAACTGTTCTGTCTGCATTGGGAATAGCGCCCAAAGGGACGGTTGATGTTCATGAGATGCTGTTCAAGACTGCTGACTATTTGACCAGAGGGGGTGATACTGGAATTTTCTCTCCTATGCACATGATTCTCTGCAGAAAACCAAAAGAACAAGAAAAGTGAATTTCTGGTGGTGGTGATCAAGGTATCTGGATTTAAAATGAATTTGTTTTTTTTAAAATAATTTTATTTTAAAGTAAGTTTAACTTATTGTTTCTTTGAACTGGATGTAATATGTTTTTTTTTTCTATTTTTTGTCTAATTGTTAGTTTTTGTTTTAATATTTCTTTATTGTCCTTTTGGGTTTTATTTTAGTTCTTCTAGTTACTTGTTACTACTGTTACTTGTTAGTTGTATTTCATTTAGTGGTAATTTAATTAGTTTAAAGTT
TARGET=61,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04013 Ps_contig_mira-and-tgicl-ass_franssen_31833
SEQUENCE=ACTTAAGAACAGAACCTAGTTTTACAAATCGARGGATAACATAGGGTGAGAGAACAAAACACAAACAAACAAACATACATACTCTGTTTTTCTAATGGACTCACTCTCTCTCTTCTGCACCGGAGCTCTTCTCGCCGGCGGCCTATATTGGTTCGTCTGTATCCTCGGCCCCGCCGAACGAAAAGGCAAGCGCGCCACCGATCTATCCGGTGGTTCCATTTCCGCCGAGAAAGTCCAAGACAGCTACAAACAATACTGGTCCTTCTTCCGTCGTCCAAAAGAGATCGAAACCGCAGAGAAAGTCCCAGATTTCGTTGATACCTTTTACAATTTAGTTACAGATATTTACGAATGGGGTTGGGGTCAATCCTTCCATTTCTCACCTTCCCTCCCTGGTAAATCCCACCGTGACGCCACGCGCCTCCACGAAGAAATGGCTGTGGATCTCATCGAGGTGAAATCCGGAGACAAAATCCTCGACGTAGGATGCGGTGTTGGTGGTCCCATGAGAGCCATCGCGTCTCACTCACGCGCTAACGTCGTTGGAATCACGATCAACGAGTATCAAGTGAAGCGAGCGAAGATACACAACAAAAAAGCCGGGCTCGATTCTCTATGCGACGTCGTTTGCGGTAATTTCCTCGAGATGCCGTTTCCCGATAACACTTTCGACGGCGCGTACTCAATCGAAGCGACGTGCCACGCGCCTAAGCTTGAAGAAGTTTACGCTGAAATTTTTAGGGTTTTGAAACCCGGCGCTCTTTACGTTTCATATGAATGGGTCACAACAGAGAAATTCATATCTGAAAATTCAGAACATGTTGAGATTATTCAAGGAATTGAAAGAGGGGATGCTTTGCCTGGGCTTAGAAGCTACGCTGATATAGCTGAAACAGCTAAGAAAGTTGGGTTTGAGGTTGTTAAGGAGCGGGATCTGGCTCAGCCACCGGCTGAGCCGTGGTGGAGCCGTTTGAAAATGGGTCGGCTTGCTTATTGGCGTAACCATGTTGTGGTAACTGTTCTGTCTGCATTGGGAATAGCGCCCAAAGGGACGGTTGATGTTCATGAGATGCTGTTCAAGACTGCTGACTATTTGACCAGAGGGGGTGATACTGGAATTTTCTCTCCTATGCACATGATTCTCTGCAGAAAACCAAAAGAACAAGAAAAGTGAATTTCTGGTGGTGGTGATCAAGGTATCTGGATTTAAAATGAATTTGTTTTTTTTAAAATAATTTTATTTTAAAGTAAGTTTAACTTATTGTTTCTTTGAACTGGATGTAATATGTTTTTTTTTTCTATTTTTTGTCTAATTGTTAGTTTTTGTTTTAATATTTCTTTATTGTCCTTTTGGGTTTTATTTTAGTTCTTCTAGTTACTTGTTACTACTGTTACTTGTTAGTTGTATTTCATTTAGTGGTAATTTAATTAGTTTAAAGTT
TARGET=104,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04014 Ps_contig_mira-and-tgicl-ass_franssen_31836
SEQUENCE=CTACCCTAAAAAATTTTAGGGTAATAATTATACAAGACAAACTTTCCCTTGGAGGGAATATTCCAGTATCAATTGGAAAGTTATATAATCTAAAAAAGTTACAATGTATCGACCAATTTTCTGGTCGGACCAATACCTTCTGATGGTGTCCTAGCCCACTTTACAGAAAGCTCGTTTGTTGGAAACCGAGGTTTGTGTGGAGTACAAATTGATTCAACATGTAAAGATGATGGTTCGCCTGGGGAGAAATCTTCAGACCAAAATCAAAATGAAAAGAAGAAATATTCTGGCCGACTTCTCATCAGTGCATCAGCAACTGTGGGTGCACTACTTTTGGTGGCACTTATGTGCTTCTGGGGTTGTTTTCTTTATAAAAAGTTTGGTAAAAATGATAGAGTTAGTCTTGCAGTGGATGTTGGTCCAGGTGCATCGATCGTGATGTTTCATGGAGACCTACCATATTCTTCGAAAGACGTTATTAAGAAACTAGAGACTTTGAATGAGGAACACATAATAGGAGTAGGGGGATTTGGAACTGTTTACAAGCTCGCAATGGATGATGGGAATGTATTTGCATTGAAAAGGATTGTAAAGATGAATGAGGGCTTTGATCGATTTTTTGAGAGGGAGCTTGCAATTCTTGGTAGCATCAAACACCGTTATTTAGTGAACTTACGTGGATATTGCAACTCACCTACCTCAAAGTTGTTAATATATGACTACCTACCTGGTGGTAGTCTTGATGAAGTTCTTCATGAAAGATCCGAGCAACTAGACTGGGATTCACGGTTGAATATAATCATGGGAGCTGCAAAAGGGCTTGCGTACTTACACCATGATTGTTCACCTAGGATCATACACCGAGACATAAAGTCAAGCAACATTTTGCTCGATGGAAAATTGGATGCTCGGGTTTCTGATTTTGGACTTGCCAAACTTCTGGAAGATGAAGAATCTCACATCACTACTATTGTTGCTGGAACATTCGGTTATCTAGCTCCAGAGTATATGCAAAGTGGCAGAGCAACCGAGAAGACGGATGTTTATAGTTTTGGGGTGTTGACACTTGAAGTGCTTAGTGGAAAGCGGCCAACAGACGCATCATTCATTGAAAAGGGTCTCAATATTGTTGGTTGGTTGAATTTCCTTATTACCGAAAATAGGCCGAGGGAAATTGTGGATCTACTATGTGACGGGGTGCAGGTGGAGAGCCTTGATGCCTTGCTCTCTGTGGCCATTCAGTGTGTTTCCTCGAGTCCAGAGGATCGACCCACTATGCACCGAGTGGTCCAGTTGCTCGAATCGGAGGTCGTAACTCCATGCCCAAGTGACTTCTATGACTCGGACTAGAAAGGCTGCGAAGACAAATTATGTTTGCATCCATGTCGTTGCAAATTACTTGAGTCAGATGTGATGAAACAGGTTAAGAATCTGCGTGCAACTTTTCATATGTGCATTCATTCAAAGTGTAAGTTTATGACGGAAGATGTCCTTCATTACAGCACTTCACAACAGAAGGTGCTGTTATTCAACGGATATCATCATTCAACATTTTCATGCATTTCTTTGCCTTCCTGATGGAGTTGCATTGTTATATTTGATTGCACGTTCCCCTCNTTGTTCTTTTACCACTACCTTTGGTANCTATTTGAAACTCGAACG
TARGET=1129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7594
PRIMER_LEFT_PENALTY=0.241544
PRIMER_RIGHT_PENALTY=0.517877
PRIMER_LEFT_SEQUENCE=GCCAAACTTCTGGAAGATGAAG
PRIMER_RIGHT_SEQUENCE=AGTAGATCCACAATTTCCCTCG
PRIMER_LEFT=930,22
PRIMER_RIGHT=1186,22
PRIMER_LEFT_TM=60.242
PRIMER_RIGHT_TM=59.482
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.7748
PRIMER_LEFT_1_PENALTY=0.681732
PRIMER_RIGHT_1_PENALTY=0.093026
PRIMER_LEFT_1_SEQUENCE=GCATCATTCATTGAAAAGGGTC
PRIMER_RIGHT_1_SEQUENCE=CAGCCTTTCTAGTCCGAGTCAT
PRIMER_LEFT_1=1098,22
PRIMER_RIGHT_1=1357,22
PRIMER_LEFT_1_TM=60.682
PRIMER_RIGHT_1_TM=59.907
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=260
PRIMER_PAIR_PENALTY_2=0.8106
PRIMER_LEFT_2_PENALTY=0.292684
PRIMER_RIGHT_2_PENALTY=0.517877
PRIMER_LEFT_2_SEQUENCE=GTATATGCAAAGTGGCAGAGCA
PRIMER_RIGHT_2_SEQUENCE=AGTAGATCCACAATTTCCCTCG
PRIMER_LEFT_2=1004,22
PRIMER_RIGHT_2=1186,22
PRIMER_LEFT_2_TM=60.293
PRIMER_RIGHT_2_TM=59.482
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=0.8379
PRIMER_LEFT_3_PENALTY=0.681732
PRIMER_RIGHT_3_PENALTY=0.156135
PRIMER_LEFT_3_SEQUENCE=GCATCATTCATTGAAAAGGGTC
PRIMER_RIGHT_3_SEQUENCE=ACATAATTTGTCTTCGCAGCCT
PRIMER_LEFT_3=1098,22
PRIMER_RIGHT_3=1373,22
PRIMER_LEFT_3_TM=60.682
PRIMER_RIGHT_3_TM=60.156
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.8715
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=0.629991
PRIMER_LEFT_4_SEQUENCE=GCCAAACTTCTGGAAGATGAAG
PRIMER_RIGHT_4_SEQUENCE=CACCCCGTCACATAGTAGATCC
PRIMER_LEFT_4=930,22
PRIMER_RIGHT_4=1199,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=60.630
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_04015 Ps_contig_mira-and-tgicl-ass_franssen_31838
SEQUENCE=TCGGTTCTTCCCCATTTTCTTCTTGTTCTATATCCAACCATCGCTGAAATAGGATTTTCACGATACCATACATGATTTTCTCCATTCTTCAATAATTCATTGATTAATGGATCCCAATTCTCAATCATCAGGAAGTAATCCTCCCCCAAAACCTTGGGAACGAGCTGGTTCTTCGTCTGGCCCCGGGGCGCCTTTTAAACCGCCATCAGGGGGAAGCACAAGTGATGTTGTTGAGGCTTCTGGGACTGCAAAGCCTGGGGAGATTGTTACCAGTACTGACAGAAATGCAGCTATCAACAGGAGTACTCTCGCCAGACCGGTTCCCGCGAGGCCGTGGGAGCAGAATTACGGAAATACAAGCTACGGAGGTGGTGCGTTAGGCGGATATGGGTCTTCGATGAATTATAATTCTGGATATGGTTCTGGAGGAATGTATGGATCTTCTTATGGTGGCGGGTTAGGAGGAGTTGGAGGAATGTATGGTGGCGGAATGTATGGGAACAGTATGTACAGAGGAGGAGGAGGATATGGCGGAGGCCTTTATGGGTCATCTGGGATGTATGGAGGAGGTGGAATGTATAACAGTGGCCTTGGGGGTCAATGGGTGGTTATGGCATGGGAGTTGGTCCTTATGGCGGTGATCAAGATCCCAATAATCCATTTAATGAACCTCCATCCCCGCCAGGATTTTGGATTTCTATGCTGCGTGTGATGCAAGGTGTGGTTAATTTTTTTGGCCGGATATCAATACTCATAGACCAGAACACACAGGCTTTCCATTTGTTCATGACCGCAATGCTCCAGCTGTTTGATCGCTCCGGCATGTTGTACGGGGAGCTAGCTAGATTTGTGTTGCGATTGCTCGGGGTCAAAACTAAGCCCAAGAAGGTTAACCACCCGCCAGGTCCGAATGGACATCCATTTCACGGACAACCAAATTCTTCTGGAAATGTGAACTACATTGAGGGAGCAAAGACTGCTCCAAGTGGTTCTTGGGACAATGTCTGGGGAAATGACACCAGTGAATGATTTGACGAATTTAATAAGTGTGTTCAAGTACATTAAAGAAAAGAGAGAAATATCAGCATTTCAAATGAAGATACTGAAGCTGCAGAATTCATGTATATCCTAGATTTAATTGCAGTTTTCTGTTTTTCTTTGCCAATATTTGTTCTTGGTTTTACTAGAAGGTGGATCTTCTGATGATGAGATATTATCCCTGCCTTTATACCTTGTAATTGTTTAGTTTGAATATGCAGGACGCAGCTATTTCTAAGTACATTGTGTCTAGAATGGATGTAATCTTGCATGCCTGCCTATGTTTTTTTGGACCTTAAATTCCATTTCTTTAAATGTNNNACTTTCTTATATGC
TARGET=513,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0957
PRIMER_LEFT_PENALTY=0.021195
PRIMER_RIGHT_PENALTY=0.074466
PRIMER_LEFT_SEQUENCE=GGAAATACAAGCTACGGAGGTG
PRIMER_RIGHT_SEQUENCE=ACATCCCAGATGACCCATAAAG
PRIMER_LEFT=349,22
PRIMER_RIGHT=559,22
PRIMER_LEFT_TM=60.021
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=0.1319
PRIMER_LEFT_1_PENALTY=0.057411
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=CCAGTACTGACAGAAATGCAGC
PRIMER_RIGHT_1_SEQUENCE=ACATCCCAGATGACCCATAAAG
PRIMER_LEFT_1=269,22
PRIMER_RIGHT_1=559,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=291
PRIMER_PAIR_PENALTY_2=0.1327
PRIMER_LEFT_2_PENALTY=0.074466
PRIMER_RIGHT_2_PENALTY=0.058242
PRIMER_LEFT_2_SEQUENCE=ATGGTTCTGGAGGAATGTATGG
PRIMER_RIGHT_2_SEQUENCE=GCAGCATAGAAATCCAAAATCC
PRIMER_LEFT_2=416,22
PRIMER_RIGHT_2=705,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.942
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.1422
PRIMER_LEFT_3_PENALTY=0.102276
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=GCGGAATGTATGGGAACAGTAT
PRIMER_RIGHT_3_SEQUENCE=AAATGGAAAGCCTGTGTGTTCT
PRIMER_LEFT_3=485,22
PRIMER_RIGHT_3=781,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.1605
PRIMER_LEFT_4_PENALTY=0.102276
PRIMER_RIGHT_4_PENALTY=0.058242
PRIMER_LEFT_4_SEQUENCE=GCGGAATGTATGGGAACAGTAT
PRIMER_RIGHT_4_SEQUENCE=GCAGCATAGAAATCCAAAATCC
PRIMER_LEFT_4=485,22
PRIMER_RIGHT_4=705,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=59.942
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_04016 Ps_contig_mira-and-tgicl-ass_franssen_31839
SEQUENCE=ACTTGATGAGGTGAAAAGAAAGATTCACATGAGAGAAGATATGGATCTACGAATAATAGCCTCGTAGACATCGCATCGGGAAATACGTCCTTGTAGTTGTTGTTGTGCTACCTCGCTCCACAATTCCAACTCAAACCAAAAAGCCACTTCACACTCCACTCTCATGCTAGTTTCTAACTGTAACTGTCGCTATTCTAGCTGTTGGAGCTTCTAACTCAGATCTGTTTCTTATTTCTTCAATTTCAATTATCTGTAATTGGATTGGCGTAGATGGAGGGGAACTCCGGCGGTGGTGGTAGCGGTGGCGGCGGCGGTGGTAGTGGTGGTGGAGGCGGTGGCGGTGGAGGTGCTGACGTGGAGCTTCTTTGCAAAACGTTGCAGGTTGAGCATAAGCTGTTTTACTTCGATCTGAAAGAGAATCCTCGTGGTCGTTACCTTAAGATTTCTGAGAAAACCTCTGCTACTAGGTCCACCATCATCGTTCCGTTTTCCGGCATTTCGTGGTTTCTCGATCTCTTCAATTATTACGTTAATCCTGATGATCAAGAGCTTTTCAGCAAGGAATTGCAGCTCGATACCAAGGTTTTCTACTTCGACATTGGGGAGAATAGGAGAGGCCGTTTCTTAAAGGTGTCTGAAGCTTCTGTCAGCAGAAATCGGAGCACTATAATCGTTCCTGCAGGAAGTTCCAGGGATGAAGGGTGGGCAGCTTTCAGGAACATTTTGGCAGAGATTAATGAAGCCTCAAGGCTTTTCCTTCTGCCCAATCAGCAAAGTTCTGAATCCTCAGAGCGGTTAGTTGGACTTTCGGATGATGTTGGTGCTGGCTTCATATCTGGTCATAGTACTCAACCTGCCACCTCTTCTGAATTGAATGTGGACAGGTCTGTTGATTTACCAGCTCAGGAAGAAACTGGGAACTTGGGGGTCTCAAAAGTGATCAGGGCTGATCAAAAAAGATTTTTCTTTGATCTTGGGAACAACAATAGGGGCCATTTTCTCAGAATATCTGAGGTCGCAGGTTCTGATCGATCCTCCATCATTCTTCCACTGTCAGGGCTCAAGCAGTTTCATGAAATTGTCGGTCACTTTGTGGAAATCACCAAGGACCGAATTGAGGGTATGTCAGTTGCTAATGTCCGCACGATTGATCCCCCTCAAAGATGAATGCTAACTTGGGGGTTGTGGCGACCATAAGTAGTCCCCCTCTGCCAAGGTTTCGTCATGGATATATGAATTGGTGTTGTCTTTGAATCTGATTTGTGCTATTTTATTTTATTTTCCGTAGCCTCTTCTTATTTATAATATTGTTCTACAATTAATGCTTTTGAAATAAACCAAACACTTGTTGGTCAGCAGAATAATGAGAATATCTCTACAAGTTTAATTTGAATAGGTAATAACGTGAGAAAAACAGACGGGAGACTTGTACTTGATTGGATAGTTTGGCAATGGGGCTATACTTTGTAATAATGATTGTTTATTTTGCTTTCCTGCACATATTTCATATGGATTTAAGTTCTCAAA
TARGET=1267,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1873
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.153161
PRIMER_LEFT_SEQUENCE=CCCCTCAAAGATGAATGCTAAC
PRIMER_RIGHT_SEQUENCE=GTCTCCCGTCTGTTTTTCTCAC
PRIMER_LEFT=1153,22
PRIMER_RIGHT=1425,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.153
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=273
PRIMER_PAIR_PENALTY_1=0.2452
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.211105
PRIMER_LEFT_1_SEQUENCE=CCCCTCAAAGATGAATGCTAAC
PRIMER_RIGHT_1_SEQUENCE=CAAGTCTCCCGTCTGTTTTTCT
PRIMER_LEFT_1=1153,22
PRIMER_RIGHT_1=1428,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.789
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.3151
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.281044
PRIMER_LEFT_2_SEQUENCE=CCCCTCAAAGATGAATGCTAAC
PRIMER_RIGHT_2_SEQUENCE=AGTCTCCCGTCTGTTTTTCTCA
PRIMER_LEFT_2=1153,22
PRIMER_RIGHT_2=1426,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=60.281
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=0.3739
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.339815
PRIMER_LEFT_3_SEQUENCE=CCCCTCAAAGATGAATGCTAAC
PRIMER_RIGHT_3_SEQUENCE=ACAAGTCTCCCGTCTGTTTTTC
PRIMER_LEFT_3=1153,22
PRIMER_RIGHT_3=1429,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.660
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.4558
PRIMER_LEFT_4_PENALTY=0.302618
PRIMER_RIGHT_4_PENALTY=0.153161
PRIMER_LEFT_4_SEQUENCE=GGTTGTGGCGACCATAAGTAGT
PRIMER_RIGHT_4_SEQUENCE=GTCTCCCGTCTGTTTTTCTCAC
PRIMER_LEFT_4=1180,22
PRIMER_RIGHT_4=1425,22
PRIMER_LEFT_4_TM=60.303
PRIMER_RIGHT_4_TM=60.153
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_04017 Ps_contig_mira-and-tgicl-ass_franssen_31842
SEQUENCE=GCTGAGAGACCTAGTTTGTAAACATCAAAGGAAAATRGGAAATCTGATCCCAATTACACCAAATCATGGTACGATAGAGGTGCTAAAATTTTTCAGGCCAGCAAGTATAGGAAAGGCGCATGTGAGAATTGTGGAGCAATGACACATGCTGCCAAGGCATGCATGGAAAGACCTCGAAAAGTAGGAGCAAAACATACAAACAAACACATTGCTGCTGATGAAAAGGTGGAAACTTTTGAGCTTGACTATGATGGCAAACGAGATAGATGGAATGGGTATGATGCATCAACCTACGCCCGTGTGATTGAGAGATATGAAGCCAGAGATGATGCTCGAAAGAAGTACTTGAAGGAACAACAGCTGGAGAAATTGGAGAAGGGCGACCAAAATGATGAGGGCGCTGCTATTGATGAGGCTGAAGACGAAGATGATTTGAGGGTAGATGAGGCCAAGGTTGATGAAAGCAAACAAATGGACTTTGCCAAGGTTGAGAAGCGTGTGCGAACAACAGGTGGTGGGAGTACAGGAACTGTTAGGAATCTACGAATTCGAGAGGATACTGCAAAATATCTTCTCAATCTCGATGTAAATTCAGCCCATTATGACCCCAAGACCAGGTCCATGCGTGAAGATCCCCTTCCCGAGGCTGATCCAAATGAAAAGTTTTACTTGGGTGATAACCAATATAGAAATAGTGGTCAAGCTTTGGAATTCAAAGACTTGAACATCCATGCTTGGGAAGCATTTGACAAGGGACAAGATGTTCACATGCAAGCAGCTCCTTCCCAAGCCGAATTGCTCTATAAGAATTTCAAGGTCATGAAGGACAAATTGAAATCTCAAACAAAGGAAACTATTATCGAGAAGTATGGCAATGCAGCTGATGATGACAAGCTTCCAAGAGAACTTCTGCTTGGTCAGAGTGAGAGGCAAGTTGAGTATGATCGTGCTGGTAGAATTATTAAGGGACAGGAAGCTGCAATCCCCAGAAGCAAGTATGAAGAAGATGTTTACATTAACAACCACACAACTGTTTGGGGTTCATGGTGGAAGGATCACCAATGGGGATTTAAGTGCTGCAAGCAGACAATACGTAATAGTTATTGTACTGGTACTGCTGGTATTGAGGCTGCTGAAGCTGCAAATGATCTTATGAGAGCTAATATTGCTCGTAAAGAAGCTGCTGCAGAGGATCCTACACCAGTAGAAGAGAAAAGGCCTGCTACATGGGGAAGTGATGTCCCAGAGGACCTAGTTCTGGATGAAAAATTGCTTGCGGATGCACTGAAAAAGGAGGATCAGAGAAAGAGAGAAGAGAAAGATGAGAGGAAGCGTAAATATAATGTAAAATGGAATGATGAGGTTACTCAAGAGGACATGGAGGCATACAGGATGAAGAAAGTACATCATGATGATCCAATGAGTAAATTCATGAACTAAGCCACAAATCTTCCTGTGGTTTTCTCACTGTCAGAAGGATTGTGATGATTAATGAAAAAGAGGAGCAGAATAGAACAGCTTCTTGGATAGTTATTATAGTTTTTGGAACTCAATAGCATCTTATAACTTCCAGCGTGACATGTATATTCACCGATTACTTGTCTGTGTGTGAGAGAGAGAACTTGGGGCAGATGATTTACCAGTTTAAGTACTAGTATGTTTTACTTGTCCTTGAGAGACCTTCAGTTTAAGTATATCCACGTAGTATTGACTGTGTYGTTTACCTGCTACTAAAATTTGTTTTTAATAGTAAAATGTATTTACTTAAGTTTAAAGTTAAAAGTTT
TARGET=1611,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04018 Ps_contig_mira-and-tgicl-ass_franssen_31849
SEQUENCE=CGGGGTTAGTTAATTTGGTAACGNACATTAGTATGGTAGTTTTGTGACCGTGGATCCCTCGATCTGAATAAACCCTCATTGGGATTCTCTTCACTTGCGGCAGCGACTCACACAGAAAGAGAGAAAAATCAAATCAGAGCTTTCTTCAATTCAATTCAATGGAGAAAGGAAAGGGTGCGACGGGATCATCTGGAAGAAGATGGGCCGTTGATTTCTCCGATAATTCCACTTCTCGCGATATTCTCGATCCTCCTGGTTTCTCACGCGCTTCCCTCGATCAAGACGATTCAACTCTTAGTCGCCAGAAGAAGGATGCTGAATCCAGTTGGAAGTCTCAGAAAGCTTGGGAAGTTGCACAAGCCCCTTTTAAGAATTTGTTAATGATGGGGTTCATGATGTGGATGGCTGGTAACACTGTTCACCTGTTTAGTATTGGTATTACCTTCTCAGCTCTTTGGCAACCCATTAGTGCCTTACAAACTGTTGGAAAGATGTTTGAACCTTACAAAGATGCCAAAGTGGAGCTTCTTGGGCCTAAGTTGTTATTCATTGCCCTTAATTTGGGAGGCATGGCACTAGGTGTTTGGAAGCTCAATTCATTGGGACTGCTTCCAACTCACACATCAGATTGGGTGTCGTCCCTAGCACCTGCTCAGGAGGTGGAATACTCAGGTGGTGGTCTAAATTTTGGTTGATGCTCTAGTTCTTTCCTTTTTGGTGGCTTCACTTGAGGAAATTAAGGATTTGACGCAGTTAGAATGTTGTAACAGTCACCAATATTAAACTGGGTTTTTAGTAAGACTAAAAGTGCTCTGAATAACATTTCTATTTGTATTTAAATTATAGTATTTGCACATTATATATCTCTATCTCTTCTTCAAATCTGATGTAAAGGAAAATGGCATACTAGAATCTCCCTTTCAATGAAACATCTAAACTTTTGATTTCTTTTAAATTNGCATTTTTTAGTTAAG
TARGET=145,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1850
PRIMER_LEFT_PENALTY=0.182645
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=AAACCCTCATTGGGATTCTCTT
PRIMER_RIGHT_SEQUENCE=TTCCCAAGCTTTCTGAGACTTC
PRIMER_LEFT=69,22
PRIMER_RIGHT=349,22
PRIMER_LEFT_TM=60.183
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.2100
PRIMER_LEFT_1_PENALTY=0.207669
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=GCGACTCACACAGAAAGAGAGA
PRIMER_RIGHT_1_SEQUENCE=TTCCCAAGCTTTCTGAGACTTC
PRIMER_LEFT_1=102,22
PRIMER_RIGHT_1=349,22
PRIMER_LEFT_1_TM=59.792
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.3342
PRIMER_LEFT_2_PENALTY=0.182645
PRIMER_RIGHT_2_PENALTY=0.151533
PRIMER_LEFT_2_SEQUENCE=AAACCCTCATTGGGATTCTCTT
PRIMER_RIGHT_2_SEQUENCE=CTGGATTCAGCATCCTTCTTCT
PRIMER_LEFT_2=69,22
PRIMER_RIGHT_2=324,22
PRIMER_LEFT_2_TM=60.183
PRIMER_RIGHT_2_TM=59.848
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.3354
PRIMER_LEFT_3_PENALTY=0.182645
PRIMER_RIGHT_3_PENALTY=0.152712
PRIMER_LEFT_3_SEQUENCE=AAACCCTCATTGGGATTCTCTT
PRIMER_RIGHT_3_SEQUENCE=GATCGAGAATATCGCGAGAAGT
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=248,22
PRIMER_LEFT_3_TM=60.183
PRIMER_RIGHT_3_TM=59.847
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.3516
PRIMER_LEFT_4_PENALTY=0.207669
PRIMER_RIGHT_4_PENALTY=0.143968
PRIMER_LEFT_4_SEQUENCE=GCGACTCACACAGAAAGAGAGA
PRIMER_RIGHT_4_SEQUENCE=TCCTTCTTCTGGCGACTAAGAG
PRIMER_LEFT_4=102,22
PRIMER_RIGHT_4=312,22
PRIMER_LEFT_4_TM=59.792
PRIMER_RIGHT_4_TM=60.144
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_04019 Ps_contig_mira-and-tgicl-ass_franssen_31850
SEQUENCE=AAGCAGTGGTATCAACGCTAGTNGCNAGGTCGGGGTTGAGTTCGTTCATTCTATCTGATAAACCATCTTATCCACACTCTTCCGAAACTCTTACAAACATGGCTTCAACCAACGCTCTATCCTCTACCTCAATTCTTCGTTCACCAACCAACCAGGCACAGACCAGTCTCAGCAAGAAGGTTAAACAACATGGAAGGGTCAATTTCAGGCAGAAACCTAACCGTTTTGTGGTTAAAGCCGCCGCTAAAGATATCGCCTTTGACCAACATTCACGCTCCGCTATGCAAGCCGGAATTGATAAACTTGCCGATGCTGTTGGCCTCACTCTTGGACCCAGAGGGAGGAATGTTGTGTTGGATGAGTTTGGAAGTCCTAAGGTAGTTAATGATGGTGTGACAATTGCAAGAGCTATTGAGCTTCCTGATCCCATGGAAAATGCTGGTGCAGCTCTTATAAGGGAGGTTGCAAGTAAAACTAATGACTCTGCTGGCGACGGGACTACAACGGCTTCGATTCTGGCTAGGGAAATTATAAAGCTTGGACTTCTCAATGTAACATCGGGTGCTAATCCTGTGTCGATCAAGAAAGGAATTGATAAAACTGTGGCGGCTTTGGTGGAAGAGCTTGAGAAGTTGGCTAGGCCTGTTAAAGGTGGAGATGATATAAAAGCTGTTGCTACTATTTCTGCTGGAAATGATGAGTTGATTGGAAAAATGATTGCTGAAGCGATTGACAAGGTTGGACCCGATGGTGTTTTATCCATTGAGTCTTCTAACTCGTTTGAGACGACAGTTGAAGTTGAAGAAGGAATGGAGATTGACAGAGGATATATCTCCCCTCAGTTTGTAACAAATCCAGAGAAATCAATTGTTGAATTTGAGAACGCCAGAGTCTTGATTACCGATCAGAAGATTTCAGCAATCAAAGATATCATTCCTCTGCTGGAGAAAACCACTCAATTGAGAGCCCCCTTGCTTATCATTTCTGAAGATATCACTGGTGAGGCTTTGGCGACCCTTGTTGTCAATAAGCTGCGTGGTATCCTTAATGTCGCTGCCATCAAAGCTCCAGGATTTGGTGAAAGAAGAAAGGCCCTTCTTCAAGACATTGCTATATTGACAGGTGCTGAGTTCCAAGCCAGTGATCTGGGCCTTCTCGTTGAAAACACCACAATTGAACAACTTGGTTTGGCCCGGAAGGTGACCATCTCCAAGGATTCTACCACTATTATTGCCGATGCTGCATCAAAGGATGAGTTGCAATCCAGGGTTGCACAACTAAAGAAAGAGTTGTCTGAGACAGACTCAATTTATGATTCTGAGAAACTCGCCGAGAGAATTGCTAAGTTGTCTGGTGGAGTTGCTGTCATCAAGGTCGGTGCTGCCACAGAGACCGAACTGGAGGATCGTAAGCTCCGTATTGAGGATGCCAAGAATGCAACTTTTGCCGCCATTGAGGAAGGTATTGTTCCTGGTGGAGGCACTGCATTGGTTCATCTATCTGGTTATGTCCCAGCAATTAAGGAGAAGCTTGAAGATGCAGATGAGAGGCTAGGAGCTGACATTGTTCAAAAGGCATTGGTAGCACCTGCTGCATTGATTGCTCAAAATGCTGGAATAGAGGGTGAAGTAGTTGTGGAGAAAATTAAGAATGGTGAATGGGAGGTTGGTTACAATGCCATGACAGACACTTACGAGAATTTAGTGGAGTCTGGAGTCATTGACCCTGCCAAGGTAACAAGGTGTGCTTTGCAGAATGCTGCTTCAGTAGCCGGGATGGTCTTAACCACACAGGCCATCGTCGTCGAAAAGCCCAAGCCCAAAGCAGCCGTGGCTGCTGCCCCACAAGGCCTTACTATTTAAGTCTTAGGTTAACTATTGTTGTAGGTTGAGTACCGCAAAAACAGTTTTGAGCAGAAAAGTGAAAGAGCTTTTGTTTCGGATGCCTGTTCTATTAAAAGGAAAAACAAGCTGTATTTGTAAACATAGATTAAGATTTGCTTTGGTTGACGAGGGATAAGGGAAGTGATAAATTTGGATTCAAGTCAAAANGNAAATAAAAAATTATAAGTAACTTTAAAAAGG
TARGET=143,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1038
PRIMER_LEFT_PENALTY=0.095538
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=TACAAACATGGCTTCAACCAAC
PRIMER_RIGHT_SEQUENCE=CTTCCAAACTCATCCAACACAA
PRIMER_LEFT=91,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.1911
PRIMER_LEFT_1_PENALTY=0.095538
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=TACAAACATGGCTTCAACCAAC
PRIMER_RIGHT_1_SEQUENCE=GAAATTGACCCTTCCATGTTGT
PRIMER_LEFT_1=91,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=0.2052
PRIMER_LEFT_2_PENALTY=0.109631
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=GCAGTGGTATCAACGCTAGTNG
PRIMER_RIGHT_2_SEQUENCE=GAAATTGACCCTTCCATGTTGT
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=205,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.50
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0250
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=204
PRIMER_PAIR_PENALTY_3=0.2202
PRIMER_LEFT_3_PENALTY=0.095538
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=TACAAACATGGCTTCAACCAAC
PRIMER_RIGHT_3_SEQUENCE=TTCCAAACTCATCCAACACAAC
PRIMER_LEFT_3=91,22
PRIMER_RIGHT_3=368,22
PRIMER_LEFT_3_TM=59.904
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=0.2703
PRIMER_LEFT_4_PENALTY=0.261998
PRIMER_RIGHT_4_PENALTY=0.008257
PRIMER_LEFT_4_SEQUENCE=CTTCAACCAACGCTCTATCCTC
PRIMER_RIGHT_4_SEQUENCE=CTTCCAAACTCATCCAACACAA
PRIMER_LEFT_4=102,22
PRIMER_RIGHT_4=369,22
PRIMER_LEFT_4_TM=60.262
PRIMER_RIGHT_4_TM=60.008
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_04020 Ps_contig_mira-and-tgicl-ass_franssen_31855
SEQUENCE=TGGAAGTAATAAAGTCAACCTTAATGAATCTAAGTTTTTAGTTTGTTTTTTGTTTTTCCTTCTTAATACTTGGGTGCTTATCTCCAATTTCAAGCTAGCTTACAATCTTCTAAGACGGGCGGATGGAACTTTCAATAGAGAGTTGGCTGAGTTTCTCGACCGCAAGGTTCCGGCCAATACAATTCCGGTTGACGGAGTATTTTCTTTCGATCATGTCGATCGGAATACAGGACTTTTTAGTAGGGTTTATCAACCTGCTCCAGAGAATGTTACTACTTGGGGCATTATAGAGCTAGAAAAGCCCTTGAGCACAACTGAGATTGTCCCTGTCATAATTTTCTTCCATGGTGGAAGCTTTTCTCATTCCTCAGCTAATAGTGCTATCTACGACACTTTCTGTCGTCGCCTTGTGAGCATGTGTAAGGCTGTTGTTGTTTCTGTAAACTACCGAAGATCGCCCGAGCATCGGTTTCCATGTGCTTATGAAGACGGTTGGAATGCTCTTAAATGGGTTAAATCAAGGACATGGCTTCAAAGTGGAAAAGATTCTAAGGTTTATGTCTACATGGCAGGGGATAGTTCCGGTGGTAACATTGCTCATCATGTCGCGGTGAGAGCTGCCGAGGAAGATGTCGAGGTACTAGGTAACATTCTTCTCCATCCGCTCTTTGGCGGGGAGAAGCGTACGGAATCAGAGAAGAAACTGGACGGAAAGTATTTTGTGAGGTTGCAAGATCGCGATTGGTATTGGAGAGCTTTTCTACCCGAAGGAGAAGATAGAGACCATCCTGCTTGCAATCCATTCGGACCTAATGGTAAACGCCTTGAAGGACTAAAGTTCGCTAAAAGCCTTGTTTGCGTGGCCGGTTTGGATCTTCTTCAAGATTGGCAATTGGAATATGTGGAAGGTCTCAAGAATTATGACCAAGATGTTAAACTTCTTTACCTAAAGGAAGCAACTATTGGTTTCTACTTCTTGCCTAATAATGATCATTTCTATTGCCTCATGAACGAGATTAACACCTTCGTGCATCCTAACTGTTGATAAATCGCCACTTCTCTACTACTACTACTACTTAACCATACATGACTAAAAGCCTGACTTGTATTCTTTATGTCCCTACTTTACAGTGGTGGTGTGTAGTTATTATAATATTATGTAGTGTTGTTACTCACTGTTTTAACATCTATGGTGGTAAAAATCTGAGCTTTCTGTTATCCGCGGACTACTTGTTGGTGCACTCTGCCTATATCTTTTGAAGGGTAGCACCGGTTTCTGCGTCTGAGGTACCGGAGTTTGGTTGTGCCACTGTACTCAAAGCTTGCCCCAATTTACTTGTTCGACCTCGAAAGGAGGAGGGAAGATTCAAGATAAGGCATGGTTGATGAATTTGATTAAGTGAGTGTTTTTATGTTGTATAACTATTATATAGAGTGGGAACTTTTGAGTATAGCCCATCCTGTTATGAACTTGTATGTTTCTTATATAGTAATATAAGTGTCTTGTTTGTTTGATTTTATCTTTGTACCAAAATGGTGAAGTGTTGTAGTGTTCTTTGTTGTTATTTTCAATTTCTTGACACAGAAGTTACATATGTATTTGGTTTTCTTGGNGNGTNTANATNTANAGAATATTGGTACGAACAGTGGTAGTA
TARGET=1061,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1773
PRIMER_LEFT_PENALTY=0.060824
PRIMER_RIGHT_PENALTY=0.116503
PRIMER_LEFT_SEQUENCE=AACACCTTCGTGCATCCTAACT
PRIMER_RIGHT_SEQUENCE=GCGGATAACAGAAAGCTCAGAT
PRIMER_LEFT=1018,22
PRIMER_RIGHT=1222,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=59.883
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.2100
PRIMER_LEFT_1_PENALTY=0.060824
PRIMER_RIGHT_1_PENALTY=0.149184
PRIMER_LEFT_1_SEQUENCE=AACACCTTCGTGCATCCTAACT
PRIMER_RIGHT_1_SEQUENCE=AGTAGTCCGCGGATAACAGAAA
PRIMER_LEFT_1=1018,22
PRIMER_RIGHT_1=1230,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.2100
PRIMER_LEFT_2_PENALTY=0.060824
PRIMER_RIGHT_2_PENALTY=0.149184
PRIMER_LEFT_2_SEQUENCE=AACACCTTCGTGCATCCTAACT
PRIMER_RIGHT_2_SEQUENCE=AAGTAGTCCGCGGATAACAGAA
PRIMER_LEFT_2=1018,22
PRIMER_RIGHT_2=1231,22
PRIMER_LEFT_2_TM=60.061
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.2100
PRIMER_LEFT_3_PENALTY=0.060824
PRIMER_RIGHT_3_PENALTY=0.149184
PRIMER_LEFT_3_SEQUENCE=AACACCTTCGTGCATCCTAACT
PRIMER_RIGHT_3_SEQUENCE=GTAGTCCGCGGATAACAGAAAG
PRIMER_LEFT_3=1018,22
PRIMER_RIGHT_3=1229,22
PRIMER_LEFT_3_TM=60.061
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.2714
PRIMER_LEFT_4_PENALTY=0.154878
PRIMER_RIGHT_4_PENALTY=0.116503
PRIMER_LEFT_4_SEQUENCE=TTCTATTGCCTCATGAACGAGA
PRIMER_RIGHT_4_SEQUENCE=GCGGATAACAGAAAGCTCAGAT
PRIMER_LEFT_4=994,22
PRIMER_RIGHT_4=1222,22
PRIMER_LEFT_4_TM=59.845
PRIMER_RIGHT_4_TM=59.883
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_04021 Ps_contig_mira-and-tgicl-ass_franssen_31859
SEQUENCE=TGAAGCCGTTGAGGCAAATCTTCCTTCAAAAACACCTTCAACTTGTTCCCATTTTCAACTTTCCCTGAGCTCAATCCTTCATTCATCTTAACCACAACATCTTCCGACGAATAATTACCCGTCGGCCAAATCGCAAGCAAGGGCGAGTACGAATGAACCCAACTCCGTTGAATCTTAACCCAAGGAGATAAATCATCTAAAAAAACAAGCTTCTGATCACAAGTACCAACCATACCATGATCACCAACCATAATAATTGTAACATCTTCAAAAACCCTTTTTGTTCCAACCCATTAATAAGCCTCCCAATAATAGAATCAATCCTAGAAACAGCATCAGTAATCTCATCATCATCATCAGGTCCAACTTGATGACCTTGATGATCAGGGTCCTCAAAATAAAGAGTCATAAAAACAGGAATTTCATCAAGTGGCAAATCAAAATACTCCAAAACAGAATCAACTCTATCTTCAAAACCAACAGAACCATTATAATGCTTACAAAATTGATCAGGACAAGTCCATGAGCCTTTGATTACCTCAGATCCAGGCCAAAA
TARGET=345,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3660
PRIMER_LEFT_PENALTY=0.005674
PRIMER_RIGHT_PENALTY=0.360320
PRIMER_LEFT_SEQUENCE=CGTTGAATCTTAACCCAAGGAG
PRIMER_RIGHT_SEQUENCE=GCCACTTGATGAAATTCCTGTT
PRIMER_LEFT=165,22
PRIMER_RIGHT=433,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.360
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=269
PRIMER_PAIR_PENALTY_1=0.3660
PRIMER_LEFT_1_PENALTY=0.005674
PRIMER_RIGHT_1_PENALTY=0.360320
PRIMER_LEFT_1_SEQUENCE=CTCCGTTGAATCTTAACCCAAG
PRIMER_RIGHT_1_SEQUENCE=GCCACTTGATGAAATTCCTGTT
PRIMER_LEFT_1=162,22
PRIMER_RIGHT_1=433,22
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=60.360
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.3827
PRIMER_LEFT_2_PENALTY=0.022341
PRIMER_RIGHT_2_PENALTY=0.360320
PRIMER_LEFT_2_SEQUENCE=CAACCATACCATGATCACCAAC
PRIMER_RIGHT_2_SEQUENCE=GCCACTTGATGAAATTCCTGTT
PRIMER_LEFT_2=226,22
PRIMER_RIGHT_2=433,22
PRIMER_LEFT_2_TM=59.978
PRIMER_RIGHT_2_TM=60.360
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.4180
PRIMER_LEFT_3_PENALTY=0.057688
PRIMER_RIGHT_3_PENALTY=0.360320
PRIMER_LEFT_3_SEQUENCE=ACCCATTAATAAGCCTCCCAAT
PRIMER_RIGHT_3_SEQUENCE=GCCACTTGATGAAATTCCTGTT
PRIMER_LEFT_3=288,22
PRIMER_RIGHT_3=433,22
PRIMER_LEFT_3_TM=59.942
PRIMER_RIGHT_3_TM=60.360
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.4359
PRIMER_LEFT_4_PENALTY=0.075553
PRIMER_RIGHT_4_PENALTY=0.360320
PRIMER_LEFT_4_SEQUENCE=TGTTCCAACCCATTAATAAGCC
PRIMER_RIGHT_4_SEQUENCE=GCCACTTGATGAAATTCCTGTT
PRIMER_LEFT_4=281,22
PRIMER_RIGHT_4=433,22
PRIMER_LEFT_4_TM=60.076
PRIMER_RIGHT_4_TM=60.360
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_04022 Ps_contig_mira-and-tgicl-ass_franssen_31864
SEQUENCE=CCTACTCACTCTCTCTCTCTCTCTCTCTCTCTTCCTCTCTTCTTCTTCTTCTTCTGAGAGAGAACTAGAAGAGCTCAAAAACCCTAAACCCTTCAACTTCATCATTCCAAAGAATGCAGAAAGCCACTCTATTCCTCTCACTACTCTTCCACTTCTTCTCAACCATAACCTCAAAATCAACAATCGAACCATGCACAAACTCCGACACATGCAACGCTTTACTCGGTTACACACTCTACACAGACCTCAAAGTCTCCGAAGTTTCATCTCTCTTCCAAATCGACCCTATCTCTCTCCTAACCGCCAATGCCATTGACATCTCTTACCCCGACGTAGAACACCACATCCTCCCTTCCAAACTCTACCTCAAAATCCCAATCTCATGTTCCTGTGTCGACGGGATTCGCAAATCCGTTTCCACCAACTACAAAATTCGTCCCTCTGACACTCTTTCCACCATTGCTGACTCTGTTTACGGTGGTTTGGTTTCTTCTGACCAGCTTAAAGAGGCTAATTCTATTTCCGATCCTTCTGTTCTTGATGTTGGACAGAACCTTGTTGTTCCTCTTCCTTGTACTTGTTTCAATGGTTCTGATAATGCTTTGCCTGCTATTTATCTGTCTTATGTTGTTCAGCCACTTGATAGTTTGAGTTATATTGCTGCTAGATATTTTACTACTCTTACCGATTTGATGAATGTTAATGCTATGGGAAGTACTTCTATCTCTGATGGTGATATTCTTGCTATTCCTATTCCGGCATGTGCTTCAAATTTTCCAAAATATGCTTCTGATTTCGGCTTGCTTGTCCCTAATGGAAGTTATGCCATTACAGCAGGCCACTGTGTGCAATGTAGCTGTGGACCCCGAAACTTAAATTTGTACTGTATGCCGGCTTCTCTAGCTGTTTCTTGCTCCAGCATGCAATGTAAAAGCAGCAATCTTATGCTTGGCAATGTTACCGTGCAACAGACTAGTGGTGGTTGTAATGTGACTTCTTGCAGTTATGATGGTCTTGTTAATGGCACCATAGCGACAATGTTGACACCGTCTCTTCAGCCTCGATGCCCAGGGACACAGGAATTTCCTCCTCTTATCGGCCCCACCTACAACTGTTGCGAGAGAATCAGTATTTGCACCGGCACCGTCTCCATCATCGCTGTTAGATGGAACTGATGGGAGATCACCCAAATCCTCTGTGGTGCCTTCAACAGGATTTACTCCGGCTAATGGCCCTGTTAGTGGAATTTCTTCTGGTGCTTCTGCTGCATGCTCTTTGCTGAAACCATTACCCATTTTGACAGTTCCACTTGTGTTATTACTTGTCAAGTTGATGATCCCCTTAGCATTGTAATCTTGTCAGTTTTGCGGTGGGTTTAATTATGTCTTTGCCACAGGTGCTCCTATCTATTGTAGTTGCTAGTATTCATTTGTACTCTTTAACTGATTGTGAATCTAGGAAAACTATGGTAAATACGGGTTTAAAATACTNG
TARGET=9,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04023 Ps_contig_mira-and-tgicl-ass_franssen_31864
SEQUENCE=CCTACTCACTCTCTCTCTCTCTCTCTCTCTCTTCCTCTCTTCTTCTTCTTCTTCTGAGAGAGAACTAGAAGAGCTCAAAAACCCTAAACCCTTCAACTTCATCATTCCAAAGAATGCAGAAAGCCACTCTATTCCTCTCACTACTCTTCCACTTCTTCTCAACCATAACCTCAAAATCAACAATCGAACCATGCACAAACTCCGACACATGCAACGCTTTACTCGGTTACACACTCTACACAGACCTCAAAGTCTCCGAAGTTTCATCTCTCTTCCAAATCGACCCTATCTCTCTCCTAACCGCCAATGCCATTGACATCTCTTACCCCGACGTAGAACACCACATCCTCCCTTCCAAACTCTACCTCAAAATCCCAATCTCATGTTCCTGTGTCGACGGGATTCGCAAATCCGTTTCCACCAACTACAAAATTCGTCCCTCTGACACTCTTTCCACCATTGCTGACTCTGTTTACGGTGGTTTGGTTTCTTCTGACCAGCTTAAAGAGGCTAATTCTATTTCCGATCCTTCTGTTCTTGATGTTGGACAGAACCTTGTTGTTCCTCTTCCTTGTACTTGTTTCAATGGTTCTGATAATGCTTTGCCTGCTATTTATCTGTCTTATGTTGTTCAGCCACTTGATAGTTTGAGTTATATTGCTGCTAGATATTTTACTACTCTTACCGATTTGATGAATGTTAATGCTATGGGAAGTACTTCTATCTCTGATGGTGATATTCTTGCTATTCCTATTCCGGCATGTGCTTCAAATTTTCCAAAATATGCTTCTGATTTCGGCTTGCTTGTCCCTAATGGAAGTTATGCCATTACAGCAGGCCACTGTGTGCAATGTAGCTGTGGACCCCGAAACTTAAATTTGTACTGTATGCCGGCTTCTCTAGCTGTTTCTTGCTCCAGCATGCAATGTAAAAGCAGCAATCTTATGCTTGGCAATGTTACCGTGCAACAGACTAGTGGTGGTTGTAATGTGACTTCTTGCAGTTATGATGGTCTTGTTAATGGCACCATAGCGACAATGTTGACACCGTCTCTTCAGCCTCGATGCCCAGGGACACAGGAATTTCCTCCTCTTATCGGCCCCACCTACAACTGTTGCGAGAGAATCAGTATTTGCACCGGCACCGTCTCCATCATCGCTGTTAGATGGAACTGATGGGAGATCACCCAAATCCTCTGTGGTGCCTTCAACAGGATTTACTCCGGCTAATGGCCCTGTTAGTGGAATTTCTTCTGGTGCTTCTGCTGCATGCTCTTTGCTGAAACCATTACCCATTTTGACAGTTCCACTTGTGTTATTACTTGTCAAGTTGATGATCCCCTTAGCATTGTAATCTTGTCAGTTTTGCGGTGGGTTTAATTATGTCTTTGCCACAGGTGCTCCTATCTATTGTAGTTGCTAGTATTCATTTGTACTCTTTAACTGATTGTGAATCTAGGAAAACTATGGTAAATACGGGTTTAAAATACTNG
TARGET=38,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=5.0943
PRIMER_LEFT_PENALTY=5.010445
PRIMER_RIGHT_PENALTY=0.083883
PRIMER_LEFT_SEQUENCE=TCTCTCTCTCTCTCTCTCTTCCTCT
PRIMER_RIGHT_SEQUENCE=CTTCGGAGACTTTGAGGTCTGT
PRIMER_LEFT=13,25
PRIMER_RIGHT=260,22
PRIMER_LEFT_TM=57.990
PRIMER_RIGHT_TM=59.916
PRIMER_LEFT_GC_PERCENT=48.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=0.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=5.0943
PRIMER_LEFT_1_PENALTY=5.010445
PRIMER_RIGHT_1_PENALTY=0.083883
PRIMER_LEFT_1_SEQUENCE=CTCTCTCTCTCTCTCTCTCTTCCTC
PRIMER_RIGHT_1_SEQUENCE=CTTCGGAGACTTTGAGGTCTGT
PRIMER_LEFT_1=12,25
PRIMER_RIGHT_1=260,22
PRIMER_LEFT_1_TM=57.990
PRIMER_RIGHT_1_TM=59.916
PRIMER_LEFT_1_GC_PERCENT=52.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=0.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=5.0943
PRIMER_LEFT_2_PENALTY=5.010445
PRIMER_RIGHT_2_PENALTY=0.083883
PRIMER_LEFT_2_SEQUENCE=TCTCTCTCTCTCTCTCTCTCTTCCT
PRIMER_RIGHT_2_SEQUENCE=CTTCGGAGACTTTGAGGTCTGT
PRIMER_LEFT_2=11,25
PRIMER_RIGHT_2=260,22
PRIMER_LEFT_2_TM=57.990
PRIMER_RIGHT_2_TM=59.916
PRIMER_LEFT_2_GC_PERCENT=48.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=0.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=5.0943
PRIMER_LEFT_3_PENALTY=5.010445
PRIMER_RIGHT_3_PENALTY=0.083883
PRIMER_LEFT_3_SEQUENCE=CTCTCTCTCTCTCTCTCTCTCTTCC
PRIMER_RIGHT_3_SEQUENCE=CTTCGGAGACTTTGAGGTCTGT
PRIMER_LEFT_3=10,25
PRIMER_RIGHT_3=260,22
PRIMER_LEFT_3_TM=57.990
PRIMER_RIGHT_3_TM=59.916
PRIMER_LEFT_3_GC_PERCENT=52.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=0.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=5.2306
PRIMER_LEFT_4_PENALTY=5.010445
PRIMER_RIGHT_4_PENALTY=0.220131
PRIMER_LEFT_4_SEQUENCE=TCTCTCTCTCTCTCTCTCTCTTCCT
PRIMER_RIGHT_4_SEQUENCE=TGTGTAACCGAGTAAAGCGTTG
PRIMER_LEFT_4=11,25
PRIMER_RIGHT_4=232,22
PRIMER_LEFT_4_TM=57.990
PRIMER_RIGHT_4_TM=60.220
PRIMER_LEFT_4_GC_PERCENT=48.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=0.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_04024 Ps_contig_mira-and-tgicl-ass_franssen_31865
SEQUENCE=AGTTAAATAATACCTATAAAATTAGTNTAAATAAAATTGTGAGTTCGTAATGAATTATAAATTATAAAACTAGGACGTTTACAGCCTTGAATTTTGGCTTTAAATAAAATGCCATGCACTAGCAGCCCTACCCTCTGTTTCTAGAAAGTTGCGAGGGTTTCATCTAAAAACGGGAAAGGATATGGCAAGCAAACTAACAAGCCTAAGAGCAAAACTGTTACTCCAAGAGCAACATACTTGTCATCAGGTTCGCTTATTTCATCAGATAGAGGAGCAATTGGACCTCTTTGCAAGAAGAATATAACAACTACCCAGTAGAATGCTACATCATTAAGTAGTGATGACAGTCCAAGCAACACAATTGAAAATCCTGTCAAGCGAATTGAAGCCTTTCTTCCCCATAAGGCAAAAGATATTCTTCCTCCATCAAGCTCTCCAGCAGGAATACTGTTGATGGCATTGATAAGGAGTCCTGCCCAAGCCCAAATCACAAGAGGGTTCACAGAAATTGGAGTTCCTTCCTTGAGAAGGTTGCCAAGAAGCAGTTTTGCAATGCCACCAGCAAGAAATGACTCGTGAAACACAGAAGCATCAACAACAACACCAATACCGTCGCTTGGGGGTATAACAAAACCTACAAGCAGAAGCACAAGACCAACTGTAAATCCAGCTATTGGTCCAGCAGCTGCAATCTTGAGTAAATCTTGGCGATTAGATACTATGCTTCTTATCCTTGTAATTGAACCAAAAGAGCCTATCTGCCAGCTAGGAATAAAGTACGGGACCCCGAGCTTAACTCCAGTGTTTTGGGCAACTAGAAAGTGAGCAAGTTCATGCACCCCCAGAATAAGTGCAGTAACAAGGGCTCCAGGTAGACCATCCTTTAACAAGTTCAGATTGTCATAAGTTGATAGTAGATTGGATTGCAAATCTGGAACATTGCGAAGCAGCAAGGTAAACAATGTGACCAGTCCAAAAGATCCAGCAGCAAACCACTCAGGTACAGCAGTTGTTTCTGGTTGTAAGGTAGTTCTTGGCACAACAACGGCCACCGGCTTATCATCCTCGGGATTGACTAGAAGGAAAAGTCTATATTCATCTCCAAATTTATCTTGCAATCTCTTTGATATTTTATCATAACTTTTAGAAGCTTGCCCTCGCAGGTTTCCCTTGAACAACACTCCACCCTCGTAAGGATCTTGGCTTGTCACAAAAAAAGTATCAAAGCCAAACACTTGATTTTTAAAGATTTCAATTGTTTCCTTGGGAATTTTTATTACATCACCCAGTTGTTGAGGCTTCACACCTGGCAGAGGTGATCCACTGGCAACTTCTATGTTATCCATATTCTGTGTATCATTTTGTTCTGTATTTCCATCACTAAACTTGTTTAGCTGTTCTCCATCTATAGGCGGGGGCTTAATTTGCTCAACAGAATCCTCCAAAGATTGTGTTTCTTCATTTTTATGTTCTTCTTTATCCTCCTGGTTGTCACCGTCTGACTCAGTAACACTACAAGCAATCCTTCTTCTTCTTCCGGTAAGACTCAATTTTCCGGAGCTCCAACGTGCATGGCGAAAACCGTTGGAACGATGAAATCGAAGATTGAACTGAAAGCAGGAAGTGCAGTGCGAGAGAGGAACGACGAGGCTCCCGCGAAAGGTAGAAGGTGATGGTAGTGAGAGAATCATGGTTTCGTTGGGAGAAAAAATGAGTCTAGGTTGAATGAAACCCTAATGCCATTAAAATGAGTGAGGATGGAGAAAATGGCAGAGAACTGAAGAAGTAGCGATAGGAAGGGAGCGACGCGAGGCAAGGAGAACCAATTATCCGCTTTTTTTTTGGTTCCCCCGTNCTCTGCGTTGATATTACCTACTGCTT
TARGET=1525,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2464
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.110392
PRIMER_LEFT_SEQUENCE=CTTTATCCTCCTGGTTGTCACC
PRIMER_RIGHT_SEQUENCE=CAACGAAACCATGATTCTCTCA
PRIMER_LEFT=1474,22
PRIMER_RIGHT=1700,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.5122
PRIMER_LEFT_1_PENALTY=0.401769
PRIMER_RIGHT_1_PENALTY=0.110392
PRIMER_LEFT_1_SEQUENCE=AATTTGCTCAACAGAATCCTCC
PRIMER_RIGHT_1_SEQUENCE=CAACGAAACCATGATTCTCTCA
PRIMER_LEFT_1=1421,22
PRIMER_RIGHT_1=1700,22
PRIMER_LEFT_1_TM=59.598
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.6124
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.476376
PRIMER_LEFT_2_SEQUENCE=CTTTATCCTCCTGGTTGTCACC
PRIMER_RIGHT_2_SEQUENCE=ACTGCACTTCCTGCTTTCAGTT
PRIMER_LEFT_2=1474,22
PRIMER_RIGHT_2=1629,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.476
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.7059
PRIMER_LEFT_3_PENALTY=0.595491
PRIMER_RIGHT_3_PENALTY=0.110392
PRIMER_LEFT_3_SEQUENCE=GGGCTTAATTTGCTCAACAGAA
PRIMER_RIGHT_3_SEQUENCE=CAACGAAACCATGATTCTCTCA
PRIMER_LEFT_3=1415,22
PRIMER_RIGHT_3=1700,22
PRIMER_LEFT_3_TM=60.595
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=0.7383
PRIMER_LEFT_4_PENALTY=0.132262
PRIMER_RIGHT_4_PENALTY=0.606086
PRIMER_LEFT_4_SEQUENCE=TGATCCACTGGCAACTTCTATG
PRIMER_RIGHT_4_SEQUENCE=AGCTCCGGAAAATTGAGTCTTA
PRIMER_LEFT_4=1316,22
PRIMER_RIGHT_4=1561,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=59.394
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_04025 Ps_contig_mira-and-tgicl-ass_franssen_31866
SEQUENCE=ACTTGGATTNTAAACCAAAAAAACATAACTCAGACTAGCACGAAGTTACAAGTTACAGTCTTTGGCAGAACTGCTTACATCAAATTTTCGACACCTTACTGAAATTTTAATAATTCTAAGAGAAAAAAAAACAACAAAAAGGTTACTGAAAAACTAAAAGTTTTAATGTATCAAGTCAGGAGACTATAACACCAGATTTTTTCGAGCATATCTACAGACACATGACCTTCATATACCACCTAGGGAGGGGAGTATTATTTGAACATTTTTTTACAAACTGAGGCTACAAAATGAAACAACCTTGATAATTGTCGCATCTTTATCTCTTGAGAGGTTTGTAAAGGACAACTGTCACATATTTAGACTGGAACCTGTGAGTCAATCCCANGGGCAACAACGGACTTTGAAGCCATGTTTTTCTCTATGAAGACATGATTTAGCACAACATGTTGAGGCTTTGAAGAGGGCCCAGAATCAGCATTCTCCATACCTAGGACTGTAAGATGTAGCTGCGAGGGAACAGCCAATGGCTCTTTTGCAAAATCTTCCTCAGCTGGATATGCTTGACCATAACTAGATTCTGGGGAGAGTGGTGCTTCAAACTCAGAGACGCTTTCAGGATTTTCTGGCACATAATCGTTAGCATCAAGAAGATTGCAGACATTCCCCATCTCATCAGCTACATAAGGAAGATCTGGAATATATCTCTGTTCACCATCGACAATGAACCTGTAATGATATATACCTGATGGAAGGACTATAAGAATTGAGTGATCCTTGCCACCTCGCTGCATTGCCTTCCTGGATGTCCAGTTATCCCAAGATCCCTCCACAGCCACACTGTTACCTCCATAATTCCAAGTGATCATAACAGGGATTCCTTGCTCAGGAGGTTGATGAACAATACCATGAGGCTCATTCTGCCACATTTGATTGAGAAAAGGAGGGCCGTTACCTCTTTGTAATGGAGCTAGGGGAACCTGAGGACCGAAGAGGATCGGCGATCTGGAACGGCGAGGACTCTGAGGCGGACTATTAGCCATTGAATCGGAAGAAAAAGCACGAACGGGAGGACGAGAATCAGGCGCGTGAGGTTCACGGCCACCAAGATCAACACCGTCGGAAATGGCTCCGTCTTCTCTGCCATTAGCGTTTCCCATGGAGAGATTGAGAAACGTGAAAATACAAAACGCAGGAAAAATATTATATATGTGTACGGTTATTGTCGTTTACTGAAAAGGAGAGAAGAATCGCAATGCAGGCGAAGGAGAAGTAAACGACAAATTGTGAGAGTATGAAGAAGTGAAAGTGCCGCGAAGAAGAAGAAGAAAACGGTTCCGTTATTCGTCGTTTTATTCTTATCAAGTTGTGTCTGACTCTGATCTGACGCTGTGATTGTTTTGGACTTTGGAGCGAAGTAAATTTAGATTAAGAAATTAAA
TARGET=1316,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1632
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=GAAAAGGAGAGAAGAATCGCAA
PRIMER_RIGHT_SEQUENCE=ATTTACTTCGCTCCAAAGTCCA
PRIMER_LEFT=1234,22
PRIMER_RIGHT=1422,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=10.5000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.2098
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.175676
PRIMER_LEFT_1_SEQUENCE=GAAAAGGAGAGAAGAATCGCAA
PRIMER_RIGHT_1_SEQUENCE=CAAAACAATCACAGCGTCAGAT
PRIMER_LEFT_1=1234,22
PRIMER_RIGHT_1=1402,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.176
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.5000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.2100
PRIMER_LEFT_2_PENALTY=0.080887
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=GTCTTCTCTGCCATTAGCGTTT
PRIMER_RIGHT_2_SEQUENCE=ATTTACTTCGCTCCAAAGTCCA
PRIMER_LEFT_2=1133,22
PRIMER_RIGHT_2=1422,22
PRIMER_LEFT_2_TM=59.919
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.2566
PRIMER_LEFT_3_PENALTY=0.080887
PRIMER_RIGHT_3_PENALTY=0.175676
PRIMER_LEFT_3_SEQUENCE=GTCTTCTCTGCCATTAGCGTTT
PRIMER_RIGHT_3_SEQUENCE=CAAAACAATCACAGCGTCAGAT
PRIMER_LEFT_3=1133,22
PRIMER_RIGHT_3=1402,22
PRIMER_LEFT_3_TM=59.919
PRIMER_RIGHT_3_TM=60.176
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.2663
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.232153
PRIMER_LEFT_4_SEQUENCE=GAAAAGGAGAGAAGAATCGCAA
PRIMER_RIGHT_4_SEQUENCE=TAAAACGACGAATAACGGAACC
PRIMER_LEFT_4=1234,22
PRIMER_RIGHT_4=1354,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=60.232
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_04026 Ps_contig_mira-and-tgicl-ass_franssen_31868
SEQUENCE=AAAATAAAAACGGATAAACTACAAATTAGTCGGGRTAGAAACGGAACAAAATCAAGAGAGAGAGTGAAAAAAGAGAGTGAGAAAACAGAGATGCGTTGTTATGGCTTATCAATCCCCGGAAGCGACCATCGCTTCTTCCTTCCAACACTTACTCCGTCAGTATCCCTCCGGCCGACGACAACTGTGCCGGTAAACCGTTTAACATTCGGATCAAGTTTTCCGAAACTGAAAGCCACCTTCAACGACGGCGTTGTGACGGAGAAATTGAGTTTCTACGATCTTCTAGGGATTCCCGTATCTGGTTCGTTGAATGATATCAAATCTGCTTATAAACAACTTGCGAGAAAGTATCATCCTGACGTTTCGCCACCGGACCGGGTTGAAGAGTATACGAAAAAGTTTATTCAGGTTCATGAGGCTTATGAGACGTTATCGGATCCTTCAAGGAGAATCATGTATGATCAGGATATGGCTAGAGGCGTGAATCTCGCTTTCAACGCTCGGAAACGCTATAATCACTCCGATCAGGGGAATGAACAAAAAGACGAATGGAAAATCCGTTGGCAATCTCAACTCTCTGGTCTGAAGAGAAGAAGTGACAGCAAGGTTGCTGCTGAGAACATGTCATGGGCTGCTCGAATGCGCCAGCAGAAGGAGGAAATGTGACCAGTAATTTTCCTTTGTTTTCTATGTGTAATAAAAGTTGTATATATCAATTATTTATTCTTTTCGTACTCTACATCAAATCATATATAACATAGTAAACGTTTAAGGAAAAAATTACTATGCACTTTGCAGTATGCTAACCATATAGTTTAACCCAAAAATTGTTGTTTAATTTTGTTACAAATGACTTATAAAGTACTTA
TARGET=55,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04027 Ps_contig_mira-and-tgicl-ass_franssen_31874
SEQUENCE=GGTTCCAATTCAAATTGTTAAACACCAAGAATATCACATCTTCGCTTAAATGACCTTTTGTACATTGGCAGGAAGACGAGTGTGCGCATCGGTACTGCTTCGTGAGAATCATTTCCTTCACCTCTGAGTAGAGGAATTTGGTCCAAAGAAAAGCACAGAAACCAACNTCACACTTGAAAGCTGAGAGAGAGAGAGGGAGGGGGTAGGGAAAGGAAGAAAGAAAAATTAAAAATAAAAAGTAAAAAGGAAAAGAAAATTAAGTTACCTCGTGTCGGTTAGACTACCACTGTCTTC
TARGET=183,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2128
PRIMER_LEFT_PENALTY=0.158297
PRIMER_RIGHT_PENALTY=0.054491
PRIMER_LEFT_SEQUENCE=ATCGGTACTGCTTCGTGAGAAT
PRIMER_RIGHT_SEQUENCE=TTCTTTCTTCCTTTCCCTACCC
PRIMER_LEFT=87,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=60.158
PRIMER_RIGHT_TM=59.946
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=0.3236
PRIMER_LEFT_1_PENALTY=0.269153
PRIMER_RIGHT_1_PENALTY=0.054491
PRIMER_LEFT_1_SEQUENCE=GCTTCGTGAGAATCATTTCCTT
PRIMER_RIGHT_1_SEQUENCE=TTCTTTCTTCCTTTCCCTACCC
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=221,22
PRIMER_LEFT_1_TM=59.731
PRIMER_RIGHT_1_TM=59.946
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.4059
PRIMER_LEFT_2_PENALTY=0.125045
PRIMER_RIGHT_2_PENALTY=0.280862
PRIMER_LEFT_2_SEQUENCE=AAAGCACAGAAACCAACNTCAC
PRIMER_RIGHT_2_SEQUENCE=CAGTGGTAGTCTAACCGACACG
PRIMER_LEFT_2=149,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=59.719
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.50
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3750
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=0.4059
PRIMER_LEFT_3_PENALTY=0.125045
PRIMER_RIGHT_3_PENALTY=0.280862
PRIMER_LEFT_3_SEQUENCE=AACCAACNTCACACTTGAAAGC
PRIMER_RIGHT_3_SEQUENCE=CAGTGGTAGTCTAACCGACACG
PRIMER_LEFT_3=159,22
PRIMER_RIGHT_3=288,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=59.719
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=130
PRIMER_PAIR_PENALTY_4=0.4392
PRIMER_LEFT_4_PENALTY=0.158297
PRIMER_RIGHT_4_PENALTY=0.280862
PRIMER_LEFT_4_SEQUENCE=ATCGGTACTGCTTCGTGAGAAT
PRIMER_RIGHT_4_SEQUENCE=CAGTGGTAGTCTAACCGACACG
PRIMER_LEFT_4=87,22
PRIMER_RIGHT_4=288,22
PRIMER_LEFT_4_TM=60.158
PRIMER_RIGHT_4_TM=59.719
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_04028 Ps_contig_mira-and-tgicl-ass_franssen_31875
SEQUENCE=CAAAACTNGGATTTTCTTCTTTTATTTATTTATTTCTTCTTCCTTTCCCACCCCCTCTCTCTCTCTCTCAGCTTTCAAGTGTGATGTTGGTTTCTGTGCTTTTCTTGGACCAAATTTCCTCTACTCAGAGGTGAAGGAAATGATTCTCACGAAGCAGTACCGATGCGTACACTCGTCTTCTTGCCAATGTACAAAAGGTCATTTAAGCGAAGATGTGATATTCTTGGTGTTTAACAATTTGAATTGGAACCCGAAGCTAATAGCTACTCTCTCATGTGTATGCAAATGGTTCGATGATCTTGCCAAGCGAGTTTTATGGAAAGAGTTTTGTCGAACGAGAGCTCCGAAGATGATGATTGATCTCCAATCTAGCGGAACTCACAGTATTGATGGAAACTGGAGTGCCTTAGGGAAGCTGCTTATATACTGTTCGGGATGCAAAAAAGGCGGTTTGTTCAAGAACGTGCATATCCCCGGTCATTTCGTTTACAGGACACGATTCTCTAAAACATCGGGAAAGAGCTTTTACTTTTAGACCGACAATGTATAAATGATGTTTTGTATGT
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04029 Ps_contig_mira-and-tgicl-ass_franssen_31875
SEQUENCE=CAAAACTNGGATTTTCTTCTTTTATTTATTTATTTCTTCTTCCTTTCCCACCCCCTCTCTCTCTCTCTCAGCTTTCAAGTGTGATGTTGGTTTCTGTGCTTTTCTTGGACCAAATTTCCTCTACTCAGAGGTGAAGGAAATGATTCTCACGAAGCAGTACCGATGCGTACACTCGTCTTCTTGCCAATGTACAAAAGGTCATTTAAGCGAAGATGTGATATTCTTGGTGTTTAACAATTTGAATTGGAACCCGAAGCTAATAGCTACTCTCTCATGTGTATGCAAATGGTTCGATGATCTTGCCAAGCGAGTTTTATGGAAAGAGTTTTGTCGAACGAGAGCTCCGAAGATGATGATTGATCTCCAATCTAGCGGAACTCACAGTATTGATGGAAACTGGAGTGCCTTAGGGAAGCTGCTTATATACTGTTCGGGATGCAAAAAAGGCGGTTTGTTCAAGAACGTGCATATCCCCGGTCATTTCGTTTACAGGACACGATTCTCTAAAACATCGGGAAAGAGCTTTTACTTTTAGACCGACAATGTATAAATGATGTTTTGTATGT
TARGET=55,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4076
PRIMER_LEFT_PENALTY=1.375269
PRIMER_RIGHT_PENALTY=0.032347
PRIMER_LEFT_SEQUENCE=TATTTCTTCTTCCTTTCCCACC
PRIMER_RIGHT_SEQUENCE=TGACCTTTTGTACATTGGCAAG
PRIMER_LEFT=30,22
PRIMER_RIGHT=200,22
PRIMER_LEFT_TM=58.625
PRIMER_RIGHT_TM=60.032
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=1.5336
PRIMER_LEFT_1_PENALTY=1.375269
PRIMER_RIGHT_1_PENALTY=0.158297
PRIMER_LEFT_1_SEQUENCE=TATTTCTTCTTCCTTTCCCACC
PRIMER_RIGHT_1_SEQUENCE=ATCGGTACTGCTTCGTGAGAAT
PRIMER_LEFT_1=30,22
PRIMER_RIGHT_1=163,22
PRIMER_LEFT_1_TM=58.625
PRIMER_RIGHT_1_TM=60.158
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=1.6444
PRIMER_LEFT_2_PENALTY=1.375269
PRIMER_RIGHT_2_PENALTY=0.269153
PRIMER_LEFT_2_SEQUENCE=TATTTCTTCTTCCTTTCCCACC
PRIMER_RIGHT_2_SEQUENCE=GCTTCGTGAGAATCATTTCCTT
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=58.625
PRIMER_RIGHT_2_TM=59.731
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=1.6801
PRIMER_LEFT_3_PENALTY=1.375269
PRIMER_RIGHT_3_PENALTY=0.304841
PRIMER_LEFT_3_SEQUENCE=TATTTCTTCTTCCTTTCCCACC
PRIMER_RIGHT_3_SEQUENCE=TACATTGGCAAGAAGACGAGTG
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=190,22
PRIMER_LEFT_3_TM=58.625
PRIMER_RIGHT_3_TM=60.305
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=1.6801
PRIMER_LEFT_4_PENALTY=1.375269
PRIMER_RIGHT_4_PENALTY=0.304841
PRIMER_LEFT_4_SEQUENCE=TATTTCTTCTTCCTTTCCCACC
PRIMER_RIGHT_4_SEQUENCE=CATTGGCAAGAAGACGAGTGTA
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=188,22
PRIMER_LEFT_4_TM=58.625
PRIMER_RIGHT_4_TM=60.305
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_04030 Ps_contig_mira-and-tgicl-ass_franssen_31877
SEQUENCE=TTCTTAGTACCTGCTTTCAATCCCTGCCAAGGAAGGCTTCCTCTGAGGAAGTACAAAAGCACATACCCCAGTGACTCCAAATCATCCCGCCGGCTTTGCTCAATCCCAAGATGAGTATTGCAGCTTGCATACCGTGCAGTACCTGTTAAGTTTTTGTTCTCCCTGTAGGGGATGTGGCGATTGGTTGCAGAGTCCCGATATCGTTTTGCAAGCCCAAAATCAATTACATAAACCAGGTTTGCCTTCCGACCAAGTCCCATGAGAAAGTTATCCGGTTTAATATCCCTGTGTAGGAATCCTTTAGAATGCACGTACTCTATTCTGGTCATCATTTGATCAGCCAACATCAAGACAGTTTTGAGAGAGAACTTCCTCCCACAGTAAACAAAAAGATCCTCTAGACTAGGCCCCAACAAATCCATAACAAGCACATTGTCTTCTCCGTCTACACCACTCCATCTAATGCTTGGGATACCACTTCCTCCTTGAAGAATATTATAGAGCTTGGCTTCATAAAGCAATTGTGGATGCTTCGTTTTACCAGTCTCGATCTTGACGGCAACGATCTCGAACGTATCAATATGAGTAGCGAGATAGATTTCACCGAAGGATCCGCTTCCGATCTTTCGACCTAGCTTGAACTTCTCGCCGATGATTCGATCCATTATCGGTATTCTGAATGAATCTGATGATAGTATATCTCTTACAAGCAGAATAAAACCCTAAACTATAGTTACATAGATAGATACAGGATAATAATAATACAACAAGGGGAAGAAGAAGAAGAAGAAAGAAGGTAACGCTAATACGAGTAGCCGGTAGCCACCGCAAATGGAAGTATGAATTCAACACGCGGACTCACTTTTGTCTTCTTTATCAATCTTCTTATTACATTCTTCTTCCTCCCTCCCCCCGTATCTG
TARGET=753,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0838
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.071684
PRIMER_LEFT_SEQUENCE=GATGATTCGATCCATTATCGGT
PRIMER_RIGHT_SEQUENCE=AAGAAGACAAAAGTGAGTCCGC
PRIMER_LEFT=650,22
PRIMER_RIGHT=873,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=59.928
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.1943
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.071684
PRIMER_LEFT_1_SEQUENCE=AGCGAGATAGATTTCACCGAAG
PRIMER_RIGHT_1_SEQUENCE=AAGAAGACAAAAGTGAGTCCGC
PRIMER_LEFT_1=587,22
PRIMER_RIGHT_1=873,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.1982
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=0.186036
PRIMER_LEFT_2_SEQUENCE=GATGATTCGATCCATTATCGGT
PRIMER_RIGHT_2_SEQUENCE=TACCGGCTACTCGTATTAGCGT
PRIMER_LEFT_2=650,22
PRIMER_RIGHT_2=820,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.186
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.3086
PRIMER_LEFT_3_PENALTY=0.122569
PRIMER_RIGHT_3_PENALTY=0.186036
PRIMER_LEFT_3_SEQUENCE=AGCGAGATAGATTTCACCGAAG
PRIMER_RIGHT_3_SEQUENCE=TACCGGCTACTCGTATTAGCGT
PRIMER_LEFT_3=587,22
PRIMER_RIGHT_3=820,22
PRIMER_LEFT_3_TM=59.877
PRIMER_RIGHT_3_TM=60.186
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.3969
PRIMER_LEFT_4_PENALTY=0.210886
PRIMER_RIGHT_4_PENALTY=0.186036
PRIMER_LEFT_4_SEQUENCE=GCTTCGTTTTACCAGTCTCGAT
PRIMER_RIGHT_4_SEQUENCE=TACCGGCTACTCGTATTAGCGT
PRIMER_LEFT_4=529,22
PRIMER_RIGHT_4=820,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=60.186
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.3000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_04031 Ps_contig_mira-and-tgicl-ass_franssen_31877
SEQUENCE=TTCTTAGTACCTGCTTTCAATCCCTGCCAAGGAAGGCTTCCTCTGAGGAAGTACAAAAGCACATACCCCAGTGACTCCAAATCATCCCGCCGGCTTTGCTCAATCCCAAGATGAGTATTGCAGCTTGCATACCGTGCAGTACCTGTTAAGTTTTTGTTCTCCCTGTAGGGGATGTGGCGATTGGTTGCAGAGTCCCGATATCGTTTTGCAAGCCCAAAATCAATTACATAAACCAGGTTTGCCTTCCGACCAAGTCCCATGAGAAAGTTATCCGGTTTAATATCCCTGTGTAGGAATCCTTTAGAATGCACGTACTCTATTCTGGTCATCATTTGATCAGCCAACATCAAGACAGTTTTGAGAGAGAACTTCCTCCCACAGTAAACAAAAAGATCCTCTAGACTAGGCCCCAACAAATCCATAACAAGCACATTGTCTTCTCCGTCTACACCACTCCATCTAATGCTTGGGATACCACTTCCTCCTTGAAGAATATTATAGAGCTTGGCTTCATAAAGCAATTGTGGATGCTTCGTTTTACCAGTCTCGATCTTGACGGCAACGATCTCGAACGTATCAATATGAGTAGCGAGATAGATTTCACCGAAGGATCCGCTTCCGATCTTTCGACCTAGCTTGAACTTCTCGCCGATGATTCGATCCATTATCGGTATTCTGAATGAATCTGATGATAGTATATCTCTTACAAGCAGAATAAAACCCTAAACTATAGTTACATAGATAGATACAGGATAATAATAATACAACAAGGGGAAGAAGAAGAAGAAGAAAGAAGGTAACGCTAATACGAGTAGCCGGTAGCCACCGCAAATGGAAGTATGAATTCAACACGCGGACTCACTTTTGTCTTCTTTATCAATCTTCTTATTACATTCTTCTTCCTCCCTCCCCCCGTATCTG
TARGET=774,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0838
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.071684
PRIMER_LEFT_SEQUENCE=GATGATTCGATCCATTATCGGT
PRIMER_RIGHT_SEQUENCE=AAGAAGACAAAAGTGAGTCCGC
PRIMER_LEFT=650,22
PRIMER_RIGHT=873,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=59.928
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.1943
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.071684
PRIMER_LEFT_1_SEQUENCE=AGCGAGATAGATTTCACCGAAG
PRIMER_RIGHT_1_SEQUENCE=AAGAAGACAAAAGTGAGTCCGC
PRIMER_LEFT_1=587,22
PRIMER_RIGHT_1=873,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.1982
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=0.186036
PRIMER_LEFT_2_SEQUENCE=GATGATTCGATCCATTATCGGT
PRIMER_RIGHT_2_SEQUENCE=TACCGGCTACTCGTATTAGCGT
PRIMER_LEFT_2=650,22
PRIMER_RIGHT_2=820,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.186
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.3086
PRIMER_LEFT_3_PENALTY=0.122569
PRIMER_RIGHT_3_PENALTY=0.186036
PRIMER_LEFT_3_SEQUENCE=AGCGAGATAGATTTCACCGAAG
PRIMER_RIGHT_3_SEQUENCE=TACCGGCTACTCGTATTAGCGT
PRIMER_LEFT_3=587,22
PRIMER_RIGHT_3=820,22
PRIMER_LEFT_3_TM=59.877
PRIMER_RIGHT_3_TM=60.186
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.3969
PRIMER_LEFT_4_PENALTY=0.210886
PRIMER_RIGHT_4_PENALTY=0.186036
PRIMER_LEFT_4_SEQUENCE=GCTTCGTTTTACCAGTCTCGAT
PRIMER_RIGHT_4_SEQUENCE=TACCGGCTACTCGTATTAGCGT
PRIMER_LEFT_4=529,22
PRIMER_RIGHT_4=820,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=60.186
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.3000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_04032 Ps_contig_mira-and-tgicl-ass_franssen_31878
SEQUENCE=AAAACANAATTATTTAAAATAATTATTTCATATATGTTTTGTCATATATCATATAATCAAATCAGTATACCAGTCATAGACATGGATTAGTTTAGAACTGGCATGTAGACATGAAAGAAGTTCTAATTGATATCCATAGTCAAACACAAACCAAAGTATCACAACTCCCAAATATAGAGTGGGTACATTTAGTAAAGTAAAACAGAACAGTTGAAGATCATCATAGTGCACACAACTTAATTAAGCTTAAGAGCTTGAGCATGATGCCGAATGTGGTCATCAATGAAGGTGGCAATGAAATAGTAGGAGTGATCATAACCAGGTTGAAGACGTAACAACAGTGGAACGTTGCCATTCTTGCAGGCCTCTTCAAACTTGTGAGGCAACAGTTGATCATGCAAAAATTTGTCACCTTCCCCCTGATCAATCAAAATGGTGGTAGATACATTGGGATACTTTTTGACCAGATAAGTGGCGTCGTAATCCTCCCAATCAGATTTATTGCCACCTAGATAATTAGTGAATGCCTTCTGTCCCCAAGGACAATTTGTAGGATTTGCTATTGGTGCAAAGGCAGAAACAGACTTATATTTATCCAGGTTTTTCAGGTAGATAGTCAGTGCACCATGCCCACCCATAGAATGGCCAAATATAGAAGCTTTTGATGTGTCAAGCTGTGGAAAGTTATCACGTAGAAGCTCGGGCAATTCCTTGACAACATAGTCATACATGCGCCAATTCTTCCATTTCTCTTGTGTAGCGTTGAGATAAAATCCTGCACCTACACCAAAATCCCATGAATCTGCTTCTCCTTCAACATTCAAGCCTCTTGGAGAAGTGTCGGGAGCGATCAGGGCAACGCCGTGTTGGGAAGCGGCGCGTTGAGCATCGGATTTGAAAATGAAATTCTCATCGGTGCAAGAGAGGCCAGATAGCCAGTAGAGAACGGGGAATTTGTGGGAAACCGTGGAATCAGAAGGTGGGAAGTATATGTGGAAGTTCATGGAACATCCAAGGGTATGGCTGTAATGTTTGTATCTCTTATTGTAACCTCCATGCATCTTCGCACTACTCGTCTCCGTCACTCCCCCCTCCATTTTCGTCTTCTTCTTCTTTCTCTCTCTACAAACTCACTCTCTCTAACTAAGACTGAATGCCCCCGACTCTGTCGTTTGTATACCACTGCTT
TARGET=1103,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8491
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.844757
PRIMER_LEFT_SEQUENCE=GAGAGGCCAGATAGCCAGTAGA
PRIMER_RIGHT_SEQUENCE=GGGGGCATTCAGTCTTAGTTAG
PRIMER_LEFT=921,22
PRIMER_RIGHT=1160,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.155
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.8491
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.844757
PRIMER_LEFT_1_SEQUENCE=GAGGCCAGATAGCCAGTAGAGA
PRIMER_RIGHT_1_SEQUENCE=GGGGGCATTCAGTCTTAGTTAG
PRIMER_LEFT_1=923,22
PRIMER_RIGHT_1=1160,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.155
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.9402
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.844757
PRIMER_LEFT_2_SEQUENCE=ATCCAAGGGTATGGCTGTAATG
PRIMER_RIGHT_2_SEQUENCE=GGGGGCATTCAGTCTTAGTTAG
PRIMER_LEFT_2=1009,22
PRIMER_RIGHT_2=1160,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=59.155
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.9402
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.844757
PRIMER_LEFT_3_SEQUENCE=CATCCAAGGGTATGGCTGTAAT
PRIMER_RIGHT_3_SEQUENCE=GGGGGCATTCAGTCTTAGTTAG
PRIMER_LEFT_3=1008,22
PRIMER_RIGHT_3=1160,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=59.155
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=153
PRIMER_PAIR_PENALTY_4=0.9739
PRIMER_LEFT_4_PENALTY=0.129103
PRIMER_RIGHT_4_PENALTY=0.844757
PRIMER_LEFT_4_SEQUENCE=CCAAGGGTATGGCTGTAATGTT
PRIMER_RIGHT_4_SEQUENCE=GGGGGCATTCAGTCTTAGTTAG
PRIMER_LEFT_4=1011,22
PRIMER_RIGHT_4=1160,22
PRIMER_LEFT_4_TM=60.129
PRIMER_RIGHT_4_TM=59.155
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_04033 Ps_contig_mira-and-tgicl-ass_franssen_31881
SEQUENCE=TTTACTGTTCCAGAGCTTGATGATTCAACACCGCTTYTNCGGCAAGAGGGTGTTGAAGAGTTCGGTTTTGTTGGAAGTGAGAGCAAGGGTTTGGAACAGAGTGGTAAAATTAGTTGTAAAGTTGAGGAACAAGTGAGTGAGATTCCTGTTTTTGATATGGGTTTGTGTGGTGGTGGTGGTGGTGGTGGTCAGAAGAAAGGTAAATCCAAAAGGGTTGAAGGACAACCTTCCAAGAATCTCATGGCGGAAAGAAGGAGGAGAAAGAGGTTGAATGATAGGCTTTCCATGCTTAGGTCAATTGTCCCTAAGATCAGTAAGATGGACAGGACATCGATTCTTGGGGATACAATTGATTACATGAAAGAGCTTCTTGAAAGGATCGGAAAGTTGCAAGAAGAAATGGATGAAGGAACAGATCAGATAAATGTTTTGGGAATTTCTAAGGAGTTAAAGCCAAATGAAGTCATGGTAAGAAATTCCCCTAAGTTTAATGTTGAGAGGAGAGATCAAGACACAAGGATTAGCATCTGCTGTGCCACAAAGCCAGGTTTATTACTGTCAACAGTGAACACATTAGAAGCATTAGGCCTTGAGATTCACCAATGTGTTATAAGCAGTTTCAATGATTTTTCATTGCAAGCATCTTGCTCAGAGGTCGCTGGACAGAGAAATTGTATGAACCCTGAAGAGATAAAGCAATCACTGTTCAGGAATGCAGGTTATGGAGGGAGGTGCCTCTAGGAAAAGGATGGAAAGCTGACATAACAATTTGCAGATGGTGAAAATGAGACAAAATTTTCCTCTTATGTAATTCAAGTAATTAGGGTAGTTAAAAAGTTAGTAATGATANTCCAAATAAATTGGCTTTTGCTGATGCAATCACTCTTAGGGTTATACCCTGTATTTAAACG
TARGET=167,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04034 Ps_contig_mira-and-tgicl-ass_franssen_31895
SEQUENCE=CACACTTCGAAACACATGTTTGGACAGATCTACATCCCGGAAATAAACTGGATTACTCATGGTTCTGACTCTCGCTCGTAACCATTGGATTTCAAGACACAACCTTAATCGGAAACGCTTACGGACTTGCTTGTATGACCGTAATGTTTGTGACGACATTTCTGATGGCACTAGTCATAGTTTTCGTCTGGCAGAAAAGCTTCGTGATTGCTGCACTATTCCTTTTGTTCTTTTGGGTGATAGAAGCGGTATATCTATCGGCGGCGTTCCTAAAAGTGCACCAAGGAGGATGGGTTCCGCTTGTCCTGTCGTTCTTCTTTTTGGTCGTTATGTATGTATGGCATTACGGTATTCGTAGGAAGTACAAATACGATCTACACAACAAAGTTTCGTTGAAATGGTTACTAGGTTTGGGACCTAGTCTTGGAATTGTCCGTGTCCCGGGGATCGGTCTCATCTACTCGGAACTCGCAACGGGAATACCAGCTATATTCACTCATTTCGTAACTAACCTCCCTGCATTTCACAAGGTTTTGGTTTTCGTCTGCGTAAAATCGGTTCCTGTTCCACATGTCTCGCCTGAAGAACGGTTTCTGATTGGGCGAGCTTGTCCAAGACCGTTTCGAATGTATAGGTGCATTGTTCGATACGGCTACAAAGACATTAAAAGGGACGACGGCGAGTTCGAGAACCATCTCATACAAAGTATAATGGAGTTCATCCAAATGGAAGCGGTAGAACCACAGTTCTCAAGTTCCGATTCTTCGTCATTTGACGTGAGAATGGCTGTTATAGGCACCAGAAGCTTAGAATCAACTCCAACCTTGATCGCGTCCGAGCAAGACGACGTTGATATCGACGAAAGCATCCCTAGTAGCCGGTCTATAACACTACGACGCCTACAATCCACCATCGACGACGAGAACCCGCAAGTCAGAAAACGTCGAGTAAGGTTTCAAGTGCCTAACAATCCCGGGTTGGATAATGCGGTTAAAGAAGAGATTCTAGACTTAATCCAAGCCAAGGAAGCGGGAATCGCGTATATAATGGGACACTCATATGTGAAGGCGAGGAAATCATCGTCGTTCTTGAAAAAGATTGTGATCGATATGGGATACTCTTTTCTGCGGAAGAATTGCAGGGGTCCGGCTGTGGCTCTTAACATTCCACACATCAGTCTCATTGAAGTTGGAATGATATATTATGTTTAGTTTGTGGAATTTTACCATTTAGGTTATAGAGACTCTTAGTAATGTGTATCTTGCTGTGAGTCTTAAACTGAAAAATGTGAAATTTCTATTAACTTAATCGATTGTTTTAAGGTTCTTAAAGCAAGTTGGATACAAGGTTTTCATTTTACATGATTGAACAAAATGTTTGGAGAATGATTGAAAGAAGAAAA
TARGET=329,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0929
PRIMER_LEFT_PENALTY=0.071684
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=GCTTGTCCTGTCGTTCTTCTTT
PRIMER_RIGHT_SEQUENCE=AATGCAGGGAGGTTAGTTACGA
PRIMER_LEFT=298,22
PRIMER_RIGHT=522,22
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.1503
PRIMER_LEFT_1_PENALTY=0.129103
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=GTTCTTTTGGGTGATAGAAGCG
PRIMER_RIGHT_1_SEQUENCE=AATGCAGGGAGGTTAGTTACGA
PRIMER_LEFT_1=226,22
PRIMER_RIGHT_1=522,22
PRIMER_LEFT_1_TM=60.129
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.1573
PRIMER_LEFT_2_PENALTY=0.136083
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=TTTTGGGTGATAGAAGCGGTAT
PRIMER_RIGHT_2_SEQUENCE=AATGCAGGGAGGTTAGTTACGA
PRIMER_LEFT_2=230,22
PRIMER_RIGHT_2=522,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.8000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.1652
PRIMER_LEFT_3_PENALTY=0.143968
PRIMER_RIGHT_3_PENALTY=0.021195
PRIMER_LEFT_3_SEQUENCE=GTCCTGTCGTTCTTCTTTTTGG
PRIMER_RIGHT_3_SEQUENCE=AATGCAGGGAGGTTAGTTACGA
PRIMER_LEFT_3=302,22
PRIMER_RIGHT_3=522,22
PRIMER_LEFT_3_TM=60.144
PRIMER_RIGHT_3_TM=60.021
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.1652
PRIMER_LEFT_4_PENALTY=0.143968
PRIMER_RIGHT_4_PENALTY=0.021195
PRIMER_LEFT_4_SEQUENCE=TCCTGTCGTTCTTCTTTTTGGT
PRIMER_RIGHT_4_SEQUENCE=AATGCAGGGAGGTTAGTTACGA
PRIMER_LEFT_4=303,22
PRIMER_RIGHT_4=522,22
PRIMER_LEFT_4_TM=60.144
PRIMER_RIGHT_4_TM=60.021
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_04035 Ps_contig_mira-and-tgicl-ass_franssen_31898
SEQUENCE=AGTGAGACTTGTATTTAGCGGTGGAATCAAAAGTGAGGCAACCGTCTCAACNCCACNAAAAATACCATTTCCAATAATACATATTTATATTCATATTATTTTCAACAACTCCATTCTCAAAGCTCACAACAACAACCACCACCTCAATTTCCGATCCACTTTCATGTTTTTTCCAATCCTAAATTGAAGTTGAGAGTCGAAAAAGAACATGATGTTGTGTTTGGGTGTAGTTGGTGGGTCCACAAAATGTCTTCAACTCTATGATTCCAGTTACAGAATCAACAACAAAAGATTCCTTAATCTAAACAACAACAACGGTTTCAGTTTCAATACGAGGTGGCGTTTCATGGCACTTGAATCGGACTCTTCGTCTTTTGCATCGTCTATTGATTCTGACACCACCGATAAAAACGCTGCTGCTACTGGGTTCTGCATTATAGAAGGACCTGAAACTGTACAAGATTTTGCTAAAATGGAACTTCAAGAAATTCAGGATAATATTCGAAGTCGAAGGAATAAGATTTTCTTACATATGGAAGAGGTTCGTAGGCTTAGAATACAACAGAGGATTAAAAATGCTGAACTTGGAATTTTGCAAGAAGAGCAAGAGAATGAACTTCCGAACTTCCCATCATTCATTCCATTCTTGCCTCCTTTGACTTCAGCGAACCTCAAGCAGTATTATGCAACTTGTTTTTCTCTTATCGGTGGGATCATTCTCTTTGGTGGCCTTCTTGCTCCGAGTTTGGAGCTTAAGCTTGGAATAGGAGGCACATCTTATGCCGATTTTATTCAAAACGTGCATCTGCCTATGCAGTTGAGTCAGGTTGATCCTATTGTGGCATCATTCTCAGGTGGAGCGGTTGGAGTAATCTCTGCATTGATGGTTGTTGAGATAAACAATGTAAAACAACAGGAGCAGAAAAGATGCAAGTACTGTCTTGGTACTGGATATCTTGCATGCGCTCGCTGTTCAAGCACGGGTGCACTTGTTCTGATTGAACCGGTATCCACCTTCAATGGTGCAGATCAGCCTCTATCACCACCGAAAACCGAGAGATGTTCAAACTGCTCCGGATCTGGCAAGGTCATGTGTCCAACATGTCTTTGCACTGGAATGGCCATGGCAAGTGAACATGATCCTCGAATTGATCCTTTTGACTAGAGATTTAATATTATTGTGGTAGATATCCTAAGTAGGGAATTTGAAATTTTATGATGAGTATGGTATGTGTTTCTGTTGTGAAATAATGGAATTTCTGTAAATATATATGAACACAATAATATCCAAAAGAAAATGGTTATGGATCTAGACAGTTTCCCTTATTTTTCAGAATTGAATGTGTTGCAGCAGGAATTTTTATTG
TARGET=305,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0651
PRIMER_LEFT_PENALTY=0.058146
PRIMER_RIGHT_PENALTY=0.006945
PRIMER_LEFT_SEQUENCE=ACATGATGTTGTGTTTGGGTGT
PRIMER_RIGHT_SEQUENCE=TCGGTGGTGTCAGAATCAATAG
PRIMER_LEFT=206,22
PRIMER_RIGHT=404,22
PRIMER_LEFT_TM=60.058
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.0760
PRIMER_LEFT_1_PENALTY=0.058146
PRIMER_RIGHT_1_PENALTY=0.017846
PRIMER_LEFT_1_SEQUENCE=ACATGATGTTGTGTTTGGGTGT
PRIMER_RIGHT_1_SEQUENCE=TAGACGATGCAAAAGACGAAGA
PRIMER_LEFT_1=206,22
PRIMER_RIGHT_1=385,22
PRIMER_LEFT_1_TM=60.058
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.1726
PRIMER_LEFT_2_PENALTY=0.058146
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=ACATGATGTTGTGTTTGGGTGT
PRIMER_RIGHT_2_SEQUENCE=AAGACGAAGAGTCCGATTCAAG
PRIMER_LEFT_2=206,22
PRIMER_RIGHT_2=373,22
PRIMER_LEFT_2_TM=60.058
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.1877
PRIMER_LEFT_3_PENALTY=0.180765
PRIMER_RIGHT_3_PENALTY=0.006945
PRIMER_LEFT_3_SEQUENCE=CTCAAAGCTCACAACAACAACC
PRIMER_RIGHT_3_SEQUENCE=TCGGTGGTGTCAGAATCAATAG
PRIMER_LEFT_3=115,22
PRIMER_RIGHT_3=404,22
PRIMER_LEFT_3_TM=59.819
PRIMER_RIGHT_3_TM=59.993
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.1986
PRIMER_LEFT_4_PENALTY=0.180765
PRIMER_RIGHT_4_PENALTY=0.017846
PRIMER_LEFT_4_SEQUENCE=CTCAAAGCTCACAACAACAACC
PRIMER_RIGHT_4_SEQUENCE=TAGACGATGCAAAAGACGAAGA
PRIMER_LEFT_4=115,22
PRIMER_RIGHT_4=385,22
PRIMER_LEFT_4_TM=59.819
PRIMER_RIGHT_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_04036 Ps_contig_mira-and-tgicl-ass_franssen_31901
SEQUENCE=GTTNGCAAGAATAATAATAATAATATTACTCTATCAATTCATATAATGTAACTAGTGTATATATATACATATACTACAAAGCAATATAATGCATATTTAGAACTCAAAAATGATCTTCCCAGTTGCATGGCCATCAATACTCTTAGCCCAACCATCTTCAGCTTTTGTTATAGGATATTTTGAGTCAATAACTGTTCTAAGTTTTCCTTGCTTAACCAAATCAACAAGATATTGAAGATCCTTACCCTTTGGAACCAAAAGCAATGGCACAAGTTGTTTTCTAGAGAAGGTAAGTTTCTTAAGTGCAAATGTCATCATAGAACTAGAGCTAGGAGTTATGTCTACAACCTTACCATTCGTGCTCAAGTTAGGCTCAAAAGTTGACCATGGAACTCCTACTGCACAATGAATCACTGCATCATATTTCTTACCAGATGGACTCTTTAATGCAGCACCATCAGATGTCTTATAATCAATCACCTCATCAGCACCTAAGCTTTTGACAAATTCGATGTTTCGAGCGCCGCAGGTGGCCGTCACATGAGTGTTGCCTAGTTTGGCTAATTGAACTGCATAGTGGCCTACACCCCCTGATGCAGCAGTGACCAAAATGTTTTTCCGTTCACCGCTTCCATCGAGTTTGATTCCTATTGATTGGGAAAGTGCTTGGAGAGCTGTTAGACCTGCAACAGGTAAGCCGGCGCATTCGGATGCTGTGATTTCTGGTGGTCTTGAAGCTGTGAGACACTCCTTAACAATAGCAAACTCAGCTAGTCCTCCACCACTAAAGGGGCTGACGAAGCCTACAACTTTGTCACCGGCTTTGAACTTTTTAACACCTTTTCCAACCTCCATGACTTCTCCTGCTATATCAGTACAAGGTATATATGGGAATTTGGGAGGCAAAATAGGCCACAGCATGCGTTTCTGTACTTTCCAATCAAATGGGTTTATGCTAGCTGCTTCCAATTTTATCAAAACCTCATCTTTACTAGGACTCGGTATCGGAACTTCAACATGCTTTAAGGCATTGGATCCTCCACCATAGGCATTGTACTGAACAGCACGCATGAGTTTCTTCTCCATCTACAAAGCGAGAGCGTGTTGAGAATGTGTTTCCATCTAAAAAAGTTGAAACGTAGTTAAGAAACGTGTGGATAGAAACAAGACNAGTAGTGGCGTCCAAGGATGCATAGGTTTTGCCACCCCGCCAAT
TARGET=10,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04037 Ps_contig_mira-and-tgicl-ass_franssen_31901
SEQUENCE=GTTNGCAAGAATAATAATAATAATATTACTCTATCAATTCATATAATGTAACTAGTGTATATATATACATATACTACAAAGCAATATAATGCATATTTAGAACTCAAAAATGATCTTCCCAGTTGCATGGCCATCAATACTCTTAGCCCAACCATCTTCAGCTTTTGTTATAGGATATTTTGAGTCAATAACTGTTCTAAGTTTTCCTTGCTTAACCAAATCAACAAGATATTGAAGATCCTTACCCTTTGGAACCAAAAGCAATGGCACAAGTTGTTTTCTAGAGAAGGTAAGTTTCTTAAGTGCAAATGTCATCATAGAACTAGAGCTAGGAGTTATGTCTACAACCTTACCATTCGTGCTCAAGTTAGGCTCAAAAGTTGACCATGGAACTCCTACTGCACAATGAATCACTGCATCATATTTCTTACCAGATGGACTCTTTAATGCAGCACCATCAGATGTCTTATAATCAATCACCTCATCAGCACCTAAGCTTTTGACAAATTCGATGTTTCGAGCGCCGCAGGTGGCCGTCACATGAGTGTTGCCTAGTTTGGCTAATTGAACTGCATAGTGGCCTACACCCCCTGATGCAGCAGTGACCAAAATGTTTTTCCGTTCACCGCTTCCATCGAGTTTGATTCCTATTGATTGGGAAAGTGCTTGGAGAGCTGTTAGACCTGCAACAGGTAAGCCGGCGCATTCGGATGCTGTGATTTCTGGTGGTCTTGAAGCTGTGAGACACTCCTTAACAATAGCAAACTCAGCTAGTCCTCCACCACTAAAGGGGCTGACGAAGCCTACAACTTTGTCACCGGCTTTGAACTTTTTAACACCTTTTCCAACCTCCATGACTTCTCCTGCTATATCAGTACAAGGTATATATGGGAATTTGGGAGGCAAAATAGGCCACAGCATGCGTTTCTGTACTTTCCAATCAAATGGGTTTATGCTAGCTGCTTCCAATTTTATCAAAACCTCATCTTTACTAGGACTCGGTATCGGAACTTCAACATGCTTTAAGGCATTGGATCCTCCACCATAGGCATTGTACTGAACAGCACGCATGAGTTTCTTCTCCATCTACAAAGCGAGAGCGTGTTGAGAATGTGTTTCCATCTAAAAAAGTTGAAACGTAGTTAAGAAACGTGTGGATAGAAACAAGACNAGTAGTGGCGTCCAAGGATGCATAGGTTTTGCCACCCCGCCAAT
TARGET=58,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04038 Ps_contig_mira-and-tgicl-ass_franssen_31908
SEQUENCE=AGAAGCACAAAACAAAAAGGGAATGAGAGTGGCATTGGAGAAAGAAAGAACTCAAGGGATGAGGATGGCCGTGGAGAGAAAAAGAAGCTCTAGGGATGGGGATGATCATAGAGAAACAAAAATCAGAAGGATTGAGGATGACCGTACGGGAAGAAAGGCCATAAGGAATGGAGATGATTATGGAGAAAGAAATCACTCGAGGAATGAGGGCCGCCATAGAGAAGCACCAAACAAAAGGGATGGGGCTGGCAGTGGAGAAGTAAAGAACTCAAGAGATGAGGATGATCGCAAAGAAATAAAGAACTCAAGGGGTGAGGATGATTACAAGGAGAGAAAGAGCTCCAGGGATGAGGATCTTCGTGAGGAAAGAAGAGAAGCACCAAACAGAAGGGACGGGGCTGGCAGTGGAGAAGTTAAGAACTCAAGGAACGAGGAAGATCGCAGAGAAAGAAAGAACTCAAGGGATGAGGATGATTATAAGGAGAGAAGGAGCTCTAGGGATGAGGATGTGCGTGAGGAAAGAAAGCACAGAGGGGGTGAGGATTATCATGGAGAAAGAAAGCGCAGAAGGGATGATGATGATCATGTGCATGGAGAAAGAAGGCATTATAGAAGGGATGATGATGATGGAGAAAGAAAGTACAGAAGGGAGTAGTTTGAGCAGGAAAATGAAGTTTATAAGAGGGATATCCATGTGAATTACTCCCAGAAAACCAAATTCAATGTATCTAGTTCAAGGGAGAGCATGAAAACCCAATACCGTAGTCCAAGGATTTAGAATGCTGATGTTGTGCTTTAGCCTTTGAATGTGTCTTTTAGTTCTTTTTTTAGTTGTTCTTGTCGTTTTCTGCTTAGTTTAAAAGCGTTTTAG
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6176
PRIMER_LEFT_PENALTY=0.500551
PRIMER_RIGHT_PENALTY=0.117097
PRIMER_LEFT_SEQUENCE=AACAAAAAGGGAATGAGAGTGG
PRIMER_RIGHT_SEQUENCE=TTCTCTATGATCATCCCCATCC
PRIMER_LEFT=10,22
PRIMER_RIGHT=114,22
PRIMER_LEFT_TM=59.499
PRIMER_RIGHT_TM=60.117
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=105
PRIMER_PAIR_PENALTY_1=0.6739
PRIMER_LEFT_1_PENALTY=0.500551
PRIMER_RIGHT_1_PENALTY=0.173354
PRIMER_LEFT_1_SEQUENCE=AACAAAAAGGGAATGAGAGTGG
PRIMER_RIGHT_1_SEQUENCE=TCCTCGAGTGATTTCTTTCTCC
PRIMER_LEFT_1=10,22
PRIMER_RIGHT_1=202,22
PRIMER_LEFT_1_TM=59.499
PRIMER_RIGHT_1_TM=59.827
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.7877
PRIMER_LEFT_2_PENALTY=0.500551
PRIMER_RIGHT_2_PENALTY=0.287182
PRIMER_LEFT_2_SEQUENCE=AACAAAAAGGGAATGAGAGTGG
PRIMER_RIGHT_2_SEQUENCE=CCTCATTCCTCGAGTGATTTCT
PRIMER_LEFT_2=10,22
PRIMER_RIGHT_2=208,22
PRIMER_LEFT_2_TM=59.499
PRIMER_RIGHT_2_TM=59.713
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=0.9913
PRIMER_LEFT_3_PENALTY=0.500551
PRIMER_RIGHT_3_PENALTY=0.490741
PRIMER_LEFT_3_SEQUENCE=AACAAAAAGGGAATGAGAGTGG
PRIMER_RIGHT_3_SEQUENCE=TATGATCATCCCCATCCCTAGA
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=109,22
PRIMER_LEFT_3_TM=59.499
PRIMER_RIGHT_3_TM=60.491
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=0.9913
PRIMER_LEFT_4_PENALTY=0.500551
PRIMER_RIGHT_4_PENALTY=0.490741
PRIMER_LEFT_4_SEQUENCE=AACAAAAAGGGAATGAGAGTGG
PRIMER_RIGHT_4_SEQUENCE=TCTATGATCATCCCCATCCCTA
PRIMER_LEFT_4=10,22
PRIMER_RIGHT_4=111,22
PRIMER_LEFT_4_TM=59.499
PRIMER_RIGHT_4_TM=60.491
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=102
=
PRIMER_SEQUENCE_ID=SSR_04039 Ps_contig_mira-and-tgicl-ass_franssen_31908
SEQUENCE=AGAAGCACAAAACAAAAAGGGAATGAGAGTGGCATTGGAGAAAGAAAGAACTCAAGGGATGAGGATGGCCGTGGAGAGAAAAAGAAGCTCTAGGGATGGGGATGATCATAGAGAAACAAAAATCAGAAGGATTGAGGATGACCGTACGGGAAGAAAGGCCATAAGGAATGGAGATGATTATGGAGAAAGAAATCACTCGAGGAATGAGGGCCGCCATAGAGAAGCACCAAACAAAAGGGATGGGGCTGGCAGTGGAGAAGTAAAGAACTCAAGAGATGAGGATGATCGCAAAGAAATAAAGAACTCAAGGGGTGAGGATGATTACAAGGAGAGAAAGAGCTCCAGGGATGAGGATCTTCGTGAGGAAAGAAGAGAAGCACCAAACAGAAGGGACGGGGCTGGCAGTGGAGAAGTTAAGAACTCAAGGAACGAGGAAGATCGCAGAGAAAGAAAGAACTCAAGGGATGAGGATGATTATAAGGAGAGAAGGAGCTCTAGGGATGAGGATGTGCGTGAGGAAAGAAAGCACAGAGGGGGTGAGGATTATCATGGAGAAAGAAAGCGCAGAAGGGATGATGATGATCATGTGCATGGAGAAAGAAGGCATTATAGAAGGGATGATGATGATGGAGAAAGAAAGTACAGAAGGGAGTAGTTTGAGCAGGAAAATGAAGTTTATAAGAGGGATATCCATGTGAATTACTCCCAGAAAACCAAATTCAATGTATCTAGTTCAAGGGAGAGCATGAAAACCCAATACCGTAGTCCAAGGATTTAGAATGCTGATGTTGTGCTTTAGCCTTTGAATGTGTCTTTTAGTTCTTTTTTTAGTTGTTCTTGTCGTTTTCTGCTTAGTTTAAAAGCGTTTTAG
TARGET=445,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1546
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.152322
PRIMER_LEFT_SEQUENCE=GAGGAAAGAAGAGAAGCACCAA
PRIMER_RIGHT_SEQUENCE=TTCTCCATGATAATCCTCACCC
PRIMER_LEFT=361,22
PRIMER_RIGHT=555,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.152
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.2304
PRIMER_LEFT_1_PENALTY=0.078029
PRIMER_RIGHT_1_PENALTY=0.152322
PRIMER_LEFT_1_SEQUENCE=AAGAGAAGCACCAAACAGAAGG
PRIMER_RIGHT_1_SEQUENCE=TTCTCCATGATAATCCTCACCC
PRIMER_LEFT_1=369,22
PRIMER_RIGHT_1=555,22
PRIMER_LEFT_1_TM=59.922
PRIMER_RIGHT_1_TM=60.152
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.2826
PRIMER_LEFT_2_PENALTY=0.173354
PRIMER_RIGHT_2_PENALTY=0.109273
PRIMER_LEFT_2_SEQUENCE=CTCAAGGGGTGAGGATGATTAC
PRIMER_RIGHT_2_SEQUENCE=TCCCTAGAGCTCCTTCTCTCCT
PRIMER_LEFT_2=303,22
PRIMER_RIGHT_2=500,22
PRIMER_LEFT_2_TM=59.827
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=0.2827
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.152322
PRIMER_LEFT_3_SEQUENCE=TTCGTGAGGAAAGAAGAGAAGC
PRIMER_RIGHT_3_SEQUENCE=TTCTCCATGATAATCCTCACCC
PRIMER_LEFT_3=356,22
PRIMER_RIGHT_3=555,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=60.152
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=0.2880
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.285689
PRIMER_LEFT_4_SEQUENCE=GAGGAAAGAAGAGAAGCACCAA
PRIMER_RIGHT_4_SEQUENCE=ATCCTCATCCCTAGAGCTCCTT
PRIMER_LEFT_4=361,22
PRIMER_RIGHT_4=507,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=59.714
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_04040 Ps_contig_mira-and-tgicl-ass_franssen_31908
SEQUENCE=AGAAGCACAAAACAAAAAGGGAATGAGAGTGGCATTGGAGAAAGAAAGAACTCAAGGGATGAGGATGGCCGTGGAGAGAAAAAGAAGCTCTAGGGATGGGGATGATCATAGAGAAACAAAAATCAGAAGGATTGAGGATGACCGTACGGGAAGAAAGGCCATAAGGAATGGAGATGATTATGGAGAAAGAAATCACTCGAGGAATGAGGGCCGCCATAGAGAAGCACCAAACAAAAGGGATGGGGCTGGCAGTGGAGAAGTAAAGAACTCAAGAGATGAGGATGATCGCAAAGAAATAAAGAACTCAAGGGGTGAGGATGATTACAAGGAGAGAAAGAGCTCCAGGGATGAGGATCTTCGTGAGGAAAGAAGAGAAGCACCAAACAGAAGGGACGGGGCTGGCAGTGGAGAAGTTAAGAACTCAAGGAACGAGGAAGATCGCAGAGAAAGAAAGAACTCAAGGGATGAGGATGATTATAAGGAGAGAAGGAGCTCTAGGGATGAGGATGTGCGTGAGGAAAGAAAGCACAGAGGGGGTGAGGATTATCATGGAGAAAGAAAGCGCAGAAGGGATGATGATGATCATGTGCATGGAGAAAGAAGGCATTATAGAAGGGATGATGATGATGGAGAAAGAAAGTACAGAAGGGAGTAGTTTGAGCAGGAAAATGAAGTTTATAAGAGGGATATCCATGTGAATTACTCCCAGAAAACCAAATTCAATGTATCTAGTTCAAGGGAGAGCATGAAAACCCAATACCGTAGTCCAAGGATTTAGAATGCTGATGTTGTGCTTTAGCCTTTGAATGTGTCTTTTAGTTCTTTTTTTAGTTGTTCTTGTCGTTTTCTGCTTAGTTTAAAAGCGTTTTAG
TARGET=572,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2100
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=GAGGAAGATCGCAGAGAAAGAA
PRIMER_RIGHT_SEQUENCE=TCCTGCTCAAACTACTCCCTTC
PRIMER_LEFT=430,22
PRIMER_RIGHT=665,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.2182
PRIMER_LEFT_1_PENALTY=0.109273
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=AGGAGAGAAGGAGCTCTAGGGA
PRIMER_RIGHT_1_SEQUENCE=GGACTACGGTATTGGGTTTTCA
PRIMER_LEFT_1=479,22
PRIMER_RIGHT_1=767,22
PRIMER_LEFT_1_TM=60.109
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=289
PRIMER_PAIR_PENALTY_2=0.2182
PRIMER_LEFT_2_PENALTY=0.109273
PRIMER_RIGHT_2_PENALTY=0.108929
PRIMER_LEFT_2_SEQUENCE=AGGAGAGAAGGAGCTCTAGGGA
PRIMER_RIGHT_2_SEQUENCE=TGGACTACGGTATTGGGTTTTC
PRIMER_LEFT_2=479,22
PRIMER_RIGHT_2=768,22
PRIMER_LEFT_2_TM=60.109
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.2237
PRIMER_LEFT_3_PENALTY=0.109273
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=AGGAGAGAAGGAGCTCTAGGGA
PRIMER_RIGHT_3_SEQUENCE=TCCTGCTCAAACTACTCCCTTC
PRIMER_LEFT_3=479,22
PRIMER_RIGHT_3=665,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.2680
PRIMER_LEFT_4_PENALTY=0.109273
PRIMER_RIGHT_4_PENALTY=0.158697
PRIMER_LEFT_4_SEQUENCE=AGGAGAGAAGGAGCTCTAGGGA
PRIMER_RIGHT_4_SEQUENCE=GGTATTGGGTTTTCATGCTCTC
PRIMER_LEFT_4=479,22
PRIMER_RIGHT_4=760,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=59.841
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_04041 Ps_contig_mira-and-tgicl-ass_franssen_31908
SEQUENCE=AGAAGCACAAAACAAAAAGGGAATGAGAGTGGCATTGGAGAAAGAAAGAACTCAAGGGATGAGGATGGCCGTGGAGAGAAAAAGAAGCTCTAGGGATGGGGATGATCATAGAGAAACAAAAATCAGAAGGATTGAGGATGACCGTACGGGAAGAAAGGCCATAAGGAATGGAGATGATTATGGAGAAAGAAATCACTCGAGGAATGAGGGCCGCCATAGAGAAGCACCAAACAAAAGGGATGGGGCTGGCAGTGGAGAAGTAAAGAACTCAAGAGATGAGGATGATCGCAAAGAAATAAAGAACTCAAGGGGTGAGGATGATTACAAGGAGAGAAAGAGCTCCAGGGATGAGGATCTTCGTGAGGAAAGAAGAGAAGCACCAAACAGAAGGGACGGGGCTGGCAGTGGAGAAGTTAAGAACTCAAGGAACGAGGAAGATCGCAGAGAAAGAAAGAACTCAAGGGATGAGGATGATTATAAGGAGAGAAGGAGCTCTAGGGATGAGGATGTGCGTGAGGAAAGAAAGCACAGAGGGGGTGAGGATTATCATGGAGAAAGAAAGCGCAGAAGGGATGATGATGATCATGTGCATGGAGAAAGAAGGCATTATAGAAGGGATGATGATGATGGAGAAAGAAAGTACAGAAGGGAGTAGTTTGAGCAGGAAAATGAAGTTTATAAGAGGGATATCCATGTGAATTACTCCCAGAAAACCAAATTCAATGTATCTAGTTCAAGGGAGAGCATGAAAACCCAATACCGTAGTCCAAGGATTTAGAATGCTGATGTTGTGCTTTAGCCTTTGAATGTGTCTTTTAGTTCTTTTTTTAGTTGTTCTTGTCGTTTTCTGCTTAGTTTAAAAGCGTTTTAG
TARGET=617,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1469
PRIMER_LEFT_PENALTY=0.037936
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=ATGATCATGTGCATGGAGAAAG
PRIMER_RIGHT_SEQUENCE=GGACTACGGTATTGGGTTTTCA
PRIMER_LEFT=578,22
PRIMER_RIGHT=767,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=0.1469
PRIMER_LEFT_1_PENALTY=0.037936
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=ATGATCATGTGCATGGAGAAAG
PRIMER_RIGHT_1_SEQUENCE=TGGACTACGGTATTGGGTTTTC
PRIMER_LEFT_1=578,22
PRIMER_RIGHT_1=768,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.1899
PRIMER_LEFT_2_PENALTY=0.080962
PRIMER_RIGHT_2_PENALTY=0.108929
PRIMER_LEFT_2_SEQUENCE=GATCATGTGCATGGAGAAAGAA
PRIMER_RIGHT_2_SEQUENCE=GGACTACGGTATTGGGTTTTCA
PRIMER_LEFT_2=580,22
PRIMER_RIGHT_2=767,22
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.1899
PRIMER_LEFT_3_PENALTY=0.080962
PRIMER_RIGHT_3_PENALTY=0.108929
PRIMER_LEFT_3_SEQUENCE=GATCATGTGCATGGAGAAAGAA
PRIMER_RIGHT_3_SEQUENCE=TGGACTACGGTATTGGGTTTTC
PRIMER_LEFT_3=580,22
PRIMER_RIGHT_3=768,22
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=60.109
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.1966
PRIMER_LEFT_4_PENALTY=0.037936
PRIMER_RIGHT_4_PENALTY=0.158697
PRIMER_LEFT_4_SEQUENCE=ATGATCATGTGCATGGAGAAAG
PRIMER_RIGHT_4_SEQUENCE=GTATTGGGTTTTCATGCTCTCC
PRIMER_LEFT_4=578,22
PRIMER_RIGHT_4=759,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=59.841
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_04042 Ps_contig_mira-and-tgicl-ass_franssen_31909
SEQUENCE=GTCGGGGGAAGCACAGTGAAATTTTCACATCAATGGAAGAACAACAAAAGCTTCTTCAATCCTCTTCCTACCCCTTCTTCTGGGGCTACACACCAGAACAAGAATACTACAAACAACAACAAATCACATCCTCAAAATCAACCTTCACAACACCAAGAAATCTCACCCTCTTCACCCGATCATGGCTTCCAAACCTCACCACCACCCCACCACGCGCTCTCATCTTCATGATCCATGGCTACGGCAACGACATCTCATGGACCTTTCAATCCACCCCAATCTTCCTTGCTCAAATGGGTTTCGCTTGTTTCGCTCTGGACCTTCAAGGTCACGGTCAAACCCAGGGTCTTAAAGCTTTTGTCCCTAGCGTTGACCTTGTTGTACAAGATTGTCTCTCTTACTTCAATTCCATCAAAAGAGATCCAAAGTTTAAAGCTTTACCTTGTTTTCTATTCGGTGAATCAATGGGTGGTGCTATTTGTCTTTTGATTCATTTTGCTGATCCAAGAGGCTTTCAAGGTGCTATCTTGGTAGCACCCATGTGTAAAATCTCTGATAAGGTTAGACCCAAATGGCCAATTCCTCAGATTCTCACGTTTCTTGCAAGATTCTTCCCTACTTTGCCGATTGTTCCTACTCCTGATCTTCTGTACAAGTCTGTGAAAGTTGATCATAAGAAGGTTATTGCTGAAATGAATCCTTTGAGGTATAGAGGGAAACCTAGGTTAGGGACTGTGGTGGAGCTTTTGAGGGTTACTGATATTTTGAGTAGGAGGCTTTGTGATGTTGAGCTTCCTTTTATTGTGTTACATGGTAGTGCTGATGTTGTTACTGATCCTGAGGTTAGTAGGGAGTTGTATGAAGAAGCTAGGAGTGATGATAAGACTATTGAGGTGTATGATGGGATGATGCATTCTTTGCTTTTTGGGGAGACTGATGAGAATGTTAAAATTGTTAGGAGTGATATTTTGCAGTGGTTGAATGCTAGATGCAATTGAAGAACAAGTGGTGATTGTAGATGAACATGATTGTTGTGATGATTGATGAAGTGGAAGGCTTGAAAGGATGTTTGCAATTGTAAGTAGTTTGAGTTCTCAATTGTTCTGTTATTGATTCTAGATTGATGAAATGCATCAATGAGGATTTGAATTAAATTACTGGTCTATAGTATTAGTATACATAGTTGTTTTATTTATTTATAAAAGATATGATTCTTGGAATGACATGTAGGTGTTTGTTTTGGTGTG
TARGET=1188,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5230
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=1.491216
PRIMER_LEFT_SEQUENCE=ATGATTGATGAAGTGGAAGGCT
PRIMER_RIGHT_SEQUENCE=CACACCAAAACAAACACCTACA
PRIMER_LEFT=1036,22
PRIMER_RIGHT=1246,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=58.509
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=1.5587
PRIMER_LEFT_1_PENALTY=0.067436
PRIMER_RIGHT_1_PENALTY=1.491216
PRIMER_LEFT_1_SEQUENCE=TGATGATTGATGAAGTGGAAGG
PRIMER_RIGHT_1_SEQUENCE=CACACCAAAACAAACACCTACA
PRIMER_LEFT_1=1034,22
PRIMER_RIGHT_1=1246,22
PRIMER_LEFT_1_TM=59.933
PRIMER_RIGHT_1_TM=58.509
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=1.6264
PRIMER_LEFT_2_PENALTY=0.031769
PRIMER_RIGHT_2_PENALTY=1.594644
PRIMER_LEFT_2_SEQUENCE=ATGATTGATGAAGTGGAAGGCT
PRIMER_RIGHT_2_SEQUENCE=CAAACACCTACATGTCATTCCA
PRIMER_LEFT_2=1036,22
PRIMER_RIGHT_2=1236,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=58.405
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=1.6452
PRIMER_LEFT_3_PENALTY=0.154023
PRIMER_RIGHT_3_PENALTY=1.491216
PRIMER_LEFT_3_SEQUENCE=GCAATTGAAGAACAAGTGGTGA
PRIMER_RIGHT_3_SEQUENCE=CACACCAAAACAAACACCTACA
PRIMER_LEFT_3=990,22
PRIMER_RIGHT_3=1246,22
PRIMER_LEFT_3_TM=60.154
PRIMER_RIGHT_3_TM=58.509
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=1.8766
PRIMER_LEFT_4_PENALTY=0.385416
PRIMER_RIGHT_4_PENALTY=1.491216
PRIMER_LEFT_4_SEQUENCE=GATGCAATTGAAGAACAAGTGG
PRIMER_RIGHT_4_SEQUENCE=CACACCAAAACAAACACCTACA
PRIMER_LEFT_4=987,22
PRIMER_RIGHT_4=1246,22
PRIMER_LEFT_4_TM=59.615
PRIMER_RIGHT_4_TM=58.509
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=260
=
PRIMER_SEQUENCE_ID=SSR_04043 Ps_contig_mira-and-tgicl-ass_franssen_31912
SEQUENCE=TCGGGGATGATACTATCACATAACGCAGTTACGAAAATAGCCCCAAATCAGTTTCTCCAAGTCCCCTCCGCGAAACGCAGGAATCGCGTAAGGCAGCAGTGAGCAATCATCAGTCATCACCGACTACCGAGATTTGCACAACGCCAAAGCTAAACAAATCTGAGTGAGCTTTAAGGATGGCTCCGGTTGCTCCTCGATCTGGCGATGCGATATTTGCTAACATCGAACGCGTTAATGCGGAGCTGTTTACTCTGACATATGGGGCAATTGTGCGGCAATTGCTTACAGATCTGGAAGAGGTTGAGGAAGTCAACAAACAGCTAGATCAAATGGGTTATAATATTGGAATCCGTTTGATTGATGAGTTTTTGGCTAAATCTAATGTCTCAAGGTGTGTTGATTTCAGAGAGACCGCTGATGTGATTGCAAAGGTTGGTTTTAAAATGTTTCTCGGTGTTACTGCATCCGTGACCAATTGGGATGCTGAAGGAACTTGTTGTAGTATTGTTTTGGAAGATAATCCTTTGGTGGACTTTGTTGAGCTTCCTGACAACTACCAAGGTCTGTACTACTGCAACATATTAAGCGGAGTTATTCGAGGAGCCTTGGATATGGTATCAATGAAGGCTGAGGTCACTTGGCTACGTGATGCGCTTCGAGGTGATGATGTGTTTGAGTTGCAAGTAAAACTTCTCAAGCAAGTTCCAGAAGAGTATCCATATAAGGATGATGAGTAGTAATGTTCTGTTTTGTATTGTTGTACTTCATCATCCCAAGTATTAGATTATTATTATTATCTTTCTGCCTTGTGTGCATTTTGACAAAACTAAAAATACGATAGAAAATATTTAATTAAATCATTATGATAATTGGGATTTTATAAGAATTCYTCAATTGTTC
TARGET=784,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04044 Ps_contig_mira-and-tgicl-ass_franssen_31916
SEQUENCE=AGACCATAGACAATTAGACATCATCCCCAGTTCAATTCTTCTTCGAATTATAAACGAATACGATGTCGTTTGGATCTTCTCTGTTATCTCCTCCGCCGCATCTTGTTTGCCGGAAACGTAACAGAATCTCTCCGTTCAGGGTCGCTGCAAACGTTCCCGATTTCCTCTCTGCAGACTGGTTTGAATCTCGCAAGAAAAAACCGTTTGGTCCAAGATTAGATTTCTCTGCAGAAGATGCCGTTCATCATCAGCTTGAAGCGTTGAAGTACAACGATCAGCCTCGTCAAGATTATGGAATTGAAGTCATGTACAGGTTTGCTGGATTTGATCCTTTTGAAAGATCAACCTATTTTGGACCATTCTTTGATCTCGGCCAGTTTGAAAGGTTCAGGCGTATTTTTCATCATTCTACTTACAGAGTTTTATTGGGTCACAAAGAAAGAAAGATCATGAGTAGTTTATTTGTGGAGGAGAACAAGTATAAACAACGGATTTGGGTTCGTGGATGTCGACCTGAGGAAGAGGAGATATTTCAATTTACAATGGTTCAGAGGGTTGGTGGTTGCTGGGATGGTTATTGGCTGACTGAGTCACTGCTTCACAATACAGATACATTTTCTGGTGGGTTAGCTTATTGAAAATCATTCAATGGAGAAGAAATCAGTTTCATATTTGAAAAACATAGTGATATACTATTACTATCCTAATCATAGTATGTAAAACTTGTACATATTTGTAAGAAATTCAGCTGTAATTATGTGTAAATAAACAAGGACTATAATTTGTCCTCGATGATCCRTCTCTAATTANCGG
TARGET=435,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04045 Ps_contig_mira-and-tgicl-ass_franssen_31937
SEQUENCE=TCGGGGTAANTCCGTAATTTCAGCGTAACGTTTACTAACGCCATCATGTCCACTCTACTTCAATCCTCTTTCATTTCTTCTTCTTCCATCTCTCCGATCACTCCGCGTTTCGCTCGCATCTCCATCCGTAACGCCGCCAGATGTAATAATGCCGTAGTCTCTTTGAACTCGGCGAACGGAATTCCATATCGTTCCACTGAACTTTCAGTTACCGGTTCTACTTCCATTCCGAGTTTTCTTGTGGCGCCGAACACTCCCATCCATGATCTCGTTAACAGAAATCGTCTTCGTATATTCTCCGGTACCGCCAATCCCGCACTCGCTCAGGAAATTGCATGCTATATGGGCCTGGATCTGGGAAAGATTAAGATAAAACGTTTTGCAGATGGCGAGATATATGTCCAACTACAAGAGAGTGTCAGGGGGTGTGAATAAAGTAAAGTTTAACTCGTGCAGTCCACATGTCCTCCT
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1243
PRIMER_LEFT_PENALTY=0.110942
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=TTACTAACGCCATCATGTCCAC
PRIMER_RIGHT_SEQUENCE=ACCGGAGAATATACGAAGACGA
PRIMER_LEFT=31,22
PRIMER_RIGHT=302,22
PRIMER_LEFT_TM=59.889
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.2326
PRIMER_LEFT_1_PENALTY=0.110942
PRIMER_RIGHT_1_PENALTY=0.121635
PRIMER_LEFT_1_SEQUENCE=TTACTAACGCCATCATGTCCAC
PRIMER_RIGHT_1_SEQUENCE=CGGTACCGGAGAATATACGAAG
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=306,22
PRIMER_LEFT_1_TM=59.889
PRIMER_RIGHT_1_TM=59.878
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.3897
PRIMER_LEFT_2_PENALTY=0.376347
PRIMER_RIGHT_2_PENALTY=0.013401
PRIMER_LEFT_2_SEQUENCE=TTTACTAACGCCATCATGTCCA
PRIMER_RIGHT_2_SEQUENCE=ACCGGAGAATATACGAAGACGA
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=302,22
PRIMER_LEFT_2_TM=60.376
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.4434
PRIMER_LEFT_3_PENALTY=0.429952
PRIMER_RIGHT_3_PENALTY=0.013401
PRIMER_LEFT_3_SEQUENCE=GCGTAACGTTTACTAACGCCAT
PRIMER_RIGHT_3_SEQUENCE=ACCGGAGAATATACGAAGACGA
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=302,22
PRIMER_LEFT_3_TM=60.430
PRIMER_RIGHT_3_TM=59.987
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.4796
PRIMER_LEFT_4_PENALTY=0.110942
PRIMER_RIGHT_4_PENALTY=0.368624
PRIMER_LEFT_4_SEQUENCE=TTACTAACGCCATCATGTCCAC
PRIMER_RIGHT_4_SEQUENCE=ACAAGAAAACTCGGAATGGAAG
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=241,22
PRIMER_LEFT_4_TM=59.889
PRIMER_RIGHT_4_TM=59.631
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_04046 Ps_contig_mira-and-tgicl-ass_franssen_31945
SEQUENCE=AGTANTCGGNTGACAACACAATTCTCCTTCTCTTCTCTTCTCTTATTATCCCTCTTCTTTCTTTCTTCTCAATTCCATCCGTTATATTCACTTTCCATCTCAATTCCAATGAAGTTCATTAACCCTAAAAACCTCTCTCCCAAACACCTTTTCCGTTCCAAGAAGGAAAAATCATCCGTATCACGATCCGACCCGCTTTCCTTCGGGTCAGCTTCTTCCTCTGACGACTCCACTCACAAAGCCGTCACCGGAGGCTCTCAAACACCTACCAGCGTCCTTCCTGAAGCATCGTCCAGCTCGTGGTCCGACGTCACCGTCGAAGTTCAATGGGAGCTTGCTCAGGCGTTCAGATTAATCGACCGCGACAACGACGGCGTCGTTTCGCGTGAAGAACTCGAAGCCGTTCTGACGCGCCTCGGAGCGCGTCCGCCTAGCTCCGAGGAGATCGAGATGATGCTAAGCGAAGTTGATAGCGATGGTAGAGGATGCATAAGCGTGGAATCGATCATGAACCGGATTGGATCGGGTTCGGCTTCGGCTTCGGATCCGAATCCGGAGGAAGAACTGAGGGAAGCGTTTGAGGTTTTTGATACGGATCGGGATGGGAGAATCTCCGCGGAAGAGTTGTTGAGGGTTTTTAGAGCAATTGGGGATGAACGGTGTACGTTAGAGGAGTGCCGGCGTATGATAGCAGGTGTTGATAAAAACAGAGATGGGTTTGTCTGTTTCGAGGAGTTTTCGTGTATGATGGAGATGCAGCGATGATGGTGACTGTGATTATGAAAGAAAAAAATAACAATATAATAATATAAATATATTACTAAATGAAAGAAAAAAAACAATTTCTTTGANCATACTAATTACTTAATTAGTTTAGTTTAACTTTAACTTTAAGTTACCGTTTTCTTT
TARGET=27,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2985
PRIMER_LEFT_PENALTY=1.251261
PRIMER_RIGHT_PENALTY=0.047267
PRIMER_LEFT_SEQUENCE=TCGGNTGACAACACAATTCTC
PRIMER_RIGHT_SEQUENCE=GGATCGTGATACGGATGATTTT
PRIMER_LEFT=5,21
PRIMER_RIGHT=188,22
PRIMER_LEFT_TM=59.749
PRIMER_RIGHT_TM=60.047
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.50
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=1.4552
PRIMER_LEFT_1_PENALTY=1.251261
PRIMER_RIGHT_1_PENALTY=0.203971
PRIMER_LEFT_1_SEQUENCE=TCGGNTGACAACACAATTCTC
PRIMER_RIGHT_1_SEQUENCE=CGTGATACGGATGATTTTTCCT
PRIMER_LEFT_1=5,21
PRIMER_RIGHT_1=184,22
PRIMER_LEFT_1_TM=59.749
PRIMER_RIGHT_1_TM=60.204
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.50
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=1.5845
PRIMER_LEFT_2_PENALTY=1.251261
PRIMER_RIGHT_2_PENALTY=0.333282
PRIMER_LEFT_2_SEQUENCE=TCGGNTGACAACACAATTCTC
PRIMER_RIGHT_2_SEQUENCE=TGGGAGAGAGGTTTTTAGGGTT
PRIMER_LEFT_2=5,21
PRIMER_RIGHT_2=141,22
PRIMER_LEFT_2_TM=59.749
PRIMER_RIGHT_2_TM=60.333
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.50
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=1.5845
PRIMER_LEFT_3_PENALTY=1.251261
PRIMER_RIGHT_3_PENALTY=0.333282
PRIMER_LEFT_3_SEQUENCE=TCGGNTGACAACACAATTCTC
PRIMER_RIGHT_3_SEQUENCE=TTGGGAGAGAGGTTTTTAGGGT
PRIMER_LEFT_3=5,21
PRIMER_RIGHT_3=142,22
PRIMER_LEFT_3_TM=59.749
PRIMER_RIGHT_3_TM=60.333
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.50
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=1.6321
PRIMER_LEFT_4_PENALTY=1.251261
PRIMER_RIGHT_4_PENALTY=0.380820
PRIMER_LEFT_4_SEQUENCE=TCGGNTGACAACACAATTCTC
PRIMER_RIGHT_4_SEQUENCE=AAGGTGTTTGGGAGAGAGGTTT
PRIMER_LEFT_4=5,21
PRIMER_RIGHT_4=149,22
PRIMER_LEFT_4_TM=59.749
PRIMER_RIGHT_4_TM=60.381
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.50
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_04047 Ps_contig_mira-and-tgicl-ass_franssen_31945
SEQUENCE=AGTANTCGGNTGACAACACAATTCTCCTTCTCTTCTCTTCTCTTATTATCCCTCTTCTTTCTTTCTTCTCAATTCCATCCGTTATATTCACTTTCCATCTCAATTCCAATGAAGTTCATTAACCCTAAAAACCTCTCTCCCAAACACCTTTTCCGTTCCAAGAAGGAAAAATCATCCGTATCACGATCCGACCCGCTTTCCTTCGGGTCAGCTTCTTCCTCTGACGACTCCACTCACAAAGCCGTCACCGGAGGCTCTCAAACACCTACCAGCGTCCTTCCTGAAGCATCGTCCAGCTCGTGGTCCGACGTCACCGTCGAAGTTCAATGGGAGCTTGCTCAGGCGTTCAGATTAATCGACCGCGACAACGACGGCGTCGTTTCGCGTGAAGAACTCGAAGCCGTTCTGACGCGCCTCGGAGCGCGTCCGCCTAGCTCCGAGGAGATCGAGATGATGCTAAGCGAAGTTGATAGCGATGGTAGAGGATGCATAAGCGTGGAATCGATCATGAACCGGATTGGATCGGGTTCGGCTTCGGCTTCGGATCCGAATCCGGAGGAAGAACTGAGGGAAGCGTTTGAGGTTTTTGATACGGATCGGGATGGGAGAATCTCCGCGGAAGAGTTGTTGAGGGTTTTTAGAGCAATTGGGGATGAACGGTGTACGTTAGAGGAGTGCCGGCGTATGATAGCAGGTGTTGATAAAAACAGAGATGGGTTTGTCTGTTTCGAGGAGTTTTCGTGTATGATGGAGATGCAGCGATGATGGTGACTGTGATTATGAAAGAAAAAAATAACAATATAATAATATAAATATATTACTAAATGAAAGAAAAAAAACAATTTCTTTGANCATACTAATTACTTAATTAGTTTAGTTTAACTTTAACTTTAAGTTACCGTTTTCTTT
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0584
PRIMER_LEFT_PENALTY=0.854451
PRIMER_RIGHT_PENALTY=0.203971
PRIMER_LEFT_SEQUENCE=GGNTGACAACACAATTCTCCTT
PRIMER_RIGHT_SEQUENCE=CGTGATACGGATGATTTTTCCT
PRIMER_LEFT=7,22
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=59.146
PRIMER_RIGHT_TM=60.204
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.50
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=1.1877
PRIMER_LEFT_1_PENALTY=0.854451
PRIMER_RIGHT_1_PENALTY=0.333282
PRIMER_LEFT_1_SEQUENCE=GGNTGACAACACAATTCTCCTT
PRIMER_RIGHT_1_SEQUENCE=TGGGAGAGAGGTTTTTAGGGTT
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=141,22
PRIMER_LEFT_1_TM=59.146
PRIMER_RIGHT_1_TM=60.333
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.50
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=1.1877
PRIMER_LEFT_2_PENALTY=0.854451
PRIMER_RIGHT_2_PENALTY=0.333282
PRIMER_LEFT_2_SEQUENCE=GGNTGACAACACAATTCTCCTT
PRIMER_RIGHT_2_SEQUENCE=TTGGGAGAGAGGTTTTTAGGGT
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=142,22
PRIMER_LEFT_2_TM=59.146
PRIMER_RIGHT_2_TM=60.333
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.50
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=1.2353
PRIMER_LEFT_3_PENALTY=0.854451
PRIMER_RIGHT_3_PENALTY=0.380820
PRIMER_LEFT_3_SEQUENCE=GGNTGACAACACAATTCTCCTT
PRIMER_RIGHT_3_SEQUENCE=AAAGGTGTTTGGGAGAGAGGTT
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=150,22
PRIMER_LEFT_3_TM=59.146
PRIMER_RIGHT_3_TM=60.381
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.50
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=1.2353
PRIMER_LEFT_4_PENALTY=0.854451
PRIMER_RIGHT_4_PENALTY=0.380820
PRIMER_LEFT_4_SEQUENCE=GGNTGACAACACAATTCTCCTT
PRIMER_RIGHT_4_SEQUENCE=AAGGTGTTTGGGAGAGAGGTTT
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=149,22
PRIMER_LEFT_4_TM=59.146
PRIMER_RIGHT_4_TM=60.381
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.50
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_04048 Ps_contig_mira-and-tgicl-ass_franssen_31947
SEQUENCE=AGTACGCGNGATCGAACAAAACGAGCCCATCGAACCCTCCAACGCTTTGGTTTCTTCATCACCACCACCTTTTCATCTCTCTCTCTCTAAAAGCCATAAACAACAAACAAGAAATAATAATCGAAAACGCAAAAAGGGCTTTTCCGAGTTAGATCTAGGGTTTCCATCGATTCTAAAACTGGATATTGATCGAGCAACAAAGGGTTAGGAGGTGGAGGATTTCGAAATGGATTCGGTGCCTTCGAATTCCCATGGAAATCTCGATGAACAGATTTCTCAGCTCATGCAGTGCAAACCCTTGTCTGAACAAGAGGTTAGGGTTTTGTGTGATAAGGCGAAGGAGATTTTGATGGAAGAGAGCAATGTCCAGCCTGTAAAAAGTCCTGTTACAATCTGTGGTGATATTCATGGGCAGTTCCATGATCTTGCAGAGCTTTTCCGCATTGGAGGGAAGTGTCCAGATACAAATTACTTGTTTATGGGAGATTATGTTGATCGTGGCTATTATTCTGTCGAAACCGTAACGCTTTTGGTCTCTCTTAAAGTGCGTTATCGTCAGCGTATTACTATTTTGAGGGGAAATCATGAAAGTCGTCAGATTACTCAAGTTTATGGATTTTATGACGAGTGCCTACGGAAGTATGGTAGTGCTAATGTTTGGAAGATGTTCACTGATTTGTTTGACTACTTCCCGTTGACAGCATTGGTTGAATCTGAAATATTTTGTCTTCATGGTGGGTTGTCCCCGTCTATTGAAACCCTTGACAACATTCGTAATTTTGATCGCGTGCAAGAAGTTCCCCATGAGGGACCCATGTGTGATCTTTTATGGTCTGATCCGGATGATCGCTGTGGTTGGGGTATCTCTCCTAGAGGTGCTGGATACACTTTTGGCCAGGACATATCTGAGCAGTTTAACCATACCAATAATTTGAAGCTGATTGCTAGAGCTCACCAGCTGGTTATGGAAGGATATAACTGGGGTCATGATCAAAAGGTGGTTACCATATTCAGTGCACCTAATTACTGTTATCGCTGTGGGAACATGGCATCCATCCTTGAAGTTGATGACTGCAAAGGACACACATTCATTCAGTTTGAGCCAGCTCCAAGGAGGGGAGAGCCAGATGTAACTAGGAGAACCCCTGACTACTTCCTATGATACAATTATGGTAGAGTTGCTGTTGGCAGCAACTTGTGCCCTGCAGTAATATGCAAACTTCATATACCAGTTGAAATGAGAGCTGTCAATCAAACTGATCCTTATTTTCAAAGTTAGGCTTCACTGGACGTGTTTTTTTTACTTGAATTATAATTGAACCTTTCCAAGAGAGAAGCTGAGGAACAGGAGATGCTATTGTGGTCATGTGTACCGTTCCAGCATGCAATGTATCAATTTGAATGTGTCTCAAATTTTCCTTTTCTATTTATATGCAACTCTGTTGACCTTTCTTTTTTTCATTTTAGGGTTATTGTTAATGGGGATGAGTTTCTGTAGATGGACAATGCTTTGACTATCTTTTAATGGGTGTTTTTATGATTTTTTTTCAACCAGTCTTGTGTAGTTTTGTTTTCTTCGTTTTACTTGAAATGTAATTTTATTTTTTTAA
TARGET=76,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2902
PRIMER_LEFT_PENALTY=0.267873
PRIMER_RIGHT_PENALTY=0.022302
PRIMER_LEFT_SEQUENCE=GTTTCTTCATCACCACCACCTT
PRIMER_RIGHT_SEQUENCE=TGCATGAGCTGAGAAATCTGTT
PRIMER_LEFT=49,22
PRIMER_RIGHT=287,22
PRIMER_LEFT_TM=60.268
PRIMER_RIGHT_TM=60.022
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.3633
PRIMER_LEFT_1_PENALTY=0.267873
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=GTTTCTTCATCACCACCACCTT
PRIMER_RIGHT_1_SEQUENCE=AAATCTCCTTCGCCTTATCACA
PRIMER_LEFT_1=49,22
PRIMER_RIGHT_1=346,22
PRIMER_LEFT_1_TM=60.268
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.5060
PRIMER_LEFT_2_PENALTY=0.267873
PRIMER_RIGHT_2_PENALTY=0.238160
PRIMER_LEFT_2_SEQUENCE=GTTTCTTCATCACCACCACCTT
PRIMER_RIGHT_2_SEQUENCE=AGATCTAACTCGGAAAAGCCCT
PRIMER_LEFT_2=49,22
PRIMER_RIGHT_2=155,22
PRIMER_LEFT_2_TM=60.268
PRIMER_RIGHT_2_TM=59.762
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=107
PRIMER_PAIR_PENALTY_3=0.5445
PRIMER_LEFT_3_PENALTY=0.522246
PRIMER_RIGHT_3_PENALTY=0.022302
PRIMER_LEFT_3_SEQUENCE=AACGCTTTGGTTTCTTCATCAC
PRIMER_RIGHT_3_SEQUENCE=TGCATGAGCTGAGAAATCTGTT
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=287,22
PRIMER_LEFT_3_TM=60.522
PRIMER_RIGHT_3_TM=60.022
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=248
PRIMER_PAIR_PENALTY_4=0.6131
PRIMER_LEFT_4_PENALTY=0.267873
PRIMER_RIGHT_4_PENALTY=0.345221
PRIMER_LEFT_4_SEQUENCE=GTTTCTTCATCACCACCACCTT
PRIMER_RIGHT_4_SEQUENCE=CCTCTTGTTCAGACAAGGGTTT
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=313,22
PRIMER_LEFT_4_TM=60.268
PRIMER_RIGHT_4_TM=59.655
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_04049 Ps_contig_mira-and-tgicl-ass_franssen_31954
SEQUENCE=AAGAATATAACAATCAACAAACATCATCTTCTCCGAGTATAGATCTGCAGCATCACCACCGTGAACATGGCGTTTCTCCGCTTTCTTCCACAACCACTCAATTCATCATTATCTACTGATTTCCTTCATCATCATCACCGTCTTCCCTATCCTCAACAACAACAACAACATCGCCTCAATTTCGCCGTCGTAAAAACCACTGCTCTCTCAGATTCTCATCCGTCTAGAACCTTACTTGATTCGAAAACTCAAGTGTTTGATGCTGTTCCTCTTTTATCTTCCTCCCAGGCCTATGAAAGGATGAAAGCATTCAGAGAAAATATAAAAGGAAAGCAGCAATATCTTGCCATGTATTCTAGTATTTTTGGTGGGATAACAACTGATCCAGCAGTTATGATTATTCCTATGGATGACCACATGGTCCATAGGGGACATGGTGTCTTTGATACTGCAGCAATAATGGACGGGTATCTGTATGAGTTAGATCAACATCTTGATCGCTTTTTAAATTCAGCATCAAGATCGAAAATAGATCTCCCGTTTGATCGTGAAAGTATTAGAAGAATTCTTATACAAACTGTGAGCGCTTCCAAATGTCGAAATGGATCACTAAGATATTGGTTATCAGCAGGACCTGGTGATTTTAACCTCTCTCCCTCTGGCTGCCACCAGTCAGTGCTTTATGCAATTGTAATACAGGACATGTCACCGGCAGCGGGCGCAGTTAAGTCCAGGGGAGTTAAAGTCATTACTTCATCTATTCCAATAAAACATCCCAAGTTTGCAATCACTAAGAGTGTGAATTACCTTCCAAATGTGCTTTCAAAGATGGAGGCAGAAGAAGCTGGTGCTTTTGCAGGGATTTGGCTTGACGATGAAGGTTTTGTTGCTGAAGGGCCTAATATGAATGTGGCTTTTGTCACTAAAGAGAAGGAACTTATAATGCCTCACTTTGACAAAATTCTAAGTGGGTGTACAGCTAAGAGAGTTTTAACCCTCGCTGAGCGTTTGATCGAGAAGGGTAAGCTTCGGGGGATAAGAAAGAAAAATGTGACCGTCGAAGAAGGGAAGAAAGCAGATGAAATGATGCTTCTTGGTAGCGGAGTTCTAGTTTGCCCTGTAGTGCAGTGGGATGAGAAGATTTTTGGAGATGGCAAGCCAGGTGATATAACAGAGGATCTCTTAAATCTAATTGTTGAAGACATGAAATCATCTCCCTCTATTCGTACATCTGTTCTATATTAACCCAACTTTATTTCCTTCTTAAGATGTGATTATGAACATTCATAAGTAGTTGTATCCTCAAAATTACCTACACTTTTGTTTCCCTGAACTTTCCATTTTGGCTCATTTGCACATTTGGAGATCGTGAAGTCCATTTGATCTGAATTTTGTAGCTAGGTTTGTATAACAATGTATGATGGTTGTATACCAAATTTGAAGTTCAAATTTTTTCCTTCACAATTAGGACTATTATTATGATATTACTAAAGTATTATATTTTGATTATAATATCTAATTTATATGATACG
TARGET=126,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1243
PRIMER_LEFT_PENALTY=0.110392
PRIMER_RIGHT_PENALTY=0.013872
PRIMER_LEFT_SEQUENCE=TCAACAAACATCATCTTCTCCG
PRIMER_RIGHT_SEQUENCE=ACGGATGAGAATCTGAGAGAGC
PRIMER_LEFT=13,22
PRIMER_RIGHT=222,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.986
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.2735
PRIMER_LEFT_1_PENALTY=0.163658
PRIMER_RIGHT_1_PENALTY=0.109882
PRIMER_LEFT_1_SEQUENCE=TTCCACAACCACTCAATTCATC
PRIMER_RIGHT_1_SEQUENCE=GCAAGATATTGCTGCTTTCCTT
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=346,22
PRIMER_LEFT_1_TM=59.836
PRIMER_RIGHT_1_TM=59.890
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.3303
PRIMER_LEFT_2_PENALTY=0.110392
PRIMER_RIGHT_2_PENALTY=0.219884
PRIMER_LEFT_2_SEQUENCE=TCAACAAACATCATCTTCTCCG
PRIMER_RIGHT_2_SEQUENCE=GAGGAACAGCATCAAACACTTG
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.780
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.3303
PRIMER_LEFT_3_PENALTY=0.110392
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=CAACAAACATCATCTTCTCCGA
PRIMER_RIGHT_3_SEQUENCE=GAGGAACAGCATCAAACACTTG
PRIMER_LEFT_3=14,22
PRIMER_RIGHT_3=270,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.3303
PRIMER_LEFT_4_PENALTY=0.110392
PRIMER_RIGHT_4_PENALTY=0.219884
PRIMER_LEFT_4_SEQUENCE=TCAACAAACATCATCTTCTCCG
PRIMER_RIGHT_4_SEQUENCE=GGAACAGCATCAAACACTTGAG
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=268,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.780
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_04050 Ps_contig_mira-and-tgicl-ass_franssen_31954
SEQUENCE=AAGAATATAACAATCAACAAACATCATCTTCTCCGAGTATAGATCTGCAGCATCACCACCGTGAACATGGCGTTTCTCCGCTTTCTTCCACAACCACTCAATTCATCATTATCTACTGATTTCCTTCATCATCATCACCGTCTTCCCTATCCTCAACAACAACAACAACATCGCCTCAATTTCGCCGTCGTAAAAACCACTGCTCTCTCAGATTCTCATCCGTCTAGAACCTTACTTGATTCGAAAACTCAAGTGTTTGATGCTGTTCCTCTTTTATCTTCCTCCCAGGCCTATGAAAGGATGAAAGCATTCAGAGAAAATATAAAAGGAAAGCAGCAATATCTTGCCATGTATTCTAGTATTTTTGGTGGGATAACAACTGATCCAGCAGTTATGATTATTCCTATGGATGACCACATGGTCCATAGGGGACATGGTGTCTTTGATACTGCAGCAATAATGGACGGGTATCTGTATGAGTTAGATCAACATCTTGATCGCTTTTTAAATTCAGCATCAAGATCGAAAATAGATCTCCCGTTTGATCGTGAAAGTATTAGAAGAATTCTTATACAAACTGTGAGCGCTTCCAAATGTCGAAATGGATCACTAAGATATTGGTTATCAGCAGGACCTGGTGATTTTAACCTCTCTCCCTCTGGCTGCCACCAGTCAGTGCTTTATGCAATTGTAATACAGGACATGTCACCGGCAGCGGGCGCAGTTAAGTCCAGGGGAGTTAAAGTCATTACTTCATCTATTCCAATAAAACATCCCAAGTTTGCAATCACTAAGAGTGTGAATTACCTTCCAAATGTGCTTTCAAAGATGGAGGCAGAAGAAGCTGGTGCTTTTGCAGGGATTTGGCTTGACGATGAAGGTTTTGTTGCTGAAGGGCCTAATATGAATGTGGCTTTTGTCACTAAAGAGAAGGAACTTATAATGCCTCACTTTGACAAAATTCTAAGTGGGTGTACAGCTAAGAGAGTTTTAACCCTCGCTGAGCGTTTGATCGAGAAGGGTAAGCTTCGGGGGATAAGAAAGAAAAATGTGACCGTCGAAGAAGGGAAGAAAGCAGATGAAATGATGCTTCTTGGTAGCGGAGTTCTAGTTTGCCCTGTAGTGCAGTGGGATGAGAAGATTTTTGGAGATGGCAAGCCAGGTGATATAACAGAGGATCTCTTAAATCTAATTGTTGAAGACATGAAATCATCTCCCTCTATTCGTACATCTGTTCTATATTAACCCAACTTTATTTCCTTCTTAAGATGTGATTATGAACATTCATAAGTAGTTGTATCCTCAAAATTACCTACACTTTTGTTTCCCTGAACTTTCCATTTTGGCTCATTTGCACATTTGGAGATCGTGAAGTCCATTTGATCTGAATTTTGTAGCTAGGTTTGTATAACAATGTATGATGGTTGTATACCAAATTTGAAGTTCAAATTTTTTCCTTCACAATTAGGACTATTATTATGATATTACTAAAGTATTATATTTTGATTATAATATCTAATTTATATGATACG
TARGET=154,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1243
PRIMER_LEFT_PENALTY=0.110392
PRIMER_RIGHT_PENALTY=0.013872
PRIMER_LEFT_SEQUENCE=TCAACAAACATCATCTTCTCCG
PRIMER_RIGHT_SEQUENCE=ACGGATGAGAATCTGAGAGAGC
PRIMER_LEFT=13,22
PRIMER_RIGHT=222,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.986
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.2735
PRIMER_LEFT_1_PENALTY=0.163658
PRIMER_RIGHT_1_PENALTY=0.109882
PRIMER_LEFT_1_SEQUENCE=TTCCACAACCACTCAATTCATC
PRIMER_RIGHT_1_SEQUENCE=GCAAGATATTGCTGCTTTCCTT
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=346,22
PRIMER_LEFT_1_TM=59.836
PRIMER_RIGHT_1_TM=59.890
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.3303
PRIMER_LEFT_2_PENALTY=0.110392
PRIMER_RIGHT_2_PENALTY=0.219884
PRIMER_LEFT_2_SEQUENCE=TCAACAAACATCATCTTCTCCG
PRIMER_RIGHT_2_SEQUENCE=GAGGAACAGCATCAAACACTTG
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.780
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.3303
PRIMER_LEFT_3_PENALTY=0.110392
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=CAACAAACATCATCTTCTCCGA
PRIMER_RIGHT_3_SEQUENCE=GAGGAACAGCATCAAACACTTG
PRIMER_LEFT_3=14,22
PRIMER_RIGHT_3=270,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.3303
PRIMER_LEFT_4_PENALTY=0.110392
PRIMER_RIGHT_4_PENALTY=0.219884
PRIMER_LEFT_4_SEQUENCE=TCAACAAACATCATCTTCTCCG
PRIMER_RIGHT_4_SEQUENCE=GGAACAGCATCAAACACTTGAG
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=268,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.780
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_04051 Ps_contig_mira-and-tgicl-ass_franssen_31957
SEQUENCE=ACTTTTAGTAATACCTATAAAGAACAAACCTTTTATTATTTCTTGACTTATAAATATTTAATAAGTAATGCCATCATAATTTTTCAAGGACAAACAATACATCTTCTTACTTTTCAAAATGCATCCTTAAACAAATAAAACATATTAATTAATTATAATTTGCATGTGTATTATGGTATATGCTTCTACTCCTTAAACAGTTTTGAAGCAGCACCTTTGTCCAAGTACCATTTTAATTCCCCTTCATAAGGTGAAACCTTTTCAACAGGCAACTGAACAGTTTTCACATCATCCTCAAGTGCAGTGTAAACTGCTGTTGATTTACCCGCGCCGGTAACCACCATTGCTACATTGGAAGAAGCATTGATCACTGGAAATGTGAAAGTGATTCTCTCTGGTGGTTGTTTTGGTGAGTCATTGAGGAAAGAAACCCATTTCTGATCCTCATTGAGAAGAGGATGACCTGGGAATAAAGATGCAACATGTCCATCCGGACCCATACCTAGAAGCATGAGATCAAATTTTGGTAACCCATTGGTTGATGTGGCTATCACATTGCTAGTAACCAAGCGTCTAAGGGTTGTCTCATAAACATCTGCTGCTCCATCAGGTGGTAGGGAATCATCAATAGAATAAACATTGAGAGGGGGAATTGGCACCTTGGAGAGAAATCCATCATTGGCAAGCTTATAATTACTTTCTAAGTTATCCTTTGGGACAACCCTCTCATCAACCCAGAAAACATGCCATTTTGACCAATCTATGGTTTCAGCATAGGGAGAATCAACCAATTTCCTAAGGTACTTGATCAAAGATCCACCAGACAAAGCAACAGTGAAAGCACCTCTTTCTTTGGTAAACTTGGTAGAAAGTTGAGCAACATCATAGGCCAAGGACACAGCAAGATGTTCCTTACTGAAAACCTCAACATTCTTATAACCCTTTTCCCACTTAAGTGATGCCTCTATTTTCCCCACACATCTATTGGGTGAAAACACTTTATTATTCCTCCTTAGAGGTTGATAAACAATTTTGTTCCCAACTTGTGTTGGTAAAATTGATGTTCTTAGTGATAGTGGATTTGGTTTTTGTGCACAAAATAAACTACTTTGGAGAGTGCATGAATTAGACAAAGTTGTTAAGAATGACATTCTTGGTATTTTAATAATTTTTTTGAATATACTATGCCNCGAC
TARGET=144,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04052 Ps_contig_mira-and-tgicl-ass_franssen_31963
SEQUENCE=AGTGGTATCAACGCAGAGTCTGAGATAGGAACTTCGCAACACATTATCGATGGTAATAATGGTAATGCTGATATTGAAAATAGGGTGGTAGTAGAAGAAGAGGAAAGAGTTGCTTTGCAGATGATTGAAGAGTTACTTAATTGGAACTATCCATGTGGTTCAACTTCAACCGATTAACTCTCGGCACGAAGAAAATAGGAACATTCCATGTCATGTCATGTGGTTCTTAAAAATTTAGCAACTCCATGCCATGCCATGCCATTCTCAGCTATCTTTTCATGTACTTTTGTGTTTTCTGTAAAATATGATATGTTGTTAAAGTGCTGATAAATAGATAATAAGCACTTTGATAGTTACTTAATATAGTATATAATATATTGTGGTTGGGAAACATGAGAATTTGTATCAAATTCAGAAGAAGGATGTGTTGGAATTATTGAAAGTTCTATGAAAAGCAAATTAATCTGTCANTAAAAGT
TARGET=207,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1826
PRIMER_LEFT_PENALTY=0.087031
PRIMER_RIGHT_PENALTY=0.095574
PRIMER_LEFT_SEQUENCE=ACTCTCGGCACGAAGAAAATAG
PRIMER_RIGHT_SEQUENCE=AATTCTCATGTTTCCCAACCAC
PRIMER_LEFT=176,22
PRIMER_RIGHT=400,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.2065
PRIMER_LEFT_1_PENALTY=0.110942
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=GTTGCTTTGCAGATGATTGAAG
PRIMER_RIGHT_1_SEQUENCE=AATTCTCATGTTTCCCAACCAC
PRIMER_LEFT_1=108,22
PRIMER_RIGHT_1=400,22
PRIMER_LEFT_1_TM=59.889
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.3521
PRIMER_LEFT_2_PENALTY=0.256503
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=AACTCTCGGCACGAAGAAAATA
PRIMER_RIGHT_2_SEQUENCE=AATTCTCATGTTTCCCAACCAC
PRIMER_LEFT_2=175,22
PRIMER_RIGHT_2=400,22
PRIMER_LEFT_2_TM=60.257
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.3521
PRIMER_LEFT_3_PENALTY=0.256503
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=TAACTCTCGGCACGAAGAAAAT
PRIMER_RIGHT_3_SEQUENCE=AATTCTCATGTTTCCCAACCAC
PRIMER_LEFT_3=174,22
PRIMER_RIGHT_3=400,22
PRIMER_LEFT_3_TM=60.257
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.3521
PRIMER_LEFT_4_PENALTY=0.256503
PRIMER_RIGHT_4_PENALTY=0.095574
PRIMER_LEFT_4_SEQUENCE=ATTAACTCTCGGCACGAAGAAA
PRIMER_RIGHT_4_SEQUENCE=AATTCTCATGTTTCCCAACCAC
PRIMER_LEFT_4=172,22
PRIMER_RIGHT_4=400,22
PRIMER_LEFT_4_TM=60.257
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_04053 Ps_contig_mira-and-tgicl-ass_franssen_31963
SEQUENCE=AGTGGTATCAACGCAGAGTCTGAGATAGGAACTTCGCAACACATTATCGATGGTAATAATGGTAATGCTGATATTGAAAATAGGGTGGTAGTAGAAGAAGAGGAAAGAGTTGCTTTGCAGATGATTGAAGAGTTACTTAATTGGAACTATCCATGTGGTTCAACTTCAACCGATTAACTCTCGGCACGAAGAAAATAGGAACATTCCATGTCATGTCATGTGGTTCTTAAAAATTTAGCAACTCCATGCCATGCCATGCCATTCTCAGCTATCTTTTCATGTACTTTTGTGTTTTCTGTAAAATATGATATGTTGTTAAAGTGCTGATAAATAGATAATAAGCACTTTGATAGTTACTTAATATAGTATATAATATATTGTGGTTGGGAAACATGAGAATTTGTATCAAATTCAGAAGAAGGATGTGTTGGAATTATTGAAAGTTCTATGAAAAGCAAATTAATCTGTCANTAAAAGT
TARGET=244,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1826
PRIMER_LEFT_PENALTY=0.087031
PRIMER_RIGHT_PENALTY=0.095574
PRIMER_LEFT_SEQUENCE=ACTCTCGGCACGAAGAAAATAG
PRIMER_RIGHT_SEQUENCE=AATTCTCATGTTTCCCAACCAC
PRIMER_LEFT=176,22
PRIMER_RIGHT=400,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.2065
PRIMER_LEFT_1_PENALTY=0.110942
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=GTTGCTTTGCAGATGATTGAAG
PRIMER_RIGHT_1_SEQUENCE=AATTCTCATGTTTCCCAACCAC
PRIMER_LEFT_1=108,22
PRIMER_RIGHT_1=400,22
PRIMER_LEFT_1_TM=59.889
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.3521
PRIMER_LEFT_2_PENALTY=0.256503
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=AACTCTCGGCACGAAGAAAATA
PRIMER_RIGHT_2_SEQUENCE=AATTCTCATGTTTCCCAACCAC
PRIMER_LEFT_2=175,22
PRIMER_RIGHT_2=400,22
PRIMER_LEFT_2_TM=60.257
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.3521
PRIMER_LEFT_3_PENALTY=0.256503
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=TAACTCTCGGCACGAAGAAAAT
PRIMER_RIGHT_3_SEQUENCE=AATTCTCATGTTTCCCAACCAC
PRIMER_LEFT_3=174,22
PRIMER_RIGHT_3=400,22
PRIMER_LEFT_3_TM=60.257
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.3521
PRIMER_LEFT_4_PENALTY=0.256503
PRIMER_RIGHT_4_PENALTY=0.095574
PRIMER_LEFT_4_SEQUENCE=ATTAACTCTCGGCACGAAGAAA
PRIMER_RIGHT_4_SEQUENCE=AATTCTCATGTTTCCCAACCAC
PRIMER_LEFT_4=172,22
PRIMER_RIGHT_4=400,22
PRIMER_LEFT_4_TM=60.257
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_04054 Ps_contig_mira-and-tgicl-ass_franssen_31964
SEQUENCE=TTAGGTAACTTTCGTCTAACTACATTATCGATGGTAATAATGGTAATGCTGATATTGAAATAGGGTGGTAGTAGAAGAAGAGGAAAGAGTTGCTTTGCAGATGATTGAAGAGTTACTTAATTGGAACTATCCATGTGGTTCAACTTCAACCGATTAACTCTCGGCAACGAAGAAAATGGAACNTTCCATGTCATGTCATGTGTTACTTAAAATTG
TARGET=187,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04055 Ps_contig_mira-and-tgicl-ass_franssen_31966
SEQUENCE=CAACAGTTAAAAATTTCTCTATTATATTATTTTAATAACTATCGGGACATAATTTATAAGGATTACAATGAAAGGTAGATGCACTGTTGCTACAAATGATGGTATAAGTTTTTTTCTTTACAGACGCCATTAACAAATTTTTGTAAAGAGGGTGCAGATAAAATTGTGGAAGTCAGTACATTCTCGCTGCCTTTGAACAGAACATATACTGCACTTCAAACATCTATTGTACAATTTCAAACACGGTTACTGCAGCAAAAACGAGAAAGAGAACACCTCCAATGTAGGCAATAACCTTCTCCGACAAAAATGTCCCAAGTAAAGTACCACCAAGTACGGCTACCAAAGTTGCAACACCATGACCGGCCAGTGATCCAGCTATGACTCCCAGGGGTGAAGAAGCTGCTGCAAGGGCAATTGTAGAGAAAAATGATTTATCACCCCACTCAGCAACAAATACTAAGAGAAAAGTGCTGGCAATTGTGCTAGCAGCAGCTACTATCCCAGCACCATTTCCAGAAAATTCAGAAACTGCCAACTCTGCCTCCTTCTGTTCGTCATCTGATTTTTGGCTATCACTAGACGAGGCATCCAGCAGGGTAGAGACCCCAAAGTACACCAATAGGCAAACAGCAGCAATATCATCAATAGGTAAATCAGTTTGTCCAAACCTGAAAGGAAGGAGTTCATCAACATAATGAAAAGTCCGTCCAAGAGCGACAGATATGACAGTCATTGCCGCAAGTGCGCCAAATGTCCCAACGAAAACAACACCGGCTGAATTCCTAGCTGCTAACAATGCTGCAATGAAAAAGGTCTTGTCTCCCAATTCAGAGAAAAATATCAGCAGAAACGCTGAAGCAAAACCTGTGCTTAAGTCACCGACATCTCCAAATATAGGAATTGAATTCAAACCAGTAGCAAAATCAGAAGCAGCAGCAGCGGGATAAGAATCTCGAAGTGTGAGGAACGCGAAGAACAGCATAAACTTCACAATTCCAATAGATCCACTATCAGCATCGGAAGAAACAATCGGTGTTGCCAATTGACATCCACAATCGGAGTTTTCGGCAGTGGAGTCATCGGAAGAAGTCGCGAATCCCTTGGTGTCCCTTAGAATGCGTGGTGGTAGATTGAGATTGCAGCGAAGAGGAGGAGAAGAGAGAAGTGAGGTGGAGAAAGATGAAGATTGGAGAGTGAAGATGGTGTTAGAACGAAAGATATAAGAAGAGGATGATGCGATTGGGAATGGTTTCGGAAACGAATGGGGTGAATAGTAATGTGATTGCATTTCTAATTCTTCTTCTGAGTTGAGTTTCTACTCTGCTAT
TARGET=932,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1972
PRIMER_LEFT_PENALTY=0.197140
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=TGTGCTTAAGTCACCGACATCT
PRIMER_RIGHT_SEQUENCE=TGCAATCTCAATCTACCACCAC
PRIMER_LEFT=868,22
PRIMER_RIGHT=1143,22
PRIMER_LEFT_TM=59.803
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.1972
PRIMER_LEFT_1_PENALTY=0.197140
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=CTGTGCTTAAGTCACCGACATC
PRIMER_RIGHT_1_SEQUENCE=TGCAATCTCAATCTACCACCAC
PRIMER_LEFT_1=867,22
PRIMER_RIGHT_1=1143,22
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=277
PRIMER_PAIR_PENALTY_2=0.2150
PRIMER_LEFT_2_PENALTY=0.197140
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=CTGTGCTTAAGTCACCGACATC
PRIMER_RIGHT_2_SEQUENCE=CAATCTACCACCACGCATTCTA
PRIMER_LEFT_2=867,22
PRIMER_RIGHT_2=1135,22
PRIMER_LEFT_2_TM=59.803
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=0.2150
PRIMER_LEFT_3_PENALTY=0.197140
PRIMER_RIGHT_3_PENALTY=0.017846
PRIMER_LEFT_3_SEQUENCE=TGTGCTTAAGTCACCGACATCT
PRIMER_RIGHT_3_SEQUENCE=CAATCTACCACCACGCATTCTA
PRIMER_LEFT_3=868,22
PRIMER_RIGHT_3=1135,22
PRIMER_LEFT_3_TM=59.803
PRIMER_RIGHT_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.2724
PRIMER_LEFT_4_PENALTY=0.197140
PRIMER_RIGHT_4_PENALTY=0.075241
PRIMER_LEFT_4_SEQUENCE=TGTGCTTAAGTCACCGACATCT
PRIMER_RIGHT_4_SEQUENCE=ATTCTAAGGGACACCAAGGGAT
PRIMER_LEFT_4=868,22
PRIMER_RIGHT_4=1119,22
PRIMER_LEFT_4_TM=59.803
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_04056 Ps_contig_mira-and-tgicl-ass_franssen_31967
SEQUENCE=ATACAAAGTCACCTCACTCGACTAAAAAAAATCTAGAGATACTGCCATGGCCACACGTTCATAAGAAAAAGAAAAAAGAGAGAGTGAAACTTGTAATAGAGTAAGGAAAAGCTTTTGCGAAAAGAGTCAGAATAATCAACATTCATCATCATCATCATCATCATCTAGAAATGGCCACTATATCATCATCTTTGCCAACAAATAGTAAAACCATTTTCTCAGTATCAGCAAGACCACACTTTCTTGTTCCTTCTTCTTCTTCTTCTTCTCTTCCTTTCATTTCCACAGCAACTGTCATCCATTTCCATAATCATTCACGTGTCAATAAGCTTCACCTTCGGCAACGGTTTAATTCTGTTACAAAATTGTGTGTGGTTCCACTGCCAACAACAACTGAATGGGTTCAACAAGATCTTCCACCACCCCTTACTTCAACTTCACCTTCACCTTCTTTATTCGATGGAACTACCAGATTGTATATCTCCTACAAATGTCCCTACGCACAGCGTGTATGGATCACGCGTAACACTAAGGGATTACAGGAAAAGATACAGTTAGTTCCCATTGATTTACAAGACAGACCTTCCTGGTATAAGGACAAACTCTACCCACCAAACAAGGTTCCATCTCTTGAACATAACAATGAAGTTAGAGGAGAGAGTCTTGATCTGATCAAATATATTGACGCTCACTTTGAAGGACCTACCCTTTTTCCTAATGGTTCTCCTGATAAGGAATTTGCTGAAGAAATGATATCTTACACAGATACCTTTTCCAAGACAGTTGTTTCTTCTTTTAAAGGAGATGTAACTGAGGCTGGCACTGCTTTTGATTACCTTGAGAATGTTCTATCCAAGTATGATCATGGACCTTTCTTCCTTGGCCAATTCAGTTTAGTAGATATAGCATATGCTCCTTTTATTGAAAGATTTCAACCTTTTCTAATGGATGTGAAAAATTATGACATTACATTGGGCAGACCTAAACTGGCAGCATGGATCGAGGGAATAAACAACATTGAAGGCTACAAAATAACCCGTTCTGATCCCAAAGAGCTTGTAGAAAGCTACAAGAAGCGCTTCCAGGTCAATACATGAATACAATGTTTGGCATCATAGTACGAGTCAAGAGTATCAAGATTGATCAGAAAATCAGTATCTAAGTGATAGTAAATTTCAAGATTGTTTAGAAGATCAGAAAATCAAGATTGTTAGGACATTTGTAGTAGTAAATTTCACTGAATAATGAAAGTTTGTCTGAATTATTTGAGTTGATCTACTAGCATACACACTTGTGAGAAGTGTTTTAAGTGTTTTAAGTGTTTCCGTTGTAAATACTTGATCAAACTGATTATCCAAACATAGTCTAACTGAAAAGAAACAAGTTCAACTTCCTGATATACCATA
TARGET=143,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3361
PRIMER_LEFT_PENALTY=0.182061
PRIMER_RIGHT_PENALTY=0.154023
PRIMER_LEFT_SEQUENCE=AAGCTTTTGCGAAAAGAGTCAG
PRIMER_RIGHT_SEQUENCE=GACAGTTGCTGTGGAAATGAAA
PRIMER_LEFT=108,22
PRIMER_RIGHT=295,22
PRIMER_LEFT_TM=60.182
PRIMER_RIGHT_TM=60.154
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.3529
PRIMER_LEFT_1_PENALTY=0.182061
PRIMER_RIGHT_1_PENALTY=0.170825
PRIMER_LEFT_1_SEQUENCE=AAGCTTTTGCGAAAAGAGTCAG
PRIMER_RIGHT_1_SEQUENCE=TATGGAAATGGATGACAGTTGC
PRIMER_LEFT_1=108,22
PRIMER_RIGHT_1=308,22
PRIMER_LEFT_1_TM=60.182
PRIMER_RIGHT_1_TM=59.829
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.4236
PRIMER_LEFT_2_PENALTY=0.182061
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=AAGCTTTTGCGAAAAGAGTCAG
PRIMER_RIGHT_2_SEQUENCE=GCTGTGGAAATGAAAGGAAGAG
PRIMER_LEFT_2=108,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=60.182
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=181
PRIMER_PAIR_PENALTY_3=0.7386
PRIMER_LEFT_3_PENALTY=0.182061
PRIMER_RIGHT_3_PENALTY=0.556510
PRIMER_LEFT_3_SEQUENCE=AAGCTTTTGCGAAAAGAGTCAG
PRIMER_RIGHT_3_SEQUENCE=ACAAGAAAGTGTGGTCTTGCTG
PRIMER_LEFT_3=108,22
PRIMER_RIGHT_3=246,22
PRIMER_LEFT_3_TM=60.182
PRIMER_RIGHT_3_TM=59.443
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=0.7414
PRIMER_LEFT_4_PENALTY=0.182061
PRIMER_RIGHT_4_PENALTY=0.559375
PRIMER_LEFT_4_SEQUENCE=AAGCTTTTGCGAAAAGAGTCAG
PRIMER_RIGHT_4_SEQUENCE=ATGACAGTTGCTGTGGAAATGA
PRIMER_LEFT_4=108,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=60.182
PRIMER_RIGHT_4_TM=60.559
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_04057 Ps_contig_mira-and-tgicl-ass_franssen_31967
SEQUENCE=ATACAAAGTCACCTCACTCGACTAAAAAAAATCTAGAGATACTGCCATGGCCACACGTTCATAAGAAAAAGAAAAAAGAGAGAGTGAAACTTGTAATAGAGTAAGGAAAAGCTTTTGCGAAAAGAGTCAGAATAATCAACATTCATCATCATCATCATCATCATCTAGAAATGGCCACTATATCATCATCTTTGCCAACAAATAGTAAAACCATTTTCTCAGTATCAGCAAGACCACACTTTCTTGTTCCTTCTTCTTCTTCTTCTTCTCTTCCTTTCATTTCCACAGCAACTGTCATCCATTTCCATAATCATTCACGTGTCAATAAGCTTCACCTTCGGCAACGGTTTAATTCTGTTACAAAATTGTGTGTGGTTCCACTGCCAACAACAACTGAATGGGTTCAACAAGATCTTCCACCACCCCTTACTTCAACTTCACCTTCACCTTCTTTATTCGATGGAACTACCAGATTGTATATCTCCTACAAATGTCCCTACGCACAGCGTGTATGGATCACGCGTAACACTAAGGGATTACAGGAAAAGATACAGTTAGTTCCCATTGATTTACAAGACAGACCTTCCTGGTATAAGGACAAACTCTACCCACCAAACAAGGTTCCATCTCTTGAACATAACAATGAAGTTAGAGGAGAGAGTCTTGATCTGATCAAATATATTGACGCTCACTTTGAAGGACCTACCCTTTTTCCTAATGGTTCTCCTGATAAGGAATTTGCTGAAGAAATGATATCTTACACAGATACCTTTTCCAAGACAGTTGTTTCTTCTTTTAAAGGAGATGTAACTGAGGCTGGCACTGCTTTTGATTACCTTGAGAATGTTCTATCCAAGTATGATCATGGACCTTTCTTCCTTGGCCAATTCAGTTTAGTAGATATAGCATATGCTCCTTTTATTGAAAGATTTCAACCTTTTCTAATGGATGTGAAAAATTATGACATTACATTGGGCAGACCTAAACTGGCAGCATGGATCGAGGGAATAAACAACATTGAAGGCTACAAAATAACCCGTTCTGATCCCAAAGAGCTTGTAGAAAGCTACAAGAAGCGCTTCCAGGTCAATACATGAATACAATGTTTGGCATCATAGTACGAGTCAAGAGTATCAAGATTGATCAGAAAATCAGTATCTAAGTGATAGTAAATTTCAAGATTGTTTAGAAGATCAGAAAATCAAGATTGTTAGGACATTTGTAGTAGTAAATTTCACTGAATAATGAAAGTTTGTCTGAATTATTTGAGTTGATCTACTAGCATACACACTTGTGAGAAGTGTTTTAAGTGTTTTAAGTGTTTCCGTTGTAAATACTTGATCAAACTGATTATCCAAACATAGTCTAACTGAAAAGAAACAAGTTCAACTTCCTGATATACCATA
TARGET=250,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3361
PRIMER_LEFT_PENALTY=0.182061
PRIMER_RIGHT_PENALTY=0.154023
PRIMER_LEFT_SEQUENCE=AAGCTTTTGCGAAAAGAGTCAG
PRIMER_RIGHT_SEQUENCE=GACAGTTGCTGTGGAAATGAAA
PRIMER_LEFT=108,22
PRIMER_RIGHT=295,22
PRIMER_LEFT_TM=60.182
PRIMER_RIGHT_TM=60.154
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.3529
PRIMER_LEFT_1_PENALTY=0.182061
PRIMER_RIGHT_1_PENALTY=0.170825
PRIMER_LEFT_1_SEQUENCE=AAGCTTTTGCGAAAAGAGTCAG
PRIMER_RIGHT_1_SEQUENCE=TATGGAAATGGATGACAGTTGC
PRIMER_LEFT_1=108,22
PRIMER_RIGHT_1=308,22
PRIMER_LEFT_1_TM=60.182
PRIMER_RIGHT_1_TM=59.829
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.4737
PRIMER_LEFT_2_PENALTY=0.465411
PRIMER_RIGHT_2_PENALTY=0.008257
PRIMER_LEFT_2_SEQUENCE=TCAGCAAGACCACACTTTCTTG
PRIMER_RIGHT_2_SEQUENCE=TCTTGTTGAACCCATTCAGTTG
PRIMER_LEFT_2=224,22
PRIMER_RIGHT_2=411,22
PRIMER_LEFT_2_TM=60.465
PRIMER_RIGHT_2_TM=60.008
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.5648
PRIMER_LEFT_3_PENALTY=0.556510
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=CAGCAAGACCACACTTTCTTGT
PRIMER_RIGHT_3_SEQUENCE=TCTTGTTGAACCCATTCAGTTG
PRIMER_LEFT_3=225,22
PRIMER_RIGHT_3=411,22
PRIMER_LEFT_3_TM=59.443
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.6558
PRIMER_LEFT_4_PENALTY=0.556510
PRIMER_RIGHT_4_PENALTY=0.099308
PRIMER_LEFT_4_SEQUENCE=CAGCAAGACCACACTTTCTTGT
PRIMER_RIGHT_4_SEQUENCE=GGTGAAGTTGAAGTAAGGGGTG
PRIMER_LEFT_4=225,22
PRIMER_RIGHT_4=441,22
PRIMER_LEFT_4_TM=59.443
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_04058 Ps_contig_mira-and-tgicl-ass_franssen_31969
SEQUENCE=TAATTTCAGTTCGTGTATTGCTTACCAAGTATAAAATTAAAATGTTTCTAATACCCTGGAAATGAACAACATCATGTAAGTTTCTTACTCAACTAACATATCCTATGTTTCTTAAATGGTAGGAATGGTTCATCACTAGTTTAGAAACATGGCTTGAGATCAAATTGCTAGTAGGAGAGGAAAATATTACAAACAAGCATAACTATAACTGCTAAACTCCATACATGGTTGATCTGCTCGGTCCGTGCCGCCTTTCAGAATACTAGCTAGAGATGTTCATGACTTGCGAATGGAAATATCCATATGTAAATATGCCACAGACGCCGCAAAAAGCAGGAAAAAAACAGATACCAGCATCAATGGCTCAGCAAGCATGAATATAGGGTTAAAATTGTAGTATACCTGGAAAGGGATGTTATGCTCGGGAACAACATTTCTCTTTTCTAGAATCACCACAGTCCTTCCCACAATGTCAAGGTATGAATACTTGGTCTCTAGATGTTGATCTACTTGAAAAGGAATCACGGCTGACGGATTTTTGGATCCCTCTGGAAGCACAACCTTTATAATCAACTTGTCAACCACGGTCTCAGCAAGAGGGCAACCATAAGTAAAATTGAGGTATCTTCTTCCGTCCGGAGACTCAAAAAGGAAGTCTTGCAATGGTAGCCCATAACCAATGAGAAAGGTGGATTTCCAGCCTCCAAATAGAGGATATCGAGGCTCAAATTCAAGTTCTGACTTTAGAAAATCTGTACGGAGATGTGAAGAGGAAATATTGCCTATTTCATCCCGGTAGTATACTGAGTGAACCCTAGGGGGTAGTTTTGCAAGAAGACTTTTAAATGAAGAAACACCACTAGCACCTGATCTAGTTTGATATTCAACCCTTGAAAAAACACCTTTGTGTCTAGCACCAGCATGAACCAGTTTATATCGCTCTGTGATCTGTATGCTTCCCCAGTGAGATATTTCAATTTCACGTTCTAGCTCCTCGACAACGGCAAAGGGATTGTTATTTTCAAAATGAACAATTACAGGAGAATATGAATACGGGGTTTGACTGTCATATGGCCCATATCTTAGCTCTGAACCAGCTTGTTTAGTGGGCTCCAATACTGTGAATGATTCTACCCGAGCACTTGGTGTCTTGATGAAAGTTGTCTGTTCCTTGACATGATAGGGAGACAATAATATTGCACTATCATGGAAATAGACCAACTGCGGCTCTGATTGACTAATTTCTTCTGGGAAAGGTTCCAGAGAATGTGTCAATAAGTAAAGCACCTCAAGTGTTATCGATTCACCTTTGCCGAGTGGAGTTAACAAGGTTATAGCGAAAAATTTAGTTCCATTTGGTCCATCAGGTATCTCAGCGGATTTAACATCAAGAGGAACATAAGTCTTCTTCTTCTTCTTCCCGGTAGTTGCTGCAGCTTTGACTACTGCTAGATGTTCGGCTACAGCAGGTAGAAAAAGCAAGATGTACCTCTGAAGTAGGAGAAGTGCCTGTAGTTTTCAACCTTNANGTCAAGTACTACCTTAATA
TARGET=1404,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5091
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.413691
PRIMER_LEFT_SEQUENCE=AGCGGATTTAACATCAAGAGGA
PRIMER_RIGHT_SEQUENCE=GCTTTTTCTACCTGCTGTAGCC
PRIMER_LEFT=1374,22
PRIMER_RIGHT=1479,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.586
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=0.6450
PRIMER_LEFT_1_PENALTY=0.231280
PRIMER_RIGHT_1_PENALTY=0.413691
PRIMER_LEFT_1_SEQUENCE=GCGGCTCTGATTGACTAATTTC
PRIMER_RIGHT_1_SEQUENCE=GCTTTTTCTACCTGCTGTAGCC
PRIMER_LEFT_1=1223,22
PRIMER_RIGHT_1=1479,22
PRIMER_LEFT_1_TM=60.231
PRIMER_RIGHT_1_TM=59.586
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.7037
PRIMER_LEFT_2_PENALTY=0.290000
PRIMER_RIGHT_2_PENALTY=0.413691
PRIMER_LEFT_2_SEQUENCE=ACTGCGGCTCTGATTGACTAAT
PRIMER_RIGHT_2_SEQUENCE=GCTTTTTCTACCTGCTGTAGCC
PRIMER_LEFT_2=1220,22
PRIMER_RIGHT_2=1479,22
PRIMER_LEFT_2_TM=60.290
PRIMER_RIGHT_2_TM=59.586
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.8532
PRIMER_LEFT_3_PENALTY=0.439553
PRIMER_RIGHT_3_PENALTY=0.413691
PRIMER_LEFT_3_SEQUENCE=GCGGATTTAACATCAAGAGGAA
PRIMER_RIGHT_3_SEQUENCE=GCTTTTTCTACCTGCTGTAGCC
PRIMER_LEFT_3=1375,22
PRIMER_RIGHT_3=1479,22
PRIMER_LEFT_3_TM=60.440
PRIMER_RIGHT_3_TM=59.586
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.8835
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=0.788058
PRIMER_LEFT_4_SEQUENCE=AGCGGATTTAACATCAAGAGGA
PRIMER_RIGHT_4_SEQUENCE=TTTCTACCTGCTGTAGCCGAAC
PRIMER_LEFT_4=1374,22
PRIMER_RIGHT_4=1475,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=60.788
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=102
=
PRIMER_SEQUENCE_ID=SSR_04059 Ps_contig_mira-and-tgicl-ass_franssen_31972
SEQUENCE=GTACCTATAAATATGTGCTTGTCTATATGCTGGCAAACAATCTTATCAATCCTATCAAAATTCCAATTTGCTATATAAATACAACAAGAATCCACCTTCAAATTTTAAGCTCAATATTNNAATAAACTATCCACTAAGAAAGATCAACATGATCTAAATATAAAAGACCAGATTGGCATTATTCACTAATATACTCATCACCAATGAGCTTTATTCTTCAACCCTTTCATATTTGCTAAAAGTACATATGATCAACAAAATTTAGTGCTAAAGGTATCGCCGGTTGTCGAAGATCTCGGTCAATGCTTAATTCAGATTGCTTTTGTTTGGCGGTGCTATGGTGTTTGGTCATATGAGCGAGTTTTACCAATATATGATATACCTTATCCACCAATATGTATCATAGGCCTATTTGCTGTCTTCGCTATGTCAAACATACCGGCATGGGAAGGTTTCTTCCTCTTCACCGCAGCACTTATTATCTCCCTAAGCTCATGATCCTCTGCACCATTCCGCATGGGATCTCTTAAGCTTACCTCTGAAGGTCCAAACAAACAAACTTTTAAGTTTCCATCGGCTAGTAGTCGCAATCTATTGCAACCAGCACAAAAATGTTCAGTCATTGATGTGATAAATGAAACTTTTCCTTTATGGCCATCTATGGTGAAATTCTTAGCCGTTTCTGTCGGGTGATCCTTAACTCTTTTTAAGCTAGTAAACTGTTTCGCCTTACCACTGTAATAACCAACATTTCTGAGTAAGGTACAAGTTTCTTGACATTCCAAACATTTCCATCAAAAGGCATGAACTCAATGAACCGAACATTAATTGGCTTTTCACGCGTCAGCTCAACAAAGTCACAGATTTCATCGTCATTGAATCCACGCATTACAACACAATTCACCTTAACCGGATCATAACCAAGATCAACAGCAGCATTAATTGAATCCATTACTTTGTTATGCCCTTTCCGTCTCGTCATAAGCTCAAACTTTGCCGGTACAAGCGTATCTACACTAATGTTCAAAGAAGTAAGTCCACACTCTTTAAGCTTCGGAAGTTTTCTCGCAAGCACAATCCCATTCGTAGTCATACACAACGTCTCCAACCCCTTCAAACTCGACAACTCAAAACATATATCTTCAATATCCTTTCTAATAGTCGGCTCACCACCCGTCAACCGTATTTTATTCACACCAGAACTCACAAACAAACCAGCCAATCGCAGAATCTCGTCTTTCGTTAAAACCTGAGAGGTAGGAGTCAGGTCCACACCCTCAGCTGGCATACAATACTTACACCTTAAGTTACATCTCTCTGTTAAGGAAATTCTCAAATAAGTATGAATCCTTCCAAATGAATCAACCAACATATCAGAAACAGAGTTCTCTTTCGGCTTTTCTTCTGATAACAAATCACAAGATATTACTGAAGTTTCTGTAGAATAATAATGACTAGAATTTCCTTTGATTAAGGTTTGAAGATTACAAATTCTCGACTCAAATTTCACCGGAATTGAAATTGATGTGTTGAATCGAATTGCCGAATGAAGGAATTTGGAGAAGTAGCGTCTCATGATGATGGATAGAGAAATAGCGAAAGAGGTGAAGCATAGAGAGAGAGTTTTTACGTGTTGGGTTTGAAGTGTGAAGATAAAAATTCAGCGAAAGAGACAACACAATCTCGAGTTGTTNACTCAATCTATATCCATATNGTTTTTATATGGTATCAGACCTATTAAAT
TARGET=548,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0553
PRIMER_LEFT_PENALTY=0.053000
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=ATGGGAAGGTTTCTTCCTCTTC
PRIMER_RIGHT_SEQUENCE=AAGAGTTAAGGATCACCCGACA
PRIMER_LEFT=443,22
PRIMER_RIGHT=704,22
PRIMER_LEFT_TM=59.947
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=0.0553
PRIMER_LEFT_1_PENALTY=0.053000
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=ATGGGAAGGTTTCTTCCTCTTC
PRIMER_RIGHT_1_SEQUENCE=AGTTAAGGATCACCCGACAGAA
PRIMER_LEFT_1=443,22
PRIMER_RIGHT_1=701,22
PRIMER_LEFT_1_TM=59.947
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.0919
PRIMER_LEFT_2_PENALTY=0.089612
PRIMER_RIGHT_2_PENALTY=0.002310
PRIMER_LEFT_2_SEQUENCE=GCCTATTTGCTGTCTTCGCTAT
PRIMER_RIGHT_2_SEQUENCE=AAGAGTTAAGGATCACCCGACA
PRIMER_LEFT_2=406,22
PRIMER_RIGHT_2=704,22
PRIMER_LEFT_2_TM=59.910
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=0.0919
PRIMER_LEFT_3_PENALTY=0.089612
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=GCCTATTTGCTGTCTTCGCTAT
PRIMER_RIGHT_3_SEQUENCE=AGTTAAGGATCACCCGACAGAA
PRIMER_LEFT_3=406,22
PRIMER_RIGHT_3=701,22
PRIMER_LEFT_3_TM=59.910
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.2143
PRIMER_LEFT_4_PENALTY=0.053000
PRIMER_RIGHT_4_PENALTY=0.161322
PRIMER_LEFT_4_SEQUENCE=ATGGGAAGGTTTCTTCCTCTTC
PRIMER_RIGHT_4_SEQUENCE=CCATAGATGGCCATAAAGGAAA
PRIMER_LEFT_4=443,22
PRIMER_RIGHT_4=663,22
PRIMER_LEFT_4_TM=59.947
PRIMER_RIGHT_4_TM=60.161
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_04060 Ps_contig_mira-and-tgicl-ass_franssen_31972
SEQUENCE=GTACCTATAAATATGTGCTTGTCTATATGCTGGCAAACAATCTTATCAATCCTATCAAAATTCCAATTTGCTATATAAATACAACAAGAATCCACCTTCAAATTTTAAGCTCAATATTNNAATAAACTATCCACTAAGAAAGATCAACATGATCTAAATATAAAAGACCAGATTGGCATTATTCACTAATATACTCATCACCAATGAGCTTTATTCTTCAACCCTTTCATATTTGCTAAAAGTACATATGATCAACAAAATTTAGTGCTAAAGGTATCGCCGGTTGTCGAAGATCTCGGTCAATGCTTAATTCAGATTGCTTTTGTTTGGCGGTGCTATGGTGTTTGGTCATATGAGCGAGTTTTACCAATATATGATATACCTTATCCACCAATATGTATCATAGGCCTATTTGCTGTCTTCGCTATGTCAAACATACCGGCATGGGAAGGTTTCTTCCTCTTCACCGCAGCACTTATTATCTCCCTAAGCTCATGATCCTCTGCACCATTCCGCATGGGATCTCTTAAGCTTACCTCTGAAGGTCCAAACAAACAAACTTTTAAGTTTCCATCGGCTAGTAGTCGCAATCTATTGCAACCAGCACAAAAATGTTCAGTCATTGATGTGATAAATGAAACTTTTCCTTTATGGCCATCTATGGTGAAATTCTTAGCCGTTTCTGTCGGGTGATCCTTAACTCTTTTTAAGCTAGTAAACTGTTTCGCCTTACCACTGTAATAACCAACATTTCTGAGTAAGGTACAAGTTTCTTGACATTCCAAACATTTCCATCAAAAGGCATGAACTCAATGAACCGAACATTAATTGGCTTTTCACGCGTCAGCTCAACAAAGTCACAGATTTCATCGTCATTGAATCCACGCATTACAACACAATTCACCTTAACCGGATCATAACCAAGATCAACAGCAGCATTAATTGAATCCATTACTTTGTTATGCCCTTTCCGTCTCGTCATAAGCTCAAACTTTGCCGGTACAAGCGTATCTACACTAATGTTCAAAGAAGTAAGTCCACACTCTTTAAGCTTCGGAAGTTTTCTCGCAAGCACAATCCCATTCGTAGTCATACACAACGTCTCCAACCCCTTCAAACTCGACAACTCAAAACATATATCTTCAATATCCTTTCTAATAGTCGGCTCACCACCCGTCAACCGTATTTTATTCACACCAGAACTCACAAACAAACCAGCCAATCGCAGAATCTCGTCTTTCGTTAAAACCTGAGAGGTAGGAGTCAGGTCCACACCCTCAGCTGGCATACAATACTTACACCTTAAGTTACATCTCTCTGTTAAGGAAATTCTCAAATAAGTATGAATCCTTCCAAATGAATCAACCAACATATCAGAAACAGAGTTCTCTTTCGGCTTTTCTTCTGATAACAAATCACAAGATATTACTGAAGTTTCTGTAGAATAATAATGACTAGAATTTCCTTTGATTAAGGTTTGAAGATTACAAATTCTCGACTCAAATTTCACCGGAATTGAAATTGATGTGTTGAATCGAATTGCCGAATGAAGGAATTTGGAGAAGTAGCGTCTCATGATGATGGATAGAGAAATAGCGAAAGAGGTGAAGCATAGAGAGAGAGTTTTTACGTGTTGGGTTTGAAGTGTGAAGATAAAAATTCAGCGAAAGAGACAACACAATCTCGAGTTGTTNACTCAATCTATATCCATATNGTTTTTATATGGTATCAGACCTATTAAAT
TARGET=1614,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1336
PRIMER_LEFT_PENALTY=0.068302
PRIMER_RIGHT_PENALTY=0.065292
PRIMER_LEFT_SEQUENCE=CGAATGAAGGAATTTGGAGAAG
PRIMER_RIGHT_SEQUENCE=CACTTCAAACCCAACACGTAAA
PRIMER_LEFT=1543,22
PRIMER_RIGHT=1646,22
PRIMER_LEFT_TM=60.068
PRIMER_RIGHT_TM=59.935
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=0.1457
PRIMER_LEFT_1_PENALTY=0.065960
PRIMER_RIGHT_1_PENALTY=0.079714
PRIMER_LEFT_1_SEQUENCE=GCGTCTCATGATGATGGATAGA
PRIMER_RIGHT_1_SEQUENCE=GATTGTGTTGTCTCTTTCGCTG
PRIMER_LEFT_1=1567,22
PRIMER_RIGHT_1=1682,22
PRIMER_LEFT_1_TM=60.066
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=116
PRIMER_PAIR_PENALTY_2=0.1457
PRIMER_LEFT_2_PENALTY=0.065960
PRIMER_RIGHT_2_PENALTY=0.079714
PRIMER_LEFT_2_SEQUENCE=GCGTCTCATGATGATGGATAGA
PRIMER_RIGHT_2_SEQUENCE=ATTGTGTTGTCTCTTTCGCTGA
PRIMER_LEFT_2=1567,22
PRIMER_RIGHT_2=1681,22
PRIMER_LEFT_2_TM=60.066
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=115
PRIMER_PAIR_PENALTY_3=0.1457
PRIMER_LEFT_3_PENALTY=0.065960
PRIMER_RIGHT_3_PENALTY=0.079714
PRIMER_LEFT_3_SEQUENCE=GCGTCTCATGATGATGGATAGA
PRIMER_RIGHT_3_SEQUENCE=TGTGTTGTCTCTTTCGCTGAAT
PRIMER_LEFT_3=1567,22
PRIMER_RIGHT_3=1679,22
PRIMER_LEFT_3_TM=60.066
PRIMER_RIGHT_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=0.1480
PRIMER_LEFT_4_PENALTY=0.068302
PRIMER_RIGHT_4_PENALTY=0.079714
PRIMER_LEFT_4_SEQUENCE=CGAATGAAGGAATTTGGAGAAG
PRIMER_RIGHT_4_SEQUENCE=GATTGTGTTGTCTCTTTCGCTG
PRIMER_LEFT_4=1543,22
PRIMER_RIGHT_4=1682,22
PRIMER_LEFT_4_TM=60.068
PRIMER_RIGHT_4_TM=59.920
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=140
=
PRIMER_SEQUENCE_ID=SSR_04061 Ps_contig_mira-and-tgicl-ass_franssen_31975
SEQUENCE=TTAACCAAAGCAGAAAGTAAAGAAACAAATGAAGTAGAATGTGTATTAAAGCTATGCATCATATCTACCTATGAATAATAATAAAATTTACACACATATAAGAGTTATTTACTAAGAAACTTAATAAATTCACAACTGAAGAGCAGTGATAGATAGAAATGTCATACTAGTATCTAGCCAATGCAAAGCTCTCTTGTGTCCAGTTTGGCGGATCCATCACAGCACAGATATCCGATTTCTCAAAATCAGCAACCTTTTGGTGGCAATCAGGACAGTAATGGTATTTATGCCAGAGGCAATCCATGGAAGGACAAAGAAAGCAAACTCCAAGCATCATCGGCATCAAGCATCCAACGAATGCAGCCAGACTAATCTTGGATCTGACAGAGGTGAGAGAGGTGTGACCGCAGTGAGGACAATTGAAGGGAGCAGGAGTGTCTCTGTAAATAGTCTGTTGGATGGGAATACCTTTGGGATCTCCGATCAACGCGTTTGGTGGGATGCCGTTTCTTTCATAAACTGGTACTCCCACCGCCACTTCTTCTTCTTCTTTTCTACCCATATTCTTCAATTCTTCTATTCTTCGATCCCCCGCCGTACTCTGCGTTGATACC
TARGET=538,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7480
PRIMER_LEFT_PENALTY=0.014419
PRIMER_RIGHT_PENALTY=0.733533
PRIMER_LEFT_SEQUENCE=TGGATCTGACAGAGGTGAGAGA
PRIMER_RIGHT_SEQUENCE=GGGGGATCGAAGAATAGAAGAA
PRIMER_LEFT=375,22
PRIMER_RIGHT=592,22
PRIMER_LEFT_TM=59.986
PRIMER_RIGHT_TM=60.734
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=0.7999
PRIMER_LEFT_1_PENALTY=0.066394
PRIMER_RIGHT_1_PENALTY=0.733533
PRIMER_LEFT_1_SEQUENCE=ACAAAGAAAGCAAACTCCAAGC
PRIMER_RIGHT_1_SEQUENCE=GGGGGATCGAAGAATAGAAGAA
PRIMER_LEFT_1=310,22
PRIMER_RIGHT_1=592,22
PRIMER_LEFT_1_TM=59.934
PRIMER_RIGHT_1_TM=60.734
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.8352
PRIMER_LEFT_2_PENALTY=0.101680
PRIMER_RIGHT_2_PENALTY=0.733533
PRIMER_LEFT_2_SEQUENCE=AAGAAAGCAAACTCCAAGCATC
PRIMER_RIGHT_2_SEQUENCE=GGGGGATCGAAGAATAGAAGAA
PRIMER_LEFT_2=313,22
PRIMER_RIGHT_2=592,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.734
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=1.0083
PRIMER_LEFT_3_PENALTY=0.274768
PRIMER_RIGHT_3_PENALTY=0.733533
PRIMER_LEFT_3_SEQUENCE=AGGACAAAGAAAGCAAACTCCA
PRIMER_RIGHT_3_SEQUENCE=GGGGGATCGAAGAATAGAAGAA
PRIMER_LEFT_3=307,22
PRIMER_RIGHT_3=592,22
PRIMER_LEFT_3_TM=60.275
PRIMER_RIGHT_3_TM=60.734
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=1.0297
PRIMER_LEFT_4_PENALTY=0.296177
PRIMER_RIGHT_4_PENALTY=0.733533
PRIMER_LEFT_4_SEQUENCE=TCTGTTGGATGGGAATACCTTT
PRIMER_RIGHT_4_SEQUENCE=GGGGGATCGAAGAATAGAAGAA
PRIMER_LEFT_4=450,22
PRIMER_RIGHT_4=592,22
PRIMER_LEFT_4_TM=59.704
PRIMER_RIGHT_4_TM=60.734
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=143
=
PRIMER_SEQUENCE_ID=SSR_04062 Ps_contig_mira-and-tgicl-ass_franssen_31977
SEQUENCE=CAACGCAGNGTCGGGGATTCCATTTCTTTATTTCATCTTTTATTAGTTACTCATTTCTTGATTCAAATCATCATGGCTGAGGAGAAGCACCACCACCACCTTTTCCACCATGAAGATAAGTCTGGTTATGTTGAAGAGGTTGACTATAGAAAAGAGGAGAAGCACCACAAACATCTTGAGCACCTTGGTGAGCTTGGAACTGCAGCTGCTGGTGTCTATGCCTTGCATGAGAAGCATGAGTCAAAGAAAGACCCTGAACATGCTCACAAGCACAAGATAGAGGAGGAGATTGCGGCGGCTGCCGCCGTGGGATCCGGTGGTTTTACCTACCATGAACACCATGAGAAAAAAGAAGCTAAGAAAGAAGATGAAGAGGCTCATGGAAAAAAGCACCACCATCTCTTTTGATGAACATGATAATTATAATCAAGCTCAGAAAGATCACATAGGGTTTGTTATGTGCTATTTGCAGGGTATTTAAATAAAGTTTGAGCTATGATTTGGAGAGTGTTTGATTTTCTATAGTTTGTTATTTATTTGCATATATAAGATGTGTATTGTGTTTGAATTATGTTTAAACCCTTAAAGATGTGTGTAAGGAAATGTTTGAATAGTAATTCCAAGAGTTTGATCTAATAGAAAANTATATTGTTTTTAATTTANGAAGTACTTTTTAATCGAATTTTACTAATGTTATTGAAATAAGATAAATGTTAAAATAAAAAATT
TARGET=88,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3980
PRIMER_LEFT_PENALTY=0.375649
PRIMER_RIGHT_PENALTY=0.022302
PRIMER_LEFT_SEQUENCE=GTCGGGGATTCCATTTCTTTAT
PRIMER_RIGHT_SEQUENCE=CTTTGACTCATGCTTCTCATGC
PRIMER_LEFT=9,22
PRIMER_RIGHT=245,22
PRIMER_LEFT_TM=60.376
PRIMER_RIGHT_TM=60.022
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.5502
PRIMER_LEFT_1_PENALTY=0.375649
PRIMER_RIGHT_1_PENALTY=0.174518
PRIMER_LEFT_1_SEQUENCE=GTCGGGGATTCCATTTCTTTAT
PRIMER_RIGHT_1_SEQUENCE=ATGTTTGTGGTGCTTCTCCTCT
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=173,22
PRIMER_LEFT_1_TM=60.376
PRIMER_RIGHT_1_TM=60.175
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=0.5502
PRIMER_LEFT_2_PENALTY=0.375649
PRIMER_RIGHT_2_PENALTY=0.174518
PRIMER_LEFT_2_SEQUENCE=GTCGGGGATTCCATTTCTTTAT
PRIMER_RIGHT_2_SEQUENCE=AGATGTTTGTGGTGCTTCTCCT
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=175,22
PRIMER_LEFT_2_TM=60.376
PRIMER_RIGHT_2_TM=60.175
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.5809
PRIMER_LEFT_3_PENALTY=0.375649
PRIMER_RIGHT_3_PENALTY=0.205226
PRIMER_LEFT_3_SEQUENCE=GTCGGGGATTCCATTTCTTTAT
PRIMER_RIGHT_3_SEQUENCE=GTTTGTGGTGCTTCTCCTCTTT
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=171,22
PRIMER_LEFT_3_TM=60.376
PRIMER_RIGHT_3_TM=59.795
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=163
PRIMER_PAIR_PENALTY_4=0.5955
PRIMER_LEFT_4_PENALTY=0.375649
PRIMER_RIGHT_4_PENALTY=0.219884
PRIMER_LEFT_4_SEQUENCE=GTCGGGGATTCCATTTCTTTAT
PRIMER_RIGHT_4_SEQUENCE=CAAGATGTTTGTGGTGCTTCTC
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=177,22
PRIMER_LEFT_4_TM=60.376
PRIMER_RIGHT_4_TM=59.780
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=169
=
PRIMER_SEQUENCE_ID=SSR_04063 Ps_contig_mira-and-tgicl-ass_franssen_31987
SEQUENCE=ATYAACGCAGAGTACGCGGGGATTTTCAAGCGTGAGTGAGATTTTCTGCTTAAACCCTAGCCGCTTCACTCATTTCTCAGTAACTTTGAAGGCTTAGCTCAGTCTTCAAACAAAATGGCTTTCTGCAACAAGCTTGGAAACCTCTTGAGGCAGGGTGCCACTCAGAGCAGCCATGCTCCCGTTTCATCCATGCTTAACTATCTTCGCCATATGTCATCAAGCAAGCTTTTCATTGGAGGCCTTTCTTATGGAGTTGATGATCAGTCTCTTAAGGATGCATTTGCAAGCTATGGGGAGGTCGTTGAGGCAAGAGTTATCACTGATAGAGACACCGGAAGATCGAGGGGATTTGGTTTTGTCAACTTCACCAGTGACGAGTCTGCAACTTCAGCACTCTCTGCTATGGACGGACAGGATTTGAATGGGCGAAACATTCGTGTGTCTTATGCAAATGACAGACCTTCTGCACCTCGTGGTGGTGGTGGTGGTTATGGTGGTGGCTATGGGGATGGTTTTTCAAACCGTGGTGGCGGTGGCGGAGGTGGCGGTTGGTGATGCATCATTTAGTTTGATGTGTTTTCTGGTTATGTTGAGTCGTGTCTAAAAATTCTATGAACTTGTTACTTTCCAAGTGGAAAATCACTAATTAGTGAAATGTTATGGATTTTTTTGTTCACATTTTGGTGGTTATGTTTTTCTAGGTTCAATGTGTTTAACTCCTAAAATAATATAGTAGAATATGATGTGGTTTTCTATAATTCTTAATTGTCACGGTGTGTGTTCGCTGAAAATAATTCACTCGCTTTCAAAATT
TARGET=473,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04064 Ps_contig_mira-and-tgicl-ass_franssen_31991
SEQUENCE=GGCATCAAATTCATACATTGTGAAAGTTGGAGGGCAGTCGACGATCTTGTCACCAGGATCAAGCACACAACGCATGATCAAATCAATAAGCTCGTCGGCACCACATCCTACAAGAATATACTCAGATTCAAGGCCTGAATCTTGAGCGAGAGCTTCACGCAACCGGCGGCTCTCTGGATCTGGATAGACATATGGGAATCGTATTGACCCTAGGGCTTCCATTACCTCTGGAGGAGGGCCGTAAGGATTCTCATTAGCATCTAACTTGACAATATCCTCCGGCTTCCGTCCAAGACGAGAAGATAAAACCTCAAAAGGCAAAATGGGTTGATAAGGAGCCAATTTCCTAAGATGCTGTCTGATAAAGGAGTCACCAGTAACCTGAAGCGGACCACTGTTGACTTGTTCAACAGGAATGGTTGAAGCCATGGTCAGAAATTTCCTTCGATTCACTTGATGCTGCAAATTGGAGCAGAAAATGGATGAATTTTGAAAATGAATCACACCCATTAAGACCTGAATAACAATGGAACAACAAGAGAAAGTGAATCAAAATGTTTCAGACAACATGAATTTCCCAGAAACAGAAGAGAAGGAGGTACGAGGGCTGTGTTATGTCGTGTCCTGTTTGGTAGCCGCCGCCGCCGTAGGAAAAATGGAAAGATTTTAAATTAAAAAAAAAACTTTAATNTAAATAACTAACAAATTCGAAAATACAGAGAG
TARGET=635,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04065 Ps_contig_mira-and-tgicl-ass_franssen_32000
SEQUENCE=AGGTTTAAAGACTCCAGTTATTTAACATCTATAATATTCCAAATTTCTAACAAAAGGGACATCAAACAGTTCAACATTGCAGCTTTCCCAAGCTACTGAATTAACTGATACAACTATGTTCACGGAATCAGGGTTCAGTATAATCAATTTCTTCAAAATCTAATGAATTTGAAGAAAGTAAAGAGCTGGCATTAGTAACAGGTAGTTCAGTTAAGAAAGACAAATTTGCAGAGTTTTTTCTTTTGGAAACTGTATTTTCCTTCTTTTACCATCAAATCTATGCAAGCATTAAAAACTAGCTAGGCAATTTAGCTCCAACAACGGATGGAGCTTCACCTCTTGCCTGTTTCACATAGGAAACATATTCACCATAGTGCATAGTGAATAAGGTGCTTTGGTATCTGGTTAGATGTATGCTCTTCCTGAGTGACTCGGTGATTCTACTGTTGCAGCCAGCTTTACGTATCAAAGAATCGATGGCCTCTATTACAGTCCAACCTTCATGGGCAGCCACTTCAGGCAGGTATGTGGCACTTCGCCTGGTGTTATAGTCAGGGTCAGAAAATTCAATAATTATACCATGTTTTCCTACCTCCCAATCAAGGTAATCATTAGCAGTTTCATAGTTGGTAAGAATAGAGACAGTGCACTCCAGTTGAGGAAGCTCCCTAGCCTCTATAGGTGGAAACCTGCGGTCCCTAAGAGCGCTAGTTAGCGCATAATCTCTGAAGCCTTTGATCAAACCACGTGCTTCTAGAGAACCAATGCATCCACGAAGACGAGGCTCTCCACCATTTACCACTTTCTTCCATGTAACAAACAAGGGGTGTTGACCATCCTCGAAGGAAGGAGGAGGAGGTTGGTCACCATTGTAGTGAGCAACGAGTGTGTCGAAGCAGTAAACAACCATTTCTTGATTCGCCGTCACCATTTTCGATATTTTCTAGAATTGTTCAGAGGTTTTGAAATTGGAATTCTGAATCGATTTCAATCGGTTGTTGTTGAGAGGGTTTGGATTGGGAATTGAAACCCTAAATAAAGTGAGAAGCTTTCTCCTTTTTCACTTTTCTGCAAGCAACGTGCCCCTACGCAGGTTTCTAAATTATTTTTCTTTATAGTC
TARGET=848,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1160
PRIMER_LEFT_PENALTY=0.095988
PRIMER_RIGHT_PENALTY=0.019966
PRIMER_LEFT_SEQUENCE=AGAGACAGTGCACTCCAGTTGA
PRIMER_RIGHT_SEQUENCE=TTACTGCTTCGACACACTCGTT
PRIMER_LEFT=637,22
PRIMER_RIGHT=901,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.1160
PRIMER_LEFT_1_PENALTY=0.095988
PRIMER_RIGHT_1_PENALTY=0.019966
PRIMER_LEFT_1_SEQUENCE=AGAGACAGTGCACTCCAGTTGA
PRIMER_RIGHT_1_SEQUENCE=TTTACTGCTTCGACACACTCGT
PRIMER_LEFT_1=637,22
PRIMER_RIGHT_1=902,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.980
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.1160
PRIMER_LEFT_2_PENALTY=0.095988
PRIMER_RIGHT_2_PENALTY=0.019966
PRIMER_LEFT_2_SEQUENCE=AGAGACAGTGCACTCCAGTTGA
PRIMER_RIGHT_2_SEQUENCE=GTTTACTGCTTCGACACACTCG
PRIMER_LEFT_2=637,22
PRIMER_RIGHT_2=903,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=59.980
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=0.2789
PRIMER_LEFT_3_PENALTY=0.258908
PRIMER_RIGHT_3_PENALTY=0.019966
PRIMER_LEFT_3_SEQUENCE=CCACCATTTACCACTTTCTTCC
PRIMER_RIGHT_3_SEQUENCE=TTACTGCTTCGACACACTCGTT
PRIMER_LEFT_3=788,22
PRIMER_RIGHT_3=901,22
PRIMER_LEFT_3_TM=59.741
PRIMER_RIGHT_3_TM=59.980
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=0.2789
PRIMER_LEFT_4_PENALTY=0.258908
PRIMER_RIGHT_4_PENALTY=0.019966
PRIMER_LEFT_4_SEQUENCE=CCACCATTTACCACTTTCTTCC
PRIMER_RIGHT_4_SEQUENCE=TTTACTGCTTCGACACACTCGT
PRIMER_LEFT_4=788,22
PRIMER_RIGHT_4=902,22
PRIMER_LEFT_4_TM=59.741
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_04066 Ps_contig_mira-and-tgicl-ass_franssen_32001
SEQUENCE=TCCGCGCTGTTTCCACTCCTGCAAAGTCTGAAACTGCTACCGAGAAGAAGAGGAGCAAAGTTGAGATATTCAAGGAACAGAGCAATTTCATAAGGTATCCTCTTAAYGAGGATATGTTGACGGATGCGCCGAATTTAAGCGAAGCTGCTACTCAGTTGATCAAGTTTCATGGTAGTTATCAACAGTACAACAGAGACGAGCGTGGTTCGAGAACCTATTCGTTTATGATACGAACGAAGAACCCTTGTGGTAAAGTTTCAAATCAGCTTTACTTGACTATGGATGATCTTGCTGATCAGTTTGGAATTGGGACACTTCGTTTGACTACTAGGCAAACGTTTCAGCTACATGGTGTTGTTAAGAAGGATCTTAAAACGGTTATGGGGTCTATTATTAGAAATATGGGCTCGAGTCTTGGTGCGTGTGGTGACCTTAACAGGAATGTGCTTGCTCCTGCTGCTCCCATTGTGAGTAAAGATTATCTCTTTGCTCAAGAAACTGCGGAAAATATTGCTGCACTTTTGACTCCTCAATCTGGTTTTTACTATGATGTCTGGGTAGATGGGGAAAGATTTATGTCAGCAGAACCACCTGAGGTAATTCAGGCACGAAATGACAATTCTCACGGGACAAACTTCACAGATTCACCTGAGCCCATTTATGGAACTCAGTTCTTGCCGAGGAAATTCAAAATTGCAGTTACTGTGCCAACTGATAACTCCGTGGATATTCTCACAAATGATATTGGTGTTGTTGTTGTTACTGGCGATGGCGGGGAGCCTCAAGGATTCAATCTTTATGTTGGTGGGGGGATGGGAAGAACACATAGGATGGAAACCACTTTTCCCCGCTTGGCTGAACCATTGGGTTACGTACCAAAAGAGGATATTTTGTATGCAGTGAAAGCAATTGTTGTTACACAACGAGAAAATGGGAGAAGAGACGACCGCAGGTATAGTAGAATGAAATATCTGATCGATTCTTGGGGAATAGATAAGTTCAGAAATGTAGTTGAGGAATATTATGGCAAGAAGTTCGAACCTTTCCGCAGCTTGCCAGAATGGGAATTTAAAAGTTATCTTGGGTGGCATCAGCAGGGCGATGGTGGTTTATTTTGTGGGCTTCATGTTGACAATGGTCGTATTGCTGGAAAAATGAAAACGGCATTGAGGGAGGTTATTGAGAAGTATCATTTGAATGTTAGATTAACTCCAAATCAGAACCTCATATTGACTGATATTCGTGCTGCATGGAAACGTCCCATCACAACCATTCTTTCTCAGGCTGGTTTGCTGCTACCTAGATATGTAGATCCCCTTAATATAACGGCTATGGCATGCCCTGCTTTCCCATTATGTCCCCTGGCAATTACTGAAGCTGAACGTGGAATACCAAGTATTCTTAAGCGGATCCGTGACATGTTTGAGAAGGTTGGTCTGAAGTATAATGAATCTGTGGTTGTAAGGATAACTGGCTGCCCTAATGGCTGTGCTAGACCATATATGGCTGAACTTGGACTGGTTGGTGATGGCCCAAATAGCTATCAGATTTGGCTTGGAGGCAGCAGTAACCAAACATCAATAGCACGGAGTTTCATGGACAAGGTGAAGCTTCAAGACCTTGAAAAAGTTTTGGAGCCATTGTTTTATCATTGGAAGCAAAAGCGTCAATCTAAAGAATCATTTGGCGACTTCACAGTTCGACTGGGATTTGAGAAGCTTAAAGAATTTATTGAGAAATGGGAGGGTCCAGCAGTCCCACCAACACGCCACAACCTGAAGCTTTTTACCGATAAAGATACATACGAAGCAATGGATGGGTTAGCAAAGCTTCAAAACAAAAATGCTCATCAATTGGCCATGGAAGTTGTCCGCAATTATATTGCTTCCAACCTAAATGGAAAAGGTGAATGAATCATTTCATTGGTTAAGCCATTGAAGATGTATGTGATGTTGCATTTTGGTTGTGACTGGGAATGGCTACTGAGCAAAACTCTCAGTACTGTTACTGTGTGGTAACCCCGAGGATAGACTGAATTAATGTTTGGTTTTCATTACTTTTTGTATAAAACAAGCTTTCAGCTAGAGTCTTTTGGTGCTTTATTTTCATAACGAGCTTTGTTGAAGACAAAAATAAGCCTCTATTTCTTTTTGGGTTTCCAACTTCATGTATTTCTTCAATTAATCCTGTCTGGTATGATCTGTTGCTCAGTTTTGTTTTTATTCCTATAAAGTTAATCAATCTGATTAAAGAAATTT
TARGET=749,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04067 Ps_contig_mira-and-tgicl-ass_franssen_32003
SEQUENCE=TAATTAAGGAGGGAAGTATATACTAAAACGANATATTGCAGNTTCGCACNACAGTTCAAACCCCTCGATTTTAGGTCGTCACGCCAGATCTGCTGATTTAGAGCACCGTCAGCAGCCACCGTGTCCGGCGGAAGACATCTGCTTCCTGTTCGGAACCGTTATCACTCTCCAACCATCGAGCACAACACAGAATCGAAACATCTCAACACTTTGAAGCAAAAAACCCCAAATTTCACAATGCAAGAGTTAGATCAATGGAAGGAACAGATTTCTCACCTGTTGAATGTCGCAAATGATCACATTCGTGAAATTCCCACCGATCAACTTTATGCTGCTGCTGCTATCGCGGTTTTCACAACCCTACTTCTTCTCCTGCTTCGGTTTTTTAAGCGCAGCAAGCCTAACACCATTGTGTTGACGGGACTCAGTGGGAGTGGGAAAACCGTTCTCTTCTATCAGCTTAGGGATGGCTCTACACATCAAGGTACTGTTACGTCAATGGAGCCGAATGAAGACACTTTTATTCTTCATGGTGAAACAACGAAGAAGGGAAAGATAAAACCGGTTCATATTGTTGATGTTCCTGGACATTCTCGTCTTCGACCCAAATTGGATGAATACTTGCCTCAAGCAGCTGGCACAGTCTTTGTTGTTGATGCTTTGGACTTCTTACCAAATTGCCGTGCTGCCTCAGAGTACCTGTATGATCTGTTAACTAAGGGAAGTGTTGTGAGGAAGAAGATTCCACTGCTTATTCTCTGCAACAAAACCGATAAAGTCACTGCTCACACCAAAGAATTTATCTGCAGACAACTCGAGAAAGAAATCGACAAATTACGGGTATCAAGAAGTGCAGTTTCAGATGCCGACGTGACAAATGAGTTTACTCTGGGGGTGCCTGGCGAGCCATTTTCGTTTAAACAGTGCTCTAACACAGTTATAACTGCAGATGCTTCTGGTTTAACTGGAGAGATATCTCAGCTAGAAGAGTTTATCAGACAACATGTGAAGCCATAGCTGAATATTATGTTTAAACTTAGATTGTTGTTGAATTAAAGATATGATGAATGAGGTTGTTCCTTTTGGTTCTTACCTCTTAAAACTCAACACAGCCTGGACTAAAAGAAAAAGGGAGGGGAGGGACAGGGTAGGAGTCACTATGTTGGGATAAATGAAGATTTGGAAGTCATGTAATGTTGATTGGATTTAATGATTCAACTTTATGTCCCAGTACTTGCACTTTCTTTAATACTATGCACTYCAACGGTTTTAAA
TARGET=330,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04068 Ps_contig_mira-and-tgicl-ass_franssen_32006
SEQUENCE=CGGTTGGTCGTTCCAGAAAGGACAAGTGGTAGTCAACGCCAGAGTACGCGACTCATTCATTCATTTCCACTTACATTCTTCTTCTTCTTCTTCAATATGACTCATAATCACAAAAGCTTCATAACTATACTGTTATTAATCTTCACAGTATTTTCATTAGTTCAAGGACGAGTACCCTTTGCATGTGATCCAAGAAACGGTTTTACAAGAGGTTACAGATTCTGCAACACAAACATACCGATTCGGTTTAGAGTACAAGACCTTATCGGAAGACTTACATTACCTGAGAAGATAAGACTTGTTGTAAACAATGCAATTGCAGTTCCAAGACTTGGTATACAAGGCTACGAGTGGTGGTCGGAAGCATTACACGGCGTTTCTAACGTCGGTCCCGGTACTAAATTCGGTGGCGCTTTCCCCGGCGCCACTAGTTTTCCTCAAGTTATCACCACCGCTGCTTCTTTTAATCAGTCTTTGTGGCTCGAAATTGGACGGGTTGTGTCGGATGAAGCAAGAGCAATGTACAATGGTGGTGCAGCAGGGTTGACTTATTGGAGTCCTAATGTAAATATTTTCCGCGATCCACGGTGGGGCCGTGGCCAAGAGACACCCGGCGAAGACCCCACATTGGCCGCAAAATATGCGGCCAGCTACGTTCAAGGTCTACAAGGCAACGGCGCGGGTAACCGACTCAAGGTTGCCGCTTGTTGTAAACACTACACAGCTTATGATCTTGATAATTGGAATGGTGTAGACAGGTTTCATTTCAATGCCAAGGTGAGTAAACAGGATTTAGCAGACACATATGATGTACCATTCAAGGCGTGTGTGTCAGAAGGGAATGTGGCGAGTGTAATGTGTTCCTACAATCAAGTCAATGGCAAGCCCACTTGTGCTGATCCCGAACTTCTTCGCAACACCATCCGTGGCGAATGGCGCCTCAATGGGTACATAGTTTCAGACTGTGACTCGGTCGGAGTTTACTACGACACCCAACACTACACAAAAACACCGGAAGAGGCTGCAGCCGATGCGATTAAAGCAGGCTTGGATTTAGATTGTGGCCCATTTTTAGCCCTACATACTGATGGTGCTATTAGGCAAGGACTTATGTCTGAAAATGATCTTAACAATGCTTTAGCTAACCTTATTACAGTTCAAATGAGATTGGGTATGTTTGACGGAGAGCCTTCGGCCCAACCATATGGGAATTTAGGCCCAAGAGATGTTTGTTTACCTGCCCATAATGAACTTGCAGTTGAAGCAGCTAGACAGGGCATTGTTCTTCTGCAAAACAAAGGAAATGCATTGCCATTATCCCCAACACGCTTCCGCACCATAGGAGTCATTGGTCCCAATTCTGATGTTACTGTTACAATGATTGGAAACTATGCTGGTGTGGCATGTGGCTATACAACACCGTTGCAAGGTATTGCCAGATACGCGAGGACCATTCATCAGGTAGGATGTAGAGATGTGAGTTGTGGTGGGAACCAGCTATTTGGGCTGGCAGAGACAGTAGCAAGACAAGGTGATGCAACAGTATTAGTAATGGGCTTAGACCAGTCCATAGAAGCTGAATTTAGAGATAGGGTTGGGCTTCTTTTACCAGGCCATCAACAAGAGCTAGTGTCTAGAGTAGCTAGGGCTGCAAGAGGCCCAGTTATATTGGTTCTCATGTCTGGTGGGCCTATTGATGTTACATTTGCTAAAAATGATCCCAAAATTAGTGCTATTCTGTGGGTTGGTTATCCTGGTCAATCTGGAGGAACTGCTATTGCTGATGTTATCTTTGGCAGAACCAACCCAGGAGGAAGGTTACCCAATACATGGTACCCACAAAATTATGTGAGCAAAGTGCCAATGACAAACATGGACATGCGTCCAAACCCGGCAATAGGGTACCCAGGAAGAACCTATAGATTCTACAAGGGCCCAGTAGTGTTCCCATTTGGGCATGGGCTAAGTTACTCAAGATACACTCATACCTTAGCACTTGCTCCCAAACAAGTCTCAGTCCCTTATGTATCCCTCCAGGCCCAAGCCTTCGCAAACTCAACTAACAAAGCAATAAAAGTGAGTCATGCAAAATGTGATGAATTGGAAGTAGGTTTCCATGTGGATGTGAAAAATGAAGGGTCAATGGATGGAGCCCACACACTTCTAATATTCTCAAAACCACCTAATGGAGTTAAACAATTGGTGAACTTTCATAAGACTTATGTTCCTGCTGGATCAAAGACACGTGTTAAGGTTGGTGTTCATGTTTGTAACCACCTCTCTGTTGTCGATGAGTTTGGGATTCGAAGAATCCCAATGGGTGAACATGAACTTCACATCGGTGATCTAAAACATTCTATTTTGGTTCAAACTATGGACCAAATTAAACATTAAGTGTCAAACAAATTTGACACAGATACAAACAAGCTTTACAAATATTCTTATTAATGATTAATGATTCCTTAAGTGGAATTTTGAATATGTGCAAAAGTAAAATAAGGAATAGAGGTGGGGTTATATTTGTAAAATGATTTATAGTGCAAACACAATGTGTGCCATTCTTAATCCAAATGTAAAATAGAAAAATGTAGGTCACAATGTAAAATAGTCACAGTGGCAAGTGTACTATTGGCACTTTATATGATATTGAGAAAGGATTTTGAACAAAGTTGGAACTTTTTGTCACT
TARGET=53,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3042
PRIMER_LEFT_PENALTY=0.230329
PRIMER_RIGHT_PENALTY=0.073900
PRIMER_LEFT_SEQUENCE=AAGTGGTAGTCAACGCCAGAGT
PRIMER_RIGHT_SEQUENCE=AATCGGTATGTTTGTGTTGCAG
PRIMER_LEFT=23,22
PRIMER_RIGHT=242,22
PRIMER_LEFT_TM=60.230
PRIMER_RIGHT_TM=59.926
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.3042
PRIMER_LEFT_1_PENALTY=0.230329
PRIMER_RIGHT_1_PENALTY=0.073900
PRIMER_LEFT_1_SEQUENCE=AAGTGGTAGTCAACGCCAGAGT
PRIMER_RIGHT_1_SEQUENCE=CGGTATGTTTGTGTTGCAGAAT
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=239,22
PRIMER_LEFT_1_TM=60.230
PRIMER_RIGHT_1_TM=59.926
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.6216
PRIMER_LEFT_2_PENALTY=0.588441
PRIMER_RIGHT_2_PENALTY=0.033137
PRIMER_LEFT_2_SEQUENCE=AAGGACAAGTGGTAGTCAACGC
PRIMER_RIGHT_2_SEQUENCE=CAAAGGGTACTCGTCCTTGAAC
PRIMER_LEFT_2=17,22
PRIMER_RIGHT_2=180,22
PRIMER_LEFT_2_TM=60.588
PRIMER_RIGHT_2_TM=60.033
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.6585
PRIMER_LEFT_3_PENALTY=0.230329
PRIMER_RIGHT_3_PENALTY=0.428205
PRIMER_LEFT_3_SEQUENCE=AAGTGGTAGTCAACGCCAGAGT
PRIMER_RIGHT_3_SEQUENCE=ATCGGTATGTTTGTGTTGCAGA
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=241,22
PRIMER_LEFT_3_TM=60.230
PRIMER_RIGHT_3_TM=60.428
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=219
PRIMER_PAIR_PENALTY_4=0.6623
PRIMER_LEFT_4_PENALTY=0.588441
PRIMER_RIGHT_4_PENALTY=0.073900
PRIMER_LEFT_4_SEQUENCE=AAGGACAAGTGGTAGTCAACGC
PRIMER_RIGHT_4_SEQUENCE=CGGTATGTTTGTGTTGCAGAAT
PRIMER_LEFT_4=17,22
PRIMER_RIGHT_4=239,22
PRIMER_LEFT_4_TM=60.588
PRIMER_RIGHT_4_TM=59.926
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_04069 Ps_contig_mira-and-tgicl-ass_franssen_32006
SEQUENCE=CGGTTGGTCGTTCCAGAAAGGACAAGTGGTAGTCAACGCCAGAGTACGCGACTCATTCATTCATTTCCACTTACATTCTTCTTCTTCTTCTTCAATATGACTCATAATCACAAAAGCTTCATAACTATACTGTTATTAATCTTCACAGTATTTTCATTAGTTCAAGGACGAGTACCCTTTGCATGTGATCCAAGAAACGGTTTTACAAGAGGTTACAGATTCTGCAACACAAACATACCGATTCGGTTTAGAGTACAAGACCTTATCGGAAGACTTACATTACCTGAGAAGATAAGACTTGTTGTAAACAATGCAATTGCAGTTCCAAGACTTGGTATACAAGGCTACGAGTGGTGGTCGGAAGCATTACACGGCGTTTCTAACGTCGGTCCCGGTACTAAATTCGGTGGCGCTTTCCCCGGCGCCACTAGTTTTCCTCAAGTTATCACCACCGCTGCTTCTTTTAATCAGTCTTTGTGGCTCGAAATTGGACGGGTTGTGTCGGATGAAGCAAGAGCAATGTACAATGGTGGTGCAGCAGGGTTGACTTATTGGAGTCCTAATGTAAATATTTTCCGCGATCCACGGTGGGGCCGTGGCCAAGAGACACCCGGCGAAGACCCCACATTGGCCGCAAAATATGCGGCCAGCTACGTTCAAGGTCTACAAGGCAACGGCGCGGGTAACCGACTCAAGGTTGCCGCTTGTTGTAAACACTACACAGCTTATGATCTTGATAATTGGAATGGTGTAGACAGGTTTCATTTCAATGCCAAGGTGAGTAAACAGGATTTAGCAGACACATATGATGTACCATTCAAGGCGTGTGTGTCAGAAGGGAATGTGGCGAGTGTAATGTGTTCCTACAATCAAGTCAATGGCAAGCCCACTTGTGCTGATCCCGAACTTCTTCGCAACACCATCCGTGGCGAATGGCGCCTCAATGGGTACATAGTTTCAGACTGTGACTCGGTCGGAGTTTACTACGACACCCAACACTACACAAAAACACCGGAAGAGGCTGCAGCCGATGCGATTAAAGCAGGCTTGGATTTAGATTGTGGCCCATTTTTAGCCCTACATACTGATGGTGCTATTAGGCAAGGACTTATGTCTGAAAATGATCTTAACAATGCTTTAGCTAACCTTATTACAGTTCAAATGAGATTGGGTATGTTTGACGGAGAGCCTTCGGCCCAACCATATGGGAATTTAGGCCCAAGAGATGTTTGTTTACCTGCCCATAATGAACTTGCAGTTGAAGCAGCTAGACAGGGCATTGTTCTTCTGCAAAACAAAGGAAATGCATTGCCATTATCCCCAACACGCTTCCGCACCATAGGAGTCATTGGTCCCAATTCTGATGTTACTGTTACAATGATTGGAAACTATGCTGGTGTGGCATGTGGCTATACAACACCGTTGCAAGGTATTGCCAGATACGCGAGGACCATTCATCAGGTAGGATGTAGAGATGTGAGTTGTGGTGGGAACCAGCTATTTGGGCTGGCAGAGACAGTAGCAAGACAAGGTGATGCAACAGTATTAGTAATGGGCTTAGACCAGTCCATAGAAGCTGAATTTAGAGATAGGGTTGGGCTTCTTTTACCAGGCCATCAACAAGAGCTAGTGTCTAGAGTAGCTAGGGCTGCAAGAGGCCCAGTTATATTGGTTCTCATGTCTGGTGGGCCTATTGATGTTACATTTGCTAAAAATGATCCCAAAATTAGTGCTATTCTGTGGGTTGGTTATCCTGGTCAATCTGGAGGAACTGCTATTGCTGATGTTATCTTTGGCAGAACCAACCCAGGAGGAAGGTTACCCAATACATGGTACCCACAAAATTATGTGAGCAAAGTGCCAATGACAAACATGGACATGCGTCCAAACCCGGCAATAGGGTACCCAGGAAGAACCTATAGATTCTACAAGGGCCCAGTAGTGTTCCCATTTGGGCATGGGCTAAGTTACTCAAGATACACTCATACCTTAGCACTTGCTCCCAAACAAGTCTCAGTCCCTTATGTATCCCTCCAGGCCCAAGCCTTCGCAAACTCAACTAACAAAGCAATAAAAGTGAGTCATGCAAAATGTGATGAATTGGAAGTAGGTTTCCATGTGGATGTGAAAAATGAAGGGTCAATGGATGGAGCCCACACACTTCTAATATTCTCAAAACCACCTAATGGAGTTAAACAATTGGTGAACTTTCATAAGACTTATGTTCCTGCTGGATCAAAGACACGTGTTAAGGTTGGTGTTCATGTTTGTAACCACCTCTCTGTTGTCGATGAGTTTGGGATTCGAAGAATCCCAATGGGTGAACATGAACTTCACATCGGTGATCTAAAACATTCTATTTTGGTTCAAACTATGGACCAAATTAAACATTAAGTGTCAAACAAATTTGACACAGATACAAACAAGCTTTACAAATATTCTTATTAATGATTAATGATTCCTTAAGTGGAATTTTGAATATGTGCAAAAGTAAAATAAGGAATAGAGGTGGGGTTATATTTGTAAAATGATTTATAGTGCAAACACAATGTGTGCCATTCTTAATCCAAATGTAAAATAGAAAAATGTAGGTCACAATGTAAAATAGTCACAGTGGCAAGTGTACTATTGGCACTTTATATGATATTGAGAAAGGATTTTGAACAAAGTTGGAACTTTTTGTCACT
TARGET=76,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3042
PRIMER_LEFT_PENALTY=0.230329
PRIMER_RIGHT_PENALTY=0.073900
PRIMER_LEFT_SEQUENCE=AAGTGGTAGTCAACGCCAGAGT
PRIMER_RIGHT_SEQUENCE=AATCGGTATGTTTGTGTTGCAG
PRIMER_LEFT=23,22
PRIMER_RIGHT=242,22
PRIMER_LEFT_TM=60.230
PRIMER_RIGHT_TM=59.926
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.3042
PRIMER_LEFT_1_PENALTY=0.230329
PRIMER_RIGHT_1_PENALTY=0.073900
PRIMER_LEFT_1_SEQUENCE=AAGTGGTAGTCAACGCCAGAGT
PRIMER_RIGHT_1_SEQUENCE=CGGTATGTTTGTGTTGCAGAAT
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=239,22
PRIMER_LEFT_1_TM=60.230
PRIMER_RIGHT_1_TM=59.926
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.3115
PRIMER_LEFT_2_PENALTY=0.278329
PRIMER_RIGHT_2_PENALTY=0.033137
PRIMER_LEFT_2_SEQUENCE=GAGTACGCGACTCATTCATTCA
PRIMER_RIGHT_2_SEQUENCE=CAAAGGGTACTCGTCCTTGAAC
PRIMER_LEFT_2=41,22
PRIMER_RIGHT_2=180,22
PRIMER_LEFT_2_TM=60.278
PRIMER_RIGHT_2_TM=60.033
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=0.3522
PRIMER_LEFT_3_PENALTY=0.278329
PRIMER_RIGHT_3_PENALTY=0.073900
PRIMER_LEFT_3_SEQUENCE=GAGTACGCGACTCATTCATTCA
PRIMER_RIGHT_3_SEQUENCE=AATCGGTATGTTTGTGTTGCAG
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=242,22
PRIMER_LEFT_3_TM=60.278
PRIMER_RIGHT_3_TM=59.926
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=202
PRIMER_PAIR_PENALTY_4=0.5992
PRIMER_LEFT_4_PENALTY=0.566087
PRIMER_RIGHT_4_PENALTY=0.033137
PRIMER_LEFT_4_SEQUENCE=CGACTCATTCATTCATTTCCAC
PRIMER_RIGHT_4_SEQUENCE=CAAAGGGTACTCGTCCTTGAAC
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=180,22
PRIMER_LEFT_4_TM=59.434
PRIMER_RIGHT_4_TM=60.033
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_04070 Ps_contig_mira-and-tgicl-ass_franssen_32014
SEQUENCE=AACGCAGAGTACGCGGGGACACCAAAAATTTCGCTGAAAGCTACAACGTCTTTCAACAGATCCATTATCATATCCATTTTTTTTCTCTCTCTTTTCCCGTTTTCGAGTTCAAGCAATGGCGTCTGAAGAGCCCACCACCGTCGCCGTCGAGCAACCCATTGTGGAGGAGCCGGAAGCCGTCGACACGCTCCCACCAGTTGTCAATGAATCCGAAGAGCCCACTGCCAAGCCCAAGAAGGCACCCAAGGAGCCCAAGGCCAAGAAAGCTCCGGCCAAGCCCCGAACTCACCCCACCTATGAAGAGATGGTGAAGGAAGCGATTGTTGCTTTGAAGGAGAGGAACGGTTCAAGCCAGTACGCGATTGCGAAGTTCATTGAAGAGAAGCACAAGCAGCTTCCATCGAACTTCAAGAAGCTTTTGCTGGTTCAGATCAAGAAGCTTGTTGCTTCTGGCAAGCTCGTCAAGGTTAAGGCCTCTTACAAGTTACCGGCGAAGTCCTCTGCTGCAAAGCCCGCCAAGAAGCCTGCTGCTGCTGCTAAGTCCAAAGCCAAGCCCAAGGCTAAAGCTGCTACCAAGTCAAAGGCTAAGCCCGCAGCCAAAGCCAAGCCAGCTGCCAAAGCCAAGCCCGCTGCTAAGGCCAAGCCAGCTGCCAAGGCTAAGCCCGCTGCTAAGGCAAAGCCTGCTGCCAAAGCTAAACCCGCTGCCAAGCCGGTTAAGACTGCCGCAGCCAAGCCAGCAGCCAAGGCCAAGCCTGCCGCGAAGCCTAAAGCTGCTGCTAAGGCGAAACCTGCTGCCAAAGCCAAGCCAGCTGCTAAGGCTAAGCCAGCTGCTAAGCCTGCCAAGGCTGCAAGGACCTCGACGAGGACTTCACCAGCGGCTGCAGCTGCTGCACCCAAGCCGGCGGTGAAGAAGGCTGCACCTGTGAAGAAGACTCCGGTGAAAGCAGCGGCGAAGGCCAAGACTGCGAAGTCTCCAGCGAAGAAGGCAGCAGCTAAGAGAGGGAAGAAGTGAAGGTTGTAAGGGTAATTAGTGAAGCTAGTGTTTGTAGCGTTAGCTGTAAATTTTCTTTGATCTGCGGTTTAGTCACAGATGTTGTCGACAAATTCTGAATTTATTAAACTTTTTATTTTTCATCGTTTCGTACACAGTTTTCTAGCTATGTAATTTTCCCCTATTATTGAGTTTAATCATAAATCAAAGCTAGAAGGGATTTAGGGTTTCAATGTCTTGTTATAGTGATCTTTTTACAATGCAATGGATCTGTGTTTTTGCATACTTTTCTGCAGATTTTGTTTATTAGTTTTTTATGTCATCCATTGAATTGATCTGTGGTTTTGGTCATTAAAATTTTCATAGTCTTGTTATTATTTCTATTCTTCTTCTTTGTACTACCCGTATTAGTTAAACTTACCCTTAAATTTGGTTAGGTTTTAAATTAAATTTAACAAATTAAGAAAAAGT
TARGET=525,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1073
PRIMER_LEFT_PENALTY=0.055230
PRIMER_RIGHT_PENALTY=0.052069
PRIMER_LEFT_SEQUENCE=CTTCAAGAAGCTTTTGCTGGTT
PRIMER_RIGHT_SEQUENCE=CCTTTGACTTGGTAGCAGCTTT
PRIMER_LEFT=405,22
PRIMER_RIGHT=583,22
PRIMER_LEFT_TM=60.055
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.2341
PRIMER_LEFT_1_PENALTY=0.182061
PRIMER_RIGHT_1_PENALTY=0.052069
PRIMER_LEFT_1_SEQUENCE=TCTTACAAGTTACCGGCGAAGT
PRIMER_RIGHT_1_SEQUENCE=CCTTTGACTTGGTAGCAGCTTT
PRIMER_LEFT_1=475,22
PRIMER_RIGHT_1=583,22
PRIMER_LEFT_1_TM=60.182
PRIMER_RIGHT_1_TM=59.948
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.2341
PRIMER_LEFT_2_PENALTY=0.182061
PRIMER_RIGHT_2_PENALTY=0.052069
PRIMER_LEFT_2_SEQUENCE=CTTACAAGTTACCGGCGAAGTC
PRIMER_RIGHT_2_SEQUENCE=CCTTTGACTTGGTAGCAGCTTT
PRIMER_LEFT_2=476,22
PRIMER_RIGHT_2=583,22
PRIMER_LEFT_2_TM=60.182
PRIMER_RIGHT_2_TM=59.948
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.2417
PRIMER_LEFT_3_PENALTY=0.189596
PRIMER_RIGHT_3_PENALTY=0.052069
PRIMER_LEFT_3_SEQUENCE=CATTGAAGAGAAGCACAAGCAG
PRIMER_RIGHT_3_SEQUENCE=CCTTTGACTTGGTAGCAGCTTT
PRIMER_LEFT_3=372,22
PRIMER_RIGHT_3=583,22
PRIMER_LEFT_3_TM=60.190
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.2936
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=0.052069
PRIMER_LEFT_4_SEQUENCE=ATTGTTGCTTTGAAGGAGAGGA
PRIMER_RIGHT_4_SEQUENCE=CCTTTGACTTGGTAGCAGCTTT
PRIMER_LEFT_4=319,22
PRIMER_RIGHT_4=583,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=59.948
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_04071 Ps_contig_mira-and-tgicl-ass_franssen_32019
SEQUENCE=AAATTTGAAATTATTAATAAATAACAAAAATCCCTAAAAACTTACATGACAAAACTTGAACTTTATTAATTCTAAAAGTAAAATAAATAGAAAGATAAAGCAATTTTTTATGCTTGAATTAAAATAATTTACACCATGGATTTTCATCATTACCACATAGTAATTATTCAACCCCATACCAGAGCCATAATCCCAAACCATCCTTAACTGGGACAAGCAGCCTATCCCAACCATTTCTCTGTTCTTTCCTTCACGTGAACAACACATAGCCATAGTTTTGCTACCAAACCAGACATTATTTTACAAAGAAATTGAGGTTTCATTTTCCCTCTTCATCGTTTTGTTTCCATTAAGTTCAACTCCATTGATCTGGACAGTACCATTGTAACGTTGGTGCTTGGTCGTCTGAGAAAAAATTGCAGGCCTCATCGGCACTCAAGAAATTCTGTTCCTCCATTTTCACATGATAAGATTTCTGATACTGGAAGCAATTCATGGAGGAAGAATCAGGAGACAACAAAAGGTGACTTTGAAGAATTCCTCCAGATGAAGAAATTGTGCTAGAATCACTGTCAGATGAACCGTCTCTTAAGTCTGCTGGAAAGAGTGAAGAAGCTTCTCCAACACCAAAATCATTGTTGTTGTTAATGATGCTGTCATAATTCAAATGATCCTTAGATTCTAAACTAGGAATTGATGTTTCCGAAGACGGGTTATGCTTGTTTTCATCAGATTCAGGCATTGTTATCATCTCTTCTTTCACCAAAACACTGCTTGCACTTTTTTCTTCTTCTTCCAGCTTCAATTTCAATTCCTTAATCTCCTTATGAAAAGCTTTGTTGTCTTGAGTAATTGCATCTAATTTAAGCTTAAGAGCATCATAATTAGCTTTAAGAACACCATAATCTCTTTCCAACTGTTTTGTCTTCCATCTCGCACGACGGTTTTGAAACCAAACAGCAACTTGTCTAGGCTGCAACCCAAGTTCTACTGCAAGCTTCTCCTTTCTCTCGGGTTCAAGCTTGTTTTCTACTTCAAAGTTTTTCTCTAAAGCCTTCACTTGATCCACTCTCAATCTTCGTTTCTTCTCAGAGTGATGATGACCGGTTTCTTCAACAGAGACTTCCTCTTCAAATCCATCCAACATCATGGATTGAAACTCCATACCATACACATGTTTGTTCCTCGGACTATTTTCTTCTGAATGAACARAGTAAACAAAAACATAATCAGTACTCTGCGTTGATACCA
TARGET=785,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04072 Ps_contig_mira-and-tgicl-ass_franssen_32021
SEQUENCE=TGCACCGGCCGGCAGAAGGAGAAGGAGGAGGCATAGGAATCTCATCCACCAGCTCAGAAGATAATCATCCCATCTCCAACAACAACAACAACAACTCCGACACCACGTTAGAGCTCAACTCCCACATCTCCCTCCCTTACCATTGGGAACAATGCTTAGACTTAAAGACGGGGGAGATTTATTACTTAAACTGGAGGAACGGAATGAAAGCAAAGGAGGATCCGAGAATAGTAGCAGAAAGGGATTGTGAACAACAGGAGTCAGAATCAGAAGATGATGAAGAAGAAGAAGAGAGTTTGTACGAAGATAGTGAAGAATGTTCATCAGAGTGCTCAACGAATGAGAGAGGAGAGGTAATAGAGAAAGAGAATGTTCTGGTAGTGGCTGGTTGCAAGAGCTGTTTGATGTATTTCATGGTGCCAAAACAAGTTGAAGACTGCCCAAAATGTAATGGTCAACTTCTCCACTTTGATCGATCCGAAAATTGCTCCCCTTGATTATTAATTCAACCCTTTCTCTCTTTTCATTTTTTATTTCATTTTGTCTCGCATTATGAACTTTTTTCTATAATATTAATATTTTGGAAACCAAATAACTTTGTCTAGTCCGGATAATTTATAGTTGTGAAGAGACATCAATTATTTANAGGAAAATTTCAGTTTACTAAAAACCTT
TARGET=77,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0673
PRIMER_LEFT_PENALTY=0.061624
PRIMER_RIGHT_PENALTY=0.005674
PRIMER_LEFT_SEQUENCE=AAGGAGGAGGCATAGGAATCTC
PRIMER_RIGHT_SEQUENCE=CATTCCGTTCCTCCAGTTTAAG
PRIMER_LEFT=21,22
PRIMER_RIGHT=205,22
PRIMER_LEFT_TM=60.062
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=0.0673
PRIMER_LEFT_1_PENALTY=0.061624
PRIMER_RIGHT_1_PENALTY=0.005674
PRIMER_LEFT_1_SEQUENCE=GAAGGAGGAGGCATAGGAATCT
PRIMER_RIGHT_1_SEQUENCE=CATTCCGTTCCTCCAGTTTAAG
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=60.062
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=0.0673
PRIMER_LEFT_2_PENALTY=0.061624
PRIMER_RIGHT_2_PENALTY=0.005674
PRIMER_LEFT_2_SEQUENCE=GAGAAGGAGGAGGCATAGGAAT
PRIMER_RIGHT_2_SEQUENCE=CATTCCGTTCCTCCAGTTTAAG
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=205,22
PRIMER_LEFT_2_TM=60.062
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.0673
PRIMER_LEFT_3_PENALTY=0.061624
PRIMER_RIGHT_3_PENALTY=0.005674
PRIMER_LEFT_3_SEQUENCE=AGAAGGAGGAGGCATAGGAATC
PRIMER_RIGHT_3_SEQUENCE=CATTCCGTTCCTCCAGTTTAAG
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=60.062
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.0699
PRIMER_LEFT_4_PENALTY=0.061624
PRIMER_RIGHT_4_PENALTY=0.008257
PRIMER_LEFT_4_SEQUENCE=GAAGGAGGAGGCATAGGAATCT
PRIMER_RIGHT_4_SEQUENCE=TGTTGTTCACAATCCCTTTCTG
PRIMER_LEFT_4=20,22
PRIMER_RIGHT_4=255,22
PRIMER_LEFT_4_TM=60.062
PRIMER_RIGHT_4_TM=60.008
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_04073 Ps_contig_mira-and-tgicl-ass_franssen_32021
SEQUENCE=TGCACCGGCCGGCAGAAGGAGAAGGAGGAGGCATAGGAATCTCATCCACCAGCTCAGAAGATAATCATCCCATCTCCAACAACAACAACAACAACTCCGACACCACGTTAGAGCTCAACTCCCACATCTCCCTCCCTTACCATTGGGAACAATGCTTAGACTTAAAGACGGGGGAGATTTATTACTTAAACTGGAGGAACGGAATGAAAGCAAAGGAGGATCCGAGAATAGTAGCAGAAAGGGATTGTGAACAACAGGAGTCAGAATCAGAAGATGATGAAGAAGAAGAAGAGAGTTTGTACGAAGATAGTGAAGAATGTTCATCAGAGTGCTCAACGAATGAGAGAGGAGAGGTAATAGAGAAAGAGAATGTTCTGGTAGTGGCTGGTTGCAAGAGCTGTTTGATGTATTTCATGGTGCCAAAACAAGTTGAAGACTGCCCAAAATGTAATGGTCAACTTCTCCACTTTGATCGATCCGAAAATTGCTCCCCTTGATTATTAATTCAACCCTTTCTCTCTTTTCATTTTTTATTTCATTTTGTCTCGCATTATGAACTTTTTTCTATAATATTAATATTTTGGAAACCAAATAACTTTGTCTAGTCCGGATAATTTATAGTTGTGAAGAGACATCAATTATTTANAGGAAAATTTCAGTTTACTAAAAACCTT
TARGET=279,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0456
PRIMER_LEFT_PENALTY=0.005674
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=CTTAAACTGGAGGAACGGAATG
PRIMER_RIGHT_SEQUENCE=ACATTTTGGGCAGTCTTCAACT
PRIMER_LEFT=184,22
PRIMER_RIGHT=448,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.0481
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=CAGAAAGGGATTGTGAACAACA
PRIMER_RIGHT_1_SEQUENCE=ACATTTTGGGCAGTCTTCAACT
PRIMER_LEFT_1=234,22
PRIMER_RIGHT_1=448,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=215
PRIMER_PAIR_PENALTY_2=0.0523
PRIMER_LEFT_2_PENALTY=0.012470
PRIMER_RIGHT_2_PENALTY=0.039879
PRIMER_LEFT_2_SEQUENCE=GGATTGTGAACAACAGGAGTCA
PRIMER_RIGHT_2_SEQUENCE=ACATTTTGGGCAGTCTTCAACT
PRIMER_LEFT_2=241,22
PRIMER_RIGHT_2=448,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.040
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.0799
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.071684
PRIMER_LEFT_3_SEQUENCE=CAGAAAGGGATTGTGAACAACA
PRIMER_RIGHT_3_SEQUENCE=AACCAGCCACTACCAGAACATT
PRIMER_LEFT_3=234,22
PRIMER_RIGHT_3=389,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.928
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.0842
PRIMER_LEFT_4_PENALTY=0.012470
PRIMER_RIGHT_4_PENALTY=0.071684
PRIMER_LEFT_4_SEQUENCE=GGATTGTGAACAACAGGAGTCA
PRIMER_RIGHT_4_SEQUENCE=AACCAGCCACTACCAGAACATT
PRIMER_LEFT_4=241,22
PRIMER_RIGHT_4=389,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.928
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_04074 Ps_contig_mira-and-tgicl-ass_franssen_32033
SEQUENCE=ACACTTCCACAACCNCCCCGGACCACCTACCTGCAACCGTCAACCGCTCACACATTATATTTTCCTATTTCTTTCCTTTTTTCCTAATTCCCATTCTTCAACAGCGTGTGAGTCACACCACCACCTTCTCTCAAAACCCCCAAAAATGTTGTAAATATCGACCAAATTTTTCCACAAATGGAGGGTTGGTTCTCATCTTCAACCTCACCCAGTGACGATCAGCAGAAACCAGCTTCCTCCTCTTCTTCTTCTTTGCTCGCTGATTGGAACTCATACGCTACATCTCAACAGGAACAAGACCCTTCCAATTCCGCCATCAGCTTCGATCTTGAATCCGCTGTTAGATCTGCTAACAACAGTGTCACTGGCACTTTCAGCGTGGTTTCTAAAAGTGTGAGAGATATACCTGGAAACTTCTCATCTGCGACTAGCAATGTTCCTTCTGGAAAAGCTCTCCTGTATTTTGGTTTATTTCTGGCTAGCGGTGTCTTCTTCATTTTCATTGCATTTACCTTGTTCCTGCCTATCATGGTGGTTATGCCCCAAAAGTTTGCTATATGCTTTACGCTTGGGTGCGGGTTTATTATTGGATCGTTCTTTGCTCTAAAAGGGCCAAAGAATCAGCTTTCTCACATGATGTCGAAGGAGAGGCTCCCATTCACACTGGGGTTTTTATGCAGTATGGTTGGCACCATTTATGTATCTATGGTGTTACATAGCTACATTCTCTCTGTTGTATTCTCTGTTGTACAGGTTCTTTCACTTGGATACTATGCTATATCATATTTTCCTGGTGGATCTGCTGGAATGAGATTTTTGACTTCTGCTTTAACATCCAGAATAATGACTTGTTTTGGGAGGTGATTTGGTTTTGGTTATTGTTATTACTTTTTTTGGGAGGTGATAATGCTGAGATGAAACATCATTTAACATGTCCTAAGATTTCATTCAATGTTTGTTGTTATCTCTAAAAGAGAAAGTTGGTTGATCTCTTTGCCCAATGTTGCAATCGTTATTGCATGCTAATGGGTATGCATATTGGTATCTATATGGCATATGGTTGCCGAGGGTTTGACAATTTCTTTTTTGATACATAAAGATAGGGTCTTGAATGAAGTGGCTTGCCAGATTCTTGTTTANYNTGTGGCAAACCCAATAATTTTGCAGCAAATCTAGGCAGGTTTAGAATATTCTCACCAACCAAAACGTAAATGTATCTATTTTCCTCTCTGTAAACAAAAACATAAGATACCATTAAGC
TARGET=241,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04075 Ps_contig_mira-and-tgicl-ass_franssen_32037
SEQUENCE=CTTTTGTTCCTTCTCAGAGTTATCCTCCCAATCTCCGCCAACCACCATCTCAGCCACCCAGTGGACCTCCTCCACCTGCCCAGCAATTCTATGGGACACCGTCCCAAGGATATGAATCACCATCAAGTAGATCTGGTTCAAGTTATTCTTCTGGATATGGTACATTATCTGGGCCTGCTGAGCCATATCGATATGGTGGGCCACCTCAGTATGGTGGCAAACAACCACAAGTACATAACGTCTCTGTGTCTTCCAGTGGTGGAAGTGGTTACCCACAGCTTCCAACTGCCCGTGCTCTCCCACAAGCAATACCTACTGCATCATCGGTAAGTGGTGGCTCAGGCTCTGCTGGAACTGGTAACAGGGTTTCTGTTGACGATGTAGTTGAAAAAGTTGCTACAATGGGATTCCCTAGAGACCATGTGAGGGCAACGGTTCGGAAGCTGACAGAGAATGGTCAATCAGTTGACCTAAATACAGTGCTGGATAAGCTTATGAATGAAGGTGGCGGTGATATGCAGCAACAAAGAGGTTGGTTTGGTCGGTAAAATGGCGGTAATCAATTGAACTTTTGTATAGAAGTATGATTTGTTTCGTGATGAAGGTTTATGAGGAAGGATGACTTCGTGGCTGGATTGTGCCAATTGAATATTGTTCTTTTTTTTCTTTCTTTCCAATTGTTCTTAGTTCTTGGATAGTTGAAACTGTTATGCTTTCTTGTGGGTTTAATTACTATCTTAATCTTGTGCAGTATATTTTCAATAAATAAAACGGGATTTGGTAAGTTGCTCTGAAGTT
TARGET=663,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7082
PRIMER_LEFT_PENALTY=0.225403
PRIMER_RIGHT_PENALTY=0.482784
PRIMER_LEFT_SEQUENCE=TGATATGCAGCAACAAAGAGGT
PRIMER_RIGHT_SEQUENCE=TCAGAGCAACTTACCAAATCCC
PRIMER_LEFT=511,22
PRIMER_RIGHT=793,22
PRIMER_LEFT_TM=59.775
PRIMER_RIGHT_TM=60.483
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.7082
PRIMER_LEFT_1_PENALTY=0.225403
PRIMER_RIGHT_1_PENALTY=0.482784
PRIMER_LEFT_1_SEQUENCE=GGTGATATGCAGCAACAAAGAG
PRIMER_RIGHT_1_SEQUENCE=TCAGAGCAACTTACCAAATCCC
PRIMER_LEFT_1=509,22
PRIMER_RIGHT_1=793,22
PRIMER_LEFT_1_TM=59.775
PRIMER_RIGHT_1_TM=60.483
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.7082
PRIMER_LEFT_2_PENALTY=0.225403
PRIMER_RIGHT_2_PENALTY=0.482784
PRIMER_LEFT_2_SEQUENCE=GTGATATGCAGCAACAAAGAGG
PRIMER_RIGHT_2_SEQUENCE=TCAGAGCAACTTACCAAATCCC
PRIMER_LEFT_2=510,22
PRIMER_RIGHT_2=793,22
PRIMER_LEFT_2_TM=59.775
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=284
PRIMER_PAIR_PENALTY_3=1.1891
PRIMER_LEFT_3_PENALTY=0.706271
PRIMER_RIGHT_3_PENALTY=0.482784
PRIMER_LEFT_3_SEQUENCE=ATATGCAGCAACAAAGAGGTTG
PRIMER_RIGHT_3_SEQUENCE=TCAGAGCAACTTACCAAATCCC
PRIMER_LEFT_3=513,22
PRIMER_RIGHT_3=793,22
PRIMER_LEFT_3_TM=59.294
PRIMER_RIGHT_3_TM=60.483
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=1.2883
PRIMER_LEFT_4_PENALTY=0.805475
PRIMER_RIGHT_4_PENALTY=0.482784
PRIMER_LEFT_4_SEQUENCE=TATGAGGAAGGATGACTTCGTG
PRIMER_RIGHT_4_SEQUENCE=TCAGAGCAACTTACCAAATCCC
PRIMER_LEFT_4=607,22
PRIMER_RIGHT_4=793,22
PRIMER_LEFT_4_TM=59.195
PRIMER_RIGHT_4_TM=60.483
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_04076 Ps_contig_mira-and-tgicl-ass_franssen_32043
SEQUENCE=TCAACGCAGAGTACCTTCTCTTTCTACCTCTGCCTCCTTCTTAGGTGTTTTTGTTAAGATTTGTGATTTTTTTTCTTTCTCAAGAGAAGATGAATTGCTACTATGGATTCCCACAGATGAACACAATGACAAACAGCTTTGAAGAAGTGAAGATGGAATCTGTGGTTTGTCCTAAACCTCGTCGGTATCCATCCATCAACAGACCCTTCAGGCGTCCTTTTAGCTACCAATCTGAGATTGAAGATTCAGGAGTTGGGGCAGAACTTCTTGACATCATTCATCCCAAGGGCTATACGGAGAGACCGGTATCGGCGGCAGATAAATTGTTGGCATCATCGCCGCCGTATTTCATCGGATCTCCACCGAGCAGGGCTTCAAACCCTGTAATCCAAGACGAGCGATTTGGTAATGGTAATGGGAATTTTAGTAGTCCATTTACCATGGCATCGGTTTCGCCATCGGCTCGAGGATGTGGTGTTCCGATGGAATTCGGCAACACACAAGCTGCTGTTAGGATTGAAGGTTTTGACTGTCTTAGAAGAGATAGAAGGAGTATCTCTGCTGTAGCCTAATCCATTTTTGAAGAAAGAAAGAAGAACATAGAAAATAGTGAGAGAGAACACAGCTCAATACCAAGAATCAGCAGAGTTTTTTTTTCCTTTGGAAAGAGGATCTAATCTTAATAATTAGTAGTGTTTGATGTATATATAATTGTGATGTTTGAAGTTTGATGTTAATCAATAAAAGAGAGAACAAATCAGAGTAAACAAGGTAAGAAAAGAAGCCATTTTCTTCTGTTTTGTTATTAGGTTTGTGGAGAGTTTAAGGTATCCTTCCTATGTAATTGTAATTTGGAGGAAATTGGAATTTTGTACATCCTATTATTGTTATTAATCATAATGAGTTTTGAAGTTGTTGCT
TARGET=583,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0202
PRIMER_LEFT_PENALTY=0.019966
PRIMER_RIGHT_PENALTY=1.000269
PRIMER_LEFT_SEQUENCE=AACACACAAGCTGCTGTTAGGA
PRIMER_RIGHT_SEQUENCE=CTGCTGATTCTTGGTATTGAGC
PRIMER_LEFT=494,22
PRIMER_RIGHT=645,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=59.000
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=1.0202
PRIMER_LEFT_1_PENALTY=0.019966
PRIMER_RIGHT_1_PENALTY=1.000269
PRIMER_LEFT_1_SEQUENCE=AACACACAAGCTGCTGTTAGGA
PRIMER_RIGHT_1_SEQUENCE=GCTGATTCTTGGTATTGAGCTG
PRIMER_LEFT_1=494,22
PRIMER_RIGHT_1=643,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=59.000
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=150
PRIMER_PAIR_PENALTY_2=1.0873
PRIMER_LEFT_2_PENALTY=0.087031
PRIMER_RIGHT_2_PENALTY=1.000269
PRIMER_LEFT_2_SEQUENCE=AAGCTGCTGTTAGGATTGAAGG
PRIMER_RIGHT_2_SEQUENCE=CTGCTGATTCTTGGTATTGAGC
PRIMER_LEFT_2=501,22
PRIMER_RIGHT_2=645,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=59.000
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=1.0873
PRIMER_LEFT_3_PENALTY=0.087031
PRIMER_RIGHT_3_PENALTY=1.000269
PRIMER_LEFT_3_SEQUENCE=AAGCTGCTGTTAGGATTGAAGG
PRIMER_RIGHT_3_SEQUENCE=GCTGATTCTTGGTATTGAGCTG
PRIMER_LEFT_3=501,22
PRIMER_RIGHT_3=643,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=59.000
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=1.0956
PRIMER_LEFT_4_PENALTY=0.095324
PRIMER_RIGHT_4_PENALTY=1.000269
PRIMER_LEFT_4_SEQUENCE=AGCGATTTGGTAATGGTAATGG
PRIMER_RIGHT_4_SEQUENCE=GCTGATTCTTGGTATTGAGCTG
PRIMER_LEFT_4=396,22
PRIMER_RIGHT_4=643,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=59.000
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_04077 Ps_contig_mira-and-tgicl-ass_franssen_32053
SEQUENCE=GGGANTCAANNNAGAGGANGTCGGGGAAAGTAAAGTGAGTGAGAGAGAGAATATTAGAGATGTCAGGGAAACACGGGAGCGGAATATTAACTGTACTTATACTGCTTGCGTTGCAGTTGCAGGTACTACTGGTCGGAGGTGATTACATCCCGCCGGCCAAACCAGGCGGTTTCCTTTACAAGAATCGTCACTTCGATTACGATACCATTCGGATTGAAGCTTTCTACGATCCTTTGTGCCCTGACAGCGCTGATTCATGGCCGCCTCTTATACAAGCTCTCGATCACTACGCCGCTCGTGTTTCCCTCCTTGTTCATCTTCTTCCCTTACCTTATCATGACAATTCTTTTGTTGCTTCTCGTGCTTTGCACATAGTGAATGGTTTGAATAGATCTGCTACGTTTCCAGTGCTGCAGTCGTTCTTCAAGTATCAGGAGAAATTCTATGGTGCTCCGACACGTAACTTGTCTAGAGCAACTATTGTAGATGAAATAGTGAAGTTTGTAGCATCTGTAGTTGGGAACTCTTATCAGACTTCTATTAAGAACGGCTTCAATGACACAAACACAGATCTCCTGACCAGGGTTTCTTTTAAGTATGCTACTTCGAGAGGGGTGTCTGGAACGCCTTTTTTCTATGTAAATGGATTCGTGTTGCCGGATGCCGGAAATGCTCTTAATTACAGCGATTGGAGGAATGTTATTGACCCATTGATTGGTGCAAAGAAAAGCATCTAAAATGAAGAATCCTATCACTACATGTTTAAAGGTTTATGAAGTTCAGTTGTATTAGCACTTCATTAGTGAAAAAATAAAGAATCCAAACACTGTATCTGCAGATCAATAGCTGTGAATGTCCCAAATACGATCCATTTGAAATAAAAGAATGTCCCAAATACGAGAGATAACTTCTTCATTGCTTTCCTTCAGATTTGTAATATCTCAAATATGATATTATCCAAAATTACCATATTCTAAATATTATATATTGGTCATGATGCATACAATGT
TARGET=41,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0510
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=GTCGGGGAAAGTAAAGTGAGTG
PRIMER_RIGHT_SEQUENCE=TGGTATCGTAATCGAAGTGACG
PRIMER_LEFT=19,22
PRIMER_RIGHT=206,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.0859
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.052729
PRIMER_LEFT_1_SEQUENCE=GTCGGGGAAAGTAAAGTGAGTG
PRIMER_RIGHT_1_SEQUENCE=TCACCTCCGACCAGTAGTACCT
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=141,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.053
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=123
PRIMER_PAIR_PENALTY_2=0.0953
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.062198
PRIMER_LEFT_2_SEQUENCE=GTCGGGGAAAGTAAAGTGAGTG
PRIMER_RIGHT_2_SEQUENCE=AATCCGAATGGTATCGTAATCG
PRIMER_LEFT_2=19,22
PRIMER_RIGHT_2=214,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=60.062
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.2017
PRIMER_LEFT_3_PENALTY=0.183834
PRIMER_RIGHT_3_PENALTY=0.017846
PRIMER_LEFT_3_SEQUENCE=NGTCGGGGAAAGTAAAGTGAGT
PRIMER_RIGHT_3_SEQUENCE=TGGTATCGTAATCGAAGTGACG
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=206,22
PRIMER_LEFT_3_TM=60.184
PRIMER_RIGHT_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.2108
PRIMER_LEFT_4_PENALTY=0.183834
PRIMER_RIGHT_4_PENALTY=0.026932
PRIMER_LEFT_4_SEQUENCE=NGTCGGGGAAAGTAAAGTGAGT
PRIMER_RIGHT_4_SEQUENCE=AACTGCAACGCAAGCAGTATAA
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=117,22
PRIMER_LEFT_4_TM=60.184
PRIMER_RIGHT_4_TM=59.973
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=100
=
PRIMER_SEQUENCE_ID=SSR_04078 Ps_contig_mira-and-tgicl-ass_franssen_32054
SEQUENCE=AAAGNAAAGTGAGTGAGAGAGAGAATATTAGAGATGTCAGGGAAACACGGGAGCGGAATATTAACTGTACTTATACTGCTTGCGTTGCAGTTGCAGGTACTACTGGTCGGAGGTGATTACACTCCCCGGCCGGACCCAAACCGAGGGTCCGGTTTTCCTTTAACAAGAAYCGTCACTTCGATTACGATACCATTCGGATTGAAGTCTTTCTACGACTTCCTTTGTCGCC
TARGET=15,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04079 Ps_contig_mira-and-tgicl-ass_franssen_32055
SEQUENCE=TCAACGCAGAGTACACGGGGGCCTTGATTAAAAATAGCGTGAGTGTGTGTTTGACAACTCTGTACGTGTCTCGTTGGGCAATTCTAGTTGAAGCAATCAATGGCCACCACCAAGTCACAGTCACAGTCTAACGCCACCATTGATCCAAACTCTGGTTTCTCCTCTCATTCTAGAATCTTCCATAGCCTCCGTCCAAACGTTCCTCTTCCTCCTCCTTCTCTCCCTCTCTCCCTCACCGAGTACGTACTCTCTCTCATCCCCGCTACCGCCACCGAAAACAACATCACCGCCGTCATAGACTCCACCACCGACAATCGCCTCACTTACCCCACCTTCATCCGCCAAATCAAAGCCCTAGCTTCCTCTCTTCAATCACACACCTCTCTCTCCAAAGGCCATGTCGCATTAATCCTCACTCCTACTTCCTTACACGTCCCCGTACTCTATTTCTCCCTCCTCCTCCTTGGCGTCACCATCGCACCTGCTAACCCACTCAGTTCCCAATCCGAGTTAACTCACATCGTTCATCTCACAAAACCAGTCATCGCGTTCGCAACTTCCTCAACCGCTTCCAAAATCTCTAACCTCCCTTTCGGCACAATCATAACAGATTCTCCTTCTTTCCTTTCCATGCTTAACTCTAACTCCGAGCCTCGCCGAGTCGAAACGAGTCAATCAGACACAGCGGCGATTCTCTTCTCTTCGGGGACTACCGGACGGGTTAAAGGCGTGTTGTTAACTCATCGTAACTTCATCGCTTTGATTGGAGGGTTTTGTTCCCTGAAGCATGTTTATGATGACGGCCTTGCAAATGAACCGCATCCGGTGTCGCTGTTTCCGCTTCCACTTTTTCATGTGTTTGGATTTTTCATGATGATGAGGGCTATGTCTATGGGGGAGACTCTGGTGTTCATGGAGAGGTTTGATTTTGAAGGGATGTTGAAGATTGTAGAGAAGTATAGGGTTGTCTATATGCCAGTGTCGCCGCCGGTAGTTACGGCGTTTGCTAAGTCGGATTTGGTGAAGAAATATGATCTTAGTTCGATCCGGTTGTTAGGATCCGGTGGCGCGCCGCTTGGGAAGGAAGTTGCGGAGAGCTTCAAAGCAAAATTCCCCAATGTGGAAATTGTGCAGGGTTATGGTCTGACTGAAAGTGGAGGAGGGGCATCAAGAATGATAGGCTTTGATGAGGCTAAGCACCATGGTTCTGTGGGTCTACTAGCAGAAAATATGGAGGCTAAGATAGTTGATCCTGTTACTGGAGAGGCATTATCTCCTGGCCAAAGAGGGGAGCTTTGGATTCGAGGACCAACAATCATGAAAGGTTACATAGGAGATGACAAGGCGACTATTGAGACATTGGATTCAGATGGATGGTTGAAGACCGGCGATCTTTGTTATTTTGACTCTGATGGTTTTCTATTCATCGTGGATAGACTGAAGGAATTGATCAAATACAAAGCTTATCAGGTTCCCCCAGCTGAATTAGAGCTCATTCTTCTTACTAATCCTGAAATTGCGGATGCCGCAGTAGTTCCGTATCCTGATGAAGATGTTGGACAGATTCCAATGGCGTTTGTGGTAAGAAAGCCGGGAAGCAACATCACTGCAGCTCAGGTCATGGAGTATGTGGCAAAGCAGGTGGCACCATACAAGAAGATAAGACGTGTTTCTTTTGTTAACTCTATACCAAAATCTCCATCAGGCAAAATTTTAAGAAGAGAATTAGTGGCTATTGCTCTAAATAGTGGTTCATCCAAATTGTGATCGGGGTAAATTTTCATATATTTATGATGTCAACATATATACTGGTATTACAGTACTTTTTTCAAAGAGTAATTATTTACTACTGTTTGGAATGCATATAAAACAGTATTTAATTACTTTATGTCAGTCAACATATCAGGTTTCGAATTCGATGTATATATTCGGCGTTTGTCTTAGTAGTCTAATTTACACCTAGTGATAGAGCC
TARGET=453,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1467
PRIMER_LEFT_PENALTY=0.113182
PRIMER_RIGHT_PENALTY=0.033470
PRIMER_LEFT_SEQUENCE=AATCAAAGCCCTAGCTTCCTCT
PRIMER_RIGHT_SEQUENCE=GAATCTGTTATGATTGTGCCGA
PRIMER_LEFT=344,22
PRIMER_RIGHT=613,22
PRIMER_LEFT_TM=59.887
PRIMER_RIGHT_TM=59.967
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=270
PRIMER_PAIR_PENALTY_1=0.2245
PRIMER_LEFT_1_PENALTY=0.088501
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CTCCTCCTTCTCTCCCTCTCTC
PRIMER_RIGHT_1_SEQUENCE=GATTGGGAACTGAGTGGGTTAG
PRIMER_LEFT_1=208,22
PRIMER_RIGHT_1=505,22
PRIMER_LEFT_1_TM=60.089
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=59.091
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=0.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.2441
PRIMER_LEFT_2_PENALTY=0.113182
PRIMER_RIGHT_2_PENALTY=0.130883
PRIMER_LEFT_2_SEQUENCE=AATCAAAGCCCTAGCTTCCTCT
PRIMER_RIGHT_2_SEQUENCE=TCTGTTATGATTGTGCCGAAAG
PRIMER_LEFT_2=344,22
PRIMER_RIGHT_2=610,22
PRIMER_LEFT_2_TM=59.887
PRIMER_RIGHT_2_TM=60.131
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=0.2492
PRIMER_LEFT_3_PENALTY=0.113182
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=AATCAAAGCCCTAGCTTCCTCT
PRIMER_RIGHT_3_SEQUENCE=GATTGGGAACTGAGTGGGTTAG
PRIMER_LEFT_3=344,22
PRIMER_RIGHT_3=505,22
PRIMER_LEFT_3_TM=59.887
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=0.2517
PRIMER_LEFT_4_PENALTY=0.218249
PRIMER_RIGHT_4_PENALTY=0.033470
PRIMER_LEFT_4_SEQUENCE=AAATCAAAGCCCTAGCTTCCTC
PRIMER_RIGHT_4_SEQUENCE=GAATCTGTTATGATTGTGCCGA
PRIMER_LEFT_4=343,22
PRIMER_RIGHT_4=613,22
PRIMER_LEFT_4_TM=60.218
PRIMER_RIGHT_4_TM=59.967
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_04080 Ps_contig_mira-and-tgicl-ass_franssen_32057
SEQUENCE=TTTCCAAATATTAAGATTACAACAACCTCATTATACAATAACACTTGCTCTTCAATCTTATGTGTTCACATATTACAACTTAGATACTCATAATAATGAATCAGAAACAATAATACAAAAGTGATTAATAATAATAACAATATTAGGTAAATTAACAAGTAAACATCATATTTTTGCTTAAATCAGATTTTACTACCCAAACTTGAGTGTTTGTCCATGTAATGTAAGGAATTCTTACTAGATGAACCTTAACGTCCTTTTGGATTCATGATCTGTGAAAACACAGCACTAGGAGTTAATCCATCAGAATCTTCACTGGCCAAGCTTCTGCCACCAATGCTCATCGACATGCCACTGCTTTTGGAGTCGGTCACATTACCGTCATAACCCGGCATCGCATCCACATCCTTCTTTCCTTTTGAATCTGTAAACAATGGCTCGTCTTCACCGCATATTCCGCCAAAACCGCTGCCACTCTCCTCCGCGCTTTCTTGCAACTGCAAAGCAAATTCCAGGTTCCACAACACATCGCCCATGGATGGCCTTTCAATGCCTTGGTCGTTCACACACTTCATTGCGGTCTCGGCGAACTTCTTGAAACATTCTGGAGCTATCTTGCCTTTTAGATAAGGATCTGTTATTTGGTCAAGAATTCCTTTTTTGTAGCAATGATATGCCCACTCAGCTAGACTGACTTGTTCCTTAGCAAGTGTCGGGTTCAAAGCCGGGCGAGCACAGAGTATTTCGAATAGAACCACACCAAATGAGTATACGTCGGATTTGTCGGTGAGTTGTTGCCTCCTGAAGTATTCTGGATCCAAGTAACCAAAACTACCCTTTACCACAGTACTTACATGTGTATTATCCAATGTAGGACCTGTTTTCGACAAACCGAAATCAGAAACCTTGGCCACCCATTTCTCATCTAGTAAAATGTTTGTTGTCTTCACATCACGGTGAATGATAGTATGTTTTGCACCGGTGTGAAGATAGTGCAAACCGCGAGCAGCTCCGATGCATATCTCAAGCCTTTGCTTCCATGGAAGTGGAGGTTTCTGGGTCTTGTATAGATGCTCCCTGAGTGTTCCATAGGCCATATGATCATAGACAAGGATCATTTCGGTGTTCTCTTCACAGTATCCGATCAGTGAAACAAGGTGTCGATGACGAAGTTTGGAGAGCATTTCAATTTCAGTTTGGAATTCGTGCACACCCTGCTCGGAGAGTGGATTCCCACGTTTAATTGCAACTTTCGTTGACCCTCCATCAATTTCTCCTTTGTAAACCTTGCCAAATCCTCCCACGCCAAGAATCAAAGCCTCGTCAAAGTTGTTCGTAGCAGCCTTGATTTCAGCAAACGAGAAATGGCGACAA
TARGET=126,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04081 Ps_contig_mira-and-tgicl-ass_franssen_32082
SEQUENCE=AGGTTTNAAATNGAAAATTTGCAGATAATCATGTAACAAACCGTCTATTTTGCATTCAGGATGATCCCAATGCTTTGTTAGAAAGAAGAAAAAAAAGGAAAGGACCGACAAAAGTAGCTTCTTAAATAATATTGCAGGAAAACCCCAATTCTCTACAGACAACAACAACAACTATCTGTTCCAAAGTTTGAAATCACTAAGTACAACCATCTAAATCATGTAGGTCACTCAACATATATGTATTATAGTAATATGATGTAACACCTTTTCATCAGTCAGCCTTAGCAGGTATATGATTGCCACCAGTTGCTAGTTTGAAGAGATAAAACAGAGTCATGAGGCCGCTGAGTCCTGCAATAATACCAGCTGGCATAATCTTAGATGTTTGCAAGTATCTCTGTCCCATGATCAAAGTTAGTGCACCTGCACAAAGGATCTCAATAGCGAGAGCAAGGTAAGAGTTCTTCCGCTTTCCGAAGGCACTGAGACTGAAATAACCGGCGAGGATGAGAAGCAAACCGGTACCGACGCCTCCGGCGAGTGACGCGGTGCTTCCTTTTTGGATGAAACCGAACAATCCTCCACCTACTAATGCTAATCCGTAAGGAATCGTGAAACAGAAATCGTGCATTTTTCTTTCTCTCTGTTTTCTTCCTTCTCAAATGAACTTTGATTTTTCGGGAAAATTGGGATGAAATTGAAGTGAATCGGGAAAATAGATAGAGGAAAAAGTCTCAAATTGAAAGCTTCAGAGATTGTGTGTGCGTAAAAGCGCGTGGAAGGCACAGGT
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3895
PRIMER_LEFT_PENALTY=0.371677
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=AAAGGAAAGGACCGACAAAAGT
PRIMER_RIGHT_SEQUENCE=TGCTAAGGCTGACTGATGAAAA
PRIMER_LEFT=93,22
PRIMER_RIGHT=286,22
PRIMER_LEFT_TM=60.372
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=0.5154
PRIMER_LEFT_1_PENALTY=0.363508
PRIMER_RIGHT_1_PENALTY=0.151911
PRIMER_LEFT_1_SEQUENCE=AACCGTCTATTTTGCATTCAGG
PRIMER_RIGHT_1_SEQUENCE=CTAGCAACTGGTGGCAATCATA
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=60.364
PRIMER_RIGHT_1_TM=60.152
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.5236
PRIMER_LEFT_2_PENALTY=0.371677
PRIMER_RIGHT_2_PENALTY=0.151911
PRIMER_LEFT_2_SEQUENCE=AAAGGAAAGGACCGACAAAAGT
PRIMER_RIGHT_2_SEQUENCE=CTAGCAACTGGTGGCAATCATA
PRIMER_LEFT_2=93,22
PRIMER_RIGHT_2=312,22
PRIMER_LEFT_2_TM=60.372
PRIMER_RIGHT_2_TM=60.152
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.5380
PRIMER_LEFT_3_PENALTY=0.363508
PRIMER_RIGHT_3_PENALTY=0.174518
PRIMER_LEFT_3_SEQUENCE=AACCGTCTATTTTGCATTCAGG
PRIMER_RIGHT_3_SEQUENCE=AGGCTGACTGATGAAAAGGTGT
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=281,22
PRIMER_LEFT_3_TM=60.364
PRIMER_RIGHT_3_TM=60.175
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.5462
PRIMER_LEFT_4_PENALTY=0.371677
PRIMER_RIGHT_4_PENALTY=0.174518
PRIMER_LEFT_4_SEQUENCE=AAAGGAAAGGACCGACAAAAGT
PRIMER_RIGHT_4_SEQUENCE=AGGCTGACTGATGAAAAGGTGT
PRIMER_LEFT_4=93,22
PRIMER_RIGHT_4=281,22
PRIMER_LEFT_4_TM=60.372
PRIMER_RIGHT_4_TM=60.175
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_04082 Ps_contig_mira-and-tgicl-ass_franssen_32084
SEQUENCE=TCGTACTTGAGATTCTCTCTCGTATTCCCCTATTCGTATTTCATCATCATCATCATCTTTCACATCAGAATTCATAGGACCCTCAGAAGGATTTACTTCTCCAATTTACTTTAAAAAACTTCGACACCCTTTGAAGAATTTCTGTTGCTGGTACGGTTGATGGTGGTATCATAGTTGGGATATCCAAAGGGCTCATAGGAGAATATGGGACACCACTACCAATCTGCATCTTTCTAACCATTCCAGGAATGAGCCCAGGTGGGAACAGCGGATATGGAGGCAATTGTTCGCCAGTGCCAGATGATGTAGGGGGCAATAAATGTGCTCCAGAAGGTTGGGCGGAAGATGGTATACTCATAGAGCCTGGAAAAGCACTGGGAAGAGCAGAATTTGAAAGAAATTGCTGAGCTGCCATTGATTGCCCCAGAAGCTTATCAGGACGGTCTGGATCAAAGACAATTGAACCAGAGCTCACTCTATCAGCCTGCCACTGCTGCACAATATGGTTCGGAGTCTGCAGCATTCCGGCACCTGAATCTGCTGAAGCAGAGGATAAGTCCTTTGATGCTCCAGTAAATGAATTTGGATGTGGCCCACCAATCATCTCGCCCTTCAGTAAAGATATAGTCTCTGGATCAAATACCTCTTTAGAAGCCCAGAACTCCACCATCTGCTGCAATCTTTTCCTATATTCCTCATTGCTTTGTGGGTTA
TARGET=41,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7923
PRIMER_LEFT_PENALTY=0.758164
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=CTCTCTCGTATTCCCCTATTCG
PRIMER_RIGHT_SEQUENCE=CCTGGAATGGTTAGAAAGATGC
PRIMER_LEFT=14,22
PRIMER_RIGHT=246,22
PRIMER_LEFT_TM=59.242
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.8671
PRIMER_LEFT_1_PENALTY=0.758164
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=CTCTCTCGTATTCCCCTATTCG
PRIMER_RIGHT_1_SEQUENCE=GCAACAGAAATTCTTCAAAGGG
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=147,22
PRIMER_LEFT_1_TM=59.242
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=0.9021
PRIMER_LEFT_2_PENALTY=0.758164
PRIMER_RIGHT_2_PENALTY=0.143968
PRIMER_LEFT_2_SEQUENCE=CTCTCTCGTATTCCCCTATTCG
PRIMER_RIGHT_2_SEQUENCE=TCTTCAAAGGGTGTCGAAGTTT
PRIMER_LEFT_2=14,22
PRIMER_RIGHT_2=136,22
PRIMER_LEFT_2_TM=59.242
PRIMER_RIGHT_2_TM=60.144
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.9021
PRIMER_LEFT_3_PENALTY=0.758164
PRIMER_RIGHT_3_PENALTY=0.143968
PRIMER_LEFT_3_SEQUENCE=CTCTCTCGTATTCCCCTATTCG
PRIMER_RIGHT_3_SEQUENCE=TTCTTCAAAGGGTGTCGAAGTT
PRIMER_LEFT_3=14,22
PRIMER_RIGHT_3=137,22
PRIMER_LEFT_3_TM=59.242
PRIMER_RIGHT_3_TM=60.144
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=0.9204
PRIMER_LEFT_4_PENALTY=0.886273
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=TCTCTCGTATTCCCCTATTCGT
PRIMER_RIGHT_4_SEQUENCE=CCTGGAATGGTTAGAAAGATGC
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=59.114
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_04083 Ps_contig_mira-and-tgicl-ass_franssen_32092
SEQUENCE=TAGAAGATGGGAGAAGAGTGCTGAAAAACAAAACCAATTACCAGGGAAATTCAATATTCATCCTGTTAATCGGAAATCAACAGAACTAGGGTTTTCCTTTTCAATCGCTTTTTGATTGCGAAGAAGAAGAAGAAGAAGTAGAACAAAGATGTGGCACGAGGCGAGAAGGTCGGAGAAGAAGGTCCACGACATGATGGACGCGGCTCGAAAACGAGCACAGAGACGAGCGATTTACCTTGCTAAACGGCGTGGAGATCCCTCAACAATCAATTCAGGTCGTTGGTTCTCGTGCTCGAGCTTATCGAGATGACGCACTTTATCAAGCCACCGAGGATCAACAGGGCCTGATACCTTGGAATGGGAAACAGGATATTTTGATTGACAGATTTGATGGACGTGCTCTGCTTGATTTCATTCGGGATAGTAGTTTTAGGCGTGTCCAGGAAAAGTCGGAGGAGGAAGAAGAATTAGAAGAGTTTGTTAATTTTGAGCGTTATCGGGATTTAGTAAAGCATCGGCGTAGAGGATGTCGTTACTGATGAGGATGCTTTGCAACATGTAAATCTAGAAATGGAGGCCAAGGCTACTGCTCCATTTGCATCAGACAGATCTAATACGTCACAGCCTGCTCCAAGCAAGGGATCTTACTCACAGGTTGGGTTTTCTTATGAAGCAAATGGCAAAGAGGAATCCCATATTTCAGATGATGATGATGAAGAAGATGAAGATGACGAGGATGATGATGAGTTTAACAGTGATGATAGCAATGATGAAGGAATGGAGATAATTGCCAAAGAGTATGGGGTCAAAAGGTATGGTTGGCTTGTTTACATGGATAAGAAAGCCAAGGAGGAACAAAAAAGGCAAAAGGAGATTATCAAAGGTGATCCAGCAATTAGGAAACTGAGTCGAAAGGAAAGAAGGAAGGCTTCTCAGGTTGAGAGGGAGCGAGAGCGAGATGCTACCCGGACATCTGGAACTCGTGTGCTGCATCATGACCCCTACCGGGAATCAAGGCAAAGTCCTACTTATGAAGCTTATTCTCGCTCTAGAAGGTCAAGGTCCAGATCACGGTCCTACTCCCCATCCTATGCAAAGCGTTATTCACGGAGCAGTCATTCTGATGATATTCTTCGAAGCAAACCAAAGACTCCTAAAATAGAATACATAACTGAATTTGGGGGACCTGGAGAAGCAGATGGAACTAAGGCGGTAGGAAGGGTCCTTTTCC
TARGET=120,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1461
PRIMER_LEFT_PENALTY=0.016312
PRIMER_RIGHT_PENALTY=0.129818
PRIMER_LEFT_SEQUENCE=AGAAGATGGGAGAAGAGTGCTG
PRIMER_RIGHT_SEQUENCE=AGAACCAACGACCTGAATTGAT
PRIMER_LEFT=1,22
PRIMER_RIGHT=286,22
PRIMER_LEFT_TM=60.016
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.2033
PRIMER_LEFT_1_PENALTY=0.016312
PRIMER_RIGHT_1_PENALTY=0.186949
PRIMER_LEFT_1_SEQUENCE=AGAAGATGGGAGAAGAGTGCTG
PRIMER_RIGHT_1_SEQUENCE=CCTGAATTGATTGTTGAGGGAT
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=275,22
PRIMER_LEFT_1_TM=60.016
PRIMER_RIGHT_1_TM=60.187
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.2429
PRIMER_LEFT_2_PENALTY=0.241544
PRIMER_RIGHT_2_PENALTY=0.001315
PRIMER_LEFT_2_SEQUENCE=ATGGGAGAAGAGTGCTGAAAAA
PRIMER_RIGHT_2_SEQUENCE=ATCATGTCGTGGACCTTCTTCT
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=194,22
PRIMER_LEFT_2_TM=60.242
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.2868
PRIMER_LEFT_3_PENALTY=0.016312
PRIMER_RIGHT_3_PENALTY=0.270462
PRIMER_LEFT_3_SEQUENCE=AGAAGATGGGAGAAGAGTGCTG
PRIMER_RIGHT_3_SEQUENCE=TTTAGCAAGGTAAATCGCTCGT
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=60.016
PRIMER_RIGHT_3_TM=60.270
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.2868
PRIMER_LEFT_4_PENALTY=0.016312
PRIMER_RIGHT_4_PENALTY=0.270462
PRIMER_LEFT_4_SEQUENCE=AGAAGATGGGAGAAGAGTGCTG
PRIMER_RIGHT_4_SEQUENCE=GTTTAGCAAGGTAAATCGCTCG
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=244,22
PRIMER_LEFT_4_TM=60.016
PRIMER_RIGHT_4_TM=60.270
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=244
=
PRIMER_SEQUENCE_ID=SSR_04084 Ps_contig_mira-and-tgicl-ass_franssen_32092
SEQUENCE=TAGAAGATGGGAGAAGAGTGCTGAAAAACAAAACCAATTACCAGGGAAATTCAATATTCATCCTGTTAATCGGAAATCAACAGAACTAGGGTTTTCCTTTTCAATCGCTTTTTGATTGCGAAGAAGAAGAAGAAGAAGTAGAACAAAGATGTGGCACGAGGCGAGAAGGTCGGAGAAGAAGGTCCACGACATGATGGACGCGGCTCGAAAACGAGCACAGAGACGAGCGATTTACCTTGCTAAACGGCGTGGAGATCCCTCAACAATCAATTCAGGTCGTTGGTTCTCGTGCTCGAGCTTATCGAGATGACGCACTTTATCAAGCCACCGAGGATCAACAGGGCCTGATACCTTGGAATGGGAAACAGGATATTTTGATTGACAGATTTGATGGACGTGCTCTGCTTGATTTCATTCGGGATAGTAGTTTTAGGCGTGTCCAGGAAAAGTCGGAGGAGGAAGAAGAATTAGAAGAGTTTGTTAATTTTGAGCGTTATCGGGATTTAGTAAAGCATCGGCGTAGAGGATGTCGTTACTGATGAGGATGCTTTGCAACATGTAAATCTAGAAATGGAGGCCAAGGCTACTGCTCCATTTGCATCAGACAGATCTAATACGTCACAGCCTGCTCCAAGCAAGGGATCTTACTCACAGGTTGGGTTTTCTTATGAAGCAAATGGCAAAGAGGAATCCCATATTTCAGATGATGATGATGAAGAAGATGAAGATGACGAGGATGATGATGAGTTTAACAGTGATGATAGCAATGATGAAGGAATGGAGATAATTGCCAAAGAGTATGGGGTCAAAAGGTATGGTTGGCTTGTTTACATGGATAAGAAAGCCAAGGAGGAACAAAAAAGGCAAAAGGAGATTATCAAAGGTGATCCAGCAATTAGGAAACTGAGTCGAAAGGAAAGAAGGAAGGCTTCTCAGGTTGAGAGGGAGCGAGAGCGAGATGCTACCCGGACATCTGGAACTCGTGTGCTGCATCATGACCCCTACCGGGAATCAAGGCAAAGTCCTACTTATGAAGCTTATTCTCGCTCTAGAAGGTCAAGGTCCAGATCACGGTCCTACTCCCCATCCTATGCAAAGCGTTATTCACGGAGCAGTCATTCTGATGATATTCTTCGAAGCAAACCAAAGACTCCTAAAATAGAATACATAACTGAATTTGGGGGACCTGGAGAAGCAGATGGAACTAAGGCGGTAGGAAGGGTCCTTTTCC
TARGET=703,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1393
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.008930
PRIMER_LEFT_SEQUENCE=CCAAGCAAGGGATCTTACTCAC
PRIMER_RIGHT_SEQUENCE=TCCTTCTTTCCTTTCGACTCAG
PRIMER_LEFT=630,22
PRIMER_RIGHT=924,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=59.991
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=0.1645
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=CCAAGCAAGGGATCTTACTCAC
PRIMER_RIGHT_1_SEQUENCE=TTTCCTAATTGCTGGATCACCT
PRIMER_LEFT_1=630,22
PRIMER_RIGHT_1=902,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.1645
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=CCAAGCAAGGGATCTTACTCAC
PRIMER_RIGHT_2_SEQUENCE=TCCTAATTGCTGGATCACCTTT
PRIMER_LEFT_2=630,22
PRIMER_RIGHT_2=900,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=0.1645
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=CCAAGCAAGGGATCTTACTCAC
PRIMER_RIGHT_3_SEQUENCE=TTCCTAATTGCTGGATCACCTT
PRIMER_LEFT_3=630,22
PRIMER_RIGHT_3=901,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=272
PRIMER_PAIR_PENALTY_4=0.1645
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=CCAAGCAAGGGATCTTACTCAC
PRIMER_RIGHT_4_SEQUENCE=TCCTTGGCTTTCTTATCCATGT
PRIMER_LEFT_4=630,22
PRIMER_RIGHT_4=850,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_04085 Ps_contig_mira-and-tgicl-ass_franssen_32094
SEQUENCE=AATRTTCCACATAAAAACACANTTGCTCATAAAATTTGATTTGTCAACCCCAGAAGAAGAAAAGAGATCCAACTATCACAACAATTAACTTATAGTACATGTACATTAATTAACATAAATAACAAAATCTTGTGTTGTCTGATTTTCCTGACAAACGCAAGTAAACTAAGAATGCACATAAATATGCAGAATCTCTCACCCATCATTGGGGACTTGGGGTTTGCTCTACCATTTAGGTCCAAAAGCTTGCAAAAATGAAGCTGGCATATATACTCATTATGATGCCATTTTATTCTGGATTTGCCTATGAACATGGATAAGAAACTCTACATATGAGAAGCCACCTGCGCTCTTGTCTTCTATCATATACGAGAAGAATAACATTCCTGATGGATCGCCTTTCCTGCAGAGCTTAAGCCGGAGGTAGCAAAATCTTTGCCGCCGAATCTCATTCACCACTTCATTTAACTTCTTTGACAGTGTATTGTCAAATTGTTGCAATACAAAATGAGTAGGAATTTCATCAACTGTAGAAACACCAAACAGTTTCCGCACAATGTCTGGATTCACAGACTCTCCAGCATATATTAAACAATCATGACCATTCTCAAGAAGATATATTCCATCATCACTAATGTGTTCACTAGAAAGAGGTAGGAAGGAAGGAATAACAGATTCTTCCTCTTCCTTTGAGTCAAGGTCATGGACAGCCACCATCCTAGGATACACAAGCGGTATTGCCAATGGAGCAGATAGAGAGGACACATAATTTATCCAAAATGACCGTTCATCTATCTTTCCATCTGTTCTCAACCCTGTACTCTTAGTTAACGCAAGTGTGTACAGGGGCAAAAGCTTTAGGGCCTCCGGAAGAATAAGTTGTCCAGATGAGGACACCGTAGCGCAAAATTTACGATATGAATATAGTGCATTGGTGCAAAGGTTTGTCACTTGCTCCCGAACAAGAGGAAGTGGCTTTGAAGGGATCTCACTAGCAGCTTGCTTTAAGAAACAGCAAAATTGAGTATCCAAGTCAGCTGCACGGAATAGATTACTGAGCATACTTGTGACAGGCAGAGATAAGGTTATGACACGTATTCTTCTTTGGCCATACACAGTTGTGTATAGAAGAGCACACTGAAAGGCACATTCTGATCCATCCTGTAACTTATCATCATGTTTCAAAGTCACCATGAAGGTTTTGTCACAGTCAATCCCAGGTAAGTCAACATCTGTTGGGATACGTTTGCAAAAGTTGCCATAATATTCTTGTACTTGGATRRAAACCCTGACTGCA
TARGET=656,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04086 Ps_contig_mira-and-tgicl-ass_franssen_32106
SEQUENCE=AGTATTTTAAGCAACTCTAACCCTTTTTNCNRNGGTGGCTTGTGACAAGTTCCTCGAACACAGTCGTTTGCTAAGAGAGAGAGATCGAAAGAGAAGAAAAATGGCACTGAGAGCAACCATACTCCGCCATGTTCGCGTGCCACTCGAAGCTACTCCTAAATTGCAACCATGGAATGGTTTCTTTCGCTCCATGTCTTCACACGATGATCATATCACCAAAGAAGAAGTCGTCGATCGAGTACTCTCCGTCGTCAAAGATTTCCCCAAAGTCGATCCTTCTAAGGTGACTCCAGAAGTGCATTTTCAGAAGGATTTGGGTTTGGACAGCTTAGACACCGTGGAAATTGTAATGGCACTAGAAGAAGAGTTCAAGCTTGAAATCCCAGACCAGGAAGCTGATAAAATTGACTCTTTGCCGCTTGCTATTGAGTACATTTCTAACCACCCCATGTCTAGTTAAGTGAATCGAAGTGTTCATTTCAAATTTGGATTGCAACCTTTACCTTCCCTTTACTTCTTTCACTGGATGTTTCTAGAATTTGGTTTCTCTTGTTATCTTTGCCTTTACCCTTTCAAGTTGAAGTGAAGGTAGGTTACTGAAAATAATATGAATTGTTCTGAAAGGAGTTTGTCAGTGAAGAACAGATACTGGTTTTTTGAAATGTATTAAATTCAACTGTGGTGTTTTGGTTTATTTTTACAGTGACTGAGAAGTAGTATGAATGGAACTGTCGAGAGGCCTAAAACTCGGACAAAAATATTCGATTCTNGTTTATCACCGCCCTTTTCTCTTTGCAT
TARGET=74,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04087 Ps_contig_mira-and-tgicl-ass_franssen_32108
SEQUENCE=TTATACAATAAACAATATCCATCACATACACTTGACTAATACTGTAATTTTATTTATGAATAAAACACTAGTATAGTATAGTATCAAACTATGAATCAATATTATTACAACAACAACAAGAAAACCTGAACAAGTATTGAAACTCAAAATCAAAGACAAGTAGGACAATGAACTAACCCATTCTCATTACACTTAGGACACCTCCGCAGTGTCGAATCACTTATTTCAACCCCACTAATCACCAGCTTACAGCTTCCACCACAATCCAAACAAGGAACAAACCTAGCCCCACCACACCCCCCACAACCTTGTCTCCCAACACCTCTCTCCACACGCGTCGCATTCAATATCCTCCCAAGTCTCCCCGTTTCATTTAACTCCGTCAACTCATCCAAACCACCCAAATATCTCCCTTTCACAAACACCCGCGGCAAAACCACCCCAACCCCGGACTCTTCCTCCGTTAACAACAACTCTTTCACTTCCTTCAAAAACTCTCCGTGAAGTGCCACGTCACGCTCGTCGAATATCACCCTGTGATTCTCTAACAAGTCCCTCGCTTTGTTGCAGTTATCGAAAGTTTTTCTAACTCCTCTTAACGACGTCGTGTATATCACCACGGCGTCGTTTCCTTTCGGTGGAACTTTCTCCGGGTAGTTACTCAGTGGGTCCCTCCTTAACCGTTCGAAATTCGATTCTCTCTCTTCTTTTTGTTTCTCTAACATGTCTTTCTGTATGAATATGTTGTTCTTCAGCTTCGAAGACAACGTAGGTTTTATTGTTCTCAATCGATCACCATCCGTAGTGGATTGAGCTTGTGACTCGTTGGTGTTGTTATTCTTCGGGCTTTGTTTCGCATCTAGTTCTTCGAGTGTTCGGAAGGAAGCAACCTTGCGTGGAGGTGGTGGTTGTGACAACAGAGGAGACGGTGGTGGTGGTGTCTGAGGAGGTTTCTCTTTCTCTTGTTGGACAATTGGTTTGAGGTGTTGGAGTGTTTTGCTGACATCTTCCCATGATTGAGGAGATTTCTCGGTAGCGTCGAGGATGTGGAGAATAGGTTGAGGGAGAGCGGGTTTGTTTTCTTGTGACATGGTGTTGGTGTTGTCGTTGAGGTGTTTGAGCCATGGTTCTTCGATGGAGTTTATGTCGAACACGGCGAAGCTCGTCGGTGGTGGCTGGTATACGGCGACTGCCGTGCCGTCGATACCTTTGGAAGATGCACACCCCATTTCGTTCTTTGGATGATAGCTAGTTGCAGTGACTCTCTCCCTCCCATATACTAGTGTTGTAGTAGTAGTAC
TARGET=69,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04088 Ps_contig_mira-and-tgicl-ass_franssen_32108
SEQUENCE=TTATACAATAAACAATATCCATCACATACACTTGACTAATACTGTAATTTTATTTATGAATAAAACACTAGTATAGTATAGTATCAAACTATGAATCAATATTATTACAACAACAACAAGAAAACCTGAACAAGTATTGAAACTCAAAATCAAAGACAAGTAGGACAATGAACTAACCCATTCTCATTACACTTAGGACACCTCCGCAGTGTCGAATCACTTATTTCAACCCCACTAATCACCAGCTTACAGCTTCCACCACAATCCAAACAAGGAACAAACCTAGCCCCACCACACCCCCCACAACCTTGTCTCCCAACACCTCTCTCCACACGCGTCGCATTCAATATCCTCCCAAGTCTCCCCGTTTCATTTAACTCCGTCAACTCATCCAAACCACCCAAATATCTCCCTTTCACAAACACCCGCGGCAAAACCACCCCAACCCCGGACTCTTCCTCCGTTAACAACAACTCTTTCACTTCCTTCAAAAACTCTCCGTGAAGTGCCACGTCACGCTCGTCGAATATCACCCTGTGATTCTCTAACAAGTCCCTCGCTTTGTTGCAGTTATCGAAAGTTTTTCTAACTCCTCTTAACGACGTCGTGTATATCACCACGGCGTCGTTTCCTTTCGGTGGAACTTTCTCCGGGTAGTTACTCAGTGGGTCCCTCCTTAACCGTTCGAAATTCGATTCTCTCTCTTCTTTTTGTTTCTCTAACATGTCTTTCTGTATGAATATGTTGTTCTTCAGCTTCGAAGACAACGTAGGTTTTATTGTTCTCAATCGATCACCATCCGTAGTGGATTGAGCTTGTGACTCGTTGGTGTTGTTATTCTTCGGGCTTTGTTTCGCATCTAGTTCTTCGAGTGTTCGGAAGGAAGCAACCTTGCGTGGAGGTGGTGGTTGTGACAACAGAGGAGACGGTGGTGGTGGTGTCTGAGGAGGTTTCTCTTTCTCTTGTTGGACAATTGGTTTGAGGTGTTGGAGTGTTTTGCTGACATCTTCCCATGATTGAGGAGATTTCTCGGTAGCGTCGAGGATGTGGAGAATAGGTTGAGGGAGAGCGGGTTTGTTTTCTTGTGACATGGTGTTGGTGTTGTCGTTGAGGTGTTTGAGCCATGGTTCTTCGATGGAGTTTATGTCGAACACGGCGAAGCTCGTCGGTGGTGGCTGGTATACGGCGACTGCCGTGCCGTCGATACCTTTGGAAGATGCACACCCCATTTCGTTCTTTGGATGATAGCTAGTTGCAGTGACTCTCTCCCTCCCATATACTAGTGTTGTAGTAGTAGTAC
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04089 Ps_contig_mira-and-tgicl-ass_franssen_32108
SEQUENCE=TTATACAATAAACAATATCCATCACATACACTTGACTAATACTGTAATTTTATTTATGAATAAAACACTAGTATAGTATAGTATCAAACTATGAATCAATATTATTACAACAACAACAAGAAAACCTGAACAAGTATTGAAACTCAAAATCAAAGACAAGTAGGACAATGAACTAACCCATTCTCATTACACTTAGGACACCTCCGCAGTGTCGAATCACTTATTTCAACCCCACTAATCACCAGCTTACAGCTTCCACCACAATCCAAACAAGGAACAAACCTAGCCCCACCACACCCCCCACAACCTTGTCTCCCAACACCTCTCTCCACACGCGTCGCATTCAATATCCTCCCAAGTCTCCCCGTTTCATTTAACTCCGTCAACTCATCCAAACCACCCAAATATCTCCCTTTCACAAACACCCGCGGCAAAACCACCCCAACCCCGGACTCTTCCTCCGTTAACAACAACTCTTTCACTTCCTTCAAAAACTCTCCGTGAAGTGCCACGTCACGCTCGTCGAATATCACCCTGTGATTCTCTAACAAGTCCCTCGCTTTGTTGCAGTTATCGAAAGTTTTTCTAACTCCTCTTAACGACGTCGTGTATATCACCACGGCGTCGTTTCCTTTCGGTGGAACTTTCTCCGGGTAGTTACTCAGTGGGTCCCTCCTTAACCGTTCGAAATTCGATTCTCTCTCTTCTTTTTGTTTCTCTAACATGTCTTTCTGTATGAATATGTTGTTCTTCAGCTTCGAAGACAACGTAGGTTTTATTGTTCTCAATCGATCACCATCCGTAGTGGATTGAGCTTGTGACTCGTTGGTGTTGTTATTCTTCGGGCTTTGTTTCGCATCTAGTTCTTCGAGTGTTCGGAAGGAAGCAACCTTGCGTGGAGGTGGTGGTTGTGACAACAGAGGAGACGGTGGTGGTGGTGTCTGAGGAGGTTTCTCTTTCTCTTGTTGGACAATTGGTTTGAGGTGTTGGAGTGTTTTGCTGACATCTTCCCATGATTGAGGAGATTTCTCGGTAGCGTCGAGGATGTGGAGAATAGGTTGAGGGAGAGCGGGTTTGTTTTCTTGTGACATGGTGTTGGTGTTGTCGTTGAGGTGTTTGAGCCATGGTTCTTCGATGGAGTTTATGTCGAACACGGCGAAGCTCGTCGGTGGTGGCTGGTATACGGCGACTGCCGTGCCGTCGATACCTTTGGAAGATGCACACCCCATTTCGTTCTTTGGATGATAGCTAGTTGCAGTGACTCTCTCCCTCCCATATACTAGTGTTGTAGTAGTAGTAC
TARGET=928,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0775
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.045112
PRIMER_LEFT_SEQUENCE=TCGTTGGTGTTGTTATTCTTCG
PRIMER_RIGHT_SEQUENCE=CAAAACACTCCAACACCTCAAA
PRIMER_LEFT=822,22
PRIMER_RIGHT=998,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=60.045
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.1057
PRIMER_LEFT_1_PENALTY=0.060612
PRIMER_RIGHT_1_PENALTY=0.045112
PRIMER_LEFT_1_SEQUENCE=TTGTTCTCAATCGATCACCATC
PRIMER_RIGHT_1_SEQUENCE=CAAAACACTCCAACACCTCAAA
PRIMER_LEFT_1=778,22
PRIMER_RIGHT_1=998,22
PRIMER_LEFT_1_TM=59.939
PRIMER_RIGHT_1_TM=60.045
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.1224
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=0.096820
PRIMER_LEFT_2_SEQUENCE=ATCCGTAGTGGATTGAGCTTGT
PRIMER_RIGHT_2_SEQUENCE=GAGAAATCTCCTCAATCATGGG
PRIMER_LEFT_2=797,22
PRIMER_RIGHT_2=1030,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=59.903
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=234
PRIMER_PAIR_PENALTY_3=0.1224
PRIMER_LEFT_3_PENALTY=0.025566
PRIMER_RIGHT_3_PENALTY=0.096820
PRIMER_LEFT_3_SEQUENCE=ATCCGTAGTGGATTGAGCTTGT
PRIMER_RIGHT_3_SEQUENCE=AGAAATCTCCTCAATCATGGGA
PRIMER_LEFT_3=797,22
PRIMER_RIGHT_3=1029,22
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=59.903
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.1292
PRIMER_LEFT_4_PENALTY=0.032347
PRIMER_RIGHT_4_PENALTY=0.096820
PRIMER_LEFT_4_SEQUENCE=TCGTTGGTGTTGTTATTCTTCG
PRIMER_RIGHT_4_SEQUENCE=GAGAAATCTCCTCAATCATGGG
PRIMER_LEFT_4=822,22
PRIMER_RIGHT_4=1030,22
PRIMER_LEFT_4_TM=60.032
PRIMER_RIGHT_4_TM=59.903
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_04090 Ps_contig_mira-and-tgicl-ass_franssen_32108
SEQUENCE=TTATACAATAAACAATATCCATCACATACACTTGACTAATACTGTAATTTTATTTATGAATAAAACACTAGTATAGTATAGTATCAAACTATGAATCAATATTATTACAACAACAACAAGAAAACCTGAACAAGTATTGAAACTCAAAATCAAAGACAAGTAGGACAATGAACTAACCCATTCTCATTACACTTAGGACACCTCCGCAGTGTCGAATCACTTATTTCAACCCCACTAATCACCAGCTTACAGCTTCCACCACAATCCAAACAAGGAACAAACCTAGCCCCACCACACCCCCCACAACCTTGTCTCCCAACACCTCTCTCCACACGCGTCGCATTCAATATCCTCCCAAGTCTCCCCGTTTCATTTAACTCCGTCAACTCATCCAAACCACCCAAATATCTCCCTTTCACAAACACCCGCGGCAAAACCACCCCAACCCCGGACTCTTCCTCCGTTAACAACAACTCTTTCACTTCCTTCAAAAACTCTCCGTGAAGTGCCACGTCACGCTCGTCGAATATCACCCTGTGATTCTCTAACAAGTCCCTCGCTTTGTTGCAGTTATCGAAAGTTTTTCTAACTCCTCTTAACGACGTCGTGTATATCACCACGGCGTCGTTTCCTTTCGGTGGAACTTTCTCCGGGTAGTTACTCAGTGGGTCCCTCCTTAACCGTTCGAAATTCGATTCTCTCTCTTCTTTTTGTTTCTCTAACATGTCTTTCTGTATGAATATGTTGTTCTTCAGCTTCGAAGACAACGTAGGTTTTATTGTTCTCAATCGATCACCATCCGTAGTGGATTGAGCTTGTGACTCGTTGGTGTTGTTATTCTTCGGGCTTTGTTTCGCATCTAGTTCTTCGAGTGTTCGGAAGGAAGCAACCTTGCGTGGAGGTGGTGGTTGTGACAACAGAGGAGACGGTGGTGGTGGTGTCTGAGGAGGTTTCTCTTTCTCTTGTTGGACAATTGGTTTGAGGTGTTGGAGTGTTTTGCTGACATCTTCCCATGATTGAGGAGATTTCTCGGTAGCGTCGAGGATGTGGAGAATAGGTTGAGGGAGAGCGGGTTTGTTTTCTTGTGACATGGTGTTGGTGTTGTCGTTGAGGTGTTTGAGCCATGGTTCTTCGATGGAGTTTATGTCGAACACGGCGAAGCTCGTCGGTGGTGGCTGGTATACGGCGACTGCCGTGCCGTCGATACCTTTGGAAGATGCACACCCCATTTCGTTCTTTGGATGATAGCTAGTTGCAGTGACTCTCTCCCTCCCATATACTAGTGTTGTAGTAGTAGTAC
TARGET=1288,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04091 Ps_contig_mira-and-tgicl-ass_franssen_32109
SEQUENCE=ATTCACCTAATTTTGATATAAATAAATTGGTTCATATATTGTCAAAAAAATGAGGTACGGTGTTGAAAACAGAAAGCCTACGTGGTTAGATATAGTTGAAATAAAATGGATAGACACATTTATCCATTCCATTAATTCTCACTTTCTGACCGTCCCCGCCGGTGATGTCATCAAATCCGCGCCGGCGAATAACAACACCGTCTAAAGCAAAACCACGTTCTTCTTCCCAATACTTTATGCTACTTTTTCACTTTCACCAAACACCATTAAATCCCTTATTTAAACCTTTATTTTATTTTTTCTTCCATTTTTCACTAACCTAGATGAAATTCATGTCTCCTATGTCAACTTTTTCAATTCAAAAACCTGAATTTCGTATTTGCATTTTCAATTGCACCAAAATTACCGCTTCATGGACTATGAATAATAGGTCTATGGATTCGAGATTTTCGCCAACAGTGAATAGTAATAGTGATATGAAGAATAAGAAGAAGAAGGATGAACTCTCGGTCTCGGTGCAGCTTTCAATCTCGCCTGTTCCTAAAGTAGAAGAAAGTTTGAACTCCGACGGTCTTCGATTCGATCGGTTGCAGCCGTCGGATTATGAATTGATTCGAGAAAATAGGTTTGAGTTTGGGCAATTTGTAGCTAGAGATGCTGTGCTTGATGAAGAGTATTGGACAGCAGCCTGGCTAAGGGCAGAAAGTCATTGGGAGAATCGAACATATGAAAGATATGTTGATATCTACAAAAGGAAATTCGCCGAGCAGGAATTTAATGCGGTAAAAAGGCGGTGCAAGGCTCAAAATGGGGATAGCTGTGCTTGCATTATCGCGGTGAGGAAGGAGCAGAAGAATGTTAAGCGCTCGATAATAAAAAGTGTTGTAGGAACACTTGATTTAAATATCCGGTATTTGCTGCAAGGAGAGACTTTTCCCGGGGAACGAGTTAAGACACCTTTTTCTTGCAGCACAAACAGGACACCACCAAGTAGATACGGTTACATTGCTAACTTATGTGTTGCCAAATCGGCTCGCCGACAAGGAATCGCAAGCAACATGATGTTTTTTGCTTTTGAATCTGCAAAATCTAATGGTGTGAGGCAAGTATATGTGCATGTTGACAGAAATAACATGCCTGGACAATTATTGTACCAAAAGATGGGCTTTGAGATGGTAGAATCTGCCAATTCCCGATTGTTATTAGAGGAAACATACTTGCTACGTTTACAGATGTAAATTACAAAAGATAGTGACTTATAATTCAATTCAAAGGAGTTGCATATTTCAAAGTTCAACTGCTTCAGTTCCTCTGTAAATCAAATTCAAGGTATACCTTATAGTCTATGTAAATGTGTGTAGGTGTCCATCTTTTAATATATGGATGTCTTCCATTGTGTATTTTTTCTGGTGTCTTGTATTCTTCCTTTTATTGAATTTGATGATAAGCATATTCAAATACATTTGGATTGTAAAATGATTAATGAAACAGAACAAGAGCAAGGATATGTGTTTTTTATTTTAGTGTTCTTG
TARGET=486,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2364
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.216588
PRIMER_LEFT_SEQUENCE=CACCAAACACCATTAAATCCCT
PRIMER_RIGHT_SEQUENCE=ACTTTAGGAACAGGCGAGATTG
PRIMER_LEFT=254,22
PRIMER_RIGHT=546,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=59.783
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.4502
PRIMER_LEFT_1_PENALTY=0.363508
PRIMER_RIGHT_1_PENALTY=0.086698
PRIMER_LEFT_1_SEQUENCE=CAAAATTACCGCTTCATGGACT
PRIMER_RIGHT_1_SEQUENCE=AGGCTGCTGTCCAATACTCTTC
PRIMER_LEFT_1=397,22
PRIMER_RIGHT_1=689,22
PRIMER_LEFT_1_TM=60.364
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.5468
PRIMER_LEFT_2_PENALTY=0.363508
PRIMER_RIGHT_2_PENALTY=0.183296
PRIMER_LEFT_2_SEQUENCE=CAAAATTACCGCTTCATGGACT
PRIMER_RIGHT_2_SEQUENCE=CATCAAGCACAGCATCTCTAGC
PRIMER_LEFT_2=397,22
PRIMER_RIGHT_2=668,22
PRIMER_LEFT_2_TM=60.364
PRIMER_RIGHT_2_TM=60.183
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.5656
PRIMER_LEFT_3_PENALTY=0.491155
PRIMER_RIGHT_3_PENALTY=0.074466
PRIMER_LEFT_3_SEQUENCE=GAGATTTTCGCCAACAGTGAAT
PRIMER_RIGHT_3_SEQUENCE=TCGATTCTCCCAATGACTTTCT
PRIMER_LEFT_3=442,22
PRIMER_RIGHT_3=721,22
PRIMER_LEFT_3_TM=60.491
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.5726
PRIMER_LEFT_4_PENALTY=0.491155
PRIMER_RIGHT_4_PENALTY=0.081468
PRIMER_LEFT_4_SEQUENCE=GAGATTTTCGCCAACAGTGAAT
PRIMER_RIGHT_4_SEQUENCE=GATTCTCCCAATGACTTTCTGC
PRIMER_LEFT_4=442,22
PRIMER_RIGHT_4=719,22
PRIMER_LEFT_4_TM=60.491
PRIMER_RIGHT_4_TM=60.081
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_04092 Ps_contig_mira-and-tgicl-ass_franssen_32112
SEQUENCE=ATCCAAGAAATTNNNANANGGAGAGGTGGGGTTTGGGTTTGGGTTTTTCNAAAGGAACTTTGGCTGATGGATCTGTTGTTGCGGTTAAGCGGGTTTTAGAATCCGATTTTCAAGGGGATGTCGAGTTTTGTAATGAAGTCGAGATTATTAGTAATCTTAAGCATAGGAATTTGTTGCCTCTTAGAGGTTGTTGTGTGGTTGATGAGAGTGAGAATTATGGTGATAGAGGAAGTCAAAGATATCTTGTTTACGATTACATGCCGAATGGAAACCTCGAAGATCATCTTTTTGTGTCGAAGGATCCTTCGAAACCGAACAAATTGTTGACTTGGCCTCTAAGGAAAAACATAATCTTGGATGTAGCGAAAGGTTTGGCTTATTTGCACTACGGAGTCAAGCCTGCAATCTATCATAGGGATATCAAAGCTACTAATATTCTACTTGATGAAGATATGAGGGCAAGAGTTGCCGATTTCGGACTTGCGAAGCAAAGCAGGGAGGGTCAGTCTCATCTCACTACTAGGGTGGCGGGGACTCATGGATACCTTGCGCCGGAATACGCGCTTTATGGTCAACTGACGGAGAAGAGTGATGTGTATAGCTTTGGCGTGGTTGTTTTGGAGATAATGTGCGGGAGGAAGGCTCTTGATTTGTCTTCGTCAGGATCGCCGCGGGCGTTCTTGATTACAGATTGGGCTTGGTCGTTGGTGAAATCGGGGAAAATAGACGAGGCTTTGGATGCTTCTCTTTTGAAAGACGAAAACAATACTAGTTCGAATCCAAAGAGCATAATGGAAAGGTTTTTGCTGGTTGGAATTCTGTGTTCTCATGTAATGGTCGCTTTGAGACCGACTATTTCAGATGCTTTGAAGATGTTGGAAGGCGATATCGAAGTTCCTCAAATTCCAGACAGGCCTATGCCTCTTGGACATCCTTCATTTTATAATAACGACTGCAACGGCAACACTTTTAGCATTTCTCCGGCGTTAAGCGGCCTCAAATTGCCCACGGGAGACATGCTCAGGTAATTAATAACAAGTTAGTTAGTTAGTYANNNNAANNNCAGGATTGGAAGAAGATTGATGAACAACTTGAGAGCATGAAGATTAAGGANACAGCAGGTGGAAGGGTTACGGAAAGGTGGGGTTTTTGGTTCG
TARGET=1038,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04093 Ps_contig_mira-and-tgicl-ass_franssen_32114
SEQUENCE=AGGGACAATTCTAAACTTAAAAAAGAARACTTTTAAAACGTAATTGAGTCTAGAAAAAACCACATTTCAACGCCCGACCACAAAGCTCTCCGATCTCCATTTCCTTTTCAGATCTCGACCTTCTTCTCTTCCGCATCTTCCATTTTCCATTTTCGCTCACTTCATAGCTCATCCCACTCCACTCTCCATTTTTTCTTTTCAATAAAATCACATTATTTAATTTTCACTTAAAAAAAAAACTCATCTCAATCAATTTCTTACTCCTCTCAAGCACAGCAACCATCGTGGATCAGATCAGGTTTTTCAATTTTCGCGATGGCTGCTCCTCCTGCAAGAGCTCGATCCGATTACGATTATCTCATAAAGCTTCTTCTCATCGGTGATAGCGGTGTGGGTAAAAGTTGCCTTCTATTGCGTTTCTCAGATGGTTCATTTACAACAAGTTTTATCACAACCATTGGCATTGATTTCAAAATAAGGACAATAGAGCTTGATGGTAAGCGAATCAAATTGCAAATATGGGACACAGCTGGTCAAGAGCGGTTTCGAACCATTACAACTGCTTATTACCGTGGAGCAATGGGCATTTTGCTTGTGTATGATGTCACTGATGAGTCGTCTTTTAACAACATCAAGAATTGGATTCGCAACATTGAGCAGCATGCTTCTGACAATGTCAACAAGATATTAGTTGGGAACAAGGCTGACATGGATGAAAGCAAAAGGGCTGTTCCAACTTCGAAGGGTCAAGCTCTGGCAGATGAATATGGTATCAAGTTTTTCGAAACTAGTGCAAAAACTAATATGAATGTGGATGAGGTTTTCTTTTCAATAGCCCGGGACATCAAACAAAGACTTGCAGAAACTGACTCAAAGACTGAGCCCCAAACACTCAAGATTAACCAACCAGACCAAGGAGCCGGGTCTGCTCAAGCCACACAAAAATCTGCTTGCTGTGGTTCTTAGGGGCAGCTGTAGCAACACATTTGACCAAAGAATTTAATTTGGTCAATGACTTTCATCAGTGCTTGTATTGTTCTTTTCTTATTTTACAGTTGCTACTTATTGGGTGTGAATGGTAGATCCTTCCTATGTTGGGAAATTAATCATATATCTATTTATTGCTARTCTGTATCTGTATCTGTTTTTTCTTTCTTTCTTTTTTCAGGAACCTGACTATTGCATAATCTTTGCTAAAATCAAGAACATCGTTCGCCTACTTGGTGCTCCAATATATCTTAGTGGTACTATGAATCAAACTTTCTACAATTGATTTGATGTTTCTTTGCTTAAATTTATAAYCTAGAACAAATACTTGGTAA
TARGET=1148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04094 Ps_contig_mira-and-tgicl-ass_franssen_32118
SEQUENCE=CCGGGCTAACACTTTGATTAGTTTTAGGTGAAATAAGAAAGAAGGTTTTTGAAAAAATAATAAGAAATAGNACAGAAAGAGGTTAATATGTACAACCAATTATTACATTCGATTACCTGTCTAGCTACATATACATAATGCATTTGAAGCCACACTGTCATGCTGAAAAGAAGAAAGAGAAAGTTCCTATTATAAATTAAACCAAATAGGCTCTCATAAATTAGTGATTATTTGAGACATGTTTTAGGGGTCTCAAGCCTTAGGTAAATGAAAGTTCAAATATTCATCCATCCATTGTTCTTGTTGCGACTACTCTGGCCTCGGTCAATCCTTGTATATGTTGCCCGTCCTTTTGAACCAGCTTGTTGGTGCCGAAACTGACAATATTCAAACTCATTGACTTGTGCATCATGCAAATTGGTAACGATATTGTTGTTGTTGGAGATGGTTAAATCAGTAGAATCATCACAAATAATGGTGCTTAAACCTTTTTCCTTGGATTCTAACTGTTTGTTAACCTCCTCCACCTTGTGAACCTCTCTGCGTAATTCATCAACTCGTGTCATCAGTTTCTGCATTTGGTGCTTTCGAGCTTGTGCAAGCGTTCTCTCTAGTTGTTTTTCAATATTCTGCAAATCTTTTATGCTTAGTGTATTGAGTTCTTCTCCCAAAAAATTCCTTTGAGTCTGGGCAAGAGATTCATGCTTAACTCTTAGCATCAAAAGCTCCTCGTATAAGCCCTGTGATTCATGTTCTTCCAAGTCACCATTCTCAGACATGTTGTTGAAGCAACATTGACGATACTTCTCAATGATTTTGTTCAAGTCAGTGGAACTGTATTGAAAAAGCTTGCCAAGGCTGGAGAAGATGATAAGAGCAACTTCAGCATCACAAAGCACAGAAAGTTCGAATGCTTTCTTGAGCAAACCACTTCTTCTCTTTGAGAATGTAACTTGTCGATTGATCTTGTTCTGTATTCTCTCAAGAACAACCTTCCCTCTTCCCATCCTCTATCGCTCTTTCTCTCTCTCTCCACTCCGTACTCTGCGTTGATACACTGCTT
TARGET=430,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1978
PRIMER_LEFT_PENALTY=0.102276
PRIMER_RIGHT_PENALTY=0.095538
PRIMER_LEFT_SEQUENCE=ATCCTTGTATATGTTGCCCGTC
PRIMER_RIGHT_SEQUENCE=CGAGTTGATGAATTACGCAGAG
PRIMER_LEFT=327,22
PRIMER_RIGHT=559,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.2016
PRIMER_LEFT_1_PENALTY=0.102276
PRIMER_RIGHT_1_PENALTY=0.099308
PRIMER_LEFT_1_SEQUENCE=ATCCTTGTATATGTTGCCCGTC
PRIMER_RIGHT_1_SEQUENCE=GGTGGAGGAGGTTAACAAACAG
PRIMER_LEFT_1=327,22
PRIMER_RIGHT_1=527,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.2016
PRIMER_LEFT_2_PENALTY=0.102276
PRIMER_RIGHT_2_PENALTY=0.099308
PRIMER_LEFT_2_SEQUENCE=ATCCTTGTATATGTTGCCCGTC
PRIMER_RIGHT_2_SEQUENCE=ACAAGGTGGAGGAGGTTAACAA
PRIMER_LEFT_2=327,22
PRIMER_RIGHT_2=531,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.2780
PRIMER_LEFT_3_PENALTY=0.102276
PRIMER_RIGHT_3_PENALTY=0.175676
PRIMER_LEFT_3_SEQUENCE=ATCCTTGTATATGTTGCCCGTC
PRIMER_RIGHT_3_SEQUENCE=AAATGCAGAAACTGATGACACG
PRIMER_LEFT_3=327,22
PRIMER_RIGHT_3=579,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.176
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=253
PRIMER_PAIR_PENALTY_4=0.3229
PRIMER_LEFT_4_PENALTY=0.227356
PRIMER_RIGHT_4_PENALTY=0.095538
PRIMER_LEFT_4_SEQUENCE=CATCCATCCATTGTTCTTGTTG
PRIMER_RIGHT_4_SEQUENCE=CGAGTTGATGAATTACGCAGAG
PRIMER_LEFT_4=284,22
PRIMER_RIGHT_4=559,22
PRIMER_LEFT_4_TM=60.227
PRIMER_RIGHT_4_TM=59.904
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_04095 Ps_contig_mira-and-tgicl-ass_franssen_32118
SEQUENCE=CCGGGCTAACACTTTGATTAGTTTTAGGTGAAATAAGAAAGAAGGTTTTTGAAAAAATAATAAGAAATAGNACAGAAAGAGGTTAATATGTACAACCAATTATTACATTCGATTACCTGTCTAGCTACATATACATAATGCATTTGAAGCCACACTGTCATGCTGAAAAGAAGAAAGAGAAAGTTCCTATTATAAATTAAACCAAATAGGCTCTCATAAATTAGTGATTATTTGAGACATGTTTTAGGGGTCTCAAGCCTTAGGTAAATGAAAGTTCAAATATTCATCCATCCATTGTTCTTGTTGCGACTACTCTGGCCTCGGTCAATCCTTGTATATGTTGCCCGTCCTTTTGAACCAGCTTGTTGGTGCCGAAACTGACAATATTCAAACTCATTGACTTGTGCATCATGCAAATTGGTAACGATATTGTTGTTGTTGGAGATGGTTAAATCAGTAGAATCATCACAAATAATGGTGCTTAAACCTTTTTCCTTGGATTCTAACTGTTTGTTAACCTCCTCCACCTTGTGAACCTCTCTGCGTAATTCATCAACTCGTGTCATCAGTTTCTGCATTTGGTGCTTTCGAGCTTGTGCAAGCGTTCTCTCTAGTTGTTTTTCAATATTCTGCAAATCTTTTATGCTTAGTGTATTGAGTTCTTCTCCCAAAAAATTCCTTTGAGTCTGGGCAAGAGATTCATGCTTAACTCTTAGCATCAAAAGCTCCTCGTATAAGCCCTGTGATTCATGTTCTTCCAAGTCACCATTCTCAGACATGTTGTTGAAGCAACATTGACGATACTTCTCAATGATTTTGTTCAAGTCAGTGGAACTGTATTGAAAAAGCTTGCCAAGGCTGGAGAAGATGATAAGAGCAACTTCAGCATCACAAAGCACAGAAAGTTCGAATGCTTTCTTGAGCAAACCACTTCTTCTCTTTGAGAATGTAACTTGTCGATTGATCTTGTTCTGTATTCTCTCAAGAACAACCTTCCCTCTTCCCATCCTCTATCGCTCTTTCTCTCTCTCTCCACTCCGTACTCTGCGTTGATACACTGCTT
TARGET=1022,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2249
PRIMER_LEFT_PENALTY=0.027715
PRIMER_RIGHT_PENALTY=0.197222
PRIMER_LEFT_SEQUENCE=AAGTTCGAATGCTTTCTTGAGC
PRIMER_RIGHT_SEQUENCE=GTGTATCAACGCAGAGTACGGA
PRIMER_LEFT=902,22
PRIMER_RIGHT=1057,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=60.197
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=0.2565
PRIMER_LEFT_1_PENALTY=0.059316
PRIMER_RIGHT_1_PENALTY=0.197222
PRIMER_LEFT_1_SEQUENCE=GAGCAACTTCAGCATCACAAAG
PRIMER_RIGHT_1_SEQUENCE=GTGTATCAACGCAGAGTACGGA
PRIMER_LEFT_1=874,22
PRIMER_RIGHT_1=1057,22
PRIMER_LEFT_1_TM=60.059
PRIMER_RIGHT_1_TM=60.197
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.2565
PRIMER_LEFT_2_PENALTY=0.059316
PRIMER_RIGHT_2_PENALTY=0.197222
PRIMER_LEFT_2_SEQUENCE=AGAGCAACTTCAGCATCACAAA
PRIMER_RIGHT_2_SEQUENCE=GTGTATCAACGCAGAGTACGGA
PRIMER_LEFT_2=873,22
PRIMER_RIGHT_2=1057,22
PRIMER_LEFT_2_TM=60.059
PRIMER_RIGHT_2_TM=60.197
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.2565
PRIMER_LEFT_3_PENALTY=0.059316
PRIMER_RIGHT_3_PENALTY=0.197222
PRIMER_LEFT_3_SEQUENCE=AAGAGCAACTTCAGCATCACAA
PRIMER_RIGHT_3_SEQUENCE=GTGTATCAACGCAGAGTACGGA
PRIMER_LEFT_3=872,22
PRIMER_RIGHT_3=1057,22
PRIMER_LEFT_3_TM=60.059
PRIMER_RIGHT_3_TM=60.197
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=0.2775
PRIMER_LEFT_4_PENALTY=0.080323
PRIMER_RIGHT_4_PENALTY=0.197222
PRIMER_LEFT_4_SEQUENCE=CAAAGCACAGAAAGTTCGAATG
PRIMER_RIGHT_4_SEQUENCE=GTGTATCAACGCAGAGTACGGA
PRIMER_LEFT_4=891,22
PRIMER_RIGHT_4=1057,22
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=60.197
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_04096 Ps_contig_mira-and-tgicl-ass_franssen_32119
SEQUENCE=TTTTAGTTTAGGAAATTAATCAGAGTTGAAACAAATTTAAATTAACTTAAATTTATTTCTTTTCTTTACCTATAAATTGTGTACTAAACTGAAAACCAAATTARRCCTTARGAAGAAAGAAATATGCGAATGTGGCAGTAAATAGATACACAGAAGATAACAAACAAGGAATGAAGCCCGAGACATAAAGACTAGTGATGGAAACCAACACATGAATCCTCAAACATTTCATGTCATTGACGATAATGTTTCTTACTTCTTCTCTGTTAAAACATTGTGCTAACTACACAATCTGCAAAACCATAGGTGAAAATGAATCCGATTTCATTGCAAGAATGAATAGAGGGACCTTATTAAATCTAATCATCGCGGAAAGTCTTTTGACCAGATCCTCTAGGCCTCCCCTTAAACATTTTCCTGTTCTTTTTCTTTTTTCCTTTCTTAGCTTCTTCTTTCACAAACTTCTCTTTCTCTCTTTTTGTCAAGAAATCTGTTACTACTAAATAGATAACCCCGATACTGATAACGGATAAGCCAATGAAAGCGAGGAAGAAAAGAACTGATACGATTACGGACCCGGCATCAACGGGGGTTGTGACCAATTGCTCTGCGGCATCAACGGCGGCGTCTTCGGCAGCTGCAGCAGCAAGAGGAATTGGCAAAAGTTTGGACTTATTATGCAAAAGGATGGATTTCGTGGATGGGGAGATAATGGGAGAGAAAGATGAAGTAGTTGGAAGAGAGCCAAAGCGTGTGTGGAAATGGAAAGAAGAAGGAGATTTTGAAAGGAAGGTTGCAATTTGTGGAGATTGTGCAAATGCAGCACTGGCCATGGTCATGGCTTCTTACCCTCACTCACTCAGAGCAATGGCAATCACTTGATTCACTTTTTTGCTTATGTGACGTGGCCCAACCACGGTTTTGGTTGGGGTTGCTTTGCTATCTTTTTGATTAGATCCTATCAGTGAATCCTATTACTACTAGAGTAACTTCTCCC
TARGET=851,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04097 Ps_contig_mira-and-tgicl-ass_franssen_32120
SEQUENCE=GTCGGGGGAATTATGAGAAAAGCGGTGTTATTTGAAGACAGAGAGAAAATAATAAAAAAAGAATAACACAGTATACAAAAGCGTAGAAGTAGCAAGTGTGAACCGACAGTAGATTACAGTATGAGTTGGGTTTTGCCATTCTCAATCTCAGTGCTATTATGGATATGTATCTTCTCTTCTTCTTCTTCTTCATCTTCAGTAGCTGCTGTTACAGTGAGTGGTGGATGCAATAAACCTGCGATTCTATTCGTGTTCGGCGATTCCAACTCGGACACGGGTGGACTCGTTTCTGGACTCGGTTTCCCTGTCAATCTTCCCAATGGTCGCACTTTCTTTCACAGATCCACCGGTCGTTTGGCCGATGGACGATTAGTCATCGATTTTCTCTGCCAGAGTTTGAATACAAATTTTTTGACCCCATACTTGGACTCCTTGTCCGGATCAACATTCACAAATGGAGCAAACTTTGCCGTGGTGGGATCCTCTACCCTCCCAAAATATGTTCCTTTCGCTTTAAATATACAGGTCATGCAGTTCCAGCATTTCAAAGCTCGTAGCCTTCAACTTGCTACCTCAGGTACAAAAAGTATGATCAACGAAGAAGGCTTCCGCAATGCACTTTATTTGATTGATATTGGGCAAAATGACCTAGCTGATTCATTTACCAAAAAAAATCTATCATATGTGCAAGTCATCAAGAAGATCCCAACAGTAATTGCTGAAATTGAGAATGCTGTTAAGAGTTTGTATAATGAAGGTGGCAGGAAATTTTGGCTTCACAATACCGGACCCTTTGGCTGTCTTCCTAAATTTATCGCACTGTCTGACAAGAAAGACCTCGATTCATTTGGATGTATTTCTAATTTCAATTCTGCTGCAAGATTGTTCAACGAAGCGCTGTATCATTCCAGTCAAAAACTAAGAACTGAGTTGAAGGATGCTGCCTTAGTCTATGTTGATATTTATGCCATTAAGAATGATCTCATTGCAAATGGCACCAAATATGGTTTCTTGAATCCATTGATGGTATGCTGCGGCTATGGAGGACCACCTTATAATTTCGACGTAAGAGTGACATGTGGTCAACCTGGTTATCAGGTTTGCGATGAAGGATCTCGGCATGTGAGTTGGGATGGAATCCATTACACCGAGGCTGCGAATTCATGGATAGCTTCGAAAATACTGTCGGCGGCGTATTCCACGCCACGGTTACCTTTTGGTTTCTTCTGCAGCCACTGAAATCTGATGGTTGATTATAGTATGCACATTACAAGGGTGCAAAACAGTGAACATACGTTTAGTTCATTTGCTGTTGTATTACTACTATATGTGTAGTTCTCCAAGAACATTGTTTTGTCATGCATCCCATGTCTCCTTTCACTTTTAGCCATGAACAAATAATAGTATAATTATATCTCGTTAAATCAATTCAATTGATAATTATTGGTTGCAATGTTNCNAAAAGGGGCATTTCACTTGTT
TARGET=175,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1435
PRIMER_LEFT_PENALTY=0.115769
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=GTATGAGTTGGGTTTTGCCATT
PRIMER_RIGHT_SEQUENCE=ACACGAATAGAATCGCAGGTTT
PRIMER_LEFT=118,22
PRIMER_RIGHT=252,22
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=0.1435
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.027715
PRIMER_LEFT_1_SEQUENCE=GTATGAGTTGGGTTTTGCCATT
PRIMER_RIGHT_1_SEQUENCE=AACACGAATAGAATCGCAGGTT
PRIMER_LEFT_1=118,22
PRIMER_RIGHT_1=253,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.1860
PRIMER_LEFT_2_PENALTY=0.158317
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=AAGTAGCAAGTGTGAACCGACA
PRIMER_RIGHT_2_SEQUENCE=ACACGAATAGAATCGCAGGTTT
PRIMER_LEFT_2=86,22
PRIMER_RIGHT_2=252,22
PRIMER_LEFT_2_TM=59.842
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.1860
PRIMER_LEFT_3_PENALTY=0.158317
PRIMER_RIGHT_3_PENALTY=0.027715
PRIMER_LEFT_3_SEQUENCE=AAGTAGCAAGTGTGAACCGACA
PRIMER_RIGHT_3_SEQUENCE=AACACGAATAGAATCGCAGGTT
PRIMER_LEFT_3=86,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=59.842
PRIMER_RIGHT_3_TM=60.028
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=0.2598
PRIMER_LEFT_4_PENALTY=0.232060
PRIMER_RIGHT_4_PENALTY=0.027715
PRIMER_LEFT_4_SEQUENCE=AGTATGAGTTGGGTTTTGCCAT
PRIMER_RIGHT_4_SEQUENCE=ACACGAATAGAATCGCAGGTTT
PRIMER_LEFT_4=117,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=136
=
PRIMER_SEQUENCE_ID=SSR_04098 Ps_contig_mira-and-tgicl-ass_franssen_32125
SEQUENCE=GAAACAAACAAATGGTGCCACGCGTCAATTGCGAAACCCCATATAACAATTGCCTCTAACTATTGGTCTAGCGATCTTAAACTAAATAATCGTAAATTCGTAATCAATCAATCGAGATTGAGGATGGGTGTGGTGATAATAGACGGTACCACCGTAACAGACTTCATAGCCGACGACGAGCGTTTCACAGACAGCGTCAACGAACAGTTTTCATCTCTCGATCTCAACAACGACGGCGTTTTATCACGCGCCGAGCTTCGCACAGCCTTCGAATCGATGAGACTGATCGAGGCACACTTCGGCATCGACGTATCTACTCCGCCGGAGCAACTCACGCGCCTTTACGACTCCGTGTTCGACAGTTTTGACGGTGACGGAAGTGGCACTGTCGATCGTGACGAGTTTAAATCCGAGATGAAGAAGATCATGCTCGCGATCGCTGATGGATTGGGATCTTCGCCTATTCAGATGGTTCTGGAAGATGATTCGCAGAGTCTTCTTCAGAAAGCGGCGGATCTTGAAGCTTCTAAGTACGCTGCTGCCGTTTGATTGCTTGTCGTTTTGGTTGTTTTTGTGTCCTGTTATAGGTCGTTTTGGTTATCGTTTTGTATATTTACGGATATTGTTAGAATTTTGATGTCAGTGTGATTAAAATCTGTGTTTGATTTGCACTGCATAAGTTTTCTTTTGCTTTTCATCCTGGTCTACTCCAATAAATCTTCCGACTTCAATTTTCTTTATTGTACTTGTGCAAGTCATAAATAGTTTAAAATAGTTTATGGTACTTTTTACTAACNCTTCGCAATGTTCTTTCATGTTTCAAATATGTCAACCTAGAAAAAACT
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4330
PRIMER_LEFT_PENALTY=0.428626
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=CAATTGCGAAACCCCATATAAC
PRIMER_RIGHT_SEQUENCE=ACCGTCTATTATCACCACACCC
PRIMER_LEFT=25,22
PRIMER_RIGHT=146,22
PRIMER_LEFT_TM=60.429
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=122
PRIMER_PAIR_PENALTY_1=0.5380
PRIMER_LEFT_1_PENALTY=0.533658
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=GAAACCCCATATAACAATTGCC
PRIMER_RIGHT_1_SEQUENCE=ACCGTCTATTATCACCACACCC
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=146,22
PRIMER_LEFT_1_TM=59.466
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=0.6292
PRIMER_LEFT_2_PENALTY=0.533658
PRIMER_RIGHT_2_PENALTY=0.095538
PRIMER_LEFT_2_SEQUENCE=GAAACCCCATATAACAATTGCC
PRIMER_RIGHT_2_SEQUENCE=TCGTCGGCTATGAAGTCTGTTA
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=175,22
PRIMER_LEFT_2_TM=59.466
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=0.7720
PRIMER_LEFT_3_PENALTY=0.428626
PRIMER_RIGHT_3_PENALTY=0.343387
PRIMER_LEFT_3_SEQUENCE=CAATTGCGAAACCCCATATAAC
PRIMER_RIGHT_3_SEQUENCE=GTCTGTTACGGTGGTACCGTCT
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=161,22
PRIMER_LEFT_3_TM=60.429
PRIMER_RIGHT_3_TM=60.343
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=137
PRIMER_PAIR_PENALTY_4=0.8256
PRIMER_LEFT_4_PENALTY=0.428626
PRIMER_RIGHT_4_PENALTY=0.396944
PRIMER_LEFT_4_SEQUENCE=CAATTGCGAAACCCCATATAAC
PRIMER_RIGHT_4_SEQUENCE=CGTTGTTGAGATCGAGAGATGA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=231,22
PRIMER_LEFT_4_TM=60.429
PRIMER_RIGHT_4_TM=60.397
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_04099 Ps_contig_mira-and-tgicl-ass_franssen_32128
SEQUENCE=CTTCACAAATGAAACAAACTAGTCTCATTTCTATCACCTTTAAATTTAGTTAAATAGTTTGTCCATCCATTTCGTTATTGTGTGTTCCTATGATTTATAGCCAATAACTACAATAAAAAAAATTCTTAATACACATTGTCACGCCAAGAACATCGGCTAAATTCTCTTACCTTAATGATAACAAAAACAAACCGTCACACCAAGAACATCTACAAAATACTCTTTTCTTAATGACAACAAAAACAAATCCCCTCAGAATAGAAACCCCCTCAAAGCCAGTGTGTCCAACATGATAAAACTAAATATGCAATTTAACTCGCTCGCATAGAAGAATGTGGGGATGAATCAGACTTTTCAACGGCTAGATAATGATCAAGCATATCATCTTCGTCATCTTCGAAATCCTCATCATCATCAAAGTCAATGTCTCCCTCATCATCCTCATCCAATTCCTCAATAACTACAGCAGCCCCTTTCATCCTTCCACTTTCAAACCACTGTCTACCACTGAGTTTCTTTTCCTTTGGAGTAGCAAGCACGGACTCTGGCATTAGCTTGGCTCGTTCAAGTGCTAGTTCAGCCTCAAACCTTTCCCTCCATGCCAAAAATGTTTCAACAGTAACTGGTTCTCCATGGGGTATCACTATATCTTCTTTGGCGGCCTCCTCTGCTTCAGCAATCCCGCCATCAGGGTCCTCACCAAACCTCTCAGCCAACCACTCTTTAGCTGAGGAAACGAGTGTGTAGATCATAGCCATGCCAAGATTTTCAGATGCCTCTTGTTGAAGTTTTTCTTTCAAAATCCTTAAATCTTCAGACGGTATTCCTTGCAAACTGTTCACATTTAAAAGTGGAGGTTCGTCAGGATACTTTTCTGTATGAGCGAAGATCAAAGCCAGTTGAGCTGGATTAGTAATTGACCCGTCCTCCTCTTCCTCCTGTGCAGTGACCTTAATCTGGAAGCACCTATTGGAAGTGCTTAAACCACTTTCACCAGAGTGTATTTCTTTGAATTCATCCATAAGTATAGCTTCTAATGCTTCAATCTCCATTTCTTGTTCCTGCACGTAGTCTGTCATGATAACTTGGTTGGATTGGATTGACGAAAATGGGTGTGAAGCGAAGAAAGTATAGGTTGAAGAAGAAGACCTAATTCACTCAGACACGCCCCCTTCCCAATTCTCTCTATTTTCTTCCATCCATGGACCCCCGACGAAC
TARGET=406,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1778
PRIMER_LEFT_PENALTY=0.028588
PRIMER_RIGHT_PENALTY=0.149184
PRIMER_LEFT_SEQUENCE=AGAATAGAAACCCCCTCAAAGC
PRIMER_RIGHT_SEQUENCE=ATGAAAGGGGCTGCTGTAGTTA
PRIMER_LEFT=254,22
PRIMER_RIGHT=478,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=60.149
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.1778
PRIMER_LEFT_1_PENALTY=0.028588
PRIMER_RIGHT_1_PENALTY=0.149184
PRIMER_LEFT_1_SEQUENCE=AGAATAGAAACCCCCTCAAAGC
PRIMER_RIGHT_1_SEQUENCE=AAGGGGCTGCTGTAGTTATTGA
PRIMER_LEFT_1=254,22
PRIMER_RIGHT_1=474,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.1778
PRIMER_LEFT_2_PENALTY=0.028588
PRIMER_RIGHT_2_PENALTY=0.149184
PRIMER_LEFT_2_SEQUENCE=AGAATAGAAACCCCCTCAAAGC
PRIMER_RIGHT_2_SEQUENCE=TGAAAGGGGCTGCTGTAGTTAT
PRIMER_LEFT_2=254,22
PRIMER_RIGHT_2=477,22
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.1809
PRIMER_LEFT_3_PENALTY=0.028588
PRIMER_RIGHT_3_PENALTY=0.152322
PRIMER_LEFT_3_SEQUENCE=AGAATAGAAACCCCCTCAAAGC
PRIMER_RIGHT_3_SEQUENCE=ATTGAGGAATTGGATGAGGATG
PRIMER_LEFT_3=254,22
PRIMER_RIGHT_3=457,22
PRIMER_LEFT_3_TM=59.971
PRIMER_RIGHT_3_TM=60.152
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.2104
PRIMER_LEFT_4_PENALTY=0.061184
PRIMER_RIGHT_4_PENALTY=0.149184
PRIMER_LEFT_4_SEQUENCE=TCAGAATAGAAACCCCCTCAAA
PRIMER_RIGHT_4_SEQUENCE=AAGGGGCTGCTGTAGTTATTGA
PRIMER_LEFT_4=252,22
PRIMER_RIGHT_4=474,22
PRIMER_LEFT_4_TM=59.939
PRIMER_RIGHT_4_TM=60.149
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_04100 Ps_contig_mira-and-tgicl-ass_franssen_32129
SEQUENCE=AATAATAAATAATAATAATAAAGTCATAGAGATTCAGAGTGGTGTTGAAACCCAAAGAGATTCAGAGATTCATCAATGGCGGAAGGAGATGATAATGTGATCGAACACAAAGCATATGCACGAGTTGGTTTTCTCGGAAATCCAAGCGATGTCTATTACGGCAAAACAATCGCTTTCAGTCTTTCCAATTTCTATGCCACCGTTCACCTTCGTCCTTCCGATGAACTCGTCATTCAGCCACATCCTACTCACGATCTTGTCAACTTCGATTCTCATCATCAATTGGTTGATAGATTGAACAGTGAGGGTTATTATGGTGGAGTTCGATTGCTTATGGCTATTTATAAAGTTTTTTATAATTATTGTAAAGAGAATGAGATTCATCTTCCTCATACCAATTTCACTCTCTCATATGATACTAATATCCCTCGTCAGAGTGGACTTTCAGGTTCTAGTGGGATTGTGTGTGCTGCCTTGAACTGCTTTCTTGATTTCTACAAAGTCAGGCATCTAATCAAGGTGGAGGTGAGGCCTAACCTTATCCTTGCTGCTGAGGAAGAACTTGGAATAGTTGCTGGTCTTCAAGATCGAGTTGCACAAGTTTATGGTGGCCTAGTTTACATGGACTTTAACAAGGAAAACATGGACAAATTAGGGCACGGAATTTATATACCAATGGATTTGAGTCTACTCCCACCTCTATACCTCATCTATGTAGCGAATCCTAGCGACTCTGGGAAGGTTCATAGTAAAGTACGTCAGAGATGGCTTGATGGTGATGAGTTCATCGTGTCAACTATGCTTGAAGTGGCAAATATTGCGGAAGAAGGAAAGACAGCACTAGAAGAGAAGGACTATTCCAAACTAGCATCTCTCATGAATAAAAATTTCGACCTGCGAAGGTTGATGTTCGGAGATGATGCACTTGGTGATGTGAACATAAAAATGGTAGAGATTGCTAGAAAGGTTGGAGCGGCGTCGAAATTTACAGGTAGCGGGGGAGCTGTTGTTGCATATTGTCCTGAAGGAACTTCTCAAGTAAAGCTTCTTGAGGATGAATGTCATGAAGCAGGATTTGTTTTAATACCTCTTGAACCTTTTCCTTCTCGGTTAAATGAAATAGACTTGAAAACCCTGCAGTTGTAATGTACTATTCTTATTCTGACGATTTGAAAATTAGTTGTTATGAGTTAGAATTTTCAATATGAGATGCTCTTTTTATTTATGAATTACAAAAATAAAAACAATGGGTTANAAAATTGTATTTTTAGTTGGGTTTAAAGTACTTAGATTGTCTC
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04101 Ps_contig_mira-and-tgicl-ass_franssen_32139
SEQUENCE=CAAAATANNNTCAACNAAAAGAAACATGTATTATCTCAAGACTATAGTAATCTAAATATAATGACAATTGAAGCTAACAAGCAACAAAGTAGAAAAATCTTCAACATCCCAAAATTCTTCATCATAACAAACAAAATTCAATACATAAAACTCAATTTCATTAAAATTAAACAATCTAACAGTAGTCTATTACTTGAACGTATCATCAGGAACCATCCTAAGCTGTTTCCTCTGCTTCGACTTCGCAATCCTCTTCAAAGTCTTAGGGTTCGTGATCTTCTGAAGCTTAGTACCAGTTCTAAGAATATTCTCTTTCTTCTTCTTCTCCCTCTCTTCTTTCTTCTTCCTCTTCTCAACCTTATTCAACCTTATCTCTTCTTTCAACTCCGTCATCCTCTCCTTAAACGCCGTTTTAATACTCTTCGCTCTCGCCCTCTCCTCAAACGTCGTTCCCTTCTTGCTAACCTGCATCGCCGACGACCGTCTCTGCCGCGCCTGCTTCCAGTTCCTGCCGGAGACTCTCCCAGCAATCACTCCGTCGTAGGTAGGTTTACCAAACGAAAAAGACGAAAACGACGGTTTGTTGGGATCTGATTCCGATGGAAGCGCGGATCTCTTCGCTGGAGGAGGGGCGGTTTCGTCGGTCTCCATCTCCATGGCATGGATTTCTTCCATCCTTGCATCCTCGATTGCTTGAGCTTGTTCACGTTTGCGCTTGTAGGTTTTGCCGCCGAAGCCTTCATCGAGATTGCGAAAATCGATAGTTGAAGCCATGGTTGGTATCAGAGGAGTGTGAAGTGATACCCCCCGACTCTGCGTTGATACACTGCTT
TARGET=314,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0913
PRIMER_LEFT_PENALTY=0.081468
PRIMER_RIGHT_PENALTY=0.009871
PRIMER_LEFT_SEQUENCE=AACGTATCATCAGGAACCATCC
PRIMER_RIGHT_SEQUENCE=GGCGAGAGCGAAGAGTATTAAA
PRIMER_LEFT=196,22
PRIMER_RIGHT=432,22
PRIMER_LEFT_TM=60.081
PRIMER_RIGHT_TM=60.010
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.2643
PRIMER_LEFT_1_PENALTY=0.254467
PRIMER_RIGHT_1_PENALTY=0.009871
PRIMER_LEFT_1_SEQUENCE=CCATCCTAAGCTGTTTCCTCTG
PRIMER_RIGHT_1_SEQUENCE=GGCGAGAGCGAAGAGTATTAAA
PRIMER_LEFT_1=212,22
PRIMER_RIGHT_1=432,22
PRIMER_LEFT_1_TM=60.254
PRIMER_RIGHT_1_TM=60.010
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.3282
PRIMER_LEFT_2_PENALTY=0.081468
PRIMER_RIGHT_2_PENALTY=0.246726
PRIMER_LEFT_2_SEQUENCE=AACGTATCATCAGGAACCATCC
PRIMER_RIGHT_2_SEQUENCE=AGAGGATGACGGAGTTGAAAGA
PRIMER_LEFT_2=196,22
PRIMER_RIGHT_2=397,22
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.247
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.3282
PRIMER_LEFT_3_PENALTY=0.081468
PRIMER_RIGHT_3_PENALTY=0.246726
PRIMER_LEFT_3_SEQUENCE=AACGTATCATCAGGAACCATCC
PRIMER_RIGHT_3_SEQUENCE=GAGAGGATGACGGAGTTGAAAG
PRIMER_LEFT_3=196,22
PRIMER_RIGHT_3=398,22
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=60.247
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.3303
PRIMER_LEFT_4_PENALTY=0.081468
PRIMER_RIGHT_4_PENALTY=0.248820
PRIMER_LEFT_4_SEQUENCE=AACGTATCATCAGGAACCATCC
PRIMER_RIGHT_4_SEQUENCE=AGGATGACGGAGTTGAAAGAAG
PRIMER_LEFT_4=196,22
PRIMER_RIGHT_4=395,22
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_04102 Ps_contig_mira-and-tgicl-ass_franssen_32141
SEQUENCE=CGGGGTATATATTCTTGTTTACTNATTGAGACTTTCAAACACAGAAACTGAAAAGTAGCGAAGATGTCGCATTTGCATTTACAGCTACACTCACAAGCACCGTCGCTCTCTTCTTCTTCAACTTCTCGTTTTTCTTTCCTTAAGCTTTTCACAAACAACACTTTCTCTTTATCTCCAACAACAAACTCTCTTCCTTTCAAACCCCTAACTATCAGATGCCGCCACTCTGACGTCTTTGAAACTGGAACCAACCCTTCTATTCCCACTTCAACCCCAACAACAACAACCCTTCAGTTGGAGCTTTACCACCTAGGGTTTATGTTGGTCATTCCATTTACAAGGGGAAGGCTGCTCTCACTATAACTCCTCGACCGCCGGAGTTCATGCCTCTGGATTCAGGGGCATATAAGATATCTAAGGATGGTTATTGTGCTGCTTCAGTTTGCCCCATCGGTTGGTCCGCGCCAGTATGATTGGAATAGAAARNANGTTTTCTCGTTATCGGTGGATGAAATGGGAAGTGTGATTAGCCTTGGTGCAAGGGACCGTTGTGAATTTTTTCATGATCCTTACAAGGGAAAAAGTGATGAAGGCAAAGTCAAAAAAAGTTTTGAAAGTTGAGCCTTTTCCGGATGGTTCTGGATTCTTCTTTAATCTCGGTGTTCAAAACAACCTTGCGAACTTGGAAGAAAGCATCACTCTCCCCGTAACAAAATCAGAGTTATCAGTTTTGAGCGCAATTTTCAAGTTCATCATGCCGTACCTACTTGGTTGGCATACCTTTGCAGACTCGATAAATCCGGAATATTCTGTGGCGGCAGCGAGTGTGGCACGCAATGCCAACCCCAAATATGGAGGAGACTATGAATGGAGCAGATAGGGATATGCTGTTTTGCTTGTATTACACAACCCTCTTCACACTATGAACTTCTCAACTTTTGAAATGTCTTAAATGGTTTTTACCTGTCAGATTAGTTGTCTAAGGGGAATTGCCATGGTAACTTTTTGTTGAAATAGATGAGTAGGATTCTTGGTGCTATTAATTTTTTTAGAAAACCAATCAGAATCGTTNATAAAAGTTCATCAAAATCGAATCAAACCATTTCATTTTATCTTGAATCRAATTGAACTAAATTGTTAGTTCACTAAGTTCAGTAC
TARGET=275,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04103 Ps_contig_mira-and-tgicl-ass_franssen_32145
SEQUENCE=ATCGAGACCAAACGCTTTCTTTATTCCGTTCAAACGCTATTCGTCTTTTTTATTCCGTTTTTTTTGTTTCTATTTAAACCTCCTTCTTCTCCCTCATTTCTCCATTATCTATCTGTTCTCTGCATTTTTCTCGCTTCCGTAACTTCCCTTTAAGCAATATAGTCCAAATGGAGAACGGGCACAACYTCCAGGAAGTTTCAAACCCGAAATATGATTGCCTATTGTTTGATATTGATGACACACTTTACCCTTTGAGTTCTGGAATGTCAGCGCATACAGCCAGAAATATTGAAGAGTATATGCTTCAAAACCTTGGAATGGAGGCTGAGAAAGTTCCTGAGTTGTGCAATTCATTATACAAGACTTATGGGACAACGATGGCTGGTCTTAGGGCAATTGGTTATGACTTTGACTATAATGACTTTCATAGTTCTGTTCATGGGAGATTGCCTTATAGTCTATTGAAACCGGACCCAGTTCTCAAGGGGATTTTGCAAAGCTTGCCTTGTAGGAAACTGGGTTTTACAAATGCGGACACGGGGCATGCGATAAGAGTGCTTAAAAGACTTGGGTTGGAGGACTGTTTTGAAAGAATTATAAGCTTTGATACTCTGAATTCCTTGGACGATATCAGACCTCCTGATGACAAAAATGGCGAAGAAATTTTTTTGATTTTTTGCGAGTACACRCGCCGGCCTGATTCTGGTATGGTGCTTCCAAAGACACCGGTTATCTGCAAACCCTTTGATAATGCATTTGAAAAGGCTTTCAAGTTGGCTGACATTGACCCTCAAAGAACATTGTTCTTCGATGATAGTATCCGCAATTTAATGACAGCGAAACGTTTGGGCCTCCATACGGTTGCGGTGGGTACATCGGTGCGTAGTACAGGAGTGGATCATGCATTAGAGAGTATCCATAATATCAGAGAGGCATTTCCAGAGCTATGGGAAGCCGATGAGAAGAACGAGATTGTTAACTACAAAGTTGCAATTGAAACAACAGTAAAAGCATAGCTTATATTTGTTTGTTTGTGGTGTCTTAAGTATAGTATAGTGGCCATGGCACCTAAGTAATTGTTATTGTTTTTTTTGTATGATTGATCTTGTATTACGCTTAAACCCTTTGCATCTATAAAGTGTCAATTGAAACACAGATCTATAATGTCAATTATCTTATGCTCAACTTTTGGTCTTTGTTTAATG
TARGET=1023,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04104 Ps_contig_mira-and-tgicl-ass_franssen_32151
SEQUENCE=GGAATTTTATTTATCATAACGCGTAGAGTGAGACCAGAGTGTACAATAATAAGACCAAAAAAACAGAAACAAAATAGAAAACATAGTAACAAAACANAACAAANNAAAACACCAAAACCACTTCCAATGGCTTCTTCTAGGARGAGTTTTCTTTCAAGATCAAGTTACATTTTTCCAACACCCTCAGAGATAAACACAAAATCATCCGAAGCAACTGAGTTCGAGCTCGATGAAGCTGATGTATGGAACATGCCATGTTCAAATTCCGACAACATGACAGAATCAAAAAAGTGTGTGCTACCAGGTTTGAAGAGAGTTTCTAGAAAAATGGAATCCAATAACCGAGTTAACCCTATAGGTTCATCTTCTTTACCGATGAATATACCAGATTGGTCAAAGATTTTGAAAGATGAATACAAGAAGAAGAAGAAGAACAAAGAGAGTAGTGATGATGATGAAGATGAAGATGGCTATGGTGGAGAAATTCGTTTACCTCCTCATGAGTATCTTGCTAGAACTAGAGGAGCTTCTCTTTCAGTTCATGAAGGGAAAGGAAGGACTTTGAAAGGTAGGGATTTGCGTAGTGTTAGGAATGCTATTTGGAAGAAAGTTGGTTTTGAAGATTAATATATATTTCAACTGATACAAGTCGGTTGAACATCATTATCAGAATTCGAACTCTGTATTCTCACATCTGTGTGCGTGAGTTTGTAGTGATTATTATCTTTCGTCTATCTATAAAAGATGATCTTCCTTTCATTTTGAGTGTCTAAGTGTTTTTATTAACTTTTAGGATTTGGATCACTCATCATGCTTGTATATTGTAATTAACTATATTAATCTTTTTATGTTTAATCTTTTGTGGCCAYNGTAAAA
TARGET=418,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04105 Ps_contig_mira-and-tgicl-ass_franssen_32151
SEQUENCE=GGAATTTTATTTATCATAACGCGTAGAGTGAGACCAGAGTGTACAATAATAAGACCAAAAAAACAGAAACAAAATAGAAAACATAGTAACAAAACANAACAAANNAAAACACCAAAACCACTTCCAATGGCTTCTTCTAGGARGAGTTTTCTTTCAAGATCAAGTTACATTTTTCCAACACCCTCAGAGATAAACACAAAATCATCCGAAGCAACTGAGTTCGAGCTCGATGAAGCTGATGTATGGAACATGCCATGTTCAAATTCCGACAACATGACAGAATCAAAAAAGTGTGTGCTACCAGGTTTGAAGAGAGTTTCTAGAAAAATGGAATCCAATAACCGAGTTAACCCTATAGGTTCATCTTCTTTACCGATGAATATACCAGATTGGTCAAAGATTTTGAAAGATGAATACAAGAAGAAGAAGAAGAACAAAGAGAGTAGTGATGATGATGAAGATGAAGATGGCTATGGTGGAGAAATTCGTTTACCTCCTCATGAGTATCTTGCTAGAACTAGAGGAGCTTCTCTTTCAGTTCATGAAGGGAAAGGAAGGACTTTGAAAGGTAGGGATTTGCGTAGTGTTAGGAATGCTATTTGGAAGAAAGTTGGTTTTGAAGATTAATATATATTTCAACTGATACAAGTCGGTTGAACATCATTATCAGAATTCGAACTCTGTATTCTCACATCTGTGTGCGTGAGTTTGTAGTGATTATTATCTTTCGTCTATCTATAAAAGATGATCTTCCTTTCATTTTGAGTGTCTAAGTGTTTTTATTAACTTTTAGGATTTGGATCACTCATCATGCTTGTATATTGTAATTAACTATATTAATCTTTTTATGTTTAATCTTTTGTGGCCAYNGTAAAA
TARGET=447,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04106 Ps_contig_mira-and-tgicl-ass_franssen_32154
SEQUENCE=CTTACTGCTCAGGTTTTCAACAATGGTTCTGTACCAGCCTCAGCCCAAGCTACCTTCCCATACATATTTGCAGTCAGTAAATTCTTGCAGCCTGGAAGTTTTGATCTTGTTGGCACTATTATCTACGAGATAGACGAGCATCCATACCAAAGCACCTTCTTTAATGGCACTATTGAAGTTGTTGAATCGGGTGGGTTTCTTAGCATCGAATCTGTTTTTCTTGTTACTCTTGGAGCTTCCCTCCTTGTCCTCTTGGGGCTATGGATTCAAGGTCAAGTACAAAACCTATCTAAGAAATCAAAGAGAGCTCCCAAAGTTGAAGTTGGAACTAGGTCTATAGATGCTTCGACGGACGAATGGCTACAGGGAACTGCATATACTCAGTCTCTTTCCAGCAAATCAAAGAAGAAGAAGTAGATGAATAGTTTTCCTTTTCCTCCAGTTCTTCAGCCAAACATGACTAGAGATGTAACACCATGTTAATTAGTTTGAGAGCGCGAGGAATTGTGGCTGAATGATGACGACCTGCCTTTTCTGTTGTAGCTTTAAAGAAATTTTACTTTTTTCCCTTCCATTGAGTTTGAGCTTTTTATAAGAAAAAACTAGCAGGAATGAGTTAGGAAGGATAGAACTTTTGAGAAGTGGCACAATTTTTGTTACATAATGTTCATTTTAC
TARGET=403,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0590
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=0.052069
PRIMER_LEFT_SEQUENCE=TCAGTCTCTTTCCAGCAAATCA
PRIMER_RIGHT_SEQUENCE=AAGCTACAACAGAAAAGGCAGG
PRIMER_LEFT=380,22
PRIMER_RIGHT=545,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.1301
PRIMER_LEFT_1_PENALTY=0.078029
PRIMER_RIGHT_1_PENALTY=0.052069
PRIMER_LEFT_1_SEQUENCE=AGAGCTCCCAAAGTTGAAGTTG
PRIMER_RIGHT_1_SEQUENCE=AAGCTACAACAGAAAAGGCAGG
PRIMER_LEFT_1=303,22
PRIMER_RIGHT_1=545,22
PRIMER_LEFT_1_TM=59.922
PRIMER_RIGHT_1_TM=59.948
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.1699
PRIMER_LEFT_2_PENALTY=0.027712
PRIMER_RIGHT_2_PENALTY=0.142170
PRIMER_LEFT_2_SEQUENCE=GTGGGTTTCTTAGCATCGAATC
PRIMER_RIGHT_2_SEQUENCE=CTGAAGAACTGGAGGAAAAGGA
PRIMER_LEFT_2=190,22
PRIMER_RIGHT_2=449,22
PRIMER_LEFT_2_TM=59.972
PRIMER_RIGHT_2_TM=59.858
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.2167
PRIMER_LEFT_3_PENALTY=0.164639
PRIMER_RIGHT_3_PENALTY=0.052069
PRIMER_LEFT_3_SEQUENCE=GAAATCAAAGAGAGCTCCCAAA
PRIMER_RIGHT_3_SEQUENCE=AAGCTACAACAGAAAAGGCAGG
PRIMER_LEFT_3=293,22
PRIMER_RIGHT_3=545,22
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=253
PRIMER_PAIR_PENALTY_4=0.2202
PRIMER_LEFT_4_PENALTY=0.078029
PRIMER_RIGHT_4_PENALTY=0.142170
PRIMER_LEFT_4_SEQUENCE=AGAGCTCCCAAAGTTGAAGTTG
PRIMER_RIGHT_4_SEQUENCE=CTGAAGAACTGGAGGAAAAGGA
PRIMER_LEFT_4=303,22
PRIMER_RIGHT_4=449,22
PRIMER_LEFT_4_TM=59.922
PRIMER_RIGHT_4_TM=59.858
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_04107 Ps_contig_mira-and-tgicl-ass_franssen_32155
SEQUENCE=ACCCCAATACAAATCTGGTTTATTTCAGCGTTGTCGGGGACTCACGTTTCATTTGGAAAATCGCAACACACCACCATGGCCTCCTTCAACACCATTTGGATTTTCTCTCTCGCTCTTCTCCTCATCGCTTCTCCTTTCCTCCAAGTTGTTAGGTGTAAGTCAGAATCAAGTGAAGCTGTGGAGACTACTGAAAGTGATATTGGCATTGTTGGTGATGATGCTCAAGACTTTGATGGAGTTGGAACCTTTTCTGCTGCTCCGGGGATTGATACTATAAGTGTGTTTCCTAAAAATATTGCAAAATTGGTTACAGCTGGAGAAGACACCGAGCTGCTAGTTGGTGTTAAAAATGATGGCGAGTCTGGCTTGAATGTTGTTGCGATCAAGGCCAGTATTCACCTTCCTGTCGATCATCGTCTGTATGTTCAAAATCTTACTGCTCAGGTTTTCAACAATGGTTCTGTACCAGCCTCAGCCCAAGCTACTTTCCCATACATATTTGCAGTCAGTAAATTCTTGCAGCCTGGAAGTTTTGATCTTGTTGGCACTATTATCTACGAGATAGACGAGCATCCATACCAAAGCACCTTCTTTAATGGCACTATTGAAGTTGTTGAATCTGGTGGGTTTCTTAGCATCGAATCTGTTTTTCTTGTTACTCTTGGAGCTTCCCTCCTTGTCCTCTTGGGGTTATGGATTCAAGGTCAAGTACAAAACCTATCTAAGAAATCAAAGAGAGCTCCCAAAGTTGAAGTTGGAACTAGGTCTATAGATGCTTCGACAGACGAATGGCTACAGGGAACTGCATATACTCAGTCTCTTTCCAGCAAATCAAAGAAGAAGAAGTAGATGCATAGTTTTCCTTTTCCTCCAGTTCTTCAGCCAAACATGACTAGAGATGTAACACCATGTTAATTAGTTTGAGAGCGCGAGGAATTGTGGCTGAATGATGACGACCTGCCTTTTCTGTTGTAGCTTTAAAGAAATTTTACCTTTTTCCCTTCCATTGAGTTTGAGCTTTTTATAAGAAAAAACTAGCAGGAATATGAGTTAGGAAGGATAGAACTTTTGAGAAGTGGCACAATTTTTGTTACATAATGTTCATTTTACTATTTATTTTGTTTTGTTTTTGTTCTATCCGAGGATTCCGAAAGGGGTCAGGCTCAAGTAAAATAACCATAATGATCAAAGGGGGYCAAAGGCCCTAATTTTYCAAGTTGGATTTCAAAATGTTCAGATAT
TARGET=835,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04108 Ps_contig_mira-and-tgicl-ass_franssen_32159
SEQUENCE=GTCGGGGATGTTCAAAATGTTGGAAAACTATATGTTACTACNAGATTAGATTAAATATTAGAATTAGTGGCTTATCAAAAATAAAAAAGAATTATTAAATAAAAACCACTTGATAATGTATTACTAGATCTCTTTATAGTTCTACTCATTTGACTCCAGTGATAACTGTGAAGAATTCAACGTTGAAAGTTGAAATATGGCAATAAATTTTGCATCTTGGATCATCCTTCTTCATCTTCTCCTAATCCTATTGTGTTCTAATGGAAACCAAGCAAGGGAAATTGTTGAAACAGAAAACAAATCATTGGAAATTCAACACAACCATCATCATCATCATCATCATCAACATAATCATATGACTCATAATATTGATCCTTCATTGATGGTCTTTTTCACTTTGAAAGATTTGAAAGTTGGAAAAACAATGCAAATTTATTTCCCTAAGAGAGACCCTTCAACTTCGCCAAAGTTATGGCCAAAAGAAAAAGCTGAATCACTTCCTTTCTCATCAAACCAACTCTCATATCTTCTCAAATTCTTCTCTTTTTCTCCAAACACTCCACAAGCCATGGCTATGGAAAACACCTTACAAGAATGTGAAAGCAAACACATCAAAGGAGAAGTCAAATTCTGTGCTACTTCGTTACAATCTATGCTGGAATTCACACAAAAAACTCTTGGTTCAACTTCTGAAATCCAAGTTTATGCAACTTTGCATAAAACAAAGTCAAGTGTCACTTTTCAAAATTACACTATAGTAGAAATTATGATGGAAATTCTAGCTCCAAAAATGGTGGCTTGTCACACTGTGCCTTACCCTTTTGCTGTTTTCTATTGTCATAGTCAAGAAAGTGAGAATAGGGTGTATAAAGTATTACTAGGGGGTGAGAATGGAGATAAAGTTGAAGCTATGGTTGTTTGTCATATGGATACATCACAATGGTCACCTAGTCATGTTTCATTTCAAGTTCTTGGTGTTACACCAGGAAGTTCTTCTGTGTGTCACTTTTTTCCAGCTGATAATTATATTTGGGTCCCTAAATTGAAATCACAAGGTTCATCTAGCATGTGATTTGTAATAATTTAGTACTAGTGTGCTTGCAATTTGTGTTTATGTTGCTAAAAATAATGTGTTGTATTCAAAGCTAAATAAGTTTTTTATGAGTATATGTTAAAGTCTCTACTTTTATAATTTTACTTTTAATTTTGAATCATGATGATCTTGATCTGATTATTCAATATCTCTATCAAAAACTATAGTTGTTAAATGAGTTTATATANTAATAAAAATGTATTTATTTAGTTTGGCCTCATGGTCTTCACCTCAACATCTATTAGACTCCAATTGTAATATAGTAAATTTTTAATATATACCTTATTATTTTTACG
TARGET=323,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7016
PRIMER_LEFT_PENALTY=0.045749
PRIMER_RIGHT_PENALTY=0.655875
PRIMER_LEFT_SEQUENCE=GCATCTTGGATCATCCTTCTTC
PRIMER_RIGHT_SEQUENCE=TTGAAGGGTCTCTCTTAGGGAA
PRIMER_LEFT=211,22
PRIMER_RIGHT=457,22
PRIMER_LEFT_TM=60.046
PRIMER_RIGHT_TM=59.344
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=247
PRIMER_PAIR_PENALTY_1=1.0546
PRIMER_LEFT_1_PENALTY=0.045749
PRIMER_RIGHT_1_PENALTY=1.008837
PRIMER_LEFT_1_SEQUENCE=GCATCTTGGATCATCCTTCTTC
PRIMER_RIGHT_1_SEQUENCE=GCGAAGTTGAAGGGTCTCTCT
PRIMER_LEFT_1=211,22
PRIMER_RIGHT_1=463,21
PRIMER_LEFT_1_TM=60.046
PRIMER_RIGHT_1_TM=60.009
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=1.1611
PRIMER_LEFT_2_PENALTY=0.045749
PRIMER_RIGHT_2_PENALTY=1.115306
PRIMER_LEFT_2_SEQUENCE=GCATCTTGGATCATCCTTCTTC
PRIMER_RIGHT_2_SEQUENCE=GTTGAAGGGTCTCTCTTAGGGAA
PRIMER_LEFT_2=211,22
PRIMER_RIGHT_2=458,23
PRIMER_LEFT_2_TM=60.046
PRIMER_RIGHT_2_TM=60.115
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=1.2476
PRIMER_LEFT_3_PENALTY=0.462203
PRIMER_RIGHT_3_PENALTY=0.785407
PRIMER_LEFT_3_SEQUENCE=TTCTAATGGAAACCAAGCAAGG
PRIMER_RIGHT_3_SEQUENCE=TGGTTTGATGAGAAAGGAAGTG
PRIMER_LEFT_3=255,22
PRIMER_RIGHT_3=515,22
PRIMER_LEFT_3_TM=60.462
PRIMER_RIGHT_3_TM=59.215
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=1.3020
PRIMER_LEFT_4_PENALTY=0.045749
PRIMER_RIGHT_4_PENALTY=1.256249
PRIMER_LEFT_4_SEQUENCE=GCATCTTGGATCATCCTTCTTC
PRIMER_RIGHT_4_SEQUENCE=GCGAAGTTGAAGGGTCTCTCTT
PRIMER_LEFT_4=211,22
PRIMER_RIGHT_4=463,22
PRIMER_LEFT_4_TM=60.046
PRIMER_RIGHT_4_TM=61.256
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_04109 Ps_contig_mira-and-tgicl-ass_franssen_32160
SEQUENCE=TTAAGTCATATAAAAGAACAAAAAAGAAAAGAAAAGAAAATAGGATCATGATGATGAAGAAGAGGAAATCGATAAAAGAAAGAAANATCGTGTAATTAATTTACGTAATTTAACCAACGAGCACCAAGGCAGTGTTCGAGACTGAATTTCAGAGAGAGTTTGATCAATTTTCATGTACAAGGACAAGGCCTCTTTGGAAAAGTGGATCTGAAAAACGCATCCATGTCTTCATTACTCGAAACCTCGAAGAATTGAAATTCATCCATAAGCTTCCTCTTACAAGAAACGAACGGCTTCGGTTTCTTCGGCGCCGGCGGACAATTCAAGATCTCCGGTATCTTGCTTTCCTTCGAAGTCGGAGTCCGGTAACTCTCATCGTTTTCGTTCTCGTTCTCGTGCTCTCCGTCAACGACGGCGCCGTCGTTTTCGTTTTCGGTTTCAGGTTGTGCGATTGGAGACGACGGGATATCGTCTACCGGAACCTCGGGGGTTTGATTGGAGGAAACAACATCGGCGTCAACGTCAACGTCGTCGAGTTTGACGGCGGAGATTCGGAGTTTAGGCAATTGATGGTGGAGGTGGAGATCGGTGGACATGATCGGTAAAGGCGTGTTTGGAAGGAGAGAAAATTACTTAACGTGATGTCCCCGCGTACTTCTTTGCGTTGATACCAACTGCTT
TARGET=22,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04110 Ps_contig_mira-and-tgicl-ass_franssen_32161
SEQUENCE=AAGCAGTGGTATCAAACGACAAGAGTACNCGGGGGCTTTCCGTAAAGTTTTTTCCTCACATTCCAACTTATCTATATATATTCACTAACAAACGCAGTTTCACGCACATCACGTTAAGTAATTTTCTCTCCTTCCAAACACGCCTTTACCGAATCTCCGCCGTCAAACTCGACGACGTTGACGTTGACGCCGATGTTGTTTCCTCCAATCAAACCCCCGAGGTTCCGGTAGACGATATCCCGTCGTCTCCAATCGCACAACCTGAAACCGAAAACGAAAACGACGGCGCCGTCGTTGACGGAGAGCACGAGAACGAGAACGAAAACGATGAGAGTTACCGGACTCCGACTTCGAAGGAAAGCAAGATACCGGAGATCTTGAATTGTCCGCCGGCGCCGAAGAAACCGAAGCCGTTCGTTTCTTGTAAGAGGAAGCTTATGGATGAATTTCAATTTCGAACCAAATCCAATCACAACAACAACGCTCATAACTGCTTTTTACTCCAAACGAGTTAACACCGAGTCCACTCACTCACTCAACCATGAGCCGATTCAGAAGATACGAAATCATCGAAGAAGAACCATTACCTTTTCCCTTACCCTTCTGTCACTCATCTTCTTCACATTTCTTCGTAACAGAAACCCTACCATTTGCTTCCTTTGTTGAAGAATCTCTCCCGTTCAATCTCGATCTATGTCTTCTCCCTAGTCATTTCCACGCGGATCTGGTCCGCGTGGATCGTACTGCGTCGTTTTTGCAGAAACGAGTTGAGAAGCAACAGAAACTCGGTCTGGAGAGTTTGAGTGACCGAGTCGCCGAGTTGGAGTCGCGATTCGAGCAGATCCTCGGCGGAAAGAAGAAGGAGGAGAAGAAGAAGAAGAACATTGACGGTGGAGGTGGATGTAATCGGAAGTACACTTAAGAAAAAATAGGAAGCTATATGTCTCCATTTGGTTTGGCCGTGTAATTTTGTCTTATTATCTATTTGGTTCCTATATTTA
TARGET=525,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3203
PRIMER_LEFT_PENALTY=0.319597
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=TACCGGAGATCTTGAATTGTCC
PRIMER_RIGHT_SEQUENCE=GAAGCAAATGGTAGGGTTTCTG
PRIMER_LEFT=366,22
PRIMER_RIGHT=656,22
PRIMER_LEFT_TM=60.320
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.5436
PRIMER_LEFT_1_PENALTY=0.319597
PRIMER_RIGHT_1_PENALTY=0.224014
PRIMER_LEFT_1_SEQUENCE=TACCGGAGATCTTGAATTGTCC
PRIMER_RIGHT_1_SEQUENCE=TTTCGTATCTTCTGAATCGGCT
PRIMER_LEFT_1=366,22
PRIMER_RIGHT_1=565,22
PRIMER_LEFT_1_TM=60.320
PRIMER_RIGHT_1_TM=60.224
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.5500
PRIMER_LEFT_2_PENALTY=0.319597
PRIMER_RIGHT_2_PENALTY=0.230415
PRIMER_LEFT_2_SEQUENCE=TACCGGAGATCTTGAATTGTCC
PRIMER_RIGHT_2_SEQUENCE=AAAGGAAGCAAATGGTAGGGTT
PRIMER_LEFT_2=366,22
PRIMER_RIGHT_2=660,22
PRIMER_LEFT_2_TM=60.320
PRIMER_RIGHT_2_TM=60.230
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.5500
PRIMER_LEFT_3_PENALTY=0.319597
PRIMER_RIGHT_3_PENALTY=0.230415
PRIMER_LEFT_3_SEQUENCE=TACCGGAGATCTTGAATTGTCC
PRIMER_RIGHT_3_SEQUENCE=AAGGAAGCAAATGGTAGGGTTT
PRIMER_LEFT_3=366,22
PRIMER_RIGHT_3=659,22
PRIMER_LEFT_3_TM=60.320
PRIMER_RIGHT_3_TM=60.230
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=0.6057
PRIMER_LEFT_4_PENALTY=0.531262
PRIMER_RIGHT_4_PENALTY=0.074466
PRIMER_LEFT_4_SEQUENCE=CAACAACGCTCATAACTGCTTT
PRIMER_RIGHT_4_SEQUENCE=ATTGAACGGGAGAGATTCTTCA
PRIMER_LEFT_4=475,22
PRIMER_RIGHT_4=684,22
PRIMER_LEFT_4_TM=59.469
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_04111 Ps_contig_mira-and-tgicl-ass_franssen_32161
SEQUENCE=AAGCAGTGGTATCAAACGACAAGAGTACNCGGGGGCTTTCCGTAAAGTTTTTTCCTCACATTCCAACTTATCTATATATATTCACTAACAAACGCAGTTTCACGCACATCACGTTAAGTAATTTTCTCTCCTTCCAAACACGCCTTTACCGAATCTCCGCCGTCAAACTCGACGACGTTGACGTTGACGCCGATGTTGTTTCCTCCAATCAAACCCCCGAGGTTCCGGTAGACGATATCCCGTCGTCTCCAATCGCACAACCTGAAACCGAAAACGAAAACGACGGCGCCGTCGTTGACGGAGAGCACGAGAACGAGAACGAAAACGATGAGAGTTACCGGACTCCGACTTCGAAGGAAAGCAAGATACCGGAGATCTTGAATTGTCCGCCGGCGCCGAAGAAACCGAAGCCGTTCGTTTCTTGTAAGAGGAAGCTTATGGATGAATTTCAATTTCGAACCAAATCCAATCACAACAACAACGCTCATAACTGCTTTTTACTCCAAACGAGTTAACACCGAGTCCACTCACTCACTCAACCATGAGCCGATTCAGAAGATACGAAATCATCGAAGAAGAACCATTACCTTTTCCCTTACCCTTCTGTCACTCATCTTCTTCACATTTCTTCGTAACAGAAACCCTACCATTTGCTTCCTTTGTTGAAGAATCTCTCCCGTTCAATCTCGATCTATGTCTTCTCCCTAGTCATTTCCACGCGGATCTGGTCCGCGTGGATCGTACTGCGTCGTTTTTGCAGAAACGAGTTGAGAAGCAACAGAAACTCGGTCTGGAGAGTTTGAGTGACCGAGTCGCCGAGTTGGAGTCGCGATTCGAGCAGATCCTCGGCGGAAAGAAGAAGGAGGAGAAGAAGAAGAAGAACATTGACGGTGGAGGTGGATGTAATCGGAAGTACACTTAAGAAAAAATAGGAAGCTATATGTCTCCATTTGGTTTGGCCGTGTAATTTTGTCTTATTATCTATTTGGTTCCTATATTTA
TARGET=867,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3601
PRIMER_LEFT_PENALTY=0.083883
PRIMER_RIGHT_PENALTY=0.276218
PRIMER_LEFT_SEQUENCE=AACAGAAACTCGGTCTGGAGAG
PRIMER_RIGHT_SEQUENCE=AGACAAAATTACACGGCCAAAC
PRIMER_LEFT=776,22
PRIMER_RIGHT=974,22
PRIMER_LEFT_TM=59.916
PRIMER_RIGHT_TM=60.276
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.3601
PRIMER_LEFT_1_PENALTY=0.083883
PRIMER_RIGHT_1_PENALTY=0.276218
PRIMER_LEFT_1_SEQUENCE=CAGAAACTCGGTCTGGAGAGTT
PRIMER_RIGHT_1_SEQUENCE=AGACAAAATTACACGGCCAAAC
PRIMER_LEFT_1=778,22
PRIMER_RIGHT_1=974,22
PRIMER_LEFT_1_TM=59.916
PRIMER_RIGHT_1_TM=60.276
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=0.3995
PRIMER_LEFT_2_PENALTY=0.123278
PRIMER_RIGHT_2_PENALTY=0.276218
PRIMER_LEFT_2_SEQUENCE=CTTCTCCCTAGTCATTTCCACG
PRIMER_RIGHT_2_SEQUENCE=AGACAAAATTACACGGCCAAAC
PRIMER_LEFT_2=697,22
PRIMER_RIGHT_2=974,22
PRIMER_LEFT_2_TM=60.123
PRIMER_RIGHT_2_TM=60.276
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.4316
PRIMER_LEFT_3_PENALTY=0.155362
PRIMER_RIGHT_3_PENALTY=0.276218
PRIMER_LEFT_3_SEQUENCE=GGTCTGGAGAGTTTGAGTGACC
PRIMER_RIGHT_3_SEQUENCE=AGACAAAATTACACGGCCAAAC
PRIMER_LEFT_3=787,22
PRIMER_RIGHT_3=974,22
PRIMER_LEFT_3_TM=60.155
PRIMER_RIGHT_3_TM=60.276
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=0.6051
PRIMER_LEFT_4_PENALTY=0.328883
PRIMER_RIGHT_4_PENALTY=0.276218
PRIMER_LEFT_4_SEQUENCE=TTTGCAGAAACGAGTTGAGAAG
PRIMER_RIGHT_4_SEQUENCE=AGACAAAATTACACGGCCAAAC
PRIMER_LEFT_4=753,22
PRIMER_RIGHT_4=974,22
PRIMER_LEFT_4_TM=59.671
PRIMER_RIGHT_4_TM=60.276
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_04112 Ps_contig_mira-and-tgicl-ass_franssen_32165
SEQUENCE=GAAGGACAGNGGTAGTCAACGCACGAGGTAACGCCGGGGGAAGGGTACGACAACCCCTCCTAACTAACCCCCTTTTCCTTAACCGAAATACCAGCTAATAAATAGAAAAGACGAAAACAAAAACAACACGCCGCNACTCTTATTTTCTTCTTCTCTTCCTCTTCTCTTAATCTAATCTAGAAAACAAAAATGAAGTCGGTAGAACTCAAGCGGTTATGCTTTCTCTCTTTATAACAATCGTAATATCAGAGAGGGTAACAGAGAGAGAAAGAAAGAATGGCTGTGGAATATCATTGTTGCGAACCGGGATTCTTCATCCACATACTCATAATCGTACTGTTGGTGGTGTTCGCCGGATTAATGTCCGGCCTCACCTTGGGACTCATGTCTTTAAGTCTCGTTGATCTTGAAGTTCTAGCTAAATCCGGTACTCCTCGCGATCGTAAACATGCCGAGAAAATATTGCCTGTTGTGAGAAATCAGCATTTGTTGCTTTGTACTTTGCTGATTTGCAATGCTGCCGCCATGGAGGCACTTCCAATTTTTCTTGATAGTCTTGTTACTGCTTGGGGTGCTATCCTTATTTCGGTCACGTTGATTCTTCTCTTTGGTGAGATTATACCCCAATCAATTTGTTCTCGATATGGTTTAGCTATTGGTGCATCAGTGACTCCCTTTGTTCGTGTGCTTGTATGGATATGTTACCCAGTTTCTTTTCCAATTAGCAAGTTGTTGGACTATTTGCTGGGTCATCGACACGAAGCTCTTTTCCGCAGAGCTGAGTTGAAAACGCTCGTAGATTTGCATGGTAATGAGGCTGGAAAAGGTGGGGAACTTACTCATGATGAAACAACAATAATTGCTGGGGCGCTTGAACTTAGTGAGAAAACAGCCAGTGATGCCATGACTCCTATATCTGAAATATTTGCCATTGATATTAATTCGAAGCTTGATAGGGGGCTGATGAACGAAATATTGGGCAAAGGGCATAGCAGAGTCCCAGTCTATTATGAGCAGTCCACAAACATTATTGGACTTATACTGATCAAGAATTTATTGACAATTCATCCGGAAGATGAAGAACCTGTGAAGAATGTAACGATACGCAGGATTCCAAGGGTTCCAGAAAGTATACCACTTTATGACATTTTGAATGAGTTTCAGAAGGGCCATAGTCACATGGCCGTTGTTGTAAGACAGTGTGACAAGAAGAAGCAACCATCTTCCCAAAATAATGCAAATGATTCGGTGAGAGAAGTGAAGGTGGATATTGATGGTGAAAAGCCTCCCCAAGAGAAAGTCTTGAAACCCAAGATACCAATCCAAAAGTGGAAAAGCTTTCCACATTCAAACAGGTCAAATAGGGGCAATTCTCGGGGCAGAAAATGGACAAAAAATATGTACTCAGATATTTTAGAGATAGATGGTAGTCCCCTTCCAAATATCCCTGAAGAAGAAGAAGCCATTGGAATAATTACAATGGAAGATGTCATTGAAGAACTTTTACAGGAGGAGATCTTTGATGAGACGGATCATCATTTTGAAGACTCATGACAATGGCTTTTCCATTAAACCTGATGTTTTCACATTCTTGTTTTGGACCCTGTAGATAATTAATTAGATAATGAGGATGAGTGTGAGAGCTTTTGTTTAGGGATCTTAAATGTTATTGTAAAGAACATATCAGTAATGAAATGTAGGCACAAGAAAGCAGTTATTACTTGTACGTCAAGCTTTCTTTATGGAGGTGTACATTAATGGATCTTTAAGGCAATGAAGCATGCCAGCAAATCGTGGTGTATGCCATTAAAGAAAA
TARGET=266,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2493
PRIMER_LEFT_PENALTY=0.041553
PRIMER_RIGHT_PENALTY=0.207756
PRIMER_LEFT_SEQUENCE=CAAGCGGTTATGCTTTCTCTCT
PRIMER_RIGHT_SEQUENCE=AGTATGTGGATGAAGAATCCCG
PRIMER_LEFT=206,22
PRIMER_RIGHT=325,22
PRIMER_LEFT_TM=60.042
PRIMER_RIGHT_TM=60.208
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=0.2493
PRIMER_LEFT_1_PENALTY=0.041553
PRIMER_RIGHT_1_PENALTY=0.207756
PRIMER_LEFT_1_SEQUENCE=CTCAAGCGGTTATGCTTTCTCT
PRIMER_RIGHT_1_SEQUENCE=AGTATGTGGATGAAGAATCCCG
PRIMER_LEFT_1=204,22
PRIMER_RIGHT_1=325,22
PRIMER_LEFT_1_TM=60.042
PRIMER_RIGHT_1_TM=60.208
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=0.2833
PRIMER_LEFT_2_PENALTY=0.041553
PRIMER_RIGHT_2_PENALTY=0.241736
PRIMER_LEFT_2_SEQUENCE=CAAGCGGTTATGCTTTCTCTCT
PRIMER_RIGHT_2_SEQUENCE=GCAACAAATGCTGATTTCTCAC
PRIMER_LEFT_2=206,22
PRIMER_RIGHT_2=492,22
PRIMER_LEFT_2_TM=60.042
PRIMER_RIGHT_2_TM=59.758
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.2833
PRIMER_LEFT_3_PENALTY=0.041553
PRIMER_RIGHT_3_PENALTY=0.241736
PRIMER_LEFT_3_SEQUENCE=CTCAAGCGGTTATGCTTTCTCT
PRIMER_RIGHT_3_SEQUENCE=GCAACAAATGCTGATTTCTCAC
PRIMER_LEFT_3=204,22
PRIMER_RIGHT_3=492,22
PRIMER_LEFT_3_TM=60.042
PRIMER_RIGHT_3_TM=59.758
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=0.3165
PRIMER_LEFT_4_PENALTY=0.108707
PRIMER_RIGHT_4_PENALTY=0.207756
PRIMER_LEFT_4_SEQUENCE=GACAACCCCTCCTAACTAACCC
PRIMER_RIGHT_4_SEQUENCE=AGTATGTGGATGAAGAATCCCG
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=325,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=60.208
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_04113 Ps_contig_mira-and-tgicl-ass_franssen_32165
SEQUENCE=GAAGGACAGNGGTAGTCAACGCACGAGGTAACGCCGGGGGAAGGGTACGACAACCCCTCCTAACTAACCCCCTTTTCCTTAACCGAAATACCAGCTAATAAATAGAAAAGACGAAAACAAAAACAACACGCCGCNACTCTTATTTTCTTCTTCTCTTCCTCTTCTCTTAATCTAATCTAGAAAACAAAAATGAAGTCGGTAGAACTCAAGCGGTTATGCTTTCTCTCTTTATAACAATCGTAATATCAGAGAGGGTAACAGAGAGAGAAAGAAAGAATGGCTGTGGAATATCATTGTTGCGAACCGGGATTCTTCATCCACATACTCATAATCGTACTGTTGGTGGTGTTCGCCGGATTAATGTCCGGCCTCACCTTGGGACTCATGTCTTTAAGTCTCGTTGATCTTGAAGTTCTAGCTAAATCCGGTACTCCTCGCGATCGTAAACATGCCGAGAAAATATTGCCTGTTGTGAGAAATCAGCATTTGTTGCTTTGTACTTTGCTGATTTGCAATGCTGCCGCCATGGAGGCACTTCCAATTTTTCTTGATAGTCTTGTTACTGCTTGGGGTGCTATCCTTATTTCGGTCACGTTGATTCTTCTCTTTGGTGAGATTATACCCCAATCAATTTGTTCTCGATATGGTTTAGCTATTGGTGCATCAGTGACTCCCTTTGTTCGTGTGCTTGTATGGATATGTTACCCAGTTTCTTTTCCAATTAGCAAGTTGTTGGACTATTTGCTGGGTCATCGACACGAAGCTCTTTTCCGCAGAGCTGAGTTGAAAACGCTCGTAGATTTGCATGGTAATGAGGCTGGAAAAGGTGGGGAACTTACTCATGATGAAACAACAATAATTGCTGGGGCGCTTGAACTTAGTGAGAAAACAGCCAGTGATGCCATGACTCCTATATCTGAAATATTTGCCATTGATATTAATTCGAAGCTTGATAGGGGGCTGATGAACGAAATATTGGGCAAAGGGCATAGCAGAGTCCCAGTCTATTATGAGCAGTCCACAAACATTATTGGACTTATACTGATCAAGAATTTATTGACAATTCATCCGGAAGATGAAGAACCTGTGAAGAATGTAACGATACGCAGGATTCCAAGGGTTCCAGAAAGTATACCACTTTATGACATTTTGAATGAGTTTCAGAAGGGCCATAGTCACATGGCCGTTGTTGTAAGACAGTGTGACAAGAAGAAGCAACCATCTTCCCAAAATAATGCAAATGATTCGGTGAGAGAAGTGAAGGTGGATATTGATGGTGAAAAGCCTCCCCAAGAGAAAGTCTTGAAACCCAAGATACCAATCCAAAAGTGGAAAAGCTTTCCACATTCAAACAGGTCAAATAGGGGCAATTCTCGGGGCAGAAAATGGACAAAAAATATGTACTCAGATATTTTAGAGATAGATGGTAGTCCCCTTCCAAATATCCCTGAAGAAGAAGAAGCCATTGGAATAATTACAATGGAAGATGTCATTGAAGAACTTTTACAGGAGGAGATCTTTGATGAGACGGATCATCATTTTGAAGACTCATGACAATGGCTTTTCCATTAAACCTGATGTTTTCACATTCTTGTTTTGGACCCTGTAGATAATTAATTAGATAATGAGGATGAGTGTGAGAGCTTTTGTTTAGGGATCTTAAATGTTATTGTAAAGAACATATCAGTAATGAAATGTAGGCACAAGAAAGCAGTTATTACTTGTACGTCAAGCTTTCTTTATGGAGGTGTACATTAATGGATCTTTAAGGCAATGAAGCATGCCAGCAAATCGTGGTGTATGCCATTAAAGAAAA
TARGET=1450,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1261
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.117817
PRIMER_LEFT_SEQUENCE=CTTTCCACATTCAAACAGGTCA
PRIMER_RIGHT_SEQUENCE=GCCATTGTCATGAGTCTTCAAA
PRIMER_LEFT=1337,22
PRIMER_RIGHT=1560,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.118
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.1543
PRIMER_LEFT_1_PENALTY=0.036444
PRIMER_RIGHT_1_PENALTY=0.117817
PRIMER_LEFT_1_SEQUENCE=TAGTCCCCTTCCAAATATCCCT
PRIMER_RIGHT_1_SEQUENCE=GCCATTGTCATGAGTCTTCAAA
PRIMER_LEFT_1=1427,22
PRIMER_RIGHT_1=1560,22
PRIMER_LEFT_1_TM=60.036
PRIMER_RIGHT_1_TM=60.118
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=0.1577
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.117817
PRIMER_LEFT_2_SEQUENCE=AGCTTTCCACATTCAAACAGGT
PRIMER_RIGHT_2_SEQUENCE=GCCATTGTCATGAGTCTTCAAA
PRIMER_LEFT_2=1335,22
PRIMER_RIGHT_2=1560,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=60.118
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.1723
PRIMER_LEFT_3_PENALTY=0.054514
PRIMER_RIGHT_3_PENALTY=0.117817
PRIMER_LEFT_3_SEQUENCE=GAAACCCAAGATACCAATCCAA
PRIMER_RIGHT_3_SEQUENCE=GCCATTGTCATGAGTCTTCAAA
PRIMER_LEFT_3=1304,22
PRIMER_RIGHT_3=1560,22
PRIMER_LEFT_3_TM=60.055
PRIMER_RIGHT_3_TM=60.118
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.1723
PRIMER_LEFT_4_PENALTY=0.054514
PRIMER_RIGHT_4_PENALTY=0.117817
PRIMER_LEFT_4_SEQUENCE=TTGAAACCCAAGATACCAATCC
PRIMER_RIGHT_4_SEQUENCE=GCCATTGTCATGAGTCTTCAAA
PRIMER_LEFT_4=1302,22
PRIMER_RIGHT_4=1560,22
PRIMER_LEFT_4_TM=60.055
PRIMER_RIGHT_4_TM=60.118
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=259
=
PRIMER_SEQUENCE_ID=SSR_04114 Ps_contig_mira-and-tgicl-ass_franssen_32169
SEQUENCE=AAANTGAAGATAGAAAACTGTTGTATAACTTGTTTATAATAAAACCAATTAAATTAAATGTCTATATTGTACTGCAAAACAGGGTCAAAGACTCAAAACTCCAAGGACTTTTCTCTTGGAAACACAATACAAGCACATTGTCATTGATATTGATAATTGATAATATAATACATTGGAAATTACAGTCAACACCCTCCAAAAATACATGCAGTCCCAGCATGAGCTTATGTCCTTTAACCAGCAAATGAAGAGCCGTGAAACTCTAACAAGATCAAAAGGCATGAACAGCCAGCCAGCATGGGTCAAAGTTGTTACAAACACACAACCATAATCTTACAGATTGTGTTTTGAACCAAGAACAATGTATCAAACGAATGCATTCTAATCTGAATCAGATGACGATTGAGGAGGACACAATGTAGCAACCATTTTAGACAACATCTCCTGCATTTCCACCAGTTCTTCTTTCCCAAACCCCCAACTGCTAAGTAACTCTGCGTTGATACCACTCGCGTAATTCCATACCGGTGAAGATTTYGCAGTTTATTCTCCAAAAGTGGTAAAACAGCACTGCTGGAACGTAATCTGGCAGCCATGGGAATGGAATGGACGTCAAGGGATCCTTTTGATGAAGAATTAGTCAGTTGG
TARGET=286,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04115 Ps_contig_mira-and-tgicl-ass_franssen_32173
SEQUENCE=CCTTGAGACTTCAGATAAATTTGATACATATATAACTGAATTGTACTCAAGGGAAACATCAAATTCGTAGTATTTCGATATAAACAACAACAACAAACTGATTATGATTAGTCATTTTCTGTTTCTGCTAAAGAAAAAGTAGAATACAAACAAGAGTAACAGAAATGTAAAGGTGTTTTCTTTGTTACACATTCCACCTTACTCGTGTGGTATTTGAATGTATGCTGCATCACAGACAAAAGGGACATCGGCATACATACCAACAAGGGCACATCTAATGCACTCTATGGTAGTAAAGAGAAAAACAAAAAATGCAGTAGTCCAGAAATGCATTCCAAGTTTACCCCAATAAAACGAACGTGGCATCCAACGGCTCACAATCCCGGTAACCTGTAGGGCGATCTCGATTAACATTCCGACGGCGACATGGAATCGGAAGAAATGAGGCCATTCTTTTCTCCTCACAATTGTAAGATATGCAATCAAGAAGTATGCTATGAGAGACCATCTAGGTAGAGTTCCAATTGCCATGAGAAAAGGGTACGTCATGGGCTGGAAATACGGGATAAACGGGTGAAGATGGTATGCTGTCCGAGCATACATCCATGCCTGATGGAAAGGTAAGAGGTAAGGGATGCATGATAATGTTCTCCACCACCATCTTGGTTTTTTTGTCATCGGAGGGAATCGGAAGCCTGAGGAATCCTTTGTTGCCCGAGGGGTGGTAGAAGACTTGTGCAGCGTAGGTAACACGGGGATAGTACGAGACAGAAAATTCTGACCCCCGCTTAAAAGTAGGCTTGATGTAGCAGACATGTCTGTGAATGTCATTCCTCTTGGCTTATTTTCCAAACTATGTCCCCAGAAACTTCGGATACTTGAGACAGCAACCTTAGCAGGAATTTGACAAGCATAACACAAGACAGAACCTTGGGATACAGTGCCACCATTTTGAATCATGGTTGATTAACTCGGAAGTTTAGTCAGTGTTGTTATTTGAATTTTGAAAGTTTAGTCAGTGTTGTTGTTGTCGTTGTTGTTGTTCATGTTTAGGTTTTTGGGATTCACGTTTCATTGTTTTCCAGAGTAAGAAGAAGATAAACAATGTGTCTTGAATT
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.7396
PRIMER_LEFT_PENALTY=3.274160
PRIMER_RIGHT_PENALTY=0.465411
PRIMER_LEFT_SEQUENCE=CTGAATTGTACTCAAGGGAAACATC
PRIMER_RIGHT_SEQUENCE=CACACGAGTAAGGTGGAATGTG
PRIMER_LEFT=35,25
PRIMER_RIGHT=208,22
PRIMER_LEFT_TM=60.274
PRIMER_RIGHT_TM=60.465
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=3.9427
PRIMER_LEFT_1_PENALTY=3.274160
PRIMER_RIGHT_1_PENALTY=0.668546
PRIMER_LEFT_1_SEQUENCE=CTGAATTGTACTCAAGGGAAACATC
PRIMER_RIGHT_1_SEQUENCE=TGTGCCCTTGTTGGTATGTATG
PRIMER_LEFT_1=35,25
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=60.274
PRIMER_RIGHT_1_TM=60.669
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=4.2502
PRIMER_LEFT_2_PENALTY=3.274160
PRIMER_RIGHT_2_PENALTY=0.976051
PRIMER_LEFT_2_SEQUENCE=CTGAATTGTACTCAAGGGAAACATC
PRIMER_RIGHT_2_SEQUENCE=CCTTGTTGGTATGTATGCCGAT
PRIMER_LEFT_2=35,25
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=60.274
PRIMER_RIGHT_2_TM=60.976
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=9.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=4.4111
PRIMER_LEFT_3_PENALTY=3.274160
PRIMER_RIGHT_3_PENALTY=1.136955
PRIMER_LEFT_3_SEQUENCE=CTGAATTGTACTCAAGGGAAACATC
PRIMER_RIGHT_3_SEQUENCE=GAATGCATTTCTGGACTACTGC
PRIMER_LEFT_3=35,25
PRIMER_RIGHT_3=334,22
PRIMER_LEFT_3_TM=60.274
PRIMER_RIGHT_3_TM=58.863
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=4.4621
PRIMER_LEFT_4_PENALTY=3.274160
PRIMER_RIGHT_4_PENALTY=1.187985
PRIMER_LEFT_4_SEQUENCE=CTGAATTGTACTCAAGGGAAACATC
PRIMER_RIGHT_4_SEQUENCE=AAATACCACACGAGTAAGGTGGA
PRIMER_LEFT_4=35,25
PRIMER_RIGHT_4=214,23
PRIMER_LEFT_4_TM=60.274
PRIMER_RIGHT_4_TM=59.812
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=180
=
PRIMER_SEQUENCE_ID=SSR_04116 Ps_contig_mira-and-tgicl-ass_franssen_32173
SEQUENCE=CCTTGAGACTTCAGATAAATTTGATACATATATAACTGAATTGTACTCAAGGGAAACATCAAATTCGTAGTATTTCGATATAAACAACAACAACAAACTGATTATGATTAGTCATTTTCTGTTTCTGCTAAAGAAAAAGTAGAATACAAACAAGAGTAACAGAAATGTAAAGGTGTTTTCTTTGTTACACATTCCACCTTACTCGTGTGGTATTTGAATGTATGCTGCATCACAGACAAAAGGGACATCGGCATACATACCAACAAGGGCACATCTAATGCACTCTATGGTAGTAAAGAGAAAAACAAAAAATGCAGTAGTCCAGAAATGCATTCCAAGTTTACCCCAATAAAACGAACGTGGCATCCAACGGCTCACAATCCCGGTAACCTGTAGGGCGATCTCGATTAACATTCCGACGGCGACATGGAATCGGAAGAAATGAGGCCATTCTTTTCTCCTCACAATTGTAAGATATGCAATCAAGAAGTATGCTATGAGAGACCATCTAGGTAGAGTTCCAATTGCCATGAGAAAAGGGTACGTCATGGGCTGGAAATACGGGATAAACGGGTGAAGATGGTATGCTGTCCGAGCATACATCCATGCCTGATGGAAAGGTAAGAGGTAAGGGATGCATGATAATGTTCTCCACCACCATCTTGGTTTTTTTGTCATCGGAGGGAATCGGAAGCCTGAGGAATCCTTTGTTGCCCGAGGGGTGGTAGAAGACTTGTGCAGCGTAGGTAACACGGGGATAGTACGAGACAGAAAATTCTGACCCCCGCTTAAAAGTAGGCTTGATGTAGCAGACATGTCTGTGAATGTCATTCCTCTTGGCTTATTTTCCAAACTATGTCCCCAGAAACTTCGGATACTTGAGACAGCAACCTTAGCAGGAATTTGACAAGCATAACACAAGACAGAACCTTGGGATACAGTGCCACCATTTTGAATCATGGTTGATTAACTCGGAAGTTTAGTCAGTGTTGTTATTTGAATTTTGAAAGTTTAGTCAGTGTTGTTGTTGTCGTTGTTGTTGTTCATGTTTAGGTTTTTGGGATTCACGTTTCATTGTTTTCCAGAGTAAGAAGAAGATAAACAATGTGTCTTGAATT
TARGET=1020,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3472
PRIMER_LEFT_PENALTY=0.099438
PRIMER_RIGHT_PENALTY=0.247794
PRIMER_LEFT_SEQUENCE=ACACAAGACAGAACCTTGGGAT
PRIMER_RIGHT_SEQUENCE=CGTGAATCCCAAAAACCTAAAC
PRIMER_LEFT=915,22
PRIMER_RIGHT=1068,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=59.752
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.3000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.3807
PRIMER_LEFT_1_PENALTY=0.132857
PRIMER_RIGHT_1_PENALTY=0.247794
PRIMER_LEFT_1_SEQUENCE=TACAGTGCCACCATTTTGAATC
PRIMER_RIGHT_1_SEQUENCE=CGTGAATCCCAAAAACCTAAAC
PRIMER_LEFT_1=936,22
PRIMER_RIGHT_1=1068,22
PRIMER_LEFT_1_TM=59.867
PRIMER_RIGHT_1_TM=59.752
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=0.3807
PRIMER_LEFT_2_PENALTY=0.132857
PRIMER_RIGHT_2_PENALTY=0.247794
PRIMER_LEFT_2_SEQUENCE=GATACAGTGCCACCATTTTGAA
PRIMER_RIGHT_2_SEQUENCE=CGTGAATCCCAAAAACCTAAAC
PRIMER_LEFT_2=934,22
PRIMER_RIGHT_2=1068,22
PRIMER_LEFT_2_TM=59.867
PRIMER_RIGHT_2_TM=59.752
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=135
PRIMER_PAIR_PENALTY_3=0.3838
PRIMER_LEFT_3_PENALTY=0.135999
PRIMER_RIGHT_3_PENALTY=0.247794
PRIMER_LEFT_3_SEQUENCE=GCAACCTTAGCAGGAATTTGAC
PRIMER_RIGHT_3_SEQUENCE=CGTGAATCCCAAAAACCTAAAC
PRIMER_LEFT_3=886,22
PRIMER_RIGHT_3=1068,22
PRIMER_LEFT_3_TM=60.136
PRIMER_RIGHT_3_TM=59.752
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.4506
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.351175
PRIMER_LEFT_4_SEQUENCE=ACACAAGACAGAACCTTGGGAT
PRIMER_RIGHT_4_SEQUENCE=CTCTGGAAAACAATGAAACGTG
PRIMER_LEFT_4=915,22
PRIMER_RIGHT_4=1086,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=59.649
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.3000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_04117 Ps_contig_mira-and-tgicl-ass_franssen_32173
SEQUENCE=CCTTGAGACTTCAGATAAATTTGATACATATATAACTGAATTGTACTCAAGGGAAACATCAAATTCGTAGTATTTCGATATAAACAACAACAACAAACTGATTATGATTAGTCATTTTCTGTTTCTGCTAAAGAAAAAGTAGAATACAAACAAGAGTAACAGAAATGTAAAGGTGTTTTCTTTGTTACACATTCCACCTTACTCGTGTGGTATTTGAATGTATGCTGCATCACAGACAAAAGGGACATCGGCATACATACCAACAAGGGCACATCTAATGCACTCTATGGTAGTAAAGAGAAAAACAAAAAATGCAGTAGTCCAGAAATGCATTCCAAGTTTACCCCAATAAAACGAACGTGGCATCCAACGGCTCACAATCCCGGTAACCTGTAGGGCGATCTCGATTAACATTCCGACGGCGACATGGAATCGGAAGAAATGAGGCCATTCTTTTCTCCTCACAATTGTAAGATATGCAATCAAGAAGTATGCTATGAGAGACCATCTAGGTAGAGTTCCAATTGCCATGAGAAAAGGGTACGTCATGGGCTGGAAATACGGGATAAACGGGTGAAGATGGTATGCTGTCCGAGCATACATCCATGCCTGATGGAAAGGTAAGAGGTAAGGGATGCATGATAATGTTCTCCACCACCATCTTGGTTTTTTTGTCATCGGAGGGAATCGGAAGCCTGAGGAATCCTTTGTTGCCCGAGGGGTGGTAGAAGACTTGTGCAGCGTAGGTAACACGGGGATAGTACGAGACAGAAAATTCTGACCCCCGCTTAAAAGTAGGCTTGATGTAGCAGACATGTCTGTGAATGTCATTCCTCTTGGCTTATTTTCCAAACTATGTCCCCAGAAACTTCGGATACTTGAGACAGCAACCTTAGCAGGAATTTGACAAGCATAACACAAGACAGAACCTTGGGATACAGTGCCACCATTTTGAATCATGGTTGATTAACTCGGAAGTTTAGTCAGTGTTGTTATTTGAATTTTGAAAGTTTAGTCAGTGTTGTTGTTGTCGTTGTTGTTGTTCATGTTTAGGTTTTTGGGATTCACGTTTCATTGTTTTCCAGAGTAAGAAGAAGATAAACAATGTGTCTTGAATT
TARGET=1033,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3472
PRIMER_LEFT_PENALTY=0.099438
PRIMER_RIGHT_PENALTY=0.247794
PRIMER_LEFT_SEQUENCE=ACACAAGACAGAACCTTGGGAT
PRIMER_RIGHT_SEQUENCE=CGTGAATCCCAAAAACCTAAAC
PRIMER_LEFT=915,22
PRIMER_RIGHT=1068,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=59.752
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.3000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.3807
PRIMER_LEFT_1_PENALTY=0.132857
PRIMER_RIGHT_1_PENALTY=0.247794
PRIMER_LEFT_1_SEQUENCE=TACAGTGCCACCATTTTGAATC
PRIMER_RIGHT_1_SEQUENCE=CGTGAATCCCAAAAACCTAAAC
PRIMER_LEFT_1=936,22
PRIMER_RIGHT_1=1068,22
PRIMER_LEFT_1_TM=59.867
PRIMER_RIGHT_1_TM=59.752
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=0.3807
PRIMER_LEFT_2_PENALTY=0.132857
PRIMER_RIGHT_2_PENALTY=0.247794
PRIMER_LEFT_2_SEQUENCE=GATACAGTGCCACCATTTTGAA
PRIMER_RIGHT_2_SEQUENCE=CGTGAATCCCAAAAACCTAAAC
PRIMER_LEFT_2=934,22
PRIMER_RIGHT_2=1068,22
PRIMER_LEFT_2_TM=59.867
PRIMER_RIGHT_2_TM=59.752
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=135
PRIMER_PAIR_PENALTY_3=0.3838
PRIMER_LEFT_3_PENALTY=0.135999
PRIMER_RIGHT_3_PENALTY=0.247794
PRIMER_LEFT_3_SEQUENCE=GCAACCTTAGCAGGAATTTGAC
PRIMER_RIGHT_3_SEQUENCE=CGTGAATCCCAAAAACCTAAAC
PRIMER_LEFT_3=886,22
PRIMER_RIGHT_3=1068,22
PRIMER_LEFT_3_TM=60.136
PRIMER_RIGHT_3_TM=59.752
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.4506
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.351175
PRIMER_LEFT_4_SEQUENCE=ACACAAGACAGAACCTTGGGAT
PRIMER_RIGHT_4_SEQUENCE=CTCTGGAAAACAATGAAACGTG
PRIMER_LEFT_4=915,22
PRIMER_RIGHT_4=1086,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=59.649
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.3000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_04118 Ps_contig_mira-and-tgicl-ass_franssen_32174
SEQUENCE=AAGTTTTATTCTCAAGATTTAATAATCATAAATCTAACAATTTTATTGACATGAAAAATGGGAGTTTAAAATGCAATGTTTGTGATGCCAATATTACCAAACGCAAAATATCACTCAAGCTGGCACATCCCCAGCTAATAACACTTCTGGAATTTAGAGGGGCGAGGAAATAAAGTTTGATTTTAGAAGCAAACAAGTGAGGCAAAAAATGTCTTAAAATTGGATGATTCTACCCTAATTTATGATAAAGAAAGGAAAGATTAATGGGTTCTACTACTTGGCCTGAGGATGAGATGCAATGAAGTCAATTGCAAGATTAATGGAGTTAATCTTGTCTGCTTCATTATCAGGAATCTCAAATCCAAACTCTTCCTCAAGAGCCATCACAACCTCTACAGCATCTAAACTATCCAATCCAAGGTCGTTCTGGAAATGAGCAGATGGAGTAACCTTAGAAGGATCTACTTTCTGGAAGTTTTTGACGCACGAAACGACGCGATCTGTGACCTCAGATTTATCGAGGAAAGAACCCCTAACCTCTTCGCTGAAGCGACGGAGGAAGAAATTGGGAAGAAGACGCAGAGGAGACTGGTGGTTGAGCGGAAGAGTATCAACCTGAACTCTTAGGTATTTCATCAAAGGCAAACCGGTTCTCCTCGCCGCCATTGCTGATATCAAAAACCTCACTCTCTCTCTCTGTTCAAATCTACACAACGAAGTGAAAAGAANNATTGTGAAGTTAGAT
TARGET=687,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04119 Ps_contig_mira-and-tgicl-ass_franssen_32177
SEQUENCE=CCGGAACGGTAAGGTAAACGGTACCGGGGGGGGTAACGGGGGTAACGGGGTAAGGTTAAGGGTTAGGTACGGTCCGTAACGTAACCTTACGTAAGAAAAACGAAAACGAAATAGCTTCTTCTACCTAACCCTAACTTCTCACTCCTCTCTCTTCTTCGCTTAAACCTAATCCTCCTTCTTCTTCTTCTTGTAACTATCGATTACCGTCTATCATCTCTATCTCTTCCCGTTCGCGTTTTTCGAGGCATGTTGTTCGTATGGCTCCTGAAGACGAGAAGTTAGTCCGTCGTTCGCCTCTCGATTTCCCAATCGAGTGGGAGAGGCCTAAGCCAGGACGAAGATCAGATATATTCCCTCAGTTTAGTCCGATGAAGACACCTTTGCCACCTCCAATGCCTGCAGATCCTCCGGAAGAGGATGAAGAAAATGAAGAGGAAGAGGAAGATCCTGAAAAGGAGGAGCCAGAGCAGCAATAGTGTAGTAAATGACTTGTCTTGAATTTTGCAGTTATCTTGATATGTCAACTAGGGTAGTGCTGCTTGCTGAACTTAATGTAATAGTAGGCCATTATATGAATAACAAATGATGTTTGAGCTTTCTTCGATTTTTCGACCAGCTGTATTCGCCTGTATACCATGAGTTTAATTTACCATGCAAAAAATGAAAATTGTACGCATCACTGATATTTGGTTGGGGTAGTATACCAAAGTATATTTTCCTCTACGGTAAACCTTTCCTGCAGTTTTGGTGGACCTTGATCTATAATTTTTAAGATAACC
TARGET=175,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0202
PRIMER_LEFT_PENALTY=0.001341
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=TAACGGGGTAAGGTTAAGGGTT
PRIMER_RIGHT_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_LEFT=42,22
PRIMER_RIGHT=280,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.0202
PRIMER_LEFT_1_PENALTY=0.001341
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=AACGGGGTAAGGTTAAGGGTTA
PRIMER_RIGHT_1_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_LEFT_1=43,22
PRIMER_RIGHT_1=280,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.0235
PRIMER_LEFT_2_PENALTY=0.004697
PRIMER_RIGHT_2_PENALTY=0.018835
PRIMER_LEFT_2_SEQUENCE=GGTTAAGGGTTAGGTACGGTCC
PRIMER_RIGHT_2_SEQUENCE=TAACTTCTCGTCTTCAGGAGCC
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=280,22
PRIMER_LEFT_2_TM=60.005
PRIMER_RIGHT_2_TM=60.019
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=228
PRIMER_PAIR_PENALTY_3=0.0582
PRIMER_LEFT_3_PENALTY=0.001341
PRIMER_RIGHT_3_PENALTY=0.056850
PRIMER_LEFT_3_SEQUENCE=TAACGGGGTAAGGTTAAGGGTT
PRIMER_RIGHT_3_SEQUENCE=CGACGGACTAACTTCTCGTCTT
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=288,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.0582
PRIMER_LEFT_4_PENALTY=0.001341
PRIMER_RIGHT_4_PENALTY=0.056850
PRIMER_LEFT_4_SEQUENCE=AACGGGGTAAGGTTAAGGGTTA
PRIMER_RIGHT_4_SEQUENCE=CGACGGACTAACTTCTCGTCTT
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=288,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.943
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_04120 Ps_contig_mira-and-tgicl-ass_franssen_32183
SEQUENCE=GTATAGTATGTAATAAGTTATGTTTTCTGCGTATAACGACCTGAAGGATACATTGTATATATTACTATTTTTTTGTCCACAAAATATTTAGCTGCGAATCATGAATACTAAAATCATAAGTTAGAACCCTTACGTGATTTGAGAAACTTGATTAGAGATGGTAATGTCATCTTCTTATTACAAACATTACCGCGAACATCAAATTACACACATGAGATATACAAAAGGTGACAAACTATATAATGCACTCACATCAAAAGCTAGTTTCTGCTGTCACTTCAAGATTTTGGATTCAATGTCTTCTAAGCTCAAGCCTTTTGTTTCCGGGACAGACGTTACAATGAACACAAGTGACACTAAAGCAATGGCCGCAAAAAGGAGAAAAAGGTTATCTGCTCCTAGATACTCCTTTAGTGGTGAGAATGCAAAGGTAACAACAGCATTGGCAGCGAAGTTGGTAAGAACAGCCATACTAATCCCCCGTCCTCTAGTGCGAAGTGGGAAGATTTCCGATACCATAAGCCAACTTATCGGCCCAAACGATATCTGGTAGCAACCAACGTAAAGAAGTAAAGCGGCAACAGCAACAATTGGTAACCCTCCGAGGAATTTATAATAAGCGGAAAGGAGAACTAAAGATAGGGCAATGCCACTGACACCTCCAATCAGAAGAGGTCTTCTCCCCAAATCATCTACTTTTAGAACAGCAACCGATGTCATTACTAACTTGAATAAGCCAATAACAACTGAAACTTTAGCCGCGTCGGCTGCGGCAGAAAATCCAGCACTCTGAAGAATAGGACCTGCATAATACAGAACACTAGGCTGACCTGTTATCTGTTGAAATAATACTAGCCCCCCACCGATTATGAACGCTTTCAGATTGGGACCCTGAAACACCTCTAAGAAATTCGCCTCGGATTCTTGATCTTCATACGCTGACTTCAATGAGACGATACTCTCTTCTATCTGCTTCTCAGATTCTTTGTCACCGGGTGGTCGGCCTCTTAATTTGCTAAGGGAAACAATTGCCTTCTCCTTCAAATCTTGAAAAGAACCTTTTCCTTGTACTGCTTTGAGAAGCAACCATCGAGGAGAGGCGGGGAGAGTCCACATTCCGAGCCCCATTAACACAGCAAGCGGTGCACTAAATCCATACATGAATCGCCATCCACCAACTGTAGTAATCTGAAAGCTTCCCACAAAATAACCCAACAGAATACCCAACACAATGAAGAGTTCTTTTAATGATACTAGAGTCCCGCGGATTTGAGATGGACATGTTTCTGCAATGTATAAAGGAGCTCCATGCATGGCCAGACCTATACCAAGTCCATAAATCAGCCTTCCTGCTAAGAGAACACCAAGTTCCGGTGCAGTGGCAGTGATTGCACTGCCAAGTAGATACAACAAGGCCGCGCCGATTAGCTGTCGCTTCCTCCCAATGAAATCAGCAATTGCAAAAGCAAGAAGTGAGCCAAGAAGAGCCCCATATAGGGAACCACTGACCACAAGACCAAGCTGAACGGCAGAAAGATTGGACCAAGCGATACCGCTAAGCTCCGGTGACTGCAGCGAAATTGTAGCACCGGACGTGGCACCAATATCATAACCAAATAACAAGCCACCCAATGCAGGGAACACAAACGGAAGAATCACAGAAGACCAAGAAAATTCCTCTTGGTAAGTTTCATCAGAAGCAACCGACTCAGAATTGCTCCCAGCAGAATACTCCTTCTGCGCTTGAACATGGAATTTGCGGTTATGAATAGAGTGAGAGGTGAGAAGTGGAAAACAGAGACGTGTTGGCGAGGAATGATGGTTAATGGAAGAAGAAAGGAAGCGCGGAGAAGAAAACGGAGGTTTTGACTTTGTTATTATTCTTTGATGGTGAAGTGGTTTGAAATTGAAGAGAGTAGTTGATGATGCCATTTTGAGACTTGAGAGTAGTTGGTTGAATCAGGAGTGAGGAATCATGGTTGTTTGTTTGTTTGTTTCYNYNAGTGAATCTTGACAAGTAAATGAAACGGCACAGT
TARGET=1980,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04121 Ps_contig_mira-and-tgicl-ass_franssen_32185
SEQUENCE=ATTATTTCTCTTTTATATNATTATCATCATCATAATTAATATCCTATCCTATCATAAAGAAACACAACTTTTACTTCAAATTAATCAACTCATTCATTCTCTTCCAACATAGAACAAACAACAACACAAACACAAACAACCTTGCCGTTGATGGAAAATTGTCTTCACCACCTTACAATCAAAGGATTCTTCTTCACAAAACCTATTTCTTTTTCATCTTCTTCTTCTTCTTCTTTGAAATTTAGGTGCAACAACAACAGCACCAAATTGAAGGTGATTAAGGCGAGTGCCGGAGCTAGCCACTGTCAGTTCAGTAGTTTGAACTCTCCTTTGTTACCACAATCCACGGTTGGAAAGTTTCTTAGTGGTGTTTTGCAGAATCATCGTAATCTCTTTCATGTTGCTGTTCAAGAAGAACTTAAACTATTGGCTGATGATCGAGATGCTGCGGTTTCTCGCATGATCCTTAGTTCTGATTCCGATCAAGCCTTGCTTCACAGGAGGATTGCACAAATAAAAGAGAATCAATGTGAAGTTGCTGTAGAAGATGTTATGTCCTTGTTGATATTTTACAAGTTTTCCGAAATCCGGGTTCCTTTGGTTCCAAAGCTTTCAAGTTGTCTTTACAATGGAAGGTTAGAGATATTGCCTTCTAAAGATTGGGAGCTAGAGTCTATTCACACTTTAGAAGTTTTGGATATGATAAGGGAACACATAACCACCGTGACCGGCTTGAAAGCAAAACCTAGTGTAACAGAAAGTTGGGCAACTACCAAAGTTCGACAATTCTTACTCGCGCGTGTATATGTCGCTTCAATATTATACGGCTATTTTTTGAAGTCGGTTTCCTTAAGGTATCATCTAGAAAGAAACCTAAATTTGGCTAACAATGATGTTCATCTTGGTCATAGGAGTACTAACCTCTCATTTAAAGATATGTATCCTTACGGATTCGAGGAAGACATATTTGGTCATTTGAGTAACATGCAATCGATCGGGCACGGGCACGGGCAAGGGCTAATTCGATACGAAGAGGAAATTGAAGATTTGAAGTGCTATGTTATGAAGTTTCACCCTGGATCATTGCAGAGATGTGCTAAGTTGAGATCTAAGGAGGCTGTGAATTTGGTTAGGAGTTATAGTTGTGCGCTTTTTAACAATGAAGGGTTTGATTCTGTTGAGAATGATGATGTTATTTTGACTTCATTTTCAAGTTTGAAGAGACTAGTCTTGGAAGCTGTTGCTTTTGGATCATTCTTGTGGGAAACTGAAGATTACATTGACAATGTATATAAGCTTAAGGATCATTAAGATAGAAGTAAGCAATCATTTTGATCCTTGACTGTGGCGAGTACTATCGTTACGTGTCTTCTGTTACTCAGATTGGTCCTAACGGATAATAGAATCAATGTTGTTTTCGATATTTTGATGCATTAAGAGACTATAGTAACACTGACTCGAATATTAAGGTACTAAAGTGAAGAAGCGGAGTAAGTTCCAATGCTCATTGAGCGAATTCCTATTGTGTATATAGTGAGTTTCGATTTTATATTTTGAGTGATAATTTGGTTATGTTTGTATTGTATAGTACACATTTTTATTTACATTGTACAATGTGTATATGTATCTATATATATGTTTTGAATATTTTCCAGATTTTTTTATGATGGTTATGATATGATGTAATTAGAGAGAAGAAATATGTTTTCTTATTTGTACAGAAATAGAGATGAGTTTAAAGACTCATCAATTATTTAACTGCATGATTGAAGAATCTTGTAAGTCTAGGTGATTTAATATTTCGAACCCTCTAATAGTATTTGCCATAAAGTGTAG
TARGET=217,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0485
PRIMER_LEFT_PENALTY=0.019966
PRIMER_RIGHT_PENALTY=0.028536
PRIMER_LEFT_SEQUENCE=ACACAAACACAAACAACCTTGC
PRIMER_RIGHT_SEQUENCE=CTAAGAAACTTTCCAACCGTGG
PRIMER_LEFT=123,22
PRIMER_RIGHT=364,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.029
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.0485
PRIMER_LEFT_1_PENALTY=0.019966
PRIMER_RIGHT_1_PENALTY=0.028536
PRIMER_LEFT_1_SEQUENCE=ACACAAACACAAACAACCTTGC
PRIMER_RIGHT_1_SEQUENCE=CCACTAAGAAACTTTCCAACCG
PRIMER_LEFT_1=123,22
PRIMER_RIGHT_1=367,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=60.029
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.1764
PRIMER_LEFT_2_PENALTY=0.019966
PRIMER_RIGHT_2_PENALTY=0.156479
PRIMER_LEFT_2_SEQUENCE=ACACAAACACAAACAACCTTGC
PRIMER_RIGHT_2_SEQUENCE=GCCTTAATCACCTTCAATTTGG
PRIMER_LEFT_2=123,22
PRIMER_RIGHT_2=283,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=59.844
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=0.2521
PRIMER_LEFT_3_PENALTY=0.095574
PRIMER_RIGHT_3_PENALTY=0.156479
PRIMER_LEFT_3_SEQUENCE=ATGGAAAATTGTCTTCACCACC
PRIMER_RIGHT_3_SEQUENCE=GCCTTAATCACCTTCAATTTGG
PRIMER_LEFT_3=150,22
PRIMER_RIGHT_3=283,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.844
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=134
PRIMER_PAIR_PENALTY_4=0.4049
PRIMER_LEFT_4_PENALTY=0.095574
PRIMER_RIGHT_4_PENALTY=0.309288
PRIMER_LEFT_4_SEQUENCE=ATGGAAAATTGTCTTCACCACC
PRIMER_RIGHT_4_SEQUENCE=CATCTCGATCATCAGCCAATAG
PRIMER_LEFT_4=150,22
PRIMER_RIGHT_4=444,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=59.691
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_04122 Ps_contig_mira-and-tgicl-ass_franssen_32195
SEQUENCE=AAACATATAATCTCAACCAAACGCGTCACTCTTTTATTAGTTTTTCAAGATTAGAAGTGAACGTCACAGATTTATCTCCATTTTCTAGATAAATTCACTCATTCATTCATTCTTCTCTCTCAAAAATCCCTTTATCCTCCTTCAACTCTTTCAATAATGGCTGTGGCTATGGCTGCTTGTAGCCTTGGACTTCACCTCACTCCTGATAATAATAATAATAATACTAATTGCTTTGCCCCTATCTTGAAAACCACTGCTATGTATGATACTAGTAAACCATGTTTCATTCTTCACAAAACAAGATTATTATCTTCAGCAATTACGATCGTCCCTCGAGCAACAGCTGTTTCTAATGTCGAGGATGGAAATCAAAGTGTGGCTGATACTGTCCCAACTCCTGTTGTTATAATTGATCAAGATTCTGATCCGGACGCAACTGTCGTCGAGATTACCTTTGGTGATCGCCTTGGCGCTCTTCTCGACACTATGAATGCATTGAAAAACTTGGGACTGAATGTTGTTAAGGCTAATGTGTATCTTGATTCCTCTGGCAAGCACAACAAGTTTTCAATCACAAAAGCTGATACAGGTAGGAAAGTGGAAGATCCAGAGTTGTTAGAAGCGATCCGTTTGACGATTTTAAATAACATGATCACGTATCACCCGGAATCAAGTGCACAATTGGCTCTAGGAGCGGCTTTTGGACTTGTGGCTCCAAAGGAACAGGTTGATGTGGACATAGCAACTCACTTAACCATTGCTGATGACGGTCCAGAGAGAAGTTTGTTCTATGTGGAGACCGCTGATCGCCCTGGATTATTGGTCGATCTTGTAAAGGGTATTACTGACATTAACATTGCTGTTGAATCCGGGGAATTCGACACTGAGGGGCTGTTGGCTAAGGCAAAGTTTCATGTCAGCTACAATGGTAAAGCCATCATCAAGCCTCTTCAGCAGGTTCTTGTTAACAGTTTGAGATACTTCTTGACACGGCCTTCAACTGAGGAATCCAGTTTTTGAGTGGCACATTGTCACAACCAATTGCAACACATTGTATTACTCACTCTCGTCTATAATATTTTGTTGTTGCATTTAATCATTTAACACAGAGATAAAAAAAAATATAGAAAATCGGGTTTTACTGATTTACCTTTATTAACTC
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0766
PRIMER_LEFT_PENALTY=0.061818
PRIMER_RIGHT_PENALTY=0.014792
PRIMER_LEFT_SEQUENCE=ACCAAACGCGTCACTCTTTTAT
PRIMER_RIGHT_SEQUENCE=CATAGCCACAGCCATTATTGAA
PRIMER_LEFT=15,22
PRIMER_RIGHT=170,22
PRIMER_LEFT_TM=60.062
PRIMER_RIGHT_TM=59.985
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=0.2363
PRIMER_LEFT_1_PENALTY=0.061818
PRIMER_RIGHT_1_PENALTY=0.174518
PRIMER_LEFT_1_SEQUENCE=ACCAAACGCGTCACTCTTTTAT
PRIMER_RIGHT_1_SEQUENCE=GTGAGGTGAAGTCCAAGGCTAC
PRIMER_LEFT_1=15,22
PRIMER_RIGHT_1=199,22
PRIMER_LEFT_1_TM=60.062
PRIMER_RIGHT_1_TM=60.175
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.2513
PRIMER_LEFT_2_PENALTY=0.061818
PRIMER_RIGHT_2_PENALTY=0.189529
PRIMER_LEFT_2_SEQUENCE=ACCAAACGCGTCACTCTTTTAT
PRIMER_RIGHT_2_SEQUENCE=AGGTGAAGTCCAAGGCTACAAG
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=196,22
PRIMER_LEFT_2_TM=60.062
PRIMER_RIGHT_2_TM=59.810
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=182
PRIMER_PAIR_PENALTY_3=0.3637
PRIMER_LEFT_3_PENALTY=0.061818
PRIMER_RIGHT_3_PENALTY=0.301926
PRIMER_LEFT_3_SEQUENCE=ACCAAACGCGTCACTCTTTTAT
PRIMER_RIGHT_3_SEQUENCE=AGTGAGGTGAAGTCCAAGGCTA
PRIMER_LEFT_3=15,22
PRIMER_RIGHT_3=200,22
PRIMER_LEFT_3_TM=60.062
PRIMER_RIGHT_3_TM=60.302
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=0.4089
PRIMER_LEFT_4_PENALTY=0.061818
PRIMER_RIGHT_4_PENALTY=0.347129
PRIMER_LEFT_4_SEQUENCE=ACCAAACGCGTCACTCTTTTAT
PRIMER_RIGHT_4_SEQUENCE=AGCAGTGGTTTTCAAGATAGGG
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=60.062
PRIMER_RIGHT_4_TM=59.653
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_04123 Ps_contig_mira-and-tgicl-ass_franssen_32195
SEQUENCE=AAACATATAATCTCAACCAAACGCGTCACTCTTTTATTAGTTTTTCAAGATTAGAAGTGAACGTCACAGATTTATCTCCATTTTCTAGATAAATTCACTCATTCATTCATTCTTCTCTCTCAAAAATCCCTTTATCCTCCTTCAACTCTTTCAATAATGGCTGTGGCTATGGCTGCTTGTAGCCTTGGACTTCACCTCACTCCTGATAATAATAATAATAATACTAATTGCTTTGCCCCTATCTTGAAAACCACTGCTATGTATGATACTAGTAAACCATGTTTCATTCTTCACAAAACAAGATTATTATCTTCAGCAATTACGATCGTCCCTCGAGCAACAGCTGTTTCTAATGTCGAGGATGGAAATCAAAGTGTGGCTGATACTGTCCCAACTCCTGTTGTTATAATTGATCAAGATTCTGATCCGGACGCAACTGTCGTCGAGATTACCTTTGGTGATCGCCTTGGCGCTCTTCTCGACACTATGAATGCATTGAAAAACTTGGGACTGAATGTTGTTAAGGCTAATGTGTATCTTGATTCCTCTGGCAAGCACAACAAGTTTTCAATCACAAAAGCTGATACAGGTAGGAAAGTGGAAGATCCAGAGTTGTTAGAAGCGATCCGTTTGACGATTTTAAATAACATGATCACGTATCACCCGGAATCAAGTGCACAATTGGCTCTAGGAGCGGCTTTTGGACTTGTGGCTCCAAAGGAACAGGTTGATGTGGACATAGCAACTCACTTAACCATTGCTGATGACGGTCCAGAGAGAAGTTTGTTCTATGTGGAGACCGCTGATCGCCCTGGATTATTGGTCGATCTTGTAAAGGGTATTACTGACATTAACATTGCTGTTGAATCCGGGGAATTCGACACTGAGGGGCTGTTGGCTAAGGCAAAGTTTCATGTCAGCTACAATGGTAAAGCCATCATCAAGCCTCTTCAGCAGGTTCTTGTTAACAGTTTGAGATACTTCTTGACACGGCCTTCAACTGAGGAATCCAGTTTTTGAGTGGCACATTGTCACAACCAATTGCAACACATTGTATTACTCACTCTCGTCTATAATATTTTGTTGTTGCATTTAATCATTTAACACAGAGATAAAAAAAAATATAGAAAATCGGGTTTTACTGATTTACCTTTATTAACTC
TARGET=206,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3344
PRIMER_LEFT_PENALTY=0.014792
PRIMER_RIGHT_PENALTY=0.319597
PRIMER_LEFT_SEQUENCE=TTCAATAATGGCTGTGGCTATG
PRIMER_RIGHT_SEQUENCE=CTTTGATTTCCATCCTCGACAT
PRIMER_LEFT=149,22
PRIMER_RIGHT=373,22
PRIMER_LEFT_TM=59.985
PRIMER_RIGHT_TM=60.320
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.3528
PRIMER_LEFT_1_PENALTY=0.014792
PRIMER_RIGHT_1_PENALTY=0.338015
PRIMER_LEFT_1_SEQUENCE=TTCAATAATGGCTGTGGCTATG
PRIMER_RIGHT_1_SEQUENCE=GGACAGTATCAGCCACACTTTG
PRIMER_LEFT_1=149,22
PRIMER_RIGHT_1=390,22
PRIMER_LEFT_1_TM=59.985
PRIMER_RIGHT_1_TM=59.662
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.3619
PRIMER_LEFT_2_PENALTY=0.014792
PRIMER_RIGHT_2_PENALTY=0.347129
PRIMER_LEFT_2_SEQUENCE=TTCAATAATGGCTGTGGCTATG
PRIMER_RIGHT_2_SEQUENCE=AGCAGTGGTTTTCAAGATAGGG
PRIMER_LEFT_2=149,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=59.985
PRIMER_RIGHT_2_TM=59.653
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=0.4089
PRIMER_LEFT_3_PENALTY=0.061818
PRIMER_RIGHT_3_PENALTY=0.347129
PRIMER_LEFT_3_SEQUENCE=ACCAAACGCGTCACTCTTTTAT
PRIMER_RIGHT_3_SEQUENCE=AGCAGTGGTTTTCAAGATAGGG
PRIMER_LEFT_3=15,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=60.062
PRIMER_RIGHT_3_TM=59.653
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.4941
PRIMER_LEFT_4_PENALTY=0.174518
PRIMER_RIGHT_4_PENALTY=0.319597
PRIMER_LEFT_4_SEQUENCE=GTAGCCTTGGACTTCACCTCAC
PRIMER_RIGHT_4_SEQUENCE=CTTTGATTTCCATCCTCGACAT
PRIMER_LEFT_4=178,22
PRIMER_RIGHT_4=373,22
PRIMER_LEFT_4_TM=60.175
PRIMER_RIGHT_4_TM=60.320
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=196
=
PRIMER_SEQUENCE_ID=SSR_04124 Ps_contig_mira-and-tgicl-ass_franssen_32197
SEQUENCE=AGGCTAGGCTGTAGCTGAAATGAAAATACGCGTTGCGAATGATACGAGACGACTTTATATATAAATGAGAATGCAAGTAGTAANNAGGGTGACATCAAAGTAGAAGATCAACCGCATCGCACAAGTACCAACAACAACATGAAAAACAATTCCTATCATTTCTCTACTTGAAACCCTAACTCACTCCGTTCTCGATCTGCAACTACCCATTTTCCAACCCCGTCTTCTGTCTCCATCTGTACACAGTTACAGATCCGTTTTTGTAGAATGGATGGAAACAAAGACGATGCATTGAAATCCTTCAAAATCGGTAAAGAAGCGTTGCAAAACGGCGATCGGAATCGCGCTTTGAAGTTTCTTAACAAAGCTCGCCGCCTCGATCCCGCCCTTCCCGTTGACGATCTCTTGTCCACCATACAAACAGATGAAGGGGATCCTCCTCCTCCAGTAGCTGCCCCCTCCGATCAACCTTCCATTCGTCGAAGGTCTTCCGCTCCGGCTCCGGCGCCAGGGCCTTCGACAACTTCCTCGATATCATATAGTCAGGAACAAATTTCAATTATAAGAGAAATTAAGAGGAAGAAGAATTATTACGATATTTTAGGGTTGGAGAAGAGTTGCACTGTGGATGATGTCAGGAAATCTTATAGGAAGCTCTCTTTGAAGGTTCATCCTGACAAGAACAAGGCCCCTGGAGCCGAGGACGCATTTAAGTTGGTGTCCAAGGCTTTTCAGTGTCTTGGTAATGAGGAGAGTAAGAGAAAGTATGATGTTTCTGGAGAGGATGAAGTGGTTTACGAAAGGCGCACAGCTAGACCGGCTTCTGCCAGAGGTTTTAATGGTTACTATGATGCTGATGTTGATGCTGAAGAAATATTTAGGAATTTCTTCTTTGGAGGAATGGCGCCTGCTGCTAATTTCGGCGGGTTTAGTTTTGGAGGGCCGGGGATGGCTCATAGACAGCCAGCTGCTGATAATAGGTCTGGTGGATTCAATGTCCGAGCTTTGATTCAGGTGCTTCCTGTGCTTCTCATTTTGCTGATTAACTTTTTGCCTTCATCGGATCCTGTGTACACTCTTTCGCAAAACTATCCCTATGAACACCGGCTTACTACACCTAAGGGTGTTAACTACTATGTTAAGTCCACAAAGTTTGAACAAGATTATCCCTTGGATAGCCGCGAACGTCTTACAATTGAGGAAAGAGTTGAGAGAGAATACTTTGGTATTCTTCGCCAGAATTGTCAGTTTGAGATCCAGCGACGCCAGTGGGGATATATCAGGGAAACGCCTCACTGTGATATGCTAAGGAAGTATGATTCAGTCCGTTGATGAGTTTGAAACAAGGTTTAATTTTACTATTGTCTTGCTTTTGATGTAGATGCAGAGCTAAACTGCGTGTGATGATATTATAGAATGTACAACTTATTTTTTCACTCAGTAATTAGTGAAACAATTACTAATAAATCACTTTGATGGGATTGTTAGCATTAGCAATCAATTTTCATCTTAAAAGTCATTATTTATGTAAGATATATGATTGCAAACT
TARGET=435,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0093
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=CGATCTCTTGTCCACCATACAA
PRIMER_RIGHT_SEQUENCE=TGAACCTTCAAAGAGAGCTTCC
PRIMER_LEFT=398,22
PRIMER_RIGHT=670,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=273
PRIMER_PAIR_PENALTY_1=0.0537
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=0.046801
PRIMER_LEFT_1_SEQUENCE=CGATCTCTTGTCCACCATACAA
PRIMER_RIGHT_1_SEQUENCE=CACAGTGCAACTCTTCTCCAAC
PRIMER_LEFT_1=398,22
PRIMER_RIGHT_1=626,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=59.953
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=229
PRIMER_PAIR_PENALTY_2=0.0929
PRIMER_LEFT_2_PENALTY=0.006945
PRIMER_RIGHT_2_PENALTY=0.085951
PRIMER_LEFT_2_SEQUENCE=CGATCTCTTGTCCACCATACAA
PRIMER_RIGHT_2_SEQUENCE=CATCCACAGTGCAACTCTTCTC
PRIMER_LEFT_2=398,22
PRIMER_RIGHT_2=630,22
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.0929
PRIMER_LEFT_3_PENALTY=0.006945
PRIMER_RIGHT_3_PENALTY=0.085951
PRIMER_LEFT_3_SEQUENCE=CGATCTCTTGTCCACCATACAA
PRIMER_RIGHT_3_SEQUENCE=TCATCCACAGTGCAACTCTTCT
PRIMER_LEFT_3=398,22
PRIMER_RIGHT_3=631,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.1118
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.002310
PRIMER_LEFT_4_SEQUENCE=ACCATACAAACAGATGAAGGGG
PRIMER_RIGHT_4_SEQUENCE=TGAACCTTCAAAGAGAGCTTCC
PRIMER_LEFT_4=411,22
PRIMER_RIGHT_4=670,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.998
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=260
=
PRIMER_SEQUENCE_ID=SSR_04125 Ps_contig_mira-and-tgicl-ass_franssen_32200
SEQUENCE=CCTAAATACCCTTACAATAAAAAACAAGAAGGGGTTATAGCTGTTAGCCTATTACAGTACTTACACAAGCTCTGAATTCTCTATCTATATAGAGAATTAAAATCTCATATTAAAAATACAAAAAACCTGACTTTTTTTTGGGTTTAAAAACAAAATTTCATGGGTYTTCACGAAAGACAGCTGCCAAACCAGTCACAACCTTAACCCCGGTCAGCGAGTTTTTGGGTTTCAATTCTGGTGGCTTCCATTCAACCACCACTTTTGACTCTGACTCATCGCGACTCACTTCAGACTCACCACCACCACCACCAATCAAAACCCTCTGTTTTCTCTCAGTTTCCTCACTGATTTCACGAGGCCAATTGCAAAACAGAGGTTGTTTGGTTCTTGTGTAAGGAGATAACCATTTTGATCTAACGTACTCGAGTTTTCTCCATGGTGGAACATGCAAACCCTTCTTTTTCAAACTCTGTTTCGCTGCCTCAGAAGCTATGGCTACAATACTCTGCAAACGATCACTCTTCCCTACAAAAACATAAGGAAGCGTTTGGAGAATCGCTTTGTAAGACTTGGTTGATCGAGCAATCTCAAATTCTGATCTGAAATCAACATCAATTATCACCCTCTCCTTTCCCATTACAACATCAATATATTCATATTCCCCGGCGATACAAGAAAGTTGTACTTTTTCCCAGCGAGTATTTGACAAATTGAAGCATCGTATACCAAGAAGTTAAACAA
TARGET=295,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04126 Ps_contig_mira-and-tgicl-ass_franssen_32206
SEQUENCE=CTTGCATACATTTGGAGTTTGATTTATCAGGGACTGGTGTAACATACGAAACTGGAGACCACGTGGGTGTTTATGCTGAGAACTATGATGAAACTGTTGAAGAAGCTGGGAAGCTGTTGGGTCAGAATTTAGATTTGCTTTTTTCTCTTCACATCGACAATGAAGATGGCACTTCCCTAGGTGGTTCTCTTCTACCTCCTTTCCCTGGTCCTTGCACAGTGCGCACTGCATTAGCATGTTATGCAGATCTCTTGAACCCCCCACGAAAGGTTGCTTTAGTTGCATTAGCTGCTCATGCTTCTGAGCCTAGTGAAGCAGAAAGATTGAAGTTTCTCTCATCTCCTCAGGGAAAGGATGAATACTCCAAATGGGTTGTTGGAAGCCAGAGAAGTCTCCTTGAAGTAATGGCTGAATTTCCATCAGCAAAACCTCCCCTCGGTGTTTTTTTTGCTGCCATAGCCCCTCGTTTGCAGCCTCGTTATTATTCTATTTCATCCTCTCCTAGGCTTGCCCCACAAAGGGTACATGTAACTTGTGCTCTGGTAGAAGGTCCAACTCCGACTGGCAGAATTCACAAAGGAGTATGTTCAACTTGGATGAAGAATGCCATTCCCTTGGAGAAAAGTGGTGACTGTAGCCGGGCTCCCATATTTATCAGACCATCAAATTTCAAGCTACCAGCCGATCATTCAATTCCTATTATTATGGTTGGACCTGGTACTGGTTTTGCACCTTTTAGGGGATTTTTACAGGAGAGGCTTGCCCTCAAAGAGGACGGTGTTCAACTTGGTCCTGCATTACTATTCTTTGGGTGCAGGAACCGTCAAATGGATTTTATTTATGAGGATGAGCTAAATAATTTTGTGGAACAAGGTGCTCTGTCAGAGTTGATAGTTGCATTCTCTAGAGAGGGGCCTGAAAAGGAGTATGTTCAACACAAAATGATGGATAAAGCAGAATACTTGTGGAGTCTGATTTCTCAGGGTGGTTATCTTTATGTTTGTGGTGATGCCAAGGGCATGGCTAGAGATGTTCATCGAAGTCTTCATACCATTGTCCAGCAGCAGGAAAATGCGGACTCTTCAAAGGCAGAGGCTACAGTGAAAAAACTCCAGATGGATGGACGTTACCTAAGGGATGTCTGGTGATTTTCCTGATTGTTCATCTGTCTGGGTTGTGGTGTCCTTGGTATTAGATATGTTATTTTCAAGATGGTTGAAAGATATATTTTTTAGAGTAAGTACTGCTAGGAAATTTTGGGGCAGAGGGGGGAGGCTGAACAAGTTTCCTCGAGGAAAAAACTATTTTATGGGCATTTGCCTCTTTCTTTATTATCATCTTTATATTCTCTTATTGTGATAGGCTAGATCCCTTTGGACTAGGTTTATATAGGGGATTGTATAAATAATAATAATAATAGTGTAGAAATTGGTTACCAACTTCCCAATAATTATTTTATCAATGAAACTGGTTAAATTTTTTTAAAATANTNTGGATCATGGATGTTCAACAACT
TARGET=1404,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04127 Ps_contig_mira-and-tgicl-ass_franssen_32209
SEQUENCE=ACTTTTTTAAACTAAATTTTAAGAACTATTTTCTAATTTRAAAAGTATAAAACATTCTTTTTTCTATATAATTTTCTGCTCTCTCAAACAGGTCATAAATTGGTTTTCGTGCTGACCAATTGCAATCTATATTTTCACAATCAAATAAAATGGACCATTTTAAGTTCCTGCGACATAAACAACTAAGCATTCTACAAAGCATTTAACTCCAAGATAGACCAAGTGCTTGAGATCCTTACACATGGGTAGCCCAAAACACAACAATGTTACATAAACATAACCTAAAGAATAGCCACATCCAAGTAATCGCCAATCTCAAAACCAAGCTCAGCTAAAGCCTTGTTATCATCCAATCTGCCATTTCCAAAAGATAATGTCTTACCCACCTCTTGAACTTTGAAACGACCATTTTTATCAGGAAACACAAAAGAAAAGGAGAGTTTTGCATTTCTTCTTCTTGCAGCAGGAGCCACTTCTTTGACAAGATCGGTTAATTCTCGGAGTGTAGCATCTTTCCAGGTATAAATCTGAACTTCATCTTTAGGTTCTTTCCCTCTAACCGAAAAATCATCCATTGTATGATGACTTCCAATCTTAGTGAAAACACGCAAGAGTAAGGGACAAGTCTTTTCGCGATCGACGGGCTCGAGGCGAGGGCGAGAGGAGGGCGGAGGAGGACCTCTTGGCGGCGGCGGTAATGGTCTTCCGCCTTGTCGTTTTGCACCTCCGTCGCTCATTTCGTCGTTTTCTAGTTTGTGTTTTTTCTTTTTCTTCTTCTTCCTTTCCTCTATTCTCNGTTCTTCTTACC
TARGET=769,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04128 Ps_contig_mira-and-tgicl-ass_franssen_32217
SEQUENCE=GTCGGGGCTCTTCACTCTNNNGACGAGACCACTCATTACACACACCACTCACAACAAATTCATTCACTATCTCTCTCTCACCACTCAGACTCTCTCTATTAACCAACAAACAAAAACTTTTTCCAGGATTNATCTCTCTATCTCAGAAAATCTTCTTTATCTTTCTCCAGATTCTCTCTCACCATCAAAGCACCTCCGACCCACCTCCACAAAATCTCCACATGCGCCGCTAAACCACCCTTTGCCGTCAAACCCTTAAACTCACCCTCTTCACCGTTAACCCTCCGACGAATCCCAAAACCACAACCTCAAACGACCTCCGTTTCTTTTCCAATCCTTTCACAACCGTCGTCCTATTCACCGCACATCCGACCACTCACCGTGGCAGAATCTTGTCGGTGATAGCTAAACCTTCCATCATCAATACTGTTCACAACATCACAACACGGGTCCGTCACCGTCATCTGGGACAACGACACGCAAACGACCGTCAAAACCATGACCTCACGCATTCGCCGTTGATCTTCTTCCACCAAACCGAAAACAACTCAGTGGTGCCATAGGATATCAAACAAGGACGATGCCACTTGCTTCAATTTTGTTTTGGACAATTTCAAGTTAGAGGGTTTTTATTGATCGGTTCGCTGCTGGTTTTTGTTTGGTTGTTTATGCAGGTAAGGGTTCTGTTAAGACTCGGAACGGAAAGATCCAGATTCGCTTCATCAACGGTGCTGCATTCGAATGTGTTTCGGAATAAGTGTTGGATTTATGTCGACATTGCAGTAGTCCTGTAAACAATTAAGTTGTTCTTATTGTGGGCACCTTATCATGTTAGAAATTCTGCTGCTAAAAATTTTCATTTTATGAATGGTTCACATAAGGCTTCGGTTTATTAAGTATTTGTTGTGG
TARGET=70,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6331
PRIMER_LEFT_PENALTY=0.599919
PRIMER_RIGHT_PENALTY=0.033137
PRIMER_LEFT_SEQUENCE=NGACGAGACCACTCATTACACA
PRIMER_RIGHT_SEQUENCE=TAACGGTGAAGAGGGTGAGTTT
PRIMER_LEFT=20,22
PRIMER_RIGHT=278,22
PRIMER_LEFT_TM=59.400
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=0.6331
PRIMER_LEFT_1_PENALTY=0.599919
PRIMER_RIGHT_1_PENALTY=0.033137
PRIMER_LEFT_1_SEQUENCE=NGACGAGACCACTCATTACACA
PRIMER_RIGHT_1_SEQUENCE=TTAACGGTGAAGAGGGTGAGTT
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=279,22
PRIMER_LEFT_1_TM=59.400
PRIMER_RIGHT_1_TM=60.033
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=260
PRIMER_PAIR_PENALTY_2=0.6331
PRIMER_LEFT_2_PENALTY=0.599919
PRIMER_RIGHT_2_PENALTY=0.033137
PRIMER_LEFT_2_SEQUENCE=NGACGAGACCACTCATTACACA
PRIMER_RIGHT_2_SEQUENCE=AACGGTGAAGAGGGTGAGTTTA
PRIMER_LEFT_2=20,22
PRIMER_RIGHT_2=277,22
PRIMER_LEFT_2_TM=59.400
PRIMER_RIGHT_2_TM=60.033
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.6479
PRIMER_LEFT_3_PENALTY=0.490462
PRIMER_RIGHT_3_PENALTY=0.157415
PRIMER_LEFT_3_SEQUENCE=CACACCACTCACAACAAATTCA
PRIMER_RIGHT_3_SEQUENCE=CGGTGAAGAGGGTGAGTTTAAG
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=275,22
PRIMER_LEFT_3_TM=59.510
PRIMER_RIGHT_3_TM=60.157
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.6874
PRIMER_LEFT_4_PENALTY=0.490462
PRIMER_RIGHT_4_PENALTY=0.196903
PRIMER_LEFT_4_SEQUENCE=CACACCACTCACAACAAATTCA
PRIMER_RIGHT_4_SEQUENCE=TGATGGTGAGAGAGAATCTGGA
PRIMER_LEFT_4=40,22
PRIMER_RIGHT_4=186,22
PRIMER_LEFT_4_TM=59.510
PRIMER_RIGHT_4_TM=59.803
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_04129 Ps_contig_mira-and-tgicl-ass_franssen_32219
SEQUENCE=CGGAAGGAGAAAAGAGAAAAGAAAAGAATGAATATGTTGGTGGTGTTCCTCAACTGAAGCAAAAAAAGTGTAGTCGTTTACTTCTTTTCATCTTCTTCTCTCTCTTTAGTCCCAATACCACATAAATGAACTCATACATACATTTCCATATTACATATACATACACTGTACTCTCTCTCTCCCTCTCTCTCCGGTACCTTTCCACTGTACTTTTTTGAGAAAGCCACGCCACATCAACATTTAGCGTAAATACCTACAAAGAATGAGTTCATCCTCAGGCCAGACTACTACTGGCCATGATCTCTCTTTCAAGATCTTACTCATCGGTGATTCTGGTGTTGGAAAAAGTTGCCTACTCGTTACCTTCATTTCCAGTTCATCTATCGAAGATCTTTCACCAACCATTGGTGTTGATTTTAAGATCAAAACACTCACTGTAGGTGGGAAGAGATTAAAACTTACAATTTGGGACACAGCTGGGCAGGAAAGGTTCAGGACTCTAACAAGTTCTTACTATAGAGGAGCGCAAGGAATTATTCTCGTTTATGATGTCACGAAGAGAGAAACCTTTACAAATTTATCTGAAGTGTGGTCTAAGGAATTGGAACTCTATTCAACTAACCAGGAGTGCGTGAAGATGCTAGTTGGAAATAAAGTTGACAGAGATTCTGAAAGGGCTGTGAGCAAGGAAGAGGGATTAGCCCTTGCTAAAGAATTGGGGTGTTTGCTTCTTGAATGTAGTGCTAAAACTCGAGAGAATGTGGAGAAGTGCTTTGAAGAACTCGCTCTAAAGATAATGGAGGCGCCTAGTCTATTGGAAGAAGGATCAACAGCAATAAAAAGAAAATGTTTTAAAACAGAAACAAGAACCACAAGCACCACGGAACGGCGGCTGTTGCTCTTAAAATGCTGCATTGTGAACATTTTGGAAAATCCTGTATATGTGACTGTGAGCTTTTTCCCTAGTTTTTTTACTGCTGCCAGTGTTCTTTTAGTTGCTCAATTACTTGTGAGTTGGGGGAAATTGGCAACAATGACTATTTTATCAGCTTGTTAGTTAAATTTTGTTTGTCCTTTGAATGTATTCTATCTATTTGTGTGCTGCCTCCCAAGTAC
TARGET=171,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2696
PRIMER_LEFT_PENALTY=0.242742
PRIMER_RIGHT_PENALTY=0.026872
PRIMER_LEFT_SEQUENCE=TATGTTGGTGGTGTTCCTCAAC
PRIMER_RIGHT_SEQUENCE=TTACGCTAAATGTTGATGTGGC
PRIMER_LEFT=32,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=59.757
PRIMER_RIGHT_TM=60.027
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.3208
PRIMER_LEFT_1_PENALTY=0.242742
PRIMER_RIGHT_1_PENALTY=0.078029
PRIMER_LEFT_1_SEQUENCE=TATGTTGGTGGTGTTCCTCAAC
PRIMER_RIGHT_1_SEQUENCE=GTACAGTGGAAAGGTACCGGAG
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=209,22
PRIMER_LEFT_1_TM=59.757
PRIMER_RIGHT_1_TM=59.922
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.3208
PRIMER_LEFT_2_PENALTY=0.242742
PRIMER_RIGHT_2_PENALTY=0.078029
PRIMER_LEFT_2_SEQUENCE=TATGTTGGTGGTGTTCCTCAAC
PRIMER_RIGHT_2_SEQUENCE=AGTACAGTGGAAAGGTACCGGA
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=210,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=59.922
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.5106
PRIMER_LEFT_3_PENALTY=0.242742
PRIMER_RIGHT_3_PENALTY=0.267873
PRIMER_LEFT_3_SEQUENCE=TATGTTGGTGGTGTTCCTCAAC
PRIMER_RIGHT_3_SEQUENCE=AGTAGTCTGGCCTGAGGATGAA
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=288,22
PRIMER_LEFT_3_TM=59.757
PRIMER_RIGHT_3_TM=60.268
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.5106
PRIMER_LEFT_4_PENALTY=0.242742
PRIMER_RIGHT_4_PENALTY=0.267873
PRIMER_LEFT_4_SEQUENCE=TATGTTGGTGGTGTTCCTCAAC
PRIMER_RIGHT_4_SEQUENCE=TAGTCTGGCCTGAGGATGAACT
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=286,22
PRIMER_LEFT_4_TM=59.757
PRIMER_RIGHT_4_TM=60.268
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_04130 Ps_contig_mira-and-tgicl-ass_franssen_32223
SEQUENCE=CTTAAACCAATAATATCANAGAAAAGAAAAACAAGCCTCAATTTTACTAAAATACATAACACGAAATTAAATTTGCTTAAATTAATTCAACTTGTTCAAAGTCTTGGCAATATTATTGTAAGTGTTTTGTTTTGTTTGGAGTATACATTTTCACTGCTCAAGTTTACTTCCTTTCACATCCACAACAAACCTATACCTCACATCATTCTTCTCCAATCTTTCAAAAGCTTTGTTAATGTAATCCATAGTCACAACTTCAATCATGGAGGTTAAACCCTTCTCCTTCCAAAACTCCAACATCTCCTCCGTCTCCTTCACGCTCCCAACAAAGCTTCCGGTAATCGTCTTCCTCCCTAGCATGACCATAGGTGTGACAAATTGAAGAGGGGTGTTAATAACACCCATCAAGATCAATTTTCCATCAACTTTAAGCAATGCAAGATAAGGCTCAAGAGGGTGACCAACAGGAACAGTATCAATAATATAATCAAGTGAATCAGCAATTTCTTGCATGGCACTTGTGTCTGAGCTAACAACATAATTGTCAGCTCCAAGATCCTCAATTGCTTCTTTCTTCTTCTTATCGGAAGAACTTATCACAGTCACATGGTGACCGAATGCTTTTGCTACTTTCACCCCCATGTGTCCTACACCTCCCAGGCCCAATATTCCACCTCTTAGCCCACTTTGTTTCAGCCCAAAATGTGCAAGTGGACTGTACACTGTCACACCAGCACATAACAATGGTGCTGCTTGCTCTGGTGCCAATCCCTCTGGAACTTTCACAACGAACTTCTGTTCGACGACGGTGGTTTCGGCGAAACCACCCTGAGTGATTTTTCCGTCTGTATAAACATCATTGTAAGACCAGATCTTCTTGTTACAATATTGTTCAATATCCGAATCACAAGCCCTGCAGTTTTTGCAGCAACCAACAAGAAGTCCAACTCCCACAATTTCTCCTACACTGAATCTGGTGACATTTGATCCCACCTCTACGTACCTCACCAACCACTTCATGCCCGGGAACCATGGGATAATTGGACATGCCAAGATCATTTTTGATTTGGTGAAGATCAGAATGACAAACTCCACAGTAATAAATTTTGATGTAGACATCATCTGCTCCTGTGTTTCTAAGAGTGTAAGTATAGGGAGTTAGAATTCCGGAAGGGTCGGTTGCAGCCAATCCAACTGTTGTCCTTTCAGCAGCTTCAAGGCTACCCATTCTTGAGAACAAAAAGATGAAAAAGTGAGAGAGAGAGAGAGGAAATAGTGCAGAGTGTATGTCGGTTTCCCAAC
TARGET=123,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6069
PRIMER_LEFT_PENALTY=0.598673
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=TCAACTTGTTCAAAGTCTTGGC
PRIMER_RIGHT_SEQUENCE=TTGTTGTGGATGTGAAAGGAAG
PRIMER_LEFT=86,22
PRIMER_RIGHT=187,22
PRIMER_LEFT_TM=59.401
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=0.6139
PRIMER_LEFT_1_PENALTY=0.598673
PRIMER_RIGHT_1_PENALTY=0.015253
PRIMER_LEFT_1_SEQUENCE=TCAACTTGTTCAAAGTCTTGGC
PRIMER_RIGHT_1_SEQUENCE=TCACACCTATGGTCATGCTAGG
PRIMER_LEFT_1=86,22
PRIMER_RIGHT_1=373,22
PRIMER_LEFT_1_TM=59.401
PRIMER_RIGHT_1_TM=60.015
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.6170
PRIMER_LEFT_2_PENALTY=0.598673
PRIMER_RIGHT_2_PENALTY=0.018372
PRIMER_LEFT_2_SEQUENCE=TCAACTTGTTCAAAGTCTTGGC
PRIMER_RIGHT_2_SEQUENCE=GAGTTTTGGAAGGAGAAGGGTT
PRIMER_LEFT_2=86,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=59.401
PRIMER_RIGHT_2_TM=59.982
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.6222
PRIMER_LEFT_3_PENALTY=0.598673
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=TCAACTTGTTCAAAGTCTTGGC
PRIMER_RIGHT_3_SEQUENCE=ATGTTGGAGTTTTGGAAGGAGA
PRIMER_LEFT_3=86,22
PRIMER_RIGHT_3=299,22
PRIMER_LEFT_3_TM=59.401
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.6222
PRIMER_LEFT_4_PENALTY=0.598673
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=TCAACTTGTTCAAAGTCTTGGC
PRIMER_RIGHT_4_SEQUENCE=GATGTTGGAGTTTTGGAAGGAG
PRIMER_LEFT_4=86,22
PRIMER_RIGHT_4=300,22
PRIMER_LEFT_4_TM=59.401
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_04131 Ps_contig_mira-and-tgicl-ass_franssen_32223
SEQUENCE=CTTAAACCAATAATATCANAGAAAAGAAAAACAAGCCTCAATTTTACTAAAATACATAACACGAAATTAAATTTGCTTAAATTAATTCAACTTGTTCAAAGTCTTGGCAATATTATTGTAAGTGTTTTGTTTTGTTTGGAGTATACATTTTCACTGCTCAAGTTTACTTCCTTTCACATCCACAACAAACCTATACCTCACATCATTCTTCTCCAATCTTTCAAAAGCTTTGTTAATGTAATCCATAGTCACAACTTCAATCATGGAGGTTAAACCCTTCTCCTTCCAAAACTCCAACATCTCCTCCGTCTCCTTCACGCTCCCAACAAAGCTTCCGGTAATCGTCTTCCTCCCTAGCATGACCATAGGTGTGACAAATTGAAGAGGGGTGTTAATAACACCCATCAAGATCAATTTTCCATCAACTTTAAGCAATGCAAGATAAGGCTCAAGAGGGTGACCAACAGGAACAGTATCAATAATATAATCAAGTGAATCAGCAATTTCTTGCATGGCACTTGTGTCTGAGCTAACAACATAATTGTCAGCTCCAAGATCCTCAATTGCTTCTTTCTTCTTCTTATCGGAAGAACTTATCACAGTCACATGGTGACCGAATGCTTTTGCTACTTTCACCCCCATGTGTCCTACACCTCCCAGGCCCAATATTCCACCTCTTAGCCCACTTTGTTTCAGCCCAAAATGTGCAAGTGGACTGTACACTGTCACACCAGCACATAACAATGGTGCTGCTTGCTCTGGTGCCAATCCCTCTGGAACTTTCACAACGAACTTCTGTTCGACGACGGTGGTTTCGGCGAAACCACCCTGAGTGATTTTTCCGTCTGTATAAACATCATTGTAAGACCAGATCTTCTTGTTACAATATTGTTCAATATCCGAATCACAAGCCCTGCAGTTTTTGCAGCAACCAACAAGAAGTCCAACTCCCACAATTTCTCCTACACTGAATCTGGTGACATTTGATCCCACCTCTACGTACCTCACCAACCACTTCATGCCCGGGAACCATGGGATAATTGGACATGCCAAGATCATTTTTGATTTGGTGAAGATCAGAATGACAAACTCCACAGTAATAAATTTTGATGTAGACATCATCTGCTCCTGTGTTTCTAAGAGTGTAAGTATAGGGAGTTAGAATTCCGGAAGGGTCGGTTGCAGCCAATCCAACTGTTGTCCTTTCAGCAGCTTCAAGGCTACCCATTCTTGAGAACAAAAAGATGAAAAAGTGAGAGAGAGAGAGAGGAAATAGTGCAGAGTGTATGTCGGTTTCCCAAC
TARGET=1255,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5492
PRIMER_LEFT_PENALTY=0.216941
PRIMER_RIGHT_PENALTY=0.332305
PRIMER_LEFT_SEQUENCE=GAACCATGGGATAATTGGACAT
PRIMER_RIGHT_SEQUENCE=GAAACCGACATACACTCTGCAC
PRIMER_LEFT=1026,22
PRIMER_RIGHT=1296,22
PRIMER_LEFT_TM=59.783
PRIMER_RIGHT_TM=59.668
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.6416
PRIMER_LEFT_1_PENALTY=0.309343
PRIMER_RIGHT_1_PENALTY=0.332305
PRIMER_LEFT_1_SEQUENCE=GAGTTAGAATTCCGGAAGGGTC
PRIMER_RIGHT_1_SEQUENCE=GAAACCGACATACACTCTGCAC
PRIMER_LEFT_1=1155,22
PRIMER_RIGHT_1=1296,22
PRIMER_LEFT_1_TM=60.309
PRIMER_RIGHT_1_TM=59.668
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=142
PRIMER_PAIR_PENALTY_2=0.8087
PRIMER_LEFT_2_PENALTY=0.476376
PRIMER_RIGHT_2_PENALTY=0.332305
PRIMER_LEFT_2_SEQUENCE=ACTGTTGTCCTTTCAGCAGCTT
PRIMER_RIGHT_2_SEQUENCE=GAAACCGACATACACTCTGCAC
PRIMER_LEFT_2=1193,22
PRIMER_RIGHT_2=1296,22
PRIMER_LEFT_2_TM=60.476
PRIMER_RIGHT_2_TM=59.668
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=0.8670
PRIMER_LEFT_3_PENALTY=0.216941
PRIMER_RIGHT_3_PENALTY=0.650009
PRIMER_LEFT_3_SEQUENCE=GAACCATGGGATAATTGGACAT
PRIMER_RIGHT_3_SEQUENCE=GTTGGGAAACCGACATACACTC
PRIMER_LEFT_3=1026,22
PRIMER_RIGHT_3=1301,22
PRIMER_LEFT_3_TM=59.783
PRIMER_RIGHT_3_TM=60.650
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.9594
PRIMER_LEFT_4_PENALTY=0.309343
PRIMER_RIGHT_4_PENALTY=0.650009
PRIMER_LEFT_4_SEQUENCE=GAGTTAGAATTCCGGAAGGGTC
PRIMER_RIGHT_4_SEQUENCE=GTTGGGAAACCGACATACACTC
PRIMER_LEFT_4=1155,22
PRIMER_RIGHT_4=1301,22
PRIMER_LEFT_4_TM=60.309
PRIMER_RIGHT_4_TM=60.650
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_04132 Ps_contig_mira-and-tgicl-ass_franssen_32224
SEQUENCE=CAACGCAGAGAGCGGGAAGGAGAAACGAAGAAAATNACGCGGTGTGTTCTTCGTAAGGTTTTAGTACGCATATCACCAAAATCGAATAATNCTTCATAATATATTGCTTCACTAGGATCTAACCTTAAATAATTTATTTTCAAGCAATAGTTTTTGTGGGTAACTCTCAATTTTTCCGATCCCCAATTTTTCTTTGTATATATATTTTTGGGTTCTGGGATCAGTGTGAAATGGAGAAGGTGATGAACATTCTGAAACCCAAGCCAAATCCTCAACAAATATTGAGAGATTGGCAACGCAGGCTTCGCCAAGAGTGTCGAAACATTGAGCGTCAAATTCGCGATATTGAGAGAGAAGAAAAAACTGTGCAGAAGGCTATTAAAGAAGCTGCCAAGAGGAATGACATTGGCTCTGCAAAGGCACTTGCTACGGAAATTGTGAGATCGAGGAAAACAGTGAACCGGCTTCATGAAAATAAAGCGCAATTGAATTCAATATCAATGCACCTTGGTGAAAGTGTTGCGATTTCTCGCACTGTGGGACATCTATCCAAGAGTGCTGAGGTTATGAAGCTTGTCAATAACCTCATGAAGGCTCCTGAAATGGCTGTGGCTATGCAAGAGTTCAGCAAAGAAATGACAAAGGCAGGAGTCATTGAAGAGATAGTAAACGATGCTGTTGACTCGGCATTAGATTCTGAGGACATAGAAGATGAGATAGAAGAAGAAGTTGACAAAGTCTTAACCGCAATAGCTGGCGAGACAGCCGCACAACTTCCTGAAGCTGCGAGGAAACAAAAGGTGAAGCAACCTGGTCAAAGTGTTAGTGCTGCAGAGGAAGAGGCTATAGCTGAGGGTGTTGATGATGAAGAGGAAATGGAGGAAATTAGGGCCAGATTGGCTAAAGTCAGGTCATAAACGAGTTTATGCTCCAGATTGTACCTTGATATATATATTTTAAGAGAGATGTTGACATCTTTGCATCCCATTTGTATTGGAAAATATGACTAATATATATCACCAATGTCAACATAGTTCATATGTAAATTATAAACAATTTGAAGCTTCAAGTTTATATTATTGAGGACAATGCTAACTTTATCCCATGGTTGAGGA
TARGET=946,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3930
PRIMER_LEFT_PENALTY=0.096820
PRIMER_RIGHT_PENALTY=0.296177
PRIMER_LEFT_SEQUENCE=GATGATGAAGAGGAAATGGAGG
PRIMER_RIGHT_SEQUENCE=TCCTCAACCATGGGATAAAGTT
PRIMER_LEFT=860,22
PRIMER_RIGHT=1114,22
PRIMER_LEFT_TM=59.903
PRIMER_RIGHT_TM=59.704
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=255
PRIMER_PAIR_PENALTY_1=0.3930
PRIMER_LEFT_1_PENALTY=0.096820
PRIMER_RIGHT_1_PENALTY=0.296177
PRIMER_LEFT_1_SEQUENCE=ATGATGAAGAGGAAATGGAGGA
PRIMER_RIGHT_1_SEQUENCE=TCCTCAACCATGGGATAAAGTT
PRIMER_LEFT_1=861,22
PRIMER_RIGHT_1=1114,22
PRIMER_LEFT_1_TM=59.903
PRIMER_RIGHT_1_TM=59.704
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=254
PRIMER_PAIR_PENALTY_2=0.4062
PRIMER_LEFT_2_PENALTY=0.110004
PRIMER_RIGHT_2_PENALTY=0.296177
PRIMER_LEFT_2_SEQUENCE=CAAAGTGTTAGTGCTGCAGAGG
PRIMER_RIGHT_2_SEQUENCE=TCCTCAACCATGGGATAAAGTT
PRIMER_LEFT_2=815,22
PRIMER_RIGHT_2=1114,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.704
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.4073
PRIMER_LEFT_3_PENALTY=0.111077
PRIMER_RIGHT_3_PENALTY=0.296177
PRIMER_LEFT_3_SEQUENCE=TGAGGGTGTTGATGATGAAGAG
PRIMER_RIGHT_3_SEQUENCE=TCCTCAACCATGGGATAAAGTT
PRIMER_LEFT_3=850,22
PRIMER_RIGHT_3=1114,22
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=59.704
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=265
PRIMER_PAIR_PENALTY_4=0.4265
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.296177
PRIMER_LEFT_4_SEQUENCE=CCAGATTGGCTAAAGTCAGGTC
PRIMER_RIGHT_4_SEQUENCE=TCCTCAACCATGGGATAAAGTT
PRIMER_LEFT_4=891,22
PRIMER_RIGHT_4=1114,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.704
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_04133 Ps_contig_mira-and-tgicl-ass_franssen_32227
SEQUENCE=AAAGGGGTTTTTTTTAAAATTTTTACTTCCATTCAATCTAAATACTGTATTATATATATTGAACATATAATGACTTAATGATTCTATAAGGTAGTATATTTTATCTTTACAAGATGAAAACATAAACTAGAATGGTTTTTTTTTAAGTTAAAGCCAACTGGTAGAATACACATTCCAACTTGGCAGGAGTTAAGCCATTTGTACTATACTACTCTTATGATCAATCAAACCCTTAATGCCACTTGGTCTTGCCATGTGATCTTCCTTTTCCCTGCTCTACTTGATCCTTCTCCAAGGTTTCTACAAACTGAAAGCTTGATATCATTGTGCTGTTCCTCATTCACACCTCTTTGTGTACTACTCTTCTTTTGTTCTTCAATCCACATTGGCTGATTGTTTCCCTCTAATTCCTCCTCTTCTCTCGAAATCTGGTTCAAGTCGAAAAATGGTGTTACACTTTTCTTTGTGGCTTCTTTCCCACTATGAACCCTTTCTTTGTGATTCAAGTCAAGCACAGGAGCAGAAGAGCTATGAAAAGAACCATCTTTTTTACTTAAGCTTCTACCATTCTGGTTTAAATCAAACACATGGCTAGTTTTCTGCCTTGTGTCTTCAACAAATCCATTGAAAACTCTTTCTTTTGAGTTCGATTTTGAACCGGTGTGAGGTCTCAGGGTAGATTCCTTTCTATTGTAGCTCCTTCCCTTTGTGATCTTTGGCTCTTTGATTTTCAGCTTTTGTGGCTTTATAACTTCTTGCTTTGGTTGTGCCTTAGGAAGAGGATGTTTTAACAAGTATGTGTAACGGTGCCTTAAGAATCTAACTTCAGCTGAAAGAATCAACTTTTTCTGCTTCACAGCTTCCAATTTCATTCTCATGGCCTCTGTTTCCTTATGCAAATCTTCATAGTCCTGCAAGAGACTCCGGTGCCTCATGATAGATCTCTGATCCATAAACACATCAGACGAAGGATTGATGGAAACGGCAACAGCTTTCATCTTCTTCATCATGAGGAGATGAGAGAAGAAAAGAGAAACAAAACTCTAGAGAGAGAAAGAATAAATAAGAAAGGTGACAGAGGAAGAGAGATATAGATATAGTGAGAGAGAGAATAGAGAAGAGAGAAAAAGAAGAGGCGAAATAATAGTAGAAATAATAAAGGAAGAAAGAAGGGGAAAGGGTTAAGAAGGTTCTCGTATTTCTGTGAAACTCTGACCAAGAGAAAGAAACCGAAAAAGCCCACATAACTCGGTGAAAAATATTCTTGTTTTGAAAAACCAGTGATGAAGATGGGATTTTTTGGGGGCTTGCAGAAGGGTAATTTCGGGAACATGTTTTCATGAGAGAGAGAGAGAGTAATAAGGTGGGTTTGGTTGGAGGACAATTGAACACATCTAATCTATAACATAACAAGACCTCTATCTTTTGTTTCCC
TARGET=1102,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4717
PRIMER_LEFT_PENALTY=0.362762
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=GCAACAGCTTTCATCTTCTTCA
PRIMER_RIGHT_SEQUENCE=ATTTTTCACCGAGTTATGTGGG
PRIMER_LEFT=984,22
PRIMER_RIGHT=1259,22
PRIMER_LEFT_TM=59.637
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.4895
PRIMER_LEFT_1_PENALTY=0.380537
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=GGCAACAGCTTTCATCTTCTTC
PRIMER_RIGHT_1_SEQUENCE=ATTTTTCACCGAGTTATGTGGG
PRIMER_LEFT_1=983,22
PRIMER_RIGHT_1=1259,22
PRIMER_LEFT_1_TM=60.381
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=277
PRIMER_PAIR_PENALTY_2=0.5932
PRIMER_LEFT_2_PENALTY=0.362762
PRIMER_RIGHT_2_PENALTY=0.230415
PRIMER_LEFT_2_SEQUENCE=GCAACAGCTTTCATCTTCTTCA
PRIMER_RIGHT_2_SEQUENCE=GGCTTTTTCGGTTTCTTTCTCT
PRIMER_LEFT_2=984,22
PRIMER_RIGHT_2=1239,22
PRIMER_LEFT_2_TM=59.637
PRIMER_RIGHT_2_TM=60.230
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.6110
PRIMER_LEFT_3_PENALTY=0.380537
PRIMER_RIGHT_3_PENALTY=0.230415
PRIMER_LEFT_3_SEQUENCE=GGCAACAGCTTTCATCTTCTTC
PRIMER_RIGHT_3_SEQUENCE=GGCTTTTTCGGTTTCTTTCTCT
PRIMER_LEFT_3=983,22
PRIMER_RIGHT_3=1239,22
PRIMER_LEFT_3_TM=60.381
PRIMER_RIGHT_3_TM=60.230
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.6217
PRIMER_LEFT_4_PENALTY=0.362762
PRIMER_RIGHT_4_PENALTY=0.258908
PRIMER_LEFT_4_SEQUENCE=GCAACAGCTTTCATCTTCTTCA
PRIMER_RIGHT_4_SEQUENCE=TTTCGGTTTCTTTCTCTTGGTC
PRIMER_LEFT_4=984,22
PRIMER_RIGHT_4=1234,22
PRIMER_LEFT_4_TM=59.637
PRIMER_RIGHT_4_TM=59.741
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_04134 Ps_contig_mira-and-tgicl-ass_franssen_32227
SEQUENCE=AAAGGGGTTTTTTTTAAAATTTTTACTTCCATTCAATCTAAATACTGTATTATATATATTGAACATATAATGACTTAATGATTCTATAAGGTAGTATATTTTATCTTTACAAGATGAAAACATAAACTAGAATGGTTTTTTTTTAAGTTAAAGCCAACTGGTAGAATACACATTCCAACTTGGCAGGAGTTAAGCCATTTGTACTATACTACTCTTATGATCAATCAAACCCTTAATGCCACTTGGTCTTGCCATGTGATCTTCCTTTTCCCTGCTCTACTTGATCCTTCTCCAAGGTTTCTACAAACTGAAAGCTTGATATCATTGTGCTGTTCCTCATTCACACCTCTTTGTGTACTACTCTTCTTTTGTTCTTCAATCCACATTGGCTGATTGTTTCCCTCTAATTCCTCCTCTTCTCTCGAAATCTGGTTCAAGTCGAAAAATGGTGTTACACTTTTCTTTGTGGCTTCTTTCCCACTATGAACCCTTTCTTTGTGATTCAAGTCAAGCACAGGAGCAGAAGAGCTATGAAAAGAACCATCTTTTTTACTTAAGCTTCTACCATTCTGGTTTAAATCAAACACATGGCTAGTTTTCTGCCTTGTGTCTTCAACAAATCCATTGAAAACTCTTTCTTTTGAGTTCGATTTTGAACCGGTGTGAGGTCTCAGGGTAGATTCCTTTCTATTGTAGCTCCTTCCCTTTGTGATCTTTGGCTCTTTGATTTTCAGCTTTTGTGGCTTTATAACTTCTTGCTTTGGTTGTGCCTTAGGAAGAGGATGTTTTAACAAGTATGTGTAACGGTGCCTTAAGAATCTAACTTCAGCTGAAAGAATCAACTTTTTCTGCTTCACAGCTTCCAATTTCATTCTCATGGCCTCTGTTTCCTTATGCAAATCTTCATAGTCCTGCAAGAGACTCCGGTGCCTCATGATAGATCTCTGATCCATAAACACATCAGACGAAGGATTGATGGAAACGGCAACAGCTTTCATCTTCTTCATCATGAGGAGATGAGAGAAGAAAAGAGAAACAAAACTCTAGAGAGAGAAAGAATAAATAAGAAAGGTGACAGAGGAAGAGAGATATAGATATAGTGAGAGAGAGAATAGAGAAGAGAGAAAAAGAAGAGGCGAAATAATAGTAGAAATAATAAAGGAAGAAAGAAGGGGAAAGGGTTAAGAAGGTTCTCGTATTTCTGTGAAACTCTGACCAAGAGAAAGAAACCGAAAAAGCCCACATAACTCGGTGAAAAATATTCTTGTTTTGAAAAACCAGTGATGAAGATGGGATTTTTTGGGGGCTTGCAGAAGGGTAATTTCGGGAACATGTTTTCATGAGAGAGAGAGAGAGTAATAAGGTGGGTTTGGTTGGAGGACAATTGAACACATCTAATCTATAACATAACAAGACCTCTATCTTTTGTTTCCC
TARGET=1342,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3506
PRIMER_LEFT_PENALTY=0.186949
PRIMER_RIGHT_PENALTY=0.163658
PRIMER_LEFT_SEQUENCE=CCAGTGATGAAGATGGGATTTT
PRIMER_RIGHT_SEQUENCE=GATGTGTTCAATTGTCCTCCAA
PRIMER_LEFT=1277,22
PRIMER_RIGHT=1394,22
PRIMER_LEFT_TM=60.187
PRIMER_RIGHT_TM=59.836
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=118
PRIMER_PAIR_PENALTY_1=0.3847
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=0.163658
PRIMER_LEFT_1_SEQUENCE=AAAAACCAGTGATGAAGATGGG
PRIMER_RIGHT_1_SEQUENCE=GATGTGTTCAATTGTCCTCCAA
PRIMER_LEFT_1=1272,22
PRIMER_RIGHT_1=1394,22
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=59.836
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=123
PRIMER_PAIR_PENALTY_2=0.3941
PRIMER_LEFT_2_PENALTY=0.230415
PRIMER_RIGHT_2_PENALTY=0.163658
PRIMER_LEFT_2_SEQUENCE=AGAGAAAGAAACCGAAAAAGCC
PRIMER_RIGHT_2_SEQUENCE=GATGTGTTCAATTGTCCTCCAA
PRIMER_LEFT_2=1218,22
PRIMER_RIGHT_2=1394,22
PRIMER_LEFT_2_TM=60.230
PRIMER_RIGHT_2_TM=59.836
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=0.4226
PRIMER_LEFT_3_PENALTY=0.258908
PRIMER_RIGHT_3_PENALTY=0.163658
PRIMER_LEFT_3_SEQUENCE=GACCAAGAGAAAGAAACCGAAA
PRIMER_RIGHT_3_SEQUENCE=GATGTGTTCAATTGTCCTCCAA
PRIMER_LEFT_3=1213,22
PRIMER_RIGHT_3=1394,22
PRIMER_LEFT_3_TM=59.741
PRIMER_RIGHT_3_TM=59.836
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=0.4675
PRIMER_LEFT_4_PENALTY=0.303792
PRIMER_RIGHT_4_PENALTY=0.163658
PRIMER_LEFT_4_SEQUENCE=ACCAGTGATGAAGATGGGATTT
PRIMER_RIGHT_4_SEQUENCE=GATGTGTTCAATTGTCCTCCAA
PRIMER_LEFT_4=1276,22
PRIMER_RIGHT_4=1394,22
PRIMER_LEFT_4_TM=59.696
PRIMER_RIGHT_4_TM=59.836
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_04135 Ps_contig_mira-and-tgicl-ass_franssen_32231
SEQUENCE=GGAAAGATTTTTTGAATATTTCTTTCTCCAAAAGTGTTACTTTAGAAGTTCATCAAAGATAGTTCTATATATATCCACAATAAAACCATCCAGTAATAATTGTGAACCACCAATACTAAGGTAAACCACTAAACTAATCAGCTACAACAACACATAGATAACTATCCGAAAAACCATAGGGCAGTAAAAGAAAAGAGACAAAAACTCATAAGAACCTCTAAGTAGAACCTTCTTCTCAACTCCTAGGACCAGCTCCTTCCCTCTCGAATTTCTGCGTGAAAAAATTGGTGCCATACATGTTATCGTCGAAATACTCTTTATAAGGATGATTCTTGGGAATAAGCCTTCGAAACTGATCGAATTGCTTCTCAGCTTCATCTTTCTTTCTAAGCAAGGTATAAATAATACCCTGGCATAGATAAGGCCTGAAGTCTCTCGGTTCTTCTTTTACCAGCTCCTGATAGGCTTTAAGAGCTTCAGAGAAATTCCCTTCCATCAGCTTAATCTGAGCAACCAATAACCTAAAGTCTCTAACATCTGAAACCCTATTCTGTTTCTTGCACGATTCCAATGCTTTTTCAACCCTTTCCAACAATTCTTTCATATGTTCATTAGACTCATTAGACTCATTAGACTCCGACGACGCCATAACAAGACCGTGAAAAGCTTCAACTCGAAACGGATCTCTCTGCAGAATCTCTTCGAATAATTTCTTAGCAGTTTCATGGTCATCGTTGTAGATATACATGTTAGCCTTCAATAGACGCCACTCCGTTTCTTCAGGCTCAATCTCAATCAAACGGTCGATGATATTAATGGCTTCATCAACCTTTCGTGCTTTGATTTTGACTTCCATGAGGTCACGAAGAGCTTCCGCGTCGTTTGGATTTTCACTCAACCGCTCTTCGATTAGCTTCTCCGAATCTTCTTCTGAAGCGTTTTCGTTTTCAGCTGAGGTATCAACGGTTGCCGGAGTAGCGAGCGTGGCGGCAATGACGGGAGTGTGATGGAAACGCATGAAGAAGAAGGCGGTGGCGGCGATGATGATGCAGGTGGTTTCAACGACGGGGGAGAAGAGAGAGGTTAGCGCTTGGAATGGGTTTGGTAAGGAAGGGAGGTTTCTTCTCGGTTGGTGAAACGCAGGGTCGTTTGAAGAGGATGAGGCTTTGATAGGTTGGAACTTAAACGACGGCGGTGGTGGGAGGGACATGAAGGAGAGAGAAGGTGATAACGGAGTTGGGAATGATGAACGGTTGTGATGGAGTGAGAGGTTGGAGTGACGGAGGTTAGTAACGGAATGAGAATTCATGATGAGTGTGGAGAAGTAGAATAGAGCGAAGCGAGGAAGAGAAGAGAAGAGAAGAGAAACCTAAAGTCTAAAACCCTCACTCTCTGAATCATTGATTAGTTTGGAACAGAAAGGGTAT
TARGET=1345,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7184
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.716126
PRIMER_LEFT_SEQUENCE=GAGAAGGTGATAACGGAGTTGG
PRIMER_RIGHT_SEQUENCE=TCAATGATTCAGAGAGTGAGGG
PRIMER_LEFT=1218,22
PRIMER_RIGHT=1403,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.284
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=186
PRIMER_PAIR_PENALTY_1=0.7308
PRIMER_LEFT_1_PENALTY=0.014720
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=CGGAGGTTAGTAACGGAATGAG
PRIMER_RIGHT_1_SEQUENCE=TCAATGATTCAGAGAGTGAGGG
PRIMER_LEFT_1=1280,22
PRIMER_RIGHT_1=1403,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.7350
PRIMER_LEFT_2_PENALTY=0.018835
PRIMER_RIGHT_2_PENALTY=0.716126
PRIMER_LEFT_2_SEQUENCE=GTTTCTTCTCGGTTGGTGAAAC
PRIMER_RIGHT_2_SEQUENCE=TCAATGATTCAGAGAGTGAGGG
PRIMER_LEFT_2=1117,22
PRIMER_RIGHT_2=1403,22
PRIMER_LEFT_2_TM=60.019
PRIMER_RIGHT_2_TM=59.284
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.7502
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.716126
PRIMER_LEFT_3_SEQUENCE=ATGAGGCTTTGATAGGTTGGAA
PRIMER_RIGHT_3_SEQUENCE=TCAATGATTCAGAGAGTGAGGG
PRIMER_LEFT_3=1158,22
PRIMER_RIGHT_3=1403,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.284
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=0.7701
PRIMER_LEFT_4_PENALTY=0.054021
PRIMER_RIGHT_4_PENALTY=0.716126
PRIMER_LEFT_4_SEQUENCE=AGTGACGGAGGTTAGTAACGGA
PRIMER_RIGHT_4_SEQUENCE=TCAATGATTCAGAGAGTGAGGG
PRIMER_LEFT_4=1275,22
PRIMER_RIGHT_4=1403,22
PRIMER_LEFT_4_TM=60.054
PRIMER_RIGHT_4_TM=59.284
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=129
=
PRIMER_SEQUENCE_ID=SSR_04136 Ps_contig_mira-and-tgicl-ass_franssen_32236
SEQUENCE=CCTTAAAAGCCTCACAACAAAAGGCTTGTTCCATAGTACAATCAAAAACATGAAGCTGATAGTNTAGTAAGAGAGTTTCAAACTGCCTTTGAAGGTACAAAAACCTCACACCATACAATCCAAGTCTTTTCATTATATATACAAGCTAACAGCATGACATAATTTTATTTATGCACATTGAAGGAAATAGAATAAAGCATTCTACATAGTTTACTCATTGTGCTTCGGCGACAGAGTAATGCATTTCAGTAACTGATTTTTTCAAGAAACTAGCTCCACAGTTTGAATCTTGGCTACAAACATCAACAGAGGCAAAATCAGAAGAACCTTCAATAACTTGAGAATCCGACAACTCATAGCACAGTTCAGAATGTTCACCAAGGTTGGGAGACTGTTTCTTATCTTTATCTTTGCCATTGCTCCCTCGAGTTGAATTTCCCTTGTTACCTTTCTTCAAACTTTTCTTTTCATTCTTCTTCTTCTTGGACTTCGGCTGTTTCTTGTTTTTGACAGTATGCGACACTAATGTAGGTATTGGATCATACACATCAGCAGCCCATTTCACTGTCAATTTCACACGCCCTTCCCCGTTTTTATCACGACTACCTTTCATGGCAGATTTAAGTTCCGAAGGGGCCGGCAGAGATATTGAACGAGAGTAAGCTTGATGGACAGAATGCTCAGAAGGTGATTCTGTAACAGATGCTGCCTCACGAGGATTCGGAAAGGTAGCCGATTTTTTCAAAGACACCATGTTGAGTTTTGCTTCTTTCGGTTTAATAACCTTGTTTTCCTCGACTACATTGCATATCTCATGCCCCTTAGATGCATGCTCCAATTTTTCAGAATCTTGTATATGTAACGACTCACTAAAAGTGAGTTCAAGACTACTTATGAAACTCCCATGCTTGTCATTAACAGAAGAACACACTTCCATTGAATCTATCAACCTATTAGGTACTATAACTAAACTACTCTTAGCAAAGTTTGAAGCTCTAGCACTCTCCAAACAGTAATAAAACAAGAAAATCACTAGATCCCAGCCAATTCTTCAAACGATCAACTAGTAGCTCGACGAAACAACAGCAGCAACAACAACTAGGATAATAATATAAAACCAAAAAAGAGTAAAGGGACGATACAAGAAACGAAAGTTCTTATGTTAACTAAGATTACCACGCCTATCACTTTTTTCAAGAGGTTTCAAACGATGAGATAAAGATCCATAATAAAAATTGGTGATGAAAGGAACCAAATGACGAAGAAGTTCAATAGGCACCGAAGGCATTATTATATGCTCTTCGAAGCCCTCCTAACAGTTTCACACCCAACAAAACGAAACAACGGTAAATCCAAAGGAAACAAACTCTGATGTGAATTAAGGGATTTGAGAACTAGAAACCTAACACAAACAAAAAAACCTATGACATCAAAATGGTTACAACATGAACTCAGCTTAGTCCATAGAATAATCAAGTTGATAAAATCAAGAAAAACGGTAGATCGGGAAAAACGTGATGGACACTGGATTAGGTTGTTGGAATCGGATAGGGTGGAAAAATGGGTTTTGTTCATTTTATAGGAAGGGGTTCGAGAAGGTCAAGCGTCAAGTTGTTTGAAATTTYTGTGTCT
TARGET=471,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04137 Ps_contig_mira-and-tgicl-ass_franssen_32240
SEQUENCE=AACATAATGGAAAATTNAATGCTAACCCTAAGTGGTTGGCCTCGTTCCTTTCAAACCCATACCATATTTATGTAGCATCAAATTCAACCAATTATCGAACCAAACTCAATTTGCACCATTTCTTTACACAAAAAATAACCAATTTGTTGTATCATAATAATGGAGAAGGGAATCGGTGTTGAATTAGCTACAAGGTCTTCTATTGAAATGAGAAATTCAGTTGATGATGATGAGGATTTAGACTGCAAGGTTTCATTTCTAAGGATTGTGGAGACTTTCCTGCGGTTGTTCACTATTGGTTTGTGTGTGACAGCTCTTGTTATTATGCTTAAGAATTCTGAGGAAAATGAGTATGGTTCTGTTGCTTATTCTGATCTTGGTGCTTTCAGGTATTTGGTGCATGCCAATGGCATTTGTGCAGGATATTCACTATTCTCAGTTGTCATTGTTGTTATGCCACGCCCTTCAACCATGCCTAGAGCTTGGGCTTTCTTTTTGCTTGACCAGGTGTTAACCTACATAATCCTGGCTGCTGGAGCTGTATTATCAGAGGTTCTATACCTTACTGAAAAGGGACTCCCTACAGCAGCGTGGAGTTCAGTTTGTGGATCATTTGGTTCATTTTGTCACAAGATCAAAGCATCATTAGCTATCACATTTGTTGCTGTGATTTGCTATATTTTGCTTTCACTTATTTCCTCTTATAGGCTATTTAGCAAATATGATTCACCATCATCACAAGTCAACAACTCCAACAAGGACATTGCTGCTTTCAATGGTTGATCCTCAAATATTTTGCTTGTAATGTAATGTGTACATTAATTATGTCCAAGTGATTTGGCTTAAAGTGCCTCTAAACTGAATAGCTTTAACAGTGTGCTTTTGAATGTCGGGTAACTCTGAGTGTAATGATCTAAACTTGTTTTTTTTCTGTGTTGACTGAAAGCAATATGTATGTTTATGTGTAGGACAATAGATAATAACTTAACAAGCTATAGGTGTTTGGATTGACNAATCATAGCTCCATTCATTGAGAAAGTAACTTTTTCTTT
TARGET=222,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2094
PRIMER_LEFT_PENALTY=0.128591
PRIMER_RIGHT_PENALTY=0.080808
PRIMER_LEFT_SEQUENCE=CTCGTTCCTTTCAAACCCATAC
PRIMER_RIGHT_SEQUENCE=GAGCTGTCACACACAAACCAAT
PRIMER_LEFT=40,22
PRIMER_RIGHT=315,22
PRIMER_LEFT_TM=59.871
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.2646
PRIMER_LEFT_1_PENALTY=0.183834
PRIMER_RIGHT_1_PENALTY=0.080808
PRIMER_LEFT_1_SEQUENCE=GAAGGGAATCGGTGTTGAATTA
PRIMER_RIGHT_1_SEQUENCE=GAGCTGTCACACACAAACCAAT
PRIMER_LEFT_1=164,22
PRIMER_RIGHT_1=315,22
PRIMER_LEFT_1_TM=60.184
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.4270
PRIMER_LEFT_2_PENALTY=0.042816
PRIMER_RIGHT_2_PENALTY=0.384229
PRIMER_LEFT_2_SEQUENCE=AATAATGGAGAAGGGAATCGGT
PRIMER_RIGHT_2_SEQUENCE=CAGGAAAGTCTCCACAATCCTT
PRIMER_LEFT_2=155,22
PRIMER_RIGHT_2=281,22
PRIMER_LEFT_2_TM=60.043
PRIMER_RIGHT_2_TM=59.616
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=127
PRIMER_PAIR_PENALTY_3=0.5128
PRIMER_LEFT_3_PENALTY=0.128591
PRIMER_RIGHT_3_PENALTY=0.384229
PRIMER_LEFT_3_SEQUENCE=CTCGTTCCTTTCAAACCCATAC
PRIMER_RIGHT_3_SEQUENCE=CAGGAAAGTCTCCACAATCCTT
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=281,22
PRIMER_LEFT_3_TM=59.871
PRIMER_RIGHT_3_TM=59.616
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.5681
PRIMER_LEFT_4_PENALTY=0.183834
PRIMER_RIGHT_4_PENALTY=0.384229
PRIMER_LEFT_4_SEQUENCE=GAAGGGAATCGGTGTTGAATTA
PRIMER_RIGHT_4_SEQUENCE=CAGGAAAGTCTCCACAATCCTT
PRIMER_LEFT_4=164,22
PRIMER_RIGHT_4=281,22
PRIMER_LEFT_4_TM=60.184
PRIMER_RIGHT_4_TM=59.616
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_04138 Ps_contig_mira-and-tgicl-ass_franssen_32243
SEQUENCE=AGTTNGGGGAGTCGGTTAACTGCCTTGAGTTTCTTGTTCTGTTATCACTGAACGACACGGAAAAGTGGAGTGAGCTAGCAACAATATCTCCACAGATTACACACTTTTCCCAACACACTCAAACTCAAAACCTCCAAAACCCCTTTCGATTCTTCTTCCACCTCCCAAATTACTATTATGGACTCGCTCACTCTCCCTTTCCCCACACTTCCCTCCGCCACTCACCGCCCAATTTTCAATCCACGCGCCGCTTTCGGTTTCCGCTGCTTCCACACCTCAGCTCATCTTCCAACGCTTCGTGGCTCGCCGCTCTTTTCCGCCAACACACTCACCGCTAATTCCGTTCCGCCAAAAAATGGTGTATACACTGTCGGTGACTTCATGACAAAGAAAGATGAATTACAAGTAGTCAAGCCCACAACAACTGTGGATGAAGCTTTGGACTCTCTTGTTGAACACAGAATAACTGGTTTTCCCGTGATTGATGATAACTGGAAGCTAGTTGGTGTTGTTTCTGATTATGACTTGTTAGCACTGGACTCTATTTCAGGTCATGGGAAAACAGATAACAGCAGCTTGTTTCCCGAAGTTGACAGTACCTGGAAAACTTTCAATGAGGTTCAGAGGCTACTGAGTAAGACCAACGGAAAGGTGATTGGTGAATTAATGACCACTGCCCCTATGGTTGTTCGTGAGACCACCAATCTTGAGGATGCTGCTAGATTGTTGCTGGAAACAAAGTTTCGACGCCTTCCAGTTGTAGATGCTGAGGGTAGATTGGTTGGGATTATCACAAGAGGAAATGTTGTAAGAGCTGCACTTCAAATGAAACGAGACAGCCAAAAGAAAGCATGATAGGATCTAGAAGATCAATCCAAAAATTTGTTGCTTGATTTAAAGCAGTGATTAAATTGAAGTGTTCACACAAGCTTTGCGCTTTGCTTGGATTAGAAGTAAAATTTTGGCTTAGGCATGTAAATGTTATATTATTATATTGTTTTGGTATCACACAGAATGGTTTGTCATGGACAATATTTAGAAAGAAAAATCCCAATGTTCACATTTGACAAGGACCACCTTGGTGATAATGATGTTGAAATGTGAATTTGTTGTATTAATCATGTTCCACGGACTAAGGATAAACTCCAGACACAAATATCAACCAACATTAATCTTGCTTCTATTGACAACTTTATACTAATTAAATAAATAAATCTTTAAACT
TARGET=1203,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04139 Ps_contig_mira-and-tgicl-ass_franssen_32245
SEQUENCE=CCGTAGAACAATACAAAGAATAAATTTACTAAGAAAGAATACAAAAGAAAAGAATTAAGTAGAAGCGATGGTGAAAAGCATATGCACAATCTCTTTCAGGCAGAGGAAGGAATTGTATTCACTTGTTGATATGATAGAATCTTCAAAGGGATCATCTAGGACCTGAGAGTTCATCTGGCTGGATATGTGAAGTTGAATCTACTACTATCCAATTTGCGTTAGGGTGATGCATGTGGTTGTTGTTAAAATCTTGTCGATACGTCTCTTCTTTCTGCCATTGCTCCCATTTTTCTGCCAAACCATCACCTTCTAGCATTCTCACCACCTCAGACATCTTTGGTCTTTCCATCGGCGACCCTTGTGTGCATAGTAAAGCCACTTGGATTAACTGCTCTACCTCATCATCGTCATAATTTCCCGTCAATTCTGCATCTACAAGTGTCTCCAACTTCTTGTCTTTGAGAAGTCCTTTAACCCAATCAAGCAACATGACATCATCATCATTGGCAAGACGTGCTAAATCGAAAGCCCTCTGTCCAGTTATTAGTTCAAGAAGCATCACACCGTATCCAAAAACATCAGTCTTTTCTGAAGACTTTCCAGTTGACAGGTACTCCGGTGCTATATGCCCAATTGTACCACGAACGGCAGTAGTAACATGTGTATCTTTATAATCCATAAGCTTTGCTAAACCAAAATCTCCAACAACTGCTTCGAAATCCTCATCCAACAATATATTTGCAGCTTTCACATCACGATGAATAATCTTAGGGTCACAATGATCGTGCAAGTAAGCAAGTCCCCTGGCAGATCCCAGCGCAATATTTTTCCGCATAGGCCACTCAAGTGGCGGATCAGATTCACTACGTTCT
TARGET=493,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0406
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=AGTCCTTTAACCCAATCAAGCA
PRIMER_RIGHT_SEQUENCE=AGCAGTTGTTGGAGATTTTGGT
PRIMER_LEFT=464,22
PRIMER_RIGHT=712,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.1382
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.137489
PRIMER_LEFT_1_SEQUENCE=AGTCCTTTAACCCAATCAAGCA
PRIMER_RIGHT_1_SEQUENCE=ATGAGGATTTCGAAGCAGTTGT
PRIMER_LEFT_1=464,22
PRIMER_RIGHT_1=725,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.137
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.1405
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.139753
PRIMER_LEFT_2_SEQUENCE=AGTCCTTTAACCCAATCAAGCA
PRIMER_RIGHT_2_SEQUENCE=ATTCATCGTGATGTGAAAGCTG
PRIMER_LEFT_2=464,22
PRIMER_RIGHT_2=762,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.140
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=0.1727
PRIMER_LEFT_3_PENALTY=0.132857
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=TAACCCAATCAAGCAACATGAC
PRIMER_RIGHT_3_SEQUENCE=AGCAGTTGTTGGAGATTTTGGT
PRIMER_LEFT_3=471,22
PRIMER_RIGHT_3=712,22
PRIMER_LEFT_3_TM=59.867
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.1764
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.175676
PRIMER_LEFT_4_SEQUENCE=AGTCCTTTAACCCAATCAAGCA
PRIMER_RIGHT_4_SEQUENCE=TACGGTGTGATGCTTCTTGAAC
PRIMER_LEFT_4=464,22
PRIMER_RIGHT_4=567,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=60.176
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=104
=
PRIMER_SEQUENCE_ID=SSR_04140 Ps_contig_mira-and-tgicl-ass_franssen_32248
SEQUENCE=TTAATATGGAGACCAAACTTANNRGGTTTTATAATTTTATAGAAACTAAATATACATCTACTCCTTAAAAAATATATAACGATTAAACAATACTAANTTATTATAAATGAATTATTATTTATTTATTTTACGGAAGTTCTCAATGCAAAGAAAGATCTTGATACTTGGTATCGTAGAGTAAAAACAATAGATACCAAAATAACACTTATTAATCAAATTTCAATAAATCACACCAGAATTCACCGGGAAACCGGCTCTTCCGGTTTGTGAGCCAACTCAACAAGCAAAGGTCGCAATTTATTTTTCCACAATTGCCTGCCAGGTTCCTCCCCCTTTCCACGTTTCTTCATTTCAACTACCACGAATTCATCCGTTAGCCGGTAAACCATAACAAGACCAAGCAAATTACCTTCTTGCCCTTCCAACTTTGCTCCAGCATTATTCTCCAGTCTTTTCACAACCACCGTCGCTCCCTTCACCACCTCCTCCACCCTCTCAATAATCCTCTCCGGTTTCTCCGCCGAAACAACCCATTCAGCTAAACCCGACCCGTTTTGATCCTCAAACATACCCGACATATCTAACCCGGTTGAAAACGAAATCAAATCAAACGCGTTCAAGGACTTAAACCCGGTTCGAGATTCTTCCAACTCCGGTTCCTTAACTAAAACCCGGTCTAGTTTATATCCACCCGACTTGAACCACGGGTCTTGAAGAATTTCATCAACGCTAATCCTTGTGGCAGGGTTCGTATCCAACAATCTTGATAAGAGATTCTTCAGATCACATGACGTCCATTTCGGGAAACGAAATTGACCACGGTAGATCTTCCGGTACAGCACGGTAACATTGTAATCATTGAACGGTAAATATCCTGCAGTAATAGCAAACAAAACGACGCCGCAGGACCATATATCAGCTTTCGCACCGTCGTAGCCTTTCTTCGCGAGAATCTCCGGTGCCACGTAGGATGGTGTACCACAAACGGTGTGAAGTAAACCGTCAGGACGGATCTGGTTTTTAACAGCGCTTAAACCGAAATCCGTTACCTTAAGGTTATCATTCTCGTCTAGCAAGAGATTATCGAGTTTGAGATCCCGGTGGAAAACACCGTGAGAGTGACAGTGTTTCACGGCGGAGATAAGCTGCCGGAAGTATCTCCGAGCGTGATCTTCTGTGAGTTTGTCTTTTTTAGCCACCTCTTCGAAGAGCTCGCCGGCAGAGGCCAATTCAACAATGAAGTAGATTTTGTTCTTTGACGCAAGCACTTCGAAGAGGTTGATGATGTTAGGGTGGTGGAGGCGACTGAGAATTGAGATCTCACGCTCAATGTTTGCCGAGAAACCTCCATTGACGAGTTTCTTCTTGTTCATGACTTTCACCGCCACGTTCTTCCCCGTTTCAACATTCGTCGCCTGGTACACCTTCGCTGAAGCACCAACTCCCAACAATCTTCCAATTTCGTATTTCTCAAACAAAACGACGCCGTTTTTCGACTTGTTAGTCGTTTGTGTTTGAATTTGTGATTCTTCTGCCATAACTGCGTCTTTTTGTACCTGATATCCAACTGGTTTGAGATGGATGTGGATTTGTTAGGATTTAGAGTTGCCGTGGTTATTGATTATTTCTGACGTGCTTTTGCAAATTAATGAATAATCTTTAAAAGCACTTCTAAGCTCTAAATAATAATAGCGGCTTGAACTTGAAGTGTATTTGAGAGAATGAAAAGGAAGAGTAATAAGCTATGTGGTGTGTCCCGA
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04141 Ps_contig_mira-and-tgicl-ass_franssen_32251
SEQUENCE=TCATATGAACTAAATAGCCNCAAACTGCTTGTACACACAGTGGAAAGGGGTTTACAAAAACCTTTTGGTTTGTAGCCTTCCTATAAGATGACTAATATATATAGATRTATATATACTAAAAATTGGTTTCCCCTACTCCCATGTACATATTCACCTTTATATAAATAATAATAATACAACTTCATAATTAGCCATACAATTATTGCCTAAAGGCTATAAAGAATTAATTAAACACATAAATTATTAAACAATATAATAATCAAATTAATCACTAAAGATTATAAAATCACGCAGCTTTAACTAGAAGGATCTATATAATAACTTCAGCCATATTATTTCACTGTTTCAAAGAATTCTTGTTATGAACTTGAGAATCCATTCTTGTGTCTTGAGATTGTTTTGGATATTTTGAGTATGAATTAGATCTTCCTTTCTTGTGCTTCCTTCTTGGTTGTGTTGCTTGTGGAATAAACACTCCTGTTCCTTTGCTTTCTCTTCTCACCAAAGCTTGCTCATAGAGGATATCAAACTGTGGAGGAGGATGAATTGCTCTAGAAAAAGCCTGAAAAGAGTTTTGAGGTTGAGGAACAAAAGGATCTTCATCTTCATCCATAATTAAGAGAGCAATTTCCTTGCTCAAATTTGCAAAAAATAAATCATCATCTAATTCAATAGCAACCTCCATAATAATAATAATAATAAAAAAGAAAGTCTAGTGGTTTTGCATGAAAATTTCTAATCTTTTACTTGTTCCCCGACTCTGTCGTTTGTATTACCTACTTGTCTTTT
TARGET=164,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04142 Ps_contig_mira-and-tgicl-ass_franssen_32251
SEQUENCE=TCATATGAACTAAATAGCCNCAAACTGCTTGTACACACAGTGGAAAGGGGTTTACAAAAACCTTTTGGTTTGTAGCCTTCCTATAAGATGACTAATATATATAGATRTATATATACTAAAAATTGGTTTCCCCTACTCCCATGTACATATTCACCTTTATATAAATAATAATAATACAACTTCATAATTAGCCATACAATTATTGCCTAAAGGCTATAAAGAATTAATTAAACACATAAATTATTAAACAATATAATAATCAAATTAATCACTAAAGATTATAAAATCACGCAGCTTTAACTAGAAGGATCTATATAATAACTTCAGCCATATTATTTCACTGTTTCAAAGAATTCTTGTTATGAACTTGAGAATCCATTCTTGTGTCTTGAGATTGTTTTGGATATTTTGAGTATGAATTAGATCTTCCTTTCTTGTGCTTCCTTCTTGGTTGTGTTGCTTGTGGAATAAACACTCCTGTTCCTTTGCTTTCTCTTCTCACCAAAGCTTGCTCATAGAGGATATCAAACTGTGGAGGAGGATGAATTGCTCTAGAAAAAGCCTGAAAAGAGTTTTGAGGTTGAGGAACAAAAGGATCTTCATCTTCATCCATAATTAAGAGAGCAATTTCCTTGCTCAAATTTGCAAAAAATAAATCATCATCTAATTCAATAGCAACCTCCATAATAATAATAATAATAAAAAAGAAAGTCTAGTGGTTTTGCATGAAAATTTCTAATCTTTTACTTGTTCCCCGACTCTGTCGTTTGTATTACCTACTTGTCTTTT
TARGET=684,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04143 Ps_contig_mira-and-tgicl-ass_franssen_32252
SEQUENCE=AACACCATAACATAATGTTTTTTAATGATTTCTCTCACACACTCCCACGTGTTCTCAAATTCTAGATAAATTAAATTTATTTTTTCTCCATTATTAATAGTATTTAGATTTTTTTTTTANATATATGGTGTGAAAGAACCAGTTTTTCAGTGAAAGATACGTTTTTTTCCGATACCCTTCTGATTAGAGGGAAAAATAGTGCTGTGATTCTAGAATAACTTGCATGCCCATTTGAATTTGAGTTTAAAGTTTGAACCTTTTTCATCATTCACTACCATTTACTGGTGGTAATAGTGTTGTTTTTGTGAGAGTGGAGGATTTGGTTATGGAGAGATACGAGGTTGTTAAGGATTTGGGAGTTGGGAATTTTGGAGTGGCGAGGCTTCTGAGGAACAAGGAGACTAAGGAGCTTGTTGCTATGAAGTACATCGAGCGTGGCCATAAGATTGATGAGAATGTGGCTAGAGAGATTATCAACCATAGGTCTCTTCGGCACCCGAATATAATTCGGTTTAAGGAGGTGGTTTTGACTCCTACTCATTTAGCAATAGTGATGGAGTATGCAGCTGGAGGTGAACTCTTTGAGAGGATATGTAATGCTGGACGGTTCAGCGAAGACGAGGCTAGATATTTCTTTCAGCAGCTGATATCTGGTGTTCATTACTGTCATGCTATGCAAATATGTCATAGAGATTTGAAGCTGGAAAATACCCTTTTAGATGGAAGCCCTGCGCCTCGCTTGAAAATTTGTGACTTTGGTTATTCCAAGTCATCTTTGCTTCATTCCCGACCCAAATCGACTGTTGGAACTCCGGCTTATATAGCACCGGAGGTTCTTTCTAGGAGGGAGTATGATGGCAAGTTGGCTGATGTGTGGTCGTGCGGTGTGACCCTGTATGTCATGCTGGTCGGAGCATATCCCTTTGAGGATCAAGATGATCCTAGAAATTTTAGGAAAACAATTCAGCGCATAATGGCTGTTCAATACAAAATCCCTGATTATGTTCACATATCTCAAGATTGCAAACACCTTCTATCTCGAATATTTGTTGCAAATCCATTAAAGAGAATCTCTCTCAGGGAAATCAAAAACCATCCATGGTTTTTAAAGAATCTTCCAAGGGAGCTAACTGAATCAGCTCAAGCGGCGTACTACCAGAGAGGCAATCCAAGTTTTTCTATTCAAAGTGTGGACGAGATAAATAAAATTGTGGGAGAGGCGAGAGAGCCTCCTCCGGTATCTAGGCCGGTCAAAGGCTTTGGCTGGGAAGGCGAGGAAGAAGAAGAAGAAGTGGAAGAAGAAGAGGTGGAGGAAGAGGAAGAGGAAGAAGATGAGTATGACAAAAGGGTGAAAGAGGTTCATGCAAGTGGAGAATTTCAAATCAGTTAAGAAATGTATTAGTATTGTTTTGTGGTAAAGGTCACTTATTTATGTGTACTATAATGTATAACTTGGTTTTAAACCTGTGTGAGAAAGTTTGACTTGTCACATTGTTCATGTATAATAATATTTTTTTCAAGTTAACC
TARGET=1277,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1425
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TACCAGAGAGGCAATCCAAGTT
PRIMER_RIGHT_SEQUENCE=TGAACCTCTTTCACCCTTTTGT
PRIMER_LEFT=1153,22
PRIMER_RIGHT=1361,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.1425
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=TACCAGAGAGGCAATCCAAGTT
PRIMER_RIGHT_1_SEQUENCE=AACCTCTTTCACCCTTTTGTCA
PRIMER_LEFT_1=1153,22
PRIMER_RIGHT_1=1359,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.1555
PRIMER_LEFT_2_PENALTY=0.151106
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=CGTACTACCAGAGAGGCAATCC
PRIMER_RIGHT_2_SEQUENCE=AATTCTCCACTTGCATGAACCT
PRIMER_LEFT_2=1148,22
PRIMER_RIGHT_2=1376,22
PRIMER_LEFT_2_TM=60.151
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=0.1633
PRIMER_LEFT_3_PENALTY=0.151106
PRIMER_RIGHT_3_PENALTY=0.012153
PRIMER_LEFT_3_SEQUENCE=CGTACTACCAGAGAGGCAATCC
PRIMER_RIGHT_3_SEQUENCE=TGAACCTCTTTCACCCTTTTGT
PRIMER_LEFT_3=1148,22
PRIMER_RIGHT_3=1361,22
PRIMER_LEFT_3_TM=60.151
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.1633
PRIMER_LEFT_4_PENALTY=0.151106
PRIMER_RIGHT_4_PENALTY=0.012153
PRIMER_LEFT_4_SEQUENCE=CGTACTACCAGAGAGGCAATCC
PRIMER_RIGHT_4_SEQUENCE=AACCTCTTTCACCCTTTTGTCA
PRIMER_LEFT_4=1148,22
PRIMER_RIGHT_4=1359,22
PRIMER_LEFT_4_TM=60.151
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_04144 Ps_contig_mira-and-tgicl-ass_franssen_32255
SEQUENCE=TAAAGGGTANAACGNAGARTANATYGGGGGAGATGGATGAATTGGAACGTGGACGGAAATTGAGTGAAGAGTAGCGTAGAACAAAAAAGATGCGGCCAAACGCCGCCGGTAACGGCAACGACGACCGATGGGGAACAGCCGGACCGGCGATTCGCAAGAAAGGAACGAGTGTTCGTCCATGGCTGGTTGTCGACGTTACCGGAGACGCACAGGTGGTTGAGGCCGGAAAACACGCCATCATGAGACGAACTGGCCTCCCGGCACGTGACCTTCGAATCCTCGATCCTCTCCTTTCCTATCCTTCCACCGTTCTCGGACGAGAGAGAGCAATCGTCATCAATTTGGAACACATCAAAGCTATTATCACCGCAAACGAAGTTCTTCTTCTCAATTCAAGAGATCCTTCCGTTACTCCTTTTGTTCAGGAGCTTCAAGCGAGGATCCTCCGTCATCACGAAGCAACCACTAATCCTCAAGAAGATTCTCACGGCAGCGGAATTAAGATTCTTCCGTTCGAATTCGTTGCGCTTGAGGCGTGTCTTGAAGCGGCTTGTAGCGTTTTGGAGAATGAAGCCAAGACTTTGGAGCAAGAGGCGCATCCTGCTCTTGATAAATTGACGTCGAAGATTAGTACTCTGAATTTGGAACGCGTGAGACAAATTAAGAGCCGTTTGGTTGCGATAACGGGGAGGGTTCAAAAGGTGAGAGATGAATTGGAACATTTGCTTGATGATGATGAGGATATGGCTGAAATGTATCTTACTGAAAAGATACTTCAACAGCAGTTGGAACAAACGTCTTCTGAGGAAGATATTGATGCTCATGATGATGATGATAATGATATTGATAGAGCAGAAGGTGTTGAAATATCTTTGGAAGGGCGGGTAGGAGGAGAGGCAGGTTATGAGGACGGGGATGAGGATGAGGATAATCAAAATCGAAATGCGGGAGAACATATGTTTGGTGCTACAAATCAAATTGGTAGGGATAGCCGCGGCGGAACAAGGGCGAGCACTACTTATAGTGCCACCACCAATAAGCTTGATGTTGAGGAGCTTGAGATGCTCTTGGAAGCATACTTTGTTCAAATTGATGGAACTCTCAACAAGCTTTCCACGTTGAGGGAGTACGTCGATGACACAGAGGATTACATCAATATTATGCTGGATGACAAACAGAACCATCTCCTCCAAATGGGAGTCATGTTGACCACGGCAACTCTGGTAGTGAGTGCCTTTGTTGTTGTGGCTGGTGTTTTTGGCATGAATATTCATATTGAACTGTTTGATGATAAGCTCTACGGGACGAGAGAGTTTATGTGGACTGTTGGTGGCGGCACTGCTGGGACCATATTCTTGTATGTGGTTGCCATTGCCTGGTGCAAGTATAAACGCTTGCTAGAATAAGGTGCACTCCATTGTGTTCCATACAATAACGTAATTTAATTGGAAAATCGACTTTTTTGTATGTTCTGTAAAATTTTAATACAAGACTTATACATCATCTATTTGCTTCTTCTTTTTCCTGGACATTATTGTATGAACGAAGTTTGCAACAACATTGGTTTTATGCAACACTTGCTGCCAGTCATGTAATATTCACAAAGATTTCTTGAGATTCTATAGTTANTTTT
TARGET=728,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04145 Ps_contig_mira-and-tgicl-ass_franssen_32255
SEQUENCE=TAAAGGGTANAACGNAGARTANATYGGGGGAGATGGATGAATTGGAACGTGGACGGAAATTGAGTGAAGAGTAGCGTAGAACAAAAAAGATGCGGCCAAACGCCGCCGGTAACGGCAACGACGACCGATGGGGAACAGCCGGACCGGCGATTCGCAAGAAAGGAACGAGTGTTCGTCCATGGCTGGTTGTCGACGTTACCGGAGACGCACAGGTGGTTGAGGCCGGAAAACACGCCATCATGAGACGAACTGGCCTCCCGGCACGTGACCTTCGAATCCTCGATCCTCTCCTTTCCTATCCTTCCACCGTTCTCGGACGAGAGAGAGCAATCGTCATCAATTTGGAACACATCAAAGCTATTATCACCGCAAACGAAGTTCTTCTTCTCAATTCAAGAGATCCTTCCGTTACTCCTTTTGTTCAGGAGCTTCAAGCGAGGATCCTCCGTCATCACGAAGCAACCACTAATCCTCAAGAAGATTCTCACGGCAGCGGAATTAAGATTCTTCCGTTCGAATTCGTTGCGCTTGAGGCGTGTCTTGAAGCGGCTTGTAGCGTTTTGGAGAATGAAGCCAAGACTTTGGAGCAAGAGGCGCATCCTGCTCTTGATAAATTGACGTCGAAGATTAGTACTCTGAATTTGGAACGCGTGAGACAAATTAAGAGCCGTTTGGTTGCGATAACGGGGAGGGTTCAAAAGGTGAGAGATGAATTGGAACATTTGCTTGATGATGATGAGGATATGGCTGAAATGTATCTTACTGAAAAGATACTTCAACAGCAGTTGGAACAAACGTCTTCTGAGGAAGATATTGATGCTCATGATGATGATGATAATGATATTGATAGAGCAGAAGGTGTTGAAATATCTTTGGAAGGGCGGGTAGGAGGAGAGGCAGGTTATGAGGACGGGGATGAGGATGAGGATAATCAAAATCGAAATGCGGGAGAACATATGTTTGGTGCTACAAATCAAATTGGTAGGGATAGCCGCGGCGGAACAAGGGCGAGCACTACTTATAGTGCCACCACCAATAAGCTTGATGTTGAGGAGCTTGAGATGCTCTTGGAAGCATACTTTGTTCAAATTGATGGAACTCTCAACAAGCTTTCCACGTTGAGGGAGTACGTCGATGACACAGAGGATTACATCAATATTATGCTGGATGACAAACAGAACCATCTCCTCCAAATGGGAGTCATGTTGACCACGGCAACTCTGGTAGTGAGTGCCTTTGTTGTTGTGGCTGGTGTTTTTGGCATGAATATTCATATTGAACTGTTTGATGATAAGCTCTACGGGACGAGAGAGTTTATGTGGACTGTTGGTGGCGGCACTGCTGGGACCATATTCTTGTATGTGGTTGCCATTGCCTGGTGCAAGTATAAACGCTTGCTAGAATAAGGTGCACTCCATTGTGTTCCATACAATAACGTAATTTAATTGGAAAATCGACTTTTTTGTATGTTCTGTAAAATTTTAATACAAGACTTATACATCATCTATTTGCTTCTTCTTTTTCCTGGACATTATTGTATGAACGAAGTTTGCAACAACATTGGTTTTATGCAACACTTGCTGCCAGTCATGTAATATTCACAAAGATTTCTTGAGATTCTATAGTTANTTTT
TARGET=823,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04146 Ps_contig_mira-and-tgicl-ass_franssen_32259
SEQUENCE=CTAAATAGCAAGTANTCAATTCAAATTATCATAATAACAAAAACCTTCTACAGTTGGTTACAACAACACCTTGGTAAGACAGTTTACAAACTAGAACACAATAGGAAACCAGTAGAAAACAATGAGGCCACAGTCGTATTCAAAACAACTACCTAATGGAATCAATCTCAGAATATCCACACTCTATAATATTCGCAACAATTTGTGACACAATAGAAATGAATACAAATCAATCATAAAAATTTACTGTAATAACAGAGTTAATCTCAAAATCACAACACCACAAGCATACGAAATTGATATATTTACCGAAGCTGTGTCACTGTTGTCGTTTGTTGTTGCTGCGAAGCTGGAAGGTTGGTTGCTTGGTTTCGCCCGTTGGGGAAGAGAGAATCTGCGTCGCGGTTGGTCGTGGTGAATTTAGAAGAGCGAACCGCGGCGGCGGCGATGTTGGTGAACAGAGGTGGATTCGCCTGAGTGTGAAGGAGCTTGGTTATTGACGCGACGGTGAGGTTTGGAATTTGTCGCCGGAATAGATGAGGAAGATGGCAGATGGTTCAAAATTGACGGTGGTTGAGCGATGATGCGGTGACGTAGTGGTAGTGGTGGCGATTCACGGAGGTTAGTCTAGGTTGTTTTGGCGGTTGAAAAGGGGGTTTTGTGTTGTGTTAAAGGGTGTGTCGGTGAGGTTGTTGCGAGACGGAGGAAGACGATGGGTGAGTGGAAGCTATGGCCTCATAGTGGTAGTGGTGGCGATTCACGGTGGAGGATTTTTGGAAGATGATGGTGAATGAGGTCGAAGGATTGTGGGTGAAGTGGAGAGGATTTTGCGGTGGTCGCCGGAAAATGAGGTTTGTGAGGGAGATTTCTTCTTTTTCCTTCTTTTATTATTTTATCGTGAACATCCACCTTTTTCCTTTTCACTTTCTATTTCATTTTTTCTATCTCTGGAGGAGAGAGCATCCGTTAATATGTAACTACCCCCG
TARGET=436,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1602
PRIMER_LEFT_PENALTY=0.073900
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=CAACACCACAAGCATACGAAAT
PRIMER_RIGHT_SEQUENCE=TAACCAAGCTCCTTCACACTCA
PRIMER_LEFT=275,22
PRIMER_RIGHT=495,22
PRIMER_LEFT_TM=59.926
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.1602
PRIMER_LEFT_1_PENALTY=0.073900
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=ACACCACAAGCATACGAAATTG
PRIMER_RIGHT_1_SEQUENCE=TAACCAAGCTCCTTCACACTCA
PRIMER_LEFT_1=277,22
PRIMER_RIGHT_1=495,22
PRIMER_LEFT_1_TM=59.926
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=0.2075
PRIMER_LEFT_2_PENALTY=0.121136
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=AGCTGTGTCACTGTTGTCGTTT
PRIMER_RIGHT_2_SEQUENCE=TAACCAAGCTCCTTCACACTCA
PRIMER_LEFT_2=312,22
PRIMER_RIGHT_2=495,22
PRIMER_LEFT_2_TM=59.879
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=0.2075
PRIMER_LEFT_3_PENALTY=0.121136
PRIMER_RIGHT_3_PENALTY=0.086339
PRIMER_LEFT_3_SEQUENCE=AAGCTGTGTCACTGTTGTCGTT
PRIMER_RIGHT_3_SEQUENCE=TAACCAAGCTCCTTCACACTCA
PRIMER_LEFT_3=311,22
PRIMER_RIGHT_3=495,22
PRIMER_LEFT_3_TM=59.879
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=0.2447
PRIMER_LEFT_4_PENALTY=0.158317
PRIMER_RIGHT_4_PENALTY=0.086339
PRIMER_LEFT_4_SEQUENCE=TTTACCGAAGCTGTGTCACTGT
PRIMER_RIGHT_4_SEQUENCE=TAACCAAGCTCCTTCACACTCA
PRIMER_LEFT_4=304,22
PRIMER_RIGHT_4=495,22
PRIMER_LEFT_4_TM=59.842
PRIMER_RIGHT_4_TM=59.914
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_04147 Ps_contig_mira-and-tgicl-ass_franssen_32260
SEQUENCE=CCTACTGTTTCTCCATCAAAACTTTCTCTCTCTCTTTTCACACACAACAAAAAAAGAAATCTATTTCCTTTCTATATAACCCTATCAAAGATACATCAACAAAAACAAAAGAATATATTTTTTTTGCTAGTGTTTGATATTCATATGGGGTCTTCTTCTGTTTCTTCTTATGCTTTCACATTTATTACCATTCTCTTGTTACAGAATGTTGGTCACATTCATTGATACCTAAGCTATAATCAAAAGATTCATAAATTTCATAGCATTAAATTAAATTTCTCATATATTTATTTCATGGAGAGGCTTCAAGGACCCATCAATTCTTCTTTCTTTGGTGAAGTGAATTGTTTAGACCAAACATTACTTGACACAGAAAGTTTAAGATTTGAGGAAGATGAACAGTTTTTGTTATCAAGTTTAGAAGATAACATGCCATTTCTTCAAATGCTTCAAAGTGTTGAATCTCCGCAGTTAATTTATAAAGAACCAAACTTTCAAACGCTTTTGAGGCTTCAACATATGAAGAAACCATGGGAAGAAGACATGGCCTTTATTCCTAGAATGGATTCGCACCAACAAGTTCAAACAACATTGGAATTTGAAAGCTGTGTTACACATGATGTTCTAGAGATGCAGTCACCAGTGAAATCAGAAAGCTATGAACTTCAACACAAAGTTTCAGCTTCATGCATTGAGAAATTGAGCTATGAGTGTAACCAAGAAGAAACAAAAACAAAAACAACATGTTCCAAATCTCAACTTGGGACAACAAGGGAAAAGAGGAAGAGGAAAAGAACAAGACCGGTTAAGAATAAGGAAGATGTTGAGAATCAAAGGATGACACACATTGCTGTTGAACGCAATAGGAGACGACAAATGAATGACCATCTTAGTGTTCTTAGGTCTCTTATGCCATCTTCTTATATTCAAAGGGGTGACCAAGCATCAATAATTGGTGGAGCAATAGATTTTGTGAAGGAGTTAGAACAATTGCTTCAATCTCTTGAAGTACAAAAAAGGTTAAAAAAGAATGAAGAATTTGGATCTTCATCCTCCTCCTCATCACCACCAGAAGCTAGTTATGGAATGAAGTTATCATGTTGTGAAGAAAATGAGGTGAAGGCAGAAAACAAATCAGAAGTAGCAGATATAAAAGTGACATTGATTCAAACACATGTGAACTTGAAAATAGAGTGCAAAAGAAGATGTGGTCAATTGATAAAAGTAATTGTTGCTTTGGAGAATCTTAGGCTAACTATTCTTCACCTTAACATCACATCCTTTGAGTCTTCTGTACTTTACTCCCTCAATCTCAAGATTGAAGAAGATTGTAAGCTAGGCACAGCAAATGACATAGCAGAAGCAGTTCATGAAATATTCAACTACATTAATGGTAACTAGGTTTAACCATAGGGGAGTGAATAGAAGAAGAATAAAGGACAAGAAATTATTGAAGGGGAGAAAGAGGTAACGGGGAACATAGGGCAACAATTTCTTGTCTACATATATCAATGTATGATGTATCATGTTAATTAGGTAGCTCACAAAATAGAGATAGGGTACATCATGTTATTGTTTTTCTGTTTATTATATTGGAATCTTAGTTGGTAGTATCTTATACTAAAGTATGTGTAATGCTTCTTANNAANNTGTTAGTATTTTGGTTGATGAACT
TARGET=25,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04148 Ps_contig_mira-and-tgicl-ass_franssen_32261
SEQUENCE=AAARACCGCATCGAGTGGGAGTTTGCTCTCAATGTGTTAGAAAGAAACAGATCCGGTGAGCCCGCAGTTTCTCAACTCCAGGACATAAGCGACAGGTTTGGTTGGGATTATCTTGACAAACTTGGTTGGAGCAAAGTTGATTTCGACCTTCTAGGCACTTCTAATGGAGGGAGAATTCCGAGAGTAGACAATCGAAGGGACGCCGACGCCGACGCCAGTCAACYCAGTCTCCCACCTGCTACTACYAGTRACAATAGACCCAATCTCCGACCTGCTACTACTAGTAGGAGGATGGAGAGAAGAGAGAAACTGAAAATGAGATCGAGGACGACACATGATGAAGAAGAAGAAGCGCAGCAGCAGCACAAGGACAAGGACGATACTGTGCCTGCTGCTGATATTAAACAAATTGCCAAACCCATTCCCTGGACGTCAGGCTCTCCTCCGAAAAGCCATCAACTATCGCGCACAATCCTAAACAACTTTCTATCATCCATCTACTCTAATTAACTAATTCCATTTTTCTTTTTTCTTTTAATTAAGATTCTTGTAACACGCATTTTGTTATCCGTTTACTCTTATGAATATGTGACTTAGATGTTTAAAATTACAAGCACCTACGTTTTTTTTTCTTACAACAACTAAGAATAGAAATACTTTTTAAATTTTTTAGTTTCGGAAACG
TARGET=340,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04149 Ps_contig_mira-and-tgicl-ass_franssen_32261
SEQUENCE=AAARACCGCATCGAGTGGGAGTTTGCTCTCAATGTGTTAGAAAGAAACAGATCCGGTGAGCCCGCAGTTTCTCAACTCCAGGACATAAGCGACAGGTTTGGTTGGGATTATCTTGACAAACTTGGTTGGAGCAAAGTTGATTTCGACCTTCTAGGCACTTCTAATGGAGGGAGAATTCCGAGAGTAGACAATCGAAGGGACGCCGACGCCGACGCCAGTCAACYCAGTCTCCCACCTGCTACTACYAGTRACAATAGACCCAATCTCCGACCTGCTACTACTAGTAGGAGGATGGAGAGAAGAGAGAAACTGAAAATGAGATCGAGGACGACACATGATGAAGAAGAAGAAGCGCAGCAGCAGCACAAGGACAAGGACGATACTGTGCCTGCTGCTGATATTAAACAAATTGCCAAACCCATTCCCTGGACGTCAGGCTCTCCTCCGAAAAGCCATCAACTATCGCGCACAATCCTAAACAACTTTCTATCATCCATCTACTCTAATTAACTAATTCCATTTTTCTTTTTTCTTTTAATTAAGATTCTTGTAACACGCATTTTGTTATCCGTTTACTCTTATGAATATGTGACTTAGATGTTTAAAATTACAAGCACCTACGTTTTTTTTTCTTACAACAACTAAGAATAGAAATACTTTTTAAATTTTTTAGTTTCGGAAACG
TARGET=354,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04150 Ps_contig_mira-and-tgicl-ass_franssen_32263
SEQUENCE=AGATGATCATTGAACTTTCACAAGGTGGCTGGTTCAGGTGTTGTTGACCTGAGAGAGAGAGAGATGAAGGAACGCTCTATCGGTATGTATCAGTACGGGTTCGTCGTTACCGTGATTGTGATGTTGGCTCTGAGTTGCGGCGCTTCATCGGAACAACTGAGCTCAAGAGAATGCGAGAATCTCGGTTTCACCGGACTCGCTTTGTGCTCCGACTGCAATACTCTATCCGAGTATGTCAAAGACAAAGAGTTGGTATCGGATTGTCTCAAGTGTTGCGCGGAAGACTCCAATGATGCCACTACCAAGATTACATACTCTGGAGCATTATTGGAAGTTTGCATGAGGAAACTTGTTTTCTATCCTGAAATTGTTGGCTTTATTGAAGATGAAAAAGACCAGTTTCCATCAGTTAAAGTTCAATATGTTTTTAATTCTCCACCAAAGTTAATTATGCTTGATGATGTGGGCCAGCATAAGGAAACAATCAGAATTGATAATTGGAAACGCGAGCATATACTGCAGTATTTGCGAGAGAAGGTAAAACCTTCGGTGGGAGCTGCTTGAGAAGCTTCTATTCGATCTCTCCATGCTTTGCACTCTTGTTCTTGTCATAAGCTCCAAAAAAGTGAAAGGAGTATATACTGTAACTTAAACAAACCAGATAGACTCGCCCTTGTCACTGCATACTAGGGACAGAATAATTCAAATTATTCTGAGTTTTTGTGATAATTGATTCTGATAGGGCAGTTTACTTTTTTCCAGTTATGGTAACTGTAGAGACAACGTATTGTAACCCATAAATTGTTTCTTTTATGGCAACTGTAGAATAATGTGCACCTACTGTTTCAATATCAACTACTGGTTTTGGCATTTCTATAAGATCTGTACTTTGACTATTACTCATCATTGAGTGGATATGAATAAATCTAGGGTCAAAA
TARGET=51,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0152
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.006945
PRIMER_LEFT_SEQUENCE=TCATTGAACTTTCACAAGGTGG
PRIMER_RIGHT_SEQUENCE=CACTTGAGACAATCCGATACCA
PRIMER_LEFT=6,22
PRIMER_RIGHT=271,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.0468
PRIMER_LEFT_1_PENALTY=0.039879
PRIMER_RIGHT_1_PENALTY=0.006945
PRIMER_LEFT_1_SEQUENCE=ATTGAACTTTCACAAGGTGGCT
PRIMER_RIGHT_1_SEQUENCE=CACTTGAGACAATCCGATACCA
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=271,22
PRIMER_LEFT_1_TM=60.040
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.1320
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.123706
PRIMER_LEFT_2_SEQUENCE=TCATTGAACTTTCACAAGGTGG
PRIMER_RIGHT_2_SEQUENCE=AGATTCTCGCATTCTCTTGAGC
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=181,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.124
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=176
PRIMER_PAIR_PENALTY_3=0.1623
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.154023
PRIMER_LEFT_3_SEQUENCE=TCATTGAACTTTCACAAGGTGG
PRIMER_RIGHT_3_SEQUENCE=CCAACATCACAATCACGGTAAC
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=126,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=121
PRIMER_PAIR_PENALTY_4=0.1636
PRIMER_LEFT_4_PENALTY=0.039879
PRIMER_RIGHT_4_PENALTY=0.123706
PRIMER_LEFT_4_SEQUENCE=ATTGAACTTTCACAAGGTGGCT
PRIMER_RIGHT_4_SEQUENCE=AGATTCTCGCATTCTCTTGAGC
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=181,22
PRIMER_LEFT_4_TM=60.040
PRIMER_RIGHT_4_TM=60.124
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=174
=
PRIMER_SEQUENCE_ID=SSR_04151 Ps_contig_mira-and-tgicl-ass_franssen_32264
SEQUENCE=TCGGGGGTTTTCGTTTCGTTATCTTCTCCGTCTTGTTTTACGTAACCCTAACTCCGAACACAACCATTCTCTGCCTCTCGCCTCTTTATTTCTTCTCCAAACCCTAATTTCAATTTACCCCATTTTTCACATTTTCATCTTATCACAGATCTCAACCAGCTCCGAAACCACCTTTGAATTTCAATATACAAACAACCGATTCACGATTTTCCCTAGAAATTTTCGATTTTAGTTGATTCCGATTCCGATTCCGTTACTATTTTGATTTGAACCGACAATACAAACCGAAGCTATTACTACTGTAATTAACGCTTAAACGCTTAAAGCAGTTTTTATTTTGTTGTTGAATTGTTGGTGATTGACAATGGTTGGTGGTGGAAGCAGAAGGGACGATGGTTCGTTGGTAATTAACAACACCAATGTTTTTGCGGCGCTTGATACCTTGAAGAAGAAGAAGAAATCGGATAAGGAGAGAAAGAGCAAGGGTTCCAAATCGGTGAAATTGGAATCTGAATCGGATTCCAAAGTGTTTTGGGCTGTGGCCCCACTGAATGCAACTTCATGGGCTGATGTGGATGATGATGATGATTATTATGCCACCACTGCACCGCCTCAATCTGTTTGGTCTGTTTCCGACCCCCAACACAGTGAGGACAAGCCAGAAATTTTTGAGGAGACTGAGAGTGAGGAAGATATTTTAGACGAAGGGGATGAGGAGGAGGAAGAGCAAGATCATGAACCAGAACCAGAGCACGATGTGAAACCTGAACCTGAGCTTAAGAAGCATGCTGAAGTTCCTGTGGTACCAAAGGAGGCAGAACGGCAACTCTCCAAAAAGGAAAGGAAGAAAAAGGAACTTGAAGAGCTTGAGGCTCTTTTAGCTGATTTTGGAGTTGCACCGAAAGAAAGTAATGATGGTCAAGGTCAAGAGGAGTCACAAGGCGCATCCCAGGATAAGAAAGGTGCTGATGGGGATGTA
TARGET=445,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4514
PRIMER_LEFT_PENALTY=0.431597
PRIMER_RIGHT_PENALTY=0.019805
PRIMER_LEFT_SEQUENCE=CGATTCACGATTTTCCCTAGAA
PRIMER_RIGHT_SEQUENCE=TTGCTCTTTCTCTCCTTATCCG
PRIMER_LEFT=196,22
PRIMER_RIGHT=482,22
PRIMER_LEFT_TM=60.432
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=9.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.5368
PRIMER_LEFT_1_PENALTY=0.431597
PRIMER_RIGHT_1_PENALTY=0.105201
PRIMER_LEFT_1_SEQUENCE=CGATTCACGATTTTCCCTAGAA
PRIMER_RIGHT_1_SEQUENCE=AACCCTTGCTCTTTCTCTCCTT
PRIMER_LEFT_1=196,22
PRIMER_RIGHT_1=487,22
PRIMER_LEFT_1_TM=60.432
PRIMER_RIGHT_1_TM=59.895
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.6421
PRIMER_LEFT_2_PENALTY=0.622338
PRIMER_RIGHT_2_PENALTY=0.019805
PRIMER_LEFT_2_SEQUENCE=CGATTCCGATTCCGTTACTATT
PRIMER_RIGHT_2_SEQUENCE=TTGCTCTTTCTCTCCTTATCCG
PRIMER_LEFT_2=239,22
PRIMER_RIGHT_2=482,22
PRIMER_LEFT_2_TM=59.378
PRIMER_RIGHT_2_TM=59.980
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=9.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.7275
PRIMER_LEFT_3_PENALTY=0.622338
PRIMER_RIGHT_3_PENALTY=0.105201
PRIMER_LEFT_3_SEQUENCE=CGATTCCGATTCCGTTACTATT
PRIMER_RIGHT_3_SEQUENCE=AACCCTTGCTCTTTCTCTCCTT
PRIMER_LEFT_3=239,22
PRIMER_RIGHT_3=487,22
PRIMER_LEFT_3_TM=59.378
PRIMER_RIGHT_3_TM=59.895
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.7702
PRIMER_LEFT_4_PENALTY=0.750366
PRIMER_RIGHT_4_PENALTY=0.019805
PRIMER_LEFT_4_SEQUENCE=GAACCGACAATACAAACCGAAG
PRIMER_RIGHT_4_SEQUENCE=TTGCTCTTTCTCTCCTTATCCG
PRIMER_LEFT_4=268,22
PRIMER_RIGHT_4=482,22
PRIMER_LEFT_4_TM=60.750
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_04152 Ps_contig_mira-and-tgicl-ass_franssen_32264
SEQUENCE=TCGGGGGTTTTCGTTTCGTTATCTTCTCCGTCTTGTTTTACGTAACCCTAACTCCGAACACAACCATTCTCTGCCTCTCGCCTCTTTATTTCTTCTCCAAACCCTAATTTCAATTTACCCCATTTTTCACATTTTCATCTTATCACAGATCTCAACCAGCTCCGAAACCACCTTTGAATTTCAATATACAAACAACCGATTCACGATTTTCCCTAGAAATTTTCGATTTTAGTTGATTCCGATTCCGATTCCGTTACTATTTTGATTTGAACCGACAATACAAACCGAAGCTATTACTACTGTAATTAACGCTTAAACGCTTAAAGCAGTTTTTATTTTGTTGTTGAATTGTTGGTGATTGACAATGGTTGGTGGTGGAAGCAGAAGGGACGATGGTTCGTTGGTAATTAACAACACCAATGTTTTTGCGGCGCTTGATACCTTGAAGAAGAAGAAGAAATCGGATAAGGAGAGAAAGAGCAAGGGTTCCAAATCGGTGAAATTGGAATCTGAATCGGATTCCAAAGTGTTTTGGGCTGTGGCCCCACTGAATGCAACTTCATGGGCTGATGTGGATGATGATGATGATTATTATGCCACCACTGCACCGCCTCAATCTGTTTGGTCTGTTTCCGACCCCCAACACAGTGAGGACAAGCCAGAAATTTTTGAGGAGACTGAGAGTGAGGAAGATATTTTAGACGAAGGGGATGAGGAGGAGGAAGAGCAAGATCATGAACCAGAACCAGAGCACGATGTGAAACCTGAACCTGAGCTTAAGAAGCATGCTGAAGTTCCTGTGGTACCAAAGGAGGCAGAACGGCAACTCTCCAAAAAGGAAAGGAAGAAAAAGGAACTTGAAGAGCTTGAGGCTCTTTTAGCTGATTTTGGAGTTGCACCGAAAGAAAGTAATGATGGTCAAGGTCAAGAGGAGTCACAAGGCGCATCCCAGGATAAGAAAGGTGCTGATGGGGATGTA
TARGET=575,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1287
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=CGGATAAGGAGAGAAAGAGCAA
PRIMER_RIGHT_SEQUENCE=CTCAAAAATTTCTGGCTTGTCC
PRIMER_LEFT=461,22
PRIMER_RIGHT=672,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.1287
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=CGGATAAGGAGAGAAAGAGCAA
PRIMER_RIGHT_1_SEQUENCE=CCTCAAAAATTTCTGGCTTGTC
PRIMER_LEFT_1=461,22
PRIMER_RIGHT_1=673,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.1943
PRIMER_LEFT_2_PENALTY=0.019805
PRIMER_RIGHT_2_PENALTY=0.174518
PRIMER_LEFT_2_SEQUENCE=CGGATAAGGAGAGAAAGAGCAA
PRIMER_RIGHT_2_SEQUENCE=ATTTCTGGCTTGTCCTCACTGT
PRIMER_LEFT_2=461,22
PRIMER_RIGHT_2=665,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=60.175
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.2141
PRIMER_LEFT_3_PENALTY=0.105201
PRIMER_RIGHT_3_PENALTY=0.108929
PRIMER_LEFT_3_SEQUENCE=AAGGAGAGAAAGAGCAAGGGTT
PRIMER_RIGHT_3_SEQUENCE=CTCAAAAATTTCTGGCTTGTCC
PRIMER_LEFT_3=466,22
PRIMER_RIGHT_3=672,22
PRIMER_LEFT_3_TM=59.895
PRIMER_RIGHT_3_TM=60.109
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=0.2141
PRIMER_LEFT_4_PENALTY=0.105201
PRIMER_RIGHT_4_PENALTY=0.108929
PRIMER_LEFT_4_SEQUENCE=AAGGAGAGAAAGAGCAAGGGTT
PRIMER_RIGHT_4_SEQUENCE=CAAAAATTTCTGGCTTGTCCTC
PRIMER_LEFT_4=466,22
PRIMER_RIGHT_4=670,22
PRIMER_LEFT_4_TM=59.895
PRIMER_RIGHT_4_TM=60.109
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_04153 Ps_contig_mira-and-tgicl-ass_franssen_32266
SEQUENCE=AAGCACATCCCAACCGACCAACTAAACACGTGTCATCCACACGTCTTTCCTTTCTGGATTTTTAACATCGTTCATTCGAYNNNGTCGNGGGACATAACAATAACAACAACAACAACTTTATTTGCACCTTCACTTTATCGATACACTCTGCGAAGGTTGAAGCACAAGATGGCTTCAGCTACATCTTCTCTATGTTCATCTACCCTCCAATCCCAGATCAATGGACTCTCCCTTCGCAAAACCTCTCTTCTCCATTCCCCTTCTCTCACTTTCTCCAGGAGAAAATTTTCAACCGTGGTTAAGGCTTCATCTCGCGTGGACAAGTTTTCAAAAAGCGATATCATTGTTTCTCCATCTATTCTTTCCGCCAACTTTGCAAAATTGGGAGAACAGGTAAAAGCAGTGGAGTTGGCTGGTTGTGATTGGATTCATATTGATGTTATGGACGGTCGCTTTGTTCCAAACATTACAATTGGACCTCTTGTCGTTGATGCATTACGCCCTGTGACAGATTTACCTTTGGATGTACACCTGATGATTGTAGAACCAGACCAGCGGGTGCCAGATTTCATCAAAGCAGGAGCTGACATAATTAGTGTTCACTGTGAACAATCTTCCACTATCCATTTGCATCGTGCTGTTAATCAAGTAAAAAGCCTGGGAGCTAAAGCTGGAGTTGTCCTAAACCCTGGTACCCCACTAAGTGCGATAGAATATGTCCTTGATGTGGTTGATTTGGTCTTAATTATGTCTGTAAACCCTGGCTTTGGTGGCCAAAGTTTTATTGAGAGTCAAGTAAAGAAAATTGCTGAGTTGAGAAGATTGTGCGTGGAGAAGGGTGTCAATCCATGGATTGAAGTCGATGGTGGGGTTACCCCAGCAAATGCTTACAAGGTGATTGAGGCTGGAGCTAATGCACTAGTTGCTGGTTCTGCTGTATTTGGAGCTAAAGATTATGCTGAAGCTATAAAAGGGATCAAAGCGAGCAAAAGACCTGAACCAGTTGCTGTGTGAAATCCCACTGGTATCCTATCCATTTTAATCCAATACAATGTTCACGGTAAGCCGGCTTATTAGGCATGCCTTGCTATCACCACCAGCAATTGTTTCTCTATGGAATTAACTAGAAATCAGCCGAGTAATGCTTCTGCTGGGTTGCATGTTTGTGTATAATCTCTTGGAATATCAGGACATCTTTAATTCAAGATAAAGCAATCTACTGTCTGTAAGCTTGTAATTTATGGCTGTGGTCCACACCATATTGTAAAGAAGTTTGTGCTTTTTGAAATTACATATTAGAACTTGGATACCAATTCCAAATAC
TARGET=102,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04154 Ps_contig_mira-and-tgicl-ass_franssen_32272
SEQUENCE=CGATAATAAAACTCTTTTCTTTAATTCTCNRGRTCTTTTTCTGAATCTTTNAAATTCATTTAGAGTAATGGGAACTTAATAGGAGAAATTAATGGCATCCCTATGTATGAACTCATCGTTACCACTCTCTCCATCTTCTCATCCTCGCAACCTCACCCAAACCTCTCTTTCTTCTTCATCATCTTCTTCTCTGTTTGTTGATAACAACAACAACAATCATCATCATAATAGCCTTAGAACAACAAGTACCTTGAAGCCTATTGTTGTTAGTGGAAACCCTCCCACTTTTGTTTCCGCTCCCGGTCGCACTATTCTCGCTGTTGGAGATCTTCATGGAGATCTTAAGCAAGCTAGATCTGCACTTGAAATGGCTGGTGTGTTGAGCTCTGACGGTCAAGACTTGTGGACCGGTGGAGAAAATGTGTTGGTTCAACTTGGAGATATATTAGATCGAGGTGAAGATGAAATCGCTATCCTGTCCTTGCTACGGTCATTGGAGAAACAGGCAAAAGAAAACGGTGGAGCTGTGTTTCAGGTAAATGGAAACCATGAGACGATGAATGTGGAAGGGGATTTCAGATACGTTGAATCTGGTGGATTTGATGAATGTAGTGATTTCATGGAATATATCAATAATTCTGAAGATGACTGGGAAGAAACATTTACTGGTTGGGTCGATGTATCTGAAAAGTGGAAAGGAGATCGAACAATGTCAACAAGTAATTGGGGGCCTTGGAATTTAGTGAAGAGGCAAAAGGGAGTCATTGCTCGATCAATCCTCTTTAGGCCGGGTGGGCCACTGGCATGCGAGCTTGCAAGACACGCCGTTGTGCTTAAAGTTAATGATTGGGTCTTCTGTCATGGCGGCCTTCTTCCTCACCACGTTGCATATGGCTTAGAGCGGATGAACAAAGAAGTGTCTGAGTGGATGCGAGATCCAAGTAAGAATGACAGTACTTTCCAACTCCCTTTCATTGCCACCAGGGGTTATGACAGTGTTGTTTGGAATCGGTTGTATTCAAGAGACTCACCGGATTTGATGGACTATGAGGCTAATCAGGTATGTTCCATTCTCGAGGAAACACTGCAAGCAGTTGGTGCTAAGGCTATGGTGGTGGGACATACTCCTCAAACTACTGGTGTAAATTGTAAATACAATTGTAGCATATGGCGCGTAGATGTTGGAATGTCCAGCGGAGTCCTTAATTCAAGACCAGAGGTTCTAGAAATTATAGACGACAAAGCAAGAGTTATCAGGAGCGAAAGGGATACATACAGCGAACTTCAAGCGGCTGCATATACTTAATGACAGACAATTCCGAAAGCAGGGAAATAAAGCTATTTCTGAGTATTATGCTTCTGCAGAATTGAAGGCAAATATCTTTTTTGAAGTTAAATATAGCCTGTTAATGACTTGAAAGCAAGGTGAAAATAGGATGCAGAAGAATAAAAGTTGGAAAATAGGAAATATGAGGTGGTAAATAATCCAAATATTGTACTTAATTGATTATATTTTCACAATTACATTGAGTAGTCCCAAGTTAAATTGCTCCATTATGTTTGTTGTCATTTCAAATCTCTTGTGAAGTCAACAAGGGAAATCTATTGGAAGAGATTTGGTTCAGANAATTAAGAAGTAATTTAAAGAAACAA
TARGET=203,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04155 Ps_contig_mira-and-tgicl-ass_franssen_32279
SEQUENCE=AATAATAAACCATAACATTACACACATAACTAAAAAACCCAATCAAATCAAATCAAACCCTAATCCCTCACTTCTTAGCCTTCTTCACCGGAGTTTTAACGCTCTTCACCTTCTTCGCCGGACTTTTAACGCTCTTCGCCTTCTTCACAACCTTCTTAGCAACAGCCTTCTTCACCGGAGTAGCCTTCACGCTCTTAGCCTTTGGCTTCGCAACAGCTTTCGGCTTTGCCGCAGCTTTGGCTTTCGGCTTCCCAGCAGCTTTAGCCTTGGGCTTTGTGACAGCTTTAGCGCTTGGCTTGGTAACAGCTTTAGGAGCTTTGGTAGCAGCCGATGTCTTGGCCGGAGCAGTTTTTGCGGCCGCCGGAGCGAGTTTGAAGGAGCTTTTCACCTTGACAAGCTTTCCTGAAGCGACGGATTTCTTGAGATGRAGGAGAACAAGCTTACGGTAAGTTGGAGGTAAGTCCTTGTGTTTCTCTTCGATGAATTTTGTTATAGCATATTGGCTTGAACCGGTTCTTTCTTTCAGGCTTGTGATTGCTTCAGTTATCATCTTCAAAAAGAAATAAGAAAAAACAAAATCAATATACACACCCAAAAAATATATTCAAAATTACTGAAAATTGAACGGGATTTTGATTTCTACCTCAGCGAAAGTGGGATGAGAAAGTGGCTTCTTTGCTGCAGCGGTTTTCTTTGGCTTGGTTTGAGCGACGGTCGACATGGCTGAAAATGGAGGAGAGGGAATAACCGTTGA
TARGET=41,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04156 Ps_contig_mira-and-tgicl-ass_franssen_32280
SEQUENCE=TGAGGAAAACAGAAAACCCATTTCATTAAGACGGTGTCGAGTACAAAGAACACAAGATGTGTTTCTCCTAAACAATTTACAGCAACAACCTCCTAACACTAATTTTCATCTCTAATCTTTCTAAAACCAAACCATAATAATAATAAACCATAACATTACACACATAACTAAAAAACCCAATCAAATCAAATCAAACCCTAATCCCTCACTTCTTAGCCTTCTTCACCGGAGTTTTAACGCTCTTCACCTTCTTCGCCGGACTTTTAACGCTCTTCGCCTTCTTCACAACCTTCTTAGCAACAGCCTTCTTCACCGGAGTAGCCTTCACGCTCTTAGCCTTTGGCTTCGCAACAGCTTTCGGCTTCCCAGCAGCTTTAGCCTTGGGCTTTGTGACAGCTTTAGCGCTTGGCTTGGTAACAGCTTTAGGAGCTTTGGTAGCAGCCGATGTCTTGGCCGGAGCAGTTTTTGCGGCTGCCGGAGCGAGTTTGAAGGAGCTTTTCACCTTGACAAGCTTTCCTGAAGCGACGGATTTCTTGAGATGGAGGAGAACAAGCTTACGGTAAGTTGGAGGTAAGTCCTTGTATTTCTCTTCGATGAATTTTGTTATAGCATATTGGCTTGAACCGGTTCTTTCTTTCAGGCTTGTGATTGCTTCAGTTATCATCTCAGCGAAAGTGGGATGAGAAAGTGGCTTCTTTGCTGCAGCGGTTTTCTTGGGCTTGGTTTGAGCGACGGTCGACATGGCTGAAAATGGAGGAGAGGGAATAACCGTTGATGAGAGGGAATGAGAGATATTGAGAAGATTGAAGAGAATGTGATGATGTCCCCGAC
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1610
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.052069
PRIMER_LEFT_SEQUENCE=AACCCATTTCATTAAGACGGTG
PRIMER_RIGHT_SEQUENCE=AAGGCTGTTGCTAAGAAGGTTG
PRIMER_LEFT=14,22
PRIMER_RIGHT=306,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.2642
PRIMER_LEFT_1_PENALTY=0.108929
PRIMER_RIGHT_1_PENALTY=0.155310
PRIMER_LEFT_1_SEQUENCE=AACCCATTTCATTAAGACGGTG
PRIMER_RIGHT_1_SEQUENCE=CGTTAAAACTCCGGTGAAGAAG
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=238,22
PRIMER_LEFT_1_TM=60.109
PRIMER_RIGHT_1_TM=60.155
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=0.2857
PRIMER_LEFT_2_PENALTY=0.108929
PRIMER_RIGHT_2_PENALTY=0.176730
PRIMER_LEFT_2_SEQUENCE=AACCCATTTCATTAAGACGGTG
PRIMER_RIGHT_2_SEQUENCE=TGAGGGATTAGGGTTTGATTTG
PRIMER_LEFT_2=14,22
PRIMER_RIGHT_2=207,22
PRIMER_LEFT_2_TM=60.109
PRIMER_RIGHT_2_TM=60.177
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.3499
PRIMER_LEFT_3_PENALTY=0.297793
PRIMER_RIGHT_3_PENALTY=0.052069
PRIMER_LEFT_3_SEQUENCE=ACGGTGTCGAGTACAAAGAACA
PRIMER_RIGHT_3_SEQUENCE=AAGGCTGTTGCTAAGAAGGTTG
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=306,22
PRIMER_LEFT_3_TM=59.702
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.3499
PRIMER_LEFT_4_PENALTY=0.297793
PRIMER_RIGHT_4_PENALTY=0.052069
PRIMER_LEFT_4_SEQUENCE=CGGTGTCGAGTACAAAGAACAC
PRIMER_RIGHT_4_SEQUENCE=AAGGCTGTTGCTAAGAAGGTTG
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=306,22
PRIMER_LEFT_4_TM=59.702
PRIMER_RIGHT_4_TM=59.948
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_04157 Ps_contig_mira-and-tgicl-ass_franssen_32280
SEQUENCE=TGAGGAAAACAGAAAACCCATTTCATTAAGACGGTGTCGAGTACAAAGAACACAAGATGTGTTTCTCCTAAACAATTTACAGCAACAACCTCCTAACACTAATTTTCATCTCTAATCTTTCTAAAACCAAACCATAATAATAATAAACCATAACATTACACACATAACTAAAAAACCCAATCAAATCAAATCAAACCCTAATCCCTCACTTCTTAGCCTTCTTCACCGGAGTTTTAACGCTCTTCACCTTCTTCGCCGGACTTTTAACGCTCTTCGCCTTCTTCACAACCTTCTTAGCAACAGCCTTCTTCACCGGAGTAGCCTTCACGCTCTTAGCCTTTGGCTTCGCAACAGCTTTCGGCTTCCCAGCAGCTTTAGCCTTGGGCTTTGTGACAGCTTTAGCGCTTGGCTTGGTAACAGCTTTAGGAGCTTTGGTAGCAGCCGATGTCTTGGCCGGAGCAGTTTTTGCGGCTGCCGGAGCGAGTTTGAAGGAGCTTTTCACCTTGACAAGCTTTCCTGAAGCGACGGATTTCTTGAGATGGAGGAGAACAAGCTTACGGTAAGTTGGAGGTAAGTCCTTGTATTTCTCTTCGATGAATTTTGTTATAGCATATTGGCTTGAACCGGTTCTTTCTTTCAGGCTTGTGATTGCTTCAGTTATCATCTCAGCGAAAGTGGGATGAGAAAGTGGCTTCTTTGCTGCAGCGGTTTTCTTGGGCTTGGTTTGAGCGACGGTCGACATGGCTGAAAATGGAGGAGAGGGAATAACCGTTGATGAGAGGGAATGAGAGATATTGAGAAGATTGAAGAGAATGTGATGATGTCCCCGAC
TARGET=179,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1610
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.052069
PRIMER_LEFT_SEQUENCE=AACCCATTTCATTAAGACGGTG
PRIMER_RIGHT_SEQUENCE=AAGGCTGTTGCTAAGAAGGTTG
PRIMER_LEFT=14,22
PRIMER_RIGHT=306,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.2642
PRIMER_LEFT_1_PENALTY=0.108929
PRIMER_RIGHT_1_PENALTY=0.155310
PRIMER_LEFT_1_SEQUENCE=AACCCATTTCATTAAGACGGTG
PRIMER_RIGHT_1_SEQUENCE=CGTTAAAACTCCGGTGAAGAAG
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=238,22
PRIMER_LEFT_1_TM=60.109
PRIMER_RIGHT_1_TM=60.155
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=0.3499
PRIMER_LEFT_2_PENALTY=0.297793
PRIMER_RIGHT_2_PENALTY=0.052069
PRIMER_LEFT_2_SEQUENCE=ACGGTGTCGAGTACAAAGAACA
PRIMER_RIGHT_2_SEQUENCE=AAGGCTGTTGCTAAGAAGGTTG
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=306,22
PRIMER_LEFT_2_TM=59.702
PRIMER_RIGHT_2_TM=59.948
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.3499
PRIMER_LEFT_3_PENALTY=0.297793
PRIMER_RIGHT_3_PENALTY=0.052069
PRIMER_LEFT_3_SEQUENCE=CGGTGTCGAGTACAAAGAACAC
PRIMER_RIGHT_3_SEQUENCE=AAGGCTGTTGCTAAGAAGGTTG
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=306,22
PRIMER_LEFT_3_TM=59.702
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.4531
PRIMER_LEFT_4_PENALTY=0.297793
PRIMER_RIGHT_4_PENALTY=0.155310
PRIMER_LEFT_4_SEQUENCE=ACGGTGTCGAGTACAAAGAACA
PRIMER_RIGHT_4_SEQUENCE=CGTTAAAACTCCGGTGAAGAAG
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=238,22
PRIMER_LEFT_4_TM=59.702
PRIMER_RIGHT_4_TM=60.155
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_04158 Ps_contig_mira-and-tgicl-ass_franssen_32282
SEQUENCE=GGTTCACCTCCGTCGTGGTGGTCAAGCCGTGGATTCAGTACAAAAAAACATGGATTGAAGCAGTTTGAAGGTGAAGGAATTGGTTGAGAGAAGGTTGAAGGAATTGGTTGAGAGAAGGTTGAAGGGCTCAATTGAAGGTGAGAGTTGATATTGAAGGGATGATTATCTGAGAATGGATAGAAGGTGGTGTTGTTGCAGAGCGAAAATTCCCTACTGCAGAATTGAAAAGAACTCTCTGACAAAAATACCAAAAAAAAGCTCCTTCTCTCAATGATCAGCCAGGTATTTATAGATTCATTCTTCCTTTACTATGCTTCCAATCAATTAGAGTCGGTTGAGAATCTGTTACGGTTTTGGTTGGTTACAAAATAGTTAGGATTTCTGTTAGGTTAGTTAGGAATCGGTTAGAGGTCTGGAGTTAGTTACGGTTGGTTAAAAATGGTTTTCTCCTTATGTTATTTTCGCTGGTTCAGTTATGCAGTTAGAAACTGCTATGCTTGTGATGTGTCATACGGGATAAATGCTTCAAATTCGATCTTTTACTGAACTTTATGGTTGTCTCATGTTTCGCATTTGTCTTTGAAATTGTACTTGGTAGGGAGTGTGGTGCGGTAATAGGTGGGATAGGATAGGATAGGATAACCATCATTCCAAAACTTCATATATCATTATCGTGTCATTGTGATTTCTGCTTATTTGAATAAAATTTGTTCAATTTCTGTTAGTAGCTGTTGGCAATGTTATTCTGAAATTCAGCTTAGATTGCAATGCCAATTACTTTTGTTTTCTGCTGTTACGTGAATATGGTTAAAGTATTAACTTTGGTTAAAAATATGTTAGAATGATTTATGTTAGAATTAATGCTCTATTCTGCCAAAATGTTAGCCCTTGTTTACAGTTCCAATTCGGTTGGCTAGTTAGTTTGGACGGAAAGTTCTTACTTTGTGAGCACTTGGTTAATTTGTTGTGGTATTGGCTTGGAATCTTGATGTTGGATATGACACGACCTGCTTTAGATAGTTAAGGACTATTGGCTCTGTTTTGAAAATGGATTTTATTTGCTAGCCTTGGAGTTAATCGGTGAAAGTGTTTTGCATGATGCTTGGATGATTTGTGAACTTATGGTGCACTTGGGATGCTTTGAATATTGGAGTGAAACTTGGTTGCTTGCTCTATGCACTTGTATTGAAGAGTGGAGCAGTGGGTGGATGCTTAATTAAACTTTCATTATGTTATTGATRCTAAATTTTGTTTGTAATTTTAGTAAATTAAA
TARGET=622,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04159 Ps_contig_mira-and-tgicl-ass_franssen_32283
SEQUENCE=AAGCAGTGGGTAGTCAACGCAGAGTCGGGGATTCTCACTTTCACGCCAATTAACTTTTGGGCGGTGAGCGAGCGAGCGAGCGAACGAACAAACGGTCGTTACGTTCTCCACCGTCGCATTCTTCTTCTTCTTCCTTGCGCCTCTTTGCTTTTCCTGGAAAAATTAACACACGAAGCTATCGTTTTTGTTAACGGAACCAAACGGGACGTGACTCTAGGCTTCAAGTGCGCAGAAGAAGGTTGTTGCAGAAGCCATGGTTCTTGACACCATTCTGTCTTCTTCTTCTCCTCGTTTAAAGTCTCCATCTTTCCGTAGACCTAAGGATGGACTTGGTAGTTGGTCAACACTTTTCCAGAGGCACCGCTACCTCTTATCTGCTCTCGTGCTCCTTACTATTCTATGTACTGTTTATCTTTACTTCGCCATCACTATAGGAAGTGGCTCCTGTTCTGGGTTGAG
TARGET=66,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7900
PRIMER_LEFT_PENALTY=0.729140
PRIMER_RIGHT_PENALTY=0.060824
PRIMER_LEFT_SEQUENCE=TCTCACTTTCACGCCAATTAAC
PRIMER_RIGHT_SEQUENCE=GCTTCTGCAACAACCTTCTTCT
PRIMER_LEFT=32,22
PRIMER_RIGHT=251,22
PRIMER_LEFT_TM=59.271
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.8025
PRIMER_LEFT_1_PENALTY=0.741655
PRIMER_RIGHT_1_PENALTY=0.060824
PRIMER_LEFT_1_SEQUENCE=AGTGGGTAGTCAACGCAGAGTC
PRIMER_RIGHT_1_SEQUENCE=GCTTCTGCAACAACCTTCTTCT
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=251,22
PRIMER_LEFT_1_TM=60.742
PRIMER_RIGHT_1_TM=60.061
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=1.0358
PRIMER_LEFT_2_PENALTY=0.975014
PRIMER_RIGHT_2_PENALTY=0.060824
PRIMER_LEFT_2_SEQUENCE=AGAGTCGGGGATTCTCACTTTC
PRIMER_RIGHT_2_SEQUENCE=GCTTCTGCAACAACCTTCTTCT
PRIMER_LEFT_2=20,22
PRIMER_RIGHT_2=251,22
PRIMER_LEFT_2_TM=60.975
PRIMER_RIGHT_2_TM=60.061
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=1.1142
PRIMER_LEFT_3_PENALTY=0.729140
PRIMER_RIGHT_3_PENALTY=0.385098
PRIMER_LEFT_3_SEQUENCE=TCTCACTTTCACGCCAATTAAC
PRIMER_RIGHT_3_SEQUENCE=AGGAAGAAGAAGAAGAATGCGA
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=134,22
PRIMER_LEFT_3_TM=59.271
PRIMER_RIGHT_3_TM=59.615
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=1.1268
PRIMER_LEFT_4_PENALTY=0.741655
PRIMER_RIGHT_4_PENALTY=0.385098
PRIMER_LEFT_4_SEQUENCE=AGTGGGTAGTCAACGCAGAGTC
PRIMER_RIGHT_4_SEQUENCE=AGGAAGAAGAAGAAGAATGCGA
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=134,22
PRIMER_LEFT_4_TM=60.742
PRIMER_RIGHT_4_TM=59.615
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=131
=
PRIMER_SEQUENCE_ID=SSR_04160 Ps_contig_mira-and-tgicl-ass_franssen_32283
SEQUENCE=AAGCAGTGGGTAGTCAACGCAGAGTCGGGGATTCTCACTTTCACGCCAATTAACTTTTGGGCGGTGAGCGAGCGAGCGAGCGAACGAACAAACGGTCGTTACGTTCTCCACCGTCGCATTCTTCTTCTTCTTCCTTGCGCCTCTTTGCTTTTCCTGGAAAAATTAACACACGAAGCTATCGTTTTTGTTAACGGAACCAAACGGGACGTGACTCTAGGCTTCAAGTGCGCAGAAGAAGGTTGTTGCAGAAGCCATGGTTCTTGACACCATTCTGTCTTCTTCTTCTCCTCGTTTAAAGTCTCCATCTTTCCGTAGACCTAAGGATGGACTTGGTAGTTGGTCAACACTTTTCCAGAGGCACCGCTACCTCTTATCTGCTCTCGTGCTCCTTACTATTCTATGTACTGTTTATCTTTACTTCGCCATCACTATAGGAAGTGGCTCCTGTTCTGGGTTGAG
TARGET=119,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5049
PRIMER_LEFT_PENALTY=0.444125
PRIMER_RIGHT_PENALTY=0.060824
PRIMER_LEFT_SEQUENCE=AACAAACGGTCGTTACGTTCTC
PRIMER_RIGHT_SEQUENCE=GCTTCTGCAACAACCTTCTTCT
PRIMER_LEFT=86,22
PRIMER_RIGHT=251,22
PRIMER_LEFT_TM=60.444
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.7860
PRIMER_LEFT_1_PENALTY=0.444125
PRIMER_RIGHT_1_PENALTY=0.341875
PRIMER_LEFT_1_SEQUENCE=AACAAACGGTCGTTACGTTCTC
PRIMER_RIGHT_1_SEQUENCE=GAGAGCAGATAAGAGGTAGCGG
PRIMER_LEFT_1=86,22
PRIMER_RIGHT_1=381,22
PRIMER_LEFT_1_TM=60.444
PRIMER_RIGHT_1_TM=59.658
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=0.7900
PRIMER_LEFT_2_PENALTY=0.729140
PRIMER_RIGHT_2_PENALTY=0.060824
PRIMER_LEFT_2_SEQUENCE=TCTCACTTTCACGCCAATTAAC
PRIMER_RIGHT_2_SEQUENCE=GCTTCTGCAACAACCTTCTTCT
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=251,22
PRIMER_LEFT_2_TM=59.271
PRIMER_RIGHT_2_TM=60.061
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.8025
PRIMER_LEFT_3_PENALTY=0.741655
PRIMER_RIGHT_3_PENALTY=0.060824
PRIMER_LEFT_3_SEQUENCE=AGTGGGTAGTCAACGCAGAGTC
PRIMER_RIGHT_3_SEQUENCE=GCTTCTGCAACAACCTTCTTCT
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=251,22
PRIMER_LEFT_3_TM=60.742
PRIMER_RIGHT_3_TM=60.061
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=248
PRIMER_PAIR_PENALTY_4=0.8567
PRIMER_LEFT_4_PENALTY=0.444125
PRIMER_RIGHT_4_PENALTY=0.412596
PRIMER_LEFT_4_SEQUENCE=AACAAACGGTCGTTACGTTCTC
PRIMER_RIGHT_4_SEQUENCE=GAGCAGATAAGAGGTAGCGGTG
PRIMER_LEFT_4=86,22
PRIMER_RIGHT_4=379,22
PRIMER_LEFT_4_TM=60.444
PRIMER_RIGHT_4_TM=60.413
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_04161 Ps_contig_mira-and-tgicl-ass_franssen_32283
SEQUENCE=AAGCAGTGGGTAGTCAACGCAGAGTCGGGGATTCTCACTTTCACGCCAATTAACTTTTGGGCGGTGAGCGAGCGAGCGAGCGAACGAACAAACGGTCGTTACGTTCTCCACCGTCGCATTCTTCTTCTTCTTCCTTGCGCCTCTTTGCTTTTCCTGGAAAAATTAACACACGAAGCTATCGTTTTTGTTAACGGAACCAAACGGGACGTGACTCTAGGCTTCAAGTGCGCAGAAGAAGGTTGTTGCAGAAGCCATGGTTCTTGACACCATTCTGTCTTCTTCTTCTCCTCGTTTAAAGTCTCCATCTTTCCGTAGACCTAAGGATGGACTTGGTAGTTGGTCAACACTTTTCCAGAGGCACCGCTACCTCTTATCTGCTCTCGTGCTCCTTACTATTCTATGTACTGTTTATCTTTACTTCGCCATCACTATAGGAAGTGGCTCCTGTTCTGGGTTGAG
TARGET=275,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2326
PRIMER_LEFT_PENALTY=0.060824
PRIMER_RIGHT_PENALTY=0.171757
PRIMER_LEFT_SEQUENCE=AGAAGAAGGTTGTTGCAGAAGC
PRIMER_RIGHT_SEQUENCE=AAGGAGCACGAGAGCAGATAAG
PRIMER_LEFT=230,22
PRIMER_RIGHT=390,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=60.172
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=0.2878
PRIMER_LEFT_1_PENALTY=0.060824
PRIMER_RIGHT_1_PENALTY=0.226971
PRIMER_LEFT_1_SEQUENCE=AGAAGAAGGTTGTTGCAGAAGC
PRIMER_RIGHT_1_SEQUENCE=CAGAACAGGAGCCACTTCCTAT
PRIMER_LEFT_1=230,22
PRIMER_RIGHT_1=451,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=59.773
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.3731
PRIMER_LEFT_2_PENALTY=0.060824
PRIMER_RIGHT_2_PENALTY=0.312253
PRIMER_LEFT_2_SEQUENCE=AGAAGAAGGTTGTTGCAGAAGC
PRIMER_RIGHT_2_SEQUENCE=AGCACGAGAGCAGATAAGAGGT
PRIMER_LEFT_2=230,22
PRIMER_RIGHT_2=386,22
PRIMER_LEFT_2_TM=60.061
PRIMER_RIGHT_2_TM=59.688
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.3731
PRIMER_LEFT_3_PENALTY=0.060824
PRIMER_RIGHT_3_PENALTY=0.312253
PRIMER_LEFT_3_SEQUENCE=AGAAGAAGGTTGTTGCAGAAGC
PRIMER_RIGHT_3_SEQUENCE=AGTAAGGAGCACGAGAGCAGAT
PRIMER_LEFT_3=230,22
PRIMER_RIGHT_3=393,22
PRIMER_LEFT_3_TM=60.061
PRIMER_RIGHT_3_TM=59.688
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.4027
PRIMER_LEFT_4_PENALTY=0.060824
PRIMER_RIGHT_4_PENALTY=0.341875
PRIMER_LEFT_4_SEQUENCE=AGAAGAAGGTTGTTGCAGAAGC
PRIMER_RIGHT_4_SEQUENCE=GAGAGCAGATAAGAGGTAGCGG
PRIMER_LEFT_4=230,22
PRIMER_RIGHT_4=381,22
PRIMER_LEFT_4_TM=60.061
PRIMER_RIGHT_4_TM=59.658
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_04162 Ps_contig_mira-and-tgicl-ass_franssen_32284
SEQUENCE=TTCTTCCTTTGTCGCCTACCTTTTGCTTTTTCCTTGGAAAAATTAACACACGAAGCTANCGTTTTTGTTTAACGGTAACCAAACCGGGTGACTCTAGGCTTCAAGTGCGCAGAAGAAGGTTGTTGCAGAAGCCATGGTTCTTGACACCATTCTGTCTTCTTCTTCTCCTCGTTTAAAGTCTCCATCTTTCCGTAGACCTAAGGATGGACTTGGTAGTTGGTCAACACTTTTCCAGAGGCACCGCTACCTCTTATCTGCTCTCGTGCTCCTTACTATTCTATGTACTGTTTATCTTTACTTCGCCATCACTATAGGAAGTGGCTCCTGTTCTGGGTTGAGTGGAGCACAGAAAGCTTCATGCCATATGGAGCTTGTCAAGGATTCTATTTCCAATGGCAAACTGAAATTTCTTTAATATTTCAACTATTATTATCGGAGTTTTTGTTGCTATAATCTCTCCACCCCAGTATAGGTGTGACATGAAGTTCAAATATAGTCTTCTGTTACAATATCTGTGATTTGTTAGTGTGTAAGATACAGGCAACAAGCCATTTCTTAGGCACATTATGTTAAGGTTGATGACTGGTTACTATTGTAAAAGTTTGAATTTACCTATTGTTTATGCTAGTCTGAAACCTCTTTTTGAACATTGTCAGCAATAACGGTCTCTTGAATTTTGGGTCAAAGTTCTTTTTACCCAAAAGAAAATAATTGTGTTGATTTTCTTGTTCATTTTATGTGTGATCCATTGAATGTCCACTTAGTGGCTATATTGCATTGGTGAAGTAAGCAATAGCATGAATTGGTGGCATGAGTAACGGAAAAAGGGAAAATGTTACATTTGG
TARGET=155,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2326
PRIMER_LEFT_PENALTY=0.060824
PRIMER_RIGHT_PENALTY=0.171757
PRIMER_LEFT_SEQUENCE=AGAAGAAGGTTGTTGCAGAAGC
PRIMER_RIGHT_SEQUENCE=AAGGAGCACGAGAGCAGATAAG
PRIMER_LEFT=110,22
PRIMER_RIGHT=270,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=60.172
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=0.2527
PRIMER_LEFT_1_PENALTY=0.060824
PRIMER_RIGHT_1_PENALTY=0.191886
PRIMER_LEFT_1_SEQUENCE=AGAAGAAGGTTGTTGCAGAAGC
PRIMER_RIGHT_1_SEQUENCE=GCCATTGGAAATAGAATCCTTG
PRIMER_LEFT_1=110,22
PRIMER_RIGHT_1=396,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=59.808
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.2878
PRIMER_LEFT_2_PENALTY=0.060824
PRIMER_RIGHT_2_PENALTY=0.226971
PRIMER_LEFT_2_SEQUENCE=AGAAGAAGGTTGTTGCAGAAGC
PRIMER_RIGHT_2_SEQUENCE=CAGAACAGGAGCCACTTCCTAT
PRIMER_LEFT_2=110,22
PRIMER_RIGHT_2=331,22
PRIMER_LEFT_2_TM=60.061
PRIMER_RIGHT_2_TM=59.773
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.2982
PRIMER_LEFT_3_PENALTY=0.126400
PRIMER_RIGHT_3_PENALTY=0.171757
PRIMER_LEFT_3_SEQUENCE=GCCTACCTTTTGCTTTTTCCTT
PRIMER_RIGHT_3_SEQUENCE=AAGGAGCACGAGAGCAGATAAG
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=270,22
PRIMER_LEFT_3_TM=60.126
PRIMER_RIGHT_3_TM=60.172
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=0.3731
PRIMER_LEFT_4_PENALTY=0.060824
PRIMER_RIGHT_4_PENALTY=0.312253
PRIMER_LEFT_4_SEQUENCE=AGAAGAAGGTTGTTGCAGAAGC
PRIMER_RIGHT_4_SEQUENCE=AGCACGAGAGCAGATAAGAGGT
PRIMER_LEFT_4=110,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=60.061
PRIMER_RIGHT_4_TM=59.688
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_04163 Ps_contig_mira-and-tgicl-ass_franssen_32288
SEQUENCE=AAGGGACGTTAATAATAAGTAGATACTAAGAAAAACTTACTAAGAAAGAAAGACGGAAAGTACAGATCCGAGTGCACAGGAAGGGTACGTTCTGCTGAAAATAGAGGAGGATGCAGATTCGCGCTATCGATTCTATGAAAGTGCGTCTGCAAGAAAAAGAACTGTGACAGCAGAAGAAAGAGAAAGGGCAATTAATGAATCAAAAACATTTGAACCAACTAATCCTTTCTGCCGAGTTGTGCTGCGACCCTCTTATTTATATAGGGGATGTATAATGTACTTGCCTTCCTCTTTTGCAGAAAACAATTTGAACGAGGTTTCAGGGTTCATTAAACTTCTGGGCCCGACCGGGAAAGAATTTCCTGTCCGCTGCCTATATAGAGGTGGCCGAGCAAAGTTAAGCCAAGGATGGTATGAATTTACAGTAGAGAACAGTTTAGGGGAAGGCGATGTCTGTGTTTTTGAGCTGCTTAGAAGCAGAGAGGTAGTGCTGAAAGTTACTTTGTTTCGTGTATGCGAGGATGAGTCAGGGTTATTCGACCCTTCTACACAGGTGAGCCATAACGTGAGCCATGCAAAACTGCTGAACCCTCACTTGCAGCACCGTGTCAGCACCATGAAACCGGTTAAATATTAGCAGGGAGAGTTAACTCCTCCTGATAGACTATACAATTCTGTCCAGTAGTTTTTACTTCTTCTGAGTGATCCTGGAATTCTCTGGTTTATTCAGTTTGGAACTTTGATGATTTCCATGTCCATAGCCATATTTGAACTTAGTTATGGTAGTTTTGATGTGGATATTTTGTTTTCGACTTGGATTGTAAATAGACTGTTCAATCTATTCAATCATAAACTGAGTCGAAACGCTTGTCCTAGCGCTTTTCTTTTGCTGTTGAATAGAACGCATGTCCTGGTGCAGACTAGTTTTGGGTTTTAACTTTTTTGNTGGATCTTGTTTGTAGTTTCATCTGCATGAATATAATGACTCAGTTACTTTAAGTTTT
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04164 Ps_contig_mira-and-tgicl-ass_franssen_32289
SEQUENCE=GAAGTGACATGCAAATTTAACATTNTCGNGNGGGGGTTTTCGTGGCTGATACAGAGTATTGCCACTGCATAGGTTCAAATCTTCAATCAATCAAAGTTGAGATGAAAACAATGGCTTCACCTTCTCTTCAATTTCATCTTCTTCCATCTCTCTCCAAATTCTGGTCTTCAATATCCCCTCTTTCCCAACATCACACCCATCCATTCTCTTCCACAACCTCTCTCAGATTCACTTCCACCAATTCCACTACCACTTTTACTCCCAAACCTCTCTCTCCGTGTTTCGCTCTAACAGAATCCAACTCGCCTAATTCCACACAACCCGACCCAAAAACTCTTCTCCAAGACATTGCCGAAAGTTTTGATCTTCCTTCTGATTACTTTGCAAAGTTTCCCAAAGATCTTCGATTAGATCTAAATGATGCTGCGTTCGACCTTTCAAATGGACCGATCTTGGATGAGTGTGGCAAAGAGTTAGGAGAGACATTGCTAAACCTCTCTCGAGCATGGGAGGTTGCTGATACATCATCCTCTCGCTCTTTAGTGGCAAAGCTTCCTTTGATTGAGGCTAGTTTGACAGGCACTGCCAAGTCAGCACTTGGAAAACGTTTGGTAGCCGCTGGAAGAAGGTTTCAGTCAATGGGACAATATGGTCAAGATTGCAAAGGCAATGATTGCGGCTGGGAGAGCTTTATCTGCTTATTCAACATCTGCAGTTATTGATAAACAACCCAAGGAGGAGACTAAGACGCTGAAGTTTGGAGCATTGCAAGTTGAAATTACTCCCACTAAAGCCAATATAGGGGCTGCAATTGCATTAGTTTTTGGGATTCTTACATGGGAGATAGCTCAGGGAGTTCAAAGCACTCCAGAGAATTCTTTGCAGTATGCAAATGACAATGCGTTGATGCTTGCTAAGTCTTTGAGGGGAACACTGCTTGCTCTTTTTTACGCCTCCACGTTCTTGTCTGCACTTACTTCGGCAGGGCTTGTTTTACTTGGTATACAACTTAAATCAGAGAAAAATTGAGCTTTGTGTGTGCTTCCTTCGCTCGGTTTTGCTTGCAAATGCTGGAGAAGCTGCACAGTGTAAGTTTGTGAGTATTAGTATACAATCATAATACCAAACTCGTGACACGGTTTGAAAATATTGTCCTAACCATCCAGATTGAGTCAGGTTTCTTTCATCTATCCTGCCAATTTTCTAATGTGTAAACTTTATATCATGTAAATAGACACATTCAGTTTTAAACTTCTTATGGATGGAGTGATGTGTGTAACTTCT
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1072
PRIMER_LEFT_PENALTY=0.024665
PRIMER_RIGHT_PENALTY=0.082508
PRIMER_LEFT_SEQUENCE=GTATTGCCACTGCATAGGTTCA
PRIMER_RIGHT_SEQUENCE=ATGTTGGGAAAGAGGGGATATT
PRIMER_LEFT=55,22
PRIMER_RIGHT=190,22
PRIMER_LEFT_TM=60.025
PRIMER_RIGHT_TM=59.917
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=0.1201
PRIMER_LEFT_1_PENALTY=0.024665
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=GTATTGCCACTGCATAGGTTCA
PRIMER_RIGHT_1_SEQUENCE=GAATTAGGCGAGTTGGATTCTG
PRIMER_LEFT_1=55,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=60.025
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.1348
PRIMER_LEFT_2_PENALTY=0.024665
PRIMER_RIGHT_2_PENALTY=0.110131
PRIMER_LEFT_2_SEQUENCE=GTATTGCCACTGCATAGGTTCA
PRIMER_RIGHT_2_SEQUENCE=GAGAGGTTGTGGAAGAGAATGG
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=221,22
PRIMER_LEFT_2_TM=60.025
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.1348
PRIMER_LEFT_3_PENALTY=0.024665
PRIMER_RIGHT_3_PENALTY=0.110131
PRIMER_LEFT_3_SEQUENCE=GTATTGCCACTGCATAGGTTCA
PRIMER_RIGHT_3_SEQUENCE=AGAGGTTGTGGAAGAGAATGGA
PRIMER_LEFT_3=55,22
PRIMER_RIGHT_3=220,22
PRIMER_LEFT_3_TM=60.025
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.1419
PRIMER_LEFT_4_PENALTY=0.024665
PRIMER_RIGHT_4_PENALTY=0.117281
PRIMER_LEFT_4_SEQUENCE=GTATTGCCACTGCATAGGTTCA
PRIMER_RIGHT_4_SEQUENCE=TGGATTCTGTTAGAGCGAAACA
PRIMER_LEFT_4=55,22
PRIMER_RIGHT_4=299,22
PRIMER_LEFT_4_TM=60.025
PRIMER_RIGHT_4_TM=59.883
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_04165 Ps_contig_mira-and-tgicl-ass_franssen_32307
SEQUENCE=CATAAAATTTATTTCACTTTAAAGATATCTCAAATCTTACATCCTAAGAATGAAATTTCCGTATAAGAATTTGAATTATACTCTAAAATTCCATTTCCTCAAAAAATTCTCTTAAATTTAAATTATCCTCTAAAATTATATCACCTCAAAAATTTAAATTACCCTCTTATTCAAAACATACTCTGATAGTTTTTCTCTTTTCAACATAAAAAACAAGAAAGTCAGCAGACATATCCAAATACCTAGCCTATGAAGAGTATAACTTGAGAAGTTCTTCCTGAAAAGTCTTTCTGACGGCCTCTCTCTTGAGCTTGAGAGCAGCAGTGACTAGGCCAGACTCGGGTGTCCATGGATCAGAAAGCAATTTTATTTTTGCTGGAATCTCAAGTTTTTGCAAACCAGCCTTCTTTGCCTCCTTTACTAGTGACGCGTGCACCTCTTTCAAGCTTTCTTCTTTAGAGCAGAGTTCTGAAAAACCAGAATAAGGAATTCCTTGCTTTGAAGCCCATTCTTCCAATGTTGACTGAGAAGCCACAACAAGTGCCACACAGTAACTATGGAAAGGATCAGCATGCAACATAATATTGTCCACATAGGGGCTGACAATAAGAGCTGCCTCAACCTTTCCCAAAGAGACATACTCTCCATGCTGCAGTTTAACAATGTCCTTTTTACGATCAATGATCTCAAGACAACCATCTAGATGAAACCTCCCTATGTCACCAGTGTAGAACCATCTCATTCCTTTTTCATCAACCTTGTATGATTCTTTTGTCTTTACTTCATTCTTGAAGTACCCAAGAGTGACATTTGGACCGCCGATTACAATTTCACCCCGGGGCATTGGTGAGTCATTAATCAAATACCCACCTTCGGGCCAGTCGATAAGCTTAATGTATGAGCAAGGAAGAGGTGGTCCAACACGGCCGACGGATGTATCATCAAAATCAGAGAATGTCCCACCAGCACAAGTCTCTGTAAGACCATATCCTTGACCTATTGGAGCACCGAGGCAGATATTGATGAACTGTTGAGTATCACCAGAAAGGGGAGCACCACCAGACAGTATAAAACGGATACGGCCGCCTAGAATTGCTCGGACCTTTTTAAACACAAGAAAGTTCCAGAGAGCCTTTTCCAAGCCCCAAGCTCCAAACCAGCTCCCACTAATAGCTTGCAACCTCCTCTCATAGGATAAATAAAACAACTTCTTTGATAATCCTCCTGTTGTATTTACCTTCTTAAACACTCCATCGCGAACACGGTCAAGAATTGCAGGTACAGCAGCCATTAAAGTTGGCATCAACACTGTAGCATCCCCCTTTGTTCCTTTCTTTATCTTGCTTGATGTATCTGTTAGGGTCAAAGGAGATCCATATCCTATAGCAGCCCCAACAGCTGCCATCAAATTCTCGGCTGCTAGTTCAAGGATATGAGCCATTGGCAAGTATGCTAGATATATATCCTTTTTCCCAAGTTTGGGAACAATTGTCTTCACAGCCGAGACTGTAGCTAAAACATTGCCATGTGTCATCATCACACCCTTGGGTAATCCGGTACTTCCACTTGTATACATAATAACTGCAACATCTGGTGGGAGAGGTAAATCAGCATCAACAGGGTTTTCTTTACCAAGTCTCTTAACATCTGCAAAGGATATGACTGTCCAGCCATGTCCAACAGATGAGGCATCAGATGGTATCTCATCATCCAAGCATATCACACGTTTCACCGAGTCAAGTTGTCCACTAATATGTACAAGTGTTTTCAATTCTTTTTTCCCGCAAATCACTGTTGTAGCCTCTGTCTCATTTAGGGAGTGACATAAAGCTTCCTCTCCCRGTGATGCATACATAGTTACTACTGTCACATTTCGCCTGAAACAACCCTGCAGAGCAAGGAACCACTCTTCCCTTGTATCAGCAAAGATAGCCGCACGCTGTTCCCTTCCATGTCCCAAAGCAGCCAATCCACTCCCAAAGCTAGAAACAGCTTCAAACGCTTCAGCATACGATAGCCACTCATAACCTCCCAATTCAACCTTCTCGAACTGTCTCCCGTCGTTACTCGTTTCCAATTCCTTCGATATAAGCAACCGCGTCCCAAGCAACAGTCCCTCCCCGTACTTCCTGCACGCTTCTTCGAATAGCTCCGCCAGCGTGCTCACTCCCTCCCATACCGTCTCCACCGGCGAATCGAAGCGCTTGTTTCTAACGGTTACGCCGGGATCGCCGGTTGTGTCGGTCGGAACTCCACGGCGTTTGGGGTGGTTGGAGCGGAAGAGGAGAGTGGCGAGAACGGGGAGTATGACGCCGAAAATGTAGGGATTCATCGATGAGTTGGTTACGGTGGTTGTGAGAATCGAGGAGGAGGAGGAGGAGGTTATTTATTGTTATAGAGTTTTACAGCTCACTCACAGTTGGCTCTGTCTTGTTGTTTTACTTCACTTCCTCATTTCATATTTTATCTTTACTCTTGAAATGATTGGTTACACTT
TARGET=2364,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04166 Ps_contig_mira-and-tgicl-ass_franssen_32315
SEQUENCE=AGGTTNTTTTNACCTTNAAGAAATATCAAGATATATATATATATATGTTATTGCCATAGTGAGTTTTTCCAAGACAGAAAACTACTGCATTACATAGTGATGATGATGATACTCAGCAAGATCTGTTTGCACCATCATCTTCTTCTAGCCTCCTACTACCTCACTTTCCACACACATACACCATTATAAACACAAACACGCCCTACTATAACTACAAATTTGAGGTAACTTGCAGGACACTCAATTTATACATTAACAACCTACCCCAAAAGGATACATAAAGCAAGCTGTTCGATCAAGTGCACTGTCTATTATGTTACTTTATCCCTCGTCTTGAATATATTCGACTAAATCATTAGGTCAGCGTATGAGTAATTATGACGTCGGCCATATTCAAGAAGGCTGCCATATGTACGATCCCAAACCCCGATGAAAAGGGATTCGGTGTCTGGACTGAAAGATATGCCGGATATCTCACCGAAGAAATCGATTTCCTGTTCTTTCTCATACCCACTTTTCACATCATATACATGGACAAAATCTGCAGGCTCGGCTATAGCCATATACTTGCCGTCAGAAGAATGTCGGATTGAACGGATAGCTCCGAGATTTCCCTTCAACACTGCCACTGACTTGGATAAGTTTCGCATGTCCCAAATCCTACAGGTTTTGTCCTGGTTTCCAGTAGCAAAGGTCACGCCATCAGGATTCCATGATGATGCAAATGAAAAATCTAAGTGCCCAGATAATGGAGCAATAGTCTTTCCATTTTGAGAGTCCACCAACATGCTTTCAGGATTGTCTCCAACAATCAGAAGTAGCTTACCATCTGGACTGAGAGAAGTATGATTCACTGGCCAGGGAAAACGAAAATGCTTAGAGAGTTGAAACTTCTCCATATCAAAGTTTCTGATTCCACAATCATTATTTGATGCTGTGAAATGAACTGCCCCACTAGGAGAAGCATAAATCTCAATGGCATTGGTGATAGCGTTATCATCATAAGTAGTCCTTGAGCAAAAGCTGACACCAGGTCGGTCTAGGTGCTTGCAAATAAGTTCACCCTGAAATCCTCCAGCAACTAGAAGATTATCTTTTACTGCAAGTGTACTAACTTGAGTGTGTGTGAATCCTTCCAATAGACTTCCAGGATGTTTCTCTGATGGTGCAACATGTCCTGAAACATTGAGTACTTCAGACCTTGTGCAAGTCAAAGAAGACCAATGTGTTACTGAGAACTGAGACATAAGGTATACATCATGCTTTGATGTAGCCCAAACCAAATTCCTCAACTGAAAATGAAGAATTGTTGATTTTACTGATCTTGAATTTCTCCTGAACTCATAAAATGAGTATCCTTTGTCTGTACTGTTACAATCCTTCCCTGACCTTTCCCCTGATTGGGGGATATTTTCATAATTCTTGTATTGCTCAAGCCTCGTTTGCCGATACCTTTCTCTGGTGATGCTAAGGCTATCCCACGGAATCCCCTGGATGTCTTGTCCTCTTCTAGCTTGAGCAGCTGTAGTGTCTACCGCTTTACTACTCGAGTAATCATATTCATCAACGTCAGAGTCAACGTCACCTCCCTCTCTATTAATAGATTCACCATCTATATCGATATCCTCTTCTACATCATCCATTTCATATTCATCCGCAAAATATTCAGCATCACCTCCTTGGCGATGAGACATGTTTTTGACCAATTAGAACCTTTCTTTGTCGGAAGGAGCTTCTTCGATCTAATAATTTCAGAAATCCTC
TARGET=31,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04167 Ps_contig_mira-and-tgicl-ass_franssen_32315
SEQUENCE=AGGTTNTTTTNACCTTNAAGAAATATCAAGATATATATATATATATGTTATTGCCATAGTGAGTTTTTCCAAGACAGAAAACTACTGCATTACATAGTGATGATGATGATACTCAGCAAGATCTGTTTGCACCATCATCTTCTTCTAGCCTCCTACTACCTCACTTTCCACACACATACACCATTATAAACACAAACACGCCCTACTATAACTACAAATTTGAGGTAACTTGCAGGACACTCAATTTATACATTAACAACCTACCCCAAAAGGATACATAAAGCAAGCTGTTCGATCAAGTGCACTGTCTATTATGTTACTTTATCCCTCGTCTTGAATATATTCGACTAAATCATTAGGTCAGCGTATGAGTAATTATGACGTCGGCCATATTCAAGAAGGCTGCCATATGTACGATCCCAAACCCCGATGAAAAGGGATTCGGTGTCTGGACTGAAAGATATGCCGGATATCTCACCGAAGAAATCGATTTCCTGTTCTTTCTCATACCCACTTTTCACATCATATACATGGACAAAATCTGCAGGCTCGGCTATAGCCATATACTTGCCGTCAGAAGAATGTCGGATTGAACGGATAGCTCCGAGATTTCCCTTCAACACTGCCACTGACTTGGATAAGTTTCGCATGTCCCAAATCCTACAGGTTTTGTCCTGGTTTCCAGTAGCAAAGGTCACGCCATCAGGATTCCATGATGATGCAAATGAAAAATCTAAGTGCCCAGATAATGGAGCAATAGTCTTTCCATTTTGAGAGTCCACCAACATGCTTTCAGGATTGTCTCCAACAATCAGAAGTAGCTTACCATCTGGACTGAGAGAAGTATGATTCACTGGCCAGGGAAAACGAAAATGCTTAGAGAGTTGAAACTTCTCCATATCAAAGTTTCTGATTCCACAATCATTATTTGATGCTGTGAAATGAACTGCCCCACTAGGAGAAGCATAAATCTCAATGGCATTGGTGATAGCGTTATCATCATAAGTAGTCCTTGAGCAAAAGCTGACACCAGGTCGGTCTAGGTGCTTGCAAATAAGTTCACCCTGAAATCCTCCAGCAACTAGAAGATTATCTTTTACTGCAAGTGTACTAACTTGAGTGTGTGTGAATCCTTCCAATAGACTTCCAGGATGTTTCTCTGATGGTGCAACATGTCCTGAAACATTGAGTACTTCAGACCTTGTGCAAGTCAAAGAAGACCAATGTGTTACTGAGAACTGAGACATAAGGTATACATCATGCTTTGATGTAGCCCAAACCAAATTCCTCAACTGAAAATGAAGAATTGTTGATTTTACTGATCTTGAATTTCTCCTGAACTCATAAAATGAGTATCCTTTGTCTGTACTGTTACAATCCTTCCCTGACCTTTCCCCTGATTGGGGGATATTTTCATAATTCTTGTATTGCTCAAGCCTCGTTTGCCGATACCTTTCTCTGGTGATGCTAAGGCTATCCCACGGAATCCCCTGGATGTCTTGTCCTCTTCTAGCTTGAGCAGCTGTAGTGTCTACCGCTTTACTACTCGAGTAATCATATTCATCAACGTCAGAGTCAACGTCACCTCCCTCTCTATTAATAGATTCACCATCTATATCGATATCCTCTTCTACATCATCCATTTCATATTCATCCGCAAAATATTCAGCATCACCTCCTTGGCGATGAGACATGTTTTTGACCAATTAGAACCTTTCTTTGTCGGAAGGAGCTTCTTCGATCTAATAATTTCAGAAATCCTC
TARGET=98,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8352
PRIMER_LEFT_PENALTY=0.733091
PRIMER_RIGHT_PENALTY=0.102139
PRIMER_LEFT_SEQUENCE=TGCCATAGTGAGTTTTTCCAAG
PRIMER_RIGHT_SEQUENCE=TGTATCCTTTTGGGGTAGGTTG
PRIMER_LEFT=51,22
PRIMER_RIGHT=277,22
PRIMER_LEFT_TM=59.267
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.9266
PRIMER_LEFT_1_PENALTY=0.733091
PRIMER_RIGHT_1_PENALTY=0.193502
PRIMER_LEFT_1_SEQUENCE=TGCCATAGTGAGTTTTTCCAAG
PRIMER_RIGHT_1_SEQUENCE=TGCTTTATGTATCCTTTTGGGG
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=284,22
PRIMER_LEFT_1_TM=59.267
PRIMER_RIGHT_1_TM=60.194
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=1.0643
PRIMER_LEFT_2_PENALTY=0.733091
PRIMER_RIGHT_2_PENALTY=0.331254
PRIMER_LEFT_2_SEQUENCE=TGCCATAGTGAGTTTTTCCAAG
PRIMER_RIGHT_2_SEQUENCE=CTTGATCGAACAGCTTGCTTTA
PRIMER_LEFT_2=51,22
PRIMER_RIGHT_2=299,22
PRIMER_LEFT_2_TM=59.267
PRIMER_RIGHT_2_TM=59.669
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=1.1298
PRIMER_LEFT_3_PENALTY=0.733091
PRIMER_RIGHT_3_PENALTY=0.396675
PRIMER_LEFT_3_SEQUENCE=TGCCATAGTGAGTTTTTCCAAG
PRIMER_RIGHT_3_SEQUENCE=TGATCGAACAGCTTGCTTTATG
PRIMER_LEFT_3=51,22
PRIMER_RIGHT_3=297,22
PRIMER_LEFT_3_TM=59.267
PRIMER_RIGHT_3_TM=60.397
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=1.3324
PRIMER_LEFT_4_PENALTY=0.733091
PRIMER_RIGHT_4_PENALTY=0.599276
PRIMER_LEFT_4_SEQUENCE=TGCCATAGTGAGTTTTTCCAAG
PRIMER_RIGHT_4_SEQUENCE=GAGTGTCCTGCAAGTTACCTCA
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=241,22
PRIMER_LEFT_4_TM=59.267
PRIMER_RIGHT_4_TM=59.401
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_04168 Ps_contig_mira-and-tgicl-ass_franssen_32319
SEQUENCE=GGGGTGAATTTTGTAACTAAACTAGYTAGAAAAAGAAACATGTAACAATAATTGATCTTCCAAATCAAGATTACATTTCATATAGATGGTAACAATTACATTAATAGGCCACTCTCAATAAAGGCAAAACAAAAGCCTAAAAGGAGTGATGATAATAATAATAGCACATGGAACCCATAAACTCAAAAGGCTATCTCAAAGTACATCAACACATTTTTTAATAAACCCAAAAAGTGTTACCATCTAGTTAGCATATAATAATAAATTATAATATAATAAAGGACCATATAGATAAATTAATAAAAACTTTATATAGTTGATTCATTGATGAAGCTTTTATTTTAAGGGCATTTTGGTCCTCCTTGTTGGGTCTTCCAGTTGTTGTAGCAAGGGCACACTGCTTTATTACCATAATACCCTGGTGGTACACACAAGCATTTCTTGCAACACTTCTGACAGAAAAACATGCATGGCTTGTGGTATTGTGTCTGTCCACATCTCCTCGAACATTGTGAAGGGCATTGGTAGCTCTTAAGACTCCCAGGTCCATATTTGCTCTTGTTATCATAGAGATGGCCTCCTTGGTCATTACCAGCCATGACCAATGTTTGGATCATGGAAATGGCAATGAGAGCTAAGATGAGAGAAGCAAAGAACTTGGTAATGGCCATTGTAGCACCTCTAGATTTGGCAAAAATGTTTGAAGAGAAGAGAAAGATATTAATGGAGGAGATGGTCCCCGCGTACTCTGCGTTGTATTACCTACTGCTT
TARGET=152,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04169 Ps_contig_mira-and-tgicl-ass_franssen_32321
SEQUENCE=AAAAAATATAATAATTATTTCACAAAAACAAAAAACAAAGAAAAATAGAGAGAAGGTTCTTTTTTTTTTATAATCTTTTTTGAAAATGAGAGGCATGTGATGTGATGCAAATTGCAAATCAAGCGGAATCCTCGGGTAGTTCGTTATTGAGAGGATTTTCCTTTTTTACCCCTGACTGAATTCCGTAATAACGAACGTACCACTTTACGAATTTTCTCAAACCCGTTGAAAGATCCGTTGTTGGTTTGTACCCGAAATCTGTGTAAGCTAATGTCACATTCGCGTGCGTATACGGAACGTCGCCGTTTCGTGGCATCTTGAGGATGTGTTTCTTCGCCTTCGTGCTCAGCAAGTTTTCTAGAATCGTTACAAGTTTCCCCACCGGCACCGGCGATGTGTTGCCGAGATTGTAGATTCTTAATTGCGCCGGTGCCTTCTTCTTTCCGCCGCTTCCGGTGCTTTTCTCCGCTGTGTCCAAAGCTCCTACGCAGCCTTTCACGATATCGTCGATGTAGGTGAAATCACGCGCGACTTCCTTCCCTTCCTGCGTCTGGTAAACATCGATGGTTTTTCCATGAAGAATATCTTTGGTGAAGAAGAAATACGCCATGTCTGGTCTTCCCCAAGGACCGTACACCGTGAAGAAACGGAGTCCGGTGAGGGAGAGACCGTAGATGTGATTATAAGTATGAGCGATTTCCTCGCCGGCTTTTTTAGTTGCGGCGTAGAGACTCGCCGGTTGATCTGTCCGGTGAAGTTCTGAGAATGGATTCTCCGTGTTGAGACCGTAAACTGAGCTTGATGAAGCCCAGACGATCGCCGGTTGAGGATTGGCTGCTTTGGAAACTTCTAGAAGATTGACGAAACCAGCAATGTTGGATTTGATGTAGGATTGTGGATTTTGCATGGCGTAACGAACACCGGCTTGTGCAGCGAGATGAAGGATGTGAGTAATCGGAACTACATCGAAGAGTTTGGTTAACAACGGCGCGTCGTTTAGGTCACCTTCAACAATGAAGATTTGATGCTGAGTTAATAAAGCTTGTCTCGCGCGCTTCAACGACGGGTCGTAATAGGTGTTGAAATTATCTAAACCTAAAACTCCGTCACCTCGTTTTTTAAGAGCGAGTGAACAGTGAGAACCGACGAAACCGGCTGCACCGGTTACAAGTACTGTGAAACCATTAGGACGGCGCGGTGTAGCGGAGTGACGGACTTGGCGTTCCCATGCACCGCCGCCGCCGAATGCGGAGGTGAGAAAGTGGGAATGAGTGTGGAAGTGGTGGTTGGTGGAGTCGGAGAGTGGAGGGTAATTGAAAGTGAAGAAGAAAACGAGGACGAGTGCTATCAAGAGTGTGGCTCGGAAGAGAAGTTTGGAAGATGCGTTAAGGAGTTTTGTGCTGTTAACTTTTCTGATGTAGCTATTGTAACGTTCTAGCTTTACGGTTTTGCTTGTGTCTGGTGGAGAAGCCATTTTGCTGTATAGGCAAAAAAGCTGCAAAAATGGAATAGGATTGATTTCGATTTGGGGTTTGTGATTAAAGAAAGAAATAGGTGATGATGGGTGTCAGTGTTTATTGCGTTGGAGTGAGGAAATTGTATTGTTTTTTATGATAAGGAAAAAAGGGTTACCTCTAAATGTGTGAGAGCTTGATTTGAGGTTGAGCCCTCAAGCTTGAAGGTGTGAGAGGATTATTAATATAAAGGGAAGGAGAGAGGAAAGAGAGAGTGGGATGGGTTGGTGTTGGTTGGAGACCACTTTCTTTTTCTACTTAACCCTTCACACCATCATAAACACTCATGGAAAGTGCTAGTAGTAATAGTAATAATGATATTTAAGTTAATGTTAACTTAGTACTTAAATTTAAATTTTCTTCTTTTCCTTATTTTAGTAAAA
TARGET=1233,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7201
PRIMER_LEFT_PENALTY=0.642030
PRIMER_RIGHT_PENALTY=0.078029
PRIMER_LEFT_SEQUENCE=ACGGGTCGTAATAGGTGTTGAA
PRIMER_RIGHT_SEQUENCE=CACTCGTCCTCGTTTTCTTCTT
PRIMER_LEFT=1062,22
PRIMER_RIGHT=1343,22
PRIMER_LEFT_TM=60.642
PRIMER_RIGHT_TM=59.922
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.7201
PRIMER_LEFT_1_PENALTY=0.642030
PRIMER_RIGHT_1_PENALTY=0.078029
PRIMER_LEFT_1_SEQUENCE=ACGGGTCGTAATAGGTGTTGAA
PRIMER_RIGHT_1_SEQUENCE=CGTCCTCGTTTTCTTCTTCACT
PRIMER_LEFT_1=1062,22
PRIMER_RIGHT_1=1339,22
PRIMER_LEFT_1_TM=60.642
PRIMER_RIGHT_1_TM=59.922
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.7278
PRIMER_LEFT_2_PENALTY=0.718884
PRIMER_RIGHT_2_PENALTY=0.008930
PRIMER_LEFT_2_SEQUENCE=AAGAGCGAGTGAACAGTGAGAA
PRIMER_RIGHT_2_SEQUENCE=TCTTCCAAACTTCTCTTCCGAG
PRIMER_LEFT_2=1120,22
PRIMER_RIGHT_2=1380,22
PRIMER_LEFT_2_TM=59.281
PRIMER_RIGHT_2_TM=59.991
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.7905
PRIMER_LEFT_3_PENALTY=0.642030
PRIMER_RIGHT_3_PENALTY=0.148509
PRIMER_LEFT_3_SEQUENCE=ACGGGTCGTAATAGGTGTTGAA
PRIMER_RIGHT_3_SEQUENCE=CACACTCATTCCCACTTTCTCA
PRIMER_LEFT_3=1062,22
PRIMER_RIGHT_3=1275,22
PRIMER_LEFT_3_TM=60.642
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.7969
PRIMER_LEFT_4_PENALTY=0.718884
PRIMER_RIGHT_4_PENALTY=0.078029
PRIMER_LEFT_4_SEQUENCE=AAGAGCGAGTGAACAGTGAGAA
PRIMER_RIGHT_4_SEQUENCE=CACTCGTCCTCGTTTTCTTCTT
PRIMER_LEFT_4=1120,22
PRIMER_RIGHT_4=1343,22
PRIMER_LEFT_4_TM=59.281
PRIMER_RIGHT_4_TM=59.922
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_04170 Ps_contig_mira-and-tgicl-ass_franssen_32325
SEQUENCE=AAAAAAAATTAAAATCNAATTTTATTTAATTCCCGTCACTACACTACTTGAATATGAAAAAACAAGTCATATTTGTTACTAGTTCTATCTGCGCAGTTTAAAAGCTGCATCTAGCATCCATAGAAAGAAGAATACAAAGTTGACGAACTAAAATGCACAAGTGGGTGATCCCCTTCGAAAATCCATTATGACAAGTGTGGCAGTGCTATGTGCAAGTGCCCCAAGAACAACAAAAACATGAAATATCTGATGACTATGTCCTGCAATATCAAATGCTCCAGGCTTCCATCTCTCTGGTATTCTAGTCACATAAAACACAACACCTATGGCATATAGAATCCCCGTTATCAGCTCATATCCAATTGCAACTACAATATGCGACTGACCCCAGTTGCTTACAAGAGCATGAACTGCTGGAATAACACCTGAAAATCCCATTGAAAGGAACAATGATGCTCTGAAGGTTCGAAACCGAGGCGCTGAAAGTGTTGGGGAAAGAAGGGTTATTATTGCCACTACTCCAAGGACTGATATTGATGTCAAATAGAGTAACCTTGCATATGGATTGCAGAAAAAGACGTAGTAAATTGGAGCATAGAAAGAGGATACTATCATGATTGATATTCCGGCGTAATCTAGACGCCAGAAAAAGAGGTTGAAGCGTTTGGAGTGGCAAGCTAGAAGATGGGAAATAGAGCTGCAAGCTAAGCAACACATTCCTCCAACTAAGAAAATGAACCATGGCCATCTTGGAATTGTTTCTGTCACTCCATCAAAGATGGTTACTTCCAATATCTGTCTCAAATGCCAATCCTGAAAGCCTTTGTTTTGAGATTCAGAACCGTTGATCTGCTTTCCCATGGCTCCTACTCTGTAAATTTTAGAGAGAAAACCTCCTCCTCCTACGAGTTCGGTCGCCGCCGGAAAACTCACCACCGCCATCGCGGCAAATATGAAAAACCCTCCTAAATGAGTCCAAACATTCAGCGTCTCGTTATGCCACGCGAAAACGCTCAACAAAGAATCCTTCACCGGCCATTCCGACCGGTAATGATCCAAAATGAACTCATTATCTTTCAAATATTCCGGTAATTCTTCAAACTTCACCAATCTCGACTTCGTTCTCTTCCGCTTATCGCTTTTCTGCCGATGAACCTTCATCTCAGCGAGCTCGCCGGAAAATCTTATCTTCGTTTGTTTTCCGGCGAGAATGTTACCAAATATAAATATTTATCTATCTATTCCTCCGGTTAGTTACTGGCCTGTCNCTATTTATACCCAAACAAACAGT
TARGET=893,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1475
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.115769
PRIMER_LEFT_SEQUENCE=ATCTGTCTCAAATGCCAATCCT
PRIMER_RIGHT_SEQUENCE=GGATTCTTTGTTGAGCGTTTTC
PRIMER_LEFT=793,22
PRIMER_RIGHT=1026,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.116
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.1559
PRIMER_LEFT_1_PENALTY=0.040146
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=CCAATATCTGTCTCAAATGCCA
PRIMER_RIGHT_1_SEQUENCE=GGATTCTTTGTTGAGCGTTTTC
PRIMER_LEFT_1=788,22
PRIMER_RIGHT_1=1026,22
PRIMER_LEFT_1_TM=59.960
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.1567
PRIMER_LEFT_2_PENALTY=0.040146
PRIMER_RIGHT_2_PENALTY=0.116582
PRIMER_LEFT_2_SEQUENCE=CCAATATCTGTCTCAAATGCCA
PRIMER_RIGHT_2_SEQUENCE=GGTGAAGGATTCTTTGTTGAGC
PRIMER_LEFT_2=788,22
PRIMER_RIGHT_2=1032,22
PRIMER_LEFT_2_TM=59.960
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.2520
PRIMER_LEFT_3_PENALTY=0.137489
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=ATTCAGAACCGTTGATCTGCTT
PRIMER_RIGHT_3_SEQUENCE=AAGAGAACGAAGTCGAGATTGG
PRIMER_LEFT_3=833,22
PRIMER_RIGHT_3=1127,22
PRIMER_LEFT_3_TM=60.137
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=0.2533
PRIMER_LEFT_4_PENALTY=0.137489
PRIMER_RIGHT_4_PENALTY=0.115769
PRIMER_LEFT_4_SEQUENCE=ATTCAGAACCGTTGATCTGCTT
PRIMER_RIGHT_4_SEQUENCE=GGATTCTTTGTTGAGCGTTTTC
PRIMER_LEFT_4=833,22
PRIMER_RIGHT_4=1026,22
PRIMER_LEFT_4_TM=60.137
PRIMER_RIGHT_4_TM=60.116
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_04171 Ps_contig_mira-and-tgicl-ass_franssen_32336
SEQUENCE=TTCAACAGAATTCATTCTACTCCAAGACCACAACTTGTCATAGTTCAAAGAATTGTTATTCAAAAAAGGGTCAAAAACCTTACTTGGCCTAACCTCCCAGGCAATCTCATGAAGCAAAGAAACACACGAATCACGATCTTCAACAACAACAACATCATCAACAACAGAAGATGATTTAACCGATGTAATGAAATCAGAAACAGAAGGGAAATAAGGAGTTAGATGAACACCAACAGCAAGAGTAATAAAAAGTGCACCACTAAAAAGGAGAACTTTGTTACGACTCAAGTGCCAACCAGCCATACCCATTGGAACAAACAACACAACACATGCTGCAAATAACCCCAACTGAACAGCACCAAACATTGTTCGAATTTTCCCTTCTCAAAATTCACATCTACACAAATTTAAAAACGGGTTTTTTAGGGTGTTGTATTTTTAGCCTGAGATTCTAGAAAGAAAAATTGTAAACTTTTCTTAGACCCGAAGGAAAAAAGGGTGACAGTAGGTAAAGGTTGGGGTGGGGCGTT
TARGET=141,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1665
PRIMER_LEFT_PENALTY=0.099075
PRIMER_RIGHT_PENALTY=0.067430
PRIMER_LEFT_SEQUENCE=AACCTTACTTGGCCTAACCTCC
PRIMER_RIGHT_SEQUENCE=GGTTATTTGCAGCATGTGTTGT
PRIMER_LEFT=75,22
PRIMER_RIGHT=343,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=269
PRIMER_PAIR_PENALTY_1=0.1731
PRIMER_LEFT_1_PENALTY=0.099075
PRIMER_RIGHT_1_PENALTY=0.073989
PRIMER_LEFT_1_SEQUENCE=AACCTTACTTGGCCTAACCTCC
PRIMER_RIGHT_1_SEQUENCE=CAATGTTTGGTGCTGTTCAGTT
PRIMER_LEFT_1=75,22
PRIMER_RIGHT_1=367,22
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.1731
PRIMER_LEFT_2_PENALTY=0.099075
PRIMER_RIGHT_2_PENALTY=0.073989
PRIMER_LEFT_2_SEQUENCE=AACCTTACTTGGCCTAACCTCC
PRIMER_RIGHT_2_SEQUENCE=AATGTTTGGTGCTGTTCAGTTG
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=366,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=292
PRIMER_PAIR_PENALTY_3=0.3100
PRIMER_LEFT_3_PENALTY=0.099075
PRIMER_RIGHT_3_PENALTY=0.210973
PRIMER_LEFT_3_SEQUENCE=AACCTTACTTGGCCTAACCTCC
PRIMER_RIGHT_3_SEQUENCE=GTTGGCACTTGAGTCGTAACAA
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=295,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=60.211
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.3814
PRIMER_LEFT_4_PENALTY=0.099075
PRIMER_RIGHT_4_PENALTY=0.282356
PRIMER_LEFT_4_SEQUENCE=AACCTTACTTGGCCTAACCTCC
PRIMER_RIGHT_4_SEQUENCE=GTGCTGTTCAGTTGGGGTTATT
PRIMER_LEFT_4=75,22
PRIMER_RIGHT_4=358,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=60.282
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_04172 Ps_contig_mira-and-tgicl-ass_franssen_32339
SEQUENCE=AAAGAAAAGAAGATTTTCTATTAACATATACATCATTCAATTTTTCATTATCATACAAGAAAGTGATTAACACATATTAAATGCACATTTGATTAAAGATTCATCAAAACCTAACAGTTAAACTAACCACCAACCCAAATTAATGTCCTAATTAGAAATTAGAAACAGTGTTTAAGCCCCATGCCATGTTTGCCACGAACACAACCAGGACTCACATAAAGATAAGGAGACCTCTTCGAATTGTTCATCTTTGATGAAACCCTTTTCCCCAATTTCCTCATTCTCTGTCTTCCCAATTTGTTCCCTATATCCCATGGAACAAGCTCATCATCCAAATAATCTTCATCATCATCATCGTCGTCTTCTTCTTCATCATCATCATCATCAATATCATGGTTAACCTTAACCTGTTCCTCACTGAACCCATCATCGAGATGAAGAAGACGGTGGTTCATTCCTTCAATTGGGTCACCGATTGGAGAAGAAACAGCCCAAGAAATGAAAGATTCTTCGTCAACACCATCGACCATTTCTTCTTCTTCTTCGGCGTCAACGACATCGACAAGTTCCCATTGAAGAAGAGAATCGTCGTTTACCTTCAAGAATTGAACTTGTTCGAGGATGAGAAACTGCATCGATGAAGAGGTTGTTGAGGATTGGTTTTCCATTTTCACGCTAAAAAACTTCTTTTTCTGTTTTCTTTCTTCTTTTTTTCTTCTTGTGCTGGTAACGTTGGTGTGTGGTGTCTACGGTTTGGTTTGTGATTGTGTTGTGTTGTTGTGTGTCT
TARGET=343,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0986
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.075027
PRIMER_LEFT_SEQUENCE=CTCTGTCTTCCCAATTTGTTCC
PRIMER_RIGHT_SEQUENCE=GAATCTTTCATTTCTTGGGCTG
PRIMER_LEFT=282,22
PRIMER_RIGHT=508,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.1325
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=CTCTGTCTTCCCAATTTGTTCC
PRIMER_RIGHT_1_SEQUENCE=CTTTCATTTCTTGGGCTGTTTC
PRIMER_LEFT_1=282,22
PRIMER_RIGHT_1=504,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.1349
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.075027
PRIMER_LEFT_2_SEQUENCE=CCCAATTTCCTCATTCTCTGTC
PRIMER_RIGHT_2_SEQUENCE=GAATCTTTCATTTCTTGGGCTG
PRIMER_LEFT_2=267,22
PRIMER_RIGHT_2=508,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.1410
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.117452
PRIMER_LEFT_3_SEQUENCE=CTCTGTCTTCCCAATTTGTTCC
PRIMER_RIGHT_3_SEQUENCE=GAACCACCGTCTTCTTCATCTC
PRIMER_LEFT_3=282,22
PRIMER_RIGHT_3=452,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=60.117
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.1534
PRIMER_LEFT_4_PENALTY=0.023556
PRIMER_RIGHT_4_PENALTY=0.129818
PRIMER_LEFT_4_SEQUENCE=CTCTGTCTTCCCAATTTGTTCC
PRIMER_RIGHT_4_SEQUENCE=AAGAAGAAATGGTCGATGGTGT
PRIMER_LEFT_4=282,22
PRIMER_RIGHT_4=537,22
PRIMER_LEFT_4_TM=59.976
PRIMER_RIGHT_4_TM=59.870
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_04173 Ps_contig_mira-and-tgicl-ass_franssen_32339
SEQUENCE=AAAGAAAAGAAGATTTTCTATTAACATATACATCATTCAATTTTTCATTATCATACAAGAAAGTGATTAACACATATTAAATGCACATTTGATTAAAGATTCATCAAAACCTAACAGTTAAACTAACCACCAACCCAAATTAATGTCCTAATTAGAAATTAGAAACAGTGTTTAAGCCCCATGCCATGTTTGCCACGAACACAACCAGGACTCACATAAAGATAAGGAGACCTCTTCGAATTGTTCATCTTTGATGAAACCCTTTTCCCCAATTTCCTCATTCTCTGTCTTCCCAATTTGTTCCCTATATCCCATGGAACAAGCTCATCATCCAAATAATCTTCATCATCATCATCGTCGTCTTCTTCTTCATCATCATCATCATCAATATCATGGTTAACCTTAACCTGTTCCTCACTGAACCCATCATCGAGATGAAGAAGACGGTGGTTCATTCCTTCAATTGGGTCACCGATTGGAGAAGAAACAGCCCAAGAAATGAAAGATTCTTCGTCAACACCATCGACCATTTCTTCTTCTTCTTCGGCGTCAACGACATCGACAAGTTCCCATTGAAGAAGAGAATCGTCGTTTACCTTCAAGAATTGAACTTGTTCGAGGATGAGAAACTGCATCGATGAAGAGGTTGTTGAGGATTGGTTTTCCATTTTCACGCTAAAAAACTTCTTTTTCTGTTTTCTTTCTTCTTTTTTTCTTCTTGTGCTGGTAACGTTGGTGTGTGGTGTCTACGGTTTGGTTTGTGATTGTGTTGTGTTGTTGTGTGTCT
TARGET=370,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0986
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.075027
PRIMER_LEFT_SEQUENCE=CTCTGTCTTCCCAATTTGTTCC
PRIMER_RIGHT_SEQUENCE=GAATCTTTCATTTCTTGGGCTG
PRIMER_LEFT=282,22
PRIMER_RIGHT=508,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.1325
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=CTCTGTCTTCCCAATTTGTTCC
PRIMER_RIGHT_1_SEQUENCE=CTTTCATTTCTTGGGCTGTTTC
PRIMER_LEFT_1=282,22
PRIMER_RIGHT_1=504,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.1349
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.075027
PRIMER_LEFT_2_SEQUENCE=CCCAATTTCCTCATTCTCTGTC
PRIMER_RIGHT_2_SEQUENCE=GAATCTTTCATTTCTTGGGCTG
PRIMER_LEFT_2=267,22
PRIMER_RIGHT_2=508,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.1410
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.117452
PRIMER_LEFT_3_SEQUENCE=CTCTGTCTTCCCAATTTGTTCC
PRIMER_RIGHT_3_SEQUENCE=GAACCACCGTCTTCTTCATCTC
PRIMER_LEFT_3=282,22
PRIMER_RIGHT_3=452,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=60.117
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.1511
PRIMER_LEFT_4_PENALTY=0.076597
PRIMER_RIGHT_4_PENALTY=0.074466
PRIMER_LEFT_4_SEQUENCE=ATCTTCATCATCATCATCGTCG
PRIMER_RIGHT_4_SEQUENCE=GACGATTCTCTTCTTCAATGGG
PRIMER_LEFT_4=338,22
PRIMER_RIGHT_4=589,22
PRIMER_LEFT_4_TM=59.923
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.1000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_04174 Ps_contig_mira-and-tgicl-ass_franssen_32339
SEQUENCE=AAAGAAAAGAAGATTTTCTATTAACATATACATCATTCAATTTTTCATTATCATACAAGAAAGTGATTAACACATATTAAATGCACATTTGATTAAAGATTCATCAAAACCTAACAGTTAAACTAACCACCAACCCAAATTAATGTCCTAATTAGAAATTAGAAACAGTGTTTAAGCCCCATGCCATGTTTGCCACGAACACAACCAGGACTCACATAAAGATAAGGAGACCTCTTCGAATTGTTCATCTTTGATGAAACCCTTTTCCCCAATTTCCTCATTCTCTGTCTTCCCAATTTGTTCCCTATATCCCATGGAACAAGCTCATCATCCAAATAATCTTCATCATCATCATCGTCGTCTTCTTCTTCATCATCATCATCATCAATATCATGGTTAACCTTAACCTGTTCCTCACTGAACCCATCATCGAGATGAAGAAGACGGTGGTTCATTCCTTCAATTGGGTCACCGATTGGAGAAGAAACAGCCCAAGAAATGAAAGATTCTTCGTCAACACCATCGACCATTTCTTCTTCTTCTTCGGCGTCAACGACATCGACAAGTTCCCATTGAAGAAGAGAATCGTCGTTTACCTTCAAGAATTGAACTTGTTCGAGGATGAGAAACTGCATCGATGAAGAGGTTGTTGAGGATTGGTTTTCCATTTTCACGCTAAAAAACTTCTTTTTCTGTTTTCTTTCTTCTTTTTTTCTTCTTGTGCTGGTAACGTTGGTGTGTGGTGTCTACGGTTTGGTTTGTGATTGTGTTGTGTTGTTGTGTGTCT
TARGET=531,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1391
PRIMER_LEFT_PENALTY=0.075027
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=CAGCCCAAGAAATGAAAGATTC
PRIMER_RIGHT_SEQUENCE=TCACAAACCAAACCGTAGACAC
PRIMER_LEFT=487,22
PRIMER_RIGHT=763,22
PRIMER_LEFT_TM=60.075
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=277
PRIMER_PAIR_PENALTY_1=0.1511
PRIMER_LEFT_1_PENALTY=0.076597
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=ATCTTCATCATCATCATCGTCG
PRIMER_RIGHT_1_SEQUENCE=GACGATTCTCTTCTTCAATGGG
PRIMER_LEFT_1=338,22
PRIMER_RIGHT_1=589,22
PRIMER_LEFT_1_TM=59.923
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.1000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.1511
PRIMER_LEFT_2_PENALTY=0.076597
PRIMER_RIGHT_2_PENALTY=0.074466
PRIMER_LEFT_2_SEQUENCE=ATCTTCATCATCATCATCGTCG
PRIMER_RIGHT_2_SEQUENCE=ACGATTCTCTTCTTCAATGGGA
PRIMER_LEFT_2=338,22
PRIMER_RIGHT_2=588,22
PRIMER_LEFT_2_TM=59.923
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.1000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.1730
PRIMER_LEFT_3_PENALTY=0.108929
PRIMER_RIGHT_3_PENALTY=0.064105
PRIMER_LEFT_3_SEQUENCE=GAAACAGCCCAAGAAATGAAAG
PRIMER_RIGHT_3_SEQUENCE=TCACAAACCAAACCGTAGACAC
PRIMER_LEFT_3=483,22
PRIMER_RIGHT_3=763,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.936
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.1769
PRIMER_LEFT_4_PENALTY=0.075027
PRIMER_RIGHT_4_PENALTY=0.101896
PRIMER_LEFT_4_SEQUENCE=CAGCCCAAGAAATGAAAGATTC
PRIMER_RIGHT_4_SEQUENCE=CAATCACAAACCAAACCGTAGA
PRIMER_LEFT_4=487,22
PRIMER_RIGHT_4=766,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_04175 Ps_contig_mira-and-tgicl-ass_franssen_32350
SEQUENCE=AGAAAATAGATAAATAAAGTNGACCGATATTTTTACCTAAAAGAACCAGCCATAGAAACACCCACCAGTAGAGAGTGAAGTGAGTGAGTCTGAAAGAAAAGAAAAGGGTTAGTTGGTTGTTGAAGCAAATTGAAGACCAAAATCATGGCTGTTTCAGTAACTCTTATAGTAGCCATCATTTCTCTGCATCTCTTTGCTTTCGTTTTCGCTATCGGCGCCGAACGACGTCGTAGTCTGGCAAAAGTGGTTCCCGATGAGTACGACGACCGATCTTTCTGCGTTTACACCACCGACGCTTCCACTGTTTACGGTCTCGCCGCCTGCTCTCTCCTGCTCCTCAGTCAAGCTATCCTTTACGCCGTTACTAGATGTCTCTGCTGCGGTAAAGGTCTTGTCTCCGGTTGTTCCACTACTTGTGCGGTTATCTTCTTTGTTCTCTCATGGCTTGGTTTTTTGGGAGCCGAGGCGTGCTTGTTAGCAGGGTCTGCGCGGAATGCATACCACACAAAATACCAAGGGTATTTTACAAAGCATGACTTGTCATGCGCCACTCTTCGCAAGGGCGTGTTTGCTGCGGGGGCTGCCTTGACCTTGTTCTCTATGTTGGCTTCCATTTTGTACTATTGGGCACACTGTAAGGCCGACACTGGCTTCTGGGAGAAGCACCAAAATGAAGGTATTGGATTGGCCACACACAATCATGGTCATGAATCTGATAAGCCCTGAAGATTTACCACTTTTAATGCCACGGGTTATTAATTTACACAATTTTGGTGGTTTTGTTTTGTTGGATAAGGTATTTTGGATGTAAAGGAAGGAACAATGTGTTTAAAAACCCATGTTGGTAGAATATATGTTATATTTATGCTGTAATAGCAGAATGCTTGAAACAATATATATATAGGTAAATAGATGATAATGGTATGGTGGAGGTTCAGTATATTCTAGTTTCCAGTTTGAAACTTGAAACAAAATCATTGCATTAGTGCATCGAATGCTTGCATTATTGTGACAAAAAATGTGCTTGATAACTTGAGTAAAATTACTATGATAGCCTTCTTTCTAGTTAG
TARGET=893,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5068
PRIMER_LEFT_PENALTY=0.127927
PRIMER_RIGHT_PENALTY=0.378922
PRIMER_LEFT_SEQUENCE=CACTTTTAATGCCACGGGTTAT
PRIMER_RIGHT_SEQUENCE=ACTGAACCTCCACCATACCATT
PRIMER_LEFT=734,22
PRIMER_RIGHT=938,22
PRIMER_LEFT_TM=60.128
PRIMER_RIGHT_TM=59.621
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.7110
PRIMER_LEFT_1_PENALTY=0.332028
PRIMER_RIGHT_1_PENALTY=0.378922
PRIMER_LEFT_1_SEQUENCE=TCATGAATCTGATAAGCCCTGA
PRIMER_RIGHT_1_SEQUENCE=ACTGAACCTCCACCATACCATT
PRIMER_LEFT_1=704,22
PRIMER_RIGHT_1=938,22
PRIMER_LEFT_1_TM=59.668
PRIMER_RIGHT_1_TM=59.621
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.9321
PRIMER_LEFT_2_PENALTY=0.553159
PRIMER_RIGHT_2_PENALTY=0.378922
PRIMER_LEFT_2_SEQUENCE=GGTCATGAATCTGATAAGCCCT
PRIMER_RIGHT_2_SEQUENCE=ACTGAACCTCCACCATACCATT
PRIMER_LEFT_2=702,22
PRIMER_RIGHT_2=938,22
PRIMER_LEFT_2_TM=59.447
PRIMER_RIGHT_2_TM=59.621
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=1.1692
PRIMER_LEFT_3_PENALTY=0.127927
PRIMER_RIGHT_3_PENALTY=1.041315
PRIMER_LEFT_3_SEQUENCE=CACTTTTAATGCCACGGGTTAT
PRIMER_RIGHT_3_SEQUENCE=TGAACCTCCACCATACCATTATC
PRIMER_LEFT_3=734,22
PRIMER_RIGHT_3=936,23
PRIMER_LEFT_3_TM=60.128
PRIMER_RIGHT_3_TM=59.959
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=1.1692
PRIMER_LEFT_4_PENALTY=0.127927
PRIMER_RIGHT_4_PENALTY=1.041315
PRIMER_LEFT_4_SEQUENCE=CACTTTTAATGCCACGGGTTAT
PRIMER_RIGHT_4_SEQUENCE=GAACCTCCACCATACCATTATCA
PRIMER_LEFT_4=734,22
PRIMER_RIGHT_4=935,23
PRIMER_LEFT_4_TM=60.128
PRIMER_RIGHT_4_TM=59.959
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_04176 Ps_contig_mira-and-tgicl-ass_franssen_32355
SEQUENCE=ACCAATAACTAGCCTTTGCTTTTCTTACATCTCTCTCACACACACACTTTTTATTATAGTTAAGGCAATGGCTATAGAATGCATAACAAGTAGTGCAAAACTCATGACTCAAAAGAGTGATAAAAATGAAAATGAAGAATCATCAAAATTAGTTTTTGATGCTTCATTTCTAAAAAACCAACTCAACCTTCCAAAACAATTCATTTGGCCAGATGATGAAAAGCCATGCATGAATGTGCCGGAACTTGATGTTCCACTCATTGATTTCAAAAACTTCCTTTCTGGTGACCCTTTTGCGGCAATGGAAGCTTCTAAAACCATTGGTGAAGCATGTGAGAAACATGGGTTTTTTCTTGTGGTTAATCATGGAATTGATACAAAGTTAATTGAACATGCTCATAGTTACATGAATGATTTCTTTGAGGTTCCCCTGTCTCAGAAACAGAGGTGTCAGAGAAAAACAGGGGAACATTGTGGTTATGCTAGTAGTTTCACTGGGAGATTCTCCTCAAATCTTCCATGGAAAGAAACTCTTTCTTTTCAATTTTCAGATGAGAAAAACTCTTCAAATATTGTTAAAGATTACCTTTCCAACACATTAGGGGAAGATTTTCAACAATTTGGGGAGGTTTACCAAGAATATTGTGAAGCAATGAGCAAACTTTCACTAGGGATAATGGAGCTACTTGGAATGAGTCTTGGAGTTGGGAAAGAATGTTTTAGAGATTTTTTTGAAGAGAATAAATCAATTATGAGACTTAATTATTACCCTCCATGTCAAAAACCTGATCTCACTTTAGGAACTGGCCCTCATTGTGATCCAACATCTTTAACTATTCTTCACCAAGATCAAGTTGGTGGCTTGCAAGTTTTTGTTGATAATGAGTGGCATTCCATTAGGCCAAATTTCAATGCTTTTGTTGTCAATATTGGTGACACCTTCATGGCTCTTTCAAATGGGAGATACAAGAGTTGTTTACATAGGGCAGTGGTGAACAACAAGACAACAAGAAAATCTCTAGCTTTCTTTTTGTGTCCAAAAGGTGATAAAGTGGTTAGCCCACCAAGTGAATTGGTGAATGATTTGACACCAAGGATCTATCCAGATTTTACATGGCCAATGCTTCTTGAGTTTACTCAAAAACATTATAGAGCTGATATGAGGACACTTGAGGCATTTACAAAATGGATTCAACAAAAACAAAACTAGCTCTATTTTCAACCACATGAATAAAAACAAATTATTAGAGCCATTGATATACAACCCTAGTGAAAAAGTGGCCAATCATGTGAAAGTACACTTGTGTAATTCATGAGGCACATTAGTCAAGAATAATGAAGAATTAGGGTTATGATTTAAATGGTTATGAAGCATCAATAAGAAAAATTTGCCACTAATTTTGGATTATTATATAGAGATGTCTATAAACTTTGACANTATTTGACTTTTTAAAA
TARGET=37,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.4492
PRIMER_LEFT_PENALTY=2.068375
PRIMER_RIGHT_PENALTY=0.380820
PRIMER_LEFT_SEQUENCE=GCCTTTGCTTTTCTTACATCTCTC
PRIMER_RIGHT_SEQUENCE=AGGGTCACCAGAAAGGAAGTTT
PRIMER_LEFT=11,24
PRIMER_RIGHT=291,22
PRIMER_LEFT_TM=59.932
PRIMER_RIGHT_TM=60.381
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=2.4492
PRIMER_LEFT_1_PENALTY=2.068375
PRIMER_RIGHT_1_PENALTY=0.380820
PRIMER_LEFT_1_SEQUENCE=GCCTTTGCTTTTCTTACATCTCTC
PRIMER_RIGHT_1_SEQUENCE=AAAGGGTCACCAGAAAGGAAGT
PRIMER_LEFT_1=11,24
PRIMER_RIGHT_1=293,22
PRIMER_LEFT_1_TM=59.932
PRIMER_RIGHT_1_TM=60.381
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=2.4492
PRIMER_LEFT_2_PENALTY=2.068375
PRIMER_RIGHT_2_PENALTY=0.380820
PRIMER_LEFT_2_SEQUENCE=GCCTTTGCTTTTCTTACATCTCTC
PRIMER_RIGHT_2_SEQUENCE=AAGGGTCACCAGAAAGGAAGTT
PRIMER_LEFT_2=11,24
PRIMER_RIGHT_2=292,22
PRIMER_LEFT_2_TM=59.932
PRIMER_RIGHT_2_TM=60.381
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=2.6574
PRIMER_LEFT_3_PENALTY=2.068375
PRIMER_RIGHT_3_PENALTY=0.589035
PRIMER_LEFT_3_SEQUENCE=GCCTTTGCTTTTCTTACATCTCTC
PRIMER_RIGHT_3_SEQUENCE=GTTTTGGAAGGTTGAGTTGGTT
PRIMER_LEFT_3=11,24
PRIMER_RIGHT_3=196,22
PRIMER_LEFT_3_TM=59.932
PRIMER_RIGHT_3_TM=59.411
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=2.7915
PRIMER_LEFT_4_PENALTY=2.068375
PRIMER_RIGHT_4_PENALTY=0.723090
PRIMER_LEFT_4_SEQUENCE=GCCTTTGCTTTTCTTACATCTCTC
PRIMER_RIGHT_4_SEQUENCE=GGGTCACCAGAAAGGAAGTTTT
PRIMER_LEFT_4=11,24
PRIMER_RIGHT_4=290,22
PRIMER_LEFT_4_TM=59.932
PRIMER_RIGHT_4_TM=60.723
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_04177 Ps_contig_mira-and-tgicl-ass_franssen_32357
SEQUENCE=TGAGAAACAAAATACACAGAAACCACATTTCCATTCCTCTTCCTCACCAACATAACTTGCTTCTCTTTGTCCAGTCCAAAACTCAACACAACTCCTAATTTCATTCTTTATCCAACCATCATCATACATAAATTCTTCTTCTTCCAATACTGACTGATCCCTCTGAGATTCATATCTCAACACAAGCTTATCGCTAGCAGGGGACAACTATTTTACACGTTATTTTGGTAACACACCACCAAATCAGAGAGTCTCGATGCTGAGATTCCGGAATCAACGCACGCTGTCTGTAGATCTTTGCATAAGAAACGTCGAGGATTTAACTCGAAATAATCGAATAGTTGTTTAGAAGGGAAATCATGATCTGCATGAGCAACTAAATTGTCCCACAAATA
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1319
PRIMER_LEFT_PENALTY=0.050763
PRIMER_RIGHT_PENALTY=0.081185
PRIMER_LEFT_SEQUENCE=GTCCAGTCCAAAACTCAACACA
PRIMER_RIGHT_SEQUENCE=CAAAGATCTACAGACAGCGTGC
PRIMER_LEFT=68,22
PRIMER_RIGHT=299,22
PRIMER_LEFT_TM=60.051
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.1319
PRIMER_LEFT_1_PENALTY=0.050763
PRIMER_RIGHT_1_PENALTY=0.081185
PRIMER_LEFT_1_SEQUENCE=GTCCAGTCCAAAACTCAACACA
PRIMER_RIGHT_1_SEQUENCE=TGCAAAGATCTACAGACAGCGT
PRIMER_LEFT_1=68,22
PRIMER_RIGHT_1=301,22
PRIMER_LEFT_1_TM=60.051
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.1319
PRIMER_LEFT_2_PENALTY=0.050763
PRIMER_RIGHT_2_PENALTY=0.081185
PRIMER_LEFT_2_SEQUENCE=GTCCAGTCCAAAACTCAACACA
PRIMER_RIGHT_2_SEQUENCE=GCAAAGATCTACAGACAGCGTG
PRIMER_LEFT_2=68,22
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=60.051
PRIMER_RIGHT_2_TM=60.081
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.1319
PRIMER_LEFT_3_PENALTY=0.050763
PRIMER_RIGHT_3_PENALTY=0.081185
PRIMER_LEFT_3_SEQUENCE=TGTCCAGTCCAAAACTCAACAC
PRIMER_RIGHT_3_SEQUENCE=CAAAGATCTACAGACAGCGTGC
PRIMER_LEFT_3=67,22
PRIMER_RIGHT_3=299,22
PRIMER_LEFT_3_TM=60.051
PRIMER_RIGHT_3_TM=60.081
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.1319
PRIMER_LEFT_4_PENALTY=0.050763
PRIMER_RIGHT_4_PENALTY=0.081185
PRIMER_LEFT_4_SEQUENCE=TGTCCAGTCCAAAACTCAACAC
PRIMER_RIGHT_4_SEQUENCE=TGCAAAGATCTACAGACAGCGT
PRIMER_LEFT_4=67,22
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=60.051
PRIMER_RIGHT_4_TM=60.081
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_04178 Ps_contig_mira-and-tgicl-ass_franssen_32358
SEQUENCE=TATATATTCCAAAACTTTCTTTTCTATTTTNTCGGGGATCCTTTTCTCTTGTTTTCTCTCTTTCTTCTTCAAAAATGAAGTTCCAAGAGAAAGACCATCTCTATGGAAACAACAACAACAAAAAACAAATGATCCCAAAGCTAATCTTAACAGCAATCTTTGTCACAATTCTTGTTCTTGTAACCCCTTTATCATATCCTTTGTTTGGTTCATCTTTACTCTTGATGATGAATCAAACAAAGTCTAAGCAATTACCATCATCTTCTACTCCAAATATTGAACCAGAAACTTTACCTTCAACATCATTGAAAACTTGTGACATTTTCAGTGGTGAATGGATTCCGAATCCGAAAGCACCATATTACACAAACAAAACATGTTGGGCAATTCATGAACATCAAAATTGTATGAAATATGGAAGACCAGATTCTGATTTCATGAAGTGGAGATGGAAACCAAATGAGTGTGAGTTGCCAATTTTCAATCCTTTTCAGTTTCTTGAAATTGTTAGAGGAAAATCTATGGCTTTTGTTGGAGATTCTGTTGGGAGAAATCACATGCAGTCTTTGATTTGTCTTCTATCAAGAGTGGAATGGCCCATTGATGTATCATCCACAAAAGATGAATATTTCATGAGATGGAAATATCCAAGTTACAATTTCACCATGGCTGTTTATTGGACACCATTTCTTGTCAAAACAAAACAAGAAAATTCAGATGGGCCAACACACACTGGTCTCTACAACATTTATCTTGATGAGTTTGATGAAAAATGGACATCAGAAATTGAAGATTTTGACTATGTCATAATCAATGGAGGACATTGGTTCTACAAGCCAATGGTTTTCTATGAGAAAGGAAAAATTGTTGGATGCCATTATTGTCTCTTAGAAAATGTGACTGATTTAACAATGTATTATGGCTATAGAAAGGCTTTTAGGACAGCATTTAGAGCCTTAAATAGTTTAGAAAATTTTAAGGGTGTGACATTTCTTAGGACATTTGCACCATCTCATTTTGAGAATGGTATATGGAATCAAGGTGGGAACTGTGTGAGGACTAAGCCATTTAAGAGTAATGAGGCTAGGCTAGAAGGGACTAATATGGAGTTATATATGATTCAGTTGGAGGAGTATAAAATTTCTCAAAAAAGGGCAAAAAGAAATGGTTTGAAATTCAGATTGCTCGATACTACGCAATCGATGTTATTGCGACCGGACGGACATCCAAGTAAGTATGGACATTTGCCGGGAGAGAATGTGACTTTGTATAACGATTGTGTCCATTGGTGTTTGCCTGGACCGATTGATACATGGAATGATTTCTTACTTGAAATGTTGAAGATGGAGATTGTTAAGTCTAAGAAAGAAAGGCGTCGCATTGATTCATAATTAGATAGATTTTAAATATTATTTATGTATGGCTAATATTTCTTATAAACTATAATTGCCATATTTATTGAATTTTGTAGAGATAATTTTTGTTATATTTATAATAAATTATATT
TARGET=108,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2082
PRIMER_LEFT_PENALTY=1.044540
PRIMER_RIGHT_PENALTY=0.163658
PRIMER_LEFT_SEQUENCE=TGAAGTTCCAAGAGAAAGACCA
PRIMER_RIGHT_SEQUENCE=TCCATTCACCACTGAAAATGTC
PRIMER_LEFT=75,22
PRIMER_RIGHT=338,22
PRIMER_LEFT_TM=58.955
PRIMER_RIGHT_TM=59.836
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=1.5846
PRIMER_LEFT_1_PENALTY=0.173582
PRIMER_RIGHT_1_PENALTY=1.411021
PRIMER_LEFT_1_SEQUENCE=GGGGATCCTTTTCTCTTGTTTT
PRIMER_RIGHT_1_SEQUENCE=TGCTGTTAAGATTAGCTTTGGG
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=154,22
PRIMER_LEFT_1_TM=59.826
PRIMER_RIGHT_1_TM=58.589
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=2.1244
PRIMER_LEFT_2_PENALTY=1.044540
PRIMER_RIGHT_2_PENALTY=1.079866
PRIMER_LEFT_2_SEQUENCE=TGAAGTTCCAAGAGAAAGACCA
PRIMER_RIGHT_2_SEQUENCE=GGAATCCATTCACCACTGAAAA
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=342,22
PRIMER_LEFT_2_TM=58.955
PRIMER_RIGHT_2_TM=61.080
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=2.2627
PRIMER_LEFT_3_PENALTY=1.044540
PRIMER_RIGHT_3_PENALTY=1.218111
PRIMER_LEFT_3_SEQUENCE=TGAAGTTCCAAGAGAAAGACCA
PRIMER_RIGHT_3_SEQUENCE=GGAATCCATTCACCACTGAAA
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=342,21
PRIMER_LEFT_3_TM=58.955
PRIMER_RIGHT_3_TM=59.782
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=2.3699
PRIMER_LEFT_4_PENALTY=1.044540
PRIMER_RIGHT_4_PENALTY=1.325343
PRIMER_LEFT_4_SEQUENCE=TGAAGTTCCAAGAGAAAGACCA
PRIMER_RIGHT_4_SEQUENCE=TCACCACTGAAAATGTCACAAG
PRIMER_LEFT_4=75,22
PRIMER_RIGHT_4=333,22
PRIMER_LEFT_4_TM=58.955
PRIMER_RIGHT_4_TM=58.675
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=259
=
PRIMER_SEQUENCE_ID=SSR_04179 Ps_contig_mira-and-tgicl-ass_franssen_32359
SEQUENCE=TATCTTTAATATTATTCCACCGTTATACTAAACTTTCATTTCACATCCACTAACAAAACAAATTTGATTTCACATCCACTAACAAGAAACAGCAAATTAGTACATGATCGATCCATAAAAAATGACATCAAAATAACAAATACGGTCTCTGATCTAGTATCAAAATTAACATGCTTCTGTATATGATTTTTCGCCTAATATTGCAACCTTTTTTGTTGGTTTGAGTCTAATTGTGAGCTGAGGTGTGGAAAAATGGCCATGCAGGTACTCATTGAAAAAGTTAAACTGCCTTTGGCTTTGACTTAATTCGCTTCACCCTTGAGTAGAGGAAAACGCCAGCAAGACCAACGGCGGTACCTAAAGCATTCACAGGAGAGACGGGTGTTTTGAAGATGATCACAGAGCTCACAATAACCACGACACGCTTCACACAATTGCCGACGGAATGAGTAACAGGTGATACCCTCTGCAATATCATGTAAGAAACTTGTTGATATGCATGGAAACAGAGTGCAGCAAGAAGAGATCTGGTGTACACTTGTCTAACATTTAAACCAGCAGATTGCAGGTAAGCAGGGGTGAATTTGACACCCTCCATGAAGATAGCAGCAGGTGCTAACAAGAAGAAGGACATTATTGTTATTATAGAGAAGAGAGTTATGTTGTCCAAGGATTCCTCTTGTTTAACCATGACCTTTTTGCTCAGAACATTACGTGATTGGTTTGTCACATTGGAAGCCATTGCACTCCAAAATCCAGCCCAATTGAAAGAGGCCTCAGTAACAGACGCCAGAGCAACTCCACCAACAATAGGAACAAGGGAACCAATCACCCAGGGTGTAGGCCTCTCTCCAAGAAACATGGCAGAAAGGATAACAGAAAAGAAAGGCTCCATAGCTTTAATAGTGTGAGTAAAAGACACAGCCACCTTTCCAAGACTCATGTTGGTGAAAAGATTCCCCAAAGTATGCACAATAGCTAGTGGAAATATAGCTGCAAGCATAGCGCCATTGATTTTAGGCCTCTTGTAGAGATTAAGAGCCCACATAACTGATACAAGAACAGTCCCAACAGCAAACTGAACTACAGTAACAGTTACAGGGAAATGGCACGCCTTCAAAACCTGTTTATTGTAGATATTGAAGTAGATGTTGAAGAGGTACCACAATCCGAACAAGGATCCAAGTTGAAGAGTTTTGAGCAAAGAACTGGAGTCAGCAGATTCAGCGGCGCTTTCGGAAGTGGCTTTAGGAGGAGAAAGATCGGAAGTGGAAAGGAGAGGAAGAGGTCTGAATTTGAATGAAGAGGAAGGAGAAAGAGACCAGGATTTACGAGTGAAGGAAGAGGAAGAAGAAGAAGAGGCGCCATTGGGATTGGAATTGGGATTGGGATTGACGCCGCCGTTTAATTTGGCGGAGAGACGGAGGTTAGTGGAGGAGCGAGTGAAACGGTGGTTGAGATTACGGAGAGGGAGAGAAGGGGAGAGAGTGAAAGCGGCGGACTGCATTTTGAAGATCTGAAACGGAAGAGGGCGGACGTTTCTATAAGTATGGGAATGAATATCCCTTGAGGAGTCCGCCACCAGCTACAATGTTTGTTTGGTGAGGGAGTAGGTGAAATACCTTAAGACATACACTGTTTGATTGCACGACCAAACTACCYCNTTTTCAAGTGGAATATACTT
TARGET=1347,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04180 Ps_contig_mira-and-tgicl-ass_franssen_32362
SEQUENCE=AGGGAGGAGTAATCATGTGGCTATCGATTTGATTGTTCAGCATATCCGCNCAAAGCTTGGTCAGCATGACCTTTGTAAATATATCCGAATTTATATGTCATTCAGTCGACATTTCAGATACGTGGTATGCATACCTTGATACGTGATGCTCAGATAACGAAGCATGATTTTGTATTTTATTCTGATCGTTTGATTCGTTTGGTCGTAGAACACGGTTTAGGACATCTTCCATTTACAGAAAAACAAGTAATCACTCCCACAGGTTCTGTTTACACTGGTGTGGATTTTTGCAAAAGGTTGTGTGGTGTCTCTGTCATCAGAAGTGGGGAGAGTATGGAGAATGCCTTAAGAGCATGCTGTAAAGGTATTAAGATTGGAAAAATTCTGATCCACAGAGAAGGCGACAATGGTGAACAGCTAATATATGAAAAATTGCCTAACGACATCTCAGAAAGGCATGTGTTACTATTGGACCCTATTCTCGGCACAGGTAATTCGGCTGTTCATGCAATTTCTTTACTTATAAGAAAGGGTGTACCAGAATCCAACATTATATTTCTTAATCTCATCTCTGCTCCTAAAGGGGTTCACGTGGTCTGCAAAAGTTTTCCAAGAATTAAAATAGTGACATCTGAGATAGAGATTGGTTTGAATGAAGATTTCCGTGTCATACCGGGAATGGGCGAGTTTGGAGATCGGTACTTTGGAACAGATGATGATGATGAACAAGTGGTGGTTGCTTCACAGAAGTGAAACCAAATTCAACAAAATCTTTTGGTTAATCATAATTACAAGAAACTTTCTATGCTGCAATTTGTTGCTCAATTTCTACTATGCTAGAGGGTCTGAATTTTAGTTCAACATCAATTTAGACTCTACTAGGCCAGGAATAACTATGATAATTTGATGTTATCTTTTACTACTATATTCTATAACAAAAATAATTGGTATGCGATTGAGACTTCATTAACCTTGTAATGATTTACAAGTTGTGACCTTATTTTGTATATGGATGGTAGAGCATTTCAGAGNCAANTATTTAATGAAATTTT
TARGET=712,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3512
PRIMER_LEFT_PENALTY=0.016950
PRIMER_RIGHT_PENALTY=0.334292
PRIMER_LEFT_SEQUENCE=GGGTGTACCAGAATCCAACATT
PRIMER_RIGHT_SEQUENCE=TTCACTTCTGTGAAGCAACCAC
PRIMER_LEFT=530,22
PRIMER_RIGHT=753,22
PRIMER_LEFT_TM=59.983
PRIMER_RIGHT_TM=60.334
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.3578
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.334292
PRIMER_LEFT_1_SEQUENCE=TCTCATCTCTGCTCCTAAAGGG
PRIMER_RIGHT_1_SEQUENCE=TTCACTTCTGTGAAGCAACCAC
PRIMER_LEFT_1=563,22
PRIMER_RIGHT_1=753,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.334
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.4052
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.402858
PRIMER_LEFT_2_SEQUENCE=GGAGATCGGTACTTTGGAACAG
PRIMER_RIGHT_2_SEQUENCE=ATGAAGTCTCAATCGCATACCA
PRIMER_LEFT_2=690,22
PRIMER_RIGHT_2=966,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=59.597
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.4071
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.404834
PRIMER_LEFT_3_SEQUENCE=GGAGATCGGTACTTTGGAACAG
PRIMER_RIGHT_3_SEQUENCE=GAGCAACAAATTGCAGCATAGA
PRIMER_LEFT_3=690,22
PRIMER_RIGHT_3=822,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=60.405
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=0.4218
PRIMER_LEFT_4_PENALTY=0.016950
PRIMER_RIGHT_4_PENALTY=0.404834
PRIMER_LEFT_4_SEQUENCE=GGGTGTACCAGAATCCAACATT
PRIMER_RIGHT_4_SEQUENCE=GAGCAACAAATTGCAGCATAGA
PRIMER_LEFT_4=530,22
PRIMER_RIGHT_4=822,22
PRIMER_LEFT_4_TM=59.983
PRIMER_RIGHT_4_TM=60.405
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_04181 Ps_contig_mira-and-tgicl-ass_franssen_32363
SEQUENCE=TTCTTGTCCATTTTTTTTGTTTCTTATACAGAATCAGTTACAAATGTATGCATCTAAATGTATACAAATAACATACAGTATTGATTTGTACAACTCATCAACTTGAACAATAATTCGTGTATTTATTATTAACTCAAAATGAGTTTCAGGTCTACTATTTGCAATGTTACAAGAGTTCAAAACCCACTATGACAGTGGCAACGTTTCCAGGCATCAATATCATTTAGCTCTGCTCATTACTAAGATTATTATGAACTCCTCCTCCTCCTTCTCTAGGTAAAGCCTGATCAAAAGGATGCGGATATTTGTTATGAACTTTCTACGGTATCGAAAGGGACCATCTGTTATCCACATAACGCTTCCTATGTATTGTGTAATGTAAGAAAACTACATAGATATACAATGACTTGTCTGGCTTCTGAAAACATATAATCCAAACATTTGGTTTCAACTTTGAAGCAATCCGTTGAGACACTTGTCGAAGAAAGTTTCCAAGTAGCCTTTTCATCACAAACCATTTCAAGAGCCAGTGCCAAAAGTGGTGAATCTCTGTCATTAGACAAGGTAGTAATCAGACAGGAGAGCAGCTCCAATGACAACTGACAAGCACAATAAACTTACAACATCTGCCATCCCAAAAGATGATTCAGACAAGAAATAAATTTTGTGACTTTCGACGTCCCCTTTAAAACCTCTAGCTATCATAGCCTGAGAAATTTGCTCTGCATGGCTGAAAATATTTTTGAAGATGCGACGGAAGTAGTTAAAGAAAATATCAATTGTTTCCATAACAGTTAGTTGTTTCCAATTTACCCTACGAGATACAATTCCCATAGCAATGTTCCGGACCTCGTCAAAAACTAGACTGATAAATCTCAATGACAGTAAAAGGGTAAGCACAATTTCTGATACAGATACACCTATGTATCTCAGAGGAAGCATAAACCATCGCAATGCAGATGCTAGTTGTTCAGGTGTTG
TARGET=256,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2415
PRIMER_LEFT_PENALTY=0.211181
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=AACGTTTCCAGGCATCAATATC
PRIMER_RIGHT_SEQUENCE=TTTTCAGAAGCCAGACAAGTCA
PRIMER_LEFT=199,22
PRIMER_RIGHT=424,22
PRIMER_LEFT_TM=60.211
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=226
PRIMER_PAIR_PENALTY_1=0.3981
PRIMER_LEFT_1_PENALTY=0.211181
PRIMER_RIGHT_1_PENALTY=0.186949
PRIMER_LEFT_1_SEQUENCE=AACGTTTCCAGGCATCAATATC
PRIMER_RIGHT_1_SEQUENCE=TGGATAACAGATGGTCCCTTTC
PRIMER_LEFT_1=199,22
PRIMER_RIGHT_1=350,22
PRIMER_LEFT_1_TM=60.211
PRIMER_RIGHT_1_TM=60.187
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.6299
PRIMER_LEFT_2_PENALTY=0.211181
PRIMER_RIGHT_2_PENALTY=0.418705
PRIMER_LEFT_2_SEQUENCE=AACGTTTCCAGGCATCAATATC
PRIMER_RIGHT_2_SEQUENCE=ATGTGGATAACAGATGGTCCCT
PRIMER_LEFT_2=199,22
PRIMER_RIGHT_2=353,22
PRIMER_LEFT_2_TM=60.211
PRIMER_RIGHT_2_TM=59.581
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.6427
PRIMER_LEFT_3_PENALTY=0.211181
PRIMER_RIGHT_3_PENALTY=0.431486
PRIMER_LEFT_3_SEQUENCE=AACGTTTCCAGGCATCAATATC
PRIMER_RIGHT_3_SEQUENCE=TCAGAAGCCAGACAAGTCATTG
PRIMER_LEFT_3=199,22
PRIMER_RIGHT_3=421,22
PRIMER_LEFT_3_TM=60.211
PRIMER_RIGHT_3_TM=60.431
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.5000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.6766
PRIMER_LEFT_4_PENALTY=0.211181
PRIMER_RIGHT_4_PENALTY=0.465411
PRIMER_LEFT_4_SEQUENCE=AACGTTTCCAGGCATCAATATC
PRIMER_RIGHT_4_SEQUENCE=TTCTTCGACAAGTGTCTCAACG
PRIMER_LEFT_4=199,22
PRIMER_RIGHT_4=485,22
PRIMER_LEFT_4_TM=60.211
PRIMER_RIGHT_4_TM=60.465
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.5000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_04182 Ps_contig_mira-and-tgicl-ass_franssen_32366
SEQUENCE=TCGGGGAATGAAGCCACAACAGCTTCAACAATAAGAGACAGGTAGTTGAAGAAACGAACCCTAACATAAGAGAGAGAAAGTTAACAGCTTTGCTTTGATTTCAAGCCCCTTTTACCATTTGCATTTAGGGATCTCGAATCTTCAATCTACAAAGTTATTGATCCCGCTTCTATGGCAAATTCAAAGAGCTCATCGGGTGTCAGAAATTTTGTGTATCCTGGAAAGCAGGCTCATGCTTTGCTTCCTCCCAAAAGCCCATTTCCTAGTATTTCTCAAGCATATGCTGATTATGTCTCAAATCCTACCATTGGTTCAAGACCTGTTAATAAGCCAAGAGAAGGAAACGCGCACCACCAACGTACTTCATCTGAGAGCCATCTTATTGAGGAGCAGCCATCATGGCTCGATGATCTCCTAAATGAGCCTGATACACCTGTCAGGAAGGGTGGTCATAGGCGTTCATCAAGTGATTCTTTTGCATATATCGACACTCTTACTGCCCCGAACATCAGTTATGCGGATCATGATGAGTATAAATATAAGAATTTCTTATCTATTCCGGCTTGGTCCCCTCCAGATTTTGATCGTAACAAAGATGCTCGTCATGTTCCCATGTATTCAGAAATGAATGCAGCAAAACAGAAAAATAGATCATGGGACTCTTTTTCAAATATGTGTGGCGCTCCATCTGGCAAAGACAGCGTAGCTTTTCAGAGATCAGGATCACCAACCCCATCTACACTGTATGAAGCAGATCGGATCCTACCAACTGCAAATGAAAATTATGATTCAGTAGAATCTGGCTTACAAGACATTAGATCATCTTTTGAAAGAAAGGATGGTTTACATGCAAAGTCATCTGCCTCTGAAACAGATACAAAACGTGCTAAACAGCAATTTGCTCAACGATCACGGGTTCGTAAACTTCAGTACATAGCCGAGCTGGAAAGAAATGTACAGTCTTTACAGGCAGAAGGGTCTGAAGTATCAGCTGAGCTTGAGTTTCTTAACCAGCAGAATCTTATCCTGAGTATGGAGAATAAAGCACTCAAACAGCGTTTAGAAAGTTTAGCACAGGAGCAGCTTATCAAATACTTGGAGCAGGAAGTACTGGAAAGAGAGATTGGAAGATTACGCGGCATGTATCAACAGAATCTGCAGCAGTCACATCAGCCTCAACAACAACCACCTGGTAGTCATAGACGCACAAACAGCAGAGACCTTGACTCTCAATTTGCTAACCTTTCTCTGAAGCACAAGGACACCAATTCGGGGCACGACCCTTCAAATGGAGCACTCAGAATTTAGATGTTGCCACCCCCTGTGATAAAATTTGTTTCCTTCCAACATGCGGGATTGTGCAGAGATTTCCAGGATGTGCGCCCTCTCTCTCTGCCCGCGTTGCTGATATTTCAGCGTTCGTTTTAAGTTGTTATTATGTTCTCTATTTACTTAAACTATTGCCATTTATTTAATGTTGCACCTGGCGGTCATTGCTGAAATCTTAATTCTAATTTCTGTGACTTGACCTGCCGGAGGTGGTAACAATGGTTTTGAAGCTTTCACTCCTACTGAATCTCCTTGAGTCCAGTGATTATGTAACTTAATACCTCCTCTTGTGAATCTCCGTTGTGTTGGAAGTTGGTCCAGTGCAAAGAGCAGATAGATATCTTGGGGCCATTTGATGTGCATTATTATTTGTATGGATATCCTTTAGCTAATATTTGTAATGTGATTTTAATGGAGTTCATCTTTTCTGAACACATATTATCTGTTTGCAGTGAGAAGATTAGATTGACTTTGGACGTTGTTGTTGTCGGTTATTATTTCCTAAAATATAAGTTCAGCTTTCATGTGTAGACCAAGGCTAGGAAGCTTTAGAGTGTTATNRACNTCAATTTTAGAGTCTTATGCTATCTAATTTTATTCTTTTATGTTTACT
TARGET=1385,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04183 Ps_contig_mira-and-tgicl-ass_franssen_32368
SEQUENCE=GGGGAACAAACTTTTTTCTAACAAACAAACATTACTTCAGACAGCGTCAGCATCCTTAATCCAAACTTGGAACTTGTCTTGACTTTACTCCTTCTCACCTTCTCACCTTCTCTCACATGGAGAACTCGATCTCCGGCGACGACTACATCTACCAATCCGGCTTCGGCAACCACTTCTCCTCGGAGGCCATCGCCGGAGCTCTCCCGGTTCATCAAAACAGCCCCCTCATATGCCCGTTCGGCCTCTACGCCGAACAAATATCCGGAACCTCCTTTACCACCCCTCGTTCCCACAACCTCTTCAGTTGGCTTTATCGGATAAAACCATCGGTGACTCACGAACCGTTCAAGCTTCGGGTTCCGTCTAATCGGAAAATTTTGAGTGAGTTCAACGACTCAAACAGTTCTACAAATCCAACTCAGCTTCGATGGAAACCAGCAGATATACCTGATTTACCCACGGATTTTATTGATGGGTTGTCCACCGTTTGTGGTTCTGGCAGCTCATTCATGCGCCATGGATATGCTATTCACATGTACACTGCTAACAAATCAATGGACAATTGTGCCTTTTGCAATGCTGATGGCGATTTCTTGATAGTTCCCCAACAAGGAAGACTCTTCATCACTACTGAATGTGGAAAGTTGAAAGTTTCCCCAGGTGATATTGCTATAATACCTCAAGGTTTTCGTTTCAACGTGGATCTTCCTGATGGTCCCTCCCGTGGTTATGTAGCTGAAATTTTCGGTACTCATTTTCAACTTCCTGATCTTGGATCAATAGGCGCTAATGGTCTTGCTTCCCCAAGGGACTTCCTTGTTCCTACAGCATGGTTTGAAGATAAATCATATCCTGGATACACTATAGTGCAAAAGTTTGGCGGTGAACTATTTACTGCAGTACAAGATTTCTCTCCCTTCAATGTTGTTGCTTGGCATGGTAATTATGTTCCATATAAGTATGACCTAAGCAAATTTTGCCCTTATAATACAGTGTTGTACGATCACGGCGATCCATCAATTAATACTGTGTTGACAGCACCAACTGATAAACCCGGCGTGGCATTGCTTGATTTTGTCATTTTTCCACCAAGGTGGCTTGTTGCTGAGCATACCTTCCGTCCACCATATTATCATCGCAACTGCATGAGTGAATTTATGGGTCTCATTCATGGTAACTATGAGGCAAAAGTTGATGGATTTCTTCCGGGTGGTGCAAGTCTTCATAACTGTATGACTCCGCATGGTCCTGATACCAAGTCATATGAGGCCACCATTGCCCGTGGGAATGATGTTGGTCCTCACAAGATCACCGACACAATGGCTTTTATGTTTGAATCAAGTTTGATTCCCCGTATCAGCCGATCGGCTCTCGAATCTCCGTTCCTGGATCATGATTATTACCAATGTTGGATCGGTTTGAAATCTCATTTCGCAAGTTCCTGAGACTTCTCTTGGAAGCCTAACTTTGAACAATGGAGAGTGAAAGTTATATGTACATACATAAAAGTATGGAGAGTGAAAGTTTTATGTACATACATAAAAATAAAAAGAATCAGTACGAGATGTAATCTAGATGTTAAAAGTTAGTAAATAAAATAATTTT
TARGET=20,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04184 Ps_contig_mira-and-tgicl-ass_franssen_32369
SEQUENCE=TGTTATTTNGCGTGTGAGGAAAGTGTGATCAAAGTGAAGATGCCTTGTCTCAACCTTTCAACAAACGTTAGCTTTGATGGTGTTGATGTGTCTTCCATACTTTCTCAACTCAACTCAACTCTTGCCAACATCTTGAATTACCCTCAATCTTGGGTGACAGTTTCATTAGAAGGATCAATACCCATGTGTTTTGGAAACACAGAGGAGCCAGCTGCTTATGGTGAATTTGTGGCCATTGGTATTCTCAATCCAGAGTTGAACAAGAAACTCTGTGCTGAAATTGCCTTAGTTCTTCAGACCAAGTTGTCAGTTCCCAAGTCAAGATTCTTCTTAAAATTCAATGATATTGAGGGTTATAATTGTGGCTTGCATGGCGGTCTCATGGTGGTAGAATCCAAATGATTGCATAATGAAATTTGTTTGGAGAGTCTGAAGCTAATATGGTACTATTGTACATATTTGGTGTACAATAAGGCAATGAAGTTTGAAACTTAAATGTCTTTTATTGCCATCGGAAGTGGATGAAATAGTTCAACTGATATGCATTAATTAAATTAAAATAAGTGTAGCTGCAAATGTGTAGCTGCAATTCTACTATTAAGTGACCTCAGTTCCTTCTTGAAATCTACAGAAAATCGATTTTTTAAACGGTT
TARGET=104,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2440
PRIMER_LEFT_PENALTY=0.182028
PRIMER_RIGHT_PENALTY=0.061961
PRIMER_LEFT_SEQUENCE=TGTCTCAACCTTTCAACAAACG
PRIMER_RIGHT_SEQUENCE=ACTTGGGAACTGACAACTTGGT
PRIMER_LEFT=45,22
PRIMER_RIGHT=318,22
PRIMER_LEFT_TM=60.182
PRIMER_RIGHT_TM=59.938
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.2638
PRIMER_LEFT_1_PENALTY=0.182028
PRIMER_RIGHT_1_PENALTY=0.081801
PRIMER_LEFT_1_SEQUENCE=TGTCTCAACCTTTCAACAAACG
PRIMER_RIGHT_1_SEQUENCE=GAGAATACCAATGGCCACAAAT
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=245,22
PRIMER_LEFT_1_TM=60.182
PRIMER_RIGHT_1_TM=60.082
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.3050
PRIMER_LEFT_2_PENALTY=0.243031
PRIMER_RIGHT_2_PENALTY=0.061961
PRIMER_LEFT_2_SEQUENCE=AGATGCCTTGTCTCAACCTTTC
PRIMER_RIGHT_2_SEQUENCE=ACTTGGGAACTGACAACTTGGT
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=318,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=59.938
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.3050
PRIMER_LEFT_3_PENALTY=0.243031
PRIMER_RIGHT_3_PENALTY=0.061961
PRIMER_LEFT_3_SEQUENCE=GAAGATGCCTTGTCTCAACCTT
PRIMER_RIGHT_3_SEQUENCE=ACTTGGGAACTGACAACTTGGT
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=318,22
PRIMER_LEFT_3_TM=59.757
PRIMER_RIGHT_3_TM=59.938
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.3109
PRIMER_LEFT_4_PENALTY=0.248900
PRIMER_RIGHT_4_PENALTY=0.061961
PRIMER_LEFT_4_SEQUENCE=TGATCAAAGTGAAGATGCCTTG
PRIMER_RIGHT_4_SEQUENCE=ACTTGGGAACTGACAACTTGGT
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=318,22
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=59.938
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_04185 Ps_contig_mira-and-tgicl-ass_franssen_32371
SEQUENCE=TGATAAGGAAGATTTTGATGGGAATAGTGGTTTATGTGGTGGGCCACTTGGTTCAAAGTGTGGTAGTATGAGTAAGAAGAATCTTGCTATTATTATTGCTGCTGGTGTTTTCGGTGCGGCGGGTTCTTTGTTGTTGGCTTTTGGGTTGTGGTGGTGGTATCATATTAGGTTGAGGAGGGAGAGGAGTAAGGAAGGGTATGTTGTTGGTGGGGTTGACGATTGGGCGTTGAGGTTGAGAGGTCATAAGCTTGCGCAAGTTAATCTTTTTCAGAAACCGATTGTTAAGGTTAAGCTTGGTGATTTGATGGCTGCGACGAATAATTTTAGTTCGGAGAATGTTTTGATTACGACGAGGACGGGTGCGACGTATAGGGCGGATTTACCGGATGGTTCGACGCTTGCGGTGAAGCGGTTGAGTACTTGTAAGATTGGTGAGAAGCAGTTTAGGATGGAGATGAATCGGTTGGGACAGCTTAGGCATCCGAATTTGGCGCCTTTGTTAGGGTATTGTGTTGTGGAAGAGGAGAAGCTTTTGGTTTATAAGCATATGTCAAACGGGACGCTTTATTCGTTGTTGCATAAGAATAGCGGCACGTTGGATTGGTTGATGAGGTTTAGGATAGGGTTGGGTGCGGCGAGGGGACTTGCGTGGTTGCATCATGGTTGTCATCCTCCGATTATACAGCAGAATATTTGTTCTAATGTGATTCTTGTTGACGAAGAATTCGACGCTCGGATAATGGACTTTGGACTCGCGAGGCTTATGACGTCTGATTCCAACGGTAGTTTTGTGAACGGAGATTTGGGGGAGCTAGGTTATATCGCTCCTGAGTATTCGAGTACGATGGTTGCCTCGTTGAAAGGAGACGTGTATGGATTCGGAGTTTTGCTTTTGGAGTTGGTCACAGGGTGCAAGCCTCTCGAGGTGAACCGCGGCGATGAAGAATTTAAGGGTAACTTAGTGGATTGGGTGAATGTGCATTCTAGTTCAGGTAGACTCAAGGATTGCATCGATAAAGCGATATGTGGTAAAGGTAACGACGAAGAGATTCTGCAGTTTCTGAAAATCGCGTCCAATTGCATCGTAGCTCGCCCGAAAGATAGGTGGTCTATGTATCAAGTTTATCATTCATTGAAAACCATTTCCAAAGACAATAGTTTCTCCGAACAAGACGACGAGTTTCCATTGATCTTCGGTAAGCCAGAAAATGAGCCGTCTTAGTTCGCGCACGAGCTTGGCAAAAGAAGAAGAAGACATAAACCGAGGTTTTACCGAGTTCCTAGTTATGTTCATGGTTGACACTAATCAGAGGTATAATTGTTCTTGTATGATATAAGAATGCTTGTGGTATAGATTAGGTTATTGTGATTTATGAAATCTAATATGTTTCAATGCAACCTTAAATGTTGTCTAAGATATTTGGAGTATGTTTAAGATTCTCTGTAAAAATATGTTATTAAGATGATTAATCTTTTATTATGTTTTGNCAAATATAAATNTTTGTGTTAGAAATGGT
TARGET=1243,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6828
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.678439
PRIMER_LEFT_SEQUENCE=ACGACGAGTTTCCATTGATCTT
PRIMER_RIGHT_SEQUENCE=CTAGGAACTCGGTAAAACCTCG
PRIMER_LEFT=1172,22
PRIMER_RIGHT=1283,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.322
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=112
PRIMER_PAIR_PENALTY_1=0.6828
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.678439
PRIMER_LEFT_1_SEQUENCE=AAGACGACGAGTTTCCATTGAT
PRIMER_RIGHT_1_SEQUENCE=CTAGGAACTCGGTAAAACCTCG
PRIMER_LEFT_1=1169,22
PRIMER_RIGHT_1=1283,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.322
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=0.7183
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.678439
PRIMER_LEFT_2_SEQUENCE=AACAAGACGACGAGTTTCCATT
PRIMER_RIGHT_2_SEQUENCE=CTAGGAACTCGGTAAAACCTCG
PRIMER_LEFT_2=1166,22
PRIMER_RIGHT_2=1283,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=59.322
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=0.8280
PRIMER_LEFT_3_PENALTY=0.149516
PRIMER_RIGHT_3_PENALTY=0.678439
PRIMER_LEFT_3_SEQUENCE=CCCGAAAGATAGGTGGTCTATG
PRIMER_RIGHT_3_SEQUENCE=CTAGGAACTCGGTAAAACCTCG
PRIMER_LEFT_3=1092,22
PRIMER_RIGHT_3=1283,22
PRIMER_LEFT_3_TM=59.850
PRIMER_RIGHT_3_TM=59.322
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=1.0676
PRIMER_LEFT_4_PENALTY=0.004359
PRIMER_RIGHT_4_PENALTY=1.063281
PRIMER_LEFT_4_SEQUENCE=ACGACGAGTTTCCATTGATCTT
PRIMER_RIGHT_4_SEQUENCE=ACTAGGAACTCGGTAAAACCTCG
PRIMER_LEFT_4=1172,22
PRIMER_RIGHT_4=1284,23
PRIMER_LEFT_4_TM=60.004
PRIMER_RIGHT_4_TM=60.063
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=113
=
PRIMER_SEQUENCE_ID=SSR_04186 Ps_contig_mira-and-tgicl-ass_franssen_32377
SEQUENCE=GTCGGGGGTCGATTGTGTGGCACATATCCCTTATTCCAAACTCTCTCACTTAACCGCTAAGCTTCCCTCTCTCGAACGAAGCATCCGCTTCCTATAAAAAACAAACTCCAAACTCATTTTCAAGGACTCATTTCAATTTCCATCATGGTGGTTGTTATTGAAGAAGAAGAAGGTGTTGAATTTCACGATCTCCCTGAAGGTTGCATCGAGAACGTTCTCTCGTTCACCACGCCGCGAGATGTTGCCAGACTCTCTTTGGTTTCGTCCACGTTCAAATCCGCCGCGGAATCCGATTCCGTTTGGGATAAATTCTTGCCGCCGGATTATCGTTCGATTATCTCTCAATCGGAAACGGATGTTGATGATTCTTTATCCGTTCTTTCTAAGAAGGATCTTTATATTAATCTTACGCAGAAACCGATTTTAATAGACGGTGGTAAAAAGAGCTTTCAATTGGATAAAGTTGATGGGAAGAAATGCTATATGTTATCGGCGAGGAGTTTGTTTATTGTATGGGGAGATACTCCTAGGTATTGGAAGTGGATTTCTCTTCCAGATTCAAGGTTTTCTGAAGTGGCTGAACTTGTGAGTGTGTGTTGGTTCGAAATCCGTGGCTGGATCAACACCAGTATGCTATCTCCACAGACTTCGTATGGAGCATATCTTGTATTCAAGGCAACTGGTTCTGGAACCTATGGGTTTGAATATCAGCCAGTTGAGGCCTCCATTGTCATTGCTGGAGGTGAACATGTTGAAAGAAATGTATTCTTGGATGTAGAAAGAGGTAGAAGGTTAAGATACCAAATCGTTCCTCGTCGTTTCCGTACTGGGATATTCAGTCGAGCACCTTTTGTGGAAGCACCAGTAGCACCCACAGAAAGTGTTGAAGATATGCAGAAGTATCCGAAGGATAGGGCAGATGGGTGGTTGGAGATGGAATTGGGAGAGTTCTTCAACGAAGGAGGAGATGATAAAGAGGTGGAGATAGGAGTTTATGAGGTGAAGGGTGGTGGTTGGAAGGGTGGTCTGGTTGTTCAAGGAATTGAAATAAGGCCTAAGCCTAAACCATGTTGAATGAGTGACTAAACCAAAAACTCAGGTTATTGTCATCTCAAAGTTTATGATTTTTGTATTAGGATCTGTTCTTGTATATGTCATCCTCATCTTCATGTTAGATATTACTGGTAAGAGTATGTTTCTTCCAATATGCAGTCAATGCGGATTATTAGCATCTCTGGTAAGAGGAATGTCACCACAATCAATGAGTATTGGGAGTGAGGGTGTTGTTTGTGTCTTTTTTACCTAGTTATATTTATTTAAATTAATAATTATAAATAAACTACATTGATAAATTTTCAATNGTTTTGTTTTNTGNAACGATTGATTTTCAAGTCTATAGTTGATAACTTTAGATGAGT
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1954
PRIMER_LEFT_PENALTY=0.042192
PRIMER_RIGHT_PENALTY=0.153161
PRIMER_LEFT_SEQUENCE=GGCACATATCCCTTATTCCAAA
PRIMER_RIGHT_SEQUENCE=GTGGACGAAACCAAAGAGAGTC
PRIMER_LEFT=18,22
PRIMER_RIGHT=268,22
PRIMER_LEFT_TM=60.042
PRIMER_RIGHT_TM=60.153
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.2167
PRIMER_LEFT_1_PENALTY=0.042192
PRIMER_RIGHT_1_PENALTY=0.174518
PRIMER_LEFT_1_SEQUENCE=GGCACATATCCCTTATTCCAAA
PRIMER_RIGHT_1_SEQUENCE=ACCAAAGAGAGTCTGGCAACAT
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=259,22
PRIMER_LEFT_1_TM=60.042
PRIMER_RIGHT_1_TM=60.175
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.2474
PRIMER_LEFT_2_PENALTY=0.042192
PRIMER_RIGHT_2_PENALTY=0.205226
PRIMER_LEFT_2_SEQUENCE=GGCACATATCCCTTATTCCAAA
PRIMER_RIGHT_2_SEQUENCE=AAACCAAAGAGAGTCTGGCAAC
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=261,22
PRIMER_LEFT_2_TM=60.042
PRIMER_RIGHT_2_TM=59.795
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.3232
PRIMER_LEFT_3_PENALTY=0.042192
PRIMER_RIGHT_3_PENALTY=0.281044
PRIMER_LEFT_3_SEQUENCE=GGCACATATCCCTTATTCCAAA
PRIMER_RIGHT_3_SEQUENCE=GACGAAACCAAAGAGAGTCTGG
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=265,22
PRIMER_LEFT_3_TM=60.042
PRIMER_RIGHT_3_TM=60.281
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=248
PRIMER_PAIR_PENALTY_4=0.3232
PRIMER_LEFT_4_PENALTY=0.042192
PRIMER_RIGHT_4_PENALTY=0.281044
PRIMER_LEFT_4_SEQUENCE=GGCACATATCCCTTATTCCAAA
PRIMER_RIGHT_4_SEQUENCE=GGACGAAACCAAAGAGAGTCTG
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=60.042
PRIMER_RIGHT_4_TM=60.281
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_04187 Ps_contig_mira-and-tgicl-ass_franssen_32379
SEQUENCE=GTCGGGGNACTCTCTTAAAATACATCCTCATCCTTTTCTTCTTNAGATCTCTTTCTCTTTCTCTTTTTCAACAATTCTCAATTTTCATCACTACCGTTAATCATTTATTTCCCAATGGCATCTAACAATCACCGCCGTTCAATCCCTGACATCAACGGCTCTCACCCCACTCCCAAAAAACCTAAACTCTCTTCACCTTCATCCTCCGCCATAACCGTTTCCGAAATTGAATCTGAATTCTCTCACCATGACGCCGCCGTCGCTCGCATCAACAACGGCAGTTTCGGTTCTTGTCCGTCCTCCGTCATCTCCGCTCAACAGAAATGGCAGCTCAAATACCTCCGTCAGCCTGACCACTTCTACTTCAACCACCTCAAACCGGCAATCGACACTTCACGTTCTATTATCAAAGACCTAGTCAACGCTCAACATCTCGATGAAATCTCTATTGTCGATAACGCTACCACTGCTGCTGCTATTGTTCTTCAACACACCGCTTGGTGTTTCCGTGAAGGGAAGTTCGACAAAGGTGACATTGTTGTTATGCTTCATTACGCTTACGGTGCTGTTAAGAAATCTATGGAGGCTTATGTCACTCGTGCTGGTGGAAAAGTCATTGAAGTTCCTCTCCCTTTTCCGGTGAGTTCTAATGAAGAGATTGTTACTGAGTTTAGAAAGGCTTTGGAGATAGGAAAAACGGATGGTAGAAAGGTTAGGTTAGCTGTCATTGATCATGTGACTTCTATGCCTTGTGTTGTGATTCCTGTTAAGGAGTTGATTCAGATTTGTAGAGAGGAAGGTGTGGAACAGGTTTTTGTTGATGCTGCTCATTCTATTGGGTGTACTGATGTTGACATGCAGGAGATTGGTGCTGATTATTACACTAGTAATTTGCACAAGTGGTTTTTTTGTCCGCCTTCGATTGCGTTTTTGTACTCGAGAAAAAACACTAAAACTACTGGTGGTGGTGGTGGTTTGCATCATCCTGTGGTGTCTCATGAGTATGGAAATGGGTTGGCTGTGGAAAGTGCTTGGATTGGGACAAGGGATTATAGTTCTCAATTGGTGGTCCCTGATGTTTTGGAATTTGTGAACAGGTTTGAAGGTGGTGTTGAAGGGATTAAGAAGAGGAATCATGAAGCTGTTGTTGAGATGGGAGAGATGCTGGTTAAGGCTTGGGGAACTCATCTTGGGAGTCCTTCACATATGTGTGCTAGTATGATTATGGTTGGTTTGCCTACTTGTTTGGGGATTCATAGTGATTCTGATGCTCTGAAGTTAAGGACACATTTGAGGGATGTTTTTGGAGTTGAAGTTCCTATATACTATAGACCACCTAGAGATGGAGAAGTAGAGCCTGTAACGGGGTATGCTCGGATTTCTCATCATGTTTACAATAAAGTCCAGGATTATTACAAGTTTAGGGATGCTGTTAACGAACTTGTGGATAATGGGTTTGATTGTACTCTTCTTTCCAACTGAAGAGCTGAGAAGTGTCTTTGTCGGCTTTCTGATGAATAAAAGCAATGGTTGTTGTAATTGCTGCTAGCTTGCTTGCAATGTTTGCTAGTTACGATGGTTTTGGTACCATGAACTGCATGTTTTGCAGGTAAACTTCAGCAATTACCCATGTTATCTTTGCTGAGTAGAATCCCTTAGGGAGTGAGCAACTTCAATAGTGAGATCTATGGATTTATCCTTGTAAATATAAATGTGATTCTCTCATGGTGTAATGATATTCACTCTCATTGTGCCGAGTTGCCAACTGCTTCTTCAGGTTTATAACAAAGTTCTACCATAAA
TARGET=960,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1196
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.033245
PRIMER_LEFT_SEQUENCE=GTGTGGAACAGGTTTTTGTTGA
PRIMER_RIGHT_SEQUENCE=ACTCATGAGACACCACAGGATG
PRIMER_LEFT=799,22
PRIMER_RIGHT=1002,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=0.1866
PRIMER_LEFT_1_PENALTY=0.141511
PRIMER_RIGHT_1_PENALTY=0.045112
PRIMER_LEFT_1_SEQUENCE=CTGATGTTGACATGCAGGAGAT
PRIMER_RIGHT_1_SEQUENCE=CACCTTCAAACCTGTTCACAAA
PRIMER_LEFT_1=844,22
PRIMER_RIGHT_1=1107,22
PRIMER_LEFT_1_TM=60.142
PRIMER_RIGHT_1_TM=60.045
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.2224
PRIMER_LEFT_2_PENALTY=0.086339
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=GTGTGGAACAGGTTTTTGTTGA
PRIMER_RIGHT_2_SEQUENCE=AGGGACCACCAATTGAGAACTA
PRIMER_LEFT_2=799,22
PRIMER_RIGHT_2=1073,22
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.2228
PRIMER_LEFT_3_PENALTY=0.189529
PRIMER_RIGHT_3_PENALTY=0.033245
PRIMER_LEFT_3_SEQUENCE=AAGGTGTGGAACAGGTTTTTGT
PRIMER_RIGHT_3_SEQUENCE=ACTCATGAGACACCACAGGATG
PRIMER_LEFT_3=796,22
PRIMER_RIGHT_3=1002,22
PRIMER_LEFT_3_TM=59.810
PRIMER_RIGHT_3_TM=60.033
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=0.2228
PRIMER_LEFT_4_PENALTY=0.189529
PRIMER_RIGHT_4_PENALTY=0.033245
PRIMER_LEFT_4_SEQUENCE=AGGTGTGGAACAGGTTTTTGTT
PRIMER_RIGHT_4_SEQUENCE=ACTCATGAGACACCACAGGATG
PRIMER_LEFT_4=797,22
PRIMER_RIGHT_4=1002,22
PRIMER_LEFT_4_TM=59.810
PRIMER_RIGHT_4_TM=60.033
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=206
=
PRIMER_SEQUENCE_ID=SSR_04188 Ps_contig_mira-and-tgicl-ass_franssen_32380
SEQUENCE=TCACGACAGAGTACGCGGGCGGCTCTCTTCTTCTCTCTCTCTCTGAAACTAGTTACTTATTATTCTCAARRANGTGTGTTGTTGTCTACAAATGAAGGATTTGGATTTGTGCATTTGAGATATTCATCTAGGTATATGGAGAAGAAAGAGATGGCTGTGATGAGTGTGAAGAGTGAAGAAGATGTTGTTGTTGGTGTTGGTGGTGGTGGTTCTTCATCTTTGCTAAACGGTTTTCAGTTTTCAAGCGTGTTTGATTTCTATGAGGTAGAAAAAGGCTCTTCTTTAGGGTTTATGGAGTTACTGGGTGTGGAAAACTATAGTTCTTTGCTTGATGTACCGCAACTATCAACCATGAGTCATCATCAAACTACAGTTAAGTCTTCATCTGATACTAATGGAAAAGAGTGTTCTGAGGTTTTGAATCATCTTCCTCAAACTCCTAACTCTTGCTCGATTTCATCGGCCTCTAGTGAAGCAGTTAATGATGAACAACATAATAAGATTCTTGTTGACCAAGTCCAAGAAGATGAAGATGAAGAAGAAGAAGAAAAACAAAAAACCAGCAAACAGTTGAAGACTAAGAAGACAAATCAAAAGAGACAGAGAGAACCAAGATTCGCGTTCATGACGAAAAGCGAGGTGGATCATCTGGAAGATGGGTACAGATGGAGAAAGTACGGTCAAAAAGCTGTCAAAAACAGCCCCTTTCCGAGGAGCTATTATCGCTGCACCACTGTTTCATGTAATGTGAAGAAACGTGTGGAGCGGTCTTATACTGATCCAACCATTGTAGTCACAACTTATGAAGGCCAACACATTCATCCTAGTCCAGCTATGTCCCGTTCCGCCTTCGCCGGAGTTCAGATACCACCGCCGAACGCTGTATCCGGTGGGTTTCCAACAAACTTTGGTTCAGTTTTGCAAGGGAACTACTTGTCTCAATATCAGCAGCAACAACACCATCAACATCAACATCAACAGCTACTGGTTAATACATTGTCCTCTTTGAGTCACCCTTACCACCAAGAGAGGAAGCTTTGTAATCCAGGGACAAGTTCCTTTCTTAGGGACCATGGTCTTCTTCAAGATGTTGTTCCTTCACATATGTTGAAAGAAGAGTAAATATGATTTGATAAATAGCTTCATTATTACTTTCTATGTTAGTATTTCTTTCTTTTTAGTATATTTTCTTGCTGACTTTTTCTGTAAGACTTAGGTAACAACTATGTAACTTTTCTTTCTGATATGACGGATAGGTGGTGAGGGTGATATATTACTATTAGTGATGAATGATAATCATCATCTTGTAAAGACCACTTTATCTTTATTGCATATAGCCCACATCATGATTATTATGATCTAATAAAATANGATATCAACNCTCAATTAAATTACTTACCAAAACAAAAAAATTAAAA
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04189 Ps_contig_mira-and-tgicl-ass_franssen_32380
SEQUENCE=TCACGACAGAGTACGCGGGCGGCTCTCTTCTTCTCTCTCTCTCTGAAACTAGTTACTTATTATTCTCAARRANGTGTGTTGTTGTCTACAAATGAAGGATTTGGATTTGTGCATTTGAGATATTCATCTAGGTATATGGAGAAGAAAGAGATGGCTGTGATGAGTGTGAAGAGTGAAGAAGATGTTGTTGTTGGTGTTGGTGGTGGTGGTTCTTCATCTTTGCTAAACGGTTTTCAGTTTTCAAGCGTGTTTGATTTCTATGAGGTAGAAAAAGGCTCTTCTTTAGGGTTTATGGAGTTACTGGGTGTGGAAAACTATAGTTCTTTGCTTGATGTACCGCAACTATCAACCATGAGTCATCATCAAACTACAGTTAAGTCTTCATCTGATACTAATGGAAAAGAGTGTTCTGAGGTTTTGAATCATCTTCCTCAAACTCCTAACTCTTGCTCGATTTCATCGGCCTCTAGTGAAGCAGTTAATGATGAACAACATAATAAGATTCTTGTTGACCAAGTCCAAGAAGATGAAGATGAAGAAGAAGAAGAAAAACAAAAAACCAGCAAACAGTTGAAGACTAAGAAGACAAATCAAAAGAGACAGAGAGAACCAAGATTCGCGTTCATGACGAAAAGCGAGGTGGATCATCTGGAAGATGGGTACAGATGGAGAAAGTACGGTCAAAAAGCTGTCAAAAACAGCCCCTTTCCGAGGAGCTATTATCGCTGCACCACTGTTTCATGTAATGTGAAGAAACGTGTGGAGCGGTCTTATACTGATCCAACCATTGTAGTCACAACTTATGAAGGCCAACACATTCATCCTAGTCCAGCTATGTCCCGTTCCGCCTTCGCCGGAGTTCAGATACCACCGCCGAACGCTGTATCCGGTGGGTTTCCAACAAACTTTGGTTCAGTTTTGCAAGGGAACTACTTGTCTCAATATCAGCAGCAACAACACCATCAACATCAACATCAACAGCTACTGGTTAATACATTGTCCTCTTTGAGTCACCCTTACCACCAAGAGAGGAAGCTTTGTAATCCAGGGACAAGTTCCTTTCTTAGGGACCATGGTCTTCTTCAAGATGTTGTTCCTTCACATATGTTGAAAGAAGAGTAAATATGATTTGATAAATAGCTTCATTATTACTTTCTATGTTAGTATTTCTTTCTTTTTAGTATATTTTCTTGCTGACTTTTTCTGTAAGACTTAGGTAACAACTATGTAACTTTTCTTTCTGATATGACGGATAGGTGGTGAGGGTGATATATTACTATTAGTGATGAATGATAATCATCATCTTGTAAAGACCACTTTATCTTTATTGCATATAGCCCACATCATGATTATTATGATCTAATAAAATANGATATCAACNCTCAATTAAATTACTTACCAAAACAAAAAAATTAAAA
TARGET=198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04190 Ps_contig_mira-and-tgicl-ass_franssen_32380
SEQUENCE=TCACGACAGAGTACGCGGGCGGCTCTCTTCTTCTCTCTCTCTCTGAAACTAGTTACTTATTATTCTCAARRANGTGTGTTGTTGTCTACAAATGAAGGATTTGGATTTGTGCATTTGAGATATTCATCTAGGTATATGGAGAAGAAAGAGATGGCTGTGATGAGTGTGAAGAGTGAAGAAGATGTTGTTGTTGGTGTTGGTGGTGGTGGTTCTTCATCTTTGCTAAACGGTTTTCAGTTTTCAAGCGTGTTTGATTTCTATGAGGTAGAAAAAGGCTCTTCTTTAGGGTTTATGGAGTTACTGGGTGTGGAAAACTATAGTTCTTTGCTTGATGTACCGCAACTATCAACCATGAGTCATCATCAAACTACAGTTAAGTCTTCATCTGATACTAATGGAAAAGAGTGTTCTGAGGTTTTGAATCATCTTCCTCAAACTCCTAACTCTTGCTCGATTTCATCGGCCTCTAGTGAAGCAGTTAATGATGAACAACATAATAAGATTCTTGTTGACCAAGTCCAAGAAGATGAAGATGAAGAAGAAGAAGAAAAACAAAAAACCAGCAAACAGTTGAAGACTAAGAAGACAAATCAAAAGAGACAGAGAGAACCAAGATTCGCGTTCATGACGAAAAGCGAGGTGGATCATCTGGAAGATGGGTACAGATGGAGAAAGTACGGTCAAAAAGCTGTCAAAAACAGCCCCTTTCCGAGGAGCTATTATCGCTGCACCACTGTTTCATGTAATGTGAAGAAACGTGTGGAGCGGTCTTATACTGATCCAACCATTGTAGTCACAACTTATGAAGGCCAACACATTCATCCTAGTCCAGCTATGTCCCGTTCCGCCTTCGCCGGAGTTCAGATACCACCGCCGAACGCTGTATCCGGTGGGTTTCCAACAAACTTTGGTTCAGTTTTGCAAGGGAACTACTTGTCTCAATATCAGCAGCAACAACACCATCAACATCAACATCAACAGCTACTGGTTAATACATTGTCCTCTTTGAGTCACCCTTACCACCAAGAGAGGAAGCTTTGTAATCCAGGGACAAGTTCCTTTCTTAGGGACCATGGTCTTCTTCAAGATGTTGTTCCTTCACATATGTTGAAAGAAGAGTAAATATGATTTGATAAATAGCTTCATTATTACTTTCTATGTTAGTATTTCTTTCTTTTTAGTATATTTTCTTGCTGACTTTTTCTGTAAGACTTAGGTAACAACTATGTAACTTTTCTTTCTGATATGACGGATAGGTGGTGAGGGTGATATATTACTATTAGTGATGAATGATAATCATCATCTTGTAAAGACCACTTTATCTTTATTGCATATAGCCCACATCATGATTATTATGATCTAATAAAATANGATATCAACNCTCAATTAAATTACTTACCAAAACAAAAAAATTAAAA
TARGET=535,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04191 Ps_contig_mira-and-tgicl-ass_franssen_32381
SEQUENCE=CATCAAATGGGCATCACGGTGGGACACATATCTTCTAATGAATGATGATCAAATCCACTGGTTCTCCATCATTAACTCTCTGATGATTGTTCTTTTCCTTTCTGGAATGGTGGCAATGATCATGATGAGAACTCTTTACAGAGACATTGCCAATTACAACCAGCTAGAAACCCAAGATGAGGCCCAGGAAGAAACAGGGTGGAAACTTGTTCATGGAGATGTTTTCAGGCCACCAATCAATTCAAATTTGCTTTGTGTTTATGTTGGTACTGGTGTTCAGATCTTTGGAATGACACTTGTTACAATGATATTTGCTTTGCTAGGGTTCTTGTCTCCATCTAATCGTGGAGGTCTCATGACTGCAATGGTTCTACTATGGGTTTTTATGGGTTTATTTGCTGGCTACTCCTCGGCACGTTTGTACAAGATGTTCAAGGGCACAGAGTGGAAGAGGAATACCTTAAAAACCGCGTTTATGTTTCCGGGCATTCTTTTTGCCATCTTCTTTGTGCTGAATGCTTTGATCTGGGGGGAGCAGTCCTCTGGGGCAGTCCCCTTTGGAACAATGATTGCTCTAGTGTGCTTATGGTTTGGTATATCGGTACCCTTGGTCTTTGTGGGCAGTTACCTGGGTTTTAAAAAGCCACAAATTGAAGACCCTGTCAAGACTAATAAAATTCCTAGGCAGGTACCTGAGCAGGCATGGTACATGACACCTGTCTTCTCAATTCTGATTGGTGGCATTCTTCCCTTTGGTGCAGTGTTTATCGAGCTCTTCTTTATCCTGACATCAATATGGCTGAACCAGTTCTACTACATCTTTGGATTCCTTTTCATAGTGTTCATCATCCTATTGATCACATGTGCAGAGATTACGGTCGTGCTTTGCTACTTCCAGTTGTGCAGTGAGGACTACAATTGGTGGTGGAGATCTTACCTCACTGCCGGGTCATCGGCTGTTTACCTTTTCCTCTACTCAATTTTCTACTTCTTTACCAAATTGGAGATTACAAAGCTGGTCTCAGGCATTCTTTACTTTGGGTATATGATTATTGTTTCATATGCCTTCTTTGTTGTGACTGGAACTATTGGATTCTACGCTTGCTTCTGGTTTGTCCGCAAGATCTACTCTTCTGTGAAGATTGATTGATTACAAGCATCATATAGCGGATGGAACTTGAGTGACTGTAGGATTTCGAGGACTGGAAGCAACTACCGTTGGGAAGTTCTCTTTCCCCCTAGTCTTGAAAACCCATTTTGTTTATTGTTAGGGTAGCAATTTACTGTTAATTTGTTGCACTGTTTTCAAATATTGTTGTGGTAGTTTCAAAAGTAATGATGAGATGTCTGCATGTTTAATGTAGTAGTGATGATATTCTAGATCAAACTAATTAGTCTTGTACAAGGAATGTTTCACATTARGAACAAATGAGATATCTGCATATTTAAC
TARGET=1141,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04192 Ps_contig_mira-and-tgicl-ass_franssen_32383
SEQUENCE=TCGTCGGCCACCAGGAAGAAAAGGCTCATTAGTAGAAAAATGGCTACCGAAGAAAACAAAAGAATCTTGTTTGAAATCTTGGAAAATTTGAAATTAATCGATGTGGCTAAATATATGAACTTTGTGAGTGCTCCACAAGCTGGTGCTATAGCTACATTTTCAGGCACAACTCRTGACACTTTTGACGGAAAAGCAGTTTTGGAGTTGATATATGAAGCTTACATTCCAATGGCGATACGTTGTATCAAGTCTGTCTGTTCATCAGCAAGAGAGTCCTGGAGTCTACATTCCATTGCTGTTGCTCATCGGCTAGGTACAGTACCTGTTGGAGAAACGAGTATCTTCATCGCAGTGTCGTCTGTTCATAGGGCTGATGCACTTGAGGCATGTAAATATTTGATAGATGAGGTAAAGGCAACCGTTCCTATTTGGAAAAAGGAGGTTTATTCAAATGGAGAGGTATGGAAAGAGAACACTGAATTCTTGGAGAGAAGGTCTGAGCTTGGTAAAAAGGATGTAGCTGTGGCTTGCTATGGGAAAAAGTTAGAAAATAAGGAGCATAAGTAAAAAGCATTGTTGTGGGAATAAGATTCGGATTGACGAAGGTAGCCAAGATTAAAATTGTATTTAAGCTTTGAAGTCAATAAACCTGCCCATCTGTCGACACTAATTTATCAATCTTGTTATTTAAGTTCTCTCATTTAGAATTCTCATCAGAAAAGAAAAATAAATAAATTCCACCAGCAATAAC
TARGET=725,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04193 Ps_contig_mira-and-tgicl-ass_franssen_32403
SEQUENCE=CGGGGTTTTTAACTTTTTTCTTTTTAACCTTTTTTCTTTAGTTTGCAACAACTAAACCATTATTCATTCCTCATATTCCCATTAACAAATGTAACAATTGTTCCTTCAACAACAACAACAAAAATGGAAAATCGTCCAAGAATAACACTCTATGATCAAATGACTTCATCCAGTAACACAACAAGCAACACTCGAAGCTCACTTGCAACGTTAATCCAAAGCGACGCCGTTTTCGCCATTCAGAAAACCCGAAACCAAACTCTCTTAGACATTATACGAGAAAACGAACCCAACAACGTTGTTGATAACAGCAACAATACAAAAGATCGAAAATCGTGGAAAGCTTTTAAGGAATTACTTCGTCTAAAGCGTCGTAACTCGGACGAGTCAACTCAACAACAACAACAACAAAACGACGTCGTTCGGGAAGATCTGAACAATTCGGATCCGGTCGAATTGCTCGCCGGCGGGGAGTCCTCCGACGAGGAAATGAACGTTAATAGCTCGGAGACGGCGCAGGTTAGTATGTCGCTGATGGATTTGTTAGAAGAGAGTGAAATTGATTTGGATCGAATTGGTGATGTTGACGAAGGTGATGACGTGGATGAGAAACGAGAAGAAGAAGAAGGGAATGTTTCGGTGGAACGCAATTGCTGCGTGTGTATGGTGAGGCATAAAGGTGCAGCGTTTATACCGTGTGGACACACGTTTTGCAGGATGTGTTCAAGAGAACTATGGGTGAGTAGAGGAAATTGTCCTCTTTGCAATAATTTGATATTGGAAGTTCTTGATTATTTTCTAATTTTCTCTCTNAAAATTTTTTTAAAAANTTTNGTAAGTATTTAACTTTTTCCCCTATTATTTATCTCTCTGTANTNTGTTGAGTTGGT
TARGET=107,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04194 Ps_contig_mira-and-tgicl-ass_franssen_32403
SEQUENCE=CGGGGTTTTTAACTTTTTTCTTTTTAACCTTTTTTCTTTAGTTTGCAACAACTAAACCATTATTCATTCCTCATATTCCCATTAACAAATGTAACAATTGTTCCTTCAACAACAACAACAAAAATGGAAAATCGTCCAAGAATAACACTCTATGATCAAATGACTTCATCCAGTAACACAACAAGCAACACTCGAAGCTCACTTGCAACGTTAATCCAAAGCGACGCCGTTTTCGCCATTCAGAAAACCCGAAACCAAACTCTCTTAGACATTATACGAGAAAACGAACCCAACAACGTTGTTGATAACAGCAACAATACAAAAGATCGAAAATCGTGGAAAGCTTTTAAGGAATTACTTCGTCTAAAGCGTCGTAACTCGGACGAGTCAACTCAACAACAACAACAACAAAACGACGTCGTTCGGGAAGATCTGAACAATTCGGATCCGGTCGAATTGCTCGCCGGCGGGGAGTCCTCCGACGAGGAAATGAACGTTAATAGCTCGGAGACGGCGCAGGTTAGTATGTCGCTGATGGATTTGTTAGAAGAGAGTGAAATTGATTTGGATCGAATTGGTGATGTTGACGAAGGTGATGACGTGGATGAGAAACGAGAAGAAGAAGAAGGGAATGTTTCGGTGGAACGCAATTGCTGCGTGTGTATGGTGAGGCATAAAGGTGCAGCGTTTATACCGTGTGGACACACGTTTTGCAGGATGTGTTCAAGAGAACTATGGGTGAGTAGAGGAAATTGTCCTCTTTGCAATAATTTGATATTGGAAGTTCTTGATTATTTTCTAATTTTCTCTCTNAAAATTTTTTTAAAAANTTTNGTAAGTATTTAACTTTTTCCCCTATTATTTATCTCTCTGTANTNTGTTGAGTTGGT
TARGET=394,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5750
PRIMER_LEFT_PENALTY=0.371677
PRIMER_RIGHT_PENALTY=0.203283
PRIMER_LEFT_SEQUENCE=AAACCCGAAACCAAACTCTCTT
PRIMER_RIGHT_SEQUENCE=CATCAGCGACATACTAACCTGC
PRIMER_LEFT=244,22
PRIMER_RIGHT=536,22
PRIMER_LEFT_TM=60.372
PRIMER_RIGHT_TM=59.797
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.5750
PRIMER_LEFT_1_PENALTY=0.371677
PRIMER_RIGHT_1_PENALTY=0.203283
PRIMER_LEFT_1_SEQUENCE=AAAACCCGAAACCAAACTCTCT
PRIMER_RIGHT_1_SEQUENCE=CATCAGCGACATACTAACCTGC
PRIMER_LEFT_1=243,22
PRIMER_RIGHT_1=536,22
PRIMER_LEFT_1_TM=60.372
PRIMER_RIGHT_1_TM=59.797
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=0.5750
PRIMER_LEFT_2_PENALTY=0.371677
PRIMER_RIGHT_2_PENALTY=0.203283
PRIMER_LEFT_2_SEQUENCE=AGAAAACCCGAAACCAAACTCT
PRIMER_RIGHT_2_SEQUENCE=CATCAGCGACATACTAACCTGC
PRIMER_LEFT_2=241,22
PRIMER_RIGHT_2=536,22
PRIMER_LEFT_2_TM=60.372
PRIMER_RIGHT_2_TM=59.797
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=0.7010
PRIMER_LEFT_3_PENALTY=0.165541
PRIMER_RIGHT_3_PENALTY=0.535412
PRIMER_LEFT_3_SEQUENCE=CTCACTTGCAACGTTAATCCAA
PRIMER_RIGHT_3_SEQUENCE=AATTGTTCAGATCTTCCCGAAC
PRIMER_LEFT_3=197,22
PRIMER_RIGHT_3=441,22
PRIMER_LEFT_3_TM=60.166
PRIMER_RIGHT_3_TM=59.465
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.7174
PRIMER_LEFT_4_PENALTY=0.371677
PRIMER_RIGHT_4_PENALTY=0.345718
PRIMER_LEFT_4_SEQUENCE=AAACCCGAAACCAAACTCTCTT
PRIMER_RIGHT_4_SEQUENCE=CCATCAGCGACATACTAACCTG
PRIMER_LEFT_4=244,22
PRIMER_RIGHT_4=537,22
PRIMER_LEFT_4_TM=60.372
PRIMER_RIGHT_4_TM=59.654
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_04195 Ps_contig_mira-and-tgicl-ass_franssen_32403
SEQUENCE=CGGGGTTTTTAACTTTTTTCTTTTTAACCTTTTTTCTTTAGTTTGCAACAACTAAACCATTATTCATTCCTCATATTCCCATTAACAAATGTAACAATTGTTCCTTCAACAACAACAACAAAAATGGAAAATCGTCCAAGAATAACACTCTATGATCAAATGACTTCATCCAGTAACACAACAAGCAACACTCGAAGCTCACTTGCAACGTTAATCCAAAGCGACGCCGTTTTCGCCATTCAGAAAACCCGAAACCAAACTCTCTTAGACATTATACGAGAAAACGAACCCAACAACGTTGTTGATAACAGCAACAATACAAAAGATCGAAAATCGTGGAAAGCTTTTAAGGAATTACTTCGTCTAAAGCGTCGTAACTCGGACGAGTCAACTCAACAACAACAACAACAAAACGACGTCGTTCGGGAAGATCTGAACAATTCGGATCCGGTCGAATTGCTCGCCGGCGGGGAGTCCTCCGACGAGGAAATGAACGTTAATAGCTCGGAGACGGCGCAGGTTAGTATGTCGCTGATGGATTTGTTAGAAGAGAGTGAAATTGATTTGGATCGAATTGGTGATGTTGACGAAGGTGATGACGTGGATGAGAAACGAGAAGAAGAAGAAGGGAATGTTTCGGTGGAACGCAATTGCTGCGTGTGTATGGTGAGGCATAAAGGTGCAGCGTTTATACCGTGTGGACACACGTTTTGCAGGATGTGTTCAAGAGAACTATGGGTGAGTAGAGGAAATTGTCCTCTTTGCAATAATTTGATATTGGAAGTTCTTGATTATTTTCTAATTTTCTCTCTNAAAATTTTTTTAAAAANTTTNGTAAGTATTTAACTTTTTCCCCTATTATTTATCTCTCTGTANTNTGTTGAGTTGGT
TARGET=615,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3104
PRIMER_LEFT_PENALTY=0.193120
PRIMER_RIGHT_PENALTY=0.117281
PRIMER_LEFT_SEQUENCE=GGACGAGTCAACTCAACAACAA
PRIMER_RIGHT_SEQUENCE=CCTTTATGCCTCACCATACACA
PRIMER_LEFT=380,22
PRIMER_RIGHT=679,22
PRIMER_LEFT_TM=60.193
PRIMER_RIGHT_TM=59.883
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.3206
PRIMER_LEFT_1_PENALTY=0.203283
PRIMER_RIGHT_1_PENALTY=0.117281
PRIMER_LEFT_1_SEQUENCE=GCAGGTTAGTATGTCGCTGATG
PRIMER_RIGHT_1_SEQUENCE=CCTTTATGCCTCACCATACACA
PRIMER_LEFT_1=515,22
PRIMER_RIGHT_1=679,22
PRIMER_LEFT_1_TM=59.797
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=0.3206
PRIMER_LEFT_2_PENALTY=0.203283
PRIMER_RIGHT_2_PENALTY=0.117281
PRIMER_LEFT_2_SEQUENCE=GCAGGTTAGTATGTCGCTGATG
PRIMER_RIGHT_2_SEQUENCE=CACCTTTATGCCTCACCATACA
PRIMER_LEFT_2=515,22
PRIMER_RIGHT_2=681,22
PRIMER_LEFT_2_TM=59.797
PRIMER_RIGHT_2_TM=59.883
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.3573
PRIMER_LEFT_3_PENALTY=0.203283
PRIMER_RIGHT_3_PENALTY=0.154023
PRIMER_LEFT_3_SEQUENCE=GCAGGTTAGTATGTCGCTGATG
PRIMER_RIGHT_3_SEQUENCE=TCTTGAACACATCCTGCAAAAC
PRIMER_LEFT_3=515,22
PRIMER_RIGHT_3=728,22
PRIMER_LEFT_3_TM=59.797
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.3935
PRIMER_LEFT_4_PENALTY=0.276218
PRIMER_RIGHT_4_PENALTY=0.117281
PRIMER_LEFT_4_SEQUENCE=GAACGTTAATAGCTCGGAGACG
PRIMER_RIGHT_4_SEQUENCE=CCTTTATGCCTCACCATACACA
PRIMER_LEFT_4=491,22
PRIMER_RIGHT_4=679,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=59.883
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_04196 Ps_contig_mira-and-tgicl-ass_franssen_32408
SEQUENCE=TTTTANTNGTTAAACAAATGGTTCAACTCACATTAAAAAATTATAGTTGATTCATATAGTTGGATTAAACAAATGGTAGTATTTTAATATCTAAAGAAAATACTGGTAAGCATTCTAAAAGCTTGTGTGTACTTTTCTCCTGGGGTTGAAGTTGAACATGTAATATACAAGAACACATCAGACACAAAATGCCTGGCATGGATAGGGTTGCACTAAAGCTTAACAAATCAATCATATCTGCTTCTTCTCGTACAACCTTCGATAAATTTTCGCTCCTTCGAATTCTGCATGAATCTCTCTCTCAGTCCTAATCCCCATAGGGTTGCTTAATAACCATCCTTCACTGTCACACAATGTAGCAGGGTTCAAAGCATCTATGTGACTTAGTGCATCAACTTCATCAAACTGGTTTTTCATGTCAAGTGCCACTTCAACCACATCAGACTGTATGAATACCTGTCCTCCAGGAGTTAAATTGTCTACAATAGCACCAACCAAAGGCTTCTGCAGAACTCTTCTTTTATGATGTTTTTTCTTGAAATGAGGATCTGGACACAAAATTGAAACTAACTGCAATGGCCCGGGATATGATTCTACCAACTGCTTGAAAGAAATTGGGGCATTTGCAAACAAGAAATGTATGTTATCTAGAGCCATGTCTTTTACCCATGACTCAGCACGCTTGACCATCCTCTGCCTTATTTCCAATCCCAAATAATTTCTCACTTTAGGTGTCCTTTTTGCAAGCCACATAAGAAACCTGCCACTGCCACATCCAATATCTACCATGAGTGGCAATGCAGGGTCTGCAAAAACCTGGTTCCAGTCTGGTACTTGTGCAGGAGTAGCGAAAGAAGAACTGAGGGGATTAACATGTTGCCTGATTCTGACATGACCTAGTTCCAAATTGTTAGAGAGGTTGAGGTCAGCGTATTCCAATGCTACGAGTTGCGAGCTTCGTTTTGATTGTTGTTGGTCTTGTTCTAGTTGGGAAATGGAGGAAGAAGAAGATGAAGGGTGGGAATTGGAAGAGAATGGAAGAGAAGAAGAGAGAGGACGAAAGGGTGAGAGTGGTAACATTGGTGTAGAAAGTGAACTACCAATGCCATACAACTTCTTCCAACCGCAACAACCCATTCATTCCTCCTTGCGCTTCAATCCCTCGCCTAAACTATCTGCTTTATTTGTNCT
TARGET=294,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1011
PRIMER_LEFT_PENALTY=0.095523
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=AACACATCAGACACAAAATGCC
PRIMER_RIGHT_SEQUENCE=ACTCCTGGAGGACAGGTATTCA
PRIMER_LEFT=171,22
PRIMER_RIGHT=470,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.1232
PRIMER_LEFT_1_PENALTY=0.095523
PRIMER_RIGHT_1_PENALTY=0.027715
PRIMER_LEFT_1_SEQUENCE=AACACATCAGACACAAAATGCC
PRIMER_RIGHT_1_SEQUENCE=ATGCTTTGAACCCTGCTACATT
PRIMER_LEFT_1=171,22
PRIMER_RIGHT_1=373,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.1270
PRIMER_LEFT_2_PENALTY=0.099308
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=GTGTACTTTTCTCCTGGGGTTG
PRIMER_RIGHT_2_SEQUENCE=ATGCTTTGAACCCTGCTACATT
PRIMER_LEFT_2=126,22
PRIMER_RIGHT_2=373,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=0.1270
PRIMER_LEFT_3_PENALTY=0.099308
PRIMER_RIGHT_3_PENALTY=0.027715
PRIMER_LEFT_3_SEQUENCE=TGTGTACTTTTCTCCTGGGGTT
PRIMER_RIGHT_3_SEQUENCE=ATGCTTTGAACCCTGCTACATT
PRIMER_LEFT_3=125,22
PRIMER_RIGHT_3=373,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=60.028
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.1951
PRIMER_LEFT_4_PENALTY=0.099308
PRIMER_RIGHT_4_PENALTY=0.095831
PRIMER_LEFT_4_SEQUENCE=GTGTACTTTTCTCCTGGGGTTG
PRIMER_RIGHT_4_SEQUENCE=CCCTGCTACATTGTGTGACAGT
PRIMER_LEFT_4=126,22
PRIMER_RIGHT_4=363,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_04197 Ps_contig_mira-and-tgicl-ass_franssen_32419
SEQUENCE=AAGTTGGTTCGGAAAAACCCTAAACGGTGGAGTAGTCGAGTTTGAGTTGAGTTCAGGGTTTACTTTCAATTTGAATTTCAATTCAGTACCAACTAAACCTATTGACTCTCTCTCTCTCTCTCTCTCTCTCTGCAATGGCAGTTTCAACGGAATTTCACCTTCCACGTCTCTCCGTCGTCGACTTTCCCTCTCTTCTCCAAGCTATGGCCGCCGACACTCTTCTTGCCGCTGCTCAATCTCTCGCTCTCATTGGATATCTGCTGGCTAATTTCACCAGTTTAGTGTCTCCAAACTTGACCGCAATGGATTCAAGGATTCCTTTAGAACACCTTTTAGGTAGGAAACCTGCATATTTGAAAAACAGCAGTGATACAGAGGATGACGACGATGACGACGATGACGAGGATGTACAAGATGAGGACGACGATGGAGAAGACGAAGACGTCTCAGGTGACGAAGGCGAAGAAGAAGGGGATCCTGAGGATGATCCTGAGGCTAATGGTGCAGGAGGAAGTGACAATGACGACGATGGTGACGACGATGGCGACGATGGTGACGAAGAAGATGAAGAAGATGGCGAAGATGAAGATGAAGAGGATGAAGACGAGGAAGATGATGATACACCCCAGCAGCCACCAACTAAGAAGAGGAAGTGAGAATTATATTAGTAGGAAATTGGTTCCATGGGTTGCATTTGAGTTAAGCTTAGTGACTAGGAAAGATACATTAGCAACCTTCGTGAGGAAACCTGTGTTGCTTTATTTTATGTCAATGATGATTATTGTTACTAGTATATTGATAATTCTCTTTGTTTCTATTTTAAGTTCTTCTGTCCAGTTATTATCANAAAACG
TARGET=106,26
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2014
PRIMER_LEFT_PENALTY=0.177853
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=CCTAAACGGTGGAGTAGTCGAG
PRIMER_RIGHT_SEQUENCE=CATAGCTTGGAGAAGAGAGGGA
PRIMER_LEFT=18,22
PRIMER_RIGHT=205,22
PRIMER_LEFT_TM=60.178
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.4358
PRIMER_LEFT_1_PENALTY=0.177853
PRIMER_RIGHT_1_PENALTY=0.257927
PRIMER_LEFT_1_SEQUENCE=CCTAAACGGTGGAGTAGTCGAG
PRIMER_RIGHT_1_SEQUENCE=TGGTGAAATTAGCCAGCAGATA
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=275,22
PRIMER_LEFT_1_TM=60.178
PRIMER_RIGHT_1_TM=59.742
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.4737
PRIMER_LEFT_2_PENALTY=0.177853
PRIMER_RIGHT_2_PENALTY=0.295825
PRIMER_LEFT_2_SEQUENCE=CCTAAACGGTGGAGTAGTCGAG
PRIMER_RIGHT_2_SEQUENCE=TTAGCCAGCAGATATCCAATGA
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=60.178
PRIMER_RIGHT_2_TM=59.704
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.5087
PRIMER_LEFT_3_PENALTY=0.485189
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=TCGAGTTTGAGTTGAGTTCAGG
PRIMER_RIGHT_3_SEQUENCE=CATAGCTTGGAGAAGAGAGGGA
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=59.515
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.5467
PRIMER_LEFT_4_PENALTY=0.177853
PRIMER_RIGHT_4_PENALTY=0.368866
PRIMER_LEFT_4_SEQUENCE=CCTAAACGGTGGAGTAGTCGAG
PRIMER_RIGHT_4_SEQUENCE=GCTTGGAGAAGAGAGGGAAAGT
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=201,22
PRIMER_LEFT_4_TM=60.178
PRIMER_RIGHT_4_TM=60.369
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_04198 Ps_contig_mira-and-tgicl-ass_franssen_32421
SEQUENCE=CAAGGCCCAGTCATATGAGAGGGAAGCGATGGTATATCAGCAGGAGAAGAACCATCTTGAGCAGAAGTACCGATCTGAGTTCAAAAGATTTGAGGAGGTTCAAGAAAGGTGTAAAAGGGCTGAAAAAGAAGCTGCAAGGTCTACTGAAGTGGCTGATAGAGCACGGGCTGAAGCAGGTACGGCTCTGGAGGAGAAGAATAAGATGCAGAGTGTGGCAATGGAAAGACTGGCAGAGATTGAGAGGGCTGAGAGGAAAATTGAAACTCTTGGGAGGGAGAAAGATAATCTGGAAACTGAATTGCAGAGAGCAACATATTCAGAGAAGGATGCACTTACCAGAGTTGCGAAGCTTGAGGAGAAAGTGCAACAAAGAGAGAAGGATTTAGAAGCACTTCTGGATAAAGATAAAACACACAGGAAAAATAACGCTGAGATTCTTGAACAACTTTTGGAAACAGAACGTGAAGCACATACACAGGCAAATAACCGGGCCGAGGCTCTCTCTCTGCAGCTACAGTCTGCACAAGCCAAAATTGACTCTCTACATCAAGAGCTGACGAAGTTTCGACTGAATGAAACATTAGACAGTAAGCTAAAAACATCCGATGGTAAGCGATTGAGGGAGGAAAATGACATTGGTGTTGATTCTGTACAAGACATGGATGCGAGCCCTAGAATTTTAAGGGGAACTAAAAGAGCTAGGAGTACATCCAGTCCTAAGTACACTCAGCCAGAAGATGGTGGTTCAATCTTCGAGGGCGGAGAGGATAATCTTAGTCAGCAAACAAATGAAGAGAATTATAAAAAGTTTACCGTCCAGAAGCTTAAGGTAGAACTGACAAAACATAACTATGGTGATCAGCTGCTTCGGTTGAAGAATCCCACCAAGAAAGATATTCTTGCTCTGTATGAGAAGTTTATTCTTCAGAAGGCATAACAGGTTTTGAAAAGACGTGTATTGGTATGCTGCATGTAATCACTCCGTCTAAGTCTCTCTAAATATCATTGATGCCGGGTTTATGAGCTGTGAAATATCGAATTAATTTTAACTCTTTTTGTTGTTGAGTGGTTTTGAGGATGTGTTCTACTTGTGTTTGCTAGCAATGATGATCATTTAGGCCATACAGGGTTTTGATGGCACGATCTTTATTTGTTGATTGTTTGAGTTGTATTTAATACATCTGTACAGTAGCATTTAGCGTATTACTCATAACTCTAACATTGTTTCCATGTTAGATGAACTAAATTACTGAATTAANTAACCT
TARGET=498,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1259
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.095574
PRIMER_LEFT_SEQUENCE=TGAGGAGAAAGTGCAACAAAGA
PRIMER_RIGHT_SEQUENCE=AACACCAATGTCATTTTCCTCC
PRIMER_LEFT=351,22
PRIMER_RIGHT=642,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=0.2291
PRIMER_LEFT_1_PENALTY=0.165541
PRIMER_RIGHT_1_PENALTY=0.063537
PRIMER_LEFT_1_SEQUENCE=CACACAGGAAAAATAACGCTGA
PRIMER_RIGHT_1_SEQUENCE=TCCCCTTAAAATTCTAGGGCTC
PRIMER_LEFT_1=410,22
PRIMER_RIGHT_1=687,22
PRIMER_LEFT_1_TM=60.166
PRIMER_RIGHT_1_TM=59.936
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.2347
PRIMER_LEFT_2_PENALTY=0.171146
PRIMER_RIGHT_2_PENALTY=0.063537
PRIMER_LEFT_2_SEQUENCE=CAGAACGTGAAGCACATACACA
PRIMER_RIGHT_2_SEQUENCE=TCCCCTTAAAATTCTAGGGCTC
PRIMER_LEFT_2=455,22
PRIMER_RIGHT_2=687,22
PRIMER_LEFT_2_TM=59.829
PRIMER_RIGHT_2_TM=59.936
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.2667
PRIMER_LEFT_3_PENALTY=0.171146
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=CAGAACGTGAAGCACATACACA
PRIMER_RIGHT_3_SEQUENCE=AACACCAATGTCATTTTCCTCC
PRIMER_LEFT_3=455,22
PRIMER_RIGHT_3=642,22
PRIMER_LEFT_3_TM=59.829
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=0.3551
PRIMER_LEFT_4_PENALTY=0.059316
PRIMER_RIGHT_4_PENALTY=0.295825
PRIMER_LEFT_4_SEQUENCE=AACTGAATTGCAGAGAGCAACA
PRIMER_RIGHT_4_SEQUENCE=AGTCGAAACTTCGTCAGCTCTT
PRIMER_LEFT_4=291,22
PRIMER_RIGHT_4=569,22
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=59.704
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=279
=
PRIMER_SEQUENCE_ID=SSR_04199 Ps_contig_mira-and-tgicl-ass_franssen_32442
SEQUENCE=CCTTTCAACCCTTCTTACAAGTTTCTCTTTGTCTCTTCATTCATCTTTCATCACCAATATTTCCCTTTATCTTTCTTTCACCTATGCTTCCAATCATTCTTTTGACATGAAACTTCTTCTCTAATAGTATATAACTCTTGTTTCTATTCATCTACGATGCGATTTCCGCGGTTCGGRACCTGTTAACCTAGTGTCTCCATGGCCCGTCCCGATTATCCAACAACATGGATTCCTTACCTAAACAGCAAGGACTGTTCACAAGGCTTTTGTAGCATGTACTGTCCACAATGGTGCTACATAATTTACCCTCCTCCACCACCACCAACGTTTCAATACCCTGATGATGATTCAAAACCGTATTTTTCACCTCTTGTTATTGCTATCATGGGAATTTTAGCCACGGCTTTTCTGTTACTAACTTACTATACTCTAATTTCCAAATACTGTGGCCACAGAGAAACCTCAAGAAGAAATAGCACTGACCCGGTTGAGGAATTCACGCAAAATCGACGCGAAAACTGTCAGGTTTCGACTTTTGGCTTAGATGATGTTCAAATCAAGTCCATTGCAGTTTTCAAATACAAAAAAGGCGACACTTTTTTTGCTGTCACTGATTGTTCCGTTTGTCTAAGTGAGTTTCAAGATGGTGAAAGCGTTAGGCTTTTACCAATGTGTAACCATGTGTTTCATCTTCCTTGTATCGACACATGGCTCAAATCGAACTCTTCTTGTCCTCTATGTCGCGCTAACATATTCACTTTGAATGCTTCAACACTACATGTGTCAGTTCAAGTTCCTGCTACGGTTATTGAATTTCCTTCAAGAAATGAAACTTTTTCGGAAGATGAACAAATAGTTGTGGAAGAAACAAGGATGTATCATCATAGTAGAGTTGATTCGAAGGCGCTTTCGTTGCGCGCTTTTAGTGATCTATGTAATTTGCAAGGTGGAAGAGAAAGAATAGTTGAAATTAGAGATCAAGTGTGTAGGTCAGTTTCTATGGATCATTCACTTCAAAAATGGTTTTTCAATTGGTGATGTGTTGAATATGAATGAAGATGAAGATTTTTGTGAAGAAGGATGTTCAATGGATTCATCAAAGAGATTAAGAGGAGAAAGTAGTAAGTCAAGGTACAAAAGAAAGGTGTTGCATTGTGTTAAGAGTCCAATTGCAATGAAAAGATCATTTTCTAGTGGAACATTTTCACTTGGTAAAATAGATAGAGGAAGAAAGCATGAAATTTTCCATGTTTGAAAATTTATGTTACATTGTTTTTTTTTAATTCTTTTCATGTTATTATTCATATGAGATCATGGATACTATTTTTTTTTGTGTCATAAATGTCNCTAATTTATTAGAAA
TARGET=313,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04200 Ps_contig_mira-and-tgicl-ass_franssen_32444
SEQUENCE=AAAAGAAACAAAAGTAAAGGTAAATACAACGCAGAGTACGCRGGNGATGACAAAGAGAAGGAACCAATCTTAAGAAAAAGCTCTACCCAAAAAAATGAGCTGCTCATTCAGCCTCCATGTTCCCAAAGCTCTACCTCCTCTTCCCTATTATTCATCATCATCACCTTCTCTCTTCATTTCTTCCAAGCTCAAACCATTCCACAACAACACTTCTACTCCTTCCAACCAATCACGACATATAGTAAAATGCAGCACCCAAAAGCCAAAGGATCATGTCAGTCTAAAGAAACCAAATTTGGCACTGCCCATAGGAGCAGTACTCTTGGCCTTGGCTGAGCATCCAGCAGCATTAGCAGTAACTGGAACAAATAATCATCCACAAGAACTCTCATTCATTTTGATACAACTAGGGATTGTGTTCTTTTTCTACTTCATTACTGCACCACCATTGATCTTGTTCTGGCTTTGGAAAAGATGGTACAGAAGAAAGCTTGTTGAAATGTATTTCCAGTTCATGTTTGTCTTCATTTTCTTCCCAGCGTTACTTGTTTGGGTACCGTTTCTAAACTTCAGGAAGTTTCCAAGGGATCCAGACATGGAGTATCCTTGGTCTATACCTGAAGATCCTTCAAAAATAAGAAATGCATACTATAAGTACCCTTTTGCTGATCCTGAGGATTATGACTAGTCATCAAACTACCAATAACAAATCATATGTATCATTTTATGCCTAAACTACACTATTATTGATAAATACGATTTGGTTATACCTTTTTTTTCTGCTTTTGATCTTTGAATGGCCATTCAGCTGTAACTTTGTAAAAATTAAAAGGAACTTTTGAATTCAAAGTTGACACCACATTTCCTANTTTGGTG
TARGET=152,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04201 Ps_contig_mira-and-tgicl-ass_franssen_32447
SEQUENCE=GGTCACTTCTGAGATGACCGGAACCAATCAAATCACGAAGATGAAATCTCATATCGAGTGGAGACATTCGAAGACGCTTCTTTTCCAAAACGGCCAACATCATCTCCTTATATTGACGCCTGCTGAGAAGAGAAATAATTTCCTTCCTTCCCTGGACAAGACCTTTAAGAAGTGAGCCAATATTTGGAATTGCTATCCAATACATGTTTGGATCAATAAGTTGCCTGGTAATGACGCCTGCATTAATTAGAAGGGAGATGTGACTGTCCTTCACCTTTCCCCCAAGTGACAAAAGTGAGCAAAGCTCCTGATGCTCAATGCCAGTTTCCAGCTTCGAATCTAGGACATGAATTTTGAACCACTCAAAAACTTCAAATTCTGCTCGTGTCTTTCCTTCCATTCTTTTAAAAACACGATCTATCTGTTTTAGGTAATCATCCAAAAACATTACAGCATGATCATCTTGTCCAGTATTTAATTTAAAAAGGCGAAGCACTTTATCCCTCCGAAGAGACTCTAATTCCCTATCCACTTGTGTTCTGTTCTTCACACTGCTATACAATTGAGACTTCAATATAAACGGTTCAACCGAAACATTGAGAATTTGGGGAAATTGAGCTCGCATGATTCGAAGCGCAATAAGCGTATC
TARGET=140,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1625
PRIMER_LEFT_PENALTY=0.101680
PRIMER_RIGHT_PENALTY=0.060824
PRIMER_LEFT_SEQUENCE=ACATTCGAAGACGCTTCTTTTC
PRIMER_RIGHT_SEQUENCE=AGGAGCTTTGCTCACTTTTGTC
PRIMER_LEFT=63,22
PRIMER_RIGHT=308,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.1862
PRIMER_LEFT_1_PENALTY=0.125411
PRIMER_RIGHT_1_PENALTY=0.060824
PRIMER_LEFT_1_SEQUENCE=TTATATTGACGCCTGCTGAGAA
PRIMER_RIGHT_1_SEQUENCE=AGGAGCTTTGCTCACTTTTGTC
PRIMER_LEFT_1=107,22
PRIMER_RIGHT_1=308,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=60.061
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.2167
PRIMER_LEFT_2_PENALTY=0.101680
PRIMER_RIGHT_2_PENALTY=0.115007
PRIMER_LEFT_2_SEQUENCE=ACATTCGAAGACGCTTCTTTTC
PRIMER_RIGHT_2_SEQUENCE=CCTTCTAATTAATGCAGGCGTC
PRIMER_LEFT_2=63,22
PRIMER_RIGHT_2=253,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.115
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.2336
PRIMER_LEFT_3_PENALTY=0.125411
PRIMER_RIGHT_3_PENALTY=0.108206
PRIMER_LEFT_3_SEQUENCE=TTATATTGACGCCTGCTGAGAA
PRIMER_RIGHT_3_SEQUENCE=GAAGGAAAGACACGAGCAGAAT
PRIMER_LEFT_3=107,22
PRIMER_RIGHT_3=396,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.2349
PRIMER_LEFT_4_PENALTY=0.125411
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=TTATATTGACGCCTGCTGAGAA
PRIMER_RIGHT_4_SEQUENCE=AAGAATGGAAGGAAAGACACGA
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=403,22
PRIMER_LEFT_4_TM=59.875
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_04202 Ps_contig_mira-and-tgicl-ass_franssen_32454
SEQUENCE=CCGGTTTGTGGAGAAGCAACCCAATCGCGAAGTGGGCGCGAGATAGATAGAGAAAATGGTGGAAGCTCTGAAACTGTTGAACCTAACATGGCACCCACTAAAGAACCATTTCCTTCACTTCTCCACTTACGCATTTCGTCGAACACCTCGCACCCTTCTTATCCGCGCGTCCTCGTCTACAACTGCCGCCTCGGGTCGCAACCGCCGGAGCTCTGCATCGTCCCCCACCACTTCCACCTCCGATAGGGAGGCCATTCGAGCCATCCGTTTGAAAAAGGTTGAAGAAATTAAAAGCAAGGGACTCAATCCCTATGCTTATGAATGGAACAAAACACACAGTGCAAAGCAGTTGCAAGATATATATAAAGATTTAGCAAATGGTGAGGAAACTAATAGTGAGAATGATCATGTTTCTATTGCGGGAAGAATTATCGCAAGGAGGGCATTTGGGAAGCTTGCCTTTTTGACACTAAGGGATGACTCAGGAACAATTCAGCTCTATTGCGAAAAGGAGAGACTTGTAGATGAACAGTTTGAGCAATTGAAGGCACATGTTGATATTGGTGATATAGTAGGTGCAAAAGGAACAATAAAACGCACAGAAAAAGGAGAACTTTCTGTGTGTGTGCTATCTTTTGCAATTCTTACAAAATCTTTGCTTCCACTGCCTGACAAATATCATGGTCTAACTGATATAGATAAACGCTATCGGCAGAGGTATGTAGATATGATTGCAAATCCAGAGGTAGCTGATGTATTTCGCAAAAGAGCAAAGGTTGTATCAGGGATACGAAGGACAATGGATTCTTTAGGTTATGTTGAGGTAGAAACTCCAGTTTTGCAGGGAGCAGCTGGGGGAGCAGAGGCCAGACCATTTGTTACATATCATAATTCTCTTGGAAGGGACTTATATTTGAGAATTGCAACTGAATTGCACCTAAAGAGAATGCTGGTGGGTGGGTTTGAAAAAGTATATGAAATTGGTCGAATATTTAGAAATGAAGGAATTTCAACTCGTCATAATCCTGAGTTTACAACTATAGAGATGTATGAAGCATACTCAGACTACCAAAGCATGATGAATTTGGCAGAGGAAATTGTCACTCAATGTGCTCTGGCAGTTCACGGGAAGCTTACTATTGATTATCAGGGCGTGGAGATATGTCTGGAAAAGCCTTGGAGGAGAGAGACCATGCACAATCTTGTTAAAGAAGTTTCTGGGATCGATTTCAATGAATTGGGAAATGATCTTGAGGTTGCAAAACAAGCTACTTTGGCTAGCCTTGAAAATAATCTTGATAACAAGGATAAAGCTTCCATTGAAGCGTGCCAATCTGTTGGCCACCTCCTTAACGAGGTTTTTGAATTATTTGTAGAACCGAAGCTTATCCAACCTACATTTGTTTTAGACTACCCAATTGAGATATCTCCACTGGCTAAACCACACCGCAGGTCCACAGGTTTGACTGAGAGGTTTGAACTTTTCATTTGTGGTCGTGAACTAGGCAATGCATTCTCGGAATTGACCGATCCTATAGATCAGAGAGGACGTCTAGAAGATCAAGTTAAACAGCACGAAAAGAAAAGAGCCGCCGTTTCAGCAAATGGAGATAAGAAAGAAGGAATAGAAAATGATGATGATCATTCATATGAAGTGACTCTTGATGAAGATTTCCTTACAGCTTTGGAGTATGGAATGCCTCCAGCTTCTGGAATGGGACTTGGAATCGATAGGCTCGTGATGCTTCTGACAAATTCTCCCAGTATTAGAGATGTAATACCCTTCCCAGTACTCAAGGTCCAACAATAGACAGTTTTGTTATTTTAGCAGTTAATGTAATAACAGAATAAAAAGTCGTCAGTTATTTTCTCTTTCTAATGTCATTGTTAAGCCAGTTTAATTATTATTAGTAGTATGATTTTTATTATTATTAATTACATTTGAGTTCTGTGTATATCACATGGAATTAGATGTGTAACTGAGATAGTTTTCCATCTTTGACTGAATTGGAAAATGATGAGATGTGTAANAANTATAGTTTGAGATTATTTTATTGTTTGTTATAGAA
TARGET=619,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1353
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.134602
PRIMER_LEFT_SEQUENCE=GCCTTTTTGACACTAAGGGATG
PRIMER_RIGHT_SEQUENCE=TACCTCTGCCGATAGCGTTTAT
PRIMER_LEFT=457,22
PRIMER_RIGHT=719,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.135
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.1353
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.134602
PRIMER_LEFT_1_SEQUENCE=GCCTTTTTGACACTAAGGGATG
PRIMER_RIGHT_1_SEQUENCE=ATACCTCTGCCGATAGCGTTTA
PRIMER_LEFT_1=457,22
PRIMER_RIGHT_1=720,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.135
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.1353
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.134602
PRIMER_LEFT_2_SEQUENCE=TGCCTTTTTGACACTAAGGGAT
PRIMER_RIGHT_2_SEQUENCE=TACCTCTGCCGATAGCGTTTAT
PRIMER_LEFT_2=456,22
PRIMER_RIGHT_2=719,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.135
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.3000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=264
PRIMER_PAIR_PENALTY_3=0.1353
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.134602
PRIMER_LEFT_3_SEQUENCE=TGCCTTTTTGACACTAAGGGAT
PRIMER_RIGHT_3_SEQUENCE=ATACCTCTGCCGATAGCGTTTA
PRIMER_LEFT_3=456,22
PRIMER_RIGHT_3=720,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.135
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=265
PRIMER_PAIR_PENALTY_4=0.2029
PRIMER_LEFT_4_PENALTY=0.072552
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=CGCACAGAAAAAGGAGAACTTT
PRIMER_RIGHT_4_SEQUENCE=TCCCTGATACAACCTTTGCTCT
PRIMER_LEFT_4=595,22
PRIMER_RIGHT_4=787,22
PRIMER_LEFT_4_TM=59.927
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_04203 Ps_contig_mira-and-tgicl-ass_franssen_32454
SEQUENCE=CCGGTTTGTGGAGAAGCAACCCAATCGCGAAGTGGGCGCGAGATAGATAGAGAAAATGGTGGAAGCTCTGAAACTGTTGAACCTAACATGGCACCCACTAAAGAACCATTTCCTTCACTTCTCCACTTACGCATTTCGTCGAACACCTCGCACCCTTCTTATCCGCGCGTCCTCGTCTACAACTGCCGCCTCGGGTCGCAACCGCCGGAGCTCTGCATCGTCCCCCACCACTTCCACCTCCGATAGGGAGGCCATTCGAGCCATCCGTTTGAAAAAGGTTGAAGAAATTAAAAGCAAGGGACTCAATCCCTATGCTTATGAATGGAACAAAACACACAGTGCAAAGCAGTTGCAAGATATATATAAAGATTTAGCAAATGGTGAGGAAACTAATAGTGAGAATGATCATGTTTCTATTGCGGGAAGAATTATCGCAAGGAGGGCATTTGGGAAGCTTGCCTTTTTGACACTAAGGGATGACTCAGGAACAATTCAGCTCTATTGCGAAAAGGAGAGACTTGTAGATGAACAGTTTGAGCAATTGAAGGCACATGTTGATATTGGTGATATAGTAGGTGCAAAAGGAACAATAAAACGCACAGAAAAAGGAGAACTTTCTGTGTGTGTGCTATCTTTTGCAATTCTTACAAAATCTTTGCTTCCACTGCCTGACAAATATCATGGTCTAACTGATATAGATAAACGCTATCGGCAGAGGTATGTAGATATGATTGCAAATCCAGAGGTAGCTGATGTATTTCGCAAAAGAGCAAAGGTTGTATCAGGGATACGAAGGACAATGGATTCTTTAGGTTATGTTGAGGTAGAAACTCCAGTTTTGCAGGGAGCAGCTGGGGGAGCAGAGGCCAGACCATTTGTTACATATCATAATTCTCTTGGAAGGGACTTATATTTGAGAATTGCAACTGAATTGCACCTAAAGAGAATGCTGGTGGGTGGGTTTGAAAAAGTATATGAAATTGGTCGAATATTTAGAAATGAAGGAATTTCAACTCGTCATAATCCTGAGTTTACAACTATAGAGATGTATGAAGCATACTCAGACTACCAAAGCATGATGAATTTGGCAGAGGAAATTGTCACTCAATGTGCTCTGGCAGTTCACGGGAAGCTTACTATTGATTATCAGGGCGTGGAGATATGTCTGGAAAAGCCTTGGAGGAGAGAGACCATGCACAATCTTGTTAAAGAAGTTTCTGGGATCGATTTCAATGAATTGGGAAATGATCTTGAGGTTGCAAAACAAGCTACTTTGGCTAGCCTTGAAAATAATCTTGATAACAAGGATAAAGCTTCCATTGAAGCGTGCCAATCTGTTGGCCACCTCCTTAACGAGGTTTTTGAATTATTTGTAGAACCGAAGCTTATCCAACCTACATTTGTTTTAGACTACCCAATTGAGATATCTCCACTGGCTAAACCACACCGCAGGTCCACAGGTTTGACTGAGAGGTTTGAACTTTTCATTTGTGGTCGTGAACTAGGCAATGCATTCTCGGAATTGACCGATCCTATAGATCAGAGAGGACGTCTAGAAGATCAAGTTAAACAGCACGAAAAGAAAAGAGCCGCCGTTTCAGCAAATGGAGATAAGAAAGAAGGAATAGAAAATGATGATGATCATTCATATGAAGTGACTCTTGATGAAGATTTCCTTACAGCTTTGGAGTATGGAATGCCTCCAGCTTCTGGAATGGGACTTGGAATCGATAGGCTCGTGATGCTTCTGACAAATTCTCCCAGTATTAGAGATGTAATACCCTTCCCAGTACTCAAGGTCCAACAATAGACAGTTTTGTTATTTTAGCAGTTAATGTAATAACAGAATAAAAAGTCGTCAGTTATTTTCTCTTTCTAATGTCATTGTTAAGCCAGTTTAATTATTATTAGTAGTATGATTTTTATTATTATTAATTACATTTGAGTTCTGTGTATATCACATGGAATTAGATGTGTAACTGAGATAGTTTTCCATCTTTGACTGAATTGGAAAATGATGAGATGTGTAANAANTATAGTTTGAGATTATTTTATTGTTTGTTATAGAA
TARGET=1923,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04204 Ps_contig_mira-and-tgicl-ass_franssen_32466
SEQUENCE=AAGCAGGTGGTATCAACGCAGATACGCGGGGCCTCAGTAGTTGAAATATCTCTCTCTCTCTCTCTCTCTGAGCAAGTTGTTTTCTTTAATCTTTCATATCCCAAATCTAATCTGTATCTAGCTTCTACCATTCTATCCAAAAAAAATCAGAGTGAACTAACATAATTGTGAAACTAGAAAATATTTAAAGGAACTGATATGAAGTGAGATATTTTTCAAACATGGAGGAAGGAATCAAAGATGGATCAAAAAGAAAAAACATCCTTAGTTCTTACAAAGGCTTCTCAAGAATACAACACTTTCCTTCCAAATCAAAGAACTGTGCTGTTGGTAATGTTTCTGAAAATGCTGCTGATAGGTTTAAAAAAGCAGAAGAATCACTTAGAACTGTCATGTACTTGAGTTGTTGGGGTCCTAATTAAATTAAAGGTGAAGGTTAAATTATGATAGCTACTTTGTCTTGTTTGTTTTAGTAGTGGTTTTTGAGAATATATATTATGTAGTAGCTTAATGTTCATAGAGGTTTGTAAATTCTTCATGTTAGTAGAACATAATAAACTAACTCATATTTTCCTTTTTCTGTGAGTGTTGGAATGGTTATAATATTATTAGTTTTTTTTGAACGACG
TARGET=49,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.3155
PRIMER_LEFT_PENALTY=3.255655
PRIMER_RIGHT_PENALTY=0.059875
PRIMER_LEFT_SEQUENCE=AGCAGGTGGTATCAACGCAG
PRIMER_RIGHT_SEQUENCE=TCTTTGATTCCTTCCTCCATGT
PRIMER_LEFT=1,20
PRIMER_RIGHT=240,22
PRIMER_LEFT_TM=61.256
PRIMER_RIGHT_TM=59.940
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=3.5522
PRIMER_LEFT_1_PENALTY=3.492277
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=AAGCAGGTGGTATCAACGCAG
PRIMER_RIGHT_1_SEQUENCE=TCTTTGATTCCTTCCTCCATGT
PRIMER_LEFT_1=0,21
PRIMER_RIGHT_1=240,22
PRIMER_LEFT_1_TM=62.492
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=3.6238
PRIMER_LEFT_2_PENALTY=3.563903
PRIMER_RIGHT_2_PENALTY=0.059875
PRIMER_LEFT_2_SEQUENCE=CGGGGCCTCAGTAGTTGAAAT
PRIMER_RIGHT_2_SEQUENCE=TCTTTGATTCCTTCCTCCATGT
PRIMER_LEFT_2=26,21
PRIMER_RIGHT_2=240,22
PRIMER_LEFT_2_TM=62.564
PRIMER_RIGHT_2_TM=59.940
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=3.6317
PRIMER_LEFT_3_PENALTY=3.571781
PRIMER_RIGHT_3_PENALTY=0.059875
PRIMER_LEFT_3_SEQUENCE=GTATCAACGCAGATACGCGG
PRIMER_RIGHT_3_SEQUENCE=TCTTTGATTCCTTCCTCCATGT
PRIMER_LEFT_3=9,20
PRIMER_RIGHT_3=240,22
PRIMER_LEFT_3_TM=61.572
PRIMER_RIGHT_3_TM=59.940
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=13.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=232
PRIMER_PAIR_PENALTY_4=3.7957
PRIMER_LEFT_4_PENALTY=3.255655
PRIMER_RIGHT_4_PENALTY=0.540037
PRIMER_LEFT_4_SEQUENCE=AGCAGGTGGTATCAACGCAG
PRIMER_RIGHT_4_SEQUENCE=CTTTGATTCCTTCCTCCATGTT
PRIMER_LEFT_4=1,20
PRIMER_RIGHT_4=239,22
PRIMER_LEFT_4_TM=61.256
PRIMER_RIGHT_4_TM=59.460
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_04205 Ps_contig_mira-and-tgicl-ass_franssen_32471
SEQUENCE=GGTTTNCTTTAAAATTAGAGGTGTTTTCTCTATTATCCAAGTTTCATAGAACTTTGGTATACATCAGCCATAGTAAGCTGGCATTTCATCAAGTTACAAAACAAAAAAAGAAAAGAAAAGCAAGACTGTGTTTACATACACTTAGTTTAAGCTTAAGAGGACACTCATTAAAAAGAAGGAGTGTCCTTAGCTTAACCAAACTAGAGCCCCATCACATATACTTCTATGTATACATGGATCTAGCTCTTTGAAGTTTTATTCATAAGTTATTACTAGAACCAAATGGCTCTAGCAGCCATGAGCATTTGCTTGAGAGAACCATCAACATGGAGAGAAATCACATCCTTTGATGACCCTACAAATTTTCCACCAATGAAAACTGCTGGAACTGAAGGGTTACATCCAAGGCTCCTTAGAGCCCTTTCCATTTTCCCCTACCATAGGGTTCAATGTCAAGGTCATGCACTGCAGGGCTTGCTCCCAGCTCATAGAAGAGCTGTGTTATGCTGTGGCACATGTAGCATGAGCTCTTTGTGAATACAACTGCAGCTTTCTTTGATGCCAAATCCTTGACTCTATCCATTACTTGAAAT
TARGET=106,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2031
PRIMER_LEFT_PENALTY=0.142943
PRIMER_RIGHT_PENALTY=0.060196
PRIMER_LEFT_SEQUENCE=ATCAGCCATAGTAAGCTGGCAT
PRIMER_RIGHT_SEQUENCE=GGGTCATCAAAGGATGTGATTT
PRIMER_LEFT=62,22
PRIMER_RIGHT=355,22
PRIMER_LEFT_TM=60.143
PRIMER_RIGHT_TM=60.060
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=0.2540
PRIMER_LEFT_1_PENALTY=0.142943
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=ATCAGCCATAGTAAGCTGGCAT
PRIMER_RIGHT_1_SEQUENCE=CTCCATGTTGATGGTTCTCTCA
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=331,22
PRIMER_LEFT_1_TM=60.143
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.2764
PRIMER_LEFT_2_PENALTY=0.216250
PRIMER_RIGHT_2_PENALTY=0.060196
PRIMER_LEFT_2_SEQUENCE=AGCCATAGTAAGCTGGCATTTC
PRIMER_RIGHT_2_SEQUENCE=GGGTCATCAAAGGATGTGATTT
PRIMER_LEFT_2=65,22
PRIMER_RIGHT_2=355,22
PRIMER_LEFT_2_TM=59.784
PRIMER_RIGHT_2_TM=60.060
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.2902
PRIMER_LEFT_3_PENALTY=0.142943
PRIMER_RIGHT_3_PENALTY=0.147295
PRIMER_LEFT_3_SEQUENCE=ATCAGCCATAGTAAGCTGGCAT
PRIMER_RIGHT_3_SEQUENCE=TGTAGGGTCATCAAAGGATGTG
PRIMER_LEFT_3=62,22
PRIMER_RIGHT_3=359,22
PRIMER_LEFT_3_TM=60.143
PRIMER_RIGHT_3_TM=59.853
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.3273
PRIMER_LEFT_4_PENALTY=0.216250
PRIMER_RIGHT_4_PENALTY=0.111077
PRIMER_LEFT_4_SEQUENCE=AGCCATAGTAAGCTGGCATTTC
PRIMER_RIGHT_4_SEQUENCE=CTCCATGTTGATGGTTCTCTCA
PRIMER_LEFT_4=65,22
PRIMER_RIGHT_4=331,22
PRIMER_LEFT_4_TM=59.784
PRIMER_RIGHT_4_TM=60.111
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_04206 Ps_contig_mira-and-tgicl-ass_franssen_32473
SEQUENCE=TATCTCTCTTATATCTGGYTYTNCNACTTCTCTCYYCCTCTCTCTCTCTCAAACTTTTGAATTCATCAAAGATCTTATCTTTTTGATAAAACCACAAAACCCTAGATCGCAATTGATCTAAACTTGGAAACTTCATGGACACGAAGCTCCTGAATTTCCAGATCCTTAATGGGTCAATCATTCGGCGCGTGTTCTTCCGCTCAATCATGCTTGCAGCTACCATTTCCATGGTGGCTCTCTTTCGCGCTTTATCAGATTTCGATTTGGGAAATTTGGTTCCCATTGCAAACAGTTTCAGCTGTTTTGCAGATTCGGATGACAACGTTACATCCGAACCCCAGTTCAAATTTCTTTCAGAATCGAATCTGGGGCAACATGAATTGTGAAAAAGACGTTAACTTGACAGTGAATGTCGTCACTGAGTTAATGGGTAGACAGTTTTTGAACTGTGAAGCGAATACCCTTTGTGTTGGTGAAGGTTCTTCAATGGCCGTTAAGGCAATGAAACAGTTGGGTTTTTCAACTGTTAGTGGTGTTTACACTAACCGTTTTTTCACACTCAATATGAAGAGAATCGTTTATGAGCTGGATTATCAAGATTCTTCATTTGATTTTGTTTTGTCTAGAGATCTTGATAAGGTTTCTGTTCCTGCTTTGCTTGTACTTGAGGTTGAACGTGTTCTTAAGCCTAACGGAATTGGTGCACTTCTTGTTGGTGCTAAAAGTTCACACCATAATGATTTGATTAGATCAGCTACACCAGTTTCATCATTGTTGAGATCTTCCAGTGTGGTTCATGTTGATTCTATTGATGATGAACTTAACCTTGTTGTTTTCAAGAAAAGATCTGAAAATGCAACAGCTTTTTTCAACCACCATCAGCTTAGTTTACCTACTGATTGTCCTAGTTTGGCGTTAACCAAACCTCTTATAGATCTGATGGAACCTCTTGTGAGTGAGAAACCGAAACCGTCTAACGCGACGCCGGTTTCATATTTGCCCAAGTTTGTAGATGTTTCTACTAGGAAGAGGTTGGTGTATATTGATATTGGGGTAGGAGAGCTTTTGAATGCAAATGTTAGTGATTGGTTTCTACCTTCATATCCAATTGATCAGAAAGATTTCAATGTTTATTTTGTTCACTACAACACTTCCATAATGTTGTCTTATGTGAAGAGACCTGGTATCACGTTTGTTTATCACCCTGGATTAGCCGGTAAGGTCGTCACCGAGGCCGATGTTGATGTTGACGAGGAGGAAGATGAAGAAGTGGATCCTTATGCTGGGGAGGAAGAGTTTGATTTCCTTGCATGGTTCAAAGAAACAGTGCAATATGCTGATTTTGTTGTGTTGAAGATGAATGCAGGTGAAGTTGAACTCAAGTTTCTTTCTGATGTATTTGACAGTGGAGCTATATGCTTTGTTGATGAGTTGTTTCTCAGATGTCCTGATATCACAAGCAAGGAAAGTTGCATGGATGTTTACAAAAGTCTGAGAAGCAATGGTGTCTATGTGCATCAATTGTGGGGAGACTAAATTCCATTAGCCTTTGGTGACAATATGTGTGGAAAAAAATATGTTGTGTTATTTGAATTTGTGTGTTGTTGTTGTTGTTGTTGTATGTTAAGGTTACTACGTAAGATGCTATGTCATCTGAGTGCTATATGGTTGGGTGCTTCTACTAAATAAACAACCATTGTTGTGTACTTCTGGTTTGCTAGTAAATCATGTGTGGATTGTTAAGT
TARGET=38,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04207 Ps_contig_mira-and-tgicl-ass_franssen_32473
SEQUENCE=TATCTCTCTTATATCTGGYTYTNCNACTTCTCTCYYCCTCTCTCTCTCTCAAACTTTTGAATTCATCAAAGATCTTATCTTTTTGATAAAACCACAAAACCCTAGATCGCAATTGATCTAAACTTGGAAACTTCATGGACACGAAGCTCCTGAATTTCCAGATCCTTAATGGGTCAATCATTCGGCGCGTGTTCTTCCGCTCAATCATGCTTGCAGCTACCATTTCCATGGTGGCTCTCTTTCGCGCTTTATCAGATTTCGATTTGGGAAATTTGGTTCCCATTGCAAACAGTTTCAGCTGTTTTGCAGATTCGGATGACAACGTTACATCCGAACCCCAGTTCAAATTTCTTTCAGAATCGAATCTGGGGCAACATGAATTGTGAAAAAGACGTTAACTTGACAGTGAATGTCGTCACTGAGTTAATGGGTAGACAGTTTTTGAACTGTGAAGCGAATACCCTTTGTGTTGGTGAAGGTTCTTCAATGGCCGTTAAGGCAATGAAACAGTTGGGTTTTTCAACTGTTAGTGGTGTTTACACTAACCGTTTTTTCACACTCAATATGAAGAGAATCGTTTATGAGCTGGATTATCAAGATTCTTCATTTGATTTTGTTTTGTCTAGAGATCTTGATAAGGTTTCTGTTCCTGCTTTGCTTGTACTTGAGGTTGAACGTGTTCTTAAGCCTAACGGAATTGGTGCACTTCTTGTTGGTGCTAAAAGTTCACACCATAATGATTTGATTAGATCAGCTACACCAGTTTCATCATTGTTGAGATCTTCCAGTGTGGTTCATGTTGATTCTATTGATGATGAACTTAACCTTGTTGTTTTCAAGAAAAGATCTGAAAATGCAACAGCTTTTTTCAACCACCATCAGCTTAGTTTACCTACTGATTGTCCTAGTTTGGCGTTAACCAAACCTCTTATAGATCTGATGGAACCTCTTGTGAGTGAGAAACCGAAACCGTCTAACGCGACGCCGGTTTCATATTTGCCCAAGTTTGTAGATGTTTCTACTAGGAAGAGGTTGGTGTATATTGATATTGGGGTAGGAGAGCTTTTGAATGCAAATGTTAGTGATTGGTTTCTACCTTCATATCCAATTGATCAGAAAGATTTCAATGTTTATTTTGTTCACTACAACACTTCCATAATGTTGTCTTATGTGAAGAGACCTGGTATCACGTTTGTTTATCACCCTGGATTAGCCGGTAAGGTCGTCACCGAGGCCGATGTTGATGTTGACGAGGAGGAAGATGAAGAAGTGGATCCTTATGCTGGGGAGGAAGAGTTTGATTTCCTTGCATGGTTCAAAGAAACAGTGCAATATGCTGATTTTGTTGTGTTGAAGATGAATGCAGGTGAAGTTGAACTCAAGTTTCTTTCTGATGTATTTGACAGTGGAGCTATATGCTTTGTTGATGAGTTGTTTCTCAGATGTCCTGATATCACAAGCAAGGAAAGTTGCATGGATGTTTACAAAAGTCTGAGAAGCAATGGTGTCTATGTGCATCAATTGTGGGGAGACTAAATTCCATTAGCCTTTGGTGACAATATGTGTGGAAAAAAATATGTTGTGTTATTTGAATTTGTGTGTTGTTGTTGTTGTTGTTGTATGTTAAGGTTACTACGTAAGATGCTATGTCATCTGAGTGCTATATGGTTGGGTGCTTCTACTAAATAAACAACCATTGTTGTGTACTTCTGGTTTGCTAGTAAATCATGTGTGGATTGTTAAGT
TARGET=1600,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04208 Ps_contig_mira-and-tgicl-ass_franssen_32476
SEQUENCE=AGTGGGGGACATCATTTTCTGATGCCCGAAAAAAAGAAGAGAAACATAAGATATGGATGAATGGATCAACCGCTTCTTTTCTTCTTTCTTCTTCTTCCATTCCAATTTCTCTAACCCATTTCTCTAAACTTCACCTTTTTCTCTGAATTCATCAATTTGTTGAAGAAATGGCTATGCCTATCACTGCTTCCAGTCTTGGATTTCACTTCACTGCTGCTACTCAAAACCCTATTTTCTGTGTTCCTTTTTTGAGGGCTTCTTCTGCGTTTCATGCTACTAAGCCATGTTACTTTCTTCACAAAACTAGATTGTTATCCTCTGGAACTGCGATTATCACTCGAGCAGCAGCACCTGTTACTAATGTGCAGGATGGTAATCAAGGTGAGACTGATACCGTTCCGATCCCGATAGTCATAATTGACCAAGATTCTGATCCAAATGCAACTGTGGTGAAGATAACTTTTGGTGATCGGCTTGGAGCTCTCCTTGACACAATGAGTGCATTAAAAAGCTTAGGCCTCAATGTTGTTAAGGCAAATGTCTTTCTGGATTCTTCTGGCAAACACAACAAGTTTTCCATCACAAAAGCTGATACTGGCAGAAAAGTCGAAGATCCAGAGTTGTTAGAAGCAATTCGTTTGACAATTATAAATAATCTGATTCAGTATCACCCGGAATCAAGTTCTCAGTTAGCAATGGGAGCAGCTTTTGGACTTCTACCTCCAAAAGAGCAGGTTGATGTGGACATTGCAACTCGCATAAATATTTCTGATGATGGTCCAGATCGAAGTTTGTTCTATGTGGAGACAGCTGATCACCCTGGATTACTCGTCGAGCTTGTAAAAAGTATTACTGACATTGACATTGCTGTTGAATCTGGAGAATTCGATACTGAGGGGCTCTTGGCTAAGGCAAAGTTTCATGTTAGCTACAAAGGCAAAGCAATCGGCAAGCAGCTGCAGCAGGTTCTTGCTAACAGCTTGAGATATTTCCTGAGGCGTCCGACAACGGAGGAATCCAGTTTTTGAAGGGCATCTCTTTCAATTTAAATAAAGTACATGACATTATATATTCTTTTGAAGATTAACCACCATCATGGATTGGTGAATGCTTGCATTCATTGTTTCATTTGGAAGAGAAATGAAGAGTAACTGGAATGCATTTACATCATTAAACTAAATTAACAAGGATATGATTGTAAAGTTACTTTTGCTAATTTCTTACATAAAATTATTTGCAACTTCAAATGCTTTTGATATTTGGGCAGAGTATTCATTTCATATACAGTATTCATGGTAAGGATCATGAATAAAGGTGTTGGAACATGTCTAACTTCCAAAATGGGGATCAATTTGAAAGAAATTTTAAGTCCCACGTTGTTAGAAAACTATGTGCCTCAATATGTTAATTGGTGTGTTAATCGAGAAAACTCGGACCCACGTGCTTACTTACACATTATTAGTTAGTTTCAGTGGTTAAGTCTTATTTCATTATTAATAAAAAAATACACAATTAGTCTTATTTCTAAA
TARGET=86,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8594
PRIMER_LEFT_PENALTY=0.773040
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=ATATGGATGAATGGATCAACCG
PRIMER_RIGHT_SEQUENCE=CAGCAGTGAAGTGAAATCCAAG
PRIMER_LEFT=50,22
PRIMER_RIGHT=215,22
PRIMER_LEFT_TM=60.773
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.8597
PRIMER_LEFT_1_PENALTY=0.773040
PRIMER_RIGHT_1_PENALTY=0.086698
PRIMER_LEFT_1_SEQUENCE=ATATGGATGAATGGATCAACCG
PRIMER_RIGHT_1_SEQUENCE=AAGACTGGAAGCAGTGATAGGC
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=196,22
PRIMER_LEFT_1_TM=60.773
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.8977
PRIMER_LEFT_2_PENALTY=0.773040
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=ATATGGATGAATGGATCAACCG
PRIMER_RIGHT_2_SEQUENCE=TCCAAGACTGGAAGCAGTGATA
PRIMER_LEFT_2=50,22
PRIMER_RIGHT_2=199,22
PRIMER_LEFT_2_TM=60.773
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=0.9121
PRIMER_LEFT_3_PENALTY=0.773040
PRIMER_RIGHT_3_PENALTY=0.139083
PRIMER_LEFT_3_SEQUENCE=ATATGGATGAATGGATCAACCG
PRIMER_RIGHT_3_SEQUENCE=ATCCAAGACTGGAAGCAGTGAT
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=200,22
PRIMER_LEFT_3_TM=60.773
PRIMER_RIGHT_3_TM=60.139
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.9160
PRIMER_LEFT_4_PENALTY=0.773040
PRIMER_RIGHT_4_PENALTY=0.142943
PRIMER_LEFT_4_SEQUENCE=ATATGGATGAATGGATCAACCG
PRIMER_RIGHT_4_SEQUENCE=AGCAGTGATAGGCATAGCCATT
PRIMER_LEFT_4=50,22
PRIMER_RIGHT_4=187,22
PRIMER_LEFT_4_TM=60.773
PRIMER_RIGHT_4_TM=60.143
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_04209 Ps_contig_mira-and-tgicl-ass_franssen_32482
SEQUENCE=CGGTAGCCAGGTCGGGTTCATGTTCATTCCCCGTCCGGTCAGATCGACGCCGGGAGAACACAGTACATAGCCGCCACGTTCTCTGTCCAGTCTTTCCCGTTGTCGGCACCGCCGAATATGGGGCCCACACTGGCGAACGGTGATGGTGTAGGGTTTTAACTTCGGGTTGAGAGTTCAACCGACGATGGAGATTGAGTCGAAGCTGGAAATGGCAGGGCGTGTTAGGGTTTTGCATTTCTTCAGTCATGAAGTGAAGGTTAGAACCGGTTGCAGAGTCGCCATTGCCGTTAGTGACGGTTCTGTTTTAGGTTTCCATTGCTAAGTTTTCTTAGTGGTAACTCTAAATATTATATATATCAAAATTAATTGTTAGTGATGTTTAGTAAGGAAATCATGGGAAAACAAAATTGTAAAAATGGACTTTTCAGATTATAAATTTTGAATGCAGATTTTGACATTGTTTGTTTGTTTTTGATATTATACCATTAGGAATCTCTTGCC
TARGET=458,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04210 Ps_contig_mira-and-tgicl-ass_franssen_32486
SEQUENCE=GTCGRGATAACAAGTGATTTATTAAGCTCTGAATTGGGAGTGGAAGAGTCTGTGTGTGTGTTGGGGTTCTTTCTTCGCTTCCTTCCTATGGATCTTCATGCCGACTCTCCCAATTCCGGGATAAAGAAACGAAAACAAGACGAACAGGATGCTTTGGTCAGTGATCATGGAAATTCCATGAGTGTTGGAGAATCATCTGGTAGTGGTGACCCCTGCAAGAGTTTGACCTCTATATCATTGAGTTCAAAGAATTCAAATGGTTCCGATTCTGATATTTCATGTCATGACGATGATGAGGATGTGATTGATGATGATGATGACGCACTTGAAGATCCTGATTATGCATCTGATTTTTATGACGATGATGACGTCTATGAAGATGACTATTCACTTATGCAGGATCAATTTGACAATGTGGATTTACCTACTGGGGTAGAAGCATCACTACCGTGGTTGAAGGATATTGCTTCTAGCGAATGCAAGCCGGCTGTTGTTCATGCCCAGTCTGAGTCCAGTTCGAAAGAAAAAGTAGATGAGGCCACGGATGCCGTCATGCAAAAGTTTCGTCAATTTAAGCCATTTGATACTGTTGATTCTTTTCCTGATCATCACTATGATAAGGAAGGTGCTTCAGTCTTAGAGGCGCAGCGACCAAAGAATTGGGCCAAAAAAATTCAGGAGGAATGGAAAATTTTGGAGGAAAATTTGCCAGAAACAATCTTTGTGAGAGTTTGTGAATCTAAAATGGAGCTTCTTAGGGCTGTCATCATTGGACCTCAAGGCACTCCATACCATGATGGCCTATTCTTTTTCGATTGTTTATTTCCTTCTTCATACCCCGCAGGTCCACCGCAAGTTCACTATCACTCAGGTGGCCTAAGGCTAAATCCAAATCTGTATCAATGTGGAAAAGTGTGCCTTAGTCTCTTGGGTACTTGGCATGGTAAGAATAGCGAAAACTGGATTCCAGATAAATCAACCATGCTACAAGTCTTGGTTTCTATACAAGCTCTTATTCTGAATGAGAAACCTTTTTTTAATGAGCCTGGATATTCTGATGCATATCCTAGTGAGGAGGGGCAAAGGAGATCTAAGGATTACAATGATAATACATTTATTTTATCTTTGAAGACAATGATGTATACCCTGCGTAAACCGCCAAAGCACTTTGAGGACTTCGTTGCGGGGCATTTCCGTCAAAGGGGCGTGGACATACTGAAAGCATGTAGGTCATATGCTGAGGGGGGACCAGTTGGATCAGTTTTACATAATACTCGTAGTATTAATGCTTCTGCCAAGAACCAAAAAGAATTTCAGTCAACTGTGAGTAGGATGATGAATACCCTTGTTGCATTTTTCAGCAAAAATGGCTCCACAAACTGCGAAGAGTATCGATCTCTAGAGATTTATAACTTATCTGCTGCTGCTACTGCTAACTTGGAAGTTTTCAAGATAGAATCTTCTGGCTCAACAACATTAACCCAGGTCTGAATGAAGGTCCCCAGTTTTTTATGGTGTGAATCAAGAAGTGTAGTGGATAATCTTTTTGTTGATGTCTCTCACACCTA
TARGET=51,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04211 Ps_contig_mira-and-tgicl-ass_franssen_32486
SEQUENCE=GTCGRGATAACAAGTGATTTATTAAGCTCTGAATTGGGAGTGGAAGAGTCTGTGTGTGTGTTGGGGTTCTTTCTTCGCTTCCTTCCTATGGATCTTCATGCCGACTCTCCCAATTCCGGGATAAAGAAACGAAAACAAGACGAACAGGATGCTTTGGTCAGTGATCATGGAAATTCCATGAGTGTTGGAGAATCATCTGGTAGTGGTGACCCCTGCAAGAGTTTGACCTCTATATCATTGAGTTCAAAGAATTCAAATGGTTCCGATTCTGATATTTCATGTCATGACGATGATGAGGATGTGATTGATGATGATGATGACGCACTTGAAGATCCTGATTATGCATCTGATTTTTATGACGATGATGACGTCTATGAAGATGACTATTCACTTATGCAGGATCAATTTGACAATGTGGATTTACCTACTGGGGTAGAAGCATCACTACCGTGGTTGAAGGATATTGCTTCTAGCGAATGCAAGCCGGCTGTTGTTCATGCCCAGTCTGAGTCCAGTTCGAAAGAAAAAGTAGATGAGGCCACGGATGCCGTCATGCAAAAGTTTCGTCAATTTAAGCCATTTGATACTGTTGATTCTTTTCCTGATCATCACTATGATAAGGAAGGTGCTTCAGTCTTAGAGGCGCAGCGACCAAAGAATTGGGCCAAAAAAATTCAGGAGGAATGGAAAATTTTGGAGGAAAATTTGCCAGAAACAATCTTTGTGAGAGTTTGTGAATCTAAAATGGAGCTTCTTAGGGCTGTCATCATTGGACCTCAAGGCACTCCATACCATGATGGCCTATTCTTTTTCGATTGTTTATTTCCTTCTTCATACCCCGCAGGTCCACCGCAAGTTCACTATCACTCAGGTGGCCTAAGGCTAAATCCAAATCTGTATCAATGTGGAAAAGTGTGCCTTAGTCTCTTGGGTACTTGGCATGGTAAGAATAGCGAAAACTGGATTCCAGATAAATCAACCATGCTACAAGTCTTGGTTTCTATACAAGCTCTTATTCTGAATGAGAAACCTTTTTTTAATGAGCCTGGATATTCTGATGCATATCCTAGTGAGGAGGGGCAAAGGAGATCTAAGGATTACAATGATAATACATTTATTTTATCTTTGAAGACAATGATGTATACCCTGCGTAAACCGCCAAAGCACTTTGAGGACTTCGTTGCGGGGCATTTCCGTCAAAGGGGCGTGGACATACTGAAAGCATGTAGGTCATATGCTGAGGGGGGACCAGTTGGATCAGTTTTACATAATACTCGTAGTATTAATGCTTCTGCCAAGAACCAAAAAGAATTTCAGTCAACTGTGAGTAGGATGATGAATACCCTTGTTGCATTTTTCAGCAAAAATGGCTCCACAAACTGCGAAGAGTATCGATCTCTAGAGATTTATAACTTATCTGCTGCTGCTACTGCTAACTTGGAAGTTTTCAAGATAGAATCTTCTGGCTCAACAACATTAACCCAGGTCTGAATGAAGGTCCCCAGTTTTTTATGGTGTGAATCAAGAAGTGTAGTGGATAATCTTTTTGTTGATGTCTCTCACACCTA
TARGET=306,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04212 Ps_contig_mira-and-tgicl-ass_franssen_32488
SEQUENCE=AATGTAAACCCTAAAAACCCCGGCTCCGATTTCGANATTTTAGATAAGCTCGAATATCGAAATTGCGACTATAGGGTCCATTTTAGTCTTCATTCAAGTCTAGCGCAGCTTTGTGTCGTGTCGTGTTTTCGCAAACCAGAAAAACCAACTTCAAACGAGAGCTTCTTCCGATTCATCATTCTTCTTCTGCTATGGCTAATAATAATCGCCACACGATTATTCTAATCCAAACATCTCATAACAGAGCAACCAGAACTTTCAAGGATTATAACACAGTATCTCTAGCTATGGATGGCATATGTGCAATGTATGAAAGAAAGCTGAAGGAGTTGAATCCAGCCGTCAGAAATCTCTCTTATGATATTACGGATCTCTACAACTTCATAGACGGTCTTGCTGACATGAGTGCTCTAGTTTATGACAGTTCAATTCTTGCTTACTTGCCGTATGACCGAGAGTGGATTAAGCAACAGACGTTTCAACATTTGAAGAAATTGGCTCATTGATTGAACCCAAAGTTTTAGCAATTAGATGTATAAAGAAAATCACCCAGAGGGAGAACTTATCTGTTATGTGGGCTATCCTTTGATGGCTATGTTACTTAATATTATTATTGCACATACTGTTGGTGCGTTTTATATAATAGCTAGTGCCAGCGAAGTTTCGGAAACAAGCGATTTAAACCGGGCTTGTTTTATCCTTGTCAAGTGTTCTATATCTAGAAGACTCGGCCTTGTTAAAAAATCTTCATCTTGTACTATATAATAATAATAAGTTGCACTGTTTGTTTAACAACCT
TARGET=762,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04213 Ps_contig_mira-and-tgicl-ass_franssen_32490
SEQUENCE=TACGTCGGGGTATTCACAATAATTTCACACACACCAAGAATAATGGAATATAGAAGAAACTCATCATCATCATCATCATCAACATGCCTTCTATTCTCAATTCTTTCTTGTCTTTTTCTTTCATGTTTCTTAGGATCTGTTGGAGCTTACAAAAATTACACAGTGGGGGATTCTTTAGGCTGGTTTGACAAACTTGAAAAACCAACCGTTAATTATCAGAAATGGGTTGCCAAAAAAACAATTCAGCTTGGGAGATTTTCTCATTTTCAATACAGACAGCAATCACACTATTGTTCAAACATACAATTTCACAACATACAAACAATGTGACTACGATGATGCACAGGACAAAGACACACTCCAATGGTCATCAGGTTCTCAACCCAACGGTGAAGTAACTCCACTCACAGTGGCAGTTCCTTTGTTGAAAGAAGGACCAACATATTTCTTCTCGAGTGATTATGATGGCGAGCAATGCAAAAATGGACAACATTTCAGAATAAATGTGACTCATGGATTAGGGTTGCCAAAGAGTCTTCTACCAGATGATTCACCTTCTCCTGCTAGTCCGGTTTCCGGTGACGATGACTCGGCACCGGACACAACTGTTCCTTCCAATTTTAACAATCCTAAGGAAGACAAAGATGATGAATAAAACAAGTGATGAAGAAATCTTGGTTTCTTTATTCAGTTAGTTAAAGTATGCAACTAACTTAACAACTAAGTT
TARGET=61,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5688
PRIMER_LEFT_PENALTY=0.493727
PRIMER_RIGHT_PENALTY=0.075027
PRIMER_LEFT_SEQUENCE=ACGTCGGGGTATTCACAATAAT
PRIMER_RIGHT_SEQUENCE=GAAAATCTCCCAAGCTGAATTG
PRIMER_LEFT=1,22
PRIMER_RIGHT=259,22
PRIMER_LEFT_TM=59.506
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.8000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=0.7015
PRIMER_LEFT_1_PENALTY=0.493727
PRIMER_RIGHT_1_PENALTY=0.207756
PRIMER_LEFT_1_SEQUENCE=ACGTCGGGGTATTCACAATAAT
PRIMER_RIGHT_1_SEQUENCE=ATGAGAAAATCTCCCAAGCTGA
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=59.506
PRIMER_RIGHT_1_TM=60.208
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.7684
PRIMER_LEFT_2_PENALTY=0.493727
PRIMER_RIGHT_2_PENALTY=0.274705
PRIMER_LEFT_2_SEQUENCE=ACGTCGGGGTATTCACAATAAT
PRIMER_RIGHT_2_SEQUENCE=AATGAGAAAATCTCCCAAGCTG
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=264,22
PRIMER_LEFT_2_TM=59.506
PRIMER_RIGHT_2_TM=59.725
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=264
PRIMER_PAIR_PENALTY_3=0.8219
PRIMER_LEFT_3_PENALTY=0.746911
PRIMER_RIGHT_3_PENALTY=0.075027
PRIMER_LEFT_3_SEQUENCE=TACGTCGGGGTATTCACAATAA
PRIMER_RIGHT_3_SEQUENCE=GAAAATCTCCCAAGCTGAATTG
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=59.253
PRIMER_RIGHT_3_TM=60.075
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.9547
PRIMER_LEFT_4_PENALTY=0.746911
PRIMER_RIGHT_4_PENALTY=0.207756
PRIMER_LEFT_4_SEQUENCE=TACGTCGGGGTATTCACAATAA
PRIMER_RIGHT_4_SEQUENCE=ATGAGAAAATCTCCCAAGCTGA
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=263,22
PRIMER_LEFT_4_TM=59.253
PRIMER_RIGHT_4_TM=60.208
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_04214 Ps_contig_mira-and-tgicl-ass_franssen_32492
SEQUENCE=AAAGCTCCAGGAACACCTAGCATCCTGNTCYTTAGAAGCAGAAGAAGAAGAAGGTGCATCTGTTGCATCGGATTTGGGTACAGTTTCAAGGGCTTTGAAACCTTCAGAAGTCTTACCAACGGACAATCCCGAATCATACAAAAACTCCAACCTCTCCTGGTGAGGGACGAGACCAGCTTTTTCCTGAAGCTGACGAAACTCGCTACGTTCACGTTCCTCTTGAATCTGCTTACGAAGTTCATCGAGTTTCTTCTCCTCAGCGTTATGCTTCTGCTCTGCTTTCCAAACGTTTTCTATGTTCCTGAGACTTCCCGTGTGCCATCCTTTCTTGTTCAAAAACTTCAACGCCATTTTTTGTAATCTCTTCGGATTTGATTGGTTTCAGAGACAAAATACAACCCTGTTTCTTCAACGTTGCTTGCAACTTGGAGAAGAAAATCATAAAAGGTGAAGCGATGAATTGCAATTGGGTCTTATGGTTCAAGACGGGTTCGG
TARGET=40,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04215 Ps_contig_mira-and-tgicl-ass_franssen_32493
SEQUENCE=CCTTTCCTTCAACTGATTTTCTAATGGTTTCCTATCTTGACAGGGTTGTTTTTAATCTTTGCAAGTGCTTCCTGTTCGCGCTGGCGAATCATCAGCAAGGGATCAGAATGGAGCTTTCTCCAAGCATCATTAGCAGACTGAGGCTTGTCCTCAAATAAAGCTCCAGGAACACCTGCATCCTGCTTAGAAGCAGAAGAAGAAGAAGGTGCATCTGTTGCATCGGATTTGGGTACAGTTTCAAGGGCTTTGAAACCTTCAGAAGTCTTACCAACGGACAATCCCGAATCATACAAAAACTCCAACCTCTCCTG
TARGET=192,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1846
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.183834
PRIMER_LEFT_SEQUENCE=CAAATAAAGCTCCAGGAACACC
PRIMER_RIGHT_SEQUENCE=GGAGTTTTTGTATGATTCGGGA
PRIMER_LEFT=151,22
PRIMER_RIGHT=299,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.184
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=0.2524
PRIMER_LEFT_1_PENALTY=0.068521
PRIMER_RIGHT_1_PENALTY=0.183834
PRIMER_LEFT_1_SEQUENCE=ATCATCAGCAAGGGATCAGAAT
PRIMER_RIGHT_1_SEQUENCE=GGAGTTTTTGTATGATTCGGGA
PRIMER_LEFT_1=87,22
PRIMER_RIGHT_1=299,22
PRIMER_LEFT_1_TM=59.931
PRIMER_RIGHT_1_TM=60.184
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.2705
PRIMER_LEFT_2_PENALTY=0.086698
PRIMER_RIGHT_2_PENALTY=0.183834
PRIMER_LEFT_2_SEQUENCE=ATTAGCAGACTGAGGCTTGTCC
PRIMER_RIGHT_2_SEQUENCE=GGAGTTTTTGTATGATTCGGGA
PRIMER_LEFT_2=128,22
PRIMER_RIGHT_2=299,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=60.184
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=0.3199
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.183834
PRIMER_LEFT_3_SEQUENCE=TGGTTTCCTATCTTGACAGGGT
PRIMER_RIGHT_3_SEQUENCE=GGAGTTTTTGTATGATTCGGGA
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=299,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=60.184
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.3274
PRIMER_LEFT_4_PENALTY=0.068521
PRIMER_RIGHT_4_PENALTY=0.258908
PRIMER_LEFT_4_SEQUENCE=ATCATCAGCAAGGGATCAGAAT
PRIMER_RIGHT_4_SEQUENCE=CCTTGAAACTGTACCCAAATCC
PRIMER_LEFT_4=87,22
PRIMER_RIGHT_4=242,22
PRIMER_LEFT_4_TM=59.931
PRIMER_RIGHT_4_TM=59.741
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=156
=
PRIMER_SEQUENCE_ID=SSR_04216 Ps_contig_mira-and-tgicl-ass_franssen_32501
SEQUENCE=AAGGTTTTANNTNTAAGTACCAACACACTTTCACCTTTTATTCTGATAACAAATGAGGAATAGAAATATGCAGCAGCACAATGAAAAGGCTGTTCAATTCAACTCATACATACTCCTACCTAAAACTCAACCTTTCCTGTCAAATAAAGATGCAAGCCCTTATATCTAAATTTACATGTAATCAGTGCAAATTCATTACAAAACATAAGATTTTACAAAAAAAAAAGATGATAAATGACATATTGTAAAACAAAAAAGAAGCATCGTGGTTCCTACCTCAGCTATCAATATCTTCTATCACAGTTGCACACAGTCATCACCCCAATGTAGTGTTGTTGTATATGAATATTATTACGACTGCACGGCTTTATTTTGTTTGATACAAGCCGGTACCTTGAAGTCCTGGCATTTCCATGTTGATGTAGCTTCGATCGGCGTCATTCGGTCAGTCGATGACTCCACCGGAAACACGGGTATAGTATGAACATGCTGATTGTCTTTGTTCAGTTCGGTCAATGAAGAACTTCTTCTGACTTTTATTTCTTCCCTCGGCTGTGCTTTATCTTTCGAGGTGCAGATGCTTAAGGGTAACTTTTTTAGCTTTGTTCTTCCCCTCTTCTTACCTATGATGGAAGGTGAATGTGAGTCTTCACAGTTTTTCGGTTTTGAGTGGCTCGACTCTGCTTCCCCGTTATCTATCTCCATTTGGCTAGACCTATCGAATTTGATTACATTCTTCTTATCTTTCTTACTAGAGCATACCTCTTGGTTGGATCCTTCGGAGTCAATCTTTCCGACAACTGCTTGGCCGTTGTTGTTTAAAGATAAACAATCTTGTCCAACCGCCATGCCACATTCGGAGCCTTCGGTTATTTCAAAACTCGAAGAGCTTCTAGGCCTCTTCGATCGACTACCTTTTGAGTCAGATTTCGGAGAGTTCTTAGAGATTCTCGGAGAACTATTCATGGCTCGGTGTAACTTTCGAGCTAACTCTTCATCTTTCCTACAATTTTCAATCGGTTGTTTTTTTTGTACAACAGCTGAACTGGCACATGCTTCTTTGACTTGTTCTTTTTCAGATTCCTTTCCTTGTTGACGATTGCATCCTCGGAAAACGAAGCAACCAAATCTAAAGCACTCTTGGCCTTAGTAACCGCCTTCGCTGCTATCGCAGCTTTTTCTTCAGCTGCAGTTCTTGCTGCCTCGGCAGCCTTAGTTGCAGTAACCGCTGCTTTTCTCGAAGCCTCCGCGACCTCCTCAGGCGTAAGGTTAGGGTTATTTGAATGACAATTCAGCAAATGCTTAAGCCGAGCTTCAAATTCACTTGAAAAGGACGCAGATGAAGGGGAAGCAACATCTGTAACACAAGAAGCAGCAACCGGTGACGGTGACGCAGTAGCATCACTTTCTGAACTCTGTTTTTTAAGTCCAGCAAGAAGCCGTTTCCGAGGTGGCAAAAGAACATCAGCATCCAACTGATTCACATCGCACGCGTTCTCCATCCTCCCCCAATAAATTCAATTCAATACTCACAAACAAAATTTATATCATTCACATGAATTAAATTGCGATTCCATTAACAATCATATCAACAGCACAACGATCCAACTTTTCCCAACTCCGCATCTTCGACGAAATCAAAATTTAAACAAACCTACAAGATCCTTAACAAACAAACCCTACATAAAACTTATACCAATTCAAACACTATCCAAGGTGGTACCATTTTCTATCATCAAAAAACCTCTATAAGAAGAAAAAACATAACTCATACCCAACATCAATACCTTCCATTTCCGATTCCACAACTTTAATTCCTGATCCTGTTGATGATGGTGAAGGAAGGTAGAGATAGAGGAGTAAAGGAAGATCCAGAATTAGGGTTTCGAGAGAGAGAAAACAAGAGGTTGTGATGGTGAAGAAGAAAATCTAAGGTCTGTAAAAACCCAACNCCTTTGTTAT
TARGET=1888,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2034
PRIMER_LEFT_PENALTY=0.054900
PRIMER_RIGHT_PENALTY=0.148509
PRIMER_LEFT_SEQUENCE=CCCAACATCAATACCTTCCATT
PRIMER_RIGHT_SEQUENCE=TCTTCACCATCACAACCTCTTG
PRIMER_LEFT=1773,22
PRIMER_RIGHT=1921,22
PRIMER_LEFT_TM=59.945
PRIMER_RIGHT_TM=60.149
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=0.2235
PRIMER_LEFT_1_PENALTY=0.075027
PRIMER_RIGHT_1_PENALTY=0.148509
PRIMER_LEFT_1_SEQUENCE=ACATCAATACCTTCCATTTCCG
PRIMER_RIGHT_1_SEQUENCE=TCTTCACCATCACAACCTCTTG
PRIMER_LEFT_1=1777,22
PRIMER_RIGHT_1=1921,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=0.2364
PRIMER_LEFT_2_PENALTY=0.054900
PRIMER_RIGHT_2_PENALTY=0.181485
PRIMER_LEFT_2_SEQUENCE=CCCAACATCAATACCTTCCATT
PRIMER_RIGHT_2_SEQUENCE=CAAAGGNGTTGGGTTTTTACAG
PRIMER_LEFT_2=1773,22
PRIMER_RIGHT_2=1957,22
PRIMER_LEFT_2_TM=59.945
PRIMER_RIGHT_2_TM=60.181
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.2565
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.181485
PRIMER_LEFT_3_SEQUENCE=ACATCAATACCTTCCATTTCCG
PRIMER_RIGHT_3_SEQUENCE=CAAAGGNGTTGGGTTTTTACAG
PRIMER_LEFT_3=1777,22
PRIMER_RIGHT_3=1957,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=60.181
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.75
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.4147
PRIMER_LEFT_4_PENALTY=0.233224
PRIMER_RIGHT_4_PENALTY=0.181485
PRIMER_LEFT_4_SEQUENCE=GTTGATGATGGTGAAGGAAGGT
PRIMER_RIGHT_4_SEQUENCE=CAAAGGNGTTGGGTTTTTACAG
PRIMER_LEFT_4=1825,22
PRIMER_RIGHT_4=1957,22
PRIMER_LEFT_4_TM=60.233
PRIMER_RIGHT_4_TM=60.181
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_04217 Ps_contig_mira-and-tgicl-ass_franssen_32503
SEQUENCE=GTGAAAATATTTACACTTGGATTAACTTTTCCAATTATTATGTCAAAATAAAGCAGCAATAATAATAAAAACAGTACAAGAAAACAAAACTCCACTAGTTTCTGTTTTCCAACTTGAATTGAAAAACACCATAAAATTGAAATAAAAGCAAAGACAAGAAATAATAATAACAAAAATAGAAATGAAAGAATCTCCAACATTACATTACATAAACCCCATCTAGTTAAAGCGGACACCAACACCATCAAGCACTGTTTCTTTCTTCTCTCTGATATTGATATCACTGAGTGTTGCACATGTGTCTCACTGAATCCCAATCATACAATTTTGAGTGGTCCCATTCTATAAAGTGGAACACATCACGTGACTGCCACAGTTTTGATAGCAAGAGCTTACCACTAATATTCTGCCTAATATCTTTTTGCTTTTACCTTCATTACTCCTTCTAGCAAAACTATGCTGTTTCTTGAATAAAAAAAGAAATGACAGCATAGAATCATGTGAACTATAGCAGCTAGGTCTCAGACATTGATAAGATGTTCATTTTCAATGTGAGTATCATTATCACAAGGTGGTTTAGGTGATGGAGATGGTGAAGCATTATTCTTGATGTTTTGTTGATGATAAACTTGTCTCAGCCTTTCTATCTCCCTCTTCAATGCCTCTTGATGAGCATCTTTGAAGATTTTGTCTTGGGCAAGAGCTGCGATTCTTTGCTTGAGAGCACTGTTATCAACATTTAGAAGCAACCTCTGATGATCCAGAAATGCTACTCGTGGTGACAGTACTGATACTTCTGCCTGTAATGAAGTTACACTTCTCTCAAGCTCTGATATGTATTGCAGTTTCCTCACTCTTGATCTCTGTGCGGATTGTCTATTTGCCAAGATTCTTTTGACCCTCTTGGGGTCAGTAATTTTCTGACTTGAATAAGATGTGTTTCCGTTAGTATCTTCATCCTCATCATTGACGACTCGTGCGGTTTCCTGTTTGCATTCATCAAATTCATCCTCATCAGATTCATGCTTCAATTGTTTCTGTTGTTGATGATGCTTTTCATCCTCCTCCTCCTCTTCATTGATGCTGTTATTATCAGAAGGAGTTGAAGGGTTTGAGTTAGAGTACAATGTAGCTGCTCCGGCTGCGGCTGCGGCTGGCGGCTGCATCATGTTTTTCCCGGAAACCTCATCCACGCCAAACATAGACATCAATTGTTCATCATCAAACCTTTCAAACTCACCGTCCCTGTCTCTACGGCGACAATGCTCCATCATTGACGCCTCGATGAACGTCACAGAATCACTCGCCGACCGTCTGTGAGCCCCTCGTCTGGTGGACGAGAAGTCGAGAAACTCGTCCACCCATGAAGGGTTTTGTTGACTGTTGGAAAAGTTTGAAGCATTGAGTGGTGGAATAGTTTTAAGGTTAGGCATTTTTTGATGAGAAGAAAATTCAGGCCAAGGTTGTGTCATGTTTGGAATTGGAACTTTGGGTGGCAATTGAGCCATTTGAGAGATAAGCTATAAAACTCAATATAGCTACAATGGAATAGACTCAAGAAAATGAAAGTGTGGTGTTATTTATATATATGCAATGTGTGAATATGTATTATTGTGTGTCAAGTAGACAAAAAGAAACGAAGAAACATGTGTGTATGAATGTGAAGGAGCCAGAGGGAATGTTAGAAAGGCAGAAGAAGAAGAAGAATATGAACTGAATTCAATATACTAGTGATATTGTTTATCAYYGNTATGGGGATTGAAATGACAATAAATAAAAAAATAAATGTGTGGGG
TARGET=1060,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04218 Ps_contig_mira-and-tgicl-ass_franssen_32503
SEQUENCE=GTGAAAATATTTACACTTGGATTAACTTTTCCAATTATTATGTCAAAATAAAGCAGCAATAATAATAAAAACAGTACAAGAAAACAAAACTCCACTAGTTTCTGTTTTCCAACTTGAATTGAAAAACACCATAAAATTGAAATAAAAGCAAAGACAAGAAATAATAATAACAAAAATAGAAATGAAAGAATCTCCAACATTACATTACATAAACCCCATCTAGTTAAAGCGGACACCAACACCATCAAGCACTGTTTCTTTCTTCTCTCTGATATTGATATCACTGAGTGTTGCACATGTGTCTCACTGAATCCCAATCATACAATTTTGAGTGGTCCCATTCTATAAAGTGGAACACATCACGTGACTGCCACAGTTTTGATAGCAAGAGCTTACCACTAATATTCTGCCTAATATCTTTTTGCTTTTACCTTCATTACTCCTTCTAGCAAAACTATGCTGTTTCTTGAATAAAAAAAGAAATGACAGCATAGAATCATGTGAACTATAGCAGCTAGGTCTCAGACATTGATAAGATGTTCATTTTCAATGTGAGTATCATTATCACAAGGTGGTTTAGGTGATGGAGATGGTGAAGCATTATTCTTGATGTTTTGTTGATGATAAACTTGTCTCAGCCTTTCTATCTCCCTCTTCAATGCCTCTTGATGAGCATCTTTGAAGATTTTGTCTTGGGCAAGAGCTGCGATTCTTTGCTTGAGAGCACTGTTATCAACATTTAGAAGCAACCTCTGATGATCCAGAAATGCTACTCGTGGTGACAGTACTGATACTTCTGCCTGTAATGAAGTTACACTTCTCTCAAGCTCTGATATGTATTGCAGTTTCCTCACTCTTGATCTCTGTGCGGATTGTCTATTTGCCAAGATTCTTTTGACCCTCTTGGGGTCAGTAATTTTCTGACTTGAATAAGATGTGTTTCCGTTAGTATCTTCATCCTCATCATTGACGACTCGTGCGGTTTCCTGTTTGCATTCATCAAATTCATCCTCATCAGATTCATGCTTCAATTGTTTCTGTTGTTGATGATGCTTTTCATCCTCCTCCTCCTCTTCATTGATGCTGTTATTATCAGAAGGAGTTGAAGGGTTTGAGTTAGAGTACAATGTAGCTGCTCCGGCTGCGGCTGCGGCTGGCGGCTGCATCATGTTTTTCCCGGAAACCTCATCCACGCCAAACATAGACATCAATTGTTCATCATCAAACCTTTCAAACTCACCGTCCCTGTCTCTACGGCGACAATGCTCCATCATTGACGCCTCGATGAACGTCACAGAATCACTCGCCGACCGTCTGTGAGCCCCTCGTCTGGTGGACGAGAAGTCGAGAAACTCGTCCACCCATGAAGGGTTTTGTTGACTGTTGGAAAAGTTTGAAGCATTGAGTGGTGGAATAGTTTTAAGGTTAGGCATTTTTTGATGAGAAGAAAATTCAGGCCAAGGTTGTGTCATGTTTGGAATTGGAACTTTGGGTGGCAATTGAGCCATTTGAGAGATAAGCTATAAAACTCAATATAGCTACAATGGAATAGACTCAAGAAAATGAAAGTGTGGTGTTATTTATATATATGCAATGTGTGAATATGTATTATTGTGTGTCAAGTAGACAAAAAGAAACGAAGAAACATGTGTGTATGAATGTGAAGGAGCCAGAGGGAATGTTAGAAAGGCAGAAGAAGAAGAAGAATATGAACTGAATTCAATATACTAGTGATATTGTTTATCAYYGNTATGGGGATTGAAATGACAATAAATAAAAAAATAAATGTGTGGGG
TARGET=1692,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04219 Ps_contig_mira-and-tgicl-ass_franssen_32510
SEQUENCE=GGGAAAGTGCTTTTCATTTCTGCGTTAGAATCTGAGAGAAGAAAAAGAGAAGAAGAAGAAAGAGGAGACACAGACACAAATCAATCAACTATAGGTATTTTCACGTCTGTGGCTCCTCTTAATATAAAAGCTAGTTAGTTTCTGGGTTAAGAAAATTTAAAGGAAAAGATGGATTATGAGAGTAATAGTTGGATTTGGGAAGGGGTGTACTACTATCCACATTTATTTGGTGGTTTAATGGTTACGGCGGCTTTGTTGGGCTTGTCAACAAGCTACTTTGGTGTGATTGGGGTTCCTTATTTGCCGCTTCCACACTCTTGGTACAATTTGGGGGTATTCCGCAAGAAGAAGAACGTTGGGAAGAGGCGCGTTCGGGTTTACATGGATGGGTGTTTTGATCTGATGCATTATGGCCATGCTAATGCACTCAGACAAGCTAAAGCTTTAGGTGATGAGTTGGTTGTTGGGCTTGTCAGCGATGAGGAGATTGTGGCCAATAAAGGACCCCCTGTTTTGTCCATGGATGAGAGACTGGCCCTAGTCAGTGGATTAAAGTGGGTGGATGAGGTCATAACTGATGCTCCTTATGCAATAACTGAAAATTTCTTGAACCGTCTCTTTCATGAATACAACATTGACTATGTCATACATGGCGATGATCCTTGCCTGCTTCCTGACGGAACAGATGCTTATGCTGCGGCAAAGAAAGCTGGCCGTTACAAACAAATTAAACGTACTGAAGGAGTCTCTAGTACGGATATCGTAGGAAGAATACTGTCTTCTTTAAAAGAACAAAACAATCGTGAAGATGTAAAACCCCAAGACGAATGCCAGTCAAAGGTTTCCCATATATCCCAATTCCTACCAACTTCCCGACGTATTGTGCAGTTTTCAAATGGCAAGGGTCCAAGGCCAAATGCTCGTATTGTATACATTGATGGAGCATTTGATCTCTTCCATGCAGGTCATGTTCAGATGCTTAAGAGAGCTAGGGAACTTGGAGATTTTCTTTTAGTTGGTATTCACTCTGATGAGACAGTGAGTGAAAATCGAGGAAATCACTATCCAATTATGCATCTACATGAGCGGAGCCTTAGTGTGTTAGCTTGCCGTTATGTTGACGAAGTCATTATTGGTGCACCCTGGGAAATTACAAAGGACATGATCACAACTTTCAATATCTCACTAGTTGTTCACGGCACTGTTGCTGAGAAGTCATTACCTAGTGAAAAAGATCCTTATGAAGTTCCTAAGAGCATTGGCAAATTCCGCTTGCTTGAAAGCCCAAAAGATATTACTACTACCTCAGTAGCTCAAAGGATAATGGCTAATCATGATGCCTATGTGCAACGGAATGCTAAGAAAGCCAAGAGTGAGAAGAGATACTATGAAGAAAGAAAATACGTTTCTGGTGACTAGCATTGTATAGACTTAGTTTGTTACTGGCCTACAAGCATAACCCTGTGAGAGATACTTGATATAGCAGGAAATCTATACATTTGTTGAAGGATCATTAGTACAGTGTATGACATAGAATGAGATTATTGTAGATTACTTGGGAATNCCATTGTACTATAGTGTCCCTACACATTTTTTTCTTTGTGATTTGAAAGGCTCCAGTGGGGTTAGCCCCACAAGTTTTATGGCATCTGTATTTTTGCTCTGGTATCGATTTATTTATTTTGTGTATGTATTCATTAT
TARGET=48,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2529
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=GGGAAAGTGCTTTTCATTTCTG
PRIMER_RIGHT_SEQUENCE=AACCCCAATCACACCAAAGTAG
PRIMER_LEFT=0,22
PRIMER_RIGHT=293,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=0.3879
PRIMER_LEFT_1_PENALTY=0.243965
PRIMER_RIGHT_1_PENALTY=0.143968
PRIMER_LEFT_1_SEQUENCE=GGAAAGTGCTTTTCATTTCTGC
PRIMER_RIGHT_1_SEQUENCE=AACCCCAATCACACCAAAGTAG
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=293,22
PRIMER_LEFT_1_TM=60.244
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.8834
PRIMER_LEFT_2_PENALTY=0.108929
PRIMER_RIGHT_2_PENALTY=0.774490
PRIMER_LEFT_2_SEQUENCE=GGGAAAGTGCTTTTCATTTCTG
PRIMER_RIGHT_2_SEQUENCE=CCCTTCCCAAATCCAACTATTA
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=203,22
PRIMER_LEFT_2_TM=60.109
PRIMER_RIGHT_2_TM=59.226
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=204
PRIMER_PAIR_PENALTY_3=0.9245
PRIMER_LEFT_3_PENALTY=0.243965
PRIMER_RIGHT_3_PENALTY=0.680486
PRIMER_LEFT_3_SEQUENCE=GGAAAGTGCTTTTCATTTCTGC
PRIMER_RIGHT_3_SEQUENCE=ATTAAGAGGAGCCACAGACGTG
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=122,22
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=60.680
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=0.9339
PRIMER_LEFT_4_PENALTY=0.108929
PRIMER_RIGHT_4_PENALTY=0.824935
PRIMER_LEFT_4_SEQUENCE=GGGAAAGTGCTTTTCATTTCTG
PRIMER_RIGHT_4_SEQUENCE=CCGTAACCATTAAACCACCAAA
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=60.825
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_04220 Ps_contig_mira-and-tgicl-ass_franssen_32510
SEQUENCE=GGGAAAGTGCTTTTCATTTCTGCGTTAGAATCTGAGAGAAGAAAAAGAGAAGAAGAAGAAAGAGGAGACACAGACACAAATCAATCAACTATAGGTATTTTCACGTCTGTGGCTCCTCTTAATATAAAAGCTAGTTAGTTTCTGGGTTAAGAAAATTTAAAGGAAAAGATGGATTATGAGAGTAATAGTTGGATTTGGGAAGGGGTGTACTACTATCCACATTTATTTGGTGGTTTAATGGTTACGGCGGCTTTGTTGGGCTTGTCAACAAGCTACTTTGGTGTGATTGGGGTTCCTTATTTGCCGCTTCCACACTCTTGGTACAATTTGGGGGTATTCCGCAAGAAGAAGAACGTTGGGAAGAGGCGCGTTCGGGTTTACATGGATGGGTGTTTTGATCTGATGCATTATGGCCATGCTAATGCACTCAGACAAGCTAAAGCTTTAGGTGATGAGTTGGTTGTTGGGCTTGTCAGCGATGAGGAGATTGTGGCCAATAAAGGACCCCCTGTTTTGTCCATGGATGAGAGACTGGCCCTAGTCAGTGGATTAAAGTGGGTGGATGAGGTCATAACTGATGCTCCTTATGCAATAACTGAAAATTTCTTGAACCGTCTCTTTCATGAATACAACATTGACTATGTCATACATGGCGATGATCCTTGCCTGCTTCCTGACGGAACAGATGCTTATGCTGCGGCAAAGAAAGCTGGCCGTTACAAACAAATTAAACGTACTGAAGGAGTCTCTAGTACGGATATCGTAGGAAGAATACTGTCTTCTTTAAAAGAACAAAACAATCGTGAAGATGTAAAACCCCAAGACGAATGCCAGTCAAAGGTTTCCCATATATCCCAATTCCTACCAACTTCCCGACGTATTGTGCAGTTTTCAAATGGCAAGGGTCCAAGGCCAAATGCTCGTATTGTATACATTGATGGAGCATTTGATCTCTTCCATGCAGGTCATGTTCAGATGCTTAAGAGAGCTAGGGAACTTGGAGATTTTCTTTTAGTTGGTATTCACTCTGATGAGACAGTGAGTGAAAATCGAGGAAATCACTATCCAATTATGCATCTACATGAGCGGAGCCTTAGTGTGTTAGCTTGCCGTTATGTTGACGAAGTCATTATTGGTGCACCCTGGGAAATTACAAAGGACATGATCACAACTTTCAATATCTCACTAGTTGTTCACGGCACTGTTGCTGAGAAGTCATTACCTAGTGAAAAAGATCCTTATGAAGTTCCTAAGAGCATTGGCAAATTCCGCTTGCTTGAAAGCCCAAAAGATATTACTACTACCTCAGTAGCTCAAAGGATAATGGCTAATCATGATGCCTATGTGCAACGGAATGCTAAGAAAGCCAAGAGTGAGAAGAGATACTATGAAGAAAGAAAATACGTTTCTGGTGACTAGCATTGTATAGACTTAGTTTGTTACTGGCCTACAAGCATAACCCTGTGAGAGATACTTGATATAGCAGGAAATCTATACATTTGTTGAAGGATCATTAGTACAGTGTATGACATAGAATGAGATTATTGTAGATTACTTGGGAATNCCATTGTACTATAGTGTCCCTACACATTTTTTTCTTTGTGATTTGAAAGGCTCCAGTGGGGTTAGCCCCACAAGTTTTATGGCATCTGTATTTTTGCTCTGGTATCGATTTATTTATTTTGTGTATGTATTCATTAT
TARGET=1672,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04221 Ps_contig_mira-and-tgicl-ass_franssen_32512
SEQUENCE=TTAATGTTATTCTGCCAACGATGCATTCTTATTTTTCTATTTATTGTTGGGCTAAAGTAAACAACTTAATGCATTACAAATACACGATACTTTATCACAGATAGTTTCACATATATATATATAAATACATACAAGAATCCATAAAGTAAACCTTCACGTATATAAATCCATAAGAGCTTATTTACAACACATACTAAAAGCTCATAAAAGATATTCCTACATAATAATAATCTACCAGAATCTCACTAGGCAGCTTCAGCAAAACCAAGTTGAAGATTTCCATAGTCAAAGATAGTGTGATATACCCTCATAAAAATATCACCAAGAATCCATAGAGGACCCCTTGGAGGAGGAATGTCAAACGCAATAAACCCACTAAGACAGACTTGTGTAATACCTTCTCCGGTTTTCAAAATATACTGCTCGGGACTGAGGACGAAAGATTTATCTCCGATGGTAAACGAAATGTTTGGCAATTGATAAATGCTATTGCAGTCTACTACGGACTCTCCATTTGGACTTGGCAAGCTCTCGCACAGTTGATTTACGTAGTTGAATATTCTATCTTTGGTTGTCTTTCGCTTTAGTTGATTCTGGATCCAAATCACAAGCATTTGACAGGAAGAGCACAAAGCAGTATCTTTAGCAGANACTCTCCTCTTCTTCGCACATCTCAACTTCCCATCGTCTTCTTTGTATTGACTTCTTCGTTT
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0591
PRIMER_LEFT_PENALTY=1.019196
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=ATGTTATTCTGCCAACGATGC
PRIMER_RIGHT_SEQUENCE=AAGCTGCCTAGTGAGATTCTGG
PRIMER_LEFT=3,21
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=59.981
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=1.1498
PRIMER_LEFT_1_PENALTY=1.109878
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=CTGCCAACGATGCATTCTTAT
PRIMER_RIGHT_1_SEQUENCE=AAGCTGCCTAGTGAGATTCTGG
PRIMER_LEFT_1=11,21
PRIMER_RIGHT_1=255,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=1.2598
PRIMER_LEFT_2_PENALTY=1.219886
PRIMER_RIGHT_2_PENALTY=0.039879
PRIMER_LEFT_2_SEQUENCE=AATGTTATTCTGCCAACGATGC
PRIMER_RIGHT_2_SEQUENCE=AAGCTGCCTAGTGAGATTCTGG
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=61.220
PRIMER_RIGHT_2_TM=60.040
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=1.3732
PRIMER_LEFT_3_PENALTY=1.333370
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=CTGCCAACGATGCATTCTTATT
PRIMER_RIGHT_3_SEQUENCE=AAGCTGCCTAGTGAGATTCTGG
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=61.333
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=1.4672
PRIMER_LEFT_4_PENALTY=1.019196
PRIMER_RIGHT_4_PENALTY=0.447971
PRIMER_LEFT_4_SEQUENCE=ATGTTATTCTGCCAACGATGC
PRIMER_RIGHT_4_SEQUENCE=AGCTGCCTAGTGAGATTCTGGT
PRIMER_LEFT_4=3,21
PRIMER_RIGHT_4=254,22
PRIMER_LEFT_4_TM=59.981
PRIMER_RIGHT_4_TM=59.552
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_04222 Ps_contig_mira-and-tgicl-ass_franssen_32513
SEQUENCE=TAACACGAAGCTCACACCCATGGCAATGAGGAAAAACTGCATCCTTGTAAGTCCTTTTGATTTTTCATCTTTTTTCATGGAGAGCCCTAAAAGAGAGAGACTTGTGCAAGGTTTGCTGGCCACCACATTTCAATTGGGTCCACCAGATACCTCCTCAGAATCCCAGCCCATCCATACCCTATTAACTGTGTGCTTATGACGATGAAAAGAGCAAGAAGAAAACTAAGAGATTGTTTATAATAAGCTTTCATAATAGTAATGGCACCAATGGAATAAGCATCACCACCACCACTGGAAACACCA
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04223 Ps_contig_mira-and-tgicl-ass_franssen_32518
SEQUENCE=TCGGGTTGGCTTCCCTCTGTTTGCGGAAATCTCCCCTGACCTGACCTGATAGATGTCGGCAAGCCCCTAAAGTTCCGTCCACATCCGGCCCACCACCACCACTTCTCCGCTCTCGATCCAAATTCCGTCGTTAGCATTTTGCAAGACAACGGTGTTCTAGAGTTTTAGGTTTATTTATTCGATTCAAAGAAATGCGAAGGGTATACTTTTATTTCTCTGTATTGTGTGATTGTAGTAGGTAAGAAGAAATCAGAAAAAATATATATAAAAATGGTTTCAGAAATGAGTGACTTATCCATCTCCAAGAATGATCGTGCTTCCCATTCCAAATTCATTACTCTGCCCACCATTTTGACGCTCGGCCGTGTCGCATCTATTCCTCTTCTCGTTGCCACCTTCTATATGGATGGTTGGCAAGGAACTGCTATTACCACAAGTATTTTTACTGCCGCTGCAATTACAGATTGGCTTGATGGCTATATTGCTCGCAAGATGAAATTAAAATCTTCGTTTGGTGCGTTTTTAGATCCAGTAGCGGACAAGCTTATGGTTGCTGCCACGTTGATCTTGTTATGTACTAGACCGGTCAAAGTTGCTGCATTGGCGCATGCTCCATGGCTCTTGATTATACCTTCCATCATCATAATTGGTAGAGAGATAACCATGTCGGCACTCAGGGAATGGGCTGCTTCCCAGGGTAGTAAAACTTTAGAGGCTGTTGCGGTTAATAGTTTGGGGAAATGGAAAACAGCCACGCAAATGACAGCATTAACCATCCTTCTTGCTACCCGTGACTGCAGTCATGGAGGACCTGCGATTTTGGCAGGCTCTGGTGTTTTGTTGCTTTATATTGCGGCAGGGCTAGCCGTATGGTCGTTTGTAGTATATATGAGGAAAATATGGAAGGTGTTAGTGAGGTAACATGGACGACGACGGGTCTTGGTTTCTTTGATGGGTTTGGTTTTCACGAATCACGACCATTATTATTTCTTTCAAAAATAGTTTAGTCAGTGATACCGTGGAAGGGAGACGCAGTTCTCGATTCTCATGACATATATTTGTAATATTTACTATCTTCATTGTCTAACTCTGCAAACCACCATTGGTTCCATTTATGAAAATTATATGCACAATTTTTTTTGAAGTT
TARGET=35,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.6806
PRIMER_LEFT_PENALTY=3.502415
PRIMER_RIGHT_PENALTY=0.178157
PRIMER_LEFT_SEQUENCE=CCCTCTGTTTGCGGAAATCT
PRIMER_RIGHT_SEQUENCE=GTTGTCTTGCAAAATGCTAACG
PRIMER_LEFT=12,20
PRIMER_RIGHT=149,22
PRIMER_LEFT_TM=61.502
PRIMER_RIGHT_TM=59.822
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=138
PRIMER_PAIR_PENALTY_1=3.6806
PRIMER_LEFT_1_PENALTY=3.502415
PRIMER_RIGHT_1_PENALTY=0.178157
PRIMER_LEFT_1_SEQUENCE=CCCTCTGTTTGCGGAAATCT
PRIMER_RIGHT_1_SEQUENCE=CGTTGTCTTGCAAAATGCTAAC
PRIMER_LEFT_1=12,20
PRIMER_RIGHT_1=150,22
PRIMER_LEFT_1_TM=61.502
PRIMER_RIGHT_1_TM=59.822
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=139
PRIMER_PAIR_PENALTY_2=4.1193
PRIMER_LEFT_2_PENALTY=3.941143
PRIMER_RIGHT_2_PENALTY=0.178157
PRIMER_LEFT_2_SEQUENCE=GGTTGGCTTCCCTCTGTTTG
PRIMER_RIGHT_2_SEQUENCE=GTTGTCTTGCAAAATGCTAACG
PRIMER_LEFT_2=3,20
PRIMER_RIGHT_2=149,22
PRIMER_LEFT_2_TM=61.941
PRIMER_RIGHT_2_TM=59.822
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=4.1193
PRIMER_LEFT_3_PENALTY=3.941143
PRIMER_RIGHT_3_PENALTY=0.178157
PRIMER_LEFT_3_SEQUENCE=GGTTGGCTTCCCTCTGTTTG
PRIMER_RIGHT_3_SEQUENCE=CGTTGTCTTGCAAAATGCTAAC
PRIMER_LEFT_3=3,20
PRIMER_RIGHT_3=150,22
PRIMER_LEFT_3_TM=61.941
PRIMER_RIGHT_3_TM=59.822
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=4.2659
PRIMER_LEFT_4_PENALTY=4.087731
PRIMER_RIGHT_4_PENALTY=0.178157
PRIMER_LEFT_4_SEQUENCE=GTTGGCTTCCCTCTGTTTGC
PRIMER_RIGHT_4_SEQUENCE=GTTGTCTTGCAAAATGCTAACG
PRIMER_LEFT_4=4,20
PRIMER_RIGHT_4=149,22
PRIMER_LEFT_4_TM=62.088
PRIMER_RIGHT_4_TM=59.822
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_04224 Ps_contig_mira-and-tgicl-ass_franssen_32518
SEQUENCE=TCGGGTTGGCTTCCCTCTGTTTGCGGAAATCTCCCCTGACCTGACCTGATAGATGTCGGCAAGCCCCTAAAGTTCCGTCCACATCCGGCCCACCACCACCACTTCTCCGCTCTCGATCCAAATTCCGTCGTTAGCATTTTGCAAGACAACGGTGTTCTAGAGTTTTAGGTTTATTTATTCGATTCAAAGAAATGCGAAGGGTATACTTTTATTTCTCTGTATTGTGTGATTGTAGTAGGTAAGAAGAAATCAGAAAAAATATATATAAAAATGGTTTCAGAAATGAGTGACTTATCCATCTCCAAGAATGATCGTGCTTCCCATTCCAAATTCATTACTCTGCCCACCATTTTGACGCTCGGCCGTGTCGCATCTATTCCTCTTCTCGTTGCCACCTTCTATATGGATGGTTGGCAAGGAACTGCTATTACCACAAGTATTTTTACTGCCGCTGCAATTACAGATTGGCTTGATGGCTATATTGCTCGCAAGATGAAATTAAAATCTTCGTTTGGTGCGTTTTTAGATCCAGTAGCGGACAAGCTTATGGTTGCTGCCACGTTGATCTTGTTATGTACTAGACCGGTCAAAGTTGCTGCATTGGCGCATGCTCCATGGCTCTTGATTATACCTTCCATCATCATAATTGGTAGAGAGATAACCATGTCGGCACTCAGGGAATGGGCTGCTTCCCAGGGTAGTAAAACTTTAGAGGCTGTTGCGGTTAATAGTTTGGGGAAATGGAAAACAGCCACGCAAATGACAGCATTAACCATCCTTCTTGCTACCCGTGACTGCAGTCATGGAGGACCTGCGATTTTGGCAGGCTCTGGTGTTTTGTTGCTTTATATTGCGGCAGGGCTAGCCGTATGGTCGTTTGTAGTATATATGAGGAAAATATGGAAGGTGTTAGTGAGGTAACATGGACGACGACGGGTCTTGGTTTCTTTGATGGGTTTGGTTTTCACGAATCACGACCATTATTATTTCTTTCAAAAATAGTTTAGTCAGTGATACCGTGGAAGGGAGACGCAGTTCTCGATTCTCATGACATATATTTGTAATATTTACTATCTTCATTGTCTAACTCTGCAAACCACCATTGGTTCCATTTATGAAAATTATATGCACAATTTTTTTTGAAGTT
TARGET=90,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2364
PRIMER_LEFT_PENALTY=0.133741
PRIMER_RIGHT_PENALTY=0.102638
PRIMER_LEFT_SEQUENCE=TGACCTGACCTGATAGATGTCG
PRIMER_RIGHT_SEQUENCE=AAGCACGATCATTCTTGGAGAT
PRIMER_LEFT=36,22
PRIMER_RIGHT=318,22
PRIMER_LEFT_TM=60.134
PRIMER_RIGHT_TM=60.103
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.3119
PRIMER_LEFT_1_PENALTY=0.133741
PRIMER_RIGHT_1_PENALTY=0.178157
PRIMER_LEFT_1_SEQUENCE=TGACCTGACCTGATAGATGTCG
PRIMER_RIGHT_1_SEQUENCE=GTTGTCTTGCAAAATGCTAACG
PRIMER_LEFT_1=36,22
PRIMER_RIGHT_1=149,22
PRIMER_LEFT_1_TM=60.134
PRIMER_RIGHT_1_TM=59.822
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=0.3119
PRIMER_LEFT_2_PENALTY=0.133741
PRIMER_RIGHT_2_PENALTY=0.178157
PRIMER_LEFT_2_SEQUENCE=TGACCTGACCTGATAGATGTCG
PRIMER_RIGHT_2_SEQUENCE=CGTTGTCTTGCAAAATGCTAAC
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=150,22
PRIMER_LEFT_2_TM=60.134
PRIMER_RIGHT_2_TM=59.822
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=115
PRIMER_PAIR_PENALTY_3=0.9949
PRIMER_LEFT_3_PENALTY=0.892215
PRIMER_RIGHT_3_PENALTY=0.102638
PRIMER_LEFT_3_SEQUENCE=CCCTGACCTGACCTGATAGATG
PRIMER_RIGHT_3_SEQUENCE=AAGCACGATCATTCTTGGAGAT
PRIMER_LEFT_3=33,22
PRIMER_RIGHT_3=318,22
PRIMER_LEFT_3_TM=60.892
PRIMER_RIGHT_3_TM=60.103
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=1.0053
PRIMER_LEFT_4_PENALTY=0.902646
PRIMER_RIGHT_4_PENALTY=0.102638
PRIMER_LEFT_4_SEQUENCE=GACCTGACCTGATAGATGTCGG
PRIMER_RIGHT_4_SEQUENCE=AAGCACGATCATTCTTGGAGAT
PRIMER_LEFT_4=37,22
PRIMER_RIGHT_4=318,22
PRIMER_LEFT_4_TM=60.903
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_04225 Ps_contig_mira-and-tgicl-ass_franssen_32521
SEQUENCE=TTATTAATTCATCACACGCACCCACCTACTCTACTCTATNTTTAAATTTTTCTATAATTCCGTCTTGATTTGTTTTTTTGTTTGTTTGTGGTTGTTGTGTTTTGTTGGGTGGCGTGTGGAAGTGAAGAATAGTGATTGATATATAACAAAAAAATANGGCGTATCAATCGATTCAGGGTCCGAGTTCGGGATCGAGTTCGAGTTCAGGGTTTCAATTGTTGAATTCTCCGTTTGGAGATACCACTTATACTAAGGTATTTGTTGGGGGATTGGCGTGGGAGACGCAGAGCGAAACTATGCGCCGTTACTTTGATCAATTTGGGGATATTCTTGAAGCCGTTGTTATTACTGATAAGAATACAGGAAGATCTAAAGGCTATGGTTTTGTGACTTTCCGTGATCCAGAAGCTGCTAGGAGAGCCTGTGCTGATCCAAGTCCTGTTATTGATGGTAGAAGGGCTAACTGTAATTTGGCTTCGCTTGGTCGTCCGCGACCTCCTATCCCCTATGGACGGATAAGACCAGCATCCCCTTATGTTGGAAGTTTGCAACCAGCTCGTGGTGCTTACGTTGGAAGTTATGGCTACCAGCAACCACTTTCCTATGGCTATCAACAAGGAATGGTTTATCCTCCTTATGGGTATACGGCATATGGACCTGAATACGTCTACCCACAGAGTATGTACAACCCTTACGTGGGTCAACAATACCTTCAGATATATGGCGTGCCGGGTGGGGCGAACACAGCCATTTATCCTTATGGACAATTGGGTCAAGCTATTCCTGGCGGTCATGGTTATACAGCAATGCAGGGTTATACAGTACCAGCTCATCAGATTGTTCCCTATGGTGGATCCAATGTTAATGCAATGACAACTTCACCTGTGCCTGCCATTCAAGCCTCATATCCTAGCGGAATCGGTGCACCCGTTCCAGGCCAACAATTTATTGTTTCTCCTCAGTTCATACAAGGTAGCGGTCCTGACCAAACAGCTGGTTGAGGGGGCGATTAAGATATCCTTAGTTTGCAGCAGGACTAAGAGCAGTGGAACTGCTACTTGAGTGGCGGGCTTCTAATCTAGCCTTGCAAGTATTTATTTTGCATTCTAGAGGTCATATAGTCATGCTCACAAAGTTGTGTCTGAGTCAGCTCCAGATGGCGAAGGAAAATGGAACACGCAGGCTGCTTCTTCCATTTGGACGTTTACGCTACCGTATTTATACCTAATACTACTGGTACTCAAGAAGAGTGGCGGTAGTATTGGATCAATGGTTGTTAGAAAAGGTTTCTGGCAGCCATGTAGAAATAAAACTTGTGTAAAAAGAAAATCACGTCTGAATCAAATTATGATTCTCTTTAGTCCTCCTGTCCATTATTGGGTTTGTAGATAGTTATCATCGATGTTCATGTCGATGGATGGTCCATCACATCGAATATGACTTTTGTGCATAGTAGTTATCATAGGTTCGGTTCCACCACAAGACACGGTTGTAATTTTATGATTTTATGCAGAACCTGCCGTTAATGCAGGGTAGACCCATTAGAGAAGATTGCATTGTAGTGTTCATATTTTACCATCAGCATTCAGTCACATGTCTACTACCTCGTNATATTC
TARGET=77,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1733
PRIMER_LEFT_PENALTY=0.116923
PRIMER_RIGHT_PENALTY=0.056343
PRIMER_LEFT_SEQUENCE=ACACGCACCCACCTACTCTACT
PRIMER_RIGHT_SEQUENCE=CAATCCCCCAACAAATACCTTA
PRIMER_LEFT=13,22
PRIMER_RIGHT=271,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=59.944
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=0.3303
PRIMER_LEFT_1_PENALTY=0.273987
PRIMER_RIGHT_1_PENALTY=0.056343
PRIMER_LEFT_1_SEQUENCE=TATTAATTCATCACACGCACCC
PRIMER_RIGHT_1_SEQUENCE=CAATCCCCCAACAAATACCTTA
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=271,22
PRIMER_LEFT_1_TM=59.726
PRIMER_RIGHT_1_TM=59.944
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=0.5016
PRIMER_LEFT_2_PENALTY=0.116923
PRIMER_RIGHT_2_PENALTY=0.384647
PRIMER_LEFT_2_SEQUENCE=ACACGCACCCACCTACTCTACT
PRIMER_RIGHT_2_SEQUENCE=AACAATTGAAACCCTGAACTCG
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=219,22
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=60.385
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.6127
PRIMER_LEFT_3_PENALTY=0.116923
PRIMER_RIGHT_3_PENALTY=0.495821
PRIMER_LEFT_3_SEQUENCE=ACACGCACCCACCTACTCTACT
PRIMER_RIGHT_3_SEQUENCE=AATTGAAACCCTGAACTCGAAC
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=216,22
PRIMER_LEFT_3_TM=60.117
PRIMER_RIGHT_3_TM=59.504
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.6586
PRIMER_LEFT_4_PENALTY=0.273987
PRIMER_RIGHT_4_PENALTY=0.384647
PRIMER_LEFT_4_SEQUENCE=TATTAATTCATCACACGCACCC
PRIMER_RIGHT_4_SEQUENCE=AACAATTGAAACCCTGAACTCG
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=219,22
PRIMER_LEFT_4_TM=59.726
PRIMER_RIGHT_4_TM=60.385
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_04226 Ps_contig_mira-and-tgicl-ass_franssen_32523
SEQUENCE=GGCCGACACCCAAATAAACAAATTCACAATTCCACACCATCTTCCCCTTCTTTATCATCCAAAACAATGTATTCTATTACCAAATTTTCTCACCATAAAGACACCACCATGAACTCCACAAAAAGATACAACCAAATATCCCATTTCAGCCACACAAAACACAAACTAACATTCCAATACTCCGAATTCCCATTCAAATGTGATGGCTGCAAAGAAATTGGCATAGGCTCACGTTATAACTGCACCATTTGTGACTATGATCTTCACATGCATTGTTCCATACCATCTCCTTCTCTTTTCCATCCTTTCTACCCTAAATGTTGCTTCCAGTTCCTCTCAAAGCCACCCGGTGACATACCCCGTTACTGCAACGCCTGCGAAAAGAGTGTCAATGGTTTTGTATATCACTGTTTTTCCTGCGGTTTTGATCTTCATCCTTGTTGCGCAAAGCTTCCAATGATACTCGATGATCACGGTGAAATGAAGTTGTATCTATATCGGAAAGTGAGTTCGTCGTGTCAGCGTTGTGGACAAAAAGGGCGAAGCTGGAGTTACAGATCGTCGTGTAAGAAGTATAACCTGCATGTGGCGTGTGTGAGGGAGATGATTGTGGAGAGTTGGCATCAGATTTACGTTAGTCAGAGCAATGGAAGAGCAACAACAATGGTTGAGACAAGGGTTCCTCTGAGTTTGAAGAATGGTCTGCATACTGCACAAAATGGTGGGGGAAGAAGCAAAAGTAAGGTGAGGAAGTGTGCTGAGATGGCTGGAATGGCTGTACAGATTGTTGTGTCTGCTGTTTTAGGAGATCCAACGGTTCTTATTGCTGGGATTATGGGGTCATTGATGTCAAGAGCTTGATCTTGTGGTGGACAGATGTCTGGCATTGACAACAAATCTCACTTGACTCTACTTGAGAGTATAAAGACCTTTGAAAATTATGTTTAGAAAAAGTGATTATAATCATAAAGTATATAACAATGACAGTGAGTAGCACTCAAAGTATATATACCTTCAAGGCCTTGTCACTAAGTTTAATTTGATCTGAAAAATGATCTATGATATATATGAAAAATGCAAGAATTTAAGAAAGAAAGAAAGTGCTCTTTAGAGTTTTTAAAACACCAGTTTTTGCTGTAGGAAGACCTCTAATCAAGCAGTGTTTCTGTTAAGTTGTACACAGCTGTCAT
TARGET=1087,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1695
PRIMER_LEFT_PENALTY=0.117817
PRIMER_RIGHT_PENALTY=1.051675
PRIMER_LEFT_SEQUENCE=TCTGGCATTGACAACAAATCTC
PRIMER_RIGHT_SEQUENCE=CTTCCTACAGCAAAAACTGGTG
PRIMER_LEFT=879,22
PRIMER_RIGHT=1143,22
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=58.948
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=1.3043
PRIMER_LEFT_1_PENALTY=0.117817
PRIMER_RIGHT_1_PENALTY=1.186467
PRIMER_LEFT_1_SEQUENCE=TCTGGCATTGACAACAAATCTC
PRIMER_RIGHT_1_SEQUENCE=TTCCTACAGCAAAAACTGGTGT
PRIMER_LEFT_1=879,22
PRIMER_RIGHT_1=1142,22
PRIMER_LEFT_1_TM=60.118
PRIMER_RIGHT_1_TM=58.814
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=1.3777
PRIMER_LEFT_2_PENALTY=0.117817
PRIMER_RIGHT_2_PENALTY=1.259857
PRIMER_LEFT_2_SEQUENCE=TCTGGCATTGACAACAAATCTC
PRIMER_RIGHT_2_SEQUENCE=CTTCCTACAGCAAAAACTGGTGT
PRIMER_LEFT_2=879,22
PRIMER_RIGHT_2=1143,23
PRIMER_LEFT_2_TM=60.118
PRIMER_RIGHT_2_TM=59.740
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=1.4832
PRIMER_LEFT_3_PENALTY=0.431486
PRIMER_RIGHT_3_PENALTY=1.051675
PRIMER_LEFT_3_SEQUENCE=TCAAGAGCTTGATCTTGTGGTG
PRIMER_RIGHT_3_SEQUENCE=CTTCCTACAGCAAAAACTGGTG
PRIMER_LEFT_3=849,22
PRIMER_RIGHT_3=1143,22
PRIMER_LEFT_3_TM=60.431
PRIMER_RIGHT_3_TM=58.948
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=1.4832
PRIMER_LEFT_4_PENALTY=0.431486
PRIMER_RIGHT_4_PENALTY=1.051675
PRIMER_LEFT_4_SEQUENCE=TGTCAAGAGCTTGATCTTGTGG
PRIMER_RIGHT_4_SEQUENCE=CTTCCTACAGCAAAAACTGGTG
PRIMER_LEFT_4=847,22
PRIMER_RIGHT_4=1143,22
PRIMER_LEFT_4_TM=60.431
PRIMER_RIGHT_4_TM=58.948
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_04227 Ps_contig_mira-and-tgicl-ass_franssen_32527
SEQUENCE=GCGRGRGTCGATTAATGCTATCTCAATCAAAATCCAATATCCATTCCAAGTATGTCGGTTGAAGGCACATGTTAGAGTGGAAACAACGCGTAATGAAATTACAATCCCATCCTCTTCTTCTTCTTCTCTGAAACTCTGTTCCCATCAAACATTTCAAATCCATAAAACACCCAAATCAATTTCATGGCATAAAACCTAGAATCGATAAACAAAAACACTACCTTTAAGAAAATTAATATTTATAGAAAATCTCAAACCTGGCAAATCTTGGAGGCTTGAGGGCTCGTTCTTCGTTGAAGCAACCTTCCCTTGATTTGAACGACGACCACGAGGACTGTAGACCTTGGCGTCGTAGAATCACCATGGTTTTTTCCTGGGCTTCTGCCTTCAGGATCACACTTCTTCTTCTCCTTCTTGCTGCTGTTATTGCGGCGTGTTTCACTCTTCCTATTGAGAAGATGATGAAGGACTTCTTAATATGGGTTGATCGTGATCTTGGCCGATGGGGTCCACTTGTTTTGGCTGTTGCTTACATTCCATTGACTGTCCTGGCAGTTCCAGCTTCAGTTCTTACTCTTGGTGGTGGTTATCTTTTTGGGCTTCCAGTGGGCTTTGTTGCCGACTCTATTGGTGCAACTGTTGGTGCAGGAGCTGCATTTCTTCTCGGAAGAACGATTGGAAGACCATTTGTTGTTTCTAGGTTGAAGGATTATCCTCAATTTAAATCAGTGGCAACTGCGATACGGAGATCTGGATTTAAGATTGTATTACTGCTTCGGTTTGTTCCACTGCTACCATTTAACATGTTAAATTATCTCTTGTCGGTGACTCCTGTTTCACTTGTGGAATACATGCTGGCTTCCTGGTTAGGAATGATGCCAATAACACTGGCACTTGTATATGTCGGAACAACTCTTAAAGATCTTTCGGATGTAACACATGGTTGGAGCGAATTCTCCAAGACTCGTTGGGCATTTATCATTATCGGTCTCGTAGTATCGGTGGTTCTTATGATATGTGTTACTAAAGTTGCAAAGTCTGCTTTGGATAAAGCCTTGGCCGAAAACGAAGATATCGACATCGATGGGGTTACATCATCCTCGCCAGAATTACCAGTTGTTGCTGAACAAGCATCAGAGCTTAACCACCCACTCATAATTAAGATAGATTCTAATGAAGACAATCATGAAAAGTAAATTCATTTTTTGTGTAAATTCTTTCTTCCTCTCCTTATAGTTGATTTTTTTTTTCCTATTGATGTTCAAGAGATAGCTACCTACCAGGTCTTCATATTTGTTTGTGATTTGTACAGTTCCAACATTAGAAATATAGTCTGTGACAGGAGTTTTTAATCCTACCATCAAGATGACATTATTGACTATGAACAAAAAGTTCATGCTTGTTTATTTTRTACTTCAAG
TARGET=113,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04228 Ps_contig_mira-and-tgicl-ass_franssen_32557
SEQUENCE=ACCANTNAAAATGTTNCAGTTTTTAAATTTACTATAATAATAACATTGACTAACATTTCAATGGAATATTCTTCACCATAAAACAATGTATATGTATGTCCTCGCTTAATGCCCCATGACATCGGGCTTTTATAACCTAATCAAGAGACATACATCTGTTCCAATAAGCTAGGGAGACTGATAACTGCAGTGCCAGTGAAGTAATGCCCCCGACTAGAACCACCTTCCAACATGTTACCAATAGCAATATGACTGTCAAAGGAACAGAAATTAAAAAAAAACTGCAAAATGACTCTAGATCACTTCTTATCCTCCTCATTTGGTCGCTCCTCGTCATTCTTCTTCTGAGACTCAGACTGATTTTGCATGGAAGCCAGCAAATCTTTGACAGATGGATCATTTGGGTCAACCCCTGGAAGCGATGCAAGGATAGAAGATACAAAGGACTGGTCCGCCAACAATCTACCCACTGTGTCGTTGGGGGGGTCTGATACCGACAATTGGAAAGCTCTAGCCAACTCAGGGTCATCTGTAGCTGCTTCAGACATATCTGCATCTTTTACATCATGGCTAGCTGCAGGATCATCCATCGACATTGCAAGGGCCTGTTGTAGTAGAGCATTCTCATCATCCATCATGTCATCTGTCTTAGTCCCAACTTCAGAAGCTGCGGTACTGGTTTGCTCAGTCATAGTTGCATCCTGGGAAACAGCTTGCTGTTCACCACCTTTCTCCTGTTTGGAAGCATCCTCTGCAGCCTTTTTCGCAGCTGCTTCCTGCCTAGCTCTCTCCTCTTCCATTGAAACTCTTAGAGCAAGAGCCAATTCAGGGTCCAAGTTTGGATCAACACCAAAATCATATCCAGATACACCACCTGCAGATGCTGCTGCTGCAGCAGCTGCAAAACCACTTCCACCTTCTCCATCACCAGTAAATATAGGTGTACTTATAAGTACATCAGAAAGAGCATTTGGACCAGGTGGAACATGAACCATGTGGCTACTATCATTATTGTTTACCGCTGCAAGGAGTGCTTCAAGCTTCTCTGTCTTCCCCTCGTCATCTTCACCAAAGTTGACAATGTCAAGTGCAACACTATTCTTTTTCAACTTTCTCCCAATCATTTCCAACATTTTCTTCTCGTACTTGACAGGACTTCCAGAAAAGACAATAATCCTTTGCTGCTGCTTCTTATTCTGCCTATGCTTAAGAGCCAACTGTGCCACCTGAATGCCAGCGGCTAGGTTCATCTCACCACCTATTTCTAGACCATGCATACAAGCTAAGATTTTGCCCAAATCACTAGTAGGGGTAGCCAAAACCCGAACGCCTTTCCCCGCCATAGTGAGAACTCCAACAGTATTTTCAGGATTAGACTGAGTTTTAGCACCGCAAATGAGACTTACAGCGTCTGATTGGGCTTGAAATCGAGAAGGAGAGTAATCACCGTTACGCATCCATTCAGAGTTGTCAATACAGATCATCGTCGCCTCGAGCACCATTGTTGGAGGTTTGGAGAAGTTCGGAAGCAAGATTTGAAAGGATGATAGAAAAGTTTTTCTGAGAGAGAGGTGAGAGAAGAGAAAGAGAACTCAGGTACTTGCGAAGGAGGTTTTCTCCACTTGGGCTTTTTTATTGGAACGCCGAACACTATCATGG
TARGET=882,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8458
PRIMER_LEFT_PENALTY=0.659953
PRIMER_RIGHT_PENALTY=0.185822
PRIMER_LEFT_SEQUENCE=CATATCCAGATACACCACCTGC
PRIMER_RIGHT_SEQUENCE=GTTGGAAATGATTGGGAGAAAG
PRIMER_LEFT=856,22
PRIMER_RIGHT=1130,22
PRIMER_LEFT_TM=59.340
PRIMER_RIGHT_TM=59.814
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.8458
PRIMER_LEFT_1_PENALTY=0.659953
PRIMER_RIGHT_1_PENALTY=0.185822
PRIMER_LEFT_1_SEQUENCE=CATATCCAGATACACCACCTGC
PRIMER_RIGHT_1_SEQUENCE=GGAAATGATTGGGAGAAAGTTG
PRIMER_LEFT_1=856,22
PRIMER_RIGHT_1=1127,22
PRIMER_LEFT_1_TM=59.340
PRIMER_RIGHT_1_TM=59.814
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.8598
PRIMER_LEFT_2_PENALTY=0.485895
PRIMER_RIGHT_2_PENALTY=0.373881
PRIMER_LEFT_2_SEQUENCE=GCCTAGCTCTCTCCTCTTCCAT
PRIMER_RIGHT_2_SEQUENCE=CCTTGCAGCGGTAAACAATAAT
PRIMER_LEFT_2=778,22
PRIMER_RIGHT_2=1028,22
PRIMER_LEFT_2_TM=60.486
PRIMER_RIGHT_2_TM=60.374
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.8859
PRIMER_LEFT_3_PENALTY=0.659953
PRIMER_RIGHT_3_PENALTY=0.225996
PRIMER_LEFT_3_SEQUENCE=CATATCCAGATACACCACCTGC
PRIMER_RIGHT_3_SEQUENCE=CAACTTTGGTGAAGATGACGAG
PRIMER_LEFT_3=856,22
PRIMER_RIGHT_3=1076,22
PRIMER_LEFT_3_TM=59.340
PRIMER_RIGHT_3_TM=59.774
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.9781
PRIMER_LEFT_4_PENALTY=0.752117
PRIMER_RIGHT_4_PENALTY=0.225996
PRIMER_LEFT_4_SEQUENCE=TCTTAGAGCAAGAGCCAATTCA
PRIMER_RIGHT_4_SEQUENCE=CAACTTTGGTGAAGATGACGAG
PRIMER_LEFT_4=806,22
PRIMER_RIGHT_4=1076,22
PRIMER_LEFT_4_TM=59.248
PRIMER_RIGHT_4_TM=59.774
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_04229 Ps_contig_mira-and-tgicl-ass_franssen_32561
SEQUENCE=ACCTTTATAACATTATCATCTATTCCTTAGAAATTGTACAATTACCACATTATTATTATTAGTGACTCTAATTCAAGCCATGTCTCCTTCCATCCTTCTTTTCCTTTCTCTATTCGCAATTGCAGACTCGCAGTCCTTCGTCGGAGTTAACTACGGTCAGATCGCCAACAATCTACCAGCACCTGAAGTCTCCGCCAGGCTTCTCCAATCCACCACCATCGGTAAAGTTCGCATTTACGGCGCCGATCCTGCAATTATAAAATCTCTTGCTAATTCCGGCATCGGAATCGTCATCGGGACCTCAAACAGCGATATTCCAAGTCTAGCTTCCGATCAAAACGCGGCGAAACAGTGGGTAAACACAAACGTGTTGCCTTACTACCCTGCCAGTAACATCATTCTGATTACCGTCGGTAATGAGGTTTTAACCTCCGGTGATCAGGGACTCGTGTCACAGCTTGTGCCAGCAATTCGAAATGTCCAAACTGCCCTCAGCTCGGTGTCGCTTGGTGGAAAGGTGAAGGTATCCACCGTACATTCAATGGCGGTCTTGTCTCGGTCGGATCCACCTTCGTCCGGGTCGTTCAACCCGGTTTATCAAAACACACTCAATCAGTTACTAGCGTTTCTGAAGGATAACAAATCCCCGTTTGCGGTTAATCCGTATCCGTTTTTCGCTTACCAGAGTGACCCGAGACCCGAAACGCTTGCGTTCTGTTTATTTCGACCTAATTCGGGTCGGGTTGATTCGGGTAACGGAAAGCTTTATACCAACATGTTTGATGCTCAGGTTGATGCTGTGCATTCTGCTCTTAGTGCAATGAAGTATGATGACATTGAGATTGTGGTTGCTGAAACAGGATGGCCTTCTAGTGGGGACAGCCATGAAGCAGGACCAAGTGTAGAAAATGCCAAGGCCTATAATGGGAACCTTATTACTCATCTTAGATCATTAGTTGGTACACCTTTGATACCTGGAAAGTCCGTCGACACTTACATTTTCGCACTCTATGATGAAAACCTCAAATCTGGCCCGGGATCCGAACGCGCATTCGGCCTTTTTAAAACTGATCTTACCATGTCATATGATGTCGGCTTGGCCAAATCTAGCCAACAGAATCCGCCGCCAACTAGTCCAGTTACTCCAGCACCTAGCACTTCATGGTGTGTTCCAAAAGCAGGAGTTTCTGATGCTCAATTGCAGGCCAATATTGATTATGCATGCAGCCAGGGATTAGATTGTAGACCAATACAACCAGGAGGAGTATGTTTTGAACCAAACACATTACAATCCCATGCTGCTTTTGTAATGAATCTATACTACCAAATATTTGGTAGAAATCAATGGAACTGTGATTTCTCTCAAACAGCAACACTCACATCCCAAAATCCAAGTTACAATGGTTGCAATTATGTTGGTGGGAGTACCTGAAAAGCAGATGTTTCAGTTTGACCTTAGTTAATAAATTTTGTCTAAATAAACTATTAATTTTAATTTTAGTTTTAATCAACAAATAAGGGCTTATCCTCGGCCCTTTTTTATTTATAACCTTTTAATTTAGTATGACTTTAATTGTAAAGGCATTTCGATTCTGCCTTATTCTATTTTCTTCTTAATAGTATCTCCTTTGGTTTTGTAGCATGYTGCTCTAATAATGTGTCATTGGGTAATCCATTTTTTCATAGGTTAAATGGAAAATAAGTTATA
TARGET=49,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04230 Ps_contig_mira-and-tgicl-ass_franssen_32568
SEQUENCE=TACAACAGTGGCGGTGGAGGTGGTTATGGTGGAGGTGGTGGAAGATACAGTGGAGGAGGTGGTGGAGGCGGCGGTGGCGGTGGAAGCTGCTACAGCTGTGGAGAATCTGGTCATTTTGCGAGAGATTGCCCAACCAGTACCCGTTAGATCTCTGCGGATTACTTTTTATGAGTAATGTGTTGGTGATTTAGTTGGTTTTTTAAGTTTCAGAGTCGCCTTTTTGGTTTTGATTTTATCTTTATATTTTGCAATAGTTTTAGGGGTTTTTGTGCTGGATCTATCTATTATGCGAACTCTTGGTTTTTATAATGAATGAATGAAATGTCGGGCTTATTTTGTTA
TARGET=308,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04231 Ps_contig_mira-and-tgicl-ass_franssen_32571
SEQUENCE=TATCTAAATTCAGTGGACTTATTTTTTATTTATTTAATTATTTCATAACAGGGTAATAGTAGCATATAAATAAACAATTTTTTGATGCTTCTCTACTGGTCTACTTGTACTCCTGAGTCATATGTTTCTCCAGCCTTCCAGTCTTCTGGTATGTTATTAACAGGAACAACCCATGTATCGTCTCCATCTTCAGTACTAAACAACATCCTTAGAGACAAAGGTCCACTCGGTGGAGGGGTTGCAGTCCAGACTGCACCATGACTCCGATCCAGTAGCTTGCAGACAAAGTTCTGAGTCTCACAGAGCTGCACAGCTGTTATGTCTCTCCTTCCTTGTTGAAACCATATGACAAAAGCCAAGTAGTGAGGGTTGCTGCTGCTTTCATCAATCTTAATTGTGATGTTTTTGTTAGGGTAGCTACATGAAACCCTTCTGTATTCAATATCAACTACACCAAGAGCTAATAAAGAAGCGGATGCATCCTTGCTCTGAGCCATCCCACCAAAGGCTCGTTGGCTAAGAATGAAGTCAGTGTTATCGCCTGAGCCTTGGTCCGTTAAAACTATAGTGACACCGTTGCCCGAGCAATAAACACCGTTGGTACACCTTACCTGATAACAAGCGCCACAGCCAACTCCATCTCGATAAAGACTTGATGCAGCCGATACATCCCCTCCATTAATGGTAGCACCAAAGGAACCGAAAATACCACATGCACCAGCATCTGTGCCATTTTCTTCAGAGTTTGAGTAAAATGATGCACGAGATTGAACAAAACAATCGTTGCATGAGGTATCTGCCAAAGTTTGCATGAAAAGAAAGGTGACAAGGAGAACAAAACACAAAAAAGAGAGAGCCATGTAGGACAGAATGGATAAAGCAGTCAAAACTCAGGATGTTAAGGTAAAGCTTGTGATCCCCGACGTACTTANATTCTGTATTATAG
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04232 Ps_contig_mira-and-tgicl-ass_franssen_32574
SEQUENCE=AAAGAACAAGTAGGTAATACAAACGCAGAGTACGCGGGAAAACTCAAGATTCAATTCACACCCTAACAACAAAGATGAAACATCAAACATTCACATGCCTCTGTGTTCTGGCTCTAACCATCATAGTTTGTTCCAACACAGCAAACGGGGCAGAAGACTTGTCCAAAACATGTGCTCAGGTTGTTTCAAAGGTGATTCCTTGTTTGGATTTTGCGACGGGGAAAGCACCGACGCCGAAGAAGGAATGCTGTGATGCGGCGAAGAGTATAAAGGATACCTATCCGGAGTGTCTGTGTTACATCATTCAACAGACTCATAAGGGGAGTCCTGAAAGTAAGAGCATGGGGATTCAAGAGGATAAGCTTCTTCAACTTCCTGCTGTTTGTGATGTTAAAGCCAATATCTCTGATTGTCCTAAGCTTTTGGGGCTATCTCCGAGCTCCCCAGACGCAGCAATATTTAAAAACGTATCCAAGATCAATCCCCCTTCATCAGCTGCATCATCCACCGGAACTCCGAGCCCACCAACCCCGAGCGGTTCACATAAGCTTAGACCGGTCATGATCAGCGACATGATGATGATGACTGTAGCCTTTGTTCTTGCCGCAGTACCTACCGGATTTATCACCATTAAATTATAATGAGTTGGATCTTTAAATACTTCACCTAACATAAAGTGAAGTCTGGTTGGTTGTTTTGTTTACTGCATATCTGTTTTCTGTAGGACACTTAATTGGCTTGCATCTCTCTGCTCTGTGATGGTTTCTCGAATTGTGGGAACCTGCACTTTTATTTTACTGTTCTTATGTTTCCCATTAATATGGAGTACTAGTTTGGTCGTCATTATTTTCATAGTACTAGTACTATCTAAGATATAAGTATTTTCATAAGTTTCTCAAAAATATTCTTCTACTCAAAACATGTATTTTTATGCACTTTAAAGGTTTGATAAATTTAAAACCTT
TARGET=573,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1499
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.115779
PRIMER_LEFT_SEQUENCE=AAAGTAAGAGCATGGGGATTCA
PRIMER_RIGHT_SEQUENCE=GATAAATCCGGTAGGTACTGCG
PRIMER_LEFT=330,22
PRIMER_RIGHT=625,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.884
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=0.1499
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.115779
PRIMER_LEFT_1_SEQUENCE=TGAAAGTAAGAGCATGGGGATT
PRIMER_RIGHT_1_SEQUENCE=GATAAATCCGGTAGGTACTGCG
PRIMER_LEFT_1=328,22
PRIMER_RIGHT_1=625,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.884
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.1499
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.115779
PRIMER_LEFT_2_SEQUENCE=AAGTAAGAGCATGGGGATTCAA
PRIMER_RIGHT_2_SEQUENCE=GATAAATCCGGTAGGTACTGCG
PRIMER_LEFT_2=331,22
PRIMER_RIGHT_2=625,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.884
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.1645
PRIMER_LEFT_3_PENALTY=0.048692
PRIMER_RIGHT_3_PENALTY=0.115779
PRIMER_LEFT_3_SEQUENCE=CTTCAACTTCCTGCTGTTTGTG
PRIMER_RIGHT_3_SEQUENCE=GATAAATCCGGTAGGTACTGCG
PRIMER_LEFT_3=365,22
PRIMER_RIGHT_3=625,22
PRIMER_LEFT_3_TM=59.951
PRIMER_RIGHT_3_TM=59.884
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.1674
PRIMER_LEFT_4_PENALTY=0.122257
PRIMER_RIGHT_4_PENALTY=0.045112
PRIMER_LEFT_4_SEQUENCE=CTGATTGTCCTAAGCTTTTGGG
PRIMER_RIGHT_4_SEQUENCE=CAAAACAACCAACCAGACTTCA
PRIMER_LEFT_4=405,22
PRIMER_RIGHT_4=698,22
PRIMER_LEFT_4_TM=60.122
PRIMER_RIGHT_4_TM=60.045
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_04233 Ps_contig_mira-and-tgicl-ass_franssen_32580
SEQUENCE=CTAGGTAAGAGTTCAACCACAATACCATGTAACATACCATGTACGTGTCATTGTCAACAACTCAAATGGATCACATTAACTATCAACAATTCAACATAAAATAAAAATGCAAGATTCACTACTCATAAATAAATAAATATACATGAATAGGTTTAAATTACACACACAATGAAAATAGCTCTAATGGTAACAATGCTCACAAAAAACTTCAAAACTCGAAATAATCCCTCATGTCATAACCTTCCTACTCTCAATGAAACTGAACATGGCTACTCAAATGCTTGAGAATAGAGATAAGAGACAAACAAGAATGGACCCTGCTGAAATAGGGAGCAATGCTGATGCTGCCGCGCTCTTGACGCCCGGCGCGGCTGCCGGTACTCTAGATGGAAGCACAGGAGTGGCCACACCTGGTGCTTCAGCAGTAGGAGAAGATGCTGGAGAGAGACTTACACCAGGAGCAGCAGCAGGGGTTACAGACAATCCACAGTTAGAGAGAGAAGGAGCAGAGACTTTGCAAGCAGAAGGAAGAGTCAGCGCCTTTGAAACATTCAAAACAACGCCAAACTGAGCACTGCTTTTGAAAGCTTCACAAAGACAAGAAGGAGCACTTTTCAAGACAGTTTTCAAGCCAGAACAGCATGTTCCTTCTGGTTTGGTTGTGGTACTGTCATTTGTAACAAATGACAAACAATCAGCCATGGTTAGAACAAGAGTTGAACAATCAACAGAGGGTGATGGAGCATGGTGTGATGATGAAGCTGCGTTGGTGAAATGAATGGCCCAGATGGTGAAAATGCAGAGAATGAGTGAAAACTTTGGTGCCATGGTGAAGAGGGAATGGATAAAGGAATGAGTAATGGGGAGTTAGTTAAATATAGGGATGTAGTGAAGATTGATGAGGGTTTTTTGTGTGTTATTTTGTTTTTTTAGTTTGAATGGAAAATGCAAGTTTTGAAAAGTTTTGTTTTTGAAATTTGAATGGAAATTGCAAAGTTTTGAAAAATTTTGCAAGAAAAAGAGGATTTTGATGAAGAGGATGAATCTGTGTATCTGTTTGTGTGATCCTGATGTGTAACAGTCTATGGTTATTTATGTGTAATAAGAAGAAAATARGNTCGTT
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04234 Ps_contig_mira-and-tgicl-ass_franssen_32590
SEQUENCE=AAAAACAAAACTCAACANATTTATGAAGTGCCATCTAATATTTATGTATAAAAAACAAAACTAGCAAAGTGCAAGGATAAACAAACCAGTACATATCCGGAGAGACTAAAATTTTGCATTAATCAGTCAGAGCCTAAAACAAAGTACAAACAGCTTAATACTAGAAAAAAATAAACTGAACCTAAAATGCACCTACAGAGTCCATACATATTCTGGTGCATCTTCCCTTTTACCAGCCACAGAACGATACAAATACATCTTCTCCACTCTTTGCCCATCTTCCACATAACAATCTGCTTGTTCACTTTCATTGAAGATCTCCACATTCAAACTAGGCATCTTCTTCGCCAATGTCTTGCATGCTCCTACAGTCACCTCACACGACGACATCCAAAGGGATCGCATTGTTTCATACTTCCCTACGTCTGTCAGAAGTGCTGTGTCGCCGAAAGGGCAGTCTCTTATCTCGAGCTTGCGAAGCTTTTTGCAACCGTTCAATACATAGAGCATTCCCTTGTCACTCTCGCCAGCAAAAGCAATAGATAACATTTCAAGCTGCTCCGCGTACATTCCAATGTAAAGGAAGACCTGGTCGGTCAACTGACCGGAGAGTGATAGCCGCCTCAGTCGTTTGCATGACTGTACGATTGCCCCAAAACCTTCATCCAGTGGCTGCATTGTGTCGGAGTCAGGTTTTGTTGCATCGAGGATGCATAACCTAAATCGGATAAAATTTGGACAGTTCTTGGCTACTGTTATGAGAGCAGCATTTGTCATCTGGTGGCAGAAGTAGAGTAACGAGTGGAGCTTTGGGCATCCCTTTGATATCGCAACAAGTCCTACTTCGGTAACAGCTGCTTGATTTCCAAATGGTGCGGATGGAAATACCCTCAATTCTTGCAACTCTTTACATATAGTAGCTACAGCAACTAGTCCTTTGTCTCCAATGCAATCCATTATCCATAAGCGCTGTAGTTTGCCGCAATGGCGAATAAGTTTAATAAGCTCAGCGCCCTGAATGCTTGCTGCATAGCTCAAGTTCAAGGATGTTAAGTTCCGGCAAATTGGATACACAGCAGCAAGGCTAAAAGGAGCCACTTCCAAAAATCCGGACAAACTCGTTATTGACCGGCATCTAAGAATGGTATTCTTAAGCTTTATGTAGGCCTCTGAATTGAGATCATGGATAAATGATCCAATACCCAAATCTGCTATTTGAGGCGCTCGCATTAGTATCCTTTGAAGTGCATCAGCCGGCACGGAACGGTTTAACCTTAGACTCTTGAGGTTAGGTGATCTTGCCACAAGTCTCTCAAGTGCTCCCACGTTAATCTCTCCTTTAAGGCAAGCAAAATTAAGAGCAACGAGTGATGTACAGTTTTCCGGAAAACAACTTAGCCACTGTCCTTTGTGATCTTCAACTTCATTCTCTTGCAAATCTAGCTCCCTAAGAGACCTGCAATTTGCAGCTACAGCAGCAAGTCCATCAGTGGTGAACCCTTCACAGCTAACAAGAACTAAAGACTTAAAATTCATGAAAGAACGAGACAGTAGCTCCAGGCTCTCATCTGACACAACCATCCTCTTCAACCTAAGCTCCTCCAACCCAACTCTACTCTTAGCAAGAGCTTCAATCCATGGATAAACAAAACCACCCCAACCATGAGGAACCAAACTGAAGTCAGCAAAATGAGGTTTTCCCTTTAGAGTTAAAGATTTGAAATCAGGAAACCTCTCTACCAACCTCTCAGGACTAATAGAGTAACAGTTTCCTATGAACACCCTTTTCCTTGTAAATCCCTCTATTCTATACCAACTTTTGCATACCAAAGACAAACTGTTTCTGTCGCTATGTGACACCACATAGTCAAACACATGTTCTATTACCTCGTCTAGGAAAATAATTCATTTCTCTCTCTCTCA
TARGET=1911,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04235 Ps_contig_mira-and-tgicl-ass_franssen_32593
SEQUENCE=CGGGGACTAYTNRNNGGAAAGTAGGGTTTAATTTTCCGATTGAAATTTTGTGTAGCGCTACCTATCTCTCACTCTCTCCGCCTTCCTGCAAATCGCATTTGCCTGAAACTAAACTCCGCCTCTTAGAAACCAAGCAAGAAAAATGTCAGGTGAAGAAGAGGTTGTTGCCGCAGTCGAGCCTGTTGCTGCTGCTGCTGTAATTCCCGGTGAGCCAATGGACATCATGACTGCCCTACAACTTGTGCTTAGAAAGTCGCGGGCTTATGGCGGTCTTGCACGAGGTCTTCACGAGGGTGCTAAAGTGATTGAGAAGCACACTGCACAGCTCTGTGTTCTAGCCGAAGATTGCGACCAACCTGATTACGTCAAATTGGTGAAAGCCCTTTGTGCAGAGCATAATGTTAGTTTGTTGACAGTTCCAAGTGCAAAGACCCTAGGAGAATGGGCTGGTTTATGTAAGATTGATTCTGAAGGAAAAGCTAGGAAGGTTACTGGTTGCTCTTGTGTAGTTGTCAAGGATTTTGGAGAGGAACACGAAGCCTATAATGTTGTTTTGCAGCATGTCAAAGCTAACTGAGTTGCAATATGCATTTGAATTTATGGTTATCAATTTGAAGTTTTTTGCAAGAGTCTAGTGAATTTCACCTCTGTGGAGGATTTTGATGGATTATGGTTTAAAATTTTATTTATCTCATAGCTGCTCTTACTCAATTGACTATACTGTGATTGTCTCTGTTACTGAGATTGACCTGAGTGAAATGTTTATGACTAATCAAAGTTCGAACCCAGTAACCCGATTGTGTTAATCTCAATCACTCATTTTGTTCGAACC
TARGET=184,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04236 Ps_contig_mira-and-tgicl-ass_franssen_32594
SEQUENCE=AACGCAGAGTACGGGGACACAAGTAAAATCCCCATCTTTTCTCCACTCCTCCGTCGAACTCACAACTGTAAACCTTGGAGTAATAGAGAACGACGAAACCACCGAGTTCAAAACTCGCCGTGAGAACGATTCGAAACGAGTCACAACCCTAGCATGGATCCTCTGGAAGCAATAGTTGCTCAAATCCAAGGGCTATCGAGTTCCTCCTCCGACATCTCTCGCCTTCACACAATCTTGAGACAGTCCGATGACTCGCTCCGTTCTGAATCAAATCGTTTGTCTCCGATTCTCTCTCTCATTGATCCTTCCATTCATTCCCTCGGTTTTCTCTATATCCTAGATGCATTCACAGCTTCTTCGGCTTTGAATCAGCCGCAAGCTGAGGAGATAGTTCCGATCATAACAAGATTTATCAGTGCTTGCAGCGTGGAGCAGATTCGGCTGGCGCCAGAAAAATTTGTATCTGTATGCAAGAGGTTGAAGGACCAAGTAATGCTGCTGGAAGCTCCCATACGCGGGGTGGCTCCTTTGTTAACTGCTCTTCGGAAACTTCAAGTCTCCACTGAGCATTTGACTCCCCTGCATGCAGAGTTTCTTCTGATTTGCTTGTTGGCAAAGTGCTACAAAACTGGTCTATCCATATTGGATGATGATGTTTTTGAAGTAGACCATCCGCGAGACCTTTATCTCTACTGTTATTACGGGGGTATGATATGCATTGGACAGAAGCGCTTTCAGAAAGCATTGGACCTTCTGCATAATGTTGTAACTGCTCCCATGACTATGATGAATGCTATAGTTATTGAAGCTTACAAAAAATATATATTGGTTTCTCTTATTCGTCATGGACAATTCTCTACCAGTCTTCCCAAGTATTGCTCTCCACTTGCCCAAAGAAATCTGAAAATCTTTTGTCAGAACCCTTACATGGAAATAGCACATACTTATAACACTGGAAAAGTTGCAGACTTAGAGGCATTTGTCAATGCAAATGCAATCAAGTTCGAATCTGAGAACAATCTTGGACTGGCTAAGCAGGTTGTATCATCTATGTATAAGCGGAATATTCAGAGATTGACACAGACATACTTGACCCTTTCGCTCGAAGACATAGCCAACACAGTGCATTTAAATAGTGCCAAGGAAGCTGAATTGCATGTCCTACAAATGATTCAGGACGGGGACATATATGCAACAATCAACCAGAAGGATGGAATGGTGAGGTTCTTAGAGGATCCGGAGCAATACAAAACCTGTGAAATGATTGAGCATATTGATTCCTCAATCCAGAGAATAATGGCACTGTCGAAGAAACTTACCAAGTCGGACGAACAAATTGCATGTGATCAGTTGTACTTGTCCAAGGTTGGAAGAGAGAGACAGAGATACGACTTTGATGACTATGATGTTCCACAAAAATTCAACATTTAAAGCTAGGCTACCTGTTCATAAGAGAGCTTGTTTGGTGCTGTGAATGATGCCAAGTAGATCTTGTTTCTATTTCGTTACCCTTTTTCCTTTTAACTATTTACTTATCGTTGGGTTCTTAGTTTAAGTTGGGGTATTTGTTTTTATTTTCGCCTACCAAATGGTTA
TARGET=288,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1291
PRIMER_LEFT_PENALTY=0.060824
PRIMER_RIGHT_PENALTY=0.068302
PRIMER_LEFT_SEQUENCE=AAACGAGTCACAACCCTAGCAT
PRIMER_RIGHT_SEQUENCE=TAGAGAAAACCGAGGGAATGAA
PRIMER_LEFT=133,22
PRIMER_RIGHT=331,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=60.068
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.1639
PRIMER_LEFT_1_PENALTY=0.095574
PRIMER_RIGHT_1_PENALTY=0.068302
PRIMER_LEFT_1_SEQUENCE=TGGATCCTCTGGAAGCAATAGT
PRIMER_RIGHT_1_SEQUENCE=TAGAGAAAACCGAGGGAATGAA
PRIMER_LEFT_1=154,22
PRIMER_RIGHT_1=331,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.068
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.3000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.2356
PRIMER_LEFT_2_PENALTY=0.231272
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=ACACAATCTTGAGACAGTCCGA
PRIMER_RIGHT_2_SEQUENCE=GGTCCTTCAACCTCTTGCATAC
PRIMER_LEFT_2=226,22
PRIMER_RIGHT_2=486,22
PRIMER_LEFT_2_TM=59.769
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.2716
PRIMER_LEFT_3_PENALTY=0.203343
PRIMER_RIGHT_3_PENALTY=0.068302
PRIMER_LEFT_3_SEQUENCE=CCGTCGAACTCACAACTGTAAA
PRIMER_RIGHT_3_SEQUENCE=TAGAGAAAACCGAGGGAATGAA
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=331,22
PRIMER_LEFT_3_TM=60.203
PRIMER_RIGHT_3_TM=60.068
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=0.2801
PRIMER_LEFT_4_PENALTY=0.275745
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=ATCTCTCGCCTTCACACAATCT
PRIMER_RIGHT_4_SEQUENCE=GGTCCTTCAACCTCTTGCATAC
PRIMER_LEFT_4=213,22
PRIMER_RIGHT_4=486,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_04237 Ps_contig_mira-and-tgicl-ass_franssen_32600
SEQUENCE=GGNGTAATAGGAACGTAAGGTGTTTACGAACTCTTATTTACTTTAAAACCAGTATCTTACTTGAAGAAGGTGACAAACATCTATAACAAGCCCTGGTCATTACTGCTATATTCTCTGTACAAAAATATAAGCTAACTTGCCTTGTTAACATATGTCAACAGGCCTCAAACACACAAGTTTGGAAAGTACTCAACTTTCGCTGACAACCTTTCGGATAATATAGACCTAAAATTGCTACAAACGGCAAAAACAGGGATCTAATACCTAGATCAATGTTATCTCTATCCCTAAAATTGATGCAGTGGTAAACAAATGTAAGGAAATAAACAATTTGGGAAAAGAGTATTAAGCTCTTGGATAGCGTAAGCGCCCTTTCTTCTTTAAGAATTCTGGGATCTCAACCAAACTGCCATCAGTGAAAGTGGAAGCTCGCCGACTGACCATGGTATCTCCCTGTGTGAATTGACTGGCCTGTCCGGGTCTCCCTTCACTTTCCTCTTGACGCTTAAATCCCGTCGCAATCAATGTTATGCTCACTTGACCACTGAGCGATGGATCTATTACTGCTCCGAATATTAAATTAGCCGTAGGGTCCACAAGATCATATATGACTTCTGCTGCAGCATTTACCTCAAACAATGTCAGATCACTTCCACCAGTTATGTTCCACACAATTCCAGTGGCTCTCTCAATACCAATATCTAGTAAGGGCGACTGGATAGCATTTAATGCAGCATCTCTTGCCCGTGTTTTCCCAGTTGCAGTTCCTATCCCCATAAGTGAAGAACCTGCATTGGCCATTATAGCTCTAACATCGGCAAAGTCAACATTCACCAATCCAGGAATCGTTATAATATCAGAAATGCCACGAACACCTTGTCGAAGAATATCATCAGCCAGATTGAATGCTTCAGTTACAGGGGTAGACTGAGAAACTGCAGTCAGCAACTTATCATTTGGAATAACTATCAGGGTATCAACATTATCTCTTAAGGCTGCAATTCCTTCTTGTGCTTGAACTGCCCTTCTCCGTCCTTCAAATGAGAAAGGGGTTGTGACAATACCAACAGTTAGTATACCCATTGACTTTGTGATACCAGCAATAACTGGAGCTGCACCAGTGCCAGTTCCTCCACCCATTCCAGCAGTAACAAAGACCATGTCAGCTCCATAAACCGCCTCTTGTATTGATTCTTTGCTTTCTTTGGCAGCATTCATACCAATCTCCGGGTTACCACCAGCACCAAGACCCCGTGTAAGCTCTTGACCAATTGGCAATCGGTTCTCGGAATTAACAGGCGACATTCTCATAGCCTGAACATCAGTATTAACAATCCAAAACTCCACACCATTCATGGAACTCTCAATCATCCGGTTAACCGCATTCGACCCACCACCACCAACACCAATAACTTTAATCTTCGCTTCATTATAATTACTTGGAATGGAAATGTCTTCTAAGCTCTCCGCCACATCTCCACCCGACGTATCCTTTCTCTGTCTAGCAGGGTTGCTTCCTTCACCTCTAAGCATTGAAACTTCGGGATGAAGATCTAAAAAAGGATCTTTGCTATGATGATAGTGACCGATACTATGGGAATTCGGTAAACCAGTCGATTTCATTTGAAACGAACCGCACTTTCTGTTACCGCTAAAAAATCGACACTTATTCTCATGAATTTTGAAGAAACAGGGTTTGTTAATGTGAATGTGATGATTCTCTAACAACTTTCTTCCTCCATTAACAGCCAACACTCCTGCTGAATTTCTAGAATTCGAAGGTGCAAAACACGTTGCCATTCTTACAGTATCCAAACTCATAAGTTCTAATTAATGTACCAAATTAGGAAAGAAAAAAAATTACATAAGTAAAAATTAGCAATTACGGTTTACCTAAATTGTCCTTTCCTTTACCTTAAACCG
TARGET=1391,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1022
PRIMER_LEFT_PENALTY=0.027712
PRIMER_RIGHT_PENALTY=0.074466
PRIMER_LEFT_SEQUENCE=GGAATTAACAGGCGACATTCTC
PRIMER_RIGHT_SEQUENCE=AGATCTTCATCCCGAAGTTTCA
PRIMER_LEFT=1287,22
PRIMER_RIGHT=1554,22
PRIMER_LEFT_TM=59.972
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=0.1419
PRIMER_LEFT_1_PENALTY=0.067436
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=CCATTCATGGAACTCTCAATCA
PRIMER_RIGHT_1_SEQUENCE=AGATCTTCATCCCGAAGTTTCA
PRIMER_LEFT_1=1348,22
PRIMER_RIGHT_1=1554,22
PRIMER_LEFT_1_TM=59.933
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.1700
PRIMER_LEFT_2_PENALTY=0.095574
PRIMER_RIGHT_2_PENALTY=0.074466
PRIMER_LEFT_2_SEQUENCE=AATCCAAAACTCCACACCATTC
PRIMER_RIGHT_2_SEQUENCE=AGATCTTCATCCCGAAGTTTCA
PRIMER_LEFT_2=1332,22
PRIMER_RIGHT_2=1554,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.2547
PRIMER_LEFT_3_PENALTY=0.027712
PRIMER_RIGHT_3_PENALTY=0.226971
PRIMER_LEFT_3_SEQUENCE=GGAATTAACAGGCGACATTCTC
PRIMER_RIGHT_3_SEQUENCE=AGACAGAGAAAGGATACGTCGG
PRIMER_LEFT_3=1287,22
PRIMER_RIGHT_3=1502,22
PRIMER_LEFT_3_TM=59.972
PRIMER_RIGHT_3_TM=59.773
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=216
PRIMER_PAIR_PENALTY_4=0.2769
PRIMER_LEFT_4_PENALTY=0.027712
PRIMER_RIGHT_4_PENALTY=0.249232
PRIMER_LEFT_4_SEQUENCE=GGAATTAACAGGCGACATTCTC
PRIMER_RIGHT_4_SEQUENCE=CCGAAGTTTCAATGCTTAGAGG
PRIMER_LEFT_4=1287,22
PRIMER_RIGHT_4=1543,22
PRIMER_LEFT_4_TM=59.972
PRIMER_RIGHT_4_TM=60.249
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_04238 Ps_contig_mira-and-tgicl-ass_franssen_32606
SEQUENCE=CGTAATAACTATACCAAAAAGTATTCGAATTTCGAAGTTGGAGCCAAAGAGGTGGATTGGTCCTTATCACTATGACACTGTCGAATGTCGAGATAATAAGGTGACCCCTCTGTGTCCTTTTGATTTTAACACCATTGGAGTAAAGTTTATAGATCTAAAGCTTACTACAAACACACATTAAAAACACATAAAACGAACCACACAACACAACCATGAACCATCTCCATACTAGTACTACACTACAGAAAGTAAATTACAAAGACAAAAAAAAACATAGATTCTCTTTTCCTGATTTTATTATTTCCCTCAACCACGTATCAGGAGTAGGTCACTAAACAATAACCCAACCTAGACACATTTTGTCATAAACATTTAGTGGCAAAAGATAACCTTAAGCTCACGAGGGGAAGAACACTGATGAGTCAAAGAAGGTGTATCATGCGCATAGGTAAAAGTCTCAGGACACGCGTGCTTGAAGAAATTCGTATACACGGTAGGTTTACAAGTTTGAGGGTTGTTAAAATGGTTCCTACAACAGAACTCATCTGTGTGAAAAGCTTCACATCCACTCTTACAGGCCACAACAGGACCATGAACAGCAGGAACACGGTGCTGCAAAACATCAGGACACGTGGCAACGAGATTTGCTTTACAACCAACCACGGGGCACTGACCTTTACCCTCGTGCGGTGTAACTGTCAGTGGGGTGTTGAAACCGTCGACGAGGCTGACGCTGTAAGAGGAGAAGTCGTTGTTGCCGTGGTGTACGTCGAACTGTACCAGTGTGGCGGGTAGTGAACCGCCTGCTCCGTTGCATTCGAGACGGTTGGCGCAGTCGCCGGTGAGGCAGGTGAATTTGTTGTTGGACGTGGAGCAGCCGGTTCTGGCCCAGGCACGGCCGGCCCAGTGGGTGTCTGGAACGTTGATGGAGCGGTGGGTGAAGGTACGGAGCTCGAAGCCGCCGCTGGCGAGGTTGGTGTGGCCTTCGTTGGGGAGAATAGCTGGCCAGACAGTGTAGGGGCAGTTGTTAACGAAGGTGAAGAAAAGAGCGTGGGAGGATGAGAAAATGGTGAGGAAGAGGAAGGTGGAGATGAGAAGCGAATTGGTTAAGGAGGACGAAGAAGAAGAAGCCATTGGAGCTGTTACTGTATTTGTGAATTTGTCCCCGACTTAGAA
TARGET=1118,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4123
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.352395
PRIMER_LEFT_SEQUENCE=GGATGAGAAAATGGTGAGGAAG
PRIMER_RIGHT_SEQUENCE=TCTAAGTCGGGGACAAATTCAC
PRIMER_LEFT=1056,22
PRIMER_RIGHT=1174,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=60.352
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.4123
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=0.352395
PRIMER_LEFT_1_SEQUENCE=AGGATGAGAAAATGGTGAGGAA
PRIMER_RIGHT_1_SEQUENCE=TCTAAGTCGGGGACAAATTCAC
PRIMER_LEFT_1=1055,22
PRIMER_RIGHT_1=1174,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=60.352
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=0.4480
PRIMER_LEFT_2_PENALTY=0.095629
PRIMER_RIGHT_2_PENALTY=0.352395
PRIMER_LEFT_2_SEQUENCE=AGACAGTGTAGGGGCAGTTGTT
PRIMER_RIGHT_2_SEQUENCE=TCTAAGTCGGGGACAAATTCAC
PRIMER_LEFT_2=1007,22
PRIMER_RIGHT_2=1174,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.352
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.5351
PRIMER_LEFT_3_PENALTY=0.182682
PRIMER_RIGHT_3_PENALTY=0.352395
PRIMER_LEFT_3_SEQUENCE=GAAGAGGAAGGTGGAGATGAGA
PRIMER_RIGHT_3_SEQUENCE=TCTAAGTCGGGGACAAATTCAC
PRIMER_LEFT_3=1074,22
PRIMER_RIGHT_3=1174,22
PRIMER_LEFT_3_TM=59.817
PRIMER_RIGHT_3_TM=60.352
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=0.6618
PRIMER_LEFT_4_PENALTY=0.309418
PRIMER_RIGHT_4_PENALTY=0.352395
PRIMER_LEFT_4_SEQUENCE=GAGAAAATGGTGAGGAAGAGGA
PRIMER_RIGHT_4_SEQUENCE=TCTAAGTCGGGGACAAATTCAC
PRIMER_LEFT_4=1060,22
PRIMER_RIGHT_4=1174,22
PRIMER_LEFT_4_TM=59.691
PRIMER_RIGHT_4_TM=60.352
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_04239 Ps_contig_mira-and-tgicl-ass_franssen_32610
SEQUENCE=ACCCGGGGTACGACCATGGCGGAATATCATGTGGGAAATTTGACGGGGTTGTGCAAACACTGGYTTTTGCGAATTTCAGCGAGGAATTGATTGTACGTAGTGCAGGGCCTGCGGATGGGGAGGTTATCCATCTGACTGTGGATTACAGCAAGAAACCACTGACAGTTGATCCAGTTTTCCGGCGACAAGTTGGTCAACGGCTTTGTGCAGTTGGGTTTTTCCTGGAGAGAAAGTTTGGTAGCCCACAGGATGTGGAAGGATGTCTTGTTGGAGAAGAGATTTATATTGTTCAGACAAGGCCCCAACCTCACTAGAAGAAATATGGAGAACAAAGTCGGACACAATTTTGAATATCAAAGCCTGTATACCATTGTATCATACTTGTTTAAAAATAAATCCTTTGTAATAATAATAATAATATAATATGTGTGACTTGTTCATGCAATAAATATTTGAAGAGA
TARGET=404,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04240 Ps_contig_mira-and-tgicl-ass_franssen_32616
SEQUENCE=TCGGGGAGAGACGGAGGCAGAGGCACAAACAAAAAAACGTTCTAAACCCCAAATAAACCCTTTCATCACCGCCGCCGCCGTCTCTCTCTCTGCACCGTCGCCTTGCGTCAGAAAGATATTGCAGCTGTATCACGGTGCGAGATGGAGAGAACTCACGACAGTGAAGCTCTTCCAAACAGCAAGCCTTCCACCGAACAGGACATTATTGATGAGCTAAAATTGAGATCAAAGAAGTATCTCAGAGGAGAAGGTGGTAATTTGAAGGGTTTAAAGGATAAAAAGACTTAGGAGTCAGTTAGTTGCTAGAGAGAAATTATATGGAGAATCTGCGAAAGCTGCTGCCAAGGCTGAGAAATGGATTTTGCCTAGTGATCAAGGTTTTTTTGGAGGCTGAAGGCATAGAAAAGACATATAGGATTAAGCAGGAGACAATAGCCCGTGAAGTCGATATTTTAAGCTCAAGGAACCAATATGATATTACTTTACCAGAGCTTGGTCCATATACTCTAGACTTTACTTCAAGTGGCCGGTATATGGCTGTTGGCGGTCGTAAGGGCCACCTAGCAATTGTGGACATGATGAATCTGAGCCTGATCAGAGAGCTTCAGGTAAGAGAAACTGTGCGTGATGTGGTATTCTTACATAATGAGCTCTACTTTGCTGCTGCCCAGAAAAAGTATTCATACATATATAATAATGATGGCACAGAGCTTCACTGCCTAAAGGAACATGGTTCAGTGTTGAGGCTGCAGTTTCTGAAACACCATCTTCTCCTGGCTTCTACCAACAAATTTGGGATGCTTCTTTATCAGGATGTTACACTGGGTGATTTAGCTGGGAAATATCGAACTGGTTTAGGACGTACAAGTGTGATGGAAGTAAACCCTTACAATTCGGTTGTTTCTTTAGGTCACTCAGCCGGTACAGTTACTATGTGGAAGCCAACTAGTTCTTCTCCCCTGGTCAAGATGCTATGTCATCACGGGCCAGTCTCAGCATTGGCATTTCACCCAAATGGCCACACAATGGCCACAGCTGGTAAAGATAAAAAATTAAACTTTGGGACTTGAGGAAGTATGAGGCTTTTCAAACATTACCAGGCCATGACAAAATAAGTTTAGGAAAGTTTAGTCAAAAAGGCTTGCTTGCTTGTGGTA
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4606
PRIMER_LEFT_PENALTY=0.283555
PRIMER_RIGHT_PENALTY=0.177008
PRIMER_LEFT_SEQUENCE=ACCCCAAATAAACCCTTTCATC
PRIMER_RIGHT_SEQUENCE=TCATCAATAATGTCCTGTTCGG
PRIMER_LEFT=45,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=60.284
PRIMER_RIGHT_TM=59.823
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.5890
PRIMER_LEFT_1_PENALTY=0.412035
PRIMER_RIGHT_1_PENALTY=0.177008
PRIMER_LEFT_1_SEQUENCE=CCCAAATAAACCCTTTCATCAC
PRIMER_RIGHT_1_SEQUENCE=TCATCAATAATGTCCTGTTCGG
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=59.588
PRIMER_RIGHT_1_TM=59.823
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=0.6536
PRIMER_LEFT_2_PENALTY=0.412035
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=CCCAAATAAACCCTTTCATCAC
PRIMER_RIGHT_2_SEQUENCE=ATAATGTCCTGTTCGGTGGAAG
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=205,22
PRIMER_LEFT_2_TM=59.588
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.7636
PRIMER_LEFT_3_PENALTY=0.283555
PRIMER_RIGHT_3_PENALTY=0.480050
PRIMER_LEFT_3_SEQUENCE=ACCCCAAATAAACCCTTTCATC
PRIMER_RIGHT_3_SEQUENCE=TGTTTGGAAGAGCTTCACTGTC
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=177,22
PRIMER_LEFT_3_TM=60.284
PRIMER_RIGHT_3_TM=59.520
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=0.7846
PRIMER_LEFT_4_PENALTY=0.283555
PRIMER_RIGHT_4_PENALTY=0.501074
PRIMER_LEFT_4_SEQUENCE=ACCCCAAATAAACCCTTTCATC
PRIMER_RIGHT_4_SEQUENCE=TTCACTGTCGTGAGTTCTCTCC
PRIMER_LEFT_4=45,22
PRIMER_RIGHT_4=164,22
PRIMER_LEFT_4_TM=60.284
PRIMER_RIGHT_4_TM=59.499
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_04241 Ps_contig_mira-and-tgicl-ass_franssen_32616
SEQUENCE=TCGGGGAGAGACGGAGGCAGAGGCACAAACAAAAAAACGTTCTAAACCCCAAATAAACCCTTTCATCACCGCCGCCGCCGTCTCTCTCTCTGCACCGTCGCCTTGCGTCAGAAAGATATTGCAGCTGTATCACGGTGCGAGATGGAGAGAACTCACGACAGTGAAGCTCTTCCAAACAGCAAGCCTTCCACCGAACAGGACATTATTGATGAGCTAAAATTGAGATCAAAGAAGTATCTCAGAGGAGAAGGTGGTAATTTGAAGGGTTTAAAGGATAAAAAGACTTAGGAGTCAGTTAGTTGCTAGAGAGAAATTATATGGAGAATCTGCGAAAGCTGCTGCCAAGGCTGAGAAATGGATTTTGCCTAGTGATCAAGGTTTTTTTGGAGGCTGAAGGCATAGAAAAGACATATAGGATTAAGCAGGAGACAATAGCCCGTGAAGTCGATATTTTAAGCTCAAGGAACCAATATGATATTACTTTACCAGAGCTTGGTCCATATACTCTAGACTTTACTTCAAGTGGCCGGTATATGGCTGTTGGCGGTCGTAAGGGCCACCTAGCAATTGTGGACATGATGAATCTGAGCCTGATCAGAGAGCTTCAGGTAAGAGAAACTGTGCGTGATGTGGTATTCTTACATAATGAGCTCTACTTTGCTGCTGCCCAGAAAAAGTATTCATACATATATAATAATGATGGCACAGAGCTTCACTGCCTAAAGGAACATGGTTCAGTGTTGAGGCTGCAGTTTCTGAAACACCATCTTCTCCTGGCTTCTACCAACAAATTTGGGATGCTTCTTTATCAGGATGTTACACTGGGTGATTTAGCTGGGAAATATCGAACTGGTTTAGGACGTACAAGTGTGATGGAAGTAAACCCTTACAATTCGGTTGTTTCTTTAGGTCACTCAGCCGGTACAGTTACTATGTGGAAGCCAACTAGTTCTTCTCCCCTGGTCAAGATGCTATGTCATCACGGGCCAGTCTCAGCATTGGCATTTCACCCAAATGGCCACACAATGGCCACAGCTGGTAAAGATAAAAAATTAAACTTTGGGACTTGAGGAAGTATGAGGCTTTTCAAACATTACCAGGCCATGACAAAATAAGTTTAGGAAAGTTTAGTCAAAAAGGCTTGCTTGCTTGTGGTA
TARGET=81,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4606
PRIMER_LEFT_PENALTY=0.283555
PRIMER_RIGHT_PENALTY=0.177008
PRIMER_LEFT_SEQUENCE=ACCCCAAATAAACCCTTTCATC
PRIMER_RIGHT_SEQUENCE=TCATCAATAATGTCCTGTTCGG
PRIMER_LEFT=45,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=60.284
PRIMER_RIGHT_TM=59.823
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.5890
PRIMER_LEFT_1_PENALTY=0.412035
PRIMER_RIGHT_1_PENALTY=0.177008
PRIMER_LEFT_1_SEQUENCE=CCAAATAAACCCTTTCATCACC
PRIMER_RIGHT_1_SEQUENCE=TCATCAATAATGTCCTGTTCGG
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=59.588
PRIMER_RIGHT_1_TM=59.823
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.5890
PRIMER_LEFT_2_PENALTY=0.412035
PRIMER_RIGHT_2_PENALTY=0.177008
PRIMER_LEFT_2_SEQUENCE=CCCAAATAAACCCTTTCATCAC
PRIMER_RIGHT_2_SEQUENCE=TCATCAATAATGTCCTGTTCGG
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=59.588
PRIMER_RIGHT_2_TM=59.823
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.6536
PRIMER_LEFT_3_PENALTY=0.412035
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=CCCAAATAAACCCTTTCATCAC
PRIMER_RIGHT_3_SEQUENCE=ATAATGTCCTGTTCGGTGGAAG
PRIMER_LEFT_3=47,22
PRIMER_RIGHT_3=205,22
PRIMER_LEFT_3_TM=59.588
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=0.6536
PRIMER_LEFT_4_PENALTY=0.412035
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=CCAAATAAACCCTTTCATCACC
PRIMER_RIGHT_4_SEQUENCE=ATAATGTCCTGTTCGGTGGAAG
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=205,22
PRIMER_LEFT_4_TM=59.588
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_04242 Ps_contig_mira-and-tgicl-ass_franssen_32616
SEQUENCE=TCGGGGAGAGACGGAGGCAGAGGCACAAACAAAAAAACGTTCTAAACCCCAAATAAACCCTTTCATCACCGCCGCCGCCGTCTCTCTCTCTGCACCGTCGCCTTGCGTCAGAAAGATATTGCAGCTGTATCACGGTGCGAGATGGAGAGAACTCACGACAGTGAAGCTCTTCCAAACAGCAAGCCTTCCACCGAACAGGACATTATTGATGAGCTAAAATTGAGATCAAAGAAGTATCTCAGAGGAGAAGGTGGTAATTTGAAGGGTTTAAAGGATAAAAAGACTTAGGAGTCAGTTAGTTGCTAGAGAGAAATTATATGGAGAATCTGCGAAAGCTGCTGCCAAGGCTGAGAAATGGATTTTGCCTAGTGATCAAGGTTTTTTTGGAGGCTGAAGGCATAGAAAAGACATATAGGATTAAGCAGGAGACAATAGCCCGTGAAGTCGATATTTTAAGCTCAAGGAACCAATATGATATTACTTTACCAGAGCTTGGTCCATATACTCTAGACTTTACTTCAAGTGGCCGGTATATGGCTGTTGGCGGTCGTAAGGGCCACCTAGCAATTGTGGACATGATGAATCTGAGCCTGATCAGAGAGCTTCAGGTAAGAGAAACTGTGCGTGATGTGGTATTCTTACATAATGAGCTCTACTTTGCTGCTGCCCAGAAAAAGTATTCATACATATATAATAATGATGGCACAGAGCTTCACTGCCTAAAGGAACATGGTTCAGTGTTGAGGCTGCAGTTTCTGAAACACCATCTTCTCCTGGCTTCTACCAACAAATTTGGGATGCTTCTTTATCAGGATGTTACACTGGGTGATTTAGCTGGGAAATATCGAACTGGTTTAGGACGTACAAGTGTGATGGAAGTAAACCCTTACAATTCGGTTGTTTCTTTAGGTCACTCAGCCGGTACAGTTACTATGTGGAAGCCAACTAGTTCTTCTCCCCTGGTCAAGATGCTATGTCATCACGGGCCAGTCTCAGCATTGGCATTTCACCCAAATGGCCACACAATGGCCACAGCTGGTAAAGATAAAAAATTAAACTTTGGGACTTGAGGAAGTATGAGGCTTTTCAAACATTACCAGGCCATGACAAAATAAGTTTAGGAAAGTTTAGTCAAAAAGGCTTGCTTGCTTGTGGTA
TARGET=1140,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04243 Ps_contig_mira-and-tgicl-ass_franssen_32617
SEQUENCE=GGTTGTTTCTTTTAGGTTCTACTCAGCCGGTACAGTTACTATGTGGAAGGCCAACTAGTTCTTTCTTCCCCCTTTCGTTCTAAGATGCTATGTCATCACGGGCCAGTCTCAGCACTGGCATTTCACCCAAATGGCCACACAATGGCCACAGCTGGTAAAGATAAAAAAATTAAACTTTGGGACTTGAGGAAGTATGAGGCTTTTCAAACATTACCAGGCCATGCAAATAGTTTGGAGTTTAGTCAAAAAGGCTTGCTTGCTTGTGGTAATGGTTCATATATCCAGGTTCTTAGGGATGCTTCTGGTGCACAGAACTATTCAAGGTACATGACTCATTCAATGGTAAAGGGTTATCAGATAGGGAAGTTAGCTTTCCGACCATACGAAGATGTTCTAGGCATAGGGCATTCAATGGGTTGGTCTAGTATTCTTATTCCAGGAGCAGGAGAACCCAACTTTGATTCATGGGTTGCGAATCCATTCGAAACACCTAAACAGAGAAGAGAAAAGGAAATAAAGTCTTGTATTGATAAGCTTCGATCCGAGACAATCATGCTAGATCCCAGCAAGATCGGTACTGTGAAATTTAACTAAAAAAAGAGAAGCCAACTGTGGAAGAGATGGATGCTGACATGGAAGCTGCTGTTGAAGCTGTCAAGGGCAAGAAACTGAAAAAGAAAACAAAAGGAAGAAGTAAGGCAGGTAAAATAATGCCAAAAAAGCAAGATGCAATTGCCACTGCAAAGAGGCCTTACTTAGAGCAGAAAATTCAAGAAGAGAAAAACATATCTAGTAAGAAACAGAAAACTAATGAGGGAGTTGAACTACCAAAGTCCTTACAGCGGTTTGCTCATAAGAAACCATCATCATAATTAAATTTGCCAAGTTAGTCGGCATTTTGAGGTTTTATTGTCGTGTAAGCTTTTTATTTGTATTTTGGGTTCTCTTTGATATTTGTAGCCCTTGGCCTTAGAAATCGATATTTATTGCCTTCCAGGTAAGGCATATCCAATTTTTGACCTTTAATTCNNCTATTCATTTTTATTTTCAATATTTATACACATATT
TARGET=251,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1488
PRIMER_LEFT_PENALTY=0.018437
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=TAAGATGCTATGTCATCACGGG
PRIMER_RIGHT_SEQUENCE=CACCAGAAGCATCCCTAAGAAC
PRIMER_LEFT=80,22
PRIMER_RIGHT=306,22
PRIMER_LEFT_TM=59.982
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.1000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.2604
PRIMER_LEFT_1_PENALTY=0.236882
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=ATTACCAGGCCATGCAAATAGT
PRIMER_RIGHT_1_SEQUENCE=TGAATCAAAGTTGGGTTCTCCT
PRIMER_LEFT_1=209,22
PRIMER_RIGHT_1=464,22
PRIMER_LEFT_1_TM=59.763
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.2604
PRIMER_LEFT_2_PENALTY=0.236882
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=ATTACCAGGCCATGCAAATAGT
PRIMER_RIGHT_2_SEQUENCE=GAATCAAAGTTGGGTTCTCCTG
PRIMER_LEFT_2=209,22
PRIMER_RIGHT_2=463,22
PRIMER_LEFT_2_TM=59.763
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=0.2729
PRIMER_LEFT_3_PENALTY=0.018437
PRIMER_RIGHT_3_PENALTY=0.254467
PRIMER_LEFT_3_SEQUENCE=TAAGATGCTATGTCATCACGGG
PRIMER_RIGHT_3_SEQUENCE=CAGAAGCATCCCTAAGAACCTG
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=303,22
PRIMER_LEFT_3_TM=59.982
PRIMER_RIGHT_3_TM=60.254
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=11.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.2810
PRIMER_LEFT_4_PENALTY=0.236882
PRIMER_RIGHT_4_PENALTY=0.044097
PRIMER_LEFT_4_SEQUENCE=ATTACCAGGCCATGCAAATAGT
PRIMER_RIGHT_4_SEQUENCE=ATTGAATGCCCTATGCCTAGAA
PRIMER_LEFT_4=209,22
PRIMER_RIGHT_4=412,22
PRIMER_LEFT_4_TM=59.763
PRIMER_RIGHT_4_TM=59.956
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=204
=
PRIMER_SEQUENCE_ID=SSR_04244 Ps_contig_mira-and-tgicl-ass_franssen_32621
SEQUENCE=ACTTTCCTTTTAAATACCAAAAAATTTAAACTTCCATTTCGAAATATATCCAAATTTGGACCCAACGCARNGNTAATCACTTCCAAACGCGTGTCTACAAAACCGAAACCCTGACTCTCACTTCCATTTCACCACCACCCTCACATCAACAACCAAACAAACAAAACCGTTGCGCAAATTCAAACTCATAGATCCGCCATGGCATCCCCCAACGAAAGCTTCGTCTACACCGCCAAGCTCGCCGAACAAGCCGAACGCTACGAAGAAATGGTTGAAGCGATGAAGAAAGTCGCTAAGCTTGACGTTGAATTGACGGTTGAAGAGCGTAATCTTCTGTCTGTTGCTTATAAGAATGTTGTGGGAGGACGTAGGGCCTCGTGGAGGATTCTTTCGTCGATTGAGCAAAGGGAGGAAACGAAAGGGAACGATGTGAATGTGAATCGGATTAAGGAGTATAGGAAAAAAGTTGAATCTGAGTTGTCTGATATCTGCACTGATATCATGGCTGTGATTGATGAGCATCTCATTCCTAATTCATCTGGTGAATCTAATGTCTTTTACTACAAAATGAAGGGTGACTATTATCGTTATCTGGCTGAGTTTAAGAGTGGGGATGACAGGAAAGAGGCTGCTGATCAGTCCTTGAAAGCATATCAGGAAGCTTCCACTGCTGCAGAGACCGAGTTACCTCCCACTCATCCTGTCAGGCTGGGTTTGGCTTTGAACTTCTCTGTCTTCTATTATGAAATCTTGAACTCTCCTGAAAGGGCCTGTCACCTTGCCAAGCAAGCATTTGATGTAGCCATTGCTGAGTTGGATTCTCTCAATGAGGAATCTTACAAAGACAGCACGCTGATTATGCAGCTTCTGAGGGACAACCTGAAATTGTGGACTTCTGACCTTCCTGAGGAAGGAGCTGAGGAACAAAAGGCCGAGTCTGCCCGAGCTCCTGTAGGAGATAATGCAGAGTAAAGTGACGGGTTTTAAAAGGGGTTTGTGGTGTGAATACAAAAGATTAGTCTCCATGCAAGTTCTGGTGGTTTTCTATTTCCATGTTTTCTAGTTCTAGGAAGTTTATAGTAATCCATTAGGATGTTTTAATTGAAGAGTTAGTGAGTCTCTAGGAAAATTATAAATCCTGAGCTCCTCTTCATTTGACAAGGGAATACATCATCATCAATGTTGCTTTAGGTTTCTTGTTTTAGCTATANNAAAGAAA
TARGET=154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04245 Ps_contig_mira-and-tgicl-ass_franssen_32633
SEQUENCE=ACTTTTCTAAAGTCTACAGTATATCACTCTGTTAAGTTTGTATAACCGCAAATTCGGGATGAATAATCTAATGATGAGTTTCTTATACATTTTTCACTCTAAGTTTCATGTAAGAATATTCCATCTTTAGTTTTATGAGCTCATCAAATTGACTATCAAAATAACTCTTTAAGCTGCATATCATTGTGTGAGACGAAAATTGTTGCACAACAGCTAGATCCCTCAAGACAAGTTATTTATTTACCTTAGGCATTTTTAGGCTCAATTATAACATCGAAACCGAATTCTTTTCCTGCTCTGAATGACTTTTTGAGTTCTTGGACCGTTTGAATAGTGCTAACCTTCTTGTCCTCGGCATCCAAGTTTTCCTTTTTTACATATTCAGCCATGGTACAATTGAGTATCTCTTGTATTGCAAACTTAGTGACGCGATCTTCCCCAAGCATCTCTAAAAGGAAGCTTTTTGGGATCTTTGATGATTTCCCTCCTTTTCGCATGCGAAATCCAGGAACTGGTGGTGCCTTACGACCTAAGTTAACGAGAATTCGGTCAAATACCTTCTGAGTTTGATCACCATTCAAGTTCACTCTTAGTTGTATCTTATCTTCATCCCGTGGTTCTTGTACGAGTACTGTGGCATCAGTTAAGAAGACTGAAATATCATTTGAATCTGTAATTGATGCTTCTAAACCTGAATTAACAGCTGAAATTGGCGTAGAGAGAATACGTAAGCTTGTTGTTGTTGTTCTCCATGGGGAGAGGGAGAGAGAAAAAGTGGCTGGTTTTGGTATTGAATTTGGAGTGGCGAGATTGGCGTAGAAGTTTGGAGAAGTGAATGAAGCAATGGAGTTGGAGGCACCAATCATCATCGTCTCTCTCTTTCTTTTACGCTATCGAGTCCCTTATCCGTTTTAATTTTGCGGTTTTGACATAGCATTAACAAAGTACGGCCACTCGATCAACCAATTCGACTTATTTCCGGTTTTTCAGTTAT
TARGET=734,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0920
PRIMER_LEFT_PENALTY=0.089381
PRIMER_RIGHT_PENALTY=0.002623
PRIMER_LEFT_SEQUENCE=TAACAGCTGAAATTGGCGTAGA
PRIMER_RIGHT_SEQUENCE=CTGAAAAACCGGAAATAAGTCG
PRIMER_LEFT=697,22
PRIMER_RIGHT=989,22
PRIMER_LEFT_TM=59.911
PRIMER_RIGHT_TM=59.997
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.0937
PRIMER_LEFT_1_PENALTY=0.089381
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=TAACAGCTGAAATTGGCGTAGA
PRIMER_RIGHT_1_SEQUENCE=AAGTCGAATTGGTTGATCGAGT
PRIMER_LEFT_1=697,22
PRIMER_RIGHT_1=973,22
PRIMER_LEFT_1_TM=59.911
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=277
PRIMER_PAIR_PENALTY_2=0.2804
PRIMER_LEFT_2_PENALTY=0.164639
PRIMER_RIGHT_2_PENALTY=0.115769
PRIMER_LEFT_2_SEQUENCE=TTATCTTCATCCCGTGGTTCTT
PRIMER_RIGHT_2_SEQUENCE=ATTCAATACCAAAACCAGCCAC
PRIMER_LEFT_2=600,22
PRIMER_RIGHT_2=795,22
PRIMER_LEFT_2_TM=59.835
PRIMER_RIGHT_2_TM=60.116
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.2937
PRIMER_LEFT_3_PENALTY=0.164639
PRIMER_RIGHT_3_PENALTY=0.129103
PRIMER_LEFT_3_SEQUENCE=TTATCTTCATCCCGTGGTTCTT
PRIMER_RIGHT_3_SEQUENCE=TTCTCCAAACTTCTACGCCAAT
PRIMER_LEFT_3=600,22
PRIMER_RIGHT_3=830,22
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=60.129
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=231
PRIMER_PAIR_PENALTY_4=0.3317
PRIMER_LEFT_4_PENALTY=0.089381
PRIMER_RIGHT_4_PENALTY=0.242357
PRIMER_LEFT_4_SEQUENCE=TAACAGCTGAAATTGGCGTAGA
PRIMER_RIGHT_4_SEQUENCE=GGGACTCGATAGCGTAAAAGAA
PRIMER_LEFT_4=697,22
PRIMER_RIGHT_4=901,22
PRIMER_LEFT_4_TM=59.911
PRIMER_RIGHT_4_TM=59.758
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_04246 Ps_contig_mira-and-tgicl-ass_franssen_32635
SEQUENCE=CTAGTCCCATCCNAACCATTCACATTTTCATTTTGAACAATTTCTTTCCTCTCTGATCTGCTTATAAATCTGCACTAAGCACACAAAAAAATGTCAATGGATGTGATGGCAAATGAACGTGTTTGTTATGTTCACTGCAACTTCTGCAACACTACTCTTGCGGTTAGTGTTCCATGCAGTAGCTTGCTGACTATAGTGACAGTTAGATGTGGGCATTGTGCCAATTTGCTTTCGGTTAACATGGGAGCATCACTTCAATCTTTTCCACCTCAACAAGACCCTCAGAAACAGCAGCTTATAAGCTGTCATCAAGAACCAATTAGCAGGAAGGAAGTTGTAGGATCATCATCATCATCTTCTTCAAAATGCAAAGCTTTTCAGCCTCATGAGCAACCTAGGACTCCTCCCATTCGTCCTCCTGAGAAGAGACAACGTGTACCTTCGGCTTATAATCGGTTCATTAAGGAAGAAATACAAAGAATTAAGGCTAGTAATCCAGATATCAGTCACAGGGAAGCTTTTAGCACAGCAGCCAAAAACTGGGCACATTTTCCTCACATTCACTTTGGGTTAAAGTTGGATGGTAGCAAGCAAGCAAAGTTGGACCAGGGGGTTGGGGACACTACTCAAAAGTCTAACGGATTCTATTGATACCTATAAAGTGGAATTAATAAGTCTATAATTAATTATCAATCAGTTGGTTTTTTCTTTGCTTTTAAGTGGAAAAAAAATGAGGTTGATCAAGTTTAATTTCCCATTCACACGACTGCATGCATGGGGGACTCTTCTCAAAATTAACCTTTGTTCTTCACCTTGTGTTTAAGTAATAGTACATGTATTTCCCTTTCTGATAATGATTTCTATTCTTGTGATTGCATTAGTTATATATTATGTTGAACTTAGGCACTTGTTGAGTAACAATATAAAGGTTCTTAACATGTGTTACGTTTTTTATT
TARGET=342,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3344
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.302618
PRIMER_LEFT_SEQUENCE=ATGGGAGCATCACTTCAATCTT
PRIMER_RIGHT_SEQUENCE=GTGCTAAAAGCTTCCCTGTGAC
PRIMER_LEFT=240,22
PRIMER_RIGHT=526,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.303
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.3429
PRIMER_LEFT_1_PENALTY=0.122257
PRIMER_RIGHT_1_PENALTY=0.220659
PRIMER_LEFT_1_SEQUENCE=CAAGAACCAATTAGCAGGAAGG
PRIMER_RIGHT_1_SEQUENCE=TGCTTGCTACCATCCAACTTTA
PRIMER_LEFT_1=309,22
PRIMER_RIGHT_1=592,22
PRIMER_LEFT_1_TM=60.122
PRIMER_RIGHT_1_TM=59.779
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=0.3590
PRIMER_LEFT_2_PENALTY=0.122257
PRIMER_RIGHT_2_PENALTY=0.236697
PRIMER_LEFT_2_SEQUENCE=CAAGAACCAATTAGCAGGAAGG
PRIMER_RIGHT_2_SEQUENCE=GCTACCATCCAACTTTAACCCA
PRIMER_LEFT_2=309,22
PRIMER_RIGHT_2=587,22
PRIMER_LEFT_2_TM=60.122
PRIMER_RIGHT_2_TM=60.237
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.3590
PRIMER_LEFT_3_PENALTY=0.122257
PRIMER_RIGHT_3_PENALTY=0.236697
PRIMER_LEFT_3_SEQUENCE=CAAGAACCAATTAGCAGGAAGG
PRIMER_RIGHT_3_SEQUENCE=TGCTACCATCCAACTTTAACCC
PRIMER_LEFT_3=309,22
PRIMER_RIGHT_3=588,22
PRIMER_LEFT_3_TM=60.122
PRIMER_RIGHT_3_TM=60.237
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.3590
PRIMER_LEFT_4_PENALTY=0.122257
PRIMER_RIGHT_4_PENALTY=0.236697
PRIMER_LEFT_4_SEQUENCE=CCAATTAGCAGGAAGGAAGTTG
PRIMER_RIGHT_4_SEQUENCE=GCTACCATCCAACTTTAACCCA
PRIMER_LEFT_4=315,22
PRIMER_RIGHT_4=587,22
PRIMER_LEFT_4_TM=60.122
PRIMER_RIGHT_4_TM=60.237
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_04247 Ps_contig_mira-and-tgicl-ass_franssen_32635
SEQUENCE=CTAGTCCCATCCNAACCATTCACATTTTCATTTTGAACAATTTCTTTCCTCTCTGATCTGCTTATAAATCTGCACTAAGCACACAAAAAAATGTCAATGGATGTGATGGCAAATGAACGTGTTTGTTATGTTCACTGCAACTTCTGCAACACTACTCTTGCGGTTAGTGTTCCATGCAGTAGCTTGCTGACTATAGTGACAGTTAGATGTGGGCATTGTGCCAATTTGCTTTCGGTTAACATGGGAGCATCACTTCAATCTTTTCCACCTCAACAAGACCCTCAGAAACAGCAGCTTATAAGCTGTCATCAAGAACCAATTAGCAGGAAGGAAGTTGTAGGATCATCATCATCATCTTCTTCAAAATGCAAAGCTTTTCAGCCTCATGAGCAACCTAGGACTCCTCCCATTCGTCCTCCTGAGAAGAGACAACGTGTACCTTCGGCTTATAATCGGTTCATTAAGGAAGAAATACAAAGAATTAAGGCTAGTAATCCAGATATCAGTCACAGGGAAGCTTTTAGCACAGCAGCCAAAAACTGGGCACATTTTCCTCACATTCACTTTGGGTTAAAGTTGGATGGTAGCAAGCAAGCAAAGTTGGACCAGGGGGTTGGGGACACTACTCAAAAGTCTAACGGATTCTATTGATACCTATAAAGTGGAATTAATAAGTCTATAATTAATTATCAATCAGTTGGTTTTTTCTTTGCTTTTAAGTGGAAAAAAAATGAGGTTGATCAAGTTTAATTTCCCATTCACACGACTGCATGCATGGGGGACTCTTCTCAAAATTAACCTTTGTTCTTCACCTTGTGTTTAAGTAATAGTACATGTATTTCCCTTTCTGATAATGATTTCTATTCTTGTGATTGCATTAGTTATATATTATGTTGAACTTAGGCACTTGTTGAGTAACAATATAAAGGTTCTTAACATGTGTTACGTTTTTTATT
TARGET=586,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1452
PRIMER_LEFT_PENALTY=0.045851
PRIMER_RIGHT_PENALTY=0.099308
PRIMER_LEFT_SEQUENCE=ACAACGTGTACCTTCGGCTTAT
PRIMER_RIGHT_SEQUENCE=CTTTTGAGTAGTGTCCCCAACC
PRIMER_LEFT=428,22
PRIMER_RIGHT=632,22
PRIMER_LEFT_TM=59.954
PRIMER_RIGHT_TM=59.901
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.3638
PRIMER_LEFT_1_PENALTY=0.302618
PRIMER_RIGHT_1_PENALTY=0.061184
PRIMER_LEFT_1_SEQUENCE=GTCACAGGGAAGCTTTTAGCAC
PRIMER_RIGHT_1_SEQUENCE=TAATTTTGAGAAGAGTCCCCCA
PRIMER_LEFT_1=505,22
PRIMER_RIGHT_1=796,22
PRIMER_LEFT_1_TM=60.303
PRIMER_RIGHT_1_TM=59.939
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.3672
PRIMER_LEFT_2_PENALTY=0.267873
PRIMER_RIGHT_2_PENALTY=0.099308
PRIMER_LEFT_2_SEQUENCE=CCTCATGAGCAACCTAGGACTC
PRIMER_RIGHT_2_SEQUENCE=CTTTTGAGTAGTGTCCCCAACC
PRIMER_LEFT_2=381,22
PRIMER_RIGHT_2=632,22
PRIMER_LEFT_2_TM=60.268
PRIMER_RIGHT_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=0.4019
PRIMER_LEFT_3_PENALTY=0.302618
PRIMER_RIGHT_3_PENALTY=0.099308
PRIMER_LEFT_3_SEQUENCE=GTCACAGGGAAGCTTTTAGCAC
PRIMER_RIGHT_3_SEQUENCE=CTTTTGAGTAGTGTCCCCAACC
PRIMER_LEFT_3=505,22
PRIMER_RIGHT_3=632,22
PRIMER_LEFT_3_TM=60.303
PRIMER_RIGHT_3_TM=59.901
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=128
PRIMER_PAIR_PENALTY_4=0.4417
PRIMER_LEFT_4_PENALTY=0.302618
PRIMER_RIGHT_4_PENALTY=0.139129
PRIMER_LEFT_4_SEQUENCE=GTCACAGGGAAGCTTTTAGCAC
PRIMER_RIGHT_4_SEQUENCE=CAGTCGTGTGAATGGGAAATTA
PRIMER_LEFT_4=505,22
PRIMER_RIGHT_4=768,22
PRIMER_LEFT_4_TM=60.303
PRIMER_RIGHT_4_TM=59.861
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_04248 Ps_contig_mira-and-tgicl-ass_franssen_32637
SEQUENCE=AATTTAGTAACCAAACGRAAGGTACTTTGACTCTCATCACTCCAATAACCGATATAAATTTTTTCTTAGCATTTATGCAATTACTGAATTTAGAAGGGGCTCCTTTTTATCTTCCCATTCGTTCAACCTCTGGAGTGATGCTTCCACCTCTTTGTTCCAGTCAGTTCTAAATGTGACCCACATCAGAATAATTGTCTGCATAGTAGTTCCACCAAGCATCCCTAGCCACAGTCCCTTAGCACCAAAATTGAAGTAAAAGCCTAGAATTACACCCAATGGTACACCAACTAGATAATAACAACCCACATTCACATAAGCAACAAAAGCTTGCCATCCACAACCAACAGCAACACCAGATAAGACTGGTTGAATGCCATTCAGAACAAGAGAAAAAGCTAGGATTGGACAAAGATCTGAGACCGCGGCTGCCACGACTTCTCCCTCCGTGAAAATATAGCTGATAACATCCCTTAATGCAATCACAACTACTGCCGAGATTACTGATACTAAAAATGATATCAATGTCACCACTTTCACCGAAAATGAAGCTGATTTTGGATTTCTTGCTCCTAGCTCGTTGCTCACCCTCACACTTGCAGCTGCGTTGAATCCAACTGAGACCATGAACACCCATCCAAGAACCGTGGTACAAATAGAAAGAGAATCAAGAGCCAATTCAGGCTCAGGAAGAAGCCCAGCAAGCAAAACCAGAATCTGAAAGTACCAAGATTCAAGACAGAGCATAACCGCGGAAGCCGCCGACAGTTTAAAAAACTCCGGCAAACCCGAAAACGCCTCCAAAGTAAAGCCATTCCACGTCTTCTTACACTTTTCACTTTTCACAATGTAAACAAACTGAGCAACAACAACAATCCACCACGACAAACTCAAAACCGAAGAAGCTCCCACTAATCCCCACCCAATCTTAAAAACAACAAGCCAACTTAGTCCGAGATGAACCACCAATGTTGCTGCTGCAATGTATGCACTTGGAAGAACTATACTCTGTGCTTGAAGAAACTTGGAAATCGGAAAGTTTATCGCATAAGCGAATATTTGTGGGATAAGCCCGTAAACGAAAAGCGCGGCAGAAGAAGAGATTCTTGGTGATTGTCCTATGGCTATCAGAATTGGTTTGCTAAAGATATAAATAACCGTTAAGAATAAACCGGTTATTGTTAGAAGAATTGTTGATCTTTGTAGGTAAGTTCCAAGCATTTCATATTTTTCTGCTCCATATGCTTGTCCACATAGAGTTTCAACTGCACTTCCCATACCCAACATGAGGCCATAAGCAAATATCTGAATACCGGTGTTCCCGAGCGACGCGGCGGCGAGCTCAAGGTTACCAAGGTGGCCGGAGAAGATTTGTGTGGCCATAGACATAACATAGTTGGTGAGATAAACAAACACGGCTGGAGCGGCTAAGAAGAAAAGGAGTTTAAGCTCAATCCACGAGGCGGAACGGATGCGGCGGAAGTATGGCACGGTGGTGTCCGATAGAATTCTCTCAAGCTTGCCGTCAGATTCATGGTGCGCCGGTGGCGTATGGCTGGGAGGTGATAAGAGTGGTCCTTCGGCGGANTTTTTTGTTCACTGAATCCATTTGAATTATTGCTGTACGGTCGATAACCTTTACTAAACGAATGGTTTATAT
TARGET=861,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04249 Ps_contig_mira-and-tgicl-ass_franssen_32639
SEQUENCE=GTCTTTGTTGTTTAAGTAAGAAAGATAAATGGTAAGNTCGAGAGTGTTAAAGCAAAGGGGCCTAGTGGTGGTGGTGGAAGAACTGAAAAACCAAAAGAAGAGTTTGGAAGTGGCGTGCAAGAATCTGAGAAAAACAAATTGGTTTTCTTTGAGGGATGCTCTTATAATTTCGATCTCGAGGATTTGTTGCGAGCTTCGGCCGAGGTTCTCGGAAAGGGTAGTTATGGAACTGCATATAAGGCCATCTTGGAGGAATCAACAACAGTTGTAGTGAAAAGGCTGAAGGAAGTTGTGGTCGGAAAGAGGGAATTTGAACAGCAGATGGAGATTGTTGGAAGCATTGGAAATCACCCGAATGTTGTTCCACTTCGCGCTTATTATTATTCGAAGGATGAGAAGCTTTTGGTTTGTGACTATTTCCCAAATGGCAATCTATCTATACTCTTGCATGGTACTAGGACAGGCGGAAGAGTTACCTTAGATTGGAACATGCGAGTAAAAATCACTCTCGGAATTGCTCGGGGAATCGCGCATTTACATTCAGGTTGGCGGTCCAAAATTCACACACGGGAATGTAAAATCCTCCAATGTCCTCGTAAACCAAGATAACGATGGCTGCATTTCTGATTTCGGCCTGACACCAATCATGAACGTTCCTGCGACCCCTTCTAGAACCGCAGGCTACCGTGCTCCCGAGGTAATCGAAACTCGAAAGCACACTCATAAATCAGACGTGTACAGTTTCGGTGTCCTCCTTTTAGAAATGCTTACAGGTAAAGCGCCACAACAGTCTCCAATACGCGATGACATGGTTGATCTGCCTAGGTGGGTTCAGTCTGTTGTTAGGGAGGAATGGACCGCCGAGGTTTTCGATGTGGAGCTAATGAGGTACCAAAATATTGAAGAAGAAATGGTGCAAATGTTGCAAATTGGCATGGCTTGCGTTGCCAAGGTTCCGGATCTGAGGCCTAACATGGAGGAAGTAGTGAGGATGATTGAAGAAATTCGACAATCTGACTCCGACAACCGACCGTCTTCTGACGAGAATAAATCCAAGGATTTAAATGTYCAAACACCATGATTAACTATGCTGTTNTNTTTTTCTGNGTTACAAA
TARGET=65,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04250 Ps_contig_mira-and-tgicl-ass_franssen_32640
SEQUENCE=TAAGACAGTAGGTAATCAACGCAGAGTACTCGGGGAGAAAAGAGGATAATGGCTTCGTTTTCGGTTTCTCCTCTGTTATCCATCACACTCAGAGCACCATCTCTCACCGCCTCAAAACCGTCTTCACTCTCTCTCAATTCCTCCAACCTTCGTTCTCCATTGCTTCATTGCTCATTCGCTTCCACGCATTCCTCTCTCTCTTTCCACTCTTCCTCCTTTTCAGGTTTGTCATTGGGATTAAGTTTGACATCGAATGTTGGATCTAGAAGACAAAGCGGTCGCGGCTTGGTTATTGTAGCAGCAGGAAGGACTGCTCTTAATCTAACCAAGAGAAGTAGATCACGAAAATCATTGGCACGCGTTCATGGTTTCCGCATACGAATGAGGACAACAAGTGGAAGAGCTCTCTTAAAGCGACGACGTGCCAAGGGACGAAAAATCCTTTGTACTAAGACCCATTCAAATAGTGGCAAATAGTCACAAAAAATGGCCTAAGAATTATTCTATTTACATGTATTGACTTCACAAACTTGTCTGTATTTCAATTTTTTGTAACACATGCTACATTTTGATCTAAGTTCGACTAAATATCAACGGCATATTGTGACTAATCCTTCGAAACTCTTTTTTAGTTGAAACAATTACTAAAGAGGAGAGATGAACATATTTAGCATGGGAAGGGATTTCATGATTCTTCTTAAAGAGCCATCTTCTTCTCTCTCCCTTTAGTGTTATTCGATATACATTTGT
TARGET=192,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0560
PRIMER_LEFT_PENALTY=0.032476
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=GTACTCGGGGAGAAAAGAGGAT
PRIMER_RIGHT_SEQUENCE=ATGACAAACCTGAAAAGGAGGA
PRIMER_LEFT=25,22
PRIMER_RIGHT=231,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.0804
PRIMER_LEFT_1_PENALTY=0.056887
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=CACTCAGAGCACCATCTCTCAC
PRIMER_RIGHT_1_SEQUENCE=ATGACAAACCTGAAAAGGAGGA
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=231,22
PRIMER_LEFT_1_TM=60.057
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.0804
PRIMER_LEFT_2_PENALTY=0.056887
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=CACACTCAGAGCACCATCTCTC
PRIMER_RIGHT_2_SEQUENCE=ATGACAAACCTGAAAAGGAGGA
PRIMER_LEFT_2=83,22
PRIMER_RIGHT_2=231,22
PRIMER_LEFT_2_TM=60.057
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.0804
PRIMER_LEFT_3_PENALTY=0.056887
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=ACACTCAGAGCACCATCTCTCA
PRIMER_RIGHT_3_SEQUENCE=ATGACAAACCTGAAAAGGAGGA
PRIMER_LEFT_3=84,22
PRIMER_RIGHT_3=231,22
PRIMER_LEFT_3_TM=60.057
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=0.0804
PRIMER_LEFT_4_PENALTY=0.056887
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=TCACACTCAGAGCACCATCTCT
PRIMER_RIGHT_4_SEQUENCE=ATGACAAACCTGAAAAGGAGGA
PRIMER_LEFT_4=82,22
PRIMER_RIGHT_4=231,22
PRIMER_LEFT_4_TM=60.057
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_04251 Ps_contig_mira-and-tgicl-ass_franssen_32641
SEQUENCE=CTGTGTAGTATAGGCCTAAACTTTCTTCATATCCACCAATTATTTCAAACACATCCAAGAAGGACCATACAAAATAACCTTTTATATTTGATCCACTCCTTATTGCTTTAGCTATGCTCCCGATGTATTTATGCAAGGATTTTATCCTAGAAAAATCATTCAATGATGAACTGTGAGGTGTTTGATGGCCATTTTCATGTATATAAATTGGAAATTTTCCATAATCATTCTTCAAGGAATCTAGCACCGTGTTTATAGATGTATCATTTTCGGGAAGCATTGTAATTTCTGCGTCCATATCTGGTATTAAATCTATATCTTTCATGTTCAGGCTTTTGGAATGGTTGTTAACAGAAAATGTGCTGTAAAAGTTAATTCCTAAGAAGTCAATTGAGTTTCTGATCAGATTTGATTCCTCTTTAGTGAAGGAAGGAAGCCTTGAGCCAATATTCTTTTTCATTATATCAGGATAATCACCAAATGTTAATGGATTTAGTATCCACCCCATCCAAAAGTCTTGGGCCCCTTGAGTGGCAATAATATCTCCACTAGTATTTGTCACTGGAACAAAACTAATAGTAAGAAGATTCAACCCAATAAACCCGTGTTGTATTCCCTTATATTTCTTGTTATACAATCTTGCAACCGAAGCATGTGCTAACAGCATATGATGTGCTGCCAAATAAGGCTCGGTTGAAGAATTTCCTCTTGAGCAGTTTCCTAGAGAGAAAGGAGAACACCTCTGAGGTGGTGCTGCTCCAATACCATAGCCAACCATTGCAAACACATTAACCTCATTCAGTGTGGTCCAATACTTAACTCTATCACCAAATTCTCTGAAGCATACATCAGCATATGCTGTGAAATCATACACAACATTTCTACTAATCCATCCTTCATATTCGTCCTCAAGTGTTTGTGGTAGATCCCAATGGTGCAATGTAACATGTGGTTGGATCCCATGGCTTGTCAGTTCATTGATGAGGTTGTTGTAATATTCAAGTCCTTTGGGATTGATTGCTCCTTTTCCATCTGGAATAAGTCTTGACCATGATATTGAAAATCTATAGGCATCTAAACCCATATTAGCCATGAGTTGCACATCTTCCTTATATTTGTGATATCCATCGCATGAAACATCTCCATCACCTTTGTAAATATTCGCATTTCCAGCGTGAGAAAAGGTATCCCATATGCTTGGTTTCCTACCATCTTCATTTGCTGCACCTTCAACCTGGTAAGCAGAGGTTGATGCACCAAAGACAAGTCGGAAGGGACTCATCTCTGCTACAA
TARGET=425,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3303
PRIMER_LEFT_PENALTY=0.261998
PRIMER_RIGHT_PENALTY=0.068302
PRIMER_LEFT_SEQUENCE=TCTTCAAGGAATCTAGCACCGT
PRIMER_RIGHT_SEQUENCE=CTTTTGGATGGGGTGGATACTA
PRIMER_LEFT=228,22
PRIMER_RIGHT=514,22
PRIMER_LEFT_TM=60.262
PRIMER_RIGHT_TM=60.068
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.3000
PRIMER_RIGHT_END_STABILITY=4.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.3526
PRIMER_LEFT_1_PENALTY=0.261998
PRIMER_RIGHT_1_PENALTY=0.090580
PRIMER_LEFT_1_SEQUENCE=TCTTCAAGGAATCTAGCACCGT
PRIMER_RIGHT_1_SEQUENCE=TGGATGGGGTGGATACTAAATC
PRIMER_LEFT_1=228,22
PRIMER_RIGHT_1=510,22
PRIMER_LEFT_1_TM=60.262
PRIMER_RIGHT_1_TM=59.909
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.3000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.5658
PRIMER_LEFT_2_PENALTY=0.497481
PRIMER_RIGHT_2_PENALTY=0.068302
PRIMER_LEFT_2_SEQUENCE=AAGCATTGTAATTTCTGCGTCC
PRIMER_RIGHT_2_SEQUENCE=CTTTTGGATGGGGTGGATACTA
PRIMER_LEFT_2=274,22
PRIMER_RIGHT_2=514,22
PRIMER_LEFT_2_TM=60.497
PRIMER_RIGHT_2_TM=60.068
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=4.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=0.5658
PRIMER_LEFT_3_PENALTY=0.497481
PRIMER_RIGHT_3_PENALTY=0.068302
PRIMER_LEFT_3_SEQUENCE=GGAAGCATTGTAATTTCTGCGT
PRIMER_RIGHT_3_SEQUENCE=CTTTTGGATGGGGTGGATACTA
PRIMER_LEFT_3=272,22
PRIMER_RIGHT_3=514,22
PRIMER_LEFT_3_TM=60.497
PRIMER_RIGHT_3_TM=60.068
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=4.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.5881
PRIMER_LEFT_4_PENALTY=0.497481
PRIMER_RIGHT_4_PENALTY=0.090580
PRIMER_LEFT_4_SEQUENCE=GGAAGCATTGTAATTTCTGCGT
PRIMER_RIGHT_4_SEQUENCE=TGGATGGGGTGGATACTAAATC
PRIMER_LEFT_4=272,22
PRIMER_RIGHT_4=510,22
PRIMER_LEFT_4_TM=60.497
PRIMER_RIGHT_4_TM=59.909
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_04252 Ps_contig_mira-and-tgicl-ass_franssen_32647
SEQUENCE=TTCGGGGGTCGTCGACGGTACTCGAACATTCCAAAACACTTCTGGTTTCGACCAACTTGAACCCTCCTGCCATAGATCAGAAAATCAATTTTCTGATCATTCTCTTCTTCTCGCCCTTTCTATTTTTTTCTTATCTTCTCAGGTTTTTCATATTTCCAGAGTAAGCATCACCACTCACACAAGAATGGATCATCAAGGTCATGGCCAAAACCCAGCTATGGGAGTTGTTGGTAGTGGACAAATGCCCACATATGGTTCTAATCCATACCAGCCCAACCATATAACTGGGTCACCTGGGATGGTTGTTCCCTCTGTTGGGAATATGCAGCCCGGTGTTCCACCCGACGGAACTCAGCTGGGACAACATCAACTTGCTTATCAGCATATTCATCAGCAACAACAGCAGCAGCAGCAGCAACAACTCCAGAGCTTTTGGGGAACTCAATATCAAGAAATCGAGAAGGTAACTGATTTCAAAAACCACAGCCTTCCCTTGGCAAGGATCAAGAAGATTATGAAGGCGGATGAGGATGTGAAGATGATATCAGCTGAGGCGCCGGTTGTATTTGCAAGGGCGTGCGAAATGTTCATATTAGAGTTAACCTTGCGTTCTTGGACTCACACTGAAGAGAACAAAAGGCGAACACTTCAAAAAAATGATATTGCAGCAGCCATCACCGGAACTGATATCTTTGATTTCTTGGTTGATATTGTGCCCCGGGAGGACTTGAAAGATGAAGTGCTTGCATCAATACCAAGAGGGCCAATGCCTGTTGGAGGGCCTGCTGATGGAATTCCTTATGGCTATATGCCACCTCATCACGCACAGCAAGCTGGAAATCCCGGTCTCATGATGCATAATCCAAACATGTATGCTCCACAGTCTCATCCGTACATGGCACCTCAAATGTGGCCTCAACCACCAGAGCAACCACCATCACCCTCAGATCATTAGCTACGCATTATGGAAGTGTCATGCTTCTTAAGGTGAACTTTGGTGCAATAAAATAGTTCTGTAAGGGTTGAAAGTTGTAAGTTTTTTTTAGTACTGACCATTTCAATACATCACAGTAAAATTTAATTCTGCATCTTTAAAATTTCAGTCATTTTTCTTAATGGAATTGAATGTTACTGTATTGGAAATATACAGATGAATAACCAGTTATGGTTAATGATTCATCAGTTATTAGCACAAAATGTATCTGAATACAGGTNTCGGTCTTATACTCTTATTTTGTATTTTRCAGTAG
TARGET=401,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04253 Ps_contig_mira-and-tgicl-ass_franssen_32651
SEQUENCE=GCTCATATCCGGTTTTCTTAGAATTATAACTCAAAGTTTCATTTTGTCTTCACTTAGGAAGATTTCACGTACGCAAGCATGTGACACTGTTGCAACTTACGGTTCTCTTCAATAATAAAAAAAATAGTAACTAATCCACAATGTATCATCCAATTACAGAGGAAGATCGAATGGCTCCAGCCGCTGTCCTCTCAATCTACTCGACCGGTACATTCCGCTGAGCCTGAACATCAGCAGTTTGTTTCTAGTACACCTGATAACTTCACTTATGCCATTGGCATGTCCAGTTCTCCTTGTATTGATGCTACAAATGGTGGAGTAATGAGGGATGTTAAGCATCAAAGTTGTGGGATTCTCCTTCTCGTGATGATGGTCCAGAAGCATTATTGAAGTGCAGCTAAAACTTTACAGGGACACCATCAATATCAAGGTCTAAAATGAAGATGGATGATACTGCTGCCGTTGAGATTGTGCAACAATCCTCTGGTGTTTTGAACATGATAGGAACGGCCACACCACCACCTACAATTCCATTTTAACTTGCAGAATATTGCTCTGCACCATTTGACAGAATGTTAATACTCCCTGTAGAATTTCTCCAAATTTTACGTTCGAGTGGGCTTCGAATGGGTTTGTTTTCCATCCGTTACACAATCATGGTGCACCGTCCTGCAGTACACCAACAGCTGCACCAAAGAACCATCTCAGTTCAGGAAATTGCGGCAACAACGAACATAACCACGTGGACCCAATCATTCCGCAACGACAGCAGCCAACCTGTAGAAATGGCCAAACCCTGCAGTAACCATAGACAATAGCAGCGAGTGAGCTTTCCTACGGTTTCCACGCTTAACGGAATAACCTGCAAGAAAATAAATAAATAACAAG
TARGET=871,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04254 Ps_contig_mira-and-tgicl-ass_franssen_32652
SEQUENCE=CGNRCGTCGGGGATTCAGTCTTTATTCATTCTTCAACCAATCCATTCCCGCATCGCACCTAACGTTGTTCTCCGGCCACCGTCTACGACGGAGCGCCGAAATCTCTCTCGTTGAATCTGTTAACCCGGGGCTTGTGAAGCTTTAGGCAAACATCCATGGATAGAGTCTTGAACAAGCTCCGCAATTTGGATGCGTACCCTAAAGTCAATGAAGATTTTTATAACCGCACGCTCGCCGGCGGGGTCGTCACCGTTGTATCTGTCACCGTTATGCTTTTCCTTTTCTTGTCCGAGCTAAGATTATATCTCTACACTGTTACAGAGAGTAAGCTTTTGGTGGATACTTCAAGGGGAGAAACCTTGAACATCAATTTTGATGTCACTTTTCCTTCTGTTCGATGTTCAATACTCAGTTTAGACACAATGGATATCAGTGGCGAGCGGCATCATGATATACTACACAATATAATGAAACAAAGAATTGATGCTAATGGTAATGTGATAGAAGCTAGGAAGGAAGGAATTGGTGCACCCAAGATTGAAAGACCCTTACAAAAACATGGTGGCAGACTTGAACATGATGAAAAATATTGTGGTTCATGCTTTGGTGCAGAAGAGTCAGATGACCACTGTTGTAATAATTGTGAAGAGGTTCGGGAGGCATATAGGAAGAAGGGCTGGGCAATGTCAAATATGGATTTGATCGACCAGTGTCAAAGAGAGGGTTTTGTGCAGAAGGTAAAGGATGAAGAAGGTGAAGGATGTAATATTCATGGATCTCTTGAAGTGAATAAAGTGGCAGGAAATTTTCATTTTGCAACCGGGCAAAGCTTTCTTCAGTCAGCTATCTTTCTGGCTGATCTGCTTGCTTTGCAGGATAATCATTATAATATAAGCCATAGGATCAACAATTTAAGTTTTGGTCACCATTACCCCGGATTGGTTAATCCTCTTGATGGGGTAAAATGGTACCAAGGAACTAGTCATGGCATGTACCAGTATTTCATAAAGGTCGTCCCTACAGTATACACAGACATCAGAGGCCGAGTTATACATTCGAATCAGTATTCTGTGACTGAGCATTTCAAGAGTTCAGAACTAGGTGCTGCAGTTCCTGGGGTTTTCTTCTTCTATGACATATCGCCAATAAAGGTCACCTTTAAAGAGGAACATATTCCATTTTTACATTTCTTGACCAATATTTGTGCAATTATTGGAGGTATATTCACAATTGCTGGAATAGTAGATTCGTCAATATATTATGGTCAGAGAACAATAAAAAAGAAGATGGAGATTGGAAAATATAGATAGTGGAGTCAGGTCACAACATCCTTCCTATATGGATATGCTCTAATGTCCCGGTTGAATCAGCTCTTGACTTGTGCTTGGTTGGTTGGTCAGTGCATGCGCGTTGCAATATAGCACCGCGATTAAGCAGAGCCGTTGATTCAGTTGATTAATGAATTGCATCTTTGGAACTTGATGATAGCAGCTCTAGCCATTTTCATGTATTTTGAATCATTTGTGGTCGTTAAGTTATAATCCAAATATTTTGGTCCCTTTCCTATATTAGATTTTCATAAGGTGTAGAAGTCTGTGTTTTGGAATGAGATTTGAAGAAAACTTGCAGGATTATGTTAACTTTTTTATATGATTGTTTTAAAATTAAAAATGTTCCAAGTT
TARGET=510,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04255 Ps_contig_mira-and-tgicl-ass_franssen_32652
SEQUENCE=CGNRCGTCGGGGATTCAGTCTTTATTCATTCTTCAACCAATCCATTCCCGCATCGCACCTAACGTTGTTCTCCGGCCACCGTCTACGACGGAGCGCCGAAATCTCTCTCGTTGAATCTGTTAACCCGGGGCTTGTGAAGCTTTAGGCAAACATCCATGGATAGAGTCTTGAACAAGCTCCGCAATTTGGATGCGTACCCTAAAGTCAATGAAGATTTTTATAACCGCACGCTCGCCGGCGGGGTCGTCACCGTTGTATCTGTCACCGTTATGCTTTTCCTTTTCTTGTCCGAGCTAAGATTATATCTCTACACTGTTACAGAGAGTAAGCTTTTGGTGGATACTTCAAGGGGAGAAACCTTGAACATCAATTTTGATGTCACTTTTCCTTCTGTTCGATGTTCAATACTCAGTTTAGACACAATGGATATCAGTGGCGAGCGGCATCATGATATACTACACAATATAATGAAACAAAGAATTGATGCTAATGGTAATGTGATAGAAGCTAGGAAGGAAGGAATTGGTGCACCCAAGATTGAAAGACCCTTACAAAAACATGGTGGCAGACTTGAACATGATGAAAAATATTGTGGTTCATGCTTTGGTGCAGAAGAGTCAGATGACCACTGTTGTAATAATTGTGAAGAGGTTCGGGAGGCATATAGGAAGAAGGGCTGGGCAATGTCAAATATGGATTTGATCGACCAGTGTCAAAGAGAGGGTTTTGTGCAGAAGGTAAAGGATGAAGAAGGTGAAGGATGTAATATTCATGGATCTCTTGAAGTGAATAAAGTGGCAGGAAATTTTCATTTTGCAACCGGGCAAAGCTTTCTTCAGTCAGCTATCTTTCTGGCTGATCTGCTTGCTTTGCAGGATAATCATTATAATATAAGCCATAGGATCAACAATTTAAGTTTTGGTCACCATTACCCCGGATTGGTTAATCCTCTTGATGGGGTAAAATGGTACCAAGGAACTAGTCATGGCATGTACCAGTATTTCATAAAGGTCGTCCCTACAGTATACACAGACATCAGAGGCCGAGTTATACATTCGAATCAGTATTCTGTGACTGAGCATTTCAAGAGTTCAGAACTAGGTGCTGCAGTTCCTGGGGTTTTCTTCTTCTATGACATATCGCCAATAAAGGTCACCTTTAAAGAGGAACATATTCCATTTTTACATTTCTTGACCAATATTTGTGCAATTATTGGAGGTATATTCACAATTGCTGGAATAGTAGATTCGTCAATATATTATGGTCAGAGAACAATAAAAAAGAAGATGGAGATTGGAAAATATAGATAGTGGAGTCAGGTCACAACATCCTTCCTATATGGATATGCTCTAATGTCCCGGTTGAATCAGCTCTTGACTTGTGCTTGGTTGGTTGGTCAGTGCATGCGCGTTGCAATATAGCACCGCGATTAAGCAGAGCCGTTGATTCAGTTGATTAATGAATTGCATCTTTGGAACTTGATGATAGCAGCTCTAGCCATTTTCATGTATTTTGAATCATTTGTGGTCGTTAAGTTATAATCCAAATATTTTGGTCCCTTTCCTATATTAGATTTTCATAAGGTGTAGAAGTCTGTGTTTTGGAATGAGATTTGAAGAAAACTTGCAGGATTATGTTAACTTTTTTATATGATTGTTTTAAAATTAAAAATGTTCCAAGTT
TARGET=1385,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04256 Ps_contig_mira-and-tgicl-ass_franssen_32653
SEQUENCE=CAACTATCACTAATCTGGCCACTTCAATAATATTAAAAATTAGTATTTCTTATTTACCCTACGATGAGTTTTCTTGGTTTCTATTCTCTTTTCTAGATGCAGCAAAATTTTCTAGAGAATGCATGAACTTAGAGAGAGAGAGATGGTATAAATAGTTACTGGAACAGGTTTTATGGTATGCGGTTCTCAATCCTATCCTCTCAAGCTGTTTCGGGCGCATGGCTCTTAGATTGTACTGCTTTTTCAGCATCCGATGGACCGACAATATCATTGACTGTTTCAATCTCCAAGCTGGTGGTAGTCGCGCTTTTTCTATTCCTTCGATCCTCTCGAAATGGATATTCGTCGGCTTCAAGCATCCTAACAACATGACTCATTTTGGGCCTTTTTTCTGCATCGGGATCAATACATCTAAGAGCGACCAGAAGCGAACGCTTTAAAGCACGCGCAGATGGTTTAACTTCGAGTCTCAAATCCACAACTTCCTCGGCTCTCCTTGCCCCCACCATTATCTTGAGCCACTCAACAAGATTAACCTCATCAGAAGGACGCGAATAATTTACAGGGTCCCTTCCGGTAACGGCTTCAAGCAGGAGGACACCAAAGCTGTAAATGTCACTCTTCTCGTTTAACAAACCGCTGTTGGCATACTCTGGTGCTACATAGCCAAACGTTCCCATTACTCGAGTAGTTATGTAACTTTCTCCTGATTCCAAAAGTTTGGCCAAACCGAAATCAGAAACTTTCGCGTTGAACTCCTTGTCGATCAATATATTGCTGGACTTTATATCTCGGTGAATAACTTTCGGTTCTATTGCTTCATGTAAGTAAGCAAGCGCTTTGGCTGTGCCGAGAATAACTTTCATGCGGGCCTCCCAGGTAAGTGTTCCAAGTTTGTCTCCGTGTAACCACTGTTCTAAGTTACCGTTGTTCACGTATTCATACACCAGCAGCCTGTGAACTCCTTCTACGCAATATCCAAGTAAGCGCACGAGATGCTTATGTCTAACATGGCCTATAGCTTCCACTTCAACCCGGAATTCTCTCTCTGCTTGTCCCAGGTTGTTAAGAAGTTTCTTCACGGCAACATCGGTCCCATTGATCAGCCTGCCCCTGTAAACAACTCCATATCCACCCTCGCCGACAATGTTATCCTTCGAAAAATAATGAGTTGCTTGTTCAAGATCTCTAAGTGTAAACCAGTGACCCCACCCAAGATGCGAAAATTCTGGCAAGCCAATCAAAGGAGAGGTGGTTGTTGGCCCTCCTCCATACGACAATGTAGATCGCTTCTTGAAATTCCCGGAGCTCCCTTCTTCCCATGACAGTGAACTTAATCCTCTCTCGTGATGATAAACCGAGCTGCACTGACTAACACTATCAGGATCACTGAATTTGCCCGTCTTCATACGAACCGACATCTTGTCCAAATTACTATCATTAGGAACAAACACATCTCCATATTGAACATGAGAATTCTGCACCCCGACAATGTCAACATTGATATCTTTCGACACATTCGGTATTTGGGGCTTGTCGAGAGATCTCCTACTCCTAGACTTCCTCCGAAACATCACCCATACAGACAATATACAAAGAATCAGCACTATAAAGACTCCAACACCTATCCCAATCAAAACCCATCTTTTCAAACCCAAAACTGATAAGTCTTCTYCGATAACTCAACATTCAACGAATCATTTTGATGACATA
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04257 Ps_contig_mira-and-tgicl-ass_franssen_32659
SEQUENCE=CACCATGAGAGACCTGCAGAAATAAACCGGGAACAATCCCTCTGTAGAGTGCACTAAATCCTTCTTCCCTCATTATGGTCCTAAGTGCATCTCTAGAGCCAAAGTACAAAAGACATGAACAAAACAACAACAACCAAGTCYTATCCATATAGCCCAGAGTAAGGTCGTGTCTGATGAAGAGGAGTCTGAAGCTGCAATCTTGTCTTCACTAGCCAAACAGGATTTGTGCAGAAGCAAACCAAAGCTCCTGCTTCCGCAGCTGAGGCAAGATGAAGACCTGGGCTCAACTCCTCCTCTCTGCTCCTAGCATATCTTTGTTTAGCTTTGTCATAGAAAAAGAAATATAAACCCCAGGATATAGTTGACCCAAGAACCCCAGGAAGAAAGCCTGCATATAGCCCTCTTAATCCCTCGGAGCGAGTAATGGTGAAATTGCGTGAGCGGTGTTCTTGTAACCGGGAAGAT
TARGET=123,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04258 Ps_contig_mira-and-tgicl-ass_franssen_32663
SEQUENCE=CGGTTTTAAGTTTTCGTTCATCCGCTTCTGCAGCTTGAATGATGACTTTGCACCTGATGGCAGGATAGGTTAGACAGGTAGCAATGCTCTTTGAAATTGCACCTAAAAGAAAGGCCATAAACGCAGACAGTGATGCTGGAGATACTTCTTTCTCAGCGGTGTTTTGTTTATTCGTCAGTACTCGCTGTTTCAGCTGATCAAACACTGTATACTGAATTGCAGGGTTTGAGGTCAGAAGCAAGGAAACGCTAAGGCCGTCGAATATATCACTCCATGAGCCTTCAGTAAGAGTTTTCAGTAGTCCTTTAGACTTTCCAAAGGCACTTGTCTGCATCCTTGAAGAGGCTGTATCCAGGGGCTGAGTTGTAATGGCTGTGACAGCCCCAGCAGCAGCAGCGATTAACAAGTTTGCTCTTGTTCCAATGGATTTATATCCACTTTTTCCAGGTACAGCCTTTTAAAGTAACTGTAGCCATAGAAGTAGATAAACTGTGAAATGAAGGTATTGAAGATTCTTCGTTCCAAGGCCTTGGTAAAGGGAAAGCACCTGACGGTTAGATACTGCCTCCCATAACACATCAGAGAGGTTCTCGTACTTTACGATTACCATGAGAACGTGCCTCTGCTTGATACTTAGTCTTGCAGGTATACAGGTGGAGATATTGTA
TARGET=385,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1211
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.025566
PRIMER_LEFT_SEQUENCE=CATCCTTGAAGAGGCTGTATCC
PRIMER_RIGHT_SEQUENCE=AGAGGCACGTTCTCATGGTAAT
PRIMER_LEFT=331,22
PRIMER_RIGHT=623,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.1691
PRIMER_LEFT_1_PENALTY=0.143555
PRIMER_RIGHT_1_PENALTY=0.025566
PRIMER_LEFT_1_SEQUENCE=TATCCAGGGGCTGAGTTGTAAT
PRIMER_RIGHT_1_SEQUENCE=AGAGGCACGTTCTCATGGTAAT
PRIMER_LEFT_1=348,22
PRIMER_RIGHT_1=623,22
PRIMER_LEFT_1_TM=59.856
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.2165
PRIMER_LEFT_2_PENALTY=0.190908
PRIMER_RIGHT_2_PENALTY=0.025566
PRIMER_LEFT_2_SEQUENCE=GCTGAGTTGTAATGGCTGTGAC
PRIMER_RIGHT_2_SEQUENCE=AGAGGCACGTTCTCATGGTAAT
PRIMER_LEFT_2=357,22
PRIMER_RIGHT_2=623,22
PRIMER_LEFT_2_TM=59.809
PRIMER_RIGHT_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=0.2467
PRIMER_LEFT_3_PENALTY=0.095574
PRIMER_RIGHT_3_PENALTY=0.151106
PRIMER_LEFT_3_SEQUENCE=CATCCTTGAAGAGGCTGTATCC
PRIMER_RIGHT_3_SEQUENCE=GAGGCAGTATCTAACCGTCAGG
PRIMER_LEFT_3=331,22
PRIMER_RIGHT_3=567,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=0.2616
PRIMER_LEFT_4_PENALTY=0.235991
PRIMER_RIGHT_4_PENALTY=0.025566
PRIMER_LEFT_4_SEQUENCE=GCATCCTTGAAGAGGCTGTATC
PRIMER_RIGHT_4_SEQUENCE=AGAGGCACGTTCTCATGGTAAT
PRIMER_LEFT_4=330,22
PRIMER_RIGHT_4=623,22
PRIMER_LEFT_4_TM=60.236
PRIMER_RIGHT_4_TM=60.026
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_04259 Ps_contig_mira-and-tgicl-ass_franssen_32664
SEQUENCE=AAGATACTACACCTACCCACCACCTTGGCACCTGTGTGTTTGATTCGGTGGGAAGACGAAGAAGATGAACGTGGATCTGGAATCCTTGGCCGAAGCCACTTCTGGAGCTATAGGATCATTGCTCAGTTCAACAATTCTCTACCCACTTGATACCTGCAAGACTAAGTATCAAGCAGAGGCACGTTCTCATGGTAATCGAAAGTACAAGAACCTCTCTGACGTGTTATGGGAGGCAGTATCTAACCGTCAGGTGCTTTCCCTTTACCAAGGCCTTGGAACGAAGAATCTTCAATCCTTCATTTCACAGTTTATCTACTTCTATGGCTACAGTTACTTTAAAAGGACTGTACCTAGGAAAAAAGTGGATATAAAATCCATTGGAACAAGAGCAAACTTGTTAAATCGCTGCTGCTGCTGGGGCTGTCACAGCCAATTACAACTCCACGCCCCTGGATACAGCCTCTTCAAGGATGCAAGAACAAGTGCCTTTGGGAAGTCTAAAGGACTACTGAAAAC
TARGET=405,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0409
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=CACGTTCTCATGGTAATCGAAA
PRIMER_RIGHT_SEQUENCE=GTGGAGTTGTAATTGGCTGTGA
PRIMER_LEFT=179,22
PRIMER_RIGHT=444,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.0409
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=CACGTTCTCATGGTAATCGAAA
PRIMER_RIGHT_1_SEQUENCE=TGGAGTTGTAATTGGCTGTGAC
PRIMER_LEFT_1=179,22
PRIMER_RIGHT_1=443,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.0000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.0629
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=0.037339
PRIMER_LEFT_2_SEQUENCE=AGAGGCACGTTCTCATGGTAAT
PRIMER_RIGHT_2_SEQUENCE=GTGGAGTTGTAATTGGCTGTGA
PRIMER_LEFT_2=174,22
PRIMER_RIGHT_2=444,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=0.0629
PRIMER_LEFT_3_PENALTY=0.025566
PRIMER_RIGHT_3_PENALTY=0.037339
PRIMER_LEFT_3_SEQUENCE=AGAGGCACGTTCTCATGGTAAT
PRIMER_RIGHT_3_SEQUENCE=TGGAGTTGTAATTGGCTGTGAC
PRIMER_LEFT_3=174,22
PRIMER_RIGHT_3=443,22
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=60.037
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.0992
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.095574
PRIMER_LEFT_4_SEQUENCE=CACGTTCTCATGGTAATCGAAA
PRIMER_RIGHT_4_SEQUENCE=CATCCTTGAAGAGGCTGTATCC
PRIMER_LEFT_4=179,22
PRIMER_RIGHT_4=472,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.0000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_04260 Ps_contig_mira-and-tgicl-ass_franssen_32665
SEQUENCE=AAGGTGACCTTACGGAACATGTGTCGGGGNATTGTTCCATTTCCAACAACACAATCAATCTCTCTCTCTTGTTGTGTTACACAGAAATTCCAAACTCACTTTAGAATAGGAGAGAGAGAGTGACAGAGAAAAACCTTACTTACTTCCCTTAACTCTCTCTCTCTCTCTCTGATACAGCAAAGCATCATGATCTCCTCAACCAGATGCAACTTCCTGGGTAACTCGGGATTAGGTGGGTGTGGTAATGTCTGTGGTGGACAGAGAAGAGGTTCTGGACCTTGGAGGGTGAGCTGTATGTCACCAAGCAATGACAGAAATGGGAGCGTTGTTATGGAGACACCTTTGAAAGAAATTAAGAATGAGCCAGTGGTGGAAGATGTTGACAATAACCCGATCTCCGGTGGCGGTCCTCGCGATGTTTACGGCGAGGATAAGGCCACTGAGGATCATTCTGTTACTCCTTGGAGTGTTTCTGTTGCTAGTGGTTACACGTTGTTGCGAGATCCGCACTTCAACAAAGGGTTGGCATTCACTGAAAAAGAGAGGGATGCTCACTACTTGCGCGGTCTTCTTCCCCCAACAACTATTCCTCAAGAAACTCAGGTGAAGAAAATGATCCAGCACATACGCCAATATCAAGTTCCGTTGCAGCGATACACGGCAATGATGGATCTTCAGGAAAGAAACGAACGTCTGTTTTACAAGCTTCTGATTGAACACGTTGAAGAATTACTTCCGATTGTGTATACTCCAACTGTTGGTGAGGCTTGCCAGAAATACGGAGACATTTTTATGCGTCCACAGGGTCTTTATATAAGTTTGAACGAGAAAGGGAGGATTCTTGAAGTGCTGAGAAATTGGCCGGAGAAGAACATTCAAGTCATAGTCGTAACCGATGGAGAACGGATCTTAGGTCTTGGTGATCTAGGTTGTCAGGGGATGGGAATACCGGTGGGAAAACTTTCTTTATATACGGCACTTGGAGGAGTTCGTCCTTCAGCTTGCCTGCCTATAACGATTGATGTTGGGACAAACAATGAGAAGCTGCTCAATGATGAATTATATATCGGGCTAAAGCGAAGACGAGCAACCGGGCAAGAATATTCTGAACTCATACACGAGTTTATGACTGCAGTCAAGCAAACTTACGGCGAAAAAGTCCTAATTCAGTTTGAAGACTTTGCGAACCACAATGCTTTTGATTTGCTTGAAAAATATAGATCAACACATCTGGTTTTCAACGATGATATTCAGGGAACTGCATCAGTTGTTCTTGCTGGCTTAGTTGCAGCTTTGAAATTGGTTGGTGGAAACTTAGCTGACCACAGATTCTTATTCCTCGGTGCTGGAGAGGCTGGCACCGGAATCGCAGAACTCATTGCTCTTGAAATATCTAAACAGACAAATGGTCCGTTGGAGGAAATTCGCAAGAATATTTGGTTGGTGGACTCGAAGGGATTGATTGTTAAATCCCGAAAAGAATCACTCCAACATTTCAAGAAGCCGTGGGCTCATGAACACGAACCTGTTACAAGCCTTCTCGATGCTGTTAACCAAATTAAGCCAACAGTGCTTATCGGAACATCGGGACAAGGAAAAACTTTTACCAAAGAGGTGGTTGAGGCGATGGCTTCTCTTAATGAGAAACCGATTATTCTGGCTCTTTCCAACCCAACATCACAGTCAGAATGTACTGCTGAAGAAGCTTACTTGTGGAGCAGGGGAAGTGCGATATTTGCAAGTGGGAGCCCATTTCCGCCATTTGAATACAAGGGGAAAGTGTTTGTGCCTGGCCAGTCGAATAATGCGTACATATTCCCTGGGTTCGGCCTAGGTTTAATAATGTCCGGGACCATTCGCGTGCACGACGATCTTCTTCTCGCTGCATCTGAGGCATTGGCTGAACAAGTAACAGAAGAAAACTTTGAGAAGGGTCTGATATTTCCACCATTCACAAACATCAGAAAGATTTCAGCTCACATTGCTGCCAAAGTTGCTGCTAAGGCTTATGAGCTTGGTTTGGCGACTCGACTTCCTCAGCCGAAAGATTTGGAGAAGTTTGCAGAGAGTTGTATGTACACTCCGGCTTACAGAACCTACCGGTGAAGTGACAAAAGACAATAATACCCCATTTTCATGAAATTATTTTACTGTCAATTTTTTATTTATTTATATTTTATTTTTCTGTTTTTGTTTTTATAAGTAATAATTGAATTGGGTGGGTTAGGAAGGAAATTAGGAAGGTGTAGTTGTAGTTAAACTACTAAGGGTAGATTGAAAGTTTATGTTAATGATCTATATATACATATTTTTTCTAGTTTCTGTGTGACAGTATTTAAAATTAGAAATGGAACCATTTTTGAAAAAAAGTC
TARGET=59,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0783
PRIMER_LEFT_PENALTY=2.043808
PRIMER_RIGHT_PENALTY=0.034537
PRIMER_LEFT_SEQUENCE=GGGNATTGTTCCATTTCCAAC
PRIMER_RIGHT_SEQUENCE=TGTCATTGCTTGGTGACATACA
PRIMER_LEFT=26,21
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=61.044
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.50
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=2.1530
PRIMER_LEFT_1_PENALTY=2.043808
PRIMER_RIGHT_1_PENALTY=0.109200
PRIMER_LEFT_1_SEQUENCE=GGGNATTGTTCCATTTCCAAC
PRIMER_RIGHT_1_SEQUENCE=TCTGTCCACCACAGACATTACC
PRIMER_LEFT_1=26,21
PRIMER_RIGHT_1=261,22
PRIMER_LEFT_1_TM=61.044
PRIMER_RIGHT_1_TM=59.891
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.50
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.75
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=2.1992
PRIMER_LEFT_2_PENALTY=2.043808
PRIMER_RIGHT_2_PENALTY=0.155362
PRIMER_LEFT_2_SEQUENCE=GGGNATTGTTCCATTTCCAAC
PRIMER_RIGHT_2_SEQUENCE=GAACCTCTTCTCTGTCCACCAC
PRIMER_LEFT_2=26,21
PRIMER_RIGHT_2=271,22
PRIMER_LEFT_2_TM=61.044
PRIMER_RIGHT_2_TM=60.155
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.50
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=2.2573
PRIMER_LEFT_3_PENALTY=2.043808
PRIMER_RIGHT_3_PENALTY=0.213506
PRIMER_LEFT_3_SEQUENCE=GGGNATTGTTCCATTTCCAAC
PRIMER_RIGHT_3_SEQUENCE=TTGAGGAGATCATGATGCTTTG
PRIMER_LEFT_3=26,21
PRIMER_RIGHT_3=198,22
PRIMER_LEFT_3_TM=61.044
PRIMER_RIGHT_3_TM=60.214
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.50
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=173
PRIMER_PAIR_PENALTY_4=2.2698
PRIMER_LEFT_4_PENALTY=2.043808
PRIMER_RIGHT_4_PENALTY=0.225996
PRIMER_LEFT_4_SEQUENCE=GGGNATTGTTCCATTTCCAAC
PRIMER_RIGHT_4_SEQUENCE=CAGACATTACCACACCCACCTA
PRIMER_LEFT_4=26,21
PRIMER_RIGHT_4=250,22
PRIMER_LEFT_4_TM=61.044
PRIMER_RIGHT_4_TM=59.774
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.50
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_04261 Ps_contig_mira-and-tgicl-ass_franssen_32665
SEQUENCE=AAGGTGACCTTACGGAACATGTGTCGGGGNATTGTTCCATTTCCAACAACACAATCAATCTCTCTCTCTTGTTGTGTTACACAGAAATTCCAAACTCACTTTAGAATAGGAGAGAGAGAGTGACAGAGAAAAACCTTACTTACTTCCCTTAACTCTCTCTCTCTCTCTCTGATACAGCAAAGCATCATGATCTCCTCAACCAGATGCAACTTCCTGGGTAACTCGGGATTAGGTGGGTGTGGTAATGTCTGTGGTGGACAGAGAAGAGGTTCTGGACCTTGGAGGGTGAGCTGTATGTCACCAAGCAATGACAGAAATGGGAGCGTTGTTATGGAGACACCTTTGAAAGAAATTAAGAATGAGCCAGTGGTGGAAGATGTTGACAATAACCCGATCTCCGGTGGCGGTCCTCGCGATGTTTACGGCGAGGATAAGGCCACTGAGGATCATTCTGTTACTCCTTGGAGTGTTTCTGTTGCTAGTGGTTACACGTTGTTGCGAGATCCGCACTTCAACAAAGGGTTGGCATTCACTGAAAAAGAGAGGGATGCTCACTACTTGCGCGGTCTTCTTCCCCCAACAACTATTCCTCAAGAAACTCAGGTGAAGAAAATGATCCAGCACATACGCCAATATCAAGTTCCGTTGCAGCGATACACGGCAATGATGGATCTTCAGGAAAGAAACGAACGTCTGTTTTACAAGCTTCTGATTGAACACGTTGAAGAATTACTTCCGATTGTGTATACTCCAACTGTTGGTGAGGCTTGCCAGAAATACGGAGACATTTTTATGCGTCCACAGGGTCTTTATATAAGTTTGAACGAGAAAGGGAGGATTCTTGAAGTGCTGAGAAATTGGCCGGAGAAGAACATTCAAGTCATAGTCGTAACCGATGGAGAACGGATCTTAGGTCTTGGTGATCTAGGTTGTCAGGGGATGGGAATACCGGTGGGAAAACTTTCTTTATATACGGCACTTGGAGGAGTTCGTCCTTCAGCTTGCCTGCCTATAACGATTGATGTTGGGACAAACAATGAGAAGCTGCTCAATGATGAATTATATATCGGGCTAAAGCGAAGACGAGCAACCGGGCAAGAATATTCTGAACTCATACACGAGTTTATGACTGCAGTCAAGCAAACTTACGGCGAAAAAGTCCTAATTCAGTTTGAAGACTTTGCGAACCACAATGCTTTTGATTTGCTTGAAAAATATAGATCAACACATCTGGTTTTCAACGATGATATTCAGGGAACTGCATCAGTTGTTCTTGCTGGCTTAGTTGCAGCTTTGAAATTGGTTGGTGGAAACTTAGCTGACCACAGATTCTTATTCCTCGGTGCTGGAGAGGCTGGCACCGGAATCGCAGAACTCATTGCTCTTGAAATATCTAAACAGACAAATGGTCCGTTGGAGGAAATTCGCAAGAATATTTGGTTGGTGGACTCGAAGGGATTGATTGTTAAATCCCGAAAAGAATCACTCCAACATTTCAAGAAGCCGTGGGCTCATGAACACGAACCTGTTACAAGCCTTCTCGATGCTGTTAACCAAATTAAGCCAACAGTGCTTATCGGAACATCGGGACAAGGAAAAACTTTTACCAAAGAGGTGGTTGAGGCGATGGCTTCTCTTAATGAGAAACCGATTATTCTGGCTCTTTCCAACCCAACATCACAGTCAGAATGTACTGCTGAAGAAGCTTACTTGTGGAGCAGGGGAAGTGCGATATTTGCAAGTGGGAGCCCATTTCCGCCATTTGAATACAAGGGGAAAGTGTTTGTGCCTGGCCAGTCGAATAATGCGTACATATTCCCTGGGTTCGGCCTAGGTTTAATAATGTCCGGGACCATTCGCGTGCACGACGATCTTCTTCTCGCTGCATCTGAGGCATTGGCTGAACAAGTAACAGAAGAAAACTTTGAGAAGGGTCTGATATTTCCACCATTCACAAACATCAGAAAGATTTCAGCTCACATTGCTGCCAAAGTTGCTGCTAAGGCTTATGAGCTTGGTTTGGCGACTCGACTTCCTCAGCCGAAAGATTTGGAGAAGTTTGCAGAGAGTTGTATGTACACTCCGGCTTACAGAACCTACCGGTGAAGTGACAAAAGACAATAATACCCCATTTTCATGAAATTATTTTACTGTCAATTTTTTATTTATTTATATTTTATTTTTCTGTTTTTGTTTTTATAAGTAATAATTGAATTGGGTGGGTTAGGAAGGAAATTAGGAAGGTGTAGTTGTAGTTAAACTACTAAGGGTAGATTGAAAGTTTATGTTAATGATCTATATATACATATTTTTTCTAGTTTCTGTGTGACAGTATTTAAAATTAGAAATGGAACCATTTTTGAAAAAAAGTC
TARGET=110,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0744
PRIMER_LEFT_PENALTY=2.039879
PRIMER_RIGHT_PENALTY=0.034537
PRIMER_LEFT_SEQUENCE=CCAACAACACAATCAATCTCTCTC
PRIMER_RIGHT_SEQUENCE=TGTCATTGCTTGGTGACATACA
PRIMER_LEFT=42,24
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=2.0783
PRIMER_LEFT_1_PENALTY=2.043808
PRIMER_RIGHT_1_PENALTY=0.034537
PRIMER_LEFT_1_SEQUENCE=GGGNATTGTTCCATTTCCAAC
PRIMER_RIGHT_1_SEQUENCE=TGTCATTGCTTGGTGACATACA
PRIMER_LEFT_1=26,21
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=61.044
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.50
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=2.1416
PRIMER_LEFT_2_PENALTY=2.039879
PRIMER_RIGHT_2_PENALTY=0.101680
PRIMER_LEFT_2_SEQUENCE=CCAACAACACAATCAATCTCTCTC
PRIMER_RIGHT_2_SEQUENCE=ATAACAACGCTCCCATTTCTGT
PRIMER_LEFT_2=42,24
PRIMER_RIGHT_2=331,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=2.1491
PRIMER_LEFT_3_PENALTY=2.039879
PRIMER_RIGHT_3_PENALTY=0.109200
PRIMER_LEFT_3_SEQUENCE=CCAACAACACAATCAATCTCTCTC
PRIMER_RIGHT_3_SEQUENCE=TCTGTCCACCACAGACATTACC
PRIMER_LEFT_3=42,24
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=59.891
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=2.1530
PRIMER_LEFT_4_PENALTY=2.043808
PRIMER_RIGHT_4_PENALTY=0.109200
PRIMER_LEFT_4_SEQUENCE=GGGNATTGTTCCATTTCCAAC
PRIMER_RIGHT_4_SEQUENCE=TCTGTCCACCACAGACATTACC
PRIMER_LEFT_4=26,21
PRIMER_RIGHT_4=261,22
PRIMER_LEFT_4_TM=61.044
PRIMER_RIGHT_4_TM=59.891
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.50
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_04262 Ps_contig_mira-and-tgicl-ass_franssen_32665
SEQUENCE=AAGGTGACCTTACGGAACATGTGTCGGGGNATTGTTCCATTTCCAACAACACAATCAATCTCTCTCTCTTGTTGTGTTACACAGAAATTCCAAACTCACTTTAGAATAGGAGAGAGAGAGTGACAGAGAAAAACCTTACTTACTTCCCTTAACTCTCTCTCTCTCTCTCTGATACAGCAAAGCATCATGATCTCCTCAACCAGATGCAACTTCCTGGGTAACTCGGGATTAGGTGGGTGTGGTAATGTCTGTGGTGGACAGAGAAGAGGTTCTGGACCTTGGAGGGTGAGCTGTATGTCACCAAGCAATGACAGAAATGGGAGCGTTGTTATGGAGACACCTTTGAAAGAAATTAAGAATGAGCCAGTGGTGGAAGATGTTGACAATAACCCGATCTCCGGTGGCGGTCCTCGCGATGTTTACGGCGAGGATAAGGCCACTGAGGATCATTCTGTTACTCCTTGGAGTGTTTCTGTTGCTAGTGGTTACACGTTGTTGCGAGATCCGCACTTCAACAAAGGGTTGGCATTCACTGAAAAAGAGAGGGATGCTCACTACTTGCGCGGTCTTCTTCCCCCAACAACTATTCCTCAAGAAACTCAGGTGAAGAAAATGATCCAGCACATACGCCAATATCAAGTTCCGTTGCAGCGATACACGGCAATGATGGATCTTCAGGAAAGAAACGAACGTCTGTTTTACAAGCTTCTGATTGAACACGTTGAAGAATTACTTCCGATTGTGTATACTCCAACTGTTGGTGAGGCTTGCCAGAAATACGGAGACATTTTTATGCGTCCACAGGGTCTTTATATAAGTTTGAACGAGAAAGGGAGGATTCTTGAAGTGCTGAGAAATTGGCCGGAGAAGAACATTCAAGTCATAGTCGTAACCGATGGAGAACGGATCTTAGGTCTTGGTGATCTAGGTTGTCAGGGGATGGGAATACCGGTGGGAAAACTTTCTTTATATACGGCACTTGGAGGAGTTCGTCCTTCAGCTTGCCTGCCTATAACGATTGATGTTGGGACAAACAATGAGAAGCTGCTCAATGATGAATTATATATCGGGCTAAAGCGAAGACGAGCAACCGGGCAAGAATATTCTGAACTCATACACGAGTTTATGACTGCAGTCAAGCAAACTTACGGCGAAAAAGTCCTAATTCAGTTTGAAGACTTTGCGAACCACAATGCTTTTGATTTGCTTGAAAAATATAGATCAACACATCTGGTTTTCAACGATGATATTCAGGGAACTGCATCAGTTGTTCTTGCTGGCTTAGTTGCAGCTTTGAAATTGGTTGGTGGAAACTTAGCTGACCACAGATTCTTATTCCTCGGTGCTGGAGAGGCTGGCACCGGAATCGCAGAACTCATTGCTCTTGAAATATCTAAACAGACAAATGGTCCGTTGGAGGAAATTCGCAAGAATATTTGGTTGGTGGACTCGAAGGGATTGATTGTTAAATCCCGAAAAGAATCACTCCAACATTTCAAGAAGCCGTGGGCTCATGAACACGAACCTGTTACAAGCCTTCTCGATGCTGTTAACCAAATTAAGCCAACAGTGCTTATCGGAACATCGGGACAAGGAAAAACTTTTACCAAAGAGGTGGTTGAGGCGATGGCTTCTCTTAATGAGAAACCGATTATTCTGGCTCTTTCCAACCCAACATCACAGTCAGAATGTACTGCTGAAGAAGCTTACTTGTGGAGCAGGGGAAGTGCGATATTTGCAAGTGGGAGCCCATTTCCGCCATTTGAATACAAGGGGAAAGTGTTTGTGCCTGGCCAGTCGAATAATGCGTACATATTCCCTGGGTTCGGCCTAGGTTTAATAATGTCCGGGACCATTCGCGTGCACGACGATCTTCTTCTCGCTGCATCTGAGGCATTGGCTGAACAAGTAACAGAAGAAAACTTTGAGAAGGGTCTGATATTTCCACCATTCACAAACATCAGAAAGATTTCAGCTCACATTGCTGCCAAAGTTGCTGCTAAGGCTTATGAGCTTGGTTTGGCGACTCGACTTCCTCAGCCGAAAGATTTGGAGAAGTTTGCAGAGAGTTGTATGTACACTCCGGCTTACAGAACCTACCGGTGAAGTGACAAAAGACAATAATACCCCATTTTCATGAAATTATTTTACTGTCAATTTTTTATTTATTTATATTTTATTTTTCTGTTTTTGTTTTTATAAGTAATAATTGAATTGGGTGGGTTAGGAAGGAAATTAGGAAGGTGTAGTTGTAGTTAAACTACTAAGGGTAGATTGAAAGTTTATGTTAATGATCTATATATACATATTTTTTCTAGTTTCTGTGTGACAGTATTTAAAATTAGAAATGGAACCATTTTTGAAAAAAAGTC
TARGET=153,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0744
PRIMER_LEFT_PENALTY=2.039879
PRIMER_RIGHT_PENALTY=0.034537
PRIMER_LEFT_SEQUENCE=CCAACAACACAATCAATCTCTCTC
PRIMER_RIGHT_SEQUENCE=TGTCATTGCTTGGTGACATACA
PRIMER_LEFT=42,24
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=2.0783
PRIMER_LEFT_1_PENALTY=2.043808
PRIMER_RIGHT_1_PENALTY=0.034537
PRIMER_LEFT_1_SEQUENCE=GGGNATTGTTCCATTTCCAAC
PRIMER_RIGHT_1_SEQUENCE=TGTCATTGCTTGGTGACATACA
PRIMER_LEFT_1=26,21
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=61.044
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.50
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=2.1416
PRIMER_LEFT_2_PENALTY=2.039879
PRIMER_RIGHT_2_PENALTY=0.101680
PRIMER_LEFT_2_SEQUENCE=CCAACAACACAATCAATCTCTCTC
PRIMER_RIGHT_2_SEQUENCE=ATAACAACGCTCCCATTTCTGT
PRIMER_LEFT_2=42,24
PRIMER_RIGHT_2=331,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=2.1491
PRIMER_LEFT_3_PENALTY=2.039879
PRIMER_RIGHT_3_PENALTY=0.109200
PRIMER_LEFT_3_SEQUENCE=CCAACAACACAATCAATCTCTCTC
PRIMER_RIGHT_3_SEQUENCE=TCTGTCCACCACAGACATTACC
PRIMER_LEFT_3=42,24
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=59.891
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=2.1530
PRIMER_LEFT_4_PENALTY=2.043808
PRIMER_RIGHT_4_PENALTY=0.109200
PRIMER_LEFT_4_SEQUENCE=GGGNATTGTTCCATTTCCAAC
PRIMER_RIGHT_4_SEQUENCE=TCTGTCCACCACAGACATTACC
PRIMER_LEFT_4=26,21
PRIMER_RIGHT_4=261,22
PRIMER_LEFT_4_TM=61.044
PRIMER_RIGHT_4_TM=59.891
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.50
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_04263 Ps_contig_mira-and-tgicl-ass_franssen_32665
SEQUENCE=AAGGTGACCTTACGGAACATGTGTCGGGGNATTGTTCCATTTCCAACAACACAATCAATCTCTCTCTCTTGTTGTGTTACACAGAAATTCCAAACTCACTTTAGAATAGGAGAGAGAGAGTGACAGAGAAAAACCTTACTTACTTCCCTTAACTCTCTCTCTCTCTCTCTGATACAGCAAAGCATCATGATCTCCTCAACCAGATGCAACTTCCTGGGTAACTCGGGATTAGGTGGGTGTGGTAATGTCTGTGGTGGACAGAGAAGAGGTTCTGGACCTTGGAGGGTGAGCTGTATGTCACCAAGCAATGACAGAAATGGGAGCGTTGTTATGGAGACACCTTTGAAAGAAATTAAGAATGAGCCAGTGGTGGAAGATGTTGACAATAACCCGATCTCCGGTGGCGGTCCTCGCGATGTTTACGGCGAGGATAAGGCCACTGAGGATCATTCTGTTACTCCTTGGAGTGTTTCTGTTGCTAGTGGTTACACGTTGTTGCGAGATCCGCACTTCAACAAAGGGTTGGCATTCACTGAAAAAGAGAGGGATGCTCACTACTTGCGCGGTCTTCTTCCCCCAACAACTATTCCTCAAGAAACTCAGGTGAAGAAAATGATCCAGCACATACGCCAATATCAAGTTCCGTTGCAGCGATACACGGCAATGATGGATCTTCAGGAAAGAAACGAACGTCTGTTTTACAAGCTTCTGATTGAACACGTTGAAGAATTACTTCCGATTGTGTATACTCCAACTGTTGGTGAGGCTTGCCAGAAATACGGAGACATTTTTATGCGTCCACAGGGTCTTTATATAAGTTTGAACGAGAAAGGGAGGATTCTTGAAGTGCTGAGAAATTGGCCGGAGAAGAACATTCAAGTCATAGTCGTAACCGATGGAGAACGGATCTTAGGTCTTGGTGATCTAGGTTGTCAGGGGATGGGAATACCGGTGGGAAAACTTTCTTTATATACGGCACTTGGAGGAGTTCGTCCTTCAGCTTGCCTGCCTATAACGATTGATGTTGGGACAAACAATGAGAAGCTGCTCAATGATGAATTATATATCGGGCTAAAGCGAAGACGAGCAACCGGGCAAGAATATTCTGAACTCATACACGAGTTTATGACTGCAGTCAAGCAAACTTACGGCGAAAAAGTCCTAATTCAGTTTGAAGACTTTGCGAACCACAATGCTTTTGATTTGCTTGAAAAATATAGATCAACACATCTGGTTTTCAACGATGATATTCAGGGAACTGCATCAGTTGTTCTTGCTGGCTTAGTTGCAGCTTTGAAATTGGTTGGTGGAAACTTAGCTGACCACAGATTCTTATTCCTCGGTGCTGGAGAGGCTGGCACCGGAATCGCAGAACTCATTGCTCTTGAAATATCTAAACAGACAAATGGTCCGTTGGAGGAAATTCGCAAGAATATTTGGTTGGTGGACTCGAAGGGATTGATTGTTAAATCCCGAAAAGAATCACTCCAACATTTCAAGAAGCCGTGGGCTCATGAACACGAACCTGTTACAAGCCTTCTCGATGCTGTTAACCAAATTAAGCCAACAGTGCTTATCGGAACATCGGGACAAGGAAAAACTTTTACCAAAGAGGTGGTTGAGGCGATGGCTTCTCTTAATGAGAAACCGATTATTCTGGCTCTTTCCAACCCAACATCACAGTCAGAATGTACTGCTGAAGAAGCTTACTTGTGGAGCAGGGGAAGTGCGATATTTGCAAGTGGGAGCCCATTTCCGCCATTTGAATACAAGGGGAAAGTGTTTGTGCCTGGCCAGTCGAATAATGCGTACATATTCCCTGGGTTCGGCCTAGGTTTAATAATGTCCGGGACCATTCGCGTGCACGACGATCTTCTTCTCGCTGCATCTGAGGCATTGGCTGAACAAGTAACAGAAGAAAACTTTGAGAAGGGTCTGATATTTCCACCATTCACAAACATCAGAAAGATTTCAGCTCACATTGCTGCCAAAGTTGCTGCTAAGGCTTATGAGCTTGGTTTGGCGACTCGACTTCCTCAGCCGAAAGATTTGGAGAAGTTTGCAGAGAGTTGTATGTACACTCCGGCTTACAGAACCTACCGGTGAAGTGACAAAAGACAATAATACCCCATTTTCATGAAATTATTTTACTGTCAATTTTTTATTTATTTATATTTTATTTTTCTGTTTTTGTTTTTATAAGTAATAATTGAATTGGGTGGGTTAGGAAGGAAATTAGGAAGGTGTAGTTGTAGTTAAACTACTAAGGGTAGATTGAAAGTTTATGTTAATGATCTATATATACATATTTTTTCTAGTTTCTGTGTGACAGTATTTAAAATTAGAAATGGAACCATTTTTGAAAAAAAGTC
TARGET=2161,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4591
PRIMER_LEFT_PENALTY=0.060824
PRIMER_RIGHT_PENALTY=0.398258
PRIMER_LEFT_SEQUENCE=GTACACTCCGGCTTACAGAACC
PRIMER_RIGHT_SEQUENCE=TAATTTCCTTCCTAACCCACCC
PRIMER_LEFT=2075,22
PRIMER_RIGHT=2237,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=60.398
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.5004
PRIMER_LEFT_1_PENALTY=0.102139
PRIMER_RIGHT_1_PENALTY=0.398258
PRIMER_LEFT_1_SEQUENCE=CCGAAAGATTTGGAGAAGTTTG
PRIMER_RIGHT_1_SEQUENCE=TAATTTCCTTCCTAACCCACCC
PRIMER_LEFT_1=2040,22
PRIMER_RIGHT_1=2237,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.398
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.5833
PRIMER_LEFT_2_PENALTY=0.185055
PRIMER_RIGHT_2_PENALTY=0.398258
PRIMER_LEFT_2_SEQUENCE=CACTCCGGCTTACAGAACCTAC
PRIMER_RIGHT_2_SEQUENCE=TAATTTCCTTCCTAACCCACCC
PRIMER_LEFT_2=2078,22
PRIMER_RIGHT_2=2237,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=60.398
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=0.5833
PRIMER_LEFT_3_PENALTY=0.185055
PRIMER_RIGHT_3_PENALTY=0.398258
PRIMER_LEFT_3_SEQUENCE=TACACTCCGGCTTACAGAACCT
PRIMER_RIGHT_3_SEQUENCE=TAATTTCCTTCCTAACCCACCC
PRIMER_LEFT_3=2076,22
PRIMER_RIGHT_3=2237,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=60.398
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=0.5833
PRIMER_LEFT_4_PENALTY=0.185055
PRIMER_RIGHT_4_PENALTY=0.398258
PRIMER_LEFT_4_SEQUENCE=ACACTCCGGCTTACAGAACCTA
PRIMER_RIGHT_4_SEQUENCE=TAATTTCCTTCCTAACCCACCC
PRIMER_LEFT_4=2077,22
PRIMER_RIGHT_4=2237,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=60.398
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_04264 Ps_contig_mira-and-tgicl-ass_franssen_32666
SEQUENCE=GGTATCAACGCAGAGTAGRRRNGGAAGATCCAAATTTCCCATAACTCATCATCCAGAGAGAGAAAGAGGAGAGAGAAAGTACTCTCAATTTTACCATCGAGGACTCTAAAAAAACGAAACTGAATCTCAGAAGAGAGAGAGAAAATCATTGTTGAGAAGAAACGAAGCTTCGTGTAACTACATTAATGGCGGAAACGAAGGAAGAGAGTAAATCTAACGAAGAGAGTGTGAAACTTTTCGTAGGTCAAGTACCGAAGCACATGACGGAAGATGAGTTACTTACAATGTTCAAAGAGTTTGCTCTGGTTGAAGAAGTCAACATCATCAAAGACAAAGCCACTCGCGCTTCTCGAGGTTGTTGCTTTGTGATTTGTCCTTCTAGAGATGAAGCTGATAAGGCCGTTAATGCGTGTCACAATAAGAAAACTTTACCTGGGGCATCTAGTCCTTTGCAAGTGAAGTATGCTGACGGTGAGCTGGAAAGATTAGAACACAAACTTTTCATTGGCATGCTTCCAAAGAATGTTTCTGAAGTTGAAGTATCCGAACTTTTTTCCAAGTACGGAACTATAAAGGATTTACAGCTTTTAAGAGGTTCTCAACAGACAAGTAAAGTGGTTGTGCATTTTTGAAATATGAAACTAAAGAACAAGCTCTTGCTGCTTTGGAGGCAATCAATGGAAAACACAAAATGGAGGGTTCAAGTGTTCCTTTAGTTGTCAAATGGGCTGATACTGAAAAGGAAAGACAAGCCCGAAGAGCTCAGAAAGCACAATCTCAAGCTTCTAATGTGGCACATGCTGACTCTCAGCATCCTTCCTTGTTTGGAGCCATGCCTATGGGCTACGTTCCCCCATATAATGGCTATGGTTATCAGGCTCCAGCAAGTTATGGGCTCATGCCGTACCGCATGCCGCCAATGCAGAATCAAGCTGGTTACCATAATATGATGCCTCACATGAATCAAGGAAATGCTTTGCGGCCTGATCTTGGCCCCAACATGAATCCTAGAAACTATCATGTGCCTCCTGCAAGTTATGTTGGTTCTTATCCTGCTGTGCCAGGTCTACAACCTCCAATGCCCTATCCTGCAGGTATGATTAGTCCAAGGCCTATGAATAGTCCTCCCGGTTCTGTTTCGCCTTCCGGTGGAAACGGTAACTCAGCTACATCTTCAGGGTCCAGCAAAAATTCAGGCGGTGGTCAGATTGAAGGACCACCTGGCGCAAATCTATTTATCTACCACATACCTCAAGAATTTGGAGATCAAGAGCTTGCTAATGCTTTTCAACCTTTTGGTAGAGTTTTAAGCGCTAAAGTTTTTGTTGACAAAGCAACCGGTGTCAGCAAATGTTTTGGGTTTGTAAGTTATGATTCCCCAGATCATGCTCAATCTGCAATTAGTATGATGAATGGATGCCAATTAGGAGGTAAGAAATTAAAGGTTCAGCATAAGAGGGACAACAAACCCGGTAAACCTTATTGATGAAGATGAACAATGGAGTTTTGTATAATTTACATTGTAATTCATGACCAGTCTAATGTTAATACAATTTGTAATTCCTGTAACATGAAAGTTTATTTAGACATTGATTAATTTATTGATTTTTCTACCCAAGAAAAGT
TARGET=132,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04265 Ps_contig_mira-and-tgicl-ass_franssen_32671
SEQUENCE=AAAGGCAGTCATCTCTAGGATGCTCCATTATTCTGCATAATAACCGCCTTAGAATTGAACCAACGCGAAATTGAGAAGCGAAAATGGGATGGGTTTGGAGCGACGACAACGACGACGACGATTCTCAACGCAACATCTCTTCAGGCTCCGATGAACATAGCTCCACCAGGAAAATCGTGAAATCGCAGTGCAGAACGGAAGAGGTTGAACCTGGAAAGTTCCTTAGGAAATGCGAGAAAACTGAAGAGCTTCTCAGAACTTGCGCTGGAAGGCCTGTTGAAGTGCTGCAATCAAACAAGGAGTACACGGAAGAAGATATCACTAATGAGGTACTTAGAGCAAGATCTACCACATCTGGCTCATCAAACAGCTCACCAGACCATGGTGTGTTCTTCCCTGGGCTACAAAATGATATTGAAAACATGGAACGCAGCTTCTTCTTTGGTGGTATCAGTCGCTTATTTGAAGCAGCTGAAGAAATGAAAAATGGCCTTTTTGATGTTATTGCAAAGGCAGAGTCGTCATCTTCATCCCCTACGAGGAGAGGGATACCTATTGAAGAGTATGGCCGACAAGAAACTCGTCCTAGATCCATGGATATGGATTCAGGGGATACTGATTTCACTGGATTGGCGAAGGACGTTTAAACTAAAGTGTTTTCCATACTTGCTATCATTTTTGAGTTTGATCTCGAACATTTCGACTACATATATGCCCTTTAATAAGGTCTTTTTTCCCTGCTGTTTAGTTTTTGTTTCTTATTAAGTAAA
TARGET=109,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5741
PRIMER_LEFT_PENALTY=0.546410
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=CAGTCATCTCTAGGATGCTCCA
PRIMER_RIGHT_SEQUENCE=GCTCTTCAGTTTTCTCGCATTT
PRIMER_LEFT=5,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=59.454
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.6542
PRIMER_LEFT_1_PENALTY=0.546410
PRIMER_RIGHT_1_PENALTY=0.107763
PRIMER_LEFT_1_SEQUENCE=CAGTCATCTCTAGGATGCTCCA
PRIMER_RIGHT_1_SEQUENCE=CTTCAGTTTTCTCGCATTTCCT
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=245,22
PRIMER_LEFT_1_TM=59.454
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.8436
PRIMER_LEFT_2_PENALTY=0.815922
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=TAACCGCCTTAGAATTGAACCA
PRIMER_RIGHT_2_SEQUENCE=GCTCTTCAGTTTTCTCGCATTT
PRIMER_LEFT_2=40,22
PRIMER_RIGHT_2=248,22
PRIMER_LEFT_2_TM=60.816
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.8668
PRIMER_LEFT_3_PENALTY=0.546410
PRIMER_RIGHT_3_PENALTY=0.320415
PRIMER_LEFT_3_SEQUENCE=CAGTCATCTCTAGGATGCTCCA
PRIMER_RIGHT_3_SEQUENCE=AGCTCTTCAGTTTTCTCGCATT
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=59.454
PRIMER_RIGHT_3_TM=59.680
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.8668
PRIMER_LEFT_4_PENALTY=0.546410
PRIMER_RIGHT_4_PENALTY=0.320415
PRIMER_LEFT_4_SEQUENCE=CAGTCATCTCTAGGATGCTCCA
PRIMER_RIGHT_4_SEQUENCE=AAGCTCTTCAGTTTTCTCGCAT
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=250,22
PRIMER_LEFT_4_TM=59.454
PRIMER_RIGHT_4_TM=59.680
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=10.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_04266 Ps_contig_mira-and-tgicl-ass_franssen_32676
SEQUENCE=TAGCTTATAAAAACGCATAATCTATGTTAGAACAGTTTGAATTTATTTTATCCATAGTGTTTAATTAAGTTATATATTCAAACATGGCCAAACTCAACTAACAGCTACGTTGTACAGAAAACAAAATGTCCTTTGATTTTCACCCTGCCAACATCTTCAACAAAACAATGATTGATGTAACATCATCATATATTCTGCATACATTTCAACCACACAATTTTACTTGTTCTGTTTCACTCAAGACCAAAAGTCCCACCATTGATAATCACAACTTGATTGGCCTAACAGATCATTTGATTCAAAACCACTCCAATTTTCTGGTGAAGTTAATGATCCATCACCATGTTCAGCAAAACTCATAAGTCCATGTTCATCTTCAAGCCCAAAATATTCAACTTTTATGCTTGTGTCATCATCTGATACAACATCAAGAACATGTTCTGATCTTTCCATCGAAGTACTTGGTTTCACCTCAGCCTCACATTTGATTGTTCCTCCATTTTCTGATTCACTTTCCATGCTGTTCGCTTCTTTAACTTGTGATGAACTCTGACTTTGCTCTATTGGCTTCTGTATTAGATCATTCAGCTTCTGCAACTGTATGAGTAATGTTTGTTTTTCCTTCTTCATTGATTCAAATCTTGAAGCCAAACTATTATAACTATTTTGAAGTTTATTATACTCTCTTTCAAGTTGCTTTGATTTCCATCTAGCTCTCTTATTTTGAAACCATATAGCAACTTGTCTTGGCTGCAACCCAAGCTCTCTTGCCAACTGCAGCTTCTTTCTTGGTTCAAGTCTTGTCTCAGTCTCAAACATGGTTTCCAATGATTTGATTTGTTCATCACTAAACCTTTTTGTATTCTTGTTTTTCTTTCTTTCTTCATCATGGAAGGAATGTGCTGCTAGTTTGTCATAAGCTTCTTCCTCTGCTGCTGCTTCTTGCTGAATAAGCTACTGTATTGACCATACTCCATAGTTTCTTAATTACAAACAATGTTGTCTTAATTACACTTTGTAAGAT
TARGET=871,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1666
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.158297
PRIMER_LEFT_SEQUENCE=CAGTCTCAAACATGGTTTCCAA
PRIMER_RIGHT_SEQUENCE=ACTAGCAGCACATTCCTTCCAT
PRIMER_LEFT=806,22
PRIMER_RIGHT=909,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.158
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=0.2191
PRIMER_LEFT_1_PENALTY=0.060824
PRIMER_RIGHT_1_PENALTY=0.158297
PRIMER_LEFT_1_SEQUENCE=ACTGCAGCTTCTTTCTTGGTTC
PRIMER_RIGHT_1_SEQUENCE=ACTAGCAGCACATTCCTTCCAT
PRIMER_LEFT_1=773,22
PRIMER_RIGHT_1=909,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=60.158
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=0.2191
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.210886
PRIMER_LEFT_2_SEQUENCE=CAGTCTCAAACATGGTTTCCAA
PRIMER_RIGHT_2_SEQUENCE=AAACTAGCAGCACATTCCTTCC
PRIMER_LEFT_2=806,22
PRIMER_RIGHT_2=911,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=59.789
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=106
PRIMER_PAIR_PENALTY_3=0.2717
PRIMER_LEFT_3_PENALTY=0.060824
PRIMER_RIGHT_3_PENALTY=0.210886
PRIMER_LEFT_3_SEQUENCE=ACTGCAGCTTCTTTCTTGGTTC
PRIMER_RIGHT_3_SEQUENCE=AAACTAGCAGCACATTCCTTCC
PRIMER_LEFT_3=773,22
PRIMER_RIGHT_3=911,22
PRIMER_LEFT_3_TM=60.061
PRIMER_RIGHT_3_TM=59.789
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=0.3212
PRIMER_LEFT_4_PENALTY=0.008257
PRIMER_RIGHT_4_PENALTY=0.312895
PRIMER_LEFT_4_SEQUENCE=CAGTCTCAAACATGGTTTCCAA
PRIMER_RIGHT_4_SEQUENCE=TGACAAACTAGCAGCACATTCC
PRIMER_LEFT_4=806,22
PRIMER_RIGHT_4=915,22
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=60.313
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_04267 Ps_contig_mira-and-tgicl-ass_franssen_32678
SEQUENCE=AACGCAGAGTACGCGGGGGGAACGNAGRCAAAACCCTCTTGATATTTTCTTCTTCTCAGAACTTCACACAGAAAACTAAAAGTAGCAGAGACTCATCGAAGAAGAAGAAACAACCACATCTTCAAAATCATCAATCGTTCAACAAAATGAGTAGCATCGGAACAGGTTACGATCTATCCGTCACTACTTTCTCTCCCGACGGTCGCGTTTTCCAGATCGAGTACGCTGCTAAAGCCGTCGATAACAGCGGGACTGTTATTGGGATCAAATGCAAAGATGGAATTGTGTTGGGTGTTGAGAAGCTTATTCCTTCAAAAATGATGCTCCCGGGTTCCAATAGAAGAATACACGCGGTTCATCGCCACTCTGGAATGGCTGTAGCAGGATTAGCTGCAGACGGCAGGCAGATCGTTGCCAGGGCTAAATCTGAAGCAAATAACTATGACAGTGTGTACGGTGACCCAATTCCTGTTAAGGAGCTTGCCGATCGCGTGGCTAGCTATGTGCACCTTTGTACATTATATTGGTGGCTCAGACCTTTTGGCTGTGGTGTTATACTAGGTGGTTACGACAGAGATGGACCACAGTTGTACATGGTTGAACCTTCTGGTGTCTCTTATAGGTACTTCGGTGCTGCAATTGGGAAGGGCAGACAGGCTGCAAAAACAGAGATTGAAAAGTTGAAGCTTGCAGATTTGACCTGCCGACAGGGAGTTATTGAAGTAGCTAAGATTATATATGGAGTTCATGATGAGGCCAAGGATAAAGATTTCGAACTCGAAATGAGCTGGGTGTGTGAGGAATCAAATCGTCAACATGAAAAGGTCCCAGAAGAGCTTCTAGAAGAAGCTAAGACAGCAGCCAAAGCAGCTCTAGAAGAAATGGATGCAGATTAACAGATTAAAACTATGTATCCCAAAGATGTATTTCATTAACTTGTAATAGAGCATTGAGAGCATCTATAGAAAATTCATAGGCTTTTAATTTGAAGACTGCTCTGAAAAATTGGATTCTTGTATGCTTCTTGATGATTTCTGTTGCTGTGGAGAACTGTTTCAAAGTGCTTTAAAGACTGCTCTTATCAATTAATTTAGTGTTGAATTCTATCCGGCTTGGCCACTCTGGTTTGACAAAATGTTGTATGAAACCAAACATATCTTAATTCTAAACTT
TARGET=98,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04268 Ps_contig_mira-and-tgicl-ass_franssen_32687
SEQUENCE=ACGATAACATTGTTGAAAAAACATAAGGCATGAGAAATTTGTAGATAGATAAAATAATGAAAGTGTATTAACTGCTTCTGATAATGGCTACAACTATCAGATTCAAACCATTGTTTCCACTATCTCCTTTGCCTAAACAAACCAATTCACCTCTAACTTCATGGCATTCCCTTCCAGTTTCTCGTCCTTTCTTTCATCAACGTGGATTTGTTGCAGTTTCTGTTGCCTTCAACCCACAAGGGAACTTCGACGATGTCTCATTATTTGCTGATGATGATGAATCTAAAGTTGAACCTCCTATGCCTCCAAGTGAAGGCAGATTCAATGTTGTAATTGATAATGATGCTGTCAGTAGTCTTGACTTGTCCCCTTTTCAAGCTGCAACCGGCATGAAAAATCCTTTATCTGTGAAACCGCAAGAGTTTCTTGAGCGTTCCATTGGCTTTACCATCAACTATACAAGACCAGATCCTCGCGATCCTCGCGAGTTATCGGAGTATCCGGATATTAGACTATGGTTTGTGAGACTTGATGCTGCTTATCCATGGTTGCCGGTTTTGTTGGATTGGAGAGCTGGCGAGCTAGCTCGGTATGCTGCTATGTTAGTTCCACATCAGATGAATATGAAAATGGGGGTTGTATTTAATCCAGAAGCATTGGAATTGTTTGTTATGAATAAAGTATTTATTGTGTATTCATGGTTGAAGCATCACAACATTCCCAAGCCTGAATCAAAGGCAAATGATATGGCAAGAATGCTTGGATTTGGAATTGGATATGAACTATATGACTTAGTTGAAAATCATCCACTTCAAGAGTAAACAGTTTGTGACAATGATTGAGATTGAGTTTTTGAAACTTGATCATAAAAGTAATGTCATGCTAAGGATTTGTTTCTTCAAGATATTTAACTAGTGTTTCATATTTTGTATTCACACTTTTGGCAAATTTGACAGGGAAAATCTCTCTTTAATCATGTATTTTTTTCTATTTTTTTTATTATCTTATTATATTGATAAGT
TARGET=269,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1621
PRIMER_LEFT_PENALTY=0.102789
PRIMER_RIGHT_PENALTY=0.059316
PRIMER_LEFT_SEQUENCE=TCGACGATGTCTCATTATTTGC
PRIMER_RIGHT_SEQUENCE=AAGCAGCATCAAGTCTCACAAA
PRIMER_LEFT=246,22
PRIMER_RIGHT=539,22
PRIMER_LEFT_TM=60.103
PRIMER_RIGHT_TM=60.059
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=0.1710
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.136881
PRIMER_LEFT_1_SEQUENCE=CTAACTTCATGGCATTCCCTTC
PRIMER_RIGHT_1_SEQUENCE=CTTGAAAAGGGGACAAGTCAAG
PRIMER_LEFT_1=152,22
PRIMER_RIGHT_1=377,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.137
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.2059
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.171757
PRIMER_LEFT_2_SEQUENCE=CTAACTTCATGGCATTCCCTTC
PRIMER_RIGHT_2_SEQUENCE=AAACTCTTGCGGTTTCACAGAT
PRIMER_LEFT_2=152,22
PRIMER_RIGHT_2=424,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=60.172
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.2059
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.171757
PRIMER_LEFT_3_SEQUENCE=TCTAACTTCATGGCATTCCCTT
PRIMER_RIGHT_3_SEQUENCE=AAACTCTTGCGGTTTCACAGAT
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=424,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=60.172
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.2397
PRIMER_LEFT_4_PENALTY=0.102789
PRIMER_RIGHT_4_PENALTY=0.136881
PRIMER_LEFT_4_SEQUENCE=TCGACGATGTCTCATTATTTGC
PRIMER_RIGHT_4_SEQUENCE=CTTGAAAAGGGGACAAGTCAAG
PRIMER_LEFT_4=246,22
PRIMER_RIGHT_4=377,22
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=60.137
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_04269 Ps_contig_mira-and-tgicl-ass_franssen_32688
SEQUENCE=ACATATCATCATTACATTCTTTGCCTTCTCACACATTCTCTCTCTCTCTCTCCAACTCTTTTCTCTTCTGTCACTTTGAACACAGCACAACAAAAATTACAAAACCTAATTCGGTTTAATTCGCTCTTATTTCTATTTACTCATATCATAATGCAGACAAGGACGGTTCAAGTGAAGCAACTTTCAGATCTAGCTACTGAGAGAGAGATTTACGAATTCTTCTCATTTTCTGGAGAAATTGAAAACATTCAGATCGTAAGAGAATATGGAAAACCAAAGACTGCGTTTGTGACATTTAAAGATCCAAAAGCACTTGAAATTGCGTTGTTGTTATCGGGAGCAACAATAGTAGACCAGATTGTGAGTATAAGTCCAGTTGAGAACTATGTTGCAAATCGTGAGATGCAAGAAGCAAGGGCAGGGGAGTATTCTATAACTATTGCTCCTTCTGAAGATGCCGCATTGAATTCCGAGGAGGAGAAACCCGGCGGAAGAGTATATCTTAGTAAAGCACAAGATGTAGTTACGAATATGCTGGCAAAAGGATCAGCTATAAAACAAGATGCCGTGAACAAAGCTAAAGCATTTGATGAGAAGCATCAATTGACTGCCAATGCATCAGCAAAGGTCAGTTCATTTGACAAGAGAGTTGGACTGACCGAGAAATTGACAGTTGGACTCTCTGTAGTGAATGAGAAAGTGAAATCTGTGGATCAGAGGCTACATGTTTCAGATAAAACAATGGCAGCAATATTTGCAGCCGAGAGGAAGTTGAACGATACTGGATCAGCTGTCAAAACAAGCAGATACGTGACTGCCGGAACATCATGGCTAACGGGTGCTTTTAGCAAGGTGGCGAAAGCAGGAAACGTGGCCGGTACTAAAACTAGAGAAAAGTTTAACATGGCTGTTTCAAACTTGACAACAAAGGATACCTCATTGGTTGTATAGGCCAGAATTCAGAATTTCGTTACGACGGTCACTTCATACGGTCTCAAGTGAAGATCTGTTTCAGAGCTGTTGGTGTGTTAAAAAGGTTTGGTCTTTGAGATCGATCATGTACTTGTTTCTTTCTCTCCTTCAACAGTTCAATGTTTGTAGTAGTTAGCAACTATAGTGATTCAATTCAATCCAATTTTTTGTATTCTTTTATCACTCTGGTGTTTTTTAGCTGTTGCTTCTATGTAATTGGTGATTTTGGCTTTTTTTTCTTCTTTACGTTTTGTCTATAGGAAGTTATAGCTGTTGCTTCTATGTAGAAGATAACATCTTGTTTTTGTTATCAACACTTGAAGGATTCATTTGGGAAATTTTTATCTTCTAGATTACTACCAACCGTGGAATTNGTATAGATATTTTTTTANTTNGTNATANTGATATTTAAG
TARGET=37,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4253
PRIMER_LEFT_PENALTY=0.219884
PRIMER_RIGHT_PENALTY=0.205455
PRIMER_LEFT_SEQUENCE=ACATTCTTTGCCTTCTCACACA
PRIMER_RIGHT_SEQUENCE=AAAGTTGCTTCACTTGAACCGT
PRIMER_LEFT=13,22
PRIMER_RIGHT=183,22
PRIMER_LEFT_TM=59.780
PRIMER_RIGHT_TM=60.205
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=0.6004
PRIMER_LEFT_1_PENALTY=0.219884
PRIMER_RIGHT_1_PENALTY=0.380537
PRIMER_LEFT_1_SEQUENCE=ACATTCTTTGCCTTCTCACACA
PRIMER_RIGHT_1_SEQUENCE=GAACCGTCCTTGTCTGCATTAT
PRIMER_LEFT_1=13,22
PRIMER_RIGHT_1=168,22
PRIMER_LEFT_1_TM=59.780
PRIMER_RIGHT_1_TM=60.381
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.7727
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.552823
PRIMER_LEFT_2_SEQUENCE=ACATTCTTTGCCTTCTCACACA
PRIMER_RIGHT_2_SEQUENCE=ACAAACGCAGTCTTTGGTTTTC
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=289,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=60.553
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.9631
PRIMER_LEFT_3_PENALTY=0.757660
PRIMER_RIGHT_3_PENALTY=0.205455
PRIMER_LEFT_3_SEQUENCE=CATTCTTTGCCTTCTCACACAT
PRIMER_RIGHT_3_SEQUENCE=AAAGTTGCTTCACTTGAACCGT
PRIMER_LEFT_3=14,22
PRIMER_RIGHT_3=183,22
PRIMER_LEFT_3_TM=59.242
PRIMER_RIGHT_3_TM=60.205
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=170
PRIMER_PAIR_PENALTY_4=1.0969
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=0.876993
PRIMER_LEFT_4_SEQUENCE=ACATTCTTTGCCTTCTCACACA
PRIMER_RIGHT_4_SEQUENCE=ACGCAGTCTTTGGTTTTCCATA
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=285,22
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=60.877
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_04270 Ps_contig_mira-and-tgicl-ass_franssen_32688
SEQUENCE=ACATATCATCATTACATTCTTTGCCTTCTCACACATTCTCTCTCTCTCTCTCCAACTCTTTTCTCTTCTGTCACTTTGAACACAGCACAACAAAAATTACAAAACCTAATTCGGTTTAATTCGCTCTTATTTCTATTTACTCATATCATAATGCAGACAAGGACGGTTCAAGTGAAGCAACTTTCAGATCTAGCTACTGAGAGAGAGATTTACGAATTCTTCTCATTTTCTGGAGAAATTGAAAACATTCAGATCGTAAGAGAATATGGAAAACCAAAGACTGCGTTTGTGACATTTAAAGATCCAAAAGCACTTGAAATTGCGTTGTTGTTATCGGGAGCAACAATAGTAGACCAGATTGTGAGTATAAGTCCAGTTGAGAACTATGTTGCAAATCGTGAGATGCAAGAAGCAAGGGCAGGGGAGTATTCTATAACTATTGCTCCTTCTGAAGATGCCGCATTGAATTCCGAGGAGGAGAAACCCGGCGGAAGAGTATATCTTAGTAAAGCACAAGATGTAGTTACGAATATGCTGGCAAAAGGATCAGCTATAAAACAAGATGCCGTGAACAAAGCTAAAGCATTTGATGAGAAGCATCAATTGACTGCCAATGCATCAGCAAAGGTCAGTTCATTTGACAAGAGAGTTGGACTGACCGAGAAATTGACAGTTGGACTCTCTGTAGTGAATGAGAAAGTGAAATCTGTGGATCAGAGGCTACATGTTTCAGATAAAACAATGGCAGCAATATTTGCAGCCGAGAGGAAGTTGAACGATACTGGATCAGCTGTCAAAACAAGCAGATACGTGACTGCCGGAACATCATGGCTAACGGGTGCTTTTAGCAAGGTGGCGAAAGCAGGAAACGTGGCCGGTACTAAAACTAGAGAAAAGTTTAACATGGCTGTTTCAAACTTGACAACAAAGGATACCTCATTGGTTGTATAGGCCAGAATTCAGAATTTCGTTACGACGGTCACTTCATACGGTCTCAAGTGAAGATCTGTTTCAGAGCTGTTGGTGTGTTAAAAAGGTTTGGTCTTTGAGATCGATCATGTACTTGTTTCTTTCTCTCCTTCAACAGTTCAATGTTTGTAGTAGTTAGCAACTATAGTGATTCAATTCAATCCAATTTTTTGTATTCTTTTATCACTCTGGTGTTTTTTAGCTGTTGCTTCTATGTAATTGGTGATTTTGGCTTTTTTTTCTTCTTTACGTTTTGTCTATAGGAAGTTATAGCTGTTGCTTCTATGTAGAAGATAACATCTTGTTTTTGTTATCAACACTTGAAGGATTCATTTGGGAAATTTTTATCTTCTAGATTACTACCAACCGTGGAATTNGTATAGATATTTTTTTANTTNGTNATANTGATATTTAAG
TARGET=199,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7071
PRIMER_LEFT_PENALTY=0.154256
PRIMER_RIGHT_PENALTY=0.552823
PRIMER_LEFT_SEQUENCE=CACTTTGAACACAGCACAACAA
PRIMER_RIGHT_SEQUENCE=ACAAACGCAGTCTTTGGTTTTC
PRIMER_LEFT=71,22
PRIMER_RIGHT=289,22
PRIMER_LEFT_TM=59.846
PRIMER_RIGHT_TM=60.553
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.7080
PRIMER_LEFT_1_PENALTY=0.205455
PRIMER_RIGHT_1_PENALTY=0.502561
PRIMER_LEFT_1_SEQUENCE=ACGGTTCAAGTGAAGCAACTTT
PRIMER_RIGHT_1_SEQUENCE=GGTCTACTATTGTTGCTCCCGA
PRIMER_LEFT_1=162,22
PRIMER_RIGHT_1=354,22
PRIMER_LEFT_1_TM=60.205
PRIMER_RIGHT_1_TM=60.503
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.7583
PRIMER_LEFT_2_PENALTY=0.205455
PRIMER_RIGHT_2_PENALTY=0.552823
PRIMER_LEFT_2_SEQUENCE=ACGGTTCAAGTGAAGCAACTTT
PRIMER_RIGHT_2_SEQUENCE=ACAAACGCAGTCTTTGGTTTTC
PRIMER_LEFT_2=162,22
PRIMER_RIGHT_2=289,22
PRIMER_LEFT_2_TM=60.205
PRIMER_RIGHT_2_TM=60.553
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=128
PRIMER_PAIR_PENALTY_3=0.7727
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=0.552823
PRIMER_LEFT_3_SEQUENCE=ACATTCTTTGCCTTCTCACACA
PRIMER_RIGHT_3_SEQUENCE=ACAAACGCAGTCTTTGGTTTTC
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=289,22
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=60.553
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.8806
PRIMER_LEFT_4_PENALTY=0.205455
PRIMER_RIGHT_4_PENALTY=0.675181
PRIMER_LEFT_4_SEQUENCE=ACGGTTCAAGTGAAGCAACTTT
PRIMER_RIGHT_4_SEQUENCE=ATAGAATACTCCCCTGCCCTTG
PRIMER_LEFT_4=162,22
PRIMER_RIGHT_4=433,22
PRIMER_LEFT_4_TM=60.205
PRIMER_RIGHT_4_TM=60.675
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_04271 Ps_contig_mira-and-tgicl-ass_franssen_32690
SEQUENCE=CCGAACGACCAAGTAAGGGACCGAAAATTACGTTTGGTACCTACTTAATTTATACCCTCTCCGAATGAATACATCCATGACAATTTCGCAACACAAAAACTTCTTTCACCCTCTCCTGCTCTGTCTATAGAACATCTCTTCCCGAAGTTTCTTGATCAAGTTTATAGCGGAGCCTTCCTAATCGCCCCTCGGAATCATCACGAACAGCGACACATTCAGGTGGAGTTTCAAACAGAGACAAAGAGAATTGTTTATAAGCAGCACCAACATCAAAGTAAATCAAACCAGAGCTAGGAACATCAACACGAATACTCTTAACAGTCAACCAAAGAAAAAGATCCTGCGCTTCTAACCCTGTCAACCCATCAATCTTCGCATAACTCAATGACCCAGAAACGTTTCGATCATAATGCAACTCGTTCTCGAATTTGGCGTTACAAGCTTGATCTAAATGTACCCAGAATTTACCATCTTCACCAACGTTGAATTGGGTAACACCCTTGGGGAAAATACCCATGGGAAGACCGTGGTTGCGGAGAACCTCGTAGATTGAAGTTGCATTTTTTGATACAGAAAATGCATAAGAAGAAGAAATGGTTGAGATTGAGAGAACGAGGAGAAGAAGAAATTGAAACATGTTTGTGAGCTTTAAGGTTATGATGTTAGAGCATTGGATTGAAGAGTGAAATGGGTTTTGGGATTGGTGAAGAGAAGAAGGAGAGAGAGAGAGAGAGAGAGGTAGACAGTCGTTGARTNAACCCGTACCCCCGGTACCGTTACTTCGTTCGG
TARGET=718,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04272 Ps_contig_mira-and-tgicl-ass_franssen_32694
SEQUENCE=AAAGTTNTTTTTAACTAGAACAAATTTTATTATATAATATACCCTGTATATCAATTTCACATCAAGTAAATATTCATATTCAAATACATTCCAATGATAATATTGAATAAATGAACGAGAATTGATGAAAGGTTATACATATAGAAGGTGTAGTAGTATTGTATATAAGTTTGGTGATTAATTTCACTTGGCCATCATAACCTTAACAAATTCATCATAATTAATTTGACCATCGCCATCAACATCAGCCTCTCGAATCATCTCATCGACTTCTTCATCAGTTAGCTTCTCGCCAAGATTGGTCATTACATGACGAAGTTCGGCAGCAGAAATGAATCCGTTCTGATCCTTATCGAATACTCTGAATGCTTCTTTCAGCTCCTCCTCTGAATCTGTGTCTTTCATTTTCCTTGCCATAAGGTTTAGAAATTCAGGGAAATCGATTGTTCCGTTACCATCAGCATCGACCTCGTTTATCATGTCTTGTAGCTCTGCCTCTGTTGGATTTTGGCCTAATGACCTCATCACAGTTCCAAGCTCCTTTGTTGTGATGGAACCATCGCCATCTTTATCAAACAAGCTGAAGGCTTCCTTGAATTCAGAGATCTGATCATCTGTGAGTTGATCTGCCATTGTGAAATATAGATTCTTTTCTTTTCTTTGGATTATTTGTTTTAGTTTTTTTTTTTCTTATTGTTCTTTTACGAAAGGAAAAGGAAAGGAAGGAAGGAAAGGAAAAAGAAAAGAAAAAACGAAAGGTAAAGTAAGAAAACTAAACTAACTTCTTTACGTTAACCCTAAACCTAAAACCCTTCCTT
TARGET=647,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1365
PRIMER_LEFT_PENALTY=0.086698
PRIMER_RIGHT_PENALTY=0.049761
PRIMER_LEFT_SEQUENCE=GTAGCTCTGCCTCTGTTGGATT
PRIMER_RIGHT_SEQUENCE=TCCTTCCTTTCCTTTTCCTTTC
PRIMER_LEFT=485,22
PRIMER_RIGHT=726,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=60.050
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=0.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.1365
PRIMER_LEFT_1_PENALTY=0.086698
PRIMER_RIGHT_1_PENALTY=0.049761
PRIMER_LEFT_1_SEQUENCE=GTAGCTCTGCCTCTGTTGGATT
PRIMER_RIGHT_1_SEQUENCE=TTCCTTCCTTCCTTTCCTTTTC
PRIMER_LEFT_1=485,22
PRIMER_RIGHT_1=731,22
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=60.050
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=0.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.1365
PRIMER_LEFT_2_PENALTY=0.086698
PRIMER_RIGHT_2_PENALTY=0.049761
PRIMER_LEFT_2_SEQUENCE=GTAGCTCTGCCTCTGTTGGATT
PRIMER_RIGHT_2_SEQUENCE=TTTCCTTTCCTTCCTTCCTTTC
PRIMER_LEFT_2=485,22
PRIMER_RIGHT_2=737,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=60.050
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.1365
PRIMER_LEFT_3_PENALTY=0.086698
PRIMER_RIGHT_3_PENALTY=0.049761
PRIMER_LEFT_3_SEQUENCE=GTAGCTCTGCCTCTGTTGGATT
PRIMER_RIGHT_3_SEQUENCE=TCTTTTTCCTTTCCTTCCTTCC
PRIMER_LEFT_3=485,22
PRIMER_RIGHT_3=741,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=60.050
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.1676
PRIMER_LEFT_4_PENALTY=0.117817
PRIMER_RIGHT_4_PENALTY=0.049761
PRIMER_LEFT_4_SEQUENCE=GAGTTGATCTGCCATTGTGAAA
PRIMER_RIGHT_4_SEQUENCE=TCTTTTTCCTTTCCTTCCTTCC
PRIMER_LEFT_4=618,22
PRIMER_RIGHT_4=741,22
PRIMER_LEFT_4_TM=60.118
PRIMER_RIGHT_4_TM=60.050
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=0.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_04273 Ps_contig_mira-and-tgicl-ass_franssen_32694
SEQUENCE=AAAGTTNTTTTTAACTAGAACAAATTTTATTATATAATATACCCTGTATATCAATTTCACATCAAGTAAATATTCATATTCAAATACATTCCAATGATAATATTGAATAAATGAACGAGAATTGATGAAAGGTTATACATATAGAAGGTGTAGTAGTATTGTATATAAGTTTGGTGATTAATTTCACTTGGCCATCATAACCTTAACAAATTCATCATAATTAATTTGACCATCGCCATCAACATCAGCCTCTCGAATCATCTCATCGACTTCTTCATCAGTTAGCTTCTCGCCAAGATTGGTCATTACATGACGAAGTTCGGCAGCAGAAATGAATCCGTTCTGATCCTTATCGAATACTCTGAATGCTTCTTTCAGCTCCTCCTCTGAATCTGTGTCTTTCATTTTCCTTGCCATAAGGTTTAGAAATTCAGGGAAATCGATTGTTCCGTTACCATCAGCATCGACCTCGTTTATCATGTCTTGTAGCTCTGCCTCTGTTGGATTTTGGCCTAATGACCTCATCACAGTTCCAAGCTCCTTTGTTGTGATGGAACCATCGCCATCTTTATCAAACAAGCTGAAGGCTTCCTTGAATTCAGAGATCTGATCATCTGTGAGTTGATCTGCCATTGTGAAATATAGATTCTTTTCTTTTCTTTGGATTATTTGTTTTAGTTTTTTTTTTTCTTATTGTTCTTTTACGAAAGGAAAAGGAAAGGAAGGAAGGAAAGGAAAAAGAAAAGAAAAAACGAAAGGTAAAGTAAGAAAACTAAACTAACTTCTTTACGTTAACCCTAAACCTAAAACCCTTCCTT
TARGET=719,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1191
PRIMER_LEFT_PENALTY=0.117817
PRIMER_RIGHT_PENALTY=1.001257
PRIMER_LEFT_SEQUENCE=GAGTTGATCTGCCATTGTGAAA
PRIMER_RIGHT_SEQUENCE=GGAAGGGTTTTAGGTTTAGGGTT
PRIMER_LEFT=618,22
PRIMER_RIGHT=815,23
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.999
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=1.1424
PRIMER_LEFT_1_PENALTY=0.117817
PRIMER_RIGHT_1_PENALTY=1.024629
PRIMER_LEFT_1_SEQUENCE=GAGTTGATCTGCCATTGTGAAA
PRIMER_RIGHT_1_SEQUENCE=AGGAAGGGTTTTAGGTTTAGGG
PRIMER_LEFT_1=618,22
PRIMER_RIGHT_1=816,22
PRIMER_LEFT_1_TM=60.118
PRIMER_RIGHT_1_TM=58.975
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=199
PRIMER_PAIR_PENALTY_2=1.1428
PRIMER_LEFT_2_PENALTY=0.141511
PRIMER_RIGHT_2_PENALTY=1.001257
PRIMER_LEFT_2_SEQUENCE=CATCTGTGAGTTGATCTGCCAT
PRIMER_RIGHT_2_SEQUENCE=GGAAGGGTTTTAGGTTTAGGGTT
PRIMER_LEFT_2=611,22
PRIMER_RIGHT_2=815,23
PRIMER_LEFT_2_TM=60.142
PRIMER_RIGHT_2_TM=59.999
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=1.1661
PRIMER_LEFT_3_PENALTY=0.141511
PRIMER_RIGHT_3_PENALTY=1.024629
PRIMER_LEFT_3_SEQUENCE=CATCTGTGAGTTGATCTGCCAT
PRIMER_RIGHT_3_SEQUENCE=AGGAAGGGTTTTAGGTTTAGGG
PRIMER_LEFT_3=611,22
PRIMER_RIGHT_3=816,22
PRIMER_LEFT_3_TM=60.142
PRIMER_RIGHT_3_TM=58.975
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=1.1800
PRIMER_LEFT_4_PENALTY=0.178718
PRIMER_RIGHT_4_PENALTY=1.001257
PRIMER_LEFT_4_SEQUENCE=CTGTGAGTTGATCTGCCATTGT
PRIMER_RIGHT_4_SEQUENCE=GGAAGGGTTTTAGGTTTAGGGTT
PRIMER_LEFT_4=614,22
PRIMER_RIGHT_4=815,23
PRIMER_LEFT_4_TM=60.179
PRIMER_RIGHT_4_TM=59.999
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_04274 Ps_contig_mira-and-tgicl-ass_franssen_32702
SEQUENCE=CTTTCANGTTTTTCCTTTGTCCATGACACCCTATTCCTCCTAAAGCAAAACTTGAGAACAATACAACAGCTTTTTTATTACTCGTATAAATGCGTGTAACATCCGATTTTCAAGAGAAGTTTCTTTCCATGTCGTAGTTTGGTTTTAGGATTAGATTACAAAGGAAAAGAGCTTCTGTCTTAAGCGAATCATCTGAACGACGATGGACAGGCAATAGAGGTTACATTTTATACAAAATAAGAAAGGGATTACTGACAAGGTGGATCTGGATTGAACTTCACAGATTCCTTCCATATGAGCTCCTTGATATGATCTTCAGTGCATGTTGGCTGATCAAAATCAAAAGTGAATGGCCTTGGACACACCGGCTCCTCGTTCGTGTTGTGAAGCGATGAAAGATACGGGTGACACAGTGCCTCATCAACTGTAATGCGTTTGTTTGGATCAAAGATAAGCATCTTTTCTAACAGGTCAAGTGCCTCGGGTAACATGTTTGGGAATCTAGACGAGAATTTTTGCTTCCGGTATTGTTGAAACTGTCTAAAATATCTTCTAGCATTGTCACTTCGGAGAAATCCAAGGCTAGCATCATCGGGCGAACCTATTAACTCTGTGATAAGCCTTAGTTGATGAACATAGTCTTTGCCAGGAAACAAGGGCTCTCTGGTCATGATTTCACCAAATATGCAACCGACAGACCAAACATCAATCGCAGATGTGTACTCAGAACAATTGAGGAGTAACTCCGGAGCTCGGTACCATCTCGTGACAACATACTCAGTCATGAAATCAGTTTCAGATGTTGTCCTTGCCAAACCGAAGTCGCCGATTTTAAGGTCACAGTTTGTGTTCACTAGTAAATTACTCGGCTTAAGATCACGGTGCAAAACATTAACTGAGTGCACATATTTCAGCCCACGTAACAGCTGATATAAGAAGTACTGACAATGTTCTTCGCAGAGAGGTTGATCAGAATGAATTATATGATGGAGATTAGTGTCCATCAAATCATAAACAATGTACACATCATTAAACGCGTCCTTTTTCGGGGGTCGTATGATATCCTTGATGGCAATGATATTTTGATGATCCATGTGACGGAGAAGCTTGATTTCGCGCAAAGTCCTTTTCGCATCAACTATATTGTCAAAAGCATTACCAATCTTCTTAATAGCAACTTGTTCATGTGTATCGGAATTAACAGCAGCACTATAAAGAAACATTGTTCAAGTGACAGTAAAATCATTTTCCAACAATCAAAAACAAAACACCGAATTCATTCATGCATTTACCTCGTCCTATGAAAGAAAGAAACAAAAAACGCTTACCAAACGATACCATACGCGCCTCTACCAATAGGACGAATGGGAGGCACGTACTTAGAAGACACCTCAAACAAGTTTCCATACACATTGTAGTGTGCATATTTACCACCATGAGTAAGAACCCTTTTGATTTTAGTGTCCGCAGAAGCCGAAGCAGAAGCAGAAGAGCTTGACTCTTTTGTTGCCATGTTGAAACTTGAACAAAGGGTCTTCACCTTTGTTCTTGGAACAATGTAATGGAAGCTAATGTTGACACATAGAACAA
TARGET=1303,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0456
PRIMER_LEFT_PENALTY=0.033470
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TCATTCATGCATTTACCTCGTC
PRIMER_RIGHT_SEQUENCE=CAAAGGTGAAGACCCTTTGTTC
PRIMER_LEFT=1276,22
PRIMER_RIGHT=1544,22
PRIMER_LEFT_TM=59.967
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=269
PRIMER_PAIR_PENALTY_1=0.0456
PRIMER_LEFT_1_PENALTY=0.033470
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=TCATTCATGCATTTACCTCGTC
PRIMER_RIGHT_1_SEQUENCE=AAAGGTGAAGACCCTTTGTTCA
PRIMER_LEFT_1=1276,22
PRIMER_RIGHT_1=1543,22
PRIMER_LEFT_1_TM=59.967
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.0547
PRIMER_LEFT_2_PENALTY=0.033470
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=TCATTCATGCATTTACCTCGTC
PRIMER_RIGHT_2_SEQUENCE=TTCTAAGTACGTGCCTCCCATT
PRIMER_LEFT_2=1276,22
PRIMER_RIGHT_2=1384,22
PRIMER_LEFT_2_TM=59.967
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=0.1497
PRIMER_LEFT_3_PENALTY=0.137550
PRIMER_RIGHT_3_PENALTY=0.012153
PRIMER_LEFT_3_SEQUENCE=ATTCATGCATTTACCTCGTCCT
PRIMER_RIGHT_3_SEQUENCE=CAAAGGTGAAGACCCTTTGTTC
PRIMER_LEFT_3=1278,22
PRIMER_RIGHT_3=1544,22
PRIMER_LEFT_3_TM=59.862
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.1497
PRIMER_LEFT_4_PENALTY=0.137550
PRIMER_RIGHT_4_PENALTY=0.012153
PRIMER_LEFT_4_SEQUENCE=ATTCATGCATTTACCTCGTCCT
PRIMER_RIGHT_4_SEQUENCE=AAAGGTGAAGACCCTTTGTTCA
PRIMER_LEFT_4=1278,22
PRIMER_RIGHT_4=1543,22
PRIMER_LEFT_4_TM=59.862
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_04275 Ps_contig_mira-and-tgicl-ass_franssen_32704
SEQUENCE=CGTTTTCTAANTTTATCAAAATCCAGTTCAAATTAACAGAATCTAACAAAACTCTGCCAGTTCAAAGTCGTATGGTATTACTAACTCAGATCCCTGTTAGTATTTTCTTGGACAAAGCTCAACTTAGTGCGAAGAAAAACAATAGCCATAAAAATGCTCATAAGCTACATACAGGTTACAACACAGACTGGTCTTTTCAACCTAAATTTTAGAACTTCTTTCTCTCTCTCCAACCTAGTCTATTACATGATTCTGTACTCCGTGGAAGGGTCTCAGTCTCGCAGGACTGAGCTAGAGAAGAGTTATTAATCTACTTCTTCTTAGATTTGCTTGACGTAGACCGAGTATACGCAGTTCCCTCAAGCCATTCATCCATTGAAGGATCTGTAGTCTTAGTCCCAACTTCAACTTTGGAAGATCTTTTTGGTTTCTTAAGGTTCTGTATTTGACCATTAATCCATAGCCCAAGTAGGACAAGAAGTGCAGTTCCAAGAGTAAAAAGAAAAGCAGATTCCATGCTGAATAAAACACCAGGCTCAACAACTTCAATTGTTCCATTATAGAAGGTGTTTTGGTATGGATGTTGGTCTATCTCATAGACAATGGTGCCCACAAGATCAAAAGGTCCAGGCTGCAAGAACTTGCTGACAGAAAATATATATGGGAAACTAGCCTGAGCTGATGATGGCACAGAGCCATTGTTGAAAACCTGAACAGTAAGGTTTTGAACAAGAAGATGACGATCAAATGGAAGATGAACACTAGCCTTAATAGCAACGACATTCAAACTTGACTGCCCATCATTTTTCAGACCAACAAGCAGCTCTGCCTCTTCACCACCTTTTACCAATCTTGCAATATTCTTAGGGAAGACACAGATGGTATCAACTCCCGGAGCAGAAGGAAAGCCTCCATCCGCAAAGTCTTGAGCGTCATCAGTTTCATCACCAACAATTCCAATGTCACTCACTTCACCAGCATTCGCTGCAGCTTCATCTGACTGACACCTAACAACTTGGAGGAGTGGGGAAGCGATGAGGAAGAGAAGGAAGGAGAGAATCCAGAAGTTGTTGATGAGCGCCATGTGGATGGTTCTAGGGTTGTGTTGGAAATGGAAATGGAAGTGGAAAAGGAATATAGTAGAAACGAGAAAAACAGAGAAAGAAGATGGAATGTGTGAGTGATAACCGTTGTCGTTTCATTCCCGTGTACAGAACAATGCCGTCGTTTTGATCAATGAAATTATCGCTACTTTTACTTACTTGACTTCACTGT
TARGET=221,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2602
PRIMER_LEFT_PENALTY=0.201304
PRIMER_RIGHT_PENALTY=0.058853
PRIMER_LEFT_SEQUENCE=AGTGCGAAGAAAAACAATAGCC
PRIMER_RIGHT_SEQUENCE=TACTCGGTCTACGTCAAGCAAA
PRIMER_LEFT=125,22
PRIMER_RIGHT=346,22
PRIMER_LEFT_TM=59.799
PRIMER_RIGHT_TM=59.941
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.3600
PRIMER_LEFT_1_PENALTY=0.301100
PRIMER_RIGHT_1_PENALTY=0.058853
PRIMER_LEFT_1_SEQUENCE=GACAAAGCTCAACTTAGTGCGA
PRIMER_RIGHT_1_SEQUENCE=TACTCGGTCTACGTCAAGCAAA
PRIMER_LEFT_1=110,22
PRIMER_RIGHT_1=346,22
PRIMER_LEFT_1_TM=59.699
PRIMER_RIGHT_1_TM=59.941
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=0.3637
PRIMER_LEFT_2_PENALTY=0.304841
PRIMER_RIGHT_2_PENALTY=0.058853
PRIMER_LEFT_2_SEQUENCE=TCTGCCAGTTCAAAGTCGTATG
PRIMER_RIGHT_2_SEQUENCE=TACTCGGTCTACGTCAAGCAAA
PRIMER_LEFT_2=52,22
PRIMER_RIGHT_2=346,22
PRIMER_LEFT_2_TM=60.305
PRIMER_RIGHT_2_TM=59.941
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.3936
PRIMER_LEFT_3_PENALTY=0.201304
PRIMER_RIGHT_3_PENALTY=0.192310
PRIMER_LEFT_3_SEQUENCE=AGTGCGAAGAAAAACAATAGCC
PRIMER_RIGHT_3_SEQUENCE=ACTCGGTCTACGTCAAGCAAAT
PRIMER_LEFT_3=125,22
PRIMER_RIGHT_3=345,22
PRIMER_LEFT_3_TM=59.799
PRIMER_RIGHT_3_TM=60.192
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.4022
PRIMER_LEFT_4_PENALTY=0.343387
PRIMER_RIGHT_4_PENALTY=0.058853
PRIMER_LEFT_4_SEQUENCE=AACTCTGCCAGTTCAAAGTCGT
PRIMER_RIGHT_4_SEQUENCE=TACTCGGTCTACGTCAAGCAAA
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=60.343
PRIMER_RIGHT_4_TM=59.941
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_04276 Ps_contig_mira-and-tgicl-ass_franssen_32707
SEQUENCE=CCAAAATTAGTTTTTGTTTTAAGAAGCTAAATTAAGCTGAGAGGAAAAGCAATATAGTGAGAGAAGCTGATCGAGTTTGTTGCGTCGCGAAGAGTGAAAAATGTCACACAGCGACACCATCCCCCTTCACGCATCTTCCCAATCTGACATCGACGAGATCGAGAATCTCATCTATGCTTCTCCGGCCACCGTTCTTCCGGCGAGACCTCCAAGTCCACCACGCGCTTCGATTCCCGTTTCATCCTCTTCTCCTTTCATCAATTCCAATCTCCCTAATCCCGCTCCTCCCAAATCCTCTTACCAACCTCAAATTCCAAAACCTCCTTCTTCTTCTTCCTCTTTCCCTCCTCCTCCACCTCCTCCTTCTACTCGTCCTGATATCTCTAATTCTGGATTCGGACCTGCTCCGAACACGCTCACCGAACCGGTTTGGGATACGATCAAGAGAGATCTGTCGCGGATCGTTAGTAATTTGAAGCTTGTTGTGTTTCCTAACCCTAATCGGGAAGATCCGGGTAAGGCGTTGCGTGATTGGGATCTATGGGGACCTTTCTTTTTCATTGTGTTCCTTGGTCTCATTCTTTCTTGGTCTGCGTCTGTCAAAAAGTCTGAGGTGTTTGCAGTTGCGTTTGCCTTACTTGCTGCTGGTGCTGTAATTTTGACATTGAATGTTCTGCTTCTGGGTGGACACATTATTTTCTTCCAAAGTCTGAGTCTGCTGGGTTATTGCTTATTCCCTCTTGATGTTGGCGCATTAATTTGCATGTTGAAGAACAATGTTATATTGAAAATGGTAGTGGTATGTGTGACTTTAGCGTGGAGCTCTTGGGCTGCATATCCTTTCATGAGTGCTGCAGTGAATCCCAGGAGAAAAGCTCTTGCTCTATACCCAGTTTTTCTCATGTATGTATCTGTTGGTTTTCTCATCATTGCTATTGACTAAATGCATCAATGTTTCAGATAATTACTTGGAATTCCCTTCTAAATAGGAATTTTTGCTTAATGATCAACCCTTTTTATTCTTTGTTGCCCTTCTTTTGTACTTGATGTCTTCTTCTTTTCTTTTGATATTTTTGCTAGATATAGTATTTGGAGACATACGTTGTATAATATTACATGGAATATATGATGGAATCAAAGTTGCTGCAAGNCATCTCGTTC
TARGET=324,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2321
PRIMER_LEFT_PENALTY=0.222735
PRIMER_RIGHT_PENALTY=0.009409
PRIMER_LEFT_SEQUENCE=CTTTCATCAATTCCAATCTCCC
PRIMER_RIGHT_SEQUENCE=TCAGGACGAGTAGAAGGAGGAG
PRIMER_LEFT=251,22
PRIMER_RIGHT=377,22
PRIMER_LEFT_TM=59.777
PRIMER_RIGHT_TM=60.009
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=127
PRIMER_PAIR_PENALTY_1=0.2520
PRIMER_LEFT_1_PENALTY=0.149516
PRIMER_RIGHT_1_PENALTY=0.102490
PRIMER_LEFT_1_SEQUENCE=CAACCTCAAATTCCAAAACCTC
PRIMER_RIGHT_1_SEQUENCE=CCAAGAAAGAATGAGACCAAGG
PRIMER_LEFT_1=301,22
PRIMER_RIGHT_1=588,22
PRIMER_LEFT_1_TM=59.850
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.2990
PRIMER_LEFT_2_PENALTY=0.149516
PRIMER_RIGHT_2_PENALTY=0.149516
PRIMER_LEFT_2_SEQUENCE=CAACCTCAAATTCCAAAACCTC
PRIMER_RIGHT_2_SEQUENCE=CACAATGAAAAAGAAAGGTCCC
PRIMER_LEFT_2=301,22
PRIMER_RIGHT_2=564,22
PRIMER_LEFT_2_TM=59.850
PRIMER_RIGHT_2_TM=59.850
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=264
PRIMER_PAIR_PENALTY_3=0.3100
PRIMER_LEFT_3_PENALTY=0.149516
PRIMER_RIGHT_3_PENALTY=0.160449
PRIMER_LEFT_3_SEQUENCE=CAACCTCAAATTCCAAAACCTC
PRIMER_RIGHT_3_SEQUENCE=GGATCTTCCCGATTAGGGTTAG
PRIMER_LEFT_3=301,22
PRIMER_RIGHT_3=512,22
PRIMER_LEFT_3_TM=59.850
PRIMER_RIGHT_3_TM=60.160
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.3790
PRIMER_LEFT_4_PENALTY=0.149516
PRIMER_RIGHT_4_PENALTY=0.229480
PRIMER_LEFT_4_SEQUENCE=CAACCTCAAATTCCAAAACCTC
PRIMER_RIGHT_4_SEQUENCE=CCGATTAGGGTTAGGAAACACA
PRIMER_LEFT_4=301,22
PRIMER_RIGHT_4=504,22
PRIMER_LEFT_4_TM=59.850
PRIMER_RIGHT_4_TM=60.229
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=204
=
PRIMER_SEQUENCE_ID=SSR_04277 Ps_contig_mira-and-tgicl-ass_franssen_32709
SEQUENCE=CTACCCCTCGTTGCGGTGGTCGTCCTAGGTGGAAAAGGTAAGAACAATTTACGTATAGGAGTTACATCTTACAGAAGGAAGTACAGAANGCACTTGGTGGAAAATACAGTAGTGACATGCCACAGGTTTTTATCAGAGGGAAACATGTTGGAAATGCGGAGGATTTGAAACAATTGAATGAATCCGGTGAATTGGCGAAACTGTGAGTGGTTTTCCAACTCAAGATCTTTGGTTTGTTTGTGATAAATGTGGTGATGCAAGGTTTGTGCCTTGTGATAATTGCAATGGTAGCAGGAAAGTGTTTGAGGAAGAACAAGGGAAGTTGAAAAGGTGTGTTCATTGTAATGAGAATGGATTGATAAGATGCACAAGCTGTTGTTCATGAATCAGTGATTACCAAAATTACTGATTGATTGATGTTATTCTTTTTTATTGTCTTGTTTTTTGTTTTTTCTTTGGGTTTGTAGTTTTGTTTATTCTGTAA
TARGET=407,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04278 Ps_contig_mira-and-tgicl-ass_franssen_32711
SEQUENCE=TGGTATCAACGYNNNGTTCGGGGAGGACACTCAAAACATTCTAATCTCTTTCAATTCTCTTCACTTTTGAAATTTTTTGAATCTCTTTTTTTCATCTTTCTACTATGGCTTCCTACACCGTCGTTTTGATGCTAGTTGCCACTTTGTTGGTGAGTTCCACCATTGCTCAATCTCCAGCATCATCTCCAACCAGATCGCCGGTTGCCTCTCCACCAAAGTCTTCTCAGTCTCCGGCTCCAGCGGTTTCTCCCTCCGCTAACTCCCCGGCGGCATCTCCTCCCGTACCTGTGAAGAACGCTCCATCTCCTTCACCATCCACCATCAGCTCTCCACCATCTCCTTCTTCCCCTTCCCCTGCTACTCCATCCACCGCTCCTGCCGTTACTCCATCAACCATCACTAATCCTCCTTCTGAAGCACAATCACCTTCCGATAGCGGCGCTGGTTTGAACAGATTCACCGTCGCCGGATCTGCTGCTGTTGTGGTTTTCGCTGCTGCTTTGATGATGTAGAGAACTACTTTTTCGTTTGATCTATTTTGTTTTATATCACGAATATATATGAATCGTGAACTTTTTACCTTTTTTTTGCTTTGATTTGATCTGTGTGATGACTTATTCATTTGATTAATTTTGTAATCTATTTATTTATTTAATGAGTGATACGTTTTATTCCTTTTANNNNNNCTTGTTTCCTTTAGATTATTTTATGGTACTA
TARGET=641,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04279 Ps_contig_mira-and-tgicl-ass_franssen_32739
SEQUENCE=CTAAACCCTACATTTTCTTCATTCTTCAATAAGTGAGAGATAATAGATATTATCTATGGAGGGTGGTGAGAGAATCGGAGAAGATATGGATCTCGTTCATTCTGAAAATGCTGCTGCTGCTGCCGCTGTTGGTGTTGGTTCCGTTTCTCAGGATCAAGAAATGGTTGATCAAGCTGATGCTGATACAACAATTTCAAATGTCCATTCTACCCCTACTCCTCAGGCTAAAAGTTAAGAATGTGACAGAAATGAATAATCATAGTGTGATAAACAGTGCTGGCGAGATGAGTGGTTATGATGAGACTGGTACTGCTATGGAACGAGAAGCGTTTATGAAAGAGCTTGAGAGTTTTTATAGATCGAGGTCGTTAGAATTTAAGCCTCCTAAGTTTTATGGAGTGCCGTTGAATTGTCTCAAGTTATGGAGAGCTGTTATCAAACTGGGTGGCTATGAATTGGTAACTGGATCGAAGTTGTGGCGGCAAGTAGGAGAGTCTTTCCACCCTCCTAAGACTTGCACTACAGTCTCATGGACGTTTAGAATCTTTTATGAGAAGGCGCTTTTAGAGTATGAAAAGCATAAGAGAGAGACCGGGGAGCTACAGCTCCCTGTTGGATCACTCCATCAACCTTCAAGTGTGGAAAAAGAGACCACTGTTTATCAGGCTCCAGGCTCAGGTAGAGCACGAAGGGATGCAGCAGCACGTGCAATGCAAGGTTGGCATGCTCAGCGCCTTCTTGGATATGGTGAGGTTGCTGAGTCAGCTGTTAAGGACAAGAACTTCAGTCCTACACAAAAACGTGAAAAGAACCTCAAAAGTATTGGTGTGATCAACAAACAGAGGATGCCATCTGGTATGGACCATGCTGACAAAACTGCAAATATAGAAGGAGACAGGCAATTGGTCACTGCAGTAGTGGATATTGGACCCCCAGCTGACTGGGTTAAGATCAATGTGCGGGAAACGAAGGATTGTTTTGAAGTGTATGCGCTGGTTCCTGGGCTCCTTCGCGAGGAGGTGCGAGTTCAATCCGATCCAGTTGGACGACTTGTTATAACTGGTACGCCAGAACATGTTGACAATCCATGGGGTATCACCCCCTTCAAAAAGGTTGTGAACTTACCTTCGCGAATTGATCCACTTCAGACATCTGCCATTGTTAGTTTGCACGGCCGACTATTTGTACGCGTTCCTTTTGAGCAGGGAGCTGTATGATTTTTATTTCCCCCCCTTGAATGTTTGGTTTCTCACAATTCCTCAAAATGAGTAAAGTTTTTATAAACAATCAAGGCATGGTTATGGTAGGAACTAGTCTAGAGTTCTGTTGGATTAGTTCAAACTAATGTCCAACTTTACTATCAATAATTTATTTTCGCTACGGTAAAATACCCAA
TARGET=109,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0550
PRIMER_LEFT_PENALTY=0.042918
PRIMER_RIGHT_PENALTY=0.012045
PRIMER_LEFT_SEQUENCE=ATCTATGGAGGGTGGTGAGAGA
PRIMER_RIGHT_SEQUENCE=GCCTGAGGAGTAGGGGTAGAAT
PRIMER_LEFT=51,22
PRIMER_RIGHT=224,22
PRIMER_LEFT_TM=59.957
PRIMER_RIGHT_TM=59.988
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=0.0878
PRIMER_LEFT_1_PENALTY=0.042918
PRIMER_RIGHT_1_PENALTY=0.044904
PRIMER_LEFT_1_SEQUENCE=ATCTATGGAGGGTGGTGAGAGA
PRIMER_RIGHT_1_SEQUENCE=TGAGGAGTAGGGGTAGAATGGA
PRIMER_LEFT_1=51,22
PRIMER_RIGHT_1=221,22
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=171
PRIMER_PAIR_PENALTY_2=0.2239
PRIMER_LEFT_2_PENALTY=0.211806
PRIMER_RIGHT_2_PENALTY=0.012045
PRIMER_LEFT_2_SEQUENCE=TGGTGAGAGAATCGGAGAAGAT
PRIMER_RIGHT_2_SEQUENCE=GCCTGAGGAGTAGGGGTAGAAT
PRIMER_LEFT_2=63,22
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=60.212
PRIMER_RIGHT_2_TM=59.988
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=0.2567
PRIMER_LEFT_3_PENALTY=0.211806
PRIMER_RIGHT_3_PENALTY=0.044904
PRIMER_LEFT_3_SEQUENCE=TGGTGAGAGAATCGGAGAAGAT
PRIMER_RIGHT_3_SEQUENCE=TGAGGAGTAGGGGTAGAATGGA
PRIMER_LEFT_3=63,22
PRIMER_RIGHT_3=221,22
PRIMER_LEFT_3_TM=60.212
PRIMER_RIGHT_3_TM=59.955
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=0.2742
PRIMER_LEFT_4_PENALTY=0.042918
PRIMER_RIGHT_4_PENALTY=0.231323
PRIMER_LEFT_4_SEQUENCE=ATCTATGGAGGGTGGTGAGAGA
PRIMER_RIGHT_4_SEQUENCE=TTCTCGTTCCATAGCAGTACCA
PRIMER_LEFT_4=51,22
PRIMER_RIGHT_4=325,22
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=59.769
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_04280 Ps_contig_mira-and-tgicl-ass_franssen_32750
SEQUENCE=TCAACGCAGAGTATCGGGGATAACAATCAACCCCATTCCTTCTTTAACACAGCTCCCTACTTCTTAGCGAGTAAGAAACAAAACCAATCTCTCTCTCTCTAGAAAACTCCTCTTTTTTCTCCTTGTCTTTACACCAAAAAAAATGCATAACCCCCATAAGCCCACCGCCCACGAATCTGATCCTCAGCTACCAGTAATCAGAATCCACCACCCGTCCTCCCCTCGCCACCAGTCCGCCGCCACACCCACTCCCACTGCCGGCGCCCGCCGAAAAATCGGCGTAGCCGTTGACCTCTCCGACGAGAGCGCGTACGCTGTACGCTGGGCAGTACAACACTACATCCGTCCCGGTGACGCCGTCATCCTCCTCCACGTCAGCCCTACCAACGTCCTCTTCGGTGCTGACTGGGGTTCCATCGATCTCACCATTAACACAGACCCTAATTACGAAGAAGAAAACATCAACGGCGTCAACAACAACAATCTTAATGAAACCCCGACGCCTAAGCGGAAGCTCGAAGAAGATTACGACGCGTTCACGGCAACGAAATCAGCCGATCTTGCGAAACCGCTTCGTGAAGCTCAAATTCCGTACAAGATCCACATCGTGAAGGACCATGACATGAAGGAGCGTCTTTGTCTTGAGGTTGAGCGTCTTGGTCTTAGTGCTGTTATTATGGGGAGCCGCGGATTCGGTGCGGCGCGTCGCGGTAGTGATGGGAAGCTGGGAAGTGTTAGTGATTACTGTGTTCATCATTGTGTCTGCCCTGTTGTGGTTGTTCGCTATCCGGATGATAAGGATGCTGCTGAGGCTGTTGTTGCTGTCAAAGAGGGTGATGAGGGTGAAGCTGTTATCAAGCCTCTTTCTGTTGCGCATGAACATAAGAAAGAAGATTAGTGGGAGCCAATTCATCACTTCAACCCTGTCTCCGCAGGTTGTACTATTGAGCATGTTCCTGGTTGTTAACTCTGGAAAGATTATGGATGGTTTTTTTATGGCTTGATGATACTAGTTCCTTTAAGCTGTTGGAAGGTTATGTTTGGTTCAGAAGTGGCATGTACTGTTTCATGTAGATGTACAAGTTTGCTGTGTGACCCTTTGGGGTTATTTCCCATTCTGTATGTGGGGGAAATCGAATTATCTTGATGAGCACTTATTTAGCTAGTAGATAAATTCATAGAGCTATGTTGTTGTTGATGGGACTATGTCTGTTTTGAGTTCAGCACATTTACCCGGTGTTAAAAAAGAAAAGAAAAATTAAACTGAAATCTTGTTGATGATAG
TARGET=88,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2600
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.259289
PRIMER_LEFT_SEQUENCE=CCCATTCCTTCTTTAACACAGC
PRIMER_RIGHT_SEQUENCE=GGTAGCTGAGGATCAGATTCGT
PRIMER_LEFT=31,22
PRIMER_RIGHT=191,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.741
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=0.3138
PRIMER_LEFT_1_PENALTY=0.054514
PRIMER_RIGHT_1_PENALTY=0.259289
PRIMER_LEFT_1_SEQUENCE=TAACAATCAACCCCATTCCTTC
PRIMER_RIGHT_1_SEQUENCE=GGTAGCTGAGGATCAGATTCGT
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=191,22
PRIMER_LEFT_1_TM=60.055
PRIMER_RIGHT_1_TM=59.741
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.3842
PRIMER_LEFT_2_PENALTY=0.304436
PRIMER_RIGHT_2_PENALTY=0.079714
PRIMER_LEFT_2_SEQUENCE=ACAGCTCCCTACTTCTTAGCGA
PRIMER_RIGHT_2_SEQUENCE=GGATGTAGTGTTGTACTGCCCA
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=343,22
PRIMER_LEFT_2_TM=59.696
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=0.3939
PRIMER_LEFT_3_PENALTY=0.134571
PRIMER_RIGHT_3_PENALTY=0.259289
PRIMER_LEFT_3_SEQUENCE=CCCCATTCCTTCTTTAACACAG
PRIMER_RIGHT_3_SEQUENCE=GGTAGCTGAGGATCAGATTCGT
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=191,22
PRIMER_LEFT_3_TM=59.865
PRIMER_RIGHT_3_TM=59.741
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=0.4689
PRIMER_LEFT_4_PENALTY=0.209637
PRIMER_RIGHT_4_PENALTY=0.259289
PRIMER_LEFT_4_SEQUENCE=CAACCCCATTCCTTCTTTAACA
PRIMER_RIGHT_4_SEQUENCE=GGTAGCTGAGGATCAGATTCGT
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=191,22
PRIMER_LEFT_4_TM=60.210
PRIMER_RIGHT_4_TM=59.741
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_04281 Ps_contig_mira-and-tgicl-ass_franssen_32750
SEQUENCE=TCAACGCAGAGTATCGGGGATAACAATCAACCCCATTCCTTCTTTAACACAGCTCCCTACTTCTTAGCGAGTAAGAAACAAAACCAATCTCTCTCTCTCTAGAAAACTCCTCTTTTTTCTCCTTGTCTTTACACCAAAAAAAATGCATAACCCCCATAAGCCCACCGCCCACGAATCTGATCCTCAGCTACCAGTAATCAGAATCCACCACCCGTCCTCCCCTCGCCACCAGTCCGCCGCCACACCCACTCCCACTGCCGGCGCCCGCCGAAAAATCGGCGTAGCCGTTGACCTCTCCGACGAGAGCGCGTACGCTGTACGCTGGGCAGTACAACACTACATCCGTCCCGGTGACGCCGTCATCCTCCTCCACGTCAGCCCTACCAACGTCCTCTTCGGTGCTGACTGGGGTTCCATCGATCTCACCATTAACACAGACCCTAATTACGAAGAAGAAAACATCAACGGCGTCAACAACAACAATCTTAATGAAACCCCGACGCCTAAGCGGAAGCTCGAAGAAGATTACGACGCGTTCACGGCAACGAAATCAGCCGATCTTGCGAAACCGCTTCGTGAAGCTCAAATTCCGTACAAGATCCACATCGTGAAGGACCATGACATGAAGGAGCGTCTTTGTCTTGAGGTTGAGCGTCTTGGTCTTAGTGCTGTTATTATGGGGAGCCGCGGATTCGGTGCGGCGCGTCGCGGTAGTGATGGGAAGCTGGGAAGTGTTAGTGATTACTGTGTTCATCATTGTGTCTGCCCTGTTGTGGTTGTTCGCTATCCGGATGATAAGGATGCTGCTGAGGCTGTTGTTGCTGTCAAAGAGGGTGATGAGGGTGAAGCTGTTATCAAGCCTCTTTCTGTTGCGCATGAACATAAGAAAGAAGATTAGTGGGAGCCAATTCATCACTTCAACCCTGTCTCCGCAGGTTGTACTATTGAGCATGTTCCTGGTTGTTAACTCTGGAAAGATTATGGATGGTTTTTTTATGGCTTGATGATACTAGTTCCTTTAAGCTGTTGGAAGGTTATGTTTGGTTCAGAAGTGGCATGTACTGTTTCATGTAGATGTACAAGTTTGCTGTGTGACCCTTTGGGGTTATTTCCCATTCTGTATGTGGGGGAAATCGAATTATCTTGATGAGCACTTATTTAGCTAGTAGATAAATTCATAGAGCTATGTTGTTGTTGATGGGACTATGTCTGTTTTGAGTTCAGCACATTTACCCGGTGTTAAAAAAGAAAAGAAAAATTAAACTGAAATCTTGTTGATGATAG
TARGET=472,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0931
PRIMER_LEFT_PENALTY=0.079714
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=TGGGCAGTACAACACTACATCC
PRIMER_RIGHT_SEQUENCE=TCGTAATCTTCTTCGAGCTTCC
PRIMER_LEFT=322,22
PRIMER_RIGHT=530,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.1074
PRIMER_LEFT_1_PENALTY=0.079714
PRIMER_RIGHT_1_PENALTY=0.027712
PRIMER_LEFT_1_SEQUENCE=TGGGCAGTACAACACTACATCC
PRIMER_RIGHT_1_SEQUENCE=GGATCTTGTACGGAATTTGAGC
PRIMER_LEFT_1=322,22
PRIMER_RIGHT_1=601,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.972
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.1213
PRIMER_LEFT_2_PENALTY=0.079714
PRIMER_RIGHT_2_PENALTY=0.041553
PRIMER_LEFT_2_SEQUENCE=TGGGCAGTACAACACTACATCC
PRIMER_RIGHT_2_SEQUENCE=CGTCGTAATCTTCTTCGAGCTT
PRIMER_LEFT_2=322,22
PRIMER_RIGHT_2=532,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=60.042
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.2188
PRIMER_LEFT_3_PENALTY=0.079714
PRIMER_RIGHT_3_PENALTY=0.139129
PRIMER_LEFT_3_SEQUENCE=TGGGCAGTACAACACTACATCC
PRIMER_RIGHT_3_SEQUENCE=TGTGGATCTTGTACGGAATTTG
PRIMER_LEFT_3=322,22
PRIMER_RIGHT_3=604,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=59.861
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.3443
PRIMER_LEFT_4_PENALTY=0.330865
PRIMER_RIGHT_4_PENALTY=0.013401
PRIMER_LEFT_4_SEQUENCE=TCCATCGATCTCACCATTAACA
PRIMER_RIGHT_4_SEQUENCE=TCGTAATCTTCTTCGAGCTTCC
PRIMER_LEFT_4=412,22
PRIMER_RIGHT_4=530,22
PRIMER_LEFT_4_TM=60.331
PRIMER_RIGHT_4_TM=59.987
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_04282 Ps_contig_mira-and-tgicl-ass_franssen_32752
SEQUENCE=ATTGTGCAAATAATAAAGTCTGGTCGATTCTTCATAATTAACTGCNACTCATTCATATGATCATATATTTTTTTTCATTCTTTTCATCATAACCTTACCCCCTAGAATTCTCATCGATATATCTTAGGGTTAGGGTTAGTCACCAAACACTATAGTACTAGCTATAGCTACTTAGGTTACGGTTACGGTTACTATTCATTTTTTTTTCTTCATAAGAATTGAAATATATGAAGACATAAAAGGAAACAAGATTCAGATCTATAGAGATAGGGTTTTGGAAAAAGATGAAAAAGAGACAAGTGGTGGTGGTGGTGAAAAGCAACAACAGTTCATCTTCAAGAAATGTTGTACGTTATGGAGAGTGTCAGAAGAATCATGCTGCTACGAGTGGTGGATATGCTGTTGATGGATGTATGGAGTTCATGGCGAGTTCTGGAGATGATGCACTTATATGTGCTGCTTGTGGTTGTCATAGGAACTTCCACAGAAGAAAAGTGGAAAGTGCAGATTGAGATTGTTGGTATTTTGATTAATTTCTTCTAATTTGTGTTTATTGATTTGTTAATTACATGTGTATTGATTGAATTAATTAACTCATTCATGTATTTTTCTTATATTTTTTGTTTCTTTGGGTGTGTTTGTAAGTGTTGATTACCAGAGAAACCATGATTTCATGTAAAAAGAAAAGTAAATTTCTGAACTAATATGTGGCTTGAATTTGTAACTAAANCCGTTCTTATTTTTANCC
TARGET=300,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1838
PRIMER_LEFT_PENALTY=1.065643
PRIMER_RIGHT_PENALTY=0.118120
PRIMER_LEFT_SEQUENCE=AGGGTTAGGGTTAGTCACCAAA
PRIMER_RIGHT_SEQUENCE=CATCAACAGCATATCCACCACT
PRIMER_LEFT=125,22
PRIMER_RIGHT=407,22
PRIMER_LEFT_TM=58.934
PRIMER_RIGHT_TM=59.882
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=1.2870
PRIMER_LEFT_1_PENALTY=1.065643
PRIMER_RIGHT_1_PENALTY=0.221368
PRIMER_LEFT_1_SEQUENCE=AGGGTTAGGGTTAGTCACCAAA
PRIMER_RIGHT_1_SEQUENCE=CATCCATCAACAGCATATCCAC
PRIMER_LEFT_1=125,22
PRIMER_RIGHT_1=411,22
PRIMER_LEFT_1_TM=58.934
PRIMER_RIGHT_1_TM=60.221
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=1.2870
PRIMER_LEFT_2_PENALTY=1.065643
PRIMER_RIGHT_2_PENALTY=0.221368
PRIMER_LEFT_2_SEQUENCE=AGGGTTAGGGTTAGTCACCAAA
PRIMER_RIGHT_2_SEQUENCE=TCCATACATCCATCAACAGCAT
PRIMER_LEFT_2=125,22
PRIMER_RIGHT_2=417,22
PRIMER_LEFT_2_TM=58.934
PRIMER_RIGHT_2_TM=60.221
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=1.2870
PRIMER_LEFT_3_PENALTY=1.065643
PRIMER_RIGHT_3_PENALTY=0.221368
PRIMER_LEFT_3_SEQUENCE=AGGGTTAGGGTTAGTCACCAAA
PRIMER_RIGHT_3_SEQUENCE=ACATCCATCAACAGCATATCCA
PRIMER_LEFT_3=125,22
PRIMER_RIGHT_3=412,22
PRIMER_LEFT_3_TM=58.934
PRIMER_RIGHT_3_TM=60.221
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=1.5439
PRIMER_LEFT_4_PENALTY=1.065643
PRIMER_RIGHT_4_PENALTY=0.478252
PRIMER_LEFT_4_SEQUENCE=AGGGTTAGGGTTAGTCACCAAA
PRIMER_RIGHT_4_SEQUENCE=AACAGCATATCCACCACTCGTA
PRIMER_LEFT_4=125,22
PRIMER_RIGHT_4=403,22
PRIMER_LEFT_4_TM=58.934
PRIMER_RIGHT_4_TM=59.522
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=279
=
PRIMER_SEQUENCE_ID=SSR_04283 Ps_contig_mira-and-tgicl-ass_franssen_32759
SEQUENCE=CGGAAACGGTAAAACGAAACGTAAACAAACTCCTCCTYYYTCTTYTTCTTCTTCTTCATCTTCTTCTTCTTCTTCTTTCATCCATCTAGGTTTTTACAAACCAAATTCTCGTGACTTCAGTTGCATCCATACCCAACTCAGAAAAACCAAAAACCAATAAACAATAAACAAGAAAACAAATCAAATCTTTAAGAAACTCTCAAGAGTGGTGATAAGTTGTAATCTACTATTTAGTAATTAAACTGAATTGAAGTGTTTCATAGGAAAACCTGTCTGAAGTCTTTGGAATCTTTGCAAATTTCTTGGATCTCTGAGCATGACCGGGAACCAAGAGCAGTTTGAGATCAAGTTTAGGTTGACAGATGGTTCTGATATTGGCCCGAAAAGTTTTCCAGCTGCTACTAGTATTGCAACGTTGAAAGAAAGTATTCTTGCTCAATGGCCAAAAGATAAGGAGAATGGGCCGAAGACTATAAAAGATTTGAAGTTAATTAGCGCAGGAAAAATATTGGAGAACAGCAGCACAGTTGGGGAATGCCAGAGTCAGAATCCTTTGTGTGATACACCCGGTACAGTTACAACAATGCATGTGGTTGTGCAACCACCTACTACAGACAAAGAGAAGAAGGCCGCAAGCAACACAACACAGCAAAAGTGTGGTTGTGTTATTTTATAGCATAAGATCGAATAGCAAAAAATATCATCATCTTTCTTAGGTGGTTCGGTTAGATACCCTTCACTTTCACACGTAATCTGCGGCTTTCAAAGATTTCCTCATACACAAATTATTTGGCTGCATACCTCATATCTTTACTACAGGAAAAGAGAATTGTGGTTCTCTGTGACAGACAAAACTCTTGTACAATTTTCATAGATGTTTTATTTCTGGTCATAGTCCTCATAAGCCACTTACTGAATTGAACTCTCTCTTCAAGTTTACCATTGTTGTATTGTATCTGATGTGTATTTTGAGATTTTTCTTATACCTGTGCCTGGTGCAAAATGTTTGCAGACTTAACTGTGTTGAATGTTTGAATGTGTCTTTTAGTTTTGGGTTNATTTGTTTATTATTGTNNTGTTCGTTTAGTTACTAANTAAAAAACT
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04284 Ps_contig_mira-and-tgicl-ass_franssen_32759
SEQUENCE=CGGAAACGGTAAAACGAAACGTAAACAAACTCCTCCTYYYTCTTYTTCTTCTTCTTCATCTTCTTCTTCTTCTTCTTTCATCCATCTAGGTTTTTACAAACCAAATTCTCGTGACTTCAGTTGCATCCATACCCAACTCAGAAAAACCAAAAACCAATAAACAATAAACAAGAAAACAAATCAAATCTTTAAGAAACTCTCAAGAGTGGTGATAAGTTGTAATCTACTATTTAGTAATTAAACTGAATTGAAGTGTTTCATAGGAAAACCTGTCTGAAGTCTTTGGAATCTTTGCAAATTTCTTGGATCTCTGAGCATGACCGGGAACCAAGAGCAGTTTGAGATCAAGTTTAGGTTGACAGATGGTTCTGATATTGGCCCGAAAAGTTTTCCAGCTGCTACTAGTATTGCAACGTTGAAAGAAAGTATTCTTGCTCAATGGCCAAAAGATAAGGAGAATGGGCCGAAGACTATAAAAGATTTGAAGTTAATTAGCGCAGGAAAAATATTGGAGAACAGCAGCACAGTTGGGGAATGCCAGAGTCAGAATCCTTTGTGTGATACACCCGGTACAGTTACAACAATGCATGTGGTTGTGCAACCACCTACTACAGACAAAGAGAAGAAGGCCGCAAGCAACACAACACAGCAAAAGTGTGGTTGTGTTATTTTATAGCATAAGATCGAATAGCAAAAAATATCATCATCTTTCTTAGGTGGTTCGGTTAGATACCCTTCACTTTCACACGTAATCTGCGGCTTTCAAAGATTTCCTCATACACAAATTATTTGGCTGCATACCTCATATCTTTACTACAGGAAAAGAGAATTGTGGTTCTCTGTGACAGACAAAACTCTTGTACAATTTTCATAGATGTTTTATTTCTGGTCATAGTCCTCATAAGCCACTTACTGAATTGAACTCTCTCTTCAAGTTTACCATTGTTGTATTGTATCTGATGTGTATTTTGAGATTTTTCTTATACCTGTGCCTGGTGCAAAATGTTTGCAGACTTAACTGTGTTGAATGTTTGAATGTGTCTTTTAGTTTTGGGTTNATTTGTTTATTATTGTNNTGTTCGTTTAGTTACTAANTAAAAAACT
TARGET=59,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04285 Ps_contig_mira-and-tgicl-ass_franssen_32760
SEQUENCE=TGGTAGAAAACCAAAATAAACATGTAAGTAACAAATAAGAACAAGTAGTAAGTTTCTTACATGATAGGAGTTTTTGGTAATTGTCAATTTAGAGATTATACGAGTTTGAGATGATATTACATGCCAGTGCCATACATACTATCTACTGGGCAGTGTATACACTTCCACTCCAATTCCATTTCATTTTTGTTTCCTTTTGCTAGATTAATGAGGCGACAAAGTAACAAAAGGGCTCACATGTCAGCAACCAATTTTTCCTCGCCATCTATGGATTCGGCAAGGGTTCCCCGAGTCCACTGCTTAAGCATCTCTAATGCAGCCTTGGGCTGGTCCATTGGAACCATGTGACCCGCATCATGGACCTTAAGAAAACTCAGAGGACCATAGCTCTTCAATAATCCAGCTTGTGATCCATTAACAACAAAAGGAACATCAGACGAAGCTACAAATTCTTTCTGACCAGACCATTTCATGGCATGAACCCATCTTGAATTACCAAGCCAGTTGCATATCAAATCATATTCCCCAGCATAAATAAGTAAATTGATTCCATCCTCAAGAAGGAGAGGAATACCAACTTCCAGATTCCTCATCCAGTCCACCAACATAGCCATATAAACTTCAGTGCTACAAGAAACAAAACGAATTTTGCCAACCCCTAGTGAATCCCGAACCGATTCTTGGTTCAGGAACTTCTCCATGTTTGAGAAATCATAACAGAGGCTCCCCTCACACTTCTTTCTGATATCATAGTAATTTACACCTCCGGCATGTAACATGATGTCACTGAATATGAGATTACAGGCTACATTTGCAGTCAGGCAGGAAACCTTACCATTGGTCCCACATAGCTTTATTGCCAATTCACAGGCTGGAACCAATACTTTGCCAAGGAGGTCATGTGTAGTCTGTGTAATTATGCCCATGTCTAGTGCATAATCTGGGTAAGCTTTATACTGAATTGCAGGATTAGTAAGCCCATTACCAATGGCCAATCCCTTCAAATTTATATGAATTCCTTCATTAGCTTGGTTTCCCTGATGGATACGAGAGGCTAAAGCAGGAATATAGTGCCCGGCATACGATTCACCTGTAATAAAAAAATCATTCTTTGCATATTGGGGATGCTCTACAAAGAAGGCCTGCAAAAAGTCATACAGGTCATTGCTAACACCCTTTTCATTATGACGAATGTCACGCAAATCAGTACTGTAGCTAAAGCCGGTTCCAGTTGGTTGATCAACATACAAAAGGTTTGACACCTTGTCCCAACCATACTCATTCCACACAAGAGACAAATTGTGATTAATTTTGAAAGGGCCATTTTCATAAAACAAAGCCAATTCACTGCTACAGCCAGGACCTCCAGTCAACCAAATCACAACAGGATCTTCCTTGCGGTTGCGTGATTCAAAGAAAAAGTAAAACATCCTTGCGGCATGGGAATGTTGAATGGGATAATAACCGGCATAATGACCAAAATCCTCAACGGAAACATCATCATCGTCATCGCCAACAAGATTAGGAAACTTGAGGCGCTTCTCAACGATTTTGCCACTGAAATCTAATGAAGATGAATTTGCAGCAGCAACGATGTTGAGGTTGACTTCCGGGAACAAATTAAGGTCTCTCACTAGCTTCTTAGCTTCTAGCGTGAGGCGAAGATCGGCGGCGGCGGGAGAAGCAATTGATGAGCAGAAGTAAAGGAAGAGGAAGATTGACGTGGAAGAAAGCCCCATTTTGTAACGTATGGCAGATAGATACTGCACTGCACTTGACCC
TARGET=1667,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2027
PRIMER_LEFT_PENALTY=0.093228
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=GAATGGGATAATAACCGGCATA
PRIMER_RIGHT_SEQUENCE=TTTCTTCCACGTCAATCTTCCT
PRIMER_LEFT=1450,22
PRIMER_RIGHT=1731,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.2027
PRIMER_LEFT_1_PENALTY=0.093228
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=GAATGGGATAATAACCGGCATA
PRIMER_RIGHT_1_SEQUENCE=CTTTCTTCCACGTCAATCTTCC
PRIMER_LEFT_1=1450,22
PRIMER_RIGHT_1=1732,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.2027
PRIMER_LEFT_2_PENALTY=0.093228
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=GGGATAATAACCGGCATAATGA
PRIMER_RIGHT_2_SEQUENCE=TTTCTTCCACGTCAATCTTCCT
PRIMER_LEFT_2=1454,22
PRIMER_RIGHT_2=1731,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.2177
PRIMER_LEFT_3_PENALTY=0.108177
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=ACTTCCGGGAACAAATTAAGGT
PRIMER_RIGHT_3_SEQUENCE=TTTCTTCCACGTCAATCTTCCT
PRIMER_LEFT_3=1605,22
PRIMER_RIGHT_3=1731,22
PRIMER_LEFT_3_TM=60.108
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=0.2177
PRIMER_LEFT_4_PENALTY=0.108177
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=ACTTCCGGGAACAAATTAAGGT
PRIMER_RIGHT_4_SEQUENCE=CTTTCTTCCACGTCAATCTTCC
PRIMER_LEFT_4=1605,22
PRIMER_RIGHT_4=1732,22
PRIMER_LEFT_4_TM=60.108
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_04286 Ps_contig_mira-and-tgicl-ass_franssen_32763
SEQUENCE=TTCCCATTATTTAACTTTCACTGCCTTTCAGGTTTCAAGTCATTCATTCTGTTCACTTTTCTTTGAAAGAATTTGAGTATGATTTTACATGCAATAGTTAAATGAAATTCTCTTGGATAATATATACAACGAAATAAATTATGGAAGAGAAGGTGTGAGAGAAGGCTTTGCAACCTCTAGTGCTCCACAGTCACTAATATAAATTGTGGTAAGTGGCCTGTTCCATATGGCGCGTGCATTCTGAGCCCTTTCGTCTCCGAAAAATTCTGCCAAGTCATTAAATTGGCGAATTCGTTCACCTGGTTGGTAAGTTTGAGTAGAAGCTATGGTTGTGATGACATCCAACCCTTCAAGCACCGCTCCAAAGACAATATTCTTGTTATCAAGCTCAGGACAAGGTCCAGGTCCAGTGGTGATTAAGAATTCAACGTTCCGGTATTCAGGATCAAACTTAATCTCATCGTCATCATCATTTTCAGATAGGGGGAGTGAAACGACGCCGGCCCGGGAATGTGTCAATGCAAATGCTTTGGGGTCGACTGTTTCAATGTTGCGGGGGAGGTGGTTAGGAGGGCGGACATCGCCTCTTTCAGGACGACCCTGATGTCCGGCAAGAAAGTACTGACCTGGAAAAACCTTGTGGATGACTGTGTTTTTGTAGGAAGAGGAATTAGGGTTAGAATTGGAAGCGGAAATGCAGAGGGATTTGAAATTTGAGACTGTGCGAGGAACGAGGTTACCGTAGAGGCCAATGATGACGCGTCCGAGTGAGGTGGAATCTGAGCATAGAGTGGAAATGTTGTCTCCTAGGGTGCGATTTGGGAGGTAATAATTGGGGCAGAGGCTGAATTCCATGAAGACGCGGTCGGTGATGGTGGGGTTTGGTGGTTGTGTTGGTGAGGCGGAAGAGGAAAGGGTGGGGAAGGAAAGAGTGGCGGTGGATGTGAGGAGGAGTGAGCGGCGGGTGAGGTTGGATTTGGGAAATGAGTGGTGGAGAGAGGGAAGAGGTGGTGGTGGTTGTGTTAAGGAGGAGGCCATTGTGGTGGAGGAGTAGTCAGGTTTTTCATTAYNCNGARGAGAACTTGGAGGAGCTAATCAGTGCGGGGTCCACCTTAGATTAGATAAATCTCGTGAAAACTTGCCAACAAGGCCTCATTAGTATTCAAGT
TARGET=1007,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04287 Ps_contig_mira-and-tgicl-ass_franssen_32766
SEQUENCE=AAAAAACACCATGGTGATCAAATATCAAACCCAACATTGTTGTTTTGTTGGACAAAAAAACTTCCTGAGCACAATGGCATATCATAATACACCTCAATGTATGATAATAATACATATAAAATTGGATATAACAAGACATATATCTACTAAGATTGCATCTGAAAGGATGTGGTGAATTGATCTTCTCTAACAATGGTTTTTTTTATCAGATGCTTCAGCAAGCATCAGTCAACCCAATGGTTTTAAATTTTAAGTTTACAAATCTTCAAATAGTTTGCAACATCTCTAGGCCAAATATTGACAATTATTTTGAAGAAGCTGCTATACTGGTGATTTCCACAATTACGAAAAACACAATAGAGGCAAATTAGCCCCTGTATATACCGGAAGGCTCTGCATTCTGAACAATCCAAGGATGTTCTAGCAGTTTGTGCAATGGTAGTCGTTGAGAGGAATCCTTGACCAGCATCTGGCTGATTAGGTCTTTCGCAGCAGAAGAAACAATTGGTTTAGGAGGGAACTTGAGATCCACTTGTATTATCCTTCTATAAGTATCAGAATGTTCTTTAGCCTCAAAAGGGGGGACTCCATAAAGAAACTCATAGCAAAGAACGCCAAGGCTCCATATATCCACGCTTGCATCATGTTCTACACTCTCCACCATCTCGGGTGGAAGGTAATCAAGTGTGCCGCACATGGTCCTCCTGCGGTTGAATGTGTGAACTGACCATCCAAAATCAGCTATCTTCAATTCACCCTGTGCACCAATAAGAAGGTTCTCAGGTTTAATGTCCCTATGAATTACATGCTTTCCATGGCAATATATAAGGGCTCGGGCCAATGACGCAACATAAGTAGCTGCACGTCTTTCACTAAAATATTTGCATTTCTGCAATTCCTTGTAAAGTTCACCTTTAGGTGCATACTCTAAAATCAAATAAACTCGTTTCTGATCATAAAAGTATCCATAGAGTCGCAAGATATGTGGATGTCGAAGATGACTCTGTATTTCAACTTCTCGACGAAGCTGATGCTCAACTTGTGATTGTTGCAATTGAGTCTTAAAGAGTACTTTCAGAGCAATAATATGATTACTAGTTTTTTCTCTGGCCAAATAGACGTGGCCGAATTTCCCCCTTCCAAGAGGTTTCCCTATATCGAAATCATTAAGCGTCCATCTCTTTTGCTCCTGAGCTGAACCTGAATACTCCGAAGAAGCCGTGTGTGGTTGAGGGTGAGGCTGAGACTCGGTTACCACGGCCATGAGAGAGAGAGGTTTTAGAGAGAGAAAGGGTTTGTTGAACGGAGAAATGGAAGAAGACGGTGATTTGAAATCAGCTCCTTCYCTTCTTTCTTTCTT
TARGET=1265,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04288 Ps_contig_mira-and-tgicl-ass_franssen_32766
SEQUENCE=AAAAAACACCATGGTGATCAAATATCAAACCCAACATTGTTGTTTTGTTGGACAAAAAAACTTCCTGAGCACAATGGCATATCATAATACACCTCAATGTATGATAATAATACATATAAAATTGGATATAACAAGACATATATCTACTAAGATTGCATCTGAAAGGATGTGGTGAATTGATCTTCTCTAACAATGGTTTTTTTTATCAGATGCTTCAGCAAGCATCAGTCAACCCAATGGTTTTAAATTTTAAGTTTACAAATCTTCAAATAGTTTGCAACATCTCTAGGCCAAATATTGACAATTATTTTGAAGAAGCTGCTATACTGGTGATTTCCACAATTACGAAAAACACAATAGAGGCAAATTAGCCCCTGTATATACCGGAAGGCTCTGCATTCTGAACAATCCAAGGATGTTCTAGCAGTTTGTGCAATGGTAGTCGTTGAGAGGAATCCTTGACCAGCATCTGGCTGATTAGGTCTTTCGCAGCAGAAGAAACAATTGGTTTAGGAGGGAACTTGAGATCCACTTGTATTATCCTTCTATAAGTATCAGAATGTTCTTTAGCCTCAAAAGGGGGGACTCCATAAAGAAACTCATAGCAAAGAACGCCAAGGCTCCATATATCCACGCTTGCATCATGTTCTACACTCTCCACCATCTCGGGTGGAAGGTAATCAAGTGTGCCGCACATGGTCCTCCTGCGGTTGAATGTGTGAACTGACCATCCAAAATCAGCTATCTTCAATTCACCCTGTGCACCAATAAGAAGGTTCTCAGGTTTAATGTCCCTATGAATTACATGCTTTCCATGGCAATATATAAGGGCTCGGGCCAATGACGCAACATAAGTAGCTGCACGTCTTTCACTAAAATATTTGCATTTCTGCAATTCCTTGTAAAGTTCACCTTTAGGTGCATACTCTAAAATCAAATAAACTCGTTTCTGATCATAAAAGTATCCATAGAGTCGCAAGATATGTGGATGTCGAAGATGACTCTGTATTTCAACTTCTCGACGAAGCTGATGCTCAACTTGTGATTGTTGCAATTGAGTCTTAAAGAGTACTTTCAGAGCAATAATATGATTACTAGTTTTTTCTCTGGCCAAATAGACGTGGCCGAATTTCCCCCTTCCAAGAGGTTTCCCTATATCGAAATCATTAAGCGTCCATCTCTTTTGCTCCTGAGCTGAACCTGAATACTCCGAAGAAGCCGTGTGTGGTTGAGGGTGAGGCTGAGACTCGGTTACCACGGCCATGAGAGAGAGAGGTTTTAGAGAGAGAAAGGGTTTGTTGAACGGAGAAATGGAAGAAGACGGTGATTTGAAATCAGCTCCTTCYCTTCTTTCTTTCTT
TARGET=1348,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04289 Ps_contig_mira-and-tgicl-ass_franssen_32767
SEQUENCE=ACGTAAAGTGATCCTTACTTTTAATTTTATTTACATAGTTAAAGCTCTGATGTTTCTATTATACAAAGCATACCATGATGTAATCAGTATCAATATAAAGTAAACTATTATCTTGAATAACGGTCCACAAGTGCTTTAGCTTTATCTGGTGCAAAGAACCTAGCTTGTATGAACATCCTCTCTGCATCTCCAATGTTTTTGCTTTCTTTTAAGATGATTCCCTTAGCTAAGTATCCACGGAAGTCATCAGGATGAGTAGATATGAGTTGATTGTAAACAGCTATAGCATCACCAACATGGCCCCAGTCTGAATAGGCTTTCCCTAGAAGTAATTCAACTTGAACTGGATCCAGCTTCTGTGAGTCTGTTGGGCTGCTATCAGCTTTGTTGCTCAAGTCTTTTGAGCTCAGGCGGTCGCGACAAGCCAAAAGTAATTGAACAGCCTCATCTGGCTTCTTAGCAGCAAGTAATGAATTGGTAAGGCCACGCAGAACTTCAAAATTGACATCTTCAGATACCTTTGCCGAACTTTTATATGCAGCAACACTCCCATCATAATCTTTAAGTTCATACTTAACTTCTCCAAGCAAACGAAAAGCATCAGCATCATTTGGTTTTTCCTTCGTCAAATCATCGAGTAGAGAAGCAGCTTTTGAATATTCCCCTAGTTCTGCCAATGTTACTGCAGCACCTTCGATAGCTGCTGGATCTCTTGGGGAGTTACTTAATGTCGCTTCAAATTCCTTAAGCCTACTCTGAAGAGCAGCTTTATCCTTCTCTGACAACTTATCATTGACCACAGCAGTGTCTTCGTTGGGACTTCCAGATGGAAGAAAATCTCCTAAAGCAAAAACCAGACCAAAGACAGCAACAGCTACACCTATTCCAACCTTGGTACCAAAACCAATTGTCTTGTTGTCTGAAGAAATAGGTGTGTCATAATCAATAGCTCCAAATTCTTTTTTCTCTTCTGCCTTCTTTTTCTCTAGTGCTGACCTTCTAACTGCTTTTAGGCGTTCTTCAAAATCAATGTCCGAAGAAATGCTTCTGGATTTGCCATCAACTTTTGAAGTTAAACGCGGTGCCTGAGATTGTTTAGTTGATGATTCCCTTGGGTTTCGCCGCGATTGCGAACCCTTTTTGTTAGGTTTGTTGGTCTTATCTCCGAAGCCACCGCCAGCAACAGAACGGTTATTTGGATTTGAATTGGAGCAATTAATCATTCGAAACGGAGAGAAAGCATTGTTGTTGTTGTTGCTTCTTCTGATTGACATTGCATGATTAATGGTTGAAGTCGTCGACATCATTGACATGTTGAATGAGGGACTGAATATGAGTATCAACTTCGTCTTCTTCAACACACCAATTTATGAACGATAAGGTTTCCCTCTGTAATAATAGCTCTAATACAAATCACTAATTAACGCTCTTAACCACTCGTTTCTATATTTTCAAATTCTTCTATTTTATCAAATT
TARGET=1243,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0072
PRIMER_LEFT_PENALTY=0.003602
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=GATTGCGAACCCTTTTTGTTAG
PRIMER_RIGHT_SEQUENCE=CGACGACTTCAACCATTAATCA
PRIMER_LEFT=1125,22
PRIMER_RIGHT=1299,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=175
PRIMER_PAIR_PENALTY_1=0.0968
PRIMER_LEFT_1_PENALTY=0.093228
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=CGAACCCTTTTTGTTAGGTTTG
PRIMER_RIGHT_1_SEQUENCE=CGACGACTTCAACCATTAATCA
PRIMER_LEFT_1=1130,22
PRIMER_RIGHT_1=1299,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.2148
PRIMER_LEFT_2_PENALTY=0.211181
PRIMER_RIGHT_2_PENALTY=0.003589
PRIMER_LEFT_2_SEQUENCE=GAAGAAATGCTTCTGGATTTGC
PRIMER_RIGHT_2_SEQUENCE=CGACGACTTCAACCATTAATCA
PRIMER_LEFT_2=1035,22
PRIMER_RIGHT_2=1299,22
PRIMER_LEFT_2_TM=60.211
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.2214
PRIMER_LEFT_3_PENALTY=0.217855
PRIMER_RIGHT_3_PENALTY=0.003589
PRIMER_LEFT_3_SEQUENCE=CCCTTTTTGTTAGGTTTGTTGG
PRIMER_RIGHT_3_SEQUENCE=CGACGACTTCAACCATTAATCA
PRIMER_LEFT_3=1134,22
PRIMER_RIGHT_3=1299,22
PRIMER_LEFT_3_TM=59.782
PRIMER_RIGHT_3_TM=59.996
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.2476
PRIMER_LEFT_4_PENALTY=0.243965
PRIMER_RIGHT_4_PENALTY=0.003589
PRIMER_LEFT_4_SEQUENCE=GCAACAGAACGGTTATTTGGAT
PRIMER_RIGHT_4_SEQUENCE=CGACGACTTCAACCATTAATCA
PRIMER_LEFT_4=1179,22
PRIMER_RIGHT_4=1299,22
PRIMER_LEFT_4_TM=60.244
PRIMER_RIGHT_4_TM=59.996
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_04290 Ps_contig_mira-and-tgicl-ass_franssen_32768
SEQUENCE=CTAATTTTTAATTAATTAATTAAAGAAATACACAAGACAATTGCAGTATTTTGAGCAGTATCAGCAGAGAGTGAGTGCACTGATTGGAGATGAAGAAACAGTGAGATTGGTGAATGAAGCTTTAATTTTGATGACTTTAGGTGGAAATGATTTTGTGAACAACTATTTTCTTGTTCCTTTTTCTGCAAGATCACGACAATTTCGACTACCAGATTATGTTGTATACCTCATTTCTGAGTATCGTAAGATTCTAGTGAACCTTTATAATTTAGGAGCAAGAAGAGTGTTAGTAACTGGTACTGGTCCTATAGGCTGTGCACCAGCAGAACTAGCTCAGCATAGCAGAAATGGTGAATGTTATGGAGAACTTCTGGAAGCTGCTAATTTGTTCAATCCTCAACTTGCTCAGTTATTATCTCAACTTAACTCTGAAATTGGTGCTGATGTCTTTATTTCTGCAAATGCCTTCGCAATGAATATGGATTTCGTTAGCGATCCTCAAGCATACGGATTTGCTACATCAAAAGTTGCATGTTGTGGTCAAGGACCCTACAATGGGATTGGACTCTGTACTCCAGCATCAAACCTATGTCCAGACAGAGATGCAAATGTATTTTGGGATCCCTTCCATCCAAGTGACAGAGCAAACAGACTGATTGTAGACAGGTTCATGGTAGGATCCAGTGAGTACATGCATCCAATGAATCTCAGTACCATTATGCTTTTGGATTCAAAGACCTAATTAATACATTAATATATACCTTAATGTTTTCAGCACAATATTATGTTGTATTATATATTGAGTGCTTAGACTATATTATTATTTTTACTATGTGTGCAAGAATATTGTGCTGTGCTGATGATTGTAACACAAGGAAACCAATAAATTGTTACTATTTCTACGTANATCAATCTTCTAGCCGCTTTAG
TARGET=8,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04291 Ps_contig_mira-and-tgicl-ass_franssen_32776
SEQUENCE=GGGTACGANAGTTTCTTTCGTAAGATATAGCAAGACACCAACCTTAACCCAATCAAACTAAAGAAAAGTTTAGCAACTTCCTCCCAAAGATGGCTGTTTCCGGCAAAACAAGAACGAGGGCTACTTGTCCATGTCTCAACGGTTCAGACCGGGTTGCACGTGTTCGAACCGACCCGGTTCGGTTCGTTGTGCGCGTCATGGGTATGTTGTACCAGGGGAAAAGTTTAAGAAGCGCGTTGCGAGCAAAGAGATTTTGAGAAGAGCTTTAATGCCACCGCCGAAACGTTTAGCACTTAGATGGTTGAATTTCAAACCAACACCAAGTAGACTCTCTATCATGTCTTTGGCTTCATGATTTTTCTCTCTCCTAATTCATTTGTTTTTTTTTTGTTGATGNTTGTACAATTTAGGTTACATCCAGTTGATTAGNTGATGATGATGATGTATGTATTATTTAATTTTTAA
TARGET=431,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04292 Ps_contig_mira-and-tgicl-ass_franssen_32777
SEQUENCE=GAGAATCATAAACCTTGAATTNTAAAGAAGTAAATTCAAATTAATGTTTTTATGTATATTCTCAAGTATATAACCAACTAAATTTGTATGAAACATAGTTCCTTACAACTTATTACCAAATGGCTTATTTAAAAACATCCAAATTCCTCATAGAATGAGAAAATCAGATCCAGACAGATTACAGATTCTATATCGAATTCAATTATCCATTTCTTCAATCCTTCTCCTCCCTATAAATCAAAAGGATAGCCGTTCTTGCGCCTACTTCTTGATCTTTCGATGTTTCTTCGGTCCCGGAACTTCTAACCTACCAACAGAACAACCACACCTAGCTCTCAGCACAAGCACAGCTCTTCCATTAATCGGCGCCATCTTCTTGAGTTCTGCTTCAAGTTCCTTATGATGATCGCGGGGCAATTCGAATAAACCCTTCTTACAAACACCGATATCTTTCGCAAAAGAAGGCGAATTCTTCACACAATCTTCAGTCTCTAATTGAATATCAAACTCTGCAGCTTTCTTCAAACCCTTACAATTTCGATTACCGCATTTGTTAGTTGTGCAAACAACAACACCCCAAGTAGCAAACACAGCACAAGCAACACTCAATCCTATAGATATATAAATCGAAGGCGCAATCAAAAACTCTTCCTTAATCAACAACAACACCATGCTTGTAATCTGAATTGAATTAACCACTAAAAATTCTATGTAAGGGAAGAACAAATAGCCACAAGATAAAACAACAGCAACGAAGATAACAACATCAACTATAGCTGATCTAGATCGATCACAAGGTGGAATCTGAAGAGAAATCGATGATGAACTATTATAATTCCTCCGATTCCTTTGAAAGTTATTGCTTTGCCTAGATTTCGAACCGAAATCAATCGAATTCGCCATGGGTGGATTTGGTGGTTGATCAACTGACTTACAAAGAGAGATTGAGCTATGGAAATAGACCATTTCAAAAACACCGCAAAATTAAAAGAAAATCTAAGAAAAATCGNAATTTTTAGATCAAACCCACCAGTAATTAACCGAGATTCAAACGACCCAGAATTCAAAATCTCGAAAAGGTTGTAACTTTGAAGTGATCTAAGCACAGAAAAATATCAAATTCTGTCGGTGCCCAGATGGATCAAATTCCCAAAATGAGATTTGTTTGCTTCTTGTTCGAATTTAAATTCAATATGTGATGAGATGATGATTCAATCAAAGAGAGATAAACAAGATTGAGAAATTGAAGAGGTGAAAAATTAGTGTGATGGAAATGGGTTTTAGGGGTTGAAAAGTGAGTTGATGAAGAAGCTAAGAAGACAAAGAAGGGTCATCATTCACTAATTTGTTATCATCATATCATATGATAATAATAATACTAATAATATTCATAGTNTATGTGAAATGAAGTCTT
TARGET=1367,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04293 Ps_contig_mira-and-tgicl-ass_franssen_32779
SEQUENCE=GGATGCGGGAAGAAGCGAGGAAGTTGAAGATGCTGATGATGATGAATTTGGGGATGTGTATCACTCGCCACGCGTTTTCAAGTTGAATTTTGCGGAGATGGAGAAGAGGTTCAAGGTTTATATATACCCAGATGGGGATTCTAAAACTTTTTATCAGACTCCGAGGAAGCTTACGGGAAAGTATGCTAGTGAGGGGTATTTCTTTCAGAATATTAGGGAGAGTCGTTTTCGGACTCTTGATCCTGATCAGGCTCATCTCTTTTTCATTCCCATTTCATGTCACAAGATGCGTGGCAAGGGCACATCTTATGAGAATATGACCATAATTGTACAAAATTACGTGGAGAGCTTGATATCCAAGTATCCTTATTGGAACAGAACATTGGGTGCCGATCATTTCTTTGTCACTTGTCATGACGTTGGTGTGAGGGCAACAGAAGGACTTCCATTACTTGTGAAGAATTCCATTCGAGCAGTGTGTTCCCCCAGCTATGATGTTGGATTCATTCCACACAAAGATGTTGCTCTCCCTCAAGTGCTACAGCCATTTGCTCTTCCAGCTGGAGGGAATGATGTAGAAAATAGGACTAGTCTTGGATTTTGGGCTGGTCATCGTAACTCTAAAATTAGAGTTATTCTAGCGCGTGTATGGGAAAATGACACAGAACTTGACATTTCAAATAACCGAATTAGTAGGGCTACTGGGCATCTAGTGTACCAGAAAAGATTTTACAGGAGTAAGTTTTGTATATGCCCTGGTGGATCACAGGTTAACAGTGCTCGAATAGCAGACTCTATTCATTATGGATGCATACCTGTGATATTATCAAATTACTATGACCTTCCTTTTAATGATATTCTTGACTGGAGAAAATTTGCTGTTGTACTCAAGGAAAGTGATGTATATCAGCTTAAACAAATCCTCAAAAAAATATCACAGGCCGAGTTTGTTGTACTTCATAATAATTTAGTCAAGATTCAGAAACACTTCCAGTGGAATTCACCTCCAGTGAGATATGATGCTTTCCATATGGTTATGTATAACCTCTGGTTACGCCATCACACCATCCAATACTGATTCTAAGTGCTCGTCTGTTTTTGCGGATCAATGTCCAAGTGCACGCCAGGATCAAACCTACAGTTGTAGCTTCTGTTAAATCGCGGTCAATTGGCATACGAATGCCAGTTGAGTGTATCTAGAAGTGAAACTAAACAAGCCCCAAAGACTCACTCTTATCCTGTATACACCCTACTTAGCAATCCAGAGAGGCACTGTAAATAACTACATTACTGTTTTATATGTAACGGCTAATTTTTNCAATATTTCTTGCACTCCTA
TARGET=34,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0933
PRIMER_LEFT_PENALTY=0.018835
PRIMER_RIGHT_PENALTY=0.074466
PRIMER_LEFT_SEQUENCE=AGAAGCGAGGAAGTTGAAGATG
PRIMER_RIGHT_SEQUENCE=ATCAGGATCAAGAGTCCGAAAA
PRIMER_LEFT=10,22
PRIMER_RIGHT=246,22
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.1624
PRIMER_LEFT_1_PENALTY=0.018835
PRIMER_RIGHT_1_PENALTY=0.143555
PRIMER_LEFT_1_SEQUENCE=AGAAGCGAGGAAGTTGAAGATG
PRIMER_RIGHT_1_SEQUENCE=GCTTCCTCGGAGTCTGATAAAA
PRIMER_LEFT_1=10,22
PRIMER_RIGHT_1=169,22
PRIMER_LEFT_1_TM=60.019
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.2048
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.074466
PRIMER_LEFT_2_SEQUENCE=GAAGAAGCGAGGAAGTTGAAGA
PRIMER_RIGHT_2_SEQUENCE=ATCAGGATCAAGAGTCCGAAAA
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.2462
PRIMER_LEFT_3_PENALTY=0.018835
PRIMER_RIGHT_3_PENALTY=0.227356
PRIMER_LEFT_3_SEQUENCE=AGAAGCGAGGAAGTTGAAGATG
PRIMER_RIGHT_3_SEQUENCE=CTTGTGACATGAAATGGGAATG
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=285,22
PRIMER_LEFT_3_TM=60.019
PRIMER_RIGHT_3_TM=60.227
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.2619
PRIMER_LEFT_4_PENALTY=0.018835
PRIMER_RIGHT_4_PENALTY=0.243031
PRIMER_LEFT_4_SEQUENCE=AGAAGCGAGGAAGTTGAAGATG
PRIMER_RIGHT_4_SEQUENCE=GATCAAGAGTCCGAAAACGACT
PRIMER_LEFT_4=10,22
PRIMER_RIGHT_4=241,22
PRIMER_LEFT_4_TM=60.019
PRIMER_RIGHT_4_TM=59.757
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_04294 Ps_contig_mira-and-tgicl-ass_franssen_32780
SEQUENCE=ATCAAGTCCAGCAGTCGGTTCGTCATTTACTAAGAGAACCTCTGGTTCTAATGAATCCTTTGTGGTGTCACAAATAATAGATCGAGCTAAAGCAACTCGCTTTTTCATTCCGCCTGATAACTCAGAAGGAAGCCGTTCCTCAACTCCCTTTAAACCAACTGCAGCCAAGGTTTCCTTGACAAGCTCTGATATTTGTTCCTCAGGCATGCTTGAGTGTTCATACAAGAAGAAAACCAACATTTTCACGAACAGTCAAAGAATAAATAGTGCCGCGCTTTGGAACACTAAGCCAATCCGAAGACCTGATATCTCGTCATCACTTATCAAACCACTTCTCTTTCTACCTCGAATATAAACTTCTCCCTTGTCCGGAGCAAGAAGTCCCGCAATAATCTTCAAAATTGTAGATTTGCCAGTCCCAGAGGGACCAATTATTCCAACAGCTTCACCATATCTGATCTTGAAGCTGATGCCATTTAAGATTTTCTTTTCCCCAAAAGACTTGTAGACGTCTCTACACTCAATAAGAACATCAGAATCATCGTCATGATCCCGAATTGTGTTTAATTGTTCTGATTTGGATGAACCATTGAATTGAATGGCGGAAGAGTCTTGGGTGTTGAAATTACGTGGAGGAGCGATGCAAGCACAAACAACTTTTCTGCGAATTTTGTTAGAGTGGGGATTGGCGTTGTTGTTGTTTGTTTGTTGAAATCTAGTAGAAGGGGGGTATTTTTGTGTGGAGTGAGGCGAAAGAAAGGGTTGGTTGAGAGGGAAACCATGGTTAGGGGATTTGGATAATATAGATGGATGGAGAAGTTATTGGGTTATAGACATGGAAAGTAGCATGTGATTCATGTGGAGAACGAAGAGTGGAGTTTTCAAAAGGGTGGTGATGATGAAGAAGATGAGTGAGTCAAATGGGGATGATTGGTCGTCGGTGATTTTGAGAAGTCGCTTTCCCGGAAGATGGCTATTGGACTTGCCTGAGAGGGCCTCACTCCATCGACTTTCTCTTTGTTTATTATTTAACTCAAAGNAAAATTCTCCTTTGGAGTAAATATCAATATGTCCTCATACCTAATT
TARGET=691,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1140
PRIMER_LEFT_PENALTY=0.101896
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TCTTGGGTGTTGAAATTACGTG
PRIMER_RIGHT_SEQUENCE=CACCCTTTTGAAAACTCCACTC
PRIMER_LEFT=610,22
PRIMER_RIGHT=891,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.3174
PRIMER_LEFT_1_PENALTY=0.101896
PRIMER_RIGHT_1_PENALTY=0.215550
PRIMER_LEFT_1_SEQUENCE=TCTTGGGTGTTGAAATTACGTG
PRIMER_RIGHT_1_SEQUENCE=AACTCCACTCTTCGTTCTCCAC
PRIMER_LEFT_1=610,22
PRIMER_RIGHT_1=879,22
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=59.784
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.3564
PRIMER_LEFT_2_PENALTY=0.101896
PRIMER_RIGHT_2_PENALTY=0.254467
PRIMER_LEFT_2_SEQUENCE=TCTTGGGTGTTGAAATTACGTG
PRIMER_RIGHT_2_SEQUENCE=CATCACCACCCTTTTGAAAACT
PRIMER_LEFT_2=610,22
PRIMER_RIGHT_2=897,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.254
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.3829
PRIMER_LEFT_3_PENALTY=0.101896
PRIMER_RIGHT_3_PENALTY=0.281044
PRIMER_LEFT_3_SEQUENCE=TCTTGGGTGTTGAAATTACGTG
PRIMER_RIGHT_3_SEQUENCE=AAACTCCACTCTTCGTTCTCCA
PRIMER_LEFT_3=610,22
PRIMER_RIGHT_3=880,22
PRIMER_LEFT_3_TM=59.898
PRIMER_RIGHT_3_TM=60.281
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=271
PRIMER_PAIR_PENALTY_4=0.3925
PRIMER_LEFT_4_PENALTY=0.101896
PRIMER_RIGHT_4_PENALTY=0.290589
PRIMER_LEFT_4_SEQUENCE=TCTTGGGTGTTGAAATTACGTG
PRIMER_RIGHT_4_SEQUENCE=CACTCTTCGTTCTCCACATGAA
PRIMER_LEFT_4=610,22
PRIMER_RIGHT_4=874,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=60.291
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_04295 Ps_contig_mira-and-tgicl-ass_franssen_32780
SEQUENCE=ATCAAGTCCAGCAGTCGGTTCGTCATTTACTAAGAGAACCTCTGGTTCTAATGAATCCTTTGTGGTGTCACAAATAATAGATCGAGCTAAAGCAACTCGCTTTTTCATTCCGCCTGATAACTCAGAAGGAAGCCGTTCCTCAACTCCCTTTAAACCAACTGCAGCCAAGGTTTCCTTGACAAGCTCTGATATTTGTTCCTCAGGCATGCTTGAGTGTTCATACAAGAAGAAAACCAACATTTTCACGAACAGTCAAAGAATAAATAGTGCCGCGCTTTGGAACACTAAGCCAATCCGAAGACCTGATATCTCGTCATCACTTATCAAACCACTTCTCTTTCTACCTCGAATATAAACTTCTCCCTTGTCCGGAGCAAGAAGTCCCGCAATAATCTTCAAAATTGTAGATTTGCCAGTCCCAGAGGGACCAATTATTCCAACAGCTTCACCATATCTGATCTTGAAGCTGATGCCATTTAAGATTTTCTTTTCCCCAAAAGACTTGTAGACGTCTCTACACTCAATAAGAACATCAGAATCATCGTCATGATCCCGAATTGTGTTTAATTGTTCTGATTTGGATGAACCATTGAATTGAATGGCGGAAGAGTCTTGGGTGTTGAAATTACGTGGAGGAGCGATGCAAGCACAAACAACTTTTCTGCGAATTTTGTTAGAGTGGGGATTGGCGTTGTTGTTGTTTGTTTGTTGAAATCTAGTAGAAGGGGGGTATTTTTGTGTGGAGTGAGGCGAAAGAAAGGGTTGGTTGAGAGGGAAACCATGGTTAGGGGATTTGGATAATATAGATGGATGGAGAAGTTATTGGGTTATAGACATGGAAAGTAGCATGTGATTCATGTGGAGAACGAAGAGTGGAGTTTTCAAAAGGGTGGTGATGATGAAGAAGATGAGTGAGTCAAATGGGGATGATTGGTCGTCGGTGATTTTGAGAAGTCGCTTTCCCGGAAGATGGCTATTGGACTTGCCTGAGAGGGCCTCACTCCATCGACTTTCTCTTTGTTTATTATTTAACTCAAAGNAAAATTCTCCTTTGGAGTAAATATCAATATGTCCTCATACCTAATT
TARGET=698,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1140
PRIMER_LEFT_PENALTY=0.101896
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TCTTGGGTGTTGAAATTACGTG
PRIMER_RIGHT_SEQUENCE=CACCCTTTTGAAAACTCCACTC
PRIMER_LEFT=610,22
PRIMER_RIGHT=891,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.3174
PRIMER_LEFT_1_PENALTY=0.101896
PRIMER_RIGHT_1_PENALTY=0.215550
PRIMER_LEFT_1_SEQUENCE=TCTTGGGTGTTGAAATTACGTG
PRIMER_RIGHT_1_SEQUENCE=AACTCCACTCTTCGTTCTCCAC
PRIMER_LEFT_1=610,22
PRIMER_RIGHT_1=879,22
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=59.784
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.3564
PRIMER_LEFT_2_PENALTY=0.101896
PRIMER_RIGHT_2_PENALTY=0.254467
PRIMER_LEFT_2_SEQUENCE=TCTTGGGTGTTGAAATTACGTG
PRIMER_RIGHT_2_SEQUENCE=CATCACCACCCTTTTGAAAACT
PRIMER_LEFT_2=610,22
PRIMER_RIGHT_2=897,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.254
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.3829
PRIMER_LEFT_3_PENALTY=0.101896
PRIMER_RIGHT_3_PENALTY=0.281044
PRIMER_LEFT_3_SEQUENCE=TCTTGGGTGTTGAAATTACGTG
PRIMER_RIGHT_3_SEQUENCE=AAACTCCACTCTTCGTTCTCCA
PRIMER_LEFT_3=610,22
PRIMER_RIGHT_3=880,22
PRIMER_LEFT_3_TM=59.898
PRIMER_RIGHT_3_TM=60.281
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=271
PRIMER_PAIR_PENALTY_4=0.3925
PRIMER_LEFT_4_PENALTY=0.101896
PRIMER_RIGHT_4_PENALTY=0.290589
PRIMER_LEFT_4_SEQUENCE=TCTTGGGTGTTGAAATTACGTG
PRIMER_RIGHT_4_SEQUENCE=CACTCTTCGTTCTCCACATGAA
PRIMER_LEFT_4=610,22
PRIMER_RIGHT_4=874,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=60.291
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_04296 Ps_contig_mira-and-tgicl-ass_franssen_32783
SEQUENCE=GTCAAAGCTTGATTTATTGAGAAAGGCAGAGCAGCATCTATAAGCAGTCAGACTTCCAGAAACTACAAAATTTCTTACAACAACAATACCAATTCACTATAAAATACATCTATATGTGCTTTTGCAGGATGGCACTCTAGAAACAATGTTGACAAAAAAGACAAAGAGAAAAAACATAGTTTAACCTTTATATATTAATACAAAGCACGCACCCAAAAACATACATATTTGCCAGAATTTGAGATGCAGACCAAGAGCCGGATCCTCTTGAGGAAAATTTGAACTTTTCATCAAATCAAAGTGACAATCAAGTAAACACTAGCCAAAATGAAGATTTCATCTGAGGCCAACTACTGATTTATGAATCCGCTTCATTTTCAGAAGGGCACTTCTTCTGTTTCTAATTTGGCTGACAGAGATTCTTGAAAATCAAGGTTCACCGGAAATAGATTACTTTGTGCATATTGGGCCATCAATTGATCCACAAGCACCAAAGCTACCATAGCCTCTACCATGGGTACAGCTCTTGGGACAACACAAGGATCATGACGACCACGGGCTATGAGATCTGTTTCTTTCTTATCCCGAGTCACTGTAGATTGCTTCTTTGAAATAGTTGATGTCGGCTTGAAAGCTACTCTCATGTTAATTATTTCTCCATTGGAAATCCCACCCTGTATCCCACCAGAGCGGTTTGTTCTTGTCCTCACATTTCCATTTTGATCTATATAAAATTCATCATTGTGTTCACTTCCAGTCAAAAACGTACCTGCAAACCCACTACCAAATTGAAAACCCTTAGTTGCAGGCAATGACATAGCAGCTTTGGCCAGCTCAGCTTCAAGTTTGTCAAATACCGGCGAACCAAGACCGCGTGGACAGTTCCTCACAATGCATGTTACAACACCACCAACAGAATCACCTTTCACTCGGACAGCATCAATAGCAGCTATCATCTTCTCTGCATATTCAGGGTCTGGACATCGAACAATGTTACTCTCAATCTGATCAAGTGTCAATGTGTCGTTGTCAATTAAGTCCTCGGGAAGAATAATCTTGTGAACTTGAGAGACATAGGCAAGAATCTCAGTTCCAGCAAACTTTTTAAGGATTTTCTTAGCAACAGCACCAGAAGCAACCCTTCCAATGGTTTCTCTAGCAGAAGATCTACCACCACCCTGAACTGATCTGACACCATACTTCATGTCATAGGTTGCATCTGCATGGGAAGGCCTATAAGCTAATGACATCTCGCTATAGTCATTTCCTCTTTGATCAGTATTGGGCACAAATACATGGATTGGAGTCCCTGTCGTAACTCCATCCGAAACTCCTGAAAATATTTTGCATGTATCAGTCTCCTTTCTAGGAGTTGTAATTCGGCTCTGACCAGGCCTCCTTCTGTCAAGATCCTCTTGCATATCGGCTTCAGAGAGAGGAATGCGAGGAGGACATCCATCAATAACACAACCAACACCTCCTCCATGAGATTCTCCATATGTCGTAACGCGGAAGTGATTCCCATAGGTACTCCCAGCTGCCCGAATCTGGAGTTTCTTTGGGAAGCGGGGACGGAGGTAAACGGTGGAGAGAGATCGGAGATCGGAATTAGGAGAAACGAATGCGGTGAAGGAATCGGTTCTGGGAACGGTGGAGGGTAAGGGTTTGGNGAAGAGAGAAGAAGCCATGAGTGGGTATTGGGAATAGGAAAAGAGAATGATAGAAATTCTTTGTTTCTTTCGTAGGGTTTGGGTTGGTTTGTTGTTGTTGTT
TARGET=1759,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04297 Ps_contig_mira-and-tgicl-ass_franssen_32791
SEQUENCE=CAACAATATTGCACAATATCATATTCTATGCAATGTTACAATTAAAATATTACCTRTTTAATATCTTTATTTGCTAAGAAAAATTAATTAATTGAAAAACTAGTTATTGTTGGTATTGATATTTTGTAGTGAAAGCTTAATTTGAAGCTGAGACATAAGGTCTTGATTCTCTTTATGCAAAAGCATAGCTTGTTTCCTCAACTTTTCATTCTCCTCAATGATGGTTTGATTCTCCATATACAATTTCAAGTTCTTCATCTTAATCTCTTCTTTCACTATAATCATCTTTTTCCTTTCTTTTGCTTCTTTCAATTGTGTTCTCCTTCTTTTGAGGTGAGTTAATCTAAGGTTCCTATGTTTCTTGGAGAGAGGAGCATAAAAGGTGTCATGTTGTGAAGAGAAACAACACATTATGTTTGGTTTTTTGAAATGAAAAATGAGAAGGTTAAGAAGAGATATTGAAAGATGGTTAGTGAAGGTTAAGGGGTTTATAT
TARGET=82,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04298 Ps_contig_mira-and-tgicl-ass_franssen_32797
SEQUENCE=GGTTTAATTAAGCAATGAACNAAAATTAGAATAACAGCTAACAANTAAGCTTAAAATATTCATCAACATTATTCAAATACTCATTACATTCAAAACGCAACACTGATACTAAACTTATACTCTAGAATTATAAAAACAAACAAACATTACATATAACATCTGAACAAAGTTCAGTTATTCTCAAAACTCAAAAGACATCCCTTATAATCCTCAAATGGATCTTTTCTTAATATTAACTTTCAAGCCATCTTTCACATACAAAACAACAGAAACACTTGGGATTATAGGATGATCTTCCACCACATGAACATGATAATTCAACAACATTGCTACAGCAACCATTTTCAACTGAATGAAACTTATATCCTTGCCCAAACAGCTCCTTGGTCCAGCATTGAAAGCAATGAACTTGTAAGAAGGTACATGAATTGTCCCTCCTCTTTTAGAAATCCACCTCTCTGGCTTAAATTCCAAGCAATCTTCTCCCCATATTTCTTCCACCCTTCCAACTGAGTATAGAGAATACACTATCATTGTATTTTCCTTAACCTTATGTCCACTAGGAAGCACATCATGTTCCAATGGAGATTTATGCTCAAAAGGAACCGGTGGATAAAGCCTTAAGGCTTCTAATAATGCTCCATGAAGATAAACTAACTTGCTAAGCCCTTCCACTCCTAAATCTTTCTTGTTATCTTCTTTTGATGCTAGTTTTTCTTTGATCTCTTCAAGAATCTTAGCTTCAACAAAAGGGTGTGTTGCAACCAACCAGAAAAACCAAGTCAGACTAGAACTGATAGTGTCTCTTCCAGCTGCAAGAAGATTTATTGCTGTGTCTCTCAGAAACTTGTCATCTATTCGGTTTTCTCCATCTCTCACTTCATTCATAAGGGTACTTAGCAAGTCGGGTTTTTGTTGGTTTTGATTTTTAATATCTCTTTTGGATTTGATTTCTGAATATAACATTTCATCGACGATTTTTTGATTCTCTATAAACGTTTTCTCTTCACCAACTCGAAGCCATTTCTGCAACTTCCACAAGAATCTTGGTCTTATATGTCTATAGAACAATGTGTCTTCTGCATGAGTGAAAGCTCTCTCACATGCAATTTCAGGGAGATCATTAGAGAGACACATAGGATCAAACCCTAAAACTACCGAACATATATTATCAAAAGTTAACCTTTGAAACACATCTTGCAAATCCACTTGCATCCCATTTTTACAAGCATGATCAAGAAACACAAGAAGATGACTGTAAATTTTTTTCTCAGTGGTTTTTTGAATAAATACCTGAAAGCTTTCCTGCCTGAAAATTGAATGAAGTAGGGTTCTGTTGTACTTCCAAATGTCAGAGTCAGACCTGAAAATTCCATCTCCAAGAACTTCAAAGATCTCTCTGAAATCATTTCCTTTCCCATAGTTTTCAAACTTTGTGCTTGTAATGTGCTGCACATTCATGGGATCACAAGTGAAAACAATGTTCATGTTTGCTAGCCACGGTCCTTCGAAAGTGAAAGTTCCTCCACATTGTTTTAGTTTTGAGTTTAAATAATCATGGATATTGGATGTGTTGTAAAAGAGACCAGGTAGCATTCCCACCACAGGCCACTTTGTTATCGGTGTGTTTCTGTTTAGCTTCCAATAATGGATTGCAGTGAAAAGAATAATCGCCAAAAGAATTTCAACAACGCCAAACAAAGCCATTATTCTGTTGGCCTAGATATAGTTAAGCATGAATATTGGCAATGGTGTGTCCCGACT
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.6941
PRIMER_LEFT_PENALTY=3.683988
PRIMER_RIGHT_PENALTY=0.010077
PRIMER_LEFT_SEQUENCE=CATTCAAAACGCAACACTGA
PRIMER_RIGHT_SEQUENCE=GCTGTTTGGGCAAGGATATAAG
PRIMER_LEFT=86,20
PRIMER_RIGHT=379,22
PRIMER_LEFT_TM=58.316
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=3.6941
PRIMER_LEFT_1_PENALTY=3.683988
PRIMER_RIGHT_1_PENALTY=0.010077
PRIMER_LEFT_1_SEQUENCE=CATTCAAAACGCAACACTGA
PRIMER_RIGHT_1_SEQUENCE=AGCTGTTTGGGCAAGGATATAA
PRIMER_LEFT_1=86,20
PRIMER_RIGHT_1=380,22
PRIMER_LEFT_1_TM=58.316
PRIMER_RIGHT_1_TM=59.990
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=3.9245
PRIMER_LEFT_2_PENALTY=3.683988
PRIMER_RIGHT_2_PENALTY=0.240472
PRIMER_LEFT_2_SEQUENCE=CATTCAAAACGCAACACTGA
PRIMER_RIGHT_2_SEQUENCE=ATCATGTTCATGTGGTGGAAGA
PRIMER_LEFT_2=86,20
PRIMER_RIGHT_2=313,22
PRIMER_LEFT_2_TM=58.316
PRIMER_RIGHT_2_TM=60.240
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=228
PRIMER_PAIR_PENALTY_3=3.9245
PRIMER_LEFT_3_PENALTY=3.683988
PRIMER_RIGHT_3_PENALTY=0.240472
PRIMER_LEFT_3_SEQUENCE=CATTCAAAACGCAACACTGA
PRIMER_RIGHT_3_SEQUENCE=ATGTTCATGTGGTGGAAGATCA
PRIMER_LEFT_3=86,20
PRIMER_RIGHT_3=310,22
PRIMER_LEFT_3_TM=58.316
PRIMER_RIGHT_3_TM=60.240
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=3.9245
PRIMER_LEFT_4_PENALTY=3.683988
PRIMER_RIGHT_4_PENALTY=0.240472
PRIMER_LEFT_4_SEQUENCE=CATTCAAAACGCAACACTGA
PRIMER_RIGHT_4_SEQUENCE=TCATGTTCATGTGGTGGAAGAT
PRIMER_LEFT_4=86,20
PRIMER_RIGHT_4=312,22
PRIMER_LEFT_4_TM=58.316
PRIMER_RIGHT_4_TM=60.240
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_04299 Ps_contig_mira-and-tgicl-ass_franssen_32798
SEQUENCE=CAAAAGAAAGGGAAAGAAAAGAAGCTGCCAGAAAAAAGAAAAGCAAATCAAAAGAAGAAGCTAACACCACTCGTTCATCTTCCATAGAAAGTGAAATTATTTGGTGTTTTGTGAATCAATCTTTCATCAAAGTTTTGTTCTTTAGTTATCCTCCTCCTCCTCCACATGGGTCGCCTTATTTTCTTTCTCATCATTCTAACCTTCTTTACATTATCTTTCCTCTCAGGTAAACTTTCACAAACTCTCTATTTTTATTTTCAAAAATTGCACTTTAGTCTCTAAATTTTAACTATTTTCCATTTTGGTCCTTGAACTCTTTTCAGAGGTACAGTCAACATCGTTCAAGATTGTAAACAAGTGCCGTCACACAATATGGCCAGGATTACTCTCAGGCGCCACCTCGCCGCCGCTACCCACTACCGGATTCACCCTCAAGAGCGGAGAGTCAAGGACTGTCAACATCCCGAAATCTTGGTCGGGTCGGTTATGGGCACGGACTCTCTGCGGCCGTGGCTCCGACGGGAAATTCTCGTGTGTCACCGCTGACTGTGGCTCTGGTAAAGTAGAGTGCGCTGGCGGTGGGGCTAAGCCGCCGGCATCTTTGGCGGAGTTCACCTTGAACGGAGCCGACGGGTTGGATTTCTACGACGTAAGTTTGGTCGACGGTTATAATCTTCCGATGCTGGTTGTGGCTAAAGGCGGGACTAAGGGAGGATGCAGCCCTACTGGATGTCTTGTTGATCTTAACGGAGGTTGCCCGGCGGATCTTAAGGTGGCGCGTGGTGATGGAAGTGGCGATAACGTTGCGTGTAGAAGCGCGTGTGAAGCTTTTGCGGATCCTCGTTATTGTTGTAGTGAGGCGTACTCTACGCCTGACACGTGTGCTCCTTCTGTATACTCGCAGTTTTTTAAGCATGCATGCCCACGCTCATATAGCTACGCGTATGATGATAAGACTAGCACATTCACGTGTGCCTCCGCTAACTATATGATCATATTTTGCCCCTTACCTTATACTAGGTACCAAAAAGTGTTGGGAGCACGGCAAGACGGGGCGC
TARGET=149,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1725
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=0.165541
PRIMER_LEFT_SEQUENCE=CACCACTCGTTCATCTTCCATA
PRIMER_RIGHT_SEQUENCE=CGGCACTTGTTTACAATCTTGA
PRIMER_LEFT=64,22
PRIMER_RIGHT=362,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=60.166
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=0.2700
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=0.263023
PRIMER_LEFT_1_SEQUENCE=CACCACTCGTTCATCTTCCATA
PRIMER_RIGHT_1_SEQUENCE=TGAGAAAGAAAATAAGGCGACC
PRIMER_LEFT_1=64,22
PRIMER_RIGHT_1=189,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=59.737
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.3921
PRIMER_LEFT_2_PENALTY=0.129103
PRIMER_RIGHT_2_PENALTY=0.263023
PRIMER_LEFT_2_SEQUENCE=GAAAGAAAAGAAGCTGCCAGAA
PRIMER_RIGHT_2_SEQUENCE=TGAGAAAGAAAATAAGGCGACC
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=189,22
PRIMER_LEFT_2_TM=60.129
PRIMER_RIGHT_2_TM=59.737
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.4314
PRIMER_LEFT_3_PENALTY=0.006945
PRIMER_RIGHT_3_PENALTY=0.424445
PRIMER_LEFT_3_SEQUENCE=CACCACTCGTTCATCTTCCATA
PRIMER_RIGHT_3_SEQUENCE=GAGAAAGAAAATAAGGCGACCC
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=188,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=60.424
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=0.5075
PRIMER_LEFT_4_PENALTY=0.006945
PRIMER_RIGHT_4_PENALTY=0.500551
PRIMER_LEFT_4_SEQUENCE=CACCACTCGTTCATCTTCCATA
PRIMER_RIGHT_4_SEQUENCE=AAGAGTTCAAGGACCAAAATGG
PRIMER_LEFT_4=64,22
PRIMER_RIGHT_4=317,22
PRIMER_LEFT_4_TM=59.993
PRIMER_RIGHT_4_TM=59.499
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_04300 Ps_contig_mira-and-tgicl-ass_franssen_32801
SEQUENCE=AAGCAGNGGTAGTCAACGCAGAGTANCGGCGTAGGGAATATAATTGTGCCGGAGGAGCACTCCTGGATCCNGATCCAAGCGTGAGCAAGTCCAATAAAAACAAAGTAAACATAATTACTGAGAAAGCAGACTCGGTTGCTCCGCCGCGCTGAGACAATAATGACTGTACTCCGCTTGATTCTGGCGGTTACTTTATTTCTTACCTGCTTCATCTCTCTCTCACCAGCTTTCCAATCCGACGAGCTTCTCCTCGACGACGAAGAATTCGGTCTCGAAGGTGGACGCCCTCAATCCCGTCCATCCCCTAACACCGCCACCACCACCACCACCACTATCCCCACTCGCAAGAGGATTCCAGACTCCGCTTCCGATTCCAAGATCCAGTTCACACTTGAACACGCTTTCGGAGATTCTGATTTCTACGAAGCCGGTAACTTCTCCGCTCGTCTCAAAACATGGAGTCACGGTGCACAGACACTGACGAAGCTGCGGTTTTCGCGAGATTCTTTCACTGATGATGAGAAGAATAAATTTCAAGAGCTGTTGAAAGGAGATGATTTCTATAGGATTAGACTGCCATCCAATGTTTTAAACCCTCCAGGCAGGGAATATATTGTTTCTTCAGTGAAAGCTAGATGTCTTACAGGGGATGGTTTGGAGGAACACTTTGTAATAAACACGGAAGGTGTTAACATCTTGGCTGTCAATTATGGTGCTCCTGGGGCTTGCCCTTATCCCCGGCAACTGAAGCTTCCTGCAAAGTGGTCTTTCAAGTCACACACGCTTTTGAAGAACACTGAACAGGCACCAAGGACACCAATATTTACAGAAGAGGTATTTGGTGGAGAGGGGATAGAAGGTGAAATTGTACAGCCAATAGAAAGATCCTTTTGGGCTAAATACTGGATGTATTTGATCCCTCTCGGACTCATTGTAATGAACGCCATTACCCAAGCAGCGAATATGCCTGAGGAACAGGCTGGTGGTCAAGCTGGGGCACCACCACAGCCAGGAACTGCAGTACAGAGGGGAACAAATTCTGGTGTGCGTAGAAGATGATAATTTTGGTTTCATCTACCTAAGAAAACATTATTTAGGGACACTGATCATGAATGAAATTTTAGATTTTCCTTTTATGTAACATTTTAAAATAATTTAGTTACAGGTATCAGTTCTGTATTGGGGAACATTGAAACAGCAGGGATAAAATTTTCCAGTTAGATTGAGTAATTAATTTTAAATTTTAACACGAAAATCCAGTCCGGTATCCTCTGGTTTTTGTGATGATTCTGTGGTTATGTTATATGATGCGAAGAAATATTAAATAGTTTTTGAATGGTATGAAGTTTATTAGGTTTTGAC
TARGET=212,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7538
PRIMER_LEFT_PENALTY=0.680486
PRIMER_RIGHT_PENALTY=0.073355
PRIMER_LEFT_SEQUENCE=ACTGTACTCCGCTTGATTCTGG
PRIMER_RIGHT_SEQUENCE=CGTGTTCAAGTGTGAACTGGAT
PRIMER_LEFT=162,22
PRIMER_RIGHT=399,22
PRIMER_LEFT_TM=60.680
PRIMER_RIGHT_TM=60.073
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.7675
PRIMER_LEFT_1_PENALTY=0.680486
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=ACTGTACTCCGCTTGATTCTGG
PRIMER_RIGHT_1_SEQUENCE=GAGAAGTTACCGGCTTCGTAGA
PRIMER_LEFT_1=162,22
PRIMER_RIGHT_1=439,22
PRIMER_LEFT_1_TM=60.680
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.8290
PRIMER_LEFT_2_PENALTY=0.680486
PRIMER_RIGHT_2_PENALTY=0.148509
PRIMER_LEFT_2_SEQUENCE=ACTGTACTCCGCTTGATTCTGG
PRIMER_RIGHT_2_SEQUENCE=CAAGTGTGAACTGGATCTTGGA
PRIMER_LEFT_2=162,22
PRIMER_RIGHT_2=393,22
PRIMER_LEFT_2_TM=60.680
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.8290
PRIMER_LEFT_3_PENALTY=0.680486
PRIMER_RIGHT_3_PENALTY=0.148509
PRIMER_LEFT_3_SEQUENCE=ACTGTACTCCGCTTGATTCTGG
PRIMER_RIGHT_3_SEQUENCE=TCAAGTGTGAACTGGATCTTGG
PRIMER_LEFT_3=162,22
PRIMER_RIGHT_3=394,22
PRIMER_LEFT_3_TM=60.680
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.8873
PRIMER_LEFT_4_PENALTY=0.680486
PRIMER_RIGHT_4_PENALTY=0.206859
PRIMER_LEFT_4_SEQUENCE=ACTGTACTCCGCTTGATTCTGG
PRIMER_RIGHT_4_SEQUENCE=GAAGTTACCGGCTTCGTAGAAA
PRIMER_LEFT_4=162,22
PRIMER_RIGHT_4=437,22
PRIMER_LEFT_4_TM=60.680
PRIMER_RIGHT_4_TM=59.793
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_04301 Ps_contig_mira-and-tgicl-ass_franssen_32801
SEQUENCE=AAGCAGNGGTAGTCAACGCAGAGTANCGGCGTAGGGAATATAATTGTGCCGGAGGAGCACTCCTGGATCCNGATCCAAGCGTGAGCAAGTCCAATAAAAACAAAGTAAACATAATTACTGAGAAAGCAGACTCGGTTGCTCCGCCGCGCTGAGACAATAATGACTGTACTCCGCTTGATTCTGGCGGTTACTTTATTTCTTACCTGCTTCATCTCTCTCTCACCAGCTTTCCAATCCGACGAGCTTCTCCTCGACGACGAAGAATTCGGTCTCGAAGGTGGACGCCCTCAATCCCGTCCATCCCCTAACACCGCCACCACCACCACCACCACTATCCCCACTCGCAAGAGGATTCCAGACTCCGCTTCCGATTCCAAGATCCAGTTCACACTTGAACACGCTTTCGGAGATTCTGATTTCTACGAAGCCGGTAACTTCTCCGCTCGTCTCAAAACATGGAGTCACGGTGCACAGACACTGACGAAGCTGCGGTTTTCGCGAGATTCTTTCACTGATGATGAGAAGAATAAATTTCAAGAGCTGTTGAAAGGAGATGATTTCTATAGGATTAGACTGCCATCCAATGTTTTAAACCCTCCAGGCAGGGAATATATTGTTTCTTCAGTGAAAGCTAGATGTCTTACAGGGGATGGTTTGGAGGAACACTTTGTAATAAACACGGAAGGTGTTAACATCTTGGCTGTCAATTATGGTGCTCCTGGGGCTTGCCCTTATCCCCGGCAACTGAAGCTTCCTGCAAAGTGGTCTTTCAAGTCACACACGCTTTTGAAGAACACTGAACAGGCACCAAGGACACCAATATTTACAGAAGAGGTATTTGGTGGAGAGGGGATAGAAGGTGAAATTGTACAGCCAATAGAAAGATCCTTTTGGGCTAAATACTGGATGTATTTGATCCCTCTCGGACTCATTGTAATGAACGCCATTACCCAAGCAGCGAATATGCCTGAGGAACAGGCTGGTGGTCAAGCTGGGGCACCACCACAGCCAGGAACTGCAGTACAGAGGGGAACAAATTCTGGTGTGCGTAGAAGATGATAATTTTGGTTTCATCTACCTAAGAAAACATTATTTAGGGACACTGATCATGAATGAAATTTTAGATTTTCCTTTTATGTAACATTTTAAAATAATTTAGTTACAGGTATCAGTTCTGTATTGGGGAACATTGAAACAGCAGGGATAAAATTTTCCAGTTAGATTGAGTAATTAATTTTAAATTTTAACACGAAAATCCAGTCCGGTATCCTCTGGTTTTTGTGATGATTCTGTGGTTATGTTATATGATGCGAAGAAATATTAAATAGTTTTTGAATGGTATGAAGTTTATTAGGTTTTGAC
TARGET=314,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3284
PRIMER_LEFT_PENALTY=0.254998
PRIMER_RIGHT_PENALTY=0.073355
PRIMER_LEFT_SEQUENCE=CTGCTTCATCTCTCTCTCACCA
PRIMER_RIGHT_SEQUENCE=CGTGTTCAAGTGTGAACTGGAT
PRIMER_LEFT=203,22
PRIMER_RIGHT=399,22
PRIMER_LEFT_TM=59.745
PRIMER_RIGHT_TM=60.073
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=0.3284
PRIMER_LEFT_1_PENALTY=0.254998
PRIMER_RIGHT_1_PENALTY=0.073355
PRIMER_LEFT_1_SEQUENCE=TGCTTCATCTCTCTCTCACCAG
PRIMER_RIGHT_1_SEQUENCE=CGTGTTCAAGTGTGAACTGGAT
PRIMER_LEFT_1=204,22
PRIMER_RIGHT_1=399,22
PRIMER_LEFT_1_TM=59.745
PRIMER_RIGHT_1_TM=60.073
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=0.3420
PRIMER_LEFT_2_PENALTY=0.254998
PRIMER_RIGHT_2_PENALTY=0.087031
PRIMER_LEFT_2_SEQUENCE=TGCTTCATCTCTCTCTCACCAG
PRIMER_RIGHT_2_SEQUENCE=GAGAAGTTACCGGCTTCGTAGA
PRIMER_LEFT_2=204,22
PRIMER_RIGHT_2=439,22
PRIMER_LEFT_2_TM=59.745
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.3420
PRIMER_LEFT_3_PENALTY=0.254998
PRIMER_RIGHT_3_PENALTY=0.087031
PRIMER_LEFT_3_SEQUENCE=CTGCTTCATCTCTCTCTCACCA
PRIMER_RIGHT_3_SEQUENCE=GAGAAGTTACCGGCTTCGTAGA
PRIMER_LEFT_3=203,22
PRIMER_RIGHT_3=439,22
PRIMER_LEFT_3_TM=59.745
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=0.4035
PRIMER_LEFT_4_PENALTY=0.254998
PRIMER_RIGHT_4_PENALTY=0.148509
PRIMER_LEFT_4_SEQUENCE=CTGCTTCATCTCTCTCTCACCA
PRIMER_RIGHT_4_SEQUENCE=CAAGTGTGAACTGGATCTTGGA
PRIMER_LEFT_4=203,22
PRIMER_RIGHT_4=393,22
PRIMER_LEFT_4_TM=59.745
PRIMER_RIGHT_4_TM=60.149
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_04302 Ps_contig_mira-and-tgicl-ass_franssen_32810
SEQUENCE=GTCGGGTTTCAAGCTATAGCCGCATCAGAACCAATCGAAGCCAGAAGGAAAAATCAAAATTCTTCATAATGGGTAAGGTTCATGGATCTTTGGCCCGTGCCGGGAAAGTGAGAGGGCAAACTCCTAAGGTTGCGAAGCAAGACAAGAAGAAGAAGCCACGTGGTCGTGCTCACAAGCGTATGCAATACAATCGCCGATTCGTCACCGCCGTTGTGGGATTCGGTAAGAAGCGTGGACCTAACTCATCTGAGAAGTGAGGGTGATGCATAGTGGAAGGTTTTTTAATTAGATTGCAGAATGTACTTTTTATTTTTATTTTTAAACGATGAAAGAGTTTGATTGAGGAACTATTGAATTTCTGACTATCTTTAAATGAAAGAGTTCTTTTTGTTCTAGAAAACTTCATAATTATGCAAGGTTCATTAGTTGTTGT
TARGET=144,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2063
PRIMER_LEFT_PENALTY=0.158697
PRIMER_RIGHT_PENALTY=0.047559
PRIMER_LEFT_SEQUENCE=GTAAGGTTCATGGATCTTTGGC
PRIMER_RIGHT_SEQUENCE=CGATTGTATTGCATACGCTTGT
PRIMER_LEFT=72,22
PRIMER_RIGHT=192,22
PRIMER_LEFT_TM=59.841
PRIMER_RIGHT_TM=60.048
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.3437
PRIMER_LEFT_1_PENALTY=0.296177
PRIMER_RIGHT_1_PENALTY=0.047559
PRIMER_LEFT_1_SEQUENCE=GGTAAGGTTCATGGATCTTTGG
PRIMER_RIGHT_1_SEQUENCE=CGATTGTATTGCATACGCTTGT
PRIMER_LEFT_1=71,22
PRIMER_RIGHT_1=192,22
PRIMER_LEFT_1_TM=59.704
PRIMER_RIGHT_1_TM=60.048
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=0.3798
PRIMER_LEFT_2_PENALTY=0.158697
PRIMER_RIGHT_2_PENALTY=0.221115
PRIMER_LEFT_2_SEQUENCE=GTAAGGTTCATGGATCTTTGGC
PRIMER_RIGHT_2_SEQUENCE=GATGAGTTAGGTCCACGCTTCT
PRIMER_LEFT_2=72,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=59.841
PRIMER_RIGHT_2_TM=59.779
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=175
PRIMER_PAIR_PENALTY_3=0.3798
PRIMER_LEFT_3_PENALTY=0.158697
PRIMER_RIGHT_3_PENALTY=0.221115
PRIMER_LEFT_3_SEQUENCE=GTAAGGTTCATGGATCTTTGGC
PRIMER_RIGHT_3_SEQUENCE=AGATGAGTTAGGTCCACGCTTC
PRIMER_LEFT_3=72,22
PRIMER_RIGHT_3=247,22
PRIMER_LEFT_3_TM=59.841
PRIMER_RIGHT_3_TM=59.779
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=0.4078
PRIMER_LEFT_4_PENALTY=0.158697
PRIMER_RIGHT_4_PENALTY=0.249074
PRIMER_LEFT_4_SEQUENCE=GTAAGGTTCATGGATCTTTGGC
PRIMER_RIGHT_4_SEQUENCE=AAACCTTCCACTATGCATCACC
PRIMER_LEFT_4=72,22
PRIMER_RIGHT_4=279,22
PRIMER_LEFT_4_TM=59.841
PRIMER_RIGHT_4_TM=60.249
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_04303 Ps_contig_mira-and-tgicl-ass_franssen_32814
SEQUENCE=GGTGTAAGAATAACAAAACTAGTATGGCTACTACTCATTTTCGCCGTGTTTTTGTACACTAAGGTGAATATCAAATGCCAAGTTTTGACAATGCTCGTCGGCAGTATCAAGTGACTTGCATTGCAAGGAACGGCATTCGTGATCCTCAGCGAGAGGAACTGACACCAACTGGGGTGCTTTTACTATTTTGTGTTCTACCGTGCCGAAGCTCATTCCATCTCCGAATGAGAAAACAGTCTGTCCTTTATAAAATCTATAAGTTCTTCCAGGGTAACCGGTAGCAGGATCCGCCCTCATGTTCATGTTCGTCATTGGTACCTTTTCTACATATGATTGCGGATACCATGTCATCGGTAGCCTTCCACTTGGATTGTACGAACCGAAAATCACATCGGCTATGGCAGCTCCACCGGCTTCGCCAGGGTAGCCAACCCACAAAATGCTTGTGATTTTATCATTAGTTTTGGCAAAGGAAACATCCATGCCTCCTCCAGACATTATGACAAGAATCACGGGCCCCTTGGATACGTCCGCAACTTCACTAACTAGTTGTTGTTGTTGACCTGGAAGTAGAATGTTAACTCTGTCGAGACTCTCCGCCTCTATAGCTAGGTTTGCACCTACAACAATAATGGTTGCATCTGCAGAGGCTGCAATCTTCGCAGCATCATCTATCTGGGCATTCGCGCATTGCACATCAGGACAACCAGGAGCGTAAGTCGTAGAAACCAAGGCCGTTAGGCCTTGCAATGGTGATGTATATTTGCAAGGGATGCCTTCATAGTTTCCAATCATGACCCTTGTAGCATTAGCATTAGGTCCTATAACTGCCAAAGATTTAATGGATTTGGAATTTAGAGGCAATGATCCTGGACTATTTTTAAGCAACACAATCCCTTGTCTTGCAGCTTCGCGAGCTAACTCTTGGTTCTCTGGAGTGCACACATCCTTTGGACCAAGATTTCCATAAGCTTGCTTGCTTGGATCACCATCAAAGAAACCAAGACGCATCAATGTTGCAAAATTGTTCGAGATAGCATTGTTAATGGACGCTTCATCAACAAGCCCTTGCTTTACCGCTCCTCCAGTATATTGACTTAGATAACTTCCACAGTCCAAATCTAAACCTGACAGTATGGTTTTGGCTGCAGCTTCTTCGGGAGTTTTGGTATAATGCTGATCTTTGAAAAGCACTTCCACTGAGTCACAATCAGAAACTATGTATCCATTTAATTTCCATTTTCCTCTGATAACTCCCTTAAGAAGATCAGGGTCTGCACAAGTTGGTTTTCCATTAACTTGATTGTAAGAACACATGACACTAGCTACATTCCCATCGATCACGCAACTCTTAAATGGAGGTTGGAATGTGTCATCCAAATCTTGTTGACTCACCACAGCATTGAACGTATAACGTTGAACACCTTTCCAATTATCAACATCGTAAGCGGTGTAATGTTTGCAACAAGCAGCAACCTTAAGCTTGTCGGAGTCTCCGTCATCGGTTTGTTGAAGACCTTTCACATATCCTGCGGCATATTTGCCACTTAACAAAGGATCTTCTCCCGGTGTTTCTTGTCCCCTTCCCCATCTCGGATCTCTGAATATGTTTATGTTTGGTGACCAATATGTCAAACCAGCCAAACCTACATTGTACATTGCTCTTGCTTCAGTTGAAACCACACTTCCAATGGCTTGAAAGAGGGTAGTATTGAAGGAAGCTGCAGTAAGAATAGGCATAGGAAAACTAGTAGCACCTGGAACCAAACTTGAAAAACGTGTACCAGGACCAATATTAGAAACTCCATGAAGAGCTTCACGACCACCATTCAT
TARGET=549,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2343
PRIMER_LEFT_PENALTY=0.132857
PRIMER_RIGHT_PENALTY=0.101479
PRIMER_LEFT_SEQUENCE=TGTACGAACCGAAAATCACATC
PRIMER_RIGHT_SEQUENCE=CAAACCTAGCTATAGAGGCGGA
PRIMER_LEFT=371,22
PRIMER_RIGHT=616,22
PRIMER_LEFT_TM=59.867
PRIMER_RIGHT_TM=59.899
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.8811
PRIMER_LEFT_1_PENALTY=0.132857
PRIMER_RIGHT_1_PENALTY=0.748212
PRIMER_LEFT_1_SEQUENCE=TGTACGAACCGAAAATCACATC
PRIMER_RIGHT_1_SEQUENCE=CTCTGCAGATGCAACCATTATT
PRIMER_LEFT_1=371,22
PRIMER_RIGHT_1=648,22
PRIMER_LEFT_1_TM=59.867
PRIMER_RIGHT_1_TM=59.252
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.8883
PRIMER_LEFT_2_PENALTY=0.706271
PRIMER_RIGHT_2_PENALTY=0.182061
PRIMER_LEFT_2_SEQUENCE=GATACGTCCGCAACTTCACTAA
PRIMER_RIGHT_2_SEQUENCE=CCTTGGTTTCTACGACTTACGC
PRIMER_LEFT_2=523,22
PRIMER_RIGHT_2=733,22
PRIMER_LEFT_2_TM=59.294
PRIMER_RIGHT_2_TM=60.182
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.8883
PRIMER_LEFT_3_PENALTY=0.706271
PRIMER_RIGHT_3_PENALTY=0.182061
PRIMER_LEFT_3_SEQUENCE=GATACGTCCGCAACTTCACTAA
PRIMER_RIGHT_3_SEQUENCE=GCCTTGGTTTCTACGACTTACG
PRIMER_LEFT_3=523,22
PRIMER_RIGHT_3=734,22
PRIMER_LEFT_3_TM=59.294
PRIMER_RIGHT_3_TM=60.182
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.9475
PRIMER_LEFT_4_PENALTY=0.846067
PRIMER_RIGHT_4_PENALTY=0.101479
PRIMER_LEFT_4_SEQUENCE=ATGCCTCCTCCAGACATTATGA
PRIMER_RIGHT_4_SEQUENCE=CAAACCTAGCTATAGAGGCGGA
PRIMER_LEFT_4=481,22
PRIMER_RIGHT_4=616,22
PRIMER_LEFT_4_TM=60.846
PRIMER_RIGHT_4_TM=59.899
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=136
=
PRIMER_SEQUENCE_ID=SSR_04304 Ps_contig_mira-and-tgicl-ass_franssen_32814
SEQUENCE=GGTGTAAGAATAACAAAACTAGTATGGCTACTACTCATTTTCGCCGTGTTTTTGTACACTAAGGTGAATATCAAATGCCAAGTTTTGACAATGCTCGTCGGCAGTATCAAGTGACTTGCATTGCAAGGAACGGCATTCGTGATCCTCAGCGAGAGGAACTGACACCAACTGGGGTGCTTTTACTATTTTGTGTTCTACCGTGCCGAAGCTCATTCCATCTCCGAATGAGAAAACAGTCTGTCCTTTATAAAATCTATAAGTTCTTCCAGGGTAACCGGTAGCAGGATCCGCCCTCATGTTCATGTTCGTCATTGGTACCTTTTCTACATATGATTGCGGATACCATGTCATCGGTAGCCTTCCACTTGGATTGTACGAACCGAAAATCACATCGGCTATGGCAGCTCCACCGGCTTCGCCAGGGTAGCCAACCCACAAAATGCTTGTGATTTTATCATTAGTTTTGGCAAAGGAAACATCCATGCCTCCTCCAGACATTATGACAAGAATCACGGGCCCCTTGGATACGTCCGCAACTTCACTAACTAGTTGTTGTTGTTGACCTGGAAGTAGAATGTTAACTCTGTCGAGACTCTCCGCCTCTATAGCTAGGTTTGCACCTACAACAATAATGGTTGCATCTGCAGAGGCTGCAATCTTCGCAGCATCATCTATCTGGGCATTCGCGCATTGCACATCAGGACAACCAGGAGCGTAAGTCGTAGAAACCAAGGCCGTTAGGCCTTGCAATGGTGATGTATATTTGCAAGGGATGCCTTCATAGTTTCCAATCATGACCCTTGTAGCATTAGCATTAGGTCCTATAACTGCCAAAGATTTAATGGATTTGGAATTTAGAGGCAATGATCCTGGACTATTTTTAAGCAACACAATCCCTTGTCTTGCAGCTTCGCGAGCTAACTCTTGGTTCTCTGGAGTGCACACATCCTTTGGACCAAGATTTCCATAAGCTTGCTTGCTTGGATCACCATCAAAGAAACCAAGACGCATCAATGTTGCAAAATTGTTCGAGATAGCATTGTTAATGGACGCTTCATCAACAAGCCCTTGCTTTACCGCTCCTCCAGTATATTGACTTAGATAACTTCCACAGTCCAAATCTAAACCTGACAGTATGGTTTTGGCTGCAGCTTCTTCGGGAGTTTTGGTATAATGCTGATCTTTGAAAAGCACTTCCACTGAGTCACAATCAGAAACTATGTATCCATTTAATTTCCATTTTCCTCTGATAACTCCCTTAAGAAGATCAGGGTCTGCACAAGTTGGTTTTCCATTAACTTGATTGTAAGAACACATGACACTAGCTACATTCCCATCGATCACGCAACTCTTAAATGGAGGTTGGAATGTGTCATCCAAATCTTGTTGACTCACCACAGCATTGAACGTATAACGTTGAACACCTTTCCAATTATCAACATCGTAAGCGGTGTAATGTTTGCAACAAGCAGCAACCTTAAGCTTGTCGGAGTCTCCGTCATCGGTTTGTTGAAGACCTTTCACATATCCTGCGGCATATTTGCCACTTAACAAAGGATCTTCTCCCGGTGTTTCTTGTCCCCTTCCCCATCTCGGATCTCTGAATATGTTTATGTTTGGTGACCAATATGTCAAACCAGCCAAACCTACATTGTACATTGCTCTTGCTTCAGTTGAAACCACACTTCCAATGGCTTGAAAGAGGGTAGTATTGAAGGAAGCTGCAGTAAGAATAGGCATAGGAAAACTAGTAGCACCTGGAACCAAACTTGAAAAACGTGTACCAGGACCAATATTAGAAACTCCATGAAGAGCTTCACGACCACCATTCAT
TARGET=971,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1241
PRIMER_LEFT_PENALTY=0.077340
PRIMER_RIGHT_PENALTY=0.046801
PRIMER_LEFT_SEQUENCE=AACTCTTGGTTCTCTGGAGTGC
PRIMER_RIGHT_SEQUENCE=TGTGACTCAGTGGAAGTGCTTT
PRIMER_LEFT=919,22
PRIMER_RIGHT=1208,22
PRIMER_LEFT_TM=59.923
PRIMER_RIGHT_TM=59.953
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=290
PRIMER_PAIR_PENALTY_1=0.2235
PRIMER_LEFT_1_PENALTY=0.176730
PRIMER_RIGHT_1_PENALTY=0.046801
PRIMER_LEFT_1_SEQUENCE=CCTTTGGACCAAGATTTCCATA
PRIMER_RIGHT_1_SEQUENCE=TGTGACTCAGTGGAAGTGCTTT
PRIMER_LEFT_1=947,22
PRIMER_RIGHT_1=1208,22
PRIMER_LEFT_1_TM=60.177
PRIMER_RIGHT_1_TM=59.953
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.2778
PRIMER_LEFT_2_PENALTY=0.077340
PRIMER_RIGHT_2_PENALTY=0.200424
PRIMER_LEFT_2_SEQUENCE=AACTCTTGGTTCTCTGGAGTGC
PRIMER_RIGHT_2_SEQUENCE=GCATTATACCAAAACTCCCGAA
PRIMER_LEFT_2=919,22
PRIMER_RIGHT_2=1176,22
PRIMER_LEFT_2_TM=59.923
PRIMER_RIGHT_2_TM=60.200
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.2991
PRIMER_LEFT_3_PENALTY=0.077340
PRIMER_RIGHT_3_PENALTY=0.221785
PRIMER_LEFT_3_SEQUENCE=AACTCTTGGTTCTCTGGAGTGC
PRIMER_RIGHT_3_SEQUENCE=TATACTGGAGGAGCGGTAAAGC
PRIMER_LEFT_3=919,22
PRIMER_RIGHT_3=1091,22
PRIMER_LEFT_3_TM=59.923
PRIMER_RIGHT_3_TM=59.778
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=173
PRIMER_PAIR_PENALTY_4=0.3023
PRIMER_LEFT_4_PENALTY=0.077340
PRIMER_RIGHT_4_PENALTY=0.224933
PRIMER_LEFT_4_SEQUENCE=AACTCTTGGTTCTCTGGAGTGC
PRIMER_RIGHT_4_SEQUENCE=GATTGTGACTCAGTGGAAGTGC
PRIMER_LEFT_4=919,22
PRIMER_RIGHT_4=1211,22
PRIMER_LEFT_4_TM=59.923
PRIMER_RIGHT_4_TM=59.775
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_04305 Ps_contig_mira-and-tgicl-ass_franssen_32816
SEQUENCE=ATCAGAGAGAGAGGAGCCTCAGTCGGCAGGCGACGAAAGTGGAGACTGCAGAAAAAGGTGGGGAGCGGAGACCGAAGCCGGCTCCGATAAGCTCTACGGCGGAGGAGGTTACGAGAAAGGCGGGGTTAGGGTTCCGGCTATGCGAGGTGGTGGTGTGTCTGATTTCGTTTTCCGTTATGGCTGCAAATAAGACGCAAGGTTGGAGTGGGGACTCTTATGATCGTTATAAAGAATACAGGTATTGTTTATCAATGAATGTTATCGGATTTGCATACTCAGGGTTACAAGCTTGTGATCTAGCCTTTCAACTAGTCACAGGAAAACACATGATCAGTCACCACCTTCGCCACCATTTCCAGTTCTTCATGGATCAGGTTGTGGCATATCTTCTGATATCAGCATCATCCTCTGCAGCCACAAGGGTGGATGATTGGCAATCAATTGGGGAAANTGAGTTCACTGAGATGGCTACTGTTTCAGTTGGAATGTCTATTTCNGGTCTTTTGTGCTATTTGACTATGAGCTCACTCATCTCTGTTACATTCCTATACGGTAAACCG
TARGET=4,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04306 Ps_contig_mira-and-tgicl-ass_franssen_32818
SEQUENCE=TTGTTCTTGAGCTCAGTAGCCAAAACAGCACCTCTTTGAGTCTCCAACTTCTGTCTCCAATCAACACCGGAATACTTTGGATCAAACTCATTCAAAGCATTCACAGTAAGGAATGACCTTTGCTTATTCAATTCAACAACACTGTGCACCTCACACCTAGCAACAAGATACATATCATTATCAAGCTTCCACCTTCTATACCTATAAGCAACAGAAGCAACCTCTTCACCCTCATTAGCAAAAGGGTTAGGCTCGTCAAAAGTAACCTTGTTCCCATCCCTAATCAACACTTGCTGAGAGAAGTTTTGATTGATATAAGCAGCTTCAACACTCAAAGAATGAGCAGAGTTAATATCATCTTTAGCTTCAGGCAAAGGCTCCTGCGAGGTCTCGTGAACAGAAAGCAAATCCAACTGCGACCCATCACGCTTATCAAAGAACAACTTATTCCCAACGCGCTGAACAACAATATCCCAAGAGTAAACAGACCTGGGAGCACACATGAGAGTCGACAAAATAGTATCAGTTGCAAAAACTGTTGCTTTATCCTCATTAGCGAGCCTCCTGATAACAGGGTCATCCGTGGTTGTAACCTTGAAGAAGTTACGATTCTTGAACCTCTCTAGACGACGCTCATTTTTCGGGTTGATTCGGTCAAAGCTACGATCATAACTCTCAAGAGCACCACATAGTAGAAGATCTTCAGGTTCAGGAACATTGTAGGATAGCTTGGTGAAAGTCGAAAACGGGATCTGATCATGCATATTCCACTCCGGCTGAATATCAACCGACGATTTGCAAACTGCAGCCTCACGCCGGGGATTGTTAGGGTTTGAACGGTTCTGATGGTAAAGACGGTCACGACGAGCTCTCTCCTTCTCCGCCTCACGTTTCTTTGCTTCCACTTCTTCATCACGACGCTGAGGGAGTTGTCGTTGTTGCTGGTATCTCCATTTAGGGCCGAATTTGGGACGCGGTGGTGGTTTACCATCGACTAGACGGAATGTGGCGTCGTCGNCGTTGCCAGGGAAGGAATCGTCGAGGGTGGAAATCAAAGGCGGAATCAGCAGGGTTTTTGGATCGGGTCGGGTTGTTGAAGTTGCGGGTCCAATCCGCGATTCGACCTAGTTTGTCGGAGCGGGAGAAAGGGGCGAAGGGGACATTGAGAGGGAGGTTGTTGTTGGCGGCGGCGGCGGTGGTGGAATCCATTGGGCCCCATCCGTCGGGGTTGAAAGGAACGGCTCCGACATCGAATGCAACTCCTACCATTGGTGCAAGGTGGTGGTRGGGATGATGAGTCTGTGGAGTACTCAGTGGTGTAAACTGGTTTGAGAGACGATGATGAAAGAGATGAATGAGTCAAAACCCTC
TARGET=1183,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04307 Ps_contig_mira-and-tgicl-ass_franssen_32822
SEQUENCE=GTCGGGCGTTGTTAATGGGAGCAGATTATTATGAGACGGATGCTGATAGGAGGTTTTTGGCTGCTAAAGGCGGTGTTCCAATCGGTATTGGCAAGAATTCTCATATTAAAAGGGCAATCATTGACAAGAATGCTAGAATTGGCGACGATGTCAAGGTTATTAACAGAATCAGAAGTTGAAAAGAGAAGAGAGAGAGAAATGGAGGGTGGAGATGAAGCTCTGGAGATGGTAGTTGACTCCAAAGACATGCAACAGCAGAGTAAAGCCTTCGACAAACTCACTGACCGCGTCGAAGATCGCCAGCTTGATTCCACTCGCGTTCAAGAAGCCATGGCTTCCATTGCTGCTTCTGCTGAAGCCGATTGGAATGCCATGCGCTTGAGGGAGAAAGAATTAGCCGCTGTTAAGATAAATGCAGCAGATGTTGATATAATTGCAAACGAACTAGAGTTGGACAAAAAGGTGGCTGAAAGAACCCTGCGTGAGCATAAAGGTGATGCTGTTGCTGCCATTCGTCATTTGCTTCACTAGTGTGGTAGAAACAAACAAGGGAATATGATCTGTATTGGCTTATATTTACTATTTTGCATTGAAATTTGAATCTCAAATGTATACTATGATGATGATGATATAGTTCATTAGAAATCTGAACTATGATGTTGATGATATTGGAATAAGCCTCACCCCCTCAATTTTTGTGGTCCAAATGAAGCTCCTATTAGCTTACGGAGTTTGGTTATTCACGAAGATCTTCTTGCTTGTTGGACTTATATAGTTTGTCATCATCAAGCTAAGCTAGTACTAGTTTCTACTCAAATGTAACAGCCCTACACGCTTTTGTGATTACATTATGTTGCTTCTGACGATCCAACAATTATGGGACTTGCTCTCTATGTGTGATCAAATATAAGAATTTCTTAATCGAC
TARGET=187,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1158
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.081670
PRIMER_LEFT_SEQUENCE=GATGCTGATAGGAGGTTTTTGG
PRIMER_RIGHT_SEQUENCE=GTCGAAGGCTTTACTCTGCTGT
PRIMER_LEFT=38,22
PRIMER_RIGHT=272,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=0.1158
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.081670
PRIMER_LEFT_1_SEQUENCE=GGATGCTGATAGGAGGTTTTTG
PRIMER_RIGHT_1_SEQUENCE=GTCGAAGGCTTTACTCTGCTGT
PRIMER_LEFT_1=37,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.082
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.1878
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.153708
PRIMER_LEFT_2_SEQUENCE=GATGCTGATAGGAGGTTTTTGG
PRIMER_RIGHT_2_SEQUENCE=ATCTCCAGAGCTTCATCTCCAC
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=226,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.846
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.1878
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.153708
PRIMER_LEFT_3_SEQUENCE=GGATGCTGATAGGAGGTTTTTG
PRIMER_RIGHT_3_SEQUENCE=ATCTCCAGAGCTTCATCTCCAC
PRIMER_LEFT_3=37,22
PRIMER_RIGHT_3=226,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.846
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=190
PRIMER_PAIR_PENALTY_4=0.3240
PRIMER_LEFT_4_PENALTY=0.242357
PRIMER_RIGHT_4_PENALTY=0.081670
PRIMER_LEFT_4_SEQUENCE=GATAGGAGGTTTTTGGCTGCTA
PRIMER_RIGHT_4_SEQUENCE=GTCGAAGGCTTTACTCTGCTGT
PRIMER_LEFT_4=44,22
PRIMER_RIGHT_4=272,22
PRIMER_LEFT_4_TM=59.758
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_04308 Ps_contig_mira-and-tgicl-ass_franssen_32822
SEQUENCE=GTCGGGCGTTGTTAATGGGAGCAGATTATTATGAGACGGATGCTGATAGGAGGTTTTTGGCTGCTAAAGGCGGTGTTCCAATCGGTATTGGCAAGAATTCTCATATTAAAAGGGCAATCATTGACAAGAATGCTAGAATTGGCGACGATGTCAAGGTTATTAACAGAATCAGAAGTTGAAAAGAGAAGAGAGAGAGAAATGGAGGGTGGAGATGAAGCTCTGGAGATGGTAGTTGACTCCAAAGACATGCAACAGCAGAGTAAAGCCTTCGACAAACTCACTGACCGCGTCGAAGATCGCCAGCTTGATTCCACTCGCGTTCAAGAAGCCATGGCTTCCATTGCTGCTTCTGCTGAAGCCGATTGGAATGCCATGCGCTTGAGGGAGAAAGAATTAGCCGCTGTTAAGATAAATGCAGCAGATGTTGATATAATTGCAAACGAACTAGAGTTGGACAAAAAGGTGGCTGAAAGAACCCTGCGTGAGCATAAAGGTGATGCTGTTGCTGCCATTCGTCATTTGCTTCACTAGTGTGGTAGAAACAAACAAGGGAATATGATCTGTATTGGCTTATATTTACTATTTTGCATTGAAATTTGAATCTCAAATGTATACTATGATGATGATGATATAGTTCATTAGAAATCTGAACTATGATGTTGATGATATTGGAATAAGCCTCACCCCCTCAATTTTTGTGGTCCAAATGAAGCTCCTATTAGCTTACGGAGTTTGGTTATTCACGAAGATCTTCTTGCTTGTTGGACTTATATAGTTTGTCATCATCAAGCTAAGCTAGTACTAGTTTCTACTCAAATGTAACAGCCCTACACGCTTTTGTGATTACATTATGTTGCTTCTGACGATCCAACAATTATGGGACTTGCTCTCTATGTGTGATCAAATATAAGAATTTCTTAATCGAC
TARGET=617,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6171
PRIMER_LEFT_PENALTY=0.130883
PRIMER_RIGHT_PENALTY=0.486257
PRIMER_LEFT_SEQUENCE=CCATTCGTCATTTGCTTCACTA
PRIMER_RIGHT_SEQUENCE=TCGTGAATAACCAAACTCCGTA
PRIMER_LEFT=508,22
PRIMER_RIGHT=745,22
PRIMER_LEFT_TM=60.131
PRIMER_RIGHT_TM=59.514
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.6441
PRIMER_LEFT_1_PENALTY=0.130883
PRIMER_RIGHT_1_PENALTY=0.513182
PRIMER_LEFT_1_SEQUENCE=CCATTCGTCATTTGCTTCACTA
PRIMER_RIGHT_1_SEQUENCE=ATAGGAGCTTCATTTGGACCAC
PRIMER_LEFT_1=508,22
PRIMER_RIGHT_1=718,22
PRIMER_LEFT_1_TM=60.131
PRIMER_RIGHT_1_TM=59.487
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.7117
PRIMER_LEFT_2_PENALTY=0.225403
PRIMER_RIGHT_2_PENALTY=0.486257
PRIMER_LEFT_2_SEQUENCE=GAGCATAAAGGTGATGCTGTTG
PRIMER_RIGHT_2_SEQUENCE=TCGTGAATAACCAAACTCCGTA
PRIMER_LEFT_2=483,22
PRIMER_RIGHT_2=745,22
PRIMER_LEFT_2_TM=59.775
PRIMER_RIGHT_2_TM=59.514
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=0.7117
PRIMER_LEFT_3_PENALTY=0.225403
PRIMER_RIGHT_3_PENALTY=0.486257
PRIMER_LEFT_3_SEQUENCE=TGAGCATAAAGGTGATGCTGTT
PRIMER_RIGHT_3_SEQUENCE=TCGTGAATAACCAAACTCCGTA
PRIMER_LEFT_3=482,22
PRIMER_RIGHT_3=745,22
PRIMER_LEFT_3_TM=59.775
PRIMER_RIGHT_3_TM=59.514
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=0.7386
PRIMER_LEFT_4_PENALTY=0.225403
PRIMER_RIGHT_4_PENALTY=0.513182
PRIMER_LEFT_4_SEQUENCE=GAGCATAAAGGTGATGCTGTTG
PRIMER_RIGHT_4_SEQUENCE=ATAGGAGCTTCATTTGGACCAC
PRIMER_LEFT_4=483,22
PRIMER_RIGHT_4=718,22
PRIMER_LEFT_4_TM=59.775
PRIMER_RIGHT_4_TM=59.487
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_04309 Ps_contig_mira-and-tgicl-ass_franssen_32827
SEQUENCE=TTATGCTTTGAAATAAACTACATGGCTCATGTAGNTAGTAATAATAAGAAGGCATTTCTATAAAACTACTCCTGTCTTTTTGGAAATGTGAAGGTTCAGGCCGGTGATAAACATTGCGGATATACAGACAAGTTAGCATCATACAGTTAACGTACTGGCAGAATATCACTGCCGCAATGCAACCGGTCAATCAATTATACAACGAGAAAGAAAGGAAAGAAGAAAAAAAAGACCAAGACCAAATGCAGGAACTTCTATGGGGAATGGATATAACCAAATAGGAAAGATGTAAAAGCAGCTTTGATTCTGTAAATCTTTCCGAAAGCACATTCAATTTCTTGAACCATTTAGGCCATCACCAATGGCAGCTCAATTCTAGTACAAGGTGACACCAAATTGAATGTGTTCCAACTAAAAAAAGTCTTGTTTTTTGGGACACTCTGATGCCCGATGACCTGATTGGCCGCAATTAAAACAGGCCCCTCTGTTTAACGATGAAGATCTGTTTGGCGATGAAGACCTGTTTGTTGACGAATATCCGCTTGACCGTCTACCTCCACTTGACCAGTCATCCCCATCTGATCGTCTGCCTCCACTTGACCAGTCATCCCCACTTGACCAGTCATCCCCACCTGATCGTCTACCTCCACTTGACCAGGCATCCCCACCTGATCGTCTACCTCCACTTGACCAGGCATCCCCACCTGATCGTCTACCTCCACTTGACCAATCATCCCCACCTGATCGTCTAACTCCACTTGACCAGGCATCCCCACCGGTTCTATAACTTCTCTGATCCCTAGAACCTCTCGGAGTACTTCTATCTCTATCAAGTGTATCAACACCTTGCCTGATAGATATTTCTGATAAATCTTGCATAGGTGGTGGACTAATAAATTCAAACCTGGAGCCTATATCACGCTCAAAGGACATAACAGTTCCTTTCTGGCTAGCGGTGTACATTACTACAGCAGCACCCTCTTTTCCAGCACGTCCAGTACGACCAGAGCGGTGAACAAATATCTCAGGACTATCGGGAAGTTCATAGTGTATAATCAAATCGACATTGGGAATATCTAGTCCACGAGAAGCAACATCAGTAGCAACAAGCACTGTGAATCTCCCTTGCCGAAAACCATTCAATGTTCTCTCTCTCTGATACTGAGAGATATCACCATGCAGTGCTTTAGAAGCAATACTATTTGATAATGACAGTGATATTTCATCAGCATCCCTTTTTGTCCGTGTGAAAACAATAGTCTTCCCACCCTTTCCATAGGCATTTATAAGACCATAAAGAATTCTTCGCTTTGATGACGCAGTGGCTGATACAGCATAAAGCTTGATCCCCTCAGCAAGCTTTTCTTCTCTATCACCGACCAAATCAATAGTCAATGGATTGTTCAAATGTTTTCTTGACAACTTCTTGACCCAACCCGGCATGGTTGCAGAGAAAAGCATGATCTGCCGCTGAGACGGGATCGTTTCTAAAATCACTTCCACATCCTCCTCAAACCCAACAGCAAGCATCTGATCGGCTTCATCAAGTACCAAGTACTGAATTTCACTAAGTTTAAGGCTTTTCCCATTTATCAAGTCAATTAGTCTCCCAGGTGTTCCAACCACAACATCAACACCACGYGAAAGCGCATTTTTCTGAATATCATAAGAAACCCCACCATAAACACAAACAGTGTTTAGATAAGGTGCAGATTCTTGTATCTCCTTCTCTACCTGCTTTGCTAACTCCCTAGTTGGTGCAAGGACCAGCACTTTAGGAAGCCGCCCAGATCGCCTCATAGTGCTTTGTCCATTCTCAGTGAGACCCTTGATAATTGGAATTCCAAATGCTAGCGTCTTACCGGTCCCGGTCCTTGCACGAGCAATGATATCTCTACCTTCTATTGCAGGCAGCAACACAGCTCTCTGAATGGGGAAAAGATTGGTAATTCCTCGCCTTCGGAGGGAGTCCACAAGTTGAGAAGGAAAACCAAGTTTGGAAATGTCGAGTTCGTCTTTGCTGACGGCAACAGGAACGTGGCTGAGTTGGTCGTTAGAGTCGTCATCGAGGTTAAAACCCTTGAAGGCTTCTTGAGCGGTGAGGATGGAAGTGTTGGGAGTGGCAACTGCGGAAATAACGAAGGAAACGGCGGGGGTTTTGTGGTTGGGTCGGGGTCGGAGTCGGAGAAGGGTGTTGAAACGGGAAGTGTTGTGAATTGAAAAGGAGGCTGGGGTTTTAGGAAGTTGCAGAGAGGAAGAAGATCCAATTGTACCGGAAATGGACGCCATTTCGTGTTTTAGGTTTGGGTTCGAGAGGAGAGAAAATGGACGGCAAGAACAAGATAAGGCAGCACCCTCTCGAAGTTGAGTAAGATGGCCTTTTAAACCTCCACGTCCTTGTTCCTCTTCTTTCTGTGGCTGTNT
TARGET=1147,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04310 Ps_contig_mira-and-tgicl-ass_franssen_32831
SEQUENCE=ATAGATAGACAAGTGAANCYAATGAATTGCACTATCCAATGTTTTAGTAAAAAGATCACGAGTTTAACTCTCCCAATGACATATCACACACACACACAAACTAATGTTTGGTTTTACTACTTAAAATTACATACATATATTTTGGGAAAGAGAAGATAGAAATAAACCGGTACACACTCATGTTTACACGAAAAATGAATACAGAGACAGAAAAAAATGATCTGCTACAAGTTGTAACTGTCAATTCCCAAATCCCTCAAGGCATCAGTAGCAGCTTCCTTACAGTTTGCATGTGTTTGCTTTGCCTTCCTTATGTATTTCTCAATTCCATTATTAAGAAGAATAGTTCTGTTCTCTAGGTTTCTCGCTACTAAATTTCTGATCATAAGACAGGAGTTTCTTTGCATTTGGTGTGCTGCGGGAAATTTCTGCATTGATTGAATAGCAAGGTCACCGGCACCAGCTTCAATGGCTCGGGCTGCATTTTCCGGGGATCTTAGGGAAAGCACAGAAATAATAGACATGACCTCTTGCAAAACAGAAGGATCATCAGCATATCTGGCTGAAAGCTTGATTAGCTTATCCATACCCCCTTTCCCAACAATTGCACTCTTGTTTGCATCACTTCCTGCTAACTTTGAAAGTAATGAGCAGCAAACTTTGGCTACATCTGTGTTGCCTTGTTCACCACTGTCATCTATGCATTTGAGTACTACATCAATACCACCTTTTTCAGCAATGGATTTACATATTTCATCATTTACTGCAGTGGCCTTTAAAGTGATGCATGCTGAAATTAGGTCAGGTGCGCCAAGTCCTGTGTGCAGAGAATCCACAATGGCTTCTGGAATTCCAATCTTAGCAAATTTTCGTGCATATCCATAAACTTGGGAAGCTAGAACCCGATTGT
TARGET=85,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04311 Ps_contig_mira-and-tgicl-ass_franssen_32836
SEQUENCE=TTTGGCTAAAACTCACTAATCTACTTGAATACAATCACTTACTCATAGAAGAAGTTGCTTTGCAACATTACATATTTATATATCTCGCATGTTGTACTTAAATGTTACATTAACAAAAGCAGCATTTTTTCGCTAGATATACATATATGCGGATGGATCCAAACCAGGACATCACATTTCTATACATCTTGTGTTTGGACAAGAATCAATGGAAATAACTCACGTTGCGCTATTCCGTCTTCGATTTGGCGAGCGCTTTCTTCCTCTGTGCAAGCATGTATGAGTACATGTGAGGACTACCTGGAACGTAGATTGCCAACGCAATAATTGCAATGTAAAAGAAATCGAATGAAGAGTTCCATGTATTAGGCATTCTTATGCAATACTTCTCAGATGCCTTAATGAATGGCAAGGCAATGTATATCAAACCAACTTCACTGCTTATGCCTGTTGGATACAATAGTAAGAACGTGCTATATCTAAGCCACAAAAACCAAGATGGGGTGAATCCAAAAGCCTCTTTAAATCCAAAGAAAGAATAACGAATAATCTCTGTGATAGACCAGCTGATTAGTAGGGAGCTCACAAGAACATGAGTTCGAGTCTCAGGAAAGCTCCACAAGATACCCCATACAAGAAACAGCCTTGAACCAATCTGAGGCAATGTTGCTGTTATTGGAGACCTCACAAGCCCCACTAAACCATGAAGAATCTCTAATACAGCAGCGGTTTGAGCAAAAAGCAAGGGTTTTTCAGCTGCAGTGTAAACATGTTCATGACCCAATTCCTTCAATGTTTTCAAAGACAGATACAGAACTTGAAGCCATCCAAATAAAACAGTCCAATTGTAAAGAATAAGATAGAATCTTCTAATAGGTGAAGAAGAAGAAGAAGAAGTAGTAGCCATTGAAGGAAGTGATGAAGAGAGATCTAGGAGGAGAAACCCAAGAAGGGTTCAAAATTTGTTAAAATTGAAAGGGAAAAATTTAGAATAATTAATGAATGAATTAAAGGGTGTGGAATTTTGAAAATAAATTGTGTTATGATATTGGGTTATATAGGGTAAAGGGATTAATATGTGAGTGAGGAGGTAGCCCAACGATCCACAACTTTATTAATGAGAAATACATGTATAGAGAAGAATCCCCGAC
TARGET=879,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2968
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.292414
PRIMER_LEFT_SEQUENCE=GTTATTGGAGACCTCACAAGCC
PRIMER_RIGHT_SEQUENCE=TCATCACTTCCTTCAATGGCTA
PRIMER_LEFT=671,22
PRIMER_RIGHT=921,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.708
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.3526
PRIMER_LEFT_1_PENALTY=0.060196
PRIMER_RIGHT_1_PENALTY=0.292414
PRIMER_LEFT_1_SEQUENCE=ATGTTCATGACCCAATTCCTTC
PRIMER_RIGHT_1_SEQUENCE=TCATCACTTCCTTCAATGGCTA
PRIMER_LEFT_1=769,22
PRIMER_RIGHT_1=921,22
PRIMER_LEFT_1_TM=60.060
PRIMER_RIGHT_1_TM=59.708
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=153
PRIMER_PAIR_PENALTY_2=0.4002
PRIMER_LEFT_2_PENALTY=0.107763
PRIMER_RIGHT_2_PENALTY=0.292414
PRIMER_LEFT_2_SEQUENCE=TACCCCATACAAGAAACAGCCT
PRIMER_RIGHT_2_SEQUENCE=TCATCACTTCCTTCAATGGCTA
PRIMER_LEFT_2=624,22
PRIMER_RIGHT_2=921,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=59.708
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=0.4305
PRIMER_LEFT_3_PENALTY=0.138114
PRIMER_RIGHT_3_PENALTY=0.292414
PRIMER_LEFT_3_SEQUENCE=GCAATGTTGCTGTTATTGGAGA
PRIMER_RIGHT_3_SEQUENCE=TCATCACTTCCTTCAATGGCTA
PRIMER_LEFT_3=660,22
PRIMER_RIGHT_3=921,22
PRIMER_LEFT_3_TM=60.138
PRIMER_RIGHT_3_TM=59.708
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.5178
PRIMER_LEFT_4_PENALTY=0.225403
PRIMER_RIGHT_4_PENALTY=0.292414
PRIMER_LEFT_4_SEQUENCE=TCTGAGGCAATGTTGCTGTTAT
PRIMER_RIGHT_4_SEQUENCE=TCATCACTTCCTTCAATGGCTA
PRIMER_LEFT_4=654,22
PRIMER_RIGHT_4=921,22
PRIMER_LEFT_4_TM=59.775
PRIMER_RIGHT_4_TM=59.708
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_04312 Ps_contig_mira-and-tgicl-ass_franssen_32839
SEQUENCE=CTTAACTAAAAAACCAAAAACCAAAACTTTAAAAAAAAAATTAAAAAAAAAAGTAAAACGAAGACCCCCGGAAACCCCGAAAACTAATTAAATTACTAAACCAAACTTATCGAATAATATATNTGTAAGAATCCCCTCGATCTTCTTTNTGCATGGCTTTCTTTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATTGACTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTAGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGGTGATAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGAGGTGGTGGAGGCAAAGAAGCAAGTGGTGTCAGGGATGAAGTATCATCTCAATATTTCAGCCGTTGATCATAATGGTGTTCGCAGAATGTTCACCTCCGTCGTGGTGGTCAAGCCGTGGATTCAGTACAAAAAGGTTCTCCATTTTGGGGATTCATCTAGCTTCCACCAACTACAACATACTAGTATGTAAAAAGAGAGAGCTATATATATACGACAGGGAATCGTTTATTTGGACCTAAATAATACTAATAGTTAAGCAGTATATGGTTTCCTGACTATAGACGCGATTATTTGTAATTGCAACTGAGCTTGTTACTATTTTTCCTTTACAAAATTTTAAA
TARGET=282,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3531
PRIMER_LEFT_PENALTY=0.066394
PRIMER_RIGHT_PENALTY=0.286660
PRIMER_LEFT_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_RIGHT_SEQUENCE=GCCATACAAGTATCCATCACCA
PRIMER_LEFT=154,22
PRIMER_RIGHT=370,22
PRIMER_LEFT_TM=59.934
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.4350
PRIMER_LEFT_1_PENALTY=0.066394
PRIMER_RIGHT_1_PENALTY=0.368624
PRIMER_LEFT_1_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_RIGHT_1_SEQUENCE=ATGTTCCTACCCCAGTTTACCA
PRIMER_LEFT_1=154,22
PRIMER_RIGHT_1=317,22
PRIMER_LEFT_1_TM=59.934
PRIMER_RIGHT_1_TM=59.631
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.4350
PRIMER_LEFT_2_PENALTY=0.066394
PRIMER_RIGHT_2_PENALTY=0.368624
PRIMER_LEFT_2_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_RIGHT_2_SEQUENCE=TGTTCCTACCCCAGTTTACCAT
PRIMER_LEFT_2=154,22
PRIMER_RIGHT_2=316,22
PRIMER_LEFT_2_TM=59.934
PRIMER_RIGHT_2_TM=59.631
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=0.4350
PRIMER_LEFT_3_PENALTY=0.066394
PRIMER_RIGHT_3_PENALTY=0.368624
PRIMER_LEFT_3_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_RIGHT_3_SEQUENCE=CATGTTCCTACCCCAGTTTACC
PRIMER_LEFT_3=154,22
PRIMER_RIGHT_3=318,22
PRIMER_LEFT_3_TM=59.934
PRIMER_RIGHT_3_TM=59.631
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.4931
PRIMER_LEFT_4_PENALTY=0.066394
PRIMER_RIGHT_4_PENALTY=0.426682
PRIMER_LEFT_4_SEQUENCE=GGCTTTCTTTGCTTTCACACTT
PRIMER_RIGHT_4_SEQUENCE=CTCACACCTACCCTCCTCATGT
PRIMER_LEFT_4=154,22
PRIMER_RIGHT_4=335,22
PRIMER_LEFT_4_TM=59.934
PRIMER_RIGHT_4_TM=60.427
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_04313 Ps_contig_mira-and-tgicl-ass_franssen_32839
SEQUENCE=CTTAACTAAAAAACCAAAAACCAAAACTTTAAAAAAAAAATTAAAAAAAAAAGTAAAACGAAGACCCCCGGAAACCCCGAAAACTAATTAAATTACTAAACCAAACTTATCGAATAATATATNTGTAAGAATCCCCTCGATCTTCTTTNTGCATGGCTTTCTTTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATTGACTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTAGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGGTGATAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGAGGTGGTGGAGGCAAAGAAGCAAGTGGTGTCAGGGATGAAGTATCATCTCAATATTTCAGCCGTTGATCATAATGGTGTTCGCAGAATGTTCACCTCCGTCGTGGTGGTCAAGCCGTGGATTCAGTACAAAAAGGTTCTCCATTTTGGGGATTCATCTAGCTTCCACCAACTACAACATACTAGTATGTAAAAAGAGAGAGCTATATATATACGACAGGGAATCGTTTATTTGGACCTAAATAATACTAATAGTTAAGCAGTATATGGTTTCCTGACTATAGACGCGATTATTTGTAATTGCAACTGAGCTTGTTACTATTTTTCCTTTACAAAATTTTAAA
TARGET=338,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3718
PRIMER_LEFT_PENALTY=0.368624
PRIMER_RIGHT_PENALTY=0.003166
PRIMER_LEFT_SEQUENCE=ATGGTAAACTGGGGTAGGAACA
PRIMER_RIGHT_SEQUENCE=GAACATTCTGCGAACACCATTA
PRIMER_LEFT=295,22
PRIMER_RIGHT=537,22
PRIMER_LEFT_TM=59.631
PRIMER_RIGHT_TM=60.003
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.3718
PRIMER_LEFT_1_PENALTY=0.368624
PRIMER_RIGHT_1_PENALTY=0.003166
PRIMER_LEFT_1_SEQUENCE=TGGTAAACTGGGGTAGGAACAT
PRIMER_RIGHT_1_SEQUENCE=GAACATTCTGCGAACACCATTA
PRIMER_LEFT_1=296,22
PRIMER_RIGHT_1=537,22
PRIMER_LEFT_1_TM=59.631
PRIMER_RIGHT_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.4298
PRIMER_LEFT_2_PENALTY=0.426682
PRIMER_RIGHT_2_PENALTY=0.003166
PRIMER_LEFT_2_SEQUENCE=ACATGAGGAGGGTAGGTGTGAG
PRIMER_RIGHT_2_SEQUENCE=GAACATTCTGCGAACACCATTA
PRIMER_LEFT_2=314,22
PRIMER_RIGHT_2=537,22
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=60.003
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.5111
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.375090
PRIMER_LEFT_3_SEQUENCE=GTAAGGTGGAGGAGGTGATTTG
PRIMER_RIGHT_3_SEQUENCE=TGATACTTCATCCCTGACACCA
PRIMER_LEFT_3=226,22
PRIMER_RIGHT_3=491,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=60.375
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=0.5111
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.375090
PRIMER_LEFT_4_SEQUENCE=TAAGGTGGAGGAGGTGATTTGT
PRIMER_RIGHT_4_SEQUENCE=TGATACTTCATCCCTGACACCA
PRIMER_LEFT_4=227,22
PRIMER_RIGHT_4=491,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=60.375
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_04314 Ps_contig_mira-and-tgicl-ass_franssen_32839
SEQUENCE=CTTAACTAAAAAACCAAAAACCAAAACTTTAAAAAAAAAATTAAAAAAAAAAGTAAAACGAAGACCCCCGGAAACCCCGAAAACTAATTAAATTACTAAACCAAACTTATCGAATAATATATNTGTAAGAATCCCCTCGATCTTCTTTNTGCATGGCTTTCTTTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATTGACTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTAGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGGTGATAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGAGGTGGTGGAGGCAAAGAAGCAAGTGGTGTCAGGGATGAAGTATCATCTCAATATTTCAGCCGTTGATCATAATGGTGTTCGCAGAATGTTCACCTCCGTCGTGGTGGTCAAGCCGTGGATTCAGTACAAAAAGGTTCTCCATTTTGGGGATTCATCTAGCTTCCACCAACTACAACATACTAGTATGTAAAAAGAGAGAGCTATATATATACGACAGGGAATCGTTTATTTGGACCTAAATAATACTAATAGTTAAGCAGTATATGGTTTCCTGACTATAGACGCGATTATTTGTAATTGCAACTGAGCTTGTTACTATTTTTCCTTTACAAAATTTTAAA
TARGET=386,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2898
PRIMER_LEFT_PENALTY=0.286660
PRIMER_RIGHT_PENALTY=0.003166
PRIMER_LEFT_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_RIGHT_SEQUENCE=GAACATTCTGCGAACACCATTA
PRIMER_LEFT=349,22
PRIMER_RIGHT=537,22
PRIMER_LEFT_TM=59.713
PRIMER_RIGHT_TM=60.003
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.3718
PRIMER_LEFT_1_PENALTY=0.368624
PRIMER_RIGHT_1_PENALTY=0.003166
PRIMER_LEFT_1_SEQUENCE=ATGGTAAACTGGGGTAGGAACA
PRIMER_RIGHT_1_SEQUENCE=GAACATTCTGCGAACACCATTA
PRIMER_LEFT_1=295,22
PRIMER_RIGHT_1=537,22
PRIMER_LEFT_1_TM=59.631
PRIMER_RIGHT_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.3718
PRIMER_LEFT_2_PENALTY=0.368624
PRIMER_RIGHT_2_PENALTY=0.003166
PRIMER_LEFT_2_SEQUENCE=TGGTAAACTGGGGTAGGAACAT
PRIMER_RIGHT_2_SEQUENCE=GAACATTCTGCGAACACCATTA
PRIMER_LEFT_2=296,22
PRIMER_RIGHT_2=537,22
PRIMER_LEFT_2_TM=59.631
PRIMER_RIGHT_2_TM=60.003
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.4298
PRIMER_LEFT_3_PENALTY=0.426682
PRIMER_RIGHT_3_PENALTY=0.003166
PRIMER_LEFT_3_SEQUENCE=ACATGAGGAGGGTAGGTGTGAG
PRIMER_RIGHT_3_SEQUENCE=GAACATTCTGCGAACACCATTA
PRIMER_LEFT_3=314,22
PRIMER_RIGHT_3=537,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=60.003
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.4299
PRIMER_LEFT_4_PENALTY=0.286660
PRIMER_RIGHT_4_PENALTY=0.143280
PRIMER_LEFT_4_SEQUENCE=TGGTGATGGATACTTGTATGGC
PRIMER_RIGHT_4_SEQUENCE=CTAGATGAATCCCCAAAATGGA
PRIMER_LEFT_4=349,22
PRIMER_RIGHT_4=605,22
PRIMER_LEFT_4_TM=59.713
PRIMER_RIGHT_4_TM=60.143
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_04315 Ps_contig_mira-and-tgicl-ass_franssen_32839
SEQUENCE=CTTAACTAAAAAACCAAAAACCAAAACTTTAAAAAAAAAATTAAAAAAAAAAGTAAAACGAAGACCCCCGGAAACCCCGAAAACTAATTAAATTACTAAACCAAACTTATCGAATAATATATNTGTAAGAATCCCCTCGATCTTCTTTNTGCATGGCTTTCTTTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATTGACTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTAGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGTATGGCTTCTTGTGTGGTGTTGGTGGTGGTGGTGGGGAGGTGATAGACAGGGTGAGGGTGAGGGTAGGTGTGCACTGGTGGAGGTGGTGGAGGCAAAGAAGCAAGTGGTGTCAGGGATGAAGTATCATCTCAATATTTCAGCCGTTGATCATAATGGTGTTCGCAGAATGTTCACCTCCGTCGTGGTGGTCAAGCCGTGGATTCAGTACAAAAAGGTTCTCCATTTTGGGGATTCATCTAGCTTCCACCAACTACAACATACTAGTATGTAAAAAGAGAGAGCTATATATATACGACAGGGAATCGTTTATTTGGACCTAAATAATACTAATAGTTAAGCAGTATATGGTTTCCTGACTATAGACGCGATTATTTGTAATTGCAACTGAGCTTGTTACTATTTTTCCTTTACAAAATTTTAAA
TARGET=649,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1867
PRIMER_LEFT_PENALTY=0.110942
PRIMER_RIGHT_PENALTY=0.075753
PRIMER_LEFT_SEQUENCE=CATAATGGTGTTCGCAGAATGT
PRIMER_RIGHT_SEQUENCE=AGGTCCAAATAAACGATTCCCT
PRIMER_LEFT=514,22
PRIMER_RIGHT=683,22
PRIMER_LEFT_TM=59.889
PRIMER_RIGHT_TM=60.076
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.2948
PRIMER_LEFT_1_PENALTY=0.110942
PRIMER_RIGHT_1_PENALTY=0.183834
PRIMER_LEFT_1_SEQUENCE=CATAATGGTGTTCGCAGAATGT
PRIMER_RIGHT_1_SEQUENCE=TCCAAATAAACGATTCCCTGTC
PRIMER_LEFT_1=514,22
PRIMER_RIGHT_1=680,22
PRIMER_LEFT_1_TM=59.889
PRIMER_RIGHT_1_TM=60.184
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.4508
PRIMER_LEFT_2_PENALTY=0.375090
PRIMER_RIGHT_2_PENALTY=0.075753
PRIMER_LEFT_2_SEQUENCE=TGGTGTCAGGGATGAAGTATCA
PRIMER_RIGHT_2_SEQUENCE=AGGTCCAAATAAACGATTCCCT
PRIMER_LEFT_2=470,22
PRIMER_RIGHT_2=683,22
PRIMER_LEFT_2_TM=60.375
PRIMER_RIGHT_2_TM=60.076
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.4672
PRIMER_LEFT_3_PENALTY=0.391473
PRIMER_RIGHT_3_PENALTY=0.075753
PRIMER_LEFT_3_SEQUENCE=CATCTCAATATTTCAGCCGTTG
PRIMER_RIGHT_3_SEQUENCE=AGGTCCAAATAAACGATTCCCT
PRIMER_LEFT_3=490,22
PRIMER_RIGHT_3=683,22
PRIMER_LEFT_3_TM=59.609
PRIMER_RIGHT_3_TM=60.076
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=0.5589
PRIMER_LEFT_4_PENALTY=0.375090
PRIMER_RIGHT_4_PENALTY=0.183834
PRIMER_LEFT_4_SEQUENCE=TGGTGTCAGGGATGAAGTATCA
PRIMER_RIGHT_4_SEQUENCE=TCCAAATAAACGATTCCCTGTC
PRIMER_LEFT_4=470,22
PRIMER_RIGHT_4=680,22
PRIMER_LEFT_4_TM=60.375
PRIMER_RIGHT_4_TM=60.184
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_04316 Ps_contig_mira-and-tgicl-ass_franssen_32840
SEQUENCE=ATAAAAAAAACAAATAAAAAAACCGTAAAATTCGATTCNCGARNGGTGTGACTCATCATCACCTCACCACCATCCAATCACTTTCACTACCATTTTCCACTGCAAACAATGCTGTGTTTGAAACCGCACCTCTCTTTTCCATTTCGCATCTCTTCATCACCATCAACCTCTCCAAAACACCTCACGCGCCACCTCCGTTCCTCGCCGTTCAGATCCACGGTCAAGATGATGTCAACCAAATCCAAAGCCATTGAACACATCGTCCTCTTCAAAGTCAAAGAAAACACCGAACCCTCCAAAGTAGCCTCCATGGTTAACGGACTCAGTTCCCTCGTTTCGCTAGATCAAGTCCTTCATCTTACCGCAGGCCCTATCCTGCGCAATCGCTCCACCTCGTTAACGTTCACGCACATGCTTCACAGTCGGTACAAGTCCAAAGAGGATCTCGAAACCTACTCCGCGCACCCGAGCCATCTTGGCGTCGTCCGGGGAAGCGTTCTCCCGATCGTTGATGATATCATGGCGGTGGATTGGGTTGCCGACGATGTTAACGGTGCTGATTCGGTTCTGGCGCCGGGTTCCGCGATTCGTGTGACGTTTTTGAAATTGAAGGAGGATGCTGTTAGCGATGAGGTTGTGGAGGTTATTAGAGGAATTCCGGAGAGTTTTAAACAGATTAAGGAACTGACTTGTGGAGAGAATTTCTCGCCGGCGAGAGCTAAGGGCTATTCGGTTGCTTCACTTGCTGTGTTTCCGGGGGAGAAGGAGTTGGAGGAAGTTGATTCGAATAAGGAGCTGGTGGAGTATCAGAAGGATAAAGTTAGGGATCGTGTGGAGAGTGTGGTGGTGGTTGATTATGTGGTGCCGCCGCCGCCTCCGCCGCAATCTGCAAGTCTTTGATTTGATTGATGATGAAGGTTATTTCTGTTCAATTGGTGTTTTTGAAATTTGAGTTAAATTATTATGGGATTTAATAAAATGCAATGTAGAACAATTGATACTTTCACTTCAGTGTATTGTTTTGTAGTCTTGTTCTCTCAATTGTAATGTTTGGTTATTACATTACATTCAGTGTAAGTAGTTTTAAGTTANGT
TARGET=864,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04317 Ps_contig_mira-and-tgicl-ass_franssen_32841
SEQUENCE=TTTANTTAGCACTTGCACACAACACCCAAACAACAGCAGTGAAGAGTCTTTTGATGGCTCACATAGCTTATTGTGAACTTGTGAAGCAGTGAAAATTTGTATGTATATCCCCCTCTGGAATAGGAATAAAAACTAATGCATGCTACAGCATCAATAATGTGAGTAATATCATGCATACTTCCAAATCCATTTCGGATGATTGATGATCTTCATGCACAATCCCTGCATTTATGCGTGCTTACTTTCCTTTGCATTTTAAAATAAAACCTATATTTGAAGTTTCACTCGATCAAAATATTTTTCTTGCTCTGAGGTAATTATACATAAGAAAGAAAACAATAATGCCACCAACAATGATCCCAGATGTAGTTAGCCAAAATGCAAACACACGTTCCTCAAGATAGGACTTCAGGTTCATGCCAAATATACCTGCCACAAGGGCGCCAACTGCCACACAGAATGTTCCAACCTGGAGAAGCAGTTCCACTCTACTAACTTCAAGTCTTCGTGAGCTCAAACTGACACCTATAGAATCCTCCATTTCCCTTGCAGAATCAAGAAGCCTTTCAGATTGACCATGACAAGATTCACATCTTTGGAGATAGTTTTCCAGAAGCATTTCAATTTCCTCCTCCTCCTCATCAGCAACCTGCTTTTCTAAGGGCACTGAGCATTCCACATTATTGTTTCCTTTATTAAGTGTACAGTTTCTTCCCATAATACACATCCGTCGTATTTCATTTGGGTCCTCCAGAAGGTCAAGAAGCATCTGTCGGAGAGCTCCTGCCCTCGAGCCTAACTCAACCAAAGTTTGTTTGCTAATACGAAGTTGCTCCAATATGTCTCCAGTTATCCGATTTGGCAACGCCTCAAGAAGAGCTTGAACACGAGGTTCTAAGTCCATCAGTCTCCGCTCCAAACGTTGTATTCTTGAAAGCAATGCTGCTTCGACAACCTCAATTTCAAATGGCATTGATGGCCCTCCATTGTTGTTCTTGGGGTTCAACCTAGGCAGCAATGTGTCTAGAAAAGCTTGCCCACCTTTACGATTATAGTCAAATATAAGCACACAATCTTGCATTGCTATTGCTCGTAGCGAACCCAGGTTTAGAAGTATAGCATACTCACGGACCAGCAAAGAAGGCATTGAATTTGTCAAAAACAGTGATGGATCCACACTTCTGACATCCCGGGGACGAAGGCCACTCGACTTCAATAATTGTCTCCTGTTGATTTTTCTAGTCGATACTGCCCCGTCTGATTTCACTTCTACAACTTCATAAACAGGTTCTCGAATTCCGAGAGAAGGAGAATCGCCGGAAGAGGTAGTTGCAATCCTCTGAGGCTCAATAGAAGGAGTTTGCCTCAGAGCCGGAGCATTCGAATCATCCAAATCTTCATCGGCGTCAGATGCGACGGATTCCGCCTCGCTCCACTGCTTCTCCTCCGTGGATCTACTGCATTTCACCGGCTTCAGAGCCGACGGTGACGTAACAGAGATCTTTCTCCGGCGAAGAAGAGTGAGAGAATCATGACTCCGTGTTTCAAGAGAACGGAATTCGGTAAGGTCGGAGAAGATGACGTAGTTGGTGCGCGAGAGTAAAGGTTGAAAACGAAGGTGCGTCGAAGTTGAAGTTAAAGTTAAAGCCATGTTANTTTAGTTCAGAAGAGACTACGGCGACGGTTACGGTGGTTATGCATGCCGGTAACAGAATGAGGTAGTTGGAGCTGTGTTTCACTCTGCGTAACGATTAAAACGAAACAGATTCATAGTTTATTCGAATTTGGATATTTTATTGTT
TARGET=627,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1106
PRIMER_LEFT_PENALTY=0.021195
PRIMER_RIGHT_PENALTY=0.089381
PRIMER_LEFT_SEQUENCE=CTTGCAGAATCAAGAAGCCTTT
PRIMER_RIGHT_SEQUENCE=TTGGAGCAACTTCGTATTAGCA
PRIMER_LEFT=545,22
PRIMER_RIGHT=837,22
PRIMER_LEFT_TM=60.021
PRIMER_RIGHT_TM=59.911
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.1106
PRIMER_LEFT_1_PENALTY=0.021195
PRIMER_RIGHT_1_PENALTY=0.089381
PRIMER_LEFT_1_SEQUENCE=CTTGCAGAATCAAGAAGCCTTT
PRIMER_RIGHT_1_SEQUENCE=TGGAGCAACTTCGTATTAGCAA
PRIMER_LEFT_1=545,22
PRIMER_RIGHT_1=836,22
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=59.911
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.1188
PRIMER_LEFT_2_PENALTY=0.031769
PRIMER_RIGHT_2_PENALTY=0.087031
PRIMER_LEFT_2_SEQUENCE=GATAGGACTTCAGGTTCATGCC
PRIMER_RIGHT_2_SEQUENCE=ATGCTCAGTGCCCTTAGAAAAG
PRIMER_LEFT_2=399,22
PRIMER_RIGHT_2=673,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.1829
PRIMER_LEFT_3_PENALTY=0.095831
PRIMER_RIGHT_3_PENALTY=0.087031
PRIMER_LEFT_3_SEQUENCE=CTTCGTGAGCTCAAACTGACAC
PRIMER_RIGHT_3_SEQUENCE=ATGCTCAGTGCCCTTAGAAAAG
PRIMER_LEFT_3=503,22
PRIMER_RIGHT_3=673,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.2016
PRIMER_LEFT_4_PENALTY=0.021195
PRIMER_RIGHT_4_PENALTY=0.180440
PRIMER_LEFT_4_SEQUENCE=CTTGCAGAATCAAGAAGCCTTT
PRIMER_RIGHT_4_SEQUENCE=AACTTTGGTTGAGTTAGGCTCG
PRIMER_LEFT_4=545,22
PRIMER_RIGHT_4=811,22
PRIMER_LEFT_4_TM=60.021
PRIMER_RIGHT_4_TM=59.820
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_04318 Ps_contig_mira-and-tgicl-ass_franssen_32842
SEQUENCE=CGGGAAGGAAACTACGAATACATGATAGGCGATATCAGTTACCGGTTATCTATGTACACACTCTCCCCAACCAAATCTCTTNATTCACGCGCTTCTCACCCACTTTTTTCACTTCCAACTTTCGTCTTCGAGCTTCTTCTTCTTCATCTTCTTCAACCACATTTCTTGACACCAATCCAACACTGGATTCCGTTGTAGTTGAGAAGGAAGTTACTCGGAGCAGCAATCCCTTGGCTTGTCCCGTATGTTACAACTCTTTAAATTGGACTACTCATCATGCTTTGTCCATAGATACTATCCTTGGATCTAGTTTACAATGTAGAACCTGTCAGAAAGCATATGTTGGTAACCAAACGCATCTTGATCTCACGGCAATAAGCGGTGCTAAGAACTATGGTGAATCCATGCCAGCTTCCACTGAGCTTTTCAGGACGCCGTTGATATCATTTCTCTATGAGAGGGGCTGGCGTCAAACCTTTTCTGTATTGGGTGGTTTTCCAGGTCCCGAGAAAGAGTTTGAAATGATGAAGGGTTTCCTAAAACCAATATTGGGTGGAAATATCATTGATGCTAGTTGTGGAAGTGGGTTGTTTTCAAGATTATTTGCAAAAAGTGGACTTTTTTCTATGGTTGTGGCTCTGGACTACTCAGAGAACATGTTGAGGCAATGTTATGAGTTTGTTCAGCAGGAAGATAACTTTCCAAAAGAGAACTTCATCTTGGTTAGAGCAGACATAGCTAGACTTCCTTTTGTTTCAAGTTCCGTTGATGCTGTGCATGCAGGAGCAGCTCTCCATTGTTGGCCTTCACCATCATCAGCTGTAGCTGAAATAAGCCGTGTTTTACGGCCTGGAGGTGTCTTTGTTGCAACCACCTACATACTGGATGGCCCTTTTAGTGCTGTCCCATTTCTAACTACATTTCGCCGGAGTGTAAAGCAAGTATCTGGGAGCTGCATCTTTCTCTCTGAACGTGAACTTGAAGCTCTCTGCAAGGCGTGCGGACTGGTTGGATTTAAATGCATTAGAAATGGGCCTTTTGTGATGATTTCTGCTGCAAAGCCTAAATAAGCGGTTCGAAAAGCTGCACGCTGATCTGTTAAAATTGTGGTTTCACTGGTACAAAAAGATAGCTTCATTTAGAAAAATGATATTTAGACACACTTTTTCCTTAGATTATTCGCATATCATAAGTTGGGAAGATCAGAACTTCACACCAAAAAAAGTGTCGATTTCTAAAGAAGGTGTAAGGAGTGTCCATTCATCATGATGTGTGAGAAACGTGAAGCCAAATTTATTTGTAAATATTACTTCATTTGACCCTTCTACTTAATTGG
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0790
PRIMER_LEFT_PENALTY=0.060191
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=CTCCCCAACCAAATCTCTTNAT
PRIMER_RIGHT_SEQUENCE=TGCTCCGAGTAACTTCCTTCTC
PRIMER_LEFT=62,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.5500
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=0.0790
PRIMER_LEFT_1_PENALTY=0.060191
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=CTCCCCAACCAAATCTCTTNAT
PRIMER_RIGHT_1_SEQUENCE=GCTCCGAGTAACTTCCTTCTCA
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=220,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.5500
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.0969
PRIMER_LEFT_2_PENALTY=0.078029
PRIMER_RIGHT_2_PENALTY=0.018835
PRIMER_LEFT_2_SEQUENCE=ACTTCCAACTTTCGTCTTCGAG
PRIMER_RIGHT_2_SEQUENCE=GCTCCGAGTAACTTCCTTCTCA
PRIMER_LEFT_2=110,22
PRIMER_RIGHT_2=220,22
PRIMER_LEFT_2_TM=59.922
PRIMER_RIGHT_2_TM=60.019
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=0.0969
PRIMER_LEFT_3_PENALTY=0.078029
PRIMER_RIGHT_3_PENALTY=0.018835
PRIMER_LEFT_3_SEQUENCE=ACTTCCAACTTTCGTCTTCGAG
PRIMER_RIGHT_3_SEQUENCE=TGCTCCGAGTAACTTCCTTCTC
PRIMER_LEFT_3=110,22
PRIMER_RIGHT_3=221,22
PRIMER_LEFT_3_TM=59.922
PRIMER_RIGHT_3_TM=60.019
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=112
PRIMER_PAIR_PENALTY_4=0.1334
PRIMER_LEFT_4_PENALTY=0.078029
PRIMER_RIGHT_4_PENALTY=0.055411
PRIMER_LEFT_4_SEQUENCE=ACTTCCAACTTTCGTCTTCGAG
PRIMER_RIGHT_4_SEQUENCE=AGTTCTTAGCACCGCTTATTGC
PRIMER_LEFT_4=110,22
PRIMER_RIGHT_4=392,22
PRIMER_LEFT_4_TM=59.922
PRIMER_RIGHT_4_TM=59.945
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_04319 Ps_contig_mira-and-tgicl-ass_franssen_32843
SEQUENCE=GGGGGGNNACCCAAATATCAATATCATAACAAAATCTCTCTCTCGAATTACAGAACAAGTGTTGTGAGGGATCAGTGGATGGGGTGCTGCTAGTTCCTCACTTCATTCCAAAAATGGTGCACAGACTCTCTCTAATGGCTTCTCACGTCTACCCTTCACCCCTCGCCTTCCCCACATCACACATCAAGGACTGTCAAACTTTTGCACCTTCTTCTTTTGCAAAACCAGACATGCTCAATTATCAGTATCCTTTTCTTAAGGCGAGTCCATTTGTAGAGTCGATGAAAACCCAAAATAAATGGTTTGATGAAAGTCAGCTCGTCAATGTTGATTCCTCTGCTCAATGGCCGGTATCAATTGATGCACAAGAATCCTGCTCAAATGCAGTACTTTTAGGTTTTGGCATTGTTGAGCAATGCACTAAGCATGATACAGTTTCAAATCTCCTAAAATCGGGAACTGCTGAGCCGCGTACAGATGGTGCAAATATATCCTTGTTATTAGACTTGATGAAGTTGCAGTTGTCAGCCATTAATGAACCTCAACAACCATTCTCATCTGACTCGTCATCTCTTTTATACCCTAATGACAAATTCAACATCAAGAAGCCTTTGCTGTATTTTCTTCAAGATTCCGCACTTACTTCAAAGGTTACAGTTCATCTTGATGGCCAAATCACATTTATGGGTGCTGAGATTCAGATGAAAGATCTTCTTTCTGTAGTTGCTGAGTCATACTTGTCAAAGAGCTTACATAAGGGTGAAAAGCACTCAATGCTTGTTCCACACTTCAGCAGGGTGAATATCGATGAAGCAGAAGTACAGAGTCATTCATCTACAATGAAGATGAAATCTACCTTAACCGCACCTTTAAGGAGTCCTGAGAAAGTCAAACTCAAACCATCAAAAAAGAAGAATAAGAAAACCAACAGAGGAAGAGATATCTACAAGAACTATTCACACACATGTGAGAGCCTTCTGTCCTTGATGGTCAACAAGAAACAAAATCGGAAAACAATAATTCCTTCGTTGAAGAAATCCGGCCCCGAGCTTCCTGAGCTTCTTATGCAAATTTCTGCTGGGATTGCTGGAACTGGCCTTGCTGTCCTACTATCTGTTATGTGTAAGCTAGCTTGTGGAAGGGTTCTCCTTTCTGCATCCAGCTTTTTCAATACCGGGTTTGGGATTTGGGTTGGTTTGGCTTTCTTGGGCAGTAAGTAAGCTAAGGGCCACAATCTTTAGCATCAGCAAGAATGCGGTAAAATCGGGTTTGAAAGAAGAGGTAATGATTCAGAAACTCGATAAGAGGATTAGAGATATTTACTTCGGCTCCGCAGCATTACTAGCAGTGGCAGTGCTAAGTCTTGCTTGAGTAACATCTCCCAAGATGTAGTTTGTTGAACTGTAAATGTGAATGTCTATAGTGATGATTAAACAGTTCTAGTAAGAAATATTTGAAGGTATCAATTGATCAACAATCGTATGTAGTTAATGTGTCATAGTTTCAGGAAGCATTGTTTGGTATTGTAGTTTGTAATGTAGAATTTTGCTATGGAATCAAGCAATTGGCTTTCTCTAGTATAATAAAATTAGTACTTAGGTCAAGACTGAATTAG
TARGET=35,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04320 Ps_contig_mira-and-tgicl-ass_franssen_32847
SEQUENCE=CCACCGTATCTACCRCCAACTTCCTTCCTTCCGNACCATCCTCACCCTAGCAGCTGCTCTCTCAACCGAAGACAGAGAAGTCTTGGCTTACCTCATCTACTGTTCATCCGCTGCAACCCCTACCGGCAACCCAAAACGGAGCACCGTTAGGAACACCGATCATGTTACTCTCTTCAACTGTAGTTGTTTCCGATGCTACACGAGTTACTGGGTCAGATGGGACGAGTCGCCGAATCGTGAACTCATACATGAAATCATCGATGCTTTTGAGGAGTGGCTGGCTCAGAGTAATAAAGGTGAGAAGAAAGGGAAAGGAAAGAAAGAGAAGAGAAACAAGAAAGGGTTTGGGAATAAGCGCTATGGTCATACTCATTCCGGTGTGTTGAATCGGTCTTTGGTGAACGAGTCGACTCAGATGGAATCGGTGGGGGATAGTAGTAATAGTGTTATTAGTAGCGGTTGTGGTGGTAGTGAAAGTGGTGAAAGGGTGGTTGTTGTTGTTGGTGGTGATGAATATGAAGAGAAAGGGTCGGTGAGAAAATTTATGAGTTTCATTGGAGAAAGGATTTGGGGTGTTTGGGGATAGTGAATGAATTTGCAAGAGAAAAAATAATGAAGAAAATTTGAAGAAATTAAGGTAGGTCTAGCATATGTTTTTTCTTTTGTTGTTAAAATCTTCTTGTATATTAATCATCACTATGTAATGTTAAAAAAATGTTTATTTGGTTTTGTGATGAATGGATTAGATTTTGTTGTTTAACATATCATACCAGTGCTCTGGTTTATGTTTTCTTTTTGCTTCAATGACATNAYCTTGATTTTAG
TARGET=20,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04321 Ps_contig_mira-and-tgicl-ass_franssen_32847
SEQUENCE=CCACCGTATCTACCRCCAACTTCCTTCCTTCCGNACCATCCTCACCCTAGCAGCTGCTCTCTCAACCGAAGACAGAGAAGTCTTGGCTTACCTCATCTACTGTTCATCCGCTGCAACCCCTACCGGCAACCCAAAACGGAGCACCGTTAGGAACACCGATCATGTTACTCTCTTCAACTGTAGTTGTTTCCGATGCTACACGAGTTACTGGGTCAGATGGGACGAGTCGCCGAATCGTGAACTCATACATGAAATCATCGATGCTTTTGAGGAGTGGCTGGCTCAGAGTAATAAAGGTGAGAAGAAAGGGAAAGGAAAGAAAGAGAAGAGAAACAAGAAAGGGTTTGGGAATAAGCGCTATGGTCATACTCATTCCGGTGTGTTGAATCGGTCTTTGGTGAACGAGTCGACTCAGATGGAATCGGTGGGGGATAGTAGTAATAGTGTTATTAGTAGCGGTTGTGGTGGTAGTGAAAGTGGTGAAAGGGTGGTTGTTGTTGTTGGTGGTGATGAATATGAAGAGAAAGGGTCGGTGAGAAAATTTATGAGTTTCATTGGAGAAAGGATTTGGGGTGTTTGGGGATAGTGAATGAATTTGCAAGAGAAAAAATAATGAAGAAAATTTGAAGAAATTAAGGTAGGTCTAGCATATGTTTTTTCTTTTGTTGTTAAAATCTTCTTGTATATTAATCATCACTATGTAATGTTAAAAAAATGTTTATTTGGTTTTGTGATGAATGGATTAGATTTTGTTGTTTAACATATCATACCAGTGCTCTGGTTTATGTTTTCTTTTTGCTTCAATGACATNAYCTTGATTTTAG
TARGET=491,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04322 Ps_contig_mira-and-tgicl-ass_franssen_32850
SEQUENCE=TTCTGTTTAACCTAACTTAACCATCCTCCCTCTCTCCACCTNCCCTTTACTCAAAACCGCACTTTCAAACTCTCCCTTCCCCGTTATGCTCTCCATCAACCCAAACCCTTAACCGTCACTTTCGCCGCGCCGGAACACGACTCCGATCATGGTCAAGTTGAAGTGGAGAAGGGGAATGACGATGTTGATGTTGATGTTGGTTCCGAGGAGGAATCACAGGAAGCATGGAAGCAGGCTTTGGATACATTCAAGGAACAGGCTTTGAAGGTTCAAGGGGTTTCTCAAGAGGCTTATGAATTGTACTCGAAGAAGGCGGTCGTTATTTTGAAGGATACTTCGGAGCAGTTGAAGATACATGCTGATAAGGCCAAACATGATTTGAGTGTTGTGGCGAAGGAAATTACGGAAGAAGGGAAAGAGTATTTGTCTTCTGCTGCGGAGAATTCGCCTGAGGTGAAGGAGATTGTGGAGACTTTTACTTCCCCGGAGGATGATCTTAGCAATGTTTCTGGAGTGAGGGATTTTTATGTTGGGATACCCTATGGTTTGCTACTATCTCTGGGTGGCTTCCTTTCCTTTATGGTAACCGGAAGCCTTGCAGCAATAAGGTTTGGGGTGATTTTAGGCGGTGGTCTGTTGGCTCTAAGCATTTCAAGTTTGAAATCATATAAAAAAGGACAGCCTTCTTCTCTAGCTTTGAAGGGCCAGACAGCAATAGCAACTATCTTGTTTCTGCGGGAGATTAACTCAGTTGGCAGAGGATCAACTTATTTCACTGCCTTGATCAGTGGTGCGGTAGCGGCGTTTTACGTCTATAGACTGGTTTTGGAAGGTAAACCACAAAAAGGTTCAAACTTGGAAGGTGAAGCAGGAAACTAGTTCAGTGATAAGTGTCAATTGGAAGATCAGACAAAAAAACAGAAGATGCATGAAGACTGTCATAGTAAATTTTTGGTGATTTATTTATTTCTTTGATATTCTGTGGCCGGGTTGTCAAGACTCGTAGCGGAGGACTAGGGTTACGGTTTCATGCATGAAAAAATTGAGCATCTGGAAAATCTTGATATACTTTAGAAGTACTCTAGTGTATAATTATGATGATTTATGAATGAACTTTTCTTTTAGGTGGGATATTATGAACTGGTTTTATTTATTTTTTGAAAAACG
TARGET=958,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2239
PRIMER_LEFT_PENALTY=0.122257
PRIMER_RIGHT_PENALTY=0.101680
PRIMER_LEFT_SEQUENCE=GGTTTTGGAAGGTAAACCACAA
PRIMER_RIGHT_SEQUENCE=GCATGAAACCGTAACCCTAGTC
PRIMER_LEFT=821,22
PRIMER_RIGHT=1032,22
PRIMER_LEFT_TM=60.122
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.2263
PRIMER_LEFT_1_PENALTY=0.124655
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=TAACTCAGTTGGCAGAGGATCA
PRIMER_RIGHT_1_SEQUENCE=GCATGAAACCGTAACCCTAGTC
PRIMER_LEFT_1=743,22
PRIMER_RIGHT_1=1032,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=0.2320
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.101680
PRIMER_LEFT_2_SEQUENCE=GGGAGATTAACTCAGTTGGCAG
PRIMER_RIGHT_2_SEQUENCE=GCATGAAACCGTAACCCTAGTC
PRIMER_LEFT_2=736,22
PRIMER_RIGHT_2=1032,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=0.3239
PRIMER_LEFT_3_PENALTY=0.222249
PRIMER_RIGHT_3_PENALTY=0.101680
PRIMER_LEFT_3_SEQUENCE=CTGGTTTTGGAAGGTAAACCAC
PRIMER_RIGHT_3_SEQUENCE=GCATGAAACCGTAACCCTAGTC
PRIMER_LEFT_3=819,22
PRIMER_RIGHT_3=1032,22
PRIMER_LEFT_3_TM=59.778
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.3437
PRIMER_LEFT_4_PENALTY=0.242013
PRIMER_RIGHT_4_PENALTY=0.101680
PRIMER_LEFT_4_SEQUENCE=TGGAAGGTAAACCACAAAAAGG
PRIMER_RIGHT_4_SEQUENCE=GCATGAAACCGTAACCCTAGTC
PRIMER_LEFT_4=826,22
PRIMER_RIGHT_4=1032,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_04323 Ps_contig_mira-and-tgicl-ass_franssen_32853
SEQUENCE=GACATATCAAATGATTCTCCAATTGTCGGGCTTGCGAACGGTGAGATCGAGACACCGTTGTCGAGTGTTGCGAAACAGCGGGGAAGTCGGATGAAGAAGACTCCGGGTTCTGGTGAAGCTTTGTTGAGGGGTCAAGTGAAGACCCTTTTGCAGAAAGTTGAAGAAGAAGCTGAGATTTCAAAACTCACTATGGATACTCGTCCCTTTCCTCAGTTTGTTAACTCTCCAATGCATCTTCTTGCTCCAACTCCTGCAAATACCCCTCAAATCCCCAATATTAACAACAGTGTCACTGGGTTCACAGAGTTCTCACCTTCTTCTGTTGTTCAAGAACAACTCATTCCTCAGGTGGTGAATGAAGGAGCTGTTGGAAAGAATGAAGAGAAGAACATTGAATCTGAAAAGAGTGTGTTAAGTCCAGTGCTGTTAGATTTCTCAGACAAATCTGAGGTTACAGAAGATTCTAAGATGGGGTCGACAGAAGATGATGGTGATTCCATTTGGTCAATTCAAGTGAATGCAAGTACACATGATGATGAAGATGAAGATGAGGAAATAACCGAAGTTGAATATGATGATGTGGAAGATGACTACTATGAAGATGTTGATGAAGAGGAAGAGCATGATGATGGAGGGTTGTTGATTGATGAACTATGTGAGGGTTTGCGCAACATTGGTGTGAACGAAAAGGTTGTTCCAAAATTTGCTGGGAAACATACTAGGTTTGTGTATGGTAGTGATGATGATGAGATTGTGGATGAGGTTGTTGAAGAATCTGGTGGTTGTGATTCTGAGGTTTTGCATTTGAAAGGGTTGCCTACTCCAAAAGGAAAGCACATTCGTTTTGAGGAAGAGGAAGAAAACTGAGTTGTGAGTTGTGTTTTTTTAGTGTTTTGTTGTTGCTTGGTTGTCTCTCTTTTGACACCTTGTTTTTTGGTTTTTGTATTTCTTTTAATCTGTCTGAATGGAG
TARGET=738,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0531
PRIMER_LEFT_PENALTY=0.044832
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=TTGATGAAGAGGAAGAGCATGA
PRIMER_RIGHT_SEQUENCE=CAAAACCTCAGAATCACAACCA
PRIMER_LEFT=604,22
PRIMER_RIGHT=800,22
PRIMER_LEFT_TM=59.955
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=0.1989
PRIMER_LEFT_1_PENALTY=0.044832
PRIMER_RIGHT_1_PENALTY=0.154023
PRIMER_LEFT_1_SEQUENCE=TTGATGAAGAGGAAGAGCATGA
PRIMER_RIGHT_1_SEQUENCE=TGCAAAACCTCAGAATCACAAC
PRIMER_LEFT_1=604,22
PRIMER_RIGHT_1=802,22
PRIMER_LEFT_1_TM=59.955
PRIMER_RIGHT_1_TM=60.154
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=199
PRIMER_PAIR_PENALTY_2=0.3127
PRIMER_LEFT_2_PENALTY=0.044832
PRIMER_RIGHT_2_PENALTY=0.267873
PRIMER_LEFT_2_SEQUENCE=TTGATGAAGAGGAAGAGCATGA
PRIMER_RIGHT_2_SEQUENCE=AAACCTCAGAATCACAACCACC
PRIMER_LEFT_2=604,22
PRIMER_RIGHT_2=798,22
PRIMER_LEFT_2_TM=59.955
PRIMER_RIGHT_2_TM=60.268
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.3937
PRIMER_LEFT_3_PENALTY=0.385416
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=GGTCAATTCAAGTGAATGCAAG
PRIMER_RIGHT_3_SEQUENCE=CAAAACCTCAGAATCACAACCA
PRIMER_LEFT_3=502,22
PRIMER_RIGHT_3=800,22
PRIMER_LEFT_3_TM=59.615
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.4083
PRIMER_LEFT_4_PENALTY=0.044832
PRIMER_RIGHT_4_PENALTY=0.363436
PRIMER_LEFT_4_SEQUENCE=TTGATGAAGAGGAAGAGCATGA
PRIMER_RIGHT_4_SEQUENCE=GAATGTGCTTTCCTTTTGGAGT
PRIMER_LEFT_4=604,22
PRIMER_RIGHT_4=840,22
PRIMER_LEFT_4_TM=59.955
PRIMER_RIGHT_4_TM=59.637
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_04324 Ps_contig_mira-and-tgicl-ass_franssen_32855
SEQUENCE=TTGCGGGACAACTATTTTTCTCTTCTTTTTCTTTTTGAGAAATATTCAATTTCGTCCAATTCGTTTGCGAAACCCTAATTTCGATCCTTTTTTATCTCTCTCTACGACTCAATTTTCTCTCTCTAAACACTACAAATTTCGATCCCTCTCAGTCTCACTCTGATTCATCGCTCGAATTTCTCATCCACTACCAATTTCTCCGCCACACAACGAAAATCTCTAAAATCCTCAATAAGATCCACCGGTTAACTTCGATTTCGTTTTCATTTTTCCGATTAGGGTTTCGATTCAGGTCATTCAAGGCTGGCGAGTGTTCGGGTCAAGTAAATGGCGGATCCGTCTGGTATCCGCCATCATAATTGCTTCAATCACGGTGTTGCTTTAACAGTTACTGGAGCCATTGATTCTTGTGTTCGATTGTTGATTTGGTTGCAGGGTAGTGACGATTGCGAAAAGTGAATAATGCTGAACGTGCTACGTGTGCACCTTCCTTCTGATATTCCCATTGTAGGCTGTGAGCTCACGCCTTATGTGCTCCTACGTCGGCCTGACAAAACCGTTACGACTGACGATGTACTTGAAACCGCACCCTTAGATGGGCATTTTTTGCGATATAAGTGGTATCGTGTACAGAATGATAAAAAGGTTGCTGTTTGTAGTGTACATCCATCCGAGCAGGCCACACTGCAGTGCCTAGGTTGTCTTAAGGGTAAAATTCCTGTTTCCAAAAGTTACCATTGCTCTCCCAAGTGTTTCTCAGATGCATGGCAGCACCACCGAGCGTTACATGACCGAGCTGCAAGTGCAGTTAATGAAAATGGAAATGAGGAGGAGGAGGTATTTGGGAGATTTAACAATTCTGGATCTGGGTCAATCAATACCGGCTTATCCTCCTCTGCATCTAGTGCTAACCTGTCAAATGGGTCTGCAACTGTGTATCCCGCAACTGTTACACAACGAAATGGTGGTGAAACTTGGTTTGAAGTTGGACGATCAAAGACATATACCCCATCAGCTGATGACATTGGTCATGTTCTTAAATTTGAATGTGTTGTAGTTGACGCAGAGACAAAACTTACTGTGGGGCACACAAACACTATTCTAACTTCCCGGGTCATTCCTGCTCCTTCACCTATCCCGCGCCAGCTGATACCTGTTGATGGGATGGGGAGTTTGGATATGGATGGGCGTATAACATCATCGGGAACTTTTACTGTTTTATCATACAACATATTGTCTGATTCATATGCCTCAAATGATCTATACAATTACTGCCCTTCGTGGGCTCTTTCCTGGCCGTATCGCAGACAAAATTTATTACGAGAAATAGTTGGTTACCGTGCCGATATTATTTGTCTACAAGAGGTCCAAAGCGATCATTATGATGAATTTTTTGCTCCTGAACTGGACAAACATGGCTATTACGGTCTTTACAAAGAAAAAAACAAATGAGGTATATAATGGCAACATAAATACTATTGATGGTTGCGCAACATTTTTCCGTAGAGACAGATTCTCACATGTGAAGAAATACGAGGTTGAGTTTAATAAGGCCGCACAATCTTTAACAGATGCCATGATTCCAACCACTCAAAAGAAAACTGCATTGAACCGGCTTGTTAAGGATAATGTTGCACTAATAGTTGTTTTAGAAGCAAAAGTTAACAATCAGCCGGTTGATAATCCAGGGAAAAGACAGCTTCTGTGTGTGGCAAATACACATGTAAATGTCCACCAAGAACTAAAGGATGTTAAGCTCTGGCAGGTGCACACACTTTTAAAAGGATTGGAGAAAATTGCTATAAGTGCAGACATTCCAATGCTGGTTTGTGGTGACTTCAATTCAGTTCCAGGAAGTGCTCCTCATGCACTTCTTGCGATGGGGAAGGTAGACCCATCCCACCCAGATTTAGCAGTTGATCCACTCAACATATTGCGTCCTCACAGCAAATTGGTTCACCAGTTGCCACTGGTTAGTGCTTACTCTTCTTTTGCAAGAACGGTTGGCCTGGGATATGAACAGCATAAGCGAAGATTGGACAGCTCAACAAATGAACCTTTATTTACAAAGGTCACTAGAGATTTTATTGGCTCTCTTGATTATATATTTTACACAGCGGATTCATTAGTTGTGGAGTCATTATTAGAGCTATTGGATGAAGAGAGTTTGAGGAAAGACACAGCACTACCTTCTCCTGAATGGTCCTCAGATCATACAGCTCTCTTAGCTGAGTTTCGTTGCTGCAAAAGTATATCTAGGCGATGACTCATAAGTCTTAACTCATAAGGACAGATCCAGTGACACGGAAAGAAAAAAAGTTGAAAGAAGCATCTCGTTGATAAGTTATTGTTGTATAAGTAGTGGTAGATCATACCATTGTCTTTTTCTCAGGTATCTCCAATGTGGGGTTCCTTGCAATGATCTACTTTGTATTATGCCATCTTTATTTTATAGAAAATATGATAAGGCCTTACTGATATGCCTCTTTCATTCACCCTGTCCCTAATCTATTTATTAACTGAAAATTTTCTTCTTATNATTCCACCGGCTCAGTACATGTAGCATTGTG
TARGET=826,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0724
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.071684
PRIMER_LEFT_SEQUENCE=AAAGTTACCATTGCTCTCCCAA
PRIMER_RIGHT_SEQUENCE=ACCCATTTGACAGGTTAGCACT
PRIMER_LEFT=727,22
PRIMER_RIGHT=924,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.928
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=0.0724
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.071684
PRIMER_LEFT_1_SEQUENCE=AAAAGTTACCATTGCTCTCCCA
PRIMER_RIGHT_1_SEQUENCE=ACCCATTTGACAGGTTAGCACT
PRIMER_LEFT_1=726,22
PRIMER_RIGHT_1=924,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=199
PRIMER_PAIR_PENALTY_2=0.0724
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.071684
PRIMER_LEFT_2_SEQUENCE=CAAAAGTTACCATTGCTCTCCC
PRIMER_RIGHT_2_SEQUENCE=ACCCATTTGACAGGTTAGCACT
PRIMER_LEFT_2=725,22
PRIMER_RIGHT_2=924,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=59.928
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=0.0746
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.073900
PRIMER_LEFT_3_SEQUENCE=AAAGTTACCATTGCTCTCCCAA
PRIMER_RIGHT_3_SEQUENCE=TTGTGTAACAGTTGCGGGATAC
PRIMER_LEFT_3=727,22
PRIMER_RIGHT_3=956,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=59.926
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=5.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=0.0746
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.073900
PRIMER_LEFT_4_SEQUENCE=AAAGTTACCATTGCTCTCCCAA
PRIMER_RIGHT_4_SEQUENCE=GTTGTGTAACAGTTGCGGGATA
PRIMER_LEFT_4=727,22
PRIMER_RIGHT_4=957,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.926
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_04325 Ps_contig_mira-and-tgicl-ass_franssen_32861
SEQUENCE=AGATAYGAAAACCCAATGCATGTATGAACAAAATATACACTAGTAGTAAGTAGTAACATCATTGTTCAATACAGACCACTTTTGACAATTTTTTTTCATTATTCTATTTTGTGTTACCATATACACATGTAACTAATCTGTAACTAATATCATCAAGAATCAGATGAAGTTCAATTGACTGTACATTTATAGGATTTACCAGAAACTGTCGTCGCCGGATGATGTCTCATCATCTTCTTCCAACTCCATCTGAGGCTGTGGACTCCCGAATTCAGATGTTTCAATGTCTGCCAATTTCTTCAAATCAACCACTGAAAGCTCATCCAGGCGCCTCACAACCAAGTCTGCAGCCCTAAGCTCATACACGGGATGCTTGCTAGCAACAGCAACACACTTCATCTGTGCATCATGTGCAGCCTCCACTGTTAGATTTGAGTTTCCAAACACAATGCATCTTTCAGGTATGAAGTTCAAAAGCTGGGCTGCATACGCAAACATTTCTGGATCAGGCTTTCCCCTGTGAACATCCTCGGCTGCAACAATAACATTGAAGTTTCCTACAATACCAATTTCCCCCATAGCAGACTCGATAACTTTTCTAGGCCGTGTAGAAACCAAAGCCATCGGTATCTTATAATGCATCAAAACACCGACAAACTCTTTCGACCCAGGCATGAAACTATATATCCCACCTTGTAAAGCTTGGTAGATTTCTTCCTTTCTATTCGCCATTCTTCTCAGCTCAGTCCGATCTCTAGACCAACACAGCACTTCGGAAATTGCCTGCTCACTCTTCATACCCTCAATTCTCTTGATCACAAAAGCCATAGGAGACGGTTTCCCCTCCTCTTGCGCAAGCGCAAGCCAAGCTTGCTTCTCTAAATCAGGGTTGTCTTCAATAAGAACCCCCTCCCACTCAAATATAGCCCCCAACCAACCACATCCCATTCTCTCTTGTCGAAGCAAAGGGTTTCTCAGCGAAGGCTTATCAGCTCGATTCTCCGGTGGCCACAAATCAGGTCTCCGATCAAAACCACCATCAATCTTACTATTCCCTTTCTTCGGCAATCTGTCCTCCCTATAAGCATATGCTTCTCTCGTAAGCTCCACCGCAAGAGCCTTGATCGACGAAGACATCAAGAACCCATCTTGCTTAGATTTAATCAAAGGCAGTGAAACAACAACTTTTCCACCAAGAAACTCAGCTGGTGGAAACCTGCAAGTGTTGAAAGATTTCCTCTTTCCAGAAACAACCCTAATCAAATTTCCACCGCAAACCGGACGGTAGCCAAGGATTGAAGTTGCAGCAATTGAATCAACCATCTTAACAACAAATAAACCTCAAACCCTAACCAAATTGAATCAAAAATCAAATTTTGATTACAATACAAAAGACCCTTCAAGGGTCACAGTGAATTCCACAAAAGGAAAAACCAATTTCACTAAGAAGAAACCTATTAAACGAAAATCCCAACACCAAATTTGAACCGCGATTCCTGTCCAGAAACTATATTCTTGAAAACGACACCATCTCGGAAGCAAAACGACAACGACAAACAGAGTTTTACCACAAAATCGCAACTTTATCGTCAAATAAATCCCCTGAAAGTGAAAGAGGATCAGAAACTTGAAGAGATGAATCGATTCTCTCTTCTTTAATGGAGAAGCTTTTCTTAATACGTTTCTTTGTTTATATTTTATTATTATTAGTTTGATTTATTTTTTAAAGC
TARGET=1698,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04326 Ps_contig_mira-and-tgicl-ass_franssen_32863
SEQUENCE=AGGGTTTTCGCAAGAGGAAGAANCAAAAAAAACCTAACACATTCTTTCCATTTTTTGCTTAAACAGTGCTGCGAAACCCCCCTCACTTTCTCACCGTAACATAGAACGCACAGAACAATGGGAGGAGAAGAAAAACGGCACCAGATGATGCAGAACCTCTTCGGAGATGAATCCGGTGAGGAAGAAGAACTCGAGTCCGAGCATGAATCCAATCCTCAACTCAATTCCGAGGATGAAGGAGAGGGAGAGGTAGGGCAAGAAGAAGGAGAAGGGGAAGTGGAAGGACAGGGAGAGGTGGAATTAGAGAGTGAAGGAGAGAGGGAGCCGGTTTCTCAAGAGATTGAAGTGGAGGTCGGTGATCAGAGAGAAGAAGAGAGTGGAGCTAGGGATTCGGATAGTGATGCAAAAGATGATTATAGTCAGCGTGTTGTTACCAGCAGGCGTAGGGATGTTGTTGAAAGTGGATCTGAGGAAAATCATTTCAATGAAAATGATGGTGAAGAAGTGGATGCAGCTAGAAGCCTGAGCGGGTCACCTAGAGATGACAATGATCAAACTCGTGATTTGCATTCTGCCCCTGAAATCCGTGATGTATTTGGTGATTTTGACGATGAAGAGGAGGACATGGGGTATGCAGCTCAGCAGGACATTGGACAAGATTCAAATAGATATCCAGCGGATGAAGAAGGGAGTTATGGAAAGAGCCTGAGACCAGAGGATATACTTGCTGATGAAGATCATCAATATGAATCAGAGGAGGAAAACATTGAAATGAAAACTAAAGATAAGCCACTTGGGCCCCCCTTGGAGTTAGAGATTCCATTACGTAAACCACCGGCTTCTCCTAATAAGATGAACTTGATTAAAGTTTCCAATATCATGGGTGTTGATCCAAAACCTTTTGATCCTAAAACATATGAGGAAGAGGATACCTTTGTAACTGATGAACTTGGAAACAAGAAGCGCATACGCTTGGAGAACAATATTGTACGTTGGAGAGAAATCAAAAATCCTGATGGCACAACTTCTGTTGAAAGCAACGCTCGATTTGTAAGGTGGTCTGATGGCAGTCTTCAGTTGTTAATTGGGAATGAAGTTCTTGACATATCAGAGCAGGATGCCCAACATGATCAATCACATCTTTTCCTTAGACATGGAAAGGGAATCCTGCAATCACAAGGAAGGCTATTGAAGAAAATGAGATTTATGCCATCTTCCTTGTCATCAAATTCTCATCGGTTGTTGACTGCTATTGTTGACTCAAGGCAAAGGAAGGTTTATAAGGTTAAAAACTGTGTTACAGACATTGATCCTGAGAGGGAAAAAGAGGAAAAGGAAAAGGCTGAAAGTCAAAATATTAGGGCTAGCGTACTGCTTAGCCGTAAGCGTGAGAAGGTGAATAAAAAATATACTCCAACTGACAGGAGGCGTCAACTTTCTCCTGGGTTTTTAGAGGATGCTTTGGATGAGGATGATGAAGCAGATTATCACGACTCTCGTCGTAAACAGCGTCACTTTGATGACGAATTGGAAGCAGNRGTYCRACGCAGAGAAAAGGATTATGAATGCTAAGAAGTCCCAGGGACCAAGGGACATCCCTCGTAAGTCTTCTTTCCCACCAGCAAGATCCTCACGCTACCCAGCTGACGAAAGAGAGGAATCTGAATATGAAACTGATGAAGATGAAGAAGAAGAAATACCTCATTCACGTAAGAGGGATGATGATACTGAGCCGGAGTACGAGGACTCCGAAGAAGATGATGAAGAAACCGCTCAAGCTGATGCTGCTGCTTCAGACGAGGAAGAAGAGGAGGAACCGAAGCATAAGAGTCGGGATTTAAAAGGTAAGGTCAAAAGGAAGGGATTTGAATCTGAAGAACACTCTCTTCCAAGAAAACCAACCAATCGGCGAATGACCATTGTGTCTGACAGTGACGACGAATGAAATTCTGTTCTTGCAATTGGAAGGAGCAATGTTTTTTGCTGCAGCACAAGCTGAGATGACTCATTTTGTTTTTACTCTCCTCTATATTCTTGCTTATACAAACCTAAAATTTAAAGTTATAGACACACCAAGAAGACACACAGGCACACATTAATTGGTAATTTGTCTCAGGTCTCTGTTGTATGTTTATTTCCAAAAACCATGGTCGAATCATCTCCATAAAGTTACAAAGCTATATGGAAAGTGCTAGGGTGATCAAATCCAAAATAAAAAAACTGCAAACTGAATAAACCTAAAAAACCAAAAACCTAAAA
TARGET=1685,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04327 Ps_contig_mira-and-tgicl-ass_franssen_32867
SEQUENCE=CAACGCAGAAAGCAAATCCATCCCCGCGTCCTGGCCCTTCCTCTCCCAGCTCTGTGTTCTACCCTTTTTTTCTTTCTTTTTTTCCTTTTTAACCATTTTTATTACTATAACATATACACCTTTCTTTCATAGGTTTCAAATCCTTTCTACCAATTTGCTTCTTTTTATCCAAAATTTCCCAGAGCTTCGAGGGTTAGGAGGTGTGGATTCACCGTTTCCCACCTGTGTTACTTTCATAACACCGCCGTTACCGTTTCGATCAAATTTTCTTCCGATAGATTTAGGGTTTCTCCTATATTTCATACTTTCTTTCTTTATCAATACTGTGATTTACGTTTTAAGAACCACCAATATAGCGTAACGATTTTGTCAGATTTAAGAAATGGAAAAGAAGAAAGCGACGGTGCCTCTTGTTTGTCACGGTCACTCTCGTCCAGTTGTTGATCTCTTTTACAGTCCTGTTACTCCCGATGGTTTCTTTCTTATTAGTGCAAGCAAAGATGCTAGCCCTATGCTGAGGAACGGAGAGACTGGAGATTGGATTGGAACATTTGAAGGGCACAAAGGTGCAGTTTGGAGTTGCTGTTTGGATGCTAATGCTTTGCGGGCTGCAACTGCTTCTGCTGATTTCTCAACCAAAGTTTGGGACGCATTAACTGGAGATGAGTTACATTCTTTTGAGCATAAGCACATTGTTAGAGCATGTGCTTTTTCTGAGGATACTCACCTTTTGCTCACTGGAGGAGCCGAGAAAATTCTCCGAATTTATGATTTAAATCGACCTGATGCACCACCCAAAGAAGTGGATAAATCCCCTGGTTCAGTCAGGAGTGTTGCATGGCTTCATAGTGACCAGACAATTTTGAGTTCATGCACTGATATGGGAGGTGTCAGGCTGTGGGATGTAAGAACTGGTAATATAGTTCAAACACTTGACACCAAGTCACCTATGACTAGTGCTGAAGTGAGTCAGGATGGTCGTTATATTACAACAGCTGATGGTTCTACTGTCAAGTTTTGGGATGCAAATCACTATGGATTGGTGAAGAGCTATGATATGCCGTGCACTGTGGAATCAGCTTCATTGGAGCCCAAGTTTGGGAACAAGTTTGTTGCTGGGGGAGAAGACTTGTGGGTTCATGTTTTTGATTTCCATACTGGCAATGAGATTGCATGCAACAAAGGCCATCATGGTCCTGTACACTGTGTCCGTTTCTCTCCTGGAGGAGAATCATATGCATCAGGATCGGAGGATGGAACCATCAGAATATGGCAAACAGGCCCATTGACTCCTGGTGAACCAGAGGGTTTATCACCAAATGGATCCCTAGAAAAGGAGAAGGCAAATGCAGAGGAGGATGTTTCACGCAGAATTGGTAGGGTTTTCATATTGCAGAGTGACGGGACCGAAACGTACCGGAAAAGGGGGAAAAAGGGAAAGGAA
TARGET=305,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4797
PRIMER_LEFT_PENALTY=0.307443
PRIMER_RIGHT_PENALTY=0.172268
PRIMER_LEFT_SEQUENCE=CGTTTCCCACCTGTGTTACTTT
PRIMER_RIGHT_SEQUENCE=GCTAGCATCTTTGCTTGCACTA
PRIMER_LEFT=212,22
PRIMER_RIGHT=507,22
PRIMER_LEFT_TM=60.307
PRIMER_RIGHT_TM=59.828
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=0.4981
PRIMER_LEFT_1_PENALTY=0.307443
PRIMER_RIGHT_1_PENALTY=0.190632
PRIMER_LEFT_1_SEQUENCE=CGTTTCCCACCTGTGTTACTTT
PRIMER_RIGHT_1_SEQUENCE=GAGTGACCGTGACAAACAAGAG
PRIMER_LEFT_1=212,22
PRIMER_RIGHT_1=428,22
PRIMER_LEFT_1_TM=60.307
PRIMER_RIGHT_1_TM=59.809
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.4981
PRIMER_LEFT_2_PENALTY=0.307443
PRIMER_RIGHT_2_PENALTY=0.190632
PRIMER_LEFT_2_SEQUENCE=CGTTTCCCACCTGTGTTACTTT
PRIMER_RIGHT_2_SEQUENCE=AGAGTGACCGTGACAAACAAGA
PRIMER_LEFT_2=212,22
PRIMER_RIGHT_2=429,22
PRIMER_LEFT_2_TM=60.307
PRIMER_RIGHT_2_TM=59.809
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=0.4981
PRIMER_LEFT_3_PENALTY=0.307443
PRIMER_RIGHT_3_PENALTY=0.190632
PRIMER_LEFT_3_SEQUENCE=CGTTTCCCACCTGTGTTACTTT
PRIMER_RIGHT_3_SEQUENCE=GAGAGTGACCGTGACAAACAAG
PRIMER_LEFT_3=212,22
PRIMER_RIGHT_3=430,22
PRIMER_LEFT_3_TM=60.307
PRIMER_RIGHT_3_TM=59.809
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=219
PRIMER_PAIR_PENALTY_4=0.5387
PRIMER_LEFT_4_PENALTY=0.307443
PRIMER_RIGHT_4_PENALTY=0.231272
PRIMER_LEFT_4_SEQUENCE=CGTTTCCCACCTGTGTTACTTT
PRIMER_RIGHT_4_SEQUENCE=GATCAACAACTGGACGAGAGTG
PRIMER_LEFT_4=212,22
PRIMER_RIGHT_4=445,22
PRIMER_LEFT_4_TM=60.307
PRIMER_RIGHT_4_TM=59.769
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_04328 Ps_contig_mira-and-tgicl-ass_franssen_32872
SEQUENCE=CAACAATAGTACTGACGATGAAGATGAACAAATTAGTCAATTGGATTATTGTTTAGATAATCAATCTAGTGGTGGTGGTGATCATGACATGATGGGACTTGGTCCATTACTTCCTACTGAATCCGAAAGGTCACTCATGGAAAGAGTTCGTCAAGAACTCAAAATTGAACTCAAACAGGGTTTTAAGTCTCGAATTGAAGATGTAAGAGAGGAAATATTAAGGAAAAGAAGAGCTGGAAAATTACCAGGTGACACAACTTCTGTCTTGAAGAATTGGTGGCAACAACATGCAAAGTGGCCTTATCCAACTGAAGATGATAAAGCAAAACTTGTGGAGGAAACAGGATTGCAACTGAAGCAAATAAACAATTGGTTTATAAACCAAAGGAAACGAAATTGGCATAGCAATTCTCAATCTGTTACCTCTTTGAAATCCAAACGCAAGAGGTAGTAATAGTGTTACTAATTACAACAAGTAAAACACAATGATAATATGAACTAATTACTTTATGGTTATTGATGATAGAGTACTATAGACCCTCCATTGAGATGGATCAGGAGATAATAGTAGTTATTTTGGTTAATTGGTTTACTGAAAAAATGTACTTATGATGTAGCTTAATTTCATAAGAGTTGTGTGGTTGTAAATTTGTGGAATTACATGTATGACTTTTTTTGCATGTGGTAAGGTTGTTTTGTCTCTTTCTTATTGTCTTTGTAAATTGTTTGTTACGTTCTGACCAGCTCATGGATTATTACTATGATACACACCACAACTTTGAAAGATTCACGTTTCTTTAATANACG
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04329 Ps_contig_mira-and-tgicl-ass_franssen_32882
SEQUENCE=AAGTAATTAAATAAATAAAAATTAGAAAATAAATAACCGCTAACAACATTCATCCTTCACACAGTTCTAAATAAAAACTCGGAGAGAGCCACTGCCGGTACCATCACCGTCTTTCAGTTTTCCGGCGATCTCTTCGTTTTCCACCGCCGTTCCCATATCGTACCAATCAAACATGACTACATCAAGGCGCCTCTCGGTTAGGAAGGTGGAGAAGTTTGAGAAAAACATTACGAAAAGAGGATTTGTGCCAGAAACAACTGTTAAGAAAGGAAAAGACTACCCTGTTGGTCCTTTGGTGCTTGGGTTCTTCGTCTTTGTTGTCATTGGATCATCTCTCTTTCAGATAATCAGGACAGCAACCAGTGGAGGCATATGATGGAGCTTTGAGTTCGACTGTATCATGTTTTCTTTCTTAGCCCTGTAGAAAATGTGACTATACTTTTCTATATTTTCTTGAACAATGCTCGGTTCTTGTTCATGTTTTGATGAAGTTAAGGATATTTTATTATAGATCCATTGCACCTCTTCTCTTTAATTTTACTTATTGTGGCGGATTGGTTTTAGTGGTTAGTTCCGAAAGCTATGAATTATGAATGTGAGTCTGACAGCATGAACTCATAATTCCTACACCCCAAATTCAATGGCAAGGGTGTGGACGTTTTTACTTTTGAACTTCAAGTAATTCACTTAA
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04330 Ps_contig_mira-and-tgicl-ass_franssen_32888
SEQUENCE=ATTTTGATTGTAARNAAACAATNAATCACAATCTCCGAGTCCAAGAAAAGAATCAGTCTGGATCTGACAGTAATACATAATCATCATCATCATCTTGCGCGAAGCAACGCAGCGAGAGAGTAAACTGGAAAGGATAGGTTAGTTAGGGATCATCGATGATACTCCTGCAGTCCCATTCAAGATTCCTGCTTCAAACTTTGTTGAATCGCCTTCAAAATATTGAAAAAGGTGTTGAACTCGATCATCACTGGGTTGAATTTGATGATGTTCGATATCATATCCAGGTGTCAATGAAGAATCCTCATATTTTCTTGCTATCAGTATCGTTGCCTACTCCATCTTCAGAAACTATNTTTGTATCTGGACTACCTTTTGGAGCCATTGAAGCAATAAAAGCTGCATATGGTAGTCTTGTGCAAATTCTTGATCCTCCCAGGGATGGCTTTAACCTCACGTTGAAGATAAATCTGTCAAAGGTTCCTGCAAATCAAGAGCAAAGACAAGCACTTTTAGTTAAAGTTGCATCAATAAGGGAGGTTGTTCTTGGTGCACCACTAAGAGTAATTTTAAAACACCTTGCTTCAAGAACTGTTGCTCCGGATATGGATCCACTTGTCGCTCTTGTTCATCGGCCAAAGGAGTCTTTTTTTGTTTTTCCTCAGGCTGATAAGGT
TARGET=80,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04331 Ps_contig_mira-and-tgicl-ass_franssen_32890
SEQUENCE=CAGAGTACGCGGGGATANTCRNNGGATNGACTTCTTATCTACTACACTCCACACTTCTTCTTCTTTGAACTCGCAGCACCAATGGCCACTTTAGAATCAGCCATAACCGTTTTCGCACCTCACCGTTTTTCAAACAACTACCGTTTCCTCGCTAAATCACCTAATTCCGTTAAACTCCAAGTCACTTCTATCTCTTCTTCCATTTTCTCCCATAGCCACAACAAACTTCACTTATCTTCTTTCAGAACCCAAACACCATGCTTCGCTTTACAAGAGGTAACAGAAGCAACAATTGAAGATGAAAAAACAGAGGAAACGGAGACATTAAACTACTTTAAGAACAAGTTTGCTGTGTTTAACTTACCTTGGTCACTGTCCGCGCAAGACATCAAAGAACTCTTCGCGGGATGCGGAACCGTAACCAGCATCAAGATTATAAAAGGAAAAAATGGGAGGGGAAAAGGTTATGCATTTCTTACTATGGATTCTGAAGAAGGGGCTCAGGCTGCTGTTGAACAATTTAATGGTCGTGAAATATCAGGCCGGATAATAAGGGTAGAGTTTGTAAAGATGTTCAAGAAACCTTCTCCTCCAGTCCCTCCTCCAAGTCCGGCTCCTAGGGAAGCAGCTCATGTAGTTTATGTATCCAACCTTGCGTGGAAGGCAAGACCTACACATCTTAGAGACATCTTCACTGAGAATTTCAAAGCGCCAGTTTCAGCCAGGGTTGTCTTTCAAACCTCTGCTGGGGGATCTGCTGGGTATGGATTTGTTTCTTATCATACAGAGGAGGAAGTACAGGCTGCCATTTCAGCTTTGGAAGGGAAGGAGTTACAGGGACGACCACTTCGCTTAAGAATAAGTAAAAAGAAAGTTAAAGCTGAGAATGAAGGTGAAAAACAAGATGCCGATGCTCAACTGGAAGGTGAAAACCAAAATGCCGATGCTCAACTAGAAGGTGAAAACCAAGATGCTGATACTCAACTGGAAGAATCATAAATACTAATTTTTCATGTACTAATTTTTGGACATGGTTACGGTATGTGGATCGAGAAGTAACCTCTTCATGCTGGGATTTTACCTCTGCATGTATACTACACAAAGGACGATTTCTTGCATCCACATTCTTTTTGAACTAGGTTGAGTTTGACACATCTTTAAATTATGAAATCACTTTCAATATAGTTTTCCGAATAAATCTCAAGTCTGTATTTTTTCCCTTTTCGCCTTACCCCTTAAAGTTTTTTTAAACCGGGTTTTTTTAGGTTTAAACGGTTTAATTGGTAATTTAGGTTTTAAAAAAAGG
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04332 Ps_contig_mira-and-tgicl-ass_franssen_32894
SEQUENCE=TCTATCTCACTCCTTAAGCCTATAGTGTGATAGAAAATCAAACAACACAAACAAACAAAGAAACTCAACGCACGCAGACTTCAAAATGCCTCTTCCTTCATCACAATGAAGAAACCTTTTCTCTTCATCTTCTCCATAGAAGATGTCTCTCTCACACCAACAATCTCCTTCCTTCCTCGACGCCCTTCTCTGTGAAGAGCAAAACTTTGAAGATGATGATTCCGATGCAACCGTCACTGATTCTGAAACCGTTTTTGATGACCCATCTGGGGCAAAGCTGCAGTCTTTGCCTTTGGTTTTGCTTAGCAATGATGTGTTTTGGGAAAGCGATGAGCTTGTTTCGTTGATTTCTAAAGAGGGTGAGACCCGTTTGTGTTGTGGGAATCTTGTTGGGGATGGATCTTTGGAAGAGATTCGTGTGGAGGCTGTCAACTGGATTTCTAAAGTTTGTGCTCATTATGGGTTTTCTACTTTGACTACTGTTCTTGCTGTTAACTACTTTGATAGATTTATTACTAGTTTGAAGTTTCAGAAGGATAAACCATGGATGACTCAGCTTACTGCTGTTGCTTGTTTGTCTCTTGCTGCTAAAATGGAGGAAACCCATGTGCCACTTCTTTTAGATTTTCAAGTTGAGGAATCTAGATTTGTGTTTGAAGCTAAGACTATACAGAGAATGGAGCTTTTGGTGTTGTCTACTTTGAAATGGAGGATGCATCCGGTGACACCAATTTCCTTCTTTGAACACATTGTTAGAAGGCTTGGTTTGAAGAGCCGTTTGCACTGGGAGTTTATGTGGCGTTGTGAGAGGGTTCTTCTTCATGTCATTGCTGATTCAAAAGTTATGATGAGTTATCTTCCTTCTACATTGGCCGCTGCTACAATGATCCATGTAATTAAAGAGATTGAACCATTTAATGCAACTGAGTACATTAATCAGCTATTGGGTTTACTAAAGATTAGTGAGGAACAAGTGAACCAATGCTACAAGCTTATGCTGAAGCTATTAGTTTCCGATGATGGCATTTATAGTCTTCACCAGAAACGGAAGCGAGTATCTGAACCAAGTAGCCCGGGTGGTGTCATTGACGCTTCTTTCAGCTGTGATAGCTCAAATGATTCATGGACCGTGGCATCGTCTGTTTCTCTTTCAGTGCAGCCGATGTTTAAGAGGAGCAGAGCTCAGGACCAGCAGATGCGACTTCCTTCTGTGAATCGTGTGTCTATCGATGTCCTTAATAGCCCTCGTTAATAATGAACGATCTTAGTATTTTTACTTCAAATGTGGTAGCTCTCTTTCACATAATTCTGGTGGCAGTTATGTTATTGATTCCAATTACATTATGTTCTAATTACTATATACATTTCTAGCCAAACATTTGAAAATGAATGGAATGAGGCAGAAGCTGAGATTCCTTCATCCACTTTGTCTATTAAGGTGGAGCAGGGATCTGATACTGAGTAGAAAGATGAGTTATGAACTTGTAATTCTACCTGTGAGTTATTTATCAACTTCAACTTTTACTCG
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04333 Ps_contig_mira-and-tgicl-ass_franssen_32894
SEQUENCE=TCTATCTCACTCCTTAAGCCTATAGTGTGATAGAAAATCAAACAACACAAACAAACAAAGAAACTCAACGCACGCAGACTTCAAAATGCCTCTTCCTTCATCACAATGAAGAAACCTTTTCTCTTCATCTTCTCCATAGAAGATGTCTCTCTCACACCAACAATCTCCTTCCTTCCTCGACGCCCTTCTCTGTGAAGAGCAAAACTTTGAAGATGATGATTCCGATGCAACCGTCACTGATTCTGAAACCGTTTTTGATGACCCATCTGGGGCAAAGCTGCAGTCTTTGCCTTTGGTTTTGCTTAGCAATGATGTGTTTTGGGAAAGCGATGAGCTTGTTTCGTTGATTTCTAAAGAGGGTGAGACCCGTTTGTGTTGTGGGAATCTTGTTGGGGATGGATCTTTGGAAGAGATTCGTGTGGAGGCTGTCAACTGGATTTCTAAAGTTTGTGCTCATTATGGGTTTTCTACTTTGACTACTGTTCTTGCTGTTAACTACTTTGATAGATTTATTACTAGTTTGAAGTTTCAGAAGGATAAACCATGGATGACTCAGCTTACTGCTGTTGCTTGTTTGTCTCTTGCTGCTAAAATGGAGGAAACCCATGTGCCACTTCTTTTAGATTTTCAAGTTGAGGAATCTAGATTTGTGTTTGAAGCTAAGACTATACAGAGAATGGAGCTTTTGGTGTTGTCTACTTTGAAATGGAGGATGCATCCGGTGACACCAATTTCCTTCTTTGAACACATTGTTAGAAGGCTTGGTTTGAAGAGCCGTTTGCACTGGGAGTTTATGTGGCGTTGTGAGAGGGTTCTTCTTCATGTCATTGCTGATTCAAAAGTTATGATGAGTTATCTTCCTTCTACATTGGCCGCTGCTACAATGATCCATGTAATTAAAGAGATTGAACCATTTAATGCAACTGAGTACATTAATCAGCTATTGGGTTTACTAAAGATTAGTGAGGAACAAGTGAACCAATGCTACAAGCTTATGCTGAAGCTATTAGTTTCCGATGATGGCATTTATAGTCTTCACCAGAAACGGAAGCGAGTATCTGAACCAAGTAGCCCGGGTGGTGTCATTGACGCTTCTTTCAGCTGTGATAGCTCAAATGATTCATGGACCGTGGCATCGTCTGTTTCTCTTTCAGTGCAGCCGATGTTTAAGAGGAGCAGAGCTCAGGACCAGCAGATGCGACTTCCTTCTGTGAATCGTGTGTCTATCGATGTCCTTAATAGCCCTCGTTAATAATGAACGATCTTAGTATTTTTACTTCAAATGTGGTAGCTCTCTTTCACATAATTCTGGTGGCAGTTATGTTATTGATTCCAATTACATTATGTTCTAATTACTATATACATTTCTAGCCAAACATTTGAAAATGAATGGAATGAGGCAGAAGCTGAGATTCCTTCATCCACTTTGTCTATTAAGGTGGAGCAGGGATCTGATACTGAGTAGAAAGATGAGTTATGAACTTGTAATTCTACCTGTGAGTTATTTATCAACTTCAACTTTTACTCG
TARGET=166,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3071
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.275288
PRIMER_LEFT_SEQUENCE=ATGCCTCTTCCTTCATCACAAT
PRIMER_RIGHT_SEQUENCE=CTAAGCAAAACCAAAGGCAAAG
PRIMER_LEFT=85,22
PRIMER_RIGHT=305,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.275
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.3930
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.361206
PRIMER_LEFT_1_SEQUENCE=ATGCCTCTTCCTTCATCACAAT
PRIMER_RIGHT_1_SEQUENCE=GTTTCAGAATCAGTGACGGTTG
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=59.639
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.4347
PRIMER_LEFT_2_PENALTY=0.031769
PRIMER_RIGHT_2_PENALTY=0.402961
PRIMER_LEFT_2_SEQUENCE=ATGCCTCTTCCTTCATCACAAT
PRIMER_RIGHT_2_SEQUENCE=GGTTTCAGAATCAGTGACGGTT
PRIMER_LEFT_2=85,22
PRIMER_RIGHT_2=249,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=60.403
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.4469
PRIMER_LEFT_3_PENALTY=0.171564
PRIMER_RIGHT_3_PENALTY=0.275288
PRIMER_LEFT_3_SEQUENCE=CAAACAAAGAAACTCAACGCAC
PRIMER_RIGHT_3_SEQUENCE=CTAAGCAAAACCAAAGGCAAAG
PRIMER_LEFT_3=51,22
PRIMER_RIGHT_3=305,22
PRIMER_LEFT_3_TM=59.828
PRIMER_RIGHT_3_TM=60.275
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.5168
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=0.275288
PRIMER_LEFT_4_SEQUENCE=CAGACTTCAAAATGCCTCTTCC
PRIMER_RIGHT_4_SEQUENCE=CTAAGCAAAACCAAAGGCAAAG
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=305,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=60.275
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_04334 Ps_contig_mira-and-tgicl-ass_franssen_32895
SEQUENCE=CYAATGAAAAAAGGTAGCAGTTCATATATGATCCTTTGAGAACATACAAGATCTTTGCCTTTTGTTAAAACCTTTCTAAGGTTGAGTAAAAAATAATAGTAGTTAAAAGGGAAAATTTGATACAACAAAGAAAGTACTCAATTTCTCACATGTTTTTAGTGATGTATACATTGATGAGCACTGAGTAGAAACCAGTAGTGGACTAACAAATTTATTTATTTTTCATTTTAGAACAGCTTTGATGATATTGATCTACCACTTGAGCCTTCAGTGAGCAAGACCTCATTTGCTCCATGAAACACCTAAACTCTGAGATGACATGTGGTGCCAGAGCTAATGAAACACCTCTCCAACAGACATTCTTTCCCAATAGAAGTTCAAGACGACACTGCATCTTTTTCCCCATTGCTGTTAAGTGCTTCTACCGGCACAGAAAACTGTGGCTCCAACTCAAGAGCCTCCTTGTACGCTTTCTTAGCTTCCTCCTCCATTTTCTTCCCTTCATAGCACTCCCCTAACGCACACCAAGCCTTAGCGTTCTTCGGACTCAAAATCACCGACTCAGTCAAATCCGCAAGCGCCGAGTCAACTCGTCCGCGTTCACTCGTCGCGAGTTTCAGCTCAGCTCTCTTAAACAACGCGTCACCACGTTCTCCCTCAGCAAGAGAACCAGCAGCGAGAGGAGACAACGCCGCGTCGAGCGATTCAAGAGCGGAGGTTCTAAAACCCTGAAGATCTAGAACCATGGCTTTGAGAAGATACGAAGCGGCGTCTTTAGGATCGAGTTCGATTGCTTTTTCGGCCTCGGCTAGGGCTTCGTTGGCGAGGTTGTTGATGGCGGATCTGGATTTGTTTTTGGAAGATCTCGCTTGTGTGAGAAGCTGAGCGCCTCTAACGAAGTGACGCTTGGCTTTGATACCGGTTCGGTTGCGGATTCGGAGTTTAGCGAGAAATTTCTGGGGAATACCGTGCATGCTGAGGAAGATTAAGATCATTGCAAGTATCATAACTATTTGTAAACCGATGTCGCGGATCATCGCGTGTTTCTCTTTGGTGTCCATTTTTTATCTCCGGGAAATTTGCTCCGGCGAGGGTGTTAGGTTTTTATGGGGGTGTGAGGAGGAGAAGAAGAAGTGTTGGTTTTGTGTTGGAATTTTTAGAAAAGTTTATAGATTCAATAGTTTAAGTTCAACTTGACTCGTTGCTAAGACGTCGGCCGCCATGATGGTCTTTTTCATTTTTTAATTTAAATGCTTATCTTACCTTTTCTTCAAGGTCGTATTAGTTGGTGTCAATTACTTTTGGAAGGGATAGAATAGTTATTGTTTCCCCCGA
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04335 Ps_contig_mira-and-tgicl-ass_franssen_32896
SEQUENCE=GGGGNGGGAGCGTAGACTTTAGTCTTCTCTCCCGCGAGCACATTACAAAGAAACGGCGTCGTTTTGTCCTACATTCCAGCGCACGATTCGAACGGAACAAACTTTCCGACTCCACCAACACAATTTTCCTCCGAATTTCTCGATAAAATCTAGCACAGTCTTCGCAAAGTCTTCTGTATAGATTGCTCACAATCATGGACCGGAGGAGAACCGATAGTCCAGTATACACGCGGCAGTGGAGCGAGAATTCCAGCAGCACCGGCTCATCATCGCCGGTGATGTCGCCGGCTCATCATCAGTCGCGGCTTGGTCCTACTTCTACCGGCATCTCCACCATCAAGCGCACCCAAAACGTCGCTGCTAAAGCCGCCGCGCAGCGCCTTGCTCGCGCCATGGCGTCTCGAACTGTTGACGACGACGAGGATGAGGACGACGATCTCGAATTCGGTTTCAGCGTTCCGCCTGCGTCTGCTCATTCTTCCTTCTCTAGCAACAGGAGTTCTACTGCTAATGCCAACGTCATCCCTCCAATTTCGATCGCGAGGCCGAACAGATCTCCTTCTCCTGCGTTAGGTCGGAACTTTGTAGAGCATACTCAATCAGTGCGTTCATCATCTGCTGGAAGACCAGCAATTTCAGTTCGTTCAGCACAGGTGGTGCCACCTCCCAAATCCACTATCCGAACACCTATGCCTGTACCTCCAATTGATCCTCCTACCAATAGGAATAGAGAAAAAAGGTTTCAACAAGACATCACTATCAGGCAACTTAACTCCAAAGACACAGGAGATCAGCGCGAAGCTTCTGCACTCCGTGATGAACTTGATATGCTACAAGAAGAGAATGATGCTTTATTCGAAAAGCTAAGACACGCAGAGGAAAAACGCCAAGAAGTGGAGATCAGATCCCGGGAACTCGAGAAACAGGTTGCTTCTCTTGGAGAAGGTGTATCCCTGGAAGCCAAACTGCTTAGCAGGAAAGAAGCTGCTTTGCGTCAAAGAGAGGCTGCTCTCTTGGCTGCAAAACAAACACGGAGTGAAAGGGATGAGGATCTCACAGCCCTTCGTGTCGAACTTCAGAACCTAAAAGATGACGCAGCAGCAGCAGAGGAACAACGGCAAGAAGCTGAAGCTGAAGCAAAGGCTCTTCGAACAATGACAGAGAGAATGATTTTGACCCAAGAAGAAATGGAAGAAGTGGTCCTAAAGAGATGTTGGCTTGCCCGCTACTGGGGTCTTTCTGTAAAACATGGCATATGTGCAGATATAGCACAGTCCAAGCATGAACATTGGTCTGCATTAGCTCCTCTTCCTTTTGAACTTGTCACCTCGGCTGGACAAAAGGCTAAGGAGGAATCTTGGAACAAAAGTGCCGATGGTTCAGATAGAAGCAAAATTGTTCGTGATGTGAATGATCTTGCCGGGGAAGGAAACATAGAGAGTATGCTTTCAGTTGAGATGGGTTTGAGGGAACTTGCTTCTTTGAAGGTTGAGGATGCCGTTATACTTGCATTAGCCCAGCACAGACGCCCAAATTTGATTCGGCAGTCTGTTTTAGATTCTAAATCACCTGGTGATGCCAAATATTTGGAAGCATTTGAGTTAAGTGAAGAGGAAGCCGAAGACGTTCTTTTCAAAGAGGCATGGTTAATTTATTTCTGGAGAAGAGCTTTATTTCATGGCGTGGAAGAAGATATTGCAGAAGACAGGCTTCAGTTTTGGATTGCTCGCAATGGACAGTCACCTACTTCACATGATGCTGTTGATGTCGAGCGAGATTTGTTGGAGTTGAGGAAGCTGGGTATAGAACAGCAACTTTGGGAAGCTTCTCGGAAGGGAATTGATCAGCCTTCAGGGTTGTCGACACTTGACAATCATAAACCTGCCTCTGACTCAGATACCTCATCGTGATGGCCGAGCCCTTTATTTGTTGATTGATTCTTTGCCCTTGTATATTTTTGTTTAGTTTTCTATACAAGTTCTTTTACCTTTTCTGTACACCATGTTTTCAACATTATATTTGGGCCTCATAGAAAGGAAGTAAGAATCTTTACGTTGGAAGGAAGACGGTATTTGTGGTAATAAACTACATTGAACTGAGATTCTTTTCCTCGTTCTTTTAATTGAATTTTGTAACTTGTGAAGCTTGTTTGTTAATATTTACATTAGTTTGTTTAGTAGTTTT
TARGET=1095,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0782
PRIMER_LEFT_PENALTY=0.073866
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=GAAAGGGATGAGGATCTCACAG
PRIMER_RIGHT_SEQUENCE=GCCAACATCTCTTTAGGACCAC
PRIMER_LEFT=1037,22
PRIMER_RIGHT=1217,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.0824
PRIMER_LEFT_1_PENALTY=0.078029
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=AAACAGGTTGCTTCTCTTGGAG
PRIMER_RIGHT_1_SEQUENCE=GCCAACATCTCTTTAGGACCAC
PRIMER_LEFT_1=920,22
PRIMER_RIGHT_1=1217,22
PRIMER_LEFT_1_TM=59.922
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.0824
PRIMER_LEFT_2_PENALTY=0.078029
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=AGAAACAGGTTGCTTCTCTTGG
PRIMER_RIGHT_2_SEQUENCE=GCCAACATCTCTTTAGGACCAC
PRIMER_LEFT_2=918,22
PRIMER_RIGHT_2=1217,22
PRIMER_LEFT_2_TM=59.922
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.0907
PRIMER_LEFT_3_PENALTY=0.086339
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=TCGTGTCGAACTTCAGAACCTA
PRIMER_RIGHT_3_SEQUENCE=GCCAACATCTCTTTAGGACCAC
PRIMER_LEFT_3=1063,22
PRIMER_RIGHT_3=1217,22
PRIMER_LEFT_3_TM=59.914
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=0.1149
PRIMER_LEFT_4_PENALTY=0.086339
PRIMER_RIGHT_4_PENALTY=0.028588
PRIMER_LEFT_4_SEQUENCE=TCGTGTCGAACTTCAGAACCTA
PRIMER_RIGHT_4_SEQUENCE=ATTCCTCCTTAGCCTTTTGTCC
PRIMER_LEFT_4=1063,22
PRIMER_RIGHT_4=1355,22
PRIMER_LEFT_4_TM=59.914
PRIMER_RIGHT_4_TM=59.971
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_04336 Ps_contig_mira-and-tgicl-ass_franssen_32899
SEQUENCE=CAGAGTACGCGGNNATAGTTTAATTTTGTTGAGCATTAATGTTTTGTAATTTGAAAAGGAAAAGCCTTAATAATTAAGTCAAGAGTTTGATAGAATACTTTGCTTCTTCACACTGATTTTGATTAGAACAAGCTTTTTTCATTTCGTTTCAATTTCATTTCATTTGAGAGAGAGATGTGGCCTGCGATTATCTGTGGTTTGATTATTTACAAATTGTTCAAATGCTTCTTCTACGACGATGACGTTTCTGACATTGAAGCTTCCGATTCCACTGTTCTCTTCAACGTCGCTGATAGGCTTCAAAAGCTTTTTGGAGGTAAGGTTTTTGTTGGTCTCAGAATTCCCGATGCCGATTCTGCTTCACCACAATCCATTGATATCGTTCTTGTTACTAAACGAGAGCTACTAGTGATATCCGTGAAGAATTTCTCAGGGATTTTGACAGTTCATGGTGATGGTACTTGGCACTCTGAAAAGCCAGGCAAACATAATAAAGTTGAGCGTCACCCTGATCCGGTGGAAGAAGTAAGAAAGCAAGCTTCTATTCTTGAATCCTATCTTGAACAAAGAGGGGTTGTTCTACCTAAAGGGTTTCTAACTTGTAAAGTTATACTTCCAAATCCTAAGTTATGTACCATTCCTACAAGTGATTTTCCACCTGAAGTTATTACCCATGAACAATGGAAGCTACAGAAGCCAGGGACAAAGAGTGTGATCACTAGTTGGGTAAAGAGTGCCTTTCTTAGCGGGAAGAACGATATGCAAGAATCTGGTAATAAGAATCTTGAATTTGTTCTGAGCACAGCTCCAATATGGGATAGGTTGGAACTGAAAGGCAACAAGTATGCATTGGGAGAGTTCCTTGAATTTAAGGGCAAAGCAGAAGATGTTGAGGCATTAAGACATATCAGAAGATCAAAAGTTGGTAGCATGATAATCCAGAAGACAAGCATGTTTGGACTAGCTCCTTCTACGCTTCAAGTTTTATACACTTTGCGCGACTATAGAACTGAGGGAGCATCAGCACCAGAGTTGAATGAAGTGACTGTAAGATCAAATACCGAGATCATCTTTCAGGCAGAAAATGGTACCAAGATCAGAAAGTTTAAGCTCTCTTCAGTCTGCTCTATGCATCTTAGCGCATAAAATTATCTAACTGAGATATCAGAAGAGTGTCAATACAGTAATTTATCTTTATCTTTGACATATAAAATACTATTTTGTGATTGAGTTATATAGATATAGATAGTTGACGGTTTTATTTGAGGGTAGTTACCATTACTAGTAGTTTATTTTATTTTTGGGGATCGTTCTCTCAATAATCAAGAACATTCACTTGCCGACATGAAAATTAGAAAGTGATACCTTCTTTG
TARGET=166,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04337 Ps_contig_mira-and-tgicl-ass_franssen_32912
SEQUENCE=AATCAGGGAAAACGAATCCCCTCCAAGAAACTACATCTATGTTTATCACTGTCGCCANCTTCTTCGTCCATTTTATTGATCATTGGCTGAAACCAAAGCCCTCTTTCCTTTCCGTCTTCTATCTTCGCTTTCTTGAACCTCATCTAGATCATCATCACTCTTTGAACGATTCCTCTTTCTTCTATCTTGCTTTTGTTGTCTTTTTTCTAATTGCTCCTTTTCTTTCTGCCTCTTGTAGTTAAGTTTCTCGGCACATCTGTCACATGCTACCAATTTAACTAGAGCTTGTTTGTTTTCTCCAGCTTCAAAGTAACAAAAGTTCACCTCATAGCTTGCTAATCCATCTTTCTCATTGCAATGCTTGTTACCACATATAAACTGGCCTTTGCCGGACATGACTTCCTTCTCTGTTCTCCATCTGAGACCAATCTTACCACTCTTGTACTGTGACATGTCAGCAAGACAGTATTCTTTAAAAAGCTTGGCATAATAGCGCTTCACAAGCCTTTGCTCCCATGATGGATCCATATCATCTTCCTCTGACCGTATGAATCTGTATCCCTCTCTTAGGGTATCTTTATCTGTTTTAATAGGCAGCTTTAAGGTTGAAGGTTTTTCTTCTTTCCCATAGAAACTAACGTAATCTTTAATGATTTTTTATGGCGATCGTATGCATTGAGGCCTCGAATATGCGCCTGATATTGCTGTTTTCTCTCTTCTTTGTCGAATATAGCTGATTTAAGAGATCCAAACGATTGTCATGATTTCGCCGCCGCCGCCGCCGCCGTCAAGACGGAACCGCAATGAGATTTCGGAAGCAGGGTTGTTTGGGGAAGAACGCTTAAATGGCGTTTCGTTTTTTACACTTCCCCGCG
TARGET=768,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5188
PRIMER_LEFT_PENALTY=0.843614
PRIMER_RIGHT_PENALTY=0.675162
PRIMER_LEFT_SEQUENCE=ATATGCGCCTGATATTGCTGTT
PRIMER_RIGHT_SEQUENCE=ATTTAAGCGTTCTTCCCCAAAC
PRIMER_LEFT=687,22
PRIMER_RIGHT=846,22
PRIMER_LEFT_TM=60.844
PRIMER_RIGHT_TM=60.675
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=1.5188
PRIMER_LEFT_1_PENALTY=0.843614
PRIMER_RIGHT_1_PENALTY=0.675162
PRIMER_LEFT_1_SEQUENCE=AATATGCGCCTGATATTGCTGT
PRIMER_RIGHT_1_SEQUENCE=ATTTAAGCGTTCTTCCCCAAAC
PRIMER_LEFT_1=686,22
PRIMER_RIGHT_1=846,22
PRIMER_LEFT_1_TM=60.844
PRIMER_RIGHT_1_TM=60.675
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=161
PRIMER_PAIR_PENALTY_2=1.7707
PRIMER_LEFT_2_PENALTY=1.095522
PRIMER_RIGHT_2_PENALTY=0.675162
PRIMER_LEFT_2_SEQUENCE=AATATGCGCCTGATATTGCTG
PRIMER_RIGHT_2_SEQUENCE=ATTTAAGCGTTCTTCCCCAAAC
PRIMER_LEFT_2=686,21
PRIMER_RIGHT_2=846,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.675
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=1.7771
PRIMER_LEFT_3_PENALTY=1.101902
PRIMER_RIGHT_3_PENALTY=0.675162
PRIMER_LEFT_3_SEQUENCE=CGCCTGATATTGCTGTTTTCTC
PRIMER_RIGHT_3_SEQUENCE=ATTTAAGCGTTCTTCCCCAAAC
PRIMER_LEFT_3=692,22
PRIMER_RIGHT_3=846,22
PRIMER_LEFT_3_TM=61.102
PRIMER_RIGHT_3_TM=60.675
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=1.9717
PRIMER_LEFT_4_PENALTY=1.296517
PRIMER_RIGHT_4_PENALTY=0.675162
PRIMER_LEFT_4_SEQUENCE=GCTGTTTTCTCTCTTCTTTGTCG
PRIMER_RIGHT_4_SEQUENCE=ATTTAAGCGTTCTTCCCCAAAC
PRIMER_LEFT_4=703,23
PRIMER_RIGHT_4=846,22
PRIMER_LEFT_4_TM=59.703
PRIMER_RIGHT_4_TM=60.675
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_04338 Ps_contig_mira-and-tgicl-ass_franssen_32918
SEQUENCE=TTACCCACGCTGGGARGCAGTCATTTCTTGCTATACATTGAATCGAAACAACATTACATCTCCTTCTTGTACAATGTAGTCTTTGCCTTCAGATCTCAAAAGTCCTTTTTCTCGTGCTGCAGCAAGTGAACCAGCAGCAACAAAGTCATTATAAGCCACTGTCTCTGCTCGAATGAAACCCTTCTCAAAGTCAGAATGAATAACTCCAGCAGCTTGAGGCGCAGTCATTCCTGCAAGTATTGTCCATGCTTTTGTCTCCTTCTCACCAGAAGTAAAATAAGTCCGCAGCCCCAAGAGATCATAGGTTGCTCTAATTAGGTTACCAAGACCACTTTCACTAACGCCAAGGGATTTCAAATATTCTTGCCTTTCCTCAATTGCTAGTTCAGAAAGCTCTGCCTCAACCTGTGCTGAAATTGTTACAATTCCTGACTTCAACTCAGATGCAACATTCTTGACACCTACAACGTAATCATTGTTTGTAGCATCAGCTAGATCAGTCTCCGCAACATTTGCCACATATATAACGGGCTTCATGGTGAGCAAGCAGAGATTCTTTATAGCATCCTTTTCAAAGTCTGTTAAGGTCACAGATCGTGCAGGTTTTCCATCCAAGAGTGCTACGCTAATTTTTTCCAATGCAGCCTTTTCTGCCTCTTCCTTGACTTTAGATTGTGAATCCTTTGCCTTGTCTTTTTTTTAGTTTATCTAGTCTTTTCTCAATCTGGTCTAAGTCTGTGAAAACAAGCTCCAAGTTGATAACATCTATATCAGATTTGGGATCAACTTTCCCATTCACATGAATAATGTCATTGTCTTCAAAACATCTAACAACCTGAAGTATGGAGTCCACCTCACGAATATGCGATAAAAACTTATTACCAAGCCCCTCGCCTTGACTTGCACCCTTGACAAGCCCAGCAATATCTACAAACTCTATAGACGCTGGAACAACTCGTTGAGATTTACTGAGGTTGGAGAGCACATGGAGACGAGAATCTGGAACGGCAACAATCCCTACGTTTGGCTCAATTGTACAAAAAGGAAAATTAGCAGCTTGAGCCTTTCCATTTTCAACAACGGCATTGAAGAGGGTAGACTTGCCAACATTGGGGAGACCAACAATACCAGCCTTGAGGCTCATGCTTATCTTGGATGAAGAAGAAGAATAAGAAAGCTTGGGTTGAACTCCATAAAGTTGAAAAGAGTGTTTGGTTAAAAGAGTATGATGATTACCGAAAAACCTTGACTTCAATGGATGAACTAAACTGGGAACTAGTTTAGCCATAATTGTTAGGAGAATTTGAGAGCAGAGAGTGTGAGTTTGGGTTGTTATCTTTGCTGTTCTACGCGCATTTTCAATGGATGCAAGTGCTTTATCTCTATGCGGTTTATCTATGTCYGC
TARGET=1158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04339 Ps_contig_mira-and-tgicl-ass_franssen_32937
SEQUENCE=ATAAAAGGTGAAAAATCAAYGCRNAGTRNTCGGGGACAACATAAATTGAAGGAAAAGAAAAAGGGGAACGAACGAACGAAAGAACCAGTGAGAGCGGTGAAGAGACCGAAACCCTAAGCCAGAGATGGCGGAGCATTTGGCATCGATCTTCGGAACCGAGAAAGACAGAGTTAACTGCCCTTTCTACTTCAAGATCGGAGCTTGCAGACATGGCGACCGTTGTTCTAGGCTTCACACTAAGCCCACCATTAGTCCCACTCTCGTTCTCTCCAACATGTATCAGAGACCTGACATGAATCTCAACTTCATCAACCCTACCCCTAATCAGCCTCAGCAACCTCAACCACCTCAGCAGCCGCAGCCGGAATCGCTCGACCCTGATAAGCTTCAGGAACACTTCGATGATTTTTATGAAGATCTGTTTGAGGAGCTTAGCAAGTATGGGCAAATTCAGAGCTTGAATATTTGTGACAATTTGGCTGATCACATGGTTGGTAATGTATATGTTCAGTATAAAGAGGAAGATCATGCTGCCAATGCCCTAATGAATCTCACTGGAAGNATTTTAAACTAACAGGTACGACCGATCATTTGTTGACTTCTC
TARGET=66,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04340 Ps_contig_mira-and-tgicl-ass_franssen_32945
SEQUENCE=GGGGTGCCGTCTTCAGCTTCCATGGCTCTACACCATTTATTACAATTCTAACTGAATCTACCTGTTCCCTTCAATCCTTATAACACACTACACACATGATTTCTCTTGAACCGCAAAAGTTGTTGCTCAACATTTAACACAACCTATTCAATTCAAACATAAACCTATCCACTTTCTATCTCTCTTCTTCACCGATAGCTAATGGATTACACACATTTCTTCCTACTAGCAACAACCACTTTAGGTTTCATCTCCATCTCCAAATCTCTCTTCAATTTTCTCGTCTGGATATACGCCACCTTAATCCGACCAACAAAGCATCTCAAAGACTACGGTTCATGGGCTATTATCACCGGCTCAACCGACGGAATCGGCAAATCCATGGCTCTCGAGCTCGCAGCCAACGGACTCAACCTTCTCTTACTCGGTCGAAACCCTCTTAAGCTCGAAGCAACGTCAAAAGAGATAGTAGACAAACATTTCACCAACGTTCAGGTAAAATATCTTGTTGTAGACCTGCAGAACAATAGCGGTGACGAAATAATGAAGAAAATTGAAGAAGCTATTGCTGATTTAGATGTTGGAATTCTAGTTAATGCCGCCGGCGTGGCCTATCCTTATGCTAGATATTTTCATGAAGTTGATTTAGATTTGATGGATACTATTATTAAAGTCAATATAGAAGGAACAACTTGGGTTACAAAAGCTGTGATTCCTAGTATGATTAAGAAGAAAAAAGGAGCTATAATTAACATCGGTTCTGGTTCCACTGTTGTTCTTCCTTCTTATCCTCTTGTCACACTTTATGCAGCTTCAAAATCGTATCTTGCCATGTTCTCTGCATGCACCAATTTGGAATACAAACACCAAGGTATTGACATCCAGTGTCAGGTTCCGTTGTTTGTGTCGACGAAAATGACAAGGATGAAGGCTTCTCTGTTTGTTCCAACACCAGATGTATATAGCAAAACATGTATGAAATGGATTGGTTATGAGAAACTGGCTGTACCTTACTTTTTTCACTCTTTGCAGTCGTTTGTCATAAGGGCGATTCCTGATGTGTTGTTGGATTCGTATATGCTCAATTATTTTCTTTACTGGCGTAAAAGAGGACTAGTTAAGGACTCGCAAATTAAAGGAACTAGTAATTCTGAAGCAAATTAGCATTGATGATGATGATGATGCCAAATTTAGTTTTATGTTTCTGCTAAATCAACATCACTGTAAAAGATAAGAGTCATTAGGATGGTCCCAGTTCGTAGTATCCTTTTTTGTATTTTCTTGATCATTTGCTTAAAACTATTATTGCTGTTTTCTTAATGTAGCTCTAATTTGTTTTGTTATTTTGAGGTATCCTGTGTGTATTAATAGTCTTAACCATGTAGTTCTGTTGCTATTTATGAGTCCTGAATCCAACTAATTTGTTATGRAACC
TARGET=1168,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04341 Ps_contig_mira-and-tgicl-ass_franssen_32952
SEQUENCE=CGGTCGGGGNCGCTNATCTGAGTCCGAAGCAAGCTACCGGAGAAGGACAGAGAGAGAGAGAAAGATCGCAATTCATCACATCACACCAAAACGAAGAAATCAAAATAGAAAAATCCTTCGATCGTTTCCATAGTCGAAACGAATCGAAACGAATCGAATCGAATCGAATCGAGCAAGCAAAGATGTTACCAACCGTAACCAAAGGACGTTCTTCTTCCTCTTTATCTTCATCCTCTTCTAGACCTAATTCCACTCTTCTTCCTTACCTCCGAAGAATTGTCAAGTGGCAACAAATGGACGTTGAATATACTTTTTGGCAAATGCTTCATCTATGTACGTCACCAAAAGTTGTCTATCAGCATACAAAATATCACAAACAAACCAAGAACCAATGGGCACGCGATGACCCTGCATTTGTTGTGATCTGCAGCTTTCTTTTGGCTGTAGCAACTTTGGCTTATTGTGCAGCGTATGACCATAGTTCTGGTCATGCACTTTTTGTTGTCTTCTCAGTGTTGCTTTTCCATTTTCTATTGACTGGCATATTTCTGGCAACCTTTTGTTGGTTTCTAACTAATTCTTATCTTCGAGAAGAGGCTCCAAACAGTTATGTGGTTGAACAGCGTGTTGAATGGATGTATGCATTTGATGTTCACTGCAACTCTTTCTTCCCAATGTTTGTTTTGCTATATGTGATCCATTATTTTCTATCTCCTCTATTGGTGGCTCATGGCTTCATTCCAGAACTGCTATCAAATCTATTATTCATGGTGGGAGCTTCATACTACCATTATCTAAATTTCTTAGGTTATGATGTTCTGCCCTTTTTGGAGAGGACCACCTTTTTTCTGTATCCAATTGGTGTAGTGATTGTTCTTTCTCCCATATTGATTTTGAGTGGCTTCAATCCTTCTAGATACTTTATGAGCATATACTTCAGCCGGCAGATATGATCATTCCAGTTAAAACTTTTGACCAGTCTGTGTCATGTTGAGGTTATAAGTAATGGAGACTACAAAATGAGAGAAGCTTTAATGAATGTTTGGTTGTTTGTTCGACGAGTGCAATACCCGGCAATGCATCATTTTAGACCTTCTAGTATAAATCGAAGTTGAACTGTAGTAAGTCAGGGTAGTCAGTGTATCTTTTACAGATATTAAATAGAATTTGTTCAGATGAATACAATCATTGTAATCAATTTTCATTGTCAATTCAGCATTTTTCCTTCCAACAAAG
TARGET=49,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7685
PRIMER_LEFT_PENALTY=0.647803
PRIMER_RIGHT_PENALTY=0.120688
PRIMER_LEFT_SEQUENCE=NATCTGAGTCCGAAGCAAGCTA
PRIMER_RIGHT_SEQUENCE=TCGGAGGTAAGGAAGAAGAGTG
PRIMER_LEFT=14,22
PRIMER_RIGHT=271,22
PRIMER_LEFT_TM=60.648
PRIMER_RIGHT_TM=59.879
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.7901
PRIMER_LEFT_1_PENALTY=0.647803
PRIMER_RIGHT_1_PENALTY=0.142283
PRIMER_LEFT_1_SEQUENCE=NATCTGAGTCCGAAGCAAGCTA
PRIMER_RIGHT_1_SEQUENCE=GTCCTTTGGTTACGGTTGGTAA
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=206,22
PRIMER_LEFT_1_TM=60.648
PRIMER_RIGHT_1_TM=60.142
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.8018
PRIMER_LEFT_2_PENALTY=0.647803
PRIMER_RIGHT_2_PENALTY=0.154023
PRIMER_LEFT_2_SEQUENCE=NATCTGAGTCCGAAGCAAGCTA
PRIMER_RIGHT_2_SEQUENCE=TGTTGCCACTTGACAATTCTTC
PRIMER_LEFT_2=14,22
PRIMER_RIGHT_2=291,22
PRIMER_LEFT_2_TM=60.648
PRIMER_RIGHT_2_TM=60.154
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.9576
PRIMER_LEFT_3_PENALTY=0.647803
PRIMER_RIGHT_3_PENALTY=0.309780
PRIMER_LEFT_3_SEQUENCE=NATCTGAGTCCGAAGCAAGCTA
PRIMER_RIGHT_3_SEQUENCE=AGAAGAACGTCCTTTGGTTACG
PRIMER_LEFT_3=14,22
PRIMER_RIGHT_3=214,22
PRIMER_LEFT_3_TM=60.648
PRIMER_RIGHT_3_TM=59.690
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=1.0547
PRIMER_LEFT_4_PENALTY=0.647803
PRIMER_RIGHT_4_PENALTY=0.406861
PRIMER_LEFT_4_SEQUENCE=NATCTGAGTCCGAAGCAAGCTA
PRIMER_RIGHT_4_SEQUENCE=GACAATTCTTCGGAGGTAAGGA
PRIMER_LEFT_4=14,22
PRIMER_RIGHT_4=280,22
PRIMER_LEFT_4_TM=60.648
PRIMER_RIGHT_4_TM=59.593
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_04342 Ps_contig_mira-and-tgicl-ass_franssen_32952
SEQUENCE=CGGTCGGGGNCGCTNATCTGAGTCCGAAGCAAGCTACCGGAGAAGGACAGAGAGAGAGAGAAAGATCGCAATTCATCACATCACACCAAAACGAAGAAATCAAAATAGAAAAATCCTTCGATCGTTTCCATAGTCGAAACGAATCGAAACGAATCGAATCGAATCGAATCGAGCAAGCAAAGATGTTACCAACCGTAACCAAAGGACGTTCTTCTTCCTCTTTATCTTCATCCTCTTCTAGACCTAATTCCACTCTTCTTCCTTACCTCCGAAGAATTGTCAAGTGGCAACAAATGGACGTTGAATATACTTTTTGGCAAATGCTTCATCTATGTACGTCACCAAAAGTTGTCTATCAGCATACAAAATATCACAAACAAACCAAGAACCAATGGGCACGCGATGACCCTGCATTTGTTGTGATCTGCAGCTTTCTTTTGGCTGTAGCAACTTTGGCTTATTGTGCAGCGTATGACCATAGTTCTGGTCATGCACTTTTTGTTGTCTTCTCAGTGTTGCTTTTCCATTTTCTATTGACTGGCATATTTCTGGCAACCTTTTGTTGGTTTCTAACTAATTCTTATCTTCGAGAAGAGGCTCCAAACAGTTATGTGGTTGAACAGCGTGTTGAATGGATGTATGCATTTGATGTTCACTGCAACTCTTTCTTCCCAATGTTTGTTTTGCTATATGTGATCCATTATTTTCTATCTCCTCTATTGGTGGCTCATGGCTTCATTCCAGAACTGCTATCAAATCTATTATTCATGGTGGGAGCTTCATACTACCATTATCTAAATTTCTTAGGTTATGATGTTCTGCCCTTTTTGGAGAGGACCACCTTTTTTCTGTATCCAATTGGTGTAGTGATTGTTCTTTCTCCCATATTGATTTTGAGTGGCTTCAATCCTTCTAGATACTTTATGAGCATATACTTCAGCCGGCAGATATGATCATTCCAGTTAAAACTTTTGACCAGTCTGTGTCATGTTGAGGTTATAAGTAATGGAGACTACAAAATGAGAGAAGCTTTAATGAATGTTTGGTTGTTTGTTCGACGAGTGCAATACCCGGCAATGCATCATTTTAGACCTTCTAGTATAAATCGAAGTTGAACTGTAGTAAGTCAGGGTAGTCAGTGTATCTTTTACAGATATTAAATAGAATTTGTTCAGATGAATACAATCATTGTAATCAATTTTCATTGTCAATTCAGCATTTTTCCTTCCAACAAAG
TARGET=150,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0758
PRIMER_LEFT_PENALTY=0.009409
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=TACCGGAGAAGGACAGAGAGAG
PRIMER_RIGHT_SEQUENCE=ACGGTTGGTAACATCTTTGCTT
PRIMER_LEFT=34,22
PRIMER_RIGHT=195,22
PRIMER_LEFT_TM=60.009
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=162
PRIMER_PAIR_PENALTY_1=0.0758
PRIMER_LEFT_1_PENALTY=0.009409
PRIMER_RIGHT_1_PENALTY=0.066394
PRIMER_LEFT_1_SEQUENCE=CTACCGGAGAAGGACAGAGAGA
PRIMER_RIGHT_1_SEQUENCE=ACGGTTGGTAACATCTTTGCTT
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=195,22
PRIMER_LEFT_1_TM=60.009
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.0974
PRIMER_LEFT_2_PENALTY=0.030986
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=AGATCGCAATTCATCACATCAC
PRIMER_RIGHT_2_SEQUENCE=ACGGTTGGTAACATCTTTGCTT
PRIMER_LEFT_2=62,22
PRIMER_RIGHT_2=195,22
PRIMER_LEFT_2_TM=59.969
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=0.1301
PRIMER_LEFT_3_PENALTY=0.009409
PRIMER_RIGHT_3_PENALTY=0.120688
PRIMER_LEFT_3_SEQUENCE=TACCGGAGAAGGACAGAGAGAG
PRIMER_RIGHT_3_SEQUENCE=TCGGAGGTAAGGAAGAAGAGTG
PRIMER_LEFT_3=34,22
PRIMER_RIGHT_3=271,22
PRIMER_LEFT_3_TM=60.009
PRIMER_RIGHT_3_TM=59.879
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.1301
PRIMER_LEFT_4_PENALTY=0.009409
PRIMER_RIGHT_4_PENALTY=0.120688
PRIMER_LEFT_4_SEQUENCE=CTACCGGAGAAGGACAGAGAGA
PRIMER_RIGHT_4_SEQUENCE=TCGGAGGTAAGGAAGAAGAGTG
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=271,22
PRIMER_LEFT_4_TM=60.009
PRIMER_RIGHT_4_TM=59.879
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_04343 Ps_contig_mira-and-tgicl-ass_franssen_32957
SEQUENCE=TCTCTCTCTCTCTCATCTTTATCNCTCNCTAAATCTATGTGTNTAATAAAGAATTATGAACGTGTAGGAGCGAAAGATTACTACGAAAAACAAATCGCTACTCTGAAATCATTTGAGGAAGTGGATGCGGTGGTGGAATCTGACGGCATTATTGAGGAAGATAAAGAAGAACTGGCTCAACAAGAAAGAGCAATGAAGATTTCAAATTATGCAAATATAGTTTTGTTGATATTGAAGGTTTATGCGACGGTAAGGAGTGGGTCAATAGCTATTGCAGCATCAACTTTGGATTCTCTGCTTGATCTAATGTCTGGTGGAATACTTTGGTACACTCACTTAGCAATGAAGAACATAAATATCTACCAGTATCCCATTGGAAAGCTGAGGGTTCAGCCGGTAGGCATAATTGTTTTCGCCGCTATCATGGCCACACTTGGATTTCAGGTGCTGGTTACGGCCTTAGAACAACTAATAAAAAACAGTCCTAGTGAAAAGATGACGGGGGAACAGTTAATATGGTTGTATTCTATCATGATATTTGCAACTGTGGTGAAGCTTATACTTTGGCTTTACTGCAGAAGCTCGGGTAACCAGATTGTTCGTACCTACGCGGATGATCACCACTTTGATGTGGTAACAAATGTAGTTGGGTTAGTTGCGGCTATTCTTGGTGATAAGTTTTATTGGTGGATTGATCCGGTTGGAGCTATTTTACTTGCAATTTACACTATTACCAATTGGTCTCGCACTGTCATGGAAAATGCAGTTTCACTGGTGGGACAATCTGCGTCTCCTGAATTTTTGCAGAAGTTAACATATCTCGTTATAAGGCACCCTGGAATTAAACGCGTTGACACTGTCCGCGCATACACATTTGGCGTTTTATATTTTGTGGAGGTAGACATTGAACTTCCAGAAGATCTACCCTTAAAAGAATCACATGCTATTGGGGAGACTCTACAGATAAAGCTTGAGAAACTTTCAGAAGTTGAGCGAGCATTTGTTCATCTGGACTTTGAGTGTGATCACAAACCAGAGCACTCAATTCTCAGTAAACTACCCAACAATTAGCCTTAAGCACACCTTTTCCTGTCCACGCACAGGAAGAAATGCGAACTTTTGCAATTGAGTTTTCATCGACAACTTCTTTGTGAAGAGTGAAACTTTAAAATAGCAGGATCCGGTTGTTTGGTTATATGAAAGCTGAAACTGCAACCAACTTTTGCCCAACTTCAACTTACTTTTGGTGCAAATACTTTCTGTAATTGTCTTATTGGCCAGTTGCAAAGTTTTGAAAGTTTCATTAGGTTCCATACTAGTTCAAAAGTTCATATCGAAATCACACATGTTATGTTAAAGGTTATTGTCGTGTTGTATCCTTAAGATTATTCAATAGAATATAAAAAGTAAATTTTATT
TARGET=1,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04344 Ps_contig_mira-and-tgicl-ass_franssen_32959
SEQUENCE=TTATCAAATATTTANTTAAATGTAAGATATTCCATCGCGTATCAAGATTGGGGACGAAAATGGTACTGCAAATTTACTACTAGGTACTACTATTTTATTAACTTAATTAAACTTAATTAAGTTACTCCAAATCCAAGATTAAATCATAATCAAAATTCATTATTCAGCATGGAAGCACCTTGTAGATTACCACGAAACAAGGAAGGATAGCCCTTGGATTGAAAACTGTTAAATCCTCGAGATTCGAGTAGATTCCCGCGTCCCCCGCAACAGAATCAAACGACATCGTTTCCTCCTCCGTTATCGGTGGCGCACCTTCCTCCGCCACGCGCTTCACTTTTCCGGCAATCACACGGCACACCAGCATCGCTCTTCTAGAATCTTCTCCAATCCTTTCAATGGAATTGTCGTGGGCCTTACCACTGCTAGCGTTGGTTTTCACACCTCCTTTGTTCTGAAAACCGTGCCGGATGATCGTGCACACTCCGCAACCAGGTACGGTGGCGCACAAGGCGGAGGATCCACGTGCGCCTAGTGCGCAGGATAAGGTTGTGCTGTGGAAACGAAGGAGTTCGTTGCCGTCTGCGGCACACCGGGGGTTTTTCTTTGTAGTTGCGGTTGCACGGGATTTTACTGCGTCTCTGCATTCCTCGAACCGTTGGATTGTTCGTTGAGTGTTGTGAACTTTTAATATCCGTTCGATCTTACACATTGGACTCTCCCTCTTCAACCAGCTTGACTTGAATATTATTTCCACTATGTTCCGACCCGAATCTTCCGGACCCAGCTCTGAAACAGCATGACGAACAGCTTGATGAAGCTGCAAGCTTTCAATCTTAGGGAAAACCTCTCCACATTGATCACAAGCACACACTGTGCTCCTAAGGAATTGCTAACCTGCTAGGGTCAACGATCATGTGACATTCATAACATCCAGAGAGTTTTCTAAACTGCATTCCTCTGGAAGATGATGACGTGTAGTTATTTCCACCGTTGGATTTCCCAGAACATGATGCAGAAGAGCGAGTTCTTGATGATGATGACGATGAAGCAGTGGTAGTGACAGGTTTTCTTTTGAGTAAACTTGTTTCTTGACCTAAGATGCTACTTTCTGGGGATGAATTATCAGACCTATGAACAACTCGAGTGTTTCCATGAACGGCATCTCTGAAGCTACAAATTGAGCTACAAGATGAACCTAGTTTTGAGTAACTGTTGTTTGGTTTTGAAGGATCGTGAACGGTGGAGCCTTCGATCTGTTTGCAAGTTAGGATGTTTTTGATTTGGTCCCATGAAGATGGTGGCTTTTGTTTTTGTTTTTGTTTCTGTTTTTGTTTTTGCTTTTGATTCAGTTGTTGTTGTTGTTGGGTTTTGTGTTTTTGTTTTGGGAGTTGTTTTTTNGGAGTGAGATTGTTCAGAGGGATTAGTGAGGAGAGCCATTAGGAAGAGTAGAAAAGAAAATGTACTTCCCTTCTTTCATAAGG
TARGET=1032,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1143
PRIMER_LEFT_PENALTY=0.080231
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=CACATTGATCACAAGCACACAC
PRIMER_RIGHT_SEQUENCE=AATTCATCCCCAGAAAGTAGCA
PRIMER_LEFT=852,22
PRIMER_RIGHT=1123,22
PRIMER_LEFT_TM=60.080
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.1144
PRIMER_LEFT_1_PENALTY=0.080323
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=TGACGTGTAGTTATTTCCACCG
PRIMER_RIGHT_1_SEQUENCE=AATTCATCCCCAGAAAGTAGCA
PRIMER_LEFT_1=971,22
PRIMER_RIGHT_1=1123,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=153
PRIMER_PAIR_PENALTY_2=0.1366
PRIMER_LEFT_2_PENALTY=0.102490
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=TCTTAGGGAAAACCTCTCCACA
PRIMER_RIGHT_2_SEQUENCE=AATTCATCCCCAGAAAGTAGCA
PRIMER_LEFT_2=834,22
PRIMER_RIGHT_2=1123,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.1758
PRIMER_LEFT_3_PENALTY=0.080231
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=CACATTGATCACAAGCACACAC
PRIMER_RIGHT_3_SEQUENCE=TCATCCCCAGAAAGTAGCATCT
PRIMER_LEFT_3=852,22
PRIMER_RIGHT_3=1120,22
PRIMER_LEFT_3_TM=60.080
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=0.1759
PRIMER_LEFT_4_PENALTY=0.080323
PRIMER_RIGHT_4_PENALTY=0.095574
PRIMER_LEFT_4_SEQUENCE=TGACGTGTAGTTATTTCCACCG
PRIMER_RIGHT_4_SEQUENCE=TCATCCCCAGAAAGTAGCATCT
PRIMER_LEFT_4=971,22
PRIMER_RIGHT_4=1120,22
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_04345 Ps_contig_mira-and-tgicl-ass_franssen_32959
SEQUENCE=TTATCAAATATTTANTTAAATGTAAGATATTCCATCGCGTATCAAGATTGGGGACGAAAATGGTACTGCAAATTTACTACTAGGTACTACTATTTTATTAACTTAATTAAACTTAATTAAGTTACTCCAAATCCAAGATTAAATCATAATCAAAATTCATTATTCAGCATGGAAGCACCTTGTAGATTACCACGAAACAAGGAAGGATAGCCCTTGGATTGAAAACTGTTAAATCCTCGAGATTCGAGTAGATTCCCGCGTCCCCCGCAACAGAATCAAACGACATCGTTTCCTCCTCCGTTATCGGTGGCGCACCTTCCTCCGCCACGCGCTTCACTTTTCCGGCAATCACACGGCACACCAGCATCGCTCTTCTAGAATCTTCTCCAATCCTTTCAATGGAATTGTCGTGGGCCTTACCACTGCTAGCGTTGGTTTTCACACCTCCTTTGTTCTGAAAACCGTGCCGGATGATCGTGCACACTCCGCAACCAGGTACGGTGGCGCACAAGGCGGAGGATCCACGTGCGCCTAGTGCGCAGGATAAGGTTGTGCTGTGGAAACGAAGGAGTTCGTTGCCGTCTGCGGCACACCGGGGGTTTTTCTTTGTAGTTGCGGTTGCACGGGATTTTACTGCGTCTCTGCATTCCTCGAACCGTTGGATTGTTCGTTGAGTGTTGTGAACTTTTAATATCCGTTCGATCTTACACATTGGACTCTCCCTCTTCAACCAGCTTGACTTGAATATTATTTCCACTATGTTCCGACCCGAATCTTCCGGACCCAGCTCTGAAACAGCATGACGAACAGCTTGATGAAGCTGCAAGCTTTCAATCTTAGGGAAAACCTCTCCACATTGATCACAAGCACACACTGTGCTCCTAAGGAATTGCTAACCTGCTAGGGTCAACGATCATGTGACATTCATAACATCCAGAGAGTTTTCTAAACTGCATTCCTCTGGAAGATGATGACGTGTAGTTATTTCCACCGTTGGATTTCCCAGAACATGATGCAGAAGAGCGAGTTCTTGATGATGATGACGATGAAGCAGTGGTAGTGACAGGTTTTCTTTTGAGTAAACTTGTTTCTTGACCTAAGATGCTACTTTCTGGGGATGAATTATCAGACCTATGAACAACTCGAGTGTTTCCATGAACGGCATCTCTGAAGCTACAAATTGAGCTACAAGATGAACCTAGTTTTGAGTAACTGTTGTTTGGTTTTGAAGGATCGTGAACGGTGGAGCCTTCGATCTGTTTGCAAGTTAGGATGTTTTTGATTTGGTCCCATGAAGATGGTGGCTTTTGTTTTTGTTTTTGTTTCTGTTTTTGTTTTTGCTTTTGATTCAGTTGTTGTTGTTGTTGGGTTTTGTGTTTTTGTTTTGGGAGTTGTTTTTTNGGAGTGAGATTGTTCAGAGGGATTAGTGAGGAGAGCCATTAGGAAGAGTAGAAAAGAAAATGTACTTCCCTTCTTTCATAAGG
TARGET=1352,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3439
PRIMER_LEFT_PENALTY=0.117281
PRIMER_RIGHT_PENALTY=0.226649
PRIMER_LEFT_SEQUENCE=TCGATCTGTTTGCAAGTTAGGA
PRIMER_RIGHT_SEQUENCE=GGCTCTCCTCACTAATCCCTCT
PRIMER_LEFT=1251,22
PRIMER_RIGHT=1437,22
PRIMER_LEFT_TM=59.883
PRIMER_RIGHT_TM=60.227
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.5835
PRIMER_LEFT_1_PENALTY=0.117281
PRIMER_RIGHT_1_PENALTY=0.466222
PRIMER_LEFT_1_SEQUENCE=TCGATCTGTTTGCAAGTTAGGA
PRIMER_RIGHT_1_SEQUENCE=ATGGCTCTCCTCACTAATCCCT
PRIMER_LEFT_1=1251,22
PRIMER_RIGHT_1=1439,22
PRIMER_LEFT_1_TM=59.883
PRIMER_RIGHT_1_TM=60.466
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=0.5879
PRIMER_LEFT_2_PENALTY=0.361206
PRIMER_RIGHT_2_PENALTY=0.226649
PRIMER_LEFT_2_SEQUENCE=CAACTCGAGTGTTTCCATGAAC
PRIMER_RIGHT_2_SEQUENCE=GGCTCTCCTCACTAATCCCTCT
PRIMER_LEFT_2=1138,22
PRIMER_RIGHT_2=1437,22
PRIMER_LEFT_2_TM=59.639
PRIMER_RIGHT_2_TM=60.227
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.6217
PRIMER_LEFT_3_PENALTY=0.598109
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=CCTTCGATCTGTTTGCAAGTTA
PRIMER_RIGHT_3_SEQUENCE=CTCTTCCTAATGGCTCTCCTCA
PRIMER_LEFT_3=1248,22
PRIMER_RIGHT_3=1448,22
PRIMER_LEFT_3_TM=59.402
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=0.6217
PRIMER_LEFT_4_PENALTY=0.598109
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=TTCGATCTGTTTGCAAGTTAGG
PRIMER_RIGHT_4_SEQUENCE=CTCTTCCTAATGGCTCTCCTCA
PRIMER_LEFT_4=1250,22
PRIMER_RIGHT_4=1448,22
PRIMER_LEFT_4_TM=59.402
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_04346 Ps_contig_mira-and-tgicl-ass_franssen_32961
SEQUENCE=AGAGAAAGAAAACAACTTCAAATCAAGTAATGTTCAAGATCAATGACAACTTCAACATAACAATTATTGATTACACATTATAATATGAATAAATAAGAGGAAGACATGTTCTAATTACTATACTAAAGTATTTTACAATTAGATAAAAAAGCAAGAATTTTTCTTGCAAAACTAGAATCTCAGCTAAAAATGTATAAGGCAATATATATATTTTAAAAAATAACCATAAAATAACAGCTGCGGCGGGTACACGACACATCATCACCATCACACGCGAAGAAACTTAACCATTTTTAGATACCAAAATTGCAGGACTGAACAAAGTCTAACTCGTAATCGACATACTTAGACCAAAGTGGTTTGAATTGAGTCATTGCAATATCAAGCCATGGTTTCATGTTACCATTGAAATGCACCACAGCTGCATTGTTAATCTCATCCATGCTGATGCTTGGGTTATAACCAAGTCCCAAAACATGCCATGATTTATCTAGCGGTTTCGTTGTTGAGTAATATGTGATTAGCCCGGGTGGTAATGTCCCTAGTTTCCATAGTGTCCGGTTCTCGTTCAGATTTTGCCAGTAATGATACTCTTCTGTGCATTTTTCCCTTCTCCAAGCATCCAAATCGAAGAAATTCATTCCATAAGCCCATGCACAAGTCTTTGGATTAAATCGAGCTTTGATCAAAGGATGCGAGAAATTCATGTACTGTGCATATCTATGGAATGACCCGAAACATGTTTCTACAGCTCCATTCACCTTGCCATCCATGTCAATCTTCCATAAACCAGTAAGGTCCTTCTGAACTACTATGTCATCGTCCAAAAATAACACTTTATGCAACTTCGGATACATTTCCGGCAAGTAGAATCTCAAATGGTTCAATATCGATAAATACTTTGGATTCCTGAACTTCATATTTGTTGTGTCCTTTGTAGCATTCTCGAGCTTGTTTTCAAAGTAAAACCTCTGTAGGTTAGCAGACTCGAGCTGTCGAAGGACCGGCACGTAAGAAGAATTCAGGAATTTGTAATCCTCAACTGCCTTAACTTCAACGTGTGCGCCGTTGTAATCTTTTAACTTGAACATCACTTGCATAGCTCCAAGATTCATCTTGTCAGTCACAACATGAAACACGTGTTTCCATGGTTCCTTCGCGTTCTTTGTCGCTGAATTAACCACAACAGATGCAGCCACAACGTTGTCCGAGAACAACGCGTAGTGGTAAAGATTAGGATCTTCAACTTCAGGAGGAGTAGGCTTCCCTTCATCTGTATACTTTTCCGGATGAGCAATCCGCTCTTCCATCAGTCTCATTGTTAGACAATGCAAACTCTTCGGAATGGACTTGGCAGCAATCAAACTCGAGAAAGCACCCTGCTTCTTTGATTTCGTTAACTGCTCGTTAACAGCAAAAATAGTATCCTTCAACTTCTGAATCTTCAGTTGGTTATCAAACGACTCCTTGGCTTCACCAATCACCTGGCGCGTGGTTTTAATCCGCTCCTTCACTTCCTTCTCCAATTGACGAACTACCGATTCATCAATCGGGATCGCATCATTGCTGAAAAGAGTCCTATATTGAGGCTTGCTCATCAAATCCGAGAAGTTTCGTGATAGTTCCGCAAAAATCCTAACGAGCTTCGAACTCTCAAGCTTGAGCTTTCGTGCATAAGAAGCATACGTAAGAGCCAAAGACTTATGATCATCGGCTTGCTTTCGGATCTGATCCAACCGAGGCTTAAGGGGATCCGTTTTCAACGCTAAAACAGATCTCCTTATCGATTCAAATCCATGCACAGAACCACCATCAGAATCCAAGTTATGCTGTTGATGAGAGGAATAAGAATGGGCAGTGAAAAGAAACGAGAGAGTGAGGAAAATGCAGAGAGCGATGAGTGTGAAGGGGAAGCTGAAGGGTCGGATCCTGGAGATAGGAAAACGAGGATTTACCATTTTCTTGTTTTGTTTTGGT
TARGET=202,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04347 Ps_contig_mira-and-tgicl-ass_franssen_32965
SEQUENCE=CACAAAGGAAGACGAGGAGGTCGTGAATTCCGTTGTAGAAGGCACAACACCATCCTACGCACTTGAATCGCGGCTAGGAGATTGTTTCCGTGCCGCCGCGATTCGTCGTGAGGCTTTACAGAGAACATCAGGGAAATCGTTGAAAGGCTTGCCGTTAGAAGGGTTTAATTATGACGCGATTTTAGGGCAGTGTTGTGAAATGCCGGTTGGGTTCATTCAAATTCCGGTTGGTGTTGCGGGGCCATTGTTGTTGGATGGGTTTGAGTATACAGTTCCGATGGCTACTACAGAAGGGTGTTTGGTTGCCAGTACTAACAGAGGGTGTAAAGCTATATATGCTTCTGGTGGTGCCGAAAGTGTTGTTTTGAGAGATGCTATGTCTAGAGCACCTGTGGTTAGGTTTTCATCAGCTAAAAGAGCTTCTCAGTTGAAATTCTTCTTGGAGGATCCTCTCAATTCTGAGACCTTGGCTGTTGTTTTCAACAGGTCAAGTAGATTTGCCAGGCTGCTAGGTATTCAGTGTGCTATGGCTGGGAAGAATCTTTATATGAGATTTACCTGCAGTACAGGTGATGCCATGGGGATGAACATGGTCTCCAAAGGGGTGCAGAACGTTCTTGATTTCCTTCGAAATGATTTCCCTGACATGGATGTTATTGGCATCTCTGGTAATTATTGTGCGGACAAGAAACCTGCTGCGGTTAATTGGATCGAGGGACGTGGGAAATCAGTTGTTTGTGAAGCAGTTATCAAAGAAGAAATAGTGAAGAAGGTATTGAAGACCAGTGTGGCTGCCCTGGTGGAACTCAACATGCTCAAAAATCTTGCTGGTTCTGCCATTGCCGGTGCTCTTGGTGGATTTAACGCCCATGCTAGCAACATTGTGTCTGCCATTTTTATAGCCACAGGTCAAGATCCGGCTCAAAACATTGAAAGTTCCCATTGCATAACAATGATGGAGGCCGTAAATGACGGGAAAGACCTCCATATCTCAGTGACCATGCCTTCCATTGAGGTGGGTACAGTAGGTGGTGGAACACAACTTGCATCTCAATCTGCTTGCTTGAATTTGCTTGGTGTGAAGGGTTCCAGCAAGGAATCACCAGGATCAAACTCGAGACTCTTGGCAACCATTGTTGCTGGATCTGTTTTAGCAGGCGAGCTGTCTCTGATGTCTGCCATTGCTGCAGGGCAACTAGTCAACAGTCACATGAAATATAACAGATCATGCAAAGATGTAACTAAAATATCATCTTGAGGTTGACGACATGGTTGCAGTCATTGTTTATGTTGTCTAAAATAAAATAAAAACATTAGGAGGGTCTCAGCGCAGAAACTGGAATTTAGGAAAGGAAAATAGGAGGCATGGGATTCATTGTTAATACCCATATATGGCTCTGTTGCTATGTGTAATGTTTTGTTATTTGGAGAGAGGAAACTAAAGTTCTACCTATTTGGTTATCCTCTCTAGATCCTTCCTCTCAGCCAAGCCACTGGTTGGGTGTTACCATTCTTGTTCTCCTTAACCAAGACATTGAAGTGTTGTATTATAATTCTTTTCCTGATATATATGTTTAGTATATAAATATGTATCATTGTTCTAAAACATATTTTTATTTTATGTAGTTGGGGACCAGTACCCAAYGAAATGAAAACC
TARGET=1296,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04348 Ps_contig_mira-and-tgicl-ass_franssen_32966
SEQUENCE=GGAGATTCATTTCTCATCAAACACAAAAAATGGCAATCACCATGGAAAAAAGCCTCTTCATCTTCCTTTTCATAGCCATAACCTCCATAGCTTCAACACAAGCAGGAAATTTCAACATCACAAACAAGTGCAACTACACCGTCTGGGCCGCCGCGGTCCCCGGCGGCGGTGTAAAACTCAACACCGGCGAATCATGGAACATGAACATCGCAAACGGCACAAACAACGCAAGAATCTGGGGTCGAACCAATTGCACTTTCGACAACTCCGGCGTCGGAAAGTGCCAAACAGGAGATTGTAACAACACTCTTGAATGTAAAACATTCGGAACACCACCGAACACAATCCTAGAATTCGCTTTGAACATGTACAACAACCTCGATTTCTACGACGTTTCGCTTGTACAAGGTTTTAACATCCCAATTAAGTTGACACCTTCGTCAAGCTCTTGTGGCATGGTTAATTGTACTAGAAATATTACCGATGAGTGTCCTGCACAACTTAAGGTACCAGGTGGATGTAACAATCCTTGTACAACGTTTGGAACATCTGAATATTGTTGTGACTTAAGTTCTGCTAAATCTGTTGCTACAAATGCTTCTGCCACGTGTACTCCAACAAATTATTCTAAGTTCTTTAAGGATAGGTGTCCTTCTGCTTATAGTTACCCTAAAGATGATGCAACCAGCACTTATACTTGTATGGGAGGAACTACCTATGATGTTGTGTTTTGTCCTTAAGTTATTTTTTTGAGGAATTTAATTTAATTTGTCTTGTTTTCATTTCATGATTCTTATTGTATACTTTGATATATGTATTTGTAATAATCAAATAAATGGGTTGTCTTAAAGGATTGATTCCTTTGCACATCTATGAGATATGATGTGTTATAATTAGATGTTTGTATAAGTAAATAAAAGTAAGGTTTATGTAAAAGTAAATTCTGATTATAAGAATAATTCATTGATGTTTAAATT
TARGET=756,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.2788
PRIMER_LEFT_PENALTY=0.081536
PRIMER_RIGHT_PENALTY=3.197255
PRIMER_LEFT_SEQUENCE=CTGTTGCTACAAATGCTTCTGC
PRIMER_RIGHT_SEQUENCE=GGAATCAATCCTTTAAGACAACCC
PRIMER_LEFT=582,22
PRIMER_RIGHT=860,24
PRIMER_LEFT_TM=60.082
PRIMER_RIGHT_TM=61.197
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=3.4227
PRIMER_LEFT_1_PENALTY=0.225403
PRIMER_RIGHT_1_PENALTY=3.197255
PRIMER_LEFT_1_SEQUENCE=CTAAAGATGATGCAACCAGCAC
PRIMER_RIGHT_1_SEQUENCE=GGAATCAATCCTTTAAGACAACCC
PRIMER_LEFT_1=669,22
PRIMER_RIGHT_1=860,24
PRIMER_LEFT_1_TM=59.775
PRIMER_RIGHT_1_TM=61.197
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=3.4227
PRIMER_LEFT_2_PENALTY=0.225403
PRIMER_RIGHT_2_PENALTY=3.197255
PRIMER_LEFT_2_SEQUENCE=AAGATGATGCAACCAGCACTTA
PRIMER_RIGHT_2_SEQUENCE=GGAATCAATCCTTTAAGACAACCC
PRIMER_LEFT_2=672,22
PRIMER_RIGHT_2=860,24
PRIMER_LEFT_2_TM=59.775
PRIMER_RIGHT_2_TM=61.197
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=3.4227
PRIMER_LEFT_3_PENALTY=0.225403
PRIMER_RIGHT_3_PENALTY=3.197255
PRIMER_LEFT_3_SEQUENCE=TAAAGATGATGCAACCAGCACT
PRIMER_RIGHT_3_SEQUENCE=GGAATCAATCCTTTAAGACAACCC
PRIMER_LEFT_3=670,22
PRIMER_RIGHT_3=860,24
PRIMER_LEFT_3_TM=59.775
PRIMER_RIGHT_3_TM=61.197
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=3.5698
PRIMER_LEFT_4_PENALTY=0.372557
PRIMER_RIGHT_4_PENALTY=3.197255
PRIMER_LEFT_4_SEQUENCE=ACCCTAAAGATGATGCAACCAG
PRIMER_RIGHT_4_SEQUENCE=GGAATCAATCCTTTAAGACAACCC
PRIMER_LEFT_4=666,22
PRIMER_RIGHT_4=860,24
PRIMER_LEFT_4_TM=60.373
PRIMER_RIGHT_4_TM=61.197
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_04349 Ps_contig_mira-and-tgicl-ass_franssen_32973
SEQUENCE=AAGGAAAAAGAATAACTTTGAGAATAGTATTACAGACACAGCATCCATCCATCTCAAAAGTCTCAACTGTTCCTTCAAGTCTTCAACCACTACACACTCACCACCACCACCGATGAAGAGCCACCAAACCTGGCCGTGTATCTCCTCTTCCTCTCGCTTCACCTCTCTCCCTCTCCCAAACCTAACCGCTTCCGAATTATCCGCCGCATATTCCTCCCGTTCCGTTCGTCTCTGTTCCCGTCCACCGCCGTCTAAACCTCCTCCATCCACCGCCGAAGAAACCGATTTCTCCTCCCTCCCGTACGACGTTCTGACGAGAATCGCAGCCTCCTTCGACCAGCCGAACCTCCAAGCGGCGTCGCTGGTATGTCGGTCATGGTGCGAGGCGTTACGGCCGTTAAGGGAGGCGATGGTGATGCTTATGTGGGGGAAACGGTTCAAGCACGGAAAGCGAGGGGTTTGTCGGAATACGGAGAAGGCACTTGATATGTTCACGAAAGCCGCTGCTCGTGGCTCCGCTCTTGCTATGGTGGATGCTGGTCTTATTTATTGGGAGAGAGGTGAGAAGATTAAGGCTCTTGATTTCTATCTCATGGCTGCTCATCTTGGAAATCCTTCTGCACAGTGTAATTTGGGGATTTCTTATCTTCAAGTTGAACCTCCAAACACTGAGCAAGCTTTGAAATGGTTATATAAAGCTTCCAAAGGTGGGAATGTCCGAGCCCAATACCAGCTTGCGCTTTGTCTTCACCGAGATGGTGGAAGTAGAAGTAATATAAGAGAAGCTGTTAAGTGGTATATGAAAGCTGCAGAAGGCGGGTATGTGCGTGCTATGTACAATACATCCTTATGCTACTCTTTAGGGGAAGGACTGACACGCAATCATCGAATAGCAAGAAAGTGGATGAAGCGGGCTGCCGATCGTGGTCATAGTAAAGCTCAATTTGAGCATGGACTTGCTCTTTATTCGGATGGAGACATGATAAAGGCTTTGGTGTACTTGGAACTTGCTAGCCGTGCTGGTAGAAAAGGGTGCCTTCCCATGTCAAGTAA
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04350 Ps_contig_mira-and-tgicl-ass_franssen_32973
SEQUENCE=AAGGAAAAAGAATAACTTTGAGAATAGTATTACAGACACAGCATCCATCCATCTCAAAAGTCTCAACTGTTCCTTCAAGTCTTCAACCACTACACACTCACCACCACCACCGATGAAGAGCCACCAAACCTGGCCGTGTATCTCCTCTTCCTCTCGCTTCACCTCTCTCCCTCTCCCAAACCTAACCGCTTCCGAATTATCCGCCGCATATTCCTCCCGTTCCGTTCGTCTCTGTTCCCGTCCACCGCCGTCTAAACCTCCTCCATCCACCGCCGAAGAAACCGATTTCTCCTCCCTCCCGTACGACGTTCTGACGAGAATCGCAGCCTCCTTCGACCAGCCGAACCTCCAAGCGGCGTCGCTGGTATGTCGGTCATGGTGCGAGGCGTTACGGCCGTTAAGGGAGGCGATGGTGATGCTTATGTGGGGGAAACGGTTCAAGCACGGAAAGCGAGGGGTTTGTCGGAATACGGAGAAGGCACTTGATATGTTCACGAAAGCCGCTGCTCGTGGCTCCGCTCTTGCTATGGTGGATGCTGGTCTTATTTATTGGGAGAGAGGTGAGAAGATTAAGGCTCTTGATTTCTATCTCATGGCTGCTCATCTTGGAAATCCTTCTGCACAGTGTAATTTGGGGATTTCTTATCTTCAAGTTGAACCTCCAAACACTGAGCAAGCTTTGAAATGGTTATATAAAGCTTCCAAAGGTGGGAATGTCCGAGCCCAATACCAGCTTGCGCTTTGTCTTCACCGAGATGGTGGAAGTAGAAGTAATATAAGAGAAGCTGTTAAGTGGTATATGAAAGCTGCAGAAGGCGGGTATGTGCGTGCTATGTACAATACATCCTTATGCTACTCTTTAGGGGAAGGACTGACACGCAATCATCGAATAGCAAGAAAGTGGATGAAGCGGGCTGCCGATCGTGGTCATAGTAAAGCTCAATTTGAGCATGGACTTGCTCTTTATTCGGATGGAGACATGATAAAGGCTTTGGTGTACTTGGAACTTGCTAGCCGTGCTGGTAGAAAAGGGTGCCTTCCCATGTCAAGTAA
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2462
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.214429
PRIMER_LEFT_SEQUENCE=AGCATCCATCCATCTCAAAAGT
PRIMER_RIGHT_SEQUENCE=ATAATTCGGAAGCGGTTAGGTT
PRIMER_LEFT=39,22
PRIMER_RIGHT=199,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.214
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=0.4107
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.378922
PRIMER_LEFT_1_SEQUENCE=AGCATCCATCCATCTCAAAAGT
PRIMER_RIGHT_1_SEQUENCE=AGCGAGAGGAAGAGGAGATACA
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=157,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=59.621
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=119
PRIMER_PAIR_PENALTY_2=0.4264
PRIMER_LEFT_2_PENALTY=0.031769
PRIMER_RIGHT_2_PENALTY=0.394672
PRIMER_LEFT_2_SEQUENCE=AGCATCCATCCATCTCAAAAGT
PRIMER_RIGHT_2_SEQUENCE=CGAGAGGAAGAGGAGATACACG
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=155,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=60.395
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=0.5012
PRIMER_LEFT_3_PENALTY=0.031769
PRIMER_RIGHT_3_PENALTY=0.469469
PRIMER_LEFT_3_SEQUENCE=AGCATCCATCCATCTCAAAAGT
PRIMER_RIGHT_3_SEQUENCE=GTTAGGTTTGGGAGAGGGAGAG
PRIMER_LEFT_3=39,22
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=59.968
PRIMER_RIGHT_3_TM=60.469
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=0.5428
PRIMER_LEFT_4_PENALTY=0.031769
PRIMER_RIGHT_4_PENALTY=0.511039
PRIMER_LEFT_4_SEQUENCE=AGCATCCATCCATCTCAAAAGT
PRIMER_RIGHT_4_SEQUENCE=GCGAGAGGAAGAGGAGATACAC
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=156,22
PRIMER_LEFT_4_TM=59.968
PRIMER_RIGHT_4_TM=59.489
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_04351 Ps_contig_mira-and-tgicl-ass_franssen_32983
SEQUENCE=ACGTTCGGGGGGTAATCAAACTTTGACGATTCAGAACTGTGTGACAAAGAGACTCCGAAAGAGTGAAACGGAAAAATGGGAAGGAAGAAAGGGTATGTCGAATTCGAGGAATCTCCACCGGACGATTTTGATCCGGCGAATCCTTACAAGGATCCGGTGGCTATGTTGGAGATGAGGGAACACATCGTCAGAGAAAAATGGATCCAAATTGAAAAGGCTAAGATTATTAGGGAAAAGCTTCGTTGGTGTTATCGCATTGAAGGTATTAATCATCTTCAGAAATGTCGTCATCTTGTTCAACAGTATCTTGAGTCTACACGTGGTATTGGTTGGGGCAAGGACGGTCGTCACCCTTCTCTCCATGGACCAAAAGTTGAGCCAGTTGAGTCCGAATAATTTCCCCATGGATAAATAAAAGGGAAGTTTTATTACCCAAATGATGACATTGCCGAAATCAGATTGTAGGTGGAGGAATGCCTATTGTTCAAGCTGTTGTGTTTTGTTAATTTTCCATAGTCAGATTTCCTGTGAACTACAATTGTATTTCGCCTTGAGCAAAAGTACATGAGATTGTGAAATAAGTTTCTTTGTTCTTTGCTATATGATTGTTGCGTGCTTCAACAATTGAATGTGTTCTCTACTGCATTCCTAATATTTAACATGACATACCTTTATTAATTTTAAAACCTCACCATTTCCNAGTYATGATGATGATGGTGTCACTACCTATGTGGTNCATGCATAGGATTATTACGTTTTGGATTAGTTTAATTAAATTATATTCTAATTTTAAAAA
TARGET=705,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04352 Ps_contig_mira-and-tgicl-ass_franssen_32984
SEQUENCE=ACAAGAAATTAGTTTGAAGATTTATTNTAGAAATTGCATCTTCTAATGCATTCATAATGCAGACACTCAACGCATATGCAAGTCAAAATATGGTTAGAGTATAGCTTGTATCCAAAGATTCAACAAAGAAAAAACAACTTAATTTTTCACTCCATTTGTAATATTTGCAATTTTATCTAGTATGACACCAAACATGTATTTGGATCATACCCTTACTTTCTGAGCAGCTTCCTTGATCTGCTCAATTGTAAAAACACCAAAGAAGTCGTCATCTGTGACACCGTTGATAACTGCCAACGCGACATCGTCAACACTAACCGGTGGAGCCAAAAGTAGATCAGAAGCAGGGAGTGAGCTTAATGGTTTTGTGAAGTTCTCTATTGCTTTTAGAATTCTTTCAGCTGGCTCCCCTACTAAATCCAAAGGAATCTCGAAACCGTCCACTTTTCTTTTCCCGTATATGAAACCTGGTCTTAACACAATACCTGAATTAGGGAATTTCGATAGAACCTCAGATTCTGCTTTCCTCTTTCCTGTGAAGTACCCCGATGATAGTAAAAACGATGGCAAGTTATATTCATGAACTGAGATCAATATGAATTTGGGAACTCCATATTCCTTTGCAGTATTCACAGCCACAACATTAGCCTCGCCATTAATCTTACTCATTTGTTCGTCACTACCAAAACCTCCAAGTGTCGAGACAACCGCAGTAGCTCCAGGAAGTACTTCGTCCCAGTTTACATAAAAAACGTCTCCTGAAATCCAAGTAACCTGATCTATCCACGAATCCGAGTAAGAAGGACGTCCCGACCTGTTTACGCTTATGACTTCTATGCCCTTGGAAACCGCTGCCTTGCATATGGCAGAACCAACAAACCCATTGCCTCCCAATACTACAATCTTTTCACTTTTAACATCAGCCACAACATCTATAGTTGATGTTGAAGATTCAAAACCACCAACACCTGAATAACTGCAGCTAACTGCAAACCGGTGATTTTTGAAATGCAAATTGGGTGAAGTGTTGATTAAAGGTCGTTTGAGAGAGAAAGCGGTGGGAATGAGACGAGAGGAAACTGCGGGGAGAGACAAAAACGACGCCATTTTGGTGGGTTGAATCTAGGCACTTAGTTCGTGAAGATGCAATAAACAGAGAATTTTGAGAAGGAAGAAGAAGAAGAAACAGTGACGGTTTGAAACCCCCG
TARGET=1171,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04353 Ps_contig_mira-and-tgicl-ass_franssen_32985
SEQUENCE=ACAGTGGTATCTAACGCAGAGTATTCTCCTTTTGTTTTGTTTTGTGAACTTTCTCTTTCTTCGTTTCTTATCCTCACCCGCGAAAACTAACGAATTCTCACTCCTTTAATCACCGTTCATCATCGTCCTCCTCTTCCGCCGCGGTAAGATCGGAAAATCAGCTCGTCAATTGGGATTTGTTGGGAAAAAAAGAGCCTAGGAGTTGGGCCTTGGGTATGTTAAGATTTTGTTTGGGCTGTCCATCCATTGAGTCCAAGGAAAAAGCCTAGAACAATGCCCTTCTCTTTCTCTATCTCACAACCAACTGCTGCAAATGCAATGCGCATACCAGCAACAATGATGTCAACCAATTCCCTTTTTTCATGTTCCTTAAGATTATTCACAACCAAATCCCCACTTTCCCTCTTCAACAAAAACAACTCTACTTTCTTCATTAAACCCACAAACACCCTCTTCTCTTTCAAACCCATCACTTTCCAAAGACTCCCAACAAAACCATCATCTTCATCGTCACCACCGCCATCCACTTCCCTTCAACCCATCGAAGAACTCCCTCCAAAGCTTCAAGAAATTGTAAACCTCTTCCAATCCGTACAAGAACCCAAAGCCAAATACGAACAGCTTCTTTTCTACGGAAAAACCCTCAAACCCCTCGAACCCCAATTTAAAACTAAAGAGAACAAAGTTGAAGGTTGTGTTTCTCAGGTTTGGGTCCGAGCCTACATGGACAGTGACAAAAATGTCGTCTATGAAGCTGATTCAGATTCTGTTCTCACCAAAGGCCTCGCCGCCTTATTAGTTCAAGGCTTTTCAGGTCGACCCGTTAATGAAATTATCCGGGTCACACCAGATTTTGTAATGCTTCTTGGATTGCAACAGAGTTTAACCCCTTCTAGAAATAATGGGTTTTTGAATATGCTCAAGTTAATGCAGAAAAAAGCACTTAGGCTCTATGTGGAAGCTGAAAAGGGTACTTCTGAATTTAACTCAATTGGAAATTCTGATTTCAAAGATGATAACTTTGTTGAGAATTCAAGTGGCCCTTCTGTAACCCCAGGTTTAGGTGTTGGTTTTAGTTCTAAGGTTGATGATGTGAATGTTGAATTGGAAGGGAGAGGGAAGAGGATAAAGGAAAAGCTTGAAAAGGACCTTCATCCTATTGAGTTGGAAGTTGAAGACGTGTCTTATCAACATGCTGGGCATGCTGGTGTTAGAGGGAGCAATGGTGAAACACATTTCAATGTTAAAGTTGTTTCTGAAGAGTTTCAAGGGAAGAGTTTGGTTAAGAGGCATAGGCTTATTTATAACTTGCTTCAAGATGAGTTGGAGTCTGGACTTCATGCGTTGTCTATTGTGGCCAAGACACCTTCTGAAGTCGGTGAAGGATGAAAATTTTAATGCTCCAAAAGAGAGGTCAAAGTTTTCTCCTTTGTCTCTGTTGTGTTTGAGAATGTACTTGTGTTGTTTGGTGAATCATAAGGTTATTTATATTTGTTAGATTACTTGTTTTGGTTTAAATATATTGTCTTGTGACATTACACATTCATTTTTCTGATAGCATTTCCAATAATTTGAGGGATTCTGTAAATTTATAGAGTTCAATGGTATGAATTCCAAAGATATTTCGACATTGAAAATCAGAAGGGTTTTTTGTGGCTTCTAAACAATATTGTGAGAATGTCTTATTGTTTTGGCCATTTTATTTAAATAAGGATTCAAGGGTAAG
TARGET=30,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=5.4466
PRIMER_LEFT_PENALTY=5.365487
PRIMER_RIGHT_PENALTY=0.081111
PRIMER_LEFT_SEQUENCE=ACAGTGGTATCTAACGCAGAGTATT
PRIMER_RIGHT_SEQUENCE=GAAGAGGAGGACGATGATGAAC
PRIMER_LEFT=0,25
PRIMER_RIGHT=135,22
PRIMER_LEFT_TM=57.635
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=5.4793
PRIMER_LEFT_1_PENALTY=5.365487
PRIMER_RIGHT_1_PENALTY=0.113803
PRIMER_LEFT_1_SEQUENCE=ACAGTGGTATCTAACGCAGAGTATT
PRIMER_RIGHT_1_SEQUENCE=CTAGGCTTTTTCCTTGGACTCA
PRIMER_LEFT_1=0,25
PRIMER_RIGHT_1=268,22
PRIMER_LEFT_1_TM=57.635
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=5.5895
PRIMER_LEFT_2_PENALTY=5.365487
PRIMER_RIGHT_2_PENALTY=0.224014
PRIMER_LEFT_2_SEQUENCE=ACAGTGGTATCTAACGCAGAGTATT
PRIMER_RIGHT_2_SEQUENCE=CGAGCTGATTTTCCGATCTTAC
PRIMER_LEFT_2=0,25
PRIMER_RIGHT_2=164,22
PRIMER_LEFT_2_TM=57.635
PRIMER_RIGHT_2_TM=60.224
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=5.5895
PRIMER_LEFT_3_PENALTY=5.365487
PRIMER_RIGHT_3_PENALTY=0.224014
PRIMER_LEFT_3_SEQUENCE=ACAGTGGTATCTAACGCAGAGTATT
PRIMER_RIGHT_3_SEQUENCE=ACGAGCTGATTTTCCGATCTTA
PRIMER_LEFT_3=0,25
PRIMER_RIGHT_3=165,22
PRIMER_LEFT_3_TM=57.635
PRIMER_RIGHT_3_TM=60.224
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=5.5950
PRIMER_LEFT_4_PENALTY=5.365487
PRIMER_RIGHT_4_PENALTY=0.229480
PRIMER_LEFT_4_SEQUENCE=ACAGTGGTATCTAACGCAGAGTATT
PRIMER_RIGHT_4_SEQUENCE=TAGGCTTTTTCCTTGGACTCAA
PRIMER_LEFT_4=0,25
PRIMER_RIGHT_4=267,22
PRIMER_LEFT_4_TM=57.635
PRIMER_RIGHT_4_TM=60.229
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_04354 Ps_contig_mira-and-tgicl-ass_franssen_32996
SEQUENCE=AAAAGGTTTAATTATTTTCACTCAATTGCTTTGTCCATCGTACTGCTTCGGTAATTATATAATCAAACTGAAATTATATGGTAATTATTCTCCCCAAAATTGTATCACCCAAAAATGTCTTATGATCCCAATGCTAATGATATACTAACAGTTAACAACAACAAAAAAAGGTAAACTGTCCAACATCTATCAATAGGAATATAGTACCATGTCATTCCATTGAACACAAACGGTGCATTGGATGCTGACAGAAATGTAAAATTACTTCAGAAGAAAACAAAGCTTGTAGCTTAAAAACCATTACATGGCATAACTTGAAATGCGAACCTCCAGCAAGACATTCGGAATAAGAGAACTAATATGTGACATTGTTTGGCAAGTTCCGGATGACATTACTCTTCATCCCCATCTTGCTACGCATATGCTCCACACCCGATTTGTTCATCTGCTTCAACACTTTCATTCTCACAAGTTGCTCCCTTGATTGGACAGTGGCCAAACCCATGCTCCTGTACCTTGAAGCCAATTCAGCAGTAATGGCAATGTTGTTAGCTGGAGATGGCCTTGGTTTCTGACGATAATAACGCAAATATTCGCGGGAACCAAGAGTTCTGGTCGATTTTCTATCACCAGACATCTTAGTGATTACGAGCTCAGACCCACCACTAAGTTCTATATTATTATCAGCATCACCGGATGCAACTAGCTGCTTCCCTTGATCATCAACATAACTGCTGGTATAATCATAGAATTCTTCCAGCTCTACTTCCTCCTCCTCATCATCATCACCATAATGCACTTTACAGTGATTTTTTGCAACCATATGTTTCCTGACTGCTTCCAAGCTGCTAAAAGGATGACACCTATCGTTACAGTACAGACACAAGTAGTCCCTTTTGACCTTTAGACCAAGATAAGTGAGGAGCCCCTTTGGATCTTTCAAATACTCAACGTCAGGAATGAAGAATCCATGATGCTTGTGCATGTGAACCATGCAGTTTTCAATTGTTTTGTGCTTGTGATCACACATAAAGCAACAAGATGGATCTATGTCCACAACATCATCTTCATCCATATCAGCATTCTCATCCATGTTCAAATCTGCCAAGGACTTCTCAGCATCATCCATCAGATCATCAGAATCAACCTCCTCCCATTCATCTTCACTGTCTTCATCTTCAACAGAGTCATCTACCAATCTTCTAGGAGGCGGCTTATTCACAACGCGTGGTGGAAGTGGCTTAATTATAGCTTTCCCATCTGAATCACTAGCTCCTTCAGAAGCCCGAACCATGTGAGTTCTCGACTTCAGATGCTCCGCATGAGCCTTAGCACTTTTATACCCCTTGCCACATAGGCCACAGCTATAGAGCATAGGGGTTTCATTAGATTTATCTCTCTCTTGAGCAAGTGCTGATTGTCTAGCTAGAAACAAAGCTTCTGTCACTCCAGGAACCCCAGCCACCTTGCGTTTGAGATTGTAGCGATGCCACTCGGATTTGTAATGGAGTTTTTGGTCGGAATCATGATTGAATTCTGTGTTGCAGGAATTGCAGGTTAATCCTGACATAGTTTGGAGAGAAAAAGATGCAGATGAAGAGAAAGAAACGTGAGAAGAGAATTGTAGAAGCAACAGAAATAAAATGGTTATTCTGTCGGCCGAAAAGACAAT
TARGET=777,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2007
PRIMER_LEFT_PENALTY=0.174518
PRIMER_RIGHT_PENALTY=0.026163
PRIMER_LEFT_SEQUENCE=GCTCAGACCCACCACTAAGTTC
PRIMER_RIGHT_SEQUENCE=TTGGTCTAAAGGTCAAAAGGGA
PRIMER_LEFT=651,22
PRIMER_RIGHT=911,22
PRIMER_LEFT_TM=60.175
PRIMER_RIGHT_TM=59.974
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.3204
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.210886
PRIMER_LEFT_1_SEQUENCE=CCAAGAGTTCTGGTCGATTTTC
PRIMER_RIGHT_1_SEQUENCE=GGTGTCATCCTTTTAGCAGCTT
PRIMER_LEFT_1=602,22
PRIMER_RIGHT_1=863,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=59.789
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.3630
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.253482
PRIMER_LEFT_2_SEQUENCE=CCAAGAGTTCTGGTCGATTTTC
PRIMER_RIGHT_2_SEQUENCE=TTGGAAGCAGTCAGGAAACATA
PRIMER_LEFT_2=602,22
PRIMER_RIGHT_2=843,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.747
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.3854
PRIMER_LEFT_3_PENALTY=0.174518
PRIMER_RIGHT_3_PENALTY=0.210886
PRIMER_LEFT_3_SEQUENCE=GCTCAGACCCACCACTAAGTTC
PRIMER_RIGHT_3_SEQUENCE=GGTGTCATCCTTTTAGCAGCTT
PRIMER_LEFT_3=651,22
PRIMER_RIGHT_3=863,22
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=59.789
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.4280
PRIMER_LEFT_4_PENALTY=0.174518
PRIMER_RIGHT_4_PENALTY=0.253482
PRIMER_LEFT_4_SEQUENCE=GCTCAGACCCACCACTAAGTTC
PRIMER_RIGHT_4_SEQUENCE=TTGGAAGCAGTCAGGAAACATA
PRIMER_LEFT_4=651,22
PRIMER_RIGHT_4=843,22
PRIMER_LEFT_4_TM=60.175
PRIMER_RIGHT_4_TM=59.747
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_04355 Ps_contig_mira-and-tgicl-ass_franssen_32999
SEQUENCE=CGGGGTACTAACAAACCAAATTGTGCAAAACAACGAGGAAGAAGAAGAAGAAGCAAGAACATATAAAAATGGCAGAGAAATTGGCACCGGAAAAGCGTCACGTTTTCCTCCACAACGGTCAAAAGGTTTTCGAATGGGATCAAACCCTAGAAGAGGTCGATATTTACATTAATCTTCCTCCTAATGTTCATTCCAAGCTATTTTACTGTAAGATTCAGTCCAAACACATTGAACTTGGCATCAAAGGCAATCCTCCATTTCTCAATCATGATCTTAGTTCACCGGTGAAGACAGATTCTTCGTTCTGGACTTTAGAGGATGACATAATGCATATAACATTGAATAAAAGAGATAAGGGTCAGACATGGGCTTCTCCTATATTAGGTCAAGGTCAGTTGGATGCTTACTCTAGTGATCTTGAGCAGAAACGCCTCATGCTTCAAAGATTTCAAGAGGAGAACCCGGGTTTTGATTTTTCACAAGCTCAATTTAGTGGAAACTGCCCTGATCCAAGGACCTTCATGGGTGGAATTCGAACAGATTGATATTTGCCACATGAGTTTGTATTTACCTGCGGTAATGGAAGATGATAATCAGATTAATAATCTGTGTAATCTCTTGTCTCCTTGTTTGGTGCCATTTAGTTTAATTAAAAAACAATCTTAACATACTGTAATGAATAACTGACTTTTGGCATCTTGCGGTAGTATTTATTAATCTTTCAACTGCCCTTTGTTGGCTTTTAAAATTAAATATGTATATAGAAAAGGCATTTGAGTAGGATTTATATTAGACATCATTGTTCTAATATGATATACTATGTAACTATTTTGATTTGAAATCTCATGGATTACCAAGCCAGATTAAATCAAGCTAGTATGGAACATTGTGTCAT
TARGET=38,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5413
PRIMER_LEFT_PENALTY=2.007073
PRIMER_RIGHT_PENALTY=0.534252
PRIMER_LEFT_SEQUENCE=CCAAATTGTGCAAAACAACG
PRIMER_RIGHT_SEQUENCE=CTTTGATGCCAAGTTCAATGTG
PRIMER_LEFT=15,20
PRIMER_RIGHT=245,22
PRIMER_LEFT_TM=60.007
PRIMER_RIGHT_TM=60.534
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=2.6586
PRIMER_LEFT_1_PENALTY=2.624537
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=CAAATTGTGCAAAACAACGAGG
PRIMER_RIGHT_1_SEQUENCE=GCTTGGAATGAACATTAGGAGG
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=62.625
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=2.6586
PRIMER_LEFT_2_PENALTY=2.624537
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=CCAAATTGTGCAAAACAACGAG
PRIMER_RIGHT_2_SEQUENCE=GCTTGGAATGAACATTAGGAGG
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=62.625
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=2.6678
PRIMER_LEFT_3_PENALTY=2.007073
PRIMER_RIGHT_3_PENALTY=0.660713
PRIMER_LEFT_3_SEQUENCE=CCAAATTGTGCAAAACAACG
PRIMER_RIGHT_3_SEQUENCE=AACGAAGAATCTGTCTTCACCG
PRIMER_LEFT_3=15,20
PRIMER_RIGHT_3=303,22
PRIMER_LEFT_3_TM=60.007
PRIMER_RIGHT_3_TM=60.661
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=2.7707
PRIMER_LEFT_4_PENALTY=2.007073
PRIMER_RIGHT_4_PENALTY=0.763661
PRIMER_LEFT_4_SEQUENCE=CCAAATTGTGCAAAACAACG
PRIMER_RIGHT_4_SEQUENCE=TCGACCTCTTCTAGGGTTTGAT
PRIMER_LEFT_4=15,20
PRIMER_RIGHT_4=159,22
PRIMER_LEFT_4_TM=60.007
PRIMER_RIGHT_4_TM=59.236
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_04356 Ps_contig_mira-and-tgicl-ass_franssen_33001
SEQUENCE=GCGGGGATATCTCTCCAACTTTCCTTTCTAACACAATGTCACGAAAACCAATGGCCACCTCCACCCTTTCACCTTCCCCTCTCCTCCTCCTCCTCCTCCTTTTCCACCTCGTCGCCACCGTCACCTCCATCGGCGTCAACTACGGCACCCTCGGAGACAATCTCCCACCTCCAGCAACCGTAGCAAATTTCTTAAAAACAAACACAATCATCGACAGTGTCAAGATCTTCGACGTTAGTCCTCAGATCCTTCAGGCTTTCGCGAACACCGGCATCTCCGTCACTGTGACGGCACCCAACGGTGATATCGAGGCGTTGGCGAAGATTGATTCGGCTCGTCAATGGGTTGTTACACATATTAAACCTTTTCATCCTCAGACGAAGATTAATTATATTCTTGTCGGAAGTGAAGTTTTTCATTGGGGTGATAGTGTTATGATTCGGAACCTTGTCCCTGCTATGAGAACGCTTCATTCTGCTCTTCTTGCTGAGGGTATTACCGACATTAAGGTCACTACAGCACATTCCTTGGCAATATTGCGACAATCATTACCACCAAGTGCAGGGCAATTCAGACCAGGATACGCAAAATACTTTATAGGACCAATGTTGAAATTCCTAAGACAAACAAGAACACCGTTTATGGTAAATCCATATCCATATTTCGGTTACAATCCGAAAAACGCCAACTTCGCATTGTTCAGACCAAACCGTGGCTTATTCGACAGGAACACAAAACTACTATACACAAACCAATTCGACGCTTTAATGGACGCGGTTCATTCGGCAATGAAGGCTCTTGGATATGGTGATGTTGATATCGCTGTTGGTGAAACGGGTTGGCCTTCTGTTTGTGATGGTTGGGATGCTTGTAGTGTGGCTAATGCTCAGAGTTATAATGGTCAGTTGATTAGGCATTTGGCGGAAGGGAAAGGGACGCCATTAATGCCGAATAGACGGTTTGAGACTTTTATTTTTGCCTTGTTTAATGAGAATCAGAAACCTGGTCCTATTGCTGAACGTAATTGGGGACTCTTTCAACCTGATTTCTCTTCGGTTTATGATGCTGGAATCCTGCGTAACGGACAGAAACCAGTAGCACCGGTAAAAGGAGGAGGAAAAATGCCAACGCCGCGTCCGGTAGTTGGAGGGCAGAAATGGTGTGTGCCAAAGGCGGATGCGAGCCCTGGAGCATTACAAGCGAATATAAACTATGTTTGCAGCCAAGGAATTGATTGCAGGCCGATCCAACCGGGAGGAGTTTGTTATGCTGCCAACAATGTTAAGGCAATTGCCACGTATGCGATGAATGCTTACTATCAGGCTAATGGAAAACATGATTACAATTGCGACTTCTCGCACTCCGGTGTTACTACTTCAGTTAATCCAAGTCATGATAATTGTAGAATCTGAGGATGTTTGGAGATAGAAAAAGGCCGTTGGTATTGGCATTGGCATATTAGAGGGAAAATGCAGTTGATGTGGCTTTGAGTTAGTAGTGACTATGCATTCATTTACTTGGTTATTTAGTTTGTATATTATATTATGGAAAATTGATGTTAACGTTGGCATGACATTTCATGCTTTGATTAAGTATATGTAATGTGTTTCGGTGATTCCTGATTTCAAAAGCATTTGATGCTTCCATTTTGTNRAAATCAATANTTTTATGAATTA
TARGET=81,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04357 Ps_contig_mira-and-tgicl-ass_franssen_33003
SEQUENCE=GAACAAACTCCATCTTCTTCCTTGCAGTTACACTNGTGATACTCTAACCTCCAACCACTCCTACAAAAAAAAAATGACTCTATTTTCTTCACTGCAAATCACCACCACCGTCTCTCTTCACTCTTTCAATCCCTCCTCAACCATCTTCCACAAATCCTTTCTTCCTTGTTTCGCAACTTTACCATCACTTTCTCTCAATAGATTCGTCTTTTCCACTCAAAAATCTCGAACAACAACAACTACTACTACCACCCGTGCTTCTTTTCATGAAAATCCAGTTCTATGGGTTGGTAGAATCTGCGTCTTCTACGCTCTTCTCAAGACTGGATTAGTCGGATCTCCATCAAATCCGTTCCTCTCAGATTTGGAAATTGGTAGTAATGGTGATAGTAATGATTCTGGTGACTTAGGGTTCTCTAAATGGACACAGTCCATACTTGGAAAACCAGCCAAGGAAGAAGCTAACGGAGGGAACCTTACGAGCAAATGGCATCCTACGACAAAGGGTACACTGAGGAGGAACTACAGAGTCCCATCCAAGTCTGAGGGGCGACGACTTCTCAAAGCCATTGCATCTCTGTTGTCAGATGATGACCACTTTGTTGATGCCACTTCTCACAAGGGTTGCCAAATCCGGCGAGAAAGTGCCCATGGAGAAAGTGTCTGTTGCAACAACGTGAGAGCTCTTTTTGATGAGCTACCAACTCCTCATCTCATTGTGGAGATCACTCCTTTTCCTGTTGGACCTCTTAGTGACAAGGATTACACTAAAGCTGAAAAGCTTGAGAAGGTTCTCAGATCTAGCCCTTCTGTTTGAACCCTCCTTGATGTTTCTCGTTTTCATGTATATATTCTTTGCTAGCTTGTAAAATATTGGCACCACGRAGAGNAATTTGTATAACATNTTTTACATTTGAGTTGAATCATAATCACATCGTTCAAA
TARGET=229,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04358 Ps_contig_mira-and-tgicl-ass_franssen_33005
SEQUENCE=AGAATAGAGAGGATCTCAACTTTAAACAAGTGATTAAACTACAATAAATTCATAAGCACACTACATGTTAATAATTTTCTTTACATTTTACACAATTTAAGTGACTATGAGGCAACAAAACAAAATTGATTCCACCTATAAATCCGAAGGTAACGACGATTCACTTTCTGGCTTCTGTGGTGGTGGCACAGTTGCAGCAGTTTCATTAGCAGGAGGCTGGATTTGCTGTTCGATTTCATCTTCTTTAGGAGAGGAAGTTTGCTCCTGCTGTTGTGCAGCAACTGCCTTTAGTTGCTCCTCTGTAATCTTAAGATACTCCTCAGAACTGTATGCTTTACTTTCATCTACCTTCCCATTAGGGTCAACAGCAGTCTGAGAATTATTGGTAATTTCAGTGGCTGATGGAGTTGGCGTGGTGTTGCGTACATTGGAAGAGTATGTGCTTTGTAATGGGTTTGTAATGTCATCAATACCAATCTCCCGTTCGAGCGTACTCTTAAATTCCCTTGAAACATCCTGGATTTCTCTAATGGTGGGTTGAAATTCACGCAATGTTTTTCCCAGATTTCGAGCAACCTCAGCAAGACCTTTAGGACCAAAAACCAACAAAGCCACAACCCCAATTACCAAAGCTTCAGGTGCTCCAACCCCAAAGAGAGACGCATAAACAACCTTACCCTTACACCGACCCTTTTTACCTATAAAATGAAAAAGAGGTTTTTTGGGTTTAGTTGAGAAACCCAATTGCTTGAAACCATTCCAAGGTGTGAAAAGGCGTTTACCCAATGAATAGAGATGGAAATGTTGGATTTTTGAATGTGAAGTTGGTGTACAAGTGGACAGAGACATTGAGCATGTTCCTAGTTTGGGACATAACAACATGGTTGAAGTTGAAGAAGCAATTGCCAGAGATGGTGTCATTTATGAATAATGGTTGGATTTTGGTTCGTCCTAGAATCCTATCTCTAATCTAATCTAATGGCTAAATCAAAATTCCCCGAC
TARGET=965,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2128
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=1.199411
PRIMER_LEFT_SEQUENCE=GAGAAACCCAATTGCTTGAAAC
PRIMER_RIGHT_SEQUENCE=GTCGGGGAATTTTGATTTAGC
PRIMER_LEFT=733,22
PRIMER_RIGHT=1001,21
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=59.801
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=269
PRIMER_PAIR_PENALTY_1=1.2854
PRIMER_LEFT_1_PENALTY=0.085951
PRIMER_RIGHT_1_PENALTY=1.199411
PRIMER_LEFT_1_SEQUENCE=AAGTGGACAGAGACATTGAGCA
PRIMER_RIGHT_1_SEQUENCE=GTCGGGGAATTTTGATTTAGC
PRIMER_LEFT_1=833,22
PRIMER_RIGHT_1=1001,21
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=59.801
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=1.2854
PRIMER_LEFT_2_PENALTY=0.085951
PRIMER_RIGHT_2_PENALTY=1.199411
PRIMER_LEFT_2_SEQUENCE=CAAGTGGACAGAGACATTGAGC
PRIMER_RIGHT_2_SEQUENCE=GTCGGGGAATTTTGATTTAGC
PRIMER_LEFT_2=832,22
PRIMER_RIGHT_2=1001,21
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=59.801
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=1.3355
PRIMER_LEFT_3_PENALTY=0.136083
PRIMER_RIGHT_3_PENALTY=1.199411
PRIMER_LEFT_3_SEQUENCE=GGCGTTTACCCAATGAATAGAG
PRIMER_RIGHT_3_SEQUENCE=GTCGGGGAATTTTGATTTAGC
PRIMER_LEFT_3=773,22
PRIMER_RIGHT_3=1001,21
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.801
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=1.3355
PRIMER_LEFT_4_PENALTY=0.136083
PRIMER_RIGHT_4_PENALTY=1.199411
PRIMER_LEFT_4_SEQUENCE=AGGCGTTTACCCAATGAATAGA
PRIMER_RIGHT_4_SEQUENCE=GTCGGGGAATTTTGATTTAGC
PRIMER_LEFT_4=772,22
PRIMER_RIGHT_4=1001,21
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=59.801
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_04359 Ps_contig_mira-and-tgicl-ass_franssen_33006
SEQUENCE=CGACAGAGTACGCGGGACATCCTCTCTATATATGCATTTTTTTTCTTTCCTCTTAATTTTATTATTATTATTATTGGAAAAAAATTGAGTAGTGTACTTTGTGAAGAGCAACAAAAGCTTCTTCTCAATCTCAAAAAATCTCTCACTATGAGAGACAACATTATCGACAACATCCCACCAAGAAAGCTCCTCACCCGCCGACAATCCACCAACACCTCCGCCGACATGTTTTCCGAGGAAGATCCTTATTCCTCCGACCACTTCCGCATGTACGAGTTCAAAATCCGGCGATGCACACGTAGCCGCAGCCACGACTGGACTGACTGTCCCTTCGCTCACCCTGGCGAAAAGGCTCGCCGCCGTGACCCTCTCCGGTTTCAATACTCCGGTGAAGTTTGCCCTGATTACCGCCGCGGAAACTGCGAACGCGGCGACGCGTGTGAATTCTCGCACGGCGTTTTTGAATGCTGGTTGCACCCTTCGAGATACAGAACTGAAGCTTGTAAAGATGGTAAGAATTGTAAGCGGAAAATTTGCTTTTTTGCTCATACACCCCGTCAACTCAGGGTTTTGCTTTTGCCACCCCCTCCTTCTCCACCTCCTCAGAACAACGTTAAAAACTGTTGTTCTTTTTGTCATTGCTGTTCTAATTCTTCTTCTTCGTCACCCACTTCTACTTTGTTGAGTGGACCTTATTTTTCTTCTTCGAATTCACCACCTTTGTCACCTTTGTCCAATTCAAATTCCAAAGATGTGCATGTTCTTAACGAGCTTATTCGATCTATGGAAAGTTTCAATTTTGGTGGTGATGATTCTCCTGTTTCTGCTTTGGGCGGTGGAAAATATTATAATTCAAGTGGTAGTAGCAAGTTTTTGAGGGAAGAACAAAGGATTAACGTTGTTGAGGATGATGTCAGTGCCAACCCAGATTTTGGGTGGGTGAATGAGTTGCTCATGTAGATGTTGATGATGATGGTGGTGATGTCAGTGCTGTTGTGGATATGATGATGGTGATGAGATGATGATGAATGGATGGTGATTTGATTAAGAAAATTAAGTGTAGATATAGTAATGTGATCATGTGAGTTATATGATCCTTAATTGGTTTGCTATGTCTCTATTTTTAATTGTAATTCAATTCGATCTTGTAATTTTTCAGTTTATTACTTTCTGAATTTGTACGTTTGTGAAAATTATATATTGTGTAATTTGTATTTCCATTAATAAAATAGATTTTATGCCTA
TARGET=59,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7840
PRIMER_LEFT_PENALTY=1.715693
PRIMER_RIGHT_PENALTY=0.068302
PRIMER_LEFT_SEQUENCE=GTACGCGGGACATCCTCTCTAT
PRIMER_RIGHT_SEQUENCE=TAAGGATCTTCCTCGGAAAACA
PRIMER_LEFT=7,22
PRIMER_RIGHT=247,22
PRIMER_LEFT_TM=61.716
PRIMER_RIGHT_TM=60.068
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=1.9182
PRIMER_LEFT_1_PENALTY=1.715693
PRIMER_RIGHT_1_PENALTY=0.202525
PRIMER_LEFT_1_SEQUENCE=GTACGCGGGACATCCTCTCTAT
PRIMER_RIGHT_1_SEQUENCE=AAGTGGTCGGAGGAATAAGGAT
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=262,22
PRIMER_LEFT_1_TM=61.716
PRIMER_RIGHT_1_TM=60.203
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=1.9931
PRIMER_LEFT_2_PENALTY=1.715693
PRIMER_RIGHT_2_PENALTY=0.277414
PRIMER_LEFT_2_SEQUENCE=GTACGCGGGACATCCTCTCTAT
PRIMER_RIGHT_2_SEQUENCE=GGTCGGAGGAATAAGGATCTTC
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=258,22
PRIMER_LEFT_2_TM=61.716
PRIMER_RIGHT_2_TM=60.277
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=2.0390
PRIMER_LEFT_3_PENALTY=1.715693
PRIMER_RIGHT_3_PENALTY=0.323355
PRIMER_LEFT_3_SEQUENCE=GTACGCGGGACATCCTCTCTAT
PRIMER_RIGHT_3_SEQUENCE=GAAGCTTTTGTTGCTCTTCACA
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=120,22
PRIMER_LEFT_3_TM=61.716
PRIMER_RIGHT_3_TM=59.677
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=2.0510
PRIMER_LEFT_4_PENALTY=1.982688
PRIMER_RIGHT_4_PENALTY=0.068302
PRIMER_LEFT_4_SEQUENCE=TACGCGGGACATCCTCTCTAT
PRIMER_RIGHT_4_SEQUENCE=TAAGGATCTTCCTCGGAAAACA
PRIMER_LEFT_4=8,21
PRIMER_RIGHT_4=247,22
PRIMER_LEFT_4_TM=60.983
PRIMER_RIGHT_4_TM=60.068
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_04360 Ps_contig_mira-and-tgicl-ass_franssen_33006
SEQUENCE=CGACAGAGTACGCGGGACATCCTCTCTATATATGCATTTTTTTTCTTTCCTCTTAATTTTATTATTATTATTATTGGAAAAAAATTGAGTAGTGTACTTTGTGAAGAGCAACAAAAGCTTCTTCTCAATCTCAAAAAATCTCTCACTATGAGAGACAACATTATCGACAACATCCCACCAAGAAAGCTCCTCACCCGCCGACAATCCACCAACACCTCCGCCGACATGTTTTCCGAGGAAGATCCTTATTCCTCCGACCACTTCCGCATGTACGAGTTCAAAATCCGGCGATGCACACGTAGCCGCAGCCACGACTGGACTGACTGTCCCTTCGCTCACCCTGGCGAAAAGGCTCGCCGCCGTGACCCTCTCCGGTTTCAATACTCCGGTGAAGTTTGCCCTGATTACCGCCGCGGAAACTGCGAACGCGGCGACGCGTGTGAATTCTCGCACGGCGTTTTTGAATGCTGGTTGCACCCTTCGAGATACAGAACTGAAGCTTGTAAAGATGGTAAGAATTGTAAGCGGAAAATTTGCTTTTTTGCTCATACACCCCGTCAACTCAGGGTTTTGCTTTTGCCACCCCCTCCTTCTCCACCTCCTCAGAACAACGTTAAAAACTGTTGTTCTTTTTGTCATTGCTGTTCTAATTCTTCTTCTTCGTCACCCACTTCTACTTTGTTGAGTGGACCTTATTTTTCTTCTTCGAATTCACCACCTTTGTCACCTTTGTCCAATTCAAATTCCAAAGATGTGCATGTTCTTAACGAGCTTATTCGATCTATGGAAAGTTTCAATTTTGGTGGTGATGATTCTCCTGTTTCTGCTTTGGGCGGTGGAAAATATTATAATTCAAGTGGTAGTAGCAAGTTTTTGAGGGAAGAACAAAGGATTAACGTTGTTGAGGATGATGTCAGTGCCAACCCAGATTTTGGGTGGGTGAATGAGTTGCTCATGTAGATGTTGATGATGATGGTGGTGATGTCAGTGCTGTTGTGGATATGATGATGGTGATGAGATGATGATGAATGGATGGTGATTTGATTAAGAAAATTAAGTGTAGATATAGTAATGTGATCATGTGAGTTATATGATCCTTAATTGGTTTGCTATGTCTCTATTTTTAATTGTAATTCAATTCGATCTTGTAATTTTTCAGTTTATTACTTTCTGAATTTGTACGTTTGTGAAAATTATATATTGTGTAATTTGTATTTCCATTAATAAAATAGATTTTATGCCTA
TARGET=651,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3306
PRIMER_LEFT_PENALTY=0.221115
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=GCACCCTTCGAGATACAGAACT
PRIMER_RIGHT_SEQUENCE=AGGTGGTGAATTCGAAGAAGAA
PRIMER_LEFT=473,22
PRIMER_RIGHT=719,22
PRIMER_LEFT_TM=59.779
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=247
PRIMER_PAIR_PENALTY_1=0.3306
PRIMER_LEFT_1_PENALTY=0.221115
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=GCACCCTTCGAGATACAGAACT
PRIMER_RIGHT_1_SEQUENCE=AAGGTGGTGAATTCGAAGAAGA
PRIMER_LEFT_1=473,22
PRIMER_RIGHT_1=720,22
PRIMER_LEFT_1_TM=59.779
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.3781
PRIMER_LEFT_2_PENALTY=0.325733
PRIMER_RIGHT_2_PENALTY=0.052366
PRIMER_LEFT_2_SEQUENCE=GTCAACTCAGGGTTTTGCTTTT
PRIMER_RIGHT_2_SEQUENCE=AAGCTCGTTAAGAACATGCACA
PRIMER_LEFT_2=556,22
PRIMER_RIGHT_2=773,22
PRIMER_LEFT_2_TM=59.674
PRIMER_RIGHT_2_TM=59.948
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=0.3859
PRIMER_LEFT_3_PENALTY=0.325733
PRIMER_RIGHT_3_PENALTY=0.060196
PRIMER_LEFT_3_SEQUENCE=GTCAACTCAGGGTTTTGCTTTT
PRIMER_RIGHT_3_SEQUENCE=GGAGAATCATCACCACCAAAAT
PRIMER_LEFT_3=556,22
PRIMER_RIGHT_3=817,22
PRIMER_LEFT_3_TM=59.674
PRIMER_RIGHT_3_TM=60.060
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.4352
PRIMER_LEFT_4_PENALTY=0.325733
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=GTCAACTCAGGGTTTTGCTTTT
PRIMER_RIGHT_4_SEQUENCE=AAGGTGGTGAATTCGAAGAAGA
PRIMER_LEFT_4=556,22
PRIMER_RIGHT_4=720,22
PRIMER_LEFT_4_TM=59.674
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_04361 Ps_contig_mira-and-tgicl-ass_franssen_33011
SEQUENCE=GTTAATTGTGAAAATTAGACATTGCCATAAATGTAGAACATTATTGAAGAAAGAACATTTATGTTGAAGCAACAATCAAATTCACATACAAAAGAAATCAGCTACACCCAACAACAGCAACAACCTTAAATCTAACCCACATATACTAAAGTAACATTTTAAAGGTGTTAAACAAAAGAATTTTCACCATCACCATCATCATCATCATTATCATTATCATTATCATCATCATCATCACAATCATAATCACTGTACAAGCACAAGTCTACCACCCATACCTCTATCACTATCACCACCTTCAACATTCTGCAGATCATGCCCCTTCGGCACAGTAACAATCAACTCCCCATCAACAAACACCGCACTCGCAAGCTCTGGCAGAGTCGAATCCGGCAAACGAAACCTCCACATATCAAGCTCTAACTGATCAAGCGACAACTCCAAAGATTCACCATCTCTAACAACAATCTTCGTCACGCCGGGATGGATCTCCACCGTGTGAGTCCTCACCTCACCGATACCCTCCGTCTCAACCACAAACCGGAAACAATCAGGCGCCTCTTCAATCGCCACGTCAGCATCCGATCGAAACGGAAGTTCCAATACGCGGCTGAAAACGTGTGGGAGACGCCGTAATTTCTTCCCGGATATTCCCAACAAAGCCTGAGCCTCCGACGCAGGGTTGCTTCCGTTAGTTCCGAATTGGATTGTGATGTTGCGCTTTCTCGGAGCAGGGTGAATCTTCATGGTGTTACCGTTTAACGCCGCCGCAGAGGATTGAACCGCCACCACCAACAACAACAACAAAAAGATTAACACACCGATCAAATTTACCTTTCCGATTGAAGAAAGAATCATAAAGGATTGATTCTTGTTCTCGTTTTTCCGACGCGTTTCTTATTTGGGGAAGAAAATGAAAAACCTAACCCTAATTTGTCGATTCGATTTGAGAATTGAAATGAAACGTGATTTGAGATTATTTATTTTTTTCTCATTTTTTAAGGATATTGAAAATTGAAAAAGTTTGAGAAACTGTTATTTGGTGAAATGGGTAATGCAAAGTGCCCC
TARGET=194,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7838
PRIMER_LEFT_PENALTY=0.460467
PRIMER_RIGHT_PENALTY=0.323355
PRIMER_LEFT_SEQUENCE=AAATCAGCTACACCCAACAACA
PRIMER_RIGHT_SEQUENCE=GTTGATTGTTACTGTGCCGAAG
PRIMER_LEFT=94,22
PRIMER_RIGHT=342,22
PRIMER_LEFT_TM=59.540
PRIMER_RIGHT_TM=59.677
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.9088
PRIMER_LEFT_1_PENALTY=0.733091
PRIMER_RIGHT_1_PENALTY=0.175676
PRIMER_LEFT_1_SEQUENCE=CAAAAGAAATCAGCTACACCCA
PRIMER_RIGHT_1_SEQUENCE=GAGTTGATTGTTACTGTGCCGA
PRIMER_LEFT_1=88,22
PRIMER_RIGHT_1=344,22
PRIMER_LEFT_1_TM=59.267
PRIMER_RIGHT_1_TM=60.176
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.9393
PRIMER_LEFT_2_PENALTY=0.830594
PRIMER_RIGHT_2_PENALTY=0.108684
PRIMER_LEFT_2_SEQUENCE=AATCAGCTACACCCAACAACAG
PRIMER_RIGHT_2_SEQUENCE=TGGGGAGTTGATTGTTACTGTG
PRIMER_LEFT_2=95,22
PRIMER_RIGHT_2=348,22
PRIMER_LEFT_2_TM=59.169
PRIMER_RIGHT_2_TM=59.891
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=0.9928
PRIMER_LEFT_3_PENALTY=0.733091
PRIMER_RIGHT_3_PENALTY=0.259704
PRIMER_LEFT_3_SEQUENCE=CAAAAGAAATCAGCTACACCCA
PRIMER_RIGHT_3_SEQUENCE=GATCTGCAGAATGTTGAAGGTG
PRIMER_LEFT_3=88,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=59.267
PRIMER_RIGHT_3_TM=59.740
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=1.0564
PRIMER_LEFT_4_PENALTY=0.733091
PRIMER_RIGHT_4_PENALTY=0.323355
PRIMER_LEFT_4_SEQUENCE=CAAAAGAAATCAGCTACACCCA
PRIMER_RIGHT_4_SEQUENCE=GTTGATTGTTACTGTGCCGAAG
PRIMER_LEFT_4=88,22
PRIMER_RIGHT_4=342,22
PRIMER_LEFT_4_TM=59.267
PRIMER_RIGHT_4_TM=59.677
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_04362 Ps_contig_mira-and-tgicl-ass_franssen_33011
SEQUENCE=GTTAATTGTGAAAATTAGACATTGCCATAAATGTAGAACATTATTGAAGAAAGAACATTTATGTTGAAGCAACAATCAAATTCACATACAAAAGAAATCAGCTACACCCAACAACAGCAACAACCTTAAATCTAACCCACATATACTAAAGTAACATTTTAAAGGTGTTAAACAAAAGAATTTTCACCATCACCATCATCATCATCATTATCATTATCATTATCATCATCATCATCACAATCATAATCACTGTACAAGCACAAGTCTACCACCCATACCTCTATCACTATCACCACCTTCAACATTCTGCAGATCATGCCCCTTCGGCACAGTAACAATCAACTCCCCATCAACAAACACCGCACTCGCAAGCTCTGGCAGAGTCGAATCCGGCAAACGAAACCTCCACATATCAAGCTCTAACTGATCAAGCGACAACTCCAAAGATTCACCATCTCTAACAACAATCTTCGTCACGCCGGGATGGATCTCCACCGTGTGAGTCCTCACCTCACCGATACCCTCCGTCTCAACCACAAACCGGAAACAATCAGGCGCCTCTTCAATCGCCACGTCAGCATCCGATCGAAACGGAAGTTCCAATACGCGGCTGAAAACGTGTGGGAGACGCCGTAATTTCTTCCCGGATATTCCCAACAAAGCCTGAGCCTCCGACGCAGGGTTGCTTCCGTTAGTTCCGAATTGGATTGTGATGTTGCGCTTTCTCGGAGCAGGGTGAATCTTCATGGTGTTACCGTTTAACGCCGCCGCAGAGGATTGAACCGCCACCACCAACAACAACAACAAAAAGATTAACACACCGATCAAATTTACCTTTCCGATTGAAGAAAGAATCATAAAGGATTGATTCTTGTTCTCGTTTTTCCGACGCGTTTCTTATTTGGGGAAGAAAATGAAAAACCTAACCCTAATTTGTCGATTCGATTTGAGAATTGAAATGAAACGTGATTTGAGATTATTTATTTTTTTCTCATTTTTTAAGGATATTGAAAATTGAAAAAGTTTGAGAAACTGTTATTTGGTGAAATGGGTAATGCAAAGTGCCCC
TARGET=222,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7288
PRIMER_LEFT_PENALTY=0.591301
PRIMER_RIGHT_PENALTY=0.137489
PRIMER_LEFT_SEQUENCE=TTTCACCATCACCATCATCATC
PRIMER_RIGHT_SEQUENCE=ATCTTTGGAGTTGTCGCTTGAT
PRIMER_LEFT=181,22
PRIMER_RIGHT=447,22
PRIMER_LEFT_TM=60.591
PRIMER_RIGHT_TM=60.137
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.7304
PRIMER_LEFT_1_PENALTY=0.591301
PRIMER_RIGHT_1_PENALTY=0.139129
PRIMER_LEFT_1_SEQUENCE=TTTCACCATCACCATCATCATC
PRIMER_RIGHT_1_SEQUENCE=TGATATGTGGAGGTTTCGTTTG
PRIMER_LEFT_1=181,22
PRIMER_RIGHT_1=414,22
PRIMER_LEFT_1_TM=60.591
PRIMER_RIGHT_1_TM=59.861
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.7670
PRIMER_LEFT_2_PENALTY=0.591301
PRIMER_RIGHT_2_PENALTY=0.175676
PRIMER_LEFT_2_SEQUENCE=TTTCACCATCACCATCATCATC
PRIMER_RIGHT_2_SEQUENCE=GAGTTGATTGTTACTGTGCCGA
PRIMER_LEFT_2=181,22
PRIMER_RIGHT_2=344,22
PRIMER_LEFT_2_TM=60.591
PRIMER_RIGHT_2_TM=60.176
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.7838
PRIMER_LEFT_3_PENALTY=0.460467
PRIMER_RIGHT_3_PENALTY=0.323355
PRIMER_LEFT_3_SEQUENCE=AAATCAGCTACACCCAACAACA
PRIMER_RIGHT_3_SEQUENCE=GTTGATTGTTACTGTGCCGAAG
PRIMER_LEFT_3=94,22
PRIMER_RIGHT_3=342,22
PRIMER_LEFT_3_TM=59.540
PRIMER_RIGHT_3_TM=59.677
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.8006
PRIMER_LEFT_4_PENALTY=0.591301
PRIMER_RIGHT_4_PENALTY=0.209271
PRIMER_LEFT_4_SEQUENCE=TTTCACCATCACCATCATCATC
PRIMER_RIGHT_4_SEQUENCE=TCGCTTGATCAGTTAGAGCTTG
PRIMER_LEFT_4=181,22
PRIMER_RIGHT_4=434,22
PRIMER_LEFT_4_TM=60.591
PRIMER_RIGHT_4_TM=59.791
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_04363 Ps_contig_mira-and-tgicl-ass_franssen_33011
SEQUENCE=GTTAATTGTGAAAATTAGACATTGCCATAAATGTAGAACATTATTGAAGAAAGAACATTTATGTTGAAGCAACAATCAAATTCACATACAAAAGAAATCAGCTACACCCAACAACAGCAACAACCTTAAATCTAACCCACATATACTAAAGTAACATTTTAAAGGTGTTAAACAAAAGAATTTTCACCATCACCATCATCATCATCATTATCATTATCATTATCATCATCATCATCACAATCATAATCACTGTACAAGCACAAGTCTACCACCCATACCTCTATCACTATCACCACCTTCAACATTCTGCAGATCATGCCCCTTCGGCACAGTAACAATCAACTCCCCATCAACAAACACCGCACTCGCAAGCTCTGGCAGAGTCGAATCCGGCAAACGAAACCTCCACATATCAAGCTCTAACTGATCAAGCGACAACTCCAAAGATTCACCATCTCTAACAACAATCTTCGTCACGCCGGGATGGATCTCCACCGTGTGAGTCCTCACCTCACCGATACCCTCCGTCTCAACCACAAACCGGAAACAATCAGGCGCCTCTTCAATCGCCACGTCAGCATCCGATCGAAACGGAAGTTCCAATACGCGGCTGAAAACGTGTGGGAGACGCCGTAATTTCTTCCCGGATATTCCCAACAAAGCCTGAGCCTCCGACGCAGGGTTGCTTCCGTTAGTTCCGAATTGGATTGTGATGTTGCGCTTTCTCGGAGCAGGGTGAATCTTCATGGTGTTACCGTTTAACGCCGCCGCAGAGGATTGAACCGCCACCACCAACAACAACAACAAAAAGATTAACACACCGATCAAATTTACCTTTCCGATTGAAGAAAGAATCATAAAGGATTGATTCTTGTTCTCGTTTTTCCGACGCGTTTCTTATTTGGGGAAGAAAATGAAAAACCTAACCCTAATTTGTCGATTCGATTTGAGAATTGAAATGAAACGTGATTTGAGATTATTTATTTTTTTCTCATTTTTTAAGGATATTGAAAATTGAAAAAGTTTGAGAAACTGTTATTTGGTGAAATGGGTAATGCAAAGTGCCCC
TARGET=793,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1430
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=GATATTCCCAACAAAGCCTGAG
PRIMER_RIGHT_SEQUENCE=CGGAAAAACGAGAACAAGAATC
PRIMER_LEFT=646,22
PRIMER_RIGHT=887,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.3179
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.283783
PRIMER_LEFT_1_SEQUENCE=GATATTCCCAACAAAGCCTGAG
PRIMER_RIGHT_1_SEQUENCE=GGAAAGGTAAATTTGATCGGTG
PRIMER_LEFT_1=646,22
PRIMER_RIGHT_1=839,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.716
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=194
PRIMER_PAIR_PENALTY_2=0.4390
PRIMER_LEFT_2_PENALTY=0.330118
PRIMER_RIGHT_2_PENALTY=0.108929
PRIMER_LEFT_2_SEQUENCE=TAATTTCTTCCCGGATATTCCC
PRIMER_RIGHT_2_SEQUENCE=CGGAAAAACGAGAACAAGAATC
PRIMER_LEFT_2=633,22
PRIMER_RIGHT_2=887,22
PRIMER_LEFT_2_TM=60.330
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=0.4870
PRIMER_LEFT_3_PENALTY=0.378093
PRIMER_RIGHT_3_PENALTY=0.108929
PRIMER_LEFT_3_SEQUENCE=CCGTAATTTCTTCCCGGATATT
PRIMER_RIGHT_3_SEQUENCE=CGGAAAAACGAGAACAAGAATC
PRIMER_LEFT_3=630,22
PRIMER_RIGHT_3=887,22
PRIMER_LEFT_3_TM=60.378
PRIMER_RIGHT_3_TM=60.109
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=0.5205
PRIMER_LEFT_4_PENALTY=0.236697
PRIMER_RIGHT_4_PENALTY=0.283783
PRIMER_LEFT_4_SEQUENCE=CGTTAGTTCCGAATTGGATTGT
PRIMER_RIGHT_4_SEQUENCE=GGAAAGGTAAATTTGATCGGTG
PRIMER_LEFT_4=689,22
PRIMER_RIGHT_4=839,22
PRIMER_LEFT_4_TM=60.237
PRIMER_RIGHT_4_TM=59.716
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_04364 Ps_contig_mira-and-tgicl-ass_franssen_33012
SEQUENCE=TAACAAGCTANACCCCAACCAAACCAAGCAACAACCCTTAAAATATAACCCACCATATACTAAAGTAACATTTAAAGGTTGTTTAACTAAAAGAAATTTTACTACCATCACCATCATCATCATCATTATCATTATCATTATCATCATCATCATCACAATCATAATCACTGTACAAGCACAAGTCTACCACCCATACCTCTATCACTATCACCACCTTCAACATTCTGCAGATCATGCCCCTTCGGCACAGTAACAATCAACTCCCCATCAACAAACCCCG
TARGET=112,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5800
PRIMER_LEFT_PENALTY=0.404310
PRIMER_RIGHT_PENALTY=0.175676
PRIMER_LEFT_SEQUENCE=TAACAAGCTANACCCCAACCAA
PRIMER_RIGHT_SEQUENCE=GAGTTGATTGTTACTGTGCCGA
PRIMER_LEFT=0,22
PRIMER_RIGHT=262,22
PRIMER_LEFT_TM=60.404
PRIMER_RIGHT_TM=60.176
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.6640
PRIMER_LEFT_1_PENALTY=0.404310
PRIMER_RIGHT_1_PENALTY=0.259704
PRIMER_LEFT_1_SEQUENCE=TAACAAGCTANACCCCAACCAA
PRIMER_RIGHT_1_SEQUENCE=GATCTGCAGAATGTTGAAGGTG
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=60.404
PRIMER_RIGHT_1_TM=59.740
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.7277
PRIMER_LEFT_2_PENALTY=0.404310
PRIMER_RIGHT_2_PENALTY=0.323355
PRIMER_LEFT_2_SEQUENCE=TAACAAGCTANACCCCAACCAA
PRIMER_RIGHT_2_SEQUENCE=GTTGATTGTTACTGTGCCGAAG
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=260,22
PRIMER_LEFT_2_TM=60.404
PRIMER_RIGHT_2_TM=59.677
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.7277
PRIMER_LEFT_3_PENALTY=0.404310
PRIMER_RIGHT_3_PENALTY=0.323355
PRIMER_LEFT_3_SEQUENCE=TAACAAGCTANACCCCAACCAA
PRIMER_RIGHT_3_SEQUENCE=AGTTGATTGTTACTGTGCCGAA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=60.404
PRIMER_RIGHT_3_TM=59.677
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=1.1866
PRIMER_LEFT_4_PENALTY=0.404310
PRIMER_RIGHT_4_PENALTY=0.782312
PRIMER_LEFT_4_SEQUENCE=TAACAAGCTANACCCCAACCAA
PRIMER_RIGHT_4_SEQUENCE=GGGAGTTGATTGTTACTGTGCC
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=264,22
PRIMER_LEFT_4_TM=60.404
PRIMER_RIGHT_4_TM=60.782
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=7.75
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_04365 Ps_contig_mira-and-tgicl-ass_franssen_33012
SEQUENCE=TAACAAGCTANACCCCAACCAAACCAAGCAACAACCCTTAAAATATAACCCACCATATACTAAAGTAACATTTAAAGGTTGTTTAACTAAAAGAAATTTTACTACCATCACCATCATCATCATCATTATCATTATCATTATCATCATCATCATCACAATCATAATCACTGTACAAGCACAAGTCTACCACCCATACCTCTATCACTATCACCACCTTCAACATTCTGCAGATCATGCCCCTTCGGCACAGTAACAATCAACTCCCCATCAACAAACCCCG
TARGET=140,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5800
PRIMER_LEFT_PENALTY=0.404310
PRIMER_RIGHT_PENALTY=0.175676
PRIMER_LEFT_SEQUENCE=TAACAAGCTANACCCCAACCAA
PRIMER_RIGHT_SEQUENCE=GAGTTGATTGTTACTGTGCCGA
PRIMER_LEFT=0,22
PRIMER_RIGHT=262,22
PRIMER_LEFT_TM=60.404
PRIMER_RIGHT_TM=60.176
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.6640
PRIMER_LEFT_1_PENALTY=0.404310
PRIMER_RIGHT_1_PENALTY=0.259704
PRIMER_LEFT_1_SEQUENCE=TAACAAGCTANACCCCAACCAA
PRIMER_RIGHT_1_SEQUENCE=GATCTGCAGAATGTTGAAGGTG
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=60.404
PRIMER_RIGHT_1_TM=59.740
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.7277
PRIMER_LEFT_2_PENALTY=0.404310
PRIMER_RIGHT_2_PENALTY=0.323355
PRIMER_LEFT_2_SEQUENCE=TAACAAGCTANACCCCAACCAA
PRIMER_RIGHT_2_SEQUENCE=GTTGATTGTTACTGTGCCGAAG
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=260,22
PRIMER_LEFT_2_TM=60.404
PRIMER_RIGHT_2_TM=59.677
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.7277
PRIMER_LEFT_3_PENALTY=0.404310
PRIMER_RIGHT_3_PENALTY=0.323355
PRIMER_LEFT_3_SEQUENCE=TAACAAGCTANACCCCAACCAA
PRIMER_RIGHT_3_SEQUENCE=AGTTGATTGTTACTGTGCCGAA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=60.404
PRIMER_RIGHT_3_TM=59.677
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=1.1866
PRIMER_LEFT_4_PENALTY=0.404310
PRIMER_RIGHT_4_PENALTY=0.782312
PRIMER_LEFT_4_SEQUENCE=TAACAAGCTANACCCCAACCAA
PRIMER_RIGHT_4_SEQUENCE=GGGAGTTGATTGTTACTGTGCC
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=264,22
PRIMER_LEFT_4_TM=60.404
PRIMER_RIGHT_4_TM=60.782
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=7.75
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_04366 Ps_contig_mira-and-tgicl-ass_franssen_33014
SEQUENCE=CCAAACCCTTTTCACCACCTGGAAACCCTCCGGTTAGCCCCTATTTTTTCTTTTATTTTTTTCCTTTTTCTCAAAACCATCTTCTGGAATTTCAACAGTCAACTTTTCTGAGTTTTCTTGCACTTTTTCATTGGATAACGATGATGATGATGATGATGATGATGATGATTCCTCTACATCAACTTTCCTTTTCAGCTCTTTTTCAACTCCTATTTCTCCATTGCATAGTCCTCTTCCTCCCATCATTTTTAGTATGTTGAATTCAGCACATGTTTGTGGTTGACAAGTTAGATTTCTATGATTTCTTTTGGATGTTTTTATGAGAGGAAAGTGAAGATCTTGGCCTAGGAATTGGGATCTTAGAAGAGAACCATGTAGGCCTTGAACAGTAGCAAAAGTAACCATTTTGAAGTGAAGTTAGGTGGAAAAAAGAAAAGAGTTATGATGTAATCTTCTTAAACATGTTAATGAGTTTTGGATGTATAGCTCAAAAATCTAGATGAATAGATTCCTCCCCCG
TARGET=140,30
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7397
PRIMER_LEFT_PENALTY=1.644169
PRIMER_RIGHT_PENALTY=0.095574
PRIMER_LEFT_SEQUENCE=CTCCGGTTAGCCCCTATTTTT
PRIMER_RIGHT_SEQUENCE=GGAAGAGGACTATGCAATGGAG
PRIMER_LEFT=27,21
PRIMER_RIGHT=236,22
PRIMER_LEFT_TM=60.644
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=1.7397
PRIMER_LEFT_1_PENALTY=1.644169
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=CTCCGGTTAGCCCCTATTTTT
PRIMER_RIGHT_1_SEQUENCE=GAGGAAGAGGACTATGCAATGG
PRIMER_LEFT_1=27,21
PRIMER_RIGHT_1=238,22
PRIMER_LEFT_1_TM=60.644
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=1.7397
PRIMER_LEFT_2_PENALTY=1.644169
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=CTCCGGTTAGCCCCTATTTTT
PRIMER_RIGHT_2_SEQUENCE=GGAGGAAGAGGACTATGCAATG
PRIMER_LEFT_2=27,21
PRIMER_RIGHT_2=239,22
PRIMER_LEFT_2_TM=60.644
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=213
PRIMER_PAIR_PENALTY_3=1.7397
PRIMER_LEFT_3_PENALTY=1.644169
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=CTCCGGTTAGCCCCTATTTTT
PRIMER_RIGHT_3_SEQUENCE=AGGAAGAGGACTATGCAATGGA
PRIMER_LEFT_3=27,21
PRIMER_RIGHT_3=237,22
PRIMER_LEFT_3_TM=60.644
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=1.9607
PRIMER_LEFT_4_PENALTY=1.644169
PRIMER_RIGHT_4_PENALTY=0.316570
PRIMER_LEFT_4_SEQUENCE=CTCCGGTTAGCCCCTATTTTT
PRIMER_RIGHT_4_SEQUENCE=AAATGATGGGAGGAAGAGGACT
PRIMER_LEFT_4=27,21
PRIMER_RIGHT_4=247,22
PRIMER_LEFT_4_TM=60.644
PRIMER_RIGHT_4_TM=60.317
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_04367 Ps_contig_mira-and-tgicl-ass_franssen_33016
SEQUENCE=TCGTCGGGGAAGAATACATATATACACATTAACGCATAGTTAAACAAAGTNATAACCAAACACTCACATTCAAGTCAAAGATAAAAAGAAAGATCGAGAAAGTTGCAACAAAAGCCATGGAAGGTTTACAAAGATCCGCAGTTTCCTTCCGGCGACAAGGTTCATCAGGATTTGTTTGGGATGATCGGTTTCTACAAGAAGAAATCAACAAAATGAACCAGAGTCAGCAGAATAATAATCAAGAACAAGAGAAAGATGAAATCAAAGAGATGATGAATCGTGATGAGCCTCCGCAGCTTGGGTCGATCAATACTGTGCAGAGAAGTCGTTCTAACGGTGGTGGAGGTCGTGGTTATCGTACTGGAAAAGTTTCGCCGGCGATTGAGCCGCCGTCTCCGAGGATCTCCGTGTGTGGATTTTGTGGTGCTTTTGGAAAAGTTGGAGAGAAGACGAAACCGCCTAAGCATCGATCTAGGTGATTGGGTGGTGGTTTGTGTTGGTCGGTGGTTATAGTTGCCGGAGATGGGATATTCCGGTGAAGGTGAAAGGTTATGATGTTTTTTTTTGTAATATTAATTACTTTAGGTTTGTGGGGTAGTAGTGCCTAGTGGTTGAGTTGAGTTGAGTTCATCATGAATTTGTATTGTTAATTAAGAACATAATGATGATGGGTCATGTTTTATTTTCCTTTTGCTTACTTTTATTATTACATANGTATTTAGAGTATTAAACTTTTTGTT
TARGET=611,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04368 Ps_contig_mira-and-tgicl-ass_franssen_33019
SEQUENCE=ATCTTTGGCGCTTACAACATGTGACTTCTGGTTCTTGTCTCTCTCCTCTTTTCCCTCACACAACAAAACAAAAACATCATACTTTTCTTTTCTCACTCTTCTTCTTCTTCTCATCATGGCTTTCTCTACTCCTCTCAAACTCATTCTAACCATTCTACTATCTCTCACTCTTTTCACCATCATCAACATTCACGTTGATGCACAGATCGAAGAAACAACCTTGAACCTTAGTTCTGATGTTCTAGAATGGCCGACAACAATGTCATCACTCTACAATGAACTAGCTGAAGACAATGAAGAAGACATAGACAGTGATTTCAGTCGAAGATCTTTGTTCTGGAGTAGAGTGAAATACTATATCTCTTACGGTGCTCTAGCTGCTAACAGAATCCCTTGTCCTCCTCGTTCTGGTAGATCTTATTACACTCACAAGTGTTATGAAGCTAGAGGACCTGTTCATCCTTATACTAGAGGTTGTTCTGCTATCACGCGATGCAGAAGATGAAATCATGAGGTTTTGTTTTTTATTTCTATTCTCAGATCGAGAATTAATTGAAATTTCAAGTTAGGTAAAGTTTGTGTTAGCTTTATTGTTAAGTTAATTAAAGCATTATTAGCAGCATATTGTGTGCTATGTGTTTGAACACTGAGTGGATTTCAAACTGTCATTTATTTTTTTGTGTATGATGGGTTCTTCTTTTATGGATTTAATTTGTAGTACTATATGCACTGCAGAAATGGATGTGTTATGTGTATGTATTTTGTGTTGCGTCATGGCCTATTTCTTTATATYGTTTGTATAAATATTGCTTCCACAATGATTCAATTCAATCGATCATTT
TARGET=97,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04369 Ps_contig_mira-and-tgicl-ass_franssen_33022
SEQUENCE=TTCGGGGACTGATTTGTTTTACAAAAATAAGGCTAAAGTCCCATATATCACGACTGCTCATCAGTCCCTAACCTCAAACAACACTCAAAACCCAAAACCCTAGAAACCGGCGATTCCATGGATTTGCAATCGAACGATTTCGATCGCCTTTTGTTCTTCGAGCACGCTCGTAAAACTGCTGAAGCTGAATACCTTGCCAACCCTCTCGATGTTGACAATTTGACAAGATGGGGTGGAGCTTTGTTAGAGTTGTCTCAGTTTCAGAATTTTCCCGAGTCAAAGAAGATGACCCAAGAGGCTATTTCAAAGCTTGAGGAGGCCATTTCTCTGAATCCCAACAAGCACGATGCTCTTTGGTGCCTGGGAAATGCTCTCACTTCGCAAGCATTTTTAAACCCAGACCCGGATGAAGCTAAAGTTCATTTTGACAGGGCAGCTGTGTACTTTCAACAAGCTGTTGATGAGGACCCTTCAAATGAACTTTACCGCAAATCATTGGAAGTCGCTGCTAAGGCTCCCGAGCTACATGTAGAAATCCATAAGCATGGATTAGGTCAGCAATCAGCAGGGTCGGCTGGGCCTTCTTCTTCTTCTGGTGCAAAGACTCAAAAGAAAAAGAAAGACAGTGATCTGAAATATGACATATTTGGATGGATAATCTTGGCTGTTGGCATTGTTACATGGGTGGGGTTAGCAAAATCGAACCTTCCACCACCGCCTCCTCCTCCACGATAAGTGAACCCATTTCATTTAATTTGATACATTGTTGGTTATTGAGAATCACATCTGATTTCGGCAGCAACTTGTTGTTTCGTTTTTGACTGTTGTAATATTTTTCATGGTGCAACCATTTATCTACCCATCATATGAAATTTTATCATATTATTATAATTGGGTTGAGTAGTTATCAATGGTGGTCTATAACACCCAGAAAACTGGCTGACATATTTGGTTTGAAGATATTTTTATACACTAATAAAAACATAAATTTTTGTTCTTT
TARGET=581,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1355
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.128591
PRIMER_LEFT_SEQUENCE=AAGCATGGATTAGGTCAGCAAT
PRIMER_RIGHT_SEQUENCE=CTAACCCCACCCATGTAACAAT
PRIMER_LEFT=540,22
PRIMER_RIGHT=693,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.871
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.1461
PRIMER_LEFT_1_PENALTY=0.006951
PRIMER_RIGHT_1_PENALTY=0.139129
PRIMER_LEFT_1_SEQUENCE=AAGCATGGATTAGGTCAGCAAT
PRIMER_RIGHT_1_SEQUENCE=TGAAATGGGTTCACTTATCGTG
PRIMER_LEFT_1=540,22
PRIMER_RIGHT_1=748,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=59.861
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.1521
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.128591
PRIMER_LEFT_2_SEQUENCE=TGAGGACCCTTCAAATGAACTT
PRIMER_RIGHT_2_SEQUENCE=CTAACCCCACCCATGTAACAAT
PRIMER_LEFT_2=461,22
PRIMER_RIGHT_2=693,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=59.871
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.1627
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.139129
PRIMER_LEFT_3_SEQUENCE=TGAGGACCCTTCAAATGAACTT
PRIMER_RIGHT_3_SEQUENCE=TGAAATGGGTTCACTTATCGTG
PRIMER_LEFT_3=461,22
PRIMER_RIGHT_3=748,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=59.861
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.2485
PRIMER_LEFT_4_PENALTY=0.006951
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=AAGCATGGATTAGGTCAGCAAT
PRIMER_RIGHT_4_SEQUENCE=AATGGGTTCACTTATCGTGGAG
PRIMER_LEFT_4=540,22
PRIMER_RIGHT_4=745,22
PRIMER_LEFT_4_TM=59.993
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=206
=
PRIMER_SEQUENCE_ID=SSR_04370 Ps_contig_mira-and-tgicl-ass_franssen_33025
SEQUENCE=ACCTTCTCTACATTGAAACCAATTGTAGGAACGGTGGATAAAACTTCTCCAATGTGAAGTTTGTAGAGTATAGTTGTTTTTCCAGCGGCATCAAGACCAAGCATGACAACCGCATCTCGGTATTGCCGAAGAAGGTATCGAAAAGCTTGCGAAAAGCTTGACCCATTCGATCAAAATCAAAGAAAATCGTTGCTTGATTGAATATACGATTCTTGGAAACCGAAAAACAGGTTTTTGGATTTCAAAATGCAAAGGGAAGGGAAGGAAAGGGGGATTGAATTGAATTGAATTGCGTTTCCTTCAACCTTCCCTGTTGCGTTTGATTTGGAAAAATGAATTCTAAGCACCTTGTTATTATGATACTAGCTTAAACAGAATGGGAATCTTTCACCTTTT
TARGET=274,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7528
PRIMER_LEFT_PENALTY=0.029729
PRIMER_RIGHT_PENALTY=0.723090
PRIMER_LEFT_SEQUENCE=GTATAGTTGTTTTTCCAGCGGC
PRIMER_RIGHT_SEQUENCE=AACAGGGAAGGTTGAAGGAAAC
PRIMER_LEFT=67,22
PRIMER_RIGHT=314,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=60.723
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=12.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.9139
PRIMER_LEFT_1_PENALTY=0.373881
PRIMER_RIGHT_1_PENALTY=0.540037
PRIMER_LEFT_1_SEQUENCE=GTATCGAAAAGCTTGCGAAAAG
PRIMER_RIGHT_1_SEQUENCE=AAGGTGAAAGATTCCCATTCTG
PRIMER_LEFT_1=134,22
PRIMER_RIGHT_1=393,22
PRIMER_LEFT_1_TM=60.374
PRIMER_RIGHT_1_TM=59.460
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=260
PRIMER_PAIR_PENALTY_2=1.0440
PRIMER_LEFT_2_PENALTY=0.373881
PRIMER_RIGHT_2_PENALTY=0.670093
PRIMER_LEFT_2_SEQUENCE=GTATCGAAAAGCTTGCGAAAAG
PRIMER_RIGHT_2_SEQUENCE=GGTGAAAGATTCCCATTCTGTT
PRIMER_LEFT_2=134,22
PRIMER_RIGHT_2=391,22
PRIMER_LEFT_2_TM=60.374
PRIMER_RIGHT_2_TM=59.330
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=1.0672
PRIMER_LEFT_3_PENALTY=0.029729
PRIMER_RIGHT_3_PENALTY=1.037486
PRIMER_LEFT_3_SEQUENCE=GTATAGTTGTTTTTCCAGCGGC
PRIMER_RIGHT_3_SEQUENCE=AACAGGGAAGGTTGAAGGAAA
PRIMER_LEFT_3=67,22
PRIMER_RIGHT_3=314,21
PRIMER_LEFT_3_TM=60.030
PRIMER_RIGHT_3_TM=59.963
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=12.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=248
PRIMER_PAIR_PENALTY_4=1.0970
PRIMER_LEFT_4_PENALTY=0.373881
PRIMER_RIGHT_4_PENALTY=0.723090
PRIMER_LEFT_4_SEQUENCE=GTATCGAAAAGCTTGCGAAAAG
PRIMER_RIGHT_4_SEQUENCE=AACAGGGAAGGTTGAAGGAAAC
PRIMER_LEFT_4=134,22
PRIMER_RIGHT_4=314,22
PRIMER_LEFT_4_TM=60.374
PRIMER_RIGHT_4_TM=60.723
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_04371 Ps_contig_mira-and-tgicl-ass_franssen_33030
SEQUENCE=GGTTAAGAAAAAGGTTCTCTTTCAGACTTCATCTTATAGCACAATACCCTGTTGTTCAACATATTCTACAGTTCGTATATATGATAAAGAAAATTCAATATTACAACAGCAAAATCTTCTAACAAAGCATACATTTGTACCTACCAATTCATCACCGCCTTCTTCGGTTGCTGTAATCTTCGTCTTCACCTTCTTTATCCTTTAAGAAGCCAAGAAAAGCAAGAAACCCAAAAAAGAAAAATCCACCAGACCAATTGCCTCCACCTCCACCTCCTCCCCCGCCATCATCATCATCACGCGGCGGAAATCCAGATCCACCATCATCGATTTGTGGGGACTTCTCCTTACCGGGAAGATGAGTGATTGTCGGAGCCTCTCGAGTAACAGTCTGAGTTTGCTCTGCACCACATCTTGCATCCAAAGCTGCAGCACATATTATAGTTGCTGCCTGAGTAGAACGGGCTCTTCTATCAAATGGAAACTGTGTGCGACTCAATGATATTGAGTTATTAACATTCAGTAATCCTCTTGAAAATATAGATGTAGGAACAACGTTCTCTTCAATGCAGTTTCCACCTCTTCTCACTGGTGTTAATTTGGGCAAAGATGCAACTGCACTAGTGGTCAACATTGGTGTATGACAGATAACACACGGAGTTGTCGATGTGTTCGGATAAGGTGAGTTGTATGAACTGAAAAGTATGAAAAGGTTGCGAAATTGGGTTCAATTGTGGATTAATTTGAGGCTCCGAGAGAGAGAAAGTTGAAACCCTAACAATGGTTTGGAAGAAGAAGAAGAAAAAGAGGAAGACGATAGATAGACGAAGCAGGTTGGTCGCAAAATCTCAAGGACCTTGCAAATTGCCACCACCTCCCATATTT
TARGET=283,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2491
PRIMER_LEFT_PENALTY=0.216588
PRIMER_RIGHT_PENALTY=0.032476
PRIMER_LEFT_SEQUENCE=CCTTCTTCGGTTGCTGTAATCT
PRIMER_RIGHT_SEQUENCE=ATCTTCCCGGTAAGGAGAAGTC
PRIMER_LEFT=157,22
PRIMER_RIGHT=356,22
PRIMER_LEFT_TM=59.783
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=200
PRIMER_PAIR_PENALTY_1=0.2860
PRIMER_LEFT_1_PENALTY=0.253482
PRIMER_RIGHT_1_PENALTY=0.032476
PRIMER_LEFT_1_SEQUENCE=TGTACCTACCAATTCATCACCG
PRIMER_RIGHT_1_SEQUENCE=ATCTTCCCGGTAAGGAGAAGTC
PRIMER_LEFT_1=135,22
PRIMER_RIGHT_1=356,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.4539
PRIMER_LEFT_2_PENALTY=0.216588
PRIMER_RIGHT_2_PENALTY=0.237277
PRIMER_LEFT_2_SEQUENCE=CCTTCTTCGGTTGCTGTAATCT
PRIMER_RIGHT_2_SEQUENCE=TGCAGAGCAAACTCAGACTGTT
PRIMER_LEFT_2=157,22
PRIMER_RIGHT_2=403,22
PRIMER_LEFT_2_TM=59.783
PRIMER_RIGHT_2_TM=60.237
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.4883
PRIMER_LEFT_3_PENALTY=0.455824
PRIMER_RIGHT_3_PENALTY=0.032476
PRIMER_LEFT_3_SEQUENCE=GAAGCCAAGAAAAGCAAGAAAC
PRIMER_RIGHT_3_SEQUENCE=ATCTTCCCGGTAAGGAGAAGTC
PRIMER_LEFT_3=205,22
PRIMER_RIGHT_3=356,22
PRIMER_LEFT_3_TM=59.544
PRIMER_RIGHT_3_TM=59.968
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=0.4908
PRIMER_LEFT_4_PENALTY=0.253482
PRIMER_RIGHT_4_PENALTY=0.237277
PRIMER_LEFT_4_SEQUENCE=TGTACCTACCAATTCATCACCG
PRIMER_RIGHT_4_SEQUENCE=TGCAGAGCAAACTCAGACTGTT
PRIMER_LEFT_4=135,22
PRIMER_RIGHT_4=403,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=60.237
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_04372 Ps_contig_mira-and-tgicl-ass_franssen_33030
SEQUENCE=GGTTAAGAAAAAGGTTCTCTTTCAGACTTCATCTTATAGCACAATACCCTGTTGTTCAACATATTCTACAGTTCGTATATATGATAAAGAAAATTCAATATTACAACAGCAAAATCTTCTAACAAAGCATACATTTGTACCTACCAATTCATCACCGCCTTCTTCGGTTGCTGTAATCTTCGTCTTCACCTTCTTTATCCTTTAAGAAGCCAAGAAAAGCAAGAAACCCAAAAAAGAAAAATCCACCAGACCAATTGCCTCCACCTCCACCTCCTCCCCCGCCATCATCATCATCACGCGGCGGAAATCCAGATCCACCATCATCGATTTGTGGGGACTTCTCCTTACCGGGAAGATGAGTGATTGTCGGAGCCTCTCGAGTAACAGTCTGAGTTTGCTCTGCACCACATCTTGCATCCAAAGCTGCAGCACATATTATAGTTGCTGCCTGAGTAGAACGGGCTCTTCTATCAAATGGAAACTGTGTGCGACTCAATGATATTGAGTTATTAACATTCAGTAATCCTCTTGAAAATATAGATGTAGGAACAACGTTCTCTTCAATGCAGTTTCCACCTCTTCTCACTGGTGTTAATTTGGGCAAAGATGCAACTGCACTAGTGGTCAACATTGGTGTATGACAGATAACACACGGAGTTGTCGATGTGTTCGGATAAGGTGAGTTGTATGAACTGAAAAGTATGAAAAGGTTGCGAAATTGGGTTCAATTGTGGATTAATTTGAGGCTCCGAGAGAGAGAAAGTTGAAACCCTAACAATGGTTTGGAAGAAGAAGAAGAAAAAGAGGAAGACGATAGATAGACGAAGCAGGTTGGTCGCAAAATCTCAAGGACCTTGCAAATTGCCACCACCTCCCATATTT
TARGET=751,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2212
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.221115
PRIMER_LEFT_SEQUENCE=GAGTTGTCGATGTGTTCGGATA
PRIMER_RIGHT_SEQUENCE=GACCAACCTGCTTCGTCTATCT
PRIMER_LEFT=654,22
PRIMER_RIGHT=836,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=59.779
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.2514
PRIMER_LEFT_1_PENALTY=0.030276
PRIMER_RIGHT_1_PENALTY=0.221115
PRIMER_LEFT_1_SEQUENCE=TGTGTTCGGATAAGGTGAGTTG
PRIMER_RIGHT_1_SEQUENCE=GACCAACCTGCTTCGTCTATCT
PRIMER_LEFT_1=664,22
PRIMER_RIGHT_1=836,22
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=59.779
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.2985
PRIMER_LEFT_2_PENALTY=0.077340
PRIMER_RIGHT_2_PENALTY=0.221115
PRIMER_LEFT_2_SEQUENCE=GCAGTTTCCACCTCTTCTCACT
PRIMER_RIGHT_2_SEQUENCE=GACCAACCTGCTTCGTCTATCT
PRIMER_LEFT_2=565,22
PRIMER_RIGHT_2=836,22
PRIMER_LEFT_2_TM=59.923
PRIMER_RIGHT_2_TM=59.779
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.3008
PRIMER_LEFT_3_PENALTY=0.079714
PRIMER_RIGHT_3_PENALTY=0.221115
PRIMER_LEFT_3_SEQUENCE=ATAACACACGGAGTTGTCGATG
PRIMER_RIGHT_3_SEQUENCE=GACCAACCTGCTTCGTCTATCT
PRIMER_LEFT_3=644,22
PRIMER_RIGHT_3=836,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=59.779
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.4427
PRIMER_LEFT_4_PENALTY=0.221594
PRIMER_RIGHT_4_PENALTY=0.221115
PRIMER_LEFT_4_SEQUENCE=AGTTTCCACCTCTTCTCACTGG
PRIMER_RIGHT_4_SEQUENCE=GACCAACCTGCTTCGTCTATCT
PRIMER_LEFT_4=567,22
PRIMER_RIGHT_4=836,22
PRIMER_LEFT_4_TM=59.778
PRIMER_RIGHT_4_TM=59.779
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_04373 Ps_contig_mira-and-tgicl-ass_franssen_33030
SEQUENCE=GGTTAAGAAAAAGGTTCTCTTTCAGACTTCATCTTATAGCACAATACCCTGTTGTTCAACATATTCTACAGTTCGTATATATGATAAAGAAAATTCAATATTACAACAGCAAAATCTTCTAACAAAGCATACATTTGTACCTACCAATTCATCACCGCCTTCTTCGGTTGCTGTAATCTTCGTCTTCACCTTCTTTATCCTTTAAGAAGCCAAGAAAAGCAAGAAACCCAAAAAAGAAAAATCCACCAGACCAATTGCCTCCACCTCCACCTCCTCCCCCGCCATCATCATCATCACGCGGCGGAAATCCAGATCCACCATCATCGATTTGTGGGGACTTCTCCTTACCGGGAAGATGAGTGATTGTCGGAGCCTCTCGAGTAACAGTCTGAGTTTGCTCTGCACCACATCTTGCATCCAAAGCTGCAGCACATATTATAGTTGCTGCCTGAGTAGAACGGGCTCTTCTATCAAATGGAAACTGTGTGCGACTCAATGATATTGAGTTATTAACATTCAGTAATCCTCTTGAAAATATAGATGTAGGAACAACGTTCTCTTCAATGCAGTTTCCACCTCTTCTCACTGGTGTTAATTTGGGCAAAGATGCAACTGCACTAGTGGTCAACATTGGTGTATGACAGATAACACACGGAGTTGTCGATGTGTTCGGATAAGGTGAGTTGTATGAACTGAAAAGTATGAAAAGGTTGCGAAATTGGGTTCAATTGTGGATTAATTTGAGGCTCCGAGAGAGAGAAAGTTGAAACCCTAACAATGGTTTGGAAGAAGAAGAAGAAAAAGAGGAAGACGATAGATAGACGAAGCAGGTTGGTCGCAAAATCTCAAGGACCTTGCAAATTGCCACCACCTCCCATATTT
TARGET=786,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2212
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.221115
PRIMER_LEFT_SEQUENCE=GAGTTGTCGATGTGTTCGGATA
PRIMER_RIGHT_SEQUENCE=GACCAACCTGCTTCGTCTATCT
PRIMER_LEFT=654,22
PRIMER_RIGHT=836,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=59.779
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.2514
PRIMER_LEFT_1_PENALTY=0.030276
PRIMER_RIGHT_1_PENALTY=0.221115
PRIMER_LEFT_1_SEQUENCE=TGTGTTCGGATAAGGTGAGTTG
PRIMER_RIGHT_1_SEQUENCE=GACCAACCTGCTTCGTCTATCT
PRIMER_LEFT_1=664,22
PRIMER_RIGHT_1=836,22
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=59.779
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.2985
PRIMER_LEFT_2_PENALTY=0.077340
PRIMER_RIGHT_2_PENALTY=0.221115
PRIMER_LEFT_2_SEQUENCE=GCAGTTTCCACCTCTTCTCACT
PRIMER_RIGHT_2_SEQUENCE=GACCAACCTGCTTCGTCTATCT
PRIMER_LEFT_2=565,22
PRIMER_RIGHT_2=836,22
PRIMER_LEFT_2_TM=59.923
PRIMER_RIGHT_2_TM=59.779
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.3008
PRIMER_LEFT_3_PENALTY=0.079714
PRIMER_RIGHT_3_PENALTY=0.221115
PRIMER_LEFT_3_SEQUENCE=ATAACACACGGAGTTGTCGATG
PRIMER_RIGHT_3_SEQUENCE=GACCAACCTGCTTCGTCTATCT
PRIMER_LEFT_3=644,22
PRIMER_RIGHT_3=836,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=59.779
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.4427
PRIMER_LEFT_4_PENALTY=0.221594
PRIMER_RIGHT_4_PENALTY=0.221115
PRIMER_LEFT_4_SEQUENCE=AGTTTCCACCTCTTCTCACTGG
PRIMER_RIGHT_4_SEQUENCE=GACCAACCTGCTTCGTCTATCT
PRIMER_LEFT_4=567,22
PRIMER_RIGHT_4=836,22
PRIMER_LEFT_4_TM=59.778
PRIMER_RIGHT_4_TM=59.779
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_04374 Ps_contig_mira-and-tgicl-ass_franssen_33034
SEQUENCE=CAGTCCCAAAAGAAAGCTATCGAATGAATTCCAGTTTTCGCCTATGATTTCTAACCAGCTTGGTAGTGCACGGAGTATCAAGTTTAATGGGAATCTTGAAGAGCGTATACCCTCTAGTGCTACTTTGCAAGCCCTGGGAGGATGTAGGTTACACAAGAATGTATTACAGCAGGATAATGAAGCTTCTCGCATTTGGGCATCCTTGGYCTCGAATCGCACTTCCTTAGGGTATGATGTRTCACAAAAGGTTGCTTCTCAACCTAGTAGTAATAGCAACATGGACATAACTTCACTCCGATCTGGTGCAGTACATATGCAGAACTCGGGGAGGGATATTGATCTTAACTACACAAATATCAATGCTACATGCCAAGACARGCAAGGTAGTAGGAATACAGGTGCTGGAGTCTTCAGCAGGATGAATGGAGAATATTCATTTCCTTGCAAACATAATGGAATAGAGCCTCATCAAAATTTGAGGGGATCTTTGGATTTGCATTCTAATGAGACAATACCTGCCATGCATTTACTTAGCCTTATGGATGCCGGAGTGCAGTCACGTACACCCTTCAATGTTGGTGTTAATGCCCAAATGCTCAATAGACCCTCTTATCCTGGTGATTGCAATACCAAGATGGACATAAGTGCTTCTAAGGCCAATGGTACCCTGAAAAGGCAGTCATCTGATTATTACAACAGAAGCTACTTATCGGATAAATCCCATGGCTGTTTAATTGGCTCTCAAACCTTTGGCGGGTCTTCATCAGCTCAGCATGGAAAGAAGTTTACAAAAGATGCGGGATCCAATGATCAAAACGCAACAAAGTTTGGAAAGAAAGAAAAGATGAGGAGTTCCAACGCACCCCTGCAGAGTAGATTTCTTAAGCAGTGTAGCTTGTCTTATAACGAAACAAATACTTCCCAGCAACACAGATTGGAAGCTCACGGTACTCATACCTCTGTGCAATTAAAGATCACACCCGGTGTCTCATGTACAGTGAACAGAAATCCAGCTGAGTTCACCATTCCGGAAACAGGGAATGTCTATATGATCAGAGGAGAGGATCTCTAAAATTTTTTAATTAGTATTACCCCCAAAAACAAACTATTTTTTCCCTTA
TARGET=831,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04375 Ps_contig_mira-and-tgicl-ass_franssen_33037
SEQUENCE=CGNRGAGGTACTCGGGGAGGTTCCTTATCTAGAATGATAATTGTGGTTGTATCATCGTGCAGTTGAGTTCGTTCTGTCCTTCGTAAGCTTTAACATTTTTCAAAATCATCATTCAATCGAATTTAACAATGAACATGTTAGCATCATCATCATCAATCTCTCTTCAACCAAACGTGCTTCTTCTTCCCACCACTTCAATTCCATCGATTCGACCCATCAAAACCAACTACATCAAGATGAAAACAAACCGTTTTACTGCTTCTGCTGTTGCTGTTGCTGAAGATTCCGTTTCTTCACAGTCCTCTCCTTCTCTCCAGACACCGCCTCAGTCTCAGAAACTAGGAGTAGTTGTGAAACCAACAGATAAACCAAGACTCGTGTTGAAGTTCGTTTGG
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04376 Ps_contig_mira-and-tgicl-ass_franssen_33039
SEQUENCE=AAAGAGCACTNAAAAAAATNTGAACTCCATAACTTCAGAAAGAAAAATACATGGTGACTTTGAGTGTGTAGACCATAGAGATTGCTGAGTAAAATTGTAAATCCGGATGCTCTAATTAAAGACTTGTGTAATTGAAATATTTCCACGGTACTCTTTAAATTTCAATTGGCATTGGTTGACTAGAACTCCTAAAAACAGTTCCAATAGTTCCAAACTAATGTTTTGAGGCATCACAAAGAAAGAGTTCTTTTAAAAGGTTTCAATAACATAAAACATTAATAACTCTAACACCCCTTTCATAATTTTCTATGGTACAAGGCATTTTGTGAAAAAGCAATTGAATTCTATACCAGCTAATTTTAGGATGAAGATGCTAATTGCTTCAATTTCGCCTGTAAAATAATCCCAGCATCATCTGCCACATTCTTCTTTTTGGATGCAACCTCAACAAGTTGATCAAAGTTAGATTTGAGTAAATTGATTTTGCGCTCACAATAATCCTTTCCTTCAGGCATCGTTTTCTCGACGAAATAACCGGTTCCGATATCGACGAGAACGTGATGAGGATCCTGAAGAGTAGCGGGAACGTAGAGAGAAGCGGTGAGTGGAACGAGGATTTTACTGCCGTGAGATCGGAGAGAGAGATCGTTGAGAGCGGTTGTAGCGAGCTCAAGGCGAGTCGTGGCGGTGCCGATATTGGTGAGGCTGTCTTGAAGAAGGTTAACTTCCATATCTGCTTGTTCCTTAACTGCCTTCAACTGTTCGACGCTCATTCTCTCCAGAGTCATCGCTCCGCTGGCGCTCTTCGACGAAGCCATCTTTGAACTTTCTCTCTCTTTCTCGCTACTAGCTTCCTAAACCCTGCAACTCCCATTCGATTGCAGCCAACACCCCNTYNNYGACCAACATCTACT
TARGET=636,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04377 Ps_contig_mira-and-tgicl-ass_franssen_33040
SEQUENCE=AACGCAGAGTANGCGGGGGAAAGGGAAAAATAGTGGAACGTCTACCTGGTAATTCGGACACGATTCCTTTTTCATAAAAATTTCATTCTGAATCAAATGTCCTTTCTTCTTCTTCTATCATTTCACTATCTTGTTAATTCTTAGTTAATTGCGATGGAATCGAGTACAAACCCGGAATTCGATTATTTGTTCAAATTATTGATGATTGGAGACTCCGGAGTTGGCAAGAGTAGTCTCCTCCTCTGTTTCACCTCTGATTCTTTCGAAGATCTTTCTCCTACAATTGGTGTTGATTTTAAGGTCAAATATGTCACTATTGATGATAAAAAGTTGAAGCTTGCTATCTGGGATACAGCTGGTCAAGAGAGATTCAGAACACTAACAAGTTCTTATTATCGAGGCGCACAAGGGATCATTATGGCTTACGATGTTACTCGACGAGATACATTTACAAATCTCTCTGAAGTATGGGCTAAGGAAATAGACCTTTATTCAACAAATCAGGACTGCATCAAAATGCTTGTTGGAAACAAAGTGGATAAGGAGAGTGATAGAGTTGTGACAAAGAAAGAAGGAGTAGACTTTGCAAGAGAATACGGTTGCCTATTTATTGAATGCAGTGCTAAAACTCGAGTTAACGTACAACAGTGCTTTGAAGAGCTTGTTTTGAAGATTTTAGACACACCTAGCCTCATAGCCGAGGGTTCCAAAGGGGTCAAAAAGAACATTTTCAAGGACAAAGATCTCCAGTCTAATGGAGCCACAAGTGGTTGTTGCTAAGGGTGCTATTTAACTCAAAGTCAAAGAAAAAGGGTGTTGAATCCATTTTCAGAATCAGAGTTCTAACACATTTACTGAAGTCTTAAAACAACAAAGAATCCAGTCCCCTCCTTTATTTTTTCTGGAATATTTGTAATATAATGATCATGGTTCTTTTTTATTGTTATTATTTGCATTTAACATGTTGACGTTGAAGAATCTTGATATATCATGATATACAATTAAAGAACAAAGGGGGAAACTGGATTTCCTGCTTTATAGTGCATATTCTTGCTAGAGAAACACGACTTCCTT
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1241
PRIMER_LEFT_PENALTY=0.107763
PRIMER_RIGHT_PENALTY=0.016312
PRIMER_LEFT_SEQUENCE=GAACGTCTACCTGGTAATTCGG
PRIMER_RIGHT_SEQUENCE=GAGGAGGAGACTACTCTTGCCA
PRIMER_LEFT=35,22
PRIMER_RIGHT=242,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=60.016
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.1241
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.016312
PRIMER_LEFT_1_SEQUENCE=AACGTCTACCTGGTAATTCGGA
PRIMER_RIGHT_1_SEQUENCE=GAGGAGGAGACTACTCTTGCCA
PRIMER_LEFT_1=36,22
PRIMER_RIGHT_1=242,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.016
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.1241
PRIMER_LEFT_2_PENALTY=0.107763
PRIMER_RIGHT_2_PENALTY=0.016312
PRIMER_LEFT_2_SEQUENCE=GGAACGTCTACCTGGTAATTCG
PRIMER_RIGHT_2_SEQUENCE=GAGGAGGAGACTACTCTTGCCA
PRIMER_LEFT_2=34,22
PRIMER_RIGHT_2=242,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=60.016
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.3685
PRIMER_LEFT_3_PENALTY=0.107763
PRIMER_RIGHT_3_PENALTY=0.260719
PRIMER_LEFT_3_SEQUENCE=GAACGTCTACCTGGTAATTCGG
PRIMER_RIGHT_3_SEQUENCE=ATCAGAGGTGAAACAGAGGAGG
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=59.739
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.3685
PRIMER_LEFT_4_PENALTY=0.107763
PRIMER_RIGHT_4_PENALTY=0.260719
PRIMER_LEFT_4_SEQUENCE=AACGTCTACCTGGTAATTCGGA
PRIMER_RIGHT_4_SEQUENCE=ATCAGAGGTGAAACAGAGGAGG
PRIMER_LEFT_4=36,22
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=59.739
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_04378 Ps_contig_mira-and-tgicl-ass_franssen_33041
SEQUENCE=GTCGTTCCTACGAAAGGAATACAATAAAACTAAAATTTAAGTAAGAACAAAACTAACAAAGAAGTAACAAAGRAGAGTATGATATGAACAACCCCAACTAGTTACAAAATAAATAAATGAAAAAAGGCACACAGGAAGATTGACAAGAGCTTAAGTCTGATTCTATCAGTTATTTCAACTCTACCCAATCTATTGACGAATGAAAGCAAGTACCACACAAAAAATAATAATCAGCAGCAGTCTCTTCAGATAATAATCTTGTTGTCCTGGACCTGTGGGCAGAGGATATCTATGAACATGACCACCGTGAAATGAATTAGAAAACCCGTAAGGGAAACCAGCTGCTCCACCATACATGGCCCCGCCATGAAACCCATGCATCTGAAAGTTAAATAAAGATGGTATAAAACCACCGAATCCTGCAGAAAAAGTGAAATTTCCAAATCTTGTAGTTGCTGCAGGTGCAAATCCTCCCAATCCCCCCAACCCTCCTGCGAATCCAAACCCATGTTGAGAAAAAGGATTTGATTCAGGTGGAGGAGCAGTCTCAGGTCTCTGTCCGGCAGGACGATGAGGGATATTAACACCAGGAATAGACTTTGATCTCGGATCAGTTGACGTTTTTCCTCTCCCGTACAAAGGAACCAATTTCTCCTCCTCAACCATCGCCTTACACACCGGGCACTCTCGCGACTGCGAGTGAAAGTGAAGCCATTTGTAAAGACAAGGCCAGCAGAAAAGATGACCGCACAACGTAATTATTGGATCTTGTGCTAAGTCAAAGCAAATGTTGCATTCAAAATTGCCAGCATCACTGTTATTATTGTTGCTCGAATACGGAGGGCTTGGGGGTGACCTGCTTGTTGATTCCCCAAACCCACTTGCCATCTTAGAAACAAAAGGGTGAAGAAACGATAGATGGATCTTTGAAGTCAAAACCTCCCNCC
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04379 Ps_contig_mira-and-tgicl-ass_franssen_33045
SEQUENCE=AAGAAAGTAAGATACCAAATAATAATAAAAGGAAGATACCAAAACACATGTAATTTAATTAGCCTATGCATTTATAGCAAAGGAATCATAACCATTCATCCACACTATTTTTAATCAACTAATTTTAAAAGGGTAGAAGGAAGAAAAAAACTATATAATAAAACAAAGGAGAAAAAAAAACTTGCACAACACACAGCCAGCACACACACCAAATGAAGGTTGAGTATTACTACTATACCCCTCCATGTATGTTACAAAATATTGAGGGACCCATGAGAGATTACAATAAAGAGTAGGTTGAGCTAAATTCTTGTATGATTTTGAAGTGGGTAAGTTGTAATTTCAATGATGAAGGGCATGAGGAGGTGGTGCTGCAACCATAAGTGGTGGTGGAGGCTTGCATTGGTAGCACTTAGCAAAAAGCCCAAATGTGTCAATTTGTCTAATGAAGTTTGTCATACTTGCCCATCCTCCAAACCCAAACCCAACCACAAAAACCCATACCACAATAAATGCATTCAAAATATACATTGCTGTCCAACTCGGCATGAAAAACGGAGGCTTTTCAGCAGCATTCTGTCTCGCGGAGGCTTTTCGATAAGTGAGCATATGAGCTGCTGAAGGAATGATGTAGACAGTGAAGCTAACAAGAAGAGCTCCAACAGCAGAGTTAATGGGTCCAAAGAAAGGAAAAATAATGGCCAAAAACCAAATAGGTATCACCACTGGTAACCTTGCTAGTGCCCTTAAACAAATGCTTCTTGTGTCATGCATTCCAATCACTTTCTCCCATACAAAGTACAATGGAGTACAAGCAAAGCCAAATGTGATGAACTGGTGAATGAGCATGAGAACTACAGCACCATCACGAAATCCATTTTTGGGAAGAAGAGAGAAAGCATTGGAATGGTTAAGAAGTTCATCACCAAATGCCCAATAAACAGCAGAAGCAGAAGGAATCGTTAATGTGAAAACATATAAAGTTGCCAGCAAATATATGTATTTGAACTTTTGAGGCTTCCACATGGCGTGCATAATTTCTACTGTAACAGCATGTCCACCAAATGTGTATAGTATGTTAGTTGCTCCAGTGAAATATAGCACTAACTTTTTTGGACCACTATGTGTCACATTTTCTGCCTGTCCATGAACAATAGCTGCTATAGTAAGATACCAAGCAGTGTAAGTGGTCATTCCAAGTCCAAGAAAAGACCAAATACGATAATTATGAAACGATGGTATGAACACAGTTGTCGCACAACATGCTCCAAATATGTAAGTCCAAGTTCTTTTGTCCAAGTTGTCATTTATGTAGTAGATGTTGCTTGCACAAGCTATAAGCTGAATCACAGATCCAAAGAGAAGGAAAGTACAGTTGAAAGCTAGACCCAATGCTTTCCAAAATGGACCCAGTAATCCATCAAGCACTTCAAACCACTGAATAACATGGTTCTTGAAATTAACATTTTCCTTTTCTTTTCTAGTTCGGTACTCAACATATAGAACACTGATGAGATAAGCAGTCCAGCTTCCAAGAATTCCATAGAATATTTGAAGCAAGATTCCAGATAGCATGCCAAGTTGAGAGAATGAATATGGCAGTGTCAACAAAACTTGAGCCACTTGATTTGAAGCACAACTGAACCATGCATCCCAAACAGAACCACCATGCCAGAGAAAATTCTTGACACTGAATGAATGATCTTGTTCTTGCTCTTCTTCCTTTCCAAGTACTGATTCTTGTTGATCTGTCTCATTGAAACTGCTGCTAACTATTGCTTCCTCGGCTTGTTTCTGAGCCAACATTGTTCCGTCTTCAAACCTCTATGGATCAGAAATTTTCGAAGAACCAAGCTTTATACTAGTTGAATCTCTTTCTAAATACAAAGCAACCCTTTATTTAAGAAGGAAAGAGCTTGAGTAATTGAATGTGGAGAACCAATTTCAGTAAAGTGGCAGAGAGATTAATGGCTATAGAATTCTCTTTTGATGTATACAAACAAAGCTTTAAATGCGATATTGGAGGTTCACTGCAAGGATGAAGAGAAGGAAAACAGAGAGAGAGTAATAAATGGTACG
TARGET=2056,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04380 Ps_contig_mira-and-tgicl-ass_franssen_33052
SEQUENCE=TAGCATCTGAGCCATGTTACAGCAGCCAATGAACTGGTCACTTTACATGTCAACGGTGCACTTCTACCTCGATACATGCTCCTCGAACCGAAACTATGGCTTGCAGAATTATTGAAGAATCCGATACTCCTGCTACTGAATTTCTTTGGTTTTCTCGAACCACCGTTGGCCAAATCATTATCTCCTCCGGATCTTGAATTGACGCAGAAATCAGGCAGTGATCTTGAGGTGACGTTATCTTCAACTCCCATAACGAACTGTCCTGCCGTGAGATTCGCTAAAGCCCATGCGGCGGATAAGTAATCACATTTCCATAATTTGATTGCAGAAATTTCTCCAATGATCTTACACCGACCGTCTGATGTGGTTTCGATTACTGCAACTGCACTTGGATCTTGAGGAACCTTTTGAGATGATTCGAGCGCGGGAGTGGAGGATTCGATATAGCTGTAGTTTGGATTAATCGCTCCGAGTGCAGCGATGGTAGTGAGAGGAATCGGAGCTAGAGCTCCAAGACAACCGATGATGAAACGGAGTACGTCTTCTCTGGATAGACAACAGTACTTATCACGGATGGATGCTGAGGTGTTCCGATTTAGATTCGCAGGTAGATTGTTGCTGCTGCTGCTAGGAGATTCAGGATTCTTGAGCCACTTGCCATAGTAGATTACTGAGAAACGCTTACTCATGCCCTTCCATACTATACTCTTTGGAACAAGCAAACGTTTCACGCCTTGCTTCATCATGTCCAGTGCATCTATCAACCTTGTTCCAGGATCAACCAATCTAAGCAAAGAATTATTGGAAAGAACAACCTCAGAAACAGGTGTCTTCATAGCCTTATCTTGATCCTCCAAACACCGAGTCTTAGCCAAGAACGCAACAATATCAAACGAACTAAGAATCCCAACAAACCTCATCTGTCTCATATCACTATTCTCAATCACACCTTGAGATCTCTTTTTCCAAACCGGTATACTCCCTTCAGGCGACTCCGCTATTGCTCTAATCGCAGATTCCACCGTCTCCGTTTCATGAAACTCCACCAACTCCGGTTTCCCCACCGTCAGATCTCCCACCACATGATAAACCAACACCGACGCCATTAAAACCCAAAAAAAAAAATCAATTTTTTCCCACAGAATCGAGAAAAATTGATGGGGTGAAAGGAGAATGGAATAAAAATTGATGGCTAATAAAAGAGTATAGAAAAAAGATGAGGAAG
TARGET=617,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0170
PRIMER_LEFT_PENALTY=0.010077
PRIMER_RIGHT_PENALTY=0.006951
PRIMER_LEFT_SEQUENCE=GTAGTTTGGATTAATCGCTCCG
PRIMER_RIGHT_SEQUENCE=GTATGGAAGGGCATGAGTAAGC
PRIMER_LEFT=449,22
PRIMER_RIGHT=700,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.0254
PRIMER_LEFT_1_PENALTY=0.018437
PRIMER_RIGHT_1_PENALTY=0.006951
PRIMER_LEFT_1_SEQUENCE=TACTTATCACGGATGGATGCTG
PRIMER_RIGHT_1_SEQUENCE=GTATGGAAGGGCATGAGTAAGC
PRIMER_LEFT_1=561,22
PRIMER_RIGHT_1=700,22
PRIMER_LEFT_1_TM=59.982
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=140
PRIMER_PAIR_PENALTY_2=0.1000
PRIMER_LEFT_2_PENALTY=0.093026
PRIMER_RIGHT_2_PENALTY=0.006951
PRIMER_LEFT_2_SEQUENCE=CTAGAGCTCCAAGACAACCGAT
PRIMER_RIGHT_2_SEQUENCE=GTATGGAAGGGCATGAGTAAGC
PRIMER_LEFT_2=502,22
PRIMER_RIGHT_2=700,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.8000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=0.1056
PRIMER_LEFT_3_PENALTY=0.010077
PRIMER_RIGHT_3_PENALTY=0.095538
PRIMER_LEFT_3_SEQUENCE=GTAGTTTGGATTAATCGCTCCG
PRIMER_RIGHT_3_SEQUENCE=TCTACTATGGCAAGTGGCTCAA
PRIMER_LEFT_3=449,22
PRIMER_RIGHT_3=666,22
PRIMER_LEFT_3_TM=59.990
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.1056
PRIMER_LEFT_4_PENALTY=0.010077
PRIMER_RIGHT_4_PENALTY=0.095538
PRIMER_LEFT_4_SEQUENCE=GTAGTTTGGATTAATCGCTCCG
PRIMER_RIGHT_4_SEQUENCE=TACTATGGCAAGTGGCTCAAGA
PRIMER_LEFT_4=449,22
PRIMER_RIGHT_4=664,22
PRIMER_LEFT_4_TM=59.990
PRIMER_RIGHT_4_TM=59.904
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_04381 Ps_contig_mira-and-tgicl-ass_franssen_33053
SEQUENCE=AGGAAATANNTATAAGAGAAAAGAAAAGCAAAAGTGCGAAACTAAGAAAGAAAGATGTTCAACATATTTAACCGAGGCAGGGACAATTCCGGCGATGCCTCCCCGCCTACTCCGTCGGCGAGGCCGTCATCTTCGGCTTCTCAGTCCACCGGTCCCCCGAGGCCTATACGTCTTGTCTACTGCGATGAAAAAGGAAAGTTCCAGATGGATCCGGAAGCCGTCGCAGCGCTTCAACTTGTCAAGGAGCCCATCGGTGTTGTTTCCGTTTGTGGTCGTGCTCGACAGGGAAAGAGTTATATCTTGAATCAGCTCTTAGGAAGGAGTAGTGGATTTCAGGTAGCATCTACACATCGACCCTGTACTAAAGGACTTTGGTTGTGGAGTTCACCTTTGAAGAGGACTGCTCTTGATGGAACAGAATACAATCTTCTGCTATTAGACAGTGAAGGAATAGATGCTTACGATCAAACGGGAACATACAGCACCCAGATATTCTCCCTGGCTGTTCTTTTGTCTAGCATGTTCATTTATAATCAGATGGGTGGTATAGATGAGGCTGCCCTTGATCGTCTTTCTTTAGTCACTCAAATGACAAAGCACATTCGTGTTAGAGCATCTGGAGGAAAGACTTCTGCGTCTGAAATAGGACAATTTTCCCCAATTTTTGTTTGGCTATTACGGGACTTCTATTTGGACTTAACTGAGGATAACAGAAAAATAACACCCCGAGACTATCTGGAACTTGCTTTGAGGTCTGTTCAAGGGAACAAAAAAAGATATAACTGCCAAAAATAGAGATTCGAGATTCTATTCGTGCTTTATTTCCCAGATAGGGAATGCTTCACCCTTGTAAGACCTTTGAACAATGAAAATAGATCTACAGAGACTGGACCAGATATCATTGGACAAACTAAAGAACCTGAATTTACGTAAAAACGGGGACGTTGTATGAATTTAGTTTAAAGTTTTAGTTTTGTAGAGAACAA
TARGET=44,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.4124
PRIMER_LEFT_PENALTY=4.310268
PRIMER_RIGHT_PENALTY=0.102139
PRIMER_LEFT_SEQUENCE=GAAAAGCAAAAGTGCGAAAC
PRIMER_RIGHT_SEQUENCE=CTGGAACTTTCCTTTTTCATCG
PRIMER_LEFT=22,20
PRIMER_RIGHT=203,22
PRIMER_LEFT_TM=57.690
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=4.4275
PRIMER_LEFT_1_PENALTY=4.310268
PRIMER_RIGHT_1_PENALTY=0.117281
PRIMER_LEFT_1_SEQUENCE=GAAAAGCAAAAGTGCGAAAC
PRIMER_RIGHT_1_SEQUENCE=TCCTTTTTCATCGCAGTAGACA
PRIMER_LEFT_1=22,20
PRIMER_RIGHT_1=194,22
PRIMER_LEFT_1_TM=57.690
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=4.5470
PRIMER_LEFT_2_PENALTY=4.310268
PRIMER_RIGHT_2_PENALTY=0.236697
PRIMER_LEFT_2_SEQUENCE=GAAAAGCAAAAGTGCGAAAC
PRIMER_RIGHT_2_SEQUENCE=GAACTTTCCTTTTTCATCGCAG
PRIMER_LEFT_2=22,20
PRIMER_RIGHT_2=200,22
PRIMER_LEFT_2_TM=57.690
PRIMER_RIGHT_2_TM=60.237
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=4.7507
PRIMER_LEFT_3_PENALTY=4.310268
PRIMER_RIGHT_3_PENALTY=0.440473
PRIMER_LEFT_3_SEQUENCE=GAAAAGCAAAAGTGCGAAAC
PRIMER_RIGHT_3_SEQUENCE=TTTCATCGCAGTAGACAAGACG
PRIMER_LEFT_3=22,20
PRIMER_RIGHT_3=189,22
PRIMER_LEFT_3_TM=57.690
PRIMER_RIGHT_3_TM=60.440
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=4.9084
PRIMER_LEFT_4_PENALTY=4.310268
PRIMER_RIGHT_4_PENALTY=0.598109
PRIMER_LEFT_4_SEQUENCE=GAAAAGCAAAAGTGCGAAAC
PRIMER_RIGHT_4_SEQUENCE=CCTTTTTCATCGCAGTAGACAA
PRIMER_LEFT_4=22,20
PRIMER_RIGHT_4=193,22
PRIMER_LEFT_4_TM=57.690
PRIMER_RIGHT_4_TM=59.402
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_04382 Ps_contig_mira-and-tgicl-ass_franssen_33071
SEQUENCE=GGAAGTTGTAAAGGAATTAAACACAAGGTGTGTGAGTGAATAAAACTATAGTCTTGAAACATCACAACATAGAGTAAACATTGTAAAAAAAATGACNTNTACTAACAAGTATCAAAAGTGCATAATTTATCCACCAAATTATTCATTGTCACAAATTTAGTAAAACTAATAAATAAAAGACCAACAAATATATAACTGTAAAAATCTCACAAAACAATCTATGCAAACAAAACCACACTATCTAACAGTCTAAAACCTCTAAAAATTTAAGCACTTTTTTGATAGATCTTCATGATTGTCTTCAAGCTATTGTACTTCATTGATCATTATTTGTATCTGGTTGATTGATGACAACTGAAAACCTTACTTGGAAAGAACTTCTCCTTCCCCTAAATTTAACTGTAGATTTGCTGCAGCGTTCGAAGAAATTCAACCCCCCTTCTTGTACTCGACAATATCGACACAGACACTTATGACGCTTTAAGGATTGTGCTCAAAAGAGCTTTTACACTATGGTTCCAACATAACCAAATCCTACAATTGTGAAGGTTAAGGTTTGAAGGAAGAGGTAAATAAAGTAGTTGTGTTTTCCATGCACATAATCATAGCATCCACAGAAGAAGAGAAAGACTGCAAATCCCAGCTCCAACAAATTTAGCCTTTCCATAAATTTGGATCTTGTTTTCTTTTGAACTTTGACATGGCCCTTCTTGGCTGCAGCATCAGATTTGAGTTTATTGTTGTTAACGGAATCTCCGAGTTTTTCGGTTACAACCCATTCGTTGGCCCTTCCGTATTCTAACAAACCGATAAGGGTCGCCTTGGTGCGATGCAGAGACATGACATTCTCGAAAAGGATCCAATAGAACAAAAGGTGAATTGACCTTGGTGTTCCGACGGAGTTGAGAATGGTGATGATGGAAGGAATATAAACCGCTCCCCAAATTGGAACATAAACCTCGGGAACCAAAATCGTGAGAGGAATTACGAGACAGTAGAAGAAGAAAGTGACCATGTGAGCTACGATTTTACGAACAAGGAAAAAGCTGTATATGACATACACTTTCTTCCAAAACTTCACTTTCTTGTTCCTTATAATATCCATTGCCATTTTGCGGAACAGATTAGCCGGACCACACGACCATCGATGCTGCTGGAATCTGAAGGCCCTCAAAGTACTCGGAAGTTCGCTGTTTGCCTGGAGATCACCAAGGTACAAAAATTTCCATCCCCTAAGACTTGCTCGGACAGCGAGATCCATATCTTCGACTGTTGTTCTGTCTTTCCACCCTCCCGCTTCGTTGATAGCAGCTATTCTCCAAATACCAGCAGTGCCATTGAAACCAAAGAAAGCATGAGTAGCTGAACCAACTTCTTGCTCAACTGTGAAATGATAATCCAAAGACATCTCTTGCATTCTTGTCAACAAACACTCATTAGCATTCACAAATCGCCAACGACCTTGAACAAGAGCAAGTTCAGGATTTCCAACAAGAAAAGGAATGCCTCTTCTGAGAAAATCAGGTGGTGGACTAAAATCAGCATCGAAAATAACAACATATTCACAATGTTTAACATAGCTACGTTTTAATCCTTCTTTCAATGCACCAGCTTTGTAACCAACTCTGTTTTCTCTTATTTGGTATGTTATATTTATTCCTTTACTCGCCCATCGTTGGCATTCCATCTCCACCATTTGCTTAACAACAGGGTCCGTAGAATCGTCGAGAACTTGGATCACGAGTCGATCCGTAGGCCACGAAAGGCCACAAGCAGCTCCAATCGAAACCTTGTACACCTCTCTTTCATTGAACATTGGAATTTGAACAAGAACAACAGGGAAATTAGAGCCACCGAGTTCTTCATCGTCTTGAAGAGGCTCATAGTTATAACGTTGTTCAGGTTTCTTCCAGAAGAGTTTAATCAAAACAATAACAATACCCATATAAACCCTCTCCATGAAAAGCATAAGAGCCATTGCTAATGAAATGTAAACACAAGCATTCAAAAAAGGAACAATCAATGGTGCTTTCATCACATTCCAAATCATCTTAAATTGACTTGACACATCATAGTTCACCTGAAATGACTCTGGTATATAAAATTTAGGTTCTGATTCAACCATTTTTTTCACTCTAAAAAATCAAATTCAACCAAAAAAAAATGCAAGATCTCTCTCTGTTTCTTTCTCTTTACACTGTCAAAAATAAAATAAAAAATACAAAACTTGTTCAGAATTTTAGGAACAAAAGAATGCCTTCAGAATAGAGGCATTCTTTCTAGAGTGAGAGTGTAAGAGAGAGAGAAGGGCCCT
TARGET=2296,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04383 Ps_contig_mira-and-tgicl-ass_franssen_33083
SEQUENCE=TTTCTTTTTTTTTTCTTTTCTTTTTTCTTTCTTTTCCCTTTTCTTCTTCTTCTTCTTTTCTTTTCTCTTCTTTTTCAATTTTGACTCTCCTTTGCTTCTCCTTTGCTTCTCCTACCTCTGTAACCCTAACCCTAGAAACTGAATATGCTACCACAAAAGCTTAGACAGAATCCAATTATGCAACACTCTCTCTACCATCACCCAGCTCTTATCACTCCTCCTCAGATAGAGCCTATCTTGAGTGGAAATCTGCCTCCTGGCTTTGATTCAAGTACATGCCGCAGTGTCTATGTAGGCAACATTCATCCTCAAGTTACAGAAGCACTTCTTCAAGAGCTTTTTTCAAATGCTGGCGCGCTTGAAGGTTGCAAGCTTATTCGGAAAGAGAAGTCATCATATGGATTTGTCGACTACTTTGATCGCAGTTCAGCTGCTATTGCTATTGTTACTCTCAACGGAAGGAATATTTTTGGACAGGCTATCAAAGTTAACTGGGCTTATGCTCGTGGCCAGAGAGAAGACACTTCAGGACACTTTCATATTTTTGTTGGTGACCTTAGTCCCGAGGTTACAGATGCAACTCTGTATGCTTGCTTCTCTGTGTATTCTAGTTGCTCAGATGCAAGGGTAATGTGGGATCAGAAGACAGGCAGATCCAGGGGATTTGGATTTGTTTCTTTTCGAAATCAGCAGGAAGCGCAAAGTGCCATAAATGAGTTAACTGGTAAATGGCTTGGCAGTAGACAGATTCGTTGTAATTGGGCCACGAAAGGAGCCAATACCAATGATGAAAAGCAGAGTTCAGATTGCAAAGTTGTTGTGGAGTTAGCTAGTGGAGTTTCTGGGGAGATCACAGAAGAACGGCAAGAAATTACCAGTTATGACGGTCCAGAGAAGAATCCCCAATACACCACTGTTTATGTTGGCAATCTTGCTCCAGAGGTTACTTCTGTCGATCTCCATCATCATTTTCACGCCCTCGGTGTTGGAACTATTGAAGATGTTAGGGTGCAACGGGATAAAGGTTTTGGGTTTGTGAGATACAGTACCCATGGTGAAGCAGCTCTTGCTATACAGATGGGTAACACTCGGTTTCTGTTCGGTAAACCTATCAAGTGTTCATGGGGTAGCAAGCCAACTCCTCCGGGAACAGCCTCTACTCCTCTTCCTCCACCAGCTTCTCCGCATGTACCAGTGCCAGGTTTTTCACTTGCCGGTCTTGCAGCGTATGAACGCCAGATGGCTTTGAGCAAAATGAACGGTGCACACGCCCTTCTGCAACAGCAGCAGAATCAGCATCGCCCTTAAGCGAGGCCACGACCRAGTGGGGTAAGTGGGTCGCCTTCCGTAGGGAGTCGGTTAGGG
TARGET=41,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04384 Ps_contig_mira-and-tgicl-ass_franssen_33088
SEQUENCE=ACCTAAATAATTTRAATATAAATAAATCAACTTGTCATATAGCATAGTTGACAATGTAGTCAGTCAAATTTGTCTGACGGAAAACGATTGTTTTTAATTTACTACTGCTTTTGAACTTGACTGCAAAATTTGAAAGAACCACAACCTATTAAAATACATAATCATCTTGCTTATGGAAAGCATGGAAATTTGAGTATGGCTTCATCCATAGCTGTTTCTTCGCAATCTTTTGATGGCACTCTCAAGCACAGGACATACCCTTGACTGCTCATTATAGTAGTCTTTAAGCACGCCGAGATACAATGGCTTCTTTTCCTTTCTCAGCTATCTTGAGGGTTTTACTAAATTCTTGTGTTCTCGTGGATTGGATAGGAGGTGGATCAAAATTAACCGAACGCACAGACATCTTAGCGATCTCAGTGATTGCATCATCTCTAACACCAGGAACATCGCTCGTCAAATCTTCATAAGCAGCCTCAAAAGCCTCTTGCCAAAACCTCGGCAACCTGATCCTACGGCTGTTGGTATACACATCCCACATATGCCTCACCACCTTCTCCCTCTCCTCCATTTCAAGAACATCTAGGTCATCGTGAATTGGAGTATCTAAATCACTGGATAACGAATTGAGATTACCAGACCAACGAAGAGAAACGGTACCGGTGAACATGGACCAAAAAGCGAGAAGCAAAATAGCAGCTAATGCCCAGAATTTGTACCGTCCTTTCCCAAAAATAGACGAATCCATGTTTTCCTTTTTGCTACTTGTTGTTGTTGAGGTTGGAAGTCCATCGTCATCCTTCATTTCTCGTTTGGATTTCTCGAAATCGATCTAATCGATGTGTTTCGCGTCGCACACAATCAATTAGATTTAACAACGGTTGATAAATAATCGTTATGATTCTATTTCTGTTGTGGTTTCTTTCTACACCGGTTGAAGCTTCATTGTTTTGTTTTCCGATCTAATTGTGTTGTTGTGTGAATGAATGTCGAATGTGAGACACCACCGGCACCGCGTTCTGATTACCCCCG
TARGET=766,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04385 Ps_contig_mira-and-tgicl-ass_franssen_33090
SEQUENCE=AGGGACTTCCCCCACTTCAACGTGCTCTTGCCTACTCTATTATTGCCATAAAGCTATCAAATCAGTGAACGCTTAGAGAGAGAAAGAGAGAAAGAGGATTTTAGAGAGACAAAATTCATGGAAGTTGCAGGTCTGAAGAACGAAGAGAAGATGGTGTTTGATGAAACTGAACTGAGGTTGGGATTGCCTGGAAATGGAAGCAAAGTAACAGAAGTTGTAGTAAGAAAGAGAGGTTTCAGTGAAACTGAGAGTGAGAGTGAGAGTGAAAGCGAAATAACCACTGTGGATTTGAAGCTTAATCTCTCTCCCAAAGAAGGTGCAGCAGATCCACAGCTTAAACCTAAGGAGAAGTCTCTTCTAGTTTCTGATTCCGGCACCAAGCCTCCTGCCAAGGCGCAAGTGGTGGGATGGCCACCGGTGAGGTCGTTTAGGAAGAACATGTTTGCAGGCCAAAAGAGCATAGGGGGATCAGAAGAAACTACGAAGAAAAGCAGTGATGGTTCTAATGCAATAAGCTTTGTGAAAGTTAGCATGGATGGAGCACCTTATCTTCGCAAAATAGACCTAAAAATGTACAAGAGTTACCCAGAGCTTTCTGATGCCTTAGCCAAAATGTTTAACTCCATCACCAATGGAAATTGTGAATCCCAAGGAATTAAGGATTTCATGAAGGAGAGTAACAAGATGATGGATCTATTGAATACCTCTGACTATGTTCCAACTTATGAAGACAAAGATGGTGATTGGATGCTTGTCGGTGATGTACCATGGGAGATGTTTATGGAATCATGCAAACGTTTACGTATCATGAAAGGAAAGGAAGCTATTGGACTAGCACCAAGAGCCATGGAAAAATGCAAGAACAGAAGCTAGCTAGACTAGTCTTTACAGTCCATAACCTGCTCCATCCTCAGTGCTACTACATGGCCTAAGAAGAAGGTTGTTGAAAATATATAGATAATTTATGCTTGTGTGTGTGTAACATTATGGTTAATTTGTTATATTATGGTACTATTTCAAACAAATTAAGCTTTGAGATGTTTTTTAGTTATGAACAGAAACGATAATCACTTTAAATTATGTGTTTCTCTTGCAAGATTTTTTTTATATTTAATTGTTGTGTTGTTTGCAGTAAATTTAAATAAACAAACAATAATA
TARGET=970,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04386 Ps_contig_mira-and-tgicl-ass_franssen_33092
SEQUENCE=CCTATTAAAAGAAATTGCAAATTCAAAATTCCTTATCTAACATATAATATATATAGTGTTCATTATAGAATATACATATGAATAAGAATGCATGAATATGAATATATAACTGCAGAGACTAATCAAAGCTCTGAATTCAAATCCATGACATCTACCGTAACTAACCCACCAGAAAACAAAAAGGAAAAATTTACATTATCTGGTTTCTAACTTATTTTCTTCATACACGCTTATCATTTCCGAGCTCACAAGCTGTGCTGCCTTGGCTATACCCAAGTCATTTGATAACTTCTTGCATAATTGTCTATATACGGTTGCATCTCCATAAGATTTCCGTAGTTCGACTACATACAATGACGGACCTATTTCAAAAACCTCTGCCGCAACCGAGAGGCTTCCTAGACTTTTATGTGCTTTATTCTCGTGTATAACTTTCAATTTTCCATTTTTTTTCTGAACACTAAAACCCATTTCTATTACAATGTCCTCAATCTTCTCCGTCAAATCTTTGGGTGAATGGTTTGATGTAAACCTTATCTTTCTCTCCGAGACATCCTCTTTCTCGAAAAAGCCAGAGAGGTCTAAACACGAAGACATCCCTATCAACTGAAATGCATTGATAAGCTTCGGCGATTTTGGACTAGCCAGGTCTGCTTCACTGGACACTTCTTGGATGGGAAAGGCTTCATCGCCGATGTATACATCATCTTCCTCGTCGTCTCGGTTTGCAGGACTATAACCCTGTTTAAACCATTCATCTTCTTTGATCATAGACATTGTTATTCTGGTTTCGGGATTGGGATCAAGAATTCTCTTTATGATGTTTCGAGCGCCGGGCGACAACCATATCGGTATTTGAACGTCTCCTTTGAAAATCTTTTGATATAGAACCGCGAGATTTCTGTCGTCGAAAGGAAGGTATCCTGTTAGAATTACGTATAAGATAACACCGCATGACCAAATATCCGATTTCGCTCCGTCGTAGCCTCTATTCGCAAGAATCTCGGGAGCAACATAATTTGGACTTCCACAAGTTGTATGTAGCAACCCATCTGCCCTACAATGTTGCGGCAGAGCACTGAGGTTAAAATCAGTTATCTTTATGTTCCCTTTGGCATCCACAAGTACATTCTCTAACTTAAGATCTCTATGGAAGACACCTTTATTGTGGCAGCAGCTCACACCATCAATCAATTGTTGGAACAACTTCCTACCATGAGCTTCGGTTAGTTTACCTTTTGACGCAATTTTGTCAAATAACTCTCCTCCATTCACATACTCAAGTACCATATAAATTTTGGTTTTGCTTGCCAACACCTCGTACAACCGAACAACGTTGGGATGTTTCAAAAGCTTCAACGTTGATATCTCCCTTTTTATCTGGTCAGTGTTGTTGAGATCAACAATCTTATTCTTTTCGAGAATCTTAACAGCAAAAAAGTGTCCCGAATCAGTGTTTCTTGCTAACTTGACTTTTCCGAAATTTCCTTCACCAAGTGTTTTACCCAATTCGTATTTCCCCAATCGCATTACTTTGAATTCTTTCTTCCTCTCTTGGTTTATTATCACCATTCCCAAACACAACCTCTTTGATTTCTCTCTCTCCTCTCTCTCTCTCTCTAGAAGACTTCTTTCTTCTATATTGTGTAGTTAGTTAAGTAGAAGTAATAACTACATGGTAGGGTTAGGAAAGACGACCGAACGG
TARGET=1596,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3279
PRIMER_LEFT_PENALTY=0.093026
PRIMER_RIGHT_PENALTY=0.234845
PRIMER_LEFT_SEQUENCE=AAAAAGTGTCCCGAATCAGTGT
PRIMER_RIGHT_SEQUENCE=GGTCGTCTTTCCTAACCCTACC
PRIMER_LEFT=1435,22
PRIMER_RIGHT=1698,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.235
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.3279
PRIMER_LEFT_1_PENALTY=0.093026
PRIMER_RIGHT_1_PENALTY=0.234845
PRIMER_LEFT_1_SEQUENCE=AAAAGTGTCCCGAATCAGTGTT
PRIMER_RIGHT_1_SEQUENCE=GGTCGTCTTTCCTAACCCTACC
PRIMER_LEFT_1=1436,22
PRIMER_RIGHT_1=1698,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=60.235
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.3771
PRIMER_LEFT_2_PENALTY=0.142283
PRIMER_RIGHT_2_PENALTY=0.234845
PRIMER_LEFT_2_SEQUENCE=CCAAGTGTTTTACCCAATTCGT
PRIMER_RIGHT_2_SEQUENCE=GGTCGTCTTTCCTAACCCTACC
PRIMER_LEFT_2=1492,22
PRIMER_RIGHT_2=1698,22
PRIMER_LEFT_2_TM=60.142
PRIMER_RIGHT_2_TM=60.235
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.4893
PRIMER_LEFT_3_PENALTY=0.254467
PRIMER_RIGHT_3_PENALTY=0.234845
PRIMER_LEFT_3_SEQUENCE=TCCTTCACCAAGTGTTTTACCC
PRIMER_RIGHT_3_SEQUENCE=GGTCGTCTTTCCTAACCCTACC
PRIMER_LEFT_3=1485,22
PRIMER_RIGHT_3=1698,22
PRIMER_LEFT_3_TM=60.254
PRIMER_RIGHT_3_TM=60.235
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.4893
PRIMER_LEFT_4_PENALTY=0.254467
PRIMER_RIGHT_4_PENALTY=0.234845
PRIMER_LEFT_4_SEQUENCE=CATTCCCAAACACAACCTCTTT
PRIMER_RIGHT_4_SEQUENCE=GGTCGTCTTTCCTAACCCTACC
PRIMER_LEFT_4=1569,22
PRIMER_RIGHT_4=1698,22
PRIMER_LEFT_4_TM=60.254
PRIMER_RIGHT_4_TM=60.235
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_04387 Ps_contig_mira-and-tgicl-ass_franssen_33092
SEQUENCE=CCTATTAAAAGAAATTGCAAATTCAAAATTCCTTATCTAACATATAATATATATAGTGTTCATTATAGAATATACATATGAATAAGAATGCATGAATATGAATATATAACTGCAGAGACTAATCAAAGCTCTGAATTCAAATCCATGACATCTACCGTAACTAACCCACCAGAAAACAAAAAGGAAAAATTTACATTATCTGGTTTCTAACTTATTTTCTTCATACACGCTTATCATTTCCGAGCTCACAAGCTGTGCTGCCTTGGCTATACCCAAGTCATTTGATAACTTCTTGCATAATTGTCTATATACGGTTGCATCTCCATAAGATTTCCGTAGTTCGACTACATACAATGACGGACCTATTTCAAAAACCTCTGCCGCAACCGAGAGGCTTCCTAGACTTTTATGTGCTTTATTCTCGTGTATAACTTTCAATTTTCCATTTTTTTTCTGAACACTAAAACCCATTTCTATTACAATGTCCTCAATCTTCTCCGTCAAATCTTTGGGTGAATGGTTTGATGTAAACCTTATCTTTCTCTCCGAGACATCCTCTTTCTCGAAAAAGCCAGAGAGGTCTAAACACGAAGACATCCCTATCAACTGAAATGCATTGATAAGCTTCGGCGATTTTGGACTAGCCAGGTCTGCTTCACTGGACACTTCTTGGATGGGAAAGGCTTCATCGCCGATGTATACATCATCTTCCTCGTCGTCTCGGTTTGCAGGACTATAACCCTGTTTAAACCATTCATCTTCTTTGATCATAGACATTGTTATTCTGGTTTCGGGATTGGGATCAAGAATTCTCTTTATGATGTTTCGAGCGCCGGGCGACAACCATATCGGTATTTGAACGTCTCCTTTGAAAATCTTTTGATATAGAACCGCGAGATTTCTGTCGTCGAAAGGAAGGTATCCTGTTAGAATTACGTATAAGATAACACCGCATGACCAAATATCCGATTTCGCTCCGTCGTAGCCTCTATTCGCAAGAATCTCGGGAGCAACATAATTTGGACTTCCACAAGTTGTATGTAGCAACCCATCTGCCCTACAATGTTGCGGCAGAGCACTGAGGTTAAAATCAGTTATCTTTATGTTCCCTTTGGCATCCACAAGTACATTCTCTAACTTAAGATCTCTATGGAAGACACCTTTATTGTGGCAGCAGCTCACACCATCAATCAATTGTTGGAACAACTTCCTACCATGAGCTTCGGTTAGTTTACCTTTTGACGCAATTTTGTCAAATAACTCTCCTCCATTCACATACTCAAGTACCATATAAATTTTGGTTTTGCTTGCCAACACCTCGTACAACCGAACAACGTTGGGATGTTTCAAAAGCTTCAACGTTGATATCTCCCTTTTTATCTGGTCAGTGTTGTTGAGATCAACAATCTTATTCTTTTCGAGAATCTTAACAGCAAAAAAGTGTCCCGAATCAGTGTTTCTTGCTAACTTGACTTTTCCGAAATTTCCTTCACCAAGTGTTTTACCCAATTCGTATTTCCCCAATCGCATTACTTTGAATTCTTTCTTCCTCTCTTGGTTTATTATCACCATTCCCAAACACAACCTCTTTGATTTCTCTCTCTCCTCTCTCTCTCTCTCTAGAAGACTTCTTTCTTCTATATTGTGTAGTTAGTTAAGTAGAAGTAATAACTACATGGTAGGGTTAGGAAAGACGACCGAACGG
TARGET=1606,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3279
PRIMER_LEFT_PENALTY=0.093026
PRIMER_RIGHT_PENALTY=0.234845
PRIMER_LEFT_SEQUENCE=AAAAAGTGTCCCGAATCAGTGT
PRIMER_RIGHT_SEQUENCE=GGTCGTCTTTCCTAACCCTACC
PRIMER_LEFT=1435,22
PRIMER_RIGHT=1698,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.235
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.3279
PRIMER_LEFT_1_PENALTY=0.093026
PRIMER_RIGHT_1_PENALTY=0.234845
PRIMER_LEFT_1_SEQUENCE=AAAAGTGTCCCGAATCAGTGTT
PRIMER_RIGHT_1_SEQUENCE=GGTCGTCTTTCCTAACCCTACC
PRIMER_LEFT_1=1436,22
PRIMER_RIGHT_1=1698,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=60.235
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.3771
PRIMER_LEFT_2_PENALTY=0.142283
PRIMER_RIGHT_2_PENALTY=0.234845
PRIMER_LEFT_2_SEQUENCE=CCAAGTGTTTTACCCAATTCGT
PRIMER_RIGHT_2_SEQUENCE=GGTCGTCTTTCCTAACCCTACC
PRIMER_LEFT_2=1492,22
PRIMER_RIGHT_2=1698,22
PRIMER_LEFT_2_TM=60.142
PRIMER_RIGHT_2_TM=60.235
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.4893
PRIMER_LEFT_3_PENALTY=0.254467
PRIMER_RIGHT_3_PENALTY=0.234845
PRIMER_LEFT_3_SEQUENCE=TCCTTCACCAAGTGTTTTACCC
PRIMER_RIGHT_3_SEQUENCE=GGTCGTCTTTCCTAACCCTACC
PRIMER_LEFT_3=1485,22
PRIMER_RIGHT_3=1698,22
PRIMER_LEFT_3_TM=60.254
PRIMER_RIGHT_3_TM=60.235
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.4893
PRIMER_LEFT_4_PENALTY=0.254467
PRIMER_RIGHT_4_PENALTY=0.234845
PRIMER_LEFT_4_SEQUENCE=CATTCCCAAACACAACCTCTTT
PRIMER_RIGHT_4_SEQUENCE=GGTCGTCTTTCCTAACCCTACC
PRIMER_LEFT_4=1569,22
PRIMER_RIGHT_4=1698,22
PRIMER_LEFT_4_TM=60.254
PRIMER_RIGHT_4_TM=60.235
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_04388 Ps_contig_mira-and-tgicl-ass_franssen_33094
SEQUENCE=TTTTAAAGGAGCNTNNYTCATCAAACACAAATAGATATTTATATCATCATGTCTTTATTTTGTCCTAACATCATTATGCAACTCTCTTATCCTATATCATTCATTATATGCAACTCTTTTATCCTGTCTGTCTCTTTTAAATGCTTTTTTGTTTTGTTTTTTGTATTGAATTGTGTACTAAATATCGTCTTTTGAGTACATTTTCTCACCAACGGATACAGAGAATAACATCTAGTTTTATTTGATATAGAAGCTTCAAATAGCTTATTTCCATTAAGAGGGGAATTTTTGGGAGAGCAAAGCTTCAAGTGATGAATGATCAGTCACAGGGGCACTACAAGTGAAGTTTTGGCATACAAGAGCTACAACCTTGCCAACTGCACAGTTATTCTTGGCCATTAGAGCAATATTGCTGTTATTTACTTCCCAAAATTCCATATCTTCTTTGCTGTTGGGATCTATATGAATAACTGTTCTGTTGGGATCATATAATGTGTGAGCTGCGGCAAGCATGTTTTCAAATTCCTCGGAAGTCCTTTCGCCCACCACCACCACTTGCTTCCGGGAAGGCAAGCGCAGCATGTCTGTTGCACAACACATTAAAGGCACTGCCATGGCCATATCTTTCAATCTTTTCTCAAAAACTGCCAATAGATGTTCTGCATTTCGCTTATAGTAATCTGCCTTACTTCCAGAAACCATGGAAGCCAACCTTATAAGATTTATTGCAGAGACAGAGTTCCCCGACGGTTCTGCCCCGTCATGGTCTTCCTTCACACGGAGAAGAACTGAAGAATCTTCTCCTGTATTATTGAAATATCCGCCTCCATCCCTATCTAGAAACAAAGAATCCTGGGTTTCCTGTAATTCAATGGCCCATAAGAGCCAGTTGATTCCACCCCCGAATTCGTAGAGATCCAGCAATCCAGAAATCAAAAAAGCATAATCATCCAAGAAACCAGGAGCTTTAGATGGAGTGTTTCTGAAACTGTGTTGTAGTCTGCGAGTCTCTGCAATATAAAGTTGATTCCTAATGAAGGATGCTGCTTTATCTGCTATTCTCAAGTACTCCTTTGGTTCAGTGCCAACAACAGGAAAGTTGAATTTGATCCCTTCAGCTTCACCCTTTAGAATCTTAGAAGCCCTAGCAAAAGATGAGATTGCCAGTCCATTCCAAGATACAATAACCTTATCATCCAAATGTGGCTTTGGGCGCCTCAACCGTACTTCAAACAACTTACGCCTGCATTCTCCAAGAATTTCTTGATATGTCTCAACCGACAGACCATATTTTGATGCAATTTNTGAAGGATCCTTCCTCTCAATCAGGACATTCTTTCCCCCG
TARGET=543,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04389 Ps_contig_mira-and-tgicl-ass_franssen_33096
SEQUENCE=GTCGGGGTAAACTTAGTACTAACTATTCTTCCTATTCTTCATTTCTTCTTCAAAAACTCTTTACTTATTCTTAAAAAAACACTTTTTAGTGTCATAAAAAGACATCAACAACAACAAAGACATTGAACAAGAAACAACATGAAGTATGTTCTTGTAACTGGAGGAGTTGTTAGTGGACTTGGTAAAGGTGTTACAGCAAGTAGTATTGGTTTACTTCTCAAAGCTTGTGGACTTCGYGTCACTGCTATCAAAATCGATCCTTACTTGAACACAGATGCTGGAACAATGTCACCTATTGAACATGGAGAAGTTTATGTGTTGGATGATGGTGGTGAGGTGGACCTGGATCTTGGAAACTATGAGCGCTTTATGGATGTTAAGTTGACCCGTGATAATAACATAACTACAGGGAAGATTTACCAGTCTGTCATTGATAAGGAGAGAAGAGGAGACTATTTAGGAAAAACTGTACAGGTTGTCCCTCATATAACTGATGCTATTCAAGATTGGATAGAAAGAGTGGCCCACATACCAGTGGATGGAAAAGAAGGTCCAGCTGATGTATGTGTCATAGAATTGGGTGGAACCATAGGTGATATTGAGTCCATGCCTTTTATTCAAGCATTAGGACATTTTTCATATAGAGTAGGTGCTAGCAACTTTTGCTTGGTTTTCTTATTGNTTAGCCTTGTTCCTGTTTTGAATGTTGTTGGTGAACAAAAAACAAAACCAACACAACATAGTGTGAGAGGACTTAGAAGCCAAGGGCTGACTCCTCAAATCTTGGCATGCCGCAGCACAATGGCGCTAGATGAGAATGCAAAGACGAAGCTCTCTCAATTTTGTCTACTTCCGGGGGAAAATATAATTACTCTTTACGATGTTCCCAACATATGGCACATTCCTTTGCTTTTAAGAGATCAGAAGGCTCATGAAGCAATATTTAAAGTGCTGAACCTTACAGGTATCGCTCAAGAGCCTAATTTAGAGGAATGGACTTGTAGAGCTGAATCTTCTGATTTACTTCATGAACCAGTTCGTATAGCGTTAGTCGGGAAATATACATGCCTTTCAGATTCCTACCTCTCAGTTCTAAAGGCCTTAATTCATGCTTCTGTTAGTTGCCAAAAGAAACTTGTTGTGGACTGGATTTCAGCTACCAACCTCGAAAACGCGACTGCGAAAGAGAATCCTGATGTTTATAAGGCATCGTGGAAGTTATTAAAGGGTGCTGACGGTATTCTTGTTCCTGGAGGCTTTGGTGATAGAGGAGTGCAAGGAAAAATTATCGCAGCTAAATATGCTCGAGAAAACAGAATTCCATACCTCGGCATCTGTCTTGGAATGCAAGTTGCTGTCATAGAATTTGCACGATCCGTGCTTGGTTTAAAAGATGCTAATAGTACTGAAATTGATCCAAATACCAAAAGTCCATGCGTCATATTTATGCCTGAGGGATCAAAAACACACATGGGAGGCACCATGCGTCTTGGATCGAGGAGGACATATTTCCAAACTAAGTTATGCAAATCTGCAAAACTGTATGGCGGCAAAAGCTTCATTGATGAGAGACATCGGCATAGATATGAGGTGAACCCTGATTTGGTAACTAGCCTTGAAAATTCAGGTCTTTCCTTTACCGGAAAGGACGAAACTGGTCAACGCATGGAGATTGTTGAGATACCGAATCATCCTTATTTTGTCGGAGTCCAATTCCATCCTGAGTTCAAATCAAGACCAGGAAGGCCTTCTCCAGTGTTCTTAGGGTTTATCGCAGCCGCATGTGGACAACTTGAAGCTGTTTTACAACACTCTTCAAGGGGAGTAAGCAGTGATAATATTTCATCAGGCAAAACATATCAAAATGGAACAGCAACAAAAGCACAAGCTTTTAAGCCAGAATATATATATGGCAATGGAAATGCTAATGGAAATGGGTTTCATTACTAAATGGTTTTATAACAAGTACTAGTGTTTACTTGATGTGTTAGAAATTTTAAGTTTAATTGGTAAAATATGGTGTTGTTAAGAGAAGATGATTGATTGTACAGTAAAAAAAAATTGAAAGTTGGTAGGAATAGAGTTTTAAGGATGATGATGATGATGATGATACAGAGAGAGATGAAGCATTGACTTCCCGGTTTA
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04390 Ps_contig_mira-and-tgicl-ass_franssen_33096
SEQUENCE=GTCGGGGTAAACTTAGTACTAACTATTCTTCCTATTCTTCATTTCTTCTTCAAAAACTCTTTACTTATTCTTAAAAAAACACTTTTTAGTGTCATAAAAAGACATCAACAACAACAAAGACATTGAACAAGAAACAACATGAAGTATGTTCTTGTAACTGGAGGAGTTGTTAGTGGACTTGGTAAAGGTGTTACAGCAAGTAGTATTGGTTTACTTCTCAAAGCTTGTGGACTTCGYGTCACTGCTATCAAAATCGATCCTTACTTGAACACAGATGCTGGAACAATGTCACCTATTGAACATGGAGAAGTTTATGTGTTGGATGATGGTGGTGAGGTGGACCTGGATCTTGGAAACTATGAGCGCTTTATGGATGTTAAGTTGACCCGTGATAATAACATAACTACAGGGAAGATTTACCAGTCTGTCATTGATAAGGAGAGAAGAGGAGACTATTTAGGAAAAACTGTACAGGTTGTCCCTCATATAACTGATGCTATTCAAGATTGGATAGAAAGAGTGGCCCACATACCAGTGGATGGAAAAGAAGGTCCAGCTGATGTATGTGTCATAGAATTGGGTGGAACCATAGGTGATATTGAGTCCATGCCTTTTATTCAAGCATTAGGACATTTTTCATATAGAGTAGGTGCTAGCAACTTTTGCTTGGTTTTCTTATTGNTTAGCCTTGTTCCTGTTTTGAATGTTGTTGGTGAACAAAAAACAAAACCAACACAACATAGTGTGAGAGGACTTAGAAGCCAAGGGCTGACTCCTCAAATCTTGGCATGCCGCAGCACAATGGCGCTAGATGAGAATGCAAAGACGAAGCTCTCTCAATTTTGTCTACTTCCGGGGGAAAATATAATTACTCTTTACGATGTTCCCAACATATGGCACATTCCTTTGCTTTTAAGAGATCAGAAGGCTCATGAAGCAATATTTAAAGTGCTGAACCTTACAGGTATCGCTCAAGAGCCTAATTTAGAGGAATGGACTTGTAGAGCTGAATCTTCTGATTTACTTCATGAACCAGTTCGTATAGCGTTAGTCGGGAAATATACATGCCTTTCAGATTCCTACCTCTCAGTTCTAAAGGCCTTAATTCATGCTTCTGTTAGTTGCCAAAAGAAACTTGTTGTGGACTGGATTTCAGCTACCAACCTCGAAAACGCGACTGCGAAAGAGAATCCTGATGTTTATAAGGCATCGTGGAAGTTATTAAAGGGTGCTGACGGTATTCTTGTTCCTGGAGGCTTTGGTGATAGAGGAGTGCAAGGAAAAATTATCGCAGCTAAATATGCTCGAGAAAACAGAATTCCATACCTCGGCATCTGTCTTGGAATGCAAGTTGCTGTCATAGAATTTGCACGATCCGTGCTTGGTTTAAAAGATGCTAATAGTACTGAAATTGATCCAAATACCAAAAGTCCATGCGTCATATTTATGCCTGAGGGATCAAAAACACACATGGGAGGCACCATGCGTCTTGGATCGAGGAGGACATATTTCCAAACTAAGTTATGCAAATCTGCAAAACTGTATGGCGGCAAAAGCTTCATTGATGAGAGACATCGGCATAGATATGAGGTGAACCCTGATTTGGTAACTAGCCTTGAAAATTCAGGTCTTTCCTTTACCGGAAAGGACGAAACTGGTCAACGCATGGAGATTGTTGAGATACCGAATCATCCTTATTTTGTCGGAGTCCAATTCCATCCTGAGTTCAAATCAAGACCAGGAAGGCCTTCTCCAGTGTTCTTAGGGTTTATCGCAGCCGCATGTGGACAACTTGAAGCTGTTTTACAACACTCTTCAAGGGGAGTAAGCAGTGATAATATTTCATCAGGCAAAACATATCAAAATGGAACAGCAACAAAAGCACAAGCTTTTAAGCCAGAATATATATATGGCAATGGAAATGCTAATGGAAATGGGTTTCATTACTAAATGGTTTTATAACAAGTACTAGTGTTTACTTGATGTGTTAGAAATTTTAAGTTTAATTGGTAAAATATGGTGTTGTTAAGAGAAGATGATTGATTGTACAGTAAAAAAAAATTGAAAGTTGGTAGGAATAGAGTTTTAAGGATGATGATGATGATGATGATACAGAGAGAGATGAAGCATTGACTTCCCGGTTTA
TARGET=1902,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04391 Ps_contig_mira-and-tgicl-ass_franssen_33096
SEQUENCE=GTCGGGGTAAACTTAGTACTAACTATTCTTCCTATTCTTCATTTCTTCTTCAAAAACTCTTTACTTATTCTTAAAAAAACACTTTTTAGTGTCATAAAAAGACATCAACAACAACAAAGACATTGAACAAGAAACAACATGAAGTATGTTCTTGTAACTGGAGGAGTTGTTAGTGGACTTGGTAAAGGTGTTACAGCAAGTAGTATTGGTTTACTTCTCAAAGCTTGTGGACTTCGYGTCACTGCTATCAAAATCGATCCTTACTTGAACACAGATGCTGGAACAATGTCACCTATTGAACATGGAGAAGTTTATGTGTTGGATGATGGTGGTGAGGTGGACCTGGATCTTGGAAACTATGAGCGCTTTATGGATGTTAAGTTGACCCGTGATAATAACATAACTACAGGGAAGATTTACCAGTCTGTCATTGATAAGGAGAGAAGAGGAGACTATTTAGGAAAAACTGTACAGGTTGTCCCTCATATAACTGATGCTATTCAAGATTGGATAGAAAGAGTGGCCCACATACCAGTGGATGGAAAAGAAGGTCCAGCTGATGTATGTGTCATAGAATTGGGTGGAACCATAGGTGATATTGAGTCCATGCCTTTTATTCAAGCATTAGGACATTTTTCATATAGAGTAGGTGCTAGCAACTTTTGCTTGGTTTTCTTATTGNTTAGCCTTGTTCCTGTTTTGAATGTTGTTGGTGAACAAAAAACAAAACCAACACAACATAGTGTGAGAGGACTTAGAAGCCAAGGGCTGACTCCTCAAATCTTGGCATGCCGCAGCACAATGGCGCTAGATGAGAATGCAAAGACGAAGCTCTCTCAATTTTGTCTACTTCCGGGGGAAAATATAATTACTCTTTACGATGTTCCCAACATATGGCACATTCCTTTGCTTTTAAGAGATCAGAAGGCTCATGAAGCAATATTTAAAGTGCTGAACCTTACAGGTATCGCTCAAGAGCCTAATTTAGAGGAATGGACTTGTAGAGCTGAATCTTCTGATTTACTTCATGAACCAGTTCGTATAGCGTTAGTCGGGAAATATACATGCCTTTCAGATTCCTACCTCTCAGTTCTAAAGGCCTTAATTCATGCTTCTGTTAGTTGCCAAAAGAAACTTGTTGTGGACTGGATTTCAGCTACCAACCTCGAAAACGCGACTGCGAAAGAGAATCCTGATGTTTATAAGGCATCGTGGAAGTTATTAAAGGGTGCTGACGGTATTCTTGTTCCTGGAGGCTTTGGTGATAGAGGAGTGCAAGGAAAAATTATCGCAGCTAAATATGCTCGAGAAAACAGAATTCCATACCTCGGCATCTGTCTTGGAATGCAAGTTGCTGTCATAGAATTTGCACGATCCGTGCTTGGTTTAAAAGATGCTAATAGTACTGAAATTGATCCAAATACCAAAAGTCCATGCGTCATATTTATGCCTGAGGGATCAAAAACACACATGGGAGGCACCATGCGTCTTGGATCGAGGAGGACATATTTCCAAACTAAGTTATGCAAATCTGCAAAACTGTATGGCGGCAAAAGCTTCATTGATGAGAGACATCGGCATAGATATGAGGTGAACCCTGATTTGGTAACTAGCCTTGAAAATTCAGGTCTTTCCTTTACCGGAAAGGACGAAACTGGTCAACGCATGGAGATTGTTGAGATACCGAATCATCCTTATTTTGTCGGAGTCCAATTCCATCCTGAGTTCAAATCAAGACCAGGAAGGCCTTCTCCAGTGTTCTTAGGGTTTATCGCAGCCGCATGTGGACAACTTGAAGCTGTTTTACAACACTCTTCAAGGGGAGTAAGCAGTGATAATATTTCATCAGGCAAAACATATCAAAATGGAACAGCAACAAAAGCACAAGCTTTTAAGCCAGAATATATATATGGCAATGGAAATGCTAATGGAAATGGGTTTCATTACTAAATGGTTTTATAACAAGTACTAGTGTTTACTTGATGTGTTAGAAATTTTAAGTTTAATTGGTAAAATATGGTGTTGTTAAGAGAAGATGATTGATTGTACAGTAAAAAAAAATTGAAAGTTGGTAGGAATAGAGTTTTAAGGATGATGATGATGATGATGATACAGAGAGAGATGAAGCATTGACTTCCCGGTTTA
TARGET=2091,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04392 Ps_contig_mira-and-tgicl-ass_franssen_33100
SEQUENCE=AATGGCAATAAATAAACAACATTAGTCTATAACAGGRGTTTTACTGCATTCCACACAGCAAAACACACTAACCCTTAAACCCTAACTCTCTCCGTCGCAATGAAATCCGTCGGCGTTCTAATGGCCATCCCAATGCCGAGCAACCTCGCCGAAAAACTCGCCAACCGTTTCAACCTCTTCAAACTCTGGACCTATTCTTCCATCGAAGCTTTTTCCGAAACGCACGCCGATTCAGTTCGTGCATTCGTTTGCGACGCCAAATCCTGTGCCGACGCAAAAACTATCGACTCTCTTCCGAATCTGGAGATTGTTTCCACTAACAGCGTTGGATTTGATAAAATTGATATCGGGAAATGCAGAGAGAGAGGAATTCGAGTTACTAATACGCCTGATGTGTTGACGGATGATGTTGCTGATCACGCTATTGGTCTTGCTTTGGCTGTGTTCAGGAAGATTCCGAATAGTGATGGCTTTGTCAAGAATGGGCTTTGGAAACATTCTCATTATCCATTAACTACAAAGTTTAGTGGTAAAGAAGTTGGAGTTGTTGGTTTGGGAAGAATAGGTTCAGCAATAGCAAAGAGGGCTGCAGCATTTGGGTGTCCAGTTAGTTACCACTCAAGATCTCAAAAACCAGAGGCAGGATCATACAAGTATTACCCTAACATCCTTGATTTGGCAGCTAACTCTCAAGTACTTGTTGTCGCATGTGCTCTAACCGAACAAACGCGAACAATAATTGTAGAACCGTGAAGTTATTGATGCATTCGG
TARGET=358,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04393 Ps_contig_mira-and-tgicl-ass_franssen_33105
SEQUENCE=ACTAGGAAATAACAAGTTAACACGAAATACATGGGAAGTTACATGAATCATTGAGCAAGGAAATTGTAGTAGGGTACCGGACTTTGTATTTCTGGCTCATATAGTGGATGTCACATCCTCTATGCATGTTCAATTCGGTCTGAGATCATTTGCTTCTTTACCGTTCACAACAAGGTTCTTCTTGATCCCGTTATTTCCAATCCCAGTGATATCTTTGTTAGCGATGTGGCTTTGGTCCAAGACGTTTCTTCTTTCTTTCTACTATCTTAAGGATAGACTACATCAAACATGGGTTGTCCCTAGGTGTGGCTTTCAGTATTTCTTACCATTTGCGACTGAGGGTATCAATAATCATATTGAGAAAGCTATCCTCACGGCAGATAAAATTGGTGTTAAAGTCATAAGCTTGGCTGCTTTGAATAAGAATGAATCTCTTAACGGTGGAGGAAAACTCTTCGTGGACAAGTACCCAAATCTCAGAGTTCGTGTCGTTCATGGCAACACTTTAACAGCAGCTGTGATCTTAGATGAAATCCCCAAAGATGCTAAAGAGGTTTTCCTTACTGGTGCAACTTCCAAGCTTGGAAGAGCAATTGCTCTCTATCTCTGCAGAAACAAAGTCAAAGTTCTGATGTTAACACTATCAACAGATAGGTTTAAGAAGATTCAAAAGGAAGCACCCCAACAATACCAAAGTTACCTAGTTCAAGTGACAAAATACCAAGCAGCACAGCACTGCAAGACGTGGATAGTTGGCAAGTGGATCACTCCCAGAGAGCAAAACTGGGCGCCAAGAGGAACACATTTCCACCAATTTGTTGTGCCTCCGATATTTGGATTTAGAAGAGATTGCACCTATGGTGATCTAGCAGCAATGAGATTGCCGGAGGATGTGGAAGGTCTTGGATGCTGTGAGTACACGATGGATAGAGNAGTTGTTCATGCATGCCATGCAGGAGGAGTTGTGCATAGTCTTGAGGGTTGGACTCATCATGAAGTTGGAGCCATTGATGTTGATAGAATCGATGTTGTGTGGAAAGCAGCACTCAAACATGGTTTAACGCCTGTGTCTTCGGGATCAACTGCAAAACCAAATATAATTTGAATTAAATTTGGTCAAAATACTAAATATATCCAAACATAAATACAAAGGAGACATGCTGTAAGGAGAGTAAAGACCTATGCACTGGTCCTTAAGATGATTAATTTCTGTCTGTCTGTCTGCATTTAATAATCACTTYCATGTCAGCTGTTATAGTGACTCATTTTCATGTGCTTAGATTCACTTCACCATATGCTTTCTCATTCTTGTTGAATCTTCCCATCCCTTCTTGGAAATGAATATGTCTATATTTTAAATTARRNNATTTTAAACTTTTAGTTCCCTTAAAAAAATTAGCTTTTTATCCTTACAAAAATT
TARGET=249,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04394 Ps_contig_mira-and-tgicl-ass_franssen_33105
SEQUENCE=ACTAGGAAATAACAAGTTAACACGAAATACATGGGAAGTTACATGAATCATTGAGCAAGGAAATTGTAGTAGGGTACCGGACTTTGTATTTCTGGCTCATATAGTGGATGTCACATCCTCTATGCATGTTCAATTCGGTCTGAGATCATTTGCTTCTTTACCGTTCACAACAAGGTTCTTCTTGATCCCGTTATTTCCAATCCCAGTGATATCTTTGTTAGCGATGTGGCTTTGGTCCAAGACGTTTCTTCTTTCTTTCTACTATCTTAAGGATAGACTACATCAAACATGGGTTGTCCCTAGGTGTGGCTTTCAGTATTTCTTACCATTTGCGACTGAGGGTATCAATAATCATATTGAGAAAGCTATCCTCACGGCAGATAAAATTGGTGTTAAAGTCATAAGCTTGGCTGCTTTGAATAAGAATGAATCTCTTAACGGTGGAGGAAAACTCTTCGTGGACAAGTACCCAAATCTCAGAGTTCGTGTCGTTCATGGCAACACTTTAACAGCAGCTGTGATCTTAGATGAAATCCCCAAAGATGCTAAAGAGGTTTTCCTTACTGGTGCAACTTCCAAGCTTGGAAGAGCAATTGCTCTCTATCTCTGCAGAAACAAAGTCAAAGTTCTGATGTTAACACTATCAACAGATAGGTTTAAGAAGATTCAAAAGGAAGCACCCCAACAATACCAAAGTTACCTAGTTCAAGTGACAAAATACCAAGCAGCACAGCACTGCAAGACGTGGATAGTTGGCAAGTGGATCACTCCCAGAGAGCAAAACTGGGCGCCAAGAGGAACACATTTCCACCAATTTGTTGTGCCTCCGATATTTGGATTTAGAAGAGATTGCACCTATGGTGATCTAGCAGCAATGAGATTGCCGGAGGATGTGGAAGGTCTTGGATGCTGTGAGTACACGATGGATAGAGNAGTTGTTCATGCATGCCATGCAGGAGGAGTTGTGCATAGTCTTGAGGGTTGGACTCATCATGAAGTTGGAGCCATTGATGTTGATAGAATCGATGTTGTGTGGAAAGCAGCACTCAAACATGGTTTAACGCCTGTGTCTTCGGGATCAACTGCAAAACCAAATATAATTTGAATTAAATTTGGTCAAAATACTAAATATATCCAAACATAAATACAAAGGAGACATGCTGTAAGGAGAGTAAAGACCTATGCACTGGTCCTTAAGATGATTAATTTCTGTCTGTCTGTCTGCATTTAATAATCACTTYCATGTCAGCTGTTATAGTGACTCATTTTCATGTGCTTAGATTCACTTCACCATATGCTTTCTCATTCTTGTTGAATCTTCCCATCCCTTCTTGGAAATGAATATGTCTATATTTTAAATTARRNNATTTTAAACTTTTAGTTCCCTTAAAAAAATTAGCTTTTTATCCTTACAAAAATT
TARGET=1209,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04395 Ps_contig_mira-and-tgicl-ass_franssen_33107
SEQUENCE=ATAATAATAATAATAATAATGACAAGCAGATGAGATTCTAAATCTAATCACAACCCCAATCGACTTCCGTTTGAAGAGTCGCGCTTCAATCTTGTTCGGAGTCGCGCTGAACTCCATTGACCGGAAAACGCAGCCGGATTTCGCCGTCGTAAGGAGGAAGGCCACCACAGACAGTTAAGTATTTGATTGGAAATTCTATATTCTTTATAGGAAAGTTTTAGAGTATAAGAAGAAGCTTCAATGGCCAAGAAACCTGCACCAAGATCCAGTAAATTATCTGTCTACCTGTACGTTCCTAATATCATTGGATACATCCGGGTGCTTTTGAATTGTCTTGCCTTCTCTTTATGTTTAACAAACAAAATTGTTTTCCCTATTCTCTACTTTTTCAGTTTTGTATGTGATGCTGTGGATGGCTGGTGTGCTCGAAGATTCAATCAAGTATCAACATTTGGAGCTGTGCTGGATATGGTAACAGACAGGATTAGTACTGCTTGTCTTCTTGTAGTTCTATCCCAATTATACAAGCCTGGCCTGATCTTCTTGTCATTGCTCGCCTTAGACATTGGCAGCCACTGGTTTCAAATGTACAGCACTTTCTTGGCTGGCAAGGCTAGCCATAAAGATGTAAAAGACAGCACCAACTGGCTTTTCAGGGCATATTATGGAAATAGGATGTTTATGGCTTACTGCTGTGTCTCATGCGAGGTTCTTTACCTGATCATGTTTTATCTTGCGGAGAATGAAACAGAGACATTGGTGGATGTTTTATCAAGTAATTTACAAAAGATAACCCTAATCTCTTTCCTAATTGCCACAAGTTTATTTGGATGGGCAACCAAGCAAATTATAAATGTTATTCAGATGAAGACAGCCGCAGACATGTGTGTGCTTTATGATATACAAACAAAAAACAAGAAGTACTGATTGTTTACTCTATGTCCACGACGTCCCTTCGTTCCTTCGTCCCTTTTCGTCCCTTTACG
TARGET=1,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04396 Ps_contig_mira-and-tgicl-ass_franssen_33115
SEQUENCE=GTTGGNNAAGAACTCCAATGAATTACTAGTATTAGGGTTTATCAAAAAGAGAGAAAGAAAGAGAAAATGGGAGGCAAGGGAAGGAAAAGGAGAGAGAAGAACTACAAGGCTGCTCACGGTGGCGCCACCGCCCTCCCTCCACCGCCTAAATCCTCTCAACTCGATGCTCTTCCCTTCAAACTTCGCCAAATCATCTCTTTCTCTAAAAACCAAAACGATTCTTCTGGCTTATCAAAGAAATTAGATGGTGGAGAGGCTCAAAAGGTGGATACTAAGGTGGATACTAGTGCACCATTGAAGGCACGTCAACCCAGTGATGAACAGCCTAGTGGAAATGATGATAAGAATAAGAAAAAAAGAAAGAGGAAGGAGGTTAAAGATCTCCGGTTTGCAATGGGTGAGGATAAAACGAATGCACAGTTAAAGAAAAAGGAACGCAAAAAGAAGTATGAAGCGAAAAAGAAGAAGCACAAAAAGGTTGAAGAAGATGAATTTTTGGACTTTCCTGGAAAAGAAAAAATAAAATTTGGAGATATTGTACAAGCCCCACCGAAGTTATCTTTCATTCCAAAAGGAGTCAAGATTTCACAGGATGCTTCTCATGAGAGACTTAGACTCCGAGCTATTGAAGAATACAGGAGTCGCAAAGCATGGACATCAAGACCAGGAAATCACCGCCCACCTCCGGTGACTACATCGGACCCTTAAATTGTATGCACCATGTTCTTAAGGATACTCTGCTTAACCAAGGGAGTTTTCTGAACAAATAAGAGAAATTCAACACGGCTCTTTGATACTGTCCTCCTGGAAGGTGGGTTTTTCTTCATTCAGAAGTAACTTACATGTTCTTTATAGAAAGTATGACCAAATGATATACTTGCACTTGTGCTGAAGTCATTAGTTACATTCAGTTTGATCCATGAAAAGTTGACGTCTGAGTTATTAGATTGGTTAAAGTTTTGTTAAAATAAGAGAAAAATATCGACTCAAAATGACGCCGCTGTTTGTATTTGTGTCTCTAAGTATTCTTTAATTTATGTTTTCCTTGCTCTCAAATTTGTATTTGGAAATATATAGACTATTGAGATATAAAGTAAAGTAAAGATTATGGCATGAGTCAAAACYTAATTGACTAGCAGAACTTCACTGGATGAGTGCCTGATCCTTTGAAAC
TARGET=1090,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04397 Ps_contig_mira-and-tgicl-ass_franssen_33130
SEQUENCE=ATACCAAAAGAAAAAAAATTAAAACCCTAAAACAACAACAACAACTCAGCAGCTCCCATAAGCTTCGGCACCTTCTATCATAAACACGCTGTAGTCCTCTCCTCGCCGGAAAAGCAACCGGAGTTGGTCGTCGGAAACCAGAAAAGTTTTTAGTGAAATTACCGATTCATCTTCATGGCTAATAATCCATCACAGCTTCTTCCATCAGAGTTGATTGACCGCTGTATAGGTTCAAAAATATGGGTGATAATGAAAGGTGACAAGGAGCTTGTTGGTACTCTTAGAGGTTTTGATGTGTATGTCAACATGGTCCTTGAAGATGTTACTGAATATGAGATCACTGCCGAGGGGAGAAGGATAACTAAGCTTGATCAGATTTTACTCAATGGAAACAACATTGCCATTTTGGTTCCTGGTGGTTCACCTGATCCAGAATGAATAATGCTACTTGGTTACATCTATTTTTAACTCTTAGTTGTTGCACAAGGTGCATAATTCCTATATTTTAAAGATGATTCTGAGACAAGCTAGGTTTTTTTTCTTCCGTGAGCTTGTTAAGGATACATGATCGAAGTAACTGTTAAACCTTTATCATTATGTTGAATTGTGGANTTTTCTTAGTGTAACAGYTTNTTTTACCGTTTAAAGGTTTACGGTTAAAAAACCCGTAAAAGGTACCTTTAAAG
TARGET=31,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04398 Ps_contig_mira-and-tgicl-ass_franssen_33132
SEQUENCE=ATGATAGATTAAACTTTAATGTATGAGAAAAACATCATCATACAAAAAAGAACACACGACAATGGTCTACAATGTTTTTCCCTAGTTAGATAACCTTTTCTTCCATCATCCACTATACAAGAGAAACGAAAAATGTAAGTATATTATTTAACGCAAATCAACAACACTCAGAAACTGAGACTGTGAAGAACCTTAGTAGTTAGTTAGTAGACATGAGAATGAAGCTGCTGATTTTGTTTGCATGCTTAGTTTCTGGGTTTATCTCCTCTAGATCTGAGACACTGTTTTCTTTCTCACAAAACGATGTCGTCTCACCTAATCCATTGCAACAACTTATGGTTAACCTCACTCTCATTCAAGGAGCTGATTCCAAAGGAGCTGTCTGTTTGGATGGGACATTGCCTGGTTACCATTTGGATCGTGGATTTGAATCTGGTGCAAATAGTTGGCTTATTCATCTTGAGGGAGGAGGATGGTGTAACACTATCAGAAACTGTGTGTATAGAAAAAAGACTCGTCGTGGTTCCTCAAACTACATGGAAAATCAAATACCATTCACAGGAATATTGAGTAATAAACCAGAAGAAAACCCTGATTTCTTTAACTGGAATAGAGTCAAATTAAGGTACTGTGATGGAGCATCTTTTACAGGAGATAGTGAAAATGAGGCTGCAGGACTTCAGTTTCGAGGACAAAAAATATGGCTAGCTGCAATAGATGAATTAATGTCTCAAGGAATGCATAATGCCGAACAGGCCCTTCTCTCTGGATGTTCTGCGGGTGGCCTTGCATCCATACTACATTGTGATGAGTTCCAGAGCTTGTTACCAAAATCTTCCAAAGTGAAGTGTTTTAGTGATGCAGGATTTTTTCTAGATGCAATTGATGTATCTGGAGGACGCACATTGAGAAATCTGTTCGGAGGCGTAGTTCAGTTACAGGAAGTACAAAAAAATCTGCCAAAAAATTGTCTCAACAAACTGGACCCAACTTCGTGCTTCTTTCCTCAGAATTTGGTTGAACATGTTGAGACACCGTTGTTTCTACTCAACACGGCTTATGATGTGTGGCAGGTTCAAGCCAGTTTAGCCCCAGTGACAGCCGATCCTCTTGGCTCTTGGAATGACTGCAAATCAAACCATGCAAATTGTAGCTCGTCTCAAATTCAGTTCCTCCAAGACTTCAGAAATCAAATGGTTGACGACCTGAAAGACTTCTCAAGACCGTCTCAAACTGGACTTTTCATAAATTCCTGTTTTGCTCATTGCCAGTCGGAGAGACAGGAGACATGGTTCGCGGATGACTCTCCTCTTCTCGAGGACAAGCCAATTGCAGTTGCTGTTGGAGACTGGTATTTCGACAGAGAAGTTGTAAAATCTATAGACTGTCCTTACCCTTGTGACAATAACTGCCATAATTTGGTGTTTAGAAGAGTATAATATGCTATCTTTTCATATGTTAAGATCACTCGTGTATTTAACATCCATTAATTATGTCAAGAAGATTCATATTCAYTAATTGCCATTTATTTTACCTTTGCATAGAATATCCAAAAACGAAAAATTAGAAATTTTGCTTGGGGCATTGAATAAAA
TARGET=197,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04399 Ps_contig_mira-and-tgicl-ass_franssen_33136
SEQUENCE=AGGTAGAGGAAGAGGAGGAAGAGGAGGAAGAGGAGGAGGAGGAGGAGGAAGTGGAAGAAGACACTGTGCCAACCCAAAACATTGATGATGATGAGAAGAAGAAACATGCTGAACTTCTTTCTCTTCCTCCTCACAAGTCTGAAGTTTACATAGGTGGCATTCCTCTTGATGCTGTAACGGAAGATTTAAAAGATTTTTGTGAGCGTATTGGGGAAGTTGTACAGGTTCGAATAATGAAAGGAAAAGATTCTTCGGAGAATAAGGGTTTTGCTTTTGTGCTTTTTAAGAATGTAGAACTGGCTTCTAGAGCCATTGAGGAGCTGAATAATACTGAATTTAAGGGTAAAAAAATAAGATGTTCTACATCTCAAGCAAAAAATCGTCTTTTTATTGGCAATATTCCTAGAAGCTGGGGCGATAACGATCTAAAAAAGGTTGTGAGTGAGATAGGACCTGGTGTTACCGCTGTTGAGCTAGTCAAGGATATGAAGAACATTATCAATAACCGCGGTTTTGCTTTTATTGACTATTATAATAATGCATGTGCTGAATATTCAAGGCAAAAGATGATGAACCCAACATTTAAGCTTGGTGACAACTCCCCAACAGTGAGCTGGGCTGAACCCGAAAAATGCTGATTCCTCTGCTTCATCTC
TARGET=30,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2744
PRIMER_LEFT_PENALTY=0.043085
PRIMER_RIGHT_PENALTY=0.231323
PRIMER_LEFT_SEQUENCE=GAAGAGGAGGAAGAGGAGGAAG
PRIMER_RIGHT_SEQUENCE=CGTTACAGCATCAAGAGGAATG
PRIMER_LEFT=8,22
PRIMER_RIGHT=178,22
PRIMER_LEFT_TM=59.957
PRIMER_RIGHT_TM=59.769
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=0.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=0.3831
PRIMER_LEFT_1_PENALTY=0.043085
PRIMER_RIGHT_1_PENALTY=0.340007
PRIMER_LEFT_1_SEQUENCE=GAAGAGGAGGAAGAGGAGGAAG
PRIMER_RIGHT_1_SEQUENCE=ATCAAGAGGAATGCCACCTATG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=169,22
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=60.340
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=0.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.4066
PRIMER_LEFT_2_PENALTY=0.043085
PRIMER_RIGHT_2_PENALTY=0.363508
PRIMER_LEFT_2_SEQUENCE=GAAGAGGAGGAAGAGGAGGAAG
PRIMER_RIGHT_2_SEQUENCE=CCCAATACGCTCACAAAAATCT
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=59.957
PRIMER_RIGHT_2_TM=60.364
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=0.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=204
PRIMER_PAIR_PENALTY_3=0.5016
PRIMER_LEFT_3_PENALTY=0.399914
PRIMER_RIGHT_3_PENALTY=0.101680
PRIMER_LEFT_3_SEQUENCE=AGAGGAAGAGGAGGAAGAGGAG
PRIMER_RIGHT_3_SEQUENCE=GTACAACTTCCCCAATACGCTC
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=221,22
PRIMER_LEFT_3_TM=59.600
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=0.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.5263
PRIMER_LEFT_4_PENALTY=0.043085
PRIMER_RIGHT_4_PENALTY=0.483253
PRIMER_LEFT_4_SEQUENCE=GAAGAGGAGGAAGAGGAGGAAG
PRIMER_RIGHT_4_SEQUENCE=TCTTCCGTTACAGCATCAAGAG
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=183,22
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=59.517
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=0.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=176
=
PRIMER_SEQUENCE_ID=SSR_04400 Ps_contig_mira-and-tgicl-ass_franssen_33136
SEQUENCE=AGGTAGAGGAAGAGGAGGAAGAGGAGGAAGAGGAGGAGGAGGAGGAGGAAGTGGAAGAAGACACTGTGCCAACCCAAAACATTGATGATGATGAGAAGAAGAAACATGCTGAACTTCTTTCTCTTCCTCCTCACAAGTCTGAAGTTTACATAGGTGGCATTCCTCTTGATGCTGTAACGGAAGATTTAAAAGATTTTTGTGAGCGTATTGGGGAAGTTGTACAGGTTCGAATAATGAAAGGAAAAGATTCTTCGGAGAATAAGGGTTTTGCTTTTGTGCTTTTTAAGAATGTAGAACTGGCTTCTAGAGCCATTGAGGAGCTGAATAATACTGAATTTAAGGGTAAAAAAATAAGATGTTCTACATCTCAAGCAAAAAATCGTCTTTTTATTGGCAATATTCCTAGAAGCTGGGGCGATAACGATCTAAAAAAGGTTGTGAGTGAGATAGGACCTGGTGTTACCGCTGTTGAGCTAGTCAAGGATATGAAGAACATTATCAATAACCGCGGTTTTGCTTTTATTGACTATTATAATAATGCATGTGCTGAATATTCAAGGCAAAAGATGATGAACCCAACATTTAAGCTTGGTGACAACTCCCCAACAGTGAGCTGGGCTGAACCCGAAAAATGCTGATTCCTCTGCTTCATCTC
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2456
PRIMER_LEFT_PENALTY=0.143960
PRIMER_RIGHT_PENALTY=0.101680
PRIMER_LEFT_SEQUENCE=GAGGAGGAAGTGGAAGAAGACA
PRIMER_RIGHT_SEQUENCE=GTACAACTTCCCCAATACGCTC
PRIMER_LEFT=41,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=59.856
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.2744
PRIMER_LEFT_1_PENALTY=0.043085
PRIMER_RIGHT_1_PENALTY=0.231323
PRIMER_LEFT_1_SEQUENCE=AAGAGGAGGAAGAGGAGGAAGA
PRIMER_RIGHT_1_SEQUENCE=CGTTACAGCATCAAGAGGAATG
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=178,22
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=59.769
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=0.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.2744
PRIMER_LEFT_2_PENALTY=0.043085
PRIMER_RIGHT_2_PENALTY=0.231323
PRIMER_LEFT_2_SEQUENCE=GAAGAGGAGGAAGAGGAGGAAG
PRIMER_RIGHT_2_SEQUENCE=CGTTACAGCATCAAGAGGAATG
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=178,22
PRIMER_LEFT_2_TM=59.957
PRIMER_RIGHT_2_TM=59.769
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=0.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.3753
PRIMER_LEFT_3_PENALTY=0.143960
PRIMER_RIGHT_3_PENALTY=0.231323
PRIMER_LEFT_3_SEQUENCE=GAGGAGGAAGTGGAAGAAGACA
PRIMER_RIGHT_3_SEQUENCE=CGTTACAGCATCAAGAGGAATG
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=178,22
PRIMER_LEFT_3_TM=59.856
PRIMER_RIGHT_3_TM=59.769
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=0.3831
PRIMER_LEFT_4_PENALTY=0.043085
PRIMER_RIGHT_4_PENALTY=0.340007
PRIMER_LEFT_4_SEQUENCE=AAGAGGAGGAAGAGGAGGAAGA
PRIMER_RIGHT_4_SEQUENCE=ATCAAGAGGAATGCCACCTATG
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=169,22
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=60.340
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=0.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_04401 Ps_contig_mira-and-tgicl-ass_franssen_33142
SEQUENCE=GGATCTTGAAAAAATCAATTGCGGCCGCCATGATTTCAATAATACCATTCATTTTAATTCACACTTTGTTTTATGTTGGATATTCTGATGATCTACCACTTACCACTACACTTCCACAATCACCAACATTATTCCTCCCTCCTTTTCACATATACCATCAAATATCCAAACCATATTCTTCATATGAACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCAAGGAGAAGATCTCCACCACCACCACCTAGGAGAAGATCCCCCCCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCTAGGATAAGATCTCCACCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCACCTAGGATAAGATCTCCACCACCTCCACCTCCATCACCACCACCACCTCCTCCATCACCACCACCACCACCACCACCTTCTCCATCACCACCACCACCTCTTTCATTTGACCATTTCGTGCTATCTCAGACATGGCCACCAACATATTGCAAGATTAAAAATAACAAATGTGTCTCTCCAGAGCCACTAAAGTTTGTCATTCACGGGTTGTGGCCAAGTAAAAAAGACACTCTGATACAAGATTGCGATGAAAAAATTATAGATGTTAATGACCTTGAGCCACTAATGGAAGAACTTAATGAAGATTGGCCAGCATTATTTGAAAATGATAACCAAACAGAAGCGAACATTCAACTATGGGTTGACCAGTGGAATGGTCACGGGACTTGTTCAGTTGAGTTGTTCGAATTTGTTCCTTACTTTGAAGAGACATTAAATGTTTACCATCGAAATAGTATAAAAGACATCCTTATAGAGAATGATATTAATCCGGGCGGAACGTTTCCTAAAGAAATTATTCTCAATGCAATACACACACATATTTTATTTATGCCACAACTTCGATGCAAGCGGATTAATGATACAGATTATTTATTTGAAATAAGACTTTGCTTGACAGCAAGTGTTGAACTAGAGTATAAAGATTGTGATACTCCTTATAGTGGTTGTCACAATGAGGATGTGTATTTTTAATAAATTATAATGTTATTTTATGGTCATGATGTCTGAGCTTTTGAAGTTCAAAGGATGCTACTAAAGCTTCATAGGAGTGTGAAGTTTGTCTCTTATTTTAGTTTTCTACTATGTAATTATTTTTCTTTTTTCCCGTGTTTTATTTGTTGGATTGTTGTTGTTTAATTTTAGTTACTAAACNCCTTC
TARGET=237,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1707
PRIMER_LEFT_PENALTY=0.061184
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_SEQUENCE=ATCTTATTCTTGGTGGTGGTGG
PRIMER_LEFT=128,22
PRIMER_RIGHT=290,22
PRIMER_LEFT_TM=59.939
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.1707
PRIMER_LEFT_1_PENALTY=0.061184
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_1_SEQUENCE=ATCTTATTCTTGGTGGTGGTGG
PRIMER_LEFT_1=128,22
PRIMER_RIGHT_1=350,22
PRIMER_LEFT_1_TM=59.939
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.1786
PRIMER_LEFT_2_PENALTY=0.061184
PRIMER_RIGHT_2_PENALTY=0.117452
PRIMER_LEFT_2_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_2_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT_2=128,22
PRIMER_RIGHT_2=305,22
PRIMER_LEFT_2_TM=59.939
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=0.1786
PRIMER_LEFT_3_PENALTY=0.061184
PRIMER_RIGHT_3_PENALTY=0.117452
PRIMER_LEFT_3_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_3_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT_3=128,22
PRIMER_RIGHT_3=335,22
PRIMER_LEFT_3_TM=59.939
PRIMER_RIGHT_3_TM=60.117
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.1786
PRIMER_LEFT_4_PENALTY=0.061184
PRIMER_RIGHT_4_PENALTY=0.117452
PRIMER_LEFT_4_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_4_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT_4=128,22
PRIMER_RIGHT_4=365,22
PRIMER_LEFT_4_TM=59.939
PRIMER_RIGHT_4_TM=60.117
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_04402 Ps_contig_mira-and-tgicl-ass_franssen_33142
SEQUENCE=GGATCTTGAAAAAATCAATTGCGGCCGCCATGATTTCAATAATACCATTCATTTTAATTCACACTTTGTTTTATGTTGGATATTCTGATGATCTACCACTTACCACTACACTTCCACAATCACCAACATTATTCCTCCCTCCTTTTCACATATACCATCAAATATCCAAACCATATTCTTCATATGAACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCAAGGAGAAGATCTCCACCACCACCACCTAGGAGAAGATCCCCCCCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCTAGGATAAGATCTCCACCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCACCTAGGATAAGATCTCCACCACCTCCACCTCCATCACCACCACCACCTCCTCCATCACCACCACCACCACCACCACCTTCTCCATCACCACCACCACCTCTTTCATTTGACCATTTCGTGCTATCTCAGACATGGCCACCAACATATTGCAAGATTAAAAATAACAAATGTGTCTCTCCAGAGCCACTAAAGTTTGTCATTCACGGGTTGTGGCCAAGTAAAAAAGACACTCTGATACAAGATTGCGATGAAAAAATTATAGATGTTAATGACCTTGAGCCACTAATGGAAGAACTTAATGAAGATTGGCCAGCATTATTTGAAAATGATAACCAAACAGAAGCGAACATTCAACTATGGGTTGACCAGTGGAATGGTCACGGGACTTGTTCAGTTGAGTTGTTCGAATTTGTTCCTTACTTTGAAGAGACATTAAATGTTTACCATCGAAATAGTATAAAAGACATCCTTATAGAGAATGATATTAATCCGGGCGGAACGTTTCCTAAAGAAATTATTCTCAATGCAATACACACACATATTTTATTTATGCCACAACTTCGATGCAAGCGGATTAATGATACAGATTATTTATTTGAAATAAGACTTTGCTTGACAGCAAGTGTTGAACTAGAGTATAAAGATTGTGATACTCCTTATAGTGGTTGTCACAATGAGGATGTGTATTTTTAATAAATTATAATGTTATTTTATGGTCATGATGTCTGAGCTTTTGAAGTTCAAAGGATGCTACTAAAGCTTCATAGGAGTGTGAAGTTTGTCTCTTATTTTAGTTTTCTACTATGTAATTATTTTTCTTTTTTCCCGTGTTTTATTTGTTGGATTGTTGTTGTTTAATTTTAGTTACTAAACNCCTTC
TARGET=267,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1707
PRIMER_LEFT_PENALTY=0.061184
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_SEQUENCE=ATCTTATTCTTGGTGGTGGTGG
PRIMER_LEFT=128,22
PRIMER_RIGHT=350,22
PRIMER_LEFT_TM=59.939
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=223
PRIMER_PAIR_PENALTY_1=0.1786
PRIMER_LEFT_1_PENALTY=0.061184
PRIMER_RIGHT_1_PENALTY=0.117452
PRIMER_LEFT_1_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_1_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT_1=128,22
PRIMER_RIGHT_1=305,22
PRIMER_LEFT_1_TM=59.939
PRIMER_RIGHT_1_TM=60.117
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.1786
PRIMER_LEFT_2_PENALTY=0.061184
PRIMER_RIGHT_2_PENALTY=0.117452
PRIMER_LEFT_2_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_2_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT_2=128,22
PRIMER_RIGHT_2=335,22
PRIMER_LEFT_2_TM=59.939
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.1786
PRIMER_LEFT_3_PENALTY=0.061184
PRIMER_RIGHT_3_PENALTY=0.117452
PRIMER_LEFT_3_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_3_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT_3=128,22
PRIMER_RIGHT_3=365,22
PRIMER_LEFT_3_TM=59.939
PRIMER_RIGHT_3_TM=60.117
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.1848
PRIMER_LEFT_4_PENALTY=0.075241
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=ACCAACATTATTCCTCCCTCCT
PRIMER_RIGHT_4_SEQUENCE=ATCTTATTCTTGGTGGTGGTGG
PRIMER_LEFT_4=121,22
PRIMER_RIGHT_4=350,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_04403 Ps_contig_mira-and-tgicl-ass_franssen_33142
SEQUENCE=GGATCTTGAAAAAATCAATTGCGGCCGCCATGATTTCAATAATACCATTCATTTTAATTCACACTTTGTTTTATGTTGGATATTCTGATGATCTACCACTTACCACTACACTTCCACAATCACCAACATTATTCCTCCCTCCTTTTCACATATACCATCAAATATCCAAACCATATTCTTCATATGAACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCAAGGAGAAGATCTCCACCACCACCACCTAGGAGAAGATCCCCCCCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCTAGGATAAGATCTCCACCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCACCTAGGATAAGATCTCCACCACCTCCACCTCCATCACCACCACCACCTCCTCCATCACCACCACCACCACCACCACCTTCTCCATCACCACCACCACCTCTTTCATTTGACCATTTCGTGCTATCTCAGACATGGCCACCAACATATTGCAAGATTAAAAATAACAAATGTGTCTCTCCAGAGCCACTAAAGTTTGTCATTCACGGGTTGTGGCCAAGTAAAAAAGACACTCTGATACAAGATTGCGATGAAAAAATTATAGATGTTAATGACCTTGAGCCACTAATGGAAGAACTTAATGAAGATTGGCCAGCATTATTTGAAAATGATAACCAAACAGAAGCGAACATTCAACTATGGGTTGACCAGTGGAATGGTCACGGGACTTGTTCAGTTGAGTTGTTCGAATTTGTTCCTTACTTTGAAGAGACATTAAATGTTTACCATCGAAATAGTATAAAAGACATCCTTATAGAGAATGATATTAATCCGGGCGGAACGTTTCCTAAAGAAATTATTCTCAATGCAATACACACACATATTTTATTTATGCCACAACTTCGATGCAAGCGGATTAATGATACAGATTATTTATTTGAAATAAGACTTTGCTTGACAGCAAGTGTTGAACTAGAGTATAAAGATTGTGATACTCCTTATAGTGGTTGTCACAATGAGGATGTGTATTTTTAATAAATTATAATGTTATTTTATGGTCATGATGTCTGAGCTTTTGAAGTTCAAAGGATGCTACTAAAGCTTCATAGGAGTGTGAAGTTTGTCTCTTATTTTAGTTTTCTACTATGTAATTATTTTTCTTTTTTCCCGTGTTTTATTTGTTGGATTGTTGTTGTTTAATTTTAGTTACTAAACNCCTTC
TARGET=294,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1707
PRIMER_LEFT_PENALTY=0.061184
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_SEQUENCE=ATCTTATTCTTGGTGGTGGTGG
PRIMER_LEFT=128,22
PRIMER_RIGHT=350,22
PRIMER_LEFT_TM=59.939
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=223
PRIMER_PAIR_PENALTY_1=0.1786
PRIMER_LEFT_1_PENALTY=0.061184
PRIMER_RIGHT_1_PENALTY=0.117452
PRIMER_LEFT_1_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_1_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT_1=128,22
PRIMER_RIGHT_1=335,22
PRIMER_LEFT_1_TM=59.939
PRIMER_RIGHT_1_TM=60.117
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=0.1786
PRIMER_LEFT_2_PENALTY=0.061184
PRIMER_RIGHT_2_PENALTY=0.117452
PRIMER_LEFT_2_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_2_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT_2=128,22
PRIMER_RIGHT_2=365,22
PRIMER_LEFT_2_TM=59.939
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.1848
PRIMER_LEFT_3_PENALTY=0.075241
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=ACCAACATTATTCCTCCCTCCT
PRIMER_RIGHT_3_SEQUENCE=ATCTTATTCTTGGTGGTGGTGG
PRIMER_LEFT_3=121,22
PRIMER_RIGHT_3=350,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=0.1927
PRIMER_LEFT_4_PENALTY=0.075241
PRIMER_RIGHT_4_PENALTY=0.117452
PRIMER_LEFT_4_SEQUENCE=ACCAACATTATTCCTCCCTCCT
PRIMER_RIGHT_4_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT_4=121,22
PRIMER_RIGHT_4=335,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=60.117
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_04404 Ps_contig_mira-and-tgicl-ass_franssen_33142
SEQUENCE=GGATCTTGAAAAAATCAATTGCGGCCGCCATGATTTCAATAATACCATTCATTTTAATTCACACTTTGTTTTATGTTGGATATTCTGATGATCTACCACTTACCACTACACTTCCACAATCACCAACATTATTCCTCCCTCCTTTTCACATATACCATCAAATATCCAAACCATATTCTTCATATGAACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCAAGGAGAAGATCTCCACCACCACCACCTAGGAGAAGATCCCCCCCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCTAGGATAAGATCTCCACCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCACCTAGGATAAGATCTCCACCACCTCCACCTCCATCACCACCACCACCTCCTCCATCACCACCACCACCACCACCACCTTCTCCATCACCACCACCACCTCTTTCATTTGACCATTTCGTGCTATCTCAGACATGGCCACCAACATATTGCAAGATTAAAAATAACAAATGTGTCTCTCCAGAGCCACTAAAGTTTGTCATTCACGGGTTGTGGCCAAGTAAAAAAGACACTCTGATACAAGATTGCGATGAAAAAATTATAGATGTTAATGACCTTGAGCCACTAATGGAAGAACTTAATGAAGATTGGCCAGCATTATTTGAAAATGATAACCAAACAGAAGCGAACATTCAACTATGGGTTGACCAGTGGAATGGTCACGGGACTTGTTCAGTTGAGTTGTTCGAATTTGTTCCTTACTTTGAAGAGACATTAAATGTTTACCATCGAAATAGTATAAAAGACATCCTTATAGAGAATGATATTAATCCGGGCGGAACGTTTCCTAAAGAAATTATTCTCAATGCAATACACACACATATTTTATTTATGCCACAACTTCGATGCAAGCGGATTAATGATACAGATTATTTATTTGAAATAAGACTTTGCTTGACAGCAAGTGTTGAACTAGAGTATAAAGATTGTGATACTCCTTATAGTGGTTGTCACAATGAGGATGTGTATTTTTAATAAATTATAATGTTATTTTATGGTCATGATGTCTGAGCTTTTGAAGTTCAAAGGATGCTACTAAAGCTTCATAGGAGTGTGAAGTTTGTCTCTTATTTTAGTTTTCTACTATGTAATTATTTTTCTTTTTTCCCGTGTTTTATTTGTTGGATTGTTGTTGTTTAATTTTAGTTACTAAACNCCTTC
TARGET=324,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1786
PRIMER_LEFT_PENALTY=0.061184
PRIMER_RIGHT_PENALTY=0.117452
PRIMER_LEFT_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT=128,22
PRIMER_RIGHT=365,22
PRIMER_LEFT_TM=59.939
PRIMER_RIGHT_TM=60.117
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.1927
PRIMER_LEFT_1_PENALTY=0.075241
PRIMER_RIGHT_1_PENALTY=0.117452
PRIMER_LEFT_1_SEQUENCE=ACCAACATTATTCCTCCCTCCT
PRIMER_RIGHT_1_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT_1=121,22
PRIMER_RIGHT_1=365,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=60.117
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.3044
PRIMER_LEFT_2_PENALTY=0.186949
PRIMER_RIGHT_2_PENALTY=0.117452
PRIMER_LEFT_2_SEQUENCE=TCACCAACATTATTCCTCCCTC
PRIMER_RIGHT_2_SEQUENCE=GTGGTGGTGGTGGAGATCTTAT
PRIMER_LEFT_2=119,22
PRIMER_RIGHT_2=365,22
PRIMER_LEFT_2_TM=60.187
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.3100
PRIMER_LEFT_3_PENALTY=0.061184
PRIMER_RIGHT_3_PENALTY=0.248820
PRIMER_LEFT_3_SEQUENCE=TTATTCCTCCCTCCTTTTCACA
PRIMER_RIGHT_3_SEQUENCE=TCTTATCCTAGGTGGTGGTGGT
PRIMER_LEFT_3=128,22
PRIMER_RIGHT_3=382,22
PRIMER_LEFT_3_TM=59.939
PRIMER_RIGHT_3_TM=59.751
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.3241
PRIMER_LEFT_4_PENALTY=0.075241
PRIMER_RIGHT_4_PENALTY=0.248820
PRIMER_LEFT_4_SEQUENCE=ACCAACATTATTCCTCCCTCCT
PRIMER_RIGHT_4_SEQUENCE=TCTTATCCTAGGTGGTGGTGGT
PRIMER_LEFT_4=121,22
PRIMER_RIGHT_4=382,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_04405 Ps_contig_mira-and-tgicl-ass_franssen_33142
SEQUENCE=GGATCTTGAAAAAATCAATTGCGGCCGCCATGATTTCAATAATACCATTCATTTTAATTCACACTTTGTTTTATGTTGGATATTCTGATGATCTACCACTTACCACTACACTTCCACAATCACCAACATTATTCCTCCCTCCTTTTCACATATACCATCAAATATCCAAACCATATTCTTCATATGAACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCAAGGAGAAGATCTCCACCACCACCACCTAGGAGAAGATCCCCCCCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCTAGGATAAGATCTCCACCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCACCTAGGATAAGATCTCCACCACCTCCACCTCCATCACCACCACCACCTCCTCCATCACCACCACCACCACCACCACCTTCTCCATCACCACCACCACCTCTTTCATTTGACCATTTCGTGCTATCTCAGACATGGCCACCAACATATTGCAAGATTAAAAATAACAAATGTGTCTCTCCAGAGCCACTAAAGTTTGTCATTCACGGGTTGTGGCCAAGTAAAAAAGACACTCTGATACAAGATTGCGATGAAAAAATTATAGATGTTAATGACCTTGAGCCACTAATGGAAGAACTTAATGAAGATTGGCCAGCATTATTTGAAAATGATAACCAAACAGAAGCGAACATTCAACTATGGGTTGACCAGTGGAATGGTCACGGGACTTGTTCAGTTGAGTTGTTCGAATTTGTTCCTTACTTTGAAGAGACATTAAATGTTTACCATCGAAATAGTATAAAAGACATCCTTATAGAGAATGATATTAATCCGGGCGGAACGTTTCCTAAAGAAATTATTCTCAATGCAATACACACACATATTTTATTTATGCCACAACTTCGATGCAAGCGGATTAATGATACAGATTATTTATTTGAAATAAGACTTTGCTTGACAGCAAGTGTTGAACTAGAGTATAAAGATTGTGATACTCCTTATAGTGGTTGTCACAATGAGGATGTGTATTTTTAATAAATTATAATGTTATTTTATGGTCATGATGTCTGAGCTTTTGAAGTTCAAAGGATGCTACTAAAGCTTCATAGGAGTGTGAAGTTTGTCTCTTATTTTAGTTTTCTACTATGTAATTATTTTTCTTTTTTCCCGTGTTTTATTTGTTGGATTGTTGTTGTTTAATTTTAGTTACTAAACNCCTTC
TARGET=354,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3305
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=0.220992
PRIMER_LEFT_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_SEQUENCE=AATGGTCAAATGAAAGAGGTGG
PRIMER_LEFT=329,22
PRIMER_RIGHT=485,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=60.221
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.3305
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.220992
PRIMER_LEFT_1_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_1_SEQUENCE=AATGGTCAAATGAAAGAGGTGG
PRIMER_LEFT_1=269,22
PRIMER_RIGHT_1=485,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.221
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.3311
PRIMER_LEFT_2_PENALTY=0.110131
PRIMER_RIGHT_2_PENALTY=0.220992
PRIMER_LEFT_2_SEQUENCE=AAGAGAAGATCTCCACCACCAA
PRIMER_RIGHT_2_SEQUENCE=AATGGTCAAATGAAAGAGGTGG
PRIMER_LEFT_2=203,22
PRIMER_RIGHT_2=485,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.221
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.3746
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.265053
PRIMER_LEFT_3_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_3_SEQUENCE=ATGGTCAAATGAAAGAGGTGGT
PRIMER_LEFT_3=329,22
PRIMER_RIGHT_3=484,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.735
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.3746
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.265053
PRIMER_LEFT_4_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_4_SEQUENCE=ATGGTCAAATGAAAGAGGTGGT
PRIMER_LEFT_4=269,22
PRIMER_RIGHT_4=484,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.735
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_04406 Ps_contig_mira-and-tgicl-ass_franssen_33142
SEQUENCE=GGATCTTGAAAAAATCAATTGCGGCCGCCATGATTTCAATAATACCATTCATTTTAATTCACACTTTGTTTTATGTTGGATATTCTGATGATCTACCACTTACCACTACACTTCCACAATCACCAACATTATTCCTCCCTCCTTTTCACATATACCATCAAATATCCAAACCATATTCTTCATATGAACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCAAGGAGAAGATCTCCACCACCACCACCTAGGAGAAGATCCCCCCCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCTAGGATAAGATCTCCACCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCACCTAGGATAAGATCTCCACCACCTCCACCTCCATCACCACCACCACCTCCTCCATCACCACCACCACCACCACCACCTTCTCCATCACCACCACCACCTCTTTCATTTGACCATTTCGTGCTATCTCAGACATGGCCACCAACATATTGCAAGATTAAAAATAACAAATGTGTCTCTCCAGAGCCACTAAAGTTTGTCATTCACGGGTTGTGGCCAAGTAAAAAAGACACTCTGATACAAGATTGCGATGAAAAAATTATAGATGTTAATGACCTTGAGCCACTAATGGAAGAACTTAATGAAGATTGGCCAGCATTATTTGAAAATGATAACCAAACAGAAGCGAACATTCAACTATGGGTTGACCAGTGGAATGGTCACGGGACTTGTTCAGTTGAGTTGTTCGAATTTGTTCCTTACTTTGAAGAGACATTAAATGTTTACCATCGAAATAGTATAAAAGACATCCTTATAGAGAATGATATTAATCCGGGCGGAACGTTTCCTAAAGAAATTATTCTCAATGCAATACACACACATATTTTATTTATGCCACAACTTCGATGCAAGCGGATTAATGATACAGATTATTTATTTGAAATAAGACTTTGCTTGACAGCAAGTGTTGAACTAGAGTATAAAGATTGTGATACTCCTTATAGTGGTTGTCACAATGAGGATGTGTATTTTTAATAAATTATAATGTTATTTTATGGTCATGATGTCTGAGCTTTTGAAGTTCAAAGGATGCTACTAAAGCTTCATAGGAGTGTGAAGTTTGTCTCTTATTTTAGTTTTCTACTATGTAATTATTTTTCTTTTTTCCCGTGTTTTATTTGTTGGATTGTTGTTGTTTAATTTTAGTTACTAAACNCCTTC
TARGET=406,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3305
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=0.220992
PRIMER_LEFT_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_SEQUENCE=AATGGTCAAATGAAAGAGGTGG
PRIMER_LEFT=329,22
PRIMER_RIGHT=485,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=60.221
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.3305
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.220992
PRIMER_LEFT_1_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_1_SEQUENCE=AATGGTCAAATGAAAGAGGTGG
PRIMER_LEFT_1=269,22
PRIMER_RIGHT_1=485,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.221
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.3311
PRIMER_LEFT_2_PENALTY=0.110131
PRIMER_RIGHT_2_PENALTY=0.220992
PRIMER_LEFT_2_SEQUENCE=AAGAGAAGATCTCCACCACCAA
PRIMER_RIGHT_2_SEQUENCE=AATGGTCAAATGAAAGAGGTGG
PRIMER_LEFT_2=203,22
PRIMER_RIGHT_2=485,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.221
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.3746
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.265053
PRIMER_LEFT_3_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_3_SEQUENCE=ATGGTCAAATGAAAGAGGTGGT
PRIMER_LEFT_3=329,22
PRIMER_RIGHT_3=484,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.735
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.3746
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.265053
PRIMER_LEFT_4_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_4_SEQUENCE=ATGGTCAAATGAAAGAGGTGGT
PRIMER_LEFT_4=269,22
PRIMER_RIGHT_4=484,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.735
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_04407 Ps_contig_mira-and-tgicl-ass_franssen_33142
SEQUENCE=GGATCTTGAAAAAATCAATTGCGGCCGCCATGATTTCAATAATACCATTCATTTTAATTCACACTTTGTTTTATGTTGGATATTCTGATGATCTACCACTTACCACTACACTTCCACAATCACCAACATTATTCCTCCCTCCTTTTCACATATACCATCAAATATCCAAACCATATTCTTCATATGAACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCAAGGAGAAGATCTCCACCACCACCACCTAGGAGAAGATCCCCCCCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCTAGGATAAGATCTCCACCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCACCTAGGATAAGATCTCCACCACCTCCACCTCCATCACCACCACCACCTCCTCCATCACCACCACCACCACCACCACCTTCTCCATCACCACCACCACCTCTTTCATTTGACCATTTCGTGCTATCTCAGACATGGCCACCAACATATTGCAAGATTAAAAATAACAAATGTGTCTCTCCAGAGCCACTAAAGTTTGTCATTCACGGGTTGTGGCCAAGTAAAAAAGACACTCTGATACAAGATTGCGATGAAAAAATTATAGATGTTAATGACCTTGAGCCACTAATGGAAGAACTTAATGAAGATTGGCCAGCATTATTTGAAAATGATAACCAAACAGAAGCGAACATTCAACTATGGGTTGACCAGTGGAATGGTCACGGGACTTGTTCAGTTGAGTTGTTCGAATTTGTTCCTTACTTTGAAGAGACATTAAATGTTTACCATCGAAATAGTATAAAAGACATCCTTATAGAGAATGATATTAATCCGGGCGGAACGTTTCCTAAAGAAATTATTCTCAATGCAATACACACACATATTTTATTTATGCCACAACTTCGATGCAAGCGGATTAATGATACAGATTATTTATTTGAAATAAGACTTTGCTTGACAGCAAGTGTTGAACTAGAGTATAAAGATTGTGATACTCCTTATAGTGGTTGTCACAATGAGGATGTGTATTTTTAATAAATTATAATGTTATTTTATGGTCATGATGTCTGAGCTTTTGAAGTTCAAAGGATGCTACTAAAGCTTCATAGGAGTGTGAAGTTTGTCTCTTATTTTAGTTTTCTACTATGTAATTATTTTTCTTTTTTCCCGTGTTTTATTTGTTGGATTGTTGTTGTTTAATTTTAGTTACTAAACNCCTTC
TARGET=427,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3305
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=0.220992
PRIMER_LEFT_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_SEQUENCE=AATGGTCAAATGAAAGAGGTGG
PRIMER_LEFT=329,22
PRIMER_RIGHT=485,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=60.221
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.3305
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.220992
PRIMER_LEFT_1_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_1_SEQUENCE=AATGGTCAAATGAAAGAGGTGG
PRIMER_LEFT_1=269,22
PRIMER_RIGHT_1=485,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.221
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.3311
PRIMER_LEFT_2_PENALTY=0.110131
PRIMER_RIGHT_2_PENALTY=0.220992
PRIMER_LEFT_2_SEQUENCE=AAGAGAAGATCTCCACCACCAA
PRIMER_RIGHT_2_SEQUENCE=AATGGTCAAATGAAAGAGGTGG
PRIMER_LEFT_2=203,22
PRIMER_RIGHT_2=485,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.221
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.3746
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.265053
PRIMER_LEFT_3_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_3_SEQUENCE=ATGGTCAAATGAAAGAGGTGGT
PRIMER_LEFT_3=329,22
PRIMER_RIGHT_3=484,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.735
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.3746
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.265053
PRIMER_LEFT_4_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_4_SEQUENCE=ATGGTCAAATGAAAGAGGTGGT
PRIMER_LEFT_4=269,22
PRIMER_RIGHT_4=484,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.735
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_04408 Ps_contig_mira-and-tgicl-ass_franssen_33142
SEQUENCE=GGATCTTGAAAAAATCAATTGCGGCCGCCATGATTTCAATAATACCATTCATTTTAATTCACACTTTGTTTTATGTTGGATATTCTGATGATCTACCACTTACCACTACACTTCCACAATCACCAACATTATTCCTCCCTCCTTTTCACATATACCATCAAATATCCAAACCATATTCTTCATATGAACCTCTAGAGGAAAGTAAGAGAAGATCTCCACCACCAAGGAGAAGATCTCCACCACCACCACCTAGGAGAAGATCCCCCCCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCTAGGATAAGATCTCCACCACCACCACCACCAAGAATAAGATCTCCACCACCACCACCACCACCTAGGATAAGATCTCCACCACCTCCACCTCCATCACCACCACCACCTCCTCCATCACCACCACCACCACCACCACCTTCTCCATCACCACCACCACCTCTTTCATTTGACCATTTCGTGCTATCTCAGACATGGCCACCAACATATTGCAAGATTAAAAATAACAAATGTGTCTCTCCAGAGCCACTAAAGTTTGTCATTCACGGGTTGTGGCCAAGTAAAAAAGACACTCTGATACAAGATTGCGATGAAAAAATTATAGATGTTAATGACCTTGAGCCACTAATGGAAGAACTTAATGAAGATTGGCCAGCATTATTTGAAAATGATAACCAAACAGAAGCGAACATTCAACTATGGGTTGACCAGTGGAATGGTCACGGGACTTGTTCAGTTGAGTTGTTCGAATTTGTTCCTTACTTTGAAGAGACATTAAATGTTTACCATCGAAATAGTATAAAAGACATCCTTATAGAGAATGATATTAATCCGGGCGGAACGTTTCCTAAAGAAATTATTCTCAATGCAATACACACACATATTTTATTTATGCCACAACTTCGATGCAAGCGGATTAATGATACAGATTATTTATTTGAAATAAGACTTTGCTTGACAGCAAGTGTTGAACTAGAGTATAAAGATTGTGATACTCCTTATAGTGGTTGTCACAATGAGGATGTGTATTTTTAATAAATTATAATGTTATTTTATGGTCATGATGTCTGAGCTTTTGAAGTTCAAAGGATGCTACTAAAGCTTCATAGGAGTGTGAAGTTTGTCTCTTATTTTAGTTTTCTACTATGTAATTATTTTTCTTTTTTCCCGTGTTTTATTTGTTGGATTGTTGTTGTTTAATTTTAGTTACTAAACNCCTTC
TARGET=457,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3798
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=0.270338
PRIMER_LEFT_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_SEQUENCE=TGTCTGAGATAGCACGAAATGG
PRIMER_LEFT=329,22
PRIMER_RIGHT=502,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=60.270
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=0.3798
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.270338
PRIMER_LEFT_1_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_1_SEQUENCE=TGTCTGAGATAGCACGAAATGG
PRIMER_LEFT_1=269,22
PRIMER_RIGHT_1=502,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.270
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.3798
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.270338
PRIMER_LEFT_2_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_2_SEQUENCE=CTGAGATAGCACGAAATGGTCA
PRIMER_LEFT_2=329,22
PRIMER_RIGHT_2=499,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.270
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.3798
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.270338
PRIMER_LEFT_3_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_3_SEQUENCE=CTGAGATAGCACGAAATGGTCA
PRIMER_LEFT_3=269,22
PRIMER_RIGHT_3=499,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=60.270
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=231
PRIMER_PAIR_PENALTY_4=0.3798
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.270338
PRIMER_LEFT_4_SEQUENCE=CCACCACCACCAAGAATAAGAT
PRIMER_RIGHT_4_SEQUENCE=CCATGTCTGAGATAGCACGAAA
PRIMER_LEFT_4=329,22
PRIMER_RIGHT_4=505,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=60.270
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_04409 Ps_contig_mira-and-tgicl-ass_franssen_33145
SEQUENCE=CCTTTCTTTACTTTACCGGTAAACCGGGTTTACCTTTCCCCCTCGTNTGCCTTCAATTCCAGGAACTCTGAAATTCCATCCATGTCTCCCTTTACTATGTCAATGGCTGAATCGAACAAGCCCAGGTGGCGAAGGGTTTTGCTCAAAGTTAGTGGAGAAGCACTTGCAGGAGATCAGGCTCAGAATATTGACCCTAAGATAACTATGGCTATTGCAAGGGAAGTAGCAGCTGTAACTCGCCTTGGGATCGAGGTTGCACTTGTAGTTGGTGGAGGTAACATCTTCCGTGGAGCCTCCTGGGCTGGAAGTAGTGGTCTTGACCGCTCATCTGCTGATTATATTGGGATGTTGGCTACTGTTATGAATGCTATATTTCTTCAAGCAACAATGGAGAGTATGGGCATTCCAACTAGGGTGCAGACTGCATTTCGCATGTCTGAGGTTGCAGAACCATACATACGCAGAAGGGCTGTCAGGCATTTGGAAAAAGGAAGGGTTGTCATTTTTGCTGCTGGAACTGGAAATCCATTCTTTACCACAGACACCGCCGCAGCACTTAGATGTGCAGAAATTAATGCAGAGGTTGTACTCAAAGCTACAAATGTTGATGGAGTATTTGATGATGACCCAAAGCGTAACCCTCAAGCGCGTCTTCTTGACACATTAACCTATCAGGATGTAATAACAAAAAATCTGTCAGTGATGGATCTTACTGCCGTAACATTATGTCAGGAAAATAATATTCCTGTTGTTGTCTTCAATCTAAACAAACCAGGTAACATAGAGAAAGCCATTAGAGGGGAGAGAGTTGGAACTTTGATTGGGGCAACGTGGAATTCTACCGTATCAAGAACGTGATAATATTTGAAGACCAATTACAAGTGATTCTTTCTCGTGGTTTTCTTATGCAATCTTCCAGCTGAGGATGCCAAAAAGGCAAGGTGCATTATTTTGCAGGTGGTGGCTTTGATGTACTCTAAGGACACTGTTTTGAGAATCTGTAAATATAACATAGGTATATCAATTTTATTTGCTATCCACGTGGTTTGGTAACCTTATCTTGCCATATTATGTGGTTTTTTCTTTCTTTCTGAATAGAAATTTTTACTTCTACT
TARGET=1080,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04410 Ps_contig_mira-and-tgicl-ass_franssen_33157
SEQUENCE=GTCGGGGTGAATCTAACTTTCTACAAAGAATCTCGTGAAACCCAAAATCTCTCTCAACCCAAATCGATCATTCTAGAATCTCATGACTCAATCCATGTCTCACAAACCCTCCATTGACTCATGCGTGTTACACCTCCGTAACTGGAAACCCTTCCATCTTCACCAAAAAACCCCACCCTTAGACACCCCCCACAACAACAACAAACGACCTTGTCTCTCCGACCGAACCACCACTTCTTTTTCCCTCGACCTCTCCAAACTAACCCTCGCCGATGAAGACAGACCAGTTAACAGAACCGGCAGTTACCGTCTCGTCGCGCGCAAGAGACGCCGTCGTGGCTCCAGATCTGTTTCCGGCCGTAGTAGCGATCGGAGTGCTACCAGGCGATGCTGTTCCGTTGGGGCTTCTGCTGCTTATGGAACTTGCTCTGATTTTCCGGTGGCTATGGGGACGGATTCGAGTGGGGAGGCTTTTTGGTAATGGGGATGCGAATTGGTCTTCTGATGTGAGTGAAGCTAAAAATTCGAGGGATTGTGGGAGTGGGGAGAAGGAGAAGGAGAATGTGGGTGTTGGGTTTGGGGTCAATGGCTGTTCTGATGCGAATGGGAATGAATCTGGGTATGGGAGTGAACCGGGTTATCGCGGAGATGCTGAATTTGGGTATGGGGATGAGTTTGATGAAGAAGAGGATGATCACAGAGTCTTGTTTTGGGGAAACCAACTCGGAGGAGGTACAGATTCAAAAATGGAGATGGTTGGGGAAAACACCTTGCTAGATCAAAAATCTCATCATAGATGCCGCCGTAGAAAGAATGATTGTAGAATGATTGATGCGTTAAGGTGATGATTGCACACAATTTTACCCTTCCCTTCAAAAGGAGACTGGGATTGGCTTCTTTTTGTGCATATGAATGAACTATGTCATCTTCGTCCTGACCCATTTGCCAAACCAATATATATCCTTTGTATTTTAGATGCCATGTGATATTTGGTTTGGGATCATTCAAGTTCTTAACCATCTGATCCAGTTGTTTGGGATTTGGTACATGTGTAACACTTTTAATCTCAGTCATTAAAAATTCAAAGAAAAAGATTTGAAACCAAAAATCTAAGTATTACCTTTGATGTTGCTAGCAAACAAAACTATATGATACTCAAGTTTGGATTATGGTGCGTGCGTCTTGGTTTTTAGCTATTTCTTGCTTGGTATAGAAAATTGTAATTTTAAACTCTATCTGGACTCCAAATTAACTCTTGACCTGGGCATTTTTTCAAAGT
TARGET=192,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3229
PRIMER_LEFT_PENALTY=0.274768
PRIMER_RIGHT_PENALTY=0.048131
PRIMER_LEFT_SEQUENCE=TACACCTCCGTAACTGGAAACC
PRIMER_RIGHT_SEQUENCE=AAGTTCCATAAGCAGCAGAAGC
PRIMER_LEFT=128,22
PRIMER_RIGHT=424,22
PRIMER_LEFT_TM=60.275
PRIMER_RIGHT_TM=60.048
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.3430
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.233451
PRIMER_LEFT_1_SEQUENCE=CTCGTGAAACCCAAAATCTCTC
PRIMER_RIGHT_1_SEQUENCE=AGAGGTCGAGGGAAAAAGAAGT
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=253,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=59.767
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.3430
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.233451
PRIMER_LEFT_2_SEQUENCE=TCGTGAAACCCAAAATCTCTCT
PRIMER_RIGHT_2_SEQUENCE=AGAGGTCGAGGGAAAAAGAAGT
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=253,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.767
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.3430
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.233451
PRIMER_LEFT_3_SEQUENCE=CGTGAAACCCAAAATCTCTCTC
PRIMER_RIGHT_3_SEQUENCE=AGAGGTCGAGGGAAAAAGAAGT
PRIMER_LEFT_3=33,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.767
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.3430
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.233451
PRIMER_LEFT_4_SEQUENCE=TCTCGTGAAACCCAAAATCTCT
PRIMER_RIGHT_4_SEQUENCE=AGAGGTCGAGGGAAAAAGAAGT
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=253,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.767
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_04411 Ps_contig_mira-and-tgicl-ass_franssen_33164
SEQUENCE=AATAAAGTTNGAGGTTGGTGCTGTTGTTGGCGATTGATATTGATTCTCTTCCCCCTTCTTCTCCTATCTCTCTCTCCTTCCTCGTCGCACAAATCGTATCAGCAGCAATGTCTGGAGAATCCGCTACCGCTATTCACAAGGGTCAAGTTGATCTCTTAGACTTCATAGATTGGACTGGTGTTGAATGCCTCAATCAAAGCACCACTCACTCTCTTCCCAATGCTATCAAACAGGGTTATAGAGAAGATGAAGGTTTGCATCTAGAAAGTGATGCAGATGAACAGCTTTTGCTTTATATTCCTTTCACCCAAGTTATTAAACTTTATTCCATTGTTATCAAAGGTCCTGAAGATGAAGGCCCCAAGACAGTGAAGCTCTTTTCCAACAAGGAGCACATGGGATTCAGTAACGTAAATGACTATCCACCAAGCGACACAGCTGTTTTGTCCCCAGAAAACCTCAAGGGGAAACCAGTACTTTTAAAGTATGTTAAATTTCAAAATGTTCGCAGCTTGACAATATTTATTGAGGATAATCAGACTGATTCCGAGATCACCAAAGTTCAGAAGATTCTGCTGATCGGATCAACAGTTGAAACAACCGACATGAAGGGTTTGAAGAAGATTGAGGATCATTGACCTCGTTAAAGTATTGAGTCTAGTTTCCTCTACATTCACCCTTATAAATTTTGATTGGAAATGTTTTATTCAGCTTCATGAATAATGGTTTATGACTTCTAACATCTATATTTTTTAGTAAGAGACTGAAGCTCAATATATCTGCATGGTTGTAACATTTATGTGATAGGAAGTAACGTTCAAGCTGCATGGCTGTTTATTTTCTTTTTCAGTGTTTGGGAAATATGTAAAAAATTGCGAAGTTGGACTACATTGATCGAGACATATAATTTGGTTATAACTAGGATTTAAGCAAAGTTATTAAAGT
TARGET=67,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4058
PRIMER_LEFT_PENALTY=0.328475
PRIMER_RIGHT_PENALTY=0.077340
PRIMER_LEFT_SEQUENCE=GATTCTCTTCCCCCTTCTTCTC
PRIMER_RIGHT_SEQUENCE=GGAAGAGAGTGAGTGGTGCTTT
PRIMER_LEFT=41,22
PRIMER_RIGHT=216,22
PRIMER_LEFT_TM=59.672
PRIMER_RIGHT_TM=59.923
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.4202
PRIMER_LEFT_1_PENALTY=0.342857
PRIMER_RIGHT_1_PENALTY=0.077340
PRIMER_LEFT_1_SEQUENCE=AAAGTTNGAGGTTGGTGCTGTT
PRIMER_RIGHT_1_SEQUENCE=GGAAGAGAGTGAGTGGTGCTTT
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=216,22
PRIMER_LEFT_1_TM=60.343
PRIMER_RIGHT_1_TM=59.923
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=0.4384
PRIMER_LEFT_2_PENALTY=0.342857
PRIMER_RIGHT_2_PENALTY=0.095523
PRIMER_LEFT_2_SEQUENCE=AAAGTTNGAGGTTGGTGCTGTT
PRIMER_RIGHT_2_SEQUENCE=CTCCAGACATTGCTGCTGATAC
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=116,22
PRIMER_LEFT_2_TM=60.343
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.3000
PRIMER_PAIR_2_COMPL_ANY=4.75
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=0.4400
PRIMER_LEFT_3_PENALTY=0.328475
PRIMER_RIGHT_3_PENALTY=0.111534
PRIMER_LEFT_3_SEQUENCE=GATTCTCTTCCCCCTTCTTCTC
PRIMER_RIGHT_3_SEQUENCE=GCTGTTCATCTGCATCACTTTC
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=284,22
PRIMER_LEFT_3_TM=59.672
PRIMER_RIGHT_3_TM=59.888
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.4537
PRIMER_LEFT_4_PENALTY=0.376347
PRIMER_RIGHT_4_PENALTY=0.077340
PRIMER_LEFT_4_SEQUENCE=CTGTTGTTGGCGATTGATATTG
PRIMER_RIGHT_4_SEQUENCE=GGAAGAGAGTGAGTGGTGCTTT
PRIMER_LEFT_4=20,22
PRIMER_RIGHT_4=216,22
PRIMER_LEFT_4_TM=60.376
PRIMER_RIGHT_4_TM=59.923
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=197
=
PRIMER_SEQUENCE_ID=SSR_04412 Ps_contig_mira-and-tgicl-ass_franssen_33166
SEQUENCE=CTTAACAAAGAAAACAATTTAATTYYTTTTAACATTAGAACCGAAAGTTCCACTAATTTACAATGCAACAAAATGGGGNTACAAAATCCGCCATTTCTATCCTTAAAAAGCCACAACCATTTCAGGTACGAAATGAATATTGTTGCCAAGTTGCCATTGTTCTCACTTTATTACGCACACACTCACTCACTTTTAGAACTCTTCACAAAAAAGGTAAATACTAAACATAATCAAAGATACATATATGCATATTACAACAACAACAACAAGATGGACATGAATCTATTCAGTATCTGAAATCACTGTGATTCTGTCATATTTTCTTTGAATCCAAACGTCCACCTAGTAACCGGACCACGACCAAAGAGCGTGATGATGATGGTCGTCCTCTTGAAATCCATAACCAGACGAGTTATGATGCGGGTTGGAGTAATGAGCATCTTCAAGTTTTGTAAAGATGTTATAAGGAGGCAACAAATTATCTTCATCATCTTGTGATAGGTCTGATTGATATTCAGGTTCAAACACATTTCTCTGCAACACTCTATCCTTGTAACATGGACTGTCACAATCCAATATGTCACTCCTTGCTGAACTTATATCTTCTTGTCTGCTATTATTATTATGATTACTTTCATTACAAGCCTCAACAATCGACAACTTTGATCCTTCACCCTCAGAAACTGAATCAACCAATGGCTTATTCATTACTGGTTCCTGCAGTAGTTTCTTTGTTTCACTTTCCCTCTCTTGAGTCTCTCTTGCAAGTACCTTCTTAGAGAGACTTTCCACCTCAGATTGTAACCTATGTTTCTCCTTGAGGATGTTATCATAATCAGTCTTAAGAGACTCATAACTATCATTAAGAGATTCATAATCCTTCTCAAGCTGTTTGTTCTTCCATCTTGCACGACGGTTTTGAAACCAAATAGCAACTTGCCGTGGCTGCAAACCGAGATCTTTAGCAATCTTGGTTTTCCTATCCGGTTCAAGCTTGTTCTCAACCTCAAAGCTTTTCTCAAGAAACTGAACTTGTTCCGCGGAGAGTCGCCGCTTCTTCTCCGATTGTTGAAAGTAGTCATCCATCTCATCGTCTCCGTTTTCGTCTAGATCGAATCCGTTGAAGAGATAACTGTTGCACCTTTTCCTTCCTCCTTGAGCATCTTCAAAACTCACCATAGTTCTTGAACCGAAGAAAGGAAGAGAAGAAGAAAGAAAGAGAGAATCAAGAGGTTGTTGAGAAGTGTTTTGTTGTTGACTATGAAACAAAGTATTCATGTTTGAATTACTTGCAGGAGAGTTTGTGAAGAGTCTTTCACCAGCCATGTATATAAAACAAAGGTAAATTCAAGGAAGTTTGTTTTTGTTTTTGTTTTTGTTATCTTAACGTGCCATAGGTATAGGAACGAGGTATAGGTAGAAGGAAGGAAAGAAAGATCTAAGACTTATGGCGAAAGAAACTGGTGATGATGATTTCAGTGATGTGAGACTGGGAGGTGTGGGAAGAACGGCAGGGGATACCCATATCTCCGATCAGAAGAAGGCGGTGAAGGGAGACCCGATTGAGTGTTTCGATGGGGGGAGAGAATCAAACAAGATGGACAGAGAGACAGGGACAGAAGCGGTGGTGGCTATTTTTCTTTCTGGCTGGATTTGTTTTTTGTCCCCACGTA
TARGET=180,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04413 Ps_contig_mira-and-tgicl-ass_franssen_33166
SEQUENCE=CTTAACAAAGAAAACAATTTAATTYYTTTTAACATTAGAACCGAAAGTTCCACTAATTTACAATGCAACAAAATGGGGNTACAAAATCCGCCATTTCTATCCTTAAAAAGCCACAACCATTTCAGGTACGAAATGAATATTGTTGCCAAGTTGCCATTGTTCTCACTTTATTACGCACACACTCACTCACTTTTAGAACTCTTCACAAAAAAGGTAAATACTAAACATAATCAAAGATACATATATGCATATTACAACAACAACAACAAGATGGACATGAATCTATTCAGTATCTGAAATCACTGTGATTCTGTCATATTTTCTTTGAATCCAAACGTCCACCTAGTAACCGGACCACGACCAAAGAGCGTGATGATGATGGTCGTCCTCTTGAAATCCATAACCAGACGAGTTATGATGCGGGTTGGAGTAATGAGCATCTTCAAGTTTTGTAAAGATGTTATAAGGAGGCAACAAATTATCTTCATCATCTTGTGATAGGTCTGATTGATATTCAGGTTCAAACACATTTCTCTGCAACACTCTATCCTTGTAACATGGACTGTCACAATCCAATATGTCACTCCTTGCTGAACTTATATCTTCTTGTCTGCTATTATTATTATGATTACTTTCATTACAAGCCTCAACAATCGACAACTTTGATCCTTCACCCTCAGAAACTGAATCAACCAATGGCTTATTCATTACTGGTTCCTGCAGTAGTTTCTTTGTTTCACTTTCCCTCTCTTGAGTCTCTCTTGCAAGTACCTTCTTAGAGAGACTTTCCACCTCAGATTGTAACCTATGTTTCTCCTTGAGGATGTTATCATAATCAGTCTTAAGAGACTCATAACTATCATTAAGAGATTCATAATCCTTCTCAAGCTGTTTGTTCTTCCATCTTGCACGACGGTTTTGAAACCAAATAGCAACTTGCCGTGGCTGCAAACCGAGATCTTTAGCAATCTTGGTTTTCCTATCCGGTTCAAGCTTGTTCTCAACCTCAAAGCTTTTCTCAAGAAACTGAACTTGTTCCGCGGAGAGTCGCCGCTTCTTCTCCGATTGTTGAAAGTAGTCATCCATCTCATCGTCTCCGTTTTCGTCTAGATCGAATCCGTTGAAGAGATAACTGTTGCACCTTTTCCTTCCTCCTTGAGCATCTTCAAAACTCACCATAGTTCTTGAACCGAAGAAAGGAAGAGAAGAAGAAAGAAAGAGAGAATCAAGAGGTTGTTGAGAAGTGTTTTGTTGTTGACTATGAAACAAAGTATTCATGTTTGAATTACTTGCAGGAGAGTTTGTGAAGAGTCTTTCACCAGCCATGTATATAAAACAAAGGTAAATTCAAGGAAGTTTGTTTTTGTTTTTGTTTTTGTTATCTTAACGTGCCATAGGTATAGGAACGAGGTATAGGTAGAAGGAAGGAAAGAAAGATCTAAGACTTATGGCGAAAGAAACTGGTGATGATGATTTCAGTGATGTGAGACTGGGAGGTGTGGGAAGAACGGCAGGGGATACCCATATCTCCGATCAGAAGAAGGCGGTGAAGGGAGACCCGATTGAGTGTTTCGATGGGGGGAGAGAATCAAACAAGATGGACAGAGAGACAGGGACAGAAGCGGTGGTGGCTATTTTTCTTTCTGGCTGGATTTGTTTTTTGTCCCCACGTA
TARGET=254,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04414 Ps_contig_mira-and-tgicl-ass_franssen_33166
SEQUENCE=CTTAACAAAGAAAACAATTTAATTYYTTTTAACATTAGAACCGAAAGTTCCACTAATTTACAATGCAACAAAATGGGGNTACAAAATCCGCCATTTCTATCCTTAAAAAGCCACAACCATTTCAGGTACGAAATGAATATTGTTGCCAAGTTGCCATTGTTCTCACTTTATTACGCACACACTCACTCACTTTTAGAACTCTTCACAAAAAAGGTAAATACTAAACATAATCAAAGATACATATATGCATATTACAACAACAACAACAAGATGGACATGAATCTATTCAGTATCTGAAATCACTGTGATTCTGTCATATTTTCTTTGAATCCAAACGTCCACCTAGTAACCGGACCACGACCAAAGAGCGTGATGATGATGGTCGTCCTCTTGAAATCCATAACCAGACGAGTTATGATGCGGGTTGGAGTAATGAGCATCTTCAAGTTTTGTAAAGATGTTATAAGGAGGCAACAAATTATCTTCATCATCTTGTGATAGGTCTGATTGATATTCAGGTTCAAACACATTTCTCTGCAACACTCTATCCTTGTAACATGGACTGTCACAATCCAATATGTCACTCCTTGCTGAACTTATATCTTCTTGTCTGCTATTATTATTATGATTACTTTCATTACAAGCCTCAACAATCGACAACTTTGATCCTTCACCCTCAGAAACTGAATCAACCAATGGCTTATTCATTACTGGTTCCTGCAGTAGTTTCTTTGTTTCACTTTCCCTCTCTTGAGTCTCTCTTGCAAGTACCTTCTTAGAGAGACTTTCCACCTCAGATTGTAACCTATGTTTCTCCTTGAGGATGTTATCATAATCAGTCTTAAGAGACTCATAACTATCATTAAGAGATTCATAATCCTTCTCAAGCTGTTTGTTCTTCCATCTTGCACGACGGTTTTGAAACCAAATAGCAACTTGCCGTGGCTGCAAACCGAGATCTTTAGCAATCTTGGTTTTCCTATCCGGTTCAAGCTTGTTCTCAACCTCAAAGCTTTTCTCAAGAAACTGAACTTGTTCCGCGGAGAGTCGCCGCTTCTTCTCCGATTGTTGAAAGTAGTCATCCATCTCATCGTCTCCGTTTTCGTCTAGATCGAATCCGTTGAAGAGATAACTGTTGCACCTTTTCCTTCCTCCTTGAGCATCTTCAAAACTCACCATAGTTCTTGAACCGAAGAAAGGAAGAGAAGAAGAAAGAAAGAGAGAATCAAGAGGTTGTTGAGAAGTGTTTTGTTGTTGACTATGAAACAAAGTATTCATGTTTGAATTACTTGCAGGAGAGTTTGTGAAGAGTCTTTCACCAGCCATGTATATAAAACAAAGGTAAATTCAAGGAAGTTTGTTTTTGTTTTTGTTTTTGTTATCTTAACGTGCCATAGGTATAGGAACGAGGTATAGGTAGAAGGAAGGAAAGAAAGATCTAAGACTTATGGCGAAAGAAACTGGTGATGATGATTTCAGTGATGTGAGACTGGGAGGTGTGGGAAGAACGGCAGGGGATACCCATATCTCCGATCAGAAGAAGGCGGTGAAGGGAGACCCGATTGAGTGTTTCGATGGGGGGAGAGAATCAAACAAGATGGACAGAGAGACAGGGACAGAAGCGGTGGTGGCTATTTTTCTTTCTGGCTGGATTTGTTTTTTGTCCCCACGTA
TARGET=615,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04415 Ps_contig_mira-and-tgicl-ass_franssen_33166
SEQUENCE=CTTAACAAAGAAAACAATTTAATTYYTTTTAACATTAGAACCGAAAGTTCCACTAATTTACAATGCAACAAAATGGGGNTACAAAATCCGCCATTTCTATCCTTAAAAAGCCACAACCATTTCAGGTACGAAATGAATATTGTTGCCAAGTTGCCATTGTTCTCACTTTATTACGCACACACTCACTCACTTTTAGAACTCTTCACAAAAAAGGTAAATACTAAACATAATCAAAGATACATATATGCATATTACAACAACAACAACAAGATGGACATGAATCTATTCAGTATCTGAAATCACTGTGATTCTGTCATATTTTCTTTGAATCCAAACGTCCACCTAGTAACCGGACCACGACCAAAGAGCGTGATGATGATGGTCGTCCTCTTGAAATCCATAACCAGACGAGTTATGATGCGGGTTGGAGTAATGAGCATCTTCAAGTTTTGTAAAGATGTTATAAGGAGGCAACAAATTATCTTCATCATCTTGTGATAGGTCTGATTGATATTCAGGTTCAAACACATTTCTCTGCAACACTCTATCCTTGTAACATGGACTGTCACAATCCAATATGTCACTCCTTGCTGAACTTATATCTTCTTGTCTGCTATTATTATTATGATTACTTTCATTACAAGCCTCAACAATCGACAACTTTGATCCTTCACCCTCAGAAACTGAATCAACCAATGGCTTATTCATTACTGGTTCCTGCAGTAGTTTCTTTGTTTCACTTTCCCTCTCTTGAGTCTCTCTTGCAAGTACCTTCTTAGAGAGACTTTCCACCTCAGATTGTAACCTATGTTTCTCCTTGAGGATGTTATCATAATCAGTCTTAAGAGACTCATAACTATCATTAAGAGATTCATAATCCTTCTCAAGCTGTTTGTTCTTCCATCTTGCACGACGGTTTTGAAACCAAATAGCAACTTGCCGTGGCTGCAAACCGAGATCTTTAGCAATCTTGGTTTTCCTATCCGGTTCAAGCTTGTTCTCAACCTCAAAGCTTTTCTCAAGAAACTGAACTTGTTCCGCGGAGAGTCGCCGCTTCTTCTCCGATTGTTGAAAGTAGTCATCCATCTCATCGTCTCCGTTTTCGTCTAGATCGAATCCGTTGAAGAGATAACTGTTGCACCTTTTCCTTCCTCCTTGAGCATCTTCAAAACTCACCATAGTTCTTGAACCGAAGAAAGGAAGAGAAGAAGAAAGAAAGAGAGAATCAAGAGGTTGTTGAGAAGTGTTTTGTTGTTGACTATGAAACAAAGTATTCATGTTTGAATTACTTGCAGGAGAGTTTGTGAAGAGTCTTTCACCAGCCATGTATATAAAACAAAGGTAAATTCAAGGAAGTTTGTTTTTGTTTTTGTTTTTGTTATCTTAACGTGCCATAGGTATAGGAACGAGGTATAGGTAGAAGGAAGGAAAGAAAGATCTAAGACTTATGGCGAAAGAAACTGGTGATGATGATTTCAGTGATGTGAGACTGGGAGGTGTGGGAAGAACGGCAGGGGATACCCATATCTCCGATCAGAAGAAGGCGGTGAAGGGAGACCCGATTGAGTGTTTCGATGGGGGGAGAGAATCAAACAAGATGGACAGAGAGACAGGGACAGAAGCGGTGGTGGCTATTTTTCTTTCTGGCTGGATTTGTTTTTTGTCCCCACGTA
TARGET=1209,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04416 Ps_contig_mira-and-tgicl-ass_franssen_33174
SEQUENCE=AGATCTAAACACCGCAACTGCAACGCAACACTGAGATTTAAATTACTCCATTTACAATATTTAGATCTTAATCTTAATAACTTCATCATTTCATTTCATCATTAGGGTTTCATCGCGAACATGGGAGAACCAAAGCTAATCCCTCTGGCAATTTTTCCACTGCTCTTCTTCGTGATTTCTACCTCATCGTTACACCTTCTCACTGCTTCCGATGCCGATGACGCGATCTTCTACGAATCATTTGACGAGGCTTTTGATGGTCGTTGGATCCTTTCCGATAAGGATGAATACAACGGTGTTTGGGAGCATGCAAAGAGCGAGGGTCATGAAGATTACGGGCTTCTTGTCAGCCAGAAAGCAAGAAAATATGCGATAGTAAAAGAACTTGATGAGCCTGTGAGTCTTAAGGATAAATCAGTTGTTCTTCAGTTTGAGACTCGCCTTCAGAATGGCCTCGAGTGTGGTGGTGCATACATAAAATATCTTCGACCACAGGAGGCCGGTTGGAAGCCCAAGGAATTTGGCAATGATTCTCCATATTCAATTATGTTTGGTCCTGACAAGTGTGGAGCCACAAACAAGGTGCATTTCATTTTCAAGCATAAGAATCCCAAGAGTGGGGAGTATGTTGAACATCATCTCAAGTTTCCTCCATCCGTTCCTTCTGACAAATTATCTCATGTGTACACCGCTATTTTAAAACCAGACAACAAGTTACAAATTTTGATTGATGGAGAAGAGAAGAAAAAAGCAAATTTCTTATCTTCTGAGGATTTCGAGCCTTCCCTTATCCCTCCCAAGACAATCCCTGATCCTGAAGATAAGAAACCTGAAGACTGGGACGAGAGAGAGAAAATTCCAGACCCAAATGCAGTAAAGCCAGATGACTGGGATGAAGATGCACCCTTGGAAATTGTTGATGACGAAGCTGAAAAACCTGAAGGATGGTTGGATGATGAGCCTGAGGAGGTAGATGACCCAGAAGCTACAAGACCAGAAGATTGGGACGATGAGGAGGATGGTGAATGGGAAGCCCCAAAGGTTACCAACCCAAAGTGTGAAGCAGCCCCTGGTTGTGGTGAGTGGAAGAGGCCAATGAAGAAGAATCCAGCATATAAGGGAAAATGNCATGCTCCCCTTATTGAAAATCCAGCYTATAAGGGTATATGGAAGCCTCAGGATATTCCAAACCCGGATTACT
TARGET=844,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04417 Ps_contig_mira-and-tgicl-ass_franssen_33177
SEQUENCE=AACTTAAACCAACTGGTAACAATATACCAATATAATTTCATAAGTACCTTGCACATTATAATACTTTCTCAGTTATACGGATCAATATAAACATTACTGCATTCATCAAAACTGCTAGAGAAATTCTTGCCCAGATTAAACTAGTCATCATTAATTAAAAACAAAATTTTAACAATGTCTTAAACAAAATAAACCGCAACAATAAAAAAACATGTAAACCAAACTCGTCCTCATAATTTTTCCCTAAACAAAATCTGAAAAATAAACACCTCGAATTCGCTTATGTATTAGTGGCTCTGGTGTAAATCTGTTGGCTAGCATGAGCAGAAACCAACCTAATCAAACCTCTTGCCATTAGGTCTTTGATTGCACGACGTGCCAAAGATCCATTGACCCTAAGACGATCGGAAAGAACCGACGGAGTGATGAGTTTGTACTTGGGAGCTTCGGTGAGAAGCTTATCGTAGGTACCTTGGTCAAACAAGACCATGTTGTTGACCTTTTCCTTTTGCTTACCCTTGCTCCATTTCTTCTTCTTCTGTTTTCCTCCTCCGGATTTCGCCGGCTTTGACGACGGTGGTGGTGCCTTCTCCTTCTTCGGTGCCATTAGGGTTTGGAATGGGCGAGGTGGTAGAAGCCGTCAGAGAAGTGGGAGAAGGGTCCCCGANACTCGGTGTGATGAAATATAGGGTTTCAGATTTGTAATTCTTGGATCTTTTATGGGCCTGGGCTAAACCCGTATATATTAAAGTGGAGAAATATACCGAGCACCCTCCATGTTAGTTCTAGCACCCCATC
TARGET=528,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1389
PRIMER_LEFT_PENALTY=0.001341
PRIMER_RIGHT_PENALTY=0.137550
PRIMER_LEFT_SEQUENCE=CCTTTTCCTTTTGCTTACCCTT
PRIMER_RIGHT_SEQUENCE=GGTGCTCGGTATATTTCTCCAC
PRIMER_LEFT=498,22
PRIMER_RIGHT=771,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.862
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.1587
PRIMER_LEFT_1_PENALTY=0.021195
PRIMER_RIGHT_1_PENALTY=0.137550
PRIMER_LEFT_1_SEQUENCE=GTTGTTGACCTTTTCCTTTTGC
PRIMER_RIGHT_1_SEQUENCE=GGTGCTCGGTATATTTCTCCAC
PRIMER_LEFT_1=490,22
PRIMER_RIGHT_1=771,22
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=59.862
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=0.3579
PRIMER_LEFT_2_PENALTY=0.001341
PRIMER_RIGHT_2_PENALTY=0.356592
PRIMER_LEFT_2_SEQUENCE=CCTTTTCCTTTTGCTTACCCTT
PRIMER_RIGHT_2_SEQUENCE=AGAACTAACATGGAGGGTGCTC
PRIMER_LEFT_2=498,22
PRIMER_RIGHT_2=786,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=59.643
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.3645
PRIMER_LEFT_3_PENALTY=0.226971
PRIMER_RIGHT_3_PENALTY=0.137550
PRIMER_LEFT_3_SEQUENCE=ACCATGTTGTTGACCTTTTCCT
PRIMER_RIGHT_3_SEQUENCE=GGTGCTCGGTATATTTCTCCAC
PRIMER_LEFT_3=485,22
PRIMER_RIGHT_3=771,22
PRIMER_LEFT_3_TM=59.773
PRIMER_RIGHT_3_TM=59.862
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=0.3739
PRIMER_LEFT_4_PENALTY=0.001341
PRIMER_RIGHT_4_PENALTY=0.372557
PRIMER_LEFT_4_SEQUENCE=CCTTTTCCTTTTGCTTACCCTT
PRIMER_RIGHT_4_SEQUENCE=GGGGTGCTAGAACTAACATGGA
PRIMER_LEFT_4=498,22
PRIMER_RIGHT_4=794,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=60.373
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_04418 Ps_contig_mira-and-tgicl-ass_franssen_33180
SEQUENCE=ACTCTCTTCTATCTCCGTTTCCCCTTTCTCCTCTCTCAAACCCTTTTTTCCATTTTTGAAATTCAACCCATTTCACTTCCATGATATCACCCAATTCAATTTATAAATTCTAATCTTCTAATCTTCTAATCTTTTAATCTAGTCATTTCACAAATTTCCCCAACAAGATGAAGAGAGATCGTCAAGAAACCTCTGGTGGAGGTGTGAGTAGCAACAATAACAATAGCGACAACATCATCAGCAACAACTTTGTTAGTGTACGAGGTGAATGTTCATCAATGCAGAACAACAACAACAACGGAAAACAAAGTTTGTGGAGCGAAGAAAAAGAGAACAGTGGTGGAATGGACGAGTTACTAGCGGCATTAGGTTACAAGGTTCGTTCTTCAGACATGCTTGACGTAGCTCAAAAGCTTGAACAACTTGAGATGGTGATGGGTAGTGCTCAAGAGGAAGGAATTAACCATCTTGCTTCAGATACAGTTCACTACGATCCCACCGATCTATATTCATGGGTTCAAACCATGCTCACTGAACTTAACCCTTCCACCGATTCACAAATCAATGACCCTTTAGATTCTTCTTCTTCCATCTTCAACGATAACTCGCAGTATGATCTCAGTGTTATTCCTGGAATGGCCGCATACCCACCTCAGAGCCATAACCAAAACAGCGAAAGTGAAGGTTCTAACAGCAACAAAAGGTTGAAGACGTGGGGGAGTGAAACGGAATCGGAAGATATTTTTCTGCCGGCGTTATCACCGCCGGCGGAGACTACAAGACCGGTGGTTCTCGTTGACTCACAGGAAACTGGGGTTCGTCTTATTCACACTATGATGGCTTGCGCCGATGCGATTCAGCGTGATGATATCAAGATTGCTGATAGACTCGTGAAGAATATTGGAATATTGGCTTCGTCGCAAACCGGAGCGATGGGAAAAGTTGCTTCGTATTTCGCACAAGCTCTGTATCGGAGAATCTGCAGAGTTTCCCCTGATGAAACTCTGGATTCATCTTTGTCTGATGCACTTCACATGCACTTTTACGAGTCTTCTCCTTATCTCAAATTCGCTCACTTCACCGCTAATCAAGCTATTCTGGAAGCTTTTGCCGGTGCCGGAAGTGTTCACGTCATTGATTTTGGTCTCAAACAAGGGATGCAGTGGCCGGCGCTTATGCAAGCACTTGCATTACGTCCTGGTGGTCCACCAACTTTCCGGTTAACCGGAATCGGACCACCGCAGACGGGTAACACCGATGCTTTGCAGCAGGTGGGTTGGAAATTGGCTCAGCTTGCTCAGACCATTGGTGTTCAGTTCGAATTTCGTGGATTCGTTTGCAACAGTCTCGCGGATCTTGACCCGAATATGCTCGAGATCCGACCCGGTGAAGCCGTTGCTGTTAACTCCGTTTTCGAACTTCACACCATGTTAGCCCGACCCGGTTCCATTGATAAGGTTCTCAACACTGTTAAGAAGATTAACCCTAAGATTGTCACTATCGTGGAGCAAGAAGCGAATCATAACGGACCGGTTTTCATGGATCGGTTCACGGAGGCTTTGCATTATTACTCGAGTTTATTTGATTCTCTGGAGGGTTCTTCTAATTCAAACCCGGCCGGGTCTGGTTCTTCGAGTCAGGATCTGTTGATGTCTGAGCTTTACTTGGGGAGACAGATATGCAACGTGGTGGCTTATGAAGGTGTGGACCGGGTCGAGAGACACGAGACACTGAGTCAGTGGAGGTCGAGGATGGGTTCAGCTGGGTTCGACCCGGTTCATTTAGGTTCAAACGCGTTTAAGCAAGCGAGTACTTTGTTGGCTTTGTTCGCCGGCGGGGATGGGTATAGGGTGGAGGAGAATAACGGGTGTCTTATGCTTGGGTGGCACACGAGGTCACTCATTGCTACCTCCGCGTGGAAGCTTCCACTCAGTGAGTCAAAGTGAGTTGACTCTGCGGTGAATAAGTGCGAGTTGAAATTAATTGAATTGGATGGGTTTTTAATTATGAAAACATGACCAATTAGGGTGGCAGATTGGCTGAAACTTAATCTTTGATGTTGATGACTGAAAAGTGACTCAGTGAGTGAGCGAGCGAGCGAGGCAGGGAGAAATAAAGAGAAGTAAAAAAAAAAAGTAAAAACAATGAAAGTTCTGTCTGTAATTTGTAATTGTAATCTTAATTTCGANTATTATTAGTNNTTTTTNNTGCTTCTTAAAATTTAAATTAATTTTTTTAAATYATGAGCTAG
TARGET=285,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04419 Ps_contig_mira-and-tgicl-ass_franssen_33180
SEQUENCE=ACTCTCTTCTATCTCCGTTTCCCCTTTCTCCTCTCTCAAACCCTTTTTTCCATTTTTGAAATTCAACCCATTTCACTTCCATGATATCACCCAATTCAATTTATAAATTCTAATCTTCTAATCTTCTAATCTTTTAATCTAGTCATTTCACAAATTTCCCCAACAAGATGAAGAGAGATCGTCAAGAAACCTCTGGTGGAGGTGTGAGTAGCAACAATAACAATAGCGACAACATCATCAGCAACAACTTTGTTAGTGTACGAGGTGAATGTTCATCAATGCAGAACAACAACAACAACGGAAAACAAAGTTTGTGGAGCGAAGAAAAAGAGAACAGTGGTGGAATGGACGAGTTACTAGCGGCATTAGGTTACAAGGTTCGTTCTTCAGACATGCTTGACGTAGCTCAAAAGCTTGAACAACTTGAGATGGTGATGGGTAGTGCTCAAGAGGAAGGAATTAACCATCTTGCTTCAGATACAGTTCACTACGATCCCACCGATCTATATTCATGGGTTCAAACCATGCTCACTGAACTTAACCCTTCCACCGATTCACAAATCAATGACCCTTTAGATTCTTCTTCTTCCATCTTCAACGATAACTCGCAGTATGATCTCAGTGTTATTCCTGGAATGGCCGCATACCCACCTCAGAGCCATAACCAAAACAGCGAAAGTGAAGGTTCTAACAGCAACAAAAGGTTGAAGACGTGGGGGAGTGAAACGGAATCGGAAGATATTTTTCTGCCGGCGTTATCACCGCCGGCGGAGACTACAAGACCGGTGGTTCTCGTTGACTCACAGGAAACTGGGGTTCGTCTTATTCACACTATGATGGCTTGCGCCGATGCGATTCAGCGTGATGATATCAAGATTGCTGATAGACTCGTGAAGAATATTGGAATATTGGCTTCGTCGCAAACCGGAGCGATGGGAAAAGTTGCTTCGTATTTCGCACAAGCTCTGTATCGGAGAATCTGCAGAGTTTCCCCTGATGAAACTCTGGATTCATCTTTGTCTGATGCACTTCACATGCACTTTTACGAGTCTTCTCCTTATCTCAAATTCGCTCACTTCACCGCTAATCAAGCTATTCTGGAAGCTTTTGCCGGTGCCGGAAGTGTTCACGTCATTGATTTTGGTCTCAAACAAGGGATGCAGTGGCCGGCGCTTATGCAAGCACTTGCATTACGTCCTGGTGGTCCACCAACTTTCCGGTTAACCGGAATCGGACCACCGCAGACGGGTAACACCGATGCTTTGCAGCAGGTGGGTTGGAAATTGGCTCAGCTTGCTCAGACCATTGGTGTTCAGTTCGAATTTCGTGGATTCGTTTGCAACAGTCTCGCGGATCTTGACCCGAATATGCTCGAGATCCGACCCGGTGAAGCCGTTGCTGTTAACTCCGTTTTCGAACTTCACACCATGTTAGCCCGACCCGGTTCCATTGATAAGGTTCTCAACACTGTTAAGAAGATTAACCCTAAGATTGTCACTATCGTGGAGCAAGAAGCGAATCATAACGGACCGGTTTTCATGGATCGGTTCACGGAGGCTTTGCATTATTACTCGAGTTTATTTGATTCTCTGGAGGGTTCTTCTAATTCAAACCCGGCCGGGTCTGGTTCTTCGAGTCAGGATCTGTTGATGTCTGAGCTTTACTTGGGGAGACAGATATGCAACGTGGTGGCTTATGAAGGTGTGGACCGGGTCGAGAGACACGAGACACTGAGTCAGTGGAGGTCGAGGATGGGTTCAGCTGGGTTCGACCCGGTTCATTTAGGTTCAAACGCGTTTAAGCAAGCGAGTACTTTGTTGGCTTTGTTCGCCGGCGGGGATGGGTATAGGGTGGAGGAGAATAACGGGTGTCTTATGCTTGGGTGGCACACGAGGTCACTCATTGCTACCTCCGCGTGGAAGCTTCCACTCAGTGAGTCAAAGTGAGTTGACTCTGCGGTGAATAAGTGCGAGTTGAAATTAATTGAATTGGATGGGTTTTTAATTATGAAAACATGACCAATTAGGGTGGCAGATTGGCTGAAACTTAATCTTTGATGTTGATGACTGAAAAGTGACTCAGTGAGTGAGCGAGCGAGCGAGGCAGGGAGAAATAAAGAGAAGTAAAAAAAAAAAGTAAAAACAATGAAAGTTCTGTCTGTAATTTGTAATTGTAATCTTAATTTCGANTATTATTAGTNNTTTTTNNTGCTTCTTAAAATTTAAATTAATTTTTTTAAATYATGAGCTAG
TARGET=578,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04420 Ps_contig_mira-and-tgicl-ass_franssen_33180
SEQUENCE=ACTCTCTTCTATCTCCGTTTCCCCTTTCTCCTCTCTCAAACCCTTTTTTCCATTTTTGAAATTCAACCCATTTCACTTCCATGATATCACCCAATTCAATTTATAAATTCTAATCTTCTAATCTTCTAATCTTTTAATCTAGTCATTTCACAAATTTCCCCAACAAGATGAAGAGAGATCGTCAAGAAACCTCTGGTGGAGGTGTGAGTAGCAACAATAACAATAGCGACAACATCATCAGCAACAACTTTGTTAGTGTACGAGGTGAATGTTCATCAATGCAGAACAACAACAACAACGGAAAACAAAGTTTGTGGAGCGAAGAAAAAGAGAACAGTGGTGGAATGGACGAGTTACTAGCGGCATTAGGTTACAAGGTTCGTTCTTCAGACATGCTTGACGTAGCTCAAAAGCTTGAACAACTTGAGATGGTGATGGGTAGTGCTCAAGAGGAAGGAATTAACCATCTTGCTTCAGATACAGTTCACTACGATCCCACCGATCTATATTCATGGGTTCAAACCATGCTCACTGAACTTAACCCTTCCACCGATTCACAAATCAATGACCCTTTAGATTCTTCTTCTTCCATCTTCAACGATAACTCGCAGTATGATCTCAGTGTTATTCCTGGAATGGCCGCATACCCACCTCAGAGCCATAACCAAAACAGCGAAAGTGAAGGTTCTAACAGCAACAAAAGGTTGAAGACGTGGGGGAGTGAAACGGAATCGGAAGATATTTTTCTGCCGGCGTTATCACCGCCGGCGGAGACTACAAGACCGGTGGTTCTCGTTGACTCACAGGAAACTGGGGTTCGTCTTATTCACACTATGATGGCTTGCGCCGATGCGATTCAGCGTGATGATATCAAGATTGCTGATAGACTCGTGAAGAATATTGGAATATTGGCTTCGTCGCAAACCGGAGCGATGGGAAAAGTTGCTTCGTATTTCGCACAAGCTCTGTATCGGAGAATCTGCAGAGTTTCCCCTGATGAAACTCTGGATTCATCTTTGTCTGATGCACTTCACATGCACTTTTACGAGTCTTCTCCTTATCTCAAATTCGCTCACTTCACCGCTAATCAAGCTATTCTGGAAGCTTTTGCCGGTGCCGGAAGTGTTCACGTCATTGATTTTGGTCTCAAACAAGGGATGCAGTGGCCGGCGCTTATGCAAGCACTTGCATTACGTCCTGGTGGTCCACCAACTTTCCGGTTAACCGGAATCGGACCACCGCAGACGGGTAACACCGATGCTTTGCAGCAGGTGGGTTGGAAATTGGCTCAGCTTGCTCAGACCATTGGTGTTCAGTTCGAATTTCGTGGATTCGTTTGCAACAGTCTCGCGGATCTTGACCCGAATATGCTCGAGATCCGACCCGGTGAAGCCGTTGCTGTTAACTCCGTTTTCGAACTTCACACCATGTTAGCCCGACCCGGTTCCATTGATAAGGTTCTCAACACTGTTAAGAAGATTAACCCTAAGATTGTCACTATCGTGGAGCAAGAAGCGAATCATAACGGACCGGTTTTCATGGATCGGTTCACGGAGGCTTTGCATTATTACTCGAGTTTATTTGATTCTCTGGAGGGTTCTTCTAATTCAAACCCGGCCGGGTCTGGTTCTTCGAGTCAGGATCTGTTGATGTCTGAGCTTTACTTGGGGAGACAGATATGCAACGTGGTGGCTTATGAAGGTGTGGACCGGGTCGAGAGACACGAGACACTGAGTCAGTGGAGGTCGAGGATGGGTTCAGCTGGGTTCGACCCGGTTCATTTAGGTTCAAACGCGTTTAAGCAAGCGAGTACTTTGTTGGCTTTGTTCGCCGGCGGGGATGGGTATAGGGTGGAGGAGAATAACGGGTGTCTTATGCTTGGGTGGCACACGAGGTCACTCATTGCTACCTCCGCGTGGAAGCTTCCACTCAGTGAGTCAAAGTGAGTTGACTCTGCGGTGAATAAGTGCGAGTTGAAATTAATTGAATTGGATGGGTTTTTAATTATGAAAACATGACCAATTAGGGTGGCAGATTGGCTGAAACTTAATCTTTGATGTTGATGACTGAAAAGTGACTCAGTGAGTGAGCGAGCGAGCGAGGCAGGGAGAAATAAAGAGAAGTAAAAAAAAAAAGTAAAAACAATGAAAGTTCTGTCTGTAATTTGTAATTGTAATCTTAATTTCGANTATTATTAGTNNTTTTTNNTGCTTCTTAAAATTTAAATTAATTTTTTTAAATYATGAGCTAG
TARGET=2088,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04421 Ps_contig_mira-and-tgicl-ass_franssen_33181
SEQUENCE=GTGTGATCTTCAGGATCAGTCAATGGCATTTGGCTCTGATCGTGGTCGGTTCACTGATATGAAGAGATCTGTGGTGGACAAAAATCTTGGACCATTGGTGAAGACTGTGATGACTCGGTGCATTCAATGTACAAGGTGTGTTAGATTTGCATCAGAGGTTGCAGGAGTTCAGGATCTTGGCATGTTAGGTCGTGGCAGCGGAGAAGAGATTGGAACATATGTTGAAAAACTCATGACAAGTGAACTATCTGGAAATGTGATAGATATCTGTCCTGTAGGAGCACTCACTTCAAAACCTTTTGCATTTAAAGCCCGCAATTGGGAGTTGAAGGGTACAGAGACCATTGATGTTACTGACGCTGTTGGGTCCAATATTCGAATTGATAGCAGAGGACCTGAAGTCTTGCGTATTGTTCCTCGTTTAAATGAGGACATAAATGAAGAATGGATTTCAGACAAAACCCGCTTTTGTTATGATGGTTTGAAGAGGCAGAGGTTAAATGACCCTATGATTCGTGGTCCTGATGGTCGCTTTAAGCCTGTGAACTGGGGTGATGCCCTAGCTGTGGTAGCAGAGGCTGCACATCAAGTTAAACCAGATGAAATTGTTGGAATATCTGGCAAGCTTTCTGATGCTGAGTCCATGATTGTTCTAAAAGATTTCTTAAACAGGATGGGGTCAAATAATGTGTGGGGTGAAGGCATTGGCGAAAATACTAATGCTGATTTGAGATCAGGATATATCATGAATACTAGCATTGCTGGTCTAGAGAAAGCAGATGCTTTCCTATTGGTTGGCACCCAGCCACGGGTGGAAGCTGCTATGGTTAATGCCAGAATACGCAAGACTGTCGTATCAAACCACGCCAAAGTAGGTTACGTTGGGCCTGCTACTGATTTCAACTATGATCACCAACATCTTGGTACTGGTCCACAAACACTTGTTGAAATTGCTGAGGGTCGACACCCATTTTCCAAGACTATTTCAAATGCCAAGCATCCTGTTATCATTGTCGGTGCTGGGATTTTTGAGAGGAAGGATCAGGATGCAATTTTTGGCGCTATTGAAACCATTGCAAAACAAGGGAATGTTGTCAGGTCTGATTGGAATGGCCTTAATGTATTACTTCTCCATGCTGCCCAGGCGGCTGCACTTGACCTTGGACTTGTGCCCCAATCTGAAAAAAGCCTTGAATCTGCAAAGTTTGTATATTTGATGGGAGCTGATGATACCAACATAGACAAGATCCCAAAGGATGCTTTTGTTGTTTATCAAGGTCACCATGGTGATAAGAGTGTTTATCGTGCCGACGTCATTTTGCCAGCAGCAGCATTCAGTGAGAAGGAAGGAACCTATGAAAATACTGAAGGATGCACACAACAAACATGGCCTGCAGTTCCAACAGTTGGTGACTCCAGAGATGATTGGAAGATAATTCGAGCTTTATCTGAGGTAGCAGGGGTGCGCTTACCCTACGATACAATTGGGGGTGTCCGCTCCCGATTGAGGACTGTAGCACCAAACCTTGCGCAAATGGATGAGAGAACACCAACTGCTTTGCCATCTTCACTGAGACCAACCTTCACTCAGAAGGTGGATCCAACTCCATTTGGGGTTGCAGTTGAGAATTTCTATATGACTGATGCCATTACCAGGGCATCAAAGATAATGGCTCAATGCAGTGCTATGCTGTTGAAGAAATGAAGCACGTATTATTTATTTATTTTTGGTTCAAATAAAAATGCAGATTCATGCTTTTCTTTTTGTGGGCAATCAAGACCTTGCTTGCGCAAAACTTCGGGAAGTTGGAAATACCCTATATTTTGAAATTAGGCTTTATTCGTTCCATTTGTATTATGCTGGTGTAAAAGCCACTACTTTTTTGAACTGTTGCCATGTACAATTTGGGCTACCTGACTTGATTATTATTTTACTCGTCTCATAAAATGAAACCTTGTTGGAAGCRTAAANRNTTCCAAACC
TARGET=1714,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04422 Ps_contig_mira-and-tgicl-ass_franssen_33182
SEQUENCE=TTTTAAACTTTTTACTTAACTACTTCCTTCGTACATCAACACAAATAACATCCACTCTTCACATTTAGTAGTTTCTATAAATTTTAAAAACTAAAAAAATTTAAGTAATATTTTCTATTCATGCTCAATCAATCAACCAAATCAAATCATCATAAAACTAAAACAAACATCCTAGCCGACCCAACAAAAGGGGAGGGGGTGTTGCCAACAACATTCATCCAATCAATGTTTCTTGAGAAAAGTCTTTAAAGAAAAAAACAATGCAATTTCTTTGAGGAGGTGAATCTATTATAGATGAAATTTATAATCAAATATTGTTGTTTTAATCATCATCATGCAAGACACAATCATCAAGTATTTGTCTCTGTACAAAGTCTTCACTTTTGTTTCGGAGTTGCATTCCGCTTCGACTCAAGTGTTGTTCCTTCTTTAAGTGCTGCTTGTGCTTCATTCTCCATTCCAACTGTTGCAAGTGAAACTGATTGAAGATAAGATGCAATGTGCCATATTGGTGAAACAACCTGCGCTTGCATTGCGTCGTTCAGCGCTTCCTGAGCCATGTCGCTTATGAGATAACACAAACTACGTCGTGCATAGACTGTTGGAGAAACCATTGTTCCAGCATCGATGAACTGTGTGTAGCACTCGATCGCTTCTCTGAATTCTTTCTGTCGGAAAGCGGCATCGCCCTTTTTCTTGCAATTCAATGTGTCCTGCATTTGATCAGTCCACATCTGGAATGATAACTCATTCGCCACTCCTTCATCATCTTTATAACCAAGATTATCCAGAACTTCGTGTATGGCGGTCAAGTCCTTTCTCGAACAAGCCTCGCCAAGTGGAGATAATGAAGCAAAACTTGTGCTATGTGGTATACCCATCAACACATGTGAAGGAACCTCGGTTTCTTTCTGAAGAGAAGCCAAAGCAGACACCAATGATTTTGGATTAGGCCTTTCTCTAGGTTCATATTGCAAACATCGAGATGCCAAGCGTACAAGCTCAGTTCCATCGTTATCAGAAAATTGTCCTTCCAGACAAGAATCTGTCAGCATCTGAAGATTTCTGTCCCGGATCAAATCAAGAGCATGACTCGGCGGGATATGCTTTCCGCTAAGAAGGTCAAGCAAAAGAGTGCCAAAGCTATATATTACGCTTTCTGGTGTTACTCTCCCAGTTCTGAGATACTCTGGAGGAGTAAATGCCAAATTGGTGCTATAACTTTTCCCGTCCCTGCTATTTTTCATAAGTCCAAAAGTAGATAGCCTAGGATTACCGTCCTCGTCAAAGAGAACTCTATATGCGTTGAGGTCGTGATAGAGAGCACGTCCTTTACTTGTGCAGTACTCTAGAGCTTGTGCAAGATGTAGAACAACCCTTAGTCGACAATTACGACCCCCCGAC
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.3786
PRIMER_LEFT_PENALTY=2.186162
PRIMER_RIGHT_PENALTY=1.192395
PRIMER_LEFT_SEQUENCE=CTTCCTTCGTACATCAACACAA
PRIMER_RIGHT_SEQUENCE=GATTGGATGAATGTTGTTGGC
PRIMER_LEFT=22,22
PRIMER_RIGHT=223,21
PRIMER_LEFT_TM=57.814
PRIMER_RIGHT_TM=60.192
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=3.9261
PRIMER_LEFT_1_PENALTY=2.733732
PRIMER_RIGHT_1_PENALTY=1.192395
PRIMER_LEFT_1_SEQUENCE=ACTTCCTTCGTACATCAACACA
PRIMER_RIGHT_1_SEQUENCE=GATTGGATGAATGTTGTTGGC
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=223,21
PRIMER_LEFT_1_TM=57.266
PRIMER_RIGHT_1_TM=60.192
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=4.1002
PRIMER_LEFT_2_PENALTY=2.733732
PRIMER_RIGHT_2_PENALTY=1.366457
PRIMER_LEFT_2_SEQUENCE=ACTTCCTTCGTACATCAACACA
PRIMER_RIGHT_2_SEQUENCE=GGTCGGCTAGGATGTTTGTTT
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=180,21
PRIMER_LEFT_2_TM=57.266
PRIMER_RIGHT_2_TM=60.366
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=4.5458
PRIMER_LEFT_3_PENALTY=3.353417
PRIMER_RIGHT_3_PENALTY=1.192395
PRIMER_LEFT_3_SEQUENCE=CTACTTCCTTCGTACATCAACACAA
PRIMER_RIGHT_3_SEQUENCE=GATTGGATGAATGTTGTTGGC
PRIMER_LEFT_3=19,25
PRIMER_RIGHT_3=223,21
PRIMER_LEFT_3_TM=59.647
PRIMER_RIGHT_3_TM=60.192
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=4.7697
PRIMER_LEFT_4_PENALTY=2.733732
PRIMER_RIGHT_4_PENALTY=2.035956
PRIMER_LEFT_4_SEQUENCE=ACTTCCTTCGTACATCAACACA
PRIMER_RIGHT_4_SEQUENCE=GTCGGCTAGGATGTTTGTTTTAGT
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=179,24
PRIMER_LEFT_4_TM=57.266
PRIMER_RIGHT_4_TM=59.964
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_04423 Ps_contig_mira-and-tgicl-ass_franssen_33189
SEQUENCE=AGCAAATGGAAGATTTACATGAATTTGTCACTTTATGTGACTTCAACATCGATGGCACTTGGTAGCAATAGTTAATAGATTGATAATTTTGTTTGTGCTTTTACACTTGGAGGAGGGCATGTACATATACATATTATACAACAGATTGTCCTCTTTGTTCTATGAGCTACACAGAGCATGCATTTTGAGGCTTACATAAATAATCTAGTTGTGAAACTTGTTACACCCAAATTTAAGAGCATAGGTGATCACTCAAATAACCTAGTTGTGAAACTTGGTAGAAGCAAAGAGAACAACGAAAGAACAGGAAACAGAAGATAAGCCAGTTAAACTCATAGGTGATCTCGCGCCAGCCTAACTGGTTTTTCCTTTAAAAATGCAACATCGCCATTCGATGTACTTAGCAGGCGGTGATTTGGCTCCTCCTCCTCTTCACTCTCGATGGACAGAGAAAGGTCGTCAGAATCTGATCTATCATCATCAGGAAATGAAAGACGTTCGGGGACATGTCCAACTGCAGAAACCTGGATGTTTTCTGGTAGTAGACAATTGCAAAAGGAACCTACACGAGCAAGTCGATTAACCCATCCAGGAATAGGATTTCCAGTTAGTTGTTGGCAAACTTCATCCGTAAAATGGTTGCAATTTTTGGCAATCAGATGGTAAGTGTCTCCATGATATTTTGCAGAGAGACGCTCCATAAAAGAACGGAATTCTGCAAGAGACATATCAGTGCTTCCCAACAATAGCGATCGTCTGAAGATGAAGCCAGGGCAATTTTTAGGTTCCACCTGAAACACACCACTTGAAGGGTATTCGTGTGCTCCAAAGCCGTATTCCATACCATGCACTTCAATACCGGAGTGAAATATTCCAAGGCCAAATAGGTAAAGATAATTATTGATAGGAGTAAGATCATATACATTGAGATACACCATAGCACGAGATTTATTCCCACTGTTCCGGTCCCTTTCCGAGCTACTACTCAGTTGAAACCACCGCATTATGGTGGAAAGTTTCTGTCCAATTATGAATCACAGCCTCATCAACTTCACTCCCAATGCAGAAATGAGAGAGAGAGAGAGAGAGAGAATTCAACGAGAATTAAATTAGAATTGAAATTACAGTGAGTTTGGATTTGGATTGGGATTTGAGTTGTGTTCTTTCGCCGTCATCAAGATTTACCAGCAAAGGCATATGTAACAACTTTTTAGTTTNCCCCGA
TARGET=420,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0482
PRIMER_LEFT_PENALTY=0.048131
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=ATGTACTTAGCAGGCGGTGATT
PRIMER_RIGHT_SEQUENCE=GACACTTACCATCTGATTGCCA
PRIMER_LEFT=394,22
PRIMER_RIGHT=670,22
PRIMER_LEFT_TM=60.048
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=277
PRIMER_PAIR_PENALTY_1=0.0517
PRIMER_LEFT_1_PENALTY=0.048131
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=ATGTACTTAGCAGGCGGTGATT
PRIMER_RIGHT_1_SEQUENCE=CACTTACCATCTGATTGCCAAA
PRIMER_LEFT_1=394,22
PRIMER_RIGHT_1=668,22
PRIMER_LEFT_1_TM=60.048
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.2286
PRIMER_LEFT_2_PENALTY=0.048131
PRIMER_RIGHT_2_PENALTY=0.180440
PRIMER_LEFT_2_SEQUENCE=ATGTACTTAGCAGGCGGTGATT
PRIMER_RIGHT_2_SEQUENCE=CTTGCTCGTGTAGGTTCCTTTT
PRIMER_LEFT_2=394,22
PRIMER_RIGHT_2=574,22
PRIMER_LEFT_2_TM=60.048
PRIMER_RIGHT_2_TM=59.820
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=181
PRIMER_PAIR_PENALTY_3=0.2317
PRIMER_LEFT_3_PENALTY=0.048131
PRIMER_RIGHT_3_PENALTY=0.183585
PRIMER_LEFT_3_SEQUENCE=ATGTACTTAGCAGGCGGTGATT
PRIMER_RIGHT_3_SEQUENCE=GACTTGCTCGTGTAGGTTCCTT
PRIMER_LEFT_3=394,22
PRIMER_RIGHT_3=576,22
PRIMER_LEFT_3_TM=60.048
PRIMER_RIGHT_3_TM=59.816
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.2404
PRIMER_LEFT_4_PENALTY=0.048131
PRIMER_RIGHT_4_PENALTY=0.192310
PRIMER_LEFT_4_SEQUENCE=ATGTACTTAGCAGGCGGTGATT
PRIMER_RIGHT_4_SEQUENCE=AATCGACTTGCTCGTGTAGGTT
PRIMER_LEFT_4=394,22
PRIMER_RIGHT_4=580,22
PRIMER_LEFT_4_TM=60.048
PRIMER_RIGHT_4_TM=60.192
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_04424 Ps_contig_mira-and-tgicl-ass_franssen_33189
SEQUENCE=AGCAAATGGAAGATTTACATGAATTTGTCACTTTATGTGACTTCAACATCGATGGCACTTGGTAGCAATAGTTAATAGATTGATAATTTTGTTTGTGCTTTTACACTTGGAGGAGGGCATGTACATATACATATTATACAACAGATTGTCCTCTTTGTTCTATGAGCTACACAGAGCATGCATTTTGAGGCTTACATAAATAATCTAGTTGTGAAACTTGTTACACCCAAATTTAAGAGCATAGGTGATCACTCAAATAACCTAGTTGTGAAACTTGGTAGAAGCAAAGAGAACAACGAAAGAACAGGAAACAGAAGATAAGCCAGTTAAACTCATAGGTGATCTCGCGCCAGCCTAACTGGTTTTTCCTTTAAAAATGCAACATCGCCATTCGATGTACTTAGCAGGCGGTGATTTGGCTCCTCCTCCTCTTCACTCTCGATGGACAGAGAAAGGTCGTCAGAATCTGATCTATCATCATCAGGAAATGAAAGACGTTCGGGGACATGTCCAACTGCAGAAACCTGGATGTTTTCTGGTAGTAGACAATTGCAAAAGGAACCTACACGAGCAAGTCGATTAACCCATCCAGGAATAGGATTTCCAGTTAGTTGTTGGCAAACTTCATCCGTAAAATGGTTGCAATTTTTGGCAATCAGATGGTAAGTGTCTCCATGATATTTTGCAGAGAGACGCTCCATAAAAGAACGGAATTCTGCAAGAGACATATCAGTGCTTCCCAACAATAGCGATCGTCTGAAGATGAAGCCAGGGCAATTTTTAGGTTCCACCTGAAACACACCACTTGAAGGGTATTCGTGTGCTCCAAAGCCGTATTCCATACCATGCACTTCAATACCGGAGTGAAATATTCCAAGGCCAAATAGGTAAAGATAATTATTGATAGGAGTAAGATCATATACATTGAGATACACCATAGCACGAGATTTATTCCCACTGTTCCGGTCCCTTTCCGAGCTACTACTCAGTTGAAACCACCGCATTATGGTGGAAAGTTTCTGTCCAATTATGAATCACAGCCTCATCAACTTCACTCCCAATGCAGAAATGAGAGAGAGAGAGAGAGAGAGAATTCAACGAGAATTAAATTAGAATTGAAATTACAGTGAGTTTGGATTTGGATTGGGATTTGAGTTGTGTTCTTTCGCCGTCATCAAGATTTACCAGCAAAGGCATATGTAACAACTTTTTAGTTTNCCCCGA
TARGET=1071,22
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0246
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.020269
PRIMER_LEFT_SEQUENCE=ACTTCACTCCCAATGCAGAAAT
PRIMER_RIGHT_SEQUENCE=TTGTTACATATGCCTTTGCTGG
PRIMER_LEFT=1048,22
PRIMER_RIGHT=1205,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.020
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=0.0604
PRIMER_LEFT_1_PENALTY=0.040146
PRIMER_RIGHT_1_PENALTY=0.020269
PRIMER_LEFT_1_SEQUENCE=CCAATTATGAATCACAGCCTCA
PRIMER_RIGHT_1_SEQUENCE=TTGTTACATATGCCTTTGCTGG
PRIMER_LEFT_1=1023,22
PRIMER_RIGHT_1=1205,22
PRIMER_LEFT_1_TM=59.960
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.0999
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=ACTTCACTCCCAATGCAGAAAT
PRIMER_RIGHT_2_SEQUENCE=AACACAACTCAAATCCCAATCC
PRIMER_LEFT_2=1048,22
PRIMER_RIGHT_2=1161,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=0.1062
PRIMER_LEFT_3_PENALTY=0.085951
PRIMER_RIGHT_3_PENALTY=0.020269
PRIMER_LEFT_3_SEQUENCE=CACAGCCTCATCAACTTCACTC
PRIMER_RIGHT_3_SEQUENCE=TTGTTACATATGCCTTTGCTGG
PRIMER_LEFT_3=1035,22
PRIMER_RIGHT_3=1205,22
PRIMER_LEFT_3_TM=59.914
PRIMER_RIGHT_3_TM=60.020
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.1062
PRIMER_LEFT_4_PENALTY=0.085951
PRIMER_RIGHT_4_PENALTY=0.020269
PRIMER_LEFT_4_SEQUENCE=TCACAGCCTCATCAACTTCACT
PRIMER_RIGHT_4_SEQUENCE=TTGTTACATATGCCTTTGCTGG
PRIMER_LEFT_4=1034,22
PRIMER_RIGHT_4=1205,22
PRIMER_LEFT_4_TM=59.914
PRIMER_RIGHT_4_TM=60.020
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_04425 Ps_contig_mira-and-tgicl-ass_franssen_33191
SEQUENCE=AATAAAAGATTGATTTCATATATTTATTTCATTATTGAAAAATGATAATGAAAAAGTGTTGCAAAATTACATGATGTTGCGTGTATATATATACATGTCAATTTAAATAACTAATGCATTGAAACAAAATATACTGCGTGCAATGACTACAAAAAATGGTCACATATCGACTGCAGTATTAGCAAATGCTATGCTTTTAGGGAGAGTAAAAGATGCACACAGCATCCATTTTCCAGGTGCAACCAGTCGTACTCGTCGCATTTCGACATTGACTGCCACTTCAAGCAATGCAATTCTTATTCTCTCATGAAAATCAAATTCTGGATCCTCTGCTTTGGAGAATCCATAAACATGGATCATTGGATAAGTAAATTGCCCATCCTTGGATCTATCTTTGTATATTCCCCGAAATGCATCTAGAAATTCTGCAGCGTCATTCGGCAAGTTCATAACTACTTGTGTAATGGAGTGAGCTTTATTACTGGCAAACATAGCTTTAATAAACCTTCTTCCATCCATGTTAAAGACCTTAATTTTCTTCTCAAGTTTGTTTAGAACACTATTTCTTTCCAGGTACTCGACTGCATATGGGTTCAAGTCATTAGCAAAAACACGCTTAACTATCTTTGCTGCAGAGATGGCAAGGGGCCCAACTCCAGAAAAAACATCACAGACAACATCATCTCGTGTAAAACCACTCAATAGTCTCTGTCTTTCGGTGCCCAGCCTACTGCTCCAGTATACTGTTGCTAAGTCAACATGAAAGCGAAGTCCGTTCTCAGCTAATGTAGTCACAAGAGAGTGGTTTCCTGCTAAAACCTCCAGCTGCATGGTCCTGTACTCATTATGAATGGAGTCAATCTTGTTCGCAACTGTTTGTATCTTTGGTTTATTTTTATCCAGCACAACCTTTGCTATAAGCTTCTTGTATGGTAAATGTTCCTCTCTCAAATTCAGGTGCGCAATATGACCAACAGTTTCAAAAGCTGTAGGAACAATCATACCCTCGGGCAGCAAGACCTCCAAGATCTCATTCATCTGCCAGTAATCATAAAACAGAGTCAACTTGCATCTAACTAGCTCAAAAGTCAAAGTGGTGCTTTTTTCTGCATACTCCTTCAATACAGCCTTTATCGCTTCAGGCAACTCCTCCATACGAAGATCCGCATAGTTTTCATCAAGAAGCAACAGCCTCGTGGAACCCCTCCACTTGCTCCTCCCAAACTCTTCACCAAGTAACCTCCTCAAACCCTCATCAGCATCTCCTCCTTCTTCAACAACCTCAATCGCAACAAAATCTTTCCTCCCAACACGCTTACTCCCTTTAGCCTCGACCAATTTCCCCACCTCCCCATCCTTCTTCTTCCTATTCCTACTCGGCCTCGAAATCTTCGCCAGGTTCCGAAACTTAACATATCCGCGAGTGTTAAACGTTTTAGCAAGCTTATCACGGTACAACAC
TARGET=84,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04426 Ps_contig_mira-and-tgicl-ass_franssen_33192
SEQUENCE=AAAATAGTAGCCAAGAGCAAGTTATGTACCAGGTGATACGAGTCTATAAAATGTTATACTCAGCAATATGCATTCAAAACCTTTGTTTATAGTACTTCACTACTACTATTCCCCAACTGCACCCTTCCCTTCCCCAACAGCAAGATGACTCGCTCAAATTTCAATGACAAATACATGAATAGAATGTAAATAACTCCCAGACCCTGAAAGCGCGAATCATTAGATTCGGGGATTCAAACCTGACTCAAAACCCACTTTGCTGACAACAACTCAACAAAGTATATGGACCACTCAATAACGTAGCAAAGCAACAAAACAATATGAAGACTGACTATGAGATAGCAATGATAAGTGTGGCGGCACATAGGCAAACCACCTAACCTTGTCATATTGCCTTGTCAACCATCATAATCAATCAGATGAACCAGACTCTTTAACTTCCTTCTCTGAATCTCCTTTTTCTTTTTCTGGTGCAGATTCTGGCTCCTTAGTTTTGTCCTCAGGTTTCTCTTCATCTTTCTTCTCTTCATCTTTCTTTTCTGCATCTGATTTCAGATCAACACTCAATTTCACAAGGAGTTCAGCAGCAGCAGTTACTTCCTCGTTTTCCTCTTTCTTCAGGGAATCGGCAATTTCATGGAATGTTTCTATGAAATTTTTGCAATTTTCAATTGAAGCAAATCGAATGCAGAAAAGCTCATCCTTCAATTCACCATCAGCATAGTCCCTTGCATGCCAAACACAAGACTTCTCATTCCCGGCATGCTCTTGCACAGTCATGTTAGGCAAAATGAGGTGATTTGCACAGATCTTGAGAATCTTCGATTGCCTCATGAGAAGCCTCACTTTTCCGGTAACCTTATGCTTGAGAAACTTCACAGTTCCGGCACCTCGTTCCTTCCACTGATTCCCTTCCTTGTCAAAACGGTACAATTTCGCTTTGAGGTCGAGAATAGCTTCTTCCTCTTCTTCTCCGGTGGATACAGCGACCTCTTCGAGTCTGACGATCGGGGCGACCTGAGCTCCGGTATCTTCGTCTTCGCCGGCGGGAACATCTTCCTCTTCGTGGTGATCGGTGCTGGTGGACATCTTAGTTGATTGAAATTGTAGAGAGAGAAAGAGAGAAACTGTAGAGAGAGAAAATGAGAGTGAGAGCACGGGTTAATAGGTTTTAGGTTTATATAAAAGGAGRAAGAAAGAAGGGTTTCCCCGTCGTACTCTGTCG
TARGET=582,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04427 Ps_contig_mira-and-tgicl-ass_franssen_33193
SEQUENCE=CCTACTCTGCCGTTGCTTGTGTGCTTATGAGCTGATTTGGTNNCTCCTACTGCCATAGCAGATAGTGGACTTGAGCTGCTGTCTACAACTTTTATCTTCAGTTTTCTTGTCACCCTGGCACTCTATTTTTCCGGTTGCAAGTCACGGAATAAAGGTTCATCGCTAAAACCTCATATAAGTGGAAACCTGATAGACGATTGGTGGTTTGGAATACAACTAAATCCTCAGTTCATGGGTATTGACCTCAAATTTTTCTTTGTTAGAGCTGGAATGATGGGGTGGCTACTTATCAACCTATCTGTTCTTGCCAAGAGCATTCAAGATGGTACTTTGAGCAAGTCAATGATTCTCTTCCAGCTATTTTGTGCACTATACATCCTGGACTACTTTGTACACGAAGAATATATGACATCCACATGGGACATAATTGCGGAGAGATTGGGCTTCATGTTGGTCTTCGGAGATTTAGTGTGGATTCCTTTTACTTTTAGCATACAGGGTTGGTGGCTTTTGAGGAACAAGGTAGAGTTAACGACGGCCGCGGCTGTAGCTAATTGCTTTGTCTTCCTTATTGGATACATGGTATTTCGCGGAGCAAACAAGCAAAAACACGATTTCAAGAAGAATCCAAAGTCCCCTATATGGGGTAAGCCTCCAAAAGTCGTTGGAGGCAAGTTACTTGCTTCTGGTTATTGGGGTGTTGCCAGACACTGTAATTACCTAGGGGACTTGTTGCTTGCTCTCTCTTTTAGTTTACCTTGTGGTATCAGTTCACCGGTTCCATACTTCTATCCAATTTATCTTCTCATTCTGTTAATCTGGAGAGAGAGAAGAGACGAAGCTCGATGTGCGGAGAAGTATAAAGATATATGGTTAGAGTATCGGAAACTTGTTCCATGGAGAATATTGCCTTATGTATATTAGCTTCACCATTTGCTTTTTCATCTTGTTCATTGTGTTAACCACTTTAATGTAATCACAATGCATATACATTATGCTATGCGTTGCAGAGAAAAAATTGAGATTTTTGTGTAACCATATTATTTGTGCGAAGTTTTGTGGTGAGAAAAATTAATCCAAACATAGTCAATGAAGTTTAACTTAAA
TARGET=822,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2938
PRIMER_LEFT_PENALTY=0.195770
PRIMER_RIGHT_PENALTY=0.098039
PRIMER_LEFT_SEQUENCE=GGACTTGTTGCTTGCTCTCTCT
PRIMER_RIGHT_SEQUENCE=TCTCTGCAACGCATAGCATAAT
PRIMER_LEFT=725,22
PRIMER_RIGHT=1012,22
PRIMER_LEFT_TM=60.196
PRIMER_RIGHT_TM=59.902
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.2949
PRIMER_LEFT_1_PENALTY=0.196811
PRIMER_RIGHT_1_PENALTY=0.098039
PRIMER_LEFT_1_SEQUENCE=CCGGTTCCATACTTCTATCCAA
PRIMER_RIGHT_1_SEQUENCE=TCTCTGCAACGCATAGCATAAT
PRIMER_LEFT_1=774,22
PRIMER_RIGHT_1=1012,22
PRIMER_LEFT_1_TM=60.197
PRIMER_RIGHT_1_TM=59.902
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=5.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.3411
PRIMER_LEFT_2_PENALTY=0.243031
PRIMER_RIGHT_2_PENALTY=0.098039
PRIMER_LEFT_2_SEQUENCE=ATCAGTTCACCGGTTCCATACT
PRIMER_RIGHT_2_SEQUENCE=TCTCTGCAACGCATAGCATAAT
PRIMER_LEFT_2=765,22
PRIMER_RIGHT_2=1012,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=59.902
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=0.3727
PRIMER_LEFT_3_PENALTY=0.274705
PRIMER_RIGHT_3_PENALTY=0.098039
PRIMER_LEFT_3_SEQUENCE=ACCGGTTCCATACTTCTATCCA
PRIMER_RIGHT_3_SEQUENCE=TCTCTGCAACGCATAGCATAAT
PRIMER_LEFT_3=773,22
PRIMER_RIGHT_3=1012,22
PRIMER_LEFT_3_TM=59.725
PRIMER_RIGHT_3_TM=59.902
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=5.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=0.6230
PRIMER_LEFT_4_PENALTY=0.524968
PRIMER_RIGHT_4_PENALTY=0.098039
PRIMER_LEFT_4_SEQUENCE=TACCTAGGGGACTTGTTGCTTG
PRIMER_RIGHT_4_SEQUENCE=TCTCTGCAACGCATAGCATAAT
PRIMER_LEFT_4=717,22
PRIMER_RIGHT_4=1012,22
PRIMER_LEFT_4_TM=60.525
PRIMER_RIGHT_4_TM=59.902
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=5.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_04428 Ps_contig_mira-and-tgicl-ass_franssen_33196
SEQUENCE=CTTTTCCTCAGTCTTTCTTTCTTTCTTCTCCTCTTGCTTCACCTCAACCTTTGGTTGTTCCTCCTATACTCAATGAATAAAAATAATGGCCAAATATAAATCAAGTACACAATGAGAAAAATATGAATAAGAATCTCAGAAAAAGTTACGTTACCTGTTTTTCTTGCTCACCCATTTTGGTGTATGAAGTATCAAATCACAATTCTGATTTTGGAAAAGATATGCTTAAAGCAA
TARGET=12,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04429 Ps_contig_mira-and-tgicl-ass_franssen_33202
SEQUENCE=AGACACTCTCTCTCTCTCTTAACCCTCTTACTTACTTTCTTTCACCTGGCATTTGATTAGATTAGATTAGATTGAAGTTTAGATCTTTTAGTATTATGTGAAGAAAAAAACTGTATTGGGATGTGTGTTTCTAGATTTGGTCTTTGATTCCATTGGTTCTCTCTATTTTTGTTTGTTTTGGTTTTTTCAATAATGGGTTGTTTTTATTATTCCATATTATGTGTTGTTTTGTTTTTATCCATTGGAAATTCTTTGGCTTCTTCAATTGAAGATCAAAAGAGAGATAAGATTAGTTGGTTACCAGGGCAACCAAAGAATGTTGGATTTGAGCAGTATTCAGGTTATGTGACTGTGAATGAACAGAATGGAAGAGCACTGTTTTACTGGTTGACTGAAGCTCCACTGAGCCGTGGACCCAATTTAAGGCCACTGGTTTTATGGCTTAATGGTGGTCCTGGTTGTTCTTCTATTGCTTATGGTGCTTCTGAAGAAATTGGCCCTTTTCATATTAAGTCTGATGGCAAATCACTTTACTTGAATCCTTATGCTTGGAACAATTTGGCAAATATACTTTTTCTTGATTCTCCGGCTGGGGTTGGTTTTTCTTATTGTAATAAAACAGCAGATCTGTACACATTTGGTGACCAGAAAACAGCTGAGGATGCGTATGTTTTTCTTGTTAATTGGTTTGAAAGATTTCCTCAGTATAAACATAGAGATTTCTACATTGCCGGGGAAAGTTATGCAGGTCATTATGTTCCTCAATTGGCTCAACTTGTTTACCAGAGAAATAAGGGAATCAATAATCCAGTTATCAATTTTAAGGGATTTATGGTTGGAAATGGTGTTACCGATGATTATCATGATTATGTTGGAACATTTGAGTACTGGTGGACGCATGGTTTGATTTCCGATTCCACGTATAAAATTCTAAGAATTGCATGTGACTTTGGATCATCTCAGCATCCATCAGTGCAATGTATGCAGGCTCTTAGACTCGCAGTTGCTGAGCAAGGAAACATTGATCCATATAGCATTTATACACCGCCTTGTAACAACACGTCGTCGCTTTCACGCAGCTTGAACGGTCGTTATCCATGGATGTCAAGAGCGTATGATCCGTGTACCGAAAGGTATTCCAATTTGTATTTCAACCGTCCAGATGTTCAGAAGGCACTTCATGCTAATGTAACAGGGATTCCATATACATGGAAAACATGCAGTGATATTGTTGGGAATTTTTGGACCGATTCGCCTTTGTCTATGCTTCCGATATATCACGAACTTATCAATGCTAGTCTACGAATATGGGTATTCAGTGGAGACACTGATGCTGTAGTTCCACTAACCGCCACTCGATATTCTATAGACGCGTTGAAGCTTCCAACCGTCGTAAATTGGTATCCTTGGTATAACAGTGGCAAGGTTGGTGGATGGAGCCAAGTTTATAAAGGGCTCACGTTCGTGACAGTACGAGGCGCTGGGCACGAGGTTCCTCTTCATAAGCCTCGCGAAGCGTTTATTCTTTTTAGATCATTCTTGGAGAACAAGTACATGCCATCATCATCAAGTTAATCAGATGCATTATTTGTTGTTCTTCTTCACTTCAAGTATAGAACCGAAAAAGTACCTTACATTTTTAAGAATAACTTGGATAATCATATATATACATACATGGAACAGCAGGCTGAGACAGAATAAGGAATGCATTCCTCATGAGTTCCATCTTGAATCTCTGCCTGGAAATTAGAGTTGATAARTGCACCAATAGCAATAGTTGCTCTTCTGTTTTTATTTGTTATTTGAAAAATGTGATTATTTTATGGAAGTGTTATTCTTTATATAGTTTTCACCATGCAAGATTAATGTGATATCTTGTGTAAAAATCTCTTCTCATAAATGAAAAGTACAAACTTTACATTNAA
TARGET=6,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04430 Ps_contig_mira-and-tgicl-ass_franssen_33202
SEQUENCE=AGACACTCTCTCTCTCTCTTAACCCTCTTACTTACTTTCTTTCACCTGGCATTTGATTAGATTAGATTAGATTGAAGTTTAGATCTTTTAGTATTATGTGAAGAAAAAAACTGTATTGGGATGTGTGTTTCTAGATTTGGTCTTTGATTCCATTGGTTCTCTCTATTTTTGTTTGTTTTGGTTTTTTCAATAATGGGTTGTTTTTATTATTCCATATTATGTGTTGTTTTGTTTTTATCCATTGGAAATTCTTTGGCTTCTTCAATTGAAGATCAAAAGAGAGATAAGATTAGTTGGTTACCAGGGCAACCAAAGAATGTTGGATTTGAGCAGTATTCAGGTTATGTGACTGTGAATGAACAGAATGGAAGAGCACTGTTTTACTGGTTGACTGAAGCTCCACTGAGCCGTGGACCCAATTTAAGGCCACTGGTTTTATGGCTTAATGGTGGTCCTGGTTGTTCTTCTATTGCTTATGGTGCTTCTGAAGAAATTGGCCCTTTTCATATTAAGTCTGATGGCAAATCACTTTACTTGAATCCTTATGCTTGGAACAATTTGGCAAATATACTTTTTCTTGATTCTCCGGCTGGGGTTGGTTTTTCTTATTGTAATAAAACAGCAGATCTGTACACATTTGGTGACCAGAAAACAGCTGAGGATGCGTATGTTTTTCTTGTTAATTGGTTTGAAAGATTTCCTCAGTATAAACATAGAGATTTCTACATTGCCGGGGAAAGTTATGCAGGTCATTATGTTCCTCAATTGGCTCAACTTGTTTACCAGAGAAATAAGGGAATCAATAATCCAGTTATCAATTTTAAGGGATTTATGGTTGGAAATGGTGTTACCGATGATTATCATGATTATGTTGGAACATTTGAGTACTGGTGGACGCATGGTTTGATTTCCGATTCCACGTATAAAATTCTAAGAATTGCATGTGACTTTGGATCATCTCAGCATCCATCAGTGCAATGTATGCAGGCTCTTAGACTCGCAGTTGCTGAGCAAGGAAACATTGATCCATATAGCATTTATACACCGCCTTGTAACAACACGTCGTCGCTTTCACGCAGCTTGAACGGTCGTTATCCATGGATGTCAAGAGCGTATGATCCGTGTACCGAAAGGTATTCCAATTTGTATTTCAACCGTCCAGATGTTCAGAAGGCACTTCATGCTAATGTAACAGGGATTCCATATACATGGAAAACATGCAGTGATATTGTTGGGAATTTTTGGACCGATTCGCCTTTGTCTATGCTTCCGATATATCACGAACTTATCAATGCTAGTCTACGAATATGGGTATTCAGTGGAGACACTGATGCTGTAGTTCCACTAACCGCCACTCGATATTCTATAGACGCGTTGAAGCTTCCAACCGTCGTAAATTGGTATCCTTGGTATAACAGTGGCAAGGTTGGTGGATGGAGCCAAGTTTATAAAGGGCTCACGTTCGTGACAGTACGAGGCGCTGGGCACGAGGTTCCTCTTCATAAGCCTCGCGAAGCGTTTATTCTTTTTAGATCATTCTTGGAGAACAAGTACATGCCATCATCATCAAGTTAATCAGATGCATTATTTGTTGTTCTTCTTCACTTCAAGTATAGAACCGAAAAAGTACCTTACATTTTTAAGAATAACTTGGATAATCATATATATACATACATGGAACAGCAGGCTGAGACAGAATAAGGAATGCATTCCTCATGAGTTCCATCTTGAATCTCTGCCTGGAAATTAGAGTTGATAARTGCACCAATAGCAATAGTTGCTCTTCTGTTTTTATTTGTTATTTGAAAAATGTGATTATTTTATGGAAGTGTTATTCTTTATATAGTTTTCACCATGCAAGATTAATGTGATATCTTGTGTAAAAATCTCTTCTCATAAATGAAAAGTACAAACTTTACATTNAA
TARGET=55,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04431 Ps_contig_mira-and-tgicl-ass_franssen_33204
SEQUENCE=ACAACATTCATTCATTCACCATGGCAATGTTTTTCGYTTTTYCTTTTGANYTTRTCTCGTTCCAACCTCAAAAGTGATTACYCTCCTGATCTCATCGATAACAATTTGGTTACACCTCTGTATGACTTCGACAATCCGAGCTATCATGCYGAAGAAGAAGGCGAAGAAGATTGTGATCTGCTGGAATTAGCCAGGTTGTTGAAACAAGAGGAGAAGGTGATTCAACCGCACGAGGAGAAGGTCAAGACTGTCCTCCAGTGAGGCGGAAACCGGTGCTAAAAGATGAGAACGTGTGTGGACTGTCGAGATATGAATAGAGCTAGTTCGAGAGATG
TARGET=5,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04432 Ps_contig_mira-and-tgicl-ass_franssen_33205
SEQUENCE=GATGTTGTTAATGACTTAACGTGCAATCAATTTAATAGTACTATTACCAATATATGTTGTTGTTGTTTCATATAAATAAAACTCCTCAACCAAGTAATTTGTCATTGTCTCATAATTCTCTACTTTACTTTTGTTACTACTGTTAAGTTCACAAGAATAAAGAAAGAATCAGATATTTTTAGGAACCAAATCTGAATCACACAGGAAAAAATGGGTGCTCTAGATCACATTTCTGAACTATTCGACTGTTCCAGCGGCAGTTCTCATCACAAAAGACGCAAACAATTACAGACGGTGGAGGTGAAAGTGAAGATGGACTGCGAAGGATGTGAGAGAAAAGTTAGAAGGTCGGTGGAAGGAATGAAAGGGGTGAACCAAGTTGATATAGAACGGAAAGCAAACAAAGTAACGGTTGTTGGTTATGTGGAACCAAACAAAGTGGTGGCGCGTATAGCTCACCGCACTGGGAAGAGAGCTGAGATATGGCCGTATGTTCCATACGACGTCGTTGAACACCCGTACGCGCAAGGTACGTATGATAAGAAAGCACCTGCTGGTTATGTCAGAAACAACTACGATGATAATCAGTATTCTGGATCTCATCTAGCGCGTGCTAGTTCGACTGAGGTTCGATACACCACCGCTTTCAGCGACGAGAACCCCGCCGCATGTGCTGTTATGTGATGCGATGACGTCACTTTCCGTTACCGGCTTTGTTTGTTATACTAGTAGTATTGTAGTGGCTGTGTTGTGTGTTTATTGAGTTTTTGTAAATTTATTCGTATGGCTTTTTGAGAATCAGTTTTTGGAATAATCGATTTTTTCGATTAAAGATGCCTCTACTCTCCTTGTGCTTATCATTAATTCCAAATATTAAAAAAATTTNTTTTTTATTGAAAATT
TARGET=55,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04433 Ps_contig_mira-and-tgicl-ass_franssen_33210
SEQUENCE=CCCGTGCAACAGTTTTACACCGGCGGCCCCTTCGCCGTCTCATCAGACGGCTCCTTCATCGTCTGTGCATGCGGCGATTCCATTAAAATCGTTGATTCCGCCAACGCATCCATCAGGTCCACCCTCGAAGGCGACTCCGAACAAGTTCACCGCTTTGGCTCTTTCCCCCAACGATAAACTTCTCTTCTCTTCTAGCCACAGCCGCCAAATCAGAGTTTGGGACTTATTCACTCTCAAATGCGTGCGCTCGTGGAAGGGTCACGA
TARGET=179,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3650
PRIMER_LEFT_PENALTY=2.093026
PRIMER_RIGHT_PENALTY=0.271999
PRIMER_LEFT_SEQUENCE=TTAAAATCGTTGATTCCGCC
PRIMER_RIGHT_SEQUENCE=GCACGCATTTGAGAGTGAATAA
PRIMER_LEFT=82,20
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.272
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=12.9000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=2.8622
PRIMER_LEFT_1_PENALTY=2.590232
PRIMER_RIGHT_1_PENALTY=0.271999
PRIMER_LEFT_1_SEQUENCE=GTCTCATCAGACGGCTCCTT
PRIMER_RIGHT_1_SEQUENCE=GCACGCATTTGAGAGTGAATAA
PRIMER_LEFT_1=36,20
PRIMER_RIGHT_1=244,22
PRIMER_LEFT_1_TM=59.410
PRIMER_RIGHT_1_TM=60.272
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=2.8837
PRIMER_LEFT_2_PENALTY=2.611691
PRIMER_RIGHT_2_PENALTY=0.271999
PRIMER_LEFT_2_SEQUENCE=CATTAAAATCGTTGATTCCGCC
PRIMER_RIGHT_2_SEQUENCE=GCACGCATTTGAGAGTGAATAA
PRIMER_LEFT_2=80,22
PRIMER_RIGHT_2=244,22
PRIMER_LEFT_2_TM=62.612
PRIMER_RIGHT_2_TM=60.272
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=12.9000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=2.8837
PRIMER_LEFT_3_PENALTY=2.611691
PRIMER_RIGHT_3_PENALTY=0.271999
PRIMER_LEFT_3_SEQUENCE=CCATTAAAATCGTTGATTCCGC
PRIMER_RIGHT_3_SEQUENCE=GCACGCATTTGAGAGTGAATAA
PRIMER_LEFT_3=79,22
PRIMER_RIGHT_3=244,22
PRIMER_LEFT_3_TM=62.612
PRIMER_RIGHT_3_TM=60.272
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=2.8837
PRIMER_LEFT_4_PENALTY=2.611691
PRIMER_RIGHT_4_PENALTY=0.271999
PRIMER_LEFT_4_SEQUENCE=GGCGATTCCATTAAAATCGTTG
PRIMER_RIGHT_4_SEQUENCE=GCACGCATTTGAGAGTGAATAA
PRIMER_LEFT_4=72,22
PRIMER_RIGHT_4=244,22
PRIMER_LEFT_4_TM=62.612
PRIMER_RIGHT_4_TM=60.272
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_04434 Ps_contig_mira-and-tgicl-ass_franssen_33211
SEQUENCE=CTACAAAATAAACTTCTTTAGAAATTAATTTATTCTAAACACCATACTCTAATTACGATGGCTTTGTTACAATAAAGCAGAAAATCAAGAAGTTTCCACTGTTGTACCAACATAATATATATACCATACGTTTTATAAGTTATAACCATTAAACATAAAGATTACTACTCCTATGATAGTTACACGACAACGGATAAAGAGTGACAAGAACCTTAAATATCTCCTCAGAAAACAGACACAAAATCAGTAACACGTTAAACATGTACACGTGCGCACCCCCTTATCCACAAGAGGCAGAAGATACAAGATTGCATTCAATAAGTATCGTCATACATTGTATGGTGACTATCACCCCATTGTGATTTTGTATGATGACTACCTCCCCATTGTGATTTATCAGCCCTATGGATCAGATTCTCAAATGCTTCTGTTCCATTCTCTTTGATGTACTTCTCTGCAGCAGTTATAATGTCATCTGGCTTAGAGAAGACGTCCTTTTTCCTTGGAACATACCAGATGTTAATGGTCTGAGGATTGGCTAAGAAGGCTCTTACATAATGCTCATAAACATAAGCCGCGCCAGTAAAATAAGGAAGGACCAACCAGCAGGTTAGAATCAGCTTTGCATATGGCCATATAGGAATCCATTCAAGGATTTTGGCAAAAGTAAGTTCAAAAAGAGTGATCATGGAATACAGAACCCAATAAGTTAGCCATTGCTGATCATCCACGGGTGACTTGCTCTCAATTGCCCTAACCGAAGCATATAGAGGATAAACAAGACTAACCACAGGCCCAGCAAGAACATCAATGTTTCTGATAAGCACCATAAGAAAATCACCGGCTCCAGATCCGGATCCAGATCCTCCCATCTTCGATCGAACACTCACCGCAACAGAGGAGGAGATTCCTTGGGAAAGAAAGATGAAGTGAGAGAGAGAGTGTGTGAAAAGTGTCAGTTCCCNNGTACTCTGTCGTTGACTACCAC
TARGET=932,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2424
PRIMER_LEFT_PENALTY=0.191680
PRIMER_RIGHT_PENALTY=0.050763
PRIMER_LEFT_SEQUENCE=TGATCATGGAATACAGAACCCA
PRIMER_RIGHT_SEQUENCE=GGGAACTGACACTTTTCACACA
PRIMER_LEFT=682,22
PRIMER_RIGHT=963,22
PRIMER_LEFT_TM=60.192
PRIMER_RIGHT_TM=60.051
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.2616
PRIMER_LEFT_1_PENALTY=0.210886
PRIMER_RIGHT_1_PENALTY=0.050763
PRIMER_LEFT_1_SEQUENCE=ACTTGCTCTCAATTGCCCTAAC
PRIMER_RIGHT_1_SEQUENCE=GGGAACTGACACTTTTCACACA
PRIMER_LEFT_1=736,22
PRIMER_RIGHT_1=963,22
PRIMER_LEFT_1_TM=59.789
PRIMER_RIGHT_1_TM=60.051
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=0.4052
PRIMER_LEFT_2_PENALTY=0.191680
PRIMER_RIGHT_2_PENALTY=0.213506
PRIMER_LEFT_2_SEQUENCE=TGATCATGGAATACAGAACCCA
PRIMER_RIGHT_2_SEQUENCE=NGGGAACTGACACTTTTCACAC
PRIMER_LEFT_2=682,22
PRIMER_RIGHT_2=964,22
PRIMER_LEFT_2_TM=60.192
PRIMER_RIGHT_2_TM=60.214
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.4143
PRIMER_LEFT_3_PENALTY=0.363508
PRIMER_RIGHT_3_PENALTY=0.050763
PRIMER_LEFT_3_SEQUENCE=AAGCACCATAAGAAAATCACCG
PRIMER_RIGHT_3_SEQUENCE=GGGAACTGACACTTTTCACACA
PRIMER_LEFT_3=821,22
PRIMER_RIGHT_3=963,22
PRIMER_LEFT_3_TM=60.364
PRIMER_RIGHT_3_TM=60.051
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=0.4244
PRIMER_LEFT_4_PENALTY=0.210886
PRIMER_RIGHT_4_PENALTY=0.213506
PRIMER_LEFT_4_SEQUENCE=ACTTGCTCTCAATTGCCCTAAC
PRIMER_RIGHT_4_SEQUENCE=NGGGAACTGACACTTTTCACAC
PRIMER_LEFT_4=736,22
PRIMER_RIGHT_4=964,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=60.214
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_04435 Ps_contig_mira-and-tgicl-ass_franssen_33214
SEQUENCE=TAACAACTATATACTCAAGGAAACCCTCCAATAGGTTGGTTNTTCCATTTGAGCTACATCAGTCAACCGTTTTAGTACATCTCTATTGCCATAATGGGTGCAACAGAAAGAGATCCATAGGGGCATGAAAANGYTAGAAGTTTTTAATTTTATTATATGCCTTAGTTTATTATTCAAGTAAAAATAGAGAAGAAAAAACATGAAGGACTATGCCAAAACTCAAGGTAATACAATGCAGTTCATAGTGATTGGCCAACTGCCTTATTAAAAACACGATCAAGGAAAACCCAGTGGGATAAAACCTTGAAAAAGCGTTTGAATACAAGAAATAATCATTTCTTAAAATATGATCCTATAAATTATTTAAACATCACCATCCCACAACTCTTCCAAGGATTTGTAAGGTCCATTTTCATGTATGAACATTTCTGCTTTTAGGGTTCTCATTTGAGTAATCTGATTGATTTTGTCCGACTCTTCCTGGGTCAATTCCCAATCAAATATTTCAATGTTTTGTTTCATCCTCTCCTTATTGAAGCTTTTCACAATGGCAGTTACTCCATGCTGGTATATCCATCTTAGTACTATCTGGGGTACACTCTTGTTTTTAGCCTCAGCAATTTTGTGCAGAATTGGATTATCCATCACTGCAGCTGAACCCCAAGGATCTTTGTATGCTCCTAGTGGCGACCATGCACCAACATGAATTCCTTTCTGCTTGCAGAATTCTCTGAGTTTCCCTTGATTCCATGATGGGTTCATTTCCACCTGATTAACTGCTGGAGTGATTTTGGCATTTTCTAAAAGTATGGAGAGCTTTTTGGCACCAAAGTTGCTAACACCAATAGACTTTGCTAAGCCCAATCTATGAACCTCTTCCATAGCTTCCCATACTCCTTTTATGTCAATGGGAATCATATCCTCACCTGTAAAGTTTAAGCCTACAACATCTTGTTTGAACCTTACTGGCCAGTGAATCAAATAGAGATCCACATACTCCAAGCCCAGCTTTTTGAGGGTGGTTTCGAGAGCTGGGAGAACAAGGTCATGGTGAGCATCAGTACACCATAACTTGGAAGTAATAAACAATTCGTCGCGATTTTTAACAAGGCCTTCTTCTAAAGCCTTTGACACAGCTTGGCCTAGAAGTTCTTCAGTATTATAGAAAGAAGCCGTATCAAAATGTCTGTAGCCGATTTCAATGGCATCAATCAAAGCTGTTAGAGGAGGAGGAGTTCCTGTTCCATATCCTATCGCCGGCATCTTTTCTCCTGAATTTAAAACCACATCTGGAACACTATTTGCTGCCATCTTCTTTTGTTTTTGTGTTGGTTTAAGGCTCACAATTTTCTGTTATGAATCTTNTACTCTGCGTT
TARGET=1224,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04436 Ps_contig_mira-and-tgicl-ass_franssen_33215
SEQUENCE=AAAACAACGTTCGTTGGCAAAACAAAGAGAAAGAAACTTTTTCAAAGGGGAGGAGAAGAGAAAGAGAAAGAGAAACAGAACACTGACAACACTAATTTCAATTTTCTTTCTCTCTCCTCTTCTCCTTCTCCGGTTTCGCTTTCTCTCTCCTCGTCGATCCCAAATCCATCAAGAGAAGATTTAATGGGTGCCGCGCAAGTGCAAAACGGCGTCGCCGCTTCTTCCTCTCCTTTGAACCAAGGAACAGCGACTGCGGCAACAACAACAGTAACACACGGCGTCGCCGCCTCTCCATCCTCCTCTTTGAACCAAGCAACAGCGACTGCAACAACAACAGCAGCAGCAGCAACAGCGTGTGAAGTGTCGAATGCGCCGAAGAAGAAGAAAATCTGCTGTGCTTGTCCAGACACGAAGAGGCTTCGGGATGAGTGCATCGTGGAGAACGGAGAAGACGCTTGTGCCAAATGGATCGAAGCTCATCGACTCTGTCTCCGATCCGAGGGATTCAATGTTTGAGAATCGGTATTTTTATTGGAATAATAAGAACTACACTTTTTTGTTGTTGTTGAAATTAGATAATAGGTCAAAATAAAATTTCAAGGGATTTAGGGTTTATCAAATTTTGCTTAAAATTCTTAATTTGTATACTGCCTCCTGTATTCAGGAAATTGTGATTTTTAGACCTAACACACCATATTCTATCAAAATGTTGGTCCATTTGATCTGATATAAACC
TARGET=335,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5428
PRIMER_LEFT_PENALTY=0.001941
PRIMER_RIGHT_PENALTY=0.540881
PRIMER_LEFT_SEQUENCE=AACAACAACAGTAACACACGGC
PRIMER_RIGHT_SEQUENCE=GGAGACAGAGTCGATGAGCTTC
PRIMER_LEFT=257,22
PRIMER_RIGHT=492,22
PRIMER_LEFT_TM=60.002
PRIMER_RIGHT_TM=60.541
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.6746
PRIMER_LEFT_1_PENALTY=0.001941
PRIMER_RIGHT_1_PENALTY=0.672617
PRIMER_LEFT_1_SEQUENCE=AACAACAACAGTAACACACGGC
PRIMER_RIGHT_1_SEQUENCE=CGATTCTCAAACATTGAATCCC
PRIMER_LEFT_1=257,22
PRIMER_RIGHT_1=521,22
PRIMER_LEFT_1_TM=60.002
PRIMER_RIGHT_1_TM=60.673
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.6746
PRIMER_LEFT_2_PENALTY=0.001941
PRIMER_RIGHT_2_PENALTY=0.672617
PRIMER_LEFT_2_SEQUENCE=AACAACAACAGTAACACACGGC
PRIMER_RIGHT_2_SEQUENCE=CCGATTCTCAAACATTGAATCC
PRIMER_LEFT_2=257,22
PRIMER_RIGHT_2=522,22
PRIMER_LEFT_2_TM=60.002
PRIMER_RIGHT_2_TM=60.673
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.6831
PRIMER_LEFT_3_PENALTY=0.142170
PRIMER_RIGHT_3_PENALTY=0.540881
PRIMER_LEFT_3_SEQUENCE=CTTCTTCCTCTCCTTTGAACCA
PRIMER_RIGHT_3_SEQUENCE=GGAGACAGAGTCGATGAGCTTC
PRIMER_LEFT_3=217,22
PRIMER_RIGHT_3=492,22
PRIMER_LEFT_3_TM=59.858
PRIMER_RIGHT_3_TM=60.541
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.7545
PRIMER_LEFT_4_PENALTY=0.213575
PRIMER_RIGHT_4_PENALTY=0.540881
PRIMER_LEFT_4_SEQUENCE=TTCTTCCTCTCCTTTGAACCAA
PRIMER_RIGHT_4_SEQUENCE=GGAGACAGAGTCGATGAGCTTC
PRIMER_LEFT_4=218,22
PRIMER_RIGHT_4=492,22
PRIMER_LEFT_4_TM=60.214
PRIMER_RIGHT_4_TM=60.541
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_04437 Ps_contig_mira-and-tgicl-ass_franssen_33215
SEQUENCE=AAAACAACGTTCGTTGGCAAAACAAAGAGAAAGAAACTTTTTCAAAGGGGAGGAGAAGAGAAAGAGAAAGAGAAACAGAACACTGACAACACTAATTTCAATTTTCTTTCTCTCTCCTCTTCTCCTTCTCCGGTTTCGCTTTCTCTCTCCTCGTCGATCCCAAATCCATCAAGAGAAGATTTAATGGGTGCCGCGCAAGTGCAAAACGGCGTCGCCGCTTCTTCCTCTCCTTTGAACCAAGGAACAGCGACTGCGGCAACAACAACAGTAACACACGGCGTCGCCGCCTCTCCATCCTCCTCTTTGAACCAAGCAACAGCGACTGCAACAACAACAGCAGCAGCAGCAACAGCGTGTGAAGTGTCGAATGCGCCGAAGAAGAAGAAAATCTGCTGTGCTTGTCCAGACACGAAGAGGCTTCGGGATGAGTGCATCGTGGAGAACGGAGAAGACGCTTGTGCCAAATGGATCGAAGCTCATCGACTCTGTCTCCGATCCGAGGGATTCAATGTTTGAGAATCGGTATTTTTATTGGAATAATAAGAACTACACTTTTTTGTTGTTGTTGAAATTAGATAATAGGTCAAAATAAAATTTCAAGGGATTTAGGGTTTATCAAATTTTGCTTAAAATTCTTAATTTGTATACTGCCTCCTGTATTCAGGAAATTGTGATTTTTAGACCTAACACACCATATTCTATCAAAATGTTGGTCCATTTGATCTGATATAAACC
TARGET=375,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5428
PRIMER_LEFT_PENALTY=0.001941
PRIMER_RIGHT_PENALTY=0.540881
PRIMER_LEFT_SEQUENCE=AACAACAACAGTAACACACGGC
PRIMER_RIGHT_SEQUENCE=GGAGACAGAGTCGATGAGCTTC
PRIMER_LEFT=257,22
PRIMER_RIGHT=492,22
PRIMER_LEFT_TM=60.002
PRIMER_RIGHT_TM=60.541
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.6746
PRIMER_LEFT_1_PENALTY=0.001941
PRIMER_RIGHT_1_PENALTY=0.672617
PRIMER_LEFT_1_SEQUENCE=AACAACAACAGTAACACACGGC
PRIMER_RIGHT_1_SEQUENCE=CGATTCTCAAACATTGAATCCC
PRIMER_LEFT_1=257,22
PRIMER_RIGHT_1=521,22
PRIMER_LEFT_1_TM=60.002
PRIMER_RIGHT_1_TM=60.673
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.6746
PRIMER_LEFT_2_PENALTY=0.001941
PRIMER_RIGHT_2_PENALTY=0.672617
PRIMER_LEFT_2_SEQUENCE=AACAACAACAGTAACACACGGC
PRIMER_RIGHT_2_SEQUENCE=CCGATTCTCAAACATTGAATCC
PRIMER_LEFT_2=257,22
PRIMER_RIGHT_2=522,22
PRIMER_LEFT_2_TM=60.002
PRIMER_RIGHT_2_TM=60.673
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.6831
PRIMER_LEFT_3_PENALTY=0.142170
PRIMER_RIGHT_3_PENALTY=0.540881
PRIMER_LEFT_3_SEQUENCE=CTTCTTCCTCTCCTTTGAACCA
PRIMER_RIGHT_3_SEQUENCE=GGAGACAGAGTCGATGAGCTTC
PRIMER_LEFT_3=217,22
PRIMER_RIGHT_3=492,22
PRIMER_LEFT_3_TM=59.858
PRIMER_RIGHT_3_TM=60.541
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.7545
PRIMER_LEFT_4_PENALTY=0.213575
PRIMER_RIGHT_4_PENALTY=0.540881
PRIMER_LEFT_4_SEQUENCE=TTCTTCCTCTCCTTTGAACCAA
PRIMER_RIGHT_4_SEQUENCE=GGAGACAGAGTCGATGAGCTTC
PRIMER_LEFT_4=218,22
PRIMER_RIGHT_4=492,22
PRIMER_LEFT_4_TM=60.214
PRIMER_RIGHT_4_TM=60.541
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_04438 Ps_contig_mira-and-tgicl-ass_franssen_33215
SEQUENCE=AAAACAACGTTCGTTGGCAAAACAAAGAGAAAGAAACTTTTTCAAAGGGGAGGAGAAGAGAAAGAGAAAGAGAAACAGAACACTGACAACACTAATTTCAATTTTCTTTCTCTCTCCTCTTCTCCTTCTCCGGTTTCGCTTTCTCTCTCCTCGTCGATCCCAAATCCATCAAGAGAAGATTTAATGGGTGCCGCGCAAGTGCAAAACGGCGTCGCCGCTTCTTCCTCTCCTTTGAACCAAGGAACAGCGACTGCGGCAACAACAACAGTAACACACGGCGTCGCCGCCTCTCCATCCTCCTCTTTGAACCAAGCAACAGCGACTGCAACAACAACAGCAGCAGCAGCAACAGCGTGTGAAGTGTCGAATGCGCCGAAGAAGAAGAAAATCTGCTGTGCTTGTCCAGACACGAAGAGGCTTCGGGATGAGTGCATCGTGGAGAACGGAGAAGACGCTTGTGCCAAATGGATCGAAGCTCATCGACTCTGTCTCCGATCCGAGGGATTCAATGTTTGAGAATCGGTATTTTTATTGGAATAATAAGAACTACACTTTTTTGTTGTTGTTGAAATTAGATAATAGGTCAAAATAAAATTTCAAGGGATTTAGGGTTTATCAAATTTTGCTTAAAATTCTTAATTTGTATACTGCCTCCTGTATTCAGGAAATTGTGATTTTTAGACCTAACACACCATATTCTATCAAAATGTTGGTCCATTTGATCTGATATAAACC
TARGET=557,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.7081
PRIMER_LEFT_PENALTY=0.540881
PRIMER_RIGHT_PENALTY=4.167228
PRIMER_LEFT_SEQUENCE=GAAGCTCATCGACTCTGTCTCC
PRIMER_RIGHT_SEQUENCE=CTGAATACAGGAGGCAGTATACAAA
PRIMER_LEFT=471,22
PRIMER_RIGHT=663,25
PRIMER_LEFT_TM=60.541
PRIMER_RIGHT_TM=58.833
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=193
PRIMER_PAIR_PENALTY_1=4.8398
PRIMER_LEFT_1_PENALTY=0.672617
PRIMER_RIGHT_1_PENALTY=4.167228
PRIMER_LEFT_1_SEQUENCE=GGATTCAATGTTTGAGAATCGG
PRIMER_RIGHT_1_SEQUENCE=CTGAATACAGGAGGCAGTATACAAA
PRIMER_LEFT_1=501,22
PRIMER_RIGHT_1=663,25
PRIMER_LEFT_1_TM=60.673
PRIMER_RIGHT_1_TM=58.833
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.8000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=4.8398
PRIMER_LEFT_2_PENALTY=0.672617
PRIMER_RIGHT_2_PENALTY=4.167228
PRIMER_LEFT_2_SEQUENCE=GGGATTCAATGTTTGAGAATCG
PRIMER_RIGHT_2_SEQUENCE=CTGAATACAGGAGGCAGTATACAAA
PRIMER_LEFT_2=500,22
PRIMER_RIGHT_2=663,25
PRIMER_LEFT_2_TM=60.673
PRIMER_RIGHT_2_TM=58.833
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=4.9691
PRIMER_LEFT_3_PENALTY=0.801836
PRIMER_RIGHT_3_PENALTY=4.167228
PRIMER_LEFT_3_SEQUENCE=AGAAAATCTGCTGTGCTTGTCC
PRIMER_RIGHT_3_SEQUENCE=CTGAATACAGGAGGCAGTATACAAA
PRIMER_LEFT_3=382,22
PRIMER_RIGHT_3=663,25
PRIMER_LEFT_3_TM=60.802
PRIMER_RIGHT_3_TM=58.833
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=5.1004
PRIMER_LEFT_4_PENALTY=0.933135
PRIMER_RIGHT_4_PENALTY=4.167228
PRIMER_LEFT_4_SEQUENCE=GATCGAAGCTCATCGACTCTGT
PRIMER_RIGHT_4_SEQUENCE=CTGAATACAGGAGGCAGTATACAAA
PRIMER_LEFT_4=467,22
PRIMER_RIGHT_4=663,25
PRIMER_LEFT_4_TM=60.933
PRIMER_RIGHT_4_TM=58.833
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=197
=
PRIMER_SEQUENCE_ID=SSR_04439 Ps_contig_mira-and-tgicl-ass_franssen_33218
SEQUENCE=TAAGTCTAGTTGGTTATTCAACGCAGAGTACGCGGACAATTAAAAAGAACACAAAAAGTAAAAGAAAATATTCGCAAACCTCGGAGGAGATTCCGAAGCTTTTTCGCTTTTGCCCTCATTTTCTATACCTAAAATTGTTTTCACTGTTTTTGTATTTCTCTGTATCATCTGTCACCGTCACTCCCCTCTATAAATTCACACCATCTTACCTATCAAACTTCCCCTTCTTCCTCGAAGAAAATCTACTGCTTCTGAGGAATCTTGATGCTTCTGATTGAAAGTTTTTGACATTCATGTCTCATGTTCTCAGTGAAGTTCTTCTTTCTGGATTCATCATAAATTCCTCTTTCAGACGCAGAACTCACTTAGTTCAATCTTTCTCCGTCGTCTTCCTCTATTGGTTCTACGTTTTCTCAATTCACAGTTTCAATTCATATAGTAGCATGTAAATCATCTTATTAGTACTAATTATTTAGTTAATTTTTGCGATTAGGGTTTTCGAGTTTTTTGTTGTTGTTATTGTTGTTTTGTAAGTGTAATAGTGTTCCATGGCTTCCTCCGGCGGATCGACGAGGAGTTCTGGATCTGAGAAGGATTTGAACAATTTGATGGATGAGAGAAAGAACAAGAGGAAGCAATCGAATCGGGAATCGGCGAAGAGATCTCGGATGAGGAAGCAGAAGCATGTTGATGATATGATGAATCAGGTATCTGAGCTCACAAAAGACAATAATGAAATTGTGAATAAGATAAATATCACCACAGAGCAGTATTTGAATGTTGAGGCTGAAAATTCAATTTTGAGGGCCCAAATAGGTGAGCTTAGTCAAAGGTTTCAATCATTGAATAACATTATTGAACTCATCAACACAAACAACTCTAGTAATGGGTCCTATGATTCAAGGGATTGTTATGAAACAAGTGCTCAAAACAGGATGAATTTGATGATGTATAATAATAACCAGCCCATTACAACTTCTGCACATGTTTTTAAGTGGTGATCAGTGATGAATGATGATGATGATGGGAAAATGTAATACTAGTAAAATAAAAAGGGTGGAATTGGAATGATTTGGGTTGAGTGAAAGGGTGATAAGTAAGAAATAATGATGGTAGTAGTGTGTGATTAAGGGAAGGCATTGCTTGGTGAGAAATGGTGAAGTACACTACTCAATGAAAATGACTTAGTGGTTGGTACTATGTCACTGATCACTAGTTCTACTTTGTCCATTACCATGAGGGGATATTTTGGTGACTGTAATCTATATTTCTCTATGTTGTTTTTTTTATTATGTTATTTTGCTCAGTGTAAAAAATTATCTTTTTTTAATGGAAAATAATTATTTTTTTTGGTTNACT
TARGET=1012,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4409
PRIMER_LEFT_PENALTY=0.219884
PRIMER_RIGHT_PENALTY=0.220992
PRIMER_LEFT_SEQUENCE=ACAAGTGCTCAAAACAGGATGA
PRIMER_RIGHT_SEQUENCE=TTATCACCCTTTCACTCAACCC
PRIMER_LEFT=917,22
PRIMER_RIGHT=1095,22
PRIMER_LEFT_TM=59.780
PRIMER_RIGHT_TM=60.221
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.4690
PRIMER_LEFT_1_PENALTY=0.219884
PRIMER_RIGHT_1_PENALTY=0.249074
PRIMER_LEFT_1_SEQUENCE=ACAAGTGCTCAAAACAGGATGA
PRIMER_RIGHT_1_SEQUENCE=ATGCCTTCCCTTAATCACACAC
PRIMER_LEFT_1=917,22
PRIMER_RIGHT_1=1139,22
PRIMER_LEFT_1_TM=59.780
PRIMER_RIGHT_1_TM=60.249
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.4953
PRIMER_LEFT_2_PENALTY=0.404566
PRIMER_RIGHT_2_PENALTY=0.090770
PRIMER_LEFT_2_SEQUENCE=AACCAGCCCATTACAACTTCTG
PRIMER_RIGHT_2_SEQUENCE=CCAAAATATCCCCTCATGGTAA
PRIMER_LEFT_2=959,22
PRIMER_RIGHT_2=1251,22
PRIMER_LEFT_2_TM=60.405
PRIMER_RIGHT_2_TM=59.909
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.5047
PRIMER_LEFT_3_PENALTY=0.283676
PRIMER_RIGHT_3_PENALTY=0.220992
PRIMER_LEFT_3_SEQUENCE=CAAGTGCTCAAAACAGGATGAA
PRIMER_RIGHT_3_SEQUENCE=TTATCACCCTTTCACTCAACCC
PRIMER_LEFT_3=918,22
PRIMER_RIGHT_3=1095,22
PRIMER_LEFT_3_TM=60.284
PRIMER_RIGHT_3_TM=60.221
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=178
PRIMER_PAIR_PENALTY_4=0.5047
PRIMER_LEFT_4_PENALTY=0.283676
PRIMER_RIGHT_4_PENALTY=0.220992
PRIMER_LEFT_4_SEQUENCE=CAAGTGCTCAAAACAGGATGAA
PRIMER_RIGHT_4_SEQUENCE=CCTTTCACTCAACCCAAATCAT
PRIMER_LEFT_4=918,22
PRIMER_RIGHT_4=1088,22
PRIMER_LEFT_4_TM=60.284
PRIMER_RIGHT_4_TM=60.221
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_04440 Ps_contig_mira-and-tgicl-ass_franssen_33226
SEQUENCE=CAACCGAGGAGTGTGTCTGTAAGGAACAGTGTGAGTGAAGAAAGAGATAGAGAGAGAGATATAAACACAGCGCGCACCATTCTCCACTGTCCAAGGAGATCACGCACCGAACCTAACACTATTCTCTCTCTTTCTCTCTCTCTAGTGTTCGGTGCTTCAAGTTCAAGACTACTCTAGCTAGTTCTATACTCTTGTTTTCTTATTCTTCATTGTTTACTTTTTATTAATGTTGAGTTGATTTGTAAGATTCGGGTTGGGACTTTGTGTGACCCGAGTAATGGATCCGGGTTCGATGATGAACGAGGGTTCATTTCCGAATGGGAGTGGGAATACGACGCCGTTTAGTTTAGCGGAGATCTGGCAGTTTCCAGCTGCAATTAATGGCGGCGGAGGTGGATTGGGACTTAGAAGGCCGCAATTTGGAACCGGTTTGGGACAGTTTGGTGAATTTGGAACCGGTTCGAACCGGGACGTGGATGGACCGGACCCGAGAGTTGTTTTGAACCATGGTGTTGTTGGTGGTAAGAAGAGGCGTGACTCGGAAGATGTTGATTCACCTAAGTGTGTTTCCACCAGTAATGGCGGTGCCAATGCCGTGAATGATATTGATGGAAAACGGTCTAAAGCATTAGGGAACAGAATTGATGATGGTAAAGTTGAAGGAGAAGCCAGTTCAGGAAAGCCTGCGGAGCAAAGCAGTAAGCCGCCTCCTCCCGACCCTCCGAAGCAAGATTACATCCACGTCCGAGCTAGAAGGGGTCAAGCTACTGATAGCCATAGTCTTGCAGAACGCGCTAGAAGGGAAAAGATTAGTGAAAGGATGAAAATTCTTCAGGATTTAGTTCCTGGTTGTAATAAGGTTATTGGGAAAGCATTGGTCCTTGATGAAATAATTAATTATATTCAATCACTTCAGCGCCAAGTGGAGTTTTTGTCAATGAAACTTGAAGCAGTTAATTCGAGACTCAACACTGGCATTGAAGTCTTTCCTCCAAAAGATTATGGTCAACAAACATATGATCTAGCCGGCATGCCATTTGTTTCACAAGCTACAAGAGAGCCAAGCAGAGGATCATCCCCAGAATGGTTACATATGCAGGTAGGTGGCGGTTTCGAAAGAACATCGTAGGGTACGAGAACCCTTCACTGTGTAATAATAAAATGCATAAACGAGCAGTATTCCATTCCATTTCATCCCATCTCATTCCATCCATCGATTGCTCCTATATTATAAAACAAAAATGATATACTTAAGGTGTAAAAGTCCAATGGACACTTCATTCCCATTTTCTACACAAAAGAATGGAGTTGTTTCTGTTTTCTACAACAGGATTGCTTCAGTTTGTGGTTTAGCTTTTGAAAATGAATTCATTTACTCAATATTGTGTACTATATAGTGGACTTTCAGGTTTTTCTTTCTTGGTATTTAACGTGACTTATAAGTGATTATTCTTGTATAAGAATGCTGCTTGTTTTGAATACATAATGCTTACATTTTGTGTAAGNAGTNAAA
TARGET=49,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2532
PRIMER_LEFT_PENALTY=0.207335
PRIMER_RIGHT_PENALTY=0.045851
PRIMER_LEFT_SEQUENCE=CAACCGAGGAGTGTGTCTGTAA
PRIMER_RIGHT_SEQUENCE=AATAGTGTTAGGTTCGGTGCGT
PRIMER_LEFT=0,22
PRIMER_RIGHT=122,22
PRIMER_LEFT_TM=60.207
PRIMER_RIGHT_TM=59.954
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=0.2595
PRIMER_LEFT_1_PENALTY=0.207335
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=CAACCGAGGAGTGTGTCTGTAA
PRIMER_RIGHT_1_SEQUENCE=TTGAAGCACCGAACACTAGAGA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=159,22
PRIMER_LEFT_1_TM=60.207
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.5280
PRIMER_LEFT_2_PENALTY=0.207335
PRIMER_RIGHT_2_PENALTY=0.320679
PRIMER_LEFT_2_SEQUENCE=CAACCGAGGAGTGTGTCTGTAA
PRIMER_RIGHT_2_SEQUENCE=TGAAGCACCGAACACTAGAGAG
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=158,22
PRIMER_LEFT_2_TM=60.207
PRIMER_RIGHT_2_TM=59.679
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.6425
PRIMER_LEFT_3_PENALTY=0.596612
PRIMER_RIGHT_3_PENALTY=0.045851
PRIMER_LEFT_3_SEQUENCE=ACCGAGGAGTGTGTCTGTAAGG
PRIMER_RIGHT_3_SEQUENCE=AATAGTGTTAGGTTCGGTGCGT
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=122,22
PRIMER_LEFT_3_TM=60.597
PRIMER_RIGHT_3_TM=59.954
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=121
PRIMER_PAIR_PENALTY_4=0.6488
PRIMER_LEFT_4_PENALTY=0.596612
PRIMER_RIGHT_4_PENALTY=0.052187
PRIMER_LEFT_4_SEQUENCE=ACCGAGGAGTGTGTCTGTAAGG
PRIMER_RIGHT_4_SEQUENCE=TTGAAGCACCGAACACTAGAGA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=159,22
PRIMER_LEFT_4_TM=60.597
PRIMER_RIGHT_4_TM=60.052
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_04441 Ps_contig_mira-and-tgicl-ass_franssen_33226
SEQUENCE=CAACCGAGGAGTGTGTCTGTAAGGAACAGTGTGAGTGAAGAAAGAGATAGAGAGAGAGATATAAACACAGCGCGCACCATTCTCCACTGTCCAAGGAGATCACGCACCGAACCTAACACTATTCTCTCTCTTTCTCTCTCTCTAGTGTTCGGTGCTTCAAGTTCAAGACTACTCTAGCTAGTTCTATACTCTTGTTTTCTTATTCTTCATTGTTTACTTTTTATTAATGTTGAGTTGATTTGTAAGATTCGGGTTGGGACTTTGTGTGACCCGAGTAATGGATCCGGGTTCGATGATGAACGAGGGTTCATTTCCGAATGGGAGTGGGAATACGACGCCGTTTAGTTTAGCGGAGATCTGGCAGTTTCCAGCTGCAATTAATGGCGGCGGAGGTGGATTGGGACTTAGAAGGCCGCAATTTGGAACCGGTTTGGGACAGTTTGGTGAATTTGGAACCGGTTCGAACCGGGACGTGGATGGACCGGACCCGAGAGTTGTTTTGAACCATGGTGTTGTTGGTGGTAAGAAGAGGCGTGACTCGGAAGATGTTGATTCACCTAAGTGTGTTTCCACCAGTAATGGCGGTGCCAATGCCGTGAATGATATTGATGGAAAACGGTCTAAAGCATTAGGGAACAGAATTGATGATGGTAAAGTTGAAGGAGAAGCCAGTTCAGGAAAGCCTGCGGAGCAAAGCAGTAAGCCGCCTCCTCCCGACCCTCCGAAGCAAGATTACATCCACGTCCGAGCTAGAAGGGGTCAAGCTACTGATAGCCATAGTCTTGCAGAACGCGCTAGAAGGGAAAAGATTAGTGAAAGGATGAAAATTCTTCAGGATTTAGTTCCTGGTTGTAATAAGGTTATTGGGAAAGCATTGGTCCTTGATGAAATAATTAATTATATTCAATCACTTCAGCGCCAAGTGGAGTTTTTGTCAATGAAACTTGAAGCAGTTAATTCGAGACTCAACACTGGCATTGAAGTCTTTCCTCCAAAAGATTATGGTCAACAAACATATGATCTAGCCGGCATGCCATTTGTTTCACAAGCTACAAGAGAGCCAAGCAGAGGATCATCCCCAGAATGGTTACATATGCAGGTAGGTGGCGGTTTCGAAAGAACATCGTAGGGTACGAGAACCCTTCACTGTGTAATAATAAAATGCATAAACGAGCAGTATTCCATTCCATTTCATCCCATCTCATTCCATCCATCGATTGCTCCTATATTATAAAACAAAAATGATATACTTAAGGTGTAAAAGTCCAATGGACACTTCATTCCCATTTTCTACACAAAAGAATGGAGTTGTTTCTGTTTTCTACAACAGGATTGCTTCAGTTTGTGGTTTAGCTTTTGAAAATGAATTCATTTACTCAATATTGTGTACTATATAGTGGACTTTCAGGTTTTTCTTTCTTGGTATTTAACGTGACTTATAAGTGATTATTCTTGTATAAGAATGCTGCTTGTTTTGAATACATAATGCTTACATTTTGTGTAAGNAGTNAAA
TARGET=133,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1616
PRIMER_LEFT_PENALTY=0.045851
PRIMER_RIGHT_PENALTY=0.115779
PRIMER_LEFT_SEQUENCE=ACGCACCGAACCTAACACTATT
PRIMER_RIGHT_SEQUENCE=GCCAGATCTCCGCTAAACTAAA
PRIMER_LEFT=101,22
PRIMER_RIGHT=361,22
PRIMER_LEFT_TM=59.954
PRIMER_RIGHT_TM=59.884
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.2402
PRIMER_LEFT_1_PENALTY=0.045851
PRIMER_RIGHT_1_PENALTY=0.194358
PRIMER_LEFT_1_SEQUENCE=ACGCACCGAACCTAACACTATT
PRIMER_RIGHT_1_SEQUENCE=AAATGAACCCTCGTTCATCATC
PRIMER_LEFT_1=101,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=59.954
PRIMER_RIGHT_1_TM=60.194
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.2665
PRIMER_LEFT_2_PENALTY=0.045851
PRIMER_RIGHT_2_PENALTY=0.220659
PRIMER_LEFT_2_SEQUENCE=ACGCACCGAACCTAACACTATT
PRIMER_RIGHT_2_SEQUENCE=CATTAATTGCAGCTGGAAACTG
PRIMER_LEFT_2=101,22
PRIMER_RIGHT_2=382,22
PRIMER_LEFT_2_TM=59.954
PRIMER_RIGHT_2_TM=59.779
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.6163
PRIMER_LEFT_3_PENALTY=0.045851
PRIMER_RIGHT_3_PENALTY=0.570438
PRIMER_LEFT_3_SEQUENCE=ACGCACCGAACCTAACACTATT
PRIMER_RIGHT_3_SEQUENCE=AAAGTCCCAACCCGAATCTTAC
PRIMER_LEFT_3=101,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=59.954
PRIMER_RIGHT_3_TM=60.570
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=0.6502
PRIMER_LEFT_4_PENALTY=0.207335
PRIMER_RIGHT_4_PENALTY=0.442900
PRIMER_LEFT_4_SEQUENCE=CAACCGAGGAGTGTGTCTGTAA
PRIMER_RIGHT_4_SEQUENCE=TAGTCTTGAACTTGAAGCACCG
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=170,22
PRIMER_LEFT_4_TM=60.207
PRIMER_RIGHT_4_TM=59.557
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_04442 Ps_contig_mira-and-tgicl-ass_franssen_33227
SEQUENCE=TGCATATAAACAATTTGTCTAAATTTATGGATTCACACATACAAATACATACATATTCATCATAATGCTCCTAATACAATAATCATCCACCTCCACTCCTTTTCTTTATCAACTCCCTAAACTCATCATCAGTACTTTTAAGCATCAATGACAAAGGCAAGTTAACCCCACTATCAACAACCTCCATGTGTCTAACTTTAATCCTATTCTCCAAACTAAAAGAGAAATACTGAGGAAACTCTTTCAACTCCTCCAACTTCCCTCCCATTTCCACAGAAAAATACTCAAACTTTGGCTGAAAATTATTTTCAATACTAAAAGTCAACAATGCAGGACACCTCAAAACCATACACCTAGTCTCCTCTTTAGTAAATCCCATACTCTCCAAATGCTTAAGCTTAGGAATAAGTGTCCTTTCAACATTGGAAACCAAAAGAACACAATCTTGATAAGCCAGAGCCTCTAAATCCCTTAACCCTAATCTCCTTAAATAAAACAAAGCTGGTTTAAGCTGATCCACCACACTAGAAGTAAGCAATCTTGGACACTTTTTCACAACTTTTCTAAAGCTATGTTCAGGCACTTTAAGTTCATGCACAAGAAAATCAAAAACTGGGTTGAGATCAGTTTTGATGCTTGAAGTGAGAATCTTTGGACACATGCCAAAGATTCTTGGAAGGTCTTTGTGTAGGATTCCTTTGGAGAGAAGGAAGGAGATAATGGAATGAATGGATTCTAATGTAGCTGTATGAAGGTTAGGGTTTTGGGAAAGTGCTTTACCTGAATCAATACCCATTACTTCAAGACACAAGATTTTTTCTTTGAATTGAATTGAAAGCTTTTCATGAGTTGGTGTATAAAGAGGGTGGTTTTGGATGATGAGGCTTTTGGGTTTGGGAGTTAAATGGGTTGTGTTTGGTTTGGGAATTAAATGGGTTGTGTTTGGTTTGGAAATTGATGAAGGAGATTGTGATGATGGTTTATCTAAAGAGACTGTGCATAGTGAAGATGAGTGTAGAGCTATGGTTCTTACAGCTTGGAACATGGTGGTGCTGAGGATGTTTGGATAGTGCTGAGGAT
TARGET=822,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1866
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.185822
PRIMER_LEFT_SEQUENCE=TGGGAAAGTGCTTTACCTGAAT
PRIMER_RIGHT_SEQUENCE=CTCCTTCATCAATTTCCAAACC
PRIMER_LEFT=764,22
PRIMER_RIGHT=965,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.814
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.4949
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.494198
PRIMER_LEFT_1_SEQUENCE=TGGGAAAGTGCTTTACCTGAAT
PRIMER_RIGHT_1_SEQUENCE=ACACAACCCATTTAACTCCCAA
PRIMER_LEFT_1=764,22
PRIMER_RIGHT_1=914,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.494
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=0.4949
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.494198
PRIMER_LEFT_2_SEQUENCE=TGGGAAAGTGCTTTACCTGAAT
PRIMER_RIGHT_2_SEQUENCE=AACACAACCCATTTAACTCCCA
PRIMER_LEFT_2=764,22
PRIMER_RIGHT_2=915,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.494
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.5628
PRIMER_LEFT_3_PENALTY=0.068602
PRIMER_RIGHT_3_PENALTY=0.494198
PRIMER_LEFT_3_SEQUENCE=ATTCCTTTGGAGAGAAGGAAGG
PRIMER_RIGHT_3_SEQUENCE=AACACAACCCATTTAACTCCCA
PRIMER_LEFT_3=692,22
PRIMER_RIGHT_3=915,22
PRIMER_LEFT_3_TM=60.069
PRIMER_RIGHT_3_TM=60.494
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.5628
PRIMER_LEFT_4_PENALTY=0.068602
PRIMER_RIGHT_4_PENALTY=0.494198
PRIMER_LEFT_4_SEQUENCE=ATTCCTTTGGAGAGAAGGAAGG
PRIMER_RIGHT_4_SEQUENCE=ACACAACCCATTTAACTCCCAA
PRIMER_LEFT_4=692,22
PRIMER_RIGHT_4=914,22
PRIMER_LEFT_4_TM=60.069
PRIMER_RIGHT_4_TM=60.494
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_04443 Ps_contig_mira-and-tgicl-ass_franssen_33231
SEQUENCE=CAAAACCCAACACTCTGCAAAAATGGCGAAATCACGGGAGCGAACCAACGCCACCCGTCCCTCCGCCGAACTCATGTCTTCCCCCCGGCCTCGCCATCAAGGTCCCGCCTGCTTCGACGCTCTCAGCGCCAAACTCATCGAATCAGCCGGTTTCTCATACTGCATCACAAGCGGTTTCTCAATTTCCGCCACCAGATTAGGTCTTCCAGACGCCGGTTTCATCTCCTACGGCGAAATCGTCGATCAAGGTCGTCTCATTACTCAATCTGTTAATATTCCTGTTATTGGTGATGGTGATAATGGTTATGGTAATGCCATGAATGTTAAGAGAACTGTTAAGGGTTTTATCGCTGCTGGTTTTGCTGGAATCATTCTCGAAGATCAGGTGGCGCCGAAAGCATGTGGGCACACGCGTGGGAGGAAAGTGGTTTCTAGAGAGGAGGCGGTTATGAAGATTAAAGCTGCAGTTGATGCTAGAACTGAGAGCGGGTCTGATATTGTGATAGTGGCCCGCAGCGATGCCCGACAAGGTGTGTCTCTTGAAGAAGCGCTTTGGAGGTCAAACGCATATGCTGAAGCTGGAGCTGATGTTGTCTTTATTGACGCACTAGGTTCTGTTGAAGAGATGAAAGCTTTTTGTCAAGTTGCTCCTGATGTTCCTCATCTGGCGAATATGCTTGAAGGAGGAGGAAAAACACCAATACTTAATGCTCAGGAACTCGATCAAGTTGGTTATAAACTCGCTGTTTATCCACTTTCTTTGATGGGGGTCTCGATACGAGCAATGCAGGGTGCACTCACTGCCATTAAGGTAGGTGGTATTCCTCCACCGGAAAGCTTGCCATCTTTTGAAGAAATTAAGGAAATTGTTGGTTTCAACGATTATTACAAAGAGGAAGAGCGTTATGCAACAACTACGGATCACCAGCTTTAAGCATTTAGAGAGAAAATCAACAAAATTGAGTCAATAAAAACCCTTTTACTGATGGGGCGGTTTAGTCGTTTCTTCTAAGAGTAGTGTTGTATTGTATTGTATTGTATGTAATGTAATCTAGACCTGCTTTTATCTGCTCAACTTTGTGTATCTGGTTCAGCTTATTAAGATAAATATCTGATCAGTGTTTATCTAAATAAGTTAAATCCATCTAATAAATCATGCATAGTTTTCTTCGGARACARTTAGACTATTATTTTGAATTTTTATGTTTCATCACTAGGGGAATCAATGGCTAATCCACTGTTGCTAAGTA
TARGET=1021,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04444 Ps_contig_mira-and-tgicl-ass_franssen_33240
SEQUENCE=TTAAAAATTCACCAAGCCAAAAATCACATTGACGAGATTGCAATAATAATAATAATATTACATAGATCCATCATCACTTACATAAATGAGACATTGATGCTAAACTACAATACAACCTCTAAACTCACATTCTTTAATTCACTAGAAAAACAAACATATCAGAATACCTCAATCTATATCATCATTATTATTACATTACGTTACCCTTTCTCCTCCGCGTTCTACAAACGCGCTTAATTATTCTTCATAAATTAAAGAAAAAAACACCATATCTCTCGCACATCAACAACATGCAATTCATAAGGTATATGAAATATCCGCCGAATGAATTATGTAGGTGAGTACCAGCGCCAACAACATCAACAAATAAGCTATTCCTTGATCAACCGATGAACCGTCGCTGGTAGGAGCTGGTGCAGGTGCAAGAGATTGAGCGTGAACAGCGGCGGGAACGGAAAGAGCGAAAATGAGAGTGGCGAGGATAGCCACAATTCCGAATCCGAAAGAATGTTTAGTGGAAGCCATATCGGAAAATGCGAATAACGTAATGTGTTATGTTTGTGTAAATTTTGTGAGTGTCTCTGCGGCGTCTCTGTTGAAAACGGAAGAGCCGCTGCAGAGAGAGAAATGAAATGATGATGGAGAGAGAGAGCCCNGAACGACGGAGAGTGATGAGCGGTTTAAAAGGCGGTTATTGGAGGTGTGTGGAGGGAGAGAGAAAGNGGAG
TARGET=42,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7440
PRIMER_LEFT_PENALTY=1.381246
PRIMER_RIGHT_PENALTY=0.362762
PRIMER_LEFT_SEQUENCE=AGCCAAAAATCACATTGACGAG
PRIMER_RIGHT_SEQUENCE=TGTGCGAGAGATATGGTGTTTT
PRIMER_LEFT=14,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=61.381
PRIMER_RIGHT_TM=59.637
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=1.7546
PRIMER_LEFT_1_PENALTY=1.381246
PRIMER_RIGHT_1_PENALTY=0.373358
PRIMER_LEFT_1_SEQUENCE=AGCCAAAAATCACATTGACGAG
PRIMER_RIGHT_1_SEQUENCE=ATGTTGTTGATGTGCGAGAGAT
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=291,22
PRIMER_LEFT_1_TM=61.381
PRIMER_RIGHT_1_TM=59.627
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=1.8587
PRIMER_LEFT_2_PENALTY=1.495889
PRIMER_RIGHT_2_PENALTY=0.362762
PRIMER_LEFT_2_SEQUENCE=GCCAAAAATCACATTGACGAG
PRIMER_RIGHT_2_SEQUENCE=TGTGCGAGAGATATGGTGTTTT
PRIMER_LEFT_2=15,21
PRIMER_RIGHT_2=281,22
PRIMER_LEFT_2_TM=60.496
PRIMER_RIGHT_2_TM=59.637
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=1.8692
PRIMER_LEFT_3_PENALTY=1.495889
PRIMER_RIGHT_3_PENALTY=0.373358
PRIMER_LEFT_3_SEQUENCE=GCCAAAAATCACATTGACGAG
PRIMER_RIGHT_3_SEQUENCE=ATGTTGTTGATGTGCGAGAGAT
PRIMER_LEFT_3=15,21
PRIMER_RIGHT_3=291,22
PRIMER_LEFT_3_TM=60.496
PRIMER_RIGHT_3_TM=59.627
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=1.9286
PRIMER_LEFT_4_PENALTY=1.381246
PRIMER_RIGHT_4_PENALTY=0.547379
PRIMER_LEFT_4_SEQUENCE=AGCCAAAAATCACATTGACGAG
PRIMER_RIGHT_4_SEQUENCE=TGATGTGCGAGAGATATGGTGT
PRIMER_LEFT_4=14,22
PRIMER_RIGHT_4=284,22
PRIMER_LEFT_4_TM=61.381
PRIMER_RIGHT_4_TM=60.547
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_04445 Ps_contig_mira-and-tgicl-ass_franssen_33240
SEQUENCE=TTAAAAATTCACCAAGCCAAAAATCACATTGACGAGATTGCAATAATAATAATAATATTACATAGATCCATCATCACTTACATAAATGAGACATTGATGCTAAACTACAATACAACCTCTAAACTCACATTCTTTAATTCACTAGAAAAACAAACATATCAGAATACCTCAATCTATATCATCATTATTATTACATTACGTTACCCTTTCTCCTCCGCGTTCTACAAACGCGCTTAATTATTCTTCATAAATTAAAGAAAAAAACACCATATCTCTCGCACATCAACAACATGCAATTCATAAGGTATATGAAATATCCGCCGAATGAATTATGTAGGTGAGTACCAGCGCCAACAACATCAACAAATAAGCTATTCCTTGATCAACCGATGAACCGTCGCTGGTAGGAGCTGGTGCAGGTGCAAGAGATTGAGCGTGAACAGCGGCGGGAACGGAAAGAGCGAAAATGAGAGTGGCGAGGATAGCCACAATTCCGAATCCGAAAGAATGTTTAGTGGAAGCCATATCGGAAAATGCGAATAACGTAATGTGTTATGTTTGTGTAAATTTTGTGAGTGTCTCTGCGGCGTCTCTGTTGAAAACGGAAGAGCCGCTGCAGAGAGAGAAATGAAATGATGATGGAGAGAGAGAGCCCNGAACGACGGAGAGTGATGAGCGGTTTAAAAGGCGGTTATTGGAGGTGTGTGGAGGGAGAGAGAAAGNGGAG
TARGET=642,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4610
PRIMER_LEFT_PENALTY=0.331942
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=GTTTAGTGGAAGCCATATCGGA
PRIMER_RIGHT_SEQUENCE=CCTTTTAAACCGCTCATCACTC
PRIMER_LEFT=509,22
PRIMER_RIGHT=687,22
PRIMER_LEFT_TM=60.332
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.6197
PRIMER_LEFT_1_PENALTY=0.331942
PRIMER_RIGHT_1_PENALTY=0.287765
PRIMER_LEFT_1_SEQUENCE=GTTTAGTGGAAGCCATATCGGA
PRIMER_RIGHT_1_SEQUENCE=CTTTCTCTCTCCCTCCACACAC
PRIMER_LEFT_1=509,22
PRIMER_RIGHT_1=721,22
PRIMER_LEFT_1_TM=60.332
PRIMER_RIGHT_1_TM=60.288
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.6603
PRIMER_LEFT_2_PENALTY=0.372557
PRIMER_RIGHT_2_PENALTY=0.287765
PRIMER_LEFT_2_SEQUENCE=GGAAAGAGCGAAAATGAGAGTG
PRIMER_RIGHT_2_SEQUENCE=CTTTCTCTCTCCCTCCACACAC
PRIMER_LEFT_2=453,22
PRIMER_RIGHT_2=721,22
PRIMER_LEFT_2_TM=60.373
PRIMER_RIGHT_2_TM=60.288
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=0.8633
PRIMER_LEFT_3_PENALTY=0.575571
PRIMER_RIGHT_3_PENALTY=0.287765
PRIMER_LEFT_3_SEQUENCE=AAATTTTGTGAGTGTCTCTGCG
PRIMER_RIGHT_3_SEQUENCE=CTTTCTCTCTCCCTCCACACAC
PRIMER_LEFT_3=564,22
PRIMER_RIGHT_3=721,22
PRIMER_LEFT_3_TM=59.424
PRIMER_RIGHT_3_TM=60.288
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=0.9175
PRIMER_LEFT_4_PENALTY=0.788437
PRIMER_RIGHT_4_PENALTY=0.129103
PRIMER_LEFT_4_SEQUENCE=TAGTGGAAGCCATATCGGAAAA
PRIMER_RIGHT_4_SEQUENCE=CCTTTTAAACCGCTCATCACTC
PRIMER_LEFT_4=512,22
PRIMER_RIGHT_4=687,22
PRIMER_LEFT_4_TM=60.788
PRIMER_RIGHT_4_TM=60.129
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=176
=
PRIMER_SEQUENCE_ID=SSR_04446 Ps_contig_mira-and-tgicl-ass_franssen_33244
SEQUENCE=GACACTCTTTTCGTTTCTCAACAACGTTTTCGTAAACACCTCTGAATCCTGAAAAATATCTCAACTCAACCATTTTGTTTCTTTACGACAAACAAATCTCGACCGTTGGTAATCGCGTCTTGCTGGAATCTTACAAACCCACCGCTTCCCTGTTCCTCTCCCTCAATAAAATCTCTGTTTTCTCTCTCTTTATTCTCGACAACAAAAATGGCGTCTCTCTCTCACCTCAACCAGCTTCTCCACACCAACACCCTTCCAGTCACACCTCTCAACTCCACACCTTCAATCTCTCCCCCGACCACCACCGTCGGACTCCGAAGCAAACAGTTCGGAAGACCGATTGTGGTGGCAGATGTTTCGAGGCGGATGGTTTACGTTCGGTCTAACACGAACGACGATGAGTGGGGTACAGAGAAGGAGGTGCCTTCTCCGGCTGTGGCTGTTGCAGAGGTGAAGTTGACGGATAGTGAGACGGAGAAGCTGAAGAAGGCTTTGGTTGGGTCGTTTTATGGGACGGATCGTGGGTTGAAGGCGACGAGTGAAACGAGAGCGGAGATTGTTGAGCTTATTACTCAGCTTGAGGCTAAGAATCCAACTCCTGCTTCTAATGATGCTTTGACTCTGCTTAATGGAAAATGGATTCTCGCGTATACATCCTTTGCAGGTTTATTTCCATTGTTGTCGAGTGGACTACTGCCATTACTTAAGGTTGAAGAAATATCTCAGACTATTGACTCTGAAAGTTTAACTGTCCAAAACTCTGTTCAGTTTGCCGGTCCATTGACAACCACATCGATCAGTACCAATGCCAAATTTGAGGTTCGAAGTCCGAATCGTTTGCAGATCAAGTTTGAGGAAGGTGTCATAGGAACTCCACAACTGACAGACTCATTAGAGATACCAGAAAATGTGGAATTCTTGGGTCAGAAAATTGATCTATCACCTTTCAAAGGCATACTTACTTCTGTCCAAAGCACTGCTTCATCAGTTGTGCAAACAATTTCCAATCAGCCTCCATTGAAGATTCCAATCTCCAACAGCAAAGCTCAATCTTGGTTGCTTACTACATATCTCGATGAAGAGCTGCGTATCTCAAGAGGTGATGGTGGCAGTGTGTTTGTTCTTATCAAAGAAGGAAGTTCTCTTTTGACAAACTAAAGAAAGTATAGTGGTTCACTTTCTTGGTCATTGTATATGTAATAATTTCCAGTTGTTCGGTTGTTCTTGTATATTCATGATAAGCAACACTGGATTAGATACAAATATGTTATAGTACTTGATAGTTTGGGAATCAAGATCTGAACTAAATTAATAGAAAAAATTGTGGAAATGACTTGTTATCTAATGTCTGGGACTCACAGTTTTGTATTTGTATCATCTGTTACATATTTTAAGTATATTAGTTTTATTTTGTTAGAA
TARGET=214,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0063
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=CTTGCTGGAATCTTACAAACCC
PRIMER_RIGHT_SEQUENCE=CCTTCTCTGTACCCCACTCATC
PRIMER_LEFT=118,22
PRIMER_RIGHT=417,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.0744
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.073614
PRIMER_LEFT_1_SEQUENCE=CTTGCTGGAATCTTACAAACCC
PRIMER_RIGHT_1_SEQUENCE=AAGGTGTGGAGTTGAGAGGTGT
PRIMER_LEFT_1=118,22
PRIMER_RIGHT_1=282,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=0.1979
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.197140
PRIMER_LEFT_2_SEQUENCE=CTTGCTGGAATCTTACAAACCC
PRIMER_RIGHT_2_SEQUENCE=ACTCATCGTCGTTCGTGTTAGA
PRIMER_LEFT_2=118,22
PRIMER_RIGHT_2=402,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=59.803
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.1979
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.197140
PRIMER_LEFT_3_SEQUENCE=CTTGCTGGAATCTTACAAACCC
PRIMER_RIGHT_3_SEQUENCE=CTCATCGTCGTTCGTGTTAGAC
PRIMER_LEFT_3=118,22
PRIMER_RIGHT_3=401,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.2020
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.201241
PRIMER_LEFT_4_SEQUENCE=CTTGCTGGAATCTTACAAACCC
PRIMER_RIGHT_4_SEQUENCE=TGTGGAGTTGAGAGGTGTGACT
PRIMER_LEFT_4=118,22
PRIMER_RIGHT_4=278,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.799
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_04447 Ps_contig_mira-and-tgicl-ass_franssen_33246
SEQUENCE=ATTAATAAAAACTCTATATAAGCAACTTTGTTCATTTGAATTCCAAGCACAATGTTGATATTTAGTGCATATAAGCAGTTGATTAAAATCAGAAAATAGATAACCTAACGAAGAACTGAACTGCTATCATTACCATATCTCATCTCAATCTTATAAGTTGAACTTATAACAAAACAAATCAACAATAAAATGAATGAATCTTTCTCATCACCACCTCAATCCACACTTTCATCTATTTCTATATAGAGTTAATCAAGCTGACTTCAGTTACCAACATCATCAACAGCTATCTCGACCAACTCATCAATAACAAAGACAACATCATACACATTTTCTTAGTTCTAACGAAGAATCTTATTCGCCCGAAACGACTCCATGACTCGACGAAGCTGATTCTCTTCCTCCACAAACACATTTTCAGGCACCTGTAACCTCAACTCATACAGTTGATTGTTCTCAACTCCTAGAACAGACAAGTACCTCCTATCCCATTCCATACGCACCACCCTGTCTTGTGGCATAACAGCAAGCTCATTGTTACTAGCATACGACTTTATATTCGCCTCAACTTGATAGTACAATTTACCATCATCAGCAACTCTAGAAGAAGTGTTAAGAATACTAGATTCACGTTTAACACCGATTCTAGTTGACATAAACTCAGTAAGATACTGATTAAGAACTTTCTTCCCAGCTTCTTCCGGTGGACCCAAGTCTTCCACAGTCTTGAATTTCGAAGACGAAGGTGAAGAAACTTCAACAGAGATGTTTTCATCAAGAACATAAGGATCCCTGAAGAAAATATCAGCACCAGCACCTCGAACTTGAATCCAATTCGATGGGTAGTTGAACGAATAGCCATCAAAAGTGTCGACATATTCTCGGAACCTACGAGGAGGTGGTTGTTGTTGTTGCTGCTGCTCAGCTAATGATGTACCAATGTCGGAGAGAATGTAAGTTGAGAAGATTAAGGATATTGCTTTTCTCCTCGGAACTGTAAAAGCTTTAGCCGAATTGGGTGTGGGAATGGAACAGCGAGGGGAATGGAAGTGAAACGGTGTTAGAAAACTCGTCGTTGTGATTGTGTTGGCCATTGCGATTTGCAGAGTAAAGGCAAATGGATAGAATGGAAGGAACAAGAACAACAGGAGGAGCAACAACCGCACGCCACAATGTTATTCGTTTTCTTCTCAATCTATTTTTTTGTTTTCCCCCGTCCG
TARGET=902,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0410
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.006945
PRIMER_LEFT_SEQUENCE=CCCTGAAGAAAATATCAGCACC
PRIMER_RIGHT_SEQUENCE=CATTCTCTCCGACATTGGTACA
PRIMER_LEFT=790,22
PRIMER_RIGHT=953,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.0820
PRIMER_LEFT_1_PENALTY=0.075027
PRIMER_RIGHT_1_PENALTY=0.006945
PRIMER_LEFT_1_SEQUENCE=AATCCAATTCGATGGGTAGTTG
PRIMER_RIGHT_1_SEQUENCE=CATTCTCTCCGACATTGGTACA
PRIMER_LEFT_1=827,22
PRIMER_RIGHT_1=953,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=0.0850
PRIMER_LEFT_2_PENALTY=0.078029
PRIMER_RIGHT_2_PENALTY=0.006945
PRIMER_LEFT_2_SEQUENCE=CGAAGACGAAGGTGAAGAAACT
PRIMER_RIGHT_2_SEQUENCE=CATTCTCTCCGACATTGGTACA
PRIMER_LEFT_2=734,22
PRIMER_RIGHT_2=953,22
PRIMER_LEFT_2_TM=59.922
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.1081
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.073989
PRIMER_LEFT_3_SEQUENCE=CCCTGAAGAAAATATCAGCACC
PRIMER_RIGHT_3_SEQUENCE=CACAATCACAACGACGAGTTTT
PRIMER_LEFT_3=790,22
PRIMER_RIGHT_3=1085,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.1419
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.107763
PRIMER_LEFT_4_SEQUENCE=CCCTGAAGAAAATATCAGCACC
PRIMER_RIGHT_4_SEQUENCE=GCTTTTACAGTTCCGAGGAGAA
PRIMER_LEFT_4=790,22
PRIMER_RIGHT_4=1003,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.892
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_04448 Ps_contig_mira-and-tgicl-ass_franssen_33247
SEQUENCE=TTAAAATTAAACCATCATTATCATACAGTGCAGCTCTTAATTTAACCAAAATGTCCAAGTATACAGAACTAAGGGAACCACATACTCGACAAATGGACTTAAGGAAATGCCCAGTATCTACTAACCATCATGTTAAGGCCAAAAATTGCCCATTCATTATGCACAAGTGGCCAACATCAAAACAGTCTCGAAAGTGTAAGGATTTCTCTCTTCCTGAATCTCTCTCTTAATATTAACTACAATCAAAGAAATATCACGAAACACTCTGACACCTCAGAATTACGCTTTCAGTAGAGCACTGGCCACAACAAAATCAGAAGTCAAACTCTCCTGTTGCCTTGTTGAAGAGAATATCCTTGTTATTTATATGCATGATGCCATCCTTTAATGTGCATTTCCACCTGCTCTTAGTACGTGTCACCTTGTCAAACTGGGCCAAAACTAGATGATGTGTATTTTGATCATCCCCTTGGTCCAAGTCATCTAAATCATCATCATCGTTTTCATTTAATGGAGGCTCATCGTCGTCATCGTCATTTACCACCTCATTTTGAGCAACGAGAGCAGGGGTGCTTACTGGTGCTTCAGGAGGAGCTGGTGTATTTGCAACGTTGTAGTCTTCACTGTACACTCCACCATTATAGTATATATTTGGAGTTGAAAGCACATCATCCTCGTAAGGAATTGGTCCATCAAGTTGAGGAATCCTAGAAGTCAGTTTCTCCAAGTCTGCGGGCATTTTCCCTTTTGAATTAGTGTGTTGTGAACTGAAGGAGATGCTCCCTCCACATACCTCAATTTCAAAATCGCTGGATCCAGCATCACCAGCTCCATCTTGCTGAGGTATATACCCTCCGACATCATATGGTGGAGGCATATCATTGCGTTTACGCTTTCCACCAGGCACTGTGAAGAAATCTTGTGTCATAGGCTGATTAGAAGCTCCTCTATCTGCCTCTTCCCGTCCTTCCACATAAGCAACATTGACATCAAGTGGAGGCCTCTGATTCATCCAAGGAGAAGGAGGTTGCATGAATGGACTTGGTCTTCCACCTTTAACATCAGCATTTCCACCGGTTTCATTTCCAGAAGAAGAGTAGTCACTTGGTCCAGTAGGAATGTTATAATTTGGGGTCTCCCCAGCTCCCGGTAAAGGGGTTTGAATTGGAGTTTGCAATGGCGTAGGGGGGAAAAGTATTTCAGCAGTAGGAGTTTCATACTCCTCAGTTCCCTCATACGGCACATTCAGATCATGAACCGGAGTAATGGGACCACCAGGAGTCGGTCTATTAGCACCGGTAGACCTCTCAATAGGACCAAGCACGGCACCAGCTTGAATCATCTTCGTTTCCCAAATGGCTTGAAGCTCTCTGAGAACTTCATCTCCGGGACCACCGCCGTTGGTGACGAACTCATCTCGAACCTTGACCATGACATCTTCGATAACATCGATATAAACCTGACTTGTAGTTGAAGCCGCCATCAAGAATCGAGGGAAATTATGAGCGATTGATTCTCCGTTTTCGTCACGGATTGGAAAGGTTTTGAGAAAAAGAAATTGAAGAAAGTGTGAAGAAGAGGAAGAAGGTGTGTGTCTGGTTTTAGGGTTTGCGATGGATTGGGGAAGGAGGAGGGAGAAGGGGATATGAGAAAGAGAGAAAGAAACAAGAGGCGGACCAGAAAGGAAGAATAAAGAGAAGAATATAAATAGGTTTGCATCCGATTATAATCACCGACCCCCCA
TARGET=488,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1186
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.117817
PRIMER_LEFT_SEQUENCE=AAACTGGGCCAAAACTAGATGA
PRIMER_RIGHT_SEQUENCE=GATGATGTGCTTTCAACTCCAA
PRIMER_LEFT=427,22
PRIMER_RIGHT=672,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.118
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.1311
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=AAACTGGGCCAAAACTAGATGA
PRIMER_RIGHT_1_SEQUENCE=AAATACACCAGCTCCTCCTGAA
PRIMER_LEFT_1=427,22
PRIMER_RIGHT_1=604,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.1376
PRIMER_LEFT_2_PENALTY=0.019797
PRIMER_RIGHT_2_PENALTY=0.117817
PRIMER_LEFT_2_SEQUENCE=TATTTTGATCATCCCCTTGGTC
PRIMER_RIGHT_2_SEQUENCE=GATGATGTGCTTTCAACTCCAA
PRIMER_LEFT_2=453,22
PRIMER_RIGHT_2=672,22
PRIMER_LEFT_2_TM=60.020
PRIMER_RIGHT_2_TM=60.118
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.1416
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.140832
PRIMER_LEFT_3_SEQUENCE=AAACTGGGCCAAAACTAGATGA
PRIMER_RIGHT_3_SEQUENCE=TGAGGTGGTAAATGACGATGAC
PRIMER_LEFT_3=427,22
PRIMER_RIGHT_3=547,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=59.859
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=121
PRIMER_PAIR_PENALTY_4=0.1502
PRIMER_LEFT_4_PENALTY=0.019797
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=TATTTTGATCATCCCCTTGGTC
PRIMER_RIGHT_4_SEQUENCE=AAATACACCAGCTCCTCCTGAA
PRIMER_LEFT_4=453,22
PRIMER_RIGHT_4=604,22
PRIMER_LEFT_4_TM=60.020
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_04449 Ps_contig_mira-and-tgicl-ass_franssen_33250
SEQUENCE=GTTTCAATGAAACATTGTACGTGCTTCCAAGTTTCTTAYAATTTTCCTGTAGTTCGTGCTTGCAGATAATCGATGTCGATACAAACGAGGGGGTAGCTCAATATATAATTAACCAACCTCCTGATCTGTTATTTTCATATTATTGGAAAAATATACAAGTCCTAAAAATTTCCCCCAGCATTTACAGCTTATGGGAAATGACATTGAAAATGATGGAAAACATTCCCACCTGACAGTTAAAAGCTATTATTTCCTTGCGATTGATTATAAGCTCGACAACAAAAAAAGAAGTCAGGAGAAGAGGAATAGAAGGGTCTCCAGGCATCAGGGACATTACATCAGAAAAACAAAAAACAAACAATTACATTACATGCACTGTTTCTAAAATATTTCTTCTATGCTCTTTGCTCAACATATGAACCCGCAAGTCCGGAAGTTCCAAGAACCGTAACCTTTCCCCACTCAGAACCAAGGCCACTGCATCTCATTTTCAAGTGAATCATCTTTGTACCCAATTCCGACTCCACTTTCTTTCGCAGATAGTTCTCCTCAGGACCAAAAGCATTCAGATAGGTGGTGTAGCGATCAAAAATTGTATGCAGAGCTTTGTTCACATGATCAGGCAAAGGCTTTACATCCTCGCCGCTCAGGCCAATGCTGTCATCAATTTCCATTTTCAAGGTGACAAGAATCCCACCGAACTCTTCAACAGCCTGGGCGGCACGGAACACATTGGTCACAACCTCCTTACCAGCTTCATCTGCTGTACTTTTTGCCAACGCATGCTTAGCATCAGACACAACAGCATCTGAAATATCACTCCAATTTTCAGCCAACAAATCCTTGAAAACACTGTAAAGCTCAGCATCCTTAATCTCAGGCACATGACTCACATCTTCGCTATAAAATCGCAAGCTTCCTACGCCATTCGAGAAAGGAGACCTGCATTCCGAGGAGGGAGAGAGAGGATTGAGAAAGCGGGGGAAGAAAGGGTTCGAAGGTTGTTGGTGGTGGCGGCGGTGGTGGCGGTGAAGGCACGGGAAGAGTAAGATCTGGAAGAGTTGAGTGATCGGAATAAGGATCTCAATCCCATCATCGTCTTCATCTTTCGCAGTTTTGGTTTGGAAAAGCTCGATTGCTACTTCGATTTTGTAGTTTTTTTATGTCTGATTCTTCTGCTTAATTAATTAATTAAT
TARGET=1180,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04450 Ps_contig_mira-and-tgicl-ass_franssen_33253
SEQUENCE=GCAACATAAATAAGCTATTAAGTTAACTTTAAATTTAACTAAAGGACATTTGTCAAAATTCATACTCTTAACAAAAGTTTAAGAATTGCAAAGTAAAGTTATTAACATTTATCAACAAACTATTAAGAACAGTGATGTGTCTCTGTAAATTAATAATTCCTCATTATATGCGTAATTTGTACTTGAGCTCCTTACTTTCTTCCAAATATTTGAACAATGCAGATAGTGCAATAGTATCATTGTGTGTCTCTCTCTCAACCAACCATTCATCATAAAGCTTAACATTTACGTCCCTTTTCAAGGCAAGAACTTCATAATTTCGCCGTAGGTAATCAGCATACTTTTTAAGGGCGTCTTGTGCATATGGTCTGCCCACGGTCCTTACTGCAGCTGCATTTAGTAAAGCTTCCAACGAACCATGTTTTTGAATAAGTTTTAGAGCAGTTTTCCGACCAAAACTAGGGACTAGATGTTGGATACCAGGAACACCGTCAACTTCATCGCCTATAATAGATCTGAGGCTCAGATCAGATTGTGGGTCACAATTATACTGATCTCTGTAGTGTCTCATAGTGTAGAAGGACCACCTCTGTAACTCCGGCAAAGGCATAACTATTTGCACATTGTCGGAAATAAGTTGCTTAAAATCTTTATCAGGGGAGGCAATCACCACTCGAAACCCTTTTTTGAGAACTTGTTCGGCAAGTGTAGCTACCACATCATCCGCTTCATGGCCATCAACTTTTACAACAGGCACATTGCATTTTCGAAGAACATCACTGATAACTGGATGAAACCTTCCAACATAACCATCACCGCCACCACCACCAGTTGAAAGTTTTCTGGTGAATTTTATTCGATTGGCTTTGTACGAAGGAAGCAAGTTTCTCCGATATTCAGTTCCCCTTTCACCGTCAACAACAGCGATAACAGGGTGAGATTGAGTGATTTGAGGAGACAGAAAACGTGAAAGCCATCGAGCAAAATAATGCAAACTAGGGTTACTTCCTTCGTAACAAAGAGGACTCACGTCTAAAAAGAATACCTTTTTGGTTTTAGGGTTTGGGTTTGGGTTTCTGGTTTGCGTGACGGTGGTGGTTGGAGTGTTGTTTTGAGGTTTGTTGAAGGACAGTGAAGAAGGAGAAGAAGGAGAAAATGCAGAAGATGAAGAAGAAGAAGAATAGTTGAAGTTGGAAGGGTGAATGAAATTGAAAGAATGTGATGAAGATGATGATAATGTTTTCATCTTTCTCTTCTATCCTCAATTTTTTCTTTCTTTTCTC
TARGET=247,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7421
PRIMER_LEFT_PENALTY=1.736455
PRIMER_RIGHT_PENALTY=0.005674
PRIMER_LEFT_SEQUENCE=TTGAGCTCCTTACTTTCTTCCA
PRIMER_RIGHT_SEQUENCE=TAGTCCCTAGTTTTGGTCGGAA
PRIMER_LEFT=182,22
PRIMER_RIGHT=467,22
PRIMER_LEFT_TM=58.264
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=1.8004
PRIMER_LEFT_1_PENALTY=1.794685
PRIMER_RIGHT_1_PENALTY=0.005674
PRIMER_LEFT_1_SEQUENCE=CTTGAGCTCCTTACTTTCTTCCA
PRIMER_RIGHT_1_SEQUENCE=TAGTCCCTAGTTTTGGTCGGAA
PRIMER_LEFT_1=181,23
PRIMER_RIGHT_1=467,22
PRIMER_LEFT_1_TM=59.205
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=1.8384
PRIMER_LEFT_2_PENALTY=1.736455
PRIMER_RIGHT_2_PENALTY=0.101938
PRIMER_LEFT_2_SEQUENCE=TTGAGCTCCTTACTTTCTTCCA
PRIMER_RIGHT_2_SEQUENCE=GCGAAATTATGAAGTTCTTGCC
PRIMER_LEFT_2=182,22
PRIMER_RIGHT_2=322,22
PRIMER_LEFT_2_TM=58.264
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=141
PRIMER_PAIR_PENALTY_3=1.8384
PRIMER_LEFT_3_PENALTY=1.736455
PRIMER_RIGHT_3_PENALTY=0.101938
PRIMER_LEFT_3_SEQUENCE=TTGAGCTCCTTACTTTCTTCCA
PRIMER_RIGHT_3_SEQUENCE=GGCGAAATTATGAAGTTCTTGC
PRIMER_LEFT_3=182,22
PRIMER_RIGHT_3=323,22
PRIMER_LEFT_3_TM=58.264
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=142
PRIMER_PAIR_PENALTY_4=1.8384
PRIMER_LEFT_4_PENALTY=1.736455
PRIMER_RIGHT_4_PENALTY=0.101938
PRIMER_LEFT_4_SEQUENCE=TGAGCTCCTTACTTTCTTCCAA
PRIMER_RIGHT_4_SEQUENCE=GCGAAATTATGAAGTTCTTGCC
PRIMER_LEFT_4=183,22
PRIMER_RIGHT_4=322,22
PRIMER_LEFT_4_TM=58.264
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=140
=
PRIMER_SEQUENCE_ID=SSR_04451 Ps_contig_mira-and-tgicl-ass_franssen_33253
SEQUENCE=GCAACATAAATAAGCTATTAAGTTAACTTTAAATTTAACTAAAGGACATTTGTCAAAATTCATACTCTTAACAAAAGTTTAAGAATTGCAAAGTAAAGTTATTAACATTTATCAACAAACTATTAAGAACAGTGATGTGTCTCTGTAAATTAATAATTCCTCATTATATGCGTAATTTGTACTTGAGCTCCTTACTTTCTTCCAAATATTTGAACAATGCAGATAGTGCAATAGTATCATTGTGTGTCTCTCTCTCAACCAACCATTCATCATAAAGCTTAACATTTACGTCCCTTTTCAAGGCAAGAACTTCATAATTTCGCCGTAGGTAATCAGCATACTTTTTAAGGGCGTCTTGTGCATATGGTCTGCCCACGGTCCTTACTGCAGCTGCATTTAGTAAAGCTTCCAACGAACCATGTTTTTGAATAAGTTTTAGAGCAGTTTTCCGACCAAAACTAGGGACTAGATGTTGGATACCAGGAACACCGTCAACTTCATCGCCTATAATAGATCTGAGGCTCAGATCAGATTGTGGGTCACAATTATACTGATCTCTGTAGTGTCTCATAGTGTAGAAGGACCACCTCTGTAACTCCGGCAAAGGCATAACTATTTGCACATTGTCGGAAATAAGTTGCTTAAAATCTTTATCAGGGGAGGCAATCACCACTCGAAACCCTTTTTTGAGAACTTGTTCGGCAAGTGTAGCTACCACATCATCCGCTTCATGGCCATCAACTTTTACAACAGGCACATTGCATTTTCGAAGAACATCACTGATAACTGGATGAAACCTTCCAACATAACCATCACCGCCACCACCACCAGTTGAAAGTTTTCTGGTGAATTTTATTCGATTGGCTTTGTACGAAGGAAGCAAGTTTCTCCGATATTCAGTTCCCCTTTCACCGTCAACAACAGCGATAACAGGGTGAGATTGAGTGATTTGAGGAGACAGAAAACGTGAAAGCCATCGAGCAAAATAATGCAAACTAGGGTTACTTCCTTCGTAACAAAGAGGACTCACGTCTAAAAAGAATACCTTTTTGGTTTTAGGGTTTGGGTTTGGGTTTCTGGTTTGCGTGACGGTGGTGGTTGGAGTGTTGTTTTGAGGTTTGTTGAAGGACAGTGAAGAAGGAGAAGAAGGAGAAAATGCAGAAGATGAAGAAGAAGAAGAATAGTTGAAGTTGGAAGGGTGAATGAAATTGAAAGAATGTGATGAAGATGATGATAATGTTTTCATCTTTCTCTTCTATCCTCAATTTTTTCTTTCTTTTCTC
TARGET=819,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0425
PRIMER_LEFT_PENALTY=0.016950
PRIMER_RIGHT_PENALTY=0.025566
PRIMER_LEFT_SEQUENCE=ACCTTCCAACATAACCATCACC
PRIMER_RIGHT_SEQUENCE=ATCTCACCCTGTTATCGCTGTT
PRIMER_LEFT=795,22
PRIMER_RIGHT=940,22
PRIMER_LEFT_TM=59.983
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=0.0425
PRIMER_LEFT_1_PENALTY=0.016950
PRIMER_RIGHT_1_PENALTY=0.025566
PRIMER_LEFT_1_SEQUENCE=ACCTTCCAACATAACCATCACC
PRIMER_RIGHT_1_SEQUENCE=AATCTCACCCTGTTATCGCTGT
PRIMER_LEFT_1=795,22
PRIMER_RIGHT_1=941,22
PRIMER_LEFT_1_TM=59.983
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.0579
PRIMER_LEFT_2_PENALTY=0.032347
PRIMER_RIGHT_2_PENALTY=0.025566
PRIMER_LEFT_2_SEQUENCE=CCATCAACTTTTACAACAGGCA
PRIMER_RIGHT_2_SEQUENCE=AATCTCACCCTGTTATCGCTGT
PRIMER_LEFT_2=734,22
PRIMER_RIGHT_2=941,22
PRIMER_LEFT_2_TM=60.032
PRIMER_RIGHT_2_TM=60.026
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.0886
PRIMER_LEFT_3_PENALTY=0.016950
PRIMER_RIGHT_3_PENALTY=0.071684
PRIMER_LEFT_3_SEQUENCE=ACCTTCCAACATAACCATCACC
PRIMER_RIGHT_3_SEQUENCE=GCTTTCACGTTTTCTGTCTCCT
PRIMER_LEFT_3=795,22
PRIMER_RIGHT_3=973,22
PRIMER_LEFT_3_TM=59.983
PRIMER_RIGHT_3_TM=59.928
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=179
PRIMER_PAIR_PENALTY_4=0.1040
PRIMER_LEFT_4_PENALTY=0.032347
PRIMER_RIGHT_4_PENALTY=0.071684
PRIMER_LEFT_4_SEQUENCE=CCATCAACTTTTACAACAGGCA
PRIMER_RIGHT_4_SEQUENCE=GCTTTCACGTTTTCTGTCTCCT
PRIMER_LEFT_4=734,22
PRIMER_RIGHT_4=973,22
PRIMER_LEFT_4_TM=60.032
PRIMER_RIGHT_4_TM=59.928
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_04452 Ps_contig_mira-and-tgicl-ass_franssen_33253
SEQUENCE=GCAACATAAATAAGCTATTAAGTTAACTTTAAATTTAACTAAAGGACATTTGTCAAAATTCATACTCTTAACAAAAGTTTAAGAATTGCAAAGTAAAGTTATTAACATTTATCAACAAACTATTAAGAACAGTGATGTGTCTCTGTAAATTAATAATTCCTCATTATATGCGTAATTTGTACTTGAGCTCCTTACTTTCTTCCAAATATTTGAACAATGCAGATAGTGCAATAGTATCATTGTGTGTCTCTCTCTCAACCAACCATTCATCATAAAGCTTAACATTTACGTCCCTTTTCAAGGCAAGAACTTCATAATTTCGCCGTAGGTAATCAGCATACTTTTTAAGGGCGTCTTGTGCATATGGTCTGCCCACGGTCCTTACTGCAGCTGCATTTAGTAAAGCTTCCAACGAACCATGTTTTTGAATAAGTTTTAGAGCAGTTTTCCGACCAAAACTAGGGACTAGATGTTGGATACCAGGAACACCGTCAACTTCATCGCCTATAATAGATCTGAGGCTCAGATCAGATTGTGGGTCACAATTATACTGATCTCTGTAGTGTCTCATAGTGTAGAAGGACCACCTCTGTAACTCCGGCAAAGGCATAACTATTTGCACATTGTCGGAAATAAGTTGCTTAAAATCTTTATCAGGGGAGGCAATCACCACTCGAAACCCTTTTTTGAGAACTTGTTCGGCAAGTGTAGCTACCACATCATCCGCTTCATGGCCATCAACTTTTACAACAGGCACATTGCATTTTCGAAGAACATCACTGATAACTGGATGAAACCTTCCAACATAACCATCACCGCCACCACCACCAGTTGAAAGTTTTCTGGTGAATTTTATTCGATTGGCTTTGTACGAAGGAAGCAAGTTTCTCCGATATTCAGTTCCCCTTTCACCGTCAACAACAGCGATAACAGGGTGAGATTGAGTGATTTGAGGAGACAGAAAACGTGAAAGCCATCGAGCAAAATAATGCAAACTAGGGTTACTTCCTTCGTAACAAAGAGGACTCACGTCTAAAAAGAATACCTTTTTGGTTTTAGGGTTTGGGTTTGGGTTTCTGGTTTGCGTGACGGTGGTGGTTGGAGTGTTGTTTTGAGGTTTGTTGAAGGACAGTGAAGAAGGAGAAGAAGGAGAAAATGCAGAAGATGAAGAAGAAGAAGAATAGTTGAAGTTGGAAGGGTGAATGAAATTGAAAGAATGTGATGAAGATGATGATAATGTTTTCATCTTTCTCTTCTATCCTCAATTTTTTCTTTCTTTTCTC
TARGET=1167,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3577
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.332137
PRIMER_LEFT_SEQUENCE=ACAGCGATAACAGGGTGAGATT
PRIMER_RIGHT_SEQUENCE=TTTCATTCACCCTTCCAACTTC
PRIMER_LEFT=920,22
PRIMER_RIGHT=1207,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=60.332
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.3577
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.332137
PRIMER_LEFT_1_SEQUENCE=AACAGCGATAACAGGGTGAGAT
PRIMER_RIGHT_1_SEQUENCE=TTTCATTCACCCTTCCAACTTC
PRIMER_LEFT_1=919,22
PRIMER_RIGHT_1=1207,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=60.332
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=289
PRIMER_PAIR_PENALTY_2=0.4038
PRIMER_LEFT_2_PENALTY=0.071684
PRIMER_RIGHT_2_PENALTY=0.332137
PRIMER_LEFT_2_SEQUENCE=AGGAGACAGAAAACGTGAAAGC
PRIMER_RIGHT_2_SEQUENCE=TTTCATTCACCCTTCCAACTTC
PRIMER_LEFT_2=952,22
PRIMER_RIGHT_2=1207,22
PRIMER_LEFT_2_TM=59.928
PRIMER_RIGHT_2_TM=60.332
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.4408
PRIMER_LEFT_3_PENALTY=0.108684
PRIMER_RIGHT_3_PENALTY=0.332137
PRIMER_LEFT_3_SEQUENCE=TGAGGAGACAGAAAACGTGAAA
PRIMER_RIGHT_3_SEQUENCE=TTTCATTCACCCTTCCAACTTC
PRIMER_LEFT_3=950,22
PRIMER_RIGHT_3=1207,22
PRIMER_LEFT_3_TM=59.891
PRIMER_RIGHT_3_TM=60.332
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=0.4408
PRIMER_LEFT_4_PENALTY=0.108684
PRIMER_RIGHT_4_PENALTY=0.332137
PRIMER_LEFT_4_SEQUENCE=TTGAGGAGACAGAAAACGTGAA
PRIMER_RIGHT_4_SEQUENCE=TTTCATTCACCCTTCCAACTTC
PRIMER_LEFT_4=949,22
PRIMER_RIGHT_4=1207,22
PRIMER_LEFT_4_TM=59.891
PRIMER_RIGHT_4_TM=60.332
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=259
=
PRIMER_SEQUENCE_ID=SSR_04453 Ps_contig_mira-and-tgicl-ass_franssen_33257
SEQUENCE=CTGTTCTGGGCTACAAATATGCATTATAGCAAACTAATTTGGAGAAAGCGAATTCACATGGTTATACATAATTCAGAACAATGAAACAAATTTTCACATGCATGTAATGTGGTTCTTTATATCAGAGAATATGAAGTAGGGACATCATTACTTTTAGGACGAAAAACATGAATAAGTAAATAAGACATTGGAATTAGAAACCACGGTTTCTACCTGAAGATTTTATCTTAAGCAGCAGCAGCTTCTGTTACATTTCCTATTGTTTTCCGGTCAAAGATTTCAATCCAGTATCCATCGGGATCCTTAATAAATGCTATCCCTTTCATTTTTCCATCATCTGGTTTCTTAACAAACTCAACCCCCAGATTCTGAAATCTTTCACATGCTTTGTAGGTGTCATCAACAGTTATGCCAATATGTCCAAAACCACGAGGATCGGAATTGCCATTGTGGTGCCCTTTGAACTCTGGATCACTTTCGGTGCCCCAATTATGTGTCAATTCAACTGTAGCCTTTTGAGCAAAGGTCCAAACTGTTCTATCAACAGGGTTAGTTGGAGCTTCTGATGTATCCTCATACCCCATAAAGTACAAGCTGAACTTCAACTCGGGAAAATCCAGYCTCTTGAGCAAAGACATGCCCAAAATGCGGGAGTAAAAATCAAGGCTAACTTTGGGGTCTTTGATTCTATACATAGTTTGTTGCATAAAGTAGCCCTTAGTAGCTTCATCTATAGTTGCATGGAGACCGGGGTTGTTAGCCGGCGATTCCTTCGGCTCAGCAGCAGCCATTGAGAAAAAACGAAACCGAGCCGGTTGCTTGGGATGATTTTGATGAAGGTGAAGATGAGAGTCTCGGCGAGAATAGAGAGAGTGGCGAACCGCTCCCCG
TARGET=231,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04454 Ps_contig_mira-and-tgicl-ass_franssen_33260
SEQUENCE=AAATTAGAACAACTTTTCTNAATTAAGCTGTATATATTTATTCTTCGCACCAAGATTACTGAAGCCAGCAAACTCAAAGATATTTAACAATAATACACAATAACATAAATGAATTGTGGTAATGAATTACCAAAAAGTTGCAAACTATCAAAGATAAAAAATAAAAAGGTATCAAATGTATACTCCCCTCAATACTGTCTTTACCATATACTAGTAGATCTAACTGATATTGTTGTTTTTACAGCTATGAGCTACAAGGTACACACCAAATATGCATAAGATATGATTTCAATTCAACAGGGAAACTCTACTATCATTGCTTGCAATTGAACATTCATGAATCTGCAATGGGGAAAACAGATCCAATTTGATTGCCTAAATGGTAGCACTTGATATTAACTGCATATGGACCAAGCTGAATGTGGTTATCTTCCTGACCAGCGAAGAAACACAGGGTATACAAAGCAATTTCAAATTCAGGGCTCACTCCGACCAAAGTACTTGATACAGATTTCAGAACACCATTCCATTCAAATTGAATTGTCAGAAGCTGGGTTTCAGAGTCAGGAGTTTCACCCCGTCGACGGGGAAAAATATAGCCTTGATAATCAACTTTTCCATTTGCTTCTTCATGGTAAAACTGCAGCCAGTTATGAAATCCAGAAACTTCACTGCTTTGTTTGATTTCTCCAACAAAAACATGCTCAAAAGCGGAAGAGGAACCAGAAGTTCCACATCGGCTATAAAGATCAAACCACAAACTGGTCAACAATCTTTTGAAATCTTGGGAACTCCCTGATGTGATTCCTTTGGATGCAAGAAACTTGTGAAGATACTTTATTGGGGCTGTTCTACTAATTTCTTCTATGAAAGAAGCTTGCTCTTGTTTCTCTTCAGATGTGACAACCTCCTTAGATCCTTGATGTGGATTGTAATTATCTAACAGTGAAAGAAATCGAGCATAAGTAGGCTTTCGGAATACATCATCACTAACCCATGTAAACAAGCTTCCCTGCGCCATATCATCCTTTTGATAAACTTTTTTCCCTTCACCACAGTCAATTTCATAGTCTTTGCCAGGTATCAAACGATTTAAATCCAGTTCCCAGAGTTTTTCACAAGCCCTTGATAGATCAGCCAGTTCATCTTCTGAGGGCTCTACTCTGGCACCAACTTCAACTTCATTGGAGTATTGTTGCTCATCAGAAGGAAGTTTATAGCTGTTCCAGTTATCCTTTGGAACCTTGTGTGTCTGTCTTGAAGGTTTGCCCACAGTCTGCCATCCATCGTTGTTATCTTCATCTGACTTATTAGGCCTTTGACGGTTACTATCCTTTTGCCATGGTTCTACTTCCTCCTTCTTGTAGTTGTGAGGTCTATCGGAGATTCTAGAACCTTCGGATTGCCACTCCTCCTGCTTCTGAGGCTGTGGACGGTGATCGGAATCCTGATCTCCGGAAACGACCTCCGCCCAAGAGGATCTGGATCGTTCGTCTCGTGATTGACGATCATCGTCGTTATCTTGATCGCGATCGTTGTTGCCGCCGAGTGCTACGTCGATCAAGCCCTTGATGAGACCATCCATGATAAAGAGAGTGTGTTGTGTTGTGTTGTTTGTGAGTGTTACTCTCCCCGANTCTGAAATGAGCGATGAAGTAAATTAGTACCCCGA
TARGET=1597,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1017
PRIMER_LEFT_PENALTY=0.772991
PRIMER_RIGHT_PENALTY=0.328676
PRIMER_LEFT_SEQUENCE=GATTCTAGAACCTTCGGATTGC
PRIMER_RIGHT_SEQUENCE=TTTCAGANTCGGGGAGAGTAAC
PRIMER_LEFT=1384,22
PRIMER_RIGHT=1643,22
PRIMER_LEFT_TM=59.227
PRIMER_RIGHT_TM=59.671
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.75
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=5.75
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=1.1173
PRIMER_LEFT_1_PENALTY=0.788621
PRIMER_RIGHT_1_PENALTY=0.328676
PRIMER_LEFT_1_SEQUENCE=ATTCTAGAACCTTCGGATTGCC
PRIMER_RIGHT_1_SEQUENCE=TTTCAGANTCGGGGAGAGTAAC
PRIMER_LEFT_1=1385,22
PRIMER_RIGHT_1=1643,22
PRIMER_LEFT_1_TM=60.789
PRIMER_RIGHT_1_TM=59.671
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.75
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.75
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=1.2583
PRIMER_LEFT_2_PENALTY=0.929660
PRIMER_RIGHT_2_PENALTY=0.328676
PRIMER_LEFT_2_SEQUENCE=ATCATCGTCGTTATCTTGATCG
PRIMER_RIGHT_2_SEQUENCE=TTTCAGANTCGGGGAGAGTAAC
PRIMER_LEFT_2=1507,22
PRIMER_RIGHT_2=1643,22
PRIMER_LEFT_2_TM=59.070
PRIMER_RIGHT_2_TM=59.671
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.75
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.3000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=1.3040
PRIMER_LEFT_3_PENALTY=0.772991
PRIMER_RIGHT_3_PENALTY=0.530964
PRIMER_LEFT_3_SEQUENCE=GATTCTAGAACCTTCGGATTGC
PRIMER_RIGHT_3_SEQUENCE=CATTTCAGANTCGGGGAGAGTA
PRIMER_LEFT_3=1384,22
PRIMER_RIGHT_3=1645,22
PRIMER_LEFT_3_TM=59.227
PRIMER_RIGHT_3_TM=60.531
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.75
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.75
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=1.3196
PRIMER_LEFT_4_PENALTY=0.788621
PRIMER_RIGHT_4_PENALTY=0.530964
PRIMER_LEFT_4_SEQUENCE=ATTCTAGAACCTTCGGATTGCC
PRIMER_RIGHT_4_SEQUENCE=CATTTCAGANTCGGGGAGAGTA
PRIMER_LEFT_4=1385,22
PRIMER_RIGHT_4=1645,22
PRIMER_LEFT_4_TM=60.789
PRIMER_RIGHT_4_TM=60.531
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.75
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=261
=
PRIMER_SEQUENCE_ID=SSR_04455 Ps_contig_mira-and-tgicl-ass_franssen_33272
SEQUENCE=GTCGGGGATACATCCTATGACTTTTGTTCTTTGTCCTTCACTTTCGACCTTCTACATTTGTATTCTTTTTATAAACAAACCAACGCACACTTCACAACTNATTCACGCTTTCATTATTCTCTTCCACTACAATGCCTTCCCAAACTTTTCTCACTTTCTTCTTCTTCCTTCTCCTTTCTTTCAACACCATCACTTCCGATTTTGTTCGTCCTATAGATCGCAAATTGTTTGACCTACACAAGAAAGAAAAGCTAAGTCATTTCAAATTCTATTGGCATGACATTGTGAGTGGAAAAAACCCATCTTCAATCACGGTTGTTCCACCACCATTGAAACTAAACACAACCACTGCTTTTGGTTCAGTTCGCATGATTGATAACCCTTTAACATTAGGACCAGAATTGAGTTCCAAACTTGTTGGAAAATCACAAGGCTTCTATGCTTCTGCATGTCAAGATCAGATTGGTTTGCTTATGGCTATGAATTTAGCTTTCATTGAAGGAAAGTATAATGGAAGTAGTATAACTATCTTGGGGAGGAATTCTGTTTATGATAAGGTTAGGGAGATGCCTGTGATTGGTGGGAGTGGACTCTTCAGATTTGCTAGAGGATATGCTCAAGCTAATACTTACTCGTTTGATCTTAAATCTGGAGATGCCGTTGTTGAGTACAATGTTTATGTTTTTCACTATTGATTYTTAGGATTTGTGTTTGTGTTAGCTTTTTCATCAGAAGATTATGTATTTGGTTCTATGGTGTTGGATTTGGATTTACTGAGATTATTAATTTAATAAACTTGTAGTTGTTGTAAGCAACTGATATGGTTATTACATTGACATATCAAAGCCTTTTTCTATTTGAGGGTTTGCTTGTGAAGCTTGGTTTTTCCTTTTTTTGGAATCCATATTTGTCCTCTTTTATCTATTGTAAATTAAATACAACTTAGTTAGGTAGAAATGATGAGATTGTATTTTAAAAAGTCTTCTTGAAC
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04456 Ps_contig_mira-and-tgicl-ass_franssen_33279
SEQUENCE=AATTTTTCCTAACTAATTTGATTTTTAAATTTGTGCCCACATCACATTCCCAAAAATAAAGGGTTCTTTAAAAGAAAATGAAGATGATAAATCTGATTTTTGAACAACCAAACGTAGTCTGATTCCTGCTGTCATCATACCTGACCACTAATAATGCCAGTCATTCCATTAAAGAAAATCTAGAGAACTGATTCCCAAAGTTACATGTTGAAATCAAAACGAAGGAGCTGGATATACAATGAGGCAGAGACTATCACCAAATATTGGCAATGCTTTTATAATATGGGATAATATCTCTCAACTTGCACAAATTTTCTTCAATTTGAAATGAAAAATCGTTCATTCTTACAAGTTGCTTCCAGAAGCAGAGACCTCTGTACTGCAAACAGGGCAAACCTTGTTTATTTTCAACCAGTTGTTAATGCACTCAGGATGGTACAGATGTTTGCAGGAAAGAACTGTCAAGGACTCATCATCCTCATAATCCACCCGGCAAATGACACACGAATCATTGCTCCCATGCTGATCACTCCCCGTCTTATAGTTGATTGAAGGTAAACAGGCAATAGTATCAGTCGAAAGGCCTCTGCTCTCAGTTCCTACTACCTCACTCAATGCCAGGAGTTCCTCATATGAAAGTTCATCTGGATCAACATCCTCCCAAGCATCCTGAGAATTAGCACCATGCTCCTCCATGTCCTCAGCATCCTGATCATGTATCCCAGCAAGTGCCAACAGTCTAGCTGCCATATCCCTCTCTTCAGCCCCCTGTAATGCTCTTGCATAGGCTTCATCACTTGAAAACAGCTCGGGATCAAATTCAATGGTGGGGTTATCATGTTCTCCAGCACTAGCATGAGCATGAACATCAAATACATCTTCATCTTCATATCCCTCCTCATTATCGTCATCCTCATCCTCATCACCATCATCATCATCCTCATCTACATCAGTGCCATCATGAAGATCATCAAAATCATCTCCATCATCATGCAAATAACTCTCACCCTCCCAACTTCCATAGTCACTTCCATCATTATTCATTCTCAGCATCATGTATGCCCTTTCCTGCTCTTGAAGGGTTCGAGCGAGGGCGAGGTCAGCATCGACTTGGCTGAGATTAGTGAAAGGCGTTCTGGAAGGTTGTTGAGCGGCGGTTTCAGATCCAGTAGGTTGTTGTTGTTCCCCTTTGTCTTCGTCTTCTTCTTCTTTCTCTTCCATTTTTTCTGGTATGTTTTCCTCGCCGTTCAAATTAGAATGTTCTTCTTCGTGGATATGGTTGTTGGATTTGACTCCTCCGCCGCCGCCGCCTGAACCTAGTTTGGCCGTGTTGACGGTGGCGTTGACGGTGGTGGTGGTGGCGTTGGTGGTGTCGTTCTCCATTGGAATTAGGAAGAAGAAGAAAAAGAGAGTGAATAGAAAACAAGAATGATCGAATTCGAATCNGACGAATCAACCT
TARGET=927,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1559
PRIMER_LEFT_PENALTY=0.075031
PRIMER_RIGHT_PENALTY=0.080887
PRIMER_LEFT_SEQUENCE=CCTCCTCATTATCGTCATCCTC
PRIMER_RIGHT_SEQUENCE=AACGCCTTTCACTAATCTCAGC
PRIMER_LEFT=893,22
PRIMER_RIGHT=1134,22
PRIMER_LEFT_TM=59.925
PRIMER_RIGHT_TM=59.919
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.1764
PRIMER_LEFT_1_PENALTY=0.095523
PRIMER_RIGHT_1_PENALTY=0.080887
PRIMER_LEFT_1_SEQUENCE=TCATGTTCTCCAGCACTAGCAT
PRIMER_RIGHT_1_SEQUENCE=AACGCCTTTCACTAATCTCAGC
PRIMER_LEFT_1=835,22
PRIMER_RIGHT_1=1134,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=59.919
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.1764
PRIMER_LEFT_2_PENALTY=0.095523
PRIMER_RIGHT_2_PENALTY=0.080887
PRIMER_LEFT_2_SEQUENCE=ATGTTCTCCAGCACTAGCATGA
PRIMER_RIGHT_2_SEQUENCE=AACGCCTTTCACTAATCTCAGC
PRIMER_LEFT_2=837,22
PRIMER_RIGHT_2=1134,22
PRIMER_LEFT_2_TM=59.904
PRIMER_RIGHT_2_TM=59.919
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=0.1894
PRIMER_LEFT_3_PENALTY=0.053348
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=ATGGTGGGGTTATCATGTTCTC
PRIMER_RIGHT_3_SEQUENCE=GGAAGTGACTATGGAAGTTGGG
PRIMER_LEFT_3=823,22
PRIMER_RIGHT_3=1031,22
PRIMER_LEFT_3_TM=59.947
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.2041
PRIMER_LEFT_4_PENALTY=0.075031
PRIMER_RIGHT_4_PENALTY=0.129103
PRIMER_LEFT_4_SEQUENCE=CCTCCTCATTATCGTCATCCTC
PRIMER_RIGHT_4_SEQUENCE=TTCCAGAACGCCTTTCACTAAT
PRIMER_LEFT_4=893,22
PRIMER_RIGHT_4=1140,22
PRIMER_LEFT_4_TM=59.925
PRIMER_RIGHT_4_TM=60.129
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_04457 Ps_contig_mira-and-tgicl-ass_franssen_33279
SEQUENCE=AATTTTTCCTAACTAATTTGATTTTTAAATTTGTGCCCACATCACATTCCCAAAAATAAAGGGTTCTTTAAAAGAAAATGAAGATGATAAATCTGATTTTTGAACAACCAAACGTAGTCTGATTCCTGCTGTCATCATACCTGACCACTAATAATGCCAGTCATTCCATTAAAGAAAATCTAGAGAACTGATTCCCAAAGTTACATGTTGAAATCAAAACGAAGGAGCTGGATATACAATGAGGCAGAGACTATCACCAAATATTGGCAATGCTTTTATAATATGGGATAATATCTCTCAACTTGCACAAATTTTCTTCAATTTGAAATGAAAAATCGTTCATTCTTACAAGTTGCTTCCAGAAGCAGAGACCTCTGTACTGCAAACAGGGCAAACCTTGTTTATTTTCAACCAGTTGTTAATGCACTCAGGATGGTACAGATGTTTGCAGGAAAGAACTGTCAAGGACTCATCATCCTCATAATCCACCCGGCAAATGACACACGAATCATTGCTCCCATGCTGATCACTCCCCGTCTTATAGTTGATTGAAGGTAAACAGGCAATAGTATCAGTCGAAAGGCCTCTGCTCTCAGTTCCTACTACCTCACTCAATGCCAGGAGTTCCTCATATGAAAGTTCATCTGGATCAACATCCTCCCAAGCATCCTGAGAATTAGCACCATGCTCCTCCATGTCCTCAGCATCCTGATCATGTATCCCAGCAAGTGCCAACAGTCTAGCTGCCATATCCCTCTCTTCAGCCCCCTGTAATGCTCTTGCATAGGCTTCATCACTTGAAAACAGCTCGGGATCAAATTCAATGGTGGGGTTATCATGTTCTCCAGCACTAGCATGAGCATGAACATCAAATACATCTTCATCTTCATATCCCTCCTCATTATCGTCATCCTCATCCTCATCACCATCATCATCATCCTCATCTACATCAGTGCCATCATGAAGATCATCAAAATCATCTCCATCATCATGCAAATAACTCTCACCCTCCCAACTTCCATAGTCACTTCCATCATTATTCATTCTCAGCATCATGTATGCCCTTTCCTGCTCTTGAAGGGTTCGAGCGAGGGCGAGGTCAGCATCGACTTGGCTGAGATTAGTGAAAGGCGTTCTGGAAGGTTGTTGAGCGGCGGTTTCAGATCCAGTAGGTTGTTGTTGTTCCCCTTTGTCTTCGTCTTCTTCTTCTTTCTCTTCCATTTTTTCTGGTATGTTTTCCTCGCCGTTCAAATTAGAATGTTCTTCTTCGTGGATATGGTTGTTGGATTTGACTCCTCCGCCGCCGCCGCCTGAACCTAGTTTGGCCGTGTTGACGGTGGCGTTGACGGTGGTGGTGGTGGCGTTGGTGGTGTCGTTCTCCATTGGAATTAGGAAGAAGAAGAAAAAGAGAGTGAATAGAAAACAAGAATGATCGAATTCGAATCNGACGAATCAACCT
TARGET=1173,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1544
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.139129
PRIMER_LEFT_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_SEQUENCE=CAACAACCATATCCACGAAGAA
PRIMER_LEFT=1105,22
PRIMER_RIGHT=1284,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.861
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=0.1799
PRIMER_LEFT_1_PENALTY=0.015253
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_1_SEQUENCE=CCTAATTCCAATGGAGAACGAC
PRIMER_LEFT_1=1105,22
PRIMER_RIGHT_1=1392,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.2751
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.139129
PRIMER_LEFT_2_SEQUENCE=CCCAACTTCCATAGTCACTTCC
PRIMER_RIGHT_2_SEQUENCE=CAACAACCATATCCACGAAGAA
PRIMER_LEFT_2=1010,22
PRIMER_RIGHT_2=1284,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.861
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.2803
PRIMER_LEFT_3_PENALTY=0.015253
PRIMER_RIGHT_3_PENALTY=0.265053
PRIMER_LEFT_3_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_3_SEQUENCE=AGGAGTCAAATCCAACAACCAT
PRIMER_LEFT_3=1105,22
PRIMER_RIGHT_3=1296,22
PRIMER_LEFT_3_TM=60.015
PRIMER_RIGHT_3_TM=59.735
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=0.3199
PRIMER_LEFT_4_PENALTY=0.015253
PRIMER_RIGHT_4_PENALTY=0.304643
PRIMER_LEFT_4_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_4_SEQUENCE=TTCCTAATTCCAATGGAGAACG
PRIMER_LEFT_4=1105,22
PRIMER_RIGHT_4=1394,22
PRIMER_LEFT_4_TM=60.015
PRIMER_RIGHT_4_TM=60.305
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_04458 Ps_contig_mira-and-tgicl-ass_franssen_33279
SEQUENCE=AATTTTTCCTAACTAATTTGATTTTTAAATTTGTGCCCACATCACATTCCCAAAAATAAAGGGTTCTTTAAAAGAAAATGAAGATGATAAATCTGATTTTTGAACAACCAAACGTAGTCTGATTCCTGCTGTCATCATACCTGACCACTAATAATGCCAGTCATTCCATTAAAGAAAATCTAGAGAACTGATTCCCAAAGTTACATGTTGAAATCAAAACGAAGGAGCTGGATATACAATGAGGCAGAGACTATCACCAAATATTGGCAATGCTTTTATAATATGGGATAATATCTCTCAACTTGCACAAATTTTCTTCAATTTGAAATGAAAAATCGTTCATTCTTACAAGTTGCTTCCAGAAGCAGAGACCTCTGTACTGCAAACAGGGCAAACCTTGTTTATTTTCAACCAGTTGTTAATGCACTCAGGATGGTACAGATGTTTGCAGGAAAGAACTGTCAAGGACTCATCATCCTCATAATCCACCCGGCAAATGACACACGAATCATTGCTCCCATGCTGATCACTCCCCGTCTTATAGTTGATTGAAGGTAAACAGGCAATAGTATCAGTCGAAAGGCCTCTGCTCTCAGTTCCTACTACCTCACTCAATGCCAGGAGTTCCTCATATGAAAGTTCATCTGGATCAACATCCTCCCAAGCATCCTGAGAATTAGCACCATGCTCCTCCATGTCCTCAGCATCCTGATCATGTATCCCAGCAAGTGCCAACAGTCTAGCTGCCATATCCCTCTCTTCAGCCCCCTGTAATGCTCTTGCATAGGCTTCATCACTTGAAAACAGCTCGGGATCAAATTCAATGGTGGGGTTATCATGTTCTCCAGCACTAGCATGAGCATGAACATCAAATACATCTTCATCTTCATATCCCTCCTCATTATCGTCATCCTCATCCTCATCACCATCATCATCATCCTCATCTACATCAGTGCCATCATGAAGATCATCAAAATCATCTCCATCATCATGCAAATAACTCTCACCCTCCCAACTTCCATAGTCACTTCCATCATTATTCATTCTCAGCATCATGTATGCCCTTTCCTGCTCTTGAAGGGTTCGAGCGAGGGCGAGGTCAGCATCGACTTGGCTGAGATTAGTGAAAGGCGTTCTGGAAGGTTGTTGAGCGGCGGTTTCAGATCCAGTAGGTTGTTGTTGTTCCCCTTTGTCTTCGTCTTCTTCTTCTTTCTCTTCCATTTTTTCTGGTATGTTTTCCTCGCCGTTCAAATTAGAATGTTCTTCTTCGTGGATATGGTTGTTGGATTTGACTCCTCCGCCGCCGCCGCCTGAACCTAGTTTGGCCGTGTTGACGGTGGCGTTGACGGTGGTGGTGGTGGCGTTGGTGGTGTCGTTCTCCATTGGAATTAGGAAGAAGAAGAAAAAGAGAGTGAATAGAAAACAAGAATGATCGAATTCGAATCNGACGAATCAACCT
TARGET=1199,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1544
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.139129
PRIMER_LEFT_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_SEQUENCE=CAACAACCATATCCACGAAGAA
PRIMER_LEFT=1105,22
PRIMER_RIGHT=1284,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.861
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=0.1799
PRIMER_LEFT_1_PENALTY=0.015253
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_1_SEQUENCE=CCTAATTCCAATGGAGAACGAC
PRIMER_LEFT_1=1105,22
PRIMER_RIGHT_1=1392,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.2751
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.139129
PRIMER_LEFT_2_SEQUENCE=CCCAACTTCCATAGTCACTTCC
PRIMER_RIGHT_2_SEQUENCE=CAACAACCATATCCACGAAGAA
PRIMER_LEFT_2=1010,22
PRIMER_RIGHT_2=1284,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.861
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.2803
PRIMER_LEFT_3_PENALTY=0.015253
PRIMER_RIGHT_3_PENALTY=0.265053
PRIMER_LEFT_3_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_3_SEQUENCE=AGGAGTCAAATCCAACAACCAT
PRIMER_LEFT_3=1105,22
PRIMER_RIGHT_3=1296,22
PRIMER_LEFT_3_TM=60.015
PRIMER_RIGHT_3_TM=59.735
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=0.3199
PRIMER_LEFT_4_PENALTY=0.015253
PRIMER_RIGHT_4_PENALTY=0.304643
PRIMER_LEFT_4_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_4_SEQUENCE=TTCCTAATTCCAATGGAGAACG
PRIMER_LEFT_4=1105,22
PRIMER_RIGHT_4=1394,22
PRIMER_LEFT_4_TM=60.015
PRIMER_RIGHT_4_TM=60.305
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_04459 Ps_contig_mira-and-tgicl-ass_franssen_33279
SEQUENCE=AATTTTTCCTAACTAATTTGATTTTTAAATTTGTGCCCACATCACATTCCCAAAAATAAAGGGTTCTTTAAAAGAAAATGAAGATGATAAATCTGATTTTTGAACAACCAAACGTAGTCTGATTCCTGCTGTCATCATACCTGACCACTAATAATGCCAGTCATTCCATTAAAGAAAATCTAGAGAACTGATTCCCAAAGTTACATGTTGAAATCAAAACGAAGGAGCTGGATATACAATGAGGCAGAGACTATCACCAAATATTGGCAATGCTTTTATAATATGGGATAATATCTCTCAACTTGCACAAATTTTCTTCAATTTGAAATGAAAAATCGTTCATTCTTACAAGTTGCTTCCAGAAGCAGAGACCTCTGTACTGCAAACAGGGCAAACCTTGTTTATTTTCAACCAGTTGTTAATGCACTCAGGATGGTACAGATGTTTGCAGGAAAGAACTGTCAAGGACTCATCATCCTCATAATCCACCCGGCAAATGACACACGAATCATTGCTCCCATGCTGATCACTCCCCGTCTTATAGTTGATTGAAGGTAAACAGGCAATAGTATCAGTCGAAAGGCCTCTGCTCTCAGTTCCTACTACCTCACTCAATGCCAGGAGTTCCTCATATGAAAGTTCATCTGGATCAACATCCTCCCAAGCATCCTGAGAATTAGCACCATGCTCCTCCATGTCCTCAGCATCCTGATCATGTATCCCAGCAAGTGCCAACAGTCTAGCTGCCATATCCCTCTCTTCAGCCCCCTGTAATGCTCTTGCATAGGCTTCATCACTTGAAAACAGCTCGGGATCAAATTCAATGGTGGGGTTATCATGTTCTCCAGCACTAGCATGAGCATGAACATCAAATACATCTTCATCTTCATATCCCTCCTCATTATCGTCATCCTCATCCTCATCACCATCATCATCATCCTCATCTACATCAGTGCCATCATGAAGATCATCAAAATCATCTCCATCATCATGCAAATAACTCTCACCCTCCCAACTTCCATAGTCACTTCCATCATTATTCATTCTCAGCATCATGTATGCCCTTTCCTGCTCTTGAAGGGTTCGAGCGAGGGCGAGGTCAGCATCGACTTGGCTGAGATTAGTGAAAGGCGTTCTGGAAGGTTGTTGAGCGGCGGTTTCAGATCCAGTAGGTTGTTGTTGTTCCCCTTTGTCTTCGTCTTCTTCTTCTTTCTCTTCCATTTTTTCTGGTATGTTTTCCTCGCCGTTCAAATTAGAATGTTCTTCTTCGTGGATATGGTTGTTGGATTTGACTCCTCCGCCGCCGCCGCCTGAACCTAGTTTGGCCGTGTTGACGGTGGCGTTGACGGTGGTGGTGGTGGCGTTGGTGGTGTCGTTCTCCATTGGAATTAGGAAGAAGAAGAAAAAGAGAGTGAATAGAAAACAAGAATGATCGAATTCGAATCNGACGAATCAACCT
TARGET=1298,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1799
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.164639
PRIMER_LEFT_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_SEQUENCE=CCTAATTCCAATGGAGAACGAC
PRIMER_LEFT=1105,22
PRIMER_RIGHT=1392,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.835
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.3038
PRIMER_LEFT_1_PENALTY=0.139129
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=TTCTTCGTGGATATGGTTGTTG
PRIMER_RIGHT_1_SEQUENCE=CCTAATTCCAATGGAGAACGAC
PRIMER_LEFT_1=1263,22
PRIMER_RIGHT_1=1392,22
PRIMER_LEFT_1_TM=59.861
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=0.3199
PRIMER_LEFT_2_PENALTY=0.015253
PRIMER_RIGHT_2_PENALTY=0.304643
PRIMER_LEFT_2_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_2_SEQUENCE=TTCCTAATTCCAATGGAGAACG
PRIMER_LEFT_2=1105,22
PRIMER_RIGHT_2=1394,22
PRIMER_LEFT_2_TM=60.015
PRIMER_RIGHT_2_TM=60.305
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.4191
PRIMER_LEFT_3_PENALTY=0.114470
PRIMER_RIGHT_3_PENALTY=0.304643
PRIMER_LEFT_3_SEQUENCE=GTTGTTGTTCCCCTTTGTCTTC
PRIMER_RIGHT_3_SEQUENCE=TTCCTAATTCCAATGGAGAACG
PRIMER_LEFT_3=1175,22
PRIMER_RIGHT_3=1394,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=60.305
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=0.4297
PRIMER_LEFT_4_PENALTY=0.265053
PRIMER_RIGHT_4_PENALTY=0.164639
PRIMER_LEFT_4_SEQUENCE=ATGGTTGTTGGATTTGACTCCT
PRIMER_RIGHT_4_SEQUENCE=CCTAATTCCAATGGAGAACGAC
PRIMER_LEFT_4=1275,22
PRIMER_RIGHT_4=1392,22
PRIMER_LEFT_4_TM=59.735
PRIMER_RIGHT_4_TM=59.835
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_04460 Ps_contig_mira-and-tgicl-ass_franssen_33279
SEQUENCE=AATTTTTCCTAACTAATTTGATTTTTAAATTTGTGCCCACATCACATTCCCAAAAATAAAGGGTTCTTTAAAAGAAAATGAAGATGATAAATCTGATTTTTGAACAACCAAACGTAGTCTGATTCCTGCTGTCATCATACCTGACCACTAATAATGCCAGTCATTCCATTAAAGAAAATCTAGAGAACTGATTCCCAAAGTTACATGTTGAAATCAAAACGAAGGAGCTGGATATACAATGAGGCAGAGACTATCACCAAATATTGGCAATGCTTTTATAATATGGGATAATATCTCTCAACTTGCACAAATTTTCTTCAATTTGAAATGAAAAATCGTTCATTCTTACAAGTTGCTTCCAGAAGCAGAGACCTCTGTACTGCAAACAGGGCAAACCTTGTTTATTTTCAACCAGTTGTTAATGCACTCAGGATGGTACAGATGTTTGCAGGAAAGAACTGTCAAGGACTCATCATCCTCATAATCCACCCGGCAAATGACACACGAATCATTGCTCCCATGCTGATCACTCCCCGTCTTATAGTTGATTGAAGGTAAACAGGCAATAGTATCAGTCGAAAGGCCTCTGCTCTCAGTTCCTACTACCTCACTCAATGCCAGGAGTTCCTCATATGAAAGTTCATCTGGATCAACATCCTCCCAAGCATCCTGAGAATTAGCACCATGCTCCTCCATGTCCTCAGCATCCTGATCATGTATCCCAGCAAGTGCCAACAGTCTAGCTGCCATATCCCTCTCTTCAGCCCCCTGTAATGCTCTTGCATAGGCTTCATCACTTGAAAACAGCTCGGGATCAAATTCAATGGTGGGGTTATCATGTTCTCCAGCACTAGCATGAGCATGAACATCAAATACATCTTCATCTTCATATCCCTCCTCATTATCGTCATCCTCATCCTCATCACCATCATCATCATCCTCATCTACATCAGTGCCATCATGAAGATCATCAAAATCATCTCCATCATCATGCAAATAACTCTCACCCTCCCAACTTCCATAGTCACTTCCATCATTATTCATTCTCAGCATCATGTATGCCCTTTCCTGCTCTTGAAGGGTTCGAGCGAGGGCGAGGTCAGCATCGACTTGGCTGAGATTAGTGAAAGGCGTTCTGGAAGGTTGTTGAGCGGCGGTTTCAGATCCAGTAGGTTGTTGTTGTTCCCCTTTGTCTTCGTCTTCTTCTTCTTTCTCTTCCATTTTTTCTGGTATGTTTTCCTCGCCGTTCAAATTAGAATGTTCTTCTTCGTGGATATGGTTGTTGGATTTGACTCCTCCGCCGCCGCCGCCTGAACCTAGTTTGGCCGTGTTGACGGTGGCGTTGACGGTGGTGGTGGTGGCGTTGGTGGTGTCGTTCTCCATTGGAATTAGGAAGAAGAAGAAAAAGAGAGTGAATAGAAAACAAGAATGATCGAATTCGAATCNGACGAATCAACCT
TARGET=1348,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1799
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.164639
PRIMER_LEFT_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_SEQUENCE=CCTAATTCCAATGGAGAACGAC
PRIMER_LEFT=1105,22
PRIMER_RIGHT=1392,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.835
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.3038
PRIMER_LEFT_1_PENALTY=0.139129
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=TTCTTCGTGGATATGGTTGTTG
PRIMER_RIGHT_1_SEQUENCE=CCTAATTCCAATGGAGAACGAC
PRIMER_LEFT_1=1263,22
PRIMER_RIGHT_1=1392,22
PRIMER_LEFT_1_TM=59.861
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=0.3199
PRIMER_LEFT_2_PENALTY=0.015253
PRIMER_RIGHT_2_PENALTY=0.304643
PRIMER_LEFT_2_SEQUENCE=TCGACTTGGCTGAGATTAGTGA
PRIMER_RIGHT_2_SEQUENCE=TTCCTAATTCCAATGGAGAACG
PRIMER_LEFT_2=1105,22
PRIMER_RIGHT_2=1394,22
PRIMER_LEFT_2_TM=60.015
PRIMER_RIGHT_2_TM=60.305
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.4191
PRIMER_LEFT_3_PENALTY=0.114470
PRIMER_RIGHT_3_PENALTY=0.304643
PRIMER_LEFT_3_SEQUENCE=GTTGTTGTTCCCCTTTGTCTTC
PRIMER_RIGHT_3_SEQUENCE=TTCCTAATTCCAATGGAGAACG
PRIMER_LEFT_3=1175,22
PRIMER_RIGHT_3=1394,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=60.305
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=0.4297
PRIMER_LEFT_4_PENALTY=0.265053
PRIMER_RIGHT_4_PENALTY=0.164639
PRIMER_LEFT_4_SEQUENCE=ATGGTTGTTGGATTTGACTCCT
PRIMER_RIGHT_4_SEQUENCE=CCTAATTCCAATGGAGAACGAC
PRIMER_LEFT_4=1275,22
PRIMER_RIGHT_4=1392,22
PRIMER_LEFT_4_TM=59.735
PRIMER_RIGHT_4_TM=59.835
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_04461 Ps_contig_mira-and-tgicl-ass_franssen_33279
SEQUENCE=AATTTTTCCTAACTAATTTGATTTTTAAATTTGTGCCCACATCACATTCCCAAAAATAAAGGGTTCTTTAAAAGAAAATGAAGATGATAAATCTGATTTTTGAACAACCAAACGTAGTCTGATTCCTGCTGTCATCATACCTGACCACTAATAATGCCAGTCATTCCATTAAAGAAAATCTAGAGAACTGATTCCCAAAGTTACATGTTGAAATCAAAACGAAGGAGCTGGATATACAATGAGGCAGAGACTATCACCAAATATTGGCAATGCTTTTATAATATGGGATAATATCTCTCAACTTGCACAAATTTTCTTCAATTTGAAATGAAAAATCGTTCATTCTTACAAGTTGCTTCCAGAAGCAGAGACCTCTGTACTGCAAACAGGGCAAACCTTGTTTATTTTCAACCAGTTGTTAATGCACTCAGGATGGTACAGATGTTTGCAGGAAAGAACTGTCAAGGACTCATCATCCTCATAATCCACCCGGCAAATGACACACGAATCATTGCTCCCATGCTGATCACTCCCCGTCTTATAGTTGATTGAAGGTAAACAGGCAATAGTATCAGTCGAAAGGCCTCTGCTCTCAGTTCCTACTACCTCACTCAATGCCAGGAGTTCCTCATATGAAAGTTCATCTGGATCAACATCCTCCCAAGCATCCTGAGAATTAGCACCATGCTCCTCCATGTCCTCAGCATCCTGATCATGTATCCCAGCAAGTGCCAACAGTCTAGCTGCCATATCCCTCTCTTCAGCCCCCTGTAATGCTCTTGCATAGGCTTCATCACTTGAAAACAGCTCGGGATCAAATTCAATGGTGGGGTTATCATGTTCTCCAGCACTAGCATGAGCATGAACATCAAATACATCTTCATCTTCATATCCCTCCTCATTATCGTCATCCTCATCCTCATCACCATCATCATCATCCTCATCTACATCAGTGCCATCATGAAGATCATCAAAATCATCTCCATCATCATGCAAATAACTCTCACCCTCCCAACTTCCATAGTCACTTCCATCATTATTCATTCTCAGCATCATGTATGCCCTTTCCTGCTCTTGAAGGGTTCGAGCGAGGGCGAGGTCAGCATCGACTTGGCTGAGATTAGTGAAAGGCGTTCTGGAAGGTTGTTGAGCGGCGGTTTCAGATCCAGTAGGTTGTTGTTGTTCCCCTTTGTCTTCGTCTTCTTCTTCTTTCTCTTCCATTTTTTCTGGTATGTTTTCCTCGCCGTTCAAATTAGAATGTTCTTCTTCGTGGATATGGTTGTTGGATTTGACTCCTCCGCCGCCGCCGCCTGAACCTAGTTTGGCCGTGTTGACGGTGGCGTTGACGGTGGTGGTGGTGGCGTTGGTGGTGTCGTTCTCCATTGGAATTAGGAAGAAGAAGAAAAAGAGAGTGAATAGAAAACAAGAATGATCGAATTCGAATCNGACGAATCAACCT
TARGET=1393,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1164
PRIMER_LEFT_PENALTY=0.114470
PRIMER_RIGHT_PENALTY=2.001949
PRIMER_LEFT_SEQUENCE=GTTGTTGTTCCCCTTTGTCTTC
PRIMER_RIGHT_SEQUENCE=AGGTTGATTCGTCNGATTCG
PRIMER_LEFT=1175,22
PRIMER_RIGHT=1458,20
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.368
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=3.75
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.50
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=2.1411
PRIMER_LEFT_1_PENALTY=0.139129
PRIMER_RIGHT_1_PENALTY=2.001949
PRIMER_LEFT_1_SEQUENCE=TTCTTCGTGGATATGGTTGTTG
PRIMER_RIGHT_1_SEQUENCE=AGGTTGATTCGTCNGATTCG
PRIMER_LEFT_1=1263,22
PRIMER_RIGHT_1=1458,20
PRIMER_LEFT_1_TM=59.861
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.368
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.75
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.50
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=2.2242
PRIMER_LEFT_2_PENALTY=0.222249
PRIMER_RIGHT_2_PENALTY=2.001949
PRIMER_LEFT_2_SEQUENCE=TAGGTTGTTGTTGTTCCCCTTT
PRIMER_RIGHT_2_SEQUENCE=AGGTTGATTCGTCNGATTCG
PRIMER_LEFT_2=1169,22
PRIMER_RIGHT_2=1458,20
PRIMER_LEFT_2_TM=59.778
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.368
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.75
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.50
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=2.2670
PRIMER_LEFT_3_PENALTY=0.265053
PRIMER_RIGHT_3_PENALTY=2.001949
PRIMER_LEFT_3_SEQUENCE=ATGGTTGTTGGATTTGACTCCT
PRIMER_RIGHT_3_SEQUENCE=AGGTTGATTCGTCNGATTCG
PRIMER_LEFT_3=1275,22
PRIMER_RIGHT_3=1458,20
PRIMER_LEFT_3_TM=59.735
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.368
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.75
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.50
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.6000
PRIMER_PAIR_3_COMPL_ANY=3.75
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=2.4722
PRIMER_LEFT_4_PENALTY=0.470203
PRIMER_RIGHT_4_PENALTY=2.001949
PRIMER_LEFT_4_SEQUENCE=TTTTCTGGTATGTTTTCCTCGC
PRIMER_RIGHT_4_SEQUENCE=AGGTTGATTCGTCNGATTCG
PRIMER_LEFT_4=1222,22
PRIMER_RIGHT_4=1458,20
PRIMER_LEFT_4_TM=60.470
PRIMER_RIGHT_4_TM=59.998
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.368
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.75
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.50
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_04462 Ps_contig_mira-and-tgicl-ass_franssen_33288
SEQUENCE=ACAGACAAAAAGACTAAGGTGTTACACAAACTTTTCTCAACACCACATCAAGGAATTCTCTCAAAACATCTGTTGTGTATGTATACATTCATACATACATACACAAACAAAAGTGACAATATCAAATAGTAACACCTATAATGAATAGAGAAAATGTACAACAGAAAAGAATCAGACACTATTATGTACACATGCACAGATTGAACTACTTAACCGGAAAAAAATTCATCTTCATTCTCCACCTCTCTCGCGTTTGTTATTTTCAATAACTTCCCGTCTCTTCATCCGGTGAAATCGCGGTTAAAAGAGAATATTTTACTTTAAGCGATATTTTGCCCTTCTCCACAAAAAGCCTTGCCCCAGACACAACCCAGTAGCCAGGCAAATCTTGCGGTCCTCTTGTCATTTCTGTTGTGTCAACAAACCTCAGAAGTTTAGGTGATTGAGCAGGTACGGGAGGACCTCCGGGGTATAAGGCAGAGTTGACATTTACGTCCGATGGCTGTGGCGGTGGCAGTTTTTGTGGACCTGAAAACCGAGTGCTGATGAAAGTTGAGATCATTCCGGATTTCTGAGTCAAGCCAGGCGAACCATCCCATTCAGGATTTCTCACACGTGATGCGTTGGCAACTTTACAGAAGTGAAGCCTTAGGAAGAGAACTTTCTTCAGACCAGCTTCTTCCCACTTCGAAGTGAGCACCGGTCACAACAGAATTGTCATCGTAGGATTCAACAGGAGCCGTGCAAATATGAGAGAAACTCTTCCACTGGACCTTTTCGTAATATTTACGCTCTGAAGAGGTGTCGGATACATTCCCATTAGTCTCTCCCTCAAGTTGGAATGTCTTGGGGAGGGAGGAGAGGTGCTGCAAATGGATTGACCAAAACTAATTTTACCTTTTTCCTTTCCCTTACCCAAAAATTCG
TARGET=90,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2151
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.206859
PRIMER_LEFT_SEQUENCE=TTTTCTCAACACCACATCAAGG
PRIMER_RIGHT_SEQUENCE=AAAATAACAAACGCGAGAGAGG
PRIMER_LEFT=31,22
PRIMER_RIGHT=262,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.793
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.6037
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.595491
PRIMER_LEFT_1_SEQUENCE=TTTTCTCAACACCACATCAAGG
PRIMER_RIGHT_1_SEQUENCE=TCTCTTTTAACCGCGATTTCAC
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=309,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.595
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.6695
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.661199
PRIMER_LEFT_2_SEQUENCE=TTTTCTCAACACCACATCAAGG
PRIMER_RIGHT_2_SEQUENCE=AATAACAAACGCGAGAGAGGTG
PRIMER_LEFT_2=31,22
PRIMER_RIGHT_2=260,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.661
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.6788
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.670525
PRIMER_LEFT_3_SEQUENCE=TTTTCTCAACACCACATCAAGG
PRIMER_RIGHT_3_SEQUENCE=AAATAACAAACGCGAGAGAGGT
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.329
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=231
PRIMER_PAIR_PENALTY_4=0.7357
PRIMER_LEFT_4_PENALTY=0.528861
PRIMER_RIGHT_4_PENALTY=0.206859
PRIMER_LEFT_4_SEQUENCE=TTCTCAACACCACATCAAGGAA
PRIMER_RIGHT_4_SEQUENCE=AAAATAACAAACGCGAGAGAGG
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=262,22
PRIMER_LEFT_4_TM=60.529
PRIMER_RIGHT_4_TM=59.793
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_04463 Ps_contig_mira-and-tgicl-ass_franssen_33293
SEQUENCE=ATAGTAGTATATATAAATTGAAATTGAAATTGAAATYGGGATGGAAAGAGAAAATGGAAGGAATACCTCACCCAGTACCTCGAACTGTTGAAGAAGTTTTCAGCGATTATAAGGGTAGACGCGCTGGTTTGATCAAAGCCCTAACTACTGACGTTGAAAAGTTTTACCAGCTCTGCGATCCCGACAAAGAGAATTTGTGCTTATATGGATATCCAAATGAAACATGGGAAGTGAATTTGCCTGTTGAAGAAGTGCCTCCCGAACTTCCCGAGCCAGCGTTAGGTATAAACTTTGCTAGGGATGGCATGCAAGAGAAGGACTGGTTATCACTGGTTGCAGTTCACAGTGACTCATGGCTGCTTGCTGTTGCTTTCTATTTTGGTGCTCGCTTTGGATTCGGTAAGAATGAAAGGAAAAGGCTTTTTCAGATGATAAATGAGCTGCCAACTATCTTTGAGCTCGCAACAGGATCTGCTAAGATAGCAAAGGACCAACACCAACTGCCTGCTCACAACAATGGTAGCAATAGCAAATTCAAATCAAGTGGAAAGTCCCGCCAGTCTGAGTCTCAGACCAAGGGTGTGAAGATGTCTGCACCGCCCAAAGAAGAGGATGACAGTGGAGAAGAAGATGATGATGATGAAGAAGATGATGAACAAGGTGCAACCTGTGGTGCTTGTGGTGATAATTATGGCACTGATGAGTTCTGGATCTGTTGTGATATGTGCGAGAGATGGTTCCATGGTAAATGTGTTAAAATTACTCCTGCTAAGGCTGAACACATCAAGCAATACAAGTGTCCCGGCTGCAGTACCAAGAAGCCAAGAATTGCGTAGCTCTGAAGCTTTGGGCCAATGTCAAGATTTAGAATGTTTGATAGCATTTTTATAAGTGAAAGTGAAACCATTGTTGCCTGTTCTAGTCAATGCACTTTAGTGACTACGCAGATGTAATTTCAAGTTTTGTGATCGTTCATTCCTACTGTCTACTCTCATTGGGAATTTGCATCTTTAAGTAGTCACTGGCAACAAAAATATTATATAAATAGATTATGCAGTTTCTGGTTTTAATTTTAATGAATATTTTGTTTATGAC
TARGET=630,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04464 Ps_contig_mira-and-tgicl-ass_franssen_33298
SEQUENCE=CCTAACAACCACCAATCAACACATTATCACAAACTTTCAATCTTGGCTGCTTTTATTTGATCTTTTGAGCAAGTAAAGGAAACNGGTTTTTCAAAACCGTTACGTTAACGTTGTTCCAAACATCAGATAAGGTGGGGAGTGCAGCGATTTTCTCAGCAACAATCATGTCTTCCGGAAGAACAGAGCCGAAAACAGTGTATTCTTGTTTCCATTCTGAGTGATCGGCTAAGCTAATAAAGAATTCTGGACCCGAACCAATCAACGCAACTGAGCCTCTTCTTAAGAGCGGACAATCTTCTAAAGGAAGTTTATGGAACGGTGTGCCTTGTGCTTCTAATGTCCCTTGAATTAACGCATACGGGGGACCAAAACCGGCATTTTTTATATGGTTTCCTTCCGAATCCCATGATTGACCTCGGCTCTCTGCACGGTATAATTCGCAGCCAGCGCAATGGTGCAAGGGCAACAGTTCTAGAATATAGGCAACTGTGTGCGGAGCACAGTCAGGGAAAAGTTTGATTTGAATAGTTCCGTGTTCAGTTTCGAACCCAATAATACCCTCCCCTTTTCCAAAAACGAGACCAGAAGTCCAAGTAATTTTCTCTCCAAAAGGATCTTCGTCTTGCCGTTGAGGGGCTAAGCTGTCCTTTTGTTTCTTCAACCAACACTCACCGAATTTGGAGCCGCAATTCTCCCGATTTCCACAAAACACCCAAGAATCACATAGACAAGGTCCATCTTTCCCAGTGCACATAGCTTTACAAGCATTGCAACATCCTTCAGAAGTGTTGAATTTGAAATCAGAACCCCATTTCACAGCTGAACCCCAAAGCTCCAAATCTTCAACCCCTCTACAACACCCTCCTCCAGTTTTTTCACCAACTATAGCAAAGGAAGACGTTGACCCAGCTCTAAACTCCGATACAGAACCACTTCTGGTTACCGCAGAGAAGAAAAGATAGGCCAAACCGCAAGAAGCCAAAAACACAAAAAGAAGAACCAAACGGTTTGCTAGAACGGTGTTCGATTCAATGTTCTTACGAGGAGCCATTCAGGAGTTGGATAAGGCGTTGACCCAAGTTAGTTAGTTGACTAATTGTTGAATTTGGGACAGAAATAGAAAAGTGGAAATTCAATATACAGAACTAGATGAAGAAAGAGGAATGGATGGTTCAGAGAGACATAGTCGTACAATTTTGTCGTTTGAATGTGTTCCTGCTCCAACAACTTTGATGCGTTTATGTTGTTTGATTGTTCGGAAATTGGAATTGTNATTATTTCTGCTACTAAACTTTTTTTTA
TARGET=1079,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0621
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.058463
PRIMER_LEFT_SEQUENCE=TAGAACGGTGTTCGATTCAATG
PRIMER_RIGHT_SEQUENCE=GTCTCTCTGAACCATCCATTCC
PRIMER_LEFT=1012,22
PRIMER_RIGHT=1181,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.942
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.0635
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=TAGAACGGTGTTCGATTCAATG
PRIMER_RIGHT_1_SEQUENCE=TGAACCATCCATTCCTCTTTCT
PRIMER_LEFT_1=1012,22
PRIMER_RIGHT_1=1174,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.0635
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.059875
PRIMER_LEFT_2_SEQUENCE=TAGAACGGTGTTCGATTCAATG
PRIMER_RIGHT_2_SEQUENCE=CTGAACCATCCATTCCTCTTTC
PRIMER_LEFT_2=1012,22
PRIMER_RIGHT_2=1175,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.940
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.0635
PRIMER_LEFT_3_PENALTY=0.003589
PRIMER_RIGHT_3_PENALTY=0.059875
PRIMER_LEFT_3_SEQUENCE=TAGAACGGTGTTCGATTCAATG
PRIMER_RIGHT_3_SEQUENCE=ACCATCCATTCCTCTTTCTTCA
PRIMER_LEFT_3=1012,22
PRIMER_RIGHT_3=1171,22
PRIMER_LEFT_3_TM=59.996
PRIMER_RIGHT_3_TM=59.940
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=0.0635
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.059875
PRIMER_LEFT_4_SEQUENCE=TAGAACGGTGTTCGATTCAATG
PRIMER_RIGHT_4_SEQUENCE=TCTGAACCATCCATTCCTCTTT
PRIMER_LEFT_4=1012,22
PRIMER_RIGHT_4=1176,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=59.940
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_04465 Ps_contig_mira-and-tgicl-ass_franssen_33312
SEQUENCE=ATCTCGTCAATCGGGGGGCTTTTTCTCGACCTAGACCGATAGTTTATGCTAAATCACGCTCCGGATGCGCACTGCGCACCCACTCTATCGAATTTTTGCGATAAGCAGTTAAATTAACAGCTTCAACTCGTTCATTTTCTCGAGAAAATTTCGTTCAACTGAGAAATTCATTTTCCCGGGAAACAAACAAACACACTCTCTCATCTCCGATGCGAATCCTTTCCTCCCACCGTCGATGACCGTCGTCGATATATGCCGCCGTTCAAGTTCCCTTTCGAATCAAACAATGGATCTATGCTTCCGTAGTTTTCCCTGCCTCTCTGATCCTGCTCAAAGGTCTTCCTCGTGCTTGAAGCTGGTCTTGCTCTTGACCCATCTGATTTATGCTGGTGTTCTTTTCTTATTTGATGGAGATTTGGTGGAGAAGACTAAGAAAGAACCGTGGTTCACTGCTTTATACTTGCTGCTGTTTGTTGTTACTTTAGGACAATACTATGCTATATCTATTTCCAACCCAGGTTATGTCTTGGATGTCATGAAGAACTTAAATAAGAGAAATGTAGTACACACTAAGACATCGGAAGCATCAAATCAACCAGCTTCAAGCAAAAATGGGAGCATTGTAATTTCCATCGAGGGGAGTCAGTCTGAAAAAGGTGTTGCTGGAAGCAATGCAACATATTGGTCAAAGTTGGTGTCCGGATTGTATCCTCCTGGATCAACAATCAGAACATGGACGTGCACCTACTGCAATGTGGAGCAGCCTCCACGGGCAAAGCATTGTCATGATTGTGACAAATGTGTTCTTCAGTTTGATCATCACTGTGTTTGGCTTGGAAATTGCATTGGCCAGGGTAATCATTGCCAATTTTGGTGGTACCTTTGTGAAGAGACGGCAGTGTGCATCTGGACTGGTTTCTTGTATATTTCACACCTGACGGCTCACATTACAATGGTTTGGTGGCAGGATGCCATCATGATACTACTCTTGATCGCGCTGTCAATTTCACTTATCTTTCTGCTTCTCCTACTGCTATTTCATAGTTATCTAATTCTAACCAATCAGACTACCTATGAACTTGTAAGGAGAAGACGGATTTCTTATCTAAGGGGAATTCCAGAAAGAGTGCACCCTTTTAGTGAAGGAGTTTGCAGAAATATATACAGCTTCTGCTGTTCATACCGTTTTGAACCAATACCTACACCGCAAGAGATAGAGGAAAAGTCAAGGCAATACACATGCTCTGATGTTGTAACTTGCAGGTTGTTGCTGATTTGGTTTTAAGTTTTGTACAAAGGTTAAGTAAACGTTCCCTTTCGACCAATATTTATTA
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5135
PRIMER_LEFT_PENALTY=0.493727
PRIMER_RIGHT_PENALTY=0.019805
PRIMER_LEFT_SEQUENCE=GCACCCACTCTATCGAATTTTT
PRIMER_RIGHT_SEQUENCE=GTTTGATTCGAAAGGGAACTTG
PRIMER_LEFT=75,22
PRIMER_RIGHT=284,22
PRIMER_LEFT_TM=59.506
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.5135
PRIMER_LEFT_1_PENALTY=0.493727
PRIMER_RIGHT_1_PENALTY=0.019805
PRIMER_LEFT_1_SEQUENCE=ACCCACTCTATCGAATTTTTGC
PRIMER_RIGHT_1_SEQUENCE=GTTTGATTCGAAAGGGAACTTG
PRIMER_LEFT_1=77,22
PRIMER_RIGHT_1=284,22
PRIMER_LEFT_1_TM=59.506
PRIMER_RIGHT_1_TM=59.980
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=0.8636
PRIMER_LEFT_2_PENALTY=0.493727
PRIMER_RIGHT_2_PENALTY=0.369874
PRIMER_LEFT_2_SEQUENCE=GCACCCACTCTATCGAATTTTT
PRIMER_RIGHT_2_SEQUENCE=GGGAAAACTACGGAAGCATAGA
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=312,22
PRIMER_LEFT_2_TM=59.506
PRIMER_RIGHT_2_TM=59.630
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.8636
PRIMER_LEFT_3_PENALTY=0.493727
PRIMER_RIGHT_3_PENALTY=0.369874
PRIMER_LEFT_3_SEQUENCE=ACCCACTCTATCGAATTTTTGC
PRIMER_RIGHT_3_SEQUENCE=GGGAAAACTACGGAAGCATAGA
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=312,22
PRIMER_LEFT_3_TM=59.506
PRIMER_RIGHT_3_TM=59.630
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.8760
PRIMER_LEFT_4_PENALTY=0.493727
PRIMER_RIGHT_4_PENALTY=0.382228
PRIMER_LEFT_4_SEQUENCE=GCACCCACTCTATCGAATTTTT
PRIMER_RIGHT_4_SEQUENCE=GAAGACCTTTGAGCAGGATCAG
PRIMER_LEFT_4=75,22
PRIMER_RIGHT_4=341,22
PRIMER_LEFT_4_TM=59.506
PRIMER_RIGHT_4_TM=60.382
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_04466 Ps_contig_mira-and-tgicl-ass_franssen_33318
SEQUENCE=ATACAGTTTCGTATTTCTTAACATACTGATTGACATCACACGATGGCATAACAGAATTTTCCATTAATATTCATTCACAAACAACCAATAACTAAATAACCTTTCACAGCTAATAGAAACAAATTAACAGGAATTCATTCCTAACGGAAAATTAGTTCTGACTTCACATAACAAGTCACTAACAAATAATTAAAATCCAACCATTAACTCCAAAATCCTACCAAACTACATTAATAGTTCCACCAGAATTTGAAAGCTAACAAACCTACGGCATACCGTGCATCCATCCATCGACAATTGGCGTAGCAGGGTTTAGAACTTTCCTATACCAACAGTTCCTTCAAGTAACCTCTCAGAAGTTGTGCAAGTTCGGTTCCGTCCGTGACTTGTCCATTGCAACAAACCCGGGACACACCTCCGTCTCGAAGGTTGGTTGATTGTTCTCTTCTTGCGTCATTGCTTTGCTTAGCTTAGAGTTTGTGAGTAATCGGATACGAATAGGATTTTTTCGGTTAGAATTGAGGCAATTCAAGATTGAGCTGGCGGAGAAGGGAGAGGATTCGGTACCGGTGTGTGTCGAAATCCCATTATGATTGCGAGATTGACGCGATTTAGAGACATCCCTTCTTCATCTGGTAATCGTGTTGGGTTCATGCTTGAAGAAGAAAACGTGAAGCCATGGAGATGAGCGTCGAGGTCAATTCGAAGGTTGAAGATCGCAGAGCATTTTCTCTTCATGGACTTCGTTTGATTCTGTTGCAGCGGAAGGAGAAAACGTGCATTGAAACCGCGGCCGGCGAATGTCGCGGATGACCATGTTTGGACGCGTTTCNAGAAGTTGTTCTTGGCCA
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6603
PRIMER_LEFT_PENALTY=0.363436
PRIMER_RIGHT_PENALTY=0.296846
PRIMER_LEFT_SEQUENCE=AGTTCCACCAGAATTTGAAAGC
PRIMER_RIGHT_SEQUENCE=TAAGCTAAGCAAAGCAATGACG
PRIMER_LEFT=235,22
PRIMER_RIGHT=472,22
PRIMER_LEFT_TM=59.637
PRIMER_RIGHT_TM=59.703
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.7089
PRIMER_LEFT_1_PENALTY=0.412035
PRIMER_RIGHT_1_PENALTY=0.296846
PRIMER_LEFT_1_SEQUENCE=CCAACCATTAACTCCAAAATCC
PRIMER_RIGHT_1_SEQUENCE=TAAGCTAAGCAAAGCAATGACG
PRIMER_LEFT_1=196,22
PRIMER_RIGHT_1=472,22
PRIMER_LEFT_1_TM=59.588
PRIMER_RIGHT_1_TM=59.703
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=277
PRIMER_PAIR_PENALTY_2=0.7664
PRIMER_LEFT_2_PENALTY=0.363436
PRIMER_RIGHT_2_PENALTY=0.402961
PRIMER_LEFT_2_SEQUENCE=AGTTCCACCAGAATTTGAAAGC
PRIMER_RIGHT_2_SEQUENCE=ACAATCAACCAACCTTCGAGAC
PRIMER_LEFT_2=235,22
PRIMER_RIGHT_2=440,22
PRIMER_LEFT_2_TM=59.637
PRIMER_RIGHT_2_TM=60.403
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.7832
PRIMER_LEFT_3_PENALTY=0.363436
PRIMER_RIGHT_3_PENALTY=0.419726
PRIMER_LEFT_3_SEQUENCE=AGTTCCACCAGAATTTGAAAGC
PRIMER_RIGHT_3_SEQUENCE=CAATGACGCAAGAAGAGAACAA
PRIMER_LEFT_3=235,22
PRIMER_RIGHT_3=458,22
PRIMER_LEFT_3_TM=59.637
PRIMER_RIGHT_3_TM=60.420
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.8150
PRIMER_LEFT_4_PENALTY=0.412035
PRIMER_RIGHT_4_PENALTY=0.402961
PRIMER_LEFT_4_SEQUENCE=CCAACCATTAACTCCAAAATCC
PRIMER_RIGHT_4_SEQUENCE=ACAATCAACCAACCTTCGAGAC
PRIMER_LEFT_4=196,22
PRIMER_RIGHT_4=440,22
PRIMER_LEFT_4_TM=59.588
PRIMER_RIGHT_4_TM=60.403
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_04467 Ps_contig_mira-and-tgicl-ass_franssen_33322
SEQUENCE=CCTTTTTAGTTAGGTAGCACATCACTTCGTTTTATATATAAAAAACAAGAGAGCAGGCATATGTTATTATAACTGTAAACATCATCTAATTTAACTCCGATTGGATTTTACTTAAATAACTGTTCCAGTACTAAATTCCGACAAAACTCATTATTTAGCAATACAGATGAAGAAAACAGAAAAGAGATTGAAAAGCAAAAAAAAGACTGATTGGCACTAAGATTTCCTAACATCTGATTCTGAGATACAAGGGAGTTGTGGAGCCTGCAGGTAACCCTGTTGCTGTATTCTCCCAGAGAAACATCTCATTTTCCTTCAAAGCTCATCTTTAGGTTGTTCTACTTCTTGCTGAGCAGGAGCTTTTGGCTGTTCAACTTCTTGCTGAGCAGCACCAGTTGTGTCCTTGTTCTTTTGAATGAATTCAATGATGTCTTCCTTTGTCCTACCACCGTCGTATTGTGATACCTTTCCACTTGCTGACCTGAAGTACAAAGTGGGATAACCTTGAACCTCAAAGGTATCGCTTGGGATATCGTTGGCAGTTGCATCCAGTTTTGCAATAACAACATCAGGATCACTTTGAAATGAGACAGCAACTTCGTCCAAGATTGGAGCCAATGATTTGCAGTGACCACACCAAGGGGCATAAAACTCAAGCAAAACATTCTTCCCAGAGTTGAAAACTATTTCTTGAAGAGTTTCCCCAACTACCACTTTAACAGGTTCGTTGTTAGTCGCAGGGATAGGTTCAGACTTGACAAATGGAGCAACATCTCCATCCTTGTATGCCTTCAACCAAGCTGCAAGGTGATCTGGTTCCAAATTGGGCTTGAAAAATTTCTTGCCATCAGTATGCTGAATAATAATTAGAGGTACTTGGTCTTCCTTAAGTCCAAAATACTGGAAGGCACCTTGGCTGGACTCAACATCTCCGATAAGAAAGCTAATTCCCTGTTGTTTGTATTGCTCGGCAATTTCACGGTATTTTGCCTTAAAAGCTTCAGTACTTGCAGAAGTGAAGTTGACGAACAACATTGCCTTTGCATTGGGAGAGTTAAAGTATTTGACAACAAAAGGGTGATTGCTTGGTTCATTGTTGAAGATAGTCACAAGGGGAATACTGGATTCTTCAACAAATTTCACTAAAGCTTCAACATTGAAATCCTTGGAGTCAACAAAGAGCTCGTCAAATGGCTTAAATAACCTAACTACAGGGCCGGACACAGATGAATCTCCCCTTGGGAGGTATTTGGCATCCAAGGTGTGAGCAATGTCATAGTCAGCACGCAACTTATCTGCTAATGCAAGGAAGTTATCATACTCCTCCCCAGAGAATTTTGGGAAAACTCCAACAACAACAATTTTTTTATCACCAATAAAAGCACTGGCTTCATCAGCAGATTTAATTTCTGTTGAAGCAGGTCCATTTTGTTTCTTCAAGTATTCGACAATACCATCAGCTTCGCGGGGACCTTTGTATTCTTGAATGTTTTTTCCACCATTCCTCAAAATCTTAATTGTTGGGAATCCCTTAACTTCATATTCAGACGCAAGTTCCTTGTTCTTCTCCTCATTGGCATCAACTTTAGCCAAAACAATTGGAGTTTCAAGACTGCTCAAGATAGAAGCAGCTTTTTCATACTCTGGGGCAAGCTGCTTACAGTGACCACACCAAGGGGCATAAAACTCGACGACGATGAATTCGTGCTTGGTGACAGTGTCATGGAAATTGGTGTTATCCAATGTGAGGACGGATTCCTTGGCCTCTGATTCCTCAGCGGAGATCTGAGAAGGAAGCAACACAAGAAGAGAGAACAAAAAACCGAAAATCGAAACGATTTTAGCCATTGTAGAAAATATGGTATAAAATTTTATGGATTTTCCCCCCGNACTCTGCGTTGATTACCTACTTGTCTTT
TARGET=1350,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0666
PRIMER_LEFT_PENALTY=0.062191
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=GCACGCAACTTATCTGCTAATG
PRIMER_RIGHT_SEQUENCE=ATGGACCTGCTTCAACAGAAAT
PRIMER_LEFT=1282,22
PRIMER_RIGHT=1426,22
PRIMER_LEFT_TM=59.938
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=0.0899
PRIMER_LEFT_1_PENALTY=0.062191
PRIMER_RIGHT_1_PENALTY=0.027715
PRIMER_LEFT_1_SEQUENCE=CACGCAACTTATCTGCTAATGC
PRIMER_RIGHT_1_SEQUENCE=AGCCAGTGCTTTTATTGGTGAT
PRIMER_LEFT_1=1283,22
PRIMER_RIGHT_1=1389,22
PRIMER_LEFT_1_TM=59.938
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=107
PRIMER_PAIR_PENALTY_2=0.0899
PRIMER_LEFT_2_PENALTY=0.062191
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=GCACGCAACTTATCTGCTAATG
PRIMER_RIGHT_2_SEQUENCE=AGCCAGTGCTTTTATTGGTGAT
PRIMER_LEFT_2=1282,22
PRIMER_RIGHT_2=1389,22
PRIMER_LEFT_2_TM=59.938
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.1339
PRIMER_LEFT_3_PENALTY=0.062191
PRIMER_RIGHT_3_PENALTY=0.071684
PRIMER_LEFT_3_SEQUENCE=CACGCAACTTATCTGCTAATGC
PRIMER_RIGHT_3_SEQUENCE=AGAAGAACAAGGAACTTGCGTC
PRIMER_LEFT_3=1283,22
PRIMER_RIGHT_3=1567,22
PRIMER_LEFT_3_TM=59.938
PRIMER_RIGHT_3_TM=59.928
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.1339
PRIMER_LEFT_4_PENALTY=0.062191
PRIMER_RIGHT_4_PENALTY=0.071684
PRIMER_LEFT_4_SEQUENCE=GCACGCAACTTATCTGCTAATG
PRIMER_RIGHT_4_SEQUENCE=AGAAGAACAAGGAACTTGCGTC
PRIMER_LEFT_4=1282,22
PRIMER_RIGHT_4=1567,22
PRIMER_LEFT_4_TM=59.938
PRIMER_RIGHT_4_TM=59.928
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_04468 Ps_contig_mira-and-tgicl-ass_franssen_33324
SEQUENCE=TTTTCTAAGGAACGAGATAAATTAATAAACCAGAGACTTTGTTCATCAATTTAAAACAATTGGGAGTATCTTACAAGTAGTGATTCATTCAACGTAGACGAATGAGTTGCATTCTTTTATTACAATGGAAACAACCATGGACGAACAACAACACAAATACAAGATGAAAAATAATTAGTTCAAAGGAGAAAACATGCATAGGGATCAAAAGAAATCAATATCTAAATAGTTACCAGAAGGCTTCACACCAGGCGCCTTCTTCTCGGCTATGAGTGCTTCTTTCATGGCCTGTCTCGGCACTTCGGGAGGAGTTGCACAACGGATCAGGGCCCAGTTAATATTTTGAAAGAATGGATGTTGTTTTATCTCCGTAGCTCCACGTCTATACGCAAGACGATGCTGAGGCTCTTTCACAAGTAGACCCCTAATCAAGTCTCTAGCAGCAAAGCTAACACTAGGGGATTCGGGAAATCTTAAGGGCTGCCCAACTACATTAAATAAAGTCGCTCGATTCGCCGAACCTTTGAATGGTGTTCTACCAAACAAAAGCTCGTATAAGAATATACCAAATGTCCACCAGTCTACAGCACTGCCGTGACCTTCGCCTTTGATGATTTCAGGCGCCAAATACTCATGCGTGCCAACGAAGGACATTGATCGGGCATTTGTGGGCTCGGCCATAAGCTCAGGGAGAGGTGTCACCTGATTGTGCACGTCATTCTTAGGTTTCACCTTCTTTTCTTTTCTTTTTGGTATTTGCCTGATAGGAAACGCGGCGTGAAGCATGAAGGTTTGATGCAGTCTGGCTGAATTACACAAGTTGGTTCAATGCATGCTGGCTGAATGCAGTATCCCGAGCTTTTAGTCTCCAAGCTGGTGTTTGATGACTTTACGAGAGTTGGACTGACGGCACATCTTAGAGAGAGATCGAAATCTGATAGCATGATGTGACCATCTTCTCTCACCAACACATTCTCAGGTTTGAGGTCTCTGTAGATGATCCCAAGCATGTGCAAGTACTCTAAAGCAAGGAGAACTTCTGCCACATAAAACCTTGCAGCATGCTCTGAAAAATACTTCCCAGGTTGTCGTTGCCTGAGTGCATGGAGATCCCCACCAGGACAAAACTCCATAACCAAGCAAGAGAATGATTCTGTCTCAAAGTGCGTGTACAATGACGGTAGAAATGGATGATCTAGAGACTGCAATATCTCCCTCTCTGTCTGCGCTCTCGGAAGTTTCTTGCGACTTGACAATTCTGTCTTGTTCATTACTTTCATAGCAAAACAAGTCCTTGTGCTGCTTAACTCTGCTAGATATACACTTCCTATGTCTCCACATCCCAATTTCTTCAACAACCGGAAATGTCTCATTTCCAATCCACCGTCCCGAACCCTGATCGCTTGAATCGCTTCCCATCTTACATCATTTGCTTTATGTGGTTTATACAAAGAACTGCTCAAACTAAACTAAGAAAATAAGAAACTGCTCTCATCACTAACATCA
TARGET=734,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3542
PRIMER_LEFT_PENALTY=0.267873
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=ATAAGCTCAGGGAGAGGTGTCA
PRIMER_RIGHT_SEQUENCE=TCAAACACCAGCTTGGAGACTA
PRIMER_LEFT=679,22
PRIMER_RIGHT=883,22
PRIMER_LEFT_TM=60.268
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.3542
PRIMER_LEFT_1_PENALTY=0.267873
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=CATAAGCTCAGGGAGAGGTGTC
PRIMER_RIGHT_1_SEQUENCE=TCAAACACCAGCTTGGAGACTA
PRIMER_LEFT_1=678,22
PRIMER_RIGHT_1=883,22
PRIMER_LEFT_1_TM=60.268
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=0.4097
PRIMER_LEFT_2_PENALTY=0.323355
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=TGTGCACGTCATTCTTAGGTTT
PRIMER_RIGHT_2_SEQUENCE=TCAAACACCAGCTTGGAGACTA
PRIMER_LEFT_2=707,22
PRIMER_RIGHT_2=883,22
PRIMER_LEFT_2_TM=59.677
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=0.4097
PRIMER_LEFT_3_PENALTY=0.323355
PRIMER_RIGHT_3_PENALTY=0.086339
PRIMER_LEFT_3_SEQUENCE=TTGTGCACGTCATTCTTAGGTT
PRIMER_RIGHT_3_SEQUENCE=TCAAACACCAGCTTGGAGACTA
PRIMER_LEFT_3=706,22
PRIMER_RIGHT_3=883,22
PRIMER_LEFT_3_TM=59.677
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=178
PRIMER_PAIR_PENALTY_4=0.4424
PRIMER_LEFT_4_PENALTY=0.267873
PRIMER_RIGHT_4_PENALTY=0.174518
PRIMER_LEFT_4_SEQUENCE=ATAAGCTCAGGGAGAGGTGTCA
PRIMER_RIGHT_4_SEQUENCE=ATCAAACACCAGCTTGGAGACT
PRIMER_LEFT_4=679,22
PRIMER_RIGHT_4=884,22
PRIMER_LEFT_4_TM=60.268
PRIMER_RIGHT_4_TM=60.175
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=206
=
PRIMER_SEQUENCE_ID=SSR_04469 Ps_contig_mira-and-tgicl-ass_franssen_33325
SEQUENCE=ACATGATTTCAGTCCATTGTTTGTTTCAGCATCACCCTCATCTTCAAAAGCGTAAAGCCAGTGCAAACATATTCCCATGGAAGCGGGAACCGCGCTCCGTCTCCATTTCACTCTCCACACTCCCATTTCCGTATCGAATCGAACTTCAAACCCTAAAACCGCATTCACTCTTTCATCTTCTCCTTCTCGTTCTCATTCTTCTACCGTCCACAGTTTCCTCGGCCCAAAGCCCAAACGAGATTTCCTCGCTGATTGGGCCTCACAAAACGACGACGTCGTTCGCCCCCTCCCAATCTACGTTGGAACCGCCTCTCTCTTTGCTGTCCTCTTCAATCGCGCCATCTCCGGCATAGCTCCCGTCGCCGACGCCGGCAGTTCACAATCCAGAGCAGACCTCTTAACTTTAGGATTGGCTGTCACTAATATTTTGGCTGGCCTTGTATGGCTTTCCATAAGACCCAAATCTATTGCTGTGGTAAATCCTCAGGGAGCTGAATGCAAGAAGCTGTGCACTGCCCTCCCTGATGTTGCACTTACTGAGCTGCTCTGGGTGTGGGAATCTCTGTCAGATGTCACCTGCTGCCGATCACTTGTTATTGTTTATGAGAGCAGTTGTGTGCTTCAATTTGGTTTTGCTGCAGAGTCTTCTCCTGGGAATGGTGAGGTTGTAAGTGTGGACACCGATAAATTGATGCAAGGATCAGTGTACCAGGGTGTTGTGAAATCTGGAACTCAGAGTTACTTGGCTAATCTATCTCTATATCCTGGGAAGTCTGAGCTACCATTTCTGCCTTCAAATACTCAGGCTGTAATTTTACAACCACTTGGAGATAAAGGAATTGCAATTATTGGCGGTGACACAATACGCGGTTTCACTACTTCTGATCAGGCATGGATAACGTATATCGGAGAGAAGCTGGATTCTACTCTTGCAAAATATGTGAAACCGCACCCTTACTTAGTTACTTAAGTCTAAAAGAATTTCAAGTAGCCTCTGATGTTTGTTTATAATTGATTTCAGTAGTAATATATCCTTGTTCTTCATTAAGAATACGAGGCTGAAATTGATTCAATGTATTTATTTTAATTAATTATTTACAGCAGTTTTTAAATTGGTCCCTGCCCAGTCTAATTCATTGTTAGTAACATAAAGATATCGTAGAAGATTAAATCAGCAATTCCCCCCTAGAATTGTAGAGTTCGGACATTCACACTCTGAAATTTGGGAATAAATAAATAAAAATT
TARGET=1228,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04470 Ps_contig_mira-and-tgicl-ass_franssen_33334
SEQUENCE=AGTACTAACTTTCTANCCTTTCCCTCTCTCTCTCCAATCCTTTTTTTCTTCACTAACCCATTTTTTCACANACCCTTTTGCATAAATCCAAAAACTCACACACCCTTTTAGCCCATACTTCACCTTTCTCTCTCTTTGAATCCACTTTGTGACTGTGATGATCATAAAGTTATCATCTTTTTGAGTTATGGGTTCTTGTTCTTCAATTCAGAGAAAACCCAACACAGATAACAATAACATGAAGGTGAAAGTTTCATTTTTTGGATCCAAAACTGAGAAACTTGTCGTTCCTTCATCACCCATTAAGGAACAAACAAAAAATGGTGACTTTATGTTGTCGCCTTCTAAGTCAACGACCAATTTCATTAACTATGGTAGTAAAGAGGAAGCATTTTTTGATTCCAAACCTTGGATGGATTCAGATTGTGAAGATGATTTCTATAGTGTCAATGGTGACTTTACACCGTCTCGTGGTAATACTCCAATTCACCATGCTTTTGCGACACTTGATGTGAACAAAACCCCGTCTCAGAATAGAGTTTCACCTTCTCCATCCGAATCATCGCCCGAAAAGAAAAAGAAACTTTTGGAGCTTTTCAAAGATAGTGTGAAAGATAACCAAGGTGATGATAGTAAAGAAAAGAGACAAGTGAAATCAACTATACAAGATGTTCTTCCTAAGTCTTCACAATCAACTCCTTATTGCTCAAGAGCTAACTCTGCTAGCAGTAGTGAAAGAATCACGAGTGGTGATCGTGTATCGGGTAAAGAGAAATCGCATAAGTCTTCGTTGTTTTGTATTCCGAGTTTGACTTCGTGTCGTAGCTCTAGGGAGAGGAGGAGGAAGACAAGTCCTGCAATTGCGGTTGAGGGCAAACAATGAGTTATATTGCAATGTGTTTGAACTGAATTACCATGTTTGTTTGGTTTTGGTTTTTTTGAGGTAAAGTGGTTGAACTCAATTTGATAATGTACATCAATATCCATACTTAAACATAAGGTTTATGAATTTGAAAGCATGTAAACATAAACGGAAGAGATATTTTATATCAATATGTTATATGAAATTATTTGTTACGGTTAGAAGT
TARGET=24,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04471 Ps_contig_mira-and-tgicl-ass_franssen_33339
SEQUENCE=AAGCAGTGGGTATCRRCGAGAGAGAGAGAGAAAGAAAGCAATTGAAGAAAAGTATCGAAGATGAGAGGAAGAAGCTACACTCCATCGCCTCCACCACGTTACAGCAGAAGAGGAGGTGGAGGAGGAGGGAGGAGGACGCAGTCCAAGTCCCCGTCGCCGTTACGCCCCCGTGAAAGTGATCTACCTACCAGTCTTCTTGTTCGTAACCTTCGCCATGACTGTAGGCCTGAAGATCTTCGTAGACCCTTTGGTCAATTGGTCCTCTCAAAGACATTTATCTA
TARGET=18,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04472 Ps_contig_mira-and-tgicl-ass_franssen_33340
SEQUENCE=AAACCCNAATTCACACACACAGCAAACAAGCAACAATTTCCAACAATGGCTTCACACACTTGTTCCAGCAACTCATCTTTCCAATCCTCATTCACCAATAAACTCAAATCCATCCAATTCAACAACAACCGGCATTTCCCCCCAAATTTTCCTTACCCTAGATCTTTAACACCGCCAAGCGCATCCCTTAGAGTCGACGTGGGAGCACCCAAGGCAAGGGTGGAGACGGGGAAGAAGACGAAAGAGGTGATTGAGGATGAAGCGAAGTTCTTGGTGGGTACTTATGCTAGGGTGCCGGTTGTGCTTGAGAAAGGGAAAGGGTGTAAATTGTATGATGTTGAAGGGAATGAGTATTTGGATTTGAGTAGTGGGATTGCTGTGAATGCGCTTGGGCATGGGGATGATGATTGGTTGAAAGCTGTTGTTGATCAAGCTGGTATTCTAACTCATACTAGCAATGTTTATCTCACTATTCCTCAGGTAGAACTTGCAAAGCGTTTAGTAGGTTCATCTTTTGCTGATCGTGTATTTTTCTCAAATTCTGGAACTGAAGCAAATGAAGCAGCTATTAAATTTTCAAGAAAATATCAGAAACACACATGTACTGAAGGGAAAGCGCCAGCTACCGAGTTCATAGCTTTTAGCAATTGCTTCCATGGGAGAACCATGGGCGCAGTTGCCTTGACAAGTAAAGTGCAGTACAGAACACCTTTTGAACCTGTTATGCCTGGAGTGAACTTTATAGAGTACGGAAATGCACCAGCTGCTGTAGAGTTAATTCAGCAGGGCAAAATTGCTGCTGTTTTTGTCGAGCCTATCCAAGGAGAAGGGGGAATATACAGTGCTACAAAAGAATTTCTACAGTCTCTGCGAAATGCTTGTGATGAAGCTGGGACACTTCTTGTGTTTGATGAGGTTCAATGTGGTTTAGGTAGAACAGGACATCTTTGGGCTCATGAGGCATATGGAGTCTTCCCGGACATTATGACACTTGCCAAGCCTCTTGCTGGAGGCCTACCTATTGGAGCCGTCCTCTTAACCGAGCGAGTTGCTTCTTCTATAAAATACGGTGAACACGGGAGTACTTTTGCAGGAGGTCCTCTTATTTGCAATGTCGCCCTTGCAGTTTTGAATAAAATATCAAAACCTAATTTCCTCTCCGACGTATCAAAGAAAGGACTATACTTCAAAGAACTACTAAAACAGAAATTGGGAGGAAATCCCCATGTGAAAGAGATTCGTGGCGTCGGGCTAATTATAGGAATTGATTTAGACGTGCCTGCATCACCGCTGGTAGACGCTTGCCGAAATTCTGGCCTTTTAATATTGACAGCTGGAAAAGGTAATGTTGTTAGGCTTGTTCCACCATTGATTATAACAGAACAGGAGATTGAGCAAGCAGCTGATATTCTGTCCCAAACTTTGCATGTTTTGGATGCCAATAATTCAAAGTAGGAGAAAGAGGCTTTATTTTCTTGCTGGAAAGATCATGGATTTTTCTTGCATGAAGATATTGTTTCAAGGTTATGTAATATTGTATATTAAATAAGTGGCAGATGTGATGATTTTGCATCGATGCTTTCATTGTATGTCAGAAGGGTATGTGGCTATTTTGCCATTTGAAGTGTAATAAACTTTRNNNNTTTTNTTTATCATTTCTCTTACTAAAACC
TARGET=12,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04473 Ps_contig_mira-and-tgicl-ass_franssen_33344
SEQUENCE=ATTCATTTTTGTCTTTCTGTTTGTTCTTTCAGAGAGCGCTGTCTCTCTCTGCAGCAAGAAGCCACCCACCACCGGCCACTCTCTCTCTACNAAATTTAGGGTTCCATACTATCTTGTGAGTTAATTTATATTAGGTTATGTCGTCGCAACAAGGTGGTTCAGAGAACAATGCGATGGATTTTGACCTACCCGACGAGATTCTGGCAGTTATTCCGACGGACCCATACCACCAGCTGGATCTCGCTCGGAAGATAACTTCCATGGCTATTGCTTCTAGGGTTTCGTCTCTTGAGTCTGATACCGGTCGCCTTCGTCAGAAGCTTCTAGACAAGGATAGAGTTATCAATGACCTAGAGGACCGTGTTTCTACTCTCACTCGCGCTTCTCAACAGGCTAACTCTGCTCTTAATACCGCCATTGAAGAAAACGTAAAGCTTACGAAGGAGAGGGATGAATTGGCAGCAACAGTGAAGAAGTTAAGTCGTGACTTTGCCAAGTTGGAGACATTCAAAAAACAAACTGATGCGCTCGCTAGCTGATGACCATCCACCGCAAGCTGAAACCATAGATATTAGAACCTGTGACCAAGCAGTTCCAAAAGCGTATCCTGATAAGGATGATGATGGAAATGACTATACGACACATCATTCACACAGTGGCTCTGCAGATGTGGGTAGAACAATTGAGGAAGCTTCCAGGTATTCAGGGCAAAAGTTTTCTTTGACCCCGTATATCACGCCACGTCTTACACCAACTGCAACTCCAAAAGTGATCTCAACGTCTGGTTCTCCTAGAGGGTATTCTGCTGCTGTATCACCCAATCAAACATCTGGTGCCACCTCTCCGTCCAAAACTTCATATGATGGGCGGTCATCTCTCTCTTCATGGTATCCATCAAGTCAGCAGTCATCAGCAGCAAATTCTCCACCTCGAGGACGATCAATTCCAGGTCGAACTCCAAAGATTGATGGAAAGGAGTTTTTCCGTCAGGCCAGGAGTCGCCTGTCATATGAGCAGTTCAGTGCATTTCTTGCTAACATTAAGGAATTAAATGCTCAGAAACAAACACGAGAGGAAACTTTAAGAAAGGCAGATGAGATATTTGGCTCAGATAATAAAGATCTTTACCTATCTTTTCAAGGATTGCTTAACCGAAATGTACGCTAGGACTGAAAATAGCATTGCTTGTAATTATGAGTTGGTCATTAACTTGCTCAGCACTCTTGGGTGCGTTTTTCCGACTGGTTTCTCTTTGATTCATTGAATCAAAATCCTACTAAGCATTTCTATGAGCTATTTAATTCGAGATAGTACATTTGATCTACGATTTTCATTTTCTGAATATTTATACATTCCCTGTTGCATGTTGTATATATAGAATGTCAAGCAAAGCTAAAAATGCTGATTGAATTTTGAAATATTGAGGTTTATTGTACACGTTTGAATGGGTTATGGGTGTAATGTGAGTTTTAGACAAATCTTGGAATCAAAATGTTTGCTCAGTTAAATCAAGTCTCTGATGGTGTTGCATCTGACACAACCCAGTTCTTCAGGAAGTATAGTTTTGTAACAAAGCGGTCATGGTTTATGG
TARGET=79,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7791
PRIMER_LEFT_PENALTY=0.695219
PRIMER_RIGHT_PENALTY=0.083883
PRIMER_LEFT_SEQUENCE=CTGTTTGTTCTTTCAGAGAGCG
PRIMER_RIGHT_SEQUENCE=CAGACTCAAGAGACGAAACCCT
PRIMER_LEFT=16,22
PRIMER_RIGHT=296,22
PRIMER_LEFT_TM=59.305
PRIMER_RIGHT_TM=59.916
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.8805
PRIMER_LEFT_1_PENALTY=0.695219
PRIMER_RIGHT_1_PENALTY=0.185267
PRIMER_LEFT_1_SEQUENCE=CTGTTTGTTCTTTCAGAGAGCG
PRIMER_RIGHT_1_SEQUENCE=TGTTCTCTGAACCACCTTGTTG
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=167,22
PRIMER_LEFT_1_TM=59.305
PRIMER_RIGHT_1_TM=60.185
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.8815
PRIMER_LEFT_2_PENALTY=0.695219
PRIMER_RIGHT_2_PENALTY=0.186309
PRIMER_LEFT_2_SEQUENCE=CTGTTTGTTCTTTCAGAGAGCG
PRIMER_RIGHT_2_SEQUENCE=CTTGTTGCGACGACATAACCTA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=152,22
PRIMER_LEFT_2_TM=59.305
PRIMER_RIGHT_2_TM=60.186
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=0.9376
PRIMER_LEFT_3_PENALTY=0.695219
PRIMER_RIGHT_3_PENALTY=0.242357
PRIMER_LEFT_3_SEQUENCE=CTGTTTGTTCTTTCAGAGAGCG
PRIMER_RIGHT_3_SEQUENCE=GAAACCCTAGAAGCAATAGCCA
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=282,22
PRIMER_LEFT_3_TM=59.305
PRIMER_RIGHT_3_TM=59.758
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=1.0620
PRIMER_LEFT_4_PENALTY=0.695219
PRIMER_RIGHT_4_PENALTY=0.366796
PRIMER_LEFT_4_SEQUENCE=CTGTTTGTTCTTTCAGAGAGCG
PRIMER_RIGHT_4_SEQUENCE=CATTGTTCTCTGAACCACCTTG
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=170,22
PRIMER_LEFT_4_TM=59.305
PRIMER_RIGHT_4_TM=59.633
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_04474 Ps_contig_mira-and-tgicl-ass_franssen_33350
SEQUENCE=CGTAGGCGTTCGATGGAAAAAGTCAATAATTGACCACGCACTTTATGTCCTTCACATCAAGACCCCGTGCAGCAACATCGGTGGCAGTCATTATTGGACTTCTGCCACTCTTAAATTCAGCCAAAACCCAGTCCCTTTCAGACTGGTTTTTATCACCATGGATAGATAATGCTGGCCATCCATCCATTCTCAACTGTTTTGTAACTTGGTCACATCCCTTTTTTGTTTCCGTAAATATTAGAATTCGGCTCCCGTCCATCACTTCTTTCAGCAGCTTGATCAGTCTGTTATATTTCTCCATGTCAGTGACAACTTCAACAACTTGATTTATAGATTGGTTTGCTTTTAAATATGGTGACCCAATAACTACCTTATATGGGTTGTGCAGAAACTGTCTTGCCAGAGCTTCAACCTCCTTTGGCCATGTTGCACTCCAGTATAATGTCTGCCTATCTGGTCGAATCTGGTTTACAATCTTCCGAATCTGAGGTTCAAAACCCATATCCAACATCCTATCAGCTTCATCCAAGACAAGGTATGTCACTCTCTGGAGGTTTGTGTGCCGAGCTTCTAACATATCTATTAGACGACCAGGTGTAGCAATAACAATCTCAACACCTATTCTAAGATCACGAATTTGAGGGCCCTTTGGAGCACCACCGTATATGCATGTACATCTAATATTTGACCGTGATCCAAATTTCATAGCTTCCTGTTGAATTTGAACAGCTAACTCTCTAGTTGGTGCTAAAACCAGGACAACAGGACCCTCCCCTTGTACCAGTCGAGGTTGTGCACTGACATGAACTATAGCCGGAAGTAAATACGACAGAGTTTTACCCGAGCCAGTCTCAGCAATGCCAACTAAATCTCTACCCGTAAGAGCCATGGGCCAACCCTGAGCCTGAATTGGAGTAGGTTCAACAAAACCCAACTTGGCAATAACCTCAAGGCAAATAGATCAGGAAAATTAGCCTCGTGAAAAGACCG
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0400
PRIMER_LEFT_PENALTY=0.002623
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=CTTAAATTCAGCCAAAACCCAG
PRIMER_RIGHT_SEQUENCE=GACAGTTTCTGCACAACCCATA
PRIMER_LEFT=109,22
PRIMER_RIGHT=395,22
PRIMER_LEFT_TM=59.997
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.1922
PRIMER_LEFT_1_PENALTY=0.002623
PRIMER_RIGHT_1_PENALTY=0.189596
PRIMER_LEFT_1_SEQUENCE=CTTAAATTCAGCCAAAACCCAG
PRIMER_RIGHT_1_SEQUENCE=CAAGCTGCTGAAAGAAGTGATG
PRIMER_LEFT_1=109,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=59.997
PRIMER_RIGHT_1_TM=60.190
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.2037
PRIMER_LEFT_2_PENALTY=0.002623
PRIMER_RIGHT_2_PENALTY=0.201035
PRIMER_LEFT_2_SEQUENCE=CTTAAATTCAGCCAAAACCCAG
PRIMER_RIGHT_2_SEQUENCE=ACAGACTGATCAAGCTGCTGAA
PRIMER_LEFT_2=109,22
PRIMER_RIGHT_2=287,22
PRIMER_LEFT_2_TM=59.997
PRIMER_RIGHT_2_TM=60.201
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.2229
PRIMER_LEFT_3_PENALTY=0.198196
PRIMER_RIGHT_3_PENALTY=0.024665
PRIMER_LEFT_3_SEQUENCE=ACCATGGATAGATAATGCTGGC
PRIMER_RIGHT_3_SEQUENCE=TTATACTGGAGTGCAACATGGC
PRIMER_LEFT_3=154,22
PRIMER_RIGHT_3=441,22
PRIMER_LEFT_3_TM=60.198
PRIMER_RIGHT_3_TM=60.025
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.2355
PRIMER_LEFT_4_PENALTY=0.198196
PRIMER_RIGHT_4_PENALTY=0.037339
PRIMER_LEFT_4_SEQUENCE=ACCATGGATAGATAATGCTGGC
PRIMER_RIGHT_4_SEQUENCE=GACAGTTTCTGCACAACCCATA
PRIMER_LEFT_4=154,22
PRIMER_RIGHT_4=395,22
PRIMER_LEFT_4_TM=60.198
PRIMER_RIGHT_4_TM=60.037
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_04475 Ps_contig_mira-and-tgicl-ass_franssen_33352
SEQUENCE=GTATCAACGCAGAGTANACGACTAGTTTCTCTTTTGGAAACTGACCAACAAATAAAACAACACTCTTGTTTGTGTCCACACTCACTCCCTCCCTCCTTCTCCCAATCTCCTCTATACCATTTTCATTTTGTCTCAATAAGGATAAACAACATGGTTCCTCCCATGGAAGCATCTCAATTGAACTAAAGATCTAATATATATCCAACATGTCTCACACTTTCCCCATGGATACCTTGTACTGCTCCGAAGAACATTGGGAGGAACAAGA
TARGET=85,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6797
PRIMER_LEFT_PENALTY=1.656116
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=TCTCTTTTGGAAACTGACCAAC
PRIMER_RIGHT_SEQUENCE=TCTTGTTCCTCCCAATGTTCTT
PRIMER_LEFT=27,22
PRIMER_RIGHT=267,22
PRIMER_LEFT_TM=58.344
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=1.6797
PRIMER_LEFT_1_PENALTY=1.656116
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=TCTCTTTTGGAAACTGACCAAC
PRIMER_RIGHT_1_SEQUENCE=CTTGTTCCTCCCAATGTTCTTC
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=266,22
PRIMER_LEFT_1_TM=58.344
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=1.7110
PRIMER_LEFT_2_PENALTY=1.656116
PRIMER_RIGHT_2_PENALTY=0.054900
PRIMER_LEFT_2_SEQUENCE=TCTCTTTTGGAAACTGACCAAC
PRIMER_RIGHT_2_SEQUENCE=GTACAAGGTATCCATGGGGAAA
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=238,22
PRIMER_LEFT_2_TM=58.344
PRIMER_RIGHT_2_TM=59.945
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=1.7244
PRIMER_LEFT_3_PENALTY=1.656116
PRIMER_RIGHT_3_PENALTY=0.068302
PRIMER_LEFT_3_SEQUENCE=TCTCTTTTGGAAACTGACCAAC
PRIMER_RIGHT_3_SEQUENCE=TACAAGGTATCCATGGGGAAAG
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=237,22
PRIMER_LEFT_3_TM=58.344
PRIMER_RIGHT_3_TM=60.068
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=1.8397
PRIMER_LEFT_4_PENALTY=1.656116
PRIMER_RIGHT_4_PENALTY=0.183585
PRIMER_LEFT_4_SEQUENCE=TCTCTTTTGGAAACTGACCAAC
PRIMER_RIGHT_4_SEQUENCE=GTTCTTCGGAGCAGTACAAGGT
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=251,22
PRIMER_LEFT_4_TM=58.344
PRIMER_RIGHT_4_TM=59.816
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_04476 Ps_contig_mira-and-tgicl-ass_franssen_33353
SEQUENCE=ATACTTACTTTATTATTATGTCTATAAGTTTTGTAGCTCTATCTTATGGGTTTCCTTGTTTTCATGATAAATTGTCTATTCACACAACTCATAAGTCATGCCCTCTACTTGTAAGGTAAGACTTTTCCCTTAGAAGAAAAATCACAATTAGTTAAGATAGGTCTTGAAAAATAGTACCAATATCTTTAACCATAAGTTTCAAGATATAGAAGAAATTCATTCATTCACCTTTGTCCTTCATTTGAAGAAGTTGCATGGTCCATCCTTTTGTGGTTTTGATTTTAGCTTACTCCTAGGTACTTAGATTTGTTGTCGGATATCACCTCCTTAATTACTCCGAAATTTTTCATAGTTGATATTTAATTATTATTTTTGTATATTTGATAGAATGAAGATGTGAAATTTTGATAGAAAAGAGACCATGTCTTTTGTTAGTTTATGTGTTACCTTAAGCCTTGTTTTTGGGTTTCTTATATTGTTCATATTTGCACAAGTTTTCTATTTTTATGGTGGAAAAAGAGAAGAACAAACTTAGATATTGAAATGGAACAAGGTAGTTTTCAAAAGGGGGTGTTTTATTGGTGGTGTTGGAAGAAAACATCACGTTCTTTAGATGTTACAAACGTTAATAGAGACATAGAAACCTCAAATCATGAACAAGACATAGAATTGGGTGTTGAAGGCAAAGATTTGCTTTTGAAACCGTATGGTGAAGAAAGTTTGGAATTGGAATTGATGAGGTTGCATAATCTCCCTGGTCCACCAAGATTTCTCTTCACAATCACAGAAGAAACAAAGGAAGATTTGGAATCTGAAGATGGGAAATCGAAATGCGGAAGAAGCCGAAAAGGCTCAAGAACAAAGAGTTTGAGTGATTTTATCGAAACCCCTTTCGGTTTATCTCCTTTGAAGTGTTCTTCGGATTCTCTTGATTCTTATAAGCATCAAGGGTTTAACAATCCTCTTTTCGAAGCCTCCGGTGAATCGGAGTTCAACCGGTTTAGATCTTCGCCGCCTCCGAAGTTCAAGTTTTTGAGAGACGCGGAAGAGAAGTTGTATAGAAGATTGATGGAAGAATCTAGAAGAAAGGAAGTTGAGGAGAATCATGAATATGAGGTTGAAGATTCTCCTAAAAGAGAAACAGAAAGGAAACAAATTCAACAATGTTTGTCACAGAGTTCTTCTCAGGTTTTTCCTTTAACATCTTCTCCTACAATGTTGAAATCACTTGAAAACTCATCTATAGTGCATTAGATCTATATACAATTAGGACTTTGATTAACTTTTTTTTTACCTTTATGTTACATTAATTTTAGTAATGGACCTCTATTATGTTTGTTCTTNAATGTATTTGTTATTGGTTAATATGCTGACTAAATTTAAGTT
TARGET=215,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3743
PRIMER_LEFT_PENALTY=1.013951
PRIMER_RIGHT_PENALTY=0.360320
PRIMER_LEFT_SEQUENCE=ACACAACTCATAAGTCATGCCC
PRIMER_RIGHT_SEQUENCE=GGACCATGCAACTTCTTCAAAT
PRIMER_LEFT=81,22
PRIMER_RIGHT=260,22
PRIMER_LEFT_TM=58.986
PRIMER_RIGHT_TM=60.360
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=1.3994
PRIMER_LEFT_1_PENALTY=1.013951
PRIMER_RIGHT_1_PENALTY=0.385416
PRIMER_LEFT_1_SEQUENCE=ACACAACTCATAAGTCATGCCC
PRIMER_RIGHT_1_SEQUENCE=GACCATGCAACTTCTTCAAATG
PRIMER_LEFT_1=81,22
PRIMER_RIGHT_1=259,22
PRIMER_LEFT_1_TM=58.986
PRIMER_RIGHT_1_TM=59.615
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=179
PRIMER_PAIR_PENALTY_2=1.7569
PRIMER_LEFT_2_PENALTY=1.013951
PRIMER_RIGHT_2_PENALTY=0.742980
PRIMER_LEFT_2_SEQUENCE=ACACAACTCATAAGTCATGCCC
PRIMER_RIGHT_2_SEQUENCE=AGGAGGTGATATCCGACAACAA
PRIMER_LEFT_2=81,22
PRIMER_RIGHT_2=327,22
PRIMER_LEFT_2_TM=58.986
PRIMER_RIGHT_2_TM=60.743
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=2.0955
PRIMER_LEFT_3_PENALTY=1.013951
PRIMER_RIGHT_3_PENALTY=1.081536
PRIMER_LEFT_3_SEQUENCE=ACACAACTCATAAGTCATGCCC
PRIMER_RIGHT_3_SEQUENCE=AAAACCACAAAAGGATGGACC
PRIMER_LEFT_3=81,22
PRIMER_RIGHT_3=276,21
PRIMER_LEFT_3_TM=58.986
PRIMER_RIGHT_3_TM=60.082
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=2.1126
PRIMER_LEFT_4_PENALTY=1.013951
PRIMER_RIGHT_4_PENALTY=1.098677
PRIMER_LEFT_4_SEQUENCE=ACACAACTCATAAGTCATGCCC
PRIMER_RIGHT_4_SEQUENCE=GGAGGTGATATCCGACAACAAA
PRIMER_LEFT_4=81,22
PRIMER_RIGHT_4=326,22
PRIMER_LEFT_4_TM=58.986
PRIMER_RIGHT_4_TM=61.099
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_04477 Ps_contig_mira-and-tgicl-ass_franssen_33360
SEQUENCE=GGAAAAGGACGAATTATAATTAAGCCTCTCTCACATACACACCGAAGAGACGAGAAGAGAACGATAAAGAAAGACGAAGTTGTGGCTGTGGTGAACGAGGAATTCAGAATCAAATCGATTTTATTAATTGATCCAAACGCGCTTCTTTCTTCTAGTTCCACTTCTATTTAATTCAATTAGATTCACAAAAAAAGCGCTTCCGACTTTGTTCTTAATTCTCTATTGTCGGTGTTAAGGGTTTTCACTTTCAATGGCAGATGTAGCCGATAAATTGGCGTATTTCCAAGCGATCACCGGTTTGGAAGATGTCGACCTCTGTACTGAGATCCTCGCCGCTCACGGTTGGGACCTCGAACTCGCCATCTCTTCTTTCACCACTACTTCTCACCCAACCACCGCCACCGCCACCGCCGGCGATGATGATGTTGTTGATGTTAATGTTAATAATAACAATCTACAATCATCTTCCCAATCGGAACTGCCTCTTCCTCCGCCACCTCCCGGATTGGCTTGGAAAATAATCAAATTACCCGTCTCTGTGATTTCTGGTAGTCTAGGGTTAATTTCTGGAGCAGTTGGTTTGGGATTATGGGCTGCTGGTGGTGTTCTTTCGTATTCGTTGGGGTTCATGGGCTTGGGCCCGGGCTCTGCGACTTCCTCTTCATCTGCTTCGGCGCCGTTGGTTTCTGTGTCGGCGGCGACCACTGAAGCCATGAATTTTGTTGCGGCTTTTGAGAGGGATTATGGAACGACGAAACCTAATTTTGTGGGTGAGGGTTTCATGGACGCGCTTCAGAGGTCGAGGAATTCGTTTAAGCTTCTGTTTGTTTACTTGCATTCTCCTGATCATCCTGATACGCCGTTGTTTTGTCGGAGGACGCTTTGTTCTGATGGGTTTGTTGAGTTTGTGAATCAGAATTTTGTATGCTGGGGTGGTAGTATTCGGGCTAGTGAAGGATTTAAGATGAGTAATAGTTTGAAGGCTTCTCGATTTTCCTTTTTGTGCTCTTGTTATGGCTGCAACTAATCAGAGAATTGCACTCCTTCAACAGGTTGAAGGTCCCAAATCCCCCGAGGAGATGTTAGTGACACTTCAGAGAGTGCTTGAAGAAAGTTCCCCAGTTCTTGTTGCAGCAAGGCTTGATGCAGAAGAAAGACGAAATAATGTACGCTTAAGGGAAGAGCAAGATGCTGCATACAGGGCTGCACTAGAAGCTGATCAAGCTAGGGAACGGCAGAGAAGAGAAGAAGAAGAGCGTCTTGCAAGGGAAGCTGCTGAAGCTGAGAGAAAGCTTAAGGAAGAAGAGGAGGCTCGTGAAAGAGCAGCACGGGAAGCTGCCGAGAAACAAGCAGCATTAGCGAAATTACGTGAAGAGAAAGCTCAGTCTCTTGGTGAAGAACCTGAAAAAGGACCGAATGTTACACAGGTTTTGGTACGGTTTCCAAATGGTGTACGCAAGGAAAGGAGGTTCAACAGCACTGTGACAATTCAATCATTGTATGACTATGTTGATTCATTGGGTTGTTTAGAAGTCGAGAATTACAGCCTAGTCTCTAATTTTCCTCGGGTTGTTTATGGACAAGAAAAGTTGACACTGTCATTAAAAGAAGCAGGATTGCATCCTCAGGCCAGCCTATTTGTGGAGCTCAGTTCATGAGGATAGAACTTGCCAAATAATCTTTTGCATACGTGAAAATGTGACCTGCAGATAGGGGACTCTGTTTTTTCACTTGTCTAGTTTTAAAAAAAAATTTACTTGAACTTTATAATGTTAGACATAGAATAATAGATCACTGTGGTTAATGTAGTTTTTGGTTTCTCTTGTTTTCCATTGAATCAAGCATATAGCTTTACATGGAGTTGGTTTAGTATAATGTTACATAATACACTTTGTTTTCCGTATNAAAAATAGTATTGCAGAATTTCAGT
TARGET=1244,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1767
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.151106
PRIMER_LEFT_SEQUENCE=AGGCTTGATGCAGAAGAAAGAC
PRIMER_RIGHT_SEQUENCE=AAACCTGTGTAACATTCGGTCC
PRIMER_LEFT=1136,22
PRIMER_RIGHT=1430,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=60.151
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=0.2308
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.205226
PRIMER_LEFT_1_SEQUENCE=AGGCTTGATGCAGAAGAAAGAC
PRIMER_RIGHT_1_SEQUENCE=AACCTGTGTAACATTCGGTCCT
PRIMER_LEFT_1=1136,22
PRIMER_RIGHT_1=1429,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=59.795
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=0.2908
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=0.278625
PRIMER_LEFT_2_SEQUENCE=GAAAGTTCCCCAGTTCTTGTTG
PRIMER_RIGHT_2_SEQUENCE=TCACGTAATTTCGCTAATGCTG
PRIMER_LEFT_2=1109,22
PRIMER_RIGHT_2=1371,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.279
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=0.3042
PRIMER_LEFT_3_PENALTY=0.025566
PRIMER_RIGHT_3_PENALTY=0.278625
PRIMER_LEFT_3_SEQUENCE=AGGCTTGATGCAGAAGAAAGAC
PRIMER_RIGHT_3_SEQUENCE=TCACGTAATTTCGCTAATGCTG
PRIMER_LEFT_3=1136,22
PRIMER_RIGHT_3=1371,22
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=60.279
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.3088
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=0.296664
PRIMER_LEFT_4_SEQUENCE=GAAAGTTCCCCAGTTCTTGTTG
PRIMER_RIGHT_4_SEQUENCE=ACGTAATTTCGCTAATGCTGCT
PRIMER_LEFT_4=1109,22
PRIMER_RIGHT_4=1369,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=60.297
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=261
=
PRIMER_SEQUENCE_ID=SSR_04478 Ps_contig_mira-and-tgicl-ass_franssen_33364
SEQUENCE=CGGTTATCGTTTTTCGTACGCCGCCGAGGCTCTTACAATTAGTGACAGCGTCTTTGCCACGGAATCTCAGGGCGGCGATATCGTAGGCTCTGGCGGCTTCGTCTTCTTCATTGAACGTACCAAGCCATACGCGCTGATGTTTCTCGTAAATCTGAGCTCCCCAACGTCCATTCGGCTGTGGCACCACGCCTTTGTATTTCGACGACGGGAGTTTACGAGATTCAGCTTCGCCGGAGCCGACACAGTTTTCAGGATCGATAACGGCGCTAGCACCGCTTCCAACGCGACTGAGTGTGTTCATTTGCGGCTGTGGAGATAGTTTCGCCGGAACAAGAGATACCGAAAGAGAGTCATTGCTTGTGGTGGTTTCATCTGTGCAACTACCTAACTCCATTTTTTTTGTTATCTATTTTAAACTAACTTTGATTTGTTTTTCTGTTTATAACTAACTATTTAGTTAGTTAGAAAAAAAAGTAGAAGAAATAGTGAAAGATGAACGCAAAGGGGAAGAAGAAGAATGAGAGAATAGGTGGTAGGTGTTGTCCCGCGTACTCTGCGTTGA
TARGET=454,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3390
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.301624
PRIMER_LEFT_SEQUENCE=TGGTTTCATCTGTGCAACTACC
PRIMER_RIGHT_SEQUENCE=TCATTCTTCTTCTTCCCCTTTG
PRIMER_LEFT=363,22
PRIMER_RIGHT=520,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.698
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=0.3538
PRIMER_LEFT_1_PENALTY=0.052187
PRIMER_RIGHT_1_PENALTY=0.301624
PRIMER_LEFT_1_SEQUENCE=CGAAAGAGAGTCATTGCTTGTG
PRIMER_RIGHT_1_SEQUENCE=TCATTCTTCTTCTTCCCCTTTG
PRIMER_LEFT_1=340,22
PRIMER_RIGHT_1=520,22
PRIMER_LEFT_1_TM=60.052
PRIMER_RIGHT_1_TM=59.698
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=0.6354
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.598109
PRIMER_LEFT_2_SEQUENCE=TGGTTTCATCTGTGCAACTACC
PRIMER_RIGHT_2_SEQUENCE=CCTTTGCGTTCATCTTTCACTA
PRIMER_LEFT_2=363,22
PRIMER_RIGHT_2=504,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=59.402
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.6462
PRIMER_LEFT_3_PENALTY=0.048131
PRIMER_RIGHT_3_PENALTY=0.598109
PRIMER_LEFT_3_SEQUENCE=AGTTTACGAGATTCAGCTTCGC
PRIMER_RIGHT_3_SEQUENCE=CCTTTGCGTTCATCTTTCACTA
PRIMER_LEFT_3=209,22
PRIMER_RIGHT_3=504,22
PRIMER_LEFT_3_TM=60.048
PRIMER_RIGHT_3_TM=59.402
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.6503
PRIMER_LEFT_4_PENALTY=0.052187
PRIMER_RIGHT_4_PENALTY=0.598109
PRIMER_LEFT_4_SEQUENCE=CGAAAGAGAGTCATTGCTTGTG
PRIMER_RIGHT_4_SEQUENCE=CCTTTGCGTTCATCTTTCACTA
PRIMER_LEFT_4=340,22
PRIMER_RIGHT_4=504,22
PRIMER_LEFT_4_TM=60.052
PRIMER_RIGHT_4_TM=59.402
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_04479 Ps_contig_mira-and-tgicl-ass_franssen_33364
SEQUENCE=CGGTTATCGTTTTTCGTACGCCGCCGAGGCTCTTACAATTAGTGACAGCGTCTTTGCCACGGAATCTCAGGGCGGCGATATCGTAGGCTCTGGCGGCTTCGTCTTCTTCATTGAACGTACCAAGCCATACGCGCTGATGTTTCTCGTAAATCTGAGCTCCCCAACGTCCATTCGGCTGTGGCACCACGCCTTTGTATTTCGACGACGGGAGTTTACGAGATTCAGCTTCGCCGGAGCCGACACAGTTTTCAGGATCGATAACGGCGCTAGCACCGCTTCCAACGCGACTGAGTGTGTTCATTTGCGGCTGTGGAGATAGTTTCGCCGGAACAAGAGATACCGAAAGAGAGTCATTGCTTGTGGTGGTTTCATCTGTGCAACTACCTAACTCCATTTTTTTTGTTATCTATTTTAAACTAACTTTGATTTGTTTTTCTGTTTATAACTAACTATTTAGTTAGTTAGAAAAAAAAGTAGAAGAAATAGTGAAAGATGAACGCAAAGGGGAAGAAGAAGAATGAGAGAATAGGTGGTAGGTGTTGTCCCGCGTACTCTGCGTTGA
TARGET=507,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8903
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.852926
PRIMER_LEFT_SEQUENCE=TGGTTTCATCTGTGCAACTACC
PRIMER_RIGHT_SEQUENCE=GGGACAACACCTACCACCTATT
PRIMER_LEFT=363,22
PRIMER_RIGHT=545,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.147
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.9051
PRIMER_LEFT_1_PENALTY=0.052187
PRIMER_RIGHT_1_PENALTY=0.852926
PRIMER_LEFT_1_SEQUENCE=CGAAAGAGAGTCATTGCTTGTG
PRIMER_RIGHT_1_SEQUENCE=GGGACAACACCTACCACCTATT
PRIMER_LEFT_1=340,22
PRIMER_RIGHT_1=545,22
PRIMER_LEFT_1_TM=60.052
PRIMER_RIGHT_1_TM=59.147
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=1.4563
PRIMER_LEFT_2_PENALTY=0.603423
PRIMER_RIGHT_2_PENALTY=0.852926
PRIMER_LEFT_2_SEQUENCE=CGGAACAAGAGATACCGAAAGA
PRIMER_RIGHT_2_SEQUENCE=GGGACAACACCTACCACCTATT
PRIMER_LEFT_2=325,22
PRIMER_RIGHT_2=545,22
PRIMER_LEFT_2_TM=60.603
PRIMER_RIGHT_2_TM=59.147
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=221
PRIMER_PAIR_PENALTY_3=1.6352
PRIMER_LEFT_3_PENALTY=0.782312
PRIMER_RIGHT_3_PENALTY=0.852926
PRIMER_LEFT_3_SEQUENCE=ATTGCTTGTGGTGGTTTCATCT
PRIMER_RIGHT_3_SEQUENCE=GGGACAACACCTACCACCTATT
PRIMER_LEFT_3=352,22
PRIMER_RIGHT_3=545,22
PRIMER_LEFT_3_TM=60.782
PRIMER_RIGHT_3_TM=59.147
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=1.6451
PRIMER_LEFT_4_PENALTY=0.792166
PRIMER_RIGHT_4_PENALTY=0.852926
PRIMER_LEFT_4_SEQUENCE=ACCGAAAGAGAGTCATTGCTTG
PRIMER_RIGHT_4_SEQUENCE=GGGACAACACCTACCACCTATT
PRIMER_LEFT_4=338,22
PRIMER_RIGHT_4=545,22
PRIMER_LEFT_4_TM=60.792
PRIMER_RIGHT_4_TM=59.147
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_04480 Ps_contig_mira-and-tgicl-ass_franssen_33367
SEQUENCE=AATCAGATGCCAGTACCAGAGATAGACTTTTGGCTAGTTTTAAATCCCCTGGCCAGGCTTGGGTGGAAGCTTTGGATTGGTGCAGCTGTTTTCTCGTGGGGATGGATTCACCAATATCGGTGCCATAAGATTCTCGGTTCTCTGCGTGATTCAAGACAGGCCGAAGAATATGTAATTCCTCGCGGTGATTGGTTTGAATTGTTTCCTCTCCGCACTATCTCTCTGAAATTGTAATCTACGCCAGTTTTGTGGTTGCTACTGGTGGATCCAATCTAACAATCTGGCTACTTTTTGCTTTTGTGGTGGCAAATCTGGCATTTGCAGCAGTAGAAACACATAGCTGGTATCGTCGTAAATTTGAAGATTATCCAAGCCGTCGGTTTGCGATTATTCCATTTATTCTCTGAGATTGTGAGGCAAAGTGTAACTATCTAAAAATATGACAAGTAAATATCTATATCTTCTCAAGATGTACATATTTAGGTGAAACTGTTTTATTTATTTATGAAAAAAGACTTAGTATTAACT
TARGET=494,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04481 Ps_contig_mira-and-tgicl-ass_franssen_33372
SEQUENCE=CCNAGGACCTAAACAACTACATTAACATAAGAGAATCCAAGATTACAATTAGAAACATATGACTTTGACTTTGATTCAAAGGCATTTTTAGAAATCAGGTATCTGGATTTTATTTTCATCATCAACAAACATGGTTCCTCACTCTACATTATGAAAGGAAGATACAAAAAGTTGAGCTTTTTTCCCATCATTTTTAAATAGGGAAATGAAATGGTGATAGTACATGCAAAACAGAGAAAAGTGATTCACGATCAAATATCCAAAAAATCTTAAGGCCAAGCATCTGGAGAGACTTTTTCACTGATTGTAGAACCAAATTGTGGTCCTCCTTTCCTATATTGAATAAGATACTCAATGGGGTATCCCTTAAGCTTATGAGGAACAACTCCAAGATCATTGAATGTCAAAGCATTTCCTGAAACAACATTATCTGTAGCAAAAGCATGGATCTGATCCAGATTGAACGTCTCAGGCTTGGGCAGTGGAAAGGGGACCTTGTTTAATAATAATTCCCTTGGTGTTGCCAATGCCTTCGCAATGGGAAGAGGCACATTTACATATCGAGGCCATTCTCGAATCACATCATACATAATCTCTGCCAATTCGTGAATGGTAAAGATCTCGGGGCCACCCAACTCATAAGTCTTTCCCATGCTAGTTCCATCATCCTTCAAGGCTGTAGTAAGTGCAGAGGCGACATCAACAACATATACAGGTTGGATTTTTGTGTTGGCGTTCCCCATCAATGGAAGAAAGCCATATTTCTTTGCAAATTGAGCCCATCGGTTTAAAATCCGGTCCTCTGTGCCAATCATAGCAGCAGGTTTGAAAATTGTGGCCTCAGGTAATTCTCTTAAAACTGCTTCTTCGGCAGCTGCTTTACATCTCAGCATCCTGGAAGAAGATGAAGGAGATGCACCTAAACAAGAAACTTGAATGAATCTCATGATACCACCATGCTCTTTGGAAATCTTTGCAAGTTTCTCGGCCATGTGATAGTGCACTTCTTCATAGCTGTAGTTTCTTGTCTCATAATCCCTTCCAATAAGATTGATGACAACATTGGCCCTAGCCATTACAGCCTTAACCGAACTTTCGTCTCTTGGATTGAATTTCATGGGTACAATCTGTCCTAAATCTCCCATCAGTTTGAGATGACGAGGACAATCTTCAGAGCCTCGGAAAGGAACTAAGACTTGAGATCCCATTTTAGCAAGTTGTTGGACAACATAGCGACCAAGGAATCCAGTTGCCCCAAATACTGTAGCAACAATACCACTAACAGATGATCGTCCACCAGTACCCTTGCGGACGAGGTGGCCAACGCCCTTGGTAGCTATGGTTGATACGTAACGTTCGTGATCCACACCATAATGGTGATGGGAAATTGGGTAGATGGATTTTAGCGAAGTTGAGGATTGGGAGCCTAAGCGTCTTGTAATGGCTTGCATCTTAGCGTTCCCAGAAAACGCAGAACAATCGAAGCAGTATGTGAGAGAGAGAGAGAGAGAGAGACTGTTACGCTATCNCCCGCGTACTCTGTCGTTGACTACCACTGTCTTCTAG
TARGET=1494,22
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8139
PRIMER_LEFT_PENALTY=0.129103
PRIMER_RIGHT_PENALTY=1.684803
PRIMER_LEFT_SEQUENCE=CATCTTAGCGTTCCCAGAAAAC
PRIMER_RIGHT_SEQUENCE=GAAGACAGTGGTAGTCAACGACA
PRIMER_LEFT=1448,22
PRIMER_RIGHT=1562,23
PRIMER_LEFT_TM=60.129
PRIMER_RIGHT_TM=59.315
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=1.8713
PRIMER_LEFT_1_PENALTY=0.186529
PRIMER_RIGHT_1_PENALTY=1.684803
PRIMER_LEFT_1_SEQUENCE=CCTAAGCGTCTTGTAATGGCTT
PRIMER_RIGHT_1_SEQUENCE=GAAGACAGTGGTAGTCAACGACA
PRIMER_LEFT_1=1425,22
PRIMER_RIGHT_1=1562,23
PRIMER_LEFT_1_TM=59.813
PRIMER_RIGHT_1_TM=59.315
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=1.9372
PRIMER_LEFT_2_PENALTY=0.252355
PRIMER_RIGHT_2_PENALTY=1.684803
PRIMER_LEFT_2_SEQUENCE=GGGAAATTGGGTAGATGGATTT
PRIMER_RIGHT_2_SEQUENCE=GAAGACAGTGGTAGTCAACGACA
PRIMER_LEFT_2=1381,22
PRIMER_RIGHT_2=1562,23
PRIMER_LEFT_2_TM=60.252
PRIMER_RIGHT_2_TM=59.315
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=182
PRIMER_PAIR_PENALTY_3=1.9610
PRIMER_LEFT_3_PENALTY=0.276218
PRIMER_RIGHT_3_PENALTY=1.684803
PRIMER_LEFT_3_SEQUENCE=GAGCCTAAGCGTCTTGTAATGG
PRIMER_RIGHT_3_SEQUENCE=GAAGACAGTGGTAGTCAACGACA
PRIMER_LEFT_3=1422,22
PRIMER_RIGHT_3=1562,23
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.315
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=141
PRIMER_PAIR_PENALTY_4=1.9610
PRIMER_LEFT_4_PENALTY=0.276218
PRIMER_RIGHT_4_PENALTY=1.684803
PRIMER_LEFT_4_SEQUENCE=GGAGCCTAAGCGTCTTGTAATG
PRIMER_RIGHT_4_SEQUENCE=GAAGACAGTGGTAGTCAACGACA
PRIMER_LEFT_4=1421,22
PRIMER_RIGHT_4=1562,23
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=59.315
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=142
=
PRIMER_SEQUENCE_ID=SSR_04482 Ps_contig_mira-and-tgicl-ass_franssen_33383
SEQUENCE=AATTCCAATCCGGCGGGTGTAATAACAAGCTAATACCGGAAACTAAAGTCTTGACCTAGCGACGCAGCCATCACTCACTCTCTGCCTTCTGAATCGCACCAACATGGACATCAAAACCCTTTCCTTCGCCGCATCCACGCCACCGTTAGCTCTCATCGCCGCCGCCAAGCTTGCCGGAATTTCTCCATCCATCAATTCCTCCCTCCCTCCCGACCTCCCGCCCCCACACTTCACTTCTCCAACGGGTCAAAATTGCGTGGAGCATTTGTTCTTCTCCGTTATATTGGAAGAACTTCCACACTTCCGAATTTCTATGGCCAAAATGCATTTGAAACTAGCCAGATTGATGAATTGTTAGAGTATGCACCTGTACTATCATCTGGCCCTGCTTTTGAGAATGGATGCAAATATATTGACGAGTACTTGGAGAAGCGTACATTTTTAGTTGGTTACTCATTATCAATTGCAGATATAGCTATCTGGGCAGGTCTTGCAGGGGCTGGAAAAAGATGGGAAAAGTTTGAGGAAGTCAAAGAAATACCAAAATCTTGCGCGATGGTACAATTCAATAGTGACAGAACACGGTACTTCCTTAAACGTTAAGTTCTACGTACTAACTATTATTGTTTGGTTAAAAAAGGATTGGGAGAGCCATCTGCAACCAAGACAAAGGACCAGGCAGTCATCACAGATAAAGTAAGGAATGTGAATGGGGATGCATCTGATAACATTAAGGGAGGAGGCAAACCTTTAGCTGAGATAGATCTTCCAGATGCTGAGGTTGGAAAAGTCCGCTTGCGATTTGCACCTGAACCCAGTGGCTATCTTCACATTGGACACTCAAAAGCAGCTCTGTTGAACAAGTATTTTGCTGAGCGATACCAGGGTCAGGTTATTATTCGTTTTGATGATACCAATCCTGCTAAAGAAAGTAATGAATTTGTAGACAACTTGGTGAAAGATATTGATACATTGGGTATCAAATATGAAACAATTACATATAACGTACAAGAAAATTACTTCCCTGAGTTAATGGAATTGGCTG
TARGET=198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2113
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.115769
PRIMER_LEFT_SEQUENCE=ACATGGACATCAAAACCCTTTC
PRIMER_RIGHT_SEQUENCE=AAAGCAGGGCCAGATGATAGTA
PRIMER_LEFT=101,22
PRIMER_RIGHT=391,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=60.116
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=4.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.5914
PRIMER_LEFT_1_PENALTY=0.095574
PRIMER_RIGHT_1_PENALTY=0.495821
PRIMER_LEFT_1_SEQUENCE=ACATGGACATCAAAACCCTTTC
PRIMER_RIGHT_1_SEQUENCE=GAAATTCGGAAGTGTGGAAGTT
PRIMER_LEFT_1=101,22
PRIMER_RIGHT_1=311,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.504
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.8079
PRIMER_LEFT_2_PENALTY=0.095574
PRIMER_RIGHT_2_PENALTY=0.712323
PRIMER_LEFT_2_SEQUENCE=ACATGGACATCAAAACCCTTTC
PRIMER_RIGHT_2_SEQUENCE=CCATAGAAATTCGGAAGTGTGG
PRIMER_LEFT_2=101,22
PRIMER_RIGHT_2=316,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.712
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=216
PRIMER_PAIR_PENALTY_3=0.8583
PRIMER_LEFT_3_PENALTY=0.095574
PRIMER_RIGHT_3_PENALTY=0.762694
PRIMER_LEFT_3_SEQUENCE=ACATGGACATCAAAACCCTTTC
PRIMER_RIGHT_3_SEQUENCE=GGGCCAGATGATAGTACAGGTG
PRIMER_LEFT_3=101,22
PRIMER_RIGHT_3=385,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=60.763
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.9448
PRIMER_LEFT_4_PENALTY=0.095574
PRIMER_RIGHT_4_PENALTY=0.849238
PRIMER_LEFT_4_SEQUENCE=ACATGGACATCAAAACCCTTTC
PRIMER_RIGHT_4_SEQUENCE=GCCATAGAAATTCGGAAGTGTG
PRIMER_LEFT_4=101,22
PRIMER_RIGHT_4=317,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=60.849
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_04483 Ps_contig_mira-and-tgicl-ass_franssen_33384
SEQUENCE=AAAGTAAAGTACGTTTACCTAACCAACNGANATTACCTTCCGACACANAACGTAAGTCGCTTCTTCTGCGAAGACTGCTTCCCAGAGGAATTATCATCAATTTCTTCATCAACACTTCTTTTTAATGAAAGTAGTTATACTCTCAGCATGGTTCTTGTGGATAATTCCAACAGAACTCTGTAGATACTCCACCATCTGGTCACTAGATTTCCCAAAGAACTTATCTTTGTAGAGTTGTTTGAGCTGAGCAACACCTTGGATGCCTTTTTGAACTAGTTTTCTGAAATTTCTGGGACCCACACCGGGAATAGCCAACAAATCAGGATTTGACGATGGAGAAGAAGAAGAAGAAACCTTTTGCCTCCTATTTAGTCTGACGTCGGCGGTAGATGATGAAGAAGAAGAAGTTGTATCCTGGTGTTGTTGTTCAGAGGATGAATGATGTAGAGAGGAATTTGAATATAGAGAGCGGTTAAGAAGAAGCCATGGAAGGGTTTTGGTGGGGGTTAGGGTTAGAGTGGGAGAGAAAGATGTACGGAGATAGGAGTAACGAGGAGAAGAATGTTTGAGAAGGAGAGTGTTATTGTTGTTTGGGAATGAAATTCTAATGGATGACGGCGTTGTTGTTGCTGCTGCTGTAGACATTGTTGTTGGGAGATGGAGAAAGAGAGGGTTAGAGGAAGAGGAAGAAGAAGAAGAAGATAGAATAGGGATTGGGAGTGAACAAGTTGGACGCTGAAGAAGTTTCTGCATTATTATTATTATTCGTTACTACTTGTTCTTCTTCTAAGCGGAAGCATTTGTGGTTACTTGTTACTACAACAACGTTGTTCCGTTGGGTCTAAGAACAAGTATTCATTNTTCCCCGCGTACT
TARGET=337,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1447
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=0.012470
PRIMER_LEFT_SEQUENCE=CTCAGCATGGTTCTTGTGGATA
PRIMER_RIGHT_SEQUENCE=TCATCCTCTGAACAACAACACC
PRIMER_LEFT=141,22
PRIMER_RIGHT=437,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.4223
PRIMER_LEFT_1_PENALTY=0.409822
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=AGTTGTTTGAGCTGAGCAACAC
PRIMER_RIGHT_1_SEQUENCE=TCATCCTCTGAACAACAACACC
PRIMER_LEFT_1=232,22
PRIMER_RIGHT_1=437,22
PRIMER_LEFT_1_TM=59.590
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=0.4747
PRIMER_LEFT_2_PENALTY=0.462203
PRIMER_RIGHT_2_PENALTY=0.012470
PRIMER_LEFT_2_SEQUENCE=CCTTGGATGCCTTTTTGAACTA
PRIMER_RIGHT_2_SEQUENCE=TCATCCTCTGAACAACAACACC
PRIMER_LEFT_2=253,22
PRIMER_RIGHT_2=437,22
PRIMER_LEFT_2_TM=60.462
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.7141
PRIMER_LEFT_3_PENALTY=0.132262
PRIMER_RIGHT_3_PENALTY=0.581808
PRIMER_LEFT_3_SEQUENCE=CTCAGCATGGTTCTTGTGGATA
PRIMER_RIGHT_3_SEQUENCE=TTCTTCTTCATCATCTACCGCC
PRIMER_LEFT_3=141,22
PRIMER_RIGHT_3=402,22
PRIMER_LEFT_3_TM=60.132
PRIMER_RIGHT_3_TM=60.582
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=12.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.7494
PRIMER_LEFT_4_PENALTY=0.462203
PRIMER_RIGHT_4_PENALTY=0.287182
PRIMER_LEFT_4_SEQUENCE=CCTTGGATGCCTTTTTGAACTA
PRIMER_RIGHT_4_SEQUENCE=TCTCCGTACATCTTTCTCTCCC
PRIMER_LEFT_4=253,22
PRIMER_RIGHT_4=540,22
PRIMER_LEFT_4_TM=60.462
PRIMER_RIGHT_4_TM=59.713
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_04484 Ps_contig_mira-and-tgicl-ass_franssen_33384
SEQUENCE=AAAGTAAAGTACGTTTACCTAACCAACNGANATTACCTTCCGACACANAACGTAAGTCGCTTCTTCTGCGAAGACTGCTTCCCAGAGGAATTATCATCAATTTCTTCATCAACACTTCTTTTTAATGAAAGTAGTTATACTCTCAGCATGGTTCTTGTGGATAATTCCAACAGAACTCTGTAGATACTCCACCATCTGGTCACTAGATTTCCCAAAGAACTTATCTTTGTAGAGTTGTTTGAGCTGAGCAACACCTTGGATGCCTTTTTGAACTAGTTTTCTGAAATTTCTGGGACCCACACCGGGAATAGCCAACAAATCAGGATTTGACGATGGAGAAGAAGAAGAAGAAACCTTTTGCCTCCTATTTAGTCTGACGTCGGCGGTAGATGATGAAGAAGAAGAAGTTGTATCCTGGTGTTGTTGTTCAGAGGATGAATGATGTAGAGAGGAATTTGAATATAGAGAGCGGTTAAGAAGAAGCCATGGAAGGGTTTTGGTGGGGGTTAGGGTTAGAGTGGGAGAGAAAGATGTACGGAGATAGGAGTAACGAGGAGAAGAATGTTTGAGAAGGAGAGTGTTATTGTTGTTTGGGAATGAAATTCTAATGGATGACGGCGTTGTTGTTGCTGCTGCTGTAGACATTGTTGTTGGGAGATGGAGAAAGAGAGGGTTAGAGGAAGAGGAAGAAGAAGAAGAAGATAGAATAGGGATTGGGAGTGAACAAGTTGGACGCTGAAGAAGTTTCTGCATTATTATTATTATTCGTTACTACTTGTTCTTCTTCTAAGCGGAAGCATTTGTGGTTACTTGTTACTACAACAACGTTGTTCCGTTGGGTCTAAGAACAAGTATTCATTNTTCCCCGCGTACT
TARGET=395,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0869
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=0.012470
PRIMER_LEFT_SEQUENCE=AGGATTTGACGATGGAGAAGAA
PRIMER_RIGHT_SEQUENCE=TCATCCTCTGAACAACAACACC
PRIMER_LEFT=321,22
PRIMER_RIGHT=437,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=117
PRIMER_PAIR_PENALTY_1=0.0869
PRIMER_LEFT_1_PENALTY=0.074466
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=GGATTTGACGATGGAGAAGAAG
PRIMER_RIGHT_1_SEQUENCE=TCATCCTCTGAACAACAACACC
PRIMER_LEFT_1=322,22
PRIMER_RIGHT_1=437,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=116
PRIMER_PAIR_PENALTY_2=0.1447
PRIMER_LEFT_2_PENALTY=0.132262
PRIMER_RIGHT_2_PENALTY=0.012470
PRIMER_LEFT_2_SEQUENCE=CTCAGCATGGTTCTTGTGGATA
PRIMER_RIGHT_2_SEQUENCE=TCATCCTCTGAACAACAACACC
PRIMER_LEFT_2=141,22
PRIMER_RIGHT_2=437,22
PRIMER_LEFT_2_TM=60.132
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=0.3321
PRIMER_LEFT_3_PENALTY=0.319597
PRIMER_RIGHT_3_PENALTY=0.012470
PRIMER_LEFT_3_SEQUENCE=AAATCAGGATTTGACGATGGAG
PRIMER_RIGHT_3_SEQUENCE=TCATCCTCTGAACAACAACACC
PRIMER_LEFT_3=316,22
PRIMER_RIGHT_3=437,22
PRIMER_LEFT_3_TM=60.320
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=122
PRIMER_PAIR_PENALTY_4=0.4223
PRIMER_LEFT_4_PENALTY=0.409822
PRIMER_RIGHT_4_PENALTY=0.012470
PRIMER_LEFT_4_SEQUENCE=AGTTGTTTGAGCTGAGCAACAC
PRIMER_RIGHT_4_SEQUENCE=TCATCCTCTGAACAACAACACC
PRIMER_LEFT_4=232,22
PRIMER_RIGHT_4=437,22
PRIMER_LEFT_4_TM=59.590
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=206
=
PRIMER_SEQUENCE_ID=SSR_04485 Ps_contig_mira-and-tgicl-ass_franssen_33384
SEQUENCE=AAAGTAAAGTACGTTTACCTAACCAACNGANATTACCTTCCGACACANAACGTAAGTCGCTTCTTCTGCGAAGACTGCTTCCCAGAGGAATTATCATCAATTTCTTCATCAACACTTCTTTTTAATGAAAGTAGTTATACTCTCAGCATGGTTCTTGTGGATAATTCCAACAGAACTCTGTAGATACTCCACCATCTGGTCACTAGATTTCCCAAAGAACTTATCTTTGTAGAGTTGTTTGAGCTGAGCAACACCTTGGATGCCTTTTTGAACTAGTTTTCTGAAATTTCTGGGACCCACACCGGGAATAGCCAACAAATCAGGATTTGACGATGGAGAAGAAGAAGAAGAAACCTTTTGCCTCCTATTTAGTCTGACGTCGGCGGTAGATGATGAAGAAGAAGAAGTTGTATCCTGGTGTTGTTGTTCAGAGGATGAATGATGTAGAGAGGAATTTGAATATAGAGAGCGGTTAAGAAGAAGCCATGGAAGGGTTTTGGTGGGGGTTAGGGTTAGAGTGGGAGAGAAAGATGTACGGAGATAGGAGTAACGAGGAGAAGAATGTTTGAGAAGGAGAGTGTTATTGTTGTTTGGGAATGAAATTCTAATGGATGACGGCGTTGTTGTTGCTGCTGCTGTAGACATTGTTGTTGGGAGATGGAGAAAGAGAGGGTTAGAGGAAGAGGAAGAAGAAGAAGAAGATAGAATAGGGATTGGGAGTGAACAAGTTGGACGCTGAAGAAGTTTCTGCATTATTATTATTATTCGTTACTACTTGTTCTTCTTCTAAGCGGAAGCATTTGTGGTTACTTGTTACTACAACAACGTTGTTCCGTTGGGTCTAAGAACAAGTATTCATTNTTCCCCGCGTACT
TARGET=686,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0705
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.033137
PRIMER_LEFT_SEQUENCE=GCTGTAGACATTGTTGTTGGGA
PRIMER_RIGHT_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_LEFT=634,22
PRIMER_RIGHT=852,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.1078
PRIMER_LEFT_1_PENALTY=0.074695
PRIMER_RIGHT_1_PENALTY=0.033137
PRIMER_LEFT_1_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_RIGHT_1_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_LEFT_1=653,22
PRIMER_RIGHT_1=852,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=60.033
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.1090
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.071684
PRIMER_LEFT_2_SEQUENCE=GCTGTAGACATTGTTGTTGGGA
PRIMER_RIGHT_2_SEQUENCE=GAAACTTCTTCAGCGTCCAACT
PRIMER_LEFT_2=634,22
PRIMER_RIGHT_2=747,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=59.928
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=0.1865
PRIMER_LEFT_3_PENALTY=0.037339
PRIMER_RIGHT_3_PENALTY=0.149184
PRIMER_LEFT_3_SEQUENCE=GCTGTAGACATTGTTGTTGGGA
PRIMER_RIGHT_3_SEQUENCE=GCTTCCGCTTAGAAGAAGAACA
PRIMER_LEFT_3=634,22
PRIMER_RIGHT_3=797,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.2239
PRIMER_LEFT_4_PENALTY=0.074695
PRIMER_RIGHT_4_PENALTY=0.149184
PRIMER_LEFT_4_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_RIGHT_4_SEQUENCE=GCTTCCGCTTAGAAGAAGAACA
PRIMER_LEFT_4=653,22
PRIMER_RIGHT_4=797,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=60.149
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_04486 Ps_contig_mira-and-tgicl-ass_franssen_33384
SEQUENCE=AAAGTAAAGTACGTTTACCTAACCAACNGANATTACCTTCCGACACANAACGTAAGTCGCTTCTTCTGCGAAGACTGCTTCCCAGAGGAATTATCATCAATTTCTTCATCAACACTTCTTTTTAATGAAAGTAGTTATACTCTCAGCATGGTTCTTGTGGATAATTCCAACAGAACTCTGTAGATACTCCACCATCTGGTCACTAGATTTCCCAAAGAACTTATCTTTGTAGAGTTGTTTGAGCTGAGCAACACCTTGGATGCCTTTTTGAACTAGTTTTCTGAAATTTCTGGGACCCACACCGGGAATAGCCAACAAATCAGGATTTGACGATGGAGAAGAAGAAGAAGAAACCTTTTGCCTCCTATTTAGTCTGACGTCGGCGGTAGATGATGAAGAAGAAGAAGTTGTATCCTGGTGTTGTTGTTCAGAGGATGAATGATGTAGAGAGGAATTTGAATATAGAGAGCGGTTAAGAAGAAGCCATGGAAGGGTTTTGGTGGGGGTTAGGGTTAGAGTGGGAGAGAAAGATGTACGGAGATAGGAGTAACGAGGAGAAGAATGTTTGAGAAGGAGAGTGTTATTGTTGTTTGGGAATGAAATTCTAATGGATGACGGCGTTGTTGTTGCTGCTGCTGTAGACATTGTTGTTGGGAGATGGAGAAAGAGAGGGTTAGAGGAAGAGGAAGAAGAAGAAGAAGATAGAATAGGGATTGGGAGTGAACAAGTTGGACGCTGAAGAAGTTTCTGCATTATTATTATTATTCGTTACTACTTGTTCTTCTTCTAAGCGGAAGCATTTGTGGTTACTTGTTACTACAACAACGTTGTTCCGTTGGGTCTAAGAACAAGTATTCATTNTTCCCCGCGTACT
TARGET=752,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0705
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.033137
PRIMER_LEFT_SEQUENCE=GCTGTAGACATTGTTGTTGGGA
PRIMER_RIGHT_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_LEFT=634,22
PRIMER_RIGHT=852,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.1078
PRIMER_LEFT_1_PENALTY=0.074695
PRIMER_RIGHT_1_PENALTY=0.033137
PRIMER_LEFT_1_SEQUENCE=GGAGATGGAGAAAGAGAGGGTT
PRIMER_RIGHT_1_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_LEFT_1=653,22
PRIMER_RIGHT_1=852,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=60.033
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.1692
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.033137
PRIMER_LEFT_2_SEQUENCE=TAGGGATTGGGAGTGAACAAGT
PRIMER_RIGHT_2_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_LEFT_2=707,22
PRIMER_RIGHT_2=852,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.033
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.1865
PRIMER_LEFT_3_PENALTY=0.037339
PRIMER_RIGHT_3_PENALTY=0.149184
PRIMER_LEFT_3_SEQUENCE=GCTGTAGACATTGTTGTTGGGA
PRIMER_RIGHT_3_SEQUENCE=GCTTCCGCTTAGAAGAAGAACA
PRIMER_LEFT_3=634,22
PRIMER_RIGHT_3=797,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.2201
PRIMER_LEFT_4_PENALTY=0.186949
PRIMER_RIGHT_4_PENALTY=0.033137
PRIMER_LEFT_4_SEQUENCE=GAATAGGGATTGGGAGTGAACA
PRIMER_RIGHT_4_SEQUENCE=ACTTGTTCTTAGACCCAACGGA
PRIMER_LEFT_4=704,22
PRIMER_RIGHT_4=852,22
PRIMER_LEFT_4_TM=60.187
PRIMER_RIGHT_4_TM=60.033
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_04487 Ps_contig_mira-and-tgicl-ass_franssen_33390
SEQUENCE=CGGTTTCCCTAAAACGGGTAAGTACTTCGAGGCTCCCAAACTACTCCACCGTGCCACCATAGCTCCGTCTGATGCTTGAATGATCTCCTCTAAGTTCTTCAGTGAATCAATACTCTCTATCTTTGCAATGACTGAAATATCACTGTCACGAGATCTTGCAGCAATATAGCTTTTAAGATGAGTAATAACTTCAGCAGATTTGACAAATGAAATGGCAATAAAATCAACACCCTCAGCAATACCAAAATCAATGTCTAACCAATCCTTCGAAGAGATTGTAGGGAGCATGGCGTTCCTTTCTCGAACTAAACTACCATTCCTCCAGAAAGTGAGATTCGCCCTTGGTAGCAACAGGCCAGGATCAGTACAAAGACACTTCACATCTGGACCTATCTTCTCAATGACCTCAAACCTAACCATTCCCCCATCAACCAAAAGCTCATCCCCAACCTTCACATCTTCAGCAAAACCCTCATAGTTGACATTGATGGTACGTTGTGGAAGTGGGAGAGAAGAATCAAATGCCCTAACACTGAAAGTCCAGATCTGACCATCTTCAGCTTTAGCAGAAGAAGCACCAGCGAGATCACCCATATGAATCTCACTTCCTTCCGTATCCATCATAATCGCAACCGCAAATCCCTTGTCATGGTTAAGTCTTCGAACACGGTCGATAACAGTTTTATGCCACTCTCTAGTACCATGACACATGTTAATTCTAGCAACGTTCATTCCTCCAACAGCAAGAGCTTCGAGCTGTTCAAACCCGCACGTAGCGGGTCCCACAGTGCAAACGAGTTTCGTTCTCCTGGTGCTACGGAAGCCATTCTCTTTTAGCTCTGTTTCGGTGACGGCGTCGACTTCGATTGAGCCTAGATCTGGTGGAACTCCCGGCCCTGCTCCGTTGGAGTCGGAGGCGATTATTTGGGATGTGGAGGAGGCGCGGATGATGGGGAAGCGGCGAGGGGGGAAGGAGAGGGTGGGAGATGGGAGGTGGTTGGTGTGGTGGTGGTGGTGGAAGGTTGTGTGTGGGGGTGTGAAGAGCTGGAGGTGGAGGCGGGAGACTGACATTGTGACTGTGGTGAGATGATTGGGAGAAGGGAGTGAGGTTTTGTTTGGGGGAGGCGGAGATAACAAGGGAAGGGAACAGTTGGGAGTTGAGTTACTTGTCTGCGGAAACCACGAGGTTTTGCAACTT
TARGET=1003,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2800
PRIMER_LEFT_PENALTY=0.267873
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TGAGCCTAGATCTGGTGGAACT
PRIMER_RIGHT_SEQUENCE=CAAACAAAACCTCACTCCCTTC
PRIMER_LEFT=867,22
PRIMER_RIGHT=1117,22
PRIMER_LEFT_TM=60.268
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.3725
PRIMER_LEFT_1_PENALTY=0.360320
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=TCGACTTCGATTGAGCCTAGAT
PRIMER_RIGHT_1_SEQUENCE=CAAACAAAACCTCACTCCCTTC
PRIMER_LEFT_1=856,22
PRIMER_RIGHT_1=1117,22
PRIMER_LEFT_1_TM=60.360
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.4144
PRIMER_LEFT_2_PENALTY=0.334070
PRIMER_RIGHT_2_PENALTY=0.080323
PRIMER_LEFT_2_SEQUENCE=GATTATTTGGGATGTGGAGGAG
PRIMER_RIGHT_2_SEQUENCE=TTTCCGCAGACAAGTAACTCAA
PRIMER_LEFT_2=918,22
PRIMER_RIGHT_2=1178,22
PRIMER_LEFT_2_TM=59.666
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.4505
PRIMER_LEFT_3_PENALTY=0.267873
PRIMER_RIGHT_3_PENALTY=0.182645
PRIMER_LEFT_3_SEQUENCE=TGAGCCTAGATCTGGTGGAACT
PRIMER_RIGHT_3_SEQUENCE=GTTCCCTTCCCTTGTTATCTCC
PRIMER_LEFT_3=867,22
PRIMER_RIGHT_3=1147,22
PRIMER_LEFT_3_TM=60.268
PRIMER_RIGHT_3_TM=60.183
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.5058
PRIMER_LEFT_4_PENALTY=0.334070
PRIMER_RIGHT_4_PENALTY=0.171757
PRIMER_LEFT_4_SEQUENCE=GATTATTTGGGATGTGGAGGAG
PRIMER_RIGHT_4_SEQUENCE=GGTTTCCGCAGACAAGTAACTC
PRIMER_LEFT_4=918,22
PRIMER_RIGHT_4=1180,22
PRIMER_LEFT_4_TM=59.666
PRIMER_RIGHT_4_TM=60.172
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_04488 Ps_contig_mira-and-tgicl-ass_franssen_33398
SEQUENCE=TATGATTTGTCTGAACTGGCGTTCCTCGTCGCGACTGATTGCAAGAGGTTCTTCGACAGCATTTTGAGGTTGCTGATCCTCATCTCCGTAACCTATAGAGTAGATAATAATGCCGACAACAACAGCACCAAACGCCAGATAGTACATCCAATCAACCTTCTCATGGTAARCGAAAATGCGAATTAAGACAGACCACATGTCTGATGTCAGAAGAGACAGATTTAGCATGGTAGAACCATTGATCTTAAGCAAAACCGGGACTAGCGAGTAAAACAAAAACATAGCCACTGAAAATACCAAAAAAAGGAAGTGCTGCCCCGGCCGACCAATGAATAGATTTAAGTTCGTTGCGCTCAAGTACTGTTATTTGAATGGCACTGATGATGCCACCAAAGAGACCTAACATTGCCATGAGCTCTTCTCGATCAGCATTTTTTACTTAAAAATTCCTCACTGACATTACTGAATGCATACAAAGTAGCACCAGCGATAACAAGAATGTCCCCTTTACGAGGATTACTTCCACCTGCACGGTCACCTGCGTGAACATCTGAAAATACAACAAGAACAAGTCCAGCGATGCAAACAATTACACCGGTTATCTTTTTGTATCTATATTTTGTTTTCAAGAAAATCCATGTAAGAAGCATGACGCATGGAATGGACCAGCAGTCAAGTAGCATCACACTTGTAAGTGAGGTGTATTGATATGCCTTCACAACAAGAAAATTTGCTTCTACATCAACCAATCCAAGAAGTATATAATAGTACCATTTTGCCTTGAGAGGTTGTCTTCTGTAGAGTAGAATACTTCCATAGACAATCACCAAGAACACATAATTTAGAAACGACTGTGAAGTTGGAGCATTAATTCCTTTTTTAGCTAATTCAGAAGAGGCGAAACCAGTTCCAGTAATAAGGAGAGAAAGAAACTGCCCTAATCCGAGACCAATTAGCGTCTTCTTCGTCCAATATTTCTTAAAATCCATCATCGTTCTCGTTCAAATTTTGCAGAAACAAAAAGAAAAATGGAATAATGAATGAATAAAAAGGAGAATGAATCAAAGAGTTGGTGTAAACGAATGAATGAATTTGATGATTGAAGAAAGTGTGTGTGAGAAGAGAAAGTGACNGAAG
TARGET=1081,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04489 Ps_contig_mira-and-tgicl-ass_franssen_33405
SEQUENCE=TTTTGTCTTAAATTCTAACTTCAATTTGTAATNAATTTCAAAGGCAAAATCAATTCTAATTGAAAACACTGAACATGTGTGACAATTTCTAGAATCTATTTTGAGGCACTAAACATACACATAGACACTCATACTGCACCAAGAAACTATAAAATCAACCAACAAACAATAAAAAGATTATAAAATTCAACTACTTTGTATTAATTCTTAACATTATGCTGTTAATGGTACAAAATGACACCTTCAAGTTCAAACAAAGCATGACATTTGCTCATAATACTCTTGGAGGTCATACCGTAACTTGACTTAGATTCATCATTACTCTTTCGAAGTTATCACGAGCAACCCCAAAGCACCAATGAGAATATACTTGATGATAGGTTTTTCCGTCATGATGATTGTCACCCAGCCAACATAAGGTAAGAACCCGACAGCTCTACCCATAATGTGGTGCCTTTGCAGCCACAGCTGACCATGAGCATACAGAAGCCTGTCATCGCCGTAATTGTTATCTCCTTTTGTGAGGACATCAACTTCCCCATTATCTCCCCTTTCGTGTACCTTAATTACACGATGAACGATCGGAATCTCTCGTCCATCAACATTAAATACAACAATCTCCCCTGCGCGAATTGGATCCTTACTCATGTGTAAGAACAAGATGTCCCCCCGCTTGAATCCCGGTTCCATACTTCCAGAAAGAACAACAACAACAGGAGATTCACTGCCGGTGATGCACATCAATGCCTTCCATATTATCAGTGCAGACGTAACAATCATGCCAAGACTAACACCCTGAGTGAGGACCTGACGGATCTGAAGAGATTTGATAGAATCCACAGTCTCACCGATCAAACCCATAGCTTCTTTCTTCTTTCTTTCTACCAAACTTCACCACTTTCTTGAGTTAACAAATGGAGAAAGAAACACAAAGACCAAACCAAGGCCAATTTCTACCTCCCCGAC
TARGET=703,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2150
PRIMER_LEFT_PENALTY=0.010077
PRIMER_RIGHT_PENALTY=0.204906
PRIMER_LEFT_SEQUENCE=TCGCCGTAATTGTTATCTCCTT
PRIMER_RIGHT_SEQUENCE=GCATGATTGTTACGTCTGCACT
PRIMER_LEFT=496,22
PRIMER_RIGHT=781,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.205
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.2952
PRIMER_LEFT_1_PENALTY=0.186949
PRIMER_RIGHT_1_PENALTY=0.108206
PRIMER_LEFT_1_SEQUENCE=GAGGACATCAACTTCCCCATTA
PRIMER_RIGHT_1_SEQUENCE=AGGGTGTTAGTCTTGGCATGAT
PRIMER_LEFT_1=522,22
PRIMER_RIGHT_1=796,22
PRIMER_LEFT_1_TM=60.187
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.3050
PRIMER_LEFT_2_PENALTY=0.196811
PRIMER_RIGHT_2_PENALTY=0.108206
PRIMER_LEFT_2_SEQUENCE=CCATTATCTCCCCTTTCGTGTA
PRIMER_RIGHT_2_SEQUENCE=AGGGTGTTAGTCTTGGCATGAT
PRIMER_LEFT_2=538,22
PRIMER_RIGHT_2=796,22
PRIMER_LEFT_2_TM=60.197
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.3455
PRIMER_LEFT_3_PENALTY=0.140609
PRIMER_RIGHT_3_PENALTY=0.204906
PRIMER_LEFT_3_SEQUENCE=TGTCATCGCCGTAATTGTTATC
PRIMER_RIGHT_3_SEQUENCE=GCATGATTGTTACGTCTGCACT
PRIMER_LEFT_3=491,22
PRIMER_RIGHT_3=781,22
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=60.205
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.3728
PRIMER_LEFT_4_PENALTY=0.010077
PRIMER_RIGHT_4_PENALTY=0.362762
PRIMER_LEFT_4_SEQUENCE=TCGCCGTAATTGTTATCTCCTT
PRIMER_RIGHT_4_SEQUENCE=TCTTGGCATGATTGTTACGTCT
PRIMER_LEFT_4=496,22
PRIMER_RIGHT_4=786,22
PRIMER_LEFT_4_TM=59.990
PRIMER_RIGHT_4_TM=59.637
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_04490 Ps_contig_mira-and-tgicl-ass_franssen_33405
SEQUENCE=TTTTGTCTTAAATTCTAACTTCAATTTGTAATNAATTTCAAAGGCAAAATCAATTCTAATTGAAAACACTGAACATGTGTGACAATTTCTAGAATCTATTTTGAGGCACTAAACATACACATAGACACTCATACTGCACCAAGAAACTATAAAATCAACCAACAAACAATAAAAAGATTATAAAATTCAACTACTTTGTATTAATTCTTAACATTATGCTGTTAATGGTACAAAATGACACCTTCAAGTTCAAACAAAGCATGACATTTGCTCATAATACTCTTGGAGGTCATACCGTAACTTGACTTAGATTCATCATTACTCTTTCGAAGTTATCACGAGCAACCCCAAAGCACCAATGAGAATATACTTGATGATAGGTTTTTCCGTCATGATGATTGTCACCCAGCCAACATAAGGTAAGAACCCGACAGCTCTACCCATAATGTGGTGCCTTTGCAGCCACAGCTGACCATGAGCATACAGAAGCCTGTCATCGCCGTAATTGTTATCTCCTTTTGTGAGGACATCAACTTCCCCATTATCTCCCCTTTCGTGTACCTTAATTACACGATGAACGATCGGAATCTCTCGTCCATCAACATTAAATACAACAATCTCCCCTGCGCGAATTGGATCCTTACTCATGTGTAAGAACAAGATGTCCCCCCGCTTGAATCCCGGTTCCATACTTCCAGAAAGAACAACAACAACAGGAGATTCACTGCCGGTGATGCACATCAATGCCTTCCATATTATCAGTGCAGACGTAACAATCATGCCAAGACTAACACCCTGAGTGAGGACCTGACGGATCTGAAGAGATTTGATAGAATCCACAGTCTCACCGATCAAACCCATAGCTTCTTTCTTCTTTCTTTCTACCAAACTTCACCACTTTCTTGAGTTAACAAATGGAGAAAGAAACACAAAGACCAAACCAAGGCCAATTTCTACCTCCCCGAC
TARGET=872,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3386
PRIMER_LEFT_PENALTY=0.108206
PRIMER_RIGHT_PENALTY=0.230415
PRIMER_LEFT_SEQUENCE=ATCATGCCAAGACTAACACCCT
PRIMER_RIGHT_SEQUENCE=AGGTAGAAATTGGCCTTGGTTT
PRIMER_LEFT=775,22
PRIMER_RIGHT=958,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=60.230
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.3956
PRIMER_LEFT_1_PENALTY=0.108206
PRIMER_RIGHT_1_PENALTY=0.287386
PRIMER_LEFT_1_SEQUENCE=ATCATGCCAAGACTAACACCCT
PRIMER_RIGHT_1_SEQUENCE=CCTTGGTTTGGTCTTTGTGTTT
PRIMER_LEFT_1=775,22
PRIMER_RIGHT_1=945,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.287
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=171
PRIMER_PAIR_PENALTY_2=0.4170
PRIMER_LEFT_2_PENALTY=0.186600
PRIMER_RIGHT_2_PENALTY=0.230415
PRIMER_LEFT_2_SEQUENCE=TGACGGATCTGAAGAGATTTGA
PRIMER_RIGHT_2_SEQUENCE=AGGTAGAAATTGGCCTTGGTTT
PRIMER_LEFT_2=808,22
PRIMER_RIGHT_2=958,22
PRIMER_LEFT_2_TM=59.813
PRIMER_RIGHT_2_TM=60.230
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=151
PRIMER_PAIR_PENALTY_3=0.4353
PRIMER_LEFT_3_PENALTY=0.204906
PRIMER_RIGHT_3_PENALTY=0.230415
PRIMER_LEFT_3_SEQUENCE=AGTGCAGACGTAACAATCATGC
PRIMER_RIGHT_3_SEQUENCE=AGGTAGAAATTGGCCTTGGTTT
PRIMER_LEFT_3=760,22
PRIMER_RIGHT_3=958,22
PRIMER_LEFT_3_TM=60.205
PRIMER_RIGHT_3_TM=60.230
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=199
PRIMER_PAIR_PENALTY_4=0.4740
PRIMER_LEFT_4_PENALTY=0.186600
PRIMER_RIGHT_4_PENALTY=0.287386
PRIMER_LEFT_4_SEQUENCE=TGACGGATCTGAAGAGATTTGA
PRIMER_RIGHT_4_SEQUENCE=CCTTGGTTTGGTCTTTGTGTTT
PRIMER_LEFT_4=808,22
PRIMER_RIGHT_4=945,22
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=60.287
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_04491 Ps_contig_mira-and-tgicl-ass_franssen_33406
SEQUENCE=TGCAAGAGCAGACAGATATTGTTTGCCAGAAGGTGAAGGAAATGAAAGAATTGAAAGGAGGATACAACATTGTGGGACTCTCCCAGGGAAATTTGATCGGTCGAGGTGTGGTCGAGTTTTGTGAAGGAGGACCTCCCGTTAAGAACTTTATCTCTTTAGCAGGCCCTCATGCTGGTACTGCTTCAGTTCCCCTGTGTGGTACAGGTGTCTTTTGTGTCATTGCAGACAATCTCATCAAGGGAGAGGTTTATAGTAGCTATATTCAAGAACACTTGGCTCCTAGTGGATATCTTAAATTACCAAATGCTTTACCCAAATACTTGGAGAACTGTAGGTACCTCCCTAAGCTTAATAATGAAAAACCTGGTCAGAGGAATTCCACTTACAAGGAAAGATTTAGTAGCTTAAAGAATTTGGTACTTATCATGTTTGAGCAAGATACAGTTTTAATACCTCGAGAAACATCCTGGTTTGGTTATTATCCAGATGGCTCCTTCAAGCCAACATTACCACCGCAAAAGACAAAACTCTACACTGAAGATTGGATTGGATTGAGAACTTTAGACAATGCTGGCCGCGTCCATTTCATTAGTGTTCAGGGAAAACATCTGGGAATATCAGATGCAGATATGAAGAAACATGTAGTGCCATATTTGAACGAAACATCAAGAGAAGATAGAGCTACTGAGGCTGGCTTTCGAAGGATGCGTGAAGTTAACCGAAGATTTATTCAATTTAACAGGATGGTGTAATATCATACAGTGGAAGAAGAATCATCAAGCTCCAGCCACAACATTACTACTATGGATTGATTATTGTCATTTAGGTAGTGTATACCACGCAAATTGAGATTATTAACTTTTTTTCCTTCAAATTTATTGAATAACTGATATATATAATATCTATATAGATTTAATATATCGATTATTTAATGAATATGAAAAGAAAATCTTTATTAAGAATAGNAA
TARGET=540,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0055
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.003166
PRIMER_LEFT_SEQUENCE=TACCTCGAGAAACATCCTGGTT
PRIMER_RIGHT_SEQUENCE=TGAACACTAATGAAATGGACGC
PRIMER_LEFT=450,22
PRIMER_RIGHT=597,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.003
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=0.0267
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.003166
PRIMER_LEFT_1_SEQUENCE=ATGAAAAACCTGGTCAGAGGAA
PRIMER_RIGHT_1_SEQUENCE=TGAACACTAATGAAATGGACGC
PRIMER_LEFT_1=354,22
PRIMER_RIGHT_1=597,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.0267
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.003166
PRIMER_LEFT_2_SEQUENCE=AATGAAAAACCTGGTCAGAGGA
PRIMER_RIGHT_2_SEQUENCE=TGAACACTAATGAAATGGACGC
PRIMER_LEFT_2=353,22
PRIMER_RIGHT_2=597,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.003
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.0267
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.003166
PRIMER_LEFT_3_SEQUENCE=TGAAAAACCTGGTCAGAGGAAT
PRIMER_RIGHT_3_SEQUENCE=TGAACACTAATGAAATGGACGC
PRIMER_LEFT_3=355,22
PRIMER_RIGHT_3=597,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=60.003
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.0300
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.027715
PRIMER_LEFT_4_SEQUENCE=TACCTCGAGAAACATCCTGGTT
PRIMER_RIGHT_4_SEQUENCE=ATCTTCGGTTAACTTCACGCAT
PRIMER_LEFT_4=450,22
PRIMER_RIGHT_4=725,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=10.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_04492 Ps_contig_mira-and-tgicl-ass_franssen_33407
SEQUENCE=ACAACACCTRTGTTATGTGTTTTCTTCTGCCGTATCGTATNGGCATTCTACATTCCAAACAAACATCCCTTCCTTCCTTAACATTCCATAATCTTTTATTGGTCGGTTAAAGCTTCTTCCTTTCTCCCATCCTATCTTTCTTTTTCATTCTCCCAAAAATGGGTCTCTGGGATTCTCTTCTCAATTGGCTCCGCAGTTTGTTTTTTAAACAAGAGATGGAGCTTTCTCTTGTAGGTCTTCAGAATGCTGGCAAGACTTCTCTTGTCAATTCAATTGCTACAGGAGGATACAGTGAGGACATGATTCCAACTGTTGGGTTCAACATGCGAAAAGTGACCAAGGGGAATGTCACGATAAAGCTTTGGGATCTTGGTGGACAAAGGAGGTTTCGAACAATGTGGGAGCGTTACTGTCGCGGTGTCTCTGCTATTGTGTATGTTGTAGATGCTGCTGATAGAGACAGTGTTCCAATAACTCGAAGCGAATTAACTGAACTCCTGACAAAACCTTCCTTAAACGGGATTCCTTTGCTTGTTCTTGGAAACAAAATTGACAAGTCAGAAGCTCTTTCCAAGCAAGCATTAGTCGATCAACTAGGACTCGAGTCACTTACAGACAGAGAGGTTTGTTGCTATATGATTTCATGCAAGGATTCTGTAAACATTGACGTGGTCATCGACTGGCTTATCAAACACTCGAAAACTGCGAAATGATTGTGTTGATGAAGCTCCTTCNTAGGGTAATGCATACGTAACCTTTTAAGTTTTTTAGGTTTTTGGGGTACTAAAGTTNTTGGTTGTTA
TARGET=68,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04493 Ps_contig_mira-and-tgicl-ass_franssen_33413
SEQUENCE=CACCCCACTCTTCCTTCGCCATATCTCCCAACATCTCCCTCGACGGAGACAATATAGGTGTAGGAAGCACCGCCGGGGTCACAGGACAAGGCGACACAAGCGATGCAATACCACCAGATTTCTTGGCACGAATAATAAACGAAGTGGTATTCCTCGGCGCAAAAGGAGCAAATCTAGCATTAAACTTCTTCTTAGGATAGAACTTACGAGCTTTCAAACGATTCTGAGACTGCAACTCCTGAAGAGTAGGTGGTTGGTAACCGACACTGTTGTTGGTCTGATTGGGTTTTTCCATTCTCCTAATATCTTTCCTCTTGTCACCGCTAACCTTCTTTCCCTTCCAGTTGTTACTATTATTATTACTTCGCCCCGGTTTAGCGAAATTAGGTTTCAAATTAGGGTTCTGAAATTTCATGTTAGGATCCAAAGGAAGCTGCTGAGACCAAACCTTGTATCCTCCACGGTTCATCATCGGAGGCAGATTCTGGTTCATCATTTGCTGATTCATTCCTTGAAACTGAGACTGAGACTGATTAACAATCTGAGGCGGATTCATCTTCATCATCTGAGACTGAGACTGCGACTGGTTAATTATCTGAGGCGRATTCATCTTCATCATCTGAGACTGGTTCATCTGGCTCAAATTCATCATCATCTTCGATTGATCGTTCATCGTAACCAAACAAACAAACAAACCCTAACTACAAACAAACTAACAATTTCTTGAAATTCCAACGAATAATCAACGAAAAACAAACAAAAAAAAGTTAATCTCCGATCAGAAATTCTTGATCGGCGGCAGCGGTTATTCCGATTCAAAACCCTAAATCGATGTTTAGTGAAATTAAGAATCGATTAAAATTTTCAAGAAATTNAAGGAAAGGGTGAAATCGTGATGAAGAATCGAGATTTCACGAAAGGGGAGATTGAGAAACCCCGATTTTG
TARGET=680,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04494 Ps_contig_mira-and-tgicl-ass_franssen_33421
SEQUENCE=CGCTCAAAATCTCTGCATATTGTTATTGCTAATATTAATGACCGCTTATTGAACAGGCACAATAATATATTTAAGTTCTTGAAAAAAAGTAAAGCTAGGGGTCGATATGTAATTAGACATTTTCTAAGCATAGGTGCACACTATACAATATGCAATTTTGTTAATAAGAAAACTGTCATCTTCAACTGCTCTCCAATCTTGGTTCAGATGCACCTTCTTCATTTGAAGATGATTGTTCTTCAGCTCGTGCATTAGTCTGCACTGGTTTTTTGAACTGCACCAAAATCATTGTCATGTTATCGCATCCGTCACCCATAGCCAAAGACGGCGCCAAACACCGGTCGAGGACTCTTTCACAAACCTCAGAAAGTTTGGTATCCAAGAGAAGTTCTTGACGTACAAAATCTACCAATTGCTGGCTTGACAGGCAATCCCATATGCCATCACAAGCTATCACTATAAATTCATCTTCGTCGTGAAGGTCAACAATGTTTATGTCTGGATTAGCGGTGACAGCTTGTTTTTCAGCCGAAAGAAATCTATTATTCTTAAAGTCCACATCACCTATAGCTCTTGCTAGATTTAAACTCCCATTAACTCGTCCAGCGTGAATAAAACCTCCAGCTTTATAGATTCTTTCCTTCTCAATCGCGAGTTCCGGTTTGTGGTCTCTAGACAAATTGTAAGCCTGACCATTCCTAGATATTATGCAGCGAGAATCACCGGCATTTGCGACAAAAATTAGGTTGTTTCTAATGATTGCGACACAGGCAGTACTTCCTGAAGTTGGTCCATCAAAATCAGAATGAGGTCCCTTCTCAAATCCCCAATCATCACTTTGGTCCTTATTATTATTATTATCACTGCTTCTTGGAGACCGAATCAATCCTTCAATCATTCCATTGAATCCTTTTACCTTATCACCCAAGACCGCTAATTCCCTCCATCCCCTTTGACCACGCATCATCTCGTCCATTCTGAAAAATGCTTTCTGAAGTGATGTTCCAACATCTCCGGCTATGTATTCTTCACTCTTGAGTACCTGTTGGTGAAGATGCTTGGCGCAAAACTTTGCAACTGCCTTACCTCCATGACCATCATAAACTCCAAAAAATGAAGTGGATGAATCCACATCAAGATGTGCCGCATGTGCATCTTCCATTGTTGCACGCCAACCTTGCATAGATGATAATCCATATCTAAGATTCTCATTTTCGCCATCTTCAGAGAACTTTTCAGTTCTGRGAATGCTGAGATTTGTTCCCTATTCCTGTATCTGAATAAAACCAAATAAACATTAGTGTTATTTCTTTAAGCATGAAAATTAATCAAAACATACACAACAAAAATGTAATAAGAAAACCAAACTTAAA
TARGET=846,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04495 Ps_contig_mira-and-tgicl-ass_franssen_33423
SEQUENCE=GGTCTAAGTTCTTCTAATTTATTTACGTATGGTAGCAATTTATGTTAATGCATCAATTCTCAAAAAAGAAGCATATGTTAAATAAAGCTCAAATTGTATGACAAAAGAGCTATATATTCCCTTAAAATTTGCTATTATTATTCAAGAATCCTGAACCCAAATCCTCACAGTTCTATCACCATCAAGACCAGCAGAAGCAATTTTGTTCTCTCTTGGGTGGCAGGAAACAGCTATTACAGTATCTGTATGGCCTTCGAGTTTCTGAATCAAGTTTCTCAGCTGAAGATCCCAGAGATAAACACATCGATCTTCTGAACCGCTGACAATGTATCTCCCATTTGTCACAGAAAATGTGGAAGTTACGCAGTACACTTTATTCTCGTGGCCAGAATATATTTTCAAAAACTTCCCAGTAGCATAATTCCATAGCTTAAGGGTATTATTGAGAGTGGCAACGAGTATGAATTTTCCATTAGGTGAGAATCTGGCGAACGAAACAGCTGGAAGCTTATCATCGATCAGAGTCTTCAAGAGAGCACCGGCGTTTGTATCCCAAATCTTACAAGACCCGTCGTGACTACCGGAAACGATAAGAGAACCATCGCGATTGAAGTGAACGGAAGTAACAGGCATATCGTGAGCTTTGATAACATGGATACACTTCCCGGTCTTCACTTCCCAAACCCTAACGGTTTCATCGAAGGAGCCAGAGACGATGTAATTGGACTGAGGATTGAAATTGACGCAGAAAACGATGTGTGTGTGGCCACGGAGGATTTTGACACAGTCACCTCCGTTGGCGTCCCAGATGCGGATGGTACGGTCGTCGGAAGCGGAGCAGACGTAGTGGGAATCGGATGACCAGGCGACGTCGGAGATTCCTTCGGAGTGGCCGGTTAGGCGGTGGAGGAGAGAGAGTGTGGCGGAGGACCAAATGATTAGGGTTTTATCGAGGGAGCCGGAGGCGAGAAGTGTTCCATCGTTGGAGAATTTGACGCAGGAAACTGCGAGATTGTGCGCCGTTAGGGTTTTGAGGTGACGGTATGGTTTGTATGGCTGGCTGCTGTTGCTGCTATTGGTACTCGTCGCCATCGATTCCTTCGGCCTCTCTCTCCTCTTTCTTTCGTCTCTGTGTTTTCAATTTGATGTTAGTTTCTACCGAGATTCAGAAGCCCAACCAGGACTTGCTTTCCACTTTGAATCATCCCCTTCATTTGTCTTTCTTTCCCCTCCTTACTCTCTATATTATATTTCTCTCTTAATTTTCATACCCCGA
TARGET=756,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6605
PRIMER_LEFT_PENALTY=0.500551
PRIMER_RIGHT_PENALTY=0.159965
PRIMER_LEFT_SEQUENCE=TCTTCACTTCCCAAACCCTAAC
PRIMER_RIGHT_SEQUENCE=GCTCCCTCGATAAAACCCTAAT
PRIMER_LEFT=668,22
PRIMER_RIGHT=958,22
PRIMER_LEFT_TM=59.499
PRIMER_RIGHT_TM=59.840
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.6605
PRIMER_LEFT_1_PENALTY=0.500551
PRIMER_RIGHT_1_PENALTY=0.159965
PRIMER_LEFT_1_SEQUENCE=GTCTTCACTTCCCAAACCCTAA
PRIMER_RIGHT_1_SEQUENCE=GCTCCCTCGATAAAACCCTAAT
PRIMER_LEFT_1=667,22
PRIMER_RIGHT_1=958,22
PRIMER_LEFT_1_TM=59.499
PRIMER_RIGHT_1_TM=59.840
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.6981
PRIMER_LEFT_2_PENALTY=0.500551
PRIMER_RIGHT_2_PENALTY=0.197549
PRIMER_LEFT_2_SEQUENCE=TCTTCACTTCCCAAACCCTAAC
PRIMER_RIGHT_2_SEQUENCE=TCCCTCGATAAAACCCTAATCA
PRIMER_LEFT_2=668,22
PRIMER_RIGHT_2=956,22
PRIMER_LEFT_2_TM=59.499
PRIMER_RIGHT_2_TM=59.802
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.6981
PRIMER_LEFT_3_PENALTY=0.500551
PRIMER_RIGHT_3_PENALTY=0.197549
PRIMER_LEFT_3_SEQUENCE=GTCTTCACTTCCCAAACCCTAA
PRIMER_RIGHT_3_SEQUENCE=TCCCTCGATAAAACCCTAATCA
PRIMER_LEFT_3=667,22
PRIMER_RIGHT_3=956,22
PRIMER_LEFT_3_TM=59.499
PRIMER_RIGHT_3_TM=59.802
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.8205
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.786385
PRIMER_LEFT_4_SEQUENCE=CGGAAACGATAAGAGAACCATC
PRIMER_RIGHT_4_SEQUENCE=GGTGACTGTGTCAAAATCCTCC
PRIMER_LEFT_4=581,22
PRIMER_RIGHT_4=791,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=60.786
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_04496 Ps_contig_mira-and-tgicl-ass_franssen_33428
SEQUENCE=TGAGGACGAAACAAACTTTTACTTTGGGTAGGATTAGCATTCTTACCTCTCTGCCACTCCTATGGATGAAGAAGTGGTAGCTAATGCCTCTGTGGTGAAGAGTGGAAGAAATTTGACTGTAGTTGCACTTGAATTCAAATTGAAGAAAACTGGGAATTTGCTTTATCTTAGTCATGCTACCTTCTTTAACATGCCCGTTTCCAGCTTATGATCACTTATCCTTCTGAGGTTTTCTTTTCTTTTCAGTTTGGGGAAAGTGNTTATTAATC
TARGET=230,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04497 Ps_contig_mira-and-tgicl-ass_franssen_33435
SEQUENCE=CCTTTCTTTTTCTTGTGCGCGAATTGAAAGAAATTTGTAAGTAGTAGTAGTTGGTTACCTACCGGAATCGATTTGTTGACGGTGCGGTTGGTTGTYCGTTYTGTGAGTTCCTGCAGCATCGGCTAAACCGAGAGCGAAGAGGGAGAGCGTACTCGTGGCGCAATGGAGCTTCGAAGATGGAAAGCAGAATCGAAGCTGTGGATTCACCGGTTCAGAACTTGGAGCTTAGAAGTAGCTTGGCTTGTATTGTAGCAGCTTACGCTTGGCTCATTTGGAAGTTTGGTATGCGTTTCAACTTGGCGTTGATTGTGCGTTTGGAGCTGGCTTATGCGTTTTCCGGTTTGGCTTATGGCGTGTGGCTCGTAGCTTGTGGGAGCTTGGAAGTAGTCTTTTGTTTCACATTATTTTTCCTGCACTGCCGTCTATTTTCTGCTACTGTCCTGCGTCTTTGAAGTCTTTCTTATTTTCCTTTTTGTTATTTTAGTTTTGGGTGTACGTTTGGTCTTTGTTCTTATTT
TARGET=40,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04498 Ps_contig_mira-and-tgicl-ass_franssen_33438
SEQUENCE=ATTGTTATAAAAATATACATATACATTACATATAGACTCACATTCATACCACTCTTCACTTCCCTCTTTGCTTAGTTAGTTGCTTCCATTATTTTTCAACTAATTCTCCAAAATGAAGAAGCAACTAGCACCACTTGTTCTATTCTCTCTCACACTAATACTATCACTTATTCACTCCACCACCGCCGGTGCCGCCACCTCCGGCAGCCACAACATCACCCGCATTCTCGGCAAACACCCTGGATTCTCCACCTTCAACCACTACCTCACCATCACTCACCTCGCCGACGAAATCAACCGCCGCCAAACCATAACAATCCTCGCCATCGATAATGCCGCCATGTCGTCCCTTCTCGAGAAACACCTCTCCGTCACCACTCTCAAAAACGTCCTCTCCCTTCACGTCTTAGTCGACTACTTCGGCGCGAAGAAACTCCACCAAATCACGAACGGTACAACTCTAGTTTCGTCCATGTTTCAAGCAACCGGAGCCGCCGCGGGAACTGCCGGTTACGTCAACGTCACGAATCTCAAAGGCGGCAAAGTCGGTTTCGGCGCGGAAGACAACGACGGCCTACATTCTTTCTACGTGAAATCCGTTGAAGAACTTCCTTACGTCATCTCCGTACTTCAAATCAGTCAACCTCTAAGCTCCGCCGACGCCGAAGCACCGACGGCATCTCCGAGCGATATCGACTTAATCTCAATCATGTCAAAACAAGGTTGCAAAGCCTTCGCCGATTTGCTACGTGTTTCAAAAGCTCTTCCGACTTTCAAAGACACCGTTGACGGCGGTTTAACCGTTTTCTGCCCAACTGACAGCGCCGTTAACGGTTTCGCTGCTAAATACAAAAACCTAACCGACGCCAAGAAAGTTTCACTTCTTTTATATCACGGAGTTCCCGTTTACCAATCGTTACAGATGCTGAAATCTAACAACGGTATCATGAACACGCTAGCAACAGAAGGTGCTAACAAATATGATTTCACCGTCCAAAACGACGGTGAAGACGTGAACCTTGAAACCAAGGTTAACACCGCCAACATTATCGGAACACTGATCGATCAAGATCCGTTTGTGGTGTATAAGATCAACAAGGTTTTGATGCCGAGAGAGTTGTTTAAGGGAGTTAAAGAAGAATTAGCACCGGCAGAGTCACCGAAGGCGGCGAAGAAGAGCAAAGAAAGAAAGGGTAAGGCGGGCGGGCGGATGAAAGATGGCGGATTCCTCCGAGGCGGGCGGTAGTGGGACCGGATGGCGG
TARGET=1181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9140
PRIMER_LEFT_PENALTY=0.117281
PRIMER_RIGHT_PENALTY=2.796703
PRIMER_LEFT_SEQUENCE=TTGATGCCGAGAGAGTTGTTTA
PRIMER_RIGHT_SEQUENCE=AGGAATCCGCCATCTTTCAT
PRIMER_LEFT=1102,22
PRIMER_RIGHT=1229,20
PRIMER_LEFT_TM=59.883
PRIMER_RIGHT_TM=60.797
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=2.9559
PRIMER_LEFT_1_PENALTY=0.159203
PRIMER_RIGHT_1_PENALTY=2.796703
PRIMER_LEFT_1_SEQUENCE=GGTATCATGAACACGCTAGCAA
PRIMER_RIGHT_1_SEQUENCE=AGGAATCCGCCATCTTTCAT
PRIMER_LEFT_1=940,22
PRIMER_RIGHT_1=1229,20
PRIMER_LEFT_1_TM=60.159
PRIMER_RIGHT_1_TM=60.797
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=3.0133
PRIMER_LEFT_2_PENALTY=0.216588
PRIMER_RIGHT_2_PENALTY=2.796703
PRIMER_LEFT_2_SEQUENCE=ATGCCGAGAGAGTTGTTTAAGG
PRIMER_RIGHT_2_SEQUENCE=AGGAATCCGCCATCTTTCAT
PRIMER_LEFT_2=1105,22
PRIMER_RIGHT_2=1229,20
PRIMER_LEFT_2_TM=59.783
PRIMER_RIGHT_2_TM=60.797
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=3.0190
PRIMER_LEFT_3_PENALTY=0.222249
PRIMER_RIGHT_3_PENALTY=2.796703
PRIMER_LEFT_3_SEQUENCE=CCTTGAAACCAAGGTTAACACC
PRIMER_RIGHT_3_SEQUENCE=AGGAATCCGCCATCTTTCAT
PRIMER_LEFT_3=1017,22
PRIMER_RIGHT_3=1229,20
PRIMER_LEFT_3_TM=59.778
PRIMER_RIGHT_3_TM=60.797
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=3.0288
PRIMER_LEFT_4_PENALTY=0.232059
PRIMER_RIGHT_4_PENALTY=2.796703
PRIMER_LEFT_4_SEQUENCE=TTAAAGAAGAATTAGCACCGGC
PRIMER_RIGHT_4_SEQUENCE=AGGAATCCGCCATCTTTCAT
PRIMER_LEFT_4=1130,22
PRIMER_RIGHT_4=1229,20
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=60.797
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=12.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=100
=
PRIMER_SEQUENCE_ID=SSR_04499 Ps_contig_mira-and-tgicl-ass_franssen_33438
SEQUENCE=ATTGTTATAAAAATATACATATACATTACATATAGACTCACATTCATACCACTCTTCACTTCCCTCTTTGCTTAGTTAGTTGCTTCCATTATTTTTCAACTAATTCTCCAAAATGAAGAAGCAACTAGCACCACTTGTTCTATTCTCTCTCACACTAATACTATCACTTATTCACTCCACCACCGCCGGTGCCGCCACCTCCGGCAGCCACAACATCACCCGCATTCTCGGCAAACACCCTGGATTCTCCACCTTCAACCACTACCTCACCATCACTCACCTCGCCGACGAAATCAACCGCCGCCAAACCATAACAATCCTCGCCATCGATAATGCCGCCATGTCGTCCCTTCTCGAGAAACACCTCTCCGTCACCACTCTCAAAAACGTCCTCTCCCTTCACGTCTTAGTCGACTACTTCGGCGCGAAGAAACTCCACCAAATCACGAACGGTACAACTCTAGTTTCGTCCATGTTTCAAGCAACCGGAGCCGCCGCGGGAACTGCCGGTTACGTCAACGTCACGAATCTCAAAGGCGGCAAAGTCGGTTTCGGCGCGGAAGACAACGACGGCCTACATTCTTTCTACGTGAAATCCGTTGAAGAACTTCCTTACGTCATCTCCGTACTTCAAATCAGTCAACCTCTAAGCTCCGCCGACGCCGAAGCACCGACGGCATCTCCGAGCGATATCGACTTAATCTCAATCATGTCAAAACAAGGTTGCAAAGCCTTCGCCGATTTGCTACGTGTTTCAAAAGCTCTTCCGACTTTCAAAGACACCGTTGACGGCGGTTTAACCGTTTTCTGCCCAACTGACAGCGCCGTTAACGGTTTCGCTGCTAAATACAAAAACCTAACCGACGCCAAGAAAGTTTCACTTCTTTTATATCACGGAGTTCCCGTTTACCAATCGTTACAGATGCTGAAATCTAACAACGGTATCATGAACACGCTAGCAACAGAAGGTGCTAACAAATATGATTTCACCGTCCAAAACGACGGTGAAGACGTGAACCTTGAAACCAAGGTTAACACCGCCAACATTATCGGAACACTGATCGATCAAGATCCGTTTGTGGTGTATAAGATCAACAAGGTTTTGATGCCGAGAGAGTTGTTTAAGGGAGTTAAAGAAGAATTAGCACCGGCAGAGTCACCGAAGGCGGCGAAGAAGAGCAAAGAAAGAAAGGGTAAGGCGGGCGGGCGGATGAAAGATGGCGGATTCCTCCGAGGCGGGCGGTAGTGGGACCGGATGGCGG
TARGET=1198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9140
PRIMER_LEFT_PENALTY=0.117281
PRIMER_RIGHT_PENALTY=2.796703
PRIMER_LEFT_SEQUENCE=TTGATGCCGAGAGAGTTGTTTA
PRIMER_RIGHT_SEQUENCE=AGGAATCCGCCATCTTTCAT
PRIMER_LEFT=1102,22
PRIMER_RIGHT=1229,20
PRIMER_LEFT_TM=59.883
PRIMER_RIGHT_TM=60.797
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=2.9559
PRIMER_LEFT_1_PENALTY=0.159203
PRIMER_RIGHT_1_PENALTY=2.796703
PRIMER_LEFT_1_SEQUENCE=GGTATCATGAACACGCTAGCAA
PRIMER_RIGHT_1_SEQUENCE=AGGAATCCGCCATCTTTCAT
PRIMER_LEFT_1=940,22
PRIMER_RIGHT_1=1229,20
PRIMER_LEFT_1_TM=60.159
PRIMER_RIGHT_1_TM=60.797
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=3.0133
PRIMER_LEFT_2_PENALTY=0.216588
PRIMER_RIGHT_2_PENALTY=2.796703
PRIMER_LEFT_2_SEQUENCE=ATGCCGAGAGAGTTGTTTAAGG
PRIMER_RIGHT_2_SEQUENCE=AGGAATCCGCCATCTTTCAT
PRIMER_LEFT_2=1105,22
PRIMER_RIGHT_2=1229,20
PRIMER_LEFT_2_TM=59.783
PRIMER_RIGHT_2_TM=60.797
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=3.0190
PRIMER_LEFT_3_PENALTY=0.222249
PRIMER_RIGHT_3_PENALTY=2.796703
PRIMER_LEFT_3_SEQUENCE=CCTTGAAACCAAGGTTAACACC
PRIMER_RIGHT_3_SEQUENCE=AGGAATCCGCCATCTTTCAT
PRIMER_LEFT_3=1017,22
PRIMER_RIGHT_3=1229,20
PRIMER_LEFT_3_TM=59.778
PRIMER_RIGHT_3_TM=60.797
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=3.0288
PRIMER_LEFT_4_PENALTY=0.232059
PRIMER_RIGHT_4_PENALTY=2.796703
PRIMER_LEFT_4_SEQUENCE=TTAAAGAAGAATTAGCACCGGC
PRIMER_RIGHT_4_SEQUENCE=AGGAATCCGCCATCTTTCAT
PRIMER_LEFT_4=1130,22
PRIMER_RIGHT_4=1229,20
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=60.797
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=12.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=100
=
PRIMER_SEQUENCE_ID=SSR_04500 Ps_contig_mira-and-tgicl-ass_franssen_33440
SEQUENCE=TACGCGGGGCGTCTAACAACTACCTTACTCTCCANTCRRNNGGACACACACACTCACGCGCTCTCATCCATTCACTCATCTCTCAAACTCCTCACTCACTCCCTGATTTCATCCCCTCACCTTCGTTCTCACAAAAAAGGGGATTTCCTCTCACAAAACCATCGCTACACCCCGCCGCCATCATCTTCCTCACCCGGCCTCTATCTTCATCGTCCAAACACCACCATTCATAACCAAAACCACATGAAACCCTCTTCTTAAAACTCATCGTCTTCCAAACTGTTCATTAAATCACCACCGTTTTCTCCTTCATCATCAATCACCTTCAATGCCACCTAAACCCCAAGCCGCCGTTACAATCATCTTCAAACCATAAACCCTCTCCACAAATCCTTCATCTCCATCCGGAAACGCCGCTCCATCTCTCCCACCATGCAAAGCGACGAACCCCCAAACACACAACCCTGAAATTTCACCCACGAATCCACAGCTCCCACCATCAGAATTTTCGATTCAACCACACATCTCCACCTGCACAGAAAACCCAATCGGAACCTCTTCCGTCCATTAATGTGTCATCTTTGAAAAGAAATTAATGGTTTCAGTTTTAGCTTTACGTTGTCACACCGCCTAAGGATCGATGGTTTGCTCTTTGGACTGGTGAAAGAAGAAGGCAAGCGGTAGCCGGAATCCTCGGACCCTTCCTCAACAAAGACCTTACAATCTCCAGGCTTCCTAACTCGTTTCTGGTCTCGCCGTCTCTAAAGAAGCTCAGGGTTGCGGTTCGATGTGAGTAACAAGACGACACGTTCCGCACTTGGTAACCTCTTTGAACCAGGGTATTATTATAGACTTTAATTTTTTTTTTACTAAAAAAAACTCTCAACATATATTTTCATGTTTGTGATCTTGTTTTGGATATTGATATGGTTATTGCACATACCATGAGTCAATGACCGTGTTTATGTTTGTATTTGGGCGCCTGGTTCAATTTTAATTTTATGGCTTTGTGAACAAGTTTTGTTGTTGACTCTTGTTGTACACTGTTTCGTGAAGGAGGTATTAGGAAACTGGTATGTAAGGTTTTTGTTTTGTGCTGTATGTGATAGTCTGAAGTTGGTGGATTGATTGTTGTTTGTTTTTATGATATGAGTTAAGGATTGTTAAATTAAATCATGAGCATGGTGTAATATATATTCTTACGGTTTAAAACCTCTGCATTATTTTTGTAAAAAATTGT
TARGET=44,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04501 Ps_contig_mira-and-tgicl-ass_franssen_33452
SEQUENCE=AACCTTTAAAAATAATGTTGTTTCTTTGTAAAAATTAATTATTTTATTTTATTTGCCCGGCCTACTATTCCATCACTTTTTTCTCCGTTCATCATGTCCGAACCTTCCCAGTCCCAACCTTTTCCCAATCATGGCGGCGTAGCCTGATTCAAAACCCTAAACCCAACCCTTCACTTCCCCGTTCCTACCAAACTCCAATGGCTACTGCACTCGCTCTCAGATTCAGATCCAATCTAACCCGCACCGCCTCACTCCTCATCTCCTCTCTCCATCCACCACAATTCAAACCCTCGCCGCCACTCTTCTCTTCCTTCTCACTCACCCCTTCCACCCCCCTCGATTCCACCTCCTGGTTCTCCCTCTCAACCCACCGCTTCCTCTCCACCGCACGCCGCCAACCTACATCGCGGCCTAAGAAAACCATCGACATCGCCGCACGCGCTCGACAACTCCAAGCCAAGCGTCTGTGGACCTACGGACTCTCATTCGCGACGTTCGCCGGTTTCATCGTCGTCGTTCTAAACAACTTCCAGGATCAATTAGTCTTTTACGTCACTCCAACCGACGCAATAGATAAATTCCGAACTAACCCTACCAAAGCCAAGTTTCGATTAGGCGGTTTGGTTCTCGAAGGAAGCGTTGTGTATCCATCTTCTTCTCCAGGTGTGGAATTCGTGATCACCGATTTGATTACCGATATAGTTGTTCGCCATGAAGGCTCTTTGCCTGATTTGTTCAGGGAAGGACACTCTGTTGTTGCTGAAGGGTTTGTGAAGCCTTTCACAGATGAAATCAAAAAAGATAATTCTTTGAAGAAAGTTTCTGCGAAGGCTAGGAGTGGAGAGTATTATTTTTCTGCTACTGAAGTTCTTGCAAAGCATGATGAGAAATATATGCCTAAAGAGGTTGCTGCTTCAATTGAGAAGAATAAAAAGATTCTTGAGGAAGCTAATGCTGTGCCTGAAGTGAAACCCAACATCGCTTCATAATTTGATGGTATTGCTCAACTTTGAATTTTAATTTTATTGAGAAATATGTTACAGTGTTGTGATTTTTTAGGGGTTTGGATTGGATATTATTTAACTATACGGGAGAATTTTTTTATATAATAATTAGGAGG
TARGET=39,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04502 Ps_contig_mira-and-tgicl-ass_franssen_33466
SEQUENCE=TTGACACACTGGATGGTGGTACGATAGAACTGGCTTTGGACCCTCATTTAAACAAAACAGGGGACATTTGGCACATACGTATTGAGGATCTTNCTAGGAGCAATGTTCTGTACGGGTAGTCGGATAGATGGATCTCAAGACTGGGGTAAGGGGGCGATCGATTTGACAGAAGCATTGTTGTCTTGTTGATCCTTATGCAAAACTAGTTGAAGGTCGGAGATATTTTGGGGATATTAGCAAGAAGTTTTCTAAGTTTCTGGGCACATATGATTTTGACAGCTTGCCTTTTGACTGGGGAGAGAATTATAAGCTTCCAAATGTTGCCGAGAAAGATCTTGTTATATATGAGATGAATGTCCGTGCATTTACAATGGATGAATCTAGTGGGTTGGATAACAACATTCGTGGAAGCTATCTTGGCGTGATTGAGAAGATCCCACATCTTCTAGAGCTTGGCATCAATGCAGTGGAGTTGCTGCCTATCTTTGAGTTTGATGAGTTGGAATTGCAGAGGCGTCCAAATCCTAGAGATCACATGATTAATACGTGGGGTTATTCAACAATAAATTTCTTTGCTCCAATGAGTCGCTATGCTAGTGCTGGAGGAGGACCAGCTAATGCTTCCCAAGAGTTCAAGCAAATGGTTAAAGCCTTACATTCTGCTAGCATAGAGGTTATTTTGGATGTTGTCTACAATCATACTAATGAGGCTGATGACCCCAATCCTTACACTACTTCATTTCGTGGCATAGATAATAAGGTTTATTACATGCTGGACGACAAAGGACAATTGCTGAACTTTTCTGGCTGTGGAAATACATTGAATTGTAACCATCCTGTGGTCATGGAGCTAATTCTTGACAGCCTAAGACATTGGGTAACTGAGTATCATGTTGATGGATTTCGATTTGATCTTGCAAGTATATTGTGTCGAGGAACTGATGGTTCACCCCTCAATGCTCCTCCACTCATCAGGGCAATTGCTAAGGATGCAGTCTTGTCAAGATGTAAAATTATTGCAGAGCCTTGGGACTGTGGTGGTCTCTATCTTGTTGGAAGTTTTCCTAATTGGGATCGATGGGCTGAATGGAATGGGAAATATCGTGATGACGTACGGAGATTTATCAAGGGTGATTCTGGTACCAAGGGGAGCTTTGCAACTCGTGTTTCTGGATCTTCTGATCTTTACAGAGTGAACAAACGCAGACCATATCATGGTATTAATTTTGTTATTGCACATGATGGATTCAGCCTGCATGATCTTGTTTCATACAACTTGAAGCACAATGAAGCTAACGGGGAAGGTGGAAATGATGGATGCAATGATAACTTTAGTTGGAATTGTGGTTTTGAAGGTGAAACTGATGATACTAGTATAAGAGCTTTGCGGTCGAGGCAAATGAAAAACTTTCATTTGGCATTAATGGTTTCTCAGGGAATACCAATGATGCTAATGGGGGATGAATATGGTCATACTCGCTATGGAAATAACAATAGTTATGGGCATGATTCTGCCATTAATTTTCTCCTGTGGGATCAGTTAGATGCAAGGAAGGGTGATCACTTTAGGTTTTTCTCCAATGTGATAAAGTACCGGCTTGGACATAAAATATTCAGTCATGAAAATTTTCTCAGTGAGAATGAAATAACATGGCATGAAGACAATTGGGACAATTATGAAAGCAAATTTCTTGCATTTACGCTTCATGACAAAAGTGGGGGAGACGTCTATTTAGCTTTTAATGCTCATGACTACTTTTTGAAAGTTCTGCTTCCCACACCCCCAACAAAGAGAAGGTGGTATCGCGTGGTGGACACTAATCTTGAATCACCTGATGACTTGGTCCTTGACGGTGTTCCTGGCATAGGAAAAACGTACAGTATAGCTCCATACTCCTCCATTCTTCTGGAGGCAAAGTTTTGATTGCAGAAGCTGTTGTATACATGTATCAGTAAAGGAATAAAAAAGGAAAAAGGGAAAAAGACAGTGTATATAGCGTGTATCAGTGTAATGTTAATCTTATTTTCTTTTCAAGGAAACAAGATGAAATTTATGCTCTGAATTAGGTTCCCTGGCTCAGTATGGCATGAAGTTTTACAGCTCAGGATTAGAAGACGCCTGTATCAAATAGAAGATAAACGTTTCTGAATAGAAATGTGTTGTAGGAATAATCGTAGGAGAGGGAAATCTGAAATCATATCATTTTCAGAATGGTGCAGGGTATATATGCAAGGTACAAGCTCTACTTGAGAGTGGTCCTACGAAGTATGGATAGTCAATTACTACTACTACTACCGTGTCTACTATATATTTGTTTGATAAAATGTCTATATGTGATTTTATATTTACGCATGTATACTGAGATCCTATGTACTGTTGTTATATTGTAAATTTCACTTGAATGGAATAAATTCATTTAGTTTGAGAAGNNTACRTTCC
TARGET=2282,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04503 Ps_contig_mira-and-tgicl-ass_franssen_33471
SEQUENCE=GAATTTAAACCGATTCAAGAATATTTGAATGAGATATATCCAAACCGGTTTAAACGGCTTGTTCTATTTCGCTGTCAAATTTCACTTCCGTTANGTCAACGTAACTCTTCTTTTCTTCTCATAGCTTGTTTAACCTCCACGTGTTCTTGTTCCTTCTCTTCAGCCACCACCACTACTACTACTACCCCTCCTCTCATTTTCCTTCTCCCAATTAGTCTTTTCTTCTGCCCTAAATGGCCGAAGAAACAGACTCCAAACTCACCCAATTCAAAAACTCTGTCACAAATCTTGGTTCTTCAACACAGGGTTATGGGGACGCTACACTGATGAGGTTTTTGATTGCTCGCTCTATGGATTCTGATAAAGCAGCTAAGATGTTTGTTCAATGGCGTAAATGGAGAGAGACTATGGTTCCTAATGGGTTTATTTCAGATTCTGAAGTTCCCGATGAGTTGGAGACTAGAAAGATCTTTTTGCAGGGTTTGTCTCAAGATATGTTTCCTCTCATGATTGTTCAAGCATGTAGACACTTCCCATCCAAAGATCAGAATCAATTCAAGAAATTTATCGTACATTTACTGGACAAGACCATCGCGAGTGCTTTTAAAGGAAGGGAAGTAGGAAATGAAAAGTTGATTGGAGTTCTTGATCTGCAGAATATTTCTTATAAGAACGTTGATGCACGTGGTTTAATTACTGGATTTCAATTTTTACAGAGTTACTACCCTGAACGTTTAGCAAAGTGTTATATTTTACACATGCCATGGTTTTTTGTGAGTGTTTGGAGACTAGTTTCTGGCTTCCTAGACAAGGCAACACAAGAAAAGATTGTGATTATAAGCAATGAAGATGAGAGAAAGGAATTCATAAATGAAGTTGGTGAAGAGATTCTCCCAGAAGAGTATGGTGGAAGAGCAAAGCTTGTAGCCATCCAAGATTTTGAAGTGACACCATTAGAAAATGGAAGAACAAATTGATTAAAGTGTTTACTTGACAATCAAACAATCATTTTAGAGTACAATAAAATAAGGGGTATTTTATGCTTATAACTAGAGGACATTATACCAAACCAAATCATGATTAGAAATAAACCAACATTGTTTTTTTATCCATTTGATTTGGTTTGAATTTTTAGATCATAGATTGTAAAAGTGAGACTAAATCAACTATATTTTATTGGTTCATATTTTTATATAANNAATCCTAGCCAATCTGACAGTTACATTTGGTAG
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0186
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.016312
PRIMER_LEFT_SEQUENCE=TTCTTGTTCCTTCTCTTCAGCC
PRIMER_RIGHT_SEQUENCE=GGGTGAGTTTGGAGTCTGTTTC
PRIMER_LEFT=143,22
PRIMER_RIGHT=263,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.016
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.1017
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.099438
PRIMER_LEFT_1_SEQUENCE=TTCTTGTTCCTTCTCTTCAGCC
PRIMER_RIGHT_1_SEQUENCE=ATTGGGTGAGTTTGGAGTCTGT
PRIMER_LEFT_1=143,22
PRIMER_RIGHT_1=266,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.1314
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=TTCTTGTTCCTTCTCTTCAGCC
PRIMER_RIGHT_2_SEQUENCE=GTCTGTTTCTTCGGCCATTTAG
PRIMER_LEFT_2=143,22
PRIMER_RIGHT_2=250,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.1314
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.129103
PRIMER_LEFT_3_SEQUENCE=TTCTTGTTCCTTCTCTTCAGCC
PRIMER_RIGHT_3_SEQUENCE=AGTCTGTTTCTTCGGCCATTTA
PRIMER_LEFT_3=143,22
PRIMER_RIGHT_3=251,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=60.129
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=0.1550
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.152712
PRIMER_LEFT_4_SEQUENCE=TTCTTGTTCCTTCTCTTCAGCC
PRIMER_RIGHT_4_SEQUENCE=AGAATCCATAGAGCGAGCAATC
PRIMER_LEFT_4=143,22
PRIMER_RIGHT_4=358,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=59.847
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_04504 Ps_contig_mira-and-tgicl-ass_franssen_33473
SEQUENCE=AAGTTAGTGTTAGGTCGATCACTACTCAGNCCCGTTCCTAANGACTCGTAAAGAAGGCGAAATAGCTCTTAGCTGCTGCTCAAGGATTAGCTGACAAGGTTTCCTTTCGAGTTGCTGATGCTCTAGAGCAACCATTCCCTGACGGCCAGTTTGATCTGGTGTGGTCGATGGAGAGTGGAGAGCATATGCCTAACAAACCAAAGTTTGTTGGAGAGTTAGTTCGGGTAGCAGCACCAGGTGGCACCATAATTATAGTAACATGGTGCCATAGGGATCTTCGTCCCGACGAAAAATCCCTACAACCATGGGAGGAGAATCTCTTGAAGAAGATATGTGACTCATTTTATCTTCCAGCATGGTGCTCAACTGCTGAATATGTCAAATTGCTTGAGACCATGTCCCTTCAGGACATCAAATCAGCAGATTGGTCTCCCTTTGTTGCTCCATTTTGGCCAGCAGTTATACGTTCAGCGTTAACATGGAAGGGTTTCACTTCAATCTTAAGGAGTGGGCTAAAAACTATAAAAGGAGCTTTGGCTATGCCATTGATGATAGAAGGATTCAGGAAGGGTGTAATTAAGTTTGCCATTATCACATGTCGAAAGCCTGAAAACTAGGAGGGTCGATGATTTTATATTTTAGTATACCGAAACAGATTCTTACATGTCATTTTTCTTTTGGCAGTTCACAAATAGAAGAAATAAAGATAAGTGTTCTGCCACATCAAATCTTATTAGGATTGAATAACTGATATTGAAGGAAAAATGTAGCTTGCAATCGAAATGGTTGCTCAATATTGCTTTACAAACAAACAAAAATGTTTGTTTANTTAACACTCATTTATTG
TARGET=804,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04505 Ps_contig_mira-and-tgicl-ass_franssen_33482
SEQUENCE=TTACAGCACCACCCGAACCTGGATCGCGTGACGCTGCTGATATAAAACCAATTTGCTCCTTTCGTTCCCAAAAGTAACGGTAGTAACTACTCAGAGGGTGCTGTTACCAAAAAAATAATAGTACCAGTCACTAGCAGGCGTCATGCTGAACCTTCAACCGAGAAATATGCATCTTCGGGAGATGAATCTGATGATGATGATGAATCTGATGATGAATTTCATCATCAAACAATCCCCGTTCATACCTCGCCGCGTAAAGTTGTTGATAAGTTCCCGGCCAATGTGCAGCAGAATCATGAAGGTGGCCGTATTCCTCAAAGATTGGGTCCTATTGTTGATCGCGGTCGCCATAGTCCACAGCCAACCGCTGAAGGACGCAAACCAGTTGGAATTAGTCCAATTAAGAATGATAAATACCATGGCAGTGGTGACCATCCTGCAGCT
TARGET=189,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1276
PRIMER_LEFT_PENALTY=0.061230
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=GAGAAATATGCATCTTCGGGAG
PRIMER_RIGHT_SEQUENCE=ACTAATTCCAACTGGTTTGCGT
PRIMER_LEFT=159,22
PRIMER_RIGHT=395,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.1838
PRIMER_LEFT_1_PENALTY=0.061230
PRIMER_RIGHT_1_PENALTY=0.122569
PRIMER_LEFT_1_SEQUENCE=GAGAAATATGCATCTTCGGGAG
PRIMER_RIGHT_1_SEQUENCE=GGACTAATTCCAACTGGTTTGC
PRIMER_LEFT_1=159,22
PRIMER_RIGHT_1=397,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=59.877
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.2259
PRIMER_LEFT_2_PENALTY=0.061230
PRIMER_RIGHT_2_PENALTY=0.164639
PRIMER_LEFT_2_SEQUENCE=GAGAAATATGCATCTTCGGGAG
PRIMER_RIGHT_2_SEQUENCE=GACCCAATCTTTGAGGAATACG
PRIMER_LEFT_2=159,22
PRIMER_RIGHT_2=327,22
PRIMER_LEFT_2_TM=60.061
PRIMER_RIGHT_2_TM=59.835
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.2318
PRIMER_LEFT_3_PENALTY=0.061230
PRIMER_RIGHT_3_PENALTY=0.170538
PRIMER_LEFT_3_SEQUENCE=GAGAAATATGCATCTTCGGGAG
PRIMER_RIGHT_3_SEQUENCE=CAATAGGACCCAATCTTTGAGG
PRIMER_LEFT_3=159,22
PRIMER_RIGHT_3=333,22
PRIMER_LEFT_3_TM=60.061
PRIMER_RIGHT_3_TM=59.829
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.3089
PRIMER_LEFT_4_PENALTY=0.061230
PRIMER_RIGHT_4_PENALTY=0.247631
PRIMER_LEFT_4_SEQUENCE=GAGAAATATGCATCTTCGGGAG
PRIMER_RIGHT_4_SEQUENCE=GTCACCACTGCCATGGTATTTA
PRIMER_LEFT_4=159,22
PRIMER_RIGHT_4=431,22
PRIMER_LEFT_4_TM=60.061
PRIMER_RIGHT_4_TM=59.752
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_04506 Ps_contig_mira-and-tgicl-ass_franssen_33485
SEQUENCE=CTGAGCATTGTATTCAAAAACTGTCACAAAAATTACCAACAAATTCAGTTCTTTCTTTCTTTTTTACCATAATATAAAATTTCCAGCATAACTGAAACTGCAATCACAATTTAAAACAAATATTTAGAGTTAGGGGACTTACCGATAGTGTCACCAATTTGGAAGTTCTTAGTAGCAGACCATTCTTGTAATCTACGCTACCAATGATTGTCCAACCAGAAGAGTCTCCAACCTTGTGCACAGCTGCATTTGAAACCTGAACAGCCACCATTGTCATCATCAAGAAAACTAATACATCTCTCTACCAATTCCATGGTTTTCTAACTCTGCAAAGTAATCAGAATTTTTTCCCTGAAGTGTGTGTTCATCGAGTA
TARGET=49,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04507 Ps_contig_mira-and-tgicl-ass_franssen_33491
SEQUENCE=CCCGATCGGAANGGGCCGAGTGAGTGAAGTTTAGGGCACTTCTTCATCTTCAGAGCARGCTCGCCGCCGCTATCAGCTTTCTCTCCGGCGACACTCATTTTCACAATCTCAACGGTAAGAGTCTATGAATGAATAAACTATAGCTTATTTCTTCAATCAAATCAAAAATCGATTATTTCACCATGCTTTCTCGTTTAATCCCCAAAAAACCCTCTCATTTCCGCCGCCTTTTCTCCCTCCCTTCCAAACAACCCTATTTCCCCAATTCACCATTCAATCCGCACTTCACCGCTCTTCCAAAACCCTTCTCCAGCAGCAGTAACAATAACAACAACAACACCAAGGATCCGGTATGGAAGAGTTTCGGAGAATCCAAGGAGAAGTTCGACAATTTGTTTACGGAAGAAAGTGAAAGCATTGATGGGATGAACGAGGTGGAAGGGGAGAGAGTAAGGAAGGATGCTGATGAGGAAAAGTGGTATTTGGAAGAGAAGGGTATTGATAATGAGGATGAGAATACTTTGTTTAAAGGGATTGATAACGCAGCCGAGGGAAAGAGTAGTGGAAGTGGTGAGTTTCGTAGCCACATTGATCAAGCTTGGAACATGAAAGAGGATGAAGGTGATGTTTTTGATTTAAAAGAGGATGATGTGGGTAGGGAGGAAGTTGGGGAACTCGATGTTGTGGATGGTGAGACGAAAGAGGATGTGGAGAAGCTTGAGAAGGAAGAGAAAGAACTCACTGTCATTCTTGAAGGACCAAAACGTGCTTTTGGTGATTTGATTGCTGCTTCAGGAATCACGGATGAAATGCTTGACAGTTTGATTGCATTGAAAGATTTTGAAGGGATTGATGGGTTGCCCCCTCTTAGGGTAATAGAAGATATACGCTATGAGAAGAATACTAGAAAATCCACCAGAGCTGAAATGGAGCGTCTAAAGCAAGAGGAAGCAGCAAAAGCAAGAGTGAGACAAGTAGATGATAAAGGACGTGCTTATGGAACAGGCAGGAGAAAATGCAGTATAGCACGTGTCTGGGTTCAGCCCGGTAACGGTAAATTTATGGTTAACGATAAAGAGTTTGATGTCTATTTCCCTATGATCGATCACCGTGCTACTCTCCTTCGACCTTTCTCTGAGACAAAGACATTGGGGCTCTGGGATGTTAACTGTACTGTGAAAGGTGGTGGTGTTTCGGGGCAAGTTGGAGCTATACGATTGGGTGTTAGCAAGGCTTTGCAAAACTGGGAACCAGAATTGCGACCTGCACTTAGAAGCGTCGGCTTCTTGACAAGAGATTCAAGAGTGGTAGAAAGGAAAAAGCCTGGAAAAGCAAAAGCAAGAAAAAGTTACCAATGGGTCAAGCGTTAATCAAGTATATTATTCTGTGGGTGGCAATTTTTGTATTTCATTGATCCTGTACTGCATCGTATGTTTGGATTTCAACGACACTAAGGATAGAAGAAGATTTGTGTTATGAGTCATTCACCCTTTCCAGAGAGCTACTAGTTGCTGTTTTTGTTATAGTAGTCCTAACTTCATAATGAATGTTGTTTTAGGGATATGTTATATCCATTTTGGTTATTTTTGTGTAGTCATGTTTTTGATATACATGTATTTCTGATAAAAAGTGAAACATTCCATGTTTTATTTTTTTGGGAAAATAAAGGGAACCAACATCGTTCTTAAARAAAAAG
TARGET=327,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04508 Ps_contig_mira-and-tgicl-ass_franssen_33497
SEQUENCE=GTAGCAGCATCGGCCGCGGGAGGTGTTGCTGCCGCTGATGCTGTTCCTGTTGGTGTTGGTGTTGGTGTCAGTGTCTCACCAGTGAGTGTAGGTTTGGATGGATCAGTCAAGAAGAAAAGGGGTAGACCAAGGAAATACGGGCCAGATGGGTCTGTCAACATGCCGTTGTCACCATTGCCTATCTCATCTTCGGCCCCCCCTTCTTCTAATGACTTTTCGTCTGTGAAGCGAGGGAAACCGCAGGGGATGGAATACAAGAGGGCGAAAAAAGTTGTGATGGATCATCTTGGTGAGATGAATGGACACTCTGATGGCACAAACTTTATGCCGCATTTCATCACTGTCAATGCTGGCGAGGACATTACAAATAAGGTAATATCGTTTTCGCAACAAGGGCCGCGGGCAATATGCGTCTTATCGGCTAATGGTGTGATTTCAAATGTTACACTCCGTCACCCAGATTCTTCTGGGGGTACTTTGACTTACGAGGGTCGGTTTGAGATACTTTCCTTGGCTGGATCATTCATGCCTACTGACAACCAAGGAACAAGAAGCAGGGCAGGCGGAATGAGCGTCTCGTTGGCAAGCCCTGATGGCCGCGTAGTAGGCGGTGGAGTTGCTGGTGTTCTTATAGCTGCCACTCCTGTGCAGGTGGTGGTGGGAAGTTTTCTACCAAGCGGCCAAGAACAGAAACTAAAGAAGCCAAAGGCTGCTGATTATGCAGCAGCCACATTTACTCAAGCGACCGAAGTATCCGCAGAGCCGCCACCAGCCCAACCTAGTAATGCTGAAAAAGAGGATGTCATGGGTGGCGGTGGACATGGCGGTGGACATGTACTTCAAAATTCTGGAACCACAACCACCCTTAACTCAAATTTTACCTCTCCTCCACCTCCCACCGCCTTTCGAAGAGACAACTGGGTCAACATGCACGCCATGCCGGACACAAGAAAGTCCCCTACTGATATAAACATATCTTTGCCTGATAGTTAAGTCGATGATCGCCTTGCAAATTGTTTGGCCTGACGAAACTTGGCACCGAGATGTTTTATTTTATTTTATGTTACAATGTTCTCTCTATTATCTTAGATTTGGTTTAAACATGTTTGATATCTGTTACTATAGGGTGTAGTTGACAGTGTTGGTAGTTGCAGCTAGGTAGAGTATGAGTATCTCCGATTTTTCGGCTGATTAGGTAGATACAGCACAATGAATGGATTATCATGATGATGAGCAGGCTAATTCTGATGTAATGTTACCTTGTTTGCCTATTTACTTAGGACTGCATTTGTTNAACG
TARGET=1047,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6894
PRIMER_LEFT_PENALTY=0.161311
PRIMER_RIGHT_PENALTY=0.528040
PRIMER_LEFT_SEQUENCE=ATTTTACCTCTCCTCCACCTCC
PRIMER_RIGHT_SEQUENCE=AGCTGCAACTACCAACACTGTC
PRIMER_LEFT=874,22
PRIMER_RIGHT=1155,22
PRIMER_LEFT_TM=59.839
PRIMER_RIGHT_TM=59.472
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.7208
PRIMER_LEFT_1_PENALTY=0.281044
PRIMER_RIGHT_1_PENALTY=0.439723
PRIMER_LEFT_1_SEQUENCE=CTTTCGAAGAGACAACTGGGTC
PRIMER_RIGHT_1_SEQUENCE=TACCTAATCAGCCGAAAAATCG
PRIMER_LEFT_1=902,22
PRIMER_RIGHT_1=1197,22
PRIMER_LEFT_1_TM=60.281
PRIMER_RIGHT_1_TM=60.440
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=0.8091
PRIMER_LEFT_2_PENALTY=0.281044
PRIMER_RIGHT_2_PENALTY=0.528040
PRIMER_LEFT_2_SEQUENCE=CTTTCGAAGAGACAACTGGGTC
PRIMER_RIGHT_2_SEQUENCE=AGCTGCAACTACCAACACTGTC
PRIMER_LEFT_2=902,22
PRIMER_RIGHT_2=1155,22
PRIMER_LEFT_2_TM=60.281
PRIMER_RIGHT_2_TM=59.472
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=0.8178
PRIMER_LEFT_3_PENALTY=0.281044
PRIMER_RIGHT_3_PENALTY=0.536763
PRIMER_LEFT_3_SEQUENCE=CTTTCGAAGAGACAACTGGGTC
PRIMER_RIGHT_3_SEQUENCE=CCGAAAAATCGGAGATACTCAT
PRIMER_LEFT_3=902,22
PRIMER_RIGHT_3=1186,22
PRIMER_LEFT_3_TM=60.281
PRIMER_RIGHT_3_TM=59.463
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=1.1239
PRIMER_LEFT_4_PENALTY=0.161311
PRIMER_RIGHT_4_PENALTY=0.962561
PRIMER_LEFT_4_SEQUENCE=ATTTTACCTCTCCTCCACCTCC
PRIMER_RIGHT_4_SEQUENCE=CCAACACTGTCAACTACACCCT
PRIMER_LEFT_4=874,22
PRIMER_RIGHT_4=1144,22
PRIMER_LEFT_4_TM=59.839
PRIMER_RIGHT_4_TM=59.037
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_04509 Ps_contig_mira-and-tgicl-ass_franssen_33498
SEQUENCE=ATGGGAGGAATGGGGGAATCACCGAAATCTGAGAGAAGAAATATTCATGGGATTGATGTTAGATGCAAAGTGGTAGATTTTGGAAATGCTTGCTGGGCTGATAACCCTTTTGCGGAAGAAATTCAGACAAGACAGTATAGAGCTCCCGAAGTTATACTGCAAGCTGGTTATTCGTTATCCGTCGACATGTGGTCTTTTGCTTGTATTGCTTTTGAACTCGCCACTGGTGATATGTTGTTTACTCCCAAAGTCGGGCAAGGTTTTAGCGAGGATGAGGATCACCTTGCTCTGATGATGGAACTCCTTGGAAAGATGCCTCGAAAGATTGCTACTACAGGAGCGAAATCTAAGGATTTCTTCGACAGACACGGTGATCTGAAAAGGATTCGAAGGCTAAAGTTTTGGCCACTAAACAAACTATTGATCGAAAGATACATGTTTTCAGAGAGCGACGCTCTTGAATTTTCGGAGTTTCTTGTACCTCTTCTTGATTTTGCGCCCGAGAAGCGACCAACTGCGCAGCAGTGCCTACAACACGCGTGGCTCGCAGGAAATGACTCAGTTCCTAGTGAAATGACAAATGAAACTAGTGTTGAAAACGTGGATGCTGGAATAAGCAACCTTAAAATCAGGGTGGGGAAGTGAAGTGAAGTGAAGGGGAGATAGGTATTTGAGAATCACCCTTCTTACAATATTRTTGATTTTTTTATTGTGATTTTATTT
TARGET=639,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04510 Ps_contig_mira-and-tgicl-ass_franssen_33499
SEQUENCE=AGTACGNRRGATCTCTAAAAAAACAACACTATTTTCCTCTGTAAAAGTCAACCGTGAATGCCCCAATCCTTCCAGAAAGCACCGGCATTATCCGTCTGTACTTTTCATAGGGAAACGATTGAAGTTAAGTGANCAGTCACATCACATCACATCACATCACATGGCAACCCAAACCGAACCAAAGAAGATCATAATTGACACCGACCCTGGAATCGATGATGCTATGGCTATATTCCTTGCTCTGCGATCGCCAGAGGTACAAGTTATTGGACTTACAACTATCTATGGAAACGTTTACACTACTCTTGCTACCAGAAATGCCTTGCATCTGTTGGAAGTTGCTGGAAGAACTGATATACCAGTTGCTGAAGGATCCCATGTCACATTAACTAAAGGGACGAAACTTCGCATTGCAGATTTCGTTCATGGTGCAGATGGACTTGGCAACCAAAACTTTCCTCCACCAAATGGGAAACCCATTGAAGAATCAGCTGCTGAATTCTTGGTTAACCAAGCGAAAGCTAACCCTGGAAAAGTCACTGTGGTGGCGTTGGGCCCTCTTACAAATATTGCTTTGGCTATACAGATGGACCCGGAATTCGCTAAGAACATCGGGCAAATTGTTCTGCTTGGTGGATCTTTTGCAGTAAACGGCAATGTGAATCCAGCTGCTGAAGCAAATATATTTGGTGATCCAGATGCTGCTGATGTTGTATTCACAAGTGGTGCAGATGTATTGGCAGTTGGAATAAATGTTACTCACCAAGTCGTATTGTCAGGTTCTGATCGAGAAAAGTTAGCAAGTTCAAAAGGAAAATTTGCTCAATACCTGACCGGAATCTTAGAGGTGTATTTCTCTTACCATTGTGACGCATACAACACCAATGGAGTTTACCTTCATGACCCAACAGCGTTGCTTGCAGCCGTTGATCCTTCACTTGTAACTTGCACAGAGGGTGCTGTTAGAGTCCAAACCAGTGGCATAACAAGGGGACTTACAATAATCTACAATAAACAGAAAAGGTTTGAAGAAGTCACTGAATGGTCCAATATGCCTACAGTAAAAGTAGCTGTGACAGTTGATGCTCCTAGAGTTGTGAAATTGGTCATGGATCGTCTTGTGGCTTGAAGTAGCTATGGAGACGTTCAATTAACATTATTGAGTATTGTCCATCTACTTAAATCTGGTTTCAATGTTTTAAACATAAGACTATGCTCACTTCAAAGCTGTTCTGTTTATTCTTCGCGCCATAATTGTGTTTTGGAGCTCTTGAGCATTACTTTTTACTTCACATTTTAGTTCAATTTCAATGTAAATTAACATGTGTATCCTAATTATTATATATTGAGTCTCATTCTTGTAGAAAACTGGATGATGCCTTTCTATTAGACAGTACTCATCTAATATTGATTGTTGGAAGCATCATAACATGTTAAAAAAAGTATCTCTGATCTTGTGC
TARGET=137,25
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04511 Ps_contig_mira-and-tgicl-ass_franssen_33509
SEQUENCE=CTAAGCTTCCTCTTTGGAACAAGCACTTCTTTCTTACCAGGTACTTCTACTTCAAGCTTTCTTCTTCTTCTTCTCAAATATTTCAATTTTACTGTCTCTGTTAGTGATTTTTGTTAGTAGATTGAAGGAGCAGCATTTGAAGATGGTAAAGGTTTAAGCAACTGGGATGTATTCAGTCACACACCAGGGAAGATAAACAATGATGAGAATGGTGATGTAGCAATTGATCATTATCATCGCTATTTGGAAGATATTAAATTGATGTCTTCTCTTGGGGTAAATGTTTATAGATTTTCAATTTCATGGACTAGGATTCTACCTAATGGGTTATATGGAGACATAAATCCAATTGGGATTATGTTCTACAACAATCTTATTGATAATCTTTTGCTTAGAGGAATTGAGCCACTTGTGACAATTCACCATCACGACTTGCCACATGAGCTGGAAGAAAGATATGGTGGTTGGCTCAGTCCCCTAATTCAGAGAGATTTTGTTCATTTTGCTGAAATTTGTTTTAAAAGTTTTGGCGATAGGGTTAAGTATTGGGCTACCATCAATGAGCCAAACTTGGTTTCAGACTTTGCATTTTTAAGGGGAGCATACCCTCCTTGTCACTGTTCCCCTCCTTTCGGGAACTGTGATACCGGTAACTCTGATGTTGAGCCTCTCCTTGTCATGCACAATATGTTGCTGTCGCATGCTAAGGCTGTTGAATTATACCGCAAGCACTATCAGGAAAGTCAAGGTGGAACCATTGGTATTGTTGCACATACCTTCATGTATGAACCTCTCAGAGATGAAGAATGTGATAGACAGGCTGTAAATAGGACCTTGGCTTTTGTCGTAGACTGGTTACTGAATCCCCTAGTTTTTGGTGAGTACCCTGCTGAGATGCGCTCTATTCTTGGGAACCAGTTGCCAAGGTTTTCGCCTAAGGAGAAGAGTCTCCTAAGAGGAAGCTTGGACTTCATTGGCATCAATAACTATGGAGCTCTCTATGCCAAGGATTGCTTTCTCTCTACTTGTCCTCTCAATTCTTCTCGACCAATAAGAGGCTTTGCGGAAACAACTGGAATGAGAGACGGCATTCCAATTGGTGGTCAGACAGGAATTCCAAGGTTCTTTGTGGTTCCAGAGGGCATGGAGAAGATTGTAGACTACATAAAGATTAAATACCATAACATGCCCATGTATATAACAGAAAATGGATATTCTTCGCCACTCAACCAATCTACGACAATGCATGATACGTTACACGATTTCAAACGGATAGAGTATCATAAAGCTTACCTTGCAGCTCTGCTTAAAGCCATAAGAAAAGGTGCAGATGTAAGAGGATACATGATATGGTCATTGTTTGACAGCTTTGAATGGAATAGTGGATATAATATAAGCTTTGGACTTTATTATGTGGACAGGAACACACTTGAAAGAATTCCCAAACTTTCTGCTCAATGGTTTTCTGATTTCCTCAAAAGTACTAGTGATAGCAACATAAAAGACTTGAGCGAGAAGAATAGAAGCAATTATGTGACAACTGCTGTTGTCTAAGTTATTTGAAATTGATCATGTAATTCAATATCAAATACATCGGTGATTGTTAACATYATACTATTTATTATTTGTGAAACTTTAATTTCTAGCTTAATAGAAAACACTCT
TARGET=59,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04512 Ps_contig_mira-and-tgicl-ass_franssen_33510
SEQUENCE=CCGGTTCCTTTCCCGGTAACCCGAACCCCCTACCGTTCCCGTAAACTACTTGCNGGTTTGGGTAGTATTGTCACCCTCGCAACCCAACCCTACGTGGTACTGAATTCCCATATGCTCCCATAACTGATGGACTTATACTAGGCTGAGCACTGTATCCACTCTGAACTCCATGACCTGCAGCAGAAACACCTCCATGATTAAGAGCATTACTCGTTCCAAGACTCCCCAACAAATCACTCAGCCCCAAAGTAGCACCCTGAGATGCAAGTAGCGCCGTCAAAGCCTGTCCAAGTGCAGGCGTCAGCACCGGGTTCAGCCCCTGTGCAGCAGAAGCAGTAGCCTGGTTGTACCCAATTGTTGGTCCTGCCGGCGCCATTAAGTGCCCTGGTTGGCTAACTGCAACACTACTACCACCGGCATATCCAATATTGTCATTTCTCTGAAATTGTGTCCTCTGAGTCAATGGGTTCAGTTGGTTCAATTGATTCAATGAATTCACGTGTTGTTGCTGCTGTGGTTGTTGATGTTGTGTCTTCGCCTTAGGACCATCAATTGCCCTCTGGCAATGCAAAATATGTCCCTCAAATTCCTTATGAGGCTCTTCCAATGCCCTCCTTGCACTCTCAGCACTCTTATAAACAAACAAGCAAAACCCTTTCGGCTTTCCCGTCGCCTTGTCAAGCCCTAACGGCCCTTCCTCAATTTCTCCAAACCTCGAGAAATAAGCAAACAGTTTCTGCGGATCCAATTCAGGACCAACATTGCTGATATATATCTTCCTCTGCGTATACTCCGAGCCTTGCGGCGCCAACTGCGCCGTTGGCTGCGGAGTTTGCTGCACAGGACCAATAGACGCCAGCTGGCACGCCGTCATCCTGTTCCCAATCTTCTTCTGAGGCTCTTTCAAAGCATTCCTCGCCCCACTCCGCTTCTTGAAAAGAATAAACCCATAACCCTTAGATTTCCCAGAAACCTTATCAGTAACAGCTTTACAATCCTCAATTTCACCGTATTGCGAAAAAGCGTTAATTAGGGTTGCGGAAGTGGTGTCCCATCCCAAACCATGAACAAAAATCTTCCGGTGTGACGCGTCTCCGTCAGCAATCTTACGGATCCGGTCCGCAACATCACGATGTTTAGACGCCGCCTCGCAAAGGAGATTCACGATCTGCTCTTTGGTGAAAGGCTCCACGAGGTCCTTAATCGGTTCATCGTCTTCTTCTAGGTATTCTCCACCTTGAGCTTGTTCTCCTCCGTCGTCCTCCTCCTCCTCCTCTTCTTCCTCCTCCTCCTCCTCTACTTCCTCAACCTCTTCATATTCCTCCTCAACTTCTTCATATTCTTCCTCAACTTCTTCGGCGGGGATATAGGGTTCCGGTTCGGGATCCGGTTGAGGCTGAGAGTGGTGCTGTTTGAGCGGTGGTTCCTCTGGTTGAGACGATTTAGCGACGAGCTTTCTCTTTCTCACCATTGTTTTGTTCTTCGCGTGAAGTGAAGGTGGTAACTAGGGTTTCAGATTGGGAATTTAACACATTCACCACACTACAACATGTCTCCCGACGAC
TARGET=1260,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0529
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=CCTTAATCGGTTCATCGTCTTC
PRIMER_RIGHT_SEQUENCE=ATGGTGAGAAAGAGAAAGCTCG
PRIMER_LEFT=1197,22
PRIMER_RIGHT=1470,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.0529
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=TCCTTAATCGGTTCATCGTCTT
PRIMER_RIGHT_1_SEQUENCE=ATGGTGAGAAAGAGAAAGCTCG
PRIMER_LEFT_1=1196,22
PRIMER_RIGHT_1=1470,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.1005
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=CCTTAATCGGTTCATCGTCTTC
PRIMER_RIGHT_2_SEQUENCE=AGAAAGAGAAAGCTCGTCGCTA
PRIMER_LEFT_2=1197,22
PRIMER_RIGHT_2=1464,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.1005
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.066394
PRIMER_LEFT_3_SEQUENCE=TCCTTAATCGGTTCATCGTCTT
PRIMER_RIGHT_3_SEQUENCE=AGAAAGAGAAAGCTCGTCGCTA
PRIMER_LEFT_3=1196,22
PRIMER_RIGHT_3=1464,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=0.1884
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.154341
PRIMER_LEFT_4_SEQUENCE=TCCTTAATCGGTTCATCGTCTT
PRIMER_RIGHT_4_SEQUENCE=GAAGTAGAGGAGGAGGAGGAGG
PRIMER_LEFT_4=1196,22
PRIMER_RIGHT_4=1302,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.846
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=59.091
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=107
=
PRIMER_SEQUENCE_ID=SSR_04513 Ps_contig_mira-and-tgicl-ass_franssen_33510
SEQUENCE=CCGGTTCCTTTCCCGGTAACCCGAACCCCCTACCGTTCCCGTAAACTACTTGCNGGTTTGGGTAGTATTGTCACCCTCGCAACCCAACCCTACGTGGTACTGAATTCCCATATGCTCCCATAACTGATGGACTTATACTAGGCTGAGCACTGTATCCACTCTGAACTCCATGACCTGCAGCAGAAACACCTCCATGATTAAGAGCATTACTCGTTCCAAGACTCCCCAACAAATCACTCAGCCCCAAAGTAGCACCCTGAGATGCAAGTAGCGCCGTCAAAGCCTGTCCAAGTGCAGGCGTCAGCACCGGGTTCAGCCCCTGTGCAGCAGAAGCAGTAGCCTGGTTGTACCCAATTGTTGGTCCTGCCGGCGCCATTAAGTGCCCTGGTTGGCTAACTGCAACACTACTACCACCGGCATATCCAATATTGTCATTTCTCTGAAATTGTGTCCTCTGAGTCAATGGGTTCAGTTGGTTCAATTGATTCAATGAATTCACGTGTTGTTGCTGCTGTGGTTGTTGATGTTGTGTCTTCGCCTTAGGACCATCAATTGCCCTCTGGCAATGCAAAATATGTCCCTCAAATTCCTTATGAGGCTCTTCCAATGCCCTCCTTGCACTCTCAGCACTCTTATAAACAAACAAGCAAAACCCTTTCGGCTTTCCCGTCGCCTTGTCAAGCCCTAACGGCCCTTCCTCAATTTCTCCAAACCTCGAGAAATAAGCAAACAGTTTCTGCGGATCCAATTCAGGACCAACATTGCTGATATATATCTTCCTCTGCGTATACTCCGAGCCTTGCGGCGCCAACTGCGCCGTTGGCTGCGGAGTTTGCTGCACAGGACCAATAGACGCCAGCTGGCACGCCGTCATCCTGTTCCCAATCTTCTTCTGAGGCTCTTTCAAAGCATTCCTCGCCCCACTCCGCTTCTTGAAAAGAATAAACCCATAACCCTTAGATTTCCCAGAAACCTTATCAGTAACAGCTTTACAATCCTCAATTTCACCGTATTGCGAAAAAGCGTTAATTAGGGTTGCGGAAGTGGTGTCCCATCCCAAACCATGAACAAAAATCTTCCGGTGTGACGCGTCTCCGTCAGCAATCTTACGGATCCGGTCCGCAACATCACGATGTTTAGACGCCGCCTCGCAAAGGAGATTCACGATCTGCTCTTTGGTGAAAGGCTCCACGAGGTCCTTAATCGGTTCATCGTCTTCTTCTAGGTATTCTCCACCTTGAGCTTGTTCTCCTCCGTCGTCCTCCTCCTCCTCCTCTTCTTCCTCCTCCTCCTCCTCTACTTCCTCAACCTCTTCATATTCCTCCTCAACTTCTTCATATTCTTCCTCAACTTCTTCGGCGGGGATATAGGGTTCCGGTTCGGGATCCGGTTGAGGCTGAGAGTGGTGCTGTTTGAGCGGTGGTTCCTCTGGTTGAGACGATTTAGCGACGAGCTTTCTCTTTCTCACCATTGTTTTGTTCTTCGCGTGAAGTGAAGGTGGTAACTAGGGTTTCAGATTGGGAATTTAACACATTCACCACACTACAACATGTCTCCCGACGAC
TARGET=1281,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0529
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=CCTTAATCGGTTCATCGTCTTC
PRIMER_RIGHT_SEQUENCE=ATGGTGAGAAAGAGAAAGCTCG
PRIMER_LEFT=1197,22
PRIMER_RIGHT=1470,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.0529
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=TCCTTAATCGGTTCATCGTCTT
PRIMER_RIGHT_1_SEQUENCE=ATGGTGAGAAAGAGAAAGCTCG
PRIMER_LEFT_1=1196,22
PRIMER_RIGHT_1=1470,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.1005
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=CCTTAATCGGTTCATCGTCTTC
PRIMER_RIGHT_2_SEQUENCE=AGAAAGAGAAAGCTCGTCGCTA
PRIMER_LEFT_2=1197,22
PRIMER_RIGHT_2=1464,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.1005
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.066394
PRIMER_LEFT_3_SEQUENCE=TCCTTAATCGGTTCATCGTCTT
PRIMER_RIGHT_3_SEQUENCE=AGAAAGAGAAAGCTCGTCGCTA
PRIMER_LEFT_3=1196,22
PRIMER_RIGHT_3=1464,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=0.1968
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.066394
PRIMER_LEFT_4_SEQUENCE=GGTATTCTCCACCTTGAGCTTG
PRIMER_RIGHT_4_SEQUENCE=AGAAAGAGAAAGCTCGTCGCTA
PRIMER_LEFT_4=1224,22
PRIMER_RIGHT_4=1464,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.934
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_04514 Ps_contig_mira-and-tgicl-ass_franssen_33516
SEQUENCE=TGGCGTCGCTTTCCACTNGACAGCAAAAAGTTAGAGAGAAAGACTGAGAGAGAGAAACGAAGTCAAGCTTCAATCACTGTGATCGAAATCACTCCGATTCCGATTCCGATTCCGATTCGGAGAATGGCTTCTTCGAGCACAGCAACTCCTGGATCTGCTGATTCCACCGCTACAAGAAGACACACCAAGCGACCTAAGTATTCAAAGTTTACCCAGCAAGAACTTCCTGCATGCAAGCCTATTCTCACGCCACGAGCGGTTATTTCAGCGTTTTTGCTGGTTAGTGTTGTGTTCATTCCTATTGGAGTTGCTTCACTAATGGCTTCAAGAAAGGTTGTTGAAATCGTTTATAGATATGAGTCAGAATGCTTACCGAGTAATATTACCAACAAGGTAGCATATATTCAGAATCCTACAACGGATAAATCTTGCAACATAACACTGAATGTGACCAAGCACATGAAGGCACCTGTTTTTGTATACTATCAGTTGGACAACTTTTACCAGAATCATCGCCGGTATGTTAAAAGCCGAAGTGATGAGCAATTGAGGAATCTTAAGGATGAGAAGGAAGTTAGTACTTGTAAGCCTGAAGATTTTGTCAATGGTAGTGCAATTGTACCTTGCGGTCTCATAGCTTGGAGTTTATTCAATGACACATACAGCTTCTCTATCAAAGGAACGAATTTGAAAGTAAACAAGAGTGGCATTGCTTGGAAGAGTGATAGAGAGCACAAATTTGGAAAAGATGTTCACCCCAAAAACTTTCAAAGCAGTTCTATAATAGGTGGTGCCCATCTTAATGAATCCATACCGCTGAGTGAGCAGGAGGATCTTATTGTCTGGATGAGAACCGCCGCCTTGCCAACTTTCAGGAAGTTATATGGAAGAATAGAGGTGGATCTGAAATCCGGTGRTATAATCAACGTAACACTTAAAAATCAGTACAACACATACAGTTTTAATGGCAAAGAAAAAAC
TARGET=46,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04515 Ps_contig_mira-and-tgicl-ass_franssen_33520
SEQUENCE=TGAGATCTTAGGGGTCCCGTTTGGTTGGGAAGTAGTTTGGTTGATGATATGTTTGGTAGCTTTTGGGTAAGGAGGAGGAGGAATGCATATGAATCATGGTGTACCGAGGAAAGCTGCTGCTATCGAGAACAAGTTGAGTTGCACTCTTGAGGAGATTTATAAAGGAACTACAAAGAAGATGAAGATCTCTAGAGAAATAGCTGATGCCAGTGGCAAAACCATGCCAGTGGAAGAGATATTGACCATAATTGTGAAGCCTGGTTGGAAGAAAGGCACAAAGATCACCTTCCCAGAAAAGGGCAACGAGCAGCCGAATGTARCCGCCGCAGATCTTGTATTTGTGATCGACGAAAGGCCGCACAGTGTGTTCAGTCGCGAAGGAAACGACCTGATTGTGACTCAGAAGATATCTCTTGTGGAGGCGTTAACTGGTTATACAGTGCACCTCACTACACTGGATGGTAGAAACTTGAGCATACCAATAAACAATGTTATTCATCCCAACTATGAGGAGGTTGTTCCAAAAGAAGGGATGCCACTTCCTAAGGATCCATCAAAGAAGGGGAATTTGAGGATAAAGTTTAACATTAAGTTTCCAAGTCGGCTCACTGATGAGCAAAAAGTAGGAGTTAGGAAACTCTTGGGTGCAATCTGAGATTGAACATAAATGGATTGAGAATAGTCTATAGTTGGTGGAAGATATGCCACCTGAAAATATTAGATTTACCAATAAACTTGAATTGTTTTTTTTTTGTTATTGTATGGG
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04516 Ps_contig_mira-and-tgicl-ass_franssen_33524
SEQUENCE=GTTATCAAAACACATCATGCCAACACATAATGAATTAAAATTCCTGATATAAATGAAGAACAAATACATTTTGTAACTGAGTATCAATAACAACTTATGCTAAAATCTTTCTGAAAACCCAAAAAGCTTATTTTATTTCAAACAATTAATGACACTCAAATCTATTTCAATTTTTGCCTTGAAGCTTCAAGGTAAATGCCTTCAAAGACAAAAGAACTTCCTTCAAATTATTCTTAGCACCTTCATCAATCAAGTCACCATCTCCGTTAAATTTTGCCGGAGGCTGAAACGCGTTGAGGAAAAACTCGGGTTTATTGATGAAATGAAGATCGAGAAAAACTCCAACTTGTCGAAGATGGTAGTGCGATTTCGCACCACCATGACCTCCTCCAACACTCACCACAGCAGCAGGTTTACCAGCCCAAACATTTGGTGGCCTTGATGCCCAATCAATTGCATTCTTAAGTGGAGCAGAGAGAGAATAGTTATATTCAGGAGAAGCGAAGAGAAAGGCGTCAGCCGCAACAATCTTTTGGCGAAAAGCTTCGATTTCAGCGGGATAAGTTCCTTCTTTCTCAAGATCAGTGTTCAACAATGGCAGATTTGAAATATCAACATGTTCAATTTCAACACCCTCGACAACACTTTTGCTCAATTCAATAGCAGCACGAATGAGACCGGTGTTGTTAGAAGCTTTTCGAATAGAACCAGAGAGAGCGACGATCTTGATAGCAGCAGCCATATTAGAACTGTGATTTGTCAATTGGAAGAGAAGAGTATTGTATTGTATTTATATATTGTAGTTGTGAGATCCGTGTGTAATCNCTACNNTNCRNRRTACNANTGYNTAAAATTCAATATTGGAAAAGATTAGAAT
TARGET=777,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04517 Ps_contig_mira-and-tgicl-ass_franssen_33525
SEQUENCE=CCAACCATTCCTATAACGTANACCAAACCTTAAAAATACTACGTTTTCCTTATTGTCTTTCTTTCCTACTACCCTACTCTAACCTCCCAACAATACACAAGCTCTTTCTCAACAATTATCATTAGTCTCAACATTGTTGTTATGAACTTTGTACTCATGCTTCATGGCCAAGAAAAGAAAAAGTACAAAATTCAACACTCCAAGCATTGCTAGCAGCCAATAGAAGTCATCTAACCTACCCTTGTCAAGATTACTCTTCAACCATCTTTTCTTGCTTACTTTGTCCACAATTGACACCAATAAGCTACTCACAAAAAATCCCATTGAAAGAGTAGTTAGAAAAAAACCAGTACTCATAGATTTCATTCTCTCAGGTGCTTCCCTTATGAAAAACTCTAACTGTCCAACATAGGCAAAAGCTTCTCCAGCACCAACTAGAAAAAACTGAGGTACAAGCCAAAAAGCACTTATTTTATTGTCTTTTTTAACTGCATTGTCCCTTCTTTCTTTCTCAATAATTGCAGAAACTGCCATTGCAACAATGGAGAAAATAAGTCCAATTCCAACTCTTTGAAGGCTTGTGAGTCCTTGAACATTGTGAGTGAATTTTCTTGCTAAAGGTACAGTGAGTTTCTCATTTAGGGAAGTGAAAAGGAGAATTGTGATGAAGAGAAAAGCTGATAAGGATCCTGATGGAATATCTAGAGATCCTACTTTTCTGTTCATGAATGTTGCTTGCTCAATAGTGAAGGTGTTCATTTGTGAGTAAACTGTCCAAAAGAGGATGCATGTTGACCAAATGGGAATGAGATTGATTACCATTTTAACCTCTTCAACTTGAGTCATTGTTGAAACCAACCATGGACTTTGTTTGTTTTCATCATTTGAGTTTGTCTCATCTAGGATTGCAGCTTTGTCAAGAGACCTTAATCTATCTGTATGTGGGACCTTAGCTTCAGCATAACCATGGAGTAAACAAGGTTCTGAAGGTAGAGGAAGAGTCCTCTTTTTCCAAGCCAAGAACAATACTCTCCATATAACAGAAAAAGGACTTCCTTGTGGTTTCTTGAATCTATAAAATGGAGTACCGCAAAGCAAAATACAAACTGCAACCACCATTGTTCCTGCGGAAATTCCATATCCCCAACCTCTTCCTATGTTGTCTTGAACATAGACCAACACAATAACTGAGAATAAGGATCCAATACTGACGAAAAAGTAGAATCTGTTGAAGAAAAATATCATTTTCTTTTCTTCTTTTGGATCTCTTGTGTCAAATTGATCGGATCCGAAACCGGATACATTGGATTTTATTCCTCCGCCGCCTACAGCTATTGTGTAAAGTGCTGCAAAGAGAAGTACTAACTGTTTTCCGGAGGCGGGAATGCACTCGTGGTGTTTTCTTCTTACTTCACTGCATGGAGGTGGTAGCATGCTTGGAATTGTTGTAGCTAAAGTTAACAAACACACTCCCATTGCTGCTATGGATGCAAATATCACAACAGTGACATATCTGCCAAGCTTTGCATCAGCTAAAAAACCACCAAGAAGGCCAAGCAAGTTGAGTGTTCCCATAAAATTAGTAACAATGGTAGCAGATTTAGCTAGAATCAAAGATGACAAACTCCACCAAACAAATAACGTCCGAAACCCTAAGGTTTACG
TARGET=501,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2615
PRIMER_LEFT_PENALTY=0.087031
PRIMER_RIGHT_PENALTY=0.174518
PRIMER_LEFT_SEQUENCE=ACATAGGCAAAAGCTTCTCCAG
PRIMER_RIGHT_SEQUENCE=ATGTTCAAGGACTCACAAGCCT
PRIMER_LEFT=406,22
PRIMER_RIGHT=595,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=60.175
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=0.3069
PRIMER_LEFT_1_PENALTY=0.087031
PRIMER_RIGHT_1_PENALTY=0.219884
PRIMER_LEFT_1_SEQUENCE=ACATAGGCAAAAGCTTCTCCAG
PRIMER_RIGHT_1_SEQUENCE=CAATGTTCAAGGACTCACAAGC
PRIMER_LEFT_1=406,22
PRIMER_RIGHT_1=597,22
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=59.780
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.3247
PRIMER_LEFT_2_PENALTY=0.087031
PRIMER_RIGHT_2_PENALTY=0.237699
PRIMER_LEFT_2_SEQUENCE=ACATAGGCAAAAGCTTCTCCAG
PRIMER_RIGHT_2_SEQUENCE=AGCCTTCAAAGAGTTGGAATTG
PRIMER_LEFT_2=406,22
PRIMER_RIGHT_2=578,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=59.762
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.3247
PRIMER_LEFT_3_PENALTY=0.087031
PRIMER_RIGHT_3_PENALTY=0.237699
PRIMER_LEFT_3_SEQUENCE=ACATAGGCAAAAGCTTCTCCAG
PRIMER_RIGHT_3_SEQUENCE=CAAGCCTTCAAAGAGTTGGAAT
PRIMER_LEFT_3=406,22
PRIMER_RIGHT_3=580,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=59.762
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.3492
PRIMER_LEFT_4_PENALTY=0.174657
PRIMER_RIGHT_4_PENALTY=0.174518
PRIMER_LEFT_4_SEQUENCE=GAGGTACAAGCCAAAAAGCACT
PRIMER_RIGHT_4_SEQUENCE=ATGTTCAAGGACTCACAAGCCT
PRIMER_LEFT_4=446,22
PRIMER_RIGHT_4=595,22
PRIMER_LEFT_4_TM=59.825
PRIMER_RIGHT_4_TM=60.175
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_04518 Ps_contig_mira-and-tgicl-ass_franssen_33530
SEQUENCE=AACGAAATCTTTCAAATCCTTCTTCTTCAGATCTAAAGATAACAGTGAATAAAACACCCTCTTTGATTCTGTTTCTAATTCTTCTTTCATTCGCAGCATTTTCATTTTCCCCTAATTTTTCCTCCAATTGAAACCGGTTATTGGAAATCCCGATGTCGGCTTCCACCGTGTCAATCACGGCGGCGAACCCGGGGACGCGTCGACGACCTGTGGTCATCACCGAGAAGAAAACCACCAGTAATATCGAACTCCTCGCTAACGACGTCACGGCTTCTCCAAATGCCATCGCTTCTGCCACCGATGACGGCAAGACTCCGTCTACCCTCGGTAACGGTCGAGACTTGAGCCATCACTCGATCCGCGGCGAGGCTGTTCTTTCCAAGGATTTGACGCCAGCATCAAAGAGGATTACTGGCGGGGATTCTGCTGCCGTTACGCCGCGGCGGTTGCGGAAGACCGTTGGAAAGTCCGACAAACAGCGGTGGGTTACCGTGGCCAGGATCTTTGCGAAGAATTTTGTGCTATTGGCGATGCTTGCTGGTTTGGTTCAGTTGATTAGGTGGTTTGCGTTGAAATCCGGTGAAGGAGTTGTCGGTGGGTACACTGGGTTGTCGGAATATGAGGACCGGATTTCGGAAATGGAGGGTTTGTTAAAGAAGACGACGAAGATGATCCAGGTACAGGTGGATGTGGTGGATACGAAGGTTGGAGGGTTAAGGAAGGAAATGGATGCGAAAATTGAGCAACAAGGTGCATTCTTGGAGAGTGAGTTGAAGAAATTGGTGACAAAGAGTGAGAAATTGGAGAGATATTTGGATGAGTTGAAAGTAGAGGATTTGTTAACAAAGGAGGAGTTTGAGAAATTTGTTGAGGGTTTGAGCAAGGGGAAGGGGAATAGTTATGAAGGTGCTAGTTTGGATGAGATAAGGGAATATGCAAGAGGTGTGGTTGAGACGGAGATTGAGAAGCATGCTGCAGATGGGCTTGGGAGAGTGGATTATGCCCTTGCAAACGGTGGAGCCGCAGTTGTGAGGCATTCGGATCCCTATGATATGCGGAGGGGAAAACTGTTCTCGTTGTCTCCTGGAAATGGTGTTCATCCCAATGCTGATAAGATGTTGAAGCCGAGTTTTGGGGAGCCCGGACAGTGTTTTCCGTTGAAAGGCAGCAGCGGGTTTGTTCAGATTAAGTTGCGAGCTGAAATTATTCCTGAGGCTATTACCTTGGAACATGTAGCAAAGAGTGTTGCATATGATAGATCTAGTGCTCCTAAGGACTGTAGGATCTCTGGTTGGCTGCAGGGGCGAAATACTGATTCTCTAATTGATACTGAAAAGATGTTTCTTCTGGCAGAGTTTACATATGATCTTGAGAAGAGCAATGCTCAAACTTTTAATGTGTTGAGTTCAGCAGGTTCTGGAGTTATTGACACAATAAGGTTTGATTTCACGTCAAATCATGGAAGTCCTTCACACACCTGTATATATCGCTTGAGAGTTCACGGTCACGAATCTGACTCAGTTTCTATGATGGCAATTGAGTCATAAATGTATCATTTGTTTGTTTGAAAGTAATTCATCTGTGTAATTGTTCAGTATTATTTGTTTAGATGTAGGTAGGAATCCATGGACTAAATATACTAGATGCAGTCAGTAATGTCGTTGTTTGTGTATTTAACTTCTTATTGCTGCTTCAAATGGATTATGTATTTAGTGTATGATTTCTGAATTAGGAAGATGATAGATTTCACACTTTTGGNNTTAANANAGNNTTGCTAATTTCTCTTTATTGTA
TARGET=1556,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3176
PRIMER_LEFT_PENALTY=0.046801
PRIMER_RIGHT_PENALTY=1.270774
PRIMER_LEFT_SEQUENCE=TGTGTTGAGTTCAGCAGGTTCT
PRIMER_RIGHT_SEQUENCE=CAAACAACGACATTACTGACTGC
PRIMER_LEFT=1396,22
PRIMER_RIGHT=1667,23
PRIMER_LEFT_TM=59.953
PRIMER_RIGHT_TM=59.729
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=1.3481
PRIMER_LEFT_1_PENALTY=0.077340
PRIMER_RIGHT_1_PENALTY=1.270774
PRIMER_LEFT_1_SEQUENCE=GAGTTCAGCAGGTTCTGGAGTT
PRIMER_RIGHT_1_SEQUENCE=CAAACAACGACATTACTGACTGC
PRIMER_LEFT_1=1402,22
PRIMER_RIGHT_1=1667,23
PRIMER_LEFT_1_TM=59.923
PRIMER_RIGHT_1_TM=59.729
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=1.4291
PRIMER_LEFT_2_PENALTY=0.158297
PRIMER_RIGHT_2_PENALTY=1.270774
PRIMER_LEFT_2_SEQUENCE=ATATCGCTTGAGAGTTCACGGT
PRIMER_RIGHT_2_SEQUENCE=CAAACAACGACATTACTGACTGC
PRIMER_LEFT_2=1483,22
PRIMER_RIGHT_2=1667,23
PRIMER_LEFT_2_TM=60.158
PRIMER_RIGHT_2_TM=59.729
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=1.6996
PRIMER_LEFT_3_PENALTY=0.022341
PRIMER_RIGHT_3_PENALTY=1.677241
PRIMER_LEFT_3_SEQUENCE=TTCACGTCAAATCATGGAAGTC
PRIMER_RIGHT_3_SEQUENCE=CAACGACATTACTGACTGCATCT
PRIMER_LEFT_3=1445,22
PRIMER_RIGHT_3=1663,23
PRIMER_LEFT_3_TM=59.978
PRIMER_RIGHT_3_TM=59.323
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=219
PRIMER_PAIR_PENALTY_4=1.7240
PRIMER_LEFT_4_PENALTY=0.046801
PRIMER_RIGHT_4_PENALTY=1.677241
PRIMER_LEFT_4_SEQUENCE=TGTGTTGAGTTCAGCAGGTTCT
PRIMER_RIGHT_4_SEQUENCE=CAACGACATTACTGACTGCATCT
PRIMER_LEFT_4=1396,22
PRIMER_RIGHT_4=1663,23
PRIMER_LEFT_4_TM=59.953
PRIMER_RIGHT_4_TM=59.323
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_04519 Ps_contig_mira-and-tgicl-ass_franssen_33531
SEQUENCE=GCGGTGCAGATCGAACTGCGCTGCCACCAAAATATTAAACACCACTTTTCCTTTTTTATTTCATTCTCCTACTTGCAGCACTGTTCTCCGCACTTCCGCCACTGCATTGTTCGCGGCTCGGCGGTGGTTTATCAAAAACCAAAAAAACCAAAAAAGTATTCAAATTATCTTTCCCCGTAGAACGATTCTCTACCGATACTTAGGGTTTTCAATTTGGTAACTTATATTCAATCTTCCAGAAAAACTAACCACCGATGGCGCAAGTTCACGAGAGTTCCAACAATGACGCTGCTGCTCCGGTTCCGAATCATCTCACAACGACGTCGTTGTATGTCGGAGATCTCGATCATGACGTCAATGATTCGCAGATTTACGATCTTTTCAATCAGATCGGTCAAGTTGTTTCTGTTCGGATCTGTAGGGATTTGGTTACTCAGCAATCCCTAGGTTACGGTTATGTCAATTTCAGTAACCCTCATGACGCTGCAAAGGCTATGGATGTGCTGAATTTCACTCCACTGAACAACAAACCTATTCGGATAATGTATTCTCACCGGGATCCTAGTGTTCGCAAGAGCGGTGCTGCCAATATTTTCATCAAGAATTTGGATAAGGCAATCGATCACAAGGGTTTATATGATACTTTTTCGGTTTTTGGAAGTATTCTCTCTTGCAAGATAGCAATGGATGCTTCTGGCCTGTCTAAAGGCTATGGTTTTGTGCAATATGAGAATGAAGAATCTGCACAAAGTGCAATTGATAAGTTAAACGGCATGCTGATCAATGATAAGCCTGTCTATGTTGGTCATTTCCAACGGAAGCAGGATAGAGATAACACCTTGAGTACTGCAAAGTTTAATAATGTCTATGTGAAAAATCTATCAGAGGCAATTGCTGATGATGACTTGAAGAGCATATTTGGAGAATATGGAACTATTACTAGTGCTGTAGTAATGAGAGATGTAGATGGTAAATCAAAATGTTTTGGTTTTGTCAATTTTGAAAATGCAGATGCTGCTGCTAACGCTGTGGAGGCACTTAATGGAAAGAAATTTGATGACAAGGAGTGGTATGTTGGAAAAGCCCTGAAAAAGTATGAAAGGGAGCTTGAACTGAAAGAAAGACATGAGCAGAGTATAAAGGAAACTGTTGACAAATATTATGGTGCCAACTTGTATCTCAAAAACTTGGATGATAGTGTCAGCGATGAAAAACTTAGCGAACTGTTCTCCGAGTTTGGTACAGTAACTTCATGCAAGATTCTGCGAGATCCACAGGGAATCAGTAGGGGATCTGGATTTGTTGCCTTTTCAACTCCTGAGGAAGCAACTCGAGCTCTTGCTGAGATGAATGGAAAAATGGTTGCTGGCAAACCTCTCTATGTTGCTCCTGCACAGAAAAAAGAAGAGAGAAAAGCAAGGTTACAGGCACAATTTTCGCAAGTGAGGCCGGTTGCAATGGCTCCTATTGCGCCGCGAGTGCCATTTTACCCTGCGGGAGTTCCAGGGATGGGACAGCAAATCATGTATGGCCAAGCTCCTCCAGCCTTCATATCTCAGGCTGGATTTGGTTATCCACAGCAACTGGTTCCTGGAATGAGACCAGGTGGTGCTCCAATGCCAAACTTCTTTGTGCCCATGGGTCAGCAGGGTCAACAAGGTCAGCGTCCTGGCGGACGGCGAGGTGCAGGTCCTATGCATCAAATCCCACAGTCCTTGCCAATGATGCAGCAACAGATGCTTCCAAGGGGACGTGTCTACCGATACCCCCCTCCAGGTCGCAACCTGCAAGGTGTTCCGCTGCCAGGAGTAACTGGAGGAACACCCATACAGGCGCTGGCTACAGCCCTTGCAAATGCTCCTCCTGAACAGCAGAGGACCATGCTTGGTGAAGTTTTATACCCCCTAGTAGAAAAGATTGAACATGGTGGAGCTGCAAAGGTTACTGGGATGCTTTTGGAAATGGACCAGCCTGAGGTGTTACATCTGATTGAGTCACCAGAGGCTCTCAAGACTAAAGTTGCTGAGGCCGTGGAAGTGCTGAGAAATGTTGCACAGCAGCAATCAAACAGCCCAGCTGATCAACTAGCTTCTCTCTCTCTTAATGACAATCACGAGTCTTAGACTCTGCATGTCACAGTTTACAGGGATCCATACGTTCTTTGAACGTAACAGTTTTGTGTTTCATGTCTTTTTGNTAGGATTAGCAATATTCCTTGGTATTTTCCTTGGATTAAGCTAGCATATTGATTTGTGGTTTGTTTTGTTGTCTTTTCACCGTNTTTGGTTNTTTGNTTGACTTNTTGACATTGTTNTA
TARGET=2091,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3002
PRIMER_LEFT_PENALTY=0.058624
PRIMER_RIGHT_PENALTY=0.241544
PRIMER_LEFT_SEQUENCE=GTCACCAGAGGCTCTCAAGACT
PRIMER_RIGHT_SEQUENCE=CAAAGAACGTATGGATCCCTGT
PRIMER_LEFT=1993,22
PRIMER_RIGHT=2163,22
PRIMER_LEFT_TM=60.059
PRIMER_RIGHT_TM=60.242
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=0.3146
PRIMER_LEFT_1_PENALTY=0.073038
PRIMER_RIGHT_1_PENALTY=0.241544
PRIMER_LEFT_1_SEQUENCE=GAAGTGCTGAGAAATGTTGCAC
PRIMER_RIGHT_1_SEQUENCE=CAAAGAACGTATGGATCCCTGT
PRIMER_LEFT_1=2033,22
PRIMER_RIGHT_1=2163,22
PRIMER_LEFT_1_TM=59.927
PRIMER_RIGHT_1_TM=60.242
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.4614
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=GTGGAAGTGCTGAGAAATGTTG
PRIMER_RIGHT_2_SEQUENCE=CAAAGAACGTATGGATCCCTGT
PRIMER_LEFT_2=2030,22
PRIMER_RIGHT_2=2163,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=0.4934
PRIMER_LEFT_3_PENALTY=0.058624
PRIMER_RIGHT_3_PENALTY=0.434743
PRIMER_LEFT_3_SEQUENCE=GTCACCAGAGGCTCTCAAGACT
PRIMER_RIGHT_3_SEQUENCE=CCAAANACGGTGAAAAGACAAC
PRIMER_LEFT_3=1993,22
PRIMER_RIGHT_3=2285,22
PRIMER_LEFT_3_TM=60.059
PRIMER_RIGHT_3_TM=60.435
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.5078
PRIMER_LEFT_4_PENALTY=0.073038
PRIMER_RIGHT_4_PENALTY=0.434743
PRIMER_LEFT_4_SEQUENCE=GAAGTGCTGAGAAATGTTGCAC
PRIMER_RIGHT_4_SEQUENCE=CCAAANACGGTGAAAAGACAAC
PRIMER_LEFT_4=2033,22
PRIMER_RIGHT_4=2285,22
PRIMER_LEFT_4_TM=59.927
PRIMER_RIGHT_4_TM=60.435
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_04520 Ps_contig_mira-and-tgicl-ass_franssen_33532
SEQUENCE=TACNCGGGGGCGGTTAAGATTTGGTATTTAAATAAATCGAGCGAATTGATGAAGGGACGACACGATACCTTTGCCCGTTACTGGTTGCGTTTTATTCACGCGTTTTCTTCTTCTTCCATTTATTACCCTTTCTTTCTCTGCATCTTGATTTGAATTGAATCAGAAAATGTCTACCCTTGTTCTACCACCTGTTCCTCCTTCCCCCAAAGACGATGCCACGGAACTTCACCGTGCTTTCAAAGGTTTTGGATGTGATACTGGCGCTGTTATCAATATACTTGCTCATAGAGACGCTGCTCAGCGCGCCTATCTCCAACAAGAATACCGAGCCATCTATTATGAGGATCTTATCAAACGATTATCTTCCGAGCTTTCTGGCAAATTGGAAAATGCTGTTCTTCTTTGGATGCATGACCCTGCCGGACGCGATGCTGTTATCATCAGGCAGTCTCTAACCGTCACCAAAATTCTTGATGCTGCCACTGAAGTTATTTGTTCCAGAACTCCAGCCCAGCTTCAATATTTAAGGCAGATTTACCATGCTAAATTCGGCGATTTTATTGATCATGATATTGAAAGGAATACCTCCGGGGATCATAAAAAGATTTTACTTGCATATCTAAACACACCACGCCACGAAGGCCCCGAGACTAATAGAGACATGGCTGAGAACGATGCGGTGGCTCTCTATAGAGCAGGTGAGAAGAAACTCGGAACGGATGAGAAAATTTTTGTGCAAATATTCAGTGGACACAGCGCCGCACAGTTGGCTGCCATAAATCAGTGTTACAATACCAAGTATGGACATTCGTTGAAAAAGGCAATAAAGAATGAAACATCCGGGCATTTTGCTCATGCACTTTCGACAATAGTCCAATGTGCCGAGAATCCTGCTAGGTATTTCGCAAAGGTCCTACGTAAGTCAATGAAAGGGTTGGGGACGGATGACACAAAGCTTATCAGGGTGATTGTTACAAGGTGTGAGATTGATCTACATTATATCAAAGCTGAATATTTAAAGAAATACAAGAAGACACTAAATGATGCAGTTCACTCCGAAACATCCGGTCAATACAGGACTTTTCTTCTCACACTTTTAGGTTCAAACCAGTAGTCCTCAAAATACATGTTTTCTGTGGAATAATTTCAACTATGATGTTGAATATAGGATACTTCCAATCTTTTTTTTCTTCATATTGTAAATTAAGCAGTGTTTGGTGAATTTATTTGCTTCTAAGTTGCATCATAACGCACAAATTGCCGGGCATTTTGTATATTTGTATGTACCTATGGATGGGTATGTATTAAGTATGTATGTATGTATGTAAGTGTTGGATATTTATTTTTGTTTTCCCTTGCCAAACATTGGTAATTTGATTTCTCTTGCTANNTTTTTTACTTTT
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0859
PRIMER_LEFT_PENALTY=0.054756
PRIMER_RIGHT_PENALTY=0.031122
PRIMER_LEFT_SEQUENCE=TACTGGTTGCGTTTTATTCACG
PRIMER_RIGHT_SEQUENCE=AGGAGGAACAGGTGGTAGAACA
PRIMER_LEFT=78,22
PRIMER_RIGHT=198,22
PRIMER_LEFT_TM=60.055
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.1033
PRIMER_LEFT_1_PENALTY=0.054756
PRIMER_RIGHT_1_PENALTY=0.048570
PRIMER_LEFT_1_SEQUENCE=TACTGGTTGCGTTTTATTCACG
PRIMER_RIGHT_1_SEQUENCE=TCCTCATAATAGATGGCTCGGT
PRIMER_LEFT_1=78,22
PRIMER_RIGHT_1=344,22
PRIMER_LEFT_1_TM=60.055
PRIMER_RIGHT_1_TM=59.951
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.2360
PRIMER_LEFT_2_PENALTY=0.054756
PRIMER_RIGHT_2_PENALTY=0.181270
PRIMER_LEFT_2_SEQUENCE=TACTGGTTGCGTTTTATTCACG
PRIMER_RIGHT_2_SEQUENCE=GCAGCGTCTCTATGAGCAAGTA
PRIMER_LEFT_2=78,22
PRIMER_RIGHT_2=296,22
PRIMER_LEFT_2_TM=60.055
PRIMER_RIGHT_2_TM=59.819
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.2971
PRIMER_LEFT_3_PENALTY=0.054756
PRIMER_RIGHT_3_PENALTY=0.242357
PRIMER_LEFT_3_SEQUENCE=TACTGGTTGCGTTTTATTCACG
PRIMER_RIGHT_3_SEQUENCE=AAGCTCGGAAGATAATCGTTTG
PRIMER_LEFT_3=78,22
PRIMER_RIGHT_3=372,22
PRIMER_LEFT_3_TM=60.055
PRIMER_RIGHT_3_TM=59.758
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=0.3038
PRIMER_LEFT_4_PENALTY=0.054756
PRIMER_RIGHT_4_PENALTY=0.249074
PRIMER_LEFT_4_SEQUENCE=TACTGGTTGCGTTTTATTCACG
PRIMER_RIGHT_4_SEQUENCE=GCCAGTATCACATCCAAAACCT
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=261,22
PRIMER_LEFT_4_TM=60.055
PRIMER_RIGHT_4_TM=60.249
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_04521 Ps_contig_mira-and-tgicl-ass_franssen_33532
SEQUENCE=TACNCGGGGGCGGTTAAGATTTGGTATTTAAATAAATCGAGCGAATTGATGAAGGGACGACACGATACCTTTGCCCGTTACTGGTTGCGTTTTATTCACGCGTTTTCTTCTTCTTCCATTTATTACCCTTTCTTTCTCTGCATCTTGATTTGAATTGAATCAGAAAATGTCTACCCTTGTTCTACCACCTGTTCCTCCTTCCCCCAAAGACGATGCCACGGAACTTCACCGTGCTTTCAAAGGTTTTGGATGTGATACTGGCGCTGTTATCAATATACTTGCTCATAGAGACGCTGCTCAGCGCGCCTATCTCCAACAAGAATACCGAGCCATCTATTATGAGGATCTTATCAAACGATTATCTTCCGAGCTTTCTGGCAAATTGGAAAATGCTGTTCTTCTTTGGATGCATGACCCTGCCGGACGCGATGCTGTTATCATCAGGCAGTCTCTAACCGTCACCAAAATTCTTGATGCTGCCACTGAAGTTATTTGTTCCAGAACTCCAGCCCAGCTTCAATATTTAAGGCAGATTTACCATGCTAAATTCGGCGATTTTATTGATCATGATATTGAAAGGAATACCTCCGGGGATCATAAAAAGATTTTACTTGCATATCTAAACACACCACGCCACGAAGGCCCCGAGACTAATAGAGACATGGCTGAGAACGATGCGGTGGCTCTCTATAGAGCAGGTGAGAAGAAACTCGGAACGGATGAGAAAATTTTTGTGCAAATATTCAGTGGACACAGCGCCGCACAGTTGGCTGCCATAAATCAGTGTTACAATACCAAGTATGGACATTCGTTGAAAAAGGCAATAAAGAATGAAACATCCGGGCATTTTGCTCATGCACTTTCGACAATAGTCCAATGTGCCGAGAATCCTGCTAGGTATTTCGCAAAGGTCCTACGTAAGTCAATGAAAGGGTTGGGGACGGATGACACAAAGCTTATCAGGGTGATTGTTACAAGGTGTGAGATTGATCTACATTATATCAAAGCTGAATATTTAAAGAAATACAAGAAGACACTAAATGATGCAGTTCACTCCGAAACATCCGGTCAATACAGGACTTTTCTTCTCACACTTTTAGGTTCAAACCAGTAGTCCTCAAAATACATGTTTTCTGTGGAATAATTTCAACTATGATGTTGAATATAGGATACTTCCAATCTTTTTTTTCTTCATATTGTAAATTAAGCAGTGTTTGGTGAATTTATTTGCTTCTAAGTTGCATCATAACGCACAAATTGCCGGGCATTTTGTATATTTGTATGTACCTATGGATGGGTATGTATTAAGTATGTATGTATGTATGTAAGTGTTGGATATTTATTTTTGTTTTCCCTTGCCAAACATTGGTAATTTGATTTCTCTTGCTANNTTTTTTACTTTT
TARGET=1309,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5254
PRIMER_LEFT_PENALTY=0.478666
PRIMER_RIGHT_PENALTY=2.046772
PRIMER_LEFT_SEQUENCE=CCGGTCAATACAGGACTTTTCT
PRIMER_RIGHT_SEQUENCE=GTTTGGCAAGGGAAAACAAA
PRIMER_LEFT=1064,22
PRIMER_RIGHT=1363,20
PRIMER_LEFT_TM=59.521
PRIMER_RIGHT_TM=59.953
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=3.3918
PRIMER_LEFT_1_PENALTY=1.448465
PRIMER_RIGHT_1_PENALTY=1.943362
PRIMER_LEFT_1_SEQUENCE=GGTTCAAACCAGTAGTCCTCAAA
PRIMER_RIGHT_1_SEQUENCE=ATTACCAATGTTTGGCAAGGG
PRIMER_LEFT_1=1099,23
PRIMER_RIGHT_1=1372,21
PRIMER_LEFT_1_TM=59.552
PRIMER_RIGHT_1_TM=60.943
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=274
PRIMER_PAIR_PENALTY_2=3.4952
PRIMER_LEFT_2_PENALTY=1.448465
PRIMER_RIGHT_2_PENALTY=2.046772
PRIMER_LEFT_2_SEQUENCE=GGTTCAAACCAGTAGTCCTCAAA
PRIMER_RIGHT_2_SEQUENCE=GTTTGGCAAGGGAAAACAAA
PRIMER_LEFT_2=1099,23
PRIMER_RIGHT_2=1363,20
PRIMER_LEFT_2_TM=59.552
PRIMER_RIGHT_2_TM=59.953
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=3.5283
PRIMER_LEFT_3_PENALTY=1.448465
PRIMER_RIGHT_3_PENALTY=2.079861
PRIMER_LEFT_3_SEQUENCE=GGTTCAAACCAGTAGTCCTCAAA
PRIMER_RIGHT_3_SEQUENCE=AATTACCAATGTTTGGCAAGGG
PRIMER_LEFT_3=1099,23
PRIMER_RIGHT_3=1373,22
PRIMER_LEFT_3_TM=59.552
PRIMER_RIGHT_3_TM=62.080
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=3.7168
PRIMER_LEFT_4_PENALTY=1.773411
PRIMER_RIGHT_4_PENALTY=1.943362
PRIMER_LEFT_4_SEQUENCE=GGTTCAAACCAGTAGTCCTCAA
PRIMER_RIGHT_4_SEQUENCE=ATTACCAATGTTTGGCAAGGG
PRIMER_LEFT_4=1099,22
PRIMER_RIGHT_4=1372,21
PRIMER_LEFT_4_TM=58.227
PRIMER_RIGHT_4_TM=60.943
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_04522 Ps_contig_mira-and-tgicl-ass_franssen_33537
SEQUENCE=TCGTCGGGGGCATTGTTTCTCTTTCTCCTTTTCTCTCCCTAGACCAAACCAAAACATAATAAACACAAAACAAAAACCTATTCGGTAATTCATTCCCAACCTTAAAACCTCCAATTTCATGTCTCGGATCCTGTAATTCGGATCTTCAAAACCCGACAAACCTCGTTCTCCTTCGATCCGGTTCAGAAAACCATGACGCGACGTTGTTCCCATTGCAGCCACAATGGGCACAACTCAAGAACGTGTCCCAACCGTGGCGTTAAGCTTTTCGGAGTTCGATTAACCGATGGGATCCGAAAGAGTGCTAGTATGGGTAATCTTAGTCATTATACTGGTTCCGGGTCTGGATCCCTTCATACCGGGTTAAATAATCTCGATTCACCCGGTGAAACACCTGATCACGCCGCCGTTGCTGACGGCTACGCCTCTGAGGATTTTGTTCCTGGCTCTTCTTCTACTTCCCGTGAAAGAAAAAAGGGTACTCCCTGGACTGAGGAGGAGCATAGAATGTTCTTACTTGGATTGCAGAAGCTGGGCAAAGGTGACTGGCGTGGAATTGCTAGGAACTATGTTATATCAAGGACACCTACTCAAGTGGCCAGTCATGCTCAGAAATATTTCATCAGGCAAAGCAACGTGTCTAGGAGGAAAAGACGGTCCAGCTTATTCGATATTGTTCAAGATGATGCTGCTGACACTTCAATGGTACCACAAGACTTCATGTCAGCTAATCCGCTACAGACTGAAACAGAAGGCAATAACCCTTTGCCTGCTCCTCCCCCACTCGATGAAGAATGCGAATCCATGGATTCCACAAACTCAAATGACGGAGAGTCTGCCTCTATCCCATTATTAAAGCCTGATAGCAATGCACAAACAGCGTCATATCCGGTAGTATATCCGGCCTATTATTCTCCATTCTTCCCATTTCCTCTTCCCTATTGGTCTGGATACAGTCCGGAGCCCGCTCCTAAGAACGAGACACATGAAGTGGTGAAGCCAACCGCAGTACATTCCAAGGCCCCGATCAATGTCGATGAACTGGTTGGCATGTCAAAACTGAGTTTAGGAGAGACTATTGGTGATGCCGGCCCCTCGACTCTGTCTCGTAAACTACTCGAAGAAGGACCTTCTAGACAGTCGGCTTTTCATGCAACACCAGCTTGTGGAACTGTTCTGTGAGCATTATTATCATGTGGCCTTGTGTAATTCATCCAATACATGTGGTTGTGAAATAACCTTAATAATTTTTATTTGCCCTTTCAGAATGGTTAACCTAGTTTGTTTGTTTGCTTGTAGACAGTAGCATCTAGGAATTACTTGGGTTTAAATTAAAAACTTGATGTTTGTTTAGTAGTTTTCATTTCTCAGGGTTATGTAAAATTGTGCAGTTTGACAATCTACCAAGGTTTGCTAAANNNTAAAATTTTGTCCTGTGGAACAAGAATTTTAAAAATCCCAGGAAGTTGAAACT
TARGET=1280,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2795
PRIMER_LEFT_PENALTY=0.046801
PRIMER_RIGHT_PENALTY=1.232712
PRIMER_LEFT_SEQUENCE=CTTGTGGAACTGTTCTGTGAGC
PRIMER_RIGHT_SEQUENCE=AGCAAACCTTGGTAGATTGTCA
PRIMER_LEFT=1162,22
PRIMER_RIGHT=1414,22
PRIMER_LEFT_TM=59.953
PRIMER_RIGHT_TM=58.767
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=1.2795
PRIMER_LEFT_1_PENALTY=0.046801
PRIMER_RIGHT_1_PENALTY=1.232712
PRIMER_LEFT_1_SEQUENCE=AGCTTGTGGAACTGTTCTGTGA
PRIMER_RIGHT_1_SEQUENCE=AGCAAACCTTGGTAGATTGTCA
PRIMER_LEFT_1=1160,22
PRIMER_RIGHT_1=1414,22
PRIMER_LEFT_1_TM=59.953
PRIMER_RIGHT_1_TM=58.767
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=1.2795
PRIMER_LEFT_2_PENALTY=0.046801
PRIMER_RIGHT_2_PENALTY=1.232712
PRIMER_LEFT_2_SEQUENCE=GCTTGTGGAACTGTTCTGTGAG
PRIMER_RIGHT_2_SEQUENCE=AGCAAACCTTGGTAGATTGTCA
PRIMER_LEFT_2=1161,22
PRIMER_RIGHT_2=1414,22
PRIMER_LEFT_2_TM=59.953
PRIMER_RIGHT_2_TM=58.767
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=254
PRIMER_PAIR_PENALTY_3=1.3145
PRIMER_LEFT_3_PENALTY=0.081801
PRIMER_RIGHT_3_PENALTY=1.232712
PRIMER_LEFT_3_SEQUENCE=GGCCTTGTGTAATTCATCCAAT
PRIMER_RIGHT_3_SEQUENCE=AGCAAACCTTGGTAGATTGTCA
PRIMER_LEFT_3=1197,22
PRIMER_RIGHT_3=1414,22
PRIMER_LEFT_3_TM=60.082
PRIMER_RIGHT_3_TM=58.767
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=1.4803
PRIMER_LEFT_4_PENALTY=0.247631
PRIMER_RIGHT_4_PENALTY=1.232712
PRIMER_LEFT_4_SEQUENCE=CATGTGGCCTTGTGTAATTCAT
PRIMER_RIGHT_4_SEQUENCE=AGCAAACCTTGGTAGATTGTCA
PRIMER_LEFT_4=1192,22
PRIMER_RIGHT_4=1414,22
PRIMER_LEFT_4_TM=59.752
PRIMER_RIGHT_4_TM=58.767
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_04523 Ps_contig_mira-and-tgicl-ass_franssen_33542
SEQUENCE=TTTTAACTTCTAGTTTTTTTGTTCTTGTGTGGAAATGACATTGTGAATTCTATCGAATTGAACTCCTCTATGTAATCTTTTCATCTATAGTCCGGCAGATATATATATTCAATATGAAATTAATTGG
TARGET=99,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04524 Ps_contig_mira-and-tgicl-ass_franssen_33544
SEQUENCE=CCCCCGGGGTTTAAACCCCCGTTTTTAAAACCCGGTTTAACCCCCGTAAACCCCCTTAACCCCCTTTTCCCCCAACCTTTTTTTTTAACCCCTTTTTTTCCCCCCCCTTTTAAAAAACCCCGGTAAACCCGTTTTTACCCCCTAACCTTTTTTTACCTTTAACCCCTTTACCCGTCCAAACGGGACCCGAAACTTACTACCCAACTGTCCGACTGACGAGAGAGAGAGAAGGAAGGTGGAGTCTCACCTCCTCCCTTCGATGTTGTTAACCAAGTACAAAAGACGCCGCAGAGATCGCTAACAAGGCTCTGCAGTTGGTTATTTCAGAATGTAAACCTAAGGTCAAGATTGTGGACATTTGCGAGAAAGGGGATTCGTTCATTAGAGAGCAAACTGGTAACATGTACAAGAATGTCAAGAAGAAGATTGAGAGAGGTGTTGCCTTCCCAACTTGCATTTCTGTAAACAATACTGTCTGTCATTTCTCTCCTCTTGCTAGTGATGAAACGGTTTTGGAAGAAGGGGATATATTGAAAATTGATTTGGCATGTCATATTGATGGGTTTATTGCTGCTGTGGCTCACACACATGTTCTTCAAGAGGGGCCTGTTACAGGACGTGCAGCGGATGTCATTGCTGCAGCAAACACGGCTGCTGAAGTGGCATTGAGGCTTGTTAGGCCTGGAAAGAAGAACCAAGATGTAACTGATGCGATTCAGAAGGTTGCTGCTGCCTATGATTGTAGGATTGTTGAAGGTGTTCTTAGCCACCAAATGAAGCAGTTTGTGATTGATGCAAATAAGGTTGTGCTAAATGTATCTAATCCTGATACAAGGGTTGATGATGCGGAGTTTGAAGAGAATGAAGTTTATGCAATTGATATTGTAGCCAGCACCGGAGAAGGCAAGCCTAAGCTGTTAGATGAGAAACAGACAACTATTTATAAGAGAGCTGTTGATAGAAGTTATAACTTGAAGATGAAAGCATCTAGGTTCATTTACAGTGAAATTAGCCAAAATTTTCCCATCATGCCATTCTCTGCCAGGGCTTTGGAAGAAAAAAGAGCTCGTCTGGGTTTAGTGGAATGCATGAATCATGAGCTTCTGCAACCATATCCAGTTCTACATGAAAAATCTGGTGATTATGTTGCCCATATCAAATTCACAGTCTTGCTTATGCCAAATGGTTCAGATCGTATCACATCTCACTCACTCCAGGAAATTCAGCCTACTAAAACAGTAGATGATCCTGAGATTAAAGCCTGGTTAGCCTTGGGTGTTAAGACAAAGAAGAAGGGCGGTGGGAAGAAGAAGAAAGGTAAGAAAGTTACTGAAGGGGAAGCCGAAGCTGATCCCATGGATACAACAAACGGTGCCGGGGCTGCAACACAAGAGCAAAATTGAGGCTGCATAAAAATCCTTAGGATTTCACTTTGGTCCCTTTCAATTTTGTTACATTTTTCCCTTAGCATTCCGACAATGTCAATAGGAATTTCCATGTGCACATTCCAGTACTACCTTGACAAGAATGCTATCTGTCTGTTTAAAACATTACTTGTGCTGTGTAGTTTTATTTGAAGCAAAGTAAATCAAATTTAAGAACAAGACTTTATATCTGTAAGTGTGCGCACCTGTTGAAGTAGGTCTTAAACTTGGTCGTGGTTGAATTTTTCAAATACCTAGGCATATGCGGAGATTGTGTTTCCACAAACGTTCACTCTTTACCAAATAGTCCATATTAAACTCTTAGCGTCACACTCATCAATTTGCTACTCCCTCTGTCTAATAAAAATTGTCTCTTTTCAATGTCACAAAATATCTGTCCTTTTACAATA
TARGET=218,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1937
PRIMER_LEFT_PENALTY=0.001341
PRIMER_RIGHT_PENALTY=0.192310
PRIMER_LEFT_SEQUENCE=TACCTTTAACCCCTTTACCCGT
PRIMER_RIGHT_SEQUENCE=ATAACCAACTGCAGAGCCTTGT
PRIMER_LEFT=153,22
PRIMER_RIGHT=321,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.192
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.1000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=0.2122
PRIMER_LEFT_1_PENALTY=0.001341
PRIMER_RIGHT_1_PENALTY=0.210886
PRIMER_LEFT_1_SEQUENCE=TACCTTTAACCCCTTTACCCGT
PRIMER_RIGHT_1_SEQUENCE=GAAATAACCAACTGCAGAGCCT
PRIMER_LEFT_1=153,22
PRIMER_RIGHT_1=324,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.789
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.2559
PRIMER_LEFT_2_PENALTY=0.001341
PRIMER_RIGHT_2_PENALTY=0.254537
PRIMER_LEFT_2_SEQUENCE=TACCTTTAACCCCTTTACCCGT
PRIMER_RIGHT_2_SEQUENCE=GGCAACACCTCTCTCAATCTTC
PRIMER_LEFT_2=153,22
PRIMER_RIGHT_2=442,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.255
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.2559
PRIMER_LEFT_3_PENALTY=0.001341
PRIMER_RIGHT_3_PENALTY=0.254537
PRIMER_LEFT_3_SEQUENCE=TACCTTTAACCCCTTTACCCGT
PRIMER_RIGHT_3_SEQUENCE=GAAGGCAACACCTCTCTCAATC
PRIMER_LEFT_3=153,22
PRIMER_RIGHT_3=445,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.255
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.2710
PRIMER_LEFT_4_PENALTY=0.078668
PRIMER_RIGHT_4_PENALTY=0.192310
PRIMER_LEFT_4_SEQUENCE=CCCGAAACTTACTACCCAACTG
PRIMER_RIGHT_4_SEQUENCE=ATAACCAACTGCAGAGCCTTGT
PRIMER_LEFT_4=185,22
PRIMER_RIGHT_4=321,22
PRIMER_LEFT_4_TM=59.921
PRIMER_RIGHT_4_TM=60.192
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_04525 Ps_contig_mira-and-tgicl-ass_franssen_33544
SEQUENCE=CCCCCGGGGTTTAAACCCCCGTTTTTAAAACCCGGTTTAACCCCCGTAAACCCCCTTAACCCCCTTTTCCCCCAACCTTTTTTTTTAACCCCTTTTTTTCCCCCCCCTTTTAAAAAACCCCGGTAAACCCGTTTTTACCCCCTAACCTTTTTTTACCTTTAACCCCTTTACCCGTCCAAACGGGACCCGAAACTTACTACCCAACTGTCCGACTGACGAGAGAGAGAGAAGGAAGGTGGAGTCTCACCTCCTCCCTTCGATGTTGTTAACCAAGTACAAAAGACGCCGCAGAGATCGCTAACAAGGCTCTGCAGTTGGTTATTTCAGAATGTAAACCTAAGGTCAAGATTGTGGACATTTGCGAGAAAGGGGATTCGTTCATTAGAGAGCAAACTGGTAACATGTACAAGAATGTCAAGAAGAAGATTGAGAGAGGTGTTGCCTTCCCAACTTGCATTTCTGTAAACAATACTGTCTGTCATTTCTCTCCTCTTGCTAGTGATGAAACGGTTTTGGAAGAAGGGGATATATTGAAAATTGATTTGGCATGTCATATTGATGGGTTTATTGCTGCTGTGGCTCACACACATGTTCTTCAAGAGGGGCCTGTTACAGGACGTGCAGCGGATGTCATTGCTGCAGCAAACACGGCTGCTGAAGTGGCATTGAGGCTTGTTAGGCCTGGAAAGAAGAACCAAGATGTAACTGATGCGATTCAGAAGGTTGCTGCTGCCTATGATTGTAGGATTGTTGAAGGTGTTCTTAGCCACCAAATGAAGCAGTTTGTGATTGATGCAAATAAGGTTGTGCTAAATGTATCTAATCCTGATACAAGGGTTGATGATGCGGAGTTTGAAGAGAATGAAGTTTATGCAATTGATATTGTAGCCAGCACCGGAGAAGGCAAGCCTAAGCTGTTAGATGAGAAACAGACAACTATTTATAAGAGAGCTGTTGATAGAAGTTATAACTTGAAGATGAAAGCATCTAGGTTCATTTACAGTGAAATTAGCCAAAATTTTCCCATCATGCCATTCTCTGCCAGGGCTTTGGAAGAAAAAAGAGCTCGTCTGGGTTTAGTGGAATGCATGAATCATGAGCTTCTGCAACCATATCCAGTTCTACATGAAAAATCTGGTGATTATGTTGCCCATATCAAATTCACAGTCTTGCTTATGCCAAATGGTTCAGATCGTATCACATCTCACTCACTCCAGGAAATTCAGCCTACTAAAACAGTAGATGATCCTGAGATTAAAGCCTGGTTAGCCTTGGGTGTTAAGACAAAGAAGAAGGGCGGTGGGAAGAAGAAGAAAGGTAAGAAAGTTACTGAAGGGGAAGCCGAAGCTGATCCCATGGATACAACAAACGGTGCCGGGGCTGCAACACAAGAGCAAAATTGAGGCTGCATAAAAATCCTTAGGATTTCACTTTGGTCCCTTTCAATTTTGTTACATTTTTCCCTTAGCATTCCGACAATGTCAATAGGAATTTCCATGTGCACATTCCAGTACTACCTTGACAAGAATGCTATCTGTCTGTTTAAAACATTACTTGTGCTGTGTAGTTTTATTTGAAGCAAAGTAAATCAAATTTAAGAACAAGACTTTATATCTGTAAGTGTGCGCACCTGTTGAAGTAGGTCTTAAACTTGGTCGTGGTTGAATTTTTCAAATACCTAGGCATATGCGGAGATTGTGTTTCCACAAACGTTCACTCTTTACCAAATAGTCCATATTAAACTCTTAGCGTCACACTCATCAATTTGCTACTCCCTCTGTCTAATAAAAATTGTCTCTTTTCAATGTCACAAAATATCTGTCCTTTTACAATA
TARGET=1304,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1016
PRIMER_LEFT_PENALTY=0.027715
PRIMER_RIGHT_PENALTY=0.073900
PRIMER_LEFT_SEQUENCE=AGTCTTGCTTATGCCAAATGGT
PRIMER_RIGHT_SEQUENCE=CTCAATTTTGCTCTTGTGTTGC
PRIMER_LEFT=1165,22
PRIMER_RIGHT=1403,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=59.926
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.1226
PRIMER_LEFT_1_PENALTY=0.048688
PRIMER_RIGHT_1_PENALTY=0.073900
PRIMER_LEFT_1_SEQUENCE=CTGGTTAGCCTTGGGTGTTAAG
PRIMER_RIGHT_1_SEQUENCE=CTCAATTTTGCTCTTGTGTTGC
PRIMER_LEFT_1=1261,22
PRIMER_RIGHT_1=1403,22
PRIMER_LEFT_1_TM=60.049
PRIMER_RIGHT_1_TM=59.926
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.1557
PRIMER_LEFT_2_PENALTY=0.081801
PRIMER_RIGHT_2_PENALTY=0.073900
PRIMER_LEFT_2_SEQUENCE=AAATCTGGTGATTATGTTGCCC
PRIMER_RIGHT_2_SEQUENCE=CTCAATTTTGCTCTTGTGTTGC
PRIMER_LEFT_2=1130,22
PRIMER_RIGHT_2=1403,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=59.926
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=0.1796
PRIMER_LEFT_3_PENALTY=0.048688
PRIMER_RIGHT_3_PENALTY=0.130883
PRIMER_LEFT_3_SEQUENCE=CTGGTTAGCCTTGGGTGTTAAG
PRIMER_RIGHT_3_SEQUENCE=TTGACATTGTCGGAATGCTAAG
PRIMER_LEFT_3=1261,22
PRIMER_RIGHT_3=1484,22
PRIMER_LEFT_3_TM=60.049
PRIMER_RIGHT_3_TM=60.131
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.2043
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.073900
PRIMER_LEFT_4_SEQUENCE=CACTCCAGGAAATTCAGCCTAC
PRIMER_RIGHT_4_SEQUENCE=CTCAATTTTGCTCTTGTGTTGC
PRIMER_LEFT_4=1209,22
PRIMER_RIGHT_4=1403,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.926
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_04526 Ps_contig_mira-and-tgicl-ass_franssen_33545
SEQUENCE=CCGTCTTTTTTTGTTTTGTTTTGGTCTTATGTTTYTCTTGGCTTTTGTCTGTCATTCTCATTCCRCAAATATATCATATCATTCTTTGCATGTAGTTATATCATATCATTCTTTACAAGCATGAATCAAATGCATAGATATATTATCTCATTCAGAAGTCATTAGCTCCTTCAGGAATGTAAAGAGAGAGAATTTGAAAATCCAATGATGTGTATTGTCTTCATTTTTTCTCCTCTGGTTTCTCTTCTTTTTTCTCTTCCTTTTCTTCTTTCTTCTCTTCTTTCTTCTCTTCTATGGCAGGTCCAACTGATACTATATCTACCTTCCCCACTTTCTTCAGCTTCTTCACTACTGCCACTGTATCCATTTCACCAATCACCGTTAACTTCTGTTCATTAACATCTGTCTCGATTGAATCAACTCCATAGATATCTGCAACTGCTTCTATTGATTTCTTCTTTGTTTTGTCATCTGTCATAGTAAGAACCTTTAACACCACCTTCTGCTATGGATCTGAGCCATTGTGAGAAGGGAAGAAGTGTGTGAAAACAAGGAAGAGGAGAATGAAGGCTTGAGAATA
TARGET=9,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04527 Ps_contig_mira-and-tgicl-ass_franssen_33547
SEQUENCE=GATCTAGGACATTAATTTAAACCCAAAAAGATGATAAGATTGTTATTGAAAACTCTATTTCTCGAGATATTGTTGCTTCTGCTTCATCATCATTCAAGTGCTGTGGATGATGATGATCTCTCTCCATTCCCTAAGAACTTTTTGTTTGGAACTGCTTCTTCTTCTTACCAGTATGAAGGTGGTTATAACATTGATGGCAAGGGACAGAGTAATTGGGATAACTTCACTCATGGAGGTAGAAGTATGATAGTGGATGGAAGTAATGGGGATATTGCAGTTGACCATTATCATCGTTTCCAGGAGGATATAGATCTATTGGAAGCTTTGAAAGTAAATAGTTACCGACTCTCCATATCATGGACAAGAATTCTACCAAAGGGTAGGTTTGGAGAAATCAATTGGCCTGGTATTGACTTCTATAACAAACTATTTGATGCCTTGCTGCTCAAAGGTATCCAACCCTTTGTCACTCTAAGTCACTATGACAGTCCACAAGAACTAGAGGACAGATATGGAGGATGGTTAAGTCCCCAATCACAGGAAGATTTTGCATTTTATGCAGACCTATGCTTCAAAACATTTGGTGACAGAGTTAAGTATTGGGTCACATTCAACGAACCAAATTACCAAGTACCACTCGGTTACCGATCAGGCATATATCCCCCGTCTCGTTGCTCTGGCTTATTGGCAATGGCTAACTGTAAAGAAGGTGATTCAGAAAAAGAGCCCTTATAGCAGCCCATAA
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4512
PRIMER_LEFT_PENALTY=0.360621
PRIMER_RIGHT_PENALTY=0.090580
PRIMER_LEFT_SEQUENCE=TGCTTCTGCTTCATCATCATTC
PRIMER_RIGHT_SEQUENCE=TATCCCCATTACTTCCATCCAC
PRIMER_LEFT=73,22
PRIMER_RIGHT=270,22
PRIMER_LEFT_TM=60.361
PRIMER_RIGHT_TM=59.909
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=0.4512
PRIMER_LEFT_1_PENALTY=0.360621
PRIMER_RIGHT_1_PENALTY=0.090580
PRIMER_LEFT_1_SEQUENCE=GCTTCTGCTTCATCATCATTCA
PRIMER_RIGHT_1_SEQUENCE=TATCCCCATTACTTCCATCCAC
PRIMER_LEFT_1=74,22
PRIMER_RIGHT_1=270,22
PRIMER_LEFT_1_TM=60.361
PRIMER_RIGHT_1_TM=59.909
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=0.4853
PRIMER_LEFT_2_PENALTY=0.394711
PRIMER_RIGHT_2_PENALTY=0.090580
PRIMER_LEFT_2_SEQUENCE=TTGTTGCTTCTGCTTCATCATC
PRIMER_RIGHT_2_SEQUENCE=TATCCCCATTACTTCCATCCAC
PRIMER_LEFT_2=69,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=60.395
PRIMER_RIGHT_2_TM=59.909
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.5340
PRIMER_LEFT_3_PENALTY=0.360621
PRIMER_RIGHT_3_PENALTY=0.173354
PRIMER_LEFT_3_SEQUENCE=TGCTTCTGCTTCATCATCATTC
PRIMER_RIGHT_3_SEQUENCE=CCTCCATGAGTGAAGTTATCCC
PRIMER_LEFT_3=73,22
PRIMER_RIGHT_3=235,22
PRIMER_LEFT_3_TM=60.361
PRIMER_RIGHT_3_TM=59.827
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=163
PRIMER_PAIR_PENALTY_4=0.5340
PRIMER_LEFT_4_PENALTY=0.360621
PRIMER_RIGHT_4_PENALTY=0.173354
PRIMER_LEFT_4_SEQUENCE=GCTTCTGCTTCATCATCATTCA
PRIMER_RIGHT_4_SEQUENCE=CCTCCATGAGTGAAGTTATCCC
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=60.361
PRIMER_RIGHT_4_TM=59.827
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_04528 Ps_contig_mira-and-tgicl-ass_franssen_33547
SEQUENCE=GATCTAGGACATTAATTTAAACCCAAAAAGATGATAAGATTGTTATTGAAAACTCTATTTCTCGAGATATTGTTGCTTCTGCTTCATCATCATTCAAGTGCTGTGGATGATGATGATCTCTCTCCATTCCCTAAGAACTTTTTGTTTGGAACTGCTTCTTCTTCTTACCAGTATGAAGGTGGTTATAACATTGATGGCAAGGGACAGAGTAATTGGGATAACTTCACTCATGGAGGTAGAAGTATGATAGTGGATGGAAGTAATGGGGATATTGCAGTTGACCATTATCATCGTTTCCAGGAGGATATAGATCTATTGGAAGCTTTGAAAGTAAATAGTTACCGACTCTCCATATCATGGACAAGAATTCTACCAAAGGGTAGGTTTGGAGAAATCAATTGGCCTGGTATTGACTTCTATAACAAACTATTTGATGCCTTGCTGCTCAAAGGTATCCAACCCTTTGTCACTCTAAGTCACTATGACAGTCCACAAGAACTAGAGGACAGATATGGAGGATGGTTAAGTCCCCAATCACAGGAAGATTTTGCATTTTATGCAGACCTATGCTTCAAAACATTTGGTGACAGAGTTAAGTATTGGGTCACATTCAACGAACCAAATTACCAAGTACCACTCGGTTACCGATCAGGCATATATCCCCCGTCTCGTTGCTCTGGCTTATTGGCAATGGCTAACTGTAAAGAAGGTGATTCAGAAAAAGAGCCCTTATAGCAGCCCATAA
TARGET=155,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1275
PRIMER_LEFT_PENALTY=0.036956
PRIMER_RIGHT_PENALTY=0.090580
PRIMER_LEFT_SEQUENCE=TGATGATCTCTCTCCATTCCCT
PRIMER_RIGHT_SEQUENCE=TATCCCCATTACTTCCATCCAC
PRIMER_LEFT=110,22
PRIMER_RIGHT=270,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.909
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=0.2103
PRIMER_LEFT_1_PENALTY=0.036956
PRIMER_RIGHT_1_PENALTY=0.173354
PRIMER_LEFT_1_SEQUENCE=TGATGATCTCTCTCCATTCCCT
PRIMER_RIGHT_1_SEQUENCE=CCTCCATGAGTGAAGTTATCCC
PRIMER_LEFT_1=110,22
PRIMER_RIGHT_1=235,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.827
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.2382
PRIMER_LEFT_2_PENALTY=0.036956
PRIMER_RIGHT_2_PENALTY=0.201288
PRIMER_LEFT_2_SEQUENCE=TGATGATCTCTCTCCATTCCCT
PRIMER_RIGHT_2_SEQUENCE=ATCCTCCTGGAAACGATGATAA
PRIMER_LEFT_2=110,22
PRIMER_RIGHT_2=305,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=59.799
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.3363
PRIMER_LEFT_3_PENALTY=0.036956
PRIMER_RIGHT_3_PENALTY=0.299343
PRIMER_LEFT_3_SEQUENCE=TGATGATCTCTCTCCATTCCCT
PRIMER_RIGHT_3_SEQUENCE=CTGCAATATCCCCATTACTTCC
PRIMER_LEFT_3=110,22
PRIMER_RIGHT_3=276,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=59.701
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=167
PRIMER_PAIR_PENALTY_4=0.4095
PRIMER_LEFT_4_PENALTY=0.036956
PRIMER_RIGHT_4_PENALTY=0.372557
PRIMER_LEFT_4_SEQUENCE=TGATGATCTCTCTCCATTCCCT
PRIMER_RIGHT_4_SEQUENCE=CAATTACTCTGTCCCTTGCCAT
PRIMER_LEFT_4=110,22
PRIMER_RIGHT_4=214,22
PRIMER_LEFT_4_TM=60.037
PRIMER_RIGHT_4_TM=60.373
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_04529 Ps_contig_mira-and-tgicl-ass_franssen_33553
SEQUENCE=GTTAGTCAATAATTTCATACATACTTATACAGTTGAGAGTGAAAGGAAAGAATACNAATAATTATCATTTCAATATGCCTCTGATGCACCATGCCCGTCTTCCAGACAAACCGAAGAAGATGACTCGAGATCAAATCGATGCTGGAAATAAAATAATGCAAATATTGATAGATTTGATGCTTCGAAGTTTCCGACGAGGTTTGCTGGTCAGATCCGGGACTTCAGCTCTGAGGGTTATATTGATGGGAAGAATGACCGGAGACTCGATGATTGTCTCCGGTACTGCATTGTTGCTGGGAAGAAAGCACTTGAGCATGCTGGCCTCGGCGGTGTAAATCTCTCTAAGATTGATAAGGAGCGTGCTGGTGTTTTGGTTGGGTCTGGGATGGGTGGTTTAACGGTGTTTTCTGAAGGGGTTAAGAATTTGATTGAGAAAGGACATAGGAAGATAACACCTTTTTTTATTCCTTATGCTATTACGAATATGGGTTCGGCTTTGCTTGGGATTGATTTGGGTTTCATGGGTCCAAACTATTCTATTTCAACTGCTTGTGCTACTTCGAATTATTGCTTTTATGCTGCTGCCAATCATATTCGTAGGGGTGAGGCGGATTTGATGATAGCAGGTGGGACTGAAGCTGCTATTATTCCTATTGGTTTGGGGGGTTTTGTTGCTTGCAGGGCACTTTCTCAGAGAAATGATGACCCTAGAACTGCTTCCAGGCCTTGGGATAAAGACCGTGATGGTTTTGTTATGGGTGAAGGTTCTGGAGTTCTGGTATTGGAAAGCCTTGAACATGCCATGAAGCGAGGTGCACCTATCATTGCTGAATACTTGGGAGGTGCTGTGAACTGTGATGCTCATCACATGACTGACCCAAGAGCCGATGGACTTGGTGTGTCTACATGCATCCAGAGCAGCCTTGTAGATGCTGGTGTGTCCCCTGAAGAGGTCAACTACATAAATGCACATGCAACTTCCACTCTTGCGGGTGACTTGGCAGAGATTAATGCTATCAAAAAGGTTTTCAAGAACACCTCTGGCATCAAAATTAATGCCACCAAGTCTATGATAGGGCACTGCCTTGGTGCAGCTGGTGGTTTGGAAGCTATTGCCACAGTGAAAGCCATAACAACAGGATGGTTGCATCCATCCATCAATCAATTCAACCCAGAACCTGCAGTTGATTTTGATACAGTGCCAAATGTTAAGCAGCAGCATGAAATTAATGTTGGTATTTCAAATTCATTTGGATTTGGTGGACACAACTCTGTTGTGGCATTTTCCGCTTTCAGGCCCTGATTTCCAAGCTTGGTTGCACATCATGTAGAACATGGAATCCAAATTAATTGAAATTTTGTTCTCTCTACATCATTGAGGCTATTTATTTAATGAGTTCATAATATATAAAAGCTGTAGTTCTGTAATTGTTAATTTTATGCTAATTGTGAACAGACACTTATAAAGACTATTTTGCTGTAAAACTTTTTAAATGTAGCACGGAGATTTCATTGGTTTGAAGTTATAAAGCGGATAGAAAGTGTTGAAAGAGCTT
TARGET=1148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2880
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.283676
PRIMER_LEFT_SEQUENCE=AGAACACCTCTGGCATCAAAAT
PRIMER_RIGHT_SEQUENCE=TCAAAATCAACTGCAGGTTCTG
PRIMER_LEFT=1031,22
PRIMER_RIGHT=1193,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.284
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.6681
PRIMER_LEFT_1_PENALTY=0.384450
PRIMER_RIGHT_1_PENALTY=0.283676
PRIMER_LEFT_1_SEQUENCE=CATAAATGCACATGCAACTTCC
PRIMER_RIGHT_1_SEQUENCE=TCAAAATCAACTGCAGGTTCTG
PRIMER_LEFT_1=960,22
PRIMER_RIGHT_1=1193,22
PRIMER_LEFT_1_TM=60.384
PRIMER_RIGHT_1_TM=60.284
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.7817
PRIMER_LEFT_2_PENALTY=0.498031
PRIMER_RIGHT_2_PENALTY=0.283676
PRIMER_LEFT_2_SEQUENCE=CCCCTGAAGAGGTCAACTACAT
PRIMER_RIGHT_2_SEQUENCE=TCAAAATCAACTGCAGGTTCTG
PRIMER_LEFT_2=941,22
PRIMER_RIGHT_2=1193,22
PRIMER_LEFT_2_TM=59.502
PRIMER_RIGHT_2_TM=60.284
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.8786
PRIMER_LEFT_3_PENALTY=0.003589
PRIMER_RIGHT_3_PENALTY=0.875033
PRIMER_LEFT_3_SEQUENCE=TGAAAGCCATAACAACAGGATG
PRIMER_RIGHT_3_SEQUENCE=GTTGTGTCCACCAAATCCAAAT
PRIMER_LEFT_3=1121,22
PRIMER_RIGHT_3=1269,22
PRIMER_LEFT_3_TM=59.996
PRIMER_RIGHT_3_TM=60.875
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=0.8794
PRIMER_LEFT_4_PENALTY=0.004359
PRIMER_RIGHT_4_PENALTY=0.875033
PRIMER_LEFT_4_SEQUENCE=AGAACACCTCTGGCATCAAAAT
PRIMER_RIGHT_4_SEQUENCE=GTTGTGTCCACCAAATCCAAAT
PRIMER_LEFT_4=1031,22
PRIMER_RIGHT_4=1269,22
PRIMER_LEFT_4_TM=60.004
PRIMER_RIGHT_4_TM=60.875
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_04530 Ps_contig_mira-and-tgicl-ass_franssen_33556
SEQUENCE=GCAGACTCAAGTATTATTTTAAAAATGATTTAAGGTTTCATACAACAACAACAACATCATCATCAAGGTTTCAATGTTCTCCTAGTGGTACTGTAGCACTTCTCAAACTAGTGTCCTTCACTAGAAAACCTTCACACTTGCATTTCTTAAGGCTTCATAGCCATTCTCGCGGTTTGGGATGGCAATGTCGACACCACAAAGGGGAACACCATTCTTCTCCCAATCCTTCACAGATATTAGAAATTGGGTTCAATAGGATGCTTGTTTCTCTCCATGATTGGAGAAATTTACGCATCATGGAACTAGCTGGCTTATTGCTTTGCATTTTAATGATTGTTCCGTCAGCTGATGCTGTCGATGCTCTCAAAACTTGTGCCTGTTTGCTCAAGGAATGCAGGCCAGAATTAGCCAAGTGTCTTTCAAACCCGTCATGTGCTGCCAATATTGCTTGTCTCCAAACTTGCAACAATAGACCTGATGAGACTGAATGCCAAATTAGATGCGGAGACGTGTTTGCAAACAGCGTGGTTGATGAATTCAACGAGTGCGCCGTCTCAAGGAAGAAATGTGTGCCTAAGAAATCTGATGTGGGAGACTTTCCCGTTCCAAACCCGGATGTCCTTGTCAAGAGCTTTAACATTTCAGATTTTAGTGGCAAGTGGTACATCACTAGTGGGTTAAATCCCACTTTTGACGCGTTTGATTGCCAGTTACATGAATTTCAAACAGAAGCAAATAAACTTGTTGGTAATATAACATGGAGAATACCAACTCCAGATGGTGGATTTCTCACAAGGTCAGCCCTGCAGAAATTTGTTCAAGATCCCAATAATCCAGGAATACTCTACAATCATGATAATGAGTACCTTAACTATCAAGATGACTGGTATATTTTGTCATCTCAAACTGAGAACAAACCAGACGACTATATATTCATATACTACCGAGGAAGAAACGATGCATGGGATGGCTATGGTGGTGCTGTTGTATACACAAGGAGTTCAGTTTTACCTGAATCCATTGTTCCTGAACTTGAGAAAGTGGCAAAGAGCGTAGGAAGAGACTTTAGCAATTTCATAAGAACAGATAACACATGTGGACCGGAGCCTTCCATTGTGGAGAGGCTGGAGAAAAAAGTAGAGGAAGGAGAGAAAACCATTGTGAGGGAAGTTGAAGAGTTGGAAGAAGAGGTAGAGAAGGTTGGTAAAACCGAAATGAACTTGTTACAGAAGTTGGCTGAAGGATTCAAAATATTTCAAGAAGATGAAGAAAATTTCTTAAGAGGGCTATCTAAGGAAGAAATGGAAATACTCAACAGCCTCAAATTGGAAGCTGATGAAGTAGAAAAGCTCTTTGGACGTGCTTTGCCACTGAGGAAACTAAGATAACTTATATAAATAAGTGAAAAAAATGATGTAAGTTGTAACATATTTTTTGATATACTTTGTTTGTTATTGAATGAATTCTCATGTTTGTTCTCATAAGAATTCGTTTTATATAGAAAAAAAGACATCAAATTGGTGCTGTTTTTTCTTTAAATTTTTTCTTTAGAAACGAGTCAGAAGCCCCAGAATTCTTCCAAACACTGATTCGG
TARGET=42,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04531 Ps_contig_mira-and-tgicl-ass_franssen_33568
SEQUENCE=TCAAGTGGAGGCAACGCATGGTGTTATGANTAGCAGTCGGCCAATTGTGCTTNAAGGGAGAGGCGATTGTTCTGCCCTTACTGTGATGACGGATTTGTTCAAGAACTTGATGAGATGCGAGGCGTTGCACCTCAAAACACCCTTCCGTCACGCTCGGGAGAGTTTCATCAAATGCCTGACTTATTTGATGCTATTCATGCTTTTGTGGGGAACAGAGGTTCCGAAAACAGGTTTGGGCTTATGGATGCTGTTGATAATTTCATGCGGCATAGGATGGCTGGAATGCACCCGAACTTTGATGTTAGAGGGAGGTCCGGTTCGTCTGTCCCGGTTCCTGAACAGAGTTGGGGCGTTTATAGTTCCGGTCCTTTTTTAGTATTTCACGGTCAAGTTCCTGGATTAAACTTGCCGAATGGAAGCTCGAGAGGAGGTTCCAGGCGCGGTGATTTTGGTGACTACTTTATGGGTTCTGGACTGGAAGAACTTATCGAACAACTCACCATGAATGATCGGCGTGGTCCAGCCCCAGCTGCACGATCATCAATTGATGCAATGCCGACTATTAGGATCACACAGGCGCATCTTCGCTCGGACTCACATTGTCCAGTCTGTAAAGAAAAATTCGAACTTGGTTCTGAAGCAAGAGAGATGCCATGCGACCATATTTACCATTCTGACTGTATTATTCCTTGGTTGGTTCAACATAACTCGTGTCCTGTTTGTCGCGTCGAGCTGCCACCACAAGGACAAGTTAGTTCTCGTGGTGGTGGTGGTGGTCGAAGTTGGGGAGGAAGGAATGGTGGCAACAACGGTGGAAATGGGAATAGCAGAGAGAGTGACCGTCAGAACAATGGAAGGAGAAATCCGTTTTCGTTTCTGTGGCCATTCCGTTCTTCTTCGTCTAGTTCAAACAATAATCAATATTCTGAGGGAAGCACTTCTTCAACCAATGCTGACCAGAATAACGGAACTAGTTACCATGGATGGCACGATAACTAAAAAAGAACTTCTGCATTTCTTCTGGTGGATTAAGTATATCACTGCATGTTGAATAATGCTTGAAATTTGTAATGAGATGGTAAGAGTATATTAATATTGTAATTCAACTTTTTCTCATGTAAGGGAAATGGGAGATAGAAGTATGTTCAAAATCTTTGTACATGTTATTATTTAGTAAGTTGAATCTCTTTGAAGCTTTCACATGTCATTATRYAATTTGTTTGTTTCCTTTCC
TARGET=761,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04532 Ps_contig_mira-and-tgicl-ass_franssen_33570
SEQUENCE=AGTGATAAATCCNCTCCACCATATTCATACAGGAGAGTTATCATATCACATTCACCGCTCTTGCTTACACACTTCCTTTGACTGGAACCTTGAGAAGTGACTATGGCGGTTCAAGATGATCCCATGGTGGTTCCCAATCTCTCTCCGCCCAATACCTACGAAGCTGTTGTCAATGGTGGCACCTTTGACCGATTGCATGACGGCCATCGCCTCTTTCTCACCGCTTCTGCTCAGTTGGCGAAGAACCGTGTTGTCATTGGAGTTTGCGATGGTCCTATGCTTGTAAAGAAACAGTTTGCTGAACTCATACAACCAATTGAGACGAGGATACAAAATGTCAAAACTTTCATAAAGTCTATTAAGCCAGAGTTGGAAGTGCAAGCTATACCAATTACAGATCCATATGGCCCTTCCATTGTTGATGAAAAATTGGAAGCTGTGATTGTGAGCAAAGAGACTTTACCTGGTGGGTTGGCTGTGAACAGAAAAAGAGCTGAGAGAGGCCTTTCACAGTTGAAGATTGAAGTTGTAGATTTGGTCTCCGGAGATTCAGGTGAGACTAAGCTGAGTTCATCAATGTTGCGAAAGCTTGAGGTTGAAAATGCCCAAAAACAAAGTTCAATACCAAAGACAGAGTAGTAACAAAGATTTGTTGACATGTATAAAAACAAAGACCGAGTCTAAGATGTCCATTAAAGCAACTAATAAGCAGGTGCATCAGAGTCGAAGATGTACATTAATGCAACTAATAAGCAGGTGTAGTTCAATGATGTATTTATTTATTTTTTTAAGTTAAATAATCACGTGTAACAAAAAGATTATGGTTTTGTTAGGACACATATTTTCTTCCTCTTTGAAATACCTTTGATTAATAAGTAAATATTAATGTTGCCTTGCAACAAATTAARAAATATGTTTGAAACCC
TARGET=773,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04533 Ps_contig_mira-and-tgicl-ass_franssen_33582
SEQUENCE=CGTTCATGGAGTTCGTACAGTCCAACGAAGACCGNGGTTTTGGRTATCAGTGGTGAGGACAAGGCCAGAATCACCTTGAACACACATGGGTCTATCAATAGAATTCATCATAGTCGGGGGATTCTTTGATTGGAACATTCTTTCCATTTCTCATTATATTTTTCTCTCTCTCTTGTTTATGAGAAATTTAGCTGAAAATAGATACAGATTCAAGAACAAATATGTGATCCCG
TARGET=163,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04534 Ps_contig_mira-and-tgicl-ass_franssen_33613
SEQUENCE=GGAACACCATAGAAAANNAAAAGCAAAGATTATGAAGGTAGATGGAGAAACCTTCAAAGTAAAACTCCAACCACATCAANCGACGTCGTCAAAGACTATCCCAACCACGTTCTCCTCGACTCTCAAGCCGTAAAACACTTCGGGCTTCGGGCCAAACCTATTTTAGAGCCCAACCAAGAAATTAAAGCCCAAGAAAATCTACTTCCTCGTAGATTTACCCAAAATTAAGCCCGAAGAAGATAAACCGTCACTACCGAGAAGAGCGCAATCTAGTGGAATAAGGAACATGAATGCTAAGGATAGACTCGAGTTGTTAATGCTATCAAAACGCTCCGTTTCGGATATTTCGTCGGTGAAACCGTTACCGGGTTTGGGCTTAGATGGGCCCATGAGGGTCAAGATGAGGCTTCCTAAAGCCCAGTTGGAGAAGTTGATGGAAGAGAGTAATGATAAGGCTGAGGTGGCGGAGAAGATTATGAGTTTGTATATGGAAACGAGTGGTGGTGGTGGTGATGATGCGGTGGAGCATGACGGTGTCGTTTTGAGTCAAAATCGTAAGCCACGTGGGAAACGAGTRAGTTTTAGCCCARTGGAAATTGAAGAAAGTCACGTAGCAGCAGCTTCTCAATAGCAAGTATTTTTTTAATTTAGTAGTACTTCATGATACTTAGATATGTACATTTGTACCGGAATACATAAATACATATATGTATATATAAAATGCAGGAATGATTTAATAAATACGAAGACCTAAGACGAATTTTAAAAGT
TARGET=498,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04535 Ps_contig_mira-and-tgicl-ass_franssen_33614
SEQUENCE=CTTTTTTTTTTYAATCACCAAATTTAAATATTAGAGTTTTTTTGAATGTTTTAGTTGCCACTTTATACAAACATTTTACATAAAGTAGAGTCCATAAATGCATATCATGTCCGGACTGCACATGTAAATAACGGTACACAACAAGTTACATATAGCGGGTTTTAAGCAGGAAAAGAAAACAAAGATATCCAAAGACTCAGTCATGCATCTTCAAAGGCTTCTATAAGGCACCATGTTCCATGCTATTATGAGCTTGCTAAGCCTGCAAATTACCATGAGAGCAACATCCTGTGACCTACAAAATGAAAAGCCATGCATCACATAACTCAAATTTGGCAAAGATGGAAAAAAACAACAAAGCCAATAGCATGCCACACAGTTCGTTTCTAAAGAAATGCCCAATCCTAAATGACCTTCATATGTTGCTTACTACCCTTCACTGAAATTATTATTACAGGCAAAGCATATTGGGTTATCATGGTAAGAGGGAAGTAATCAATATGTCATATAAAAAAATATAATAATTGCGGTAAAATAGAGGTTGCACCGAGTATATTCTAATCGCGTGGGACCACAAGAAACGCCAAAGGATGTACTGGATACATACAACACCGATTTCCCTCATAACAATTTCACAAATCTGGATCCCCACGAAATCTGGGATGCACAAAGGATTTCATAACAGATTTATCCTAAGATTACCAGCTCATCATTAAGGGAATATCCTTCACGATCTCCCCATAAATACCAAACCATGACGCCAAACAGAGGATACGGTTATTCTGCCAAAAAGTCCCAAGAACCTTTTCATAAAAGCTCACGAGTTTTCAAGTCTAAATTAGAGTTTCACACTGAAACTCTAGCATAGACGAGCTCAGACTAGCACTTTAATTAATAGCATTCAAAAGAAAATAGAGAAGAACAGAGAAGAAGAAGACGGAGAGAAATCAAAATTAGAAAGAGACTTATCTCAAACATAATGGAGAAGGCGTCGCCGGCGACTTGACTCCGATCAAGTATACCCTTTTATTCCTTTCTTTGCTTAAACTCTTTGCATCATCTTGCTCGCCTTGATGAACTGATTGAAACCCCCTTTGTAATTATTTAAAATCTCCGTTATTGTTGATTCAATTCCGAAATTAGGGTTTTCACTTTTGGTTTGTGTTTTTGTTGATTTCGGCTCCGGTTTCGTGTTTAGGAGAAGATTGAGCCTAGGTTTTGTACTGTGTGATTTAAGTTGTGGAATTTGACTTTTTTTTTGGATGGATGTCGATCTAGTCAGAGGAAGTTTGTTTTGTTAGATTTAAGACCGGATTCGTGTTCCTCGACCAAAAATAGGATGGGTTAGTGTTTAGGTTTTGTTGGTTTGGGTGTGGGTTCGCCGGAGAAGAAGGTGGCGTCGCCGTCGTTGGCCGACAACCACCGTCTTCTCCGGTAGGTTGCGGCCGCAGAATTATTTAGGGTTGTAGAGCTTATGGAATGTTGGGGATTGAAGAGAGAGAAGGGATTCATCAAA
TARGET=926,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04536 Ps_contig_mira-and-tgicl-ass_franssen_33643
SEQUENCE=CCTAGCTGCTTCCTAACCCCCAACTACCTGGTTTAGGTACAATTTGGTAGCGAGTGAAAAGGGAACAGGATCACGACTGAAAGTTAGGGTGAGGAATTGCAGGCAAGTTACATCCAATGCTTATATAAACAGGCTTGCTCTCTTTCAGTGCAGTTGAGATTGCTGTATCAATCAACTCATGAGCATCTTCCAAATTATTCACCACAGCCTGGAAGCAAGTAACCGTTTGAAAACACCTCAACTCTTGGCTAAAATCAGGTAACCCAATGGTATGATGAAGAATCCTGTTACTTCCATAATCATTGGAGTTTGGTCCACCAGCTATACAAATAAGCGGCAAATTCTCACTATAAGCTCCAGCTATAGCATTAAGAACACTAAGTCCACCAACAGTGAACGTAACCACACAAGCACCGACACCGCGTGATCGAGCGTAACCGTCGGCGGCATAGCCCGCATTGAGCTCGTTACAGCAACCGATTAAGTTAAGCCCCGGTTCATCGATGAGGTGATCGAGAAGCGTGAGGTTGAAGTCACCGGGAACGGAGAAAACGTCGGTGACGCCGACTTGGACTAGCCTACGTGCTAGGTGGCGGCCTAGCGTAGCGTCGGTGGAGGAAAACGGTGTTGATGGAATCGACGGCTGGATGCAGGATGTACTTGTTTGCTTTAGCGCATGAAACGACGTCGTTGCTGGTTGGTTTGCTTAAATCTAAAGAGCCTAACCTTGTGTCCATTTTGTGAAAGGTTTGGTTTGGTTTGGTTTAAAAGT
TARGET=747,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04537 Ps_contig_mira-and-tgicl-ass_franssen_33649
SEQUENCE=CCACCACCATCATAACCACCGCCAAACGCCAAAAACACTACCATCACCACCACCACCACCACCACAAAGATCATCATCATCTTCTCATACCTGGTCTCCCTGATCACATAGCTCAACTATGTCTCTCTTCAATCAACCCTTCTCTTCTCTTCAAAGTATGTCATTCATGGCGTAGACTTATCTATTCCCCTTCTTTTCCACCTTTCTTCTCTCTCTACGCCATTCTCTCTCCACCCAAATCACACCATTCCCATTCAATTCAATTCCACAATTTTGATCCCATATCAAACACCTGGCAGATCCTCCCTCCACCGCCACAAAACACATCACTCCAAAACATCCTCCTCCACCACCTTCCTTCCTCTCTCGAAATCTCTCCGTTCAATCAATCTCCGTCTCCGACAATCTCATCCTCCTCGCCGCCACAACGCACAACCTCTCCCCAGCACTCTCCCATCCTTTGATCTTCAATCCATACAAGGGTTGGTCATCCGGACCAGCCCTCGCTAATCCGCGCCGATGGTGTGCATTAGGCACATCCCATGACGTGGTCTATGTAGCAAGTGGCATCGGCTCTCATTTCTCCATCGACGTAGCCAAATCGATGGAGACCTGGAACCCGATAAACAATAGTAATTGGGAGAAGAAAACCGAGATGAAAGACGGAAGGTTTAGTAGAGAAGCTATTGATGCTGTTGGAG
TARGET=45,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4202
PRIMER_LEFT_PENALTY=0.276218
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=AAACGCCAAAAACACTACCATC
PRIMER_RIGHT_SEQUENCE=TGGCGTAGAGAGAGAAGAAAGG
PRIMER_LEFT=23,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.4353
PRIMER_LEFT_1_PENALTY=0.276218
PRIMER_RIGHT_1_PENALTY=0.159038
PRIMER_LEFT_1_SEQUENCE=AAACGCCAAAAACACTACCATC
PRIMER_RIGHT_1_SEQUENCE=AGGTGGAAAAGAAGGGGAATAG
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=202,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=59.841
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.4492
PRIMER_LEFT_2_PENALTY=0.276218
PRIMER_RIGHT_2_PENALTY=0.173020
PRIMER_LEFT_2_SEQUENCE=AAACGCCAAAAACACTACCATC
PRIMER_RIGHT_2_SEQUENCE=AAGGTGGAAAAGAAGGGGAATA
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=203,22
PRIMER_LEFT_2_TM=60.276
PRIMER_RIGHT_2_TM=60.173
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=181
PRIMER_PAIR_PENALTY_3=0.6551
PRIMER_LEFT_3_PENALTY=0.276218
PRIMER_RIGHT_3_PENALTY=0.378922
PRIMER_LEFT_3_SEQUENCE=AAACGCCAAAAACACTACCATC
PRIMER_RIGHT_3_SEQUENCE=GGAGAGAGAATGGCGTAGAGAG
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=231,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.621
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.6792
PRIMER_LEFT_4_PENALTY=0.276218
PRIMER_RIGHT_4_PENALTY=0.402961
PRIMER_LEFT_4_SEQUENCE=AAACGCCAAAAACACTACCATC
PRIMER_RIGHT_4_SEQUENCE=GTGGAGAGAGAATGGCGTAGAG
PRIMER_LEFT_4=23,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=60.403
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_04538 Ps_contig_mira-and-tgicl-ass_franssen_33649
SEQUENCE=CCACCACCATCATAACCACCGCCAAACGCCAAAAACACTACCATCACCACCACCACCACCACCACAAAGATCATCATCATCTTCTCATACCTGGTCTCCCTGATCACATAGCTCAACTATGTCTCTCTTCAATCAACCCTTCTCTTCTCTTCAAAGTATGTCATTCATGGCGTAGACTTATCTATTCCCCTTCTTTTCCACCTTTCTTCTCTCTCTACGCCATTCTCTCTCCACCCAAATCACACCATTCCCATTCAATTCAATTCCACAATTTTGATCCCATATCAAACACCTGGCAGATCCTCCCTCCACCGCCACAAAACACATCACTCCAAAACATCCTCCTCCACCACCTTCCTTCCTCTCTCGAAATCTCTCCGTTCAATCAATCTCCGTCTCCGACAATCTCATCCTCCTCGCCGCCACAACGCACAACCTCTCCCCAGCACTCTCCCATCCTTTGATCTTCAATCCATACAAGGGTTGGTCATCCGGACCAGCCCTCGCTAATCCGCGCCGATGGTGTGCATTAGGCACATCCCATGACGTGGTCTATGTAGCAAGTGGCATCGGCTCTCATTTCTCCATCGACGTAGCCAAATCGATGGAGACCTGGAACCCGATAAACAATAGTAATTGGGAGAAGAAAACCGAGATGAAAGACGGAAGGTTTAGTAGAGAAGCTATTGATGCTGTTGGAG
TARGET=70,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4202
PRIMER_LEFT_PENALTY=0.276218
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=AAACGCCAAAAACACTACCATC
PRIMER_RIGHT_SEQUENCE=TGGCGTAGAGAGAGAAGAAAGG
PRIMER_LEFT=23,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.4353
PRIMER_LEFT_1_PENALTY=0.276218
PRIMER_RIGHT_1_PENALTY=0.159038
PRIMER_LEFT_1_SEQUENCE=AAACGCCAAAAACACTACCATC
PRIMER_RIGHT_1_SEQUENCE=AGGTGGAAAAGAAGGGGAATAG
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=202,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=59.841
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.4492
PRIMER_LEFT_2_PENALTY=0.276218
PRIMER_RIGHT_2_PENALTY=0.173020
PRIMER_LEFT_2_SEQUENCE=AAACGCCAAAAACACTACCATC
PRIMER_RIGHT_2_SEQUENCE=AAGGTGGAAAAGAAGGGGAATA
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=203,22
PRIMER_LEFT_2_TM=60.276
PRIMER_RIGHT_2_TM=60.173
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=181
PRIMER_PAIR_PENALTY_3=0.6551
PRIMER_LEFT_3_PENALTY=0.276218
PRIMER_RIGHT_3_PENALTY=0.378922
PRIMER_LEFT_3_SEQUENCE=AAACGCCAAAAACACTACCATC
PRIMER_RIGHT_3_SEQUENCE=GGAGAGAGAATGGCGTAGAGAG
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=231,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.621
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.6792
PRIMER_LEFT_4_PENALTY=0.276218
PRIMER_RIGHT_4_PENALTY=0.402961
PRIMER_LEFT_4_SEQUENCE=AAACGCCAAAAACACTACCATC
PRIMER_RIGHT_4_SEQUENCE=GTGGAGAGAGAATGGCGTAGAG
PRIMER_LEFT_4=23,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=60.403
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_04539 Ps_contig_mira-and-tgicl-ass_franssen_33650
SEQUENCE=ACAAAAAACAATAAAAACAAATTGAAAACCGAACCGAAACCCAGTAGGAACACAATCTCAAGCTGATTCATAAATAAAGAATGAACTTAGGTTTTAATTTTGGTCCGAATACATTTTCATTGTCAACTACTACTACTACTAAATTTTCACCTTCCTTCATTTTCACCACTCCAATCTCTCACCCCCAAACCAATTCTTCAGGTACAAAAACTTTACTTCGTGTGCAAAACACTGATAATCATATTCACTCAACACCTACCACTACTCCTTTCATGGCTTCACAACAGAGAATTCCACAACTTAACCCTAACTTTTATCCAGATATTCAACCCTATACTACTGGTTTTTTGAAGGTTTCAGATCTTCATTCAATCTACTGGGAGCAATCAGGCAATCCAACTGGCCATCCAGTTGTTTTCCTTCATGGAGGCCCTGGAGGGGGAACTTCCCCGAGTAATAGGAGATTTTTCGATCCTGAGTTTTACCGAATCATACTATTTGATCAGCGAGGTGCAGGGAAAAGCACACCCCATGCTTGCTTAGAGCATAACACCACATGGGACCTAATCGACGACATTGAAAAATTAAGAGAACATTTGGAGATTCCCGAATGGCAGGTGTTCGGTGGATCATGGGGAAGCACACTTGCCCTTGCTTACAGCCAATCTCACCCCGACAAGGTTACTGGCATGGTCCTCAGAGGAATCTTTCTTTTAAGGAAGAAAGAGATCGATTGGTTTTATGAGGGTGGTGCTGCTGCAATATTCCCCGATGCTTGGGAGCCATTCAGAGATCTTATTCCAGAAAATGAGAGAGGATGCTTCATTGATGCTTATAAGAAGAGATTAAACTCTGATGATATCGAAACTCAGTATGCCGCGGCTAGAGCATGGACCAAATGGGAAATGATGACTGCTCATCTTTTTCCAAATGAAGAGAATGTCAAAAGAGGAGACGATGACTACTTTTCACTGGCATTTGCAAGGATCGAAAACCACTATTTCGTCAACAAGGGATTCTTTCCCTCCGATTCATTCTTGTTAGACGGAGTTGATAAAATAAGGCATATCAACACCACAATCGTGCAGGGAAGATACGATATTTGCTGCCCTATTATGTCAGCCTGGGATCTTCATAAAGCTTGGCCAGAGGCAGATTTTCGGGTGGTAGCCGATGCAGGACATTCAGCCAATGAACCGGGCATAGCTGCTGAACTTATCGCGGCAAATGAGAAATTGAAAAACATACTCAAGAATAAAGGGGACTAAAGTGAGTGAACAACCAAATACACACAAAAGGCACAGTTGGTGATTAATCAGAAACATCCTTAGCGAGCTTTTTTGTCTGCAAAGAAATAAATTATAAATGTGTATTGCAATTTTGTAAAATTTGCCTTCTTTCTCGTGCATGCACGAAAATTTTAATGCCAGGTGGTTTGGATCGTTCATAACTTGTTGAGTTTTATCATCATATCGAAATCAACTCAGACTAAAAATGTTCTTTGATCGGATTGTTCAGTTGAGTTTTAGTCATTGTATTGAATCAACTCAGCTAAAAATGTGCCCTTGATTGGATTAGTTTATAATTTGTTGTTTTAATCAGTGTATCGAAAT
TARGET=126,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2983
PRIMER_LEFT_PENALTY=0.274768
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=CGAAACCCAGTAGGAACACAAT
PRIMER_RIGHT_SEQUENCE=GAGTGGTGAAAATGAAGGAAGG
PRIMER_LEFT=34,22
PRIMER_RIGHT=170,22
PRIMER_LEFT_TM=60.275
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=0.2983
PRIMER_LEFT_1_PENALTY=0.274768
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=CGAAACCCAGTAGGAACACAAT
PRIMER_RIGHT_1_SEQUENCE=GGAGTGGTGAAAATGAAGGAAG
PRIMER_LEFT_1=34,22
PRIMER_RIGHT_1=171,22
PRIMER_LEFT_1_TM=60.275
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=0.3611
PRIMER_LEFT_2_PENALTY=0.274768
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=CGAAACCCAGTAGGAACACAAT
PRIMER_RIGHT_2_SEQUENCE=CTCTGTTGTGAAGCCATGAAAG
PRIMER_LEFT_2=34,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=60.275
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=0.4051
PRIMER_LEFT_3_PENALTY=0.274768
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=CGAAACCCAGTAGGAACACAAT
PRIMER_RIGHT_3_SEQUENCE=GAAGCCATGAAAGGAGTAGTGG
PRIMER_LEFT_3=34,22
PRIMER_RIGHT_3=279,22
PRIMER_LEFT_3_TM=60.275
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=0.4958
PRIMER_LEFT_4_PENALTY=0.274768
PRIMER_RIGHT_4_PENALTY=0.220992
PRIMER_LEFT_4_SEQUENCE=CGAAACCCAGTAGGAACACAAT
PRIMER_RIGHT_4_SEQUENCE=ATTGGAGTGGTGAAAATGAAGG
PRIMER_LEFT_4=34,22
PRIMER_RIGHT_4=174,22
PRIMER_LEFT_4_TM=60.275
PRIMER_RIGHT_4_TM=60.221
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_04540 Ps_contig_mira-and-tgicl-ass_franssen_33655
SEQUENCE=ATGGTTTTATATATTTCACCCGGTTGGACGAAATTAAGGAGTGAGCGAAGATTCTAGAAGACGCGTTACAGTAGTAGAATAAGGGGAAGTTTGTTAGGTAGAGAGAGAGACATTGAAGGCGCAGACATAGTTAGAGAGAGAGAGAAAGGTGAGTAAGGGTTTATCGATTCCAAAAATAAGCGACGGAAACAATCTAGCGACGGAAACAATCTAGCGACGAAATATGTCTGGTATTGCTCGTGGACGCCTTGCCGAGGAACGAAAGTCATGGCGAAAGAATCATCCTCATGGTTTCGTTGCCAAGCCTGAAACGCTCCCTGATGGAACTATCAATTTGATGGTCTGGCACTGCACTATTCCTGGCAAGATCGGGACTGATTGGGAAGGTGGCTATTATCCATTGACAATGATCTTCAGTGAAGACTACCCTAGCAAGCCACCTAAGTGTAAATTCCCTCAAAGTTTCTTCCACCCTAATGTGTATCCGTCTGGAACTGTTTGCTTGTCTATACTTAATGAGGATAGTGGGTGGAGACCAGCTATAACAGTGAAGCAAATTCTTGTGGGTATTCAAGACCTGCTTGATCAGCCAAATCCTGCTGACCCTGCCCAAACAGAAGGCTATCATCTATTCATCCAGGATGCAGTGGAGTATAAAAGAAGGGTTAAGCAGCAATCAAAGCAATATCCACCTCTTGTCTAGTACTATATATGCTCACTTTAGGAACTGTTCTAGTATGGTAGATGTTGTTTTCTATTTCTTGCCCGGTGCCAAAATTGTGTGATTGTTGGCTGCCATAACATGCAGACTCCTTGGTTTTAACTATTTTTATTTACTCAGTTCTGGATAACATGTTAATACGTGACAATGTGAAAGTCAACCAATAATACTTGTTGGCAATACTTGCTGGTTCATTATAAAAATGGATAATTTACTTCTATAAGA
TARGET=100,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1563
PRIMER_LEFT_PENALTY=0.128591
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=GGTTGGACGAAATTAAGGAGTG
PRIMER_RIGHT_SEQUENCE=ATTGTTTCCGTCGCTAGATTGT
PRIMER_LEFT=21,22
PRIMER_RIGHT=209,22
PRIMER_LEFT_TM=59.871
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.1678
PRIMER_LEFT_1_PENALTY=0.128591
PRIMER_RIGHT_1_PENALTY=0.039178
PRIMER_LEFT_1_SEQUENCE=GGTTGGACGAAATTAAGGAGTG
PRIMER_RIGHT_1_SEQUENCE=TCTAACTATGTCTGCGCCTTCA
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=134,22
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=60.039
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=0.2646
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=0.135999
PRIMER_LEFT_2_SEQUENCE=GGTTGGACGAAATTAAGGAGTG
PRIMER_RIGHT_2_SEQUENCE=GTTTCCGTCGCTAGATTGTTTC
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=206,22
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=60.136
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=0.2842
PRIMER_LEFT_3_PENALTY=0.256503
PRIMER_RIGHT_3_PENALTY=0.027715
PRIMER_LEFT_3_SEQUENCE=ACGAAATTAAGGAGTGAGCGAA
PRIMER_RIGHT_3_SEQUENCE=ATTGTTTCCGTCGCTAGATTGT
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=209,22
PRIMER_LEFT_3_TM=60.257
PRIMER_RIGHT_3_TM=60.028
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.2957
PRIMER_LEFT_4_PENALTY=0.256503
PRIMER_RIGHT_4_PENALTY=0.039178
PRIMER_LEFT_4_SEQUENCE=ACGAAATTAAGGAGTGAGCGAA
PRIMER_RIGHT_4_SEQUENCE=TCTAACTATGTCTGCGCCTTCA
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=134,22
PRIMER_LEFT_4_TM=60.257
PRIMER_RIGHT_4_TM=60.039
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_04541 Ps_contig_mira-and-tgicl-ass_franssen_33655
SEQUENCE=ATGGTTTTATATATTTCACCCGGTTGGACGAAATTAAGGAGTGAGCGAAGATTCTAGAAGACGCGTTACAGTAGTAGAATAAGGGGAAGTTTGTTAGGTAGAGAGAGAGACATTGAAGGCGCAGACATAGTTAGAGAGAGAGAGAAAGGTGAGTAAGGGTTTATCGATTCCAAAAATAAGCGACGGAAACAATCTAGCGACGGAAACAATCTAGCGACGAAATATGTCTGGTATTGCTCGTGGACGCCTTGCCGAGGAACGAAAGTCATGGCGAAAGAATCATCCTCATGGTTTCGTTGCCAAGCCTGAAACGCTCCCTGATGGAACTATCAATTTGATGGTCTGGCACTGCACTATTCCTGGCAAGATCGGGACTGATTGGGAAGGTGGCTATTATCCATTGACAATGATCTTCAGTGAAGACTACCCTAGCAAGCCACCTAAGTGTAAATTCCCTCAAAGTTTCTTCCACCCTAATGTGTATCCGTCTGGAACTGTTTGCTTGTCTATACTTAATGAGGATAGTGGGTGGAGACCAGCTATAACAGTGAAGCAAATTCTTGTGGGTATTCAAGACCTGCTTGATCAGCCAAATCCTGCTGACCCTGCCCAAACAGAAGGCTATCATCTATTCATCCAGGATGCAGTGGAGTATAAAAGAAGGGTTAAGCAGCAATCAAAGCAATATCCACCTCTTGTCTAGTACTATATATGCTCACTTTAGGAACTGTTCTAGTATGGTAGATGTTGTTTTCTATTTCTTGCCCGGTGCCAAAATTGTGTGATTGTTGGCTGCCATAACATGCAGACTCCTTGGTTTTAACTATTTTTATTTACTCAGTTCTGGATAACATGTTAATACGTGACAATGTGAAAGTCAACCAATAATACTTGTTGGCAATACTTGCTGGTTCATTATAAAAATGGATAATTTACTTCTATAAGA
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1563
PRIMER_LEFT_PENALTY=0.128591
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=GGTTGGACGAAATTAAGGAGTG
PRIMER_RIGHT_SEQUENCE=ATTGTTTCCGTCGCTAGATTGT
PRIMER_LEFT=21,22
PRIMER_RIGHT=209,22
PRIMER_LEFT_TM=59.871
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.1809
PRIMER_LEFT_1_PENALTY=0.153161
PRIMER_RIGHT_1_PENALTY=0.027715
PRIMER_LEFT_1_SEQUENCE=AGAGAGAGAGACATTGAAGGCG
PRIMER_RIGHT_1_SEQUENCE=ATTGTTTCCGTCGCTAGATTGT
PRIMER_LEFT_1=99,22
PRIMER_RIGHT_1=209,22
PRIMER_LEFT_1_TM=60.153
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=0.2646
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=0.135999
PRIMER_LEFT_2_SEQUENCE=GGTTGGACGAAATTAAGGAGTG
PRIMER_RIGHT_2_SEQUENCE=GTTTCCGTCGCTAGATTGTTTC
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=206,22
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=60.136
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=0.2842
PRIMER_LEFT_3_PENALTY=0.256503
PRIMER_RIGHT_3_PENALTY=0.027715
PRIMER_LEFT_3_SEQUENCE=ACGAAATTAAGGAGTGAGCGAA
PRIMER_RIGHT_3_SEQUENCE=ATTGTTTCCGTCGCTAGATTGT
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=209,22
PRIMER_LEFT_3_TM=60.257
PRIMER_RIGHT_3_TM=60.028
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.3229
PRIMER_LEFT_4_PENALTY=0.128591
PRIMER_RIGHT_4_PENALTY=0.194358
PRIMER_LEFT_4_SEQUENCE=GGTTGGACGAAATTAAGGAGTG
PRIMER_RIGHT_4_SEQUENCE=AACGAAACCATGAGGATGATTC
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=59.871
PRIMER_RIGHT_4_TM=60.194
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=277
=
PRIMER_SEQUENCE_ID=SSR_04542 Ps_contig_mira-and-tgicl-ass_franssen_33657
SEQUENCE=TTTAATTAAAATACGTGTTTATTATCAAATAAAAATCAATATACAACAAAGAACCAACCAATGAAAGGGATGCATTATTGTGTATATATATATTCCTCCTCTACATTGATACATCAAGAGAGAACCACCGAGCATTGAATCTTACAACTCAAAAAACAATTGGAAATTCCCTACATCCCTCCCACTCTACCCACATATGAAAAAAGGGACAACAAAAACATAAACCTAAGTTTTTAGTACCTTCCCTTTTAAGATGCCCTGGGATCAGTTCCATGCTGCCAAACATAAGCAAGTTCATCCCAACCAGTACTAGCAATAAGCCCTTCAACAAGAACACTCATATCAACCCCAACCGCAAACTCAGTATGATGATCATATCTACTCACCAAAGCATCCTCCACCATGAAATCCCAAACACAAACAGACATATCATAAGAACACGACACCATCAAATTCCGGACGTGCGGAGAAAATTTCACCTTTCTAACAGCATAACTATGCCCATTAAGCACCGAAATCGGAACCCTAAAACTCCTAACATCCCAAACCTTAATCGATTTATCAACAGATGCAGTAGCAATAATACATTCATCATACTTATTCCAATCACATGAAAGAATCTCGTGATCGTGTGCGGGGAGGATCATAGTGGAACCTGGCTCACGAACGTCCCAGATTCGGAGAGTACAATCACCGGAAGCGGAAGCAAAAACATCAGCGTGACGAGGGTTCCAAACGGCGGAGTAAACACAGTAAGCGTGCTCTTTGAAGGTACGAACGGAGGTAGGACGATCGACAGTCCATAGCTTGACAGTATCATCCCATGAGGCGGAGAGGAAGGAGTCACGGCGGACGGGGTTGTAGTCCGCAGAGTGAACTTCGCGGGTATGTTCGTGGAAGGACCGGATAGGGTTTGAAGTTGGGGGAAGAGCGAGGTCATAGAGTTTGACGGAGCCATCAGCTACGGCGGCGACTACGACGGAATCGTGAGATTCAGACCAGGTGACGTCGTAGACTCCATCGGCGGTGTCGTAGGCGATGAGTTCGGTGATTGGGAGAGAGGGATTTGAGGAGAGTTCGAGGACGTGGAGACGGCCGTTGCCGAGGATGCCGAAGTTTTGGGCGGTGGCGACGGCGATGCGATTTTCGTAGAATGGACTGAACTTGACGGAGTAACCGTTGAATGGGGTTTTGAAAACCGGCATGGTTTGATGAGAGTTCTGAGGATTTGGTTTGGGTTAAGTGAAAAGAGTTGTCAGCTAGTAATTGTGTGCATAGCAAGATTGAACATTTTTAGATTCCCCAGATTCCGTAGAAGAAAATGATTGGGCTAATTTCATCCTTTCCTCCCCCGNGTA
TARGET=83,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8532
PRIMER_LEFT_PENALTY=0.722278
PRIMER_RIGHT_PENALTY=0.130883
PRIMER_LEFT_SEQUENCE=CAAAGAACCAACCAATGAAAGG
PRIMER_RIGHT_SEQUENCE=TTGTAAGATTCAATGCTCGGTG
PRIMER_LEFT=46,22
PRIMER_RIGHT=146,22
PRIMER_LEFT_TM=60.722
PRIMER_RIGHT_TM=60.131
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=0.9788
PRIMER_LEFT_1_PENALTY=0.722278
PRIMER_RIGHT_1_PENALTY=0.256503
PRIMER_LEFT_1_SEQUENCE=CAAAGAACCAACCAATGAAAGG
PRIMER_RIGHT_1_SEQUENCE=TCTTGTTGAAGGGCTTATTGCT
PRIMER_LEFT_1=46,22
PRIMER_RIGHT_1=332,22
PRIMER_LEFT_1_TM=60.722
PRIMER_RIGHT_1_TM=60.257
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=1.0200
PRIMER_LEFT_2_PENALTY=0.722278
PRIMER_RIGHT_2_PENALTY=0.297699
PRIMER_LEFT_2_SEQUENCE=CAAAGAACCAACCAATGAAAGG
PRIMER_RIGHT_2_SEQUENCE=TGAACTTGCTTATGTTTGGCAG
PRIMER_LEFT_2=46,22
PRIMER_RIGHT_2=296,22
PRIMER_LEFT_2_TM=60.722
PRIMER_RIGHT_2_TM=60.298
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=1.0905
PRIMER_LEFT_3_PENALTY=0.722278
PRIMER_RIGHT_3_PENALTY=0.368190
PRIMER_LEFT_3_SEQUENCE=CAAAGAACCAACCAATGAAAGG
PRIMER_RIGHT_3_SEQUENCE=TGCTAGTACTGGTTGGGATGAA
PRIMER_LEFT_3=46,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=60.722
PRIMER_RIGHT_3_TM=59.632
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=1.0905
PRIMER_LEFT_4_PENALTY=0.722278
PRIMER_RIGHT_4_PENALTY=0.368190
PRIMER_LEFT_4_SEQUENCE=CAAAGAACCAACCAATGAAAGG
PRIMER_RIGHT_4_SEQUENCE=TTGCTAGTACTGGTTGGGATGA
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=315,22
PRIMER_LEFT_4_TM=60.722
PRIMER_RIGHT_4_TM=59.632
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_04543 Ps_contig_mira-and-tgicl-ass_franssen_33666
SEQUENCE=TTTTNNNGTGGAACTAGTTTTTTCTATTACAGTCATTGTCATCAATACAAATTGTCACGACAATCATACACACTTAGCACTAATATCTTCATCACACTACTTTATTTAACTAAATAACTTAATCATTCTTGAATCTCAGTGTTTCAATTTTCTAGACAGAATCTAAAACATGTTTCATATTCTCACATAAATAATTGACATTATTCAGACAATAATGAGCAACCAAAAAAAATTATAACCAGACAGTACTACCGAAAAATGAAAAAAGAAAAATGGCATATTTGACTCAATCTCATTTAAGAAACTAGAACTCAACTTTCCTATTCAAAACTTGGCTCCTGCTCTTTGCACAATCCTGAGTGGATAGAGTAATTTGGCTTCTCAGTTTGTGCTTCTCAACGATTAAAAGTACGTAATCCACGACGAGAGGAATCATAAGTTCTTTTGCTCTTGCGTATGCAGTATTAGAATACAGAAAAATGAGACTAGAAAGTAAGATCTTTAGGGTCTTCAATGATCTGTGACAGGGTTTGCAAGAACAGAGCTAGATCGGAACCGTAGATTACTCGATGATCTGCCGTAACATTGACCTGCATTTGGTTCTTCAATCCAATGCGACCATCCTTGGTAGCCACAACAGTTGGCTGTGATGATCCAACTGCCATAATTGCTCCAGTTCCAGGAGGCAGAATAGCATCGAACCGATCAACGCCAAACATTCCCAGGTTGGAAAGGGTGAAAGTACCTGTGGTGTATTCATGAGGCTGCAACTGCTTAGCTCGGGCCTTATCAACCAACTCCTTCCACTTTCTTGACAAAGAATAGACATCAACCTTATCAGCATCCTGAAGCACTGGAGTAATAAGTCCGCCATCTATAGCCACAGCAACTGCAATGTTGATGCTGCTATTGTAAGTAAAGCTATTACCATCCCTACAACTAGAGTTTATAACAGGGTGTTTAGCCAGCGCGAGTGCTGTAGCTTTTGCAAGCAATGCGGTCATAGTAACTCCCTTTGATTTAATCTTCTTGTAGAGAGCATCAAGTGCATCAGTGGTGAAAGTGTAACCAACTCTGAAAGTGGGAACAACCAAACTCTCCGCCATGTTCCTACTCACAGCATTCTGCATTGTCGTAAACGGCACCACAGTTCCCAATTCAACACCTGAAGCTGTAGTCTTCGCCGGCTCTGTCGCCGCCGCAGCAACACTAGCAGAAGAACCTTCTGAAGCTGCAAATGCCTCAACATCTTTAGCCACAATCCTCCCTGAAGGCCCAGTCCCAACAATTAGCCCTAACTCCACCTTCAATTCCTTAGCCAATTTCTTTGCAAAAGGAGATGCTACTATTCTCTTCCCTCCCTCTGACGCCGGATGTTTAGACACAACCGCCGCTGCAACAGCTTTCTTCGGTTGAGATTCTACAGGTGCGGGTGCAGGTGCGGGTGCAGGCGCCGGAGTAGAAGAAGATGGTGAAGAGGATGAAGATGAAGAAGAAGAAGAGAGGGCTTTAGACCTGGCTTGTTCGATTTCTTCTTCAGTCTCAGCGAGAAACGCGATGGGAGATCCAACGGCAGCGACGCCACCTTCTTCAACAACAATAGCAGCGAGGATACCATCATAGAAAGTCTCGACATCCATGTCAGCCTTGTCGGATTCAACGACGACAACGCTATCGCCTTTGGAGAGTTTGTCGCCTTCGGATTTGATCCACGAGACGATTTTGCCCTCGGTCATAGTGGAGCTAAGTGCAGGCATGAAAATCTCTCGGATCTTGGCACGGACGACGAAGTTGTTGGTGGAGGTTGAAGTGCGGTGGAGTGAAGTGGGAGTGCGGCGGAGGGCGGTGGAGGAATGGAGGAACGGAGTTCGAAGAAGGTGAGACATTGTTGAAGGTGAAAATGGGGGATTGGATTGAAATGAGGAATGTGAATTTGTGTTGTTTTTGTTTAAGAGAAATAGAAAGGATGTTTCCTCGAGTTCATCGAAAGATGGCGGAGGTTTCAACTACTTCTCTCACTGAATCTNACGTCACTTACCTACAACTGT
TARGET=1489,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2300
PRIMER_LEFT_PENALTY=0.122257
PRIMER_RIGHT_PENALTY=0.107763
PRIMER_LEFT_SEQUENCE=TTAGCCCTAACTCCACCTTCAA
PRIMER_RIGHT_SEQUENCE=AAGAAGAAATCGAACAAGCCAG
PRIMER_LEFT=1288,22
PRIMER_RIGHT=1536,22
PRIMER_LEFT_TM=60.122
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.2514
PRIMER_LEFT_1_PENALTY=0.122257
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=TTAGCCCTAACTCCACCTTCAA
PRIMER_RIGHT_1_SEQUENCE=AAATCGAACAAGCCAGGTCTAA
PRIMER_LEFT_1=1288,22
PRIMER_RIGHT_1=1530,22
PRIMER_LEFT_1_TM=60.122
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.2514
PRIMER_LEFT_2_PENALTY=0.122257
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=TTAGCCCTAACTCCACCTTCAA
PRIMER_RIGHT_2_SEQUENCE=AATCGAACAAGCCAGGTCTAAA
PRIMER_LEFT_2=1288,22
PRIMER_RIGHT_2=1529,22
PRIMER_LEFT_2_TM=60.122
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.3388
PRIMER_LEFT_3_PENALTY=0.122257
PRIMER_RIGHT_3_PENALTY=0.216588
PRIMER_LEFT_3_SEQUENCE=TTAGCCCTAACTCCACCTTCAA
PRIMER_RIGHT_3_SEQUENCE=ATCGAACAAGCCAGGTCTAAAG
PRIMER_LEFT_3=1288,22
PRIMER_RIGHT_3=1528,22
PRIMER_LEFT_3_TM=60.122
PRIMER_RIGHT_3_TM=59.783
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=0.3863
PRIMER_LEFT_4_PENALTY=0.372557
PRIMER_RIGHT_4_PENALTY=0.013714
PRIMER_LEFT_4_SEQUENCE=CAGCTTTCTTCGGTTGAGATTC
PRIMER_RIGHT_4_SEQUENCE=ATGGTATCCTCGCTGCTATTGT
PRIMER_LEFT_4=1399,22
PRIMER_RIGHT_4=1617,22
PRIMER_LEFT_4_TM=60.373
PRIMER_RIGHT_4_TM=60.014
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_04544 Ps_contig_mira-and-tgicl-ass_franssen_33671
SEQUENCE=ACAGTGGTAGTCAACGCAGAGNATGCGGGTAAAGCCTTACGTCTTTGTTCAAACGACGACAGAAGAGGGAAGAATCAATCGAGGGTTTCATTTCTCCTCCTCCTCCTCCTCACAATTACCCTATGAATCAATCATCCACACTCTGAAAATCCCAAATTTCTCACTCGCTTTGTAGGATGGATTCAAACTCACAGTCTCCGCCGTGTGGCGATTCTGACACCAAAAATGTATTTCGCAAGCCTTCTGGGGAGGCAGCTAATAGGAATTATCGGCGCCGATCACCTATTGATGGGTCATCATCACCTGATGGTAGTCCCAGGAGAGAACACAGCTCAAGTCCATATCCATCAAGGGAAGATTCTATGAGAGTCTCTCACCATCATTCAAGGAAGCTTGACGGAAGGGAACAAGACCGAAAATACGGCAGAAATCAGTATGGAAGAAGCAGTGATTCTCTTAGACATTCTGACAGACAGTCATTCCGGAGCTCCTATGGTAACTCAAGACATGACAAGTATGCAGACGAGGATAGAAAACATGAGAGGTTATCATCTCGTTATGCACGTGAGTCAAGGGGAGATCATATCAGAGAAGAGAGTGATAGCAGATCAAAAGACTATGGGCGTAGTATGGATAAGTATTCACGTGAAAAATATGAAAGGTCTGGATCTGGCAAGAGGTGTGCGGAATATGATGAGGTGGAGAAGGAGAGGCGTACGAGGGACGGGGATGGTCGAGATGAAAGAAAGGATTCTCAAAGAAGTTCCGGAGAACACAAAAGTGACCGTGCTGTTTCCTATTCAGAATCTAGGAGCCAAAATGATGACATTGAGATTAGTAAGAACAAGAACCAAAACTCTAGAAAGGTAGGTGGGGTGTTTGGTATTGAAGATAAAGAATCTTTTGGAAAGAAACCGAAGTTGTTTGGTGCTGAGGATGACAATTCTGGAAAAGATGATGAAAGGAAGACTTCAAGGTCAAAGCTTTCTCATGAGAGCAAGCCATATGTAGGGGCTGCTAAGACCAGTGGTTTTGATAGTGGCAATGATCTAGATGCTGCAAAAGTGGCAGCCATGAGAGCTGCTGAATTAGTTAACAAGAACTTAGTAGGGCCTAATTGCTTGACTACGGACCAAAAGAAGAAACTGTTGTGGGGCAGCAAGAAGAGTACACCCACTGAAGAGTCTGGCCACCGCTGGGATATGGCAATGTTTGATCGCGAGCGACAGGAAAAGTTCAACAAACTCATGGTAAGTTTTGATTGAGTCGATTGCTATATTCTGACCCAACAATAACATGAGATTGTTTCGTTGTTCCTGAATGACTGACATAATCTGTTAGTGTTTCTTCACCTACTAACACATTTTGAGTCTGAGGTTGTACTCGTGCCTATGGCAAATTGTAGGGTGTGAAAGGAGAAGCCAAAGTGGAGCAGAGCTCCGACAATCAAAATGTCAACGACGAACTCCGTGCTGAGAAGCAGAAGGAACTCCAGCTGGATCTTGAGAAACAGTACACTGCGGGTCTCCGGCGAAGAGATGGCCGTACAGTTGGATTAGGTCTTTAAACGTCCACAAGCGGAATTTTGCATCTTTTGACCTTGGCAGTTATTTTGAGTATACCAAATATTCACTAGTGTTATTCACTGGATTAGGTCTTTAAACATTTAAACTTATCATGTGTTTGTTACATGTTTGGGAGTTTATCATGTGTATGATCTCATACTTTGAGGAGCTTATCACAGTCGTATAATTTGAATGTGACGGTTTGATGCTAGAATATATAATGGAGCTGGGAGAGGTGTGAGGAAACATAGACAGTGATAAGCTTTAAATTAGAAGTTTTGAAGTAATGTTATTTGATACTTGTTAAAGG
TARGET=94,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1863
PRIMER_LEFT_PENALTY=0.180765
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=GTCTTTGTTCAAACGACGACAG
PRIMER_RIGHT_SEQUENCE=TGTTCTCTCCTGGGACTACCAT
PRIMER_LEFT=40,22
PRIMER_RIGHT=327,22
PRIMER_LEFT_TM=59.819
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.2059
PRIMER_LEFT_1_PENALTY=0.200343
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=CAGAAGAGGGAAGAATCAATCG
PRIMER_RIGHT_1_SEQUENCE=TGTTCTCTCCTGGGACTACCAT
PRIMER_LEFT_1=59,22
PRIMER_RIGHT_1=327,22
PRIMER_LEFT_1_TM=60.200
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=0.2523
PRIMER_LEFT_2_PENALTY=0.246726
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=ACGACAGAAGAGGGAAGAATCA
PRIMER_RIGHT_2_SEQUENCE=TGTTCTCTCCTGGGACTACCAT
PRIMER_LEFT_2=55,22
PRIMER_RIGHT_2=327,22
PRIMER_LEFT_2_TM=60.247
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.2961
PRIMER_LEFT_3_PENALTY=0.200343
PRIMER_RIGHT_3_PENALTY=0.095771
PRIMER_LEFT_3_SEQUENCE=CAGAAGAGGGAAGAATCAATCG
PRIMER_RIGHT_3_SEQUENCE=TGATGGATATGGACTTGAGCTG
PRIMER_LEFT_3=59,22
PRIMER_RIGHT_3=349,22
PRIMER_LEFT_3_TM=60.200
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.3234
PRIMER_LEFT_4_PENALTY=0.317850
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=TTTGTTCAAACGACGACAGAAG
PRIMER_RIGHT_4_SEQUENCE=TGTTCTCTCCTGGGACTACCAT
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=327,22
PRIMER_LEFT_4_TM=60.318
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_04545 Ps_contig_mira-and-tgicl-ass_franssen_33673
SEQUENCE=AGTTCTGCTGAAAATAGCAGCCAATGGATCATCAGCACGTCCTCTCAAGCCAGCGTATGTTCCTCCACATCTTCGTAACCGGTCAATGGCACCGGCCGAGCCTCCTTCTATGGTGGCTAATGCTAATGACAGGGGGTTTGGGTAATTGGGGTAGTTCGTCTAACTTCAATAAGCCGGAGTTTGGTGCTGGTAGGCAAGGATATGGTTCGGGTGGTGGGTTTAACAATAGAGGAGGAGGAGGGGCAGTGAGGGAGCATGGTGGAAGGCGCGAGGTGAATCCTTTTGAGAATGATGTTAATGAGTCTTTTAGTGAGCAAGAGAACACGGGGATTAACTTTGATGCTTACGCTGATATCCCCGTTGAGACTAGTGGAGAGAATGTTCCTCCTCCTGTGAATACATTTGCAGAAATAGATTTAGGTGATGCGTTAAATCAGAACATACAGAGATGCAAGTATGTACGAAAACCCAACCCCAGTTCAAGGAGGTATG
TARGET=229,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1287
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=CCTCCTTCTATGGTGGCTAATG
PRIMER_RIGHT_SEQUENCE=CATCAAAGTTAATCCCCGTGTT
PRIMER_LEFT=100,22
PRIMER_RIGHT=341,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.1299
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.110131
PRIMER_LEFT_1_SEQUENCE=CCTCCTTCTATGGTGGCTAATG
PRIMER_RIGHT_1_SEQUENCE=TTCACAGGAGGAGGAACATTCT
PRIMER_LEFT_1=100,22
PRIMER_RIGHT_1=396,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.1345
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=0.108929
PRIMER_LEFT_2_SEQUENCE=AGCGTATGTTCCTCCACATCTT
PRIMER_RIGHT_2_SEQUENCE=CATCAAAGTTAATCCCCGTGTT
PRIMER_LEFT_2=51,22
PRIMER_RIGHT_2=341,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.1467
PRIMER_LEFT_3_PENALTY=0.143555
PRIMER_RIGHT_3_PENALTY=0.003166
PRIMER_LEFT_3_SEQUENCE=CTGGTAGGCAAGGATATGGTTC
PRIMER_RIGHT_3_SEQUENCE=TGTTCTGATTTAACGCATCACC
PRIMER_LEFT_3=186,22
PRIMER_RIGHT_3=440,22
PRIMER_LEFT_3_TM=59.856
PRIMER_RIGHT_3_TM=60.003
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.1484
PRIMER_LEFT_4_PENALTY=0.019805
PRIMER_RIGHT_4_PENALTY=0.128591
PRIMER_LEFT_4_SEQUENCE=CCTCCTTCTATGGTGGCTAATG
PRIMER_RIGHT_4_SEQUENCE=TCAAAGTTAATCCCCGTGTTCT
PRIMER_LEFT_4=100,22
PRIMER_RIGHT_4=339,22
PRIMER_LEFT_4_TM=59.980
PRIMER_RIGHT_4_TM=59.871
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_04546 Ps_contig_mira-and-tgicl-ass_franssen_33675
SEQUENCE=GGGGGAACCGTGAATATAATTATTGAAACCCTCAGGTTCCCAAAACCGCCCCTCCCTCTCTCTCCCTCTCCAAGCAGCTGAGGAGTTCGAGTGAGACTCGTAAGAAATCAGACATGTCTGGAAGGAAGGAAACTGTTCTGGACTTGGCGAAATTCGTTGATAAAGGAGTCCAAGTCAAGCTTACTGGTGGTAGACAAGTGACAGGTACTCTAAAAGGATACGACCAGTTACTTAACCTTGTCCTGGATGAAGCTGTTGAGTTTCTGAGAGATCCTGATGATCCTCTGAAAACTACAGATCAGACAAGAAGTCTTGGCTTAATTGTTTGCAGGGGAACTGCTGTGATGCTTGTGTCCCCAACTGATGGTACAGATGAGATTGCAAACCCCTTTATTGAGGCAGATGGGGCCTAGGTGTAAATACTAAAGTCTTTAAAATACAGAATCTGTATGTTTGC
TARGET=120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3169
PRIMER_LEFT_PENALTY=0.199477
PRIMER_RIGHT_PENALTY=0.117452
PRIMER_LEFT_SEQUENCE=AGCTGAGGAGTTCGAGTGAGAC
PRIMER_RIGHT_SEQUENCE=ATCTGTACCATCAGTTGGGGAC
PRIMER_LEFT=75,22
PRIMER_RIGHT=373,22
PRIMER_LEFT_TM=60.199
PRIMER_RIGHT_TM=60.117
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=0.3169
PRIMER_LEFT_1_PENALTY=0.199477
PRIMER_RIGHT_1_PENALTY=0.117452
PRIMER_LEFT_1_SEQUENCE=GCTGAGGAGTTCGAGTGAGACT
PRIMER_RIGHT_1_SEQUENCE=ATCTGTACCATCAGTTGGGGAC
PRIMER_LEFT_1=76,22
PRIMER_RIGHT_1=373,22
PRIMER_LEFT_1_TM=60.199
PRIMER_RIGHT_1_TM=60.117
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.3298
PRIMER_LEFT_2_PENALTY=0.199477
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=AGCTGAGGAGTTCGAGTGAGAC
PRIMER_RIGHT_2_SEQUENCE=AGCTTCATCCAGGACAAGGTTA
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=253,22
PRIMER_LEFT_2_TM=60.199
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.3298
PRIMER_LEFT_3_PENALTY=0.199477
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=GCTGAGGAGTTCGAGTGAGACT
PRIMER_RIGHT_3_SEQUENCE=AGCTTCATCCAGGACAAGGTTA
PRIMER_LEFT_3=76,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=60.199
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=178
PRIMER_PAIR_PENALTY_4=0.5173
PRIMER_LEFT_4_PENALTY=0.199477
PRIMER_RIGHT_4_PENALTY=0.317845
PRIMER_LEFT_4_SEQUENCE=AGCTGAGGAGTTCGAGTGAGAC
PRIMER_RIGHT_4_SEQUENCE=CCTGTCACTTGTCTACCACCAG
PRIMER_LEFT_4=75,22
PRIMER_RIGHT_4=204,22
PRIMER_LEFT_4_TM=60.199
PRIMER_RIGHT_4_TM=59.682
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_04547 Ps_contig_mira-and-tgicl-ass_franssen_33677
SEQUENCE=AAAGAGGTTTGGTAGCGTTAATGAAAGGATGTTATTTTGGTTGGTTGAGTGTTGATGATGTTCCTTCCTTGCTGCACTCATTCACTACTCTTCACTACTAAGCTCTCAATTCAACAACACTTTCGCTATTATCATCCATGAAGAACAACGATAACAACAACAACAACAGCAAAGACACTACCATAGGAATCCTCGATTCCCGCTTCAACCAAACCCTCAGAAATGTTCAAGGGTTACTCAAGGGTCGTAGCATTCCTGGGAAAGTATTGTTGAGCCCGAGGGATCCGATCGATAATTCGAGCTTATTCTCGCCGACTTACCAGAGGAGTTTGTCGTATAGTGATACCGGTACAAGTAATCACACAATTGAGACAGTAGAGGAGGAAGTTCAAAGTGGAGGCAAATCATTTGGTATTATGAGTGATAATAAATCGAAAACATCGACCTCGCATGTAGAGAATCCGTCTGAAGAAGTTCGGAAAACTTCCATGGGTGCTAGAGCGACTGATTCTGCAAGAGTTATGAAGTTCACAAAGGCGCTTTCGGGAACAATGGTTATATTAGACAAATTGCGTGAATTAGCTTGGAGCGGTGTTCCGGATTATATGCGTCCTACAGTGTGGAGAGTTCTCTTGGGATATGCACCAACTAATTCAGATAGAAGGGAGGGAGTTTTGAGAAGGAAGCGGCTCGAGTATCTTGATTGTGTTTCTCAGTATTATGATATTCCTGATACAGAACGCTCAGATGATGAGATCAGCATGCTTCGCCAGATTGGCGTTGACTGTCCAAGAACTGTACCAGATGTTTCATTCTTCCAACAACCACAAGTTCAGAAATCACTGGAGCGTATTCTTTACGCATGGGCCATACGGCATCCAGCAAGTGGATATGTTCAGGGGATAAACGACCTTGTCACGCCTATTTTTTGTTGTTTTCCTATCTGAATACTTAGAAGGAAGCATAGATAATTGGACAATGTCCGATTTATCTTCAGATAAAATCTCTAATGTAGAGGCTGACTGCTATTGGTGCTTGTCAAAATTGCTTGATGGTATGCAAGACCATTATACATTTGCTNCCGAAACCGGGTAATTACGAAAGGTCTTTGTTACG
TARGET=153,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1286
PRIMER_LEFT_PENALTY=0.029253
PRIMER_RIGHT_PENALTY=0.099308
PRIMER_LEFT_SEQUENCE=TGTTGATGATGTTCCTTCCTTG
PRIMER_RIGHT_SEQUENCE=ACCCTTGAGTAACCCTTGAACA
PRIMER_LEFT=49,22
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=59.901
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.1567
PRIMER_LEFT_1_PENALTY=0.057411
PRIMER_RIGHT_1_PENALTY=0.099308
PRIMER_LEFT_1_SEQUENCE=CCTTGCTGCACTCATTCACTAC
PRIMER_RIGHT_1_SEQUENCE=ACCCTTGAGTAACCCTTGAACA
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=244,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=179
PRIMER_PAIR_PENALTY_2=0.2837
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.254467
PRIMER_LEFT_2_SEQUENCE=TGTTGATGATGTTCCTTCCTTG
PRIMER_RIGHT_2_SEQUENCE=AACATTTCTGAGGGTTTGGTTG
PRIMER_LEFT_2=49,22
PRIMER_RIGHT_2=226,22
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=60.254
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=0.2981
PRIMER_LEFT_3_PENALTY=0.029253
PRIMER_RIGHT_3_PENALTY=0.268895
PRIMER_LEFT_3_SEQUENCE=TGTTGATGATGTTCCTTCCTTG
PRIMER_RIGHT_3_SEQUENCE=CTGGTAAGTCGGCGAGAATAAG
PRIMER_LEFT_3=49,22
PRIMER_RIGHT_3=322,22
PRIMER_LEFT_3_TM=59.971
PRIMER_RIGHT_3_TM=60.269
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.3086
PRIMER_LEFT_4_PENALTY=0.209271
PRIMER_RIGHT_4_PENALTY=0.099308
PRIMER_LEFT_4_SEQUENCE=CTCAATTCAACAACACTTTCGC
PRIMER_RIGHT_4_SEQUENCE=ACCCTTGAGTAACCCTTGAACA
PRIMER_LEFT_4=104,22
PRIMER_RIGHT_4=244,22
PRIMER_LEFT_4_TM=59.791
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_04548 Ps_contig_mira-and-tgicl-ass_franssen_33683
SEQUENCE=CTTGGGGCAAAACGCAGCAATAGCAAAGGCATATCGAGGGTTGAGTGGGTTAATCGATCAGAAAAATTTGCTGCTGCTAATTCCAATCGTGTTTCCGTCGTCATCGCCGCCATGGATTCTCTGCTAGCAACTTACGCCTCTTCAGACGAAGACGAAGACCACCAACAACCAATCCCGTCCAAAACAACTTCGTCTTCTTCCTCCCTCTTCCCCACTCTTCCTCCACCCAAATCATCTTCTTCCTCTTCTCTCTTCAACTTTCTTCCTCCTCCCAAACAACCCTCTTCCGACACACTTTCCGCCGCCCCCTCCAATTTGACCGGAATCTCTTCTCTCCCAAAACCTAAATCCCAAATCCATGAAAAACCCAAAAGAGTCGTCCAATTCAAACCCCCAATCATTCCTTTACCAAAACCCAATCTTGAAGAGGATGAAGATGATGACGAAGAAGAAGAACGAAACAGAAGAAGAAAGTTGGAGTCTTCCATTCAAGCTCCATCGGTGAAATCATTTTTGTCCAGCATTCCAGCCCCGCGAAACTCTTCCACTCTTGGTGTTCAATCAAGCTCAGGTTCCGGCCGGAGATCCATCCTCGAAACTAACACACCAGCACCGGAGCCAAGTTCCGGTGGTGTCTCTGTCTCAGTCGCCGCAGAGAGCAATGTTCCTGAAGATCAAAACCGAGGGGATTATGAAAATTATGAGAATTACCAATATGCCACTGACCAGTATGATAGTTATGGGAATTACCAATATCCAACTGACCAGTATGAGAGTTCTGGTGCTAGCACTGGCACTGCTTCCAATGGTGATGGGTATGCGAACTACGGTGCCTATGAGGATTATGGACAGTATGAAAATAAATGGGTTGATAGGTCGGTGCCAATGGCCCCGGAGGATTCTGCGATTAGTGAGAGTGTGTTGAAGTTTACTGGGAAGAGAGGTAGGAAGGAGGTTCCGGTGGAAGTGATTGAAGTGAAGCAAGATGAGTTGATGAAGAATAGACCAAGAGAGGATAAAGCCAAGCTAACTGGGTTAGCTTTTGGACCTTCTTACCAGCCTGTTTCAGCAAAGGGAAAGCCTTCAAAGCTAATGAAGAGGAAGCATCAAATTGGTTCATTGTACTTTGATATGAAACAAAATGAAATGAAACTCGCCGAGCGCCGTTCCAAGGGCATGCTTACCAAAGCTGAAACACAAGCGAAATATGGATGGTGATTTGGGGATGATGCTGTAATTTTAAGCACTCCATTTGTAAAATGATTTACAAAGCTAGCTTTCAAATGTAGCTGCAGCACTTGTGCTATACTGTTCTGTATGGATACATTTACAGATTTACTCCAATTTTCAGTGTAAAGAATAAATTTATTATCATATATTCTTATTGTGGAATTTTAGTAAAAAGG
TARGET=445,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0902
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=ACCCAATCTTGAAGAGGATGAA
PRIMER_RIGHT_SEQUENCE=TGGTGTGTTAGTTTCGAGGATG
PRIMER_LEFT=413,22
PRIMER_RIGHT=608,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.0902
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=AACCCAATCTTGAAGAGGATGA
PRIMER_RIGHT_1_SEQUENCE=TGGTGTGTTAGTTTCGAGGATG
PRIMER_LEFT_1=412,22
PRIMER_RIGHT_1=608,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=0.0978
PRIMER_LEFT_2_PENALTY=0.067516
PRIMER_RIGHT_2_PENALTY=0.030276
PRIMER_LEFT_2_SEQUENCE=CCCAATCTTGAAGAGGATGAAG
PRIMER_RIGHT_2_SEQUENCE=TGGTGTGTTAGTTTCGAGGATG
PRIMER_LEFT_2=414,22
PRIMER_RIGHT_2=608,22
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=60.030
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.1027
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.042816
PRIMER_LEFT_3_SEQUENCE=ACCCAATCTTGAAGAGGATGAA
PRIMER_RIGHT_3_SEQUENCE=ATAATCCCCTCGGTTTTGATCT
PRIMER_LEFT_3=413,22
PRIMER_RIGHT_3=692,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=60.043
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.1027
PRIMER_LEFT_4_PENALTY=0.059875
PRIMER_RIGHT_4_PENALTY=0.042816
PRIMER_LEFT_4_SEQUENCE=AACCCAATCTTGAAGAGGATGA
PRIMER_RIGHT_4_SEQUENCE=ATAATCCCCTCGGTTTTGATCT
PRIMER_LEFT_4=412,22
PRIMER_RIGHT_4=692,22
PRIMER_LEFT_4_TM=59.940
PRIMER_RIGHT_4_TM=60.043
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_04549 Ps_contig_mira-and-tgicl-ass_franssen_33693
SEQUENCE=ACACCACGTGTGCCACTTTCCGTCTATCGTTTGCTTCATAGCCCCTTCTTTTGTTGGACAGAGACAGCAACGTATTGGATCGGTGCTTGTAAAACGACACCGTTCGCAGAACCAATCACCCTCTGGGATTTGTTTTGCTAGTGGGTTTCCATAGCAGGAAGCGTGGACCATTAGGTTACAACCGTCGCAAAACACTATTGGATCTTCGGAGTTAGCGTCGGTGCTCTGACACACACAGCATAAAATCCCATCCTCTTCAGCGAGAACTTCTTTGCTGTTGTTGTTGTTAGTGTCTTGTTG
TARGET=276,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04550 Ps_contig_mira-and-tgicl-ass_franssen_33694
SEQUENCE=AGTTAATGGGGACTAAGCTTTTGATTCAAGAGATGAAGGAGGAGAGAGGGAGAAGAGATGAAGCTGTTGAGAAATGGAAGCAACTTTATCTTGCAATTAAGAATGAGTTGGATGATCTCATTCAAAGAACTTATGATGGAGATGGTGTGTATTGGAAAACAGAAGAAAATGAGATTCAAATGGAGAATCTGAGGAATGAGTTGCAAGAGAAAGAAAGAAAGCATGAAAGCATTAAAAGACTAAAACTAGTTTCTGTGGAGAAAGAAAGGTGCAAACAAGAGAGAGAATTTGACTTGGTTTAAAGGACTAAAGTTTAGGAGGATC
TARGET=208,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3785
PRIMER_LEFT_PENALTY=0.227356
PRIMER_RIGHT_PENALTY=0.151106
PRIMER_LEFT_SEQUENCE=TGGAGATGGTGTGTATTGGAAA
PRIMER_RIGHT_SEQUENCE=GTTTGCACCTTTCTTTCTCCAC
PRIMER_LEFT=136,22
PRIMER_RIGHT=275,22
PRIMER_LEFT_TM=60.227
PRIMER_RIGHT_TM=60.151
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=140
PRIMER_PAIR_PENALTY_1=0.4748
PRIMER_LEFT_1_PENALTY=0.323721
PRIMER_RIGHT_1_PENALTY=0.151106
PRIMER_LEFT_1_SEQUENCE=GATGGAGATGGTGTGTATTGGA
PRIMER_RIGHT_1_SEQUENCE=GTTTGCACCTTTCTTTCTCCAC
PRIMER_LEFT_1=134,22
PRIMER_RIGHT_1=275,22
PRIMER_LEFT_1_TM=59.676
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=142
PRIMER_PAIR_PENALTY_2=0.5333
PRIMER_LEFT_2_PENALTY=0.382228
PRIMER_RIGHT_2_PENALTY=0.151106
PRIMER_LEFT_2_SEQUENCE=GGGAGAAGAGATGAAGCTGTTG
PRIMER_RIGHT_2_SEQUENCE=GTTTGCACCTTTCTTTCTCCAC
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=275,22
PRIMER_LEFT_2_TM=60.382
PRIMER_RIGHT_2_TM=60.151
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=0.7871
PRIMER_LEFT_3_PENALTY=0.636033
PRIMER_RIGHT_3_PENALTY=0.151106
PRIMER_LEFT_3_SEQUENCE=TGATGGAGATGGTGTGTATTGG
PRIMER_RIGHT_3_SEQUENCE=GTTTGCACCTTTCTTTCTCCAC
PRIMER_LEFT_3=133,22
PRIMER_RIGHT_3=275,22
PRIMER_LEFT_3_TM=60.636
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=0.8501
PRIMER_LEFT_4_PENALTY=0.227356
PRIMER_RIGHT_4_PENALTY=0.622772
PRIMER_LEFT_4_SEQUENCE=TGGAGATGGTGTGTATTGGAAA
PRIMER_RIGHT_4_SEQUENCE=TTGTTTGCACCTTTCTTTCTCC
PRIMER_LEFT_4=136,22
PRIMER_RIGHT_4=277,22
PRIMER_LEFT_4_TM=60.227
PRIMER_RIGHT_4_TM=60.623
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=142
=
PRIMER_SEQUENCE_ID=SSR_04551 Ps_contig_mira-and-tgicl-ass_franssen_33697
SEQUENCE=TCCAACTACTTCTACGTCTCACCGTCCACTGTACAATCGAATCAAATTTACGTCAGTAGAAAAAAAAGAAACAATAAGACATAAAAGAAAAAAGAACGAATTAATTAAGTCAAATGGCTTATCAYCATGACCACCTATCACATGAAATTAACTTTCAACCATTCACAGAAGAGCAACAGTTAAATCAAAGCAGAGACATGCAAAGGCTACTACCGTTCACCGGCGCCGGAGCTGGATCAGCGCCGAACTGGCTCAACAACGCCGTAAACCTACGGCAACAGAATTTCCTCCACTTGCAACCAGACACATCGCAAAACGACGACGGAAGAGGAATTCACATGATGGAAAAAAGCCGAGATCGAAACCGGACGGAAAGCAGCAACATAGAATCAGAAGAGTTATCACAATACAAAGCAGATATTCTTGGACATCCTCTCTACGATCAGCTTCTATCTGCTCACGTGTCGTGTCTGCGAATCGCTACGCCGGTCGATCAGCTTCCTCGGATCGACGCGCAGCTTCAGCAGACGCAGCGTGTGGTGGATAAGTACTCCTCACTTGCTAACGGTGTTGTAGATGAGAAGGAGCTTGATCAATTCATGACGCATTATGTTCTCCTGACTTTAGTGCTTTTAAAGAACAATTGCAACAGCATGTCCGTGTCCATGCCATGGAAGCTGTTATGGCTTGTTGGGAATTGGAGCAGTCTCTACAAAGCTTGACTGGTGTATCTCCGGGTGAAGGAACTGGTGCAACAATGTCAGACGATGAAGACGATCAAGCAGAGAGCAATGCAAACTTATACGAAGGAAGCCTTGATGGCGGTGATAGTCTCAGCTTTGGTCCACTTGTTCCCACTGAAAGTGAAAGATCTTTAATGGAACGTGTGAGGCAGGAGTTAAAGCATGAGCTAAAACAGGGTTACAAGAGTAAGATTGTGGACATAAGAGAAGAAATTCTACGCAAGAGAAGAGCGGGAAAGCTTCCTGGTGATACCACCTCCCTTTTGAAAGCTTGGTGGCAGTCCCACTCAAAATGGCCTTATCCTACTGAAGAAGACAAAGCAAGGTTGGTCCAGGAAACAGGACTTCAATTAAAGCAAATAAACAACTGGTTCATTAATCAAAGAAAAAGGAATTGGCACACTAATCCCTCATCTTCCACTGGCTCCAAAACAAAGCGCAAGAGTGGCGCAGGTGAAACAAGTAACCAGAGCTTCATGTGAGTCGATATCATGTTTTGATGAAAGAAAGAATTATTAATATTCTTCATTTTATGCAACTATATATGTTGGGACGCTTCATGTATAGGACAAGAGCTTATAGTGAAAAATGATGTGATGTGATGGAATATGAATGTCAAATCCAAATTTGTTTATAACATTGCCAAGAAATGTTACTACATTACATTACTTGGTAATTATGTTTTTAAAAGTTATTTTAGAAATAAATAATAGTTTCACTTTGTTAAAAAATACATTGCAAAATAAAATAAAATTACTTGTATGTANTTTTTTTTAC
TARGET=1333,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04552 Ps_contig_mira-and-tgicl-ass_franssen_33697
SEQUENCE=TCCAACTACTTCTACGTCTCACCGTCCACTGTACAATCGAATCAAATTTACGTCAGTAGAAAAAAAAGAAACAATAAGACATAAAAGAAAAAAGAACGAATTAATTAAGTCAAATGGCTTATCAYCATGACCACCTATCACATGAAATTAACTTTCAACCATTCACAGAAGAGCAACAGTTAAATCAAAGCAGAGACATGCAAAGGCTACTACCGTTCACCGGCGCCGGAGCTGGATCAGCGCCGAACTGGCTCAACAACGCCGTAAACCTACGGCAACAGAATTTCCTCCACTTGCAACCAGACACATCGCAAAACGACGACGGAAGAGGAATTCACATGATGGAAAAAAGCCGAGATCGAAACCGGACGGAAAGCAGCAACATAGAATCAGAAGAGTTATCACAATACAAAGCAGATATTCTTGGACATCCTCTCTACGATCAGCTTCTATCTGCTCACGTGTCGTGTCTGCGAATCGCTACGCCGGTCGATCAGCTTCCTCGGATCGACGCGCAGCTTCAGCAGACGCAGCGTGTGGTGGATAAGTACTCCTCACTTGCTAACGGTGTTGTAGATGAGAAGGAGCTTGATCAATTCATGACGCATTATGTTCTCCTGACTTTAGTGCTTTTAAAGAACAATTGCAACAGCATGTCCGTGTCCATGCCATGGAAGCTGTTATGGCTTGTTGGGAATTGGAGCAGTCTCTACAAAGCTTGACTGGTGTATCTCCGGGTGAAGGAACTGGTGCAACAATGTCAGACGATGAAGACGATCAAGCAGAGAGCAATGCAAACTTATACGAAGGAAGCCTTGATGGCGGTGATAGTCTCAGCTTTGGTCCACTTGTTCCCACTGAAAGTGAAAGATCTTTAATGGAACGTGTGAGGCAGGAGTTAAAGCATGAGCTAAAACAGGGTTACAAGAGTAAGATTGTGGACATAAGAGAAGAAATTCTACGCAAGAGAAGAGCGGGAAAGCTTCCTGGTGATACCACCTCCCTTTTGAAAGCTTGGTGGCAGTCCCACTCAAAATGGCCTTATCCTACTGAAGAAGACAAAGCAAGGTTGGTCCAGGAAACAGGACTTCAATTAAAGCAAATAAACAACTGGTTCATTAATCAAAGAAAAAGGAATTGGCACACTAATCCCTCATCTTCCACTGGCTCCAAAACAAAGCGCAAGAGTGGCGCAGGTGAAACAAGTAACCAGAGCTTCATGTGAGTCGATATCATGTTTTGATGAAAGAAAGAATTATTAATATTCTTCATTTTATGCAACTATATATGTTGGGACGCTTCATGTATAGGACAAGAGCTTATAGTGAAAAATGATGTGATGTGATGGAATATGAATGTCAAATCCAAATTTGTTTATAACATTGCCAAGAAATGTTACTACATTACATTACTTGGTAATTATGTTTTTAAAAGTTATTTTAGAAATAAATAATAGTTTCACTTTGTTAAAAAATACATTGCAAAATAAAATAAAATTACTTGTATGTANTTTTTTTTAC
TARGET=1483,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04553 Ps_contig_mira-and-tgicl-ass_franssen_33700
SEQUENCE=ATATAAAATAAGAAAACCCACTTGTACCAATTTAATTTGAAAAATAAACAAGAAATGTTTGATACGCTTTCTCTACTTGGCCTATATTCCTTTTCCATTATCAACCTCACAACTCATCACAAACAGAATACACCTCAATTCTTCTCTCTCATAACAATCTCTCGTCTTCTCATAAATTAAAAACCCTTAATTATTATTTTTTGTTAGAAAAATACTTATTATTATTATTATCATCATGGCTCAAAGAACAGAAAATGAAGAAACTGAATTCAAAGTACCTGAAACGATATCGCTTTGCATCAACAACTGTGGCGTAATCGGTAATCCTTCAACGAACAATATGTGTCAAAATTGCTTCACCGCTTCAACCGTAACCACTTCTTCCACCGTCGGGATCGGAATAACCGGAACCGGAATCGCACCTCAATCTTCCAGATCTGTTCGATCTCCGAAGAGATCTCTACCGGAGGAATCGTCTTCGGTTGTTACAGATCGGACTTCGTCGGATCAACCGATGGTTTCCGAGGCCAAGCGTGTTGTTAGCCGATGCTCCGGTTGCCGGAGGAAGGTAGGGTTGACCGGATTCCGATGTAGATGCGGTGATCTTTTCTGCTCCGAACATCGTTACTCCGATCGTCATGATTGTAGCTATGATTATAAAGGTGCTGGAAGAGAAGCTATTGCGAGGGAGAATCCGGTTATCAGAGCAGCGAAAATCGTTAAGGTTTGAAAATCTGAAAATCTGAATCCGAAGAAGAAGATGAAGATGAAGAAATGATTCAAAACGTTAAATTCTGGAAATGAATCTATTTGACCCGGTTCTTATCGACCGGGTTCGGGTCACACGTGTTTATTTTATTTAAATTTATTTACTTTTCCAGATTTTATTTTTAAAAATAGGAAAAAAAATCGTTAACTTAAGATAGTTATTAAGAGAGGTTGTGTGTAACGTTACTAAAAAATGAAGAATCTGAATTTCTATGTGTTTGATNCTRNNNNRANCTTTTCATTTCTTTT
TARGET=216,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04554 Ps_contig_mira-and-tgicl-ass_franssen_33707
SEQUENCE=ATTTGTTTGTAAAGTTTGCATTCATGATCAAAGATTTCATTTAGGAGGAATAGCTATTTATCAAGAGATTCACGTTTATATCACCAGAGGCACAGAATATGCAAACCTCTTAATAAGAGTAATTCAATCTGTACAAAGATATACAAACCTGCTACATACAATTTACCATCTCAACTTCACTACCAATCCGTCGCAAACTCGAGGTTGGATACTCGTCGAAGACGTCGAAGAAAGAACTGCAACTCTATAAACTAATTTCACCAAATTGCTACATTCAAGGTTTATAAGACTGAAAACTCAAGGAACCCCTTTATCTTAGGTGAGAGTCATAAGGGTCGAAAAGGGAGTCGGGAATGATCAAAGGTAACTTATCTATGTACATGAAAAGTCTTCGGAGATTCTCCCTTGTCACTGTTTCCATGTCTACTACATCTCGACTATCGGTCAATACCCAGAGATCGCACGAGTCCACTCTTTTAAGACTGTCACGACAAAACGGGCAAGATTGTGATCTTGCTCTCCATTCATGGTAACATTTGAGGCACATGGCATGGTTGCAGTTGGGCAATACAATCTTACTATTCATCTCCATGCATATTCCACATTCTTCTTCTCTTTCAAGGTCTATGTCTGAGGAATGTTTGTGCCCCGCATCATCTCTTCTACGATACCTCTCCATGCATACAACCTTCTGTTTTTTATCTTCCGCATCAGTGACACCTTTTTCAAGTTGTATTAAAGACGGATATATGATCGCGTAGAATTCCCTAATACTTGCTTTTCTTTCATGTGTTGACATGGTAGTTGTTCCATCCACATACACCTTGTAAATTAGGATTCTTAATAGTCCAAGGGCTCCAGCAAGATTGCAATCTGTCCATTGTACCAGAAAAAGAAACAAGTGTGCTGCTGGACTGTATGACATTCTCATCTGAAGACACGCGCCATCATATTCCCTAGGAAAATCGGATGCTAAGGTATTGGCATGATGAATATCAGCTTCAAGGAGTTTAAGAGATTCCTTGAAAGACCTTCCCATGGAAGAAGAACCTCCACTTATGTACATATCTCCTCGTACTCCCTCCCAGATTATTGTCAAACCAAAATAGTAGTAATAGTTTGTTTTATCGATCAAAAAACAACAACAATTTTCATACTAGTTTTTTCTCTCTCTCTCTAGTGTAGAATCTAGAATGCGCCTTCYTC
TARGET=1166,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04555 Ps_contig_mira-and-tgicl-ass_franssen_33709
SEQUENCE=TCTCATCTTCCTCTTAATCAATACTAGAGAGAGAGAAAGACGAGAGAGNGTGATTTCGTAGCTTTCTACAATGGCAGCTTCTATGAAATGGTTTCTCAATTTGTTGTTACTTTTTTGTTGCTGCTCGTTGAAGAAGAAGCTATGGCTTCTACACAGATCTCTCAGTTCAGCAATGTGGATACTGAGACACACAGGTTAACAAGCTTTAACGATTCATCAATGGCGGCAAGGCAAAAAGAAGCTGAGAAATTGAATGAGCGTGCTGCCGTGGCTGATCCAAAGGAGGTCGTTTCTATGGTTGAGATGCACATTAAGAACAGTACTGAAAGAAGAAACCTTGGATATTTCTCGTGCGGAACGGGTAACCCGATTGACGATTGCTGGCGTTGCGACCCCAATTGGCAACAGAATAGGAAGCGTCTGGCAGATTGTGGTATTGGTTTTGGAAGAAATGCTATCGGTGGTCGCGATGGAAAGTTCTATGTTGTCACTGACCCGAGAGACGACGACCCTGTTAACCCAAGGCCGGGAACTTTGCGTCACGCTGTTATCCAAGATAGGCCACTTTGGATTGTGTTCAAGAGGGACATGGTTATTCAGTTGAAACAAGAGCTGATTATGAACAGTTTCAAGACTATTGATGGAAGAGGAGCAAACGTACATATTGCAAATGGAGCGTGCATTACGATTCAGTATGTTACTAATGTTATCATTCATGGTCTTCATATTCACGACTGTGTTCCTACTGGTAATGCTATGGTGAGAAGCTCGGAAACACATTTTGGTTGGAGGACTATGGCGGATGGTGACGCTGTTTCTATATTTGGTTCAAGTCATATTTGGGTTGATCATAATTCTCTGTCGCATTGCGCCGATGGTCTTGTTGATGCTGTCGTCGGTTCAACAGCTATCACAATTTCTAACAACCATTTCACCCACCACAATGAGGTGATTCTGTTGGGCCACAGTGACTCCTACACAAGGGACAAGCAAATGCAAGTGACCATAGCTTATAACCATTTCGGAGAGGGACTTATTCAGAGAATGCCTCGGTGTAGACATGGTTATTTCCATGTGGTGAACAATGATTATACTCACTGGGAGATGTATGCTATTGGTGGAAGTGCTGAGCCCACAATCAACAGCCAGGGTAACAGATACAATGCTCCTGTTAACCCTTTTGCCAAAGAGGTAACTAAGAGAGTGGAAACAGCTGAAAGCCAATGGAAAGGTTGGAATTGGAGATCAGAGGGAGATTTGTACCTAAATGGTGCATACTTCACAGCATCCGGTGCTGGAGCTTCAGCTAGCTATGCAAGAGCCTCAAGCTTAGGAGCAAAATCTTCAGCCATGGTTGGCACCATGACATCAAATGCTGGTGCACTAGGTTGCAAAAGAGGCCGTCAGTGCTAACAAAAACCATTTCATGGAATCATCAAAACAACCATTTTTCTGTGTCTGATTTTTCAGTTTTTTTTATAGTATGCTTTTTTCCTCTGCATCATCATGCATTCTTGACAAGTGTACATTAATTACACATTGTCATTATGTTATTATTGTGTGTCAAAACCAAAACCTAAACCTAAACCTAAACCAAAACCAAAACCAAAACATTATTAGCTGCTCAACTTGCTCCAGCTGAAAACAAACAGTGCAAAATTGTAGGAGCAGGTGCTGAAGCGCTTCTCTACTTTGGTCCTTCACCACCAACCTTACCCACCATCAATTCATATGCATATTTTTCCATATGTCAAATCATGTCTCCTTACACTGTTCAGTCCTTTTCAGTTTTATTTTATAGGATTTGAATCTCAAGAAGCCAAGATATATATAAATATTTTTACTTTTTTTTTTACTTTTGTTTTGTTTGTAGTTATTATACGAAGAAAAAAAGTAAAATGTNTTGTTCTAAAGTTAGTAGTAAGTAGGTAGGTAATAGGTAAACGAACCTTTACTTACTTATTA
TARGET=26,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04556 Ps_contig_mira-and-tgicl-ass_franssen_33710
SEQUENCE=AAGTTTTAGTTAAAAATAATATATATATACATTTCATGAGATCCAGAAATACAAAAGCATTCCACAAACTATGCAACAACATTGATGGCAAGAGGTTCCTAGACCGTACAAATACAGAACCCATCACCATGCCAAGAAATACAAGGGGTAGCATCCTCTGAATGTTAAAATGTGCCAACGCAAATGCTATTGAGCTAACTAATACTGCACTCCACACAGGCATGTATCTCGTTAAAGATGGGAGAAGGAAACCACGAAAGACAATCTCCTCCCATATTGGGGCACAAACTGAAACTACCACTGCATATAGGACCATTGCCACGGGGTCTCGTGCTACAATTGATTGCTCTACACTCGAGACTGTAACTGGAGCATATTGAAGAACGGGCAATAAATTTAGGTTCATTTGTGACAGATGATTGATGAGGGGGAACATGAGGCAGCCTAAACCGACATCAAACTGCCAGTTCCCTTTGAGTTCAAATCTGAACCAGTCAGCTGRAAGTGGTTTAAACTTTGCAAGACAGCGATGAAGTATAGCTATCCCAACCACGCCTTCGGCAACATCCGTCAAGAGACTGTACAATGCTTGTCCTCTGTATGAAAGAGTTTCTTTTCTAAAACCTGCTGTGTAAGCTAAAAACGGAACTATCCAAGAACCTACCAACCAGAATGAGGCAATCCAGAGGAGCATAACCTGAACAATGGTCTTTGCAGTACCAA
TARGET=19,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04557 Ps_contig_mira-and-tgicl-ass_franssen_33712
SEQUENCE=TACGTAATACACTTATAAGTGTCTCCTACATYATGATCAGATTAACTGCATATCAGATCAATTTTGTTTGTACCTTGAAAGCATTTATTTACAGCACAAGCCATTCCATTTTGAATCATATAACATACAACACACACTACTATTATGTTTGTAACTCAAAACATGTTTTAGGATCTCCAAATATTTCAGCTGAGAAAATTCATTACACTCAAAGATACAATTGAAGGATGTTAAAAGAATAAAAATTATTATTAATTAATTAATAATAATAATAATAATCACAATTAAGGTAACGCGAGAAATATCATTCGGGAGCCTCTGGTTTTTCCTTTATAAATGAGACAACACCACTCTGTGGTTCTAGCAAATGGTGATTCGAATCTTCCTCTTCACTATCATCTGACGCAGAATGCTCTTCAGATTCTGTTTCCTCTTCATCGGAATGTGCAAGAAGATCGGGTTGATGTCTGACAGCCTCGACCTGAAGACTTTCTGGCAGAAGACAATTGCAAAAAGAACCAACACGGGCCAACCGATTTACCCATGCAGGGATAGGCTTTCCGGTTAACTCTTGGCAAACTTCATCTGTAAAATGGTTGCAATTCTTAGCAATCAGATGGTATGTGTCTCCGTGATATTTAGTGGATAGGCGCTCGATGAAAGAACGGAATTGTGAATAGGACATATCAGTGCTGCCTAACAATACTGAACGTCGGAAGATAAAGCCAGGACAACTTCTAGGTTCCACCTCAAATACTCCACTCGTCGAGTACTCATGAGCTCCAAACCCATATTCCATGCCATGCACTTCTACACCAGAATGAAAGACCCCGACGCCAAACATGTAAAGATAATTATTTGCAGGCGTAAGATCATACACATTAAGGTACACCATAGCATTACTCTTTTTCTCGCTCTCTCGCACCCTTTCCAAGCTACCATTCCTTCGCAAAGACGGCATTGTGTAGGATTTCCTCCTTCTCTAATCCCCCAATTCTCTAATCCTCTAAATACATAACCCCATTCTTCTTCGCGTAGCGAAAGGAGAATGGGGAAGGGAAAGGGGTTGGAGAGTAATTTGGGGTGGAAGTTGAGGAAGAAACGTAAGGTGGCTCGAATGCGTTCCACAAAGCCTAAAATCAGACAAACTCTTCTTTCGCTATGCCTTTTGGGCGTTCCTCGCCACCCCCGCGTAA
TARGET=251,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04558 Ps_contig_mira-and-tgicl-ass_franssen_33712
SEQUENCE=TACGTAATACACTTATAAGTGTCTCCTACATYATGATCAGATTAACTGCATATCAGATCAATTTTGTTTGTACCTTGAAAGCATTTATTTACAGCACAAGCCATTCCATTTTGAATCATATAACATACAACACACACTACTATTATGTTTGTAACTCAAAACATGTTTTAGGATCTCCAAATATTTCAGCTGAGAAAATTCATTACACTCAAAGATACAATTGAAGGATGTTAAAAGAATAAAAATTATTATTAATTAATTAATAATAATAATAATAATCACAATTAAGGTAACGCGAGAAATATCATTCGGGAGCCTCTGGTTTTTCCTTTATAAATGAGACAACACCACTCTGTGGTTCTAGCAAATGGTGATTCGAATCTTCCTCTTCACTATCATCTGACGCAGAATGCTCTTCAGATTCTGTTTCCTCTTCATCGGAATGTGCAAGAAGATCGGGTTGATGTCTGACAGCCTCGACCTGAAGACTTTCTGGCAGAAGACAATTGCAAAAAGAACCAACACGGGCCAACCGATTTACCCATGCAGGGATAGGCTTTCCGGTTAACTCTTGGCAAACTTCATCTGTAAAATGGTTGCAATTCTTAGCAATCAGATGGTATGTGTCTCCGTGATATTTAGTGGATAGGCGCTCGATGAAAGAACGGAATTGTGAATAGGACATATCAGTGCTGCCTAACAATACTGAACGTCGGAAGATAAAGCCAGGACAACTTCTAGGTTCCACCTCAAATACTCCACTCGTCGAGTACTCATGAGCTCCAAACCCATATTCCATGCCATGCACTTCTACACCAGAATGAAAGACCCCGACGCCAAACATGTAAAGATAATTATTTGCAGGCGTAAGATCATACACATTAAGGTACACCATAGCATTACTCTTTTTCTCGCTCTCTCGCACCCTTTCCAAGCTACCATTCCTTCGCAAAGACGGCATTGTGTAGGATTTCCTCCTTCTCTAATCCCCCAATTCTCTAATCCTCTAAATACATAACCCCATTCTTCTTCGCGTAGCGAAAGGAGAATGGGGAAGGGAAAGGGGTTGGAGAGTAATTTGGGGTGGAAGTTGAGGAAGAAACGTAAGGTGGCTCGAATGCGTTCCACAAAGCCTAAAATCAGACAAACTCTTCTTTCGCTATGCCTTTTGGGCGTTCCTCGCCACCCCCGCGTAA
TARGET=261,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04559 Ps_contig_mira-and-tgicl-ass_franssen_33715
SEQUENCE=GGCGGGGGTGGAAAAATATATATTCCTTCGTATAAATAGAAACCAATGCTACAGACTTTTGAGGGTATAAACAGAATCTTCCTCAACTATAGCTTTGGCAATATCGACACATAGTATATATGGTTTAGCTTTGCAACTCAGGTGAATCAAATACAAATAGCAGAGGCAAAGCCAGTATCATGGTGATGGAAGAAGAAAGAGATTGTTGTTCAACAATAATAAGAAACAGAGGCAACAACAACAACGTCAACCCCAAAGAGAATCACAAACAAGAGAAACCATACAGAGGAATAAGGAAGAGAAAGTGGGGAAAGTGGGTAGCAGAAATTAGAGAACCAAACAAAAGATCAAGAATCTGGTTAGGTTCTTACACCACACCCGTAGCCGCCGCACGTGCTTACGACACCGCCGTTTTCTACCTAAGAGGTCCGACCGCTCGCCTTAATTTCCCCGAATTATTGTTCCAAGACGACGAAGACAACAAAAACGACAACAACGGTTCTGTTCCACAAGGTAACATGTCCGCTGATTCAATACGCAAGAAAGCCACCACGGTCGGTGCTAGAGTTGATGCTCTTCAAACCGCTCTTCAAGCTTCATCACGTTCTAGTTCTAATCAATTTAACTCTGATGTTAAACCTGATTTGAACGAGTTTCCAAAACCCGAAGATTATTAATATAATTGATACGGTTTATTTTCTCTATGTAATCTCTGTAATTTCCTCTACTCAGTTTCTCTTTCTGTTTCTTAGAATTGTTAACAGAGCATACATATGCAGAAATAGATTATAGAATATGGACTTTTGTCGTAAATTATTGTAACCTACTTTTATATTTAATTTCTGAAAATATTATNTATGAAATAANCATGTTTTGTTGAATTG
TARGET=233,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1103
PRIMER_LEFT_PENALTY=0.008262
PRIMER_RIGHT_PENALTY=0.102004
PRIMER_LEFT_SEQUENCE=GCTTTGGCAATATCGACACATA
PRIMER_RIGHT_SEQUENCE=CTTTCCCCACTTTCTCTTCCTT
PRIMER_LEFT=91,22
PRIMER_RIGHT=313,22
PRIMER_LEFT_TM=59.992
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=223
PRIMER_PAIR_PENALTY_1=0.2767
PRIMER_LEFT_1_PENALTY=0.174657
PRIMER_RIGHT_1_PENALTY=0.102004
PRIMER_LEFT_1_SEQUENCE=GGTTTAGCTTTGCAACTCAGGT
PRIMER_RIGHT_1_SEQUENCE=CTTTCCCCACTTTCTCTTCCTT
PRIMER_LEFT_1=121,22
PRIMER_RIGHT_1=313,22
PRIMER_LEFT_1_TM=59.825
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.3709
PRIMER_LEFT_2_PENALTY=0.008262
PRIMER_RIGHT_2_PENALTY=0.362666
PRIMER_LEFT_2_SEQUENCE=GCTTTGGCAATATCGACACATA
PRIMER_RIGHT_2_SEQUENCE=ACTTTCCCCACTTTCTCTTCCT
PRIMER_LEFT_2=91,22
PRIMER_RIGHT_2=314,22
PRIMER_LEFT_2_TM=59.992
PRIMER_RIGHT_2_TM=59.637
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.3857
PRIMER_LEFT_3_PENALTY=0.283676
PRIMER_RIGHT_3_PENALTY=0.102004
PRIMER_LEFT_3_SEQUENCE=CTTTGCAACTCAGGTGAATCAA
PRIMER_RIGHT_3_SEQUENCE=CTTTCCCCACTTTCTCTTCCTT
PRIMER_LEFT_3=128,22
PRIMER_RIGHT_3=313,22
PRIMER_LEFT_3_TM=60.284
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=0.5043
PRIMER_LEFT_4_PENALTY=0.402305
PRIMER_RIGHT_4_PENALTY=0.102004
PRIMER_LEFT_4_SEQUENCE=AATAGCAGAGGCAAAGCCAGTA
PRIMER_RIGHT_4_SEQUENCE=CTTTCCCCACTTTCTCTTCCTT
PRIMER_LEFT_4=155,22
PRIMER_RIGHT_4=313,22
PRIMER_LEFT_4_TM=60.402
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_04560 Ps_contig_mira-and-tgicl-ass_franssen_33718
SEQUENCE=AACACCCAACATGGGGTTTTGTAATTTATTTAGCATGTTGTTTTGTTTTGTTCCCTTTGTCAACATTCAAACTCTCTTATTTTTCATATCCAACGCTTTCGTTACAATAGTAAAATAAAATTAAACAAAAGATGCACAACTTAACATACAGTGTACATAAGTACAAGTAACAACTAACCAACATACAAAACGATGACAAAATTGTTTTGCTATTATTCTCTTCTGCAAATACAGAGAGCATTATTTGGAGATGCTATGAAATTAGTTATAACATCGATGACGAATACAACAATTACCATCGAACCATAATCATACATGTAAAGACATTTTTATTGCATCTTTTTTCACTTGCGCGCAAGTGGAAACAATATGCCTTAGCCATCCATTAATACTATATTAATACACACGAGCTTTAGGAGGGAATGACTCAACTTCTTCGTCCAATGTATTCAAATGCACCGGCCGGTAATCCAATCGGACCTTCTCATTCTCCCAGAATCCTAAAGTATGTTTCATCCACTTCTCATCGTCTCTTGTCGTGAAGTCCTCGCGGGCATGAGCTCCTCTACTCTCCTTCCTAGCTTCTGCAGAGTGCATTGTGATGCAGGCATTTATCAAAAGATTTTCCAATTCAATAGTCTCAATCAAGTCGGAGTTCCATATTAAACTCCGGTCCTCAACCTTGACATCATGGAAACTCTCCCACGTTTTATCAATTAATTGACAACCTTCTTCTAATGTTTCTTGTGTGCGGAATACAGCAGCATTACTTTGCATGACTCGTTGCATATTCAACCGAATTTTTGAAGTAGGTAAAGAACCATTTGAATTTCTCAATTTGTCCAACCATGCAATAGTTTTCTGGCCAGCATCCTTCTCTAAAGGCTTTTGTTTCTCTCCTGGCCTATTAATTTCAGCAACTCTATTTGCACAAGCTCTACCAAAAACAACAATATCCAAAAGTGAATTTGCACCAAGCCTGTTGGCTCCATGAACTGATGCACAGGCTGCTTCTCCAGCAGCCATCAATCCTGGAACTAATGCATCTGGATCGTCACCTTTAATGGTAACCACCTCTCCATGATGATTTGTGGGAATGCCACCCATGTTATAATGCACCGTTGGCAAAACAGGAATTGGTTCCTTTGTAACATCCACACCAGCAAAAATAGCAGCAGTTTCAGAGATACCAGGAAGTCTCTCCTTGAGCACATCTGGGGGTAGATGATTTAAGTGTAGATAGATGTGATCTTTCAGGGGTCCTACACCACGACCTTCCCGAATCTCCATGGTCATTGATCGTGAAACAACATCTCTGGAAGCAAGATCCTTTGCTGTGGGAGCATATCGTTCCATAAACCGCTCCCCCTCGCTATTCCTAAGAATTCCACCTTCTCCACGGGAGCCTTCTGTTATGAGACAACCTGCTCCATATATACCGGTTGGGTGAAATTGCACAAACTCTAAATCCTCAAGCCCGATCCCAGCACGTGCAGCCATAGCATTGCCATCTCCAGTGCATGTATGTGCTGAGGTTGCTGAGAAGTACGCCCTACCATAACCCCCTGTGGCCAAAATTGTTGAAGCAGCTTGGAAACGATGTAGTGTCCCATCCTCCATATTTAAGGCAATCACTCCTTGGCAAGTACCATCACTATTCATTAAAAGATCCAAAGCAAAATATTCCACAAAAAACTGCGTATTGTGTCTCATAGCCTGGCCATAGAGAGTGTGCAATAAAGCATGCCCAGTTCGATCAGCAGCACATGCACAACGGTAAGCTTGACCACCTTTTCCAAAGTTTAAGCTTTGGCCACCAAATGCACGCTGATATATTTTTCCATCCTCTGTTCGAGAAAAAGGCAATCCGTAATTCTCTAGTTCAATAACCGCTTTTGGCGCTTCCCTACACATATATTGGATGGC
TARGET=38,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4313
PRIMER_LEFT_PENALTY=1.411045
PRIMER_RIGHT_PENALTY=0.020269
PRIMER_LEFT_SEQUENCE=ACCCAACATGGGGTTTTGTAAT
PRIMER_RIGHT_SEQUENCE=TGTAACGAAAGCGTTGGATATG
PRIMER_LEFT=3,22
PRIMER_RIGHT=105,22
PRIMER_LEFT_TM=61.411
PRIMER_RIGHT_TM=60.020
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=103
PRIMER_PAIR_PENALTY_1=1.7083
PRIMER_LEFT_1_PENALTY=1.688076
PRIMER_RIGHT_1_PENALTY=0.020269
PRIMER_LEFT_1_SEQUENCE=CCCAACATGGGGTTTTGTAAT
PRIMER_RIGHT_1_SEQUENCE=TGTAACGAAAGCGTTGGATATG
PRIMER_LEFT_1=4,21
PRIMER_RIGHT_1=105,22
PRIMER_LEFT_1_TM=60.688
PRIMER_RIGHT_1_TM=60.020
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=5.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=2.0719
PRIMER_LEFT_2_PENALTY=2.051674
PRIMER_RIGHT_2_PENALTY=0.020269
PRIMER_LEFT_2_SEQUENCE=ACCCAACATGGGGTTTTGTA
PRIMER_RIGHT_2_SEQUENCE=TGTAACGAAAGCGTTGGATATG
PRIMER_LEFT_2=3,20
PRIMER_RIGHT_2=105,22
PRIMER_LEFT_2_TM=59.948
PRIMER_RIGHT_2_TM=60.020
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=2.0954
PRIMER_LEFT_3_PENALTY=1.411045
PRIMER_RIGHT_3_PENALTY=0.684395
PRIMER_LEFT_3_SEQUENCE=ACCCAACATGGGGTTTTGTAAT
PRIMER_RIGHT_3_SEQUENCE=CTATTGTAACGAAAGCGTTGGA
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=109,22
PRIMER_LEFT_3_TM=61.411
PRIMER_RIGHT_3_TM=59.316
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=2.2410
PRIMER_LEFT_4_PENALTY=2.220776
PRIMER_RIGHT_4_PENALTY=0.020269
PRIMER_LEFT_4_SEQUENCE=ACCCAACATGGGGTTTTGTAA
PRIMER_RIGHT_4_SEQUENCE=TGTAACGAAAGCGTTGGATATG
PRIMER_LEFT_4=3,21
PRIMER_RIGHT_4=105,22
PRIMER_LEFT_4_TM=61.221
PRIMER_RIGHT_4_TM=60.020
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=5.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_04561 Ps_contig_mira-and-tgicl-ass_franssen_33720
SEQUENCE=CTTTGTCTTGTTTGTTACTACCATTGTTGTTGTTGTTGATGTTGGAATTTTGTCAAGTACCTGCAAAGAACCTTACATTTAACAGCACTACCATAGACATAAAAACCAGGTGCAACCATTATCCTAACCATGTTTGGTACCAACCTTGAAGATTTGTCTCCACCCATATCTTCCATGTAAACAGAATCATAATTCACACCTTTATCAACTCTAAAAATTGTTAAACTTGGATGAACTGAATTCGCCAACAAATGAACCATCCAAACACTTTTTGAAGCAACAAAGAAAGCTTGCAATAGTGGTTCCGACCAAGCTCTATTCCAACCCAACATAGCAACAATCTCACTCATTTTTCTGTCGCAAAATCGACTAAAATCTTCGCTAAAATGTCTTGTTCCTTTGCTCAATACTTCGTCCCATGTTAAACCATGAATCATGTTGAACGAAGTGTAACTTGACTCACATCTTTCCATTGGGTTTAGGATTCTGTTACAACCGTTCTTTTGAAAAGCTATTGACTCAAAATCCTCGAAGAACGTCTTGTTCAAAAGTGCTTCTAGATAGAAAAGCAAACTTCTTGGATTCTTCGAAAACGAGATTTTTATATCATAGGGTTGTAAAAGTGATGAAACTTTCTCGTAAACTTTGTTTCCGGTGTGTCGAAGTTGCATTGTTAATGAACGACTTAGAAGCCTCACGGAAGATCTTGCTTCGGAAACCGACACCAAGAAATGTTGTACAACGTTGTTGTCGGTGTCTTGAAGAACATGATGGTGANTNAANATTTATTTTTTCGTAGGAAACGGAAAAACCCGGTATTAAGGTAAA
TARGET=24,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04562 Ps_contig_mira-and-tgicl-ass_franssen_33724
SEQUENCE=TATCCCCTCCGTTTCGTCGTGTCGTTCAGGTTTTAGTTAATTTAGTTGTTAGGTAAAAACGTAAAACTAAACTAGTAATTAGGTTATGTTTTTGTTTTATTTGTTGTTTTTTGTTCTATGTAGAGTATTATTATTAGCTACATANAGTGNTNTNATNNGCAGAGTTATGTGCAGTGCAGTGCAGTTTACAAAGACTTGTGTTAATGGGACTGTGAGAGAATTGATTATAGATATCTATGAGTATTGGAAAGTAAAAGACTTATTT
TARGET=169,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5389
PRIMER_LEFT_PENALTY=1.186467
PRIMER_RIGHT_PENALTY=1.352415
PRIMER_LEFT_SEQUENCE=TCGTGTCGTTCAGGTTTTAGTT
PRIMER_RIGHT_SEQUENCE=TCTCTCACAGTCCCATTAACACA
PRIMER_LEFT=16,22
PRIMER_RIGHT=218,23
PRIMER_LEFT_TM=58.814
PRIMER_RIGHT_TM=59.648
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=2.5406
PRIMER_LEFT_1_PENALTY=1.186467
PRIMER_RIGHT_1_PENALTY=1.354152
PRIMER_LEFT_1_SEQUENCE=TCGTGTCGTTCAGGTTTTAGTT
PRIMER_RIGHT_1_SEQUENCE=TCAATTCTCTCACAGTCCCATT
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=223,22
PRIMER_LEFT_1_TM=58.814
PRIMER_RIGHT_1_TM=58.646
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=2.5406
PRIMER_LEFT_2_PENALTY=1.186467
PRIMER_RIGHT_2_PENALTY=1.354152
PRIMER_LEFT_2_SEQUENCE=TCGTGTCGTTCAGGTTTTAGTT
PRIMER_RIGHT_2_SEQUENCE=AATCAATTCTCTCACAGTCCCA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=225,22
PRIMER_LEFT_2_TM=58.814
PRIMER_RIGHT_2_TM=58.646
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=2.7364
PRIMER_LEFT_3_PENALTY=1.383967
PRIMER_RIGHT_3_PENALTY=1.352415
PRIMER_LEFT_3_SEQUENCE=GTCGTGTCGTTCAGGTTTTAGTT
PRIMER_RIGHT_3_SEQUENCE=TCTCTCACAGTCCCATTAACACA
PRIMER_LEFT_3=15,23
PRIMER_RIGHT_3=218,23
PRIMER_LEFT_3_TM=59.616
PRIMER_RIGHT_3_TM=59.648
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=2.7381
PRIMER_LEFT_4_PENALTY=1.383967
PRIMER_RIGHT_4_PENALTY=1.354152
PRIMER_LEFT_4_SEQUENCE=GTCGTGTCGTTCAGGTTTTAGTT
PRIMER_RIGHT_4_SEQUENCE=TCAATTCTCTCACAGTCCCATT
PRIMER_LEFT_4=15,23
PRIMER_RIGHT_4=223,22
PRIMER_LEFT_4_TM=59.616
PRIMER_RIGHT_4_TM=58.646
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_04563 Ps_contig_mira-and-tgicl-ass_franssen_33737
SEQUENCE=GTCGGGGATGGAATGAATCAAAGAAAAAGAAAAGTGATTCCCAGCCTGTGAGTTCTTACAGCGTGTGGGTCCCACTTGACTCGTCTTGCCAGGTCAGTCTTCTCTTCCATCTTCTTCTTCCGTTCTTCCTCCTCCTCCTCCTCCGACATCTTTCACACCATGAAAAACTTCAACCAAAACAACCTCTTCTCTCTTCTCTTCTTCCTTTCCTTCTCTACACTCTCCGTCTTTTCCCACCTCTTCTCTCCTCCCGACAACTACCTCATCAACTGCGGTTCCACCTCCGCCACCACCCTCCTAAACAACCGTCCTTTCACCGGCGACCTCGCCGCCAAACACTCTCCTTCTTTTTCCACCTCCTCACATCCCCTTTTTGTCAAAAACCAAAACCCACTTTCCAATGTGCCACAAATTTACCATACTGCCAGGGTTTTCACCAAACCTACCAAATACTCGTTTCCGATCAAACAAAAAGGTACCCACATGGTTCGTTTCCACTTTCATGCCTTCAATTCCTCGAATCTCGACCTGGGTCGTGCTCAATTTAACATCTTGGTTGATGGGTATGTTGTTTTGAGTAATTTCACTCGTTTTGTTACTGACAGTGAAAGAAACCCTAGGGTAGTTGAGTATCTCGTATGGGTTGATTCTGAAAAGCTTGTGATCGTGTTTGTGCCTAGTAAAGATTCGGAATTGGCGTTTGTCAATGCTATTGAGGTGATTTCTGCACCTACAGACCTTATTCTTGAAACAGCACAGTATTTGAGTTCAGGGAATTTGAAACGTTTTGATGGTTTAAACAAACAAGCTCTTGAAGTTGTTTATAGGGTCACTGTTGGGGGTCCTAAAGTTACACCTTTTAATGATTCCTTGTGGAGGACTTGGGTTCCTGATGAAGAGTTTCTCAAATCAAGTGTGGGGTCTGAGAAGTTTTACTTTGGTGGTAGGATCAATTATCATGTTGGAGGTGCTAGTCGCGAGGTTGGACCGGATAATGTCTATAACACTGCTAGGTTGATTAGAAGTAAGAATGATTCTGTGCCTAATGTTAATATGACTTGGGTGTTTCCAGTTAAGGGTGGATATAAGTACCTGGTTAGGTTGCATTTCTGTGATATTGCTAGCATTTCGGGTGGTTTGCTATATTTCAATGTATATGTGAATGGTTATTTGGTTCTTGAAGATTTGGATATCTCTTATATTACAAGTTCGCTGGCTTCTCCATATTATGTTGACTTTGTTGTCGATGGAGATAGTAGTGTAGGGGCTTTGAGTGTTAGTATAGGTCCTTCAAAGAGCAGTCTTCCGCACGTCATTGATGGAATGTTGAATGCAGTTGAGGTTATGAAGTTGAACAATCAATACAAGAGTCTTGATGGAGATGTTTGTGCTGATTTTGTTCTCAAAAGCCACCGGTCACATGGAAATACGGGTATTTTTCTTACTTTGGTGGCTGCTGTATGCATTGTGTTAAGCCTATCCATAGTAATTCGTAGAAGGATTATTGAGTCACGGGAATCTGTATCATGGTCAAGGTTGCCCGTGAATTCATCTGAGGATAATGTGAAGTGTTAAGACGGCAATGTTTAATCTACAAGTAAATAAGTTATTTGTAGAATATCAGTAAATCTAGCCATCTAGGTGGCTTTGATAAGAAAAGAGAGATGACATGCTGCTGATCAAGCTACGAAGGATGCCAGAGTATGAAATATCGATTGATAACACTTTTTAGGAATTGTTGTAATATATCTAAGTTGTCTTACTTGTTTATATCATAATATTAAGGCATACCTTTTGCCATTACACTATTTTGTTTCAATAATTTTTTGTTTTGTTCTGTTTTTAATATTGTAGCTTTTATCATTGTAACTGTTTGAAGTGTGGAAATGATTATGCAACTTTCATTTTGAGTAGTRGTAATACAAGTGTACTGATGAAAACC
TARGET=127,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04564 Ps_contig_mira-and-tgicl-ass_franssen_33738
SEQUENCE=GTCGGAGGGTCCCGGACAATTGGGGATGGCAGCAGCAGCAGCAACGGCAGTTGTAGGAGGAGTAAGGTGCTGCCCACCCAAATTCAAATCATCATCTTCTCACAAAACAACAATCAATGCTTCCTTCTTTTGGGAACCTAACCCCAAACGCAACACCTATTCATCATCATCATCACACCACCAACAATTCACTTTCCCTCTCACCACTCAATCATCATCATCATCCCCTCTCACCACCAACACCATTACTATCTCTTTTGTATCTTCAATCTCACAAATCTCATCCACTCAATGGGACGCTTGTGCCCTCGACGCTACCGGCCACCACAACTACAATCCATTCCTCTCCCACGCTTTCCTATCCACCTTAGAACATTCCCATTCCGCCGTCAAAGAAAAAGGATGGACCCCACACCACATCATTGCTCAAGATCAATCCAATCAAATCCTCGCCGTTGTTCCTCTTTATCTCAAAACTCATTCCTATGGTGAATTCGTTTTCGATCATTCTTGGGCTAATGCTTATTACAATTATGGTTCCTCTTATTACCCTAAGTTACAATCGTGCGTTCCTTTTACTCCAGTCACTGGTCCAAGGATCCTACTTCGTGATACTTCTATTAAGAATCATGTTTTTGATTTTATTCTATCTGCAATCAAGGATCTTACTGCCATGTCTCACCTTTCATCGTTTCATGTTACTTTTCCCTCTGAAAATGAGTGGCACAAGTTCACTCAGAAAGGCTTTCTCCCCAGGATTGGTATGCAGTATCACTGGACCAACCGCAACTACAAAAATTTTGATGAATTCTTGATGGACTTGAAGCATAATAAAAGAAAAAAGATACGTCAAGAGCGCAAGAAGGTTGCAGCGCAAAACTTGGTTATGAAACGGCTCCGGGGTTATGAAATTAAGGCAAAGCACTGGGATTCCTTCTACACCTTTTACAGGAACACAACTGATAACAAATGGGGTACCCCTTTCCTGACAAGGGAATTCTTTCACGAGATGGGATCAAAAATGGGAGATCAGGTACTACTTATTGTTGCTGAAGATGGGGGTGAACTAGTTGGGGGTGCCCTAAACCTTATCGGAGGAGATACTTTGTTTGGGCGTCTATGGGGGTGTCAGCCGGAAACTTACTATCCATTTTTGCATTTTGAAGCCTGCTATTATCAGGCAATTGAAGCAGCAATTGAACTGAATCTTAAAACAGTAGAAGCAGGAGCTCAGGGTGAACATAAGATTGAGCGTGGGTATCTTCCTGTGACAACATATAGCTGCCATTACCTTATTGATCAAGAATTTAGCAAAGCTATACAAGATTTTTTAGTTCGTGAAACTTCTCAGGTGAAGATGGTTATGAAACTCTTGCATGATTCTGGTCCTTTCAAGGAAGGTGTCTTTTAGAAAATATTCATCGGCATTGATAACTACATTTGTAAATACACATTTGGTTAATTTTGTTAGAAATGTATGTACAGTTGAATCATTGGCCATTAATTAATATTCGATGTCTTAGGAATGTATGTACAGTTGAATCAGCTGCCATTCATTTATGTTTTAAATTAATTTGGATATGAGTTTTCATGTCAAT
TARGET=29,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04565 Ps_contig_mira-and-tgicl-ass_franssen_33738
SEQUENCE=GTCGGAGGGTCCCGGACAATTGGGGATGGCAGCAGCAGCAGCAACGGCAGTTGTAGGAGGAGTAAGGTGCTGCCCACCCAAATTCAAATCATCATCTTCTCACAAAACAACAATCAATGCTTCCTTCTTTTGGGAACCTAACCCCAAACGCAACACCTATTCATCATCATCATCACACCACCAACAATTCACTTTCCCTCTCACCACTCAATCATCATCATCATCCCCTCTCACCACCAACACCATTACTATCTCTTTTGTATCTTCAATCTCACAAATCTCATCCACTCAATGGGACGCTTGTGCCCTCGACGCTACCGGCCACCACAACTACAATCCATTCCTCTCCCACGCTTTCCTATCCACCTTAGAACATTCCCATTCCGCCGTCAAAGAAAAAGGATGGACCCCACACCACATCATTGCTCAAGATCAATCCAATCAAATCCTCGCCGTTGTTCCTCTTTATCTCAAAACTCATTCCTATGGTGAATTCGTTTTCGATCATTCTTGGGCTAATGCTTATTACAATTATGGTTCCTCTTATTACCCTAAGTTACAATCGTGCGTTCCTTTTACTCCAGTCACTGGTCCAAGGATCCTACTTCGTGATACTTCTATTAAGAATCATGTTTTTGATTTTATTCTATCTGCAATCAAGGATCTTACTGCCATGTCTCACCTTTCATCGTTTCATGTTACTTTTCCCTCTGAAAATGAGTGGCACAAGTTCACTCAGAAAGGCTTTCTCCCCAGGATTGGTATGCAGTATCACTGGACCAACCGCAACTACAAAAATTTTGATGAATTCTTGATGGACTTGAAGCATAATAAAAGAAAAAAGATACGTCAAGAGCGCAAGAAGGTTGCAGCGCAAAACTTGGTTATGAAACGGCTCCGGGGTTATGAAATTAAGGCAAAGCACTGGGATTCCTTCTACACCTTTTACAGGAACACAACTGATAACAAATGGGGTACCCCTTTCCTGACAAGGGAATTCTTTCACGAGATGGGATCAAAAATGGGAGATCAGGTACTACTTATTGTTGCTGAAGATGGGGGTGAACTAGTTGGGGGTGCCCTAAACCTTATCGGAGGAGATACTTTGTTTGGGCGTCTATGGGGGTGTCAGCCGGAAACTTACTATCCATTTTTGCATTTTGAAGCCTGCTATTATCAGGCAATTGAAGCAGCAATTGAACTGAATCTTAAAACAGTAGAAGCAGGAGCTCAGGGTGAACATAAGATTGAGCGTGGGTATCTTCCTGTGACAACATATAGCTGCCATTACCTTATTGATCAAGAATTTAGCAAAGCTATACAAGATTTTTTAGTTCGTGAAACTTCTCAGGTGAAGATGGTTATGAAACTCTTGCATGATTCTGGTCCTTTCAAGGAAGGTGTCTTTTAGAAAATATTCATCGGCATTGATAACTACATTTGTAAATACACATTTGGTTAATTTTGTTAGAAATGTATGTACAGTTGAATCATTGGCCATTAATTAATATTCGATGTCTTAGGAATGTATGTACAGTTGAATCAGCTGCCATTCATTTATGTTTTAAATTAATTTGGATATGAGTTTTCATGTCAAT
TARGET=161,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2086
PRIMER_LEFT_PENALTY=0.185055
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=ACGGCAGTTGTAGGAGGAGTAA
PRIMER_RIGHT_SEQUENCE=GAGAGGGAAAGTGAATTGTTGG
PRIMER_LEFT=43,22
PRIMER_RIGHT=201,22
PRIMER_LEFT_TM=60.185
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=159
PRIMER_PAIR_PENALTY_1=0.2086
PRIMER_LEFT_1_PENALTY=0.185055
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=AACGGCAGTTGTAGGAGGAGTA
PRIMER_RIGHT_1_SEQUENCE=GAGAGGGAAAGTGAATTGTTGG
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=201,22
PRIMER_LEFT_1_TM=60.185
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.2952
PRIMER_LEFT_2_PENALTY=0.185055
PRIMER_RIGHT_2_PENALTY=0.110131
PRIMER_LEFT_2_SEQUENCE=ACGGCAGTTGTAGGAGGAGTAA
PRIMER_RIGHT_2_SEQUENCE=GATTGAGTGGTGAGAGGGAAAG
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=60.185
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.2952
PRIMER_LEFT_3_PENALTY=0.185055
PRIMER_RIGHT_3_PENALTY=0.110131
PRIMER_LEFT_3_SEQUENCE=AACGGCAGTTGTAGGAGGAGTA
PRIMER_RIGHT_3_SEQUENCE=GATTGAGTGGTGAGAGGGAAAG
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=212,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.3310
PRIMER_LEFT_4_PENALTY=0.307443
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=CGGCAGTTGTAGGAGGAGTAAG
PRIMER_RIGHT_4_SEQUENCE=GAGAGGGAAAGTGAATTGTTGG
PRIMER_LEFT_4=44,22
PRIMER_RIGHT_4=201,22
PRIMER_LEFT_4_TM=60.307
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_04566 Ps_contig_mira-and-tgicl-ass_franssen_33738
SEQUENCE=GTCGGAGGGTCCCGGACAATTGGGGATGGCAGCAGCAGCAGCAACGGCAGTTGTAGGAGGAGTAAGGTGCTGCCCACCCAAATTCAAATCATCATCTTCTCACAAAACAACAATCAATGCTTCCTTCTTTTGGGAACCTAACCCCAAACGCAACACCTATTCATCATCATCATCACACCACCAACAATTCACTTTCCCTCTCACCACTCAATCATCATCATCATCCCCTCTCACCACCAACACCATTACTATCTCTTTTGTATCTTCAATCTCACAAATCTCATCCACTCAATGGGACGCTTGTGCCCTCGACGCTACCGGCCACCACAACTACAATCCATTCCTCTCCCACGCTTTCCTATCCACCTTAGAACATTCCCATTCCGCCGTCAAAGAAAAAGGATGGACCCCACACCACATCATTGCTCAAGATCAATCCAATCAAATCCTCGCCGTTGTTCCTCTTTATCTCAAAACTCATTCCTATGGTGAATTCGTTTTCGATCATTCTTGGGCTAATGCTTATTACAATTATGGTTCCTCTTATTACCCTAAGTTACAATCGTGCGTTCCTTTTACTCCAGTCACTGGTCCAAGGATCCTACTTCGTGATACTTCTATTAAGAATCATGTTTTTGATTTTATTCTATCTGCAATCAAGGATCTTACTGCCATGTCTCACCTTTCATCGTTTCATGTTACTTTTCCCTCTGAAAATGAGTGGCACAAGTTCACTCAGAAAGGCTTTCTCCCCAGGATTGGTATGCAGTATCACTGGACCAACCGCAACTACAAAAATTTTGATGAATTCTTGATGGACTTGAAGCATAATAAAAGAAAAAAGATACGTCAAGAGCGCAAGAAGGTTGCAGCGCAAAACTTGGTTATGAAACGGCTCCGGGGTTATGAAATTAAGGCAAAGCACTGGGATTCCTTCTACACCTTTTACAGGAACACAACTGATAACAAATGGGGTACCCCTTTCCTGACAAGGGAATTCTTTCACGAGATGGGATCAAAAATGGGAGATCAGGTACTACTTATTGTTGCTGAAGATGGGGGTGAACTAGTTGGGGGTGCCCTAAACCTTATCGGAGGAGATACTTTGTTTGGGCGTCTATGGGGGTGTCAGCCGGAAACTTACTATCCATTTTTGCATTTTGAAGCCTGCTATTATCAGGCAATTGAAGCAGCAATTGAACTGAATCTTAAAACAGTAGAAGCAGGAGCTCAGGGTGAACATAAGATTGAGCGTGGGTATCTTCCTGTGACAACATATAGCTGCCATTACCTTATTGATCAAGAATTTAGCAAAGCTATACAAGATTTTTTAGTTCGTGAAACTTCTCAGGTGAAGATGGTTATGAAACTCTTGCATGATTCTGGTCCTTTCAAGGAAGGTGTCTTTTAGAAAATATTCATCGGCATTGATAACTACATTTGTAAATACACATTTGGTTAATTTTGTTAGAAATGTATGTACAGTTGAATCATTGGCCATTAATTAATATTCGATGTCTTAGGAATGTATGTACAGTTGAATCAGCTGCCATTCATTTATGTTTTAAATTAATTTGGATATGAGTTTTCATGTCAAT
TARGET=211,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2215
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.197963
PRIMER_LEFT_SEQUENCE=CCAACAATTCACTTTCCCTCTC
PRIMER_RIGHT_SEQUENCE=GTCCCATTGAGTGGATGAGATT
PRIMER_LEFT=180,22
PRIMER_RIGHT=297,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.198
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=118
PRIMER_PAIR_PENALTY_1=0.3197
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.296177
PRIMER_LEFT_1_SEQUENCE=CCAACAATTCACTTTCCCTCTC
PRIMER_RIGHT_1_SEQUENCE=AATGGGAATGTTCTAAGGTGGA
PRIMER_LEFT_1=180,22
PRIMER_RIGHT_1=382,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.704
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.3197
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.296177
PRIMER_LEFT_2_SEQUENCE=CCAACAATTCACTTTCCCTCTC
PRIMER_RIGHT_2_SEQUENCE=GGAATGGGAATGTTCTAAGGTG
PRIMER_LEFT_2=180,22
PRIMER_RIGHT_2=384,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=59.704
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.3456
PRIMER_LEFT_3_PENALTY=0.203324
PRIMER_RIGHT_3_PENALTY=0.142283
PRIMER_LEFT_3_SEQUENCE=CACACCACCAACAATTCACTTT
PRIMER_RIGHT_3_SEQUENCE=CTAAGGTGGATAGGAAAGCGTG
PRIMER_LEFT_3=173,22
PRIMER_RIGHT_3=370,22
PRIMER_LEFT_3_TM=59.797
PRIMER_RIGHT_3_TM=60.142
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=0.3830
PRIMER_LEFT_4_PENALTY=0.185055
PRIMER_RIGHT_4_PENALTY=0.197963
PRIMER_LEFT_4_SEQUENCE=ACGGCAGTTGTAGGAGGAGTAA
PRIMER_RIGHT_4_SEQUENCE=GTCCCATTGAGTGGATGAGATT
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=60.185
PRIMER_RIGHT_4_TM=60.198
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_04567 Ps_contig_mira-and-tgicl-ass_franssen_33742
SEQUENCE=GCAAAGACTTTAAAATATAATTGCTGCTTTTGAAAATTAACATAATGTATGTATGTTATGAATTTATGTTCCATCCTGTTCAATGAGAATTTCAAACTTATTGCAATAACTGAGGTAGTTGGTAGTAGCTTTGTATTCCAGTTTCATCTATTTTTACTCTTGCATCCATTATGATACATATTTTGTTTCTCTATCTTGCTTCCGTAGGGATCTACATGCACCTTCACAGAATCTATATTACGCAACTGTGGTTTTCGTACTGGACTTTTTACATCTCCTCACCTCATTGATATCCGAGAAAGATTTCGTTTAAATGGTATCGAAATCTGTCAAGAGAAATTTTTAGC
TARGET=45,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04568 Ps_contig_mira-and-tgicl-ass_franssen_33747
SEQUENCE=AATTATGAGTAGAANATAGCTAAACACTCCAACTCCATCATCATCATCATCGTGCAAAGATATGGCGGATTCTATTTCATTCTTCGCCTCTCTTCCTGAAGATGTCAACTTCAAAATTGCTTCACTTCTTCAGGTGCGGGATTTGTGTGCTTTGGGTTGCTCTTCAAAGTTCTGGAAACAAGTCTGTTTCTCTGATTCCATTTGGCATCATCTTCTAACTAACAGATGGCCCTTATTCCGTTCACCTCTCTCTCCCAATCTCAAGACATGGAGAAGATTGTATTTCGAGAGGCACATCGATTTGGGACTTAGAGCAGGCTCTGTCGAGAGGTTTTTGAAAGGTTGTTCGCGTAATGAATCACTCGAGGTTGACGACTATCTGCAAGCCGTTGAAATTGTGAATGGTGCAAGGTTTGGTTTTGAAGACATTCAAAGGCTCCTGTTTAAACCTGAAATGAACGTGTTGGTTAACTTGGTTGGTGTGCATTATTGCATAACAAACCTTGGGATTCCGGGTGATAATCTTGTAAAAGTCCTTCGAACTTGCGAGATCTCAGATCGACATGTATGCGTCAAGTGGTGGAAACTTGGTAGGTGGATACATGGATACCGCGGTAGAGATGAGTTACTTTTTCGTTGGATTTCTTTGGAAAATTTGGCAACAGAAGAAGATGAGACTGTTTTGAGAGTGCTTCGTCGAGGCACTGTTCTTGAGGTTTTACGCGTTCAGATCTCAGCTGTTGATCATAAATCAATACCTTGGTCCTATCAGGATAATCAGTGATTGGAATTGCTGCAGGGAAATGAAATCAACTAAAAATGAACTATAAGTATCAATATTTATCAGCATTAGTATGTGTATAATAATAGTATATGTCAATATCTATTACTATGTGTATAGTTAAACATGTGTTGTATGTTAATAAAAACAGGGCAATAATAAATAATAATGCTTATTATCTTACTTACC
TARGET=36,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.5109
PRIMER_LEFT_PENALTY=4.461188
PRIMER_RIGHT_PENALTY=0.049672
PRIMER_LEFT_SEQUENCE=GAANATAGCTAAACACTCCAACTCC
PRIMER_RIGHT_SEQUENCE=TCTTGAGATTGGGAGAGAGAGG
PRIMER_LEFT=11,25
PRIMER_RIGHT=265,22
PRIMER_LEFT_TM=58.539
PRIMER_RIGHT_TM=59.950
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=255
PRIMER_PAIR_PENALTY_1=4.6439
PRIMER_LEFT_1_PENALTY=4.461188
PRIMER_RIGHT_1_PENALTY=0.182682
PRIMER_LEFT_1_SEQUENCE=GAANATAGCTAAACACTCCAACTCC
PRIMER_RIGHT_1_SEQUENCE=GAGATTGGGAGAGAGAGGTGAA
PRIMER_LEFT_1=11,25
PRIMER_RIGHT_1=261,22
PRIMER_LEFT_1_TM=58.539
PRIMER_RIGHT_1_TM=59.817
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=4.7360
PRIMER_LEFT_2_PENALTY=4.461188
PRIMER_RIGHT_2_PENALTY=0.274768
PRIMER_LEFT_2_SEQUENCE=GAANATAGCTAAACACTCCAACTCC
PRIMER_RIGHT_2_SEQUENCE=AGAACTTTGAAGAGCAACCCAA
PRIMER_LEFT_2=11,25
PRIMER_RIGHT_2=172,22
PRIMER_LEFT_2_TM=58.539
PRIMER_RIGHT_2_TM=60.275
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=4.7484
PRIMER_LEFT_3_PENALTY=4.461188
PRIMER_RIGHT_3_PENALTY=0.287182
PRIMER_LEFT_3_SEQUENCE=GAANATAGCTAAACACTCCAACTCC
PRIMER_RIGHT_3_SEQUENCE=GGAGAGAGAGGTGAACGGAATA
PRIMER_LEFT_3=11,25
PRIMER_RIGHT_3=254,22
PRIMER_LEFT_3_TM=58.539
PRIMER_RIGHT_3_TM=59.713
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=4.9408
PRIMER_LEFT_4_PENALTY=4.461188
PRIMER_RIGHT_4_PENALTY=0.479611
PRIMER_LEFT_4_SEQUENCE=GAANATAGCTAAACACTCCAACTCC
PRIMER_RIGHT_4_SEQUENCE=ATCGATGTGCCTCTCGAAATAC
PRIMER_LEFT_4=11,25
PRIMER_RIGHT_4=300,22
PRIMER_LEFT_4_TM=58.539
PRIMER_RIGHT_4_TM=60.480
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_04569 Ps_contig_mira-and-tgicl-ass_franssen_33754
SEQUENCE=CCCAACCCAATTACGTCAGGTTCANCCGTTTCCTATGCTTCAGAAGTGAAGAGAATTACGGCTCAACCAAAGGATGAGATATCTTCTACTGAGGAATCACAGAGCTCTGCTGTTAGAACTGTAGCCTTATGGGTTTTCGCAGCAATAGCGTTTGGTGTTGGCTTGGGTTTTAAAGAAGGCTATGAAAAGGCGTCTGAATTTTTTGCTGGATACATATTGGAGCAAAGTCTCTCCGTGGATAATCTCTTTGTCTTTGTTCTAATATTCAAATACTTCAAAGTGCCAGCTTTGTATCAGAATCGTGTACTTTCCTATGGTATTGCCGGTGCAGTTGTTTTTCGCCTGACAATAATACTTCTTGGAACAGCCACCATTCAGAGGTTTGAGGCAGTAAACCTTCTATTGGCGGCAATATTACTCTTCTCATCATTTAAGCTATTTGCTAGTGAAGACGAAGAGTCAGACTTATCTGATAACTTTTTGGTGAAGACATGCCAGAAACTTATCCCTGTAACAACAAATTATGATGGAAACAAGTTCATAACAAAGCAGGATGGGATATGGATGGCCACACCTTTGCTTCTTACAGTAGCAGTTATTGAACTCAGTGACATTGCATTTGCTGTGGACTCAATACCTGCGGTTTTTGGTGTAACACGGGATCCATTTATAGTGTTTTCTTCTAATCTTTTTGCCATTCTGGGCTTGAGGTCACTTTACTTGATAATTTCGGAGGGTATGTCAGAATTGAAGTACTTACAGCCATCCATTGCTGTTGTTCTTGGATTTATCGGGTGTAAGATGATCTTGGACTATTTTGGAATCCATGTCTCAACAGAAGCATCGCTCGGTTTCGTAGCCTCTAGTCTTTCCATTGGAGTGATATTAAGTCTGGCTAACAAATCCGACTAAGCTTATAGACCAACTCAGAATGAGTTGAGCTCTGCATGATTGCTTCTATTTTACAGCAATCCAATTCTCCCCCACCCCAAATAAAAGATAAATCCATATATTCTTCCTTTATCATCTTGTATACTTGGTAAGAAATATTTATTTATTTGTAGCCTCTGATATGGCTCTAGTTCATGTTCAAAGTTAAATCTTAATAGCAAATTGTCTATAC
TARGET=1048,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1383
PRIMER_LEFT_PENALTY=0.029253
PRIMER_RIGHT_PENALTY=1.109069
PRIMER_LEFT_SEQUENCE=TTTGGAATCCATGTCTCAACAG
PRIMER_RIGHT_SEQUENCE=TGAACTAGAGCCATATCAGAGGC
PRIMER_LEFT=816,22
PRIMER_RIGHT=1085,23
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=59.891
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=270
PRIMER_PAIR_PENALTY_1=1.3688
PRIMER_LEFT_1_PENALTY=0.259704
PRIMER_RIGHT_1_PENALTY=1.109069
PRIMER_LEFT_1_SEQUENCE=AATCCATGTCTCAACAGAAGCA
PRIMER_RIGHT_1_SEQUENCE=TGAACTAGAGCCATATCAGAGGC
PRIMER_LEFT_1=821,22
PRIMER_RIGHT_1=1085,23
PRIMER_LEFT_1_TM=59.740
PRIMER_RIGHT_1_TM=59.891
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=1.4212
PRIMER_LEFT_2_PENALTY=0.312177
PRIMER_RIGHT_2_PENALTY=1.109069
PRIMER_LEFT_2_SEQUENCE=TTTACAGCAATCCAATTCTCCC
PRIMER_RIGHT_2_SEQUENCE=TGAACTAGAGCCATATCAGAGGC
PRIMER_LEFT_2=961,22
PRIMER_RIGHT_2=1085,23
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=59.891
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=1.4509
PRIMER_LEFT_3_PENALTY=0.341875
PRIMER_RIGHT_3_PENALTY=1.109069
PRIMER_LEFT_3_SEQUENCE=AAGTCTGGCTAACAAATCCGAC
PRIMER_RIGHT_3_SEQUENCE=TGAACTAGAGCCATATCAGAGGC
PRIMER_LEFT_3=887,22
PRIMER_RIGHT_3=1085,23
PRIMER_LEFT_3_TM=59.658
PRIMER_RIGHT_3_TM=59.891
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=199
PRIMER_PAIR_PENALTY_4=1.6042
PRIMER_LEFT_4_PENALTY=0.495173
PRIMER_RIGHT_4_PENALTY=1.109069
PRIMER_LEFT_4_SEQUENCE=TGGAATCCATGTCTCAACAGAA
PRIMER_RIGHT_4_SEQUENCE=TGAACTAGAGCCATATCAGAGGC
PRIMER_LEFT_4=818,22
PRIMER_RIGHT_4=1085,23
PRIMER_LEFT_4_TM=60.495
PRIMER_RIGHT_4_TM=59.891
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_04570 Ps_contig_mira-and-tgicl-ass_franssen_33756
SEQUENCE=AGTATTATTACTATTATTATTATTATAGTTTTCTTCTTTCATTCTCTCACTCTTTTTCTATCTCTAAAGTCTAAAACCTTTCTTTTCAATATTCTCCAACTTATCATTATTTGATTTGCTTGTAAATTGCAAAACATAGAGAAAAAGAATGAAGAAATTTGTACTCAAGTTAGAATTGCATGATGACAAAGACAAGAAGAAGGCTCTCAAAACTGTCTCAACTCTTTCAGGGATTGATGCCATTTCAATGGACATGAAAGACAAGAAAATGACAGTGATAGGAACAGTAGATCCTGTAAATGTGGTGAGCAAATTAAGAAAATTCTGGCACACAGAAATAATAGCAGTGGGACCAGCAAAAGAGCCAGAGAAGAAAGAAGAAGCAAAGAAGGAAGGGGAGAAGAAAGAAGAAGGGAAGAAAGAAGATGGAAAGAAAGAAGAAGGAAAGAAAGAAGAAGAGAAGAAAAAAGAAGCAGCTCCAGCAGCAGTAGATCCTGTTCAAGAATGGGTTAGGAATTATAGGGCATATAATCCATATATGACAACACATTACCATGTTCAAAGCATGGAAGAGAATCCTAATGCATGTGTCATTTGTTAAATAATAACAGTTTAATTATCTTTTCTCTCATAGCCTCATATTATCATAGGTGGCCACATGCAATTCAACAATGTAGGAGCTACTTGTAAATAATATATGGTTATGCCTTGTTGAGACTTTATTAATATGTGTAGAGAGAAAGAAATTAAAATTTTGGAAATTTTTAGGTTTNRAAAAAGG
TARGET=12,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04571 Ps_contig_mira-and-tgicl-ass_franssen_33758
SEQUENCE=CCACATAGTATAATAANATATACATATGAATGACAAATTCCATTTTCAACCTTTGACAATTACACACGCTATACCCTTCACCTTCCACGAACCTTCATTCAACTTTACCAATCTTTATTTAACTTGTATTGTTTTTTCACAATATTATCACCTACTTAATTTTCTCAAACAATTTCTTAGAGTAACCATGAACATGAACATGATGAACAACGATAACAATGAACAATCCTTCTGGCAATTCAGTGACCAACTAAGACACCAAGCATCAAACTTAGCAAACCTGTCACTAAATGATTCAATTTGGAGCAACAACAAAAGACCTGATCAAAGGAGAAACTTTGATGTCAAAACCAATGGAGTTTCTGATATTAACAACTCAAAGCCTAGTGATTTCAATGATGGGTGGAAGATGATGAACAATTCTAATGGATCAGTTTTTTCCATGCCGCATAATAATTCATTTGCTGGATTTAATAAAGGGCTTTATTCTTCTCCTTATCTTAACAACAACAACAACAACCTTAACCACAACAACGTTAACAACATCAATCTTAATGGTTACAAATCAGGTTTTAAGGTTGGTGATGAGTTTCAATTACCTAACAAAGGTGTTAAGAAGAATCTCAACAACAACAACAGTAGCAACAAGAAGCATGGAGATAACACTGATGTCGGTAAAACTTCTGTGGACAAGAAGTTCAAAACTCTGCCACCGTCTGAATCACTGCCTAGAAATGAAACTATTGGTGGGTATATCTTTGTTTGCAACAATGATACCATGGCGGAGAATCTCAAGAGGCAGATCTTTGGTCTGCCTCCAAGATACAGAGATTCAGTTAGAACAATAACTCCAGGGTTACCCCTTTTTCTGTACAACTATTCCACCCACCAACTCCATGGTATTTTTGAGGCTGCAAGTTTTGGAGGAAGTAATATTGATCCAACAGCTTGGGAGGATAAGAAATGTCCCGGCGAATCTCGTTTCCCTGCTCAGGTACAGGTGATCAATAGAAAAGTTTGTGAGCCACTAGAAGAGGATTCCTTTAGACCAATTCTTCACCACTACGACGGTCCTAAGTTTCGTCTTGAACTGAGTGTGTCTGAGGCACTGTCTCTTCTGGATATTTTTGCAGATCAGAATAGTTTCAATGATATTTTCAAGGCTATACCAGCATAAAATGGTAGAAATAAAAGAGAATACAAGAACCTTAAGTTATGAAGAAAAATAGAAAATGAATACTGTGGACTTGTAAAGAAGGTCCTAATATATGACAATGAATATGGTGGAGGAAGATTGAATATTCAGCCTATATATGTATAAGTATATGAGTTTTGATGTTATTTGTATATAGAGGCTGTTATGCCTAATTTCCTCCCTTTTTATTGTGAGTGTATATTAACATATAAAAGAATACAATAAGAATGAACTAATTTATCTTATAGCCAATTATTATGTACTATAATTTATCTTCTTAGCTTGTTGATTAACCTAGCCAAAAAGCATATGTTCACATGCGTTTTTTTTCCAACGTAATACTCATGATAGTGTATCTGGGTCTTTGGATTCTCGTTTAGAGCATTTAATTTATTTTGGTGGTTCCTTAC
TARGET=503,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7554
PRIMER_LEFT_PENALTY=0.314970
PRIMER_RIGHT_PENALTY=0.440473
PRIMER_LEFT_SEQUENCE=TAGTGATTTCAATGATGGGTGG
PRIMER_RIGHT_SEQUENCE=TCCATGCTTCTTGTTGCTACTG
PRIMER_LEFT=384,22
PRIMER_RIGHT=657,22
PRIMER_LEFT_TM=59.685
PRIMER_RIGHT_TM=60.440
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.8676
PRIMER_LEFT_1_PENALTY=0.314970
PRIMER_RIGHT_1_PENALTY=0.552581
PRIMER_LEFT_1_SEQUENCE=TAGTGATTTCAATGATGGGTGG
PRIMER_RIGHT_1_SEQUENCE=CCATGCTTCTTGTTGCTACTGT
PRIMER_LEFT_1=384,22
PRIMER_RIGHT_1=656,22
PRIMER_LEFT_1_TM=59.685
PRIMER_RIGHT_1_TM=59.447
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.9496
PRIMER_LEFT_2_PENALTY=0.314970
PRIMER_RIGHT_2_PENALTY=0.634599
PRIMER_LEFT_2_SEQUENCE=TAGTGATTTCAATGATGGGTGG
PRIMER_RIGHT_2_SEQUENCE=TTATCTCCATGCTTCTTGTTGC
PRIMER_LEFT_2=384,22
PRIMER_RIGHT_2=662,22
PRIMER_LEFT_2_TM=59.685
PRIMER_RIGHT_2_TM=59.365
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=1.3152
PRIMER_LEFT_3_PENALTY=0.314970
PRIMER_RIGHT_3_PENALTY=1.000269
PRIMER_LEFT_3_SEQUENCE=TAGTGATTTCAATGATGGGTGG
PRIMER_RIGHT_3_SEQUENCE=TATCTCCATGCTTCTTGTTGCT
PRIMER_LEFT_3=384,22
PRIMER_RIGHT_3=661,22
PRIMER_LEFT_3_TM=59.685
PRIMER_RIGHT_3_TM=59.000
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=1.3152
PRIMER_LEFT_4_PENALTY=0.314970
PRIMER_RIGHT_4_PENALTY=1.000269
PRIMER_LEFT_4_SEQUENCE=TAGTGATTTCAATGATGGGTGG
PRIMER_RIGHT_4_SEQUENCE=ATCTCCATGCTTCTTGTTGCTA
PRIMER_LEFT_4=384,22
PRIMER_RIGHT_4=660,22
PRIMER_LEFT_4_TM=59.685
PRIMER_RIGHT_4_TM=59.000
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=277
=
PRIMER_SEQUENCE_ID=SSR_04572 Ps_contig_mira-and-tgicl-ass_franssen_33758
SEQUENCE=CCACATAGTATAATAANATATACATATGAATGACAAATTCCATTTTCAACCTTTGACAATTACACACGCTATACCCTTCACCTTCCACGAACCTTCATTCAACTTTACCAATCTTTATTTAACTTGTATTGTTTTTTCACAATATTATCACCTACTTAATTTTCTCAAACAATTTCTTAGAGTAACCATGAACATGAACATGATGAACAACGATAACAATGAACAATCCTTCTGGCAATTCAGTGACCAACTAAGACACCAAGCATCAAACTTAGCAAACCTGTCACTAAATGATTCAATTTGGAGCAACAACAAAAGACCTGATCAAAGGAGAAACTTTGATGTCAAAACCAATGGAGTTTCTGATATTAACAACTCAAAGCCTAGTGATTTCAATGATGGGTGGAAGATGATGAACAATTCTAATGGATCAGTTTTTTCCATGCCGCATAATAATTCATTTGCTGGATTTAATAAAGGGCTTTATTCTTCTCCTTATCTTAACAACAACAACAACAACCTTAACCACAACAACGTTAACAACATCAATCTTAATGGTTACAAATCAGGTTTTAAGGTTGGTGATGAGTTTCAATTACCTAACAAAGGTGTTAAGAAGAATCTCAACAACAACAACAGTAGCAACAAGAAGCATGGAGATAACACTGATGTCGGTAAAACTTCTGTGGACAAGAAGTTCAAAACTCTGCCACCGTCTGAATCACTGCCTAGAAATGAAACTATTGGTGGGTATATCTTTGTTTGCAACAATGATACCATGGCGGAGAATCTCAAGAGGCAGATCTTTGGTCTGCCTCCAAGATACAGAGATTCAGTTAGAACAATAACTCCAGGGTTACCCCTTTTTCTGTACAACTATTCCACCCACCAACTCCATGGTATTTTTGAGGCTGCAAGTTTTGGAGGAAGTAATATTGATCCAACAGCTTGGGAGGATAAGAAATGTCCCGGCGAATCTCGTTTCCCTGCTCAGGTACAGGTGATCAATAGAAAAGTTTGTGAGCCACTAGAAGAGGATTCCTTTAGACCAATTCTTCACCACTACGACGGTCCTAAGTTTCGTCTTGAACTGAGTGTGTCTGAGGCACTGTCTCTTCTGGATATTTTTGCAGATCAGAATAGTTTCAATGATATTTTCAAGGCTATACCAGCATAAAATGGTAGAAATAAAAGAGAATACAAGAACCTTAAGTTATGAAGAAAAATAGAAAATGAATACTGTGGACTTGTAAAGAAGGTCCTAATATATGACAATGAATATGGTGGAGGAAGATTGAATATTCAGCCTATATATGTATAAGTATATGAGTTTTGATGTTATTTGTATATAGAGGCTGTTATGCCTAATTTCCTCCCTTTTTATTGTGAGTGTATATTAACATATAAAAGAATACAATAAGAATGAACTAATTTATCTTATAGCCAATTATTATGTACTATAATTTATCTTCTTAGCTTGTTGATTAACCTAGCCAAAAAGCATATGTTCACATGCGTTTTTTTTCCAACGTAATACTCATGATAGTGTATCTGGGTCTTTGGATTCTCGTTTAGAGCATTTAATTTATTTTGGTGGTTCCTTAC
TARGET=625,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9496
PRIMER_LEFT_PENALTY=0.314970
PRIMER_RIGHT_PENALTY=0.634599
PRIMER_LEFT_SEQUENCE=TAGTGATTTCAATGATGGGTGG
PRIMER_RIGHT_SEQUENCE=TTATCTCCATGCTTCTTGTTGC
PRIMER_LEFT=384,22
PRIMER_RIGHT=662,22
PRIMER_LEFT_TM=59.685
PRIMER_RIGHT_TM=59.365
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=1.1366
PRIMER_LEFT_1_PENALTY=1.006228
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=TCAGGTTTTAAGGTTGGTGATG
PRIMER_RIGHT_1_SEQUENCE=GTATCTTGGAGGCAGACCAAAG
PRIMER_LEFT_1=565,22
PRIMER_RIGHT_1=825,22
PRIMER_LEFT_1_TM=58.994
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=1.1366
PRIMER_LEFT_2_PENALTY=1.006228
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=CAGGTTTTAAGGTTGGTGATGA
PRIMER_RIGHT_2_SEQUENCE=GTATCTTGGAGGCAGACCAAAG
PRIMER_LEFT_2=566,22
PRIMER_RIGHT_2=825,22
PRIMER_LEFT_2_TM=58.994
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=1.1578
PRIMER_LEFT_3_PENALTY=1.006228
PRIMER_RIGHT_3_PENALTY=0.151533
PRIMER_LEFT_3_SEQUENCE=TCAGGTTTTAAGGTTGGTGATG
PRIMER_RIGHT_3_SEQUENCE=CCAAAGATCTGCCTCTTGAGAT
PRIMER_LEFT_3=565,22
PRIMER_RIGHT_3=809,22
PRIMER_LEFT_3_TM=58.994
PRIMER_RIGHT_3_TM=59.848
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=1.1578
PRIMER_LEFT_4_PENALTY=1.006228
PRIMER_RIGHT_4_PENALTY=0.151533
PRIMER_LEFT_4_SEQUENCE=CAGGTTTTAAGGTTGGTGATGA
PRIMER_RIGHT_4_SEQUENCE=CCAAAGATCTGCCTCTTGAGAT
PRIMER_LEFT_4=566,22
PRIMER_RIGHT_4=809,22
PRIMER_LEFT_4_TM=58.994
PRIMER_RIGHT_4_TM=59.848
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=244
=
PRIMER_SEQUENCE_ID=SSR_04573 Ps_contig_mira-and-tgicl-ass_franssen_33760
SEQUENCE=CCCGACCGAACAGAACACACCCTCCTTCCTTCACCACCTCTCGCTAACTTGCGCCTCATTCTTAACCTACGCACTCTCCTCTTTCTTTCCATAACCTTCTATTAACCCTTCTCTCTCTTTCTCTCTCTCTCTATTATTCTGTTGCGAGATTCATATCACCACCCCCATTGGATTAGGTTCCGGTGTTGGGGGTATTAGGGTTAGGGTTTCTGAGAGATGTGGGATGCTTCAGCCATTGATGAATCTTCTCTCTCCATGTTGGAAGCCGTTCGGGCACGGTGACGATTCTTCAGCAGGCATTGTTGGAAGAGAAGGCAAAGACGGATTGCTTTGGTTTCGTGACATCGGAAGATATGGCTCCGGTGAATTCTCTATGGCTGTTGTTCAGGCCAATCAAATCCTTGAAGATCAGAGTCAGATCGAATCTGGCCCGCTTGGAACCTTCGTCGGCGTTTACGACGGTCACGGTGGACCCGACGCAGCGCGTTACGTTTGCGATCACTTGTTCCGCAATTTTCAAGCGGCATCGAGTGAGTCACAGGGTGTTGTGACGGCTGAGACAATTCAAACCGCTTTTCGTTCGACTGAAGAGGGGTTTACTGCCATGGTTTCTGATTTATGGAATACTCGACCTCAAGTTGCAACTACCGGAACATGTTGTTTGGTTGGAGTGATTTTTCAGCAGACACTTTTTGTGGCTAATCTAGGAGATTCCCGCGTTGTGTTGGGAAAGAAAGTTGGGAATACCGGTGGGGTTGCGGCGATTCAGCTGTCGACGGAACATAATGCGAATCTTGAGGCCGTTAGACATGAACTTAAAGAATTACACCCTCATGATCCTCAGGTTGTTGTTCTCAAGCATGGAGTGTGGAGAGTAAAAGGCTATTATTCAGGTTT
TARGET=120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2945
PRIMER_LEFT_PENALTY=0.056850
PRIMER_RIGHT_PENALTY=0.237699
PRIMER_LEFT_SEQUENCE=CTTCACCACCTCTCGCTAACTT
PRIMER_RIGHT_SEQUENCE=CTTTGCCTTCTCTTCCAACAAT
PRIMER_LEFT=28,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=59.762
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=0.3938
PRIMER_LEFT_1_PENALTY=0.156135
PRIMER_RIGHT_1_PENALTY=0.237699
PRIMER_LEFT_1_SEQUENCE=GCCTCATTCTTAACCTACGCAC
PRIMER_RIGHT_1_SEQUENCE=CTTTGCCTTCTCTTCCAACAAT
PRIMER_LEFT_1=52,22
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=60.156
PRIMER_RIGHT_1_TM=59.762
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.4597
PRIMER_LEFT_2_PENALTY=0.056850
PRIMER_RIGHT_2_PENALTY=0.402858
PRIMER_LEFT_2_SEQUENCE=CTTCACCACCTCTCGCTAACTT
PRIMER_RIGHT_2_SEQUENCE=GGTGATATGAATCTCGCAACAG
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=159,22
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=59.597
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=0.5384
PRIMER_LEFT_3_PENALTY=0.156135
PRIMER_RIGHT_3_PENALTY=0.382228
PRIMER_LEFT_3_SEQUENCE=GCCTCATTCTTAACCTACGCAC
PRIMER_RIGHT_3_SEQUENCE=GCTTCCAACATGGAGAGAGAAG
PRIMER_LEFT_3=52,22
PRIMER_RIGHT_3=265,22
PRIMER_LEFT_3_TM=60.156
PRIMER_RIGHT_3_TM=60.382
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.5444
PRIMER_LEFT_4_PENALTY=0.056850
PRIMER_RIGHT_4_PENALTY=0.487583
PRIMER_LEFT_4_SEQUENCE=CTTCACCACCTCTCGCTAACTT
PRIMER_RIGHT_4_SEQUENCE=CCCACATCTCTCAGAAACCCTA
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=222,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=60.488
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_04574 Ps_contig_mira-and-tgicl-ass_franssen_33761
SEQUENCE=CTTTAAATTTACTACCTTGCTCACTTTCTCATTATCACCATAGTAAATAAAGCAAATATATATAGCTTATTTTCTAAATTTTGCATGATCTTATTTGACTTATTGCATCATGTTCAAATTAAATATTCACATCATTAACCTTCTCAAATGCATCATAATGCTGTTTATTTTCTAACATTCTTGCCACATAAGTTCCTAGTGCAACCACTGCCACTATTCCAAGCACAATTTGAATGGTTATTCTTTTCCTCTTTTGCACCCCCCATAATGAATGTTTCTCTATGGTTGCAACATCTTTGGCATGTTGAGTTGCTTCACTTTTTGGCAACACAATAGAAAGAATTCCTTGAGAAAACTTTGCTTGAATCCCACTCACATCACAATCTTTTGAAACCCTAATTTCTTTATGGAAACGACTCCATTTCTTACTAGATGCACTTTGAGGTTTTTCTCCATAAATTTTAGAACCCCTTTATTGTTGGTTTGAACCTTAAGTTGCTCTTTTTTGAAACCTTTCAAATCAACCTCAAGGATCTTTTGTCCTTCATTTGTTAGCCATTTACAATAAGGTTCAAAATCTTCATACAAACGTTTGCTACTATCTTCATTAGCCTTGGTTTCCATGGAAGATGATGGATGACTAGTGGCTTTTCTTTTCTTTTTTAACCTTGAGATTTTTCAATTTCCAAAAGTTATGAAAATATGTATTGAATTGAATCCCCGCT
TARGET=648,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04575 Ps_contig_mira-and-tgicl-ass_franssen_33766
SEQUENCE=AAATGAAAGATAGTGATAGAGTTATACATTTAAAATTAAATTTTCAAGTAACACAACTCTCAGGACACAAGAGAATATGTTCATTAAGGAATCTAACACTTGAACCATACAAATATATGTATTGATTTTTACATGACATGTATCTGCTATCACAAATTGAAAAATTCCTTTAATACTTGTTGGAGTTCATAGATCTGTTCCCAAGTATGAAAGCAATGCAATGCTATCCTTGTTTGTCCCCTAACTCATACAGCAGTTGGTGTTTCCTCTGAGGGCAGTTGCTTCTTACCTTTTGTGTTTCCTTTAATTTCCCATATATCCTTAATCTGAGTTTTTGGAGGTGGTGTTGCATCTACAATTGGAGCAACTAATCCTGGTTTTCCGTCATCAATAACCTCATCAACCAAACCGTATTCCTTCGCTTCCCAAGGATTCATAAAATTGTCACGGTCTGTGTCCAATTCAATCTGCTCTTCAGGCCTTCCCGTAATTCTTGATAGTATCTTGTTTATCTTAATCTTGTGGTATATCAATTCTCTCACACGGATGCACACTTCTGTACCTACTCCGCCAACAGACCCGAGCGGTTGATGAATCATAACTTTAGAATTTGGCATACAATATCTCTTCCCTTTTGTTCCTGCAGCAAGGAGAAATGCACCCATGGATGCTGCAAGTCCAAAGCAAACTGTTGACACGTCTGCGTTGCATAACTTCATGGCATCATAAATTCCCATTCCAGCCGTAACAGAACCACCAGGTGAATTTATAAACAACTTGATGTCTTTCGTAGGGTCATCAGCATCCAGAAACAAAAGCTGACTGATTACAAAATCTGCAGTCATATCATCCACCTGAGAACCCAAAAAGACAATTCTTTGACGGAGAAGCATGTTGGTAGTGTCAAGTTCTTCGAATTTGGGAAGCAATGGAGAAGCAGTTTTGGCAGATAAATCATGGGAAACGAGCCAGTTGGAGGATAAAGTGGGTTTGATTGCGTTAAAGATTGTTGTTCTGTGTTTGGTTGAAAGATTGAGGGTGGGAATTTGAGAGTGGAAAATGTTATTGTTATGCTTCAAGAGCGTCTTGTTCGATTTGAAGCTTAGTGGCATACATGTGGCTAAACTCACTTCCATTTTCTTCTTATTTTCTTCTTCTTCTTTGTTTTGGTAACTATCCCCGCGAATGTCT
TARGET=1149,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6453
PRIMER_LEFT_PENALTY=0.101680
PRIMER_RIGHT_PENALTY=1.543643
PRIMER_LEFT_SEQUENCE=GAAGCAATGGAGAAGCAGTTTT
PRIMER_RIGHT_SEQUENCE=AGACATTCGCGGGGATAGTTAC
PRIMER_LEFT=922,22
PRIMER_RIGHT=1190,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=61.544
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=269
PRIMER_PAIR_PENALTY_1=1.6740
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=1.543643
PRIMER_LEFT_1_SEQUENCE=GAGCCAGTTGGAGGATAAAGTG
PRIMER_RIGHT_1_SEQUENCE=AGACATTCGCGGGGATAGTTAC
PRIMER_LEFT_1=965,22
PRIMER_RIGHT_1=1190,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=61.544
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=1.7295
PRIMER_LEFT_2_PENALTY=0.185822
PRIMER_RIGHT_2_PENALTY=1.543643
PRIMER_LEFT_2_SEQUENCE=GGGAATTTGAGAGTGGAAAATG
PRIMER_RIGHT_2_SEQUENCE=AGACATTCGCGGGGATAGTTAC
PRIMER_LEFT_2=1040,22
PRIMER_RIGHT_2=1190,22
PRIMER_LEFT_2_TM=59.814
PRIMER_RIGHT_2_TM=61.544
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=151
PRIMER_PAIR_PENALTY_3=1.7876
PRIMER_LEFT_3_PENALTY=0.243965
PRIMER_RIGHT_3_PENALTY=1.543643
PRIMER_LEFT_3_SEQUENCE=GATAAAGTGGGTTTGATTGCGT
PRIMER_RIGHT_3_SEQUENCE=AGACATTCGCGGGGATAGTTAC
PRIMER_LEFT_3=978,22
PRIMER_RIGHT_3=1190,22
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=61.544
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=1.8026
PRIMER_LEFT_4_PENALTY=0.258908
PRIMER_RIGHT_4_PENALTY=1.543643
PRIMER_LEFT_4_SEQUENCE=GTTGGAGGATAAAGTGGGTTTG
PRIMER_RIGHT_4_SEQUENCE=AGACATTCGCGGGGATAGTTAC
PRIMER_LEFT_4=971,22
PRIMER_RIGHT_4=1190,22
PRIMER_LEFT_4_TM=59.741
PRIMER_RIGHT_4_TM=61.544
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_04576 Ps_contig_mira-and-tgicl-ass_franssen_33771
SEQUENCE=ATTTCARTCNNNNCTCTCCAGAAAGGGGCAAAACCTAAAGACCGTTTGCACCCACCAATTGTCATGGATGATGATGTGCTGAATATGCCCAATTGGGGTTACTATGAACCCTTCAAGGGTGGGAATCTCGGTCTGAATCTGATGCCTGATATTAATGATCGGGAAACCAAACCGTTTTTTCCTGGTCGTGATCCAGCTATGTTAGTCGGTGCGAATGGAAGTTTTCACCCTCGTGACAGTGCTGTTGTTGAGCCTCCTGCTCAGTTGAACTATGTCAGGGATAATTGGATGAATCCGAGACATAGGTTTTATAATATGCAGCTTTCGAATCCAAGTTATGCTGCTGTTCTTCCTGAAACATCGCAGGGACTCTCCTTGCCGTTTATCCAACCACTGGTGGATGCATCAAGAAACGAGAATGGGGATGGGATTGAAGAATTAGCTGTTAAGAAGGAAGGTGGCAAGTCGAAGAAAAGGAAGTCGAAGGGTGCTCTTGCCGTTCCAAAAGCTAAAAAAGCCAAGAAGGCAAAGGAGAACGGCAATAATTCAGCTCAGGGTGTGAAGCCACCAAAGAAGACTGTGGCGCTTGAAATAAATGGAATTGAAATGGACATTTCTGGTCTACCTGTTCCTGTTTGTTCATGCACCGGGAGTCCTCAGCAGAGTTATCGGTGGGGCTGTGGAGGTTGGCAATCAGCTTGCTGTACCACAAATGTGTCAATCTATCCTTTGCCGATGAGCGTCAAAAGGCGTGGCGCAAGGATAGCTGGGCGGAAAATGAGCCAAGGAGCTTTCAAAAAGGTTCTAGAAAAGCTTGCAGCTGAAGGTTATAATTTTGCTAACCCTATTGATCTGAAGACTCATTGGGCGAGACATGGCACCAACAAGTTTGTCACTATCAGATAGATGCTGCTGCTGCTGGGTTTGAAAGTCAGTCTGTTTTGCCTGTGGATGCACCTGTGTGATTTTTCATACAGATGTTGAATTCTGGTCATGGTCATCCATAAATTTTCTGTTGTGGTGCGTCTATCTAAGTTTTGGATACTCAGGTTTTTTTCCATCTTCAGGTTATTTGACAATGATGACAGTCCATGTTTTATGGAGTTAAACATTTATGTAGATGATTTATCATTAGATAGACTATTATCGGATAATACTCATTCACATTTAATTTAAAATCATCGAAATTTTGAATAGACGTGATCGTTGTTTCAAATATGTTTCACCCTCTCTTAATGGTTTATCAATTATGTGCTAGCTTTGATTGTTTGATT
TARGET=908,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04577 Ps_contig_mira-and-tgicl-ass_franssen_33775
SEQUENCE=AGTTATATTATTGGACATCATCGCCATAGAACCATACAACTGGCAATGTAGGAGAAGAATGGTACTAGGTTGCCTCCTCAACCAATGGAGRCTGTTAGATCTTTCCCATCTATATTTTGTATGTATGTATGTATGTCTAGATCTTCGCAATCTAGTTATCTTTAACTTTTGGTTTTCAGGTTCCAACCCTTTTGTATATATTAAGATAATATATGCAGTTTACTTAGTTTAACCATACCATTTTGCTCGTTGTCTGATTAGTTACCATAAGCTGGTCATTGTGTCTTTGGATAAAACATAAGATGTTCAGTGTTAACTCAATCATTTTCTTTTCTCTCAATTCCAAT
TARGET=118,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04578 Ps_contig_mira-and-tgicl-ass_franssen_33776
SEQUENCE=TTAGGAGTCCTTCTGTGGATCAGAATGATGAAGACGATATGGTCGACAATGATACAGGATTTTTGAATGTTGGAGATGATGAGATAATTGATGACGATGATGACGATGCTGATGGTTTTGCACCGGGTGCTGAAGGAACACAGCTAGAAAATAGTGGATGGTCTTCTCGAACCAGGGCTGTTGCGAAGTATCTTCAGACCTTGTTTGATAAGGAGGATCTACATGGAAGGCAGAGCCTGCATCTTGACAAAATATTGGTGGGTAAAACACGGAAAGAAGCATCAAGGATGTTTTTTGAAACACTGGTTCTCAAGACAAGGGATTATATTGATGTAGAACAGACAAAACCCTTTGCCAATATTAACTTACAACCTCGAGGGAAGCTTATGAAGACAGATTTCTGAAACCTCACCTAGGAGTGGCTGATATAGAGGTAAAGATCCACGCGGTGATAGCTTGGATTGAATCTGCAGGGTTGGGATAATCTTTTCAACATCACTCCCCTATTTTAGTTATGGTTTTTCATTAAAATATATTTTTTTGGGGATAGAATTTAGCCGATTAATTTTACCTCTTGAATGGTTGGGGGTTGAAGTGTCTCATGTATCATTTCATCCTATTGATTATTCTAGGTTTTAATGTATATTATTGTAAAAATATATCACCATTTGTAGCTTACTGGTGCTAATATGTAATGTGTTTAAAAACACATCCCTCCATGCAAGAATTCAGTTTTATCTAGGCTTCTAGTTTCTTTTACTAGAAAGTGCGTTGTACCTTATATACCAGTTGGTGGATACGGATCATCATGTCTTACCCATGTATTTGTATAGTATAACTGTGTAGTTTGTTATTATACTCTGTATACTTTATAAAGGCAATTAACGTTRGTGTTGTTGTTGTA
TARGET=892,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04579 Ps_contig_mira-and-tgicl-ass_franssen_33782
SEQUENCE=GGTTATCTATCTATCTATGTTCCTAGTTCTATATATACACCGTGACTTTGTCGCAACGCAGTTGGAGATGCTCATCACATAGCAATCTATCACTTTTCTCTTCTCCTTTCATTCAACCCAAGATCGCAATTTCAATACTCCATTTCTTGCATTACGAAAAAGAGAGATTAAGCAATGTCAGATGGTAATAATATTGATGACGATGTGAAGAATCAAGTTCACCGTGATAATAACAATAATTCTGAGGATGATCTCAAAGCTTCTGAGACAGATGCTAATAATGAAAACTCAATGCCCTCTTCTCAACAAGAGGAAGACGCTGTAAGGAAAAAGTATGGAGGGAGATTGCCAAAGAAGCCTCCACTAATATCCAAGGATCATGAACGTGCTTATTTTGATTCTGCTGATTGGGCTTTGGGGAAGCAAGGAGCACAAAAGCCTAAAGGACCACTTGAAGCACTCCGTCCAAAACTGCAGCCAACTCAACAGCAGGCTCGATCAAGGCGCTCAGCTTATGCTCCAGGAGATGGTTCTGAAGTTGACGGTGATATTGCGTCATCAGAGGATTCAAGTGCTACCGAAGATGTTGCTACTCAAGATGTTGCCGGTGATAAGAGTGATGCTGCTCAAGACCAAAGTTGCCATTAGTAGCTATGGGTTTGAGCTATAAATTGCATTTTGACAGTGGTGGCTTTCTTGTCTGAGTTTACATGCAAATGATGATGCATATTAGAGTTGGTTGTGCTAATAACTAGTTTGAAGCGGTTGTAANNYYTAGAACGGTCGGTTTACGTAAACCG
TARGET=4,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04580 Ps_contig_mira-and-tgicl-ass_franssen_33787
SEQUENCE=TCAACAACTCTGTTTTATTACTGCAACACATAATAATGCGATGAGTAACATTCTAGTATGAATTACAAAATATATCTCTGCAAAGCAAATTTTTAGTTACAAATAAATTTAATTGATGCAAACAAATTCAAAGTAACACTCGAAATTACTAATGATACAATACAATACAATCCAATGCAGAACCCATTAATATATGTCAGCTCAACTAGGTTCTCTTCAATCCCTCCACCGTGAACGGAACGTCGTAAACCTTCTCCATATTAGGATAAAACATTCCAAAATTTCTCTCTGAAGTAGGTCCCACCTTTCCATTCTCGTTAAAAAGAGCGAACAAATAAACGGTTAGATTTGCATTGGGCCGCAATGGGGTCCCACCGTTAGTGAGAATCTTTTTAACAAGGTTACCGTTGTACGCAGCAGCGTTCTCTGGGGACGCACCCACCTCATCACTATCTCCTTTAGAAGGCCACCCTGTCTCGCTCACAACGATCCTAACGTCGTCGTATTTGAGAGCCGAAAGCGCTGCAAAAACGGCGTCGATTTGTGCGTCGAAGATGTTAAAATACTTTAACCCATTACCCGGATCCACGTTACCAGGGTTCTCACGAAACAAAGCGTAATCTAATGAAATGACGTCAGCGTTTGACTCGTAGGCGAAAAACGGATACACGTTGACCATTAAATACGATGACGTTTCGCGGATGAAATCCAGCATGGGTTTTATAACGGATTCGACTAGTTCGGGTTGGAATGAACCGGTTGAAGATGGGTATGAGGACCCGAGAGCGGTGAGTGCGATGGGGGAGGAGATTTTGATGGAGTTATGGAGATTGTTCTTGATAAGAGCTTTGTGAATGTTTTTCATGGCGGGGACGAGGTACTTGGTGGTGTTGTGTGGGTCGACGAAGACTTCGTTGCCAACTGCGATGGCTTCGATGACTGTGTGGGGTTGGTAAACGATGACGTTTCTGTTGAGCCATGAGACTGCGTAGGAGAGTTTCTTCGCTGCGAAGAAGAGTTGTTCGTTGGGGAGGTTGACGGTTACTTTGATTCCGGATCCGGATAATGCCCGGAGAACAGCTCGGTCGGTGTCGTATAGTTTGACCCGGTTGATTCCTTGTGATTTGAGAAGGTTAACTACTTTGAACGCAGATGGAAGGTTGTTGGCTATGCGACCATAGTTTACTCCAATGTAACTACCTTCTGCAAGTGCAAGTAGTGCTGCAAGTGAGAAGATGGAGAGAATGAGTAGACGATGCATGGTGAGATAGCAATGAGTGGTAGTGAAGTATGTCCCCNAC
TARGET=155,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04581 Ps_contig_mira-and-tgicl-ass_franssen_33799
SEQUENCE=AGAAACCAACGACGTCGTTTCCACCCATTCTTCCCTTGCTTTGAAAGTTGAAATCGTAAAATGTAAAAGTCAAGTCCCTCGTTTCANNTCCTTCATTTTCTTTCTCTCCCTTTTCAGTTTCTTGGAAGAGAAAAAAAAAATGGCCGTAGCTGAACCCAAGCCTCACTCCGAACCTAAGGTCTGGAATTTCTTCAAGCTTCCTTTTCGCCATTCCACCACCACCTCTTCCACCGGAACGACGTCGTCTTCTCCTAACCTTCATCACCCTCATCATCATCAACATCACCATAACCCTAACAGTAACGCGCACAATCTTCCTCCGCTTGAAGGTTCCACTTCTCAATCTTCCAATTCTGTTTCCTCCGTTGCCAGATCGCTTCTCCCAACACGACGTCGTCTCAAGCTTGATCCTTCCAACAAGCTTTACTTCCCTTATGAGCCAGGAAAACAGGTTAGGAGTGCTGTCAGGATTAAAAACACCAGTAAATCTAATGTAGCTTTCAAGTTTCAAACAACTGCACCGAAAAGTTGTTTCATGCGTCCACCTGGGGCTATCCTTGCACCTGGCGAGAGTATCATAGCAACGGTGTTCAAGTTTGTAGAGCAACCAGAAAATAATGAAAAGCCCGAAAAAAGTGGACTCAAATTTAAAATCATGAGTTTGAAGGTCAAAGGTTCAATTGATTACGTCCCTGAGCTGTTTGATGAGCAGAAGGACCAGGTAGCAGTTGAGCAAATTCTGCGTGTTGTTTTTCTAGATCCAGAGCGCCCTAGTCCTATTTTGGAAAAATTGAAGCGACAGCTGGCCGATGCTGATGCTGCCCTTGAAGCACGGAAGAAACCTGCAGAAGACGTGGGTCCTAAAATAATTGGAGAAGGGCTTGTCATAGACGAATGGAAAGAGAGGAGGGAAAGATACCTTGCTAAGCAGCAGGGCGATGTGGTTGTAGATTCTGTATAGACTGTTGGTCTGGATAAACTAATGTTTGTGTTATCTTGAGAGAAGATTATTAAAATCACAGCGTCTTTTTCCTGGAAAAGTTTCAGGTGAATGGCAAGTGTGTGCTATAGAGAAAGCTATGTGTGGGTGTGTTGATCATTGTCTATGTAGATAAATGTCTCATGTTTGTGAAATAATATATTTTGATAGATTTATGTCAAATGATCAGATAATGATTTGCTGTAGTCAATTTTATTTTGCCATGGTTCTACTTGTATGCAGATAGTCGACATTCTACC
TARGET=268,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1654
PRIMER_LEFT_PENALTY=0.164639
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=TCTGGAATTTCTTCAAGCTTCC
PRIMER_RIGHT_SEQUENCE=AACCTGTTTTCCTGGCTCATAA
PRIMER_LEFT=179,22
PRIMER_RIGHT=453,22
PRIMER_LEFT_TM=59.835
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.1911
PRIMER_LEFT_1_PENALTY=0.164639
PRIMER_RIGHT_1_PENALTY=0.026443
PRIMER_LEFT_1_SEQUENCE=TCTGGAATTTCTTCAAGCTTCC
PRIMER_RIGHT_1_SEQUENCE=CACTCCTAACCTGTTTTCCTGG
PRIMER_LEFT_1=179,22
PRIMER_RIGHT_1=460,22
PRIMER_LEFT_1_TM=59.835
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=0.2533
PRIMER_LEFT_2_PENALTY=0.164639
PRIMER_RIGHT_2_PENALTY=0.088662
PRIMER_LEFT_2_SEQUENCE=TCTGGAATTTCTTCAAGCTTCC
PRIMER_RIGHT_2_SEQUENCE=TCCTGGCTCATAAGGGAAGTAA
PRIMER_LEFT_2=179,22
PRIMER_RIGHT_2=444,22
PRIMER_LEFT_2_TM=59.835
PRIMER_RIGHT_2_TM=60.089
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.2533
PRIMER_LEFT_3_PENALTY=0.164639
PRIMER_RIGHT_3_PENALTY=0.088662
PRIMER_LEFT_3_SEQUENCE=TCTGGAATTTCTTCAAGCTTCC
PRIMER_RIGHT_3_SEQUENCE=TTCCTGGCTCATAAGGGAAGTA
PRIMER_LEFT_3=179,22
PRIMER_RIGHT_3=445,22
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=60.089
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.3010
PRIMER_LEFT_4_PENALTY=0.143968
PRIMER_RIGHT_4_PENALTY=0.157055
PRIMER_LEFT_4_SEQUENCE=AAAAGTCAAGTCCCTCGTTTCA
PRIMER_RIGHT_4_SEQUENCE=AAGATTGTGCGCGTTACTGTTA
PRIMER_LEFT_4=64,22
PRIMER_RIGHT_4=315,22
PRIMER_LEFT_4_TM=60.144
PRIMER_RIGHT_4_TM=59.843
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_04582 Ps_contig_mira-and-tgicl-ass_franssen_33808
SEQUENCE=GGAAATAAAAGACAACCACAAAGTACAAGATTTCTTTCTCCATGTTCAATCTATATATTCTGAAAGATTTCCTCTCAAGATTTTGCTTAAACTTCTTCATGAATCCCAAGCTTAAAACAGCTTTAACCGCAAAATGTTATGACACAAGGACAAATATATCTGATCCCTTCAAGCTATGAAAAGTAAATAACAGTTTCATGAACAAATAGTAGGACTTGACCTTAAAACACCAAATTATTATTGTCAAACCTTTCTTACAACTGTATCAACTAAAGAACTCCAAAAAAGGGATTCTTTATTGCAGAGTCTAATTCAGATCCAGCTGAATTCAGAACTGGAATCTCAACCTTGTCCCTAGGCAAAAACAAAACTTCACCGCCATCGATTTTCTCAAACCCGCAAATTTCAAGGAATTCAATGCCTCCTTTCAAAGCACCAACTCTTTCCTGAAAAGCTGCATTACTAAGGCGAATCTTCCTGAATTTTTCTTCATCAGGATTTCTGGCAACATTCCCCACATAAGTTAGAAGAGTTTGGAATGCTCTCTTCACTCTGGCATCATCATCCTTGTGGTTCTGCTTGAGAGAACGCAAGCACTCTCTCATTTGCTCAGTTTTTGTGGCAGGCCTAATTGGCAGGAAACTCTTTTTCTCCTCTACAACAGTTGAAGGTTTGGCTGTTGAAGGCTCCTCTGGGGGCAATCCAAGTTTTCGCCTTCTTTCTGCCTTGTCTTCTTCCAACTTTTGCCTGATTTTTTCTCTAGCCCTTCTCTCTTCCTCTTTCTCTGCTTTTCTCAAAGCTAACAACCGTTTTCTCTCGTTATCTTCTTCAATCCTCTTTGCTTCAAGCAACTCCTTGCCAATTCGAATTCTCTCCTTTTCTCTTTCTCTCTCTGTTCTCTTCTCTTCTTCTTCTTTCTTCTTGCGAGCCTTCTCCCTTAATTCCTTTAGTATGGCATTCGTTTCTTCTGGGGTAAGAGAGGGTTTAGTAGCCTTAGTTTTACTGACAGCAGATACCAAGGGCATCTCGTCTATGTCAGGAGTATTCTCATGGTTTTCTATCCAAGTTACAGCAGCCTCGAGGCTAGCATTACCAGAGAAATGAAGTGCCCTTGTTGCACGAGCTGTTGAAAAGCCCATTGATTCAAGTTCCTCAAGCAGAGTTTTATCAACTTCAGGAACAACCATTTCTTCATTTTGATCACTGGTTCCACCCTCTTGTGATTCTGAGCCCCCCTTAGGAACCTCTAAACTTATTGGTTTTGCAGCTTCGGCAGTCTTATCAACAAACCCAGTGTGTCCGGTTCTCTTGGTGTGTAAATCACTCTCGGTTTTGGATCGGCAGGGTTTGGAGCAAGTGGTGCAGACGAGATTTAGAACGGGTTCGGTGGATTCGGAGAAATTGGAGTGTGAAGTGAGCTCAGCGTGTTCTTGAGCTTCTTCGACCGATTTCAATAGAGCGCCGCAATCGCCGCACTGGAGCGAAACCCCTGCCATTTTCCGGTGGCTTTTTCGTCGGTGGATCCAATTGGGGAAATAGAAACTCCCCGCGTACTCTGTCGTTGAACTACCACTTCGT
TARGET=904,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0067
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.004430
PRIMER_LEFT_SEQUENCE=TCTTTCTGCCTTGTCTTCTTCC
PRIMER_RIGHT_SEQUENCE=AAACCCTCTCTTACCCCAGAAG
PRIMER_LEFT=716,22
PRIMER_RIGHT=985,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=270
PRIMER_PAIR_PENALTY_1=0.0565
PRIMER_LEFT_1_PENALTY=0.052069
PRIMER_RIGHT_1_PENALTY=0.004430
PRIMER_LEFT_1_SEQUENCE=AGCTAACAACCGTTTTCTCTCG
PRIMER_RIGHT_1_SEQUENCE=AAACCCTCTCTTACCCCAGAAG
PRIMER_LEFT_1=797,22
PRIMER_RIGHT_1=985,22
PRIMER_LEFT_1_TM=59.948
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=0.0825
PRIMER_LEFT_2_PENALTY=0.078029
PRIMER_RIGHT_2_PENALTY=0.004430
PRIMER_LEFT_2_SEQUENCE=CTGCCTTGTCTTCTTCCAACTT
PRIMER_RIGHT_2_SEQUENCE=AAACCCTCTCTTACCCCAGAAG
PRIMER_LEFT_2=721,22
PRIMER_RIGHT_2=985,22
PRIMER_LEFT_2_TM=59.922
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.0837
PRIMER_LEFT_3_PENALTY=0.078029
PRIMER_RIGHT_3_PENALTY=0.005674
PRIMER_LEFT_3_SEQUENCE=CTGCCTTGTCTTCTTCCAACTT
PRIMER_RIGHT_3_SEQUENCE=CTTACCCCAGAAGAAACGAATG
PRIMER_LEFT_3=721,22
PRIMER_RIGHT_3=976,22
PRIMER_LEFT_3_TM=59.922
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.1791
PRIMER_LEFT_4_PENALTY=0.174657
PRIMER_RIGHT_4_PENALTY=0.004430
PRIMER_LEFT_4_SEQUENCE=GCTAACAACCGTTTTCTCTCGT
PRIMER_RIGHT_4_SEQUENCE=AAACCCTCTCTTACCCCAGAAG
PRIMER_LEFT_4=798,22
PRIMER_RIGHT_4=985,22
PRIMER_LEFT_4_TM=59.825
PRIMER_RIGHT_4_TM=59.996
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_04583 Ps_contig_mira-and-tgicl-ass_franssen_33812
SEQUENCE=AACGCAGAGTACGCGGGGAATCTAATCTGAAAAAGTTGCTAGCTATGCCAATTTCCCAATTATGTCCAAACACCCATCCAATTCTACAAATCAAAGCCTGCAACCGTAACAACAACAACGACACTGGATCCAAGTCAGTTACAGGAAAACCCAGGGTCAGTCCCCAGCTGAACCGGTGGTCCAGGGCTCGTGCAATACGTTCCGGTCGAAAATTGGACCGCTCTAGCCCACGAACCCAAACCCTAGAACCCAACCATTCTCCCCCTACAGTTCCCTTCGCTGTCGATCCAGATGACACCGTCACTGAAGACGGCTCAGAGTTTAGGGCCAAATCCATTTATATTGTCTCTGATGGAACCGGTTGGACCGCTGAACACTCCGTTAATGCCGCTTTGGGTCAGTTCGATTACTGCTTGGTTGATCATGGCTGCCCCGTTAGTACCCATTTGTTTTCTGGGATTGATGAGGTGGAAAGGTTATTGGAGATAATAAAGCAAGCAGTGAAGGAAAATGCATTGCTGGTGTATACATTAGCTGACTCTTCATTATCTTCAGCGGCTGAGAAGGCCTGCAAATTATGGGGTGTGCCTTCTACTAATGTATTGGGACCCATTACCGAGGCCGTTGCTTCTCATTTGGGTGTGTCTCCTTCTGGCCTTCCTCGTGGAGCTTCCGGCGTTCCTCTTTCCGATGATTACTTTCGTAGGATTGAAGCTATTGAGTTCACTATCAAGCAAGATGACGGGGCATCTCCGCAGAATCTTGCCAAGGCGGATATTGTGCTTACTGGGGTTTCGCGCACTGGGAAGACGCCTTTGTCTATTTATCTTGCTCAGAAAGGGTATAAGGTCGCTAATGTACCGATTGTGATGGGCGTGGATATGCCTAAGACTCTCTCTCAGGTTGATCCAAAGTAAAGTTTTTGGATTGACTA
TARGET=108,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2210
PRIMER_LEFT_PENALTY=0.121675
PRIMER_RIGHT_PENALTY=0.099308
PRIMER_LEFT_SEQUENCE=GTTGCTAGCTATGCCAATTTCC
PRIMER_RIGHT_SEQUENCE=CCTGGGTTTTCCTGTAACTGAC
PRIMER_LEFT=34,22
PRIMER_RIGHT=154,22
PRIMER_LEFT_TM=60.122
PRIMER_RIGHT_TM=59.901
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.2304
PRIMER_LEFT_1_PENALTY=0.121675
PRIMER_RIGHT_1_PENALTY=0.108707
PRIMER_LEFT_1_SEQUENCE=GTTGCTAGCTATGCCAATTTCC
PRIMER_RIGHT_1_SEQUENCE=AGAATGGTTGGGTTCTAGGGTT
PRIMER_LEFT_1=34,22
PRIMER_RIGHT_1=259,22
PRIMER_LEFT_1_TM=60.122
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.2887
PRIMER_LEFT_2_PENALTY=0.179943
PRIMER_RIGHT_2_PENALTY=0.108707
PRIMER_LEFT_2_SEQUENCE=AATTATGTCCAAACACCCATCC
PRIMER_RIGHT_2_SEQUENCE=AGAATGGTTGGGTTCTAGGGTT
PRIMER_LEFT_2=57,22
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=59.820
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.3790
PRIMER_LEFT_3_PENALTY=0.179943
PRIMER_RIGHT_3_PENALTY=0.199067
PRIMER_LEFT_3_SEQUENCE=AATTATGTCCAAACACCCATCC
PRIMER_RIGHT_3_SEQUENCE=AATGGATTTGGCCCTAAACTCT
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=337,22
PRIMER_LEFT_3_TM=59.820
PRIMER_RIGHT_3_TM=60.199
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.4748
PRIMER_LEFT_4_PENALTY=0.275749
PRIMER_RIGHT_4_PENALTY=0.199067
PRIMER_LEFT_4_SEQUENCE=CAAACACCCATCCAATTCTACA
PRIMER_RIGHT_4_SEQUENCE=AATGGATTTGGCCCTAAACTCT
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=337,22
PRIMER_LEFT_4_TM=59.724
PRIMER_RIGHT_4_TM=60.199
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_04584 Ps_contig_mira-and-tgicl-ass_franssen_33814
SEQUENCE=CAAATCAATTTAGCCATTTTAAGCCAAACACAATTCTTACAATCAAAATGATCTTTATTGCCACAAGCTGGATGCTGAATAAGACATTGTCCTGGGTGTTTTTTGATGATTGCATACAAAACATACCCCTTCAAACTATTATTCATTGAAAAGAACATAGCCATCATAACATTAAGTTTCAGGAGGAAAATCACAATCTTGGCGGATAAGTTGTTGCTAACGACTGAGATGGCCGAGCTAATCAACAGCAAGTTATTACAATCCTACCTACATCTGGATTTCTGGAAAAGTAAATATCCTCAAATTATCCTACAACACATGACATTGACATGATCATAGAATAGAATAGAGATCATTTTCTAAGCTTCTCCTTCATCTTCTCTACAGCAGCCCTAAGAGTTCCTTCATCCTTGCAGAAAGTAAACCGGACTAGATTTTTTCCCTCTTCTGGATTCAAGTAAAACACACTCGTAGGAATTGCCACCACTCCCACCTCCTTAACAAGATATTCGCAAAATTCAACATCGTTTTCATGTCCAAAAGGGGTGTGATCTACAACCACAAAGTATGTTCCATTGGACGGGAATATCTTGAAGCCAACATCCTTCAACCCTTCAACCAAAATAGCTCTCTTTGCCATATAATCCCTCTTCAATTCAGTGTAATAAGAATCTGGTGCTCTTAGAGCTACTGCAGCAGCCCGCTGCATAGGATTTGAGGTTGCAAAAGTGAGGTAAGCATGTGCCTGTCGCACTCCCCATGTTAAGTGAGGAGGTGCTATGGCCCAGCCGATTTTCCATCCGGTCAAGGAGAATGTCTTCCCTAACGAGTTCATTGTCACCGTCCGCTCGAACATTCCAGGCAAAGAAGCAATAGAAATGTGTTCCAAATCAAATGCCAACTTGTCATAAACTTCATCACTGAACACCAAAACATCATTCTCGATGCAAAGAGATGCAATGGTATTGAGCTCCTCTCGAGTGAACATCTTTCCGGTAGGATTGTGAGGGGTATTTAAAAGAATGGCACGAGTATTCTTTGAGATGGTGGACTTCAACTCTTCAATCGGGAGAGCAAAATCTGGAGGGCGCAAAGTAATGCCTTTTACTTTTGCACCAGCCATGGATAAAGTGGCTTCATAAGAATCATAAAAAGGAGCAAACACAATAACCTCATCACCCGAATTTATCAATCCTAAAATAGTTGCAGCAATTGCTTCTGTGCACCCAGACGTAACAGTAATTTCCTTTTCAGGGTCCACCACTAGTCCAGTATCTTTCTTGAACCTCTCAGCAATGGCAATATTGAGGTCTGGAACTCCATAACCCCTTGCATACTGATTATTCCCATCTCTGATTGCCTGAATTGCTGCTTCCTTTACGAAATCCGGACCATCGAAATTGGGAAAACCTTGACCAAGGTTTATGGCTCCATGTTTGATGGCAAGCATACTCATTTGGGTGAAAATTGTAGTTTTGAACTTCTCCAACCGCTTTGCAACCTGCAAAGGCTGAGTTTCTGTGGAAACGGTGGACATGGCGGCGGAAAGGAGAGGTTTGGAGAAATAGGGGGTGTTGAAATGATTAGAAAAGCGTAACAATTTGGAGGAGGGTATGAATCTGAAGAGAGAGTGCGTGCAGCAAGGGATATTATAGAGCTGCATAATATAGTACTAATAAATGAAATTTGATTCTATCTGATTTATGCTCTTGACAGTGCCGTTTATGGTAAG
TARGET=336,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2329
PRIMER_LEFT_PENALTY=0.137550
PRIMER_RIGHT_PENALTY=0.095396
PRIMER_LEFT_SEQUENCE=ATCACAATCTTGGCGGATAAGT
PRIMER_RIGHT_SEQUENCE=AGGATGAAGGAACTCTTAGGGC
PRIMER_LEFT=189,22
PRIMER_RIGHT=410,22
PRIMER_LEFT_TM=59.862
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.2329
PRIMER_LEFT_1_PENALTY=0.137550
PRIMER_RIGHT_1_PENALTY=0.095396
PRIMER_LEFT_1_SEQUENCE=ATCACAATCTTGGCGGATAAGT
PRIMER_RIGHT_1_SEQUENCE=GGATGAAGGAACTCTTAGGGCT
PRIMER_LEFT_1=189,22
PRIMER_RIGHT_1=409,22
PRIMER_LEFT_1_TM=59.862
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.2520
PRIMER_LEFT_2_PENALTY=0.137550
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=ATCACAATCTTGGCGGATAAGT
PRIMER_RIGHT_2_SEQUENCE=CTGCAAGGATGAAGGAACTCTT
PRIMER_LEFT_2=189,22
PRIMER_RIGHT_2=415,22
PRIMER_LEFT_2_TM=59.862
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=0.3791
PRIMER_LEFT_3_PENALTY=0.137550
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=ATCACAATCTTGGCGGATAAGT
PRIMER_RIGHT_3_SEQUENCE=TTTCTGCAAGGATGAAGGAACT
PRIMER_LEFT_3=189,22
PRIMER_RIGHT_3=418,22
PRIMER_LEFT_3_TM=59.862
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=0.3791
PRIMER_LEFT_4_PENALTY=0.137550
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=ATCACAATCTTGGCGGATAAGT
PRIMER_RIGHT_4_SEQUENCE=CTTTCTGCAAGGATGAAGGAAC
PRIMER_LEFT_4=189,22
PRIMER_RIGHT_4=419,22
PRIMER_LEFT_4_TM=59.862
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_04585 Ps_contig_mira-and-tgicl-ass_franssen_33820
SEQUENCE=CCCGTACCCTCGCACGGGAGATTTTAGTTTTGGGTAAGTTCAGGCATGGCAGATGCTTTGACGGTATCAGCTGATCAAAGAGACAGATTAATGTTTTCAACTGAGAGGCTGAACAAGTCTGGTGACAGAATTAAGGACAGTAGAAGAACAATGTTGGAAACAGAGGAGCTTGGAGTGTCGATCCTTTCAGACTTGCATTCTCAGCGACAATCTTTACTGCACGCACATGACACACTTCATGGAGTGGATGATAACATAGGCAAGAGCAAGAAAATTTTGTCGAACATGTCTAAGAGGATGAACAAGAACAAGTGTATTATTAGCACCATAGTTGTGGTCCTGGTTCTTATTATCGGCGCAATTCTGTACTTTAAACTTTCAAAATAGACAATAGAAGATATTTCTCTTATATCGCCACGACGCAAAGCTTTATAGAATTTCGCATGTTAATGTTGTGCAATGCTGCAAGCAAATTTGAAAATATTTGCTTCACAAGTGTGTGTGTGTCTTGTGTTGGCTTTGTCATGTAAGATTTGTAAGTATTTTGTGATCAGTTATTGTGTGAATTTTATATGATACTATTAAAATATACTGTTATGTTGTAAAATCTATCTAATCTATTGGATTAAATGTTAGTAAACCAAAAAAATT
TARGET=496,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2659
PRIMER_LEFT_PENALTY=0.016182
PRIMER_RIGHT_PENALTY=1.249678
PRIMER_LEFT_SEQUENCE=ATTATCGGCGCAATTCTGTACT
PRIMER_RIGHT_SEQUENCE=CTTACATGACAAAGCCAACACA
PRIMER_LEFT=348,22
PRIMER_RIGHT=530,22
PRIMER_LEFT_TM=60.016
PRIMER_RIGHT_TM=58.750
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.1000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=1.2836
PRIMER_LEFT_1_PENALTY=0.033891
PRIMER_RIGHT_1_PENALTY=1.249678
PRIMER_LEFT_1_SEQUENCE=GTTCTTATTATCGGCGCAATTC
PRIMER_RIGHT_1_SEQUENCE=CTTACATGACAAAGCCAACACA
PRIMER_LEFT_1=342,22
PRIMER_RIGHT_1=530,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=58.750
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=1.4028
PRIMER_LEFT_2_PENALTY=0.153161
PRIMER_RIGHT_2_PENALTY=1.249678
PRIMER_LEFT_2_SEQUENCE=ACCATAGTTGTGGTCCTGGTTC
PRIMER_RIGHT_2_SEQUENCE=CTTACATGACAAAGCCAACACA
PRIMER_LEFT_2=324,22
PRIMER_RIGHT_2=530,22
PRIMER_LEFT_2_TM=60.153
PRIMER_RIGHT_2_TM=58.750
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=1.4465
PRIMER_LEFT_3_PENALTY=0.196811
PRIMER_RIGHT_3_PENALTY=1.249678
PRIMER_LEFT_3_SEQUENCE=TGGTCCTGGTTCTTATTATCGG
PRIMER_RIGHT_3_SEQUENCE=CTTACATGACAAAGCCAACACA
PRIMER_LEFT_3=334,22
PRIMER_RIGHT_3=530,22
PRIMER_LEFT_3_TM=60.197
PRIMER_RIGHT_3_TM=58.750
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.8000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=1.5307
PRIMER_LEFT_4_PENALTY=0.281044
PRIMER_RIGHT_4_PENALTY=1.249678
PRIMER_LEFT_4_SEQUENCE=CCATAGTTGTGGTCCTGGTTCT
PRIMER_RIGHT_4_SEQUENCE=CTTACATGACAAAGCCAACACA
PRIMER_LEFT_4=325,22
PRIMER_RIGHT_4=530,22
PRIMER_LEFT_4_TM=60.281
PRIMER_RIGHT_4_TM=58.750
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=206
=
PRIMER_SEQUENCE_ID=SSR_04586 Ps_contig_mira-and-tgicl-ass_franssen_33825
SEQUENCE=GGGTAGGGTAAAACGGGGAACGGTTTAGNTCATGAAAAGACCCCAAACTACCTCGAATCYAGCAAAGGGAAGTTTGTCTGGGTCCATTAACATATGCTGCTGCTGCTGCTGAGGCGGAAACTGTTGATAGGGACCAAATGGATTTGCTTGCTGTTGCATTGCTGCCATTTGGACAGCGGTTGGGGGGGCGACACTGCTTGATACAGCAAACGGATCATGAACTTCAAATGGATTTGGAGCTGGTGCTCCATAAACTGGTTGCTGCGCAGCTCTATACGCACCTTCATCATATAAGCTGTTAAGAGTGAGAGAGTCCAGTCCACCAGCCAATTGTCTCTCGTTGACTGAAGAAATATCTGTACTTGGAGTGCTAACCAGGGCAAGCTCCCATCCAGTAGGGTCGAAATTTTTTGTTTGAGCAGCACTAGAGTTAAAAGCTGATGCAGTTCCATTTTCAGTGGAGACTATGGCCAGCGCAAGGGCGTTTCTTTCCTCTATGGATGATGCATTAGGTTCCGGGTCATTCAATCCCAGTAAGTCTCCAGTGTCAAAATTGTTGTGAGATGGTGGTGGGGGAGGAGGAGCAGCCTCAGAATTGGGGACAACAACATTATTATCAATAGGTTCAGGTTCTGATGGCATCTGTTCTTCAGACTCTTTGGTGTATTCAATTGCAAGAACTTCGTCTGGTCTGTAAGTCAACTGAAGCATGGGCTCATTTGGAACTGAAACCACTCGGGGTGCCTCCCTAATGTACTCTTCCATAGTTGTAAGAAAAGATTGCGGGGGCTCTCTTAAGACAGGAAACTGAAAATTTCTAGCGAGTTCCAATCCTTTGCAAACCTCATAGAAATCAGAAAGGCTTGCTGCCTGTTGTCCCGCACGTTTATAGGCTTCAAGGGCTTTAATAGCTTCATGTCTTGGCATCTCAAAAAACTTGTCAACAAGATTGATAATGCCATCATTAATAGCACAATAAATCTTAAAGCTCTCTTTCAGTACCAGAGCCAGAGCATATTGTATCACATAATTGCTAACAGCTGCTCCTTCTGGCCGACAGCCAACAAGTCGATATAGCAGCTGTTGCAAAGCAGGCAACTGCTCCAATAGCTCCTCACTATCTAAATCCCTGGTCTTGCTGTGTCCCTTCTCCTGTCCTTGGGAAGCTTTGGGTAGACGCTCAGCTTCAATATCATACTTAAGAATTCGAAAGCATTCAAGTCTTTCTTCCAAAAATAATGCATAGGTACCGTACCCAGGACAAGAAACCAACTCCCGAGGCTAAGTTAGGAC
TARGET=95,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04587 Ps_contig_mira-and-tgicl-ass_franssen_33835
SEQUENCE=ACTTTTTATCCACTAAATTGAAGAGATAAATATTATGGTTTATTATTCTATAATATCCGATAGAAAACAACTTTATACTAGCACCAGACACACCATTGGAGAACAGTATCTATTTCAATATTGTTGGCAGCAATGTCCAATATTTTATTTGCAACTAGTCGGGGCAAACACCATCAAGGTTTTCATCAATTAAATTAAGTAAATAGAAATTGACCGCCACATGACCACATCATCACATGCAATATGAGAACAATTGGCCCTAAAAAAATGTTGTTTCTCAGAACAAGTTTACATAAGCAATGGCATCCGTGCAGAATACAAATATTCCAGGGGCAACGAATGCAATCTATGTCTTCAAACAGCAACTTGTCCAAATAGTTGTTTTCGAATCTCTGGAAGCTTTTTGCGGAACACTACATCCAGCCGTGCATCAAGAGTATTTTCACACACAATCTTTCCATCGCGAGAAGCCAACACCACCCCACCAGAGCAGTAGGGATCATGAGGATGGTGATGCTTGGGTGCAGGTGGAAGATATACATCATGGTCAACAACGATCTCTGGTGGAATAACACCTGCTTTTTCAGCATACTCCTCTGCAGCTGCTTCCAGCACATGTTCTACCATGTGCACGTCACGTTTCCGACATCTCAACAAGACAGAAGGTTCTTTCAGTCTTAGCAAACTCTGAACAATGAGATCTTTAAGAAGGTTTCTGTACTCATGATGACCCACATGCAAAATATCCTCCACAAGGTGATGATGGCTCACATTCAAAAGTTCCTTAGCTGCAACTTCTTTCATGGAATTAACAATGTCATCTTGAGCTTGAAGAACTTTGATCCGAGAAGCATTCAGCTGCATCGAGTACTCAATTTTCTTTCGAATTTCAACTTGGCGCTCTTTGCGTTCGTATTCTTGTCTGATCTTCTTCTTCTCGGCTTCAACCAACTGAAGCTTCTCGATATTGAATTCCTCTTCGGCAGAAGCTGAGATCTCGGTGGCTTTTTCTTCAGCTTCTTGTTGGATAAACCTCACCATCTGGTGGATTTGACGGGATACATCATCCTCGTTCATTTTGGGAGAAGATTCACCGGGAAACAGAAACTACTTCGTCAATGGGCGATTGTACCAGTCACCAACCAGAGTAATAGCCCCCTACAAGAGCCAGCTAAGCTCTTTTAT
TARGET=927,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2712
PRIMER_LEFT_PENALTY=0.225403
PRIMER_RIGHT_PENALTY=0.045749
PRIMER_LEFT_SEQUENCE=TGCGTTCGTATTCTTGTCTGAT
PRIMER_RIGHT_SEQUENCE=ATGAACGAGGATGATGTATCCC
PRIMER_LEFT=905,22
PRIMER_RIGHT=1076,22
PRIMER_LEFT_TM=59.775
PRIMER_RIGHT_TM=60.046
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.2803
PRIMER_LEFT_1_PENALTY=0.225403
PRIMER_RIGHT_1_PENALTY=0.054900
PRIMER_LEFT_1_SEQUENCE=TGCGTTCGTATTCTTGTCTGAT
PRIMER_RIGHT_1_SEQUENCE=GAATCTTCTCCCAAAATGAACG
PRIMER_LEFT_1=905,22
PRIMER_RIGHT_1=1091,22
PRIMER_LEFT_1_TM=59.775
PRIMER_RIGHT_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.3149
PRIMER_LEFT_2_PENALTY=0.269153
PRIMER_RIGHT_2_PENALTY=0.045749
PRIMER_LEFT_2_SEQUENCE=GAACTTTGATCCGAGAAGCATT
PRIMER_RIGHT_2_SEQUENCE=ATGAACGAGGATGATGTATCCC
PRIMER_LEFT_2=833,22
PRIMER_RIGHT_2=1076,22
PRIMER_LEFT_2_TM=59.731
PRIMER_RIGHT_2_TM=60.046
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.3434
PRIMER_LEFT_3_PENALTY=0.297699
PRIMER_RIGHT_3_PENALTY=0.045749
PRIMER_LEFT_3_SEQUENCE=TTTGCGTTCGTATTCTTGTCTG
PRIMER_RIGHT_3_SEQUENCE=ATGAACGAGGATGATGTATCCC
PRIMER_LEFT_3=903,22
PRIMER_RIGHT_3=1076,22
PRIMER_LEFT_3_TM=60.298
PRIMER_RIGHT_3_TM=60.046
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.3526
PRIMER_LEFT_4_PENALTY=0.297699
PRIMER_RIGHT_4_PENALTY=0.054900
PRIMER_LEFT_4_SEQUENCE=TTTGCGTTCGTATTCTTGTCTG
PRIMER_RIGHT_4_SEQUENCE=GAATCTTCTCCCAAAATGAACG
PRIMER_LEFT_4=903,22
PRIMER_RIGHT_4=1091,22
PRIMER_LEFT_4_TM=60.298
PRIMER_RIGHT_4_TM=59.945
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_04588 Ps_contig_mira-and-tgicl-ass_franssen_33836
SEQUENCE=AATCATGAAAGAGAGATGAGAAAGACAGAAAAAGAGTGAGAGAGAGAAGCAGTACTTTTCAGAATAAGCGGTTCACTGTGTCTCTCTGACTTCGATGATGCATGCAAGAGGAACAAGATGATGATGCAAAAATTCTTCTGATTATCAAACTAAGAATGTGATGATGCTGGAAGGAAGTGCTGATTTCGTCGTTTGAAGAGGAATAATGAGGTACTAAGGATAATTCTTGGAAGTATTTTGATATCATGGCCGATTCTTCTTCCAATTTCTCTTTACCTTCTGAAACAATAAGCCCGAGAAAAATATGTTCCTCAATTTTTCACGCTTCTGGTTCTAGGATCGGAGTTGGTGTGAAAAACTTACCAGATTTTGAATCCGTCTGGAGTCCTACATCTCCTCTTGATTGCAGACTCTTCTCGAATCTTAGCAATGTGTTCGGTGTCAAGTCTTCTAGGAAAACTGAGCATAAGAAGCCGTTCGATGGAAGTAAAGTAGGCCTTGGTATCATAACTTCTCTTGTTAACGAAACCAAATCTAGTAACGAGATCCTTGGTGAATTTCAGAGGAAAAATATAATTTTTGGATCAGAGGTGAAGAACGGCATACTTCAATTCTCGAACAAAAACCTTGAATCTCTTGCATCTTGTTTGAAAACCAATTCCTTGCCCAAAAACTATGTAATTTCACTTCCTTCTGAAACCAAAAGCCGCTTATCGGAAGTTGAAAGATTTGATGATGATGTCAATTGGGAATCTTTGCCTGATTCCTCTAGACCTTCTTCGTTAATCAACTTAACTCAAAGCTCTAATTCGGGAACCGATGATTTATTTGTAGAAGTTACTTCTGCAACATCGAGTTTTGCCCCGGTTATGAACAGAAATTCACCAGTAGATGATTCTTTAAAAATCAAATCATGTCCACTTCCGATATACGTAGGTTCACTCTCTGCAAAAGAGATAGAGCTTTCAGAGGATTATACTTGTATAATCTCTCACGGTCCAAATCCTAAGCGAACACATATATTCGGTGACTGCATTTTGGAATGCGACAACAACGACTTTACCGAGTTCAGCAAGAAAGAAGAACCAGCTTTCAAATCTTCTCAAGAATCTGCGCCTCATCGTTTCGACAGTGTTACGAGCTTTTGTTACTCGTGTAGTAAGAAATTAGACGAGGAGGAAGACATTTACGCATATAGCGAAGAGAAAGCGTTTTGCAGTTTTAAGTGTCGATCGGAGGAGATTTTCGCAGAAGACGAAATGGAGAAAACTTGCACTAACTCAGAAGAGCTCTCTCCTAACTCGAGTTACCATGACGATATCTTCCTCATGGCTCTGCAAGTTTCCAAATAACATGCTTTATTACGAAACTGTTGTGAAGGTAAAAGCGTAGACAATCGGCTATGTGGATTTGTTTTTGCTTTCGGCTTTTGGTTATTGATGTGCAAAAGTATGCTATGTGTTAGCTGTCAAGAACCGAATTTCATGGTTTTGTAGCTATAGGAAAGGAAGAGTTTTCTCGATTATGATAACTCAGAACAGCTATGTGCATTGTAATGTTTGATGGATGAGCTGAAAGAGGCTACTGGGCCCTCTCTCATCTGTATTTTATCGTCTATCATTTGTTTTTTGATTCTACCTTTGTTTATATTCCATTTTTGTTACTCTAAACCCAATCTTATCGCTATTTTTGTCAATGTTTGGATAAGAGTTATAATAAG
TARGET=38,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.5210
PRIMER_LEFT_PENALTY=4.271802
PRIMER_RIGHT_PENALTY=0.249232
PRIMER_LEFT_SEQUENCE=CATGAAAGAGAGATGAGAAAGACAG
PRIMER_RIGHT_SEQUENCE=CTCGGGCTTATTGTTTCAGAAG
PRIMER_LEFT=3,25
PRIMER_RIGHT=297,22
PRIMER_LEFT_TM=58.728
PRIMER_RIGHT_TM=60.249
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=4.8132
PRIMER_LEFT_1_PENALTY=4.271802
PRIMER_RIGHT_1_PENALTY=0.541434
PRIMER_LEFT_1_SEQUENCE=CATGAAAGAGAGATGAGAAAGACAG
PRIMER_RIGHT_1_SEQUENCE=ATGCATCATCGAAGTCAGAGAG
PRIMER_LEFT_1=3,25
PRIMER_RIGHT_1=102,22
PRIMER_LEFT_1_TM=58.728
PRIMER_RIGHT_1_TM=59.459
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=4.8589
PRIMER_LEFT_2_PENALTY=4.271802
PRIMER_RIGHT_2_PENALTY=0.587081
PRIMER_LEFT_2_SEQUENCE=CATGAAAGAGAGATGAGAAAGACAG
PRIMER_RIGHT_2_SEQUENCE=TTTCTCGGGCTTATTGTTTCAG
PRIMER_LEFT_2=3,25
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=58.728
PRIMER_RIGHT_2_TM=60.587
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=4.8645
PRIMER_LEFT_3_PENALTY=4.271802
PRIMER_RIGHT_3_PENALTY=0.592680
PRIMER_LEFT_3_SEQUENCE=CATGAAAGAGAGATGAGAAAGACAG
PRIMER_RIGHT_3_SEQUENCE=TTCCTCTTCAAACGACGAAATC
PRIMER_LEFT_3=3,25
PRIMER_RIGHT_3=202,22
PRIMER_LEFT_3_TM=58.728
PRIMER_RIGHT_3_TM=60.593
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=5.0708
PRIMER_LEFT_4_PENALTY=4.271802
PRIMER_RIGHT_4_PENALTY=0.799006
PRIMER_LEFT_4_SEQUENCE=CATGAAAGAGAGATGAGAAAGACAG
PRIMER_RIGHT_4_SEQUENCE=GAGAAATTGGAAGAAGAATCGG
PRIMER_LEFT_4=3,25
PRIMER_RIGHT_4=270,22
PRIMER_LEFT_4_TM=58.728
PRIMER_RIGHT_4_TM=59.201
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_04589 Ps_contig_mira-and-tgicl-ass_franssen_33837
SEQUENCE=CCTAAGAAGTAACAACTAATACCTCGATAAGAAAAAACCGAAAAAAAAAAACTTAAAATTAATTGACAAATTAGTTTATTTATTCTAACAAAAACAACCAANACCAGAATTAATAAAGAAAACAAACCCATCATAGAATGAAAAACGAAATCTCTGCAATCCTACATTATGCACCGAATTGGGTAGTCTTCCGGGAATATTCAGTAAGCCAATACAGTAGACACAAAAATCCTTCTATAATAATAACAATGTTAATGCCATGTATTTATAGTAACAAAAAAAACCAACAATGTTAACAACAAAAATGTAGAAAAAGGGTTAGTATGACTCTCACTGAGTCTGTTTCCAACTGAAAATAAAGTTAGTGTGAAAAATGTAGAAAAAGGTTTTGTTTCAATAGAAATCTACAACTCAAATTGACATTGAATTATCATTATCAAATCACAGAATCTATAGGTAAAATTGAACTTTAATCACTACATCTCTAAATCACAGAATGTTCATCAATATGTAAAACTATATATCGTAACTGAAGCAATGACTTGAAATAAAATTAAACCATAGTAGCAGCATCATTAAATAAAATTGGTAGCAGTAACTATGTACCTCATAATGTACAGATGTGCAAAATATTTTCAGTGTTCCATACAAAATAAACATCAACAGCAGCATGAGAAAATCGACATTGAAGCTTATGACAAAACATGATATAATCGAAATTTCCTTTCGGAATCTTACCGAACACGAAGATGTTGTTGCAGTTGTTGTCATTATGTTAATTGAAAATGAGTTGTGATGTTATTGTTGCTGTGAACGTCTCATGTACTGCTGAAAAACTAGTGTTGAATTATGCTGCTTGGAAGGGAGATACTGAGTTTGCCGGCGAAATTGAAAACAACATCGGAAACGGGTTCTTGTGCAGTGCAAAAAAAGGATCTCCACTCTTCAAATCTTCGTAGCGAAAATATAGAGATTATTTTGATGAACAAAACAGTGGAATCATGGGTTGGCCGGAAGTGAAGCTCTAGTGGTGGTAACGACATTCGCGGCAGAGGAATTGTGATATCAGAGGGAGTAGTAGTGAAATAGAGGTTTTATCATGGTGGAAAGGCGGAAGAAGGAGATTTCGTGAGGATGAGAAAAGGTGAGGCGGTGAGGTTATGGTAGGCGGAGGTCGTTGGGTTGTGAGGACGCGGAGGGGTGAAGGGTTTGTGGGTGGTTTCCGGCGGCGAGGGAGGGAGACGCGACGGAGATGTAGAGGCGGAAACGAAGGTGGTTGAGAAGGAGGAAACGGCGGTTTCGTGATGGGTTTCGCGGTTGTTGTTGTTGGATAACCGGCGATGGAAGACGGATCGTTGGTTTTGTGGTTGGGTTCGCCGGCGAGGGAGTAGTGAAGGGTGGTGGTTGTGGATTTCCGGCGGTTTGCAGTGGATGAGGAAATGAAGAAAAAGAACAGTGGTGTCTTTTTTCTTTCGTTTTCCTTTCTCTTTTTTTTAGTTTATTTTTTAGCAAAAAAAAAGTGATTATTCAGTCTCCCGCGTACTCTGTCGTGACTACCTACTCGTCCG
TARGET=1317,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2865
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=0.177008
PRIMER_LEFT_SEQUENCE=TTTCGTGAGGATGAGAAAAGGT
PRIMER_RIGHT_SEQUENCE=ACAACCACCACCCTTCACTACT
PRIMER_LEFT=1124,22
PRIMER_RIGHT=1407,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.823
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.2871
PRIMER_LEFT_1_PENALTY=0.110131
PRIMER_RIGHT_1_PENALTY=0.177008
PRIMER_LEFT_1_SEQUENCE=GTGAGGATGAGAAAAGGTGAGG
PRIMER_RIGHT_1_SEQUENCE=ACAACCACCACCCTTCACTACT
PRIMER_LEFT_1=1128,22
PRIMER_RIGHT_1=1407,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=59.823
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.3504
PRIMER_LEFT_2_PENALTY=0.173354
PRIMER_RIGHT_2_PENALTY=0.177008
PRIMER_LEFT_2_SEQUENCE=GAAGAAGGAGATTTCGTGAGGA
PRIMER_RIGHT_2_SEQUENCE=ACAACCACCACCCTTCACTACT
PRIMER_LEFT_2=1113,22
PRIMER_RIGHT_2=1407,22
PRIMER_LEFT_2_TM=59.827
PRIMER_RIGHT_2_TM=59.823
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.3888
PRIMER_LEFT_3_PENALTY=0.211806
PRIMER_RIGHT_3_PENALTY=0.177008
PRIMER_LEFT_3_SEQUENCE=AGGAGATTTCGTGAGGATGAGA
PRIMER_RIGHT_3_SEQUENCE=ACAACCACCACCCTTCACTACT
PRIMER_LEFT_3=1118,22
PRIMER_RIGHT_3=1407,22
PRIMER_LEFT_3_TM=60.212
PRIMER_RIGHT_3_TM=59.823
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.4642
PRIMER_LEFT_4_PENALTY=0.287182
PRIMER_RIGHT_4_PENALTY=0.177008
PRIMER_LEFT_4_SEQUENCE=AAGGAGATTTCGTGAGGATGAG
PRIMER_RIGHT_4_SEQUENCE=ACAACCACCACCCTTCACTACT
PRIMER_LEFT_4=1117,22
PRIMER_RIGHT_4=1407,22
PRIMER_LEFT_4_TM=59.713
PRIMER_RIGHT_4_TM=59.823
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_04590 Ps_contig_mira-and-tgicl-ass_franssen_33841
SEQUENCE=GAAAGGAGCTATGCTTGATTGGGTGAAGAAAATACATCAAGAGAAGAAGATTGATGTGTTAGTTGACAAGGATTTGAAAAACAACTATGACAGGATAGAGCTAGATGGAATAGTTCAAGTTGCTCTTCTATGTACTCAGTACCTTCCGAGCCACAGACCAAAAATGTCAGAAGTAGTTAGAATGCTTGAAGGAGATGGACTTGCAGAGAAATGGGAAGCTTCGCAGAGAGCTGAATCAACAAGAAGTAGAGGAAATGAACTCTCGTCTTCAGAGCGTTATTCTGATCTTACTGATGATTCTTCATTGCTTGCACAAGCAATGGAACTTTCTGGACCAAGATAACATACAAACTCTAGTTTATCAAGACCTTTATTATGCTAATAGGAGACACTCACGTATAGATGTTTGTTTGTTATGGGAAATTTA
TARGET=404,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04591 Ps_contig_mira-and-tgicl-ass_franssen_33843
SEQUENCE=TTTNAANTTCAAAAGAACTCAAATGTCATAATGGAAATAGCCAGTGTGAAGGGAAGTCATCACAGTAAGGCACACTTGTAAACAGTTGGCCAACAAGATGGGAAAATCTAGAGTAATAACAATTAATAATAACCAATTTGCAAATGCAATCCANGGAAGCCAAGCATATGTCTGGCATTTAAAAACCCATTATTAAACTTTAGGTTCTAATTATTTTTCCAGGGCTCTTAAAAGCATAAATAAATAACAAAACATTCAACAAAGGGGTCTAACAAATACCCTAACTTATTGAATCCTCGTGCAGCGCATTCACACCTGCTTGCGTGACAATCAGGTCATGCGATGATGCTATTCACCACAGTTGAGAATACAAACAGGGTATTTACCACCGGTCACCGACAATATATGGCCTGATCAATGAATGGAGGAATTTGTTTTATTTCAGTGCCAAGTTCTTGTTCAATCCTATACAAATTGAAACGATCCTCATAGGTTATTAAGTTCACTGCTAAACCAAGGTGTCCAAACCTCCCTGATCGGCCAACCCTGTGCAGATATGTCTCCGAGTTCTTGGGAAAATCAAAATTAATAACAACATTCACTGCTTGGATATCTATTCCCCTAGTGAAAAGATCAGTACAAACAAGATTTCTGCAGGCACCATTGCGGAAGTCGTGAAAAACTCTATTACGGTGGTCTTGCAACATCTTAGCATGAATATAGAAACATGAATACCCTAGTTCTGTGATTTTCTTGGCAAGGAGTTCAACCCGATTAACCGAATTGCAGAAGATGATTGACTGGTTTATTTGCAGCTTAGAGAAAAGAGTATTTAGGCAGTGGACTTTCTGCCTCTCCTCCACAAATGCATAAAATTGGGTAATACCCTTCAGAGTTAGCTCGTCCATAAGATTAATGATATACGGCTTACGAAGATATCTATCTTTGAAATCCTTTACAGTAACAGGAAATGTAGCTGAAAACATAAGGATTTGACGACTTGAGGGAAGAAATTGAATCAGCTGCTCAATTGAAGGCTGGAACTCTGGGGAGAGAAGTTTATCAGCCTCATCCATCACAAGCATAGAACAATCTTTCAGAACGCAGACACCCTTCTTTGCAAGATCCAAAATTCTTCCCGGAGTACCAACTAGCAGATGGACAGGTTGATATAAACGCATAATGTCGTCTTTCAGGCTTGTACCGCCTGTTGTGACCATAACTTGAATTTGCAAATGTTTTGCAAGCTCTTTACATACTTGAGATGTTTGCAAAGCCAATTCTCGTGTTGGAACCAGTATAACAACTTGAATAACATTGTTACCTTGATCAATTTTTTCCAATGCAGGAACGCAAAATGCAGCTGTTTTGCCTGTTCCATTTTTAGCCCTAGCAAGAATGTCACTACCAGTAAGG
TARGET=236,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0371
PRIMER_LEFT_PENALTY=0.014792
PRIMER_RIGHT_PENALTY=0.022302
PRIMER_LEFT_SEQUENCE=CCAAGCATATGTCTGGCATTTA
PRIMER_RIGHT_SEQUENCE=ACTGTGGTGAATAGCATCATCG
PRIMER_LEFT=159,22
PRIMER_RIGHT=361,22
PRIMER_LEFT_TM=59.985
PRIMER_RIGHT_TM=60.022
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=8.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=0.0498
PRIMER_LEFT_1_PENALTY=0.014792
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=CCAAGCATATGTCTGGCATTTA
PRIMER_RIGHT_1_SEQUENCE=CGGTGGTAAATACCCTGTTTGT
PRIMER_LEFT_1=159,22
PRIMER_RIGHT_1=390,22
PRIMER_LEFT_1_TM=59.985
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.1671
PRIMER_LEFT_2_PENALTY=0.014792
PRIMER_RIGHT_2_PENALTY=0.152322
PRIMER_LEFT_2_SEQUENCE=CCAAGCATATGTCTGGCATTTA
PRIMER_RIGHT_2_SEQUENCE=ATTCCTCCATTCATTGATCAGG
PRIMER_LEFT_2=159,22
PRIMER_RIGHT_2=430,22
PRIMER_LEFT_2_TM=59.985
PRIMER_RIGHT_2_TM=60.152
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.3345
PRIMER_LEFT_3_PENALTY=0.312177
PRIMER_RIGHT_3_PENALTY=0.022302
PRIMER_LEFT_3_SEQUENCE=GCCAACAAGATGGGAAAATCTA
PRIMER_RIGHT_3_SEQUENCE=ACTGTGGTGAATAGCATCATCG
PRIMER_LEFT_3=88,22
PRIMER_RIGHT_3=361,22
PRIMER_LEFT_3_TM=60.312
PRIMER_RIGHT_3_TM=60.022
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.6552
PRIMER_LEFT_4_PENALTY=0.014792
PRIMER_RIGHT_4_PENALTY=0.640429
PRIMER_LEFT_4_SEQUENCE=CCAAGCATATGTCTGGCATTTA
PRIMER_RIGHT_4_SEQUENCE=GCTGCACGAGGATTCAATAAGT
PRIMER_LEFT_4=159,22
PRIMER_RIGHT_4=304,22
PRIMER_LEFT_4_TM=59.985
PRIMER_RIGHT_4_TM=60.640
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_04592 Ps_contig_mira-and-tgicl-ass_franssen_33848
SEQUENCE=AGGTAGTCTAACGNNCRCGRGTCGGGGATTCCCGGACGGAATTCTCTCTCTCTCAAGAACAAACTCTGTTCTTGTTCTCTCTTTCTCTCATTCTCATCAATTAAATTATGTGAGTGAGAATTTCAAACTGTTCAAGATTTCAATTCTGAAAATGGCGGTCACTACTCACTCTCTAACCGCTACAGAAAAGAAACACTGGTGGCTCACTAACCGAAAGATTGTTGAGAAATACTTGAAAGATGCTCGGAACTTAATAGCGACGCAAGAACAGAGCGAGATCGTTTCGGCTTTGAATCTTCTAGATGCGGCTTTAGCTATATCTCCGCGTTTAGATCACGCGCTTGAGTTGAAAGCGAGGTCGTTGCTTTATCTACGGCGGTTCAAGGATGTAGCTGATATGCTTCAAGAATATGTTCCGAGTTTGAGAATGGCGAATGAAGATCCTTCCTCTGGTTCTTCTTCCGAGTCGTCTTCTTCGAGGGAAGGTGTGAAGCTTCTCTCGTCTGATTCGCCGGTGAGAGATCAGAGTTTTAAGTGTTTCTCTGTTTCTGATCTTAAGAAAAAGGTTATGGCAGGTTTATGGAAAAGCTGTGATAAAGAAGGGCAATGGAGATATTTGGTTTTGGGTGAAGCATGTTGCCATTTAGGATTAATGGAGGATGCAATGGTTCTTCTTCAAACTGGAAAGCGGATTGCCAGTGCGGCATTCCGGCGAGAGAGTGTCTGCTGGTCGGACGACAGTTTTCCTCTGTTAACCATCCCGATTGCCGGAGATACGGCGAACCAACCGACCACCTCACCGCCACGAGTTCCTATAAATGTAACTGAAAGAGTAACACATCTCCTCAGCCACATTAAGTTTCTCCTTCGCCGTCGTGCAGCAGCACTTGCTGCACTTGATGCTGGTCTCTACTCGGAAGCCATCCGACATTTCTCAAAAATTGTAGATGGGCGCCGTGCTGCGCCACAAGGCTTCCTTGCGGAGTGTTACATGCATAGAGCCTCTGCAAACCGTTCTGCCGGTCGAATCGCCGAGTCTATAGCTGATTGTAACCGCACCCTTTCTTTAGACCCAACCTGCATTCAAGCCCTTGAAACCAGAGCTTCACTCTTTGAAACAATTCGTTGCTTACCTGATTCTCTACACGATTTAGAACATTTGAAACTTCTTTACAACTCCATCTTACGTGACCGGAAACTTCCTGGTCCGGCATGGAAGCGTCACAATGTCAGATACAGAGAGATTCCAGGGAAACTCTGTGCCTTAACCACCAAGATTCAAGAACTGAAACAAAGGTTAGCTTCTGGTGAAACAACTAGTGTTGATTACTATGCATTGATTGGGATTCGACGAGGTTGTTCGCGGTCCGAATTGGAGAGGGCTCATTTGTTGCTTTGTCTTCGCCATAAACCGGATAAAGCTACAAATTTCATCGAACGGTGTGAGTTTGCAGACGAACGTGATATCGATACAGTTAAGGAGAAAGCAAAGATGTGTTCATTGTTATTGTACAGATTGATTCAAAAGGGTTACACGAGTGTGATGAGTAATGTGTTAGACGAAGAAGCCGCTGAAAAACAAAGAAAGAAAAACTCTGTCGCCGCTGCAGCACAAGCACAAGTGCAGGCACAAGCACAGGCTCAAGCACAAGCACAGGTAGCCGCCATTCAAGCAATTAAGAAAACAAATGAAGCTGAGTTGAAAAACAACAAGCCTGAAGTTAAGATTGGTAATGTTTTAGTTGAAAATGATCAGTCTCAGTCTCTGATATCTTCCTGTACCGTAAACCCTGCTGTGTTTCAAGGTGTGTTTTGCCGCGATCTTGCGGTCGTCGGAAACTTGTTGACGCAGGTGGGATTTAATCGACCGATTCCGGTGAAGTATGAAGCATTGAGCTGCTAAGAAAGTTTTGATGAAAAAAAATTGGTGGGAGGGGCTGTTGGAGATGTAACCGGCAGAGGGAATCTCTGATTCATATTCACTTTTATGATTGTACAAACAAAAATGACTAAAAAAGGG
TARGET=43,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04593 Ps_contig_mira-and-tgicl-ass_franssen_33853
SEQUENCE=TCTACTCATGTATGTTGTTTATCCTTTTCCATGCATGTATGTTGTTGAAACTTTCACTTCCTCTTTGTAAAAAAAGTTATACATTTATTTATTTATTTCTGCATTACCTTATTGCTCTTCCTTTTAATTTGTGCTGCAGAGCCCCTAAATTTCCTTAAAAGGAATTTCTGTCAGAAAATTTCAAACCCTACATTGCCTAAACCATTTCTTTCAAATATATGGTAAACCTCACAAATTAATTTATGAACAAAACTCAACAAAACTTTCTCCTAGAACACATAGAGCATCCATATCAGCTAAATCCGGAAGACAAAATTGGGATTTAGGAAGATTTATTAAAACTTTATACTTCTTCAATGGTCCTCCATCTCCTGCTAAGTTCTTTGACTTTCTAGTTGGGAAACTATCCAGTACTTCCCCTAGTGTATCGGTTAACTCCATGGGGACTTCCGATATTGTTCTTGTTGCTGGAGCTACCGGTGGTGTTGGTCGAAGAGTGGTTGATGTGTTACGGAAGAAAGGAATTCCTGTCCGAGTATTGGTTAGAAATGAAGAGAAAGCAAGAAAGATGTTAGGCTCAGATGTTGACTTGGTTGTTGGAGACATTACAAAAGATAGTACTTTGATCCCTGAATACTTTAAAGGAGTGAAGAAAGTGATCAACGCCGTTTCTGTTATTGTTGGCCCTAAAGAAGGAGACACTCCAGACAGAGCGAAATACAGCCAAGGAATTAAGTTCTTCGAGCCAGAGATAAAAGGCGATTCACCAGAAAAGGTAGAGTACATAGGAATGAGAAATTTGATCAAGGCCGTGAAGAACAACCTTGGACTTCGAAAGGGAAAGCTATTATTTGGATTTGAAGGCGAAAGTTACAGGCAGCTTTCTTGGGGCGCTTTAGATGATGTGGTAATGGGTGGAGTTAGTGAAAGTACTTTTCAGATTGATTCAAATGGTAGTGAAAATGGTGGACCAACCGGGGTTTTTAAAGGTGTTGTTTCATCGGCAAATAATGGTGGCTTTACAAGTATCAGAACAAAGAATTTTTCAGAACCTGAGGATCTCTCTGCATATGATGGTTTGGAGTTCCGTCTAAAAGGTGATGGGCGTAGATATAAGGTCATTATACGCACAAGCCCTGATTGGGATGCTCTTGGGTACACTGCAGGCTTTAACACAGAGAAAGGCAAATGGCAATCGATTCAATTGCCATTTTCTTCCCTGAGGCCCATATTTCGAGCAAGGACTGTGTCTGATGCTCCGCAATTTGATGCCAGCAACATTGCATCACTGCAGCTCATGTTCAGCAAGTTTGAATATGATGGTAAATTAAACGAGACTTTTGCGGAAGGTCCGTTTGAGCTTCCAGTGTCTAGCATAAAGGCATATATAAATGATCCAATAACTCCTAGATTTGTACATGTGGGCTCAGCGGGAGTTACCAGACCGGAAAGGCCCGGACTTGATCTAAGTAAACAGCCTCCTGCAGTTCGATTAAACAAGGAACTGGATTATATCCTCACATTTAAACTAAAGGGTGAGGATTCAATCCGAGAAAGTGGCATTCCATATACGATCGTTAGGCCTTGTGCGCTAACTGAAGAACCTGCTGGAGCTGATCTAATCTTTGATCAAGGAGATAATATTACGGGTAAAATATCAAGGGAAGAGGTTGCTCAAATGTGCGTAGCTGCACTTCAAAGCCCTTATGCTTGTGATAAAACATTTGAGGTGAAAAGTGTTATTCCGTTTAGCGAGCCATTTACGGTGGATCCCGAAAATCCTCCTCCAGAGAAGGACTATGACATATACTTTAAAAGTTTAAAAGAAGGCATAACTGGGAAGGAAGCCCTCCAACAAAATCCTATACCTGTTTAATGTTCTTGCATTGCAGTTCAGCTATGGATATTGAGGATATATATAAAAGTACATTGAGAGCTGCACATAATCTTTGAATCCAAAGAAATATAAACCAAACAAAACTATGATATTTCATAAGCCTAGACATGAATGTTGCCCTATACAAGATGCATGTATATTCATTGTACTTAAACATACTTTCCTTTTCCTTTGTTTGTTTTAGTCATATAGGAAGGTAAAGAATAACCACACCACACTTTTCATATACCAAAATAAATTTGGTTTTGACTCCAATTTTGAGGTT
TARGET=83,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2938
PRIMER_LEFT_PENALTY=0.243575
PRIMER_RIGHT_PENALTY=0.050241
PRIMER_LEFT_SEQUENCE=CCTTTTCCATGCATGTATGTTG
PRIMER_RIGHT_SEQUENCE=CTTCCGGATTTAGCTGATATGG
PRIMER_LEFT=22,22
PRIMER_RIGHT=308,22
PRIMER_LEFT_TM=60.244
PRIMER_RIGHT_TM=59.950
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.3390
PRIMER_LEFT_1_PENALTY=0.243575
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=CCTTTTCCATGCATGTATGTTG
PRIMER_RIGHT_1_SEQUENCE=TTGTCTTCCGGATTTAGCTGAT
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=60.244
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=291
PRIMER_PAIR_PENALTY_2=0.4506
PRIMER_LEFT_2_PENALTY=0.243575
PRIMER_RIGHT_2_PENALTY=0.206979
PRIMER_LEFT_2_SEQUENCE=CCTTTTCCATGCATGTATGTTG
PRIMER_RIGHT_2_SEQUENCE=TCCGGATTTAGCTGATATGGAT
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=306,22
PRIMER_LEFT_2_TM=60.244
PRIMER_RIGHT_2_TM=59.793
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.4709
PRIMER_LEFT_3_PENALTY=0.243575
PRIMER_RIGHT_3_PENALTY=0.227281
PRIMER_LEFT_3_SEQUENCE=CCTTTTCCATGCATGTATGTTG
PRIMER_RIGHT_3_SEQUENCE=GGTTTAGGCAATGTAGGGTTTG
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=202,22
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=59.773
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.4709
PRIMER_LEFT_4_PENALTY=0.243575
PRIMER_RIGHT_4_PENALTY=0.227281
PRIMER_LEFT_4_SEQUENCE=CCTTTTCCATGCATGTATGTTG
PRIMER_RIGHT_4_SEQUENCE=TGGTTTAGGCAATGTAGGGTTT
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=203,22
PRIMER_LEFT_4_TM=60.244
PRIMER_RIGHT_4_TM=59.773
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_04594 Ps_contig_mira-and-tgicl-ass_franssen_33856
SEQUENCE=ACGAATATTAATCCACCAGCAATAATCATTCATATAATTATTATTATCATCATCATGATTTCTATGTTCAATCGTCTCTTATCTCTCTCTATACACCAAAATGAAATGCAAAAGAGATGATTAAAACATTTTTCTTTTATGACAAAATCGGATTTCATAGTGTCGCCGTGATTTTGTCAAACTAAACACGCACTAAAATGAGGCGGCTTCTTTTGATATGCGGTGGTGTCGTTTTCCGCGGCGGTGCCTTCCGTTACCGAATGTGACGGGATCGAATTTTCTTAGTTTTTCGGCTTGTCGCGAATCGATCACGCACTCGGGTAGAGGGACTTTGTATCTGGTTTTGTCGTAACAGTAAGAATATGTCATGTGCTTTAACCTAAAGTTGTCCATTCTGATTTTTTCGACATGTGTTACATCAGAAGAAGGAAGTGTTTCGGAAGTTTGAAGACTGTTGTCGCAGTTAGTCACATGTTCAATAGGATCAACTGCGCAACCATGTAGGACAAAGTCTGAGAATTCAGCAACAAAAGGTGCATATTTGTAGTTTACTTTGTATTTTCCTCCATTGGTTGCCCAAGTAGATGCATCCCATATGGTTGAATACAATGTCATTGGTTTTGATGGGAAATCTCCTCCCATTGATTCTGTTCTCTTGATTTCTCTTATAGGAACTTCGTCTACATAAAATATGATCTTAGAATCTGTCCAGAGAATGCTGTAGTGATGAACATCCTCAGAAGGATCAAACCATAGACCATATCTTTCTTCTCTTCCAATGTTTGTACTTCCATTTCCATAAATATTGGTTTGAATCCTCCAATCTTTGCCTCTTATGTTTCCCAAAAATTCAAAGTCTATTTCATCATGGTTCTTCTCATACATGTCACCATTTGATAAATAGAATGCAACAACAACTCCAGCAGTGTAATCAGAAGGTAATTTAATGGAAGCACTGAAATACCCATGAAGGTAAATATCATGAGAAGCAAATCCAGAGCCTGAAGACTCATCAAGAGAGAGATGAACAGTTTGGGAATCAGAATGAACGATCAAGTTATGGTCTCCAAACAAAGGTGAATAGCCTTCATCGAAGGGTATAATAGGAAAAACTCTGGAGGATGATGAGACAGAAGAAGAAAGTAGAAGAAAGAGAAAAGAACGGAAGAAGAAGATGAAAAAGAACGTTCCCATGTTAATTGTGGAGGGTAAGGAAAATTAATAAATAAAATGAATAATAATAATAAAGAGAGAAGAGAGAGTGAAGAAGAAGAAGAGAAATGGAAAGGAATATAACAAGAGGAGAGGAGTGAGGAGATTTTGGGATCCCCGNA
TARGET=1234,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1263
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.125540
PRIMER_LEFT_SEQUENCE=CTCCAAACAAAGGTGAATAGCC
PRIMER_RIGHT_SEQUENCE=TCTCCTCACTCCTCTCCTCTTG
PRIMER_LEFT=1064,22
PRIMER_RIGHT=1317,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.126
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.1263
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.125540
PRIMER_LEFT_1_SEQUENCE=TCCAAACAAAGGTGAATAGCCT
PRIMER_RIGHT_1_SEQUENCE=TCTCCTCACTCCTCTCCTCTTG
PRIMER_LEFT_1=1065,22
PRIMER_RIGHT_1=1317,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.126
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.3173
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.316570
PRIMER_LEFT_2_SEQUENCE=CTCCAAACAAAGGTGAATAGCC
PRIMER_RIGHT_2_SEQUENCE=ATCCCAAAATCTCCTCACTCCT
PRIMER_LEFT_2=1064,22
PRIMER_RIGHT_2=1326,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.317
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=0.3173
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.316570
PRIMER_LEFT_3_SEQUENCE=TCCAAACAAAGGTGAATAGCCT
PRIMER_RIGHT_3_SEQUENCE=ATCCCAAAATCTCCTCACTCCT
PRIMER_LEFT_3=1065,22
PRIMER_RIGHT_3=1326,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.317
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.4779
PRIMER_LEFT_4_PENALTY=0.352395
PRIMER_RIGHT_4_PENALTY=0.125540
PRIMER_LEFT_4_SEQUENCE=AGTTTGGGAATCAGAATGAACG
PRIMER_RIGHT_4_SEQUENCE=TCTCCTCACTCCTCTCCTCTTG
PRIMER_LEFT_4=1029,22
PRIMER_RIGHT_4=1317,22
PRIMER_LEFT_4_TM=60.352
PRIMER_RIGHT_4_TM=60.126
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_04595 Ps_contig_mira-and-tgicl-ass_franssen_33856
SEQUENCE=ACGAATATTAATCCACCAGCAATAATCATTCATATAATTATTATTATCATCATCATGATTTCTATGTTCAATCGTCTCTTATCTCTCTCTATACACCAAAATGAAATGCAAAAGAGATGATTAAAACATTTTTCTTTTATGACAAAATCGGATTTCATAGTGTCGCCGTGATTTTGTCAAACTAAACACGCACTAAAATGAGGCGGCTTCTTTTGATATGCGGTGGTGTCGTTTTCCGCGGCGGTGCCTTCCGTTACCGAATGTGACGGGATCGAATTTTCTTAGTTTTTCGGCTTGTCGCGAATCGATCACGCACTCGGGTAGAGGGACTTTGTATCTGGTTTTGTCGTAACAGTAAGAATATGTCATGTGCTTTAACCTAAAGTTGTCCATTCTGATTTTTTCGACATGTGTTACATCAGAAGAAGGAAGTGTTTCGGAAGTTTGAAGACTGTTGTCGCAGTTAGTCACATGTTCAATAGGATCAACTGCGCAACCATGTAGGACAAAGTCTGAGAATTCAGCAACAAAAGGTGCATATTTGTAGTTTACTTTGTATTTTCCTCCATTGGTTGCCCAAGTAGATGCATCCCATATGGTTGAATACAATGTCATTGGTTTTGATGGGAAATCTCCTCCCATTGATTCTGTTCTCTTGATTTCTCTTATAGGAACTTCGTCTACATAAAATATGATCTTAGAATCTGTCCAGAGAATGCTGTAGTGATGAACATCCTCAGAAGGATCAAACCATAGACCATATCTTTCTTCTCTTCCAATGTTTGTACTTCCATTTCCATAAATATTGGTTTGAATCCTCCAATCTTTGCCTCTTATGTTTCCCAAAAATTCAAAGTCTATTTCATCATGGTTCTTCTCATACATGTCACCATTTGATAAATAGAATGCAACAACAACTCCAGCAGTGTAATCAGAAGGTAATTTAATGGAAGCACTGAAATACCCATGAAGGTAAATATCATGAGAAGCAAATCCAGAGCCTGAAGACTCATCAAGAGAGAGATGAACAGTTTGGGAATCAGAATGAACGATCAAGTTATGGTCTCCAAACAAAGGTGAATAGCCTTCATCGAAGGGTATAATAGGAAAAACTCTGGAGGATGATGAGACAGAAGAAGAAAGTAGAAGAAAGAGAAAAGAACGGAAGAAGAAGATGAAAAAGAACGTTCCCATGTTAATTGTGGAGGGTAAGGAAAATTAATAAATAAAATGAATAATAATAATAAAGAGAGAAGAGAGAGTGAAGAAGAAGAAGAGAAATGGAAAGGAATATAACAAGAGGAGAGGAGTGAGGAGATTTTGGGATCCCCGNA
TARGET=1264,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1263
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.125540
PRIMER_LEFT_SEQUENCE=CTCCAAACAAAGGTGAATAGCC
PRIMER_RIGHT_SEQUENCE=TCTCCTCACTCCTCTCCTCTTG
PRIMER_LEFT=1064,22
PRIMER_RIGHT=1317,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.126
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.1263
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.125540
PRIMER_LEFT_1_SEQUENCE=TCCAAACAAAGGTGAATAGCCT
PRIMER_RIGHT_1_SEQUENCE=TCTCCTCACTCCTCTCCTCTTG
PRIMER_LEFT_1=1065,22
PRIMER_RIGHT_1=1317,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.126
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.3173
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.316570
PRIMER_LEFT_2_SEQUENCE=CTCCAAACAAAGGTGAATAGCC
PRIMER_RIGHT_2_SEQUENCE=ATCCCAAAATCTCCTCACTCCT
PRIMER_LEFT_2=1064,22
PRIMER_RIGHT_2=1326,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.317
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=0.3173
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.316570
PRIMER_LEFT_3_SEQUENCE=TCCAAACAAAGGTGAATAGCCT
PRIMER_RIGHT_3_SEQUENCE=ATCCCAAAATCTCCTCACTCCT
PRIMER_LEFT_3=1065,22
PRIMER_RIGHT_3=1326,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.317
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.4779
PRIMER_LEFT_4_PENALTY=0.352395
PRIMER_RIGHT_4_PENALTY=0.125540
PRIMER_LEFT_4_SEQUENCE=AGTTTGGGAATCAGAATGAACG
PRIMER_RIGHT_4_SEQUENCE=TCTCCTCACTCCTCTCCTCTTG
PRIMER_LEFT_4=1029,22
PRIMER_RIGHT_4=1317,22
PRIMER_LEFT_4_TM=60.352
PRIMER_RIGHT_4_TM=60.126
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_04596 Ps_contig_mira-and-tgicl-ass_franssen_33858
SEQUENCE=ACCGCAAAGGAGGCACAGAGAAAAAAAAGAGCTCAACTCCCAAGTCCCAACCAGAGGCATAGCATTTCATCAATGGCCTCAATGTTCTTGAGACCCATCCTCATCACTCTCTTCACTACTTTCCTCATCTCTCACATTTCAGTACTGAAGGTATCAGCAGCAACAGTAATATTTTCCAACAAATGTCCGCATCCAGTATGGCCAGCCATCCAGCCCGGCGCAGGAAAGCCCATACTAGCCCGCGGCGGCTTCAACCTCCAGCCTAACAAAGCCTACTCTCTCCAAGTCCCACCTCTCTGGTCAGGCCGTTTCTGGGGCCGTCACGGCTGCACCTTCGACGCATCAGGACACGGCCATTGCGCCACCGGAGACTGCGGCGGATCTCTCTTTTGCAACGGCATCGGAGGAACACCACCAGCGACCCTCGCCGAGTTCACACTCGGAAAAGATCTAGATTTCTACGACGTGAGTCTCGTCGACGGTTACAACTTACCGATTTCCATTACTCCTATAAAGGGATCCGGTAAGTGTAGCAACGCCGGTTGTGTGAGTGATCTGAACTTGATGTGTCCGGTGGGCTTGCAAGTGAGGTCACGCGATAAGCGCGTGGTGGCTTGTAAGAGTGCTTGTTCTGCTTTTAACTCGCCTAGGTATTGCTGTACTGGTAGCTTTGGAAGCCCACAGGCCTGTAAGCCCACTGCTTATTCTAGAATCTTCAAAACTGCTTGCCCAAAAGCCTACTCTTATGCCTATGATGACCCTACCAGTATTGCTACTTGCACCAATGCTAACTATTTGATCACTTTCTGCCCTCATCACCACCGTTGATTTATTACTAGTAGTAGGTTAATGTCATATTGAGTATTAATGTGTTACCGTTAATGTCTTTGTGTTTATTTTATGTGATGATGGATAGTTTATGTTTGGTAGTAGTAGTATATCTACTAGGTCTTTTTTTAATGTAGTACTATATTTAGTAACTCTTATTATTATTTATGGTTGGTTAAAGCCTANTTTGGTTCTTAATTTATCCTTTCTACTAATGGTGGTGGGATTAGATTCGGGATGGTTTCGAATTCACACATTTACCGATACTGGTTATTTGAACG
TARGET=927,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5444
PRIMER_LEFT_PENALTY=0.301100
PRIMER_RIGHT_PENALTY=0.243298
PRIMER_LEFT_SEQUENCE=ACCAGTATTGCTACTTGCACCA
PRIMER_RIGHT_SEQUENCE=CCCACCACCATTAGTAGAAAGG
PRIMER_LEFT=762,22
PRIMER_RIGHT=1052,22
PRIMER_LEFT_TM=59.699
PRIMER_RIGHT_TM=59.757
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.5692
PRIMER_LEFT_1_PENALTY=0.325886
PRIMER_RIGHT_1_PENALTY=0.243298
PRIMER_LEFT_1_SEQUENCE=TGCTACTTGCACCAATGCTAAC
PRIMER_RIGHT_1_SEQUENCE=CCCACCACCATTAGTAGAAAGG
PRIMER_LEFT_1=770,22
PRIMER_RIGHT_1=1052,22
PRIMER_LEFT_1_TM=60.326
PRIMER_RIGHT_1_TM=59.757
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=1.0406
PRIMER_LEFT_2_PENALTY=0.797267
PRIMER_RIGHT_2_PENALTY=0.243298
PRIMER_LEFT_2_SEQUENCE=TTGCTACTTGCACCAATGCTAA
PRIMER_RIGHT_2_SEQUENCE=CCCACCACCATTAGTAGAAAGG
PRIMER_LEFT_2=769,22
PRIMER_RIGHT_2=1052,22
PRIMER_LEFT_2_TM=60.797
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=284
PRIMER_PAIR_PENALTY_3=1.1632
PRIMER_LEFT_3_PENALTY=0.193532
PRIMER_RIGHT_3_PENALTY=0.969648
PRIMER_LEFT_3_SEQUENCE=ATTGCTACTTGCACCAATGCTA
PRIMER_RIGHT_3_SEQUENCE=CCAAANTAGGCTTTAACCAACC
PRIMER_LEFT_3=768,22
PRIMER_RIGHT_3=1018,22
PRIMER_LEFT_3_TM=59.806
PRIMER_RIGHT_3_TM=59.030
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.50
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.75
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=1.1632
PRIMER_LEFT_4_PENALTY=0.193532
PRIMER_RIGHT_4_PENALTY=0.969648
PRIMER_LEFT_4_SEQUENCE=TATTGCTACTTGCACCAATGCT
PRIMER_RIGHT_4_SEQUENCE=CCAAANTAGGCTTTAACCAACC
PRIMER_LEFT_4=767,22
PRIMER_RIGHT_4=1018,22
PRIMER_LEFT_4_TM=59.806
PRIMER_RIGHT_4_TM=59.030
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.50
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.75
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_04597 Ps_contig_mira-and-tgicl-ass_franssen_33864
SEQUENCE=CCTTTTGGAACTCAAACTTGTCAAGCTTTCATTCAATAAAGACAAATAACAAAACAACTGTAACTAGTAAAAGGAATGATAATATTATATCTACAAGATCAGAAAAGAGAAAATCTATCATCTCATTTCTGTAAAAACTTTGGCCATTTGTCGCGGTGCAACTAAAATCTTAATAATAACAATTGCACACACACAAAAAAAGGTTTGAAAATATTTATATATTACAATGTTTGGAAGACCACTTTGTTGTGTATTTTCTTGTATGTTCTTTCACAACAGTGGAATGGACAATTCAATCTTGGTGAAGTTCGTCAGCTTGTGGGGCAGCAAATCTAACATTGGCAGGTCGTGCCACAACAACTGGGTCATCAGCAAATAGCGCTTTGATGAGTGATGGTGTTTTTAGGGGCCTACGATTAGTCATCCTAGGATAGACATCTTCTAGAAAATAGTATGCATGGCCCGCAATCATTCCCAGTAGGTCTACCCAGGCACTAGCACCAACAAGGACAGAGAACCCCAAGAGAACCCATGGAAGGTAGGCGGCGGTGAAAGTAAAAAGACCTAGGAAGCTCATGTGAATATAGGGATTCTGTTTGCTCCAAACATAAACCATCATAAATGTCAAGGAGTTGCTGAGGAATATAATTTTTGCAAATGACTCTGATAAGTAAGGTATCATTCCACCGAGAAGAACGATTCCAGTCAATACAGATGCACCAAATAAGAGCATGTAGAAAAAATCAGCAGTTCTTCCCCTGAATGAATTCTCTTCTAGAAGCTTGCAGTACCGAGCAAGAAAAAACATATGAAACAGGAAATCCAAGTCCATCTTCCTGAAGTACAAGAAATTAGTAACCAAACGCCAAAACTGATATTGCTTCACAACCAGTCTAGGGTTCAAGTACAAATGATAAGGAGAAATTATCTCGAGGGAGCATCCGATGGTGGTGACAACAGCCGCAGTGAGATAGGATCTTGTAATAACCGGCATCTGTTTGTACCATTCTTCAACCGCTTGAGCCATTGTTGCTTTTTACCCTAATCTCTGCCTCTGATCCTAACTAAACTTTATAATAATAAGAAGAAGAACGATATGAAAAGAAAGTGAGTGTTTGGGTGTGATGCTTCAGGAAAAGTGGGAACGAAAACGAAAATGTGAGATTTGAGGGGTGTTTGATGTCATCGACGACTCTGCGTTTGTATACCACTGCTT
TARGET=186,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0334
PRIMER_LEFT_PENALTY=2.025128
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=AAAACTTTGGCCATTTGTCG
PRIMER_RIGHT_SEQUENCE=CCATTCCACTGTTGTGAAAGAA
PRIMER_LEFT=133,20
PRIMER_RIGHT=286,22
PRIMER_LEFT_TM=59.975
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=2.0414
PRIMER_LEFT_1_PENALTY=2.025128
PRIMER_RIGHT_1_PENALTY=0.016290
PRIMER_LEFT_1_SEQUENCE=AAAACTTTGGCCATTTGTCG
PRIMER_RIGHT_1_SEQUENCE=TGACTAATCGTAGGCCCCTAAA
PRIMER_LEFT_1=133,20
PRIMER_RIGHT_1=422,22
PRIMER_LEFT_1_TM=59.975
PRIMER_RIGHT_1_TM=59.984
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=2.0702
PRIMER_LEFT_2_PENALTY=2.025128
PRIMER_RIGHT_2_PENALTY=0.045112
PRIMER_LEFT_2_SEQUENCE=AAAACTTTGGCCATTTGTCG
PRIMER_RIGHT_2_SEQUENCE=CAACAAAGTGGTCTTCCAAACA
PRIMER_LEFT_2=133,20
PRIMER_RIGHT_2=248,22
PRIMER_LEFT_2_TM=59.975
PRIMER_RIGHT_2_TM=60.045
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=2.0702
PRIMER_LEFT_3_PENALTY=2.025128
PRIMER_RIGHT_3_PENALTY=0.045112
PRIMER_LEFT_3_SEQUENCE=AAAACTTTGGCCATTTGTCG
PRIMER_RIGHT_3_SEQUENCE=CACAACAAAGTGGTCTTCCAAA
PRIMER_LEFT_3=133,20
PRIMER_RIGHT_3=250,22
PRIMER_LEFT_3_TM=59.975
PRIMER_RIGHT_3_TM=60.045
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=2.1137
PRIMER_LEFT_4_PENALTY=2.105486
PRIMER_RIGHT_4_PENALTY=0.008257
PRIMER_LEFT_4_SEQUENCE=CCTTTTGGAACTCAAACTTGTC
PRIMER_RIGHT_4_SEQUENCE=CCATTCCACTGTTGTGAAAGAA
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=286,22
PRIMER_LEFT_4_TM=57.895
PRIMER_RIGHT_4_TM=60.008
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_04598 Ps_contig_mira-and-tgicl-ass_franssen_33872
SEQUENCE=AAGAATGTGAAGTCACAAAGACTGACTCTGACTCTTGTCTTCTTTCTTCCTCTTTCGTTGTTCCGAACCCAACACAGTACAACCTCAACAATGTCGAATTCCAGCAACCCACCACCACCAAAACCCACATGGGTTCTCCCTTACGTAACAGAGAATCTCCGTGAACTCTACACCTTAGGTAGAAAACTGGGTCAAGGTCAATTCGGCACCACCTATCTCTGCACACACAACCCAACCGGTCGAACCTACGCTTGCAAATCAATCCCAAAGAAGAAGCTTCTATGCAAAGAGGATTACGATGATGTGTGGAGAGAGATTCAGATAATGCACCATTTATCTGAGCATCCTAATGTTGTCAGGATCCATGGGACTTATGAGGATTCGTTTTCTGTTCATTTGGTTATGGAGCTTTGTGAAGGTGGTGAGTTGTTTGATAGGATTGTGAAGAAGGGGCATTATAGTGAGAGGGAAGCTGCTAAGTTGATTAGAACTATTGTTGAGGTTGTTGAAAATTGTCATTCTCTTGGTGTTATGCATAGAGACCTTAAGCCTGAGAATTTTTTGTTTGATACTGTTGATGAAGATGCTGTTCTCAAAACTACTGATTTTGGGTTGTCTGCTTTTTACAAGCCAGGTGAAATCTTTAGTGATGTTGTTGGAAGCCCATACTATGTTGCACCAGAGGTTTTGCACAAGCATTATGGACCCGAGGCTGATGTGTGGAGTGCCGGGATTATTTTGTACATCTTATTAAGCGGGGTGCCGCCATTTTGGGCTGAGACAGAACAAGGGATCTTCAGACAGATTTTGCAGGGAAGACTTGATTTCCAGTCTGAGCCATGGCCTGGGATTTCAGACAGCGCCAAGGATCTAATTCGAAAAATGCTTGATAGGAATCCGAAAAATAGGTTTACAGCTCACCAAGTGCTATGTCATCCATGGATTGTCGATGATAACATTGCACCAGACAAACCTCTTGATTCGGCTGTTTTAACTCGCTTGAAGCAGTTCTCTGCAATGAATAAACTTAAAAAGATGGCTTTACGTGTTATTGCGGAGATGCTGTCCGAGGAAGAAATTGGCGGTTTGAAAGAGTTATTCAGGATGCTTGATGCTGACAGTAGTGGAACCATAACATTGGATGAGCTAAAAGAAGGCTTAAAGCGAGTAGGATCTGAACTTATGGAATCTGAAATCAAGGATCTTATGGATGCAGCAGATGTTGATAATAACGGCACGCTCGACTACGGTGAATTCATTGCTGCTACTGTCCACTTAAACAAGCTGGAAAGAGAGGAAAATCTCTTGTCTGCATTCTCCTACTTTGACAAAGATGGAAGTGGTTACATAACCATTGACGAGATTCAAGTAGCTTGTAAGGAGTTCGGTTTGGATGATATCCATATCGATGAAATGGTCAAGGAAATTGATCAAGATAATGATGGACAAATAGATTATGGAGAATTTGCTGCCATGATGAGAAAGGGCAATGGCGGGATGGGAAGACGAACCATGACAAGCAGACTCAATTTCAGAAATGCTCTTGGAATCATAGGCAATGGGTCCAATTAAATTATTGTACCTCAAATAACTATTTAGTGATTATAAACTATAGTAGCTTTCAACATTATCAGTAACAACCAAAATTTGTCTTTGTAAACAGACCCAAAATTGGAGATAAAAAATGCATTTAAGTTTAAGAAATTAATT
TARGET=109,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4235
PRIMER_LEFT_PENALTY=0.258908
PRIMER_RIGHT_PENALTY=0.164639
PRIMER_LEFT_SEQUENCE=TTTCTTCCTCTTTCGTTGTTCC
PRIMER_RIGHT_SEQUENCE=GAAGCTTCTTCTTTGGGATTGA
PRIMER_LEFT=42,22
PRIMER_RIGHT=279,22
PRIMER_LEFT_TM=59.741
PRIMER_RIGHT_TM=59.835
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.4391
PRIMER_LEFT_1_PENALTY=0.258908
PRIMER_RIGHT_1_PENALTY=0.180233
PRIMER_LEFT_1_SEQUENCE=TTTCTTCCTCTTTCGTTGTTCC
PRIMER_RIGHT_1_SEQUENCE=TCCACACATCATCGTAATCCTC
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=309,22
PRIMER_LEFT_1_TM=59.741
PRIMER_RIGHT_1_TM=59.820
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.4391
PRIMER_LEFT_2_PENALTY=0.258908
PRIMER_RIGHT_2_PENALTY=0.180233
PRIMER_LEFT_2_SEQUENCE=TTTCTTCCTCTTTCGTTGTTCC
PRIMER_RIGHT_2_SEQUENCE=TCTCCACACATCATCGTAATCC
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=311,22
PRIMER_LEFT_2_TM=59.741
PRIMER_RIGHT_2_TM=59.820
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=270
PRIMER_PAIR_PENALTY_3=0.4966
PRIMER_LEFT_3_PENALTY=0.258908
PRIMER_RIGHT_3_PENALTY=0.237699
PRIMER_LEFT_3_SEQUENCE=TTTCTTCCTCTTTCGTTGTTCC
PRIMER_RIGHT_3_SEQUENCE=TTACGTAAGGGAGAACCCATGT
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=147,22
PRIMER_LEFT_3_TM=59.741
PRIMER_RIGHT_3_TM=59.762
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.4966
PRIMER_LEFT_4_PENALTY=0.258908
PRIMER_RIGHT_4_PENALTY=0.237699
PRIMER_LEFT_4_SEQUENCE=TTTCTTCCTCTTTCGTTGTTCC
PRIMER_RIGHT_4_SEQUENCE=TGTTACGTAAGGGAGAACCCAT
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=149,22
PRIMER_LEFT_4_TM=59.741
PRIMER_RIGHT_4_TM=59.762
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_04599 Ps_contig_mira-and-tgicl-ass_franssen_33875
SEQUENCE=CGGCTTAGCACACCGCCATGGCTACAGCTTCTGCTTYCGGGGATCCTTCACTACCAAATATCACATTTGAGTACAATGGTTTCCAAAAATGCAATCCTCGTACTTGGCCTATTGGCCATGGTTGTTCTTATTTCATCAGAGGTGTCAGCTAGGGACTTAGTAGAGACTTCCACTAATGCCAAAGAGGAGGTTGTTGAAAAGTCAAATGAAATAAATGATGCCAAATATTATGGTGGTGGCTACCACAATGGATATGGTGGTTACCATGGCGGTGGTGGAGGTTACCACAATGGCTATGGTGGTTACCATGGTGGTGGTGGTTACCATGGTGGTTATAATGGTGGTTATGGTGGTTATCATGGTGGTGGAGGTGGTTATGGAGGAGGTTACGGTCATCATGGTGGTGGTTACAATGGTGGTGGAGGAGGTTACGGTCATGGTGGAGGTGGTTACAACGGTGGTGGTTTTGACGATCGTAACTGATTATATCATGATGCATGCATGTATTAAGTGTAATAAATTTCATGAAGTAAATAATGTTGGGGTTGAAGAAAAGAGGTATAGATAGACATCCTTTGTGGTTATGTATTATCTATCTTACCTGTCTATAGTAGAGATATTTTACGACTCTATAAATCACTATGTCTTTCATGTGTAATATATCAAATAAAGGTTAGTTATTGGAATTTTGTCTTTTRTGTCTGTTTTTGATGGTTTGTTTGGCATAACTGTGTAATATATCAAATCACGGGTGTCTGCGTTGCGGTTTTGATTAGG
TARGET=309,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04600 Ps_contig_mira-and-tgicl-ass_franssen_33880
SEQUENCE=TCTTNTAGTTTCTAGTGTTTAGTTCACTCAACCCAGATACAAAGCTTCAATCTTTCTCCTCTTTCTTGAAATTGGGTGATGGAATTCAACTGAAGAGAGAAACTGTGTGCAAGAATATGGCTTATCTACAAGGGTTTACTCCAACTTTGAAATTGGGTTTTGTGTTTTCATCGCTAGTTTCACCAAAACAGAGACACCCTTTGGTTTTCCCTTCATCAAAGTGTGGATTTTCCCTCAAATTCAATGATGGGTCATTCAAATTTCAAAACCCTTTAAATTAAAGGGAACAAAATCGGTGGAGCTTGACCGGTTTGTTACCAGTGACGACGAAGAGGAAGAAGAAGAAATGGGAGATGGGTTCTTGGAAGCAATAGAGGAGCTTGAGAGAATGACAAGGGAGCCTTCTGATGTTCTTGAAGAGATGAACGATCGTCTCTCAGCTAGAGAGCTTCAGCTTGTGTTGGTTTATTTCTCTCAAGATGGAAGAGACTCGTGGTGTGCTTTGGAGGTTTTTGATTGGTTAAGGAAAGAGAATAGGGTTGATAAAGAGACTATGGAGTTAATGGTTGCTATCATGTGTGGTTGGGTGAAGAAGTTGATTCAGGAACAGCATGGGGTTGGTGATGTCGTTGACCTTCTTGTGGATATGGATTGTGTTGGGTTGAGGCCTGGTTTTAGTATGATTGAGAAGGTTATTTCTTTGTATTGGGATATGGGGGAGAAAGAAGGTGCTGCTTTGTTTGTGGAAGAGGTTTTGAGGCGTGGAGTTTCTTGGAATGAGGGTGATCCTCAAGGGCATAAAGGAGGCCCAACTGGGTATCTTGCTTGGAAGATGATGGCTGAGGGTGATTACAAAGGTGCTGTTCGGTTGGTAATTCGGTTTAGAGAAGCTGGTTTGAAGCCAGAGATCTACAGTTACCTTGTTGCAATGACTGCCATAGTTAAGGAGCTCAACGAGTTTGCCAAAGCTCTACGAAAGTTGAAAAGTTTCGCTAGGGCCGGGCTTGTTACCGAATTCGACCGAGAAGATGTTGAGCTAGCAGAGAACTACCAATCAGAACTTCTAGCCGACGGAGCCCGCTTATCCAACTGGGTGATTCAAGACGGCAGTCATCCATCACTTCACGGAGTTATCCACGAGAGGCTCCTCGCAATGTACATCTGCGCCGGCCGTGGACTCGAAGCCGAGAAACAATTATGGGAAATGAAACTTGTTGGCAAAGAAGCCGACAGAGGTCTCTACGACATTGTTCTAGCCATATGTGCTTCTCAGAAAGAAGCTGCAGCGACAGGAAGGCTAATGACGCAGTTGGAATTTGCGAGCTCGGCGCAGAAGAAGAAAAGTCTGTCATGGTTGTTAAGAGGATACATTAAAGGTGGTCATTTCAATGAAGCAGCAGAGACAGTGATGAAAATGCTTGAATTGGGAATTTGTCCTGAGTATTTGGATAGAGTTGCTGTTGTTCAAGGTTTAAGGAAAAGGGTTCATCAAAATGGAAATTTAGATACTTATTTTAAGCTTTGTAAGTCACTTTCTGAAGCAAATCTTATAGGGCCTTGTCTTGTGTATTTGTATACAAGAAAATATAAGCTTTGGGTTGTGAAAATGGTGTAAGAAATGTAGTAGAGAGAAACTGGTGGTTATTACTTGTATAAATTTGTATAGTTTTAATGTGAAATGAAGAAATTGTAATGGCAGATAAATAATTAATTTTGATCTTTTCTATGG
TARGET=335,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1144
PRIMER_LEFT_PENALTY=0.080323
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=TCGCTAGTTTCACCAAAACAGA
PRIMER_RIGHT_SEQUENCE=TATTGCTTCCAAGAACCCATCT
PRIMER_LEFT=170,22
PRIMER_RIGHT=372,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=0.1144
PRIMER_LEFT_1_PENALTY=0.080323
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=TCGCTAGTTTCACCAAAACAGA
PRIMER_RIGHT_1_SEQUENCE=CTATTGCTTCCAAGAACCCATC
PRIMER_LEFT_1=170,22
PRIMER_RIGHT_1=373,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.1996
PRIMER_LEFT_2_PENALTY=0.165541
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=CATCGCTAGTTTCACCAAAACA
PRIMER_RIGHT_2_SEQUENCE=TATTGCTTCCAAGAACCCATCT
PRIMER_LEFT_2=168,22
PRIMER_RIGHT_2=372,22
PRIMER_LEFT_2_TM=60.166
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.1996
PRIMER_LEFT_3_PENALTY=0.165541
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=CATCGCTAGTTTCACCAAAACA
PRIMER_RIGHT_3_SEQUENCE=CTATTGCTTCCAAGAACCCATC
PRIMER_LEFT_3=168,22
PRIMER_RIGHT_3=373,22
PRIMER_LEFT_3_TM=60.166
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.2255
PRIMER_LEFT_4_PENALTY=0.073989
PRIMER_RIGHT_4_PENALTY=0.151533
PRIMER_LEFT_4_SEQUENCE=GTTTGTTACCAGTGACGACGAA
PRIMER_RIGHT_4_SEQUENCE=GAATCAACTTCTTCACCCAACC
PRIMER_LEFT_4=309,22
PRIMER_RIGHT_4=601,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=59.848
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_04601 Ps_contig_mira-and-tgicl-ass_franssen_33881
SEQUENCE=CAAAGACAAGTTGTTCTTGGTGATGTGCTAGGGAGAGGGAAAATTAGGGGGTTGGGTTTTTCTAAGGATTGATGTTTTCACAACCATCTTCTCAAGGTTCAGTAGAATCACAAGGTGGAAGTTCTTCATCAGTGTCTGAAATGGATAGCAAACCTTTTCTAGGATCAAGCAGTTGTGGCGAAGCAAGAAGCCCAGCTAGTAATCAGTCCTTACAGGAAAGAAGCAACCAGGACTCCGTCGGTTCATCAGGAGTGCCAAAGCCGAAAGATAATTTGACAAGAACAAGTTCGAGATCCGAGGTAATGGAGAGTGCATCTAAAAAGCCTCATCCCACTCAAAATATTGGGAAGGAAATTGGAACAAACTCAATGGAAGACATGCCTTGTGTATTTACGAAAGGGTGTGGTCCTAATGGAAGAAGAATTGAGGGAATCTTATATAAATATGGCAAAGGGGAAGAAGTGAGGATAATGTGTGTGTGCCATGGAAACTTTCTGTCTCCAGCTGAGTTTGTTAAGCATGCTGGTGGTGAAGATGTTGCTCACCCTCTTAGACATATTGTTGTTAACCCATCTCCTGCCCCCTTTTTGTGATGATTGGAGTCCCTTCAAAGCCGTCTCACATTTTTGAAGGTTCTGATCAGATAAGAGAGACCTCCATTTGACTATGGTTTAAACGTGATAAATATTTATGAAGTCTTTATTTAATACTAAAATTTTAAATTCGTT
TARGET=472,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0930
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.058853
PRIMER_LEFT_SEQUENCE=CATCTAAAAAGCCTCATCCCAC
PRIMER_RIGHT_SEQUENCE=CCAGCATGCTTAACAAACTCAG
PRIMER_LEFT=312,22
PRIMER_RIGHT=525,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.941
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=214
PRIMER_PAIR_PENALTY_1=0.0930
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.058853
PRIMER_LEFT_1_SEQUENCE=AAGCCTCATCCCACTCAAAATA
PRIMER_RIGHT_1_SEQUENCE=CCAGCATGCTTAACAAACTCAG
PRIMER_LEFT_1=320,22
PRIMER_RIGHT_1=525,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.941
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=0.0940
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.059875
PRIMER_LEFT_2_SEQUENCE=AAGCCTCATCCCACTCAAAATA
PRIMER_RIGHT_2_SEQUENCE=CTTTGAAGGGACTCCAATCATC
PRIMER_LEFT_2=320,22
PRIMER_RIGHT_2=612,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.940
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.1156
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.081536
PRIMER_LEFT_3_SEQUENCE=CATCTAAAAAGCCTCATCCCAC
PRIMER_RIGHT_3_SEQUENCE=CAGCATGCTTAACAAACTCAGC
PRIMER_LEFT_3=312,22
PRIMER_RIGHT_3=524,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=60.082
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.1156
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.081536
PRIMER_LEFT_4_SEQUENCE=AAGCCTCATCCCACTCAAAATA
PRIMER_RIGHT_4_SEQUENCE=CAGCATGCTTAACAAACTCAGC
PRIMER_LEFT_4=320,22
PRIMER_RIGHT_4=524,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_04602 Ps_contig_mira-and-tgicl-ass_franssen_33882
SEQUENCE=GGTAAAAGTTACAAGAATCCCCAAACGGTAGGTTNTCCCCACCTCGTAGTATCAGCCTTCCGTGCCTTTTTTTCGGTTAGGCTGGGTTAGGCTGGTCTTTTGTACTGCAGGAGCCTCAGCACCAACACTTTTACTGCTCGGTTTAGCAATCACTTTTGACTTCGGTGGATCGGAGTTCTTAGGTTGTGAATTCATTGTCCCTGATAGTCTTTCACGGAGATCTCGGATGTTCGGAGCAGAACTTTTTCCACTCTGAGTTGCAGGTTGAAGACCTTTCTTTTGAAGCTTATGACGAAGATCTTGACTAGTTACTTTGCGATTAGTGATCTGGGGTTGTTCATCATCATCATATAGATCATGTTCCCACTTGTCATCCTGCCTCGACCTTTTTCCGGTGACTTGGCGTTGCTGCCGAGTAGATACACTGATGTTTCCATCAAGACGTTCCTTGACAGACCTCCTCGCTCCACTTTCCACTCGATCAGCATACATCTTCAACAATTTAGTTAGTTTCCCAAATCAAGAGAAAATAGCCCCAAATCTATGAACCCTAATTTCAAATCAGAGTTGAGATGAAATATAAAGGGAAATGCACTATTAGCGGATGAAAAAGCTGATAGGGTCGCAGAAAATCAAAAACCCTAAAAACCCCAATTTTGTATTATTGATCGTCGACGAGAATGGTAGCGGAGAATAAATAAAAGCGCTTTTCTGAATCGGAGTTGCAGGGAAAAGCTATCAATCGAAGTGTAGATTTTTTGGAGTGAGGTTTTGT
TARGET=338,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1321
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.129818
PRIMER_LEFT_SEQUENCE=GGATCGGAGTTCTTAGGTTGTG
PRIMER_RIGHT_SEQUENCE=AGGAACGTCTTGATGGAAACAT
PRIMER_LEFT=166,22
PRIMER_RIGHT=448,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.1321
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.129818
PRIMER_LEFT_1_SEQUENCE=TGGATCGGAGTTCTTAGGTTGT
PRIMER_RIGHT_1_SEQUENCE=AGGAACGTCTTGATGGAAACAT
PRIMER_LEFT_1=165,22
PRIMER_RIGHT_1=448,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=59.870
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=0.1321
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.129818
PRIMER_LEFT_2_SEQUENCE=GTGGATCGGAGTTCTTAGGTTG
PRIMER_RIGHT_2_SEQUENCE=AGGAACGTCTTGATGGAAACAT
PRIMER_LEFT_2=164,22
PRIMER_RIGHT_2=448,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=59.870
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.1916
PRIMER_LEFT_3_PENALTY=0.088367
PRIMER_RIGHT_3_PENALTY=0.103187
PRIMER_LEFT_3_SEQUENCE=CACTCTGAGTTGCAGGTTGAAG
PRIMER_RIGHT_3_SEQUENCE=GATGTATGCTGATCGAGTGGAA
PRIMER_LEFT_3=248,22
PRIMER_RIGHT_3=492,22
PRIMER_LEFT_3_TM=60.088
PRIMER_RIGHT_3_TM=60.103
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.2179
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.215550
PRIMER_LEFT_4_SEQUENCE=GGATCGGAGTTCTTAGGTTGTG
PRIMER_RIGHT_4_SEQUENCE=GAGGTCTGTCAAGGAACGTCTT
PRIMER_LEFT_4=166,22
PRIMER_RIGHT_4=459,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=59.784
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_04603 Ps_contig_mira-and-tgicl-ass_franssen_33884
SEQUENCE=AAAAGCTTGACCCTTCATCCTTCCTTCCTGCATCAATTTAACTTGAAGACCAGACTTAGCAGCCTCTGTACTTCCAAAGAAGGACCCAAATAAAAAATAGAAATCGTCAGCAATCACATCTTTTGCCAAATTCTTAATATACAACACAGAAGCAGGATTTCCTGCTGTATAGTTCTTAAACATTGGAAGAGATAATATTTCCTCAGGAGGTAACTTTCCTCTCTCTAATTCTTCCGGAGTCAAAAACTTGTTGGGATCTAGAGTCTCTTTTTCTGGCTCTTGTAACTCTGGACTTGTGTCATTATCCTTATGTTCATTCAGGGTTGCTTTGGGAGCAATTTTGATCTTTAAGACGGGGTTCTTTTTTATCATCGGGATTTCTTTCGGGATCAAAGTGGCAGGTTTTACTCCAACAGACCTACATGAGCCACATCTTTATCAATAGCAGGGCCAACAATGGCCCCACCGCCTGGCACGCTTTCGTCCAGAATTTTTTGCTCTTGCTCCATCCTCCTCATCTGAAGATCCCATTTCTGATT
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04604 Ps_contig_mira-and-tgicl-ass_franssen_33895
SEQUENCE=AATAATCTCAAATGTCGTTTCTAGAATTTGTAAATCCTGAGCTCAAAGGGTGATTTCGTAACTTCCCTTATCCCATCTTACTCTCTCCTTTCTTCCTTATCNTCGNGGAATTCCATCAATTCATCTCCCTCCTCCTCCCTACTCGCCATAGCCACAACTCCTATCTCTTTGTGTCTCTTCATAGACACAAAGACACACTAACACTTTCGTTACCATGTCTACGTCTCTTTCTTTTGTTCGTCTCTCTTGTTCCTCGCATTCAATTTCACCCTCTTTTTCTCATCTTCCTCCCCAATTACCCAATTCTCGCGCCTCCCATTTTGTTTTCTGCCGCTACAGACGCAGACACGTTTCTGGTTCCTCCTCCATTAGCCGCGGCATTTTCAAAGTCTGTTCCTTTTCCACTCTCGGCGGAGCTTCTGAATTTGACAGTTTAAGCCAAAGGAGCCATGATGAAGAAGAAGATGATGAACAACAAATTAGGACTAGAGCTAATGACGAGGATGAAGTAGACGATGAAGAAGAAGAAGAAGAAGAAGAATCTATTAGCTCTTATGTTTTTCCTGAAAGATGGGATGTCTTAGGTCTTGGACAAGCCATGGTAGACTTTTCTGGCACAATTGATGATGAGTTCTTGAAGAATTTAGGATTAGAAAAGGGAACAAGGAAACTTGTAAATCATGAGGAAAGAGGTAGAGTTTTGCAGGCCATGGATGGTTGCAGTTATAAAGCCGCCGCTGGTGGATCTCTTTCTAATACCTTAGTTGCCCTGGCAAGGCTTGGAGGCCGCTCCCTCCGAGATCCTGCTATAAATGTGGGAATGGCTGGGAGTGTCGCGAGTGATCTATTGGGTGGTTTCTACAGGGAAAAATTACGCCGAGCAAATGTGCAATTTCTGTCTGTGCCTATCAAGGATGCGACAACTGGAACAGTTATAGTTCTTACAACTCCAGATGCTCAACGCACAATGCTTGCATATCAGGGCACATCTTCAACTGTTAACTTTGACACTTCCTTGGCAAGTGCAGTTTCCAAGACCAACATACTTGTTGTTGAAGGGTATCTATTCGAACTTCCCGATACTATTAAAACAATAACAAAAGCATGTATGGAAGCTAGGAGTAATGGTGCTCTGGTTGCAGTAACAGCTTCAGATGTCTCCTGCATTGAGAGACATTTTGATCATTTCTGGGAGATCATAGGCAATTACGCAGATTTAATATTCGCTAACGTTGATGAGGCGAGAGCTCTTTGTAATTTTGATGCAAAGGAGAGTACTGTTTCTGTTACAAGATATCTAAGTCAGTTTGTCCCTCTAGTATCCGTTACAGACGGTATCAGAGGCTCTTACATAGGCGTAAAAGGTGAAGCTGTATACATCCCTCCCTCTCCATGTGTTCCAGTAGATACATGTGGTGCTGGTGATGCATACGCATCTGGCATTCTGTACGGTGTTTTACGAGGCATGTCGGATTTGAGAAGTATCGGTACAATAGCATCTAAGGTTGCGTCCACTGTTGTTGCGCAGCAGGGAACGAGGCTACGGATTTCAGATGCAGTTAAGTTGGCTGAATCTTTTGCATTTCAACTTGATACATCGACTGTTCGATCTGATGTTGGTACTGATCATATTTCCAGTGTTTAATTAGATTGTAAACTTACATTTAAGTTCCAAAATTCTTTCATAAGATGAAGGAAGGAATGTAGTCCTTTGATTTATTTCAAAAGTCATGTAAATACTTTCATTGTAAAAAGATAGCTATAGCTATGATGACAATTAAATTATTTAGTATTTCTGACCATTCTTCAATCT
TARGET=518,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1516
PRIMER_LEFT_PENALTY=0.151533
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=AGGAGCCATGATGAAGAAGAAG
PRIMER_RIGHT_SEQUENCE=AACTCATCATCAATTGTGCCAG
PRIMER_LEFT=442,22
PRIMER_RIGHT=632,22
PRIMER_LEFT_TM=59.848
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.2078
PRIMER_LEFT_1_PENALTY=0.207756
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=AAGGAGCCATGATGAAGAAGAA
PRIMER_RIGHT_1_SEQUENCE=AACTCATCATCAATTGTGCCAG
PRIMER_LEFT_1=441,22
PRIMER_RIGHT_1=632,22
PRIMER_LEFT_1_TM=60.208
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.2078
PRIMER_LEFT_2_PENALTY=0.207756
PRIMER_RIGHT_2_PENALTY=0.000037
PRIMER_LEFT_2_SEQUENCE=AAAGGAGCCATGATGAAGAAGA
PRIMER_RIGHT_2_SEQUENCE=AACTCATCATCAATTGTGCCAG
PRIMER_LEFT_2=440,22
PRIMER_RIGHT_2=632,22
PRIMER_LEFT_2_TM=60.208
PRIMER_RIGHT_2_TM=60.000
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=193
PRIMER_PAIR_PENALTY_3=0.2811
PRIMER_LEFT_3_PENALTY=0.281044
PRIMER_RIGHT_3_PENALTY=0.000037
PRIMER_LEFT_3_SEQUENCE=GTCTGTTCCTTTTCCACTCTCG
PRIMER_RIGHT_3_SEQUENCE=AACTCATCATCAATTGTGCCAG
PRIMER_LEFT_3=388,22
PRIMER_RIGHT_3=632,22
PRIMER_LEFT_3_TM=60.281
PRIMER_RIGHT_3_TM=60.000
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.2818
PRIMER_LEFT_4_PENALTY=0.151533
PRIMER_RIGHT_4_PENALTY=0.130244
PRIMER_LEFT_4_SEQUENCE=AGGAGCCATGATGAAGAAGAAG
PRIMER_RIGHT_4_SEQUENCE=GGCTTTATAACTGCAACCATCC
PRIMER_LEFT_4=442,22
PRIMER_RIGHT_4=732,22
PRIMER_LEFT_4_TM=59.848
PRIMER_RIGHT_4_TM=59.870
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_04605 Ps_contig_mira-and-tgicl-ass_franssen_33900
SEQUENCE=TTAAACTAATAGATTCAAACTTGTTCATATTCAGTTTTTNGAAAATAGATTCACTTCAGTTTGAACATGTATCAACCAGTTGCAATTGACAGTAGACAAGAAATGTAATAATGTATTAAAGTGATTATTGACTCTACAAGTTCATTCCACTAAAGCTTGAGCAGAAGTATAACGGTAATAATTTGACATGTTAACATCCGGCATCTCCGAAATAGATCTGCAAAACTCGGAGATCCACTAATGCCACAATTCCAATACCGAAAGTACAAAACAAAAAAGGAAAGGGGCATGTAAGAAATTCGTACATGAAGTAAAAATTCTCAAGGCGTGATAATAAATCCCAGCACTGACTTTCCAGCTGAGAGTCTGCTTGCGTTATTCAAAGAATCAGGAATTGAAACAGAAAATTTCCTATATCGAAATAAATTCAAGATCGAAGAGCACTTCAACATCCATCAGATTTGACTCGAGTCCCTCCCAAAAATAAAGCGTCTCATCCAGTCTGACACCACTAATGCCCTTGTGCGCCAGCTGACTTGCTTGCTTGCATAGACAGAATACCACAGCCATTGACTGCTGTGTCCAATTGAAACCCAATCTCCAGGTAGCTGTGCAGCTGTCTGTTCTCCTCCCAGAGGAGCAAATTGACCTAAATGCTTGTACCTGAAGGGTTTGAACCGATGACGACCTTCTTCCCTGAACCTAATAGGACCCTCTGGGTTTTTTTCACACTCTTCCATCCGGTTAAAACACTTGGCCAGGTAGGCTCCTTGCTGAGATGCAACCTGAGCTGTAGCAGGAAGAAATTTCATCTGCGAGTCCACATTGAAAAGTGCAGTTTTGAGTTCTTCGATATTAATTTCAATAGATTCTTTTTCAACATCTCCTTTGGATTCTTCCAAAAGATCAGCAATGCCATGCATCTGTTTATTCTTTAGGAATAGCTCCACTTGAGGGTATCTTTCAAAGATGTCATCCATTACCGCCTGAAATTCTTTGAGCGTAATTGTTCCTGAGTTGTCTGCATCAGTCTTCTTAAATATTGCCACAATATCTTCCATGACTTTTCGCTGGTTTATTGTAGCACAATCACCAAGCGCATACACATTATTGGTTCCCTCAACTCTCAACCATTCATCAGTAGCTACGGCACGTCTGTTAATCTGACCAATTTGTGTCATAAAATCTTTTATGAATGGACGAGTGCCAATACCAGTTGACCAGACAGCCATTCCATATGGAATTGAAGTCATTTCTCCACTTTTCATTTCTTTAGTGGAAATTTCTCTGTCAGATACCTTCACAACCATGGATCCTGTTTTCACATCAATACCATCTCTGCGGAACTTATCTTCAGCAAAAGCTGTTATTCTTTTGTCAAACATGCTCAAGATATGACCTCCAGCCTCCAGAAGGGTAATTTTAAATAAATCTTTTACCCCGGGATATAATTTGACCAAATCCTCACTGACAAAATCATGAAGTGCGGCTGCAAACTCCACTCCAGTTGGACCACCTCCAACAATAGCAAAATGAAGAATTCTCTTTTTTTCTTTCATCACTTACACTAGGCAAACTTGCTCTCTCAAAGCAGTCAATTACAGTTCTTCTAATCCTCTGCGCATCTTCAACTTCCTTCAAGAAATGACAATTCTCCGTGACTCCAGGAGTATTAAATGTGTTGACATTAGCCCCCACAGCTATGATTAAGTAGTCATAGTCCACAACGAATTCATTCTTTTTC
TARGET=536,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1438
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.128591
PRIMER_LEFT_SEQUENCE=TAAAGCGTCTCATCCAGTCTGA
PRIMER_RIGHT_SEQUENCE=GTTTTAACCGGATGGAAGAGTG
PRIMER_LEFT=484,22
PRIMER_RIGHT=750,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.871
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.1438
PRIMER_LEFT_1_PENALTY=0.015253
PRIMER_RIGHT_1_PENALTY=0.128591
PRIMER_LEFT_1_SEQUENCE=TAAAGCGTCTCATCCAGTCTGA
PRIMER_RIGHT_1_SEQUENCE=TTTTAACCGGATGGAAGAGTGT
PRIMER_LEFT_1=484,22
PRIMER_RIGHT_1=749,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=59.871
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.1587
PRIMER_LEFT_2_PENALTY=0.030114
PRIMER_RIGHT_2_PENALTY=0.128591
PRIMER_LEFT_2_SEQUENCE=CCCAAAAATAAAGCGTCTCATC
PRIMER_RIGHT_2_SEQUENCE=GTTTTAACCGGATGGAAGAGTG
PRIMER_LEFT_2=476,22
PRIMER_RIGHT_2=750,22
PRIMER_LEFT_2_TM=59.970
PRIMER_RIGHT_2_TM=59.871
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.1587
PRIMER_LEFT_3_PENALTY=0.030114
PRIMER_RIGHT_3_PENALTY=0.128591
PRIMER_LEFT_3_SEQUENCE=CCCAAAAATAAAGCGTCTCATC
PRIMER_RIGHT_3_SEQUENCE=TTTTAACCGGATGGAAGAGTGT
PRIMER_LEFT_3=476,22
PRIMER_RIGHT_3=749,22
PRIMER_LEFT_3_TM=59.970
PRIMER_RIGHT_3_TM=59.871
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.1587
PRIMER_LEFT_4_PENALTY=0.030114
PRIMER_RIGHT_4_PENALTY=0.128591
PRIMER_LEFT_4_SEQUENCE=CCAAAAATAAAGCGTCTCATCC
PRIMER_RIGHT_4_SEQUENCE=GTTTTAACCGGATGGAAGAGTG
PRIMER_LEFT_4=477,22
PRIMER_RIGHT_4=750,22
PRIMER_LEFT_4_TM=59.970
PRIMER_RIGHT_4_TM=59.871
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_04606 Ps_contig_mira-and-tgicl-ass_franssen_33909
SEQUENCE=ACATCGATCATAGATAGATAGATACACACTCCTNACGTCACTCCCACTTGTTCCACAAACAAACACACCACATTACACATTCCCACATTCTAACCATTACATAATCTTCACCCTAAACTCATCTCTCTGTTTCTCAACAGAACCAGAGAATGCAGCACTTCACCCTCGTTACGATCTTTCTCGTACTTTCCCTCACCCTTCTCACTCCCTCTGATGGAGGTTCCATAGGAATAAACTACGGTCGCATAGCCAACAACCTCCCCTCCGCCATGAAAGTAGTCCATCTCCTCAAATCACAAGGTCTGGACCGTGTCAAAGTCTACGACACCGACCCTTCAGTTCTCCGAGCATTATCCGGATCCAAAATCAAAGTAACCGTTGATCTCCCAAACCAACAACTCTTCGCAGCCGCAAAAGCACCTTCCTTTGCTCTGTCATGGGTCGAACGAAACGTCGTAGCTTACTACCCTCACACCCAAATCGAAGCCATTGCAGTTGGTAACGAAGTCTTCGTGGATCCCAGCAACACCACAAAGTTCCTCGTACCCGCCATGAAAAACATCTACAGAGCACTCCAAAAACATAACCTCCACTCCGACATTAAAGTTTCCTCCCCCATCGCACTTTCCGCACTTGGTAACTCTTACCCTTCCTCATCAGGGTCATTCCGACCCGAGTTAATCCAACCCGTTTTTAAACCCATGCTAGATTTTATCCGCGAAACCGGTTCTTACCTTATGGTAAACGTTTACCCTTTTTTCGCTTACGAGTCAAACGCTGACGTCATCTCTTTAGATTATGCTCTATTTAGACAAAACCCGGGTCAGGTGGATCCGGGTAACGGGTTAAGATATACAAACCTCTTCGACGCTCAAATCGACGCCGTTTTCGCTGCACTCAATAGTTTGAAATACGACGATGTGAACATTGTCGTTTCAGAGACAGGCTGGCCTTCGAAAGGTGATGGCAATGAGGTGGGTGCGAGTGTAGAGAATGCAGCTGCTTACAACGGGAATCTCGTCCGTAAGATCTTGACTGGAAGTGGGACCCCTTTGAGACCTAAAGCAGATCTAACCGTTTATTTATTCGCACTTTTCAATGAAAATCAGAAACCTGGCCCCACTTCAGAGAGAAATTTCGGTTTGTTTTACCCTAATGAGAAGAAGGTTTACGACGTTCCATTAACCGTGGAGGCGCTTAAAAATTACCACGACAATCCGTCACCGGTTGCCGGTGGTGCAAACCAGCCTACTCCGTCGGAGGGAGGTGGTAATGGCGGTGTTTCGAAGAGTACTACCGGGAACACGTGGTGCGTTGCGAATCCTTACGCGGATAAAAATAAGCTACAAGCCGCTTTAGATTTTGCTTGTGGTGAAGGAGGTGCTGATTGCCGTTCGATTCAGCCGAACTCCACGTGTTACAATCCTAACACATTGGTTTCGCACGCTTCGTTTGCGTTTAACAGTTATTATCAAAAACAAGCACGTGCTGGCGGGAGTTGCTATTTCGGTGGTACGTCGTACGTGGTCACGCAAGAGCCTAAATATGGTGACTGTGAATATCCCACTGGATATTAAAAGCTGATTTTGCACTGTGTTGAAGGGTGAAGATGAAGAGATTTGGTTGATGATTAGTTAAAATAATGTTTAGTACTTGACTTGTCATTAAGTTGGTGGGAGTTGGTTAGCTAAATTTACTTTTAGAGACTTTAATTAAATTGTATCTAAGTTTTTATTATTTTGGAGTAGTAGTTCATTTTCATTTGCTTAGACTAGAAGTAGTAATCAGTTTTCATTTTGCTAATACATTATAGTTTCTTTAATTTTAATCTAAGTTTGAGTAAATATAATTTTCTTTGCTACCTTTGAA
TARGET=10,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04607 Ps_contig_mira-and-tgicl-ass_franssen_33928
SEQUENCE=AACATAATGTCAACATAATTGCAATAAGAAAGACAAATCAAGTCTTCATTAACATAATAGCGAATATGGCAAATTGAGATATAATCTTAAACTTTGCCGTAATGAAGAGAAAAAAAACAATCAACAGTAGTTAGTTGCAAGCATGCAACTCTCCAAACAAAAGAAACATGAAACTCAATAACAAACATTTAAATTACAACATCAAAATAAGAGAGAATCCAAATATGAAAAACATCTTCATTCATCATACCTATGTTGAAGATGTAGTAGCAAGCCTTCCACGTCCAATACCACCACTATGCTGATGATCACACTCTCTTCTTTTCATCAAACTCTCCTCTTTCGAACTCAATCTCGAACTCCAACAATCCGGTGAATCGCCAGAAGAACAATTGTTCCAATGTTGTTGTTGTTGTTGTTGACCACTATTCAAACTGAAGCTATTACTTCCAGAAGACTCTTGATCCTCACAAAGTGAAAACGAACCACCTCTTTTAACAGTAACATACTTATGCAAACTTGGTTCCATCACATCATCACTCACAACAACTTCAGATTCTTCTACAAATGATGATTCAGGAACAGCATTGTACTCGTTCCTAGAGAAAGTTGAACTTGTTGTGGAGTTGAGATCAGCAACAGCCTCAAGGATTGAGCAAGCTTTGTTGAGACAAGCTGGAAGACACACAGCAGGCTGTGCTGTGGTCTTCTGATGGAAGTTATGAATGTCTTCTAGGAGTAATGAAGTGTAGGAAGATGGTGGTGGATTGGTTAGGGTTTCGGGATTGATGTTGTCTAAGTCTCTTGATCTTCTAGATGATCTGCTTCTTGTAATCCCTGATGGCATCACCACATTCTTCACAATAGCATTATCAGACATTACCTTGATT
TARGET=402,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2011
PRIMER_LEFT_PENALTY=0.168114
PRIMER_RIGHT_PENALTY=0.032945
PRIMER_LEFT_SEQUENCE=CACGTCCAATACCACCACTATG
PRIMER_RIGHT_SEQUENCE=TTGTGAGTGATGATGTGATGGA
PRIMER_LEFT=279,22
PRIMER_RIGHT=545,22
PRIMER_LEFT_TM=60.168
PRIMER_RIGHT_TM=59.967
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.2250
PRIMER_LEFT_1_PENALTY=0.168114
PRIMER_RIGHT_1_PENALTY=0.056850
PRIMER_LEFT_1_SEQUENCE=CACGTCCAATACCACCACTATG
PRIMER_RIGHT_1_SEQUENCE=AGGTGGTTCGTTTTCACTTTGT
PRIMER_LEFT_1=279,22
PRIMER_RIGHT_1=490,22
PRIMER_LEFT_1_TM=60.168
PRIMER_RIGHT_1_TM=59.943
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.3146
PRIMER_LEFT_2_PENALTY=0.168114
PRIMER_RIGHT_2_PENALTY=0.146525
PRIMER_LEFT_2_SEQUENCE=CACGTCCAATACCACCACTATG
PRIMER_RIGHT_2_SEQUENCE=CGTTTTCACTTTGTGAGGATCA
PRIMER_LEFT_2=279,22
PRIMER_RIGHT_2=482,22
PRIMER_LEFT_2_TM=60.168
PRIMER_RIGHT_2_TM=60.147
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=204
PRIMER_PAIR_PENALTY_3=0.3501
PRIMER_LEFT_3_PENALTY=0.168114
PRIMER_RIGHT_3_PENALTY=0.182028
PRIMER_LEFT_3_SEQUENCE=CACGTCCAATACCACCACTATG
PRIMER_RIGHT_3_SEQUENCE=TGGTTCGTTTTCACTTTGTGAG
PRIMER_LEFT_3=279,22
PRIMER_RIGHT_3=487,22
PRIMER_LEFT_3_TM=60.168
PRIMER_RIGHT_3_TM=60.182
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.3557
PRIMER_LEFT_4_PENALTY=0.322785
PRIMER_RIGHT_4_PENALTY=0.032945
PRIMER_LEFT_4_SEQUENCE=GTAGTAGCAAGCCTTCCACGTC
PRIMER_RIGHT_4_SEQUENCE=TTGTGAGTGATGATGTGATGGA
PRIMER_LEFT_4=263,22
PRIMER_RIGHT_4=545,22
PRIMER_LEFT_4_TM=60.323
PRIMER_RIGHT_4_TM=59.967
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_04608 Ps_contig_mira-and-tgicl-ass_franssen_33933
SEQUENCE=AAACGAAACGGCGAGGAGACGGTCCGAAGGGACGAATGACGATTACGAAAATCTCTCCACAGGATTGAAAATTGAAATCCCTCTTTCTCTGTTTCTCATCTCATCTCATACCAATCAATCAATCATTCAATAAAATGGCAAATGTTTTGAAGGGTGGTAGTGTTGTTATGCTCTCATTTTTCTCTTCACGAACCACCACACTCTCCCGAACATCAACAACAACAACAACCCACTGCTCATTTCTTCCCAAGTTCCCTTTTTTCTCACTCTCATCTTCCTCACGCCCCAAAACCCCATCTGGGTTTCCTTCCTATTGCACTCTTGCTTCCGACCCAGTCCCGGAAGCCATAGCTGCTGAAACCAATAGCAACGGTGAAAATGACAAGAGTATTAAAGATGCTGCTGGGTTGCTTGACATAAGGGTGGGTCGCATTATCAGGGCATGGAAACACGATGAAGCCGAAACTCTTTACGTTGAAGAAGTTGATATTGGTGAGCCTGAACCTAGAATCATTTGCAGTGGCCTTGTCAATTACATCCCTCTTGAACACCTTCAGGGGAAAAAAGTTATTGTTCTTTCGAATCTGAAGCCAAGGAATATGCGTGGTGTCAAGTCATGTGGAATGTTGATGGCTGCTTCTGATTCAAAGCATGAGAATGTTGAACTTCTGTTCCCTCCCGAGGAAGCAAATCCTGGCGAAAGAATATGGTTTGGATCTGAAGATGAAAAGGACAATCAACCTGAAGCTGCCAAACCGAATCAGATACAAAAGAAAAAGATATGGGAATTGGTGCAGCCTCATCTTAAGACAGATGTTTCTTGTACGGCGATGCTAGGTGAGCATGTAATGAGTACATCTGTAGGCACAGTGGCATGCCAGTCATTGCAGAATGCAAATATCTCCTGACCAACACTCACTCTCTCAATTTACACATCGGAATAGGCAATGTAATTCTTCTTTTTTTGGCTCGTGTATAAACATTTGTTCTTTATATTTTTATGATTTTAAATTGTTTTTCAGAAGTTTCACTTGGAAATAAAGGTTTCAGCTTAGCAATANTATCTTGTCATGGTTGTCAATAATTTTACAATT
TARGET=94,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8151
PRIMER_LEFT_PENALTY=0.775205
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=CGATTACGAAAATCTCTCCACA
PRIMER_RIGHT_SEQUENCE=AAGAAATGAGCAGTGGGTTGTT
PRIMER_LEFT=39,22
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=59.225
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.8622
PRIMER_LEFT_1_PENALTY=0.775205
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=CGATTACGAAAATCTCTCCACA
PRIMER_RIGHT_1_SEQUENCE=AAGCAAGAGTGCAATAGGAAGG
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=326,22
PRIMER_LEFT_1_TM=59.225
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.9457
PRIMER_LEFT_2_PENALTY=0.775205
PRIMER_RIGHT_2_PENALTY=0.170538
PRIMER_LEFT_2_SEQUENCE=CGATTACGAAAATCTCTCCACA
PRIMER_RIGHT_2_SEQUENCE=CAATAGGAAGGAAACCCAGATG
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=315,22
PRIMER_LEFT_2_TM=59.225
PRIMER_RIGHT_2_TM=59.829
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=1.0165
PRIMER_LEFT_3_PENALTY=0.976652
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=CGAAAATCTCTCCACAGGATTG
PRIMER_RIGHT_3_SEQUENCE=AAGAAATGAGCAGTGGGTTGTT
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=244,22
PRIMER_LEFT_3_TM=60.977
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=1.0167
PRIMER_LEFT_4_PENALTY=0.775205
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=CGATTACGAAAATCTCTCCACA
PRIMER_RIGHT_4_SEQUENCE=GAACTTGGGAAGAAATGAGCAG
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=253,22
PRIMER_LEFT_4_TM=59.225
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_04609 Ps_contig_mira-and-tgicl-ass_franssen_33933
SEQUENCE=AAACGAAACGGCGAGGAGACGGTCCGAAGGGACGAATGACGATTACGAAAATCTCTCCACAGGATTGAAAATTGAAATCCCTCTTTCTCTGTTTCTCATCTCATCTCATACCAATCAATCAATCATTCAATAAAATGGCAAATGTTTTGAAGGGTGGTAGTGTTGTTATGCTCTCATTTTTCTCTTCACGAACCACCACACTCTCCCGAACATCAACAACAACAACAACCCACTGCTCATTTCTTCCCAAGTTCCCTTTTTTCTCACTCTCATCTTCCTCACGCCCCAAAACCCCATCTGGGTTTCCTTCCTATTGCACTCTTGCTTCCGACCCAGTCCCGGAAGCCATAGCTGCTGAAACCAATAGCAACGGTGAAAATGACAAGAGTATTAAAGATGCTGCTGGGTTGCTTGACATAAGGGTGGGTCGCATTATCAGGGCATGGAAACACGATGAAGCCGAAACTCTTTACGTTGAAGAAGTTGATATTGGTGAGCCTGAACCTAGAATCATTTGCAGTGGCCTTGTCAATTACATCCCTCTTGAACACCTTCAGGGGAAAAAAGTTATTGTTCTTTCGAATCTGAAGCCAAGGAATATGCGTGGTGTCAAGTCATGTGGAATGTTGATGGCTGCTTCTGATTCAAAGCATGAGAATGTTGAACTTCTGTTCCCTCCCGAGGAAGCAAATCCTGGCGAAAGAATATGGTTTGGATCTGAAGATGAAAAGGACAATCAACCTGAAGCTGCCAAACCGAATCAGATACAAAAGAAAAAGATATGGGAATTGGTGCAGCCTCATCTTAAGACAGATGTTTCTTGTACGGCGATGCTAGGTGAGCATGTAATGAGTACATCTGTAGGCACAGTGGCATGCCAGTCATTGCAGAATGCAAATATCTCCTGACCAACACTCACTCTCTCAATTTACACATCGGAATAGGCAATGTAATTCTTCTTTTTTTGGCTCGTGTATAAACATTTGTTCTTTATATTTTTATGATTTTAAATTGTTTTTCAGAAGTTTCACTTGGAAATAAAGGTTTCAGCTTAGCAATANTATCTTGTCATGGTTGTCAATAATTTTACAATT
TARGET=112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8151
PRIMER_LEFT_PENALTY=0.775205
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=CGATTACGAAAATCTCTCCACA
PRIMER_RIGHT_SEQUENCE=AAGAAATGAGCAGTGGGTTGTT
PRIMER_LEFT=39,22
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=59.225
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.8622
PRIMER_LEFT_1_PENALTY=0.775205
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=CGATTACGAAAATCTCTCCACA
PRIMER_RIGHT_1_SEQUENCE=AAGCAAGAGTGCAATAGGAAGG
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=326,22
PRIMER_LEFT_1_TM=59.225
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.9457
PRIMER_LEFT_2_PENALTY=0.775205
PRIMER_RIGHT_2_PENALTY=0.170538
PRIMER_LEFT_2_SEQUENCE=CGATTACGAAAATCTCTCCACA
PRIMER_RIGHT_2_SEQUENCE=CAATAGGAAGGAAACCCAGATG
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=315,22
PRIMER_LEFT_2_TM=59.225
PRIMER_RIGHT_2_TM=59.829
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=1.0165
PRIMER_LEFT_3_PENALTY=0.976652
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=CGAAAATCTCTCCACAGGATTG
PRIMER_RIGHT_3_SEQUENCE=AAGAAATGAGCAGTGGGTTGTT
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=244,22
PRIMER_LEFT_3_TM=60.977
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=1.0167
PRIMER_LEFT_4_PENALTY=0.775205
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=CGATTACGAAAATCTCTCCACA
PRIMER_RIGHT_4_SEQUENCE=GAACTTGGGAAGAAATGAGCAG
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=253,22
PRIMER_LEFT_4_TM=59.225
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_04610 Ps_contig_mira-and-tgicl-ass_franssen_33933
SEQUENCE=AAACGAAACGGCGAGGAGACGGTCCGAAGGGACGAATGACGATTACGAAAATCTCTCCACAGGATTGAAAATTGAAATCCCTCTTTCTCTGTTTCTCATCTCATCTCATACCAATCAATCAATCATTCAATAAAATGGCAAATGTTTTGAAGGGTGGTAGTGTTGTTATGCTCTCATTTTTCTCTTCACGAACCACCACACTCTCCCGAACATCAACAACAACAACAACCCACTGCTCATTTCTTCCCAAGTTCCCTTTTTTCTCACTCTCATCTTCCTCACGCCCCAAAACCCCATCTGGGTTTCCTTCCTATTGCACTCTTGCTTCCGACCCAGTCCCGGAAGCCATAGCTGCTGAAACCAATAGCAACGGTGAAAATGACAAGAGTATTAAAGATGCTGCTGGGTTGCTTGACATAAGGGTGGGTCGCATTATCAGGGCATGGAAACACGATGAAGCCGAAACTCTTTACGTTGAAGAAGTTGATATTGGTGAGCCTGAACCTAGAATCATTTGCAGTGGCCTTGTCAATTACATCCCTCTTGAACACCTTCAGGGGAAAAAAGTTATTGTTCTTTCGAATCTGAAGCCAAGGAATATGCGTGGTGTCAAGTCATGTGGAATGTTGATGGCTGCTTCTGATTCAAAGCATGAGAATGTTGAACTTCTGTTCCCTCCCGAGGAAGCAAATCCTGGCGAAAGAATATGGTTTGGATCTGAAGATGAAAAGGACAATCAACCTGAAGCTGCCAAACCGAATCAGATACAAAAGAAAAAGATATGGGAATTGGTGCAGCCTCATCTTAAGACAGATGTTTCTTGTACGGCGATGCTAGGTGAGCATGTAATGAGTACATCTGTAGGCACAGTGGCATGCCAGTCATTGCAGAATGCAAATATCTCCTGACCAACACTCACTCTCTCAATTTACACATCGGAATAGGCAATGTAATTCTTCTTTTTTTGGCTCGTGTATAAACATTTGTTCTTTATATTTTTATGATTTTAAATTGTTTTTCAGAAGTTTCACTTGGAAATAAAGGTTTCAGCTTAGCAATANTATCTTGTCATGGTTGTCAATAATTTTACAATT
TARGET=214,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4091
PRIMER_LEFT_PENALTY=0.368190
PRIMER_RIGHT_PENALTY=0.040897
PRIMER_LEFT_SEQUENCE=TGCTCTCATTTTTCTCTTCACG
PRIMER_RIGHT_SEQUENCE=TTGCTATTGGTTTCAGCAGCTA
PRIMER_LEFT=168,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=59.632
PRIMER_RIGHT_TM=60.041
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.4552
PRIMER_LEFT_1_PENALTY=0.368190
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=TGCTCTCATTTTTCTCTTCACG
PRIMER_RIGHT_1_SEQUENCE=AAGCAAGAGTGCAATAGGAAGG
PRIMER_LEFT_1=168,22
PRIMER_RIGHT_1=326,22
PRIMER_LEFT_1_TM=59.632
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.5337
PRIMER_LEFT_2_PENALTY=0.368190
PRIMER_RIGHT_2_PENALTY=0.165541
PRIMER_LEFT_2_SEQUENCE=TGCTCTCATTTTTCTCTTCACG
PRIMER_RIGHT_2_SEQUENCE=CTTGTCATTTTCACCGTTGCTA
PRIMER_LEFT_2=168,22
PRIMER_RIGHT_2=385,22
PRIMER_LEFT_2_TM=59.632
PRIMER_RIGHT_2_TM=60.166
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=0.5367
PRIMER_LEFT_3_PENALTY=0.495821
PRIMER_RIGHT_3_PENALTY=0.040897
PRIMER_LEFT_3_SEQUENCE=ATTTTTCTCTTCACGAACCACC
PRIMER_RIGHT_3_SEQUENCE=TTGCTATTGGTTTCAGCAGCTA
PRIMER_LEFT_3=175,22
PRIMER_RIGHT_3=369,22
PRIMER_LEFT_3_TM=59.504
PRIMER_RIGHT_3_TM=60.041
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=0.5387
PRIMER_LEFT_4_PENALTY=0.368190
PRIMER_RIGHT_4_PENALTY=0.170538
PRIMER_LEFT_4_SEQUENCE=TGCTCTCATTTTTCTCTTCACG
PRIMER_RIGHT_4_SEQUENCE=CAATAGGAAGGAAACCCAGATG
PRIMER_LEFT_4=168,22
PRIMER_RIGHT_4=315,22
PRIMER_LEFT_4_TM=59.632
PRIMER_RIGHT_4_TM=59.829
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_04611 Ps_contig_mira-and-tgicl-ass_franssen_33936
SEQUENCE=CCTTATTGAAGCCAGACAAATAGAGTCTNTAAAACTGAAGGGTTTTACATCATTTCATAGACAAATGAGTAAATAAAATGATATATAGTACAAGTTTAAAAATCATATAAACTATATAATTCTAGACTCGTCCCTATTCTATTTGCATGATATGGTGCCATATCATGGAACGTGCGAAAGAAACCTCGACTACCATCAAACAATTTGCTCAACAATCCAATAATGTCACTTTTCTTTTTCATTGATTGATAAAAAAAAATAGTAGCAAACCATACATTAATCACAAAGCAAACGTATAACAGGAAAAACATCCATATGGTGCCTATGAAGAAGCATGATGTCGCACAATGCATATACCATTCAGAATTTAGGGATGGGACCAAATTTAGTCTGGTTAAGAGTCCTCGTCCAATTCGTCAGAGTCAGAATCACTTGCATTATAACCAGGACGCTTATCGCCAACTTTCCAAACTGAAGATCTAACCCTTGATGCTTTCGTTAGCCAAAATTTACTCTTTTGAGCATCTTTAGGAACACCATAGCCACTATAATACATCTGACCAACCAAAACCTGCATATTAACATCACCACCTTTAGCTTCCCTTAGAGTATCCTTAAACCAACGCTTCACACAATCAGCTACCACCCCTGACAAAGGAACACGTTCGTTGCTATCTTTCCCTATGCTGCTCTCCATGTATCTCTTCCAATTTCAACTTCAGCACGAGGGTTTTCTTCAAAAACTGGTTTTCCAATACCCTAAAAAAAACCCAAAAATTGTGAAATTGTTAACTTTCTAGGGTTTTGGAAGCTGTGAGTGATGCTATATAGAGAACATGGAAGGATTTCAATGGAGAAATTATAAAGGGTTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCGCTAGTACGGAAAAGGAAAAAAGGACGTA
TARGET=872,34
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2986
PRIMER_LEFT_PENALTY=0.095523
PRIMER_RIGHT_PENALTY=0.203106
PRIMER_LEFT_SEQUENCE=TGGAAGCTGTGAGTGATGCTAT
PRIMER_RIGHT_SEQUENCE=TTTTTCCTTTTCCGTACTAGCG
PRIMER_LEFT=805,22
PRIMER_RIGHT=927,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=59.797
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=9.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=0.3385
PRIMER_LEFT_1_PENALTY=0.135429
PRIMER_RIGHT_1_PENALTY=0.203106
PRIMER_LEFT_1_SEQUENCE=CGAGGGTTTTCTTCAAAAACTG
PRIMER_RIGHT_1_SEQUENCE=TTTTTCCTTTTCCGTACTAGCG
PRIMER_LEFT_1=724,22
PRIMER_RIGHT_1=927,22
PRIMER_LEFT_1_TM=60.135
PRIMER_RIGHT_1_TM=59.797
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.3668
PRIMER_LEFT_2_PENALTY=0.163658
PRIMER_RIGHT_2_PENALTY=0.203106
PRIMER_LEFT_2_SEQUENCE=TGCTCTCCATGTATCTCTTCCA
PRIMER_RIGHT_2_SEQUENCE=TTTTTCCTTTTCCGTACTAGCG
PRIMER_LEFT_2=687,22
PRIMER_RIGHT_2=927,22
PRIMER_LEFT_2_TM=59.836
PRIMER_RIGHT_2_TM=59.797
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=0.3677
PRIMER_LEFT_3_PENALTY=0.164560
PRIMER_RIGHT_3_PENALTY=0.203106
PRIMER_LEFT_3_SEQUENCE=TAGGGTTTTGGAAGCTGTGAGT
PRIMER_RIGHT_3_SEQUENCE=TTTTTCCTTTTCCGTACTAGCG
PRIMER_LEFT_3=797,22
PRIMER_RIGHT_3=927,22
PRIMER_LEFT_3_TM=60.165
PRIMER_RIGHT_3_TM=59.797
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=0.3954
PRIMER_LEFT_4_PENALTY=0.192310
PRIMER_RIGHT_4_PENALTY=0.203106
PRIMER_LEFT_4_SEQUENCE=AGGAACACGTTCGTTGCTATCT
PRIMER_RIGHT_4_SEQUENCE=TTTTTCCTTTTCCGTACTAGCG
PRIMER_LEFT_4=655,22
PRIMER_RIGHT_4=927,22
PRIMER_LEFT_4_TM=60.192
PRIMER_RIGHT_4_TM=59.797
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=9.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_04612 Ps_contig_mira-and-tgicl-ass_franssen_33938
SEQUENCE=AAATTAGAAAAAACCATACCTATNTTGGGTGGTAGTGGAAACATCCACTTTTTCTTCTCATTCTAATTCCTTTCTCTTCTCTTCACTCCACTCCACTACAAAGCTTCTTCATTTCTCAACACTTTCCTCTCATCTCACCATTCACAATGACCGAACCAACTTCCAAACCTGCAATTAACGGTGCCGCCACAACTAACGGAAATGGAAATGGAAATGGAAATCCACTTCCCGTTAAAGCTCAGCTTTACAATCCAAACCGTCAAGTTTACCGTCCTCAATCACAATACAACCGTCGCCGTCGATCTAACCGTAGCCTCTGCTGTTGCTGCTGTTTCTGGACAATCCTCACAGTTCTCGCCGCCGCACTACTCGTTGCTATTGTCGGTGCTGCACTCTACGTACTCTACCATCCTCACCAACCAGAATTCTCGATCACTAACCTCCGCATTGCGAAGATGAATCTCAAAACCTCAACAGATTCACCTTCGCATCTCACCACACTCTTCAACCTCACGCTCATCGCTAAGAACCCTAACAACCACCTCGTTTTCTTCTACGAGCCTTTCATTGTTACTGCTTTCTCCGATTCGGTTCAAATCGGGAACGGATCGTTGCCGGCGTTTGATTCCGGGAAGAACAATCAGACGAGTCTCCGGTCGGTGCTGTCGGATTCTCAAGATCTGGATACGGATTCGTTGACTAGTTTGAGATCGGGTTTGAAGAAGAAGAAAGGCTTTCCGGTGGTGATTCAGATGGATACGAAGGTGAAGATGAAGATGGAATGGTTGAAGAGTAAGAAGGTTGGGATTAGAGTGACGTGTGAAGGAATTAGAGGAACTGTTCCCGCCGGAAAATCACCGGCGGTGGCGTCTGTTATCAACTCGGAATGTAAGGTGGATCTTCGAATCAAGATCTGGAAATTCTCCTTTTAGTTCTGTGAGTGATGTTATTATCATCTTTGATTATTATTATTATTGCGGATTATTTGTTAATCAATACTAATAATCAATCCAATCAT
TARGET=719,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0575
PRIMER_LEFT_PENALTY=0.055230
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=CGCTAAGAACCCTAACAACCAC
PRIMER_RIGHT_SEQUENCE=CGTCACTCTAATCCCAACCTTC
PRIMER_LEFT=520,22
PRIMER_RIGHT=817,22
PRIMER_LEFT_TM=60.055
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=0.1196
PRIMER_LEFT_1_PENALTY=0.117281
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=TCATTGTTACTGCTTTCTCCGA
PRIMER_RIGHT_1_SEQUENCE=CGTCACTCTAATCCCAACCTTC
PRIMER_LEFT_1=564,22
PRIMER_RIGHT_1=817,22
PRIMER_LEFT_1_TM=59.883
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=254
PRIMER_PAIR_PENALTY_2=0.1669
PRIMER_LEFT_2_PENALTY=0.164560
PRIMER_RIGHT_2_PENALTY=0.002310
PRIMER_LEFT_2_SEQUENCE=ACCACCTCGTTTTCTTCTACGA
PRIMER_RIGHT_2_SEQUENCE=CGTCACTCTAATCCCAACCTTC
PRIMER_LEFT_2=537,22
PRIMER_RIGHT_2=817,22
PRIMER_LEFT_2_TM=60.165
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=281
PRIMER_PAIR_PENALTY_3=0.2175
PRIMER_LEFT_3_PENALTY=0.215225
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=ATCTGGATACGGATTCGTTGAC
PRIMER_RIGHT_3_SEQUENCE=CGTCACTCTAATCCCAACCTTC
PRIMER_LEFT_3=678,22
PRIMER_RIGHT_3=817,22
PRIMER_LEFT_3_TM=60.215
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=140
PRIMER_PAIR_PENALTY_4=0.2355
PRIMER_LEFT_4_PENALTY=0.055230
PRIMER_RIGHT_4_PENALTY=0.180233
PRIMER_LEFT_4_SEQUENCE=CGCTAAGAACCCTAACAACCAC
PRIMER_RIGHT_4_SEQUENCE=TCGTATCCATCTGAATCACCAC
PRIMER_LEFT_4=520,22
PRIMER_RIGHT_4=761,22
PRIMER_LEFT_4_TM=60.055
PRIMER_RIGHT_4_TM=59.820
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_04613 Ps_contig_mira-and-tgicl-ass_franssen_33938
SEQUENCE=AAATTAGAAAAAACCATACCTATNTTGGGTGGTAGTGGAAACATCCACTTTTTCTTCTCATTCTAATTCCTTTCTCTTCTCTTCACTCCACTCCACTACAAAGCTTCTTCATTTCTCAACACTTTCCTCTCATCTCACCATTCACAATGACCGAACCAACTTCCAAACCTGCAATTAACGGTGCCGCCACAACTAACGGAAATGGAAATGGAAATGGAAATCCACTTCCCGTTAAAGCTCAGCTTTACAATCCAAACCGTCAAGTTTACCGTCCTCAATCACAATACAACCGTCGCCGTCGATCTAACCGTAGCCTCTGCTGTTGCTGCTGTTTCTGGACAATCCTCACAGTTCTCGCCGCCGCACTACTCGTTGCTATTGTCGGTGCTGCACTCTACGTACTCTACCATCCTCACCAACCAGAATTCTCGATCACTAACCTCCGCATTGCGAAGATGAATCTCAAAACCTCAACAGATTCACCTTCGCATCTCACCACACTCTTCAACCTCACGCTCATCGCTAAGAACCCTAACAACCACCTCGTTTTCTTCTACGAGCCTTTCATTGTTACTGCTTTCTCCGATTCGGTTCAAATCGGGAACGGATCGTTGCCGGCGTTTGATTCCGGGAAGAACAATCAGACGAGTCTCCGGTCGGTGCTGTCGGATTCTCAAGATCTGGATACGGATTCGTTGACTAGTTTGAGATCGGGTTTGAAGAAGAAGAAAGGCTTTCCGGTGGTGATTCAGATGGATACGAAGGTGAAGATGAAGATGGAATGGTTGAAGAGTAAGAAGGTTGGGATTAGAGTGACGTGTGAAGGAATTAGAGGAACTGTTCCCGCCGGAAAATCACCGGCGGTGGCGTCTGTTATCAACTCGGAATGTAAGGTGGATCTTCGAATCAAGATCTGGAAATTCTCCTTTTAGTTCTGTGAGTGATGTTATTATCATCTTTGATTATTATTATTATTGCGGATTATTTGTTAATCAATACTAATAATCAATCCAATCAT
TARGET=962,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04614 Ps_contig_mira-and-tgicl-ass_franssen_33942
SEQUENCE=TGTTTACATCATCTTCTATCCAATTCCACGCTCCAATCATTCATCATCGTCTATCCTTCACCACAGCCCACTCCTTATCCTTTCACTCCCACAACCATAACCACACATTCCACACTCCATCATTCCTACGCCCTCCTCCGCTGCTAAACCCCTCCCCTCTTCCCTCCATTTTCACTGTTGCCGCCAAGTCTCCAACACCCGACAAAGCCAGCGAGCAGAAATGGGTCCACGAAGGTCTCATCGCTGAATCCCTCCCCAACGGCATGTTCCGCGTTCTCCTCGACAACGAAGACCTTATCCTCGGCTACATCTCCGGAAAGATTCGAAAGAATTATGTTCGGATCCTTCCCGGAGATAGAGTTAGAGTTGAAGTCAGTCGTTATGACTCCTCCAAAGGACGCATAATTTACCGGATTCGAAATACCACTGGTGGTGGCAGCTCTTAGTTTTTAGGCATGTCTAGTGTTTGTGAAAATGACTCAATCAAATTTGTGAGTTTTGTTTGGGTCCTAATCGGGTTTTGTTTAAAATGGGAGGGATGAAGTAAAAAAGGAANYTTGTCTTATTTTAGTTTTTTTAATTCAACGTTCCTCGGGTATAAAGAACCAACTTTGAGTACATTGTTGTTGTTGTAAGAAAAAGACTAAAAGACTAAA
TARGET=621,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04615 Ps_contig_mira-and-tgicl-ass_franssen_33961
SEQUENCE=ATGACAAGATCACTTGCTTGATTACTATTTAATAGAATAGATGATGCTACAAAGCTCAACCATTATTAAATACAACTACAATCTTAATTGCAGCTGCTACAAGTAATTTAAGTGAAACAGGCAAAAAGGAAAAAAATTGTAGAAAGCTCATTCATCTACAGAAAAATTATGGAAATTCCAGATTCTGTTGTTGGGAACTATAATAATAATATAGTAGAGTACAACCTGGATGGTAGATGGTAGTAATCTAACTGTGAGAAGAGGAACCAAGATTACCATTTGGTACAGTTCCCGAAACCGCATTGCCGTCTGGGTTTGCTTGGTCGTCATTTGCATGGCGAACGAAATCCTCAGGAGTCATATGCGAGCCATGACATGCACAGACGATTCTGATTTGGTTAGTGTTGTACCTGTAAGTAACACCTGATATTGTTCTCCCGTTTGAGTTTGTGGTCGATACCCAAGGCAGATTTGGGTACGCACCACATCCTCCGAATTTCACATCTGCAGCGAGCCCTGGTTTGATATTAGAAAAATCAATGGTTGAAGCTTCAACTTTCGACTGTTCTGATGCATCTTTTCCCCTGAGGTTTGTGCTGCTTCCTTTCACATCTTCAGGTTGAGAAAACGAGCCTTCTTCGGTGACACGCTGTTTTCCATCACTTTTGGCTCGTTCTAGTGGCCTTCCTTCGTATGGCATGGCCTCAGATATATTGTTTAGGTGTAATGCAGCTGAGTTAGTTGGACCTCTCCCAGCAAAGGATGGGTGAAATTGTTGGGGACGTCCCCATGAGCTATCTTTATCCAACATAGGAAGCTGAACATACGGATGTCCAAACATCATTGGCAGGTTTCCATTACTGACAGATTGACCTCCCGCACGTTCACCGGTTGCAGCCGGCATCATATGCATCACTCCAGGTATTTGAGAACTTGGTGCTCCAACAGTGTTTGAATCTTTTACCGAAGTGTAAGGTACATTCATCATATTCATTGGTTGAGCAGAGAAGGAAGCACCATAAGTCATGTTTGCATGTTTAAAATCTTTTTCTGTGGATGCGGTAAACCTCTTCTGCCCGTTGGCACTTGAGTCAGCGACTCCACGAATCTCTTTTGGAGCATCGGAAGAAGCACCGCCAACTCTAACAAATCCTTTGGAACCATCACTATGGTGTGAGAGAGGCCTGGAGGTAGAGTTCTCAGCTTCCGAATCAGCCACGTCTTCATTTTCTGCAGTCGAGCCATCTTCTGTTAAAGAAATATGAGAAGCTCTAGCCCTGTCATGTAAATCACCATGATGAATGTCGCTGTCATGCCTCTTTTGATTACGAATGTCATCAAAAGACATTCTTCGCTTATTTACTACCTCTAACCGTTTATTTTCATCAATTTCAATAGGTTTATTGTGGCTGTTCCCAACAAGAAACCCTCTCCCGTTTAAATTCAAAGAAGCATTGTCTTCTTTGACCTTTGAAAGGTCACTAAAGAAATTCTCCTCATGCTTCGAGGAATCAGTCCTTCGGGTACTAGTGATTGATTCCGGCTTCTGAGGATCAGTATTAAACATGCTCTTAAAATCATCAACCATCTTGCCACCTCTACCAACTTCTTCTGACCTAGTATCCGAAGAGCTTCCATTCTTGCTCTTGGGTTTGGATGATGAACCGCCATAAGATAAACCCAAACTGAGCTCAAGCCCGCTATCATCCTCCATATTGCTATATCTCCAAACCTAAGTCCTGATCTAAATCGTTCCCTTCTCACTCTCTCAACTGATGTGAACAATTCGTTGCTTCCACCCTAATCTAAAGTAGCTCAAGCCAATAGATTATGCCAAATCTATAGCTATAGGATCAACCTCTTTTCAATCTTCAATTTTGAAGCTTTCTTGTCCCAATTCGTTGCTGAATTTTGGAAGAAGAGTGATGATCAATCAAACCCTAAGTTAGGTTTCCATTTTGCAGAGATCCGTGGAAGATTGAGAGTNCCC
TARGET=200,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3607
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=0.139753
PRIMER_LEFT_SEQUENCE=AATTCCAGATTCTGTTGTTGGG
PRIMER_RIGHT_SEQUENCE=CAAATCAGAATCGTCTGTGCAT
PRIMER_LEFT=173,22
PRIMER_RIGHT=396,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=60.140
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.3703
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=0.149344
PRIMER_LEFT_1_SEQUENCE=AATTCCAGATTCTGTTGTTGGG
PRIMER_RIGHT_1_SEQUENCE=ATCAGAATCGTCTGTGCATGTC
PRIMER_LEFT_1=173,22
PRIMER_RIGHT_1=393,22
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.4625
PRIMER_LEFT_2_PENALTY=0.220992
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=AATTCCAGATTCTGTTGTTGGG
PRIMER_RIGHT_2_SEQUENCE=AACGGGAGAACAATATCAGGTG
PRIMER_LEFT_2=173,22
PRIMER_RIGHT_2=441,22
PRIMER_LEFT_2_TM=60.221
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=0.4640
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.243031
PRIMER_LEFT_3_SEQUENCE=AATTCCAGATTCTGTTGTTGGG
PRIMER_RIGHT_3_SEQUENCE=ACGGGAGAACAATATCAGGTGT
PRIMER_LEFT_3=173,22
PRIMER_RIGHT_3=440,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.757
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.5344
PRIMER_LEFT_4_PENALTY=0.394672
PRIMER_RIGHT_4_PENALTY=0.139753
PRIMER_LEFT_4_SEQUENCE=CCAGATTCTGTTGTTGGGAACT
PRIMER_RIGHT_4_SEQUENCE=CAAATCAGAATCGTCTGTGCAT
PRIMER_LEFT_4=177,22
PRIMER_RIGHT_4=396,22
PRIMER_LEFT_4_TM=60.395
PRIMER_RIGHT_4_TM=60.140
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_04616 Ps_contig_mira-and-tgicl-ass_franssen_33972
SEQUENCE=AAGATTGGATTAAACAACACGGAGGTTGTTAATGGAGTAGTAGGTATGAATAAATGATCTGAGTTAATTAAGTAGAGAAAGTTAAAGGAAAAAGAAAAGAAAAGGAAAAAGACGAGGATGAGTCGGAGAGGAGATGAAGAATACGATTATCTGTTCAAAGTGGTACTGATCGGCGATTCCGGTGTCGGTAAATCAAATCTTCTTTCCCGATTCACTCGCAATGAATTTTGTCTCGAATCCAAATCCACCATCGGCGTCGAATTCGCCACACGCACACTTCAGGTCTCTCTTTCTTACTATCACCCTCCAAGGGAATGCCAAGATTTTAGCCAATGGTGTCTTGGCA
TARGET=90,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1392
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=ATTGGATTAAACAACACGGAGG
PRIMER_RIGHT_SEQUENCE=CCGATCAGTACCACTTTGAACA
PRIMER_LEFT=3,22
PRIMER_RIGHT=172,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.1518
PRIMER_LEFT_1_PENALTY=0.108929
PRIMER_RIGHT_1_PENALTY=0.042918
PRIMER_LEFT_1_SEQUENCE=ATTGGATTAAACAACACGGAGG
PRIMER_RIGHT_1_SEQUENCE=TTCTTCATCTCCTCTCCGACTC
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=140,22
PRIMER_LEFT_1_TM=60.109
PRIMER_RIGHT_1_TM=59.957
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=0.1638
PRIMER_LEFT_2_PENALTY=0.108929
PRIMER_RIGHT_2_PENALTY=0.054900
PRIMER_LEFT_2_SEQUENCE=ATTGGATTAAACAACACGGAGG
PRIMER_RIGHT_2_SEQUENCE=GTGAATCGGGAAAGAAGATTTG
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=214,22
PRIMER_LEFT_2_TM=60.109
PRIMER_RIGHT_2_TM=59.945
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.2601
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.054900
PRIMER_LEFT_3_SEQUENCE=ACACGGAGGTTGTTAATGGAGT
PRIMER_RIGHT_3_SEQUENCE=GTGAATCGGGAAAGAAGATTTG
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=214,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=59.945
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=199
PRIMER_PAIR_PENALTY_4=0.2690
PRIMER_LEFT_4_PENALTY=0.108929
PRIMER_RIGHT_4_PENALTY=0.160072
PRIMER_LEFT_4_SEQUENCE=ATTGGATTAAACAACACGGAGG
PRIMER_RIGHT_4_SEQUENCE=CTTCATCTCCTCTCCGACTCAT
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=138,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=59.840
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=136
=
PRIMER_SEQUENCE_ID=SSR_04617 Ps_contig_mira-and-tgicl-ass_franssen_33993
SEQUENCE=CCATTTGCGGTGACATATCTCGGAGCTTCTCTCATGGTAGTTTACCTCCCAATAGCATTCATTAAGGATTGGTTCTATAACTTGCTTAAACACCGGTCTTCTAAAAGTGGAAAAAATGCAGAAAGCGACGAGGACAAGTTTCCTATCAAAATTAGTTCTCCACTCAAAGGAAATGGAGTGCAGAAAAACTTTGAAGTGGAACTCCCGAACATTGTTCGAAAAGAAAGTGATATAGATCTTTCAAGTCTTGCAGAGATAGCGCCATTGGTGGATAAATATAATGAAACTGGTGTGGCAAAGGTAGAAAAAGAACTTACTGCAAAAGAAATCGCTACTTATGGATTTTACATTGCACCTATCTGGTTTGTAACAGAGTATCTATCAAATGCCGCGCTTCGCCCGTACAAGTGTCGCGAGTACAACAGTATTGTCATCTACTTCAGGACTCTTCACTCTTTTCATCGGTGTGTTAATGGGTCAAGACACTTTGAATGTATCCAAAGTAGTTGCTGTCTTGGTCAGCATGGCCGGGGTTGCCATGACAACTCTGGAAAAAACATGGGCCGCAGATGACTCGCAATTGAATGCTTCCAATGGACGGCGCTCTCTTGTTGGAGATCTTTTTGGCCTTCTCTCAGCCATGTCATATGGTCTATTTACTGTTCTTCTAAAAAAGTTTTCTGGTGAAGAAGGTGAACGTGTTGACGTGCAAAAGCTGTTTGGATATATTGGATTGTTTACACTTGTAGCACTATGGTGGCTTATCTGGCCGTTGTCGGCCTTAGGAATTGAACCCAAGTTTGCGATTCCTCAGTCTGCCAAAATGGATGAAGTGGTTCTTGCAAATGGATTTGTTGGAAGTGTTCTCTCAGACTACTTCTGGGCACTCTGTGTTGTATGGACAACTCCCCTCGTGGCCACTTTGGGCATGTCACTCACTATTCCTCTTGCTATGTTGGCTGATATGGTAATCCACGGTCGGCATTATTCAGCACTATACATTCTTGGCTCAGTTCAGGTATTTGCAGGTTTTGTGATAGCTAATCTTTCAGATTGGACGACAAAGAGGTTGGGATTATAGCACATGATGTTTTGTTGATTAGTTTGTGGACAACACTTATTCATTTTTTGGTTGATTTATCCTTCCACTTTTCTGATTTAGTGTCATAGCACGAGTCAATCAGTATCTGTTGAAAACCAAAGTCTTAATCCTGTTTTGTTTTTTTGAAATTTTCAAGGAAATCAACAAAAGAGTGTTGTTTCCATGATGTATTTGTTCTGTGTATATGTTTTTTTATTTTATTTTATTTTATTTTAGAGTGAGTACTTTGTTGTAATCATGTCTTTGAATAGGTGCTAGCACAATTTGTGGCTACATCAGTAAATGGGTTTTTAAATGAAAGAAATTGAGAATTTCACTTGAATAAGGGT
TARGET=1293,25
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.6666
PRIMER_LEFT_PENALTY=1.182682
PRIMER_RIGHT_PENALTY=1.483928
PRIMER_LEFT_SEQUENCE=AGTGTCATAGCACGAGTCAATCA
PRIMER_RIGHT_SEQUENCE=AACCCATTTACTGATGTAGCCA
PRIMER_LEFT=1160,23
PRIMER_RIGHT=1390,22
PRIMER_LEFT_TM=59.817
PRIMER_RIGHT_TM=58.516
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=2.6800
PRIMER_LEFT_1_PENALTY=1.196037
PRIMER_RIGHT_1_PENALTY=1.483928
PRIMER_LEFT_1_SEQUENCE=GTGTCATAGCACGAGTCAATCA
PRIMER_RIGHT_1_SEQUENCE=AACCCATTTACTGATGTAGCCA
PRIMER_LEFT_1=1161,22
PRIMER_RIGHT_1=1390,22
PRIMER_LEFT_1_TM=58.804
PRIMER_RIGHT_1_TM=58.516
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=3.2290
PRIMER_LEFT_2_PENALTY=1.048100
PRIMER_RIGHT_2_PENALTY=2.180916
PRIMER_LEFT_2_SEQUENCE=TGTCATAGCACGAGTCAATCAG
PRIMER_RIGHT_2_SEQUENCE=GCTAGCACCTATTCAAAGACATGA
PRIMER_LEFT_2=1162,22
PRIMER_RIGHT_2=1360,24
PRIMER_LEFT_2_TM=58.952
PRIMER_RIGHT_2_TM=59.819
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=3.3021
PRIMER_LEFT_3_PENALTY=1.048100
PRIMER_RIGHT_3_PENALTY=2.253992
PRIMER_LEFT_3_SEQUENCE=TGTCATAGCACGAGTCAATCAG
PRIMER_RIGHT_3_SEQUENCE=TGCTAGCACCTATTCAAAGACA
PRIMER_LEFT_3=1162,22
PRIMER_RIGHT_3=1361,22
PRIMER_LEFT_3_TM=58.952
PRIMER_RIGHT_3_TM=57.746
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=3.3636
PRIMER_LEFT_4_PENALTY=1.182682
PRIMER_RIGHT_4_PENALTY=2.180916
PRIMER_LEFT_4_SEQUENCE=GTGTCATAGCACGAGTCAATCAG
PRIMER_RIGHT_4_SEQUENCE=GCTAGCACCTATTCAAAGACATGA
PRIMER_LEFT_4=1161,23
PRIMER_RIGHT_4=1360,24
PRIMER_LEFT_4_TM=59.817
PRIMER_RIGHT_4_TM=59.819
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_04618 Ps_contig_mira-and-tgicl-ass_franssen_33995
SEQUENCE=AGCGRGNANAAACACCAACACAAATTAACAATAGTACTAACAACCTTATTATTCTCTAACACTAACTAACAATAACAACAATGGCAGTTTCAACTAGTTTTTTCTCTAGTGCAAAATCAGAACCTTTCTTCCTTAAACATTGTTCTTCTTCTCCCTCTCCTTCCTCGTTCTCTTATCTTACAACTCATTTATCATTTTACGGTAGAAACAGAAAGAGCCTTGTTCAGAGTCAGAAAGGTTCAATTATTCGCTGTGACGCTTCTTCTTCTTCTGATGCTAACTCTGTCTCTGCTCTACAACAGCTTAAAACTTCTGCTGCTGATAGATATACGAAGGAAAAGAGCAGCATTGTGGTGATTGGGTTGAGTGTGCATACTACACCGGTGGAACTGCGTGAGAAACTTGCTATACCAGAAGCTGAATGGCCAAGAGCCATTGGTGAGCTTTGTGGTTTGAATCATATTGAAGAAGCCGCTGTTCTTAGCACATGTAACAGAATGGAGATTTATGTTGTTGCACTTTCTCAGCATCGTGGCCGTCAAAGAAG
TARGET=259,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04619 Ps_contig_mira-and-tgicl-ass_franssen_33996
SEQUENCE=CTTTATTGGATATTGTGAAGAAGAAGAACCTTTCACCAGAATGTTGGTTTTCGAATATGCACCAAATGGGACGCTATTCGAGCATTTACACGTAAAAGAAGCCGAGCATTTGAACTGGGGACCGAGACTTAGAATTGCGACAGGAATGGCTTACTGCCTACAATACATGCATGGACTGGACCCTCCTGTGGCCCTTATCAACCTGAGTTCTTCAACTGTCCATTTAACTGATGATCATGCAGCAAAAATCTCGGATTTGAGTTTCTCCCACGAAAAAGATTCATCTGAAAAGAAACCTGATGGTAGAAAAGACATTGATATAGATATGATGGATTCAACAAGTAGAGCGAGTAATGTTTACAGCTTTGGTGTTTTGTTATTCGAGAT
TARGET=17,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04620 Ps_contig_mira-and-tgicl-ass_franssen_34018
SEQUENCE=AAAGGGGAAGTCTTTTCTCTTTCTCAGTTTCTGTAATCATTCATCAGAAGAAACATATCATAGATCTCTCTTTCAATCATTCGTAATTCCATTTCACAATTCAATCCCTAATTTTCTAGGGTTTCCGATCTTTAACCTTCTTCTTCTTCTTCTTCNTATGTTTTCTCTGACCTAAATTAATGGCGTCGATTCGACGGACGCTATCTCCGGCTTATCACGACCGTCATCACTACATAAACACCGTCTTTCGTCTTCCTCTCCGTCACACAACTTCCTTCTCAAC
TARGET=137,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5086
PRIMER_LEFT_PENALTY=0.182633
PRIMER_RIGHT_PENALTY=0.325919
PRIMER_LEFT_SEQUENCE=CCCTAATTTTCTAGGGTTTCCG
PRIMER_RIGHT_SEQUENCE=TTGAGAAGGAAGTTGTGTGACG
PRIMER_LEFT=105,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=60.183
PRIMER_RIGHT_TM=60.326
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.8944
PRIMER_LEFT_1_PENALTY=0.182633
PRIMER_RIGHT_1_PENALTY=0.711786
PRIMER_LEFT_1_SEQUENCE=CCCTAATTTTCTAGGGTTTCCG
PRIMER_RIGHT_1_SEQUENCE=AAGTTGTGTGACGGAGAGGAAG
PRIMER_LEFT_1=105,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=60.183
PRIMER_RIGHT_1_TM=60.712
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=168
PRIMER_PAIR_PENALTY_2=0.8944
PRIMER_LEFT_2_PENALTY=0.182633
PRIMER_RIGHT_2_PENALTY=0.711786
PRIMER_LEFT_2_SEQUENCE=CCCTAATTTTCTAGGGTTTCCG
PRIMER_RIGHT_2_SEQUENCE=AAGGAAGTTGTGTGACGGAGAG
PRIMER_LEFT_2=105,22
PRIMER_RIGHT_2=276,22
PRIMER_LEFT_2_TM=60.183
PRIMER_RIGHT_2_TM=60.712
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=0.8944
PRIMER_LEFT_3_PENALTY=0.182633
PRIMER_RIGHT_3_PENALTY=0.711786
PRIMER_LEFT_3_SEQUENCE=CCCTAATTTTCTAGGGTTTCCG
PRIMER_RIGHT_3_SEQUENCE=AGAAGGAAGTTGTGTGACGGAG
PRIMER_LEFT_3=105,22
PRIMER_RIGHT_3=278,22
PRIMER_LEFT_3_TM=60.183
PRIMER_RIGHT_3_TM=60.712
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=1.2310
PRIMER_LEFT_4_PENALTY=0.182633
PRIMER_RIGHT_4_PENALTY=1.048411
PRIMER_LEFT_4_SEQUENCE=CCCTAATTTTCTAGGGTTTCCG
PRIMER_RIGHT_4_SEQUENCE=GAAGACGAAAGACGGTGTTTATG
PRIMER_LEFT_4=105,22
PRIMER_RIGHT_4=254,23
PRIMER_LEFT_4_TM=60.183
PRIMER_RIGHT_4_TM=60.048
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_04621 Ps_contig_mira-and-tgicl-ass_franssen_34023
SEQUENCE=TAAATAACTCCAAAGAACAAGCTCAGTAAAANTAANTTNCTACTCCTTTGGTCTTTGATTGTTTTTTCGATTTTTGTTGAACAAGCCTTATCAAATAGAGATGGAAAGAGTATCATATATGCACATCTGTACAAATTAAATGTCATTGCAAATACTAAAACCATTCAAAACTCCAGTAACTGTAGGAAGCAAGAGAGTTGACAGTGGTGCAGGCAGACAAGAAGACCAGATTCTACACTTTGGTTTGCAAAGCAGTTGACATGTTTCCGTCGCAATGTTCTTCAAGCTGCCAGTGGTTCCTCCATAACTTTTCTTTTTATAGGTTCTTTAGCCTCAGTGGCAACTGGTGCCTTGGTTGGGACATCAGGAAGGTCCAATTTTTCATCAATATCTTCAGAAACTGTGGTAGGAACTTCAGGAAGATCATCGACAGCAAGCTGGGTTTCCAAGTTCTCAAACTCTGCCAAAATCTCCTCTTCATCTTCTGCTGATAATTGCTCCCCAAGTACCGCATTGATTTCATCTTGATAAGCTTTAGCTTCAGCAGTGTCATCCATAAGTTTTTGAACATCCTCAATGTTTATCTCACTTTGTATGGCTTTCATCGCTTCATTTCCAGCCTTCAAACTTTCAAAAACAGACTTCTGCTTGCTTGCTAGTTCAATATCTGCTAATTGTTGCTCAACATTTATAAGCCAGGTATCAACTTGCTTCAACAATTCTTCTTGTGTCTTTTTTTCTTTTAACGCGAGCAAGGCCCTGTCTTTCTTCTTTTCACGAATCAAGTCACGTGCAGCTTGCTTTTCTGCTTCAATAACAGCATCAAGCTTTTGTTGATATTGGGCAAGTTTGCGTCTCTGGGTCTTGAGAGCTAGAATTGCACGATCCACGTCTGTTACCTTAGGTTTCTTCACGAATATATTCCCCATCGATTTCGACTTTGCGGTGTTGATAAGGATGTTTTATAATCTGGGCATGGAGTTGGATTGAATCGAATCGATCATAGAGAGGAATAATAGTGATGATGATCGAATTCAGAAGATGAGAATTGAAGGTATATTGTATTTGGTTTTTAGTTTTTTCAGTYGGAAAAAACTTCGAAAGAGGGTTTTGACGTTTCGAACAGCTGACAACCTNNCCNCACT
TARGET=646,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04622 Ps_contig_mira-and-tgicl-ass_franssen_34024
SEQUENCE=TGTATGTTTGCATGAAGAGGATCACGGGATTCTCTGGAAGCATACAAGACGTGGAGAACGTGGTTTAGCAAGAACGTACGAGGTCTAGAAGACTTTCAGTTTCTTTTGTATGCACAGTGGCCAACTATGAGTACGCGTTTTTTTGGCATTTTTATCAGGATGGAAAGATTGAAGCTGAAGTCAAGCTGACTGGAATTCTCAGCTTAGGAGCTTTGATGCCTGGAGAATATCGAAAATATGGAACCATGATTGCACCAGGTCTATATGCTCCAGTTCATCAACACTTTTTTGTTGCTCGCATGGACATGTCTGTTGATTCTAGACCGGGTGAAGCTTTGAATCAGGTTGTGGAGGTCAATATGAAAGTTGAAGAACCTGGTGAGAATAATATTCACCATAATGCGTTCTATGCTGAAGAGACTTTGCTCAAATCTGAATCCGAAGCAATGCGGGATTGCAATCCTTTGACTGCTCGACATTGGGTTGTTAGGAACACGAGAACCGGCAACAGAACTGGACAATTAACAGGCTACAAGCTGGTACCAGGCTCAAACTGCTTGCCATTGGCAGGTTCTGAGGCCAAATTTTTAAGAAGAGCTGCTTTCTTGAAGCATAATATGTGGGTAACATCATATTCACGTGATGAAATGTTTCCTGGGGGAGAATTTCCCAATCAAAATCCGCGAGCGGGTGAAGGACTAGCTACATGGATTAAGCAAAACCGGCCTTTGGAAGAAACCAATATAGTTCTTTGGTCCTCTATCTCTCTTCTCTCTCAACTCACACACACTAACACAACACACGCGCGACACACACACA
TARGET=809,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04623 Ps_contig_mira-and-tgicl-ass_franssen_34037
SEQUENCE=GTTTAAAGTCTATTCTACTTGTAATAACAATAACAATAGTCTTACAACAAACATCACCAAATAGTACGAGCAGAAAAGGTAATAAGAGAAACAACAACATTGACGACGACGACAACGACAACAACAACAAAAATCCCTCCAAAGATCATCTGATCTTACACAAAAGTTTGGCAAGTAGACTGATAAAATGGGAGAGAAAATTATTACACATTCAAATGTGTTATTCAAATGTGCTTCTTGATATATGTATTTCTGTAATAAATTTAAGTACTTGTCACAACTCCATCGAAAATAATAAAACATAGCAATGTGAAACCTCATTGTAGTCATCTCCTGATAACTTCTCTTCACGGTGTTGGGAACAATAGCAAACAGGGTCTTCGGAGCAGAAAAGAGTATTATTCCCAAATGCAAAAAAGGATTCTGGTTCTGACACGAAAAAAAGTTTGACGCATAAATAAGCACGAGAAATAGACATGTGGACGGGCATGTGCATAATGGACTTGCAGATTCTCTTCAAGTTATTCAAAGAGCAAGGTGCGTGTATGTTGGCCATGGTTTTATTCAAGATCAAAGAGATGGCCACGAAAAGACTATATGGACACAAAGACAATTGATTGGTGCAATGAGTGTTGGGTCTGAACTGGGATAACTTAAAAATAAAGACTAATTTTGAGACTGGCCGATTGAAAAATTGAGATAAAATGGAGAGATAATGAGGAGTACATTCACATTGCTTAGCTTGAGGTGTGAGAGACAGTGCTACTTGTGGTGGCACTGGGAGTTGCTAAAGAACTGGAACTCGGAGCAGATGGCTGGCTACTTGATTGAGAAACCGGTACGACAGTTGACAGAGTTAACCCAGCTGATGCAGTCGACGTGCTCATGGATGATGACATTCTGGATGAAGCAGACTCTTTGCTGGATCCCGAGGCAAGTTCAGGCCTAAACTCGGATGATGAGGATGATGGAGCAAGGGTGCCAATGGAGGAACCAACCGGATAGGGTGCAACTGGCATATCAGCTAGAGAAGAAGCAGATGGACTGTAACTCAGGGATCCCATAGGATGATCAAATCTGCATGCAGATCCAAACTTACAAATTCCACGCTGCGTATAATGAGTGCATGGTTGTCGCCCCCGGGCGCAAAGGAAGACCGGCAGGACTAAAGATCACTTTCGGTGCACCAGTGTCTCGCGGATGATGATATCTACACGAAGAACCAAATTTACACTCCCCCGTTTTCATGTAATGATGACAATCCTGCTGANCATTTTTTCTGAGCAAAATGAGAGT
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7937
PRIMER_LEFT_PENALTY=1.696913
PRIMER_RIGHT_PENALTY=0.096820
PRIMER_LEFT_SEQUENCE=CATCACCAAATAGTACGAGCAGA
PRIMER_RIGHT_SEQUENCE=TCAGATGATCTTTGGAGGGATT
PRIMER_LEFT=51,23
PRIMER_RIGHT=152,22
PRIMER_LEFT_TM=59.303
PRIMER_RIGHT_TM=59.903
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=2.1064
PRIMER_LEFT_1_PENALTY=1.696913
PRIMER_RIGHT_1_PENALTY=0.409511
PRIMER_LEFT_1_SEQUENCE=CATCACCAAATAGTACGAGCAGA
PRIMER_RIGHT_1_SEQUENCE=GATCAGATGATCTTTGGAGGGA
PRIMER_LEFT_1=51,23
PRIMER_RIGHT_1=154,22
PRIMER_LEFT_1_TM=59.303
PRIMER_RIGHT_1_TM=60.410
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=104
PRIMER_PAIR_PENALTY_2=2.1384
PRIMER_LEFT_2_PENALTY=2.041548
PRIMER_RIGHT_2_PENALTY=0.096820
PRIMER_LEFT_2_SEQUENCE=CAAACATCACCAAATAGTACGAGC
PRIMER_RIGHT_2_SEQUENCE=TCAGATGATCTTTGGAGGGATT
PRIMER_LEFT_2=47,24
PRIMER_RIGHT_2=152,22
PRIMER_LEFT_2_TM=59.958
PRIMER_RIGHT_2_TM=59.903
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=106
PRIMER_PAIR_PENALTY_3=2.1788
PRIMER_LEFT_3_PENALTY=2.081990
PRIMER_RIGHT_3_PENALTY=0.096820
PRIMER_LEFT_3_SEQUENCE=ACATCACCAAATAGTACGAGCAGA
PRIMER_RIGHT_3_SEQUENCE=TCAGATGATCTTTGGAGGGATT
PRIMER_LEFT_3=50,24
PRIMER_RIGHT_3=152,22
PRIMER_LEFT_3_TM=60.082
PRIMER_RIGHT_3_TM=59.903
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=2.2771
PRIMER_LEFT_4_PENALTY=1.696913
PRIMER_RIGHT_4_PENALTY=0.580189
PRIMER_LEFT_4_SEQUENCE=CATCACCAAATAGTACGAGCAGA
PRIMER_RIGHT_4_SEQUENCE=CAGATGATCTTTGGAGGGATTT
PRIMER_LEFT_4=51,23
PRIMER_RIGHT_4=151,22
PRIMER_LEFT_4_TM=59.303
PRIMER_RIGHT_4_TM=59.420
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_04624 Ps_contig_mira-and-tgicl-ass_franssen_34037
SEQUENCE=GTTTAAAGTCTATTCTACTTGTAATAACAATAACAATAGTCTTACAACAAACATCACCAAATAGTACGAGCAGAAAAGGTAATAAGAGAAACAACAACATTGACGACGACGACAACGACAACAACAACAAAAATCCCTCCAAAGATCATCTGATCTTACACAAAAGTTTGGCAAGTAGACTGATAAAATGGGAGAGAAAATTATTACACATTCAAATGTGTTATTCAAATGTGCTTCTTGATATATGTATTTCTGTAATAAATTTAAGTACTTGTCACAACTCCATCGAAAATAATAAAACATAGCAATGTGAAACCTCATTGTAGTCATCTCCTGATAACTTCTCTTCACGGTGTTGGGAACAATAGCAAACAGGGTCTTCGGAGCAGAAAAGAGTATTATTCCCAAATGCAAAAAAGGATTCTGGTTCTGACACGAAAAAAAGTTTGACGCATAAATAAGCACGAGAAATAGACATGTGGACGGGCATGTGCATAATGGACTTGCAGATTCTCTTCAAGTTATTCAAAGAGCAAGGTGCGTGTATGTTGGCCATGGTTTTATTCAAGATCAAAGAGATGGCCACGAAAAGACTATATGGACACAAAGACAATTGATTGGTGCAATGAGTGTTGGGTCTGAACTGGGATAACTTAAAAATAAAGACTAATTTTGAGACTGGCCGATTGAAAAATTGAGATAAAATGGAGAGATAATGAGGAGTACATTCACATTGCTTAGCTTGAGGTGTGAGAGACAGTGCTACTTGTGGTGGCACTGGGAGTTGCTAAAGAACTGGAACTCGGAGCAGATGGCTGGCTACTTGATTGAGAAACCGGTACGACAGTTGACAGAGTTAACCCAGCTGATGCAGTCGACGTGCTCATGGATGATGACATTCTGGATGAAGCAGACTCTTTGCTGGATCCCGAGGCAAGTTCAGGCCTAAACTCGGATGATGAGGATGATGGAGCAAGGGTGCCAATGGAGGAACCAACCGGATAGGGTGCAACTGGCATATCAGCTAGAGAAGAAGCAGATGGACTGTAACTCAGGGATCCCATAGGATGATCAAATCTGCATGCAGATCCAAACTTACAAATTCCACGCTGCGTATAATGAGTGCATGGTTGTCGCCCCCGGGCGCAAAGGAAGACCGGCAGGACTAAAGATCACTTTCGGTGCACCAGTGTCTCGCGGATGATGATATCTACACGAAGAACCAAATTTACACTCCCCCGTTTTCATGTAATGATGACAATCCTGCTGANCATTTTTTCTGAGCAAAATGAGAGT
TARGET=118,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1529
PRIMER_LEFT_PENALTY=0.057411
PRIMER_RIGHT_PENALTY=0.095538
PRIMER_LEFT_SEQUENCE=AAACAACAACATTGACGACGAC
PRIMER_RIGHT_SEQUENCE=TGTTTGCTATTGTTCCCAACAC
PRIMER_LEFT=88,22
PRIMER_RIGHT=373,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.1695
PRIMER_LEFT_1_PENALTY=0.073989
PRIMER_RIGHT_1_PENALTY=0.095538
PRIMER_LEFT_1_SEQUENCE=AGAAACAACAACATTGACGACG
PRIMER_RIGHT_1_SEQUENCE=TGTTTGCTATTGTTCCCAACAC
PRIMER_LEFT_1=86,22
PRIMER_RIGHT_1=373,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.1000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.5313
PRIMER_LEFT_2_PENALTY=0.057411
PRIMER_RIGHT_2_PENALTY=0.473868
PRIMER_LEFT_2_SEQUENCE=AAACAACAACATTGACGACGAC
PRIMER_RIGHT_2_SEQUENCE=AGACCCTGTTTGCTATTGTTCC
PRIMER_LEFT_2=88,22
PRIMER_RIGHT_2=379,22
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=59.526
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=292
PRIMER_PAIR_PENALTY_3=0.5479
PRIMER_LEFT_3_PENALTY=0.073989
PRIMER_RIGHT_3_PENALTY=0.473868
PRIMER_LEFT_3_SEQUENCE=AGAAACAACAACATTGACGACG
PRIMER_RIGHT_3_SEQUENCE=AGACCCTGTTTGCTATTGTTCC
PRIMER_LEFT_3=86,22
PRIMER_RIGHT_3=379,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.526
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=0.5908
PRIMER_LEFT_4_PENALTY=0.057411
PRIMER_RIGHT_4_PENALTY=0.533373
PRIMER_LEFT_4_SEQUENCE=AAACAACAACATTGACGACGAC
PRIMER_RIGHT_4_SEQUENCE=CGAAGACCCTGTTTGCTATTGT
PRIMER_LEFT_4=88,22
PRIMER_RIGHT_4=382,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=60.533
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_04625 Ps_contig_mira-and-tgicl-ass_franssen_34044
SEQUENCE=GGTTTTTAATTCTAATAGTAGTTGTTACTATAATATTTTCTCAAACTCATGCTTTTGTTTTCCAAATATATTATTGATTTTAAACATCAGTACCAATTTAACATCACAGAAATATCAATGAAGATAGCCAAACATGGAGTGGTTTGATAGAACCAAACTACATAAACAAACAGCCCTTGGCAAGTTAAGAAAAAACACATTGTAGCCTCATTAAGGTCTACCTTTCATAACTCATGATTATTACAAAGCATCTTCCATGCTCAAGGCTTGAACTTCCTGTATGGTCTGCCATTATCTTCATCTTGCATATGGAATTCCTTGACGATGCCTTTGTAGACTAGTACAGGAACAGCGAGACCGAAGATACCAACAAGAAGAAGGTTCCGGCGAGACCAACGGAAATTGTGCTCGAGATTCTCTCTAACAGCGCCCCATTCTTCAATGTATTTGTTCTTGTTTGCTTCCATTCCTCCACCCATTTTTTCTTCTTCTTCTTCCTTTCGATCTCTCTCTCTCYCTTCCTCTTCTCTCACATCACTCACTTCTATTACTCTCCTCTCTGTAAAT
TARGET=483,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04626 Ps_contig_mira-and-tgicl-ass_franssen_34044
SEQUENCE=GGTTTTTAATTCTAATAGTAGTTGTTACTATAATATTTTCTCAAACTCATGCTTTTGTTTTCCAAATATATTATTGATTTTAAACATCAGTACCAATTTAACATCACAGAAATATCAATGAAGATAGCCAAACATGGAGTGGTTTGATAGAACCAAACTACATAAACAAACAGCCCTTGGCAAGTTAAGAAAAAACACATTGTAGCCTCATTAAGGTCTACCTTTCATAACTCATGATTATTACAAAGCATCTTCCATGCTCAAGGCTTGAACTTCCTGTATGGTCTGCCATTATCTTCATCTTGCATATGGAATTCCTTGACGATGCCTTTGTAGACTAGTACAGGAACAGCGAGACCGAAGATACCAACAAGAAGAAGGTTCCGGCGAGACCAACGGAAATTGTGCTCGAGATTCTCTCTAACAGCGCCCCATTCTTCAATGTATTTGTTCTTGTTTGCTTCCATTCCTCCACCCATTTTTTCTTCTTCTTCTTCCTTTCGATCTCTCTCTCTCYCTTCCTCTTCTCTCACATCACTCACTTCTATTACTCTCCTCTCTGTAAAT
TARGET=505,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04627 Ps_contig_mira-and-tgicl-ass_franssen_34047
SEQUENCE=TCAACRCNNNNTACGGGGGAGCCTACACAAACTCACACCGTATACTCCTCTCCTTTTCCATCTTCACAATTTCCTCTTACAAAAAAATGGAAGCAAACAGAAAGAGAAACCGAGGACTCTTGAAAGGAAAGCTATCACCTTTCTTTAGAACAACAAAACCTTCAACAACAACATTAGAATTCACAACAACCAACGTTAAACCAAATCACTCTTCACCTTCCCCTGCCTCTCATGTTGTTGTAGTCCACAAAGATTATGCCAAGCCAAGCCCAAAAGTTTCTATTGTGGTAACAGATTGTTGGAGTGATAATTCTCCAACGGATGAATGTGTTGACATGAAAGCTTCAATTTATATATCTATGGTTCAACAACGTTTGAAAGATGAACAAAAACAAGTTCATCAGAATTGATGAAACATTGTTGTATTTTAGATATAGTATTATGGTTGTTGCTAGATGTGGTACTGTTTAATAAATAGCATGTTTTTTTGTTGTTGTCATTTTGACACTTTGTGTAGAGATAATTATGTCATGTTATAGTTTGTTTAATTAAAATAACAAGTTTGTAAATGTTTCCCCTTTAAAGGTCAATCTTTTGNTTATTAANTTTCTCTCTTCTCTAGCAAACTAACAATGGTAGATCTATGAAAAAAAGTTATGGGGCTCTTGTCTCCACAAAGGAAACAAAAATAATAATAATTATTT
TARGET=688,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04628 Ps_contig_mira-and-tgicl-ass_franssen_34048
SEQUENCE=TTTACTAGAATAGAATTATTGAAATGTCTCTACTGTTATTAGTCAAGATTCCTTAGATTAAATTAATTTGGTTAAGGTGGACAATTGAATGAAAAAAAGTAGATGGCTGAGACACTTACTCGCATTCTAAACTCAATCAGCATAAGATACAATTTCAACAATAGGTAACCAGCAACAATGTGAAAACAAAAAATTGACAATAATCTCCATCTCACAAAAATGGTTCCCAACAGTTTTTCTCTTCCCACATACCTAAATCCATCTCAGGTTTTTATAAAAGGTTGTAGCTGTCTCTTATCTGTCTTTCTTTCAACTCAGAATGGGTGCAGGAGGGACTTCAAGGGTGATTCCAGCTTGGATTTGGCCCCAGCCGATAAGACGCCAATGCTTCTCGACACGTCTACTTAGAGCAATCTTCTCACCCTTGCTGGTGCACACAGGGGAAGTAAGTTGCAATTTCGCCAAGTCATTCTTCACAGCAACCACTCTGGCACCTGTTGACATTGATCCTATGTTCAACATAAGCATCTCTCCTTTCGCCAGTTTTGCGACTTTTCCTTGTCTCTCCGAACCTTTTGTCCGGACACCAAGAAGCCGTCGAAGCAAGAAAAAGTTTACCTCAAGTTCCACAAAAACCTCGGGCAGTGATCCAACCTCTCCAAGAACTTGCCCTACCAACCTGTCAGCACGAGTCAAGGTGGGGTCCATTGTTGTTCCAACACCAATAAGGCCTCCAGGAACTGCAAACTGAAGCTCATTTTGTTCAGCATATAATGAAACTATTCTCGAGTAGATAGGTGTGCATCGGATGTTTCCACTCTCGTCTTTAACAACTATTCCAGGTCGAACTTCAATGAATTGGTTGACCTTTAAAACACCCCTTAGAATGCTTCCACCAGCTACACCTCCTTTTATTTCATCAACCTCATAACCGGGTTTATTGACATCAAATGACCGAATTACTATCATATTAGGTGGAGAAATGAAATTCCGTTCAGGGATAGGGATCTTTTTCACAATGTACTCACACACAACATCAATATTATACTTCAATTGTGCTGAAATAGGTACTACTGGTGCACTATCAGCAACAGTTCCTTGAATAAACTTCTGAATTGCTTCATGCTGGTTGATTGCCACATTTTCTTGAATCAAGTCTACCTTGTTCTGAAGGATAATTATGTGCTGTAGACGCATAATTTCCACGGCAGCTAAATGCTCAGAAGTTTGTGGTTGCGGGCAGCTTTCATTAGCAGCAATGAGTAGCAAAGCACCGTCCATGATTGCTGCTCCATTAAGCATAGTAGCCATGAGAATATCATGTCCTGGGCAATCAACAAAAGAAACATGTCTCAGCAATTTCATCTTGGAATTTTCAAACCCTAGTACGTCACACAAAGGATTATCTTCCTTTCCACTTCCATAAGCCTTGTAGGACATAGGCCTTGGACACCGCTCATCTTCACACTTGTATATTTTTGCATTTGCATAACCAAGCTTGATTGTAATGTTACGTTCCAACTCATTTTTAAAACGCACAGTCTGAACACCTGATATTGCTTTCACAACAGTCGACTTTCCATGTGCCACATGACCTATGGTGCCAATATTGATAGTAGCCTGGCGAGATATGACTTCAGGAGAAAGTGGATGCAGTTTTGTCACATCCAACTTACTCAGGTCTTGTTCCATCAACCCCCTTCCGCGACTATTTTGGCTGCAGAGAAGGAGATCGTTAAAGTTTGTCAGGTGAGTTCTTGGTTACACGATTAGTTCCTTTCTTCTTCTTCTT
TARGET=1779,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04629 Ps_contig_mira-and-tgicl-ass_franssen_34052
SEQUENCE=ACAAAAATTTCATTCATAGCCTCACACACTCTCCTTTCCCTAATCCCTCATCTTTCTCTTTTTTCTGTGTAACTTGAACGCATTACACAGAGAGAGTGACTGAGAACTGAGGGAAAATGGRGAAAATGGACACTCACTTCTCAGAACTCTCTATTATTCTCATCCACAACACTTCCAAAGCATCCTTCTTCTTCTCCACTCTTCTTTTCTAAACGTAACAACCTTTTTGGACTTCCTTTATCTTCTTCCAAACCAACTCTTCGAATTTCTTCAAGGAACAATAGGTCAATCAAATGTTCAATTTCCGAAGTCACTGAGCCGAAAACTGAGAAGAAGAAATTGATGAGAAGAGCAGATATTAGAAATATCGCAATTGTTGCTCATGTTGATCATGGAAAAACAACTCTCGTTGATGCCATGCTCAAACAAACTAAGGTGTTTCGTGATAATCAAACTGTACAAGAAAGGATAATGGACTCTAATGATTTGGAGCGTGAAAGAGGAATCACTATACTGAGTAAAAATACATCTGTTACTTATAAAGATGCCAAGATTAATATCATTGATACTCCTGGTCACTCTGATTTTGGAGGTGAAGTTGAACGTATCTTGAATATGGTCGAAGGAATCCTTCTAGTGGTAGATTCCGTCGAAGGGCCAATGCCGCAGACAAGGTTTGTCTTAAAGAAGGCTTTAGAGTTTGGTCATGCGGTTGTTGTGGTTGTGAATAAAATAGATAGGACTTCTGCTCGCCCTGATTTTGTTGTCAATTCTACCTTTGAACTCTTCATTGAGCTAAATGCAACAGATGAACAGTGTGACTTTCAGGTAATATATGCAAGTGGTATTAAAGGGAAAGCAGGGCTGTCTCCTGAAGATCTGGCAGACGACCTTGGGCCATTGTTTGAATCCATCATGAGATGTATCCCTGGACCACGTATTGATAGAGATGGCTCACTCCAAATGCTTGTTACAAGTACCGAGTATGAAGGACATAAAGGGCGTATAGCAATTGGGCGCTTGGAAGCTGGGGTTTTAGAAAAAGGAATGGAAGTCAAGATTTGCACTTCAGAAGATTCATGTAGATATGGAAGAGTTAGCGAGCTTTATGTGTACGAAAAATTCTTTAGGGTCCCTGCCGAAAGGGTGGAGGCTGGGGATATATGTGCAGTATGTGGAATTTCTGATATTCAGATTGGGGAGACAATTGCGGATAAATTGACTGGGAAGGCACTACCGTCCATTAAAGTGGAAGAACCAACAGTAAAAATGGCTTTCTCCATAAACACTTCACCTTTTGTTGGCCGTGAGGGAAAGTATGTTACCAGTAGGAATTTAAGGGACAGACTTTATCGCGAGCTTGAGAGAAATCTGGCTATGAAGGTTGAAGATGGTGAAACAGCTGATACATTCATTGTCAGCGGTCGTGGAACTCTGCATATCACCATACTCATTGAAAATATGCGAAGGGAAGGATTTGAATTCATGGTTGGGCCTCCCAAAGTTATCAACAAGAGGGTTAATGACAAAGTGTTGGAGCCGTATGAGATTGCAACTGTGGAGGTACCGGAAGTGCACATGGGAGCTGTTGTTGAACTTCTTGGCGGGAGACGAGGGCAAATGTTTGATATGCAAGGAGTTGGATCGGAGGGAACTACACTACTCAAATATAAGATCCCAACCCGTGGTCTACTTGGATTGCGTAATGCAATTTTAACTGCTTCCCGAGGGACAGCTATTCTCAACACTGTATTTGATTGTTATGGGCCTTGGGCTGGTGACATGAATACTCGGGATTTAGGCTCGCTGGTTGCTTTTGAGGGTGGAACAAGTACTTCTTATGCAATCGCTAGTTCCCAGGAGAGGGGGCATATGTTTATAGGTCCTGGAGCAGAAGTTTATAAAGGTCAAATTATTGGCATCCATCAACGCTCTGGAGACTTGTCCTTGAATGTTTGCAAGAGAAAAGCTGCAACGAATATTCGTTCCAACAAGGAACAATCAGTGATTCTTGATACACCATTGGATTATAGTTTGGATGACTGCATTGAATACATTCAAGAAGACGAACTAGTAGAGGTCACCCCCCAAAGTATAAGAATGTGCAAGAATCCAAAACTTGCAAAGAAAGGAAAGTAGACTTCCACTGTGATTCTGTGAAGCAGTAACTTGCAGATGCTTTATAAGGCAACTCTGCTAAATCTACGTAACTTCACTACCCTTTGATGCAATGTCACATATATTTGTCTGAAACTGCAGAATGATATAGGAGTTGGCGAAATGCCTTATTCATCGTAGCAACTGTTCACAGTTGATTTAGAGGATAAAATTATAGTGAATTTTGATCCATGTAGCCGATACCATTTAATGGGATAAGACTTGGTTGTTGTTGTTGAAAGTCCATATTTATCGAAAGCAAGGAACATCAAAATACGAATTGTTCA
TARGET=2383,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04630 Ps_contig_mira-and-tgicl-ass_franssen_34054
SEQUENCE=CCAACCAACTACATGACATCAATAACTCCYACTCCACTCTTYGATCTTATTTTCCTTTCCTATCACTTTGTCATTAGAATCGGCCAAGGCCATCACTACACATAATGGACGTAGTATCGAAGAAGTTATGACCATGTATGAGAAATGGTAAAACACCAAAAAGTGTATAACGGATTAGGAGAGAAAGAGCAAAGATTTCAAATATTTATGGATAACGTAAAATTCATAGATGATCACAATGATCAAAATCACACTTATAAACTTGGATTGAACCAGTTTGCGGATATGACTAATGAAGAACATAGTATGTATCTGGGCACTAGGAGTAATGATGACATTAAAAATAAGATAATCAGTCAACAGTACGCTTATAATTCCGGTGACATATTGTCGGAGTCAGTTGATTGGAGATGGGCTGTTGGTCCTATAAAAAATCAAGGAAATTGTGGTTCTTGTTGGGCGTTTTCTGCAATAGCGGCCGCGGAAGCGATTAACAAGATAGTAACAGGGAGTTTTGTGAATTTGTCTGTACAAGAGCTTGTGGACTGTGATAGAACAAACTTCAACAAAGGGTGCAGTGGTGGTATCATAGACCGTGCCTACGAATTCATCATTAATAAGTATAGTTAATAGTAATAATATATATTGAGTTGTTAATTGTTTTTCTAATGATATGATTAAAGTGCAGAAAAATACCAAGGTAGTGAGCATCAATAGTTTCAATAGTGTTTCGAGAAATAATGAAAGCTGTGGCTAATCAACCTATTAGCGTTTCTATGGATGCCTCTAGCTATGGTGTTAGATTCTATAAATCTGGTGTATTTACCGGTACATGCGAGACAAAATTGAATCATGGTGTGGTGATTGTTGGATATGGTTCTGAAAATGGTGTGGATTATTGGTTGTTGAGGAACTCATGGGGCACTAAATGGGGGTGAAGATGGTTACTTCAAGTTAGAGAGTAATATGAAAAACACAGTTAGAGGCAACTGTGGAATCGCAATGTATCCTTCATACCCAATGAAGTATGGTCCAAATTCTGCGGTTTTTACTAGGGGTAATGAAGTATGGTCCAAATTCTGCGGTCTTTACTAAGGTTAGGGATGTGCATGGATCATAAACCAAATTAAATTAAATTATATATATATGGTTCGGTTTGAATCTTAAAACCATTTCATAAAACCAATTTATTTTCTTAAAACCGACTTATGAATGGATCGGTTCGGTTTTAAACCGGTTTTTARCT
TARGET=1124,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04631 Ps_contig_mira-and-tgicl-ass_franssen_34054
SEQUENCE=CCAACCAACTACATGACATCAATAACTCCYACTCCACTCTTYGATCTTATTTTCCTTTCCTATCACTTTGTCATTAGAATCGGCCAAGGCCATCACTACACATAATGGACGTAGTATCGAAGAAGTTATGACCATGTATGAGAAATGGTAAAACACCAAAAAGTGTATAACGGATTAGGAGAGAAAGAGCAAAGATTTCAAATATTTATGGATAACGTAAAATTCATAGATGATCACAATGATCAAAATCACACTTATAAACTTGGATTGAACCAGTTTGCGGATATGACTAATGAAGAACATAGTATGTATCTGGGCACTAGGAGTAATGATGACATTAAAAATAAGATAATCAGTCAACAGTACGCTTATAATTCCGGTGACATATTGTCGGAGTCAGTTGATTGGAGATGGGCTGTTGGTCCTATAAAAAATCAAGGAAATTGTGGTTCTTGTTGGGCGTTTTCTGCAATAGCGGCCGCGGAAGCGATTAACAAGATAGTAACAGGGAGTTTTGTGAATTTGTCTGTACAAGAGCTTGTGGACTGTGATAGAACAAACTTCAACAAAGGGTGCAGTGGTGGTATCATAGACCGTGCCTACGAATTCATCATTAATAAGTATAGTTAATAGTAATAATATATATTGAGTTGTTAATTGTTTTTCTAATGATATGATTAAAGTGCAGAAAAATACCAAGGTAGTGAGCATCAATAGTTTCAATAGTGTTTCGAGAAATAATGAAAGCTGTGGCTAATCAACCTATTAGCGTTTCTATGGATGCCTCTAGCTATGGTGTTAGATTCTATAAATCTGGTGTATTTACCGGTACATGCGAGACAAAATTGAATCATGGTGTGGTGATTGTTGGATATGGTTCTGAAAATGGTGTGGATTATTGGTTGTTGAGGAACTCATGGGGCACTAAATGGGGGTGAAGATGGTTACTTCAAGTTAGAGAGTAATATGAAAAACACAGTTAGAGGCAACTGTGGAATCGCAATGTATCCTTCATACCCAATGAAGTATGGTCCAAATTCTGCGGTTTTTACTAGGGGTAATGAAGTATGGTCCAAATTCTGCGGTCTTTACTAAGGTTAGGGATGTGCATGGATCATAAACCAAATTAAATTAAATTATATATATATGGTTCGGTTTGAATCTTAAAACCATTTCATAAAACCAATTTATTTTCTTAAAACCGACTTATGAATGGATCGGTTCGGTTTTAAACCGGTTTTTARCT
TARGET=1138,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04632 Ps_contig_mira-and-tgicl-ass_franssen_34062
SEQUENCE=TTCCAGGGCTTAACACATTAGGAGTATCTTTGTCAAGAATAGACTACAAACCTAAAGGACTTAACCCGCCACACACACACCCTCGTGCCACCGAGATTGTTTTTGTGTTAGAAGGTGAATTAGATGTTGGTTTTATCACTACATCAGACAAACTCATTTCTAAGTCCATCAAAAAGGGTGAAGTCTTTGTTTTCCCAAAAGGTTTGGTGCATTATCAGAAGAACAATGGAGATAAAGCTGCTTCTGTGATTTCTGCTTTTAACAGTCAACTTCCTGGTACTTTCTCAATTGCTTCAGCACTGTTTGGTTCAACACCAACTGTTCCTGATGATGTTCTTGCCCAGGCCTTTCAGATTGATGCTAAACAGGTTGGTAGAAAATTAAGAACAAAACATGCCTACCTAAAGAAGAACTTAATATGTATATGATACTANAAGAGAGATCATTATT
TARGET=70,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0383
PRIMER_LEFT_PENALTY=0.014720
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=CCAGGGCTTAACACATTAGGAG
PRIMER_RIGHT_SEQUENCE=AGACTTCACCCTTTTTGATGGA
PRIMER_LEFT=2,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.1169
PRIMER_LEFT_1_PENALTY=0.093318
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=AAACCTAAAGGACTTAACCCGC
PRIMER_RIGHT_1_SEQUENCE=AGACTTCACCCTTTTTGATGGA
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=12.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=139
PRIMER_PAIR_PENALTY_2=0.1526
PRIMER_LEFT_2_PENALTY=0.093318
PRIMER_RIGHT_2_PENALTY=0.059316
PRIMER_LEFT_2_SEQUENCE=AAACCTAAAGGACTTAACCCGC
PRIMER_RIGHT_2_SEQUENCE=AACAGTGCTGAAGCAATTGAGA
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=303,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=60.059
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=12.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.1526
PRIMER_LEFT_3_PENALTY=0.093318
PRIMER_RIGHT_3_PENALTY=0.059316
PRIMER_LEFT_3_SEQUENCE=AAACCTAAAGGACTTAACCCGC
PRIMER_RIGHT_3_SEQUENCE=ACAGTGCTGAAGCAATTGAGAA
PRIMER_LEFT_3=47,22
PRIMER_RIGHT_3=302,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=60.059
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=12.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.1663
PRIMER_LEFT_4_PENALTY=0.014720
PRIMER_RIGHT_4_PENALTY=0.151533
PRIMER_LEFT_4_SEQUENCE=CCAGGGCTTAACACATTAGGAG
PRIMER_RIGHT_4_SEQUENCE=GACTTCACCCTTTTTGATGGAC
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=184,22
PRIMER_LEFT_4_TM=60.015
PRIMER_RIGHT_4_TM=59.848
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_04633 Ps_contig_mira-and-tgicl-ass_franssen_34063
SEQUENCE=AAGCAGTAGGTAATACAAACGCAGAGTGTGTGTGTGGCAGATTTGTACCATCAGATGTTGCAATAAAGATAAAGAAGAAACTGAACCGTAGGGTTGAAATCCTTGAAATTCAAGAATTTGAAGGCGAGGTACAAGATGAACCACCGTAATAAACAAAACAGAGAAACTTCACATTCTCAGGGTGGCTATAGTTCCTTCCTTTCCTATTACCCAATATTTCTAAAGGATATTATTTTGTATAATATAGGCGGCTAATTAATTATTCCCAAGACATCATAAATTCTTAGAAAATTTGAATGAGATATGTATATTGTTACCGGACTGAAAAAGAAAACCGTATGTTTGTAGGAAAAAAAAGTTGTGAATGCAAACTTTCTTTAAATATCATNANTT
TARGET=26,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5896
PRIMER_LEFT_PENALTY=2.460496
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=GCAGTAGGTAATACAAACGCAGA
PRIMER_RIGHT_SEQUENCE=TGTACCTCGCCTTCAAATTCTT
PRIMER_LEFT=2,23
PRIMER_RIGHT=132,22
PRIMER_LEFT_TM=58.540
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=131
PRIMER_PAIR_PENALTY_1=2.5896
PRIMER_LEFT_1_PENALTY=2.460496
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=GCAGTAGGTAATACAAACGCAGA
PRIMER_RIGHT_1_SEQUENCE=CTTGTACCTCGCCTTCAAATTC
PRIMER_LEFT_1=2,23
PRIMER_RIGHT_1=134,22
PRIMER_LEFT_1_TM=58.540
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=2.5896
PRIMER_LEFT_2_PENALTY=2.460496
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=GCAGTAGGTAATACAAACGCAGA
PRIMER_RIGHT_2_SEQUENCE=GTACCTCGCCTTCAAATTCTTG
PRIMER_LEFT_2=2,23
PRIMER_RIGHT_2=131,22
PRIMER_LEFT_2_TM=58.540
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=130
PRIMER_PAIR_PENALTY_3=2.5896
PRIMER_LEFT_3_PENALTY=2.460496
PRIMER_RIGHT_3_PENALTY=0.129103
PRIMER_LEFT_3_SEQUENCE=GCAGTAGGTAATACAAACGCAGA
PRIMER_RIGHT_3_SEQUENCE=TTGTACCTCGCCTTCAAATTCT
PRIMER_LEFT_3=2,23
PRIMER_RIGHT_3=133,22
PRIMER_LEFT_3_TM=58.540
PRIMER_RIGHT_3_TM=60.129
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=2.5937
PRIMER_LEFT_4_PENALTY=2.460496
PRIMER_RIGHT_4_PENALTY=0.133226
PRIMER_LEFT_4_SEQUENCE=GCAGTAGGTAATACAAACGCAGA
PRIMER_RIGHT_4_SEQUENCE=AAGGAAGGAACTATAGCCACCC
PRIMER_LEFT_4=2,23
PRIMER_RIGHT_4=200,22
PRIMER_LEFT_4_TM=58.540
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_04634 Ps_contig_mira-and-tgicl-ass_franssen_34073
SEQUENCE=CCGTAACGGACCCGTTCCTTTTTTCCCTTAAACCTAACCCACGAAACCTACCCCTCCCTTATCATNACCCNCTCTTTTCCTGAATTTGTACACTTCTTGCCCTTCATCCTCACTGTCATCAAAATCATCACCAACAGCATCGTCACCCATCCCGCCAAATTGCGAGAAATCCATCCCTCCCATGTCTAGGTCACCGGTACCCGCATCTTCATCTTCGTCCACCCATTTATCCCAATCTACTTTCACGTAATGTGGAGGCTTCCCTTCTGCTTTCAGTAACCTTTTCCACCACTCTTCTTCTGCTTTCTGCACAACACAGAATATGCTTCTCACCCCTACATTAATTTTGCTCTCCTCTACATTAACCTTGTCAAAGAGTGGCAAATTAAGCTCATAAAGATGGTCTCCAGCACCAGCAGTGGCAGAAAAAGTAAAAACACCATCTGGAGTAAGATCCACTTTCGCATTTTTGGAATCTGGCAACTGAACCGTGATGTAAACTTTGTCGCCCCTTTGAGCCCACTTGATCTCCGGATGACGACTCATATTTGTTTGAGAGAAGAAGAAGAAGCTGTGAAACCCTAGATTCTTTGGTAACTGACTGTCAGAGACAATTGTAACCTCGTTTTCGGATTTTGATGATTATGCAATGCAAAACCTGTTGTTTCTAGATTGTTCTTCTTTTCACTAAATTGCGTAACAGCGAAAAGT
TARGET=558,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1615
PRIMER_LEFT_PENALTY=0.039879
PRIMER_RIGHT_PENALTY=0.121635
PRIMER_LEFT_SEQUENCE=ACCTTGTCAAAGAGTGGCAAAT
PRIMER_RIGHT_SEQUENCE=CAAAATCCGAAAACGAGGTTAC
PRIMER_LEFT=364,22
PRIMER_RIGHT=637,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=59.878
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.3369
PRIMER_LEFT_1_PENALTY=0.215215
PRIMER_RIGHT_1_PENALTY=0.121635
PRIMER_LEFT_1_SEQUENCE=TTAACCTTGTCAAAGAGTGGCA
PRIMER_RIGHT_1_SEQUENCE=CAAAATCCGAAAACGAGGTTAC
PRIMER_LEFT_1=361,22
PRIMER_RIGHT_1=637,22
PRIMER_LEFT_1_TM=59.785
PRIMER_RIGHT_1_TM=59.878
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=277
PRIMER_PAIR_PENALTY_2=0.3369
PRIMER_LEFT_2_PENALTY=0.215215
PRIMER_RIGHT_2_PENALTY=0.121635
PRIMER_LEFT_2_SEQUENCE=TAACCTTGTCAAAGAGTGGCAA
PRIMER_RIGHT_2_SEQUENCE=CAAAATCCGAAAACGAGGTTAC
PRIMER_LEFT_2=362,22
PRIMER_RIGHT_2=637,22
PRIMER_LEFT_2_TM=59.785
PRIMER_RIGHT_2_TM=59.878
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.3576
PRIMER_LEFT_3_PENALTY=0.235991
PRIMER_RIGHT_3_PENALTY=0.121635
PRIMER_LEFT_3_SEQUENCE=GCTCATAAAGATGGTCTCCAGC
PRIMER_RIGHT_3_SEQUENCE=CAAAATCCGAAAACGAGGTTAC
PRIMER_LEFT_3=389,22
PRIMER_RIGHT_3=637,22
PRIMER_LEFT_3_TM=60.236
PRIMER_RIGHT_3_TM=59.878
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.4616
PRIMER_LEFT_4_PENALTY=0.340007
PRIMER_RIGHT_4_PENALTY=0.121635
PRIMER_LEFT_4_SEQUENCE=CTCCGGATGACGACTCATATTT
PRIMER_RIGHT_4_SEQUENCE=CAAAATCCGAAAACGAGGTTAC
PRIMER_LEFT_4=528,22
PRIMER_RIGHT_4=637,22
PRIMER_LEFT_4_TM=60.340
PRIMER_RIGHT_4_TM=59.878
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_04635 Ps_contig_mira-and-tgicl-ass_franssen_34087
SEQUENCE=CGGGGTTCTNGAGTTTGAACAAACTATATCCATTATTACAACTCAACACATTGTCAGCATGCAATGATTTCTTCACTCCAAATCCCCATCCAAAAACATCACAAGAGTTTCTTCTTTCACTTTCAAACGTCTCTTTCTTCTCTTCAAATTCCCTTATTTTCACCATCACATCAACACAACTAAACAAACACATAACTCTCGATCGTTGCAATCATGCTTATCAACCGGATTTCGCCGAGAAGAGCTTTGATCTACGGCCGGAGATTGTTCCGTCCTAACCCTTCCCAGAGTTTCTCTTCTGCCTCCTCCTCCTCCTCTTACTATTCCTCTGTTTCGCGATCTTTTCTATCCGCTAAATCTAACTCTCGCATAGATTTCAATTTTAAGCATGTTAAGGGATACTGTTTTTCTGCTCCTCCTGTTTTGAACATTCCCGTGGGGAACATAGTTGAAATCCCTTTGGCACAGACTGGGGAAGGTATTGCAGAATGTGAACTTCTCAAATGGTATGTTCAAGAGGGTGATTTTGTTGAAGATTTTCAACCATTGTGTGAGGTTCAAAGTGATAAAGCTACTATAGAAATCACGAGTCGGTACAAAGGAAAAGTTTGTAATGTTCTTCATGTTCCGGGTGACATTGTAAAGGTTGGAGCAACCCTTTTGAAGATATTAATTGATGAGCCTGCATGTTCTTCAATGAATTTGGGTGCCTCAGAAAATGCAAACTCATCTGATTCTGATTCTGATCAGATATCTGCCGATGAATCTGCATTTAATACAGGAGCTTTAGTCGACTCAGAAAATGTAAAGCAACTAAGTTCTGATAATGGAAAGGGGAAACAAGCTGGAGTTCTATCAACACCTGCTGTAAGGAGTCTAGCAAAGGAGCATGGTATAGATATAAATGATGTGTGTGGATCTGGAAAAGATGGAAGGGTTTTGAAAGAAGATGTGCTCAATTTTGCTGTCAACAAAGGAATCACTAAAACTCCATCTGCGGTTTTGCATGCCGATTTTGTAGAACAGCTTCATGGAGCAGAAGAACGCAGTTGTGATGCGAGAACAAAATATGATAGGCCTTCTGAGGACACGATACTCCCCCTAAGGGGATTCCAAAGAGCGATGATAAAATCAATGTCTCTGGCTGCCAAAGTCCCACATTTTCATTATGTAGACGAGATAATCTGTGATGCCCTAGTGGAGCTTAAAACGTCTTTTCAAAAGAATAATCCTTATCCAGATGTCAAGCACACTTTCCTTCCAATGCTAATCAAGTCACTATCAATGGCCCTCATCAAGTATCCCTCTGTGAACAGCTGCTTCAAAGAGGATGCATTTGAAGTCATACTCAAAGGTTCACACAACATCGGAATCGCCATGGCAACGCCACATGGTTTAGTTGTACCGAATATAAAAAATGTTCAGTCGCTTTCCATATTAGAGATAACCAAAGAGCTAGCAAGGTTGCAACAATTGGCTTCAGATAACAAGTTAACTTCTGAGGACATATCTGGTGGGACAATTACTTTGAGCAACATTGGAGCGATTGGCGGAAAATTTGGTTCCCCGCTTCTTAATCTTCCTGAAGTCTCCATTATTGCCATCGGTCGAATTCAGAAACTTCCGCGGTTTACTGAAGATGGAAATGTGTATCCCGCATCACTCATGACGGTTAATATTGGTGCAGATCATAGAGTTTTGGATGGAGCAACTGTTGCAAGATTTTGCAACGAGTGGAAAAAATTAATTGAAAATCCGGAGTTACTAATGTTGCATTTGAGATGAAATTTGATGATAAGGGACACTTAATGGAATTGTAATTTGGATGGTTTTATATTAAGCATAATAACAAAATAAATGAACTGGATCACTTAAAATTGATCAAGGTTAACGTATCATAACTTATCAAATTATTCTGATAAACAAATGCGTCATTGTAA
TARGET=302,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0280
PRIMER_LEFT_PENALTY=0.004430
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=CCTAACCCTTCCCAGAGTTTCT
PRIMER_RIGHT_SEQUENCE=GGAATGTTCAAAACAGGAGGAG
PRIMER_LEFT=273,22
PRIMER_RIGHT=433,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=0.1210
PRIMER_LEFT_1_PENALTY=0.004430
PRIMER_RIGHT_1_PENALTY=0.116582
PRIMER_LEFT_1_SEQUENCE=CCTAACCCTTCCCAGAGTTTCT
PRIMER_RIGHT_1_SEQUENCE=GAATGTTCAAAACAGGAGGAGC
PRIMER_LEFT_1=273,22
PRIMER_RIGHT_1=432,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=60.117
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.1291
PRIMER_LEFT_2_PENALTY=0.004430
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=CCTAACCCTTCCCAGAGTTTCT
PRIMER_RIGHT_2_SEQUENCE=GAACCTCACACAATGGTTGAAA
PRIMER_LEFT_2=273,22
PRIMER_RIGHT_2=558,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=0.1493
PRIMER_LEFT_3_PENALTY=0.125755
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=GTCCTAACCCTTCCCAGAGTTT
PRIMER_RIGHT_3_SEQUENCE=GGAATGTTCAAAACAGGAGGAG
PRIMER_LEFT_3=271,22
PRIMER_RIGHT_3=433,22
PRIMER_LEFT_3_TM=59.874
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=163
PRIMER_PAIR_PENALTY_4=0.2038
PRIMER_LEFT_4_PENALTY=0.125755
PRIMER_RIGHT_4_PENALTY=0.078029
PRIMER_LEFT_4_SEQUENCE=GTCCTAACCCTTCCCAGAGTTT
PRIMER_RIGHT_4_SEQUENCE=AACAGGAGGAGCAGAAAAACAG
PRIMER_LEFT_4=271,22
PRIMER_RIGHT_4=422,22
PRIMER_LEFT_4_TM=59.874
PRIMER_RIGHT_4_TM=59.922
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_04636 Ps_contig_mira-and-tgicl-ass_franssen_34089
SEQUENCE=CTACACTTGGCCACTTTTTTAAGAACTTAAAAGGAAAAAAAATAGGATAAATGAAACTACAACAACATAAAGGTTTCCTTCYACAACATAAAAGCTTCATATTAAAAGATTTCCAGAAAAATAAAACAAATCTCATAACCTTTATTTAATCTCTCAAGTTAAAACATCAAATCCTTACTCAAAATAATAATATAATTTATAAATCCAAACATAAATCAATCATGAGAAATGAGAGTACAAACCCATAGTGATTCGTTTCCATGTTGTCACTGATTGTTGTCGGATCTGGATGGTGGTCGGTAACGGAACGGAGCAGAGACATAGAGGCCTTGGGTGGCTATGGAATGATGGGTTTGAGGTGAGGTAGGAGATGATGCTTAAGGTTGCAGATCATTCGGAAAAATGGCGGAGGGAGTAGTTATGTTGAAGATGTGTGTGTTTTTGAGTTGTTGAAGGTTATGTGATGAAATATGTAAATGTTGAGGTTGAAGAAAAAAAGTCTTCTCCCTCTTTTTTTTCTTTCTGAGTATCTCTTTAGAAGTGAGTGAGTAGTGTTATATCATTTCCTTTTTCAAGAATNTGAAAGAGAGAGAGAGGAAAGTTACTTGAGAGAAAGAGATATTGTGGT
TARGET=585,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04637 Ps_contig_mira-and-tgicl-ass_franssen_34093
SEQUENCE=TCAGCCTGATGTCATTTGGGAGCAGAGAGTGAAAGACGTTGAAGCGGAACGCAATAGGCGTGTTGTAACTACCCCTGGCTTTAGTTTCTCCGCCGCTGGTCTTCTCTTTCCTTATCATCTCGGTGTTGCTCATTTTCTTATTCAGAATGGTTACATTAAGGAAACCACACCGTTAGCTGGTTCTTCTGCTGGAGCTATAGTCTGTGCTGTGATTGCCTCTGGAGCTAGTATGGAAGAAGCCTTGAGAGCTACTAAAGTATTGGCTGAAGATTGTCGAAGTCGGGGGACTGCATTTCGGCTGGGGGCTGTTCTGCGTGATATTCTTCAAGAATTTTTGCCAGATGATATTCATATAAGATCCAATGGGAGAATTCGAGTTGCTGTGACACAGCTTCTTTGGAGACCCAGGGGTTTATTGGTGGATCAGTTTGATTCTAAGGAAGATCTCATCAATGCTGTTTTTACTTCTTCCTTTATTCCAGGATATCTTGCACCAAAGCCAGCAACAATGTTTCGAAATCGATTATGCATAGATGGGGGTTTGACGTTGTTTATGCCACCGACATCTGCAGCACAAACGGTTCGTGTTTGTGCTTTTCCAGCAGGTCTAATTGGATTGGAAGGGATAGGAATTAGTCCAGACTGTAATCCCGAGAACGCGTCTAGCCCTCGTCAGCTTTTCAATTGGGCACTTGAGCCAGCAGAAGATGTAATGCTTGACCGACTCTTTGAGCTTGGATATCTTGATGCTGCTGTCTGGGCAAAGGAAAACCCAGTTGAAGAAATAGTTCAAGATGATACTCCTGCTCCTGCATTTGGAAATAGCATTGCAGAATAGCACATTATGGATTAAAGATGCACGTGGAAACAACAAATTGAATTCAAGTATGCTATGCTTGAAATGGGCATCACTTAGCAAAAAGAAATCATGGCGGGGAGGTCTACAGTTCATAACTTCTAAGTGTATACATATCATTTATTTAATATATTTATTGTAAATGGAGATAGAATTCTTGCTGTGGGCAAGAGGGGATTGAAAAGAAGAAAAAAAAAGTAATAGATATTTTATTTTATTTAAATTTACAAAATAGGGTGGTAATGTATATTGAAGTCTCAGATTTATTGTGGATTACAAATAAATAGAACTTATAGCCAGTTTCCCTATGAATAACAAAGTTAAGGCATGTGAGTTTTCTCTGAACTGATGTACTA
TARGET=1062,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2647
PRIMER_LEFT_PENALTY=0.247716
PRIMER_RIGHT_PENALTY=1.016986
PRIMER_LEFT_SEQUENCE=AGATAGAATTCTTGCTGTGGGC
PRIMER_RIGHT_SEQUENCE=TCAGAGAAAACTCACATGCCTT
PRIMER_LEFT=1002,22
PRIMER_RIGHT=1199,22
PRIMER_LEFT_TM=59.752
PRIMER_RIGHT_TM=58.983
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=1.2647
PRIMER_LEFT_1_PENALTY=0.247716
PRIMER_RIGHT_1_PENALTY=1.016986
PRIMER_LEFT_1_SEQUENCE=AGATAGAATTCTTGCTGTGGGC
PRIMER_RIGHT_1_SEQUENCE=TTCAGAGAAAACTCACATGCCT
PRIMER_LEFT_1=1002,22
PRIMER_RIGHT_1=1200,22
PRIMER_LEFT_1_TM=59.752
PRIMER_RIGHT_1_TM=58.983
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=199
PRIMER_PAIR_PENALTY_2=1.3186
PRIMER_LEFT_2_PENALTY=0.301624
PRIMER_RIGHT_2_PENALTY=1.016986
PRIMER_LEFT_2_SEQUENCE=CAAGAGGGGATTGAAAAGAAGA
PRIMER_RIGHT_2_SEQUENCE=TTCAGAGAAAACTCACATGCCT
PRIMER_LEFT_2=1023,22
PRIMER_RIGHT_2=1200,22
PRIMER_LEFT_2_TM=59.698
PRIMER_RIGHT_2_TM=58.983
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=1.3186
PRIMER_LEFT_3_PENALTY=0.301624
PRIMER_RIGHT_3_PENALTY=1.016986
PRIMER_LEFT_3_SEQUENCE=CAAGAGGGGATTGAAAAGAAGA
PRIMER_RIGHT_3_SEQUENCE=TCAGAGAAAACTCACATGCCTT
PRIMER_LEFT_3=1023,22
PRIMER_RIGHT_3=1199,22
PRIMER_LEFT_3_TM=59.698
PRIMER_RIGHT_3_TM=58.983
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=1.3816
PRIMER_LEFT_4_PENALTY=0.364634
PRIMER_RIGHT_4_PENALTY=1.016986
PRIMER_LEFT_4_SEQUENCE=CGGGGAGGTCTACAGTTCATAA
PRIMER_RIGHT_4_SEQUENCE=TTCAGAGAAAACTCACATGCCT
PRIMER_LEFT_4=932,22
PRIMER_RIGHT_4=1200,22
PRIMER_LEFT_4_TM=60.365
PRIMER_RIGHT_4_TM=58.983
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_04638 Ps_contig_mira-and-tgicl-ass_franssen_34096
SEQUENCE=CGAAATAAGTAATAATTTAGGGTCGTTTTAGTACGTCTAACTTAGTCCACCCAAAATACTCATCAAAATACAAAAGTAAACAATAGAAAAGAAAAGAAAAATAAACAAGACAACAGAAATATATCTAAACTATAAGAATAATAGCAATGCATCTTCCTTTCTGAAAAATACCAACAAATTCATCCCTAAATGCAAATCCATCTTGTGATTTTTCTCTTGTTTAAGCCACTGCAGGCATGTCCTTGAGCCAAGCATCTAGTGGTTTTCCAATGGAATAAACAATGAAACCAATTTCCCTCAGCTTTTTAACATCCACAACATTCCTTCCATCAAAGATAAATGCAGGCTTCTGCATGTTGTCGAAAACTCTTTGGTAATCAAGGTTCTTGAACTCGTCCCATTCGGTCATAATGCAAATACCATGTGAGTCCTTCAACGCCTCATACGCGTCCCAAACCACAGAAACTTGTTTGTTGGAAGTTGTTGGGCTTGTTGGTTGAAGATGAGCAGGGTGATCCCAATCAAATTTCTTCATTGCCAAATCCTTCAAGATTTGCTCTTCAGAAACTTGAGGATCATATATGCTCAATTTAGCCTTGTCTCCCAATAAACCCTTGCACACATCGATCGCAGGAGTTTCCCTAGTGTCGCCGGTGTCCTTCTTGAAAGCAAATCCCAAAACAGCAATCTTTTTTCCTGACACCGTGTTGAACATGGAAGAAACAACCCGGTTCACGAATCGAGATTTCTGGTAGTCATTCACCTTAATGACCTGTTTCCAGTAGTTAGCAACCTCGGGAAGGCCATTGCACTCGCAGATATAGACCAAGTTCAGAATATCCTTCTGAAAGCACGATCCACCAAAACCAACACTAGCATTCAAGAACTTCGGTCCAACTCTCGAATCAGTTCCGATCGAGTGAGAAACCTGAGCAACATCAGCACCGGTTGCCTCGCATAGCGCGGACATGGCGTTCACAGAGGAGATTCTCTGTGCCAAAAAAGCATTGGCGGCAAGCTTCGAGAGTTCAGCAGACCACAAATTGGTGCAAAGGATTCGATCCAAAGGGACCCAATGAGCATAAACATCTCTCAAAGTATGGATAGCCTTTTGACCTTCAGGAGTTTCCCTACCTCCGATAAGAACACGATCCGGATTATACAAATCTTTGATAGCTGTACCCTCTGCAAGAAACTCAGGGTTAGACAAAATTGTGAAATTAATACCCTTCCTATTGTGAGTCAAAATCCTCTCAATTGCCTCGGCTGTCTTAACCGGAACAGTYGACTTCTCAACAACAATTTTATCAGATTTTGAAACATCAGCAATCATCCTAGCAGCACTCTCCCAGTAAGTAAGATCAGCTGCTTTGCCAGCTCCAAGACCTTGAGTCTTTGTAGGAGTATTCACCGAAACAAAGACGATATCAGCCTCAGCAACATGCTTCTCAACATCAGTGCTGAAGAAAAGGTTCCTTCCTCTGCACTCCTTCACCACATCATCAAGACCAGGCTCATATATTGGTAGATGCTCACTGTTCCAAGCATTGATTCTCGGCGCCGCAATGTCCACCACAGCTACCTCAATCTCAGGACACTTCAAAGCAATCACAGCCATTGTTGGACCACCAACATAGCCAGCTCCAATACAGCAAATCTTCACCATTTTTTCTTAGCAACCTTAGTTAAGGAGAGGTTGAGAACGAAAGGAGGAAGAGAGATCTGAGGGGAAGGAGTAGAAGAAATCAGAGGAT
TARGET=85,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04639 Ps_contig_mira-and-tgicl-ass_franssen_34097
SEQUENCE=ATAGAAAGAAAGAATACCATAGAAATAAAGTAAGTGGGATTGAATTATCTTGTCCTATTGTTTCATCAAACAAGTGTCAAGTGATAATAACAAGATAGCATAGTTTATCCAATCATACCTCTCCTATCTACATATATAACCATGTCCACATTACAAGAATCCTTGTGGACATTGATCATTACAACCATGCTCTCAAGTACATCATTTCTCACTGCCATAGTTCATATCATTTGGAATATTTGCATCACTATTCATCTGGACTATATACATTTGATCCTTTAGAGTCACTTCTCAGAGAGCATCTTGTCAATTTGACCATTTTGGTTGTACAGATCCACAATTTTCTTAGCATATGTAGGCATGTGTCTATCAGCCCTTTCCTTTCCCCACAACCGATTTACGTATAAATAGTTCCGAATGGTATGATAATCAAGCGAATAACGTGCAAATTCAATACCCTTTGGACCAATCAAATTTAGTATAAATGCAAGCAAGTTTCCAACAAACTTTGGAGCAGGCTGAGAAGGACCTTTTCCGAGTTTAGCTTTATCATCGGCCTTAACAGTCTCCATGACAAATGGTCGCCTATCCCCGGAGTTAGTTGTTGGAGTAATCTCGAGTAAGTTCTCTATAAGACTCAACATCTCTCTTCCTCGTTCGTTCCTGACAGTAATATATTGAGGATGTTGTGTCATGCTGATTCCGGAGTATTTCGGCACGCCCATATATCCAACAACCAAGTCTGCTAAAGCATTTGTATAGTCAAAACAGCTGTAGCAAGATGGAGCAATAACGTCAACTAAATCATTTGCTGGTAGACAGAAATAAGGAACCTCCTCGATACGGCCGTCCAAATGCTTCAAGTGAACCTTGTAATCTTGCATAAACTCATAATGGAGAACTGTTTCAGGTGAATCACTCGCAGCGTTTAGAAACTTATCCAGTCCTTCTCTAGGTCCATTATCCACACAGTTAGTGCCTAAAACATAGAGTTTATCCAAATTCAAGTGGTGCTCGACAGATCTTAATGCTTGCACCTGGCAACCTACACCACAGAAAAGAAGACGCTTGACACCTGCAGCCTCAACAAGTGCAAGGGTATTAAGGTTAGGAGATAATGTTGGTTTGACACCTCTAGCAGCCAACACTTCTTCCGGTGTCCTTGCTAAAACAGGTCTTGGAGCAAATCTGTCATCAGGATCACTTTGTACGCAAATGACAGCCTCAACCATGCCTGATTTAAGCATCTCAATTGCAATGGTTGTAACTATACCAGTCCATTGAGCTCCTTCAACAGGGATAAGTTTCCGAGCATATAGAAGTTCCTCATGGACTCCTAAGTAGGTTTCGTCTAATGTATCTGTTTTTCTCCCTCTACCATGAACAACAGGTTCCAATTTTTCAATCCTAGACATTCCATCTCCCAAGAAAGCACAAGCATTCTTAACATGAGCAATATAATATGTATCACACAAACCGCAACGACTGCATTGATCTTTAGCTGGGTAGGTTCCACCTGGTGGGATAGGTTTGGACCTTTTTCTCCAGTCCTGTTTTACAGTACCATCTTGAGATGAAGAAGAACGAATGGAGAGAGAAAATGGTAGAGAGGTGAGTTTGGGAACGAAGGAAGACATGGCTTAGCAGTGAGTGAAAACTGATGTGGCTTTTTCTGTTCCACCCCCG
TARGET=3,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04640 Ps_contig_mira-and-tgicl-ass_franssen_34102
SEQUENCE=CAAACTGCAAAAACAGATTAAAACTCTTTCTTTCCGTTAAACTCNNNGCTTTCTTGTTGTTGTTGTTGTTGTTGTGAAACGCAACAGAATATTGTTATTGTTGTTCTGCTATCAATGGAGTTCGATCCGATCCCAATCGGTTCGTGCTCCAAAGATCACCACAACATTTATCAAAACTGGTTCAATCATGCTGATTCAGATGCTGATGGCCGCATAACGGGGAATGACGCCACCAAGTTTTTCGCCATGTCTAATTTGTCTCGTCAAGATCTTAAGCAGGTGTGGGCTATTGCAGATTCAAAGCGACAAGGTTATCTAGGTTTCCAAGAGTTTATAGTTGCTATGCAGCTAGTTTCTTTGGGACAATCTGGGCACCCAATAACACATGATCTGCTGAGTAGTGATGTTGATTTGAAAAATTTGAAACCTCCCATAATGGAGGGTTTGGATGTATTACTAGCTAAGAAAAAGCATAAACAAAAAGAAGTGGATGTAAATGGTAGTTCTCAATTACAGCCATCACTATCAAGTAGTTGGTTTTCTTCAAAATCCACAAAAAAGATTCCACTTTCTTCTGTGACATCAATAATTGATGGCCTGAAGAGACTCTACATCCAAAAGTTGAAGCCTTTAGAAGTTACTTATCGCTTCAATGATTTTGTCTCCCCGTTACTGACAAATAGTGATTTTGATGCCAAACCTATGGTTATGCTTTTGGGTCAATACTCAACTGGTAAAACAACATTTATCAAACATCTGCTTAAATGTAGTTATCCAGGTGCACATATTGGACCCGAGCCTACTACTGATAGGTTTGTTGTTGTCATGTCTGGACCTGATGAGAGAAGCATTCCTGGAAATACTGTTGCTGTCCAAGCAGACATGCCATTTAGTGGACTCACAACTTTTGGCACAGCTTTTTTGTCAAAATTTGGGTGTTCACAAATGCCTCATCCTCTTCTGGAGCACATTACGTTTGTGGACAGTCCCGGAGTTCTATCTGGARAAAAACACGAACGCAAAGAGCCATATGTAAGTTTTACGGGATAAACCATTCCGTTTTGGGGTTTTACGAC
TARGET=54,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04641 Ps_contig_mira-and-tgicl-ass_franssen_34110
SEQUENCE=ATTATTATTATTATCTTACGACAATCTCTATACCTTATGACCGAACCACCTTCCTTGTAAATCNAACCATTTCATTTTCTTTTCTTCTTCTTGTTCTATCTTTTTTGCTCATCTTTTCTTGTCCTTTCTCTCATTTTTTCCCTATATATCTTCCACTCTTTCTTTTTTTTTCTTCCTATAATCAAAGATCATGGAAGACTCTCCTGCTGCTTATATCCACCTGGTGCACCGTTTGATAGAAGAGTGTATCTTATTCAACATGGAGACCAAAGAAGAATGCAGTGGGAGGCCTTCCTTTACCCAAAACNTGACAA
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04642 Ps_contig_mira-and-tgicl-ass_franssen_34122
SEQUENCE=CTGATAACAGTGCATTCAGTCTGCCCGAATCTAAACTTGATAGATATTCAGAGCAGACATCTGCAGGTAAAGAATTGCCTGAGTTCCTCAAGCAGAAGTTAAGAGCTCGGGGTATTCTTAAAGAATGATATACATACCAAAGATATTAAAGGTACTTCAGCTGAACTTAGGGGAAATGAAAACTTGCCTTCAGGATGGGTGGAAGCAAAAGATCCGGGGAGTGGTGCATTTTACTATTATAATGAAAGTACCGGGAAGAGTCAATGGGCAAAACCTTGCGAAGCATCCTTAACTGAGCAATCAACACCGTCTTTGAATCTTCCGGAAAATTGGGTGGAAGCATTGGACGAAACATCAGGTCACAAGTATTACTATAATACAAAAACTCACGTATCACAATGGAAGCATCCAAATTCTTGCTCAGGAAATTAGTGTCAGATAAATTGGCCTTGTCAGTTGGTCACGTAGATGACCAATCATCTAATTTAAAGAGGTGCATGGGCTGTGGTGGTTGGGGTGTAGGCCTTGTGCAAGCATGGGGTTCATGCGATCACTGCACGAGTGTTTTCAATCTTCCACAATGTCAGTATTTGTCCTCAAGTTTGAATAATCAGCAATGCAGTGCTGCACATCCCACTGAAAGTTCTGATAAAACTGCTACCAAGCAGAGGGCCAGTTGGAAACCTCCATTTGATAAAGGAAATAAAAAAATGAGCAAGAAGCGCGCTCATGAAGAGGATGATGAGTTGGATCCTATGGACCCCAGCTCCTATTCAGATGCTCCTCGTGGTGGCTGGGTGGTGGGTCTGAAAGGTGTGCAGCCAAGAGCAGCTGACACAACAGCAACTGGTCCTCTGTTTCAACAACGACCATATCCATCACCCGGAGCTGTTTTACGAAAGAATGCTGAAATTGCCTCCCAGAAAAAGAAACCAAATTCAAATTTTATTGCCATATCCAAGAGAGGCGATGGGAGTGATGGGCTTGGTGATGCAGACTAATACCATCTTTATCACCTTTGTCGTGTTATTTGTAAGTTAGCTAAGGATGCATTTTGTTGTGAAAACCAGCTTTGCTGCTGTGCAATAATATTCTGTGGTTTTTACCGGAAAGTATCGATTTGATGCACGACTGGACAGAAGATATCTCTGTAACACATAACTGGTGTTGATAAACTTGAAAGCCTTCTGGCAAGTGATGGTAAAACTTGCACTTAGCTTGGTAATTCACGAGCTGACCTGAGAAGTTCAAGAGGACCTCAAGATTGGGAAAGAAAGCACCTTTCAGAATTTTCAATGCTCTTTTCTTTTTGTTAATCTTTGGATTTTCCTGTTAATCATATTTATTCAAGAAATATATTATCATGTAGATGCAGTCCTGGCCTTTTTTGCCTTCTTGACATAACTCTCTGGGAATTTGGGGGAGTCTTTTGGTTATGGATCTTGAGGGATTTCATTATTTTTATTATTATTATGTGTTATGTCTCTTATTTCAAGAACATATAGATGTTTATATCTAGTTAACATCGTAAG
TARGET=1462,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04643 Ps_contig_mira-and-tgicl-ass_franssen_34134
SEQUENCE=CCCTTGTTCAAACAAATTGTAAATCAATTTGTACATGATCGCTCACGTCCAGAGGCTATCACTGTTGGAATAAATGCAGTAAGGGAGATGTGCCTGCGTATACCACTGTTAATGAGTGAAGATTTACTACAAGACCTTGCCCTCTATAAGAAGTCACATGAGAAGGGAGTTTCAATAGCAGCTCGATCTTTGATTACATTATTCCGAGAGATTTGCCCTTCACTACTAATTAAGAAGGACCGAGGGCGACCTACTGACCCCAAGGCTAGACCAAAGGCTTATGGAGAAGTCAATATTGCGACTGACGTTCCTGGTGCTGAACTGTTGCAGAATAGTGACGATGATGTTGAGCAGGATGGTAATCATTCTGATGATTCTGCACATAGTGACTCAGACGATGACCAGGAAGATGATCAGGTAAGTTTAAATCTCAACGATGAGAACCAGCTAGGCAGTGATAACACCGGAAGTGATGATGATGAAGACAAAGATCCTGATGTTGTATCAGAAGATGAAAACGATGGAAGTTCTGATTATGAAACCAATGACGATGTTGAAGATGAGGATGATGATGATCTAGAAGACAGTGAAGAGGATGATGAAGAGGATGGTGAGATTTCAAATCATGAAGGAGATGGTGATCTGCATACAATTCATGATGGAAGTATGGATACAAAAACTACATTAAATGACTCGACAAAAAAGAGGAAGTTTAGAGATTTCAATGATCAACTTATATCTGCTGATACAAGTCTCCGGGCTTTGAAGAAATTGGCAGGAACAACAATAGAGAATTCACTGCCAGAAAAGGAAGATGGAATTCTTTCTAATGAAGACTTCCAGAGGATCAAGGAACTAAAGGCAAGAAAAGAAGCAAGAACTGCATTAGCGCAACATGGGCTTGTAAAGTCATCAACAAATAAGATTCCAAGCTCTGATCAACTTAGTTTAAAGCGAGTTGATGGTTCCATGCTTGAAGCTCATGTAAAGAAAAAGCTTAACAAGGAGGAGAGGTTGGCAATGGTTAGAGCAGGGAGGGAGGAAAGAGGACAGTACCATTCTCGAGCAGCTGTAAAACAGAAAAAGACGGGTGGTCTTAGTAACAGACAAAAGGAACACAAGAAAAGCATGCCTCTAATTGCGAAGAGGAACAAGATAGCAAGAGTTAAGCTAGAGAAAAAGATTAAACGGCAGAGGTCTGGCAAACAATTCCGGGGGAGGAAAGCGTGGCAATAGGAGGCAACAAATATGAATCTGCATTATAAACTTATTTTTTTTGTTATTTTTGAATGTCATTTTTACTTATTGCTTGAATTTTGTTATATTAAATTTTGACGGTGTAATATTTATAGAAAATCCTTGGATTGAATATTGACTATGCATACCAGAGGATCAAATTTTATTTTCCTAGTAAGTTGGCATAAATTAAAGGATAGTTTCTTGTACCAAACTG
TARGET=471,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1035
PRIMER_LEFT_PENALTY=0.036112
PRIMER_RIGHT_PENALTY=0.067436
PRIMER_LEFT_SEQUENCE=AGACGATGACCAGGAAGATGAT
PRIMER_RIGHT_SEQUENCE=CACCATCTCCTTCATGATTTGA
PRIMER_LEFT=392,22
PRIMER_RIGHT=639,22
PRIMER_LEFT_TM=59.964
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.1035
PRIMER_LEFT_1_PENALTY=0.036112
PRIMER_RIGHT_1_PENALTY=0.067436
PRIMER_LEFT_1_SEQUENCE=AGACGATGACCAGGAAGATGAT
PRIMER_RIGHT_1_SEQUENCE=TCACCATCTCCTTCATGATTTG
PRIMER_LEFT_1=392,22
PRIMER_RIGHT_1=640,22
PRIMER_LEFT_1_TM=59.964
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=0.4537
PRIMER_LEFT_2_PENALTY=0.312895
PRIMER_RIGHT_2_PENALTY=0.140832
PRIMER_LEFT_2_SEQUENCE=GGTGCTGAACTGTTGCAGAATA
PRIMER_RIGHT_2_SEQUENCE=CATCTTCAACATCGTCATTGGT
PRIMER_LEFT_2=312,22
PRIMER_RIGHT_2=561,22
PRIMER_LEFT_2_TM=60.313
PRIMER_RIGHT_2_TM=59.859
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.4981
PRIMER_LEFT_3_PENALTY=0.357294
PRIMER_RIGHT_3_PENALTY=0.140832
PRIMER_LEFT_3_SEQUENCE=GTCAATATTGCGACTGACGTTC
PRIMER_RIGHT_3_SEQUENCE=CATCTTCAACATCGTCATTGGT
PRIMER_LEFT_3=288,22
PRIMER_RIGHT_3=561,22
PRIMER_LEFT_3_TM=59.643
PRIMER_RIGHT_3_TM=59.859
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.5173
PRIMER_LEFT_4_PENALTY=0.449829
PRIMER_RIGHT_4_PENALTY=0.067436
PRIMER_LEFT_4_SEQUENCE=TGACCAGGAAGATGATCAGGTA
PRIMER_RIGHT_4_SEQUENCE=CACCATCTCCTTCATGATTTGA
PRIMER_LEFT_4=398,22
PRIMER_RIGHT_4=639,22
PRIMER_LEFT_4_TM=59.550
PRIMER_RIGHT_4_TM=59.933
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_04644 Ps_contig_mira-and-tgicl-ass_franssen_34134
SEQUENCE=CCCTTGTTCAAACAAATTGTAAATCAATTTGTACATGATCGCTCACGTCCAGAGGCTATCACTGTTGGAATAAATGCAGTAAGGGAGATGTGCCTGCGTATACCACTGTTAATGAGTGAAGATTTACTACAAGACCTTGCCCTCTATAAGAAGTCACATGAGAAGGGAGTTTCAATAGCAGCTCGATCTTTGATTACATTATTCCGAGAGATTTGCCCTTCACTACTAATTAAGAAGGACCGAGGGCGACCTACTGACCCCAAGGCTAGACCAAAGGCTTATGGAGAAGTCAATATTGCGACTGACGTTCCTGGTGCTGAACTGTTGCAGAATAGTGACGATGATGTTGAGCAGGATGGTAATCATTCTGATGATTCTGCACATAGTGACTCAGACGATGACCAGGAAGATGATCAGGTAAGTTTAAATCTCAACGATGAGAACCAGCTAGGCAGTGATAACACCGGAAGTGATGATGATGAAGACAAAGATCCTGATGTTGTATCAGAAGATGAAAACGATGGAAGTTCTGATTATGAAACCAATGACGATGTTGAAGATGAGGATGATGATGATCTAGAAGACAGTGAAGAGGATGATGAAGAGGATGGTGAGATTTCAAATCATGAAGGAGATGGTGATCTGCATACAATTCATGATGGAAGTATGGATACAAAAACTACATTAAATGACTCGACAAAAAAGAGGAAGTTTAGAGATTTCAATGATCAACTTATATCTGCTGATACAAGTCTCCGGGCTTTGAAGAAATTGGCAGGAACAACAATAGAGAATTCACTGCCAGAAAAGGAAGATGGAATTCTTTCTAATGAAGACTTCCAGAGGATCAAGGAACTAAAGGCAAGAAAAGAAGCAAGAACTGCATTAGCGCAACATGGGCTTGTAAAGTCATCAACAAATAAGATTCCAAGCTCTGATCAACTTAGTTTAAAGCGAGTTGATGGTTCCATGCTTGAAGCTCATGTAAAGAAAAAGCTTAACAAGGAGGAGAGGTTGGCAATGGTTAGAGCAGGGAGGGAGGAAAGAGGACAGTACCATTCTCGAGCAGCTGTAAAACAGAAAAAGACGGGTGGTCTTAGTAACAGACAAAAGGAACACAAGAAAAGCATGCCTCTAATTGCGAAGAGGAACAAGATAGCAAGAGTTAAGCTAGAGAAAAAGATTAAACGGCAGAGGTCTGGCAAACAATTCCGGGGGAGGAAAGCGTGGCAATAGGAGGCAACAAATATGAATCTGCATTATAAACTTATTTTTTTTGTTATTTTTGAATGTCATTTTTACTTATTGCTTGAATTTTGTTATATTAAATTTTGACGGTGTAATATTTATAGAAAATCCTTGGATTGAATATTGACTATGCATACCAGAGGATCAAATTTTATTTTCCTAGTAAGTTGGCATAAATTAAAGGATAGTTTCTTGTACCAAACTG
TARGET=565,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1035
PRIMER_LEFT_PENALTY=0.036112
PRIMER_RIGHT_PENALTY=0.067436
PRIMER_LEFT_SEQUENCE=AGACGATGACCAGGAAGATGAT
PRIMER_RIGHT_SEQUENCE=CACCATCTCCTTCATGATTTGA
PRIMER_LEFT=392,22
PRIMER_RIGHT=639,22
PRIMER_LEFT_TM=59.964
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.1035
PRIMER_LEFT_1_PENALTY=0.036112
PRIMER_RIGHT_1_PENALTY=0.067436
PRIMER_LEFT_1_SEQUENCE=AGACGATGACCAGGAAGATGAT
PRIMER_RIGHT_1_SEQUENCE=TCACCATCTCCTTCATGATTTG
PRIMER_LEFT_1=392,22
PRIMER_RIGHT_1=640,22
PRIMER_LEFT_1_TM=59.964
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=0.3824
PRIMER_LEFT_2_PENALTY=0.140832
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=ACCAATGACGATGTTGAAGATG
PRIMER_RIGHT_2_SEQUENCE=TCTTCCTTTTCTGGCAGTGAAT
PRIMER_LEFT_2=540,22
PRIMER_RIGHT_2=814,22
PRIMER_LEFT_2_TM=59.859
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.3943
PRIMER_LEFT_3_PENALTY=0.140832
PRIMER_RIGHT_3_PENALTY=0.253482
PRIMER_LEFT_3_SEQUENCE=ACCAATGACGATGTTGAAGATG
PRIMER_RIGHT_3_SEQUENCE=TCTCTATTGTTGTTCCTGCCAA
PRIMER_LEFT_3=540,22
PRIMER_RIGHT_3=792,22
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=59.747
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=253
PRIMER_PAIR_PENALTY_4=0.3943
PRIMER_LEFT_4_PENALTY=0.140832
PRIMER_RIGHT_4_PENALTY=0.253482
PRIMER_LEFT_4_SEQUENCE=ACCAATGACGATGTTGAAGATG
PRIMER_RIGHT_4_SEQUENCE=TTCTCTATTGTTGTTCCTGCCA
PRIMER_LEFT_4=540,22
PRIMER_RIGHT_4=793,22
PRIMER_LEFT_4_TM=59.859
PRIMER_RIGHT_4_TM=59.747
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_04645 Ps_contig_mira-and-tgicl-ass_franssen_34143
SEQUENCE=ACGAATTCGCCAATTTCACGGCGACGAAAGCGAGTGACCTCGCGCAACCGTTGGTGGAGTCACAAACTCCGTTTAAGATCCACATTGTGAAGGATCATGATATGAAGGAGAGGTTGTGTTTGGAAGTTGAGAGGCTTGGGTTGAGTGCTGTGATCATGGGGAGCCGTGGATTCGGTGCTTCGAAGAGTGCTTCTACGGGGAGACTTGGAAGTGTGAGTGATTATTGTGTTCGTCACTGTGTTTGTCCTGTTGTTGTTGTTCGTTATCCTGAAGAGAGTAATGGCGGTGGAGTTCAGGGGAAAGATAGTGTTGGTGTTGGTGAAAAAGTGGAACTTCATCCTGTGCCTGAGGAAGAACTTGAGGAATATCATGACGCCCCTGATGAACAGAAAGATGCTTAAAGAGGTTACAGAGAAATATCAATACCCTACTAACTTCAGAAAATGGTTATAGCATCAAGACATGCGTCAGATGGAGAAGAATATGTTAAACTTATTTGAAAAAATTATACACTATAAATCGATTGTTTGGTTGGTTGGAATTTGCATGCGTTTGAACNGTTTGTTTTTATACTGCTTCAATAGTGACGGTTCGAATACACCATCTTTCTATTTCGATGATTGGATTCAAAGACTCTTGATCCACTTCATGTTAGAACCAGTGTAGATTTAGAANAAACTTGTTTCTGTCTTCTGTTC
TARGET=248,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3412
PRIMER_LEFT_PENALTY=0.205226
PRIMER_RIGHT_PENALTY=0.136017
PRIMER_LEFT_SEQUENCE=GTGTTTGGAAGTTGAGAGGCTT
PRIMER_RIGHT_SEQUENCE=ACCAACACTATCTTTCCCCTGA
PRIMER_LEFT=115,22
PRIMER_RIGHT=313,22
PRIMER_LEFT_TM=59.795
PRIMER_RIGHT_TM=59.864
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.4812
PRIMER_LEFT_1_PENALTY=0.345221
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=AAGGAGAGGTTGTGTTTGGAAG
PRIMER_RIGHT_1_SEQUENCE=ACCAACACTATCTTTCCCCTGA
PRIMER_LEFT_1=104,22
PRIMER_RIGHT_1=313,22
PRIMER_LEFT_1_TM=59.655
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.5498
PRIMER_LEFT_2_PENALTY=0.205226
PRIMER_RIGHT_2_PENALTY=0.344527
PRIMER_LEFT_2_SEQUENCE=GTGTTTGGAAGTTGAGAGGCTT
PRIMER_RIGHT_2_SEQUENCE=CCAACACTATCTTTCCCCTGAA
PRIMER_LEFT_2=115,22
PRIMER_RIGHT_2=312,22
PRIMER_LEFT_2_TM=59.795
PRIMER_RIGHT_2_TM=60.345
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=0.5532
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.347935
PRIMER_LEFT_3_SEQUENCE=GTGTTTGGAAGTTGAGAGGCTT
PRIMER_RIGHT_3_SEQUENCE=GGCGTCATGATATTCCTCAAGT
PRIMER_LEFT_3=115,22
PRIMER_RIGHT_3=376,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=60.348
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.6083
PRIMER_LEFT_4_PENALTY=0.308566
PRIMER_RIGHT_4_PENALTY=0.299723
PRIMER_LEFT_4_SEQUENCE=TGTGTTCGTCACTGTGTTTGTC
PRIMER_RIGHT_4_SEQUENCE=CTGACGCATGTCTTGATGCTAT
PRIMER_LEFT_4=224,22
PRIMER_RIGHT_4=470,22
PRIMER_LEFT_4_TM=59.691
PRIMER_RIGHT_4_TM=60.300
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_04646 Ps_contig_mira-and-tgicl-ass_franssen_34143
SEQUENCE=ACGAATTCGCCAATTTCACGGCGACGAAAGCGAGTGACCTCGCGCAACCGTTGGTGGAGTCACAAACTCCGTTTAAGATCCACATTGTGAAGGATCATGATATGAAGGAGAGGTTGTGTTTGGAAGTTGAGAGGCTTGGGTTGAGTGCTGTGATCATGGGGAGCCGTGGATTCGGTGCTTCGAAGAGTGCTTCTACGGGGAGACTTGGAAGTGTGAGTGATTATTGTGTTCGTCACTGTGTTTGTCCTGTTGTTGTTGTTCGTTATCCTGAAGAGAGTAATGGCGGTGGAGTTCAGGGGAAAGATAGTGTTGGTGTTGGTGAAAAAGTGGAACTTCATCCTGTGCCTGAGGAAGAACTTGAGGAATATCATGACGCCCCTGATGAACAGAAAGATGCTTAAAGAGGTTACAGAGAAATATCAATACCCTACTAACTTCAGAAAATGGTTATAGCATCAAGACATGCGTCAGATGGAGAAGAATATGTTAAACTTATTTGAAAAAATTATACACTATAAATCGATTGTTTGGTTGGTTGGAATTTGCATGCGTTTGAACNGTTTGTTTTTATACTGCTTCAATAGTGACGGTTCGAATACACCATCTTTCTATTTCGATGATTGGATTCAAAGACTCTTGATCCACTTCATGTTAGAACCAGTGTAGATTTAGAANAAACTTGTTTCTGTCTTCTGTTC
TARGET=528,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5318
PRIMER_LEFT_PENALTY=0.299723
PRIMER_RIGHT_PENALTY=0.232060
PRIMER_LEFT_SEQUENCE=ATAGCATCAAGACATGCGTCAG
PRIMER_RIGHT_SEQUENCE=AAAGATGGTGTATTCGAACCGT
PRIMER_LEFT=449,22
PRIMER_RIGHT=607,22
PRIMER_LEFT_TM=60.300
PRIMER_RIGHT_TM=59.768
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=159
PRIMER_PAIR_PENALTY_1=0.5488
PRIMER_LEFT_1_PENALTY=0.299723
PRIMER_RIGHT_1_PENALTY=0.249074
PRIMER_LEFT_1_SEQUENCE=ATAGCATCAAGACATGCGTCAG
PRIMER_RIGHT_1_SEQUENCE=AAGATGGTGTATTCGAACCGTC
PRIMER_LEFT_1=449,22
PRIMER_RIGHT_1=606,22
PRIMER_LEFT_1_TM=60.300
PRIMER_RIGHT_1_TM=60.249
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=0.5800
PRIMER_LEFT_2_PENALTY=0.347935
PRIMER_RIGHT_2_PENALTY=0.232060
PRIMER_LEFT_2_SEQUENCE=ACTTGAGGAATATCATGACGCC
PRIMER_RIGHT_2_SEQUENCE=AAAGATGGTGTATTCGAACCGT
PRIMER_LEFT_2=355,22
PRIMER_RIGHT_2=607,22
PRIMER_LEFT_2_TM=60.348
PRIMER_RIGHT_2_TM=59.768
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.5963
PRIMER_LEFT_3_PENALTY=0.364233
PRIMER_RIGHT_3_PENALTY=0.232060
PRIMER_LEFT_3_SEQUENCE=GGTTATAGCATCAAGACATGCG
PRIMER_RIGHT_3_SEQUENCE=AAAGATGGTGTATTCGAACCGT
PRIMER_LEFT_3=445,22
PRIMER_RIGHT_3=607,22
PRIMER_LEFT_3_TM=59.636
PRIMER_RIGHT_3_TM=59.768
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.1000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=163
PRIMER_PAIR_PENALTY_4=0.5970
PRIMER_LEFT_4_PENALTY=0.347935
PRIMER_RIGHT_4_PENALTY=0.249074
PRIMER_LEFT_4_SEQUENCE=ACTTGAGGAATATCATGACGCC
PRIMER_RIGHT_4_SEQUENCE=AAGATGGTGTATTCGAACCGTC
PRIMER_LEFT_4=355,22
PRIMER_RIGHT_4=606,22
PRIMER_LEFT_4_TM=60.348
PRIMER_RIGHT_4_TM=60.249
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=11.1000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_04647 Ps_contig_mira-and-tgicl-ass_franssen_34144
SEQUENCE=AAGACAGTGGTTATCAACGCAGAGTACRGNGGGGATTGGTATAAAAAAAGTTACTAGTACAAGAAGAAGAAGAAGGGTGCGCAATACGCACATAAAATCGAAGGTTTATTCAGTGTGCAAATTAGCCACCAAAAACGCAATTCTCGTTTGGGTCAAGTGAAGTCTTTGCGAACAATTCACAAAAATGGCATCCAAATCACAGAAGAAAGCCAATCTCTTAGATCATCACTCCATTAAGCACATTCTCGATGAGTCTGTTTCTGAGGTCGTTAAGGGACGTGGGTATGTTGAAGATGTGAGATTGAGCAATGTGAGGTTGTTGATTGGGTCTGTTATTATTGTCATTGCTCTGTTTGCTCAGTTTTACAAGAAGAAGTTTCCTGAGAATAGGGATTTTCTTCTTGCCTGCATCGCCTTATATGTAATCTTCAACGGGTTGTTGCAGCTGATCATATACACTAAGGAGAAGAATGCCATTCTCTTTACTTATCCTCTGTCTGGCTCCTTTACCAGCACTGGTTTGGTAGTTTCCTCCAAATTACCCAGATTTTCTGATATCTACACACTTACTGTAGAAAGTGCAGATCCAAAATCAATTTCTGCAAATGAACCAGTAAATCTTACCAAAAGTGTTACTGAGTGGTTCACTAAGGATGGAGTCTTAGTTGAAGGCCTCCTCTGGAAGGATGTTGAAACTCTGATAGCTCAATATACTAAAGAACCAAAGAAGAGCAAGTGATATCGAGAAATGAACTTGGTTGTCCAAAGAGCTTTTCTGAGGACAAATATGTATTTGGATATGGTTTACATTTCTATACATTTTCAACATTTAGAACAAAATTGAGCAATTTAAGCGTTCAGTAACTTTAGAACACACTTAATTCCCATTTTTCATCGTTTATTTTATAGGAAAAGGTTCCTAAAACCATTTAATGGTTATCATTATTCTTTTATTTTACG
TARGET=61,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04648 Ps_contig_mira-and-tgicl-ass_franssen_34145
SEQUENCE=ATTCTCAAACAATCTCATGTCAGTAGATAAACTACAGATAAGTCAGTACAGACAGGTATATAGTTTGTGTTTCCCTTCACAATATTTGCAACATGTCATAGTATGTAGAACAAGATATTGTTTATCGCAATGGAATCAATCAACTATCCAACATCACTCAAATTACAGTTAATTTAAAAATGTTAACACTCCCCTCAAGGAATTGAGTAACAATACAGGCGTTCCTCTTGGGCTGCCATTGGCTATTTGAATATTCCAAGATATTCGGCGAGTAATGGCTGTTCAAGTAAATCAATCAATAGAATATTCAATAACAGTAGCGAGAGAAACAGAAGGGGATGTTTTAACTGTCAAAGTACACACTATACACATCTTGACAGGTTCCTGAATCTTTGTTTCGTTTCACTCATAAGATAGAACGCGCATTCAAAAGTATCTTTTTTCCATTTCCCTTGTAAATGAAGGAATCAAATAGGAGAAAGGACAAATACACCTCGCAATGGATCTAAGACATCCTTTCAATTCCTTGTTGCAACATTTCTCCAAGCCTCCTATACAGAGCTTCTTGTTCATCGAATGATAGGTTCATCAATATCTGGTCCAAAATCATTTCAACCGATAAACTTTGTGGCACGACAAAGGACTGATGATATATATATGCAAACACGGCCATAACCATCTGGCAGTCCATTCTGTCGGTAATTCCACCGTGTCCTGGAATACTATCACCAAAATNCCYTTTTAATTTTAAAANCGTCCCCTTTTTAGAA
TARGET=650,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04649 Ps_contig_mira-and-tgicl-ass_franssen_34156
SEQUENCE=GTTGTGCAAAATCTAAATCTAAATTTTCTTCTAAAGAAAATGCTAAAGGCTAAAACATTTTCCTTTATCTTCAACCTGAAAGACATTTCTCACTAGATTTYCCTTGACAATTCTTGTGATAGTTGAGAAGAAGATTCACACTCTCTGGAGGCATTGAAGGATCTTTTGCAGTGTAAAATGGCTCCTTGAATCCCATCTTGTTGCTGTAACAGTGAATCATCACGGCGTTTAGACGACACTAACGCCGGCGGTGAAGCTGCCACTGCCGCTGACGCTGAACGGCTCTTTCCTAAATGTTTGTACACAACTCTAAGCCCTGCCGGAATATTCCCCTGTTTCAGGCTCTGATTCTGAGTACTCTTTGCACTGTTTTCTTCACTTTCAGGAACGGTTTCCCCTTCCTTTGCCACTTCACCTTCAACCTTGTTGTCAGTAGAAGCAGAACCAAACTTTCCTTCTTTTTCACCATGACTGTACCTACGAGAAACTCTAATGTATAGAGGCTTCACCATTTTTAAATACTTGTGCATAACTACTTCCTTGGAGAAACGTTTCTCTTCCAAAGGTGATAATGCTTGATGTTCTACTTTCTGGTTCGATTGGGTTTTGTTGTTGTTGACGTTTCCTTTGGAACTGTTGTCTCTAGTGAAAAGTGAAACAATAGGAACTTCTTCAATTTTGAATTTGATTCTATTTGGTTTTTGTTTCTTCTGTGTTATCACAGAATCGGTTTTTGGTTTTCTTAAGTCCAG
TARGET=607,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04650 Ps_contig_mira-and-tgicl-ass_franssen_34157
SEQUENCE=TAACAACTGGATGTGGACNNGCCAACGCCACTAGCAGTTCCTGTGGCAACAAAACTTGTTTTCATTAAATAAAACCAAACACACATAAAACTCGTGCGTCTTCTTAACACCTCACATTCCTTCATAAATTTAATAAGATAGTAAACCTTCCACCTCTCTCTCTTCACTCTCAGCTTTTCAAGTATGAATTTTCAACAACTTCCTCATCTCTTTCCGCAACAGATTCCTAATTTGAGAAATTCCAGTGTAATGAAGACTAATATTTCGAAGGAAGAGACTAAATGTGCACAGATGACAATCTTTTATGATGGGAAAGTAGTTGTATTTGATGATATTCCAGCTGAAAAAGCCAAGGACATTATGGTCTTTTCCGCCACCAATTATGTTCAAGAGCATCCTCAAGTAGCATCCTCACCTGTTATTTATGATTTACCAATGACTAGAAAAGCTTCACTTCATCGGTTCTTGGAGAAAAGAAAGGATAGAATTGGTGCAAGGGGACCATATCAAACAAGTAAACCAGCAGTGACTCTTGGTAAGCCAATTGATGAATATATGTCATGGCTTTCTTTGGCATCACAATCACCACAAGATCACAAATCTGAGTGTAGCTCTACCACTGTCTTGTTTTAACTTTTGTTGTTGGATTGGATAGATTCCAATATTGTCAGATGCTATGCTCACAAAAAATGTGTTGGCTCATGCTTATTAGTAACATTATTAAGAGTGTAGTTCTGAACTTCTTCTGATTGATATTAAAAAAAATGTACTTCTTGTTACTTACATTCAATTTGCAGAGTCGGTTAAATTTGGCACAGTTCAATTGGTTTTAGTAGCATATAT
TARGET=154,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2520
PRIMER_LEFT_PENALTY=0.095548
PRIMER_RIGHT_PENALTY=0.156479
PRIMER_LEFT_SEQUENCE=AAACACACATAAAACTCGTGCG
PRIMER_RIGHT_SEQUENCE=CCATAATGTCCTTGGCTTTTTC
PRIMER_LEFT=76,22
PRIMER_RIGHT=363,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.844
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.2594
PRIMER_LEFT_1_PENALTY=0.102945
PRIMER_RIGHT_1_PENALTY=0.156479
PRIMER_LEFT_1_SEQUENCE=ACACACATAAAACTCGTGCGTC
PRIMER_RIGHT_1_SEQUENCE=CCATAATGTCCTTGGCTTTTTC
PRIMER_LEFT_1=78,22
PRIMER_RIGHT_1=363,22
PRIMER_LEFT_1_TM=60.103
PRIMER_RIGHT_1_TM=59.844
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.3438
PRIMER_LEFT_2_PENALTY=0.187326
PRIMER_RIGHT_2_PENALTY=0.156479
PRIMER_LEFT_2_SEQUENCE=CACATAAAACTCGTGCGTCTTC
PRIMER_RIGHT_2_SEQUENCE=CCATAATGTCCTTGGCTTTTTC
PRIMER_LEFT_2=81,22
PRIMER_RIGHT_2=363,22
PRIMER_LEFT_2_TM=59.813
PRIMER_RIGHT_2_TM=59.844
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.3444
PRIMER_LEFT_3_PENALTY=0.095548
PRIMER_RIGHT_3_PENALTY=0.248820
PRIMER_LEFT_3_SEQUENCE=AAACACACATAAAACTCGTGCG
PRIMER_RIGHT_3_SEQUENCE=CGGAAAGAGATGAGGAAGTTGT
PRIMER_LEFT_3=76,22
PRIMER_RIGHT_3=215,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.751
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=140
PRIMER_PAIR_PENALTY_4=0.3518
PRIMER_LEFT_4_PENALTY=0.102945
PRIMER_RIGHT_4_PENALTY=0.248820
PRIMER_LEFT_4_SEQUENCE=ACACACATAAAACTCGTGCGTC
PRIMER_RIGHT_4_SEQUENCE=CGGAAAGAGATGAGGAAGTTGT
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=215,22
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_04651 Ps_contig_mira-and-tgicl-ass_franssen_34160
SEQUENCE=AGTTTAAAATANNTAAAAAAAATTAACTTATACATTTCCAATGCATTCACGGGTGAAGTTACAAACGCTGACATGCAAACACAGAGCATCACACGCAATCAAACAAATAGACAAAAAGAAACCAAACAGAAATTAATCAAATTTAAATGTTTTTGTCGTATTAATGCATGACGACGACAACTACTTCATGAAAGTGCAGAAAACTTTCTACTCTGCACCCAGCTACACAATACTTCTACTATGTCCTTGGCCCTTAATTTGTGGTACCCTACAACAACTTGATGAACGAGAAACAATGATCATTTTCTTCACTACATCTCTTTCAAACCTTGTGTACGTTAACCTTATTCCAAGCAACCTCTAAAATATGATCCATGCAATCAAAATATAAATGTACTAGCTCTATGAAACTATGATGATGGACACTTGAAACCTTCTGGTGGAGTTTTGCCACAGTCGATAAGAACCTGAAGAGCAAGGGGAATAACAAGGTTGATATTAAGAAGCTTGAGTCTAATAGTGGTGCATAGACACACAGCAGCATCCAAATCTACAAGTCCTTGAAGCAATGGACAACATGTTTGTTTAGCACTTCCACCGATTCCAATATGTATGAGACCACCGAGCACATCAACACATGCACCTAACTTCAAAGCATCAATGGAACATGTTGGTTGTGCTGGTGGTGGATAAGGTACAACTGTTGGTGGTGGTGGTGGACATGGTGTTTCACTAGGTGGACTAGGAACTGGTACGGTCGGTGTCGGTGGTGTAATAATAGGCGGTGTTGGTACAAAAGGTGGTGTTACTGGAACGACAGGAGGAAGAGGAACATAAGGTGGTGTTACTGGAACAACTGGTGGAAGAGGAACATGTGGTGGAAAAACAATTGGTGGCTTAGGAACATAAGGCGGTGTTACCGGAACAACTGGTGGAAGAGGAACATGTGGTGGAAAGACAATTGGTGGCTTCGGAACATAAGGCGGTGTTACTGGAACAACCGGAGGAAGAGGAACATGTGGTGGAAAAACAATTGGTGGTTTAGGAATATAAGGTGGTGTTACTGGAACAACAGGCGGTTTAGGGACATGAGGTGGTTTCACAACTGGTGGTTTAACAACTGGTGGTTTTGGTACATAAGGTGGATGCACTATAGGTGGATGCACTACTGGTGGTTTTGGAACATGGGGTGGATGCACTGCTGGTGGTTTTGGAACATGAGGTGGATGCACTGCTGGTGGTTTGGGATAATGAGGAGGTTTTGGAACATGTGGTGGTTTCGGTGATGAAGAAGGAGGTGGACAAGGTTGTGGCTTTGGTGGTTTATGAACTGGTGGTTTAACAATAGGAGGTTTATGTGTTGGTGGCTTAGGAGAAGGGTAGGGACAATAAGGACAAGCAAGAACATTGAGTAAGGTACTCAAGTTCAAGAGAAGTATCAAAACATTGGCTATAGCAAAGTTAGCCATGTTTCGAAGATTTTAGTGTAATGTGTGTGCTCACTACTATGAAGCTTTGCACTTCCCCC
TARGET=705,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0386
PRIMER_LEFT_PENALTY=0.022302
PRIMER_RIGHT_PENALTY=0.016312
PRIMER_LEFT_SEQUENCE=TATGTATGAGACCACCGAGCAC
PRIMER_RIGHT_SEQUENCE=GTTCCTCTTCCACCAGTTGTTC
PRIMER_LEFT=607,22
PRIMER_RIGHT=871,22
PRIMER_LEFT_TM=60.022
PRIMER_RIGHT_TM=60.016
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.1217
PRIMER_LEFT_1_PENALTY=0.022302
PRIMER_RIGHT_1_PENALTY=0.099438
PRIMER_LEFT_1_SEQUENCE=TATGTATGAGACCACCGAGCAC
PRIMER_RIGHT_1_SEQUENCE=ACATGTTCCTCTTCCACCAGTT
PRIMER_LEFT_1=607,22
PRIMER_RIGHT_1=875,22
PRIMER_LEFT_1_TM=60.022
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=0.1217
PRIMER_LEFT_2_PENALTY=0.022302
PRIMER_RIGHT_2_PENALTY=0.099438
PRIMER_LEFT_2_SEQUENCE=TATGTATGAGACCACCGAGCAC
PRIMER_RIGHT_2_SEQUENCE=ATGTTCCTCTTCCACCAGTTGT
PRIMER_LEFT_2=607,22
PRIMER_RIGHT_2=873,22
PRIMER_LEFT_2_TM=60.022
PRIMER_RIGHT_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=0.1439
PRIMER_LEFT_3_PENALTY=0.022302
PRIMER_RIGHT_3_PENALTY=0.121635
PRIMER_LEFT_3_SEQUENCE=TATGTATGAGACCACCGAGCAC
PRIMER_RIGHT_3_SEQUENCE=CTAAGCCACCAATTGTTTTTCC
PRIMER_LEFT_3=607,22
PRIMER_RIGHT_3=900,22
PRIMER_LEFT_3_TM=60.022
PRIMER_RIGHT_3_TM=59.878
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=0.1439
PRIMER_LEFT_4_PENALTY=0.022302
PRIMER_RIGHT_4_PENALTY=0.121635
PRIMER_LEFT_4_SEQUENCE=TATGTATGAGACCACCGAGCAC
PRIMER_RIGHT_4_SEQUENCE=CCTAAGCCACCAATTGTTTTTC
PRIMER_LEFT_4=607,22
PRIMER_RIGHT_4=901,22
PRIMER_LEFT_4_TM=60.022
PRIMER_RIGHT_4_TM=59.878
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_04652 Ps_contig_mira-and-tgicl-ass_franssen_34163
SEQUENCE=ACGTTTTAAAAACTAATACTAACTTCTGCYTATTTTGTTGTTTTCTGCTATTACATGTTTTGTTTGGAGTTTCCACTATATTATGTTGTCTGTTTAATGACCTAACACTGCAGTGTTCATTATATACACACACACGAAGCATGATCTACTTCCACTACTAAAACTTTCTACAATTTCTTTATTTATCTTACAAAAATGTACTATTCAGTAGCTACAAACTTTGGGTGTTGCTTGTTTCTTGATGAAGATGAGGAAAGAACGAATTCACCATTGGACAATCGTAATTCAGTTCAGCAGGCCTTGCAATTTTATGCCATTCTTGATCAATCAACATTTCATCCGTCAATCCAAATTCTCTTAACTCTTGTTCATCGTGATGCATCAGTAAACTATACCTTCCACCATAACCCTTTTCCATGACAAGCTCACCTCCATTTCCAAAGTTATTGAGACCTTCAAACTGTTCAACTGTCCACTTATACCACCTCATGAGAAAAACTCTAAGTGTTAGACCGTGCGACACGATCACCAAGTTCACATCAAAATTCTTCTCCCCCGGAGGCTGATATCGTCCGATATTAATATCGGCTCGTAGTGTTTCTCTAAACCCTGTGATTCTATCATATACATCAGCCGCGGATTCTCCATTTGGAAATCGATAAAAGAATCGACCATAAAGATGTCGTTGCGCCTTTTCAACTTTCATCAACTCTCTATTTTGAAAATTCCCGAAATCTTGTTCTCGAATGCGAGGCTCTTCTCTAAAACCAGCAATTCTGGAGCGTTCAAAAGGACGAGCCAAGCTTTGCAAAGTTTCAAGAGTTCTTCTATATGGAGACACATAAAAGTAGAGTTGCCAATTTTCATCACCATCTTTCTGAATCATGTTCTTGATTCTTTGACCACATTCCTCTGCTTGAACTTTTCCTTTGTTGGTTAGGCCTATTTTAGGATCAGGTACCCTTGTGTAAACACTCTCATCCACGTTTCCTTCACTCTCTCCATGTCTAACAAGGATTATTCTTCTAGGCCTTGGAGGTACTGCCCCAAAAGTGGAGGCTACTAAAGGGTTTATGAGAGGATTTTTCTCTGGAAACCTGGCATGTCCATTGTTGTTGTTAAGTGAATCTATGGATTCTGTTTGGTTGTGACAACATTGGATTAGTACTAAGTATGTTTTGTTTGTCTTAGAATACTTAGAAATAGGATTGGATTTGATTTTAAGGTGTTTTGGTTGAGATGCTGAGAGAGGTAGTGTTGCTATGGCAATGCTCATGATATACTTCCCCGCG
TARGET=126,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04653 Ps_contig_mira-and-tgicl-ass_franssen_34165
SEQUENCE=TTTGGTTTTAAGGTTTAGGCTTTTGTCGGAAGTCCAAAAGGACGAAAGAACRRNNYGGTTTGGTTGCATACAACATGTGGAACGCCGGCTTATGTAGCTCCTGAAGTCATCAAGAGGAAGGGTTATGACGGTGCCAAAGCCGATATTTGGTCTTGTGGAGTTGTTCTCTTTGTGTTACTTGCTGGTTATGTGCCTTTCAATGACTCGAATTTGATGGAGATGTATAGGAAGATAAGTAACGCGGAGTTTAAGTTTCCTAATTGGTTTGCGAAAAACGTTAGGAAGCTGTTGTGTAAGATGTTGGATCCGGATCCTAGCACTAGGATTTCTATTGAGAAGATCAAAAAATGTTCTTGGTTTAAGTATGGACCGAATGGTAGACCGAAACAACATGAAGAGGAAAACAAGAGTATCTCTTCTTTGGATACAAATTCTGTAGGATCGACAGTTCTGATTGAAGGTGTGTCACGTGACACGACACAGACACATGTGACTGATGAAATTGAAAAGCAAGAGTCAATTGTACCGATGAGTATAAATGCGTTTGATATCATATCTCTTTCTAATGGTTTTAATCTAGGTGGATTCTTTGAAGACAGTTTTAAGAAAAGGGAAGCGAGGTTTACTTCAAGACAACCCGCGTCGGTTATCATTTCAAGGCTGGAAGAGATTGCTAAGAGACTGAAGATGAAAATAAAGAAAAGGGCTGCTGGTTTGTTGAAACTAGAGGGAGTTGATGAAGGTAGGAAAGGGATTTTGTCTATTGATGCTGAGATATTTGAGGTTACTCCAGTTTTCCATTTGATTGAAGTGAAGAAATCAAATGGAGATACATTGGAATATCAGAAAATATTGAATGAGAAAATTAGGCCTTCTCTTCAAGATATTGTTTGGGTTTGGCAAGGTGAAATACAAGAACAATCACAAGAGTCGGAGCAGGAGCAGTAGCAGCAGCAGCAACAATCGTGCTATCTTCATTTTGTACATTTCAGGATATACTTTAGTTTTTTTTCCCGAAATTTATGCTTGTTTCTGCATAGTCTTTTTATGTGTTTAACTATGACATTAACCTTAATGTATT
TARGET=947,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04654 Ps_contig_mira-and-tgicl-ass_franssen_34175
SEQUENCE=GATGAAGCGCGATTGCGAAAAGCGCTTTTCAACGCGTCTTTAACCGTTCGCGGCGGTGATGAACGGTGACAACGGAGGATATCAACAAGCGCATAAACGATCTCGCGTTTGGATCCAACGACAGTACGGTAATCATCGACAGACGAAAGAAGGCTGTGAATTGTAGCGGCGGCGGATTGAACAGCGGCGGCGGCGGAGGTGGAAGAATGATGAGATATAACGTGTGCAAGCGCGTCGAGAACACCGCGTGTGGACATGATAGGTTCTTTTTCTGTTATGGAAAGGTTAAGAAGCGTTGCGGTTGCGTTTTCTTGCGACGGATGAGAAGAAGAATAGAGAGTTTCTGCTAGGTACGGAATCGCGCCTGATTCTGTAATTACGGGTCGGGTTTCCGGTCTTGTTTTGACATTTGCCGAAGCTCGCAGAGAGCTTCGATTCGGGTTCGTTGGGAAACGGAGCTTAGCTTTGAGACTAGGTTCCGCACGGCGCGGTGTTTTGTGTCCATCTTGGTATAGAGGGAGGTTCGGTGGGTGGTGTTGGTTTGGGAATTGGTGCGGTGGTTGGTTTGGAGGGAGATGGAGAAAAAACGCGTAGTGGTTTTTGGGGAAAGATTATGAGAATGGGTTTATGAAGATTCGAAAGGCAAACACGTCTTAACAACTTTTTATGTGACCAAAAGT
TARGET=184,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4338
PRIMER_LEFT_PENALTY=0.171757
PRIMER_RIGHT_PENALTY=0.261998
PRIMER_LEFT_SEQUENCE=ACGAAAGAAGGCTGTGAATTGT
PRIMER_RIGHT_SEQUENCE=ATGTCAAAACAAGACCGGAAAC
PRIMER_LEFT=142,22
PRIMER_RIGHT=408,22
PRIMER_LEFT_TM=60.172
PRIMER_RIGHT_TM=60.262
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.4741
PRIMER_LEFT_1_PENALTY=0.140832
PRIMER_RIGHT_1_PENALTY=0.333229
PRIMER_LEFT_1_SEQUENCE=GTGACAACGGAGGATATCAACA
PRIMER_RIGHT_1_SEQUENCE=CAACGCTTCTTAACCTTTCCAT
PRIMER_LEFT_1=65,22
PRIMER_RIGHT_1=297,22
PRIMER_LEFT_1_TM=59.859
PRIMER_RIGHT_1_TM=59.667
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.5050
PRIMER_LEFT_2_PENALTY=0.171757
PRIMER_RIGHT_2_PENALTY=0.333229
PRIMER_LEFT_2_SEQUENCE=ACGAAAGAAGGCTGTGAATTGT
PRIMER_RIGHT_2_SEQUENCE=CAACGCTTCTTAACCTTTCCAT
PRIMER_LEFT_2=142,22
PRIMER_RIGHT_2=297,22
PRIMER_LEFT_2_TM=60.172
PRIMER_RIGHT_2_TM=59.667
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.6817
PRIMER_LEFT_3_PENALTY=0.419726
PRIMER_RIGHT_3_PENALTY=0.261998
PRIMER_LEFT_3_SEQUENCE=TCCAACGACAGTACGGTAATCA
PRIMER_RIGHT_3_SEQUENCE=ATGTCAAAACAAGACCGGAAAC
PRIMER_LEFT_3=113,22
PRIMER_RIGHT_3=408,22
PRIMER_LEFT_3_TM=60.420
PRIMER_RIGHT_3_TM=60.262
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.6827
PRIMER_LEFT_4_PENALTY=0.349469
PRIMER_RIGHT_4_PENALTY=0.333229
PRIMER_LEFT_4_SEQUENCE=GATATCAACAAGCGCATAAACG
PRIMER_RIGHT_4_SEQUENCE=CAACGCTTCTTAACCTTTCCAT
PRIMER_LEFT_4=77,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=59.651
PRIMER_RIGHT_4_TM=59.667
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_04655 Ps_contig_mira-and-tgicl-ass_franssen_34178
SEQUENCE=TCTTTTGATCAGCCAGAGTTCTTTAGAGTTCCTCTAATATATCCCCTGTATCAGGAAGACTTAATCGCTGATCATTAGCTGGTTGATCGATGAGTTCTTGTAACTTCTGCTTTATGTCAAGAACTTTATCAGATGGCTTGCACCGGATGAAATACGTTGTCTTACTACGCTTAACACGAATGTACATATCCATTTGCTATGGTTCAGGCGTTTCTACTGAGAAACGAAATTGAAAGCTCCTGCAATCCACAATTGATGAATTGATTTAAGGAATTTGAGAGGGAGAGAAATAGAAATCGGTGAAAATGAAATTTGAATTCGAAAGTAAGATTGAGAGAGAGAGAGAATTGTTGTTCTTACCCGGGAAGGGGTGAAAATTTTCGTGTCTCCCCGC
TARGET=333,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1922
PRIMER_LEFT_PENALTY=0.156135
PRIMER_RIGHT_PENALTY=2.036112
PRIMER_LEFT_SEQUENCE=GCTATGGTTCAGGCGTTTCTAC
PRIMER_RIGHT_SEQUENCE=CTTCCCGGGTAAGAACAACA
PRIMER_LEFT=195,22
PRIMER_RIGHT=367,20
PRIMER_LEFT_TM=60.156
PRIMER_RIGHT_TM=59.964
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=2.2728
PRIMER_LEFT_1_PENALTY=0.236697
PRIMER_RIGHT_1_PENALTY=2.036112
PRIMER_LEFT_1_SEQUENCE=GAAACGAAATTGAAAGCTCCTG
PRIMER_RIGHT_1_SEQUENCE=CTTCCCGGGTAAGAACAACA
PRIMER_LEFT_1=220,22
PRIMER_RIGHT_1=367,20
PRIMER_LEFT_1_TM=60.237
PRIMER_RIGHT_1_TM=59.964
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=148
PRIMER_PAIR_PENALTY_2=2.3840
PRIMER_LEFT_2_PENALTY=0.156135
PRIMER_RIGHT_2_PENALTY=2.227860
PRIMER_LEFT_2_SEQUENCE=GCTATGGTTCAGGCGTTTCTAC
PRIMER_RIGHT_2_SEQUENCE=CTTCCCGGGTAAGAACAACAA
PRIMER_LEFT_2=195,22
PRIMER_RIGHT_2=367,21
PRIMER_LEFT_2_TM=60.156
PRIMER_RIGHT_2_TM=61.228
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=2.4646
PRIMER_LEFT_3_PENALTY=0.236697
PRIMER_RIGHT_3_PENALTY=2.227860
PRIMER_LEFT_3_SEQUENCE=GAAACGAAATTGAAAGCTCCTG
PRIMER_RIGHT_3_SEQUENCE=CTTCCCGGGTAAGAACAACAA
PRIMER_LEFT_3=220,22
PRIMER_RIGHT_3=367,21
PRIMER_LEFT_3_TM=60.237
PRIMER_RIGHT_3_TM=61.228
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=2.4918
PRIMER_LEFT_4_PENALTY=0.156135
PRIMER_RIGHT_4_PENALTY=2.335710
PRIMER_LEFT_4_SEQUENCE=GCTATGGTTCAGGCGTTTCTAC
PRIMER_RIGHT_4_SEQUENCE=TTCCCGGGTAAGAACAACAA
PRIMER_LEFT_4=195,22
PRIMER_RIGHT_4=366,20
PRIMER_LEFT_4_TM=60.156
PRIMER_RIGHT_4_TM=60.336
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_04656 Ps_contig_mira-and-tgicl-ass_franssen_34186
SEQUENCE=CGAAGGTCCAGGAAGCACCGAACCAGTCAACGACAGAATATCAAATCTCCCCGGAAGCGCCACCACAGCTCCAGGACCTGTAGGCTGCCGGAGAGAAACATTAACAACGGTTCCACTTGCGCTCAGAATGCAAATACCTCTCTGTCGTTTCCTAGCGAACTGAACAACACTATCAGCTATATCAGTCCCTGACGCTACTTCCATCACATGGCTTCTAAGCGCGTTAGGGCTGTCTCTTGTTATAAATATCGGTGGCTTCGGTTTGTTTTTCGACCCTGACGGACGACCTCGTGGACGGCGCGTGGAGTTGTGTGTTTCTATAGCTCCTCCTTTTTGTTCATCGCTGTTCTCTCTCTCATCCTCTTCATTGTTATTGTTATTGTTATTCGCTCCTGTCACCGCACTTTCGTTCATGGAAAAACCCAGATCCGGTTTTGGTACTAGTGGTGATGAGTTGACCGAGTTATCCATCCCGCCTAAACCCACCGGTCCAGTCCACCACCGGTTCGCCATCACACTTCACCTAGAAACTAGAAACCAGTCACAAGAAATCTCAAACCTAATTCACTCCTAATAAGTCAAA
TARGET=348,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3454
PRIMER_LEFT_PENALTY=0.178178
PRIMER_RIGHT_PENALTY=0.167242
PRIMER_LEFT_SEQUENCE=TTATAAATATCGGTGGCTTCGG
PRIMER_RIGHT_SEQUENCE=GGATGGATAACTCGGTCAACTC
PRIMER_LEFT=239,22
PRIMER_RIGHT=472,22
PRIMER_LEFT_TM=60.178
PRIMER_RIGHT_TM=59.833
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.3490
PRIMER_LEFT_1_PENALTY=0.178178
PRIMER_RIGHT_1_PENALTY=0.170871
PRIMER_LEFT_1_SEQUENCE=TTATAAATATCGGTGGCTTCGG
PRIMER_RIGHT_1_SEQUENCE=CTCGGTCAACTCATCACCACTA
PRIMER_LEFT_1=239,22
PRIMER_RIGHT_1=462,22
PRIMER_LEFT_1_TM=60.178
PRIMER_RIGHT_1_TM=60.171
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.4342
PRIMER_LEFT_2_PENALTY=0.266928
PRIMER_RIGHT_2_PENALTY=0.167242
PRIMER_LEFT_2_SEQUENCE=TATAAATATCGGTGGCTTCGGT
PRIMER_RIGHT_2_SEQUENCE=GGATGGATAACTCGGTCAACTC
PRIMER_LEFT_2=240,22
PRIMER_RIGHT_2=472,22
PRIMER_LEFT_2_TM=59.733
PRIMER_RIGHT_2_TM=59.833
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.4378
PRIMER_LEFT_3_PENALTY=0.266928
PRIMER_RIGHT_3_PENALTY=0.170871
PRIMER_LEFT_3_SEQUENCE=TATAAATATCGGTGGCTTCGGT
PRIMER_RIGHT_3_SEQUENCE=CTCGGTCAACTCATCACCACTA
PRIMER_LEFT_3=240,22
PRIMER_RIGHT_3=462,22
PRIMER_LEFT_3_TM=59.733
PRIMER_RIGHT_3_TM=60.171
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.4609
PRIMER_LEFT_4_PENALTY=0.080323
PRIMER_RIGHT_4_PENALTY=0.380537
PRIMER_LEFT_4_SEQUENCE=TTTCCTAGCGAACTGAACAACA
PRIMER_RIGHT_4_SEQUENCE=GGTGACAGGAGCGAATAACAAT
PRIMER_LEFT_4=147,22
PRIMER_RIGHT_4=399,22
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=60.381
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_04657 Ps_contig_mira-and-tgicl-ass_franssen_34192
SEQUENCE=CCGTAATTATTCGCCGNNCCACTCCCACCCGCCCTAACTTGGGGTTGGCCTCCCACCGGCCAGCTCCGCCTCCTAATACTCCGTACTGGCACCAACGAGCTCAGAATACACCAACCGCAGGAACACCGTGCTTTCCACAGCCTCTGACAACAACGAGATTTGCCTCTCCAACTGTTCCGGGCATTCCTCCACCACACCCCATGTACCAAGTAGATCCCGGCATGGCCGTCACCGCCGCTCAACCATCTCCTCCGCCACACTTAGACTTCCATCCGTCAAAGGAGAGCATAGACGCAGCTATTAGTGATGTATTATCAAAACCATACTTACCATTGCCACTTGGTCTTAAAGCTCCTGCACTTGAAGGTGTGATGGGTGAGTTACAGAGACAAGGAATTCCGAGAATTCCACCCTCTTGTGCTTGAACGAGAGAGAATGGTTTTGCTTCTTATGCATGACAAGGCCATGAAACCCTAATTCGACGAATTTTCATATTCTCACTTCTTTTTTTTGTTTGTTTGAACAAAAAGAGTGAGGGACAATAATATTAAGAACTTTCTTTGTTCATATAATATATCTAAGCAGCGCAAAAAGTTTAACTCGAGCTTTTGGTATATAGTATTTAACCTAATGCCAAATGTGTGTGGGATTATAAGGCTGTGATTTTTGTATTGTTCGAGGAAGATGATATTATCTACGAGACTAAATATGTGAGGAAATATCAATAAAGGGCTACTTTTTACTARTAAA
TARGET=510,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04658 Ps_contig_mira-and-tgicl-ass_franssen_34193
SEQUENCE=GTGAGCTAAATACAAATTCAGTAGCACACGTAAAATAAACACTTCAATTTAGCCGTTTCAAAAGCAATGGCTTTACATGATTTCTCAAATGATATCATCATTCATCTAAAACTGCTACACAATCATTCAACAAACAAACAAAAGTTGGATCATTACAAGAAATCAGAAGATCGCCACTTTAATTAATATCCACTATACCAAAAGAGAAAAAAAAACTGAAATGGCAAAAGTGTGAAACATGAAACATTAACCTACATCACATGTGTAAAGTGCTGAGATTAAGACTCTCGCGGAGAAAATGAAACTAATATCTAGAACTCTTCATTCCATCTCCAAAACTCTCCGGGGTTACAAGCTACAGAACAGGGTGGCTTGAACACCAAACTTAAGATACCAAAGGCATTTTTGCATAGGCATTCAAATCGATGTACTCTGCTAGAAGGAAATCGTGCTTTGCCTGAAATTTGTTTAGTGGTACTGCCAAAAAAGGACTTGAGAATTGATCTCAGACTCCTTTCCAGAAAACAAACTAAAATTCATCTTTGGGGTACTTGTCACCTTTCCCTAGTTGAAAAAACCACTGAAGTATTTGGAATATCTTGCTGCCCGAGGGCTTCTGCTCTAGCTTTGCATTACAGACTGTATCGTAGTCATGCTTCCGATGAGGAGCAACTATTTTTTCCTTGAATACTTCAAATGGACAGAAATCAGAACCATTACAACCCGGCATTGGAATGGGGTGTTCATTGTGCAGCACTTGCACAAAGTGCTTGCTTCTAGCTTTGTCTGGCGCAGGACATCTATACAGGACCAGCATGTTGTTACCAGCAAAAGGAGCCACAATGCTACCCCTCCATTTTCTTTTCTGTGGAGGCTTTAGAGGAAATGCCAAAGGTATCTCCTTCTGGATTGTGTCAAACTCAGACCCTTCAAGAAATAAACCAAGCAGACATGAGAATGGGACTACAGTTTCAGCATGTGCAAACCGAAGTCTTGCTTTTTCAAAGCTGCCAGGCACATGTTTTTCTTCTTTAGCCATGATAGCTTGCTCCATGGATTGAACAACATCTTCAAGTAATGGCATCCCCATTCTATAATTTATTGATTTACCATAACCCTTCAGAATGTACATTTCTAAGTCATCTGTCCACTCCAGCAATGTAATCTCAGATGGGCTGAAAAGACTACAAGCTTGATCAGTTATATCCAACAAGGATGCTTCCTCTTTACATAAGAACCAAAGAGATGCTGTAACCTGCCTTGTAAAATTCAGTCCATAACGCCCAACTAATGCAGATGTAATCTCATCCAAGGTAGGTTCCTTAAGTTTATCAACTGCGGGCTCCTGCCTTTTCCTGAAATCCTTGTAGTTTTGACAACATTCATGAAATCTCAGCATGGTGTCACTAGCACGGTTTTCACTTATAACAGAAAGGCTCTGTGCTCCCCAGGTCCAAGAGTTCCATTGCCACTGAAAATACCCATTCCAAATGCGACAGCACTAGCTGATGCTCGGGGAATCTGAGTTGCCTTTATAGGATATATGTCTGGATGGTAATC
TARGET=129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.0559
PRIMER_LEFT_PENALTY=3.042469
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=TAGCCGTTTCAAAAGCAATG
PRIMER_RIGHT_SEQUENCE=TTTCTCCGCGAGAGTCTTAATC
PRIMER_LEFT=49,20
PRIMER_RIGHT=297,22
PRIMER_LEFT_TM=58.958
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=3.1023
PRIMER_LEFT_1_PENALTY=3.042469
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=TAGCCGTTTCAAAAGCAATG
PRIMER_RIGHT_1_SEQUENCE=GGAGAGTTTTGGAGATGGAATG
PRIMER_LEFT_1=49,20
PRIMER_RIGHT_1=343,22
PRIMER_LEFT_1_TM=58.958
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=3.3055
PRIMER_LEFT_2_PENALTY=3.292112
PRIMER_RIGHT_2_PENALTY=0.013401
PRIMER_LEFT_2_SEQUENCE=TAGCCGTTTCAAAAGCAATGG
PRIMER_RIGHT_2_SEQUENCE=TTTCTCCGCGAGAGTCTTAATC
PRIMER_LEFT_2=49,21
PRIMER_RIGHT_2=297,22
PRIMER_LEFT_2_TM=62.292
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=3.3116
PRIMER_LEFT_3_PENALTY=3.042469
PRIMER_RIGHT_3_PENALTY=0.269153
PRIMER_LEFT_3_SEQUENCE=TAGCCGTTTCAAAAGCAATG
PRIMER_RIGHT_3_SEQUENCE=AAAGTGGCGATCTTCTGATTTC
PRIMER_LEFT_3=49,20
PRIMER_RIGHT_3=179,22
PRIMER_LEFT_3_TM=58.958
PRIMER_RIGHT_3_TM=59.731
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=3.3520
PRIMER_LEFT_4_PENALTY=3.292112
PRIMER_RIGHT_4_PENALTY=0.059875
PRIMER_LEFT_4_SEQUENCE=TAGCCGTTTCAAAAGCAATGG
PRIMER_RIGHT_4_SEQUENCE=GGAGAGTTTTGGAGATGGAATG
PRIMER_LEFT_4=49,21
PRIMER_RIGHT_4=343,22
PRIMER_LEFT_4_TM=62.292
PRIMER_RIGHT_4_TM=59.940
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_04659 Ps_contig_mira-and-tgicl-ass_franssen_34194
SEQUENCE=CTTTTTCTTAAACCTTTCAACAAATCCCCAAACCTAAAATTTCAATCCAACAAACGCTTTCAAGTTGTTTCTTTCTCCGGAAACCCGGATCCGAATAGCGGATTTTTGGTTTCCGGCGAGAATTATAATGTCTGCAGCACTAGAGATGTTCGGTCTTATGCGGGTCGTAGCAAGAAGACGGGTGGCGCATCGTCTTCCGGTGGTAGACTTGAAGGCAATGCGGATTTTCGGAGACGATTGAGGCGGAGTGCTAGGGCGAAAAGTAAGAAGCTTGCTGAGTCTTTGTTCTATCGGTTGAAAAACCCTAAGGGGCGTGGGAATTATCCGGATAATTTCTCTGAGGATTTGCTTCAACAAATTGGGCTTGGATATGATCGACTGGTTCGGTTCATGGATAAGGATGATCCGAATTTACGCCACCCGTATGATTGGTATAAATATGGTGAATATGGGCCGTATTCTTGGCGTGGGATTGTTGTTGGTGAGCCAGTTAGTGGCGGGATTACGGATGAGTGTGTGACGATAATTAGTGAAGTGAAGGATCATGAAGAGTGGGAGGAGATTGAGAAATATGATATGGCTGCTGATTTTGGGGAAAAGGTGAAGCAGTTGGATCAAACGAAATTAAGGTATTTTTGGGTTTTCGTTAGGCATCCGAAGTGGAGAGTTTCGGAACTACCTTGGCAGCAATGGACATTAGTTAGTGAGGTAGTGCTTGAAGCTGGTAAGCAGAGGTTAGATAAGTGGACTTTGATGGGTAGACTCGGGAACAAAGCGAGGTCGTTAGTAGGACAATGTGCAGCGTGGATGAGACCTGATATTGTGTATGTTAAGAGGCCGGTGTTTCAGTGTAGGTTTGAGCCGCAGGATAACTTTTTTAATGCGATAATTCCGTTTCTTGATCCTAGTACCGAACAGGATTACCTTTGCGAGCTGCAAAATGATGATGGCAGTGTTGAGACGTGTACTTATTATGGTGGATTGTGTAAAATTGTGAAGGTGAATCAAAAGGCTTTTGTTGACGATGTGGTGAATGCGTATCAGAAATTGAGTGACGAGAAAAAGTCGAAATGTTTGGAGTTTCTTTTGGGCCATCATCCGGTGGAAATTTTGCATCCGTATACTAAAGAGTGGAAGGCTAAGTTGGAAGAGATGGAGCTTGGTTGTGATGCTCCGGATGAAGAAGATGATGATGTTGTTGGTGATAATGATACTGAGATTCTTGATTGGATTGAGGATGAAGGTAGCGACGGAGAAGCTGAATTCGATGGTGATGATGATGATGATGAGAATGATGAAGAAGAGGAAGAGGAAGAGGAGGAGGAGATTGATGATTTTGATGTTAACGAAGACCAAAATCTAGTTACGGACATGGAAGAAAGCGAAGATGGTAAATTTCACGCGATGGACGAAGACGAGAAGGACTGGAAGGAGTTTCGAAAATCAACAAATAGTGCCGAGGCAATGGAAAACATGGCAAGAAAGAGTGTCGAGCTCTCTACAAAGTTATACAAGAAGCAGATGATGACGGCGGAGAAGGCAGAGAAAGAAACAATGCACGATTCAGTAGATGGAGAGGAAACCGCCTTGAGAGGAAAGCGAGCAAAGGTCGGTCCAGAAGAGTGGAAGTATGTTGGGCTCGGTCCGTGGAGAAAAAAGATCAAGAAGAGCAAACTTCCTCCGGACCAATTTCTGCGAGCAGCTATTCGGCCTTTCACTTACAGAAATCTTGTTAAAGAGATTGTTTTAACTAGGCATGCAATTTTGGATGGCGATATTGGTAAGAAAGAGTAAGAATATAGTAATGTTGAGAAAGTTAGTAGAATGAATCATTGTCCTTGCTTTTGTGTAAGGATTTTTTGTTATGCTTACCAAGTTTCTGAAAAAGATTATGACAACATACAACATCGATTTATGTATACANGTTCTTCTCTGTGTTTTCAAANGATTTTGATGTTAG
TARGET=1272,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0698
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.039531
PRIMER_LEFT_SEQUENCE=TGAGTGACGAGAAAAAGTCGAA
PRIMER_RIGHT_SEQUENCE=ATCAATCTCCTCCTCCTCTTCC
PRIMER_LEFT=1048,22
PRIMER_RIGHT=1331,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.1786
PRIMER_LEFT_1_PENALTY=0.139083
PRIMER_RIGHT_1_PENALTY=0.039531
PRIMER_LEFT_1_SEQUENCE=AGAGATGGAGCTTGGTTGTGAT
PRIMER_RIGHT_1_SEQUENCE=ATCAATCTCCTCCTCCTCTTCC
PRIMER_LEFT_1=1148,22
PRIMER_RIGHT_1=1331,22
PRIMER_LEFT_1_TM=60.139
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.1822
PRIMER_LEFT_2_PENALTY=0.139083
PRIMER_RIGHT_2_PENALTY=0.043085
PRIMER_LEFT_2_SEQUENCE=AGAGATGGAGCTTGGTTGTGAT
PRIMER_RIGHT_2_SEQUENCE=TCCTCCTCTTCCTCTTCCTCTT
PRIMER_LEFT_2=1148,22
PRIMER_RIGHT_2=1321,22
PRIMER_LEFT_2_TM=60.139
PRIMER_RIGHT_2_TM=59.957
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=0.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.1822
PRIMER_LEFT_3_PENALTY=0.139083
PRIMER_RIGHT_3_PENALTY=0.043085
PRIMER_LEFT_3_SEQUENCE=AGAGATGGAGCTTGGTTGTGAT
PRIMER_RIGHT_3_SEQUENCE=CCTCCTCTTCCTCTTCCTCTTC
PRIMER_LEFT_3=1148,22
PRIMER_RIGHT_3=1320,22
PRIMER_LEFT_3_TM=60.139
PRIMER_RIGHT_3_TM=59.957
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=0.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=173
PRIMER_PAIR_PENALTY_4=0.2345
PRIMER_LEFT_4_PENALTY=0.139083
PRIMER_RIGHT_4_PENALTY=0.095445
PRIMER_LEFT_4_SEQUENCE=AGAGATGGAGCTTGGTTGTGAT
PRIMER_RIGHT_4_SEQUENCE=TACCATCTTCGCTTTCTTCCAT
PRIMER_LEFT_4=1148,22
PRIMER_RIGHT_4=1392,22
PRIMER_LEFT_4_TM=60.139
PRIMER_RIGHT_4_TM=60.095
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_04660 Ps_contig_mira-and-tgicl-ass_franssen_34194
SEQUENCE=CTTTTTCTTAAACCTTTCAACAAATCCCCAAACCTAAAATTTCAATCCAACAAACGCTTTCAAGTTGTTTCTTTCTCCGGAAACCCGGATCCGAATAGCGGATTTTTGGTTTCCGGCGAGAATTATAATGTCTGCAGCACTAGAGATGTTCGGTCTTATGCGGGTCGTAGCAAGAAGACGGGTGGCGCATCGTCTTCCGGTGGTAGACTTGAAGGCAATGCGGATTTTCGGAGACGATTGAGGCGGAGTGCTAGGGCGAAAAGTAAGAAGCTTGCTGAGTCTTTGTTCTATCGGTTGAAAAACCCTAAGGGGCGTGGGAATTATCCGGATAATTTCTCTGAGGATTTGCTTCAACAAATTGGGCTTGGATATGATCGACTGGTTCGGTTCATGGATAAGGATGATCCGAATTTACGCCACCCGTATGATTGGTATAAATATGGTGAATATGGGCCGTATTCTTGGCGTGGGATTGTTGTTGGTGAGCCAGTTAGTGGCGGGATTACGGATGAGTGTGTGACGATAATTAGTGAAGTGAAGGATCATGAAGAGTGGGAGGAGATTGAGAAATATGATATGGCTGCTGATTTTGGGGAAAAGGTGAAGCAGTTGGATCAAACGAAATTAAGGTATTTTTGGGTTTTCGTTAGGCATCCGAAGTGGAGAGTTTCGGAACTACCTTGGCAGCAATGGACATTAGTTAGTGAGGTAGTGCTTGAAGCTGGTAAGCAGAGGTTAGATAAGTGGACTTTGATGGGTAGACTCGGGAACAAAGCGAGGTCGTTAGTAGGACAATGTGCAGCGTGGATGAGACCTGATATTGTGTATGTTAAGAGGCCGGTGTTTCAGTGTAGGTTTGAGCCGCAGGATAACTTTTTTAATGCGATAATTCCGTTTCTTGATCCTAGTACCGAACAGGATTACCTTTGCGAGCTGCAAAATGATGATGGCAGTGTTGAGACGTGTACTTATTATGGTGGATTGTGTAAAATTGTGAAGGTGAATCAAAAGGCTTTTGTTGACGATGTGGTGAATGCGTATCAGAAATTGAGTGACGAGAAAAAGTCGAAATGTTTGGAGTTTCTTTTGGGCCATCATCCGGTGGAAATTTTGCATCCGTATACTAAAGAGTGGAAGGCTAAGTTGGAAGAGATGGAGCTTGGTTGTGATGCTCCGGATGAAGAAGATGATGATGTTGTTGGTGATAATGATACTGAGATTCTTGATTGGATTGAGGATGAAGGTAGCGACGGAGAAGCTGAATTCGATGGTGATGATGATGATGATGAGAATGATGAAGAAGAGGAAGAGGAAGAGGAGGAGGAGATTGATGATTTTGATGTTAACGAAGACCAAAATCTAGTTACGGACATGGAAGAAAGCGAAGATGGTAAATTTCACGCGATGGACGAAGACGAGAAGGACTGGAAGGAGTTTCGAAAATCAACAAATAGTGCCGAGGCAATGGAAAACATGGCAAGAAAGAGTGTCGAGCTCTCTACAAAGTTATACAAGAAGCAGATGATGACGGCGGAGAAGGCAGAGAAAGAAACAATGCACGATTCAGTAGATGGAGAGGAAACCGCCTTGAGAGGAAAGCGAGCAAAGGTCGGTCCAGAAGAGTGGAAGTATGTTGGGCTCGGTCCGTGGAGAAAAAAGATCAAGAAGAGCAAACTTCCTCCGGACCAATTTCTGCGAGCAGCTATTCGGCCTTTCACTTACAGAAATCTTGTTAAAGAGATTGTTTTAACTAGGCATGCAATTTTGGATGGCGATATTGGTAAGAAAGAGTAAGAATATAGTAATGTTGAGAAAGTTAGTAGAATGAATCATTGTCCTTGCTTTTGTGTAAGGATTTTTTGTTATGCTTACCAAGTTTCTGAAAAAGATTATGACAACATACAACATCGATTTATGTATACANGTTCTTCTCTGTGTTTTCAAANGATTTTGATGTTAG
TARGET=1315,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1654
PRIMER_LEFT_PENALTY=0.163086
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=GAATTCGATGGTGATGATGATG
PRIMER_RIGHT_SEQUENCE=TGTTTCTTTCTCTGCCTTCTCC
PRIMER_LEFT=1260,22
PRIMER_RIGHT=1553,22
PRIMER_LEFT_TM=60.163
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=0.1981
PRIMER_LEFT_1_PENALTY=0.102638
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=AGAAGCTGAATTCGATGGTGAT
PRIMER_RIGHT_1_SEQUENCE=TACCATCTTCGCTTTCTTCCAT
PRIMER_LEFT_1=1253,22
PRIMER_RIGHT_1=1392,22
PRIMER_LEFT_1_TM=60.103
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=140
PRIMER_PAIR_PENALTY_2=0.2345
PRIMER_LEFT_2_PENALTY=0.139083
PRIMER_RIGHT_2_PENALTY=0.095445
PRIMER_LEFT_2_SEQUENCE=AGAGATGGAGCTTGGTTGTGAT
PRIMER_RIGHT_2_SEQUENCE=TACCATCTTCGCTTTCTTCCAT
PRIMER_LEFT_2=1148,22
PRIMER_RIGHT_2=1392,22
PRIMER_LEFT_2_TM=60.139
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.2585
PRIMER_LEFT_3_PENALTY=0.163086
PRIMER_RIGHT_3_PENALTY=0.095445
PRIMER_LEFT_3_SEQUENCE=GAATTCGATGGTGATGATGATG
PRIMER_RIGHT_3_SEQUENCE=TACCATCTTCGCTTTCTTCCAT
PRIMER_LEFT_3=1260,22
PRIMER_RIGHT_3=1392,22
PRIMER_LEFT_3_TM=60.163
PRIMER_RIGHT_3_TM=60.095
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=0.2648
PRIMER_LEFT_4_PENALTY=0.163086
PRIMER_RIGHT_4_PENALTY=0.101680
PRIMER_LEFT_4_SEQUENCE=GAATTCGATGGTGATGATGATG
PRIMER_RIGHT_4_SEQUENCE=GCATTGTTTCTTTCTCTGCCTT
PRIMER_LEFT_4=1260,22
PRIMER_RIGHT_4=1557,22
PRIMER_LEFT_4_TM=60.163
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_04661 Ps_contig_mira-and-tgicl-ass_franssen_34196
SEQUENCE=TCTTCTCCCCTTTGAAATTCTATTCTTCTTCTTCTCCTTTTCTTAATTCTTCTATTACAATGCAAACTCCAAACCCTGATTCTTTGGGTATTAAGAAAGACATGAATAAAAAAGCTTCATCTTCTTCAACACCCACCAAATATGATAGTTCCTCCGGTACACCGGATTCTAGTGAAGATGCTTCGAATGGTGTTACGATTGATGATTTACATCATTTATATAAGCTTGTGGAGGAGAAAGATGGAGGTCTTCCTTGGATACATATGATGGATCGGTCTACTCCGACTTTGAGATACCAGGCGTGGCGTAGAGAACCAAAGGATGGACCTCCCCAATATCGAAGCAGTACTATTTTTGAGGATGCAACACCTGAGATAGTGAGGGATTTATTCTGGGACGATCAGTTTCGACCTAAGTGGGATGATATGCTTATTAACTCGACGACATTAGAAGAGTGCCCTACTACTGGAACAATGAAAGTGCATTGGGTTCGAAAGTTCCCCTTCTTCTGTAAAGACAGAGACTATGTTATCGGACGAAGAATATGGGAATGTGGGAGGTCATACTATTGTGTTACAAAGGGGGTAGATTGTCCTTCAATCCCAAGACAAGACAAACCCAGACGTGTCGATGTGTATTACTCTAGCTGGTGCATTCGAGCAGTTGAATCAAAGAGAGGTAATGGTCAATTGACTGCATGTGAAGTTTTACTCTTCCATCATGAAGAAATGGGAATTCCATGGGAGCTTGCAAAGCTTGGAGTAAGAAAAGGTATGTGGGGAACGGTACAGAAGGTTGAGCCTGGTTTGCGTGCTTATCAAGAAGCAAAAGCTTCTGGAGCTCCACTCTCTCGTTCCGCCTTCATGGCCAGTGTCAACACAAAAATAAGTCCGGAGTACTTGCAATCCATCGGATCCTCTGACGATTCATCACAAATCGACAGTGCAATCACTTCTGACAAACCAAAGGGCGTGAACGTACCGAAGATGCTAGTCATTGGCGGTGCTGTTGCTCTTGCTTGTAGTCTTGATAAGGGGTTGTTGACCAAGTATCTTTTATTTGGTGTTGCTAGAAGATTTGCTAATATGGGTAAAAGATAGTAGAACTAAAGGTGGAACCAATCTCTTGAAGGGATTCAGGTGTGTTGCAGTCACAAATCCATGCAGTCAGAGATCACTCGGTGCAGAAAGAAAGTATTAGCTGTCACTGCTGTGCTGATTGTGTGGGATTTGTGACTCTTGAAGCCATTGTTTTGGATATTCCACATTATCCGTTATAGTTTCCTTCTTATTATCACCTATAAATTAGGAGGCTCTTTTTTGGGTAAATAATCTATAAAAAAATAAAATCCAGGGGAAATGAGAAAATCTCGGCTTGTTGATTTGGGAGAAAGGCTAGTTGATAATTTTTCAAAACTTTTTATTTATTTCTTAGACCATGAGAGTTGGTGAAGACCACAGAATTTGTAAACTTTGTATGGTTGTATAAACTTCTTAGCAATAAAGAATAAAGATAATCTATTTTATA
TARGET=23,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04662 Ps_contig_mira-and-tgicl-ass_franssen_34197
SEQUENCE=ACATTGCTGAAGCCANATGTGGATGGAAGGGGAAAGGGTCAGAGCATGCGCCTGAGATCAAACAACGTCTGCACATCCATTAATGATCATTGGAAATGGTGACCAATGATTAAAGGATAACATTCCCGTTTTAAAAGGATAAACAAAAAACGGATTAAATTAAACCATGAACGCGTCTCTCTCTCACTTCATCTCCCGTTTCCATCAAACCTTACTCTCCGCCGTACCAGAATTCAACGCTGGCGGTGGCGGAGACTTAAAACTCTCCAAACTTACATCACTCAAACTGTCTCCCTCCCCTCATCCCAAATCTACCTTCCGTTTTCCATAATTCCCCCTTTAACTCCTTAGTCACACTAATCTATCCAAGAACCCTAGTAGACTAAAACCTAAATTAAATCCCTAAACAAAGGAATCAAAACCTATAGACTTAGGGCTTTGATTCTTTATCTCAAAAATCACATAGCGGTAACAAGATATGGAGGTAAATAGAGGTAAAAATGAACCTACCTTTGGAGGATGACGAATTCCGGTGTTTCTTACAGCGGGGATGATGAATGATGATCAAAGATGCGTTCGGGGTTAGTAAAGTTTGGTTTTGCAGATAGAATAGAAAATTATTAANAGCTGCTGTAGTTATTAGG
TARGET=176,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4081
PRIMER_LEFT_PENALTY=0.332305
PRIMER_RIGHT_PENALTY=0.075753
PRIMER_LEFT_SEQUENCE=AGATCAAACAACGTCTGCACAT
PRIMER_RIGHT_SEQUENCE=AAACGGAAGGTAGATTTGGGAT
PRIMER_LEFT=54,22
PRIMER_RIGHT=323,22
PRIMER_LEFT_TM=59.668
PRIMER_RIGHT_TM=60.076
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=270
PRIMER_PAIR_PENALTY_1=0.4153
PRIMER_LEFT_1_PENALTY=0.332305
PRIMER_RIGHT_1_PENALTY=0.082963
PRIMER_LEFT_1_SEQUENCE=AGATCAAACAACGTCTGCACAT
PRIMER_RIGHT_1_SEQUENCE=GGAGTTAAAGGGGGAATTATGG
PRIMER_LEFT_1=54,22
PRIMER_RIGHT_1=346,22
PRIMER_LEFT_1_TM=59.668
PRIMER_RIGHT_1_TM=59.917
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.4609
PRIMER_LEFT_2_PENALTY=0.332305
PRIMER_RIGHT_2_PENALTY=0.128591
PRIMER_LEFT_2_SEQUENCE=AGATCAAACAACGTCTGCACAT
PRIMER_RIGHT_2_SEQUENCE=GAGTAAGGTTTGATGGAAACGG
PRIMER_LEFT_2=54,22
PRIMER_RIGHT_2=216,22
PRIMER_LEFT_2_TM=59.668
PRIMER_RIGHT_2_TM=59.871
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=0.5040
PRIMER_LEFT_3_PENALTY=0.428205
PRIMER_RIGHT_3_PENALTY=0.075753
PRIMER_LEFT_3_SEQUENCE=AACAACGTCTGCACATCCATTA
PRIMER_RIGHT_3_SEQUENCE=AAACGGAAGGTAGATTTGGGAT
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=323,22
PRIMER_LEFT_3_TM=60.428
PRIMER_RIGHT_3_TM=60.076
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=0.6445
PRIMER_LEFT_4_PENALTY=0.568731
PRIMER_RIGHT_4_PENALTY=0.075753
PRIMER_LEFT_4_SEQUENCE=GATCAAACAACGTCTGCACATC
PRIMER_RIGHT_4_SEQUENCE=AAACGGAAGGTAGATTTGGGAT
PRIMER_LEFT_4=55,22
PRIMER_RIGHT_4=323,22
PRIMER_LEFT_4_TM=60.569
PRIMER_RIGHT_4_TM=60.076
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_04663 Ps_contig_mira-and-tgicl-ass_franssen_34198
SEQUENCE=TGTTCCGTTGGCTTCGAGTTTTTTCCGGTGGAGATGAAGATGAATTGAAGGAGCTTGGTAGTTCGTTTTCGTATTCGCCGAGTAAGTTTGGTGGATCTTGGAAGAGGGATTATCAGAATCAGATTCAGAGTCCGGTTTCTGTTTTTCAGTGTCCGGTTTCGTGTGGTAGTAGTATGGGGACAGTTCGTTCGTCGAGGAGTAGTGCTGGTGGGTTCTTTGAAGGGTTTGTGAGGAGAGCTTTGGGTTCTTCTTGTTTGGAGTATGATTCTACTGGTGTTAGGCTTCGCGGTGGTGGTGGTGGAGGTGGTGAGTTTGATGGAGGTTCTTCTTCTGCACTTGTTGATGAGTTGACTTTCAATTTGGAGGATACTTTTGTAGAGGGCTGCTTTGGATTTGAGTTTGAGCCTTATGCTAAGAAGTTGTTGATGAGTGCTCAGTTGAGGCACAAGATCTTTTGTGAAGAGTTTGTTATTAAGGCGTTTTGTGAAGCTGAGAAAGCACATAGAGGACAGATGCGGGCTAGCGGTGATCCGTATTTGCAGCATTGTTTGGAAACTGCTGTGTTGCTGGCTTTGATCGGTGCAAATTCRACTGTAGTTGCTGCAGGGCGTTTGCATGATACGCTTGATGATGCTTTTCTCACTTATGATTATATATTTGGGATGTTTGGAGCTGGAGTTGCTGATTTAGTTGAAGGGGTTTCTAAACTGAGTCACTTGAGTAAGTTGGCTCGAGACAACAACACAGCTAGTAAGTCTGTTGAAGCAAGACCGCCTGCATACAATGTTCCTTG
TARGET=288,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04664 Ps_contig_mira-and-tgicl-ass_franssen_34202
SEQUENCE=TTCCGAYGTTCRGAYCGAAGAAGTTTCCGACGTTCAGGTCGATGCGACTTGTGGCATTCAGGCCAATTTTCCGATGTTCAGATCGAAGTCCTTTCCAGTATTCAGACTGATGAGCGGCATTCAGGCCATGGTTATTTCTGTGTTACCATTTATTTTGGTATCCAGTTTAACATTCTTTTTCGATATTCAGACTGACTCTCACCGTACCAGACGGATTCTTCTTTCAAGACCACCTCTTTGCCGATTCTGACAGACATTGTTACTTCACTTCACTTCAGTGCAAATTTTTGGGCTTTTATTGTATTCAATCCCTTGATACCTCGAAAGCACGAAAGCCGCTGCTATCTTCTTTCCAGGTCTCCAGTTGATTGAATAGGGGCAGCTGTAATACCCCAAAATTTACCCTTCATTTTTCCTRGAAGCATACGCTTTACACCTCATGCATGCATTCTATTTTTAGGTCATTTAGCTATTTTTATTTCATCATGGCAATACGGTAATTATTTTTTAAC
TARGET=262,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04665 Ps_contig_mira-and-tgicl-ass_franssen_34211
SEQUENCE=TGTCTNGCTGTTTCGTTTTCTTCGTCTTTTCAGTTAACTGCAGAACCACTCCGATTCATTCATTCCTCGATCTATATCAATCTACTTCGATTCCTTCGTTTTTCTTCAATTCGCATTTTCTTTCACTTTTTTCTCTGGGGTTTCGTTTTAGGAACAGTGTTATCGAAGATTTAGGGTTTTCGTTCATTCATCTTTCTCCTAGATCCGTCACCAGAGCTAGGAGAAACATTGCATGCTTGTTTTGCTTTAGAATTTAGCTATAGTAATGGCGTCTTCGTATCAGGGTTCCGTTAGTGCTGCCCAGGTGGGTTCTTACTTCGTTGGACAGTACTATCAAGTTCTTCGTCAGCAGCCTGCTCTTGTTCATCAGTTCTACTCCGATTTAAGCTCAATGATTCGTGTTGATGGTGATTATACCGAGAACGCATCTGATGTGTTGCAAATTCATAATGTTGTTACGTCGTTGAATTTTTCGGCAATTGAGATCAAGACAATCAATTCTCTGGACTCTTGGAATGGAGGAGTGGTTGTGATGGTTTCGGGTGTTGTTAAGATCAAGGATGTCAATAGGAGGCAGAGGTTTGTTCAAACATTCTTCCTTGCACCTCAGGAGAAGGGTTACTTTGTGCTCAATGATATATTTCAGTTTGTTGAGGATGAAGTAGTGCATCAGAATCTGATACCGGTGACATCTGAGAGGATTGAATCACAGCCACATGTTTCTGCTTCTTATGCTGAACCACCAGTTTCGGACTATGGGTTTGAGGAAGAAGCAAGAGAATATCTCAACTCAGTTCATATAGATGATGATCCAGTGGACAAGTATAGTCTTCCTGAGCAGCAGCAGCAACAGCAACTACAAGAAGATCTCGAGACTGAAGTTGTGGTGGAGGAAACTCCTACACAGGAATCATATCCACCGGTGCATGATGTTGCTCATACTATCCGTGAAACCCCTGTCACTCTTGTGGAAGAGTCTTTTGAGGAGCCTGCTAAGAAAACTTATGCATCTATTTTACGTGTCTCCAAAGGCCAATCAGTGTTGCCCGCTGCACCCGAACATGCCCCACATCATTCTTTTCAAAGTGCTCCTCCTTCTGAGTTTAATCATGTGACACAGCCTGCTGCTCAGCAGGCTGTGGTGCAGCCTGTTTATCAGCAGTCAAGCTCAGCATCTGCATATGTTTCAGAGTCAGGGGCCGATGCAGCAGTGGATGGCTACAGATTTGACCATGAAGAAGTAACATCTGTCTATGTGAGAAACCTGCCTGCTGATATTACCGAAGCTGAGATTGACCAGGAATTTAAGAATTTTGGCAGAATTAAGCCAGATGGAATATTCGTAAGGGTCAAGCAGGAAATTGGAGTTTGCTATGCATTTGTGGAATTCGAAGACGTTATTGGCGTTCAAAATGCACTTCAGGCTTCTCCAATACATATGGATGGAAGACCGATATACATTGAGGAGCGAAGACCAAGCACTAGCAGTGCAGCTCGAGGAGGAAGAGGAAGGGGAAGAGGCAGAGGCAGTTATCCAACAGATGCTCCGAGAGGGCGTTTTGGTGGTAGGAGCTCGGGCAGGGGATATTATCAGGACACTTCAGACTATACCAGACCCAGAGGCGACAGTTATGTTCAGTATGGTTCACGATAGGAAGATGTCGTGAAAGCGTGGTTGAATATGTATTTGAAGGGGCTTATACAATTAGTGAAATCTGAGCTTGATTCGGTATCTAGTGCACAACATTTTCCTTCTATGGTTTCATGAAAAGGATTCTTACATTTTGAGTGTCTTTAAAAGCTGTTGAGACTTAATAGTTTGTTTTGCGAGTATAGATCAGCTAGTTCCCTTTGAATGGTTTATATGATGCCATTCAGGTGGGAAGTTTTGTTATTTAAATTGCCTCATGTCAGGATCATGGATGTTTCTTTAATGAGAAAAGGTGTTGAAAGAAAGGTT
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3307
PRIMER_LEFT_PENALTY=0.066394
PRIMER_RIGHT_PENALTY=0.264300
PRIMER_LEFT_SEQUENCE=GCTGTTTCGTTTTCTTCGTCTT
PRIMER_RIGHT_SEQUENCE=CAAGCATGCAATGTTTCTCCTA
PRIMER_LEFT=6,22
PRIMER_RIGHT=238,22
PRIMER_LEFT_TM=59.934
PRIMER_RIGHT_TM=60.264
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.4121
PRIMER_LEFT_1_PENALTY=0.066394
PRIMER_RIGHT_1_PENALTY=0.345718
PRIMER_LEFT_1_SEQUENCE=GCTGTTTCGTTTTCTTCGTCTT
PRIMER_RIGHT_1_SEQUENCE=TGCAATGTTTCTCCTAGCTCTG
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=59.934
PRIMER_RIGHT_1_TM=59.654
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=227
PRIMER_PAIR_PENALTY_2=0.4590
PRIMER_LEFT_2_PENALTY=0.066394
PRIMER_RIGHT_2_PENALTY=0.392624
PRIMER_LEFT_2_SEQUENCE=GCTGTTTCGTTTTCTTCGTCTT
PRIMER_RIGHT_2_SEQUENCE=GCAATGTTTCTCCTAGCTCTGG
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=231,22
PRIMER_LEFT_2_TM=59.934
PRIMER_RIGHT_2_TM=60.393
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.5021
PRIMER_LEFT_3_PENALTY=0.066394
PRIMER_RIGHT_3_PENALTY=0.435722
PRIMER_LEFT_3_SEQUENCE=GCTGTTTCGTTTTCTTCGTCTT
PRIMER_RIGHT_3_SEQUENCE=GTTTCTCCTAGCTCTGGTGACG
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=226,22
PRIMER_LEFT_3_TM=59.934
PRIMER_RIGHT_3_TM=60.436
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.5184
PRIMER_LEFT_4_PENALTY=0.066394
PRIMER_RIGHT_4_PENALTY=0.452017
PRIMER_LEFT_4_SEQUENCE=GCTGTTTCGTTTTCTTCGTCTT
PRIMER_RIGHT_4_SEQUENCE=AGCACTAACGGAACCCTGATAC
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=59.934
PRIMER_RIGHT_4_TM=59.548
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_04666 Ps_contig_mira-and-tgicl-ass_franssen_34211
SEQUENCE=TGTCTNGCTGTTTCGTTTTCTTCGTCTTTTCAGTTAACTGCAGAACCACTCCGATTCATTCATTCCTCGATCTATATCAATCTACTTCGATTCCTTCGTTTTTCTTCAATTCGCATTTTCTTTCACTTTTTTCTCTGGGGTTTCGTTTTAGGAACAGTGTTATCGAAGATTTAGGGTTTTCGTTCATTCATCTTTCTCCTAGATCCGTCACCAGAGCTAGGAGAAACATTGCATGCTTGTTTTGCTTTAGAATTTAGCTATAGTAATGGCGTCTTCGTATCAGGGTTCCGTTAGTGCTGCCCAGGTGGGTTCTTACTTCGTTGGACAGTACTATCAAGTTCTTCGTCAGCAGCCTGCTCTTGTTCATCAGTTCTACTCCGATTTAAGCTCAATGATTCGTGTTGATGGTGATTATACCGAGAACGCATCTGATGTGTTGCAAATTCATAATGTTGTTACGTCGTTGAATTTTTCGGCAATTGAGATCAAGACAATCAATTCTCTGGACTCTTGGAATGGAGGAGTGGTTGTGATGGTTTCGGGTGTTGTTAAGATCAAGGATGTCAATAGGAGGCAGAGGTTTGTTCAAACATTCTTCCTTGCACCTCAGGAGAAGGGTTACTTTGTGCTCAATGATATATTTCAGTTTGTTGAGGATGAAGTAGTGCATCAGAATCTGATACCGGTGACATCTGAGAGGATTGAATCACAGCCACATGTTTCTGCTTCTTATGCTGAACCACCAGTTTCGGACTATGGGTTTGAGGAAGAAGCAAGAGAATATCTCAACTCAGTTCATATAGATGATGATCCAGTGGACAAGTATAGTCTTCCTGAGCAGCAGCAGCAACAGCAACTACAAGAAGATCTCGAGACTGAAGTTGTGGTGGAGGAAACTCCTACACAGGAATCATATCCACCGGTGCATGATGTTGCTCATACTATCCGTGAAACCCCTGTCACTCTTGTGGAAGAGTCTTTTGAGGAGCCTGCTAAGAAAACTTATGCATCTATTTTACGTGTCTCCAAAGGCCAATCAGTGTTGCCCGCTGCACCCGAACATGCCCCACATCATTCTTTTCAAAGTGCTCCTCCTTCTGAGTTTAATCATGTGACACAGCCTGCTGCTCAGCAGGCTGTGGTGCAGCCTGTTTATCAGCAGTCAAGCTCAGCATCTGCATATGTTTCAGAGTCAGGGGCCGATGCAGCAGTGGATGGCTACAGATTTGACCATGAAGAAGTAACATCTGTCTATGTGAGAAACCTGCCTGCTGATATTACCGAAGCTGAGATTGACCAGGAATTTAAGAATTTTGGCAGAATTAAGCCAGATGGAATATTCGTAAGGGTCAAGCAGGAAATTGGAGTTTGCTATGCATTTGTGGAATTCGAAGACGTTATTGGCGTTCAAAATGCACTTCAGGCTTCTCCAATACATATGGATGGAAGACCGATATACATTGAGGAGCGAAGACCAAGCACTAGCAGTGCAGCTCGAGGAGGAAGAGGAAGGGGAAGAGGCAGAGGCAGTTATCCAACAGATGCTCCGAGAGGGCGTTTTGGTGGTAGGAGCTCGGGCAGGGGATATTATCAGGACACTTCAGACTATACCAGACCCAGAGGCGACAGTTATGTTCAGTATGGTTCACGATAGGAAGATGTCGTGAAAGCGTGGTTGAATATGTATTTGAAGGGGCTTATACAATTAGTGAAATCTGAGCTTGATTCGGTATCTAGTGCACAACATTTTCCTTCTATGGTTTCATGAAAAGGATTCTTACATTTTGAGTGTCTTTAAAAGCTGTTGAGACTTAATAGTTTGTTTTGCGAGTATAGATCAGCTAGTTCCCTTTGAATGGTTTATATGATGCCATTCAGGTGGGAAGTTTTGTTATTTAAATTGCCTCATGTCAGGATCATGGATGTTTCTTTAATGAGAAAAGGTGTTGAAAGAAAGGTT
TARGET=837,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0186
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.016312
PRIMER_LEFT_SEQUENCE=GGTTTGAGGAAGAAGCAAGAGA
PRIMER_RIGHT_SEQUENCE=GAGTTTCCTCCACCACAACTTC
PRIMER_LEFT=758,22
PRIMER_RIGHT=898,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.016
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.0317
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.029407
PRIMER_LEFT_1_SEQUENCE=GGTTTGAGGAAGAAGCAAGAGA
PRIMER_RIGHT_1_SEQUENCE=AGTATGAGCAACATCATGCACC
PRIMER_LEFT_1=758,22
PRIMER_RIGHT_1=942,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.029
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.0342
PRIMER_LEFT_2_PENALTY=0.017846
PRIMER_RIGHT_2_PENALTY=0.016312
PRIMER_LEFT_2_SEQUENCE=TTCTGCTTCTTATGCTGAACCA
PRIMER_RIGHT_2_SEQUENCE=GAGTTTCCTCCACCACAACTTC
PRIMER_LEFT_2=720,22
PRIMER_RIGHT_2=898,22
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=60.016
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.0473
PRIMER_LEFT_3_PENALTY=0.017846
PRIMER_RIGHT_3_PENALTY=0.029407
PRIMER_LEFT_3_SEQUENCE=TTCTGCTTCTTATGCTGAACCA
PRIMER_RIGHT_3_SEQUENCE=AGTATGAGCAACATCATGCACC
PRIMER_LEFT_3=720,22
PRIMER_RIGHT_3=942,22
PRIMER_LEFT_3_TM=60.018
PRIMER_RIGHT_3_TM=60.029
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.1399
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.137550
PRIMER_LEFT_4_SEQUENCE=GGTTTGAGGAAGAAGCAAGAGA
PRIMER_RIGHT_4_SEQUENCE=GGGTTTCACGGATAGTATGAGC
PRIMER_LEFT_4=758,22
PRIMER_RIGHT_4=955,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=59.862
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=198
=
PRIMER_SEQUENCE_ID=SSR_04667 Ps_contig_mira-and-tgicl-ass_franssen_34212
SEQUENCE=TCAAAATTAAAAATCTTAAAGTACTAAATCCGAAGAATAGTTGGAAATTTGATTNGGAGTGAGAAAAAAGTGAAGGGTGGCAGAGGGAGAAAGAGTGATGTCGACCAGTGACAGAAGCCAAGTATCAAAGCTCAGCAAAAGCCCTAAAGATGGTGAACGGATCATCGCTTCGACCCGTCGACCCGACGGAACACTCCGAAAAGAAATTCGAATCCGACCCGGTTACGTTCCTCCAGACGAAGTCGCTATCTATCAACCAAAACCCGCACTGATGAGAAAAGAAATGGCTTCACATATTGGTCCTCCTCCAGGTTATGACCCTCAATTGGATTCAAAACCGAAAACCAAAGCTGTTAAGAGAAATGAAAGAAAGAAAGAAAAGAAACGACTTCAGGTAAAGGAAACTAACTTAGAACCAACTCTAGTTGAAGATAGTATAAAGCAAGAAGCGGTAGTTGTGGAGAATGCTGTACCATTCATTAACTTCTCAGATCAATGAA
TARGET=365,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0318
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=GCTTCACATATTGGTCCTCCTC
PRIMER_RIGHT_SEQUENCE=GAATGGTACAGCATTCTCCACA
PRIMER_LEFT=286,22
PRIMER_RIGHT=477,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=0.0818
PRIMER_LEFT_1_PENALTY=0.081801
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=AAATGGCTTCACATATTGGTCC
PRIMER_RIGHT_1_SEQUENCE=GAATGGTACAGCATTCTCCACA
PRIMER_LEFT_1=281,22
PRIMER_RIGHT_1=477,22
PRIMER_LEFT_1_TM=60.082
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=0.1090
PRIMER_LEFT_2_PENALTY=0.108929
PRIMER_RIGHT_2_PENALTY=0.000037
PRIMER_LEFT_2_SEQUENCE=GAACACTCCGAAAAGAAATTCG
PRIMER_RIGHT_2_SEQUENCE=GAATGGTACAGCATTCTCCACA
PRIMER_LEFT_2=188,22
PRIMER_RIGHT_2=477,22
PRIMER_LEFT_2_TM=60.109
PRIMER_RIGHT_2_TM=60.000
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.1173
PRIMER_LEFT_3_PENALTY=0.117281
PRIMER_RIGHT_3_PENALTY=0.000037
PRIMER_LEFT_3_SEQUENCE=CGCACTGATGAGAAAAGAAATG
PRIMER_RIGHT_3_SEQUENCE=GAATGGTACAGCATTCTCCACA
PRIMER_LEFT_3=264,22
PRIMER_RIGHT_3=477,22
PRIMER_LEFT_3_TM=59.883
PRIMER_RIGHT_3_TM=60.000
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.1361
PRIMER_LEFT_4_PENALTY=0.136083
PRIMER_RIGHT_4_PENALTY=0.000037
PRIMER_LEFT_4_SEQUENCE=CGCTATCTATCAACCAAAACCC
PRIMER_RIGHT_4_SEQUENCE=GAATGGTACAGCATTCTCCACA
PRIMER_LEFT_4=243,22
PRIMER_RIGHT_4=477,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=60.000
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_04668 Ps_contig_mira-and-tgicl-ass_franssen_34216
SEQUENCE=CCAGGTTCTTCCCTAATAAAAATAGGCTCTATGAAAGATAAATTCTTACCTACCCATAAGTTTTTATTGGTATCAGCACTTTTTACGCAAAATGTATTTAAATTTCTAATTTATTTTAAAGTTAATTATTATAAAAAAGTGACATAATACTTTATTATATTAGATACCTAAATTTTAGAGTGTTCATTTTTATAAAATTTATTTTCTTTAAAAGTAGATAAAAAAGTATAATATACACCAACAATTTTAAAATTGTCAATAATTATTGAAGAAAGTGTTTTCTTAACAATTCACACATTTACTTATAAAATGCCAAATATGATATGGTAAATATAAATTTTGATTGCTTATATGTATAATATTGTATTTATAATGTGAATGCATTATCTTTATATTTATTAAATGTAAAATATTCTTATCTAAAAGTGGATTAACAACGAATTTCAACATAAAATTATATCCAAATTCAAAGACTATAAATTTTAATATTAAATTAAAATTAATTTTAAAAACATAATTAATCATTTTAAAATAGAATGTAAAATATTTCATCTTCATTAATCATTATAAAAAGAAAAGAAAAAAGATACATGAATGACAAGCAAGACACACCAAAACCACAAACTCAACCTAACCCACCTAACCAACCAAAAGCTCTCTATAAAAACCCAACAACNACTACCAACATTTCGGATTACACCACAACACAAAAATGGATCTCCTTCTTCTCGAAAAGACCCTTTTAGCCCTCTTCCTCGCCGCCATAACCGCCATCACAATCTCAAAACTCCGCGGCAAACGCTTCAAACTCCCTCCTGGCCCCTTCCCCGTCCCCGTCTTCGGCAACTGGCTCCAAGTCGGCGATGATCTCAACCACCGCAACCTCACCGATTTAGCCAAACGCTTCGGCGAAATCCTCCTCCTCCGCATGGGACAACGCAACCTCGTCGTCATCTCCTCCCCGGAACTCGCCAAAGAAGTTCTCCATACCCAAGGAGTCGAGTTCGGCTCCCGAACACGAAACGTCGTCTTCGACATCTTCACCGGAAAAGGCCAGGACATGGTTTTCACTGTCTACGGCGAACACTGGCGGAAAATGCGAAGAATCATGACCGTCCCGTTCTTCACCAACAAAGTTGTTCAACAGTACCGATTCGGTTGGGAATCGGAAGCCGCTAGTGTTGTTGATGATGTGAAGAAAAATTCCAAAGCTAGTGTGAATGGAATTGTGATTCGGAGAAGATTGCAATTGATGATGTATAATATCATGTATAGGATTATGTTTGATAGAAGATTTGAGAGCGAGGAAGATCCTTTGTTTGTGAAGTTGAAAGCTTTGAACGGTGAAAGGAGTCGTTTGGCGCAAAGCTTTGAGTATAACTATGGCGATTTTATTCCAATTTTGAGACCTTTTTTGAAGGGTTATTTGAAGGTTTGTAAAGAGGTTAAAGATAGAAGGTTGCAGCTTTTCAAAGACTACTTTGTTGATGAAAGAAAGAAGCTTGGAAGTACTAAGAGCACATATAATGAAGGATTAAAATGTGCTATTGATCATATTTTGGATGCTCAAAAGAAGGGTGAGATCAATGATGACAATGTGCTTTATATTGTTGAGAACATCAATGTTGCTGCAATTGAGACAACATTATGGTCAATTGAATGGGGAATTGCTGAGCTAGTGAACCATCAAGAGATTCAAAACAAGTTAAGGGAAGAGATGGACAAAGTTCTTGGACCAGGACACCAAGTAACCGAACCAGATCTCGAGAAGCTACCTTACCTACAAGCCGTGATCAAAGAGACCCTCCGTCTTCGAATGGCGATTCCACTTCTCGTACCACACATGAACCTTCATGATGCCAAGCTCGGTGGTTTTGACATCCCGGCTGAAAGCAAGATACTGGTCAATGCGTGGTGGCTTGCAAACAACCCGGCTCTATGGAAAAAGCCGGAGGAGTTTAGGCCCGAGAGGTTCTTGGAGGAAGAGGCTCATGTTGAGGCGAACGGGAATGATTTTAGGTACCTTCCGTTCGGTGTTGGTAGAAGGAGTTGCCCTGGGATTATTCTCGCTTTACCGATCCTTGGTATTACTATCGGTCGTCTGGTCCAGAATTTCGAGCTATTGCCTCCACCGGGACAATCTAAGATCGACACTTCTGAGAAAGGAGGACAATTTAGTCTGCACATACTCAAACATTCCACCATTGTTGCTAAGCCAAGAGCATTTTAATTAGGCTTCATTCACATTAATTCCCTTTTATAGTTTGTTTTTCTTTACTTTGTGCAATGCATTTTAATGATCATGATGTGGGAATGTTATTAAAATGTCTTATTAGCCTACAATGGTAGGTGAATAATGTTGTTGAGTTGTGTTTGTCACATGTATAAATCTTTTGAAGTTGAAGTTATGGTTTTGGGATGATTTTGTAACAACACTTGTCTCTTGGTAATAACTTTTGGTAAACTANNATGATTTATGAACT
TARGET=915,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0449
PRIMER_LEFT_PENALTY=0.700378
PRIMER_RIGHT_PENALTY=0.344527
PRIMER_LEFT_SEQUENCE=AAAGACCCTTTTAGCCCTCTTC
PRIMER_RIGHT_SEQUENCE=TGGGTATGGAGAACTTCTTTGG
PRIMER_LEFT=732,22
PRIMER_RIGHT=992,22
PRIMER_LEFT_TM=59.300
PRIMER_RIGHT_TM=60.345
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=1.0449
PRIMER_LEFT_1_PENALTY=0.700378
PRIMER_RIGHT_1_PENALTY=0.344527
PRIMER_LEFT_1_SEQUENCE=GAAAAGACCCTTTTAGCCCTCT
PRIMER_RIGHT_1_SEQUENCE=TGGGTATGGAGAACTTCTTTGG
PRIMER_LEFT_1=730,22
PRIMER_RIGHT_1=992,22
PRIMER_LEFT_1_TM=59.300
PRIMER_RIGHT_1_TM=60.345
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=1.2101
PRIMER_LEFT_2_PENALTY=0.865622
PRIMER_RIGHT_2_PENALTY=0.344527
PRIMER_LEFT_2_SEQUENCE=GCCATCACAATCTCAAAACTCC
PRIMER_RIGHT_2_SEQUENCE=TGGGTATGGAGAACTTCTTTGG
PRIMER_LEFT_2=769,22
PRIMER_RIGHT_2=992,22
PRIMER_LEFT_2_TM=60.866
PRIMER_RIGHT_2_TM=60.345
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=1.2620
PRIMER_LEFT_3_PENALTY=0.917493
PRIMER_RIGHT_3_PENALTY=0.344527
PRIMER_LEFT_3_SEQUENCE=AGACCCTTTTAGCCCTCTTCCT
PRIMER_RIGHT_3_SEQUENCE=TGGGTATGGAGAACTTCTTTGG
PRIMER_LEFT_3=734,22
PRIMER_RIGHT_3=992,22
PRIMER_LEFT_3_TM=60.917
PRIMER_RIGHT_3_TM=60.345
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=1.2765
PRIMER_LEFT_4_PENALTY=0.664324
PRIMER_RIGHT_4_PENALTY=0.612170
PRIMER_LEFT_4_SEQUENCE=CCACAACACAAAAATGGATCTC
PRIMER_RIGHT_4_SEQUENCE=GTATGGAGAACTTCTTTGGCGA
PRIMER_LEFT_4=699,22
PRIMER_RIGHT_4=989,22
PRIMER_LEFT_4_TM=59.336
PRIMER_RIGHT_4_TM=60.612
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_04669 Ps_contig_mira-and-tgicl-ass_franssen_34219
SEQUENCE=AGTTTCTAAAACTATTTAACGGTGAGATTTATAACTACACAGTATGCAGTTAATAATCAATTCCCTGAGAGCCTGAATCCCATATCAATAGCCTAGTTGATGTGTTCTTGTAAGATGCAACGCCAACAAGGATATACAGAGCACTAACCAAAATAATGCAAAATATAAAACTAAATTAACTGTCCATCTGTTGTTGAATGAAAAACCGATTGCCGTTAATGAGGACGGCATCACAAAAAGTTGTACAAATGCACGTCTAGAATAAACCAAAATCAGCTTTCAATCATTGGTTCAGGACCAGATGTTTCCGTACGAGATTTGTTTGCTTGATGAACAGAGTTGAACAGAGTGTCCTTTATAACCTGTGACATTAAGCCATGCACTTGCTCCACCGTTATCTTCGCACTATCCCGAGTAATCTTAGCGAGTGGAGACTCTTCTTCTTTCTTTCTAGGTGTAGGTGCTACTAGTGGCCCGAAACGTGAGTGCGCCAATTGATTCTCTGCTTGTTCTAGCTTTTCAGCTAAATCCGATATTTGTCCAGCTACATAGTCTCCATTGCCCAATAAAGGAGAAGATGAGAGTGTATTCACCCAATATTTGTTCCACAGGAGATCCAAAAGGTGTGAATCAAGACTAGACTTAAAGTAAGTGATATCCAAAGAATAATACTGTTTACAATGGACACCAAAGTCTTCAATCTTATTAAGTGGTATAGTTTGATACTCAGAAATAGGATCATCGGGAGGCTTATAACCTTCCGGGTAAGTCCTGAAAGCCCCAATCTCAACTTTCCCAGCAGAAACAGTCCTTGTCGGATCAATCACAACCGCCAAAAATGGCTCCTGAAATTGCTGATTCAGCATCTGCGTCGAAACATCAATCCCAGAAAGCCAACAACCATAACCAGGATGAGAATGATACCATCCCACAACGTTTTCCAACCTTCCAGCCTGTTTATTGGTCTGAGAATAATCAACCATGTATTCATACGCATCAGCTTGAGCATTGACACGAGTTTCAGTACCTTCAACAGGAAGAGCGAAAGCATCCATAACGATGATTGAATCAGCATCAGTTTTACCTTGCATGAGACCCATAACCTCGATGGTACCCCCGGAGCGCGCGTGAACAACCATCTTGAGTAAAGCGAGTGCAGAGATCTTGACGCGCTTGAAGTAGTGAGGATCGTTTGCCCATGGTTTATCGCGTTGAAACTCGCTTTGTCCTGCTTCATCGTAGTGGAAAATTGAATCGTCGGCAGCCGGAGTTTCCATGGGGATTATGTTGTTCTCTAACTCCCACGTTTGTTGCGCAATCGCCGATGACGAAGAAGGTTCCATATTTGTTCTCTCCGACGTTCTCTCCGCCGTTGATCCTTTCTCTCCGGTGAGTGAGTGGGAGTTCGGAAGGCCACAACTCCGGAGCGACAGACTACTGAAGTGACGGTCCAGTCTTGAGGAGAGAGTNAAATAAGTTTAACATT
TARGET=441,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1208
PRIMER_LEFT_PENALTY=0.039178
PRIMER_RIGHT_PENALTY=0.081670
PRIMER_LEFT_SEQUENCE=GACGGCATCACAAAAAGTTGTA
PRIMER_RIGHT_SEQUENCE=GGGCCACTAGTAGCACCTACAC
PRIMER_LEFT=223,22
PRIMER_RIGHT=475,22
PRIMER_LEFT_TM=60.039
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=59.091
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.1240
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.008257
PRIMER_LEFT_1_SEQUENCE=TCGCACTATCCCGAGTAATCTT
PRIMER_RIGHT_1_SEQUENCE=TTGAAGACTTTGGTGTCCATTG
PRIMER_LEFT_1=400,22
PRIMER_RIGHT_1=699,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=60.008
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.2789
PRIMER_LEFT_2_PENALTY=0.197222
PRIMER_RIGHT_2_PENALTY=0.081670
PRIMER_LEFT_2_SEQUENCE=CTGTGACATTAAGCCATGCACT
PRIMER_RIGHT_2_SEQUENCE=GGCCACTAGTAGCACCTACACC
PRIMER_LEFT_2=362,22
PRIMER_RIGHT_2=474,22
PRIMER_LEFT_2_TM=60.197
PRIMER_RIGHT_2_TM=60.082
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=59.091
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=0.2789
PRIMER_LEFT_3_PENALTY=0.197222
PRIMER_RIGHT_3_PENALTY=0.081670
PRIMER_LEFT_3_SEQUENCE=CTGTGACATTAAGCCATGCACT
PRIMER_RIGHT_3_SEQUENCE=GGGCCACTAGTAGCACCTACAC
PRIMER_LEFT_3=362,22
PRIMER_RIGHT_3=475,22
PRIMER_LEFT_3_TM=60.197
PRIMER_RIGHT_3_TM=60.082
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=59.091
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=0.3352
PRIMER_LEFT_4_PENALTY=0.253482
PRIMER_RIGHT_4_PENALTY=0.081670
PRIMER_LEFT_4_SEQUENCE=CAGCTTTCAATCATTGGTTCAG
PRIMER_RIGHT_4_SEQUENCE=GGCCACTAGTAGCACCTACACC
PRIMER_LEFT_4=273,22
PRIMER_RIGHT_4=474,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=60.082
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=59.091
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_04670 Ps_contig_mira-and-tgicl-ass_franssen_34220
SEQUENCE=ATTATGNGCTCGGAGGAAAAAGCTGGTATACTTGTTACATCTATTGGTTTCATACATTTTCTAGAATCTCTTATGCGAAGTTCACAAGTTCACAACTTCACAACTTCACAAGTACTTCCTTTTTTATTGTATTTGCTACATATAAATGAAAAAGGTAGGGAAAAAATGAGTTACCTAAGCTACATAACATAACATAAATTACAAAACGCCGCATCACACTATAACCATTTCCTTCAAGACCTGAAGCTCGCTGAGACTTTAAATATTGCACACGGTTGGAATGATGAACTCAGATAAGCCAATAATAATGTATACTCAAGTTTTGTATTCACTGACGCGGATCCATTTGGTACTTGACCACTTATTCCCTTTGATCACTGGACAACCACCATGCAAGCTTGACGCATCTAAAGTTGCATCCGGCTTCATGCTCCAAAAAAGCAAAGCATCGCCTCTCTTTGGCTTAATTGAAAGTCCTTTTTTCCCACAATCAGAAAGCTCGTTATACCATGGCACAGAACTAAAATTGCCCTTGGCAGCAGGGAACACTGTCTCGCCCCCTTCTTCAACATCTGTAAGGTACATCAATACTGTTGCTATGCGCTGACCACCATTTTCTGTGTTAAACTCATCAAGAAAGTAGTCATAGTGAGGCTCATACTTTTGTCCAACTTCATAATGTAGAACTTGAAGTCCTTCACCGTGCTCTACAGGAATAAAAGTAAAGTCGGAAATTCGTTTCTCAATATCTCTGACAATCTTATCGCGTCCCCTGGCCAGAAAAGTTCCAGAGCTTGTACGCACTCTGCTATCTTTGCTCTTTCCAGTCTCACTGTCGACAACAGTTGACTTGTGCATACTTGGCTTTGCTATGTCAATCAGATATTCACATTCCTCCTTGGTCAGAAAATTGTGATAGACAAAAGCTCTAGGCTCCCATGATACAACTTCAACCCAGCGCTCTCCCTTACCATCATCATCATCAACACTATCGACGGTGTTATGAGCAATGGAGGTGAGATCGTTGGGTTTAGGCAAGTTTTGATTGGAAGAAGGAATGGAAAGGATTCCGAAAACGAGGAGAATGAGGATAAGGAAAGTGAAAGCGATGAAGAGAGCGAAGATGATAGTGTAAGAAGAAGATGATTTTCTAGATGGAAGACGAGAGTATCTGGATTTGGCCATTGTTGAGTTTTCAGATCTACGAGATCTGAAATTCAACGAATGTAAAAACAAGTTGGAATTGAATAAAAGAGAGAACAAGAGATAATGCTGTTACGACTTGTTAGATGGTGAGAGTAGAACGAAGGAAAGAAAGAAAGAAGGAACNAGGAAATGATTTTTTATATTTTCTTTTATTTTCTATT
TARGET=182,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1981
PRIMER_LEFT_PENALTY=0.174509
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=GCGAAGTTCACAAGTTCACAAC
PRIMER_RIGHT_SEQUENCE=TTCAGGTCTTGAAGGAAATGGT
PRIMER_LEFT=74,22
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=59.825
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=0.1981
PRIMER_LEFT_1_PENALTY=0.174509
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=GCGAAGTTCACAAGTTCACAAC
PRIMER_RIGHT_1_SEQUENCE=TCAGGTCTTGAAGGAAATGGTT
PRIMER_LEFT_1=74,22
PRIMER_RIGHT_1=243,22
PRIMER_LEFT_1_TM=59.825
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=1.0563
PRIMER_LEFT_2_PENALTY=1.032699
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=CGGAGGAAAAAGCTGGTATACTT
PRIMER_RIGHT_2_SEQUENCE=TCAGGTCTTGAAGGAAATGGTT
PRIMER_LEFT_2=10,23
PRIMER_RIGHT_2=243,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=234
PRIMER_PAIR_PENALTY_3=1.0563
PRIMER_LEFT_3_PENALTY=1.032699
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=CGGAGGAAAAAGCTGGTATACTT
PRIMER_RIGHT_3_SEQUENCE=TTCAGGTCTTGAAGGAAATGGT
PRIMER_LEFT_3=10,23
PRIMER_RIGHT_3=244,22
PRIMER_LEFT_3_TM=60.033
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=1.1873
PRIMER_LEFT_4_PENALTY=1.163717
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=ATTATGNGCTCGGAGGAAAAAG
PRIMER_RIGHT_4_SEQUENCE=TTCAGGTCTTGAAGGAAATGGT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=244,22
PRIMER_LEFT_4_TM=61.164
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.50
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_04671 Ps_contig_mira-and-tgicl-ass_franssen_34220
SEQUENCE=ATTATGNGCTCGGAGGAAAAAGCTGGTATACTTGTTACATCTATTGGTTTCATACATTTTCTAGAATCTCTTATGCGAAGTTCACAAGTTCACAACTTCACAACTTCACAAGTACTTCCTTTTTTATTGTATTTGCTACATATAAATGAAAAAGGTAGGGAAAAAATGAGTTACCTAAGCTACATAACATAACATAAATTACAAAACGCCGCATCACACTATAACCATTTCCTTCAAGACCTGAAGCTCGCTGAGACTTTAAATATTGCACACGGTTGGAATGATGAACTCAGATAAGCCAATAATAATGTATACTCAAGTTTTGTATTCACTGACGCGGATCCATTTGGTACTTGACCACTTATTCCCTTTGATCACTGGACAACCACCATGCAAGCTTGACGCATCTAAAGTTGCATCCGGCTTCATGCTCCAAAAAAGCAAAGCATCGCCTCTCTTTGGCTTAATTGAAAGTCCTTTTTTCCCACAATCAGAAAGCTCGTTATACCATGGCACAGAACTAAAATTGCCCTTGGCAGCAGGGAACACTGTCTCGCCCCCTTCTTCAACATCTGTAAGGTACATCAATACTGTTGCTATGCGCTGACCACCATTTTCTGTGTTAAACTCATCAAGAAAGTAGTCATAGTGAGGCTCATACTTTTGTCCAACTTCATAATGTAGAACTTGAAGTCCTTCACCGTGCTCTACAGGAATAAAAGTAAAGTCGGAAATTCGTTTCTCAATATCTCTGACAATCTTATCGCGTCCCCTGGCCAGAAAAGTTCCAGAGCTTGTACGCACTCTGCTATCTTTGCTCTTTCCAGTCTCACTGTCGACAACAGTTGACTTGTGCATACTTGGCTTTGCTATGTCAATCAGATATTCACATTCCTCCTTGGTCAGAAAATTGTGATAGACAAAAGCTCTAGGCTCCCATGATACAACTTCAACCCAGCGCTCTCCCTTACCATCATCATCATCAACACTATCGACGGTGTTATGAGCAATGGAGGTGAGATCGTTGGGTTTAGGCAAGTTTTGATTGGAAGAAGGAATGGAAAGGATTCCGAAAACGAGGAGAATGAGGATAAGGAAAGTGAAAGCGATGAAGAGAGCGAAGATGATAGTGTAAGAAGAAGATGATTTTCTAGATGGAAGACGAGAGTATCTGGATTTGGCCATTGTTGAGTTTTCAGATCTACGAGATCTGAAATTCAACGAATGTAAAAACAAGTTGGAATTGAATAAAAGAGAGAACAAGAGATAATGCTGTTACGACTTGTTAGATGGTGAGAGTAGAACGAAGGAAAGAAAGAAAGAAGGAACNAGGAAATGATTTTTTATATTTTCTTTTATTTTCTATT
TARGET=972,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0236
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=ACATTCCTCCTTGGTCAGAAAA
PRIMER_RIGHT_SEQUENCE=TCACCTCCATTGCTCATAACAC
PRIMER_LEFT=888,22
PRIMER_RIGHT=1018,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=131
PRIMER_PAIR_PENALTY_1=0.0259
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=ACATTCCTCCTTGGTCAGAAAA
PRIMER_RIGHT_1_SEQUENCE=CTAAACCCAACGATCTCACCTC
PRIMER_LEFT_1=888,22
PRIMER_RIGHT_1=1033,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.0459
PRIMER_LEFT_2_PENALTY=0.045851
PRIMER_RIGHT_2_PENALTY=0.000037
PRIMER_LEFT_2_SEQUENCE=AAAAGTTCCAGAGCTTGTACGC
PRIMER_RIGHT_2_SEQUENCE=TCACCTCCATTGCTCATAACAC
PRIMER_LEFT_2=780,22
PRIMER_RIGHT_2=1018,22
PRIMER_LEFT_2_TM=59.954
PRIMER_RIGHT_2_TM=60.000
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.0482
PRIMER_LEFT_3_PENALTY=0.045851
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=AAAAGTTCCAGAGCTTGTACGC
PRIMER_RIGHT_3_SEQUENCE=CTAAACCCAACGATCTCACCTC
PRIMER_LEFT_3=780,22
PRIMER_RIGHT_3=1033,22
PRIMER_LEFT_3_TM=59.954
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.9000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.1262
PRIMER_LEFT_4_PENALTY=0.023556
PRIMER_RIGHT_4_PENALTY=0.102638
PRIMER_LEFT_4_SEQUENCE=ACATTCCTCCTTGGTCAGAAAA
PRIMER_RIGHT_4_SEQUENCE=GCCAAATCCAGATACTCTCGTC
PRIMER_LEFT_4=888,22
PRIMER_RIGHT_4=1181,22
PRIMER_LEFT_4_TM=59.976
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_04672 Ps_contig_mira-and-tgicl-ass_franssen_34220
SEQUENCE=ATTATGNGCTCGGAGGAAAAAGCTGGTATACTTGTTACATCTATTGGTTTCATACATTTTCTAGAATCTCTTATGCGAAGTTCACAAGTTCACAACTTCACAACTTCACAAGTACTTCCTTTTTTATTGTATTTGCTACATATAAATGAAAAAGGTAGGGAAAAAATGAGTTACCTAAGCTACATAACATAACATAAATTACAAAACGCCGCATCACACTATAACCATTTCCTTCAAGACCTGAAGCTCGCTGAGACTTTAAATATTGCACACGGTTGGAATGATGAACTCAGATAAGCCAATAATAATGTATACTCAAGTTTTGTATTCACTGACGCGGATCCATTTGGTACTTGACCACTTATTCCCTTTGATCACTGGACAACCACCATGCAAGCTTGACGCATCTAAAGTTGCATCCGGCTTCATGCTCCAAAAAAGCAAAGCATCGCCTCTCTTTGGCTTAATTGAAAGTCCTTTTTTCCCACAATCAGAAAGCTCGTTATACCATGGCACAGAACTAAAATTGCCCTTGGCAGCAGGGAACACTGTCTCGCCCCCTTCTTCAACATCTGTAAGGTACATCAATACTGTTGCTATGCGCTGACCACCATTTTCTGTGTTAAACTCATCAAGAAAGTAGTCATAGTGAGGCTCATACTTTTGTCCAACTTCATAATGTAGAACTTGAAGTCCTTCACCGTGCTCTACAGGAATAAAAGTAAAGTCGGAAATTCGTTTCTCAATATCTCTGACAATCTTATCGCGTCCCCTGGCCAGAAAAGTTCCAGAGCTTGTACGCACTCTGCTATCTTTGCTCTTTCCAGTCTCACTGTCGACAACAGTTGACTTGTGCATACTTGGCTTTGCTATGTCAATCAGATATTCACATTCCTCCTTGGTCAGAAAATTGTGATAGACAAAAGCTCTAGGCTCCCATGATACAACTTCAACCCAGCGCTCTCCCTTACCATCATCATCATCAACACTATCGACGGTGTTATGAGCAATGGAGGTGAGATCGTTGGGTTTAGGCAAGTTTTGATTGGAAGAAGGAATGGAAAGGATTCCGAAAACGAGGAGAATGAGGATAAGGAAAGTGAAAGCGATGAAGAGAGCGAAGATGATAGTGTAAGAAGAAGATGATTTTCTAGATGGAAGACGAGAGTATCTGGATTTGGCCATTGTTGAGTTTTCAGATCTACGAGATCTGAAATTCAACGAATGTAAAAACAAGTTGGAATTGAATAAAAGAGAGAACAAGAGATAATGCTGTTACGACTTGTTAGATGGTGAGAGTAGAACGAAGGAAAGAAAGAAAGAAGGAACNAGGAAATGATTTTTTATATTTTCTTTTATTTTCTATT
TARGET=1310,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04673 Ps_contig_mira-and-tgicl-ass_franssen_34221
SEQUENCE=AAAGAACTGCTTTGTAGCAACTNGTTACAGCTGTGTCCATGGTCTCTCTCCTTTGAAATCCTTTTTGAGTTAAGCCACAACTGAAACAAGCAGATCGAACTCCCAATTCACCACTTGCTGTTGTTGACTCTAGTCAAGATCCGATTTTGATCCCAAAACACCACCACCACCACCATGAGCGTCATCGACCTTCTAACCCGAGTTGATTCCATTTGTAAAAAGTACGACAAATACGACCTCGACAAGAACAAGGATGCCAATGTCACCGGCGACGATGCTTTCGTCAGACTCTACGCCTCCGTCGACTCCGACATTGAAAGCCTACTTCAGAAAGCAGAAACCGCTTCCAAGGAGAGAGGGAAGGCATCTGCTGTAGCGATCAATGCGGAGATTCGTCGTACCAAGTCTCGGTTGTTGGAGGAAGTTCCCAAGTTGCAGAGGTTGGCTGTCAAAAAGGTAAAAGGGCTTTCGTCGCAAGAGTTTGCCGCCCGTAATGATCTGGTTCTTGCATTACCGGAAAGAATCCAGGCTATCCCAGATGGGACTCCTGTTGCGCCAAAACAAACTGGTGGCTGGGCAGCTTCTTCTGCCTCACGCGCTGAAATTAAATTTGATTCGGATGGCCGATTTGATGACGAATACTTCAAATCAACTGAGCAATCTAGTCAATTCAGACAGGAATATGAAATGCGTAGAATAAAACAGGATCAAGGTTTGGATATGATTGCAGAAGGGTTGGATACTTTGAAAGACATGGCACATGATATGAATGAGGAACTGGATAGACAAGTTCCCTTGATGGATGAGATTGATACCAAGGTGGACAGGGCATCATCTGATCTTAAGAATACTAATGTTAGACTTAAAGACACGGTGAATCAGCTTCGATCCAGTCGAAACTTCTGTATTGATATTGTTTTGTTGATTATAATTCTGGGAATTGCCGCCTACTTATACAATGTGCTTAAATGATATGATACGTGAAGACTATCAATGTATTTCTACAATGCCTGTATTAATTTGAGAATATTGTGTCTGATTTTATTTGATTGTTAAATTTTTATCATTGTGAAAATTTCCGCCCCTCTTGAGAGATAGCCTGTCCCAATACCCTTTACAAAGCAGCCTGTGAATGTTGTGTTACACACAATTAATATGTTAAGTAGCTGCGGCTTGTTTTATGTACTTGTTAAGTGTTATACTTGAATTGATATTCTTGTGTTTTATATAACATTATGCTTGTTTTATTATAGGATACCGAGAGATTAGTCTGAAAACCTT
TARGET=159,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2115
PRIMER_LEFT_PENALTY=0.073989
PRIMER_RIGHT_PENALTY=0.137489
PRIMER_LEFT_SEQUENCE=CAATTCACCACTTGCTGTTGTT
PRIMER_RIGHT_SEQUENCE=GTAGGCTTTCAATGTCGGAGTC
PRIMER_LEFT=103,22
PRIMER_RIGHT=324,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=60.137
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.2115
PRIMER_LEFT_1_PENALTY=0.073989
PRIMER_RIGHT_1_PENALTY=0.137489
PRIMER_LEFT_1_SEQUENCE=AATTCACCACTTGCTGTTGTTG
PRIMER_RIGHT_1_SEQUENCE=GTAGGCTTTCAATGTCGGAGTC
PRIMER_LEFT_1=104,22
PRIMER_RIGHT_1=324,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.137
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.2906
PRIMER_LEFT_2_PENALTY=0.073989
PRIMER_RIGHT_2_PENALTY=0.216588
PRIMER_LEFT_2_SEQUENCE=CAATTCACCACTTGCTGTTGTT
PRIMER_RIGHT_2_SEQUENCE=AAGTAGGCTTTCAATGTCGGAG
PRIMER_LEFT_2=103,22
PRIMER_RIGHT_2=326,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.783
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.2906
PRIMER_LEFT_3_PENALTY=0.073989
PRIMER_RIGHT_3_PENALTY=0.216588
PRIMER_LEFT_3_SEQUENCE=AATTCACCACTTGCTGTTGTTG
PRIMER_RIGHT_3_SEQUENCE=AAGTAGGCTTTCAATGTCGGAG
PRIMER_LEFT_3=104,22
PRIMER_RIGHT_3=326,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.783
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.5330
PRIMER_LEFT_4_PENALTY=0.395499
PRIMER_RIGHT_4_PENALTY=0.137489
PRIMER_LEFT_4_SEQUENCE=GTGTCCATGGTCTCTCTCCTTT
PRIMER_RIGHT_4_SEQUENCE=GTAGGCTTTCAATGTCGGAGTC
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=324,22
PRIMER_LEFT_4_TM=59.605
PRIMER_RIGHT_4_TM=60.137
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_04674 Ps_contig_mira-and-tgicl-ass_franssen_34222
SEQUENCE=CCTTTTAGTCTAGAAATTAAAAACAATACAACACAAGTTTGAATTTAAAACTAAGTAACAGAAGAAAAAAAAAAGTACCTTCACATTAACAAAACTAGATTGAATACCCATGAATTTCTATTTCAGATTATGAATGACTTTGGCTCATTCTGCCATCTTATCTTCTTCCTCCTTCTCATCATCATCATCATCATCATCATCTCTCAGTTCAAGATCATCAAGCTCAGCCAGCAACTCATTGAATGGAACAGAAGGCAGCTCTTCACCATCAGCAACAGATGTCGCCTCAGATGGCTGATAATCTTTATTTTGGTATAAAGATATGTTGAACCGCAACTCAGGGTTTTCTTCAATGTCTTTCAAGAATTGTTCATATTCTGAGGCCATCTTATCTTCATTAACCTTCTCATCAACTTCCATGACAAGTGACTTAAGCTTCCATGCACGAGGCTTCGGGCGTCGCTTCTGGCGTTTCTCTTCATAACTCTTCTTTATCAAAACCACTTCAGGAACATAACCTCTGTAATTGTCCAATTCCATGTCATTAGTGTTAGCACTATACAAGTCATAACCAAGTGCCAAGTCACCAGGCTTTAAAAGATGGCCCAAGTGAGTTTTGATATTGAACATTATGTCATTCTTTCCGAAATCCGACACACGAGCCACTTGAGCATCAGCTAAAACATATTTCGTGCCACCAACGATGACCTCAGATGAAACAGGCTCAATATCCAACACGATATATTCGACTAACTGTCTGCTAGTAAGTAAGGACTTAAAGGATGTCCTCCAATACTGATCAGCGTCTAAGAAACCGAATTTCAGGGTATGTGGATCAAGCAAAGCAATGCTGTTAGTAACCTTAGTGCAAATCACAATAGGACCGATATTTCCCAAGGTTCCAGCCGCTTTAGGAGGCAAACAAATCAAATCCTCGCGACAAATGGGACTGATTTCAACAGAGAATGTGTACTTATAATTATAAATACTACTCTTTACATCATGAGAAACAAGCTGCTTATCACTACGGCTCCTTATCGGAGATACTTTCCCTATAAATTCAACAAACTTGTTACCATGACTTCGACTGGTGAAATAGAAATCAATACCATGATCCATCTGTTTGATTCGGACAGCACGAGCTGCAGCACCGTGCTTCAGAATCAGCTGCTCCAAATAAAAGAAAGTCCGCCGATGAGAGACATGCTGCCTGAGTTGCACAGCAGCAACCCACTGATCAGGATTCGCCGCAACCTTCGAACAAGATTCACACATGTGATCCTGTTGAACATACTCGACAAGATAAGACTGTTCCAAAATCGCGCCATGATAAACCTCCTTCTGAACACTGACCTTAACTTTAACCCTCTTGGAATGTGGCTCAGTCCAAATAAACTCAGCATTTACCAACTTAGCTTTGTTGATATTCATATTCTTCTGCAACCTTTTCAAGCAAAATGTCAAGAGCTCCTTCGATTCTAATTGAAGTTTGACCCATGTTCTTGGTGGCTGCAAGTAACTCTCGCAATCAGGGCAATGAACAAGCACGAGACGCTTGAGTAACCCTTCGGTGATATCAACTTCGGAGCGCAAACACTTAACACACATATTGGCTGCATTTGGTTGCATTGGAATCCCACATTTGCAACACAAAACACTGCCAATTGTTCGTTGAACAGTGAACATGCCAGTTTCTCCAGCCATCTCTAATAAACCCTGAAGAATCAAAGTGGAAGCAGGAGGTATGGTGAAGAAGAAATGAAAAACCACGATACGAAGGAGATGAATCAGCGGCGTGTTTGATTTACGGTGGCTGCCGAAAATTTTGCTAGGTTTACAATAAATTGTTAATATCATACCCCGAC
TARGET=176,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3667
PRIMER_LEFT_PENALTY=0.248900
PRIMER_RIGHT_PENALTY=0.117817
PRIMER_LEFT_SEQUENCE=TGAATGACTTTGGCTCATTCTG
PRIMER_RIGHT_SEQUENCE=TCTGTTCCATTCAATGAGTTGC
PRIMER_LEFT=130,22
PRIMER_RIGHT=251,22
PRIMER_LEFT_TM=60.249
PRIMER_RIGHT_TM=60.118
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=122
PRIMER_PAIR_PENALTY_1=0.4030
PRIMER_LEFT_1_PENALTY=0.285163
PRIMER_RIGHT_1_PENALTY=0.117817
PRIMER_LEFT_1_SEQUENCE=GGCTCATTCTGCCATCTTATCT
PRIMER_RIGHT_1_SEQUENCE=TCTGTTCCATTCAATGAGTTGC
PRIMER_LEFT_1=141,22
PRIMER_RIGHT_1=251,22
PRIMER_LEFT_1_TM=59.715
PRIMER_RIGHT_1_TM=60.118
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=0.4276
PRIMER_LEFT_2_PENALTY=0.248900
PRIMER_RIGHT_2_PENALTY=0.178718
PRIMER_LEFT_2_SEQUENCE=TGAATGACTTTGGCTCATTCTG
PRIMER_RIGHT_2_SEQUENCE=ACATCTGTTGCTGATGGTGAAG
PRIMER_LEFT_2=130,22
PRIMER_RIGHT_2=281,22
PRIMER_LEFT_2_TM=60.249
PRIMER_RIGHT_2_TM=60.179
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.4301
PRIMER_LEFT_3_PENALTY=0.312329
PRIMER_RIGHT_3_PENALTY=0.117817
PRIMER_LEFT_3_SEQUENCE=TGCCATCTTATCTTCTTCCTCC
PRIMER_RIGHT_3_SEQUENCE=TCTGTTCCATTCAATGAGTTGC
PRIMER_LEFT_3=150,22
PRIMER_RIGHT_3=251,22
PRIMER_LEFT_3_TM=59.688
PRIMER_RIGHT_3_TM=60.118
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=102
PRIMER_PAIR_PENALTY_4=0.4639
PRIMER_LEFT_4_PENALTY=0.285163
PRIMER_RIGHT_4_PENALTY=0.178718
PRIMER_LEFT_4_SEQUENCE=GGCTCATTCTGCCATCTTATCT
PRIMER_RIGHT_4_SEQUENCE=ACATCTGTTGCTGATGGTGAAG
PRIMER_LEFT_4=141,22
PRIMER_RIGHT_4=281,22
PRIMER_LEFT_4_TM=59.715
PRIMER_RIGHT_4_TM=60.179
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_04675 Ps_contig_mira-and-tgicl-ass_franssen_34229
SEQUENCE=TACCTATTCGTCTCCGAGACGCTGGCGATCAAAACATTGATCTTTGTCACATTTGCGGGACTAAGGATAAGAGACGTGGAGATTGTTTCTCGGTCGGTTTTGCCTAGGTTTTACGCCGAGGACGTGCATCCGGTGACGTGGAACGTGTTCAATTCGTTCGGGAAACGGTATGTTGTTACCGCGAGTCCGAGGATTATGGTTGAGCCTTTTGTTAAGACGTTTTTGGGAGTTGATAAGGTGCTTGGAACTGAATTGATGGTTACAAAAAGAGGGAGAGCAACAGGGTTTGTTAAGGAACCTGGAGTTCTTGTTGGTGAACACAAGAAAGAGGCAGTTATGAAAGAATTTGGAAGTAACAACTTGCCTGATTTGGGACTTGGAGACAGTGTAACTGACTCTGACTTCATGTCAATTTGCAAGGAAGGTTACATGGTGCCAAGGACCAAATGTGACGCATTACCAAGAAACAAGCTTCTAAGCCCAATAATTTTTCATGAGGGTAGATTAGTCCAAAAACCAACCCCTCTAGTTGCACTCTTAACCTTCCTATGGATGCCAATTGGGATCATACTTTCAATCTTAAGGGTCTATCTCAACATACCTTTGCCTGAAAGAATTGCTTGGTACAATTATAAGTTACTTGGAATAAGAGTAATTAGAAAAGGTACACCTCCACCACCACCAAAAAATGGACAAAGTGGTGTACTATTTATTTGCAACCATAGAACAGTTTTGGATCCGGTTGTTACAGCTGTTGCATTAGGAAGAAAAATAAGTTGTGTAACTTATAGTATAAGTAAGTTCACCGAGATTATTTCGCCTATAAAAGCAGTCGCACTTACTAGGGAAAGAGACAGAGACGCTGCAAATATAAAGAAATTGCTTGAAGAAGGCGATTTAGTAATTTGTCCTGAAGGAACGACTTGTAGGGAGCCGTTTCTATTGAGGTTTAGTGCTCTTTTTGCTGAGCTAACAGATAGGATTGTCCCGGTCGCGATCAATACGAAACAAAGTATGTTTTATGGAACTTCAGTTCGGGGACACAAATTGTTGGATCCTTATTTTGTGTTCATGAACCCTATGCCGACTTATGAGATCACTTTCTTGAACCAATTGCCTTTGGAGTTGACTTGCAATGGAGGGAAATCTTCAATTGAAGTTGCAAATTATATCCAAAGGGTTCTTGGAGGGACTCTTGGATTTGAATGTACCAATCTAACAAGGAAGGATAAGTATGCTATGCTTGCAGGAACAGACGGAATCGTTGGATCAAAGAAGAAAGATTAAAATCGAGAAGAACAATTCAACCGATTTTGATTTTTTATAAACTATTAAAAAAATGGAATTTTAAATAAAAAGTTACAGACAAAAGAAAAAAATGTGTCTCTAATTTCGTCACTAAAAATAAGAATGACGGAATCGAAGACTAATTTTATTTTGTCTGTCTATTTTTTTTTTGAAAATTCGTTTTTTTAAATAGTGATTATTGTCATTTTCCATGATGTTTAATTTTAAAGTTCTTACAATTATCAGAATAATAAAAAAAATAATTGTGTTTATTATGCTCCTAAAAATAACATTAATATGTACATTATTATTATTTATTGTTATTATTATTCTGATGAATACAAATGGTTTATTTATGTCAATTGTAAAATCTAGTACATCTTTGTGATGYTAAAGTTCTACTATTTATACATATA
TARGET=673,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04676 Ps_contig_mira-and-tgicl-ass_franssen_34229
SEQUENCE=TACCTATTCGTCTCCGAGACGCTGGCGATCAAAACATTGATCTTTGTCACATTTGCGGGACTAAGGATAAGAGACGTGGAGATTGTTTCTCGGTCGGTTTTGCCTAGGTTTTACGCCGAGGACGTGCATCCGGTGACGTGGAACGTGTTCAATTCGTTCGGGAAACGGTATGTTGTTACCGCGAGTCCGAGGATTATGGTTGAGCCTTTTGTTAAGACGTTTTTGGGAGTTGATAAGGTGCTTGGAACTGAATTGATGGTTACAAAAAGAGGGAGAGCAACAGGGTTTGTTAAGGAACCTGGAGTTCTTGTTGGTGAACACAAGAAAGAGGCAGTTATGAAAGAATTTGGAAGTAACAACTTGCCTGATTTGGGACTTGGAGACAGTGTAACTGACTCTGACTTCATGTCAATTTGCAAGGAAGGTTACATGGTGCCAAGGACCAAATGTGACGCATTACCAAGAAACAAGCTTCTAAGCCCAATAATTTTTCATGAGGGTAGATTAGTCCAAAAACCAACCCCTCTAGTTGCACTCTTAACCTTCCTATGGATGCCAATTGGGATCATACTTTCAATCTTAAGGGTCTATCTCAACATACCTTTGCCTGAAAGAATTGCTTGGTACAATTATAAGTTACTTGGAATAAGAGTAATTAGAAAAGGTACACCTCCACCACCACCAAAAAATGGACAAAGTGGTGTACTATTTATTTGCAACCATAGAACAGTTTTGGATCCGGTTGTTACAGCTGTTGCATTAGGAAGAAAAATAAGTTGTGTAACTTATAGTATAAGTAAGTTCACCGAGATTATTTCGCCTATAAAAGCAGTCGCACTTACTAGGGAAAGAGACAGAGACGCTGCAAATATAAAGAAATTGCTTGAAGAAGGCGATTTAGTAATTTGTCCTGAAGGAACGACTTGTAGGGAGCCGTTTCTATTGAGGTTTAGTGCTCTTTTTGCTGAGCTAACAGATAGGATTGTCCCGGTCGCGATCAATACGAAACAAAGTATGTTTTATGGAACTTCAGTTCGGGGACACAAATTGTTGGATCCTTATTTTGTGTTCATGAACCCTATGCCGACTTATGAGATCACTTTCTTGAACCAATTGCCTTTGGAGTTGACTTGCAATGGAGGGAAATCTTCAATTGAAGTTGCAAATTATATCCAAAGGGTTCTTGGAGGGACTCTTGGATTTGAATGTACCAATCTAACAAGGAAGGATAAGTATGCTATGCTTGCAGGAACAGACGGAATCGTTGGATCAAAGAAGAAAGATTAAAATCGAGAAGAACAATTCAACCGATTTTGATTTTTTATAAACTATTAAAAAAATGGAATTTTAAATAAAAAGTTACAGACAAAAGAAAAAAATGTGTCTCTAATTTCGTCACTAAAAATAAGAATGACGGAATCGAAGACTAATTTTATTTTGTCTGTCTATTTTTTTTTTGAAAATTCGTTTTTTTAAATAGTGATTATTGTCATTTTCCATGATGTTTAATTTTAAAGTTCTTACAATTATCAGAATAATAAAAAAAATAATTGTGTTTATTATGCTCCTAAAAATAACATTAATATGTACATTATTATTATTTATTGTTATTATTATTCTGATGAATACAAATGGTTTATTTATGTCAATTGTAAAATCTAGTACATCTTTGTGATGYTAAAGTTCTACTATTTATACATATA
TARGET=1591,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04677 Ps_contig_mira-and-tgicl-ass_franssen_34237
SEQUENCE=ACTTAATGAACAAAAAAAACTAATTAATNACNNYATAATCCTACAAAATATCATTACAAATAAANNAAAAAAATAAACTAATGCATATTACTATTAATAAAAATCTGAARAGTTTCGTTAGCGAAAGTTAATCAAAGAGATCACAAATAAAATAATATCCCTTATTGTACGGTCAAGTCAATCGTGCGGCTGAGAACTCTCCTGGCCTTTTCCGTCACTTACTTGCCGGATCGACGCCTTCGACGACGGTTCTCCATCCCACCTCCTCCTCTCAGCACCGTACCTACGTTGATTTACGCAAATTTTCGAATCTGAAGCCTGAAAATCACGATCCTCCTCACGACGTCGTTTCAGAGAAACTAAGTTTGTTGTAACTAACTTCGAAACAACCTCCTCATCGTTATCGTTGCACTTACTAACCGACTCTTCGCTACCGGAAGAACTCGAAGCCGCCGGCGGCTCCGCCGTAGAAGACCAAGGAACCACCTGATTATCACCATCCTCATCACCGTCAACCTCCTCTTCCTCTTCCTCTTCCTCATCAAAATCAGTATCAACCTCATCGTCCTCCTCATTGTCACCTTCACTCTCTTCCTCCTGCTCACCTTCCAATTTCCTTCCACCACCACCGCGGCCGCACGTTTCGCACAGAGAAAGCGCGTTACTAAGCCTAGCACCGGAGGCTTTCCACGGCGTCGGAGACTGGCACCGGTGACAGAGGAGAGTCCTCACGTGTCTGGCAACAAGGAAATTTGCACTGTGGATCTTAGAATCGCAGTCCCAGCATAAGCTAGCCTGGTCGGACTCACAGAAAGTTCTAGCCGGAAGTTTGCAGAGTTCGCAGTTTTTCTTCTATTTTTCTTTGATCAGAGTTCTTTCTTTAGTTTTTTATTTTTTTTGCTATCAACGTCGGTTTCNCTTATTTCTTTCTTTCTGTGTGTTATTTTTCGTTTTTTNTTCTTCTTCTTNTTGTGTGTTGGTTTGGTTGGATGAAGAGTAGGGAAGAAGGAAGAAAG
TARGET=923,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04678 Ps_contig_mira-and-tgicl-ass_franssen_34238
SEQUENCE=GAACTTAACAGAGAGAAARTNRRNRRNRAANTCCAAAAAAANACAATGGAAGGAGGAGGAATGTTATCATCATCATCATCACCACATTCACAAAACTATATCTACAGAGAGTGTTTAAGAAACCATGCAGCCAGCCTCGGAAGCTACGCAACCGACGGCTGCGGAGAATTCACCGTCGACGACACTTCAGTATCAGCAGCCAACAGTCTCCAATGTGCAGCCTGTGGCTGTCACCGGAACTTCCATCGCAAGATAACCGCGACATATGCGACGATGGCGCGAGATAACGCCATTGTGGCAGTGTCGGATCAGGTGATGGAATATTCCGGTGGTGGAGATGGGAGGAGGAAGAGGTACAGGTCAAAATTCACGGCGGATCAGAAGGAGAAGATGCTGGGGTTTGCTGAGAAGCTAGGGTGGAAGTTGCAGAGGAAGGAACTTGATGAAGAAATTGAAAGGTTTTGTGAGAGTGTTGGTGTGAGTAGGCAGGTTTTTAAGGTTTGGATGCATAATCATAAGAATTCTTGTTTCTCTAATTCTTCTGATCCTTCTGCTGGTAATGCTAATTCTTCTCTTACTCAGTAGTTGTGTTGTGTGTGAAGGAATTTGGAAAATTAATGTAGTATTTTAATTATTGCTTTATTTTTGTTTTTTTCTTTTTAATTGTTTTTTTTTTCTATCATGACTTGTGTGTAATAATTTTTTATGATTTATTTTTGTTTTGCTTTCAGGAAATTAA
TARGET=66,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04679 Ps_contig_mira-and-tgicl-ass_franssen_34249
SEQUENCE=ATTATTATTATTATTRRNNNATTATCATCATTGTTGTTGTTAACGRRNGAATGAATCCGCCACACAGCACACAATACAGCACAACAACATCATCCCTCACGCCAAATAAACGATTCCTCTCCTCATCCTCATCCTCCCATCTCTCTCCACAACCAACATCACCTCGTTTCGGGCCAAACTCGACCCGGATCACAACCCGTTTCCTTCAACCACTTCCGCCTTGTTCCAAACAACCAGTATCAAATACTGCAAAAGCTAAATCGACTCTTTCACTAGATAATCACACCACCATTAAAACGATCGTTCATTTCTACTCGTGTATTCGTTTTTGTAGCTGGTCAAAATGGTGGGTTTATTCTCGCGATTCACCGTTAAAAAGAAGCATGCATCGACGCACGCAAAGTGCCCGCTTGGAGGAGAGGGATGTACTTCCTGCAAACGCAGAGGCGGCGGCGGCTTCTGTTGCTGCAAGTGCGGCGGCCACTGCAACTTCTCATGGAATTGAAGTGGCTGTGGAATTTAAGCCAGTTGAACATCCGGTTGAGCCTCTTGACAATGATCGACCAGTTCAATGTCCGTTGCCAGAACCATCTATTCTTAATGATGGAAGAATATGGAAAGAGCGAGCATCTGCTACCATGCATAGAAGAGGTGACCTGCCAGTAATGAAAGAAGGGGAATCCCTCGAGCATGAAGGTGGTGGTAATACTCGGCCGCGAAGATCACGTTCTAATCGAATGATCCTACCATCTGTTAGTGCTCCTGAGCATAATCTTCTAAAACTTCTTGAAGAGTCGGGCATCTAAACTGGTATTGTTTTTATGGGTGTATTTTGGTGGCATAAATTTTATCAACTTAAATGTGGAATACAATTTTTTGTCATCCTTTACTAGCTCAATGTTATTTATACCCCCGAATATGCGTATATTCATATGTATAATAATAATAGTAAACAAAAGATTTTTACTTGCTGTTTGATATGATATAATTTGAATTTATACTTTTGGTACCAAAAT
TARGET=1,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04680 Ps_contig_mira-and-tgicl-ass_franssen_34249
SEQUENCE=ATTATTATTATTATTRRNNNATTATCATCATTGTTGTTGTTAACGRRNGAATGAATCCGCCACACAGCACACAATACAGCACAACAACATCATCCCTCACGCCAAATAAACGATTCCTCTCCTCATCCTCATCCTCCCATCTCTCTCCACAACCAACATCACCTCGTTTCGGGCCAAACTCGACCCGGATCACAACCCGTTTCCTTCAACCACTTCCGCCTTGTTCCAAACAACCAGTATCAAATACTGCAAAAGCTAAATCGACTCTTTCACTAGATAATCACACCACCATTAAAACGATCGTTCATTTCTACTCGTGTATTCGTTTTTGTAGCTGGTCAAAATGGTGGGTTTATTCTCGCGATTCACCGTTAAAAAGAAGCATGCATCGACGCACGCAAAGTGCCCGCTTGGAGGAGAGGGATGTACTTCCTGCAAACGCAGAGGCGGCGGCGGCTTCTGTTGCTGCAAGTGCGGCGGCCACTGCAACTTCTCATGGAATTGAAGTGGCTGTGGAATTTAAGCCAGTTGAACATCCGGTTGAGCCTCTTGACAATGATCGACCAGTTCAATGTCCGTTGCCAGAACCATCTATTCTTAATGATGGAAGAATATGGAAAGAGCGAGCATCTGCTACCATGCATAGAAGAGGTGACCTGCCAGTAATGAAAGAAGGGGAATCCCTCGAGCATGAAGGTGGTGGTAATACTCGGCCGCGAAGATCACGTTCTAATCGAATGATCCTACCATCTGTTAGTGCTCCTGAGCATAATCTTCTAAAACTTCTTGAAGAGTCGGGCATCTAAACTGGTATTGTTTTTATGGGTGTATTTTGGTGGCATAAATTTTATCAACTTAAATGTGGAATACAATTTTTTGTCATCCTTTACTAGCTCAATGTTATTTATACCCCCGAATATGCGTATATTCATATGTATAATAATAATAGTAAACAAAAGATTTTTACTTGCTGTTTGATATGATATAATTTGAATTTATACTTTTGGTACCAAAAT
TARGET=446,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04681 Ps_contig_mira-and-tgicl-ass_franssen_34249
SEQUENCE=ATTATTATTATTATTRRNNNATTATCATCATTGTTGTTGTTAACGRRNGAATGAATCCGCCACACAGCACACAATACAGCACAACAACATCATCCCTCACGCCAAATAAACGATTCCTCTCCTCATCCTCATCCTCCCATCTCTCTCCACAACCAACATCACCTCGTTTCGGGCCAAACTCGACCCGGATCACAACCCGTTTCCTTCAACCACTTCCGCCTTGTTCCAAACAACCAGTATCAAATACTGCAAAAGCTAAATCGACTCTTTCACTAGATAATCACACCACCATTAAAACGATCGTTCATTTCTACTCGTGTATTCGTTTTTGTAGCTGGTCAAAATGGTGGGTTTATTCTCGCGATTCACCGTTAAAAAGAAGCATGCATCGACGCACGCAAAGTGCCCGCTTGGAGGAGAGGGATGTACTTCCTGCAAACGCAGAGGCGGCGGCGGCTTCTGTTGCTGCAAGTGCGGCGGCCACTGCAACTTCTCATGGAATTGAAGTGGCTGTGGAATTTAAGCCAGTTGAACATCCGGTTGAGCCTCTTGACAATGATCGACCAGTTCAATGTCCGTTGCCAGAACCATCTATTCTTAATGATGGAAGAATATGGAAAGAGCGAGCATCTGCTACCATGCATAGAAGAGGTGACCTGCCAGTAATGAAAGAAGGGGAATCCCTCGAGCATGAAGGTGGTGGTAATACTCGGCCGCGAAGATCACGTTCTAATCGAATGATCCTACCATCTGTTAGTGCTCCTGAGCATAATCTTCTAAAACTTCTTGAAGAGTCGGGCATCTAAACTGGTATTGTTTTTATGGGTGTATTTTGGTGGCATAAATTTTATCAACTTAAATGTGGAATACAATTTTTTGTCATCCTTTACTAGCTCAATGTTATTTATACCCCCGAATATGCGTATATTCATATGTATAATAATAATAGTAAACAAAAGATTTTTACTTGCTGTTTGATATGATATAATTTGAATTTATACTTTTGGTACCAAAAT
TARGET=937,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04682 Ps_contig_mira-and-tgicl-ass_franssen_34251
SEQUENCE=CTAATATGCAAGAAACTGAAGAAGACTCATTCAATTAAAGTTGTTATTTTGACAACCGTTACATATAATTGCTTTATATAATATGCAAGAAAAGTAAATAACTCATGAAAATAAAAAACTACGCAACTACATTAAAAAAGTAGCACTCATAATTACTATGATGGCACTTGCTTTATTAAGAGCAAGTTGCAAAAACCATTGTTTTTCAAAGTGAAGTGATTGCTCATTCTTCATCCCATAGATCATCAAGAAGAGGCTTGGTTGGTCCTTTGATGAGTCTCTCTTGATGAATTTCACTTATTTTCTTGTAATCATCCTCACTCAATGACCAGTCAAATATTTGCAAGTTTTGGTTCATCCTCTCCTTGTCATAGCTCTTTACCACAAATGTCAATCCTTGTTCATATAACCATCTAAGACAAATCTGAGCTATAGTCTTGCCATGAGCATCTGCCAATTCTTTGAGTATATCATTGTCCATCACTAAATTAGCTCCTCTGCTAGCACCCTTTCTCAGGGGTGAGAACGCAGTTACGATTATACCCTTTTCCTTGCAGAAAGCTCTAAGCTTCTCTTGTTGCCATCCAAGGTTAACTTCCACTTGATTCACTGCTGGAGGGATGGTTGCAAAGGATAGCAATTTCTGAAGCTTCTTGATAGAAAAGTTGCTAGCTCCAATAGCTTTGGTGAGACCAAGTTTTTGACATTCTTCCAAAGATGTCCACACACCTTTTAGGTCAAATTCCACAATATCTGATACTTCAATAGGGTATTTAACTTCACCGGGATTAGTAGTAATTGGCCAATGGATCAAAATTAGGTCTAAATATTCTAGTTGAAGAGTCCTGAGAGATTTCTTTAGAGCAGGAACGATGAGGTGAGGATGATTATCGGTAACCCACAATTTAGACGTAACAAAGAGTTCATCTCTAGATGAAATGAGTCCAAGTTTAAGTGCTTCAGCTATGGCTTCACCAACAGAATGTTCAACGCCATATGCAGCAGCAGCATCAAAATGTCTGTAACCTTGTTTGATTGCTTCAAGGACAGCATCTTTTGTAGTAACCTTACTGGTTGCTTCCGGTGCGGTTCCGAGGCCCATCACCGGCATTTTGCGTTGTCCGGTGGAGGAAGGGAGGACTATTTCAGGGACTGTAAGTGCAGCAGCCATTTTGTTTGTTAAGTGATGAGAAGAATGTTGAATTGGATGTAGCTATTGTAGTAGTTGTGTTGTCATCGACTA
TARGET=999,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1928
PRIMER_LEFT_PENALTY=0.154023
PRIMER_RIGHT_PENALTY=0.038744
PRIMER_LEFT_SEQUENCE=GCTTCACCAACAGAATGTTCAA
PRIMER_RIGHT_SEQUENCE=CCTGAAATAGTCCTCCCTTCCT
PRIMER_LEFT=968,22
PRIMER_RIGHT=1149,22
PRIMER_LEFT_TM=60.154
PRIMER_RIGHT_TM=59.961
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=0.3153
PRIMER_LEFT_1_PENALTY=0.154023
PRIMER_RIGHT_1_PENALTY=0.161311
PRIMER_LEFT_1_SEQUENCE=GCTTCACCAACAGAATGTTCAA
PRIMER_RIGHT_1_SEQUENCE=AAATAGTCCTCCCTTCCTCCAC
PRIMER_LEFT_1=968,22
PRIMER_RIGHT_1=1145,22
PRIMER_LEFT_1_TM=60.154
PRIMER_RIGHT_1_TM=59.839
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.3153
PRIMER_LEFT_2_PENALTY=0.154023
PRIMER_RIGHT_2_PENALTY=0.161311
PRIMER_LEFT_2_SEQUENCE=GCTTCACCAACAGAATGTTCAA
PRIMER_RIGHT_2_SEQUENCE=GTCCCTGAAATAGTCCTCCCTT
PRIMER_LEFT_2=968,22
PRIMER_RIGHT_2=1152,22
PRIMER_LEFT_2_TM=60.154
PRIMER_RIGHT_2_TM=59.839
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.3347
PRIMER_LEFT_3_PENALTY=0.173391
PRIMER_RIGHT_3_PENALTY=0.161311
PRIMER_LEFT_3_SEQUENCE=ATGAGGTGAGGATGATTATCGG
PRIMER_RIGHT_3_SEQUENCE=AAATAGTCCTCCCTTCCTCCAC
PRIMER_LEFT_3=872,22
PRIMER_RIGHT_3=1145,22
PRIMER_LEFT_3_TM=60.173
PRIMER_RIGHT_3_TM=59.839
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.8000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.4069
PRIMER_LEFT_4_PENALTY=0.368190
PRIMER_RIGHT_4_PENALTY=0.038744
PRIMER_LEFT_4_SEQUENCE=CTATGGCTTCACCAACAGAATG
PRIMER_RIGHT_4_SEQUENCE=CCTGAAATAGTCCTCCCTTCCT
PRIMER_LEFT_4=963,22
PRIMER_RIGHT_4=1149,22
PRIMER_LEFT_4_TM=59.632
PRIMER_RIGHT_4_TM=59.961
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_04683 Ps_contig_mira-and-tgicl-ass_franssen_34256
SEQUENCE=GTATCATCCACATCTAAAATCCACGCATCTTTATGACCAGGAACGGGTATTTGAGATTTTGCATAGAAAATAATTTCATCTACGATGGTGTTCATGTCAAGTTCGTATTGTCCGCCAAGGATGTAATTTTCAACATGTTTATAGCAACAAGGTGGCACCGTCTTCCGCGGTTGCACATTATTATTGGTCTCCACCGCCAACCTCCAGCTCAGACCATACCTCTCATCATCAACCTCGGAGGAAGAAGAAGAATCTAATTCAGATATCATCATCATCTTCTGGAGTTTCTGAGAAGAATGAGCCTTATGGTTCTGCTGCCTATGCATTTTCAACAATAGGTTGGTCAATAAGCCCAACAAAAACGTTAACCCTGCCACTATGGTTTGCACAATCTTTATCTTTAGCATCATCTTATAAGG
TARGET=241,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1669
PRIMER_LEFT_PENALTY=0.099438
PRIMER_RIGHT_PENALTY=0.067419
PRIMER_LEFT_SEQUENCE=CTCCAGCTCAGACCATACCTCT
PRIMER_RIGHT_SEQUENCE=TAGTGGCAGGGTTAACGTTTTT
PRIMER_LEFT=201,22
PRIMER_RIGHT=378,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.3361
PRIMER_LEFT_1_PENALTY=0.099438
PRIMER_RIGHT_1_PENALTY=0.236697
PRIMER_LEFT_1_SEQUENCE=CTCCAGCTCAGACCATACCTCT
PRIMER_RIGHT_1_SEQUENCE=GTTGGGCTTATTGACCAACCTA
PRIMER_LEFT_1=201,22
PRIMER_RIGHT_1=356,22
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=60.237
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.3418
PRIMER_LEFT_2_PENALTY=0.099438
PRIMER_RIGHT_2_PENALTY=0.242357
PRIMER_LEFT_2_SEQUENCE=CTCCAGCTCAGACCATACCTCT
PRIMER_RIGHT_2_SEQUENCE=GTTTTTGTTGGGCTTATTGACC
PRIMER_LEFT_2=201,22
PRIMER_RIGHT_2=362,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=59.758
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=0.3472
PRIMER_LEFT_3_PENALTY=0.099438
PRIMER_RIGHT_3_PENALTY=0.247716
PRIMER_LEFT_3_SEQUENCE=CTCCAGCTCAGACCATACCTCT
PRIMER_RIGHT_3_SEQUENCE=GCAGAACCATAAGGCTCATTCT
PRIMER_LEFT_3=201,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=59.752
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=0.3625
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.263023
PRIMER_LEFT_4_SEQUENCE=CTCCAGCTCAGACCATACCTCT
PRIMER_RIGHT_4_SEQUENCE=TGGGCTTATTGACCAACCTATT
PRIMER_LEFT_4=201,22
PRIMER_RIGHT_4=354,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=59.737
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_04684 Ps_contig_mira-and-tgicl-ass_franssen_34256
SEQUENCE=GTATCATCCACATCTAAAATCCACGCATCTTTATGACCAGGAACGGGTATTTGAGATTTTGCATAGAAAATAATTTCATCTACGATGGTGTTCATGTCAAGTTCGTATTGTCCGCCAAGGATGTAATTTTCAACATGTTTATAGCAACAAGGTGGCACCGTCTTCCGCGGTTGCACATTATTATTGGTCTCCACCGCCAACCTCCAGCTCAGACCATACCTCTCATCATCAACCTCGGAGGAAGAAGAAGAATCTAATTCAGATATCATCATCATCTTCTGGAGTTTCTGAGAAGAATGAGCCTTATGGTTCTGCTGCCTATGCATTTTCAACAATAGGTTGGTCAATAAGCCCAACAAAAACGTTAACCCTGCCACTATGGTTTGCACAATCTTTATCTTTAGCATCATCTTATAAGG
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1669
PRIMER_LEFT_PENALTY=0.099438
PRIMER_RIGHT_PENALTY=0.067419
PRIMER_LEFT_SEQUENCE=CTCCAGCTCAGACCATACCTCT
PRIMER_RIGHT_SEQUENCE=TAGTGGCAGGGTTAACGTTTTT
PRIMER_LEFT=201,22
PRIMER_RIGHT=378,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.3361
PRIMER_LEFT_1_PENALTY=0.099438
PRIMER_RIGHT_1_PENALTY=0.236697
PRIMER_LEFT_1_SEQUENCE=CTCCAGCTCAGACCATACCTCT
PRIMER_RIGHT_1_SEQUENCE=GTTGGGCTTATTGACCAACCTA
PRIMER_LEFT_1=201,22
PRIMER_RIGHT_1=356,22
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=60.237
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.3374
PRIMER_LEFT_2_PENALTY=0.269941
PRIMER_RIGHT_2_PENALTY=0.067419
PRIMER_LEFT_2_SEQUENCE=ACCTCGGAGGAAGAAGAAGAAT
PRIMER_RIGHT_2_SEQUENCE=TAGTGGCAGGGTTAACGTTTTT
PRIMER_LEFT_2=231,22
PRIMER_RIGHT_2=378,22
PRIMER_LEFT_2_TM=59.730
PRIMER_RIGHT_2_TM=59.933
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=0.3418
PRIMER_LEFT_3_PENALTY=0.099438
PRIMER_RIGHT_3_PENALTY=0.242357
PRIMER_LEFT_3_SEQUENCE=CTCCAGCTCAGACCATACCTCT
PRIMER_RIGHT_3_SEQUENCE=GTTTTTGTTGGGCTTATTGACC
PRIMER_LEFT_3=201,22
PRIMER_RIGHT_3=362,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=59.758
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=0.3472
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.247716
PRIMER_LEFT_4_SEQUENCE=CTCCAGCTCAGACCATACCTCT
PRIMER_RIGHT_4_SEQUENCE=GCAGAACCATAAGGCTCATTCT
PRIMER_LEFT_4=201,22
PRIMER_RIGHT_4=314,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=59.752
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=114
=
PRIMER_SEQUENCE_ID=SSR_04685 Ps_contig_mira-and-tgicl-ass_franssen_34257
SEQUENCE=ACTNTAATAGAGAGAGTGGATATTATTGAAAACATTACATAAAAGAAGATTATCTTACACGCACAAGGCACATAACACAAAGCTTATGGCACAAAAGTACCTAGCTATGATCACTTCTTTATTTCAACTAAAGTAACCCTCAAAAACACTTACATGGAACTAACCACACCCCCCTCAAATGATTCTGTAGAGGTCACGGCAGTAATATCCAACTCAGATGATTTCTTCTTTGTATTAACAAAATTGCATTGTTTTCTAATTTCACCTTTCTTTCCCGTAAGCACACCAATATTTCCCATTTTAATCATCGAAGTCGCAAAATTCTGAAAGAAAGCATTTTGGTTATTGGCAAAATTGTTGACAATGCCAATAGTATCTGCACCTGGTGTTGAGAACAATTCTTGATCACTTTGAAGCAAACCCTTTTTAACTTGAAGATTGTTGTAGTAGTTTTTGTCCAATATATCAGGAGTCGTTGGATCGAAATTGACACGGTTATCTCCGGCCCCGTTTTGAGGACACTGTTTTTGTAATACTTTTAAGTAAGTTGGATCAAGAGTTGGATCAGGTTTTCCAGTGTTGTTGAAGTTGAATAATCGGTCAGAGAATAAAAAGCAACGTGATCTTCCAAATGAATGTGCACCTGAGAGTGAAACTAGATCAAGTGTGGTGAGGCCTTGATCAGCAAAAGCAGATTTCAGTTCAGTTAAGCTGAAAGATGGACCTGGAAGGTTTTGATTAGCCAGAGTTTGATTTGCCGTTAAACTATCTCTTCTTCCTAATGGAACCAACCAACCTGTACCTCCGGTCAAAACGGAAGATATTCCAGCAGAAAGTGTAAGAATATCAGCACAAGAAACTTTGTTTGGACAAGCACTTTCTACAGCTGTTTTAATCTGATTTATAACATCTAAACCTCTTAAGGATTTGATATTTGGAAAGGCTTCTTGTTCAGTCACAATAGTAGAAGTTTTGTTCAACAAAACGGATGCATCACAACCTTGAACAAAGCAGTCATGAAAGTGGAGCCTGATGAGACTAGCAGGCATTCGGGGGTCGTTCTTAGCAACTTTGGATAAGACTTGAGATACTATAGCCTGCAACTGGGGGCAAGTTTGACTGTAAAAATTAGGGTCAAGTTGTGCATCTGAGGAGAAAGTTAATCCTCCAAACAGAACTGTTAAACAGCAGAGAGCTGTGGCTATGAGACGAAGGGAGTTCATATTTTCCCCACTGATATTAGAGCTATATGTGATTCCCCGCGTACTCTGCGTTGA
TARGET=786,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1190
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=CAAGAGTTGGATCAGGTTTTCC
PRIMER_RIGHT_SEQUENCE=TCTGCTGGAATATCTTCCGTTT
PRIMER_LEFT=553,22
PRIMER_RIGHT=832,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.1190
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=CAAGAGTTGGATCAGGTTTTCC
PRIMER_RIGHT_1_SEQUENCE=TTCTGCTGGAATATCTTCCGTT
PRIMER_LEFT_1=553,22
PRIMER_RIGHT_1=833,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.1406
PRIMER_LEFT_2_PENALTY=0.045112
PRIMER_RIGHT_2_PENALTY=0.095445
PRIMER_LEFT_2_SEQUENCE=CAGGTTTTCCAGTGTTGTTGAA
PRIMER_RIGHT_2_SEQUENCE=TCTGCTGGAATATCTTCCGTTT
PRIMER_LEFT_2=565,22
PRIMER_RIGHT_2=832,22
PRIMER_LEFT_2_TM=60.045
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.1406
PRIMER_LEFT_3_PENALTY=0.045112
PRIMER_RIGHT_3_PENALTY=0.095445
PRIMER_LEFT_3_SEQUENCE=CAGGTTTTCCAGTGTTGTTGAA
PRIMER_RIGHT_3_SEQUENCE=TTCTGCTGGAATATCTTCCGTT
PRIMER_LEFT_3=565,22
PRIMER_RIGHT_3=833,22
PRIMER_LEFT_3_TM=60.045
PRIMER_RIGHT_3_TM=60.095
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=0.2074
PRIMER_LEFT_4_PENALTY=0.023556
PRIMER_RIGHT_4_PENALTY=0.183834
PRIMER_LEFT_4_SEQUENCE=CAAGAGTTGGATCAGGTTTTCC
PRIMER_RIGHT_4_SEQUENCE=TGGAATATCTTCCGTTTTGACC
PRIMER_LEFT_4=553,22
PRIMER_RIGHT_4=827,22
PRIMER_LEFT_4_TM=59.976
PRIMER_RIGHT_4_TM=60.184
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_04686 Ps_contig_mira-and-tgicl-ass_franssen_34265
SEQUENCE=TTCGGGGGGTAGGAAGTGGCATCTCCATTTTCCATAAACTCATAAAAATAACACATAGTACGAAGTAATCGATGTATATGGATTACTTTACTACTACCACTCACAAGTTGTAACCCTCTCAACTAACTACCAGACAAATTCACCAATCTCAGCATCAACGTACCTAGATTCCCTTCTCTTCATGCTCTTTCTATTCTACTTATAACACAACTACCATTTTTTCCTCTTTCCTTTTTCCTACTATCTCACTTTCCATTTCTCAATCATGGCTTCTTTGTTTCACTGTCCCGCAACTCGATTAGGAGATTCTTCTGGCTTCTTTCTTCCCACCCGTAGTACCCGTTTCTTAAAGATTCATACCCGTAAGCCTCATCTTCGTTGCTCTTTGGATTCTAATGTTTCCGACATGAGTATCAATGCGCCAAAAGGATTGTTTCCTCCAGAGCCTGAACATTATCGAGGACCGAAGCTGAAAGTTGCTATCATTGGAGCTGGGCTTGCAGGCATGTCAACTGCCGTGGAACTTTTGGATCAAGGCCATGAGGTGGATATATATGAGTCGAGATCTTTTATTGGTGGAAAAGTTGGTTCTTTTGTTGATAAACGTGGAAATCATATTGAAATGGGATTGCATGTTTTCTTTGGTTGTTACAACAATCTTTTCCGACTAATGAAAAAGGTGGGTGCAGATAATAATCTACTTGTGAAGGATCACACTCACACTTTTGTAAACAAAGGGGGTCAAATTGGAGAACTAGATTTTCGGTTCCCGGTTGGGGCTCCATTACACGGGATAAGAGCATTTTTGACCACAAATCAGCTTAACACTTATGATAAGGCTAGAAATGCTGTGGCTCTTGCACTGAGTCCAGTTGTCCGAGCTCTTGTTGATCCAGATGGTGCATTGAGGGACATAAGGAATTTGGATAGTATTAGCTTTTCAGACTGGTTTATGTCTAAGGGTGGCACGCGCACGAGTATTCAAAAAATGTGGGATCCAGTTGCCTATGCCCTTGGGTTTATTGACTGTGATAATATCAGCGCCCGATGCATGCTCACCATATTTGCATTGTTTGCCACAAAGACCGAGGCTTCCCTTTTGCGAATGCTGAAGGGTTCACCAGATGTTTATTTGAGTGGACCTATCCAGAAGTACATCACAGATAGAGGGGGAAGGTTCCATCTTAGGTGGGGATGCAGAGAAATCCTTTATGATAAATCTGCTGATGGGAGCACTTATGTTACAGGACTTTCCATGTCAAAGGCTACTGATAAGAAAATTGTGGAAGCTGATGCTTATGTTGCTGCCTGTGATGTCCCTGGAATTAAAAGATTAATCCCACCAGAGTGGAGGGAAAAGGAGTTGTTCAATAACATATACGAACTTGTTGGAGTTCCTGTGGTCACAGTGCAACTCAGATACAATGGTTGGGTTACAGAATTGCAGGATCTAGAACTGTCAAGGCAACTGAGGAAAGCTACTGGGTTAGATAACCTTCTCTATACTCCGGATGCAGATTTTTCTTGCTTTGCAGACCTTGCACTTGCGTCTCCTGAAGACTATTACATTGAGGGACAAGGATCGTTGCTCCAATGTGTTCTGACGCCCGGAGATCCATACATGCCCTTGCCAAATGAAGAAATTATTTCAAGAGTAGCAAAACAGGTCATATCACTGTTCCCATCATCTCAAGGTTTAGAAGTGACTTGGTCATCTGTTGTTAAAATTGGCCAATCTCTGTATCGTGAGGGACCTGGCAAAGATCCATTTAGACCTGACCAAAAGACTCCAGTGAAGAATTTCTTTCTTTCTGGCTCTTACACAAAACAGGATTACATAGACAGCATGGAAGGCGCAACCTTATCCGGCAGGCAAACCTCAGCTTATATCTGTGATGCGGGGGAAGAATTGGTGGCATTGCGGAAGGAGCTTGTTGCACAGTCTAAAGACGACATTAAATATACAAACACTAAAGATGAATTGAGTTTAGTATGAGAGAAATATTCAGATAAGCTAGTCACGTTATATGAAGAAAGCTTGTTTTCTATGCATGTAATATTTTATGTTGTATATTTAATCAAAAAGCAATTACAAGATTGTTAGTATTGCAGCAAGAAGTGCTGTTTATACTTGTGAATTATGGTGTTTATAAATTGTGATTCGTACTCAGTGGTAGACATTCTTAATAATGAAGTAATGTTGGAG
TARGET=1801,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2504
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.216250
PRIMER_LEFT_SEQUENCE=GGCAAAGATCCATTTAGACCTG
PRIMER_RIGHT_SEQUENCE=ATATAAGCTGAGGTTTGCCTGC
PRIMER_LEFT=1756,22
PRIMER_RIGHT=1889,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.784
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.2504
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.216250
PRIMER_LEFT_1_SEQUENCE=CTGGCAAAGATCCATTTAGACC
PRIMER_RIGHT_1_SEQUENCE=ATATAAGCTGAGGTTTGCCTGC
PRIMER_LEFT_1=1754,22
PRIMER_RIGHT_1=1889,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.784
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.3273
PRIMER_LEFT_2_PENALTY=0.111077
PRIMER_RIGHT_2_PENALTY=0.216250
PRIMER_LEFT_2_SEQUENCE=TCACTGTTCCCATCATCTCAAG
PRIMER_RIGHT_2_SEQUENCE=ATATAAGCTGAGGTTTGCCTGC
PRIMER_LEFT_2=1672,22
PRIMER_RIGHT_2=1889,22
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=59.784
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=0.3538
PRIMER_LEFT_3_PENALTY=0.137575
PRIMER_RIGHT_3_PENALTY=0.216250
PRIMER_LEFT_3_SEQUENCE=ACTGTTCCCATCATCTCAAGGT
PRIMER_RIGHT_3_SEQUENCE=ATATAAGCTGAGGTTTGCCTGC
PRIMER_LEFT_3=1674,22
PRIMER_RIGHT_3=1889,22
PRIMER_LEFT_3_TM=59.862
PRIMER_RIGHT_3_TM=59.784
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=216
PRIMER_PAIR_PENALTY_4=0.4378
PRIMER_LEFT_4_PENALTY=0.221594
PRIMER_RIGHT_4_PENALTY=0.216250
PRIMER_LEFT_4_SEQUENCE=AGACCTGACCAAAAGACTCCAG
PRIMER_RIGHT_4_SEQUENCE=ATATAAGCTGAGGTTTGCCTGC
PRIMER_LEFT_4=1771,22
PRIMER_RIGHT_4=1889,22
PRIMER_LEFT_4_TM=59.778
PRIMER_RIGHT_4_TM=59.784
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_04687 Ps_contig_mira-and-tgicl-ass_franssen_34269
SEQUENCE=ACTTACTTCAATTTCAACTCCACCGCTCCATAACCTCTCTCTCTCTCTCTCAATAAATACTCCATTACTTCAACTTTAACCTTGACCTCACTCTCTCTTCCAAATGGCCGAAAATAACAACTCAAACCCTACTCCTCCTCCACCCTCCTCACCACCTCTCACACCGTCGCATCAACTAACCGATTCATCCACCCTAACTCCCCATCCAGAACCACAACCGGAACTCCCCGTTACCGACCAAAAAGAACTCTCCGATGCACCACCGCAACCAACCGAATCAGAAACCGATTCAAAAGGTAACCCGTCACAAGATGTAGTAGCAGTTGAACCCTCACCAGAACCAGAACAACAACAAGTTTCAATCTACGGCGTTCCGTTACTCCAAGACGAACGAACCGATGTAATCCTCCTCAAATTCCTCCGAGCACGCGACTTCAAAGTGAAAGACTCACACACAATGATCACCAACACGCTTCAATGGAGGAAAGATTTCAACATAGACGCACTTCTGGACGAAGATCTAGGGGATGACTTAGACAAAGTTGTATTCATGCATGGCTTTTCAAGAGAGGCACATCCTGTTTGTTATAATGTCTATGGCGAGTTTCAGAATAAGGAACTTTATGATAAGACTTTTGGGAATGAGGAAAGGAGGAACACGTTTTTGCGTTGGAGGATTCAGTTTTTGGAGAAGAGTATTAGGAAACTTGATTTTTATCCTGGTGGTGTTAACACTGTTTTTCAAGTTAATGATCTTAAAAATTCTCCGGGTCCGGCTAAAAGAGAACTTAGACTTGCTACTAAACAGGCTTTGCAGTTGCTTCAGGATAATTATCCCGAGTTTGTTGCTAAACAGGTTTTTATCAATGTGCCATGGTGGTATCTTGCATTCTATACCATGATCAATCCGTTCTTGACTCAGAGGACCAAGAGTAAATTTGTGTTTGCAGGCCCATCGAAGTCTCCCGACACGCTTTTCAAGTATATTTCTCCTGAGCAAGTGCCAGTTCAATATGGTGGCCTGAGTGTTGATTTTTGTGATTGCAACCCGGATTTCAGCATCAATGATCCCACCACGGAAATTCCTGTAAAGCCGACAACTAAGCAAACTGTGGAAATTGCTATATATGAGAAATGCATTATTGTCTGGGAGTTACGCGTGGTGGGCTGGGAAGTTAGTTATAGTGCTGAATTCAAACCCGATGCTGAGGATAGATATGCAGTTATTATACAGAAGGCTACTAAGATGGCCCCTAGCGACGAACCAGTAGTTTCCAACAGCTTTAAAGTCAGTGAACTAGGGAAATTGTTCCTCACTGTCGACAATCCTACCTTGAAAAAGAAGAGGCTTCTTTACAGGTTCAAGATCAAACCCTACTCTGATTGAGAGAAAGTCGCTCTAGTCTCTCGAGTCTTATGATTTTCGATATATGAAGGAACTAGAGCTCGGTGAGTAAGAAGTATGTGTGTCATAATTTGGTATTCATCTATAGTTTTGGTCTTGAGTTGTTTGCATATGGTGTTGTCTGTTTTTTATTGTATTTAATATTTTATTTATTGCTAGAAAACATTATATAGCTAGTCTAAATTTGTTGATTTTAATTTTATTTTATTTTATTTTCTGGTGTTGAACAATGTCAAGGGGGAGAGTAGTAGCACATGGAATTGGAAATGATGTTGGTTTGAGATTATGTGTTTAAGCAGAATTATCTTATATGATAGAAATAGTTTATGCATAAGCACTTATATGATAAATGATTATGTTAGAAACGCTTAATTAACTAGTTTATTCAAACATCACATTCTTTGTGCCCTATAAC
TARGET=35,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9442
PRIMER_LEFT_PENALTY=0.869127
PRIMER_RIGHT_PENALTY=0.075027
PRIMER_LEFT_SEQUENCE=TACTTCAATTTCAACTCCACCG
PRIMER_RIGHT_SEQUENCE=GTTAGGGTGGATGAATCGGTTA
PRIMER_LEFT=3,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.131
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.9786
PRIMER_LEFT_1_PENALTY=0.869127
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=TACTTCAATTTCAACTCCACCG
PRIMER_RIGHT_1_SEQUENCE=CATCGGAGAGTTCTTTTTGGTC
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=256,22
PRIMER_LEFT_1_TM=59.131
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=254
PRIMER_PAIR_PENALTY_2=0.9849
PRIMER_LEFT_2_PENALTY=0.869127
PRIMER_RIGHT_2_PENALTY=0.115779
PRIMER_LEFT_2_SEQUENCE=TACTTCAATTTCAACTCCACCG
PRIMER_RIGHT_2_SEQUENCE=GGTTTGAGTTGTTATTTTCGGC
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=127,22
PRIMER_LEFT_2_TM=59.131
PRIMER_RIGHT_2_TM=59.884
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=1.0659
PRIMER_LEFT_3_PENALTY=0.869127
PRIMER_RIGHT_3_PENALTY=0.196811
PRIMER_LEFT_3_SEQUENCE=TACTTCAATTTCAACTCCACCG
PRIMER_RIGHT_3_SEQUENCE=TTAGGGTGGATGAATCGGTTAG
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=196,22
PRIMER_LEFT_3_TM=59.131
PRIMER_RIGHT_3_TM=60.197
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=1.1081
PRIMER_LEFT_4_PENALTY=0.869127
PRIMER_RIGHT_4_PENALTY=0.238974
PRIMER_LEFT_4_SEQUENCE=TACTTCAATTTCAACTCCACCG
PRIMER_RIGHT_4_SEQUENCE=CCATTTGGAAGAGAGAGTGAGG
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=106,22
PRIMER_LEFT_4_TM=59.131
PRIMER_RIGHT_4_TM=60.239
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=104
=
PRIMER_SEQUENCE_ID=SSR_04688 Ps_contig_mira-and-tgicl-ass_franssen_34269
SEQUENCE=ACTTACTTCAATTTCAACTCCACCGCTCCATAACCTCTCTCTCTCTCTCTCAATAAATACTCCATTACTTCAACTTTAACCTTGACCTCACTCTCTCTTCCAAATGGCCGAAAATAACAACTCAAACCCTACTCCTCCTCCACCCTCCTCACCACCTCTCACACCGTCGCATCAACTAACCGATTCATCCACCCTAACTCCCCATCCAGAACCACAACCGGAACTCCCCGTTACCGACCAAAAAGAACTCTCCGATGCACCACCGCAACCAACCGAATCAGAAACCGATTCAAAAGGTAACCCGTCACAAGATGTAGTAGCAGTTGAACCCTCACCAGAACCAGAACAACAACAAGTTTCAATCTACGGCGTTCCGTTACTCCAAGACGAACGAACCGATGTAATCCTCCTCAAATTCCTCCGAGCACGCGACTTCAAAGTGAAAGACTCACACACAATGATCACCAACACGCTTCAATGGAGGAAAGATTTCAACATAGACGCACTTCTGGACGAAGATCTAGGGGATGACTTAGACAAAGTTGTATTCATGCATGGCTTTTCAAGAGAGGCACATCCTGTTTGTTATAATGTCTATGGCGAGTTTCAGAATAAGGAACTTTATGATAAGACTTTTGGGAATGAGGAAAGGAGGAACACGTTTTTGCGTTGGAGGATTCAGTTTTTGGAGAAGAGTATTAGGAAACTTGATTTTTATCCTGGTGGTGTTAACACTGTTTTTCAAGTTAATGATCTTAAAAATTCTCCGGGTCCGGCTAAAAGAGAACTTAGACTTGCTACTAAACAGGCTTTGCAGTTGCTTCAGGATAATTATCCCGAGTTTGTTGCTAAACAGGTTTTTATCAATGTGCCATGGTGGTATCTTGCATTCTATACCATGATCAATCCGTTCTTGACTCAGAGGACCAAGAGTAAATTTGTGTTTGCAGGCCCATCGAAGTCTCCCGACACGCTTTTCAAGTATATTTCTCCTGAGCAAGTGCCAGTTCAATATGGTGGCCTGAGTGTTGATTTTTGTGATTGCAACCCGGATTTCAGCATCAATGATCCCACCACGGAAATTCCTGTAAAGCCGACAACTAAGCAAACTGTGGAAATTGCTATATATGAGAAATGCATTATTGTCTGGGAGTTACGCGTGGTGGGCTGGGAAGTTAGTTATAGTGCTGAATTCAAACCCGATGCTGAGGATAGATATGCAGTTATTATACAGAAGGCTACTAAGATGGCCCCTAGCGACGAACCAGTAGTTTCCAACAGCTTTAAAGTCAGTGAACTAGGGAAATTGTTCCTCACTGTCGACAATCCTACCTTGAAAAAGAAGAGGCTTCTTTACAGGTTCAAGATCAAACCCTACTCTGATTGAGAGAAAGTCGCTCTAGTCTCTCGAGTCTTATGATTTTCGATATATGAAGGAACTAGAGCTCGGTGAGTAAGAAGTATGTGTGTCATAATTTGGTATTCATCTATAGTTTTGGTCTTGAGTTGTTTGCATATGGTGTTGTCTGTTTTTTATTGTATTTAATATTTTATTTATTGCTAGAAAACATTATATAGCTAGTCTAAATTTGTTGATTTTAATTTTATTTTATTTTATTTTCTGGTGTTGAACAATGTCAAGGGGGAGAGTAGTAGCACATGGAATTGGAAATGATGTTGGTTTGAGATTATGTGTTTAAGCAGAATTATCTTATATGATAGAAATAGTTTATGCATAAGCACTTATATGATAAATGATTATGTTAGAAACGCTTAATTAACTAGTTTATTCAAACATCACATTCTTTGTGCCCTATAAC
TARGET=1602,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2290
PRIMER_LEFT_PENALTY=0.093026
PRIMER_RIGHT_PENALTY=0.136017
PRIMER_LEFT_SEQUENCE=ATGAAGGAACTAGAGCTCGGTG
PRIMER_RIGHT_SEQUENCE=TACTCTCCCCCTTGACATTGTT
PRIMER_LEFT=1430,22
PRIMER_RIGHT=1651,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=59.864
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.3200
PRIMER_LEFT_1_PENALTY=0.093026
PRIMER_RIGHT_1_PENALTY=0.226971
PRIMER_LEFT_1_SEQUENCE=ATGAAGGAACTAGAGCTCGGTG
PRIMER_RIGHT_1_SEQUENCE=TGCTACTACTCTCCCCCTTGAC
PRIMER_LEFT_1=1430,22
PRIMER_RIGHT_1=1657,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=59.773
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=0.3200
PRIMER_LEFT_2_PENALTY=0.093026
PRIMER_RIGHT_2_PENALTY=0.226971
PRIMER_LEFT_2_SEQUENCE=ATGAAGGAACTAGAGCTCGGTG
PRIMER_RIGHT_2_SEQUENCE=GTGCTACTACTCTCCCCCTTGA
PRIMER_LEFT_2=1430,22
PRIMER_RIGHT_2=1658,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=59.773
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=0.3200
PRIMER_LEFT_3_PENALTY=0.093026
PRIMER_RIGHT_3_PENALTY=0.226971
PRIMER_LEFT_3_SEQUENCE=ATGAAGGAACTAGAGCTCGGTG
PRIMER_RIGHT_3_SEQUENCE=GCTACTACTCTCCCCCTTGACA
PRIMER_LEFT_3=1430,22
PRIMER_RIGHT_3=1656,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=59.773
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.4328
PRIMER_LEFT_4_PENALTY=0.093026
PRIMER_RIGHT_4_PENALTY=0.339815
PRIMER_LEFT_4_SEQUENCE=ATGAAGGAACTAGAGCTCGGTG
PRIMER_RIGHT_4_SEQUENCE=CATGTGCTACTACTCTCCCCCT
PRIMER_LEFT_4=1430,22
PRIMER_RIGHT_4=1661,22
PRIMER_LEFT_4_TM=59.907
PRIMER_RIGHT_4_TM=59.660
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_04689 Ps_contig_mira-and-tgicl-ass_franssen_34277
SEQUENCE=CTTATAAAAAAATGAAGATTTCTCCTAGTTATACAAAAGTTAACAAAATTATCTACAACAATCTTAAATCATCTACATGATAAGGAGCAGAACAATCAAAACTGCATTTTTATATTGTCACATATCATATAGTAAGTTAGCTTCAACAAATTGCATTTTCATCACTCAAGGAAACATCACTAGGCTTCTCAGAAGAGGCAATGTAATGATGATATTCATTTACTTGTATAGCAAGAAACAATCAATTCGTCCCAAATGCACGTTTCGCCGAATCAAGTGTGTTCGAGAGAAGCATTGAAATTGTAACTGGTCCTACGCCTCCGGGAACTGGTGTAATGGCCGATGCCACCTTGACCGCTTCGTCGAAGCAAACATCTCCCGTGACACGAAAGACTCCGCTGTTGGAATCCTTAACTTGATTTGTTCCCATGTCAATAACCACAGCTCCTTTCTTTATCCAATCGCCACGAACTATGTTCGCGATTCCAACATCCGTGACGATAATGTCCGCTTCAGACGTTATCTGTTCAGGATTCTTCGTGTACGCGTGCAACAAACTGACAGTTGCATGGTGCCTCTGCAATAGCAATGAAGTTGGTAATCCTGCAATTTTACTTCTTCCAATTATCGCAACTCTTTTCCCTCTGATTTCGACACCATGTCTAAGTAACAACTCAATGCAGCTCTTTGAGGCACAAGGTATAAAGAAGGGTTTTCTTCCTCGAATTGCAAGGTTTCCTATATTTAAAGGATGAAATCCATCGACGTCCTTTTCAGGACGAATAACATTCATAATTCTTTCCTCGTCGAGATGCTGAGGTAAAGGGAGTTGCACAACAATCCCATGCACATCATCATCATCATTGAAACCAGAAACAACATCAAGCAGTTCATTTTCAGTACAACTCTCTGGCAACTCAGAAACCACAGTTCCAATTCCAACTTTATCACAAGCTTTCATCTTAACATGAATGAAAGTATGAGAATCCTTTCTATCACCTACCAAAACCACAGCCAATCTAGGAAACTTCCCAATCTCACTCTTCATCTTTCTTATCTCATCAGCCACTTTCAGCTTTATCTGTTTAGCAATTGGTTTACCTTCAATGATTCTAGCACTATGACCATCATTAGACCATTGGAAACTTTGTGGCAAAAGGGTATGAGGGGAAGTGTTGTTCTCTTTAGGTATCCAAACGTCAGGAAGATTAGGGCCAAGAAAAGTACAAGAAGGTGGAGAAGAAAAAGAATGAAAATGCAATGTTTTTCTGAGAGTTTTATGCCATGGTGTTGTCTACNNNNRANGAGCGAAAATGTAAAGATTTTTTCATGTTTGTTTGTGAAAATTAGTGTATTTTTGGTGACCCCCGCGTACTCTGCGTTGA
TARGET=850,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04690 Ps_contig_mira-and-tgicl-ass_franssen_34286
SEQUENCE=TAAAAAATAATATTCCCTTCTCTTTTCCTCATCGTCTAGGGTTCCCAAATCAGAAACAACCCCAGTTTTTCATTCTCTCTGAACCGGAACGGAATGGAAGCTTTTCTGTCGGATCCCGAACCGGTTCGGGTCAAGAGGAAAACTCTTCAAGCCGTCTTGGAACAGTGTCAGCGAGCCCTTGAACTCATCAATAACGCTTCCGATCAAGACGACGGCGATGTTTCTGATTCTAATGATGAAAGTCTCCCTTCTACACCACCCGATCCAGAAGCCGATCAGTTGTGTGACTTGCTTAAATCAAGACTTGAGTGCCCTGATTTCCTTGAGAAGTTAGAGTGTGCACAGGCGTCGGTTTATCAGAACACAGTTGAAGAAGGTAGTTCTTGGGATTTGGTCAGTGAAAATGATCTTTGGGAAGGTGTTGACTCTGATGAAGAAGATTATGTTCTTGTTAGACAAGATGATATAGTGGATGGAATTGCTTGCTTTATGGCTGCCTATTTGTTGTCTCTTAAGAAAACCAAGGATTTGACTCCAAATCAACTCCAGGATGCACTCAGCAAGACATTTTCGGTGAAAAAGAAAAAGGGAAAGCTTCGAAAGGCCTGGGATGGGAGCAAAGTTATTTATAATGTTGCATCATGGGGGGCTACAGCCATAGGGATATATCAAAATCCTGTTATTCTAAGGGCTGCAACTAAAGCATTCTGGACTTCTTGTCAAGTGATATCAAAGCTAATCTGAGTTGGCAATGCAGCTGGGAGGAACATTGTGATATAGTAATTTAACTGTTTACTTTGCTTTGCTTGCTGCTGCAATTCAGTTTTATGTACAGTTCAATTTGTTTGCACTACAGATCAATTGATCTTTAATGTAGTTCGATTCGATTTGATTTCGATCTTGTAAATATCTCTATTCAAATGTTCCGAGTGCAAATAAGATTCACTATTTCAAGCCCTRGNAACATAACCAATGTACTTCGATTTGATTTGATTTCGATCTTGTAAATATCTCTATT
TARGET=982,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04691 Ps_contig_mira-and-tgicl-ass_franssen_34323
SEQUENCE=GGCAATGCAAAACAGATAACCATTCTAAAAATTCACAATTTTAACATTACTGAGCTACAAATACAAATAATAAACAGAAGTTTGACTGTAATAGTAATGGAAGTCTTCTTCCACTGTGTGAAATATTGAATCTACTATACATCACATGTAATGTAATACTTTTTATTTCCATTAGTGTTTCATGAAAGATTACAAGAATGCCATATAATTTATCTTCTCGCTTCGTTTTGTACCAAAATATCATAACAGCTGAACTGCTGCGATGGCAGGTATTTTTTCTCGCAATTTTTGTGTAACAGCAACACGCACAGTTATGACACCGGCAGCTGGACCCGTGATTCGAACCTTTACTATTGGCTCCTCAATGGCTACCAATTCAAGAGATCCACCAGCTGCCCCAACCAGATATGGCCTTATTTCCTCTAATACCTTCTCAATATTTTCATCATTTAGCTCAAGTCCAGTCTCTTCGTCAGATATTGGTTCAACTGCGACTATTTCAGGGATCTTTTCCATTAATCGCCGTTCAATGCCCAATTTCATTGTCGTAACAGAACTCGGACATGATCCACAAGCACCTTGTAACTTTAACCGTACAACATTGCCATCGATTTCATGTAAAGCAACATTTCCACCGTCAGAAATGAGATATGGTCGAATTTCGTCGAGCACAGTTTCTACATTTTCGGCAGTTAATGGCAATTCCACAGCTGGATCTGGAGCGGCAACGGCCTTTATAGCAAATGGACGTGTAGAGTGCGGTGAATGAAACCAAACGCAAGGACGCAGAGAGTTATTGTTCGTCCTCCTAAAAGGAACATGCTTACCGAAAAACCTTGTACCCTTTTCGGTGTTGGAGGAAGAAGAAGGAGAAGATTGAAGGGATCTACAGTAAGATTGTGTATTGAGCACCACACCTTGCATCGGTGTGTTGATATTTTGACCTAACAGAGCTAATCAATCAAAATGTTCGAAATTATTCTTCTCTGTTTGTTTGTTTTCTTCTTCTCTCACCACCCAAACACCATCGCAATTCGCAGCAAATTCCGTTTCTGCCTCTCCCCGAC
TARGET=988,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1629
PRIMER_LEFT_PENALTY=0.017127
PRIMER_RIGHT_PENALTY=0.145769
PRIMER_LEFT_SEQUENCE=CCTAAAAGGAACATGCTTACCG
PRIMER_RIGHT_SEQUENCE=GTTTGGGTGGTGAGAGAAGAAG
PRIMER_LEFT=807,22
PRIMER_RIGHT=1022,22
PRIMER_LEFT_TM=60.017
PRIMER_RIGHT_TM=60.146
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=0.2567
PRIMER_LEFT_1_PENALTY=0.110942
PRIMER_RIGHT_1_PENALTY=0.145769
PRIMER_LEFT_1_SEQUENCE=CTTGCATCGGTGTGTTGATATT
PRIMER_RIGHT_1_SEQUENCE=GTTTGGGTGGTGAGAGAAGAAG
PRIMER_LEFT_1=917,22
PRIMER_RIGHT_1=1022,22
PRIMER_LEFT_1_TM=59.889
PRIMER_RIGHT_1_TM=60.146
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=0.2859
PRIMER_LEFT_2_PENALTY=0.140098
PRIMER_RIGHT_2_PENALTY=0.145769
PRIMER_LEFT_2_SEQUENCE=ACCGAAAAACCTTGTACCCTTT
PRIMER_RIGHT_2_SEQUENCE=GTTTGGGTGGTGAGAGAAGAAG
PRIMER_LEFT_2=825,22
PRIMER_RIGHT_2=1022,22
PRIMER_LEFT_2_TM=60.140
PRIMER_RIGHT_2_TM=60.146
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=0.2859
PRIMER_LEFT_3_PENALTY=0.140098
PRIMER_RIGHT_3_PENALTY=0.145769
PRIMER_LEFT_3_SEQUENCE=AAAACCTTGTACCCTTTTCGGT
PRIMER_RIGHT_3_SEQUENCE=GTTTGGGTGGTGAGAGAAGAAG
PRIMER_LEFT_3=830,22
PRIMER_RIGHT_3=1022,22
PRIMER_LEFT_3_TM=60.140
PRIMER_RIGHT_3_TM=60.146
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.3214
PRIMER_LEFT_4_PENALTY=0.175676
PRIMER_RIGHT_4_PENALTY=0.145769
PRIMER_LEFT_4_SEQUENCE=GTGTAGAGTGCGGTGAATGAAA
PRIMER_RIGHT_4_SEQUENCE=GTTTGGGTGGTGAGAGAAGAAG
PRIMER_LEFT_4=749,22
PRIMER_RIGHT_4=1022,22
PRIMER_LEFT_4_TM=60.176
PRIMER_RIGHT_4_TM=60.146
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_04692 Ps_contig_mira-and-tgicl-ass_franssen_34324
SEQUENCE=TTTTCGTTCGTATATTATAAGCCGAGGACGAGCCAGTTCATTGTAAGTGTGAACAAGTACTTGTCAGGTATCAATAATAAGTTCGCTGTCGGCATGCGGTTCAAGATGAGGTTTGAGAGTGATGATTCTGCCGAGTCTGACAAAAGATTCTCCGGTACCATAGTTGGAGTCGAAGATATCTCTCCCCATTGGGCAAACTCAAAATGGCGATCACTCAAGGTTCAATGGGATGAGCCTGCGGCTATTCCAAGACCCGACAGGGTTTCGCCATGGGACATAGAACCCTTTGTTTCTTCAGCTTTGACACCAACAGTTCAACCTACGGCTGCAAAGACAAAGCGGCCTCGACCAACCAATGAAACTCCTGATGTCGACACGACATCGGCCGCCTCTATTTTCTGGGATGCTAGGCTGGCACAGACCGATATGACACAACGTAGCGTTTTAGCCGAAAGCAAAAAAGGCGATAATAATATTAGTACGTGGCATCATAAGCAAACCGATACGATGAATGCTAAAAAGCAGCAACAACAACAACAACAATGCTTTATTGAGGAATCATACAGAAGGAAGTTGGCTATCGTCTCCCCGTTCCAGCCATCCTTCTCATTCGCTTCACGACACAACCGATGATAGCAAGACCCTCTCAGCTTGGCCTGTTTCACAGTCTCAATCATCTATACTGAACGTTGATCGCGTCCCTGATCAAGTTGATAAAGACAACAAAGTCGAGACAGCTACGGGTTGTCGATTGTTTGGAGTCGAACTTATTGATCATTCGAAAAACTCGGGTGCTGTAGGGAAAACATCTTCACATGCAGTAAATGTAACCGGAGCAACCACGGAAGTTAGTTCCAGCACCATGTCTAGCATTGATACCGGCCGTAAGTCTGACATCTCAAAGGCTTATACGGAGAAGGAAACAAGAACCGCAACAAGTATCACCGAAGGAAACTCAGAACAAGCAAATTTGTAGTAGAAGTCGAACCAAGGTTCAAATGCAGGGCGTTGCAGTTGGCCGAGCGGTGGATTTGGCAATGTTGAACGGGTATGATCAACTTATCGATGAATTAGAAGAGTTGTTCGACATCAAGGGACAGCTTCAACACAGAAACAAGTGGGAAATTGTCTTCACCGACAACGAAGGAGATATGATGCTTGTCGGAGACGATCCATGGCCTGAGTTCTGTAACATGGTAAAAAGAATCTTCATTTGTTCAAGCCAAGATGTGAAGAAGATGAAATCAGGAAGCAAATTACCAATCTCTTCTTCGATCGAAGAAGGGACCGTGATAAGCTCAGACACAACCGAACGATAAAACATCGACATTATTTTCTTGTCCTTTTACCTTTTTTCTTTGTTTTCTCAGCACCAAACCGTTAATGTTATTGTTGAAGTTAGTTTTATTTTTTAGCTAGGTGAGTTTTTCATGTTATTATTATTTAGATGTGAAACACTAGCTAATTTTTATAACTTAATTTGTAGARGAAACACAAGACAAGG
TARGET=526,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04693 Ps_contig_mira-and-tgicl-ass_franssen_34327
SEQUENCE=GGGGACTCTTTTTNATCGCTGAACCAAACAGGGTAAATTCTCTCTCTCCAAAACCATTTACTCCAATCACACAACATTCGGTTCAGCGTTCACAGCGATTGAATGGGTCTTCTCACCATCATTAGAAAAATCAAAAAGAAGGAAAAGGAAATGCGAATTCTTATGGTTGGGTTGGACAATTCTGGAAAAACTACAATCGTTTTGAAGATTAATGGGGAGGACACTAGTGTCATTAGTCCTACCCTTGGCTTCAACATCAAAACCATCGCCTACCAGAAGTACACTCTAAATATATGGGATGTTGGAGGCCAAAAAACAATTCGATCTTACTGGAGAAACTACTTTGAGCAAACAGATGGTTTAGTTTGGGTAGTTGACAGTTCAGATCTTAGAAGGTTGGATGATTGCAAAATGGAGCTAGATAACCTTCTAAAGGAAGAGAGACTATCTGGAGCATCCTTACTAATTCTAGCAAATAAACAAGATATTAAAGGTGCCCTTGCACCCGATGAAATAGCTAAGGTGTTGAACTTAGAAACTATGGATAAGTCTAGGCACTGGATGATAGTTGGCTGTAGTGCATACACCGGTGAGGGTTTGCTTGAGGGATTTGATTGGCTGGTCCAGGACATAGCATCTAGGATCTATGTGCTCGACTAATTATGGTCATGTCAATATCCGTGAAAGGGTTCAAAGTTCAAACTTTGAATTTAAGAGCATTCTTAATCCCAATATGCTATCTTAAGACATCGTTATGCATCTCTAAGATGTTTCACATGGAATTTTTCCGTTGATGTGTACAATAACNTTTAACGTTTGAAACCCATAGGGAAAATGGCTTCTTTGTGCCTACNTACTT
TARGET=39,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5441
PRIMER_LEFT_PENALTY=1.524309
PRIMER_RIGHT_PENALTY=0.019805
PRIMER_LEFT_SEQUENCE=TTTNATCGCTGAACCAAACAG
PRIMER_RIGHT_SEQUENCE=AATTGTCCAACCCAACCATAAG
PRIMER_LEFT=10,21
PRIMER_RIGHT=180,22
PRIMER_LEFT_TM=59.476
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.50
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.75
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=1.5455
PRIMER_LEFT_1_PENALTY=1.524309
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=TTTNATCGCTGAACCAAACAG
PRIMER_RIGHT_1_SEQUENCE=TACTTCTGGTAGGCGATGGTTT
PRIMER_LEFT_1=10,21
PRIMER_RIGHT_1=280,22
PRIMER_LEFT_1_TM=59.476
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.50
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=1.6466
PRIMER_LEFT_2_PENALTY=1.524309
PRIMER_RIGHT_2_PENALTY=0.122257
PRIMER_LEFT_2_SEQUENCE=TTTNATCGCTGAACCAAACAG
PRIMER_RIGHT_2_SEQUENCE=TTGAAGCCAAGGGTAGGACTAA
PRIMER_LEFT_2=10,21
PRIMER_RIGHT_2=253,22
PRIMER_LEFT_2_TM=59.476
PRIMER_RIGHT_2_TM=60.122
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.50
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=1.6865
PRIMER_LEFT_3_PENALTY=1.524309
PRIMER_RIGHT_3_PENALTY=0.162220
PRIMER_LEFT_3_SEQUENCE=TTTNATCGCTGAACCAAACAG
PRIMER_RIGHT_3_SEQUENCE=CTCCCCATTAATCTTCAAAACG
PRIMER_LEFT_3=10,21
PRIMER_RIGHT_3=218,22
PRIMER_LEFT_3_TM=59.476
PRIMER_RIGHT_3_TM=59.838
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.50
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=1.7113
PRIMER_LEFT_4_PENALTY=1.524309
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=TTTNATCGCTGAACCAAACAG
PRIMER_RIGHT_4_SEQUENCE=GTGTCCTCCCCATTAATCTTCA
PRIMER_LEFT_4=10,21
PRIMER_RIGHT_4=223,22
PRIMER_LEFT_4_TM=59.476
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.50
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_04694 Ps_contig_mira-and-tgicl-ass_franssen_34332
SEQUENCE=GTTAGATTTAGTCGTCGCAATTTTCTATCGCCAACCACATNAAATTRGAGGCTTGAATGTGTTTGTTTTGTTAGACCATATTTTCAATGCTTCTTCTGCCAATGAAAAAAACTCTTGATCCACAATTAGAACTGATGCATTACAGTGACTTAGAAGAAAAGCTACTGTGGAGGCATTTAGCCTAATATTCACTGTGTTTAACACTGACCCTGCCATTGGAACTGCAAAATGAGCTTCATAAATAGCTGGAACATTAGGCGCAATAACAGATACAGTGTTTCCAAGTCCGATGGAAAAGGTAGAAAGAGCAGAAGCGAATCGACGGCAACGTTGATACGTCTGATGCCATGTGTAATGACGAGATCCATGGATGACTGACTTTCTGTTTGGATGAACCTTAGCTGCTCTTTCCAAAAACCATAATGGCGTTAATGCTGTATGGTTTGCTGCGTTCTTTGGCAATTCATCAATGTCTCGTGCAGCCATCGCCATTGCTCTCTCTCTCTCTCTAATGTCCACAGGACTCAGAACAATTGTCTTTTTTAATGTGAAATTTTTAGACAT
TARGET=495,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04695 Ps_contig_mira-and-tgicl-ass_franssen_34337
SEQUENCE=ATGTGTACACATGTTCTCATGCCATGTGTGTTTGTCCCTNGAAGAAGAAGTAAAAGCTACCAACGCAGAGTACGCGGGGAGAAGATAGATAGATAGATATCTCTTTCTATTCTCTCTTCTCCCCCCAAAACCAAGAAATTCTCAAACCCTAATTTTCAATTTTGTTTCCCAGGCCCAAATGTCTCATACCTCTCAAACCCTAATGCCTCTTGATTCCGAAGAACCACCTTCGTCAACCCCAATTCCCTCTCCCCCTAATCCATCCCCAAATTCTACTTCCACCGCTCTTCATATCTCCTTCAACCAAGACTCCGGTTGTTTCGCCGTTGGCACCGATCAAGGCTTCCGAATCTATAATTGTGACCCCTTTCGTGAAATATTCCGACGTGATTTCGGTCACGGCGGAGGAATCGGGTTGGTTCACATGTTGTTTCGATGCAATATTCTTGCTTTCGTTGGCGGGGGATCGGACCCTCGGTATCCGGCTGATAGGGTGATGATCTGGGATGATCATCAGTCGCGGTGTATTGGAGAGTTGTCGTTTCGATCGGAGGTGAAAGGGATTCGTCTGAGGAGGGATAGAATTGTTGTTGTTTTGGGGCATAAGATTTTTGTTTATAATTTCTCTGATCTTAAGGTTCTTCATCAAATTGAGACTATTGCGAACCCTAAGGGTCTCTGTGAGGTTTCACATCATTTGGAAACTATGGTGTTGGCTTGTCCTGGGTTGCAGAAAGGTCAGATTAGGGTTGAACATTATGCTTCCAAGAGAACCAAGTTCATCCTGGCACATGATTCTAGAATTGCTTGTTTCAATATAACCCAAGATGGACTTTTACTTGCTACTGCAAGCTCCAAGGGGACTCTCATTAGGATTTTCAATACCCTGGATGGTTCTTTGCTCCAAGAGGTACGGAGAGGTGCTGACCGAGCTGAGATATATAGCCTTGCCTTTTCTCCCACGGCTCAGTGGTTAGCTGTCTCGAGTGACAAGGGAACTGTTCATGTATTCAACCTGAAGGTTGATTCGGGATTGTTGGGGCACGAGAGATCACATACTACATCTGAGTCTAGTCCTATTAGTCCATCGGCAGCTTTGTCTCTCTCATTTATTAGAGGTGTATTGCCTAAGTATTTTAGCTCAGAATGGTCCGTGGCTCAATTTCGCTTGCAAGAAGGTTTACAGTATAATGTTGCCTTTGGCCATCAGAAAAATACAATTGTGATACTCGGCATGGATGGCAGTTTCTATCGGTGTCAGTTTGATTCTGCAGCCGGTGGGGAGATGACACAACTTGAATATTACAATTTCCTAAAGCCAGAAGAGACATTCTAGTATATGCTTGCATGAGTACATGATTATGTATATATCTTTGTTGGCACTTGCTGTGAATACCATTTGCATTTCTATTCCCTGCCTTATTTATAATAACTTTATTGTAAATAGAGGTTATGCAAAGCCACTAATGCCATCCAATTCCCTCTGTTGCCGTGTACAGCATATATTTAAGCTCGGTTATGTCAATCCAAATTATTGTCCCGAGGTTTACAGTCC
TARGET=83,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0691
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=TGTGTTTGTCCCTNGAAGAAGA
PRIMER_RIGHT_SEQUENCE=GAGAGGTATGAGACATTTGGGC
PRIMER_LEFT=26,22
PRIMER_RIGHT=193,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.50
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.50
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=11.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.0714
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=TGTGTTTGTCCCTNGAAGAAGA
PRIMER_RIGHT_1_SEQUENCE=ATCAAGAGGCATTAGGGTTTGA
PRIMER_LEFT_1=26,22
PRIMER_RIGHT_1=213,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.50
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.50
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.75
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=188
PRIMER_PAIR_PENALTY_2=0.2455
PRIMER_LEFT_2_PENALTY=0.213725
PRIMER_RIGHT_2_PENALTY=0.031769
PRIMER_LEFT_2_SEQUENCE=ATGTGTGTTTGTCCCTNGAAGA
PRIMER_RIGHT_2_SEQUENCE=GAGAGGTATGAGACATTTGGGC
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=193,22
PRIMER_LEFT_2_TM=59.786
PRIMER_RIGHT_2_TM=59.968
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.50
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.50
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=11.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.2478
PRIMER_LEFT_3_PENALTY=0.213725
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=ATGTGTGTTTGTCCCTNGAAGA
PRIMER_RIGHT_3_SEQUENCE=ATCAAGAGGCATTAGGGTTTGA
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=213,22
PRIMER_LEFT_3_TM=59.786
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.50
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.50
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.75
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=0.2539
PRIMER_LEFT_4_PENALTY=0.037339
PRIMER_RIGHT_4_PENALTY=0.216588
PRIMER_LEFT_4_SEQUENCE=TGTGTTTGTCCCTNGAAGAAGA
PRIMER_RIGHT_4_SEQUENCE=AGAGCGGTGGAAGTAGAATTTG
PRIMER_LEFT_4=26,22
PRIMER_RIGHT_4=287,22
PRIMER_LEFT_4_TM=60.037
PRIMER_RIGHT_4_TM=59.783
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.50
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.50
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_04696 Ps_contig_mira-and-tgicl-ass_franssen_34338
SEQUENCE=TTTACTTCCCCCAGCATGAAAAGAAATAAGAAAAGACCGCCAAAAATAGTATCAAAATTTTARCTTGTCTTCGATGCCAATCTTACTGCCTTCCCTTCAATACAAAAATTCTTACTACCTGCGAGCTTTTGCTTCAAGTAAGCAATAGCCTCTTTTTTAGAAGAATACTCGGACAAAACCGAAGATGAATGTTGATCAACAAGAACTTTGAGCTGTTTCAGTTTCAATGATTTTCCAGGCACCTGTTTTAAAATTTGTTTACACAACTTCTTCAGCTTCAATTTGGGTTCAACAATCTTTTCTACCTCATCCTCTGTTTCGTTTTCCTGAGAATCTATGTCACCGGAATCTTCATCATCACTATTACCAAATGACGTCTTTTTACCCTCGAAATAGCATCCAGCTACTTTTTTGGGCCTGTCTTTGATGCCTAATCCTTGCTTTCCTGTTGTGGCTTTATCTTGGACTAAGTTGTAGAGGTTTTCTTGATCTTGTTCAAAAAAAGGGGTTCTCGCCATCCCAGCTTTAGCACTTTCAGATGTAGAGGACTTTCTCTTCTTTGATGCTGCACCGAGTAATCCACCAGAGACAAACCCAAACTTTAAGCCCCACCACTCTGGATGGATATCAGGACATTTGCTTTCTTCAATTTCAAAATCATGAGTTTCAGATGTCTTTGACAAATCCAATTCATCCTCAGCATTGTCAATACTGTCAGATATGTCACCTTTTTTAACCAGAATTCCTTCAAGGTCCTTTAAGGAATATTGAGAGACACGCTTTCCTCTCTCTCTTTTCTGGTATCTTCCTTGTGGTCTAGTAGACCTTGATACAGAAACAGGAGGATCAACTGTAACAGCCTGATCGGCCTCGGTTTCCACCTCAGTAGTGTTACTCTCTGTGTCATTTGATACAGGCGCTTGAACTTTCAATCGCTTGAGTATGTTATCAAATTGTGTAGTATCAAATGCCCACTGGTTTGGCTTCTCTAATCCAATACCAGTAGTGTCTTGTTTGTTGGTGACACGAACATGTCCTTTGATCCCTTGCTTATCTTTTCCAAGACCTTCACCTTCTTCCCATCCCATTTGCTTCATGAGATTGAAAGCGGCGGATCTTCTGGAGACACCCACGTAACACAGAGGAGTTTCGGGTGCCGCCATGGCTCCTCCGATTGATTGCACTGCCGCCGGTTTTTCTCGTCTTCGTGTTCTTTGGTAACGCGATGGATGCTGTGTTGGTTTCNAATCTTGCTGTTAG
TARGET=785,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04697 Ps_contig_mira-and-tgicl-ass_franssen_34340
SEQUENCE=AGTACGTCGGGGAGTGAGAATTATACCACAAAATAAAGTGTTGTTATCCGAAACTCAAACTGAGAGTGAATAAGGAGAGAAAATGGTGAATCTAGCATCAGCTTCCGTGCAATTCTCAAACCCATTTCCGCCATCTTCTTCTTACAAACCTCTTATCCGCAGGAACTTCCTTCTCTTCACCACAAGAGCCAACGAACCTCAAACACCACCCTCTGATTCGGACCCATCCAAACCCGATTCCGAGATAACCGCCACCGGAGATGACTTTGATGACCGCATTGACCAACTCCGCCGCCGTAACCGGAGGGGCACTGGCAAGAAGGCGGAAGTCCGTAAGAGCCGAAAGGCGAATAAGGCTACTTCAGGGTCAGCGCCGGGGTCCGGCGTGTACCTACCGCCGGTGGCGTTAAAAGAACCGGTTTCCGGTGGATTGAAGGTGGAGTTGGGGTTTAGCAAATTCAGCGAGAGATTGAACGGTCGTGTTGCGATACTTGGATTGACGGCACTGTTGCTGGTGGAATTGGCAACAGGGAAAAGCGTTATTAATTACCATACACCTGGCACTGTACTTATTCAGTTTTATTTTATGGCTGCACTTACTGCTATCTATGTCAAGTTTCAGAAGGAGAAAATCAGCATTTGGCCTGACTCCTCCTCAACAAACTAGTGTTTTTTTATCGTTGAGCTTCAGTTTGTTATATATTATTATTATTACTATTATTGTTCTTGAGCTTCAATTTGTACAATAGAAAAAAAATACTATTGCTTCTATATGCTTTCAAATAGTTATAGGCGATCTTCATTTGTTATACAGTTAACTGAACCAAATGTTATTTTTACTGATAATAATGTGATTTCAAACTTAAAATTTTANCCTAC
TARGET=701,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04698 Ps_contig_mira-and-tgicl-ass_franssen_34341
SEQUENCE=TTTTCATTGTACAAATTGAAGCTCAACAACAATAATAGTAATAATAATAATATATAACAAATTGAAGCTCAACGAATAAAAAAAACACTAGTTTGTTGAGGAAGAGTCGGGCCAAATGCTGATTTTCTCCTTCTGAAACTTGACATAGATAGCAGTAAGGGCAGCCATAAAATAAAACTGAATAAGTACAGTGCCAGGTGTATGGAAATTAATAACGCTTTTCCCTGTTGCCAATTCCACCAGCAACAGTGCCGTCAATCCAAGTATCGCAACACGACCGTTCAATCTCTCGCTGAATTTGCTAAACCCTAACTCCACCTTCAATCCACCGGAAACCGGTTCTTTTAATGCCACCGGCGGTAGGTACACGCCGGACCCCGGCGCTGACCCTGAAGTAGCCTTATTCGCCTTTCGGCTCTTACGGACTTCCGCCTTCTTGCCAGTGCCCCTCCGGTTACGGCGGCGGAGTTGGTCAATGCGGTCATCAAAGTCACCTCCGGTGGCGGTTATCTCGGAATCGGGTTTGGATGGGTCCGAATCAGAGGGTGGTGTTTGAGGTTCGTTGGCTCTTGTGGTGAAGAGAAGGAAGTTCCTGCGGATAAGAGGTTTGTAAGAAGAAGATGGCGGAAATGGGTTTGAGAATTGCACGGAAGCTGATGCTAGATTCACCATTTTCTCTCCTTATTCACTCTCAGTTYGTAGTTTCGGATAACAACACTTTTA
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04699 Ps_contig_mira-and-tgicl-ass_franssen_34343
SEQUENCE=AAGCAGTGTATCAACGCAAGAAGTACGCGGGGATCATGTGGTATGCTATTCTATCCTATTCTCCTTAAATCCACTTCATTATACACAACTTCTTCAAACTTCAAACACAATCAAACTATCTTAAACGATGTCGTTTCCCTGCGACTACTGCGACACAAGAAACGCGGTTCTATACTGCAGACCAGACTCCGCGAAACTCTGCTTAGTTTGCGACCAGCACGTGCACTCTGCTAACGCACTCGCGCTCAAGCACGTGCGTTATCAGATTTGTCAAAACTGTAAAAACGACGCCGCTTCGGTTCGTTGCTTCACCGATAACCTAGTTCAATGCCTCCGCTGTGATTGGGACGCTCACGGCGGTGATTCTTCTTCTTCTTCTTCTTCCCATCACCATAACCACAACCGCCGCCGTCTGGAAGGTCTCACCGGATGTCCTTCCGTTCACGAGATTGTCATTGCGTTAGGGCTTGATTTGAAGCCGAACGACGCGGTTTTTGTGGCGGATTTTGAAGGTCCTGTTGTTCCGGTTGTTCACAATAGGGATGAGATTTATGAACAGGTGGCGGAAGTTGCAAAGAGAAAGAGGAATTTGGAAGCTGAAAATGATTGTAGATTTAGTGATTGTAGCAATGAAGTTGATGATTTGTTGTTGCTTCAGCAAACTCCGTTTACTTCTTTGCTCAATTTTTCATCTGAATTCGATGTCGGCGCTAAGAAAATTAATAACGATTATGGAAATGAATCTGGTGTTCTTCTCTGGAATCGTAACCCTACTTATCAGCCTCCTCAGGTCTGGGATTTTCAATTACAGAAATCTAGAGATATGACATATGATGGTGTAGAAGTAGTTCCAAAATCATTGCAGGATGTGCATTACTCAACACTTGGTGATGATTTTTCTATCAAGAAGTAATCATTCAGATCAGTCATCATCAAGCCATGTGAAGAAGAAAGTAGATAGCAACAAGAAAACTAGAGATGGGTTACCAACTGAGTCCAAATTAGTTGAATCCATAACATACAATGGTGCCGCCGACAATGTACCAGTTATGGAGTATCTTTTGAGTGGGAGTGAAAATGTTAGTAACATAAATGGCAAGGTTAGCTTGGAAGAACAAGTAAGGAATAGAGGGGATGCCATGTTACGCTATAAGGAGAAGAAGAAAACTCGAAGGTTTGACAAGCACATTCGATATGAATCAAGGAAGGCTAGGGCTGATACGAGAAAGAGAGTGAGAGGGAGATTTGTTAAGGCAGGTGATGATGACATTCAAGAAGGATGAGTGCCTTCTCAGAAACCTCTCATCCCATGGCATTGGCCTTGTACGTGTATACGTATATTATATATAAATATCAGAGAAATAATAATAGGTTCTAGTTTTAATGAGGAATTCCTTCGGTATTGATTTANTTATATCATTCTTTCTTGCC
TARGET=364,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5789
PRIMER_LEFT_PENALTY=0.477034
PRIMER_RIGHT_PENALTY=0.101896
PRIMER_LEFT_SEQUENCE=CCGATAACCTAGTTCAATGCCT
PRIMER_RIGHT_SEQUENCE=CTATTGTGAACAACCGGAACAA
PRIMER_LEFT=311,22
PRIMER_RIGHT=539,22
PRIMER_LEFT_TM=59.523
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.5789
PRIMER_LEFT_1_PENALTY=0.477034
PRIMER_RIGHT_1_PENALTY=0.101896
PRIMER_LEFT_1_SEQUENCE=ATAACCTAGTTCAATGCCTCCG
PRIMER_RIGHT_1_SEQUENCE=CTATTGTGAACAACCGGAACAA
PRIMER_LEFT_1=314,22
PRIMER_RIGHT_1=539,22
PRIMER_LEFT_1_TM=59.523
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.6417
PRIMER_LEFT_2_PENALTY=0.477034
PRIMER_RIGHT_2_PENALTY=0.164639
PRIMER_LEFT_2_SEQUENCE=CCGATAACCTAGTTCAATGCCT
PRIMER_RIGHT_2_SEQUENCE=TCAGCTTCCAAATTCCTCTTTC
PRIMER_LEFT_2=311,22
PRIMER_RIGHT_2=599,22
PRIMER_LEFT_2_TM=59.523
PRIMER_RIGHT_2_TM=59.835
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.6417
PRIMER_LEFT_3_PENALTY=0.477034
PRIMER_RIGHT_3_PENALTY=0.164639
PRIMER_LEFT_3_SEQUENCE=ATAACCTAGTTCAATGCCTCCG
PRIMER_RIGHT_3_SEQUENCE=TCAGCTTCCAAATTCCTCTTTC
PRIMER_LEFT_3=314,22
PRIMER_RIGHT_3=599,22
PRIMER_LEFT_3_TM=59.523
PRIMER_RIGHT_3_TM=59.835
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=0.6655
PRIMER_LEFT_4_PENALTY=0.563643
PRIMER_RIGHT_4_PENALTY=0.101896
PRIMER_LEFT_4_SEQUENCE=CACGTGCGTTATCAGATTTGTC
PRIMER_RIGHT_4_SEQUENCE=CTATTGTGAACAACCGGAACAA
PRIMER_LEFT_4=250,22
PRIMER_RIGHT_4=539,22
PRIMER_LEFT_4_TM=60.564
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_04700 Ps_contig_mira-and-tgicl-ass_franssen_34345
SEQUENCE=CTTGTCATATGAGTATATAAAAAGGCTGTAGTTTCCCGCCAGCATGGTATCGACGCAGTGTGTGTGTGAGAGAGTGAAGAAGATGTTGTGGGTGGACAAGTATCGTCCCAAAACCCTAGACCATGTTATGGTTCACAGTGATACCGCTCAAAATCTCAAGAAATTGGTTTCGGAACATGATTGTCCCCATTTGCTGTTCTATGGTCCATCAGGTTCTGGCAAGAAAACCCTAATCATGGCTCTTCTCCGTCAAATGTTTGGACCCGGTGCCGAAAAGGTGAAGGTGGAAAATAGGGCATGGAAAGTGGATGCCGGGAGTAGATCTATTGATTTAGAGCTGACAACATTATCAAGTGCCAATCATATCGAAATGACTCCGAGCGATGCAGGCTTTCAGGATAGATACATTGTGCAGGAAATAATCAAAGAAATGGCTAAAAATAGACCAATTGATACTAAAGGAAAGAAAGGATTTAAAGTGCTTGTCCTTAATGATGTGGACAAACTCTCTAGAGAAGCCCAGCATTCTCTCCGCAGAACAATGGAGAAATATAGCGCTTATTGCAGATTGATTTTATGTTGCAATAGTTCTTCAAGAGTCACAGAAGCCATCCGCTCCCGTTGTCTTAATGTACGAGTAAATGCACCAAGTGAAGAACAGATTGTTGACGTTTTACAGTTCATTGGTAAAAAAGAAGGGCTGCAACTTCCTTCTAGTTTTGCCGCTCGCATAGCAGAGAAATCTAATCGGAACTTGAGGAGGGCCATATTATCATTTGAGACTTGCCGCGTCCAACAGTATCCTTTCACTGACAAACAAACAATTTCCCCAATGGACTGGGAGGAATATGTTTCAGAAATTGCATCCGACATAATGAAGGAACAGAGCCCAAAAAGGTTGTTTCAAGTTCGAGGAAAACTGTATGAGCTTCTAATCAATTGCATTCCTCCCGAAATCATTTTGAAGAGGCTACTTTATGAGCTATTGAGAAAACTCGATGCTGAACTTAAGCATGAAGTCTGCCATTGGGCAGGATATTATGAACATAGGATGCGCCTTGGACAGAAGGCCTATTTTTCACTATTGAAGCTATTTGTGGCCAAGTTCATGAGCGTTACTAATTAAACTTCCCTTCCTCTATTGACCTAAACTACGTTTCCGGGCTTAGGAAAACGTTATTTAGGAAAGG
TARGET=58,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2624
PRIMER_LEFT_PENALTY=1.242619
PRIMER_RIGHT_PENALTY=0.019805
PRIMER_LEFT_SEQUENCE=ATAAAAAGGCTGTAGTTTCCCG
PRIMER_RIGHT_SEQUENCE=GTTCCGAAACCAATTTCTTGAG
PRIMER_LEFT=16,22
PRIMER_RIGHT=175,22
PRIMER_LEFT_TM=58.757
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=1.3448
PRIMER_LEFT_1_PENALTY=1.242619
PRIMER_RIGHT_1_PENALTY=0.102139
PRIMER_LEFT_1_SEQUENCE=ATAAAAAGGCTGTAGTTTCCCG
PRIMER_RIGHT_1_SEQUENCE=TAACATGGTCTAGGGTTTTGGG
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=127,22
PRIMER_LEFT_1_TM=58.757
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=112
PRIMER_PAIR_PENALTY_2=1.3749
PRIMER_LEFT_2_PENALTY=1.242619
PRIMER_RIGHT_2_PENALTY=0.132262
PRIMER_LEFT_2_SEQUENCE=ATAAAAAGGCTGTAGTTTCCCG
PRIMER_RIGHT_2_SEQUENCE=CTGATGGACCATAGAACAGCAA
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=58.757
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=1.4711
PRIMER_LEFT_3_PENALTY=1.242619
PRIMER_RIGHT_3_PENALTY=0.228464
PRIMER_LEFT_3_SEQUENCE=ATAAAAAGGCTGTAGTTTCCCG
PRIMER_RIGHT_3_SEQUENCE=GACGGAGAAGAGCCATGATTAG
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=250,22
PRIMER_LEFT_3_TM=58.757
PRIMER_RIGHT_3_TM=60.228
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=1.4721
PRIMER_LEFT_4_PENALTY=1.242619
PRIMER_RIGHT_4_PENALTY=0.229480
PRIMER_LEFT_4_SEQUENCE=ATAAAAAGGCTGTAGTTTCCCG
PRIMER_RIGHT_4_SEQUENCE=TAGGGTTTTGGGACGATACTTG
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=117,22
PRIMER_LEFT_4_TM=58.757
PRIMER_RIGHT_4_TM=60.229
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=102
=
PRIMER_SEQUENCE_ID=SSR_04701 Ps_contig_mira-and-tgicl-ass_franssen_34345
SEQUENCE=CTTGTCATATGAGTATATAAAAAGGCTGTAGTTTCCCGCCAGCATGGTATCGACGCAGTGTGTGTGTGAGAGAGTGAAGAAGATGTTGTGGGTGGACAAGTATCGTCCCAAAACCCTAGACCATGTTATGGTTCACAGTGATACCGCTCAAAATCTCAAGAAATTGGTTTCGGAACATGATTGTCCCCATTTGCTGTTCTATGGTCCATCAGGTTCTGGCAAGAAAACCCTAATCATGGCTCTTCTCCGTCAAATGTTTGGACCCGGTGCCGAAAAGGTGAAGGTGGAAAATAGGGCATGGAAAGTGGATGCCGGGAGTAGATCTATTGATTTAGAGCTGACAACATTATCAAGTGCCAATCATATCGAAATGACTCCGAGCGATGCAGGCTTTCAGGATAGATACATTGTGCAGGAAATAATCAAAGAAATGGCTAAAAATAGACCAATTGATACTAAAGGAAAGAAAGGATTTAAAGTGCTTGTCCTTAATGATGTGGACAAACTCTCTAGAGAAGCCCAGCATTCTCTCCGCAGAACAATGGAGAAATATAGCGCTTATTGCAGATTGATTTTATGTTGCAATAGTTCTTCAAGAGTCACAGAAGCCATCCGCTCCCGTTGTCTTAATGTACGAGTAAATGCACCAAGTGAAGAACAGATTGTTGACGTTTTACAGTTCATTGGTAAAAAAGAAGGGCTGCAACTTCCTTCTAGTTTTGCCGCTCGCATAGCAGAGAAATCTAATCGGAACTTGAGGAGGGCCATATTATCATTTGAGACTTGCCGCGTCCAACAGTATCCTTTCACTGACAAACAAACAATTTCCCCAATGGACTGGGAGGAATATGTTTCAGAAATTGCATCCGACATAATGAAGGAACAGAGCCCAAAAAGGTTGTTTCAAGTTCGAGGAAAACTGTATGAGCTTCTAATCAATTGCATTCCTCCCGAAATCATTTTGAAGAGGCTACTTTATGAGCTATTGAGAAAACTCGATGCTGAACTTAAGCATGAAGTCTGCCATTGGGCAGGATATTATGAACATAGGATGCGCCTTGGACAGAAGGCCTATTTTTCACTATTGAAGCTATTTGTGGCCAAGTTCATGAGCGTTACTAATTAAACTTCCCTTCCTCTATTGACCTAAACTACGTTTCCGGGCTTAGGAAAACGTTATTTAGGAAAGG
TARGET=813,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2453
PRIMER_LEFT_PENALTY=0.170871
PRIMER_RIGHT_PENALTY=0.074466
PRIMER_LEFT_SEQUENCE=TCTTCAAGAGTCACAGAAGCCA
PRIMER_RIGHT_SEQUENCE=ACATATTCCTCCCAGTCCATTG
PRIMER_LEFT=589,22
PRIMER_RIGHT=851,22
PRIMER_LEFT_TM=60.171
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.2678
PRIMER_LEFT_1_PENALTY=0.193337
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=CGAGTAAATGCACCAAGTGAAG
PRIMER_RIGHT_1_SEQUENCE=ACATATTCCTCCCAGTCCATTG
PRIMER_LEFT_1=634,22
PRIMER_RIGHT_1=851,22
PRIMER_LEFT_1_TM=59.807
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=218
PRIMER_PAIR_PENALTY_2=0.2943
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.074466
PRIMER_LEFT_2_SEQUENCE=TGCACCAAGTGAAGAACAGATT
PRIMER_RIGHT_2_SEQUENCE=ACATATTCCTCCCAGTCCATTG
PRIMER_LEFT_2=642,22
PRIMER_RIGHT_2=851,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.2943
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=0.074466
PRIMER_LEFT_3_SEQUENCE=AATGCACCAAGTGAAGAACAGA
PRIMER_RIGHT_3_SEQUENCE=ACATATTCCTCCCAGTCCATTG
PRIMER_LEFT_3=640,22
PRIMER_RIGHT_3=851,22
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.2943
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=0.074466
PRIMER_LEFT_4_SEQUENCE=GCACCAAGTGAAGAACAGATTG
PRIMER_RIGHT_4_SEQUENCE=ACATATTCCTCCCAGTCCATTG
PRIMER_LEFT_4=643,22
PRIMER_RIGHT_4=851,22
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_04702 Ps_contig_mira-and-tgicl-ass_franssen_34347
SEQUENCE=ATATNACAAAGAATCCTAAGAACAAATGTGAATATCACTTTAGGTAGGAAAGTAACTTTCTCTATTTTCATTCAATATTGATGGTCTGAACTTAAAGGACATGTGAAACATTTGAACTCCAAACTTCAGTTCACATACAACATAAATTACATACAATTCTCCACATCTTATTTCACTAAGATCAAATACATTCACAACTTCACTAGGCATTACCATAGGATGTAGGGATCGCATGTCTGATGAAGAAGCCCGACACCACTGTAACAACAAGCAATCATCACCGACGAATTTCCCTCATGAAATGTCATCAAGGAAGGTGGACAGCAATAGCCCTTGAACAAACTCGCATCTTTTTTCTTTTTCTTCCTTATCCTTGACTGACAAGGTAGGTGTCTCCACTTTTTTTTTTGATAGAGTCTCATCCGAGCTTTTTCTAGATTGCCTTGTCCCGGTAGGAAAGTTGGGTTGACTTTGAGAACTGGAAAATTCATCAGCTACGGGCAATATAAACGATGACAACAATGGATCAGGGGCTGCATTAGACGATGACAATGTACATGAAGACCCACCCAAAAGGGATTGCAAATTTCCTTCTCGCAGCTCCTTCCTCAATAAAGACAGTGTTGAATAAGATCCACCTTTCCGTGATTTCCTTTTTCGCTGCATCTTAAGTATGCTTCCATGTTGTAGAGCAATGTGTGCAACCATATCAACCCCCACCCTCAATGCGCAAACAGGACAAACCCCATTTTTGGCTTCAACAGGATGCTCTTCATCAATGTGGCAGCACAATCCAACAATATCAAAATACTCAGAACAAAAGGGACATAAAAACTCCTCCTTGACATCATCATCATCATCGTCAATATCATTTTCATCAAAAGCATCCATAAACATATCTGATCGAGATTGAAGAGCGGAGAGATAGGATTTGGAGGAAGTGAGGGTGGAAAAGGAAAGAAGAGCGTTCCAAGGAGAAGAAGAATCAGAGTCCATGAATGAAATTGGATCTCAATCTGAAATGAAAGTGAAGATTGAAGATTGAAGATTGAGATTGAGGTGGTGCTGGTTGGTTCTGTTCTTCTTCGTAACTAATTTCTGAGACAGACACAGCGACCCCCCCGACGTTCCTTTCTCCTCTCCTTTCTTATAGACCCCCG
TARGET=846,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3274
PRIMER_LEFT_PENALTY=0.225403
PRIMER_RIGHT_PENALTY=0.102004
PRIMER_LEFT_SEQUENCE=TGCTTCCATGTTGTAGAGCAAT
PRIMER_RIGHT_SEQUENCE=CTCTTCTTTCCTTTTCCACCCT
PRIMER_LEFT=674,22
PRIMER_RIGHT=964,22
PRIMER_LEFT_TM=59.775
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.3274
PRIMER_LEFT_1_PENALTY=0.225403
PRIMER_RIGHT_1_PENALTY=0.102004
PRIMER_LEFT_1_SEQUENCE=ATGCTTCCATGTTGTAGAGCAA
PRIMER_RIGHT_1_SEQUENCE=CTCTTCTTTCCTTTTCCACCCT
PRIMER_LEFT_1=673,22
PRIMER_RIGHT_1=964,22
PRIMER_LEFT_1_TM=59.775
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.4820
PRIMER_LEFT_2_PENALTY=0.380020
PRIMER_RIGHT_2_PENALTY=0.102004
PRIMER_LEFT_2_SEQUENCE=AATGTGTGCAACCATATCAACC
PRIMER_RIGHT_2_SEQUENCE=CTCTTCTTTCCTTTTCCACCCT
PRIMER_LEFT_2=693,22
PRIMER_RIGHT_2=964,22
PRIMER_LEFT_2_TM=59.620
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.7591
PRIMER_LEFT_3_PENALTY=0.657094
PRIMER_RIGHT_3_PENALTY=0.102004
PRIMER_LEFT_3_SEQUENCE=CAGGATGCTCTTCATCAATGTG
PRIMER_RIGHT_3_SEQUENCE=CTCTTCTTTCCTTTTCCACCCT
PRIMER_LEFT_3=761,22
PRIMER_RIGHT_3=964,22
PRIMER_LEFT_3_TM=60.657
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.7608
PRIMER_LEFT_4_PENALTY=0.225403
PRIMER_RIGHT_4_PENALTY=0.535412
PRIMER_LEFT_4_SEQUENCE=TGCTTCCATGTTGTAGAGCAAT
PRIMER_RIGHT_4_SEQUENCE=AATCCTATCTCTCCGCTCTTCA
PRIMER_LEFT_4=674,22
PRIMER_RIGHT_4=931,22
PRIMER_LEFT_4_TM=59.775
PRIMER_RIGHT_4_TM=59.465
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_04703 Ps_contig_mira-and-tgicl-ass_franssen_34352
SEQUENCE=AAGCAGTGGTATACAACGCAGAGTTTACGTTCGGGGATATCACTAAGATGAAGAATTTTATTTGGACATTCATGTGTATCTGTCTGAGCCTAATTGCTACAAAATCTTTATCTCAGTTTCAGCCAAGGCTACCAGAGGGACAACAAGTTCCATGTTTCTTCATATTTGGTGACTCATTGGTTGATAATGGAAACAACAATGGAATCCTAACACTTGCTAGAGCCAACTATAGGCCTTATGGCATCGACTTCCCACAGGGTGCCACCGGTCGCTTCACCAACGGTCGAACCTATGTCGATGCATTAGCTCAACTTCTTGGTTTTCGAGCATATATTCCTCCATATTCAAGAGCAAGGGGTTTGGACCTTCTAAGAGGAGCCAATTTCGCATCAGGAGCAGCAGGCATCAGACAAGAAACAGGCAGTAACCTGGGTGCTCATACATCAATGGATGAGCAAGTAACTAACTTCGGAAACGCAGTGCAGCAGATGCGGAGATTCTTTAGAGGAGACAACGATTCAATGAATAGTTACCTGAACAAATGTATATACTATTCAGGAATGGCAAGCAATGATTACCTTAATAACTATTTCATGACGGATTTTTATTCGACTAACGCTCAATACACTCCTAAAGCTTTTGCTTCTGCCCTTCTTCAAGCTTACGCTCGTCAGTTAACTCAATTACATTCATTAGGTGCAAGAAAAGTGATTGTTGCAGCAGTTGGACAAATCGGGTGCATACCATACGAATTAGCGCGTTTCAATGGAAACAACAGCAGATGCAACGACAAGATCAACGACGCGATTATATATTTCAACTCAGGTCTTAAACAGTTAGTTCAGAATTTCAATGGAGGACAGCTTCCAGGATCAAAGTTTGTGTACTTGGATTTTTATCAAAGTAGTGAAGATCTAGCTGCAAATGGAAAATCATATGGATTTGATGTTGTAGACAAAGGATGCTGTGGTGTTGGTAAAAACAACGGACAAATCACTTGTCTTCCATTGCAACAACCATGTGAAGAACGTGGCAAGTATTTGTTTTGGGATGCTTTTCACCCTACTGAGATGGCTAATATTTTACTAGCAAAAGAATCATATACTTCACAATCTTATACTTCTCCTATTAATATTCAACAATTGGCTATGCTTTAAGAGTTGAATATGTTTTTCATATTGTTTGTTTGTTAGTGTTTATTCAGTGCAAATTTGATGTTGTCTTGATATAAGAATGTTTTCATGTTTCTTAAAGTTACTTTCTATGAGTATGAATTGATGTCATTGTGGTGTAATAGTGATAATTCAATAAATATGCTTTTGTCTAAATTNNAAAA
TARGET=1179,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04704 Ps_contig_mira-and-tgicl-ass_franssen_34353
SEQUENCE=AGTTAATATCANAACGTTTTTATTGTNGACAAAAACAAGAAGAACTATAAAATTAAGGAATCACAGTTACACCAAATTTCCCACCAAACACAGCTTATATTTCTAAAAAGTAACAACAAATACCACAAAACCAAACATAAATAACCAAAGAAACACTAAATTTCAACCAAAGGCGTTCAAATTTATGAAAAGCACTATGAATTAAAAAACTGCCTCGACAAGAGCGTGCTTCTTTTTGGGGTATCCAATTCCAAGTATATGGTTTAGGTACACTTTCCCATTCTTCACAGTAGCTGCAGTAGCCAAACGATGTCTAGGCCCAGAGAATGTCGCCACAACAGAAGCTGTATTCCACCCATCTAAACTCTCCACCAATCTCCCAGAAGGAGTACTACCAGCAACCACCACCTTATCAGGAGACAACAACTCCAAACCATCACCAAAATAAAGTGGCCCTCCAGTAACCTTTACGATCTTCACTTGGTCTCCGTTTTTCAAATCAATCTTAAACAAATTTCCACTAAAAGTGTGAATCACTATCAAGAATCCATCTGGGTGGTAAACAATCCCGTTGAGTCCAACAAATGTTTTATGCCATTCTTTTGGAGTAAAGAGTGGACTCGTAATGATTGATATAACATTCCCATCAACCCCAACTTTCCAAATTTTATTCCCTTTTACATCTGTCACATAAGCATTGCCTTCTGCATCAACCGCAACATCGTCCGCAAAGGATTTCCCATCACTAGGGCCAGAAAGTTGAGTAAGAAAGAGGCGTTTCCACGTAGAAAGATCATAAGCAACGAGAGAACCATAACGGTTTCCCAAAACGTCGGCGTTTACTACCAGAACCCGATTTCTACTCCGGTCAATCGTCAAACCCAACGACGCGTTTCCGGCTAGCTCTGATTCCTTCACTATTGATTCCTCTTCAAACACTGTTCCGGGAGATTCGTTCTCACCAGGTACGGGAATTTGACCTATGCCGCCTTCAAAGAAAGAGACTATGAAACGGTTGTTGACGGAATCCCACTTGGCGCATTCTCGGAACCAACCGTTGCTGTGGTAGTGGTAGACGTGAGTGGGTGGTTGAGATCGTTCTAGGGATATGATTATGCCGATGGGTATGGCGGTGAGTAGGAGTAGAATGGCTAGGAATTTTGGAGAACAAAGGCACGAAAGAGCCATGAGAGAGAAAAAGAAAGAAAGAGAGGTT
TARGET=1198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04705 Ps_contig_mira-and-tgicl-ass_franssen_34355
SEQUENCE=ATATAATTGGTTGTAAGGATTAGTGAGTGCGATGCAATATCATCCACCTATCTTAATATTCAAGCATCTAAGCTTTCTATCTCCAAGCCATGGAGCAAAAGGTTGCCATAATGAAGCTCAAGGTTGATCTTCAATGTAGAAAATGCTGTAAGAAAGTTAAGAAGATCCTTTGCAAATATTCACGTGAGTTTTTCAATAATATATTTTTTCTCAATAATATTTTTTTTCAAATCTTCTTAAGTTAATTTTTTTTGTTGTTGTTGCAATTTTGATGAATGAAAGAAATTCGAGATCAATTATATGATGAGAAGAATGGCATCGTAACCATACGGGTGGTATGTTGTAGTCCAGAGAAGGTAAGAGACAATATTTGTTGTCAAGGGGGTGGTACAATCAAGAGTATAGAAATAGTGCAACCACCAAAGCCCAAAGAGCAAGAAAAAAAGCCCGAGGCCGAGATAAAGCCCAAAGCCCAACCAGGCCCACCTATTGTTATTCAACCTAAACAAACAAAACCAAATCCAACACCAGCACCGGCTCCCGCCCTAGCCCCGGCT
TARGET=252,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4246
PRIMER_LEFT_PENALTY=0.364827
PRIMER_RIGHT_PENALTY=0.059762
PRIMER_LEFT_SEQUENCE=AAAGGTTGCCATAATGAAGCTC
PRIMER_RIGHT_SEQUENCE=CGATGCCATTCTTCTCATCATA
PRIMER_LEFT=97,22
PRIMER_RIGHT=320,22
PRIMER_LEFT_TM=59.635
PRIMER_RIGHT_TM=60.060
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.5024
PRIMER_LEFT_1_PENALTY=0.364827
PRIMER_RIGHT_1_PENALTY=0.137550
PRIMER_LEFT_1_SEQUENCE=AAAGGTTGCCATAATGAAGCTC
PRIMER_RIGHT_1_SEQUENCE=ATGGTTACGATGCCATTCTTCT
PRIMER_LEFT_1=97,22
PRIMER_RIGHT_1=327,22
PRIMER_LEFT_1_TM=59.635
PRIMER_RIGHT_1_TM=59.862
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.8849
PRIMER_LEFT_2_PENALTY=0.825088
PRIMER_RIGHT_2_PENALTY=0.059762
PRIMER_LEFT_2_SEQUENCE=CAAAAGGTTGCCATAATGAAGC
PRIMER_RIGHT_2_SEQUENCE=CGATGCCATTCTTCTCATCATA
PRIMER_LEFT_2=95,22
PRIMER_RIGHT_2=320,22
PRIMER_LEFT_2_TM=60.825
PRIMER_RIGHT_2_TM=60.060
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.8849
PRIMER_LEFT_3_PENALTY=0.825088
PRIMER_RIGHT_3_PENALTY=0.059762
PRIMER_LEFT_3_SEQUENCE=GCAAAAGGTTGCCATAATGAAG
PRIMER_RIGHT_3_SEQUENCE=CGATGCCATTCTTCTCATCATA
PRIMER_LEFT_3=94,22
PRIMER_RIGHT_3=320,22
PRIMER_LEFT_3_TM=60.825
PRIMER_RIGHT_3_TM=60.060
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.9626
PRIMER_LEFT_4_PENALTY=0.825088
PRIMER_RIGHT_4_PENALTY=0.137550
PRIMER_LEFT_4_SEQUENCE=GCAAAAGGTTGCCATAATGAAG
PRIMER_RIGHT_4_SEQUENCE=ATGGTTACGATGCCATTCTTCT
PRIMER_LEFT_4=94,22
PRIMER_RIGHT_4=327,22
PRIMER_LEFT_4_TM=60.825
PRIMER_RIGHT_4_TM=59.862
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_04706 Ps_contig_mira-and-tgicl-ass_franssen_34368
SEQUENCE=TTATGTGTAAAAAAATAGATTGAATGATGATTCTGCAGTGACCGGAAATGATTCCATCCCTTAAATGATTGATGATGATGTTGATTTATAGAGTGAGAGCGGTAATAGTGTTTTTCTAGTAGTGTAAGTGAAGAAGAAGAAAATGGAAAAGCAGAGAAAAAAGATGAATCATCGCCGAAGATACTTTGCCTAGCGGAAGGGTTATGGGAATGTGCTGTTTTAATATCGCCGTTCTTCTTCTGGGGCACTGCCATGGTGGCGATGAAAGAAGTGCTTCCTAAATACAGTCCTTTCTTCGTTTCGTCTTTTCGTCTCATTCCAGCTGGGTTCCTTCTCGTTGCTTTTGCTGCTTCCAGAGGAAGGCCTTTTCCCTCTGGCTTTAATGCTTGGCTTTCCATTTCGCTCTTTGCTCTCATAGATGCTGCCTGCTTTCAGGGATTTCTCGCAGAAGGGTTGGAGAAGACTTCAGCTGGTTTGGGCAGTGTTATTATTGATTCACAACCTTTGACAGTGGCTGTACTTGCAGCTTTGTTGTTTGGTGAGTCCATTGGAATTATTGGAGCTGCTGGGCTTGTACTTGGTGTCGTAGGACTTGTGTTACTGGAGTTACCTGCCTTATCATTTGATGGGAGCGACTTCTCACTGTGGGGAAGTGGGGAGTTGTGGATGCTTCTTGCAGCTCAGAGCATGGCAGTTGGCACTGTTATGGTTCGTTGGGTATCCAAGTACTCTGATCCTATCATGGCAACTGGATGGCATATGGTTATTGGCGGCCTCCCCCTTGTGGCATTCGCAATTCTTAATAATGACCCTGCTGTAAGTGGGAGTCTTAAAGAGTACAGTTCAAGTGATATATTGTCACTTCTCTACACATCCATTTTTGGAAGTGCTGTTAGCTATGGTGTGTTCTTTTACAGTGCAACTAAAGGTAGCTTGACCAAGCTCAGTTCACTTACATTTCTAACCCCAATGTTTGCCTCAATTTTCGGGTTTCTGTATCTTGGTGAGACCTTCTCACCTGTACAGCTTGTTGGGGCCAGTGTAACTGTAGCTGCAATATACATGGTTAACTTCAGAAACTCCTTAGAGTGAATTATGTAGTCAGTTTTCTACTGGTTCTTTCACGTAACCCAACGGAAAAATTGCCACGAACAAGTTGGGAATGCTTGTGCTTTTGGACATGGAGAGGAGAGGAGAGAAGTTTGAGTAACATGTGATCTTAAGCACTTGCTATCATGCTTTACAGAGTATATACAATTTTGATTAGTTGTTAATATTGGCCTCGAGCCCCTTCCTACATAATATATAGCAGAAAATTGTAAGTTATTAGAGGTGAATACAAGCCTTATATACAAGGGAAATATTGTTTCCATAGCTTACTGTTGCACTAGGACAGAATAAGAATGTATATATTATGACTTTTGTTATGGCAAGTTTTTGTTATATGATGAATTGTTGATCACACAAATGAAATATAAATCTCGGGGTAGAGCTGAATTTGTGATGTAGTGATGGTATTTTGGACATATTATTCCAATCTGTAATTAGACC
TARGET=130,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5171
PRIMER_LEFT_PENALTY=0.439061
PRIMER_RIGHT_PENALTY=0.078029
PRIMER_LEFT_SEQUENCE=GAATGATGATTCTGCAGTGACC
PRIMER_RIGHT_SEQUENCE=AGACGAAACGAAGAAAGGACTG
PRIMER_LEFT=21,22
PRIMER_RIGHT=305,22
PRIMER_LEFT_TM=59.561
PRIMER_RIGHT_TM=59.922
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.5548
PRIMER_LEFT_1_PENALTY=0.439061
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=GAATGATGATTCTGCAGTGACC
PRIMER_RIGHT_1_SEQUENCE=AAAACAGCACATTCCCATAACC
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=220,22
PRIMER_LEFT_1_TM=59.561
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.5548
PRIMER_LEFT_2_PENALTY=0.439061
PRIMER_RIGHT_2_PENALTY=0.115779
PRIMER_LEFT_2_SEQUENCE=GAATGATGATTCTGCAGTGACC
PRIMER_RIGHT_2_SEQUENCE=TTCCGCTAGGCAAAGTATCTTC
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=59.561
PRIMER_RIGHT_2_TM=59.884
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=0.6443
PRIMER_LEFT_3_PENALTY=0.439061
PRIMER_RIGHT_3_PENALTY=0.205226
PRIMER_LEFT_3_SEQUENCE=GAATGATGATTCTGCAGTGACC
PRIMER_RIGHT_3_SEQUENCE=GACGAAACGAAGAAAGGACTGT
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=304,22
PRIMER_LEFT_3_TM=59.561
PRIMER_RIGHT_3_TM=59.795
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.8204
PRIMER_LEFT_4_PENALTY=0.439061
PRIMER_RIGHT_4_PENALTY=0.381349
PRIMER_LEFT_4_SEQUENCE=GAATGATGATTCTGCAGTGACC
PRIMER_RIGHT_4_SEQUENCE=AACGGCGATATTAAAACAGCAC
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=232,22
PRIMER_LEFT_4_TM=59.561
PRIMER_RIGHT_4_TM=60.381
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_04707 Ps_contig_mira-and-tgicl-ass_franssen_34368
SEQUENCE=TTATGTGTAAAAAAATAGATTGAATGATGATTCTGCAGTGACCGGAAATGATTCCATCCCTTAAATGATTGATGATGATGTTGATTTATAGAGTGAGAGCGGTAATAGTGTTTTTCTAGTAGTGTAAGTGAAGAAGAAGAAAATGGAAAAGCAGAGAAAAAAGATGAATCATCGCCGAAGATACTTTGCCTAGCGGAAGGGTTATGGGAATGTGCTGTTTTAATATCGCCGTTCTTCTTCTGGGGCACTGCCATGGTGGCGATGAAAGAAGTGCTTCCTAAATACAGTCCTTTCTTCGTTTCGTCTTTTCGTCTCATTCCAGCTGGGTTCCTTCTCGTTGCTTTTGCTGCTTCCAGAGGAAGGCCTTTTCCCTCTGGCTTTAATGCTTGGCTTTCCATTTCGCTCTTTGCTCTCATAGATGCTGCCTGCTTTCAGGGATTTCTCGCAGAAGGGTTGGAGAAGACTTCAGCTGGTTTGGGCAGTGTTATTATTGATTCACAACCTTTGACAGTGGCTGTACTTGCAGCTTTGTTGTTTGGTGAGTCCATTGGAATTATTGGAGCTGCTGGGCTTGTACTTGGTGTCGTAGGACTTGTGTTACTGGAGTTACCTGCCTTATCATTTGATGGGAGCGACTTCTCACTGTGGGGAAGTGGGGAGTTGTGGATGCTTCTTGCAGCTCAGAGCATGGCAGTTGGCACTGTTATGGTTCGTTGGGTATCCAAGTACTCTGATCCTATCATGGCAACTGGATGGCATATGGTTATTGGCGGCCTCCCCCTTGTGGCATTCGCAATTCTTAATAATGACCCTGCTGTAAGTGGGAGTCTTAAAGAGTACAGTTCAAGTGATATATTGTCACTTCTCTACACATCCATTTTTGGAAGTGCTGTTAGCTATGGTGTGTTCTTTTACAGTGCAACTAAAGGTAGCTTGACCAAGCTCAGTTCACTTACATTTCTAACCCCAATGTTTGCCTCAATTTTCGGGTTTCTGTATCTTGGTGAGACCTTCTCACCTGTACAGCTTGTTGGGGCCAGTGTAACTGTAGCTGCAATATACATGGTTAACTTCAGAAACTCCTTAGAGTGAATTATGTAGTCAGTTTTCTACTGGTTCTTTCACGTAACCCAACGGAAAAATTGCCACGAACAAGTTGGGAATGCTTGTGCTTTTGGACATGGAGAGGAGAGGAGAGAAGTTTGAGTAACATGTGATCTTAAGCACTTGCTATCATGCTTTACAGAGTATATACAATTTTGATTAGTTGTTAATATTGGCCTCGAGCCCCTTCCTACATAATATATAGCAGAAAATTGTAAGTTATTAGAGGTGAATACAAGCCTTATATACAAGGGAAATATTGTTTCCATAGCTTACTGTTGCACTAGGACAGAATAAGAATGTATATATTATGACTTTTGTTATGGCAAGTTTTTGTTATATGATGAATTGTTGATCACACAAATGAAATATAAATCTCGGGGTAGAGCTGAATTTGTGATGTAGTGATGGTATTTTGGACATATTATTCCAATCTGTAATTAGACC
TARGET=1183,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3514
PRIMER_LEFT_PENALTY=0.347541
PRIMER_RIGHT_PENALTY=0.003820
PRIMER_LEFT_SEQUENCE=TCTCACCTGTACAGCTTGTTGG
PRIMER_RIGHT_SEQUENCE=GCTCGAGGCCAATATTAACAAC
PRIMER_LEFT=1012,22
PRIMER_RIGHT=1287,22
PRIMER_LEFT_TM=60.348
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.8057
PRIMER_LEFT_1_PENALTY=0.801901
PRIMER_RIGHT_1_PENALTY=0.003820
PRIMER_LEFT_1_SEQUENCE=ACTGGTTCTTTCACGTAACCCA
PRIMER_RIGHT_1_SEQUENCE=GCTCGAGGCCAATATTAACAAC
PRIMER_LEFT_1=1111,22
PRIMER_RIGHT_1=1287,22
PRIMER_LEFT_1_TM=60.802
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.8250
PRIMER_LEFT_2_PENALTY=0.821141
PRIMER_RIGHT_2_PENALTY=0.003820
PRIMER_LEFT_2_SEQUENCE=GTTGGGGCCAGTGTAACTGTAG
PRIMER_RIGHT_2_SEQUENCE=GCTCGAGGCCAATATTAACAAC
PRIMER_LEFT_2=1029,22
PRIMER_RIGHT_2=1287,22
PRIMER_LEFT_2_TM=60.821
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=1.0196
PRIMER_LEFT_3_PENALTY=1.015786
PRIMER_RIGHT_3_PENALTY=0.003820
PRIMER_LEFT_3_SEQUENCE=CTGGTTCTTTCACGTAACCCA
PRIMER_RIGHT_3_SEQUENCE=GCTCGAGGCCAATATTAACAAC
PRIMER_LEFT_3=1112,21
PRIMER_RIGHT_3=1287,22
PRIMER_LEFT_3_TM=60.016
PRIMER_RIGHT_3_TM=59.996
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=1.0415
PRIMER_LEFT_4_PENALTY=1.037717
PRIMER_RIGHT_4_PENALTY=0.003820
PRIMER_LEFT_4_SEQUENCE=TTGGGGCCAGTGTAACTGTAG
PRIMER_RIGHT_4_SEQUENCE=GCTCGAGGCCAATATTAACAAC
PRIMER_LEFT_4=1030,21
PRIMER_RIGHT_4=1287,22
PRIMER_LEFT_4_TM=60.038
PRIMER_RIGHT_4_TM=59.996
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_04708 Ps_contig_mira-and-tgicl-ass_franssen_34370
SEQUENCE=GTCAGATGCAGAAAATCTCATACCAATATAAATATATATCCTACTAAAACTAGATGAAACATTGTGACAAATTTCAAAACAACATCCAGCAATAGCACTAATGCAACTTCTAATTCAAGCAACAAACAACTCTGATATATATGAAACAACAAATTAAAATCCTAATCCATTGTATCAGAGTGGAGGAAACAACACATGCAAGTCTTTGCTGAATACTCAAACAAAACCAAGCATCTAATTGTCTAAGGATTCGCCTTCCATTGTCACTGACTGGGGGACCTACTGCGGGCTGATTCCCTGCCTTCACGGTTTGGACTTGGACTTCTGCTCAGCACTGGGCCTTTCTCTGGACTCGGACTACGGCTTCTTGCATCCCCATTATTGAGTGGAGAGCGTGAGTATGACCTCTCCCTGCTGTGGTGTGATCTTTCTCTACTGTGCCTTCTATCCTCAGGTGATAGAGATCTTGAATATTCCCTTCGTTTAGGAGGAGGGGAATCATAGTCACGGCTGCGGGAGTGAGTTCTATGACGAGGTGGAGACCTGGAATAGCGGGGTGAACGAGAATAACGTGGGGGAGTCCTCCTTCGATCAGAATACCGACCACTTCTCTCCCTGACCCTCATCTCAGTAGGCTTCTTTCGATTCTCCTCAGCAAAAACAACAGTCAGCTCCCTACCAAGAAGAACTTGACCATCCATATGATATTTAGCATCTGCAGCATCAGCAGGATCCACAAATTGGATAAAACCAAACCCACGGGGTTGTCCAGTGTAATAATCTTTGGGGAGATAAATGTCTTTGAGAGGACCAAATTGACCAAAGGGTCTACGAAGATCTTCAGGCCTACAGTCATGGCGAAGGTTACGAACAAGAAGACTGGTAGGTAGATCACTTTCACGGGGGGCGTAACGGCGACGGGGACTTGGACTGCGTCCTCCTCCTCCTCTCTCCCCCCCTCTCTCTCTCTCTCGTCGTCGGTCGGTNGGTCGGTACGGTACGGGTACGGNTAGGGTTACGGNTTTACGGGTTTACCGGTTTACCGGTTTACCGTTTCCGTTTACCGTTCCGTTACCGTTCCGTTACCCGTTACCCGTTTACCCG
TARGET=936,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1071
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.106310
PRIMER_LEFT_SEQUENCE=CGGGGTTGTCCAGTGTAATAAT
PRIMER_RIGHT_SEQUENCE=GACGACGAGAGAGAGAGAGAGG
PRIMER_LEFT=759,22
PRIMER_RIGHT=978,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.894
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=59.091
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.8000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.1299
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.106310
PRIMER_LEFT_1_SEQUENCE=GAGAGGACCAAATTGACCAAAG
PRIMER_RIGHT_1_SEQUENCE=GACGACGAGAGAGAGAGAGAGG
PRIMER_LEFT_1=803,22
PRIMER_RIGHT_1=978,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.894
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=59.091
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=0.1299
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.106310
PRIMER_LEFT_2_SEQUENCE=CTTTGAGAGGACCAAATTGACC
PRIMER_RIGHT_2_SEQUENCE=GACGACGAGAGAGAGAGAGAGG
PRIMER_LEFT_2=799,22
PRIMER_RIGHT_2=978,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=59.894
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=59.091
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.1522
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.151478
PRIMER_LEFT_3_SEQUENCE=CGGGGTTGTCCAGTGTAATAAT
PRIMER_RIGHT_3_SEQUENCE=TAAACCGGTAAACCCGTAAANC
PRIMER_LEFT_3=759,22
PRIMER_RIGHT_3=1041,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=7.6500
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.1750
PRIMER_LEFT_4_PENALTY=0.023556
PRIMER_RIGHT_4_PENALTY=0.151478
PRIMER_LEFT_4_SEQUENCE=GAGAGGACCAAATTGACCAAAG
PRIMER_RIGHT_4_SEQUENCE=TAAACCGGTAAACCCGTAAANC
PRIMER_LEFT_4=803,22
PRIMER_RIGHT_4=1041,22
PRIMER_LEFT_4_TM=59.976
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.50
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.6500
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_04709 Ps_contig_mira-and-tgicl-ass_franssen_34370
SEQUENCE=GTCAGATGCAGAAAATCTCATACCAATATAAATATATATCCTACTAAAACTAGATGAAACATTGTGACAAATTTCAAAACAACATCCAGCAATAGCACTAATGCAACTTCTAATTCAAGCAACAAACAACTCTGATATATATGAAACAACAAATTAAAATCCTAATCCATTGTATCAGAGTGGAGGAAACAACACATGCAAGTCTTTGCTGAATACTCAAACAAAACCAAGCATCTAATTGTCTAAGGATTCGCCTTCCATTGTCACTGACTGGGGGACCTACTGCGGGCTGATTCCCTGCCTTCACGGTTTGGACTTGGACTTCTGCTCAGCACTGGGCCTTTCTCTGGACTCGGACTACGGCTTCTTGCATCCCCATTATTGAGTGGAGAGCGTGAGTATGACCTCTCCCTGCTGTGGTGTGATCTTTCTCTACTGTGCCTTCTATCCTCAGGTGATAGAGATCTTGAATATTCCCTTCGTTTAGGAGGAGGGGAATCATAGTCACGGCTGCGGGAGTGAGTTCTATGACGAGGTGGAGACCTGGAATAGCGGGGTGAACGAGAATAACGTGGGGGAGTCCTCCTTCGATCAGAATACCGACCACTTCTCTCCCTGACCCTCATCTCAGTAGGCTTCTTTCGATTCTCCTCAGCAAAAACAACAGTCAGCTCCCTACCAAGAAGAACTTGACCATCCATATGATATTTAGCATCTGCAGCATCAGCAGGATCCACAAATTGGATAAAACCAAACCCACGGGGTTGTCCAGTGTAATAATCTTTGGGGAGATAAATGTCTTTGAGAGGACCAAATTGACCAAAGGGTCTACGAAGATCTTCAGGCCTACAGTCATGGCGAAGGTTACGAACAAGAAGACTGGTAGGTAGATCACTTTCACGGGGGGCGTAACGGCGACGGGGACTTGGACTGCGTCCTCCTCCTCCTCTCTCCCCCCCTCTCTCTCTCTCTCGTCGTCGGTCGGTNGGTCGGTACGGTACGGGTACGGNTAGGGTTACGGNTTTACGGGTTTACCGGTTTACCGGTTTACCGTTTCCGTTTACCGTTCCGTTACCGTTCCGTTACCCGTTACCCGTTTACCCG
TARGET=959,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1522
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.151478
PRIMER_LEFT_SEQUENCE=CGGGGTTGTCCAGTGTAATAAT
PRIMER_RIGHT_SEQUENCE=TAAACCGGTAAACCCGTAAANC
PRIMER_LEFT=759,22
PRIMER_RIGHT=1041,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.151
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=5.8000
PRIMER_RIGHT_END_STABILITY=7.6500
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.1750
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.151478
PRIMER_LEFT_1_SEQUENCE=GAGAGGACCAAATTGACCAAAG
PRIMER_RIGHT_1_SEQUENCE=TAAACCGGTAAACCCGTAAANC
PRIMER_LEFT_1=803,22
PRIMER_RIGHT_1=1041,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.6500
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.1750
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.151478
PRIMER_LEFT_2_SEQUENCE=CTTTGAGAGGACCAAATTGACC
PRIMER_RIGHT_2_SEQUENCE=TAAACCGGTAAACCCGTAAANC
PRIMER_LEFT_2=799,22
PRIMER_RIGHT_2=1041,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.151
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.6500
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=243
PRIMER_PAIR_PENALTY_3=0.2801
PRIMER_LEFT_3_PENALTY=0.128591
PRIMER_RIGHT_3_PENALTY=0.151478
PRIMER_LEFT_3_SEQUENCE=AATTGACCAAAGGGTCTACGAA
PRIMER_RIGHT_3_SEQUENCE=TAAACCGGTAAACCCGTAAANC
PRIMER_LEFT_3=813,22
PRIMER_RIGHT_3=1041,22
PRIMER_LEFT_3_TM=59.871
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.6500
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=0.2861
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.285317
PRIMER_LEFT_4_SEQUENCE=CGGGGTTGTCCAGTGTAATAAT
PRIMER_RIGHT_4_SEQUENCE=GTAAACCCGTAAANCCGTAACC
PRIMER_LEFT_4=759,22
PRIMER_RIGHT_4=1034,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.715
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_04710 Ps_contig_mira-and-tgicl-ass_franssen_34384
SEQUENCE=TTGCGTGTCTCAGAAGCAGTGGTATCAAACGCAGAGTAACGCAGGGGAAACATATCTTATTATCCATTCATTCATTTTTGCTTGCAAAGANACCCACACCAACCATCGCAGAACCAAAATGTCCTTCATCTCTGTTTGTTGCTGCAACGGTTTGGTAGCTCCACTGTCCAGTCCTATTAATCTTCAAGCTCCACTCCAACCACTCACACTGAAGAGAACCACAGTCATAGCTTCTGCTTCAAAATCTGGAGGAGGATTATCACTCAATTCGATATTCAAAAGTTGTAAGAATTGTGAAGGCAAAGGTGCTATTCAATGTCCAGGATGCAAGGGAACAGGAAAGAATAAAAAGAATGGAAATATATTTGAACGGTGGAAGTGCTTTGGGTGCCAAGGGTTTGGGCTAATCGGTTGCCCTAACTGTGGAAAGGGAGGGTTGACACCAGAACAAAGAGGAGAAAGATAAAGAAACTTTAAAAATAATCAATTTTCTTACCAGTGTGGAGAATTTGGAGATAGTGTAAAATTACATGCAAAAGGAGAAAAAGAGATTAAAGCAACTGAAGTGTTTGGTGTGGGTTTATTTACTTGAAGTTCATTCATGTTAACAAAAGTTGTAAATATAATGAGGGACGAAACTATTGATGAAAGTGTGAAGGCATGTGCCTCATTAATATTTGAGCCTTACTCATATATATACACTAATGTATTTTGACAGTAGATATAATTTTTTTTCCTGCATTTCAATGTGAAAAAGTTTAGGAAC
TARGET=65,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0248
PRIMER_LEFT_PENALTY=0.021195
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=AGAGTAACGCAGGGGAAACATA
PRIMER_RIGHT_SEQUENCE=TGGACATTGAATAGCACCTTTG
PRIMER_LEFT=32,22
PRIMER_RIGHT=321,22
PRIMER_LEFT_TM=60.021
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=290
PRIMER_PAIR_PENALTY_1=0.0489
PRIMER_LEFT_1_PENALTY=0.021195
PRIMER_RIGHT_1_PENALTY=0.027715
PRIMER_LEFT_1_SEQUENCE=AGAGTAACGCAGGGGAAACATA
PRIMER_RIGHT_1_SEQUENCE=ACATTGAATAGCACCTTTGCCT
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=318,22
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.0489
PRIMER_LEFT_2_PENALTY=0.021195
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=AGAGTAACGCAGGGGAAACATA
PRIMER_RIGHT_2_SEQUENCE=ATAGCACCTTTGCCTTCACAAT
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=311,22
PRIMER_LEFT_2_TM=60.021
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.0613
PRIMER_LEFT_3_PENALTY=0.021195
PRIMER_RIGHT_3_PENALTY=0.040146
PRIMER_LEFT_3_SEQUENCE=AGAGTAACGCAGGGGAAACATA
PRIMER_RIGHT_3_SEQUENCE=TTGCATCCTGGACATTGAATAG
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=329,22
PRIMER_LEFT_3_TM=60.021
PRIMER_RIGHT_3_TM=59.960
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.0613
PRIMER_LEFT_4_PENALTY=0.021195
PRIMER_RIGHT_4_PENALTY=0.040146
PRIMER_LEFT_4_SEQUENCE=AGAGTAACGCAGGGGAAACATA
PRIMER_RIGHT_4_SEQUENCE=CTTGCATCCTGGACATTGAATA
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=330,22
PRIMER_LEFT_4_TM=60.021
PRIMER_RIGHT_4_TM=59.960
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_04711 Ps_contig_mira-and-tgicl-ass_franssen_34392
SEQUENCE=TCGAAAATCCCNAATCGCGGTATTTGTTCCTCAAACACGCCGTCGTCGACGACGCAACCGGAACCCGGATCACACGAAGCTCAACAACTGCGAGCAAACAAACTTCCCCAATCTATGGTGCAACGGCCGCGACTCTCAACCGAGTTCGACCATCACTCGCAATCGCGAATTCAAGCGCTTCGTCTTCTGCACGTGTGTACCAGTGTTGGCTTTGCAAGAGCATTCTAGGGGTATGCTCAGAGTCACGTTCCGAAATTCGAGATGAAGATGAAAGTGACTCAATCGTTCACTCTTCATCTCTGCTTCACCTGGCCTGCACGAATTCAAGATGAAGACGAGAGGAGTGAGAACTCACCGTATCAATGTCGGCAATACGCTCTTCGTGGATTACTTTGTCATCTTCTCCTTCCGTAATCTTCCCTCCTCCTGCAAACGAATTCTGACACGGTTTCAACGTCGTCAACTACTTTACCGATAACACTTCTTCATCTCTCTGTTCCCTTTCCCTCTTATCTTACTTTAATCTCTGCTTCTTCTTTGCATATGCTCATGGTTATTGTGAATTGCAGCATGAAAAGTTGGCGGCAGAGTGATGACAGAAGATTAGAGACTCTTGGTATGAAGAAGATTCCCGCCGTAGAAGAAGAAGATTGGAGTGAAGAACAATGGAGATTGGAATGAAAGGGAAGAAACGGTTGTGAATTCCATTTCATAGTGAAGTCTGCCCCGTGAAAAATAAGGTTGATGCAGTTGGGAATCCGGCCGCCAAGCATATCCTGCAGCATACTGTCATGCTGGTGTATTGCAACAGCTATTTCCTTGCTGGCTTGCGTCGAGTGCATGAAGTTACACGCTTATTCAATTGGAGAATTCAAACTCAAATCGGTTAGCAGTTGAGGTTTGTGAGCTGGTTCAATTAACTTCAGTATGCACCGTTTCTTTTGGTTCAGTTTTGTGGAGTGTTGCTCAGAAGGGAATGAAATGGTATTGCTTGTGTCAATTTTCCTGCCAAGTACTGCTTCTGCTATCTACCATGGCCATGATTGAATTGTGTTGCGGAACGAGGACTGCACCTTGCTTGGATTGCATTGGATTTGTAATTATGTTAATCATTGTAAGCATGTGTAGGCTATAATTTGGACGTAGTTTGGAGCTTGGTTGAAGAATGCTCATGCTCATCCTTGTAGCTTTTCTTTTTAGGCAACGTTTGAATCGGATCAATGTAATGTAGGAACATGTAAACCGTGATTTGAAAATGAGTTGGAAATTTATGAAATTGCAAAGATTTATGTAGTTTGGAAATTCTTCTGANTGTTGATCTGGAATTTGAATTTAAG
TARGET=639,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0953
PRIMER_LEFT_PENALTY=0.040447
PRIMER_RIGHT_PENALTY=0.054900
PRIMER_LEFT_SEQUENCE=CTTGGTATGAAGAAGATTCCCG
PRIMER_RIGHT_SEQUENCE=CCGATTTGAGTTTGAATTCTCC
PRIMER_LEFT=612,22
PRIMER_RIGHT=885,22
PRIMER_LEFT_TM=59.960
PRIMER_RIGHT_TM=59.945
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.2771
PRIMER_LEFT_1_PENALTY=0.040447
PRIMER_RIGHT_1_PENALTY=0.236697
PRIMER_LEFT_1_SEQUENCE=CTTGGTATGAAGAAGATTCCCG
PRIMER_RIGHT_1_SEQUENCE=CCCAACTGCATCAACCTTATTT
PRIMER_LEFT_1=612,22
PRIMER_RIGHT_1=754,22
PRIMER_LEFT_1_TM=59.960
PRIMER_RIGHT_1_TM=60.237
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.3114
PRIMER_LEFT_2_PENALTY=0.074695
PRIMER_RIGHT_2_PENALTY=0.236697
PRIMER_LEFT_2_SEQUENCE=ATCTCTCTGTTCCCTTTCCCTC
PRIMER_RIGHT_2_SEQUENCE=CCCAACTGCATCAACCTTATTT
PRIMER_LEFT_2=487,22
PRIMER_RIGHT_2=754,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=60.237
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.4670
PRIMER_LEFT_3_PENALTY=0.230329
PRIMER_RIGHT_3_PENALTY=0.236697
PRIMER_LEFT_3_SEQUENCE=TCTTCTTTGCATATGCTCATGG
PRIMER_RIGHT_3_SEQUENCE=CCCAACTGCATCAACCTTATTT
PRIMER_LEFT_3=531,22
PRIMER_RIGHT_3=754,22
PRIMER_LEFT_3_TM=60.230
PRIMER_RIGHT_3_TM=60.237
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.4689
PRIMER_LEFT_4_PENALTY=0.232197
PRIMER_RIGHT_4_PENALTY=0.236697
PRIMER_LEFT_4_SEQUENCE=CTCTCTGTTCCCTTTCCCTCTT
PRIMER_RIGHT_4_SEQUENCE=CCCAACTGCATCAACCTTATTT
PRIMER_LEFT_4=489,22
PRIMER_RIGHT_4=754,22
PRIMER_LEFT_4_TM=60.232
PRIMER_RIGHT_4_TM=60.237
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_04712 Ps_contig_mira-and-tgicl-ass_franssen_34399
SEQUENCE=ACTAAATTGCGTGGATTGRGNNGGCGGAGTAGAAGATTGGATTAAACAACACGGAGGTTGTTAATGGAGTAGTTTGTGTGTGTAGTGTGTAGGTATGAATAAATGATCTGAGTTAATTAACTAGACAAAGTTAAAGGAAAAAGAAAAGAAAAGGAAAAAGCGAAGATGAGTCGGAGAGGAGATGAAGAATACGATTATCTGTTCAAAGTGGTACTGATCGGCGATTCCGGTGTCGGTAAATCAAATCTTCTTTCCCGATTCACTCGCAATGAATTTTGTCTCGAATCCAAATCCACCATCGGCGTCGAATTCGCCACACGCACACTTCAGGTTGAGGGAAGAACTGTGAAATCTCAGATATGGGATACTGCCGGACAGGAACGTTACAGGGCGATTACGAGTGCCTACTATCGCGGTGCACTCGGAGCTCTTCTCGTCTATGATGTCACCAAACCTACTACCTTTGACAATGTCACCAGATGGCTCAAGGAACTCAGGGATCATGCTGATGCCAACATTGTCATCATGTTGATCGGCAATAAAACCGATCTCAAGCATCTCCGTGCTGTTAACACAGAAGATGCTCAGAGTTATGCTGAGAGGGAAGGTCTTTCTTTCATCGAGACGTCTGCGCTTGAAGCCACCAATGTCGAGAAGGCTTTCCAGACGATTCTCGGGGAGATATACCGTATAATCAGCAAGAAGTCACTGTCGTCTTCCAATGATTCTGCTGCAGCTTCCAATATTAAAGAAGGTAAGACCATTACCATTGAAGGATCCGAAACCACCACTACAAACAAGCCCTGCTGTACATCGTAAACTTTAACTTCTTCAACCGGGACTCACTCGCGAGGGAGATATTATATTTTTATAGATACACTACAACTTTGTCTTTTCTATTTTCTTTATTTATTTTGTTTCTTATTGTTTTGCTCACTATCAGGAATCAGGCTCAACATTGCTTCTATTTGTTTTGTCGCCTTATCTATTTCTTTTTTCTAACTTTTGTTTTCTTAAA
TARGET=139,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04713 Ps_contig_mira-and-tgicl-ass_franssen_34402
SEQUENCE=ATTGGAAAAAAGTACCTTTAAATTTGGACAAAGTTACCAGACATGGGCTTACTAGAACTTCTATAGACATAATAATGCAAGTAATCTATTCACGGACACCCTCAGCTGCATGTGCTTGTTCCCAATATCAGTTTTAAACGCCAAATCATACAATTTTCAATCCAAATCTTCTTGCCTATTTCCCAATCCCCTTCCATCAGAGTCCTGAGCAGAGTTACCAGAACCAAAAGGATACCGCAAAGATATTCTAAAAGTCCTGTGGACAGGTCTATTTCCTCCTCCTCCACCTGCACCACCAGCACTGTTACCATCAACAACAGACCTCAACCCATCACCACCACCACCACCACCACTCCTATCCAGGTTCTCATAATCAGCATCATCAGTAGGCAATTCATATCGACAAACAGGGCAAGAGTTATGCAATTGAAGCCATGGAAGCAAGCAATCATCATGATACACATGCTTACAAGGCATTTGCTTCACTTGCAAACCCTTCTCAAACTCATCCTGACAAACAGCACACTGGTTCATCTCCGAGTTCATCAACTCATCATCAACAGTGACACTCGGAAGATTCTGAACAGCGTCTTTCGATGCCGGCGGAGTCCCATAACGGTTAGGATCATTCTCAGCAAGCTGCTGAATCAATTGCTCAAGACCAGGACCGAGGAAGTAATCCCCCATGTTAGACGGAACGCGAAACCCTGATTCGGATTCAGAAGGATGATTGATCTCGAACTGAATGTTTGCACCATCGGCATGAAGTCCATTAAGGTGATTCTGAAGGAAAGTGAACGGGTCGAAGTTATCGGTTTCCGTTTCGGTGGTTTCCGTCGTTTCGAGCCGGGTCCTGGNAA
TARGET=274,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0919
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=TGAGCAGAGTTACCAGAACCAA
PRIMER_RIGHT_SEQUENCE=GAGAACCTGGATAGGAGTGGTG
PRIMER_LEFT=205,22
PRIMER_RIGHT=368,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.0919
PRIMER_LEFT_1_PENALTY=0.086339
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=TGAGCAGAGTTACCAGAACCAA
PRIMER_RIGHT_1_SEQUENCE=TGAGAACCTGGATAGGAGTGGT
PRIMER_LEFT_1=205,22
PRIMER_RIGHT_1=369,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=0.1141
PRIMER_LEFT_2_PENALTY=0.086339
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=TGAGCAGAGTTACCAGAACCAA
PRIMER_RIGHT_2_SEQUENCE=AATTGCATAACTCTTGCCCTGT
PRIMER_LEFT_2=205,22
PRIMER_RIGHT_2=427,22
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.1141
PRIMER_LEFT_3_PENALTY=0.086339
PRIMER_RIGHT_3_PENALTY=0.027715
PRIMER_LEFT_3_SEQUENCE=TGAGCAGAGTTACCAGAACCAA
PRIMER_RIGHT_3_SEQUENCE=ATTGCATAACTCTTGCCCTGTT
PRIMER_LEFT_3=205,22
PRIMER_RIGHT_3=426,22
PRIMER_LEFT_3_TM=59.914
PRIMER_RIGHT_3_TM=60.028
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.2351
PRIMER_LEFT_4_PENALTY=0.229480
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=TACCAGAACCAAAAGGATACCG
PRIMER_RIGHT_4_SEQUENCE=GAGAACCTGGATAGGAGTGGTG
PRIMER_LEFT_4=215,22
PRIMER_RIGHT_4=368,22
PRIMER_LEFT_4_TM=60.229
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_04714 Ps_contig_mira-and-tgicl-ass_franssen_34402
SEQUENCE=ATTGGAAAAAAGTACCTTTAAATTTGGACAAAGTTACCAGACATGGGCTTACTAGAACTTCTATAGACATAATAATGCAAGTAATCTATTCACGGACACCCTCAGCTGCATGTGCTTGTTCCCAATATCAGTTTTAAACGCCAAATCATACAATTTTCAATCCAAATCTTCTTGCCTATTTCCCAATCCCCTTCCATCAGAGTCCTGAGCAGAGTTACCAGAACCAAAAGGATACCGCAAAGATATTCTAAAAGTCCTGTGGACAGGTCTATTTCCTCCTCCTCCACCTGCACCACCAGCACTGTTACCATCAACAACAGACCTCAACCCATCACCACCACCACCACCACCACTCCTATCCAGGTTCTCATAATCAGCATCATCAGTAGGCAATTCATATCGACAAACAGGGCAAGAGTTATGCAATTGAAGCCATGGAAGCAAGCAATCATCATGATACACATGCTTACAAGGCATTTGCTTCACTTGCAAACCCTTCTCAAACTCATCCTGACAAACAGCACACTGGTTCATCTCCGAGTTCATCAACTCATCATCAACAGTGACACTCGGAAGATTCTGAACAGCGTCTTTCGATGCCGGCGGAGTCCCATAACGGTTAGGATCATTCTCAGCAAGCTGCTGAATCAATTGCTCAAGACCAGGACCGAGGAAGTAATCCCCCATGTTAGACGGAACGCGAAACCCTGATTCGGATTCAGAAGGATGATTGATCTCGAACTGAATGTTTGCACCATCGGCATGAAGTCCATTAAGGTGATTCTGAAGGAAAGTGAACGGGTCGAAGTTATCGGTTTCCGTTTCGGTGGTTTCCGTCGTTTCGAGCCGGGTCCTGGNAA
TARGET=333,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1141
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=TGAGCAGAGTTACCAGAACCAA
PRIMER_RIGHT_SEQUENCE=AATTGCATAACTCTTGCCCTGT
PRIMER_LEFT=205,22
PRIMER_RIGHT=427,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=223
PRIMER_PAIR_PENALTY_1=0.1141
PRIMER_LEFT_1_PENALTY=0.086339
PRIMER_RIGHT_1_PENALTY=0.027715
PRIMER_LEFT_1_SEQUENCE=TGAGCAGAGTTACCAGAACCAA
PRIMER_RIGHT_1_SEQUENCE=ATTGCATAACTCTTGCCCTGTT
PRIMER_LEFT_1=205,22
PRIMER_RIGHT_1=426,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.2530
PRIMER_LEFT_2_PENALTY=0.229480
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=TACCAGAACCAAAAGGATACCG
PRIMER_RIGHT_2_SEQUENCE=GGATGAGTTTGAGAAGGGTTTG
PRIMER_LEFT_2=215,22
PRIMER_RIGHT_2=510,22
PRIMER_LEFT_2_TM=60.229
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=0.2572
PRIMER_LEFT_3_PENALTY=0.229480
PRIMER_RIGHT_3_PENALTY=0.027715
PRIMER_LEFT_3_SEQUENCE=TACCAGAACCAAAAGGATACCG
PRIMER_RIGHT_3_SEQUENCE=ATTGCATAACTCTTGCCCTGTT
PRIMER_LEFT_3=215,22
PRIMER_RIGHT_3=426,22
PRIMER_LEFT_3_TM=60.229
PRIMER_RIGHT_3_TM=60.028
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.2572
PRIMER_LEFT_4_PENALTY=0.229480
PRIMER_RIGHT_4_PENALTY=0.027715
PRIMER_LEFT_4_SEQUENCE=TACCAGAACCAAAAGGATACCG
PRIMER_RIGHT_4_SEQUENCE=AATTGCATAACTCTTGCCCTGT
PRIMER_LEFT_4=215,22
PRIMER_RIGHT_4=427,22
PRIMER_LEFT_4_TM=60.229
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_04715 Ps_contig_mira-and-tgicl-ass_franssen_34405
SEQUENCE=GGTTTTTTCTTAAACTTTGTAAAAGACTTATGAAAACAAACTGAAACAACTCATGAATACATAACTATTTTTATTAAAGGCTTAACGAATANTCATGATATAAGATATGCATAAATAATTCTTCCAAACTTACTAAGCACAATTGTTGTTGTCGTGCAACTGAGAAAACATGGTTTTGCACTATTGTTTGATACACAAATTATTCAGTAAAATAATATTCCCTGCCTAAATAAAGCATGTGAGAGAGGTATAATAAATGTTGGAAGCAAGCAGTATTTATATTAGCATTGGTCAAAACTCAGCAAAAAAATAGCACCAGACTCCTTATTGCATGACAATACCTATTGGGTGTTATGGAATCAATGGGCAAATAATGCCTTGTACTTTTCCAGGAACCTTGATTCAGAACGAAAGACCAAGAACCATTGCAATGCCAACAACGGCGCATGCCACCATTCTGCAATAGACATTGTGTTAAGAACTACCTTGGTACTATGCCACTGAGATCTAACCAGCTGGGAACACCAATATCATGCCAGACTGCAATTTATCCAACTCACAACTTATTAAATACTATTATTTTTTGTATACACTCATTTGTGTCTCCATCATGTACTGCTGTCGTACATTGAATTCTCTGTTGAGTGTGAAATGTTAAGATTAAATGCAGACCGCAACTACAGAAAAATACAAGATCAACTACTCCACTTGTCCACTTAATTCTGCTTCCTCATCCTCGGCATCCATTTCTTGTTCCACGCCTTTGACCTCAGGTACATAATGCATCAGCATATTCTCAATGCCTGATTTCAGAGTCACCGAAGAACTTGGGCAGCCACTACACGCTCCTTGCATTTTAAGTTTGACTATCCCGGTATCTGGGTCAAAACCACAATATACAATGTCCCCACCATCATCTTGTACAGCTGGTCGAATACGAGTCTCTAAGAGTTCCTTGATCATCGCAACTGTTTCAGAATCATCATCGTGAATTGCGGTGTCTTTGGATGCAGCAGCTTGAGAATCTAGAAAGAGAGGTTCACCGGAACAGTAGAAATCCATAATAGAAGCAAAGATTTCCGGCTTAAGAAATTCCCAGGAAGCATCTTCGGATTTGGTAACGGTAACAAAATCAGATCCAAAGAAAACACGAGTAATTCCGTCAATAGCGAAGAGTGATTTGGCGAGAGGAGAATTCAAGGCGGAACGAGCGTTAGGGAAATCTGCGCTTCCGATATCCATAACAGGCTTACCGGGATGAAACATGAGAGATTCTGGATTTGGAGTGGACTGCGTTTGAATAAACATACTCCTCTTTTGTCCGGTGGTGGAAAACGGAGATGGAAATGGAGAGGTGGAGGAAGAGGAAGAGAAAGCATTGTTGTGGCGGTTGCGATGTTGAAACGACGGCGTTGCGGCGAGGTAGCGTGGACGAATAGTGATTAGGTTATTATTATTATTGGTTTCTGGTTTGAGAGAATTGAAGAGTTTTGATTGTAACCGTCTTCCCAATCGGAAAACACTAGTTCTCGCCATCGTTCTTCACGATTCGGGGTTTTCTGTTATCGTGTTCTGCTACAAAACCTCACGCTTCCTTCGTCAACCTCGAACGACGTCNTTTTAGTT
TARGET=1448,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1678
PRIMER_LEFT_PENALTY=0.039178
PRIMER_RIGHT_PENALTY=0.128591
PRIMER_LEFT_SEQUENCE=GTGGACTGCGTTTGAATAAACA
PRIMER_RIGHT_SEQUENCE=GAACTAGTGTTTTCCGATTGGG
PRIMER_LEFT=1285,22
PRIMER_RIGHT=1528,22
PRIMER_LEFT_TM=60.039
PRIMER_RIGHT_TM=59.871
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.2501
PRIMER_LEFT_1_PENALTY=0.039178
PRIMER_RIGHT_1_PENALTY=0.210886
PRIMER_LEFT_1_SEQUENCE=GTGGACTGCGTTTGAATAAACA
PRIMER_RIGHT_1_SEQUENCE=GCGAGAACTAGTGTTTTCCGAT
PRIMER_LEFT_1=1285,22
PRIMER_RIGHT_1=1532,22
PRIMER_LEFT_1_TM=60.039
PRIMER_RIGHT_1_TM=59.789
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.2560
PRIMER_LEFT_2_PENALTY=0.039178
PRIMER_RIGHT_2_PENALTY=0.216800
PRIMER_LEFT_2_SEQUENCE=GTGGACTGCGTTTGAATAAACA
PRIMER_RIGHT_2_SEQUENCE=GGGAAGACGGTTACAATCAAAA
PRIMER_LEFT_2=1285,22
PRIMER_RIGHT_2=1509,22
PRIMER_LEFT_2_TM=60.039
PRIMER_RIGHT_2_TM=60.217
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=0.2925
PRIMER_LEFT_3_PENALTY=0.039178
PRIMER_RIGHT_3_PENALTY=0.253332
PRIMER_LEFT_3_SEQUENCE=GTGGACTGCGTTTGAATAAACA
PRIMER_RIGHT_3_SEQUENCE=GAACACGATAACAGAAAACCCC
PRIMER_LEFT_3=1285,22
PRIMER_RIGHT_3=1572,22
PRIMER_LEFT_3_TM=60.039
PRIMER_RIGHT_3_TM=59.747
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=10.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.2998
PRIMER_LEFT_4_PENALTY=0.171245
PRIMER_RIGHT_4_PENALTY=0.128591
PRIMER_LEFT_4_SEQUENCE=TTCCGATATCCATAACAGGCTT
PRIMER_RIGHT_4_SEQUENCE=GAACTAGTGTTTTCCGATTGGG
PRIMER_LEFT_4=1229,22
PRIMER_RIGHT_4=1528,22
PRIMER_LEFT_4_TM=59.829
PRIMER_RIGHT_4_TM=59.871
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=300
=
PRIMER_SEQUENCE_ID=SSR_04716 Ps_contig_mira-and-tgicl-ass_franssen_34411
SEQUENCE=TCAACGCAGAGTACGCGGGGACACCAGCAACGTTGCCCAATATCAAAACCTTGAAACTCTTCATTTTCCATTCTTGTATCCTTAACTGATTATACAATATTGCATTATGGCAACTAATTCTCTTCATTCAATTCAACACCATTCCCTCATCAAACGCCAATACTACTACTACCCTTCTGAAACAGCTCCTCCATCCACACTTTTCCTCAAACCAACCAGCACCAGCATAACCCTCTCCCACTCTTCAACTTCCAGCACTTTTACCACATTCTCACTCTCTCAAACAACATCCTCCACACCATCATCAACCTCTCAATCCTCCTCCTTTGAACTCCTCCAGCAGCTTCTCTCAGCCAAAAACTTCCGAGAAGCCGACGACGAGACAAGGCGGCTTCTCATCGTGTTGGCAGGCGAAGCGGCTCAGAAGCGGGGATATGTTTTCTTCTCAGAGGTTCAGTTCATCTCGGAAACAGACCTCCAAACAATTGACAAGCTGTGGAGGGAGCATAGTGACAACAAGTTTGGATACAGTGTTCAGAAGAAACTATTTGGTAAAGCTAAAAAGGACTTCACCAACTTCTTCATTAAGGTTGGTTGGATGAAGAAACTTGACACTGAGATGGAGCAATATAACTATAGGTCTTTTCCTACAGAGTTTACATGGGAGCTCAATGATGATACACCACAGGGTCATTTACCTTTGACTAATGCACTCAGAGGAACACAGTTGCTGAGTAATATTTTTAGCCATCCAGCTTTTGATGCAGTTCAAGATGAAGTTGAAGTTTCATTTGGTAACACTGGGGGATTCAAAGGGTTAAAAGATTCATCAAAGACATCAGCTCAGAATATCCTCAAAACAGACTATAGTTTCTGATAGAGTTCAGGACAAAGTTTACAATAATAAAGCTTGATGAATGCATAGAGAAGAATGTAACTTAATTTGTGTTTTAATTTTTAAGTATATACGTAGTAATTATTGATTCATCATCATCAAGAGATGAAGGTAGTTGCTTGAAGTGTATTTTTTTACTTTAATAAGGATAACTGCATTCTACAAACTCTTTAAACTAAAGGTTCATATGTTAGCTGTCATTAATTTTGTGACT
TARGET=161,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1349
PRIMER_LEFT_PENALTY=0.060196
PRIMER_RIGHT_PENALTY=0.074695
PRIMER_LEFT_SEQUENCE=AATTCAACACCATTCCCTCATC
PRIMER_RIGHT_SEQUENCE=GAGGAGTTCAAAGGAGGAGGAT
PRIMER_LEFT=129,22
PRIMER_RIGHT=336,22
PRIMER_LEFT_TM=60.060
PRIMER_RIGHT_TM=60.075
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.2754
PRIMER_LEFT_1_PENALTY=0.060196
PRIMER_RIGHT_1_PENALTY=0.215215
PRIMER_LEFT_1_SEQUENCE=AATTCAACACCATTCCCTCATC
PRIMER_RIGHT_1_SEQUENCE=TGGTAAAAGTGCTGGAAGTTGA
PRIMER_LEFT_1=129,22
PRIMER_RIGHT_1=265,22
PRIMER_LEFT_1_TM=60.060
PRIMER_RIGHT_1_TM=59.785
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=0.3696
PRIMER_LEFT_2_PENALTY=0.060196
PRIMER_RIGHT_2_PENALTY=0.309418
PRIMER_LEFT_2_SEQUENCE=AATTCAACACCATTCCCTCATC
PRIMER_RIGHT_2_SEQUENCE=GTTCAAAGGAGGAGGATTGAGA
PRIMER_LEFT_2=129,22
PRIMER_RIGHT_2=331,22
PRIMER_LEFT_2_TM=60.060
PRIMER_RIGHT_2_TM=59.691
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.5891
PRIMER_LEFT_3_PENALTY=0.576589
PRIMER_RIGHT_3_PENALTY=0.012470
PRIMER_LEFT_3_SEQUENCE=GTTGCCCAATATCAAAACCTTG
PRIMER_RIGHT_3_SEQUENCE=GTGTGGAGGATGTTGTTTGAGA
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=298,22
PRIMER_LEFT_3_TM=60.577
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.6505
PRIMER_LEFT_4_PENALTY=0.576589
PRIMER_RIGHT_4_PENALTY=0.073866
PRIMER_LEFT_4_SEQUENCE=GTTGCCCAATATCAAAACCTTG
PRIMER_RIGHT_4_SEQUENCE=GAGGAGGATTGAGAGGTTGATG
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=323,22
PRIMER_LEFT_4_TM=60.577
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_04717 Ps_contig_mira-and-tgicl-ass_franssen_34411
SEQUENCE=TCAACGCAGAGTACGCGGGGACACCAGCAACGTTGCCCAATATCAAAACCTTGAAACTCTTCATTTTCCATTCTTGTATCCTTAACTGATTATACAATATTGCATTATGGCAACTAATTCTCTTCATTCAATTCAACACCATTCCCTCATCAAACGCCAATACTACTACTACCCTTCTGAAACAGCTCCTCCATCCACACTTTTCCTCAAACCAACCAGCACCAGCATAACCCTCTCCCACTCTTCAACTTCCAGCACTTTTACCACATTCTCACTCTCTCAAACAACATCCTCCACACCATCATCAACCTCTCAATCCTCCTCCTTTGAACTCCTCCAGCAGCTTCTCTCAGCCAAAAACTTCCGAGAAGCCGACGACGAGACAAGGCGGCTTCTCATCGTGTTGGCAGGCGAAGCGGCTCAGAAGCGGGGATATGTTTTCTTCTCAGAGGTTCAGTTCATCTCGGAAACAGACCTCCAAACAATTGACAAGCTGTGGAGGGAGCATAGTGACAACAAGTTTGGATACAGTGTTCAGAAGAAACTATTTGGTAAAGCTAAAAAGGACTTCACCAACTTCTTCATTAAGGTTGGTTGGATGAAGAAACTTGACACTGAGATGGAGCAATATAACTATAGGTCTTTTCCTACAGAGTTTACATGGGAGCTCAATGATGATACACCACAGGGTCATTTACCTTTGACTAATGCACTCAGAGGAACACAGTTGCTGAGTAATATTTTTAGCCATCCAGCTTTTGATGCAGTTCAAGATGAAGTTGAAGTTTCATTTGGTAACACTGGGGGATTCAAAGGGTTAAAAGATTCATCAAAGACATCAGCTCAGAATATCCTCAAAACAGACTATAGTTTCTGATAGAGTTCAGGACAAAGTTTACAATAATAAAGCTTGATGAATGCATAGAGAAGAATGTAACTTAATTTGTGTTTTAATTTTTAAGTATATACGTAGTAATTATTGATTCATCATCATCAAGAGATGAAGGTAGTTGCTTGAAGTGTATTTTTTTACTTTAATAAGGATAACTGCATTCTACAAACTCTTTAAACTAAAGGTTCATATGTTAGCTGTCATTAATTTTGTGACT
TARGET=985,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.4206
PRIMER_LEFT_PENALTY=0.494198
PRIMER_RIGHT_PENALTY=2.926422
PRIMER_LEFT_SEQUENCE=GTTTCATTTGGTAACACTGGGG
PRIMER_RIGHT_SEQUENCE=CACTTCAAGCAACTACCTTCATC
PRIMER_LEFT=784,22
PRIMER_RIGHT=1021,23
PRIMER_LEFT_TM=60.494
PRIMER_RIGHT_TM=58.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=3.4273
PRIMER_LEFT_1_PENALTY=0.500838
PRIMER_RIGHT_1_PENALTY=2.926422
PRIMER_LEFT_1_SEQUENCE=GGGGATTCAAAGGGTTAAAAGA
PRIMER_RIGHT_1_SEQUENCE=CACTTCAAGCAACTACCTTCATC
PRIMER_LEFT_1=803,22
PRIMER_RIGHT_1=1021,23
PRIMER_LEFT_1_TM=60.501
PRIMER_RIGHT_1_TM=58.074
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=3.4695
PRIMER_LEFT_2_PENALTY=0.494198
PRIMER_RIGHT_2_PENALTY=2.975341
PRIMER_LEFT_2_SEQUENCE=GTTTCATTTGGTAACACTGGGG
PRIMER_RIGHT_2_SEQUENCE=CACTTCAAGCAACTACCTTCATCT
PRIMER_LEFT_2=784,22
PRIMER_RIGHT_2=1021,24
PRIMER_LEFT_2_TM=60.494
PRIMER_RIGHT_2_TM=59.025
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=3.4762
PRIMER_LEFT_3_PENALTY=0.500838
PRIMER_RIGHT_3_PENALTY=2.975341
PRIMER_LEFT_3_SEQUENCE=GGGGATTCAAAGGGTTAAAAGA
PRIMER_RIGHT_3_SEQUENCE=CACTTCAAGCAACTACCTTCATCT
PRIMER_LEFT_3=803,22
PRIMER_RIGHT_3=1021,24
PRIMER_LEFT_3_TM=60.501
PRIMER_RIGHT_3_TM=59.025
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=219
PRIMER_PAIR_PENALTY_4=3.5979
PRIMER_LEFT_4_PENALTY=0.494198
PRIMER_RIGHT_4_PENALTY=3.103672
PRIMER_LEFT_4_SEQUENCE=GTTTCATTTGGTAACACTGGGG
PRIMER_RIGHT_4_SEQUENCE=ACACTTCAAGCAACTACCTTCATC
PRIMER_LEFT_4=784,22
PRIMER_RIGHT_4=1022,24
PRIMER_LEFT_4_TM=60.494
PRIMER_RIGHT_4_TM=58.896
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_04718 Ps_contig_mira-and-tgicl-ass_franssen_34414
SEQUENCE=GCATCTCTATAANAATCTCCTCAATTTTACTCCAAAAAAACAACTTAAAACTATCGCAACTCAAACCCAAACACACGCTCTCTATGTATTTACCAATCATTTGATCGCCGGAACACATCTCCGGCAATCCACCTCCGATCATTGACCACCACTCCAACCCTCGGTGACCATCCAATCACCTCAGATCCACCGTCGTTTAAGCTGTGATGCGAAAATCGTGGCAGAGGAACACTCTACGACGTCGTAGCTTTCATCTTCAACAATCACTTCTGAAATTGTTAGCTTAGGGTTAGGTTAGGGTTTGCGAAAAAAATGTTCAAGAGAATGATGAAGGGAGGGCAAAAGAAGCCCTCCAAATCGGATTCTAACGATGGATACGGTCCTCCGGCGAACGACGTCAATCAATCAGCGGTTGTGGTCCTACCTCCGCCGTCTGGCAAAATCGAACCGCTTCCGTTGTTCCGTGATGTACCGGTTTCTGAGAGGCAGAATTTGTTTCTCCGGAAGCTTCAGATTTGTTGCCACACGTTGGATTTCACCGACACGATGAAATCAGTTCGGGAGAAGGAAATTAAGAGACAGACTTTGATGGAATTGGTTGAATTTATTCAATCTAGTTCGAGTAAGATAACGGAGATTTGTCAAGAAGAGATGATTAAAATGGTGTCGGTGAATATTTTCCGGTGTCTCCCACCGGCATTCCACGAGAACACGGGACAGGATGCTACCGATCCGGAAGAGGAGGAGCCTTGTTTGGAACCGGCGTGGCCTCATTTGCAGCTCGTTTATGAGCTTCTCCTTAGATACGTGGTTTCGTCTGATACTGATACTAAGGTAGCTAAACGTTACATTGATCATTCATTTGTGCTTAAATTGCTTGATTTGTTTGATTCTGAGGATCCTAGAGAACGTGAGTATGTGAAAACTATCTTGCATCGTGTGTATGGAAAATTTATGGTTCATAGACCCTATATTAGGAAGGCTATTAACAACATATTTTACCGGTTTATATATGAGACTGAGAGACATAGTGGGATTATGGAGCTTCTTGAGATTCTTGGGAGCATCATTAATGGGTTTGCTTTGCCCATGAAGGAGGAGCATAAATTGTTTCTTGTGAGGGCATTGTTGCCTTTGCATAAACCTAAACCCGTAGCTGTGTACCATCAGCAGTTGTCGTACTGCATTTCTCAGTTTGTTGAGAAGGATTTTAAGCTAGCGGATACTGTTATTAGGGGTTTGTTGAAATACTGGCCCGTCACCAATTGCCAGAAGGAAGTTCTATTTCTCGGGGAACTGGAGGAGGTGCTGGAGGCTACACAAGCCGCAGAATTCCAACGCTGCATGGTTCTTCTTTTTAGACAGATTGCACGCTGTCTCAATAGTCCGCATTTTCAGGTTGCAGAACGAGCCCTCTTTCTGTGGAATAATGAGCACATTGTCGGTTTAATAGCCCAAAACAGGACTGTAGTATTACCCATTATATTCGAAGCATTGGAGAAAAATGTCCAGAGTCATTGGAACCAGGCCGTTAACGGGTTGACGGTGAATGTTAGAAAAATGTTCCTCGAAATGGATGCTGATTTGTTTGAAGAGTGCCAGAGGAAGTATTTGGAGAAAGAGGCTAAAGCCAAAGAGGTGGAAGAGCTGCGGGAATTGAATTGGAAGAGATTGGCAGATGCCGCCGCACTGAATGGGCCGGATATGGTCACAGCTTAGCCAATTTGAAATTACTTTACTGCCCCAACTGGGATTGTTCATTGGATAGGTGGAGGATTATATTTTATATACTCTCCTTGCCTTTTTGGTGCCACTATTGATTGGTTTCTTTCTTTCTCCCCTGTAATTTATTTTTGCTCTCCATTTTCTCAGTTGGATAATCAGAAAGTTAAAGAAAGCAGTGATTAATGTAAGATAAAAATGTATTGGACGTTATTAGCAATGTCTGCAGATTAAGAATAGTTTTRTAAAANTTCTTGTTATTTTTTCCGTACAGATATGGTAATTTGTTAACTTTACCATCTATGCTTTATTTTTTTACT
TARGET=1825,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04719 Ps_contig_mira-and-tgicl-ass_franssen_34418
SEQUENCE=ACAATTAATTTAAACCAAAAAATTCAGTGTCACAAGATGCAAAAAGAGCTCATATAAATAAATTCATAATACATACATCRAGGGTTGAAAAAGTTAGAGAAAAGTTGAAACACTCATGGATCCAAATGATAATAGCTCTTGTGAATCTTCTTTTTGTAAAGATGAAAAAGTGGAAGCAATGAACCTTCTTGAAGAGTGTTGGTTCTTTGATAACTTGTTGAATATAAAACCAAAGATGCTAAGGTCTCACTCTGATCCTTACCCTTCCACTAGGCTTATAGACTCAGTATTTTTTGGTCAAGGACAAAAGTGCTTTTGTTTGTTCAAAGAAGATTCAAAGAACACCATCTATGCCACCAATTAGAGTGAAAAAAGAAGAAGATGAAGATGATGATGATAAGTTGGTGCATCAACCATTAGATCCTAGTAAACAACATCATTGTGCAAAGATGAAAGGACTTCATCATAGAAGGAAAAGCAAATTGTTGAGAACACCATCTTTGCCTCCATCAATAGGGAGAGAGGAAAAGTCTCAAGAAACTTATCCTAGAAATGGAAGATCCAAAAAACAACCATCAACACCTACCAATATTGACAAGTTGCCTCCAAGGTTAACCTCTAAGAGTTGTAGCATACCAAGATGTAGAGCATCCAAAAGCATTGAAGTTGAAAGCTTAAACAAAGAGGGTATTATGGAAATGAGGAGAAAGGTTCTAAATCAAAAAACAATAAGGAGAAGCTTAAGTGATCTTGAGTATGAAGAAGTTCAAGGGTTCAAGGATTTAGGATTCTCATTTGAGAAAGAAGCATTAAGTCCAAGTTTAGCTAACATAATTCCTGGTTTGCAAGAGAAAAATAGAGATGAAAGTGAGGAAGATAAAGCAGCAAGAGGACCTTATTTATCAGAGGCATGGTTAGTGCAAAGTCGTGCAACTCCTCCTGTTCCAAATTGGGGGGATAATATGTCTGCAGATGATATGAAAAAGCATATCAAGTTTTGGGCTAGAGCTGTGGCATCAACTAAATGTTGAATATGTATGATTATAATTCTTAAAATGATACTTCACATATCAATTTTTTGTAGCAATTGCTTAAGAACATCCAAAATAATAATCATAGTAATCATNATTTTCYATACTTT
TARGET=387,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04720 Ps_contig_mira-and-tgicl-ass_franssen_34421
SEQUENCE=ATCCAACCAGTTTAGCGGTTCACGGAACAAGAACGACGTCACTCTTCCATCTTATACACAGTAAAAGAAAAATACATATATATATTATTGGCNGTCGGCGGGTGGGGTAACGTTGCCGCTGGCACGCTGCAGCTTCACATTCACCTACTTCACCTACTTGTATAATTCAAGTTCAACTCTGACCACAAGATCAACCTAATCCCAAATCCCCAGTAATGTCTTTGTTGGCGTCGGAGATCGCTCGGAAACTGAATCTGCTTCCTCATCCCGAAGGTGGCTTTTACGCAGAAACATTCCGAGATCACTCCGTTCATCTCTCCACCTCTCACCTCCCTCCCGAATACAAAGTTGATCGTCCCGTTTCAACTTGCATATATTTTCTGCTTCCTTCTGGTAACATGTCTCGCCTTCATCGTATCCCTTGTTCCGAAACTTGGCATCATTACATCGGAGAACCCATCACCGTGGTGGAATTGAATGAGACAGATTCAAGTGTTAAGTTTACATGTGTTGGATCTGATCTAGCTAAGGATCAGACACCGCAATACACGGTTCCTCCCAATGTCTGGTTTGGTTCTTTTCCGACCAGTGATTTCACTGTCTCTGCGGATGGGTCATTTTTGAAGGGTCCAGAAAGGGATGCTGAGAGTCACTACTCACTTGTGGGATGCACTTGTGCACCTGGTTTTCAGTTCCAGGATTTTGAACTTGCTAAACGTTCTTACCTCGTTTCACGTTTTCCTCAATTCGAGACTCTCATCAATACTCTCACGTTTCCTGAGTAATTGATTGCATGTAGTTACTCAGTTTATAGTACCTTTCCAGAATCGAATAATTGTCCATGTTTACACTTTTTAATGCTCCTTTGATGGAAGAAAGTAAAACTGTTATATTGTAATTTTTATTACATGCTAAGAATTTCGAAACCTTGCAGTACTTGTGTGTATTATACAAAAATAAACATGTAACATGAATGAGTTATTACTTCTGTATTGGTTGCCTTTTGGTTTACTAGAGAAGAGAATAGTATATTTCCTAATAAAAAAATCTTACTAAGGATGA
TARGET=76,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2653
PRIMER_LEFT_PENALTY=0.174518
PRIMER_RIGHT_PENALTY=0.090770
PRIMER_LEFT_SEQUENCE=AACGACGTCACTCTTCCATCTT
PRIMER_RIGHT_SEQUENCE=CATTACTGGGGATTTGGGATTA
PRIMER_LEFT=31,22
PRIMER_RIGHT=217,22
PRIMER_LEFT_TM=60.175
PRIMER_RIGHT_TM=59.909
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.4560
PRIMER_LEFT_1_PENALTY=0.174518
PRIMER_RIGHT_1_PENALTY=0.281487
PRIMER_LEFT_1_SEQUENCE=AACGACGTCACTCTTCCATCTT
PRIMER_RIGHT_1_SEQUENCE=GGATGAGGAAGCAGATTCAGTT
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=267,22
PRIMER_LEFT_1_TM=60.175
PRIMER_RIGHT_1_TM=59.719
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=0.5932
PRIMER_LEFT_2_PENALTY=0.174518
PRIMER_RIGHT_2_PENALTY=0.418705
PRIMER_LEFT_2_SEQUENCE=AACGACGTCACTCTTCCATCTT
PRIMER_RIGHT_2_SEQUENCE=GGAGTGATCTCGGAATGTTTCT
PRIMER_LEFT_2=31,22
PRIMER_RIGHT_2=307,22
PRIMER_LEFT_2_TM=60.175
PRIMER_RIGHT_2_TM=59.581
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.7020
PRIMER_LEFT_3_PENALTY=0.174518
PRIMER_RIGHT_3_PENALTY=0.527457
PRIMER_LEFT_3_SEQUENCE=AACGACGTCACTCTTCCATCTT
PRIMER_RIGHT_3_SEQUENCE=AATGTTTCTGCGTAAAAGCCAC
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=60.527
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=0.7811
PRIMER_LEFT_4_PENALTY=0.690327
PRIMER_RIGHT_4_PENALTY=0.090770
PRIMER_LEFT_4_SEQUENCE=GAACGACGTCACTCTTCCATCT
PRIMER_RIGHT_4_SEQUENCE=CATTACTGGGGATTTGGGATTA
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=60.690
PRIMER_RIGHT_4_TM=59.909
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_04721 Ps_contig_mira-and-tgicl-ass_franssen_34423
SEQUENCE=AAATTTYAAAATACACAACTTGTATTAATCTTGATAACCCACCAAAATTTAAAAATAGCTTCTATACGAAATTTACAAAGGGAACTCTCAAATACATAGACAAATACAAGAATTTAAATCTGAAATAGAAACTGAAAATTAAGTCTTGAACAATTCCACTCAGCAGCTTCCATTCTATTGCACCTAGAATAAAAAAAATATATAATGCAGCAAATAGAGACTAGAGTGACTTCTCCATTGTTAACTTTTCCTTTTGATTTTTTCTTTTCCATTTCCTAGCACATAATTTACTTCGTCTTCCTCCGCATACCCATCCGCGATTTGGCATATTCTAAACGCATTCCTTCGCCAGCCGCTGAGGAATAAAGAATGGTGCCTTGCAGACTGTTTAAGGCGGCGGTTGCAGTCTCAACATCCTTGAAATCTACAAATGAAACAGGAGCCCCATATGTGCTTTGCATCTTCAGTTTCAGAAATCCAGGGAACCTGCAAAATACTTGATTTAGCTCTTGTTCATTACAAGTTGGCCCCAGATTAGCTACAAATAAAGTCGCGCATGGGATTGTATTTGTTGGAATGTAAGGAGTTGAACACTTTTTCAGATGTGGAAATAGACTGTCATGAAGAGCAAACCCGTCAGCATTTCCATGACAGAATCAGGAGTTGGCCATGAAGGTGCTGATCCTCGAGCTTTCTTATCTGAGCCAGCTCTCTCATCATCTATCCTTGCACGTTTAGCTCTAGAGTTCGATTTAGCCAGATCAATATATAGAGTGGAACCCTTTTCAAGATCAAAGACCATCCCATTAAGAGCATGCAATGCCATGATTGCAGATTGCTGATTCGAAAAAACAGCAAAAGCAAACGCCTGCGATGAATTGTTGGGACTTCTAAGATGCGATGACTCGTAACCGGGAAATTCTCGGAAGAGATTGTAGATTTCGCGGGGCTTAACATCTTCGGGAAGACCAGCGATGAAGAGAGTGCGAACATCGTTGGATGAAGATAGAGGAGGAACGTAAGAACCATAGACGGGTTGTTGCGGGTGGTACTGTGGCGGAAGGTGGTGGTGGTGCGGCGGCGGCGGTAGTGGAGCACCAGGGGGCGGTGGTATCACCGCCATGGGTTGANGAGGAGGCGGCGGCGGTGGATAATCNGATAAGTTGGNGATTCGGGTCGG
TARGET=1063,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04722 Ps_contig_mira-and-tgicl-ass_franssen_34423
SEQUENCE=AAATTTYAAAATACACAACTTGTATTAATCTTGATAACCCACCAAAATTTAAAAATAGCTTCTATACGAAATTTACAAAGGGAACTCTCAAATACATAGACAAATACAAGAATTTAAATCTGAAATAGAAACTGAAAATTAAGTCTTGAACAATTCCACTCAGCAGCTTCCATTCTATTGCACCTAGAATAAAAAAAATATATAATGCAGCAAATAGAGACTAGAGTGACTTCTCCATTGTTAACTTTTCCTTTTGATTTTTTCTTTTCCATTTCCTAGCACATAATTTACTTCGTCTTCCTCCGCATACCCATCCGCGATTTGGCATATTCTAAACGCATTCCTTCGCCAGCCGCTGAGGAATAAAGAATGGTGCCTTGCAGACTGTTTAAGGCGGCGGTTGCAGTCTCAACATCCTTGAAATCTACAAATGAAACAGGAGCCCCATATGTGCTTTGCATCTTCAGTTTCAGAAATCCAGGGAACCTGCAAAATACTTGATTTAGCTCTTGTTCATTACAAGTTGGCCCCAGATTAGCTACAAATAAAGTCGCGCATGGGATTGTATTTGTTGGAATGTAAGGAGTTGAACACTTTTTCAGATGTGGAAATAGACTGTCATGAAGAGCAAACCCGTCAGCATTTCCATGACAGAATCAGGAGTTGGCCATGAAGGTGCTGATCCTCGAGCTTTCTTATCTGAGCCAGCTCTCTCATCATCTATCCTTGCACGTTTAGCTCTAGAGTTCGATTTAGCCAGATCAATATATAGAGTGGAACCCTTTTCAAGATCAAAGACCATCCCATTAAGAGCATGCAATGCCATGATTGCAGATTGCTGATTCGAAAAAACAGCAAAAGCAAACGCCTGCGATGAATTGTTGGGACTTCTAAGATGCGATGACTCGTAACCGGGAAATTCTCGGAAGAGATTGTAGATTTCGCGGGGCTTAACATCTTCGGGAAGACCAGCGATGAAGAGAGTGCGAACATCGTTGGATGAAGATAGAGGAGGAACGTAAGAACCATAGACGGGTTGTTGCGGGTGGTACTGTGGCGGAAGGTGGTGGTGGTGCGGCGGCGGCGGTAGTGGAGCACCAGGGGGCGGTGGTATCACCGCCATGGGTTGANGAGGAGGCGGCGGCGGTGGATAATCNGATAAGTTGGNGATTCGGGTCGG
TARGET=1075,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04723 Ps_contig_mira-and-tgicl-ass_franssen_34424
SEQUENCE=ATAGACACGTGGCTGATTCTGTAGAATGAAGAAAAAATAAGGCACTGTTTGGATGCCACAGAAATAATCCATTTGCTGCGAAACTCAAAAGGAGGTAACTGAAATGGGGGACCTTTACGCTTTGGACTTCGATGGAGTCATCTGTGATAGCTGCGGTGAAAGCTCTCTCTCTGCTCTCAAGGCTGCTAAAGTGAGATGGCCTGGCTTGTTTGATGGTGTGGATTCAACCACTGAAGATTGGATTGTTGACCAAATGCATATTGTGCGACCAGTAGTGGAAACTGGATATGAAAACCTTTTACTTGTGAGATTGTTGCTAGAGACCAGAACACCTTCCATCAGGAAATCTTCGGTTGCAGAAGGGCTTGCAGTTGAGGGTATATTGGAGAATTGGTCCAAGTTGAAGCCTATTATTATGGAAGAATGGGGTGAGGATAGGGAGGCTTTGGTAGATCATTTTGGAAAGGTCAGAGATGAATGGTTGGAGCAGGATTTTGCTACTTGGATTGGTGCAAACAGAATATATCCTGGTGTTTCTGAAGCATTAAAATTTGCAAGCTCAAGGGTTTACATTGTCACCACAAAACAGAGCCGCTTTGCCGATGCTTTACTTCGAGAGCTTGCTGGAGTAACCATACCACCTGAAAGGATATACGGTCTCCGAACTGGTCCTAAGGTAGAAATTCTAAAGCAGCTTCAAAAGAGACCAGAGCATCAAGGGCTGAAACTGCACTTTGTTGAGGACCGGCTAGCTACGTTAAAAAATGTGATCAAAGAGCCCGAGTTAGACAATTGGAATTTGTATCTAGGGAATTGGGGTTACAACACTGCACAAGAGAAAGAGGAAGCTGCAGCTATTGCCAGAATTCGTGTTCTTCAACTCTCAGACTTCAGCAAGAAGTTGNAAAGTTAGGGTTTTYCTATTAG
TARGET=163,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04724 Ps_contig_mira-and-tgicl-ass_franssen_34432
SEQUENCE=AGAAACAGCAGAATGCAGGTAGCACCGGTGATAATAATCGGAGCCGGAACTTCCGGCATAGCCACGGCAGCTTCTTTAACAAAACAATCCATACCATTCATCATTCTCGAAAGAGAAAACTGTTTTGCTTCTCTATGGCAAAACTACACCTATGATCGTCTTCATCTTCATCTAAGAAAGCAAATTTGCGAGTTACCATATTTTCCATTTCCATCTTCTTATCCTAACTACGTACCAAAAAAGCAGTTCATACAGTATCTAGACAGCTATGTTAAAAACTTCAACATCGATCCTTTATACAACAGAAAGGTTGTGTTAGCGGAGTATTTTGAGGATGATGAGAAATGGAGGGTTGAGGCTGAGAATAGAAGTTCCGGTGAAGTTGAAGAATATTCTGGAAGGTTCTTGGTGGTGGCTAGTGGCGAAACGGCTGAGCCTCGTGTACCGGAAGTTTTAGGGTTGGAGAGTTTCAATGGGAAAGTGATGCATTCGACTGGGTATAAAAATGGGAGAGAGTTTAAAGATGAACATGTTCTTGTTGTTGGTTCTGGGAATTCTGGTATGGAGATTTCTTTAGATTTGGCCAATTTTGGTGCCAAACCTTCTATCATTGTTAGAAGTCCGGTTCATTTTCTTTCAAGGGATATGATGTATTATGCTGGTGTTTTGATGAGTTATCTGTCACTAAGCACAGTGGAGAAACTAGTTGTGATAGTTAGCAGAATTGTGTATGGAGATCTGAGTAAATATGGTATACCTTTCCCTAGTGAGGGTCCTTTCACAATGAAGAATAAGTATGGAAAATTTCCTATAATTGATTTGGGAACAGTTAAGAAAATCAAATCTGGAGAGATACAGGTGTTGGGAGCTGAAATAGAGAGTATAAGAGGTAACCAAGTGTTGTTCCGTGATGGAAAGTCCTACCCATTTGACTCCATTATATTCTGTACAGGCTTCAGCAGATCAACTCAAAAATGGCTTAAGGGAGGTGATGATCTTTTGAATGAGGATGGTTTTCCAAAGCCTGGTTTACCATACAATTGGAAGGGTAAGAATGGTTTGTACTGCGTTGGATTGTCAAGGAGGGGGTTTTATGGAGCTAACCTTGATGCTCAAAATGTAGCAAATGATATTGCCCTGTTAGTTCCTCAAGATGAAAGAAAATGAGTTGTGACTGTCATGTGATGTGCTTTGTTTGTTTGAAACGAGGAATGTGGTTTGGTGTGATTTAAGATGGACCATACAAGTGATGTTTAAGAACTAAATGATCATAATGTATAATTTGGTTACCACAATTATTGAATACTTTAGTTCTTGTTTATGATTATNGAAAAAATGTATGTTTAGTTTTTAATTTCTTTTCTCATTTCT
TARGET=1191,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5193
PRIMER_LEFT_PENALTY=0.002623
PRIMER_RIGHT_PENALTY=0.516652
PRIMER_LEFT_SEQUENCE=CAACTCAAAAATGGCTTAAGGG
PRIMER_RIGHT_SEQUENCE=AATCACACCAAACCACATTCCT
PRIMER_LEFT=964,22
PRIMER_RIGHT=1228,22
PRIMER_LEFT_TM=59.997
PRIMER_RIGHT_TM=60.517
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.5314
PRIMER_LEFT_1_PENALTY=0.014792
PRIMER_RIGHT_1_PENALTY=0.516652
PRIMER_LEFT_1_SEQUENCE=TGTAGCAAATGATATTGCCCTG
PRIMER_RIGHT_1_SEQUENCE=AATCACACCAAACCACATTCCT
PRIMER_LEFT_1=1118,22
PRIMER_RIGHT_1=1228,22
PRIMER_LEFT_1_TM=59.985
PRIMER_RIGHT_1_TM=60.517
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=0.5765
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.516652
PRIMER_LEFT_2_SEQUENCE=AGGGAGGTGATGATCTTTTGAA
PRIMER_RIGHT_2_SEQUENCE=AATCACACCAAACCACATTCCT
PRIMER_LEFT_2=982,22
PRIMER_RIGHT_2=1228,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=60.517
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.7025
PRIMER_LEFT_3_PENALTY=0.185822
PRIMER_RIGHT_3_PENALTY=0.516652
PRIMER_LEFT_3_SEQUENCE=GAATGAGGATGGTTTTCCAAAG
PRIMER_RIGHT_3_SEQUENCE=AATCACACCAAACCACATTCCT
PRIMER_LEFT_3=1001,22
PRIMER_RIGHT_3=1228,22
PRIMER_LEFT_3_TM=59.814
PRIMER_RIGHT_3_TM=60.517
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=0.7157
PRIMER_LEFT_4_PENALTY=0.199067
PRIMER_RIGHT_4_PENALTY=0.516652
PRIMER_LEFT_4_SEQUENCE=GAGGGGGTTTTATGGAGCTAAC
PRIMER_RIGHT_4_SEQUENCE=AATCACACCAAACCACATTCCT
PRIMER_LEFT_4=1082,22
PRIMER_RIGHT_4=1228,22
PRIMER_LEFT_4_TM=60.199
PRIMER_RIGHT_4_TM=60.517
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_04725 Ps_contig_mira-and-tgicl-ass_franssen_34439
SEQUENCE=CATACACCAACCTAACTCATGGAAAAAATAAGCATAGCACATGTATCATACATAAACAAGCCATGTCAAATGCAATACATAACCAAAATCTAATTACATGTTAATTTTCCAATCATCTAAGGCATGGACATAGAAACCACATTACAAATCCAAATAGCCTATACCATACACAATATTTTCCATACAACCATGACCAAAAGACTTAGCCTAGCACATCACTAAAACAATTCCAAATAGGTCACCAATACAAACAAGACCTAAACCTAACCTAACCATAATCAAAGCCTAAAACAGTCCAACATTGATCAACCAAGCAATATCAACCAAACCATAGCCCTGCAGTAGCAAAAACCTCCACCAAATCAATCATAATCATGCAGTATGATCCAAAATTCTGTAGCAAGCATGTGTACACAATCATCCACAAATTGAGCCAACAATTCAACATCAGCATATGTAGTTTAGTAGCCTGCACCATGAATGAACAAGGCAACCAGTTCACATTAAGAACACAACATTCGACTAACCTGACCTTACAACCAACCTGCAACAACCATCATTATGCAGCAGCCTATGCATATACAACCATATGCAATCCTGTATAACCCCAAAGTCCAGCAACAATACCAAAATCCAGAATTCCGCTGTTAGCTTCGACGTCTCCGATAGCGTAAGTTTCTCTTTCATCTTTTTGCGTCAACCGTGTTACCGAAATGTGAACGAAGGTGAGGGGAATCAAAGTCCCAACCTCTTGGGCTCCGATTCCCCGTGGTGAACATTTAATTTACCTTTTTCATAATTAGGGTTCTTCGACGGCTTGCATCGATTTAGGTTTTAGAAGTGGAATTAGAGAAAAATGGACATTGGATTTGGATAGAGTGCCTTGAAACAAGTTGAATGATGTTCTTGGTTTGATTTTAGAGGGGCCTCTGCCGCCGGAGACAGCCTCCGACACGGCGGAGGGAGAAACTAGAACGGAGGAGAGAGAGACGAGTTTGAACGCATGTGGTTGAATTTGAGTTTCACTTGTTTATCCNCGTGTTATTTTCATGTTAAACTTACCCCGAC
TARGET=981,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0935
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=1.059366
PRIMER_LEFT_SEQUENCE=TTGGATTTGGATAGAGTGCCTT
PRIMER_RIGHT_SEQUENCE=CACGNGGATAAACAAGTGAAAC
PRIMER_LEFT=863,22
PRIMER_RIGHT=1040,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=58.941
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.50
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=1.1731
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=1.139014
PRIMER_LEFT_1_SEQUENCE=TTGGATTTGGATAGAGTGCCTT
PRIMER_RIGHT_1_SEQUENCE=CACGNGGATAAACAAGTGAAACT
PRIMER_LEFT_1=863,22
PRIMER_RIGHT_1=1040,23
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.861
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.50
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=1.2012
PRIMER_LEFT_2_PENALTY=0.141882
PRIMER_RIGHT_2_PENALTY=1.059366
PRIMER_LEFT_2_SEQUENCE=CATAATTAGGGTTCTTCGACGG
PRIMER_RIGHT_2_SEQUENCE=CACGNGGATAAACAAGTGAAAC
PRIMER_LEFT_2=794,22
PRIMER_RIGHT_2=1040,22
PRIMER_LEFT_2_TM=59.858
PRIMER_RIGHT_2_TM=58.941
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.50
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.75
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=1.2809
PRIMER_LEFT_3_PENALTY=0.141882
PRIMER_RIGHT_3_PENALTY=1.139014
PRIMER_LEFT_3_SEQUENCE=CATAATTAGGGTTCTTCGACGG
PRIMER_RIGHT_3_SEQUENCE=CACGNGGATAAACAAGTGAAACT
PRIMER_LEFT_3=794,22
PRIMER_RIGHT_3=1040,23
PRIMER_LEFT_3_TM=59.858
PRIMER_RIGHT_3_TM=59.861
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.50
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.75
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=1.2962
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=1.262136
PRIMER_LEFT_4_SEQUENCE=TTGGATTTGGATAGAGTGCCTT
PRIMER_RIGHT_4_SEQUENCE=ACACGNGGATAAACAAGTGAAAC
PRIMER_LEFT_4=863,22
PRIMER_RIGHT_4=1041,23
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.738
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.50
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_04726 Ps_contig_mira-and-tgicl-ass_franssen_34441
SEQUENCE=AATGCGACTGAGAAACCAATGTAAGACAAAATAAAAAATAGAACCGCTTCAGTAACTTATCGTCTTTCCATTTCAAATTTCTCAAACACACCAACCGCTTTCTTGTTGCAGTTAGGTTGGTTGGTGTAGCCATGGCGTCTTCCTTCTCAACTCAATTACTTCAATCTCAGTACGCACTTTCTCATCTTCAGGGTAATTTTAGCCATGATGTGTCTCTGGTTCAAAGGTCACAAAAGGTATCACGGATTGGTTACAGTAAATCATTACCATCAAAATCACATTTTTCTTTGAAGTTTTCAACCACACAACGATCTGGACCAAGATCAATTAGATATAGGAGAGTTACATGTGCTGCTGCTGCTGCTGAAAATGTTCAAGAGCTGCAAGCCAAAGTGACTACCAAATGCTTCTTTGATGTAGATGTTGGGGGTAACGCTATCGGCACAATCGTTTTGGGCTTGTTCGGAGAGGTTGCTCCTAAAACAGTTGAAAATTTCCGCGCTTTATGCACAGGAGAGAAAGGATATGGTTACCAAGGATCCTCCTTCCATCGCATAATCAAAGAATTCATGATTCAGGGAGGGGACTTCACTGAAGGAAATGGAACCGGTGGAAAAAGTATCTATGGTTCTAGTTTTGAAGACGAGAGTTTTGCGTTGAAACATGTTGGTCCTGGAGTTTTGAGCATGGCTAATGCGGGTCCTAATACCAACGGTAGTCAATTTTTTATTTGCACTGTAAAGACTCCATGGTTAGATGATCGACATGTTGTATTTGGACATGTGATTGATGGAATGGATGTTGTGAGGACACTTGAGTCTCAAGAAACAAGCAGGTTAGATATCCCTACAAAGCCTTGCAGAATTGTTAAATGCGGAGAATTGCCTCTAGATGGTTGATACTTTTACGGATCTTCTGAATATCGTCTATTAATCATGTTTACGAGTTTGTATTATCGTCGGTTAATGGGTTGTCATTTGGAGACAAGGCAGAGATACAACACCCATGTTTGGTTTTACAGACTAATGTTAAATCCTTGTAATGACAATATTATCTTATCCACTTTTTTTTCTTTCTTTCTGTCTTTGTTATTTGCATTTACAAGCTATTTTCATCG
TARGET=350,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1478
PRIMER_LEFT_PENALTY=0.052187
PRIMER_RIGHT_PENALTY=0.095574
PRIMER_LEFT_SEQUENCE=GAAGTTTTCAACCACACAACGA
PRIMER_RIGHT_SEQUENCE=CCATATCCTTTCTCTCCTGTGC
PRIMER_LEFT=289,22
PRIMER_RIGHT=528,22
PRIMER_LEFT_TM=60.052
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.1617
PRIMER_LEFT_1_PENALTY=0.052187
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=GAAGTTTTCAACCACACAACGA
PRIMER_RIGHT_1_SEQUENCE=ACCCCCAACATCTACATCAAAG
PRIMER_LEFT_1=289,22
PRIMER_RIGHT_1=430,22
PRIMER_LEFT_1_TM=60.052
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=142
PRIMER_PAIR_PENALTY_2=0.2045
PRIMER_LEFT_2_PENALTY=0.108929
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=CAAAAGGTATCACGGATTGGTT
PRIMER_RIGHT_2_SEQUENCE=CCATATCCTTTCTCTCCTGTGC
PRIMER_LEFT_2=230,22
PRIMER_RIGHT_2=528,22
PRIMER_LEFT_2_TM=60.109
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=0.2177
PRIMER_LEFT_3_PENALTY=0.108206
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=GCGTCTTCCTTCTCAACTCAAT
PRIMER_RIGHT_3_SEQUENCE=ACCCCCAACATCTACATCAAAG
PRIMER_LEFT_3=134,22
PRIMER_RIGHT_3=430,22
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.2184
PRIMER_LEFT_4_PENALTY=0.108929
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=CAAAAGGTATCACGGATTGGTT
PRIMER_RIGHT_4_SEQUENCE=ACCCCCAACATCTACATCAAAG
PRIMER_LEFT_4=230,22
PRIMER_RIGHT_4=430,22
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=201
=
PRIMER_SEQUENCE_ID=SSR_04727 Ps_contig_mira-and-tgicl-ass_franssen_34441
SEQUENCE=AATGCGACTGAGAAACCAATGTAAGACAAAATAAAAAATAGAACCGCTTCAGTAACTTATCGTCTTTCCATTTCAAATTTCTCAAACACACCAACCGCTTTCTTGTTGCAGTTAGGTTGGTTGGTGTAGCCATGGCGTCTTCCTTCTCAACTCAATTACTTCAATCTCAGTACGCACTTTCTCATCTTCAGGGTAATTTTAGCCATGATGTGTCTCTGGTTCAAAGGTCACAAAAGGTATCACGGATTGGTTACAGTAAATCATTACCATCAAAATCACATTTTTCTTTGAAGTTTTCAACCACACAACGATCTGGACCAAGATCAATTAGATATAGGAGAGTTACATGTGCTGCTGCTGCTGCTGAAAATGTTCAAGAGCTGCAAGCCAAAGTGACTACCAAATGCTTCTTTGATGTAGATGTTGGGGGTAACGCTATCGGCACAATCGTTTTGGGCTTGTTCGGAGAGGTTGCTCCTAAAACAGTTGAAAATTTCCGCGCTTTATGCACAGGAGAGAAAGGATATGGTTACCAAGGATCCTCCTTCCATCGCATAATCAAAGAATTCATGATTCAGGGAGGGGACTTCACTGAAGGAAATGGAACCGGTGGAAAAAGTATCTATGGTTCTAGTTTTGAAGACGAGAGTTTTGCGTTGAAACATGTTGGTCCTGGAGTTTTGAGCATGGCTAATGCGGGTCCTAATACCAACGGTAGTCAATTTTTTATTTGCACTGTAAAGACTCCATGGTTAGATGATCGACATGTTGTATTTGGACATGTGATTGATGGAATGGATGTTGTGAGGACACTTGAGTCTCAAGAAACAAGCAGGTTAGATATCCCTACAAAGCCTTGCAGAATTGTTAAATGCGGAGAATTGCCTCTAGATGGTTGATACTTTTACGGATCTTCTGAATATCGTCTATTAATCATGTTTACGAGTTTGTATTATCGTCGGTTAATGGGTTGTCATTTGGAGACAAGGCAGAGATACAACACCCATGTTTGGTTTTACAGACTAATGTTAAATCCTTGTAATGACAATATTATCTTATCCACTTTTTTTTCTTTCTTTCTGTCTTTGTTATTTGCATTTACAAGCTATTTTCATCG
TARGET=1069,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04728 Ps_contig_mira-and-tgicl-ass_franssen_34450
SEQUENCE=CTTAATNAGCCANGTGACTCCCTTCATCATGTGAGGAAGAATAAGCAGATGAACTAGCCATAAATTCCAATTTGCCTAAATTGCTCAATGATCCAATCATGCATGCTTAAGCCATAACTAACTACTTTCATTTTCTGTTAAAAGATAGAACAGAAGTATACATAGGGTAAGGCATAGCTAATCTAGTGTTTTCAGTAATCAAAATATCCAAAGGACAGACAATGTCTTCATACATTATTTCTGAACTTCAAATTCACCGAGATTCCCAATCCTTCAATTTTTTGCTTCCTTTAGGCTTTGCTATAGGTGCATTCATTGCTGCCATTCTCTCGTTATACTTTGCACGTAGTGGATCATTGTAAGTCCACGGGTTGTTCCAATCAGTAGTGTCCTCATTGACCAGATGTGACCATTTAGTTCTTCCACTACGACCAAAGTGCTTGACTTGCATAACCTTAGGCAATATTGTCTTGTCCATTTTATCTTCCCCAGTTGGGGCTGAGAAATCACGTGTGAAAATATTGTCAGTTCCAGCAGTGGCGGCTCGATCATCAGAATTAGACTGGAAGAAAGCACCCTTGTGATAGTATTTCTGCATAAATCTCCATTTCTGTTTTGACGATTGAGAAGGTTTTGGATTCTTCCTTTCCCACTCTCTCCTTTCTTCCTCTGTCATGTTCCTCACCCTCTCAACCTCTTCTTTTTCCTTCAACAGAGCCTCCCGATCCTCTCTATCCCTCTTGATCCTACCAATCTCTCTCACTTTCCAAGCTTCATATTCCTCTGCCTCGTTGATTTCATCATCAGTATCCACATCAGCAATATTTGCCTCCAATTCAACATTTTTCTGGATTTCTTCATCCTTCCGTATCTCCTCAACAACAATCTGCTTCGTCTCAATCCTCCTTTGCTCCAATCTCCTTTTCCTTCCTTCCTCAAGAGCCTCCTCTTCCGCTTCAAGACGCTCACGCTCCGCAATGGTATCTCTCTCCGACTTGGGAACAAACACAGGCTTCACCATAGCTACTCCCGTATATTCCTCATCAGAATCAGTCTCATATTCTGACTCCTCTTCCTCCTCTTCTTCCTCCTCCTCCTCTTCCTCTTCCTGAGGAAGAGCCTCCTCTTGCTCTCTCTGAAGCAACTTCTCCTTAAGCATTCTCCTTCTCTCCGCCATAGCATCCTCATCTTGTTCTTCCAAATCCAACCCCCTCCTGCCTCCTCGCCTCCTCTTCAATGGTAGAAACAATCTCAGCCTGCCTAATACGGCGATGATCCGCTCTAACTTCCTCACGGTTATCTATTCGACTCTCGGCCAATCTACGAAGCCTACGATCATCTTTCCTAACAATAGCAGTATCTTCTTCATGACGAGGAAACGCTTTATCCAAAGCAGCTTCCCTGGTGGACCTAATATCACCAGAACCATCTTCATTCTCCTCATCAGCCCATTCAGGAACTTTACCAGGCCAATAACGCTTAACTTTTGTTTGGCCAATTTTACCTCTAAGCTTATCCCTTACAGCTATTACCGTATCACTAACACCTGCTGTCACTGACATTGTTCTTCGAAATCAACACCAACAGGAGAAAGCTAAATAACAAATTTTGATCCCTTTCTCCTTCAAACCCTCACGACGCAAGAATAACTATCTGCGGCAACAGAGACGGTGGTGAGGCGGAGGCGGAAAGTATGGTGACCGGGAACCTCTGTTTCTTTCYTCCNTTCGGGGCAGCGTCGTCGCTCTCTCCCTGGTTATTCGGTTCTGTCCCCGTACTCTGCGTTGACTACCTA
TARGET=924,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04729 Ps_contig_mira-and-tgicl-ass_franssen_34450
SEQUENCE=CTTAATNAGCCANGTGACTCCCTTCATCATGTGAGGAAGAATAAGCAGATGAACTAGCCATAAATTCCAATTTGCCTAAATTGCTCAATGATCCAATCATGCATGCTTAAGCCATAACTAACTACTTTCATTTTCTGTTAAAAGATAGAACAGAAGTATACATAGGGTAAGGCATAGCTAATCTAGTGTTTTCAGTAATCAAAATATCCAAAGGACAGACAATGTCTTCATACATTATTTCTGAACTTCAAATTCACCGAGATTCCCAATCCTTCAATTTTTTGCTTCCTTTAGGCTTTGCTATAGGTGCATTCATTGCTGCCATTCTCTCGTTATACTTTGCACGTAGTGGATCATTGTAAGTCCACGGGTTGTTCCAATCAGTAGTGTCCTCATTGACCAGATGTGACCATTTAGTTCTTCCACTACGACCAAAGTGCTTGACTTGCATAACCTTAGGCAATATTGTCTTGTCCATTTTATCTTCCCCAGTTGGGGCTGAGAAATCACGTGTGAAAATATTGTCAGTTCCAGCAGTGGCGGCTCGATCATCAGAATTAGACTGGAAGAAAGCACCCTTGTGATAGTATTTCTGCATAAATCTCCATTTCTGTTTTGACGATTGAGAAGGTTTTGGATTCTTCCTTTCCCACTCTCTCCTTTCTTCCTCTGTCATGTTCCTCACCCTCTCAACCTCTTCTTTTTCCTTCAACAGAGCCTCCCGATCCTCTCTATCCCTCTTGATCCTACCAATCTCTCTCACTTTCCAAGCTTCATATTCCTCTGCCTCGTTGATTTCATCATCAGTATCCACATCAGCAATATTTGCCTCCAATTCAACATTTTTCTGGATTTCTTCATCCTTCCGTATCTCCTCAACAACAATCTGCTTCGTCTCAATCCTCCTTTGCTCCAATCTCCTTTTCCTTCCTTCCTCAAGAGCCTCCTCTTCCGCTTCAAGACGCTCACGCTCCGCAATGGTATCTCTCTCCGACTTGGGAACAAACACAGGCTTCACCATAGCTACTCCCGTATATTCCTCATCAGAATCAGTCTCATATTCTGACTCCTCTTCCTCCTCTTCTTCCTCCTCCTCCTCTTCCTCTTCCTGAGGAAGAGCCTCCTCTTGCTCTCTCTGAAGCAACTTCTCCTTAAGCATTCTCCTTCTCTCCGCCATAGCATCCTCATCTTGTTCTTCCAAATCCAACCCCCTCCTGCCTCCTCGCCTCCTCTTCAATGGTAGAAACAATCTCAGCCTGCCTAATACGGCGATGATCCGCTCTAACTTCCTCACGGTTATCTATTCGACTCTCGGCCAATCTACGAAGCCTACGATCATCTTTCCTAACAATAGCAGTATCTTCTTCATGACGAGGAAACGCTTTATCCAAAGCAGCTTCCCTGGTGGACCTAATATCACCAGAACCATCTTCATTCTCCTCATCAGCCCATTCAGGAACTTTACCAGGCCAATAACGCTTAACTTTTGTTTGGCCAATTTTACCTCTAAGCTTATCCCTTACAGCTATTACCGTATCACTAACACCTGCTGTCACTGACATTGTTCTTCGAAATCAACACCAACAGGAGAAAGCTAAATAACAAATTTTGATCCCTTTCTCCTTCAAACCCTCACGACGCAAGAATAACTATCTGCGGCAACAGAGACGGTGGTGAGGCGGAGGCGGAAAGTATGGTGACCGGGAACCTCTGTTTCTTTCYTCCNTTCGGGGCAGCGTCGTCGCTCTCTCCCTGGTTATTCGGTTCTGTCCCCGTACTCTGCGTTGACTACCTA
TARGET=1086,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04730 Ps_contig_mira-and-tgicl-ass_franssen_34453
SEQUENCE=GTGTCGTTTCTTCGGCACAGGAGTGTCGTGATCATGTACACCCTTGTAAGTTATGATGACAGCATCTGAGTTGTCTACGGCTGTCTCGATGTGCTTCCGGACAGGACATCCGGCAGAGGTGCATCTGTAGTAGTTTCTGGGATGTGGGTTACCCTTCACCATTTTCTGCCCATACTTGCGCCATCGGTATCCATCACCTGATATCCCCACATCTCCGGCTGCATGTACAACAAACTTGGGTTTCTTTCCAGGTTTTACAGGAGAATCTGGTTGAATTAAGTCGCCTTTCTCCTTTCTTCGTTTTGGCTCTGGCTTGTCTAGATGTTCCTCCTTCAAAATAACTTTACCATTACCATTGATATTAGACGAGTTTTGTGGTTCCTTGTCAGCACTATAAGGTGCTTCTTGAGCTTTGGAAGATGTGGACGGATCAGAATCTTTCAGAACATTGACATACTTCACTAAAACATTGTTCTCTGCAGTAGGTTCTTTGGGAGATGCAAACTTACTTTCCCTAAGGGAAAGAGTCTTCCGCGGGGGATCATGACTGTGCCCACTTTTATAAACAGTCTCTATCACATGACCTGAATGATCCCAGCATTCAATCTTCTTAGCACAACAGTTTGATTGAGTACACCTGTAATAGCTTCGAGAACCTGTAGTAGGACTCTTAACTTGCTTCTGACCATATTTCCGCCAGTTGTATCCATCAGATGCTGATGTCCTCGCAACAGAAACTGAAGATAAGGCTTTACCGCCAGAAGGATATTTTTTGTCAACTACTGGTACAGGTGTGTTACTAACRCTTGGAGGAGACATTTTATATATTTGTATGGTTGAAATAGAAACAGGTGAAAAAGATTTTGTAACAGAATTTGGTGGTATTTCAGTCAGTGGAGTTGAATACAGAATCATTGCAAGCTGATTTTCAGTCGGAGTTGGAGGTGTCGGGGCTGCTTCACTTGCAGTAATTTCCTTTTCAGGAGGTCCAACTGTTTCCTTATTTTCAGCTTTATCACACGAATTCAGCGAATTAGGGATCCCCTGCAAATCTGCGTCGAAACTCTGTTTCTGCAAAATCGAAGATGGAGGAACAATTAGGGTTTCTGAATTTCGCACTACCGAGTGTTCAATAGAACTCGCTGAATCGTTTTGTTTAGACTCGGTGACACGTGGAGATTCACCGACTCGTTTTTTTTCGTGTTGGTCTCTTTCATCGTTATTTCCAAGCTGAGTGGGTCCCACTGGGAGAGGTTCGGAGACGCGGTGCTCAGCCATGGAAAAAAACGCAGAGGAGAAAGAAAGGGTTATATTTCTATGGTTTGTTAGAAAAATGAAACGAGTTAAGGGTTTGGGGAAACGGTTTTGTTGTGAGTGTGTTTGAGTTTGACTAGTGTTGACTCACTCACTCACTACTCACTACTCACTACTCAGGTGTTTGTTTCTCGTTTCA
TARGET=1400,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04731 Ps_contig_mira-and-tgicl-ass_franssen_34461
SEQUENCE=CGTTGAGCAAATAAATGCAATTTATCAACTAAGATTCAGTCACAAGTAGAGTTCGGATGCGAGAAAATGTTTGGCACCTAATATCCAAACAAACACTATATAATAACAAAATGACATTCGACAAAACAAACCATACAATTCAAGACATTATGCTCTCCAGCTCTGATAAGGAACCACATATAAGCATTTCTTGATCCAAAAATGGACTTTCTCTGAACTCTCACAAGCCAGTAAAATGTCGAGTACCTTCAGTATTTGGCTCACAAGCATGAAAAGTCTCGATCTTTTACAGGCCAAGCACCGGGGCAAACTTTGAAAAAGTTATGACTTGTTTATGAGCTACTGCTTCTCGCTCTCCACGGTGAGCCTCGCTTTGATGATTTGGGTGCTTGTTTGTTACTCCTGTTTATTAGTAGTCTTCGTTTATGTTGTTTTTCCAGAAAAAGTTTCCGAACTTGTTTCCTCTTTTCTTCTACCTTCAATTTGGAGAGACTAGGCCGGTATAAAATAAGGGTTCGACCAATCCGATCAACGATCACTGAGCCAGTTGCTTCTTCCAACTGCTTCATAACATCATCTAACTCACCTGGACAACTCCCATGTATTTTAATCTTGAGAAGTTCATTAGCTTCAAGGTTATCAGTGAAAGCGGTAACAAGATTGGGGGTAACACCAGATTTACCCACCAACTGTGTATTAAGCTTTTTCCCCAAACTATGTGCATAAGACGCAAGTTCTTTCCTTTCCTTCACACTTAAACTCGGCATTTTCAACTTCAATCTCTCTTCCCTCTTTCCCACCAAGGGCGAAGAAGTCGAATCCACCACTTCTGTATCTTCGACACTGTCTCTTTCAGTGAAACCAGATTCTTCATCGTACTCTTCTTCTTCTTCCGATGCTTCTTCTGTGGAATTCGGAGATGAAGCTCCAATTACGGCGGAGAGGGGAGGGGTGTAGGAAAAAGCGCGGGAAGAAAAGGAGGGTGTGGATAAGAAAGAGTGGGGAAGGGTTTTGGGAAGAGGTGCATGACGGTGAAGTTTTCGAAGGGTAATATTGGAAGAAGGAAGAAAGAAAGGTTTGAGAAATGAAAGGAAAGAGGAAGACGGTGACAGTGACGGTAACGGCGGTTGTGAACGAAGAACAGGGAGGAGGTAACAGGATGCTGTTGCCGCTGCCGTCATGTCTGTTTGGATTCCCCGCGTACTCTGCGTTGAAATAACCAACTAGA
TARGET=880,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0933
PRIMER_LEFT_PENALTY=0.018835
PRIMER_RIGHT_PENALTY=0.074466
PRIMER_LEFT_SEQUENCE=AACACCAGATTTACCCACCAAC
PRIMER_RIGHT_SEQUENCE=ATCTCCGAATTCCACAGAAGAA
PRIMER_LEFT=668,22
PRIMER_RIGHT=920,22
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.0943
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=GGTAACAAGATTGGGGGTAACA
PRIMER_RIGHT_1_SEQUENCE=ATCTCCGAATTCCACAGAAGAA
PRIMER_LEFT_1=650,22
PRIMER_RIGHT_1=920,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=0.1492
PRIMER_LEFT_2_PENALTY=0.074695
PRIMER_RIGHT_2_PENALTY=0.074466
PRIMER_LEFT_2_SEQUENCE=ATCTCTCTTCCCTCTTTCCCAC
PRIMER_RIGHT_2_SEQUENCE=ATCTCCGAATTCCACAGAAGAA
PRIMER_LEFT_2=778,22
PRIMER_RIGHT_2=920,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=143
PRIMER_PAIR_PENALTY_3=0.1741
PRIMER_LEFT_3_PENALTY=0.038090
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=AAGTCGAATCCACCACTTCTGT
PRIMER_RIGHT_3_SEQUENCE=CCACTCTTTCTTATCCACACCC
PRIMER_LEFT_3=811,22
PRIMER_RIGHT_3=1001,22
PRIMER_LEFT_3_TM=60.038
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=0.1741
PRIMER_LEFT_4_PENALTY=0.038090
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=AAGTCGAATCCACCACTTCTGT
PRIMER_RIGHT_4_SEQUENCE=CCCACTCTTTCTTATCCACACC
PRIMER_LEFT_4=811,22
PRIMER_RIGHT_4=1002,22
PRIMER_LEFT_4_TM=60.038
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_04732 Ps_contig_mira-and-tgicl-ass_franssen_34474
SEQUENCE=AAACAATTGATTTAAAAGATCTACGGTTTAACTTAATTGTTAAAGCATGGAAGAAAATGTCTNACTACTTACAGAACAACACTTCTATATTACGAGCACATAATAGTAATACAAATCACATATATGTATACATATTTTTCTTCATAGAGCTTATAAATCATATGCGGATATCTTAATAATAGGCTTTAATTGATTCAACGATTATGTGATGAATGACTCTGTCTCTATAAAGACAAATCAAGCTTTTGAAATTGAATTAATCAACCTGTTGCTAAAGTCCCAAAGTTTTCTTCCTAAAATTCCATTTGAAGCAAATGTACTTGGTTTAAACTCATTACAATCCAGAAAGTACTTCCCAGATACCCCTTTCAAGCCTGGATGCAGTGCAACATAGCATGTTGTTGCAGCTCCCTGTGGGACATTCTTCCATATGAAAAAAGTGAACATCTTTTCAAGAAATTCATTAGTAAAGAAGAGTGCCTCATAAGAGGAGTCATAATTACTCCCGGGTGTACCGAGTTAGCTGTGATGTTGACACCTTCTTCCTTCAAGCGACGAGAAAGCTCGTTCGCGTGTAATATGTTAGCTAACTTGGATTGTCCATAAGCCTTCTTGTCAGAGTAACCGTTTTGATCATTGATATCGTCCAATCGAATGCCTTCTTCGTAAGTATAGGTATGAGCAATTGATGAGAGGTTTATGATTCTTCCCTCGATTCCGGTTGTTTTTGCAGTTTGTTTCATTTTGTCGAGTAGAAGATTTGTTAACAGAAAATGACCAAGATGATTTGTTGCAAATTGCATCTCAATTCCATCTTGTGATAGTTGGAATGGACAGAACATCACTCCAGCATTGTTTATTAAGATGTTGAGAGGAAGATCAAGAGCGAGGAAATTTTCCACAAACAATCTAACAGACTTCACTGAACAAAGATCAAGCTTCATTATGTCAACGCGAGCAGATTCGTTGTCTTGGAGGATAATCTGTTTAGCTTCCTTTGCAGATTCCATGTTTCTTGCTGCAATGATGACATGCGCCTTTCGAAGACTCAACACTCTTGCTGTCTCCAAGCCTATACCACTTGCTCCTCCTGTGATAATTGCAGTGAGGTTTCTGGCATCAATTCCTTCTGTCACCTGTTCCGCAGTTGTAGAGGATCCAAATCCGCTTGGTCCGGACATTCCGGTAACCAACGAGAAAATACCCACCATTATGATTTTTGAAGGTAGCACGCAAATCAAGAAAAAGTGAAATTAATATGTGAGCTTAAATGAAGAAGAAGAAGAAGTGGTTTGATGTGTTTATNNTGCATGTTAGGAAAATGTCTTTATTTATATTGGCAAAAGAGGTAAAATAATGAATTGATAGAAGAAGAAATGAGGAAGGTATAGCATGGGGCTAGGGAAGAAAAGTGTGAATCCCCGGCGTACT
TARGET=1273,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3466
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=0.237699
PRIMER_LEFT_SEQUENCE=CAACGAGAAAATACCCACCATT
PRIMER_RIGHT_SEQUENCE=TTCACACTTTTCTTCCCTAGCC
PRIMER_LEFT=1190,22
PRIMER_RIGHT=1417,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=59.762
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=0.4650
PRIMER_LEFT_1_PENALTY=0.227281
PRIMER_RIGHT_1_PENALTY=0.237699
PRIMER_LEFT_1_SEQUENCE=CGGTAACCAACGAGAAAATACC
PRIMER_RIGHT_1_SEQUENCE=TTCACACTTTTCTTCCCTAGCC
PRIMER_LEFT_1=1183,22
PRIMER_RIGHT_1=1417,22
PRIMER_LEFT_1_TM=59.773
PRIMER_RIGHT_1_TM=59.762
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.8000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.4650
PRIMER_LEFT_2_PENALTY=0.227281
PRIMER_RIGHT_2_PENALTY=0.237699
PRIMER_LEFT_2_SEQUENCE=CCGGTAACCAACGAGAAAATAC
PRIMER_RIGHT_2_SEQUENCE=TTCACACTTTTCTTCCCTAGCC
PRIMER_LEFT_2=1182,22
PRIMER_RIGHT_2=1417,22
PRIMER_LEFT_2_TM=59.773
PRIMER_RIGHT_2_TM=59.762
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.7352
PRIMER_LEFT_3_PENALTY=0.497481
PRIMER_RIGHT_3_PENALTY=0.237699
PRIMER_LEFT_3_SEQUENCE=ATGATTTTTGAAGGTAGCACGC
PRIMER_RIGHT_3_SEQUENCE=TTCACACTTTTCTTCCCTAGCC
PRIMER_LEFT_3=1212,22
PRIMER_RIGHT_3=1417,22
PRIMER_LEFT_3_TM=60.497
PRIMER_RIGHT_3_TM=59.762
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.7403
PRIMER_LEFT_4_PENALTY=0.502561
PRIMER_RIGHT_4_PENALTY=0.237699
PRIMER_LEFT_4_SEQUENCE=CGCAGTTGTAGAGGATCCAAAT
PRIMER_RIGHT_4_SEQUENCE=TTCACACTTTTCTTCCCTAGCC
PRIMER_LEFT_4=1142,22
PRIMER_RIGHT_4=1417,22
PRIMER_LEFT_4_TM=60.503
PRIMER_RIGHT_4_TM=59.762
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_04733 Ps_contig_mira-and-tgicl-ass_franssen_34477
SEQUENCE=GCAGTATTTTGAACAACTTCTTTACCAGAGGAGGAACCATTCGTAAGCAATGTTTCGAATTAAAAGCATTACTGTCAAATAATTATCAGACAATACACAGTACACAATGAACTCATTCAAATATACAACGTACCAACTTGCATGCTGCAAACTTGAACCATCCATAGTTTCCACCCGAGCCTTGCTTTTAGAAGAGACAAAACAACGTTTTATTTTTAGTTGGAGGATGTTCACCAGAGAGACCTTGATTAACTCAAATCGGAATTTGGAAGGTATAGCAGAACAACGTTCCATGGCTACCAACGGAGAGATGTAGCAATCTATCACGGTTGTGCAAAGCAAAGCAAAGCATTTGTCAGAATCTGAAATTCTTCTTCATGTATAGAACCACCTCTTGAATGAAACAGAGCAGAATGCATCCAGTTGCTCCAGGATACAAAACATACCAGAAACTCAAGTGTTGCAACTTGACACCGTCAATGAAAAGCAACGGCATGCTTACGGCTGGAGGGTGAACGGAGTCGGTGTAAATCATGTAGGCAACACAAGCTGCAACACTAGCACTCTTGGCAAGCCATCCAGGTCCAAATATAGTAAACGCCAAAACACCTATGGCTGCGCAACCTATTTGAGCCATCAACATACTGTACTTCCGTGCGGAAGGGGCTGAAGGTGAGGCAAATAAGAGTGCGGAAACTGCTCCTAATGGTGCAACTGTGATTGAGAGTCCCTTTGGTGTCAATAACTGATCCAGTTTTCCCAATATGGCCACCGCTAGAAATGCCCCTGCAGAAGGCCATAGAATATCGCTGAAGGAAGGAGTGGAAGAGGATTTGGGAGGTTTCCAATTCTCCCATATGGATGGTGAAACGACGTCGCTTGATGCCTTAATAATCACATTCCTTCTGGTGTTACTAAAGCCCCAATAACCTAACACCAAACCCTTGTTCATCTTCTTGTTCTGTGTAATGGCGAAGCTGTTAAAAGATTGTGGTAATGATAGTGGTGCCATAAATTTGGAAGTACCGCCGCATGCAATTATAGATGACATTTTGAGTAGTTGATTGTTCATCTGAAAACTCAAACTCAGAAACCCTTCCCTCTCTAATCTCTACCCAAGAGTACTATACTAAATATGTGTGACATCACAGGGTCGCAAGAGTAACTGGAAAAGACACGACCATTGAGAGAAATGTTTGGACTCAAATATTAGCCTTTCAAAACCCTCAAAGGTCACCCACCCCT
TARGET=334,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1272
PRIMER_LEFT_PENALTY=0.016290
PRIMER_RIGHT_PENALTY=0.110942
PRIMER_LEFT_SEQUENCE=CGGAATTTGGAAGGTATAGCAG
PRIMER_RIGHT_SEQUENCE=TGCCTACATGATTTACACCGAC
PRIMER_LEFT=259,22
PRIMER_RIGHT=541,22
PRIMER_LEFT_TM=59.984
PRIMER_RIGHT_TM=59.889
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.1336
PRIMER_LEFT_1_PENALTY=0.016290
PRIMER_RIGHT_1_PENALTY=0.117281
PRIMER_LEFT_1_SEQUENCE=CGGAATTTGGAAGGTATAGCAG
PRIMER_RIGHT_1_SEQUENCE=TACATGATTTACACCGACTCCG
PRIMER_LEFT_1=259,22
PRIMER_RIGHT_1=537,22
PRIMER_LEFT_1_TM=59.984
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.1365
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=0.110942
PRIMER_LEFT_2_SEQUENCE=ACCAACGGAGAGATGTAGCAAT
PRIMER_RIGHT_2_SEQUENCE=TGCCTACATGATTTACACCGAC
PRIMER_LEFT_2=298,22
PRIMER_RIGHT_2=541,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=59.889
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.1393
PRIMER_LEFT_3_PENALTY=0.025566
PRIMER_RIGHT_3_PENALTY=0.113753
PRIMER_LEFT_3_SEQUENCE=ACCAACGGAGAGATGTAGCAAT
PRIMER_RIGHT_3_SEQUENCE=AGAGTGCTAGTGTTGCAGCTTG
PRIMER_LEFT_3=298,22
PRIMER_RIGHT_3=566,22
PRIMER_LEFT_3_TM=60.026
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=0.1428
PRIMER_LEFT_4_PENALTY=0.025566
PRIMER_RIGHT_4_PENALTY=0.117281
PRIMER_LEFT_4_SEQUENCE=ACCAACGGAGAGATGTAGCAAT
PRIMER_RIGHT_4_SEQUENCE=TACATGATTTACACCGACTCCG
PRIMER_LEFT_4=298,22
PRIMER_RIGHT_4=537,22
PRIMER_LEFT_4_TM=60.026
PRIMER_RIGHT_4_TM=59.883
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_04734 Ps_contig_mira-and-tgicl-ass_franssen_34478
SEQUENCE=GTCGGGGTATTCTACTGGTTTTGTTTAGGTTTCGTGCTGCTGTTCACACTTTACAACAACAACAACAAAATGCAGTCAAGTCCTTTGCTTCTTCAGGAGCCTATTAGGATGGCTTCCATCCTAGAGCCATCCAAACCTACTTTCTTCAGNTATGACTAAAATTGTGGCACACTTGGTCCAAATGCGATCGGTTGATACAATACCCGNTGCCCGTCG
TARGET=53,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6146
PRIMER_LEFT_PENALTY=0.229480
PRIMER_RIGHT_PENALTY=0.385098
PRIMER_LEFT_SEQUENCE=TCGGGGTATTCTACTGGTTTTG
PRIMER_RIGHT_SEQUENCE=GTAGGTTTGGATGGCTCTAGGA
PRIMER_LEFT=1,22
PRIMER_RIGHT=139,22
PRIMER_LEFT_TM=60.229
PRIMER_RIGHT_TM=59.615
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=139
PRIMER_PAIR_PENALTY_1=0.8036
PRIMER_LEFT_1_PENALTY=0.418525
PRIMER_RIGHT_1_PENALTY=0.385098
PRIMER_LEFT_1_SEQUENCE=GGTTTTGTTTAGGTTTCGTGCT
PRIMER_RIGHT_1_SEQUENCE=GTAGGTTTGGATGGCTCTAGGA
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=139,22
PRIMER_LEFT_1_TM=59.581
PRIMER_RIGHT_1_TM=59.615
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.9876
PRIMER_LEFT_2_PENALTY=0.229480
PRIMER_RIGHT_2_PENALTY=0.758164
PRIMER_LEFT_2_SEQUENCE=TCGGGGTATTCTACTGGTTTTG
PRIMER_RIGHT_2_SEQUENCE=CCATCCTAATAGGCTCCTGAAG
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=111,22
PRIMER_LEFT_2_TM=60.229
PRIMER_RIGHT_2_TM=59.242
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=1.0520
PRIMER_LEFT_3_PENALTY=0.229480
PRIMER_RIGHT_3_PENALTY=0.822517
PRIMER_LEFT_3_SEQUENCE=TCGGGGTATTCTACTGGTTTTG
PRIMER_RIGHT_3_SEQUENCE=ATAGGCTCCTGAAGAAGCAAAG
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=103,22
PRIMER_LEFT_3_TM=60.229
PRIMER_RIGHT_3_TM=59.177
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=1.0813
PRIMER_LEFT_4_PENALTY=0.696189
PRIMER_RIGHT_4_PENALTY=0.385098
PRIMER_LEFT_4_SEQUENCE=CGGGGTATTCTACTGGTTTTGT
PRIMER_RIGHT_4_SEQUENCE=GTAGGTTTGGATGGCTCTAGGA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=139,22
PRIMER_LEFT_4_TM=59.304
PRIMER_RIGHT_4_TM=59.615
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_04735 Ps_contig_mira-and-tgicl-ass_franssen_34480
SEQUENCE=AAAGTTAATAATATGATATCAAGATTATACAAAATATTAGGTAAAATATTAGGTAAACGGTACAACAAACTGCACCGTTACGCCTTTTTTGGATGGCAAAACCAATCCTCTTAAACACTACATTGTCATGAAATGTAAATTAACAAAAGCATAATAACAAACCCCCTTCAAATTTTAGGCTCACTATATAGACATCTCAAATTTCAATTTTGGGAATTAAGCATTAGTACTGACTCAAAATATACATGTATGCACTAGGTAATAATGCATTGAATTTTCTTTCAAAAAGTGACTTTTCCAACAAGTGGAAAAATCCAAAAGAGCACACAGAAGCGCAGGCGCGGCGGCTCTTCAAAGTTTTATTTTCAAAGGAAACATACTATCAAGGGAGGGTAGAGAACCTACAGGCATTAGTTAGTCTCTGGAGCAATTTCACTAACATCTAAGTCTACTATGTTCTCTTTCCCGAATATGGTCAAATGCACCGTCGCCATTTTGGTTTTGCGGCTCAGCTTCTTGAGGGTGCCTGCAAAACCTGAAAATGATCCGGATATAATTTGAACAGTGGAACCTGTGGTAAGTTTAACGTTTTTTTAACAGATGAAGTTTCTTCTGTGACTGTGAGTTGGTTAGATGTTTTTCTAGAGCCTCTTATAGGCTTAGAATCAACAATAGCTTTTGAAACATCTGAGTCTATCTCTGAATTCGGATTCCCAGAGATTACAGCAGTTTTCTTTTGCTCTTCCTCAAATGCTTTGTCAGCATTTTCTTGCTCCACTTTTGCCTGTTTGTAAATTGATTCCATATCAACCTCAGATACTGGCCTTGGTTTATTAATCATTTTCTTTGTATTTCCAACCTTGGATCCAATAAAGCCACCAACACCATCATACTCTTTTATGAAGTCATGCAATGGTTTGTTCAATACACATCTCAGAAAAATACAACCCGCGAAAAGAGGCTTTGGTTTTACGGAGATGGAACCATTCTTCAATTTCTTTTTTTCATGGATAACTGGAGCATATACCTTAAAGTCTGTATCAGGGAAAAACTTGGCCAACGAACGAGCGAGTGCTTCCGCAGTATACTCAGCCTTAAGCCGCGAAACGCGTACAACCCACCATTGAGGACCCAATTTCATGAGATTATCAAGGTTTAGTTCATCCATCCATGACTTCTTCTCCTTCTTAGTCTTCTTACTGAGTCTATCTTCTACTTCTTCTCTCCAATTGGTTGTGTTCTTGCTCTCTCTTCGCTCGTTTCTTAAGCGCCTTCTCTCTTTGGGAGTGAGGAGGTCTTGTTGTTGGGTGCCGGAAGAGGAGGCGGAGATTGAGAGAGGGAAGGGGGAGGAGAGAGAGGGAAGAGAGAGAGTGTGGAAGCGCGGTGGTGGATTCCATAGATGAAGCGTAAGCACCCTTTNATGTTCTATCTGAGAATCCCCGAC
TARGET=1362,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2845
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.276218
PRIMER_LEFT_SEQUENCE=TCTCCAATTGGTTGTGTTCTTG
PRIMER_RIGHT_SEQUENCE=AAGGGTGCTTACGCTTCATCTA
PRIMER_LEFT=1222,22
PRIMER_RIGHT=1417,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.276
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.2886
PRIMER_LEFT_1_PENALTY=0.012411
PRIMER_RIGHT_1_PENALTY=0.276218
PRIMER_LEFT_1_SEQUENCE=TTCTCTCTTTGGGAGTGAGGAG
PRIMER_RIGHT_1_SEQUENCE=AAGGGTGCTTACGCTTCATCTA
PRIMER_LEFT_1=1272,22
PRIMER_RIGHT_1=1417,22
PRIMER_LEFT_1_TM=59.988
PRIMER_RIGHT_1_TM=60.276
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.2886
PRIMER_LEFT_2_PENALTY=0.012411
PRIMER_RIGHT_2_PENALTY=0.276218
PRIMER_LEFT_2_SEQUENCE=CTTCTCTCTTTGGGAGTGAGGA
PRIMER_RIGHT_2_SEQUENCE=AAGGGTGCTTACGCTTCATCTA
PRIMER_LEFT_2=1271,22
PRIMER_RIGHT_2=1417,22
PRIMER_LEFT_2_TM=59.988
PRIMER_RIGHT_2_TM=60.276
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=0.3501
PRIMER_LEFT_3_PENALTY=0.073866
PRIMER_RIGHT_3_PENALTY=0.276218
PRIMER_LEFT_3_SEQUENCE=TCCATCCATGACTTCTTCTCCT
PRIMER_RIGHT_3_SEQUENCE=AAGGGTGCTTACGCTTCATCTA
PRIMER_LEFT_3=1163,22
PRIMER_RIGHT_3=1417,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=60.276
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.4795
PRIMER_LEFT_4_PENALTY=0.203324
PRIMER_RIGHT_4_PENALTY=0.276218
PRIMER_LEFT_4_SEQUENCE=TTGTGTTCTTGCTCTCTCTTCG
PRIMER_RIGHT_4_SEQUENCE=AAGGGTGCTTACGCTTCATCTA
PRIMER_LEFT_4=1233,22
PRIMER_RIGHT_4=1417,22
PRIMER_LEFT_4_TM=59.797
PRIMER_RIGHT_4_TM=60.276
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_04736 Ps_contig_mira-and-tgicl-ass_franssen_34493
SEQUENCE=AGGNAGCGTGAATTTTGCAGGGAAAATGTTGATTTGATTAAGAGTTATCCAAATGGAAAAATGATCCTAGTAGAATAGAAATCGGAAAGGGATGAGTAGCATGGCCATGGCGTGTGGATGTTGCTCCCACTACTACTGCCCTTCACAACACCCACTCTTCAATAATTCATCATCCTCTTCCTCCTCCTCATCCTCACAGTTGAAGCGTCCTACTACTGTGGTGGTTTCTCTTTCCAAATCCAACAACAATGGCGAAATTGATAAATCCGATTCTATTAAAGGATCTGGAACCACCGCTAGAGGTCGAAGATTGCTTAAAGTTCGACAAGACAAACTACAACGCGAACACGAACGAGTTCATAATTACCCTGCTTGGGCTAAAGTCCTTGAAGATGCTTGCAAAAACGATACCGAACTTCGTTCTGTACTTGGTGATAGCATCGGAAACCCTCAACTCATGAAGGAGCGGGTGGAAGATAGAATTCGAAAGAAGGGTAAAGACTTTCGCAAGTCCAAAACTGGATCTGTTGTAGCCGCTAAAGTCACCTTTAGAGACTTCGATCCACTTGATTCGTTTATCTGGTTTGAATTGTATGGATCACCCACCGATCGCGACGTTAATCTTATTGGCAGTGTTATTCAAGCTTGGTATGTCATGGGTCGAATTGGTGCCTTCAATTCTTCAAACTTGCAGATATCAAATTTGTCAATGGAGCACGATCCTCTCTATGATTCAGATAAGGGTTTCAAAGTGATGCCATCATCTTTCCATGATATCAGTGACGTCGAATTCCAGGATAATTGGGGACGTGTATGGGTGGACCTGGGTACGGCTGATTATTTTGCCATTGATGTGCTCCTCAACTGCCTAACTTGTTTAAGTTCAGAATATCTTGGCATCCAACAAATCGTGTTTGGTGGTCGGAGCATGGGTGATTGGGAAGAGGGGATGACGAACCCTGACTATGGTTACAAGTCCTTCAAGATATAAACAGGGATTGGATGCAGTTTATCCCTTCACTCGTATTTTGGTTCGAAAAATAACAGTGCAGGGAGGCAGTAACTATATGCATTGATAAGAACACGTCCTTGGGTGTTATGGTTAGTTTTAACTCGCAAAGTTGTACTTGGTTGTCACTTGTCCAGTAGGTTTCTTGCACTGTATGGAGCCAAAAGCCTTGAGATTCAACTGTGTTGTGTTGTGTTGTGACTGACATCAGAAATGATAGATTAGTGAGACAGTAGAATAGGAGCCGCTTGTGAAGCTTTTTTCATTTCAAATATCATGTTAGAATGATATATCAAAATATAGGTAGATCTATCATAAAATTAGTTTTGATATATGGTGATATGAAATGTAAAACAAGCATATTGAGTACTTAGTAATGTTCGTATTCTTGACTAAACTATGTTATCTTGTTGCT
TARGET=1191,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0671
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=GAAAAATAACAGTGCAGGGAGG
PRIMER_RIGHT_SEQUENCE=CACAAGCGGCTCCTATTCTACT
PRIMER_LEFT=1035,22
PRIMER_RIGHT=1261,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.0671
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.066394
PRIMER_LEFT_1_SEQUENCE=GAAAAATAACAGTGCAGGGAGG
PRIMER_RIGHT_1_SEQUENCE=ACAAGCGGCTCCTATTCTACTG
PRIMER_LEFT_1=1035,22
PRIMER_RIGHT_1=1260,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.1950
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=CCCTTCACTCGTATTTTGGTTC
PRIMER_RIGHT_2_SEQUENCE=CACAAGCGGCTCCTATTCTACT
PRIMER_LEFT_2=1013,22
PRIMER_RIGHT_2=1261,22
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.1950
PRIMER_LEFT_3_PENALTY=0.128591
PRIMER_RIGHT_3_PENALTY=0.066394
PRIMER_LEFT_3_SEQUENCE=TCCCTTCACTCGTATTTTGGTT
PRIMER_RIGHT_3_SEQUENCE=ACAAGCGGCTCCTATTCTACTG
PRIMER_LEFT_3=1012,22
PRIMER_RIGHT_3=1260,22
PRIMER_LEFT_3_TM=59.871
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.1950
PRIMER_LEFT_4_PENALTY=0.128591
PRIMER_RIGHT_4_PENALTY=0.066394
PRIMER_LEFT_4_SEQUENCE=TCCCTTCACTCGTATTTTGGTT
PRIMER_RIGHT_4_SEQUENCE=CACAAGCGGCTCCTATTCTACT
PRIMER_LEFT_4=1012,22
PRIMER_RIGHT_4=1261,22
PRIMER_LEFT_4_TM=59.871
PRIMER_RIGHT_4_TM=59.934
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=250
=
PRIMER_SEQUENCE_ID=SSR_04737 Ps_contig_mira-and-tgicl-ass_franssen_34498
SEQUENCE=AACACAGCTTTTTTGTACCTGCGGCCTCGAGGTTTCGCAACTAGCTTATATAAAACGATGAAATAAAAGAGAGACCAAAGGATCTATTTTATCGAATATCGTTTTATCGAAGTAGTATCTTGCAATTACATGACATCAAATGTTATAGAATTACAATGGTCATAGACTCATAGCATTGCCCTACATTCGTTGTGACATATTCACAAACATGTTATATCCATAATTCAGTTGAATCAAATACCTTGAAGATCATCAATTCTTCAATGAATCAGTTCCATTTACTTTTTCCCTGTCATATTAGAAACCAATCCCTTGAAGAATTTCGCAGACTTTGTTATCGGTGAATTGATCGCCATCAGATTATACAAAACACTCTCAACTTGATCAATGGTAGGCCTTACCTTGAATGAAGAAACCACCTTCATTTCTTCACCTTCTTCAACAAGAACATTCCATGTCTCTCGACTCACAACCCCGTCTTTCACGCACTTAAAAATCACACCTTTTCTTTTGCTGAATAACCCTCTTACCTCTAATCCCATAAAAGAATAGACTACTTCAAAAGAATTCACAAAACCACTTAGATTGCCAAAGTTACTCTCTTCCTCAAATGCCCATTTTGGATTCATCATAACCACTGGCTTTGGATAAACCCCATCACTCACCATTTTCACCACAGACAGTTGTGAACTCTCTGGCACCAAAAATATCACCACATCAGCAGAATTCAACATTCTGAGGTCAGTTTTGGCAACTGAAACAATGTCAATGTGCTCAACTTGGTTGCTTGAATGTGACTCAAAGGCAACAACTGCAGCTTCTTTTAAGCTAGGATTAGGCCAAAGGATTAAGAACTTAATGATAGAATCTTTTCTTTTGATGGGTATATCAATGTCCCCAAAAATATCAAGAGCAAGTTTGGAAAGCGAATCTGGTGAGTCGTTAATGACCGGGATTTCGACCCGGTATTTGGGTTGTTTCAGTTTCTTGATTTTGCCTAATAGTTTGGTGTTGTTGAGTGGTTTCTCTAAGGCTGTTGAAAGGGATGTTTTGGCTTGAAGTATGGCATCTTCCTTTGAGATGGGTGGGTTAAGGGAAGAGAGTGAAGAGTGGATAATGGATGTGGATGTAGCTTTGAGGGGAACATTAGGATTTTGGGGGATGCATGATATGTTGTAGTGTTTGCATAAATAATGAGTATGAAATGGGATAGATGTAAATTTGTTATTGGAATATGCAGTTATGCATGGTGATGGCTTGGAAAATTTGGTAGATTGTGAATGAGTTGAGTGATGGTAGAATGACGATGCCATTTAACAAAGGAACAAGCTGTGTGTGTGAGAGAGATACTTTTGATATGAAACAGTTGTTCTAAATTTCTATCTGGATAAGATATCCCAGTCTCACAATAGTTCAACCAATGGT
TARGET=1331,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2420
PRIMER_LEFT_PENALTY=0.156479
PRIMER_RIGHT_PENALTY=1.085505
PRIMER_LEFT_SEQUENCE=GATGGCTTGGAAAATTTGGTAG
PRIMER_RIGHT_SEQUENCE=GGTTGAACTATTGTGAGACTGGG
PRIMER_LEFT=1251,22
PRIMER_RIGHT=1418,23
PRIMER_LEFT_TM=59.844
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=1.2622
PRIMER_LEFT_1_PENALTY=0.176730
PRIMER_RIGHT_1_PENALTY=1.085505
PRIMER_LEFT_1_SEQUENCE=TGAGGGGAACATTAGGATTTTG
PRIMER_RIGHT_1_SEQUENCE=GGTTGAACTATTGTGAGACTGGG
PRIMER_LEFT_1=1135,22
PRIMER_RIGHT_1=1418,23
PRIMER_LEFT_1_TM=60.177
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=1.3379
PRIMER_LEFT_2_PENALTY=0.252355
PRIMER_RIGHT_2_PENALTY=1.085505
PRIMER_LEFT_2_SEQUENCE=GAACATTAGGATTTTGGGGGAT
PRIMER_RIGHT_2_SEQUENCE=GGTTGAACTATTGTGAGACTGGG
PRIMER_LEFT_2=1141,22
PRIMER_RIGHT_2=1418,23
PRIMER_LEFT_2_TM=60.252
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=1.4604
PRIMER_LEFT_3_PENALTY=0.374887
PRIMER_RIGHT_3_PENALTY=1.085505
PRIMER_LEFT_3_SEQUENCE=TAGCTTTGAGGGGAACATTAGG
PRIMER_RIGHT_3_SEQUENCE=GGTTGAACTATTGTGAGACTGGG
PRIMER_LEFT_3=1129,22
PRIMER_RIGHT_3=1418,23
PRIMER_LEFT_3_TM=59.625
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=1.5987
PRIMER_LEFT_4_PENALTY=0.513182
PRIMER_RIGHT_4_PENALTY=1.085505
PRIMER_LEFT_4_SEQUENCE=GATGTAGCTTTGAGGGGAACAT
PRIMER_RIGHT_4_SEQUENCE=GGTTGAACTATTGTGAGACTGGG
PRIMER_LEFT_4=1125,22
PRIMER_RIGHT_4=1418,23
PRIMER_LEFT_4_TM=59.487
PRIMER_RIGHT_4_TM=59.914
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_04738 Ps_contig_mira-and-tgicl-ass_franssen_34501
SEQUENCE=GGAGTAGATTCTCCAATTTGAGACACCGTTTTTCTCTCCACTTATCATGTATGAACCTTCTGTTCTTGAAATACCAAATCCAGAATCACACTTAGTGTATATGCAGTCTTGAACATAAAAAGATGTGTAGTAGTTTATGCCAATAAAATCCAATCCTTTCTTGAGTTTCTTTTTCTCACAGGTAGAAAATTTAGGTAAGATGCTTCCAAGAACTTCCTCCATCTCTTTGGGGTATTTACCAAATATGATTGGGTCCAAGAACCAATTGAAAGTGAACGATCTTGCTCTTTCGGTAGCCAATTTATCCGCTGTAGCATTGCTCATTGGTTCATACCATTCATGTGAAAGCACTATACCAATTTTTCCTCTCTGCTCAACCTGGTATTTGGTTCTATAGATATCAACTGCAGATGCATGTGACAGGATAATATTATGGGCTGCAACAAAGGGCTCTTTTTCGGAATCACCTTCTTTGCAGTTTACCATTGCCAATGAGCCCGAGCAACGACTTGGAGGATATATACCTGAGCGGTAACCGAATGGCGCGACGTGATTTGGCTCATTGAATGTGACCCAATACTTAACTCTGTCACCAAATGTTTTGAAGCATAGGTCTGCATAAAATGCAAAATCTTCCTGTGATTGGGGACTTAACCATCCTCCATATCTGTCCTCTAGTTCTTGTGGGCTGTCATAGTGACTTAGAGTAACAAAGGGTTGAATACCTTTGAGCAGCAAGGCATCAAATAGTTTGTTATAGAAGTCAATGCCGGGCCAATTGATTTCTCCAAATCTACCCTTTGGTAGAACTCTTGTCCATGATATGGAGAGTCTGTAACTATTTACTTTCAAAGCTTCCAATAGATCTATATCCTCCTGGTATCTATGATAATGATCAACTGCTATATCTCCATTACTTCCATCTACTATTATCTTTGTATCTCCGTGAGTGAAGTTATCCCAATTACTTTGTCCCTTACCATTAATGTTATAACCACCTTCATACTGGTAAGAAGAAGAAGCAGTTCCAAACAGAAACTTCTTAGGGAATGGAGAGAGATCATCATCATCCACAGCACTTGAATGATGAAGCAGAAGCAACAATATCTCGAGAAACAGAGTCTTCAATAACAATCTTACCATCTTTTGGGGTTTAAATTAATGTCCTACATC
TARGET=1011,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2638
PRIMER_LEFT_PENALTY=0.241544
PRIMER_RIGHT_PENALTY=0.022302
PRIMER_LEFT_SEQUENCE=CCGTGAGTGAAGTTATCCCAAT
PRIMER_RIGHT_SEQUENCE=TCTGCTTCATCATTCAAGTGCT
PRIMER_LEFT=943,22
PRIMER_RIGHT=1095,22
PRIMER_LEFT_TM=60.242
PRIMER_RIGHT_TM=60.022
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.2785
PRIMER_LEFT_1_PENALTY=0.241544
PRIMER_RIGHT_1_PENALTY=0.036956
PRIMER_LEFT_1_SEQUENCE=CCGTGAGTGAAGTTATCCCAAT
PRIMER_RIGHT_1_SEQUENCE=TGATGATCTCTCTCCATTCCCT
PRIMER_LEFT_1=943,22
PRIMER_RIGHT_1=1065,22
PRIMER_LEFT_1_TM=60.242
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=123
PRIMER_PAIR_PENALTY_2=0.5185
PRIMER_LEFT_2_PENALTY=0.496229
PRIMER_RIGHT_2_PENALTY=0.022302
PRIMER_LEFT_2_SEQUENCE=TCTCCGTGAGTGAAGTTATCCC
PRIMER_RIGHT_2_SEQUENCE=TCTGCTTCATCATTCAAGTGCT
PRIMER_LEFT_2=940,22
PRIMER_RIGHT_2=1095,22
PRIMER_LEFT_2_TM=60.496
PRIMER_RIGHT_2_TM=60.022
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.5332
PRIMER_LEFT_3_PENALTY=0.496229
PRIMER_RIGHT_3_PENALTY=0.036956
PRIMER_LEFT_3_SEQUENCE=TCTCCGTGAGTGAAGTTATCCC
PRIMER_RIGHT_3_SEQUENCE=TGATGATCTCTCTCCATTCCCT
PRIMER_LEFT_3=940,22
PRIMER_RIGHT_3=1065,22
PRIMER_LEFT_3_TM=60.496
PRIMER_RIGHT_3_TM=60.037
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.6363
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=0.394711
PRIMER_LEFT_4_SEQUENCE=CCGTGAGTGAAGTTATCCCAAT
PRIMER_RIGHT_4_SEQUENCE=TGTTGCTTCTGCTTCATCATTC
PRIMER_LEFT_4=943,22
PRIMER_RIGHT_4=1102,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=60.395
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_04739 Ps_contig_mira-and-tgicl-ass_franssen_34501
SEQUENCE=GGAGTAGATTCTCCAATTTGAGACACCGTTTTTCTCTCCACTTATCATGTATGAACCTTCTGTTCTTGAAATACCAAATCCAGAATCACACTTAGTGTATATGCAGTCTTGAACATAAAAAGATGTGTAGTAGTTTATGCCAATAAAATCCAATCCTTTCTTGAGTTTCTTTTTCTCACAGGTAGAAAATTTAGGTAAGATGCTTCCAAGAACTTCCTCCATCTCTTTGGGGTATTTACCAAATATGATTGGGTCCAAGAACCAATTGAAAGTGAACGATCTTGCTCTTTCGGTAGCCAATTTATCCGCTGTAGCATTGCTCATTGGTTCATACCATTCATGTGAAAGCACTATACCAATTTTTCCTCTCTGCTCAACCTGGTATTTGGTTCTATAGATATCAACTGCAGATGCATGTGACAGGATAATATTATGGGCTGCAACAAAGGGCTCTTTTTCGGAATCACCTTCTTTGCAGTTTACCATTGCCAATGAGCCCGAGCAACGACTTGGAGGATATATACCTGAGCGGTAACCGAATGGCGCGACGTGATTTGGCTCATTGAATGTGACCCAATACTTAACTCTGTCACCAAATGTTTTGAAGCATAGGTCTGCATAAAATGCAAAATCTTCCTGTGATTGGGGACTTAACCATCCTCCATATCTGTCCTCTAGTTCTTGTGGGCTGTCATAGTGACTTAGAGTAACAAAGGGTTGAATACCTTTGAGCAGCAAGGCATCAAATAGTTTGTTATAGAAGTCAATGCCGGGCCAATTGATTTCTCCAAATCTACCCTTTGGTAGAACTCTTGTCCATGATATGGAGAGTCTGTAACTATTTACTTTCAAAGCTTCCAATAGATCTATATCCTCCTGGTATCTATGATAATGATCAACTGCTATATCTCCATTACTTCCATCTACTATTATCTTTGTATCTCCGTGAGTGAAGTTATCCCAATTACTTTGTCCCTTACCATTAATGTTATAACCACCTTCATACTGGTAAGAAGAAGAAGCAGTTCCAAACAGAAACTTCTTAGGGAATGGAGAGAGATCATCATCATCCACAGCACTTGAATGATGAAGCAGAAGCAACAATATCTCGAGAAACAGAGTCTTCAATAACAATCTTACCATCTTTTGGGGTTTAAATTAATGTCCTACATC
TARGET=1060,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2638
PRIMER_LEFT_PENALTY=0.241544
PRIMER_RIGHT_PENALTY=0.022302
PRIMER_LEFT_SEQUENCE=CCGTGAGTGAAGTTATCCCAAT
PRIMER_RIGHT_SEQUENCE=TCTGCTTCATCATTCAAGTGCT
PRIMER_LEFT=943,22
PRIMER_RIGHT=1095,22
PRIMER_LEFT_TM=60.242
PRIMER_RIGHT_TM=60.022
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.5185
PRIMER_LEFT_1_PENALTY=0.496229
PRIMER_RIGHT_1_PENALTY=0.022302
PRIMER_LEFT_1_SEQUENCE=TCTCCGTGAGTGAAGTTATCCC
PRIMER_RIGHT_1_SEQUENCE=TCTGCTTCATCATTCAAGTGCT
PRIMER_LEFT_1=940,22
PRIMER_RIGHT_1=1095,22
PRIMER_LEFT_1_TM=60.496
PRIMER_RIGHT_1_TM=60.022
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.6363
PRIMER_LEFT_2_PENALTY=0.241544
PRIMER_RIGHT_2_PENALTY=0.394711
PRIMER_LEFT_2_SEQUENCE=CCGTGAGTGAAGTTATCCCAAT
PRIMER_RIGHT_2_SEQUENCE=TGTTGCTTCTGCTTCATCATTC
PRIMER_LEFT_2=943,22
PRIMER_RIGHT_2=1102,22
PRIMER_LEFT_2_TM=60.242
PRIMER_RIGHT_2_TM=60.395
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=0.6363
PRIMER_LEFT_3_PENALTY=0.241544
PRIMER_RIGHT_3_PENALTY=0.394711
PRIMER_LEFT_3_SEQUENCE=CCGTGAGTGAAGTTATCCCAAT
PRIMER_RIGHT_3_SEQUENCE=TTCTGCTTCATCATTCAAGTGC
PRIMER_LEFT_3=943,22
PRIMER_RIGHT_3=1096,22
PRIMER_LEFT_3_TM=60.242
PRIMER_RIGHT_3_TM=60.395
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.6363
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=0.394711
PRIMER_LEFT_4_SEQUENCE=CCGTGAGTGAAGTTATCCCAAT
PRIMER_RIGHT_4_SEQUENCE=GTTGCTTCTGCTTCATCATTCA
PRIMER_LEFT_4=943,22
PRIMER_RIGHT_4=1101,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=60.395
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_04740 Ps_contig_mira-and-tgicl-ass_franssen_34511
SEQUENCE=CGTTAAAAAGGTAAAACGAATTAGTAATTACATTAAGTTTGTAATACATAAAGACAAAGAATACATATGATATGATATGATACATGTTTTTAATATNAACAAACTATAAGATATGAAATTGAGTTGTTGAATTTGGACCAACATGTTTATAAAATACATTGAAAATTTATTGTACTTCAATTTGGAAAGCCTGCTGCAAGCTTAGCCCTTAGATCCTTTCGTAATATTTTGCCAGAAGGTGACTTGGGAATAGCATCAATGAAGAATACTCGATTTATTCTTTTGTAAAACACCACCTGTTTAGCGACGAATTTTTTAATTTCATCCTCAGTTGTGTCGATATCATTATTCGATCTAACCACAAATGCAACTGGAACCTCACCAGCGGCTTCATCTTGCATTGGGACGACAGCAACATCGGAGATTTGAGGATGCGAGAGAATAATTGCTTCAAGTTCAGCTGGAGCAACTTGAAAGCCTTTGTATTTAATCAATTCTTTAAGCCTATCAACGATGAACAATTCATCATCATCATCAATGTATCCAATGTCACCAGTATGCAACCAACCTTCTTTGTCTATGGTTCTCAATGTTGCTTCTGGATCATTTAGATAACCTTTCATGATTTGGTCACCTCTAATACAAATTTCACCAAGGTTGATTACGAGACAAAGAAGAATCATTTTCTGGATCAACAAATCTTCATCTCAGCAATTTCTAACAACGGTTCCACAGCACCTGGTTTTACATCTATTGGTTTCTTTTGCAAATGAATAAACACATTAGTTAAACCAACTGGGACCTCGCCTCCGTCATCCCATATCCCTGTCCAAGTTTGG
TARGET=65,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04741 Ps_contig_mira-and-tgicl-ass_franssen_34511
SEQUENCE=CGTTAAAAAGGTAAAACGAATTAGTAATTACATTAAGTTTGTAATACATAAAGACAAAGAATACATATGATATGATATGATACATGTTTTTAATATNAACAAACTATAAGATATGAAATTGAGTTGTTGAATTTGGACCAACATGTTTATAAAATACATTGAAAATTTATTGTACTTCAATTTGGAAAGCCTGCTGCAAGCTTAGCCCTTAGATCCTTTCGTAATATTTTGCCAGAAGGTGACTTGGGAATAGCATCAATGAAGAATACTCGATTTATTCTTTTGTAAAACACCACCTGTTTAGCGACGAATTTTTTAATTTCATCCTCAGTTGTGTCGATATCATTATTCGATCTAACCACAAATGCAACTGGAACCTCACCAGCGGCTTCATCTTGCATTGGGACGACAGCAACATCGGAGATTTGAGGATGCGAGAGAATAATTGCTTCAAGTTCAGCTGGAGCAACTTGAAAGCCTTTGTATTTAATCAATTCTTTAAGCCTATCAACGATGAACAATTCATCATCATCATCAATGTATCCAATGTCACCAGTATGCAACCAACCTTCTTTGTCTATGGTTCTCAATGTTGCTTCTGGATCATTTAGATAACCTTTCATGATTTGGTCACCTCTAATACAAATTTCACCAAGGTTGATTACGAGACAAAGAAGAATCATTTTCTGGATCAACAAATCTTCATCTCAGCAATTTCTAACAACGGTTCCACAGCACCTGGTTTTACATCTATTGGTTTCTTTTGCAAATGAATAAACACATTAGTTAAACCAACTGGGACCTCGCCTCCGTCATCCCATATCCCTGTCCAAGTTTGG
TARGET=523,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0987
PRIMER_LEFT_PENALTY=0.003166
PRIMER_RIGHT_PENALTY=0.095574
PRIMER_LEFT_SEQUENCE=TCGATCTAACCACAAATGCAAC
PRIMER_RIGHT_SEQUENCE=GGTGACCAAATCATGAAAGGTT
PRIMER_LEFT=349,22
PRIMER_RIGHT=634,22
PRIMER_LEFT_TM=60.003
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.1279
PRIMER_LEFT_1_PENALTY=0.032347
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=CTAACCACAAATGCAACTGGAA
PRIMER_RIGHT_1_SEQUENCE=GGTGACCAAATCATGAAAGGTT
PRIMER_LEFT_1=354,22
PRIMER_RIGHT_1=634,22
PRIMER_LEFT_1_TM=60.032
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.1911
PRIMER_LEFT_2_PENALTY=0.095538
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=TAACCACAAATGCAACTGGAAC
PRIMER_RIGHT_2_SEQUENCE=GGTGACCAAATCATGAAAGGTT
PRIMER_LEFT_2=355,22
PRIMER_RIGHT_2=634,22
PRIMER_LEFT_2_TM=59.904
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.2285
PRIMER_LEFT_3_PENALTY=0.198196
PRIMER_RIGHT_3_PENALTY=0.030276
PRIMER_LEFT_3_SEQUENCE=GAGGATGCGAGAGAATAATTGC
PRIMER_RIGHT_3_SEQUENCE=TGTCTCGTAATCAACCTTGGTG
PRIMER_LEFT_3=427,22
PRIMER_RIGHT_3=670,22
PRIMER_LEFT_3_TM=60.198
PRIMER_RIGHT_3_TM=60.030
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.2381
PRIMER_LEFT_4_PENALTY=0.198196
PRIMER_RIGHT_4_PENALTY=0.039879
PRIMER_LEFT_4_SEQUENCE=GAGGATGCGAGAGAATAATTGC
PRIMER_RIGHT_4_SEQUENCE=AGACAAAGAAGGTTGGTTGCAT
PRIMER_LEFT_4=427,22
PRIMER_RIGHT_4=578,22
PRIMER_LEFT_4_TM=60.198
PRIMER_RIGHT_4_TM=60.040
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_04742 Ps_contig_mira-and-tgicl-ass_franssen_34514
SEQUENCE=AATACCTAAAAACATATTGTGATAGGTCAACCGTTAAATGGATCTCAGGTAACTAGGTAAGTTACCTGAGAGTAATCGTCTCCGCAGTTGCGCGCAAAGGATRCCTCAGTTTACCAAACAAAATTGATTGTGTTGCCGAGTTAGTTAGTTTGTTACTATCACTTGTTGAAAATGTTACAAACCATTTCGTGTACACAGAATCTGACTTAGTATATTGGTTTGGATTTTCATGAAGGTTCTAAGTGATCCACAGAAGAAATCAATTTATGATCAGTATGGAGAAAGTGGACTAAAAAATGGAATGTCACCGACGGATGAAGCTACAGCTTCGTATTTTCAAACTCGGGATGGCAGAAGATTTAGATTCAATCCCAGAAGTGCAGATGGCATCTTTGCTGAGGTTTTTGGCCATTCAAGCCCTTATGGAGGAATGGGAATGAGGGGCGGCGGCTGCAGAGGCATGAGGGGTCAGACGTGGGTGTCGAGGTCTTTTGGTGATATGTTTGGTAAAGATGTATTCGGAGAAAGTAGACAGGCAAGTCAAGCTCCGAGACGCAAGGCACCTCCCATTGAAAATAAATTATCTTGCAGCCTTGAGGAGCTTTATAAAGGTACTACCAAAAAGATGAAGATCTCAAGGGAAATCGCATACGCAAGCGGTAAAACTCAACCTGTGGAGGAAATACTAACGATCGAAATCCAACCAGGCTGGAAAAAGGGAACAAAAATCACTTTCCCTGAGAAAGGAAACGAGCAGCCAAATGTTATTGCTGCAGATATTGTATTCATTATTGATGAGAAACCTCACAGTGTATTTACAAGAGAAGGCAATGATTTGGTTGTGACACAAAACATATCACTTGCAGAAGGTGAAGCTCTAACCAGCAGCTACACCTTCCAGCTTACTACATTGGATGGAAGAGATCTGACCATTGCCATAGACAATGGAATTGATCCAAACTATGAAGAAGTGGTTGCGGGCGAAGGGATGCCGATTTCCAAAAATCCTTCTCAGAGAGGCAGTCTTAGGATCAAATTCAACATCATATTGACAGACATGGTTGATGCTGAAACAGAAAGCCAGAACTAAGATATGCTCATAGTGCCATAATAAAGTGTCAACCATTGTAGTGTATCTTGTGTACAATATAATAATATTTCGTTTTGCTCCTCTTACCAAAGATATATGTAGCTTATGTGAGATCGTTTTACAGTGCCATCCATGCNANYAAAATCA
TARGET=139,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04743 Ps_contig_mira-and-tgicl-ass_franssen_34520
SEQUENCE=AAGCAAAGCAAGCACATGCAGATTGCAGACACACTTGCTTGGATAGATAAACCTCACTCACTCACTCAACCAGCGATTCCATTCCATTCCTTCTTTTCTTTTTCACACACAAGCACAAGCACAAACACATCATTCTTCACACAATTCATCAAACAAAACAAAAACCTCTTCACCTTTTTTGTCTCTCATGGAACGTTACACGCTACCCTTACCACAAGGACTTCATAATTCTCTTACTATACCTTGGTCTGAACCACAGCAACAACAACAATCACCTTCATGGTCCACACCTAACTCCGAACCTAAACTCAACAACAACAACAACAACGACCAAGACATTGCCATTGCTATTGCCATGGCTGCTTCTACTACCTCTCTCCCTTTCTTCAAACCCGAACCTGATAACTTCTACAACCTCAACTCTACTACTACAACAACCAACAACGTTGTTCCGTTTGTTTCAAATCCCAATTCTACAAATGATAATTTTCTCATGCACCAAAACACAAACACCATGGATTCAATTTCAAATCCACACCCTTTCTTCCACAACAACAACAACTACTTCTTCAACAATAGCAACAGCAATAATAACCCTTTTGAAATGGGATTCGACAATGGATTCTTTATGGGAAACAACAACAACAGCAACGCTTCCAATTCACCTGTTTTTATGGGTGCCTCCCTCGACCTGACTTCCGCTTCCGAGTTTCCACCGACTCTTGAACTCGATGCTTCTGTACCACCTTTCAGTGCTTCGTTTTCTATGCCTCTTGAGCTCTCTCAACCGCAACCTCAACAGCAGCAGCAGCAACAACAACAGCAGCCGACCACGCTGTTTCAGAAGCGGCGAGGGGCGTTGGAGATTCCGAGGTTGGAGACGGTGGGGAACAAGAAGAAGAGAAAGGTGGAGAAAAAATGGGAGGAAGATGGAAATGGTGGTGGTGAGGATGATGTTGAGGATTTCTCTGAGTTGAATTATGATTCTGATGAGAATGGAAATGATTTGAACAATTCCAATGGGACTGTTGTTACTGGTGGAGATCAAAAGGGGAAGAAGAAGAAGGGGCTTCCTGCCAAAAATCTCATGGCCGAGAGGCGTCGGAGGAAGAAACTCAATGATAGGTTGTACATGCTTAGGTCTGTTGTGCCAAAGATTAGCAAGATGGACAGGGCTTCAATACTTGGTGATGCAGTTGACTACTTGAAGGAGCTACTGCAGCGGATTAACAATCTTCATAACGAACTGGAATCAACTCCTCCTGGCTCATTGCTGCAACCTTCTGCAAGTGCAAGCTTTCACCCATTGACACCCACTCCACCGACTCTTCCTTGCCGAGTCAAGGAAGATCTATATCCTGGTGACTTGCTAAGCCCCAAAAACCAATCTCCTAAGGTGGAAGTTAGGGTAAGGGAAGGGAGAGCGGTCAACATTCATATGTTTTGTACCCGAAGACCAGGACTTTTGCTTTCTACCATGAGGGCTTTGGATAACCTTGGATTGGATGTTCAACAAGCTGTTATTAGTTGTTTCAATGGCTTTGCTTTGGATGTTTTCAGAGCCGAGCAGCAATGCAGAGAAGGTCAGGATGTTCCCCCCGAGCAAATTAAAGCAGTACTTTTGGATTCGGCAGGATATCATGGTTTGAATTGATTGAAGTGTCTGATAAAATGCTACCCTTATGGAGGTACTAACTGTAGCTGCTGTAGCTCCTTGTTTGTAGACGCATTGCTTCTTGTTCTTCGCCTTAGTAAAAACCCATGTTTCAGGACAAGATGAAATAGATTAGCTTAATCTTTAGGTGGAGAAGATAGCTGTTAACATTTTCTCAATTGCGCTTATGACAAATGCGACCGCAAGTTGTTCAATTTATGATTAAGTGGTGTAATGAATTGTTAATTTTTTTTTCCTRTCCATTACTCTATCAAGTTTTTTGCCTAT
TARGET=53,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04744 Ps_contig_mira-and-tgicl-ass_franssen_34520
SEQUENCE=AAGCAAAGCAAGCACATGCAGATTGCAGACACACTTGCTTGGATAGATAAACCTCACTCACTCACTCAACCAGCGATTCCATTCCATTCCTTCTTTTCTTTTTCACACACAAGCACAAGCACAAACACATCATTCTTCACACAATTCATCAAACAAAACAAAAACCTCTTCACCTTTTTTGTCTCTCATGGAACGTTACACGCTACCCTTACCACAAGGACTTCATAATTCTCTTACTATACCTTGGTCTGAACCACAGCAACAACAACAATCACCTTCATGGTCCACACCTAACTCCGAACCTAAACTCAACAACAACAACAACAACGACCAAGACATTGCCATTGCTATTGCCATGGCTGCTTCTACTACCTCTCTCCCTTTCTTCAAACCCGAACCTGATAACTTCTACAACCTCAACTCTACTACTACAACAACCAACAACGTTGTTCCGTTTGTTTCAAATCCCAATTCTACAAATGATAATTTTCTCATGCACCAAAACACAAACACCATGGATTCAATTTCAAATCCACACCCTTTCTTCCACAACAACAACAACTACTTCTTCAACAATAGCAACAGCAATAATAACCCTTTTGAAATGGGATTCGACAATGGATTCTTTATGGGAAACAACAACAACAGCAACGCTTCCAATTCACCTGTTTTTATGGGTGCCTCCCTCGACCTGACTTCCGCTTCCGAGTTTCCACCGACTCTTGAACTCGATGCTTCTGTACCACCTTTCAGTGCTTCGTTTTCTATGCCTCTTGAGCTCTCTCAACCGCAACCTCAACAGCAGCAGCAGCAACAACAACAGCAGCCGACCACGCTGTTTCAGAAGCGGCGAGGGGCGTTGGAGATTCCGAGGTTGGAGACGGTGGGGAACAAGAAGAAGAGAAAGGTGGAGAAAAAATGGGAGGAAGATGGAAATGGTGGTGGTGAGGATGATGTTGAGGATTTCTCTGAGTTGAATTATGATTCTGATGAGAATGGAAATGATTTGAACAATTCCAATGGGACTGTTGTTACTGGTGGAGATCAAAAGGGGAAGAAGAAGAAGGGGCTTCCTGCCAAAAATCTCATGGCCGAGAGGCGTCGGAGGAAGAAACTCAATGATAGGTTGTACATGCTTAGGTCTGTTGTGCCAAAGATTAGCAAGATGGACAGGGCTTCAATACTTGGTGATGCAGTTGACTACTTGAAGGAGCTACTGCAGCGGATTAACAATCTTCATAACGAACTGGAATCAACTCCTCCTGGCTCATTGCTGCAACCTTCTGCAAGTGCAAGCTTTCACCCATTGACACCCACTCCACCGACTCTTCCTTGCCGAGTCAAGGAAGATCTATATCCTGGTGACTTGCTAAGCCCCAAAAACCAATCTCCTAAGGTGGAAGTTAGGGTAAGGGAAGGGAGAGCGGTCAACATTCATATGTTTTGTACCCGAAGACCAGGACTTTTGCTTTCTACCATGAGGGCTTTGGATAACCTTGGATTGGATGTTCAACAAGCTGTTATTAGTTGTTTCAATGGCTTTGCTTTGGATGTTTTCAGAGCCGAGCAGCAATGCAGAGAAGGTCAGGATGTTCCCCCCGAGCAAATTAAAGCAGTACTTTTGGATTCGGCAGGATATCATGGTTTGAATTGATTGAAGTGTCTGATAAAATGCTACCCTTATGGAGGTACTAACTGTAGCTGCTGTAGCTCCTTGTTTGTAGACGCATTGCTTCTTGTTCTTCGCCTTAGTAAAAACCCATGTTTCAGGACAAGATGAAATAGATTAGCTTAATCTTTAGGTGGAGAAGATAGCTGTTAACATTTTCTCAATTGCGCTTATGACAAATGCGACCGCAAGTTGTTCAATTTATGATTAAGTGGTGTAATGAATTGTTAATTTTTTTTTCCTRTCCATTACTCTATCAAGTTTTTTGCCTAT
TARGET=76,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04745 Ps_contig_mira-and-tgicl-ass_franssen_34520
SEQUENCE=AAGCAAAGCAAGCACATGCAGATTGCAGACACACTTGCTTGGATAGATAAACCTCACTCACTCACTCAACCAGCGATTCCATTCCATTCCTTCTTTTCTTTTTCACACACAAGCACAAGCACAAACACATCATTCTTCACACAATTCATCAAACAAAACAAAAACCTCTTCACCTTTTTTGTCTCTCATGGAACGTTACACGCTACCCTTACCACAAGGACTTCATAATTCTCTTACTATACCTTGGTCTGAACCACAGCAACAACAACAATCACCTTCATGGTCCACACCTAACTCCGAACCTAAACTCAACAACAACAACAACAACGACCAAGACATTGCCATTGCTATTGCCATGGCTGCTTCTACTACCTCTCTCCCTTTCTTCAAACCCGAACCTGATAACTTCTACAACCTCAACTCTACTACTACAACAACCAACAACGTTGTTCCGTTTGTTTCAAATCCCAATTCTACAAATGATAATTTTCTCATGCACCAAAACACAAACACCATGGATTCAATTTCAAATCCACACCCTTTCTTCCACAACAACAACAACTACTTCTTCAACAATAGCAACAGCAATAATAACCCTTTTGAAATGGGATTCGACAATGGATTCTTTATGGGAAACAACAACAACAGCAACGCTTCCAATTCACCTGTTTTTATGGGTGCCTCCCTCGACCTGACTTCCGCTTCCGAGTTTCCACCGACTCTTGAACTCGATGCTTCTGTACCACCTTTCAGTGCTTCGTTTTCTATGCCTCTTGAGCTCTCTCAACCGCAACCTCAACAGCAGCAGCAGCAACAACAACAGCAGCCGACCACGCTGTTTCAGAAGCGGCGAGGGGCGTTGGAGATTCCGAGGTTGGAGACGGTGGGGAACAAGAAGAAGAGAAAGGTGGAGAAAAAATGGGAGGAAGATGGAAATGGTGGTGGTGAGGATGATGTTGAGGATTTCTCTGAGTTGAATTATGATTCTGATGAGAATGGAAATGATTTGAACAATTCCAATGGGACTGTTGTTACTGGTGGAGATCAAAAGGGGAAGAAGAAGAAGGGGCTTCCTGCCAAAAATCTCATGGCCGAGAGGCGTCGGAGGAAGAAACTCAATGATAGGTTGTACATGCTTAGGTCTGTTGTGCCAAAGATTAGCAAGATGGACAGGGCTTCAATACTTGGTGATGCAGTTGACTACTTGAAGGAGCTACTGCAGCGGATTAACAATCTTCATAACGAACTGGAATCAACTCCTCCTGGCTCATTGCTGCAACCTTCTGCAAGTGCAAGCTTTCACCCATTGACACCCACTCCACCGACTCTTCCTTGCCGAGTCAAGGAAGATCTATATCCTGGTGACTTGCTAAGCCCCAAAAACCAATCTCCTAAGGTGGAAGTTAGGGTAAGGGAAGGGAGAGCGGTCAACATTCATATGTTTTGTACCCGAAGACCAGGACTTTTGCTTTCTACCATGAGGGCTTTGGATAACCTTGGATTGGATGTTCAACAAGCTGTTATTAGTTGTTTCAATGGCTTTGCTTTGGATGTTTTCAGAGCCGAGCAGCAATGCAGAGAAGGTCAGGATGTTCCCCCCGAGCAAATTAAAGCAGTACTTTTGGATTCGGCAGGATATCATGGTTTGAATTGATTGAAGTGTCTGATAAAATGCTACCCTTATGGAGGTACTAACTGTAGCTGCTGTAGCTCCTTGTTTGTAGACGCATTGCTTCTTGTTCTTCGCCTTAGTAAAAACCCATGTTTCAGGACAAGATGAAATAGATTAGCTTAATCTTTAGGTGGAGAAGATAGCTGTTAACATTTTCTCAATTGCGCTTATGACAAATGCGACCGCAAGTTGTTCAATTTATGATTAAGTGGTGTAATGAATTGTTAATTTTTTTTTCCTRTCCATTACTCTATCAAGTTTTTTGCCTAT
TARGET=260,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04746 Ps_contig_mira-and-tgicl-ass_franssen_34520
SEQUENCE=AAGCAAAGCAAGCACATGCAGATTGCAGACACACTTGCTTGGATAGATAAACCTCACTCACTCACTCAACCAGCGATTCCATTCCATTCCTTCTTTTCTTTTTCACACACAAGCACAAGCACAAACACATCATTCTTCACACAATTCATCAAACAAAACAAAAACCTCTTCACCTTTTTTGTCTCTCATGGAACGTTACACGCTACCCTTACCACAAGGACTTCATAATTCTCTTACTATACCTTGGTCTGAACCACAGCAACAACAACAATCACCTTCATGGTCCACACCTAACTCCGAACCTAAACTCAACAACAACAACAACAACGACCAAGACATTGCCATTGCTATTGCCATGGCTGCTTCTACTACCTCTCTCCCTTTCTTCAAACCCGAACCTGATAACTTCTACAACCTCAACTCTACTACTACAACAACCAACAACGTTGTTCCGTTTGTTTCAAATCCCAATTCTACAAATGATAATTTTCTCATGCACCAAAACACAAACACCATGGATTCAATTTCAAATCCACACCCTTTCTTCCACAACAACAACAACTACTTCTTCAACAATAGCAACAGCAATAATAACCCTTTTGAAATGGGATTCGACAATGGATTCTTTATGGGAAACAACAACAACAGCAACGCTTCCAATTCACCTGTTTTTATGGGTGCCTCCCTCGACCTGACTTCCGCTTCCGAGTTTCCACCGACTCTTGAACTCGATGCTTCTGTACCACCTTTCAGTGCTTCGTTTTCTATGCCTCTTGAGCTCTCTCAACCGCAACCTCAACAGCAGCAGCAGCAACAACAACAGCAGCCGACCACGCTGTTTCAGAAGCGGCGAGGGGCGTTGGAGATTCCGAGGTTGGAGACGGTGGGGAACAAGAAGAAGAGAAAGGTGGAGAAAAAATGGGAGGAAGATGGAAATGGTGGTGGTGAGGATGATGTTGAGGATTTCTCTGAGTTGAATTATGATTCTGATGAGAATGGAAATGATTTGAACAATTCCAATGGGACTGTTGTTACTGGTGGAGATCAAAAGGGGAAGAAGAAGAAGGGGCTTCCTGCCAAAAATCTCATGGCCGAGAGGCGTCGGAGGAAGAAACTCAATGATAGGTTGTACATGCTTAGGTCTGTTGTGCCAAAGATTAGCAAGATGGACAGGGCTTCAATACTTGGTGATGCAGTTGACTACTTGAAGGAGCTACTGCAGCGGATTAACAATCTTCATAACGAACTGGAATCAACTCCTCCTGGCTCATTGCTGCAACCTTCTGCAAGTGCAAGCTTTCACCCATTGACACCCACTCCACCGACTCTTCCTTGCCGAGTCAAGGAAGATCTATATCCTGGTGACTTGCTAAGCCCCAAAAACCAATCTCCTAAGGTGGAAGTTAGGGTAAGGGAAGGGAGAGCGGTCAACATTCATATGTTTTGTACCCGAAGACCAGGACTTTTGCTTTCTACCATGAGGGCTTTGGATAACCTTGGATTGGATGTTCAACAAGCTGTTATTAGTTGTTTCAATGGCTTTGCTTTGGATGTTTTCAGAGCCGAGCAGCAATGCAGAGAAGGTCAGGATGTTCCCCCCGAGCAAATTAAAGCAGTACTTTTGGATTCGGCAGGATATCATGGTTTGAATTGATTGAAGTGTCTGATAAAATGCTACCCTTATGGAGGTACTAACTGTAGCTGCTGTAGCTCCTTGTTTGTAGACGCATTGCTTCTTGTTCTTCGCCTTAGTAAAAACCCATGTTTCAGGACAAGATGAAATAGATTAGCTTAATCTTTAGGTGGAGAAGATAGCTGTTAACATTTTCTCAATTGCGCTTATGACAAATGCGACCGCAAGTTGTTCAATTTATGATTAAGTGGTGTAATGAATTGTTAATTTTTTTTTCCTRTCCATTACTCTATCAAGTTTTTTGCCTAT
TARGET=310,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04747 Ps_contig_mira-and-tgicl-ass_franssen_34520
SEQUENCE=AAGCAAAGCAAGCACATGCAGATTGCAGACACACTTGCTTGGATAGATAAACCTCACTCACTCACTCAACCAGCGATTCCATTCCATTCCTTCTTTTCTTTTTCACACACAAGCACAAGCACAAACACATCATTCTTCACACAATTCATCAAACAAAACAAAAACCTCTTCACCTTTTTTGTCTCTCATGGAACGTTACACGCTACCCTTACCACAAGGACTTCATAATTCTCTTACTATACCTTGGTCTGAACCACAGCAACAACAACAATCACCTTCATGGTCCACACCTAACTCCGAACCTAAACTCAACAACAACAACAACAACGACCAAGACATTGCCATTGCTATTGCCATGGCTGCTTCTACTACCTCTCTCCCTTTCTTCAAACCCGAACCTGATAACTTCTACAACCTCAACTCTACTACTACAACAACCAACAACGTTGTTCCGTTTGTTTCAAATCCCAATTCTACAAATGATAATTTTCTCATGCACCAAAACACAAACACCATGGATTCAATTTCAAATCCACACCCTTTCTTCCACAACAACAACAACTACTTCTTCAACAATAGCAACAGCAATAATAACCCTTTTGAAATGGGATTCGACAATGGATTCTTTATGGGAAACAACAACAACAGCAACGCTTCCAATTCACCTGTTTTTATGGGTGCCTCCCTCGACCTGACTTCCGCTTCCGAGTTTCCACCGACTCTTGAACTCGATGCTTCTGTACCACCTTTCAGTGCTTCGTTTTCTATGCCTCTTGAGCTCTCTCAACCGCAACCTCAACAGCAGCAGCAGCAACAACAACAGCAGCCGACCACGCTGTTTCAGAAGCGGCGAGGGGCGTTGGAGATTCCGAGGTTGGAGACGGTGGGGAACAAGAAGAAGAGAAAGGTGGAGAAAAAATGGGAGGAAGATGGAAATGGTGGTGGTGAGGATGATGTTGAGGATTTCTCTGAGTTGAATTATGATTCTGATGAGAATGGAAATGATTTGAACAATTCCAATGGGACTGTTGTTACTGGTGGAGATCAAAAGGGGAAGAAGAAGAAGGGGCTTCCTGCCAAAAATCTCATGGCCGAGAGGCGTCGGAGGAAGAAACTCAATGATAGGTTGTACATGCTTAGGTCTGTTGTGCCAAAGATTAGCAAGATGGACAGGGCTTCAATACTTGGTGATGCAGTTGACTACTTGAAGGAGCTACTGCAGCGGATTAACAATCTTCATAACGAACTGGAATCAACTCCTCCTGGCTCATTGCTGCAACCTTCTGCAAGTGCAAGCTTTCACCCATTGACACCCACTCCACCGACTCTTCCTTGCCGAGTCAAGGAAGATCTATATCCTGGTGACTTGCTAAGCCCCAAAAACCAATCTCCTAAGGTGGAAGTTAGGGTAAGGGAAGGGAGAGCGGTCAACATTCATATGTTTTGTACCCGAAGACCAGGACTTTTGCTTTCTACCATGAGGGCTTTGGATAACCTTGGATTGGATGTTCAACAAGCTGTTATTAGTTGTTTCAATGGCTTTGCTTTGGATGTTTTCAGAGCCGAGCAGCAATGCAGAGAAGGTCAGGATGTTCCCCCCGAGCAAATTAAAGCAGTACTTTTGGATTCGGCAGGATATCATGGTTTGAATTGATTGAAGTGTCTGATAAAATGCTACCCTTATGGAGGTACTAACTGTAGCTGCTGTAGCTCCTTGTTTGTAGACGCATTGCTTCTTGTTCTTCGCCTTAGTAAAAACCCATGTTTCAGGACAAGATGAAATAGATTAGCTTAATCTTTAGGTGGAGAAGATAGCTGTTAACATTTTCTCAATTGCGCTTATGACAAATGCGACCGCAAGTTGTTCAATTTATGATTAAGTGGTGTAATGAATTGTTAATTTTTTTTTCCTRTCCATTACTCTATCAAGTTTTTTGCCTAT
TARGET=549,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04748 Ps_contig_mira-and-tgicl-ass_franssen_34520
SEQUENCE=AAGCAAAGCAAGCACATGCAGATTGCAGACACACTTGCTTGGATAGATAAACCTCACTCACTCACTCAACCAGCGATTCCATTCCATTCCTTCTTTTCTTTTTCACACACAAGCACAAGCACAAACACATCATTCTTCACACAATTCATCAAACAAAACAAAAACCTCTTCACCTTTTTTGTCTCTCATGGAACGTTACACGCTACCCTTACCACAAGGACTTCATAATTCTCTTACTATACCTTGGTCTGAACCACAGCAACAACAACAATCACCTTCATGGTCCACACCTAACTCCGAACCTAAACTCAACAACAACAACAACAACGACCAAGACATTGCCATTGCTATTGCCATGGCTGCTTCTACTACCTCTCTCCCTTTCTTCAAACCCGAACCTGATAACTTCTACAACCTCAACTCTACTACTACAACAACCAACAACGTTGTTCCGTTTGTTTCAAATCCCAATTCTACAAATGATAATTTTCTCATGCACCAAAACACAAACACCATGGATTCAATTTCAAATCCACACCCTTTCTTCCACAACAACAACAACTACTTCTTCAACAATAGCAACAGCAATAATAACCCTTTTGAAATGGGATTCGACAATGGATTCTTTATGGGAAACAACAACAACAGCAACGCTTCCAATTCACCTGTTTTTATGGGTGCCTCCCTCGACCTGACTTCCGCTTCCGAGTTTCCACCGACTCTTGAACTCGATGCTTCTGTACCACCTTTCAGTGCTTCGTTTTCTATGCCTCTTGAGCTCTCTCAACCGCAACCTCAACAGCAGCAGCAGCAACAACAACAGCAGCCGACCACGCTGTTTCAGAAGCGGCGAGGGGCGTTGGAGATTCCGAGGTTGGAGACGGTGGGGAACAAGAAGAAGAGAAAGGTGGAGAAAAAATGGGAGGAAGATGGAAATGGTGGTGGTGAGGATGATGTTGAGGATTTCTCTGAGTTGAATTATGATTCTGATGAGAATGGAAATGATTTGAACAATTCCAATGGGACTGTTGTTACTGGTGGAGATCAAAAGGGGAAGAAGAAGAAGGGGCTTCCTGCCAAAAATCTCATGGCCGAGAGGCGTCGGAGGAAGAAACTCAATGATAGGTTGTACATGCTTAGGTCTGTTGTGCCAAAGATTAGCAAGATGGACAGGGCTTCAATACTTGGTGATGCAGTTGACTACTTGAAGGAGCTACTGCAGCGGATTAACAATCTTCATAACGAACTGGAATCAACTCCTCCTGGCTCATTGCTGCAACCTTCTGCAAGTGCAAGCTTTCACCCATTGACACCCACTCCACCGACTCTTCCTTGCCGAGTCAAGGAAGATCTATATCCTGGTGACTTGCTAAGCCCCAAAAACCAATCTCCTAAGGTGGAAGTTAGGGTAAGGGAAGGGAGAGCGGTCAACATTCATATGTTTTGTACCCGAAGACCAGGACTTTTGCTTTCTACCATGAGGGCTTTGGATAACCTTGGATTGGATGTTCAACAAGCTGTTATTAGTTGTTTCAATGGCTTTGCTTTGGATGTTTTCAGAGCCGAGCAGCAATGCAGAGAAGGTCAGGATGTTCCCCCCGAGCAAATTAAAGCAGTACTTTTGGATTCGGCAGGATATCATGGTTTGAATTGATTGAAGTGTCTGATAAAATGCTACCCTTATGGAGGTACTAACTGTAGCTGCTGTAGCTCCTTGTTTGTAGACGCATTGCTTCTTGTTCTTCGCCTTAGTAAAAACCCATGTTTCAGGACAAGATGAAATAGATTAGCTTAATCTTTAGGTGGAGAAGATAGCTGTTAACATTTTCTCAATTGCGCTTATGACAAATGCGACCGCAAGTTGTTCAATTTATGATTAAGTGGTGTAATGAATTGTTAATTTTTTTTTCCTRTCCATTACTCTATCAAGTTTTTTGCCTAT
TARGET=635,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04749 Ps_contig_mira-and-tgicl-ass_franssen_34520
SEQUENCE=AAGCAAAGCAAGCACATGCAGATTGCAGACACACTTGCTTGGATAGATAAACCTCACTCACTCACTCAACCAGCGATTCCATTCCATTCCTTCTTTTCTTTTTCACACACAAGCACAAGCACAAACACATCATTCTTCACACAATTCATCAAACAAAACAAAAACCTCTTCACCTTTTTTGTCTCTCATGGAACGTTACACGCTACCCTTACCACAAGGACTTCATAATTCTCTTACTATACCTTGGTCTGAACCACAGCAACAACAACAATCACCTTCATGGTCCACACCTAACTCCGAACCTAAACTCAACAACAACAACAACAACGACCAAGACATTGCCATTGCTATTGCCATGGCTGCTTCTACTACCTCTCTCCCTTTCTTCAAACCCGAACCTGATAACTTCTACAACCTCAACTCTACTACTACAACAACCAACAACGTTGTTCCGTTTGTTTCAAATCCCAATTCTACAAATGATAATTTTCTCATGCACCAAAACACAAACACCATGGATTCAATTTCAAATCCACACCCTTTCTTCCACAACAACAACAACTACTTCTTCAACAATAGCAACAGCAATAATAACCCTTTTGAAATGGGATTCGACAATGGATTCTTTATGGGAAACAACAACAACAGCAACGCTTCCAATTCACCTGTTTTTATGGGTGCCTCCCTCGACCTGACTTCCGCTTCCGAGTTTCCACCGACTCTTGAACTCGATGCTTCTGTACCACCTTTCAGTGCTTCGTTTTCTATGCCTCTTGAGCTCTCTCAACCGCAACCTCAACAGCAGCAGCAGCAACAACAACAGCAGCCGACCACGCTGTTTCAGAAGCGGCGAGGGGCGTTGGAGATTCCGAGGTTGGAGACGGTGGGGAACAAGAAGAAGAGAAAGGTGGAGAAAAAATGGGAGGAAGATGGAAATGGTGGTGGTGAGGATGATGTTGAGGATTTCTCTGAGTTGAATTATGATTCTGATGAGAATGGAAATGATTTGAACAATTCCAATGGGACTGTTGTTACTGGTGGAGATCAAAAGGGGAAGAAGAAGAAGGGGCTTCCTGCCAAAAATCTCATGGCCGAGAGGCGTCGGAGGAAGAAACTCAATGATAGGTTGTACATGCTTAGGTCTGTTGTGCCAAAGATTAGCAAGATGGACAGGGCTTCAATACTTGGTGATGCAGTTGACTACTTGAAGGAGCTACTGCAGCGGATTAACAATCTTCATAACGAACTGGAATCAACTCCTCCTGGCTCATTGCTGCAACCTTCTGCAAGTGCAAGCTTTCACCCATTGACACCCACTCCACCGACTCTTCCTTGCCGAGTCAAGGAAGATCTATATCCTGGTGACTTGCTAAGCCCCAAAAACCAATCTCCTAAGGTGGAAGTTAGGGTAAGGGAAGGGAGAGCGGTCAACATTCATATGTTTTGTACCCGAAGACCAGGACTTTTGCTTTCTACCATGAGGGCTTTGGATAACCTTGGATTGGATGTTCAACAAGCTGTTATTAGTTGTTTCAATGGCTTTGCTTTGGATGTTTTCAGAGCCGAGCAGCAATGCAGAGAAGGTCAGGATGTTCCCCCCGAGCAAATTAAAGCAGTACTTTTGGATTCGGCAGGATATCATGGTTTGAATTGATTGAAGTGTCTGATAAAATGCTACCCTTATGGAGGTACTAACTGTAGCTGCTGTAGCTCCTTGTTTGTAGACGCATTGCTTCTTGTTCTTCGCCTTAGTAAAAACCCATGTTTCAGGACAAGATGAAATAGATTAGCTTAATCTTTAGGTGGAGAAGATAGCTGTTAACATTTTCTCAATTGCGCTTATGACAAATGCGACCGCAAGTTGTTCAATTTATGATTAAGTGGTGTAATGAATTGTTAATTTTTTTTTCCTRTCCATTACTCTATCAAGTTTTTTGCCTAT
TARGET=800,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04750 Ps_contig_mira-and-tgicl-ass_franssen_34520
SEQUENCE=AAGCAAAGCAAGCACATGCAGATTGCAGACACACTTGCTTGGATAGATAAACCTCACTCACTCACTCAACCAGCGATTCCATTCCATTCCTTCTTTTCTTTTTCACACACAAGCACAAGCACAAACACATCATTCTTCACACAATTCATCAAACAAAACAAAAACCTCTTCACCTTTTTTGTCTCTCATGGAACGTTACACGCTACCCTTACCACAAGGACTTCATAATTCTCTTACTATACCTTGGTCTGAACCACAGCAACAACAACAATCACCTTCATGGTCCACACCTAACTCCGAACCTAAACTCAACAACAACAACAACAACGACCAAGACATTGCCATTGCTATTGCCATGGCTGCTTCTACTACCTCTCTCCCTTTCTTCAAACCCGAACCTGATAACTTCTACAACCTCAACTCTACTACTACAACAACCAACAACGTTGTTCCGTTTGTTTCAAATCCCAATTCTACAAATGATAATTTTCTCATGCACCAAAACACAAACACCATGGATTCAATTTCAAATCCACACCCTTTCTTCCACAACAACAACAACTACTTCTTCAACAATAGCAACAGCAATAATAACCCTTTTGAAATGGGATTCGACAATGGATTCTTTATGGGAAACAACAACAACAGCAACGCTTCCAATTCACCTGTTTTTATGGGTGCCTCCCTCGACCTGACTTCCGCTTCCGAGTTTCCACCGACTCTTGAACTCGATGCTTCTGTACCACCTTTCAGTGCTTCGTTTTCTATGCCTCTTGAGCTCTCTCAACCGCAACCTCAACAGCAGCAGCAGCAACAACAACAGCAGCCGACCACGCTGTTTCAGAAGCGGCGAGGGGCGTTGGAGATTCCGAGGTTGGAGACGGTGGGGAACAAGAAGAAGAGAAAGGTGGAGAAAAAATGGGAGGAAGATGGAAATGGTGGTGGTGAGGATGATGTTGAGGATTTCTCTGAGTTGAATTATGATTCTGATGAGAATGGAAATGATTTGAACAATTCCAATGGGACTGTTGTTACTGGTGGAGATCAAAAGGGGAAGAAGAAGAAGGGGCTTCCTGCCAAAAATCTCATGGCCGAGAGGCGTCGGAGGAAGAAACTCAATGATAGGTTGTACATGCTTAGGTCTGTTGTGCCAAAGATTAGCAAGATGGACAGGGCTTCAATACTTGGTGATGCAGTTGACTACTTGAAGGAGCTACTGCAGCGGATTAACAATCTTCATAACGAACTGGAATCAACTCCTCCTGGCTCATTGCTGCAACCTTCTGCAAGTGCAAGCTTTCACCCATTGACACCCACTCCACCGACTCTTCCTTGCCGAGTCAAGGAAGATCTATATCCTGGTGACTTGCTAAGCCCCAAAAACCAATCTCCTAAGGTGGAAGTTAGGGTAAGGGAAGGGAGAGCGGTCAACATTCATATGTTTTGTACCCGAAGACCAGGACTTTTGCTTTCTACCATGAGGGCTTTGGATAACCTTGGATTGGATGTTCAACAAGCTGTTATTAGTTGTTTCAATGGCTTTGCTTTGGATGTTTTCAGAGCCGAGCAGCAATGCAGAGAAGGTCAGGATGTTCCCCCCGAGCAAATTAAAGCAGTACTTTTGGATTCGGCAGGATATCATGGTTTGAATTGATTGAAGTGTCTGATAAAATGCTACCCTTATGGAGGTACTAACTGTAGCTGCTGTAGCTCCTTGTTTGTAGACGCATTGCTTCTTGTTCTTCGCCTTAGTAAAAACCCATGTTTCAGGACAAGATGAAATAGATTAGCTTAATCTTTAGGTGGAGAAGATAGCTGTTAACATTTTCTCAATTGCGCTTATGACAAATGCGACCGCAAGTTGTTCAATTTATGATTAAGTGGTGTAATGAATTGTTAATTTTTTTTTCCTRTCCATTACTCTATCAAGTTTTTTGCCTAT
TARGET=1054,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04751 Ps_contig_mira-and-tgicl-ass_franssen_34521
SEQUENCE=AACATCTAATCACAAAATTTAAAATATAAAATAAAACCATTCTACACAAGAATGATGAACAAATTAGAAGAAAAAAACAAATCTGTACATAAACCTAAAAAGAAAAAAGAATCACAAATTTCTAAACAACAACAACAACAAAAGTANCNAAAAGATTCATACTAAAGGATTTCGCGATTTCGGTCTGGTCTGGCCATCTGTTCATACAGGTGTTGCGAAGCTGATTGCAATCGTTGATCGTTGCCTCGGCGTGAGGCGAGGCGAATAGGTGAATACACTACACTATAGGTATCGCACCTTCCAATACCGTTCTGTCGCGCCTGTTTTCGCTTGATCACAACAAATCATGAGCATATCCAACCAAAATCGAAGACGAAAAACTCCAAGGTTGAACCTCTTGATCTTTATAATTCCTGGGCTTATAAAGATCATAAGGCTCCCAAAGTGAATCCAAAGGACAAGCTTTCTTCTCATCAAGCCTTACCCAGGGCTTCCCTTTCCCACTCCAATGCAACAAACTCACAGGACCAGGATGCAAAGATCTACAAACCCCATTCAAATTATCACCCCCAAGTCCATGTTGATTCCATCTATGATCAATAGCTTCAACGTTTCCTGCAAAAACAAGCAAAAACGGTGGCAATGAACCCAGTTCATAAATCCTCTTCTTCTTCTGCAACTCCATCCAATTCTCAATCCTCTTCCTATAATTCCCTTCCCTCCATTTCACCATATCCATAACCATCACACCCGTGTTGAAATAACAAGCTTTTCTCGTTCCAAACACACGAGACAACAAAGGGTCAACCCAGAATTCGTCTGTGAAATACTTTGTGAAATTCGCATGGCAATATTCTGGTGCACCAATAACCTTCGACTCACCCATTTTCACACTCCATAGCTTGAAGATATCATCAACAACGACAATATCCGAGTCAAGATAGATAACTCGTTCCACACACGAGTCAAGCATGTCACCCAAATAGTTTCTCGCGTAGTTCAATGGATTCTCCAGCGCTAAACGAATGGAGGAGGAGATAAGGTTTATCACAGTGTCTTCACGAAAGATGTAAACTTTGAAGTTTAGCGACGGGAAAATGGATCTTACCAAACGAGTCAAGAGACGTGGACTCACTGGGTCAAACTCGGCGGAGATGAAATGGAAGTAAAACGTTTTTCGTGGGGCAGGGAGGGAGG
TARGET=125,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04752 Ps_contig_mira-and-tgicl-ass_franssen_34521
SEQUENCE=AACATCTAATCACAAAATTTAAAATATAAAATAAAACCATTCTACACAAGAATGATGAACAAATTAGAAGAAAAAAACAAATCTGTACATAAACCTAAAAAGAAAAAAGAATCACAAATTTCTAAACAACAACAACAACAAAAGTANCNAAAAGATTCATACTAAAGGATTTCGCGATTTCGGTCTGGTCTGGCCATCTGTTCATACAGGTGTTGCGAAGCTGATTGCAATCGTTGATCGTTGCCTCGGCGTGAGGCGAGGCGAATAGGTGAATACACTACACTATAGGTATCGCACCTTCCAATACCGTTCTGTCGCGCCTGTTTTCGCTTGATCACAACAAATCATGAGCATATCCAACCAAAATCGAAGACGAAAAACTCCAAGGTTGAACCTCTTGATCTTTATAATTCCTGGGCTTATAAAGATCATAAGGCTCCCAAAGTGAATCCAAAGGACAAGCTTTCTTCTCATCAAGCCTTACCCAGGGCTTCCCTTTCCCACTCCAATGCAACAAACTCACAGGACCAGGATGCAAAGATCTACAAACCCCATTCAAATTATCACCCCCAAGTCCATGTTGATTCCATCTATGATCAATAGCTTCAACGTTTCCTGCAAAAACAAGCAAAAACGGTGGCAATGAACCCAGTTCATAAATCCTCTTCTTCTTCTGCAACTCCATCCAATTCTCAATCCTCTTCCTATAATTCCCTTCCCTCCATTTCACCATATCCATAACCATCACACCCGTGTTGAAATAACAAGCTTTTCTCGTTCCAAACACACGAGACAACAAAGGGTCAACCCAGAATTCGTCTGTGAAATACTTTGTGAAATTCGCATGGCAATATTCTGGTGCACCAATAACCTTCGACTCACCCATTTTCACACTCCATAGCTTGAAGATATCATCAACAACGACAATATCCGAGTCAAGATAGATAACTCGTTCCACACACGAGTCAAGCATGTCACCCAAATAGTTTCTCGCGTAGTTCAATGGATTCTCCAGCGCTAAACGAATGGAGGAGGAGATAAGGTTTATCACAGTGTCTTCACGAAAGATGTAAACTTTGAAGTTTAGCGACGGGAAAATGGATCTTACCAAACGAGTCAAGAGACGTGGACTCACTGGGTCAAACTCGGCGGAGATGAAATGGAAGTAAAACGTTTTTCGTGGGGCAGGGAGGGAGG
TARGET=664,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1286
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.096820
PRIMER_LEFT_SEQUENCE=GGACCAGGATGCAAAGATCTAC
PRIMER_RIGHT_SEQUENCE=AGAGGATTGAGAATTGGATGGA
PRIMER_LEFT=524,22
PRIMER_RIGHT=701,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=59.903
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.1286
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.096820
PRIMER_LEFT_1_SEQUENCE=GGACCAGGATGCAAAGATCTAC
PRIMER_RIGHT_1_SEQUENCE=GAGGATTGAGAATTGGATGGAG
PRIMER_LEFT_1=524,22
PRIMER_RIGHT_1=700,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=59.903
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.1309
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.096820
PRIMER_LEFT_2_SEQUENCE=ATCATAAGGCTCCCAAAGTGAA
PRIMER_RIGHT_2_SEQUENCE=GAGGATTGAGAATTGGATGGAG
PRIMER_LEFT_2=427,22
PRIMER_RIGHT_2=700,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.903
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=0.1309
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.096820
PRIMER_LEFT_3_SEQUENCE=ATCATAAGGCTCCCAAAGTGAA
PRIMER_RIGHT_3_SEQUENCE=AGAGGATTGAGAATTGGATGGA
PRIMER_LEFT_3=427,22
PRIMER_RIGHT_3=701,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.903
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=0.1309
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.096820
PRIMER_LEFT_4_SEQUENCE=TCATAAGGCTCCCAAAGTGAAT
PRIMER_RIGHT_4_SEQUENCE=GAGGATTGAGAATTGGATGGAG
PRIMER_LEFT_4=428,22
PRIMER_RIGHT_4=700,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.903
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_04753 Ps_contig_mira-and-tgicl-ass_franssen_34523
SEQUENCE=GACACAAGAGAGAGAAAAGCAAAAGAATAACATAGTTGACACTGATACATAAATTTTGGACTTTGTTTCTATTTGGCACCCACCCACTCACACCTACAACAACACAAACAAAACAAAAACACAACATAGTAACAAGAAGCAGAAGAAGAAAGAGGAACAACAACAACAACAACAACAACAGAGATCTAACTGAGATCGAGCTTCAACTCTTTCTTACTCTATTTTTTCACACTCTTTGCTGTTTCAGTGTCATGGGGAGAGAAGTTACAGCAATGGAAGTTGAGGAGAATAAGCCAAATGGTGTAACAACAGATTCAAATGAGAGTGCTAATGATAATGGACACGATTCTTCCAAAATCGCGGATCCTGAAGTAAAGAAATGTGCCGAAGTAGATTTGTTTGTTGAGGATTGCCGCGAAAGTAAGGACATTTTGACAAACTGTAATGCCGATTTGCCGATAGAGGAGAATGAGAAACATGAAGTGCAGAAAACGGGTGACGAGAAAGAGTTAAGCTCGACAAAAGCATCGGATCCGGTTACTGAAAAGAATGGATCCTACACGCGTGTTGTTGATACTACTGAAGCTGTTGTAACTCGTTTGAACTTGTCGCCTAATGCTAACAACATGCACTCGTCGCCTTACTCGTCAAAGAATTCGCAGCAAAACTCGCCTTTTTCTTCAGCTAAGCCTTTGCAAAATGGTGATAAGAAGAACTACGATGATGAAGATAATTGGTCGGTTGCTTCCTCTGCTGTGTCAATGCGCACTGCGAGATCAAAGGTAACTCAGGGTTCAGCCCCTACATTTCGAAGCTCAGAACGTGCGGAAAARCGGAGAGAGTTTTATCTCAAATTAGAGGAGAAAAACCGAGCTCTTCTAGAAGAAAAAAGCCAGTATGAGGCAAGACAAAAGGAAGAGCAAGAAGCAGCAATCAAGCAGATGAGAAAGAACTTGGTAATTAGAGCAAAACCGGTACCGAGTTTCTACTATGAGGCGCCTCCGCCTAAAACTGAACTCAAGAAGCTGCCACTAACACGCCCAAAGTCACCGAAACTGAATATGAATCGTAGAAGAACCTTTGGCGATGCTGTGAACTCACAGCCTCCTGAAGTTTGTAGCCGAGCACGTCACAGCACTGGTAGCCACCACATCAAGAGCGGTTATAACACTAACCCTCTCACTGAAAAAACTAAGGACCAAGCCACGCGACGCCATAGCAATGGCGCTTACAAAACCAAAGAAAGACCAAAAGTGGATACAGAAACAAAAACTGCTCCTCCAAACATCATCCAGCATCCGAATGCAGACATATCAGTCCAATCATGATGACTCGGGAAAATACGCTAATTTATTTTTCGTTTGAACTTGACCTGTCTGATTTGTGTTAATCTGCGCGGTGTATATGTTTTTGTAAGTCATGTCGTTTGATATAATCGATAGTTACTAATGTAGTAGTATGTATGATATGTGATATTGTTGTTCCCCCAAAACTACATATATTTGTTGATGTAGAAGTAGGATTAGTTTGTCATGAATCATGATACGGTTTTTGTGACTTTTGTCTGAGTGTTGAATGGTTGTTTTTAATGGGGAAACATATTGTGTAATGCAACTTTTTGTTCCAGATTTGAGTTTGCATTTATNNTATTTTTGACGATACAGT
TARGET=156,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04754 Ps_contig_mira-and-tgicl-ass_franssen_34532
SEQUENCE=CAACTGCTCTGAGGCATTACTCAAGTTCACAGTATCTCGTAAACGAACCTTTATTCTCCAGGACATAGAATGGGGAAAAGAGGAAGCAAAAACTACCAGAAGAAAGACGTAACCAACGCTGCAACAAGTTCCCGACGCGACCGTCATGTGTTTTCCACTCAAGACATGGACGATGAGATAGATGCCTTTCATAAGCAGAGGGATATTGTTCCACTTGACATAAATGCTGATTCTGCAGAGTCGGATGAAGATGATGAAATACCTATTTTTGACTCCAAGGATATTGATAATGATGATAGTGATGATGATGATGATGATGATAATGATAATGATGATGATGATGATGATGATGATGATGATGATGATGATGGTGGAGGAGGATGAATAGTAGGTAACGGACAAAGGTCTTAATTAATTTAACTAAAAAAGTTTAAGTTAAACGGAACTAAAAAGTAAATTTTAAG
TARGET=300,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3860
PRIMER_LEFT_PENALTY=0.174657
PRIMER_RIGHT_PENALTY=0.211336
PRIMER_LEFT_SEQUENCE=AGAAGAAAGACGTAACCAACGC
PRIMER_RIGHT_SEQUENCE=CTATTCATCCTCCTCCACCATC
PRIMER_LEFT=97,22
PRIMER_RIGHT=387,22
PRIMER_LEFT_TM=59.825
PRIMER_RIGHT_TM=59.789
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.3860
PRIMER_LEFT_1_PENALTY=0.174657
PRIMER_RIGHT_1_PENALTY=0.211336
PRIMER_LEFT_1_SEQUENCE=GAAGAAAGACGTAACCAACGCT
PRIMER_RIGHT_1_SEQUENCE=CTATTCATCCTCCTCCACCATC
PRIMER_LEFT_1=98,22
PRIMER_RIGHT_1=387,22
PRIMER_LEFT_1_TM=59.825
PRIMER_RIGHT_1_TM=59.789
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=0.4898
PRIMER_LEFT_2_PENALTY=0.278428
PRIMER_RIGHT_2_PENALTY=0.211336
PRIMER_LEFT_2_SEQUENCE=GCCTTTCATAAGCAGAGGGATA
PRIMER_RIGHT_2_SEQUENCE=CTATTCATCCTCCTCCACCATC
PRIMER_LEFT_2=183,22
PRIMER_RIGHT_2=387,22
PRIMER_LEFT_2_TM=59.722
PRIMER_RIGHT_2_TM=59.789
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.7150
PRIMER_LEFT_3_PENALTY=0.503696
PRIMER_RIGHT_3_PENALTY=0.211336
PRIMER_LEFT_3_SEQUENCE=GTGTTTTCCACTCAAGACATGG
PRIMER_RIGHT_3_SEQUENCE=CTATTCATCCTCCTCCACCATC
PRIMER_LEFT_3=147,22
PRIMER_RIGHT_3=387,22
PRIMER_LEFT_3_TM=59.496
PRIMER_RIGHT_3_TM=59.789
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=0.7245
PRIMER_LEFT_4_PENALTY=0.513182
PRIMER_RIGHT_4_PENALTY=0.211336
PRIMER_LEFT_4_SEQUENCE=AAGCAGAGGGATATTGTTCCAC
PRIMER_RIGHT_4_SEQUENCE=CTATTCATCCTCCTCCACCATC
PRIMER_LEFT_4=192,22
PRIMER_RIGHT_4=387,22
PRIMER_LEFT_4_TM=59.487
PRIMER_RIGHT_4_TM=59.789
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=196
=
PRIMER_SEQUENCE_ID=SSR_04755 Ps_contig_mira-and-tgicl-ass_franssen_34532
SEQUENCE=CAACTGCTCTGAGGCATTACTCAAGTTCACAGTATCTCGTAAACGAACCTTTATTCTCCAGGACATAGAATGGGGAAAAGAGGAAGCAAAAACTACCAGAAGAAAGACGTAACCAACGCTGCAACAAGTTCCCGACGCGACCGTCATGTGTTTTCCACTCAAGACATGGACGATGAGATAGATGCCTTTCATAAGCAGAGGGATATTGTTCCACTTGACATAAATGCTGATTCTGCAGAGTCGGATGAAGATGATGAAATACCTATTTTTGACTCCAAGGATATTGATAATGATGATAGTGATGATGATGATGATGATGATAATGATAATGATGATGATGATGATGATGATGATGATGATGATGATGATGGTGGAGGAGGATGAATAGTAGGTAACGGACAAAGGTCTTAATTAATTTAACTAAAAAAGTTTAAGTTAAACGGAACTAAAAAGTAAATTTTAAG
TARGET=329,42
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5065
PRIMER_LEFT_PENALTY=0.278428
PRIMER_RIGHT_PENALTY=2.228115
PRIMER_LEFT_SEQUENCE=GCCTTTCATAAGCAGAGGGATA
PRIMER_RIGHT_SEQUENCE=TCCGTTACCTACTATTCATCCTCC
PRIMER_LEFT=183,22
PRIMER_RIGHT=398,24
PRIMER_LEFT_TM=59.722
PRIMER_RIGHT_TM=59.772
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.833
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=2.7318
PRIMER_LEFT_1_PENALTY=0.503696
PRIMER_RIGHT_1_PENALTY=2.228115
PRIMER_LEFT_1_SEQUENCE=GTGTTTTCCACTCAAGACATGG
PRIMER_RIGHT_1_SEQUENCE=TCCGTTACCTACTATTCATCCTCC
PRIMER_LEFT_1=147,22
PRIMER_RIGHT_1=398,24
PRIMER_LEFT_1_TM=59.496
PRIMER_RIGHT_1_TM=59.772
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.833
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=2.7413
PRIMER_LEFT_2_PENALTY=0.513182
PRIMER_RIGHT_2_PENALTY=2.228115
PRIMER_LEFT_2_SEQUENCE=GCAGAGGGATATTGTTCCACTT
PRIMER_RIGHT_2_SEQUENCE=TCCGTTACCTACTATTCATCCTCC
PRIMER_LEFT_2=194,22
PRIMER_RIGHT_2=398,24
PRIMER_LEFT_2_TM=59.487
PRIMER_RIGHT_2_TM=59.772
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.833
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=2.7413
PRIMER_LEFT_3_PENALTY=0.513182
PRIMER_RIGHT_3_PENALTY=2.228115
PRIMER_LEFT_3_SEQUENCE=AAGCAGAGGGATATTGTTCCAC
PRIMER_RIGHT_3_SEQUENCE=TCCGTTACCTACTATTCATCCTCC
PRIMER_LEFT_3=192,22
PRIMER_RIGHT_3=398,24
PRIMER_LEFT_3_TM=59.487
PRIMER_RIGHT_3_TM=59.772
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.833
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=2.8110
PRIMER_LEFT_4_PENALTY=0.582904
PRIMER_RIGHT_4_PENALTY=2.228115
PRIMER_LEFT_4_SEQUENCE=CGTCATGTGTTTTCCACTCAAG
PRIMER_RIGHT_4_SEQUENCE=TCCGTTACCTACTATTCATCCTCC
PRIMER_LEFT_4=141,22
PRIMER_RIGHT_4=398,24
PRIMER_LEFT_4_TM=60.583
PRIMER_RIGHT_4_TM=59.772
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.833
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_04756 Ps_contig_mira-and-tgicl-ass_franssen_34535
SEQUENCE=CTTGATCCAACCAACCTGCATTCACCAAAATGGATTCCCGCTCACCTCCACCACCACCAACCATGATGGTGGGACCCACATCTTACGCTCCGACTAACACCTCCATGATCAGCCCTAATTCCTCCACCACCAACATCATGTCTCCGGCCACCGCTCGTTTTCCTTTCACTTCGCAGCAGCAACCGGACCCGTTCGCCGTCACTCACGACAGCCCTTCTTCGCCGTCCACCCTCGCCAAGAAAAAGCGTGGCCGGCCGAGGAAATACTCCCCGGACGGTAACATTGCCCTTGCTTTGGCTCCGAATCGAGTTTCTTCCCCTGCCGCTGCTACATCCGCTTCTGCTGGAGATTCGGGAAACGCGGAGGCTCCTGCCAAGAAACATAGAGGAAGACCTCCTGGCTCTGGGAAGAAACAGTTAGATGCGCTTGGAGCTGGTGGGATTGGATTCACTCCACACGTAGTCTTGGTGGAGTCTGGTGAGGACATTACGGAAAAAGTTATGGCCTTTAGTCAGTCAGGCCCTCGAACAGTTTGTATTCTTTCTGCCAATGGTGCAGTCAGCAGTGCAACCCTTCGACAGCCATCTGGTGGTATTGCAAGATACGAGGGCCAATTTGAGATCATATCGTTATCAGGTGATATGCACCTATCTGAAAACAGTGGTGAGCATAGCAGACCAAGCAGTTTATACGTTTCTCTTGCAGGGGCTGATGGACGAGTTTTGGGTGGTGCAGTTGCTGGAGTGCTAATTGCAGCCTCAACGATACAGGTCATTGTGGGTAGCTTTATTATGGATTTGAAAAAGTCAAGCTCAAGTATGGTGAAATCTGGGCCTTCTTCAGCACCAACTTCCCAAATGTTGAACTTCGGTGCACCTACAACTCCAACCAGTCCTACTTCTCAAGGGCCTTCAACTGAGTCATCGGAGGAAAATGATCATAATAGTAATTTTAGTAGGGGGCCTGCACTCTACAACAATGCTAATCAGCCTGTTCATAATAATATGCAGCAGATGTACCACCATCCACTATGGGCCGGCCAAACTCATCAGTGAAGACGAGACTTTGGATTAACAGTGCAGAGGCAGGGTATTTGTTGGATGATTGTTCAGAGTTTAGTGTCTAGAGGTCAAGTCTAAAATGCTAACATGCATTATTGAGGACTGACATTAGAACAGTCTGTATTAGTAAGGTTTAAGTTAATCTCCATTGCTCAGCCAGTTAGGATGTTTTGTTCGTGTATCCTTTAATTATATATATTAGTAGTGATTCTAATGATGCTGGCG
TARGET=48,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6864
PRIMER_LEFT_PENALTY=1.576890
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=CTGCATTCACCAAAATGGATTC
PRIMER_RIGHT_SEQUENCE=ATGTTGGTGGTGGAGGAATTAG
PRIMER_LEFT=15,22
PRIMER_RIGHT=135,22
PRIMER_LEFT_TM=61.577
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=2.3905
PRIMER_LEFT_1_PENALTY=2.281005
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=CATTCACCAAAATGGATTCCC
PRIMER_RIGHT_1_SEQUENCE=ATGTTGGTGGTGGAGGAATTAG
PRIMER_LEFT_1=18,21
PRIMER_RIGHT_1=135,22
PRIMER_LEFT_1_TM=61.281
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=2.4050
PRIMER_LEFT_2_PENALTY=2.295472
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=CAACCTGCATTCACCAAAATG
PRIMER_RIGHT_2_SEQUENCE=ATGTTGGTGGTGGAGGAATTAG
PRIMER_LEFT_2=11,21
PRIMER_RIGHT_2=135,22
PRIMER_LEFT_2_TM=61.295
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=2.4354
PRIMER_LEFT_3_PENALTY=2.325937
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=GATCCAACCAACCTGCATTC
PRIMER_RIGHT_3_SEQUENCE=ATGTTGGTGGTGGAGGAATTAG
PRIMER_LEFT_3=3,20
PRIMER_RIGHT_3=135,22
PRIMER_LEFT_3_TM=60.326
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=2.5448
PRIMER_LEFT_4_PENALTY=1.576890
PRIMER_RIGHT_4_PENALTY=0.967916
PRIMER_LEFT_4_SEQUENCE=CTGCATTCACCAAAATGGATTC
PRIMER_RIGHT_4_SEQUENCE=GATGTTGGTGGTGGAGGAATTA
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=136,22
PRIMER_LEFT_4_TM=61.577
PRIMER_RIGHT_4_TM=60.968
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=122
=
PRIMER_SEQUENCE_ID=SSR_04757 Ps_contig_mira-and-tgicl-ass_franssen_34537
SEQUENCE=GTTCGGGGAAGGTCACACAATAAGCAACCCTTCCCTCTTTCACATTTCTTGTCCAATCTCCACCCAAACCACCCCTAACCCTTTTTCTTTTCCTTTACACACAATTCTTTTTTTCAAAGAGAGAGAAATCTCTCCCTCTTTCTCCCCTCTCATCAATGGAAATCAGTTCTAGATTTCACCATGAAAGGGAGAATCCATCTCCCATGAACTCACCAAAAAGCAATGTAAGTAACTGTATGAGTAGCAGCAACAGCAACAATGGAATCCAAATTCAAACACCACCTCTCACTCCAAAAACAATCCCTAGATCTGATTCAAATCCTTACCCTACAACCTTTGTTCAAGCTGATACAACAACTTTCAAACAAGTTGTTCAAATGTTAACAGGTTCATCAGAAACAACAACCTCGACCACCACAACGACAACAACAACAACCTCAACCACAACAAGACCAAATCAAGATCTACCTCAACATCCAACAAGAAACTTCAACATACCTCCAATCAAAACAGCACCAAAGAAACAAGGTTTCAAGTTATATGAGAGAAGGAACAGTCTCAAGAACAGTGTTCTTATGCTCAACACCCTCATGCCAAATGTTTTACCTAGGAAACAACAACAACAGTATCAACAAGAGATTCTCTCTCCGAGCTTGCTTGATTTTCCTTCTCTTGCTCTTAGCCCTGTCACACCATTGAATGATGACCCTTTTGATAAATCTTCACCTTCATTGGGAAATTCATCCTCCGAAGAAGATAAAGCTATAGCTGAAAAAGGTTTCTATTTGCACCCCTCTCCTATTACTACACCAAGAGACTCAGAGCCACAGCTTTTGCCTCTCTTCCCTCTCTCTTCACCTAGACTCTCACAATCAAGTTCTTGAGGAAGAAATAATCAATTCTTGTGTAAAAGATTGAAACTTGCTATGTGGGTGGTTTAGCTTCACAAATTTACAACATGGGTTTTGAAATTTGAATCATTTCAAACAAGGTAAAATACATAGCTTGGTTTTATTTGTAAAAGTGAATTTCTTAGTCTTACCATGAAATGGTCAACATCCTTTTTCTCTTCTTTACTTCTCATGTTAACTTTGTCTTCAAATTCTAAATATTTGCTATCATTTACCTTTCCATAAATGAAAGAAGAGGAAAAAAGTCTCTTTGTAACC
TARGET=423,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0165
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=CAAATTCAAACACCACCTCTCA
PRIMER_RIGHT_SEQUENCE=TCTTGATTTGGTCTTGTTGTGG
PRIMER_LEFT=266,22
PRIMER_RIGHT=462,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=0.0271
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=CTCTCACTCCAAAAACAATCCC
PRIMER_RIGHT_1_SEQUENCE=TGCTGTTTTGATTGGAGGTATG
PRIMER_LEFT_1=282,22
PRIMER_RIGHT_1=514,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.3000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.0271
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.003589
PRIMER_LEFT_2_SEQUENCE=TCTCACTCCAAAAACAATCCCT
PRIMER_RIGHT_2_SEQUENCE=TGCTGTTTTGATTGGAGGTATG
PRIMER_LEFT_2=283,22
PRIMER_RIGHT_2=514,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.0681
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=AGATTTCACCATGAAAGGGAGA
PRIMER_RIGHT_3_SEQUENCE=TCTTGATTTGGTCTTGTTGTGG
PRIMER_LEFT_3=170,22
PRIMER_RIGHT_3=462,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.0831
PRIMER_LEFT_4_PENALTY=0.008257
PRIMER_RIGHT_4_PENALTY=0.074807
PRIMER_LEFT_4_SEQUENCE=CAAATTCAAACACCACCTCTCA
PRIMER_RIGHT_4_SEQUENCE=AACCTTGTTTCTTTGGTGCTGT
PRIMER_LEFT_4=266,22
PRIMER_RIGHT_4=530,22
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_04758 Ps_contig_mira-and-tgicl-ass_franssen_34537
SEQUENCE=GTTCGGGGAAGGTCACACAATAAGCAACCCTTCCCTCTTTCACATTTCTTGTCCAATCTCCACCCAAACCACCCCTAACCCTTTTTCTTTTCCTTTACACACAATTCTTTTTTTCAAAGAGAGAGAAATCTCTCCCTCTTTCTCCCCTCTCATCAATGGAAATCAGTTCTAGATTTCACCATGAAAGGGAGAATCCATCTCCCATGAACTCACCAAAAAGCAATGTAAGTAACTGTATGAGTAGCAGCAACAGCAACAATGGAATCCAAATTCAAACACCACCTCTCACTCCAAAAACAATCCCTAGATCTGATTCAAATCCTTACCCTACAACCTTTGTTCAAGCTGATACAACAACTTTCAAACAAGTTGTTCAAATGTTAACAGGTTCATCAGAAACAACAACCTCGACCACCACAACGACAACAACAACAACCTCAACCACAACAAGACCAAATCAAGATCTACCTCAACATCCAACAAGAAACTTCAACATACCTCCAATCAAAACAGCACCAAAGAAACAAGGTTTCAAGTTATATGAGAGAAGGAACAGTCTCAAGAACAGTGTTCTTATGCTCAACACCCTCATGCCAAATGTTTTACCTAGGAAACAACAACAACAGTATCAACAAGAGATTCTCTCTCCGAGCTTGCTTGATTTTCCTTCTCTTGCTCTTAGCCCTGTCACACCATTGAATGATGACCCTTTTGATAAATCTTCACCTTCATTGGGAAATTCATCCTCCGAAGAAGATAAAGCTATAGCTGAAAAAGGTTTCTATTTGCACCCCTCTCCTATTACTACACCAAGAGACTCAGAGCCACAGCTTTTGCCTCTCTTCCCTCTCTCTTCACCTAGACTCTCACAATCAAGTTCTTGAGGAAGAAATAATCAATTCTTGTGTAAAAGATTGAAACTTGCTATGTGGGTGGTTTAGCTTCACAAATTTACAACATGGGTTTTGAAATTTGAATCATTTCAAACAAGGTAAAATACATAGCTTGGTTTTATTTGTAAAAGTGAATTTCTTAGTCTTACCATGAAATGGTCAACATCCTTTTTCTCTTCTTTACTTCTCATGTTAACTTTGTCTTCAAATTCTAAATATTTGCTATCATTTACCTTTCCATAAATGAAAGAAGAGGAAAAAAGTCTCTTTGTAACC
TARGET=613,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1547
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.151106
PRIMER_LEFT_SEQUENCE=CATACCTCCAATCAAAACAGCA
PRIMER_RIGHT_SEQUENCE=GACAGGGCTAAGAGCAAGAGAA
PRIMER_LEFT=493,22
PRIMER_RIGHT=688,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.151
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=196
PRIMER_PAIR_PENALTY_1=0.1594
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.151106
PRIMER_LEFT_1_SEQUENCE=CCACAACAAGACCAAATCAAGA
PRIMER_RIGHT_1_SEQUENCE=GACAGGGCTAAGAGCAAGAGAA
PRIMER_LEFT_1=441,22
PRIMER_RIGHT_1=688,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.1635
PRIMER_LEFT_2_PENALTY=0.074807
PRIMER_RIGHT_2_PENALTY=0.088662
PRIMER_LEFT_2_SEQUENCE=AACAGCACCAAAGAAACAAGGT
PRIMER_RIGHT_2_SEQUENCE=TAGGAGAGGGGTGCAAATAGAA
PRIMER_LEFT_2=508,22
PRIMER_RIGHT_2=800,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=60.089
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.1635
PRIMER_LEFT_3_PENALTY=0.074807
PRIMER_RIGHT_3_PENALTY=0.088662
PRIMER_LEFT_3_SEQUENCE=ACAGCACCAAAGAAACAAGGTT
PRIMER_RIGHT_3_SEQUENCE=TAGGAGAGGGGTGCAAATAGAA
PRIMER_LEFT_3=509,22
PRIMER_RIGHT_3=800,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=60.089
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=292
PRIMER_PAIR_PENALTY_4=0.1665
PRIMER_LEFT_4_PENALTY=0.115769
PRIMER_RIGHT_4_PENALTY=0.050763
PRIMER_LEFT_4_SEQUENCE=ACCCTCATGCCAAATGTTTTAC
PRIMER_RIGHT_4_SEQUENCE=TGGCTCTGAGTCTCTTGGTGTA
PRIMER_LEFT_4=584,22
PRIMER_RIGHT_4=826,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=60.051
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_04759 Ps_contig_mira-and-tgicl-ass_franssen_34538
SEQUENCE=AACACCATTTCCGTCTNCNTCTNGGGTCTGAAAAACTCAACACTGAACGCGAAAACGAAAAGCAAAAGGGAATTAGGGTTTAAGAAACCGCAATGGATTTCACATCAGCGAAGAACGCATCAGAGAACAACAACAACAACAACAACTCAGAAAACTCTCACCAAATCGCTGATTCTTCTCCTCAATTAGCTTCTGATTCCCACAATATCAAAGAAACCGAAGAGCGACAATCACGTGAACTCAAAGCTGGTCTTCACCCTCTAAAGAGCAAGTTTGTATTTTGGTACACTCGTCGGGTACCTGGGATTCGGAATCAGTCATATGAGGACAACATAAAGAAGATTGTTGAGTTCAGTACGGTTGAAGGATTTTGGGTCTGTTATTGCCATATTTCTCGTCCTGCTTCTTTGCCTAGTCCAACAGATTTGCACCTTTTTAAAGAAGGAATTCGTCCGTTATGGGAGGACTCTGCTAACTGCAATGGTGGAAAATGGATTATACGATTCAAAAAGGCTGTCTCAGGTCGTTTTTGGGAGGACCTGGTGCTTGCTTTAGTGGGTGACCAGTTGGATTATGGTGATAACATATGCGGTGCAGTACTAAGCATTCGATTCAACGAGGATATAGTGAGTGTGTGGAACCGCAATGCTTCTGATAATCAGGCTGTAATGGCTTTGAGAGATTCGATTAAGCGTCACTTAAAGCTTCCTCACAGCTATGTTATGGAGTACAAGCCCCACGACGCTTCTCTGCGAGACAATTCATCTTACAGGAATACTTGGTTGAGAGGCTAGAGATACAACTGTGCTTGTCCAAGTCCCCTTGACTGACGCTTTTGTATACTTGTGATCATTGGCCACAGTCTAATGATGATGTTGGGTGTTTCAGGACTGGTTCTGACTTTTGAATTAAATGTACGAAGCAACATTTTGAATGCTTTATTGAAAGTTTCATTTGGTTACTTGAGGATTCACTTACTTTGTTTTTGTTCTTGCTTTGTTGTTGTAAATAACTTTACCCGGTTTGTCTTTTCAACTTGCCGAAGGAAAGAGGCATGGCTCCAGTCCTGAAACCCTAATAAGATGCATTAAAAAAAACTCGGACTGGTTG
TARGET=126,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3341
PRIMER_LEFT_PENALTY=0.289260
PRIMER_RIGHT_PENALTY=0.044832
PRIMER_LEFT_SEQUENCE=GAAAAGCAAAAGGGAATTAGGG
PRIMER_RIGHT_SEQUENCE=TGTCCTCATATGACTGATTCCG
PRIMER_LEFT=56,22
PRIMER_RIGHT=329,22
PRIMER_LEFT_TM=60.289
PRIMER_RIGHT_TM=59.955
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.3696
PRIMER_LEFT_1_PENALTY=0.289260
PRIMER_RIGHT_1_PENALTY=0.080323
PRIMER_LEFT_1_SEQUENCE=GAAAAGCAAAAGGGAATTAGGG
PRIMER_RIGHT_1_SEQUENCE=CCGACGAGTGTACCAAAATACA
PRIMER_LEFT_1=56,22
PRIMER_RIGHT_1=295,22
PRIMER_LEFT_1_TM=60.289
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=0.3925
PRIMER_LEFT_2_PENALTY=0.347655
PRIMER_RIGHT_2_PENALTY=0.044832
PRIMER_LEFT_2_SEQUENCE=TAGGGTTTAAGAAACCGCAATG
PRIMER_RIGHT_2_SEQUENCE=TGTCCTCATATGACTGATTCCG
PRIMER_LEFT_2=73,22
PRIMER_RIGHT_2=329,22
PRIMER_LEFT_2_TM=60.348
PRIMER_RIGHT_2_TM=59.955
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.4037
PRIMER_LEFT_3_PENALTY=0.289260
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=GAAAAGCAAAAGGGAATTAGGG
PRIMER_RIGHT_3_SEQUENCE=GCTCTTTAGAGGGTGAAGACCA
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=268,22
PRIMER_LEFT_3_TM=60.289
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.4037
PRIMER_LEFT_4_PENALTY=0.289260
PRIMER_RIGHT_4_PENALTY=0.114470
PRIMER_LEFT_4_SEQUENCE=GAAAAGCAAAAGGGAATTAGGG
PRIMER_RIGHT_4_SEQUENCE=TCTTTAGAGGGTGAAGACCAGC
PRIMER_LEFT_4=56,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=60.289
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_04760 Ps_contig_mira-and-tgicl-ass_franssen_34548
SEQUENCE=GGTGACCGTACGGTTTCTCGTGTAACATCCTTCCACCGTTTTGCAAACGCATGCGCAAGCGACGACATTTTGATGGTGCGGCCATTGATCCTCAGCAACGTCAACTCGTTTAATAATCTCCGCAAGTAGATCCGGCAGCATGGTTGACCAATCAGTTCCGTCCTCCGATTCACGGACGGCGGTTCCTTTCCGGTCATCGCCGGTAGCATCGAGAGATGGAACCTCGTTTAAGGAACTCGAGAATTTGCCAGATCGAAAAACCTTCATCAACGACATGTGTAATAACAATGGTTCTAGAGAGAATGATTCTAGAGAGAGAGAGTTGATTTAACCATCCACAGAA
TARGET=311,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04761 Ps_contig_mira-and-tgicl-ass_franssen_34550
SEQUENCE=ATAACTTGTCTTGTTCGGAAAGAATTCGCCCAATCCAAGACACGAATATTGTATCGCATTAACCCTAATTTTTACAATTCGATTTCAATTGCTATTGTATTTTGGGGAAGATCAAGCAAATACAGAAAAAGCATAAGAAGAAACAAAACCTAAACGACGACGACGCAGTTTTGAACGATGTCTGCTGCGGTGTGCGGAACCAAGAGATCTTTCTTCGAAGACCTTCCTCCTTCACCACCTGTTTCCAAGAGGCTTCGTTGTTCATCTTCTCCGATTCGCCTTTCTCTTCCGACGCTCGTTGATCACCTTCGCAATCTCTTTCCCAATATGGAAGATCAGATCCTTGAAAGAGCATTGCAGGAATGCGGTAATGATTTAGATGCTGCTATTAAAAGTTTGCACGGGCTTTGCTTGGGATCTGCCGATGAAATTGCCGCAATTGCTCCACAGCCTGATGTTGTTACCGTTGAATCAGGTGTTTTTGAAAATAATGGGGATGCATCTGCCTCTGGGAATCAGCCAGTTGAAAACAATCTTCCTGCTGATGGACCTGAATGGATTGACTTGTTTGTTAGAGAAATGTCATGTGCTACTAGTGTTGATGATGCCAGAGCTCGTGCTGCGAAACTTCTTGAGGTTCTGGAGAAATCGATCAGTGCACATGCGAGTTCTGGGGCATTAACTGATCTTCAAAGGGAAAATCTGATGTTGAAATATCAAGTTGAAGTCTTGACCAAGGAGAGAAATTGTTTTAAAAGTGCTTTTAGAATCCAGCTTGAGCGCCTTTCAGATTATGAAGATAAAGACCGAGAGTTGCAGCAGCTGAAGCAGTTGGTATCTCAATACCAGGAACAGATCCGGACTCTTGAGGTTAACAACTATGCTCTGCGAATGCATCTTAATCAGGCACAAAAATACAACCCCTTTCCCGGGCGTTTCCCTCCCGACGGCTTCTAATGCAATCGGCGGGTAGAGTTTCACAATCAAGGCAGTTAAATAGCTGTATCTGGCTAATTAATTATATCCCATGTTTTATTTTTCCAAAAGAAGTATGTATTGTTTATTGTTACAATTTGTGTAGCTCCTGTAGGTCAAGGAAATGTAGCGCTATTCAGGGGTGAATCTATCTTATCCTGGAATAGAACCGATGATAATAATTCGTTATTTATTCCTCACTGAATTGATTTCTGGTATTATATGTACTAAAATTACTGAGAATGTTCAAGACCAGTTTGTTACCATCACTATACTACAAATTATGTTTTTA
TARGET=154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1423
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.108206
PRIMER_LEFT_SEQUENCE=GGGGAAGATCAAGCAAATACAG
PRIMER_RIGHT_SEQUENCE=GAAGATGAACAACGAAGCCTCT
PRIMER_LEFT=103,22
PRIMER_RIGHT=268,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.1423
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.108206
PRIMER_LEFT_1_SEQUENCE=GGGGAAGATCAAGCAAATACAG
PRIMER_RIGHT_1_SEQUENCE=GAGAAGATGAACAACGAAGCCT
PRIMER_LEFT_1=103,22
PRIMER_RIGHT_1=270,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=168
PRIMER_PAIR_PENALTY_2=0.1502
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.116088
PRIMER_LEFT_2_SEQUENCE=GGGGAAGATCAAGCAAATACAG
PRIMER_RIGHT_2_SEQUENCE=CAGCATCTAAATCATTACCGCA
PRIMER_LEFT_2=103,22
PRIMER_RIGHT_2=384,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=60.116
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=11.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.3140
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.279852
PRIMER_LEFT_3_SEQUENCE=GGGGAAGATCAAGCAAATACAG
PRIMER_RIGHT_3_SEQUENCE=TCCATATTGGGAAAGAGATTGC
PRIMER_LEFT_3=103,22
PRIMER_RIGHT_3=331,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=60.280
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=0.4204
PRIMER_LEFT_4_PENALTY=0.312177
PRIMER_RIGHT_4_PENALTY=0.108206
PRIMER_LEFT_4_SEQUENCE=TTGGGGAAGATCAAGCAAATAC
PRIMER_RIGHT_4_SEQUENCE=GAAGATGAACAACGAAGCCTCT
PRIMER_LEFT_4=101,22
PRIMER_RIGHT_4=268,22
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=59.892
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=168
=
PRIMER_SEQUENCE_ID=SSR_04762 Ps_contig_mira-and-tgicl-ass_franssen_34562
SEQUENCE=ATCTTCTCGTCTTTTGAACGCCATTCTTGTTTCTTTCATCTTTTACTGATGCAGTTTTCTTCTTTACACGGGCGCTCATCATATCCTCAATAATATGAAGAGATGACCTTATGGTTGACCATAACTGGACTCTATGAATCTTAGTCACTTCAGATGCTGGTAACTCCTCCTTTTGACTTCCATTTTCAATTGTACTATCTTCACTGCCAGGGACGTGGCCGCTTGTTTGATGTCCATCAACACCCTTCTCTAAACTCGCATCAATTTCTTCCCCGACAACCACGTCCCCAGAAACTTTCTCATCTTCTCCCACAGCTGAGGCCAATTCAGAAGACTCCGTCTCTGCCTGCCTATCTAAAAATGACTTCCACTTCTCTGACCTTTCCTCCTCTTCCTCCTTGTATATATTCGCATATTCACGGTATCTTTGCAGGTGCTGAGGTCTCACCGTAAACCCATAGGCATCCCTCTTGTGATCGAAAGTAATAACAGGATTTGAAGTTTTGTTAGTTTTCATTTCGTAGATTTGATTTGAATCAGAAGAGAAGAAATAGGGTTGAAAATACGAAGATCTCGTTTTACTTGATTTTCTGCAACTGTATTTTGTTAAGCAGCAGCTAGTAAGAAGAAGAGTGAGAAAGTTGAAGAGAGAGAAAGAGAGAGAGCACATGCATAGAGAGAGAAAGAGCGAAAATGGCGT
TARGET=656,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3999
PRIMER_LEFT_PENALTY=0.014792
PRIMER_RIGHT_PENALTY=0.385098
PRIMER_LEFT_SEQUENCE=TCGCATATTCACGGTATCTTTG
PRIMER_RIGHT_SEQUENCE=CCATTTTCGCTCTTTCTCTCTC
PRIMER_LEFT=408,22
PRIMER_RIGHT=696,22
PRIMER_LEFT_TM=59.985
PRIMER_RIGHT_TM=59.615
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.6266
PRIMER_LEFT_1_PENALTY=0.241544
PRIMER_RIGHT_1_PENALTY=0.385098
PRIMER_LEFT_1_SEQUENCE=GAGGTCTCACCGTAAACCCATA
PRIMER_RIGHT_1_SEQUENCE=CCATTTTCGCTCTTTCTCTCTC
PRIMER_LEFT_1=438,22
PRIMER_RIGHT_1=696,22
PRIMER_LEFT_1_TM=60.242
PRIMER_RIGHT_1_TM=59.615
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.9741
PRIMER_LEFT_2_PENALTY=0.589035
PRIMER_RIGHT_2_PENALTY=0.385098
PRIMER_LEFT_2_SEQUENCE=AGGTCTCACCGTAAACCCATAG
PRIMER_RIGHT_2_SEQUENCE=CCATTTTCGCTCTTTCTCTCTC
PRIMER_LEFT_2=439,22
PRIMER_RIGHT_2=696,22
PRIMER_LEFT_2_TM=59.411
PRIMER_RIGHT_2_TM=59.615
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=1.3322
PRIMER_LEFT_3_PENALTY=0.014792
PRIMER_RIGHT_3_PENALTY=1.317432
PRIMER_LEFT_3_SEQUENCE=TCGCATATTCACGGTATCTTTG
PRIMER_RIGHT_3_SEQUENCE=GCCATTTTCGCTCTTTCTCTCT
PRIMER_LEFT_3=408,22
PRIMER_RIGHT_3=697,22
PRIMER_LEFT_3_TM=59.985
PRIMER_RIGHT_3_TM=61.317
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=1.4802
PRIMER_LEFT_4_PENALTY=0.014792
PRIMER_RIGHT_4_PENALTY=1.465403
PRIMER_LEFT_4_SEQUENCE=TCGCATATTCACGGTATCTTTG
PRIMER_RIGHT_4_SEQUENCE=GCCATTTTCGCTCTTTCTCTC
PRIMER_LEFT_4=408,22
PRIMER_RIGHT_4=697,21
PRIMER_LEFT_4_TM=59.985
PRIMER_RIGHT_4_TM=60.465
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_04763 Ps_contig_mira-and-tgicl-ass_franssen_34568
SEQUENCE=GGCCAATTCCTATTTTCTAACGAGAGCTGTGATATTCAAAATCCCAAGCAAATAAACATCTAAACGACGTCGTTTCTTCTGCAACACTTTCTCAGTTTCTCTCTCAGAATTGGCCATGGCGAACGAGAACGAACCCGCGAAACTCCTCTTACCATACCTTCAACGCGCCGATGAATTGCAGAAACATGAACCTCTCGTCGCTTATTACTGTCGATTATATGCGATGGAGCGTGGATTGAAGATTCCACAAAGTGATCGCACCAAAACCACTAATTCTCTTCTTGTTTCACTCATGAAGCAGCTCGAAAAGGATAAGAAATCAATCCAGCTGGGGCCTGAAGATAATTTATATCTTGAGGGATTTTCTTTGAATGTGTTTGGAAAAGCAGACAAGCAAGACCGTGCTGGAAGAGCAGATATAAGTACGGCAAAAACATTTTATGCTGCTAGCATCTTTTTTGAGATTCTTAATCAATTTGGACCAGTTCAGCCTGATCTGGAGCAGAAGCAGAAGTATGCAGCATGGAAAGCAGCTGATATAAGAAAGGCTTTGAAAGAAGGAAGGAAGCCCGTGGCGGGTCCACCTGCTGGCGACGAGGACCTGTCTGTTCCATCGAGTTCTCCTAGTATTAGATATGATCCTGGGGCTACCGAAACTTCTGCTTCCAGTGCTGGATCAGAATCTGATTCAACACGTAGTTATCATAACCCTGTCAACTTCCAGAATATGCCGAGCATTCATCCTCCACCTAAATTCAATGATACTGTTAATGATCAGAACTCTGCAAACATTCCACCATCTTCACAGTTTCATGATAGGGTAGATGATAATAAGCATTCTTCAATTGTTTCTCCATCATCTCACTCTTATACACCTGGAGTTTATCCTTCTCAAGACTTCCATCCACCTCCATCTTCCCAGGATTATCATCATCCACCTCCATCTTCCCAAGATTATCATCATCCACCACCATCCCAGGATTATCACCGTCCACCATCTTCCCAAGATTACCATCCTCCACCACCAAACACTCCTCTCCAACATCAT
TARGET=557,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3503
PRIMER_LEFT_PENALTY=0.095538
PRIMER_RIGHT_PENALTY=0.254773
PRIMER_LEFT_SEQUENCE=CAGAAGTATGCAGCATGGAAAG
PRIMER_RIGHT_SEQUENCE=AGGAGAACTCGATGGAACAGAC
PRIMER_LEFT=508,22
PRIMER_RIGHT=624,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=59.745
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=117
PRIMER_PAIR_PENALTY_1=0.3905
PRIMER_LEFT_1_PENALTY=0.095538
PRIMER_RIGHT_1_PENALTY=0.294975
PRIMER_LEFT_1_SEQUENCE=CAGAAGTATGCAGCATGGAAAG
PRIMER_RIGHT_1_SEQUENCE=ACTGGAAGCAGAAGTTTCGGTA
PRIMER_LEFT_1=508,22
PRIMER_RIGHT_1=669,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=60.295
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.4154
PRIMER_LEFT_2_PENALTY=0.160609
PRIMER_RIGHT_2_PENALTY=0.254773
PRIMER_LEFT_2_SEQUENCE=GATCTGGAGCAGAAGCAGAAGT
PRIMER_RIGHT_2_SEQUENCE=AGGAGAACTCGATGGAACAGAC
PRIMER_LEFT_2=493,22
PRIMER_RIGHT_2=624,22
PRIMER_LEFT_2_TM=60.161
PRIMER_RIGHT_2_TM=59.745
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=0.4556
PRIMER_LEFT_3_PENALTY=0.160609
PRIMER_RIGHT_3_PENALTY=0.294975
PRIMER_LEFT_3_SEQUENCE=GATCTGGAGCAGAAGCAGAAGT
PRIMER_RIGHT_3_SEQUENCE=ACTGGAAGCAGAAGTTTCGGTA
PRIMER_LEFT_3=493,22
PRIMER_RIGHT_3=669,22
PRIMER_LEFT_3_TM=60.161
PRIMER_RIGHT_3_TM=60.295
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=0.5119
PRIMER_LEFT_4_PENALTY=0.095538
PRIMER_RIGHT_4_PENALTY=0.416367
PRIMER_LEFT_4_SEQUENCE=CAGAAGTATGCAGCATGGAAAG
PRIMER_RIGHT_4_SEQUENCE=CTGGAAGCAGAAGTTTCGGTAG
PRIMER_LEFT_4=508,22
PRIMER_RIGHT_4=668,22
PRIMER_LEFT_4_TM=59.904
PRIMER_RIGHT_4_TM=60.416
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_04764 Ps_contig_mira-and-tgicl-ass_franssen_34569
SEQUENCE=TATCATAACAAAGGGTAATAAGCARNRNTANNNATNCAGCANTTACGCGGGGATCGGGGATTCACTCCAAGATACCAACACTATTCACACCTCGCTCCCAGCTAGGGTTTATCGAGGGTTTCGCACTCTCTATCTCCGACAATGGCGAAACCTGGGAGAGGTCGTCGCTCACCACCGTCGGGTTCCGTCTCCGGATCTTCCTCTCTCTCATCCTCTTCTCGTTCCTCTTCTCGCTCTCGTTCTCGCTCCAGATCCTTCTCTTCCTCCTCCTCCCGCAGTTCCAAGAGCCGCAGCCGGAGTCCTCCTCCTCAGAGAAGGAAAAGTTCCACTGAACCAGCTAGGCGAGGTCGATCTCCTGCTCTTCCTCCCCCTCCTCAGTCTAAGAAAGCTTCTCCTCCCCCAAGGAAACCTTCTCCTGTACGCGAGTCTCTTGTACTGCACGTTGAAAAACTCAGCAGGAATGTCAATGAAGGCCATTTGAAAGAAATCTTCAGTAATTTTGGTGAGGTTGTAAGTGTGGAGTTGGTGATGGATAGAGCTGTTAATCTTCCAAAAGGATATGGGTATGTGCATTTCAAGACAAGAGGAGAAGCTGAAAAAGCATTAAATTTCATGGATGGTGCCCAAATAGATGGCAATGTCATTAAGGCAAGATTTACTCTACCTCCAAGGCACAAAGTTTCACCACCTCCAAAAGCTTCTGCTCTTGCTCCAAAGAGAGATGCACCTAGGACTGACAATGCTGGTGCAGATGTTGAAAAAGATGGGCCAAAGCGTCCAAGAGAATCCCCTCCTCGGAGAAAACTTCCTCCCTCACCACGAAGAAGATCTCCTGTGCCTCGAAGAGCTGGATCTCCAAGACGACCAGAATCTCCTCGACGCCGTGCAGATTCTCCTGTCCGTCGTCGATTAGATTCTCCTTATCGCCGTGGAGCTGGAGACACACCTCCTAGGAGGAGACCTGTGTCTCCTGGTAGAGGTCGTTCTCCATCTCCCCCACCTAGGCGCTTGAGATCCCCTGCAAGAGTCTCACCTCGAAGAGTGCGTGGAAGCCCAGGCCCAGGTCGGAGACGTACTCCTCCTCCACCTCCAAGACGCCGTTCACCTCCCAGAAGAGCTCGTAGTCCTCCTAGAAGATCTCCAGTTGGTCGTAGGCGTAGTCGCTCTCCAATACGTAGATCTGCACGCTCTCGCTCAAGATCGTTCTCACCTCGCCGAGGTCGGCCACCTGTGAGACGGGGAAGATCATCATCTTACTCTGATTCACCAAGTCCTCGTAAGGTATCCCGGAGATCAAAGAGTCGAAGTCCAAGAAGGCCTTTGAAAGGAAGAGCTAGTAGCAACAGTAGCAGCAGCAGCTCACCACCTCCAGCCCGCAAGCCATAGATAATTCCTGTATATCTGTTTCCTCTGATATATTTATAAGGATTCTGTACTAGCAACTAAGAGTTATTCTAGCTGTAAGGGTGCTTGGGGGTATTTGTTGCATCTTATGGTTGTCTATTGTCTAATACATATTAGGGATTCAAGTAGTTTGAGAACTCAGTTACATTATTCTGCGTTTGGTTTTTGACTTTAGCAATGCTGTTTTTCTTTGTAATACAAATTTGGAAATGGTATTTTTATCCCTTTGCATAACCAGTTACAA
TARGET=262,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04765 Ps_contig_mira-and-tgicl-ass_franssen_34569
SEQUENCE=TATCATAACAAAGGGTAATAAGCARNRNTANNNATNCAGCANTTACGCGGGGATCGGGGATTCACTCCAAGATACCAACACTATTCACACCTCGCTCCCAGCTAGGGTTTATCGAGGGTTTCGCACTCTCTATCTCCGACAATGGCGAAACCTGGGAGAGGTCGTCGCTCACCACCGTCGGGTTCCGTCTCCGGATCTTCCTCTCTCTCATCCTCTTCTCGTTCCTCTTCTCGCTCTCGTTCTCGCTCCAGATCCTTCTCTTCCTCCTCCTCCCGCAGTTCCAAGAGCCGCAGCCGGAGTCCTCCTCCTCAGAGAAGGAAAAGTTCCACTGAACCAGCTAGGCGAGGTCGATCTCCTGCTCTTCCTCCCCCTCCTCAGTCTAAGAAAGCTTCTCCTCCCCCAAGGAAACCTTCTCCTGTACGCGAGTCTCTTGTACTGCACGTTGAAAAACTCAGCAGGAATGTCAATGAAGGCCATTTGAAAGAAATCTTCAGTAATTTTGGTGAGGTTGTAAGTGTGGAGTTGGTGATGGATAGAGCTGTTAATCTTCCAAAAGGATATGGGTATGTGCATTTCAAGACAAGAGGAGAAGCTGAAAAAGCATTAAATTTCATGGATGGTGCCCAAATAGATGGCAATGTCATTAAGGCAAGATTTACTCTACCTCCAAGGCACAAAGTTTCACCACCTCCAAAAGCTTCTGCTCTTGCTCCAAAGAGAGATGCACCTAGGACTGACAATGCTGGTGCAGATGTTGAAAAAGATGGGCCAAAGCGTCCAAGAGAATCCCCTCCTCGGAGAAAACTTCCTCCCTCACCACGAAGAAGATCTCCTGTGCCTCGAAGAGCTGGATCTCCAAGACGACCAGAATCTCCTCGACGCCGTGCAGATTCTCCTGTCCGTCGTCGATTAGATTCTCCTTATCGCCGTGGAGCTGGAGACACACCTCCTAGGAGGAGACCTGTGTCTCCTGGTAGAGGTCGTTCTCCATCTCCCCCACCTAGGCGCTTGAGATCCCCTGCAAGAGTCTCACCTCGAAGAGTGCGTGGAAGCCCAGGCCCAGGTCGGAGACGTACTCCTCCTCCACCTCCAAGACGCCGTTCACCTCCCAGAAGAGCTCGTAGTCCTCCTAGAAGATCTCCAGTTGGTCGTAGGCGTAGTCGCTCTCCAATACGTAGATCTGCACGCTCTCGCTCAAGATCGTTCTCACCTCGCCGAGGTCGGCCACCTGTGAGACGGGGAAGATCATCATCTTACTCTGATTCACCAAGTCCTCGTAAGGTATCCCGGAGATCAAAGAGTCGAAGTCCAAGAAGGCCTTTGAAAGGAAGAGCTAGTAGCAACAGTAGCAGCAGCAGCTCACCACCTCCAGCCCGCAAGCCATAGATAATTCCTGTATATCTGTTTCCTCTGATATATTTATAAGGATTCTGTACTAGCAACTAAGAGTTATTCTAGCTGTAAGGGTGCTTGGGGGTATTTGTTGCATCTTATGGTTGTCTATTGTCTAATACATATTAGGGATTCAAGTAGTTTGAGAACTCAGTTACATTATTCTGCGTTTGGTTTTTGACTTTAGCAATGCTGTTTTTCTTTGTAATACAAATTTGGAAATGGTATTTTTATCCCTTTGCATAACCAGTTACAA
TARGET=1348,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04766 Ps_contig_mira-and-tgicl-ass_franssen_34570
SEQUENCE=AAAGAAATTCNTNTYTACCCCAATATCAGTCATCGAATTCAACTGTGATTGAACAGATTGAAATGTAATACAAAGGTCTTATCTGTTCAACCTCTAATTCAATTCTATGCTAAAAAGTTTGCTGTTGAATTGAGGTTTCCCGAATGGTTTCTCGACCACATGAGTTGGAGCCAAACTAAACGATTTCTGCAAAATCTTGTTTGTGTTCTTCTGCATGCTCCCATTTGAGGTTGAAACAAACATTCCTAGAAAGGGCTTATAAATAAATAATGAAACAATTAAGTACTAACTAAATGCCTCATGACACTGCTAAATTTTCTATTCTGGTTGCACAAATCACCACAAGGAAGGTACATAAACTTGTTCTTTCAGATTCTGAGTAGCACTTTCAGTAGGAAAACCTCTAACTCCACCAATAGTCCATTGTGATATAATTCCGGCTGCAATGGCAAAACGTAGCTGTCTGTCCAAACCATCTTGATTTTCAAACATTTCCGGACAAGTGGTTATTTTTCTCATAATAGCAGCCACAATCGCATCACCCGAGCCGGTTCTATCGCAAGTATACGGAGTTATGAGAACATCTTCTGTTCCTACCACAACCCCGTCAAAAGAAGGGGTATAATAGTGGATTCTAAATGTCCCATCAGTCACAAACAGGAATTTAAGTTTATCGTGCCACAAAGGCGAGATTTCCTCGGCAGTGTAATGGTAATACTCTTGACGATTCTTTGTTTGTTCATAATCTTCGGCATAGTATTGTGGCTTACGATTTCTCTTCCTCACATAATGTTCTTCGTCTAGAAGGAATTCTAACTCGGTTCTTGAAACTTCAATGATGTCTGCTGCTTGCCATGCTTTTTTTATTATCTGCCGAGTCTCGTCGCGTGATCTCCACAGAGGTAATGGCAAGTTTAAATCAAAAAACACAAGACCACCGAACTTTTTGAACCACTTAAGTGCTTTGAATAAAGTTGATTCCATAGATGGAGATGTTAGAACTTCTGCATTGAAATGGAAAATCCTAGCCTCTTTCAAAATAGAAAGATTCAATTCATCACTCCGAAGAGAATCCTCAGCAGCTTCCTTAACAGTCTCCATTCTCATCTTCCCACCATCAAACTTCACCTTCATATAACTACACCCAGTTCTAAATCCCGAATCAAATTTCACACCTCTAGTCTGCACCTTTTCCTTGTTCAAACCCATCACAATCTCTTCCCCAAACTCATCTTCCCCAACTTTCCCCAACAAAGCCGCCCGCCCGCCCAGCCTAACATGAGCCACCGCGACATTCGACGACGGCCCGCCAGGTGCTCGAGCAAATTCCGGAGGCTTCCATTGAAGCATTTTCCACTCCGAGTAAATGTCAGGATCCATCGGAAACTCGTGCACACGCACCGTAGGGAGAAACTCCCTCTGCGCTGCTCCGAAACAGCATACGAGCGGTGGTTCTTCGTATGGGAAATCGATTGCGTCGTCGTAGTCCTCTAATTCTTCTTCGATTGTTTCTGCGTTTTGGGGTTGTGATGTGTCGTTTTGGGTTGGGTTGGTTTTTCTGTTGCGTCGTTTTGGTGTTTGGGATTGTAGTTGTGTTGTGGAGGCTTTGATTTTGTGTTGGCTTTTTTGGTGTTGAAAAGTTGTGAATTTGGAAAGTGAGTTTTTTGGAGGGTGAAAGAGAGGAAGAAGGAGAAGATGGTGGTGATGAAGAATTGATGATGACTAGTTTTTTTTTTTGGTTT
TARGET=259,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04767 Ps_contig_mira-and-tgicl-ass_franssen_34570
SEQUENCE=AAAGAAATTCNTNTYTACCCCAATATCAGTCATCGAATTCAACTGTGATTGAACAGATTGAAATGTAATACAAAGGTCTTATCTGTTCAACCTCTAATTCAATTCTATGCTAAAAAGTTTGCTGTTGAATTGAGGTTTCCCGAATGGTTTCTCGACCACATGAGTTGGAGCCAAACTAAACGATTTCTGCAAAATCTTGTTTGTGTTCTTCTGCATGCTCCCATTTGAGGTTGAAACAAACATTCCTAGAAAGGGCTTATAAATAAATAATGAAACAATTAAGTACTAACTAAATGCCTCATGACACTGCTAAATTTTCTATTCTGGTTGCACAAATCACCACAAGGAAGGTACATAAACTTGTTCTTTCAGATTCTGAGTAGCACTTTCAGTAGGAAAACCTCTAACTCCACCAATAGTCCATTGTGATATAATTCCGGCTGCAATGGCAAAACGTAGCTGTCTGTCCAAACCATCTTGATTTTCAAACATTTCCGGACAAGTGGTTATTTTTCTCATAATAGCAGCCACAATCGCATCACCCGAGCCGGTTCTATCGCAAGTATACGGAGTTATGAGAACATCTTCTGTTCCTACCACAACCCCGTCAAAAGAAGGGGTATAATAGTGGATTCTAAATGTCCCATCAGTCACAAACAGGAATTTAAGTTTATCGTGCCACAAAGGCGAGATTTCCTCGGCAGTGTAATGGTAATACTCTTGACGATTCTTTGTTTGTTCATAATCTTCGGCATAGTATTGTGGCTTACGATTTCTCTTCCTCACATAATGTTCTTCGTCTAGAAGGAATTCTAACTCGGTTCTTGAAACTTCAATGATGTCTGCTGCTTGCCATGCTTTTTTTATTATCTGCCGAGTCTCGTCGCGTGATCTCCACAGAGGTAATGGCAAGTTTAAATCAAAAAACACAAGACCACCGAACTTTTTGAACCACTTAAGTGCTTTGAATAAAGTTGATTCCATAGATGGAGATGTTAGAACTTCTGCATTGAAATGGAAAATCCTAGCCTCTTTCAAAATAGAAAGATTCAATTCATCACTCCGAAGAGAATCCTCAGCAGCTTCCTTAACAGTCTCCATTCTCATCTTCCCACCATCAAACTTCACCTTCATATAACTACACCCAGTTCTAAATCCCGAATCAAATTTCACACCTCTAGTCTGCACCTTTTCCTTGTTCAAACCCATCACAATCTCTTCCCCAAACTCATCTTCCCCAACTTTCCCCAACAAAGCCGCCCGCCCGCCCAGCCTAACATGAGCCACCGCGACATTCGACGACGGCCCGCCAGGTGCTCGAGCAAATTCCGGAGGCTTCCATTGAAGCATTTTCCACTCCGAGTAAATGTCAGGATCCATCGGAAACTCGTGCACACGCACCGTAGGGAGAAACTCCCTCTGCGCTGCTCCGAAACAGCATACGAGCGGTGGTTCTTCGTATGGGAAATCGATTGCGTCGTCGTAGTCCTCTAATTCTTCTTCGATTGTTTCTGCGTTTTGGGGTTGTGATGTGTCGTTTTGGGTTGGGTTGGTTTTTCTGTTGCGTCGTTTTGGTGTTTGGGATTGTAGTTGTGTTGTGGAGGCTTTGATTTTGTGTTGGCTTTTTTGGTGTTGAAAAGTTGTGAATTTGGAAAGTGAGTTTTTTGGAGGGTGAAAGAGAGGAAGAAGGAGAAGATGGTGGTGATGAAGAATTGATGATGACTAGTTTTTTTTTTTGGTTT
TARGET=1257,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04768 Ps_contig_mira-and-tgicl-ass_franssen_34574
SEQUENCE=GGGAGTGAGAGAGAGAGRAAAGAAGTTCAATTAAAGTTGAGTTAAAGTCAAGTTCCTGGGCAAACATACAAATTCCTGAGAAATTGAAGTGGGAATCTAAATCTGAAATGAGTAGGGTCATTTTGTTTTCGCTCTTCTTGTTTCTGGTCTCTCAAACAGCTCAAGTTCAAAGCTCTTACGCTGGTTCCGCTAGTTCCATCATCAATCCTTCTAAAGTCAAACAGATTTCATGGAATCCAAGAGCTTTTGTTTATGAAGGTTTTCTCACGGACTTGGAATGCGATCACTTGATTTCTTTAGCGAAATCGGAGCTCAAGAGATCTGCTGTTGCGGATAACCTTTCCGGAGATAGTCAGTTAAGTGATGTTCGAACAAGCTCTGGCATGTTCATTTCCAAAAACAAGGATCCTATTATTTCTGGTATTGAGGACAAGATTTCTGCTTGGACCTTTCTTCCTAAAGAAAACGGGGAAGACATACAAGTATTGAGATATGAGCATGGACAGAAATATGACCCACATTACGATTACTTTACTGATAAAGTTAATATAGTTCAGGGAGGACATCGTCTCGCCACTGTTCTCATGTATCTTACTAATGTGACCAAAGGTGGTGAANYGTATTCCCAGAGGCTGAAAGGAAAACCTAACGAACGTACGACGAAGGGGTTACGTAGAAAAAAAAGTAAATGATCTCTCTGAGTGTGAC
TARGET=7,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04769 Ps_contig_mira-and-tgicl-ass_franssen_34577
SEQUENCE=AATTNAATNAAACAAAAATTTAAACTTTNYTTTCTTTNNTACAAAAAAATTTAAACGTTAAATACTGTAACACAGGGTAATGGTAAACTGTTTCTATCTTTGATAACAGAAAGAAGAAGAAGAGGATGAACAGTAATTATAGTTTAACAGAAACGATCAGCATCAGTGTTGTTAGACGGTGGTATCTTGAAGTGCCATAACCTTCCAACACCCTTCACCATGTTCTTCTTACAAAGAAACTCTTCAGCATAAGCCTTCTCCACCTTACGATCCACATCATGCAGAAAAACATGCGTCACACCAGATCCCTTTCTGTTTCTCGCCATAACAGCAGCGGAGAACACGGCGGCCATACGTCCCGGAGCCTCCGCGTAGTACCCTTTAGGCGCGTCTAGCATAATAAGGTCCCACTCCGTATCGTAAACTTCATCCGGTAAATTCTCAAGCGCGAGTTTACAAGCTTTGTTGCCACGTAGGAAGGTTTTAGAAGGAGAGCAAGTGGGCTCGGAGCGGTAAGATGTGATGAGCTTGTGGGCTTCCCGGAGCTGTGTCCGGTAACGAACTGTATGAGCCCGAAGGCCCGGTGCGTCTTTGAGAACGGCTTGAACCCATCGAGGATCTTCTTCAAGGAAGAGCGTGTTGCCACCTGGATTAAACGAGGCCCACATGAGTGAATCGTGACCGAGTCCAAATACCAGAAAGTTGCAGGGACGATTATAGGATTTCAATACATCGAAACTGATCTTGATCTCGGAGACAGATTGTTGAGGAACGACGCGTGATGTGGCGTAGTGGAGTATTGATTGTAGCTGGATCGGGGTTGGGTTGTTGTTGTCGTCGTTTGATCTGGTTCGGATTCCGGTTGAAATTGGACAGTGTAATGATGATGTGTCGGAGGCGGTGATTGCGGTGGCGATGAATAGTGCTGCTCCGATTAATCCAACGGTTGCTAATCCGATGAATAGCTTTCTCTGTTGGAGTGGATAACGGTTCTTCATCTTTTTAAGGTTTTTTTTGGTGTTGTTAATGTATTAATAATGCGGTTGTGTTGATGTGTTTGTGTAACATGTTTGTGTTGGGATTTGCTTGAAAGTTAAGAGAAGGTGATGATGAAGGTGGTTTGATTTTGTCAAATGATAATGATGGATTGGATGGGAATGAGGAAAATTGGTAGAGCAAGAGAACGAGTGAGAGTGAGAGGGTTCAATGGCAAAGAGATTAGGGAATGGAATGTTAGTGATTCTTTTTTAAGCCATTGTTTTTGAAGGAATTTGGGATTTTAATATCCAAATTGTTGGGGCCTCGTTGGGATGGGGATGGTGGTGAG
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04770 Ps_contig_mira-and-tgicl-ass_franssen_34579
SEQUENCE=CCACAGCTTTTCGAGCCACGTAGTCTGCGATATTCGCACTACAGCTCACTCCAAGAAGAACACATCTTGTTGGATCTCCATGACTAGCCAACCAAGGCTCAACCACTGAAGCACCAAAAGAATCAACAATATGCCTATGATTATCGGATTTGTTAAACTCACCACCACCACCACCACCACCACCGCCATGGCTTCCTCCACCAACCCTCCCAACCCCCCATAGATTTACTACACTCCGCCAAATTTGCCTGCTTCGCCAACCAATTCAACACCTTCAACCCATCCTCAAACGCTGCCGGATACCGACTCTCTGGCGCCAACCTATACCCAACAGCAACCACAATCACATCACAAAGCTTCGCGATCCGCCGACAAAACAAATCATTCGCCACCGAATCATTACTCCCACCTAACCCAACCCACCCCCACTGAAA
TARGET=160,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0772
PRIMER_LEFT_PENALTY=0.039879
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=ACCACTGAAGCACCAAAAGAAT
PRIMER_RIGHT_SEQUENCE=AAGCTTTGTGATGTGATTGTGG
PRIMER_LEFT=101,22
PRIMER_RIGHT=358,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.1202
PRIMER_LEFT_1_PENALTY=0.039879
PRIMER_RIGHT_1_PENALTY=0.080323
PRIMER_LEFT_1_SEQUENCE=ACCACTGAAGCACCAAAAGAAT
PRIMER_RIGHT_1_SEQUENCE=TTGTGGTTGCTGTTGGGTATAG
PRIMER_LEFT_1=101,22
PRIMER_RIGHT_1=342,22
PRIMER_LEFT_1_TM=60.040
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=4.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.1354
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.095523
PRIMER_LEFT_2_SEQUENCE=ACCACTGAAGCACCAAAAGAAT
PRIMER_RIGHT_2_SEQUENCE=GCTTTGTGATGTGATTGTGGTT
PRIMER_LEFT_2=101,22
PRIMER_RIGHT_2=356,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=59.904
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.1702
PRIMER_LEFT_3_PENALTY=0.039879
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=ACCACTGAAGCACCAAAAGAAT
PRIMER_RIGHT_3_SEQUENCE=GGTTGAAGGTGTTGAATTGGTT
PRIMER_LEFT_3=101,22
PRIMER_RIGHT_3=279,22
PRIMER_LEFT_3_TM=60.040
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=179
PRIMER_PAIR_PENALTY_4=0.1978
PRIMER_LEFT_4_PENALTY=0.067430
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=CGATATTCGCACTACAGCTCAC
PRIMER_RIGHT_4_SEQUENCE=GGTTGAAGGTGTTGAATTGGTT
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=279,22
PRIMER_LEFT_4_TM=59.933
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_04771 Ps_contig_mira-and-tgicl-ass_franssen_34596
SEQUENCE=GAAGACAAAAACAAAAAATCAAAATAAGCAATAAATCCCACTTTCTTTTGTTTAACTTTGAGATTCAATTTCACATTCAAATCCGTTTACCAAATCCACCACCATCTTTGACTCTTTTGTACCGGGAAATTAAACAACCACTAAATCTGCATTAATTCTACAAACCATATCCATCAATTTCCATTTTGAAAATGCTGTAACAACATTAAATTAAGATTAAGATGACTCTAAAATGAGATTAGTTGCATCTGCTGCAGAAAGTGAGTGATTGTGTTCTCCTTCATAGGTTACCACCAACATAGATGCATCATCAAGTGCTCTCTCTACATGTTTTCTTGCTGGACAACCTCTCACACTACTGCACTTGTAGTATCCTCTTGGATGAGGTGAACCCTTAATAGGTTTCTGTCCATATTTTCGCCAAGAGTAATCATCTGGTGGAATATCAGCCATCTTCAAGCTGATAGCAGGAACCCTAACAACCCTCTTCAATCTTAATTTTCTACTTTTTTTGGAACAATGACAGCGGCTAGATGAACTACCACACTTTCCAGAACCCAGAGTTTCAGAGCTACACTTTCTTTTCATGGAAGAAGAAGACAGTGGTGGCTTACCAATAGATGAAACATGAGAAAGATTCGTGATCTGAAAAGCTCCAGCCGGCGACGAAGATGACGGTTGCTGTTTGTTGTCTGTGTCGCCGGTGAGTGAGGAAATGAATGAATTAGCAGCGGATGAGTATGAAAAGTTAAGGGTTTTCGATGAGTCGTTTCTCTTGATTATAGCGCGGTGAAGTGTTGTTGTTGTTGTTGGTGGTGGTGGTGGTGGAATCTGTTGAAGAGGTGTAGCGTTGAAGATTGTTCGTTCAGAAGGTTGAGTTTGAGGTAAAGGAGCTTTTCTGAAACGAGCGTGCCCGGTTCTGGTTTTTTCGAGAAGTGAAATAACACGTTTGAATTTGGAAACAGCGATGTCAGCGACGGCTTTGCAGTCTCTTTCAATTTCCATGGAAGTGTTGTTGTTGTTGTTGGGATTTGAAGGTGAAAAGGAAGATGAAGATGAAGAGGTTTGGTATTTATGGCGTGCTTCAGAGAGTAATTTGATGAGTTTCTCAACGCTTTCTAAGCCCGAAGCAGCTTCTTGGACAGCATTTTCTTCGGCTATGGAAGTGAAAGTTTTGGCTGTGTTGTAACCTGTCATGAGTTTTACTGCCATAGCTGGAGCAACAAAACCATCAAAGAAAAAGGAAAGAGGAGAGAGAAAGAAGAAACGGGTGAGAATCAAAGAGGGGAGAGAGAGTGTTGACAAAGAAAAGAAAAAGAGTTATGTTTATGACTTTATGTCATNGAAATGTTTGTTTTT
TARGET=796,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0529
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.052187
PRIMER_LEFT_SEQUENCE=TAAGGGTTTTCGATGAGTCGTT
PRIMER_RIGHT_SEQUENCE=TCTTCAACGCTACACCTCTTCA
PRIMER_LEFT=749,22
PRIMER_RIGHT=856,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=108
PRIMER_PAIR_PENALTY_1=0.0529
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=TTAAGGGTTTTCGATGAGTCGT
PRIMER_RIGHT_1_SEQUENCE=TCTTCAACGCTACACCTCTTCA
PRIMER_LEFT_1=748,22
PRIMER_RIGHT_1=856,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.0529
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.052187
PRIMER_LEFT_2_SEQUENCE=GTTAAGGGTTTTCGATGAGTCG
PRIMER_RIGHT_2_SEQUENCE=TCTTCAACGCTACACCTCTTCA
PRIMER_LEFT_2=747,22
PRIMER_RIGHT_2=856,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.052
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=110
PRIMER_PAIR_PENALTY_3=0.0591
PRIMER_LEFT_3_PENALTY=0.006945
PRIMER_RIGHT_3_PENALTY=0.052187
PRIMER_LEFT_3_SEQUENCE=TTCGATGAGTCGTTTCTCTTGA
PRIMER_RIGHT_3_SEQUENCE=TCTTCAACGCTACACCTCTTCA
PRIMER_LEFT_3=757,22
PRIMER_RIGHT_3=856,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=60.052
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=0.1024
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.101680
PRIMER_LEFT_4_SEQUENCE=TAAGGGTTTTCGATGAGTCGTT
PRIMER_RIGHT_4_SEQUENCE=AATTGAAAGAGACTGCAAAGCC
PRIMER_LEFT_4=749,22
PRIMER_RIGHT_4=999,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_04772 Ps_contig_mira-and-tgicl-ass_franssen_34596
SEQUENCE=GAAGACAAAAACAAAAAATCAAAATAAGCAATAAATCCCACTTTCTTTTGTTTAACTTTGAGATTCAATTTCACATTCAAATCCGTTTACCAAATCCACCACCATCTTTGACTCTTTTGTACCGGGAAATTAAACAACCACTAAATCTGCATTAATTCTACAAACCATATCCATCAATTTCCATTTTGAAAATGCTGTAACAACATTAAATTAAGATTAAGATGACTCTAAAATGAGATTAGTTGCATCTGCTGCAGAAAGTGAGTGATTGTGTTCTCCTTCATAGGTTACCACCAACATAGATGCATCATCAAGTGCTCTCTCTACATGTTTTCTTGCTGGACAACCTCTCACACTACTGCACTTGTAGTATCCTCTTGGATGAGGTGAACCCTTAATAGGTTTCTGTCCATATTTTCGCCAAGAGTAATCATCTGGTGGAATATCAGCCATCTTCAAGCTGATAGCAGGAACCCTAACAACCCTCTTCAATCTTAATTTTCTACTTTTTTTGGAACAATGACAGCGGCTAGATGAACTACCACACTTTCCAGAACCCAGAGTTTCAGAGCTACACTTTCTTTTCATGGAAGAAGAAGACAGTGGTGGCTTACCAATAGATGAAACATGAGAAAGATTCGTGATCTGAAAAGCTCCAGCCGGCGACGAAGATGACGGTTGCTGTTTGTTGTCTGTGTCGCCGGTGAGTGAGGAAATGAATGAATTAGCAGCGGATGAGTATGAAAAGTTAAGGGTTTTCGATGAGTCGTTTCTCTTGATTATAGCGCGGTGAAGTGTTGTTGTTGTTGTTGGTGGTGGTGGTGGTGGAATCTGTTGAAGAGGTGTAGCGTTGAAGATTGTTCGTTCAGAAGGTTGAGTTTGAGGTAAAGGAGCTTTTCTGAAACGAGCGTGCCCGGTTCTGGTTTTTTCGAGAAGTGAAATAACACGTTTGAATTTGGAAACAGCGATGTCAGCGACGGCTTTGCAGTCTCTTTCAATTTCCATGGAAGTGTTGTTGTTGTTGTTGGGATTTGAAGGTGAAAAGGAAGATGAAGATGAAGAGGTTTGGTATTTATGGCGTGCTTCAGAGAGTAATTTGATGAGTTTCTCAACGCTTTCTAAGCCCGAAGCAGCTTCTTGGACAGCATTTTCTTCGGCTATGGAAGTGAAAGTTTTGGCTGTGTTGTAACCTGTCATGAGTTTTACTGCCATAGCTGGAGCAACAAAACCATCAAAGAAAAAGGAAAGAGGAGAGAGAAAGAAGAAACGGGTGAGAATCAAAGAGGGGAGAGAGAGTGTTGACAAAGAAAAGAAAAAGAGTTATGTTTATGACTTTATGTCATNGAAATGTTTGTTTTT
TARGET=811,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0529
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.052187
PRIMER_LEFT_SEQUENCE=TAAGGGTTTTCGATGAGTCGTT
PRIMER_RIGHT_SEQUENCE=TCTTCAACGCTACACCTCTTCA
PRIMER_LEFT=749,22
PRIMER_RIGHT=856,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=108
PRIMER_PAIR_PENALTY_1=0.0529
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=TTAAGGGTTTTCGATGAGTCGT
PRIMER_RIGHT_1_SEQUENCE=TCTTCAACGCTACACCTCTTCA
PRIMER_LEFT_1=748,22
PRIMER_RIGHT_1=856,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.0529
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.052187
PRIMER_LEFT_2_SEQUENCE=GTTAAGGGTTTTCGATGAGTCG
PRIMER_RIGHT_2_SEQUENCE=TCTTCAACGCTACACCTCTTCA
PRIMER_LEFT_2=747,22
PRIMER_RIGHT_2=856,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.052
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=110
PRIMER_PAIR_PENALTY_3=0.0591
PRIMER_LEFT_3_PENALTY=0.006945
PRIMER_RIGHT_3_PENALTY=0.052187
PRIMER_LEFT_3_SEQUENCE=TTCGATGAGTCGTTTCTCTTGA
PRIMER_RIGHT_3_SEQUENCE=TCTTCAACGCTACACCTCTTCA
PRIMER_LEFT_3=757,22
PRIMER_RIGHT_3=856,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=60.052
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=0.1024
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.101680
PRIMER_LEFT_4_SEQUENCE=TAAGGGTTTTCGATGAGTCGTT
PRIMER_RIGHT_4_SEQUENCE=AATTGAAAGAGACTGCAAAGCC
PRIMER_LEFT_4=749,22
PRIMER_RIGHT_4=999,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_04773 Ps_contig_mira-and-tgicl-ass_franssen_34596
SEQUENCE=GAAGACAAAAACAAAAAATCAAAATAAGCAATAAATCCCACTTTCTTTTGTTTAACTTTGAGATTCAATTTCACATTCAAATCCGTTTACCAAATCCACCACCATCTTTGACTCTTTTGTACCGGGAAATTAAACAACCACTAAATCTGCATTAATTCTACAAACCATATCCATCAATTTCCATTTTGAAAATGCTGTAACAACATTAAATTAAGATTAAGATGACTCTAAAATGAGATTAGTTGCATCTGCTGCAGAAAGTGAGTGATTGTGTTCTCCTTCATAGGTTACCACCAACATAGATGCATCATCAAGTGCTCTCTCTACATGTTTTCTTGCTGGACAACCTCTCACACTACTGCACTTGTAGTATCCTCTTGGATGAGGTGAACCCTTAATAGGTTTCTGTCCATATTTTCGCCAAGAGTAATCATCTGGTGGAATATCAGCCATCTTCAAGCTGATAGCAGGAACCCTAACAACCCTCTTCAATCTTAATTTTCTACTTTTTTTGGAACAATGACAGCGGCTAGATGAACTACCACACTTTCCAGAACCCAGAGTTTCAGAGCTACACTTTCTTTTCATGGAAGAAGAAGACAGTGGTGGCTTACCAATAGATGAAACATGAGAAAGATTCGTGATCTGAAAAGCTCCAGCCGGCGACGAAGATGACGGTTGCTGTTTGTTGTCTGTGTCGCCGGTGAGTGAGGAAATGAATGAATTAGCAGCGGATGAGTATGAAAAGTTAAGGGTTTTCGATGAGTCGTTTCTCTTGATTATAGCGCGGTGAAGTGTTGTTGTTGTTGTTGGTGGTGGTGGTGGTGGAATCTGTTGAAGAGGTGTAGCGTTGAAGATTGTTCGTTCAGAAGGTTGAGTTTGAGGTAAAGGAGCTTTTCTGAAACGAGCGTGCCCGGTTCTGGTTTTTTCGAGAAGTGAAATAACACGTTTGAATTTGGAAACAGCGATGTCAGCGACGGCTTTGCAGTCTCTTTCAATTTCCATGGAAGTGTTGTTGTTGTTGTTGGGATTTGAAGGTGAAAAGGAAGATGAAGATGAAGAGGTTTGGTATTTATGGCGTGCTTCAGAGAGTAATTTGATGAGTTTCTCAACGCTTTCTAAGCCCGAAGCAGCTTCTTGGACAGCATTTTCTTCGGCTATGGAAGTGAAAGTTTTGGCTGTGTTGTAACCTGTCATGAGTTTTACTGCCATAGCTGGAGCAACAAAACCATCAAAGAAAAAGGAAAGAGGAGAGAGAAAGAAGAAACGGGTGAGAATCAAAGAGGGGAGAGAGAGTGTTGACAAAGAAAAGAAAAAGAGTTATGTTTATGACTTTATGTCATNGAAATGTTTGTTTTT
TARGET=1011,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0659
PRIMER_LEFT_PENALTY=0.026744
PRIMER_RIGHT_PENALTY=0.039178
PRIMER_LEFT_SEQUENCE=CTGTTGAAGAGGTGTAGCGTTG
PRIMER_RIGHT_SEQUENCE=TACTCTCTGAAGCACGCCATAA
PRIMER_LEFT=831,22
PRIMER_RIGHT=1093,22
PRIMER_LEFT_TM=59.973
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.0659
PRIMER_LEFT_1_PENALTY=0.026744
PRIMER_RIGHT_1_PENALTY=0.039178
PRIMER_LEFT_1_SEQUENCE=CTGTTGAAGAGGTGTAGCGTTG
PRIMER_RIGHT_1_SEQUENCE=TTACTCTCTGAAGCACGCCATA
PRIMER_LEFT_1=831,22
PRIMER_RIGHT_1=1094,22
PRIMER_LEFT_1_TM=59.973
PRIMER_RIGHT_1_TM=60.039
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.0914
PRIMER_LEFT_2_PENALTY=0.052187
PRIMER_RIGHT_2_PENALTY=0.039178
PRIMER_LEFT_2_SEQUENCE=TGAAGAGGTGTAGCGTTGAAGA
PRIMER_RIGHT_2_SEQUENCE=TACTCTCTGAAGCACGCCATAA
PRIMER_LEFT_2=835,22
PRIMER_RIGHT_2=1093,22
PRIMER_LEFT_2_TM=60.052
PRIMER_RIGHT_2_TM=60.039
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.0914
PRIMER_LEFT_3_PENALTY=0.052187
PRIMER_RIGHT_3_PENALTY=0.039178
PRIMER_LEFT_3_SEQUENCE=TGAAGAGGTGTAGCGTTGAAGA
PRIMER_RIGHT_3_SEQUENCE=TTACTCTCTGAAGCACGCCATA
PRIMER_LEFT_3=835,22
PRIMER_RIGHT_3=1094,22
PRIMER_LEFT_3_TM=60.052
PRIMER_RIGHT_3_TM=60.039
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.1076
PRIMER_LEFT_4_PENALTY=0.026744
PRIMER_RIGHT_4_PENALTY=0.080887
PRIMER_LEFT_4_SEQUENCE=CTGTTGAAGAGGTGTAGCGTTG
PRIMER_RIGHT_4_SEQUENCE=AAATTACTCTCTGAAGCACGCC
PRIMER_LEFT_4=831,22
PRIMER_RIGHT_4=1097,22
PRIMER_LEFT_4_TM=59.973
PRIMER_RIGHT_4_TM=59.919
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_04774 Ps_contig_mira-and-tgicl-ass_franssen_34603
SEQUENCE=GGTTAAAATTNTACAAACATGGTAAGTTTCCAGGGTCTGATACTAAATATTAACAGCTCAACTCAAAATTTGCACAAGAGTACAACAAAGAAATAATTAATATTACATGACAACTAACCACTATTACTTTTCTTGTGTAAAATAGTACAACATGAAGGCATAATATTACAATCCTGCTGCATCCATGTCATCATTTGAAGCATTATCAATCTCCAGTTTAGCAGAAATTACCCTGTCCAGAGTTTCGTCTGAAACCGAGATCCCCGTTGTCTCCAAGTCTGCCACAACGGCAACGGCGGCATCTAGTTCCCCATGATCAAGGCAAAGAGATATGATCTCATCATAAAGCGGTCTCCCAAACTTATAGCCAAGGCCATGAGTTGTCTGCAAAATTGTCAAGAAAGCTGAAGGTAGAGGTGCTTTTATAGCTGCACGCAAGATCACGGCACAATCTCCAATAGTTGGCATCCCTCCCAATTGTATGACCTTCTGCATAACTTGGATTGCCAACTCGACTTCCCATTCCTGTGACCATCTATGAGGAGGCTTGCTCTTTAGTTCTCTCTCCCATGTCCATCCCCAAGCATCTGCTAAGGAGTACATGTCTGATACATCAAACACTCTACGATTCCTCATTTCCTTAAATGCCTCAAATATGTCCAAAGGAAACCAATCATCATCTGCATCATCCTCAACCATATTCCGAGTTGTTCTTCTTCTTGACTTTTTAGAAGAGGCAGAGGGTTGGTCGGAATCTTTCAACAGCTGGACTCCAATCATTTGAAGGGGCTTTTTAGCTTCAACTTCCTTTTCCTTTATCCTCTCTGCATCTTGGTTCTCTACTGGTTCTACTTCCTCTTCTGCCTGTTCTACTTCCTGATCATCATCATCTGCTTCATCATCTTCGGTCTCTTTTGCATCATCCTCTACAACATCAATATAATCTTGAACATCAGTGGACTCTGATGTACCTTCAATCATGGCATTTCCATGAGCACCAGGTAAGCCTTCTCCTAAGAAGCGGCGTTTCCAAAACTCTGTATTTCCTTCCTCCAGCTTTATGCGTGAAATCAATGCATCCAACTCTTCATCCACCTCTTCTTCCTCCTCTTGAATGGGAGGAACCCAAAGCGGCCGACCACGAGATTGATTTATTCTCCTTGCTTTCTGAACACGCTGGTAAAGAACATTTCTGGTTCCATCAGTAGGCAAACCTTGTCCTTCTAGTTCTTCCTTCAATTCAAGCACAAGCATTTTACTAGCAGCCTTAGGTTTTAAAGCATTCTGCTCAGGCCCCTTTATGATTTTCTTTAGCCACTCAAGAACTCTAACATAATCAGATTCAGATACATCATCCCCTAAAGCTGAAATTCCTTCATAGTGCAACTTCTTCAGAAGCTTTATATGGTACACCTTCCTCTCTTCAAGGCATCTTTGTTTGAAGGAGGTCTCTATTGGATTTGAATATATAAATCCGTCAGCATCAGGATCTAGAGGTGTTTTACCACTTCGAGGCACCCACCGTTTGCGCTCTCCAGTAAGGCCACCTTCTTCAACATATCTAGCAATATAATCAATCTCATAACCAAGTTCGGCCTCCTTTTGTAAAGGTTCAATCCATGAGCTCACTAAGGTCCGGTATTTTCTGCTCGAAATCATTGCCCGTGGGGGAATTTGCTGATTATCTTTGGCCATAGCTTCTAAAGCTTCGAGCAAGTCAATAATCCTTCCTTCCCGACACAATGCTCGAAGGTACAATGAAAGAGGATCTCCGTAGTTTCCTTCATTATGTAGAACTTTTATACCCCCTTCAAAGTTTTTAAGTGCTCGGAAATGCCTGATAGCTTCTCGAACTACACAGTACTTGGTAAAACACTCCACTAACAGCGCATGGGTTTTCACATTTGGCTGTATGCGCTTATGATCTTCAACCATCATGCCAAGCAACTCAGCGACATCTTGTACTCTGTCATAAGAATCAGCTCTAGTATACGCTTGAATGACCCAATTGTATGTTTCTGTATCCGGCTTCATATAATCTTCTCCATATTCCATGTTCTCAAATGTTGTAAAAGCTA
TARGET=880,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1264
PRIMER_LEFT_PENALTY=0.016312
PRIMER_RIGHT_PENALTY=0.110131
PRIMER_LEFT_SEQUENCE=CCTCTCTGCATCTTGGTTCTCT
PRIMER_RIGHT_SEQUENCE=AAGAGGTGGATGAAGAGTTGGA
PRIMER_LEFT=819,22
PRIMER_RIGHT=1099,22
PRIMER_LEFT_TM=60.016
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.1264
PRIMER_LEFT_1_PENALTY=0.016312
PRIMER_RIGHT_1_PENALTY=0.110131
PRIMER_LEFT_1_SEQUENCE=CCTCTCTGCATCTTGGTTCTCT
PRIMER_RIGHT_1_SEQUENCE=GAAGAGGTGGATGAAGAGTTGG
PRIMER_LEFT_1=819,22
PRIMER_RIGHT_1=1100,22
PRIMER_LEFT_1_TM=60.016
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=0.2266
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.222249
PRIMER_LEFT_2_SEQUENCE=AACAGCTGGACTCCAATCATTT
PRIMER_RIGHT_2_SEQUENCE=TTAGGAGAAGGCTTACCTGGTG
PRIMER_LEFT_2=760,22
PRIMER_RIGHT_2=1016,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=59.778
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.2386
PRIMER_LEFT_3_PENALTY=0.016312
PRIMER_RIGHT_3_PENALTY=0.222249
PRIMER_LEFT_3_SEQUENCE=CCTCTCTGCATCTTGGTTCTCT
PRIMER_RIGHT_3_SEQUENCE=TTAGGAGAAGGCTTACCTGGTG
PRIMER_LEFT_3=819,22
PRIMER_RIGHT_3=1016,22
PRIMER_LEFT_3_TM=60.016
PRIMER_RIGHT_3_TM=59.778
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=0.2592
PRIMER_LEFT_4_PENALTY=0.113803
PRIMER_RIGHT_4_PENALTY=0.145354
PRIMER_LEFT_4_SEQUENCE=TTTTAGAAGAGGCAGAGGGTTG
PRIMER_RIGHT_4_SEQUENCE=AAGAGACCGAAGATGATGAAGC
PRIMER_LEFT_4=725,22
PRIMER_RIGHT_4=913,22
PRIMER_LEFT_4_TM=59.886
PRIMER_RIGHT_4_TM=59.855
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_04775 Ps_contig_mira-and-tgicl-ass_franssen_34609
SEQUENCE=GTCGGGGGCCACACCCTGTTTCGGTGAACCACAGTTCATCAAGATCTCTCTCTCTTTCTCTCTCTAACACACATTCTCTCTCTCTAGAATCGAAGATGGGAAAGGGACCGGGTCTGTACTCCGATATCGGCAAGTTAGCCAAAGATCTTTTGACAAAGGATTACAACTCCGATCAGAAACTCACCGTTAAATCTTTCAGCACCACTGGAGTCGCCCTCACTTCAACTGCTCTAGAAAAAAGGAGGACTCTCAACTGGAGATGTGGACAGACAACTGTACAAGTATAAGAACACCCTTAATTAGATGTCAAACTTGACACAGCAT
TARGET=45,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1275
PRIMER_LEFT_PENALTY=0.012470
PRIMER_RIGHT_PENALTY=0.115007
PRIMER_LEFT_SEQUENCE=GGTGAACCACAGTTCATCAAGA
PRIMER_RIGHT_SEQUENCE=ATCTTTGGCTAACTTGCCGATA
PRIMER_LEFT=22,22
PRIMER_RIGHT=145,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.115
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=0.1712
PRIMER_LEFT_1_PENALTY=0.012470
PRIMER_RIGHT_1_PENALTY=0.158723
PRIMER_LEFT_1_SEQUENCE=GGTGAACCACAGTTCATCAAGA
PRIMER_RIGHT_1_SEQUENCE=GTCTGTCCACATCTCCAGTTGA
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=270,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=60.159
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=0.1870
PRIMER_LEFT_2_PENALTY=0.012470
PRIMER_RIGHT_2_PENALTY=0.174518
PRIMER_LEFT_2_SEQUENCE=GGTGAACCACAGTTCATCAAGA
PRIMER_RIGHT_2_SEQUENCE=ACTCCAGTGGTGCTGAAAGATT
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=210,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.175
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.2647
PRIMER_LEFT_3_PENALTY=0.012470
PRIMER_RIGHT_3_PENALTY=0.252279
PRIMER_LEFT_3_SEQUENCE=GGTGAACCACAGTTCATCAAGA
PRIMER_RIGHT_3_SEQUENCE=CATCTCCAGTTGAGAGTCCTCC
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=60.252
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=0.2660
PRIMER_LEFT_4_PENALTY=0.012470
PRIMER_RIGHT_4_PENALTY=0.253482
PRIMER_LEFT_4_SEQUENCE=GGTGAACCACAGTTCATCAAGA
PRIMER_RIGHT_4_SEQUENCE=TCCAGTGGTGCTGAAAGATTTA
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=208,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.747
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_04776 Ps_contig_mira-and-tgicl-ass_franssen_34609
SEQUENCE=GTCGGGGGCCACACCCTGTTTCGGTGAACCACAGTTCATCAAGATCTCTCTCTCTTTCTCTCTCTAACACACATTCTCTCTCTCTAGAATCGAAGATGGGAAAGGGACCGGGTCTGTACTCCGATATCGGCAAGTTAGCCAAAGATCTTTTGACAAAGGATTACAACTCCGATCAGAAACTCACCGTTAAATCTTTCAGCACCACTGGAGTCGCCCTCACTTCAACTGCTCTAGAAAAAAGGAGGACTCTCAACTGGAGATGTGGACAGACAACTGTACAAGTATAAGAACACCCTTAATTAGATGTCAAACTTGACACAGCAT
TARGET=75,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1275
PRIMER_LEFT_PENALTY=0.012470
PRIMER_RIGHT_PENALTY=0.115007
PRIMER_LEFT_SEQUENCE=GGTGAACCACAGTTCATCAAGA
PRIMER_RIGHT_SEQUENCE=ATCTTTGGCTAACTTGCCGATA
PRIMER_LEFT=22,22
PRIMER_RIGHT=145,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.115
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=0.1712
PRIMER_LEFT_1_PENALTY=0.012470
PRIMER_RIGHT_1_PENALTY=0.158723
PRIMER_LEFT_1_SEQUENCE=GGTGAACCACAGTTCATCAAGA
PRIMER_RIGHT_1_SEQUENCE=GTCTGTCCACATCTCCAGTTGA
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=270,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=60.159
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=0.1870
PRIMER_LEFT_2_PENALTY=0.012470
PRIMER_RIGHT_2_PENALTY=0.174518
PRIMER_LEFT_2_SEQUENCE=GGTGAACCACAGTTCATCAAGA
PRIMER_RIGHT_2_SEQUENCE=ACTCCAGTGGTGCTGAAAGATT
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=210,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.175
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.2647
PRIMER_LEFT_3_PENALTY=0.012470
PRIMER_RIGHT_3_PENALTY=0.252279
PRIMER_LEFT_3_SEQUENCE=GGTGAACCACAGTTCATCAAGA
PRIMER_RIGHT_3_SEQUENCE=CATCTCCAGTTGAGAGTCCTCC
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=261,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=60.252
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=0.2660
PRIMER_LEFT_4_PENALTY=0.012470
PRIMER_RIGHT_4_PENALTY=0.253482
PRIMER_LEFT_4_SEQUENCE=GGTGAACCACAGTTCATCAAGA
PRIMER_RIGHT_4_SEQUENCE=TCCAGTGGTGCTGAAAGATTTA
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=208,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.747
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_04777 Ps_contig_mira-and-tgicl-ass_franssen_34616
SEQUENCE=GAAACAACTTTAAGGTTAATTAGAATAAAGAATTTTTTTTTTACTTTANNTAGAAACAAAAACAAGAAAGCATCAACTTCAAATTATCATCATAATGGTGAAATTTCAACAATCATTCTCTCCTTGCAATTTCACTACTGCTCTTGAGGTAATCTGCTCTTATTGCCCATAATTGTTCACAAACTGCTTTCATGTCAGGTCTATCTGATCTTACTGGTGCTGCACAGCTAAATGCCAAATCTAACATCTTCATAACAATGTCGGTTTTTACAGCTTCTTGCATTAAAGGATCAAGCAGTTCCACCATATTTCCTTCATTATATTTCCTGAAGGCCCATCTTAATGTAACTCGCTCTTCAGGAGTTTTTTTCATTTCCACAGGACGGCGGCCAGTCAGAATTTCTAAAAGCAAAATTCCGAACGAGTAAACATCACTTTTGGGAGTGAGATGGTAGGTTTTCATATACTCAGGGTCCAAATAACCAACTGTTCCCTTCACTTTGGTAGAAATGTGTGTGTGGTCATTGCTTACAGGACCAAGCTTTGCAAATCCAAAATCTGCGACTTTAGCTCGCATCCTTTCAGTCAGAAGAATGTTGGATGATTTCACATCTCGATGGATAATTTGTTTTTCGGCATACAGATGCAAATAGGTCAATCCATGAGCAACATCAATTGCAATTTCAAGCCGCTGATTGAAATCCAGGATTTTTCCAAACAAACCATCCAAATGTTCTCGAAGAGTACCATTTGGCACAAACTCTGTAATAAGGATACGTTCGTTTCCTTTGTCAATATAACCAAGTAGTTTCACCAGGTTTCGATGATCAATTTTGGCCAGAAGTTCGACTTCACTGCTGAATTCTGTCCTCAAACTCTCAAAATGTTCCCTTTTTGCGCGTTTTACAGCCACGACCCGGCCATCATCTAAATTAGCCTTGTAGACAGTTCCAAAACCTCCCTCTCCTATTTGTAATGTTTCTGAGAAATTACGGGTGGCTTTAGAAACCTGACTCAGGTTGAGATGTAATGATTGAAGTCTACTGAGTTTCGGAGACATTGAGAATCTTGATGGACTTTGTGGCACCCGAAGTGGACTAGACGGGATCTTATCGGAAACAGATGGTTCAAAGGAGGAAGCTAAATCCATTGAATTTGAGTCCTTTTCCAGAACAGCATGAGCGGTTGTCTTCCTTCTCTTCCCATAGAAACAAGGACAAACAACAGCACAACATACCAACAAAGCTCCACCTACAAAAATTCCAACATGGTTGCTTGACACATGCTTAGAATCTCCTTGTGATGTGGAATTATTGTTCTAAACCTTTCTGACTAAACGTTTCCCTTCCC
TARGET=511,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0491
PRIMER_LEFT_PENALTY=0.008930
PRIMER_RIGHT_PENALTY=0.040146
PRIMER_LEFT_SEQUENCE=CTTTTGGGAGTGAGATGGTAGG
PRIMER_RIGHT_SEQUENCE=TGCTCATGGATTGACCTATTTG
PRIMER_LEFT=434,22
PRIMER_RIGHT=667,22
PRIMER_LEFT_TM=59.991
PRIMER_RIGHT_TM=59.960
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.0491
PRIMER_LEFT_1_PENALTY=0.008930
PRIMER_RIGHT_1_PENALTY=0.040146
PRIMER_LEFT_1_SEQUENCE=CTTTTGGGAGTGAGATGGTAGG
PRIMER_RIGHT_1_SEQUENCE=TGGATTGACCTATTTGCATCTG
PRIMER_LEFT_1=434,22
PRIMER_RIGHT_1=661,22
PRIMER_LEFT_1_TM=59.991
PRIMER_RIGHT_1_TM=59.960
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=0.0500
PRIMER_LEFT_2_PENALTY=0.009871
PRIMER_RIGHT_2_PENALTY=0.040146
PRIMER_LEFT_2_SEQUENCE=GCAAAATTCCGAACGAGTAAAC
PRIMER_RIGHT_2_SEQUENCE=TGCTCATGGATTGACCTATTTG
PRIMER_LEFT_2=408,22
PRIMER_RIGHT_2=667,22
PRIMER_LEFT_2_TM=60.010
PRIMER_RIGHT_2_TM=59.960
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.0500
PRIMER_LEFT_3_PENALTY=0.009871
PRIMER_RIGHT_3_PENALTY=0.040146
PRIMER_LEFT_3_SEQUENCE=GCAAAATTCCGAACGAGTAAAC
PRIMER_RIGHT_3_SEQUENCE=TGGATTGACCTATTTGCATCTG
PRIMER_LEFT_3=408,22
PRIMER_RIGHT_3=661,22
PRIMER_LEFT_3_TM=60.010
PRIMER_RIGHT_3_TM=59.960
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.0505
PRIMER_LEFT_4_PENALTY=0.008930
PRIMER_RIGHT_4_PENALTY=0.041553
PRIMER_LEFT_4_SEQUENCE=CTTTTGGGAGTGAGATGGTAGG
PRIMER_RIGHT_4_SEQUENCE=CTGAAAGGATGCGAGCTAAAGT
PRIMER_LEFT_4=434,22
PRIMER_RIGHT_4=584,22
PRIMER_LEFT_4_TM=59.991
PRIMER_RIGHT_4_TM=60.042
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_04778 Ps_contig_mira-and-tgicl-ass_franssen_34620
SEQUENCE=CCTTAAAACTAGAAGTTGGTACACCTTAAACGAAGTTTAATAACCTTTTTAAGGTTACGTTTTACCTAAATAATNTTCCCTACTTGTCTAAAACAAGACTGGCTTGTCTCCCGAGTCTTGGCTAATGGCTGTTGATGNTAAAAGACTTCAAGCTCGTGCTAGCTTTTTCTTTGAAGGAATGCTTCTCTTTGTTGTTGTTGTTGTCGGTGTTGTTATTGCAGCTTGAGCAGTACCTGGTTTGGTAAACCAAGTAGTATAGAGGAACTGTTTATGAGCGCCTACATGGTTGCAAACAAGACGAAGCTTCTGTCTCTCTCTCATCCATCTCAAAACTACTTTCATGTGATTCTTGCTCGTCAAATCTTTCAGTCCAACTTCCTTGACGCGTTCCCATGTGTTAGAAACGGTGATTGGACCATGCTCCTTGACGATATCAAAGAGAGCTCTGGTTATCGTTTGTCTCTGTTCCGGTGGCGTTGTCAGCGTCACCGCTCCCATCTTTGGCTTCAGTTTCTTTGCTGCATTTCTAATCGCCGTTCCAAACATTTCTCTACCCAAACCCTAATTTCTTACACCTCTGTTTTTTTTCAATTACAGTGTATTCTTACTTTAGCTCTGGACAGTGGAGATATTCCAGTCGTCTGTGCACTCATNNCGTTGRNNACCACYGTCCTTTCGTAGGAACGAACCGACCGACAAACGGAGGGGTAATACGGACGAACGGCCAACCGAACGGGAAGGGGGTAAGTAGG
TARGET=189,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04779 Ps_contig_mira-and-tgicl-ass_franssen_34620
SEQUENCE=CCTTAAAACTAGAAGTTGGTACACCTTAAACGAAGTTTAATAACCTTTTTAAGGTTACGTTTTACCTAAATAATNTTCCCTACTTGTCTAAAACAAGACTGGCTTGTCTCCCGAGTCTTGGCTAATGGCTGTTGATGNTAAAAGACTTCAAGCTCGTGCTAGCTTTTTCTTTGAAGGAATGCTTCTCTTTGTTGTTGTTGTTGTCGGTGTTGTTATTGCAGCTTGAGCAGTACCTGGTTTGGTAAACCAAGTAGTATAGAGGAACTGTTTATGAGCGCCTACATGGTTGCAAACAAGACGAAGCTTCTGTCTCTCTCTCATCCATCTCAAAACTACTTTCATGTGATTCTTGCTCGTCAAATCTTTCAGTCCAACTTCCTTGACGCGTTCCCATGTGTTAGAAACGGTGATTGGACCATGCTCCTTGACGATATCAAAGAGAGCTCTGGTTATCGTTTGTCTCTGTTCCGGTGGCGTTGTCAGCGTCACCGCTCCCATCTTTGGCTTCAGTTTCTTTGCTGCATTTCTAATCGCCGTTCCAAACATTTCTCTACCCAAACCCTAATTTCTTACACCTCTGTTTTTTTTCAATTACAGTGTATTCTTACTTTAGCTCTGGACAGTGGAGATATTCCAGTCGTCTGTGCACTCATNNCGTTGRNNACCACYGTCCTTTCGTAGGAACGAACCGACCGACAAACGGAGGGGTAATACGGACGAACGGCCAACCGAACGGGAAGGGGGTAAGTAGG
TARGET=310,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04780 Ps_contig_mira-and-tgicl-ass_franssen_34621
SEQUENCE=AGAGAGGCAGAGACAAAACAAGAGAAAACAAGAGAGAGAAAAATATAGAGTTTGTAAAGTTGCAATAACATTCACATTATGCTGATTTATTTCACACTAGAGCATATGAATTGAATTCAAAAGACAGTACTACTAAAGTAAACAAAGTGTGCTCTTTTCTTTTAACACACAAAACACAAACATGTTTGATGGAGTCCCAGACCAATTCCACCAATTCATAACTCCAAGAACCTCTTCATCATCACTACCTCTTCATTTACCTTTCCCTCTTTCAACAACACCAAATAACAACAACAACAACAACACTTTTCCATCTTTTGATCCTTATAATCATCATCAATTACCACCCCAACTTCAACCAAACAATCTCTTGCACCCATTACACCATCACACCAAAGATGAACAAAAGGAACAAACCAACACTCCTTCCATCAACAACTTCCAAATTGAAAGAGATCAAAGACAAATACTACCTCAACTCGTCGATCCTTGGACCAACGACGAAGTTCTTGCACTCTTGAAGATCAGATCCAACATGGAGAATTGGTTCCCAGATTTCACCTGGGAACACGTATCAAGGAAGCTAGCTGAGGTTGGATTTAAACGTAGTGCAGAGAAATGCAAAGAGAAATTTGAAGAAGAAAGTAGATTCTTCAACAATATTAATAATAATCAGAATAATTACGGTAAGAATTTTCGGTTTGTTACTGAACTTGAAGAGGTTTATCAAGGTGGTGGTGGTGGTGGTGAGAGTAAACGGAGCGAGGTGCAAGATAAGATGAGGAGTGGATCGAATGATGATGACGATGATTCGAGAAATGATGATGTTTTGGTGAAGAAGAAAGATGAAGATGAGGTGTTTGAGAAAAGTACTAGGAATGATGAGAGGAAGAGGAAAAGAGGTGGTGATAGATTTGAAGTGTTTAAGGGTTTTTGCGAGAGTGTTGTGAAGAAAATGATGGATCAACAAGAAGAGATGCATAACAAGCTTATTGAAGATATGATTAAAAGAGATGAAGAGAAGTTTAGTAGAGAAGAAGCTTGGAAGAAACAAGAGATGGAGAAAATGAACAAAGAGCTTGAGTTGATGGCACAAGAACAAGCTATTGCTGGTGATAGACAAGCCAATATAATTCAGTTCTTGAACAAATTCTCATCAACAACAAATTCCTCTTCAATTTTAGCTTCAATTCAAAGCTTTGGAATCGGAAAAGAAGACAAATCCAAGGTAATTGCACCTAAACATTTCTCGAATTCTACCACATTGCACTCACAAAACCCTAATCCTGAAATCCATGACAAAACTCTTCAACCAACATCTGAAAACCCTAGCTCAACCCTTCCTTCTTCTTCTACTACTTTGCCTCAACCTCGCACTTATACCGCCGGCGAGAAAGAGGATATTGGTAGAAGATGGCCAAAAGATGAAGTGTTGGCATTGATAAACTTGAGGTGCAATTACAACAACAATGAAGAAAAAGAAGGAAACAACAACAACAACACCAACAACACCAACAAGGCACCACTATGGGAGAGAATATCACAAGGGATGTTGGAGTTAGGATACAAAAGAAGTGCAAAGAGGTGCAAAGAGAAATGGGAGAACATAAACAAGTATTTCAGAAAAACAAAGGATAATGCTAATAGGAAAAGGTCACTTGATTCAAGAACTTGTCCTTATTTTCATCTATTGAGCAATCTTTATAATCAAGGTAAACTTGTTCTACAATCTGAAAAGAATGAAGGCAATAACAACAACATGAATCTGCCAGAAGAAAATGTTGTTCAAGAGAAACAAGCTCCACAAGATGAAAACAATGGTGGTGGTGAGTCTTCTGATCAAGTGGGGCCTGCATGCTGGTAGTTTCACTATATGTAGCAGGTTCATCATGGAGGTGAGAAAAAATTTATGCATGTACCTTCTTTGGATTTTGATAATCAGTTTTCATTTTCATTGATTTGTAGATTATCAATGACATATAATAAGTACATTAATTTTNNTTTAAAAAAAGT
TARGET=287,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0796
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.067436
PRIMER_LEFT_SEQUENCE=CTTTCCCTCTTTCAACAACACC
PRIMER_RIGHT_SEQUENCE=CAATTCTCCATGTTGGATCTGA
PRIMER_LEFT=260,22
PRIMER_RIGHT=545,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.0796
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.067436
PRIMER_LEFT_1_SEQUENCE=CCTTTCCCTCTTTCAACAACAC
PRIMER_RIGHT_1_SEQUENCE=CAATTCTCCATGTTGGATCTGA
PRIMER_LEFT_1=259,22
PRIMER_RIGHT_1=545,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.0796
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=0.067436
PRIMER_LEFT_2_SEQUENCE=ACCTTTCCCTCTTTCAACAACA
PRIMER_RIGHT_2_SEQUENCE=CAATTCTCCATGTTGGATCTGA
PRIMER_LEFT_2=258,22
PRIMER_RIGHT_2=545,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=59.933
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.0796
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=0.067436
PRIMER_LEFT_3_SEQUENCE=CTTTCCCTCTTTCAACAACACC
PRIMER_RIGHT_3_SEQUENCE=CCATGTTGGATCTGATCTTCAA
PRIMER_LEFT_3=260,22
PRIMER_RIGHT_3=538,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=59.933
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.0796
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=0.067436
PRIMER_LEFT_4_SEQUENCE=CCTTTCCCTCTTTCAACAACAC
PRIMER_RIGHT_4_SEQUENCE=CCATGTTGGATCTGATCTTCAA
PRIMER_LEFT_4=259,22
PRIMER_RIGHT_4=538,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.933
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_04781 Ps_contig_mira-and-tgicl-ass_franssen_34621
SEQUENCE=AGAGAGGCAGAGACAAAACAAGAGAAAACAAGAGAGAGAAAAATATAGAGTTTGTAAAGTTGCAATAACATTCACATTATGCTGATTTATTTCACACTAGAGCATATGAATTGAATTCAAAAGACAGTACTACTAAAGTAAACAAAGTGTGCTCTTTTCTTTTAACACACAAAACACAAACATGTTTGATGGAGTCCCAGACCAATTCCACCAATTCATAACTCCAAGAACCTCTTCATCATCACTACCTCTTCATTTACCTTTCCCTCTTTCAACAACACCAAATAACAACAACAACAACAACACTTTTCCATCTTTTGATCCTTATAATCATCATCAATTACCACCCCAACTTCAACCAAACAATCTCTTGCACCCATTACACCATCACACCAAAGATGAACAAAAGGAACAAACCAACACTCCTTCCATCAACAACTTCCAAATTGAAAGAGATCAAAGACAAATACTACCTCAACTCGTCGATCCTTGGACCAACGACGAAGTTCTTGCACTCTTGAAGATCAGATCCAACATGGAGAATTGGTTCCCAGATTTCACCTGGGAACACGTATCAAGGAAGCTAGCTGAGGTTGGATTTAAACGTAGTGCAGAGAAATGCAAAGAGAAATTTGAAGAAGAAAGTAGATTCTTCAACAATATTAATAATAATCAGAATAATTACGGTAAGAATTTTCGGTTTGTTACTGAACTTGAAGAGGTTTATCAAGGTGGTGGTGGTGGTGGTGAGAGTAAACGGAGCGAGGTGCAAGATAAGATGAGGAGTGGATCGAATGATGATGACGATGATTCGAGAAATGATGATGTTTTGGTGAAGAAGAAAGATGAAGATGAGGTGTTTGAGAAAAGTACTAGGAATGATGAGAGGAAGAGGAAAAGAGGTGGTGATAGATTTGAAGTGTTTAAGGGTTTTTGCGAGAGTGTTGTGAAGAAAATGATGGATCAACAAGAAGAGATGCATAACAAGCTTATTGAAGATATGATTAAAAGAGATGAAGAGAAGTTTAGTAGAGAAGAAGCTTGGAAGAAACAAGAGATGGAGAAAATGAACAAAGAGCTTGAGTTGATGGCACAAGAACAAGCTATTGCTGGTGATAGACAAGCCAATATAATTCAGTTCTTGAACAAATTCTCATCAACAACAAATTCCTCTTCAATTTTAGCTTCAATTCAAAGCTTTGGAATCGGAAAAGAAGACAAATCCAAGGTAATTGCACCTAAACATTTCTCGAATTCTACCACATTGCACTCACAAAACCCTAATCCTGAAATCCATGACAAAACTCTTCAACCAACATCTGAAAACCCTAGCTCAACCCTTCCTTCTTCTTCTACTACTTTGCCTCAACCTCGCACTTATACCGCCGGCGAGAAAGAGGATATTGGTAGAAGATGGCCAAAAGATGAAGTGTTGGCATTGATAAACTTGAGGTGCAATTACAACAACAATGAAGAAAAAGAAGGAAACAACAACAACAACACCAACAACACCAACAAGGCACCACTATGGGAGAGAATATCACAAGGGATGTTGGAGTTAGGATACAAAAGAAGTGCAAAGAGGTGCAAAGAGAAATGGGAGAACATAAACAAGTATTTCAGAAAAACAAAGGATAATGCTAATAGGAAAAGGTCACTTGATTCAAGAACTTGTCCTTATTTTCATCTATTGAGCAATCTTTATAATCAAGGTAAACTTGTTCTACAATCTGAAAAGAATGAAGGCAATAACAACAACATGAATCTGCCAGAAGAAAATGTTGTTCAAGAGAAACAAGCTCCACAAGATGAAAACAATGGTGGTGGTGAGTCTTCTGATCAAGTGGGGCCTGCATGCTGGTAGTTTCACTATATGTAGCAGGTTCATCATGGAGGTGAGAAAAAATTTATGCATGTACCTTCTTTGGATTTTGATAATCAGTTTTCATTTTCATTGATTTGTAGATTATCAATGACATATAATAAGTACATTAATTTTNNTTTAAAAAAAGT
TARGET=731,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0958
PRIMER_LEFT_PENALTY=0.052366
PRIMER_RIGHT_PENALTY=0.043439
PRIMER_LEFT_SEQUENCE=ACGTAGTGCAGAGAAATGCAAA
PRIMER_RIGHT_SEQUENCE=TTCTCGAATCATCGTCATCATC
PRIMER_LEFT=603,22
PRIMER_RIGHT=817,22
PRIMER_LEFT_TM=59.948
PRIMER_RIGHT_TM=60.043
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=215
PRIMER_PAIR_PENALTY_1=0.0958
PRIMER_LEFT_1_PENALTY=0.052366
PRIMER_RIGHT_1_PENALTY=0.043439
PRIMER_LEFT_1_SEQUENCE=AACGTAGTGCAGAGAAATGCAA
PRIMER_RIGHT_1_SEQUENCE=TTCTCGAATCATCGTCATCATC
PRIMER_LEFT_1=602,22
PRIMER_RIGHT_1=817,22
PRIMER_LEFT_1_TM=59.948
PRIMER_RIGHT_1_TM=60.043
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=216
PRIMER_PAIR_PENALTY_2=0.1036
PRIMER_LEFT_2_PENALTY=0.060196
PRIMER_RIGHT_2_PENALTY=0.043439
PRIMER_LEFT_2_SEQUENCE=GATCCAACATGGAGAATTGGTT
PRIMER_RIGHT_2_SEQUENCE=TTCTCGAATCATCGTCATCATC
PRIMER_LEFT_2=527,22
PRIMER_RIGHT_2=817,22
PRIMER_LEFT_2_TM=60.060
PRIMER_RIGHT_2_TM=60.043
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.1036
PRIMER_LEFT_3_PENALTY=0.060196
PRIMER_RIGHT_3_PENALTY=0.043439
PRIMER_LEFT_3_SEQUENCE=ATCCAACATGGAGAATTGGTTC
PRIMER_RIGHT_3_SEQUENCE=TTCTCGAATCATCGTCATCATC
PRIMER_LEFT_3=528,22
PRIMER_RIGHT_3=817,22
PRIMER_LEFT_3_TM=60.060
PRIMER_RIGHT_3_TM=60.043
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.1180
PRIMER_LEFT_4_PENALTY=0.074581
PRIMER_RIGHT_4_PENALTY=0.043439
PRIMER_LEFT_4_SEQUENCE=CGTAGTGCAGAGAAATGCAAAG
PRIMER_RIGHT_4_SEQUENCE=TTCTCGAATCATCGTCATCATC
PRIMER_LEFT_4=604,22
PRIMER_RIGHT_4=817,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=60.043
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_04782 Ps_contig_mira-and-tgicl-ass_franssen_34621
SEQUENCE=AGAGAGGCAGAGACAAAACAAGAGAAAACAAGAGAGAGAAAAATATAGAGTTTGTAAAGTTGCAATAACATTCACATTATGCTGATTTATTTCACACTAGAGCATATGAATTGAATTCAAAAGACAGTACTACTAAAGTAAACAAAGTGTGCTCTTTTCTTTTAACACACAAAACACAAACATGTTTGATGGAGTCCCAGACCAATTCCACCAATTCATAACTCCAAGAACCTCTTCATCATCACTACCTCTTCATTTACCTTTCCCTCTTTCAACAACACCAAATAACAACAACAACAACAACACTTTTCCATCTTTTGATCCTTATAATCATCATCAATTACCACCCCAACTTCAACCAAACAATCTCTTGCACCCATTACACCATCACACCAAAGATGAACAAAAGGAACAAACCAACACTCCTTCCATCAACAACTTCCAAATTGAAAGAGATCAAAGACAAATACTACCTCAACTCGTCGATCCTTGGACCAACGACGAAGTTCTTGCACTCTTGAAGATCAGATCCAACATGGAGAATTGGTTCCCAGATTTCACCTGGGAACACGTATCAAGGAAGCTAGCTGAGGTTGGATTTAAACGTAGTGCAGAGAAATGCAAAGAGAAATTTGAAGAAGAAAGTAGATTCTTCAACAATATTAATAATAATCAGAATAATTACGGTAAGAATTTTCGGTTTGTTACTGAACTTGAAGAGGTTTATCAAGGTGGTGGTGGTGGTGGTGAGAGTAAACGGAGCGAGGTGCAAGATAAGATGAGGAGTGGATCGAATGATGATGACGATGATTCGAGAAATGATGATGTTTTGGTGAAGAAGAAAGATGAAGATGAGGTGTTTGAGAAAAGTACTAGGAATGATGAGAGGAAGAGGAAAAGAGGTGGTGATAGATTTGAAGTGTTTAAGGGTTTTTGCGAGAGTGTTGTGAAGAAAATGATGGATCAACAAGAAGAGATGCATAACAAGCTTATTGAAGATATGATTAAAAGAGATGAAGAGAAGTTTAGTAGAGAAGAAGCTTGGAAGAAACAAGAGATGGAGAAAATGAACAAAGAGCTTGAGTTGATGGCACAAGAACAAGCTATTGCTGGTGATAGACAAGCCAATATAATTCAGTTCTTGAACAAATTCTCATCAACAACAAATTCCTCTTCAATTTTAGCTTCAATTCAAAGCTTTGGAATCGGAAAAGAAGACAAATCCAAGGTAATTGCACCTAAACATTTCTCGAATTCTACCACATTGCACTCACAAAACCCTAATCCTGAAATCCATGACAAAACTCTTCAACCAACATCTGAAAACCCTAGCTCAACCCTTCCTTCTTCTTCTACTACTTTGCCTCAACCTCGCACTTATACCGCCGGCGAGAAAGAGGATATTGGTAGAAGATGGCCAAAAGATGAAGTGTTGGCATTGATAAACTTGAGGTGCAATTACAACAACAATGAAGAAAAAGAAGGAAACAACAACAACAACACCAACAACACCAACAAGGCACCACTATGGGAGAGAATATCACAAGGGATGTTGGAGTTAGGATACAAAAGAAGTGCAAAGAGGTGCAAAGAGAAATGGGAGAACATAAACAAGTATTTCAGAAAAACAAAGGATAATGCTAATAGGAAAAGGTCACTTGATTCAAGAACTTGTCCTTATTTTCATCTATTGAGCAATCTTTATAATCAAGGTAAACTTGTTCTACAATCTGAAAAGAATGAAGGCAATAACAACAACATGAATCTGCCAGAAGAAAATGTTGTTCAAGAGAAACAAGCTCCACAAGATGAAAACAATGGTGGTGGTGAGTCTTCTGATCAAGTGGGGCCTGCATGCTGGTAGTTTCACTATATGTAGCAGGTTCATCATGGAGGTGAGAAAAAATTTATGCATGTACCTTCTTTGGATTTTGATAATCAGTTTTCATTTTCATTGATTTGTAGATTATCAATGACATATAATAAGTACATTAATTTTNNTTTAAAAAAAGT
TARGET=1487,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1053
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.101680
PRIMER_LEFT_SEQUENCE=TGGCATTGATAAACTTGAGGTG
PRIMER_RIGHT_SEQUENCE=ATTTCTCTTTGCACCTCTTTGC
PRIMER_LEFT=1433,22
PRIMER_RIGHT=1597,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=0.1060
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=AGATGGCCAAAAGATGAAGTGT
PRIMER_RIGHT_1_SEQUENCE=ATTTCTCTTTGCACCTCTTTGC
PRIMER_LEFT_1=1411,22
PRIMER_RIGHT_1=1597,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.1202
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.116582
PRIMER_LEFT_2_SEQUENCE=TGGCATTGATAAACTTGAGGTG
PRIMER_RIGHT_2_SEQUENCE=GTTCTCCCATTTCTCTTTGCAC
PRIMER_LEFT_2=1433,22
PRIMER_RIGHT_2=1605,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.1396
PRIMER_LEFT_3_PENALTY=0.003589
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=TGGCATTGATAAACTTGAGGTG
PRIMER_RIGHT_3_SEQUENCE=TCCTAACTCCAACATCCCTTGT
PRIMER_LEFT_3=1433,22
PRIMER_RIGHT_3=1563,22
PRIMER_LEFT_3_TM=59.996
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=0.1404
PRIMER_LEFT_4_PENALTY=0.004359
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=AGATGGCCAAAAGATGAAGTGT
PRIMER_RIGHT_4_SEQUENCE=TCCTAACTCCAACATCCCTTGT
PRIMER_LEFT_4=1411,22
PRIMER_RIGHT_4=1563,22
PRIMER_LEFT_4_TM=60.004
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_04783 Ps_contig_mira-and-tgicl-ass_franssen_34626
SEQUENCE=TTTAGTAAAAAACTTTGAATTTTATTGCATATATACTATGTAAAACTTGAATGTGAATATGATGAGATCAAGAGTTTCTCTAAGGCGTTCCACAAAAAAGTTGATTACACATGCACATTCAAAACCTATTACTATAAGGTGTTCCATATACAATAAGGTATGGTACACTTCTGATTACACTGTCTATGGGAATTGCAAAGTTAACACCTGATGAAGCTCCACTCCCTTTCCTTGTGAAGGTGGCAGTGTTTACTCCAACAACATGGCCATGAGAGTCAATCAACGGTCCACCTGAATTTCCTGCATTAATAGCCGCATCTGTTTGAATAGCTCCTCTGATAGCCCCTCCGTTCGGGGAAGGTATCTCGCGGCCTAAACCACTGACCACCCCCGTTGTGAGAGTATTCTCATATCCATAAGGATTTCCAATTGCAAAGCAACTCTGCCCAACAAGTAAATTCTTAGATTCACCAAGAAGAACAGGCTTTATTTCGTATCCATCAACATCAACCTTCAGAACAGCTAGATCATATGATGGATCAAAACCAACTATTTTCCCTTCCCTTGAAAATCTATTCCCTTTGGCATCCACTAGGAAAACCTTACAACGTTGTAAACCACTTGTATCGGTAGCCAGTTTAGCTACAACATGGTAATTGGTAACAATGTGACCAAATTTATCCCAAACGAAGCCTGAACCAGTTCCTTCAACTTTCGCATCTTCATCGTCATCTAACACGGCTACATTAGAAGGGTTCTTGGGGTCTTTAGCCAATTCAATGTCTTGGATGGAAACAACTGATGGTGAAGTTTCCTGAAAAAGTTGCACAAGGTGGTCTTCTTGTTGCTGAAGTTCATCTTGAAGCTGCTGTGAATGTGATGAAGTGAGATTGCGGAGATTAAGCCATGAGGCAACTACGAGGCTTGAATTGAAGACTATTGCTCGTCGTTTGGTGACAGTAGCTGTAACAGTGGTGGTGGTGGGAAGGGTTTTTGTTGAGGATTGCGGTGTTAGCATTGGTGAGGGAAAGAGGTTCGTTTGGAGAGAAAACATTGTTATGGTTCCGATTATCCCACGCTTTTTTCTTCGGTTTCTGGTTGATTAGGGAGAAGACCATATAGCCATTCCTTTTTCTCGAATCTTTCT
TARGET=972,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0280
PRIMER_LEFT_PENALTY=0.022302
PRIMER_RIGHT_PENALTY=0.005674
PRIMER_LEFT_SEQUENCE=TCATCTTGAAGCTGCTGTGAAT
PRIMER_RIGHT_SEQUENCE=CCTAATCAACCAGAAACCGAAG
PRIMER_LEFT=854,22
PRIMER_RIGHT=1106,22
PRIMER_LEFT_TM=60.022
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.0805
PRIMER_LEFT_1_PENALTY=0.074807
PRIMER_RIGHT_1_PENALTY=0.005674
PRIMER_LEFT_1_SEQUENCE=AAAAAGTTGCACAAGGTGGTCT
PRIMER_RIGHT_1_SEQUENCE=CCTAATCAACCAGAAACCGAAG
PRIMER_LEFT_1=817,22
PRIMER_RIGHT_1=1106,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=0.0805
PRIMER_LEFT_2_PENALTY=0.074807
PRIMER_RIGHT_2_PENALTY=0.005674
PRIMER_LEFT_2_SEQUENCE=AAAAGTTGCACAAGGTGGTCTT
PRIMER_RIGHT_2_SEQUENCE=CCTAATCAACCAGAAACCGAAG
PRIMER_LEFT_2=818,22
PRIMER_RIGHT_2=1106,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.1070
PRIMER_LEFT_3_PENALTY=0.022302
PRIMER_RIGHT_3_PENALTY=0.084740
PRIMER_LEFT_3_SEQUENCE=TCATCTTGAAGCTGCTGTGAAT
PRIMER_RIGHT_3_SEQUENCE=GGGATAATCGGAACCATAACAA
PRIMER_LEFT_3=854,22
PRIMER_RIGHT_3=1074,22
PRIMER_LEFT_3_TM=60.022
PRIMER_RIGHT_3_TM=59.915
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.1083
PRIMER_LEFT_4_PENALTY=0.102638
PRIMER_RIGHT_4_PENALTY=0.005674
PRIMER_LEFT_4_SEQUENCE=ATGAAGTGAGATTGCGGAGATT
PRIMER_RIGHT_4_SEQUENCE=CCTAATCAACCAGAAACCGAAG
PRIMER_LEFT_4=879,22
PRIMER_RIGHT_4=1106,22
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_04784 Ps_contig_mira-and-tgicl-ass_franssen_34627
SEQUENCE=GTATACTAGTTAAGGTAAAAACCGTTAAAACCCCGGTTTTAACCCGGGTTTTAACCGTTTACCGTACGGTAACGGTGTCGTATACGTTTACGGTTAAGTACGTACGTACTTCCGACGTTTTTTCCGCGTTAATCGGCCATGGAGATCCGTGCCGATGCAGAAACTAGTTCGTTTTCTTCAAGTTTGTTTGCGGCAGTTTCGTATGGATTTGCTTCAATGGCGATGGTTTTTATCAATAAAGCTGTTCTTATGCAGTATTCGTATTCAATGACTTTACTCACTTTGCAGCAACTAGTTACTACTTTGCTTATACACTTTGGTCGAAAAATGGGATACACAAGAGCTAGAGGAGTGGATATGGAAACGGCCAAGCAACTGTTCCCGGTTTCATTTTTCTATAATGCCAATGTTGCATTTGCTTTGGCCAGTTTGAAAGGAGTCAACATCCCAATGTATATTGCAATAAAGAGGCTTACTCCGCTTGCTGTACTTATCGCCGGATGTTTCATGGGGAAGGGGAGACCTACAACTCAGGTGACTCTCTCAGTGATATTGACTGCTGCTGGAGTTCTAATAGCAGCCCTTGGAGATTTTTCCTTTGACCTTTTTGGATATAGCTTGGCTTTCATTTCTGTTTTCTTCCAGACTATGTACCTTGTGCTGGTGGAAAAATCTGGTGCTGAGAATGGACTTTCATCAGTGGAAATCATGTTCTACAACAGTTTTTTATCTCTTCCATTTTTGATGTTTCTTATTATAGCCACAGGGGAATTCCCATATTCTTTATCTGTATTATTTGCAAAGAGTTATTCTTTTTCGTTTTTGTTGATCCTTATTCTTTCATTGGTGATGGCCATTGTTCTCAACTTCACCATGTTCTTGTGCACTATTGTTAATTCTGCTTTAACTACAACTATTGTGGGCGTTCTTAAAGGAGTTGGTTCCACGACCTTTGGTTTCTTCTTACTGGGTGGTGTTCAAGTCCATGCTTTGAATGTATCTGGATTGGTTATCAATACGGCTGGTGGCGTGTGGTACTCATATGCTAAGTATCAACAGAAAAAAAGTAAGACAGTGAAGCTAGTTACAGACGTGGAGACTCATCGTAAATAGAAATGGTGTTGATGACTTACAGCGAAAACTAACAGTTGCATAAATGTTTCTTTTTCTTTAAATTGATATTCTAGTTGTGTATTTGTATTCGTAATTCTCTCTTTCTCTACYGTCTCCCATCATTTTACAGTATTCACAGAATCTCACACACATACACACATGTCGAAAAGGTA
TARGET=98,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04785 Ps_contig_mira-and-tgicl-ass_franssen_34631
SEQUENCE=AACGACACGTAGGTACTACAACGACAGAGTACGCGGGGGTAGTTATCAAGCAAGCAAGTCAACGAACCTATCAATGGGCTCAATTCTCTCTTCCCTCATCGGCGGCGAACCAGCTGCCGCCTTAGAATCCTCCGATCACACCGTCAAAACATTCCACTCATCCGCACGCTGGCAGCTCCACTTCAACGAAATCAAAGACTCGCCTCGTCTCGTTGTGATTGATTTCTCTGCTACGTGGTGTGGACCGTGTAAAATGATGGAACCGATTGTTCATGCCATGGCTAACGAATTCACGGACGTTGAATTCATCAAGATCGACGTTGACGAATTATCGGATGTGGCGCAGGAGTTTAAGGTGCAGGCTATGCCGACGTTTTTGTTGTTGAAGAATGGGGAAGAGGTGGATAAGATTGTTGGTGCCAAAAAAGATGAACTCAAAAACAAGATTAAAAAATACAGAGCTTAGTACTTATTTTATTTTCAACAATAAAGAGAAAATTGAACATGATGTTATTATGATGGTACAATTGTAAAATTTTAACTGGTTGAATGATGTTTATGTATTTGTTTGATATTGCATATGACTTGTGAATGTAATGTAAAACGGTTAATTGCAAAACTATTTTTTTTGTTGGTAGTTAGTTTTAGGTTACTAAGTTTATGTAGTGTAGAATAAAATACTAAAAGTTAAGTAAAAATTTAAAA
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7685
PRIMER_LEFT_PENALTY=0.730179
PRIMER_RIGHT_PENALTY=0.038361
PRIMER_LEFT_SEQUENCE=AGAGTACGCGGGGGTAGTTATC
PRIMER_RIGHT_SEQUENCE=GTGTGATCGGAGGATTCTAAGG
PRIMER_LEFT=25,22
PRIMER_RIGHT=140,22
PRIMER_LEFT_TM=60.730
PRIMER_RIGHT_TM=59.962
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=116
PRIMER_PAIR_PENALTY_1=0.7685
PRIMER_LEFT_1_PENALTY=0.730179
PRIMER_RIGHT_1_PENALTY=0.038361
PRIMER_LEFT_1_SEQUENCE=AGAGTACGCGGGGGTAGTTATC
PRIMER_RIGHT_1_SEQUENCE=GGTGTGATCGGAGGATTCTAAG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=141,22
PRIMER_LEFT_1_TM=60.730
PRIMER_RIGHT_1_TM=59.962
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.8480
PRIMER_LEFT_2_PENALTY=0.730179
PRIMER_RIGHT_2_PENALTY=0.117817
PRIMER_LEFT_2_SEQUENCE=AGAGTACGCGGGGGTAGTTATC
PRIMER_RIGHT_2_SEQUENCE=GAAATCAATCACAACGAGACGA
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=225,22
PRIMER_LEFT_2_TM=60.730
PRIMER_RIGHT_2_TM=60.118
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.8842
PRIMER_LEFT_3_PENALTY=0.730179
PRIMER_RIGHT_3_PENALTY=0.154023
PRIMER_LEFT_3_SEQUENCE=AGAGTACGCGGGGGTAGTTATC
PRIMER_RIGHT_3_SEQUENCE=CATCATTTTACACGGTCCACAC
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=258,22
PRIMER_LEFT_3_TM=60.730
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.9273
PRIMER_LEFT_4_PENALTY=0.730179
PRIMER_RIGHT_4_PENALTY=0.197140
PRIMER_LEFT_4_SEQUENCE=AGAGTACGCGGGGGTAGTTATC
PRIMER_RIGHT_4_SEQUENCE=ACACCACGTAGCAGAGAAATCA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=240,22
PRIMER_LEFT_4_TM=60.730
PRIMER_RIGHT_4_TM=59.803
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_04786 Ps_contig_mira-and-tgicl-ass_franssen_34639
SEQUENCE=AATCCATTTGTATTGTAGTAAGGTAAAACATCTCCCAGCATCAAATCCTATAGATAAGATCACTTTCAAAAACAGTATCATTATTCTTTGTTTCACCTTAAACATGTTACTTCCTTTTCCTATATAAACCCACTAACTAGCCCAGTAGTACTCTCATATTATCATAATATTCCATTATCATTTCTGAAGAAGCTAATTAGCATTGTTTCAGCACTGGTTCTCTCATCTCAACTTGTGTAGATTAAGTAGAAGAAAGGAGGGGAATTGAATATTCAACTTCATGGATGTTGCACACTTTTTGTTTGGCATATTTGGGAATGCTTCCGCTTTGTTCCTCTTCTTGGCACCGGTGATTACATTCAAGAGGATTATAGTGAACAAATCAACAGAGAAGTTCTCAGGCCTTCCATATGTTATGACACTGCTCAATTGTCTTCTTTCTGCTTGGTACGGTCTTCCTTTTGTGTCTCCACACAACATACTAGTATCAACAATAAATGGCACAGGAGCAGTAATTGAAATCATATATGTTTTGATATTCATCACATTTGCACCTAAGAAAGAAAAGATCAAAATCTTTGGCCTATTCACCGCGGTACTCTCCGTGTTCTCCGTTGTTGTTTTCGTGTCGCTCTTTGCCCTACACGGCACGCCTAGGAAGAACTTCTGTGGCTTCGCTGCCACCATATTTTCTGTCATCATGTATGGTTCTCCTCTCTCAATTATGAGATTAGTAATCAAAAGCAAAAGTGTTGAGTTCATGCCATTCTTCTTGTCACTGTTTGTGTTTCTTTGTGGCACTTCTTGGTTTATCTTTGGTCTACTAGGTCATGACCTATTTATTGCGGTACCTAATGGACTTGGTTCTGTTTTGGGGACAATACAACTTATATTGTATTTCATATACCGTGACAATAAAGGTATTCCAAAAAAACATGGTACAACAGAGGAGAGTTCAATGGAAATGGGCAATGGAAAATCACATCAAATGAAACAGTCCAATGCAAATGAAATTCAAGGGTGACATTCAAGGACATTGTGTCATTCAAAACAATATATGTGTTTTTCAGCTACATAATATGTAACTGCTCCTATTTTTGTTTCTTAAATGACAATTATTTGCTTGTGTTAAGAGTCTGGCACCTTTAGGAGTTTCTTTGGTACTTCCCATTGTTGAGACATATATACATGGGTGAGTGAGTGAGTTTCTCTTAAATGATGTTCTTTTTCCTTGTAGCCTAAAAAAATAAAACTTGTAACTAATTGTTNTRAGTT
TARGET=1193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04787 Ps_contig_mira-and-tgicl-ass_franssen_34643
SEQUENCE=GGTTTTAACCTAATGAATTGGTTAGTAAGAGAAGTAATAACATAGATGACAGTATATTTTTCAATTTCAATCCTAATTGAATTGCATTATTGATACACTCAATGAATAAACTCTTTCAAAACATAACAGTGAAGATACAAAAAGAAAAAGGAACCGTTGTAAGTGAACTTTGATTCATCAGAGACACAGAAAAAATGTTCGTACAGTTTGTTTCAGAACAGTCGATTAGAAGCAGCCGCCCAACAAAACTGCCTCAATCTCCTCATTGGTGACCGGAGTTCTTTTGGGCTGAAGAGAGGCTTTTCCTCCTAATGTTGTCGAAGCATTTGAACTTGAAAAGAACGATAAACCGCCATCGCCTGTCGAAGACAAAGCTTGACCCTCAGAGGCAGAAGCAGAGGATTTTAGGTGCCTGTTGGGGAACTTGATGCGAGGGATTCTCTTCAAAGCATGAGCACCACCCATCGTTGAAACAAAACAAATAACCTTCGAGAAATCAACACAACACAACACTTCTCCGAAATGTCTTCTTAAGGCAGCCCCNCCAAACCAAACCTAAACCTACCTTTAACC
TARGET=498,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2626
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=0.249232
PRIMER_LEFT_SEQUENCE=GAGGCAGAAGCAGAGGATTTTA
PRIMER_RIGHT_SEQUENCE=CTGCCTTAAGAAGACATTTCGG
PRIMER_LEFT=385,22
PRIMER_RIGHT=538,22
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=60.249
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.3234
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=0.310002
PRIMER_LEFT_1_SEQUENCE=GAGGCAGAAGCAGAGGATTTTA
PRIMER_RIGHT_1_SEQUENCE=TAGGTTTAGGTTTGGTTTGGNG
PRIMER_LEFT_1=385,22
PRIMER_RIGHT_1=563,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=59.690
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.8250
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=179
PRIMER_PAIR_PENALTY_2=0.3772
PRIMER_LEFT_2_PENALTY=0.127927
PRIMER_RIGHT_2_PENALTY=0.249232
PRIMER_LEFT_2_SEQUENCE=CTTGAAAAGAACGATAAACCGC
PRIMER_RIGHT_2_SEQUENCE=CTGCCTTAAGAAGACATTTCGG
PRIMER_LEFT_2=331,22
PRIMER_RIGHT_2=538,22
PRIMER_LEFT_2_TM=60.128
PRIMER_RIGHT_2_TM=60.249
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.3783
PRIMER_LEFT_3_PENALTY=0.129103
PRIMER_RIGHT_3_PENALTY=0.249232
PRIMER_LEFT_3_SEQUENCE=GCTTTTCCTCCTAATGTTGTCG
PRIMER_RIGHT_3_SEQUENCE=CTGCCTTAAGAAGACATTTCGG
PRIMER_LEFT_3=298,22
PRIMER_RIGHT_3=538,22
PRIMER_LEFT_3_TM=60.129
PRIMER_RIGHT_3_TM=60.249
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=0.3964
PRIMER_LEFT_4_PENALTY=0.013401
PRIMER_RIGHT_4_PENALTY=0.382951
PRIMER_LEFT_4_SEQUENCE=GAGGCAGAAGCAGAGGATTTTA
PRIMER_RIGHT_4_SEQUENCE=GCTGCCTTAAGAAGACATTTCG
PRIMER_LEFT_4=385,22
PRIMER_RIGHT_4=539,22
PRIMER_LEFT_4_TM=59.987
PRIMER_RIGHT_4_TM=60.383
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_04788 Ps_contig_mira-and-tgicl-ass_franssen_34644
SEQUENCE=GANCCNTTTTTTTTAGGAGAGAGAGAGAGATGGAGTTATCAAAGTTTAAGAGAATTTGTGTGTTTTGTGGTAGTAGTCCTGGTAATAAAACTAGCTATAAAGATGCTGCTATTGAACTTGGAAGAGAATTGGTTTCAAGAAATATTGACTTAGTTTATGGAGGAGGCAGCATTGGTTTGATGGGCTTGATTTCTCAAGCCGTTTACGACGGCGGTCGACACGTAATCGGAGTGATTCCCAAAACACTTATGCCAAGAGAGCTTACTGGAGAAACAGTGGGAGAAGTGAAGGCAGTGGCTGACATGCATCAAAGGAAAGCTGAAATGGCTAGAAATTCTGATGCTTTTATTGCTTTACCTGGTGGCTATGGTACACTTGAGGAGCTTCTTGAGGTGATAACATGGGCACAACTTGGCATCCATGATAAACCAGTGGGTTTGCTAAATGTTGATGGATACTACAATTCTTTTTTGTCTTTCATTGACAAAGCTGTGGAGGAAGGGTTCATTAGCCCCACAGCTCGTCATATAATTGTATCTGCCCCAACACCAAAAGAACTTGTCAAAAACATGGAGGAATATTTCCCACAGCACGAAAGAGTTGCCTCAAAGCTAACATGGGAAAGTGATCAGCTAGATTAGATGGTGTAGAGAACAATTGTTAGTGAGAATGTGAGACAATTCAAGGGATTGATATAGTAGAAGATCATAATTTCATGGAGCCAGGATTTTTTGTGTGCTCTTAGATTTCCTTGTTAGTGTAAAACTAAAGATTTGGAATCTAGCTAGCTATCAATGCTAACACTACTATATGCTCAATATGAATTCCGTTTTGTTTTTAAGAACGTANTTTGTTGACTTAAAATAAAAAATTAGAGCACTTAATTTTAAAGGGTTACCCGAAAAAAAAACC
TARGET=17,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04789 Ps_contig_mira-and-tgicl-ass_franssen_34650
SEQUENCE=TTCGGGGNACCCCTGTTTCAAAACACTCTAAACAATTCAACCTCGGTCTCTGCTCTATCTATCTCCTTGTTCAATTCAATTTTCACAATTCCAAACAAAAAAGACCCCTCAAATCTCAACAAACTCAACTAACCATCGATCAACTCTGTGCAGAAAACTACCACCACCACCAACAACAACAACAACAGCTTCACCTCTTCATCAATGGAGTTTTTCCAACGCGCCAAAGCCGTTCGCCTCCGAAGCCACCACGACAAATACCTCCACGCCGAAGAAGACGAAGAATCCATCAACCAAGAACGCAACGGCTCATCCAAAAACGCCAAATGGACAGTCGAACACATCCCCGAATTCGACAACATCATCCGCCTCAAAAGCTGTTACGGCAAATACTTAACAGCCTCCAATCAGCCGTTACTCCTTGGCGTCACCGGCCGCAAAGTCGTTCAAACACTGCCGCGGAGACTCGACTCCTCCGTTGAATGGGAGCCTGTTAGAGACGGTGCGCAGGTTAAGCTGAAAACGAGATACGGTAATTTTCTGAGAGCTAATGGTGGGTTACCACCTTGGAGAAATTCTGTTACGCATGATATTCCTCACAGGAGTGCTACGCAGGATTGGATTCTTTGGGATGTTGATGTTCTTGAGATTCATGTTGGGAATCCTCCTCCTCCTCCGATTCCTCATTCGGATTCTCTTGACTTTGGATCTTCGGCGCTTTCTTTCAAATCTAATCGGTTCGATAGACAAGAGTCAACAGATAGTGTTGGTTCGCCACCGAAGATGGAGGGGAGAACGATATACTACCATGTTGCTGAGGAAAATGGAGACGTGGATGATGAGAATGTGCAGGGCTATTCCTTAGTCTTCAATGGGAATGATGTCGAGCAGTTGACTCGTAAGTTCGTGGAAGAAACCGGACTTGATGGAGTTATTGTGTGCAGTCGAAGTCCTTTGAATGGAAAACTTTACCCTCTTCGCTTGCATCTTCCTCCAAACAATGTTACAATGCAAGTTGTTTTGGTCCTTGCCTCCTCGAAAGTTGCAAAAGAATTTGAAGAACAGGGTTTACTGTGACCAGCTATATCTTTGGAGTCTTAAACCTATTAAAGTGCAAGGCCAATTTACATTGTAACCCATTGGGTTTCAATTCTATTTTATAGCATTTAATTTAAAAATATGCTGAAAGACTCCACCTTGCTCGAGCGAGACTACAAGACTGTAGGCGATCTCGCCGTTGTTAATGCTAGCTTTGGATTTGGGTACTTTGTTGTTGTAATTTACTGTTATTCATTATTATTTTTTTAGTATGTATCTCTTGTATAGAGATCATTAATCAGAAAGGTGCAGTCATGGTATTTTTTTCAGGCCGATATAAATGTCAATTAGTTTCTGTACTTTAAAAAAT
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0769
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.053348
PRIMER_LEFT_SEQUENCE=CCCCTCAAATCTCAACAAACTC
PRIMER_RIGHT_SEQUENCE=GGTTGATGGATTCTTCGTCTTC
PRIMER_LEFT=104,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.947
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.0769
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.053348
PRIMER_LEFT_1_SEQUENCE=AGACCCCTCAAATCTCAACAAA
PRIMER_RIGHT_1_SEQUENCE=GGTTGATGGATTCTTCGTCTTC
PRIMER_LEFT_1=101,22
PRIMER_RIGHT_1=294,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.947
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=194
PRIMER_PAIR_PENALTY_2=0.0769
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.053348
PRIMER_LEFT_2_SEQUENCE=AAAGACCCCTCAAATCTCAACA
PRIMER_RIGHT_2_SEQUENCE=GGTTGATGGATTCTTCGTCTTC
PRIMER_LEFT_2=99,22
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=59.947
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.0769
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.053348
PRIMER_LEFT_3_SEQUENCE=AAGACCCCTCAAATCTCAACAA
PRIMER_RIGHT_3_SEQUENCE=GGTTGATGGATTCTTCGTCTTC
PRIMER_LEFT_3=100,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=59.947
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=0.1482
PRIMER_LEFT_4_PENALTY=0.023556
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=CCCCTCAAATCTCAACAAACTC
PRIMER_RIGHT_4_SEQUENCE=CATTGATGAAGAGGTGAAGCTG
PRIMER_LEFT_4=104,22
PRIMER_RIGHT_4=206,22
PRIMER_LEFT_4_TM=59.976
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_04790 Ps_contig_mira-and-tgicl-ass_franssen_34650
SEQUENCE=TTCGGGGNACCCCTGTTTCAAAACACTCTAAACAATTCAACCTCGGTCTCTGCTCTATCTATCTCCTTGTTCAATTCAATTTTCACAATTCCAAACAAAAAAGACCCCTCAAATCTCAACAAACTCAACTAACCATCGATCAACTCTGTGCAGAAAACTACCACCACCACCAACAACAACAACAACAGCTTCACCTCTTCATCAATGGAGTTTTTCCAACGCGCCAAAGCCGTTCGCCTCCGAAGCCACCACGACAAATACCTCCACGCCGAAGAAGACGAAGAATCCATCAACCAAGAACGCAACGGCTCATCCAAAAACGCCAAATGGACAGTCGAACACATCCCCGAATTCGACAACATCATCCGCCTCAAAAGCTGTTACGGCAAATACTTAACAGCCTCCAATCAGCCGTTACTCCTTGGCGTCACCGGCCGCAAAGTCGTTCAAACACTGCCGCGGAGACTCGACTCCTCCGTTGAATGGGAGCCTGTTAGAGACGGTGCGCAGGTTAAGCTGAAAACGAGATACGGTAATTTTCTGAGAGCTAATGGTGGGTTACCACCTTGGAGAAATTCTGTTACGCATGATATTCCTCACAGGAGTGCTACGCAGGATTGGATTCTTTGGGATGTTGATGTTCTTGAGATTCATGTTGGGAATCCTCCTCCTCCTCCGATTCCTCATTCGGATTCTCTTGACTTTGGATCTTCGGCGCTTTCTTTCAAATCTAATCGGTTCGATAGACAAGAGTCAACAGATAGTGTTGGTTCGCCACCGAAGATGGAGGGGAGAACGATATACTACCATGTTGCTGAGGAAAATGGAGACGTGGATGATGAGAATGTGCAGGGCTATTCCTTAGTCTTCAATGGGAATGATGTCGAGCAGTTGACTCGTAAGTTCGTGGAAGAAACCGGACTTGATGGAGTTATTGTGTGCAGTCGAAGTCCTTTGAATGGAAAACTTTACCCTCTTCGCTTGCATCTTCCTCCAAACAATGTTACAATGCAAGTTGTTTTGGTCCTTGCCTCCTCGAAAGTTGCAAAAGAATTTGAAGAACAGGGTTTACTGTGACCAGCTATATCTTTGGAGTCTTAAACCTATTAAAGTGCAAGGCCAATTTACATTGTAACCCATTGGGTTTCAATTCTATTTTATAGCATTTAATTTAAAAATATGCTGAAAGACTCCACCTTGCTCGAGCGAGACTACAAGACTGTAGGCGATCTCGCCGTTGTTAATGCTAGCTTTGGATTTGGGTACTTTGTTGTTGTAATTTACTGTTATTCATTATTATTTTTTTAGTATGTATCTCTTGTATAGAGATCATTAATCAGAAAGGTGCAGTCATGGTATTTTTTTCAGGCCGATATAAATGTCAATTAGTTTCTGTACTTTAAAAAAT
TARGET=172,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0769
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.053348
PRIMER_LEFT_SEQUENCE=CCCCTCAAATCTCAACAAACTC
PRIMER_RIGHT_SEQUENCE=GGTTGATGGATTCTTCGTCTTC
PRIMER_LEFT=104,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.947
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.0769
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.053348
PRIMER_LEFT_1_SEQUENCE=AGACCCCTCAAATCTCAACAAA
PRIMER_RIGHT_1_SEQUENCE=GGTTGATGGATTCTTCGTCTTC
PRIMER_LEFT_1=101,22
PRIMER_RIGHT_1=294,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.947
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=194
PRIMER_PAIR_PENALTY_2=0.0769
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.053348
PRIMER_LEFT_2_SEQUENCE=AAAGACCCCTCAAATCTCAACA
PRIMER_RIGHT_2_SEQUENCE=GGTTGATGGATTCTTCGTCTTC
PRIMER_LEFT_2=99,22
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=59.947
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.0769
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.053348
PRIMER_LEFT_3_SEQUENCE=AAGACCCCTCAAATCTCAACAA
PRIMER_RIGHT_3_SEQUENCE=GGTTGATGGATTCTTCGTCTTC
PRIMER_LEFT_3=100,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=59.947
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=0.1931
PRIMER_LEFT_4_PENALTY=0.139753
PRIMER_RIGHT_4_PENALTY=0.053348
PRIMER_LEFT_4_SEQUENCE=TAACCATCGATCAACTCTGTGC
PRIMER_RIGHT_4_SEQUENCE=GGTTGATGGATTCTTCGTCTTC
PRIMER_LEFT_4=129,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=60.140
PRIMER_RIGHT_4_TM=59.947
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_04791 Ps_contig_mira-and-tgicl-ass_franssen_34650
SEQUENCE=TTCGGGGNACCCCTGTTTCAAAACACTCTAAACAATTCAACCTCGGTCTCTGCTCTATCTATCTCCTTGTTCAATTCAATTTTCACAATTCCAAACAAAAAAGACCCCTCAAATCTCAACAAACTCAACTAACCATCGATCAACTCTGTGCAGAAAACTACCACCACCACCAACAACAACAACAACAGCTTCACCTCTTCATCAATGGAGTTTTTCCAACGCGCCAAAGCCGTTCGCCTCCGAAGCCACCACGACAAATACCTCCACGCCGAAGAAGACGAAGAATCCATCAACCAAGAACGCAACGGCTCATCCAAAAACGCCAAATGGACAGTCGAACACATCCCCGAATTCGACAACATCATCCGCCTCAAAAGCTGTTACGGCAAATACTTAACAGCCTCCAATCAGCCGTTACTCCTTGGCGTCACCGGCCGCAAAGTCGTTCAAACACTGCCGCGGAGACTCGACTCCTCCGTTGAATGGGAGCCTGTTAGAGACGGTGCGCAGGTTAAGCTGAAAACGAGATACGGTAATTTTCTGAGAGCTAATGGTGGGTTACCACCTTGGAGAAATTCTGTTACGCATGATATTCCTCACAGGAGTGCTACGCAGGATTGGATTCTTTGGGATGTTGATGTTCTTGAGATTCATGTTGGGAATCCTCCTCCTCCTCCGATTCCTCATTCGGATTCTCTTGACTTTGGATCTTCGGCGCTTTCTTTCAAATCTAATCGGTTCGATAGACAAGAGTCAACAGATAGTGTTGGTTCGCCACCGAAGATGGAGGGGAGAACGATATACTACCATGTTGCTGAGGAAAATGGAGACGTGGATGATGAGAATGTGCAGGGCTATTCCTTAGTCTTCAATGGGAATGATGTCGAGCAGTTGACTCGTAAGTTCGTGGAAGAAACCGGACTTGATGGAGTTATTGTGTGCAGTCGAAGTCCTTTGAATGGAAAACTTTACCCTCTTCGCTTGCATCTTCCTCCAAACAATGTTACAATGCAAGTTGTTTTGGTCCTTGCCTCCTCGAAAGTTGCAAAAGAATTTGAAGAACAGGGTTTACTGTGACCAGCTATATCTTTGGAGTCTTAAACCTATTAAAGTGCAAGGCCAATTTACATTGTAACCCATTGGGTTTCAATTCTATTTTATAGCATTTAATTTAAAAATATGCTGAAAGACTCCACCTTGCTCGAGCGAGACTACAAGACTGTAGGCGATCTCGCCGTTGTTAATGCTAGCTTTGGATTTGGGTACTTTGTTGTTGTAATTTACTGTTATTCATTATTATTTTTTTAGTATGTATCTCTTGTATAGAGATCATTAATCAGAAAGGTGCAGTCATGGTATTTTTTTCAGGCCGATATAAATGTCAATTAGTTTCTGTACTTTAAAAAAT
TARGET=663,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0218
PRIMER_LEFT_PENALTY=0.018437
PRIMER_RIGHT_PENALTY=0.003322
PRIMER_LEFT_SEQUENCE=TACGCATGATATTCCTCACAGG
PRIMER_RIGHT_SEQUENCE=CACATTCTCATCATCCACGTCT
PRIMER_LEFT=581,22
PRIMER_RIGHT=848,22
PRIMER_LEFT_TM=59.982
PRIMER_RIGHT_TM=59.997
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=0.0326
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.003322
PRIMER_LEFT_1_SEQUENCE=TGTTCTTGAGATTCATGTTGGG
PRIMER_RIGHT_1_SEQUENCE=CACATTCTCATCATCCACGTCT
PRIMER_LEFT_1=638,22
PRIMER_RIGHT_1=848,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=59.997
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.0326
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.003322
PRIMER_LEFT_2_SEQUENCE=TTGGGATGTTGATGTTCTTGAG
PRIMER_RIGHT_2_SEQUENCE=CACATTCTCATCATCCACGTCT
PRIMER_LEFT_2=626,22
PRIMER_RIGHT_2=848,22
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=59.997
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.0492
PRIMER_LEFT_3_PENALTY=0.029253
PRIMER_RIGHT_3_PENALTY=0.019966
PRIMER_LEFT_3_SEQUENCE=TGTTCTTGAGATTCATGTTGGG
PRIMER_RIGHT_3_SEQUENCE=CACGAACTTACGAGTCAACTGC
PRIMER_LEFT_3=638,22
PRIMER_RIGHT_3=908,22
PRIMER_LEFT_3_TM=59.971
PRIMER_RIGHT_3_TM=59.980
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=271
PRIMER_PAIR_PENALTY_4=0.0492
PRIMER_LEFT_4_PENALTY=0.029253
PRIMER_RIGHT_4_PENALTY=0.019966
PRIMER_LEFT_4_SEQUENCE=TTGGGATGTTGATGTTCTTGAG
PRIMER_RIGHT_4_SEQUENCE=CACGAACTTACGAGTCAACTGC
PRIMER_LEFT_4=626,22
PRIMER_RIGHT_4=908,22
PRIMER_LEFT_4_TM=59.971
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_04792 Ps_contig_mira-and-tgicl-ass_franssen_34651
SEQUENCE=CGCCTTGGATCATCGGCGCCAAAGTTTGAGCAACACTTAATCTCGTCACCGCGTTTGAATCACTCTACTACGTCATTTGGACAATACTCGGTTCCGTCTTCTTCATCGCCGTTCTTCAATATGAGTGATATTCCAAATCAATCCTTTGAAGAACACCAAGGACAATTCTCAAACAAGCACAACAACAACAACTACATGGTCTAATGCAACTTCCTGATCTTCAAGGAAACACGACAAGTAATAACTCGAATAATATTTTCAACCTCATGAATAATATTCATGAAGATCAATTCAACAACAATCCTCTTATTAGTGATCATCATCATCAATCACTCTTTATGGTCAACTCAAATATGCAACAACATGATCATCAAAACCAAAACCAAAACCAAAACCTGTCTAGTCCTCACATGTCAGCCACTGCATTACTTCAGAAAGCTTCTCAAATTGGTTCAACTAATTCAACCAACAACAACAAAGGTACTACAAATACTACTACTACTACCACATCTTTTGGAAATAGAAGTAGTTCAAGCATGGAACTAAATGATCATAACAACAATGATGAACTTCATGGATTGATAAACTCTATTGCAAATGGAAATGGAAACACATCCTCCATATTTGGCAATGAAAGTAACCTTAATATAGGGTTTGGTGAATCTGATAAGTTAACATTAGATTTTCTTGGTGTTGGTGGAATGGTCAGAAACATGAGTGCTCCTGGATTTTCACAAAATGAACAACAACATCATCAACAACAACAACATGTCATGGCTTCTATGAATCATAACCTAAAATCAGCTYAGTCAAGTCAACATTTCGGAAGCTCAAATTTGATGCAATGAAAGAAAAATAAAGGAAATATTGCTTTCTACT
TARGET=179,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04793 Ps_contig_mira-and-tgicl-ass_franssen_34651
SEQUENCE=CGCCTTGGATCATCGGCGCCAAAGTTTGAGCAACACTTAATCTCGTCACCGCGTTTGAATCACTCTACTACGTCATTTGGACAATACTCGGTTCCGTCTTCTTCATCGCCGTTCTTCAATATGAGTGATATTCCAAATCAATCCTTTGAAGAACACCAAGGACAATTCTCAAACAAGCACAACAACAACAACTACATGGTCTAATGCAACTTCCTGATCTTCAAGGAAACACGACAAGTAATAACTCGAATAATATTTTCAACCTCATGAATAATATTCATGAAGATCAATTCAACAACAATCCTCTTATTAGTGATCATCATCATCAATCACTCTTTATGGTCAACTCAAATATGCAACAACATGATCATCAAAACCAAAACCAAAACCAAAACCTGTCTAGTCCTCACATGTCAGCCACTGCATTACTTCAGAAAGCTTCTCAAATTGGTTCAACTAATTCAACCAACAACAACAAAGGTACTACAAATACTACTACTACTACCACATCTTTTGGAAATAGAAGTAGTTCAAGCATGGAACTAAATGATCATAACAACAATGATGAACTTCATGGATTGATAAACTCTATTGCAAATGGAAATGGAAACACATCCTCCATATTTGGCAATGAAAGTAACCTTAATATAGGGTTTGGTGAATCTGATAAGTTAACATTAGATTTTCTTGGTGTTGGTGGAATGGTCAGAAACATGAGTGCTCCTGGATTTTCACAAAATGAACAACAACATCATCAACAACAACAACATGTCATGGCTTCTATGAATCATAACCTAAAATCAGCTYAGTCAAGTCAACATTTCGGAAGCTCAAATTTGATGCAATGAAAGAAAAATAAAGGAAATATTGCTTTCTACT
TARGET=316,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04794 Ps_contig_mira-and-tgicl-ass_franssen_34651
SEQUENCE=CGCCTTGGATCATCGGCGCCAAAGTTTGAGCAACACTTAATCTCGTCACCGCGTTTGAATCACTCTACTACGTCATTTGGACAATACTCGGTTCCGTCTTCTTCATCGCCGTTCTTCAATATGAGTGATATTCCAAATCAATCCTTTGAAGAACACCAAGGACAATTCTCAAACAAGCACAACAACAACAACTACATGGTCTAATGCAACTTCCTGATCTTCAAGGAAACACGACAAGTAATAACTCGAATAATATTTTCAACCTCATGAATAATATTCATGAAGATCAATTCAACAACAATCCTCTTATTAGTGATCATCATCATCAATCACTCTTTATGGTCAACTCAAATATGCAACAACATGATCATCAAAACCAAAACCAAAACCAAAACCTGTCTAGTCCTCACATGTCAGCCACTGCATTACTTCAGAAAGCTTCTCAAATTGGTTCAACTAATTCAACCAACAACAACAAAGGTACTACAAATACTACTACTACTACCACATCTTTTGGAAATAGAAGTAGTTCAAGCATGGAACTAAATGATCATAACAACAATGATGAACTTCATGGATTGATAAACTCTATTGCAAATGGAAATGGAAACACATCCTCCATATTTGGCAATGAAAGTAACCTTAATATAGGGTTTGGTGAATCTGATAAGTTAACATTAGATTTTCTTGGTGTTGGTGGAATGGTCAGAAACATGAGTGCTCCTGGATTTTCACAAAATGAACAACAACATCATCAACAACAACAACATGTCATGGCTTCTATGAATCATAACCTAAAATCAGCTYAGTCAAGTCAACATTTCGGAAGCTCAAATTTGATGCAATGAAAGAAAAATAAAGGAAATATTGCTTTCTACT
TARGET=467,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04795 Ps_contig_mira-and-tgicl-ass_franssen_34651
SEQUENCE=CGCCTTGGATCATCGGCGCCAAAGTTTGAGCAACACTTAATCTCGTCACCGCGTTTGAATCACTCTACTACGTCATTTGGACAATACTCGGTTCCGTCTTCTTCATCGCCGTTCTTCAATATGAGTGATATTCCAAATCAATCCTTTGAAGAACACCAAGGACAATTCTCAAACAAGCACAACAACAACAACTACATGGTCTAATGCAACTTCCTGATCTTCAAGGAAACACGACAAGTAATAACTCGAATAATATTTTCAACCTCATGAATAATATTCATGAAGATCAATTCAACAACAATCCTCTTATTAGTGATCATCATCATCAATCACTCTTTATGGTCAACTCAAATATGCAACAACATGATCATCAAAACCAAAACCAAAACCAAAACCTGTCTAGTCCTCACATGTCAGCCACTGCATTACTTCAGAAAGCTTCTCAAATTGGTTCAACTAATTCAACCAACAACAACAAAGGTACTACAAATACTACTACTACTACCACATCTTTTGGAAATAGAAGTAGTTCAAGCATGGAACTAAATGATCATAACAACAATGATGAACTTCATGGATTGATAAACTCTATTGCAAATGGAAATGGAAACACATCCTCCATATTTGGCAATGAAAGTAACCTTAATATAGGGTTTGGTGAATCTGATAAGTTAACATTAGATTTTCTTGGTGTTGGTGGAATGGTCAGAAACATGAGTGCTCCTGGATTTTCACAAAATGAACAACAACATCATCAACAACAACAACATGTCATGGCTTCTATGAATCATAACCTAAAATCAGCTYAGTCAAGTCAACATTTCGGAAGCTCAAATTTGATGCAATGAAAGAAAAATAAAGGAAATATTGCTTTCTACT
TARGET=491,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04796 Ps_contig_mira-and-tgicl-ass_franssen_34651
SEQUENCE=CGCCTTGGATCATCGGCGCCAAAGTTTGAGCAACACTTAATCTCGTCACCGCGTTTGAATCACTCTACTACGTCATTTGGACAATACTCGGTTCCGTCTTCTTCATCGCCGTTCTTCAATATGAGTGATATTCCAAATCAATCCTTTGAAGAACACCAAGGACAATTCTCAAACAAGCACAACAACAACAACTACATGGTCTAATGCAACTTCCTGATCTTCAAGGAAACACGACAAGTAATAACTCGAATAATATTTTCAACCTCATGAATAATATTCATGAAGATCAATTCAACAACAATCCTCTTATTAGTGATCATCATCATCAATCACTCTTTATGGTCAACTCAAATATGCAACAACATGATCATCAAAACCAAAACCAAAACCAAAACCTGTCTAGTCCTCACATGTCAGCCACTGCATTACTTCAGAAAGCTTCTCAAATTGGTTCAACTAATTCAACCAACAACAACAAAGGTACTACAAATACTACTACTACTACCACATCTTTTGGAAATAGAAGTAGTTCAAGCATGGAACTAAATGATCATAACAACAATGATGAACTTCATGGATTGATAAACTCTATTGCAAATGGAAATGGAAACACATCCTCCATATTTGGCAATGAAAGTAACCTTAATATAGGGTTTGGTGAATCTGATAAGTTAACATTAGATTTTCTTGGTGTTGGTGGAATGGTCAGAAACATGAGTGCTCCTGGATTTTCACAAAATGAACAACAACATCATCAACAACAACAACATGTCATGGCTTCTATGAATCATAACCTAAAATCAGCTYAGTCAAGTCAACATTTCGGAAGCTCAAATTTGATGCAATGAAAGAAAAATAAAGGAAATATTGCTTTCTACT
TARGET=756,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04797 Ps_contig_mira-and-tgicl-ass_franssen_34653
SEQUENCE=AACTAAGAAAATCTATTTTTNATAACTAATTTAAGATATTTTGTGGCAGTTATCTTATCCTCCCCAAAACACTCTGCAATGTTTTGTTCGTTATGCAACCTTTCTATAACCAAACCCATTGTTGTTACCAATGCCACCCTTCCTCAAAACCCAGATTCTTCTTCTTCTTCTTCTTCATCTTCACCTCAACAAACCAATCTTCCCCGAAGTAAAAAACCTTCACTAGGCCCCACAAACTCCACTACTTCTTCACCACCAAACAAGCTTCCCCGTCGACCTCCACAGCCTTCAATTATGCAAATTGAACGTGCCCTCGGCGCCGGAAGCTTCCGAGACGGCGAGCCTGAGCTCAAAAAGGAGAAAGATTCAGATGCGAAGAAGACTACAATGGATTTGTTCTTGGGGCAAGCATTTGAAGGGGTGATGCAGAAAAAGCTGAGGGAAACAGGAGAGTGGCTTGGTGAAAATGGAGAGACCAGATTCCGCTCCACTAAGACTAGAAAAGGAATTCTGGTGTTTGCGTTTCAATGGATGATGCCAATTTGGGTCTTATTATTTCTAATAGCATCTGGAGCCATCAAGTTACCATTTAGTACCCCATTTCTTGAGGACCTCCTCATGTGAACATTGGCCAGTTGCTTTTGTAGATTGAATAACTATTAATTACTCAAAAGTTCAAAGGAACACTGCATACATTTGTATTTAATAGTTTAACATTTTGGAAGTTCAGTCTTTTGGGAGTTGTACAGTCACTTCACTTATTTGATTGAACTTGTATTAATTAAATGTCAAATATTGTAAAATATTTATCCATGTAGCTCACA
TARGET=156,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2513
PRIMER_LEFT_PENALTY=0.107763
PRIMER_RIGHT_PENALTY=0.143555
PRIMER_LEFT_SEQUENCE=AACCAAACCCATTGTTGTTACC
PRIMER_RIGHT_SEQUENCE=CGCATCTGAATCTTTCTCCTTT
PRIMER_LEFT=107,22
PRIMER_RIGHT=374,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=59.856
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=0.4478
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.340007
PRIMER_LEFT_1_SEQUENCE=AACCAAACCCATTGTTGTTACC
PRIMER_RIGHT_1_SEQUENCE=TCGCATCTGAATCTTTCTCCTT
PRIMER_LEFT_1=107,22
PRIMER_RIGHT_1=375,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.340
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=0.4478
PRIMER_LEFT_2_PENALTY=0.107763
PRIMER_RIGHT_2_PENALTY=0.340007
PRIMER_LEFT_2_SEQUENCE=AACCAAACCCATTGTTGTTACC
PRIMER_RIGHT_2_SEQUENCE=TTCGCATCTGAATCTTTCTCCT
PRIMER_LEFT_2=107,22
PRIMER_RIGHT_2=376,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=60.340
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=270
PRIMER_PAIR_PENALTY_3=0.4478
PRIMER_LEFT_3_PENALTY=0.107763
PRIMER_RIGHT_3_PENALTY=0.340007
PRIMER_LEFT_3_SEQUENCE=AACCAAACCCATTGTTGTTACC
PRIMER_RIGHT_3_SEQUENCE=CTTCGCATCTGAATCTTTCTCC
PRIMER_LEFT_3=107,22
PRIMER_RIGHT_3=377,22
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=60.340
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=271
PRIMER_PAIR_PENALTY_4=0.4746
PRIMER_LEFT_4_PENALTY=0.107763
PRIMER_RIGHT_4_PENALTY=0.366796
PRIMER_LEFT_4_SEQUENCE=AACCAAACCCATTGTTGTTACC
PRIMER_RIGHT_4_SEQUENCE=TTGGTGGTGAAGAAGTAGTGGA
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=258,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=59.633
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_04798 Ps_contig_mira-and-tgicl-ass_franssen_34654
SEQUENCE=ACATGCTGTTAAGGTAAGTAACTACTCTCGTTGTCGTGGTGGTCTTCAGAGAGNTTACTTNTAAGCGTTTGGAGAAGGAAGGAAGGAGTAAGCTTTTGTACGCGAACAACGCATGATGATAATGCTATAACAAATGCAGTTGAAATATATGAAAGCCTGAGTGGTGCAACTCATTTTATAGCTGCTAATAATGATTCTGGCGTTAGAGAATATGACATAGAAAAGTTTCAGCTTTTGAATCATGTGCAATTCCCTTGGCCGGTGAATCACACATCGATCAGTCCTGACCGTAAACTAATGACTGTTGTGGGGGACAACTTAGATGGACTGCTGGTGGATCCTCAAAATGGGAAGACTGTCGCAACTTTGACTGGTCATCAAGATTACTCCTTTGCATCCGCATGGCATCCTAACGGCCACGCATTCGCAACCGGGAATCAAGACAAGACATGCAGAGTATGGGACGCTAGAAATGTGTCATCTCCACTCGCCATTCTCAAGAACAACCTTGGTGCGAGCCGGTCGATTCGGTTTTCTTCAGACGGACAGTACATGGCTGTTGCTGAACCTGCTGATTTCGTGCATGTCTATAATACAAAGTCGAATTACGAAAAAAGTCAAGAGATCGATTTCTTTGGCGAAATTTCCGGAGTTTCGGTAAGTCCGGATGATGAGTGTATGTATATTGGAATATGGGACAGGACTTATGCTAGCTTACTACAGTATAATAAGAGACACTCATATCATTATCTGGATTCTTGCTATTGATCTTGGTTACTTTTTTTCTTACTTTTGGATTGATGTTAATTTTATAGAGCATTGTGGAATTATAGATATACTTTACATTTTGGTTTTGGTAGTATTTTTTTGTGTAAGTCATATAAATCAGGCCTCATGTTGTATTTCTAAACTTTTGATGTAAATGCCACCAGAAAAAAATTGGGAATTGCTTARGAA
TARGET=74,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04799 Ps_contig_mira-and-tgicl-ass_franssen_34655
SEQUENCE=ATCCTCCATATCTTTTCTTTCTAAAGGAAAATCAAATTTGGGTGTGGACGAAAAAAACAAATTTTAAGAAACTTTCAATCCTCACAGTATCTCTTTCCTTCCACTTTTATCTATACCAAACAAACCATAAGCGTTGCCCCTAGAAAGAAGAAATCTAAAAAGAACCTTCCAGACTCTGATATAAGAACGAAATCTCTCGCTTTCCTCTCAATTTCGATTAACCTACTTCTGATATCAGGTTCAGGAATTAAATTGAAATCTTAAGATATGCCGGCTACAGCAGGTAGGGTTCGCATGCCTGCGAACAATAGGGTTCACAGTAGTGCAGCCCTTCAAACACATGGCATATGGCAAAGTGCTATTGGCTATGACCCTTATGCACCCAATAAGGAAGACAAGGACACTTCTCAAAGTCAGCCAAGTGCAGAACCTGATGCAGAGAATGCATATGCCAGCTTCCAAGGGCTTCTACAGCTTGCTAAGATAACCAATGCCGACGTGGATGTGTCCCGTGGAGCCTGCAAGAGGTGTGGCCGTGTTGGGCACCTCAAGTTTCAGTGCAAGAACTACGTGAAAATCAAAGATGACGAGAAGGATCCTGAAGCATTGCAGTCTCTGGTTGGATTGGATAAGTTGAAGGGTAAGTCTGAGAGGGTGGATAGGAAAAGCAATGTAGAGAGCTCAGAAGAGGAGGAAGAGGATAGCGAGAGTTCAGATTCTGAGTTTGATTCAGAGATTGAGAGGGCTATTGCTGAAAGGTCAGGGAAGAAAGTTAGTGGGAAGAGAGGTTCTTATAGAAAGGAGGACTCAGATGATGATGAGTCAGACAAAGATTCTGACCGGAAGAGGAGAAAGAGAGGCAGAGCAAAGAAGAGGAGTGTTAAGACTAATAGGGCAGCTAGTGATTCGGATGATTCCGGTGAAAGGAGGAGGAGGAAGAAAAAGAGGGAGCACAGAAGAAGCAGAAGGGACAAGTCTCCAGATGATAGTGATGAACATAGTCACAAAAAAAGGAAGAGTAGGAAGGAGAAGAGGAGAAGGAGAAGCCGTCTGTCAGATTCTGATTCTGAAACATCTGAGGATTCCTCTTCCCGTCACAAGCGGAAAAGCAAGAAGACACTGTCGTCATCCGACAATGATTCTAGTGATGGATATAATGATTCAAGGAAGGGTAGGGAAGTTAAAAAATCAGAAAAGAGGAGGAGACGCCACCATGGAGATGACGATTAGAGGGGCAATGGAGTAGTCAATCTCATGCGTCTAAAGTTTTATGTATCATAAACCTATATATGCCAATATTGCACTTGCTTATGGTTGAGCTTCAAAATTTGAATCTGGTTATCAGTATGACTTGTGACTTGATATTATTGTATTTTCTGAACTTATTGGTGATTGGATATTTGAATGATTGTTTTGGTTTTGTATGAACTTTAAATTTTTTAGGNAA
TARGET=925,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0193
PRIMER_LEFT_PENALTY=0.016950
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=GGAAGAGGATAGCGAGAGTTCA
PRIMER_RIGHT_SEQUENCE=TCTTCTGTGCTCCCTCTTTTTC
PRIMER_LEFT=692,22
PRIMER_RIGHT=959,22
PRIMER_LEFT_TM=59.983
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=0.1163
PRIMER_LEFT_1_PENALTY=0.093026
PRIMER_RIGHT_1_PENALTY=0.023267
PRIMER_LEFT_1_SEQUENCE=AGAAAGAGAGGCAGAGCAAAGA
PRIMER_RIGHT_1_SEQUENCE=AGAATCTGACAGACGGCTTCTC
PRIMER_LEFT_1=849,22
PRIMER_RIGHT_1=1061,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=60.023
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.1163
PRIMER_LEFT_2_PENALTY=0.093026
PRIMER_RIGHT_2_PENALTY=0.023267
PRIMER_LEFT_2_SEQUENCE=GAGAAAGAGAGGCAGAGCAAAG
PRIMER_RIGHT_2_SEQUENCE=AGAATCTGACAGACGGCTTCTC
PRIMER_LEFT_2=848,22
PRIMER_RIGHT_2=1061,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=60.023
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.1163
PRIMER_LEFT_3_PENALTY=0.093026
PRIMER_RIGHT_3_PENALTY=0.023267
PRIMER_LEFT_3_SEQUENCE=AAGAGAGGCAGAGCAAAGAAGA
PRIMER_RIGHT_3_SEQUENCE=AGAATCTGACAGACGGCTTCTC
PRIMER_LEFT_3=852,22
PRIMER_RIGHT_3=1061,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=60.023
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=0.1980
PRIMER_LEFT_4_PENALTY=0.102490
PRIMER_RIGHT_4_PENALTY=0.095522
PRIMER_LEFT_4_SEQUENCE=TCAGGGAAGAAAGTTAGTGGGA
PRIMER_RIGHT_4_SEQUENCE=GGCTTCTCCTTCTCCTCTTCTC
PRIMER_LEFT_4=759,22
PRIMER_RIGHT_4=1047,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_04800 Ps_contig_mira-and-tgicl-ass_franssen_34658
SEQUENCE=AAAACTAATTTTTCATTTCAAACTCTAATCTATCTATCTCCCATTCTTGAAATTCTAAACATTTTCACATTCATTTCTTCGTTCATTCATTCACACTCAATTCAAGAAAACAATGGCAAGCACGCCGGTGAGAAAGCCACACACCAACACCTCCGATCTACTCACTTGGTCCGAAGTTCCTCCTCCGTCTGACTCCTCCGCCACCGTCTCCGCCGCCCGCTCTCGTCAGCCTTCTGATAGAATTAGCAAGGTTCTCAATAGCGACCAGCTCTCCGATGAAGAAGCTCTCACTCTCTCCAAAAGCAAGCCAATAGTTACARGGNATAATAAAAATAGAAAAGAGATGAGTGGAAATGGTATATTTTCG
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04801 Ps_contig_mira-and-tgicl-ass_franssen_34658
SEQUENCE=AAAACTAATTTTTCATTTCAAACTCTAATCTATCTATCTCCCATTCTTGAAATTCTAAACATTTTCACATTCATTTCTTCGTTCATTCATTCACACTCAATTCAAGAAAACAATGGCAAGCACGCCGGTGAGAAAGCCACACACCAACACCTCCGATCTACTCACTTGGTCCGAAGTTCCTCCTCCGTCTGACTCCTCCGCCACCGTCTCCGCCGCCCGCTCTCGTCAGCCTTCTGATAGAATTAGCAAGGTTCTCAATAGCGACCAGCTCTCCGATGAAGAAGCTCTCACTCTCTCCAAAAGCAAGCCAATAGTTACARGGNATAATAAAAATAGAAAAGAGATGAGTGGAAATGGTATATTTTCG
TARGET=82,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04802 Ps_contig_mira-and-tgicl-ass_franssen_34659
SEQUENCE=ACTCTTTGCACTANNATCTTCTTCTTTGATTTTACATTCAAATATTAGTAACCAAATTACCCTCTCTCTCTCTCTCTGCAATTTTCTTCCCCTTTACTTCTAGGTTTCGCTCCTTCTATGGGCGTGTCTTCCTCTACTCATCAGGTTTGAGAAGATAGCTTAGTTTGGTTTATTCACCAACTTCCATGCCCATGATGGACCACGGTAAAGATTTTCTCAGCTGGGTTGACCCTGATACGTCTCTCAAAATTTTCAAGTGTCTGGATGATCCAGCAGATCTAGTCCGCGTTAGCTGTGTCTCACGATCTTGGAGAGGCTTTGTGGTTGCTAACGGCCTTTCTAAGCAGTTGTGCTTGAGAATGTTTCCTCAATTATCTAGAGTTGCCTGTGTTGTTGAACTAAACCAGGACGGAGTGAAGGAACACACAGAGGTTGGATCTAGCCATTCTAATGAATGTCTAGCTCTATGGAAAGATCATAGAGTCTATAGTTATCTTGCTAGATCCTGCTCATCTCCTGTTGATACGGATTGCATAGCTAAGGCAATTGGTGCTTCTAGCACTGATAATTTCCCTCAGGAGAGTATTGATCATACTCTAGAGGAAAGAGACAACATTGCTGGTAGATTTTCCTATTGGTCGAGTAGCGGACAAAGCAATCCCAATGTGCCTGAGACATTAACATACCAGCTGGCTTCTCAAATATGTGTTATTACAGAAATCAATATTCAGCCTTTCCAAGCTCACTTTCAGATGGGGTCACCAATATATTCAGCAAAATCTGTTAGGTTTAAAATGGGTCATCTTAAAGCCTCTTTAGATGATTTAACTGATGTGTTTGTATGGACCTATACATCACCGGAGTTTCCAATGGCTCAGGAGAACCGGCTACAGAAGTTCAAGCTTCCAGAACCTGTTCTCTGTATTGGTGGTATCTTGCAGATTGAGCTTTTGGAAAGGGTGCAGAGCCAAGAAATGGATGGCTTATTATACATTTGTATTTCTCATGTTCAAGCTCTAGGGATTCAGTTGTCACCAGCATTTTCTGTTGATATTCTTGGGCCCTCTGGGAAGTTTGTGTTGAAGAGAGACCACATGGGTAACTGTCAACCCCCAGTGACTTCTGAAAATGAGTCTGGAGCAGCATTAACTGATAGAGATTTCCAACAGATTGTGACTATATTGCGGGGACATGTAATGGGAGTAGGAGCCGTGGATGGTTGGGAAGAGATGGAATATGAAGTTGATGATTTTGATGAAGATAATGCCTTCTGATCTTTGCTGGCATAAACTGTGACTATCCCCGCCTTCTCAAAACAATAAAGGAGACAAGAAAATTTAAGATTCTTCGGGTGGGGAGTCGTTGTAATATAACCAGTGTGATGTATAATACCCTTTTGTGTAACCTTTTAAAGTTCAGGTTTTTGTGTGATTTTGCTACTAATTTGTGTGATAAGTATTACATTTGAAGCTGTTTAAATTAAATTCATATGGTGTAGTTTAACTCCTCTTCTTTTGTTAGAGCTAGTTGGGATTTTCGCCCTCAAATCTGTTTCGATGTGTCATGCGCAATACATCGAAACATGACGATGGGTTGGTCTGTCAGTCTCATTTTTATTTAGCTGATTTATTGTTGTTTTGTCCARTTTTTAGACTATTTTTCTAAATATTGAAACTTTGTAAT
TARGET=62,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04803 Ps_contig_mira-and-tgicl-ass_franssen_34660
SEQUENCE=GAACATCTTTAGGAAACGGTACTGCACCCAGCAANCAAAAACTCATCAATTTGATCCCACCATCTGAACTCAGCTTCCATTTTATTACGAAGCACGTTATACAAAAGTGTAGGATAAAACAGAATCCGTGCACCTGCTCCTACCAACGCTCTCTTCGCATCAACCCTAACAATTTGTCTCTCCAAGCTCTCGTCTTCTACCCTACTACACTCAGCATCATCCAATTCCTCGATCTTCATCGATAACAAAACCTAAAAACCTTATTGGTGGAATTGGAATCAAGTGACGCAAAATCGAAAATTAGCGATAAATGGAAAATTGATGTGAGAGGAGAAAAAGGAATTGGGAACGACGGTAATCGAAGTGCTCACAAGAAGAAGAAG
TARGET=372,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_04804 Ps_contig_mira-and-tgicl-ass_franssen_34664
SEQUENCE=ACCTCACCCTCTTGTTGTTGTTTCTTATCCGAATGGAAGGGGAAGAACATAAGAAACACCGCCCCTTTATCCCCTGAATTAACTAACCGTCACTCTGTTCTGTTCCGCCCTATGAAACCTCCCAATTACATCTCTCATCCTTAAACTAACTAACCATCCTTGGAGAGGGGGGAGAATGTCTTGTTTATTGCCGCACTTTAACACTTTCACAACTCCTATCCCTATCCCCATCTCTACACACCGCCATCATCATATCATTCTTCAAACTAAATCAAGACGCAATTTCTATTTACGCCCGCACTGTATTCTGTCTCCTTCCGCACCATCAACCGCACTCCTTGAGTTCGATACTAATCAATTTCATGCTGCAAACACACCTTGGCCCTATGTAGCTTCTCAGGAAAACTATAAAGCAGCCACTTCTGCGGAATCACTTCTTGCCAGTGAAGACGCCGTTATAGCTGCTGCTGCTTCTGAAGCGATTGCTCTCGCTAAAGCTGCGGCAAAACTTGCAAAGGATGCCGCCTTATTAGTAAAACATAATTCCCCACAACAACAACCGGTGGTTAGGCCACAGCCTCCTTCCAAGCCTGAAAATTTACTTCTCAAATGGCTTCAACACATGGAAGCACAAGATGCTGTAGCAGAAGGACCACAGATAATGGAAGATGTTGACACTAGCTCAACTCAAGAGGAGGAGTTGGAGGATGAGGAACCTACCTCTGAGGAACTTGAAGTTGCCGAGGAAGAACTTTCCGATAGTATAGATGCAAGATCGGGCCGGCAGACAGAAAGAAAAGCCAGAAGAGTCAGGGCATGTGGGAAGGCTGCCACTAATATCGTGTCCTTTAAGTCTGGATCCATCAGTAGAAAAAAGCGTGTTCGGACACAAGAGATTGACTATTCTGATCCACTTCGTTACTTAAGAAATACAACCAGATCTACAAAGCTTCTTACTATATCTGAAGAAGTTAAGTTGTCTGCAGGAATACAGGATCTGATAACGCTCGAAAGACTACAAGAGGATCTTGCAGAAAAATCTGGTGGCAAGCCCACATTTTCTCAATGGGCTACAATGGCAGGGGTAGATCAGAAGACCCTAAGAAAACGATTAAATTATGGTGTATTTTGCAAAGAAAAAATGGTTAAAAGCAATATACGACTTGTCATATCAATTGCTAAGAATTACCAGGGATCTGGGATGAGTCTGCAAGATCTAGTCCAGGAAGGGTGCCGCGGTCTTGTAAGAGGTGCAGAGAAGTATGATGCTTCTAAGGGCTTTAAGTTCTCAACCTATGCTCATTGGTGGATTAAACAGGCAGTTCGGAAATCCCTTTCTGCCCAGTCTAGAATTATTCGCTTGCCATTCCACATGGTGGGGGCAACTTACAAAGTGAAGGCGGCACAGAAACAGTTGTACAGTGAAAATGGAAGACAACCAGACGATGCAGAAGTTGCTGAAGCAACTGGGCTGTCAATGAAGAGGCTTAATGCTGTTTATATGACCCCAAAAGCTCCCAGATCTCTAGAACAGAAAATTGGGATCAACCAGAGTCTTAAACCTTCAGAAGTACTTGCTGATCCGGAAGCTGAAACACCTGAAGAACAACTCATTAAGCAGTTCATGAAAAAGGACCTAGAAAAGGTACTAGACAGCCTGAATCCAAGAGAGAAACAGGTGATTAGATTGAGATTTGGTATGGACGATGGAAAGATGAAGACACTGCAAGAGATTGGGGAAATAATAGGCGTGAGTAGGGAGAGAGTTAGACAAATTGAGTCATGTGGTTTTAGGAGGCTTAAGAACAAGAAGAGAGCCAAACATTTGGAGCATTATTTGAATTTATAACCCTGCCAAATATTGATGTTAGGTTATTCAGTCCTGATATCTTTGTTCTTTACATGTATGTTGTTCCTCTACTTTTTCTTCATTATTCAAGAGAAAACAGAAAGGTTGTTTCAGTATACTAGGAATCAGGAAATCAATGTAAACACAAGCAAATAACTTTTGACCAAGAGTTTCCCATTTTTTAATGTAAGCGATTGATTCGAAACC
TARGET=461,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1966
PRIMER_LEFT_PENALTY=0.122569
PRIMER_RIGHT_PENALTY=0.073989
PRIMER_LEFT_SEQUENCE=GCCCTATGTAGCTTCTCAGGAA
PRIMER_RIGHT_SEQUENCE=CCTTCTGCTACAGCATCTTGTG
PRIMER_LEFT=381,22
PRIMER_RIGHT=650,22
PRIMER_LEFT_TM=59.877
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=270
PRIMER_PAIR_PENALTY_1=0.2093
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.086698
PRIMER_LEFT_1_SEQUENCE=GCCCTATGTAGCTTCTCAGGAA
PRIMER_RIGHT_1_SEQUENCE=GGTCCTTCTGCTACAGCATCTT
PRIMER_LEFT_1=381,22
PRIMER_RIGHT_1=653,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.2661
PRIMER_LEFT_2_PENALTY=0.122569
PRIMER_RIGHT_2_PENALTY=0.143555
PRIMER_LEFT_2_SEQUENCE=GCCCTATGTAGCTTCTCAGGAA
PRIMER_RIGHT_2_SEQUENCE=GGTTGTTGTTGTGGGGAATTAT
PRIMER_LEFT_2=381,22
PRIMER_RIGHT_2=560,22
PRIMER_LEFT_2_TM=59.877
PRIMER_RIGHT_2_TM=59.856
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.3412
PRIMER_LEFT_3_PENALTY=0.122569
PRIMER_RIGHT_3_PENALTY=0.218662
PRIMER_LEFT_3_SEQUENCE=GCCCTATGTAGCTTCTCAGGAA
PRIMER_RIGHT_3_SEQUENCE=CTGCTACAGCATCTTGTGCTTC
PRIMER_LEFT_3=381,22
PRIMER_RIGHT_3=646,22
PRIMER_LEFT_3_TM=59.877
PRIMER_RIGHT_3_TM=60.219
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=0.3412
PRIMER_LEFT_4_PENALTY=0.122569
PRIMER_RIGHT_4_PENALTY=0.218662
PRIMER_LEFT_4_SEQUENCE=GCCCTATGTAGCTTCTCAGGAA
PRIMER_RIGHT_4_SEQUENCE=TCTGCTACAGCATCTTGTGCTT
PRIMER_LEFT_4=381,22
PRIMER_RIGHT_4=647,22
PRIMER_LEFT_4_TM=59.877
PRIMER_RIGHT_4_TM=60.219
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_04805 Ps_contig_mira-and-tgicl-ass_franssen_34676
SEQUENCE=AAAAGTAAGCAATAGCAGTAAGATAGAAAGAAAGAAAATGGGTTTTTATTCACTTCACTAACACCCCACAACAATCAAGTTACATAGAAGAAAACACATTATTCTCAGTCTCACACTGATCAATCTAACAAATGGATAAGAATTGCTGATAAATAAAAATCTCAAAAACAGAGGTTTTACAGAGTGGAACCAGTCCCCATGTTTTACAGGTACTAGTCCTTTCTCAAACAAAAGTAAGGCACGGTTCACCAGAAGCTATGCCAAATTCCTACCATGGACGCTTCAAATGGACCCCCAATCTCTTCAATAATGCTCAGTTGGCGACCCAGAAGCATGAAATTCATGAAAGAGAAACAGAAGCAGCATGTTTGATCTTCACTCAACTTCAAGCATTGCAAATATGCATATCACACTAATCCATGCTTCCATTTCCCTTTTCCAACAGAAACCTCAATAATCTAAACATATATTTCATCATCTTCTAAAAACCTAATCAGTTTTCCCACCTAGGAAAACCCTAATTCTAACTGAATTCCTCAATTCAAGGACTTCTCCACTCCAAAATTAACATAAACCTAACCCTAAAATTTGACTAAGAATAAACGTAGCTATAAAATTAACCACACACAGACACCATAATTAATACATAGCTATAAATATATAGTAAGAACGTGAACAGTGAAAACCAGAGTAATTTTTCTCCAATCAACGATCCTCATCGTCTCCCCGGCCGGAGTTTCCATTACCACCTGACAATGAAGCCAGCAAATTCAGATGTCCGGGAAGATAATTCCCTAGCCTCGCCGCGGAAGCTTCTCCCATAGAACCTTGTTGTTGATGTTGCTGCTGTTGTTGCTGTTGCTGCTGATGATGGAACAGCGAACTATGCTGATGCTGATTGGGAGAAATGGAAAGAACATCCGGCGGAGGTTGAGCCGCCGCAGCGAAACTCCAAAGCTGAGCGAAATCCGGCCTCCATAAAGCAGGAGGAGCAGTAGGAGCCGCAATCGAAACATCGTCGTGGTCGGAACGTAACCGTTTACCGAGAATGAAGGGAGAAGGAGAGAGAAACGGTTTATGCTCGGAGAGAGAAGAGAAAGAAGCGGGAGTAGTTCCGGTACCAGTGGCGGCGATGATGGAGGGCTCGGCTTGACGGAGAAGCCATTCGATGGTTTGACCGTCGGATTTATGACCGAGCTCACGTGTTAACTGGAAGACACGTGCGGCGCATATTATTGGCATGCGTATTCTTCGACCGCGTCCGTCAACTTTGCTGTGACGGTCTTTTGACGGTGGTTTTTTAACGGTTATGGCGGTTTGGTTTGTGAGTTCTGGGTCTGTTGTGACGGCGTTAGATGTTTCCTTGGAGTTAGACATGTGAAATAAATTTGGGGTTTGGGGTTTTTTTGAATTTAATATTGTGAATGAAAGGGTTTTGGTTTGGGTTTTTTGATTGTTTAGAGGAGGAGGAAGGAGGAGGAGGGAGTGAGTAATGTAATGATTNAGTTGAACAGGAGTGGTGANANAGGAAGAGCANAGTGGGTGG
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04806 Ps_contig_mira-and-tgicl-ass_franssen_34676
SEQUENCE=AAAAGTAAGCAATAGCAGTAAGATAGAAAGAAAGAAAATGGGTTTTTATTCACTTCACTAACACCCCACAACAATCAAGTTACATAGAAGAAAACACATTATTCTCAGTCTCACACTGATCAATCTAACAAATGGATAAGAATTGCTGATAAATAAAAATCTCAAAAACAGAGGTTTTACAGAGTGGAACCAGTCCCCATGTTTTACAGGTACTAGTCCTTTCTCAAACAAAAGTAAGGCACGGTTCACCAGAAGCTATGCCAAATTCCTACCATGGACGCTTCAAATGGACCCCCAATCTCTTCAATAATGCTCAGTTGGCGACCCAGAAGCATGAAATTCATGAAAGAGAAACAGAAGCAGCATGTTTGATCTTCACTCAACTTCAAGCATTGCAAATATGCATATCACACTAATCCATGCTTCCATTTCCCTTTTCCAACAGAAACCTCAATAATCTAAACATATATTTCATCATCTTCTAAAAACCTAATCAGTTTTCCCACCTAGGAAAACCCTAATTCTAACTGAATTCCTCAATTCAAGGACTTCTCCACTCCAAAATTAACATAAACCTAACCCTAAAATTTGACTAAGAATAAACGTAGCTATAAAATTAACCACACACAGACACCATAATTAATACATAGCTATAAATATATAGTAAGAACGTGAACAGTGAAAACCAGAGTAATTTTTCTCCAATCAACGATCCTCATCGTCTCCCCGGCCGGAGTTTCCATTACCACCTGACAATGAAGCCAGCAAATTCAGATGTCCGGGAAGATAATTCCCTAGCCTCGCCGCGGAAGCTTCTCCCATAGAACCTTGTTGTTGATGTTGCTGCTGTTGTTGCTGTTGCTGCTGATGATGGAACAGCGAACTATGCTGATGCTGATTGGGAGAAATGGAAAGAACATCCGGCGGAGGTTGAGCCGCCGCAGCGAAACTCCAAAGCTGAGCGAAATCCGGCCTCCATAAAGCAGGAGGAGCAGTAGGAGCCGCAATCGAAACATCGTCGTGGTCGGAACGTAACCGTTTACCGAGAATGAAGGGAGAAGGAGAGAGAAACGGTTTATGCTCGGAGAGAGAAGAGAAAGAAGCGGGAGTAGTTCCGGTACCAGTGGCGGCGATGATGGAGGGCTCGGCTTGACGGAGAAGCCATTCGATGGTTTGACCGTCGGATTTATGACCGAGCTCACGTGTTAACTGGAAGACACGTGCGGCGCATATTATTGGCATGCGTATTCTTCGACCGCGTCCGTCAACTTTGCTGTGACGGTCTTTTGACGGTGGTTTTTTAACGGTTATGGCGGTTTGGTTTGTGAGTTCTGGGTCTGTTGTGACGGCGTTAGATGTTTCCTTGGAGTTAGACATGTGAAATAAATTTGGGGTTTGGGGTTTTTTTGAATTTAATATTGTGAATGAAAGGGTTTTGGTTTGGGTTTTTTGATTGTTTAGAGGAGGAGGAAGGAGGAGGAGGGAGTGAGTAATGTAATGATTNAGTTGAACAGGAGTGGTGANANAGGAAGAGCANAGTGGGTGG
TARGET=1472,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9079
PRIMER_LEFT_PENALTY=0.185267
PRIMER_RIGHT_PENALTY=1.722639
PRIMER_LEFT_SEQUENCE=TTTGTGAGTTCTGGGTCTGTTG
PRIMER_RIGHT_SEQUENCE=TCACCACTCCTGTTCAACTNAAT
PRIMER_LEFT=1321,22
PRIMER_RIGHT=1522,23
PRIMER_LEFT_TM=60.185
PRIMER_RIGHT_TM=59.277
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.5500
PRIMER_PAIR_COMPL_ANY=4.75
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=1.9079
PRIMER_LEFT_1_PENALTY=0.185267
PRIMER_RIGHT_1_PENALTY=1.722639
PRIMER_LEFT_1_SEQUENCE=TTTGTGAGTTCTGGGTCTGTTG
PRIMER_RIGHT_1_SEQUENCE=CACCACTCCTGTTCAACTNAATC
PRIMER_LEFT_1=1321,22
PRIMER_RIGHT_1=1521,23
PRIMER_LEFT_1_TM=60.185
PRIMER_RIGHT_1_TM=59.277
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.5750
PRIMER_PAIR_1_COMPL_ANY=4.75
PRIMER_PAIR_1_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=1.9195
PRIMER_LEFT_2_PENALTY=0.196906
PRIMER_RIGHT_2_PENALTY=1.722639
PRIMER_LEFT_2_SEQUENCE=GTGAGTTCTGGGTCTGTTGTGA
PRIMER_RIGHT_2_SEQUENCE=TCACCACTCCTGTTCAACTNAAT
PRIMER_LEFT_2=1324,22
PRIMER_RIGHT_2=1522,23
PRIMER_LEFT_2_TM=60.197
PRIMER_RIGHT_2_TM=59.277
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.5500
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=1.9195
PRIMER_LEFT_3_PENALTY=0.196906
PRIMER_RIGHT_3_PENALTY=1.722639
PRIMER_LEFT_3_SEQUENCE=GTGAGTTCTGGGTCTGTTGTGA
PRIMER_RIGHT_3_SEQUENCE=CACCACTCCTGTTCAACTNAATC
PRIMER_LEFT_3=1324,22
PRIMER_RIGHT_3=1521,23
PRIMER_LEFT_3_TM=60.197
PRIMER_RIGHT_3_TM=59.277
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.5750
PRIMER_PAIR_3_COMPL_ANY=4.75
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=1.9195
PRIMER_LEFT_4_PENALTY=0.196906
PRIMER_RIGHT_4_PENALTY=1.722639
PRIMER_LEFT_4_SEQUENCE=TGAGTTCTGGGTCTGTTGTGAC
PRIMER_RIGHT_4_SEQUENCE=TCACCACTCCTGTTCAACTNAAT
PRIMER_LEFT_4=1325,22
PRIMER_RIGHT_4=1522,23
PRIMER_LEFT_4_TM=60.197
PRIMER_RIGHT_4_TM=59.277
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.5500
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=198
=
PRIMER_SEQUENCE_ID=SSR_04807 Ps_contig_mira-and-tgicl-ass_franssen_34679
SEQUENCE=CACNCTCTAATCCCACCANCNCANCTCTCCGCTACCCAACCTCAATTNAAACCTAACCCAAACACTTTCAATTCCCTTCCCTTCCCACTTCTCTTRTTCTCTCTTTTCTTCCTCTTGATCTTCTCCCTCTCCGCCCTCGCCACCATCACCCACAGCGTCTTCCACGGCTTCTTCGGCCGTCCCGTCAAACTTCTCTCTACCGTCACCTCGCTCTTCTCCTCCTTCCTCCCTCTCCTTCTCACCTCCATCCTCTCCCTCCTCATCCTCTTCTCACTCTCCCTCCCTATCCCCCTCCTCTCTCTCCTCCTCCGCCCCAACCTCTCTTCCTCCCCAATCACCCTCACCGCCTCCGCTATCCTCCTCCCCGCACTCATCTTCTTCCTCTTCTACCTCCGTATCTCATGGACTCTCGCCTCAGTCATCGCAGTCGTCGAAAACACCTACGGAACTCAACCTCTCCGCCGCAGCGCCGCTCTTATGGATGGAATGAAATGCACCGGAGCTTCTTGCTTTCTGTTCTTCGCTTCTCTGGAAGGAATCATACTATGGAGTGGCTCACTTCTCGCAGCAGTGGCTTCTGATAGCGGTTCATGGAAGGATTGGGCTTTTGTGGTTCAGATTGTGTTGACTTCAGCCGTCTTGACTATACTTATGCTTTACAAGGCCGCTGCAAACACCGTCTTGTACATGTACTGTAAGGCGGTTCACGGTGAACTGGCGTTGGAGATCGCGGAAGAGTTTGCGTGGCAGTATGTGAGTTTGCCTTTTGATGAAAGCAAAGTCCCTCATGTTGTGTCTGTCGTAAGTGTAGTAAAATGTAAATGTTTGGTGGTTTGATTATAGGTAAAAAAATCATGTTGGTTTAGATTGTGCTAAATATTTGTGACATATCAAATGTGTAAGATGTGGATACTATTGCTTATTGATTATGAGCCGCAAATGTGATTT
TARGET=72,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04808 Ps_contig_mira-and-tgicl-ass_franssen_34682
SEQUENCE=TTTGTTCAAAAGCAGCCCACTGTTTGCTTCATTTCAAACCAAGTTTGTAAAGAAAATATGGATTTGCAGATGTCCAACTGAAATTCAAGAATGCAGCCTTGCAAGAAACATTGGCGGCATCAAGAACAAAGAACAAATATTTCTATCAGACTAAAACAGCTTAAACCTATAAAATCGACAAGTTTTTTCCAGCAACGGAATTATGTAGGCCAAAAAATGATATGGTTCAAGGCACGAAAGAAAATCTACATTTTATCTTCAGTTTAATCACACCTCAATCTGCAGTGGCTTCAAAAGTCTTTGGTGAACTGACAAATCAATTATCATGAAACTGACAATCACTCTTTCTTTGGGTCATCTAATTCAGTATTGTCATTGAATCATTTCCAGAAGCGTTTCTGTCCTTGCGATTTCCTCATATGCTCTTTGGAACGCCTAATGAATTCACTTGGAATATCAGATCCTTGGTAAGACTGTAGCACCGGTCTTATATCAGCCGGACTACTACGGGCAACAAACTCAAGGAATGGTATGAAATCCACAAGAGCTGTATCATCTTTATCAGTCTGCAGCCATGGCTTAATAGGGACACAGTTTTCAGGTTGTAGGCAACTTTCAAGAGCATGGCCACTTAAGTACATAACTTTTGCAGGGTTCCTGTTTAGTTTTGTAAGATCTCTATAGTGTTTCCCATCCTGATACTTAGTAGCAGGCCTTGATAGCCTGTACCTAATGCAGTGCTTGGGATCCAACCTCTCTATAACAGGGTCTACAAACATATTTTGTTCATCTGTATACACTACTATTTCATAGAACTGAGCTAGATGTTCTAAGAAAGCATCAACTCCAGGTCTTTTAAAAGTCTGCCAGCCTGTATCTCGCGTCCAAATGTAGTGAATCAATGTTTCATTGAGATCTAGAACAAGTGTGAACACATGTTGCTCTTGAGGAAGCAAATCTGGAAGGAGCTTGTCTGTGTATGGCTCTGTATAACTCCTAACTTGTTCTTCAATTAACCTTCTTGTGTCCAAATAAAGCTCTATTGCTTTAGCAGGCACTGTTACTGCAGTTGAGTATACCAGTCCTTGAAATTTATCAAGAGCGGTATCTCCATCAGCTGGAGTGTTCTTAGCCGATTGACGAAACAACTTCGTTTTCTCATCTATTTCGTCCGCACTATAAGCGTAAGAAGCATAGCCAGTAAGGAGAGTAGTTCCGGTGAAAGCCCCGATGACGGCGTATTTGAGGAAATTCCAGCGGTTAGGGGAAGTGGCGGAAGAAGGAGGAGGAGGTTGAGGCGCAGCGGGAGCTGCATTGGTGGAGAGGAATCGCTTCGATGAGGATGAAGCTACGGAGGAGAATCGAGATCGGAGAATTCTGAGAGACATGGTTGAGAAGGCGATGAAATGTAGTGAATGGGGTTCAAGTAGGGTTTTGGGTTGGCTTTGCAGTGACGCCGATCAACGCTGTGCTCTGTTTGGCTTTCTGT
TARGET=1281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2601
PRIMER_LEFT_PENALTY=0.086698
PRIMER_RIGHT_PENALTY=0.173391
PRIMER_LEFT_SEQUENCE=ATCAGCTGGAGTGTTCTTAGCC
PRIMER_RIGHT_SEQUENCE=CAGAATTCTCCGATCTCGATTC
PRIMER_LEFT=1112,22
PRIMER_RIGHT=1379,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=60.173
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=0.2910
PRIMER_LEFT_1_PENALTY=0.276069
PRIMER_RIGHT_1_PENALTY=0.014945
PRIMER_LEFT_1_SEQUENCE=CGCACTATAAGCGTAAGAAGCA
PRIMER_RIGHT_1_SEQUENCE=AAAACCCTACTTGAACCCCATT
PRIMER_LEFT_1=1172,22
PRIMER_RIGHT_1=1435,22
PRIMER_LEFT_1_TM=59.724
PRIMER_RIGHT_1_TM=59.985
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.3094
PRIMER_LEFT_2_PENALTY=0.135999
PRIMER_RIGHT_2_PENALTY=0.173391
PRIMER_LEFT_2_SEQUENCE=GTTCTTAGCCGATTGACGAAAC
PRIMER_RIGHT_2_SEQUENCE=TCAGAATTCTCCGATCTCGATT
PRIMER_LEFT_2=1124,22
PRIMER_RIGHT_2=1380,22
PRIMER_LEFT_2_TM=60.136
PRIMER_RIGHT_2_TM=60.173
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.3094
PRIMER_LEFT_3_PENALTY=0.135999
PRIMER_RIGHT_3_PENALTY=0.173391
PRIMER_LEFT_3_SEQUENCE=GTTCTTAGCCGATTGACGAAAC
PRIMER_RIGHT_3_SEQUENCE=CAGAATTCTCCGATCTCGATTC
PRIMER_LEFT_3=1124,22
PRIMER_RIGHT_3=1379,22
PRIMER_LEFT_3_TM=60.136
PRIMER_RIGHT_3_TM=60.173
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.3109
PRIMER_LEFT_4_PENALTY=0.137489
PRIMER_RIGHT_4_PENALTY=0.173391
PRIMER_LEFT_4_SEQUENCE=GGAGTGTTCTTAGCCGATTGAC
PRIMER_RIGHT_4_SEQUENCE=TCAGAATTCTCCGATCTCGATT
PRIMER_LEFT_4=1119,22
PRIMER_RIGHT_4=1380,22
PRIMER_LEFT_4_TM=60.137
PRIMER_RIGHT_4_TM=60.173
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_04809 Ps_contig_mira-and-tgicl-ass_franssen_34699
SEQUENCE=CACACCAACCAAACCCTACCTTATTATTATTACCTACATATATATGATTCAACATCTACATGTCCCTAACATGGAAATAGTTCTTGTTCTTAACGACAATGTCATACATGTGCATGGGCTTCAGCTTGTTGAAATCCTTTGGAAGAGAAATAAGGTACACGGTGGAGCGTTTGTGGAACTGAAGCAGATGATCAGGGTCATCGTAGTAGCGTTCCCAACCAAGAGAGCTTAGTTTTCGTTCCAGCATAGAATACGAAGTGATTACTTCATTGGTTGGGGTGTGTACAAGCACTTTTCTCCTTCCACTATTCTCACCCCCCTCGCCTCCTGGATTCTCTACTAGCCTCACCACCCCGTTCTTAAATACCYAAACCCCTGACATCAATTTATGTATACACAAAACAAACACTAAGAAGAAAGATATATGTTCTTGTTTTTGTGTGTGTYTTTGCAAACTTCC
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04810 Ps_contig_mira-and-tgicl-ass_franssen_34704
SEQUENCE=CTGTGCCCACAACACAACTGCATATAACCTGCGTCTGATATAAACRAGGTTTGAACACACTTTATCACATATATCATATCATGTACTATCATCATCATCATCTCTTCCTCTTGTTTTTTTCACCATGATTTCAGGGTCTTCTTCCATATTCCATTCTCTCTTTTTCCCCTAGTTTTTCTCCATAAAAATCTCTTTTTTACCTCCTCAAATAGAATAAAATTATCATTTTTTCCAACATCAGATACCCCATTTGGATACTTCACTCTGTCAAGGATTATTTGTTGATTGAATTCTAAAGTGTAATTGAAAAATGGAAGGTGATATATTCGGAGAACTAGGCGAAACGAGTCAAGTAGATAGTAGGCTTTTACAGGTATTTCAGAAGAGTTTACTGCAAGCGCAAGATATTTTGAATCAAAACCGGCTTTTAATCAATGAGATAAACCAGAATCATGAGTCTAGGATGCCTGATAATCTGAGCAGAAATGTCGGTTTGATTAGAGAGCTCAATAGCAATATCAGAAGAGTGGTTGATCTTTATGCTGATCTTTCGAGTTCTTTTACCAAGTCGCAAGAAGCTTCGTCTGAAGGGGACTCGAGTGGAACTCTAAAATCTGATGGAAAAGTCAACCAGAAGAGAATTAGATCAAGTTAACAGGTTGAAATTCAAGGTTTCGTGAGGCAGTGAAGTGAAGAAGTTTATTCTGATGCTGAATGAATGAATTTTTCTGGTGAGACTATGTGTAACTCGTCCAAGGTTTCTTCTGCGCGATTTCTTCCGTATTGGTTTTATGTTTAAAAAGGCTCATAGCTTTAGTTGATAATCTAATTAATGTAGCATCAATTTGAGGACTTCACAGTTGTGATTTTCTGTAACACTGTTAGCTTCATGAAATGAAACATGTTCTTGTGGACAAGTTAAATCAAATTTTCGTGATTGAAACTTAAACGT
TARGET=88,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04811 Ps_contig_mira-and-tgicl-ass_franssen_34704
SEQUENCE=CTGTGCCCACAACACAACTGCATATAACCTGCGTCTGATATAAACRAGGTTTGAACACACTTTATCACATATATCATATCATGTACTATCATCATCATCATCTCTTCCTCTTGTTTTTTTCACCATGATTTCAGGGTCTTCTTCCATATTCCATTCTCTCTTTTTCCCCTAGTTTTTCTCCATAAAAATCTCTTTTTTACCTCCTCAAATAGAATAAAATTATCATTTTTTCCAACATCAGATACCCCATTTGGATACTTCACTCTGTCAAGGATTATTTGTTGATTGAATTCTAAAGTGTAATTGAAAAATGGAAGGTGATATATTCGGAGAACTAGGCGAAACGAGTCAAGTAGATAGTAGGCTTTTACAGGTATTTCAGAAGAGTTTACTGCAAGCGCAAGATATTTTGAATCAAAACCGGCTTTTAATCAATGAGATAAACCAGAATCATGAGTCTAGGATGCCTGATAATCTGAGCAGAAATGTCGGTTTGATTAGAGAGCTCAATAGCAATATCAGAAGAGTGGTTGATCTTTATGCTGATCTTTCGAGTTCTTTTACCAAGTCGCAAGAAGCTTCGTCTGAAGGGGACTCGAGTGGAACTCTAAAATCTGATGGAAAAGTCAACCAGAAGAGAATTAGATCAAGTTAACAGGTTGAAATTCAAGGTTTCGTGAGGCAGTGAAGTGAAGAAGTTTATTCTGATGCTGAATGAATGAATTTTTCTGGTGAGACTATGTGTAACTCGTCCAAGGTTTCTTCTGCGCGATTTCTTCCGTATTGGTTTTATGTTTAAAAAGGCTCATAGCTTTAGTTGATAATCTAATTAATGTAGCATCAATTTGAGGACTTCACAGTTGTGATTTTCTGTAACACTGTTAGCTTCATGAAATGAAACATGTTCTTGTGGACAAGTTAAATCAAATTTTCGTGATTGAAACTTAAACGT
TARGET=712,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04812 Ps_contig_mira-and-tgicl-ass_franssen_34710
SEQUENCE=AACGCAGAGNANGCGRGGAGCGCTAACACAGAGATGATGAAAACATGCGCATGCCTTCACACTTCCTCTTCTTCTTCACTACCATTATTACCATCAACATTTCTATTTCAACAACAACAACTACGCTATCATCATTTTCTCAATTTTCGCTCCTTATCTCTTAACGCTACACCGCGCCAAATTCAACTTCAACTCTCTTCCTCCAACGACGCCGTTTTATCACAAAACCCTACACCGCCGCACATACAAGAACCACCTCCTCCAACTCCACTTGTTCTCTCCGGTGAAGATAAAACCACTAATAATAATAATAAGCTTAAAAAAAAGAAAGACCTAAACGACGATAACAGTAGCAACTTCGATAACCGCTTCAAGCTCCGTAACGGAAAAGAGGTTTTTGAAGAGAAAGCTTATCTGGTTGGTGTTGAGCGTAAAGGTGATGTGAGTGATTCTTTTGGTATTGAAGATTCTTTGAGTGAATTGAAACAGTTAGCTGATACGGCTGGACTACTGGTTGTTGACTCTACATATCAGAAGCTTGCATCTCCAAACCCTAGGACTTACATTGGCTCCGGCAAAGTTTCTGAAATTAAGAGTGCAATCAATGCATTGGATGTTGAAACTGTGATATTTGATGATGAGCTATCAGCTGGGCAATTGCGCAACCTGGAAAAGGTTTTTGGGGGAGATGTGAGAGTTTGCGATCGAACTGCTCTCATCCTTGATATATTTAATCAGCGAGCAGCAACACATGAAGCATCTTTACAGGTTTCATTAGCGCAAATGGAATACCAATTACCTCGTCTAACAAAAATGTGGACTCATCTTGAGCGCCAATCAGGAGGACAGGTGAAAGGAATGGGTGAAAAACAAATAGAAGTGGACAAACGTATTTTGCGTAACCAAATTGGCGTTCTTAAAAAAGAGCTAGAATCTGTTAGGAAACACCGAAAGCAGTATCGTAATAGGCGTGTCTCAGTACCTGTGCCAGTGATATCTTTGGTCGGATATACAAATGCTGGTAAGAGTACGCTTTTAAATCAATTAACTGGAGCAGATGTTCTTGCTGAGGATAAATTATTTGCAACTTTAGATCCAACTACAAGGAGGGTACAGATGAAGAATGGAAAGGAGTTTCTCCTAACAGACACTGTTGGTTTTATTCAGAAGTTACCTACTACACTAGTTGCTGCCTTCAGAGCCACACTCGAAGAGATATCAGAGTCATCACTACTGGTTCATGTTGTTGATATCAGTCACCCCCTGGTTGAGCAACAGATAAATGCTGTTGACAAAGTTCTGTCAGAACTAGATGTATCATCAATACCAAGATTGATGGTCTGGAATAAGATTGATAAGGCTAGTGATCCCCAGAAAATCAGGCTAGAAGCTGAAAAAAGAGATGATGTTGTATGCATATCTGCTTTAAGTGGTGATGGCTTACAAGAATTCTGTGATGCAATTCAAGACAAACTAAAGGACTCAATGGTATGGGTGGAAGCTTTGTTACCATTTGAAAATGGGGACCTTCTCAGCACCATACACCAAGTTGGAATGGTTGAGAAAACTGAATATACAGAGCAAGGGACATACATTAAGGCTCATGTGCCCCTACGTTATGCAAGATTGCTTACACCTATGAGGCAACTGTGTGTATCACGACCTTGACTTTCTTACATGTGTGTATAATTTTATTTAACATTAACTTTCATATATCTGTCATTCGTTGGCGTAGAAAATGATTAATACAATTTAGCATCAGTTATGATTGAGGCCATGGGTGAGAAACACTTGGCTTGCAGCATAACAGGTCCCATGTAAAGCTGTACATATGTATGTAATTATTGAAGGACTGAGGTCATAATCAGATTAGCAAGCCCTTGAAAATGTTGACACCTTGAAGGATGGCAATGCAAAGTGTGTTTGCACTTTGCAACCACAGTTTATCATACATATTCAGCCCAATTGTTTTTAGTTTTTAAACAGTCGTTTAATTACTAA
TARGET=109,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04813 Ps_contig_mira-and-tgicl-ass_franssen_34710
SEQUENCE=AACGCAGAGNANGCGRGGAGCGCTAACACAGAGATGATGAAAACATGCGCATGCCTTCACACTTCCTCTTCTTCTTCACTACCATTATTACCATCAACATTTCTATTTCAACAACAACAACTACGCTATCATCATTTTCTCAATTTTCGCTCCTTATCTCTTAACGCTACACCGCGCCAAATTCAACTTCAACTCTCTTCCTCCAACGACGCCGTTTTATCACAAAACCCTACACCGCCGCACATACAAGAACCACCTCCTCCAACTCCACTTGTTCTCTCCGGTGAAGATAAAACCACTAATAATAATAATAAGCTTAAAAAAAAGAAAGACCTAAACGACGATAACAGTAGCAACTTCGATAACCGCTTCAAGCTCCGTAACGGAAAAGAGGTTTTTGAAGAGAAAGCTTATCTGGTTGGTGTTGAGCGTAAAGGTGATGTGAGTGATTCTTTTGGTATTGAAGATTCTTTGAGTGAATTGAAACAGTTAGCTGATACGGCTGGACTACTGGTTGTTGACTCTACATATCAGAAGCTTGCATCTCCAAACCCTAGGACTTACATTGGCTCCGGCAAAGTTTCTGAAATTAAGAGTGCAATCAATGCATTGGATGTTGAAACTGTGATATTTGATGATGAGCTATCAGCTGGGCAATTGCGCAACCTGGAAAAGGTTTTTGGGGGAGATGTGAGAGTTTGCGATCGAACTGCTCTCATCCTTGATATATTTAATCAGCGAGCAGCAACACATGAAGCATCTTTACAGGTTTCATTAGCGCAAATGGAATACCAATTACCTCGTCTAACAAAAATGTGGACTCATCTTGAGCGCCAATCAGGAGGACAGGTGAAAGGAATGGGTGAAAAACAAATAGAAGTGGACAAACGTATTTTGCGTAACCAAATTGGCGTTCTTAAAAAAGAGCTAGAATCTGTTAGGAAACACCGAAAGCAGTATCGTAATAGGCGTGTCTCAGTACCTGTGCCAGTGATATCTTTGGTCGGATATACAAATGCTGGTAAGAGTACGCTTTTAAATCAATTAACTGGAGCAGATGTTCTTGCTGAGGATAAATTATTTGCAACTTTAGATCCAACTACAAGGAGGGTACAGATGAAGAATGGAAAGGAGTTTCTCCTAACAGACACTGTTGGTTTTATTCAGAAGTTACCTACTACACTAGTTGCTGCCTTCAGAGCCACACTCGAAGAGATATCAGAGTCATCACTACTGGTTCATGTTGTTGATATCAGTCACCCCCTGGTTGAGCAACAGATAAATGCTGTTGACAAAGTTCTGTCAGAACTAGATGTATCATCAATACCAAGATTGATGGTCTGGAATAAGATTGATAAGGCTAGTGATCCCCAGAAAATCAGGCTAGAAGCTGAAAAAAGAGATGATGTTGTATGCATATCTGCTTTAAGTGGTGATGGCTTACAAGAATTCTGTGATGCAATTCAAGACAAACTAAAGGACTCAATGGTATGGGTGGAAGCTTTGTTACCATTTGAAAATGGGGACCTTCTCAGCACCATACACCAAGTTGGAATGGTTGAGAAAACTGAATATACAGAGCAAGGGACATACATTAAGGCTCATGTGCCCCTACGTTATGCAAGATTGCTTACACCTATGAGGCAACTGTGTGTATCACGACCTTGACTTTCTTACATGTGTGTATAATTTTATTTAACATTAACTTTCATATATCTGTCATTCGTTGGCGTAGAAAATGATTAATACAATTTAGCATCAGTTATGATTGAGGCCATGGGTGAGAAACACTTGGCTTGCAGCATAACAGGTCCCATGTAAAGCTGTACATATGTATGTAATTATTGAAGGACTGAGGTCATAATCAGATTAGCAAGCCCTTGAAAATGTTGACACCTTGAAGGATGGCAATGCAAAGTGTGTTTGCACTTTGCAACCACAGTTTATCATACATATTCAGCCCAATTGTTTTTAGTTTTTAAACAGTCGTTTAATTACTAA
TARGET=300,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04814 Ps_contig_mira-and-tgicl-ass_franssen_34711
SEQUENCE=CGCAGAGTACGCGGGGGTTGTCTCTCTCTCTTCCCTCACACATCGCACAACACAGGCTCAACTTCTCCGTTCCGTTGTGTTGTGTCGTGCTGTATTGTGCTGTGTCTTCTTTCTTCTTCTTCATTCTCTCACTATCACGCTAACGACCCTATTCTCAATTCCATCCAACAAACTCTGCATTTTCCATTTCTTTTTTTCCCTAAATCAATTACACTAATGCTTCTTCGTCTTCCTCTCTCATCAACAACCTAGGGTTTCTCTCCGATTTTCCTTTCATTTCGCCCGGATTTTGGGGTTAAGCTCAATCTCCGGCTTCCATGGCTTCCAACTTGAACATGACTCCTAAGCTCGAACAGATCCACGGTGAAATTCGCGATATCTTCCGAGCCCTTGCAAATGGCTTCCAGAAGCTGGATAAGATTAAAGATTCCAATAGACAAAGCAATCAGCTGGAGGAACTCACTGGGAAGATGAGGGATTGCAAAAGGTTGATAAAAGATTTTGATCGTGAAGTTAAAGATGAAGGGGCAGGAAATCCCGAAGAAGTGAATAAGCAACTCAATGACGAAAAGCAATCAATGATCAAGGAGCTAAACTCATATGTGGCACTGAGAAAAACGTACATGAATACCATTGGTAATAAAAAACTTGAACTTTTTGACATGGGAGCTGGTGCAAGTGAACCCGCTGAAGGAAATGTTCAACTCGCATCAGAAATGTCAAATCAAGAACTTGTCAATGCTGGGATGAAGACAATGGACGAGACTGATCAGGCTATTGAGAGGTCTAAGCAGGTTGTACATCAAACAATTGAAGTTGGCACCCAAACTGCTTCTACCTTGAAGGGCCAAACTGATCAAATGGGTCGTATTGTTAATGAGCTGGATTCAATTCAGTTTTCAATTAAGAAAGCCTCCCAACTTGTTAAAGAGATCGGTAGACAGGTAGCTACAGACAAATGCATCATGCTGTTTCTGTTTCTTATCGTCTGTGGTGTAATAGCCATTATTGTCGTGAAGATTGTGAATCCCAACAACAAAGATATTAGGGATATCCCAGGATTGGCACCTCCAGTTCCCACAAGGAGGCTTTTGTATGTTCGAACAGGGGAACTTTATGATTAATTCTTATATTCGAACCAGAGCTTTCTCCATCCTAGTCCTACCAAGTTGGTTCTTGTTCTTATCGATGGAAATATTAGAGGATCCTTTTCTGGAGGCATCGGTTTGATTTTTCTGGTTATTCTTTCTTTTTAAGGATTCCTGCATATACTGTTTGTGATTCTATGTAAATGAGGATCTTATGCTTTTCCTTGAGTGCCACGTCCACGTTTTTTTCTTGTAACATTCCAAGATTTAGGCCTAGAAAGAGTAATACTCTACTGTGAAATTCATGGTGTATATGTATATTTTATTTGGAGGTACCATTACCCCCTCCCCCTTTCCCCGATTTTTTGGTGTAATATATGCTTGCATTTTCAAACTGTCTGCTGGATCAGCCTTGTAAATCACATTTATTCCAAGTTATGTTTGCAGCTTAAGTTTTAAAAAATTT
TARGET=21,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04815 Ps_contig_mira-and-tgicl-ass_franssen_34711
SEQUENCE=CGCAGAGTACGCGGGGGTTGTCTCTCTCTCTTCCCTCACACATCGCACAACACAGGCTCAACTTCTCCGTTCCGTTGTGTTGTGTCGTGCTGTATTGTGCTGTGTCTTCTTTCTTCTTCTTCATTCTCTCACTATCACGCTAACGACCCTATTCTCAATTCCATCCAACAAACTCTGCATTTTCCATTTCTTTTTTTCCCTAAATCAATTACACTAATGCTTCTTCGTCTTCCTCTCTCATCAACAACCTAGGGTTTCTCTCCGATTTTCCTTTCATTTCGCCCGGATTTTGGGGTTAAGCTCAATCTCCGGCTTCCATGGCTTCCAACTTGAACATGACTCCTAAGCTCGAACAGATCCACGGTGAAATTCGCGATATCTTCCGAGCCCTTGCAAATGGCTTCCAGAAGCTGGATAAGATTAAAGATTCCAATAGACAAAGCAATCAGCTGGAGGAACTCACTGGGAAGATGAGGGATTGCAAAAGGTTGATAAAAGATTTTGATCGTGAAGTTAAAGATGAAGGGGCAGGAAATCCCGAAGAAGTGAATAAGCAACTCAATGACGAAAAGCAATCAATGATCAAGGAGCTAAACTCATATGTGGCACTGAGAAAAACGTACATGAATACCATTGGTAATAAAAAACTTGAACTTTTTGACATGGGAGCTGGTGCAAGTGAACCCGCTGAAGGAAATGTTCAACTCGCATCAGAAATGTCAAATCAAGAACTTGTCAATGCTGGGATGAAGACAATGGACGAGACTGATCAGGCTATTGAGAGGTCTAAGCAGGTTGTACATCAAACAATTGAAGTTGGCACCCAAACTGCTTCTACCTTGAAGGGCCAAACTGATCAAATGGGTCGTATTGTTAATGAGCTGGATTCAATTCAGTTTTCAATTAAGAAAGCCTCCCAACTTGTTAAAGAGATCGGTAGACAGGTAGCTACAGACAAATGCATCATGCTGTTTCTGTTTCTTATCGTCTGTGGTGTAATAGCCATTATTGTCGTGAAGATTGTGAATCCCAACAACAAAGATATTAGGGATATCCCAGGATTGGCACCTCCAGTTCCCACAAGGAGGCTTTTGTATGTTCGAACAGGGGAACTTTATGATTAATTCTTATATTCGAACCAGAGCTTTCTCCATCCTAGTCCTACCAAGTTGGTTCTTGTTCTTATCGATGGAAATATTAGAGGATCCTTTTCTGGAGGCATCGGTTTGATTTTTCTGGTTATTCTTTCTTTTTAAGGATTCCTGCATATACTGTTTGTGATTCTATGTAAATGAGGATCTTATGCTTTTCCTTGAGTGCCACGTCCACGTTTTTTTCTTGTAACATTCCAAGATTTAGGCCTAGAAAGAGTAATACTCTACTGTGAAATTCATGGTGTATATGTATATTTTATTTGGAGGTACCATTACCCCCTCCCCCTTTCCCCGATTTTTTGGTGTAATATATGCTTGCATTTTCAAACTGTCTGCTGGATCAGCCTTGTAAATCACATTTATTCCAAGTTATGTTTGCAGCTTAAGTTTTAAAAAATTT
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0953
PRIMER_LEFT_PENALTY=0.046801
PRIMER_RIGHT_PENALTY=0.048460
PRIMER_LEFT_SEQUENCE=CACAACACAGGCTCAACTTCTC
PRIMER_RIGHT_SEQUENCE=AAAGGAAAATCGGAGAGAAACC
PRIMER_LEFT=45,22
PRIMER_RIGHT=273,22
PRIMER_LEFT_TM=59.953
PRIMER_RIGHT_TM=59.952
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.1960
PRIMER_LEFT_1_PENALTY=0.046801
PRIMER_RIGHT_1_PENALTY=0.149184
PRIMER_LEFT_1_SEQUENCE=CACAACACAGGCTCAACTTCTC
PRIMER_RIGHT_1_SEQUENCE=TTGAGAATAGGGTCGTTAGCGT
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=157,22
PRIMER_LEFT_1_TM=59.953
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=0.3147
PRIMER_LEFT_2_PENALTY=0.046801
PRIMER_RIGHT_2_PENALTY=0.267873
PRIMER_LEFT_2_SEQUENCE=CACAACACAGGCTCAACTTCTC
PRIMER_RIGHT_2_SEQUENCE=GAGAGAGGAAGACGAAGAAGCA
PRIMER_LEFT_2=45,22
PRIMER_RIGHT_2=238,22
PRIMER_LEFT_2_TM=59.953
PRIMER_RIGHT_2_TM=60.268
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.3147
PRIMER_LEFT_3_PENALTY=0.046801
PRIMER_RIGHT_3_PENALTY=0.267873
PRIMER_LEFT_3_SEQUENCE=CACAACACAGGCTCAACTTCTC
PRIMER_RIGHT_3_SEQUENCE=TGAGAGAGGAAGACGAAGAAGC
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=239,22
PRIMER_LEFT_3_TM=59.953
PRIMER_RIGHT_3_TM=60.268
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=0.3938
PRIMER_LEFT_4_PENALTY=0.244611
PRIMER_RIGHT_4_PENALTY=0.149184
PRIMER_LEFT_4_SEQUENCE=GGGTTGTCTCTCTCTCTTCCCT
PRIMER_RIGHT_4_SEQUENCE=TTGAGAATAGGGTCGTTAGCGT
PRIMER_LEFT_4=14,22
PRIMER_RIGHT_4=157,22
PRIMER_LEFT_4_TM=60.245
PRIMER_RIGHT_4_TM=60.149
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_04816 Ps_contig_mira-and-tgicl-ass_franssen_34716
SEQUENCE=GCCCACTTGTATGTCANTTTNNNTTRATTGGTNAAANGAAANNATCATATGTGAATACTATATCTTTGTTCGTATCAACTTCTTGTGGCACAGTACTACCTTGCAACAAATTCTTGGTATCTTTATTGCATGTTGTTACCTGTGGATTCTTGTCATTCCACTGCTTGTATTCATGATTAATACTGTTTGGTGTAACCTCAAATCCAACAATACGRGCAGAACCAGTTTCAGGGTCCTTGTGATACATGACTCTAAAACTCAAGTGGTTATTGATAAAATATTTCTCCTCCTTGCTCCCTTGATAGTTTCCTTTGAACCCAACACGAAAACCATGTTCATATGTTGTAGATTGGATTCCATCCCTCCTTTGTCTAAGAACAGCAACTGGAAGGTTATCCAAAACCATGTTCACTCGATATTCATCATCAATTTTTTTCTTTAAAGCTCTTTGCAGATTCAGCATCCAAAGTTTGATGGCAAGTAACAGTACAGGACTGTTCATTCCTCATATGAAACGTATATATTGAGTTTTCAATACGATCACCTCGAAGAACCTCTCCCAAATTTTCTGCACTGTTCACAATCTTGGAAGGTTTGCAATACTTCAAGTAATAGTAGTCATAAGGAAGCTGAGTCTTTGTGGACGACAATTTGTTCACTTTGACATAGAGATCGTCCCCGGTTTGGAAATCGCGAGGAGCAACGCCGGGAAGGTAGAAGGAATGCACGGAGGAGAGGAGGATGAGGAAAGTGGTGAAGAATACGGTGGTTGAATGGACGGATACGGTGGTGTATCCCATCTTCTCCTTCATCTGAGGTGAGAGAGAAAAAAAGAGAGAGAGACAAAAAATGCAAACAAAAAAATGGAAAGTGAAATATGAAGAAAATCCCCGCGTACTCTGCGTTAG
TARGET=833,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04817 Ps_contig_mira-and-tgicl-ass_franssen_34723
SEQUENCE=AAACCATGAAAGAAAAGAATAGAAAATTAGAAGTTCCTCCACCACTAAACAAAACCAATCCATTCCATTCAACTTCACAATCCACCCAATCCAACCCAAAGAAAAAGAAACCAGCACTCTCCATAAATCAATGGCTACAACCAAGCTTCTATTATCATTCCCTCTCTTCCAACTGCAACTCCTCCCCACCACCACCACCACCGCCGCCGCCGCTGCCAAAATCAATCCTTCATTCAGTTTCCAGCACCGTAATGGATTCTCCCCACTCCGCTCCACGCCTAACGATTTCTTGGGGGATTTTGGAGCCAGAGACCCTTTCCCAGCCGAGGTTGAAAGTAAATTCGGAGATAAAGTTCTAGGTAGTTACAACACGGAACACAAAATCCTCATCCCCAATATCTCGGCTCTTTCTCTTTCTCAACAGGATTGTCTTCCCATTTCACCTCTACAGTCTCCTCTTTCCATAGACGATGCTAACCAGCTTATAAGAAAGGTTTTGGGTTGGAGACTTGTGAATGAGGAAGGAGTACTTAAACTCAGATGTCTGTGGAAATTGAGAGATTTTAAATGCGGAGTCGAACTCATTAACAGAATCTCCAAGGTCGTAGAGAATACAGGCCATTTCCCGAATATACACATAGAACAACCAAATCAAGTCAGAGCAGAGCTATCGACAACATCCATTGGTGGATTGAGTATGAATGATTTCATTGTAGCTGCAAAGATAGATGCAATAAAGACATCAGATCTTGTCCCAAGGAAAAGAGTTTGGGCTTAATGCTCCATGAGTTATCACTATTTTGTGTTATTTGATGAAATCTATTATTCTTTGCTTTCTTTTTTAAGTCTTTATACATGTTAGATGCTTTTTCATTTTTGTGTTTGGTAAGTGTAATTTTGAGTTTCAGGGTTCAAGTGTTCAACTAAGAATTAAACATTAGTTCCTCCCTTTCAAATTCTATAAGATGTTTTATAGAGATAATTGGTCCTAAATTATAAATTATAAGTCATTTTATCATTCCT
TARGET=186,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1831
PRIMER_LEFT_PENALTY=0.052729
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=CTCTCTTCCAACTGCAACTCCT
PRIMER_RIGHT_SEQUENCE=GTTGAGAAAGAGAAAGAGCCGA
PRIMER_LEFT=161,22
PRIMER_RIGHT=421,22
PRIMER_LEFT_TM=60.053
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.2737
PRIMER_LEFT_1_PENALTY=0.052729
PRIMER_RIGHT_1_PENALTY=0.220992
PRIMER_LEFT_1_SEQUENCE=CTCTCTTCCAACTGCAACTCCT
PRIMER_RIGHT_1_SEQUENCE=AAATGGGAAGACAATCCTGTTG
PRIMER_LEFT_1=161,22
PRIMER_RIGHT_1=439,22
PRIMER_LEFT_1_TM=60.053
PRIMER_RIGHT_1_TM=60.221
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.2841
PRIMER_LEFT_2_PENALTY=0.052729
PRIMER_RIGHT_2_PENALTY=0.231323
PRIMER_LEFT_2_SEQUENCE=CTCTCTTCCAACTGCAACTCCT
PRIMER_RIGHT_2_SEQUENCE=GGATTTTGTGTTCCGTGTTGTA
PRIMER_LEFT_2=161,22
PRIMER_RIGHT_2=385,22
PRIMER_LEFT_2_TM=60.053
PRIMER_RIGHT_2_TM=59.769
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=0.3072
PRIMER_LEFT_3_PENALTY=0.052729
PRIMER_RIGHT_3_PENALTY=0.254467
PRIMER_LEFT_3_SEQUENCE=CTCTCTTCCAACTGCAACTCCT
PRIMER_RIGHT_3_SEQUENCE=TTGAGAAAGAGAAAGAGCCGAG
PRIMER_LEFT_3=161,22
PRIMER_RIGHT_3=420,22
PRIMER_LEFT_3_TM=60.053
PRIMER_RIGHT_3_TM=60.254
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.3561
PRIMER_LEFT_4_PENALTY=0.254467
PRIMER_RIGHT_4_PENALTY=0.101680
PRIMER_LEFT_4_SEQUENCE=TCCTCCACCACTAAACAAAACC
PRIMER_RIGHT_4_SEQUENCE=ATTACGGTGCTGGAAACTGAAT
PRIMER_LEFT_4=34,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=60.254
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_04818 Ps_contig_mira-and-tgicl-ass_franssen_34723
SEQUENCE=AAACCATGAAAGAAAAGAATAGAAAATTAGAAGTTCCTCCACCACTAAACAAAACCAATCCATTCCATTCAACTTCACAATCCACCCAATCCAACCCAAAGAAAAAGAAACCAGCACTCTCCATAAATCAATGGCTACAACCAAGCTTCTATTATCATTCCCTCTCTTCCAACTGCAACTCCTCCCCACCACCACCACCACCGCCGCCGCCGCTGCCAAAATCAATCCTTCATTCAGTTTCCAGCACCGTAATGGATTCTCCCCACTCCGCTCCACGCCTAACGATTTCTTGGGGGATTTTGGAGCCAGAGACCCTTTCCCAGCCGAGGTTGAAAGTAAATTCGGAGATAAAGTTCTAGGTAGTTACAACACGGAACACAAAATCCTCATCCCCAATATCTCGGCTCTTTCTCTTTCTCAACAGGATTGTCTTCCCATTTCACCTCTACAGTCTCCTCTTTCCATAGACGATGCTAACCAGCTTATAAGAAAGGTTTTGGGTTGGAGACTTGTGAATGAGGAAGGAGTACTTAAACTCAGATGTCTGTGGAAATTGAGAGATTTTAAATGCGGAGTCGAACTCATTAACAGAATCTCCAAGGTCGTAGAGAATACAGGCCATTTCCCGAATATACACATAGAACAACCAAATCAAGTCAGAGCAGAGCTATCGACAACATCCATTGGTGGATTGAGTATGAATGATTTCATTGTAGCTGCAAAGATAGATGCAATAAAGACATCAGATCTTGTCCCAAGGAAAAGAGTTTGGGCTTAATGCTCCATGAGTTATCACTATTTTGTGTTATTTGATGAAATCTATTATTCTTTGCTTTCTTTTTTAAGTCTTTATACATGTTAGATGCTTTTTCATTTTTGTGTTTGGTAAGTGTAATTTTGAGTTTCAGGGTTCAAGTGTTCAACTAAGAATTAAACATTAGTTCCTCCCTTTCAAATTCTATAAGATGTTTTATAGAGATAATTGGTCCTAAATTATAAATTATAAGTCATTTTATCATTCCT
TARGET=201,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1831
PRIMER_LEFT_PENALTY=0.052729
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=CTCTCTTCCAACTGCAACTCCT
PRIMER_RIGHT_SEQUENCE=GTTGAGAAAGAGAAAGAGCCGA
PRIMER_LEFT=161,22
PRIMER_RIGHT=421,22
PRIMER_LEFT_TM=60.053
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.2737
PRIMER_LEFT_1_PENALTY=0.052729
PRIMER_RIGHT_1_PENALTY=0.220992
PRIMER_LEFT_1_SEQUENCE=CTCTCTTCCAACTGCAACTCCT
PRIMER_RIGHT_1_SEQUENCE=AAATGGGAAGACAATCCTGTTG
PRIMER_LEFT_1=161,22
PRIMER_RIGHT_1=439,22
PRIMER_LEFT_1_TM=60.053
PRIMER_RIGHT_1_TM=60.221
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.2841
PRIMER_LEFT_2_PENALTY=0.052729
PRIMER_RIGHT_2_PENALTY=0.231323
PRIMER_LEFT_2_SEQUENCE=CTCTCTTCCAACTGCAACTCCT
PRIMER_RIGHT_2_SEQUENCE=GGATTTTGTGTTCCGTGTTGTA
PRIMER_LEFT_2=161,22
PRIMER_RIGHT_2=385,22
PRIMER_LEFT_2_TM=60.053
PRIMER_RIGHT_2_TM=59.769
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=0.3072
PRIMER_LEFT_3_PENALTY=0.052729
PRIMER_RIGHT_3_PENALTY=0.254467
PRIMER_LEFT_3_SEQUENCE=CTCTCTTCCAACTGCAACTCCT
PRIMER_RIGHT_3_SEQUENCE=TTGAGAAAGAGAAAGAGCCGAG
PRIMER_LEFT_3=161,22
PRIMER_RIGHT_3=420,22
PRIMER_LEFT_3_TM=60.053
PRIMER_RIGHT_3_TM=60.254
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.3561
PRIMER_LEFT_4_PENALTY=0.254467
PRIMER_RIGHT_4_PENALTY=0.101680
PRIMER_LEFT_4_SEQUENCE=TCCTCCACCACTAAACAAAACC
PRIMER_RIGHT_4_SEQUENCE=ATTACGGTGCTGGAAACTGAAT
PRIMER_LEFT_4=34,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=60.254
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_04819 Ps_contig_mira-and-tgicl-ass_franssen_34724
SEQUENCE=ATAAAAAAAAAGRATGAAAATATGGCGANRGGRGAGAGCTTTTCTCGCCATTCATCATCTATGGCTCTTCTTCCATCTTCTTCTTCTTTCCACAAAACTATCTACCATATCTCATCCAAACTCTGTCATCCATGCCTTCATCTTCCTCAATTCAACCCCATCACTCTTGCTTCACCCTCTTTCAATCCACTGACTCATTCAAAGCAGCACCTAAACCTCAAAACCAGCAACTCCAACCTCTTTGTTGTTTCATACAGGTATTTCGCTAAAGAAGAAGATGAAGATGAAGAAGAGCACAGCTTTGATGAAGCAGTGACTCTATTTAATGGTGGAGAGTACTACAAATGTCATGACTACCTTGAATCCCTCTGGCACAATGCTGAAGAGCCATCAAGAACATTAATTCATGGCATATTGCAATGTGCAGTTGGGTTTCATCACCTCTTCAATCAGAATCATAAAGGAGCTATGATGGAGTTGGGAGAGGGATTATGTAAACTGCGAAAGATGGAATTCTCTAACGGACCGTTTCTTATGTTCGAGAAAGATATTTCAGCAGTTCTGGATTTTATCTACAAGACACAGATAGAGTTAGCTGCATGTAGTGATGATATCTGTGTTGCAATGGATCAATCAGAAAGATCCTATCAACTTATGGGTGAATATGCGGCAGGGAAGCGCGTATATGATCTAGAACTCGGCCGTGATGCATCGGTGTATATTGTATTATGCCGTCGAGGGTCTAATGGTGCCACTGAAGCCCCAAGGGTAAAGCTTCCCAGGCTTAATGCTACCTCAGAACATCTTGTAGACTATGAATACAGATAAATACTAAATTTATTATTCACTTCTACTAGAGACTACACGATGTATAAAATTGAAGAATAATGTTCTTTATAAATGGCATC
TARGET=76,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04820 Ps_contig_mira-and-tgicl-ass_franssen_34726
SEQUENCE=ACTTCCAAGAAGTTGTTCCTTTCCATTTCCTTCCAAGCTCTCATCATCCTTATCCTTTTCAAGTTGTTCTTTCAAAGTGAACTGAGGACCTAAATCAATCTTCCTCTCTACGTCGTCGTCGTCCTCTTCGGTGGCAGCAATAGAACTTTCACTCATGTGTCTACTAATGTCTTTCCTTGGCTCATTGCCACTTCCTTCATGATCACTGGATATGTCACGATCAGAATCCATGTTTTTGCAATTGGAGTTTGAAGATCACTCCAATAGTCAAAGACATCAAAGGTGG
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3188
PRIMER_LEFT_PENALTY=0.207756
PRIMER_RIGHT_PENALTY=0.111077
PRIMER_LEFT_SEQUENCE=TTTCCTTCCAAGCTCTCATCAT
PRIMER_RIGHT_SEQUENCE=TCCAGTGATCATGAAGGAAGTG
PRIMER_LEFT=25,22
PRIMER_RIGHT=209,22
PRIMER_LEFT_TM=60.208
PRIMER_RIGHT_TM=60.111
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=0.4403
PRIMER_LEFT_1_PENALTY=0.151533
PRIMER_RIGHT_1_PENALTY=0.288804
PRIMER_LEFT_1_SEQUENCE=CTTCCAAGCTCTCATCATCCTT
PRIMER_RIGHT_1_SEQUENCE=TGGAGTGATCTTCAAACTCCAA
PRIMER_LEFT_1=29,22
PRIMER_RIGHT_1=262,22
PRIMER_LEFT_1_TM=59.848
PRIMER_RIGHT_1_TM=59.711
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.4966
PRIMER_LEFT_2_PENALTY=0.207756
PRIMER_RIGHT_2_PENALTY=0.288804
PRIMER_LEFT_2_SEQUENCE=CATTTCCTTCCAAGCTCTCATC
PRIMER_RIGHT_2_SEQUENCE=TGGAGTGATCTTCAAACTCCAA
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=60.208
PRIMER_RIGHT_2_TM=59.711
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=0.4966
PRIMER_LEFT_3_PENALTY=0.207756
PRIMER_RIGHT_3_PENALTY=0.288804
PRIMER_LEFT_3_SEQUENCE=ATTTCCTTCCAAGCTCTCATCA
PRIMER_RIGHT_3_SEQUENCE=TGGAGTGATCTTCAAACTCCAA
PRIMER_LEFT_3=24,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=60.208
PRIMER_RIGHT_3_TM=59.711
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=0.4966
PRIMER_LEFT_4_PENALTY=0.207756
PRIMER_RIGHT_4_PENALTY=0.288804
PRIMER_LEFT_4_SEQUENCE=TTTCCTTCCAAGCTCTCATCAT
PRIMER_RIGHT_4_SEQUENCE=TGGAGTGATCTTCAAACTCCAA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=262,22
PRIMER_LEFT_4_TM=60.208
PRIMER_RIGHT_4_TM=59.711
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.5000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_04821 Ps_contig_mira-and-tgicl-ass_franssen_34727
SEQUENCE=CTCAAACAATCAGAGACTAAAGAAGAAAGGACAAGAAGGTAATGCATTAGGTAGGATAATACTAATGAGTTTGTATGAYGCATTTAACTTTGCATCTAATTCTAATTGGTATTCAAACACTGATTTGTCTACTATGGTTTCTGGTCTCTCTCAAGTCATTGGAACTTCTAATAATAATATTAATCCACACATGGCACAACCAACTTCAAGTACTATTCTCAATAAAGAATCTCAACCTCTTGTAGATCAAGAAGCAAAAAAGAAACCTCACTATAGAGGAGTGAGACAAAGACCATGGGGAAAATGGGCAGCAGAAATTCGTGATCCAATAAAAGCAGCTAGGGTTTGGCTAGGAACATTTGATACAGCTGAAGATGCAGCTCTTGCTTATGACAAAGCAGCACTTAAATTCAAAGGAACAAAAGCTAAACTTAATTTCCCCGAAAGAGTTGTTCAATGCAACAACAACAATAATTCTAGTAGTGGTTCTGTTGTTGATCAACAAGTTTTTCCGAATCTGTTTCAGTATGCTCAGATTCTCTCTAGTGATGCTGAGTTTCCTTATTATGCATCTCATCTTTTCAATCAAGATCAACAACAAGGAGATGGTGTTCATCATCCACCTGGTCAGTAGAGTCTACGCCGTCGGCGGATATCGGTGGTTATTCGATCAAGATCAATGGTCTATGTCAATTTTGCTCTTGAAACTAGAGTTGTGGATTTATGTCTTGTAAAAGGGGTTATTGAATATCCAAAATAGTTTCAAATTTGAACTTATGAGTGTATTGCAATTTAGCTACATTTTTCACGTTGTCAAACGTGCATTAAAGGTAAGATGGAACCAATTTCTGTTTACACTTATGTATGAGAAACAACTCTCAACTTGTTTGGATTTACTTTTGTAACTCAAAAGAAATTTATATTCTTAATAATAATGTATTTGGGTTGAGAATAATTCCACCTTATAAAACTTCTT
TARGET=460,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04822 Ps_contig_mira-and-tgicl-ass_franssen_34734
SEQUENCE=TCGGGGTAACCGTAATTAAACGTTTAAAGTCGTAAGGAGTTCAATTCAATTCAAAGCAACTATGGCGTCGAAAGATTCAACACCACCGTATCAGAGTGCTGCCAGAATCTCCGATTCCCAATGTTTTCCTCAATACACGGCTTCTCTCAAATGTTTAGAAGAGTTCAATACAGATAAGAGTAAATGCCAAGAACATTTTGATGTTTACAAGGAGTGTAAGAAAAAGGAGAGGGAAGCAAGATTGGAACGTAATAAAAGTCGGTCGCTATTCTCATGAAATCTTGGCAGAGTTTCACTAATTGTATCATAGGTAACAAAGTGAAGAGTTCACATGTATTACTGGTTCATTAGGGTTATGAAATCATCTCAAGCTATGACATTGCGGTATTCTATGCTGTCGTTCGTTTTGCATCTGTACTTAATTTAGGTTCCAATAAATTAACTGTTTACACTTTACTGCAGTATGTTGTTCCACTGAAACCTTGGTGGACCTTTGGATAACTAAAANRAYYGTGTTAGAAACTAAAAAAAAAGGT
TARGET=40,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04823 Ps_contig_mira-and-tgicl-ass_franssen_34740
SEQUENCE=AGTTGATCGCTTCCTTGATTCTCACCCAAATTACGATGGTCGTGGTGCTCTCATCGCTATTTTCGATTCTGGCATAGACCCGGCTGTTGATGGATTACAGGTTACATCAGATGGGAAACCAAAAATTCTTGATGTCATTGATTGTACTGGGAGTGGTGATATTGATACCTCAAAGGTCGTGAAGGCTGATGCATATGGTTGTATTTGGCGGGGCATCAGGGGCATCTTTGGTTATCAACACTTCATGGAAAATTCCTTCTGGTGAATGGCACGTAGGTTATAAATTGGTTTACGAGCTCTTTACAGAAGATTTGACCTCTCGTTTGAAGAAAGAAAGAAAGAAAAAATGGGATGAGAAAAACCAGGAGGAA
TARGET=327,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1751
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=1.172839
PRIMER_LEFT_SEQUENCE=GATACCTCAAAGGTCGTGAAGG
PRIMER_RIGHT_SEQUENCE=CCTGGTTTTTCTCATCCCATT
PRIMER_LEFT=163,22
PRIMER_RIGHT=365,21
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.173
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=1.2340
PRIMER_LEFT_1_PENALTY=0.061184
PRIMER_RIGHT_1_PENALTY=1.172839
PRIMER_LEFT_1_SEQUENCE=CTTCATGGAAAATTCCTTCTGG
PRIMER_RIGHT_1_SEQUENCE=CCTGGTTTTTCTCATCCCATT
PRIMER_LEFT_1=240,22
PRIMER_RIGHT_1=365,21
PRIMER_LEFT_1_TM=59.939
PRIMER_RIGHT_1_TM=60.173
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=1.3793
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=1.377001
PRIMER_LEFT_2_SEQUENCE=GATACCTCAAAGGTCGTGAAGG
PRIMER_RIGHT_2_SEQUENCE=CCTGGTTTTTCTCATCCCATTT
PRIMER_LEFT_2=163,22
PRIMER_RIGHT_2=365,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=61.377
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=1.4219
PRIMER_LEFT_3_PENALTY=0.249074
PRIMER_RIGHT_3_PENALTY=1.172839
PRIMER_LEFT_3_SEQUENCE=GGCTGTTGATGGATTACAGGTT
PRIMER_RIGHT_3_SEQUENCE=CCTGGTTTTTCTCATCCCATT
PRIMER_LEFT_3=81,22
PRIMER_RIGHT_3=365,21
PRIMER_LEFT_3_TM=60.249
PRIMER_RIGHT_3_TM=60.173
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=1.4305
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=1.428205
PRIMER_LEFT_4_SEQUENCE=GATACCTCAAAGGTCGTGAAGG
PRIMER_RIGHT_4_SEQUENCE=TCCTCCTGGTTTTTCTCATCC
PRIMER_LEFT_4=163,22
PRIMER_RIGHT_4=369,21
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=60.428
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_04824 Ps_contig_mira-and-tgicl-ass_franssen_34741
SEQUENCE=GAGCTCAAATAAGATCATCTCACTTTGGTTCATAGCCACATAATTCATTATTATTGTACAAACAAGTAGTATCTTCACAATAAAATAGCAACATAAATTCCACCAATTTTCGATTTCTTTCTCTCCTATGTTTACTATTCAATCAAGGAAAAATCATAAGAGTGCAGAAGAATATGCAGAGCAACTCCACTGTAAGAATAAGTAGAGTGATAAGTGAGAATCTGGCGTTGGGAAGGCCAATACGAATTTTAACTGATGTATGTCGTTGCTCCAAGGACTTTAGATGGAATATTTTACGCCAGTTTGTCCGGTATTAGTAATTACTTCGAATTCTCCTGCAGTAAATGACCTGTCTTTCTTGAAAATCGGGCGTCTTAGTTCGACATATCCACAATAGTCAACTTCATATACTTCAGCACCCTTTCTGAATGAAGATAGAGCCACACCAACTCCTTCCCCATGTGTGACAAGAAGCAAGTTTTCAGTAAGATGTTTATCAGCAAGATCTTTAACTATTTGCTCATATCTAGCCTTGGTGCTTGCAATCGGCTCCTCATACTTAGGCAACTCTTTATAGACCCTTTCTACATTTTTATCGACCGTCCCTGCCGGAAGCATGGCCTCGCGCTCTGATATGTTGAAACCCCAGTTTCCATCTTTAGGAGCCACATCAAGCCTAATCGCCATTCTGCTCATCATTTCACATAATCCATATTCAACAGAGGCCTTAATTTTAGAAGGATCGAAGCTAACAGACTCTCCGGTGAGTGCTTCGGGGCTATCATCGACGGCGGAGAGAGCGATAACGGCTTCCGCGGCGGTCTGAACGCAGCGGAGGAACGGTGAAACGAAGACACGATGAAGCGGATATTTGAAAAGATTGCGAAACTTCCGACCCGTACAGAAGGACCGAACCCGACCCTCTTGAACGAGAGGTGGGTCCCACGGTCGTTGCGCTGTGAGTGTCCATAGCGGATCGAAGTTGTCGATGCGATCGCCGTGTCTCATTACGACGACGTTTTGGTAATATTCTGGAGTTTCGTTTTTCGATGTTGAATCCATTTTCTTTTCTTTTCTGAATTTCAGATTTTCAGAATCNNCGAAAGAGAGAGAATC
TARGET=1062,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04825 Ps_contig_mira-and-tgicl-ass_franssen_34744
SEQUENCE=ACCTAAAAATGTGATTGTTGAGAACCCGAAAAAGTTGTTTTATTACTTTGTGCTGTAATCGAATGTACATGTGTCAATAAATAAACAGCCGTCAAATGACTGATATATATCTGAATTAGAACCAAGTAATAATATAACAAATGATGTATAACAGCAATGGAGACTGTTTTACGTAGCCACCTGCTGTAATGGAGGCCAGGCAATATCATCATCATCTGTGTGGCCCAGAAAAAGAAAATCAACACAGTTCTTCCACCAACCATGATTATATGGATTTCTGAACCGCCCATCAGGACCGCGCAGATAATCATAACGTAAGGAATTTGCTAGTTCGTTTGTGGTCACATTCCGTGCTATCATGGAAGCCTGTGTTACTGTCAAAGTTGTAGCTGCAACGACGACAACGGCGTCCATCACCAAAAATGCAACAAGACCTGGATTTCTTACTAACACCTGATGAATCCATGTTTCTCCTGCTGGTAATGCTGGTATTGATGTCCAAATTCTATGGACTGCAATGCCGGCACTGACGGAAGACGTTATAGTTCCCAAGCAGATTAAAATAAAGAAATCCCTTTTGTTCCTCTTCCCAACACAATTAGATATCCACGGACAATGGTGGTCGAACTGTTCCACGCAACGCTTACAAGTAGGGCAATGCTTAGATCGGACAGGTCTTATTATCTTGCAGGTAGGGCAAAGTTGAGACCAGTTTCCCATCCAAACAGAGGAACTGTTCAGCTCAATATTCAACAAGGGATCCTCTGAATCGCTTAGAGTACCCGAGTCTCCGGGCCGTTTTATATAACCTGGATCTCGTCTACTACACCTATAAAACATAACCAAGGAACCAACAGATGAAGAGAGAGCTACCCATGACCAAAATCCAACAACAGCTGTAATCTTTTTAAGGTTAGGAGCTGCAACAACTGAATTGATGAAGAGGATTGAAAGAGAGACTATAATACACAATAAAATGGGAGCATAACCAATATCTGTCACCATCCCACAATAAAGTTTCCCTCTCCAGTGGCTACTATGAGCCCGTTGTTGATTAGAAAGGAAAGGGGCGACATGTCGATGACCTTTATCATATGCAAGCTGTACAGGAGTGAATCCTGCATTGTCCTTCACCATCAATTCTTCCTTTGTTCCAGCATGCACAAGCACAGCGCACGCCTCTGAATGTCCTCTTAATGCTGCCCAATGCAATGGGGTACAACCATCTTTATCTTGTCTTCCTTGACTCGCATCTCTAAATAGAAGCAATCTTATTGTATCAGCATATCCCTTATACGCAGCCCAATGTAGAGGACTCCTTCCATCATTATCGGGCACATCAAAATCAGCATGGTATTTTACAACAATGTGATTTAAGAAAGCTGTCTGTCCATACTGTGCAGCAACATGAACCGGTATCCATTTATATCAGAAGCTTCAACCCGAGCACCATTCTCCAAGAGCACATCAGCAGCTGCAATTGAACCACGAACTGCCGCCCAGTGTAAAGCTGTTTGTTGCATATTGTCTTTAGCATTGACATCACCACCATGCTGAATGAGATAGTGAACAATGTCGTGAAAGTTATTGAGAGAAGCCCATTGAAGAGCATAATATCCATTAACATCAGGAACAGAAACGGAGGCACCGTCTTGTTCAACGAACGTTCTCAATTTCTGAAAGTCGCCGTGAGAAGAGGCAGTGAAGACGTCGAGTATGAGAGGTAGCTGGTGGTCTTGGGGCTTGGACTCCGTCGATACGACTTCGATCTCCGACGAAGCCATCGGAAAGGGACTCAGGAGGAAGATGCTAGTGCATTCGATAATTGAATAGGAAAAGGTAATTGATGGTGTACGTTTAGGTTTTAG
TARGET=205,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0706
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.050763
PRIMER_LEFT_SEQUENCE=GATTGTTGAGAACCCGAAAAAG
PRIMER_RIGHT_SEQUENCE=GTTGGTGGAAGAACTGTGTTGA
PRIMER_LEFT=12,22
PRIMER_RIGHT=259,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.051
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.1673
PRIMER_LEFT_1_PENALTY=0.162964
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=TTTTACGTAGCCACCTGCTGTA
PRIMER_RIGHT_1_SEQUENCE=AGAAATCCAGGTCTTGTTGCAT
PRIMER_LEFT_1=166,22
PRIMER_RIGHT_1=443,22
PRIMER_LEFT_1_TM=59.837
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.1880
PRIMER_LEFT_2_PENALTY=0.019805
PRIMER_RIGHT_2_PENALTY=0.168187
PRIMER_LEFT_2_SEQUENCE=GATTGTTGAGAACCCGAAAAAG
PRIMER_RIGHT_2_SEQUENCE=GGCGGTTCAGAAATCCATATAA
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=286,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=60.168
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=5.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.1977
PRIMER_LEFT_3_PENALTY=0.193337
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=CAGCAATGGAGACTGTTTTACG
PRIMER_RIGHT_3_SEQUENCE=AGAAATCCAGGTCTTGTTGCAT
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=443,22
PRIMER_LEFT_3_TM=59.807
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.1977
PRIMER_LEFT_4_PENALTY=0.193337
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=TGGAGACTGTTTTACGTAGCCA
PRIMER_RIGHT_4_SEQUENCE=AGAAATCCAGGTCTTGTTGCAT
PRIMER_LEFT_4=157,22
PRIMER_RIGHT_4=443,22
PRIMER_LEFT_4_TM=59.807
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_04826 Ps_contig_mira-and-tgicl-ass_franssen_34745
SEQUENCE=ATTGCTCGAAGAAACTCCCCGATGGTGTCTACCCTTGCGTACCTGGATGACACGCCTATGGCCAGTTCCATCCCAATAACTGTATGTGATTTGAAGAGGCTCGTTTCGAATTTGGTCCTGCTCACGGAGCCACTGTTTGCGCAGCCTTTCGCGTTCAGCTTGCTCCTCAGCCTCTCGCTCACTGTCAGGTAGAAAGCTAGTTTCGACGGTGGGGTCTTTACCAAGCTTACCACACCGTACTCCTCCATTTGTTTCTATATTATTACTTTGATGTGTCTCCTCCTCTTCCTGAGCATCATTGTCAATAATATCTTCGGAAAATGATAGTCTCGAATTTCCCTTGATCTTCCTCTTTTTGCGTTTTTGTAATTGAAGCTCCTCCTCCTCTTGTAGTTGCTTCTGAAGCTTCTCCTTCTCTTCCT
TARGET=376,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6254
PRIMER_LEFT_PENALTY=0.014792
PRIMER_RIGHT_PENALTY=1.610635
PRIMER_LEFT_SEQUENCE=ACCAAGCTTACCACACCGTACT
PRIMER_RIGHT_SEQUENCE=GGAAGAGAAGGAGAAGCTTCAGA
PRIMER_LEFT=219,22
PRIMER_RIGHT=420,23
PRIMER_LEFT_TM=59.985
PRIMER_RIGHT_TM=60.611
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.1000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=1.8854
PRIMER_LEFT_1_PENALTY=0.274768
PRIMER_RIGHT_1_PENALTY=1.610635
PRIMER_LEFT_1_SEQUENCE=CACCGTACTCCTCCATTTGTTT
PRIMER_RIGHT_1_SEQUENCE=GGAAGAGAAGGAGAAGCTTCAGA
PRIMER_LEFT_1=232,22
PRIMER_RIGHT_1=420,23
PRIMER_LEFT_1_TM=60.275
PRIMER_RIGHT_1_TM=60.611
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=1.9065
PRIMER_LEFT_2_PENALTY=0.295825
PRIMER_RIGHT_2_PENALTY=1.610635
PRIMER_LEFT_2_SEQUENCE=AGCTTACCACACCGTACTCCTC
PRIMER_RIGHT_2_SEQUENCE=GGAAGAGAAGGAGAAGCTTCAGA
PRIMER_LEFT_2=223,22
PRIMER_RIGHT_2=420,23
PRIMER_LEFT_2_TM=59.704
PRIMER_RIGHT_2_TM=60.611
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=1.9313
PRIMER_LEFT_3_PENALTY=0.320679
PRIMER_RIGHT_3_PENALTY=1.610635
PRIMER_LEFT_3_SEQUENCE=TCGCTCACTGTCAGGTAGAAAG
PRIMER_RIGHT_3_SEQUENCE=GGAAGAGAAGGAGAAGCTTCAGA
PRIMER_LEFT_3=174,22
PRIMER_RIGHT_3=420,23
PRIMER_LEFT_3_TM=59.679
PRIMER_RIGHT_3_TM=60.611
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=1.9313
PRIMER_LEFT_4_PENALTY=0.320679
PRIMER_RIGHT_4_PENALTY=1.610635
PRIMER_LEFT_4_SEQUENCE=CTCGCTCACTGTCAGGTAGAAA
PRIMER_RIGHT_4_SEQUENCE=GGAAGAGAAGGAGAAGCTTCAGA
PRIMER_LEFT_4=173,22
PRIMER_RIGHT_4=420,23
PRIMER_LEFT_4_TM=59.679
PRIMER_RIGHT_4_TM=60.611
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_04827 Ps_contig_mira-and-tgicl-ass_franssen_34746
SEQUENCE=GACCGTCCATAACACGCTCGCGGACATTTCTTCTCTGGCTCGCAACTCAGCGCTGTGCCTCTCGTCTTCTCCCTCGTCGCTCTTCAACTCAGACAGCAGGTTCCATAATTTTTTATCTGAAGAGAAAAAGAGAGAGAGATGTCGGGTATGGGAGATGGATACGTGGGCACTGCCCAAGATGCAGTTCGGATCCGACGGCTAGAGAAACAGAGAGAAGCTGAACGTCGTAAAATCCAAGAGCTGAAAACCAAATCTGCCACCTCCAATGGCCAACCCGGTCTCCTCCAATTTGGCTCCAGCACTTCTGAGATTCTTGAAACTGCTTTTAAGAAAGAAACTGTTGGTCTTGTCACCCGTGAACAGTATGTGGAGAAGAGGGTTAATATTCAAAGTAAAATAGAGGAGGAAGAGAAGAGAAGCTTCAGAAGCAACTACAAGAGGAGGAGGAGCTTCAATTAACAAA
TARGET=129,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9369
PRIMER_LEFT_PENALTY=0.863548
PRIMER_RIGHT_PENALTY=0.073355
PRIMER_LEFT_SEQUENCE=CTTCAACTCAGACAGCAGGTTC
PRIMER_RIGHT_SEQUENCE=TACTGTTCACGGGTGACAAGAC
PRIMER_LEFT=81,22
PRIMER_RIGHT=364,22
PRIMER_LEFT_TM=59.136
PRIMER_RIGHT_TM=60.073
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.9369
PRIMER_LEFT_1_PENALTY=0.863548
PRIMER_RIGHT_1_PENALTY=0.073355
PRIMER_LEFT_1_SEQUENCE=TCTTCAACTCAGACAGCAGGTT
PRIMER_RIGHT_1_SEQUENCE=TACTGTTCACGGGTGACAAGAC
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=364,22
PRIMER_LEFT_1_TM=59.136
PRIMER_RIGHT_1_TM=60.073
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.9652
PRIMER_LEFT_2_PENALTY=0.863548
PRIMER_RIGHT_2_PENALTY=0.101680
PRIMER_LEFT_2_SEQUENCE=CTTCAACTCAGACAGCAGGTTC
PRIMER_RIGHT_2_SEQUENCE=GCAGATTTGGTTTTCAGCTCTT
PRIMER_LEFT_2=81,22
PRIMER_RIGHT_2=256,22
PRIMER_LEFT_2_TM=59.136
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=176
PRIMER_PAIR_PENALTY_3=0.9652
PRIMER_LEFT_3_PENALTY=0.863548
PRIMER_RIGHT_3_PENALTY=0.101680
PRIMER_LEFT_3_SEQUENCE=TCTTCAACTCAGACAGCAGGTT
PRIMER_RIGHT_3_SEQUENCE=GCAGATTTGGTTTTCAGCTCTT
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=256,22
PRIMER_LEFT_3_TM=59.136
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=0.9995
PRIMER_LEFT_4_PENALTY=0.863548
PRIMER_RIGHT_4_PENALTY=0.135999
PRIMER_LEFT_4_SEQUENCE=CTTCAACTCAGACAGCAGGTTC
PRIMER_RIGHT_4_SEQUENCE=GGATTTTACGACGTTCAGCTTC
PRIMER_LEFT_4=81,22
PRIMER_RIGHT_4=234,22
PRIMER_LEFT_4_TM=59.136
PRIMER_RIGHT_4_TM=60.136
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_04828 Ps_contig_mira-and-tgicl-ass_franssen_34746
SEQUENCE=GACCGTCCATAACACGCTCGCGGACATTTCTTCTCTGGCTCGCAACTCAGCGCTGTGCCTCTCGTCTTCTCCCTCGTCGCTCTTCAACTCAGACAGCAGGTTCCATAATTTTTTATCTGAAGAGAAAAAGAGAGAGAGATGTCGGGTATGGGAGATGGATACGTGGGCACTGCCCAAGATGCAGTTCGGATCCGACGGCTAGAGAAACAGAGAGAAGCTGAACGTCGTAAAATCCAAGAGCTGAAAACCAAATCTGCCACCTCCAATGGCCAACCCGGTCTCCTCCAATTTGGCTCCAGCACTTCTGAGATTCTTGAAACTGCTTTTAAGAAAGAAACTGTTGGTCTTGTCACCCGTGAACAGTATGTGGAGAAGAGGGTTAATATTCAAAGTAAAATAGAGGAGGAAGAGAAGAGAAGCTTCAGAAGCAACTACAAGAGGAGGAGGAGCTTCAATTAACAAA
TARGET=438,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04829 Ps_contig_mira-and-tgicl-ass_franssen_34747
SEQUENCE=AGTGGTATCAACGCAGAGTGAGTGGTCTTATAGGACCTGCAAGAACGCAGAGATTAGTGAATGGAGCATTAGTACTAATCACTCTGGGAGGAAATGATTTTGTGAATTAATTTATTTATTTGGTGCCTTATTCTGCAAGATCGCGCCAATCTCTTACGGNTTATGTCAGATATATCATTATCTAGAATACAAAGAAGTATTTACGTAAAGGGTAGGTTACATGATTCGTTGGGAG
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5178
PRIMER_LEFT_PENALTY=0.276218
PRIMER_RIGHT_PENALTY=0.241544
PRIMER_LEFT_SEQUENCE=CTTATAGGACCTGCAAGAACGC
PRIMER_RIGHT_SEQUENCE=CTCCCAACGAATCATGTAACCT
PRIMER_LEFT=26,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=60.242
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.5624
PRIMER_LEFT_1_PENALTY=0.320870
PRIMER_RIGHT_1_PENALTY=0.241544
PRIMER_LEFT_1_SEQUENCE=TATCAACGCAGAGTGAGTGGTC
PRIMER_RIGHT_1_SEQUENCE=CTCCCAACGAATCATGTAACCT
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=234,22
PRIMER_LEFT_1_TM=60.321
PRIMER_RIGHT_1_TM=60.242
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.7281
PRIMER_LEFT_2_PENALTY=0.486593
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=CAACGCAGAGTGAGTGGTCTTA
PRIMER_RIGHT_2_SEQUENCE=CTCCCAACGAATCATGTAACCT
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=234,22
PRIMER_LEFT_2_TM=60.487
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=0.7610
PRIMER_LEFT_3_PENALTY=0.519421
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=GCAGAGATTAGTGAATGGAGCA
PRIMER_RIGHT_3_SEQUENCE=CTCCCAACGAATCATGTAACCT
PRIMER_LEFT_3=46,22
PRIMER_RIGHT_3=234,22
PRIMER_LEFT_3_TM=59.481
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.7816
PRIMER_LEFT_4_PENALTY=0.540037
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=ACTCTGGGAGGAAATGATTTTG
PRIMER_RIGHT_4_SEQUENCE=CTCCCAACGAATCATGTAACCT
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=234,22
PRIMER_LEFT_4_TM=59.460
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_04830 Ps_contig_mira-and-tgicl-ass_franssen_34750
SEQUENCE=TTCGGATTGGTAAGCGGTGTGAATGAACACACACTTCATTCTTTCTTCTCTTTCTCTCTCACACATATAACCCAACACAAATCTCATTTTTCTCTATAGTCTCTTCCACAATTTCAATCCACTCCATTTTTCATGGCTTCTCTCAATCTCCTTCATCACCCTTCAACTTCTATATCATCTTCTTCTTGTTGTTGTTGTTTATTTCCCAGGAGTTTTATTCCCCGCAACAACAATCTCACTCCAAAGGGTGGTAGTAACCATATCAAACCATCGCTTATTGTTAAATCTGTTGCAGGTGACCCCTTTACCGATGGAACTATTCCAATTCCTTCTAATCCATTTGACGCTATCCCCTCCTTAAAGCTCAATCTTCTGAGTGCTGTTTCTGGGCTAAATAGAGGTCTTGCGGCAAATGAAGAAGACCTGCAAAAGGCAGATGCTGCGGCTAAGGAACTCGAAGATGCTGGAGGATTGGTAGACCTCACAGATAACCTTGATAAATTGCAAGGAAGATGGAAACTGATATATAGCAGCGCGTTCTCCTCTCGTACTCTAGGTGGGAGCCGGCCTGGACCTCCCATAGGAAGGCTCCTTCCCATAACTCTTGGACAGGTTTTTCAACGAATTGACATCTTAAGCAAAGACTTTGACAATATAGTGGAGCTTCAGTTTGGTGCTCCGTGGCCTCTTCCACCCCTCGAAGCAACCGCCACATTAGCTCACAAATTTGAACTCATAGGATCTTCGAAGATCAAGATAATATTCGAGAAAACCACGGTGAAAACAACTGGGAATTTTTCACAGTTGCCGCCGTTAGAGGTACCTCAACTTCCAGAGGCATTAAGGCCAAAATCTAATAGAGGAAGTGGTGATTTTGAAGTTACATATCTTGACGCAGATACCCGCGTCACGAGAGGAGATAGAGGCGAGCTAAGAGTCTTTGTGATTTCATAAGTGTATGAAGTATGGACTATGCTCTTTATGATATTTCAATATTGTAACATTGATATACAAACCCTCACTATGGAGCTGGAAATGGTACTTTTAGTTTAAGTGTAACATATTTAATCTTAAAGGAATATTTGTTTATAGATCATTACATATAGATGCTGATATACA
TARGET=186,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0885
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.028588
PRIMER_LEFT_SEQUENCE=AATCTCCTTCATCACCCTTCAA
PRIMER_RIGHT_SEQUENCE=ATAGTTCCATCGGTAAAGGGGT
PRIMER_LEFT=144,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=10.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.1025
PRIMER_LEFT_1_PENALTY=0.073866
PRIMER_RIGHT_1_PENALTY=0.028588
PRIMER_LEFT_1_SEQUENCE=TCTCAATCTCCTTCATCACCCT
PRIMER_RIGHT_1_SEQUENCE=ATAGTTCCATCGGTAAAGGGGT
PRIMER_LEFT_1=140,22
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.971
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=10.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.1050
PRIMER_LEFT_2_PENALTY=0.073866
PRIMER_RIGHT_2_PENALTY=0.031122
PRIMER_LEFT_2_SEQUENCE=TCTCAATCTCCTTCATCACCCT
PRIMER_RIGHT_2_SEQUENCE=ACTACCACCCTTTGGAGTGAGA
PRIMER_LEFT_2=140,22
PRIMER_RIGHT_2=254,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=60.031
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=115
PRIMER_PAIR_PENALTY_3=0.1387
PRIMER_LEFT_3_PENALTY=0.110131
PRIMER_RIGHT_3_PENALTY=0.028588
PRIMER_LEFT_3_SEQUENCE=TCTCCTTCATCACCCTTCAACT
PRIMER_RIGHT_3_SEQUENCE=ATAGTTCCATCGGTAAAGGGGT
PRIMER_LEFT_3=146,22
PRIMER_RIGHT_3=319,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.971
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=10.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.1413
PRIMER_LEFT_4_PENALTY=0.110131
PRIMER_RIGHT_4_PENALTY=0.031122
PRIMER_LEFT_4_SEQUENCE=TCTCCTTCATCACCCTTCAACT
PRIMER_RIGHT_4_SEQUENCE=ACTACCACCCTTTGGAGTGAGA
PRIMER_LEFT_4=146,22
PRIMER_RIGHT_4=254,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=60.031
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_04831 Ps_contig_mira-and-tgicl-ass_franssen_34753
SEQUENCE=CAGGTTGGTCGTGTTCCTAACGACTTCTTCCTTTCCTTCCTTCCTACTGCCAACCTAACCAACTAATTTACCTACGCATTTCTAATGGCATCATCCACTCTTCCCATTGCATCATAGGTGCCTGCAAGATTGCTATAGACTCCTAATGTATCTTGATGATAAGGGCCATACTCTTTTTCCAATATAGTTCTAGCCTCTTCAAACAAATCTGCAGCTTCATTTATAGCATACCTTTGGACACAAGCAAGACCCATTTGATTCAATGCAATTCCAAACAATGCAGATTTTTTCTCCCCGCTTGCGCGAAATTTCGTAACCGAACTTTTAAAAATGTTATAAGAATCAGAATAATTCCCTAACATGTAATACATAACTCCCATTTGTGCCTCAATTCCAGCAGTAGTACTTTGTTGACCAGGAACATTGTTATATATTTTCAATGTCTTTTTTGAGTAACTTCAATCCYTTTTTCCAAATCATTCATAGATTGATAAATTGCAGACAACATTCTATAACCAAACCTATTAG
TARGET=33,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04832 Ps_contig_mira-and-tgicl-ass_franssen_34754
SEQUENCE=TATATGCAATAACTAAAAAACTTGTACATATTCAACAAGAGACACACCATTTACTCTCTCTCTCTCTCTCTCCCTTAGTATAGACATGGCTCTTCTTCAACCTACACCTTGAAATCAACAAAATCTTGTGTTTGTTTGTCTCTATGTTTGTTAGACTCAAACACAGATAGTGATATCTATTTCATTGTCTTGTTTTTTGTTCTGTTTTTGCATGAAACAGAGTACTCTGTTTCATCTCCTTTGTGATGAGGTATATTAGGACAAATAGCTTTAAGAGACTCTTCTCATTCAAAAAACGTTCTTTAGAGGAAGAAAAAGATAACACCTTGAAGATTCTTCCATATGAAGAACAACATTTTCAAAAACCCACTTGGAAATGTTTCTCCTATGAAGAGTTGTTTGATGCCACCAATGGCTTCAACTCAGAAAACATTGTTGGAAAAGGAGGCTATGCAGAAGTTTACAAAGGAATATTGAAAAATGGTGAAGAAATTGCTGTGAAGAGATTAACAAGAACTTCAAGAGATGAGCGAAAAGAGAAAGAGTTTTTGACAGAGATTGGTACAATAGGACATGTTCGTCATACGAATGTCTTGTCTCTCCTAGGATGTTGTATTGACAATGGACTTTACTTTGTTTTTGAGTTATCCACAACAGGTTCTGTTTCATCTATTCTTCATGATGAAAAGTTGGCACCTTTAGATTGGAAAACTAGGTGTAAGATAGTTCTTGGGACGGCGCGTGGACTTCACTACTTGCATAAAGGTTGCAAGAGGAGAATAATTCATAGAGATATCAAAGCATCAAACATTTTATTGACCAAAGATTTTGAACCACAGATATCTGATTTTGGATTAGCAAAATGGCTTCCATCTCAATGGACTCATCATTCAATTGCTCCAATAGAAGGAACATTTGGACATTTGGCACCAGAATACTATTTGCATGGAGTGGTGGATGAGAAGACAGATGTGTTTGCATTTGGTGTTTTCTTGCTTGAAGTCATTTCTGGTAGGAAACCTGTTGATGGGTCTCACCAAAGCTTACATAGTTGGGCTAAACCAATTTTGAACAAGGAAGAGATAGAAGAGCTAGTAGATGCAAGGCTTGAAGGGAGTTATGATGTGAAACAATTAAAGAGATTTGCCTTTGCTGCCTCTCTTTGTATTAGGGCATCTTCCACTTGGAGACCTAACATGAGTGAGGTAATGGAAATAATAGAGGAGGGAGAGATAGATAAAGAGAAATGGAAAATGCCAGAGGAAGAAGAAGAACAAGAAGATGAATTCTGGGGGTTTGAGGATTTGGAA
TARGET=54,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04833 Ps_contig_mira-and-tgicl-ass_franssen_34754
SEQUENCE=TATATGCAATAACTAAAAAACTTGTACATATTCAACAAGAGACACACCATTTACTCTCTCTCTCTCTCTCTCCCTTAGTATAGACATGGCTCTTCTTCAACCTACACCTTGAAATCAACAAAATCTTGTGTTTGTTTGTCTCTATGTTTGTTAGACTCAAACACAGATAGTGATATCTATTTCATTGTCTTGTTTTTTGTTCTGTTTTTGCATGAAACAGAGTACTCTGTTTCATCTCCTTTGTGATGAGGTATATTAGGACAAATAGCTTTAAGAGACTCTTCTCATTCAAAAAACGTTCTTTAGAGGAAGAAAAAGATAACACCTTGAAGATTCTTCCATATGAAGAACAACATTTTCAAAAACCCACTTGGAAATGTTTCTCCTATGAAGAGTTGTTTGATGCCACCAATGGCTTCAACTCAGAAAACATTGTTGGAAAAGGAGGCTATGCAGAAGTTTACAAAGGAATATTGAAAAATGGTGAAGAAATTGCTGTGAAGAGATTAACAAGAACTTCAAGAGATGAGCGAAAAGAGAAAGAGTTTTTGACAGAGATTGGTACAATAGGACATGTTCGTCATACGAATGTCTTGTCTCTCCTAGGATGTTGTATTGACAATGGACTTTACTTTGTTTTTGAGTTATCCACAACAGGTTCTGTTTCATCTATTCTTCATGATGAAAAGTTGGCACCTTTAGATTGGAAAACTAGGTGTAAGATAGTTCTTGGGACGGCGCGTGGACTTCACTACTTGCATAAAGGTTGCAAGAGGAGAATAATTCATAGAGATATCAAAGCATCAAACATTTTATTGACCAAAGATTTTGAACCACAGATATCTGATTTTGGATTAGCAAAATGGCTTCCATCTCAATGGACTCATCATTCAATTGCTCCAATAGAAGGAACATTTGGACATTTGGCACCAGAATACTATTTGCATGGAGTGGTGGATGAGAAGACAGATGTGTTTGCATTTGGTGTTTTCTTGCTTGAAGTCATTTCTGGTAGGAAACCTGTTGATGGGTCTCACCAAAGCTTACATAGTTGGGCTAAACCAATTTTGAACAAGGAAGAGATAGAAGAGCTAGTAGATGCAAGGCTTGAAGGGAGTTATGATGTGAAACAATTAAAGAGATTTGCCTTTGCTGCCTCTCTTTGTATTAGGGCATCTTCCACTTGGAGACCTAACATGAGTGAGGTAATGGAAATAATAGAGGAGGGAGAGATAGATAAAGAGAAATGGAAAATGCCAGAGGAAGAAGAAGAACAAGAAGATGAATTCTGGGGGTTTGAGGATTTGGAA
TARGET=1263,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1870
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.182645
PRIMER_LEFT_SEQUENCE=GATGGGTCTCACCAAAGCTTAC
PRIMER_RIGHT_SEQUENCE=AACCCCCAGAATTCATCTTCTT
PRIMER_LEFT=1025,22
PRIMER_RIGHT=1296,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.183
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.1870
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.182645
PRIMER_LEFT_1_SEQUENCE=GATGGGTCTCACCAAAGCTTAC
PRIMER_RIGHT_1_SEQUENCE=AAACCCCCAGAATTCATCTTCT
PRIMER_LEFT_1=1025,22
PRIMER_RIGHT_1=1297,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=60.183
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.2697
PRIMER_LEFT_2_PENALTY=0.087031
PRIMER_RIGHT_2_PENALTY=0.182645
PRIMER_LEFT_2_SEQUENCE=CCTTTGCTGCCTCTCTTTGTAT
PRIMER_RIGHT_2_SEQUENCE=AACCCCCAGAATTCATCTTCTT
PRIMER_LEFT_2=1146,22
PRIMER_RIGHT_2=1296,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=60.183
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=151
PRIMER_PAIR_PENALTY_3=0.2697
PRIMER_LEFT_3_PENALTY=0.087031
PRIMER_RIGHT_3_PENALTY=0.182645
PRIMER_LEFT_3_SEQUENCE=CCTTTGCTGCCTCTCTTTGTAT
PRIMER_RIGHT_3_SEQUENCE=AAACCCCCAGAATTCATCTTCT
PRIMER_LEFT_3=1146,22
PRIMER_RIGHT_3=1297,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=60.183
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=0.3187
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.182645
PRIMER_LEFT_4_SEQUENCE=TAGGAAACCTGTTGATGGGTCT
PRIMER_RIGHT_4_SEQUENCE=AACCCCCAGAATTCATCTTCTT
PRIMER_LEFT_4=1012,22
PRIMER_RIGHT_4=1296,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=60.183
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_04834 Ps_contig_mira-and-tgicl-ass_franssen_34764
SEQUENCE=ACNTYATTCAACCTCCTCAATCTTTACATCTAGAGTACAATACAACAATGATTCAGACCACAAGAATAGCAAACTCAGTTGCATTACAATCAAACAATAGTACAACCAACAGCACAAGATAATTATACTGATAAACAAAGTTAAAACAGACAACTAATCTACATGAAAAATATACATAAACTTAAAAACAGTAACTAAGGGGCAAAAACCCATAGCTATAAAGAATCTATAATTCAAACAGGAACTATGCTTACAGCTAGAGTATAAAGCTATAACAGATAAAATAATATCGAAACACTATTACCCATATACTATACAGAGATACAGCAAGGGTTATCTTATCTTATAATCATCACTTTGAAACTAGAAGCATTTCCGGTTTCTTGGCTTTGGTAGGTGTGTTGACATTTGAAGCAGCTGCAGAAGAAGCTGATTTTGCAGCATCCCTCGTCCTGTTATCGAGCTGAATGAGACTTCTTGTAGCAAGTATTGCTTGCGGCACTAAATCATGAGAAGCTCTTGCATACAAACACCTAACAGCAAAAGAAGCAGCATCCTTAACCTCTTGAGAATTCAATGGACCCATCAAACAATGTCCTAGAATTCTCAATGCAGGTTGAAGAATCTCCCAAGGTAAAGGGATTCTATCCCCTTTAGAATTTCCCTTTGGAGATTCACAATCCGGGTCATGATGAATCTCCAAGCTGTTCAGNGCTTCGGCAACCTCCTCAATCTCAGCATCACTCTCACCGTTCTCATGGCTTAAAGCTAGAGTGTTGTTGAGGTTATCGATTTGATCGAATTCTCTCCGGCAGGGGCAGTCCTGACCGGCCCAACCCGCGGCGAATAGACAGAATTCAAGCTTTGACCAGGAAGGCATATGGGAGATCTGGGAGAAGTAAGAATGAAGAGCGACACCGACGATGCAGGCGCGTTTGGTCGATTTAACGGCGAGATGAGGTTCGAGAGGAGGGGAGATAACACCGACGGAGGGAGTAGGGTTTGGGGAATGGGGTTTACGGAGGGGAGCATGGTAGATGGAGGGAATCGATAGATCGGGGATGGTAACAAGGAGAGGTTTACCGGCTCTCGATTTGGTTTCGGCGGCGTAGAGAGCGAGGAGGACAGCTTCGAATCCGGCGAGGGAAGGGGGATCGGAGGAATGGACACGGGAGAGGTAAAGTCCGGAGAGGAGAGGGATAAAAGAGAGAACAACGAGACGGAGGTGAGGGTCAGAGGAGAGAAAAGTGTCGTAAAGCCAGTGACAGAGGGGATCGGAATGGGAACCGGAGAGGGAAGAAGAGAGGGCGGAGTAAGCGGTGGGGGAGGAGAGAAGGGAGAGAGCGGGGCGTTCGGAATCGGCGAGGGGGGAGAGAGTGTGGTTATGTTGAGGGAGGATGGAAGCGAGGGAGTGGATTCGGGAACGGGCTTTGGAGACGGATTCCCACCAAGAGTGCATAGGGTCGTTGGAGTTTCTGTTAGGGTTGTTGTTGTTGTTGTTGATGGTGGTGGTGGTTACGGAGGAGGAAGAAGAGATAGAATTGGAATTTGAGGAGCATTGTGGAGATGAATTGTTGTTGTTGCGGTGGTGTTGGTGAAACTCCATAATCGATCCCCGATGCTCCTCCGATGGATGTTAGTCTTAAAACTGAAAACTGTGACTCAATCATGTAACTGCGGCCAACATTGCCGTTTTTATTAACTAGTACTGGTATAACTTTACTGCTAGAATGCTGGTCTTCCTAATTTTTATTTATTTTTACATGATCCATTACCCGAC
TARGET=1484,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04835 Ps_contig_mira-and-tgicl-ass_franssen_34764
SEQUENCE=ACNTYATTCAACCTCCTCAATCTTTACATCTAGAGTACAATACAACAATGATTCAGACCACAAGAATAGCAAACTCAGTTGCATTACAATCAAACAATAGTACAACCAACAGCACAAGATAATTATACTGATAAACAAAGTTAAAACAGACAACTAATCTACATGAAAAATATACATAAACTTAAAAACAGTAACTAAGGGGCAAAAACCCATAGCTATAAAGAATCTATAATTCAAACAGGAACTATGCTTACAGCTAGAGTATAAAGCTATAACAGATAAAATAATATCGAAACACTATTACCCATATACTATACAGAGATACAGCAAGGGTTATCTTATCTTATAATCATCACTTTGAAACTAGAAGCATTTCCGGTTTCTTGGCTTTGGTAGGTGTGTTGACATTTGAAGCAGCTGCAGAAGAAGCTGATTTTGCAGCATCCCTCGTCCTGTTATCGAGCTGAATGAGACTTCTTGTAGCAAGTATTGCTTGCGGCACTAAATCATGAGAAGCTCTTGCATACAAACACCTAACAGCAAAAGAAGCAGCATCCTTAACCTCTTGAGAATTCAATGGACCCATCAAACAATGTCCTAGAATTCTCAATGCAGGTTGAAGAATCTCCCAAGGTAAAGGGATTCTATCCCCTTTAGAATTTCCCTTTGGAGATTCACAATCCGGGTCATGATGAATCTCCAAGCTGTTCAGNGCTTCGGCAACCTCCTCAATCTCAGCATCACTCTCACCGTTCTCATGGCTTAAAGCTAGAGTGTTGTTGAGGTTATCGATTTGATCGAATTCTCTCCGGCAGGGGCAGTCCTGACCGGCCCAACCCGCGGCGAATAGACAGAATTCAAGCTTTGACCAGGAAGGCATATGGGAGATCTGGGAGAAGTAAGAATGAAGAGCGACACCGACGATGCAGGCGCGTTTGGTCGATTTAACGGCGAGATGAGGTTCGAGAGGAGGGGAGATAACACCGACGGAGGGAGTAGGGTTTGGGGAATGGGGTTTACGGAGGGGAGCATGGTAGATGGAGGGAATCGATAGATCGGGGATGGTAACAAGGAGAGGTTTACCGGCTCTCGATTTGGTTTCGGCGGCGTAGAGAGCGAGGAGGACAGCTTCGAATCCGGCGAGGGAAGGGGGATCGGAGGAATGGACACGGGAGAGGTAAAGTCCGGAGAGGAGAGGGATAAAAGAGAGAACAACGAGACGGAGGTGAGGGTCAGAGGAGAGAAAAGTGTCGTAAAGCCAGTGACAGAGGGGATCGGAATGGGAACCGGAGAGGGAAGAAGAGAGGGCGGAGTAAGCGGTGGGGGAGGAGAGAAGGGAGAGAGCGGGGCGTTCGGAATCGGCGAGGGGGGAGAGAGTGTGGTTATGTTGAGGGAGGATGGAAGCGAGGGAGTGGATTCGGGAACGGGCTTTGGAGACGGATTCCCACCAAGAGTGCATAGGGTCGTTGGAGTTTCTGTTAGGGTTGTTGTTGTTGTTGTTGATGGTGGTGGTGGTTACGGAGGAGGAAGAAGAGATAGAATTGGAATTTGAGGAGCATTGTGGAGATGAATTGTTGTTGTTGCGGTGGTGTTGGTGAAACTCCATAATCGATCCCCGATGCTCCTCCGATGGATGTTAGTCTTAAAACTGAAAACTGTGACTCAATCATGTAACTGCGGCCAACATTGCCGTTTTTATTAACTAGTACTGGTATAACTTTACTGCTAGAATGCTGGTCTTCCTAATTTTTATTTATTTTTACATGATCCATTACCCGAC
TARGET=1504,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04836 Ps_contig_mira-and-tgicl-ass_franssen_34764
SEQUENCE=ACNTYATTCAACCTCCTCAATCTTTACATCTAGAGTACAATACAACAATGATTCAGACCACAAGAATAGCAAACTCAGTTGCATTACAATCAAACAATAGTACAACCAACAGCACAAGATAATTATACTGATAAACAAAGTTAAAACAGACAACTAATCTACATGAAAAATATACATAAACTTAAAAACAGTAACTAAGGGGCAAAAACCCATAGCTATAAAGAATCTATAATTCAAACAGGAACTATGCTTACAGCTAGAGTATAAAGCTATAACAGATAAAATAATATCGAAACACTATTACCCATATACTATACAGAGATACAGCAAGGGTTATCTTATCTTATAATCATCACTTTGAAACTAGAAGCATTTCCGGTTTCTTGGCTTTGGTAGGTGTGTTGACATTTGAAGCAGCTGCAGAAGAAGCTGATTTTGCAGCATCCCTCGTCCTGTTATCGAGCTGAATGAGACTTCTTGTAGCAAGTATTGCTTGCGGCACTAAATCATGAGAAGCTCTTGCATACAAACACCTAACAGCAAAAGAAGCAGCATCCTTAACCTCTTGAGAATTCAATGGACCCATCAAACAATGTCCTAGAATTCTCAATGCAGGTTGAAGAATCTCCCAAGGTAAAGGGATTCTATCCCCTTTAGAATTTCCCTTTGGAGATTCACAATCCGGGTCATGATGAATCTCCAAGCTGTTCAGNGCTTCGGCAACCTCCTCAATCTCAGCATCACTCTCACCGTTCTCATGGCTTAAAGCTAGAGTGTTGTTGAGGTTATCGATTTGATCGAATTCTCTCCGGCAGGGGCAGTCCTGACCGGCCCAACCCGCGGCGAATAGACAGAATTCAAGCTTTGACCAGGAAGGCATATGGGAGATCTGGGAGAAGTAAGAATGAAGAGCGACACCGACGATGCAGGCGCGTTTGGTCGATTTAACGGCGAGATGAGGTTCGAGAGGAGGGGAGATAACACCGACGGAGGGAGTAGGGTTTGGGGAATGGGGTTTACGGAGGGGAGCATGGTAGATGGAGGGAATCGATAGATCGGGGATGGTAACAAGGAGAGGTTTACCGGCTCTCGATTTGGTTTCGGCGGCGTAGAGAGCGAGGAGGACAGCTTCGAATCCGGCGAGGGAAGGGGGATCGGAGGAATGGACACGGGAGAGGTAAAGTCCGGAGAGGAGAGGGATAAAAGAGAGAACAACGAGACGGAGGTGAGGGTCAGAGGAGAGAAAAGTGTCGTAAAGCCAGTGACAGAGGGGATCGGAATGGGAACCGGAGAGGGAAGAAGAGAGGGCGGAGTAAGCGGTGGGGGAGGAGAGAAGGGAGAGAGCGGGGCGTTCGGAATCGGCGAGGGGGGAGAGAGTGTGGTTATGTTGAGGGAGGATGGAAGCGAGGGAGTGGATTCGGGAACGGGCTTTGGAGACGGATTCCCACCAAGAGTGCATAGGGTCGTTGGAGTTTCTGTTAGGGTTGTTGTTGTTGTTGTTGATGGTGGTGGTGGTTACGGAGGAGGAAGAAGAGATAGAATTGGAATTTGAGGAGCATTGTGGAGATGAATTGTTGTTGTTGCGGTGGTGTTGGTGAAACTCCATAATCGATCCCCGATGCTCCTCCGATGGATGTTAGTCTTAAAACTGAAAACTGTGACTCAATCATGTAACTGCGGCCAACATTGCCGTTTTTATTAACTAGTACTGGTATAACTTTACTGCTAGAATGCTGGTCTTCCTAATTTTTATTTATTTTTACATGATCCATTACCCGAC
TARGET=1572,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04837 Ps_contig_mira-and-tgicl-ass_franssen_34769
SEQUENCE=ATGCCAATCTCAAGGAACTTTGGATTAACTCCATGAACGTTACAGACGATGGACCCAGGGTAATCCTGAGTGGCAGCAGATAGTCTGATTACCTTGTGACAGTATATACCAACAACCCAGTTGTTTAAATATTTGAGTAAAAATGCTTGCAGTTTTCCATTCTTCTCTCTTTTCACATATTCTAAATAGTTTGTGTGAATAATTCCTATAACTAGCCTGAATTTAGTTTTCCATCTTTTCCCATGGTGATACCAAGTCAGATGCTCAGGCTCCTCTAGAACAGCAATATCTGCATCTTCATCGGGGATAATTTCTGAAATATCGCCAACAGCAAGAATGCTCCTTTTATCTCTAGAAAACTTTCCCGGATAAAACTTTATGCTGAAACCAGATGCAAATCCAAGTCTATCCTCAAGCCATTGGCGGATATATTTCTCCTGCTCTTCCGGAGAATCAAATGTCATATTGTTTGGATATACTACTCGTTGATCTTTCAAAGATAACCAAGGGATCAACAAGGTGACATTCCTTTCCCCAGCTTTGTAAAGATACGCCGCACGAAAGAGAGGATTCACTGCGGTCCCGGTCAGCCACGGAAGGCTAGCAGTTGTAAAAATTGCAAAATGCTGTTTCTTATCCATCTGTTTGTTGGATTTAAAACTACAATATCATAGTAGTTCAGAACTCCCTTGAAGGATTGAAGATGAAGGTTAGAGAGAGAGAGAGGNANTTGTGTGNATNGA
TARGET=713,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04838 Ps_contig_mira-and-tgicl-ass_franssen_34772
SEQUENCE=GTCGGGGNACACATATAGAGTAACCCAACCCATTTCATCTCATTCTTCTTTCTTCTTCCATTTTCCATACCCCAAAGAAAATGAAAATGCCATCTTTTCTAGATTCTTTACCTCAACGTTCATTCTTCTTTTGTTTCCTCCTTCTTCCACTTTTGCTTTTACTCTTGCTCTTAGTCCCACCTTCATCTTCTTCTTCTTCAACTTTATCTTCATCCTCTTCAAGTTCCTCTAATGAAGTAACCATGTCAATCTACTATGAAAGTTTGTGTCCTTATTGTGCTGATTTCATAGTGAATCATCTTGTGAAACTCTTCCAAACAAATCTCATCTCCAATGTTAATCTTAGATTGATCCCTTGGGGTAATGCTTTGATTGCACCTGATGGCACTTTTGATTGTCAGCATGGAAATGATGAATGCTTTCTAAACACGATCGAGGCCTGCACCATTAAAATATATCCTGATGCGGGACAGCATTTCAGATTTATACACTGTTTGGAGCGTCTGACTTTAGAGAATCGTCATAAGGAATGGTTTAATTGCTTCCAAATGACAGGCTTGACCACAATGCCTATTGATTGCTACAAAAATGGCAATGGAAAATCTATTGAACAAAAGTTTGCTAAGGAAACAGCTGACCTCAATCCTCCTCATAATTTTGTTCCATGGGTGGTTATCAACAACCAAGCACTCAAAGAGGACTACTTAAATTTTGTGGCATATATTTGCAAGGCATACAAGGGAAATTTGAAACCAGATGCTTGTGGATCAGTTTCAACAAAAACTTATTATGATTCAGATGACAAAAATCAACTCTTCTCATCATGTTTGTTATATAGATGAAGCCAAAAATCTCACCTTACTAGGTACTAGTCACCAAATTAAGGTGTAAATTCCTATGACAAATGAAGTTGGAATGCATTTTTGATAATTTTCATGTGGAAATGCAAATGATTATGATAG
TARGET=186,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2526
PRIMER_LEFT_PENALTY=0.114470
PRIMER_RIGHT_PENALTY=0.138114
PRIMER_LEFT_SEQUENCE=TCTTGCTCTTAGTCCCACCTTC
PRIMER_RIGHT_SEQUENCE=TCAGGTGCAATCAAAGCATTAC
PRIMER_LEFT=162,22
PRIMER_RIGHT=381,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=60.138
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.2685
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.138114
PRIMER_LEFT_1_SEQUENCE=TTGCTCTTAGTCCCACCTTCAT
PRIMER_RIGHT_1_SEQUENCE=TCAGGTGCAATCAAAGCATTAC
PRIMER_LEFT_1=164,22
PRIMER_RIGHT_1=381,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.138
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=218
PRIMER_PAIR_PENALTY_2=0.4026
PRIMER_LEFT_2_PENALTY=0.264444
PRIMER_RIGHT_2_PENALTY=0.138114
PRIMER_LEFT_2_SEQUENCE=TTTCCTCCTTCTTCCACTTTTG
PRIMER_RIGHT_2_SEQUENCE=TCAGGTGCAATCAAAGCATTAC
PRIMER_LEFT_2=133,22
PRIMER_RIGHT_2=381,22
PRIMER_LEFT_2_TM=59.736
PRIMER_RIGHT_2_TM=60.138
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.4026
PRIMER_LEFT_3_PENALTY=0.264444
PRIMER_RIGHT_3_PENALTY=0.138114
PRIMER_LEFT_3_SEQUENCE=CTTTTGTTTCCTCCTTCTTCCA
PRIMER_RIGHT_3_SEQUENCE=TCAGGTGCAATCAAAGCATTAC
PRIMER_LEFT_3=127,22
PRIMER_RIGHT_3=381,22
PRIMER_LEFT_3_TM=59.736
PRIMER_RIGHT_3_TM=60.138
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.4401
PRIMER_LEFT_4_PENALTY=0.114470
PRIMER_RIGHT_4_PENALTY=0.325585
PRIMER_LEFT_4_SEQUENCE=TCTTGCTCTTAGTCCCACCTTC
PRIMER_RIGHT_4_SEQUENCE=TTACCCCAAGGGATCAATCTAA
PRIMER_LEFT_4=162,22
PRIMER_RIGHT_4=363,22
PRIMER_LEFT_4_TM=59.886
PRIMER_RIGHT_4_TM=59.674
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_04839 Ps_contig_mira-and-tgicl-ass_franssen_34782
SEQUENCE=AAGCAGGGGTTTCGAACGCAGAGTACGNGGAAACACACACAGAGAAACTCTCTTTCGTTCCTCTCTCTCACTTCATTTTCTCTCTCTAAACTTCAAAAGAAACCACAAAAATGGGATTCCCCATGGGGTACCCTGAAGTTTCCGTCCCCAACCTCTTCCTTCACCTTCTCTCTCTCCTCGCCTTTCTAAGATCTCTAGCCATCTCCTTCCTCTCTCTCCTCCATCTCTCCGACCTCCTCGACACCGATTTCTCCTCCGCCACCTTAACGGAATCACACCTCCACCGTCCTACCCTCTCCGCAATCCTCATCCGCGAATTCCTCCCCGTCGTCAGTTTCCGTGACCTCGCCGGAGATTCGAAAGCGATTGGATGTGCGGTGTGTCTCGACGAATTTTCCGGAGAGGAGGAAATCAGGTGTATGGCGAACTGTAGACATATTTTTCACCGGTCGTGTGTGGACCGTTGGATTGATCATGATCAGAAGACGTGTCCGCTTTGTAGGACCCACTTTGTACCATATCTTAAAATGGAAGATTATAATCAACGGTTATGGGCGGCTTGTGAATCTGAAAGTGAAGATGATGATGTTTCTCTTTTTCCTCAACATGATGATTATCACTACATTGCCACTGCTTCAATTTTGTTAGTTTTTTTTTTTTTTAACTTTTTTAATTTTTTTTTACCTTAATTATTTTTTATTAGGTTAGTTTT
TARGET=167,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3435
PRIMER_LEFT_PENALTY=0.006468
PRIMER_RIGHT_PENALTY=0.337062
PRIMER_LEFT_SEQUENCE=AGTACGNGGAAACACACACAGA
PRIMER_RIGHT_SEQUENCE=AGGAGAGAGAGGAAGGAGATGG
PRIMER_LEFT=21,22
PRIMER_RIGHT=219,22
PRIMER_LEFT_TM=60.006
PRIMER_RIGHT_TM=60.337
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.3435
PRIMER_LEFT_1_PENALTY=0.006468
PRIMER_RIGHT_1_PENALTY=0.337062
PRIMER_LEFT_1_SEQUENCE=AGTACGNGGAAACACACACAGA
PRIMER_RIGHT_1_SEQUENCE=ATGGAGGAGAGAGAGGAAGGAG
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=223,22
PRIMER_LEFT_1_TM=60.006
PRIMER_RIGHT_1_TM=60.337
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.3435
PRIMER_LEFT_2_PENALTY=0.006468
PRIMER_RIGHT_2_PENALTY=0.337062
PRIMER_LEFT_2_SEQUENCE=AGTACGNGGAAACACACACAGA
PRIMER_RIGHT_2_SEQUENCE=AGATGGAGGAGAGAGAGGAAGG
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=225,22
PRIMER_LEFT_2_TM=60.006
PRIMER_RIGHT_2_TM=60.337
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.3435
PRIMER_LEFT_3_PENALTY=0.006468
PRIMER_RIGHT_3_PENALTY=0.337062
PRIMER_LEFT_3_SEQUENCE=AGAGTACGNGGAAACACACACA
PRIMER_RIGHT_3_SEQUENCE=ATGGAGGAGAGAGAGGAAGGAG
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=223,22
PRIMER_LEFT_3_TM=60.006
PRIMER_RIGHT_3_TM=60.337
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=0.3435
PRIMER_LEFT_4_PENALTY=0.006468
PRIMER_RIGHT_4_PENALTY=0.337062
PRIMER_LEFT_4_SEQUENCE=AGAGTACGNGGAAACACACACA
PRIMER_RIGHT_4_SEQUENCE=AGATGGAGGAGAGAGAGGAAGG
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=225,22
PRIMER_LEFT_4_TM=60.006
PRIMER_RIGHT_4_TM=60.337
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=207
=
PRIMER_SEQUENCE_ID=SSR_04840 Ps_contig_mira-and-tgicl-ass_franssen_34783
SEQUENCE=AGGGAACGAAAAAGAAACATATTTAACCTTACCTCACTTATCTTTTACCCTATTTTAATCCGTACACCGAAATCCAATCNACAGTTTTCGCCCCCTTTTCTTCATCCATCTTAAATACCCTCTCCTCTCTCCAATCTCTCTGATCATTTTCTCTCCCACACCCACCCACCCAAACAAACCGTCGTCATCAATCAAAATGGCCGGTTTCATCTCAAAATCCAAAACCCTCATCCCCTCCAAACTCCCCAATCCATCCAATTTCATGGCGTCGTCACTCCGCTACCGCTCATCACGCGCCGCCACCAAACCCCAGTTATTGGAAATCGACACATCGTCCACATCATTATCATCGGAGGGTGAACACGAGATGACACTGAAGCTGTTTGATGATTTGATCCACCGAATTCTGGTGAAAAAAGCTACGCCAGATTGGTTGCCTTTTGTTCCTGGATCTTCTTTTTGGGTCCCACCACGACCTACTCCGTCTAATGTTGTTCATTTGGTTCATAAGCTTACTGATGCGGAAAAGCAACAACCTTTTGTTAACGATGAGTCGCTTTCGCTTTCTTCTCTTCGTGGTTGGCCTTCCTCCAATTACTTCATTAAAGGGAATGTTCATGGTGGGGATAGTGGTGTGGAGTTGAATATCCCAGAAGGGATGGAGGGACCAGTAAAAGTGAAGGTTATGACGCTTCCAGAACATTTAGCTCATTCCGAAGACGAGGATGAACCATAACTTAGTTCAGCCAGTTGCTCTGGGCTTGAATTCATGACCTCCATTTCCTTTAACTCTTAA
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1808
PRIMER_LEFT_PENALTY=0.009973
PRIMER_RIGHT_PENALTY=0.170825
PRIMER_LEFT_SEQUENCE=GTACACCGAAATCCAATCNACA
PRIMER_RIGHT_SEQUENCE=GACGATGTGTCGATTTCCAATA
PRIMER_LEFT=61,22
PRIMER_RIGHT=335,22
PRIMER_LEFT_TM=60.010
PRIMER_RIGHT_TM=59.829
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3250
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=6.75
PRIMER_PAIR_COMPL_END=2.75
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.2464
PRIMER_LEFT_1_PENALTY=0.075553
PRIMER_RIGHT_1_PENALTY=0.170825
PRIMER_LEFT_1_SEQUENCE=TTTAATCCGTACACCGAAATCC
PRIMER_RIGHT_1_SEQUENCE=GACGATGTGTCGATTTCCAATA
PRIMER_LEFT_1=53,22
PRIMER_RIGHT_1=335,22
PRIMER_LEFT_1_TM=60.076
PRIMER_RIGHT_1_TM=59.829
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.2892
PRIMER_LEFT_2_PENALTY=0.210152
PRIMER_RIGHT_2_PENALTY=0.079056
PRIMER_LEFT_2_SEQUENCE=ACCCTCTCCTCTCTCCAATCTC
PRIMER_RIGHT_2_SEQUENCE=GCTTCAGTGTCATCTCGTGTTC
PRIMER_LEFT_2=116,22
PRIMER_RIGHT_2=379,22
PRIMER_LEFT_2_TM=60.210
PRIMER_RIGHT_2_TM=59.921
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=264
PRIMER_PAIR_PENALTY_3=0.3089
PRIMER_LEFT_3_PENALTY=0.138114
PRIMER_RIGHT_3_PENALTY=0.170825
PRIMER_LEFT_3_SEQUENCE=TACACCGAAATCCAATCNACAG
PRIMER_RIGHT_3_SEQUENCE=GACGATGTGTCGATTTCCAATA
PRIMER_LEFT_3=62,22
PRIMER_RIGHT_3=335,22
PRIMER_LEFT_3_TM=60.138
PRIMER_RIGHT_3_TM=59.829
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3750
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=6.75
PRIMER_PAIR_3_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.3239
PRIMER_LEFT_4_PENALTY=0.138114
PRIMER_RIGHT_4_PENALTY=0.185822
PRIMER_LEFT_4_SEQUENCE=TACACCGAAATCCAATCNACAG
PRIMER_RIGHT_4_SEQUENCE=GAGGGTTTTGGATTTTGAGATG
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=228,22
PRIMER_LEFT_4_TM=60.138
PRIMER_RIGHT_4_TM=59.814
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3750
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_04841 Ps_contig_mira-and-tgicl-ass_franssen_34787
SEQUENCE=GAAGACGAGTGGTAGTCTAACGCGAGAGTACGTTCGGGGGTTTCGTTTTCCTTCTTCTTCTTCCTTYTYTCTCTCTCTGCAATTTTTTTTTCTAGTTACTTATTTTTTCGTTTGGCACTAGCGCTAGTTCTTGTCTTCTTTAGTCTCTGACCCTTTTGCCAGCTTTGACCTTAGAAGATGCTTGTGGCATTGCTCTTGCTTTTGGCATTGAGCAATGCCTCGAAAGTTTATGGAGATTTCGAGCAGTGTGGTTTGAATCCGAAATTGGATCCTAGACCACACACTGTCTCGATTTTGGAGTTCGGTGCGGTTGGTGATGGTAAGACACTGAATACACTTGCGTTACAGAATGCAATTTTCTATGTCAAGTCATTCGCTGATAAGGGCGGTGCTCAGCTCTATGTGCCACCGGGAAAATGGCTCACCGGAAGTTTCAATCTTACCAGCCATCTCACCCTCTTTTTAGAGAAAGGCGCTGTCATTATTGGATCTCAGGATCCATCTCATTGGGATGTTGTTGAACCTTTACCTTCGTATGGGCGAGGGAAAGATGTTCCTGAAGGAAGATACAAGAGTTTAATCAACGGTTACAAGTTGGAGGATGTGGTTATAACAGGAAATAACGGAACCATTGACGGCATGGGATCGGTTTGGTGGGAATGGTATAATTCTCACTCGCTCAACCATAGTCGTCCTCATTTGGTAGAAATTATATCATCCAATTCTGTCGTAGTTTCAAATCTTACATTCTTGAATGCTCCAGCATATAGCATTCACCCGGTTTATTGCAGCAATGTACATATTCAAAATGTTTCAATCTCCACTCCACCAGAATCCCCTTATACCGCCGGTATAGTACCAGATTCTTCTGATAATGTTTGTATAGAAGATAGTAATGTATCCATTGGCTATGATGCAATATCTCTCAAAAGTGGCTGGGATCAGTACGGCATAACCTATGGAAGACCGGCCGAGAAAATACACATAAGAAGGGTTCATCTTCACGCATTTTCCGGCTCTGCTATTTCATTCGGTACTGAAATGTCAGGTGGAATTTCAAACGTTCTAATCAAGCATGTTCACATTTTCAACTCGAATAGCGGCATTGAGTTCAGAACAATCAAAGGAAGAGGCGGTTACGTTAAACAAATCGTCATATCCAACATCAAAATGGAAAATGTTCATACAGCAATTTCAGCCAAAGGAAATTGTGGTTCTCACCCCGACGACAAATTCGATCCAAAAGCACTTCCACTTTTGAATCACATTACTTTGAAGGATATTATCGGAACAAATATCACAATTGCTGGAAACTTTAGCGGCATCGAAGAATCACCCTTCACCGATATCTGCCTTTCAAACATAACATTGTCCACAAATTCGGTTTCTTCCACTACATGGGAATGTTCAAATGTCTCCGGATTTTCAGACTCAGTGCTCCCTAAACCTTGTCAAGAACTCAGTAAACCTTCAAACTCTTCTTCCTCGTCGTGTTTCTATCTCATGAGTCTCGGTGAACAAAAATCTGCAGGGCTTTAATAAATCATTATGAAAACACCGAATTTGGCGCAAGTGGTTGGTGCACAGTGTTGTAAATTCTGAAGTACCGAGTTCGATTTCTGCTAAACAAAAAAATGAAAAAGTGAATCGCATCGCCGTTTGCCATCCGAGAATAGTCTACATTCTTTCCAAAATAAGACAGTTCATTTTGTTTCTTCCATTCCTTAGATTTGAATTCTCTTGCATATTCCCAAATAGGTCATTATTTGTACAGCTTCATTTCTATTTCTTCATTCATGTGTAATGAAATTTTGGTAGTGCCTTTAACAAAAGGACTCAAAATGTGATAGTTTACATTTTTGCATTAGTTTCATTGTTATTTAATTTCACTGTAAAGCATGTAGTGGATTTGGTGATACTTCAATTAAAGAAAGTTCAATTTTAAGTTARTATTAGTTTCATTGTTTAAAA
TARGET=51,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04842 Ps_contig_mira-and-tgicl-ass_franssen_34788
SEQUENCE=AAAGAAAACTGTTGTTTTAATTTGAGATCAACNCTNAAGTTTAARGTTTAATTACAAAACACTCCTGTAGTTCTTAATTTTGTGATTTTATACTTGCAACTTAAGTTGAATGAATTCTAGAAACATTTATACAATAAAATATAAGGCTTTTGTAGCTCAATAATCACCGCTATGATCAATCAGAACCTAACTGCTTGGGCCAGCTTCATCGTGATTCTTGCAACAAAATTCATTGTCACCGTGATCATGGGGATGTTTATCAGAGCCGTGTGGTACATCATCTTCATCACTATCATCTTCATCAAAAGGCTTCTCTTTATGAAAGATACAGCACTTCTTGGAACTCTTTTTCTGCATGAACTCATTGTCCACAGTGCCGTCTTTCCACGAGACCTTCTTCTTCTTGCGATTGAGGGGGAGAAAAAGGACTTCGGGTTGCTGCTGTTCCTGCTGAGAAGAAGAGGATGATGGAATTGAGCTTTCAATTGTTATGGTGGTGGTGCCAGTAACAGAAGGGGAAGACAACGCTACTTGTCGGGTGGTGCCTCTGCGTCTGTCCATGATTGGCAAAACAGAACTAGGAGAGACGCACGATTATGAGATCTTGACTGAGTAATTTCCAAGGTTGCTCTGTGTCTCTTTTACTCACCTTCGATTGAAGAGGCGGAGGAGATTCAGGTGTGGCCTTGCGCGGGAGAAGGAGACAAACGATGCGAAGATTGAAACGGCGGCGATTTTAGGTTGGTGACCAACGGTGAGAGAAAAACGGAATAAATGCACTCTGCTTTGTGATAACAATTTCTTATGAAGAGGAACAAAATAAAAGGATGAAGAGAATCCCCCGA
TARGET=394,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04843 Ps_contig_mira-and-tgicl-ass_franssen_34798
SEQUENCE=TAGTATTTCCAACTAACAAGGTTGGTAGATTTTACTTTTAAGAACRAGTGACCAAACAAAGTTCTTCTTCCATGTGACATGCTACATGTTTTTGCTCGTTCTCGTTCCTGTCACCCCTTTTTTCACCTAAACCGCAGCAGCCTCAGAATCCCTCCATCAAAATCACTTTCCAACCTTTCTTCATCCTCAACCCATCATCATCATCATCTTCCAATGGTGTCTCTTCTTGTTGCCACAACAATCGACCCGGCTTCAATCAACCCGGCCAATGCCCTGTTAGCATTTCCAGGCTGGCAACCCGGACCCCATTTCCAGGATGATATCAAGAGTTTTGTGAATGAAGGGTTGAGGGTTTTGCTTCATGGAAAGAGTATAGTTGTGGAAGATTATTTGGACGAAAAGTGGGAAAAGGTTACTGGAGAAGTTGTTGATGAAGTTATCTTCTTAAGTAAACACACTGCTGCTTCTAATAAACCTGCCCTCACTGTTCATCCGATTGGAGTTCCTCATTTGCGTGAAGGTGATGTTCCACCTCAAGGTGGGAAACCAGGATGGGCAGCACTGCCAAATCCTAGGATTGGACCTTGGATTCGACTTTTGAAAAATATTGCTCAAGCTCATAATCTTGTCCCTGAATTTGAGATTACTTTGGAGGCTACTCATCATGGACCTTTGACAAATAAGCCAACCATGTTTCTGGAGATTGGTAGTACTGAAGACTATTGGAAGAGACAAGATGCTGCTCAAGTTATGGCTCAGTTAGTTTGGGAAGGACTTGGACTTGGAGGAGGGACTGATGTTGGGAACTGGAGCAGGGCGAATGATAAGAAGAAAGTGCTTCTTGGGATAGGCGGTGGACATTATGTGCCTAGGCATATGGATGTAATATTGAAAGATGACGTTTGGGCTGGTCACCTACTTTCTGGCTACTCAGTGCCAATGGAAGATCCAAAAGGAGAAACAAATGTGGAGATTGGAGGAACTTGGAAACAATCTATAAAAGCTGCATATGAGGCCACTAAATCTGCTTTTCCCGGTGGCGAAATTCTTGCACATCTTGACCAAAAGAGCTTCAAGGGGTGGCAGAAGAATGCCATAACAGGATTCCTTGCAGAGCAGAATATTAAAATTGGAAAGCCAAATAACTTCTATTGAGTATCAATTTTAGAGGATGATATTGTGAATACTGAATACGTAGACTGCAGACAGAGGCAGACTNTAATTTTTTGTAGACCAGAATAGCAACTAAAAGTGACTTTGTTATGCAGTTAATTTAGAAAGTATGATTATTT
TARGET=193,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04844 Ps_contig_mira-and-tgicl-ass_franssen_34802
SEQUENCE=CTTCACNGAAAAAATTCAAAACTCTCATCCTCTAACTCGCAAACAAAACTTTAAAAGACAAAAAAAAACCTGACAGCAGTGTTACAAGAGGTGTAAACCAATTTCTCATATTAAAGTCGCCAACTAAAAGCACTAACAAATTAAAGTACTTGACCAAAGATTGCAATCCAGTTTAGTACAATGTATTTTTAAGGGAAAATGCACAGCTGAATCCTGAGCTATTACCAACAAACACCATCCACTTTCTCACTTGACTCGTTACAATTCTAATATATTTACCTCGAACAAAATTGGGATTCAGTCTAGTTTTCCGGTATGAATTACCGAGCCTTGAATGATAAAAGAATCAATTTCATCTTTGAAGAAACCACAAGTCAAATCACTCGTGTATGATTATTGTAACTAGAAACTCATCAATCAATCATGCAGTGGTGTCACCCAAAGAAGACCAATTATTCAGCTCTCAAATCTTGTATTCCCAATTATTTATCTACTGAAATAGTTTCCTCAACCTTTCAGCAGATGAAGCAGCCCTTTTGTTGGTTGGCTCAAGTACAAGGGCGTATTTAAAATCATCAATTGCTTCTTTGTAGTAGCCTAGCATCTCTCTAGCCGTACCTCTGCGAAAGTAGGCCTTCACATTCTTTTTGTCAAAACTTATTGCTGTTGTACAATCTTCCTCAGCTTGAAGATAACTGCCCAGTTCTAGATATGCTTGAGCCCTGTTACTGTAGTATGTTGCATTATTGCCGCAAAGTTTGATAGCTTCGGTATAAAATCCAATGGCCTTCTGCCATTGCTTATCTTTGTAGGCTTGATTTCCCTTTTCTTTCGAAATCTCAGCAGATTGTTCCTTGCTAACAACACTTTTTGAGGATTTACTTGGGGCAGCAATATCAGCCTGCTCTTGGAGAACTGTATACATGGTCTTAAGTGTGTCAAGCAAAAAGCGATCACCACCATGCCGAGCTATCAAGGACACTGAAACAGGATTCTTGTCAAAAAATCCCAATGGTACTGTGACCTGACAGCAACCTGATATGCTAGCAATACTTAGCAGGCTCAATGCACGGCTCTGATAATCATGAGATAGGAACTCCTTTCCACCTAATTTTGGAGGAGGATCAGCTACAGTAGGGATCACCAAAACTCCTTCGTCCTTCAATAATGAGTTTACAGCAACACGCAACTCACTTCTAACAGATTTAGAGTTTTCGATCTCTAATTCAGACACCTCAAACTTTTCATGCAATTGTGCTGAAACAGCTGGATGAAGATCAGGTTTTACTATGCTCATCCACTCACTATGTGTATGTTCAAATTCATGTCTTTGTAAAGACTGCATAACATTGGCGAGCAATTTTAATGAAGAAGATTTTAGTACGCCATTTGATTTTTGGATGGAACAAGCCTTCAGGCTGGAAACTTTAGAACTTATATAGTCTTCAAAATTAATATGCTTCAATACTTGCTTTCCAAAAAGCTTCTCAGTAGCTTTGATCACCACTTGAGAAGTCCTGTCAAGAGGAACATTTAGGTGTTGAAAACAGTCATCGGCTATAATTATTTGCCTGGGATTGCGTTGCATTACAAATGGTGCTTGTAAAAGTATATGCCCAACTCGACGCAATACATCAGGATCCTTTGCAAACCAACCAACAGTGTCCAGACTTGTTGAAACGGGTATGATTCCAACATGAGAAACAGCACCGTGTGAAGGTCGAAATCCAAGTATCCCACAGAATCCAGCAGGTACTCTCACCCCTCCAGAAGTATCAACGCCCAAAGAGAAATCGACGAAATTAGCAGCAACGGCAACGGCAGCACCACTAGATGAGCCACCCGGTACACGATTCGGAACAGCAGGATTGGTAGGCGTTCCGAAATGCTTATTTTCGCCACTAATGCCATAAGCCAATTCATCAACAACGGTAGTTCCAATACATGTAGCGCCGGATTCAACAAGAGCGGAGACAGCAGAAGCCGTAGAAGAAGCAGGTTCGTGCGTCCTTGCCCATTCCGGATGCCCGAACGTCGACACATGTCCCTCTATATCAAAAATATCGGAAATGGCGAAATTGAGGGAGGAGAGTGGGTGAGGAGCTTTGGGAGGAGCTGGAGGCGGTGGAGGAAGCAACTTAAGCTTTAGAAGAAAAGCGCCGAAATCTTCTTTAACGGCTTGTGTAAGCTTTCTGGTGAGAACGTAAATTCCGGCTAAACCCAAACCGAGTATTACCCATATTTGGCTCGACGGAGAAGCCATTGATTTCATGATAAATAAACTTGTTTGATGTGTGATTGTTATAAATGGAACGACGCTTCCTCTTCACACGCCGCCTCAAGTTCTTATGAGTTTTATCTATCTAACCTCTACTACTCTATCGCTTCTATCTCCTCGTACTAAGTACAAACCTTGGAGGTAT
TARGET=413,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4100
PRIMER_LEFT_PENALTY=0.331942
PRIMER_RIGHT_PENALTY=0.078029
PRIMER_LEFT_SEQUENCE=GGTATGAATTACCGAGCCTTGA
PRIMER_RIGHT_SEQUENCE=CTGCTGAAAGGTTGAGGAAACT
PRIMER_LEFT=312,22
PRIMER_RIGHT=521,22
PRIMER_LEFT_TM=60.332
PRIMER_RIGHT_TM=59.922
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.4100
PRIMER_LEFT_1_PENALTY=0.331942
PRIMER_RIGHT_1_PENALTY=0.078029
PRIMER_LEFT_1_SEQUENCE=ATGAATTACCGAGCCTTGAATG
PRIMER_RIGHT_1_SEQUENCE=CTGCTGAAAGGTTGAGGAAACT
PRIMER_LEFT_1=315,22
PRIMER_RIGHT_1=521,22
PRIMER_LEFT_1_TM=60.332
PRIMER_RIGHT_1_TM=59.922
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.5353
PRIMER_LEFT_2_PENALTY=0.331942
PRIMER_RIGHT_2_PENALTY=0.203343
PRIMER_LEFT_2_SEQUENCE=GGTATGAATTACCGAGCCTTGA
PRIMER_RIGHT_2_SEQUENCE=CTTGTACTTGAGCCAACCAACA
PRIMER_LEFT_2=312,22
PRIMER_RIGHT_2=558,22
PRIMER_LEFT_2_TM=60.332
PRIMER_RIGHT_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.5353
PRIMER_LEFT_3_PENALTY=0.331942
PRIMER_RIGHT_3_PENALTY=0.203343
PRIMER_LEFT_3_SEQUENCE=ATGAATTACCGAGCCTTGAATG
PRIMER_RIGHT_3_SEQUENCE=CTTGTACTTGAGCCAACCAACA
PRIMER_LEFT_3=315,22
PRIMER_RIGHT_3=558,22
PRIMER_LEFT_3_TM=60.332
PRIMER_RIGHT_3_TM=60.203
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.5735
PRIMER_LEFT_4_PENALTY=0.331942
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=GGTATGAATTACCGAGCCTTGA
PRIMER_RIGHT_4_SEQUENCE=ATCTGCTGAAAGGTTGAGGAAA
PRIMER_LEFT_4=312,22
PRIMER_RIGHT_4=523,22
PRIMER_LEFT_4_TM=60.332
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_04845 Ps_contig_mira-and-tgicl-ass_franssen_34805
SEQUENCE=TACGCAAGATGGCGGCTCACATTAAAGATTATAAATTAATAATACACTGTTTTCAAAAATATTTTATTTCATACAGTACAATGCATCAGTGGAATAATTTATTTCAATAAAGAAAGAGCAAACAAACAAGAATATTAATAAGCGGGAACATTTGAATCCAAAAAACTACCATCAAAATATTATTCAGATAGACTTCATTGCAAGAAGTGAGCATGTGTGAACTTTGAAGAATGCATTATTATATCACCAATGAATGCTTTCAAATCAGTGTCTGCTGAGCCACCATCTCCAATTGCATCACAACATATCTTTTGAAGCTTTCTACTTCTTTCCCTAATACCCTTTGTAACCTCACCATCCAAATCCATAAATTCAGACAATAATTTTACAATTTCACTTTTCTTCACCAAAACATCTCCCTTAACATTTTCTTTAACTCTCAACCCAACCTTCCAATCCTCTACTATCATCTTACTATTAAATGGTTGGTCAAAACTAATTGGAAAAGTCAACAACGGTACTCCAGAAATAACACTTTCCTTAGTAGAATTCCAACCACAATGTGACCAAAATCCACCAATAGAAGAATGTGACAACACTCTCAATTGGTCACACCAGTCCAAAACCAGTCCCATGTTTCCACAAATTTGTTTCAACCTTGAAGCTTCATTACGTGCTATCCACAAAAATCGAACATTGCTTTCACATAAAGCACTTGCAATTTCATCAACTTGTTCACTTGAAGCTGAAAAATAACTTCCCTGTGCTATGTAAAGAACAGAACCAATTGGTTGTGAATCTAGCCACTCAATGTAACTGTTGTTAGTGTTTGGCTTGGGATTACTTTCAAGGCTAATGTATGGTATGGTAGGACCAATAGTGTAAATTGGTATAGACAATTTTGATTTTAAGGTATCAATTGCTTGAGATTCTAGCTCATAAATTGAAGAAAATAGAAGATACTGTGCTTTGTGAAACCACTTAAAACCTTTCAAAAGCATCTGCATCAATCTTTTGTTTTGGTTACTATCATTGGAGTGAAAAAAAGGTAAATCAGCTAAGCGCGTTGAGGAAACTCCAGGAATATAATTTACACGTTCATCACCATTTTCTGAAATTTTCACAGAGTAGTGATCATTTTGTTGCAAAAGATGTTGGTGCAACAAAATAGAAAAAAAAGAAGCAGACGTTGTCCAAAATGATGCTACTGGTATATTCTTTCGGGTTCCGACGCCAACAACCCAATGCAAGAAAGTATCATACACAATGATTGAAGGAGGGTGGTCAAGTATGTTAAGTACCTCCTCAAACGGTTCCTCCATCTTTGTCATGATAGCTTCTATGAAGGCAAAATGGTCGCGGCTGCGAATGAGTTCGGAAGGAAGAACGTTAGGGATAGAACGGAAGCTTAAGTTGTTGGGTTTGGGCTCGGAGGAGATGAAGCTTAACCATTCTTCTGTGACAATAAAGGTTACAAGGATGTTGTTGTTGTTGGAGACAAGGAGCTTGCATAGGTTCAGCATGGGATTGATGTGGCCTCGTGCAGGATAAGGCATAGCAACGACGTGGTTGAAGGAAGTTGGTGGTGGTTCCATTGAAATTTTGGGAGAGGAATGGTAGTGAGTATTGATACTTTTGGGGAGAAGATAAGGTGGGGATAGATGAAGATTTGTGGCTTATTCTTGTTCTTTAAGGTGCTTATGTTGTCTTATTCCTTTAACGTG
TARGET=1481,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1678
PRIMER_LEFT_PENALTY=0.162217
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=GAACGGAAGCTTAAGTTGTTGG
PRIMER_RIGHT_SEQUENCE=ACTCACTACCATTCCTCTCCCA
PRIMER_LEFT=1398,22
PRIMER_RIGHT=1624,22
PRIMER_LEFT_TM=60.162
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.2227
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.086698
PRIMER_LEFT_1_SEQUENCE=TTGAAGGAGGGTGGTCAAGTAT
PRIMER_RIGHT_1_SEQUENCE=ACCTATGCAAGCTCCTTGTCTC
PRIMER_LEFT_1=1270,22
PRIMER_RIGHT_1=1515,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.3000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=0.2227
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.086698
PRIMER_LEFT_2_SEQUENCE=ATTGAAGGAGGGTGGTCAAGTA
PRIMER_RIGHT_2_SEQUENCE=ACCTATGCAAGCTCCTTGTCTC
PRIMER_LEFT_2=1269,22
PRIMER_RIGHT_2=1515,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.2479
PRIMER_LEFT_3_PENALTY=0.242357
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=AGCTTCTATGAAGGCAAAATGG
PRIMER_RIGHT_3_SEQUENCE=ACTCACTACCATTCCTCTCCCA
PRIMER_LEFT_3=1334,22
PRIMER_RIGHT_3=1624,22
PRIMER_LEFT_3_TM=59.758
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.2489
PRIMER_LEFT_4_PENALTY=0.162217
PRIMER_RIGHT_4_PENALTY=0.086698
PRIMER_LEFT_4_SEQUENCE=GAACGGAAGCTTAAGTTGTTGG
PRIMER_RIGHT_4_SEQUENCE=ACCTATGCAAGCTCCTTGTCTC
PRIMER_LEFT_4=1398,22
PRIMER_RIGHT_4=1515,22
PRIMER_LEFT_4_TM=60.162
PRIMER_RIGHT_4_TM=59.913
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=118
=
PRIMER_SEQUENCE_ID=SSR_04846 Ps_contig_mira-and-tgicl-ass_franssen_34809
SEQUENCE=CGTACACGACCGTTCCGTTACGTACGTAAAACGAAAAAACGAACGTAAGGAAGGANNTGTAGAGAGGAGGAAGGGAGGGCGCAATCTTTTGGAAAATCTTAACAACAGGGAAAGACAAATTAGGGAAATTGAAGCATCATTTGAAGCAGCCAAGTCACGCCCTGTTCATGCAACTAAAAAAGATTTGTATCCTGTTGAGTTCATGCCATTGTTGCCTGATATTGATAGGTATGATGATCAGTTTGTTCTTGCTGCATTTGATAATGCTCCAACAGCTGATTCAGAAATGTACAGGAAGTTGGACAAATCTGTTTGTGATATCTCTGAATCCAGGGCAGTTATGAAAAGTTATGTTGCAACAAGCTCAGATCCTGCAAATCCTGAGAAATTTTTGGCATACATGGTCCCAACACCAGGAGAGTTGTCAAAGGATATATATGATGAAGATGAAGATGTCTCTTATTCTTGGGTTCGCGAGTATCATTGGGATGTTCGAGGCGACGATGCACATGATCCCACAACATTCGTAGTTGCATTTAATGAGTCAGAAGCTCGATATTTGCCTCTTCCAACTAAACTTGTTTTAAGAAAAAAGAGGGCCACAGAGGGAAGATCAGGCGATGAGGTTGAGCAATTTCCAGCTCCCTCTAGAGTGACTGTGAGGAGAAGATCAAACGTTGCTGCAATTGAACGGAAAGATTCTGGGGTTTATGCAAGCTCAAAGGGCAATTCGTCAAAGAGCCTAGAAATGGATGACGATCTCGACGACGACGACCATAGAGATGCTGAGCACCAGGATAATTTCCAGTCTAGTGGAGCAGAAGATGACATGTCCGATTGATTCTTTGGCGCAGGTGTTGCTACATTAAGCAAATTTGTTCAATGAAGTTGGTGGTTGGATTGGCTGTGGATAGCTGAATTTGTATGTGTTTCAATGAAGGTTAAACAATGACAAATCTCAATTTTTTAATGACCATTTTAACCTTATGCATATACTTTCAGATTGTAGGCAGCTGTTAAATTAACAAAGATTTTGTTTTCAAATACGTTTATAATAGAAAAGTCGAAGAAATAAA
TARGET=763,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3422
PRIMER_LEFT_PENALTY=0.075753
PRIMER_RIGHT_PENALTY=0.266437
PRIMER_LEFT_SEQUENCE=ACGGAAAGATTCTGGGGTTTAT
PRIMER_RIGHT_SEQUENCE=GACATGTCATCTTCTGCTCCAC
PRIMER_LEFT=690,22
PRIMER_RIGHT=833,22
PRIMER_LEFT_TM=60.076
PRIMER_RIGHT_TM=59.734
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=0.3619
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.266437
PRIMER_LEFT_1_SEQUENCE=CTCGATATTTGCCTCTTCCAAC
PRIMER_RIGHT_1_SEQUENCE=GACATGTCATCTTCTGCTCCAC
PRIMER_LEFT_1=551,22
PRIMER_RIGHT_1=833,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=59.734
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=0.3619
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.266437
PRIMER_LEFT_2_SEQUENCE=TCGATATTTGCCTCTTCCAACT
PRIMER_RIGHT_2_SEQUENCE=GACATGTCATCTTCTGCTCCAC
PRIMER_LEFT_2=552,22
PRIMER_RIGHT_2=833,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=59.734
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.3829
PRIMER_LEFT_3_PENALTY=0.116503
PRIMER_RIGHT_3_PENALTY=0.266437
PRIMER_LEFT_3_SEQUENCE=GTCAGAAGCTCGATATTTGCCT
PRIMER_RIGHT_3_SEQUENCE=GACATGTCATCTTCTGCTCCAC
PRIMER_LEFT_3=543,22
PRIMER_RIGHT_3=833,22
PRIMER_LEFT_3_TM=59.883
PRIMER_RIGHT_3_TM=59.734
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.4016
PRIMER_LEFT_4_PENALTY=0.075753
PRIMER_RIGHT_4_PENALTY=0.325886
PRIMER_LEFT_4_SEQUENCE=ACGGAAAGATTCTGGGGTTTAT
PRIMER_RIGHT_4_SEQUENCE=TGCTTAATGTAGCAACACCTGC
PRIMER_LEFT_4=690,22
PRIMER_RIGHT_4=871,22
PRIMER_LEFT_4_TM=60.076
PRIMER_RIGHT_4_TM=60.326
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_04847 Ps_contig_mira-and-tgicl-ass_franssen_34816
SEQUENCE=CGGACGGGAAAAGTAAACAAAAAATAAAATTRGAAGTGATGGTAAAAAACAATGGGATCCAAGACCGAGACRACGCAAACAGATAGAGAGAGAAAGTGAGAAGAATTAGAGAGAGAGAGAGGTGAACACCGACCGGACGTTCGGTGCTCCGCTCATCACGGTTGTGGCCCCACCGCTCCGTATTTTACGTTCTAAAGGATCACATGTCAAACATAGAGGATAACAAAGAACTTGGACAGATGGATGTGGATGCATCTGGTGGTTCAGTTAACATGCCATCGCCCCAGAAGCAGGAGGAAACTTTAAAGAAAAAATATGGAGGAATGATTCCCAAAAAACCGCCACTTATTTCAAAGGACCATGAGCGTGCTTACTTCGATTCTGCAGATTGGGCATTGGGAAAGCAAGGCGGCGGTAAGCCTAAGGGACCACTTGAGGCTCTTCGGCCTAAACTACAGCCAACACAACAGCAAACACGTTACCGGAAATCTCCATACGCCCCTTCGGGTGAAGAAGGTGGAAGTGTTCCATCTGAGGATGCACCTTCCAATGAGTAAAAGCAGCAATCAAGCTTTTGATGATGTGCTGATTATTGTTCTGGTATACCTCAGTCTGAGGTATGGTATAACTTTTTGTTGTTTTCAACAATAAAATAAGCTTATTATTATTATTATGTATTCATATTAATTAGCACAAGACAAATTATTTGGCCTACAGCTATGCTGTAGACACTTTCTGTGACTGTGATTTGTGTTTTAATCTTCTTCCCCATCCCTTCATCTTTTTTATTTCCTTAGTTTTGAAAAGGAAAAAAAGTAAATAAGAAATTAATGATGGAAAGTGGTCATGATGTAAAAATTGAGTAGGCAAATATTAAACAAAGAATTAAAACAAAAATAGTTGTTGGT
TARGET=108,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04848 Ps_contig_mira-and-tgicl-ass_franssen_34816
SEQUENCE=CGGACGGGAAAAGTAAACAAAAAATAAAATTRGAAGTGATGGTAAAAAACAATGGGATCCAAGACCGAGACRACGCAAACAGATAGAGAGAGAAAGTGAGAAGAATTAGAGAGAGAGAGAGGTGAACACCGACCGGACGTTCGGTGCTCCGCTCATCACGGTTGTGGCCCCACCGCTCCGTATTTTACGTTCTAAAGGATCACATGTCAAACATAGAGGATAACAAAGAACTTGGACAGATGGATGTGGATGCATCTGGTGGTTCAGTTAACATGCCATCGCCCCAGAAGCAGGAGGAAACTTTAAAGAAAAAATATGGAGGAATGATTCCCAAAAAACCGCCACTTATTTCAAAGGACCATGAGCGTGCTTACTTCGATTCTGCAGATTGGGCATTGGGAAAGCAAGGCGGCGGTAAGCCTAAGGGACCACTTGAGGCTCTTCGGCCTAAACTACAGCCAACACAACAGCAAACACGTTACCGGAAATCTCCATACGCCCCTTCGGGTGAAGAAGGTGGAAGTGTTCCATCTGAGGATGCACCTTCCAATGAGTAAAAGCAGCAATCAAGCTTTTGATGATGTGCTGATTATTGTTCTGGTATACCTCAGTCTGAGGTATGGTATAACTTTTTGTTGTTTTCAACAATAAAATAAGCTTATTATTATTATTATGTATTCATATTAATTAGCACAAGACAAATTATTTGGCCTACAGCTATGCTGTAGACACTTTCTGTGACTGTGATTTGTGTTTTAATCTTCTTCCCCATCCCTTCATCTTTTTTATTTCCTTAGTTTTGAAAAGGAAAAAAAGTAAATAAGAAATTAATGATGGAAAGTGGTCATGATGTAAAAATTGAGTAGGCAAATATTAAACAAAGAATTAAAACAAAAATAGTTGTTGGT
TARGET=659,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04849 Ps_contig_mira-and-tgicl-ass_franssen_34836
SEQUENCE=CGGGGTGAGAGTCCATGGTTTAATCTGTTCATCCTTCGTCCTTCTCACACGCGTTTAACAACAACTCAACAGAACCGGAAGAGACCAAAGCCGAACTACGCCATAGGTCCTCGTCGTGCCAGATTTTGGCATCGGCGGCGGCGAGGCAAAGCGACAGCGTACCTTCACCGATGGCATATGACGACACGCGCGAGGACGAGTAAAATTGATTCAAAAATCGCGCAAGAAAATCGGATGAATCCGTGCCTTTCGAATTCCAAACGTAATCAATGCGCGAAATAGAAGCAATTTGAGGAAGACAAAAGACCTACCGGACTCGTCGATTCATTGTGTTGCGTTCATGGATGTTGATTCTTCAATTGGCCGTTGATGATGAAGATTGCTCGCGTGGTGTTGGCTCGGTGAAGATTGAAGAAGAAAGAGGAGGTCCAAAAAAAGAGGGAAAAGATTTGTGTACGTTTGGCTTATTGCAGCTTGTGGTTGAGTTTCGTCGTGCGTTGCCGTTTGCTTTGGAGCTGCTTTGTGTTGCAACGTCGTTTTGGATGCCATTTCATGTCGTTTGCAGCTTGCTATTTGTATTGCGTTGGTGTGGCTTGGTGTAGCAGCTTTGGTTTTCACGTACTCTTGCAGGTCGCATTGTCGGCGTCGCCGTTGCCGTTTGTGGCTTGGCTAGCTTTGTTTTCGCTGTTGTGTATGGTTGCGTTTGGGAATGTATTTCATTTTCGTTCTCGCCTGCGTTTCACGTGTTTTGCACTACGGTTCGCAACTAGTGCGTTTTGCTGCTTGAATTGCAGCTTGCGTTTCAATGTTGTTGTTGTTTGGATTCTTCCTTTTTGCTGTAAAATTGGATATGGATATAAAAAAAATGGAAAGTTGGATAACAAAATGGATTTACAAATTAGAATTGAATTATGGTTTTAAAGGA
TARGET=807,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.1094
PRIMER_LEFT_PENALTY=0.053944
PRIMER_RIGHT_PENALTY=4.055452
PRIMER_LEFT_SEQUENCE=TAGCAGCTTTGGTTTTCACGTA
PRIMER_RIGHT_SEQUENCE=CAGCAAAAAGGAAGAATCCA
PRIMER_LEFT=599,22
PRIMER_RIGHT=838,20
PRIMER_LEFT_TM=59.946
PRIMER_RIGHT_TM=57.945
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=4.2399
PRIMER_LEFT_1_PENALTY=0.184405
PRIMER_RIGHT_1_PENALTY=4.055452
PRIMER_LEFT_1_SEQUENCE=GCTTGCTATTTGTATTGCGTTG
PRIMER_RIGHT_1_SEQUENCE=CAGCAAAAAGGAAGAATCCA
PRIMER_LEFT_1=564,22
PRIMER_RIGHT_1=838,20
PRIMER_LEFT_1_TM=59.816
PRIMER_RIGHT_1_TM=57.945
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=4.3732
PRIMER_LEFT_2_PENALTY=0.317716
PRIMER_RIGHT_2_PENALTY=4.055452
PRIMER_LEFT_2_SEQUENCE=CTTGCTATTTGTATTGCGTTGG
PRIMER_RIGHT_2_SEQUENCE=CAGCAAAAAGGAAGAATCCA
PRIMER_LEFT_2=565,22
PRIMER_RIGHT_2=838,20
PRIMER_LEFT_2_TM=59.682
PRIMER_RIGHT_2_TM=57.945
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=4.3938
PRIMER_LEFT_3_PENALTY=0.338351
PRIMER_RIGHT_3_PENALTY=4.055452
PRIMER_LEFT_3_SEQUENCE=GCTTTGTTTTCGCTGTTGTGTA
PRIMER_RIGHT_3_SEQUENCE=CAGCAAAAAGGAAGAATCCA
PRIMER_LEFT_3=672,22
PRIMER_RIGHT_3=838,20
PRIMER_LEFT_3_TM=60.338
PRIMER_RIGHT_3_TM=57.945
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=167
PRIMER_PAIR_PENALTY_4=4.3942
PRIMER_LEFT_4_PENALTY=0.338748
PRIMER_RIGHT_4_PENALTY=4.055452
PRIMER_LEFT_4_SEQUENCE=TGGTTTTCACGTACTCTTGCAG
PRIMER_RIGHT_4_SEQUENCE=CAGCAAAAAGGAAGAATCCA
PRIMER_LEFT_4=608,22
PRIMER_RIGHT_4=838,20
PRIMER_LEFT_4_TM=60.339
PRIMER_RIGHT_4_TM=57.945
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_04850 Ps_contig_mira-and-tgicl-ass_franssen_34841
SEQUENCE=GCTTTCGTATAGAGATCCTGAAATGGCTAATTATGTTTCTCCTGAAATGTTTGTTGGATTTTCAGCTTCCAAAACGAATTGGATTGAAGCACAGAGAGTTCTCGCATGGAGCTTCAGCGACTCAAGGACTTCGAGGGAGTTAAACACGGCGAATTTACCAGTGTTCAAGCGAAAATCGGCGTCTTCACTTTCTGGCAAAGCGATTGCAGGGATTGCAGTTGGTTGTTTTGTTTTTGCTTTGAAGTTGTGCGAGTGTGTTTATATTATTATATCTTCGATGGCGAAAAAAACAAAAGAAAAGAAGAAGAAGATGACGTTGAAGATTGGGAAATGGAGTATTGGCCGCATAGATTCTCTTACGATGAACTAAGCATAGCCACAAAGGGTTTTCAA
TARGET=299,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2028
PRIMER_LEFT_PENALTY=0.120688
PRIMER_RIGHT_PENALTY=0.082113
PRIMER_LEFT_SEQUENCE=CTCAAGGACTTCGAGGGAGTTA
PRIMER_RIGHT_SEQUENCE=TAAGAGAATCTATGCGGCCAAT
PRIMER_LEFT=120,22
PRIMER_RIGHT=358,22
PRIMER_LEFT_TM=59.879
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.2467
PRIMER_LEFT_1_PENALTY=0.164560
PRIMER_RIGHT_1_PENALTY=0.082113
PRIMER_LEFT_1_SEQUENCE=GACTTCGAGGGAGTTAAACACG
PRIMER_RIGHT_1_SEQUENCE=TAAGAGAATCTATGCGGCCAAT
PRIMER_LEFT_1=126,22
PRIMER_RIGHT_1=358,22
PRIMER_LEFT_1_TM=60.165
PRIMER_RIGHT_1_TM=60.082
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.3254
PRIMER_LEFT_2_PENALTY=0.243298
PRIMER_RIGHT_2_PENALTY=0.082113
PRIMER_LEFT_2_SEQUENCE=CAAGGACTTCGAGGGAGTTAAA
PRIMER_RIGHT_2_SEQUENCE=TAAGAGAATCTATGCGGCCAAT
PRIMER_LEFT_2=122,22
PRIMER_RIGHT_2=358,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=60.082
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=0.3728
PRIMER_LEFT_3_PENALTY=0.120688
PRIMER_RIGHT_3_PENALTY=0.252154
PRIMER_LEFT_3_SEQUENCE=CTCAAGGACTTCGAGGGAGTTA
PRIMER_RIGHT_3_SEQUENCE=ATCGTAAGAGAATCTATGCGGC
PRIMER_LEFT_3=120,22
PRIMER_RIGHT_3=362,22
PRIMER_LEFT_3_TM=59.879
PRIMER_RIGHT_3_TM=59.748
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=12.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.4056
PRIMER_LEFT_4_PENALTY=0.120688
PRIMER_RIGHT_4_PENALTY=0.284927
PRIMER_LEFT_4_SEQUENCE=CTCAAGGACTTCGAGGGAGTTA
PRIMER_RIGHT_4_SEQUENCE=TGTGGCTATGCTTAGTTCATCG
PRIMER_LEFT_4=120,22
PRIMER_RIGHT_4=380,22
PRIMER_LEFT_4_TM=59.879
PRIMER_RIGHT_4_TM=60.285
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=261
=
PRIMER_SEQUENCE_ID=SSR_04851 Ps_contig_mira-and-tgicl-ass_franssen_34854
SEQUENCE=ATACTTTCTTGTAATAGGAGATGCAACCAGAGATGCAACTAAACCAGCGATGTAAAGAGAAGATGTAAAGGCAGCAAGACTCTGACTGTCGTATTTGCAGTAGTTATTCTCGTGTGCATTGTTTTTTTGCATGAATACTTCTGGGAAGAATTCTTCAAGGAAGTCATCCATGGAAGTCACCCCACCTGAAATTCCAATATCATATCCAAATAATGATCCTCCAATGCGGCAACAATGCAAGCAATGATAACATAAGGAGTAACTCTCCCTTTATAAAGATTTGCCCTTTTCTTCCCCACAGTATCTGTTGTAAAACCCCCACCTGCCATTTTCTTGATTCAGAGAAGAATGAATGCTTTTCTTTTTCTTTCTTTCAAAAAATAGAAAATATCATAG
TARGET=364,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04852 Ps_contig_mira-and-tgicl-ass_franssen_34860
SEQUENCE=TCGGGGCTCCAATTTCTTCTTCTTCAACTCTCATATGGCAGGAGAAGGGAAGAAGCAACCGAAAATTGTTTTGACGAAGCCATTCTCATTGGAAATTGAATCGGATTCAGAGCCGAGTTACAGTTACAGGGCCCCCAATATTGTTCGCCGCTTGTTAAGTTTATTTAAGAATGTACGCCCGGGAGCAGATCTCACTAACTTACAGCTTCCGCCTCTATTTTATTTCCCAAAATCACAACTTCAATGCTACGGTGAATCAGTATATAGCACAACTTCAAATTCAAACTTGCTGGCCAAATGCAACAACGAGCAAAGTCCATTGGAAAGGCTCACATCTGTCGTAGCATGGAGCATATCTACCACACGCCCTACATCTTTTGGCGTTGCTCCGTATAATCCCATTCTTGGAGAGACGCACCATGTTTCCAAGGGCAACCTTAACGTCCTACTCGAGCAAGTTTCCATCGATCCTCCGGTATCTGCTCTTCACG
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04853 Ps_contig_mira-and-tgicl-ass_franssen_34865
SEQUENCE=ACAGTGNNNGGAAACCACCACTATTTTTCTTCTTCTTCCCATCTTCTTTTTCACCCAATAAACCTAAACCTCTTATGGCAGATCCACAGAGAAACAATACCCATAACCAAACAAGCCGTTTACAGAAACGAGCTCCATCTTCCTTACAAATCAACCGCTCCCTCGATTGGAACGTCGCCATCCCCCTCCTCTCTCCGTTAGCATCTTCTCCGCCACCGCAACCGTCTCACGATCCATCGCAGCGCCATCAAGGACCCACCGCCGAGCCGGAGAAGGTCGTTGTCACTTTCAAAAACTGGCAGCACCCGGCAGCGCCTTTCTGCTACGAGCCAGCTCCGATGGCGCCGCCGTTTGTTCCGGTATAGAACACGAGACAGATTTAACGCTTTATTTCATATTTCGTAATAATTAGTCAGAAATCATATCGGAAAGATCTAACGGTCTATATTTATCTTTGCGTTTTTTTTATTCGTGGGGAAAAGTTTTTTTTCTTCTATTTTTCATGCTGGGTCTATGTTATGTTATGAATATACCTCAACACAGACCATGTGTGTTTGTTTCTATAATTTTTAGCTTTTCCCTATTTTACCCTTTTTAATATTTTATAATTTTGGGGTTTCAATTAGTAGAAATATCATTATGTTACCCCAATTTCTCTTAATTTTCCAGAAGATTCCATGTTGTGTATGTGTATAGTGTAATTAATCTCTAATTAAAAAAAATGGATGAGTGAGAGAAGATTCGTTGGTTTGTGATATTAGTGGATAAGATGCCACAAGAGAGGACCAGTTTTGTTTTGGTGCATAACAGCTCATGTTCTTTGTCCAAAAACTGCACATGCAGTGTTCAGAAATCAGAACAAAGATTAATTTATARTAAACC
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04854 Ps_contig_mira-and-tgicl-ass_franssen_34866
SEQUENCE=ACATGACTCGCTACGACGTAAAAAGCATCGCAAATAGTTCTCTTCCCATAGGCGGTTTATCGAATAAAAACAACAAATTTTCTACTGATTCTTTATCCGAAAGCAAAGGTCTCGAAGTCGCGAGTATATCAGATGAAAGAAATCAACCAACACCATTTACAATATCACAACAACCTTCAAGTTCTACCTTAAGTTTCGCCGTTCCGATTAAACAAGATCCTTCTTCAGATTACTGGTCCAATATTCTTGGATTCCATAGTNATAATAATCCTAGTAATGTTGTGGTTCCATCTACCACACCCTTCAACATGGATTTTTCTTCTCATGCAACACCTTCGAGTACAAACAGTGATAACAATGGAGGTTTCTTCAGTGGATTTGTTCAGCATCAACAAGATGGAAATAATGGAAGCAGTTCTTCATCTTCATCATCAACATCATCATCTTCAACAAGTTCAATTCCATTTGCTACACCAATATTTTCTTTAAGTAGTACAAATAGTTATGGAAATAACTGGATTAATAATGGACACGCATTCCAAACACATGCAAAACCAAGTCTGTTTCAAACACCAATATTCGGAATGGAATGAGGTCATGCATTCAGGTAGAGAAGAAGATGAGAATCTGTGCAAATAATAATAATAATAATAATGAATGATGAAAATGTTGAAGGACACAGTGATGGTAACATGCAATAGAAGCAAGAAAAAGAAGAAGGACTAGTCTTGAGTTAGTTTCATGAGATAGCTATATTGCTGACACTGYGTANTACTACTACTACTAACTNAAAGTTGTCTTTTTGGTTTTGTTTGATTCTTGTTA
TARGET=635,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04855 Ps_contig_mira-and-tgicl-ass_franssen_34866
SEQUENCE=ACATGACTCGCTACGACGTAAAAAGCATCGCAAATAGTTCTCTTCCCATAGGCGGTTTATCGAATAAAAACAACAAATTTTCTACTGATTCTTTATCCGAAAGCAAAGGTCTCGAAGTCGCGAGTATATCAGATGAAAGAAATCAACCAACACCATTTACAATATCACAACAACCTTCAAGTTCTACCTTAAGTTTCGCCGTTCCGATTAAACAAGATCCTTCTTCAGATTACTGGTCCAATATTCTTGGATTCCATAGTNATAATAATCCTAGTAATGTTGTGGTTCCATCTACCACACCCTTCAACATGGATTTTTCTTCTCATGCAACACCTTCGAGTACAAACAGTGATAACAATGGAGGTTTCTTCAGTGGATTTGTTCAGCATCAACAAGATGGAAATAATGGAAGCAGTTCTTCATCTTCATCATCAACATCATCATCTTCAACAAGTTCAATTCCATTTGCTACACCAATATTTTCTTTAAGTAGTACAAATAGTTATGGAAATAACTGGATTAATAATGGACACGCATTCCAAACACATGCAAAACCAAGTCTGTTTCAAACACCAATATTCGGAATGGAATGAGGTCATGCATTCAGGTAGAGAAGAAGATGAGAATCTGTGCAAATAATAATAATAATAATAATGAATGATGAAAATGTTGAAGGACACAGTGATGGTAACATGCAATAGAAGCAAGAAAAAGAAGAAGGACTAGTCTTGAGTTAGTTTCATGAGATAGCTATATTGCTGACACTGYGTANTACTACTACTACTAACTNAAAGTTGTCTTTTTGGTTTTGTTTGATTCTTGTTA
TARGET=773,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04856 Ps_contig_mira-and-tgicl-ass_franssen_34868
SEQUENCE=CTATTGTCAATTAATAATACCTCAACAAGCAAAACAATCGNTTGAAAATTTAGTTCTTTCTAAAATACCCNAGTTGCACTGGTAATATGAATTACAAATGCAGAATGCTTCTATATATTGTCACAAATGAATGAATACACATGGTTTAATCTATGAATTATATTTCAATTAAACTGTATTAACGAAATCCTATTAGGACAAAAAATATCTCTTCAATATATATTTTTCACTCATTCATTCCAGTTTCATCTTAAACTGACACGGTAAAGAGGTATATCTTTAAAACAACGGATAGAGCCAACTGCAGCAGAAAAATGCCAGCAACAATGAAGAACCACTGTTCCCCAGCTGACTTGATATGAGCCCGTAGGTTAAGTGCGACAAATGTCGCCGACATGAACCCTGCCACACCTGCAATCACCCATCTGAATATATCCAGTGGGACAATTGACATGCACAGAGCTGGAATAAAGATAAACAGGGAATATCCATATAAGCAGAATAGTTGAACAAGTCCCGCCGGTACAGAGAAGTACTTGAGGATTACATACAGGCAAAGAGGAACGATAGTAACATAGCCGTAAAACAAACCAGCAGACCAAGTCACCAGATTTATGTCATAGTTCCATTCCTGATCCTTCAGCTTGTGTGCTATATATGTCACAAATGTTCCCATAGATGCTGCTAGAAATATTAATGTAGTACAAATCCAGAATGGTCCATACAAATCAGGGTTGGTAGAAGTTTTTTCATTAAAAGTTCCTCTGAATGGAAAAAGTGAGTCTATAATCCTCTCTAGAACATCTGAAGTGTCGACATCAAAGTATGGTTTGTAAGAGGCAACTGCAAAAGTTTGAAGCCAACCACCCTGCTGAGGTTCATCAGAAGAACCACCAGATACAGGTTTTGAAAATGAATCATCTGCATCTCGAGGTGGTCTAGAACCACCGGTAATCTTCCCATGAGCTTGTGATGGAGGAAATGTCTGAAGATTTGAATCGGTGAATTTGACGGTGACAGGTGGTGGATCTGGAACGGCCGGAACAGATCCTTGTAACTGTTGATTATTAATCGTTGAATATTTTCCTCCTGATGTCATCATTTCTTCTTCTTCTGATCACAGATCCACAGATCTTATTTTTTCTTCTTTCTTCTTTCTTTTGTTCTACCTCTCTCAGTTTTCCCG
TARGET=1103,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1525
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.120688
PRIMER_LEFT_SEQUENCE=ATGAGCTTGTGATGGAGGAAAT
PRIMER_RIGHT_SEQUENCE=CGGGAAAACTGAGAGAGGTAGA
PRIMER_LEFT=961,22
PRIMER_RIGHT=1185,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=59.879
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.1525
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.120688
PRIMER_LEFT_1_SEQUENCE=AATCTTCCCATGAGCTTGTGAT
PRIMER_RIGHT_1_SEQUENCE=CGGGAAAACTGAGAGAGGTAGA
PRIMER_LEFT_1=952,22
PRIMER_RIGHT_1=1185,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=59.879
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.2311
PRIMER_LEFT_2_PENALTY=0.110392
PRIMER_RIGHT_2_PENALTY=0.120688
PRIMER_LEFT_2_SEQUENCE=TGTCTGAAGATTTGAATCGGTG
PRIMER_RIGHT_2_SEQUENCE=CGGGAAAACTGAGAGAGGTAGA
PRIMER_LEFT_2=982,22
PRIMER_RIGHT_2=1185,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.879
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=204
PRIMER_PAIR_PENALTY_3=0.3622
PRIMER_LEFT_3_PENALTY=0.241544
PRIMER_RIGHT_3_PENALTY=0.120688
PRIMER_LEFT_3_SEQUENCE=TGGTCTAGAACCACCGGTAATC
PRIMER_RIGHT_3_SEQUENCE=CGGGAAAACTGAGAGAGGTAGA
PRIMER_LEFT_3=934,22
PRIMER_RIGHT_3=1185,22
PRIMER_LEFT_3_TM=60.242
PRIMER_RIGHT_3_TM=59.879
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.5286
PRIMER_LEFT_4_PENALTY=0.407941
PRIMER_RIGHT_4_PENALTY=0.120688
PRIMER_LEFT_4_SEQUENCE=CTTGTGATGGAGGAAATGTCTG
PRIMER_RIGHT_4_SEQUENCE=CGGGAAAACTGAGAGAGGTAGA
PRIMER_LEFT_4=966,22
PRIMER_RIGHT_4=1185,22
PRIMER_LEFT_4_TM=59.592
PRIMER_RIGHT_4_TM=59.879
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_04857 Ps_contig_mira-and-tgicl-ass_franssen_34873
SEQUENCE=TAATTTTAATAGTTATTATCAATTCATCTTATTCGATTAAAAAATAAAAAAATAAGTTATAAAGTTTATAACAAACCTGAAGGTCAAGACGTTTTTCCCAGCCACTAGGAACCTGTAAATCAAGATCCAATTCTTGGGATTCATTGGAGGACCCACCGAGTAAGTCTCTTGTAATCAGAGCCGTTGATTTCTCACCGTGAGATTCATTTCCCACCGTCCGATGTTGTTCATCTTTGTATCCACCACCACCAAGAACACGAATTAGAGAACTAACTTCAGCAGCCATTGCTTAGTTTCGTTTTTCAAATAGTTCTAGAGAGAGAAACTTTCTAGAGAGAGAAACCAGAGAGAGATGAAGATGAGAAAGAGAAAAGTCTGTNTCCCCCGA
TARGET=240,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1849
PRIMER_LEFT_PENALTY=0.143968
PRIMER_RIGHT_PENALTY=0.040897
PRIMER_LEFT_SEQUENCE=CCTGAAGGTCAAGACGTTTTTC
PRIMER_RIGHT_SEQUENCE=TAAGCAATGGCTGCTGAAGTTA
PRIMER_LEFT=75,22
PRIMER_RIGHT=291,22
PRIMER_LEFT_TM=60.144
PRIMER_RIGHT_TM=60.041
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.2080
PRIMER_LEFT_1_PENALTY=0.167063
PRIMER_RIGHT_1_PENALTY=0.040897
PRIMER_LEFT_1_SEQUENCE=GACCCACCGAGTAAGTCTCTTG
PRIMER_RIGHT_1_SEQUENCE=TAAGCAATGGCTGCTGAAGTTA
PRIMER_LEFT_1=149,22
PRIMER_RIGHT_1=291,22
PRIMER_LEFT_1_TM=60.167
PRIMER_RIGHT_1_TM=60.041
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.2636
PRIMER_LEFT_2_PENALTY=0.222735
PRIMER_RIGHT_2_PENALTY=0.040897
PRIMER_LEFT_2_SEQUENCE=CAAGATCCAATTCTTGGGATTC
PRIMER_RIGHT_2_SEQUENCE=TAAGCAATGGCTGCTGAAGTTA
PRIMER_LEFT_2=120,22
PRIMER_RIGHT_2=291,22
PRIMER_LEFT_2_TM=59.777
PRIMER_RIGHT_2_TM=60.041
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=0.5223
PRIMER_LEFT_3_PENALTY=0.167063
PRIMER_RIGHT_3_PENALTY=0.355258
PRIMER_LEFT_3_SEQUENCE=GACCCACCGAGTAAGTCTCTTG
PRIMER_RIGHT_3_SEQUENCE=GGGGGANACAGACTTTTCTCTT
PRIMER_LEFT_3=149,22
PRIMER_RIGHT_3=385,22
PRIMER_LEFT_3_TM=60.167
PRIMER_RIGHT_3_TM=59.645
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.50
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=0.5659
PRIMER_LEFT_4_PENALTY=0.524968
PRIMER_RIGHT_4_PENALTY=0.040897
PRIMER_LEFT_4_SEQUENCE=CCAGCCACTAGGAACCTGTAAA
PRIMER_RIGHT_4_SEQUENCE=TAAGCAATGGCTGCTGAAGTTA
PRIMER_LEFT_4=97,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.525
PRIMER_RIGHT_4_TM=60.041
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_04858 Ps_contig_mira-and-tgicl-ass_franssen_34876
SEQUENCE=AAAATAACAAATTTTATATTTGATGAATTGAATACACTAACAATAATGTTACAAAATGTCTTTCTAATGAACATCACATGTGATACGCTACAACAAATGTCTACCTCAAAGATTACATAAATTATCATACAAACTATGAACTTCAAAAGCCAAATTCATATGAATTGGTTAGCACTTCAATTATGTCCCAAAATAGGTTACAATTATACCAAACAACATCTTGTAGGATCTAGAAAAACTCCAACTATGTCATCAAGTGATGAATACCCTTAACTGTTTCACATTTCTCTGTCAATTATATCTCTCATCATTTGTATTGGTATAAGCCCTTCAGTTCTAATAGCATCTTTCTTTGGATCAGGACTTATAAAGAATAGGGTTGGCAACCCTCTAACCTGCATGTCACGCGCGAATTCATACTCGTCATCTGTATCAACCTTAACGATCATCACATTTTTCTCGTACTCGACTGCAAGCATTTCAATTTCTTGTGCCATTAAGATGCACGGTCCGCACCACGTCGCGTAGAAATCAATAAGAATAGGAACATCTCTTTCTCCTTTCACCAGCTCCTGGATTTCTTCAGCTGAAAACTTTTTCACAAGATAGTCTTGTCTAACGTATTTTCCTTGAGGTGGTTTGCAAAGCAATTTTCTAGGGGGTGTAGAGATAGAAAGAGGTGTATTGTTGAAGTGAGTGGTTGTGGTGAGAGAATGAGGTTTGGAAGAGAAGGGTGAGAGAGTGGATAATAGTCTGTGATGTGGAAGAGTGTAGGAATGGAAGAGAGCCATGGATGAGTTTGGAGAGTGTGAGGAAGAAAAAGAGGGTTTTTGAATCACGNGTATTATCGTATCTATCTATCTTNTGTATTTTGAATTAGATAGAATTATCCAAAA
TARGET=851,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04859 Ps_contig_mira-and-tgicl-ass_franssen_34878
SEQUENCE=CGTGTCTCAGAAATGGAGGACAAGTTTGTCGTATTGTCTTCGCCTAGCATTGCAAATCCAACGGTGTTTCAAGGAGTGTTTTGCCGAGATCTAACGGTGGTAGGGAACTTGCTTTCTCAAAGGTTTAGTAGTAGGTCAATTCCGGTCAAATACGAGGCTCTTAGTTGTTGAATTCATTGATTGATTGGGGGACTTGGGATGTTTTGTTTTTTTACCGGCTGAAAATATCTCTGATTCTGATTATTGGTTAATTGTATGTACAAATGGATAAATTAATTTGAAAGTGCCGGGAGAAATTAATTAATTTNAAGGTT
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7044
PRIMER_LEFT_PENALTY=0.056850
PRIMER_RIGHT_PENALTY=0.647580
PRIMER_LEFT_SEQUENCE=ACGGTGTTTCAAGGAGTGTTTT
PRIMER_RIGHT_SEQUENCE=ATTAATTTCTCCCGGCACTTTC
PRIMER_LEFT=60,22
PRIMER_RIGHT=300,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=60.648
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=0.7044
PRIMER_LEFT_1_PENALTY=0.056850
PRIMER_RIGHT_1_PENALTY=0.647580
PRIMER_LEFT_1_SEQUENCE=AACGGTGTTTCAAGGAGTGTTT
PRIMER_RIGHT_1_SEQUENCE=ATTAATTTCTCCCGGCACTTTC
PRIMER_LEFT_1=59,22
PRIMER_RIGHT_1=300,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=60.648
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.8682
PRIMER_LEFT_2_PENALTY=0.220659
PRIMER_RIGHT_2_PENALTY=0.647580
PRIMER_LEFT_2_SEQUENCE=TTTGTCGTATTGTCTTCGCCTA
PRIMER_RIGHT_2_SEQUENCE=ATTAATTTCTCCCGGCACTTTC
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=59.779
PRIMER_RIGHT_2_TM=60.648
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.8853
PRIMER_LEFT_3_PENALTY=0.237699
PRIMER_RIGHT_3_PENALTY=0.647580
PRIMER_LEFT_3_SEQUENCE=GGTAGGGAACTTGCTTTCTCAA
PRIMER_RIGHT_3_SEQUENCE=ATTAATTTCTCCCGGCACTTTC
PRIMER_LEFT_3=98,22
PRIMER_RIGHT_3=300,22
PRIMER_LEFT_3_TM=59.762
PRIMER_RIGHT_3_TM=60.648
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.9398
PRIMER_LEFT_4_PENALTY=0.056850
PRIMER_RIGHT_4_PENALTY=0.882980
PRIMER_LEFT_4_SEQUENCE=ACGGTGTTTCAAGGAGTGTTTT
PRIMER_RIGHT_4_SEQUENCE=ATCAGAGATATTTTCAGCCGGT
PRIMER_LEFT_4=60,22
PRIMER_RIGHT_4=234,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=59.117
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_04860 Ps_contig_mira-and-tgicl-ass_franssen_34878
SEQUENCE=CGTGTCTCAGAAATGGAGGACAAGTTTGTCGTATTGTCTTCGCCTAGCATTGCAAATCCAACGGTGTTTCAAGGAGTGTTTTGCCGAGATCTAACGGTGGTAGGGAACTTGCTTTCTCAAAGGTTTAGTAGTAGGTCAATTCCGGTCAAATACGAGGCTCTTAGTTGTTGAATTCATTGATTGATTGGGGGACTTGGGATGTTTTGTTTTTTTACCGGCTGAAAATATCTCTGATTCTGATTATTGGTTAATTGTATGTACAAATGGATAAATTAATTTGAAAGTGCCGGGAGAAATTAATTAATTTNAAGGTT
TARGET=295,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04861 Ps_contig_mira-and-tgicl-ass_franssen_34880
SEQUENCE=GGTGCCGGGATTGCGCTTAATAAGTTTCTTAGTGTTCTNCTCAGCAGCCTATGCCGATTATGATGGATGAGATGCCGCCGCAGACTCCGGAGAATTCGGATTCCAACAAGTCGTGGTTTGGTGGAATCTTCGGTGGGGCCAAGGAAGAAGAGAAGAATACTAGTGGAGGAAGCGATGTTAAGATTCTCGAGAGTTTTGATGCTCCTCCTGTGCCTAATTTCGAGTACAAGTGAATTAGGGTTTGGTTTGGTTTTGGCCTATGAGGTAGTGATTTTTGTTATGTTCTTGAATTTGAAAATTTAGGGGTTTGTTTTGAACCTCAATTGGTGATTATCTATCATACTTTTAATTACTTATCTGGTTAGTTCTTGTTTTGAAATCTACAATAATAATAGTGGTAGATTTTCTTTACAA
TARGET=239,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8034
PRIMER_LEFT_PENALTY=0.193133
PRIMER_RIGHT_PENALTY=0.610296
PRIMER_LEFT_SEQUENCE=GTGTTCTNCTCAGCAGCCTATG
PRIMER_RIGHT_SEQUENCE=GAGGTTCAAAACAAACCCCTAA
PRIMER_LEFT=31,22
PRIMER_RIGHT=320,22
PRIMER_LEFT_TM=59.807
PRIMER_RIGHT_TM=59.390
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.50
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=290
PRIMER_PAIR_PENALTY_1=0.8128
PRIMER_LEFT_1_PENALTY=0.202525
PRIMER_RIGHT_1_PENALTY=0.610296
PRIMER_LEFT_1_SEQUENCE=GGCCAAGGAAGAAGAGAAGAAT
PRIMER_RIGHT_1_SEQUENCE=GAGGTTCAAAACAAACCCCTAA
PRIMER_LEFT_1=136,22
PRIMER_RIGHT_1=320,22
PRIMER_LEFT_1_TM=60.203
PRIMER_RIGHT_1_TM=59.390
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.8314
PRIMER_LEFT_2_PENALTY=0.221115
PRIMER_RIGHT_2_PENALTY=0.610296
PRIMER_LEFT_2_SEQUENCE=TATGCCGATTATGATGGATGAG
PRIMER_RIGHT_2_SEQUENCE=GAGGTTCAAAACAAACCCCTAA
PRIMER_LEFT_2=49,22
PRIMER_RIGHT_2=320,22
PRIMER_LEFT_2_TM=59.779
PRIMER_RIGHT_2_TM=59.390
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.8314
PRIMER_LEFT_3_PENALTY=0.221115
PRIMER_RIGHT_3_PENALTY=0.610296
PRIMER_LEFT_3_SEQUENCE=CTATGCCGATTATGATGGATGA
PRIMER_RIGHT_3_SEQUENCE=GAGGTTCAAAACAAACCCCTAA
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=320,22
PRIMER_LEFT_3_TM=59.779
PRIMER_RIGHT_3_TM=59.390
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.8343
PRIMER_LEFT_4_PENALTY=0.224014
PRIMER_RIGHT_4_PENALTY=0.610296
PRIMER_LEFT_4_SEQUENCE=GGAAGCGATGTTAAGATTCTCG
PRIMER_RIGHT_4_SEQUENCE=GAGGTTCAAAACAAACCCCTAA
PRIMER_LEFT_4=167,22
PRIMER_RIGHT_4=320,22
PRIMER_LEFT_4_TM=60.224
PRIMER_RIGHT_4_TM=59.390
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_04862 Ps_contig_mira-and-tgicl-ass_franssen_34882
SEQUENCE=TTGGTTTATTATTAATGATCAAGTATGTTATTCTATTTATTATACTAATTTTCTTGCCTTATTTCTTTTAAAACAAAATAGGAAACAAAAAAAAAATAAGCTAAGCTAAGCTTACATTTCCTGTTTTTCTCATAGCTTGTTTGTTATTATGCATCCAAACTTTGAAAACCTGTCTTTTGATACCAACTTGAGAACAAAACTGTTGAACTTCTTGTTCATCATGTTTCTGGATCTTCCACCCAATCCTTTCAGCAAACTCCATCATCTTATCCTTTTGAAGCTGTGA
TARGET=97,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04863 Ps_contig_mira-and-tgicl-ass_franssen_34887
SEQUENCE=GTAGGGAAATATNTTGTTGTCTTCTGGAGTNCTTAACCATTGGGCTGAAGTCCACTATGCAATTCTTCATGAAACGAAGTAATTTTAAGGGAACAATCTCATTTGGTATTGGGTTCTTGATTCTCATGGTGGGATGGCCTATTTTGGGCATGATTATTGAAGCTTACGGGTTCATCGTACTATTCAGTGGTTTCTGGCCTACAGTGTCTGTTTTTATACAGAAGATTCCTGTTCTTGGTTGGTTGGTTCAACTGCCATTCATCCGATCGTTATTTGACCGGAATAGGGGTAAACGAATGCCTGTGTAGTGTATCACATGGAAATCATTGCAAATATGTCACAACTAGCAAGCGCATTTGTAACCGAATGTAAAGAAAATACTCTCATATGGGGATCTAACAATGTGAACTGAGATCAACTTCTGATGGTAAGAAAATGATTCTATTTTTATCATGATAATCTAATTTTAAAGGACAAAAGAAGAAATTAACGAAGGAAGGGGTTTAAAAACGGG
TARGET=235,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4895
PRIMER_LEFT_PENALTY=0.186949
PRIMER_RIGHT_PENALTY=0.302565
PRIMER_LEFT_SEQUENCE=ATTGGGTTCTTGATTCTCATGG
PRIMER_RIGHT_SEQUENCE=ACCCCTATTCCGGTCAAATAAC
PRIMER_LEFT=107,22
PRIMER_RIGHT=289,22
PRIMER_LEFT_TM=60.187
PRIMER_RIGHT_TM=60.303
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.6039
PRIMER_LEFT_1_PENALTY=0.186949
PRIMER_RIGHT_1_PENALTY=0.416908
PRIMER_LEFT_1_SEQUENCE=ATTGGGTTCTTGATTCTCATGG
PRIMER_RIGHT_1_SEQUENCE=TACCCCTATTCCGGTCAAATAA
PRIMER_LEFT_1=107,22
PRIMER_RIGHT_1=290,22
PRIMER_LEFT_1_TM=60.187
PRIMER_RIGHT_1_TM=59.583
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.6039
PRIMER_LEFT_2_PENALTY=0.186949
PRIMER_RIGHT_2_PENALTY=0.416908
PRIMER_LEFT_2_SEQUENCE=ATTGGGTTCTTGATTCTCATGG
PRIMER_RIGHT_2_SEQUENCE=TTACCCCTATTCCGGTCAAATA
PRIMER_LEFT_2=107,22
PRIMER_RIGHT_2=291,22
PRIMER_LEFT_2_TM=60.187
PRIMER_RIGHT_2_TM=59.583
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.6243
PRIMER_LEFT_3_PENALTY=0.186949
PRIMER_RIGHT_3_PENALTY=0.437312
PRIMER_LEFT_3_SEQUENCE=ATTGGGTTCTTGATTCTCATGG
PRIMER_RIGHT_3_SEQUENCE=TTACAAATGCGCTTGCTAGTTG
PRIMER_LEFT_3=107,22
PRIMER_RIGHT_3=361,22
PRIMER_LEFT_3_TM=60.187
PRIMER_RIGHT_3_TM=60.437
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.7324
PRIMER_LEFT_4_PENALTY=0.186949
PRIMER_RIGHT_4_PENALTY=0.545451
PRIMER_LEFT_4_SEQUENCE=ATTGGGTTCTTGATTCTCATGG
PRIMER_RIGHT_4_SEQUENCE=AGGCATTCGTTTACCCCTATTC
PRIMER_LEFT_4=107,22
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=60.187
PRIMER_RIGHT_4_TM=60.545
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_04864 Ps_contig_mira-and-tgicl-ass_franssen_34889
SEQUENCE=CGGAAAGCGAAGTAACGCCAGAGAAGAAAAGAAAGTAGGAAAACATCTATCTTCCCTTTACATTACTACACAACTCTCTCTCTCATCACTGAATTATTCTTTTGCATCTTCAAGCTCCGTCAATGGCTTCTGGATCGACTGGTCGCGGTAACCCCGGTTCTAAAGGCTTTGATTTTGCTTCTGATGACATCTTATGCTCCTACGAAGACTTTTCCAACAGGGATTCCAATTCCAATGGCAATCACAACGATCCTGTAATCGCCTCTAATTCAACCACGGATTTTCACAAATCAAGGGTAGCAAGGGCTTCCGTTTTTACTGCTACTGCCTATAATCCACCTGAAGATTCATTAAACCAAGATGTGATTGCAACTGTGGAGAAGAGTATGAAGAAATATGCTGACAACCTCATGCGATTTCTTGAGGGAATTAGTTCAAGGCTATCACAGTTGGAGTTATATTGCTACAATCTTGACAAATCTATTGGAGAAATGAGATCTGATTTAAATCGTGACCATGGGGAGCAAGATTCAAAGCTTAAGTCTCTTGAAAAGCATCTTCAGGAAGTTCACCGGTCGGTACAAATTTTAAGAGACAAGCAAGGGTTAGCTGAGACTCAGAAAGAACTAGCCAAGCTTCAACTTGCTCAGAAGGAATCATCTTCCTCAAGCCATTCACAGTCCAATGAGGACAGATCCTCACCTTCTRCAGATACCTAAAAAAACTGAATAATGCATCTGATGCGAA
TARGET=74,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04865 Ps_contig_mira-and-tgicl-ass_franssen_34895
SEQUENCE=TACATTAGAAATCTTTCTCTCTTTATATTATAATAGAATAAAATGTCAACTACAGTCTACAAATAACAAAGACAATCCTTACTTTCACTGCAAAACCACATATAGATTACAAATATGTTTTTGAATACTTCCTAAAATGGCCGCTGGAACCCTCCTAAACTAAATAGGGAATGAAGGAAAGCTGATTCACTAATAAAAGGGATATTCGAAAATGTACCAAGAAGAGCACACCTGATGGAATGCATGGTTATGAGAATATATGCCAACGCCACACCAGCCCAGTAATAGATCCCAAGTGTTCCCTTGAAGTGTATGAGCGGCAAGAAATTGCTTGCAACCCATATTATCTGCAGAGCAATTTCCAGTAGCATTCCCGCCATTACATGGAATCTGAAGATAACGGGAAAGTCCCTATTTTTTACCACTACCACGATAGCCAAATAGCAGTATAATATAGGGAACCAGGTTGGCAATCGGTTGACAGCTCCTGGGATATAAAAGATCAGATTTTCAAAGAGCTTGTATTTATCAAGTAAAGGTTCTATGTAATACCCAGTTGCAGACATCTGCAATGCTATTAGATAGGGTATACAGGATAAAGTCCTCCACCACCATTCTGGCTTCTCAGCATTTGCAGGGATTCGAAAGCCAGACCAATGGTATATATATGCTTGTGTACGGGTGCTAGAACTGCCTCTTCGAGATATAGGAACACCGAGTGAGAGATGACCATGGTTATCAGTAGATAGTGGAGATGAAACAGCCGTGGGTCGCGTAGCTGATTTTCCCAGAGAACTGCCTGCAAGAGCTTTGATGGCAAAATTCCTTTTCCTGTAGAATGTAACTCGTCTTTCTCGAATCAAATCTTTATTCGAGAGAGAAGGAAGGCGGAGAAGAGAAACGTTGGTTGTTGTTGCTGCGGCGGCGGCGAATTGACCTGCCATCGTCTTCTTTCTACTTTCTTCTCGACTCCTCCGTTACCACCTTTTTATTATTATCCTTTTATTTTGTTTTTTATTTTATTCCCCGG
TARGET=919,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1266
PRIMER_LEFT_PENALTY=0.114470
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=AGCTGATTTTCCCAGAGAACTG
PRIMER_RIGHT_SEQUENCE=CGGAGGAGTCGAGAAGAAAGTA
PRIMER_LEFT=776,22
PRIMER_RIGHT=976,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=201
PRIMER_PAIR_PENALTY_1=0.2289
PRIMER_LEFT_1_PENALTY=0.114470
PRIMER_RIGHT_1_PENALTY=0.114470
PRIMER_LEFT_1_SEQUENCE=AGCTGATTTTCCCAGAGAACTG
PRIMER_RIGHT_1_SEQUENCE=GTAACGGAGGAGTCGAGAAGAA
PRIMER_LEFT_1=776,22
PRIMER_RIGHT_1=980,22
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.3765
PRIMER_LEFT_2_PENALTY=0.114470
PRIMER_RIGHT_2_PENALTY=0.261998
PRIMER_LEFT_2_SEQUENCE=AGCTGATTTTCCCAGAGAACTG
PRIMER_RIGHT_2_SEQUENCE=GTAGAAAGAAGACGATGGCAGG
PRIMER_LEFT_2=776,22
PRIMER_RIGHT_2=957,22
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=60.262
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=182
PRIMER_PAIR_PENALTY_3=0.3765
PRIMER_LEFT_3_PENALTY=0.114470
PRIMER_RIGHT_3_PENALTY=0.261998
PRIMER_LEFT_3_SEQUENCE=AGCTGATTTTCCCAGAGAACTG
PRIMER_RIGHT_3_SEQUENCE=TAGAAAGAAGACGATGGCAGGT
PRIMER_LEFT_3=776,22
PRIMER_RIGHT_3=956,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=60.262
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.3811
PRIMER_LEFT_4_PENALTY=0.114470
PRIMER_RIGHT_4_PENALTY=0.266650
PRIMER_LEFT_4_SEQUENCE=AGCTGATTTTCCCAGAGAACTG
PRIMER_RIGHT_4_SEQUENCE=AAAAAGGTGGTAACGGAGGAGT
PRIMER_LEFT_4=776,22
PRIMER_RIGHT_4=989,22
PRIMER_LEFT_4_TM=59.886
PRIMER_RIGHT_4_TM=60.267
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_04866 Ps_contig_mira-and-tgicl-ass_franssen_34908
SEQUENCE=CCTTCCCGACGTCGTATAGTTGTACCATGACATGCCTGTTTGTTATTTGGCTCATGTGCATCATCATYATCATCATCATCATCAGTACTCTGAGAATGGCTTAAATTTTCCTGAGGATGAGAAGGTAAAGCGGAGGCATTGAGTTCATCACCTTCTTCGCTGGGAAGTTGAGTAGGGAAAGCAGAGAGACGAAGAGGAGCCGCAACCAATGAGTGTGAATGTGAGGGTTGGGTGATTTTGAGGAAAGAAGGTCGAGTTGTTACTCCGCCGGCGGAGATGATGGAAGAAGGAGTGTGGTGGTGGTGATGGTGGAGGAGTGAGTGAGTGTCGGAGATCGAATGAAACGACATCGCTTCCATTGAATTGGGATATTATTCGATGATGGAAAATAGAGCGGTGAGGTTTTGAAGCTAAACCCCGTTGGCGTATTCAAAGAGAGAATATATACACTTTCTCTTCGCTGATAGGGATGAGAGTTAGGGCGTGACTCGGAT
TARGET=69,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04867 Ps_contig_mira-and-tgicl-ass_franssen_34908
SEQUENCE=CCTTCCCGACGTCGTATAGTTGTACCATGACATGCCTGTTTGTTATTTGGCTCATGTGCATCATCATYATCATCATCATCATCAGTACTCTGAGAATGGCTTAAATTTTCCTGAGGATGAGAAGGTAAAGCGGAGGCATTGAGTTCATCACCTTCTTCGCTGGGAAGTTGAGTAGGGAAAGCAGAGAGACGAAGAGGAGCCGCAACCAATGAGTGTGAATGTGAGGGTTGGGTGATTTTGAGGAAAGAAGGTCGAGTTGTTACTCCGCCGGCGGAGATGATGGAAGAAGGAGTGTGGTGGTGGTGATGGTGGAGGAGTGAGTGAGTGTCGGAGATCGAATGAAACGACATCGCTTCCATTGAATTGGGATATTATTCGATGATGGAAAATAGAGCGGTGAGGTTTTGAAGCTAAACCCCGTTGGCGTATTCAAAGAGAGAATATATACACTTTCTCTTCGCTGATAGGGATGAGAGTTAGGGCGTGACTCGGAT
TARGET=294,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04868 Ps_contig_mira-and-tgicl-ass_franssen_34908
SEQUENCE=CCTTCCCGACGTCGTATAGTTGTACCATGACATGCCTGTTTGTTATTTGGCTCATGTGCATCATCATYATCATCATCATCATCAGTACTCTGAGAATGGCTTAAATTTTCCTGAGGATGAGAAGGTAAAGCGGAGGCATTGAGTTCATCACCTTCTTCGCTGGGAAGTTGAGTAGGGAAAGCAGAGAGACGAAGAGGAGCCGCAACCAATGAGTGTGAATGTGAGGGTTGGGTGATTTTGAGGAAAGAAGGTCGAGTTGTTACTCCGCCGGCGGAGATGATGGAAGAAGGAGTGTGGTGGTGGTGATGGTGGAGGAGTGAGTGAGTGTCGGAGATCGAATGAAACGACATCGCTTCCATTGAATTGGGATATTATTCGATGATGGAAAATAGAGCGGTGAGGTTTTGAAGCTAAACCCCGTTGGCGTATTCAAAGAGAGAATATATACACTTTCTCTTCGCTGATAGGGATGAGAGTTAGGGCGTGACTCGGAT
TARGET=315,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04869 Ps_contig_mira-and-tgicl-ass_franssen_34910
SEQUENCE=ATAAAGAAACAACTACCAGACCACCTCGACCTCGCTTCGTGTTCCTCCGCCTATCATTTGGTCATGAATGAACCAGATAAAGAAAGAAATTTCAAATAAGTCCCTCCATATGTCAATGTGCCCCCAGAAGCATCGGGCTGACGAAGTGTGACACTTTTTATGACACCATTTGCTGAAAGAACACATAGAGCTCGAGGCCCTTGCTGAGAAAATGATATTACTTTCATAGTAACATCCTCTCCAGTATTTAACAGTGATGATGTGAGGTGTGAAATTTGAACTAGTTGAGCAAGGGACCCATTCACCTATATTTTCCAACTCGAATTTGCCTTTGCTCACCGAGCTAACCGGCCGCACCTTCGCACGCTTCTCCGCCGTGAAATCGATCACCGGTGGCAACGGAGCAGAGGAAGATATCGGCTTCGGAGACAGCGCTATCCTCACCGAACCGTCCGCTGCATACTTTCTCGGCCGGCCTCTCTTCTTCTTCTCAGGAACCGTCGTATCTGCCGGGACCACCGTTACCACCGCCGCATGCGGCGGATTCTGCGGCTGAGAATTTCCTTGGACCGCTGTAGTAAACTGAACTACNGATCCACCGGTCGGAGTTTGGTTCTCGGTTCTCGGTGCTACATGGAACTCTGACGGAGCATCCGATCCCACCACCGTAACGCCACCACTAACA
TARGET=480,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0604
PRIMER_LEFT_PENALTY=1.039247
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=GAGCAGAGGAAGATATCGGCT
PRIMER_RIGHT_SEQUENCE=TACTACAGCGGTCCAAGGAAAT
PRIMER_LEFT=401,21
PRIMER_RIGHT=579,22
PRIMER_LEFT_TM=59.961
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=1.2326
PRIMER_LEFT_1_PENALTY=1.039247
PRIMER_RIGHT_1_PENALTY=0.193337
PRIMER_LEFT_1_SEQUENCE=GAGCAGAGGAAGATATCGGCT
PRIMER_RIGHT_1_SEQUENCE=TCAGTTTACTACAGCGGTCCAA
PRIMER_LEFT_1=401,21
PRIMER_RIGHT_1=585,22
PRIMER_LEFT_1_TM=59.961
PRIMER_RIGHT_1_TM=59.807
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=1.2326
PRIMER_LEFT_2_PENALTY=1.039247
PRIMER_RIGHT_2_PENALTY=0.193337
PRIMER_LEFT_2_SEQUENCE=GAGCAGAGGAAGATATCGGCT
PRIMER_RIGHT_2_SEQUENCE=TTCAGTTTACTACAGCGGTCCA
PRIMER_LEFT_2=401,21
PRIMER_RIGHT_2=586,22
PRIMER_LEFT_2_TM=59.961
PRIMER_RIGHT_2_TM=59.807
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=1.3279
PRIMER_LEFT_3_PENALTY=1.039247
PRIMER_RIGHT_3_PENALTY=0.288644
PRIMER_LEFT_3_SEQUENCE=GAGCAGAGGAAGATATCGGCT
PRIMER_RIGHT_3_SEQUENCE=AGTTTACTACAGCGGTCCAAGG
PRIMER_LEFT_3=401,21
PRIMER_RIGHT_3=583,22
PRIMER_LEFT_3_TM=59.961
PRIMER_RIGHT_3_TM=59.711
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=1.3296
PRIMER_LEFT_4_PENALTY=1.308434
PRIMER_RIGHT_4_PENALTY=0.021195
PRIMER_LEFT_4_SEQUENCE=CAAGGGACCCATTCACCTATATT
PRIMER_RIGHT_4_SEQUENCE=TACTACAGCGGTCCAAGGAAAT
PRIMER_LEFT_4=289,23
PRIMER_RIGHT_4=579,22
PRIMER_LEFT_4_TM=60.308
PRIMER_RIGHT_4_TM=60.021
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_04870 Ps_contig_mira-and-tgicl-ass_franssen_34929
SEQUENCE=CAARGRRGRAGGGTCCACCACAACCATAACACAACCAATTGGAGCCTCTTACTCTTCAATGAGTTGCAAGTACATTTTCACCTTTCAAATACCTCTCCTTAATTGTTGAATTATCTTCTTGAGTTCATTCTCAGCCATGGCTAATACTGATAGTCTCAGTAATCTTGATATAAACAGTATCTGTGATGATGGCTCTGTCAGGAATTTTTCTGCTAACTGGCCAATGTCAACATCTGGACAGAATGCAAAAGGTCAATTTGCCATTCTCTATAATGGAAGCATGTGTGTATATGATGGAATTCCTAGAGAAAAGGTTGAAGAAATATTGATGATGGCTGCTGCCACTGCCAAGTCTTCTGAAATGAAAAGTGGAATTCCATTCACTTCACTGTTTACCAGTTTTGCCACACCTTCTTCTCCACAAGGAACTTCTAACAATGTGCCTTCTCCTCCATCAGTCTGTTTTCCTGCTGCTGACAAGAGTTCCATTTGCAGGATGCAAGAATTTCCATTAGCACGCAGACAGTCACTTCAAAGTTTTCTTGAGAAGAGAAGGATGAGGGTACGTGGCAAAGTACCTTATACCTCTTCATCATCAAAAGGAACTAACAACATTGACAACAACTTCAACACAGCATTGGTTCCTTCCACTTGAAGACAGATATGTTATGTTATGTTTTCTACACAATATTATAAGCCTAATTAATTTATTATTATTATTATTATTATTATTATTGTTTTTGAACCTTAATGTGTATGTTATACTATGCTTAAAGTGTTGTTAATGTGTCTGAACAATTGATTATCAATTATGTGTTATTTCCTTTGTAAYACNAAAA
TARGET=663,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04871 Ps_contig_mira-and-tgicl-ass_franssen_34929
SEQUENCE=CAARGRRGRAGGGTCCACCACAACCATAACACAACCAATTGGAGCCTCTTACTCTTCAATGAGTTGCAAGTACATTTTCACCTTTCAAATACCTCTCCTTAATTGTTGAATTATCTTCTTGAGTTCATTCTCAGCCATGGCTAATACTGATAGTCTCAGTAATCTTGATATAAACAGTATCTGTGATGATGGCTCTGTCAGGAATTTTTCTGCTAACTGGCCAATGTCAACATCTGGACAGAATGCAAAAGGTCAATTTGCCATTCTCTATAATGGAAGCATGTGTGTATATGATGGAATTCCTAGAGAAAAGGTTGAAGAAATATTGATGATGGCTGCTGCCACTGCCAAGTCTTCTGAAATGAAAAGTGGAATTCCATTCACTTCACTGTTTACCAGTTTTGCCACACCTTCTTCTCCACAAGGAACTTCTAACAATGTGCCTTCTCCTCCATCAGTCTGTTTTCCTGCTGCTGACAAGAGTTCCATTTGCAGGATGCAAGAATTTCCATTAGCACGCAGACAGTCACTTCAAAGTTTTCTTGAGAAGAGAAGGATGAGGGTACGTGGCAAAGTACCTTATACCTCTTCATCATCAAAAGGAACTAACAACATTGACAACAACTTCAACACAGCATTGGTTCCTTCCACTTGAAGACAGATATGTTATGTTATGTTTTCTACACAATATTATAAGCCTAATTAATTTATTATTATTATTATTATTATTATTATTGTTTTTGAACCTTAATGTGTATGTTATACTATGCTTAAAGTGTTGTTAATGTGTCTGAACAATTGATTATCAATTATGTGTTATTTCCTTTGTAAYACNAAAA
TARGET=708,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04872 Ps_contig_mira-and-tgicl-ass_franssen_34930
SEQUENCE=CTTGGTGGGGGTGCAATGCTTGATACACGGGGAGTAGATGGGGAAGTTCTGCATGTTAATTTTCCTTCAGAAGAGGAGGCATCAACACTGGTATCTACCAGTCAGAAGCAATACAAGGAACGTTTGGAAAAAGCAATTCAAATTCCTGATGTAGAAGATGAAGTTTCTGGACCCAAGGGTATGGAGCTTTCAAGAACTCCCATTGAACTCAAAAGTAGTGAGTGGCTTCTTAAAGTTGTTCCCTGGATTGGGGGTAGAATCATCTCCATGATACACTTTCCTTCAGGAACACAATGGCTCCATGGTAGGATTGAAATCAGTGGATATGAGGAGTACAGTGGTACAGAATATCGTTCTGCTGGGTGTTCTGAGGAATATTCTATAATCAATCGAGAACTTGGGCATGCTGGAGAGGAGGAATCTGTGGTATTAGAGGGTGATATTGGTGGAGGATTGGTTCTTCAACGGAAGATAAATTTTCCAAAGAATGCTGCTAACATCATCCAAATCAACTCTAGCATAATAGCGCGCAGCGTCGGAGCTGGTTCTGGAGGATTCTCAAGGTTGGTCTGCTTAAGAATTCACCCAACATTCAATCTTTTGCACCCAACAGAATCATTTGTCTCATTTACTTCAATTGATGGATCGGTGCATGAAGTCTTTCCAGATGGTGGGGAGCAAATCTTCGAAGGAAACCTTATTCCTGACGGTGAGTGGAGGCTCGTTGATAAATGTCTTGGCTTGGCACTGGTCAATCGATTTAATGTTACAGAGATTTTGAAGTGTCTTGTTCACTGGGATTTTGGCACTGTCAACTTGGAGCTTTGGTCGGAGAGCAGACCTGTCTCAGAGCATTCTCCTATTCAGATTTCTCATCAGTATGAAGTTATACGAATTCCTTAGTGTTTTCCGTTGGTCACTACACAAGGTTTCTGACTGTTTGAAAGGGCAGAGTGGTGCTACTAGAGATTATTAAGTATACAAAAACTATGTATCTAAATTCAAGCAGGCTCTCCATTCGCAATGGGTATTTGGTTGGACTTTGATCAATAAATAAAATGAATGGAGTTATGGAGGAGGAATGGTAATAGCATAATGTGTTGAGAAAGATAGCACTAAACACACCTAATACATTAGGTCACTGGAATAAATTAATTACTCCCCTGGAATACCTAAAACCTTTTTCCCCAACTTAATTAAATAAATAAA
TARGET=1198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_04873 Ps_contig_mira-and-tgicl-ass_franssen_34943
SEQUENCE=CAACAGTTTTATTCAGCTGATGAACTTTTCTTTTGAAGGCAAAATCCTTCCTTTTTGGCAGATGCAACATCTAGAGAAGCTCAACAAGATCAACCTGAAAGCGAAAGACGTTGAAGTAGAATTAGAAGGAGAAGAAGAAGAAGGAGAACATGAACATGAAGAGGAAGTGATTGAAGTTGTAGTCAACAACAAAGAAGATAGTAGTAGAGTAAATTGGTTTGTTGATGATGATCCATCACCAAGGCCACCAAAGTGCACTGTTCTATGGAAAGAGTTATTAAGGTTGAAGAAACAAAGAGCTTCATCTTTATCGCCTTCCTCGTCTTCATCGTCTTCGTCTTCGAACGCTAGCTCGCTCGGCGACGCGGCTGCTAAAGAAGGCTCGAGAAACAAAGAGAATCAACATGTGAAGAGGATAAAGAAAGGGTTGGAGAGAACAAGATCAGCTACTATTAGAATTAGACCAATGATTAATGTTCCAATTTGTACACAAATGAAGAACAGTGCTTTGCCTCCACTATTTCCACTTAAGAAGGGTAAATTAGAGAGGTAAT
TARGET=130,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4900
PRIMER_LEFT_PENALTY=0.107763
PRIMER_RIGHT_PENALTY=0.382228
PRIMER_LEFT_SEQUENCE=AAGATCAACCTGAAAGCGAAAG
PRIMER_RIGHT_SEQUENCE=GAAGACGATGAAGACGAGGAAG
PRIMER_LEFT=85,22
PRIMER_RIGHT=335,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=60.382
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.6184
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.510616
PRIMER_LEFT_1_SEQUENCE=AAGATCAACCTGAAAGCGAAAG
PRIMER_RIGHT_1_SEQUENCE=GAAGGCGATAAAGATGAAGCTC
PRIMER_LEFT_1=85,22
PRIMER_RIGHT_1=317,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=59.489
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.6313
PRIMER_LEFT_2_PENALTY=0.249074
PRIMER_RIGHT_2_PENALTY=0.382228
PRIMER_LEFT_2_SEQUENCE=GATCAACCTGAAAGCGAAAGAC
PRIMER_RIGHT_2_SEQUENCE=GAAGACGATGAAGACGAGGAAG
PRIMER_LEFT_2=87,22
PRIMER_RIGHT_2=335,22
PRIMER_LEFT_2_TM=60.249
PRIMER_RIGHT_2_TM=60.382
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.6865
PRIMER_LEFT_3_PENALTY=0.107763
PRIMER_RIGHT_3_PENALTY=0.578731
PRIMER_LEFT_3_SEQUENCE=AAGATCAACCTGAAAGCGAAAG
PRIMER_RIGHT_3_SEQUENCE=AGACGAGGAAGGCGATAAAGAT
PRIMER_LEFT_3=85,22
PRIMER_RIGHT_3=324,22
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=60.579
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=0.7532
PRIMER_LEFT_4_PENALTY=0.107763
PRIMER_RIGHT_4_PENALTY=0.645442
PRIMER_LEFT_4_SEQUENCE=AAGATCAACCTGAAAGCGAAAG
PRIMER_RIGHT_4_SEQUENCE=GAGGAAGGCGATAAAGATGAAG
PRIMER_LEFT_4=85,22
PRIMER_RIGHT_4=320,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=59.355
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_04874 Ps_contig_mira-and-tgicl-ass_franssen_34946
SEQUENCE=CTTAATCTCTCTGCAAATCTTTTGGAATCACTGCCCGATTCAATTGGGTTGCTACAGAAGTTGAAATTGCTCAATGTTTCTGGAAACAAGCTTGCAGCCCTTCCTGATTCCATTTGTCAGTGCAGGTCATTGGTGGAGCTGGATGCAAGCTTTAACAATCTGTCATACTTGCCAACAAACATTGGGTATGAATTACCGAACTTGAAGAAACTCATGATTCATTTGAACAAAATCCGATCTCTTCCCTCGTCCATTTGTGAGTTGAAGTCGTTACGCTATTTGGATGCTCACTTCAATGAGCTACATGGGCTTCCAATAGCAATTGGGAGACTGACTAATCTGGAAGTTCTCAACTTGAGCAGTAACTTCACTGATCTTAAAGAACTTCCAGAGACGTTTGGTGAATTGACTAATCTCAGGGAATTGGATCTCAGCAATAATCAGATTCATGCACTTCCCGATACATTCGGTTACCTTGATAATTTGACCAAGCTCAACTTGGAACAGAATCCTCTTGAACTGCCACCAGTGGAGATTGTAAACCAAGGGGTCGAAGCCATATTGACTTTTATGACCAAGAGGTGGATTGATATCTTGCAAGAGGAAGAAAGAAAGAGCAATCAAGAAATGCAAGAACAGGTAGAGGGAGGCTGGTTAACACGAAGCACGTCGTGGTTGAAGAATGTTTCTGGAAATGTAGCTGGGTATATTGGAACTGCTGTTGGATCTCCTATGTCACCCAAATCTCCCGAGGAAGCTTATCTTAATCAACAGCTATAAGGTGAGATTTGTCCGACTCTTTATCAGGAATTGTCAAGCATGCATTTTTGATGAAGATTACAGATTACAGGGTCTATGTTTGTTATGTTTGTTACCAAGCAGCAGTTCCTGCTGTTACTTCCTCTTTTGTTCTCAACCCAATATCCAACATAAGCGTAGGCATGGTGCTTTGGTAATTAACGTGTATAATCGAGGGGCATGAATGCCACTCAACAATAACTGACCTTAAATTTTGATTAATTTTGTTTTAAACTTTAAAAAAAATTAAAAACAAAATTAA
TARGET=605,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0916
PRIMER_LEFT_PENALTY=0.069526
PRIMER_RIGHT_PENALTY=0.022096
PRIMER_LEFT_SEQUENCE=AGGGAATTGGATCTCAGCAATA
PRIMER_RIGHT_SEQUENCE=GTTAACCAGCCTCCCTCTACCT
PRIMER_LEFT=417,22
PRIMER_RIGHT=658,22
PRIMER_LEFT_TM=59.930
PRIMER_RIGHT_TM=60.022
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.1308
PRIMER_LEFT_1_PENALTY=0.108684
PRIMER_RIGHT_1_PENALTY=0.022096
PRIMER_LEFT_1_SEQUENCE=CACCAGTGGAGATTGTAAACCA
PRIMER_RIGHT_1_SEQUENCE=GTTAACCAGCCTCCCTCTACCT
PRIMER_LEFT_1=523,22
PRIMER_RIGHT_1=658,22
PRIMER_LEFT_1_TM=59.891
PRIMER_RIGHT_1_TM=60.022
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.1999
PRIMER_LEFT_2_PENALTY=0.069526
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=AGGGAATTGGATCTCAGCAATA
PRIMER_RIGHT_2_SEQUENCE=TCCCTCTACCTGTTCTTGCATT
PRIMER_LEFT_2=417,22
PRIMER_RIGHT_2=647,22
PRIMER_LEFT_2_TM=59.930
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.2161
PRIMER_LEFT_3_PENALTY=0.069526
PRIMER_RIGHT_3_PENALTY=0.146525
PRIMER_LEFT_3_SEQUENCE=AGGGAATTGGATCTCAGCAATA
PRIMER_RIGHT_3_SEQUENCE=CAGAAACATTCTTCAACCACGA
PRIMER_LEFT_3=417,22
PRIMER_RIGHT_3=690,22
PRIMER_LEFT_3_TM=59.930
PRIMER_RIGHT_3_TM=60.147
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.2390
PRIMER_LEFT_4_PENALTY=0.108684
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=CACCAGTGGAGATTGTAAACCA
PRIMER_RIGHT_4_SEQUENCE=TCCCTCTACCTGTTCTTGCATT
PRIMER_LEFT_4=523,22
PRIMER_RIGHT_4=647,22
PRIMER_LEFT_4_TM=59.891
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_04875 Ps_contig_mira-and-tgicl-ass_franssen_34947
SEQUENCE=TCGGGGTTACGTTTTTATTTTTCTTATTCTTTCTTTGGTTCTAATTCAAATCCGTAGAATATCTCTGAATTTCTCTTCTCTGCCTAGGGTTCCAACCCAACTCTCTTCCAAGGGAAAATGAAAGCTATTTTCTGTGGAAATTTTGAATATGATTGCCGGGAATCTGAGCTGGAGCGGCTTTTTAGACGATATGGCAAGGTCGATAGGGTGGATATGAAAGCTGGATTTGCTTTTATTTATATGGAAGATGAGAGAGATGCTGATGCTGCAATTCGTGCTCTCGATCGGATAGAATTTGGTCGGAAGGGGCGTAGACTCCGTGTAGAATGGACAAAGCAAGAACGTGGCATTCGAAGGCCTGCTGAGCGCCCAAAAAGATCTTCAGCTAATGCGAGACCATCAAAGACCCTATTTGTAATTAATTTTGATACAAACCATACCAGAACAAGGGACTTGGAGAGGCACTTTGAGCCATATGGTAAGATAGTCAGTGTGAGGATCAGGAGGAATTTTGCATTTGTTCAGTATGAATCAGAAGATGATGCTTCCAAAGCCCTGGAAGCTACAAATATGAGCAAGTTGTTGGATCGGGTTATTTCAGTTGAATTTGCTGCCAAAGACGACGATGTCAGGCGAAATGGACATAGCCCGGAGAGAGGCCGTGATCGTCAGCGTGACAGAAGCCTTGATGGAAGGCGATCGCCCAGTCCTTATCGTAGAGAAAGGGGTAGTCCTGATTACGGCCATGGGTCTAGTCCATATAAGAGGCAGAGGAGCAGCCCTGATTATGGGCATGGCAACAGCCGTAGTAGAAGCCCCTATCGAAGAGAGCGAGGTAGCCCTGTTTACGGGCGTCGAAGCATTAGTCCCTACAGAAGAGAGAGGGATGATTCTGTGCCTGTTCATGTCAACAGCCGCAGTCCTAATCACAAAGAGCGGGGGAGAACCAACCGGTCTCCCTCCCATAGTCTCGAAGAAGGAGAGACTCAAAAAGGTCATGGATCTGACCTTAGTCCAGAAGAAGGGGAGAGGATAGACCCTAAAAAAGGTCCTAGGTTTGGCCATAGTCCCGAAGAGGGAGAGAGGATAGATCCTAGAAAAGGTCATGAATCTGACCATAGTCCTTATGTTGCTGTGAAGGGTAGCCCTGAAAATGGCCATTACCAGAGGCACAGTCCTGACACAATACGGAACCCCAGCCCTTACAAGGATTCTAGAGGGAGTCCTAGGCCTGATGCAAAAGGGAACCCCGGCCCATGCAATGATTATGGAGGGAGCCCAAATACTATGCCTGAACCCAGAGACAGTCCCAACTATGGTGGTCCTGAAAGTCCCATGCATGAACAAAGCCAGAGCCAGTCACCCCCTGCAGAAGAATGATTTCAGTTGCAATGAAAAAAGCCTGAAGGGCCAACAAATTGAATAACGTGGTCGTTACAGGACTAAATTTAGTTTTCAGCAAATGGCTTTAGTGTAGTGTAGTGTTGATAAAGCTTTGAAACTTTGGTGGTTTGACACTTTTCCTGTCATTTATTCATTTAGATAGTTTCATACTTCAATCTCAGTACAATGTTGTAGAATTTTTGTTTAAAGTCAAATAGTGGTGGCACCTATATTTTTGATGAATAGCATGAAACTTATGTTGTCTTATAAAAGTTGTTTTGACGNAACC
TARGET=1470,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1175
PRIMER_LEFT_PENALTY=0.031122
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=AACCCAGAGACAGTCCCAACTA
PRIMER_RIGHT_SEQUENCE=GTGTCAAACCACCAAAGTTTCA
PRIMER_LEFT=1294,22
PRIMER_RIGHT=1517,22
PRIMER_LEFT_TM=60.031
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.1520
PRIMER_LEFT_1_PENALTY=0.080323
PRIMER_RIGHT_1_PENALTY=0.071684
PRIMER_LEFT_1_SEQUENCE=TGAATAACGTGGTCGTTACAGG
PRIMER_RIGHT_1_SEQUENCE=AGGTGCCACCACTATTTGACTT
PRIMER_LEFT_1=1419,22
PRIMER_RIGHT_1=1611,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.928
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.2633
PRIMER_LEFT_2_PENALTY=0.176967
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=TATGGAGGGAGCCCAAATACTA
PRIMER_RIGHT_2_SEQUENCE=GTGTCAAACCACCAAAGTTTCA
PRIMER_LEFT_2=1266,22
PRIMER_RIGHT_2=1517,22
PRIMER_LEFT_2_TM=59.823
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=4.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=0.3330
PRIMER_LEFT_3_PENALTY=0.031122
PRIMER_RIGHT_3_PENALTY=0.301926
PRIMER_LEFT_3_SEQUENCE=AACCCAGAGACAGTCCCAACTA
PRIMER_RIGHT_3_SEQUENCE=ACAGGAAAAGTGTCAAACCACC
PRIMER_LEFT_3=1294,22
PRIMER_RIGHT_3=1526,22
PRIMER_LEFT_3_TM=60.031
PRIMER_RIGHT_3_TM=60.302
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.3627
PRIMER_LEFT_4_PENALTY=0.080323
PRIMER_RIGHT_4_PENALTY=0.282356
PRIMER_LEFT_4_SEQUENCE=TGAATAACGTGGTCGTTACAGG
PRIMER_RIGHT_4_SEQUENCE=GGTGCCACCACTATTTGACTTT
PRIMER_LEFT_4=1419,22
PRIMER_RIGHT_4=1610,22
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=60.282
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_04876 Ps_contig_mira-and-tgicl-ass_franssen_34953
SEQUENCE=CTTTTTTTATTGTTTAGTCTTACAGATACAATTAAAATTTTACTAAGTAAATAACTAACTTAACCAACCTAACAATGTCTATTTTTTGCTGAAGTTTTCAAGTGCTTGATTACAATCAGCTAATGAATGATTAAAGAAAAAACAGATCAAAAAAATACAAGTGTAGAGAGATATACAGATTACATATCATAACTCTTTGTCGATCGCAGCTTCCAAGTTTCAATGGAGTTACACTTTGGGAGGCAAACTTATAAGCTACAGATTCATGGATGGAGGTATTACGATTATCAAACAGATTCTGCTGGTTTATCCTTGGGAGCAGAGGACGATTCAAGAACCAAATCGCCGATTCCCTTGGGAGGACCGATGCTGCATCGAGCTGTGTTATGTGTCAAGATGGCATCCTTTATTTTTTGAAACTTGGCAAGTGAACATGAAAATGATTCCAGCTGCCCCTCAGCCCCTCTATAGAAGTGGAAGTAAGGAAGTACTTTAACATTCAGACTTTTGCACATTGGCTTATTCTCGTCAAAATTTACTTTCAAAAAAACAATCTCAGGGTGTTCTTCAGCTGTTCTACATAGCTTGGGGAATAATGCTCGGCAAGAAGCACACCAAGTCCCATAAAACTCAACAATAACTAGTCTATCCTCAGCTTGACTCAAAGCATTCAGAAACTCTTGTGTGGAGTGAATGTCAATCATGTTTGGTGCATTCTTTTCCCACCATTTTGGTGGACCATTTTCTGTAACAGCTGCACATACCTTGAAAGGAAGCAATTGTTTTCTGGATTTGAAATGCGACGGCGGTGGCAGAGAGAAAAGGCTCTTAGGTGGAAGCGGCGGGAGAGTAGTGAAGAAAGAGGGAGATGGAAGTCGAAGAGATTGAAATTGTAATCGAATAGTATGAACATGTGCCATTTATACACAAGACAAGTAAAGAGAGAAATAGTGATATACTAATAGTATGCTATGCTAGGAAGAGGAACAAGATTGAATTGGATAGAACATGAGTTGAGTTGTGATGAGATGAGATGAGAGAAGAAGTGTGTTTGAATTGAGTGGATAGGTTGAGTTTGAGGAGAAAAGAAAGGAAGCAAAATAAGATAGATTTTTTCTCTTTTTCTTCT
TARGET=1023,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0972
PRIMER_LEFT_PENALTY=0.622772
PRIMER_RIGHT_PENALTY=0.474473
PRIMER_LEFT_SEQUENCE=TGGCAGAGAGAAAAGGCTCTTA
PRIMER_RIGHT_SEQUENCE=TGCTTCCTTTCTTTTCTCCTCA
PRIMER_LEFT=809,22
PRIMER_RIGHT=1097,22
PRIMER_LEFT_TM=60.623
PRIMER_RIGHT_TM=60.474
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=1.1352
PRIMER_LEFT_1_PENALTY=0.018835
PRIMER_RIGHT_1_PENALTY=1.116415
PRIMER_LEFT_1_SEQUENCE=GCGGGAGAGTAGTGAAGAAAGA
PRIMER_RIGHT_1_SEQUENCE=TCTCCTCAAACTCAACCTATCCA
PRIMER_LEFT_1=841,22
PRIMER_RIGHT_1=1083,23
PRIMER_LEFT_1_TM=60.019
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=1.1864
PRIMER_LEFT_2_PENALTY=0.711929
PRIMER_RIGHT_2_PENALTY=0.474473
PRIMER_LEFT_2_SEQUENCE=GAAAAGGCTCTTAGGTGGAAGC
PRIMER_RIGHT_2_SEQUENCE=TGCTTCCTTTCTTTTCTCCTCA
PRIMER_LEFT_2=818,22
PRIMER_RIGHT_2=1097,22
PRIMER_LEFT_2_TM=60.712
PRIMER_RIGHT_2_TM=60.474
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=1.3401
PRIMER_LEFT_3_PENALTY=0.865663
PRIMER_RIGHT_3_PENALTY=0.474473
PRIMER_LEFT_3_SEQUENCE=TGCTATGCTAGGAAGAGGAACA
PRIMER_RIGHT_3_SEQUENCE=TGCTTCCTTTCTTTTCTCCTCA
PRIMER_LEFT_3=967,22
PRIMER_RIGHT_3=1097,22
PRIMER_LEFT_3_TM=59.134
PRIMER_RIGHT_3_TM=60.474
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=1.4218
PRIMER_LEFT_4_PENALTY=0.947336
PRIMER_RIGHT_4_PENALTY=0.474473
PRIMER_LEFT_4_SEQUENCE=GGGAGATGGAAGTCGAAGAGAT
PRIMER_RIGHT_4_SEQUENCE=TGCTTCCTTTCTTTTCTCCTCA
PRIMER_LEFT_4=863,22
PRIMER_RIGHT_4=1097,22
PRIMER_LEFT_4_TM=60.947
PRIMER_RIGHT_4_TM=60.474
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_04877 Ps_contig_mira-and-tgicl-ass_franssen_34986
SEQUENCE=GACATAGTAACAAATTACCTATAACTAATAGGGTTTCGTAAAAAAGAACAACAACAACTAGCAGATTTATTTAAAAACAAACTAATTAGCAAAACCGAATCCAATTAACAAGRCCAAGTAATCAAAGCAGTTGATGAAGGAAGTTTATCGTTCAAACAAGAATGTTTAGTAGTCAAATCCAAAACAGAAAAACTCGCTTCACTTCTCCGACAAGCAGCAACGTCAAGCTCCGTTTTAAACGAACGACCAGCACGTCGCATCATCGGAGACACCGTACAAGTCCTCGAAAAAGCACTAACGTTAGTCACCAAATGCAAAGTCAACGGTATGAAGCGCGTTTTCAGCATAGTACCTTCCGCCGCTTTCAGAAAAATGTCTTGTCGTCTTGAAAGTTCAATCGGCGATGTTTCCTGGCTACTCCGTGTCTCCGCTCCGACGGAGGAAGGTAGCTATGAGTGTCTAGGTCTACCACCTGTCGCTTCTAATGATCCGATTCTAGGTCTAATTTGGGAACAGATTGCGATTCTTCATAATGGCTCTGTTGATGATCGATCTGATGCTGCTGCTTCTTTGGTTTCGCTTGTTCGCGATAACGATCATTACGGAAAATTGATAATTGAAGAAGGTGGTGTTGGTCCTTTGTTGAAACTTATTAGAGAAGGAAAAGTAGAAGGTCAAGAAAATGCTGTTAAAGCTATTGGGTTTTTGGGTCGAGATGCAGAAAGT
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04878 Ps_contig_mira-and-tgicl-ass_franssen_34995
SEQUENCE=ACTTTTTCTAAAATTTAAATAATAAACTACAATGAAGAAATAAAATTNNAGANTTTNCCTAAAACTAGCTTAGGTTTACAACTGAGATTACCTTATTACTATTTGTTTAGCTTCTGCTATTTTTTTTCCTATCTTTCATATAAGCAACTATACTAGAATGAATATTATGCTAAGTACAACATTTGTCTTTCCCATTAAATTTGTACATAGCATCTCATTCAATTGCCTCAACGGAAAGTTGCACTGCGATTCCCAGAAGCAGGAAATGCTTGACGCCTTCTACCTTGTTGAAATTGTTTAGGCTCACTCGATTGTGCCTCTCCTATTGTCGAACGCTTCTTCTTGCCACCAGAGCCCTCTTTTTCCACACGATTGTCACAGTTCTGGGTAGATGGGTCTTTCTTTCTTTCTCCAAACTTAACATGGTTCATTGCTGCTTCGTAGTCAAATGGTGTCAGCTGCAATAAGCCACTTGCTTGTTCAGTTTTCTTTGGCTTTCTGGTTTTGTTATTTTGATTGTTTGCCTGAAAGCACTTTTGAAAGTTTGATGACAGTTCCGAGAGAGACCTTGGCTCGTCCTCATCCGAAGTTGAGGCACTAGAATTTTTCTCTGTGTGATCATTGGAGTTTTCTAAATTATTATCCTGCTCTATATCCTCCTCTGCACCTGTACCAGTGTCTAGCATTATAATCTCTTCTGGCGCAGAGGTGAAAACTGGATCAGAAACAGGTTTTTGTTGGTCTGGTGCTACAGTAAGAGTTTCTACTACAGCTTTTGAGGTCTCACTGCTACCCAAAAATGAATGGAAAGGTAGAGTGACTGAAGATCTAATTTGCTCTAGCTTGATCTCTTCTTTGCCCTTTTTATCAAGACCAAGCTTCCTCTTTGAAGTGGAGCTCCCCAGAAGTGACCCCG
TARGET=397,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0258
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.010545
PRIMER_LEFT_SEQUENCE=TCTTTTTCCACACGATTGTCAC
PRIMER_RIGHT_SEQUENCE=TCTCTCTCGGAACTGTCATCAA
PRIMER_LEFT=357,22
PRIMER_RIGHT=565,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.989
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.0816
PRIMER_LEFT_1_PENALTY=0.015253
PRIMER_RIGHT_1_PENALTY=0.066394
PRIMER_LEFT_1_SEQUENCE=TCTTTTTCCACACGATTGTCAC
PRIMER_RIGHT_1_SEQUENCE=AGCCAAAGAAAACTGAACAAGC
PRIMER_LEFT_1=357,22
PRIMER_RIGHT_1=495,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=139
PRIMER_PAIR_PENALTY_2=0.0816
PRIMER_LEFT_2_PENALTY=0.015253
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=TCTTTTTCCACACGATTGTCAC
PRIMER_RIGHT_2_SEQUENCE=ACTTTCAAAAGTGCTTTCAGGC
PRIMER_LEFT_2=357,22
PRIMER_RIGHT_2=543,22
PRIMER_LEFT_2_TM=60.015
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=0.0944
PRIMER_LEFT_3_PENALTY=0.028056
PRIMER_RIGHT_3_PENALTY=0.066394
PRIMER_LEFT_3_SEQUENCE=TGTCACAGTTCTGGGTAGATGG
PRIMER_RIGHT_3_SEQUENCE=AGCCAAAGAAAACTGAACAAGC
PRIMER_LEFT_3=373,22
PRIMER_RIGHT_3=495,22
PRIMER_LEFT_3_TM=60.028
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=123
PRIMER_PAIR_PENALTY_4=0.0944
PRIMER_LEFT_4_PENALTY=0.028056
PRIMER_RIGHT_4_PENALTY=0.066394
PRIMER_LEFT_4_SEQUENCE=TGTCACAGTTCTGGGTAGATGG
PRIMER_RIGHT_4_SEQUENCE=ACTTTCAAAAGTGCTTTCAGGC
PRIMER_LEFT_4=373,22
PRIMER_RIGHT_4=543,22
PRIMER_LEFT_4_TM=60.028
PRIMER_RIGHT_4_TM=59.934
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_04879 Ps_contig_mira-and-tgicl-ass_franssen_35002
SEQUENCE=AAATTCAACATTTTTTTGGTCTCATCAGCTTCTATATTGTTCACTCTCAATTTCGTCAATTGGGTTAGGTTTTTAGGCTTCTACTTGAGAGGCTTCATACCCTTTGATGCCCGCAGATCATTTACTTCCTTTCTAAGTGAATCATCAGAAAGGATTAACCGAGACACTGCTCCAGTAGATAACCCGAGATATTTTGCAGCATCTGAGACGGATCCGTCAACTGCAAAAATGAGATCCAGAAGAGCTTGCATTCCCATTGCAAATTTTGGGTTATTGGGTCCAATTTGTGAACCAATCTCTGCCCCTCTAATTAATGACTTGGGAGGGAGTATCTGAAGAAGCTCTCGAGGTGGTGCATAAGCATCAAGATCCACTGTTTTCCTGACTTTGAGAGCTATAAGAGAGCGAAGGCGTTTGAGAGCAGAAGCGCGGTTCATATGCTGAGAGCGATCTTCAGAAGCCTGAGCGATGATTCCAGTGGGTAAATGTTTGAGTCGAACAGCAGATTCGCGTTTGTTACGGTGTTGACCCCCGGGACCAGAGCTTTTGAATGTGCCCATTTCGCACTGGCGCATTAGGTCATCGTCTGTTAGGTCTAGGTAACCGCCGTAATTGGGGATGGCGGCAGCGGCGGTGGCGGTGGTGGTTGTGGAGCGAAGTGAGATTGGGTGAGTGTTGAGGTTGAGAAAGGAAGAGGATATTTTGGAGTGGAAAAATGGATAATGGTGATGATGATGATGAAGAAAGTGGTGGTGTGAATAGTTGTGAGATTTTGTTGTGAATTGTTTTGTGAGAGTGAGAATGAATGTTGGTTTGAAGAGGAGTTTGTGTGCCATTGAATTGTTTTGAGACACTGTTTCGTTCTAAATTCATTCACAGCCTCYTGTACCCCCGAC
TARGET=727,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04880 Ps_contig_mira-and-tgicl-ass_franssen_35011
SEQUENCE=TTAACTAAATAGAGAGAGAGAGAGAGAGAGNGAGAAACCTTGCTGCAATAGTCCCCACTCACTTTTGAATCTTCTTTCACTCTTCCTTTTTGATCTCAAATTATGCTCATTTTTCATCTTCAATTGCATACACTCACTCACCTTTTAACTCTTGTGGATATCAAGAACTTTAATATGTTGCTCTTGTTCATTCTTCTTCTTTGTTTGCGTGATTCCCATGGCAGTAGAGTTGATCATGCGCAAGGTCGCATTCAATTGGATAACCGTGTGGGTTCCATGTTCCCCCAAATCGCACCAGTTGGTGCTCCGCAAGGACTGCAACCCTTTCTTCCGCTTATTGCGCCTTCGCCGTTGACTCCATTCACCAATATTTCTATCCCAAAGTTATCAGGACTCTGCACTTTGAACTTCACCGCTGCCAGAAGTTTGCTAAGTGTGACGGCAATTGACTGCTGGGGATCTTTTGCGCCTGTTCTGGCTAACGTAATATGCTGTCCACAATTGGAAGCCACTCTCACTGTTCTCATAGGTCAATCCAGTAAATATACCAATGTACTTGCCTTAAACGGGACCGCTGCTAAACATTGCCTTTCGGACGTCGAGCAAATTTTAATGGGGCAGGGTGCAAATGGTGATCTGAAGCAAATATGTTCGGTTGATTCTCTTGAATCTGACGGAGGCATCTTGCCCGGTAAAACACGTGAATGATTTTTATGACATGGTGAATACTTCTAAGCTGCTTAAAGCGTGTGAGAACATTGATCCCGTGAAAGAATGCTGCTACCAATATTGTCACAGTGCTATATTAGAAGCAGCTACAAAACTTGCATCAAAGGGTTCTAATGCTTTGGACTCAGACGCATCACGTATTCTACCCGAACATTCAATTCGGGTCAATGATTGTAGAAATATAGTACTCCGGTGGATAGCTAGTAAGCTTGAGCCGTCTCACGGAAAGAAAGTTCTCAGAGGATTGTCTAATTGCAATATCAACAAAGTTTGTCCACTTGTTTTGCCTGACGCAAAGCATGTTGCCAAGGGTTGCGGCAACGGGATAAGTAACAAAAAAGCCTGCTGTGATACCATGGAAAGTTATGTGTCTCACTTGCAGAAGCAGAGTTTCATTACTAACTTGCAAGCTTTGGGATGTGCAGAAACTTTGGCAATGAAATTAAAAAAGTCGAAAATTGTCGATGATGTTTATAGTCTTTGTCACATAAGCCTTAAGGATTTTTCACTCCAAGTTGCAAATCAAGAATATGGCTGTCTATTACCTAGTTTGCCTTCAGACGCCGTGTTCGACCAAACTTCTGGCGTTAGCTTCCTTTGTGATCTAAACGACAATATCCCAGCTCCCTGGCCTTCTACATCTCAAGGATCTTCTTCATCGGCGTGCAATAAATCTATCATCAACATACCTGCCCTTCCTGCTGCCGCAGCATCTGGTCAAAGTTGTCTCTACAGTCACGACGTGCTGATATTCCTTCTTGTCAATTTATTCTTTCTCGTAATGGCACTCGTGTAACTTGCGAGAGCTTTCTTGTTAGTTAGACGCAGCCTTACTAGTCCTTCAACGATAAGGGCTTTCGGATGGATAGATGTTAATGTCAAAGGCTAATTGTCATTCAAATATAGCTGGTTTTATGACAGAGAGATCCGAGTTGGAGGTGAAGCCTGATGTGTTTCTGATCTTATTGATTAATGATTATTATGTGCTTGTGCAATAATTTTTTTGTATATATGAAATTTAGAAAGCATAGGTTATACAATAACCTACATGTCAGAGATTGAAATGTATACCTGAATTAATGTTGAAGGTGGTTTTGTAATTAGTAGTACTTAGTGTTGTTATGTAATGTTTAATGAATTTTCTTTAACCAGTAAACTATATATTACTTTAATTATTATNACT
TARGET=11,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04881 Ps_contig_mira-and-tgicl-ass_franssen_35018
SEQUENCE=ATTGTTTGCAAACCATCAGAATCCGCCATTGAATTCGCTCTCAAGATCGCCAACCTTAACCCTCAAAGAACCTTGTTCTTTGAGGACAGTGTCCGTAACATACAAGCTGGAAAACGTGTGGGGCTAGACACAGTGTTGGTTGGTACATCACAAAGAGTTAAAGGTGCAGATTATGCATTGGAAAGTATTCACAATCTTAGGGAAGCTGTGCCTGAATTATGGGAAAATGACATAAAATCAGAAGTTGCATACCCTGGAAAGTTAACATCAGTTACAGCTTGAGAGAAAGATTATGGAGATATTACTATTATTATTATTATTACTCTTTTAATATTGTTTATTTAATAAATTTAAATTCATGTCACAAAGAAAAAAGTGAAGAGCATGAGGATTCAACAATTTCAAATGTAGTTGATGTAGATGTCTTTTGTAAGGTTATAATAAAATTAGTATATGAAATAAATTCTCATTTTTGNC
TARGET=306,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9988
PRIMER_LEFT_PENALTY=0.315086
PRIMER_RIGHT_PENALTY=0.683672
PRIMER_LEFT_SEQUENCE=GCCTGAATTATGGGAAAATGAC
PRIMER_RIGHT_SEQUENCE=TTGTTGAATCCTCATGCTCTTC
PRIMER_LEFT=209,22
PRIMER_RIGHT=398,22
PRIMER_LEFT_TM=59.685
PRIMER_RIGHT_TM=59.316
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=1.0308
PRIMER_LEFT_1_PENALTY=0.347129
PRIMER_RIGHT_1_PENALTY=0.683672
PRIMER_LEFT_1_SEQUENCE=AGAAGTTGCATACCCTGGAAAG
PRIMER_RIGHT_1_SEQUENCE=TTGTTGAATCCTCATGCTCTTC
PRIMER_LEFT_1=239,22
PRIMER_RIGHT_1=398,22
PRIMER_LEFT_1_TM=59.653
PRIMER_RIGHT_1_TM=59.316
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=1.0562
PRIMER_LEFT_2_PENALTY=0.372557
PRIMER_RIGHT_2_PENALTY=0.683672
PRIMER_LEFT_2_SEQUENCE=AATCAGAAGTTGCATACCCTGG
PRIMER_RIGHT_2_SEQUENCE=TTGTTGAATCCTCATGCTCTTC
PRIMER_LEFT_2=235,22
PRIMER_RIGHT_2=398,22
PRIMER_LEFT_2_TM=60.373
PRIMER_RIGHT_2_TM=59.316
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=1.0699
PRIMER_LEFT_3_PENALTY=0.386267
PRIMER_RIGHT_3_PENALTY=0.683672
PRIMER_LEFT_3_SEQUENCE=GTGCAGATTATGCATTGGAAAG
PRIMER_RIGHT_3_SEQUENCE=TTGTTGAATCCTCATGCTCTTC
PRIMER_LEFT_3=163,22
PRIMER_RIGHT_3=398,22
PRIMER_LEFT_3_TM=59.614
PRIMER_RIGHT_3_TM=59.316
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=1.0908
PRIMER_LEFT_4_PENALTY=0.315086
PRIMER_RIGHT_4_PENALTY=0.775718
PRIMER_LEFT_4_SEQUENCE=GCCTGAATTATGGGAAAATGAC
PRIMER_RIGHT_4_SEQUENCE=TGTTGAATCCTCATGCTCTTCA
PRIMER_LEFT_4=209,22
PRIMER_RIGHT_4=397,22
PRIMER_LEFT_4_TM=59.685
PRIMER_RIGHT_4_TM=60.776
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_04882 Ps_contig_mira-and-tgicl-ass_franssen_35025
SEQUENCE=AACGCAGAGTAACGTCGGGGAAACACCTTCCATTTTCTATCCCTTTTCATGTGTCACATACATTATCAGAACTCTTTTGTAGGATCTTAAACAATGGAGAGTAGTACTACAAGCTCTGAACTCCCTCCTGGCTTTAGATTTCACCCAACTGATGAAGAATTGATTGTGTATTACCTTTGTAATCAAGCCACATCAAAGCCATGTCCTGCTTCTATCATACCAGAAGTTGATATCTATAAATTTGATCCATGGGAATTGCCAGATAAATCGGAGTTTGCAGAGAATGAATGGTATTTCTTTAGTCCAAGAGAAAGAAAGTACCCAAATGGGGTGAGGCCTAATAGAGCAACTTTATCTGGTTACTGGAAAGCTACTGGCACAGACAAACCAATCAACACTGGATCAAAGCAAATTGGAGTCAAAAAATCTTTAGTCTTTTACAAGGGTAGGCCACCAAAGGGAATCAAAACTAATTGGATCATGCACGAGTATCGATTAATCGGATCACAAAAACAAACTAGCAAACATATTGGATCCATGAGGCTAGATGACTGGGTTTTGTGCAGGATCTATAAGAAGAAACACATGCAAAAAACATCGCAGCAAAAAGAGGACTATTCAACTCTTCAGTTCAATGATTCTACGATAGCGAATAACAACGAACAAGAAAGGATGAACATGAACCTGAACCTTACAAGAAGTTGTTCACTTACTTATCTTTTAGATATGAATTACTTTACTCCAATCTTATCTGATGGTTCAACTTTTGATTTTCAAATCAATAATTCAAATATTGGGATAGACCCTTTTGTAAAACCTCAGCAAGTTGAAATGAGTAACCATTATGAAGCAGATTCGATCACAAATCAGTCATTCTTTGTGAAGCAAATGTATAATTCTTTAGGATAAGAATTTAGATTGGCTAGATATGTTTAAAGAACTATGTCATTTTTATTTATTTATAAGTAGAAATTGAACTATGTCAAATATATTTTATACCATAGAATAGAATGGATTTTCATTTTCAATCCTTTGTTGACACTGTTTATACAAATTGTAACATTTTTAATGGCTCTTAATAAAAATTCACATATGGAATCACCTTCCCTAAACCTTAGACATGTCC
TARGET=951,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0768
PRIMER_LEFT_PENALTY=0.797753
PRIMER_RIGHT_PENALTY=0.279044
PRIMER_LEFT_SEQUENCE=GCAGATTCGATCACAAATCAGT
PRIMER_RIGHT_SEQUENCE=AAGGTTTAGGGAAGGTGATTCC
PRIMER_LEFT=849,22
PRIMER_RIGHT=1115,22
PRIMER_LEFT_TM=59.202
PRIMER_RIGHT_TM=59.721
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=1.2136
PRIMER_LEFT_1_PENALTY=0.797753
PRIMER_RIGHT_1_PENALTY=0.415851
PRIMER_LEFT_1_SEQUENCE=GCAGATTCGATCACAAATCAGT
PRIMER_RIGHT_1_SEQUENCE=GGTTTAGGGAAGGTGATTCCAT
PRIMER_LEFT_1=849,22
PRIMER_RIGHT_1=1113,22
PRIMER_LEFT_1_TM=59.202
PRIMER_RIGHT_1_TM=60.416
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=1.8406
PRIMER_LEFT_2_PENALTY=0.797753
PRIMER_RIGHT_2_PENALTY=1.042869
PRIMER_LEFT_2_SEQUENCE=GCAGATTCGATCACAAATCAGT
PRIMER_RIGHT_2_SEQUENCE=AGGTTTAGGGAAGGTGATTCCA
PRIMER_LEFT_2=849,22
PRIMER_RIGHT_2=1114,22
PRIMER_LEFT_2_TM=59.202
PRIMER_RIGHT_2_TM=61.043
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=1.9045
PRIMER_LEFT_3_PENALTY=1.488657
PRIMER_RIGHT_3_PENALTY=0.415851
PRIMER_LEFT_3_SEQUENCE=AACCTCAGCAAGTTGAAATGAG
PRIMER_RIGHT_3_SEQUENCE=GGTTTAGGGAAGGTGATTCCAT
PRIMER_LEFT_3=814,22
PRIMER_RIGHT_3=1113,22
PRIMER_LEFT_3_TM=58.511
PRIMER_RIGHT_3_TM=60.416
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=1.9706
PRIMER_LEFT_4_PENALTY=0.797753
PRIMER_RIGHT_4_PENALTY=1.172839
PRIMER_LEFT_4_SEQUENCE=GCAGATTCGATCACAAATCAGT
PRIMER_RIGHT_4_SEQUENCE=GGTTTAGGGAAGGTGATTCCA
PRIMER_LEFT_4=849,22
PRIMER_RIGHT_4=1113,21
PRIMER_LEFT_4_TM=59.202
PRIMER_RIGHT_4_TM=60.173
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_04883 Ps_contig_mira-and-tgicl-ass_franssen_35028
SEQUENCE=TAAAAAGGGTAACTTACCATTTGATCATGAACCTGCAATCTTTTCAGAGTTTGTGGAGTTTGCAGTGGTAAACATTAATTATGAACACAATGGACCAGTAATTCCAGATGATAAGAATGCGTGCAACATAATATTATGCATGTTTTTGACTCCCTACCTATGATGTAAAAGTGTCTAACTACAAAAAAATCACAAATCTATTCCAAAATGCAGTCTGCATAGGTTCAATAGTCTCACCCAAACAAGTCATCATCATCATCATCATCCTCATCGGCATTGCTTGGATTACTGTCAGATTTTCCACTTAATGTATTCTCTTTTCCATTCTCCTTAGCTGCTTGAGCTTGTTCACCACTATTGATAGGTGGGTATGCCCATGCTGATCCAACTTTGACGGATAATGATTCTCCATAAAGGTCGTCATAAATCCCAGTTTTAACGGGGTTTTTGATCTTTTTAAGGACTTCTTGTAGTTTATCAGTCACACCTTTTTGGCATGAATCTGCCTCCTTAACAGAGGACTGCTCTTTCCCCTTTGGAATTACAGTTATCTGTACAAGAGATTCATATTTACCAACAAGACTTCCTTCTTTAACACCAACTGGACCAGGTTCTGTTTCTACATCTACACCATCTGAAATTCCAACAACCTGAACTAATTCCCCCCCAACTTGATCCCTGAAGGAAACTCTTGTCTTCTTAATTCTCTTAGCAGCTCTTTCTGATTGCCAATCCTTGTTTTGGCCACTTGCTGAACCACCAAGCTCATTGGATTTGGGCACTAGATCAGACTTGTTGATGCCATAACGATTCAGCAGCGTATTATAAGNAA
TARGET=247,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1115
PRIMER_LEFT_PENALTY=0.073989
PRIMER_RIGHT_PENALTY=0.037486
PRIMER_LEFT_SEQUENCE=GTGGAGTTTGCAGTGGTAAACA
PRIMER_RIGHT_SEQUENCE=TGAACAAGCTCAAGCAGCTAAG
PRIMER_LEFT=52,22
PRIMER_RIGHT=350,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.963
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=0.1331
PRIMER_LEFT_1_PENALTY=0.095629
PRIMER_RIGHT_1_PENALTY=0.037486
PRIMER_LEFT_1_SEQUENCE=TTCCAGATGATAAGAATGCGTG
PRIMER_RIGHT_1_SEQUENCE=TGAACAAGCTCAAGCAGCTAAG
PRIMER_LEFT_1=101,22
PRIMER_RIGHT_1=350,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.963
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.1404
PRIMER_LEFT_2_PENALTY=0.073989
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=GTTTGTGGAGTTTGCAGTGGTA
PRIMER_RIGHT_2_SEQUENCE=AAGCTCAAGCAGCTAAGGAGAA
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=345,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=0.1404
PRIMER_LEFT_3_PENALTY=0.073989
PRIMER_RIGHT_3_PENALTY=0.066394
PRIMER_LEFT_3_SEQUENCE=GTGGAGTTTGCAGTGGTAAACA
PRIMER_RIGHT_3_SEQUENCE=AAGCTCAAGCAGCTAAGGAGAA
PRIMER_LEFT_3=52,22
PRIMER_RIGHT_3=345,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=0.1620
PRIMER_LEFT_4_PENALTY=0.095629
PRIMER_RIGHT_4_PENALTY=0.066394
PRIMER_LEFT_4_SEQUENCE=TTCCAGATGATAAGAATGCGTG
PRIMER_RIGHT_4_SEQUENCE=AAGCTCAAGCAGCTAAGGAGAA
PRIMER_LEFT_4=101,22
PRIMER_RIGHT_4=345,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=59.934
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_04884 Ps_contig_mira-and-tgicl-ass_franssen_35035
SEQUENCE=GTCGGGGATTAACATTTTGATAAAATTATTACTACTTCAAATTTAACCAAATTTAAGTGATCTTTCTTCATTTCCATGCTCGCATGGCTTCTGTTGTACCCATTCTCCATAACCTGCCATCTCTCTCCCATTCTCAACCTCTTTTTTTAAGATCCACCAACCACAAATCAAAATTCCTCATCCTTTCAAGAAAACCTAATAACTCACCAATATTACTTCTCAAATGTTCTTCTTCTTCTTCCTCTTCTTCCATGCTAAACAAACTAGAAGAAGATGAAGAAGACATATTAGAAGACGTCGTCGGAGAACAAGAATTATCACCACCACAACCATCACCGCCGCTACCTCCGTTGAGCCAAGTATGGGAAGAAATCCAAGGTAAGAACAACTGGGAAGGCTTGTTAGATCCGATGGATCCCATTCTCCGGAAAGAAATCACTCGCTACGGCGAGTTTGCTCAAGCATGCTACGATTTCCTTCGTACTTTGATCCACACTACAAAATCTGTCGGAACCTGCAAGTATCA
TARGET=227,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0267
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.022341
PRIMER_LEFT_SEQUENCE=GCTTCTGTTGTACCCATTCTCC
PRIMER_RIGHT_SEQUENCE=TGATGGTTGTGGTGGTGATAAT
PRIMER_LEFT=86,22
PRIMER_RIGHT=334,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.978
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.0385
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=GCTTCTGTTGTACCCATTCTCC
PRIMER_RIGHT_1_SEQUENCE=ATTTCTTCCCATACTTGGCTCA
PRIMER_LEFT_1=86,22
PRIMER_RIGHT_1=372,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.0385
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=GGCTTCTGTTGTACCCATTCTC
PRIMER_RIGHT_2_SEQUENCE=ATTTCTTCCCATACTTGGCTCA
PRIMER_LEFT_2=85,22
PRIMER_RIGHT_2=372,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.0655
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.061184
PRIMER_LEFT_3_SEQUENCE=GCTTCTGTTGTACCCATTCTCC
PRIMER_RIGHT_3_SEQUENCE=TCTTACCTTGGATTTCTTCCCA
PRIMER_LEFT_3=86,22
PRIMER_RIGHT_3=383,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=59.939
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.0655
PRIMER_LEFT_4_PENALTY=0.004359
PRIMER_RIGHT_4_PENALTY=0.061184
PRIMER_LEFT_4_SEQUENCE=GGCTTCTGTTGTACCCATTCTC
PRIMER_RIGHT_4_SEQUENCE=TCTTACCTTGGATTTCTTCCCA
PRIMER_LEFT_4=85,22
PRIMER_RIGHT_4=383,22
PRIMER_LEFT_4_TM=60.004
PRIMER_RIGHT_4_TM=59.939
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_04885 Ps_contig_mira-and-tgicl-ass_franssen_35045
SEQUENCE=AGCTTGGAGAGACTGTTAGAAGTTTACCTCATTGTCATCACATGTTTCATCTACCTTGCATAGACATGTGGCTCTTGAGGCATGGTTCTTGCCCATTATGCAGAAGGGATCTGTAATTTTGTATATGAATATGAGAAGAGTATTTTTTTTGTTTTGTTTTGTTTATAAGGTAATTTAATTCTAGGTCTTACTTACAGTTGATTATAGCTACTGAGAGGTAATGCAGTTATGTGATTGTACTAAAATTAGTGTAAAGAAAATTTTGAGATGAACCATTTTTCAATTGTTGTATATGTACCATATCAGCATATGAGTAGATGACTATATCCTAAAAGG
TARGET=147,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.7672
PRIMER_LEFT_PENALTY=0.242742
PRIMER_RIGHT_PENALTY=3.524425
PRIMER_LEFT_SEQUENCE=CATAGACATGTGGCTCTTGAGG
PRIMER_RIGHT_SEQUENCE=TCACATAACTGCATTACCTCTCAG
PRIMER_LEFT=58,22
PRIMER_RIGHT=233,24
PRIMER_LEFT_TM=59.757
PRIMER_RIGHT_TM=58.476
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=3.8644
PRIMER_LEFT_1_PENALTY=0.340007
PRIMER_RIGHT_1_PENALTY=3.524425
PRIMER_LEFT_1_SEQUENCE=CCATTATGCAGAAGGGATCTGT
PRIMER_RIGHT_1_SEQUENCE=TCACATAACTGCATTACCTCTCAG
PRIMER_LEFT_1=92,22
PRIMER_RIGHT_1=233,24
PRIMER_LEFT_1_TM=60.340
PRIMER_RIGHT_1_TM=58.476
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=142
PRIMER_PAIR_PENALTY_2=3.8646
PRIMER_LEFT_2_PENALTY=0.340219
PRIMER_RIGHT_2_PENALTY=3.524425
PRIMER_LEFT_2_SEQUENCE=CTACCTTGCATAGACATGTGGC
PRIMER_RIGHT_2_SEQUENCE=TCACATAACTGCATTACCTCTCAG
PRIMER_LEFT_2=50,22
PRIMER_RIGHT_2=233,24
PRIMER_LEFT_2_TM=59.660
PRIMER_RIGHT_2_TM=58.476
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=3.9683
PRIMER_LEFT_3_PENALTY=0.242742
PRIMER_RIGHT_3_PENALTY=3.725572
PRIMER_LEFT_3_SEQUENCE=CATAGACATGTGGCTCTTGAGG
PRIMER_RIGHT_3_SEQUENCE=TCACATAACTGCATTACCTCTCAGT
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=233,25
PRIMER_LEFT_3_TM=59.757
PRIMER_RIGHT_3_TM=59.274
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=4.0656
PRIMER_LEFT_4_PENALTY=0.340007
PRIMER_RIGHT_4_PENALTY=3.725572
PRIMER_LEFT_4_SEQUENCE=CCATTATGCAGAAGGGATCTGT
PRIMER_RIGHT_4_SEQUENCE=TCACATAACTGCATTACCTCTCAGT
PRIMER_LEFT_4=92,22
PRIMER_RIGHT_4=233,25
PRIMER_LEFT_4_TM=60.340
PRIMER_RIGHT_4_TM=59.274
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=142
=
PRIMER_SEQUENCE_ID=SSR_04886 Ps_contig_mira-and-tgicl-ass_franssen_35052
SEQUENCE=GAATCCGACTTTTCCGCCGCGAAGATCGGTAATGTTGACTAGTCCCGAGGCTCCAGGTGCGGAGCCTGTGGCTTGGAACAATGTGGAAACAAGAGCGGTTCCGTTTGTGATTTGGTGAAGTTTTTTGGCGTCGAAGTAGTCGACGAGAACGTGGAGGGAGAGGATGTTTTTCACGGTGTAGATCGAGAGGTCTTTTGCGATGAGGTCGTCCATGGCGGCATTGTTGACGGCGAGGACGGTGATTGTGTTGTGACGGTCGATTTCGGAAGCGAGGCGCGTTAGGGTTAGATAGTGGTTGAAGGTGGAGAACTCAGGGTTTTGGGAGAGAATGTGTGAGATGTTGTGTGCGGTGGAGGTTGTGAGAACGGCGGAGAGGATTAAAAGCGCCGCCGAGAAGGCCGCCATTGTTGGTGGTCGGTGCATGTGGTAAGTAGAAGAAGAAGAATGAGATCTGTAAAGAGTGAAGTGAAGACAGTGCAGTGTGAGAATGAGAACTGAGAAGTNAAGAATTTGAGACT
TARGET=433,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6825
PRIMER_LEFT_PENALTY=0.348961
PRIMER_RIGHT_PENALTY=0.333520
PRIMER_LEFT_SEQUENCE=ACTCAGGGTTTTGGGAGAGAAT
PRIMER_RIGHT_SEQUENCE=TTCTCACACTGCACTGTCTTCA
PRIMER_LEFT=308,22
PRIMER_RIGHT=487,22
PRIMER_LEFT_TM=60.349
PRIMER_RIGHT_TM=59.666
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=180
PRIMER_PAIR_PENALTY_1=0.7520
PRIMER_LEFT_1_PENALTY=0.418525
PRIMER_RIGHT_1_PENALTY=0.333520
PRIMER_LEFT_1_SEQUENCE=CGCGTTAGGGTTAGATAGTGGT
PRIMER_RIGHT_1_SEQUENCE=TTCTCACACTGCACTGTCTTCA
PRIMER_LEFT_1=274,22
PRIMER_RIGHT_1=487,22
PRIMER_LEFT_1_TM=59.581
PRIMER_RIGHT_1_TM=59.666
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=0.8423
PRIMER_LEFT_2_PENALTY=0.508801
PRIMER_RIGHT_2_PENALTY=0.333520
PRIMER_LEFT_2_SEQUENCE=AGGCGCGTTAGGGTTAGATAGT
PRIMER_RIGHT_2_SEQUENCE=TTCTCACACTGCACTGTCTTCA
PRIMER_LEFT_2=271,22
PRIMER_RIGHT_2=487,22
PRIMER_LEFT_2_TM=60.509
PRIMER_RIGHT_2_TM=59.666
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=217
PRIMER_PAIR_PENALTY_3=1.2073
PRIMER_LEFT_3_PENALTY=0.873742
PRIMER_RIGHT_3_PENALTY=0.333520
PRIMER_LEFT_3_SEQUENCE=GAAGGTGGAGAACTCAGGGTTT
PRIMER_RIGHT_3_SEQUENCE=TTCTCACACTGCACTGTCTTCA
PRIMER_LEFT_3=297,22
PRIMER_RIGHT_3=487,22
PRIMER_LEFT_3_TM=60.874
PRIMER_RIGHT_3_TM=59.666
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=1.2478
PRIMER_LEFT_4_PENALTY=0.348961
PRIMER_RIGHT_4_PENALTY=0.898847
PRIMER_LEFT_4_SEQUENCE=ACTCAGGGTTTTGGGAGAGAAT
PRIMER_RIGHT_4_SEQUENCE=TCTCACACTGCACTGTCTTCAC
PRIMER_LEFT_4=308,22
PRIMER_RIGHT_4=486,22
PRIMER_LEFT_4_TM=60.349
PRIMER_RIGHT_4_TM=59.101
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_04887 Ps_contig_mira-and-tgicl-ass_franssen_35061
SEQUENCE=CAACGCAGAGTACGGCGGGATTGCTAACTAATAAATGCCACCCAACTAACTCCCTANNCNNNACCATACTCTCAAACACATACATACTTACATTTTCTCCCATTCTCATCTTCTTCAAATCTCAACTCACTCATCAACAACAACAACAAAAAAGCACCAACTTTTTTACTACTCATCTTCTTCCTCTGTTTTTTTTATTTAGCAAAAATGGACACAAGAATGCAAAACATAAACACACAGTTCCAAAACAGTACCGATATTGGATCATGTTTATCAAATCTCATCTTATCAACTACAACAAACACACTCGACTCTATTTTCTCTGATATTCCATCATCAAACACCACCAACACCACCACCAACAACAACAATTTCTTGGGTTGTTCAAATTTTGAACCTTTAGGTTCCTCGGTTTATCTTCGTCAAAGAGACATTCTTCAAAAATTCTACGAAGAAACTCGATTAACCCCCTCTTTCATTCCATCTTCATTTTCAAACACACCTTTATACACAACAACAACAAATTCAATTTCAAGTGCAAGTTCGAGTTTGTTCACTTCTTTGGTGAACAATCCTTTGAAGAAGAAGCTATACAGAGGAGTGAGACAGAGACACTGGGGAAAATGGGTTGCTGAGATTCGACTTCCTCAGAATAGAATGAGAGTTTGG
TARGET=135,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3035
PRIMER_LEFT_PENALTY=0.236697
PRIMER_RIGHT_PENALTY=0.066849
PRIMER_LEFT_SEQUENCE=TAAATGCCACCCAACTAACTCC
PRIMER_RIGHT_SEQUENCE=TCGGTACTGTTTTGGAACTGTG
PRIMER_LEFT=31,22
PRIMER_RIGHT=256,22
PRIMER_LEFT_TM=60.237
PRIMER_RIGHT_TM=60.067
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=226
PRIMER_PAIR_PENALTY_1=0.5453
PRIMER_LEFT_1_PENALTY=0.478439
PRIMER_RIGHT_1_PENALTY=0.066849
PRIMER_LEFT_1_SEQUENCE=GGCGGGATTGCTAACTAATAAA
PRIMER_RIGHT_1_SEQUENCE=TCGGTACTGTTTTGGAACTGTG
PRIMER_LEFT_1=13,22
PRIMER_RIGHT_1=256,22
PRIMER_LEFT_1_TM=59.522
PRIMER_RIGHT_1_TM=60.067
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=1.1631
PRIMER_LEFT_2_PENALTY=0.236697
PRIMER_RIGHT_2_PENALTY=0.926360
PRIMER_LEFT_2_SEQUENCE=TAAATGCCACCCAACTAACTCC
PRIMER_RIGHT_2_SEQUENCE=CGGTACTGTTTTGGAACTGTGT
PRIMER_LEFT_2=31,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=60.237
PRIMER_RIGHT_2_TM=59.074
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=1.1662
PRIMER_LEFT_3_PENALTY=1.099308
PRIMER_RIGHT_3_PENALTY=0.066849
PRIMER_LEFT_3_SEQUENCE=GCCACCCAACTAACTCCCTAN
PRIMER_RIGHT_3_SEQUENCE=TCGGTACTGTTTTGGAACTGTG
PRIMER_LEFT_3=36,21
PRIMER_RIGHT_3=256,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=60.067
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.50
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.1750
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=1.2056
PRIMER_LEFT_4_PENALTY=1.138774
PRIMER_RIGHT_4_PENALTY=0.066849
PRIMER_LEFT_4_SEQUENCE=TCTCCCATTCTCATCTTCTTCA
PRIMER_RIGHT_4_SEQUENCE=TCGGTACTGTTTTGGAACTGTG
PRIMER_LEFT_4=95,22
PRIMER_RIGHT_4=256,22
PRIMER_LEFT_4_TM=58.861
PRIMER_RIGHT_4_TM=60.067
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_04888 Ps_contig_mira-and-tgicl-ass_franssen_35061
SEQUENCE=CAACGCAGAGTACGGCGGGATTGCTAACTAATAAATGCCACCCAACTAACTCCCTANNCNNNACCATACTCTCAAACACATACATACTTACATTTTCTCCCATTCTCATCTTCTTCAAATCTCAACTCACTCATCAACAACAACAACAAAAAAGCACCAACTTTTTTACTACTCATCTTCTTCCTCTGTTTTTTTTATTTAGCAAAAATGGACACAAGAATGCAAAACATAAACACACAGTTCCAAAACAGTACCGATATTGGATCATGTTTATCAAATCTCATCTTATCAACTACAACAAACACACTCGACTCTATTTTCTCTGATATTCCATCATCAAACACCACCAACACCACCACCAACAACAACAATTTCTTGGGTTGTTCAAATTTTGAACCTTTAGGTTCCTCGGTTTATCTTCGTCAAAGAGACATTCTTCAAAAATTCTACGAAGAAACTCGATTAACCCCCTCTTTCATTCCATCTTCATTTTCAAACACACCTTTATACACAACAACAACAAATTCAATTTCAAGTGCAAGTTCGAGTTTGTTCACTTCTTTGGTGAACAATCCTTTGAAGAAGAAGCTATACAGAGGAGTGAGACAGAGACACTGGGGAAAATGGGTTGCTGAGATTCGACTTCCTCAGAATAGAATGAGAGTTTGG
TARGET=360,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1891
PRIMER_LEFT_PENALTY=0.066849
PRIMER_RIGHT_PENALTY=0.122257
PRIMER_LEFT_SEQUENCE=CACAGTTCCAAAACAGTACCGA
PRIMER_RIGHT_SEQUENCE=CTTTGACGAAGATAAACCGAGG
PRIMER_LEFT=235,22
PRIMER_RIGHT=427,22
PRIMER_LEFT_TM=60.067
PRIMER_RIGHT_TM=60.122
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=193
PRIMER_PAIR_PENALTY_1=0.2202
PRIMER_LEFT_1_PENALTY=0.066849
PRIMER_RIGHT_1_PENALTY=0.153359
PRIMER_LEFT_1_SEQUENCE=CACAGTTCCAAAACAGTACCGA
PRIMER_RIGHT_1_SEQUENCE=AGGGGGTTAATCGAGTTTCTTC
PRIMER_LEFT_1=235,22
PRIMER_RIGHT_1=471,22
PRIMER_LEFT_1_TM=60.067
PRIMER_RIGHT_1_TM=59.847
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=0.2202
PRIMER_LEFT_2_PENALTY=0.066849
PRIMER_RIGHT_2_PENALTY=0.153359
PRIMER_LEFT_2_SEQUENCE=CACAGTTCCAAAACAGTACCGA
PRIMER_RIGHT_2_SEQUENCE=GAGGGGGTTAATCGAGTTTCTT
PRIMER_LEFT_2=235,22
PRIMER_RIGHT_2=472,22
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=59.847
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.2202
PRIMER_LEFT_3_PENALTY=0.066849
PRIMER_RIGHT_3_PENALTY=0.153359
PRIMER_LEFT_3_SEQUENCE=CACAGTTCCAAAACAGTACCGA
PRIMER_RIGHT_3_SEQUENCE=AAGAGGGGGTTAATCGAGTTTC
PRIMER_LEFT_3=235,22
PRIMER_RIGHT_3=474,22
PRIMER_LEFT_3_TM=60.067
PRIMER_RIGHT_3_TM=59.847
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=0.4417
PRIMER_LEFT_4_PENALTY=0.066849
PRIMER_RIGHT_4_PENALTY=0.374887
PRIMER_LEFT_4_SEQUENCE=CACAGTTCCAAAACAGTACCGA
PRIMER_RIGHT_4_SEQUENCE=CGAAGATAAACCGAGGAACCTA
PRIMER_LEFT_4=235,22
PRIMER_RIGHT_4=421,22
PRIMER_LEFT_4_TM=60.067
PRIMER_RIGHT_4_TM=59.625
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=187
=
PRIMER_SEQUENCE_ID=SSR_04889 Ps_contig_mira-and-tgicl-ass_franssen_35061
SEQUENCE=CAACGCAGAGTACGGCGGGATTGCTAACTAATAAATGCCACCCAACTAACTCCCTANNCNNNACCATACTCTCAAACACATACATACTTACATTTTCTCCCATTCTCATCTTCTTCAAATCTCAACTCACTCATCAACAACAACAACAAAAAAGCACCAACTTTTTTACTACTCATCTTCTTCCTCTGTTTTTTTTATTTAGCAAAAATGGACACAAGAATGCAAAACATAAACACACAGTTCCAAAACAGTACCGATATTGGATCATGTTTATCAAATCTCATCTTATCAACTACAACAAACACACTCGACTCTATTTTCTCTGATATTCCATCATCAAACACCACCAACACCACCACCAACAACAACAATTTCTTGGGTTGTTCAAATTTTGAACCTTTAGGTTCCTCGGTTTATCTTCGTCAAAGAGACATTCTTCAAAAATTCTACGAAGAAACTCGATTAACCCCCTCTTTCATTCCATCTTCATTTTCAAACACACCTTTATACACAACAACAACAAATTCAATTTCAAGTGCAAGTTCGAGTTTGTTCACTTCTTTGGTGAACAATCCTTTGAAGAAGAAGCTATACAGAGGAGTGAGACAGAGACACTGGGGAAAATGGGTTGCTGAGATTCGACTTCCTCAGAATAGAATGAGAGTTTGG
TARGET=511,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1953
PRIMER_LEFT_PENALTY=0.153359
PRIMER_RIGHT_PENALTY=0.041981
PRIMER_LEFT_SEQUENCE=AACTCGATTAACCCCCTCTTTC
PRIMER_RIGHT_SEQUENCE=TGAACAAACTCGAACTTGCACT
PRIMER_LEFT=455,22
PRIMER_RIGHT=555,22
PRIMER_LEFT_TM=59.847
PRIMER_RIGHT_TM=59.958
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=0.1953
PRIMER_LEFT_1_PENALTY=0.153359
PRIMER_RIGHT_1_PENALTY=0.041981
PRIMER_LEFT_1_SEQUENCE=GAAACTCGATTAACCCCCTCTT
PRIMER_RIGHT_1_SEQUENCE=TGAACAAACTCGAACTTGCACT
PRIMER_LEFT_1=453,22
PRIMER_RIGHT_1=555,22
PRIMER_LEFT_1_TM=59.847
PRIMER_RIGHT_1_TM=59.958
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=103
PRIMER_PAIR_PENALTY_2=0.1953
PRIMER_LEFT_2_PENALTY=0.153359
PRIMER_RIGHT_2_PENALTY=0.041981
PRIMER_LEFT_2_SEQUENCE=AAGAAACTCGATTAACCCCCTC
PRIMER_RIGHT_2_SEQUENCE=TGAACAAACTCGAACTTGCACT
PRIMER_LEFT_2=451,22
PRIMER_RIGHT_2=555,22
PRIMER_LEFT_2_TM=59.847
PRIMER_RIGHT_2_TM=59.958
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=0.1953
PRIMER_LEFT_3_PENALTY=0.153359
PRIMER_RIGHT_3_PENALTY=0.041981
PRIMER_LEFT_3_SEQUENCE=GAAGAAACTCGATTAACCCCCT
PRIMER_RIGHT_3_SEQUENCE=TGAACAAACTCGAACTTGCACT
PRIMER_LEFT_3=450,22
PRIMER_RIGHT_3=555,22
PRIMER_LEFT_3_TM=59.847
PRIMER_RIGHT_3_TM=59.958
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.1953
PRIMER_LEFT_4_PENALTY=0.153359
PRIMER_RIGHT_4_PENALTY=0.041981
PRIMER_LEFT_4_SEQUENCE=GAAACTCGATTAACCCCCTCTT
PRIMER_RIGHT_4_SEQUENCE=ACAAACTCGAACTTGCACTTGA
PRIMER_LEFT_4=453,22
PRIMER_RIGHT_4=552,22
PRIMER_LEFT_4_TM=59.847
PRIMER_RIGHT_4_TM=59.958
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=100
=
PRIMER_SEQUENCE_ID=SSR_04890 Ps_contig_mira-and-tgicl-ass_franssen_35070
SEQUENCE=GGAGTCATATTAATTTTCTTCTTCTTATTATATATTCCCTNGAAATCAGCAGGTAGRAGAACGAATTATTAGCTATTTTAAAAGGTAATTAAAGAGAGAAATGGCAGAGAGAAACACAAGTAGCAGCAGAAGCAAGAAATGGTCTTTGAAGGGGATGACAGCTCTCGTCACCGGTGGATCCAAAGGAATCGGATATGATATCGTGGAGCAGTTAGCAGAGCTAGGAGCAACGGTGCACACTTGTGCGAGAAACGAAGCTGAACTCAGTGAATGCTTGCATCAATGGGCCACAAAAGGATACAAAGTTACTGGTTCGGTCTGCGATGTGTCATCTCGTCCACAGAGAGAGGAGCTTATAACTAGAGTCTCTTCTGAGTTTAATGGCAAACTCAGTATTCTTGTGAACAACGTGGGAACAAACATACACAAACAAACACTGGATATTACAGAAGAAGATTTTTCACTTCTGGTAAATATCAATCTTGAATCTGCTTTTCAGATAAGCCAGCTTTCACATCCACTCCTTAAAGCTTCAGAGTCTGCAAGTATTGTTTTCATATCTTCCGTTGGTGGTGTAGCATCAATAAATATAGGAACCATCTATAGTGCAACAAAAGCTGCAATAATCCAACTCACAAAAAATTTGGCATGCGAATGGGCTAAAGACAACATAAGGACTAATTGTGTTGCACCGGGACCAATCAGAACCCCTCTTGGTGAAGAACACTTAAAAGATGAAAAAGTTCTTAATGGTTTCACTTCAAGAACTCCTCTTGGACGAATAGGAGAACCAGACGAGGTGTCTTCTTTGGTGGCTTTCCTATGCTTACCTGCAGCCTCTTTCATAACCGGGCAGACCATTTGTATTGATGGTGGATTAACTGTCAATTGTCTCCTTATATAATAAGTTTGGCTTACACAAACAAGACTTTGAATGGAAAAGCTTATAATTTGAAGCTAGTAAAATAAACTTTTGTGCTGGCGTTTATTATTAAACTTGCACACAAGGCATGTTTTTATTTTATTTCAATCAGTTTTCAAGAAAAGATTATAAACATGTGTTCAATTGATTTAATCCGTCAAACTCATGTTTGTAGTAGTAGTATTTATGTTAATGTTAAACAATAAACAAATATTATCAANTTTTGTATTATCATTTAGATGAGTGAAAATTTCAAACAAAGTTGAATTGTTTTTGTGAGATATTGGATCGAACTCTAGTATGATTGAAAGGTAGCTTTATTGGTTCGACAATTCGACAGGATCTTAGCATGCTTAGTTAARGTCGGCTCACA
TARGET=1094,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04891 Ps_contig_mira-and-tgicl-ass_franssen_35071
SEQUENCE=TGATGATGATGATTGGTGGANAGGGGATGATAGCAATGAGAGGAAGAAGGTGGTTATGACGAGGAGGAGTATGAAAAGAAGAAGGACTNTTGGGGGTGGTGATGGTGATGATGCTGGAAAGGGGCCTCTTGATACTGGGAGGCCCAAGAGGGGGGTTCAGAATGGTAATGCTGGCGGCGGCGGTGGTGGTGACAATGTGCATGATGGTGAGGCTGTTGATCCTGCCTCTGCACCCGCGAAGCTGTCCAATGTTGAAGCTAGTAAGAAAGCTGTGTTGGGTGGATCGAGGAGGAGGGGTGCGGCCAATTTAGGTAAATTGGATCTGAATGTAGAGTTTAGCAATGAGGTGGAAGAAGCTGCTGCTGGCGGAGCGAAAGAACGGAATGTGAATGGGACTGGAAATCCTGAGGATAATATTGAAGGAATTGGGTTTTTTGAAGGGCTCGATGAGTTCCTTAGTAGCTTACCAATTCTAAA
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04892 Ps_contig_mira-and-tgicl-ass_franssen_35095
SEQUENCE=TATGATCTATTTCTAACTAACTAATGCATTTCCTATTTCATAGAAGCTAGTGGCAATCTGGATTTGGTATTTTTGGGAGGGGATTGAATGGTGGAGCCTTGTTACTTCATCCCGTCTTCGCTCCGATCTCAAATTCGCCGCGAAAAGCGAAACACGAAGGACCTTTCAAAATTGGCGCAACATCGTCTTGATGGTGGATATGGAACTCAGCAGGTTTGCTTTATCTGGATGAGGATATTTGGTCTTTGCATTGGACCAAGACTGGCTTCAAGNCCAATTAGCTTGCCAGGTGGTTCTTTCCGTTTCTCACCAAATTTGCAGCATACACCCTTAGAGGACTTGGGCCTTGT
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04893 Ps_contig_mira-and-tgicl-ass_franssen_35113
SEQUENCE=ATCAACGCAGATACGGGGNGGTCTTTCATTTCCCACCTAATCCTCTTCTTCACTCTCTTGAACATAAAATAAAATCCCTAGAAAAATGGGTTTTAATACATCACCTATTGCAGTGATCTTGATCGTCGTCACGATCTTCACTGTAACACTTGCCTCCGACATGTCTATCATTTCCTACGGCCAAACCCACACAGACAAGTCTTCCTGGAGAACCGAAGAGGAGGTGAAGTCTATCTACGAGGAGTGGCGCGTGAGGCATGAAAAGCTCAACAACAACAACAACAACCTGGGAGAAAACGAAAAGAGATTCGAAATCTTCAAAGACAACCTTAAGTTCATCGACGAACACAATGCCGAGAACCGGACATATAAGGTTGGGCTGAACCGGTTCGCTGATCTTAGCAACGAGGAGTACCGGTCCATGTATCTTGGATTCAGGGCTGACTCAAATCGGTTGGGAAAGGCTGTGACGAAGAGCAACCGTTATGCTGCAAGTGTTGGTGATAACTTGCCTGAATCTGTTGATTGGAGGAACAAAGGTGCGGTGGCCCAAGTCAAAAACCAAGGAGGTTGTGGTAGTTGCTGGGCTTTTTCAGCAATAGCTGCAGTGGAAGGGATTAACAAGATAGTCACAGACGAGCTTGTTTCACTATCAGAACAAGAACTGATTGATTGTGACAGAACCAATAATGCAGGTTGCGATGGCGGGCTCATGGAATATGCCTTTGAGTTCATCATAAACAATGGTGGCATTGACACTGATAAGGATTATCCCTATCGAGCTGTTGATGGTAAATGTGATCAAAACAAAGAAAAATGCGAGAGTTGTTACAATTGATGACTACGAACAGGTTCCTGCTAATGACGAGTTAGCCTTAAAAAAAGCTGTTGCAAATCAACCAATCAGCGTTGCCATTGAAGCAGCTGGTAGGGAATTCCAGTTATATGTATCAGGTATATTTACCGGAAAATGTGGGACATCATTAGACCATGGTGTTGCTGCCGTAGGGTATGGAACAGAAAACGGAGTTGATTATTGGATAGTGAGGAATTCATGGGGTAAGAGCTGGGGAGAAGCAGGATATGTAAAAATGGAGCGCAATATAGCAACGAGCGTGTCAGGGAAATGTGGAATTGCATTGCAGTCTTCCTATCCTATAAAGAAAGGCCAAAATCCTCCGAATCCGGGACCATCTCCTCCTTCACCTGTGAACCCGCCATCTGTTTGTAGTAGATCCTACAGTTGTGCTTCGGGCACTACTTGCTGTTGTACATTTGGATTTGGAAAACTATGCTTCTCATGGGGATGTTGTCCTCTTGAAGCTGCAGTTTGTTGTAAAGATCGATCAAGTTGTTGTCCTCATGACTATCCCGTTTGCAACACTCGCCAGGGGACCTGTCTTCGGAGTAAGGATAATCCATTCGGAGTGAAGGCAATGAAGAGAACTCTTGCTAAACCGCATTGGATGTTCGAAGGTCAGAATATGATAGATGTTGCTTAAGTAGATTATGACCAACTTGGTACTCATTTCCTTTCATTACCTTACGGTAAATCATATATGTAGAACTAAA
TARGET=268,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1035
PRIMER_LEFT_PENALTY=0.071684
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=AGTCTATCTACGAGGAGTGGCG
PRIMER_RIGHT_SEQUENCE=GTCAGCCCTGAATCCAAGATAC
PRIMER_LEFT=227,22
PRIMER_RIGHT=444,22
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=11.7000
PRIMER_RIGHT_END_STABILITY=5.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=0.1054
PRIMER_LEFT_1_PENALTY=0.073614
PRIMER_RIGHT_1_PENALTY=0.031769
PRIMER_LEFT_1_SEQUENCE=ACCCACACAGACAAGTCTTCCT
PRIMER_RIGHT_1_SEQUENCE=GTCAGCCCTGAATCCAAGATAC
PRIMER_LEFT_1=184,22
PRIMER_RIGHT_1=444,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.1090
PRIMER_LEFT_2_PENALTY=0.071684
PRIMER_RIGHT_2_PENALTY=0.037339
PRIMER_LEFT_2_SEQUENCE=AGTCTATCTACGAGGAGTGGCG
PRIMER_RIGHT_2_SEQUENCE=TCAGGCAAGTTATCACCAACAC
PRIMER_LEFT_2=227,22
PRIMER_RIGHT_2=515,22
PRIMER_LEFT_2_TM=59.928
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.1673
PRIMER_LEFT_3_PENALTY=0.071684
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=AGTCTATCTACGAGGAGTGGCG
PRIMER_RIGHT_3_SEQUENCE=AGTCAGCCCTGAATCCAAGATA
PRIMER_LEFT_3=227,22
PRIMER_RIGHT_3=445,22
PRIMER_LEFT_3_TM=59.928
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=219
PRIMER_PAIR_PENALTY_4=0.1679
PRIMER_LEFT_4_PENALTY=0.136083
PRIMER_RIGHT_4_PENALTY=0.031769
PRIMER_LEFT_4_SEQUENCE=CTATCATTTCCTACGGCCAAAC
PRIMER_RIGHT_4_SEQUENCE=GTCAGCCCTGAATCCAAGATAC
PRIMER_LEFT_4=164,22
PRIMER_RIGHT_4=444,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=59.968
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_04894 Ps_contig_mira-and-tgicl-ass_franssen_35116
SEQUENCE=TTGTTTTGTTTGATATTTGAAAAGACATAACAAAAACAAAACAAAGCAACCAAAATATAAGGTTTAATTTCGGCCAAAGCAGCGTTCACCGGGTCCACCGTAGCTGAGCTGAGCCGAGCCACTTCTCCGACGAACGAACTCCTTCTCTCTTCTTCTTCTCACACCAGATCAGCTGTTGTAGAGGGAAGAAGAGAGTGTTGCTGAGTGTAATCAAGAAGCTCAAGTTTGACACTTATTTTGTCGAAGTGGTTGTCCCTATCACCTGAAGAAGATCTTTAAGAAAGACGATTCTTTGGGTAAATTGTCTGTTATTAGGTTTTGTTGAGTGATAACAAAACCATTATGGCTGTTGCCGGGAGCGGCTGCAGCAGCAGCGAAGGCTCAACTTCATTCAATTGTTACAAAGTAGCAAACTTGACCGAAACCATATTGAACGCAACGCAAACTTCTAATTTGAAGGACCGATACATTCTTGGGGAGCAGTTGGGCTGGGGACAATTTGGTGTCATTAGAACATGCTCCGACAAAACGACTGGAGAGGTTTTGGCATGCAAATCAATTGCTAAAGATAGGTTGGTGACTTCGGATGATATGCAAAGTGTTAAACTTGAGATCGAGATCATGGCAAAGTTGTCTGGGCACCCGAATGTTGTAGATCTTAAGGCGGTTTACGAAGAGGAAGATTTCGTTCATTTGGTGATGGAGCTTTGCGCTGGAGGCGAGCTGTTTCACCTGTTAGAGAAGCACGGGAGGTTTTCTGAATCTGAAGGCATGGTTCTCTTTAGACATCTCATGCAGATGGTTTTGTACTGTCACGAAAACGGCGTCGTTCACCGAGATTTGAAGCCCGAAAACATTCTCTTAGCAACTAAATCCTTCTCATCTCCAATTAAATTGGCCGACTTTGGCCTTGCGACCTACATTAAGCCTGGACAGAGTTTGCATGGTTTAGTCGGGAGTCCATTTTATATAGCTCCAGAAGTATTGGCAGGTGCATATAACCAGGCTGCGGATATTTGGAGTGCTGGGGTTATTCTTTACATCCTGTTGAGTGGGATGCCGCCGTTTTGGGGGAAGACAAAGTCGCGAATATTCGAAGCTGTCAAGGCTGCTGATTTGAGGTTCCCATCTGAACCTTGGAATCGGATCTCGGTATCTGCTAAGGATTTAATAAGGCGAATG
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9870
PRIMER_LEFT_PENALTY=1.934226
PRIMER_RIGHT_PENALTY=0.052729
PRIMER_LEFT_SEQUENCE=GTTTAATTTCGGCCAAAGCAG
PRIMER_RIGHT_SEQUENCE=CAGCAACACTCTCTTCTTCCCT
PRIMER_LEFT=61,21
PRIMER_RIGHT=202,22
PRIMER_LEFT_TM=60.934
PRIMER_RIGHT_TM=60.053
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=142
PRIMER_PAIR_PENALTY_1=2.0927
PRIMER_LEFT_1_PENALTY=1.811673
PRIMER_RIGHT_1_PENALTY=0.281044
PRIMER_LEFT_1_SEQUENCE=ACTTCTCCGACGAACGAACTC
PRIMER_RIGHT_1_SEQUENCE=CAGGTGATAGGGACAACCACTT
PRIMER_LEFT_1=120,21
PRIMER_RIGHT_1=264,22
PRIMER_LEFT_1_TM=60.812
PRIMER_RIGHT_1_TM=60.281
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=2.1731
PRIMER_LEFT_2_PENALTY=1.811673
PRIMER_RIGHT_2_PENALTY=0.361394
PRIMER_LEFT_2_SEQUENCE=ACTTCTCCGACGAACGAACTC
PRIMER_RIGHT_2_SEQUENCE=TCTTCAGGTGATAGGGACAACC
PRIMER_LEFT_2=120,21
PRIMER_RIGHT_2=268,22
PRIMER_LEFT_2_TM=60.812
PRIMER_RIGHT_2_TM=60.361
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=2.2153
PRIMER_LEFT_3_PENALTY=1.934226
PRIMER_RIGHT_3_PENALTY=0.281044
PRIMER_LEFT_3_SEQUENCE=GTTTAATTTCGGCCAAAGCAG
PRIMER_RIGHT_3_SEQUENCE=CAGGTGATAGGGACAACCACTT
PRIMER_LEFT_3=61,21
PRIMER_RIGHT_3=264,22
PRIMER_LEFT_3_TM=60.934
PRIMER_RIGHT_3_TM=60.281
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=2.2509
PRIMER_LEFT_4_PENALTY=2.198196
PRIMER_RIGHT_4_PENALTY=0.052729
PRIMER_LEFT_4_SEQUENCE=TTTAATTTCGGCCAAAGCAG
PRIMER_RIGHT_4_SEQUENCE=CAGCAACACTCTCTTCTTCCCT
PRIMER_LEFT_4=62,20
PRIMER_RIGHT_4=202,22
PRIMER_LEFT_4_TM=60.198
PRIMER_RIGHT_4_TM=60.053
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_04895 Ps_contig_mira-and-tgicl-ass_franssen_35124
SEQUENCE=GATTCATATTATAAAAGAAAAGATCACAACTTTGCGAGAGAGAGAGAGAGAAAGCCTACGTGRRYTYTNCTTCAATTTTAGGACAAACCACTTCGTGAATCTCGATATATATAAAGTGTACCTCTCCGCCACCCCAATTTCAACTTCTAACTACATTTCAATTTCATTACACTCTGACTTTCTTTTTCTTTCTCTTTCGATCTTACTTCAACTAGGGTTTATTCTTACCGGAAAACGATCCATCCAAGTCAGCCCTAGCAGTGGTTCTCTATCTCTACCGAGCTGCCTTGCCAACCTCCATTAATGGGCTGCTTCAACTCTAAGCCGACAACACAGTTTCCTGTGAAGGAGGATCCTGTCATTCTTGCATCACAGACTGCTTTCACTGTCAGCGAGGTCGAAGCATTATTCGAGCTTTTTAAGAGCATCAGCAGTTCTGTTGTTGATGATGGACTAATAAGCAAGGAAGAATTTCAATTGGCAATCTTCAAGAATAAGAAAAAGGAAAATATCTTTGCAAATCGGATCTTTGATCTTTTCGATGTCAAGAAGAAGGGAGTTATTGATTTTGATGACTTCGTTAGATCTCTCAACGTCTTCCACCCAAATGCAGCAATAGAAGACAAGATAGAATTCTCATTTAAGCTCTATGATTTGCACAATACTGGTTTTATAGAGCGTCAAGAGGTCAAGCAAATGTTGATCGCACTTCTTTTCGAATCTGAAATGAAGTTGGCTGATGACGTGATAGAAACAATTCTCGACAAGACTTTCATGGACGCCGACCTGAACCAAGATGGTAAAATAGACATGGTCGAGTGGCAAAAATTTGTTTCTGAAAATCCATCACTGCTCAAAATCATGACCCTTCCCTACCTAAGGGACATAACAACTTCTTTTCCAAGTTTTGTATTTAATTCTAACGTGGATGAAATTGTTGCTTGAGAGCATATAAATATAGGTTTTGCAATTATTGAGAGAACACACAACATTAGGTTCTCAATTATTTAGAGAATTGAATGTGTTTGAATAGATGACATGCTTATTATAGTTAGCCTTTCTCATTGAGTTATTGTAATTTGTAAATCACATTCTCATTGAAATTTGAGTCAGGTTTTCCTTGTAACTTAATTTATTTATCAATACATATTCGAAACRTAAAATTTAAGATACT
TARGET=36,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04896 Ps_contig_mira-and-tgicl-ass_franssen_35126
SEQUENCE=GTCGGGGACCGATAAACCACTCACCACAACCATTTCTCTTCCCTAATCAAACCACTCTCACGCTCCATCTTCAACCAACACCATGATGTGGGAATGGGAACAAGACGAACACCACCACCACCGCCACAACAACGCCTCTCCAAAACCCGATCAAATCCCTGACCCTAATTCCTATCTCAATTTCGATTTCTTCTCGGCTCTCTCCAAACCCAAGGATTATTACGAGATATTGGAAGTCGATTACGATGCTGATGAGGATGCTATTCGCTCTAATTACATGCGTCTTGCTCTTAAATGGCACCCTGATAAACAGAAAGACCAGAATTCTGCTACGTCAATGTTTCAGCATATAAACGAGGCATATCAGGGATTATCTTAACCGTTACAAGGGTCTTATATTGACTTGCAATGGCCTCGGCGTAAAACAATCACTATGGTAAAGCAAACCAGAATCAAGTACATCCATCCAGTGCCTCTAGGACAATTTTTTGAAGTACCTGGCGCTGCATGCCATGCTCAGTAATGAAAACTCATGCTTATGATGTCCTGGAGGGATAGGTTCATGCTTTATGTATAAAAGAAACATAAACTAAATGCGATTTTCTACTTGTAAACGCCAAATGTTTTCTCATTCACTTTGCTTACTTTCAAATGCATAGATATATTTCTCACTTTTCTCTTTATGTGCTGATGAAAATTTTGTTCACTATCCTTATGTTGCATGAAATANTAAGATTT
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4136
PRIMER_LEFT_PENALTY=0.344527
PRIMER_RIGHT_PENALTY=0.069038
PRIMER_LEFT_SEQUENCE=CCACAACCATTTCTCTTCCCTA
PRIMER_RIGHT_SEQUENCE=CGTAATAATCCTTGGGTTTGGA
PRIMER_LEFT=23,22
PRIMER_RIGHT=223,22
PRIMER_LEFT_TM=60.345
PRIMER_RIGHT_TM=60.069
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=201
PRIMER_PAIR_PENALTY_1=0.4136
PRIMER_LEFT_1_PENALTY=0.344527
PRIMER_RIGHT_1_PENALTY=0.069038
PRIMER_LEFT_1_SEQUENCE=CCACAACCATTTCTCTTCCCTA
PRIMER_RIGHT_1_SEQUENCE=TCGTAATAATCCTTGGGTTTGG
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=224,22
PRIMER_LEFT_1_TM=60.345
PRIMER_RIGHT_1_TM=60.069
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.4396
PRIMER_LEFT_2_PENALTY=0.242742
PRIMER_RIGHT_2_PENALTY=0.196811
PRIMER_LEFT_2_SEQUENCE=GATAAACCACTCACCACAACCA
PRIMER_RIGHT_2_SEQUENCE=CCGAGAAGAAATCGAAATTGAG
PRIMER_LEFT_2=10,22
PRIMER_RIGHT_2=196,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=60.197
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=0.5413
PRIMER_LEFT_3_PENALTY=0.344527
PRIMER_RIGHT_3_PENALTY=0.196811
PRIMER_LEFT_3_SEQUENCE=CCACAACCATTTCTCTTCCCTA
PRIMER_RIGHT_3_SEQUENCE=CCGAGAAGAAATCGAAATTGAG
PRIMER_LEFT_3=23,22
PRIMER_RIGHT_3=196,22
PRIMER_LEFT_3_TM=60.345
PRIMER_RIGHT_3_TM=60.197
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.6174
PRIMER_LEFT_4_PENALTY=0.548390
PRIMER_RIGHT_4_PENALTY=0.069038
PRIMER_LEFT_4_SEQUENCE=AAACCACTCACCACAACCATTT
PRIMER_RIGHT_4_SEQUENCE=CGTAATAATCCTTGGGTTTGGA
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=223,22
PRIMER_LEFT_4_TM=60.548
PRIMER_RIGHT_4_TM=60.069
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_04897 Ps_contig_mira-and-tgicl-ass_franssen_35128
SEQUENCE=AAGAGTGGTATCAACGCAGAGTCAGTTAGAGGGTGTGATGTTATGGTTGTTAGAACGGTTTGTTACAGCAGGTTGAAGAAGAAGATATTTAGGTTAGAGGAGTTTAGAGAAGGTTGAGAGTATGAAGGTTGATGAAGGGTTAGAATGGAAGGTGATGGAGGATGAAGTTGTGAATTGTTAAATGATAGTGAAGATGATGGAGAATCGAATTGGAGCTTGGTTCAGGGTGAAGAAGAGATGGAAGAAAACATGAGAGTGAAGGTGGCGAGAAGTGAAGGAGAAGGAAGTGGAAAAAAGGGCCTGTTGAAGAAGATAGGGATCTCTTGGATGGTTATCCTTTGATTCCATTATCGTGATGCCCTCTTCGAAGCTACAATGGAGGTGAATTTCCTCGTACACTGTCTTCATTACCAGGATTTGATTAATGAAGTTGCCCCACAGTGATTCTATGTAGTGCGGTGTTACGGCGAACTGTTGCACAATTAGAGTCAGTCGCTTCGAGCAACATTGTATGGTCGACTCAGTAAAGGTCACATTGGAATAAGATTGGAGCTAGGATACAACGCATCAGTTTTAGATTGAAACGCAATGCGGTTCGAGATGCTTGGATTACTGGTTTGAATATGTGGGCCACAGTTGGTGAAGTGTGTCCTTTGGTCTTGGCCATTTATAGGTGTGGGTTTGGTTGGCTTTAGTTAGTTAGAGTTGTTTTCTAATTTAAACAAAATGAACATTAACAGTGGAAGGAAAATTCAAGGGACTGCAAATGCAATTACACTATTGTTGTGCTTATTAATGGAAATTCAGTGGGTTAGCTTGGAATGTATTAATTGTTATGTTACTTTGAAATGATGTATGGTTGTAATTGAATTGAAATGGAGAACGAAATGCATGTGAGAATTGATCCATGTAATGTGAAAACATATATACTCTTAGTTTGAAATGAATTGAAATTAGAATGTAACGTGATGATTGAATATAATGGAAATGTTGAATTTGGTTAAAGTAAAAAGGTTT
TARGET=692,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1121
PRIMER_LEFT_PENALTY=0.111365
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=CACAGTTGGTGAAGTGTGTCCT
PRIMER_RIGHT_SEQUENCE=TCCAAGCTAACCCACTGAATTT
PRIMER_LEFT=631,22
PRIMER_RIGHT=820,22
PRIMER_LEFT_TM=60.111
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=0.1121
PRIMER_LEFT_1_PENALTY=0.111365
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=CACAGTTGGTGAAGTGTGTCCT
PRIMER_RIGHT_1_SEQUENCE=CAAGCTAACCCACTGAATTTCC
PRIMER_LEFT_1=631,22
PRIMER_RIGHT_1=818,22
PRIMER_LEFT_1_TM=60.111
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=188
PRIMER_PAIR_PENALTY_2=0.1121
PRIMER_LEFT_2_PENALTY=0.111365
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=CACAGTTGGTGAAGTGTGTCCT
PRIMER_RIGHT_2_SEQUENCE=AAGCTAACCCACTGAATTTCCA
PRIMER_LEFT_2=631,22
PRIMER_RIGHT_2=817,22
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=0.1234
PRIMER_LEFT_3_PENALTY=0.122657
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=GATTGGAGCTAGGATACAACGC
PRIMER_RIGHT_3_SEQUENCE=AAGCTAACCCACTGAATTTCCA
PRIMER_LEFT_3=544,22
PRIMER_RIGHT_3=817,22
PRIMER_LEFT_3_TM=60.123
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.1234
PRIMER_LEFT_4_PENALTY=0.122657
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=GATTGGAGCTAGGATACAACGC
PRIMER_RIGHT_4_SEQUENCE=CAAGCTAACCCACTGAATTTCC
PRIMER_LEFT_4=544,22
PRIMER_RIGHT_4=818,22
PRIMER_LEFT_4_TM=60.123
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_04898 Ps_contig_mira-and-tgicl-ass_franssen_35134
SEQUENCE=TTTTAGTATTCATTAAATTGAATCAAAACATAAATCATGAGGCGGCAAATGGTYGTGTTCAAATGAAGAGGACAAACCTCCCACTAACGAATCACACAATAGGGTCTTTATAAGATATTTTAAGAATTAACCTAAGTCGAAATGGCCATATAAACTTTCTGGATAAACCGAATCTGTAATTTCAATACATGAGAAACCTAATGTATCTATCTATCTATTTGTAGCATATTTATATCCAGCACAACTGAGATGAACTTCAGAATTAAACAGAAGAAGTGACCATATAAACACTTTAGATAAACCGTATCTGTGTAATTCCAAATCATGAGAAATTGGCAATAATCCAAGTCAAACAGGAACTGGAATGCCCTTCTTAGCCATACTATCCAAAACATCGTTGAGAGCTTGACATAGGCCAACAATCTGCTGGTCCCATCTCTGCAACTCTTCAGTGTCATCGTCAAAATGTATGATAGCTTCAACCTGATCGATAGATCCCTTCATCCTATCCTCATAAATCATCCGTGAAGCTATCTTCTCAGCCTTGGGAGGTGGGATTCCCAGTAGTGTGCCTAACTCGTCAAAGCTGATATTAGTATAAAGTTTGCTTGCACTGAGAAGATTATGCTCAATCATTGCTCGGTCCAGCACAGTGAAATTGTCTGGAAGAAGGGCTTTCTGATGTGGTTTCAATTCTTCTGCAAAAGCATCAATTTCAGGCCTTCGAAGAATTCTTTCCAAATACACCTTTTGCAGTATGGGATAGATCTTCAACTTTGAACATCGCTCATCCTTGTACAATGTGGCAAGAACACGGGAACGTTGAGGCCCTGCGCCTGCCAATATTGTGCATGTAACGGCAGCAGATAAAGCTTGCCCAAGTGCTTCCTCATTAATTTCCTCGTCTCCTATTTGCCTTTTCTCAATTTGAGAAATGTCATAATATCGTAAGGCAGCCTCCAAGAACTTCCTCTTCAGATCTAAGATCCTTGCATAGCAAACCTTGTACTTCAAATTCAGCACTTCATGCTGGCTATTGCTAACCAAGAATGATGCTTTATTAATGAAAGCTTCAGCATTAACAGCATCGTCATCCTCAAGATATAAACAGGCAATTTGGACACACTTTGATAATCTGAATGTATCATCAATTACCCTCATTCCTGAATCTAGGTCAATACCACTGAGCATCTGGGCGGCTTTTGACCATTGCTCCTCAGATTCATAAAGCTCAGCCAATTTCTCTCTAATAACCAAAACCTGTTCTTCAAAGGAAACAACTCGAGGCTGAATCTGAGCAAGTATGAAATGAGCAATCTCTTTCTGCGTCTCCGGCGTCAATCTTCCCAACTCTTCAGCAAAACCCTGCAGAAGCTGCCGCGACACTACTAAAGGAACATCATCTGATAATATGTGATCTANGAACTTTCTAGCTTGACCGATAT
TARGET=204,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04899 Ps_contig_mira-and-tgicl-ass_franssen_35137
SEQUENCE=TTTTTGGCATAGTTATAGGAAAGTTCATTGATATTGTTTTAACTAATTTTAGTTGTTTATTCTTAGTGACAATGTATTTTGACCACTTCTCGACCTGTGTTAAATTTCCAGAGATTATGTACTAGTATATAGTATGATACTATTACTAGTATCTGTCATGGTACACATTGTGCAGAATCAGTTGAAATGCTGGAAACAACAAAAAGCTCCAACTACTCATCAATTGAAAAAGTCACTCCATCATGCTTCTTGAAGTAGTGTGTGGTATGATCAACCACATTTGTATTGCTAAGTTCTAAATTATAAAAGTCCATTGCATCAGACGTAAATAGAGGACCGGCATGCCATGTCCCACGGTTAAGCTTGAGAAATTTGGGACCTGAAACCTTGAAAACTTGGACATCCTCAATGGCAGGAGGCGCGTAAAAATGACCACCGCGTGACTGCACGATTGTCTTGCCTGTGTTATCCTTGATTTCATTTGAATCAACAATAGATGGCTTAGCCACTCCAAGATACCAAACATTACCACCAATGGACCCAAGGCACTGAGTCACACATGCATGATGTGTGATACTAGAAAACTTAAGTGGCCGGTTCTCAAGATGCATAATGTAGAACCTGGGAATTCCTTTAGTGAGGTCAAGTTGAGCATCATGGGGACCGAAACCCTCGCCGTCCGGTGAAGCCTCAATGACCTGGCCGTAGTCTTGAAAGGTTGACGGAGTTGCTTCGATTGGTTTCACGGTAACCACCGTCACACTCTCCATTTCCGATTAGATTATTATTATTATTATGCTATCACAATTTTGGTGCCACAAATTTAATAGTAAAAATAAGTAAAATACAAATCACGCTAAAATCAAGCAACTATCATGAGAAATATCCTCTATATGTGATAGGATTATAATTAGTTATCCACAATAGATCGCCTTCGATAGAGATATAGATTTAAAATTTAAATCAGATGCAAGTT
TARGET=781,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2940
PRIMER_LEFT_PENALTY=0.171757
PRIMER_RIGHT_PENALTY=1.122288
PRIMER_LEFT_SEQUENCE=GCCGTAGTCTTGAAAGGTTGAC
PRIMER_RIGHT_SEQUENCE=CTATCGAAGGCGATCTATTGTG
PRIMER_LEFT=701,22
PRIMER_RIGHT=940,22
PRIMER_LEFT_TM=60.172
PRIMER_RIGHT_TM=58.878
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=1.4621
PRIMER_LEFT_1_PENALTY=0.339815
PRIMER_RIGHT_1_PENALTY=1.122288
PRIMER_LEFT_1_SEQUENCE=GTCTTGAAAGGTTGACGGAGTT
PRIMER_RIGHT_1_SEQUENCE=CTATCGAAGGCGATCTATTGTG
PRIMER_LEFT_1=707,22
PRIMER_RIGHT_1=940,22
PRIMER_LEFT_1_TM=59.660
PRIMER_RIGHT_1_TM=58.878
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=1.4701
PRIMER_LEFT_2_PENALTY=0.171757
PRIMER_RIGHT_2_PENALTY=1.298349
PRIMER_LEFT_2_SEQUENCE=GCCGTAGTCTTGAAAGGTTGAC
PRIMER_RIGHT_2_SEQUENCE=TATCGAAGGCGATCTATTGTGG
PRIMER_LEFT_2=701,22
PRIMER_RIGHT_2=939,22
PRIMER_LEFT_2_TM=60.172
PRIMER_RIGHT_2_TM=61.298
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=1.4701
PRIMER_LEFT_3_PENALTY=0.171757
PRIMER_RIGHT_3_PENALTY=1.298349
PRIMER_LEFT_3_SEQUENCE=GCCGTAGTCTTGAAAGGTTGAC
PRIMER_RIGHT_3_SEQUENCE=CGAAGGCGATCTATTGTGGATA
PRIMER_LEFT_3=701,22
PRIMER_RIGHT_3=936,22
PRIMER_LEFT_3_TM=60.172
PRIMER_RIGHT_3_TM=61.298
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=1.4919
PRIMER_LEFT_4_PENALTY=0.369628
PRIMER_RIGHT_4_PENALTY=1.122288
PRIMER_LEFT_4_SEQUENCE=GTCACACTCTCCATTTCCGATT
PRIMER_RIGHT_4_SEQUENCE=CTATCGAAGGCGATCTATTGTG
PRIMER_LEFT_4=756,22
PRIMER_RIGHT_4=940,22
PRIMER_LEFT_4_TM=60.370
PRIMER_RIGHT_4_TM=58.878
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_04900 Ps_contig_mira-and-tgicl-ass_franssen_35156
SEQUENCE=AAAACGAAATGGACCATAAAAYTAACACCTCAAGGCAAATATACAGCCATTTGTATTGCCAAAACAAAAGCCCCTGATTTGTTCAAATATCTCAGCTAATTAGCTAAATCAGGGATTGAAAGAAACAAAACTAAAGGACTCCTTYTCAGTCAACTGTATTTCAGTGACGTGGTTGATAAATAAATAACTCACTAAGATAACTGTTAGTATTAATAAATATATGACGGATTTCTGCGTCTCTACACATTCTTCCACGCATACAAAATAAGCGAAAAACCAAGACCCGGAAATTTGGTCTCGGAAAACCTCTCAGAGAAAGACCACTGAAGCAGACCAGCATCATCAGAAGAAAGGGGTTGTCTAGTCCACAACTTGTCCGCGGAGAGGCACGTCCTGAACATAAAAACAGCATCTGCAAGAGAAGCGCCAAAGAGCCAATTATGAACATCCCAAAAAACTTCCACCGCAACGCCATCAACAAGGATTATATGATTCCCCCTAAACTTCCACCTCAACCGATTCACTTGCATCACAATTTTGCTGTCTATGCGAATTATAAGCGACGGATCACTAACACCACTTGTGGTATCACATTCAATCACAAGATCATGAATTTGACCATTATCAACAAAACACAGTCTTGGTGCCATACAACTTCTAACCAAAACATTCTTCTTTCTTGGCTAACTAAAACANCTCCATCGGAACCG
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04901 Ps_contig_mira-and-tgicl-ass_franssen_35159
SEQUENCE=CGAAAGAAAAATAAATAAATAANTGAAGAATNTATTATTTGTTATTACACAAAACACATCCATGATAATTGATATCATATCTCTCACTCATATGTTAAAAATCAAACATAGCACAACAATATAGTTAAATACGAATTCTAAAGTAAGAAAACAAATTTCAAAAATCAAAGAAATCACAACCATGAATCATAGTTGAAACCTCGTCGATGCACATCTTCAAACACTTGAGATCGATCTCTATGTTTAGGAAGACGAGGAAGCTCAGATAGTTTAACATAACTATCATCCTTTACATCCATTCCACTCCTACATGAAAAAATACAATAGCTTCTCCTTAAACTTACACAATTATTAAATCTAACACCTTTTTTTGTTCTTGCTTTGAAAACTTAGCAACTTTTCTCACTTTTAGTACATTCCTTGTAC
TARGET=9,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04902 Ps_contig_mira-and-tgicl-ass_franssen_35166
SEQUENCE=TTNAGACTCGTCTTCCTTACTGAGCGAGTGTGGATCTAGCGAAGTAGAAGAAGATGCAGTGGTACTTGGTCGCTACGCTTCTCACCGTTCTCACCAGTTCTCAGGGAATACTAACAACCCTATCGCAAACTAATGGAACCTATAAGTATGATTATGCAACTGTGCCATTTCTAGCTGAGGTTTTTAAGCTTGCTGTGTCAAGTTTGTTACTTTGGAAGGAATGTCAAAAATCACCTCTTCCAAAGATGACAACGGAGTGGAAAACAGTTTCTTTGTATCCAATTCCTTCTGTTATATATCTCATTCATAATAATGTTCAGTTTGCTACTCTCACATTTGTCGACACTTCCACTTATCAGATAATGGGTAATTTGAAGATTGTTACCACTGGGATATTATTCAGGCTTTTCTTAGGGAGGAAGCTTTCCAACTTGCAATGGATTGCCATTATGCTGTTGGCCGTTGGAACAACCACAAGTCAGGTCAAGGGCTGTGGAGAGGCTTCCTGTGACTCCCTATTCTCAGCACCAATTCAGGGTTACATGTTAGGAGTTCTATCTGCTTGTCTCTCAGCATTAGCTGGAATTTATACAGAGTTTTTGATGAAGAAAAACAACGATACCTTATACTGGCAGAATATACAGTTGTACACGTTTGGTTCAATTTTCAATTTGGCAAAGCTTATTGTGGAYGATTTCAGAGGTGGCTTTGAAAATGGGCCCTGGTGGCATCGCATTTTTAATGGATACACAGTCACCACTTGGTTGGTGGTATTAAATCTAGGGTCTACCGGTCTTTTGGTTTCATGGTTAATGAAACATGCTGATAATATTGTTAAGGTCTATTCAACATCCATGGCAATGCTGCTTACAATGATTCTATCAACATTCCTCTTCAGTTTCAAACCTACATTGCAGCTATTCTTGGGAATTATTATTTGTATGATGTCTTTACACATGTATTTTGCCCCACCAAATATGCTCTTAGATATGCCATTAACAGTTAAACCTGATGAAGAAGAGAGGCTCATTGAAGTTTCTGTTGATCGGAGAACACATTCATGATGTATATTGACATCTGAATACAATGTTTTGGTAATTTATTTCAATTATAATTTATTTTATTTTATCAATTCATCAAAAAAATACATTACCATATCATATCTAACATTTCTAGTTAGATGATACGTGTAGCTTTAAGAAAGTATTTATTCAATGTAAATTTTGATTAAACGAGTAACATTGTTGCCTTGTGACATTAGTGGTCCTAGGTTAAAAGTGGAAATAGTCTTTCTTTTGCCAATGGTAGTGCTGTGTACTTACCCTACAAAACTCCACATAGTGGCATCTAATGAAATTGGTCACCYTTCT
TARGET=1115,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04903 Ps_contig_mira-and-tgicl-ass_franssen_35180
SEQUENCE=GTCGGGGTACCAACATTNATTCTCACTGAAAGAGACAATCAAAACACAACAGCAAATAGAGAGAGAAGAGAAGAGAAAATGGAGAAAAAAGTTGTGGCTGTGTGCTGCGTTGTGGGCTTCTTAGGGCTCTTATCAGCTGCAACTGCCTTTGCTGCTGAAGCAACAAGGATTAAGGGCTATCAAATTCAGTTTGTCTCGCCAAATCAGTGCACGTATCCTCGAAGTCCAGCTCTACCTCTTGGTTTAACTGCGGCGCTGTCTCTTATGATTTCTCAAATTCTCATAAATCTTTCAACTGGATGCATTTGTTGCAGAAGAAATTTGCGAATCCCTGATGCCAGTTGGACGGTGGCACTGGCCTGCTTTGTTTTATCCTGGTTCACATATTTAATTGCATTTCTCCTGTTGCTAACCGGTGCTGCACTGAACGATCAGCGTGGCGAAGAGAGTCTATACTTTGGAACCTACTACTGTTATGTTGTCAAACCTGGAGTGTTTGCTGGTGGCGCAATGTTATCTCTTGCAAGTGTTGCGTTTGGAATTATCTATTACATTACCATAATGGAGGGAAAGAATGCTAGCAATGCTTTTGGTAATTCCTCAAACCCTAATCAAGGGAATATAGCCATGGGACAACCACAGATTCCAACTCAAACCAGTCAAGACCCGGTATTCGTGCACGAAGACACGTACGTCAGGCGACAGTTCACATGATTGATGAACAACTGTCTGTCATTAAAAGCTCAGTGCTGTGTTACTCAGTTTCTGAGGCTTGAAATTTGGAGATTGCTTTAGTCAAAAACCTGGTTTATGTGTATTCCTAGGATGTAGTGTTCTATTAGTTTTATGTACATGTTTCACCCAAAAAATGGTTTGTGAGATTATGAAATTTATGGTGTTTATAATTGAATGATATATACTAGTAAGTTTTTAAATCATGGTCATGTTGCAATGACTTNAAATTCCTTGATTTTGCTACCTACATTATCGATGGAATGCCACCTTCGGAATTGTATTTGATGT
TARGET=62,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7941
PRIMER_LEFT_PENALTY=0.679382
PRIMER_RIGHT_PENALTY=0.114715
PRIMER_LEFT_SEQUENCE=GGGGTACCAACATTNATTCTCA
PRIMER_RIGHT_SEQUENCE=ATAAGAGCCCTAAGAAGCCCAC
PRIMER_LEFT=3,22
PRIMER_RIGHT=132,22
PRIMER_LEFT_TM=59.321
PRIMER_RIGHT_TM=60.115
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=0.7959
PRIMER_LEFT_1_PENALTY=0.679382
PRIMER_RIGHT_1_PENALTY=0.116503
PRIMER_LEFT_1_SEQUENCE=GGGGTACCAACATTNATTCTCA
PRIMER_RIGHT_1_SEQUENCE=AGAAATCATAAGAGACAGCGCC
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=59.321
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.8016
PRIMER_LEFT_2_PENALTY=0.679382
PRIMER_RIGHT_2_PENALTY=0.122257
PRIMER_LEFT_2_SEQUENCE=GGGGTACCAACATTNATTCTCA
PRIMER_RIGHT_2_SEQUENCE=CCCTTAATCCTTGTTGCTTCAG
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=175,22
PRIMER_LEFT_2_TM=59.321
PRIMER_RIGHT_2_TM=60.122
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.75
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.8154
PRIMER_LEFT_3_PENALTY=0.679382
PRIMER_RIGHT_3_PENALTY=0.135999
PRIMER_LEFT_3_SEQUENCE=GGGGTACCAACATTNATTCTCA
PRIMER_RIGHT_3_SEQUENCE=GCAGCTGATAAGAGCCCTAAGA
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=139,22
PRIMER_LEFT_3_TM=59.321
PRIMER_RIGHT_3_TM=60.136
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=137
PRIMER_PAIR_PENALTY_4=0.8545
PRIMER_LEFT_4_PENALTY=0.679382
PRIMER_RIGHT_4_PENALTY=0.175100
PRIMER_LEFT_4_SEQUENCE=GGGGTACCAACATTNATTCTCA
PRIMER_RIGHT_4_SEQUENCE=CCGCAGTTAAACCAAGAGGTAG
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=59.321
PRIMER_RIGHT_4_TM=60.175
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=250
=
PRIMER_SEQUENCE_ID=SSR_04904 Ps_contig_mira-and-tgicl-ass_franssen_35185
SEQUENCE=CCTTTTTCGGTTTTGGGTCCTTTCGGGTAAGTTAATTTTACCCTTTTTTGGTCCGTTCCTTTTTCCTTTGGTCCACCAAAAATTTTACCCTTCCTATTTTTTGGTCACAAAGGTCCTAATTGGAAAGTTCCATTAGGAAGAAGAGATGGTTTAACAGCAAATCAATCACTTGCTAACACAAATCTTCCAGCTCCTTTCAACACATTAGATGAACTCAAAGCTGCTTTTGCTAAACAAGGCCTCACCCCTACTGATCTAGTTGCTCTTTTCCGGTAGCTCACACATTCGGAAGATCTCACTGTTCCTTATTCGTCGATAGGTTATACAATTTCAGCAATACCGGAAAGCCTGATCCATCTCNCAACACAACTTACTTACAAGAGTTACGACAAAACATCGCCCGTAAGGGTGGAAGTGGAACAAACCTAGCCTAATTTCGACCCTACTACTCCGGATAGTATTTGAACAAGAACTACTACTACTAATCTTACGTAAGGTTTAAAAGGGTTTGTACC
TARGET=472,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.4260
PRIMER_LEFT_PENALTY=0.039879
PRIMER_RIGHT_PENALTY=3.386086
PRIMER_LEFT_SEQUENCE=GTGGAAGTGGAACAAACCTAGC
PRIMER_RIGHT_SEQUENCE=GGTACAAACCCTTTTAAACCTTACG
PRIMER_LEFT=408,22
PRIMER_RIGHT=514,25
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=60.386
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=3.4802
PRIMER_LEFT_1_PENALTY=0.094139
PRIMER_RIGHT_1_PENALTY=3.386086
PRIMER_LEFT_1_SEQUENCE=TGATCCATCTCNCAACACAACT
PRIMER_RIGHT_1_SEQUENCE=GGTACAAACCCTTTTAAACCTTACG
PRIMER_LEFT_1=349,22
PRIMER_RIGHT_1=514,25
PRIMER_LEFT_1_TM=59.906
PRIMER_RIGHT_1_TM=60.386
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=166
PRIMER_PAIR_PENALTY_2=3.4802
PRIMER_LEFT_2_PENALTY=0.094139
PRIMER_RIGHT_2_PENALTY=3.386086
PRIMER_LEFT_2_SEQUENCE=CTGATCCATCTCNCAACACAAC
PRIMER_RIGHT_2_SEQUENCE=GGTACAAACCCTTTTAAACCTTACG
PRIMER_LEFT_2=348,22
PRIMER_RIGHT_2=514,25
PRIMER_LEFT_2_TM=59.906
PRIMER_RIGHT_2_TM=60.386
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.7000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=3.6328
PRIMER_LEFT_3_PENALTY=0.246726
PRIMER_RIGHT_3_PENALTY=3.386086
PRIMER_LEFT_3_SEQUENCE=TCGGAAGATCTCACTGTTCCTT
PRIMER_RIGHT_3_SEQUENCE=GGTACAAACCCTTTTAAACCTTACG
PRIMER_LEFT_3=285,22
PRIMER_RIGHT_3=514,25
PRIMER_LEFT_3_TM=60.247
PRIMER_RIGHT_3_TM=60.386
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=3.6328
PRIMER_LEFT_4_PENALTY=0.246726
PRIMER_RIGHT_4_PENALTY=3.386086
PRIMER_LEFT_4_SEQUENCE=TTCGGAAGATCTCACTGTTCCT
PRIMER_RIGHT_4_SEQUENCE=GGTACAAACCCTTTTAAACCTTACG
PRIMER_LEFT_4=284,22
PRIMER_RIGHT_4=514,25
PRIMER_LEFT_4_TM=60.247
PRIMER_RIGHT_4_TM=60.386
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_04905 Ps_contig_mira-and-tgicl-ass_franssen_35188
SEQUENCE=AACTTTNAACTACACAGAAAGAGGGTANTATNATTCTGAATGAAAAGTGCATCCATTGTAACATCATGCCAATAGTTACCATATCTGACATAAGCCAAAGTATAGTATACTAAACAACCAATAGACCAATATATAAATAAATAAAAAATAACAAAAAAAACCAATACCGCGTCCCCAAATATAATTTGAATACAACAAGAATTAACCAGTTCTTATTCTGTCGAGTCGGTCAACACTCTTCCTTCCTTTCTCTAATTGATGATCAATGCTTTTTCGAGACTTCTCATGACAGTCAACACTTCTTCGAGATCTCTCTTTATGATCAAACGTCATTCTTAATTTCTCCAACATATCTCCGCCATTACTAGAAGACAAGTTTTTGACCTTCTCTGGTTTGTCTGTGCTTTTCCTTGGCTTTTCTCTACGGTCGGTGCTCTTTCTACTCACTTCGAAAATATCCGTACTCTTTCGAGGCTGCTCAAACTGCTGGTCTGTACTCCTTCGGAAACTGTATCGTTGCGACGGAGACTTCTCAATTGTTGTTATGAACTTCTTCAGATGCCTAATGTACTCTGGAAAGAGCTCCAAATCACAGTGGTTACCTCCTTTGAGCCACAACGGTTCATATTTCTCTTTACAAAGTTCCCAGAGTTGCTTACCGTGGGAGCAATCTACAACTTCATCTGAAGTACCATGAACTATGAGAACTGGACAATTAACTAGTGGAATTTTGTCGATATTCTTAAATATGTCAAACCAGTATGAACGTTTTACTGGATACATGACCCTTAAGCCAGAAAGTATTGGACTGTGCAAAACAACAGCTCGTAATTGAGGCAACCGAGCCGCAATTTCCAAAGTCGGGCCACTTCCAACAGATTGTCCGTAAAGGATTACATCTTCCTGCTTAGCACCATAACTTTCTTCTAGACACTTGTATACAGCCTCAATATCTGAATATGTATTCTGCTCACTTGGCTTTCCCGATGATTGCCCATACCCAGAATAATCATACCCCATGAGATTAACGCGCAAGTGGATGCTAAGCTCAATGAAAAGCTCATACATCTGACCAAGATCAGCAGCGTTTCCATGAGAGTAAAGAAGAGTAGAAGTAGCCATTGGGTGACGAACATAAACCGCCACAATCTCCGTTCCACGGCGCGTAGGAAGCTTAAGAATCTCAACGTTTTCACGGTGAGGGAAAGGAGTGATGAGAAGAAGAGAAGTTAGTTCGTTCTTTGACAAGATTGTATGAGTGGGTGGGTCGTTTGGGTTAGGGAAAAGGAATGACTAAATTTTGCTGCCATTGATAGAAGTAA
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6598
PRIMER_LEFT_PENALTY=1.535132
PRIMER_RIGHT_PENALTY=0.124655
PRIMER_LEFT_SEQUENCE=CATCCATTGTAACATCATGCCA
PRIMER_RIGHT_SEQUENCE=TGACCGACTCGACAGAATAAGA
PRIMER_LEFT=49,22
PRIMER_RIGHT=231,22
PRIMER_LEFT_TM=61.535
PRIMER_RIGHT_TM=59.875
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=1.7507
PRIMER_LEFT_1_PENALTY=1.535132
PRIMER_RIGHT_1_PENALTY=0.215550
PRIMER_LEFT_1_SEQUENCE=CATCCATTGTAACATCATGCCA
PRIMER_RIGHT_1_SEQUENCE=GAAGGAAGAGTGTTGACCGACT
PRIMER_LEFT_1=49,22
PRIMER_RIGHT_1=244,22
PRIMER_LEFT_1_TM=61.535
PRIMER_RIGHT_1_TM=59.784
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=1.9033
PRIMER_LEFT_2_PENALTY=1.535132
PRIMER_RIGHT_2_PENALTY=0.368190
PRIMER_LEFT_2_SEQUENCE=CATCCATTGTAACATCATGCCA
PRIMER_RIGHT_2_SEQUENCE=CATGAGAAGTCTCGAAAAAGCA
PRIMER_LEFT_2=49,22
PRIMER_RIGHT_2=287,22
PRIMER_LEFT_2_TM=61.535
PRIMER_RIGHT_2_TM=59.632
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=2.1565
PRIMER_LEFT_3_PENALTY=1.535132
PRIMER_RIGHT_3_PENALTY=0.621340
PRIMER_LEFT_3_SEQUENCE=CATCCATTGTAACATCATGCCA
PRIMER_RIGHT_3_SEQUENCE=ATTATATTTGGGGACGCGGTAT
PRIMER_LEFT_3=49,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=61.535
PRIMER_RIGHT_3_TM=60.621
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=6.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=2.1570
PRIMER_LEFT_4_PENALTY=1.535132
PRIMER_RIGHT_4_PENALTY=0.621889
PRIMER_LEFT_4_SEQUENCE=CATCCATTGTAACATCATGCCA
PRIMER_RIGHT_4_SEQUENCE=TTGACCGACTCGACAGAATAAG
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=232,22
PRIMER_LEFT_4_TM=61.535
PRIMER_RIGHT_4_TM=59.378
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_04906 Ps_contig_mira-and-tgicl-ass_franssen_35198
SEQUENCE=CAACAACATCACACNAATGGAAATACGTTTCAATCATTACCACGAAGTTGAATTGTTTATTGAATCGGAGCTTGATGGCGTCGATTATTGTTGTGCTGCTGAAATGGAAAAGTTATGTGGGTGATGCAAGCAACTGAGTTGTTGTTGTTGTTGTTCTGTTTCACCGGAGAAGAATACAATGATGATGGTTGCTTGCTACATTCGATGTTCGAAGCGAAAAGGAAAGAGCGCTGATTTGTGCAAGATTGTACGTGTTGTCGTATAATCATTGTCAGCGGAGCTAAAAGGTGGCGTGTGGCGTGTGGTTCGAAGGGTCATGTTGTTCGATGGGTGGAGGTTTTGTTGGAGTTTCACAGTATGGAATGAGAAGTTATGGATGTGAGGAGATTGAGGGTTGCTTGAAGTTAAAAGAGGAAGGGAAGCTTGTCGGATCTGGAACGGAGGTTTGACGGTTGTTGGTATGCGATCTCCGATTTATGGGTTGTAAGGCAGAGAGTGGAGTGTTGTAAGGTGGGTTTTGAAGTTTTTCGGAAAGAAGAAGATAGAAGATGGAGTTTGGGTGGTTTTATGGTTGTTGATGGAGGTTGGGTGGTTGTCACTGACGATGATAAATGTGGAGGAGATAGAGGCACAATGTTGGTGAATGGTGTTTTTTTAAAAAGGTTGAGTGTGTGGAAATGCTATGGAGGCATGAGTGTGTGACGATAGTTATAAGAAGATGAAAATAAGTATATATGATGAAGGTAAGAATGCAGAGACTATGAGGTCTTTGTTTTTTTTCTGAGAGAGGAGAGTATTCTTTTTTTCAGAGAGTCTCTGCATCCATTTTGGTTGTCGTNGGCAAAGGGAATAAAAACTTCATCTTACGTAGCCAT
TARGET=138,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3902
PRIMER_LEFT_PENALTY=0.275749
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=TGGAAAAGTTATGTGGGTGATG
PRIMER_RIGHT_SEQUENCE=GTGAAACTCCAACAAAACCTCC
PRIMER_LEFT=104,22
PRIMER_RIGHT=353,22
PRIMER_LEFT_TM=59.724
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=0.4012
PRIMER_LEFT_1_PENALTY=0.275749
PRIMER_RIGHT_1_PENALTY=0.125411
PRIMER_LEFT_1_SEQUENCE=TGGAAAAGTTATGTGGGTGATG
PRIMER_RIGHT_1_SEQUENCE=TTAGCTCCGCTGACAATGATTA
PRIMER_LEFT_1=104,22
PRIMER_RIGHT_1=283,22
PRIMER_LEFT_1_TM=59.724
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.5400
PRIMER_LEFT_2_PENALTY=0.275749
PRIMER_RIGHT_2_PENALTY=0.264300
PRIMER_LEFT_2_SEQUENCE=TGGAAAAGTTATGTGGGTGATG
PRIMER_RIGHT_2_SEQUENCE=TTCGAACATCGAATGTAGCAAG
PRIMER_LEFT_2=104,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=59.724
PRIMER_RIGHT_2_TM=60.264
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=0.5477
PRIMER_LEFT_3_PENALTY=0.275749
PRIMER_RIGHT_3_PENALTY=0.271999
PRIMER_LEFT_3_SEQUENCE=TGGAAAAGTTATGTGGGTGATG
PRIMER_RIGHT_3_SEQUENCE=GAACATCGAATGTAGCAAGCAA
PRIMER_LEFT_3=104,22
PRIMER_RIGHT_3=209,22
PRIMER_LEFT_3_TM=59.724
PRIMER_RIGHT_3_TM=60.272
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.5892
PRIMER_LEFT_4_PENALTY=0.486257
PRIMER_RIGHT_4_PENALTY=0.102945
PRIMER_LEFT_4_SEQUENCE=CGTTTCAATCATTACCACGAAG
PRIMER_RIGHT_4_SEQUENCE=ACGACAACACGTACAATCTTGC
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=260,22
PRIMER_LEFT_4_TM=59.514
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_04907 Ps_contig_mira-and-tgicl-ass_franssen_35209
SEQUENCE=GGGGGAACACGTGCAAACCATGTTTCACGTTTTCCACATACATCCCTACTCAGTCAGACTTCCAATCTTATCTCTCTCCCTTCACCGCGCTGGAGCTTACCATCGACGGTGGTGGAGCATCAGATATCAAAATTACATATGCCAAAATGTTCTATTTCTCAAGCTCTACCAAAAACTACCATCAATTTCACCTGAATCTCCATAAATTCTATAGATCGAGCAAGAACAAGCAAAGAACCCTAGATACTAAATTAGAAATTAAATGATGGATTGGAGCAATCAAAGTCCCTGATGTTAGGGATTTGATTCCCCGACTCTCGCTCTACATGATGGTAACTCGTATGCATCAAATGATAGAGAAAATCAAAACTACCTGCTAGAGAAAACGGCCGAGAACGAAGCAAAACAACGTCTGAACAAGTTTAGGATGTTGCATTGCAGTTAGGTGGCAGTGTATTGGATAACTTGGTGAACCAATTTAGACTCGAGCTGCAGATTGAAATGTTTTGGCATTGCAGGTGGCTTGCAACAAGTAATTTGTTGATGATGATGACCTAATATTTCAGGTGACATGTATGGAAATACATGCAATGCAATGGTTGGTTAAAGTGCAAACAGGTTTTGCAGGTTATTCATATGCTCATGCCATGGTGTGCAAAAGGTATGTTCATGAAATGTGAATTTGTAGCACGCATGGTTAGGGATTCTATGTTATGTTTGGTTGGTTTTGTATGATAATTGGATGATGTGTGAATGCTTGCTTGTGTATTACATGATGTATGGCTACATGTTTGTGAAATTGGTCACGGTGACAAGGCAAATAAGGCATGACCACCCAAGTTTAATGCAAAAGTAAAGTTTCTTATTCATGAAGAAACATGATGGTGGAAGCTCATGAAATAATTTGGATGTAATTAGCATTAAGGTTTAGGAGATGAAAAAATGTGATAGTTAACTTTGGGCAATTGGTTGAACAAAAAAGTACAACTAGTTGACAAAGNTTCAACTTTTGCAAAAATGCTATTTTTTGGTATTTTATTTGTAATTGACAATAGTAAAATGTTAGTATGAATGAATGAA
TARGET=542,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1078
PRIMER_LEFT_PENALTY=0.080887
PRIMER_RIGHT_PENALTY=0.026872
PRIMER_LEFT_SEQUENCE=AACCAATTTAGACTCGAGCTGC
PRIMER_RIGHT_SEQUENCE=TAACCATGCGTGCTACAAATTC
PRIMER_LEFT=471,22
PRIMER_RIGHT=699,22
PRIMER_LEFT_TM=59.919
PRIMER_RIGHT_TM=60.027
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.1486
PRIMER_LEFT_1_PENALTY=0.080887
PRIMER_RIGHT_1_PENALTY=0.067678
PRIMER_LEFT_1_SEQUENCE=AACCAATTTAGACTCGAGCTGC
PRIMER_RIGHT_1_SEQUENCE=CTGTTTGCACTTTAACCAACCA
PRIMER_LEFT_1=471,22
PRIMER_RIGHT_1=617,22
PRIMER_LEFT_1_TM=59.919
PRIMER_RIGHT_1_TM=60.068
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.2282
PRIMER_LEFT_2_PENALTY=0.080887
PRIMER_RIGHT_2_PENALTY=0.147302
PRIMER_LEFT_2_SEQUENCE=AACCAATTTAGACTCGAGCTGC
PRIMER_RIGHT_2_SEQUENCE=AAGCAAGCATTCACACATCATC
PRIMER_LEFT_2=471,22
PRIMER_RIGHT_2=762,22
PRIMER_LEFT_2_TM=59.919
PRIMER_RIGHT_2_TM=60.147
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=292
PRIMER_PAIR_PENALTY_3=0.2585
PRIMER_LEFT_3_PENALTY=0.080887
PRIMER_RIGHT_3_PENALTY=0.177596
PRIMER_LEFT_3_SEQUENCE=AACCAATTTAGACTCGAGCTGC
PRIMER_RIGHT_3_SEQUENCE=TGGCATGAGCATATGAATAACC
PRIMER_LEFT_3=471,22
PRIMER_RIGHT_3=647,22
PRIMER_LEFT_3_TM=59.919
PRIMER_RIGHT_3_TM=59.822
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=177
PRIMER_PAIR_PENALTY_4=0.2769
PRIMER_LEFT_4_PENALTY=0.209271
PRIMER_RIGHT_4_PENALTY=0.067678
PRIMER_LEFT_4_SEQUENCE=CGCTCTACATGATGGTAACTCG
PRIMER_RIGHT_4_SEQUENCE=CTGTTTGCACTTTAACCAACCA
PRIMER_LEFT_4=318,22
PRIMER_RIGHT_4=617,22
PRIMER_LEFT_4_TM=59.791
PRIMER_RIGHT_4_TM=60.068
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=300
=
PRIMER_SEQUENCE_ID=SSR_04908 Ps_contig_mira-and-tgicl-ass_franssen_35209
SEQUENCE=GGGGGAACACGTGCAAACCATGTTTCACGTTTTCCACATACATCCCTACTCAGTCAGACTTCCAATCTTATCTCTCTCCCTTCACCGCGCTGGAGCTTACCATCGACGGTGGTGGAGCATCAGATATCAAAATTACATATGCCAAAATGTTCTATTTCTCAAGCTCTACCAAAAACTACCATCAATTTCACCTGAATCTCCATAAATTCTATAGATCGAGCAAGAACAAGCAAAGAACCCTAGATACTAAATTAGAAATTAAATGATGGATTGGAGCAATCAAAGTCCCTGATGTTAGGGATTTGATTCCCCGACTCTCGCTCTACATGATGGTAACTCGTATGCATCAAATGATAGAGAAAATCAAAACTACCTGCTAGAGAAAACGGCCGAGAACGAAGCAAAACAACGTCTGAACAAGTTTAGGATGTTGCATTGCAGTTAGGTGGCAGTGTATTGGATAACTTGGTGAACCAATTTAGACTCGAGCTGCAGATTGAAATGTTTTGGCATTGCAGGTGGCTTGCAACAAGTAATTTGTTGATGATGATGACCTAATATTTCAGGTGACATGTATGGAAATACATGCAATGCAATGGTTGGTTAAAGTGCAAACAGGTTTTGCAGGTTATTCATATGCTCATGCCATGGTGTGCAAAAGGTATGTTCATGAAATGTGAATTTGTAGCACGCATGGTTAGGGATTCTATGTTATGTTTGGTTGGTTTTGTATGATAATTGGATGATGTGTGAATGCTTGCTTGTGTATTACATGATGTATGGCTACATGTTTGTGAAATTGGTCACGGTGACAAGGCAAATAAGGCATGACCACCCAAGTTTAATGCAAAAGTAAAGTTTCTTATTCATGAAGAAACATGATGGTGGAAGCTCATGAAATAATTTGGATGTAATTAGCATTAAGGTTTAGGAGATGAAAAAATGTGATAGTTAACTTTGGGCAATTGGTTGAACAAAAAAGTACAACTAGTTGACAAAGNTTCAACTTTTGCAAAAATGCTATTTTTTGGTATTTTATTTGTAATTGACAATAGTAAAATGTTAGTATGAATGAATGAA
TARGET=1068,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04909 Ps_contig_mira-and-tgicl-ass_franssen_35210
SEQUENCE=ATTTTAAACTAAAATCATCATGGAATTAATATGATAAATTTCCCAAACTGGTTTTATACCATAGTTGGGATGAAATATACAAGGCAATTAAAATCAGCATCACCTTCAATTACTTAACTTAATGAAACAAAAAGCAACTATATATTATATATTAGGCCTAGCTAGCGGGTGCCATATATGCCACCACAGCATTACTAACTCCTCCTAATCTACCTCTTTTTCCCCTCACACTTTCCATCTAAAACAAAGGAAACAATGCAATGCAATATATTACACACACACAGTTTTTGTGACCTTCAAAATGAAACTTATGTTTCTAGTATTGTGCCCCTGTAAATGTCTGTCCAAATTGCCAATTACTAGGCACAACGTTGTTGCTAGTGACAACCCTGCCATCACTTGTAGTAACTTGGAAAGAAAGGCTTTGACCGTTGAGGTAGTTGTTGCTTTGCCAATTTTGTCCCCAGTTTCTTGACATAGTTTGCCACCCGGTTTTTGATCCTTTGATGGATACAGAATGAACATCTCCAGCTCCACCCACGTTTGTTACCAAAACTAAGTTGAAGTATGAGTGTCCATTGATTGTGAACCTTATTCCTCCTTTCTTCACACAGGGGACTCTTCTGAAGGAAATAGGAACAATTCCAGCTTTGTATTGAGCAATTTGTAAGAAAGCAGGCTCAGCAAGATCAAAGTGCTGAAGAGGAGGGTTACACCATCCACCATTGGAATTAGACTCTGCAAAGTTTGGTGGACAAAAGTTTGTGGCAGTGACCAATATGCTTCCCGGAAGACACCATTTAGGGTCATTGTCACATCTCATCTCATAGCAAGACCCACAACTCAATCCATTGTTGAATAGAGCAGTGCTTAGTGCAGCAGTGTTGGTTCCATAGCCTTGACTATACAAATTTCCATAACCACAAGCCCCTCCCATTGTGCCTGAAGCATCTCCACCACCATAAAATGTGGCATGGGCACTTTCCCATCCACCTCCATAATCAGCAAAAGTGCCTTTAAGGCATAAGCTAAGAAGAAAGAAAGGAATAAAAATGAATGAGGTTGCTAGAAAAGCCATTGTGAGTACACTAGTACTTGTTAGTGGTTTTGAGATAGGAGAAGTAGCTTTTAATATTTGGTGGTGCTTCCCCCGTA
TARGET=273,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1643
PRIMER_LEFT_PENALTY=0.102490
PRIMER_RIGHT_PENALTY=0.061818
PRIMER_LEFT_SEQUENCE=TCTACCTCTTTTTCCCCTCACA
PRIMER_RIGHT_SEQUENCE=ATTGGCAAAGCAACAACTACCT
PRIMER_LEFT=208,22
PRIMER_RIGHT=455,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=60.062
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.1693
PRIMER_LEFT_1_PENALTY=0.102490
PRIMER_RIGHT_1_PENALTY=0.066849
PRIMER_LEFT_1_SEQUENCE=TCTACCTCTTTTTCCCCTCACA
PRIMER_RIGHT_1_SEQUENCE=CAACAACTACCTCAACGGTCAA
PRIMER_LEFT_1=208,22
PRIMER_RIGHT_1=445,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.067
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.1980
PRIMER_LEFT_2_PENALTY=0.102490
PRIMER_RIGHT_2_PENALTY=0.095496
PRIMER_LEFT_2_SEQUENCE=TCTACCTCTTTTTCCCCTCACA
PRIMER_RIGHT_2_SEQUENCE=AAGCAACAACTACCTCAACGGT
PRIMER_LEFT_2=208,22
PRIMER_RIGHT_2=448,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=0.2585
PRIMER_LEFT_3_PENALTY=0.102490
PRIMER_RIGHT_3_PENALTY=0.155993
PRIMER_LEFT_3_SEQUENCE=TCTACCTCTTTTTCCCCTCACA
PRIMER_RIGHT_3_SEQUENCE=CAAAGCAACAACTACCTCAACG
PRIMER_LEFT_3=208,22
PRIMER_RIGHT_3=450,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=59.844
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.3629
PRIMER_LEFT_4_PENALTY=0.301100
PRIMER_RIGHT_4_PENALTY=0.061818
PRIMER_LEFT_4_SEQUENCE=TGCCACCACAGCATTACTAACT
PRIMER_RIGHT_4_SEQUENCE=ATTGGCAAAGCAACAACTACCT
PRIMER_LEFT_4=178,22
PRIMER_RIGHT_4=455,22
PRIMER_LEFT_4_TM=59.699
PRIMER_RIGHT_4_TM=60.062
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_04910 Ps_contig_mira-and-tgicl-ass_franssen_35215
SEQUENCE=ACTTGCAGCAATGAAAGTTCTCCACTAATGTTGATAAACATACAACTTACAAGTTGCAACAAAGGCTTTATGATCAGTGAGTGAGTGAGTAGGTGAGAAAGGGAGAGAACAGAAAGCTTTGAGAACCCATTTAACACATTTTAATGAGGCTTCAGTGACATTACAAACAAAATAAACAAGTTGAAATTAGAATGTTACTGGTTATCAAGAAACCACTGCCTAGCCAAGGATAGCATGTTGAATGGATCAATCTATACACACCCCTTAGTTTAAGTATCATCCTTCTTCTTACATTTGTTCTGCCCAAGGCTAAGTAGGAAATTGATAAATATGTGCTCATACTCTGGCATTTTTCCATACCCAGGAAAGTAATTGATATCAATGACATAAAACACGTCCTTTGTCCCGTGTTCTCGTATCATATCAATGTTGAACAAGCGGAGTCCCAGTCTGCGACGTAGGTCCCTTGCAAGCCTCTCCAATAAGGGTCTTGGTGGATGTTCTGCAATATTTGGATCCAAATCAGCATCATCTGCAGAAGCTGCTGCACATGAAACTCTTGGAAATCGGAATAAACCATCGACATCCAATAGCTCATTTTTACTAACATTGGGTAGAGAGAAACGCCTCACAACCTTTATAGCTTCCCCAACAATATAAATCTTAAAAAGAAGACCACCATGATTAACAAACTCTTGAAGAACAAGTGGAGGTTCAAGCTTTGAAAGAGACAATTCATCATAAGCAAGACACAATTCGTGTGACTTTGCACTCCCATCCACAACTAATGGTTTAGCAACTAAAGGTAACTTCATTCCAGCTTTGGTGACTTCATAGGGAATAGTAGATGGGTTTTTAGTGATAACCAACTGGTGCGGAACCCCAACCTTGCCATGGCAGTCAGATAAATTTAGCTCTGCCACATTTTGTAGCATGGATTGTCGATTGAGCAAATGTTGAATTGCATATGGAGGGTCAAGGATGGTTACTTCGGGATGTTGATGCCTGTAATCCTCAATAATCTCACGCCACTCTTTTCCTGGCAACTTATGCAAGATAACATCAAATGGACCTTGTTCTAACAGCGGCTTCTTTAGATCAATGGCAACAAAGGAAATCCCCTTATTCCTTGCGAGGGCAATAAAGTTTGGCTTTAGAAAGCTTTTCTTCTTCTTCGACGTTAAGGCGTAACCAACCACCATTTTCTCTTCATCTTCTTCTCCGTCCGAGATTTCTCCGTTCAACTTCATGCTCACAAACCCTAAGTTTTAAAAAAAAAATCTATTCAATTTTTCTGAACTATTTCTCGACGAAATCTGAAGGTAAGAGATCGATCTTTTGTGGCTCTATATAAGCTTGGTATTACGTAATGTTGAGTTGAGTGTGAATCAAAAAAAGTGTGTGAGTAGCGTGAAATTTGATTATGAATTTCATTTTCATTTCGTTTAATTTCATTTAGGTTTTAATTTCATGGTTGTGTATTGTGTTGTGTTGCAGAGAGTAGAAAGGAATTGAGCTGACGTAAGAACGTTTTAAGTTTTTTTTTTAAAATTT
TARGET=76,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8041
PRIMER_LEFT_PENALTY=0.801836
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=GCAGCAATGAAAGTTCTCCACT
PRIMER_RIGHT_SEQUENCE=TCAAAGCTTTCTGTTCTCTCCC
PRIMER_LEFT=4,22
PRIMER_RIGHT=121,22
PRIMER_LEFT_TM=60.802
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=118
PRIMER_PAIR_PENALTY_1=0.8088
PRIMER_LEFT_1_PENALTY=0.801836
PRIMER_RIGHT_1_PENALTY=0.006951
PRIMER_LEFT_1_SEQUENCE=GCAGCAATGAAAGTTCTCCACT
PRIMER_RIGHT_1_SEQUENCE=ATTCAACATGCTATCCTTGGCT
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=242,22
PRIMER_LEFT_1_TM=60.802
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.8420
PRIMER_LEFT_2_PENALTY=0.801836
PRIMER_RIGHT_2_PENALTY=0.040146
PRIMER_LEFT_2_SEQUENCE=GCAGCAATGAAAGTTCTCCACT
PRIMER_RIGHT_2_SEQUENCE=TCCATTCAACATGCTATCCTTG
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=245,22
PRIMER_LEFT_2_TM=60.802
PRIMER_RIGHT_2_TM=59.960
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.9458
PRIMER_LEFT_3_PENALTY=0.801836
PRIMER_RIGHT_3_PENALTY=0.143960
PRIMER_LEFT_3_SEQUENCE=GCAGCAATGAAAGTTCTCCACT
PRIMER_RIGHT_3_SEQUENCE=TCTGTTCTCTCCCTTTCTCACC
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=112,22
PRIMER_LEFT_3_TM=60.802
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=1.0113
PRIMER_LEFT_4_PENALTY=0.801836
PRIMER_RIGHT_4_PENALTY=0.209460
PRIMER_LEFT_4_SEQUENCE=GCAGCAATGAAAGTTCTCCACT
PRIMER_RIGHT_4_SEQUENCE=TGATCCATTCAACATGCTATCC
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=248,22
PRIMER_LEFT_4_TM=60.802
PRIMER_RIGHT_4_TM=59.791
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_04911 Ps_contig_mira-and-tgicl-ass_franssen_35215
SEQUENCE=ACTTGCAGCAATGAAAGTTCTCCACTAATGTTGATAAACATACAACTTACAAGTTGCAACAAAGGCTTTATGATCAGTGAGTGAGTGAGTAGGTGAGAAAGGGAGAGAACAGAAAGCTTTGAGAACCCATTTAACACATTTTAATGAGGCTTCAGTGACATTACAAACAAAATAAACAAGTTGAAATTAGAATGTTACTGGTTATCAAGAAACCACTGCCTAGCCAAGGATAGCATGTTGAATGGATCAATCTATACACACCCCTTAGTTTAAGTATCATCCTTCTTCTTACATTTGTTCTGCCCAAGGCTAAGTAGGAAATTGATAAATATGTGCTCATACTCTGGCATTTTTCCATACCCAGGAAAGTAATTGATATCAATGACATAAAACACGTCCTTTGTCCCGTGTTCTCGTATCATATCAATGTTGAACAAGCGGAGTCCCAGTCTGCGACGTAGGTCCCTTGCAAGCCTCTCCAATAAGGGTCTTGGTGGATGTTCTGCAATATTTGGATCCAAATCAGCATCATCTGCAGAAGCTGCTGCACATGAAACTCTTGGAAATCGGAATAAACCATCGACATCCAATAGCTCATTTTTACTAACATTGGGTAGAGAGAAACGCCTCACAACCTTTATAGCTTCCCCAACAATATAAATCTTAAAAAGAAGACCACCATGATTAACAAACTCTTGAAGAACAAGTGGAGGTTCAAGCTTTGAAAGAGACAATTCATCATAAGCAAGACACAATTCGTGTGACTTTGCACTCCCATCCACAACTAATGGTTTAGCAACTAAAGGTAACTTCATTCCAGCTTTGGTGACTTCATAGGGAATAGTAGATGGGTTTTTAGTGATAACCAACTGGTGCGGAACCCCAACCTTGCCATGGCAGTCAGATAAATTTAGCTCTGCCACATTTTGTAGCATGGATTGTCGATTGAGCAAATGTTGAATTGCATATGGAGGGTCAAGGATGGTTACTTCGGGATGTTGATGCCTGTAATCCTCAATAATCTCACGCCACTCTTTTCCTGGCAACTTATGCAAGATAACATCAAATGGACCTTGTTCTAACAGCGGCTTCTTTAGATCAATGGCAACAAAGGAAATCCCCTTATTCCTTGCGAGGGCAATAAAGTTTGGCTTTAGAAAGCTTTTCTTCTTCTTCGACGTTAAGGCGTAACCAACCACCATTTTCTCTTCATCTTCTTCTCCGTCCGAGATTTCTCCGTTCAACTTCATGCTCACAAACCCTAAGTTTTAAAAAAAAAATCTATTCAATTTTTCTGAACTATTTCTCGACGAAATCTGAAGGTAAGAGATCGATCTTTTGTGGCTCTATATAAGCTTGGTATTACGTAATGTTGAGTTGAGTGTGAATCAAAAAAAGTGTGTGAGTAGCGTGAAATTTGATTATGAATTTCATTTTCATTTCGTTTAATTTCATTTAGGTTTTAATTTCATGGTTGTGTATTGTGTTGTGTTGCAGAGAGTAGAAAGGAATTGAGCTGACGTAAGAACGTTTTAAGTTTTTTTTTTAAAATTT
TARGET=1165,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2294
PRIMER_LEFT_PENALTY=0.075346
PRIMER_RIGHT_PENALTY=0.154023
PRIMER_LEFT_SEQUENCE=CTTGTTCTAACAGCGGCTTCTT
PRIMER_RIGHT_SEQUENCE=GGTTTGTGAGCATGAAGTTGAA
PRIMER_LEFT=1072,22
PRIMER_RIGHT=1260,22
PRIMER_LEFT_TM=60.075
PRIMER_RIGHT_TM=60.154
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.2963
PRIMER_LEFT_1_PENALTY=0.075346
PRIMER_RIGHT_1_PENALTY=0.220992
PRIMER_LEFT_1_SEQUENCE=CTTGTTCTAACAGCGGCTTCTT
PRIMER_RIGHT_1_SEQUENCE=ATGAAGAGAAAATGGTGGTTGG
PRIMER_LEFT_1=1072,22
PRIMER_RIGHT_1=1212,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=60.221
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.3692
PRIMER_LEFT_2_PENALTY=0.215225
PRIMER_RIGHT_2_PENALTY=0.154023
PRIMER_LEFT_2_SEQUENCE=GGATGTTGATGCCTGTAATCCT
PRIMER_RIGHT_2_SEQUENCE=GGTTTGTGAGCATGAAGTTGAA
PRIMER_LEFT_2=993,22
PRIMER_RIGHT_2=1260,22
PRIMER_LEFT_2_TM=60.215
PRIMER_RIGHT_2_TM=60.154
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.4362
PRIMER_LEFT_3_PENALTY=0.215225
PRIMER_RIGHT_3_PENALTY=0.220992
PRIMER_LEFT_3_SEQUENCE=GGATGTTGATGCCTGTAATCCT
PRIMER_RIGHT_3_SEQUENCE=ATGAAGAGAAAATGGTGGTTGG
PRIMER_LEFT_3=993,22
PRIMER_RIGHT_3=1212,22
PRIMER_LEFT_3_TM=60.215
PRIMER_RIGHT_3_TM=60.221
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=0.4617
PRIMER_LEFT_4_PENALTY=0.307639
PRIMER_RIGHT_4_PENALTY=0.154023
PRIMER_LEFT_4_SEQUENCE=AAGGAAATCCCCTTATTCCTTG
PRIMER_RIGHT_4_SEQUENCE=GGTTTGTGAGCATGAAGTTGAA
PRIMER_LEFT_4=1110,22
PRIMER_RIGHT_4=1260,22
PRIMER_LEFT_4_TM=59.692
PRIMER_RIGHT_4_TM=60.154
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_04912 Ps_contig_mira-and-tgicl-ass_franssen_35219
SEQUENCE=TGGAATTAGAAATGATTGTAATTTTGAACATATTTGTTTCAGGAATGAATAGGAAGTTTGAAGGTTATTGGTTAGCTTGGAAATTTCGGCTTGGATGCGGCTACGAAGCGCATTGGATGGCGGGGCTGTTTTGGAATTCTGTAAAGCTTCTTCATGTTCCACGACTTCAATAGATGAATTGATCGAGCGACAAGCAGCAGCAGCGGATTTAACTTTTAGCTGACCAATATATGCATCCGTGTGTATAATCTGTCTATGCATATTATTCAACTCAAACTTGTCATGATCAAGAACATTGTATCTATGAATCATGTGAGGTTCTAATCCATTTGTAGCGTACCGTAAAGTTTTAAAATGTCGGAGATTAGCTTTAGTGCCTCAAATTTGTAGTTTCCGTTTGTTTATGGTTGTTTGTAATTAGGTGCCTAGTATAATCCTTAAATTTTTGTAGTTGTATAGCTTTATAA
TARGET=193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7009
PRIMER_LEFT_PENALTY=0.598109
PRIMER_RIGHT_PENALTY=0.102789
PRIMER_LEFT_SEQUENCE=TAAAGCTTCTTCATGTTCCACG
PRIMER_RIGHT_SEQUENCE=ACACGGATGCATATATTGGTCA
PRIMER_LEFT=141,22
PRIMER_RIGHT=241,22
PRIMER_LEFT_TM=59.402
PRIMER_RIGHT_TM=60.103
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=0.7562
PRIMER_LEFT_1_PENALTY=0.309858
PRIMER_RIGHT_1_PENALTY=0.446308
PRIMER_LEFT_1_SEQUENCE=AGCTTCTTCATGTTCCACGACT
PRIMER_RIGHT_1_SEQUENCE=TTTACGGTACGCTACAAATGGA
PRIMER_LEFT_1=144,22
PRIMER_RIGHT_1=345,22
PRIMER_LEFT_1_TM=60.310
PRIMER_RIGHT_1_TM=59.554
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.7657
PRIMER_LEFT_2_PENALTY=0.309858
PRIMER_RIGHT_2_PENALTY=0.455824
PRIMER_LEFT_2_SEQUENCE=AGCTTCTTCATGTTCCACGACT
PRIMER_RIGHT_2_SEQUENCE=GAGGCACTAAAGCTAATCTCCG
PRIMER_LEFT_2=144,22
PRIMER_RIGHT_2=379,22
PRIMER_LEFT_2_TM=60.310
PRIMER_RIGHT_2_TM=59.544
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.7657
PRIMER_LEFT_3_PENALTY=0.309858
PRIMER_RIGHT_3_PENALTY=0.455824
PRIMER_LEFT_3_SEQUENCE=AGCTTCTTCATGTTCCACGACT
PRIMER_RIGHT_3_SEQUENCE=AGGCACTAAAGCTAATCTCCGA
PRIMER_LEFT_3=144,22
PRIMER_RIGHT_3=378,22
PRIMER_LEFT_3_TM=60.310
PRIMER_RIGHT_3_TM=59.544
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.8901
PRIMER_LEFT_4_PENALTY=0.309858
PRIMER_RIGHT_4_PENALTY=0.580198
PRIMER_LEFT_4_SEQUENCE=AGCTTCTTCATGTTCCACGACT
PRIMER_RIGHT_4_SEQUENCE=GGCACTAAAGCTAATCTCCGAC
PRIMER_LEFT_4=144,22
PRIMER_RIGHT_4=377,22
PRIMER_LEFT_4_TM=60.310
PRIMER_RIGHT_4_TM=59.420
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_04913 Ps_contig_mira-and-tgicl-ass_franssen_35223
SEQUENCE=AGACAGTACATGATAACTTTTTAAGAAGTAAAAGTATGCTTATATTGTATACTACTTGCATTGCCTAAAAGCTTTAGATTAGTACATTATTGTCCTCTTACATACATAATAAGAACCAACGGATGAAAGATTTGTTCACTATTTAACAAAAACTGGTTTANTTGATAGGGATTCCCTGCCATGAGGCCTGTATAGAGTTTCATGCTCTGAAGTTGAAGGCATTACTGGTCTAATGCCCCCTGGATTGAGAGGAAAAAGGATTTTGTAGTAACCATCCATAATTTCTTGAAAATTACAAGTGTCTGCAACTTTGGGAATCTGATAAATGTCACGCATGTAGGCATGTAAGTTAGAATACTCATACAACTTCTTCTTGGTGCATTTAAACAGAACATTATAAGCAATGTCAAACCGAATTAAAGTAGTAAAAAGACATATATCAACAAGTGTGAGCGTGTCTCCGCACAAGTAACGAGAATTTGACAAGTGATCCTCCAACTTGTCTAGTGTAAAAAACAACTCATTTACCGCTCTATCATATGCTTCTTGGCTCTGAGCAAATCCGCACCTATAAACACCATTATTCACATTTGGATATATCACTTGATACCATTCTTCAATCTTATCCTTTAATTCAGGGGGTGAAAGATCCAACTCCGGGTTACGGGCCAACCCATTTAACCCGGAGTTAAACAATTGAATTACATCGTAACTTTCGTTACAGATAACATCTTTGGATTCTTTACTCCACAACATTGGCACTGTGGATCTTCCATCATACCCACCTCTTCGAAGCCTATAAACTTCCTTCAAAGTCTTGCAACCATTCGCTTTATCCAAACTAGGACCAATTACCTGACCCGTATCTACACCACCGACCCGTTTAAATTCCCACGACCCGTCCATGCCAGGAGACGCAACTGACACCGGCACGGCATCTTCTAAGCCTTTGAGTGCGCGTACTATGAGAGTACGGTGGGCCCACGGGCATGGCAGGCCAACGTAGAGGTGGAGACCATTGGGTGGAGGACCGGAGAATCGGAATTTAGAAGCGGGTCTTGAATACCCGCCGGTGGGGTCAGAGGGAGCCAGCTGTGACATCATGAGCTGCCACGTAGAATTCCAGGCGGTGCGCACGGTGGTGACGAGAAGCCCTGGCGGGAGGGACCGACCCCAGAGAAGATTGGTCACTGCACTCAGTAGAGAAGAGGAATTACTGTTAGAATTGTTTTTGTTGTTGTTGTCAAGTGACATCCTCAGGCGAGGACGGATGGCGTAGCTCCCGGCGAGCCTCCGATTATGGGAAGTTGTTGCCGGAGAAATGACGACGAATTGATGGGAGGAAGGGATAGAAGCACACAACAACGACATTCTTTAGAATTTAACCTGCCACCTTAACCTGCCCCC
TARGET=1232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0903
PRIMER_LEFT_PENALTY=0.085951
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=CAGAGAAGATTGGTCACTGCAC
PRIMER_RIGHT_SEQUENCE=GTTGTGTGCTTCTATCCCTTCC
PRIMER_LEFT=1174,22
PRIMER_RIGHT=1362,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.0903
PRIMER_LEFT_1_PENALTY=0.085951
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=GAAGATTGGTCACTGCACTCAG
PRIMER_RIGHT_1_SEQUENCE=GTTGTGTGCTTCTATCCCTTCC
PRIMER_LEFT_1=1178,22
PRIMER_RIGHT_1=1362,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.2163
PRIMER_LEFT_2_PENALTY=0.085951
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=CAGAGAAGATTGGTCACTGCAC
PRIMER_RIGHT_2_SEQUENCE=TTGTGTGCTTCTATCCCTTCCT
PRIMER_LEFT_2=1174,22
PRIMER_RIGHT_2=1361,22
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.2163
PRIMER_LEFT_3_PENALTY=0.085951
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=GAAGATTGGTCACTGCACTCAG
PRIMER_RIGHT_3_SEQUENCE=TTGTGTGCTTCTATCCCTTCCT
PRIMER_LEFT_3=1178,22
PRIMER_RIGHT_3=1361,22
PRIMER_LEFT_3_TM=59.914
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.2616
PRIMER_LEFT_4_PENALTY=0.085951
PRIMER_RIGHT_4_PENALTY=0.175676
PRIMER_LEFT_4_SEQUENCE=GAAGATTGGTCACTGCACTCAG
PRIMER_RIGHT_4_SEQUENCE=TCGTTGTTGTGTGCTTCTATCC
PRIMER_LEFT_4=1178,22
PRIMER_RIGHT_4=1367,22
PRIMER_LEFT_4_TM=59.914
PRIMER_RIGHT_4_TM=60.176
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_04914 Ps_contig_mira-and-tgicl-ass_franssen_35233
SEQUENCE=TGCTACTAATTCAGCTTCTTCCGGTGGTGTTGGTACTGGTRCTGGTGTTGCTGGTGCCGGTGGTGGCGAGCATAACAGAATTCTCCAGATGGGAGGTGGAAGTGTAAACTACGGTTTTGGCGGCGGGAATAACGAACAGAGTAATTTGCAGGATTTGATGAACTCTTTTGCTGCTACCGGGAATGCTTCAATATTTGAAACGGGAAGAGGAGGTGTAGGGCTTGGAAGTGGAGGTGCAGATAGCAGTCAACTAACTCGTGACTTTTTAGGAGTTGGTGAGATAGTGAGAAACATGAGCGGTGTTGTTGGAGGACAAAGGGAACAACAACAACAACAACAGAATCATCAACATGCAGCAGCTGCATTTAACCTGCCAGTTTCCATGGAAGGAGATCAAAGAAACATTCATGCTGCACAGTCCTTTGGAGGCGGAGGAAGAGGAGGAGCTGGTGGTGGAGCAGGAGCAGGAAATTTTCACTAATCATTATCATCTTTCATAAAGGATTTCAACATGCTCCATTGGAACTTGATCTAATTAAAGATTTTGAGACTACTGTGAAGAATATGTGGAAATTCCTGAATTTTCTTGAAGCTTCTTCAAACTCTGTTAAATCCCCCTCCCCCTAATTTTTTTTTTCCTGATACATATTAGCTTCTAAGTTTTTTTTATGGTTTAATTTAATTAGTATTTTTGTTATTGGGTAATTGATTATTTATCATATATATTTAAATAGAACG
TARGET=321,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04915 Ps_contig_mira-and-tgicl-ass_franssen_35235
SEQUENCE=GTCGGGGTAAGTTGGAAAAGAAAATCATTTAAGAAGTCTCTTTCTTGACCTCTCTCACANGCCACACACTCTCACACAACACAACACAACACAAAAGCCTGTTCCATTTCCAATCTCCATATTTGGTTTTGGTCAAGTAAAACCAAACCAAAACCTCTTACCCTATATACCTGCTTTGATTCTGTGAATCTGCAAAAACAGTATTCTCATGCCTACATACACCTCATAACCATATATAATCACCAATCTAACAAGATCTGATTACGTGTATTGCATGTCTCTTTCTTGAGCTGAGTGAACAACACATCATAACTCATCAAGAGACACAGTTACAGATGAAGAGAGAGAAAGTGTGTTTTTCTCTGTGTGTCCAAAAAA
TARGET=74,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6152
PRIMER_LEFT_PENALTY=0.220453
PRIMER_RIGHT_PENALTY=0.394711
PRIMER_LEFT_SEQUENCE=TTTCTTGACCTCTCTCACANGC
PRIMER_RIGHT_SEQUENCE=GCAGATTCACAGAATCAAAGCA
PRIMER_LEFT=40,22
PRIMER_RIGHT=192,22
PRIMER_LEFT_TM=60.220
PRIMER_RIGHT_TM=60.395
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.1250
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.6152
PRIMER_LEFT_1_PENALTY=0.220453
PRIMER_RIGHT_1_PENALTY=0.394711
PRIMER_LEFT_1_SEQUENCE=TTTCTTGACCTCTCTCACANGC
PRIMER_RIGHT_1_SEQUENCE=TGCAGATTCACAGAATCAAAGC
PRIMER_LEFT_1=40,22
PRIMER_RIGHT_1=193,22
PRIMER_LEFT_1_TM=60.220
PRIMER_RIGHT_1_TM=60.395
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1250
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=154
PRIMER_PAIR_PENALTY_2=0.6969
PRIMER_LEFT_2_PENALTY=0.220453
PRIMER_RIGHT_2_PENALTY=0.476404
PRIMER_LEFT_2_SEQUENCE=TTTCTTGACCTCTCTCACANGC
PRIMER_RIGHT_2_SEQUENCE=GGGTAAGAGGTTTTGGTTTGGT
PRIMER_LEFT_2=40,22
PRIMER_RIGHT_2=162,22
PRIMER_LEFT_2_TM=60.220
PRIMER_RIGHT_2_TM=60.476
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1250
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.8116
PRIMER_LEFT_3_PENALTY=0.220453
PRIMER_RIGHT_3_PENALTY=0.591111
PRIMER_LEFT_3_SEQUENCE=TTTCTTGACCTCTCTCACANGC
PRIMER_RIGHT_3_SEQUENCE=AGGGTAAGAGGTTTTGGTTTGG
PRIMER_LEFT_3=40,22
PRIMER_RIGHT_3=163,22
PRIMER_LEFT_3_TM=60.220
PRIMER_RIGHT_3_TM=60.591
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1250
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=1.0861
PRIMER_LEFT_4_PENALTY=0.220453
PRIMER_RIGHT_4_PENALTY=0.865646
PRIMER_LEFT_4_SEQUENCE=TTTCTTGACCTCTCTCACANGC
PRIMER_RIGHT_4_SEQUENCE=TGTGTTGTTCACTCAGCTCAAG
PRIMER_LEFT_4=40,22
PRIMER_RIGHT_4=305,22
PRIMER_LEFT_4_TM=60.220
PRIMER_RIGHT_4_TM=59.134
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.1250
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_04916 Ps_contig_mira-and-tgicl-ass_franssen_35237
SEQUENCE=TACTCCTAGCCCACTTCTAAACAAAAAANAAGTTTCTTGGAGCAGCTGNTAAGTTCACGCCTGGCAAGTCTCGTTCGCCTCCAGCGCTGCAGAATCTGTTTAATTGGATCTAAACTTAAATAGCATAAACTGGGGAGAAAGTGGGAATACTCCTTCTTCTAGAAGTTCCAATTCAATAGACAGCCACTTGACCCGAGATATAGGTCTCCAGTTTAACGTAGTCGAGTTTAAAAAGTCTACTGAAGAGGTGAAGTTGCGCGCGGCCACTGATGAAAAGGTTAAAAAAACTGCGACAAAGAAGCGGAAGTCAGATGTTCATGAACCTCCTTGTGAACACGAAGATGAGGTAGAAATGCGCAATAGGTTTTATGAAAGTGCATCTGCAAGGAAAAGAACTGCGACAGCTGAAGAAAGAGAAAAGGCTATCAATGCAGCAAAAGCATTTGAACCATCTAATCCTTTCTGTCGAGTCGTCCTGCGACCCTCCTATTTATATAGGGGATGCATAATGGTGATCTGTTGAATCCAACGCTAGTTTTTTGTTTTGTTTTGTTTTTGTTTTCTTTGACAATTTGATCTTGCATACTAATTTTATTTTGTTTCC
TARGET=538,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04917 Ps_contig_mira-and-tgicl-ass_franssen_35246
SEQUENCE=TCCTCGATTTAATCTCTACCTTACTCCAATTGGAAAAAACAAAAAGACAATAAATAAACCAAYCAACATGAGATTTACAGTGATATACGTATGGGTTCGACGACCTTGTGGTGATTGGAAGGACTGTGAGTTCGTCAGAAATGAACTTGAAATTATAATGGAAACGGAGTGCGGCTAGACCCATCCTTCTACATTATTTCCTTTGGTGCTATGCTCCTCTTATTGTTGTAGAGCGGCTGGTTGGTTGCGTTTGAATCCGACATCGGAAGGAGAAGACGTCCGTGAATGTGAAGGAGAAGACGCGTGGTGGCTTTTGCGGTGGCCACGAAGGAAGTGTGGGAGGGGAACGGAGGCGGTTGTGAGGGTTTATTGTGGATGGCAGTAAGATGTCGAAGGTGGCGGCGGTGATTTCGTGAGGAAGGAGATTCATTGAGTTTCGTTTCGTAAAAAACGGTTTCACGGTGTTGTTGGTTGGTTGCGGTTGTGAGTTGAGTGAAGACGGAGAGTGGGATTTGGACGGTGGAGATGGCGGTGTGTGGTTGTGACGAAGACGTAAGGGTTTGGACGGTTGTTAGAAAAAGAGGATCGCGGTTGTTTCTTGTTTTTCCGCCAGCGGAGTAGTTTGAAGGAGGGTAGGG
TARGET=467,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04918 Ps_contig_mira-and-tgicl-ass_franssen_35247
SEQUENCE=TTTGGGGCCGATGTTGTTCCACCCTCGCTGAATCGGGGGAAGACGGGTATGCTGCTGTGGGTGCTGCATTTTAAGACGCTTGGCATTTTCTTCATTTTGCCGTTGTTGCTGACGCTTTTTCTTGGTTTGAAACTCATGTGAGGACTCGTATTTGGGTAAACTCTTGGGATCACATGGTAAAGGATCAGTCCAGAAGTACTCAGCATCAAGTGCATCCTTCGCAGGAATTCTCTGAGCTGGATCAAGTGTGAGCATCTTCTCCAACAACTCCAATGCATGACGATCAAAGTGTCTGAAAACATCCCTCAGACGCCTTTTCATCGGTCTTGTCGGCTTAAACTGATTGTACCAAGGAGTCTTTTGAACTCCTGGCCAGTTCACCTCATCAGGTGCTCCACAAAGCTCAAAAATCTTATTTAGTTGCTCTGGCTCATCTTTTCCAGGAAAGATGGGCTTCCCATGAAGCAGCTCGGCGAAAATGCACCCAACGGACCACATGTCTACAGCTGGCCCATACCTTGTAGTCCCCAATAGTAACTCGGGGGGTCTGTACCATAAAGTAATGACTCTATTTGTAAGGTTTGCATTATGCTCATTCGAAAATGACCGTGCCAATCCAAAATCTGCCAGTTTCAGATTACCTTCATTATCTATGAGAAGATTTGATCCTTTGATATCACGGTGAAGCACTTGATTGACATGACAATAATGAAGTCCTGTCAAGAGCTGCCTCATGTAACACTTGATCTGAGGGACAGTAAACCTCATGCCAGGTCGGTCTGCAAGACCTGTTAAATCGTGGTCCATATATTCGAAGACCATATATATGCCACCTTTGTATTTGTTGCCATCTGGCCTTCCTTGATCATCTTTCTCAGGACCTGGAGATGTGACGATTTCCTTCAATTTGATTACATTTTCATGATGTAGTTTCTTTAGAATTTTGATTTCCCGTATAGCAGTTATTGGAAACCCTTCTCTCTCATTGTCCATTCGTATTTTCTTCAAAGCAACAATTTCACCAGTTTCAATTTCTCGAGCCATGTAAACCTGACCATAAGTTCCTTCACCAATTTGCTCCAATTTTTCAAAGCAATCAACGCTTCGGGATCCCCGCGAAGGCGATTCAATGACGTTCAGTTGCCCTGGGGCTGCCATTGCCATTACAAGCACAAACCCTAAAACTCTAGCGAAAGAAAGAAAGAGGGTTAAAATAAGAGTATGAATCCAAGTGT
TARGET=1192,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04919 Ps_contig_mira-and-tgicl-ass_franssen_35257
SEQUENCE=AATTTTGGATTGCTTAATTATAACAATGTAGTTTTTAACTAACACAAAAAAAANNATTTAGATTTATAATCCAAATTTTTGTATGAATAGNNAAAAAAAATRCTCCATCAAATCAAAGCACATATAGATAAGAGAAAATTGTTGAGCGAGAATCCCAATTTAGATCTTAGATGGAACTATCATACTTTATTTATCTCCCAGACAAAATTCGATTTGCCATGATTCCCTTTATTTGGAAACTGTAGGCTTAGCACAGGAAAAATTAGATAAGAGAAAATGAAAACCACCTCTTGATCATATTATAAACTTCCTTGGGCTTAAATGTCTGGGCCACATGTCCAGCACCCTTTACGGTCACATAGGTTAAGTAGTGGTCTTCTTTCATTTTGTAAATTTTTGTGTATCCTGCAACTTGACCTTCAACAAACCATGCACGCCATTTGTCATGTATGCTCATATTGAATGACCTTATCCATTCTTGTGTACCCATTTGTGGTACTACCATGTCAAGATCAGAACTATAAATAAGAGCTTGGATGTTGGCGTTGGTGAGATTGTGATAATAATCTACAACACTAGGTAGATTTTTTGTGTATCCCAAAGTTTTGTTGCATCTCAAGAAATCCCCTTTAGTTCCCTCTCTTATATGAAGGGCCTTCCTTACATTTTCATCGTTTGCCCATGTGTTAACAAGGATATGATAATAACTTCTACACTGTAGTTCTTGATCATCTTGAACTGGTTGTTGAATTACTTGGTTCACAGCAGGTGTAGTCATACAGACAGGTTCTAAAATATTTTGTTCATTTATATAATGAACGAGCTCATAATAAGCTTCATAATCCGAGACACACTTTGTGTTGTCTGGATCGATGTTCACATAATCACCCTTACAATTTTCTTCCAACGACTCATAAAGTTCTTTTGGTATTAGAGACATATGGTAAGCATATAAAACTCGTGTGTTATAATACTGGTAGGTGTCAACAGCTGGGCTTGCCAACACATAACCTTTGATGTTTATGTGTGGCATATGTCTTTCTTTGTATCCTTCATATACTTTTTGAACAAGAGGACCAGTAGTTATTCCTGAGTATGAGCCACCGCCAATATAAAATGGATTTGAGCTAAAATTTGAATGATCAATAAACCACTTTTGTAAAAAAGAATACGCATGATCCACCCAGAGTGTATCACTGCTATAATAGCCTTCTTGCGTTTCTGAGTAAGAAAATCCAGTCCCAACTGGCATATCTATGTAAATCATATTCAACATATGTGTCCAACCATAAGGATTCAATTTAAGCTTTGGAAGGTTCTCATTGTAATCACCATCCATTACTAAAGGCCCATTTTCAAAAAAGAATGCCGAAAGGCCAGAGCATCCTGGACCTCCAACCAACCAAAGCAAAATTGGATCAATTAAGGGATTTCTTTGAGACTCTACAAAATAGTAAAATATTTTCACCTTTTGTCCTTCTCCCACTCCTATGTATCCAGTTTGAAGGTTGAATGGAAGTTGGCCATCAAAACCCGGCAGGTTGTTGACAATAGAACCGGAAGAAACAATGAATGGCAGAAGCATCATCAGAGTAACTATGATAACACAGCTACATCTCCAACCCATCATCACACAGAAAAAAGAAGGGTTCTGAAATGATCAAGGTAGGTGGAAGGAAGGTCAACTGAGAGCTTCACATTTGGAATTAGGATATAGGGTGCTCATTGCATTATTGCTTACTATTTTNAAAAATGTGTTGGAT
TARGET=1395,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04920 Ps_contig_mira-and-tgicl-ass_franssen_35258
SEQUENCE=TCGGGGGAACCTTAGTTTTGTTTCCCATCTTCAACTTGTTTGGTGATAATGGCAATATCTTCCTTCCTCTTCAAACTGATATTGCTGTTTCTCACCATTCATGATTTATTTATTATGCAAAGTCATGGCCTAAATTTTGGAATAAACTATGGACAAATAGCAAACAATTTACCCTCTCATTCACGTGTAGCAGCACTTATAAAATCATTGAATGTCACAAGAATAAAACTCTATGATGCTGATCCAAATGTTCTCTCAACATTCTCAAATTCAAATGTTGAATTCATGATAGGACTTGGAAATGAGAATCTTCAGAATATGAGAGACCCTATTAAAGCACAAACATGGATTCAACAAAACGTTCAACCTTACCTTTCAAAAACCAAAATCACTGCAATTAACGTAGGAAACGAGGTACTAGGTAACAATGATATTAACAGTTATATAAACCTTCTTCCAGCAATGCAATCAGTGTATAATGCACTTGTTAGTCTTGGATTATCTCAACAAGTTACCGTCACAACTTCGCATTCTTACGTCATTTTGTCGAATTCATTTCCACCTTCATCTGGTTCTTTTAAAGAAGAGTTCATACAATATATTCAACCACTTCTTAGTTTCCATGCTCAAACGAAATCGCCTTTTCTGATAAATGCTTATCCTTTTTTCGCATACAAAGGTGATCCAGAACATGTTTCATTAAACTATGTCTTGTTCCAACCAAATTCTGGTTCTGTTGATCCTGCTACAAATCTTCATTATGATAACATGTTATATGCTCAAATTGATGCGGTTTACGCCGCTATAAAGGCGTTAGGACACAACGATATCGAGGTTAAGATTTCCGAGACGGGTTGGCCTTCTAAAGGTGATGCTGATGAGGTTGGTGCTTCACTACAGAATGCTGAAATTTATCATACTAATTTGTTGAAGAGGATTGAGATGAGACAAGGAACCCCTGCAAAACCTTCTGTTCCTGTTGATATTTATGTTTTTGCACTTTTTAATGAAGATTTGAAACCTGGTCCTTCTTCTGAGAAAAACTATGGCCTTTATTATCCGGATGGTACTCCGGTTTATAACATCGGTTTGCAACAAAGTCAAGGCGGTTATTTTCCAGAAATGGTTATCGAATCCAAATCCAATATTTTGTCCAACAATTTTGTCGTCAGCATACTTGCATTATTCTTGGTGTTTGTTTGGGAGATTAGAAGATTTTGACGAGGAAAAAATGAAAACCAAGAAATTTCAAATGGACTATTCTTTTATGGAAGGACTTGTTGAAGGGAGATAAAAGATAAAAGCAGAAGAGTTTTTTTATTTGGAAAGCAAGTTCTACATTTTTTGTGCACAGCCATTGGAGGAGCTTTTGGATTCTTAGTTTGTACTATTACTTCAAAAAAACATTCATGTAACATTGTTGATTTCACAGTTTTAGCATTGATTAAAGGTAGAGTTTAAATTTTTTATTTATTTATAGAGAAACTAGATGTGGAATATGGAGTGTTGTATTTCTAGGTAGAACCTAGAAATTAGATGTGTAATATGGAGTTTTGTATTTATTTTTTGACAGAAAGTTTATATATAGAAAATAGAATTTCATTTTTTAATTAGAACNGAAAGAAAATAAAGATAATGTTGTATATAAGT
TARGET=1466,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.6609
PRIMER_LEFT_PENALTY=0.196811
PRIMER_RIGHT_PENALTY=4.464090
PRIMER_LEFT_SEQUENCE=TGGAGGAGCTTTTGGATTCTTA
PRIMER_RIGHT_SEQUENCE=CAACACTCCATATTCCACATCTAGT
PRIMER_LEFT=1358,22
PRIMER_RIGHT=1508,25
PRIMER_LEFT_TM=60.197
PRIMER_RIGHT_TM=58.536
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=4.8086
PRIMER_LEFT_1_PENALTY=0.344527
PRIMER_RIGHT_1_PENALTY=4.464090
PRIMER_LEFT_1_SEQUENCE=TATGGAAGGACTTGTTGAAGGG
PRIMER_RIGHT_1_SEQUENCE=CAACACTCCATATTCCACATCTAGT
PRIMER_LEFT_1=1266,22
PRIMER_RIGHT_1=1508,25
PRIMER_LEFT_1_TM=60.345
PRIMER_RIGHT_1_TM=58.536
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=4.8992
PRIMER_LEFT_2_PENALTY=0.435099
PRIMER_RIGHT_2_PENALTY=4.464090
PRIMER_LEFT_2_SEQUENCE=ATTGGAGGAGCTTTTGGATTCT
PRIMER_RIGHT_2_SEQUENCE=CAACACTCCATATTCCACATCTAGT
PRIMER_LEFT_2=1356,22
PRIMER_RIGHT_2=1508,25
PRIMER_LEFT_2_TM=60.435
PRIMER_RIGHT_2_TM=58.536
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=153
PRIMER_PAIR_PENALTY_3=5.0206
PRIMER_LEFT_3_PENALTY=0.196811
PRIMER_RIGHT_3_PENALTY=4.823778
PRIMER_LEFT_3_SEQUENCE=TGGAGGAGCTTTTGGATTCTTA
PRIMER_RIGHT_3_SEQUENCE=CACTCCATATTCCACATCTAGTTTC
PRIMER_LEFT_3=1358,22
PRIMER_RIGHT_3=1505,25
PRIMER_LEFT_3_TM=60.197
PRIMER_RIGHT_3_TM=58.176
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=5.1683
PRIMER_LEFT_4_PENALTY=0.344527
PRIMER_RIGHT_4_PENALTY=4.823778
PRIMER_LEFT_4_SEQUENCE=TATGGAAGGACTTGTTGAAGGG
PRIMER_RIGHT_4_SEQUENCE=CACTCCATATTCCACATCTAGTTTC
PRIMER_LEFT_4=1266,22
PRIMER_RIGHT_4=1505,25
PRIMER_LEFT_4_TM=60.345
PRIMER_RIGHT_4_TM=58.176
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_04921 Ps_contig_mira-and-tgicl-ass_franssen_35262
SEQUENCE=GGGGAAAAAAACGGTTTTTTAAACGGGGTTTTTTTTTTAAGGGGGGTTTTTACCCGGTTAAAAAACCCCCCGTAAAAAACCGTTTTAACCCCGTAAAAAACCCTTTTTTAAAAAGTTTTAAGGGTTCCGAACGGTTAGGGTTCCCGTAAAACCCTTAACGGTCGTTCCCTTCCACTAATTTGCCCATTTCAAAGTTCATCATGTTCGTAATCGTCCATGTACTCGTGGGGATCCGGCCTTCTACGACGCCTCTTATCTCCGTATCTACTGCCCCTCTCTCTCCTCCTCTTTTCGCCCTCTTCTCTTGCCCTTTGTGCCTCCTCTTCTTCCCGAGCCCTTCTTTCTTTTTCCGCTTCTTCAAAAGCTTCTTTTTCAGTCTCTCTATTATTAATCATAAATTCTTCCACAGCTTGCTTTGCATATTGTGGATCGTCACAAATTAAGATGTTGTCATAAATCGAACCGGCCTTCACCTGCCACACTTCAATGCCCACATACTTTATTGGTTTCAGCACATAAAGATCAGGATCGTCCTCAAATTCTGGGTTATCAATCCATGGAGTCCTCCACTTTCCTTTGTAGTTGGGGTTCTTGATTTTCTTCCGTTTCCACGGTCCTTTGTATGCTGGATTAGGAATTTTTGGCGCTTTCCATAGTCCATCATCATCATCCCAGTCAACAGGCTTCTTGGCTTTTGGATCAGGGATTTCAGCTGGAATTGAATTATAGCCCTCTGGTTTGACATCATTAGGGTCCTCAATGTACTCTCTATCATCCCAGTCTGCTGGCTTTCTCGCCTTGACATCTTTGATTTTCCTTGGAGGGAGAATGTCCCAATCCGTGTACATGCTTCCAGAATCTCTTTCTCGATTATCAACAAGGACACTATAAGAGGCATCAGGCCTTAGAATCAACGTATAGAAATGAGTTAATTTGTCTGTTTCGCATTCGAGATCCTTTCTAATGGGATAATTCTGCCCCTGGTATGACAATATAACATGAAGCTTCTTTGTCTGAGTGCCGCATAGATCCGGTCCAAACATCAAACTATAAGGAGTATCACCGCCAAACTTCTTCTGGTTAACATAACCCGAAAGAAGTTTGATATAACCACCACCACATTCAACATCCTGCTCAAATTTAATAGAATACTGAAGCACCAGGGTTCTGTTCACATTAGTCAACTCTGGTATCTTTGCAGATATCGAAAAATGCTTGGCATCATTATATGTCTGAATTCCTTTGTCATCAGGATCTCCAGGCCATTTTCCAGCAGTATACTTAAAGGTACCTGCTTTTCCTTCACTCCTTTTCCAATCAGACAAAACCCAACGACTTTTCCACCCATCTTGAAATTTTTCTTCGAAAAAGACTTCGGCAAAGGATGAATGAGAAGTTAGTAGAATGAAAAGCAAGAGGTTGAAAAGATGGGTTGAGATTCCCATTGCATCTCTTCCAATTCAGACAGTGGAACATCTRATNNCNANTTTAAGAA
TARGET=659,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04922 Ps_contig_mira-and-tgicl-ass_franssen_35266
SEQUENCE=AGACCGTCATCTACTCCGCGTTCTCATTGGCACCCTCCTCCGAGTAAGAGTAATGGCTATTTGCGTGTTCGATGTAATGGTGGGCTAAATCAGCAGCGTACTGCAGTTTGTACCCTTTTTCCTCTTCTCACTGTAAAATGTGATGTCTTTTTTTAAGAGTATATGGGTTGTTATTTTCAACTCTTGATTTAGNGCATTATGTATGCTTGTGAAGCTAGGTGAGTGAATCTAGTGCTTGTTGGTTAGGAGTTGGGGTTTCTTTTAGAAATTAGTTAGTTAGTT
TARGET=269,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04923 Ps_contig_mira-and-tgicl-ass_franssen_35273
SEQUENCE=ATGTGAAGGAGCTTGTTCCTGTTGCTCTCGATAAAGCAAAAGAGGTNGTTAAGGTTAAAGGTTTTNCTTGGTAGATGGAAGATGATCATATCTAGATTAGAACAGATTCCGTTGATGTTATCGAATTTATCGAGCCATCCGTGTTTCTCGAAGAATGCTCTTTGCAAGGAGCAGTTGCAGGCTGTGTCGAATTCGCTCAGAGACGTGGTTGAATCGGCTGAGTTGTGTTTGAAGGAGAAATATGAAGGTAAGCTTCAGATGCAGAGTGATCTGGACGCGTTGATTGGGAGAATGGATTTGAATTTACGAGATTGTGGACTTTTGATCAAAACTGGTATGCTCGGCGAGGCTACTTTGTCGTCGTCGTCGTCCGTGTCTGGTTCTGTCGAACAATCAGATGCTGCAGCACATAACAACATAAGGGAGTTACTCGCACGCCTTCAGATTGGTCATTTGGAATCGAAGCACAAAGCTTTGGACTGTGTTGTTGAGGTTATGAAAGAAGATGAAAAGAATGTTTGGATGTTTTGAGTAGGAGCAACATTGCTGCATTGGTTCAGTTGCTTACAGCATCATCAACAAGGATAAGAGAGAAACAGTTACCGTTATATGCTCGCTCGTCGAATCGGGTAGCTGCGAGGATTGGCTAGTTTCCGAAGGTGTTCTTCCGCCTCTTATAAGGCTCGTTGAATCAGGCAGTGCTGTAGGTAGAGAAAAGGCCGCGATATCGCTGCAAAGGCTATCAATGTCGGCTGAAACATCACGTG
TARGET=357,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3182
PRIMER_LEFT_PENALTY=0.130883
PRIMER_RIGHT_PENALTY=0.187326
PRIMER_LEFT_SEQUENCE=TTGATCAAAACTGGTATGCTCG
PRIMER_RIGHT_SEQUENCE=CTGTAAGCAACTGAACCAATGC
PRIMER_LEFT=321,22
PRIMER_RIGHT=569,22
PRIMER_LEFT_TM=60.131
PRIMER_RIGHT_TM=59.813
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.3182
PRIMER_LEFT_1_PENALTY=0.130883
PRIMER_RIGHT_1_PENALTY=0.187326
PRIMER_LEFT_1_SEQUENCE=TTGATCAAAACTGGTATGCTCG
PRIMER_RIGHT_1_SEQUENCE=ATGCTGTAAGCAACTGAACCAA
PRIMER_LEFT_1=321,22
PRIMER_RIGHT_1=572,22
PRIMER_LEFT_1_TM=60.131
PRIMER_RIGHT_1_TM=59.813
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.3182
PRIMER_LEFT_2_PENALTY=0.130883
PRIMER_RIGHT_2_PENALTY=0.187326
PRIMER_LEFT_2_SEQUENCE=TTGATCAAAACTGGTATGCTCG
PRIMER_RIGHT_2_SEQUENCE=GTAAGCAACTGAACCAATGCAG
PRIMER_LEFT_2=321,22
PRIMER_RIGHT_2=567,22
PRIMER_LEFT_2_TM=60.131
PRIMER_RIGHT_2_TM=59.813
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.3182
PRIMER_LEFT_3_PENALTY=0.130883
PRIMER_RIGHT_3_PENALTY=0.187326
PRIMER_LEFT_3_SEQUENCE=TTGATCAAAACTGGTATGCTCG
PRIMER_RIGHT_3_SEQUENCE=GCTGTAAGCAACTGAACCAATG
PRIMER_LEFT_3=321,22
PRIMER_RIGHT_3=570,22
PRIMER_LEFT_3_TM=60.131
PRIMER_RIGHT_3_TM=59.813
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.3182
PRIMER_LEFT_4_PENALTY=0.130883
PRIMER_RIGHT_4_PENALTY=0.187326
PRIMER_LEFT_4_SEQUENCE=TTGATCAAAACTGGTATGCTCG
PRIMER_RIGHT_4_SEQUENCE=TGCTGTAAGCAACTGAACCAAT
PRIMER_LEFT_4=321,22
PRIMER_RIGHT_4=571,22
PRIMER_LEFT_4_TM=60.131
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=251
=
PRIMER_SEQUENCE_ID=SSR_04924 Ps_contig_mira-and-tgicl-ass_franssen_35291
SEQUENCE=AGTACNCGGGGGTGCTCTCACTCACTTCTAGGTGGATTCTTAATCCTCCTTTTCTTTATTTCCCATTTTAACCCTGTTACTAGGGTTTCATTCTTCATCCCCATGGCTGAAGAGTACCGATGCTTCGTTGGTGGACTTGCTTGGGCCACCGACAACGACGCTCTCGGCAAAGCGTTTTCTTCCTTTGGCGAAATCGTCGAATCGAAGGTTATCCTTGATCGTGAAACTGGAAGATCGAGAGGATTTGGATTCGTGACCTTTGCATCGGAGCAATCAATGAAAGACGCCATTGAAGGGATGAACGGTCAGGATCTAGATGGCCGTAACATCACTGTGAACGAGGCTCAGAGCCGCGGCAGTGGTGGTGGTGGTGGAGGTGGATACGGTGGAGGCGGCGGTGGAGGATATGGAGGTGGCGGTGGTGGATATGRAGGAGATTGGAGGCTATGAAGGAGTATTGGAGGAGTTAATGT
TARGET=359,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04925 Ps_contig_mira-and-tgicl-ass_franssen_35297
SEQUENCE=GCATATTGAACAGCCTTGGGGAAGATTTCACCTTATTTGTATTAGAATGTGAGAATTCTCCAGCTAGATAGAGTCTGACTCGAACAAGTTTTGCTTCAGAAAATTTTCGCACGTTTTCCACCAAAGCAAATGCATGTGTAGTTGTGTCATCTACATACTTTTCTTTTGAAATCAGTAACAGATCATTCTGCGATATTGATTCTCCCTCTTCCAATTCACAAGGAAATTCTAGAAAATGAAAGTCATCTGACTCGGTGTAACTATTTACCACCCCAAACTTCCAATCAGCCCCCTGTTCTTCTTCTTCCTCTA
TARGET=296,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04926 Ps_contig_mira-and-tgicl-ass_franssen_35339
SEQUENCE=AGTTCAAAAGAGAGAGACGTTAGAAAAGAAAGAGAAAAAGCAGAACGGAAAAAACGAAGTGAATCAGAGAAGAAGAAAATGGCTACCAATATCGGAATAATGGACGCCGCGTACTTCGTCGGTAGATCTGAGATTCTCTCTTGGATCAATTCACTTCTTCAACTCAATCTCTCCAAAGTCGAAGAGGCTTGTTCTGGTGCTGTTCACTGTCAGCTTCTTGATGCCGCTCATCCTGGTATTGTTCCGATGCATAAGGTCAATTTTGATGCCAAGAGTGAGTATGAGATGATTCAGAACTATAAGGTTCTTCAAGATGTTTTCAACAAACTCAAAATCACTAAGCACATTGAGGTTAGTAAGCTAGTGAAAGGAAGACCGTTGGATAATCTGGAGTTCATGCAATGGATGAAACGATACTGCGATTCTGTCAATTCTGGACAGGACAGTTATAATCCTTTTGAGAGGAGAGAAGTATGCAAAGGAGCAAGAGAAGTGAGTAAAAGATCCGCACACTCTCAACCCTCAACTAAGGGTGGTTCCACTGCGGCACACAGGCCTCAATCTTCCCATGCTCGAAGAAATGATGTGTCTACTGTTAACCACTCTGCAAGTCATGGGGCTGCAAGGGTCTCAAGGTCTTCTGCCCCTGCTGCTGCTGTAAACCCTGCTTATGATCAACAGGTGCAACAGATAACAGAATTGAAGTTATCCATTGATAGCCTTGAGAAGGAACGTGATTTTTACTTTGCGAAGTTGAGGGACATTGAGATTCTTGGTCAGACTCCTGGAGTAGAAAACTCGCCGGTTTTTGAAGCAATCCTGAAGATATTGTATGCTACTGATGACAATGGATCTGAGTTGGCAGAAGCTCAAGCTATACTCGCTGCTGGCCTACAGGAAGCAAATACATTGAGTCCCATTGCTGAGGTTTCTGAAGAGAAGAGCAGTTCTAAGAGAAAGAATATCAACAATCCTGAATGTGATGCTGCTGGTGTCACTAATTTGTCTCCTAGACGGAGGCTTTCTGATATTTCAAATGTTCACTATGAAGGGTCACCTCTAGCATGACCTGAAAGAAAAAATTCAAGGAGGAAGAAAACTATTTTGGTTACAGAAATTTCAGATTTATGTGTTGTTACAATTGCTTTTTGTAATAATTAGGCGTCCTACAGAGATTATTATCTCATGAATATATGCTTATATATGCTTTTGATCCAGAAATATAGCTATGTCATAGTGGGTCTAATTTT
TARGET=647,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2270
PRIMER_LEFT_PENALTY=0.118120
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=TGCTCGAAGAAATGATGTGTCT
PRIMER_RIGHT_SEQUENCE=CTTCAGGATTGCTTCAAAAACC
PRIMER_LEFT=569,22
PRIMER_RIGHT=824,22
PRIMER_LEFT_TM=59.882
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=256
PRIMER_PAIR_PENALTY_1=0.2487
PRIMER_LEFT_1_PENALTY=0.139753
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=CATGCTCGAAGAAATGATGTGT
PRIMER_RIGHT_1_SEQUENCE=CTTCAGGATTGCTTCAAAAACC
PRIMER_LEFT_1=567,22
PRIMER_RIGHT_1=824,22
PRIMER_LEFT_1_TM=60.140
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.2770
PRIMER_LEFT_2_PENALTY=0.168114
PRIMER_RIGHT_2_PENALTY=0.108929
PRIMER_LEFT_2_SEQUENCE=TTAACCACTCTGCAAGTCATGG
PRIMER_RIGHT_2_SEQUENCE=CTTCAGGATTGCTTCAAAAACC
PRIMER_LEFT_2=595,22
PRIMER_RIGHT_2=824,22
PRIMER_LEFT_2_TM=60.168
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.3780
PRIMER_LEFT_3_PENALTY=0.117281
PRIMER_RIGHT_3_PENALTY=0.260719
PRIMER_LEFT_3_SEQUENCE=TAAAAGATCCGCACACTCTCAA
PRIMER_RIGHT_3_SEQUENCE=CTCCAGGAGTCTGACCAAGAAT
PRIMER_LEFT_3=497,22
PRIMER_RIGHT_3=789,22
PRIMER_LEFT_3_TM=59.883
PRIMER_RIGHT_3_TM=59.739
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.3788
PRIMER_LEFT_4_PENALTY=0.118120
PRIMER_RIGHT_4_PENALTY=0.260719
PRIMER_LEFT_4_SEQUENCE=TGCTCGAAGAAATGATGTGTCT
PRIMER_RIGHT_4_SEQUENCE=CTCCAGGAGTCTGACCAAGAAT
PRIMER_LEFT_4=569,22
PRIMER_RIGHT_4=789,22
PRIMER_LEFT_4_TM=59.882
PRIMER_RIGHT_4_TM=59.739
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_04927 Ps_contig_mira-and-tgicl-ass_franssen_35341
SEQUENCE=CTTTCACCTTTCATCAACTTAATGAAATCATTGAAGTCTAACAAGCTGTCGCCGTCGGCATCGAGATATTGAATCACCCTTTCAGCTTCTTCATGAGACATGTGCTCTCCAATTGACCCAAAATATGACCTTAATTCATAGGCTGAAATTTTTCCATCTCCATCACTATCAAAGTAATTGAAAACCTCTCTTAATCCCATATCATCTTCATTCTTGTTATTTTTTGGCGAGCATAATGATGATGATGATGAATAG
TARGET=236,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04928 Ps_contig_mira-and-tgicl-ass_franssen_35344
SEQUENCE=GATAGTAGTGGATACACCATAACACGATATCTCTATGCAACTTCAAACATCAACCTTCCTAACTTTTTTCTAGAAATCTAAGATCTCTTCTGTGTGGAGTACACATGCTAACTGGATGGGATACATCGCTGTCGCCACCGATGAAGAAGAAATTAAACGGTTAGGACGCCGCGACATAGTTATTGCATGGAGGGGCACAGTAACATACATAGAATGGATATACGACCTAAAAGACATTCTACACGAAGCAAACTTCAGAAATGACCCAACAATCAAAGTAGAATTAGGCTTCTATGACTTATACACTAAGAAAGAAGATTCATGCAGTTATTGCACATTCTCAGCTCGAGAACAGGTCCTCTCAGAAGTGAAACGCCTTGTACAATACTACGAAGAAGAAGAACTCAGCATCACAATAACAGGACACAGTCTCGGCGCTGCATTAGCCATACTCAGCGCTTACGACATCGCCGAACTAAACCTAAACGTCGTCAAAGATAGTAATAATAACATAACGAAAGTCATCCCAGTAACCGTTTACTCGTTCGCAGGCCCTAGGGTTGGGAATTTGAAGTTCAAAGAAAGGTGCGATGAACTCGGTGTTAAGGTTTTGAGAATATTAAACGTGCATGACAAGGTTCCAACCGTGCCTGGGATAATAACTAATGAGAAATTTCAGTTTCAGAGGTATATAGAAGATGCTTTGTCTTTTCCGTGGAGTTATGCACATGTTGGAACTGAAATCGCTTTGGATCATAGAGAAAGTCCGTTTTTGAAGGAAAATGGTGATTTGGGTTGTGCACATAATTTAGAGGTACTTTTGCATTTAATAGATGGGTATCATGGGAAGAAGAAGAAATTTGATTTGGTTTCGCAAAGAGATATTGCGCTTGTGAATAAGAGCTGTGATTTTCTACGGAGTGAGTATGGTGTACCGCCGCATTGGAGACAAGATGAGAATAAAGGGATGGTGAGATCCGGTGATGGACGGTGGGTTTTGCCTGAACGGCCACAATTGGAAGCTCATCCACCGGATACAGCACAACTTCTTGAGTTAGTGCTTAAATATCACATTCTGATTAATGCTAAGTTTCAATCAGGAGCAAAGTGATTTTGTTTTCTAGTAGTGAAAAAGGAAAGGATGGGGAAAAAAAAAACGAATGTGATAGATAAATAAATTGATAAGAATATATAACATG
TARGET=392,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1037
PRIMER_LEFT_PENALTY=0.080323
PRIMER_RIGHT_PENALTY=0.023406
PRIMER_LEFT_SEQUENCE=CACGAAGCAAACTTCAGAAATG
PRIMER_RIGHT_SEQUENCE=GTTTAGGTTTAGTTCGGCGATG
PRIMER_LEFT=241,22
PRIMER_RIGHT=486,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.023
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.1037
PRIMER_LEFT_1_PENALTY=0.080323
PRIMER_RIGHT_1_PENALTY=0.023406
PRIMER_LEFT_1_SEQUENCE=CACGAAGCAAACTTCAGAAATG
PRIMER_RIGHT_1_SEQUENCE=TTTAGGTTTAGTTCGGCGATGT
PRIMER_LEFT_1=241,22
PRIMER_RIGHT_1=485,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=60.023
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.1061
PRIMER_LEFT_2_PENALTY=0.082680
PRIMER_RIGHT_2_PENALTY=0.023406
PRIMER_LEFT_2_SEQUENCE=GCAGTTATTGCACATTCTCAGC
PRIMER_RIGHT_2_SEQUENCE=GTTTAGGTTTAGTTCGGCGATG
PRIMER_LEFT_2=323,22
PRIMER_RIGHT_2=486,22
PRIMER_LEFT_2_TM=59.917
PRIMER_RIGHT_2_TM=60.023
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.1061
PRIMER_LEFT_3_PENALTY=0.082680
PRIMER_RIGHT_3_PENALTY=0.023406
PRIMER_LEFT_3_SEQUENCE=GCAGTTATTGCACATTCTCAGC
PRIMER_RIGHT_3_SEQUENCE=TTTAGGTTTAGTTCGGCGATGT
PRIMER_LEFT_3=323,22
PRIMER_RIGHT_3=485,22
PRIMER_LEFT_3_TM=59.917
PRIMER_RIGHT_3_TM=60.023
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=163
PRIMER_PAIR_PENALTY_4=0.1125
PRIMER_LEFT_4_PENALTY=0.089133
PRIMER_RIGHT_4_PENALTY=0.023406
PRIMER_LEFT_4_SEQUENCE=GTTATTGCACATTCTCAGCTCG
PRIMER_RIGHT_4_SEQUENCE=GTTTAGGTTTAGTTCGGCGATG
PRIMER_LEFT_4=326,22
PRIMER_RIGHT_4=486,22
PRIMER_LEFT_4_TM=59.911
PRIMER_RIGHT_4_TM=60.023
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_04929 Ps_contig_mira-and-tgicl-ass_franssen_35344
SEQUENCE=GATAGTAGTGGATACACCATAACACGATATCTCTATGCAACTTCAAACATCAACCTTCCTAACTTTTTTCTAGAAATCTAAGATCTCTTCTGTGTGGAGTACACATGCTAACTGGATGGGATACATCGCTGTCGCCACCGATGAAGAAGAAATTAAACGGTTAGGACGCCGCGACATAGTTATTGCATGGAGGGGCACAGTAACATACATAGAATGGATATACGACCTAAAAGACATTCTACACGAAGCAAACTTCAGAAATGACCCAACAATCAAAGTAGAATTAGGCTTCTATGACTTATACACTAAGAAAGAAGATTCATGCAGTTATTGCACATTCTCAGCTCGAGAACAGGTCCTCTCAGAAGTGAAACGCCTTGTACAATACTACGAAGAAGAAGAACTCAGCATCACAATAACAGGACACAGTCTCGGCGCTGCATTAGCCATACTCAGCGCTTACGACATCGCCGAACTAAACCTAAACGTCGTCAAAGATAGTAATAATAACATAACGAAAGTCATCCCAGTAACCGTTTACTCGTTCGCAGGCCCTAGGGTTGGGAATTTGAAGTTCAAAGAAAGGTGCGATGAACTCGGTGTTAAGGTTTTGAGAATATTAAACGTGCATGACAAGGTTCCAACCGTGCCTGGGATAATAACTAATGAGAAATTTCAGTTTCAGAGGTATATAGAAGATGCTTTGTCTTTTCCGTGGAGTTATGCACATGTTGGAACTGAAATCGCTTTGGATCATAGAGAAAGTCCGTTTTTGAAGGAAAATGGTGATTTGGGTTGTGCACATAATTTAGAGGTACTTTTGCATTTAATAGATGGGTATCATGGGAAGAAGAAGAAATTTGATTTGGTTTCGCAAAGAGATATTGCGCTTGTGAATAAGAGCTGTGATTTTCTACGGAGTGAGTATGGTGTACCGCCGCATTGGAGACAAGATGAGAATAAAGGGATGGTGAGATCCGGTGATGGACGGTGGGTTTTGCCTGAACGGCCACAATTGGAAGCTCATCCACCGGATACAGCACAACTTCTTGAGTTAGTGCTTAAATATCACATTCTGATTAATGCTAAGTTTCAATCAGGAGCAAAGTGATTTTGTTTTCTAGTAGTGAAAAAGGAAAGGATGGGGAAAAAAAAAACGAATGTGATAGATAAATAAATTGATAAGAATATATAACATG
TARGET=847,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3197
PRIMER_LEFT_PENALTY=0.258908
PRIMER_RIGHT_PENALTY=0.060824
PRIMER_LEFT_SEQUENCE=GAGAAAGTCCGTTTTTGAAGGA
PRIMER_RIGHT_SEQUENCE=AAGAAGTTGTGCTGTATCCGGT
PRIMER_LEFT=758,22
PRIMER_RIGHT=1050,22
PRIMER_LEFT_TM=59.741
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=11.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.5179
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.410097
PRIMER_LEFT_1_SEQUENCE=AGAAGATGCTTTGTCTTTTCCG
PRIMER_RIGHT_1_SEQUENCE=AGCTCTTATTCACAAGCGCAAT
PRIMER_LEFT_1=693,22
PRIMER_RIGHT_1=904,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.410
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.5637
PRIMER_LEFT_2_PENALTY=0.258908
PRIMER_RIGHT_2_PENALTY=0.304841
PRIMER_LEFT_2_SEQUENCE=GAGAAAGTCCGTTTTTGAAGGA
PRIMER_RIGHT_2_SEQUENCE=TCAAGAAGTTGTGCTGTATCCG
PRIMER_LEFT_2=758,22
PRIMER_RIGHT_2=1052,22
PRIMER_LEFT_2_TM=59.741
PRIMER_RIGHT_2_TM=60.305
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.6230
PRIMER_LEFT_3_PENALTY=0.212871
PRIMER_RIGHT_3_PENALTY=0.410097
PRIMER_LEFT_3_SEQUENCE=CCGTGCCTGGGATAATAACTAA
PRIMER_RIGHT_3_SEQUENCE=AGCTCTTATTCACAAGCGCAAT
PRIMER_LEFT_3=644,22
PRIMER_RIGHT_3=904,22
PRIMER_LEFT_3_TM=60.213
PRIMER_RIGHT_3_TM=60.410
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.6422
PRIMER_LEFT_4_PENALTY=0.232060
PRIMER_RIGHT_4_PENALTY=0.410097
PRIMER_LEFT_4_SEQUENCE=GAAGATGCTTTGTCTTTTCCGT
PRIMER_RIGHT_4_SEQUENCE=AGCTCTTATTCACAAGCGCAAT
PRIMER_LEFT_4=694,22
PRIMER_RIGHT_4=904,22
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=60.410
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_04930 Ps_contig_mira-and-tgicl-ass_franssen_35346
SEQUENCE=GTTAAAGACCGAACAGAATGCAGATAAACATAACAAACGTTTAATTTATTGAATCTTCGAAGAAGCGAGTTGAAGAGTAAAGTATAGGTTTCGAGATTAGGTTTACAGTCTTGTGAGTTTAACATTTTGTTGTAGACGTCGAATGCGCGGTTGAAAAGGAACTTGCGGGAACAGCAGAAGCGGATGATGGAATTGTAGAGAGGGAGAGTGGGGTCGCAGGCGCCGGCGATGACTTCTTCGACTAGGGTTTCGGCTTGTTGGTAGCGGCGAGAGGCGGTGTAGGTGTTTGATGATGGTGAGGTAGGTGAGGTGGGTGTGTTTGTAGGAGCGTTGTTGGGAGGTCCAACGGAAAATGTCGAGGGCGAGGTCGGGGTCGGATTGGGAGGTTAAGGCGTTGTTGACGTCGGAAGGGGTGAAACCGGGTTTGAGATGGTTGACCCATGTTTCGAATTGTTTTTCGGAGGGGGGTTTTGGATGGGGATTGGAATGTTGGAATGGTGGTGGTGGAGAAGAGATAGGTGGAATGAAGAA
TARGET=496,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04931 Ps_contig_mira-and-tgicl-ass_franssen_35353
SEQUENCE=GTAAAGAAAATCAGACAACATATACGCTGATACAATTATAATTCAAATTTCACAAGAGTATAGTATTTTTCGTCTGTAAACAAATTGGAATTTAATTTACAGTAACAAACTGTCAAATCAGAAGACACCACATTCAAAAGTTATTAATCTACAAAAACAAATTCTCAATCTAACCTAACAAGTCAGTACATATATGATCATAGCAATGACCCGTAAGAGAGACTCCAGGCAGGTCCAGGGCAGAACATGACCCATACAGGTCAAACATAACTGTAAAATTCATGAATGTGAAGAAGCTAATGCAAATGCCCATAGCAATGCTCAGTTAAGCCATCATGCCTGCTTCTTCTTCTTAGGTCTTCTCTTTCTCCGGGGCTTGTGAGCATCAGAGTTGGAGCTTATACTACTTCCATGACTTGTCCCTGATCTTGAAGGTTCCCTTACTCTAAAGTCTGAGCTGCTTTTTCTGTTTGATGTGTAGTTTGATGTGTTGCTGGATGTGGAAATCTCAAATCTCGGTTTAGATGAAGTTTGACCTGAAGATGATCCAAACCTTCCCCTTACTCTGAAGTCTGAGCTGCTTTTGTTATTTGATGTATTGTTGAATTTAGAAAACTCAGATCTCTGTTTAGATGAATTTTGGCCTGAAGATGATCCAAACCTTGACATCTTGTTTGAAGGGCGAGGAGGAGGCATCAAAGTTCTAGCAGAAATCTGTCTCTGATTTTTTTCATCGCTGGACATGCCAGACACAGCTTTTCCAGAAGCACTTGAAGCCTGCTCTCTGGTGCTTTTGTCTGTCAATTCATCATCTGCATCAGCTTCATCACTAGAACTTCCAGCAAGGAAAAAATCATCATCACTGAGATCTAAAAGCCCGTCATCCTCGCTGCCAGTCTCTTCTAAATCCTTGCCACTTGCAGCAGCTGCAGATAACCTTTCTTCAATCTGTTTGCGCAGCCCATTTCGCTTGTCAACTATCTCTTGATCATCACTTCCACAAAATAAAGGAACATATTTCTCATTCTTGGGAAAGTACATAACATATTGAAGATCATGTTTAAGAGATGCAAGCTGGTCAGGGGAAGAAGAAGACGAAGCACGCTGAAGCTTCTCTAGGCGTCTAATTCTCCGTTCAATTTTCCTCCTCTCAAAGAACTTAATCTTCTTGTCGCGCAAAAATAGCTTCCGTTCCAAAGCTAGACGGGTGTGAACCTCCTGCTGTTTCTTCAGTGATTCCAGCTTCTGCTCCTGTGCTGCTTTAACTTCCAACGGTAAATTCTTTCGGAGCATGCGTTCTGCGGATCTAATCTGGTTTTTGAGTGACACCGGAGGTTTGAATTTCTTGGTGGTGGATCGACGGCTTGCGGGGTTAACCCTCACGCGCCGCTTGTTTAAACCACCGTGAACCATGTCGCCGCTGTGTATTTTGCTTCCTCGGCGGTTTAGGTTTTCTGATTCTCTATTATTTTCTTCACCTCTTTTGATGTATAAACACGGCGGAACTTGAGGGAGTAATGCTTTTCCAATTAAATTCGAGTATATTTTATTTTATTTTTGGCTTTAATNGATGGATGACTGAAAACTAGGGTTTTGAACACCCCAAAATAT
TARGET=343,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0671
PRIMER_LEFT_PENALTY=0.016312
PRIMER_RIGHT_PENALTY=0.050763
PRIMER_LEFT_SEQUENCE=TAAGAGAGACTCCAGGCAGGTC
PRIMER_RIGHT_SEQUENCE=CTCCAACTCTGATGCTCACAAG
PRIMER_LEFT=213,22
PRIMER_RIGHT=396,22
PRIMER_LEFT_TM=60.016
PRIMER_RIGHT_TM=60.051
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.0902
PRIMER_LEFT_1_PENALTY=0.016312
PRIMER_RIGHT_1_PENALTY=0.073900
PRIMER_LEFT_1_SEQUENCE=TAAGAGAGACTCCAGGCAGGTC
PRIMER_RIGHT_1_SEQUENCE=CACATCAAACAGAAAAAGCAGC
PRIMER_LEFT_1=213,22
PRIMER_RIGHT_1=477,22
PRIMER_LEFT_1_TM=60.016
PRIMER_RIGHT_1_TM=59.926
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.1825
PRIMER_LEFT_2_PENALTY=0.131699
PRIMER_RIGHT_2_PENALTY=0.050763
PRIMER_LEFT_2_SEQUENCE=ATGACCCGTAAGAGAGACTCCA
PRIMER_RIGHT_2_SEQUENCE=CTCCAACTCTGATGCTCACAAG
PRIMER_LEFT_2=205,22
PRIMER_RIGHT_2=396,22
PRIMER_LEFT_2_TM=60.132
PRIMER_RIGHT_2_TM=60.051
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=192
PRIMER_PAIR_PENALTY_3=0.2056
PRIMER_LEFT_3_PENALTY=0.131699
PRIMER_RIGHT_3_PENALTY=0.073900
PRIMER_LEFT_3_SEQUENCE=ATGACCCGTAAGAGAGACTCCA
PRIMER_RIGHT_3_SEQUENCE=CACATCAAACAGAAAAAGCAGC
PRIMER_LEFT_3=205,22
PRIMER_RIGHT_3=477,22
PRIMER_LEFT_3_TM=60.132
PRIMER_RIGHT_3_TM=59.926
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.2096
PRIMER_LEFT_4_PENALTY=0.016312
PRIMER_RIGHT_4_PENALTY=0.193337
PRIMER_LEFT_4_SEQUENCE=TAAGAGAGACTCCAGGCAGGTC
PRIMER_RIGHT_4_SEQUENCE=TCAAACAGAAAAAGCAGCTCAG
PRIMER_LEFT_4=213,22
PRIMER_RIGHT_4=473,22
PRIMER_LEFT_4_TM=60.016
PRIMER_RIGHT_4_TM=59.807
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=261
=
PRIMER_SEQUENCE_ID=SSR_04932 Ps_contig_mira-and-tgicl-ass_franssen_35353
SEQUENCE=GTAAAGAAAATCAGACAACATATACGCTGATACAATTATAATTCAAATTTCACAAGAGTATAGTATTTTTCGTCTGTAAACAAATTGGAATTTAATTTACAGTAACAAACTGTCAAATCAGAAGACACCACATTCAAAAGTTATTAATCTACAAAAACAAATTCTCAATCTAACCTAACAAGTCAGTACATATATGATCATAGCAATGACCCGTAAGAGAGACTCCAGGCAGGTCCAGGGCAGAACATGACCCATACAGGTCAAACATAACTGTAAAATTCATGAATGTGAAGAAGCTAATGCAAATGCCCATAGCAATGCTCAGTTAAGCCATCATGCCTGCTTCTTCTTCTTAGGTCTTCTCTTTCTCCGGGGCTTGTGAGCATCAGAGTTGGAGCTTATACTACTTCCATGACTTGTCCCTGATCTTGAAGGTTCCCTTACTCTAAAGTCTGAGCTGCTTTTTCTGTTTGATGTGTAGTTTGATGTGTTGCTGGATGTGGAAATCTCAAATCTCGGTTTAGATGAAGTTTGACCTGAAGATGATCCAAACCTTCCCCTTACTCTGAAGTCTGAGCTGCTTTTGTTATTTGATGTATTGTTGAATTTAGAAAACTCAGATCTCTGTTTAGATGAATTTTGGCCTGAAGATGATCCAAACCTTGACATCTTGTTTGAAGGGCGAGGAGGAGGCATCAAAGTTCTAGCAGAAATCTGTCTCTGATTTTTTTCATCGCTGGACATGCCAGACACAGCTTTTCCAGAAGCACTTGAAGCCTGCTCTCTGGTGCTTTTGTCTGTCAATTCATCATCTGCATCAGCTTCATCACTAGAACTTCCAGCAAGGAAAAAATCATCATCACTGAGATCTAAAAGCCCGTCATCCTCGCTGCCAGTCTCTTCTAAATCCTTGCCACTTGCAGCAGCTGCAGATAACCTTTCTTCAATCTGTTTGCGCAGCCCATTTCGCTTGTCAACTATCTCTTGATCATCACTTCCACAAAATAAAGGAACATATTTCTCATTCTTGGGAAAGTACATAACATATTGAAGATCATGTTTAAGAGATGCAAGCTGGTCAGGGGAAGAAGAAGACGAAGCACGCTGAAGCTTCTCTAGGCGTCTAATTCTCCGTTCAATTTTCCTCCTCTCAAAGAACTTAATCTTCTTGTCGCGCAAAAATAGCTTCCGTTCCAAAGCTAGACGGGTGTGAACCTCCTGCTGTTTCTTCAGTGATTCCAGCTTCTGCTCCTGTGCTGCTTTAACTTCCAACGGTAAATTCTTTCGGAGCATGCGTTCTGCGGATCTAATCTGGTTTTTGAGTGACACCGGAGGTTTGAATTTCTTGGTGGTGGATCGACGGCTTGCGGGGTTAACCCTCACGCGCCGCTTGTTTAAACCACCGTGAACCATGTCGCCGCTGTGTATTTTGCTTCCTCGGCGGTTTAGGTTTTCTGATTCTCTATTATTTTCTTCACCTCTTTTGATGTATAAACACGGCGGAACTTGAGGGAGTAATGCTTTTCCAATTAAATTCGAGTATATTTTATTTTATTTTTGGCTTTAATNGATGGATGACTGAAAACTAGGGTTTTGAACACCCCAAAATAT
TARGET=1543,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2364
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=1.140999
PRIMER_LEFT_SEQUENCE=TTCTGCGGATCTAATCTGGTTT
PRIMER_RIGHT_SEQUENCE=CAGTCATCCATCNATTAAAGCC
PRIMER_LEFT=1296,22
PRIMER_RIGHT=1580,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=58.859
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=1.5109
PRIMER_LEFT_1_PENALTY=0.369874
PRIMER_RIGHT_1_PENALTY=1.140999
PRIMER_LEFT_1_SEQUENCE=CTGCGGATCTAATCTGGTTTTT
PRIMER_RIGHT_1_SEQUENCE=CAGTCATCCATCNATTAAAGCC
PRIMER_LEFT_1=1298,22
PRIMER_RIGHT_1=1580,22
PRIMER_LEFT_1_TM=59.630
PRIMER_RIGHT_1_TM=58.859
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=283
PRIMER_PAIR_PENALTY_2=1.5209
PRIMER_LEFT_2_PENALTY=0.379939
PRIMER_RIGHT_2_PENALTY=1.140999
PRIMER_LEFT_2_SEQUENCE=GTTCTGCGGATCTAATCTGGTT
PRIMER_RIGHT_2_SEQUENCE=CAGTCATCCATCNATTAAAGCC
PRIMER_LEFT_2=1295,22
PRIMER_RIGHT_2=1580,22
PRIMER_LEFT_2_TM=59.620
PRIMER_RIGHT_2_TM=58.859
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=1.5873
PRIMER_LEFT_3_PENALTY=0.446308
PRIMER_RIGHT_3_PENALTY=1.140999
PRIMER_LEFT_3_SEQUENCE=CTTTTGATGTATAAACACGGCG
PRIMER_RIGHT_3_SEQUENCE=CAGTCATCCATCNATTAAAGCC
PRIMER_LEFT_3=1480,22
PRIMER_RIGHT_3=1580,22
PRIMER_LEFT_3_TM=59.554
PRIMER_RIGHT_3_TM=58.859
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.50
PRIMER_LEFT_3_END_STABILITY=13.4000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.75
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=1.5982
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=1.502777
PRIMER_LEFT_4_SEQUENCE=TCTGCGGATCTAATCTGGTTTT
PRIMER_RIGHT_4_SEQUENCE=CCCTAGTTTTCAGTCATCCATCN
PRIMER_LEFT_4=1297,22
PRIMER_RIGHT_4=1590,23
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=60.503
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.5500
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_04933 Ps_contig_mira-and-tgicl-ass_franssen_35354
SEQUENCE=CACAGCAATTGCCCCAGCCTCAACTAAAGTACTTAAGTGCCATGTCATCAATTCCTTTAGTAGTCAAAACAACATTGGCGCCAGCTTTGAGTAGTTTTTGAATCCGTTCCTTAGTCATATCAGCCTCTCTTTGGCGAATTCTCTCAAGTTCCTTGGGATCAGAAACTAATACTTGAACACCCAGTTGCATTTTTGTCTTCTGAAGATTGAAATCAAGACAAGCAATTTTTGCAGGGGAAACCTTGAGAGGCATTCCTTGGGCAGCACGGCCAGTATTAAGAGCATAACCATTCATCAAAAAGCTCTCTCTAGCACTTTTACCGTGTGCCTTCAATATATTGATTCCCTTGATTGGGTATCTAATTTCACCGCGAGCATTGGTCATCTTTACAGCTTGCACTGCATCCACAACCAAATTCGCAAAGAAGTCACTGTCGCCAGCTATCAGCTTCGAGGACATGCTGGTCTTGGCACAGTTAACTAGTGAATCTTTTCCTAGCTTTTCAACCTTGACAGCCAGTTTTTCATCAATGTATTTGCACGCCTCTCGCATAGCAAGCCTATATCCACTGATAATTGAGGTTGGATGAATCTTATTCCTAACAAGATCGTTTGCTCTTTTAAGAAGCTCTGCAGCTACAATGACAACTGAAGTAGTACCATCTCCAACTTCTCTATCCTGAAGTTCAGCCAGCTCAACCAAAACCTTAGCAGCAGGATGTTCAACTTCCAGCATCTTGAGAATGGTAGCGCCGTCGTTAGTGATAGTAACATCACCGATGTCATCGACAAGCATCTTGTCGAGACCGACGGGACCTAATGAGGTTTTGACGATGTTTGCAACGGCTTGGCATGCTACAACGTTTTGGGTGCGAACGTCTTGGCCGGATTGTCGTTCGCCGGAGATGTCAGGGGTTTGATTAACAAGCGCCATTGCGAAGTGTATGTGCGTGTCTAGGGCTTTCCACTGACTCACTCTGATAGTTTACACTACTACGAATTAAGGGTTTTGTTTTTCACTTGACCTACCTTACTTCTTTTCTTTATGTTTTTTATTTTTATGCTTCCTTCTCTCTTCCTTTCTATTTCATTTCATTTCATTGTAAAATGATTTTAATTCCCCGAC
TARGET=1085,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04934 Ps_contig_mira-and-tgicl-ass_franssen_35356
SEQUENCE=CGGGGACTCATTCACTTCTCATTCTCTCTCTCATACTGCATTTTGTCCCTCCTTTCATCAACCCTACAAAATCAAAGAAAAGATGGTTCCAATAAGTTTGCTCTTGGTTGGATTTCTTACTATATTTTCCTCAGCTTATGCCTATGGAGGAGGATGGATTAATGCACATGCAACCTTCTATGGAGGAAGTGATGCATCAGGAACAATGGGTGGGGCATGTGGATATGGTAACCTATACAGTCAAGGTTATGGAACAAACACAGCTGCTTTAAGCACAGCTTTGTTCAACAGTGGCTTAAGCTGTGGGTCTTGTTTTGAGATAAGATGTGCTGGTGACCATAAATGGTGTCTTCCTGGTTCCATTTTGGTTACTGCCACTAATTTCTGTCCTCCAAACAATGCCTTACCTAATAATGCTGGTGGATGGTGTAACCCTCCTTTGCATCACTTTGATCTTGCTCAACCTGTTTTTCTAAGAATTGCTCAATATAAAGCTGGAATTGTTCCTGTTTCTTACAGAAGGGTACCCTGCAGGAGAAGGGGAGGAATTAGGTTTACAATAAATGGACATTCCTACTTCAATTTAGTCCTAGTAACAAATGTGGGTGGTGCTGGAGATGTACATTCAGTTGCTATTAAAGGTTCAAGAACAGGGTGGATGCCAATGTCAAGAAACTGGGGACAAAATTGGCAAAGCAACAATTATCTTAATGGACAACCCTTGTCTTTTAAGGTTACAACAAGTGATGGAAAAACCATTGTTTCAAACAATGTTGCCCCTTCTGGTTGGTCCTTTGGTCAAACCTACACCGGTGCTCAATTCCACTAGACACATAATTTATCATCATATATAATACAATTTATATATAGTATGTTTTAATTTAATTTCCTAAGTTAAGTATTAGTATAACTCAATAGTATACTAGGGTATTAAATAGTATGATGAGTTATTGAAATTGAAATTGAAATTCTAGGGTTAGAAAATGTGAAGGCCTTTTTTTTGAATTGGCTTTTCATCATTTTGATTGTAGCAAGATGGTTCATATTTATTTGGGCCATTTTGATTATTGGGGTATTTTTTTCTTTTGAATTAATCTTCTGTTGCTAAAAAGCTTACAGAGATTAATAATTATTANGGTATTTTATAA
TARGET=23,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5817
PRIMER_LEFT_PENALTY=0.557021
PRIMER_RIGHT_PENALTY=0.024665
PRIMER_LEFT_SEQUENCE=GGGGACTCATTCACTTCTCATT
PRIMER_RIGHT_SEQUENCE=CATAGAAGGTTGCATGTGCATT
PRIMER_LEFT=1,22
PRIMER_RIGHT=181,22
PRIMER_LEFT_TM=59.443
PRIMER_RIGHT_TM=60.025
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.6874
PRIMER_LEFT_1_PENALTY=0.557021
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=GGGGACTCATTCACTTCTCATT
PRIMER_RIGHT_1_SEQUENCE=CACTTCCTCCATAGAAGGTTGC
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=190,22
PRIMER_LEFT_1_TM=59.443
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.7443
PRIMER_LEFT_2_PENALTY=0.557021
PRIMER_RIGHT_2_PENALTY=0.187326
PRIMER_LEFT_2_SEQUENCE=GGGGACTCATTCACTTCTCATT
PRIMER_RIGHT_2_SEQUENCE=AGCAGCTGTGTTTGTTCCATAA
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=59.443
PRIMER_RIGHT_2_TM=59.813
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=0.7443
PRIMER_LEFT_3_PENALTY=0.557021
PRIMER_RIGHT_3_PENALTY=0.187326
PRIMER_LEFT_3_SEQUENCE=GGGGACTCATTCACTTCTCATT
PRIMER_RIGHT_3_SEQUENCE=AAGCAGCTGTGTTTGTTCCATA
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=268,22
PRIMER_LEFT_3_TM=59.443
PRIMER_RIGHT_3_TM=59.813
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.8639
PRIMER_LEFT_4_PENALTY=0.557021
PRIMER_RIGHT_4_PENALTY=0.306868
PRIMER_LEFT_4_SEQUENCE=GGGGACTCATTCACTTCTCATT
PRIMER_RIGHT_4_SEQUENCE=ATGTGCATTAATCCATCCTCCT
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=168,22
PRIMER_LEFT_4_TM=59.443
PRIMER_RIGHT_4_TM=59.693
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=168
=
PRIMER_SEQUENCE_ID=SSR_04935 Ps_contig_mira-and-tgicl-ass_franssen_35357
SEQUENCE=TTTAACAAGCAACAACAGATACATATAACCGGAACCAAGGATTTACAAAGCTCAGCTGTTACAGTGACAAGGAATTTGACAAGACAAGTTGGGAATCAGGATTCATAAAGGAGACATAAATGAGCTAACCAAAACCCATCAAAGAATTGAAAAGTACTCACACTAATCACATACGGAAGCGACCAATAAGCCTGCATTGTTCTGGAAGTAAAGAAGAAATTATTTGTCCATTAAGTGCAAAAAGCTCACCATCAATCCCACATCCGCTGTGAGTGTGTTTTCCAGACTTTATTCTGACAGATTTTACCTTCACATATTCAACATATGGCAGTGAAAGGTGTTGGCCCATCTGTAGAAGTAAAAAAAATACTCAACAGCCTCAGCCTTCCACTCCCATGAACCATGATCAGATCTAGAGTGTTATCGTCGTGCTCAGCCTTGGGAGCAACTACCTGGGAGCTCTGAACAGTTCTACAAGCATGGTTGCAAACCAGAATGCCAACAAACTGTCCCTTTGACACAACCCATTTCTCTCCAAAATGAGGCACAGCCTCCTTCGTAGATCCTACTTCGGTATCATCTGGTGGCCCTGGCAACTCAATGGGGTTTTCCGCATCCCAATTAGCCTCTGCATCCCATTTTGGTTCAGCATCCCATATAGGACCTGGGTCAGACAGTGTAGATGATATATCCTCTCTATCATTTGTTGTATTCCCCCATTTAGAGGTATCATGTGTGGTGGTCAATCCAGTCCATCCTCTATCATTCCTTGACTTAGATCTGGTTCGTGGCCAGTTTGGAGTTGTAGACAGAGGCAGCTGTGGATGGATGACTTCTGGCTCTGATGTGATATTGCTCCTTCCAGATGATAGACGTTTTGACTTTGGATCTAAGCCACGCACCAATTCAGAAGGTTCAGTGCTAGCATGAGTGCTACTGCAGGTATCCAGATCTCCACCAGATATCCGACTCGGAGTCATTATCGAGTCGATACTTGAAAGACTAGATGCCCTGGGCAATCCATCCTTATTTGATCGACCCATGATGTCAGTATACAGCTCCGATATGTCTACTACTTCCTTTTCACCAGAAAGCTTTCCTTCTCTCTCAGCTTTTGATGCTGGAAGATATTCAACTTCATAGCTGTATCGCGGTAAGCATAAGAACTTGAGAAATCCAGCAACAAAATACCGCAGTGGGCCAAAGCGCTTCTGATATTTTTCAGAAAGTTCCAGCACATCACTAACAAAACCATAATATTGAGACTGTTAATCCAAAGTGAATTTTATTATTCTGAATCCACTCAACAGCAAAGACATCTGTAGCGGTAAGGCCCCCCTTCACAATAGCCATTGCCGCAGAAATCGGATCCCTAACTCCTAGAACAGTCCATACCAGTGAGTTATCAGAACCAGCTGGTATAATTCCAATAGGTATAGATATTCCTTCCTTTTGATTGTCTCTACTAAGGAGACCATTAACAACCTCATTGATTATCCCATCACCACCAACACATATAATACCATCAGGGCAAGTGCTGATGTCTACAGTGGATGCAAGATTTCTGGCATGACCAGCAGATGTTGTTTTGACCACCTCCAATCTAAACCCAGCAAGCCTAAAAATTGGCTCCACGGTATCATGGAAAACTTTACTTGAACGACCTCTGCCTGAGCGTGGGTTCAAAATTACAAGCATCTTGGGTGGAGTTTTGCATCTGAAGAGCAATTCAGGAGGGGTATCGGTTTGAAACAATTCCGAGGACGCTTGCTTCTTGGAAGACACTAAAGGGTGAGGAAGACAGTTAACGAAACAGTGTTGATCAGCAAAACCACCAACCCACTGAATTGCCTCTTCTATTGTAGTAGCCACAAAACGAAAGTCCTTGCGACTCCTTCGAGATTTAATAAAACAAGAAAGACCACATGAAGCCTTTTTCATTGGATACGAGTGCACTGTGAAATGTTTAAGACCAGCATGATAAGAAACCGAGATGACATCGTCAAGGTGCAGCAGATGAGATCCCCAAAGTAAAGCTTTGCTTGTTAGTTTGGCATCCACAGAGGCTTGGTTGGTAGTGTCAAAAGAAGTTTGTTGAGCATCACTTTTATTGTTATTTACAGAAATCTTCCTCTTATCCAAAACCAATTTTCCAGAGAATACTACATACCCTAACAAATCAGACTTCTCATCTCCTCCACCACCACCACCACCTCCTATGTCAATCCTATGCTCAAAATTCTTAGCCGCGGCATCCTGATCAGCACCACCTCCCGGCCTAACCGCATCAGTAGCCTTCTTGGAAGCCTTGAGTTTACCACGTTTTTCAGGGAAGACAATAGGAGAAGAGTGTTCCCCGGAAGTAGAAATCTGGGAACACAATCCTAAACGTCTGAAAGACTGTTGAGGTGAAGAGAGTCTAAGTGCGGCGGCGGCAGCAGCGGAATAGCAGCAGACTTAGAGGTGGTGTTACTAC
TARGET=2198,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1079
PRIMER_LEFT_PENALTY=0.041548
PRIMER_RIGHT_PENALTY=0.066394
PRIMER_LEFT_SEQUENCE=AAGCTTTGCTTGTTAGTTTGGC
PRIMER_RIGHT_SEQUENCE=AGAAGGCTACTGATGCGGTTAG
PRIMER_LEFT=2033,22
PRIMER_RIGHT=2299,22
PRIMER_LEFT_TM=59.958
PRIMER_RIGHT_TM=59.934
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.1347
PRIMER_LEFT_1_PENALTY=0.041548
PRIMER_RIGHT_1_PENALTY=0.093130
PRIMER_LEFT_1_SEQUENCE=AAGCTTTGCTTGTTAGTTTGGC
PRIMER_RIGHT_1_SEQUENCE=CCCTGAAAAACGTGGTAAACTC
PRIMER_LEFT_1=2033,22
PRIMER_RIGHT_1=2331,22
PRIMER_LEFT_1_TM=59.958
PRIMER_RIGHT_1_TM=59.907
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=299
PRIMER_PAIR_PENALTY_2=0.1620
PRIMER_LEFT_2_PENALTY=0.095574
PRIMER_RIGHT_2_PENALTY=0.066394
PRIMER_LEFT_2_SEQUENCE=AGCAGATGAGATCCCCAAAGTA
PRIMER_RIGHT_2_SEQUENCE=AGAAGGCTACTGATGCGGTTAG
PRIMER_LEFT_2=2011,22
PRIMER_RIGHT_2=2299,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=59.934
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.1620
PRIMER_LEFT_3_PENALTY=0.095629
PRIMER_RIGHT_3_PENALTY=0.066394
PRIMER_LEFT_3_SEQUENCE=ACAGAGGCTTGGTTGGTAGTGT
PRIMER_RIGHT_3_SEQUENCE=AGAAGGCTACTGATGCGGTTAG
PRIMER_LEFT_3=2059,22
PRIMER_RIGHT_3=2299,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.934
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=0.1888
PRIMER_LEFT_4_PENALTY=0.095629
PRIMER_RIGHT_4_PENALTY=0.093130
PRIMER_LEFT_4_SEQUENCE=ACAGAGGCTTGGTTGGTAGTGT
PRIMER_RIGHT_4_SEQUENCE=CCCTGAAAAACGTGGTAAACTC
PRIMER_LEFT_4=2059,22
PRIMER_RIGHT_4=2331,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=59.907
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_04936 Ps_contig_mira-and-tgicl-ass_franssen_35366
SEQUENCE=AACTTTTCTGTTTTTCACAGATCAATTTCTGTCCTGCATTTTACAAACAAGAACAGTATTTTTTATTTTATATAGTTCATTCCTCTTACAGCACACTTGAAAATTGACGATAGCAAACAAACAAAACAACAAAGGCACAAGAAACATCCATAAATGTCTTAATTAGGTATCATGGCAAGAACTTTAGACCAAGCTGGTCTTGCAGTAATATCAGCAACCCAAGCACTAACATGAGGTCGTGACTCAAACATCTTCTTGCTCTGTGACTTCATCAAGTAATGAAGAGAAGGTAAGTGATGCAGATCCACCAATGTGAAGCACTCACCTCCCAAGTATTTTGACTCTGACAATCTTTTCTCATACACATCAAGAATACTATCCAACTTCCCTTCATTTTCTTCCACAACTTTTGGATCCGTAGGCTTTCCAAATAAAGGCTTTATCCCAAATTCTGTAACAAGTTTTGATGCTACTTGATCGTAGTGTTGGCTCTCCACTTCCGACCATACTCCAATTATTGCCTTCTTCTTTGAGTCAGTGCTTGTTAACTTCGTCCCTTTATCAGCGTACTCGTGATTAATATATTGTGTAATAGCCCTTGATTCAAAGAGCTTTAAATCTCCGTCTTCAAAAGCTGGAACTTGACCAAATGGATGGAGTGATATGAATGGTTCCTTTTTGTGTTCACCATTTTTGAGGTTAATGTAAACGAACTCAAATTCAAGCTGTTTTTCATACAGAGTCGCTGTAACCCGCATGGTTGCTGTTGAGAAGGGGCTTCCGTGCACTTTTATTGTTGTCATGATAAAGATTTAGCAGAATCAGAAGATGTGTTTAGTAGTAATGGTATTTTGTAGTATCATCTCTAATCA
TARGET=114,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2110
PRIMER_LEFT_PENALTY=0.210973
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=ACAGCACACTTGAAAATTGACG
PRIMER_RIGHT_SEQUENCE=TTGGTGGATCTGCATCACTTAC
PRIMER_LEFT=87,22
PRIMER_RIGHT=310,22
PRIMER_LEFT_TM=60.211
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.2597
PRIMER_LEFT_1_PENALTY=0.259704
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=CACAGATCAATTTCTGTCCTGC
PRIMER_RIGHT_1_SEQUENCE=TTGGTGGATCTGCATCACTTAC
PRIMER_LEFT_1=15,22
PRIMER_RIGHT_1=310,22
PRIMER_LEFT_1_TM=59.740
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=0.2827
PRIMER_LEFT_2_PENALTY=0.210973
PRIMER_RIGHT_2_PENALTY=0.071684
PRIMER_LEFT_2_SEQUENCE=ACAGCACACTTGAAAATTGACG
PRIMER_RIGHT_2_SEQUENCE=ACCTCATGTTAGTGCTTGGGTT
PRIMER_LEFT_2=87,22
PRIMER_RIGHT_2=236,22
PRIMER_LEFT_2_TM=60.211
PRIMER_RIGHT_2_TM=59.928
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=0.2907
PRIMER_LEFT_3_PENALTY=0.210973
PRIMER_RIGHT_3_PENALTY=0.079714
PRIMER_LEFT_3_SEQUENCE=ACAGCACACTTGAAAATTGACG
PRIMER_RIGHT_3_SEQUENCE=GCAAGAAGATGTTTGAGTCACG
PRIMER_LEFT_3=87,22
PRIMER_RIGHT_3=258,22
PRIMER_LEFT_3_TM=60.211
PRIMER_RIGHT_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.3314
PRIMER_LEFT_4_PENALTY=0.259704
PRIMER_RIGHT_4_PENALTY=0.071684
PRIMER_LEFT_4_SEQUENCE=CACAGATCAATTTCTGTCCTGC
PRIMER_RIGHT_4_SEQUENCE=ACCTCATGTTAGTGCTTGGGTT
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=236,22
PRIMER_LEFT_4_TM=59.740
PRIMER_RIGHT_4_TM=59.928
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_04937 Ps_contig_mira-and-tgicl-ass_franssen_35367
SEQUENCE=GGCATGTTAAAGTTGATGAATCTATTTCATCGGTTCTTCTGAATGAGAATATACACACCATGTCCTGGCTCAACAATCATATTGTAAGCAGGAGAATGCTTATAACTAGGAGACAAAGAGAAGCTGAACTTAGAGACAATAAGGGCTAGCACAACCTTTAGTTCAACCATTGCAAAGTTCTTCCCCACACACAATCTAGTGCCTATTCCAAATGGCACATAGGCTTGTGGAAACTTGATAGCCTTAGAAACACCCTCACTGAACCTTTCTGGGTTAAACTCATTTGAATCTGGTCCCCAAATTTCAGGATCTCTGTGCAAAGTTGGTATCAGTGTCCATAAACAAACACCTTTTGGAACATTTAGGCTTCCAATTTGGATATCTTCATATGCTTCCCTTGATACAAAGGCTGCTGGTGGATATAATCTTAGCACTTCTTGAATCACCATAGTCACCGTTTTCAAAAGAGGGAGAGAATCTGCATCTGGTATGCCATTGGGGCAATGTTGAGCAACCTCAGTCCTAATACGAGTTTGCCATTCGGGGTACAAAGCAAGAAGCATCAAACACCAAGAAGCTGCAACAGCAGTAGTTTCATGACCAGCAAAGTATATGTTTTTGCAGTTATCCACAATGAACTGCTTTGAGAAATCCTTCCCCAAACTTTGATCACTCATTGCTGCTTCCAGCAGTAACTGCATCAAATCCTTCTCTGACGAGGATTCTCGCTTCCGTTCCTCCACTAACTCCCATATCAGTGACTCTATCTCTTTCTCCAAACTACTAATCTCATTTTCCTTCTTCGTCCGAAATTTCAGTTTCTCCAGGAAACCGCTCTGTCCAAAAAGGAAGCCATGGTTTGACATGATCTTTTGGATGCTTCTAAGCTTCAAGAAAACTTCTTTTCCCTTAGAATAAGAATGTCCAAAACAAACCCTTGAAATAACATCAGCTGAAAAGCCTCTCAAATCAGCATCAACTTTAACTTCTGCTGTGGCACCACCACCTTGCTCCTCAATAATTTGTTCCCATTTTAGAAGTAATGGCTGTGCTGACTCTATCATAAGCCCCACCATACCCTTAACTTTGTCCATGAAAAACTCAGCTGCAACAAGCTTTCTCTGTTGTGCCCAACTATGACCATTGGCTCTCAAAATCCCATTACCAAGCATTGGTGAAAGTTTGTTAGTAATATAAGAAGGCTTACCTAAACTCAAAGTGATGCATTGGTTCATTTCCTTCACGAGATCCGGTTCGTTTACGTAAAGGTGTTGCTTCATCCCTATCGAGTAAGTGTACACTAAACCGTATTGTTTCCTCCACTGTTGAAAAAATGGGAAGAGTGCTGCAGAATAATCATGAGCTAGAAACTGATCATAATTTGGTTTGGATAAGCTAGCTTGTGATTTGATTCTCTGCATATCAGGTAGATTCCCATGTAGAAAAGAAGAAGGTGGAGGCCCTTTAATACCTTGCATTTGGAGCTTCCTTCTAACCCTTTGAGATTTATACCACAAATTGCCATAAACATAAATTATCCAGCTGAAAATACCTACAACAGCAAGTGAAGAAGCTAGTTTCAAACCAAAAGAAAACACTTCCTCCATTTCTATTCCTCTCCACTCTCTCTCTCTTTGACTCTAACACAGTGCTTGCTTNCTT
TARGET=1623,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.8791
PRIMER_LEFT_PENALTY=0.129072
PRIMER_RIGHT_PENALTY=1.749986
PRIMER_LEFT_SEQUENCE=GCCCTTTAATACCTTGCATTTG
PRIMER_RIGHT_SEQUENCE=AAGCAAGCACTGTGTTAGAGTCA
PRIMER_LEFT=1459,22
PRIMER_RIGHT=1657,23
PRIMER_LEFT_TM=59.871
PRIMER_RIGHT_TM=59.250
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=1.9491
PRIMER_LEFT_1_PENALTY=0.199067
PRIMER_RIGHT_1_PENALTY=1.749986
PRIMER_LEFT_1_SEQUENCE=GAAGGTGGAGGCCCTTTAATAC
PRIMER_RIGHT_1_SEQUENCE=AAGCAAGCACTGTGTTAGAGTCA
PRIMER_LEFT_1=1449,22
PRIMER_RIGHT_1=1657,23
PRIMER_LEFT_1_TM=60.199
PRIMER_RIGHT_1_TM=59.250
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=1.9644
PRIMER_LEFT_2_PENALTY=0.214429
PRIMER_RIGHT_2_PENALTY=1.749986
PRIMER_LEFT_2_SEQUENCE=AGGCCCTTTAATACCTTGCATT
PRIMER_RIGHT_2_SEQUENCE=AAGCAAGCACTGTGTTAGAGTCA
PRIMER_LEFT_2=1457,22
PRIMER_RIGHT_2=1657,23
PRIMER_LEFT_2_TM=60.214
PRIMER_RIGHT_2_TM=59.250
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=2.0073
PRIMER_LEFT_3_PENALTY=0.257312
PRIMER_RIGHT_3_PENALTY=1.749986
PRIMER_LEFT_3_SEQUENCE=CCCTTTAATACCTTGCATTTGG
PRIMER_RIGHT_3_SEQUENCE=AAGCAAGCACTGTGTTAGAGTCA
PRIMER_LEFT_3=1460,22
PRIMER_RIGHT_3=1657,23
PRIMER_LEFT_3_TM=59.743
PRIMER_RIGHT_3_TM=59.250
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=2.0643
PRIMER_LEFT_4_PENALTY=0.314269
PRIMER_RIGHT_4_PENALTY=1.749986
PRIMER_LEFT_4_SEQUENCE=AGAAGGTGGAGGCCCTTTAATA
PRIMER_RIGHT_4_SEQUENCE=AAGCAAGCACTGTGTTAGAGTCA
PRIMER_LEFT_4=1448,22
PRIMER_RIGHT_4=1657,23
PRIMER_LEFT_4_TM=60.314
PRIMER_RIGHT_4_TM=59.250
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_04938 Ps_contig_mira-and-tgicl-ass_franssen_35371
SEQUENCE=TCAACGCAGAGTACGCGGGGGAGGAGGAGGATGAAGCGGCGTATGAATCCGGCGAGAAAATCGTGGTGGTGGATTTCGAATTTGATTCCGTGGATGATTCAGCGGTTCCTCCTTTTTCATGGAAGAAGCTATGGATGTTCACTGGACCTGGATTCTTGATGAGTATAGCTTTTTTGGTATCCGGGGAATCTGGAAGGGGATCTGCAGGCCGGAGCGATTGCCGGATATTCGCTGTTGTGGTTGTTGATGTGGGCTACTTTCATGGGGTTGTTGATTCAGCTTCTTTCTGCGAGAGTTGGTGTTGCTACTGGTCGTCATCTTGCTGAGCTTTGTAGGGAGGAGTACCCTAATTGGGCTCGTTACGTGCTTTGGTTTATGGCGGAATTGGCTTTGATTGGGGCTGATATTCAAGAGGTTATTGGGAGTGCGATTGCTATTCAGATTCTTAGCCGTGGTGTTTTGCCTCTTTGGGCTGGTGTTCTTATCACTGCTTCTGATTGCTTTTTCTTTCTATTTCTTGAGAACTACGGAGTAAGGAAGTTGGAAGCTGT
TARGET=20,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04939 Ps_contig_mira-and-tgicl-ass_franssen_35381
SEQUENCE=CGTTACTATACACGAAGAGANAACCAAGCTTCAGCAAATTTAAGTAATGCAAAATTAAACTTACGCTAAAATTAATCAACCTTCCAGAAAAACGGGACCCCTCCTTATCAAACAGCTACCTTCGGTATTCAATTCTAACAAGCCAAGTAGAACTTGTTGAATTTCTAGCCGATCCAATATAGGATAAATGTTAATCAGTGGATCAACCACACAGAAATCCTGATTCTGATCCAAATACCTTTGCAGTTCTTGCATGTGTTGTGTAAACGTAAGTTTGTTCTCTTCAATGGATAAAATTTCATCAGTGGCCTTATGAAGGACAATGTCAACATCTTTTAACTGAGACGCTAAAGGAAGCGTGGCCATAAGAGGCACAAACATTAAACCATTTTCTGTAGGACACAACGGGAATGCACCCCTCTTCGCAAAATCTTCAACACGAGAAGGCTTCATCGTATAACCCACAGTCAAGAAATTGTTTCCGGCTAACCTCTTATTTAACCGACAAATCGTCAATGGCAACTCCGCTAAATTCTCAATATTTGGAGAGTCGGATTCACATGAACTTTCACTTTCACTTTCACTTTCCCCTCCAACATTCAGAACAACAACCAACCAATTATAACTTCTTAATTTAGGTAATAACAACTTCTTCTTACTAGAAACCAGATAAATAACACTCTCATTCTCAATATCACACCATTCAGGCATAACAACCTCATTTACAGGATCATTTAAGACAAACCAATGAAGTGAGTATAAACTAGCTATTCTTTTCAGAATACTAACCTTATCGTTCGGAAGATCAACACTATAAGCAATTCCAACATGCGTTCCGGACAAAAAGAGTGTTTTGATGAGAGATTCAACTCCTGGCTGTAGTGATGATACATTTTTATCATCATCACCATCTCCTCCAACCAAGAGTACCGATTCATCCAATATGACACCTTTCACTACACCCATCTCTGAGAGTGGATGCAACACTTAAAGATTTTGTTACTGCTGTTACAGGACGTTGAGGATGRTCTCAGACTGGTACTTTTCGGGAAGTAGAGTTAGTTAGTTCATTGTTACAATCCGGNATTGTTTACAAAACACAAGGACAAGCGGATGCCAATTCAATACAAAATATCTA
TARGET=1057,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04940 Ps_contig_mira-and-tgicl-ass_franssen_35436
SEQUENCE=TAAAATGAAGCATGTTTGATTTTTTCTTCACTTATTTCTTTGTTTATTATTATTACAGGTCATGTCAAATCAAGAAGCAGTTGATGCTATCAAAGATATCAAGGATGCTCGATCTGCAGCAAAGCACCTTACTGAAGAAGCGCTTAAACAAGGAAAANCTACAGATGATATCTCTTGCTATTGTTGTGTAGTTCATGATATA
TARGET=44,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04941 Ps_contig_mira-and-tgicl-ass_franssen_35443
SEQUENCE=TTAAAATATGTAAGAACATGTTAATAATTCTACCTTTGAATAGTTGGTAGATTCTTAGATGAATATATCAATATTGAATTAAAAGTTCACATACAAACAAACATAAATACTAAAATAAACATAACATGAACAACAACAATAATTAACAACTCAATAGTGAAAAACATAAACATTGTACTCAACACAAGCATCTCCAGATTTGTAATCAATCCAATGAGTATTAGCTTGAGCATAGCCTCTGGCGAATCTGAAGAGTCCACTCCCACCAACAACAGGCATCTCTCTCACCTTATTCATAGCAGGATTCTTCCCCAAGATAGTAATAGTACTTCCATTATACTTTCCTTCAATGAAAGCAAAATTCATAGCCATAAGTAAACCAAGTTCAGCCTGTGAAGCAGATGCGTAGAAGCCTTGAGCTTTTCCAACAAGTTTGGAACTCAATTCTGGTCCTAATGTTAAAGGGTTGTCGATCATGTTGACTGAACCRAAATAAGTGGTTGAGTTTAATGATGGTGGAAGAATTGAAACTGAAGATGGGTTTTTTCCACTYAGAATGTCRTGCCAGTAGAATTTGAAATGACTTAGTTTTTCCTTCTTGGTTAGGCCTAACAGCTTGCGATCTATAGGACGAACAAAATCGAAAGTGTCTTTATTTTGTGATGATGTTGAAGAAGGGATGGTGTTGCAAGAGAGGAAAAGGAAGAAGAAGAAAGTGAGAAAATGTTGGGAAGACATTGTGATGAGGTTTGTGAGATTGTTAT
TARGET=703,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04942 Ps_contig_mira-and-tgicl-ass_franssen_35444
SEQUENCE=CTTGAGGAAGTGAAAACCACGCGCCTCACATTTGCTTCTGCAGCAGCTATGATTACATTCTTTGCTCCATTTTTACTGCAGGCTCCACCATCTCTTCGGGGTTATCGGTAACGGGAGAAGCAGTATGAAAAACACCGTGACAACCGTGAACGGCAGCTTTAACGGAGTTAAGATCAAGTAGATCAACTTTATGCAGAGTTAGTCTCTCCTTCGCTCCTTCCAACTCTTTCAAGTGTCCATTTTTTGCATCATCTGGGTTTCGCAAGGTTCCTCTGACGGTGTAGCCTTTTTCAAGGAGGAGTTTAACCATCCATGAAGCGATGAATCCGCCGGCGCCGGTGACGCATATGGTTTGGCCGGAGATTGGTGAAGGTTCTGCAGCGGCGGCGGCGGAGGCGGCAGGCATATTCGGGATTGGAAAAGGACAAAGGAAGGTGAATGAGTGAGTGGAATAGAGAAGTGATGGGAAAGTTGTGACTTGTGACGTGGGGTTGTAATAAGTGGAAATATCCCCCC
TARGET=381,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1374
PRIMER_LEFT_PENALTY=0.113803
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=TGTAGCCTTTTTCAAGGAGGAG
PRIMER_RIGHT_SEQUENCE=TCATTCACCTTCCTTTGTCCTT
PRIMER_LEFT=279,22
PRIMER_RIGHT=441,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.1646
PRIMER_LEFT_1_PENALTY=0.113803
PRIMER_RIGHT_1_PENALTY=0.050763
PRIMER_LEFT_1_SEQUENCE=TGTAGCCTTTTTCAAGGAGGAG
PRIMER_RIGHT_1_SEQUENCE=GTCACAAGTCACAACTTTCCCA
PRIMER_LEFT_1=279,22
PRIMER_RIGHT_1=484,22
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=60.051
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=0.1823
PRIMER_LEFT_2_PENALTY=0.158697
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=GGAGTTTAACCATCCATGAAGC
PRIMER_RIGHT_2_SEQUENCE=TCATTCACCTTCCTTTGTCCTT
PRIMER_LEFT_2=297,22
PRIMER_RIGHT_2=441,22
PRIMER_LEFT_2_TM=59.841
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=0.2095
PRIMER_LEFT_3_PENALTY=0.158697
PRIMER_RIGHT_3_PENALTY=0.050763
PRIMER_LEFT_3_SEQUENCE=GGAGTTTAACCATCCATGAAGC
PRIMER_RIGHT_3_SEQUENCE=GTCACAAGTCACAACTTTCCCA
PRIMER_LEFT_3=297,22
PRIMER_RIGHT_3=484,22
PRIMER_LEFT_3_TM=59.841
PRIMER_RIGHT_3_TM=60.051
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=0.2105
PRIMER_LEFT_4_PENALTY=0.186949
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=GGAGGAGTTTAACCATCCATGA
PRIMER_RIGHT_4_SEQUENCE=TCATTCACCTTCCTTTGTCCTT
PRIMER_LEFT_4=294,22
PRIMER_RIGHT_4=441,22
PRIMER_LEFT_4_TM=60.187
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_04943 Ps_contig_mira-and-tgicl-ass_franssen_35467
SEQUENCE=TTCACTGTTAAAGGAGGAGAAAAAGTGAACATACAAATAGAAGGAGTCGAGAGTGGTGCAACAATCAAGTGGGAGATTGTAGTTGGAGGCTGGGAAGTACAATACAGTGCTGAATTTGTCCCAAATGCAGAAAGGAGCTACACAATAGCAGTTGATAAGGCAAGGAAAGTTGATGCTTCAGAGGAAGTAATTGAGAGTTCATTTACATCAAAAGAAGGAGGCATAATAGTACTCACAGTTGATAACTCTGGTTCAAGGAAGAAAAAAGTTGGTGCTTATCGCTATTTTGTCTCCAAATGCAACAACAATAATACAGCTTCTGATAATATGCAATTAATTTTGTATTAGTAAAAAAGTGAGTAAAGAAGAAAAAGCAATTGGCTTTGGTTTGGCGTATTGAGATGAGTTTTGTCAGAGGGTGTTGTCGTTATTATTGGCATATATGTGTGTGTGTGTGAAAGTACGAGTGAAATG
TARGET=444,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04944 Ps_contig_mira-and-tgicl-ass_franssen_35486
SEQUENCE=GAAATCGAAGAAAGAGATGAAGAGTTGTATATCAGGACTAATAAAAATAACAATACTTGGAGCAACAAATAATTCTAAACAAGGGATACAATTGCGGTATCTCAACTTCTAGAGGATTTTATCTGTACCATTTTTAACTACAATTCTGTTTTCAAATTCCCATCCTCTTGACCAGCATTATCTGCTTCGCCTTTCTCTCTATGTTGCTTCTGAATGAGTTCGTCAAGGGGTAGATCTCCAGGTCTTCTAAAAAATTGCTCCCCCATTTTAATCTCATATGCTTCTGATTGGTCCAAAACAAATTCCTTCAACTCCTCTAAGTCTTTGATGCTGTCCATGTTGAACATGATTGTATTCAGATCAACACCTGTAAACCTTATTCCAATAGCTCCAGTTCTCAGAACACGTGTCCATTTCATGGCAAGTTCAGCTACCATGTCCGGAGTCCGGCGAACACCTAATCGGAGCTTGACGAATCCGATAACAGGTCCGGTGTGGCGTTTCATCATCTCCTCTTGGATCTGCGAAGTATCCATCTTGGTTATATCGGAGGGAGCGAAAGAGGGAGCAGGTTTCTTGCCCCATTGTTTCCAATCATCATCTTCCTCATCGTCGAACACGTCGTCCAATTCGTCGGTGATGTGGACCTTGCGTTTGCCGGCGAGGGCAAAATGGGAATTTAGCGAGATGAGAAGAAGAAAAAGGAGAAAGAGAGTGGTGAGGTTCATGATGAAATGCAGTGTGCAAAGAAGAATGAATGAATCCGATAGAAGAAAGAGAGAAAGAAGTGTTGATAATGTGTTCACTTCATACAATCATGGCTTTGGTGGTTCGTCTTTGCATACCCC
TARGET=752,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5524
PRIMER_LEFT_PENALTY=0.061230
PRIMER_RIGHT_PENALTY=0.491155
PRIMER_LEFT_SEQUENCE=ATGGGAATTTAGCGAGATGAGA
PRIMER_RIGHT_SEQUENCE=ACCACCAAAGCCATGATTGTAT
PRIMER_LEFT=671,22
PRIMER_RIGHT=830,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=60.491
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=0.5873
PRIMER_LEFT_1_PENALTY=0.061230
PRIMER_RIGHT_1_PENALTY=0.526059
PRIMER_LEFT_1_SEQUENCE=ATGGGAATTTAGCGAGATGAGA
PRIMER_RIGHT_1_SEQUENCE=CCAAAGCCATGATTGTATGAAG
PRIMER_LEFT_1=671,22
PRIMER_RIGHT_1=826,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=59.474
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.6548
PRIMER_LEFT_2_PENALTY=0.163604
PRIMER_RIGHT_2_PENALTY=0.491155
PRIMER_LEFT_2_SEQUENCE=CATCTTGGTTATATCGGAGGGA
PRIMER_RIGHT_2_SEQUENCE=ACCACCAAAGCCATGATTGTAT
PRIMER_LEFT_2=533,22
PRIMER_RIGHT_2=830,22
PRIMER_LEFT_2_TM=60.164
PRIMER_RIGHT_2_TM=60.491
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=0.6548
PRIMER_LEFT_3_PENALTY=0.163604
PRIMER_RIGHT_3_PENALTY=0.491155
PRIMER_LEFT_3_SEQUENCE=TCCATCTTGGTTATATCGGAGG
PRIMER_RIGHT_3_SEQUENCE=ACCACCAAAGCCATGATTGTAT
PRIMER_LEFT_3=531,22
PRIMER_RIGHT_3=830,22
PRIMER_LEFT_3_TM=60.164
PRIMER_RIGHT_3_TM=60.491
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.6897
PRIMER_LEFT_4_PENALTY=0.163604
PRIMER_RIGHT_4_PENALTY=0.526059
PRIMER_LEFT_4_SEQUENCE=CATCTTGGTTATATCGGAGGGA
PRIMER_RIGHT_4_SEQUENCE=CCAAAGCCATGATTGTATGAAG
PRIMER_LEFT_4=533,22
PRIMER_RIGHT_4=826,22
PRIMER_LEFT_4_TM=60.164
PRIMER_RIGHT_4_TM=59.474
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_04945 Ps_contig_mira-and-tgicl-ass_franssen_35488
SEQUENCE=AGTTTCAACCTAAACTTATTACCTTTTACACAAAGATTAAAGGGTACAACAGAACAAGAGTTTGAAACATGTCAGATAGAATTCCAACACAAAAATAATCAATGAAAAAATGACGTACTAGAGGTTTCGGTTTTAACTGCTCCTAGAAATAACAAAAACAAAAACAAAAACTACATTTTTCAAATAAACTTCCCTAGTGGCAGATATTGAACATTCTTTAGTATCTGTTGTAGGAGAGCTACAATCACAATTTTTCATCTTCACTTGCAAGCCAAAACACAATTTTTGAAACATTGACTGCACCTCCTCCTCTTTCAGAAGAAGATACAGAAATAGGAGAAAGAATATTAGCGTCGAAAAGGAGGTGAAACCGCGAAACAAAAAGGAAAAAAAATTGTTCTTTTTCTCCTACCCCAGAATTGGGAAACAGCCACTTCTGGATCTTGTCTAAAAGCTCTGAAGGGCACCTGTAGTGTGAGTGAGAGTTAACTTAGTAGCCTCCAACTTAGTGGTTGGTAGGAACGGAAAAGAAGCCTTCTCCACAAGCAAAACGCGCATGTATGTAATCTTCAATCTTCTCTCTCTCTCTCTTTATGCATAGTACATGTACAAGCTATTTGCTGCAGCACAAGCAATTGAGTTTTCAGATGGATGCCATGCTAAGTGCAACAGCTTTGTCGTGAAATCAAACGAATTCCCGTTTGCATCAACCCCGGGGTTTTCTGCTCCCCGTCTTACAACTCGTGTTAGACTATTTCCGAGTGATCTAGACGGCCTTGAAGGGGTCGGAACTTGTCGTCTCATTGGATTTTTACTAGCTTCCAAAGTTGTAGCCTCGGTACTTCCAGGAGAACAGCCAAATACTCGGAATAAATTACTGTAAGAACCAGTTGCCACCCGCAGTCCATCACCGCTCAGACAGCACTCGAACTTATACGAAAATCGAATTCGTTTTCATATAAGTCACAAAGCTTAGGTCTTAGATA
TARGET=577,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1001
PRIMER_LEFT_PENALTY=0.026163
PRIMER_RIGHT_PENALTY=0.073900
PRIMER_LEFT_SEQUENCE=TTGTTCTTTTTCTCCTACCCCA
PRIMER_RIGHT_SEQUENCE=GAAAACTCAATTGCTTGTGCTG
PRIMER_LEFT=394,22
PRIMER_RIGHT=644,22
PRIMER_LEFT_TM=59.974
PRIMER_RIGHT_TM=59.926
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.2354
PRIMER_LEFT_1_PENALTY=0.026163
PRIMER_RIGHT_1_PENALTY=0.209271
PRIMER_LEFT_1_SEQUENCE=TTGTTCTTTTTCTCCTACCCCA
PRIMER_RIGHT_1_SEQUENCE=GTTTGATTTCACGACAAAGCTG
PRIMER_LEFT_1=394,22
PRIMER_RIGHT_1=690,22
PRIMER_LEFT_1_TM=59.974
PRIMER_RIGHT_1_TM=59.791
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.2484
PRIMER_LEFT_2_PENALTY=0.174518
PRIMER_RIGHT_2_PENALTY=0.073900
PRIMER_LEFT_2_SEQUENCE=AACAGCCACTTCTGGATCTTGT
PRIMER_RIGHT_2_SEQUENCE=GAAAACTCAATTGCTTGTGCTG
PRIMER_LEFT_2=425,22
PRIMER_RIGHT_2=644,22
PRIMER_LEFT_2_TM=60.175
PRIMER_RIGHT_2_TM=59.926
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.3536
PRIMER_LEFT_3_PENALTY=0.026163
PRIMER_RIGHT_3_PENALTY=0.327468
PRIMER_LEFT_3_SEQUENCE=TTGTTCTTTTTCTCCTACCCCA
PRIMER_RIGHT_3_SEQUENCE=GGCATCCATCTGAAAACTCAAT
PRIMER_LEFT_3=394,22
PRIMER_RIGHT_3=655,22
PRIMER_LEFT_3_TM=59.974
PRIMER_RIGHT_3_TM=60.327
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.3838
PRIMER_LEFT_4_PENALTY=0.174518
PRIMER_RIGHT_4_PENALTY=0.209271
PRIMER_LEFT_4_SEQUENCE=AACAGCCACTTCTGGATCTTGT
PRIMER_RIGHT_4_SEQUENCE=GTTTGATTTCACGACAAAGCTG
PRIMER_LEFT_4=425,22
PRIMER_RIGHT_4=690,22
PRIMER_LEFT_4_TM=60.175
PRIMER_RIGHT_4_TM=59.791
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_04946 Ps_contig_mira-and-tgicl-ass_franssen_35504
SEQUENCE=AAGTCAATCAGTCATTGCACATTAATTATACTAAAATCTGAAGTTCCCACTCATTTTTCCATGTGAAAAAGCTTGTAGAAAAATTATAGAAGATAACATAACATCCTCGTAATCAAAATTCTCAAATGATTTTTTTATGAACCAATGCAGGAAAGAATATACACAATTAACTAAAACAAATAACCTCCTCATAACCAATAGCTTCGATGGAAGCTGTAATTAGGAACTGAGGCCTAACTGAAGTCCAAAGGCAGCATGAATAAGAAAGAGTGTCATGATACTACCACCCAAGATGCCATGAACATTCCTTAATCCAGGATTTCCCTCAAATAGTGAAGGTAATATAGTCTGTATTGTCAACAAAGCAAGGCCAATAACGCCTGTAACAGCATGAGGACTGTCAAAAATGGGTTTATCTGATGTGAGTAAAGATGTTACTCCACCGGTAGCTCCCAGAGCAAAAAAGAAAAACATTCCAGCTAAAAGCTTGGGATGCAAGTCTTTGGCCTTAGCCTTTTCCTCCGCGTCATCGGAAAAACGTATACGGAAGCCGAGATAAGTACCATAACCACCCATAGCAAAGAGAACGACTGCCATATTGGCAGGGTGACCCCAATGTACAAGCCAATCCGGTAGATTCAACGACTTCACTATGTCAACAAAAGGCTCAAACGCAGATTTCACAGCTCCATCTCCGGGGATGAGAGCGGCGGTGTAAAGTAATGGCAACGGAGAGAAAGAATGTAAGATCGTTTCAGTGTTTGTTCTCGTCGATTGTAGAGGTTCCGGTGAGTCCTCCTTTGTGGCGAGACATTTGAGATTGTGACGAAGGTGCAAAGGTGAAGTTGTTGTTGTTGTGGGGAAAGAAGAGTGGCGAATTGGTGATAGAGGACGGAGATTCACGCCTATGGAAGAAGAAGTCACGATTTCCATTTGGTATGGGTAAACAACACCAACAGAAAATAGAGAAGTTGTTGATGTTGTTATGATGGAAAATGAGAAGTTGTTGTTGTAATAGTGAAAATGTAAGATGGAGAGTGAGTGGGAGTCCCCGAC
TARGET=845,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1675
PRIMER_LEFT_PENALTY=0.129103
PRIMER_RIGHT_PENALTY=0.038361
PRIMER_LEFT_SEQUENCE=AAGTAATGGCAACGGAGAGAAA
PRIMER_RIGHT_SEQUENCE=AATCTCCGTCCTCTATCACCAA
PRIMER_LEFT=717,22
PRIMER_RIGHT=899,22
PRIMER_LEFT_TM=60.129
PRIMER_RIGHT_TM=59.962
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.1675
PRIMER_LEFT_1_PENALTY=0.129103
PRIMER_RIGHT_1_PENALTY=0.038361
PRIMER_LEFT_1_SEQUENCE=AAAGTAATGGCAACGGAGAGAA
PRIMER_RIGHT_1_SEQUENCE=AATCTCCGTCCTCTATCACCAA
PRIMER_LEFT_1=716,22
PRIMER_RIGHT_1=899,22
PRIMER_LEFT_1_TM=60.129
PRIMER_RIGHT_1_TM=59.962
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.1675
PRIMER_LEFT_2_PENALTY=0.129103
PRIMER_RIGHT_2_PENALTY=0.038361
PRIMER_LEFT_2_SEQUENCE=AAGTAATGGCAACGGAGAGAAA
PRIMER_RIGHT_2_SEQUENCE=CTCCGTCCTCTATCACCAATTC
PRIMER_LEFT_2=717,22
PRIMER_RIGHT_2=896,22
PRIMER_LEFT_2_TM=60.129
PRIMER_RIGHT_2_TM=59.962
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.1675
PRIMER_LEFT_3_PENALTY=0.129103
PRIMER_RIGHT_3_PENALTY=0.038361
PRIMER_LEFT_3_SEQUENCE=AAAGTAATGGCAACGGAGAGAA
PRIMER_RIGHT_3_SEQUENCE=CTCCGTCCTCTATCACCAATTC
PRIMER_LEFT_3=716,22
PRIMER_RIGHT_3=896,22
PRIMER_LEFT_3_TM=60.129
PRIMER_RIGHT_3_TM=59.962
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.2191
PRIMER_LEFT_4_PENALTY=0.180765
PRIMER_RIGHT_4_PENALTY=0.038361
PRIMER_LEFT_4_SEQUENCE=CGTTTCAGTGTTTGTTCTCGTC
PRIMER_RIGHT_4_SEQUENCE=AATCTCCGTCCTCTATCACCAA
PRIMER_LEFT_4=750,22
PRIMER_RIGHT_4=899,22
PRIMER_LEFT_4_TM=59.819
PRIMER_RIGHT_4_TM=59.962
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_04947 Ps_contig_mira-and-tgicl-ass_franssen_35505
SEQUENCE=ACCCACATCAAGAATAGCGTAAAAATTACAATCTTTTTTCTAACCCCGTCAAAAAATAGTCGAACAAGATAGTTCTCCGGCAAGCGGCGTCGGCACATCGCCGACAACCGTTGCTCCAGAGCCAATCAACGAAAACAGCCAACATGGTCGGCGAAGTAGTCGGAAGCACCGCGGCGGAGTGCTTGGCCGTATGCTGCTGTTTTCCATGCAGTTTAGCGAATATTTTTCTGTTAGCAGTTTACAAACTCCCGGCGGGTATTTGCCGGAGAATTCTAAGAAAGAGACGACGGCGGAGAATTATGAAAGAAGGGTTGTTACAACCGAAACGCCTTAACTGTTCTTGCGGTGGTTGCGACGACGTTAACGGTGTACCTCGTATTTACCCCATGTGTATAAACGACGCTTCAGATATAAAACGACTTCATGATGGTTCGTTTCAAAACAACAATGATGAAAATGCTATGGCTCTTGAGAAAGAGATGTGGGAGAGATTTTACAGTGCTGGATTTTGGAGAAGTTCTTCGAGGAGAGAAACAGAATCGGCACCAAGAACAGAAATTGTTTCTGGAAAATTTCAAGTTCAATTGATTGAATTGAAACNAACATCGGTTGATGAATGAGGAGACAAAAGAGTCAAGTCAACAGTGGTTTTTGAATTGATTGGTTATTTGTTTGTACTATTTGGAAATGAAACTTTTTGTGGTTTTTTAATTACTATTCTTACTGTTGAGGACTGTGCATTTGTGACTTGTTTTTTTTATTTGGGTCCCGTGTCTCTTTTCAATGCGCTATGRTCCTCGATTGGTGTGTGTGTGTGGTGGTACAAGAGAGTAATTTTTTAAATTTTTTATTGTTTGTAATTTATTGAATTGTAACGTTAAAGACAAAACGTAAAGTTTTATTTATAAT
TARGET=805,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04948 Ps_contig_mira-and-tgicl-ass_franssen_35519
SEQUENCE=AAGCAAGGATGACAGATATGCGGTTGATTCTGTTGTGACGCATGATGGGATCAAATTTCCTTGCTGGGCTTTGCCAGATTTGATGTTGTTTAAAGATAAATTTGGTCATGAGGCTTATGAGAAGTTGGATGTGATTGGTATAGATGAAGCTCAGTTTTTTGAGGATCTATATGATTTTTGCTGCAAGGCTGCTGATGAAGATGGTAAAATTGTTGTTGTTGCAGGCCTAGATGGTGATTACTTGAGGTATTATTTGGAGAAAAATCCTTAAAGGCTTTTACTGTTTATTTTAACTC
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9428
PRIMER_LEFT_PENALTY=0.110942
PRIMER_RIGHT_PENALTY=1.831903
PRIMER_LEFT_SEQUENCE=ATATGCGGTTGATTCTGTTGTG
PRIMER_RIGHT_SEQUENCE=CCTCAAGTAATCACCATCTAGGC
PRIMER_LEFT=15,22
PRIMER_RIGHT=246,23
PRIMER_LEFT_TM=59.889
PRIMER_RIGHT_TM=59.168
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=2.1843
PRIMER_LEFT_1_PENALTY=0.110942
PRIMER_RIGHT_1_PENALTY=2.073361
PRIMER_LEFT_1_SEQUENCE=ATATGCGGTTGATTCTGTTGTG
PRIMER_RIGHT_1_SEQUENCE=ACCTCAAGTAATCACCATCTAGGC
PRIMER_LEFT_1=15,22
PRIMER_RIGHT_1=247,24
PRIMER_LEFT_1_TM=59.889
PRIMER_RIGHT_1_TM=59.927
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.833
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=2.2348
PRIMER_LEFT_2_PENALTY=0.402858
PRIMER_RIGHT_2_PENALTY=1.831903
PRIMER_LEFT_2_SEQUENCE=TGACAGATATGCGGTTGATTCT
PRIMER_RIGHT_2_SEQUENCE=CCTCAAGTAATCACCATCTAGGC
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=246,23
PRIMER_LEFT_2_TM=59.597
PRIMER_RIGHT_2_TM=59.168
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=2.2348
PRIMER_LEFT_3_PENALTY=0.402858
PRIMER_RIGHT_3_PENALTY=1.831903
PRIMER_LEFT_3_SEQUENCE=GACAGATATGCGGTTGATTCTG
PRIMER_RIGHT_3_SEQUENCE=CCTCAAGTAATCACCATCTAGGC
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=246,23
PRIMER_LEFT_3_TM=59.597
PRIMER_RIGHT_3_TM=59.168
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=2.4762
PRIMER_LEFT_4_PENALTY=0.402858
PRIMER_RIGHT_4_PENALTY=2.073361
PRIMER_LEFT_4_SEQUENCE=TGACAGATATGCGGTTGATTCT
PRIMER_RIGHT_4_SEQUENCE=ACCTCAAGTAATCACCATCTAGGC
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=247,24
PRIMER_LEFT_4_TM=59.597
PRIMER_RIGHT_4_TM=59.927
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.833
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_04949 Ps_contig_mira-and-tgicl-ass_franssen_35522
SEQUENCE=TAACGCTCTTCAAACTCCAAGCAACACCATGTCTCCCAATTATGTAGCCGAGAGACTTGAGATGTCTGTAAGCCTCAAACAGCTCCCAGCAACATCCACTCTTCCCGCCTGCAACCTTATCGTACATATCTATTAGAGATATACTTCTATTTTCATCGTCTACGGGATGTAAGGCTCCTACTTCCATAAGATACAAAGTCTCCTCGATTGAAGTATAAATCTTTCCACTGCGAACTATTCCAGTTGATACCCACATTTTCCCATTCTTCTCAATAACCTCTGCCATTCCCATTTCTTCATTCCACCTACCTTTTGATTTCACGTTCCTGAATTGCAATTTGGGCAAGGAACTAGATGTGAAGTGAAGGTTCTCATCATCGCTTGCAATTTTGCAAAAAGACTTGATCATTATCATCATCATCACTNT
TARGET=411,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04950 Ps_contig_mira-and-tgicl-ass_franssen_35525
SEQUENCE=AGTATAATAAGTCAATTTATAATCTTTAAACACCAGCTTTGAACCCAACCTTGCTTTCGTTTCTNTTTGTGGTGACATAAATCTCCCCCTACAACGCATGAATTAAGAATTATAGCAACAACAACAAGGTCTACTCGACATAACTTAGGAGTTAATAAAACCTTTTTACAAANGATCCATCAACTAAATTATTATCAATGAATCCGAA
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04951 Ps_contig_mira-and-tgicl-ass_franssen_35547
SEQUENCE=TTTTTACTTAAAATAAGAAAATAAAGGAAAAGTTTTTAATTTTTTAGTTAGTGCAATAGCGATGCTCTACTACTACTAAGAAAAAATAAATNGATCAGGCTAACAAAAACATGAAATCTAAATAAGAAGAAGCTTATTTATTTTTCTCCATTTGTCTTCCACCATGTCTCAAACTTTGAGAGAAACATTGAGAGGGAAGTTTAATCCTTGTACTTCATCTCAGATACCAGGAAACCAGGCATACCAAATGAGGAAGAGAACTTCTCAACATCAGCTTTGAGTTCTTCAATAGCTTTGTTGTCAACTAAGCCCTTGTTGAAATCCTTCAAAAGTTTGCCATGCTCCTTTTGGATCTCTAGTGTGAGAGTCACAGCACGGTGAAGGAACTCTCCAATCTGCTCAAAGTCCTTTTCAACCAAACCTCTAGATGTCATTGCCGGAGCACCGACTCGTACTCCTCCAGGAGCCAAGGCACTGCTATCGCCAAAAACAGCATTTTTGTTCACTGTGATGTTACAGAGGTCACAGAGTTTCTCCACCTTGTTTCCGGTCAATCCAAGAGGGCGGAGATCCCACAATACAAGATGGTTCTCAGTTCCACCAGTGACAAGACTGTATCCCTTGCTCATCAAGTAATTTCCAATGGCAACTGCATTGGCCTTAACTTGCTTTGCATATACTTTGAACCCAGGTGACATGGCCTGTTTCAAGGCAACAGCAAGGGCACCAATCTGGTGATTGTGAGGACCACCTTGGAAGGGGAGGGGAAGACTGCAAAGTTGATCTTGTCCT
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04952 Ps_contig_mira-and-tgicl-ass_franssen_35548
SEQUENCE=TCGGGGTATCATGATGACCTCTACAGGGCCATCGACATTTATCTCAAGGCTCACCCAGAACTCAACAAGAGTGAAAGGAAAAGATTATGCAGAATTCTCGACTGACAAAAAAACTGTCAAATGGAAGCATGCATGCATGCAGCAACAAAATGTACGTTACCTTACCCTTAAG
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.8154
PRIMER_LEFT_PENALTY=0.154878
PRIMER_RIGHT_PENALTY=2.660570
PRIMER_LEFT_SEQUENCE=TCGACATTTATCTCAAGGCTCA
PRIMER_RIGHT_SEQUENCE=GGTAACGTACATTTTGTTGCTG
PRIMER_LEFT=31,22
PRIMER_RIGHT=160,22
PRIMER_LEFT_TM=59.845
PRIMER_RIGHT_TM=57.339
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=2.8846
PRIMER_LEFT_1_PENALTY=0.224014
PRIMER_RIGHT_1_PENALTY=2.660570
PRIMER_LEFT_1_SEQUENCE=CTACAGGGCCATCGACATTTAT
PRIMER_RIGHT_1_SEQUENCE=GGTAACGTACATTTTGTTGCTG
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=160,22
PRIMER_LEFT_1_TM=60.224
PRIMER_RIGHT_1_TM=57.339
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=3.0457
PRIMER_LEFT_2_PENALTY=0.385098
PRIMER_RIGHT_2_PENALTY=2.660570
PRIMER_LEFT_2_SEQUENCE=ATTTATCTCAAGGCTCACCCAG
PRIMER_RIGHT_2_SEQUENCE=GGTAACGTACATTTTGTTGCTG
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=160,22
PRIMER_LEFT_2_TM=59.615
PRIMER_RIGHT_2_TM=57.339
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=3.1681
PRIMER_LEFT_3_PENALTY=0.154878
PRIMER_RIGHT_3_PENALTY=3.013232
PRIMER_LEFT_3_SEQUENCE=TCGACATTTATCTCAAGGCTCA
PRIMER_RIGHT_3_SEQUENCE=GGGTAAGGTAACGTACATTTTGTTG
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=166,25
PRIMER_LEFT_3_TM=59.845
PRIMER_RIGHT_3_TM=59.987
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=3.1738
PRIMER_LEFT_4_PENALTY=0.513182
PRIMER_RIGHT_4_PENALTY=2.660570
PRIMER_LEFT_4_SEQUENCE=ACATTTATCTCAAGGCTCACCC
PRIMER_RIGHT_4_SEQUENCE=GGTAACGTACATTTTGTTGCTG
PRIMER_LEFT_4=34,22
PRIMER_RIGHT_4=160,22
PRIMER_LEFT_4_TM=59.487
PRIMER_RIGHT_4_TM=57.339
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=127
=
PRIMER_SEQUENCE_ID=SSR_04953 Ps_contig_mira-and-tgicl-ass_franssen_35564
SEQUENCE=TTTCGGCGATCACCACCACCACAACCACGACACCGATTTTCTTCGAAGCTTCCCTCCTTCTCTCAGAAACAATTACACACTATGGTATGTGGAGACACCGTTTTCAAGATGAGTTACAATTGAAATCACCCAAGTTATCATTTCCACAACATAGTTCAAGAATGGATAATAGTTTGTTCAGACAAGATGTTCTACAGCGTTTTCAGAATCCCACAATTTTTCNCAAGATTTCTTGTGATGGAGACTATGTTCTGCCCATTGCTGTAAGGAAAGNTTGCTATTAGAAAAGCTTAATAGATGCTAGAAGTTGAAAC
TARGET=11,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04954 Ps_contig_mira-and-tgicl-ass_franssen_35582
SEQUENCE=ACGCGGGGAAATTGAGAGATTCATATCTGCAATTTTTAACTCTCCAGTCGTCTTCTAATTCTTCACTCTCTCTGTAACTCGCTTCTCTCACTTACCAAACCCTAATTCCCAATTTTACCACTCTCGCCGCAATCCCTCACCATCAGATTCAAAAATCCACACTTTATACCAATTACTTCGTTTCCCGTTATTGATTTTCTCTTCTATATATTTAATTTGATTTGATTTGATTTGATTCTTCTCACTCTAATCTCACGATGAAAGAAGGTAAACTAAATCATCAACTTCAGCACCAGAACGGTCACCTTTCTCCTTTCAAATTCGCCAAATTGCTCGATCCCGAAGCTTCCTGGGACAAGGATCAACTTGGAGATGTATTGCATTGGATTCGACAAGCAATGGGCCTTGTATTTGGACTACTTTGGGGTGCCTTTCCAGTGGTTGGGGGATTTTGGTTTATCCTCTTTTTAGTTTTGTCCTCCGGGATCATATATGGTTACTATTCAGTAATATTGAAGGTTGATGAAGAGGAATTTGGCGGCCACGGATCCCTTCTCCAAGAGGGGCTTTTTGCTTCTACAACTCTATTCCTGCTTTCCTGGATTCTAGTCTACAGTTTAGCACACTTCTGATTGGCGAGGAACTTGAAGTGTTGCTAATATCTCCGAACGTCTGACTTTTAAAGTTTATATTGGATTTCATATTGGACCGTAGTGTTCTGAAATCAATGAGAAAATGTGGGTTGCGGGTATTTAATTTTCTATGTAAACCATTCTGATTTTGTGGAGGATGAATGAATTTGCTGCTGCTTTCCATGATGGCAAGTAGCTGTTCTAGTTAGCATGTCGAGTGACACCACATGTTTGTGGCAAATTATGGATCGCAGAATTAACGACAATCAAAATTCTGTTGAACTGTTGAATAAAGGTGCTTGCAGGCATATTCTGAAGGTACTA
TARGET=215,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2653
PRIMER_LEFT_PENALTY=0.225177
PRIMER_RIGHT_PENALTY=0.040146
PRIMER_LEFT_SEQUENCE=CCAATTACTTCGTTTCCCGTTA
PRIMER_RIGHT_SEQUENCE=TCCAATGCAATACATCTCCAAG
PRIMER_LEFT=168,22
PRIMER_RIGHT=386,22
PRIMER_LEFT_TM=60.225
PRIMER_RIGHT_TM=59.960
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.2674
PRIMER_LEFT_1_PENALTY=0.227281
PRIMER_RIGHT_1_PENALTY=0.040146
PRIMER_LEFT_1_SEQUENCE=TACCAATTACTTCGTTTCCCGT
PRIMER_RIGHT_1_SEQUENCE=TCCAATGCAATACATCTCCAAG
PRIMER_LEFT_1=166,22
PRIMER_RIGHT_1=386,22
PRIMER_LEFT_1_TM=59.773
PRIMER_RIGHT_1_TM=59.960
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.3273
PRIMER_LEFT_2_PENALTY=0.225177
PRIMER_RIGHT_2_PENALTY=0.102139
PRIMER_LEFT_2_SEQUENCE=CCAATTACTTCGTTTCCCGTTA
PRIMER_RIGHT_2_SEQUENCE=CGAATTTGAAAGGAGAAAGGTG
PRIMER_LEFT_2=168,22
PRIMER_RIGHT_2=323,22
PRIMER_LEFT_2_TM=60.225
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.3294
PRIMER_LEFT_3_PENALTY=0.227281
PRIMER_RIGHT_3_PENALTY=0.102139
PRIMER_LEFT_3_SEQUENCE=TACCAATTACTTCGTTTCCCGT
PRIMER_RIGHT_3_SEQUENCE=CGAATTTGAAAGGAGAAAGGTG
PRIMER_LEFT_3=166,22
PRIMER_RIGHT_3=323,22
PRIMER_LEFT_3_TM=59.773
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=158
PRIMER_PAIR_PENALTY_4=0.3329
PRIMER_LEFT_4_PENALTY=0.225177
PRIMER_RIGHT_4_PENALTY=0.107763
PRIMER_LEFT_4_SEQUENCE=CCAATTACTTCGTTTCCCGTTA
PRIMER_RIGHT_4_SEQUENCE=AGTAGTCCAAATACAAGGCCCA
PRIMER_LEFT_4=168,22
PRIMER_RIGHT_4=420,22
PRIMER_LEFT_4_TM=60.225
PRIMER_RIGHT_4_TM=59.892
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.7000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_04955 Ps_contig_mira-and-tgicl-ass_franssen_35607
SEQUENCE=GCGAGGGGAATTACAATTATTCCATTCCAANATAATAAGTAACTCTCTCGATACACAAACTTCTACAACCAACATTGCACTCATTCATATCTTCTTCTTCTTCAACGTTGTTGTTCTGTCTGATTAATTTCAATGGGAAATCCTCTACAGCTCAAATCACTTAACCACATCTCCTTGGTGTGTAAATCACTAGAAAAATCAGTTGATTTTTACGYCAATGTTCTTGGCTTCGTTCCAATTAAGAGACCTACCTCCTTAAACTTCAATGGTGCATGGTTGTTTAATTATGGCATTGGTATACACCTTCTCCAATCAGATGATCCAGAAGGCATGACCAAGAATCCAGACATAAATCCAAAGGACAACCACATATCTTTCCAAGTATATATCTTTCTCTATTACAATTTTCAACTTTAAATTAAAAGCATATTTATTCATATGGTGTATTTTTTGTTATGTGAATTGTTGTTGGTAGTGTGAGAGTATGGGAGCAGTGGAGAAAAAACTGCAAGAGATGAAGATAGAGTATGTGAAGAATAGAGTTGAGGAAAATGGAATACACGTTGATCAGTTATTCTTCCATGATCCAGATGGATCTATGATTGAAATATGTAATTGTGACAATATTCCGATCGTGCCTTTATCAGAGAATAAAGTATGGTCTTGTTCACGTTTCAACTGTAATATTTCAAACCGGCAGCAGGAGCATCAGATTCAACAA
TARGET=90,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04956 Ps_contig_mira-and-tgicl-ass_franssen_35613
SEQUENCE=CAACAACAACAACAACAGCAAACAGGATCCAGAACTTCTGGTGAACATTAAAACAAAAGAAGTTTGTATTAATCCATGGAGAAGGTTTTGGAGCGTGGTGTTGGTACAAGACTGTTGCGCTTTTAGAAGAAGTAGGATTGCAGCCTGTTGCCTTAGATCTTACAGGATCTGGAATTGATCTCACAGACAGCAACGATGTGACTACATTGGCAGAATATTCAAAACCTTTAACTGTTTATCTTGAAAACCTTCCCGAGGATGAAAAGGTTGTTCTTGTTGGTCATAGCATTGGCGGTGCTTGCATTTCTTATGCATTGGAACATTATCCACAAAAGATCTCAAAAGCGATATTTCTCTGTGCTACAATGGTGACCGATGGTCAAAGACCGTTTGATGTTTTCGCTGATCAGCTAGGATCAGCTGAACAGTTTATGCAAGAATCAAAGTTTCTGATCCATGGTAATGGTAAAGAAAAGCCTCCAACAGGATTTATGTTTGAGAAAGAGCAGATGAAAGGCTTGTATTTTAATCAATCCCCATCAAAGGACGTTGCTTTAGCCATGGTTTCGATGAGACAAAGTCCTATTGGTCCAATCATGGAGAAGCTGTGTTTATCTCCTAACAAATACGGTACTGCTCGCCGTTTTTACATTCAAACATTGGATGATCGCGCTCTTTCACCGGATGTCCAAGAGAAGCTTRGTAAGGGGGAAAATCCACCAGAAGGAGTTTTCAAAATCAAAGGC
TARGET=1,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04957 Ps_contig_mira-and-tgicl-ass_franssen_35638
SEQUENCE=AAATTACGACGTCCTGATGAAGGAGAATCATCCTCTTTCAACATGAGAGTGAGACTTAACCACACAATTCCCATGTAGTTGTTGAATGAAGTGAAAATTTTTCTGTAAATTAGGTTAATCTGCAATGAATGAATGGGTGAATTGGGAGGGATACAAGTATATTTCAAATTGAATGCATGAATGGGTATTTTATATTCACGATTTCCCTTTCTTTCTTCCTCTCTGCGTCAATGTTGAACATTATTATTATGTTTTCTTTCACCTGTATAATAATATTTGAACAAGACTTTTTATTATGCAAATTTATCAAGAACAAAAATATTTATAATTTATCATTTTCATATAATATATATTTTTTAATACATGTAAAAAGTCTAAAATAATAGTGGGTGGTATATTGCAGCAATCTCGTGTGAAATGAAGAATATTATAAATTTCAAGTGTAATTCATTCTAGACAA
TARGET=124,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0999
PRIMER_LEFT_PENALTY=0.081111
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=GACGTCCTGATGAAGGAGAATC
PRIMER_RIGHT_SEQUENCE=ATTGACGCAGAGAGGAAGAAAG
PRIMER_LEFT=7,22
PRIMER_RIGHT=231,22
PRIMER_LEFT_TM=60.081
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.1296
PRIMER_LEFT_1_PENALTY=0.081111
PRIMER_RIGHT_1_PENALTY=0.048460
PRIMER_LEFT_1_SEQUENCE=GACGTCCTGATGAAGGAGAATC
PRIMER_RIGHT_1_SEQUENCE=GGAAGAAAGAAAGGGAAATCGT
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=218,22
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=59.952
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.1504
PRIMER_LEFT_2_PENALTY=0.081111
PRIMER_RIGHT_2_PENALTY=0.069319
PRIMER_LEFT_2_SEQUENCE=GACGTCCTGATGAAGGAGAATC
PRIMER_RIGHT_2_SEQUENCE=AGGAAGAAAGAAAGGGAAATCG
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=219,22
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.069
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=213
PRIMER_PAIR_PENALTY_3=0.2115
PRIMER_LEFT_3_PENALTY=0.081111
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=GACGTCCTGATGAAGGAGAATC
PRIMER_RIGHT_3_SEQUENCE=GACGCAGAGAGGAAGAAAGAAA
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=228,22
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.3914
PRIMER_LEFT_4_PENALTY=0.372557
PRIMER_RIGHT_4_PENALTY=0.018835
PRIMER_LEFT_4_SEQUENCE=AATTACGACGTCCTGATGAAGG
PRIMER_RIGHT_4_SEQUENCE=ATTGACGCAGAGAGGAAGAAAG
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=231,22
PRIMER_LEFT_4_TM=60.373
PRIMER_RIGHT_4_TM=60.019
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_04958 Ps_contig_mira-and-tgicl-ass_franssen_35639
SEQUENCE=AGATTCTCGCTGGGCATGGAGCTCACAGCACTAGTAAAACCCATAGGCACCAGCTTAGAAGCTGCTTCAATGATCTTGTCAACCTTGGCTTCACTGATACCTTTGATTTGCAAAAGATCCTTCCTGGGAGTATAAGCAACAGATTCAACAGTGCAAATACCCGCATCTTTAAGCTTCTTAACATCAATTGCAGCTATACCAGATGTCTGAAGTTGTTCGAACGGGTAAAGGACCGTGTTGAATCTCCTCGTTTCTTGGTCCTGTTGTTGTTGTGCTTGTTTTCTCAAGACGCTGCTGCTCTATTTAACACAACAACGAGTACCGTTGGGTAGGAAGAAAGAAAACGAGGTAGGGTAAAGTTTAGGG
TARGET=262,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1065
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.102139
PRIMER_LEFT_SEQUENCE=ATGATCTTGTCAACCTTGGCTT
PRIMER_RIGHT_SEQUENCE=TCGTTTTCTTTCTTCCTACCCA
PRIMER_LEFT=69,22
PRIMER_RIGHT=346,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=278
PRIMER_PAIR_PENALTY_1=0.1065
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.102139
PRIMER_LEFT_1_SEQUENCE=AATGATCTTGTCAACCTTGGCT
PRIMER_RIGHT_1_SEQUENCE=TCGTTTTCTTTCTTCCTACCCA
PRIMER_LEFT_1=68,22
PRIMER_RIGHT_1=346,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.1244
PRIMER_LEFT_2_PENALTY=0.022302
PRIMER_RIGHT_2_PENALTY=0.102139
PRIMER_LEFT_2_SEQUENCE=AGCTGCTTCAATGATCTTGTCA
PRIMER_RIGHT_2_SEQUENCE=TCGTTTTCTTTCTTCCTACCCA
PRIMER_LEFT_2=59,22
PRIMER_RIGHT_2=346,22
PRIMER_LEFT_2_TM=60.022
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.1314
PRIMER_LEFT_3_PENALTY=0.029253
PRIMER_RIGHT_3_PENALTY=0.102139
PRIMER_LEFT_3_SEQUENCE=TCAATGATCTTGTCAACCTTGG
PRIMER_RIGHT_3_SEQUENCE=TCGTTTTCTTTCTTCCTACCCA
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=346,22
PRIMER_LEFT_3_TM=59.971
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.1372
PRIMER_LEFT_4_PENALTY=0.035021
PRIMER_RIGHT_4_PENALTY=0.102139
PRIMER_LEFT_4_SEQUENCE=GAAGTTGTTCGAACGGGTAAAG
PRIMER_RIGHT_4_SEQUENCE=TCGTTTTCTTTCTTCCTACCCA
PRIMER_LEFT_4=208,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=60.035
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_04959 Ps_contig_mira-and-tgicl-ass_franssen_35641
SEQUENCE=AGCCGCAACAACAACAACTTGTGGTCCCTTYTGATGGAAGGCCACCACCACCTTTCATTATGGGCTCCATCTCCAATATTCAACCTTCTTCAAACTCTTCATCTTCTTCCTCAAATTCTTCCTCTTCTTCCGCCTCAGCTTCTGCAACTACGCCTCATCTTATTGACGCTTCTCTCGCAATCGCCACCAGATCTGAACCTCCGGTACTCGACTCCACCGCTGTAGCCAACAACAATAACAGTAATAGTCAGATTCAACAATTCTCTTCTTCATCCACAGCCGTCACGGTAGCCAATCCTCCCAAACGATCCACTAAGGACAGACACACGAAAGTCGATGGCCGGGGACGAAGAATTCGAATGCCGGCGACATGTGCAGCTAGAGTTTTTCAGTTGACCAGAGAATTAGGCCATAAATCCGACGGTGAAACAATTGAATGGCTTCTCCAGCAAGCGGAACCGGCTATTATTGCCGCCACCGGCACAGGAACAATCCCGGCTAATTTCTCAACACTCAACAATGTTTCACTTCGAAGCAGCGGCTC
TARGET=6,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04960 Ps_contig_mira-and-tgicl-ass_franssen_35658
SEQUENCE=CTGTTTACTTTAACTATAATTTAAAYTAACNTAGTAAAAACAATGGGGTCATGTTTAAATAATTGGCAGGAATGGGTCGAATAATGGCTTCAAATAGCCAACAGTATATATATATTTACAAAACTATCTATCTGAAAGGAAGAAGAAAAAAAAGACGAGGAGAGAAGGTTAGGAGGTTTCCGCTTTCATGATGGACTTTTTCTCAAGAGAAATTTGTGGGGGGTGGATTATTGATGATTCGACATGGAGGCACCATTTTATGAAGCATGTTGGTCCCTTTGAAGTTCACAAATTGATTGCAGTCCCTTCTGTAGCTGGTAAGCTGGTCTGGTTTAATCTTGCTAAACCCGAATTTATCTATCCATATGGACTGTGCTTCTTCTGCAGCTGGTAGCACAAGGTTCTTTACTTTCATAAAAGCCAGTAGCCTTTCGATGCAGGAGAAGAGTGTTTGGAAGTAGCCCTTCCCATGATGACTGTTACTTGTTGCAACTAAAGGAAGTTCAGCAATATCAGTGCCGAAGATTCGAATCATGCCCGCAGATACCACAGATGAATTGACTATTAGTAATGCACAATGCATTCCTCCAAACTCTTGACCCCGCACACTCTTTCCATAAACCATTGCTCTAATAAGGTCACGGCCGCTAGCTGCATCAACTATAGGATCAAAGCATTGATGAAAGATAGAGACAGCCTCCAAAAGCAATGGTCTAGTTTCAGGAGAAGCATTTTTTCCACTAAGAAGCCTCCAACTCACGTTAATATCATTGATGGGATCCAATCCTTTTCTCCTTGCTTCTTCTTTATAAGAGCCAAGAGAGATTGAGGGAGTCTCTCAGCCCCTCTAACTAAAACATTCCTCAAAGTAGAATGTATTCTCTTGCAATCA
TARGET=105,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04961 Ps_contig_mira-and-tgicl-ass_franssen_35669
SEQUENCE=ACANGTTTTTYRGAGATCAAAAGCAAAATTCCCAATATTTCTTTAATGGAAAATTGGAGGCCTCTAAAAAGACCAAAAGTGGAAGAAGAAGATACAATGAAAATGTAAGGGCTTGGAGCAATGCAACTCCTCAACAATTACAATTGAAATTGCAAGCTCCATCTTTGATTCATGTGTGATGATAACTCTCCTAACAAGTATCTTTATCCAACAATCCAACAACAACAACAACAGCATAACTTCAACATCCATATAACCAAACCCCAAAAGCAGTATAAAGAATTCAAGTATCTTCTTGATGGGGCTTATTGTAACTTCCACATTATGATTCTTCTTCATTCATCAATCAAATCAATTGTTGGAATCAAAATACTAAAAAATATATACAGGAGAGACTTGCACCTCAATCCCATTTTCACATCTTGGGGGAGTTATTGAAGTTCTGTGATCTGAGAAGCTGTGCTAACCTTTACTATCTTGCTCCTTTGGAATATACAAAATTATCGGCACCGCCGCCGAGTTTAATAATCCAGTGTCATTGGATGGTTCTTGTAAGCTTGCACTTGGAAATGAAGATAGTATCTGAATAATTCAAACTACACAGTTTCAAAAGCTCGGCGGATTGTTGTTTTAGTATTGGATTGCAACCACTTTGAATCACTAGAAGAAGTTTCGCAGCAAGCCCTGCGTCGATGGCAATCGCCGAACACTCCTCCGGCGCAAGCTTGCAAACAGACCACAGAATCGACAACGCGTACTGAGTGCATGTTTCTGAGATCCTCATCAAAAACCTCACCATGATAGGTATCGTGTTGACACAATCCCTCAAAGCGACTCTGCCTTCGGGTATGGACGACAACGAATCCAAAACACAAAGCGCTAATTCCAAACAATCATGGTCCATACCAGACAACAATTCGACTAATTGAGAAACAGCACCAATGCTAACTAACAAAATCTTAACTTCTTTATAGAAACACACTGTTTTGAGGAGGCTTAATCCAGGACAGATTCCATTACTATGTCTCTTATCCTTGACAAGCCTCATCAAA
TARGET=217,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04962 Ps_contig_mira-and-tgicl-ass_franssen_35672
SEQUENCE=AAATTGATTGACAAAGCAAAGCATAAAAGAACTTGTTGTAAGAGATTCTTTTCCCAAAACTCAGAACAACCAGTATTCTTTGTCTCTCTCTCTTTCCAATGGTTTCTAACAAAAGGGTTACTGCATTACTACTGATAATGCTGGCCATTCTAGCTAACTCAGATTTATCAAAAGACAGAGAAGAATGTGCTGACAAACTTGTTACTCTTGCTAGTTGTCTTCCATACGTTGGTGGTGATGCCAACACTCCCACCATAGATTGTTGTACATCCCTCAAAGTAGTCCTTGACAAGACCAAAAAATGCATCTGCATCCTCATCAAAGATCGTAACGACCCCAAACTCGGCTTCACCTTGAATGCAACTCTCGCTGTTCACCTTCCAACTGCTTGTCACATACCATCTAATATATCTCAATGTGTAGATCTTTTGCATTTGTCGCCAAAATCTCCTGAAGCTCAGGTGTTTGAAGGACTTGGAAATTCTACCAAAACAAACAGTTCCACAGCAGGTTCTTCTGTTGAGAAGGGATCAAGTTCAGAGGAAAAGAGTGGTGGTGCTTTGGGAAAGAGGTGGGTGGTGGCAGAGATGGTTTGTTTGTCTATTTTACCATTTGTTTTCATATCTCACTTCTTCATCATAGCATGAACCATGATCAAGATTTAGAAAAGACTCGTTACTTTGATTTTTTCATGTATGAATCTAATACTCATTGCTCATTAGTGGTATTTATTTCTCATACTTTTGTTATGTTTGTTGTTTCTTTAAGTCCTTTATTTACTTTTTTAGTCAAATTCATGTTTGTGTACT
TARGET=83,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6168
PRIMER_LEFT_PENALTY=0.364634
PRIMER_RIGHT_PENALTY=0.252154
PRIMER_LEFT_SEQUENCE=TTTCCCAAAACTCAGAACAACC
PRIMER_RIGHT_SEQUENCE=AGCTAGAATGGCCAGCATTATC
PRIMER_LEFT=49,22
PRIMER_RIGHT=154,22
PRIMER_LEFT_TM=60.365
PRIMER_RIGHT_TM=59.748
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=0.6570
PRIMER_LEFT_1_PENALTY=0.364634
PRIMER_RIGHT_1_PENALTY=0.292414
PRIMER_LEFT_1_SEQUENCE=TTTCCCAAAACTCAGAACAACC
PRIMER_RIGHT_1_SEQUENCE=TCGTTACGATCTTTGATGAGGA
PRIMER_LEFT_1=49,22
PRIMER_RIGHT_1=333,22
PRIMER_LEFT_1_TM=60.365
PRIMER_RIGHT_1_TM=59.708
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.6795
PRIMER_LEFT_2_PENALTY=0.427382
PRIMER_RIGHT_2_PENALTY=0.252154
PRIMER_LEFT_2_SEQUENCE=CCCAAAACTCAGAACAACCAGT
PRIMER_RIGHT_2_SEQUENCE=AGCTAGAATGGCCAGCATTATC
PRIMER_LEFT_2=52,22
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=60.427
PRIMER_RIGHT_2_TM=59.748
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.7198
PRIMER_LEFT_3_PENALTY=0.427382
PRIMER_RIGHT_3_PENALTY=0.292414
PRIMER_LEFT_3_SEQUENCE=CCCAAAACTCAGAACAACCAGT
PRIMER_RIGHT_3_SEQUENCE=TCGTTACGATCTTTGATGAGGA
PRIMER_LEFT_3=52,22
PRIMER_RIGHT_3=333,22
PRIMER_LEFT_3_TM=60.427
PRIMER_RIGHT_3_TM=59.708
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=0.9233
PRIMER_LEFT_4_PENALTY=0.364634
PRIMER_RIGHT_4_PENALTY=0.558648
PRIMER_LEFT_4_SEQUENCE=TTTCCCAAAACTCAGAACAACC
PRIMER_RIGHT_4_SEQUENCE=AGTTAGCTAGAATGGCCAGCAT
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=158,22
PRIMER_LEFT_4_TM=60.365
PRIMER_RIGHT_4_TM=59.441
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_04963 Ps_contig_mira-and-tgicl-ass_franssen_35692
SEQUENCE=ACCACAAAACAAAACAAACAAACAACAAAAAACCAAACTTTTTTTAGTTTCTTTTGGTAAGTCTTGAGATTCTTTCCTATGAACGAAGCTCAARCTGAAATCACAATTCCTCACTTGTTTAGATGCCCCATAAGTCTTGATTTGTTGGAAGATCCAGTGACTTTAACCACTGGGCAAACCTATGATAGATCAAGCATAGAGAAATGGATTTCCGCAGGTAATTTCACATGTCCTGTAACAATGCAGAAGCTTCATGATTTATCTTTTGTTCCTAATCACACTCTTCGCCATTTGATTAATCAATGGCTTCAGCTTGGTTCACAATTTCATCACCCTTGTAATAATAACATCTCTGCAACCATAGATTATTTAGCTACAATAAACACACCCTTGAATCTCATGATTCATCTATAAGAAACCAAGTTAGAAAGCACTTAGAA
TARGET=4,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04964 Ps_contig_mira-and-tgicl-ass_franssen_35692
SEQUENCE=ACCACAAAACAAAACAAACAAACAACAAAAAACCAAACTTTTTTTAGTTTCTTTTGGTAAGTCTTGAGATTCTTTCCTATGAACGAAGCTCAARCTGAAATCACAATTCCTCACTTGTTTAGATGCCCCATAAGTCTTGATTTGTTGGAAGATCCAGTGACTTTAACCACTGGGCAAACCTATGATAGATCAAGCATAGAGAAATGGATTTCCGCAGGTAATTTCACATGTCCTGTAACAATGCAGAAGCTTCATGATTTATCTTTTGTTCCTAATCACACTCTTCGCCATTTGATTAATCAATGGCTTCAGCTTGGTTCACAATTTCATCACCCTTGTAATAATAACATCTCTGCAACCATAGATTATTTAGCTACAATAAACACACCCTTGAATCTCATGATTCATCTATAAGAAACCAAGTTAGAAAGCACTTAGAA
TARGET=12,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04965 Ps_contig_mira-and-tgicl-ass_franssen_35702
SEQUENCE=CCGTTCCTACGAAGGTTTACCTTCCTTAAAACCTTAACCCCTAAACCCCAAAAACCCAAAAACCCCCCTAACCCTTACCCTAAACCAAGACATACTATCTAATAACCTAAGTATACTATTGAATGGTACTAAACTNACTAANCATGTATAGTATTCCTAAATTGAAGACTTCAACATGTTGCTGCTCTAGTGGATTTGAGCACCAGTGTATGTTTGACCAAATGACCATCCAGCTGGAGCAACATTATTGGAGACCACAGTTCGACCATCACTTGTTGTAACCTTAAAAGACAAGCTTTGACCGTTAAGATAATTGTTGCTCTGCCAGTTCTGTCCCCAGTTTCTTGACATCGAAATCCAACCAGTTTTTGACCCTTTGATGAAAGCAGCAACCACATCACCAGCACCACCAACGTTTGTGATAAGAACAAGGTTGAAGTAAGAATGTCCATTGATAGTGAACCTTATACCTCCCTTTCTCCTGCAGGATACCCTTCTGTAAGATACAGGAACGATTCCAGCTTTGTATTGAGCAATATGAAGAAAAACAGGTTGAGCAAGATCAAAATGTTGGAGAGGAGGGTTACACCAACCACCTGCATTATTAGGCAAGGCATTGTTTGGTGGACAGAAGTTGGTAGCAGTGACAACAATGGAGCCAGGAAGGCACCATTTATGGTCACCTGCACATTTAATTTCATAGCAAGAGCCACAGCTTAAACCGTTGTTGAAAAGTGCTGTGCTCAAAGCAGCAGTGTTTGTTCCATACCCTTGACTGTAAAGGTTACCATATCCACAAGCCCCACCCATTGTTCCTGATGCATCACTGCCTCCATAGAAGGTAGCATGAGCATTAGTCCATGCTTCAGCATAGGCATGAACAGATGAGAACATAGTGAGGGAACACACCAAAAGCAAGCCAATGAAAGCCATTTCTTGTTTCTTTGAGGAGTAGAAGGTAGTTAGAGAGAGAGAGCAATTAGTTTGATGAGTGAGTC
TARGET=965,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04966 Ps_contig_mira-and-tgicl-ass_franssen_35708
SEQUENCE=AGTTTTGATACTCCACTCGAACATAGTCTTCTTTCCTTTCGACTATTCTTGGTGTAAATTTGTCCGGTCTTGTTGATTCTATCACTTCTATTAGTTCCTCCATTGCCTCTTCTCTGCTCACAGGACTTTTCCTGCCTTCAGGGTTATAGTTCCAAGGAGGAGCATAATGAATGAGATCACTGACATTCTCAGAGGTTGACACACAATTCTTAGTTGCTGGACATAGAGCCAATGCTGATGAACTTTTCTGAACTCCAAGATATTCAGGCTTTTTCCCACTTAAATTGAAGATGGCGCCAATGGTAGCTAACTCGCTGCTTCTCAAAATGAGCTGTCTTCGATTGATTTGATGGGTAGCGTTGTTGTTCTCCTCCTCCTGCTGCTTCATGCGAAGAACACGAGGAAGAGAGAAAGTTTTGTTATTATTGATTGGTATTTTGAGGTGGAACTTGAGGTTGCAGAATGTGGTTGAAGAAGAAGAAGATGCCATTGTAAGGGTAGAAACCTAAACTTGAGTTGAGTTGAAAAATGAAATCAGTATATTTTGTTATGAGAATGCAAATGATAAGTTTGACCTCAAAACTCCAAATATTATAAGAATGGATGAAATGAAACATGGAAAATAAACTCCCCC
TARGET=471,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4671
PRIMER_LEFT_PENALTY=0.183585
PRIMER_RIGHT_PENALTY=0.283555
PRIMER_LEFT_SEQUENCE=GTAGCGTTGTTGTTCTCCTCCT
PRIMER_RIGHT_SEQUENCE=GGGGGAGTTTATTTTCCATGTT
PRIMER_LEFT=353,22
PRIMER_RIGHT=633,22
PRIMER_LEFT_TM=59.816
PRIMER_RIGHT_TM=60.284
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.4947
PRIMER_LEFT_1_PENALTY=0.211181
PRIMER_RIGHT_1_PENALTY=0.283555
PRIMER_LEFT_1_SEQUENCE=GATTGATTTGATGGGTAGCGTT
PRIMER_RIGHT_1_SEQUENCE=GGGGGAGTTTATTTTCCATGTT
PRIMER_LEFT_1=339,22
PRIMER_RIGHT_1=633,22
PRIMER_LEFT_1_TM=60.211
PRIMER_RIGHT_1_TM=60.284
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=0.5034
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.283555
PRIMER_LEFT_2_SEQUENCE=GAACTTGAGGTTGCAGAATGTG
PRIMER_RIGHT_2_SEQUENCE=GGGGGAGTTTATTTTCCATGTT
PRIMER_LEFT_2=445,22
PRIMER_RIGHT_2=633,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=60.284
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.8205
PRIMER_LEFT_3_PENALTY=0.536975
PRIMER_RIGHT_3_PENALTY=0.283555
PRIMER_LEFT_3_SEQUENCE=GGAACTTGAGGTTGCAGAATGT
PRIMER_RIGHT_3_SEQUENCE=GGGGGAGTTTATTTTCCATGTT
PRIMER_LEFT_3=444,22
PRIMER_RIGHT_3=633,22
PRIMER_LEFT_3_TM=60.537
PRIMER_RIGHT_3_TM=60.284
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=190
PRIMER_PAIR_PENALTY_4=0.8205
PRIMER_LEFT_4_PENALTY=0.536975
PRIMER_RIGHT_4_PENALTY=0.283555
PRIMER_LEFT_4_SEQUENCE=GTGGAACTTGAGGTTGCAGAAT
PRIMER_RIGHT_4_SEQUENCE=GGGGGAGTTTATTTTCCATGTT
PRIMER_LEFT_4=442,22
PRIMER_RIGHT_4=633,22
PRIMER_LEFT_4_TM=60.537
PRIMER_RIGHT_4_TM=60.284
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_04967 Ps_contig_mira-and-tgicl-ass_franssen_35717
SEQUENCE=CTTTTAAAACTTACCTTTTCGTTTGGTTTTTCGTTTTTATTAAGGTTTTACGTTTACTTTGTAAATTAGGAGGTATGAGAATCGATTCTAGACAAGACTCAAAGACAAAAATAATAGATTGTCTTCTTTTTGGATTTAGATGATACTTTGTATCCACTAAGCTGTGGTATTGCAAAAGCATGTGGCCAAAACATTAAAGACTATATGGTTGAGAAGCTTGGCATAGACAGAAACATAATTGATGACATGTCAAACCATCTTTACAAAAACTATGGAACAACTATGGCTGGTTTAAGGGCAATTGGATATGACTTTGACTATGATGAATATCATAGTTTTGTTCATGGAAGATTACCATATGAGAATTTGAAACCAGACCCAGTTTTAAGGAACCTCTTGTTGAGTCTACCTTATAGAAAACTAATCTTCACAAATGCAGACAAAGTTCATGCAGTTAAGGCACTAAGCAAGCTTGGATTAGAAGATTGTTTTGAAGGAATTATATGTTTTGAGACACTTAATCCTATCCACAAAAACACTGTATCTGATGATGATGAAGATGACATTGAGTTTATAGGTTCAAAGAGAACCACTCACACAACAAGTACTAGTTCAAGTAACTTGCAAATATTTGACATTATTGCCCATTTTGCACAATCCAATCCAACTCAAGCTTTGCCAAAGACACCAATTATTTGCAAACCTTCAGAATTTGCCATTGAGTTGGCTCTCAAGATTGCTAATCTGGACCCACAAAGAACCTTATTCTTTGAGGATAGTGCTAGAAACATACAAGCTGGAAAACGTGTAGGACTTGACACAGTGTTGGTTGGAAAATCACAAAGAATTAAAGGTGCAGATTATGCCTTAGAAAGCATCCACAACTTAAGGGAGGCAGTGCCAGAATTATGGGAATCAGAACTTAAATCAGAAGTTGCTTATCCTAGCAACCTTGCTGTGGAGACATCTGTGACTGCTTAATTAGAATTAATTAACTATTTAATTACAAGGAATTAAGCCACTTAATCATTGATGTCAAAAAACAAAAACAAAAAATTCAAG
TARGET=546,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2767
PRIMER_LEFT_PENALTY=0.272307
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=TGCAGTTAAGGCACTAAGCAAG
PRIMER_RIGHT_SEQUENCE=AGCTTGAGTTGGATTGGATTGT
PRIMER_LEFT=449,22
PRIMER_RIGHT=674,22
PRIMER_LEFT_TM=59.728
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=226
PRIMER_PAIR_PENALTY_1=0.3173
PRIMER_LEFT_1_PENALTY=0.312895
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=AATGCAGACAAAGTTCATGCAG
PRIMER_RIGHT_1_SEQUENCE=AGCTTGAGTTGGATTGGATTGT
PRIMER_LEFT_1=432,22
PRIMER_RIGHT_1=674,22
PRIMER_LEFT_1_TM=60.313
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.4416
PRIMER_LEFT_2_PENALTY=0.437208
PRIMER_RIGHT_2_PENALTY=0.004359
PRIMER_LEFT_2_SEQUENCE=TCATGCAGTTAAGGCACTAAGC
PRIMER_RIGHT_2_SEQUENCE=AGCTTGAGTTGGATTGGATTGT
PRIMER_LEFT_2=446,22
PRIMER_RIGHT_2=674,22
PRIMER_LEFT_2_TM=59.563
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=0.4532
PRIMER_LEFT_3_PENALTY=0.448819
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=CATGCAGTTAAGGCACTAAGCA
PRIMER_RIGHT_3_SEQUENCE=AGCTTGAGTTGGATTGGATTGT
PRIMER_LEFT_3=447,22
PRIMER_RIGHT_3=674,22
PRIMER_LEFT_3_TM=60.449
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=0.5696
PRIMER_LEFT_4_PENALTY=0.565196
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=GCAGTTAAGGCACTAAGCAAGC
PRIMER_RIGHT_4_SEQUENCE=AGCTTGAGTTGGATTGGATTGT
PRIMER_LEFT_4=450,22
PRIMER_RIGHT_4=674,22
PRIMER_LEFT_4_TM=60.565
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_04968 Ps_contig_mira-and-tgicl-ass_franssen_35719
SEQUENCE=ACAGAGAATCGAAATAAAAATAAATAAATAATTTTTTTTCTTAAATTAACAACTGGCATTTTCTAAAGCAGCACCCAACAAAATCCACATATAATCAGAAACTTGAGAAATGATCCTGAGAAGGTTGAACCATTTGCATGTCTGTGGTTGTTTGGCATGAGTTATCATCACTCATCATGGTCTCTCGTGGTCTTGATGCCCAAAGAGAACTAAGAGCTCGAAGTCGACCGTAAATATTCTCCGATAACAAGAAAAGAACGCGCA
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04969 Ps_contig_mira-and-tgicl-ass_franssen_35721
SEQUENCE=AGTGTCATAAACAAGGATTCTTCTTCTTCTTCAGTTTCCTCAAACAATATGGGTTCTTTTCCGAGTCCAGGAGCACCGTATTACAGAGAGAGAACAAGAAGGTATGAGAACAACAAGGGATGGAGCTCAGAGAGAGTATCGAAGCAGACAAGCAGCAGCAGCAGAAGACATACGATGTCTGGTTTAACACCATTCAGTGGTGGAAGAACAATGCCATCAAAATGGGATGAAGCTGAGAGATGGATTTGTAGTCCAGTTTCAGCATCATATGTTGATAGCAGAAGAAATTCACATACACAACAACAACTTCTTCAACAGCGAAGACCTAAGTCAATAAGTGGCCCAATTGTTCCTCCTCCTGGAGTGGCTTTTTACTCGAATTATTCACCGTCTGCTCAACAACTGAGATAAGGTTTTGATGTGAGGAA
TARGET=18,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04970 Ps_contig_mira-and-tgicl-ass_franssen_35721
SEQUENCE=AGTGTCATAAACAAGGATTCTTCTTCTTCTTCAGTTTCCTCAAACAATATGGGTTCTTTTCCGAGTCCAGGAGCACCGTATTACAGAGAGAGAACAAGAAGGTATGAGAACAACAAGGGATGGAGCTCAGAGAGAGTATCGAAGCAGACAAGCAGCAGCAGCAGAAGACATACGATGTCTGGTTTAACACCATTCAGTGGTGGAAGAACAATGCCATCAAAATGGGATGAAGCTGAGAGATGGATTTGTAGTCCAGTTTCAGCATCATATGTTGATAGCAGAAGAAATTCACATACACAACAACAACTTCTTCAACAGCGAAGACCTAAGTCAATAAGTGGCCCAATTGTTCCTCCTCCTGGAGTGGCTTTTTACTCGAATTATTCACCGTCTGCTCAACAACTGAGATAAGGTTTTGATGTGAGGAA
TARGET=151,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0627
PRIMER_LEFT_PENALTY=0.020747
PRIMER_RIGHT_PENALTY=0.041981
PRIMER_LEFT_SEQUENCE=GGGATGGAGCTCAGAGAGAGTA
PRIMER_RIGHT_SEQUENCE=TCGCTGTTGAAGAAGTTGTTGT
PRIMER_LEFT=116,22
PRIMER_RIGHT=320,22
PRIMER_LEFT_TM=59.979
PRIMER_RIGHT_TM=59.958
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.0729
PRIMER_LEFT_1_PENALTY=0.020747
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=GGGATGGAGCTCAGAGAGAGTA
PRIMER_RIGHT_1_SEQUENCE=TGACTTAGGTCTTCGCTGTTGA
PRIMER_LEFT_1=116,22
PRIMER_RIGHT_1=332,22
PRIMER_LEFT_1_TM=59.979
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.0924
PRIMER_LEFT_2_PENALTY=0.020747
PRIMER_RIGHT_2_PENALTY=0.071684
PRIMER_LEFT_2_SEQUENCE=GGGATGGAGCTCAGAGAGAGTA
PRIMER_RIGHT_2_SEQUENCE=GGTCTTCGCTGTTGAAGAAGTT
PRIMER_LEFT_2=116,22
PRIMER_RIGHT_2=325,22
PRIMER_LEFT_2_TM=59.979
PRIMER_RIGHT_2_TM=59.928
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.1498
PRIMER_LEFT_3_PENALTY=0.020747
PRIMER_RIGHT_3_PENALTY=0.129103
PRIMER_LEFT_3_SEQUENCE=GGGATGGAGCTCAGAGAGAGTA
PRIMER_RIGHT_3_SEQUENCE=ATTCGAGTAAAAAGCCACTCCA
PRIMER_LEFT_3=116,22
PRIMER_RIGHT_3=380,22
PRIMER_LEFT_3_TM=59.979
PRIMER_RIGHT_3_TM=60.129
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=265
PRIMER_PAIR_PENALTY_4=0.1964
PRIMER_LEFT_4_PENALTY=0.020747
PRIMER_RIGHT_4_PENALTY=0.175676
PRIMER_LEFT_4_SEQUENCE=GGGATGGAGCTCAGAGAGAGTA
PRIMER_RIGHT_4_SEQUENCE=GTTGTTGAGCAGACGGTGAATA
PRIMER_LEFT_4=116,22
PRIMER_RIGHT_4=402,22
PRIMER_LEFT_4_TM=59.979
PRIMER_RIGHT_4_TM=60.176
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_04971 Ps_contig_mira-and-tgicl-ass_franssen_35729
SEQUENCE=ATCTTCCTTAGGTGTAGCTCGATCAACCACTACAGCAGTACCTCCGAGTTCATAGATTTCTGACATCAAACTCTCCACAGATTCTGCATTTGCAAAAGTGATAAAACCAATTCCACGATGCATCTTTGAGCCTTGATCCTTTGGCATGTACAAATCTGTTATATCTCCATATTTCTCAAAATGACTTCTAAAAGTTGTTTCTGTTACTGATTGTGGAATCCTGGCCACAAAAATTCTGGTAACTTTTTTGGCTGGTGCCCTCATCTCCTCCTTTGGTGTAGCCACTTTGACTTCAAGCATCCTATTACCAAGAACATGTTCACCTGACAATACTTCCTTGGCATCATCTACTGAAGCAAATGTAACATATCCAAAACCACGAGATCGTCCAGTTGAACGCTCCTTCATGACAATGCAATCTTCTAGTTCACCATATTTGCTCATGTATTCTTTCAAGCCCTCAGTGTCAACATCCCAAGGGATACCCAAAACCACAAGTTTTCTCTGCTCCATCTGAAACTTAACAAAGCGCAAAGAAATAGATTGAAACGCTGTAGATGAAAAAAAATCCCTATGCACGAGTCTGAAAGGGAAATTAACAAGAAAAAGAAAAAACTATATCCACCTCGTTCTCTTGGGGTAATGGTATCTCTCAGCGTTCTCACACCCCTCTCTCTAAAAATCTCACACTTTCTCCTCCCTTGATGAAAACGAAACTCAAAACCCTAAATTGAAGATCTCGCTAGCTAGATCGCTCTATCTATCTCCCTCTTCCTCCCTCGCTCGCTCGCTCCGCACCGTCTCAGCCTCCTGTTTGTCCCCGAG
TARGET=779,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3257
PRIMER_LEFT_PENALTY=0.110131
PRIMER_RIGHT_PENALTY=2.215563
PRIMER_LEFT_SEQUENCE=ACACTTTCTCCTCCCTTGATGA
PRIMER_RIGHT_SEQUENCE=GGACAAACAGGAGGCTGAGAC
PRIMER_LEFT=686,22
PRIMER_RIGHT=819,21
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=61.216
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=57.143
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=2.3257
PRIMER_LEFT_1_PENALTY=0.110131
PRIMER_RIGHT_1_PENALTY=2.215563
PRIMER_LEFT_1_SEQUENCE=TCACACTTTCTCCTCCCTTGAT
PRIMER_RIGHT_1_SEQUENCE=GGACAAACAGGAGGCTGAGAC
PRIMER_LEFT_1=684,22
PRIMER_RIGHT_1=819,21
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=61.216
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=57.143
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=2.4959
PRIMER_LEFT_2_PENALTY=0.110131
PRIMER_RIGHT_2_PENALTY=2.385756
PRIMER_LEFT_2_SEQUENCE=ACACTTTCTCCTCCCTTGATGA
PRIMER_RIGHT_2_SEQUENCE=GGACAAACAGGAGGCTGAGA
PRIMER_LEFT_2=686,22
PRIMER_RIGHT_2=819,20
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.386
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=2.5543
PRIMER_LEFT_3_PENALTY=0.110131
PRIMER_RIGHT_3_PENALTY=2.444121
PRIMER_LEFT_3_SEQUENCE=ACACTTTCTCCTCCCTTGATGA
PRIMER_RIGHT_3_SEQUENCE=ACAAACAGGAGGCTGAGACG
PRIMER_LEFT_3=686,22
PRIMER_RIGHT_3=817,20
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=60.444
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=2.5543
PRIMER_LEFT_4_PENALTY=0.110131
PRIMER_RIGHT_4_PENALTY=2.444121
PRIMER_LEFT_4_SEQUENCE=TCACACTTTCTCCTCCCTTGAT
PRIMER_RIGHT_4_SEQUENCE=ACAAACAGGAGGCTGAGACG
PRIMER_LEFT_4=684,22
PRIMER_RIGHT_4=817,20
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=60.444
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_04972 Ps_contig_mira-and-tgicl-ass_franssen_35742
SEQUENCE=CATGCAATTGCACTTACTTGACGTCATATATGCTTATTGTTTGTAAATAGCGCCATGTTGCGGCACTATAGTACCAGAGAGGAGCAGCGTGGGTGTGCCCATATGTGGCGGTGCAGCACAGAAGTAAGTCTAGAAGTCTTTTTGCGCAGATAGCCGTCCCACCACGACCCACTCTGGATTAGAAGAAAAGAAAAATCAAAGAAAGAAAGTTATTTTTTACTTGTTGAGTGGCATATACTTAACCTTACCTAGTAGGAACTAAATTAACTTTTTACCTTTTTTTTCCTTACGTCG
TARGET=198,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.7590
PRIMER_LEFT_PENALTY=0.060824
PRIMER_RIGHT_PENALTY=4.698171
PRIMER_LEFT_SEQUENCE=ATAGTACCAGAGAGGAGCAGCG
PRIMER_RIGHT_SEQUENCE=GGTTAAGTATATGCCACTCAACAAG
PRIMER_LEFT=67,22
PRIMER_RIGHT=243,25
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=58.302
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=5.1976
PRIMER_LEFT_1_PENALTY=0.060824
PRIMER_RIGHT_1_PENALTY=5.136785
PRIMER_LEFT_1_SEQUENCE=ATAGTACCAGAGAGGAGCAGCG
PRIMER_RIGHT_1_SEQUENCE=GGTAAGGTTAAGTATATGCCACTCA
PRIMER_LEFT_1=67,22
PRIMER_RIGHT_1=248,25
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=57.863
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=5.2866
PRIMER_LEFT_2_PENALTY=0.588441
PRIMER_RIGHT_2_PENALTY=4.698171
PRIMER_LEFT_2_SEQUENCE=TAGTACCAGAGAGGAGCAGCGT
PRIMER_RIGHT_2_SEQUENCE=GGTTAAGTATATGCCACTCAACAAG
PRIMER_LEFT_2=68,22
PRIMER_RIGHT_2=243,25
PRIMER_LEFT_2_TM=60.588
PRIMER_RIGHT_2_TM=58.302
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=176
PRIMER_PAIR_PENALTY_3=5.7252
PRIMER_LEFT_3_PENALTY=0.588441
PRIMER_RIGHT_3_PENALTY=5.136785
PRIMER_LEFT_3_SEQUENCE=TAGTACCAGAGAGGAGCAGCGT
PRIMER_RIGHT_3_SEQUENCE=GGTAAGGTTAAGTATATGCCACTCA
PRIMER_LEFT_3=68,22
PRIMER_RIGHT_3=248,25
PRIMER_LEFT_3_TM=60.588
PRIMER_RIGHT_3_TM=57.863
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=5.7668
PRIMER_LEFT_4_PENALTY=1.068602
PRIMER_RIGHT_4_PENALTY=4.698171
PRIMER_LEFT_4_SEQUENCE=TATAGTACCAGAGAGGAGCAGCG
PRIMER_RIGHT_4_SEQUENCE=GGTTAAGTATATGCCACTCAACAAG
PRIMER_LEFT_4=66,23
PRIMER_RIGHT_4=243,25
PRIMER_LEFT_4_TM=60.069
PRIMER_RIGHT_4_TM=58.302
PRIMER_LEFT_4_GC_PERCENT=52.174
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_04973 Ps_contig_mira-and-tgicl-ass_franssen_35758
SEQUENCE=CCGGTTTGGTTCGTTCCTAGAAAACCGGAAACGGAAAAGGAAAAGTAAGGTAAAAAGGAACGTCTTGTATGGCGGAATAGGTGTCGGCGTGGACGGTGTCGTTGCTAAGAACGGTAAGCGGGAACGTTACGTACGGTGGATGGATGCAGTGCATGCAGCTCCTGATAATAGCATGAAACGGTTTGTGATTTATGTGAAGAGAGGTGTTTATGTTGAAAATGTTGAAATAAAGAAGAAGAAGTGGAATCTTATGATGGTTGGAGATGGTATGAATGCGAGTGTTATATCGGGTAATCGGAGCTTCATCGACGG
TARGET=230,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4467
PRIMER_LEFT_PENALTY=0.258911
PRIMER_RIGHT_PENALTY=0.187835
PRIMER_LEFT_SEQUENCE=GTGCATGCAGCTCCTGATAATA
PRIMER_RIGHT_SEQUENCE=GCTCCGATTACCCGATATAACA
PRIMER_LEFT=148,22
PRIMER_RIGHT=300,22
PRIMER_LEFT_TM=60.259
PRIMER_RIGHT_TM=60.188
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.2000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.4506
PRIMER_LEFT_1_PENALTY=0.258911
PRIMER_RIGHT_1_PENALTY=0.191680
PRIMER_LEFT_1_SEQUENCE=GTGCATGCAGCTCCTGATAATA
PRIMER_RIGHT_1_SEQUENCE=TTCATACCATCTCCAACCATCA
PRIMER_LEFT_1=148,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=60.259
PRIMER_RIGHT_1_TM=60.192
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=0.4902
PRIMER_LEFT_2_PENALTY=0.258911
PRIMER_RIGHT_2_PENALTY=0.231280
PRIMER_LEFT_2_SEQUENCE=GTGCATGCAGCTCCTGATAATA
PRIMER_RIGHT_2_SEQUENCE=ACCCGATATAACACTCGCATTC
PRIMER_LEFT_2=148,22
PRIMER_RIGHT_2=291,22
PRIMER_LEFT_2_TM=60.259
PRIMER_RIGHT_2_TM=60.231
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=0.5071
PRIMER_LEFT_3_PENALTY=0.315379
PRIMER_RIGHT_3_PENALTY=0.191680
PRIMER_LEFT_3_SEQUENCE=GTCGTTGCTAAGAACGGTAAGC
PRIMER_RIGHT_3_SEQUENCE=TTCATACCATCTCCAACCATCA
PRIMER_LEFT_3=97,22
PRIMER_RIGHT_3=272,22
PRIMER_LEFT_3_TM=60.315
PRIMER_RIGHT_3_TM=60.192
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=0.5467
PRIMER_LEFT_4_PENALTY=0.315379
PRIMER_RIGHT_4_PENALTY=0.231280
PRIMER_LEFT_4_SEQUENCE=GTCGTTGCTAAGAACGGTAAGC
PRIMER_RIGHT_4_SEQUENCE=ACCCGATATAACACTCGCATTC
PRIMER_LEFT_4=97,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.315
PRIMER_RIGHT_4_TM=60.231
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_04974 Ps_contig_mira-and-tgicl-ass_franssen_35760
SEQUENCE=TAGGAATGGCTATCAAAATACCCCTAATTGACCATCTACCTAACYTATTCTTTTTTTTTCCTTTTCCATTTTCTAACCATTCTCAACTTCTCATCACAAGTTATCACCTTTTTTACTCAAACAATTTCCCATGTAAATAATAAACAATGATACAACAAAACGGTGGCAACATGTGATGAGAAGAATTGTAACATAACACAACTAACTAACTATTTACAAATATAAAGGAATCGCTATTTGCGGTGGCTTTATCGGCGGCGGTTTCACAACCGTCACAACAATATTTTCCGGCCGCGAAGGTTCACACGGACACGGCATCGCTATGAATCTTGGCACATCATCTCCCGGCATCATCACCGAATAGCTTTCTCCTCTGTTTTGCTTCTGTTCGGTGGAGTAAAGTTTAGATTTATTGGTTGAATCGATTTGAGGGTCTGAATCTAAGGAAGATTGACGGAGGGAACGGAGTTTATCCCAATGGTAGCAACAAGAGAGGATGCCACTCAAGCTAAAAATGATGATCAAGAGAAGTGCAGTGCCCAAGGGGAAACCGAGGGACGGTCGAGATACGTCCACGTGGGATGGTGAAAGATGATCCTGACTCTCCATTGTGTTTTTGTTGATGATGTTGCTTCACCAACACTCATGTGTTTGAGGAACGAATAACTTTTGAATGTGAATATCNNCTACTCTGCGTTGATACCA
TARGET=200,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04975 Ps_contig_mira-and-tgicl-ass_franssen_35771
SEQUENCE=AAGCAGTGGATCAACGCAGAGTAAGGGGGGGGNTTGGAGAATAATGCAGGTCTCAAACCATATGTGACCATTTTTCCATTGGGATGTTCCACAAGCCTTAGAGGATGAGTATGGCGGTTTTTTAAGCCGTCACATTGTAGATGATTTTAGGGACTATTTGCTTCAAGAAATTTGGTGATAGAGTGAAACGTTGGATTACTTTGAATGAACCAAGAAGTGTGAGTAAGAATGGCCATGCAAACGGAAGGTTTGCACCAGGGCGATGCTCAGACTGGTTGAAAATGAATTGCACAGGAGGTGATTCGGGGACAGAACCGTATTTGACTTCGCACTACCAACTTCTTGCTCATGCTGCTGCTGCAAAACTGTACAAGACCAAGTATCAGGCATCTCAAAAGGGTCTACTAGGGATTACCTTAAACTCTGATTGGTTTGTGCCTGTCTCTAAAGAGAAAGCAGATCGTGATGCGGCACAACGAGCCCTCGACTTCATGTTTGGATGGTAAAGATTAAACTTGAGTATTCCTTGCCCAACAAGAGAGATTATATTAAAAATTTGAAAATATGATCATTAGGGGGTGTTATAACTTATTCTACTCGGTAAAGAAAATTGTGGACCAACTACATA
TARGET=350,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0702
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=TGGTGATAGAGTGAAACGTTGG
PRIMER_RIGHT_SEQUENCE=AGGCACAAACCAATCAGAGTTT
PRIMER_LEFT=172,22
PRIMER_RIGHT=439,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=0.1606
PRIMER_LEFT_1_PENALTY=0.030276
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=TGGTGATAGAGTGAAACGTTGG
PRIMER_RIGHT_1_SEQUENCE=GTAGACCCTTTTGAGATGCCTG
PRIMER_LEFT_1=172,22
PRIMER_RIGHT_1=404,22
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.1871
PRIMER_LEFT_2_PENALTY=0.186309
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=CGTATTTGACTTCGCACTACCA
PRIMER_RIGHT_2_SEQUENCE=TTGGGCAAGGAATACTCAAGTT
PRIMER_LEFT_2=315,22
PRIMER_RIGHT_2=533,22
PRIMER_LEFT_2_TM=60.186
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.2262
PRIMER_LEFT_3_PENALTY=0.186309
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=CGTATTTGACTTCGCACTACCA
PRIMER_RIGHT_3_SEQUENCE=AGGCACAAACCAATCAGAGTTT
PRIMER_LEFT_3=315,22
PRIMER_RIGHT_3=439,22
PRIMER_LEFT_3_TM=60.186
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=0.3254
PRIMER_LEFT_4_PENALTY=0.186309
PRIMER_RIGHT_4_PENALTY=0.139129
PRIMER_LEFT_4_SEQUENCE=CGTATTTGACTTCGCACTACCA
PRIMER_RIGHT_4_SEQUENCE=TTACCATCCAAACATGAAGTCG
PRIMER_LEFT_4=315,22
PRIMER_RIGHT_4=505,22
PRIMER_LEFT_4_TM=60.186
PRIMER_RIGHT_4_TM=59.861
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_04976 Ps_contig_mira-and-tgicl-ass_franssen_35798
SEQUENCE=GGGAAGGATGCTCCATAATAAAGAGAGAGAGATGTTATCCCTTCTCTCTCTAGAACTTTTCTCCACAACCCTTTAGGTTTTGGCGGCCGCCTCTCACATTTATGGTGGTTTTCCTCTCCCGCACTTATAGTGGTTACCCCCTCCTCCATTGAAAGTACTATCCACTCCTTTCAACTTCACTCACATGCTCTCACCACAACAAACTCACTCTTCGATTACCCCCTTTCTCCACTTTAAACCACCACCAACCATCTCTTTTTATATCTATGGCGACCACCTGAGCCACCACCACCAACAAGAAATGGATAATTTGCAATGGTAGGTTTTCACATCTCAAGCTTAACAACGATGAAGGTACCTCTCCTCCGTGTAGCTGATCTTTCACCTCCAATGTCGACAACCAATCGTCTACCTCCAAAGCCACCATGGATACTCTACACCTTGCAACCTATGTCAGATTTGTTTAATCCTGCTTATGTTTATGTACTGTTTTCATCCATTAATCTTCGAAACATTCGATAAAAATGGATAATATTGAGAACAAAGATAGTTGAAACATGCAAACACAAGTAAAAGAAATCATCTGTAACTGGATTTGAAAAAGACCGAGGATTCTCTTTTCTATCTTGCCATTTATCGTGCTCACAAGAGATTGGTTGAATCAATGATAAGTTGTTGCTTTTGTTTATGGTGTGTTTATGTCTTATTAGCTTGTGAACATTTATTCTATGTGTATCGTCTTTTATGATTATGTAGGTTTTTGTTTAGATTTGTCTCTAGATTA
TARGET=22,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7632
PRIMER_LEFT_PENALTY=0.682355
PRIMER_RIGHT_PENALTY=0.080808
PRIMER_LEFT_SEQUENCE=GGGAAGGATGCTCCATAATAAA
PRIMER_RIGHT_SEQUENCE=GTTTGTTGTGGTGAGAGCATGT
PRIMER_LEFT=0,22
PRIMER_RIGHT=203,22
PRIMER_LEFT_TM=59.318
PRIMER_RIGHT_TM=60.081
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=0.9022
PRIMER_LEFT_1_PENALTY=0.682355
PRIMER_RIGHT_1_PENALTY=0.219884
PRIMER_LEFT_1_SEQUENCE=GGGAAGGATGCTCCATAATAAA
PRIMER_RIGHT_1_SEQUENCE=GCATGTGAGTGAAGTTGAAAGG
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=187,22
PRIMER_LEFT_1_TM=59.318
PRIMER_RIGHT_1_TM=59.780
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=188
PRIMER_PAIR_PENALTY_2=0.9105
PRIMER_LEFT_2_PENALTY=0.682355
PRIMER_RIGHT_2_PENALTY=0.228153
PRIMER_LEFT_2_SEQUENCE=GGGAAGGATGCTCCATAATAAA
PRIMER_RIGHT_2_SEQUENCE=GTTGTGGTGAGAGCATGTGAGT
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=199,22
PRIMER_LEFT_2_TM=59.318
PRIMER_RIGHT_2_TM=60.228
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=0.9259
PRIMER_LEFT_3_PENALTY=0.682355
PRIMER_RIGHT_3_PENALTY=0.243544
PRIMER_LEFT_3_SEQUENCE=GGGAAGGATGCTCCATAATAAA
PRIMER_RIGHT_3_SEQUENCE=GTTTAAAGTGGAGAAAGGGGGT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=59.318
PRIMER_RIGHT_3_TM=59.756
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=10.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=0.9301
PRIMER_LEFT_4_PENALTY=0.682355
PRIMER_RIGHT_4_PENALTY=0.247794
PRIMER_LEFT_4_SEQUENCE=GGGAAGGATGCTCCATAATAAA
PRIMER_RIGHT_4_SEQUENCE=CTATAAGTGCGGGAGAGGAAAA
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=129,22
PRIMER_LEFT_4_TM=59.318
PRIMER_RIGHT_4_TM=59.752
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_04977 Ps_contig_mira-and-tgicl-ass_franssen_35798
SEQUENCE=GGGAAGGATGCTCCATAATAAAGAGAGAGAGATGTTATCCCTTCTCTCTCTAGAACTTTTCTCCACAACCCTTTAGGTTTTGGCGGCCGCCTCTCACATTTATGGTGGTTTTCCTCTCCCGCACTTATAGTGGTTACCCCCTCCTCCATTGAAAGTACTATCCACTCCTTTCAACTTCACTCACATGCTCTCACCACAACAAACTCACTCTTCGATTACCCCCTTTCTCCACTTTAAACCACCACCAACCATCTCTTTTTATATCTATGGCGACCACCTGAGCCACCACCACCAACAAGAAATGGATAATTTGCAATGGTAGGTTTTCACATCTCAAGCTTAACAACGATGAAGGTACCTCTCCTCCGTGTAGCTGATCTTTCACCTCCAATGTCGACAACCAATCGTCTACCTCCAAAGCCACCATGGATACTCTACACCTTGCAACCTATGTCAGATTTGTTTAATCCTGCTTATGTTTATGTACTGTTTTCATCCATTAATCTTCGAAACATTCGATAAAAATGGATAATATTGAGAACAAAGATAGTTGAAACATGCAAACACAAGTAAAAGAAATCATCTGTAACTGGATTTGAAAAAGACCGAGGATTCTCTTTTCTATCTTGCCATTTATCGTGCTCACAAGAGATTGGTTGAATCAATGATAAGTTGTTGCTTTTGTTTATGGTGTGTTTATGTCTTATTAGCTTGTGAACATTTATTCTATGTGTATCGTCTTTTATGATTATGTAGGTTTTTGTTTAGATTTGTCTCTAGATTA
TARGET=283,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1189
PRIMER_LEFT_PENALTY=0.080808
PRIMER_RIGHT_PENALTY=0.038090
PRIMER_LEFT_SEQUENCE=ACATGCTCTCACCACAACAAAC
PRIMER_RIGHT_SEQUENCE=AGATCAGCTACACGGAGGAGAG
PRIMER_LEFT=182,22
PRIMER_RIGHT=379,22
PRIMER_LEFT_TM=60.081
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=0.1738
PRIMER_LEFT_1_PENALTY=0.080808
PRIMER_RIGHT_1_PENALTY=0.093026
PRIMER_LEFT_1_SEQUENCE=ACATGCTCTCACCACAACAAAC
PRIMER_RIGHT_1_SEQUENCE=AAAGATCAGCTACACGGAGGAG
PRIMER_LEFT_1=182,22
PRIMER_RIGHT_1=381,22
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=59.907
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.2223
PRIMER_LEFT_2_PENALTY=0.184188
PRIMER_RIGHT_2_PENALTY=0.038090
PRIMER_LEFT_2_SEQUENCE=GCTCTCACCACAACAAACTCAC
PRIMER_RIGHT_2_SEQUENCE=AGATCAGCTACACGGAGGAGAG
PRIMER_LEFT_2=186,22
PRIMER_RIGHT_2=379,22
PRIMER_LEFT_2_TM=59.816
PRIMER_RIGHT_2_TM=60.038
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.2533
PRIMER_LEFT_3_PENALTY=0.215254
PRIMER_RIGHT_3_PENALTY=0.038090
PRIMER_LEFT_3_SEQUENCE=CATGCTCTCACCACAACAAACT
PRIMER_RIGHT_3_SEQUENCE=AGATCAGCTACACGGAGGAGAG
PRIMER_LEFT_3=183,22
PRIMER_RIGHT_3=379,22
PRIMER_LEFT_3_TM=60.215
PRIMER_RIGHT_3_TM=60.038
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=197
PRIMER_PAIR_PENALTY_4=0.2580
PRIMER_LEFT_4_PENALTY=0.219884
PRIMER_RIGHT_4_PENALTY=0.038090
PRIMER_LEFT_4_SEQUENCE=CCTTTCAACTTCACTCACATGC
PRIMER_RIGHT_4_SEQUENCE=AGATCAGCTACACGGAGGAGAG
PRIMER_LEFT_4=166,22
PRIMER_RIGHT_4=379,22
PRIMER_LEFT_4_TM=59.780
PRIMER_RIGHT_4_TM=60.038
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_04978 Ps_contig_mira-and-tgicl-ass_franssen_35800
SEQUENCE=TAACACACTCAACAAGTCAATTTTATTATGTTATCTGTCACGAATACTATTAGTAGCTCATACGATGAAAAAGAAGAAACCCGAACTCTTTCCTATCATTCGTTGGCTTTCTAACTCAAGCTTCTGAAATGGGCTGCTATTTGGATCGTAGTAGAACCCGCTGATAGAGCCATCGCTGCACGAGAAACAAACATAGTAAAATCCAGCTATAGTTAACCCACAGTCAGTTCCGACATTCACGAAGTATTGCTCCTTCCATCTCATGAATATGTAGGGATAGTTGCTTAGGTCCATAGATTTGCCACCATCTACCTCTACTTGGCTCAATAGAGGGGAAAACGATGGAAATTTAGTCCAGTGCCTTATATCATCTTCTGGTGCTGCCTCCCATTTTCCGGTGAAGAAAGTATAATTTCTTGCATCAACAATCTCCCCTTCCCAGAATGTTACTACGGGTGTGTCTGCCATTGGAACATTAAGAGCTTCCATGGTGCCACATAGATATCCATGCTCAAGATCGCATCCTTGTAAACGGACATTTACCCTCCATGCTTCGTCTTTTTGTAAGCTTGAAACATTCTGAGTCCCAGAAAAGGCCTGTCCAGCACATAGAAGGGTACAAGATTGGAATGAACGACGCCTTGGAGTTACAGCAGAAACGTGAGAAGGGGTGGGAGTGTTCTCCAATACTACTCGCACAGGCATGTTGCAGAGGACACAACACTGATCGCACTCGTTTTCTGTTTTTGTTTTGAATCAAAGCCAGAAGAGAAGAGAAGAGAACAAACAAACGAATTTCCCCCGACGTACTCTGCGTTGATACCCACTGTCTT
TARGET=765,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1932
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.189596
PRIMER_LEFT_SEQUENCE=TACAAGATTGGAATGAACGACG
PRIMER_RIGHT_SEQUENCE=CAGTGGGTATCAACGCAGAGTA
PRIMER_LEFT=617,22
PRIMER_RIGHT=828,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.190
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.1000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.4326
PRIMER_LEFT_1_PENALTY=0.243031
PRIMER_RIGHT_1_PENALTY=0.189596
PRIMER_LEFT_1_SEQUENCE=AGCTTGAAACATTCTGAGTCCC
PRIMER_RIGHT_1_SEQUENCE=CAGTGGGTATCAACGCAGAGTA
PRIMER_LEFT_1=566,22
PRIMER_RIGHT_1=828,22
PRIMER_LEFT_1_TM=59.757
PRIMER_RIGHT_1_TM=60.190
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.4955
PRIMER_LEFT_2_PENALTY=0.305953
PRIMER_RIGHT_2_PENALTY=0.189596
PRIMER_LEFT_2_SEQUENCE=ATGTTGCAGAGGACACAACACT
PRIMER_RIGHT_2_SEQUENCE=CAGTGGGTATCAACGCAGAGTA
PRIMER_LEFT_2=703,22
PRIMER_RIGHT_2=828,22
PRIMER_LEFT_2_TM=59.694
PRIMER_RIGHT_2_TM=60.190
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.5283
PRIMER_LEFT_3_PENALTY=0.338748
PRIMER_RIGHT_3_PENALTY=0.189596
PRIMER_LEFT_3_SEQUENCE=TTGGAGTTACAGCAGAAACGTG
PRIMER_RIGHT_3_SEQUENCE=CAGTGGGTATCAACGCAGAGTA
PRIMER_LEFT_3=641,22
PRIMER_RIGHT_3=828,22
PRIMER_LEFT_3_TM=60.339
PRIMER_RIGHT_3_TM=60.190
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=0.5578
PRIMER_LEFT_4_PENALTY=0.368190
PRIMER_RIGHT_4_PENALTY=0.189596
PRIMER_LEFT_4_SEQUENCE=TCCAGCACATAGAAGGGTACAA
PRIMER_RIGHT_4_SEQUENCE=CAGTGGGTATCAACGCAGAGTA
PRIMER_LEFT_4=600,22
PRIMER_RIGHT_4=828,22
PRIMER_LEFT_4_TM=59.632
PRIMER_RIGHT_4_TM=60.190
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_04979 Ps_contig_mira-and-tgicl-ass_franssen_35801
SEQUENCE=TCAGCTCCACTGGCAAATATGGTGAATACATGTTACAAGACTGCAGGAAATGTGCTGCCTAACTTCATCGCAGTTAACTTTTACATGAGGAGTGATGGTGGCGGTGTTTTTGACATCGTTGATCGAATAAACGGCCACACATTGTGTGGATGCAGTACAGTCACTGCTTGCCAGGCGGGAGCACCTTTTGGATCTTGCAAGAACATTTCTGTACCAATTACAAGTCCAGTGACCAACACGCCTGGAAGCTTTAACGGTTACGTTCAGTTTTCAATTAAATCCGCTTCACCAGTCCATTCTCCAAATCGCTCGCTTTTCCTCTTGTTTTACTTCCTGCTTACCGCAGTTCTTTTGCAGTTTCGATGAGTTTTATCGTATATCATATGCAAACTAGGTCTGTCAAGATAAGTTTCTGGTATTGTCATATTTGCATATCATATGCGAGATAGTGCCGAGTCTCTCGAGCGAGGCTTCTAATATCTTACTATGTAGTATAATGGATTATATAGCCTCGATCTCGAAATGTCATGGAATGGTTTAGAGGTTAGAAAATTAGGCAACAAAATTTCAGAAAAGGAAAGGCTATAATCAAGAATCTGAGAAGTCAATCAAGAATATATATATCTAGAGCATTTCAGGTGTGTTGCATATTTGGCATCTTATCCGTCGTCGCATATGCATAACATTGCATGTGTAATTAATTGTCATGTCATATCACTAATAT
TARGET=615,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2070
PRIMER_LEFT_PENALTY=0.102418
PRIMER_RIGHT_PENALTY=0.104555
PRIMER_LEFT_SEQUENCE=TATCATATGCGAGATAGTGCCG
PRIMER_RIGHT_SEQUENCE=AGATGCCAAATATGCAACACAC
PRIMER_LEFT=432,22
PRIMER_RIGHT=659,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=59.895
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=0.2070
PRIMER_LEFT_1_PENALTY=0.102418
PRIMER_RIGHT_1_PENALTY=0.104555
PRIMER_LEFT_1_SEQUENCE=TATCATATGCGAGATAGTGCCG
PRIMER_RIGHT_1_SEQUENCE=ATATGCAACACACCTGAAATGC
PRIMER_LEFT_1=432,22
PRIMER_RIGHT_1=650,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=59.895
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=0.2972
PRIMER_LEFT_2_PENALTY=0.102418
PRIMER_RIGHT_2_PENALTY=0.194801
PRIMER_LEFT_2_SEQUENCE=TATCATATGCGAGATAGTGCCG
PRIMER_RIGHT_2_SEQUENCE=ACGGATAAGATGCCAAATATGC
PRIMER_LEFT_2=432,22
PRIMER_RIGHT_2=666,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=60.195
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.7000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=235
PRIMER_PAIR_PENALTY_3=0.3611
PRIMER_LEFT_3_PENALTY=0.256503
PRIMER_RIGHT_3_PENALTY=0.104555
PRIMER_LEFT_3_SEQUENCE=CTCTCGAGCGAGGCTTCTAATA
PRIMER_RIGHT_3_SEQUENCE=AGATGCCAAATATGCAACACAC
PRIMER_LEFT_3=457,22
PRIMER_RIGHT_3=659,22
PRIMER_LEFT_3_TM=60.257
PRIMER_RIGHT_3_TM=59.895
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.3611
PRIMER_LEFT_4_PENALTY=0.256503
PRIMER_RIGHT_4_PENALTY=0.104555
PRIMER_LEFT_4_SEQUENCE=CTCTCGAGCGAGGCTTCTAATA
PRIMER_RIGHT_4_SEQUENCE=ATATGCAACACACCTGAAATGC
PRIMER_LEFT_4=457,22
PRIMER_RIGHT_4=650,22
PRIMER_LEFT_4_TM=60.257
PRIMER_RIGHT_4_TM=59.895
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_04980 Ps_contig_mira-and-tgicl-ass_franssen_35814
SEQUENCE=TGATAATATAAAATCAAAAATAATTTATTTTGAGAAAAATAATACATGAGTACCGTCAGTTTTCTCATACTTTCCTGGCCCATTTTTGCGTACCTTTGTTGATTTTCATTATTTCAGCCCAAACATTAAGATCTTTCTAACCAACGTGAGACTAACCAACACTTACACTTGAATTGCAACATTGTTTCAATTTAAGGGAGCTTTGCAAAAGGAACAAAATGATCCTACCAAATTCTAATCAATAAGAATATTGTAATTTACAGGGGGGCATGTTGGGTCTACCATTTTTCTTGATTCTCTTTCATCATCATTTTTTATATTTATTGTTTACTCAGATATCAGTTCCCCCAATCCAGTCATATGGACCTGCCCTCGTTTGCAAATGCGCATCGGCTACCTCTACTTTCTACCCTCTACTGGGTCTAGTCGTCGATGTCTTTGACAGCATCCTTTGCAAGTTTCGTGACATATGTAGCAGCTAAGGCAGTGACAAATAATCCAAGCCCAAGGGTCAGTAGCTGATTGTTTCCTCCAAGTGCACTAAGTTCAGATTCTTCTTGGATTATTGCTCTTCCAAAAGCACCTGCACTAACATAAGCCCATGTTCCTGGAAGCATCCCCAACCAACTTCCCAGTACATACGGAAGAAACTTAACAGATGTCAATCCATATAAATAATTCCCCAAAGAAAATGGCAGCAACGGGCTCAAGCGAAGCAGGGTCACAACCTTGAAGCCATTTTCTCCGATTGCTTTGTCAATAGCAAGAAACTTCTTGTTTCCTTCCACTAGTTTTAGAATGCGCTCGCGAGCAAAATATCTTGCAATTAGAAAAGCAACACTAGCCGCCACTGTTCCACTGATAGAGACTATGATAGTGCCAGTAAGAGATCCAAAAAGAAGTCCAGCTGACATGGTTAAGGGAATGGCAGGAATAGCAAGGATCTCCAATCCAGCATAAACAGCTACAAATAATGCATATCCAGCTGAACCATAACCTTCAATGAAGCCAGAGAACTGATTCAAGAAAGAATTAATAGGTTCTCTGTAGAAATAACCAACGGAAGCAAAACCCCCAACAAAACCAACAAGCAAAACACCAGCCAAAATTGTACCCTTCAAAGCAGTACTACCATCATCATCATCATCATCATCCTTCCCCCGTTTGTCAGATTCAGATTCTCTTCTTCTGTTCTTCCCAACATCCCTCTTATCATCTTCCCCTTTGGAGTTAAACAAACGCTTTAACCGCGACGGACTAGTATTACTGATCTTAGTAGGTTGTTTTTTGGTTTGTTTGAGAGAAGAATGCGGTGTTAGAAAGATAGAAGACGAAATCGGTGAAGAAGAGAATAAGCGAAAACGGAAGCTGGAATTGGAATTGTTGATGTGAAGAGAAGAACAACAAGAAGAAGATAGACATGGTGATGGTAATGAGAGAGTGCGCATGATAAGAATTCAAACTAATTCTCGTGAGAAGTAAGAATTAGTTACGAGAAGAAGAACGGAATTGATAATCGATTATTCATGAAAGTGCTTTATTTTTTTTCTTCTATCTATCTTAACTTTCATTCTTGCGGGAATGTGATGTGTGTATCTA
TARGET=1133,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0331
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=0.026163
PRIMER_LEFT_SEQUENCE=TTCAATGAAGCCAGAGAACTGA
PRIMER_RIGHT_SEQUENCE=TGTTTAACTCCAAAGGGGAAGA
PRIMER_LEFT=998,22
PRIMER_RIGHT=1236,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.974
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.0331
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=0.026163
PRIMER_LEFT_1_SEQUENCE=TGAAGCCAGAGAACTGATTCAA
PRIMER_RIGHT_1_SEQUENCE=TGTTTAACTCCAAAGGGGAAGA
PRIMER_LEFT_1=1003,22
PRIMER_RIGHT_1=1236,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=59.974
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.0501
PRIMER_LEFT_2_PENALTY=0.006945
PRIMER_RIGHT_2_PENALTY=0.043121
PRIMER_LEFT_2_SEQUENCE=TTCAATGAAGCCAGAGAACTGA
PRIMER_RIGHT_2_SEQUENCE=ATACTAGTCCGTCGCGGTTAAA
PRIMER_LEFT_2=998,22
PRIMER_RIGHT_2=1263,22
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=60.043
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.0501
PRIMER_LEFT_3_PENALTY=0.006945
PRIMER_RIGHT_3_PENALTY=0.043121
PRIMER_LEFT_3_SEQUENCE=TGAAGCCAGAGAACTGATTCAA
PRIMER_RIGHT_3_SEQUENCE=ATACTAGTCCGTCGCGGTTAAA
PRIMER_LEFT_3=1003,22
PRIMER_RIGHT_3=1263,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=60.043
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.0681
PRIMER_LEFT_4_PENALTY=0.006945
PRIMER_RIGHT_4_PENALTY=0.061184
PRIMER_LEFT_4_SEQUENCE=TTCAATGAAGCCAGAGAACTGA
PRIMER_RIGHT_4_SEQUENCE=TTAACTCCAAAGGGGAAGATGA
PRIMER_LEFT_4=998,22
PRIMER_RIGHT_4=1233,22
PRIMER_LEFT_4_TM=59.993
PRIMER_RIGHT_4_TM=59.939
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_04981 Ps_contig_mira-and-tgicl-ass_franssen_35830
SEQUENCE=AATTGTTCTACATGATTACACTGTCAAATTTTTATAGGTTGAGAGAGAAAAAGAGAAGGTGGAGAAGAGAAGAAAAATAGAGAGAGAGAGATTGGGTTGTGTTTTGTTGAGTTTGATGGTGAAGGGGTGAARRAAAAAACAAGATAGACACATGGAATTATTAATGGGTTGTGAATTAAGGTGGGTTTGTTTTTTTGTTAAGTAATTGACTTAAAACATTTG
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04982 Ps_contig_mira-and-tgicl-ass_franssen_35833
SEQUENCE=CCCCGCCCCGCCGCAGTGTGGCAAAGTAACCAACCAACCTCCCTCTGCTGCTGTACTGTGGCGGCCGTTGAATAGAACAATGGGTAGATTTGTCCGTCTAGCTCGGAATATTTTCTCTTCCCCCGCCTTATTTCCCCATCCTTTCAAACACCTTCGTTTCGCTCCTCAATCACCGTTCACTGCTGCTAATTATTTCCACTCAGTTTCAGTTATTGTTCCCAACACTGAGACCCAACAAACAAATTCCGCAAACTCTACAAAACCGCCATCACTGTTAAGAGTAAGGAAGAAGGCTCAATCTCGCCGCAACTCCGCATTCACACATACTAATAATAATACAGCCGTCGAGCTTGCATTCAATTCTGTTGTCAAAATCTTCACCGTTTCATGCAGTCCCAACTACTTACTTCCTTGGCAGAACAAATCCCAGCGCGAATCTATGGGCTCGGGGTTTGTAATTCTGGGCAGAAAGATTCTTACCAATGCTCATGTTGTAGCTGATCATTCATTTGTACTTGTAAGAAAACATGGATCTCCAAATAAGTATAGAGCACAAGTTAAAGCTGTTGGTCATGAATGTGACTTAGCTTTATTAATTGTTGACTCTGATGAATTTTGGGATGGAATGATTCCTTTGGAGTTTGGAGATATTCCTTTTCTGCAACAAGCTGTTGCTGTTGTAGGATACCCTCAAGGAGGTGATACCATTTCAGTCACTAAAGGGGTTGTT
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04983 Ps_contig_mira-and-tgicl-ass_franssen_35839
SEQUENCE=ATTAGAGAGTATTTAATACATGGCTTGTTTTGTTTGAGAGATTTGAGGCCATTGAGATGGGAAGAAGATCATCATCATCATAAGCATGATAGTATGATGACATTGTGGAGAAGTCAACCTCGTGATCTTTATCGTAGGTCACTTCCGGGACTTGTGTTGGCTATTTAGAACACATGACAATGTTTGTGTCTTGTGTTTTATTTGGTTGTTTTAATGTAATGTGTTGTGTACATCCTTTTCATGTCTTGTGGTTAATGGTTATGCGAAGTTATCACCAATTTGGAAGGAAAAGAATGAATAAGAGTTCAAAGATATCATGTTTGTGTACTTTGTTCCTCTCTTCCCCTTTCATTTGCTTGTTTTTCTTTTCTTTTAGAATGACATGAAAAATGTGTCCAAACCACTTTACTCAACGTTTATGTGTAGTGAAGGTATCCCTTCGAATCAAATTCCTCGCAATTGTTGTTTTTGCCGCATTTATGCAGTTAAGGGTTAAYT
TARGET=68,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04984 Ps_contig_mira-and-tgicl-ass_franssen_35840
SEQUENCE=CTCAGAACAGTTTCCAGACACTGCTGGCACCATTGTTTCGATTCATGTTGATTCTGATGTGRTGTCGTTAGATGGAAGACAGTGTTTGGAAACAGAAGCTGAAGTTAAAGAAGATGGGAAGCTTCATGTTACAGTGAGAAAATCAAATGCTTCAAGATCGGATATATACTCGAGAAGATCTCATAATTCTCATTCTCATGCAAATACAACACCTCGCGCTTCAAATCTGACAAATGCTGAGATATACTCTTTGCAATCTTCAAGGAATCCAACTCCAAGAGGGTCAAGTTTCAATCACACAGATTTCTATTCAATGATGGGTCGTAATTCGAACTTTGGTGCTTCTGATTTGAAGGTAACAACGCCGAGAAGTTCTAACTATGATCTTTTCAACAACAGGGAACTATCCTGCACCGAATCCTGGAATGTTCTCTCCCAAGAATGTTGCTGCTAAGAAAGCTAATAATAATAATAATAATAGTAATAGTAATGGTCAAGTAGCAGTGAAAACAGATGATCTTCATATGTTTGTTTGGAGTTCAAGCGGTTCACCGGTTTCTGATGTGTTCGGTTCTCATCTTGAATATGGAGGAACAGGAACTGGAAATGGGAATGGAAATGGAGTTCAAGTTCATCATGATCAGAAACAAGAACAAGTCAAGTTGAATCTCTCTCCCAGAAAAGTGGAGGGTGAAGAAGAGTACATAGATGAGTCTAGGAATGAAGAGACTGGAAAGGGAAACT
TARGET=461,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04985 Ps_contig_mira-and-tgicl-ass_franssen_35841
SEQUENCE=GTCGTATTTTCTCTTGATGTTGTAAATTACTTCTAAGCAACTTTTTGGGATCAGAACAACCATCTCGAAAGAATTCTGCAATTGCTATATTATGAAGGACCTTCGGATCGTTAGGTTTCTTTTGCAAGAGCTGATTCATGACCTCGACGCATTCAACGAACTTGCCAGATTGATAATGCATAGCAGCGTCTTTGGCGAGAGCGAAAAGGCCGTCGTCAACTTCGGCGGCGGCGGAAGGGGCATCGCGGTTGTTGGTTGAGGGAGAAGAAGAAGGTAACGAATCACGAGATTCCATAGCGAGATCGAGTAATCAGAATACACAAATCTTGGTACGAAAGGGTTTGGGGAAGAGAGAGAAAGAGCCCTAAAATTGAGTGAGGAAAAGTGAAGTGAAATGAATCTAAATCTGAATTTACATTTAAAATTGAAGTTAAAAAGAAAACATGGTTGAAGAGAAAAACCCTTGCTCCTTGTTTTTGCTAGCTTAGATAGAGAAGAAGAAATGAGTGAGAGTCCCCCCGGACTCTGCGTTGATACCA
TARGET=223,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2073
PRIMER_LEFT_PENALTY=0.098039
PRIMER_RIGHT_PENALTY=0.109273
PRIMER_LEFT_SEQUENCE=TGATAATGCATAGCAGCGTCTT
PRIMER_RIGHT_SEQUENCE=ACCTTCTTCTTCTCCCTCAACC
PRIMER_LEFT=170,22
PRIMER_RIGHT=274,22
PRIMER_LEFT_TM=59.902
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=105
PRIMER_PAIR_PENALTY_1=0.2073
PRIMER_LEFT_1_PENALTY=0.098039
PRIMER_RIGHT_1_PENALTY=0.109273
PRIMER_LEFT_1_SEQUENCE=TTGATAATGCATAGCAGCGTCT
PRIMER_RIGHT_1_SEQUENCE=ACCTTCTTCTTCTCCCTCAACC
PRIMER_LEFT_1=169,22
PRIMER_RIGHT_1=274,22
PRIMER_LEFT_1_TM=59.902
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=0.2110
PRIMER_LEFT_2_PENALTY=0.101680
PRIMER_RIGHT_2_PENALTY=0.109273
PRIMER_LEFT_2_SEQUENCE=GGTTTCTTTTGCAAGAGCTGAT
PRIMER_RIGHT_2_SEQUENCE=ACCTTCTTCTTCTCCCTCAACC
PRIMER_LEFT_2=113,22
PRIMER_RIGHT_2=274,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=0.2266
PRIMER_LEFT_3_PENALTY=0.098039
PRIMER_RIGHT_3_PENALTY=0.128591
PRIMER_LEFT_3_SEQUENCE=TGATAATGCATAGCAGCGTCTT
PRIMER_RIGHT_3_SEQUENCE=GAGCAAGGGTTTTTCTCTTCAA
PRIMER_LEFT_3=170,22
PRIMER_RIGHT_3=468,22
PRIMER_LEFT_3_TM=59.902
PRIMER_RIGHT_3_TM=59.871
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.2266
PRIMER_LEFT_4_PENALTY=0.098039
PRIMER_RIGHT_4_PENALTY=0.128591
PRIMER_LEFT_4_SEQUENCE=TTGATAATGCATAGCAGCGTCT
PRIMER_RIGHT_4_SEQUENCE=GAGCAAGGGTTTTTCTCTTCAA
PRIMER_LEFT_4=169,22
PRIMER_RIGHT_4=468,22
PRIMER_LEFT_4_TM=59.902
PRIMER_RIGHT_4_TM=59.871
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=300
=
PRIMER_SEQUENCE_ID=SSR_04986 Ps_contig_mira-and-tgicl-ass_franssen_35871
SEQUENCE=AATATATGGGATAGACTGAGGAAGTANAGATGATCTGATAACTCCGTTATTAAAGTACATAATAGTTCTGCAATGGCATCATTCTCTGTAAACGTCACCGCTTCACCTTCTCCACTCATTCTTCAACGACGTCCTTTCTCCAACTTTTCATCCTCTACTCATCATCAACTACAAGATTCACATCTGCAAAATAAAAAATTTGGGGCTTTCTCATTACTGGGTTTCGCACCATTTTCTTTCAATTTGAGGAGGAGGAGAACTTTGATGTGTGCAGCCAATCAAGATTCAATGGAAGTAGTGAAAGAAACAGGCAAAACGAATGGCCTGATCGGTGTATTGGGCGAAGTTGATTCTCGTCAAGTACATGACAAAAGTACTGAAGCTTGGTCGCAATTTGCCAAAAGAATATCAGGTGAGTGGGATGGATTTGGAGCAGACTTCAGTAATCAAGGGAGCACAATCGAACTTCCGGAGTATGTGGTGCCTGAAGCATATCGAGAGTGGGAGGTCAAAGTATTTGATTGGCAGACTCAATGCCCCACTCTTGCTAACCCAGAGGATCATGTTCTCGAGTACAGGTCCATTCAGTTACTTCCTACAGTTGGATGTGAGGCTGATGCTGCAACGGTATACAGTTCGGATGAGAGAAAAGTGGGTGGCGAAAACAGCGGAGTTACCACTTTTGCTTACCAATCGAGTGGCTCTTATGTTGCTGTCTGGCAAAAGAAGGACGACGTAACAGAGTTGGAATACTGCTTGATCAATCCTCAAGATTTTGAATCTCGGGTTAGGATTATTCAGCGTATCCGTGTATCAAATAATACAGAGATTAAGCTACAGGGTGTAAGAGTATTTCGTGAGCAGTGGTATGGACCATTCAGAAACGGCGATCAACTAGGAGGTTGTGCCATTCGCGATTCCGCTTTTGCTTCTACAGCACCTATGATTGCTTCAGATATTGCTGGTATCTGGCAGGGTTCTAAAGCTATCACCACTTTCAGCGCTACAAACACCGAGATTTTTCGAGAGCTTTTAGACGACGGACTGCAGAAGTCTGTTAGAGATGGAGAAAACAATGTATTACTTCCTAAGCAGCTGTGGTGTTCGCTTAAACAAAACAAGGACGGCGAAACTATAAGCGAAGCGGGATGGCTTTTAGATCAAGGGCAGGCAATCACATCAAGTTGCTTATTCTCAAGCACTGCGAAGCTGAAGGAAATATCAATAGCATTAGAAACTAGAGCCTCGGAATAAGTAGAATAGCCTTGGTGGCAATCCATTCTGATCCAAATTTGTTTACATCTCTAAACGCATCACAACCAAGGTGAAATTCATCTATAAATATGATAAATTTGTCGCAAAAATYGGCATTATTTAAATGACTCATTTTAGTTTGTGTATATAAGCTCTATTAAGTTTTTATTTCTTCTGGTTCATGGTTAG
TARGET=246,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04987 Ps_contig_mira-and-tgicl-ass_franssen_35873
SEQUENCE=CTATTTGACACCACTCCTACCTGTAACCGTCATTCTTGTTTCCAAATCATTTTCATGGAAGCTTCCATCAAGTTTCAAGAACCATACCAAAGCCTGCTACAGCAGATTCCTCTGGCGCGCGCGCACACACACACCAAATAACCCTATCCGCAGCCAAGCCTAGTTCATTTCCAAAACCACTAACCTTCGGCTACACAAACTCAAACCAACTCAGACTACAAATTTTATTGAACCTTTATATACATCTATAGCATTAACTAACCCAGTTTCATCGCACACACTTATATACAGGATTACAACGCTCCTTGTATTTCACTCATCGTTAATGTTAAGATTGAGAATCCGACGCAACGTCTTAGTAGCAGAATCGGCATTAACAAAAAGATCCCTGGTAAAATCCAAACGCTCCCTAGTCGGAGAAGAAGGCGCAGACTTAGCCATGACACGCAACTCAATCTCCGGAGACGAACCAGGAAGATCAAGCGACACAGTCCTTTTAGGCCTCACAGAAAACTTAGGAATCGGCAACGAACTAGGTGGTGGCGAATTGGAAGTAAGTAGGTCCAGCCCATAACTCAGATAACAAAATGCTTCCATCAATTTCATCAAAAAACTCTTTCATTTCTAAAACCTTTTCCATTAATTGGAATTGGAGTACTCTTAGGAGTAGTTTTCCNAATTAGGCTTTCATCAAAG
TARGET=115,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0616
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.060824
PRIMER_LEFT_SEQUENCE=TTCAAGAACCATACCAAAGCCT
PRIMER_RIGHT_SEQUENCE=ACAAGGAGCGTTGTAATCCTGT
PRIMER_LEFT=73,22
PRIMER_RIGHT=308,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.0829
PRIMER_LEFT_1_PENALTY=0.027715
PRIMER_RIGHT_1_PENALTY=0.055230
PRIMER_LEFT_1_SEQUENCE=GAACCATACCAAAGCCTGCTAC
PRIMER_RIGHT_1_SEQUENCE=TGTAGCCGAAGGTTAGTGGTTT
PRIMER_LEFT_1=78,22
PRIMER_RIGHT_1=194,22
PRIMER_LEFT_1_TM=60.028
PRIMER_RIGHT_1_TM=60.055
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.0885
PRIMER_LEFT_2_PENALTY=0.027715
PRIMER_RIGHT_2_PENALTY=0.060824
PRIMER_LEFT_2_SEQUENCE=GAACCATACCAAAGCCTGCTAC
PRIMER_RIGHT_2_SEQUENCE=ACAAGGAGCGTTGTAATCCTGT
PRIMER_LEFT_2=78,22
PRIMER_RIGHT_2=308,22
PRIMER_LEFT_2_TM=60.028
PRIMER_RIGHT_2_TM=60.061
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.1030
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.102276
PRIMER_LEFT_3_SEQUENCE=TTCAAGAACCATACCAAAGCCT
PRIMER_RIGHT_3_SEQUENCE=GCGTCGGATTCTCAATCTTAAC
PRIMER_LEFT_3=73,22
PRIMER_RIGHT_3=348,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.1300
PRIMER_LEFT_4_PENALTY=0.027715
PRIMER_RIGHT_4_PENALTY=0.102276
PRIMER_LEFT_4_SEQUENCE=GAACCATACCAAAGCCTGCTAC
PRIMER_RIGHT_4_SEQUENCE=GCGTCGGATTCTCAATCTTAAC
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=348,22
PRIMER_LEFT_4_TM=60.028
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_04988 Ps_contig_mira-and-tgicl-ass_franssen_35873
SEQUENCE=CTATTTGACACCACTCCTACCTGTAACCGTCATTCTTGTTTCCAAATCATTTTCATGGAAGCTTCCATCAAGTTTCAAGAACCATACCAAAGCCTGCTACAGCAGATTCCTCTGGCGCGCGCGCACACACACACCAAATAACCCTATCCGCAGCCAAGCCTAGTTCATTTCCAAAACCACTAACCTTCGGCTACACAAACTCAAACCAACTCAGACTACAAATTTTATTGAACCTTTATATACATCTATAGCATTAACTAACCCAGTTTCATCGCACACACTTATATACAGGATTACAACGCTCCTTGTATTTCACTCATCGTTAATGTTAAGATTGAGAATCCGACGCAACGTCTTAGTAGCAGAATCGGCATTAACAAAAAGATCCCTGGTAAAATCCAAACGCTCCCTAGTCGGAGAAGAAGGCGCAGACTTAGCCATGACACGCAACTCAATCTCCGGAGACGAACCAGGAAGATCAAGCGACACAGTCCTTTTAGGCCTCACAGAAAACTTAGGAATCGGCAACGAACTAGGTGGTGGCGAATTGGAAGTAAGTAGGTCCAGCCCATAACTCAGATAACAAAATGCTTCCATCAATTTCATCAAAAAACTCTTTCATTTCTAAAACCTTTTCCATTAATTGGAATTGGAGTACTCTTAGGAGTAGTTTTCCNAATTAGGCTTTCATCAAAG
TARGET=125,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0616
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.060824
PRIMER_LEFT_SEQUENCE=TTCAAGAACCATACCAAAGCCT
PRIMER_RIGHT_SEQUENCE=ACAAGGAGCGTTGTAATCCTGT
PRIMER_LEFT=73,22
PRIMER_RIGHT=308,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.0829
PRIMER_LEFT_1_PENALTY=0.027715
PRIMER_RIGHT_1_PENALTY=0.055230
PRIMER_LEFT_1_SEQUENCE=GAACCATACCAAAGCCTGCTAC
PRIMER_RIGHT_1_SEQUENCE=TGTAGCCGAAGGTTAGTGGTTT
PRIMER_LEFT_1=78,22
PRIMER_RIGHT_1=194,22
PRIMER_LEFT_1_TM=60.028
PRIMER_RIGHT_1_TM=60.055
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.0885
PRIMER_LEFT_2_PENALTY=0.027715
PRIMER_RIGHT_2_PENALTY=0.060824
PRIMER_LEFT_2_SEQUENCE=GAACCATACCAAAGCCTGCTAC
PRIMER_RIGHT_2_SEQUENCE=ACAAGGAGCGTTGTAATCCTGT
PRIMER_LEFT_2=78,22
PRIMER_RIGHT_2=308,22
PRIMER_LEFT_2_TM=60.028
PRIMER_RIGHT_2_TM=60.061
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.1030
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.102276
PRIMER_LEFT_3_SEQUENCE=TTCAAGAACCATACCAAAGCCT
PRIMER_RIGHT_3_SEQUENCE=GCGTCGGATTCTCAATCTTAAC
PRIMER_LEFT_3=73,22
PRIMER_RIGHT_3=348,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.1300
PRIMER_LEFT_4_PENALTY=0.027715
PRIMER_RIGHT_4_PENALTY=0.102276
PRIMER_LEFT_4_SEQUENCE=GAACCATACCAAAGCCTGCTAC
PRIMER_RIGHT_4_SEQUENCE=GCGTCGGATTCTCAATCTTAAC
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=348,22
PRIMER_LEFT_4_TM=60.028
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_04989 Ps_contig_mira-and-tgicl-ass_franssen_35878
SEQUENCE=TGGTTGATGATGAGAAGGAAAATGGGAAACTTTAATTGGGGGAAAGATTGGTGGTGGAGACAAGATGGGAGTGGTGAATTGTGTAGTAAAGATTATGTTAAGGAATGGATTGGGAGTCAGATTTGTCCATCGAATGCTGATTGGGATGATGACGGTATCGCGAATACTAAGRACATGAACAGCATTCAGGGGAAATCTGAGTTGGAAAATTCAAGTCCAATAGATAAAGGTAATAATGATGCAATTGTAAATGGAACTCGGTTACATGTTTCTGCGACGGAAGATACTGATAATGGAGTTGTTGATATGAAAGAGGTGAAAGGAAAGAAAAACCAGAAGAAGAAGAAGATGAAGAAGAATAGGAAGATGCAAGAATGGTGGAAAGAAGAACATCTTGATGAGTCAAGCAAGAAGAAGAGAAACAAGCTGAGAAGTCTTGAAACGAAATGGAAGAAAGGGTTGAAATTGAAAGTTCCGCGATTTGGCTTAGGTAGAAGGTTCTATCATTGTGAAAGGGATAAGAACTATGG
TARGET=335,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04990 Ps_contig_mira-and-tgicl-ass_franssen_35887
SEQUENCE=TACCCTTCCTACCACCGGGTTCGGCCTTCGCCGATCTGCTCTCTCCCCAACCCCTTGAAATCAAACTCACAACCGTGCTGCTCCGGGTAACGGTGGGTCCCACACAGCATCATCCCGCACTTGCACTTAAACCCGGTGAGCCCCACGCGCTTCTTACACGTCATGCACCGATTCTGCTGCAGCGGTTTCGCCTTCGCCTCTTCAACAACGCTAGGCAAATCAACCGGCTCCGGCGACGAAGCTGACGGTTCAGAAACCAACGCCGCCTTCGCCGGCACCGCGATTGATTGATTGAGAACAAGCTTCGCCGATGAAGCTTGTTGTTCCTTAACCTGCAAATCTCGATAGCACCTTGAACAAAGATTCTCCGTGGCGGTGCTGCCAAAGAATCCACAATTGTTGACGCATAATCGCGGCGCTTGGCATCTGTGTTCTTCCGCCATCGAATCCGAATCGTTGAATTGATGAGATTTTTCAAATCTGTAAAAGCCGAAAAGGAGATATGTAAAAAGATTACCGTTACGGAGAATTATTATTAGAGAGGTTATGTAGAAAACATGAAAGAGAATCTGGAAAAGTAAAAGGAATTGAAGGAAAGGGTTAAAGAAAAGAATATTCAATCAATAGAGGAAGAAATTTTCAGAAACGTGTTTTGTTTTGTTTGGTACCAAACGGAGCACAGAGCCGCCCCNCGA
TARGET=282,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4466
PRIMER_LEFT_PENALTY=0.351175
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=CTTGAAATCAAACTCACAACCG
PRIMER_RIGHT_SEQUENCE=ATCGAGATTTGCAGGTTAAGGA
PRIMER_LEFT=53,22
PRIMER_RIGHT=345,22
PRIMER_LEFT_TM=59.649
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=293
PRIMER_PAIR_PENALTY_1=0.4766
PRIMER_LEFT_1_PENALTY=0.351175
PRIMER_RIGHT_1_PENALTY=0.125411
PRIMER_LEFT_1_SEQUENCE=CTTGAAATCAAACTCACAACCG
PRIMER_RIGHT_1_SEQUENCE=TGCTATCGAGATTTGCAGGTTA
PRIMER_LEFT_1=53,22
PRIMER_RIGHT_1=349,22
PRIMER_LEFT_1_TM=59.649
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.4803
PRIMER_LEFT_2_PENALTY=0.351175
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=CTTGAAATCAAACTCACAACCG
PRIMER_RIGHT_2_SEQUENCE=CGAGATTTGCAGGTTAAGGAAC
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=343,22
PRIMER_LEFT_2_TM=59.649
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.7583
PRIMER_LEFT_3_PENALTY=0.629225
PRIMER_RIGHT_3_PENALTY=0.129103
PRIMER_LEFT_3_SEQUENCE=AACCCCTTGAAATCAAACTCAC
PRIMER_RIGHT_3_SEQUENCE=CGAGATTTGCAGGTTAAGGAAC
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=343,22
PRIMER_LEFT_3_TM=59.371
PRIMER_RIGHT_3_TM=60.129
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.8486
PRIMER_LEFT_4_PENALTY=0.753123
PRIMER_RIGHT_4_PENALTY=0.095445
PRIMER_LEFT_4_SEQUENCE=ACCCCTTGAAATCAAACTCACA
PRIMER_RIGHT_4_SEQUENCE=ATCGAGATTTGCAGGTTAAGGA
PRIMER_LEFT_4=49,22
PRIMER_RIGHT_4=345,22
PRIMER_LEFT_4_TM=60.753
PRIMER_RIGHT_4_TM=60.095
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_04991 Ps_contig_mira-and-tgicl-ass_franssen_35887
SEQUENCE=TACCCTTCCTACCACCGGGTTCGGCCTTCGCCGATCTGCTCTCTCCCCAACCCCTTGAAATCAAACTCACAACCGTGCTGCTCCGGGTAACGGTGGGTCCCACACAGCATCATCCCGCACTTGCACTTAAACCCGGTGAGCCCCACGCGCTTCTTACACGTCATGCACCGATTCTGCTGCAGCGGTTTCGCCTTCGCCTCTTCAACAACGCTAGGCAAATCAACCGGCTCCGGCGACGAAGCTGACGGTTCAGAAACCAACGCCGCCTTCGCCGGCACCGCGATTGATTGATTGAGAACAAGCTTCGCCGATGAAGCTTGTTGTTCCTTAACCTGCAAATCTCGATAGCACCTTGAACAAAGATTCTCCGTGGCGGTGCTGCCAAAGAATCCACAATTGTTGACGCATAATCGCGGCGCTTGGCATCTGTGTTCTTCCGCCATCGAATCCGAATCGTTGAATTGATGAGATTTTTCAAATCTGTAAAAGCCGAAAAGGAGATATGTAAAAAGATTACCGTTACGGAGAATTATTATTAGAGAGGTTATGTAGAAAACATGAAAGAGAATCTGGAAAAGTAAAAGGAATTGAAGGAAAGGGTTAAAGAAAAGAATATTCAATCAATAGAGGAAGAAATTTTCAGAAACGTGTTTTGTTTTGTTTGGTACCAAACGGAGCACAGAGCCGCCCCNCGA
TARGET=649,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.6260
PRIMER_LEFT_PENALTY=0.251214
PRIMER_RIGHT_PENALTY=3.374827
PRIMER_LEFT_SEQUENCE=CCACAATTGTTGACGCATAATC
PRIMER_RIGHT_SEQUENCE=GCTCTGTGCTCCGTTTGGTA
PRIMER_LEFT=390,22
PRIMER_RIGHT=684,20
PRIMER_LEFT_TM=60.251
PRIMER_RIGHT_TM=61.375
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=3.7307
PRIMER_LEFT_1_PENALTY=0.355872
PRIMER_RIGHT_1_PENALTY=3.374827
PRIMER_LEFT_1_SEQUENCE=TCTGTAAAAGCCGAAAAGGAGA
PRIMER_RIGHT_1_SEQUENCE=GCTCTGTGCTCCGTTTGGTA
PRIMER_LEFT_1=479,22
PRIMER_RIGHT_1=684,20
PRIMER_LEFT_1_TM=60.356
PRIMER_RIGHT_1_TM=61.375
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=4.4238
PRIMER_LEFT_2_PENALTY=1.048930
PRIMER_RIGHT_2_PENALTY=3.374827
PRIMER_LEFT_2_SEQUENCE=CTGTAAAAGCCGAAAAGGAGAT
PRIMER_RIGHT_2_SEQUENCE=GCTCTGTGCTCCGTTTGGTA
PRIMER_LEFT_2=480,22
PRIMER_RIGHT_2=684,20
PRIMER_LEFT_2_TM=58.951
PRIMER_RIGHT_2_TM=61.375
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=4.6627
PRIMER_LEFT_3_PENALTY=1.287831
PRIMER_RIGHT_3_PENALTY=3.374827
PRIMER_LEFT_3_SEQUENCE=CACAATTGTTGACGCATAATCG
PRIMER_RIGHT_3_SEQUENCE=GCTCTGTGCTCCGTTTGGTA
PRIMER_LEFT_3=391,22
PRIMER_RIGHT_3=684,20
PRIMER_LEFT_3_TM=61.288
PRIMER_RIGHT_3_TM=61.375
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=4.8289
PRIMER_LEFT_4_PENALTY=1.454032
PRIMER_RIGHT_4_PENALTY=3.374827
PRIMER_LEFT_4_SEQUENCE=CCGAATCGTTGAATTGATGAG
PRIMER_RIGHT_4_SEQUENCE=GCTCTGTGCTCCGTTTGGTA
PRIMER_LEFT_4=448,21
PRIMER_RIGHT_4=684,20
PRIMER_LEFT_4_TM=60.454
PRIMER_RIGHT_4_TM=61.375
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_04992 Ps_contig_mira-and-tgicl-ass_franssen_35898
SEQUENCE=TTTTACAAAAACAGATACTTGTGAGTATACACCAATCGATGGTAGGTAACTCACAATCTTCTATGCGTGTTCAATTCTCAGATAACAGCTCTCTGGTGTAAAACTGAGTCTTTTTTTCTTTTCTGTAAGTTGGTTAGTTCTTGAATTTTCTTCCTTTGTGGAACAGGAAGAAAATTTGTTCTGACTTTTCATTCTCATTTTGTTTTTAAATTTAATTTAATTTGTTTTTAAAGTGTAGATTCTCTTTTCTTCTTTTTTTATTGGAAAGGAATGGGATCAGTGTGCACCAATGTATAAATTTTGATGAAAATNNTTTTTGTTTATTTTTTTTGTTGTTAGG
TARGET=209,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7395
PRIMER_LEFT_PENALTY=0.485895
PRIMER_RIGHT_PENALTY=0.253645
PRIMER_LEFT_SEQUENCE=CCTTTGTGGAACAGGAAGAAAA
PRIMER_RIGHT_SEQUENCE=ATACATTGGTGCACACTGATCC
PRIMER_LEFT=152,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=60.486
PRIMER_RIGHT_TM=59.746
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=143
PRIMER_PAIR_PENALTY_1=1.1704
PRIMER_LEFT_1_PENALTY=1.027078
PRIMER_RIGHT_1_PENALTY=0.143280
PRIMER_LEFT_1_SEQUENCE=CCAATCGATGGTAGGTAACTCA
PRIMER_RIGHT_1_SEQUENCE=CTGATCCCATTCCTTTCCAATA
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=279,22
PRIMER_LEFT_1_TM=58.973
PRIMER_RIGHT_1_TM=60.143
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=1.2389
PRIMER_LEFT_2_PENALTY=0.985281
PRIMER_RIGHT_2_PENALTY=0.253645
PRIMER_LEFT_2_SEQUENCE=TCCTTTGTGGAACAGGAAGAAA
PRIMER_RIGHT_2_SEQUENCE=ATACATTGGTGCACACTGATCC
PRIMER_LEFT_2=151,22
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=60.985
PRIMER_RIGHT_2_TM=59.746
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=1.2389
PRIMER_LEFT_3_PENALTY=0.985281
PRIMER_RIGHT_3_PENALTY=0.253645
PRIMER_LEFT_3_SEQUENCE=TTCCTTTGTGGAACAGGAAGAA
PRIMER_RIGHT_3_SEQUENCE=ATACATTGGTGCACACTGATCC
PRIMER_LEFT_3=150,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=60.985
PRIMER_RIGHT_3_TM=59.746
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=1.2807
PRIMER_LEFT_4_PENALTY=1.027078
PRIMER_RIGHT_4_PENALTY=0.253645
PRIMER_LEFT_4_SEQUENCE=CCAATCGATGGTAGGTAACTCA
PRIMER_RIGHT_4_SEQUENCE=ATACATTGGTGCACACTGATCC
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=58.973
PRIMER_RIGHT_4_TM=59.746
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_04993 Ps_contig_mira-and-tgicl-ass_franssen_35903
SEQUENCE=GAGATGTTACTCACGGGAAGGGGAGAACAAATCACGTGGTCTTAACACTGATAGTAAGATCATATAGAACCTCCAAATGTAAATTCTGGCAGAGCAATATAACATGCCTTAGGAAAACAAGATGGTAGTGCAAAGTTCCCTAATAAATTAGATGCGCAAGAACTGAGCATTGTGGACATCATGATGGTAGTGCAAAGTTCCCTAATTAATCCAGCTCTTATTAATATATTACATTGCAACTCTTAAAGCTTCTTTTGGCTCTCTTTCCACGCAAGCAATAAACTGAAAGATCGTGCGTTTTTGTATAATAATCCTGATTGTATGTGTAACTAACATTTTGCTTCCTGAAGTTCCAAATGACTTGCTGCAGTCAAGTTCTTAAAGTCCAAGATGTACTTACAGGAAAAGTTTAGAAAAATAATCAACCCAGAACGTCATATGGTTTCTGATTGTGGAAAGAGCTTTTCCAACTTGTTATTCGAAAGAATCCTATTGGGATCCAGTTCCTTTCTCGCCTTATTGTATACATCCACAGGAAACCGCTTTCTCAGCCTTGCCTGGAGAGCTACAAGCTCCTCCTTGTTCTTTGGAACCTCAATCTTTGCCCAGTGTTCATAAGCAGAGTAATCATCCCATAATTTTGCCTGAGTCAAATGCCTGTAATGAAAGAATTCTTCCGTTATATCTTTTCTCTGGCGAGCATCCATGGTAGGTAGGTACATGATTATGCCAACCCAAGAAAATATATCATCCTCTGACGGGCTTGAAGCAGGACTCAAGGGACTCCAGGTGCTAGCTGTCCATCGCTGCTCAATTGGAGCAGGAGCAGGTATCTCTTCTTTCTCTATAAGTTTCTTCAGCTCTTCAATGTATTCAAGATCTTTCATGCTTGGCTTTGCCAGTTTTCCAGCAGGGAAACATGTCTCTGATACCCACTGTTGGCCTCCACAATCAAAACCTAATATTTCATCACTCCATCCAACCCTATATCCTTCGGACTTCTTCCAGAACTCGGCCTCAACTTGATTGACCTTAGAACAAATGTGATGTTTATTAAAAGTAGGATCTATGGCAATCCCGCG
TARGET=708,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1364
PRIMER_LEFT_PENALTY=0.108385
PRIMER_RIGHT_PENALTY=0.028056
PRIMER_LEFT_SEQUENCE=CCGTTATATCTTTTCTCTGGCG
PRIMER_RIGHT_SEQUENCE=CCAACAGTGGGTATCAGAGACA
PRIMER_LEFT=676,22
PRIMER_RIGHT=941,22
PRIMER_LEFT_TM=60.108
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=11.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.3772
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.373576
PRIMER_LEFT_1_SEQUENCE=CAATCTTTGCCCAGTGTTCATA
PRIMER_RIGHT_1_SEQUENCE=GAAGAGATACCTGCTCCTGCTC
PRIMER_LEFT_1=595,22
PRIMER_RIGHT_1=838,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=59.626
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.3962
PRIMER_LEFT_2_PENALTY=0.368190
PRIMER_RIGHT_2_PENALTY=0.028056
PRIMER_LEFT_2_SEQUENCE=CCTGAGTCAAATGCCTGTAATG
PRIMER_RIGHT_2_SEQUENCE=CCAACAGTGGGTATCAGAGACA
PRIMER_LEFT_2=643,22
PRIMER_RIGHT_2=941,22
PRIMER_LEFT_2_TM=59.632
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=0.4516
PRIMER_LEFT_3_PENALTY=0.078029
PRIMER_RIGHT_3_PENALTY=0.373576
PRIMER_LEFT_3_SEQUENCE=ACAAGCTCCTCCTTGTTCTTTG
PRIMER_RIGHT_3_SEQUENCE=GAAGAGATACCTGCTCCTGCTC
PRIMER_LEFT_3=567,22
PRIMER_RIGHT_3=838,22
PRIMER_LEFT_3_TM=59.922
PRIMER_RIGHT_3_TM=59.626
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=272
PRIMER_PAIR_PENALTY_4=0.4820
PRIMER_LEFT_4_PENALTY=0.108385
PRIMER_RIGHT_4_PENALTY=0.373576
PRIMER_LEFT_4_SEQUENCE=CCGTTATATCTTTTCTCTGGCG
PRIMER_RIGHT_4_SEQUENCE=GAAGAGATACCTGCTCCTGCTC
PRIMER_LEFT_4=676,22
PRIMER_RIGHT_4=838,22
PRIMER_LEFT_4_TM=60.108
PRIMER_RIGHT_4_TM=59.626
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_04994 Ps_contig_mira-and-tgicl-ass_franssen_35927
SEQUENCE=GGAAAGTCAACACAAAACCCTTACTTGGNTTCCATCATCANGTTATTTCTTCAATATTATACATTCTTATCTCTATATGCTTTCATCAATGCCAAGCTGAAGCTGATATCAAATTTCTGTTCTTCCACCCCAACAACATGGCTCCATCTTTCTCCACTAATGTATAATGCTCTGAATAGCATCTCAGAAGACTTCTTATAACAGAATTCACATAAGAACTCAAAGGACATTGAGTAAATCCGGCCATTGTCAGCCTAGACTTCCACTTGCCGAACAGTTCGTGCCGCTCGATCCTTTCCTTTCCTTCACAAGCAATGACATTGACGATATCGCGAGACCAAACAATGCTGCTCCACGTTAATCCTCTCCTTGCTGTTTC
TARGET=292,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3122
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.216588
PRIMER_LEFT_SEQUENCE=ATGGCTCCATCTTTCTCCACTA
PRIMER_RIGHT_SEQUENCE=AACAGCAAGGAGAGGATTAACG
PRIMER_LEFT=137,22
PRIMER_RIGHT=376,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.783
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=7.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.4554
PRIMER_LEFT_1_PENALTY=0.332137
PRIMER_RIGHT_1_PENALTY=0.123278
PRIMER_LEFT_1_SEQUENCE=TCAAATTTCTGTTCTTCCACCC
PRIMER_RIGHT_1_SEQUENCE=CAAGGAGAGGATTAACGTGGAG
PRIMER_LEFT_1=108,22
PRIMER_RIGHT_1=371,22
PRIMER_LEFT_1_TM=60.332
PRIMER_RIGHT_1_TM=60.123
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.5487
PRIMER_LEFT_2_PENALTY=0.332137
PRIMER_RIGHT_2_PENALTY=0.216588
PRIMER_LEFT_2_SEQUENCE=TCAAATTTCTGTTCTTCCACCC
PRIMER_RIGHT_2_SEQUENCE=AACAGCAAGGAGAGGATTAACG
PRIMER_LEFT_2=108,22
PRIMER_RIGHT_2=376,22
PRIMER_LEFT_2_TM=60.332
PRIMER_RIGHT_2_TM=59.783
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=0.5674
PRIMER_LEFT_3_PENALTY=0.444124
PRIMER_RIGHT_3_PENALTY=0.123278
PRIMER_LEFT_3_SEQUENCE=GTCAGCCTAGACTTCCACTTGC
PRIMER_RIGHT_3_SEQUENCE=CAAGGAGAGGATTAACGTGGAG
PRIMER_LEFT_3=248,22
PRIMER_RIGHT_3=371,22
PRIMER_LEFT_3_TM=60.444
PRIMER_RIGHT_3_TM=60.123
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=0.5850
PRIMER_LEFT_4_PENALTY=0.461740
PRIMER_RIGHT_4_PENALTY=0.123278
PRIMER_LEFT_4_SEQUENCE=TGTCAGCCTAGACTTCCACTTG
PRIMER_RIGHT_4_SEQUENCE=CAAGGAGAGGATTAACGTGGAG
PRIMER_LEFT_4=247,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=59.538
PRIMER_RIGHT_4_TM=60.123
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_04995 Ps_contig_mira-and-tgicl-ass_franssen_35931
SEQUENCE=ACTTCAATTTCAACATTTYCTNTAAGAAGCAATTAGTACTAGTATTTAATCAAGTTGAATTTCAATCTCAATTTGACAAACAATAGACAATGTAACAAAAAAACATAGATTAGGAATCATCATACAACAGATCAAAACCTATTAAGTACAAGAAACACTAAATTTACAAACACCAAGATAATTCTCTTCTCACAAGAAGATCTTACTTATTACCATCTCCAATGGTTTTATTATCTACAACAATCTTAACTTTTCTATAATCACCCTCCAAAGCTTGCATAACACATCCTTCTGGAACCACTGCGCCACCGCGTTTATTGCCAAAGATTGTCCACAAATAAAGCACAGTGAATGCTGTCAAAGCTCCACAACCAGTACCAAATATCATTGCAGCAAGAACTCTTAAAAAACAATCACTCTTTCCCTTTTCCTTTGATTCAACAACCATTGGATTCTCACTGTTTTTCACAAAAACCTTAGGATCAAGTGGCTCTGAATGCATAGAATGTGGTGGAAAATGTCTCATGACAAAGCTCCATGAGTACAAAAAAGCATTAGTCTCTCTCACAGGACTAAAACCTACAAACATTTCTTCCTCCTTCAACACGTTAGATACTTCAATTTTGTCCCATAACAATGGATCAAATGGCTTTAAATTCCCATTTTTACTCAACCTCACTTCTAATCTCATTGAACTTGCTTCATAATCAATCCAAGTATGCATCTTTTCACCACTTGATAGTGCCAAATTGGTAAAGTAGATTTTACTTGAATCACCATTTCTAATCAAAATCTCATTTTTACCATCCCTTGAAGCACTAAACTCAACACCAATAACTTCAAAATCTTTATTTTTCAATCCAAGAGAAAACCCAGATGAAGTTTGATTAAAAACATCACCTTTAACACCATTTGGAATTATAACAAAAGCTAAACCACCCCCACTTTCCAATGAGATTGAAAATGTAAAGTAAGTTGAAAATGAAACTAATTGCTTAGGTTTACCTTGAAAAAGCTTGATGGGTTTGTTGTAAATGACTCTCCCAGACTCAGCACCAAAACTACCTGAACCAGAGAGTTGAATCTTGGACTCACCATTGACAACCTTTGCATTACCAAAAAAAGCAACATTAGACTTAAATTTCAGATCTTTTTCAAAATCAACAAAGGAAAACAATGGAATTGGGTCAAAAGATAGGATTTTTATGAATAAAGTGATGAAAATGAAGGATTTGAAGTAATGGGTAGTGGTGAATGATGCCATAGCTAAGAGTTGGATAGTTTATGATTCTAGGGTTGGATGGTTGATTTTGATTCCATTTGAAGCATAAAGGTTGAAACTTTGTGATGAGAGGAAGAAAGTGAAGGAGTATTGGGAAAGTTGGATTGGGGGGTGTGAAAATTAAATGGATTTTGTTGTATCCCGAACATGGTGGTGGTGGTTCACTTTTATTTATTTTCCTC
TARGET=1431,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_04996 Ps_contig_mira-and-tgicl-ass_franssen_35950
SEQUENCE=AATCTGATTATGATTATTATTATTTTTATCAGATGAAAATGAAGTTCCTTTTTTACAACTTAATGGTGATTCTTCTTCAAAAGGATTCTCATTTTTGCTTGATAGTGCTGAATCACTACTTTCCCCTCCTGATTTGTTTCCTCCTATTGAGGTTTGATGGAATCTCATTGGCCAAGAAGGGAACATTTCCAGTGTAGCAGAAGGTCTGGCTGTGAAAAAAGATGCTTTTGTTTCATCATTTCTACTCTTAACTCCTTGCAGAACTATTGCTTCTTCTAGCTCCCCAAAATCAA
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_04997 Ps_contig_mira-and-tgicl-ass_franssen_35951
SEQUENCE=ATTAAAACAANNTGCAAGAACTAGTATTGCATGGTATGATTGACTCATCCAGAAGTTATTTAAAATTACAGAAGCAAAAGTACATTGCTCATTTAACTCGAGAAAAATATATCACAAAGGCAAACCAAACTGATAAATAACCTAGAATATTCACAGATGATGGAGACCTGAAAAGGGTATATGACTACAAACTCAAACATCTTACAAGATCAGTCTAGTTGCAGGTCTCTTATAAAAGTATTGCCACAAACTTTTAAAAACCAGCAGATGCTTCGGTTTCTCAGTTCAAGCAATATGGGTAAATGATTTGCAGCTCTTTAAATAATAATAACTACTACTCATATGGGGCCGCAGGTGACCAGACGCTTGAAAGAGCAAGATTCAGGTGGCCGGGTTGTTGCTTCCAAACAAAATGTGTTTTCTGATTCTTCCTATATTAGGAACTTCATGTTCCCCATTATCAAATGATTCTACATTTCCTTAGATGGGAGCAAAAAGCATCAAAAGGAATTCATCTCAAGGCAATAAGTCCTCCTCTTAATAAGCATTGTAGCAGCAGACCTATAAGGCTCTTCGAAAGCAGTAGAAACCCAAGACAGATCAGATCCTTCCTTCTTAGCAGTTGTAGGTGACATCTCACTAGGCCTGATAGCAACAAGAGTAGCTCCTTTCAAAACAACCCCATCAGGCAACTCCAAATGAGGTGCATACCAAAGCCTCATATTCAAAGCAGGCACAAGAGTCCTCTTTGAAGCAGAAGAAGCCGAAAGCGGCTTCACCCTCAACTCTTCAAGTTGATCTCTGTTCATATACAACACACCTTGCCCATCTGCATCAGTCAACACCAAATTATCCAAAGTCTTATGCTCCGAAATAATCGGTTGCAACAAGTAATGCCTTGCAGATGCAGCAATCAACGAACTAATCGTCCAAACTACCCTCAATTTCAAACCACCGTTTGTATAAAACGAATCAGGTATACTTCCATTATCATCACCACCACCACCATTGTTATTTCCACCACCACCACAAGATGCATCAACAACACCACCATCTTGAGATTTAGGGTAGAAAAACAAGAAGGAACGTA
TARGET=995,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3723
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.276812
PRIMER_LEFT_SEQUENCE=ATCCAAAGTCTTATGCTCCGAA
PRIMER_RIGHT_SEQUENCE=TCAAGATGGTGGTGTTGTTGAT
PRIMER_LEFT=851,22
PRIMER_RIGHT=1058,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=60.277
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.4054
PRIMER_LEFT_1_PENALTY=0.128591
PRIMER_RIGHT_1_PENALTY=0.276812
PRIMER_LEFT_1_SEQUENCE=CGTCCAAACTACCCTCAATTTC
PRIMER_RIGHT_1_SEQUENCE=TCAAGATGGTGGTGTTGTTGAT
PRIMER_LEFT_1=926,22
PRIMER_RIGHT_1=1058,22
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=60.277
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=0.4054
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=0.276812
PRIMER_LEFT_2_SEQUENCE=TCGTCCAAACTACCCTCAATTT
PRIMER_RIGHT_2_SEQUENCE=TCAAGATGGTGGTGTTGTTGAT
PRIMER_LEFT_2=925,22
PRIMER_RIGHT_2=1058,22
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=60.277
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=0.6467
PRIMER_LEFT_3_PENALTY=0.369874
PRIMER_RIGHT_3_PENALTY=0.276812
PRIMER_LEFT_3_SEQUENCE=CCAAAGTCTTATGCTCCGAAAT
PRIMER_RIGHT_3_SEQUENCE=TCAAGATGGTGGTGTTGTTGAT
PRIMER_LEFT_3=853,22
PRIMER_RIGHT_3=1058,22
PRIMER_LEFT_3_TM=59.630
PRIMER_RIGHT_3_TM=60.277
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.6553
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.651681
PRIMER_LEFT_4_SEQUENCE=CAATCAACGAACTAATCGTCCA
PRIMER_RIGHT_4_SEQUENCE=CCCTAAATCTCAAGATGGTGGT
PRIMER_LEFT_4=910,22
PRIMER_RIGHT_4=1067,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=59.348
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_04998 Ps_contig_mira-and-tgicl-ass_franssen_35951
SEQUENCE=ATTAAAACAANNTGCAAGAACTAGTATTGCATGGTATGATTGACTCATCCAGAAGTTATTTAAAATTACAGAAGCAAAAGTACATTGCTCATTTAACTCGAGAAAAATATATCACAAAGGCAAACCAAACTGATAAATAACCTAGAATATTCACAGATGATGGAGACCTGAAAAGGGTATATGACTACAAACTCAAACATCTTACAAGATCAGTCTAGTTGCAGGTCTCTTATAAAAGTATTGCCACAAACTTTTAAAAACCAGCAGATGCTTCGGTTTCTCAGTTCAAGCAATATGGGTAAATGATTTGCAGCTCTTTAAATAATAATAACTACTACTCATATGGGGCCGCAGGTGACCAGACGCTTGAAAGAGCAAGATTCAGGTGGCCGGGTTGTTGCTTCCAAACAAAATGTGTTTTCTGATTCTTCCTATATTAGGAACTTCATGTTCCCCATTATCAAATGATTCTACATTTCCTTAGATGGGAGCAAAAAGCATCAAAAGGAATTCATCTCAAGGCAATAAGTCCTCCTCTTAATAAGCATTGTAGCAGCAGACCTATAAGGCTCTTCGAAAGCAGTAGAAACCCAAGACAGATCAGATCCTTCCTTCTTAGCAGTTGTAGGTGACATCTCACTAGGCCTGATAGCAACAAGAGTAGCTCCTTTCAAAACAACCCCATCAGGCAACTCCAAATGAGGTGCATACCAAAGCCTCATATTCAAAGCAGGCACAAGAGTCCTCTTTGAAGCAGAAGAAGCCGAAAGCGGCTTCACCCTCAACTCTTCAAGTTGATCTCTGTTCATATACAACACACCTTGCCCATCTGCATCAGTCAACACCAAATTATCCAAAGTCTTATGCTCCGAAATAATCGGTTGCAACAAGTAATGCCTTGCAGATGCAGCAATCAACGAACTAATCGTCCAAACTACCCTCAATTTCAAACCACCGTTTGTATAAAACGAATCAGGTATACTTCCATTATCATCACCACCACCACCATTGTTATTTCCACCACCACCACAAGATGCATCAACAACACCACCATCTTGAGATTTAGGGTAGAAAAACAAGAAGGAACGTA
TARGET=1018,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3723
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.276812
PRIMER_LEFT_SEQUENCE=ATCCAAAGTCTTATGCTCCGAA
PRIMER_RIGHT_SEQUENCE=TCAAGATGGTGGTGTTGTTGAT
PRIMER_LEFT=851,22
PRIMER_RIGHT=1058,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=60.277
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.4054
PRIMER_LEFT_1_PENALTY=0.128591
PRIMER_RIGHT_1_PENALTY=0.276812
PRIMER_LEFT_1_SEQUENCE=CGTCCAAACTACCCTCAATTTC
PRIMER_RIGHT_1_SEQUENCE=TCAAGATGGTGGTGTTGTTGAT
PRIMER_LEFT_1=926,22
PRIMER_RIGHT_1=1058,22
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=60.277
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=0.4054
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=0.276812
PRIMER_LEFT_2_SEQUENCE=TCGTCCAAACTACCCTCAATTT
PRIMER_RIGHT_2_SEQUENCE=TCAAGATGGTGGTGTTGTTGAT
PRIMER_LEFT_2=925,22
PRIMER_RIGHT_2=1058,22
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=60.277
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=0.6467
PRIMER_LEFT_3_PENALTY=0.369874
PRIMER_RIGHT_3_PENALTY=0.276812
PRIMER_LEFT_3_SEQUENCE=CCAAAGTCTTATGCTCCGAAAT
PRIMER_RIGHT_3_SEQUENCE=TCAAGATGGTGGTGTTGTTGAT
PRIMER_LEFT_3=853,22
PRIMER_RIGHT_3=1058,22
PRIMER_LEFT_3_TM=59.630
PRIMER_RIGHT_3_TM=60.277
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.6553
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.651681
PRIMER_LEFT_4_SEQUENCE=CAATCAACGAACTAATCGTCCA
PRIMER_RIGHT_4_SEQUENCE=CCCTAAATCTCAAGATGGTGGT
PRIMER_LEFT_4=910,22
PRIMER_RIGHT_4=1067,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=59.348
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_04999 Ps_contig_mira-and-tgicl-ass_franssen_35952
SEQUENCE=ATTGAGNCGNGRGRGAGAGAGAGAAAAGATAGATGGGGCTAGCTACAGTTAGAGCATTTTGCGTCTGTGCAAACCAACCTCTTCTTCATCCCCCGGCTCACTGCCATTTCCTTCCAACCTCATCTCTTTTCTCCTCCTTTTCTTTTCAGAAAAACTAACAAATTCAATCTTCAAAAATCTATTCAACATGCTGCAAACTCAAAACTATCATGTAGTTTACTCAACGTGGAAAGTGACATAAATGATGAAGCATGTGAATTAGTCAGTGGAGAGGAGCTTTCGCTTGAGGACGACGATGATAACATTCACGCTTATCTCTTCAAGGCTGTCAAAAATAATAACGGAATTGGCCTATTGCTTCTTTCAGATATTTATGGCTTCGAGGATTCCTTCACAAGAGATTTCGCATATCGGGTAGCTTGCAATGGTTTCAACATTCTAGTTCCTGACTTATTTCGCGGAAATCCGTGGACAAAGGATCAGCCAAACACTCTGTTGGAGCAGTGGATTGCTAGACATAACCCTGAGAGGATTGCAAAAGATATTACTGCATGGACAGAATGGATGACAGATGAATTCTTGACTTCGGGAGATTCCAGAAAACTTAGCATAATTGGTTTTTGTTTTGGAGGTGGCCTACTGTTAGAGGTGTTAGCGCGAGACGAAGGCGCGTGTTTTGGCACTGGAATCTCCTTCTATGGTACAAGGATAGATCCTGTTGTTGCTTCTGATGTAAAAGTTCCTGTCTTGTTTATTTTAGGGGACAGTGATCCATTTTGTGCTGTAAGTGAAATAGAAAATATTCAGAAGAAAATGGATAGAGGTTCAAAAGTAGTGATATTCCCAGGGAGAGGTCATGGATTTGCCCACCGACCGGGATCTCCCGAAGAAGATAAGGATGCTGAAAAAGCTTATGTAATTATGAGAGACTGGATATATGAACACTTGGTCGTGTAAATAACAGTAGCATATGTATATGATTTTTGAGTTTCTAGACTTTACTGTCAACTCACATTTTGGTTGTAAGAACTTTAATCTTTAAAGGTTAGTAAGAATCCTTTGACATTGTTTGTCTAAAAT
TARGET=15,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05000 Ps_contig_mira-and-tgicl-ass_franssen_35955
SEQUENCE=TCGGGGNGAAGTTCATCATAGGGTTTCCTTATTATTTCCATTAGTGAACATTTGAATCTCACTATGGAGGGACATGAAATTTTGGGGTTCGGTTTCTTTGTAATTGGTTTATGGCACTTTTTCAACCACATTAAGCTCCATTTTCTTTCTTCTTCTTCTCTCAAATCCTACTCATCAACCCTATGGTTTCCAACCAAACTAAGTCGATACCTCGAGCTTCATTTCACCATGGCAAGTTGCATGATCTTCATATCTATGGAACTTTTCATCTCACCTCTTCATCACCAACCATTTGACCCTAAAGATGGAACCATTCCCTCTAACCATCTTCACAACTTCGAACACTCTTCCATGGCTTTGTCTTTCTTACTCTATGCTACATTTTCTTATAATTCTTGATAAGAAAAATAAACCATTGCTAAAAGAAACTCAAAATAGACTCACTCATTTGT
TARGET=147,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2770
PRIMER_LEFT_PENALTY=0.247794
PRIMER_RIGHT_PENALTY=0.029253
PRIMER_LEFT_SEQUENCE=GGTTCGGTTTCTTTGTAATTGG
PRIMER_RIGHT_SEQUENCE=TCAAATGGTTGGTGATGAAGAG
PRIMER_LEFT=85,22
PRIMER_RIGHT=295,22
PRIMER_LEFT_TM=59.752
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=0.2770
PRIMER_LEFT_1_PENALTY=0.247794
PRIMER_RIGHT_1_PENALTY=0.029253
PRIMER_LEFT_1_SEQUENCE=GGGTTCGGTTTCTTTGTAATTG
PRIMER_RIGHT_1_SEQUENCE=TCAAATGGTTGGTGATGAAGAG
PRIMER_LEFT_1=84,22
PRIMER_RIGHT_1=295,22
PRIMER_LEFT_1_TM=59.752
PRIMER_RIGHT_1_TM=59.971
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.3589
PRIMER_LEFT_2_PENALTY=0.247794
PRIMER_RIGHT_2_PENALTY=0.111077
PRIMER_LEFT_2_SEQUENCE=GGTTCGGTTTCTTTGTAATTGG
PRIMER_RIGHT_2_SEQUENCE=TTGGTGATGAAGAGGTGAGATG
PRIMER_LEFT_2=85,22
PRIMER_RIGHT_2=287,22
PRIMER_LEFT_2_TM=59.752
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.3589
PRIMER_LEFT_3_PENALTY=0.247794
PRIMER_RIGHT_3_PENALTY=0.111077
PRIMER_LEFT_3_SEQUENCE=GGGTTCGGTTTCTTTGTAATTG
PRIMER_RIGHT_3_SEQUENCE=TTGGTGATGAAGAGGTGAGATG
PRIMER_LEFT_3=84,22
PRIMER_RIGHT_3=287,22
PRIMER_LEFT_3_TM=59.752
PRIMER_RIGHT_3_TM=60.111
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.4060
PRIMER_LEFT_4_PENALTY=0.242357
PRIMER_RIGHT_4_PENALTY=0.163658
PRIMER_LEFT_4_SEQUENCE=GGTTTATGGCACTTTTTCAACC
PRIMER_RIGHT_4_SEQUENCE=GTCAAATGGTTGGTGATGAAGA
PRIMER_LEFT_4=105,22
PRIMER_RIGHT_4=296,22
PRIMER_LEFT_4_TM=59.758
PRIMER_RIGHT_4_TM=59.836
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_05001 Ps_contig_mira-and-tgicl-ass_franssen_35964
SEQUENCE=AAACTCTGACCCTCCACCTTATACAAAAACTTCCTCCTTATTTGTTATTTTGTTTCGGAAGCTTTGACTGTTCAACTAACTCATCTGCAGTAATATCCATGAAAGCTTCCACAAACAAAGCACCTTTTCCATTGCAATCCACCTCAAGCTTCTTCTCATCATCAATATCATCGTCCAAACTAGAGGAACATCTTCTCAACCTTCCTGCTAAGGGGTAATAATCCACTAAAACTCTAGACAGAGAAGATTTCAAGTTATTCAAGTTCACTGATTTCTTGAAAATGTATAAATACTTGATTGAAAATCTAAGAAACTTTTGGTCATCGAGATTGGAAAGATAGAGAGAGTGTTTTGGCGTTGGAGCACTTGGTGGAATTTTAGTGACGGGTTCACATGGATAGGTACAATTAGGGATTCCTTTGATTTAGACATGATAATTTTTATGATGATGATGATTATGATCTATAACTATTATAGTAATAAAGAGTCATAAACAGAGAGAGAAAGAGATAATGAGAAGATGATTGAATCAAAGAGTGGATTGAGAAAACTTGTATAAATAATATTTTGAGATAGATGTGAATGTGACAAGACATTAGTGTAGGACCACTCCAAGTGGGGTATCA
TARGET=443,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4609
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.460175
PRIMER_LEFT_SEQUENCE=GGAGCACTTGGTGGAATTTTAG
PRIMER_RIGHT_SEQUENCE=CCACTTGGAGTGGTCCTACACT
PRIMER_LEFT=359,22
PRIMER_RIGHT=618,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.460
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.7091
PRIMER_LEFT_1_PENALTY=0.248900
PRIMER_RIGHT_1_PENALTY=0.460175
PRIMER_LEFT_1_SEQUENCE=TAGTGACGGGTTCACATGGATA
PRIMER_RIGHT_1_SEQUENCE=CCACTTGGAGTGGTCCTACACT
PRIMER_LEFT_1=378,22
PRIMER_RIGHT_1=618,22
PRIMER_LEFT_1_TM=60.249
PRIMER_RIGHT_1_TM=60.460
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.7135
PRIMER_LEFT_2_PENALTY=0.253332
PRIMER_RIGHT_2_PENALTY=0.460175
PRIMER_LEFT_2_SEQUENCE=GTGGAATTTTAGTGACGGGTTC
PRIMER_RIGHT_2_SEQUENCE=CCACTTGGAGTGGTCCTACACT
PRIMER_LEFT_2=369,22
PRIMER_RIGHT_2=618,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.460
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.9031
PRIMER_LEFT_3_PENALTY=0.442900
PRIMER_RIGHT_3_PENALTY=0.460175
PRIMER_LEFT_3_SEQUENCE=TAGAGAGAGTGTTTTGGCGTTG
PRIMER_RIGHT_3_SEQUENCE=CCACTTGGAGTGGTCCTACACT
PRIMER_LEFT_3=338,22
PRIMER_RIGHT_3=618,22
PRIMER_LEFT_3_TM=59.557
PRIMER_RIGHT_3_TM=60.460
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=1.3258
PRIMER_LEFT_4_PENALTY=0.865663
PRIMER_RIGHT_4_PENALTY=0.460175
PRIMER_LEFT_4_SEQUENCE=GCACTTGGTGGAATTTTAGTGA
PRIMER_RIGHT_4_SEQUENCE=CCACTTGGAGTGGTCCTACACT
PRIMER_LEFT_4=362,22
PRIMER_RIGHT_4=618,22
PRIMER_LEFT_4_TM=59.134
PRIMER_RIGHT_4_TM=60.460
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_05002 Ps_contig_mira-and-tgicl-ass_franssen_35968
SEQUENCE=GACCTCTCTTTCATTCCAAATCAAAATCTTTTCGCAACACTACAAATACATTCCATGACTCTTTCTTTCTTTCTGCAATTCCAATGTTATCATCTTAACACGACAAAAACAYNNGNTATTAACAACAACACCAACCTTAACCTTCTTCGCTTCTTACTTTTGTCTTGTTTTGTTTTCTAGATAACTATTACTACTGCATACTAATACATATACTTAATTGTGTAATTAATCATAATTAGTAACGATAATTAGTTACGACTTTAATTAAACGTCGCGAATGGCGGTTGTTTTTGTGATAACGCTCGCGATGCTAACGGCGGCGACGGTTATTTCTGGATCGCCGGTGACTCTCACGTTGGAAAGAGCGTTTCCGACGAATCATGGAGTGGAGTTGAATCAGCTCAGAGCGAGAGATTTGTTGAGACATCGTAGAATTCTGCAGTCTTCAAACGGTGTTGTTGATTTCGATGTTCATGGTACCTTCGATCCCTTCCAAGTCGGGCTATATTTTACAAAGGTGCAGTTAGGTACTCCTCCAGTAGAATTTTATGTGCAAATTGACACTGGAAGTGATGTTCTTTGGGTCAGTTGCAGCTCCTGCACTGGTTGTCCACAGACAAGTGGGCTACAGATTGAGCTCAATTTTTTCGACTCTGGGCGTTCGTCGACATCATCGTTAATCTCTTGTTCTGACCGAAGGTGCAACAGTGGAATACA
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05003 Ps_contig_mira-and-tgicl-ass_franssen_35973
SEQUENCE=AATGTTCACATAATCTTCCTACTACAAAACCCCCCAACACAACAACATATTCNAACAACAACAACAAACCTTGTCTCTCTTCTCTCCCTCTCTGTAACCATGTCATCATCAGTAACACCGATTCTGARAGATGAACTGGACATCGTCATTCCAACAATAAGGAACCTCGATTTCTTGGAACAGTGGAGACCATTCTTCCAACCATATCATCTCATCATCATACAAGATGGTGATCCTTCTAAAGTCATCAAAGTCCCTCAAGGCTTTGATTATGAGCTTTATAATCGTAACGATATTAACCGTATTTGGGTCCTAAGGCTTCTTGTATTTCATTCAAAGACTCGGCTTGTCGCTGCTTTGGTTTCGCCATCTCCAAAAAAAAATACATCTTTACCATTGACGATGACTGCTTTG
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05004 Ps_contig_mira-and-tgicl-ass_franssen_35976
SEQUENCE=GTTTTATTGGTTCTTTGAATCTGTTGATCAACCTCAACAAAAACCTCTTCTCCTTTGGCTTAATGGAGGCCCTGGTTGTTCTTCAATTGGTTATGGAGAAGCAGAGGAGCTTGGACCCTTTTCTCCTCAAAATAGCACCCAACCTAAGCTCAAGTTGAACCCTTATTCTTGGAATAAAGCTGTCAAATCTTTTATTTATTGAATCTCCTGTTGGAGTTGGATTTTCCTACACCAACACCACCACTGATATTAGTCAACTTGGTGACACCATTACTGCTAAGGATTCACACACTTTCCTCATCAATTGGTTTAAAAGATTTCCTCAATTCAAATCACATGATTTCTACATTGCTGGTGAAAGCTATGCAGGACATTATGTTCCACAACTTTCTGAACTCATTTTGGATAATAATAATAATCAGAACTCTTCCGATGAAGATCACATTAACTTCAAAGGGATCATGATTGGGAATGCATYATTGGATGATGAAACTGATACGAAAAGGGTATGATAGAATATCGCAGTGGG
TARGET=406,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05005 Ps_contig_mira-and-tgicl-ass_franssen_35992
SEQUENCE=AAAGACAAGTAGGTAATACAAACGACAGAAGNANGGGGGAAAACAATTGAGAGAGCATTTTATGTAGCAAAAGTTTCAGTTTCACAATTGTTGCTCATTCTTATGGTAGTTTTGTGGAACTGTTTTTATCACTCATACTCAAATGTTGGGTGGTGCACTCTCAACTCCAACAACTCAACGCCTCAAGTCAACAACAACAACAATTACAATGCTTTATTCATCTCATAGATTGTCCACACAATTTTGTCTCTTTAAGAATCTTATAGCAGGTAATAGAAGATTTTCCTCTAATGAAAGTTGTTTTCTCAGAGGTCACCAAATTAATATCAAGCATGTTTTTGGGCTTATCTCTCGTCAAGCACCAAGAAACAAAGCAGGATTTGTTGTTTCTCCAAGATGTGGTCTACCACTGACAGAAGAGAATGTGGAGAGGGTTTTGGATGAGGTAAGGCCTGGCTTGATGGCTGATGGAGGGAATGTGGCCTTACATGAGATAGATGGTCTTGTTGTTATCCTCAAACTACAAGGAGCATGTGGATCATGTCCTAGCTCAACCATGACATTAAAGATGGGGATCGAAACTCGCCTTCGCGATAAGATTCCGGAAATCATCGAAGTTGAGCAAATACTAGATACCGAGACTGGTCTTGAGTTAACCGAAGAGAATGTTGAAAGTATTCTTTCGGAAATTAGACCGTATCTTGTTGGCACTGGAGGAGGAACATTAGAGCTTGTTGAGATCAGTGATTATACCGTTAAAGTTCGGTTAAGTGGACCGGCTGCAGGAGTCATGACGGTTCGCGTTGCCTTGACGCAGAAGCTGCGAGACAAGATACCTTCTATTGCTGCTGTGCAGCTGATAGAATGATGTAACATATTAGTTTGTGTATGTTTTGATAAATCAATACAAAGATGTAAACTTGTCTTGAGAAAAGAAATTCAGTGTCATATAGTTATACATATTTGATTTGAGTTTTGCATAAGATTATGATTAGCTAGCCAATCTACGTTTCAGTTGTCTTAAAGATGAAGTAAGAGGCATTTCCCTTCAATCGTTAAGACATCTTTCACATCAATGATACTAAGTCTGTCATAGACTTTCTCAAGATCATTCTTA
TARGET=189,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0551
PRIMER_LEFT_PENALTY=0.046801
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=TGCACTCTCAACTCCAACAACT
PRIMER_RIGHT_SEQUENCE=CCACATCTTGGAGAAACAACAA
PRIMER_LEFT=153,22
PRIMER_RIGHT=401,22
PRIMER_LEFT_TM=59.953
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.0749
PRIMER_LEFT_1_PENALTY=0.046801
PRIMER_RIGHT_1_PENALTY=0.028056
PRIMER_LEFT_1_SEQUENCE=TGCACTCTCAACTCCAACAACT
PRIMER_RIGHT_1_SEQUENCE=TCAGTGGTAGACCACATCTTGG
PRIMER_LEFT_1=153,22
PRIMER_RIGHT_1=412,22
PRIMER_LEFT_1_TM=59.953
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=260
PRIMER_PAIR_PENALTY_2=0.1759
PRIMER_LEFT_2_PENALTY=0.046801
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=TGCACTCTCAACTCCAACAACT
PRIMER_RIGHT_2_SEQUENCE=ACGAGAGATAAGCCCAAAAACA
PRIMER_LEFT_2=153,22
PRIMER_RIGHT_2=354,22
PRIMER_LEFT_2_TM=59.953
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.1924
PRIMER_LEFT_3_PENALTY=0.184188
PRIMER_RIGHT_3_PENALTY=0.008257
PRIMER_LEFT_3_SEQUENCE=GTGCACTCTCAACTCCAACAAC
PRIMER_RIGHT_3_SEQUENCE=CCACATCTTGGAGAAACAACAA
PRIMER_LEFT_3=152,22
PRIMER_RIGHT_3=401,22
PRIMER_LEFT_3_TM=59.816
PRIMER_RIGHT_3_TM=60.008
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.2122
PRIMER_LEFT_4_PENALTY=0.184188
PRIMER_RIGHT_4_PENALTY=0.028056
PRIMER_LEFT_4_SEQUENCE=GTGCACTCTCAACTCCAACAAC
PRIMER_RIGHT_4_SEQUENCE=TCAGTGGTAGACCACATCTTGG
PRIMER_LEFT_4=152,22
PRIMER_RIGHT_4=412,22
PRIMER_LEFT_4_TM=59.816
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=261
=
PRIMER_SEQUENCE_ID=SSR_05006 Ps_contig_mira-and-tgicl-ass_franssen_35995
SEQUENCE=CTTGAATACGAGCTTTCAGCTCTTCGAGAAGCAATGGATTCGGGATTCACTTATTTGCTCGGCCATGGAGATGTGAGGGCAAAGAAGGACTCATTTTTCTTTAAGAAATTGATGATAAACTATTTCTATGCATTTCTGAGAAAGAATTGCAGAGGAGGTACTGCAAATATGAAAGTACCTCACACCAATATCATTCAAGTGGGGATGACATATATGGTTTGAACTATGAATGAATGAGCCATGCAATGTAATCTCAGTTTAAAACAACACATTGTTATTGTTATTGTTGTTGATGTTTTTCTTTCTGTTAACAATGATTGGAAATATTGTTGGAGTTATGTTGGCTTAAATATTTATTTTGGTCTTTAGAGCACTCAAATTGTGTTGAGTATCAGTTACTGAAGTGATGGAGCCAACATAGTTTATGTATGAAATATTTTCTTTCAATTTTAATAATCTTAAGAATTAGTTTCAA
TARGET=226,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2190
PRIMER_LEFT_PENALTY=0.086698
PRIMER_RIGHT_PENALTY=0.132262
PRIMER_LEFT_SEQUENCE=AGAATTGCAGAGGAGGTACTGC
PRIMER_RIGHT_SEQUENCE=CTATGTTGGCTCCATCACTTCA
PRIMER_LEFT=142,22
PRIMER_RIGHT=420,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=60.132
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.2190
PRIMER_LEFT_1_PENALTY=0.086698
PRIMER_RIGHT_1_PENALTY=0.132262
PRIMER_LEFT_1_SEQUENCE=AGAATTGCAGAGGAGGTACTGC
PRIMER_RIGHT_1_SEQUENCE=TATGTTGGCTCCATCACTTCAG
PRIMER_LEFT_1=142,22
PRIMER_RIGHT_1=419,22
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=60.132
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.2228
PRIMER_LEFT_2_PENALTY=0.090580
PRIMER_RIGHT_2_PENALTY=0.132262
PRIMER_LEFT_2_SEQUENCE=CCAATATCATTCAAGTGGGGAT
PRIMER_RIGHT_2_SEQUENCE=TATGTTGGCTCCATCACTTCAG
PRIMER_LEFT_2=184,22
PRIMER_RIGHT_2=419,22
PRIMER_LEFT_2_TM=59.909
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.2278
PRIMER_LEFT_3_PENALTY=0.095538
PRIMER_RIGHT_3_PENALTY=0.132262
PRIMER_LEFT_3_SEQUENCE=TGCAGAGGAGGTACTGCAAATA
PRIMER_RIGHT_3_SEQUENCE=CTATGTTGGCTCCATCACTTCA
PRIMER_LEFT_3=147,22
PRIMER_RIGHT_3=420,22
PRIMER_LEFT_3_TM=59.904
PRIMER_RIGHT_3_TM=60.132
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.2278
PRIMER_LEFT_4_PENALTY=0.095538
PRIMER_RIGHT_4_PENALTY=0.132262
PRIMER_LEFT_4_SEQUENCE=TGCAGAGGAGGTACTGCAAATA
PRIMER_RIGHT_4_SEQUENCE=TATGTTGGCTCCATCACTTCAG
PRIMER_LEFT_4=147,22
PRIMER_RIGHT_4=419,22
PRIMER_LEFT_4_TM=59.904
PRIMER_RIGHT_4_TM=60.132
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_05007 Ps_contig_mira-and-tgicl-ass_franssen_36001
SEQUENCE=CGGGTCCGTATTCACCGTCCGGAGGACTCCATTGGATCCAAAATACCTTCCAGAATGCAGAAGTATCACTCCATTGCTGATGTCGCTCTTCAGCTTAAAATTGTCGCGGAGAAGAAAAATACCGTTGAGGAGGTGGTTAATGAAAACGACGTCGTCGCGGAGGAGGAGGAACGGAAAACAGAGGAGAAAGTTATCGACGGTGGTAATGAACACGAAGAGTATGATTCTCCTGAGAGTGAGATTACTGATTCAGGGTCTCTAGAAAATCAAACTAGTTCGATGAACAGTGATATATGTTCCAATCATGAAGAGTGTGAAGGACGTGCTTCACAGTTTAAATTGACAAAAGGTTTCTCAGCAAAGGAGTCAGTGAAGGGGCATATGGTGAATGTTGTGAAAGGGTTGAAGTTATATGAAGACGGTTTCACTGATTCAGAATTGTGCAAGCTGTCAGATTTTGTGGATGAGATCCATACTGCTGGTCAGAACGGAGAACTGTCTGGTGAAACTTTTATACTATTCAACAAACAGATAAAAGGTAACAAGAGAGAGCTGATTCAGCTGGGTGTTCCAATATTTGGACAGATAAAGGAAGACAACAAAAAATATATAGAGCCAATTCCAGCACTTCTGCAACGTGTCATTGATC
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2532
PRIMER_LEFT_PENALTY=0.128591
PRIMER_RIGHT_PENALTY=0.124655
PRIMER_LEFT_SEQUENCE=AGGAGGTGGTTAATGAAAACGA
PRIMER_RIGHT_SEQUENCE=TCAACCCTTTCACAACATTCAC
PRIMER_LEFT=127,22
PRIMER_RIGHT=405,22
PRIMER_LEFT_TM=59.871
PRIMER_RIGHT_TM=59.875
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.2532
PRIMER_LEFT_1_PENALTY=0.128591
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=GAAAAATACCGTTGAGGAGGTG
PRIMER_RIGHT_1_SEQUENCE=TCAACCCTTTCACAACATTCAC
PRIMER_LEFT_1=113,22
PRIMER_RIGHT_1=405,22
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.2532
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=GAGGAGGTGGTTAATGAAAACG
PRIMER_RIGHT_2_SEQUENCE=TCAACCCTTTCACAACATTCAC
PRIMER_LEFT_2=126,22
PRIMER_RIGHT_2=405,22
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.2995
PRIMER_LEFT_3_PENALTY=0.128591
PRIMER_RIGHT_3_PENALTY=0.170871
PRIMER_LEFT_3_SEQUENCE=GAAAAATACCGTTGAGGAGGTG
PRIMER_RIGHT_3_SEQUENCE=TTCACTGACTCCTTTGCTGAGA
PRIMER_LEFT_3=113,22
PRIMER_RIGHT_3=373,22
PRIMER_LEFT_3_TM=59.871
PRIMER_RIGHT_3_TM=60.171
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.2995
PRIMER_LEFT_4_PENALTY=0.128591
PRIMER_RIGHT_4_PENALTY=0.170871
PRIMER_LEFT_4_SEQUENCE=GAAAAATACCGTTGAGGAGGTG
PRIMER_RIGHT_4_SEQUENCE=TCACTGACTCCTTTGCTGAGAA
PRIMER_LEFT_4=113,22
PRIMER_RIGHT_4=372,22
PRIMER_LEFT_4_TM=59.871
PRIMER_RIGHT_4_TM=60.171
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=260
=
PRIMER_SEQUENCE_ID=SSR_05008 Ps_contig_mira-and-tgicl-ass_franssen_36027
SEQUENCE=GGCGGGGAACTATTTACATTCTAGCATTGTTGAACAAGAAATAAAAAAAGAAGGATTTGCATTGARAAATGAAGAAATCATAGTTGAAGAAAGAAGAAAAAAACAAATATGAATCCATACGACGAAGAGAGGCTAAGAGACGAAGTGATCTATCTACATTCTCTTTGGCACCAAGGTCCTCCTCAACAACCAAACCCTTATCTTCCATTTCCTTCTTCATCCTCCTCACAACCTCTCCTCCATCGCCATCCATTTGCACCCTCCCACACCAGATCCCTTCTCCCCGTCCCTTCAACAGCCTTCAAAGAAAAACCGGAAGAAGAAGAAAGGACCAAAACCCGATTCACCGGCCTGGCCGTGCCTCCATTCATCAGACTCGCCTCAAACAGGTTGGCCGGAACCAAACCCGATCGGATCCCCTCCTCTTAACCGCAAGAGAAAGAGAGTTTTTCTGCGTTACAAGCACAGACCAAGGCTTGCAAAGCTTTTCACGGAATTTCTGAGAA
TARGET=316,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05009 Ps_contig_mira-and-tgicl-ass_franssen_36043
SEQUENCE=GTAGTCAAATTTCCAACATCTTTGGTTGATACTAAACAGAGATGTCATATTTATATATTTATATAAGGCAACAACCCTTTTAATTTCCAACCTTGACTATAAAGAGAGCATTTAAATGAGCAAACTCTCAAACTTAGGAATTAAACAACAAATTTTAACTACAGTTGTTAATAATAATAAGTTTTTAAACCTTATTTCCCAGAAGTTTTAACAAACCCGAGTTCCTTGCGTAGCTGTTCATCAGGTTGCAAGCTATCTGGAATGGCACCAAATATTCTTCGCGATATTTCAAACCCAGAAGCTACATGATGTACATATGCCGATGCATCCTCAACTGTTTGTTGCAAACTTAACGAAGTAGAGAGATAGTTTGAGGTACTTCTTGACAATAATCTTCTATCAGGCGGCTTGTCGAGGTCAGCAGCCTTAAGAAACCGTTCTGTCGCAGCCTTCCATGAAAGGTCATGGATCTGATCATCAGTAAGCTGGTCAGGCTTTTCAGCCAATGCCTTAAGTGTGAGTTGAACAAATTCCTTGTGGTTTGTATACGTCCAACAATTCGGGAACTGCTTGAAAAATTCATTTGAGCAATGGTCTGCGCACACAACGATTTTTCCCATTGCCAAAGCTTCTGCTGTAGTTGTGCAAACCACGTCTGTTGTGCTCGGATTAAGAAACAATTTGAAATCATGAAATAGCTCATCAGCATGATCACGGCCTGGATGAACTCTAACTGTCATTTCCAGCTTGTTAGCAGCTTTTTGAACTTCATCAGAGTCCTCTCCACTTCCAAATAAATCAAGCTCAAAGAGCAGATAATTCCTTTTGATGATCATTAAGAAGTTGGAGCAGCTCTTTGTAGCCTTTGCTCCATATCATTTTCCCAATAAAATAGGCACCTTTGGTAAAAGCATTGTCTTGTATTTTTTTGTTGATTCCCTTTTTTTTTTTGCCAATCT
TARGET=169,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0665
PRIMER_LEFT_PENALTY=0.876929
PRIMER_RIGHT_PENALTY=0.189596
PRIMER_LEFT_SEQUENCE=GGCAACAACCCTTTTAATTTCC
PRIMER_RIGHT_SEQUENCE=GTTTGCAACAAACAGTTGAGGA
PRIMER_LEFT=66,22
PRIMER_RIGHT=348,22
PRIMER_LEFT_TM=60.877
PRIMER_RIGHT_TM=60.190
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=1.0809
PRIMER_LEFT_1_PENALTY=0.876929
PRIMER_RIGHT_1_PENALTY=0.203971
PRIMER_LEFT_1_SEQUENCE=GGCAACAACCCTTTTAATTTCC
PRIMER_RIGHT_1_SEQUENCE=AGAATATTTGGTGCCATTCCAG
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=60.877
PRIMER_RIGHT_1_TM=60.204
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=1.1023
PRIMER_LEFT_2_PENALTY=0.876929
PRIMER_RIGHT_2_PENALTY=0.225403
PRIMER_LEFT_2_SEQUENCE=GGCAACAACCCTTTTAATTTCC
PRIMER_RIGHT_2_SEQUENCE=ATAGCTTGCAACCTGATGAACA
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=60.877
PRIMER_RIGHT_2_TM=59.775
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=1.1304
PRIMER_LEFT_3_PENALTY=0.876929
PRIMER_RIGHT_3_PENALTY=0.253482
PRIMER_LEFT_3_SEQUENCE=GGCAACAACCCTTTTAATTTCC
PRIMER_RIGHT_3_SEQUENCE=TCATGTAGCTTCTGGGTTTGAA
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=308,22
PRIMER_LEFT_3_TM=60.877
PRIMER_RIGHT_3_TM=59.747
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=1.1898
PRIMER_LEFT_4_PENALTY=0.876929
PRIMER_RIGHT_4_PENALTY=0.312895
PRIMER_LEFT_4_SEQUENCE=GGCAACAACCCTTTTAATTTCC
PRIMER_RIGHT_4_SEQUENCE=TTGCAACCTGATGAACAGCTAC
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=250,22
PRIMER_LEFT_4_TM=60.877
PRIMER_RIGHT_4_TM=60.313
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_05010 Ps_contig_mira-and-tgicl-ass_franssen_36047
SEQUENCE=TATTAAAATAAAAATAAACAACGACAGCAATNATGGCAGGGAGAAGAGAATAGACCGAGAGGTGGAAAAAGGAAAAAGAAGAAGACGATAAACTCTCATCAATTCAAGAATAATTGTGTGTAAAGCCATGGAAGAGATGCTGAGTTCATCAACAACAACAATCAACTCTTCCAATATAGGGTTTCAGCTATTGAAGAAGCAGGGTTGGAAAGAAGGCACTGGCCTTGGGATCTCTGAGCAGGGTAGATTAGAACCTGTTGAAACTCATGTGAAGAATAATAAGCGCGGTTTGGGAGCAGATAAAGTGAAGAAAAAGATAGTGAAACCTGATCATGGTGCTGATTCCTCTAAAGGAGACAATAAGCAGGAACATTTGTCTAAAAAGAAAACCAAAGCACTTTCTAAACGGGTGAGAAAGATGCAGGAGTTAGAAAAGAAGATGCAGGAGAAGGAATTTGAGCGCGCTTTCTTTAGAGAGTTCTGGCCTGACAATGTATGAGAATTGGTGATGGGTTCACTAGTGATTTCAGAGTCTGTGCAATAGAGAAATATGCATCACTGGTGATTTCAGAGTCTGTGCAATAGAGAAATATGCACCACTGGTGATTTCAGAGTCTGTGCAAGTTAGNNTCTTAAAGGTTGTTTTTTTGCAAATTGAGGGTAATTTCTGGCAGTTCTTTTTTCATTTAGTAAAATAATCAACTTCTTTTCTTCTTTTCAATTTCTTTTAGTACAATAGAAGAAATAGACTAAAT
TARGET=150,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1682
PRIMER_LEFT_PENALTY=0.168114
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=TGTGTGTAAAGCCATGGAAGAG
PRIMER_RIGHT_SEQUENCE=GCACCATGATCAGGTTTCACTA
PRIMER_LEFT=114,22
PRIMER_RIGHT=338,22
PRIMER_LEFT_TM=60.168
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.2386
PRIMER_LEFT_1_PENALTY=0.151533
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=TGGCAGGGAGAAGAGAATAGAC
PRIMER_RIGHT_1_SEQUENCE=AACCCTGCTTCTTCAATAGCTG
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=59.848
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.2447
PRIMER_LEFT_2_PENALTY=0.157667
PRIMER_RIGHT_2_PENALTY=0.087031
PRIMER_LEFT_2_SEQUENCE=CAGGGAGAAGAGAATAGACCGA
PRIMER_RIGHT_2_SEQUENCE=AACCCTGCTTCTTCAATAGCTG
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=205,22
PRIMER_LEFT_2_TM=59.842
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.2478
PRIMER_LEFT_3_PENALTY=0.247716
PRIMER_RIGHT_3_PENALTY=0.000037
PRIMER_LEFT_3_SEQUENCE=GTAAAGCCATGGAAGAGATGCT
PRIMER_RIGHT_3_SEQUENCE=GCACCATGATCAGGTTTCACTA
PRIMER_LEFT_3=119,22
PRIMER_RIGHT_3=338,22
PRIMER_LEFT_3_TM=59.752
PRIMER_RIGHT_3_TM=60.000
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=0.2872
PRIMER_LEFT_4_PENALTY=0.287182
PRIMER_RIGHT_4_PENALTY=0.000037
PRIMER_LEFT_4_SEQUENCE=GAGAATAGACCGAGAGGTGGAA
PRIMER_RIGHT_4_SEQUENCE=GCACCATGATCAGGTTTCACTA
PRIMER_LEFT_4=45,22
PRIMER_RIGHT_4=338,22
PRIMER_LEFT_4_TM=59.713
PRIMER_RIGHT_4_TM=60.000
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_05011 Ps_contig_mira-and-tgicl-ass_franssen_36048
SEQUENCE=ACATTGACAGGGAAAAGTAAGTTGGGCCGATAAGGTTAACNAAAANTAAATTGAAACAAGTTGTTGTTTCTTGACACTGCATCATCATATAATTGCATTTCATGCACGAATGAACCACCGACACATAAACACCCTCCACCATCACTGTAACATGTTTCCTCATGAATCCTCCAAAACAAAACAAAATATCACCTACTCAGCAACACCTAAGCAAAAACAACACAAGTTTTGAAGATGTTGTGTTGTTTGTTGTTTCTACTAGATGATGATGTTGATATCATGATATGTTTTATGCATTGCATTGAAAAACAAACACCACTATGTAAATTAGAACCAACAACCTCTTACAAATCATTCAACAACATAACTCAGAAGTTTCAATTAGCATGCCACTGAGCATGTAGCAGTAGCTGGTTTTACTTGGAGTAAGACATCAGCGCCGTTTATGGATTGGACGCCGGTAGCGGCTTCTTCTAGACGTTCCCATGTTATTCTTCGTCTCTCGGCTATGTCGCTTGACTTTGAATCTTCCTCTTCGATCTTGCGTTGGCAGGCGAGTACGAGTTCCTCGTCCATCTGAGATAGCATTTTCCTTATGTTCTGAGTTAGGTTCAGCACTGCTTGATTCCAGTGATTCTGTGAGTTATGTACAAGTGCTGAGAAGACGAGAGGCAGAATCACTTGTCGGTTTTGAGTTATAAGATTCAGAATGTGCTCGTTGTTCCATAGCAAATGAGCTCGTTCCGCCACCTGGTAATGTGAGCTAGTGAGGCAGCAAGCCAGTCGTCGAAACAGCGGCACCATAATCTTCTTGAATTCATCCATGCTAGTCATTTCCAAAACCTCTTCTAACTCACTCATGAACATGAGCTCCTTTTGGCTATTTGTAACCGGCCAGTACTTCAATAGTCCTTTTATCACTGAGCTCGCCAATCTTTGATCCTTATCTATGAACTGTACAATGCAATATGTTAACTGCTGATGGTAAATTCCGATAGATTTCGGTTTGTGCAAAGGAATTAAAGCCTTCCACAAAAATATTTTGTGCTCTTCCTTCAACGGCATGGCAAATCCACTAATAACGCTCCCGAAAATCTCCAAGAGCTCGGCAATTCCACTATGCCGCTCGGTCTCAAAAACAAACCGGTAGATAATATTGCTAACCGATTTCCTTATAAACGGCCTATGAACCATGAATTTCCCATAGATTCTATGCAGAACTGTTTTCAAACAATCTCTTTCTCTAGGGTCCTCAGAATCAAAAAGGTCAAGTAATCTTAAAACAAAAGCATGATCTACGTGCGATTTCGCAAGCTTCACATCAAGAGAATTGTAATTGATGAACTGAAGGAGTAGATCATAAACAACTTGCAGGTGAGACCAAGCAGGATCAAACATTGGTTCTTCATCTTCTGTTTCTCCACCGCCACTACTCGAACGAAACTTAGGAGGAAAAACCCTGAACAAATTTGCCGCACACATTTTACACAACGCGGCGATTGCTGGTTCCGTAAACTTAACAGAACCAGATGAAACAAAATCAACAAGGTCTAACAACGTTTGCCGTTTAACATCTTGCTCACTACAGTTCTTATCAGGATCACTCATATCATAAACTTTGCAGCAAAGGTTCAACTTACTGATAAAAAGGCTTTGTTTCTGCGTATTCGAAACATCTTTGAACGATAGAGAAGGATCAACCGCCTCCACTCCGGAATTCACACTTGCAGGAAAAACAACAGAAGATACCCTTTTCACAACATTTAATTTGCTCGAAATTGTACTTCCAACATTCGTGCACTGAAACACGTTCCCAAAACTAGTAGTACTATTGCCAGAATCACTCTTTGCAGAATCTGATGAGTCCGATTTCGGTAACTTCTTAGGCAATTTGCTAAGAATTTGCTTTATCATTGTGGCAATGCTACAAGCCTAGCCTATAACACCTGATTTGTGTGAGGAGTTGAGAAAGTGAAAGAAGACTAGCTAGAGGAAGGTTGATGATGGAAGAAAGAAAGAGAGGATTTTGATGATGAATAATGATTCATGATGAGAATGGATGAATGGTAGTGATGTGAGTAAGCTCAAAGTTTTGGTTCCTCGATTGAAGAGAATAAACGATGGGACATGTTATGNTT
TARGET=172,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2921
PRIMER_LEFT_PENALTY=0.154023
PRIMER_RIGHT_PENALTY=0.138114
PRIMER_LEFT_SEQUENCE=ATGAACCACCGACACATAAACA
PRIMER_RIGHT_SEQUENCE=TCAGTGGCATGCTAATTGAAAC
PRIMER_LEFT=109,22
PRIMER_RIGHT=395,22
PRIMER_LEFT_TM=60.154
PRIMER_RIGHT_TM=60.138
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.4081
PRIMER_LEFT_1_PENALTY=0.269968
PRIMER_RIGHT_1_PENALTY=0.138114
PRIMER_LEFT_1_SEQUENCE=GAATGAACCACCGACACATAAA
PRIMER_RIGHT_1_SEQUENCE=TCAGTGGCATGCTAATTGAAAC
PRIMER_LEFT_1=107,22
PRIMER_RIGHT_1=395,22
PRIMER_LEFT_1_TM=59.730
PRIMER_RIGHT_1_TM=60.138
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=289
PRIMER_PAIR_PENALTY_2=0.4183
PRIMER_LEFT_2_PENALTY=0.154023
PRIMER_RIGHT_2_PENALTY=0.264300
PRIMER_LEFT_2_SEQUENCE=ATGAACCACCGACACATAAACA
PRIMER_RIGHT_2_SEQUENCE=CTCAGTGGCATGCTAATTGAAA
PRIMER_LEFT_2=109,22
PRIMER_RIGHT_2=396,22
PRIMER_LEFT_2_TM=60.154
PRIMER_RIGHT_2_TM=60.264
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.4819
PRIMER_LEFT_3_PENALTY=0.378922
PRIMER_RIGHT_3_PENALTY=0.102945
PRIMER_LEFT_3_SEQUENCE=ATAAACACCCTCCACCATCACT
PRIMER_RIGHT_3_SEQUENCE=ACCAGCTACTGCTACATGCTCA
PRIMER_LEFT_3=124,22
PRIMER_RIGHT_3=414,22
PRIMER_LEFT_3_TM=59.621
PRIMER_RIGHT_3_TM=60.103
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.5059
PRIMER_LEFT_4_PENALTY=0.402961
PRIMER_RIGHT_4_PENALTY=0.102945
PRIMER_LEFT_4_SEQUENCE=ACACATAAACACCCTCCACCAT
PRIMER_RIGHT_4_SEQUENCE=ACCAGCTACTGCTACATGCTCA
PRIMER_LEFT_4=120,22
PRIMER_RIGHT_4=414,22
PRIMER_LEFT_4_TM=60.403
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_05012 Ps_contig_mira-and-tgicl-ass_franssen_36048
SEQUENCE=ACATTGACAGGGAAAAGTAAGTTGGGCCGATAAGGTTAACNAAAANTAAATTGAAACAAGTTGTTGTTTCTTGACACTGCATCATCATATAATTGCATTTCATGCACGAATGAACCACCGACACATAAACACCCTCCACCATCACTGTAACATGTTTCCTCATGAATCCTCCAAAACAAAACAAAATATCACCTACTCAGCAACACCTAAGCAAAAACAACACAAGTTTTGAAGATGTTGTGTTGTTTGTTGTTTCTACTAGATGATGATGTTGATATCATGATATGTTTTATGCATTGCATTGAAAAACAAACACCACTATGTAAATTAGAACCAACAACCTCTTACAAATCATTCAACAACATAACTCAGAAGTTTCAATTAGCATGCCACTGAGCATGTAGCAGTAGCTGGTTTTACTTGGAGTAAGACATCAGCGCCGTTTATGGATTGGACGCCGGTAGCGGCTTCTTCTAGACGTTCCCATGTTATTCTTCGTCTCTCGGCTATGTCGCTTGACTTTGAATCTTCCTCTTCGATCTTGCGTTGGCAGGCGAGTACGAGTTCCTCGTCCATCTGAGATAGCATTTTCCTTATGTTCTGAGTTAGGTTCAGCACTGCTTGATTCCAGTGATTCTGTGAGTTATGTACAAGTGCTGAGAAGACGAGAGGCAGAATCACTTGTCGGTTTTGAGTTATAAGATTCAGAATGTGCTCGTTGTTCCATAGCAAATGAGCTCGTTCCGCCACCTGGTAATGTGAGCTAGTGAGGCAGCAAGCCAGTCGTCGAAACAGCGGCACCATAATCTTCTTGAATTCATCCATGCTAGTCATTTCCAAAACCTCTTCTAACTCACTCATGAACATGAGCTCCTTTTGGCTATTTGTAACCGGCCAGTACTTCAATAGTCCTTTTATCACTGAGCTCGCCAATCTTTGATCCTTATCTATGAACTGTACAATGCAATATGTTAACTGCTGATGGTAAATTCCGATAGATTTCGGTTTGTGCAAAGGAATTAAAGCCTTCCACAAAAATATTTTGTGCTCTTCCTTCAACGGCATGGCAAATCCACTAATAACGCTCCCGAAAATCTCCAAGAGCTCGGCAATTCCACTATGCCGCTCGGTCTCAAAAACAAACCGGTAGATAATATTGCTAACCGATTTCCTTATAAACGGCCTATGAACCATGAATTTCCCATAGATTCTATGCAGAACTGTTTTCAAACAATCTCTTTCTCTAGGGTCCTCAGAATCAAAAAGGTCAAGTAATCTTAAAACAAAAGCATGATCTACGTGCGATTTCGCAAGCTTCACATCAAGAGAATTGTAATTGATGAACTGAAGGAGTAGATCATAAACAACTTGCAGGTGAGACCAAGCAGGATCAAACATTGGTTCTTCATCTTCTGTTTCTCCACCGCCACTACTCGAACGAAACTTAGGAGGAAAAACCCTGAACAAATTTGCCGCACACATTTTACACAACGCGGCGATTGCTGGTTCCGTAAACTTAACAGAACCAGATGAAACAAAATCAACAAGGTCTAACAACGTTTGCCGTTTAACATCTTGCTCACTACAGTTCTTATCAGGATCACTCATATCATAAACTTTGCAGCAAAGGTTCAACTTACTGATAAAAAGGCTTTGTTTCTGCGTATTCGAAACATCTTTGAACGATAGAGAAGGATCAACCGCCTCCACTCCGGAATTCACACTTGCAGGAAAAACAACAGAAGATACCCTTTTCACAACATTTAATTTGCTCGAAATTGTACTTCCAACATTCGTGCACTGAAACACGTTCCCAAAACTAGTAGTACTATTGCCAGAATCACTCTTTGCAGAATCTGATGAGTCCGATTTCGGTAACTTCTTAGGCAATTTGCTAAGAATTTGCTTTATCATTGTGGCAATGCTACAAGCCTAGCCTATAACACCTGATTTGTGTGAGGAGTTGAGAAAGTGAAAGAAGACTAGCTAGAGGAAGGTTGATGATGGAAGAAAGAAAGAGAGGATTTTGATGATGAATAATGATTCATGATGAGAATGGATGAATGGTAGTGATGTGAGTAAGCTCAAAGTTTTGGTTCCTCGATTGAAGAGAATAAACGATGGGACATGTTATGNTT
TARGET=2008,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1700
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.074466
PRIMER_LEFT_SEQUENCE=GCTAGAGGAAGGTTGATGATGG
PRIMER_RIGHT_SEQUENCE=ATTCTCTTCAATCGAGGAACCA
PRIMER_LEFT=1985,22
PRIMER_RIGHT=2114,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=0.2230
PRIMER_LEFT_1_PENALTY=0.148509
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=CCTGATTTGTGTGAGGAGTTGA
PRIMER_RIGHT_1_SEQUENCE=ATTCTCTTCAATCGAGGAACCA
PRIMER_LEFT_1=1945,22
PRIMER_RIGHT_1=2114,22
PRIMER_LEFT_1_TM=60.149
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.4642
PRIMER_LEFT_2_PENALTY=0.095574
PRIMER_RIGHT_2_PENALTY=0.368624
PRIMER_LEFT_2_SEQUENCE=GCTAGAGGAAGGTTGATGATGG
PRIMER_RIGHT_2_SEQUENCE=CTTCAATCGAGGAACCAAAACT
PRIMER_LEFT_2=1985,22
PRIMER_RIGHT_2=2109,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=59.631
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.5171
PRIMER_LEFT_3_PENALTY=0.148509
PRIMER_RIGHT_3_PENALTY=0.368624
PRIMER_LEFT_3_SEQUENCE=CCTGATTTGTGTGAGGAGTTGA
PRIMER_RIGHT_3_SEQUENCE=CTTCAATCGAGGAACCAAAACT
PRIMER_LEFT_3=1945,22
PRIMER_RIGHT_3=2109,22
PRIMER_LEFT_3_TM=60.149
PRIMER_RIGHT_3_TM=59.631
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.5572
PRIMER_LEFT_4_PENALTY=0.482784
PRIMER_RIGHT_4_PENALTY=0.074466
PRIMER_LEFT_4_SEQUENCE=TGAGTCCGATTTCGGTAACTTC
PRIMER_RIGHT_4_SEQUENCE=ATTCTCTTCAATCGAGGAACCA
PRIMER_LEFT_4=1860,22
PRIMER_RIGHT_4=2114,22
PRIMER_LEFT_4_TM=60.483
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_05013 Ps_contig_mira-and-tgicl-ass_franssen_36050
SEQUENCE=TTTCACATGCACGACACCATCACAGACGAAGACGGAGATGTAACTGAGGACAGCGAGCCGGAGAATCACAACGAAGAAGAAGAAGAGCAGCAAGCAGTGAATTATAGAAGCTCAATCACTTCTGTAGGTATTAGTCGAGTTGTGGACCCGAACGAGCGAAATAGGGTGGTAAGAATGGAACAGAGTGTTCTTGCTAGTATGCGGAGCAGGGTTGTTTGTGGATCCACTGTTCTTCTACGCCCTTTCCATCAGCGACTCATTTATGTGCCTCTTCATCGACGGCTGGTTCGCCGTCACCGTAACGGTGCTCCGTTGCATGACTGACGCTTTACACCTTTGGCATATCTTCCTCCGCTTTAAGATGGCAAAACGCAACTCATTCGTAATCTCCGGGGATACTGCGGGAGCTGATGCCCGATGCTCGGTGGCTCTTGCTTATCTTAAGTCCCGCCGCGGCTTCTTCTTTGATCTCTTCGTTATTCTTCCTATACCTCAGATTGTGCTTTGGGTGGCAATCCCTTCTCTGTTNGGAGCAAGGTTCAGTTACGTTGGTGATGACAATATTATTAATTGTATTTCTGTTCCAATACCTTCCCAAAATTTATCACTCAGTATGCCATCTTAGACGCACACAAAACCTGTCTGGCTACATTTTTGGAACTGTTTGGTGGGGTATTGCTCTTAACATGATCGCCTATTTTGTAGCTTCCCATGCTGCAGGCGCATGTTGGTACTTGTTGGGGATCCAAAGAGCAACGAAATGTCTGAAAGAGCAATGTCTGAAAACAAATGGTTGTGGCATAAAAATATTGTCTTGTCATAAGCCAATATATTATGGAAGTACAAGTTTGTTGAATAAAGAAATTAGAAGAATGTGGTGGGCAGATAATAGGGAAGCAAGGTCTACATGCATAAATGGTCCAGATAACTTCAACTATGGAGCTTATAAATGGACTGTTCAACTTGTCACTAATGATAACAGACTTGAAAAGATACTTTTTCCTATCTTTTGGGGCCTAATGACTCTCAGCACTTTCGGGAACCTAGAAAGCACAACGGACTGGCTAGAAGTAGTTTTCAATATCATAGTCTTGACTAGTGGCCTTCTTCTTGTGACTATGCTGATTGGAAACATCAAGGTGTTTTTGCATGCAACAACATCAAAGAAACAAGTGATGCAATTGAAGATGGGAAATGTTGAATGGTGGATGAGGAAAAAACCATTTGCCGCAGGGGTTTCGACAACGTGTGCGTAACTATGAAAGACAGCGCTGGGCTGCTATGCGTGGGGTTGATGAATGTAGAAATAGAACCAAAATCTTCCTAGTAAGGTTAAGAAGAGA
TARGET=73,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4081
PRIMER_LEFT_PENALTY=0.312496
PRIMER_RIGHT_PENALTY=0.095629
PRIMER_LEFT_SEQUENCE=CACAGACGAAGACGGAGATGTA
PRIMER_RIGHT_SEQUENCE=CATAAATGAGTCGCTGATGGAA
PRIMER_LEFT=20,22
PRIMER_RIGHT=264,22
PRIMER_LEFT_TM=60.312
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=245
PRIMER_PAIR_PENALTY_1=0.4081
PRIMER_LEFT_1_PENALTY=0.312496
PRIMER_RIGHT_1_PENALTY=0.095629
PRIMER_LEFT_1_SEQUENCE=CACAGACGAAGACGGAGATGTA
PRIMER_RIGHT_1_SEQUENCE=CGATGAAGAGGCACATAAATGA
PRIMER_LEFT_1=20,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=60.312
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.4485
PRIMER_LEFT_2_PENALTY=0.312496
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=CACAGACGAAGACGGAGATGTA
PRIMER_RIGHT_2_SEQUENCE=TCTGTTCCATTCTTACCACCCT
PRIMER_LEFT_2=20,22
PRIMER_RIGHT_2=183,22
PRIMER_LEFT_2_TM=60.312
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.4485
PRIMER_LEFT_3_PENALTY=0.312496
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CACAGACGAAGACGGAGATGTA
PRIMER_RIGHT_3_SEQUENCE=CTCTGTTCCATTCTTACCACCC
PRIMER_LEFT_3=20,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=60.312
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.4486
PRIMER_LEFT_4_PENALTY=0.312496
PRIMER_RIGHT_4_PENALTY=0.136083
PRIMER_LEFT_4_SEQUENCE=CACAGACGAAGACGGAGATGTA
PRIMER_RIGHT_4_SEQUENCE=CATTCTTACCACCCTATTTCGC
PRIMER_LEFT_4=20,22
PRIMER_RIGHT_4=176,22
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_05014 Ps_contig_mira-and-tgicl-ass_franssen_36052
SEQUENCE=ACACTCTGTTATCTCTGTTATTGTTTCCAAGAAAAATAACAATAAAAGAAATGGAACGGCGGCAACAGTCACCACCATCTCCGCCTCCATCTCCGCCGTCACGAATCATACTTGGAAAATACCAGCTTACCAAATTTCTGGGACGCGGAAACTTCGCGAAGGTATACCAAGCAACTTCTCTGATAGATGGTTCAACGGTAGCCGTGAAAGTCATTGACAAATCCAAAACGGTGGACGCATCTATGGAGCCACGAATAGTTCGTGAAATCGACGCTATGCGCCGTCTTCAAAATCATCAAAATATCCTCAAAATTCATGAAGTTTTAGCTACGAAAACAAAGATCTATCTCATCGTCGATTTCGCCGGCGGCGGTGAGCTTTTCTACAAACTCTCCCGACGAGGCAGGTTTACAGAGTCTATTGCTCGTCGTTACTTTCAACAGCTTGTGTCCGCACTCTGTTTCTGTCACAAAACGGCGTCGCTCACCGTGACCTAAAACCGCAGAATCTTCTCCTCGACGCTGACGGTAATCTAAAGGTCTCCGACTTCGGTCTCTCCGCCTTGCCGGAACAGCTCCAGAACGGTCTCCTCCATACAGCTTGTGGTACTCCGGCGTACACCGCACCGGAGATTCTCGGCCGCATTGGATACGACGGATCTAAAGCAGACGCTTGGTCCTGTGGCGTCATCCTCTACGTGCTTCTCGCTGGTCACCTTCCGTTCGACGATTCAAACATTCCGGCGATGTGCAAGAGAATCATGCGCCGGGATTTTCAGTTTCCGGACTGGATTTCTAAGCCAGCTCGTTACCTAATCTATCAGCTACTCGACCCGAACCCGAAAACCCGAATCAAACTAGAAAACGTGTTTGGAAACGCGTGGTTTAAGAAATCATTAAGAGAAGAACCTGAAGTTAAAGTTTTCGAATCGGAATTATACAACAAATACTGTTGCGAAGGTAACAAGAAATTAGAATTGGGGATGAACGCGTTTGATATAATATCGATGTCTTCGGGTTTGGATTTGAGTGGTTTGTTTGAAACTACATCGTTTAGAACTGAAAAGAGGTTTACTTCAAGTGAAGAAATTGGTGTGGTGGAAGAGAAAGTGAAGGAAATTGGAGGAGAATTAGGGTTTAGAGTTGAGATTGGGAAAAATGGAACAATTGGGTTGGGGAAAGGAAAGATTACTATGGTTGTTGAGGTGTTTAAGATTGTGGACAATTTGTTGTTGGTAGCACTTAAATTGGAGAATGGTGGAATGGAATTTGAAGAACTTCATTGGAATGATTGGAAAATTGGGCTTCAAGATGTTGTTATTTCTTGGCATAATTACGAGTCAAAATTTCCATGTAATAATAATGTTGATGAATCTGAAAGAAAGATTACTTAGTTCCGATGTAAAATATGTTAGGAGAGATTAACCCTTTCAAAACAGTTACCTCTGTTTTGTTATTCACTCTCACACAAACAAACAAGTGTAGGGTTCTCATGCATGTAGCTTCTAATATACACTTTTTTGTTTGTTTGATTCTTAGTTGTTAATGAGTGAATTTCTTTTGTTTGTT
TARGET=1467,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2538
PRIMER_LEFT_PENALTY=0.095538
PRIMER_RIGHT_PENALTY=0.158297
PRIMER_LEFT_SEQUENCE=TGGACAATTTGTTGTTGGTAGC
PRIMER_RIGHT_SEQUENCE=AAGCTACATGCATGAGAACCCT
PRIMER_LEFT=1217,22
PRIMER_RIGHT=1503,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=60.158
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.4706
PRIMER_LEFT_1_PENALTY=0.312329
PRIMER_RIGHT_1_PENALTY=0.158297
PRIMER_LEFT_1_SEQUENCE=GGTGGAATGGAATTTGAAGAAC
PRIMER_RIGHT_1_SEQUENCE=AAGCTACATGCATGAGAACCCT
PRIMER_LEFT_1=1255,22
PRIMER_RIGHT_1=1503,22
PRIMER_LEFT_1_TM=59.688
PRIMER_RIGHT_1_TM=60.158
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=0.7006
PRIMER_LEFT_2_PENALTY=0.542262
PRIMER_RIGHT_2_PENALTY=0.158297
PRIMER_LEFT_2_SEQUENCE=GAGAATGGTGGAATGGAATTTG
PRIMER_RIGHT_2_SEQUENCE=AAGCTACATGCATGAGAACCCT
PRIMER_LEFT_2=1249,22
PRIMER_RIGHT_2=1503,22
PRIMER_LEFT_2_TM=60.542
PRIMER_RIGHT_2_TM=60.158
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=1.1121
PRIMER_LEFT_3_PENALTY=0.095538
PRIMER_RIGHT_3_PENALTY=1.016543
PRIMER_LEFT_3_SEQUENCE=TGGACAATTTGTTGTTGGTAGC
PRIMER_RIGHT_3_SEQUENCE=GAAGCTACATGCATGAGAACCC
PRIMER_LEFT_3=1217,22
PRIMER_RIGHT_3=1504,22
PRIMER_LEFT_3_TM=59.904
PRIMER_RIGHT_3_TM=61.017
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=1.2108
PRIMER_LEFT_4_PENALTY=0.095538
PRIMER_RIGHT_4_PENALTY=1.115242
PRIMER_LEFT_4_SEQUENCE=TGGACAATTTGTTGTTGGTAGC
PRIMER_RIGHT_4_SEQUENCE=AGCTACATGCATGAGAACCCTA
PRIMER_LEFT_4=1217,22
PRIMER_RIGHT_4=1502,22
PRIMER_LEFT_4_TM=59.904
PRIMER_RIGHT_4_TM=58.885
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_05015 Ps_contig_mira-and-tgicl-ass_franssen_36052
SEQUENCE=ACACTCTGTTATCTCTGTTATTGTTTCCAAGAAAAATAACAATAAAAGAAATGGAACGGCGGCAACAGTCACCACCATCTCCGCCTCCATCTCCGCCGTCACGAATCATACTTGGAAAATACCAGCTTACCAAATTTCTGGGACGCGGAAACTTCGCGAAGGTATACCAAGCAACTTCTCTGATAGATGGTTCAACGGTAGCCGTGAAAGTCATTGACAAATCCAAAACGGTGGACGCATCTATGGAGCCACGAATAGTTCGTGAAATCGACGCTATGCGCCGTCTTCAAAATCATCAAAATATCCTCAAAATTCATGAAGTTTTAGCTACGAAAACAAAGATCTATCTCATCGTCGATTTCGCCGGCGGCGGTGAGCTTTTCTACAAACTCTCCCGACGAGGCAGGTTTACAGAGTCTATTGCTCGTCGTTACTTTCAACAGCTTGTGTCCGCACTCTGTTTCTGTCACAAAACGGCGTCGCTCACCGTGACCTAAAACCGCAGAATCTTCTCCTCGACGCTGACGGTAATCTAAAGGTCTCCGACTTCGGTCTCTCCGCCTTGCCGGAACAGCTCCAGAACGGTCTCCTCCATACAGCTTGTGGTACTCCGGCGTACACCGCACCGGAGATTCTCGGCCGCATTGGATACGACGGATCTAAAGCAGACGCTTGGTCCTGTGGCGTCATCCTCTACGTGCTTCTCGCTGGTCACCTTCCGTTCGACGATTCAAACATTCCGGCGATGTGCAAGAGAATCATGCGCCGGGATTTTCAGTTTCCGGACTGGATTTCTAAGCCAGCTCGTTACCTAATCTATCAGCTACTCGACCCGAACCCGAAAACCCGAATCAAACTAGAAAACGTGTTTGGAAACGCGTGGTTTAAGAAATCATTAAGAGAAGAACCTGAAGTTAAAGTTTTCGAATCGGAATTATACAACAAATACTGTTGCGAAGGTAACAAGAAATTAGAATTGGGGATGAACGCGTTTGATATAATATCGATGTCTTCGGGTTTGGATTTGAGTGGTTTGTTTGAAACTACATCGTTTAGAACTGAAAAGAGGTTTACTTCAAGTGAAGAAATTGGTGTGGTGGAAGAGAAAGTGAAGGAAATTGGAGGAGAATTAGGGTTTAGAGTTGAGATTGGGAAAAATGGAACAATTGGGTTGGGGAAAGGAAAGATTACTATGGTTGTTGAGGTGTTTAAGATTGTGGACAATTTGTTGTTGGTAGCACTTAAATTGGAGAATGGTGGAATGGAATTTGAAGAACTTCATTGGAATGATTGGAAAATTGGGCTTCAAGATGTTGTTATTTCTTGGCATAATTACGAGTCAAAATTTCCATGTAATAATAATGTTGATGAATCTGAAAGAAAGATTACTTAGTTCCGATGTAAAATATGTTAGGAGAGATTAACCCTTTCAAAACAGTTACCTCTGTTTTGTTATTCACTCTCACACAAACAAACAAGTGTAGGGTTCTCATGCATGTAGCTTCTAATATACACTTTTTTGTTTGTTTGATTCTTAGTTGTTAATGAGTGAATTTCTTTTGTTTGTT
TARGET=1519,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05016 Ps_contig_mira-and-tgicl-ass_franssen_36071
SEQUENCE=TCGGAATGAGAGAAGATCATAGCGAGAGAGATGCAAACGACGCTGTTTCGACGGCTCGCTCCATCGTTGGCAGCACGATATCAACGCAACGAGAATCTTCTAACCTCTTTCTCTTCTTTATCTTTTTCTTCTTCTTCTTCGGCTTCTTCGGTTCTTCACCATGCCACGTCTTCGTCTTCTTCTTCTGCTGACGTGGAACCTGTTCACATAACCGAAAACTGCGTTCGAAGAATTAAGGAACTGGATTCTAATGAACCATCATCTGCTGGAAAAATGCTTCGTTTGAGTGTAGAAACTGGCGGATGTTCGGGATTCCAGTATGCTTTTAATCTGGAAGACAGAGTCAACTCGGACGATAGAGTTTTCGAGAAGGAAGGAATTAAATTGGTTGTTGACAATATTTCATACGACTTTGTTAAAGGCGCGACTGTTGATTATGTCGAGGAGCTAATCCGTTCGGCTTTCGTAGTGACTGAGAACCCCAGTGCAGTTGGCGGTTGTAGCTGTAAAAGTTCCTTCATGGTGAAACAGTAGTTAGCTATCTTGGTACTCCCCTCCTCAACTTCCTCCACTGCTCAGATATATACTCAATGAACAAATATTATGAACATGATTTTTCTAACTCAAGAAACAATTGATTAAATTATTGTTTGTATAATGATATCAATGTAACATACAGTTGGTGTATTGGTAATAAGTTAGCAGGCATTTCAGTCTAGCCTGTTCTAATGTCTTTTGGTGTATATTAAATGAAGCCTGCAAATGTATTTACAGTTTGCAGCAACAAGTTGCTTGTTTGATGCCATTTAATGACAATGACTTGGTGGTAAACCTTGCTAGAGCTGGTGATGCGGGCTGTTGGTCCGCCCTGTTATAACCAGTGCCACAATAAATCTGCATACTCCCTCCAAA
TARGET=126,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3830
PRIMER_LEFT_PENALTY=0.087031
PRIMER_RIGHT_PENALTY=0.295944
PRIMER_LEFT_SEQUENCE=CGCAACGAGAATCTTCTAACCT
PRIMER_RIGHT_SEQUENCE=GTCCGAGTTGACTCTGTCTTCC
PRIMER_LEFT=84,22
PRIMER_RIGHT=353,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=60.296
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=270
PRIMER_PAIR_PENALTY_1=0.4466
PRIMER_LEFT_1_PENALTY=0.087031
PRIMER_RIGHT_1_PENALTY=0.359557
PRIMER_LEFT_1_SEQUENCE=CGCAACGAGAATCTTCTAACCT
PRIMER_RIGHT_1_SEQUENCE=CGAGTTGACTCTGTCTTCCAGA
PRIMER_LEFT_1=84,22
PRIMER_RIGHT_1=350,22
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=59.640
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.4717
PRIMER_LEFT_2_PENALTY=0.087031
PRIMER_RIGHT_2_PENALTY=0.384647
PRIMER_LEFT_2_SEQUENCE=CGCAACGAGAATCTTCTAACCT
PRIMER_RIGHT_2_SEQUENCE=CTCGAAAACTCTATCGTCCGAG
PRIMER_LEFT_2=84,22
PRIMER_RIGHT_2=368,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=60.385
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.4952
PRIMER_LEFT_3_PENALTY=0.087031
PRIMER_RIGHT_3_PENALTY=0.408180
PRIMER_LEFT_3_SEQUENCE=CGCAACGAGAATCTTCTAACCT
PRIMER_RIGHT_3_SEQUENCE=CCAGCAGATGATGGTTCATTAG
PRIMER_LEFT_3=84,22
PRIMER_RIGHT_3=268,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=59.592
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=0.5023
PRIMER_LEFT_4_PENALTY=0.087031
PRIMER_RIGHT_4_PENALTY=0.415247
PRIMER_LEFT_4_SEQUENCE=CGCAACGAGAATCTTCTAACCT
PRIMER_RIGHT_4_SEQUENCE=GAAGAAGAAGACGAAGACGTGG
PRIMER_LEFT_4=84,22
PRIMER_RIGHT_4=184,22
PRIMER_LEFT_4_TM=59.913
PRIMER_RIGHT_4_TM=60.415
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_05017 Ps_contig_mira-and-tgicl-ass_franssen_36071
SEQUENCE=TCGGAATGAGAGAAGATCATAGCGAGAGAGATGCAAACGACGCTGTTTCGACGGCTCGCTCCATCGTTGGCAGCACGATATCAACGCAACGAGAATCTTCTAACCTCTTTCTCTTCTTTATCTTTTTCTTCTTCTTCTTCGGCTTCTTCGGTTCTTCACCATGCCACGTCTTCGTCTTCTTCTTCTGCTGACGTGGAACCTGTTCACATAACCGAAAACTGCGTTCGAAGAATTAAGGAACTGGATTCTAATGAACCATCATCTGCTGGAAAAATGCTTCGTTTGAGTGTAGAAACTGGCGGATGTTCGGGATTCCAGTATGCTTTTAATCTGGAAGACAGAGTCAACTCGGACGATAGAGTTTTCGAGAAGGAAGGAATTAAATTGGTTGTTGACAATATTTCATACGACTTTGTTAAAGGCGCGACTGTTGATTATGTCGAGGAGCTAATCCGTTCGGCTTTCGTAGTGACTGAGAACCCCAGTGCAGTTGGCGGTTGTAGCTGTAAAAGTTCCTTCATGGTGAAACAGTAGTTAGCTATCTTGGTACTCCCCTCCTCAACTTCCTCCACTGCTCAGATATATACTCAATGAACAAATATTATGAACATGATTTTTCTAACTCAAGAAACAATTGATTAAATTATTGTTTGTATAATGATATCAATGTAACATACAGTTGGTGTATTGGTAATAAGTTAGCAGGCATTTCAGTCTAGCCTGTTCTAATGTCTTTTGGTGTATATTAAATGAAGCCTGCAAATGTATTTACAGTTTGCAGCAACAAGTTGCTTGTTTGATGCCATTTAATGACAATGACTTGGTGGTAAACCTTGCTAGAGCTGGTGATGCGGGCTGTTGGTCCGCCCTGTTATAACCAGTGCCACAATAAATCTGCATACTCCCTCCAAA
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3830
PRIMER_LEFT_PENALTY=0.087031
PRIMER_RIGHT_PENALTY=0.295944
PRIMER_LEFT_SEQUENCE=CGCAACGAGAATCTTCTAACCT
PRIMER_RIGHT_SEQUENCE=GTCCGAGTTGACTCTGTCTTCC
PRIMER_LEFT=84,22
PRIMER_RIGHT=353,22
PRIMER_LEFT_TM=59.913
PRIMER_RIGHT_TM=60.296
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=270
PRIMER_PAIR_PENALTY_1=0.4466
PRIMER_LEFT_1_PENALTY=0.087031
PRIMER_RIGHT_1_PENALTY=0.359557
PRIMER_LEFT_1_SEQUENCE=CGCAACGAGAATCTTCTAACCT
PRIMER_RIGHT_1_SEQUENCE=CGAGTTGACTCTGTCTTCCAGA
PRIMER_LEFT_1=84,22
PRIMER_RIGHT_1=350,22
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=59.640
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.4717
PRIMER_LEFT_2_PENALTY=0.087031
PRIMER_RIGHT_2_PENALTY=0.384647
PRIMER_LEFT_2_SEQUENCE=CGCAACGAGAATCTTCTAACCT
PRIMER_RIGHT_2_SEQUENCE=CTCGAAAACTCTATCGTCCGAG
PRIMER_LEFT_2=84,22
PRIMER_RIGHT_2=368,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=60.385
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.4952
PRIMER_LEFT_3_PENALTY=0.087031
PRIMER_RIGHT_3_PENALTY=0.408180
PRIMER_LEFT_3_SEQUENCE=CGCAACGAGAATCTTCTAACCT
PRIMER_RIGHT_3_SEQUENCE=CCAGCAGATGATGGTTCATTAG
PRIMER_LEFT_3=84,22
PRIMER_RIGHT_3=268,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=59.592
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=0.5068
PRIMER_LEFT_4_PENALTY=0.210886
PRIMER_RIGHT_4_PENALTY=0.295944
PRIMER_LEFT_4_SEQUENCE=ACGCAACGAGAATCTTCTAACC
PRIMER_RIGHT_4_SEQUENCE=GTCCGAGTTGACTCTGTCTTCC
PRIMER_LEFT_4=83,22
PRIMER_RIGHT_4=353,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=60.296
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_05018 Ps_contig_mira-and-tgicl-ass_franssen_36074
SEQUENCE=AAAACAAAACTAAAATACATATCATATATATAATAAAGTATACCTTTGTCAACTACAATGATCCAAGAAATACTGAACATAGTGATTTACACATGATTTATTTACATTCAAAATATACAGCCTTGACAAATAGCATAAGAGAAGTTGAGTACATTATATACATATCCTATCTCTCCATGACATAGATGCATCACTTCTAATTTTGCTACATAATGTTCATCCCAATAAGTTGTGTCTTGCAAAAGGGAGAGACTGACAGACAAAAAAAAGGTTTTCTACATCACCATATCAAGGGCCATCCACTATTTTGGTGTCAAATATTCTAAAGTTTCTACTTCCCCATACATCACTTTTTGAGACATAGTATGATTTAATACAAACACTCACCCATGCTGGATTTCTTGGCCTATCATTTACCACAATACTGTCATCACATTCAGTTACTCCAAGTAATCTGTTCAACTTCCGTTTCACATTTGCAGTGCCGGGATAACCATCAAAAAGAGTCTCCCCATCTTCATCAACTACAAAAGCATGGATTCTAGCTGTTGGATCCTCTAGGGTCAGTCTCATCATATACTTCCCAGCAGGCGTACAGAACTTTTCACCTTGGTACGGTACTGCTGCTACCACTCGAACAACACACTTAAACTTAGCTGTCACCTCTGAATGAGTGAGTACATCCATTAAAGTAAGATGCCGCACATGATCGTGATTAACCTTTGTAATACATAAAGACTCGGGTAAGCTGTCAATGGACTGAGGCATGCTACCAATAGGCATGTTTCTTGATTTCAACGATATGCGGTCATCATACAACCTTTGACGTTCTGCTATGAGACGGTCTTCATTTGGTGTATGTCGAAGCTTTGAATGTGAGGTAAACACACCATACCACAGCCCTGCATACACTTTGAGACGTATGTTGATAAACTGGGCCCATTTACCACTTTTAAGAACATTGAGATGATTTTTCTCTATGGGTAGTTCAAAAGCAATCCTCAAGATGGAGCCAACAGTAGGCAGAGTACATAGTACATCTCTTGGCAAAGGCAGAGATTCCAATTGTAATGGAAGTGGATGATTAATTTCATCTTCCAGCATTGAAGTTATGGCATTTGGCGGGGTATCTGTACCGTCCCAAACATAGACAAACCAATTATCTTTCAAAGCCTCCCAGCAATGAAGTATCTTGCCGGCCAAATTAAAGTGACCTTCTTTGATTTCTCTTAGCATAGGAAAATCACTTGACTCTGCAGGAATCTGAAAATTGACCAACCATTTTCGAAGCATGTTTATGCGCATTTTGTCCTCGTCTCTGAGGAAAACTTTTGAATAAACTTGGTAAGGGACAAAATCATCATCATCATCACCATTCCCTCTATATAAGGCAAAAGAGGAAGAGTTTTTGTAGAAAACAGGCATTTACYTCCTTAATATGCGTCCTTAACGTAACATTA
TARGET=1357,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05019 Ps_contig_mira-and-tgicl-ass_franssen_36077
SEQUENCE=TCAACATAAGCCGGATATGGATCAAAGTCGGAATGTGGTGCCCCATTCTTGATTAGTCTGCGCACCATTTTGATTTCATCTTTTGTGAGCTCCACTACCTCATCATTGTAATCATCATAGATCTTCCGCCAATTCTTAGAATCATCAACATTTTTAAGAAAGGAATCCAATTTGTCTGCCTTTTCTTTCTTCTTAATCTTCTTTCCCGTAATGTCATATCCAATATGGGGTTCATCGGCATACCACTTCAAAGGAACCTCTCCAATAGTATTTCGGGGAGCCACCTCATCCTCGGATGAATCACTCTCAACTCCTCCTCCCTGATGAAGATCAGAGCTCTCACTTCCACCTTCTGAGTTGACATCATCCTCGTCTTGAGATTCTGCATCCTCTGAATCACCGGAGTTATCATCATCTTCTTCTTCTAATTCACCAGAACCAGCAGATTCGGATTGAGAGTCCAGTGATTCGGAAGAGTCCTCGTCGCTGTCTCCAGAAAAGTACTCGTAATCAGAGTCATCGGAATCGGATGGAACTGGTGCTTCCTTCTGATTCTCGACCTCCTCGTCTTTTTGGTCCATCGTCGTCGATTTCTTCACCTCCGTGGATTTGGGCGTTGCTGATTTCTTTCCCTGCCCCTTCATGGAATGGGTGTGGCGGAGTTGCTGTTGATTAGGGTTTTGGTGTTTTGTATTTTTCTTTTACAAGGTTAGGGTTCTAGGGTTTTAGCGTATAGTTTCAGGCTTTCCCGAC
TARGET=415,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2376
PRIMER_LEFT_PENALTY=0.115965
PRIMER_RIGHT_PENALTY=0.121644
PRIMER_LEFT_SEQUENCE=TCACTTCCACCTTCTGAGTTGA
PRIMER_RIGHT_SEQUENCE=CTCTTCCGAATCACTGGACTCT
PRIMER_LEFT=339,22
PRIMER_RIGHT=476,22
PRIMER_LEFT_TM=59.884
PRIMER_RIGHT_TM=59.878
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=138
PRIMER_PAIR_PENALTY_1=0.2404
PRIMER_LEFT_1_PENALTY=0.118777
PRIMER_RIGHT_1_PENALTY=0.121644
PRIMER_LEFT_1_SEQUENCE=GTTGACATCATCCTCGTCTTGA
PRIMER_RIGHT_1_SEQUENCE=CTCTTCCGAATCACTGGACTCT
PRIMER_LEFT_1=356,22
PRIMER_RIGHT_1=476,22
PRIMER_LEFT_1_TM=60.119
PRIMER_RIGHT_1_TM=59.878
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=121
PRIMER_PAIR_PENALTY_2=0.2814
PRIMER_LEFT_2_PENALTY=0.038361
PRIMER_RIGHT_2_PENALTY=0.243031
PRIMER_LEFT_2_SEQUENCE=ATCCTCTGAATCACCGGAGTTA
PRIMER_RIGHT_2_SEQUENCE=AATCAGAAGGAAGCACCAGTTC
PRIMER_LEFT_2=386,22
PRIMER_RIGHT_2=553,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.2814
PRIMER_LEFT_3_PENALTY=0.038361
PRIMER_RIGHT_3_PENALTY=0.243031
PRIMER_LEFT_3_SEQUENCE=ATCCTCTGAATCACCGGAGTTA
PRIMER_RIGHT_3_SEQUENCE=GAATCAGAAGGAAGCACCAGTT
PRIMER_LEFT_3=386,22
PRIMER_RIGHT_3=554,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=59.757
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=0.2814
PRIMER_LEFT_4_PENALTY=0.038361
PRIMER_RIGHT_4_PENALTY=0.243031
PRIMER_LEFT_4_SEQUENCE=TCCTCTGAATCACCGGAGTTAT
PRIMER_RIGHT_4_SEQUENCE=AATCAGAAGGAAGCACCAGTTC
PRIMER_LEFT_4=387,22
PRIMER_RIGHT_4=553,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=59.757
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_05020 Ps_contig_mira-and-tgicl-ass_franssen_36083
SEQUENCE=TTGAAATTAGTCCCTCCCTGCTTGTTATATGGNTATCCATTTCATCTAGTGGATGTGCTTTCTTTTGTTTCTTTAGTGATTTTTTTTTTTGGATACTTGGTAGAACTTGTTTTCTTTAAATAAGGAGATTTATTTATATTTGTTTTTGGTGTTGAATTAATAGGTTTAACATAAAATACACTATCAAAAGTATGTGTTTTAGCAGATTTGAACATTTAGAAGAGTGAAATGTGAAAGGCTTTAATCAATATTCTAAATACTGCATTGGCTTGTTCAACCGAGAACTGATTGTGCTTGTAAAAATTAAAAGAAGTAATAATAATAA
TARGET=314,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_05021 Ps_contig_mira-and-tgicl-ass_franssen_36090
SEQUENCE=ACAAGAGTATCCATGCCGCCAGAAGCGACGCCGCCCTGAGGCAAATCGGAATTTGGAGGAATCCATGTCAACGGACCGACGAAGCTGGTGTGACCTACCAGAAGTTTGGAAGAGACGAATTTGCGATTTTCCTGAGACCATAACCTAACGGTTTGTCCCTGGATGATGTGGCTATGTCGCCGTTGCCGCAGATGGTTATTCCACGAACGTCGTCTTCGTGGCCGTGAAGTTCACAGCGTAACTGATACTCTTTGAAATCAATGTCCTATTTTTTTGTTTGAGAGAGAGAGAGAAAGGA
TARGET=280,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05022 Ps_contig_mira-and-tgicl-ass_franssen_36094
SEQUENCE=ATTNATAGAAAGAGAAAATAAACAAGTGGAGGAAGGTGTGAGCAGTGATGTTTGTTTGTGGAGCAAAATGTGTCTCTCACTCTCCTCCTCTTCCATTTCCATCCAGATGCTTAAACTCAAACCTTTCTTCTTCTTCCAACAACTACCGACCCGCTGTTATTCTCCCGGGATTAGGGAACAATTCAGGTGACTACCAGAAACTGGAGGCAACGCTGAACGATAAGTACGGCGTGTCCACTGTGGTGGCCAAGGTATCGAGACTTGACTGGTTCCGAAATGCTGCTGGGTTGATTGATCCCAATTACTGGCGCGGCACTCTTCAACCAAGCCCTGTCCTCGATTGGTATTTGAAAAGAGTTGATGATGCTGTCGAAGAGGCGAAGGAGTTAGCACCACCAGGTTCCACTTTATCGTTGATTGGCCACTCAGCAGGAGGATGGCTTGCACGCCTCTATATGGAACAATTTGGAGTCTCCAATATATCCTTGTTATTAACTCTCGGTACCCCTCACCTGCCAGCTCCAAAAGGTGTCTCTGGTGTTATAGATCAAACCAGGGGTCTTCTTGATTATGTTGAACAATATTGCTCTAAACCTGTTTACACTCCGGAACTCAAATATAGTAATAGTAATAAGACAAGGAAAAGGTATATTGAAGGGGCTCCACTTTTTGGCANTT
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1058
PRIMER_LEFT_PENALTY=0.074695
PRIMER_RIGHT_PENALTY=0.031122
PRIMER_LEFT_SEQUENCE=ACTCTCCTCCTCTTCCATTTCC
PRIMER_RIGHT_SEQUENCE=CCTCCAGTTTCTGGTAGTCACC
PRIMER_LEFT=78,22
PRIMER_RIGHT=206,22
PRIMER_LEFT_TM=60.075
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=129
PRIMER_PAIR_PENALTY_1=0.1119
PRIMER_LEFT_1_PENALTY=0.080808
PRIMER_RIGHT_1_PENALTY=0.031122
PRIMER_LEFT_1_SEQUENCE=GGTGTGAGCAGTGATGTTTGTT
PRIMER_RIGHT_1_SEQUENCE=CCTCCAGTTTCTGGTAGTCACC
PRIMER_LEFT_1=34,22
PRIMER_RIGHT_1=206,22
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.1567
PRIMER_LEFT_2_PENALTY=0.125540
PRIMER_RIGHT_2_PENALTY=0.031122
PRIMER_LEFT_2_SEQUENCE=CTCTCACTCTCCTCCTCTTCCA
PRIMER_RIGHT_2_SEQUENCE=CCTCCAGTTTCTGGTAGTCACC
PRIMER_LEFT_2=73,22
PRIMER_RIGHT_2=206,22
PRIMER_LEFT_2_TM=60.126
PRIMER_RIGHT_2_TM=60.031
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=1.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=0.1928
PRIMER_LEFT_3_PENALTY=0.074695
PRIMER_RIGHT_3_PENALTY=0.118120
PRIMER_LEFT_3_SEQUENCE=ACTCTCCTCCTCTTCCATTTCC
PRIMER_RIGHT_3_SEQUENCE=TCGACAGCATCATCAACTCTTT
PRIMER_LEFT_3=78,22
PRIMER_RIGHT_3=372,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.882
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=0.1928
PRIMER_LEFT_4_PENALTY=0.074695
PRIMER_RIGHT_4_PENALTY=0.118120
PRIMER_LEFT_4_SEQUENCE=ACTCTCCTCCTCTTCCATTTCC
PRIMER_RIGHT_4_SEQUENCE=TTCGACAGCATCATCAACTCTT
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=373,22
PRIMER_LEFT_4_TM=60.075
PRIMER_RIGHT_4_TM=59.882
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_05023 Ps_contig_mira-and-tgicl-ass_franssen_36097
SEQUENCE=TCAACAATTACCGTCATGGATCGACGAATTCCTCGACTTCTCCTCGGTAAGACGAGGAGCCCACCGGCGTTCTGCCAGCGACTCTATAGCCTTCCTCGAGACATCATTTCTCGAGGAAGACGGAAAAAGTGGTGGTGATGGAGAGGGTGACTTTGATAGACTGGATGATGAACAATTAATTTCCATGTTCTCTGACAATGTTGGCGGCGGTGTCTCGTTCCCGCCTCCATCAACCTCCAACCCGTCGTCACCGTCCTCCGACCAAAATAGCAACAACAATGAACAGAAACCAATGGCAATGGCGTTGGATTCTGCACCAGTTCAAGAACGTTTTCTTCATCATGAGGTGAAGCCTAAGGATGAAGCGGTTGAAGATGAAAGCTCGTGTAGAATTGAAGAAGCGGCGGCGGCGGCGCCATCTTTCGCCGCAACCATCACCTGCACTGAAACCGTTGTTGATCCCAAGAGGGTCAAAAGAATTTTGGCAAACCGGCAATCAGCTCAGAGGTCAAGAGTGAGAAAGCTGCAATACATTTCTGAGCTTGAAAGGA
TARGET=401,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2781
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.182645
PRIMER_LEFT_SEQUENCE=TAAGGATGAAGCGGTTGAAGAT
PRIMER_RIGHT_SEQUENCE=ATTCTTTTGACCCTCTTGGGAT
PRIMER_LEFT=354,22
PRIMER_RIGHT=479,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=60.183
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=126
PRIMER_PAIR_PENALTY_1=0.2782
PRIMER_LEFT_1_PENALTY=0.095574
PRIMER_RIGHT_1_PENALTY=0.182645
PRIMER_LEFT_1_SEQUENCE=ATGAGGTGAAGCCTAAGGATGA
PRIMER_RIGHT_1_SEQUENCE=ATTCTTTTGACCCTCTTGGGAT
PRIMER_LEFT_1=341,22
PRIMER_RIGHT_1=479,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.183
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=139
PRIMER_PAIR_PENALTY_2=0.2912
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.195770
PRIMER_LEFT_2_SEQUENCE=TAAGGATGAAGCGGTTGAAGAT
PRIMER_RIGHT_2_SEQUENCE=GCAGCTTTCTCACTCTTGACCT
PRIMER_LEFT_2=354,22
PRIMER_RIGHT_2=526,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.196
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.2926
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.197140
PRIMER_LEFT_3_SEQUENCE=TAAGGATGAAGCGGTTGAAGAT
PRIMER_RIGHT_3_SEQUENCE=TTGCAGCTTTCTCACTCTTGAC
PRIMER_LEFT_3=354,22
PRIMER_RIGHT_3=528,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.2927
PRIMER_LEFT_4_PENALTY=0.095574
PRIMER_RIGHT_4_PENALTY=0.197140
PRIMER_LEFT_4_SEQUENCE=ATGAGGTGAAGCCTAAGGATGA
PRIMER_RIGHT_4_SEQUENCE=TTGCAGCTTTCTCACTCTTGAC
PRIMER_LEFT_4=341,22
PRIMER_RIGHT_4=528,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=59.803
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_05024 Ps_contig_mira-and-tgicl-ass_franssen_36117
SEQUENCE=ATGGGCGNTAAAAGGAACAGTTCGTCGAAGTACTGATGGGCATATTCATGCCAACATTGACACGGATGTAATTATTGCCGAAGAACCTCCTTATGGTTCAACACAAAAGCCTGAAGATATGTATAGGATTGTTGAACATTTTGCCCTTGGAAGGAGAAGGCTTGAACTATTTGGCGAAGACCATAATATCCGGTCCGGCTGGCTGACTCTTGGTAAAGAACTGTCGTCTTCAAATTTTAGTAAGGAGGCATATGTCAAGAACTTTGGTGACAAAGATGGGAAGGTTTGGCAGGGTGGTGGAGGAAGAAATCCACCTCCGGAGGCACCTCATCTGGTGGTGACTACTCCTGATATAGAGGCTCTTAGGCCAAAGTCCCCAATGAAGAACCAGCAACAAATGCAGCAGCAGCAGCAATGTCCATTTCTTTGACGG
TARGET=400,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05025 Ps_contig_mira-and-tgicl-ass_franssen_36123
SEQUENCE=AGAAGCCTGAATAGCAGCATCATCAAATTCTGCAGCAGAATGAATAATCGGCTGAGTTTGAGGAGGCGTGAAAAATGGCACACTATTTTCTTCTCTTGGTGGAAATTCAACTCCAGCAGCCTTTAGTTCGTTATATGCTGCATAGTATTGGGGAAAAGCTCCACTTGGGCCTCCAAAAGCTTCTTGCCATGCATCAATTAAAATCAGTATCTTTTCTCTCACACTTAAATCCGGCTTCTTCTTCACTATCTTAACCATTTCGTGCAATATATCACGCTCAATGATCTGCTGAAATACACTTTCGCCACAATTTTTAGTAAGGGTCTCCAGTACAAAAAGAGCCAGCAGCTGTGTTTTGGGATTTTTACTGCCCAAGCGCTTCTTAAGTATCTTCAGTGCATCCTTTGCTTGCCTAGGGTCCATGTTAATGATATCACACAATTCTATATTGATAGCCCAATCTGGACCAATGAGCAAGTCACTGGTTGCTCTCTCAGCACACGCAGCTGCGTTATTAGCCATCACTCTAAGCCTCCGATGAGTCAAACCCTTAACTATCAACCTTCTGTGAAAGTAGAAGAAGAAGATGAAGAATTGAGAAATGGAATTGAGAAGCGATTTTTCGATCGAGAGAGAAAGAAAGTGAGATGAGAAGATGAAGAATAA
TARGET=576,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8146
PRIMER_LEFT_PENALTY=0.122569
PRIMER_RIGHT_PENALTY=0.692052
PRIMER_LEFT_SEQUENCE=TTAGCCATCACTCTAAGCCTCC
PRIMER_RIGHT_SEQUENCE=TCGCTTCTCAATTCCATTTCTC
PRIMER_LEFT=514,22
PRIMER_RIGHT=617,22
PRIMER_LEFT_TM=59.877
PRIMER_RIGHT_TM=60.692
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=0.8628
PRIMER_LEFT_1_PENALTY=0.170703
PRIMER_RIGHT_1_PENALTY=0.692052
PRIMER_LEFT_1_SEQUENCE=TATTGATAGCCCAATCTGGACC
PRIMER_RIGHT_1_SEQUENCE=TCGCTTCTCAATTCCATTTCTC
PRIMER_LEFT_1=446,22
PRIMER_RIGHT_1=617,22
PRIMER_LEFT_1_TM=60.171
PRIMER_RIGHT_1_TM=60.692
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.9341
PRIMER_LEFT_2_PENALTY=0.122569
PRIMER_RIGHT_2_PENALTY=0.811488
PRIMER_LEFT_2_SEQUENCE=TTAGCCATCACTCTAAGCCTCC
PRIMER_RIGHT_2_SEQUENCE=TTCTCTCTCGATCGAAAAATCG
PRIMER_LEFT_2=514,22
PRIMER_RIGHT_2=636,22
PRIMER_LEFT_2_TM=59.877
PRIMER_RIGHT_2_TM=60.811
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.9344
PRIMER_LEFT_3_PENALTY=0.242357
PRIMER_RIGHT_3_PENALTY=0.692052
PRIMER_LEFT_3_SEQUENCE=GTGTTTTGGGATTTTTACTGCC
PRIMER_RIGHT_3_SEQUENCE=TCGCTTCTCAATTCCATTTCTC
PRIMER_LEFT_3=350,22
PRIMER_RIGHT_3=617,22
PRIMER_LEFT_3_TM=59.758
PRIMER_RIGHT_3_TM=60.692
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.9413
PRIMER_LEFT_4_PENALTY=0.129818
PRIMER_RIGHT_4_PENALTY=0.811488
PRIMER_LEFT_4_SEQUENCE=ATCACTCTAAGCCTCCGATGAG
PRIMER_RIGHT_4_SEQUENCE=TTCTCTCTCGATCGAAAAATCG
PRIMER_LEFT_4=520,22
PRIMER_RIGHT_4=636,22
PRIMER_LEFT_4_TM=59.870
PRIMER_RIGHT_4_TM=60.811
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=117
=
PRIMER_SEQUENCE_ID=SSR_05026 Ps_contig_mira-and-tgicl-ass_franssen_36127
SEQUENCE=CAAAACTAAATTAACTAATACCAACTCTTAATTACTCTATAATATAATCTAAACATATCAATTATTATTATTATTCCCTCATATTTTTTTTAATCTCTCATAANAAAAAATCAAGAAGAAGAAGAAGATCCATTAGTAGCACAAGCAGCAACATTAATCCCATCATTATCATTCTCATGAGCACCAAAACCACCATCGGAGACACCGTCTACGGCGGATCTTACGGCGTCGCCGTCACCATTACCATGATCTGATAATTTTCTTCCCAAAGTGTTCTTATTGTTATGCATCCAGACTTTGAGAACACTTCTATCAACACCAACTTCATTGCAAAATCCATTAACCATTTCTTCATCTCTCTTCTGCATCTTCCACCCAAGTCTCTCAGCAAATTCATGCATCTTATCCTTCTGCTCTTGTGAGAATTTTGTTCTGAATCTTTTTCTACTAGCTCCCATCGGAGAACCAAGGTTTAACGGCGCTGCTGTGTTTTCCGGCGGGATCGAGAGTCCGACTCCGGCACCAGAGAGTGCTAAGAGCATATGTGGAGCTGATGGGTATGATGAGATCGGCGGTGGAGACGGGGAGCTTGGCGAACGGGACTGAAACAACGGCGGAGGTGGTGGGTGGTGGCGGTGGTGAGGTTGATATTCAAAAACGGTGGAAATCGGTTCTTCTGGTTCACGACGGTGGAAATTCCGGTGACATCCGCAAGCGGCGCATTTTATGGAAGCGGGGTCGTCGGAGGTGGCGGTTGGTGACGGCATGAATTCACAACAACCGTCTAGTGCGTGACCACCTAAAGTTGCCACGTGGTTTTTGAGACATTCTTTGTAAGTAACAGCAACAACGTTGTTGTTGTGGTGGTTGTGAGTGTTGGGATGGTGGCGTTTAAGAACCCCATTTGAAAAAGACAGAGGCTTTGTTATGTTTGAGGTTGTTGTGTTAACGGGTTGTTGTTGTTGGATTCGGGTCGGGTTTTCTGAATCGGGTTCTGATGGTTTTGAAATTGTTGCCAAAGGAGTAGTGTTTGTGGCGGTTGTTGTTGTTGTTAAAACCTCCATCATGATAAATCTAAAAAAGATTAAAATTAAAAAAACTTACCTTAGTTTCAATTATGTGGTTCTAACTTCTAAGTTTATAAAACCTCATAAATGTATCACATCATACACTATAGTCTCCATGAAGAAGCTAGCTTGGTTTTGAGCTNTNTTTTTCNGAAAATTTGAGGAATTGAAAGGAGACAAAAAGAAAGAGGAGAAGTGGTGGTGGAGGAGGAAAAGTGGTGGTGGTTGTGGTGGTGGTGGAGGAGGAGAGGGAGGAGGAAGAGGGAGAGAATATTGAGAAAGTGGAGGTGATGTATGTGACGACTGTTGATAATCCCCGA
TARGET=61,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05027 Ps_contig_mira-and-tgicl-ass_franssen_36127
SEQUENCE=CAAAACTAAATTAACTAATACCAACTCTTAATTACTCTATAATATAATCTAAACATATCAATTATTATTATTATTCCCTCATATTTTTTTTAATCTCTCATAANAAAAAATCAAGAAGAAGAAGAAGATCCATTAGTAGCACAAGCAGCAACATTAATCCCATCATTATCATTCTCATGAGCACCAAAACCACCATCGGAGACACCGTCTACGGCGGATCTTACGGCGTCGCCGTCACCATTACCATGATCTGATAATTTTCTTCCCAAAGTGTTCTTATTGTTATGCATCCAGACTTTGAGAACACTTCTATCAACACCAACTTCATTGCAAAATCCATTAACCATTTCTTCATCTCTCTTCTGCATCTTCCACCCAAGTCTCTCAGCAAATTCATGCATCTTATCCTTCTGCTCTTGTGAGAATTTTGTTCTGAATCTTTTTCTACTAGCTCCCATCGGAGAACCAAGGTTTAACGGCGCTGCTGTGTTTTCCGGCGGGATCGAGAGTCCGACTCCGGCACCAGAGAGTGCTAAGAGCATATGTGGAGCTGATGGGTATGATGAGATCGGCGGTGGAGACGGGGAGCTTGGCGAACGGGACTGAAACAACGGCGGAGGTGGTGGGTGGTGGCGGTGGTGAGGTTGATATTCAAAAACGGTGGAAATCGGTTCTTCTGGTTCACGACGGTGGAAATTCCGGTGACATCCGCAAGCGGCGCATTTTATGGAAGCGGGGTCGTCGGAGGTGGCGGTTGGTGACGGCATGAATTCACAACAACCGTCTAGTGCGTGACCACCTAAAGTTGCCACGTGGTTTTTGAGACATTCTTTGTAAGTAACAGCAACAACGTTGTTGTTGTGGTGGTTGTGAGTGTTGGGATGGTGGCGTTTAAGAACCCCATTTGAAAAAGACAGAGGCTTTGTTATGTTTGAGGTTGTTGTGTTAACGGGTTGTTGTTGTTGGATTCGGGTCGGGTTTTCTGAATCGGGTTCTGATGGTTTTGAAATTGTTGCCAAAGGAGTAGTGTTTGTGGCGGTTGTTGTTGTTGTTAAAACCTCCATCATGATAAATCTAAAAAAGATTAAAATTAAAAAAACTTACCTTAGTTTCAATTATGTGGTTCTAACTTCTAAGTTTATAAAACCTCATAAATGTATCACATCATACACTATAGTCTCCATGAAGAAGCTAGCTTGGTTTTGAGCTNTNTTTTTCNGAAAATTTGAGGAATTGAAAGGAGACAAAAAGAAAGAGGAGAAGTGGTGGTGGAGGAGGAAAAGTGGTGGTGGTTGTGGTGGTGGTGGAGGAGGAGAGGGAGGAGGAAGAGGGAGAGAATATTGAGAAAGTGGAGGTGATGTATGTGACGACTGTTGATAATCCCCGA
TARGET=113,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05028 Ps_contig_mira-and-tgicl-ass_franssen_36127
SEQUENCE=CAAAACTAAATTAACTAATACCAACTCTTAATTACTCTATAATATAATCTAAACATATCAATTATTATTATTATTCCCTCATATTTTTTTTAATCTCTCATAANAAAAAATCAAGAAGAAGAAGAAGATCCATTAGTAGCACAAGCAGCAACATTAATCCCATCATTATCATTCTCATGAGCACCAAAACCACCATCGGAGACACCGTCTACGGCGGATCTTACGGCGTCGCCGTCACCATTACCATGATCTGATAATTTTCTTCCCAAAGTGTTCTTATTGTTATGCATCCAGACTTTGAGAACACTTCTATCAACACCAACTTCATTGCAAAATCCATTAACCATTTCTTCATCTCTCTTCTGCATCTTCCACCCAAGTCTCTCAGCAAATTCATGCATCTTATCCTTCTGCTCTTGTGAGAATTTTGTTCTGAATCTTTTTCTACTAGCTCCCATCGGAGAACCAAGGTTTAACGGCGCTGCTGTGTTTTCCGGCGGGATCGAGAGTCCGACTCCGGCACCAGAGAGTGCTAAGAGCATATGTGGAGCTGATGGGTATGATGAGATCGGCGGTGGAGACGGGGAGCTTGGCGAACGGGACTGAAACAACGGCGGAGGTGGTGGGTGGTGGCGGTGGTGAGGTTGATATTCAAAAACGGTGGAAATCGGTTCTTCTGGTTCACGACGGTGGAAATTCCGGTGACATCCGCAAGCGGCGCATTTTATGGAAGCGGGGTCGTCGGAGGTGGCGGTTGGTGACGGCATGAATTCACAACAACCGTCTAGTGCGTGACCACCTAAAGTTGCCACGTGGTTTTTGAGACATTCTTTGTAAGTAACAGCAACAACGTTGTTGTTGTGGTGGTTGTGAGTGTTGGGATGGTGGCGTTTAAGAACCCCATTTGAAAAAGACAGAGGCTTTGTTATGTTTGAGGTTGTTGTGTTAACGGGTTGTTGTTGTTGGATTCGGGTCGGGTTTTCTGAATCGGGTTCTGATGGTTTTGAAATTGTTGCCAAAGGAGTAGTGTTTGTGGCGGTTGTTGTTGTTGTTAAAACCTCCATCATGATAAATCTAAAAAAGATTAAAATTAAAAAAACTTACCTTAGTTTCAATTATGTGGTTCTAACTTCTAAGTTTATAAAACCTCATAAATGTATCACATCATACACTATAGTCTCCATGAAGAAGCTAGCTTGGTTTTGAGCTNTNTTTTTCNGAAAATTTGAGGAATTGAAAGGAGACAAAAAGAAAGAGGAGAAGTGGTGGTGGAGGAGGAAAAGTGGTGGTGGTTGTGGTGGTGGTGGAGGAGGAGAGGGAGGAGGAAGAGGGAGAGAATATTGAGAAAGTGGAGGTGATGTATGTGACGACTGTTGATAATCCCCGA
TARGET=953,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2686
PRIMER_LEFT_PENALTY=0.065292
PRIMER_RIGHT_PENALTY=0.203343
PRIMER_LEFT_SEQUENCE=TTTGAGGTTGTTGTGTTAACGG
PRIMER_RIGHT_SEQUENCE=CACAAACACTACTCCTTTGGCA
PRIMER_LEFT=930,22
PRIMER_RIGHT=1034,22
PRIMER_LEFT_TM=59.935
PRIMER_RIGHT_TM=60.203
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=105
PRIMER_PAIR_PENALTY_1=0.3055
PRIMER_LEFT_1_PENALTY=0.102129
PRIMER_RIGHT_1_PENALTY=0.203343
PRIMER_LEFT_1_SEQUENCE=CATGAATTCACAACAACCGTCT
PRIMER_RIGHT_1_SEQUENCE=CACAAACACTACTCCTTTGGCA
PRIMER_LEFT_1=764,22
PRIMER_RIGHT_1=1034,22
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=60.203
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=0.3529
PRIMER_LEFT_2_PENALTY=0.149516
PRIMER_RIGHT_2_PENALTY=0.203343
PRIMER_LEFT_2_SEQUENCE=GAACCCCATTTGAAAAAGACAG
PRIMER_RIGHT_2_SEQUENCE=CACAAACACTACTCCTTTGGCA
PRIMER_LEFT_2=895,22
PRIMER_RIGHT_2=1034,22
PRIMER_LEFT_2_TM=59.850
PRIMER_RIGHT_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=0.4410
PRIMER_LEFT_3_PENALTY=0.237699
PRIMER_RIGHT_3_PENALTY=0.203343
PRIMER_LEFT_3_SEQUENCE=CCATTTGAAAAAGACAGAGGCT
PRIMER_RIGHT_3_SEQUENCE=CACAAACACTACTCCTTTGGCA
PRIMER_LEFT_3=900,22
PRIMER_RIGHT_3=1034,22
PRIMER_LEFT_3_TM=59.762
PRIMER_RIGHT_3_TM=60.203
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=0.6285
PRIMER_LEFT_4_PENALTY=0.425131
PRIMER_RIGHT_4_PENALTY=0.203343
PRIMER_LEFT_4_SEQUENCE=CTAAAGTTGCCACGTGGTTTTT
PRIMER_RIGHT_4_SEQUENCE=CACAAACACTACTCCTTTGGCA
PRIMER_LEFT_4=799,22
PRIMER_RIGHT_4=1034,22
PRIMER_LEFT_4_TM=60.425
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_05029 Ps_contig_mira-and-tgicl-ass_franssen_36127
SEQUENCE=CAAAACTAAATTAACTAATACCAACTCTTAATTACTCTATAATATAATCTAAACATATCAATTATTATTATTATTCCCTCATATTTTTTTTAATCTCTCATAANAAAAAATCAAGAAGAAGAAGAAGATCCATTAGTAGCACAAGCAGCAACATTAATCCCATCATTATCATTCTCATGAGCACCAAAACCACCATCGGAGACACCGTCTACGGCGGATCTTACGGCGTCGCCGTCACCATTACCATGATCTGATAATTTTCTTCCCAAAGTGTTCTTATTGTTATGCATCCAGACTTTGAGAACACTTCTATCAACACCAACTTCATTGCAAAATCCATTAACCATTTCTTCATCTCTCTTCTGCATCTTCCACCCAAGTCTCTCAGCAAATTCATGCATCTTATCCTTCTGCTCTTGTGAGAATTTTGTTCTGAATCTTTTTCTACTAGCTCCCATCGGAGAACCAAGGTTTAACGGCGCTGCTGTGTTTTCCGGCGGGATCGAGAGTCCGACTCCGGCACCAGAGAGTGCTAAGAGCATATGTGGAGCTGATGGGTATGATGAGATCGGCGGTGGAGACGGGGAGCTTGGCGAACGGGACTGAAACAACGGCGGAGGTGGTGGGTGGTGGCGGTGGTGAGGTTGATATTCAAAAACGGTGGAAATCGGTTCTTCTGGTTCACGACGGTGGAAATTCCGGTGACATCCGCAAGCGGCGCATTTTATGGAAGCGGGGTCGTCGGAGGTGGCGGTTGGTGACGGCATGAATTCACAACAACCGTCTAGTGCGTGACCACCTAAAGTTGCCACGTGGTTTTTGAGACATTCTTTGTAAGTAACAGCAACAACGTTGTTGTTGTGGTGGTTGTGAGTGTTGGGATGGTGGCGTTTAAGAACCCCATTTGAAAAAGACAGAGGCTTTGTTATGTTTGAGGTTGTTGTGTTAACGGGTTGTTGTTGTTGGATTCGGGTCGGGTTTTCTGAATCGGGTTCTGATGGTTTTGAAATTGTTGCCAAAGGAGTAGTGTTTGTGGCGGTTGTTGTTGTTGTTAAAACCTCCATCATGATAAATCTAAAAAAGATTAAAATTAAAAAAACTTACCTTAGTTTCAATTATGTGGTTCTAACTTCTAAGTTTATAAAACCTCATAAATGTATCACATCATACACTATAGTCTCCATGAAGAAGCTAGCTTGGTTTTGAGCTNTNTTTTTCNGAAAATTTGAGGAATTGAAAGGAGACAAAAAGAAAGAGGAGAAGTGGTGGTGGAGGAGGAAAAGTGGTGGTGGTTGTGGTGGTGGTGGAGGAGGAGAGGGAGGAGGAAGAGGGAGAGAATATTGAGAAAGTGGAGGTGATGTATGTGACGACTGTTGATAATCCCCGA
TARGET=1039,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2805
PRIMER_LEFT_PENALTY=1.058858
PRIMER_RIGHT_PENALTY=0.221594
PRIMER_LEFT_SEQUENCE=TTTCTGAATCGGGTTCTGATG
PRIMER_RIGHT_SEQUENCE=CACCACCACTTCTCCTCTTTCT
PRIMER_LEFT=979,21
PRIMER_RIGHT=1270,22
PRIMER_LEFT_TM=60.059
PRIMER_RIGHT_TM=59.778
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=1.4018
PRIMER_LEFT_1_PENALTY=1.180191
PRIMER_RIGHT_1_PENALTY=0.221594
PRIMER_LEFT_1_SEQUENCE=GAATCGGGTTCTGATGGTTTT
PRIMER_RIGHT_1_SEQUENCE=CACCACCACTTCTCCTCTTTCT
PRIMER_LEFT_1=984,21
PRIMER_RIGHT_1=1270,22
PRIMER_LEFT_1_TM=60.180
PRIMER_RIGHT_1_TM=59.778
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=1.5491
PRIMER_LEFT_2_PENALTY=1.327484
PRIMER_RIGHT_2_PENALTY=0.221594
PRIMER_LEFT_2_SEQUENCE=TTTTCTGAATCGGGTTCTGATG
PRIMER_RIGHT_2_SEQUENCE=CACCACCACTTCTCCTCTTTCT
PRIMER_LEFT_2=978,22
PRIMER_RIGHT_2=1270,22
PRIMER_LEFT_2_TM=61.327
PRIMER_RIGHT_2_TM=59.778
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=2.0357
PRIMER_LEFT_3_PENALTY=0.931499
PRIMER_RIGHT_3_PENALTY=1.104205
PRIMER_LEFT_3_SEQUENCE=CCAAAGGAGTAGTGTTTGTGGC
PRIMER_RIGHT_3_SEQUENCE=CCACCACTTCTCCTCTTTCTTT
PRIMER_LEFT_3=1015,22
PRIMER_RIGHT_3=1268,22
PRIMER_LEFT_3_TM=60.931
PRIMER_RIGHT_3_TM=58.896
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=2.0796
PRIMER_LEFT_4_PENALTY=0.931499
PRIMER_RIGHT_4_PENALTY=1.148090
PRIMER_LEFT_4_SEQUENCE=CCAAAGGAGTAGTGTTTGTGGC
PRIMER_RIGHT_4_SEQUENCE=CCACCACTTCTCCTCTTTCTTTT
PRIMER_LEFT_4=1015,22
PRIMER_RIGHT_4=1268,23
PRIMER_LEFT_4_TM=60.931
PRIMER_RIGHT_4_TM=60.148
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_05030 Ps_contig_mira-and-tgicl-ass_franssen_36127
SEQUENCE=CAAAACTAAATTAACTAATACCAACTCTTAATTACTCTATAATATAATCTAAACATATCAATTATTATTATTATTCCCTCATATTTTTTTTAATCTCTCATAANAAAAAATCAAGAAGAAGAAGAAGATCCATTAGTAGCACAAGCAGCAACATTAATCCCATCATTATCATTCTCATGAGCACCAAAACCACCATCGGAGACACCGTCTACGGCGGATCTTACGGCGTCGCCGTCACCATTACCATGATCTGATAATTTTCTTCCCAAAGTGTTCTTATTGTTATGCATCCAGACTTTGAGAACACTTCTATCAACACCAACTTCATTGCAAAATCCATTAACCATTTCTTCATCTCTCTTCTGCATCTTCCACCCAAGTCTCTCAGCAAATTCATGCATCTTATCCTTCTGCTCTTGTGAGAATTTTGTTCTGAATCTTTTTCTACTAGCTCCCATCGGAGAACCAAGGTTTAACGGCGCTGCTGTGTTTTCCGGCGGGATCGAGAGTCCGACTCCGGCACCAGAGAGTGCTAAGAGCATATGTGGAGCTGATGGGTATGATGAGATCGGCGGTGGAGACGGGGAGCTTGGCGAACGGGACTGAAACAACGGCGGAGGTGGTGGGTGGTGGCGGTGGTGAGGTTGATATTCAAAAACGGTGGAAATCGGTTCTTCTGGTTCACGACGGTGGAAATTCCGGTGACATCCGCAAGCGGCGCATTTTATGGAAGCGGGGTCGTCGGAGGTGGCGGTTGGTGACGGCATGAATTCACAACAACCGTCTAGTGCGTGACCACCTAAAGTTGCCACGTGGTTTTTGAGACATTCTTTGTAAGTAACAGCAACAACGTTGTTGTTGTGGTGGTTGTGAGTGTTGGGATGGTGGCGTTTAAGAACCCCATTTGAAAAAGACAGAGGCTTTGTTATGTTTGAGGTTGTTGTGTTAACGGGTTGTTGTTGTTGGATTCGGGTCGGGTTTTCTGAATCGGGTTCTGATGGTTTTGAAATTGTTGCCAAAGGAGTAGTGTTTGTGGCGGTTGTTGTTGTTGTTAAAACCTCCATCATGATAAATCTAAAAAAGATTAAAATTAAAAAAACTTACCTTAGTTTCAATTATGTGGTTCTAACTTCTAAGTTTATAAAACCTCATAAATGTATCACATCATACACTATAGTCTCCATGAAGAAGCTAGCTTGGTTTTGAGCTNTNTTTTTCNGAAAATTTGAGGAATTGAAAGGAGACAAAAAGAAAGAGGAGAAGTGGTGGTGGAGGAGGAAAAGTGGTGGTGGTTGTGGTGGTGGTGGAGGAGGAGAGGGAGGAGGAAGAGGGAGAGAATATTGAGAAAGTGGAGGTGATGTATGTGACGACTGTTGATAATCCCCGA
TARGET=1295,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3624
PRIMER_LEFT_PENALTY=0.221594
PRIMER_RIGHT_PENALTY=0.140832
PRIMER_LEFT_SEQUENCE=AGAAAGAGGAGAAGTGGTGGTG
PRIMER_RIGHT_SEQUENCE=GGGATTATCAACAGTCGTCACA
PRIMER_LEFT=1249,22
PRIMER_RIGHT=1383,22
PRIMER_LEFT_TM=59.778
PRIMER_RIGHT_TM=59.859
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=0.8406
PRIMER_LEFT_1_PENALTY=0.221594
PRIMER_RIGHT_1_PENALTY=0.619003
PRIMER_LEFT_1_SEQUENCE=AGAAAGAGGAGAAGTGGTGGTG
PRIMER_RIGHT_1_SEQUENCE=GGGGATTATCAACAGTCGTCAC
PRIMER_LEFT_1=1249,22
PRIMER_RIGHT_1=1384,22
PRIMER_LEFT_1_TM=59.778
PRIMER_RIGHT_1_TM=60.619
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=1.2450
PRIMER_LEFT_2_PENALTY=1.104205
PRIMER_RIGHT_2_PENALTY=0.140832
PRIMER_LEFT_2_SEQUENCE=AAAGAAAGAGGAGAAGTGGTGG
PRIMER_RIGHT_2_SEQUENCE=GGGATTATCAACAGTCGTCACA
PRIMER_LEFT_2=1247,22
PRIMER_RIGHT_2=1383,22
PRIMER_LEFT_2_TM=58.896
PRIMER_RIGHT_2_TM=59.859
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=1.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=1.2889
PRIMER_LEFT_3_PENALTY=1.148090
PRIMER_RIGHT_3_PENALTY=0.140832
PRIMER_LEFT_3_SEQUENCE=AAAAGAAAGAGGAGAAGTGGTGG
PRIMER_RIGHT_3_SEQUENCE=GGGATTATCAACAGTCGTCACA
PRIMER_LEFT_3=1246,23
PRIMER_RIGHT_3=1383,22
PRIMER_LEFT_3_TM=60.148
PRIMER_RIGHT_3_TM=59.859
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=1.3522
PRIMER_LEFT_4_PENALTY=0.221594
PRIMER_RIGHT_4_PENALTY=1.130619
PRIMER_LEFT_4_SEQUENCE=AGAAAGAGGAGAAGTGGTGGTG
PRIMER_RIGHT_4_SEQUENCE=GGGATTATCAACAGTCGTCACAT
PRIMER_LEFT_4=1249,22
PRIMER_RIGHT_4=1383,23
PRIMER_LEFT_4_TM=59.778
PRIMER_RIGHT_4_TM=60.131
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=135
=
PRIMER_SEQUENCE_ID=SSR_05031 Ps_contig_mira-and-tgicl-ass_franssen_36148
SEQUENCE=TTTGTCGCTAGCCGGATCGTTTCTTCCGCCGCCGGCTCCGCCTGCCGCTTCTGGTTTGACTATATATCTGGCAGGAGGACAAGGACAGGTTGTTGGTGGAAGTGTTGTTGGTGCTTTGATTGCTTCTGGACCCGTTGTCATCATGTCTGCTTCTTTTAGTAACGCTGCTTATGAGAGACTTCCCTTGGAAGAAGATGATGGTTCTTCTTTGCAACAACTTCAAGGTGGTGGTGGTGGTGGTGGTTCTCCAAGTGGTGGTGGTGGTGGCGGTGG
TARGET=224,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05032 Ps_contig_mira-and-tgicl-ass_franssen_36148
SEQUENCE=TTTGTCGCTAGCCGGATCGTTTCTTCCGCCGCCGGCTCCGCCTGCCGCTTCTGGTTTGACTATATATCTGGCAGGAGGACAAGGACAGGTTGTTGGTGGAAGTGTTGTTGGTGCTTTGATTGCTTCTGGACCCGTTGTCATCATGTCTGCTTCTTTTAGTAACGCTGCTTATGAGAGACTTCCCTTGGAAGAAGATGATGGTTCTTCTTTGCAACAACTTCAAGGTGGTGGTGGTGGTGGTGGTTCTCCAAGTGGTGGTGGTGGTGGCGGTGG
TARGET=252,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05033 Ps_contig_mira-and-tgicl-ass_franssen_36173
SEQUENCE=GTTTAAAATCCTNANNGATTGAAAGATATTTATATATTATGTTAATAAAAGGGAATCAATGAGAGTTTTGGATATAGATTCAATCATAAAATAAAAGAGCTCAGATCCTCTCTATTTCTCAAAAGAAATGAAAGCCGGACGGCAACCGATACAACTCGAGCATATAAAACTACTAGTTTTCATTAACTAGGCGCCTTTCCGTTTCCGAGAGTAAGCTCCAAATCATCCAATCCCACTTCATGAATCCTTTCTCCAACCCAAGGCTTTACCACCTGCGCTCCGAACTCCGGCTCTGAAATCCTCATCTCTTGCATTCTGTTTTCAGGTAGCATGTTATGCTGCTCATTAATTACAGGTTTCAGAAAGTTCAATGTAGGAGAGACTGATGATTTAGCAGAAGCTGCAAAGGCTTGGAAGTTCAACCACTGATTAGACTCGACAGTAGAAGTATCAGACTCGTCGCATTCAGGTATAGTAGGTGGTGTATAGAGGTGACGGTGCGACGGGCTAGACGGTGCGGAAGCTGCAAAGAAAGGGTGATTGAAGGATGTAATGGATGCTTTGGCGATTGAGTCCCATGTGGGGATCATGGGTTTGGGATTTCTCGAGGTTGGTGATGAGATAGGTGGCGTCACAGGTGCACTGTTTGATATTCTTAAAGGCGGGAGAGACATATTGGGGAACACGCTGCGAACGTATGGAATGAAATTTGATGGGTTATTATTTGTATCCATACGAGACGGGCTCGGAAATGAAGAGGAGGAGGGGCTAACTTGGTAGGAATGGATTGGACTTGGAAATGACGACGAAAGAGGACTCGGATTTTGCGATGAAAATAGGTTGTTTCTGGTACAAGTTCCAGCACCATCGCCTGATGTTGCCGCCCTTGATCCTCTTCGATAGGTAGTGCCGTCTTCTTCAACGGTCCAACCAGCTTCAACACAGAGAGCTTTCAAGACCTCATTGTTATCACAGTGTTTAGGCAAATTATAATTCCCCTGAGACCTCAATCCCGCGTAAATCTTCGCCGCTATAGCTCTCCTCCGTCTCTCTCTCCTCCTGTTGTTCTCTCTCTCCCTCCACGACGGTTTCCTCCGACTGTTCGCCGCCGAAGTTGCTCCGTCAGAACCCATTCACACAGACTCAGAGTGTGTAGCTGTAGAACGCGTCTTAGGTCCCCGA
TARGET=1047,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0972
PRIMER_LEFT_PENALTY=0.088367
PRIMER_RIGHT_PENALTY=0.008837
PRIMER_LEFT_SEQUENCE=CAACCAGCTTCAACACAGAGAG
PRIMER_RIGHT_SEQUENCE=GACGCGTTCTACAGCTACACAC
PRIMER_LEFT=927,22
PRIMER_RIGHT=1169,22
PRIMER_LEFT_TM=60.088
PRIMER_RIGHT_TM=60.009
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.1924
PRIMER_LEFT_1_PENALTY=0.183585
PRIMER_RIGHT_1_PENALTY=0.008837
PRIMER_LEFT_1_SEQUENCE=AGGTAGTGCCGTCTTCTTCAAC
PRIMER_RIGHT_1_SEQUENCE=GACGCGTTCTACAGCTACACAC
PRIMER_LEFT_1=901,22
PRIMER_RIGHT_1=1169,22
PRIMER_LEFT_1_TM=59.816
PRIMER_RIGHT_1_TM=60.009
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=0.2044
PRIMER_LEFT_2_PENALTY=0.195574
PRIMER_RIGHT_2_PENALTY=0.008837
PRIMER_LEFT_2_SEQUENCE=CGATAGGTAGTGCCGTCTTCTT
PRIMER_RIGHT_2_SEQUENCE=GACGCGTTCTACAGCTACACAC
PRIMER_LEFT_2=897,22
PRIMER_RIGHT_2=1169,22
PRIMER_LEFT_2_TM=59.804
PRIMER_RIGHT_2_TM=60.009
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.2044
PRIMER_LEFT_3_PENALTY=0.195574
PRIMER_RIGHT_3_PENALTY=0.008837
PRIMER_LEFT_3_SEQUENCE=CTTCGATAGGTAGTGCCGTCTT
PRIMER_RIGHT_3_SEQUENCE=GACGCGTTCTACAGCTACACAC
PRIMER_LEFT_3=894,22
PRIMER_RIGHT_3=1169,22
PRIMER_LEFT_3_TM=59.804
PRIMER_RIGHT_3_TM=60.009
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.2271
PRIMER_LEFT_4_PENALTY=0.088367
PRIMER_RIGHT_4_PENALTY=0.138756
PRIMER_LEFT_4_SEQUENCE=CAACCAGCTTCAACACAGAGAG
PRIMER_RIGHT_4_SEQUENCE=AGACGCGTTCTACAGCTACACA
PRIMER_LEFT_4=927,22
PRIMER_RIGHT_4=1170,22
PRIMER_LEFT_4_TM=60.088
PRIMER_RIGHT_4_TM=60.139
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=244
=
PRIMER_SEQUENCE_ID=SSR_05034 Ps_contig_mira-and-tgicl-ass_franssen_36173
SEQUENCE=GTTTAAAATCCTNANNGATTGAAAGATATTTATATATTATGTTAATAAAAGGGAATCAATGAGAGTTTTGGATATAGATTCAATCATAAAATAAAAGAGCTCAGATCCTCTCTATTTCTCAAAAGAAATGAAAGCCGGACGGCAACCGATACAACTCGAGCATATAAAACTACTAGTTTTCATTAACTAGGCGCCTTTCCGTTTCCGAGAGTAAGCTCCAAATCATCCAATCCCACTTCATGAATCCTTTCTCCAACCCAAGGCTTTACCACCTGCGCTCCGAACTCCGGCTCTGAAATCCTCATCTCTTGCATTCTGTTTTCAGGTAGCATGTTATGCTGCTCATTAATTACAGGTTTCAGAAAGTTCAATGTAGGAGAGACTGATGATTTAGCAGAAGCTGCAAAGGCTTGGAAGTTCAACCACTGATTAGACTCGACAGTAGAAGTATCAGACTCGTCGCATTCAGGTATAGTAGGTGGTGTATAGAGGTGACGGTGCGACGGGCTAGACGGTGCGGAAGCTGCAAAGAAAGGGTGATTGAAGGATGTAATGGATGCTTTGGCGATTGAGTCCCATGTGGGGATCATGGGTTTGGGATTTCTCGAGGTTGGTGATGAGATAGGTGGCGTCACAGGTGCACTGTTTGATATTCTTAAAGGCGGGAGAGACATATTGGGGAACACGCTGCGAACGTATGGAATGAAATTTGATGGGTTATTATTTGTATCCATACGAGACGGGCTCGGAAATGAAGAGGAGGAGGGGCTAACTTGGTAGGAATGGATTGGACTTGGAAATGACGACGAAAGAGGACTCGGATTTTGCGATGAAAATAGGTTGTTTCTGGTACAAGTTCCAGCACCATCGCCTGATGTTGCCGCCCTTGATCCTCTTCGATAGGTAGTGCCGTCTTCTTCAACGGTCCAACCAGCTTCAACACAGAGAGCTTTCAAGACCTCATTGTTATCACAGTGTTTAGGCAAATTATAATTCCCCTGAGACCTCAATCCCGCGTAAATCTTCGCCGCTATAGCTCTCCTCCGTCTCTCTCTCCTCCTGTTGTTCTCTCTCTCCCTCCACGACGGTTTCCTCCGACTGTTCGCCGCCGAAGTTGCTCCGTCAGAACCCATTCACACAGACTCAGAGTGTGTAGCTGTAGAACGCGTCTTAGGTCCCCGA
TARGET=1067,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0602
PRIMER_LEFT_PENALTY=0.051345
PRIMER_RIGHT_PENALTY=0.008837
PRIMER_LEFT_SEQUENCE=CGTCTCTCTCTCCTCCTGTTGT
PRIMER_RIGHT_SEQUENCE=GACGCGTTCTACAGCTACACAC
PRIMER_LEFT=1044,22
PRIMER_RIGHT=1169,22
PRIMER_LEFT_TM=60.051
PRIMER_RIGHT_TM=60.009
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=126
PRIMER_PAIR_PENALTY_1=0.0972
PRIMER_LEFT_1_PENALTY=0.088367
PRIMER_RIGHT_1_PENALTY=0.008837
PRIMER_LEFT_1_SEQUENCE=CAACCAGCTTCAACACAGAGAG
PRIMER_RIGHT_1_SEQUENCE=GACGCGTTCTACAGCTACACAC
PRIMER_LEFT_1=927,22
PRIMER_RIGHT_1=1169,22
PRIMER_LEFT_1_TM=60.088
PRIMER_RIGHT_1_TM=60.009
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.1901
PRIMER_LEFT_2_PENALTY=0.051345
PRIMER_RIGHT_2_PENALTY=0.138756
PRIMER_LEFT_2_SEQUENCE=CGTCTCTCTCTCCTCCTGTTGT
PRIMER_RIGHT_2_SEQUENCE=CGCGTTCTACAGCTACACACTC
PRIMER_LEFT_2=1044,22
PRIMER_RIGHT_2=1167,22
PRIMER_LEFT_2_TM=60.051
PRIMER_RIGHT_2_TM=60.139
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=124
PRIMER_PAIR_PENALTY_3=0.1901
PRIMER_LEFT_3_PENALTY=0.051345
PRIMER_RIGHT_3_PENALTY=0.138756
PRIMER_LEFT_3_SEQUENCE=CGTCTCTCTCTCCTCCTGTTGT
PRIMER_RIGHT_3_SEQUENCE=AGACGCGTTCTACAGCTACACA
PRIMER_LEFT_3=1044,22
PRIMER_RIGHT_3=1170,22
PRIMER_LEFT_3_TM=60.051
PRIMER_RIGHT_3_TM=60.139
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=0.1924
PRIMER_LEFT_4_PENALTY=0.183585
PRIMER_RIGHT_4_PENALTY=0.008837
PRIMER_LEFT_4_SEQUENCE=AGGTAGTGCCGTCTTCTTCAAC
PRIMER_RIGHT_4_SEQUENCE=GACGCGTTCTACAGCTACACAC
PRIMER_LEFT_4=901,22
PRIMER_RIGHT_4=1169,22
PRIMER_LEFT_4_TM=59.816
PRIMER_RIGHT_4_TM=60.009
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_05035 Ps_contig_mira-and-tgicl-ass_franssen_36177
SEQUENCE=CCTTTTTTATTTACTAAATTACATTTCTACCATTTCATTNAAACCAAAGTCATGTTACAACCACTTCATTACATTTTAATTTCCATCTTCATACAAATACATTCCAATTTCATTACATTTCAATTCAACACTTTCATACAAGTTCATTCTAAGACATGGCCATGCATTACAAGTCCAGTTGACCATACATTCATCTACAATCCATTACATCCAATTTCAAATTAAAAAGCCATTTCTATACAATTCCAAAATACAAAACATTTATTCATACATAACCCTATATAAACCAACTACAGAGCTCAAATCCAATAGCTCAAGCACATTCCAAGTATCTCTGATTAACAACATGTCCAAGTATAAAACCAATCACCAAGTCATTGGCCAAACAGTCCAAAGCACTCCATTGAATCCAAGCCAGTCCATATGCAATGCACTTCAAGGCATCAAGGAAAGGAACTTCAAGCATACTGACGCCGGCAAAAGATCCGGTGAGCCTCCAAATATTTTTCTTCTTTTTTGAGTTTCTAGGTTACCAAAGTGTAGAGTTTCACGAGGGGAATAAAATCCCCAGCGTGTGGTGGCTTGATTCCCCTCCGTTTCCATTTAATTTAGGATTCCGGTGTTAGGGCTCTTGGAGACTTCACTTCAATTCGCTTAATTCTTGTAGCAGTAGAATATTTCACTTACAGATTCGTGACCAGGGCGTGATTTCGAGTGGTATGTTTGCTTTTAATTAGGTTCTTGAGAACCTCCGCAGTCGAGGACCGCTCCGGCACGGCGGAGGTAATAACTGAACATGAGCTGAGAGAGAGATGTGAGTGTTCTTATTCTCAGTTTTCTTTATTCCATTTTTGGCACGTGCATTTTTAATGTTNCCCCCGAC
TARGET=804,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0301
PRIMER_LEFT_PENALTY=0.003166
PRIMER_RIGHT_PENALTY=1.026932
PRIMER_LEFT_SEQUENCE=TGATTTCGAGTGGTATGTTTGC
PRIMER_RIGHT_SEQUENCE=GGGNAACATTAAAAATGCACG
PRIMER_LEFT=705,22
PRIMER_RIGHT=877,21
PRIMER_LEFT_TM=60.003
PRIMER_RIGHT_TM=59.973
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=1.1351
PRIMER_LEFT_1_PENALTY=0.108206
PRIMER_RIGHT_1_PENALTY=1.026932
PRIMER_LEFT_1_SEQUENCE=GGAGACTTCACTTCAATTCGCT
PRIMER_RIGHT_1_SEQUENCE=GGGNAACATTAAAAATGCACG
PRIMER_LEFT_1=633,22
PRIMER_RIGHT_1=877,21
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=59.973
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=1.1414
PRIMER_LEFT_2_PENALTY=0.114470
PRIMER_RIGHT_2_PENALTY=1.026932
PRIMER_LEFT_2_SEQUENCE=TTAGGGCTCTTGGAGACTTCAC
PRIMER_RIGHT_2_SEQUENCE=GGGNAACATTAAAAATGCACG
PRIMER_LEFT_2=622,22
PRIMER_RIGHT_2=877,21
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=59.973
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=1.1414
PRIMER_LEFT_3_PENALTY=0.114470
PRIMER_RIGHT_3_PENALTY=1.026932
PRIMER_LEFT_3_SEQUENCE=TGTTAGGGCTCTTGGAGACTTC
PRIMER_RIGHT_3_SEQUENCE=GGGNAACATTAAAAATGCACG
PRIMER_LEFT_3=620,22
PRIMER_RIGHT_3=877,21
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=59.973
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=1.3105
PRIMER_LEFT_4_PENALTY=0.003166
PRIMER_RIGHT_4_PENALTY=1.307346
PRIMER_LEFT_4_SEQUENCE=TGATTTCGAGTGGTATGTTTGC
PRIMER_RIGHT_4_SEQUENCE=GTCGGGGGNAACATTAAAAAT
PRIMER_LEFT_4=705,22
PRIMER_RIGHT_4=882,21
PRIMER_LEFT_4_TM=60.003
PRIMER_RIGHT_4_TM=59.693
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_05036 Ps_contig_mira-and-tgicl-ass_franssen_36189
SEQUENCE=ATAATAATAATAATAATAAAGGTCATATCATAGCAGAGAGAAAGCGAAGAGAGAAACTTAACAAGAACTTAATTGCTTTAGCTGCTCTTATTCCTGGATTAAAGAAGGTACCAAACCATTCCTTTGACCTTTCATTCCAAATGTTCGGTACTTTACAGATTTTGTGTTATGTTGTGAATCAATCATTCTACAGATGGACAAGGCTTCAGTGCTGGGAGATGCTATAAAGTACATGAAAGAGCTCCAAGAACGTTTGAAGGTATTAGAAGAACAGAATAAGAA
TARGET=1,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05037 Ps_contig_mira-and-tgicl-ass_franssen_36193
SEQUENCE=CCTTTCCTTCTTCCTACTTTTTAATTAGTTCTTTTTAGGGTAAGGAAAAAAGAAAATAGACGAGTGTGAGTGAGTGCTACCTTTCAAACTCGAAAAGATGGAGAAAATCAACTTTGTCAAAAATAGGTGAATTGAAAATGCCACCAGGTTTTCGTTTTCATCCAACCGATGAAGAACTTGTTCTTTCCTATTTGAAACGTAAGGTCTTCTCTCTCCCTTTGCCTGCTGCAATCATCCCTGAAGTTGATCTCTGCAAGTCCGACCCATGGGATTTACCCGGTGATTTGGAACAAGAAAGGTACTTTTTCAGTACCAAAGAAGCGAAATATCCGAATGGGAACCGATCTAACAGAGCCACAAATTCTGGCTACTGGAAGGCTACTGGTTTGGACAAACAGATTATTAGCTGGTCGAAATCTGAGGTTGTCGGGATGAAGAAGACTCTTGTTTTTTACGGCGGTAAACCACCTCATGGTTCCAGAACTGATTGGATTATGCATGAGTATCGTCTTGCTCCTTCTCACTCTAACCCTATGAATGAAAACTGGGTTCTGTGTCGCATATTTCTGAAGAAAAGAGCTGGTACTAAGAACGGAAGAGAAACAGACACTGTGAAACCAAACATCATGGTCAACGACAACAACCTTGGCAAGGTGGATAACTCAGGTTCAAAGGTTGTTGTTTTCTATGATTTCTTGGCACAGAGCAACAACAACAAGACTGTTGCATCTTCAATCTCACCAGCTACAAGTGGAATCACAGATGAATTGAATGAGCATGAAAATGAAGAAAGCAGTAGCTCTTAACAAACATTGAATTTAATGTTTGCTTTATTAATTATCTTTTTATTATTATTAATATTAATATTATTATTATTATCTATGTAAGTAGTAATTATGTTATGCGGCATTAAGTTTCACAGCTCGAAAGAGTACTAATGTAATATGTAATATTAATATTAAAATRGAAATGGAATTTTTTTATTTTCCTTAACGT
TARGET=707,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05038 Ps_contig_mira-and-tgicl-ass_franssen_36193
SEQUENCE=CCTTTCCTTCTTCCTACTTTTTAATTAGTTCTTTTTAGGGTAAGGAAAAAAGAAAATAGACGAGTGTGAGTGAGTGCTACCTTTCAAACTCGAAAAGATGGAGAAAATCAACTTTGTCAAAAATAGGTGAATTGAAAATGCCACCAGGTTTTCGTTTTCATCCAACCGATGAAGAACTTGTTCTTTCCTATTTGAAACGTAAGGTCTTCTCTCTCCCTTTGCCTGCTGCAATCATCCCTGAAGTTGATCTCTGCAAGTCCGACCCATGGGATTTACCCGGTGATTTGGAACAAGAAAGGTACTTTTTCAGTACCAAAGAAGCGAAATATCCGAATGGGAACCGATCTAACAGAGCCACAAATTCTGGCTACTGGAAGGCTACTGGTTTGGACAAACAGATTATTAGCTGGTCGAAATCTGAGGTTGTCGGGATGAAGAAGACTCTTGTTTTTTACGGCGGTAAACCACCTCATGGTTCCAGAACTGATTGGATTATGCATGAGTATCGTCTTGCTCCTTCTCACTCTAACCCTATGAATGAAAACTGGGTTCTGTGTCGCATATTTCTGAAGAAAAGAGCTGGTACTAAGAACGGAAGAGAAACAGACACTGTGAAACCAAACATCATGGTCAACGACAACAACCTTGGCAAGGTGGATAACTCAGGTTCAAAGGTTGTTGTTTTCTATGATTTCTTGGCACAGAGCAACAACAACAAGACTGTTGCATCTTCAATCTCACCAGCTACAAGTGGAATCACAGATGAATTGAATGAGCATGAAAATGAAGAAAGCAGTAGCTCTTAACAAACATTGAATTTAATGTTTGCTTTATTAATTATCTTTTTATTATTATTAATATTAATATTATTATTATTATCTATGTAAGTAGTAATTATGTTATGCGGCATTAAGTTTCACAGCTCGAAAGAGTACTAATGTAATATGTAATATTAATATTAAAATRGAAATGGAATTTTTTTATTTTCCTTAACGT
TARGET=846,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05039 Ps_contig_mira-and-tgicl-ass_franssen_36193
SEQUENCE=CCTTTCCTTCTTCCTACTTTTTAATTAGTTCTTTTTAGGGTAAGGAAAAAAGAAAATAGACGAGTGTGAGTGAGTGCTACCTTTCAAACTCGAAAAGATGGAGAAAATCAACTTTGTCAAAAATAGGTGAATTGAAAATGCCACCAGGTTTTCGTTTTCATCCAACCGATGAAGAACTTGTTCTTTCCTATTTGAAACGTAAGGTCTTCTCTCTCCCTTTGCCTGCTGCAATCATCCCTGAAGTTGATCTCTGCAAGTCCGACCCATGGGATTTACCCGGTGATTTGGAACAAGAAAGGTACTTTTTCAGTACCAAAGAAGCGAAATATCCGAATGGGAACCGATCTAACAGAGCCACAAATTCTGGCTACTGGAAGGCTACTGGTTTGGACAAACAGATTATTAGCTGGTCGAAATCTGAGGTTGTCGGGATGAAGAAGACTCTTGTTTTTTACGGCGGTAAACCACCTCATGGTTCCAGAACTGATTGGATTATGCATGAGTATCGTCTTGCTCCTTCTCACTCTAACCCTATGAATGAAAACTGGGTTCTGTGTCGCATATTTCTGAAGAAAAGAGCTGGTACTAAGAACGGAAGAGAAACAGACACTGTGAAACCAAACATCATGGTCAACGACAACAACCTTGGCAAGGTGGATAACTCAGGTTCAAAGGTTGTTGTTTTCTATGATTTCTTGGCACAGAGCAACAACAACAAGACTGTTGCATCTTCAATCTCACCAGCTACAAGTGGAATCACAGATGAATTGAATGAGCATGAAAATGAAGAAAGCAGTAGCTCTTAACAAACATTGAATTTAATGTTTGCTTTATTAATTATCTTTTTATTATTATTAATATTAATATTATTATTATTATCTATGTAAGTAGTAATTATGTTATGCGGCATTAAGTTTCACAGCTCGAAAGAGTACTAATGTAATATGTAATATTAATATTAAAATRGAAATGGAATTTTTTTATTTTCCTTAACGT
TARGET=865,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05040 Ps_contig_mira-and-tgicl-ass_franssen_36201
SEQUENCE=GTCGGGGAACACTCACTAGATTACTCTTCTTTCTTTCTTGTCCTTGCTCCAAAAAATGGCTTCAAAATCCATTCTTCTTCTAGGCATTTTTCTTGTGGTGGCCACTAAGGTTTATTCGTATGAGGAAGATCTCAAAATAGTGGTGAACTATGCAAACCCTTCAGCTCCTTCTGTTCTTGCTCCACCAGTAAAACCAACGCCAACACCTGTAGTGACGGCTCCAACACCACCTTCTCCGGTGAAGACACCGCCAACAACTCCACCATATGTCAAGGCACCTCCTTCTCCTCCATTGACAAAAACACCACCTTATCAATCACCACCTTTTGTGAAACCACCTTCACTTCCACCGTTCACAGCACCAACTCCTCCACCAACTTCACAGCCACCTGTTGCTACAACTCCACCGGCAGCACCTACTCCTATCGTCAAATCATGGAAGGATTGTGTTCCACTATGTGGTTATAGGTGCCAATTACATTCAAGGAAGAACCTATGCTTGAGAGCATGCGTGACATGTTGTAACCGTTGCAAATGTGTCCCTCCAGGAACTTATGGCAACAGAGAAAAGTGTGGAAAATGCTACACTGATATGTTGACTCGTGGCAACAAATACAAGTGTCCATAGATACACAGATTCACACACCTAACTATGAGTTTGAAAAAATGTAATAGTGATTAAGGTTTTAAGTTGCTGGTTTATTAAATAATTTAGGGCCCGGCATTAATTATGCAAGTTAATTAATTACTACTAGTATGTTATATGTATGTGATCTTTGTGGTCCAGAAATTTAACATGGAATTATGGAAGTATATTTTGTTGTTGAAAATCCAGGAGTATAATTAATATGAAGTTATGATCTTGATGAGTTTGATTACTATTTTCTAAGG
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5648
PRIMER_LEFT_PENALTY=0.493142
PRIMER_RIGHT_PENALTY=0.071684
PRIMER_LEFT_SEQUENCE=GTCGGGGAACACTCACTAGATT
PRIMER_RIGHT_SEQUENCE=AGGGTTTGCATAGTTCACCACT
PRIMER_LEFT=0,22
PRIMER_RIGHT=159,22
PRIMER_LEFT_TM=59.507
PRIMER_RIGHT_TM=59.928
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=0.5795
PRIMER_LEFT_1_PENALTY=0.493142
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=GTCGGGGAACACTCACTAGATT
PRIMER_RIGHT_1_SEQUENCE=TTACTGGTGGAGCAAGAACAGA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=190,22
PRIMER_LEFT_1_TM=59.507
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.5795
PRIMER_LEFT_2_PENALTY=0.493142
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=GTCGGGGAACACTCACTAGATT
PRIMER_RIGHT_2_SEQUENCE=TACTGGTGGAGCAAGAACAGAA
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=189,22
PRIMER_LEFT_2_TM=59.507
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.7040
PRIMER_LEFT_3_PENALTY=0.493142
PRIMER_RIGHT_3_PENALTY=0.210886
PRIMER_LEFT_3_SEQUENCE=GTCGGGGAACACTCACTAGATT
PRIMER_RIGHT_3_SEQUENCE=AGCTGAAGGGTTTGCATAGTTC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=165,22
PRIMER_LEFT_3_TM=59.507
PRIMER_RIGHT_3_TM=59.789
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=166
PRIMER_PAIR_PENALTY_4=0.7040
PRIMER_LEFT_4_PENALTY=0.493142
PRIMER_RIGHT_4_PENALTY=0.210886
PRIMER_LEFT_4_SEQUENCE=GTCGGGGAACACTCACTAGATT
PRIMER_RIGHT_4_SEQUENCE=GAGCTGAAGGGTTTGCATAGTT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=166,22
PRIMER_LEFT_4_TM=59.507
PRIMER_RIGHT_4_TM=59.789
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_05041 Ps_contig_mira-and-tgicl-ass_franssen_36207
SEQUENCE=TGCAGAGCTTTTATTATTCGACGAAGATCTTGCTACCAATTTAGTTGGAGGGATTTGTAAGCAAGCTGTGGAGAAATTAGATAAGTTGAGAGCAGCAGCAGCAAGCGTTCTCTACAGAATTTTGTACAACCAGATGATTTATATCCCATATATACCTTTCCGGGAAAAGTTAGAAGAAATAATTTCGAAGGAAGCAGATGCCGAATGGGCTGTTCCTTCGTACACTTACCCGCGCTTCGTACAGCTACTTCAGTTTGGTTGTTATAGCAGAGATGTGCTATCAGGTTTAGTAATATCCGTTGGTGATTTGGCAGATACTGAGAAATCTTTGAAGAGGGTATCACTCTTGACACTGTTAGAGTATTTAGAAGGGATTGAATCTGAAGACCCTAATACAACTACATCCAGGGAGTATATGCTATCAGTTGATATTTTGTGGGTTCTCCAACAATACAGGAAAGACGACAGAGTCATCGTACCCACTTT
TARGET=91,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1278
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.127039
PRIMER_LEFT_SEQUENCE=GTTGGAGGGATTTGTAAGCAAG
PRIMER_RIGHT_SEQUENCE=CCCGGAAAGGTATATATGGGAT
PRIMER_LEFT=43,22
PRIMER_RIGHT=163,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.127
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.1278
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.127039
PRIMER_LEFT_1_SEQUENCE=AGTTGGAGGGATTTGTAAGCAA
PRIMER_RIGHT_1_SEQUENCE=CCCGGAAAGGTATATATGGGAT
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=163,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.127
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=0.1330
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.132262
PRIMER_LEFT_2_SEQUENCE=GTTGGAGGGATTTGTAAGCAAG
PRIMER_RIGHT_2_SEQUENCE=CTCAGTATCTGCCAAATCACCA
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=321,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.1806
PRIMER_LEFT_3_PENALTY=0.053515
PRIMER_RIGHT_3_PENALTY=0.127039
PRIMER_LEFT_3_SEQUENCE=TTGTAAGCAAGCTGTGGAGAAA
PRIMER_RIGHT_3_SEQUENCE=CCCGGAAAGGTATATATGGGAT
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=163,22
PRIMER_LEFT_3_TM=60.054
PRIMER_RIGHT_3_TM=60.127
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=110
PRIMER_PAIR_PENALTY_4=0.1806
PRIMER_LEFT_4_PENALTY=0.053515
PRIMER_RIGHT_4_PENALTY=0.127039
PRIMER_LEFT_4_SEQUENCE=TTTGTAAGCAAGCTGTGGAGAA
PRIMER_RIGHT_4_SEQUENCE=CCCGGAAAGGTATATATGGGAT
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=163,22
PRIMER_LEFT_4_TM=60.054
PRIMER_RIGHT_4_TM=60.127
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=111
=
PRIMER_SEQUENCE_ID=SSR_05042 Ps_contig_mira-and-tgicl-ass_franssen_36208
SEQUENCE=GTTAATTCACAAAACACAATCAAAACAAAGCAATTGTATTTTTTATTTGTAACTTACGTTCAGGGAAAGAAATGGGTTCCTTGAAAGATGAGGATGGGTCAGAATCAGATTCAGATTGTTGAACCTCATTTTGATGCTGCTGCTGCTGATTATACTGATCAGCTTTAGCTTTTTCCAGATAAGGCTTGAAACCAATTCGAAGACTCAAGTAACCAAACGCTACTGCTCCTAACACAACTATCCCTGACCTAATCGCGTTCATCTTCATATCCGATCTCGGAATTCGAGCCATACCCTCTTCAATTCAACAACAAAAAACTCAAACTTTTCTCTCTCAGACTCAAATTAAACGAAGACAGCGGTTCTTGATAAGATTCTTTTGGAGGTAG
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0668
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.006951
PRIMER_LEFT_SEQUENCE=GTTCCTTGAAAGATGAGGATGG
PRIMER_RIGHT_SEQUENCE=ATGAAGATGAACGCGATTAGGT
PRIMER_LEFT=75,22
PRIMER_RIGHT=267,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=193
PRIMER_PAIR_PENALTY_1=0.0668
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=0.006951
PRIMER_LEFT_1_SEQUENCE=GGTTCCTTGAAAGATGAGGATG
PRIMER_RIGHT_1_SEQUENCE=ATGAAGATGAACGCGATTAGGT
PRIMER_LEFT_1=74,22
PRIMER_RIGHT_1=267,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=194
PRIMER_PAIR_PENALTY_2=0.0733
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.013401
PRIMER_LEFT_2_SEQUENCE=GTTCCTTGAAAGATGAGGATGG
PRIMER_RIGHT_2_SEQUENCE=GCGATTAGGTCAGGGATAGTTG
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=181
PRIMER_PAIR_PENALTY_3=0.0733
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.013401
PRIMER_LEFT_3_SEQUENCE=GGTTCCTTGAAAGATGAGGATG
PRIMER_RIGHT_3_SEQUENCE=GCGATTAGGTCAGGGATAGTTG
PRIMER_LEFT_3=74,22
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=59.987
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=0.0940
PRIMER_LEFT_4_PENALTY=0.059875
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=GTTCCTTGAAAGATGAGGATGG
PRIMER_RIGHT_4_SEQUENCE=TTGAATTGAAGAGGGTATGGCT
PRIMER_LEFT_4=75,22
PRIMER_RIGHT_4=307,22
PRIMER_LEFT_4_TM=59.940
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_05043 Ps_contig_mira-and-tgicl-ass_franssen_36220
SEQUENCE=GGTTTTTTCGGCAGAATCCAACCATAACTATATGAAGGGTTAATTACACTGATTGGCATGTCAAATACAGTTCACCTCACTTAAATATAGATTCATAAGGACTGGATCAAGCAAAATCAATAAAACATTTGATATACAAATCTACACGCCACCATTCTAGGTTCTCGCAAATATTTAGAAGGGAAGTTGATACAATAAAATTACAAGTAAATCACAACAGAATAGAGCAAATCAAATCAACAAAAGAACTAAAGGAAGTTAGAATAACTTATCATATTGGCTAGTGAGTGAACTTCTCAGATTTGAATAAAGGCCGTAGAGTGAATATTGGCGAAGGTTTTTCACTTCATACCAAGAATTGAGAGCTGATAGGTGTTTATCAGTGCCAGAGAATGCCAATTGTATACCTAAGCTGCAATCCACAAGTCCTCCATGGCTCTTGCAAGTAGATGTTTGTAGTGCACAATCCTGATTGTTAAGACAGACAGCTTTTGAATTTTTCGGGCATTTAGCACACCCGTCTCTTTTCCAATATAAATTTTGCAGTCTGCCCTTCGTGAACTCAAGAACAAGAGTAAAACTGGTTACAGTATATGAGCCATTTGCAACAAAAGCAGGAGGAGACCTTGCTGCATATTTACGACCAGCAAATGCAACCATATAGCCATACGAGTCCGCAACGAATGAGGAAGAATTAACAGTTAGGAGCGAGATCTCGTCGACCTTAGGTCTGAACAAAGAGATCTGAGAGTTCGAATTGGAGAGAGAGAGTCTAGTATCGCAGGGAGATAACTGAACGCTGTTATTGTTATTGTAGAAGAACTTGTCTTTTGCTGCGAAAGCGAGTCCAAACGTAAAGCCGTCTGATCTCTGCACGCGTGTGTCCGTGCATGGAGAGAATACTTTGTTTGTGTCTCCGGCGTATGCCGGCAATGCCGCCGCCAGAAACGCCACAACGATTGCGGTCGTTAGTGCGCTGATAACCGCCATGGAAGAAGATTCTGGAGGTTTTGCGTTTTGGCCGAGGCAACACGCAGTTGATGATTACAGTGCGAAACTGCTAATTCCCCCYGCYTGCGTTAAGTCCAGGTGTTCT
TARGET=761,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05044 Ps_contig_mira-and-tgicl-ass_franssen_36221
SEQUENCE=GTACTTCATTTTTATTACTCTCTTCTGAATCATCTTCAAATAATGCTTATCTTTATGAATCAAACTCAAAGGTTGAATCTTGGAGGAAATTCAATGGTTTTGATCCGATTCTTAGTGATAACCCTCATCAACAAGGTGTTTACTTTAAAGGAGGTTTGTATTTTGCTACACCAGAGCCTTTTTCTGTTGTGTATTTTGATTTGGAAAATRGGAAAATGGGAGAGGCCTGTTTGTGATTTACCTAATCAACTCACTTTTGTGAGATTGGTTAGTATGAATGATGATGATGATGGTGATTATGATGGTAAAAAACTGTTTTTGATTGGTGGGGTTGGGAGCAATGGAATTTCAAGGAGTATTAAACTATGGGAATTGAGTGAAGAAGGGACTAATTGGGTTGAGATTCAGAGTTTGCCTGATTTGATGTGTAGGAAATTTGTGTCAGTTTGTTATCATAATTATGAACATGTTTATTGTTTTTGGCATGAAGGAATGATTTGTATATGCTGTTATACTTGGCCAGAGATTTTGTATTATTTGGTTTCTAGAAGGACTTGGCATTGGCTTCCTAGGTGTTCTTCTTTGGCTTTGAAATGTAGCTGTGGATTTAAGTGGTTTTCTTTTGTTCCTAAGTTGTATGCTCAAGTTTGAAGGGTGAAAAAGAGTGTAATTGTTTTTTGTAGGTTTTTTGTTTTATGGTTTCTTGTGTTGTAGTAACTTGTATGTAAATGTATCANGTAACAA
TARGET=278,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05045 Ps_contig_mira-and-tgicl-ass_franssen_36224
SEQUENCE=AACCCACCAAGCCCTTTTGATTCCAATGGCACAAAAACAACCTCTAATAGTCAGAGAACAAAAACCAGATGAGAAACTGGTCAAGGTCAATGAGATCCCAATCCAAACTCATTGCTTTCGTTCCCACCATGGGTTACCTTCACCAAGGCCATCTTTCTCTCATCACAGAAGCTCACAAACACGCCAATGTCGTCGCAGTATCAATCTACGTCAACCCGGGTCAGTTTTCACCTAATGAAGATCTTTCCACTTACCCTTCTGATTTTGAAGGTGATGTTCAGAAGCTTTTGTCTGTTCCTGGTGGTGTTGATGTTGTTTTTAATCCAAAGAATTTGTATGATTATGGGGAGAGTGAGGGTGGTGAAGGTGGTGGTGGTGGTGGTGGTGGTGAGGTGGTGTCTTGTGTTGAGAGGAGTGGTTTGGGACATGAGAGTTGGGTTAGAGTTGAGAAACTTGAAAAGGGTCTTTGTGGGAAG
TARGET=366,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1775
PRIMER_LEFT_PENALTY=0.078029
PRIMER_RIGHT_PENALTY=0.099438
PRIMER_LEFT_SEQUENCE=AGAAGCTTTTGTCTGTTCCTGG
PRIMER_RIGHT_SEQUENCE=AACTCTCATGTCCCAAACCACT
PRIMER_LEFT=279,22
PRIMER_RIGHT=434,22
PRIMER_LEFT_TM=59.922
PRIMER_RIGHT_TM=59.901
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=156
PRIMER_PAIR_PENALTY_1=0.2633
PRIMER_LEFT_1_PENALTY=0.078029
PRIMER_RIGHT_1_PENALTY=0.185267
PRIMER_LEFT_1_SEQUENCE=AGAAGCTTTTGTCTGTTCCTGG
PRIMER_RIGHT_1_SEQUENCE=CAAACCACTCCTCTCAACACAA
PRIMER_LEFT_1=279,22
PRIMER_RIGHT_1=421,22
PRIMER_LEFT_1_TM=59.922
PRIMER_RIGHT_1_TM=60.185
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.2749
PRIMER_LEFT_2_PENALTY=0.078029
PRIMER_RIGHT_2_PENALTY=0.196906
PRIMER_LEFT_2_SEQUENCE=AGAAGCTTTTGTCTGTTCCTGG
PRIMER_RIGHT_2_SEQUENCE=TCCTCTCAACACAAGACACCAC
PRIMER_LEFT_2=279,22
PRIMER_RIGHT_2=413,22
PRIMER_LEFT_2_TM=59.922
PRIMER_RIGHT_2_TM=60.197
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=135
PRIMER_PAIR_PENALTY_3=0.3028
PRIMER_LEFT_3_PENALTY=0.203324
PRIMER_RIGHT_3_PENALTY=0.099438
PRIMER_LEFT_3_SEQUENCE=CTGGTGGTGTTGATGTTGTTTT
PRIMER_RIGHT_3_SEQUENCE=AACTCTCATGTCCCAAACCACT
PRIMER_LEFT_3=297,22
PRIMER_RIGHT_3=434,22
PRIMER_LEFT_3_TM=59.797
PRIMER_RIGHT_3_TM=59.901
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=0.3047
PRIMER_LEFT_4_PENALTY=0.205226
PRIMER_RIGHT_4_PENALTY=0.099438
PRIMER_LEFT_4_SEQUENCE=GAAGCTTTTGTCTGTTCCTGGT
PRIMER_RIGHT_4_SEQUENCE=AACTCTCATGTCCCAAACCACT
PRIMER_LEFT_4=280,22
PRIMER_RIGHT_4=434,22
PRIMER_LEFT_4_TM=59.795
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_05046 Ps_contig_mira-and-tgicl-ass_franssen_36229
SEQUENCE=TCCCACCATATTGTTGCCTAATCAATCTTGATGATTTGTCTCACCTTGCTCTTGATATTGGAGGAATACTCTGATAAAGTTGGTGTACTTTTCACGGCATGAAGGCCAATCAGATGRTGATAAAAGGAAGAAAAGTYTAAAAGAGCGGTTAGGGCTTTCTAATGGAAATAGGAGAAGCTTTCCTATTCTTGGTGGAAGGCTTCACTTTGTCAAGTTTGAAACAAGAAAGATCAACGAGTGCTTAGATTTCATTCATTCAAAGCAGCTTCACCGAGGGTTGGAGTCACGTTACTCAGATGCTGCGGCTAATGAAAATGCCATAATTAAGGCTACTGGAGGTGGTGCATACAAGTATACTGACCTTTTCAAAGAAAGACTTGGGGTTGGTCTTGACAAAGAAGATGAAATGAATTGTCTTGTGGCAGGAGCGAATTTCTTGCTTAAGGCAATTCGACATGAAGCTTTCACACACATGGAGGGCAAGAAAGAGTTTGTTCAAATTGACCATAATGATTTGTTCCCTTATCTTCTAGTTAACGTTGGTTCTGGTGTTAGTATGATCAAGGTTGATGGGGATGGAAAGTTTGAGGAGGGTTAGTGGGAACAAATTGGTTGGTGGTGTTACTTAGTGGGGCTTAGGG
TARGET=248,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05047 Ps_contig_mira-and-tgicl-ass_franssen_36244
SEQUENCE=CCTCACGTTCCTCTCTCNTTAAAGAACACTCACTCNCAACTCTCATTAGAGACCACACACTCCTCCATCACTCTCTCAGTTCATTAAAAATAAAAAGAAAAATCCATTTCCTCTCCCTTCGCCTCTCACCAAAACCAAAATCACCGCCTGAATCTCCTTCACGCACACTCATCCTCACCGCGCCGTAAACCTCCATCACCACCGTCCGGTCGTTCAACCTCTCATCTCCGTCATGACAACCGACATCGAAACCCTCTTCTCCTTCAACCACCTTCATCATCTCCCGCTAAACCATCACCGTCGTCACGTCTAACGACCATCACCTCCGAACGCCGGTAACGCGACCTTTACCGTCATCAAAGGCTCGCCGGTGACTCACAAACCACCACCACATCACATACCGCCATGGAAGAATGTAACCACCGAACCGCCGCGTTCTCCGACCTCACCGTAAACCTTCACCTATGGTACAACCCAAATAAACCCCTCTGTTCTCAGCTTCTTCTAACCACAAACATCTCCATTCACCAAACACTCACACAATAACCTTGCTTTTGTTTCCGAACTCAGTCGCGCCACCAACGGCATCGAATCTGCATCTCACGTTGTCAACCCCTCTTCAATGGCAAGCTACTCCATCTTCATTGTCATTATTGTGCGTAATGATGCTCAACGTCTCGAGCTCAATACAGAAAGAAATTGGATCTGCAGCAAGATCAATGTCACCCATCGAGGTTTAAGTGGTGTTTTTCAATTTGATTATCTCTGTTTATCATGTTCTAATTTATTTTTGATTCGCTGTTTTGCACGTATCATGTCGACATCCTCCGTTTCATTCTTTTAGTGTTTGATATGTATGTCACTGGTTTAAGAGTGTTGTTGTTGTTTTCAATATGTTATGATTCTAAAATGTATGTCGGTTTTGCCATGTATCATTCCAATCTTGTTAGGTGCTTCTGTTGACCTAAGGGGTTGATAATTATTTGGGTCAACTCAGTTTGGTTAGTGTTTGTATTGATGTGCTTATTTCAGTCATCTATTGCAGTCTGATTATTCCATGTTTGGGCTGGATTTTGATGCTATAATATTGATGGTGTGATACTTATGCAATGTTGTGAATGTATTTTATGTTGATGTTAGTTTGACTTGCATACAAATTACAATCACCCTGTTGTGAT
TARGET=875,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3776
PRIMER_LEFT_PENALTY=0.241544
PRIMER_RIGHT_PENALTY=0.136017
PRIMER_LEFT_SEQUENCE=ATGTCACCCATCGAGGTTTAAG
PRIMER_RIGHT_SEQUENCE=ATCAACCCCTTAGGTCAACAGA
PRIMER_LEFT=719,22
PRIMER_RIGHT=976,22
PRIMER_LEFT_TM=60.242
PRIMER_RIGHT_TM=59.864
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.4108
PRIMER_LEFT_1_PENALTY=0.274768
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=CCATCGAGGTTTAAGTGGTGTT
PRIMER_RIGHT_1_SEQUENCE=ATCAACCCCTTAGGTCAACAGA
PRIMER_LEFT_1=726,22
PRIMER_RIGHT_1=976,22
PRIMER_LEFT_1_TM=60.275
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=0.4311
PRIMER_LEFT_2_PENALTY=0.241544
PRIMER_RIGHT_2_PENALTY=0.189529
PRIMER_LEFT_2_SEQUENCE=ATGTCACCCATCGAGGTTTAAG
PRIMER_RIGHT_2_SEQUENCE=CCTTAGGTCAACAGAAGCACCT
PRIMER_LEFT_2=719,22
PRIMER_RIGHT_2=969,22
PRIMER_LEFT_2_TM=60.242
PRIMER_RIGHT_2_TM=59.810
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.4643
PRIMER_LEFT_3_PENALTY=0.274768
PRIMER_RIGHT_3_PENALTY=0.189529
PRIMER_LEFT_3_SEQUENCE=CCATCGAGGTTTAAGTGGTGTT
PRIMER_RIGHT_3_SEQUENCE=CCTTAGGTCAACAGAAGCACCT
PRIMER_LEFT_3=726,22
PRIMER_RIGHT_3=969,22
PRIMER_LEFT_3_TM=60.275
PRIMER_RIGHT_3_TM=59.810
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.4685
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=0.226971
PRIMER_LEFT_4_SEQUENCE=ATGTCACCCATCGAGGTTTAAG
PRIMER_RIGHT_4_SEQUENCE=ACCAAACTGAGTTGACCCAAAT
PRIMER_LEFT_4=719,22
PRIMER_RIGHT_4=1002,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=59.773
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_05048 Ps_contig_mira-and-tgicl-ass_franssen_36255
SEQUENCE=AATTGCACACACATGGATAGATAGATAGAGCTCTGATTCTCATCTCATTTGGATAGAATAGCTGTTGAAGAACTGACTTAAACAAGTGAAGATTGCGGGCGTAACCGTTCTTCTTTTTCTCCTCTTATTGTAGCTTCCAGAACTTATCACTTACTTAACGGCATGATTGACAGCGCGTAACACCCACTCGCCACTCCCATGACGGTTGTTTCACTCTTCCTCACCGCCACCAAACTCGCCGGAGCCTTAGTCACTCTCTCCCTCGCTGCCAATGTCTTCTCCTTCTCTCGTTTTCGCAACAAAAACCTTCGTCCGTTAGGTTCCCCTATCGATGAGTCCTCCGATACTCTCGCCGATTTCAACATTACCGAAGGAGAAAACGAGTTTTTCTTTGGTCTGGCAACAGCACCGGCACATGTAGAGGACAGGCTTGATGATGCTTGGATTCAGTTTGCTGAACAAGAGAGTATTGCCGGTGCTGAGCAGAAAGTGGATGCTTTGATGGGTYCTGCTACTGCTGATGGTGGTTCTCAACAAGCTGTTTCATCTCCACAACGTGCTAGGAAGGGAAACAAGAAGTCTCTTAAGGTTGCTATGGAGGCCATGATCAGAGGATTTGAGAAAT
TARGET=16,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05049 Ps_contig_mira-and-tgicl-ass_franssen_36256
SEQUENCE=AAAGAATAGTTTTTCTACAAAAGGCTTCGTTATTAAGAGGCTGACATCCTTTCTTGTAGTCTGTAAGTAGCTGTAATTATGAGCAATTGATGATTAGTGAACGTTGCTTAGATTTAAAATTAGGCTTAATTCTTTTTTCCGTCAGCCTTTACATTGTTTTTATTTTTTAATCATCATGTAGCTGATTTTATTTAGTGGGATAAAACTTGGTTGTTGTTGTTGTANNAAGTGTATCAAAATTATTAAGCATTTTGTCC
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05050 Ps_contig_mira-and-tgicl-ass_franssen_36271
SEQUENCE=AAGCCACAACTATGAGCTTCAATTTGCAAAATCTCCTAATCAACCTAACCAAGTTCAAAAACTGTTGCGCCTTCATTAGTAACAAAACAATTATAGTAATAATGATGATGATGAGGCATATCTAGATTGCTGGGAAGAAGAACACGTGTACCTCCATGTATGAGCTTTGTGGAGCGAGAAGAAGTTCCAGAAGAGAAATCCTCTCTTTCAACGAGGCCAACACGGAGTCCACGTGTGGCGGCATCGAAGGGACCG
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2431
PRIMER_LEFT_PENALTY=0.189072
PRIMER_RIGHT_PENALTY=0.054021
PRIMER_LEFT_SEQUENCE=AAACTGTTGCGCCTTCATTAGT
PRIMER_RIGHT_SEQUENCE=AACTTCTTCTCGCTCCACAAAG
PRIMER_LEFT=58,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=60.189
PRIMER_RIGHT_TM=60.054
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=0.4160
PRIMER_LEFT_1_PENALTY=0.189072
PRIMER_RIGHT_1_PENALTY=0.226971
PRIMER_LEFT_1_SEQUENCE=AAACTGTTGCGCCTTCATTAGT
PRIMER_RIGHT_1_SEQUENCE=CTTCTGGAACTTCTTCTCGCTC
PRIMER_LEFT_1=58,22
PRIMER_RIGHT_1=192,22
PRIMER_LEFT_1_TM=60.189
PRIMER_RIGHT_1_TM=59.773
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=0.4160
PRIMER_LEFT_2_PENALTY=0.189072
PRIMER_RIGHT_2_PENALTY=0.226971
PRIMER_LEFT_2_SEQUENCE=AAACTGTTGCGCCTTCATTAGT
PRIMER_RIGHT_2_SEQUENCE=TCTTCTGGAACTTCTTCTCGCT
PRIMER_LEFT_2=58,22
PRIMER_RIGHT_2=193,22
PRIMER_LEFT_2_TM=60.189
PRIMER_RIGHT_2_TM=59.773
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.4160
PRIMER_LEFT_3_PENALTY=0.189072
PRIMER_RIGHT_3_PENALTY=0.226971
PRIMER_LEFT_3_SEQUENCE=AAACTGTTGCGCCTTCATTAGT
PRIMER_RIGHT_3_SEQUENCE=CTCTTCTGGAACTTCTTCTCGC
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=194,22
PRIMER_LEFT_3_TM=60.189
PRIMER_RIGHT_3_TM=59.773
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=137
PRIMER_PAIR_PENALTY_4=0.6972
PRIMER_LEFT_4_PENALTY=0.643136
PRIMER_RIGHT_4_PENALTY=0.054021
PRIMER_LEFT_4_SEQUENCE=AAAACTGTTGCGCCTTCATTAG
PRIMER_RIGHT_4_SEQUENCE=AACTTCTTCTCGCTCCACAAAG
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=60.643
PRIMER_RIGHT_4_TM=60.054
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=129
=
PRIMER_SEQUENCE_ID=SSR_05051 Ps_contig_mira-and-tgicl-ass_franssen_36274
SEQUENCE=TTGTAGATTTAGACTGTCTCTTGTGCATGAAGCCAACTTATTTTTCAAGCTCTCAATCTCCTGTTCCAAGCCAGCTGTCCTTTGAAAATGCATTCGGGTAGCGATAGCAAGATAGGCAGGCCCAGCTTGCTGCATGCATAGCTGTTCAATATCTTTGCGTAATTCATCACGTTCATGTTCCAGTACTCGAATTCGTGCCAGCAAAGAATTACAATCATTGACTACCCCTCAGCCTATTCTAGGAAATTCGATATATCAACAACAACAACAACAACAACAACAAACGAAAAGG
TARGET=257,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05052 Ps_contig_mira-and-tgicl-ass_franssen_36285
SEQUENCE=CGACGAGAGAGAGCTGATGCATTTTGTGAGGCAATCCGTGCTCTGGATGCTACAGATCTTCCTGCAAATAGTGTTAGGGACTTCTTTCTGCCTGCAATACAGAACCTCTTGAAAGACTTRGATGCACTGGATCCAGCTCACAAAGAAGCACTTGAAATTATTATGAAGGAAAGATCAGGGGGAACTTTTGAGACTATTAGCAAGGTAATGGGTGCTGCTCATCTTGCGCTTCCAACATCAGTTAGCAACATTTTTGGTGAGGGTGGGTTGCTTGGAAAGAAAGAAACCACTGAGTCACCAACAGAGGCAGTTCTGTCCCCCAAGAGTGTCACTCCACCAGCAGAGGACACTAGATTCAGACGTATCATGATGGGAAATTTCAGCGAGATGCTTCGTGGCAAAGCAAAAGCCCAAGAGGATGGTCAGAACCAATAAGATGCCTTTGGCCTTAGCAATATTGTGCTTAAGCTGTTTCATCAAGAGAATGTCACTGTTTCCCATATGTACAACAACCAAGACTTGTAGTAATTTTCTTTTACAGTTGTTGTTTATGAGATARRAAAAGAGTAGAAAATATAAATAAATAGTTTATTACAGTTTTTGATGCCAAAATTTGTGGTTGTGATCTAATAGGCCTTGTGTCGGGACAGGAAAGAGATTTTGGTCGAGAGCACATTGTAAATGTGAATTGTTCATTAAATTTTCATCTGCATGCTTTGTAGT
TARGET=275,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05053 Ps_contig_mira-and-tgicl-ass_franssen_36323
SEQUENCE=CAGAGTACGCGGGGACGTTATCTTCACCTATATATTCCCACATCAAACCGAAAGCACGACTTACTTAAAATGCACATTCTTCTAAAACTCCTTTTTTGGTTAAGCATTCTCTACATCAATATGGCATCTTCTTCTTCTCTCTCATCAGATACCAAACAGCAAGGATTTGTTTGTGGTATTGAGGATAAGGAACAAAATTCAGTTCATGAAAATGTTCTTCAGAAACATGCTGCTTTCTTTGATGTCAATAAGGATGGTCTTATTTATCCGTGGGAAACTTTCCGAGCGATGCGTGAAATCGGGAGTGGAGTGTTGTTATCAACTGCAGCTGCTGTTTTCATCAATATTTCCCTCAGTCAGACTACTCGTCCGGGAAAGTTTCCATCTCCACTTTTTCCAATTGAAGTTAAGAATATCAAACTAGGCAAACATGGCAGTGACAGTGGAGCTTATGACAGTGAAGGAAGGTTTGTTGAATCAAAATTTGAAGAAATTTTCATGAAACATGCACATACTCATCCAAATGCTTTAACACATGATGAGCTCGACGAGTTGATAAAGGCAAATAGAGAACCTAAAGATATCAAAGGAAGGATTGGTGGCTTTGTTGAATGGAAAATTCTTTACAAACTTGCGAAAGACAAGAATGGTTTACTTCCCAAAGAAACAATTCGTGGTGTTTATGATGGAAGCTTGTTTGAAGTGCTCAAAAAGGAACATGCAAAAAGAAAAAGTTCTTCCTAATATTATGAGTCATAATATGTTAATTTATCTGTATAGACAAATATACAAAAGTTTGTTTATACAATTGATGCTCCGTATATAATTTACAAAGTATGTCAATTTGTTTTGGTTTCATCCTTCATTTATTTGTGTTAAGTAATGTATCTGCATGACAATTTATATATATGTTCCCGAGTGATTTTGYATTTGTTATATTTATTTTA
TARGET=127,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05054 Ps_contig_mira-and-tgicl-ass_franssen_36341
SEQUENCE=AGTTGCACGTTTCTTCATGATGAGCAATCCAAGAATCGCGAAAGTGTTGCTATAAGTTTAGGTCCTGGTGGTTATGGAGGTGGTGGTGGAGGTAGTACTGGTAGTGGTGGTGGTGGTGGTGGTGGTGGTGGAGGTAGTGCTAGTGGTGTTGTTGTTGTTGCGGCTGGTGCTGCAGCGGCTGGAAATGGAACAAATTTGAAGCCTTCGAATTGGAAAACTAGGATTTGCAATAAGTGGGAAATGACTGGGTATTGCCCCTTTGGTAACAAATGCCATT
TARGET=104,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3219
PRIMER_LEFT_PENALTY=0.213506
PRIMER_RIGHT_PENALTY=0.108385
PRIMER_LEFT_SEQUENCE=CTTCATGATGAGCAATCCAAGA
PRIMER_RIGHT_SEQUENCE=TTTGTTACCAAAGGGGCAATAC
PRIMER_LEFT=12,22
PRIMER_RIGHT=269,22
PRIMER_LEFT_TM=60.214
PRIMER_RIGHT_TM=60.108
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.3219
PRIMER_LEFT_1_PENALTY=0.213506
PRIMER_RIGHT_1_PENALTY=0.108385
PRIMER_LEFT_1_SEQUENCE=TCTTCATGATGAGCAATCCAAG
PRIMER_RIGHT_1_SEQUENCE=TTTGTTACCAAAGGGGCAATAC
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=269,22
PRIMER_LEFT_1_TM=60.214
PRIMER_RIGHT_1_TM=60.108
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.3230
PRIMER_LEFT_2_PENALTY=0.213506
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=CTTCATGATGAGCAATCCAAGA
PRIMER_RIGHT_2_SEQUENCE=CAATACCCAGTCATTTCCCACT
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=253,22
PRIMER_LEFT_2_TM=60.214
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.3230
PRIMER_LEFT_3_PENALTY=0.213506
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=TCTTCATGATGAGCAATCCAAG
PRIMER_RIGHT_3_SEQUENCE=CAATACCCAGTCATTTCCCACT
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=60.214
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.3425
PRIMER_LEFT_4_PENALTY=0.234069
PRIMER_RIGHT_4_PENALTY=0.108385
PRIMER_LEFT_4_SEQUENCE=TTAGGTCCTGGTGGTTATGGAG
PRIMER_RIGHT_4_SEQUENCE=TTTGTTACCAAAGGGGCAATAC
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=269,22
PRIMER_LEFT_4_TM=60.234
PRIMER_RIGHT_4_TM=60.108
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_05055 Ps_contig_mira-and-tgicl-ass_franssen_36341
SEQUENCE=AGTTGCACGTTTCTTCATGATGAGCAATCCAAGAATCGCGAAAGTGTTGCTATAAGTTTAGGTCCTGGTGGTTATGGAGGTGGTGGTGGAGGTAGTACTGGTAGTGGTGGTGGTGGTGGTGGTGGTGGTGGAGGTAGTGCTAGTGGTGTTGTTGTTGTTGCGGCTGGTGCTGCAGCGGCTGGAAATGGAACAAATTTGAAGCCTTCGAATTGGAAAACTAGGATTTGCAATAAGTGGGAAATGACTGGGTATTGCCCCTTTGGTAACAAATGCCATT
TARGET=147,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3219
PRIMER_LEFT_PENALTY=0.213506
PRIMER_RIGHT_PENALTY=0.108385
PRIMER_LEFT_SEQUENCE=CTTCATGATGAGCAATCCAAGA
PRIMER_RIGHT_SEQUENCE=TTTGTTACCAAAGGGGCAATAC
PRIMER_LEFT=12,22
PRIMER_RIGHT=269,22
PRIMER_LEFT_TM=60.214
PRIMER_RIGHT_TM=60.108
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.3219
PRIMER_LEFT_1_PENALTY=0.213506
PRIMER_RIGHT_1_PENALTY=0.108385
PRIMER_LEFT_1_SEQUENCE=TCTTCATGATGAGCAATCCAAG
PRIMER_RIGHT_1_SEQUENCE=TTTGTTACCAAAGGGGCAATAC
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=269,22
PRIMER_LEFT_1_TM=60.214
PRIMER_RIGHT_1_TM=60.108
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.3230
PRIMER_LEFT_2_PENALTY=0.213506
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=CTTCATGATGAGCAATCCAAGA
PRIMER_RIGHT_2_SEQUENCE=CAATACCCAGTCATTTCCCACT
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=253,22
PRIMER_LEFT_2_TM=60.214
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.3230
PRIMER_LEFT_3_PENALTY=0.213506
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=TCTTCATGATGAGCAATCCAAG
PRIMER_RIGHT_3_SEQUENCE=CAATACCCAGTCATTTCCCACT
PRIMER_LEFT_3=11,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=60.214
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.3425
PRIMER_LEFT_4_PENALTY=0.234069
PRIMER_RIGHT_4_PENALTY=0.108385
PRIMER_LEFT_4_SEQUENCE=TTAGGTCCTGGTGGTTATGGAG
PRIMER_RIGHT_4_SEQUENCE=TTTGTTACCAAAGGGGCAATAC
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=269,22
PRIMER_LEFT_4_TM=60.234
PRIMER_RIGHT_4_TM=60.108
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_05056 Ps_contig_mira-and-tgicl-ass_franssen_36344
SEQUENCE=ACGAGTGAGGTAATTTATCATTCATTCATCACATGAAATGGCGCTAAGATTGAGTAGCACTTTCTTTTCCGTTTCTGTTATAGCTTCAAATCATTGTTCAACAAAACCCACTTTCAGATTCAAAATCACAACTCTTGCTGCTACAAATTCAATTACAATGGCTTTCACTCAAACCCCAAATTCAACTTCAACTTCCAAAATCATTGATTCTCATCTCCACGTATGGGCTTCCCTTCAAGAGGCTAGTAAATTTCCTTACTCTCCTGGACAAGAGCCCAATTTACCCGGAAATGTTGATTTTTTGCTTCAGTGTATGGAGGAAGCRGGAGTAGATGGTGCACTCATTGTACAGCCAATTAATCATAGATTTGATCATAGTTATGTCACAAGTGTTTTGAAGAAATATCCGAACAAATTTGTCGGTTGTTGTCTTGCCAATCCAGCTGATGATGGAAGTGGGCTTAAGGAGTTTGAACATCTTGTTCTCAAGGATGGCTACCGCGCTGTTCGATTCAACCCTTATTTATGGCCATCTGGTGAAAAGATGACAAATGAAATAGGGAAAGCAATTTTCAAAAGGGCTGGTGAACTCAATGTATCAGTGGGCTTCATGTGTATGAAGGGGCTTGATCTGCATATTTCTGAAATTGAGGAACTGTGCACAGAATTTCCATCAACAGTTGTATTGCTCGATCACTTGGCCTTCTGCAAACCACCATTAAATGATGAAGAAGGTCTTGTGTTTTCCAAGCTTTTAAATTTATCTAGATTCCCTCAAGTATATGTGAAATTCAGTGCACTCTTCAGAGTATCAAGGACACAATTTCCTTATCTGGATTTATCTCCTCTCTTGTCCCAAATAGTCTCTAGCTTTGGTGCCAACCATGTAATATGGGGCAGTGATTTTCCATTTGTTGTTCCTGAATGTGGCTACAAAGAAGCCAAAGAAGCAGTGAAACTTATTGGCAATCAAATATCTTTGCCTTCATCTGATTTAGAATGGATCATGGGTAAGACTGCTGCACAACTTTTCCAAAGCCAATTGACTGCTTCTGAGTAAGATTTTTGTTGGGGGTGAGAATGCTGTCTCTGGTTGATCATTACAAAACACTTTTAACACGCCTCAGGCTTCCGACATTAATCGCGTGAATGGTCCTACTTTCACTACAACTTAATAGAAGCTCCAAACCAAACTTTTACAGCATTGTTGTGTTCCACTGTTGATTTGGTGTAATCATGTAATATACATTTTATTTTATATACTAGTAGTTCTTTAGAATAAGATTTGCTATCAAGAGTAATTAAAGAAAAGGCAAACATTTATAAAAGACAAACTTACATTGATAGG
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05057 Ps_contig_mira-and-tgicl-ass_franssen_36351
SEQUENCE=TCCGGCNCTCATCAGAACTTTCATAAAATAAANACTAACAAAGGTCCAACAATANCCAATTCCATAGTGCATCCTCAATGCAAGGCCATATGCAACCTCTCATGTTTCACAGCTCTTAACCAGAAATTGGAAAGAAAAAGGGAAAAAAAACTTAACAAATGGTCCAACAATACTCCAAATTCCATAATACTACCTCGCACACCACCTTGCTTGCTTGAGTGACAGACACATTAACCATAGCTACTCATCAAACTAGTAAAACAACCCATGTAACTACTGTGGTCACCGGCAATAAAATGCTTGATCAATGTGTGGCGGAATTTGCTTTATTTCAGTGCCAAGTTCTTGTTCAATCCTATATAAATTAAAGCGGTCCTCGTAAGTGATCAAGTTCACCGCTAAACCAAGGTGGCCAAATCTCCCTGAACGACCAACCCTGTGCAAATAAGTTTCCGAGTTCTTGGGAAAATCAAAGTTTATAACAACATTAACTGCTTGGATGTCTATTCCCCTAGTAAAAAGATCAGTACAGACAAGATTTCGGCATGCACCATTACGGAAATCATGAAATACTCTATTACGATGGTCTTGCAGCATCTTTGCATGAATATAGAAACATGAATACCCCAGTTCAGTGATTTTCTTGGCAAGCAGTTCAACCCGATTCACTGAGTTGCAGAAGATAATTGACTGGTTTATTTGTAGCTTAGAAAAGAGAGTATTTAAGCAGTGAACTTTCTGTCTCTCTTCCACAAATGCATAAAATTGTGTAATACCCTTCAGCGTGAGCTCATCCATAAGGTTAATGACATATGGTTTGTTAAGAAACCTATCCTTGAAGTCCTTCACAGTAACAGGATATGTTGCTGAAAACATC
TARGET=206,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4249
PRIMER_LEFT_PENALTY=0.275749
PRIMER_RIGHT_PENALTY=0.149184
PRIMER_LEFT_SEQUENCE=CAAATGGTCCAACAATACTCCA
PRIMER_RIGHT_SEQUENCE=ATCACTTACGAGGACCGCTTTA
PRIMER_LEFT=155,22
PRIMER_RIGHT=386,22
PRIMER_LEFT_TM=59.724
PRIMER_RIGHT_TM=60.149
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.4439
PRIMER_LEFT_1_PENALTY=0.275749
PRIMER_RIGHT_1_PENALTY=0.168114
PRIMER_LEFT_1_SEQUENCE=CAAATGGTCCAACAATACTCCA
PRIMER_RIGHT_1_SEQUENCE=CGGTGAACTTGATCACTTACGA
PRIMER_LEFT_1=155,22
PRIMER_RIGHT_1=397,22
PRIMER_LEFT_1_TM=59.724
PRIMER_RIGHT_1_TM=60.168
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.4713
PRIMER_LEFT_2_PENALTY=0.275749
PRIMER_RIGHT_2_PENALTY=0.195574
PRIMER_LEFT_2_SEQUENCE=CAAATGGTCCAACAATACTCCA
PRIMER_RIGHT_2_SEQUENCE=AACTTATTTGCACAGGGTTGGT
PRIMER_LEFT_2=155,22
PRIMER_RIGHT_2=450,22
PRIMER_LEFT_2_TM=59.724
PRIMER_RIGHT_2_TM=59.804
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=0.5446
PRIMER_LEFT_3_PENALTY=0.275749
PRIMER_RIGHT_3_PENALTY=0.268895
PRIMER_LEFT_3_SEQUENCE=CAAATGGTCCAACAATACTCCA
PRIMER_RIGHT_3_SEQUENCE=AAACTTATTTGCACAGGGTTGG
PRIMER_LEFT_3=155,22
PRIMER_RIGHT_3=451,22
PRIMER_LEFT_3_TM=59.724
PRIMER_RIGHT_3_TM=60.269
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.5581
PRIMER_LEFT_4_PENALTY=0.275749
PRIMER_RIGHT_4_PENALTY=0.282356
PRIMER_LEFT_4_SEQUENCE=CAAATGGTCCAACAATACTCCA
PRIMER_RIGHT_4_SEQUENCE=ACTTATTTGCACAGGGTTGGTC
PRIMER_LEFT_4=155,22
PRIMER_RIGHT_4=449,22
PRIMER_LEFT_4_TM=59.724
PRIMER_RIGHT_4_TM=60.282
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_05058 Ps_contig_mira-and-tgicl-ass_franssen_36358
SEQUENCE=AATTAACCAATTCAAAACAAAGTAGCTGAAACTTTCAATGGGATCTAATTCTTCCAGCATGAAGCTGCCTGGAAGCAGTCCACAGCATCACCGATTCGGCCATCGTGCTTGTGAACCAATCCCAAGTAANACCAAGCCATCCGGTTGGTTGGTTCTATTCTAAGCGCATCAGACAGCATGCTTCTTGCAGCAGGCAATGCCTTTGTACCCATTTTATGAATCAAAGCACTCATTAAGATTTTGCTAGGAATGTGGTTGGGTTCAATTAGTATAGCATTAATAGAAGCATTGAGAGCGTCTATATTCTGGCCGCGTCCTTCGAACAAAATACCTTCAGTGTGCAAAATTGCTGCAGAATACTCCTTCAATTCCCTGGCCTCTTGCAAACAGATTTCAGCATCCTTCAAATGAGAAAGACTGGCATACAAATTTGCAAGACCGTGA
TARGET=143,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4118
PRIMER_LEFT_PENALTY=0.069526
PRIMER_RIGHT_PENALTY=0.342304
PRIMER_LEFT_SEQUENCE=ATGGGATCTAATTCTTCCAGCA
PRIMER_RIGHT_SEQUENCE=GCTGTCTGATGCGCTTAGAATA
PRIMER_LEFT=37,22
PRIMER_RIGHT=176,22
PRIMER_LEFT_TM=59.930
PRIMER_RIGHT_TM=59.658
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=140
PRIMER_PAIR_PENALTY_1=0.4228
PRIMER_LEFT_1_PENALTY=0.249969
PRIMER_RIGHT_1_PENALTY=0.172839
PRIMER_LEFT_1_SEQUENCE=GAACCAATCCCAAGTAANACCA
PRIMER_RIGHT_1_SEQUENCE=GCAATTTTGCACACTGAAGGTA
PRIMER_LEFT_1=112,22
PRIMER_RIGHT_1=349,22
PRIMER_LEFT_1_TM=59.750
PRIMER_RIGHT_1_TM=60.173
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.50
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.8750
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.4228
PRIMER_LEFT_2_PENALTY=0.249969
PRIMER_RIGHT_2_PENALTY=0.172839
PRIMER_LEFT_2_SEQUENCE=TGAACCAATCCCAAGTAANACC
PRIMER_RIGHT_2_SEQUENCE=GCAATTTTGCACACTGAAGGTA
PRIMER_LEFT_2=111,22
PRIMER_RIGHT_2=349,22
PRIMER_LEFT_2_TM=59.750
PRIMER_RIGHT_2_TM=60.173
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.50
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.50
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5500
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.5301
PRIMER_LEFT_3_PENALTY=0.069526
PRIMER_RIGHT_3_PENALTY=0.460533
PRIMER_LEFT_3_SEQUENCE=ATGGGATCTAATTCTTCCAGCA
PRIMER_RIGHT_3_SEQUENCE=AAGGTATTTTGTTCGAAGGACG
PRIMER_LEFT_3=37,22
PRIMER_RIGHT_3=333,22
PRIMER_LEFT_3_TM=59.930
PRIMER_RIGHT_3_TM=59.539
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.5301
PRIMER_LEFT_4_PENALTY=0.069526
PRIMER_RIGHT_4_PENALTY=0.460533
PRIMER_LEFT_4_SEQUENCE=TGGGATCTAATTCTTCCAGCAT
PRIMER_RIGHT_4_SEQUENCE=AAGGTATTTTGTTCGAAGGACG
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=333,22
PRIMER_LEFT_4_TM=59.930
PRIMER_RIGHT_4_TM=59.539
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_05059 Ps_contig_mira-and-tgicl-ass_franssen_36377
SEQUENCE=GTCAAAAGACAAGCGTATAGAATGTAATCTAGAGAAGTGTTCAGGGCATATCAAAGACAAATCTGTATTACAACAATATCATCCTCTCATATCAACAGTCAAATATTTGTCAATAATTTAAGACCACAACTAGGCATCATCAATGCCATTCTCTAACAAATAATACTGTGATTTGCAATGTCAAACATATAATAATAAAGCACAAAAATGGTTCGGATGAGCATGAACTAATAAGGTCGGCCATACTGTTTAGTCATATTATTTACAGCTTCTCCAGGTGGGGGTCTTGGTCTTGCATACTCTGCAAATATAACCCAGCCATCCAAAAACTTGCCATCCATGCCTTCAATGCCTTTAGCAGCATCTTCTAAAGTGGCATACTGCACAAAACCAAACCCCTTGGAGTAACCTGAGACTCGATCAGTTACCACCCTTGCATGAACCACTTCGCCAAACTTTTGAAACTCTTCTCGCAGTCTCTCCGTTGTAGTACGTTTGCTAAGCCCAGACACGAAGAGGTTGGTGTTGGGATCCGCCATCTGTCGAGCGGAAGTAGCTCCGGAAGGTGGCGTTGAAGCGAAAGGGAAAGTGGGAGAAGACGGGGTTGGGTTCGAGCAGAAAAAGCGGCGCAAACCGCGGGGCGCCGCCGCCGCTGCAGCTCTCATCGTCAGAGCCATGGGTTTAAGCTTTGAAATGTAAAACCCTTGGTAGGTTGAAGGAGAGAGAGAAGAAAGACGAATAACNGAAACCGCCTATTGCTGATTTTTATATTTATATTAACAACAAAAACACTGGTGGGTGGGTACTGATCCACCGG
TARGET=642,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1384
PRIMER_LEFT_PENALTY=0.073989
PRIMER_RIGHT_PENALTY=0.064372
PRIMER_LEFT_SEQUENCE=TCGCAGTCTCTCCGTTGTAGTA
PRIMER_RIGHT_SEQUENCE=CGGTTTCNGTTATTCGTCTTTC
PRIMER_LEFT=470,22
PRIMER_RIGHT=750,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=4.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.2182
PRIMER_LEFT_1_PENALTY=0.153836
PRIMER_RIGHT_1_PENALTY=0.064372
PRIMER_LEFT_1_SEQUENCE=AGTACGTTTGCTAAGCCCAGAC
PRIMER_RIGHT_1_SEQUENCE=CGGTTTCNGTTATTCGTCTTTC
PRIMER_LEFT_1=488,22
PRIMER_RIGHT_1=750,22
PRIMER_LEFT_1_TM=59.846
PRIMER_RIGHT_1_TM=59.936
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.2273
PRIMER_LEFT_2_PENALTY=0.162964
PRIMER_RIGHT_2_PENALTY=0.064372
PRIMER_LEFT_2_SEQUENCE=TGTAGTACGTTTGCTAAGCCCA
PRIMER_RIGHT_2_SEQUENCE=CGGTTTCNGTTATTCGTCTTTC
PRIMER_LEFT_2=485,22
PRIMER_RIGHT_2=750,22
PRIMER_LEFT_2_TM=59.837
PRIMER_RIGHT_2_TM=59.936
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.2716
PRIMER_LEFT_3_PENALTY=0.073989
PRIMER_RIGHT_3_PENALTY=0.197640
PRIMER_LEFT_3_SEQUENCE=TCGCAGTCTCTCCGTTGTAGTA
PRIMER_RIGHT_3_SEQUENCE=CAATAGGCGGTTTCNGTTATTC
PRIMER_LEFT_3=470,22
PRIMER_RIGHT_3=757,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.802
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.50
PRIMER_LEFT_3_END_STABILITY=4.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.3286
PRIMER_LEFT_4_PENALTY=0.073989
PRIMER_RIGHT_4_PENALTY=0.254567
PRIMER_LEFT_4_SEQUENCE=TCGCAGTCTCTCCGTTGTAGTA
PRIMER_RIGHT_4_SEQUENCE=TCTCTCCTTCAACCTACCAAGG
PRIMER_LEFT_4=470,22
PRIMER_RIGHT_4=723,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=59.745
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=4.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_05060 Ps_contig_mira-and-tgicl-ass_franssen_36377
SEQUENCE=GTCAAAAGACAAGCGTATAGAATGTAATCTAGAGAAGTGTTCAGGGCATATCAAAGACAAATCTGTATTACAACAATATCATCCTCTCATATCAACAGTCAAATATTTGTCAATAATTTAAGACCACAACTAGGCATCATCAATGCCATTCTCTAACAAATAATACTGTGATTTGCAATGTCAAACATATAATAATAAAGCACAAAAATGGTTCGGATGAGCATGAACTAATAAGGTCGGCCATACTGTTTAGTCATATTATTTACAGCTTCTCCAGGTGGGGGTCTTGGTCTTGCATACTCTGCAAATATAACCCAGCCATCCAAAAACTTGCCATCCATGCCTTCAATGCCTTTAGCAGCATCTTCTAAAGTGGCATACTGCACAAAACCAAACCCCTTGGAGTAACCTGAGACTCGATCAGTTACCACCCTTGCATGAACCACTTCGCCAAACTTTTGAAACTCTTCTCGCAGTCTCTCCGTTGTAGTACGTTTGCTAAGCCCAGACACGAAGAGGTTGGTGTTGGGATCCGCCATCTGTCGAGCGGAAGTAGCTCCGGAAGGTGGCGTTGAAGCGAAAGGGAAAGTGGGAGAAGACGGGGTTGGGTTCGAGCAGAAAAAGCGGCGCAAACCGCGGGGCGCCGCCGCCGCTGCAGCTCTCATCGTCAGAGCCATGGGTTTAAGCTTTGAAATGTAAAACCCTTGGTAGGTTGAAGGAGAGAGAGAAGAAAGACGAATAACNGAAACCGCCTATTGCTGATTTTTATATTTATATTAACAACAAAAACACTGGTGGGTGGGTACTGATCCACCGG
TARGET=719,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1384
PRIMER_LEFT_PENALTY=0.073989
PRIMER_RIGHT_PENALTY=0.064372
PRIMER_LEFT_SEQUENCE=TCGCAGTCTCTCCGTTGTAGTA
PRIMER_RIGHT_SEQUENCE=CGGTTTCNGTTATTCGTCTTTC
PRIMER_LEFT=470,22
PRIMER_RIGHT=750,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=4.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.2182
PRIMER_LEFT_1_PENALTY=0.153836
PRIMER_RIGHT_1_PENALTY=0.064372
PRIMER_LEFT_1_SEQUENCE=AGTACGTTTGCTAAGCCCAGAC
PRIMER_RIGHT_1_SEQUENCE=CGGTTTCNGTTATTCGTCTTTC
PRIMER_LEFT_1=488,22
PRIMER_RIGHT_1=750,22
PRIMER_LEFT_1_TM=59.846
PRIMER_RIGHT_1_TM=59.936
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.2273
PRIMER_LEFT_2_PENALTY=0.162964
PRIMER_RIGHT_2_PENALTY=0.064372
PRIMER_LEFT_2_SEQUENCE=TGTAGTACGTTTGCTAAGCCCA
PRIMER_RIGHT_2_SEQUENCE=CGGTTTCNGTTATTCGTCTTTC
PRIMER_LEFT_2=485,22
PRIMER_RIGHT_2=750,22
PRIMER_LEFT_2_TM=59.837
PRIMER_RIGHT_2_TM=59.936
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.2716
PRIMER_LEFT_3_PENALTY=0.073989
PRIMER_RIGHT_3_PENALTY=0.197640
PRIMER_LEFT_3_SEQUENCE=TCGCAGTCTCTCCGTTGTAGTA
PRIMER_RIGHT_3_SEQUENCE=CAATAGGCGGTTTCNGTTATTC
PRIMER_LEFT_3=470,22
PRIMER_RIGHT_3=757,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=59.802
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.50
PRIMER_LEFT_3_END_STABILITY=4.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.3515
PRIMER_LEFT_4_PENALTY=0.153836
PRIMER_RIGHT_4_PENALTY=0.197640
PRIMER_LEFT_4_SEQUENCE=AGTACGTTTGCTAAGCCCAGAC
PRIMER_RIGHT_4_SEQUENCE=CAATAGGCGGTTTCNGTTATTC
PRIMER_LEFT_4=488,22
PRIMER_RIGHT_4=757,22
PRIMER_LEFT_4_TM=59.846
PRIMER_RIGHT_4_TM=59.802
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.50
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_05061 Ps_contig_mira-and-tgicl-ass_franssen_36379
SEQUENCE=CCTTCAAAAGTAATTTTCTAGGAACAGTGGCGTRTGGCGTATTCATGTCCACTTTTTTCTGCAGGCCTCTGCCATTGTGAAGGAGACTTATCTTCCCGTAGTCCAGAAGGTGTTCTTGAATTCCCATCCCTTGGAACCCATCTCTTCTCTTCATACTTTGCACGAATGAAATTTTCAATTCCAACCCTGTCGTAGTTTGGAGGTAATTCTGCTTCCCAGAAACTATTGGCTTTATCGTTTCCCATTGAATGAATGAATGCAACTTGCTCTGGAAGCCAAGTGTCAAGAGTTGCAGAACGAACCTTTGATATATGTACTCCCAAACTTCTATGTATTCCTGAACATTGCATGCATATAAAGATGCCAAGATTTACACTGGCCCATCTCGGAGCTTTAGATTTGCAGTCAGCACATTCCCGATTCTCGGGTAATTTAAGCAGTCCTTCCAGTATCTTCTTGTGCTTGGCGTTAAGCTCTTTGGTAACGTTGGCCTTTCCGTTCATGGCGATTGGTTGAAGAAGAGCAACAAAGACGAATCGGAGAGGGAATTAGCAGAAAGCGAAAGCGAGAGAGAGGAGGAAGAAGAAGATCCAAGGTCAAATTGGTCTTTTTGTGCTCTTTCCCCTCTCTCTATCGAACTAAGACTCTNCGTTNACTNCCCGCGTACT
TARGET=246,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05062 Ps_contig_mira-and-tgicl-ass_franssen_36380
SEQUENCE=TATCGGTAGGTGNGATNTAAGTTNTACTAGACGAGCTNAAGTGCATCATCTCNATAAAGAAATCAAGATCAGCCATAACACCRGYATTATGCTGTGCAATCCAATTTCCCTTGGAAGAACTCCTCGATTGATTCAGAAACGACTCATTAATTGCAACAACGGTAGGAACAATAAAGCAAGGTTCAACATTACCAGCAAACCCCATCTTGGTATACCCGGAGCCATTGTCGATGACTACAGCAGGACGAGAAGCAGCTGGGTCCATTAGAAAAAGCTGAAAAGTCGCAAAATTTGAAAGTGGATCCGTAAGAGTGAAATTCAGATCCAGTAGCGAGATTTATACAGAAGATCATGATCGTGAAGAGAACAACAATGAAGAAGATGACGACGATGTTGGTGATGAAGACTTTGATCCCAAGTGAAAGAACCGAGTGGGAAGATAAAAGTGGTTGGTGATAGCTCTATCACTACCCATTTGTATTGAACTATAATAAAATTACCAAGATTACAAAACACGGTAGAGGGGTTACATAGGTCCCATTAGTTTGAGAAACGTTATGTTTTGTTTTTCCTAGAGATGATTTCTTCCTTCACAATCAAACAATGAATGAATGGACCCTTTTTTTCTTTCTCTATTCTCTCTGTTCTTCTTTCTTCTTCCATTCATTTTTAGTATGATGYTGCAATTTCAAGTCCTAGGATTATTGTAACATCTTGTGTAATACACTTGTATTATTGATATTTACAACAANNANATTTGTGAGTACCT
TARGET=603,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05063 Ps_contig_mira-and-tgicl-ass_franssen_36381
SEQUENCE=AACTAATAATTAAATGNNGGCGGATAAGAAGGGTAATACGGCTCTTCACATGGCTACAAGGAAAGGCCGATCACAGATAGTGAGCCTCTTGTTAAGCTATTCGGCTGTGGATGTTAATGCCATCAATAAGCAACAAGAAACAGCTTTGGATTTGGCAGATAAACTTCCATATGGATCTTCAGCTTTAGAAATCCAAGAAGCACTTTCAGAATATGGTGCTAAATATGCAAGACATGTTGGTAAATTAGATGAAGCAATGGAACTTAAAAGAACTGTAAGTGATATAAAGCATGAGGTGCAATCACAGCTTATTCAAAACGAAAAAACTCGCCGGCGAGTTTCTGGTATAGCTAAGGAACTGAAGAAACTTCATAGAGAAGCTGTTCAAAACACCATTAACTCTGTCACGCTCATTGCAGTCCTTTTCGCCTCAATAGCGTTCTTGGCCATATTCAATTTGCCAGGTCAATATATCATGGAAGGACCACAGACAGGAAAATCTAACATAGCCGATCATGTTGGATTCCAAGTTTTCTGTCTCTTGAATTCGACGTCTCTTTTCATATCTCTCGCTGTTGTTGTTGTTCAAATCACTTTGGTTGCTTGGGATACCAGGGCTCAGAAGCAAATTGTGTCGGTCGTTAACAAGTTAATGTGGGCTGCCTGTGCTTGCACGTGTGGCGCGTTCTTGGCTATAGCTTTCGAGGTTGTTGGAAAGAAAAAATGGATGGCCATCACCATAACTGCTGTAGGGACACCAATTCTAGTCGGGACTCTAGCGAGCATGTGCTACTTTGTTTTTCGACAACATTTTGGTATTTTTCAGAGCGATTCCCARAGACGAATCAAACGGGCAAGTGGAAGCAAATCTTTCTCGTGGTCTTACTCTGCAAATATATCGGATTTAGACGAATACAATTCAGACATTGAGAAAATCTATGCTCTGTAAGCTAGCGATCACAATCGTGTCTGTCTTACCACACACGATATACCGCTCTCGATATGTATAGTATAGTTCAAATAAGTTTTGGTCTACAAAGTACAGCTTCTAACAGTTATCAGTATAAGAATGATCAAGATATCAATGATTGTAATTTTATAATATATCAATGGTTGT
TARGET=574,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05064 Ps_contig_mira-and-tgicl-ass_franssen_36383
SEQUENCE=AGTTCTTCCCTTTTCGTTTAACCTAGCAAAATATACAGCAGCCTCTGTAATTCAAGCACGCCGGAAATTATATAGTAAACTGTGTTGATGATTTACCAATATGAATAAGTTATTGGAGTTTGGGAAGAAATTCATGTTTTATGCTAGGGTACTTTCTGGATATGAAGAGCGAAGAATCCGAAATTTTAGGCTGCAGCTTGAACAACGCGTTCAACAGGCACAAGCAAAGAAAGCAGCCATAAATAAAGTACCTGAGCAGATCATTTTATCTGAAGTTCGTCGAATGGTTGAAGAGATGCAAGCTTTGAACAAGAAGCTGGAAGAAACAGAGGTAGCCATTGAAGAATACTTCAAACCTCTTGACAAGGAGGCTGAGTTTTTAATGAAAATGCAACTACAAGGTGAAGAGAAAACATCGGAGATGATGATGAAAGCACTACAAGAACAAGCTATGCGTCGACAAGCTGAAGCAGAAAAAAGTACAAGTGTGCATCAAACAGAAAATGTTGAAACCAACCAGAAAGAACCCGAGACTCTGGTGACAACATGTATAGGAGAGGAAGAAAAAACCTTGAAGGACAATATGCATTAAGCAGACAAGGAAGAGAAAACTTTGAAGAACAAGCTATTTGTATTATTTAAAATTGCATCTTGAGGTGCGATCATGTTAACTTCAACATTTTTTTACGAGGTTTGATTTAGAACTCTTTGAGCCATTGGCTATTGGCTATGTAGAGGGTATGGATCTGATTGCTCCGGGCAGTAGCCTTCTGAGAAAGAGAATAAAACCAACCATTCCTCCATTTGAGCTGCAAAACAAATGGTGATTGAGAGAATATGCACATAAATGTGTTATTTGAGTATCAAAATATGACATTGTATGTGACGAGGATTATGTTTATCCTTAAATTTATTTATTTTTCATCTTCTACAACTTACATGTATTAAAGAAAAACACTTTGTA
TARGET=909,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05065 Ps_contig_mira-and-tgicl-ass_franssen_36386
SEQUENCE=AGTTRTTAAACAATTCGTTCATTAGACTCTAAAATCTACATATAAAAACTTCCATATTTTTCACCAACAAGTGCGGAGAGGAATAGGGTGAGTTACATTGAAAGGACTGATAAAAAACATTGCTACGCTGTCCAGTTCAAAACTTGATGGTCAATACTTTCATTTAACTGGGGTACACTATATATAATCAGCGTGCAGAAAATTACATTGGTGTTTGTCTTCAGTATGGAGTGTAGAGAAGTGTACAAGACAAGATCAGAAAGCTCTCATCCATCCACTCCAAACTGTATCAGGAAAAAATATTATGGGGAGGGAAATAATCGGTAAAATACATGAAACACTAGGTAAAGCCCCTCCCCAACACCTGAAGCTGTTGAGATATTCAACATGTGAAGCAAAGAATATATCATGGGGCTGCTCTCATCGAATCTTGCCTTCTAAGGGGAATCCCTTCAACGTCATCAAAGACATCTTCTTCTGAGTGCTTATTAAGTTTAATGATAGATGAACCATCAGTCACCATATCCTTAGTTCTGTGCAGAAGAAAAGTTCCTGAAAGTATTGTCACAAATCCACATATTTCTGTGATAACTTGAGTTGGACTTTGCCGATCCCAGTCCTTAAACATGATAACACTAGCCACAATGGTTAATGTTGTGAACATAACATAATAAATGGGAGATACCACTGCCGTATTGAAAGTATCCAGCGCCTTATTTAAATAATTCATTTGTGTAAGAATACAAACAGTTACAACTAACACGAATACCCAAGTTTGAGGGTAAATTAGCTGATTCATCCCAGACAGAGTTAACTTTATGGCAATTCCAAGAGCCTTAACACTCATAACCGATAGGGAACCCACAAGGGAACAGACACCAACATAAACCATTATGTGTGTCTGGCCATATAGAGGAATGAAGTGGAAGATAAGAATAAAAGTAGCTGTTATAACCAATGCTGCATAAAAGAGAAAGGCTGGATCCATAGCAAGACCCCACACTTCTGGCACTGATTCAATTACCCTTTCTTGAGGAGCATGTAGAACAATTGTTGTAGACCCCACAACACACAAAGCGCAACCAAGAACTCCAAAGATATGTAGTCTCTCCCGTAAGATAATATGGGCAAGAGCAGCACTGATAATAATGCTAAGAGCGCCAAGAGGAGTGACCAATATAGCAGGAGCAAATGCATATGCTGCAAAATTGGCAATCTCTCCAACAATCATTGTTATCATGCCCACCCACCAAAGTGGTTCATACAGGTATGAGTAGCCTCCACTTCCTGCCCTAACACCACTTGCACCGGCCTTTTTCAAACCTTTCTTTTTAACAATGAAACTAGCACCGATGAAGAAACTCGAAGAAAGAGCAAGAACTAAACCTTTAACGTTATCGGAGGACATTCCTTCGCGCCAGCTTGAACTCGTAGACAACGTCGACGCTGAAGAAGAAGAAGATGAAGTCGCCATCTCGCGAAAATACAAAAACCCTAGNGAGTGGGAGCGAGAT
TARGET=1449,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05066 Ps_contig_mira-and-tgicl-ass_franssen_36388
SEQUENCE=ACTCTCTTCATCATTCTGAATTTGAGTTAACCAGTGATGCTGAAATTCTTGACTACCACTGCTCTTGTTNGCAACAACAACAACAACACTATGTTCCTCCTACTCACAACACCATCTGCAAAACACTACTACCCATCCATTTTCAACTTCTTCTCCACTTCCACCACAAACCCACAATCTTTCACCGTCTCTTACCTCATCAACAATTGCGGTTTCTCCCTAGAAACCGCTTCCAAAGCTTCTCACACTCTCACTCTCTCCAACTCCCAAAACCCGATTCAATTCTCGCCTTCTTCACTTCCCACGGCTTCTCCGTCTCTCAACTTCGCGATATCATCAGAAGGGAGCCCTGGATACTCTCATGCGACCACACCAAGGTAGTTTTACCAAAGTTTCGATTTTTACTTTCCAAAGGTGCGTCTTCCTCTGACATTGTTCGTA
TARGET=72,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3798
PRIMER_LEFT_PENALTY=0.248900
PRIMER_RIGHT_PENALTY=0.130883
PRIMER_LEFT_SEQUENCE=CCAGTGATGCTGAAATTCTTGA
PRIMER_RIGHT_SEQUENCE=CGCAATTGTTGATGAGGTAAGA
PRIMER_LEFT=30,22
PRIMER_RIGHT=210,22
PRIMER_LEFT_TM=60.249
PRIMER_RIGHT_TM=60.131
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.3947
PRIMER_LEFT_1_PENALTY=0.248900
PRIMER_RIGHT_1_PENALTY=0.145769
PRIMER_LEFT_1_SEQUENCE=CCAGTGATGCTGAAATTCTTGA
PRIMER_RIGHT_1_SEQUENCE=GTTTTGGGAGTTGGAGAGAGTG
PRIMER_LEFT_1=30,22
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=60.249
PRIMER_RIGHT_1_TM=60.146
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.3947
PRIMER_LEFT_2_PENALTY=0.248900
PRIMER_RIGHT_2_PENALTY=0.145769
PRIMER_LEFT_2_SEQUENCE=CCAGTGATGCTGAAATTCTTGA
PRIMER_RIGHT_2_SEQUENCE=GGTGGAAGTGGAGAAGAAGTTG
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=164,22
PRIMER_LEFT_2_TM=60.249
PRIMER_RIGHT_2_TM=60.146
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=135
PRIMER_PAIR_PENALTY_3=0.4198
PRIMER_LEFT_3_PENALTY=0.248900
PRIMER_RIGHT_3_PENALTY=0.170871
PRIMER_LEFT_3_SEQUENCE=CCAGTGATGCTGAAATTCTTGA
PRIMER_RIGHT_3_SEQUENCE=TGAGAGTGTGAGAAGCTTTGGA
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=252,22
PRIMER_LEFT_3_TM=60.249
PRIMER_RIGHT_3_TM=60.171
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.4347
PRIMER_LEFT_4_PENALTY=0.248900
PRIMER_RIGHT_4_PENALTY=0.185822
PRIMER_LEFT_4_SEQUENCE=CCAGTGATGCTGAAATTCTTGA
PRIMER_RIGHT_4_SEQUENCE=GAAGAAGTTGAAAATGGATGGG
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=152,22
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=59.814
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_05067 Ps_contig_mira-and-tgicl-ass_franssen_36392
SEQUENCE=AGTTTTAAAAGGGAAAAGGGTTTTACGTNAATTTTCTAAAATTAAGTCACAAAACATCATCCTCATCATCATATGATAGAATATTATTCATTATTGAACAACCATGATATCTATACATCAAAAACATAACAATTACCCAACAGTCTTAAAAGACTTATGAACATGAGTAAGAACATCCTTATCCCTATTCCACTCAGGAGTAGGCGCATTAACAAAATGAGCATAAAGCTTGTTATTAGCACAAGTAACAGAAATCAAACTATGATAACCAACACCATCAATCTCATAAACATAATAAACTTGACCCTTTTCATCTCTCACTTCCTCAGAACTCACATTATCAGCAGTACCAATAATATCCTGCAAATTCACATCAGAAAGAGCCAAGTTGTTCAAAACAGCATCTTTTGGTGCTAACTGACGAAACCCAGAAAGGTAAGTTAAATACTCTTTCTCTGTTTTCTTCTTTGGGTTATAGAATTCACTGTCTGTTCCATTTGTTCCCTTTTCGACTTTCGAGATCAAGCGCTCCTTCCAGCCTTCTGGAACGTCGTAGATGTATTCGGCTGAGTCTTTCTTGTTTGAGTTGCCGCCGTATCCGCCGTAGTTTGAGGCGCTTGCGGCGGTTCCGTAGTAGAGATTGTAGGGAGGTGAAGGTGATGATTCTGCTGCTATGGAGGGTGGGGATGTTGTGAGAATGGTGGCTATGGCTGCTGCTGTGGCTGCTGTTAAGGTGGTGGATAGCAATGGGTGTTGGTGTTTGAAAGAGATGAGTGGTTTTGATATGGTGGTGGTTGTGGTGGTGGTGGTGGCGGTGGAGGAGGAGGAGAAAATGGGTGGTGGTGATGAGAAAATGGTGGCCATTGTTGTTTTTTGGATATGTTTGTTATGTTTTGTTGTCCATTGAAAGTTCCAAAAAATAATGTTTTCNTTTTTTTCTTATCTATT
TARGET=797,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0594
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=1.059316
PRIMER_LEFT_SEQUENCE=GATGTTGTGAGAATGGTGGCTA
PRIMER_RIGHT_SEQUENCE=CATTTTCTCCTCCTCCTCCAC
PRIMER_LEFT=685,22
PRIMER_RIGHT=834,21
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.059
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=150
PRIMER_PAIR_PENALTY_1=1.0772
PRIMER_LEFT_1_PENALTY=0.017846
PRIMER_RIGHT_1_PENALTY=1.059316
PRIMER_LEFT_1_SEQUENCE=TGGAACGTCGTAGATGTATTCG
PRIMER_RIGHT_1_SEQUENCE=CATTTTCTCCTCCTCCTCCAC
PRIMER_LEFT_1=543,22
PRIMER_RIGHT_1=834,21
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=60.059
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=1.1074
PRIMER_LEFT_2_PENALTY=0.048131
PRIMER_RIGHT_2_PENALTY=1.059316
PRIMER_LEFT_2_SEQUENCE=AACGTCGTAGATGTATTCGGCT
PRIMER_RIGHT_2_SEQUENCE=CATTTTCTCCTCCTCCTCCAC
PRIMER_LEFT_2=546,22
PRIMER_RIGHT_2=834,21
PRIMER_LEFT_2_TM=60.048
PRIMER_RIGHT_2_TM=60.059
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=1.1984
PRIMER_LEFT_3_PENALTY=0.139083
PRIMER_RIGHT_3_PENALTY=1.059316
PRIMER_LEFT_3_SEQUENCE=AGGTGAAGGTGATGATTCTGCT
PRIMER_RIGHT_3_SEQUENCE=CATTTTCTCCTCCTCCTCCAC
PRIMER_LEFT_3=648,22
PRIMER_RIGHT_3=834,21
PRIMER_LEFT_3_TM=60.139
PRIMER_RIGHT_3_TM=60.059
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=1.2252
PRIMER_LEFT_4_PENALTY=0.000037
PRIMER_RIGHT_4_PENALTY=1.225160
PRIMER_LEFT_4_SEQUENCE=GATGTTGTGAGAATGGTGGCTA
PRIMER_RIGHT_4_SEQUENCE=CATTTTCTCATCACCACCACC
PRIMER_LEFT_4=685,22
PRIMER_RIGHT_4=855,21
PRIMER_LEFT_4_TM=60.000
PRIMER_RIGHT_4_TM=60.225
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_05068 Ps_contig_mira-and-tgicl-ass_franssen_36392
SEQUENCE=AGTTTTAAAAGGGAAAAGGGTTTTACGTNAATTTTCTAAAATTAAGTCACAAAACATCATCCTCATCATCATATGATAGAATATTATTCATTATTGAACAACCATGATATCTATACATCAAAAACATAACAATTACCCAACAGTCTTAAAAGACTTATGAACATGAGTAAGAACATCCTTATCCCTATTCCACTCAGGAGTAGGCGCATTAACAAAATGAGCATAAAGCTTGTTATTAGCACAAGTAACAGAAATCAAACTATGATAACCAACACCATCAATCTCATAAACATAATAAACTTGACCCTTTTCATCTCTCACTTCCTCAGAACTCACATTATCAGCAGTACCAATAATATCCTGCAAATTCACATCAGAAAGAGCCAAGTTGTTCAAAACAGCATCTTTTGGTGCTAACTGACGAAACCCAGAAAGGTAAGTTAAATACTCTTTCTCTGTTTTCTTCTTTGGGTTATAGAATTCACTGTCTGTTCCATTTGTTCCCTTTTCGACTTTCGAGATCAAGCGCTCCTTCCAGCCTTCTGGAACGTCGTAGATGTATTCGGCTGAGTCTTTCTTGTTTGAGTTGCCGCCGTATCCGCCGTAGTTTGAGGCGCTTGCGGCGGTTCCGTAGTAGAGATTGTAGGGAGGTGAAGGTGATGATTCTGCTGCTATGGAGGGTGGGGATGTTGTGAGAATGGTGGCTATGGCTGCTGCTGTGGCTGCTGTTAAGGTGGTGGATAGCAATGGGTGTTGGTGTTTGAAAGAGATGAGTGGTTTTGATATGGTGGTGGTTGTGGTGGTGGTGGTGGCGGTGGAGGAGGAGGAGAAAATGGGTGGTGGTGATGAGAAAATGGTGGCCATTGTTGTTTTTTGGATATGTTTGTTATGTTTTGTTGTCCATTGAAAGTTCCAAAAAATAATGTTTTCNTTTTTTTCTTATCTATT
TARGET=817,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2252
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=1.225160
PRIMER_LEFT_SEQUENCE=GATGTTGTGAGAATGGTGGCTA
PRIMER_RIGHT_SEQUENCE=CATTTTCTCATCACCACCACC
PRIMER_LEFT=685,22
PRIMER_RIGHT=855,21
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=60.225
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=1.2813
PRIMER_LEFT_1_PENALTY=0.243148
PRIMER_RIGHT_1_PENALTY=1.038144
PRIMER_LEFT_1_SEQUENCE=GTGATGATTCTGCTGCTATGGA
PRIMER_RIGHT_1_SEQUENCE=ACCATTTTCTCATCACCACCAC
PRIMER_LEFT_1=656,22
PRIMER_RIGHT_1=857,22
PRIMER_LEFT_1_TM=60.243
PRIMER_RIGHT_1_TM=61.038
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=1.3586
PRIMER_LEFT_2_PENALTY=0.320415
PRIMER_RIGHT_2_PENALTY=1.038144
PRIMER_LEFT_2_SEQUENCE=GCGGTTCCGTAGTAGAGATTGT
PRIMER_RIGHT_2_SEQUENCE=ACCATTTTCTCATCACCACCAC
PRIMER_LEFT_2=622,22
PRIMER_RIGHT_2=857,22
PRIMER_LEFT_2_TM=59.680
PRIMER_RIGHT_2_TM=61.038
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=1.3642
PRIMER_LEFT_3_PENALTY=0.139083
PRIMER_RIGHT_3_PENALTY=1.225160
PRIMER_LEFT_3_SEQUENCE=AGGTGAAGGTGATGATTCTGCT
PRIMER_RIGHT_3_SEQUENCE=ACCATTTTCTCATCACCACCA
PRIMER_LEFT_3=648,22
PRIMER_RIGHT_3=857,21
PRIMER_LEFT_3_TM=60.139
PRIMER_RIGHT_3_TM=60.225
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=1.3642
PRIMER_LEFT_4_PENALTY=0.139083
PRIMER_RIGHT_4_PENALTY=1.225160
PRIMER_LEFT_4_SEQUENCE=AGGTGAAGGTGATGATTCTGCT
PRIMER_RIGHT_4_SEQUENCE=CCACCATTTTCTCATCACCAC
PRIMER_LEFT_4=648,22
PRIMER_RIGHT_4=859,21
PRIMER_LEFT_4_TM=60.139
PRIMER_RIGHT_4_TM=60.225
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_05069 Ps_contig_mira-and-tgicl-ass_franssen_36393
SEQUENCE=CTTTCTGCACCACTTTATACTAAGAGGTATGAGATTGTGAATGGTGTTATTGAGGTTGAAGGAATAACAGATGAAACAGTACCCGGAGAAGAAAACAAAGACAGCTGAAGAGAAAGGAGTGCCTAACTTTTGGCTAACTGCATTGAAGACTCATGAAACACTGGCCGGAGAGATTACTGAACGTGATGAGGAGGCCCTCAAGTATCTCAACGATATCAAGTGGTGTAAAATCGACAATCCTAAGGGTTTCAAGTTGGACTTCCATTTTGATTCTAATCCTTATTTTCGAAATTCTGTGCTGACAAAAACATATCACATGATTGAAGATGATGACCCTATATTAGAGAAATCAATTGGAACGGTAATTGAGTGGAATCTAGGAAAGTGTTTGACACAAAAGCTTCTGAAGAAGAAGCCAAAGAAAGGATCAAAAAATGAGAAACCAATCATAAAAACTAGAAAAATGTGAAAGTTTTTTCAACATTTTCAACCCTCCCCAAATTCCCGTGGATGATGATGAAATTGATGACGATGTTGTTGAAGAGCTTCAGAATTTGATGGAACATGATTATGACATTGGTTCTACAATACGAGACAAAATTATTCCTCATGCTGTGTCATGGTACACGGGAGAGGCTGTAGAGAGTGATTTTGAGGACACGGATGAAGATGGTGATGAAGATGAAGAGGATGATGAAGAGGATGAGGATGATGATGACGATGATGATGATGATGATGATGAGGAAGAAGATGAAGGGAAGGAGAAAAGCAAGAGCAAGACTTTTAGCACAGAGCACCCTCCTGATTGCAAGCAGCAGTAGTGTTTGATTGGGTGCAACAGTCCTGGATATATTTCCTGTCAGTGGTACAATTACAATTACTATAGAGTTTGGGTTATTTTAGTCAAAAGCTGCAATTTTACATTACTATTGTGACCTTTTCTTGCCTTTTTTTGGGTTAATCTTTTGGTCTCTGCTTAGGATTCTTGGGTTTCGATATTTATTTTACATTGCTATTGTCCGTCATTTTCTTGCCTTTTTTTGGGTTAATCTTTTGTTTTCTGCTTAGGATTATTGGGGTTCTTTGTGTTTGTTGTTTTGCCCATTGATTATTTTTTTATATTTCTCATTTTTCTTGGGTTTTAAAAGTAAGTGATGAGGATCTGTGTCATTGTCCATATTTACCTATTGTAAGAATCTGTACATATCTACTTCCATTACTATGATTATTCTATCAAACTTATTGAAAATTGG
TARGET=720,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0874
PRIMER_LEFT_PENALTY=0.036956
PRIMER_RIGHT_PENALTY=0.050443
PRIMER_LEFT_SEQUENCE=GAGGATGATGAAGAGGATGAGG
PRIMER_RIGHT_SEQUENCE=GAGGGTGCTCTGTGCTAAAAGT
PRIMER_LEFT=686,22
PRIMER_RIGHT=800,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.950
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=0.0874
PRIMER_LEFT_1_PENALTY=0.036956
PRIMER_RIGHT_1_PENALTY=0.050443
PRIMER_LEFT_1_SEQUENCE=AGGATGATGAAGAGGATGAGGA
PRIMER_RIGHT_1_SEQUENCE=GAGGGTGCTCTGTGCTAAAAGT
PRIMER_LEFT_1=687,22
PRIMER_RIGHT_1=800,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.950
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=0.0874
PRIMER_LEFT_2_PENALTY=0.036956
PRIMER_RIGHT_2_PENALTY=0.050443
PRIMER_LEFT_2_SEQUENCE=GAGGATGATGAAGAGGATGAGG
PRIMER_RIGHT_2_SEQUENCE=AGGGTGCTCTGTGCTAAAAGTC
PRIMER_LEFT_2=686,22
PRIMER_RIGHT_2=799,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=59.950
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=0.0874
PRIMER_LEFT_3_PENALTY=0.036956
PRIMER_RIGHT_3_PENALTY=0.050443
PRIMER_LEFT_3_SEQUENCE=AGGATGATGAAGAGGATGAGGA
PRIMER_RIGHT_3_SEQUENCE=AGGGTGCTCTGTGCTAAAAGTC
PRIMER_LEFT_3=687,22
PRIMER_RIGHT_3=799,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=59.950
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=0.0874
PRIMER_LEFT_4_PENALTY=0.036956
PRIMER_RIGHT_4_PENALTY=0.050443
PRIMER_LEFT_4_SEQUENCE=GAGGATGATGAAGAGGATGAGG
PRIMER_RIGHT_4_SEQUENCE=GGGTGCTCTGTGCTAAAAGTCT
PRIMER_LEFT_4=686,22
PRIMER_RIGHT_4=798,22
PRIMER_LEFT_4_TM=60.037
PRIMER_RIGHT_4_TM=59.950
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=113
=
PRIMER_SEQUENCE_ID=SSR_05070 Ps_contig_mira-and-tgicl-ass_franssen_36396
SEQUENCE=CAATTGCATTCAACCCTTCCATTGTTTACTAGAAACACTTCAAAATTCAAGCTTCTCACTGCCTTATCACCTTCTCCATTAACCGATTCTAGTACTAACCTTCAAGTGGATGATGATGATGATGAGGTAGAAGGTGAAACAGGTTCGGAGAAATTTGATTGGTACTCACAGTGGTACCCTTTGATGCCAATCTGTGATCTTGACAAAAGGGCACCTCATGGGAAGAAGGTTATGGGGATTGATGTGGTTGTGTGGTGGGATAGGAATGAAGGTGCATGGCAAGTGTTTGATGATGCGTGTCCTCATAGATTGGCACCTTTGTCTGAAGGAAGGATTGATCAATGGGGTAGGTTGCAGTGTGTGTATCATGGTTGGTGTTTTAATGGATCCGGTGATTGCAAGTTCATTCCTCAGGCACCTTCTGATGGACCTCCGATTCATACATCCAAGAAAGCATGTGTAGCTGCTTACCCAAGCATTGTGCAGAATGATATCTTGTGGTTTTGGCCAAATACTGATCCTCAATACAAAGATATTATTACAAGAAAAAAGCCACCATTCATACCAGAAATCGATGATCCGTCCTTTACTAGCTTAATGGGAAACCGAGAGATTGCTTACGGGTATGAAGTTTTGATTGAAAACCTCATGGACCCTTCGCATGTTCCATATGCACATTATGGATTAATGAAAACTCCACAACCAAAAGTGAAAGCTGATAGAGAAGGAGGAAGACCACTTGAATTGTCAATTGAAAAGTTAGATATCAATGGTTTCACTGCAAATCAGGGTTGGAGCAAAAGTAAATTTATGCCACCAAGCATCTTTTATGCATATAGTGATCCAAATAAACCCGCATCTTCTGATGAATCTAAGAAATCATCGGTTCAGAGGAAATTTTCTTTGATCTTTATTTGTGTTCCTGTTAGTCCCGGTAATAGCAGACTGATTTGGTGCTTCCCAAGAAACTTTGGACTGTGGATTGACAAAATTGTACCGCGATGGATGTTTCATGTTGGACAAAACTTGATTCTAGATTCAGATTTATATCTTCTGCATCTTGAGGAAAAGAAAATAATGGATGTTGGTCAAGCAAATTGGCATAAGGCCTGTTTTGTGCCAACAAAAGCAGATGCTCTTGTGATTGGTTTTAGAAAGTGGTTAAAGAAATACGCAGGCGGTGAAGTTGATTGGAGAGGAAAATATAGCGGTGCTCTTCCACCTACGCCTCCTAAAGAACAGCTTATGGACAGGTACTGGAGCCATGTGGTGAATTGCAAGAGTTGCAATTTGGCGTATAAGAGCCTCAATGTGGTGGAAGTGATGATGCAGATCATATCTGTTGCTTCAATTGGGATTGTTGCCATAATGAAGCAGGGTATGGTTTCGGCTGCAACAAGAAATTCAATGGTTGTGCTGGCAGTACTTTCATTCGCGCTGTCGCGGTTGTTAGCTCACTTCATATACAAAAACTTCCGTTATCATGACTACAACCACGCCTTTCGCTGAAATGTGTTTCCTTAGCTATTGGTGTGCATCTTGGTTATATATATATAGAGTGAAGTTCTACAAAAACAAAGCCAGTAACTGTTAGTACCTAAGAGGAGTTTGGAGCATCTGTATATGTATGTGATACATATTAAGTTCATAACAGGCACATCGATTGAAGATGCATACATGTTTGATTCCACTCTTGTGTGTGGTAATACTGTGAAGAGAAATGATATATGTACACTTCAAAAATTGAACACTCCAAAGAACATTGTTATTGATATTTAAAATTATAATTACATAGATATTGAGTTCTCTCCTTGT
TARGET=109,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1348
PRIMER_LEFT_PENALTY=0.101680
PRIMER_RIGHT_PENALTY=0.033137
PRIMER_LEFT_SEQUENCE=AAAATTCAAGCTTCTCACTGCC
PRIMER_RIGHT_SEQUENCE=CGAACCTGTTTCACCTTCTACC
PRIMER_LEFT=41,22
PRIMER_RIGHT=146,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=0.1348
PRIMER_LEFT_1_PENALTY=0.101680
PRIMER_RIGHT_1_PENALTY=0.033137
PRIMER_LEFT_1_SEQUENCE=AAAATTCAAGCTTCTCACTGCC
PRIMER_RIGHT_1_SEQUENCE=CCGAACCTGTTTCACCTTCTAC
PRIMER_LEFT_1=41,22
PRIMER_RIGHT_1=147,22
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=60.033
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=107
PRIMER_PAIR_PENALTY_2=0.1892
PRIMER_LEFT_2_PENALTY=0.086698
PRIMER_RIGHT_2_PENALTY=0.102490
PRIMER_LEFT_2_SEQUENCE=AGCTTCTCACTGCCTTATCACC
PRIMER_RIGHT_2_SEQUENCE=CAATCCTTCCTTCAGACAAAGG
PRIMER_LEFT_2=49,22
PRIMER_RIGHT_2=336,22
PRIMER_LEFT_2_TM=59.913
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.1892
PRIMER_LEFT_3_PENALTY=0.086698
PRIMER_RIGHT_3_PENALTY=0.102490
PRIMER_LEFT_3_SEQUENCE=GCTTCTCACTGCCTTATCACCT
PRIMER_RIGHT_3_SEQUENCE=CAATCCTTCCTTCAGACAAAGG
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=336,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=0.2042
PRIMER_LEFT_4_PENALTY=0.101680
PRIMER_RIGHT_4_PENALTY=0.102490
PRIMER_LEFT_4_SEQUENCE=AAAATTCAAGCTTCTCACTGCC
PRIMER_RIGHT_4_SEQUENCE=CAATCCTTCCTTCAGACAAAGG
PRIMER_LEFT_4=41,22
PRIMER_RIGHT_4=336,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_05071 Ps_contig_mira-and-tgicl-ass_franssen_36396
SEQUENCE=CAATTGCATTCAACCCTTCCATTGTTTACTAGAAACACTTCAAAATTCAAGCTTCTCACTGCCTTATCACCTTCTCCATTAACCGATTCTAGTACTAACCTTCAAGTGGATGATGATGATGATGAGGTAGAAGGTGAAACAGGTTCGGAGAAATTTGATTGGTACTCACAGTGGTACCCTTTGATGCCAATCTGTGATCTTGACAAAAGGGCACCTCATGGGAAGAAGGTTATGGGGATTGATGTGGTTGTGTGGTGGGATAGGAATGAAGGTGCATGGCAAGTGTTTGATGATGCGTGTCCTCATAGATTGGCACCTTTGTCTGAAGGAAGGATTGATCAATGGGGTAGGTTGCAGTGTGTGTATCATGGTTGGTGTTTTAATGGATCCGGTGATTGCAAGTTCATTCCTCAGGCACCTTCTGATGGACCTCCGATTCATACATCCAAGAAAGCATGTGTAGCTGCTTACCCAAGCATTGTGCAGAATGATATCTTGTGGTTTTGGCCAAATACTGATCCTCAATACAAAGATATTATTACAAGAAAAAAGCCACCATTCATACCAGAAATCGATGATCCGTCCTTTACTAGCTTAATGGGAAACCGAGAGATTGCTTACGGGTATGAAGTTTTGATTGAAAACCTCATGGACCCTTCGCATGTTCCATATGCACATTATGGATTAATGAAAACTCCACAACCAAAAGTGAAAGCTGATAGAGAAGGAGGAAGACCACTTGAATTGTCAATTGAAAAGTTAGATATCAATGGTTTCACTGCAAATCAGGGTTGGAGCAAAAGTAAATTTATGCCACCAAGCATCTTTTATGCATATAGTGATCCAAATAAACCCGCATCTTCTGATGAATCTAAGAAATCATCGGTTCAGAGGAAATTTTCTTTGATCTTTATTTGTGTTCCTGTTAGTCCCGGTAATAGCAGACTGATTTGGTGCTTCCCAAGAAACTTTGGACTGTGGATTGACAAAATTGTACCGCGATGGATGTTTCATGTTGGACAAAACTTGATTCTAGATTCAGATTTATATCTTCTGCATCTTGAGGAAAAGAAAATAATGGATGTTGGTCAAGCAAATTGGCATAAGGCCTGTTTTGTGCCAACAAAAGCAGATGCTCTTGTGATTGGTTTTAGAAAGTGGTTAAAGAAATACGCAGGCGGTGAAGTTGATTGGAGAGGAAAATATAGCGGTGCTCTTCCACCTACGCCTCCTAAAGAACAGCTTATGGACAGGTACTGGAGCCATGTGGTGAATTGCAAGAGTTGCAATTTGGCGTATAAGAGCCTCAATGTGGTGGAAGTGATGATGCAGATCATATCTGTTGCTTCAATTGGGATTGTTGCCATAATGAAGCAGGGTATGGTTTCGGCTGCAACAAGAAATTCAATGGTTGTGCTGGCAGTACTTTCATTCGCGCTGTCGCGGTTGTTAGCTCACTTCATATACAAAAACTTCCGTTATCATGACTACAACCACGCCTTTCGCTGAAATGTGTTTCCTTAGCTATTGGTGTGCATCTTGGTTATATATATATAGAGTGAAGTTCTACAAAAACAAAGCCAGTAACTGTTAGTACCTAAGAGGAGTTTGGAGCATCTGTATATGTATGTGATACATATTAAGTTCATAACAGGCACATCGATTGAAGATGCATACATGTTTGATTCCACTCTTGTGTGTGGTAATACTGTGAAGAGAAATGATATATGTACACTTCAAAAATTGAACACTCCAAAGAACATTGTTATTGATATTTAAAATTATAATTACATAGATATTGAGTTCTCTCCTTGT
TARGET=1545,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4582
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.362762
PRIMER_LEFT_SEQUENCE=CCATAATGAAGCAGGGTATGGT
PRIMER_RIGHT_SEQUENCE=TCGATGTGCCTGTTATGAACTT
PRIMER_LEFT=1363,22
PRIMER_RIGHT=1662,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.637
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.4737
PRIMER_LEFT_1_PENALTY=0.110942
PRIMER_RIGHT_1_PENALTY=0.362762
PRIMER_LEFT_1_SEQUENCE=TTATCATGACTACAACCACGCC
PRIMER_RIGHT_1_SEQUENCE=TCGATGTGCCTGTTATGAACTT
PRIMER_LEFT_1=1478,22
PRIMER_RIGHT_1=1662,22
PRIMER_LEFT_1_TM=59.889
PRIMER_RIGHT_1_TM=59.637
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.5147
PRIMER_LEFT_2_PENALTY=0.151911
PRIMER_RIGHT_2_PENALTY=0.362762
PRIMER_LEFT_2_SEQUENCE=TAGCTATTGGTGTGCATCTTGG
PRIMER_RIGHT_2_SEQUENCE=TCGATGTGCCTGTTATGAACTT
PRIMER_LEFT_2=1521,22
PRIMER_RIGHT_2=1662,22
PRIMER_LEFT_2_TM=60.152
PRIMER_RIGHT_2_TM=59.637
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.5501
PRIMER_LEFT_3_PENALTY=0.187326
PRIMER_RIGHT_3_PENALTY=0.362762
PRIMER_LEFT_3_SEQUENCE=TTGTGCTGGCAGTACTTTCATT
PRIMER_RIGHT_3_SEQUENCE=TCGATGTGCCTGTTATGAACTT
PRIMER_LEFT_3=1411,22
PRIMER_RIGHT_3=1662,22
PRIMER_LEFT_3_TM=59.813
PRIMER_RIGHT_3_TM=59.637
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.5773
PRIMER_LEFT_4_PENALTY=0.110942
PRIMER_RIGHT_4_PENALTY=0.466342
PRIMER_LEFT_4_SEQUENCE=TTATCATGACTACAACCACGCC
PRIMER_RIGHT_4_SEQUENCE=ACCACACACAAGAGTGGAATCA
PRIMER_LEFT_4=1478,22
PRIMER_RIGHT_4=1703,22
PRIMER_LEFT_4_TM=59.889
PRIMER_RIGHT_4_TM=60.466
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=11.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_05072 Ps_contig_mira-and-tgicl-ass_franssen_36397
SEQUENCE=AAACAGGAGGCCTGCTGAATCATTGAAAACAAATGAACAGTACTACTGAAAGAAAGAAAGGCCTTGGTGATTGGATGAATATGATTAAACCTGCCAATGAAGAGAAAGATCATTGGGTCCCAGATGAAGCAGTCACAAAATGCACCGCATGTGGCACTGATTTTGGGGCTTTTAATCGCAAGCATCACTGTAGGAACTGTGGTGATATTTTCTGTGACAAATGTACGCATGGTAGAATTGCTCTAACTGCTGAAGAGAATGCTCAGCCAGTACGGGTTTGCGACAGGTGCATGGCAGAAGTGACTCAGAGGCTGATCAGTGCCAAGGAATCATCAAGTAAACCTCTTTTGCAGAGTCATGAGGATCTTGCTCGGAAACTTCAGGAGGAGCTGGAGAGAAATCGGAAGACATCAGGATCAGGTTCTAAGTCTGACGGAACTGGAAGACGGATGAAGGAAGTTGCATGTCCTATTTGCACTGTCCATCTGCAGGTTCAGGTACCTACCTCAGGTTCAGAGACCATCGAGTGTGGAGTTTGCCAGCATCCATTTCTTGTGAGTTCTTATTGAGAGTTCAGATAACATGGATACGGAAGGCGTCTTTGATGAAGTTCAAAAATCATTTACTTTACCGATCTATTGCAGCTTGCACATTAATGATATACCGATTGGGATTGCCTTATGTTTACTTTTTATCTTTGGGCTTCTTTTGTGACCATTAGTTGCTTGCATCAATTTATTTTTATCGTGTGAGCTTTGTTTTTATTTTATTATTATTATTATTATTATTATTATTCTTAAAATTTTGAACTTTTCATCTTGTGTCCTGTATAAAAAGCTGAACTTTGATGTATATGTGAAATTCATCCCGGTTTGAGTCCAATATGATGAATTGTTTTCTT
TARGET=48,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3525
PRIMER_LEFT_PENALTY=1.295085
PRIMER_RIGHT_PENALTY=0.057411
PRIMER_LEFT_SEQUENCE=GCCTGCTGAATCATTGAAAAC
PRIMER_RIGHT_SEQUENCE=CAGTTCCTACAGTGATGCTTGC
PRIMER_LEFT=9,21
PRIMER_RIGHT=198,22
PRIMER_LEFT_TM=59.705
PRIMER_RIGHT_TM=59.943
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=1.3845
PRIMER_LEFT_1_PENALTY=1.295085
PRIMER_RIGHT_1_PENALTY=0.089381
PRIMER_LEFT_1_SEQUENCE=GCCTGCTGAATCATTGAAAAC
PRIMER_RIGHT_1_SEQUENCE=CAGTGATGCTTGCGATTAAAAG
PRIMER_LEFT_1=9,21
PRIMER_RIGHT_1=189,22
PRIMER_LEFT_1_TM=59.705
PRIMER_RIGHT_1_TM=59.911
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=1.4491
PRIMER_LEFT_2_PENALTY=1.295085
PRIMER_RIGHT_2_PENALTY=0.154023
PRIMER_LEFT_2_SEQUENCE=GCCTGCTGAATCATTGAAAAC
PRIMER_RIGHT_2_SEQUENCE=TACTGGCTGAGCATTCTCTTCA
PRIMER_LEFT_2=9,21
PRIMER_RIGHT_2=271,22
PRIMER_LEFT_2_TM=59.705
PRIMER_RIGHT_2_TM=60.154
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=1.5366
PRIMER_LEFT_3_PENALTY=1.295085
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=GCCTGCTGAATCATTGAAAAC
PRIMER_RIGHT_3_SEQUENCE=TCTTTCTCTTCATTGGCAGGTT
PRIMER_LEFT_3=9,21
PRIMER_RIGHT_3=108,22
PRIMER_LEFT_3_TM=59.705
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=1.5800
PRIMER_LEFT_4_PENALTY=1.295085
PRIMER_RIGHT_4_PENALTY=0.284927
PRIMER_LEFT_4_SEQUENCE=GCCTGCTGAATCATTGAAAAC
PRIMER_RIGHT_4_SEQUENCE=CCTACAGTGATGCTTGCGATTA
PRIMER_LEFT_4=9,21
PRIMER_RIGHT_4=193,22
PRIMER_LEFT_4_TM=59.705
PRIMER_RIGHT_4_TM=60.285
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_05073 Ps_contig_mira-and-tgicl-ass_franssen_36397
SEQUENCE=AAACAGGAGGCCTGCTGAATCATTGAAAACAAATGAACAGTACTACTGAAAGAAAGAAAGGCCTTGGTGATTGGATGAATATGATTAAACCTGCCAATGAAGAGAAAGATCATTGGGTCCCAGATGAAGCAGTCACAAAATGCACCGCATGTGGCACTGATTTTGGGGCTTTTAATCGCAAGCATCACTGTAGGAACTGTGGTGATATTTTCTGTGACAAATGTACGCATGGTAGAATTGCTCTAACTGCTGAAGAGAATGCTCAGCCAGTACGGGTTTGCGACAGGTGCATGGCAGAAGTGACTCAGAGGCTGATCAGTGCCAAGGAATCATCAAGTAAACCTCTTTTGCAGAGTCATGAGGATCTTGCTCGGAAACTTCAGGAGGAGCTGGAGAGAAATCGGAAGACATCAGGATCAGGTTCTAAGTCTGACGGAACTGGAAGACGGATGAAGGAAGTTGCATGTCCTATTTGCACTGTCCATCTGCAGGTTCAGGTACCTACCTCAGGTTCAGAGACCATCGAGTGTGGAGTTTGCCAGCATCCATTTCTTGTGAGTTCTTATTGAGAGTTCAGATAACATGGATACGGAAGGCGTCTTTGATGAAGTTCAAAAATCATTTACTTTACCGATCTATTGCAGCTTGCACATTAATGATATACCGATTGGGATTGCCTTATGTTTACTTTTTATCTTTGGGCTTCTTTTGTGACCATTAGTTGCTTGCATCAATTTATTTTTATCGTGTGAGCTTTGTTTTTATTTTATTATTATTATTATTATTATTATTATTCTTAAAATTTTGAACTTTTCATCTTGTGTCCTGTATAAAAAGCTGAACTTTGATGTATATGTGAAATTCATCCCGGTTTGAGTCCAATATGATGAATTGTTTTCTT
TARGET=767,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3498
PRIMER_LEFT_PENALTY=0.172839
PRIMER_RIGHT_PENALTY=0.177008
PRIMER_LEFT_SEQUENCE=TTTGTGACCATTAGTTGCTTGC
PRIMER_RIGHT_SEQUENCE=TCATCATATTGGACTCAAACCG
PRIMER_LEFT=707,22
PRIMER_RIGHT=889,22
PRIMER_LEFT_TM=60.173
PRIMER_RIGHT_TM=59.823
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.5404
PRIMER_LEFT_1_PENALTY=0.363436
PRIMER_RIGHT_1_PENALTY=0.177008
PRIMER_LEFT_1_SEQUENCE=ATCTTTGGGCTTCTTTTGTGAC
PRIMER_RIGHT_1_SEQUENCE=TCATCATATTGGACTCAAACCG
PRIMER_LEFT_1=693,22
PRIMER_RIGHT_1=889,22
PRIMER_LEFT_1_TM=59.637
PRIMER_RIGHT_1_TM=59.823
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=197
PRIMER_PAIR_PENALTY_2=0.6909
PRIMER_LEFT_2_PENALTY=0.513934
PRIMER_RIGHT_2_PENALTY=0.177008
PRIMER_LEFT_2_SEQUENCE=TATACCGATTGGGATTGCCTTA
PRIMER_RIGHT_2_SEQUENCE=TCATCATATTGGACTCAAACCG
PRIMER_LEFT_2=659,22
PRIMER_RIGHT_2=889,22
PRIMER_LEFT_2_TM=60.514
PRIMER_RIGHT_2_TM=59.823
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.7407
PRIMER_LEFT_3_PENALTY=0.563643
PRIMER_RIGHT_3_PENALTY=0.177008
PRIMER_LEFT_3_SEQUENCE=TGTGACCATTAGTTGCTTGCAT
PRIMER_RIGHT_3_SEQUENCE=TCATCATATTGGACTCAAACCG
PRIMER_LEFT_3=709,22
PRIMER_RIGHT_3=889,22
PRIMER_LEFT_3_TM=60.564
PRIMER_RIGHT_3_TM=59.823
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.7703
PRIMER_LEFT_4_PENALTY=0.593278
PRIMER_RIGHT_4_PENALTY=0.177008
PRIMER_LEFT_4_SEQUENCE=TTTACCGATCTATTGCAGCTTG
PRIMER_RIGHT_4_SEQUENCE=TCATCATATTGGACTCAAACCG
PRIMER_LEFT_4=626,22
PRIMER_RIGHT_4=889,22
PRIMER_LEFT_4_TM=59.407
PRIMER_RIGHT_4_TM=59.823
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_05074 Ps_contig_mira-and-tgicl-ass_franssen_36402
SEQUENCE=ATTGTCAACAACTCACCTTCTATCTCCTTGATTCCGTCCANTCTTCCTTCCTTGCTTCATTCCATGCTTCCACGCTTCATCATCATTCTGCACTTTCGCACTTCCTCATTTATCTCACTCTGCAGACTCTCTTTTCAGGATTCTGCAACTCTTTCTTCATCAATCTGCATCATTTTGCAGTAATCTCCATCCTCTGCAACTCCATTACTCTTCACGCAACCAAAATGCACAACACATATTGAAGAAGAAGAAGAGAAGCCTTGAAGAACTGGAATAGATCGAGAAGCTCGTACCTTTGAAGTTGGTATTGATTTGTCGAGTTACGGCGATCCCGGCACGGATCGATTTAAGAAGCGATTACCATCCGCATCGATTGGCAAACGTGAAATCAAGGACCTCTATTGCTCTCCAGTACGTATCGATAAGCACTTCTCCTTTTCAATTTATCTGTTTTTTCTCGCTTCCACTTGTATCGAACCCGAGGATGATAAAGATTGGAAGCCGATTTGCGACGTACGCGGCGTTCCTCCGATGCCAACACGATGATGCCGGTATTTCCAAATGCGATACCATGGTGATTGATGCTTGGAGCTTTTTGTTTAAGCGCTTTTGTCTTGATACATAAGCGTGTTTCCTTGCCGTTTATCCTCTTCAATCTCCACTCCTCAATATACCTCTTAAATTTCTGTGTCGACTCAAAGGAATTGATTTCAATCAATCAATCACAGTAAACCACCTTCTTCAACCGGTATTGCACCACACCAGTTCTGCATCAACTCTGCGATCTGCAACGATAATACGCAGATACACATCACATACACCGACTCGCAAACATACGCAGAAAATGCAACAAACTTACGTAGCTCCCCGCCGCGTACGATTTATCATAGGGAAAGTATTCAGAATTATCAAGAAGAATTCATACCCGCTCCCAAACCAG
TARGET=241,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3171
PRIMER_LEFT_PENALTY=0.165541
PRIMER_RIGHT_PENALTY=0.151533
PRIMER_LEFT_SEQUENCE=CATTACTCTTCACGCAACCAAA
PRIMER_RIGHT_SEQUENCE=GGAGAGCAATAGAGGTCCTTGA
PRIMER_LEFT=202,22
PRIMER_RIGHT=409,22
PRIMER_LEFT_TM=60.166
PRIMER_RIGHT_TM=59.848
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.3171
PRIMER_LEFT_1_PENALTY=0.165541
PRIMER_RIGHT_1_PENALTY=0.151533
PRIMER_LEFT_1_SEQUENCE=TACTCTTCACGCAACCAAAATG
PRIMER_RIGHT_1_SEQUENCE=GGAGAGCAATAGAGGTCCTTGA
PRIMER_LEFT_1=205,22
PRIMER_RIGHT_1=409,22
PRIMER_LEFT_1_TM=60.166
PRIMER_RIGHT_1_TM=59.848
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.3765
PRIMER_LEFT_2_PENALTY=0.016221
PRIMER_RIGHT_2_PENALTY=0.360320
PRIMER_LEFT_2_SEQUENCE=CTATCTCCTTGATTCCGTCCAN
PRIMER_RIGHT_2_SEQUENCE=ACGAGCTTCTCGATCTATTCCA
PRIMER_LEFT_2=19,22
PRIMER_RIGHT_2=290,22
PRIMER_LEFT_2_TM=59.984
PRIMER_RIGHT_2_TM=60.360
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1750
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.4088
PRIMER_LEFT_3_PENALTY=0.016221
PRIMER_RIGHT_3_PENALTY=0.392624
PRIMER_LEFT_3_SEQUENCE=CTATCTCCTTGATTCCGTCCAN
PRIMER_RIGHT_3_SEQUENCE=TCAAAGGTACGAGCTTCTCGAT
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=298,22
PRIMER_LEFT_3_TM=59.984
PRIMER_RIGHT_3_TM=60.393
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1750
PRIMER_RIGHT_3_END_STABILITY=8.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.4379
PRIMER_LEFT_4_PENALTY=0.165541
PRIMER_RIGHT_4_PENALTY=0.272373
PRIMER_LEFT_4_SEQUENCE=CATTACTCTTCACGCAACCAAA
PRIMER_RIGHT_4_SEQUENCE=TTCCAATCTTTATCATCCTCGG
PRIMER_LEFT_4=202,22
PRIMER_RIGHT_4=499,22
PRIMER_LEFT_4_TM=60.166
PRIMER_RIGHT_4_TM=60.272
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_05075 Ps_contig_mira-and-tgicl-ass_franssen_36402
SEQUENCE=ATTGTCAACAACTCACCTTCTATCTCCTTGATTCCGTCCANTCTTCCTTCCTTGCTTCATTCCATGCTTCCACGCTTCATCATCATTCTGCACTTTCGCACTTCCTCATTTATCTCACTCTGCAGACTCTCTTTTCAGGATTCTGCAACTCTTTCTTCATCAATCTGCATCATTTTGCAGTAATCTCCATCCTCTGCAACTCCATTACTCTTCACGCAACCAAAATGCACAACACATATTGAAGAAGAAGAAGAGAAGCCTTGAAGAACTGGAATAGATCGAGAAGCTCGTACCTTTGAAGTTGGTATTGATTTGTCGAGTTACGGCGATCCCGGCACGGATCGATTTAAGAAGCGATTACCATCCGCATCGATTGGCAAACGTGAAATCAAGGACCTCTATTGCTCTCCAGTACGTATCGATAAGCACTTCTCCTTTTCAATTTATCTGTTTTTTCTCGCTTCCACTTGTATCGAACCCGAGGATGATAAAGATTGGAAGCCGATTTGCGACGTACGCGGCGTTCCTCCGATGCCAACACGATGATGCCGGTATTTCCAAATGCGATACCATGGTGATTGATGCTTGGAGCTTTTTGTTTAAGCGCTTTTGTCTTGATACATAAGCGTGTTTCCTTGCCGTTTATCCTCTTCAATCTCCACTCCTCAATATACCTCTTAAATTTCTGTGTCGACTCAAAGGAATTGATTTCAATCAATCAATCACAGTAAACCACCTTCTTCAACCGGTATTGCACCACACCAGTTCTGCATCAACTCTGCGATCTGCAACGATAATACGCAGATACACATCACATACACCGACTCGCAAACATACGCAGAAAATGCAACAAACTTACGTAGCTCCCCGCCGCGTACGATTTATCATAGGGAAAGTATTCAGAATTATCAAGAAGAATTCATACCCGCTCCCAAACCAG
TARGET=711,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1507
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.055230
PRIMER_LEFT_SEQUENCE=TGCCGTTTATCCTCTTCAATCT
PRIMER_RIGHT_SEQUENCE=GGGGAGCTACGTAAGTTTGTTG
PRIMER_LEFT=636,22
PRIMER_RIGHT=868,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=60.055
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.1909
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.095445
PRIMER_LEFT_1_SEQUENCE=TGCCGTTTATCCTCTTCAATCT
PRIMER_RIGHT_1_SEQUENCE=GAGCGGGTATGAATTCTTCTTG
PRIMER_LEFT_1=636,22
PRIMER_RIGHT_1=930,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=60.095
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=0.1909
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.095445
PRIMER_LEFT_2_SEQUENCE=TGCCGTTTATCCTCTTCAATCT
PRIMER_RIGHT_2_SEQUENCE=AGCGGGTATGAATTCTTCTTGA
PRIMER_LEFT_2=636,22
PRIMER_RIGHT_2=929,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=294
PRIMER_PAIR_PENALTY_3=0.2379
PRIMER_LEFT_3_PENALTY=0.182682
PRIMER_RIGHT_3_PENALTY=0.055230
PRIMER_LEFT_3_SEQUENCE=TCCTCTTCAATCTCCACTCCTC
PRIMER_RIGHT_3_SEQUENCE=GGGGAGCTACGTAAGTTTGTTG
PRIMER_LEFT_3=645,22
PRIMER_RIGHT_3=868,22
PRIMER_LEFT_3_TM=59.817
PRIMER_RIGHT_3_TM=60.055
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.2781
PRIMER_LEFT_4_PENALTY=0.182682
PRIMER_RIGHT_4_PENALTY=0.095445
PRIMER_LEFT_4_SEQUENCE=TCCTCTTCAATCTCCACTCCTC
PRIMER_RIGHT_4_SEQUENCE=GAGCGGGTATGAATTCTTCTTG
PRIMER_LEFT_4=645,22
PRIMER_RIGHT_4=930,22
PRIMER_LEFT_4_TM=59.817
PRIMER_RIGHT_4_TM=60.095
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_05076 Ps_contig_mira-and-tgicl-ass_franssen_36403
SEQUENCE=ATAATTGTTTATATTTTTTTGGCCAAGTGAGCCGAAAAAATAAAGGATCAATCAGCCAAGGGAGTGGGGGTAAGGTTGCTCCTCTTGGTTGCTGGTGTTTCCGTTTTATCTATCTATGCTATGGATACTCTTACAAGGATTAGATTCCTCTCCAATTGTTACACTTTTCAGTTCCCCAAAACCCCTGCATTATCTCCTTTCCCAACTGCTTTTTCAACAACCACACATTTTGTTTCACCACTAAGAGCGGCTCGTACCACCGCCACCACCACCAGTACCACCACCACAGTGAGCGATGTGGACGAGGAAGTCTTGCAAATATTTTTGAAGGAGAGACAATTAAATGGGGATTTTGTATCAAGAACTACTGATTTAATATGGCAAATGAACTTCAGAAACTCTGGTGATTATGACGTTAGTGAGCTCACCGACAACTATTCTCAACAAACAGAACAGATCACAGAGAGTAACAGTGATGACGGTTTTTTGAAACTTTCAAGTACCCAAGAATGGTTATTAGGTGACAATTCTGCACCAATAAACAGGAAGATGACTGCCAAGACAATCCAAGACAACAGAGCAAGACTCAAGAAACTGGAGGCACTCAAATATCAATCTCTCAAGAGAGAACTACTACTTCTCTCTGTGGGTATTGGATTGGCTTGTAGTGGATATTGCTTGGTAGTTTTTTCTGTACAGGCTGCTTTAAGTTATGCGGTTGGAGTCCTTTTCAGTTGCTTGTACCTTCAGCTCTTGTATCAACATGCAGACAACATATCAAGTGAAAACGTTCCTCAAATATTCAGGAAAAAGAAGTCGAACAAAATTGGCATAAGAAGCGAGGACCTTAATGATTTTTTCGAGAAAACAATCAGGGGTAGCGGTATATCACTTTCATCTCCAAGGCTCGTGATCCCAGCAGCAATTTATGGACTTTGGGCTTTGTCTCATCAGTATTTTACCAATGACTTGTTTGATTTTCAGCTTGTGCCAGCCATGGTTGGTATGTTTGTCTACAAGGCTGCTGCTCTAGTGCAAGTTTACAGAGACAATAAAGAGTTAGGCTTTGAATTTCCAGAAGATTATTAATGAAGGAAGGCTCAAGTGACTGAAATATTTATTTCGCTATGTTCAGCAGATCACTCAATTTCCTACATCCTCAAATATTGGAAATGGCCTGCTGCATCCATAGCTGAGCCCTACTTACAGTTATCTTGCACCAAAAAAGTGTCATATAATATATAGAAGATGATTTGAAATGAATAAAAGTACCCATTATGTAGGAGAGTTGCTAAGATGCAA
TARGET=263,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6463
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.612170
PRIMER_LEFT_SEQUENCE=CTGCATTATCTCCTTTCCCAAC
PRIMER_RIGHT_SEQUENCE=TATTTGCAAGACTTCCTCGTCC
PRIMER_LEFT=184,22
PRIMER_RIGHT=320,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.612
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=0.6463
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.612170
PRIMER_LEFT_1_SEQUENCE=CATTATCTCCTTTCCCAACTGC
PRIMER_RIGHT_1_SEQUENCE=TATTTGCAAGACTTCCTCGTCC
PRIMER_LEFT_1=187,22
PRIMER_RIGHT_1=320,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.612
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=0.6463
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.612170
PRIMER_LEFT_2_SEQUENCE=TGCATTATCTCCTTTCCCAACT
PRIMER_RIGHT_2_SEQUENCE=TATTTGCAAGACTTCCTCGTCC
PRIMER_LEFT_2=185,22
PRIMER_RIGHT_2=320,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=60.612
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.6463
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.612170
PRIMER_LEFT_3_SEQUENCE=GCATTATCTCCTTTCCCAACTG
PRIMER_RIGHT_3_SEQUENCE=TATTTGCAAGACTTCCTCGTCC
PRIMER_LEFT_3=186,22
PRIMER_RIGHT_3=320,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=60.612
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=0.6515
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.617395
PRIMER_LEFT_4_SEQUENCE=CTGCATTATCTCCTTTCCCAAC
PRIMER_RIGHT_4_SEQUENCE=TTGAGAATAGTTGTCGGTGAGC
PRIMER_LEFT_4=184,22
PRIMER_RIGHT_4=443,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=59.383
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=260
=
PRIMER_SEQUENCE_ID=SSR_05077 Ps_contig_mira-and-tgicl-ass_franssen_36411
SEQUENCE=AACTCTAGACACTACTACNNACACCCCCGCGCTTATTTATCATTCTTCCCACTCTACTACCCANACCCCCGCGCTTATTTATCATTCTTCCCACTCCAACCCTAATTTAATTATCACTCTAATTCCATGGCTTCACCCCACAATTTCTTCAAGAGAAAAGCCACCGGCACCGTCGATCAAATCAGCCACGATTTCTCCGATTTCTCTCTTTCTTCTCCGGCCACCAAAATCCGTCGCCTAGATGCTGAATTGACTCCAATTGTCGAAGAAGAAGAAAACGAACCCTTAACTCTTCCTAACGACGAGAGAGCTATTGTACTCTTCAAACCATCTCTTCACTCTTCTCCCTCTTTCTCTCTCACTCTCAATTCCGATCTTATCTCTCAAATTAAGAATAATCAAGTTTCGTGGTCAAAACAATGTGATGATTATCATGATAACCTAATTGAGCAAAACGACAATAATGATCGTCGTTTAGCTATTGTGCCTTGGGTACCACAATCTTCCTCTGGTTCTTCTTCCCATGCTTTTGATGATGATGATAACAACAATACAAATACTATAGAGTTGATGGAAGATGATGAAATGGGACAAGAACGAGAAGATGAAGATGAAGGAGCTATGATGGATGTAGAAGAAGAACAACAACAAGACAATAAGTCTTCGAGTTTTAACTATCCAACTATGGTTGAGGGGTTTCAGCAACACTGTTTGTTCCCTCAGATTATTCCTCAAAACACTTCATCTCCTATCACTTGGACTCGTTGAAAAGCATTACCTAGTTAGTTACTATACCTAGCTCCTTGACACCTCTGTCGGCTTGTGTTCGGGGTGCGGGCTTGATAGATAGTTAACTATCTTTAATGTCTTGATCTTTTTAGGATGCTAGGTTACACTAGACAATACAACAGATTTTAGTTTTGCTGTAGATAGAAAGTNNTTTTTTTTTGTTGTTGTTTTGGGTTAGGAAAGAAGTGATTAGGAGAAGTTGGTCAAATGGGTATGTTGGTGACTAAGATGGATGGATGATGGAGTTCCTTGGATTTTTCCATAAGACCCTTTCAAGTAACAATGAAAATCAATGTTTTATTTGTAAGTTTAAAAAT
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5020
PRIMER_LEFT_PENALTY=0.117817
PRIMER_RIGHT_PENALTY=0.384229
PRIMER_LEFT_SEQUENCE=TGCTGAATTGACTCCAATTGTC
PRIMER_RIGHT_SEQUENCE=AAGAACCAGAGGAAGATTGTGG
PRIMER_LEFT=242,22
PRIMER_RIGHT=516,22
PRIMER_LEFT_TM=60.118
PRIMER_RIGHT_TM=59.616
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.5525
PRIMER_LEFT_1_PENALTY=0.117817
PRIMER_RIGHT_1_PENALTY=0.434710
PRIMER_LEFT_1_SEQUENCE=TGCTGAATTGACTCCAATTGTC
PRIMER_RIGHT_1_SEQUENCE=ACCCAAGGCACAATAGCTAAAC
PRIMER_LEFT_1=242,22
PRIMER_RIGHT_1=493,22
PRIMER_LEFT_1_TM=60.118
PRIMER_RIGHT_1_TM=59.565
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.6202
PRIMER_LEFT_2_PENALTY=0.235991
PRIMER_RIGHT_2_PENALTY=0.384229
PRIMER_LEFT_2_SEQUENCE=GCCTAGATGCTGAATTGACTCC
PRIMER_RIGHT_2_SEQUENCE=AAGAACCAGAGGAAGATTGTGG
PRIMER_LEFT_2=235,22
PRIMER_RIGHT_2=516,22
PRIMER_LEFT_2_TM=60.236
PRIMER_RIGHT_2_TM=59.616
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.6707
PRIMER_LEFT_3_PENALTY=0.235991
PRIMER_RIGHT_3_PENALTY=0.434710
PRIMER_LEFT_3_SEQUENCE=GCCTAGATGCTGAATTGACTCC
PRIMER_RIGHT_3_SEQUENCE=ACCCAAGGCACAATAGCTAAAC
PRIMER_LEFT_3=235,22
PRIMER_RIGHT_3=493,22
PRIMER_LEFT_3_TM=60.236
PRIMER_RIGHT_3_TM=59.565
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.7105
PRIMER_LEFT_4_PENALTY=0.117817
PRIMER_RIGHT_4_PENALTY=0.592680
PRIMER_LEFT_4_SEQUENCE=TGCTGAATTGACTCCAATTGTC
PRIMER_RIGHT_4_SEQUENCE=TCAAAAGCATGGGAAGAAGAAC
PRIMER_LEFT_4=242,22
PRIMER_RIGHT_4=532,22
PRIMER_LEFT_4_TM=60.118
PRIMER_RIGHT_4_TM=60.593
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_05078 Ps_contig_mira-and-tgicl-ass_franssen_36411
SEQUENCE=AACTCTAGACACTACTACNNACACCCCCGCGCTTATTTATCATTCTTCCCACTCTACTACCCANACCCCCGCGCTTATTTATCATTCTTCCCACTCCAACCCTAATTTAATTATCACTCTAATTCCATGGCTTCACCCCACAATTTCTTCAAGAGAAAAGCCACCGGCACCGTCGATCAAATCAGCCACGATTTCTCCGATTTCTCTCTTTCTTCTCCGGCCACCAAAATCCGTCGCCTAGATGCTGAATTGACTCCAATTGTCGAAGAAGAAGAAAACGAACCCTTAACTCTTCCTAACGACGAGAGAGCTATTGTACTCTTCAAACCATCTCTTCACTCTTCTCCCTCTTTCTCTCTCACTCTCAATTCCGATCTTATCTCTCAAATTAAGAATAATCAAGTTTCGTGGTCAAAACAATGTGATGATTATCATGATAACCTAATTGAGCAAAACGACAATAATGATCGTCGTTTAGCTATTGTGCCTTGGGTACCACAATCTTCCTCTGGTTCTTCTTCCCATGCTTTTGATGATGATGATAACAACAATACAAATACTATAGAGTTGATGGAAGATGATGAAATGGGACAAGAACGAGAAGATGAAGATGAAGGAGCTATGATGGATGTAGAAGAAGAACAACAACAAGACAATAAGTCTTCGAGTTTTAACTATCCAACTATGGTTGAGGGGTTTCAGCAACACTGTTTGTTCCCTCAGATTATTCCTCAAAACACTTCATCTCCTATCACTTGGACTCGTTGAAAAGCATTACCTAGTTAGTTACTATACCTAGCTCCTTGACACCTCTGTCGGCTTGTGTTCGGGGTGCGGGCTTGATAGATAGTTAACTATCTTTAATGTCTTGATCTTTTTAGGATGCTAGGTTACACTAGACAATACAACAGATTTTAGTTTTGCTGTAGATAGAAAGTNNTTTTTTTTTGTTGTTGTTTTGGGTTAGGAAAGAAGTGATTAGGAGAAGTTGGTCAAATGGGTATGTTGGTGACTAAGATGGATGGATGATGGAGTTCCTTGGATTTTTCCATAAGACCCTTTCAAGTAACAATGAAAATCAATGTTTTATTTGTAAGTTTAAAAAT
TARGET=531,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1432
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=0.007205
PRIMER_LEFT_SEQUENCE=TTGGGTACCACAATCTTCCTCT
PRIMER_RIGHT_SEQUENCE=GGTAATGCTTTTCAACGAGTCC
PRIMER_LEFT=488,22
PRIMER_RIGHT=778,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.007
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.1432
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=0.007205
PRIMER_LEFT_1_SEQUENCE=CTTGGGTACCACAATCTTCCTC
PRIMER_RIGHT_1_SEQUENCE=GGTAATGCTTTTCAACGAGTCC
PRIMER_LEFT_1=487,22
PRIMER_RIGHT_1=778,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.007
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.1602
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.024206
PRIMER_LEFT_2_SEQUENCE=TTGGGTACCACAATCTTCCTCT
PRIMER_RIGHT_2_SEQUENCE=GTCCCATTTCATCATCTTCCAT
PRIMER_LEFT_2=488,22
PRIMER_RIGHT_2=591,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.024
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=0.1602
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.024206
PRIMER_LEFT_3_SEQUENCE=CTTGGGTACCACAATCTTCCTC
PRIMER_RIGHT_3_SEQUENCE=GTCCCATTTCATCATCTTCCAT
PRIMER_LEFT_3=487,22
PRIMER_RIGHT_3=591,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=60.024
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.2455
PRIMER_LEFT_4_PENALTY=0.136017
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=TTGGGTACCACAATCTTCCTCT
PRIMER_RIGHT_4_SEQUENCE=CTTCTCGTTCTTGTCCCATTTC
PRIMER_LEFT_4=488,22
PRIMER_RIGHT_4=603,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=116
=
PRIMER_SEQUENCE_ID=SSR_05079 Ps_contig_mira-and-tgicl-ass_franssen_36411
SEQUENCE=AACTCTAGACACTACTACNNACACCCCCGCGCTTATTTATCATTCTTCCCACTCTACTACCCANACCCCCGCGCTTATTTATCATTCTTCCCACTCCAACCCTAATTTAATTATCACTCTAATTCCATGGCTTCACCCCACAATTTCTTCAAGAGAAAAGCCACCGGCACCGTCGATCAAATCAGCCACGATTTCTCCGATTTCTCTCTTTCTTCTCCGGCCACCAAAATCCGTCGCCTAGATGCTGAATTGACTCCAATTGTCGAAGAAGAAGAAAACGAACCCTTAACTCTTCCTAACGACGAGAGAGCTATTGTACTCTTCAAACCATCTCTTCACTCTTCTCCCTCTTTCTCTCTCACTCTCAATTCCGATCTTATCTCTCAAATTAAGAATAATCAAGTTTCGTGGTCAAAACAATGTGATGATTATCATGATAACCTAATTGAGCAAAACGACAATAATGATCGTCGTTTAGCTATTGTGCCTTGGGTACCACAATCTTCCTCTGGTTCTTCTTCCCATGCTTTTGATGATGATGATAACAACAATACAAATACTATAGAGTTGATGGAAGATGATGAAATGGGACAAGAACGAGAAGATGAAGATGAAGGAGCTATGATGGATGTAGAAGAAGAACAACAACAAGACAATAAGTCTTCGAGTTTTAACTATCCAACTATGGTTGAGGGGTTTCAGCAACACTGTTTGTTCCCTCAGATTATTCCTCAAAACACTTCATCTCCTATCACTTGGACTCGTTGAAAAGCATTACCTAGTTAGTTACTATACCTAGCTCCTTGACACCTCTGTCGGCTTGTGTTCGGGGTGCGGGCTTGATAGATAGTTAACTATCTTTAATGTCTTGATCTTTTTAGGATGCTAGGTTACACTAGACAATACAACAGATTTTAGTTTTGCTGTAGATAGAAAGTNNTTTTTTTTTGTTGTTGTTTTGGGTTAGGAAAGAAGTGATTAGGAGAAGTTGGTCAAATGGGTATGTTGGTGACTAAGATGGATGGATGATGGAGTTCCTTGGATTTTTCCATAAGACCCTTTCAAGTAACAATGAAAATCAATGTTTTATTTGTAAGTTTAAAAAT
TARGET=1017,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1980
PRIMER_LEFT_PENALTY=0.061961
PRIMER_RIGHT_PENALTY=1.136032
PRIMER_LEFT_SEQUENCE=CCTAGCTCCTTGACACCTCTGT
PRIMER_RIGHT_SEQUENCE=GGGTCTTATGGAAAAATCCAAG
PRIMER_LEFT=794,22
PRIMER_RIGHT=1058,22
PRIMER_LEFT_TM=59.938
PRIMER_RIGHT_TM=58.864
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=1.1980
PRIMER_LEFT_1_PENALTY=0.061961
PRIMER_RIGHT_1_PENALTY=1.136032
PRIMER_LEFT_1_SEQUENCE=ACCTAGCTCCTTGACACCTCTG
PRIMER_RIGHT_1_SEQUENCE=GGGTCTTATGGAAAAATCCAAG
PRIMER_LEFT_1=793,22
PRIMER_RIGHT_1=1058,22
PRIMER_LEFT_1_TM=59.938
PRIMER_RIGHT_1_TM=58.864
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=1.1980
PRIMER_LEFT_2_PENALTY=0.061961
PRIMER_RIGHT_2_PENALTY=1.136032
PRIMER_LEFT_2_SEQUENCE=CCTAGCTCCTTGACACCTCTGT
PRIMER_RIGHT_2_SEQUENCE=GGTCTTATGGAAAAATCCAAGG
PRIMER_LEFT_2=794,22
PRIMER_RIGHT_2=1057,22
PRIMER_LEFT_2_TM=59.938
PRIMER_RIGHT_2_TM=58.864
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=264
PRIMER_PAIR_PENALTY_3=1.1980
PRIMER_LEFT_3_PENALTY=0.061961
PRIMER_RIGHT_3_PENALTY=1.136032
PRIMER_LEFT_3_SEQUENCE=ACCTAGCTCCTTGACACCTCTG
PRIMER_RIGHT_3_SEQUENCE=GGTCTTATGGAAAAATCCAAGG
PRIMER_LEFT_3=793,22
PRIMER_RIGHT_3=1057,22
PRIMER_LEFT_3_TM=59.938
PRIMER_RIGHT_3_TM=58.864
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=265
PRIMER_PAIR_PENALTY_4=1.2921
PRIMER_LEFT_4_PENALTY=0.007205
PRIMER_RIGHT_4_PENALTY=1.284927
PRIMER_LEFT_4_SEQUENCE=GGACTCGTTGAAAAGCATTACC
PRIMER_RIGHT_4_SEQUENCE=GGAAAAATCCAAGGAACTCCA
PRIMER_LEFT_4=757,22
PRIMER_RIGHT_4=1049,21
PRIMER_LEFT_4_TM=60.007
PRIMER_RIGHT_4_TM=60.285
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_05080 Ps_contig_mira-and-tgicl-ass_franssen_36415
SEQUENCE=CGTCGCCAAAGNTTTTTGACATCAACATCATGAAAAAATATATATATTAGAATCCTTTAAAGTGAGCTGAACAACTATGGTGAAATCTAGACAGATTGCTTCAATTAAAGTTATATAAACAGACACAGGAAAATCATTTAGCAGTCTATCAGTCTTCTATGACTCTGCTTCGCATTTTTTCTCCGTAAACTCCATCTCTCTTTAATGTCATTTGTGTTAGCAACCAGGTCAAAACTTTTATCCGTTTTTTCTTGAATTTCTTCCTTGGACTTTTCAGTCTCATATCCTCCTCTGATTCTGGCAGTTTCTGCTTGCAAATCAGTTATAAGCATTTCAGTTGTCTTGGCAGTGATTCCTCTCAGATACCATGATATGGGAGGTGGAGGGACAATCGCAGGTTGAAGTATTTGTTCTAGACGAACTTTAGACCCGATTCTTCCTTTGCCTCTTGTACACATGTTATAGTCTTCTTTTGTTAAGGGTTTAAAGGGATAACAGGTTGCTTCATCGACAAATAAAGGCTCGACTCTCCAGTGGCCCTCAAATTTTCTCATGAATCCTGTCTTGGTTTGCTTGAACTTCATAGAGTAGTCATTTCTGTTTTGATCAACCAAAACATTTATTGAAATAGTTCCAGACCACCAAAGAAATTTCCAAATAGCTGCTTGTTCAAGATCAACCACTTGTCTATGACCCTCATCAACCAAAACTTTCCTGGATATAACTTCTTTGATATTTTTGAAAACTCTTCTATTGTCAGGATCTGTTACTATATTATATACTGCATCAGGGGGAAAACCAACATCAACTTCTACATTGAGGTTACAAAGAGAGCCTTTGGGAACAGTCACCTTTATTTCTGATGGCTTATCAACCCAAGAAGGATTTTCTTTCCATGCTTGCAGTTGCTCATTCAGTTCAGCTTCAGAAACAGTAAAAGTTCCACTTCCATGTCTCTGAACAGATCTCCCCCAGTTCATTCCCTCGTGTTTTGCTAATCTACTCAGCTGATTCTTTACAACATTTTGAAACTTGTGAGGGAGCTGTACAAAAAGTTTAGATATTGCAGGATTCAATTCTTTTTTTAAACCCATCTTTTTCATTCTTCCTGACATCTAAGCCGAACTTTGTACCCTCTGACTCGCCCATCATAAGAACAAGGTAAAAAGTATGATGAAGCAAGAACTTGACTTCAAAGTTGGAACTTTGGAATTTGAAAAGTATGGTTGAAGAATGGGCGACGAAGATAAGTAAGATGAAACCACGTGCTATACATGTTCAATTTGTCTGCGACCCATCANNCRCNTACTCTGCGTTGGAAATTACCGAAACTGACTT
TARGET=38,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05081 Ps_contig_mira-and-tgicl-ass_franssen_36417
SEQUENCE=TCTCACCAATTGTACCTGCCAAGTACGCTTACCTGTACCTCCCACCCACACTTCTCGCATCATCACCTTTCCATTCTCTTCACTATTAATTAATTCAATTCCCCTTTTCATCTTCACATATATATAAACCACTCTCACTCTCTCCTTCTCTTCTATTCTATTATCATTCCTTTTCACTCTTCACTTCTCAACCACTTCAACTTTCTCTCTCTAGATTCCTCATCTTCTCATCATGAAGCAGTTGATTCGCCGATTATCTCGAGTCGCTGACTCTTCAACCTACACGCTACTCCGAACAGATTCATCAACAACAACAACAACTCGTCGCCGGCGACCTTCCCGCCGAGTCGAATCTTTCCGGATGGAAGTATTATCGAAGATCCGGAGATCTTCCACGGTTGTTCCGGAAGGACACGTCCCGATCTACGTCGGAGACGAGATGGAGCGGTTCGTCGTGTGCGCCGAGCTTCTCAACCATCCGATTTTCGTCAAGCTTTTGAACGAATCGGCGCAGGAATACGGTTACGAACAGAAAGGCGTGCTTCGGTTACCGTGCCACGTGTTCGTTTTCGAGCGCGTGCTTGACGCGCTAAAACATGGACGCGACACGCGCTGTATCGCTGAGCTTCTAAATTCTTCTTCTGAAGAAGGATTATGCTGATGGAGTTTAAAATTAGGTAAATTAGTGAGTTTTTTTTTGTTATTATGTAAATATGAATCAGGATCTTGAGAGAGAGAAGAAAACAAAAAACAAAAAGGAGAAAGGAAATGTTGTTGTTAGGGTTTGTTTTGTGTCTCTTGTTTTTTGTTTGTTGGTTGTTGTTGTTGAGTGTAAAGAGAAGATTATTTATTATTATTATTACTCTTCTTATGTTTGCAAACCGAAAGG
TARGET=306,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3471
PRIMER_LEFT_PENALTY=0.185822
PRIMER_RIGHT_PENALTY=0.161322
PRIMER_LEFT_SEQUENCE=CAATTCCCCTTTTCATCTTCAC
PRIMER_RIGHT_SEQUENCE=CGATAATACTTCCATCCGGAAA
PRIMER_LEFT=95,22
PRIMER_RIGHT=375,22
PRIMER_LEFT_TM=59.814
PRIMER_RIGHT_TM=60.161
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.5930
PRIMER_LEFT_1_PENALTY=0.158297
PRIMER_RIGHT_1_PENALTY=0.434710
PRIMER_LEFT_1_SEQUENCE=ACGCTACTCCGAACAGATTCAT
PRIMER_RIGHT_1_SEQUENCE=GCCTTTCTGTTCGTAACCGTAT
PRIMER_LEFT_1=283,22
PRIMER_RIGHT_1=537,22
PRIMER_LEFT_1_TM=60.158
PRIMER_RIGHT_1_TM=59.565
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=0.6178
PRIMER_LEFT_2_PENALTY=0.456523
PRIMER_RIGHT_2_PENALTY=0.161322
PRIMER_LEFT_2_SEQUENCE=TCGCTGACTCTTCAACCTACAC
PRIMER_RIGHT_2_SEQUENCE=CGATAATACTTCCATCCGGAAA
PRIMER_LEFT_2=263,22
PRIMER_RIGHT_2=375,22
PRIMER_LEFT_2_TM=59.543
PRIMER_RIGHT_2_TM=60.161
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=0.6178
PRIMER_LEFT_3_PENALTY=0.456523
PRIMER_RIGHT_3_PENALTY=0.161322
PRIMER_LEFT_3_SEQUENCE=GTCGCTGACTCTTCAACCTACA
PRIMER_RIGHT_3_SEQUENCE=CGATAATACTTCCATCCGGAAA
PRIMER_LEFT_3=262,22
PRIMER_RIGHT_3=375,22
PRIMER_LEFT_3_TM=59.543
PRIMER_RIGHT_3_TM=60.161
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=0.8912
PRIMER_LEFT_4_PENALTY=0.456523
PRIMER_RIGHT_4_PENALTY=0.434710
PRIMER_LEFT_4_SEQUENCE=GTCGCTGACTCTTCAACCTACA
PRIMER_RIGHT_4_SEQUENCE=GCCTTTCTGTTCGTAACCGTAT
PRIMER_LEFT_4=262,22
PRIMER_RIGHT_4=537,22
PRIMER_LEFT_4_TM=59.543
PRIMER_RIGHT_4_TM=59.565
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_05082 Ps_contig_mira-and-tgicl-ass_franssen_36417
SEQUENCE=TCTCACCAATTGTACCTGCCAAGTACGCTTACCTGTACCTCCCACCCACACTTCTCGCATCATCACCTTTCCATTCTCTTCACTATTAATTAATTCAATTCCCCTTTTCATCTTCACATATATATAAACCACTCTCACTCTCTCCTTCTCTTCTATTCTATTATCATTCCTTTTCACTCTTCACTTCTCAACCACTTCAACTTTCTCTCTCTAGATTCCTCATCTTCTCATCATGAAGCAGTTGATTCGCCGATTATCTCGAGTCGCTGACTCTTCAACCTACACGCTACTCCGAACAGATTCATCAACAACAACAACAACTCGTCGCCGGCGACCTTCCCGCCGAGTCGAATCTTTCCGGATGGAAGTATTATCGAAGATCCGGAGATCTTCCACGGTTGTTCCGGAAGGACACGTCCCGATCTACGTCGGAGACGAGATGGAGCGGTTCGTCGTGTGCGCCGAGCTTCTCAACCATCCGATTTTCGTCAAGCTTTTGAACGAATCGGCGCAGGAATACGGTTACGAACAGAAAGGCGTGCTTCGGTTACCGTGCCACGTGTTCGTTTTCGAGCGCGTGCTTGACGCGCTAAAACATGGACGCGACACGCGCTGTATCGCTGAGCTTCTAAATTCTTCTTCTGAAGAAGGATTATGCTGATGGAGTTTAAAATTAGGTAAATTAGTGAGTTTTTTTTTGTTATTATGTAAATATGAATCAGGATCTTGAGAGAGAGAAGAAAACAAAAAACAAAAAGGAGAAAGGAAATGTTGTTGTTAGGGTTTGTTTTGTGTCTCTTGTTTTTTGTTTGTTGGTTGTTGTTGTTGAGTGTAAAGAGAAGATTATTTATTATTATTATTACTCTTCTTATGTTTGCAAACCGAAAGG
TARGET=729,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4992
PRIMER_LEFT_PENALTY=1.052316
PRIMER_RIGHT_PENALTY=0.446858
PRIMER_LEFT_SEQUENCE=GCTGTATCGCTGAGCTTCTAAA
PRIMER_RIGHT_SEQUENCE=CACTCAACAACAACAACCAACA
PRIMER_LEFT=611,22
PRIMER_RIGHT=831,22
PRIMER_LEFT_TM=58.948
PRIMER_RIGHT_TM=59.553
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=2.1566
PRIMER_LEFT_1_PENALTY=0.434710
PRIMER_RIGHT_1_PENALTY=1.721904
PRIMER_LEFT_1_SEQUENCE=ATACGGTTACGAACAGAAAGGC
PRIMER_RIGHT_1_SEQUENCE=CAAGAGACACAAAACAAACCCT
PRIMER_LEFT_1=516,22
PRIMER_RIGHT_1=800,22
PRIMER_LEFT_1_TM=59.565
PRIMER_RIGHT_1_TM=58.278
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=2.1691
PRIMER_LEFT_2_PENALTY=1.722217
PRIMER_RIGHT_2_PENALTY=0.446858
PRIMER_LEFT_2_SEQUENCE=GAAGAAGGATTATGCTGATGGA
PRIMER_RIGHT_2_SEQUENCE=CACTCAACAACAACAACCAACA
PRIMER_LEFT_2=643,22
PRIMER_RIGHT_2=831,22
PRIMER_LEFT_2_TM=58.278
PRIMER_RIGHT_2_TM=59.553
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=2.2035
PRIMER_LEFT_3_PENALTY=1.756595
PRIMER_RIGHT_3_PENALTY=0.446858
PRIMER_LEFT_3_SEQUENCE=GCTGTATCGCTGAGCTTCTAAAT
PRIMER_RIGHT_3_SEQUENCE=CACTCAACAACAACAACCAACA
PRIMER_LEFT_3=611,23
PRIMER_RIGHT_3=831,22
PRIMER_LEFT_3_TM=59.243
PRIMER_RIGHT_3_TM=59.553
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=2.2105
PRIMER_LEFT_4_PENALTY=1.763688
PRIMER_RIGHT_4_PENALTY=0.446858
PRIMER_LEFT_4_SEQUENCE=GAAGAAGGATTATGCTGATGGAG
PRIMER_RIGHT_4_SEQUENCE=CACTCAACAACAACAACCAACA
PRIMER_LEFT_4=643,23
PRIMER_RIGHT_4=831,22
PRIMER_LEFT_4_TM=59.236
PRIMER_RIGHT_4_TM=59.553
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_05083 Ps_contig_mira-and-tgicl-ass_franssen_36417
SEQUENCE=TCTCACCAATTGTACCTGCCAAGTACGCTTACCTGTACCTCCCACCCACACTTCTCGCATCATCACCTTTCCATTCTCTTCACTATTAATTAATTCAATTCCCCTTTTCATCTTCACATATATATAAACCACTCTCACTCTCTCCTTCTCTTCTATTCTATTATCATTCCTTTTCACTCTTCACTTCTCAACCACTTCAACTTTCTCTCTCTAGATTCCTCATCTTCTCATCATGAAGCAGTTGATTCGCCGATTATCTCGAGTCGCTGACTCTTCAACCTACACGCTACTCCGAACAGATTCATCAACAACAACAACAACTCGTCGCCGGCGACCTTCCCGCCGAGTCGAATCTTTCCGGATGGAAGTATTATCGAAGATCCGGAGATCTTCCACGGTTGTTCCGGAAGGACACGTCCCGATCTACGTCGGAGACGAGATGGAGCGGTTCGTCGTGTGCGCCGAGCTTCTCAACCATCCGATTTTCGTCAAGCTTTTGAACGAATCGGCGCAGGAATACGGTTACGAACAGAAAGGCGTGCTTCGGTTACCGTGCCACGTGTTCGTTTTCGAGCGCGTGCTTGACGCGCTAAAACATGGACGCGACACGCGCTGTATCGCTGAGCTTCTAAATTCTTCTTCTGAAGAAGGATTATGCTGATGGAGTTTAAAATTAGGTAAATTAGTGAGTTTTTTTTTGTTATTATGTAAATATGAATCAGGATCTTGAGAGAGAGAAGAAAACAAAAAACAAAAAGGAGAAAGGAAATGTTGTTGTTAGGGTTTGTTTTGTGTCTCTTGTTTTTTGTTTGTTGGTTGTTGTTGTTGAGTGTAAAGAGAAGATTATTTATTATTATTATTACTCTTCTTATGTTTGCAAACCGAAAGG
TARGET=816,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05084 Ps_contig_mira-and-tgicl-ass_franssen_36417
SEQUENCE=TCTCACCAATTGTACCTGCCAAGTACGCTTACCTGTACCTCCCACCCACACTTCTCGCATCATCACCTTTCCATTCTCTTCACTATTAATTAATTCAATTCCCCTTTTCATCTTCACATATATATAAACCACTCTCACTCTCTCCTTCTCTTCTATTCTATTATCATTCCTTTTCACTCTTCACTTCTCAACCACTTCAACTTTCTCTCTCTAGATTCCTCATCTTCTCATCATGAAGCAGTTGATTCGCCGATTATCTCGAGTCGCTGACTCTTCAACCTACACGCTACTCCGAACAGATTCATCAACAACAACAACAACTCGTCGCCGGCGACCTTCCCGCCGAGTCGAATCTTTCCGGATGGAAGTATTATCGAAGATCCGGAGATCTTCCACGGTTGTTCCGGAAGGACACGTCCCGATCTACGTCGGAGACGAGATGGAGCGGTTCGTCGTGTGCGCCGAGCTTCTCAACCATCCGATTTTCGTCAAGCTTTTGAACGAATCGGCGCAGGAATACGGTTACGAACAGAAAGGCGTGCTTCGGTTACCGTGCCACGTGTTCGTTTTCGAGCGCGTGCTTGACGCGCTAAAACATGGACGCGACACGCGCTGTATCGCTGAGCTTCTAAATTCTTCTTCTGAAGAAGGATTATGCTGATGGAGTTTAAAATTAGGTAAATTAGTGAGTTTTTTTTTGTTATTATGTAAATATGAATCAGGATCTTGAGAGAGAGAAGAAAACAAAAAACAAAAAGGAGAAAGGAAATGTTGTTGTTAGGGTTTGTTTTGTGTCTCTTGTTTTTTGTTTGTTGGTTGTTGTTGTTGAGTGTAAAGAGAAGATTATTTATTATTATTATTACTCTTCTTATGTTTGCAAACCGAAAGG
TARGET=848,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05085 Ps_contig_mira-and-tgicl-ass_franssen_36419
SEQUENCE=GTTGTCTGTCGAAAGGACGTCCATTCGGTAGGGACGTTGTAAGGAATAACTACTCACCAAGGTACGGGTCCGATCGAGACTGCTGCCCCTACCAATAGAATACCAAGAGCTTCCTAGAGAGGAGGTGCAAGTACAAGATACTTCAAATGATATGTTGCCCCTTGAAGTAGAAATGAACATATTACAAGCTATATTGTACCATTGAGTTTTGGATGTTGTTTGTGACCATGATAGCCGGTTTGGGTTCTGGGCTTACAGTGATAAATAATATGAGCCAAATAGGAGAATCTTTTGGGTATAGTACTATTGAGATTAGCAATATGGTGTCATTGTGGAGTATGTGGAATTTTCTCGGGCGTTTTGGTGGCGGCCATGTATCCGATTATATCATGCACGAAAGAGGTTGGCCGCGGCCCCTGTTGTTGACTGTAACTCTGGGGATTACAATTCTTGGTCATTTAATCATAGCTACTGGTTTGCCTGGAAATTTTTATTTGGGTCCTGTCCTGGTGGGAATATGCTATGGTACAAATTGGTCCTTAATGCCAACAATCACTTCTGAGATATTTGGGGTGAAGCACATGGGTACCATTTTCAACGCGATTGCTGCAGCTAGTCCACTTGGGTCTTATATACTTTCTGTGAGAGTTGTAGGATATATATATGACAAGGAAGCCAATGAAGATAACTCATGCTTCGGCATCCACTGTTTCATGTTGTCGCTTCTGATCGTGGCCGGTGTATCTTTTCTAGCATTTTTGGTCAGTCTGGCATTGTATTTCCGGACCCGGAGATTCTATAAGCTAGTTGTGCTTAAGAAAATTAAACNTTATGCAAGATGATGAAGTAATTTTGTTTGCCAGCTATTTTANCATAA
TARGET=656,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1704
PRIMER_LEFT_PENALTY=0.149184
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=ATAGCTACTGGTTTGCCTGGAA
PRIMER_RIGHT_SEQUENCE=CTAGAAAAGATACACCGGCCAC
PRIMER_LEFT=464,22
PRIMER_RIGHT=752,22
PRIMER_LEFT_TM=60.149
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.2308
PRIMER_LEFT_1_PENALTY=0.209637
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=TTTTTATTTGGGTCCTGTCCTG
PRIMER_RIGHT_1_SEQUENCE=CTAGAAAAGATACACCGGCCAC
PRIMER_LEFT_1=487,22
PRIMER_RIGHT_1=752,22
PRIMER_LEFT_1_TM=60.210
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.2809
PRIMER_LEFT_2_PENALTY=0.259704
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=TGCCAACAATCACTTCTGAGAT
PRIMER_RIGHT_2_SEQUENCE=CTAGAAAAGATACACCGGCCAC
PRIMER_LEFT_2=543,22
PRIMER_RIGHT_2=752,22
PRIMER_LEFT_2_TM=59.740
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.2903
PRIMER_LEFT_3_PENALTY=0.269153
PRIMER_RIGHT_3_PENALTY=0.021195
PRIMER_LEFT_3_SEQUENCE=AGATATTTGGGGTGAAGCACAT
PRIMER_RIGHT_3_SEQUENCE=CTAGAAAAGATACACCGGCCAC
PRIMER_LEFT_3=561,22
PRIMER_RIGHT_3=752,22
PRIMER_LEFT_3_TM=59.731
PRIMER_RIGHT_3_TM=60.021
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=0.3021
PRIMER_LEFT_4_PENALTY=0.259704
PRIMER_RIGHT_4_PENALTY=0.042387
PRIMER_LEFT_4_SEQUENCE=TGCCAACAATCACTTCTGAGAT
PRIMER_RIGHT_4_SEQUENCE=AGCTTATAGAATCTCCGGGTCC
PRIMER_LEFT_4=543,22
PRIMER_RIGHT_4=804,22
PRIMER_LEFT_4_TM=59.740
PRIMER_RIGHT_4_TM=59.958
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_05086 Ps_contig_mira-and-tgicl-ass_franssen_36420
SEQUENCE=GGGGACACAATTACTCGGGCTAAGTGAGAGTGAGTGAGTGATCGCAATTATTATATAAAATATTTACATTCCTAGTAAGTGGGAGAGAGAGAGAGATGGCTAAGTGGTGTATGTTTTTGCTGGTTCTTGCTCTTGTTGTTGTTGCTGCAACAAGTGCAAGAGATGTACCAAGTGATGCTGGTCTCAAGGACCAGAAGAACTTTATGACATTTGGTGGTGGTTTTTATGGACTAGGAAACAACGGAATGCCATTTGGTGGAATCGGAGGTGGTATAGGTAGTGGTGGACTTGGTGGTGGTGCTGGTGGCTTTGGTGGTTTAGGAGGACTTGGTGGTGGAGCTGGAACTGATATAGGTGGTGGTGTTGGAAGTGGTGTTGGTGGTGGAAGTGGTGTTCTTCCTTTTCCTTAAGATCATCATCAACATTCAACAAAACGTGCATGGTTTTTCTTTAGTTTCTTTGAAATTGTTACTCTTAACTTTCAGTGTTTGTGTGTATGTGTTGTTTATCGTTTAAGCATTCTAGCTAGCTAGCATTTCTTGTTTATTAGTATTATTAGAAATTAGTGGTATCTCTTGTAAGTTGTAATGTTATTCTTTTTGNRAAAGTAATGACAAGTACTAAATTTTAATAAAAGGTTAAAAAAAAAGTTTT
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05087 Ps_contig_mira-and-tgicl-ass_franssen_36420
SEQUENCE=GGGGACACAATTACTCGGGCTAAGTGAGAGTGAGTGAGTGATCGCAATTATTATATAAAATATTTACATTCCTAGTAAGTGGGAGAGAGAGAGAGATGGCTAAGTGGTGTATGTTTTTGCTGGTTCTTGCTCTTGTTGTTGTTGCTGCAACAAGTGCAAGAGATGTACCAAGTGATGCTGGTCTCAAGGACCAGAAGAACTTTATGACATTTGGTGGTGGTTTTTATGGACTAGGAAACAACGGAATGCCATTTGGTGGAATCGGAGGTGGTATAGGTAGTGGTGGACTTGGTGGTGGTGCTGGTGGCTTTGGTGGTTTAGGAGGACTTGGTGGTGGAGCTGGAACTGATATAGGTGGTGGTGTTGGAAGTGGTGTTGGTGGTGGAAGTGGTGTTCTTCCTTTTCCTTAAGATCATCATCAACATTCAACAAAACGTGCATGGTTTTTCTTTAGTTTCTTTGAAATTGTTACTCTTAACTTTCAGTGTTTGTGTGTATGTGTTGTTTATCGTTTAAGCATTCTAGCTAGCTAGCATTTCTTGTTTATTAGTATTATTAGAAATTAGTGGTATCTCTTGTAAGTTGTAATGTTATTCTTTTTGNRAAAGTAATGACAAGTACTAAATTTTAATAAAAGGTTAAAAAAAAAGTTTT
TARGET=83,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05088 Ps_contig_mira-and-tgicl-ass_franssen_36420
SEQUENCE=GGGGACACAATTACTCGGGCTAAGTGAGAGTGAGTGAGTGATCGCAATTATTATATAAAATATTTACATTCCTAGTAAGTGGGAGAGAGAGAGAGATGGCTAAGTGGTGTATGTTTTTGCTGGTTCTTGCTCTTGTTGTTGTTGCTGCAACAAGTGCAAGAGATGTACCAAGTGATGCTGGTCTCAAGGACCAGAAGAACTTTATGACATTTGGTGGTGGTTTTTATGGACTAGGAAACAACGGAATGCCATTTGGTGGAATCGGAGGTGGTATAGGTAGTGGTGGACTTGGTGGTGGTGCTGGTGGCTTTGGTGGTTTAGGAGGACTTGGTGGTGGAGCTGGAACTGATATAGGTGGTGGTGTTGGAAGTGGTGTTGGTGGTGGAAGTGGTGTTCTTCCTTTTCCTTAAGATCATCATCAACATTCAACAAAACGTGCATGGTTTTTCTTTAGTTTCTTTGAAATTGTTACTCTTAACTTTCAGTGTTTGTGTGTATGTGTTGTTTATCGTTTAAGCATTCTAGCTAGCTAGCATTTCTTGTTTATTAGTATTATTAGAAATTAGTGGTATCTCTTGTAAGTTGTAATGTTATTCTTTTTGNRAAAGTAATGACAAGTACTAAATTTTAATAAAAGGTTAAAAAAAAAGTTTT
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05089 Ps_contig_mira-and-tgicl-ass_franssen_36420
SEQUENCE=GGGGACACAATTACTCGGGCTAAGTGAGAGTGAGTGAGTGATCGCAATTATTATATAAAATATTTACATTCCTAGTAAGTGGGAGAGAGAGAGAGATGGCTAAGTGGTGTATGTTTTTGCTGGTTCTTGCTCTTGTTGTTGTTGCTGCAACAAGTGCAAGAGATGTACCAAGTGATGCTGGTCTCAAGGACCAGAAGAACTTTATGACATTTGGTGGTGGTTTTTATGGACTAGGAAACAACGGAATGCCATTTGGTGGAATCGGAGGTGGTATAGGTAGTGGTGGACTTGGTGGTGGTGCTGGTGGCTTTGGTGGTTTAGGAGGACTTGGTGGTGGAGCTGGAACTGATATAGGTGGTGGTGTTGGAAGTGGTGTTGGTGGTGGAAGTGGTGTTCTTCCTTTTCCTTAAGATCATCATCAACATTCAACAAAACGTGCATGGTTTTTCTTTAGTTTCTTTGAAATTGTTACTCTTAACTTTCAGTGTTTGTGTGTATGTGTTGTTTATCGTTTAAGCATTCTAGCTAGCTAGCATTTCTTGTTTATTAGTATTATTAGAAATTAGTGGTATCTCTTGTAAGTTGTAATGTTATTCTTTTTGNRAAAGTAATGACAAGTACTAAATTTTAATAAAAGGTTAAAAAAAAAGTTTT
TARGET=522,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05090 Ps_contig_mira-and-tgicl-ass_franssen_36425
SEQUENCE=TCGGGGAGTGCAGCCTTTGRCGGTCGTTTGATCCTTAACTCAACAGAACTGCTTTCTCACGACGGTCGTGTTCCTCTACATTTTCCAGTTCAATTCTTCACACACAAAAACATTTGCTCATATATTCTCTCTCTCACTAACTTCAACAATCTCAATTTCTTCTTTCTAATCCAACCAAATTTCACACTCACACTCACACTCGTCAAATTCAAACCAAAAATGTCTGTTTTTTCCCCTCCAATACATTCACCACTTCTTCTAAACACCCAAACCAATCTTTCTTCATCTTCTTCACCTTCTTATCTTCTACCTCTTCTCACTTTCTCATCCAAACGCTCTTCTTCACTGACCCTTTTCTCTTCTTCAAAATCTGGAGATGGGTTGGCGAGTAAAGATAAAAAAATCTTACTTGAACAGTATGGTTATGATATCGACGCTGATGACTGCTTTTCTCAATCATCTCCAAAGTCCAAGAGGAAAAAGGTTCAACAAAAGACAAAAGGTGGAGGGAAGCAAGTACAAGATCCACCCGAGGAACCTAAGCCTCCTCGTACTACACATAAATTGCTTCAGGTTATTGGAGGAACAGCTCGAAGAACGAAGCTACTCTCACCAAACAGCATGGATGTACGCCCCATGATGGAGGTCGTGAAAGGTGCAGCCTTTGATATATTACAGTCGGCTTGTGGTTCTCCTGCATCCTTGAGACCTGGACGCTGGTTAGACTTGTATAGCGGTACCGGTTCTGTTGGAATTGAAGCACTTAGCCGAGGATGTTCTGAGGTGCATTTTGTTGAGATGGACCCTTGGGTTGTATCGGATGTTTTACGTCCAAACTTAGAGGTGACCAGATTCGTCGATGATTCAGTCATTCACACCGTTCGCGTGGAGAAATTCTTCGAACTCGCAGAGCAATTTGTAGGAAACAAGAGCACATTCGATTACATCAGCGTCACCCCTCCATACGAACAAGTTGACTATGTGGTGCTAATAGAGARTGATTCGGAATCTACCG
TARGET=126,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05091 Ps_contig_mira-and-tgicl-ass_franssen_36427
SEQUENCE=TCCAAATCAAACTTTCCAACAAGACCTTTCTTCACCATTCTCAAACAAACAACTTCATCTTCATCATGGTCTCTTGCAACTTCCTGATCTTCAAGGAAACACCAATAATAACACGAATTCATCATCGGTGTCGCCTTTTAACCTAAGCTTCTTTCCGAATACCAATAGCAGCACAATGCATGATCAGTTCAACAACATAAGCGAACCTCATCATCATCATCATCATCATCATCATCACCAGCAGCATCACCAAGAAGGCTTTTCTTCACTCTTTGGTAATTCATCAATGCAACAAGAGAACATGTTGTCCTCACCGCACATGTCAGCAACTGCATTGCTTCAAAAAGCTGCTCAAATAGGTTCAACAACAACAACTACTATCAATAACAACGGTGTGGAATCTAATAATGAAGGATTAAGATCAAGCATGGAAAATGAACACAACCAACATCTACATGGATTGCTAAACT
TARGET=208,30
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2446
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=0.237699
PRIMER_LEFT_SEQUENCE=TCTTGCAACTTCCTGATCTTCA
PRIMER_RIGHT_SEQUENCE=AAGAAAAGCCTTCTTGGTGATG
PRIMER_LEFT=71,22
PRIMER_RIGHT=265,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.762
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=0.2817
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=0.274768
PRIMER_LEFT_1_SEQUENCE=TCTTGCAACTTCCTGATCTTCA
PRIMER_RIGHT_1_SEQUENCE=GTGAAGAAAAGCCTTCTTGGTG
PRIMER_LEFT_1=71,22
PRIMER_RIGHT_1=268,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=60.275
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.3527
PRIMER_LEFT_2_PENALTY=0.115007
PRIMER_RIGHT_2_PENALTY=0.237699
PRIMER_LEFT_2_SEQUENCE=CTTTCCGAATACCAATAGCAGC
PRIMER_RIGHT_2_SEQUENCE=AAGAAAAGCCTTCTTGGTGATG
PRIMER_LEFT_2=150,22
PRIMER_RIGHT_2=265,22
PRIMER_LEFT_2_TM=60.115
PRIMER_RIGHT_2_TM=59.762
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=0.3898
PRIMER_LEFT_3_PENALTY=0.115007
PRIMER_RIGHT_3_PENALTY=0.274768
PRIMER_LEFT_3_SEQUENCE=CTTTCCGAATACCAATAGCAGC
PRIMER_RIGHT_3_SEQUENCE=GTGAAGAAAAGCCTTCTTGGTG
PRIMER_LEFT_3=150,22
PRIMER_RIGHT_3=268,22
PRIMER_LEFT_3_TM=60.115
PRIMER_RIGHT_3_TM=60.275
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=0.4856
PRIMER_LEFT_4_PENALTY=0.006945
PRIMER_RIGHT_4_PENALTY=0.478666
PRIMER_LEFT_4_SEQUENCE=TCTTGCAACTTCCTGATCTTCA
PRIMER_RIGHT_4_SEQUENCE=GAGTGAAGAAAAGCCTTCTTGG
PRIMER_LEFT_4=71,22
PRIMER_RIGHT_4=270,22
PRIMER_LEFT_4_TM=59.993
PRIMER_RIGHT_4_TM=59.521
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_05092 Ps_contig_mira-and-tgicl-ass_franssen_36427
SEQUENCE=TCCAAATCAAACTTTCCAACAAGACCTTTCTTCACCATTCTCAAACAAACAACTTCATCTTCATCATGGTCTCTTGCAACTTCCTGATCTTCAAGGAAACACCAATAATAACACGAATTCATCATCGGTGTCGCCTTTTAACCTAAGCTTCTTTCCGAATACCAATAGCAGCACAATGCATGATCAGTTCAACAACATAAGCGAACCTCATCATCATCATCATCATCATCATCATCACCAGCAGCATCACCAAGAAGGCTTTTCTTCACTCTTTGGTAATTCATCAATGCAACAAGAGAACATGTTGTCCTCACCGCACATGTCAGCAACTGCATTGCTTCAAAAAGCTGCTCAAATAGGTTCAACAACAACAACTACTATCAATAACAACGGTGTGGAATCTAATAATGAAGGATTAAGATCAAGCATGGAAAATGAACACAACCAACATCTACATGGATTGCTAAACT
TARGET=363,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5447
PRIMER_LEFT_PENALTY=0.274768
PRIMER_RIGHT_PENALTY=0.269968
PRIMER_LEFT_SEQUENCE=CACCAAGAAGGCTTTTCTTCAC
PRIMER_RIGHT_SEQUENCE=GATTCCACACCGTTGTTATTGA
PRIMER_LEFT=247,22
PRIMER_RIGHT=401,22
PRIMER_LEFT_TM=60.275
PRIMER_RIGHT_TM=59.730
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.6327
PRIMER_LEFT_1_PENALTY=0.362762
PRIMER_RIGHT_1_PENALTY=0.269968
PRIMER_LEFT_1_SEQUENCE=ACAACATAAGCGAACCTCATCA
PRIMER_RIGHT_1_SEQUENCE=GATTCCACACCGTTGTTATTGA
PRIMER_LEFT_1=191,22
PRIMER_RIGHT_1=401,22
PRIMER_LEFT_1_TM=59.637
PRIMER_RIGHT_1_TM=59.730
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.6728
PRIMER_LEFT_2_PENALTY=0.402858
PRIMER_RIGHT_2_PENALTY=0.269968
PRIMER_LEFT_2_SEQUENCE=ACATAAGCGAACCTCATCATCA
PRIMER_RIGHT_2_SEQUENCE=GATTCCACACCGTTGTTATTGA
PRIMER_LEFT_2=194,22
PRIMER_RIGHT_2=401,22
PRIMER_LEFT_2_TM=59.597
PRIMER_RIGHT_2_TM=59.730
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.7486
PRIMER_LEFT_3_PENALTY=0.478666
PRIMER_RIGHT_3_PENALTY=0.269968
PRIMER_LEFT_3_SEQUENCE=CCAAGAAGGCTTTTCTTCACTC
PRIMER_RIGHT_3_SEQUENCE=GATTCCACACCGTTGTTATTGA
PRIMER_LEFT_3=249,22
PRIMER_RIGHT_3=401,22
PRIMER_LEFT_3_TM=59.521
PRIMER_RIGHT_3_TM=59.730
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=153
PRIMER_PAIR_PENALTY_4=0.9385
PRIMER_LEFT_4_PENALTY=0.668546
PRIMER_RIGHT_4_PENALTY=0.269968
PRIMER_LEFT_4_SEQUENCE=TCAACAACATAAGCGAACCTCA
PRIMER_RIGHT_4_SEQUENCE=GATTCCACACCGTTGTTATTGA
PRIMER_LEFT_4=188,22
PRIMER_RIGHT_4=401,22
PRIMER_LEFT_4_TM=60.669
PRIMER_RIGHT_4_TM=59.730
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_05093 Ps_contig_mira-and-tgicl-ass_franssen_36432
SEQUENCE=TCGGGGATACTCTCATTGTCCCTCCNATTCAACCTTTGAAATTTTCCGAAATTTTCCGAAATTCAACACAGAGAAACCAAGTAAGAGAAAACTACCAAAACTTCCATGGCGTACACCAAGGAACGTGAAAACTTCGTCTACATCGCCAAGCTCGCTGAACAAGCCGAACGCTATGATGAAATGGTGGAAGCAATGAAGAATGTGGCAAAGCTAGATGTTGAGTTGACTGTGGAGGAGAGAAACCTTCTATCAGTTGGGTACAAGAACGTAGTGGGCGCTCATAGGGCTTCATGGAGGATTCTATCCTCGATTGAGCACAGGGAAGACACAAAAGGGAATGATGTGAGTGTGAAGAGGATAAGGGAGTATAGAAACAAGGTGGAATCTGAGTTGTCGAATATTTGCAGTGATATCATGACTATTATTGATGATCACCTTATTCCTTCATCCTCTGCTGGTGAGTCTAGTGTCTTTTTCTATAAAATGAAAGGGGATTATTATCGGTATTTGGCGGAGTTTAAGAACGGTGACGAGAGGAAAGAGGCTGCTGATCGTTCCATGGAAGCTTATCAGACAGCTTCTACTGCAGCCGAAGATGAGTTAGCTCCCACACATCCCATTCGATTGGGTTTGGCTTTGAACTTCTCTGTCTTTTATTATGAAATTTTGAACTCTCCTGAAAGGGCATGTCACCTTGCTAAGCAGGCATTTGATGAAGCAATCTCTGAGTTGGATACTCTGAGCGAGGAGTCTTACAAAGACAGCACATTGATTATGCAGCTTCTGAGGGACAACCTCACATTGTGGACTTCAGACATCCCTGAAGAAGGAGTTGAGGAACAAAAGGTTGAATCTTCGCGAGATGGTGGAGATGAAGATGCATAGGTCGGCACAATTGTCCAAACTCTTTGAACAAATTTCATGTGTTCTTTGTCTGCGGTTTTCGCAACTTTTTACCATTAGGATGTTGTAATAGGACAAGAAGTTGGGATTGCAGGGTGCTGTTTGACTGTAATTTAATGTTTGTTTGTTTTCAAGTACATAATTGCAAAAGGGATGCTTGCTTCATTTCATTCATCAATTTTCATATATTATACTTCTTACAGATAAGCCTTTTATTTGATCATGGATTGTGATTTCCATCCATATATAATTTTCGT
TARGET=1021,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9525
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.940300
PRIMER_LEFT_SEQUENCE=GGAGTTGAGGAACAAAAGGTTG
PRIMER_RIGHT_SEQUENCE=GAATGAAATGAAGCAAGCATCC
PRIMER_LEFT=828,22
PRIMER_RIGHT=1075,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.940
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.9601
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.940300
PRIMER_LEFT_1_SEQUENCE=GGAACAAAAGGTTGAATCTTCG
PRIMER_RIGHT_1_SEQUENCE=GAATGAAATGAAGCAAGCATCC
PRIMER_LEFT_1=836,22
PRIMER_RIGHT_1=1075,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=60.940
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=1.0361
PRIMER_LEFT_2_PENALTY=0.095771
PRIMER_RIGHT_2_PENALTY=0.940300
PRIMER_LEFT_2_SEQUENCE=TGGTGGAGATGAAGATGCATAG
PRIMER_RIGHT_2_SEQUENCE=GAATGAAATGAAGCAAGCATCC
PRIMER_LEFT_2=863,22
PRIMER_RIGHT_2=1075,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.940
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=213
PRIMER_PAIR_PENALTY_3=1.0504
PRIMER_LEFT_3_PENALTY=0.110131
PRIMER_RIGHT_3_PENALTY=0.940300
PRIMER_LEFT_3_SEQUENCE=ACATCCCTGAAGAAGGAGTTGA
PRIMER_RIGHT_3_SEQUENCE=GAATGAAATGAAGCAAGCATCC
PRIMER_LEFT_3=814,22
PRIMER_RIGHT_3=1075,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=60.940
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=1.0504
PRIMER_LEFT_4_PENALTY=0.110131
PRIMER_RIGHT_4_PENALTY=0.940300
PRIMER_LEFT_4_SEQUENCE=GACATCCCTGAAGAAGGAGTTG
PRIMER_RIGHT_4_SEQUENCE=GAATGAAATGAAGCAAGCATCC
PRIMER_LEFT_4=813,22
PRIMER_RIGHT_4=1075,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=60.940
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_05094 Ps_contig_mira-and-tgicl-ass_franssen_36437
SEQUENCE=CTATGACCATTGCTCATCCCTAAACTTTGTCGATCTTCTTCGCCTCTATCAATCTCACCACTCCCAGGATCCTCCTCCTCTTCTTCCTCCTCTTCATCCTCCTCCTCCTCATCGGACTCCGGCCTCTCATTCTCCTCCGCATCCATCTTATCCAAGAACTTAAGCGACTTAATCAAACCAAAAACCCTAGATCGATAGTCCTTAACACGAGTAACAGGACACTCATACAGATCCAACGAAACAAGCTTCAACTGCGCTAACGGAGCAAGATCCTCGATCGACTGAATCCTATTATTGGATAGATCGAGGTCACGCAGCGAATCGAGCCCCGCTTGAACCAAAAACTCAAGTCCTCCGGCGATCCGGTTGTCGGAGAGGTTCAACTTCTGAAGACTCCGGAGCCTCGGGAACTGCTCCAGAGAAGAAACNCCTAACG
TARGET=97,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2148
PRIMER_LEFT_PENALTY=0.043085
PRIMER_RIGHT_PENALTY=0.171757
PRIMER_LEFT_SEQUENCE=CCTCCTCTTCTTCCTCCTCTTC
PRIMER_RIGHT_SEQUENCE=AAGCTTGTTTCGTTGGATCTGT
PRIMER_LEFT=73,22
PRIMER_RIGHT=247,22
PRIMER_LEFT_TM=59.957
PRIMER_RIGHT_TM=60.172
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=0.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=175
PRIMER_PAIR_PENALTY_1=0.2148
PRIMER_LEFT_1_PENALTY=0.043085
PRIMER_RIGHT_1_PENALTY=0.171757
PRIMER_LEFT_1_SEQUENCE=TCCTCCTCTTCTTCCTCCTCTT
PRIMER_RIGHT_1_SEQUENCE=AAGCTTGTTTCGTTGGATCTGT
PRIMER_LEFT_1=72,22
PRIMER_RIGHT_1=247,22
PRIMER_LEFT_1_TM=59.957
PRIMER_RIGHT_1_TM=60.172
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=0.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=0.2734
PRIMER_LEFT_2_PENALTY=0.101680
PRIMER_RIGHT_2_PENALTY=0.171757
PRIMER_LEFT_2_SEQUENCE=AAACTTTGTCGATCTTCTTCGC
PRIMER_RIGHT_2_SEQUENCE=AAGCTTGTTTCGTTGGATCTGT
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=247,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=60.172
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=0.3790
PRIMER_LEFT_3_PENALTY=0.207235
PRIMER_RIGHT_3_PENALTY=0.171757
PRIMER_LEFT_3_SEQUENCE=GATCCTCCTCCTCTTCTTCCTC
PRIMER_RIGHT_3_SEQUENCE=AAGCTTGTTTCGTTGGATCTGT
PRIMER_LEFT_3=67,22
PRIMER_RIGHT_3=247,22
PRIMER_LEFT_3_TM=59.793
PRIMER_RIGHT_3_TM=60.172
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.3795
PRIMER_LEFT_4_PENALTY=0.043085
PRIMER_RIGHT_4_PENALTY=0.336392
PRIMER_LEFT_4_SEQUENCE=TCCTCCTCTTCTTCCTCCTCTT
PRIMER_RIGHT_4_SEQUENCE=TCGATCTAGGGTTTTTGGTTTG
PRIMER_LEFT_4=72,22
PRIMER_RIGHT_4=194,22
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=60.336
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=0.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_05095 Ps_contig_mira-and-tgicl-ass_franssen_36441
SEQUENCE=TATCAACGCAGAGTACGTCGGGGAGGGCTGGTTTGATTTAGGAGCGGCTGAAGAAAGAGTTAAGTCAGAGAGAGAGAGAAAGGTGTGAAGGAAAAATGAGCAACGAAGCAAAAGGCACGACGAAGATCGATGGTGGATTTAGGTCAAAATTGAATCACGCCCTCTACAGCGGTGAAAAGAAGCACGTTTTCGCTGGCTTAGTTCTCATCGCCGCCGTCTTTTCTGTACCATGGTTTCTTATGAATAGAGGATCAAAACATCAGTCTCATCAAGATTACATGGAAAAAGCTGATAAAGCAAGGAGCCAAAGACTCTCATCCAGACCTGCGTCTGCCAAATAATCTCGATTAGGGTTCTGTTAAAGATCACAGTTTCCAGTAACTCTAGATTTAAAAGCCATTAGATATGTTTGTTGGATGTCCTTTTCTCTCTACTTTTGTCATTGGGATGGATATCCATTTTTGTTCTATTTGCACTTCACTGCTAAATAGATCGGATTTTAATAATTAAAAAGACTATCATTGTTTGATATTTTGATGTCAGTAAATTCTCTATGTAATAAAATATGTTAGATTTCATTTTCCTGATTGAATCGTATAATAGGGCGATGAGACTTTTCATTTTATGTAGAGGCGGACATGCAACTTATTTCTCATATGAGTACTATGCTTCGGTGATGATTTTGCTGTAATGTTTGTAG
TARGET=67,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7681
PRIMER_LEFT_PENALTY=0.652901
PRIMER_RIGHT_PENALTY=0.115189
PRIMER_LEFT_SEQUENCE=AGCGGCTGAAGAAAGAGTTAAG
PRIMER_RIGHT_SEQUENCE=GTCTGGATGAGAGTCTTTGGCT
PRIMER_LEFT=42,22
PRIMER_RIGHT=323,22
PRIMER_LEFT_TM=59.347
PRIMER_RIGHT_TM=59.885
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.7681
PRIMER_LEFT_1_PENALTY=0.652901
PRIMER_RIGHT_1_PENALTY=0.115189
PRIMER_LEFT_1_SEQUENCE=AGCGGCTGAAGAAAGAGTTAAG
PRIMER_RIGHT_1_SEQUENCE=GCAGGTCTGGATGAGAGTCTTT
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=327,22
PRIMER_LEFT_1_TM=59.347
PRIMER_RIGHT_1_TM=59.885
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.8542
PRIMER_LEFT_2_PENALTY=0.652901
PRIMER_RIGHT_2_PENALTY=0.201288
PRIMER_LEFT_2_SEQUENCE=AGCGGCTGAAGAAAGAGTTAAG
PRIMER_RIGHT_2_SEQUENCE=CCTAAATCCACCATCGATCTTC
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=142,22
PRIMER_LEFT_2_TM=59.347
PRIMER_RIGHT_2_TM=59.799
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=101
PRIMER_PAIR_PENALTY_3=0.9136
PRIMER_LEFT_3_PENALTY=0.652901
PRIMER_RIGHT_3_PENALTY=0.260719
PRIMER_LEFT_3_SEQUENCE=AGCGGCTGAAGAAAGAGTTAAG
PRIMER_RIGHT_3_SEQUENCE=AGGTCTGGATGAGAGTCTTTGG
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=325,22
PRIMER_LEFT_3_TM=59.347
PRIMER_RIGHT_3_TM=59.739
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.9277
PRIMER_LEFT_4_PENALTY=0.652901
PRIMER_RIGHT_4_PENALTY=0.274768
PRIMER_LEFT_4_SEQUENCE=AGCGGCTGAAGAAAGAGTTAAG
PRIMER_RIGHT_4_SEQUENCE=AACCATGGTACAGAAAAGACGG
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=234,22
PRIMER_LEFT_4_TM=59.347
PRIMER_RIGHT_4_TM=60.275
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_05096 Ps_contig_mira-and-tgicl-ass_franssen_36444
SEQUENCE=CGCCTTGTTCCCCTCGTCAGGAGGATTCCCCCTTCGTTTTCCAAAGATTTCTTTGTTAACGATGATTCTGCGACTAAGACTCYTTGTCGCATTCTCAATCTTAACTTGGATGATGATGAATGATGCTCAAAGAAGAGCAGATGGTTACTGTAAATAAGTCTTTTTTTAATGAAGTTGGGTTAGTGTTAGTTTTGCTGTGTATATAAGGGTGGCCAAATAAAAGTATGACTTTGGTTGTTTGAGTTTGTGTAGCATTCAGTAGTGGTGTATGTAGTGCCCTTGGTAACAATGATTAATGGCTGTCGTTGGTTGACATTCTCTGTAATTTGGAAATCGGATCAGGTTTGAATGTGGAAAAAGGGTAAGTGGGTGTATGATGTATATGTAAAGCTCTTTTGGGAGCAGGTTTTGGTTTGTGACTTAGTTGACATTGATGGAAGCTGCAGGGAAAATGGTACGGAGGCAGAGATGACGGTAATAGGTGGGAGTGGCATGACGGAAAATAGTTTGGCCGGAAGAAAGTTACTTGTTATATCGATGAAATATATAGTTCACAGCATCATTTCTTCTCAGAATGATTAACTTAATTTAACCTGATTAAGGATTTAGACCACTTGCTTTAGTACATACTTTAGGTTGTATTGTATCTAATCTTGAACTTACAAAACTCGTTAGACATCAATCCTTTGGTCCTTAATCCTCTCTTAATACTTCATTTCTTTTCGTTTCTTATCACATTATATATATACTCCTCTAGCCTTGTAGGTGACTTACTGTTTACGAGTTAGTAGATAGTTACTACCTTTCTTTCAGATCCTTCTCTCCTAGTTATCTTTTGTTTCTCCCTCTATGAGGGCAAAGTTTGTCCCTTCGCCATCTTTTCTGTTTTTTACCAAGAAAAGCTGGAAGGGTTTGGTTTTATATAGTCTTTTGTAGCTGAATGCTTTAGCTTCTGTAGGTTAAATCCTTGTCACACTGTATGTTACTCACCATTCATCTAAACCCGTATATGTCCCATTTACGCCTTTTGGTCATTGCTTCTTTCTATTGTCTTCACCTGTCTTATCCTCCATGGAGATTGTTGGCTATACTACGCCGAACTTAAGGGAGGTGTTACTGCACTGATGCTGTCATGTTCTACATGGCTTGTTCAACTTCAAGTTTGTATCTGTTCGTGAAGTCCCCCATAGTTCGTAAACTTTGCAGTGTTTTGTTCTTTTGTGCCTTTGTAACTTGTGGAAATTGCTTGATCTGTTGAGATAATTGCGTAACAACTATGATCTACTTTTCATGTTTTATGTTCGAGTTAAGAACTAAGTACTTGTATTCTCTTGTCTTAGTTATAAGGTTGCTTTGAAAGTTCTGATACTATTTATGTTTTTAATATAATGTGGACGAATTTGCTTTGTTCTTAAACTGAAATTTGGTTGTCTCGTATGTTTTAATCGATGGTCTAATATTTTCTATTAAT
TARGET=739,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05097 Ps_contig_mira-and-tgicl-ass_franssen_36451
SEQUENCE=AATTAATACATACTAGCTACCATCCCAACTACTNNNNGAAAGGTTCCATATTGATTCTTACTTCCATCCATTATATATAGAGCACACTTTCTTACTATTTCGCGGGATCCTTCACTACCAAATATCACATTTTGTTCGATCTTGAGTTGAGTACAATGGATTCCAAAAATGCAATCCTCATCCTTGGCATATTGGCCATGGTTCTTCTTATCTCCTCACAGGTTTCAGCTAGGGACTTAGCTGACACTTCCACTAATACCAAAGATGTGGTTGTTGAAAAATCACATGAATTAAATGATGCCAAATATGGTGGTAGAGGCTACGGTCCAGGTGGTGGTTACAATGGAGGTGGAGGTTATAATGGTGGTGGTGGTGGTTACAATGGTGGTGGTGGTTATCATAATGGCGGAGGTGGTTACCATGGTGGTGGTGGTGGTTACAATGGTGGTGGTGGTTACCATGGTGGAGGTAGATGCCGATACGGCTGTTGTGGTGGTGGACGTTACGGGGGTTGCAGGTGTTGCTCATATGCTGGTGAAGCTGTTGCTGTGCACACTGAGGACAACACTCGTAACTGAATATTATCATGTCATCATGCATGATGCACTTTCTTAGAAATAAATTTTATGAATAAATAATGATGTGTGTTGAGAATAAGAGGTATAGATAGACAGCAATTTGTGTTGTTGTGTGTTATATATCTTGCAACTATATAGTATAGATATTTCACATCTGTGAAAATCATGTATAACTACATCGTTACTATGTATATGTGATGATGAGTTAAAGTTTTAAGTTTTAACAAAAATGGTGAAAGTGTTATGAGTTAAATTATTTCAAATATTATGGTTGTTGATACTTTCCCTCT
TARGET=362,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5174
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.483253
PRIMER_LEFT_SEQUENCE=TTGGCCATGGTTCTTCTTATCT
PRIMER_RIGHT_SEQUENCE=TAACCACCACCACCATTGTAAC
PRIMER_LEFT=191,22
PRIMER_RIGHT=456,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.517
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.5174
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.483253
PRIMER_LEFT_1_SEQUENCE=TTGGCCATGGTTCTTCTTATCT
PRIMER_RIGHT_1_SEQUENCE=GTAACCACCACCACCATTGTAA
PRIMER_LEFT_1=191,22
PRIMER_RIGHT_1=457,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.517
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.5584
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.524309
PRIMER_LEFT_2_SEQUENCE=TTGGCCATGGTTCTTCTTATCT
PRIMER_RIGHT_2_SEQUENCE=GATAACCACCACCACCATTGTA
PRIMER_LEFT_2=191,22
PRIMER_RIGHT_2=398,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=59.476
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.6540
PRIMER_LEFT_3_PENALTY=0.170703
PRIMER_RIGHT_3_PENALTY=0.483253
PRIMER_LEFT_3_SEQUENCE=AATCCTCATCCTTGGCATATTG
PRIMER_RIGHT_3_SEQUENCE=GTAACCACCACCACCATTGTAA
PRIMER_LEFT_3=172,22
PRIMER_RIGHT_3=457,22
PRIMER_LEFT_3_TM=60.171
PRIMER_RIGHT_3_TM=59.517
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=0.6540
PRIMER_LEFT_4_PENALTY=0.170703
PRIMER_RIGHT_4_PENALTY=0.483253
PRIMER_LEFT_4_SEQUENCE=AATCCTCATCCTTGGCATATTG
PRIMER_RIGHT_4_SEQUENCE=TAACCACCACCACCATTGTAAC
PRIMER_LEFT_4=172,22
PRIMER_RIGHT_4=456,22
PRIMER_LEFT_4_TM=60.171
PRIMER_RIGHT_4_TM=59.517
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_05098 Ps_contig_mira-and-tgicl-ass_franssen_36451
SEQUENCE=AATTAATACATACTAGCTACCATCCCAACTACTNNNNGAAAGGTTCCATATTGATTCTTACTTCCATCCATTATATATAGAGCACACTTTCTTACTATTTCGCGGGATCCTTCACTACCAAATATCACATTTTGTTCGATCTTGAGTTGAGTACAATGGATTCCAAAAATGCAATCCTCATCCTTGGCATATTGGCCATGGTTCTTCTTATCTCCTCACAGGTTTCAGCTAGGGACTTAGCTGACACTTCCACTAATACCAAAGATGTGGTTGTTGAAAAATCACATGAATTAAATGATGCCAAATATGGTGGTAGAGGCTACGGTCCAGGTGGTGGTTACAATGGAGGTGGAGGTTATAATGGTGGTGGTGGTGGTTACAATGGTGGTGGTGGTTATCATAATGGCGGAGGTGGTTACCATGGTGGTGGTGGTGGTTACAATGGTGGTGGTGGTTACCATGGTGGAGGTAGATGCCGATACGGCTGTTGTGGTGGTGGACGTTACGGGGGTTGCAGGTGTTGCTCATATGCTGGTGAAGCTGTTGCTGTGCACACTGAGGACAACACTCGTAACTGAATATTATCATGTCATCATGCATGATGCACTTTCTTAGAAATAAATTTTATGAATAAATAATGATGTGTGTTGAGAATAAGAGGTATAGATAGACAGCAATTTGTGTTGTTGTGTGTTATATATCTTGCAACTATATAGTATAGATATTTCACATCTGTGAAAATCATGTATAACTACATCGTTACTATGTATATGTGATGATGAGTTAAAGTTTTAAGTTTTAACAAAAATGGTGAAAGTGTTATGAGTTAAATTATTTCAAATATTATGGTTGTTGATACTTTCCCTCT
TARGET=383,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5174
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.483253
PRIMER_LEFT_SEQUENCE=TTGGCCATGGTTCTTCTTATCT
PRIMER_RIGHT_SEQUENCE=TAACCACCACCACCATTGTAAC
PRIMER_LEFT=191,22
PRIMER_RIGHT=456,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.517
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.5174
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.483253
PRIMER_LEFT_1_SEQUENCE=TTGGCCATGGTTCTTCTTATCT
PRIMER_RIGHT_1_SEQUENCE=GTAACCACCACCACCATTGTAA
PRIMER_LEFT_1=191,22
PRIMER_RIGHT_1=457,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=59.517
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.6540
PRIMER_LEFT_2_PENALTY=0.170703
PRIMER_RIGHT_2_PENALTY=0.483253
PRIMER_LEFT_2_SEQUENCE=AATCCTCATCCTTGGCATATTG
PRIMER_RIGHT_2_SEQUENCE=GTAACCACCACCACCATTGTAA
PRIMER_LEFT_2=172,22
PRIMER_RIGHT_2=457,22
PRIMER_LEFT_2_TM=60.171
PRIMER_RIGHT_2_TM=59.517
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=0.6540
PRIMER_LEFT_3_PENALTY=0.170703
PRIMER_RIGHT_3_PENALTY=0.483253
PRIMER_LEFT_3_SEQUENCE=AATCCTCATCCTTGGCATATTG
PRIMER_RIGHT_3_SEQUENCE=TAACCACCACCACCATTGTAAC
PRIMER_LEFT_3=172,22
PRIMER_RIGHT_3=456,22
PRIMER_LEFT_3_TM=60.171
PRIMER_RIGHT_3_TM=59.517
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.6540
PRIMER_LEFT_4_PENALTY=0.170703
PRIMER_RIGHT_4_PENALTY=0.483253
PRIMER_LEFT_4_SEQUENCE=CAATCCTCATCCTTGGCATATT
PRIMER_RIGHT_4_SEQUENCE=TAACCACCACCACCATTGTAAC
PRIMER_LEFT_4=171,22
PRIMER_RIGHT_4=456,22
PRIMER_LEFT_4_TM=60.171
PRIMER_RIGHT_4_TM=59.517
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_05099 Ps_contig_mira-and-tgicl-ass_franssen_36451
SEQUENCE=AATTAATACATACTAGCTACCATCCCAACTACTNNNNGAAAGGTTCCATATTGATTCTTACTTCCATCCATTATATATAGAGCACACTTTCTTACTATTTCGCGGGATCCTTCACTACCAAATATCACATTTTGTTCGATCTTGAGTTGAGTACAATGGATTCCAAAAATGCAATCCTCATCCTTGGCATATTGGCCATGGTTCTTCTTATCTCCTCACAGGTTTCAGCTAGGGACTTAGCTGACACTTCCACTAATACCAAAGATGTGGTTGTTGAAAAATCACATGAATTAAATGATGCCAAATATGGTGGTAGAGGCTACGGTCCAGGTGGTGGTTACAATGGAGGTGGAGGTTATAATGGTGGTGGTGGTGGTTACAATGGTGGTGGTGGTTATCATAATGGCGGAGGTGGTTACCATGGTGGTGGTGGTGGTTACAATGGTGGTGGTGGTTACCATGGTGGAGGTAGATGCCGATACGGCTGTTGTGGTGGTGGACGTTACGGGGGTTGCAGGTGTTGCTCATATGCTGGTGAAGCTGTTGCTGTGCACACTGAGGACAACACTCGTAACTGAATATTATCATGTCATCATGCATGATGCACTTTCTTAGAAATAAATTTTATGAATAAATAATGATGTGTGTTGAGAATAAGAGGTATAGATAGACAGCAATTTGTGTTGTTGTGTGTTATATATCTTGCAACTATATAGTATAGATATTTCACATCTGTGAAAATCATGTATAACTACATCGTTACTATGTATATGTGATGATGAGTTAAAGTTTTAAGTTTTAACAAAAATGGTGAAAGTGTTATGAGTTAAATTATTTCAAATATTATGGTTGTTGATACTTTCCCTCT
TARGET=422,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0010
PRIMER_LEFT_PENALTY=0.406861
PRIMER_RIGHT_PENALTY=0.594102
PRIMER_LEFT_SEQUENCE=TGGAGGTGGAGGTTATAATGGT
PRIMER_RIGHT_SEQUENCE=AACAGCTTCACCAGCATATGAG
PRIMER_LEFT=343,22
PRIMER_RIGHT=544,22
PRIMER_LEFT_TM=59.593
PRIMER_RIGHT_TM=59.406
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=1.0010
PRIMER_LEFT_1_PENALTY=0.406861
PRIMER_RIGHT_1_PENALTY=0.594102
PRIMER_LEFT_1_SEQUENCE=GGAGGTGGAGGTTATAATGGTG
PRIMER_RIGHT_1_SEQUENCE=AACAGCTTCACCAGCATATGAG
PRIMER_LEFT_1=344,22
PRIMER_RIGHT_1=544,22
PRIMER_LEFT_1_TM=59.593
PRIMER_RIGHT_1_TM=59.406
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=1.0774
PRIMER_LEFT_2_PENALTY=0.483253
PRIMER_RIGHT_2_PENALTY=0.594102
PRIMER_LEFT_2_SEQUENCE=GTTACAATGGTGGTGGTGGTTA
PRIMER_RIGHT_2_SEQUENCE=AACAGCTTCACCAGCATATGAG
PRIMER_LEFT_2=375,22
PRIMER_RIGHT_2=544,22
PRIMER_LEFT_2_TM=59.517
PRIMER_RIGHT_2_TM=59.406
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=1.1184
PRIMER_LEFT_3_PENALTY=0.524309
PRIMER_RIGHT_3_PENALTY=0.594102
PRIMER_LEFT_3_SEQUENCE=TACAATGGTGGTGGTGGTTATC
PRIMER_RIGHT_3_SEQUENCE=AACAGCTTCACCAGCATATGAG
PRIMER_LEFT_3=377,22
PRIMER_RIGHT_3=544,22
PRIMER_LEFT_3_TM=59.476
PRIMER_RIGHT_3_TM=59.406
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=1.1865
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=1.091031
PRIMER_LEFT_4_SEQUENCE=ATGCCAAATATGGTGGTAGAGG
PRIMER_RIGHT_4_SEQUENCE=TTCACCAGCATATGAGCAACAC
PRIMER_LEFT_4=297,22
PRIMER_RIGHT_4=538,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=61.091
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_05100 Ps_contig_mira-and-tgicl-ass_franssen_36451
SEQUENCE=AATTAATACATACTAGCTACCATCCCAACTACTNNNNGAAAGGTTCCATATTGATTCTTACTTCCATCCATTATATATAGAGCACACTTTCTTACTATTTCGCGGGATCCTTCACTACCAAATATCACATTTTGTTCGATCTTGAGTTGAGTACAATGGATTCCAAAAATGCAATCCTCATCCTTGGCATATTGGCCATGGTTCTTCTTATCTCCTCACAGGTTTCAGCTAGGGACTTAGCTGACACTTCCACTAATACCAAAGATGTGGTTGTTGAAAAATCACATGAATTAAATGATGCCAAATATGGTGGTAGAGGCTACGGTCCAGGTGGTGGTTACAATGGAGGTGGAGGTTATAATGGTGGTGGTGGTGGTTACAATGGTGGTGGTGGTTATCATAATGGCGGAGGTGGTTACCATGGTGGTGGTGGTGGTTACAATGGTGGTGGTGGTTACCATGGTGGAGGTAGATGCCGATACGGCTGTTGTGGTGGTGGACGTTACGGGGGTTGCAGGTGTTGCTCATATGCTGGTGAAGCTGTTGCTGTGCACACTGAGGACAACACTCGTAACTGAATATTATCATGTCATCATGCATGATGCACTTTCTTAGAAATAAATTTTATGAATAAATAATGATGTGTGTTGAGAATAAGAGGTATAGATAGACAGCAATTTGTGTTGTTGTGTGTTATATATCTTGCAACTATATAGTATAGATATTTCACATCTGTGAAAATCATGTATAACTACATCGTTACTATGTATATGTGATGATGAGTTAAAGTTTTAAGTTTTAACAAAAATGGTGAAAGTGTTATGAGTTAAATTATTTCAAATATTATGGTTGTTGATACTTTCCCTCT
TARGET=443,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0010
PRIMER_LEFT_PENALTY=0.406861
PRIMER_RIGHT_PENALTY=0.594102
PRIMER_LEFT_SEQUENCE=TGGAGGTGGAGGTTATAATGGT
PRIMER_RIGHT_SEQUENCE=AACAGCTTCACCAGCATATGAG
PRIMER_LEFT=343,22
PRIMER_RIGHT=544,22
PRIMER_LEFT_TM=59.593
PRIMER_RIGHT_TM=59.406
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=1.0010
PRIMER_LEFT_1_PENALTY=0.406861
PRIMER_RIGHT_1_PENALTY=0.594102
PRIMER_LEFT_1_SEQUENCE=GGAGGTGGAGGTTATAATGGTG
PRIMER_RIGHT_1_SEQUENCE=AACAGCTTCACCAGCATATGAG
PRIMER_LEFT_1=344,22
PRIMER_RIGHT_1=544,22
PRIMER_LEFT_1_TM=59.593
PRIMER_RIGHT_1_TM=59.406
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=1.0774
PRIMER_LEFT_2_PENALTY=0.483253
PRIMER_RIGHT_2_PENALTY=0.594102
PRIMER_LEFT_2_SEQUENCE=GTTACAATGGTGGTGGTGGTTA
PRIMER_RIGHT_2_SEQUENCE=AACAGCTTCACCAGCATATGAG
PRIMER_LEFT_2=375,22
PRIMER_RIGHT_2=544,22
PRIMER_LEFT_2_TM=59.517
PRIMER_RIGHT_2_TM=59.406
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=1.1184
PRIMER_LEFT_3_PENALTY=0.524309
PRIMER_RIGHT_3_PENALTY=0.594102
PRIMER_LEFT_3_SEQUENCE=TACAATGGTGGTGGTGGTTATC
PRIMER_RIGHT_3_SEQUENCE=AACAGCTTCACCAGCATATGAG
PRIMER_LEFT_3=377,22
PRIMER_RIGHT_3=544,22
PRIMER_LEFT_3_TM=59.476
PRIMER_RIGHT_3_TM=59.406
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=1.1865
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=1.091031
PRIMER_LEFT_4_SEQUENCE=ATGCCAAATATGGTGGTAGAGG
PRIMER_RIGHT_4_SEQUENCE=TTCACCAGCATATGAGCAACAC
PRIMER_LEFT_4=297,22
PRIMER_RIGHT_4=538,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=61.091
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_05101 Ps_contig_mira-and-tgicl-ass_franssen_36453
SEQUENCE=CTCCTACACTAAACTCACGTCCTATACATTCTTATCTTCAAAACAATTCTAGGANATGATCAAACAACTTCCTCCTCTAACATGGCCACCATAGCTCAGGTAGTAGAACAAGACCATGCTCCTCCTCCTCACCATGCCATGTTTCTGGTCATACCATACCTTCGAGTGTACGAGGTTTTAACCATGTCTCTTGTTTGCACATCACTAAGGGATGCAGTTAACAATGATGTATTGCCATGGCTTCGTATTATTATCGAAACGCCTCTCAATTATCGACTCACCAACGAGACTCTGTTGAAAATCACATCCAAATCCAATGGTAGGCTTCAAAAACTTGCTCTCATGAACTGTATCCACATCACTAATCAAGGACTACAAAGGGTCATTGAACAAAACCCTTTCATTAAAGAGCTTCATATACCAGCATGCACTAGCATAACCCCTGAAGGTGTTCTAAAAGCTGTAGAAACATTGTGCCAAAGAAACAATTGCTTAACCACCTTGAGTATAAATGGAATCTACAATCTACAGAAGGAGCATCTTGACATCCTTACTTCAAAGCTTAGAGAAAACCTTTCATTAAAAAACATGCAAATGCAGCAACCTATCTACTATCATAATCGCGGCAGCGTTTCAGCGTTCGAGTGTCGAGAAAATCATCGGATTATCGATTTGGAGATATGTCCTAAGTGCACTGAAGTGAAGATGGTATATGACTGCCCAAAAGTGGACTGCAAGATGAAGGAATGTAGAGGGTGCGTATTTTGTATTTCGAGGTGTGAAAACTGTGGTGGATGTGTTGGATCAGAAGAACCTGAAGAGTGTGCTTGTGGAGATATCATGTGCCTTGAATGTTGGTTAAAGGTTCCTAAATGCAGTTGCTGTAACAAACCATATTGTAAGCAGCACACAAATTGGTGGTGTACTTCTACAGACTCTTCATTAATGTGTAGAGTTTGTGATGAAAATTCTCATGGATATACATACACTGATGAGCTATAATGAAAAACTGACTCATGATGATGAACCTTACTATATCATCATGCCAAGTTATTTTCAGTTACTGTAATTTGTTTCAAGTTTACGGCTAAAGAAAATTATTGTTGATCATTGTGTTTACGTTACATGTAATTTTACTTTGTCATTGACTGAGGTTATTATATANGAAGTA
TARGET=119,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2320
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.101680
PRIMER_LEFT_SEQUENCE=CAACTTCCTCCTCTAACATGGC
PRIMER_RIGHT_SEQUENCE=ATGAGAGCAAGTTTTTGAAGCC
PRIMER_LEFT=64,22
PRIMER_RIGHT=343,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.2330
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.102638
PRIMER_LEFT_1_SEQUENCE=CAACTTCCTCCTCTAACATGGC
PRIMER_RIGHT_1_SEQUENCE=GGTGAGTCGATAATTGAGAGGC
PRIMER_LEFT_1=64,22
PRIMER_RIGHT_1=281,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.103
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=218
PRIMER_PAIR_PENALTY_2=0.3781
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.247716
PRIMER_LEFT_2_SEQUENCE=CAACTTCCTCCTCTAACATGGC
PRIMER_RIGHT_2_SEQUENCE=GAGTCGATAATTGAGAGGCGTT
PRIMER_LEFT_2=64,22
PRIMER_RIGHT_2=278,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=59.752
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=0.4188
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.288468
PRIMER_LEFT_3_SEQUENCE=CAACTTCCTCCTCTAACATGGC
PRIMER_RIGHT_3_SEQUENCE=AGCAAGTTTTTGAAGCCTACCA
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=338,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=60.288
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=0.4453
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.314970
PRIMER_LEFT_4_SEQUENCE=CAACTTCCTCCTCTAACATGGC
PRIMER_RIGHT_4_SEQUENCE=CTACCATTGGATTTGGATGTGA
PRIMER_LEFT_4=64,22
PRIMER_RIGHT_4=322,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=59.685
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=259
=
PRIMER_SEQUENCE_ID=SSR_05102 Ps_contig_mira-and-tgicl-ass_franssen_36455
SEQUENCE=CTTAATGTACTTTGATTGTTGTTTTGTGTCCTTGTAGCAAGCAAATGAGATGGTAGATGTGTGTGTGGATGACAAGGTTACAAACTACTTAAACAGAAGAGATGTGCAAGAGGCACTTCATGCAAAGCTCGTCGGAGTTCGCAAGTGGGATGTCTGCAGCAATGTTTTGGACTATGATGTGCTTAACTTGGAAGTGCCAACACTTCCTATTCTTGGATCACTGATAAGAGCTGGAATTAAGATCCTAATTTACAGTGGAGATCAAGACTCAGTAATTCCACTGACTGGAAGCAGAACCTTAGTTCAAAAATTGGCCAGACAAATAGGACTGAATACAACAGTCCCTTACAGAGTCTGGTTTGAAAGACAACAGGTTGGTGGATGGACTCAAGTGTATGGAAATATTCTGTCATTTGCCACAGTGAGAGGTGCTGCACATGAAAGCTCCTTTCTCACAGCCAGAAAGATCACTCGTGCTATTCAAATCATTCTTGGAAGGAACTCCTTTGCCTGAAGTTTTCTGACACAGCTATTTTCCCCTTTTATTAGTTAAAGGTTGTATTGTAAATAAG
TARGET=58,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0205
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=0.002623
PRIMER_LEFT_SEQUENCE=TCCTTGTAGCAAGCAAATGAGA
PRIMER_RIGHT_SEQUENCE=CTGGCCAATTTTTGAACTAAGG
PRIMER_LEFT=28,22
PRIMER_RIGHT=317,22
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=59.997
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=290
PRIMER_PAIR_PENALTY_1=0.1560
PRIMER_LEFT_1_PENALTY=0.017846
PRIMER_RIGHT_1_PENALTY=0.138114
PRIMER_LEFT_1_SEQUENCE=TCCTTGTAGCAAGCAAATGAGA
PRIMER_RIGHT_1_SEQUENCE=TCATAGTCCAAAACATTGCTGC
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=176,22
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=60.138
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.1815
PRIMER_LEFT_2_PENALTY=0.017846
PRIMER_RIGHT_2_PENALTY=0.163658
PRIMER_LEFT_2_SEQUENCE=TCCTTGTAGCAAGCAAATGAGA
PRIMER_RIGHT_2_SEQUENCE=TCCAGCTCTTATCAGTGATCCA
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=234,22
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=59.836
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.2280
PRIMER_LEFT_3_PENALTY=0.225403
PRIMER_RIGHT_3_PENALTY=0.002623
PRIMER_LEFT_3_SEQUENCE=TGTAGCAAGCAAATGAGATGGT
PRIMER_RIGHT_3_SEQUENCE=CTGGCCAATTTTTGAACTAAGG
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=317,22
PRIMER_LEFT_3_TM=59.775
PRIMER_RIGHT_3_TM=59.997
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=0.2669
PRIMER_LEFT_4_PENALTY=0.264300
PRIMER_RIGHT_4_PENALTY=0.002623
PRIMER_LEFT_4_SEQUENCE=TTGTAGCAAGCAAATGAGATGG
PRIMER_RIGHT_4_SEQUENCE=CTGGCCAATTTTTGAACTAAGG
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=317,22
PRIMER_LEFT_4_TM=60.264
PRIMER_RIGHT_4_TM=59.997
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_05103 Ps_contig_mira-and-tgicl-ass_franssen_36458
SEQUENCE=TTTCTTCTTACCACCTCTTTAGTTTCAGAATTCTAATCACAGGTACTTTTGGCTTCTGCAGCTGAAGATTCTACTACAGCTTCTTCTTCTTTTTCTTCATTGGTTTCTGATGCTGCTGGTGCAATTGGACTCTCAAGAACTTCTTCAGTGGTTTTCTCATCAGATTTCAATAATAACCCGGTGGTGTCGTCCGAGAGTGCAGTCTTTGCAGATACAACCTCAACAGTGGCCATAATAGGAATAGAAAGAAAATGCTTAAAGAGAAACTTAGTTAAAGAGTTGTATAGGAAACTATGCCTTTAATTTTTGATCAATAACCCCG
TARGET=80,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9182
PRIMER_LEFT_PENALTY=0.580184
PRIMER_RIGHT_PENALTY=0.338015
PRIMER_LEFT_SEQUENCE=TCACAGGTACTTTTGGCTTCTG
PRIMER_RIGHT_SEQUENCE=GCCACTGTTGAGGTTGTATCTG
PRIMER_LEFT=36,22
PRIMER_RIGHT=229,22
PRIMER_LEFT_TM=59.420
PRIMER_RIGHT_TM=59.662
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=0.9182
PRIMER_LEFT_1_PENALTY=0.580184
PRIMER_RIGHT_1_PENALTY=0.338015
PRIMER_LEFT_1_SEQUENCE=TCACAGGTACTTTTGGCTTCTG
PRIMER_RIGHT_1_SEQUENCE=CCACTGTTGAGGTTGTATCTGC
PRIMER_LEFT_1=36,22
PRIMER_RIGHT_1=228,22
PRIMER_LEFT_1_TM=59.420
PRIMER_RIGHT_1_TM=59.662
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.9242
PRIMER_LEFT_2_PENALTY=0.580184
PRIMER_RIGHT_2_PENALTY=0.343977
PRIMER_LEFT_2_SEQUENCE=TCACAGGTACTTTTGGCTTCTG
PRIMER_RIGHT_2_SEQUENCE=ACACCACCGGGTTATTATTGAA
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=186,22
PRIMER_LEFT_2_TM=59.420
PRIMER_RIGHT_2_TM=60.344
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=151
PRIMER_PAIR_PENALTY_3=0.9999
PRIMER_LEFT_3_PENALTY=0.580184
PRIMER_RIGHT_3_PENALTY=0.419726
PRIMER_LEFT_3_SEQUENCE=TCACAGGTACTTTTGGCTTCTG
PRIMER_RIGHT_3_SEQUENCE=TATGGCCACTGTTGAGGTTGTA
PRIMER_LEFT_3=36,22
PRIMER_RIGHT_3=233,22
PRIMER_LEFT_3_TM=59.420
PRIMER_RIGHT_3_TM=60.420
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=1.0038
PRIMER_LEFT_4_PENALTY=0.580184
PRIMER_RIGHT_4_PENALTY=0.423592
PRIMER_LEFT_4_SEQUENCE=TCACAGGTACTTTTGGCTTCTG
PRIMER_RIGHT_4_SEQUENCE=GGCCACTGTTGAGGTTGTATCT
PRIMER_LEFT_4=36,22
PRIMER_RIGHT_4=230,22
PRIMER_LEFT_4_TM=59.420
PRIMER_RIGHT_4_TM=60.424
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_05104 Ps_contig_mira-and-tgicl-ass_franssen_36460
SEQUENCE=ACAAATTCCCCAAAACAATTTAAGATTGCAATTCCCAAAATAGAAGTTTATTTTACGGGAACAGGAGACCTCATGACTTCACTTCTTCTCGGTTGGAGCAATAAACACCCGGACAATCTTGAGAAGGCTGCAAAACTTGCAGTTTCAAGCTTACAGGCACTTTTGCAAAGGACACTTACTGATTACAAAAGTGCTGGACATGACCCCAAATCATCCAGTTTAGAGATCAGGTTAATTCAGAGCCAGGATGATATTCGCAGTCCGGAAATCAAATTCAAAGCTGAAATATACAGCTAACTGGTGATTGGTGATATGGTTTGTAGCTGAATGTCTGTTTATTGATTGATTATAGAAATTGGAGCTACACTGTTGAATTTAGCACTGCAGGCCTATTGAGCATAACATGAGTCAAATTTGTTATGTCATAAATAAAATAAAATAAAGCTTATAGGCTCTAAATTTAATTTTAATATACTAGGCCTTGTGTATGTATATGTAATATTTAAGGACTTGTGATATATATATATTTTTTAGNCATT
TARGET=427,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05105 Ps_contig_mira-and-tgicl-ass_franssen_36460
SEQUENCE=ACAAATTCCCCAAAACAATTTAAGATTGCAATTCCCAAAATAGAAGTTTATTTTACGGGAACAGGAGACCTCATGACTTCACTTCTTCTCGGTTGGAGCAATAAACACCCGGACAATCTTGAGAAGGCTGCAAAACTTGCAGTTTCAAGCTTACAGGCACTTTTGCAAAGGACACTTACTGATTACAAAAGTGCTGGACATGACCCCAAATCATCCAGTTTAGAGATCAGGTTAATTCAGAGCCAGGATGATATTCGCAGTCCGGAAATCAAATTCAAAGCTGAAATATACAGCTAACTGGTGATTGGTGATATGGTTTGTAGCTGAATGTCTGTTTATTGATTGATTATAGAAATTGGAGCTACACTGTTGAATTTAGCACTGCAGGCCTATTGAGCATAACATGAGTCAAATTTGTTATGTCATAAATAAAATAAAATAAAGCTTATAGGCTCTAAATTTAATTTTAATATACTAGGCCTTGTGTATGTATATGTAATATTTAAGGACTTGTGATATATATATATTTTTTAGNCATT
TARGET=516,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05106 Ps_contig_mira-and-tgicl-ass_franssen_36464
SEQUENCE=CTACCTCCTGTGNGTGTCATATTATATGGAAAAATAACAACAAAAAAGTGCAATATGAAAAAAGGTAGTCAACGYAGAGTTGAGAAATTGGGAAGGGTTTTTGTGATGTGCATCCTCATCATCATCATCACTTTACTATCTTTTTTGAGAAAACTTTGCTAAAGCATTTTTGGTGGCTGATTAAGACCATTTAGTGTGGACAGCAGGTGCAGGTGCAGATAATAATCTGAGTTTTTAGGTTAATGTAGAGTTAGTTAACATTTTGTTTTTTTGCTAATTAGTTACTAGTTCTCAATCCACTTCACTATTTTAGTTGCTAAGTT
TARGET=116,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05107 Ps_contig_mira-and-tgicl-ass_franssen_36471
SEQUENCE=GTCGGGGGCCGCTACCGCTTGCCCTTCTCTACCATCATTCTCAAGCAAGCTGTAGTCGCACTCAACCCCCCGGAGTTAACCGTCGTAAGAACCAACATCACCACAAACAACAAGAATTCAATTGACCTAAAATGGGAGTTGGACGTGAAGTCTCAATCTCACTCGATGGAGTAAGAGACAAGAACATTATGCAGCTCAAGAAGCTCAATATTGCTCTCTTCCCCGTTCGTTACAACGATAAATACTACTCCGATGCTCTTGCTTCCGCCGAATTCACCAAACTAGCTTACTACAGTGACATTTGTGTTGGTGCGATTGCGTGCCGGCTTGAGAAGAAGGAAGGTGGAGGCCAAGTTCGGGTTTATATCATGACTTTAGGTGTTTTAGCACCTTACCGTGGACTTGGTATTGGAACGAAGCTGCTGAATCATGTTCTTGATCTTTGCTCCAAGCAGAACATTTCCGAGGTTTACTTGCACGTGCAGACAAACAACGAAGAAGCGATTAGTTTTTATAAGAAATTTGAGTTTGAAATTACAGAAACTATCCAGAACTATTACACAAACATCACACCACCAGATTGCTATGTCGTCACAAGGCACATAACTCCTAGCCCAACAAAGAAATAACATCTCATGTCAGTTATTTTGGGGCAATTCATCAAAGATTGTTGCATTATTTATTGAATTTTGCCATGAATGTATTACCCTGAAGTTTGCCGTTTTTAATGCTAGATTTTAAACAGCAAAGTTAGCCCAAAAATAGAAGCAATTGGGAATCTCTTTGCTAAGGTATTAAAGTTATTCTATCCCTATCTTCCCCCTTCCACACCCTATCTTACAATTTTAGGCAAAATATTTTTGAATTTGGTTGGTACAAGAGTCTTTTGGGGGAAATTGCACCTTGTTATTTTATTTTATAACTAAATAAATAAATATAGTCTTTTGCGTCA
TARGET=924,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05108 Ps_contig_mira-and-tgicl-ass_franssen_36472
SEQUENCE=AGTACGACTGTAAATTAAGTTATTAGTTATAGCGATTAGCGTAGTTGAAAGAGGAGCACAACAACACAAACAAAAAAACAGTTGGAAGAGAAGAGAAGAGAAGAGAAGATTAATCGATTCTCGATCGGAATAGAGAAAAACCCTAACGCAATCAATCAAATAGATGATTCAGAATTAGCAAAAATAGGTGTGTGCTTGTCGAAATCGAAGGGGCTTGCAAGAGCCTCTTCATCGGAGGTGGTGGTTTATTCCGAACCTCCACCTCCGACGGAATCCAAGAGCCTCTTCAAGCCTAAGCTCCGCACTCCTGCCGACATTGTCCGGAGCACTCGGGATCTTCTCCGTCTTCTCCAGAATGCTTCCGTTAACAAGCGCGATAACGACAAGATGATCGAGTTGTTTAAAAATTTTAAGAGAGATGAAAACAATTCTGTATGGAAATAGTGAAGCTGAACCAGTGCCGGAAGCTTGTTCGCAATTGACGCAGGAGTTCTTTAACGATAATACTTTTCGACTTCTGGTTGAGTGTCTTCCCAAATTGAACTTGGAGGCTAGGAAAGATGCTACTCAAGTTGTTGCAAACTTACAGAGGCAGCAGGTTCAATCCAAGCTGATTGCTTCTGATTACTTGGAAAGAAATATGGATCTTATGGATATTTTGATATCTAGCTATGAAAACCCAGAGATGGCTTTGCACTATGGTGCAATGTTAAGGGAGTGCATACGTCATCAGATTGTTGCAAAATATGTTCTAGAGTCACCTCATATGAAGAAGTTTTTTGACTACATTCAGATCCCAAACTTCGACATAGCCGCAGATGCCGCAGCAACTTTTAAGGAACTCATGACGAGACATAAATCTACTGTAGCTGAATTCCTTTCCAATAATTATGAATGGTTTTTTGATGAATATAACTCCAAACTATTGGAATCTTCCAATTATATTACAAGGCGTCAAGCTGTGAAGTTGTTGGGAGATATGTTACTTGATCGCTCAAATGCTGCTGTAATGACAAGATATGTCAGCTCCAGAGACAACTTAAGGATTTTAATGAATCTTATGAGAGAGTCAAGCAAGAGCATACAGATTGAAGCATTTCATGTTTTCAAGCTATTTGCTGCTAACCAAAAGAAACCACCAGATATCATCGGCATACTTGTTACCAACAGAAACAAGCTTCTGAGGCTGTTGGGCGACTTGAAAATCGATAAAGAGGATGAACAATTTGAAGCTGACAAAGCTCAAGTCATAAAGGAAATTGCTGATCTTGAACCTAGGGAATAGGGACAATCCTTGAACTCCATATTATGTCAAGAAGCAAGTTGTGCATCAGGCCATTTCATTGATTGTCTATTGTTACCGTTCTTTTAGTCTTCATTAAGTCGAGATTAATATTTCGCGCACATTTCTATGTTTCTGTTCCATAATTTTATTTGGTTCTGTAAATTTGGTAAATAAATAAATTGCAAAAATGAGATACATGTAATAGAAGTATATGGCTTTCTTGGTGTCTAGAAAGCTTGACTTTAGCAACTCAAGAGTTTATAAATTTTATTGAAAATTTGACCATCTACAAATTGTAGGGTTAAGTTTTAGTTGCTT
TARGET=85,25
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2573
PRIMER_LEFT_PENALTY=0.081670
PRIMER_RIGHT_PENALTY=0.175676
PRIMER_LEFT_SEQUENCE=AGCGTAGTTGAAAGAGGAGCAC
PRIMER_RIGHT_SEQUENCE=GATTTCGACAAGCACACACCTA
PRIMER_LEFT=37,22
PRIMER_RIGHT=205,22
PRIMER_LEFT_TM=60.082
PRIMER_RIGHT_TM=60.176
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=0.3564
PRIMER_LEFT_1_PENALTY=0.180765
PRIMER_RIGHT_1_PENALTY=0.175676
PRIMER_LEFT_1_SEQUENCE=AAGAGGAGCACAACAACACAAA
PRIMER_RIGHT_1_SEQUENCE=GATTTCGACAAGCACACACCTA
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=59.819
PRIMER_RIGHT_1_TM=60.176
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=0.3564
PRIMER_LEFT_2_PENALTY=0.180765
PRIMER_RIGHT_2_PENALTY=0.175676
PRIMER_LEFT_2_SEQUENCE=AAAGAGGAGCACAACAACACAA
PRIMER_RIGHT_2_SEQUENCE=GATTTCGACAAGCACACACCTA
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=205,22
PRIMER_LEFT_2_TM=59.819
PRIMER_RIGHT_2_TM=60.176
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.4050
PRIMER_LEFT_3_PENALTY=0.081670
PRIMER_RIGHT_3_PENALTY=0.323355
PRIMER_LEFT_3_SEQUENCE=AGCGTAGTTGAAAGAGGAGCAC
PRIMER_RIGHT_3_SEQUENCE=TCGACAAGCACACACCTATTTT
PRIMER_LEFT_3=37,22
PRIMER_RIGHT_3=201,22
PRIMER_LEFT_3_TM=60.082
PRIMER_RIGHT_3_TM=59.677
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.4050
PRIMER_LEFT_4_PENALTY=0.081670
PRIMER_RIGHT_4_PENALTY=0.323355
PRIMER_LEFT_4_SEQUENCE=AGCGTAGTTGAAAGAGGAGCAC
PRIMER_RIGHT_4_SEQUENCE=TTCGACAAGCACACACCTATTT
PRIMER_LEFT_4=37,22
PRIMER_RIGHT_4=202,22
PRIMER_LEFT_4_TM=60.082
PRIMER_RIGHT_4_TM=59.677
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=166
=
PRIMER_SEQUENCE_ID=SSR_05109 Ps_contig_mira-and-tgicl-ass_franssen_36472
SEQUENCE=AGTACGACTGTAAATTAAGTTATTAGTTATAGCGATTAGCGTAGTTGAAAGAGGAGCACAACAACACAAACAAAAAAACAGTTGGAAGAGAAGAGAAGAGAAGAGAAGATTAATCGATTCTCGATCGGAATAGAGAAAAACCCTAACGCAATCAATCAAATAGATGATTCAGAATTAGCAAAAATAGGTGTGTGCTTGTCGAAATCGAAGGGGCTTGCAAGAGCCTCTTCATCGGAGGTGGTGGTTTATTCCGAACCTCCACCTCCGACGGAATCCAAGAGCCTCTTCAAGCCTAAGCTCCGCACTCCTGCCGACATTGTCCGGAGCACTCGGGATCTTCTCCGTCTTCTCCAGAATGCTTCCGTTAACAAGCGCGATAACGACAAGATGATCGAGTTGTTTAAAAATTTTAAGAGAGATGAAAACAATTCTGTATGGAAATAGTGAAGCTGAACCAGTGCCGGAAGCTTGTTCGCAATTGACGCAGGAGTTCTTTAACGATAATACTTTTCGACTTCTGGTTGAGTGTCTTCCCAAATTGAACTTGGAGGCTAGGAAAGATGCTACTCAAGTTGTTGCAAACTTACAGAGGCAGCAGGTTCAATCCAAGCTGATTGCTTCTGATTACTTGGAAAGAAATATGGATCTTATGGATATTTTGATATCTAGCTATGAAAACCCAGAGATGGCTTTGCACTATGGTGCAATGTTAAGGGAGTGCATACGTCATCAGATTGTTGCAAAATATGTTCTAGAGTCACCTCATATGAAGAAGTTTTTTGACTACATTCAGATCCCAAACTTCGACATAGCCGCAGATGCCGCAGCAACTTTTAAGGAACTCATGACGAGACATAAATCTACTGTAGCTGAATTCCTTTCCAATAATTATGAATGGTTTTTTGATGAATATAACTCCAAACTATTGGAATCTTCCAATTATATTACAAGGCGTCAAGCTGTGAAGTTGTTGGGAGATATGTTACTTGATCGCTCAAATGCTGCTGTAATGACAAGATATGTCAGCTCCAGAGACAACTTAAGGATTTTAATGAATCTTATGAGAGAGTCAAGCAAGAGCATACAGATTGAAGCATTTCATGTTTTCAAGCTATTTGCTGCTAACCAAAAGAAACCACCAGATATCATCGGCATACTTGTTACCAACAGAAACAAGCTTCTGAGGCTGTTGGGCGACTTGAAAATCGATAAAGAGGATGAACAATTTGAAGCTGACAAAGCTCAAGTCATAAAGGAAATTGCTGATCTTGAACCTAGGGAATAGGGACAATCCTTGAACTCCATATTATGTCAAGAAGCAAGTTGTGCATCAGGCCATTTCATTGATTGTCTATTGTTACCGTTCTTTTAGTCTTCATTAAGTCGAGATTAATATTTCGCGCACATTTCTATGTTTCTGTTCCATAATTTTATTTGGTTCTGTAAATTTGGTAAATAAATAAATTGCAAAAATGAGATACATGTAATAGAAGTATATGGCTTTCTTGGTGTCTAGAAAGCTTGACTTTAGCAACTCAAGAGTTTATAAATTTTATTGAAAATTTGACCATCTACAAATTGTAGGGTTAAGTTTTAGTTGCTT
TARGET=1453,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1531
PRIMER_LEFT_PENALTY=0.170538
PRIMER_RIGHT_PENALTY=0.982604
PRIMER_LEFT_SEQUENCE=TGAACCTAGGGAATAGGGACAA
PRIMER_RIGHT_SEQUENCE=TTTCTAGACACCAAGAAAGCCA
PRIMER_LEFT=1269,22
PRIMER_RIGHT=1518,22
PRIMER_LEFT_TM=59.829
PRIMER_RIGHT_TM=59.017
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=1.1531
PRIMER_LEFT_1_PENALTY=0.170538
PRIMER_RIGHT_1_PENALTY=0.982604
PRIMER_LEFT_1_SEQUENCE=TTGAACCTAGGGAATAGGGACA
PRIMER_RIGHT_1_SEQUENCE=TTTCTAGACACCAAGAAAGCCA
PRIMER_LEFT_1=1268,22
PRIMER_RIGHT_1=1518,22
PRIMER_LEFT_1_TM=59.829
PRIMER_RIGHT_1_TM=59.017
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=251
PRIMER_PAIR_PENALTY_2=1.1696
PRIMER_LEFT_2_PENALTY=0.186949
PRIMER_RIGHT_2_PENALTY=0.982604
PRIMER_LEFT_2_SEQUENCE=GGGACAATCCTTGAACTCCATA
PRIMER_RIGHT_2_SEQUENCE=TTTCTAGACACCAAGAAAGCCA
PRIMER_LEFT_2=1284,22
PRIMER_RIGHT_2=1518,22
PRIMER_LEFT_2_TM=60.187
PRIMER_RIGHT_2_TM=59.017
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=235
PRIMER_PAIR_PENALTY_3=1.1759
PRIMER_LEFT_3_PENALTY=0.193337
PRIMER_RIGHT_3_PENALTY=0.982604
PRIMER_LEFT_3_SEQUENCE=TTTGAAGCTGACAAAGCTCAAG
PRIMER_RIGHT_3_SEQUENCE=TTTCTAGACACCAAGAAAGCCA
PRIMER_LEFT_3=1225,22
PRIMER_RIGHT_3=1518,22
PRIMER_LEFT_3_TM=59.807
PRIMER_RIGHT_3_TM=59.017
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=1.1797
PRIMER_LEFT_4_PENALTY=0.197140
PRIMER_RIGHT_4_PENALTY=0.982604
PRIMER_LEFT_4_SEQUENCE=TGAAGCTGACAAAGCTCAAGTC
PRIMER_RIGHT_4_SEQUENCE=TTTCTAGACACCAAGAAAGCCA
PRIMER_LEFT_4=1227,22
PRIMER_RIGHT_4=1518,22
PRIMER_LEFT_4_TM=59.803
PRIMER_RIGHT_4_TM=59.017
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_05110 Ps_contig_mira-and-tgicl-ass_franssen_36476
SEQUENCE=ATTGACTTGTAAACAATTTCATAAACTTTAAGAGACTTCTACATTATAAGAGATTAGAAAAGAACAAGCTCCTTTTATTTGAAAACAAATGTGAAATGTGAGAGTAAGAAATAAATAGAACCCATCTTACATTAAAAGAGTCAAAAAATTATGAGACTTGGAAGATAAAAAACAAGTAGCAAGCTTATTCTATATGCTTAGTAAAAACTAAAATAAACCTAAAACAAATAGTTTTCATCACCTAACATTGCTCTCAAAACAGTTTCTTTCTGTAAACCAATTCTCCTTAGAAAGAACCGTGCATCTCTTCTTAATTGTTGCGAGGAGTCGAGGCTTCGGATGGACTATTTGACGAGTCACTGGTGAGGGAACCGTTGCCACCATCGTTCTCAGAGACCACTGGTATCTTCACAGCGGCTGCTCTTTCCATCTCTTTCTCGAGTTCTTCCTCTTTACGCAACGCGGTAAACTGTGTATCCAATGACTTGGCTGCTCCTAACCAGGCAAGAACAATCACTAGAAGCACACCTCCCAGGTATGGAGTTGAATTGGCGAGTGATCCGAAAGTTAGGATCATGAATTGTTGAATAAGGGCACCTCCGGATTTTCCCAATGGGTTGCAAACAACATCAATCGCTGCTTTGCCTTTGACCTTGGTATCCTCATCCAAGGGAATGTAAGCCATTTCTTTGCAGGGATCGAACAAGCTGTACTTAGCACTCTTGCTAAAGATGTTTTGCAAGGCACCGACATAAACAGCTGCTAGCAGTGGAGTCATTCCAAGTGATGCAAGACCAGGTGCAATTGGACCTCCAAACAATATGAGAGAGAAGAAACCGACTCCGGTTAGAAGCAAGACGGTAGGTGTGATCTTGGCAGCAACTCCCCAACCATATTTGTCAAATATATATTGACTTAGAAGCATCATTATGAACGTGGCAATTCCAGTTGCAGTCGAGAAGTCACCCATGAAAGAAGAGTACTCGTTGGGGCTAGGAAACTGAGCTTTGAGCTTAGACTTCCATGTAACCTCGACAAGATTGATACTGATTCCGTAAGCAACCACTAAAGTGGCAAGATCTCTGATGTATCTTGAAGACACCAAGAATTTCAAGCTCTCCATTGTTCCCATTTTGGGCTTCTTCTTCTTCTTCACGCTACGTTCTGGAAGAGGGACGAAATTATTCGTCCACCAGTACAGGCCACAGATTGCAAATCCCATGAGCACAACTATGCTCATCATTCCCTTTAGAGAGATGGCCCAACCATCAACACCGGGACCTAAATTCTTCCTCATATTAGAAAAGTACTTCACGGTACGACCAGAGAAAATAAGAGCTACATTGGCACCAAGTCCAAACAGTGGGTAGAATCGTTTTGCTTCGTCAATGGTAGTTATCTGATTAGCAAAACCCCAAAAGAGCACAGAAACAACCACACTCCCCCACAATTCAGCCATGACATAGAACAAACAGAAACTCCAAATTCTGATAATTGCAAGAGGACCAAGAAACCTAGGACCAAGAACACTAAGAAGCTTGTCTGCAAAAGCCTCAGGGTGAATATAGTTGCTAAGAGGATACAAAACAAAACCAAAAGCCCCAAAGAAAGCAATAAAAGGGAGAATCACAGTGTAAAAAAGTGCCTGTTTTGACAAAACATTAGACAACTTTGTATACAACAACATAAACCCAATAGCCATAGGAAGATTCACCCATGTTTTCAAAAACGGTATAATCTCAGCACTACTCCCCTTTGCAGTAACAACCAAAACATCCTTAGTATCCCTCAAAATCGTATAATTAAAAAGGATGCAAAAGAACATCAACCCTAAAGGTAAAATCTTTTTCATTGTATCAACCTCAATCCCCAAAATTTTAGGTTTTTTCTCAACCTCAGCCTCCCCAAAAACTGGTTGTTGTCCATCAGAAGCAGCAGCCGCCTCTGCTTTACAGATGAACAAATTCCTCTCGTTTTGAACCNAAAACCTATTTAACTTTTGGAGAAAACRTNGTAGTTTACAAACCTNTTGAACTGAAGAAGAAACGAAACCCAAACGGGTTAAAGGGGGGTTTTTTAGGGGGTTTTAAAAG
TARGET=1139,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05111 Ps_contig_mira-and-tgicl-ass_franssen_36483
SEQUENCE=CGGTAAAAGTAAAAAAAAAAAGGAAAAAACTAACTCCTCCACACATCACCCTCCGCCGTTCACCGCCTGTCACAAACCACCTTCACGCATCCGTCGGCATCACACAACCAACGTCCTCCACACATTAAATCTCCCTAGATCTCATCTCCGGCGAACACCAACAAGCGCCGTGAAATCGACGCGCCACCGCCTCTCCACAACTCCTCAACCGTCTCGCCAACGATTCTCACAATCAAACACTCACCGTCACAACCACCAAAATCCTTCCATTTTGCGTTTCCTCCGTCTTCATCTTAACCGTCGGCATAAACTTACTCTCGTTTCCACTCGCCCTCAACTATGAAATAACCACACGCATCCCTTCCATCGTCTACATGACTACTCAAAAGCGGATTTGTGACGGTGCCGTTAGATATGGATAAAGATGTCTACAGCTTCTAAACCCACAACCGGAACATTCCGAATACGTTGAAGGAAAGGGAATACAATCCGTTGAAATCTCGAAAAAAGAAGAAGCTACAACCGAACTTCGGCAACAATGTTACGATGTCGGTAAATTTTGAACTTACTTCTCTTCGACTCTGTTTACATCACGTATGTTGCTCCAATATTGGTTTTGATTTGGATTCTGTTGTATTGCTGGATGGTTGTGTGTGTGGTTATTTCAATTACACAGTATTTTCTTATTCAATTTTGTGTGGTGTTTAATTATTTATGTTGAAGTTAATTAAGTATATTATGTTGGATATCTTAAGGTCACGGTTGGCATTCTGGTTTTAATAATAGCATTTGGTGACTTATGTTCATGTTAAATTAAGTGGATGACTTCTAAATTTGGTATGTTGTTTTTAATAA
TARGET=649,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2466
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=1.242983
PRIMER_LEFT_SEQUENCE=TTGGATTCTGTTGTATTGCTGG
PRIMER_RIGHT_SEQUENCE=CAACCGTGACCTTAAGATATCCA
PRIMER_LEFT=621,22
PRIMER_RIGHT=764,23
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.243
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=1.3716
PRIMER_LEFT_1_PENALTY=0.128591
PRIMER_RIGHT_1_PENALTY=1.242983
PRIMER_LEFT_1_SEQUENCE=ACGTTGAAGGAAAGGGAATACA
PRIMER_RIGHT_1_SEQUENCE=CCGTGACCTTAAGATATCCAACA
PRIMER_LEFT_1=465,22
PRIMER_RIGHT_1=761,23
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=60.243
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=1.3716
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=1.242983
PRIMER_LEFT_2_SEQUENCE=ACGTTGAAGGAAAGGGAATACA
PRIMER_RIGHT_2_SEQUENCE=CAACCGTGACCTTAAGATATCCA
PRIMER_LEFT_2=465,22
PRIMER_RIGHT_2=764,23
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=60.243
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=1.4268
PRIMER_LEFT_3_PENALTY=0.183834
PRIMER_RIGHT_3_PENALTY=1.242983
PRIMER_LEFT_3_SEQUENCE=GAAAGGGAATACAATCCGTTGA
PRIMER_RIGHT_3_SEQUENCE=CCGTGACCTTAAGATATCCAACA
PRIMER_LEFT_3=474,22
PRIMER_RIGHT_3=761,23
PRIMER_LEFT_3_TM=60.184
PRIMER_RIGHT_3_TM=60.243
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=1.4268
PRIMER_LEFT_4_PENALTY=0.183834
PRIMER_RIGHT_4_PENALTY=1.242983
PRIMER_LEFT_4_SEQUENCE=GAAAGGGAATACAATCCGTTGA
PRIMER_RIGHT_4_SEQUENCE=CAACCGTGACCTTAAGATATCCA
PRIMER_LEFT_4=474,22
PRIMER_RIGHT_4=764,23
PRIMER_LEFT_4_TM=60.184
PRIMER_RIGHT_4_TM=60.243
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_05112 Ps_contig_mira-and-tgicl-ass_franssen_36484
SEQUENCE=GTCGGGGGATTTTTCTCTCTGTTGAGAATCACCTCAAAAGTCCCTCTTCTATCTCCACACGTCTCACACACGTTTCTCTCAACAAAATTACTACCTCTCTTCTCTCACTATTCAACTACACACTCTGAAAGAAAAGAGAAGAATTAATAAAAAAAACTTCTTCTTCTTACCCACAACACCTTCACTCCGACCGCCGCTTTTCCTTCACCAACACACTCCGCCGACAACTTCCGGCGAAGCTATACTTCATCTTCTTCAACGCACACCACCACCGGAGGCCACACCCTCCGACGACACCCTTTCTCATCGTTGCCGACACACACCGGCAAATCGCCGACCGCGCCTACACCCACCGTCGTCCGTCAACAGCCCCTCACAATCCACGATTCATACCGACAACAACCCTCCGTCATGACTCTAAACACCACTCAACCTCAACCGCCGCTTTCCATAAACCTCCTTGGCCACCGTGAAACACCTCCGATAAGAGCGACAACCACTGACTGGTTTATTTCCAATCTTCCTCTTTTCTTTTCACCTCTGTTATTCATCACTAACAACATTATGCCACCGTCGAAACCTTTCGAGTTCAACCATCAGAGAGTTGAAAGCGTAAATAGCATCGTGTAGGTAATGTTGTTCGATTCTCATATGTTCAAATGTTTATTGTTTACAAAGTCGTATATATGCCCATTCACATTTTTTTTCATGAATAAATGTCAATTTATTTAGCTTATGCAGTATGAATCATGCACAATTATTTCAAACTGTGATTGCTTTATTTTTCTTTCTATGAAGTTCCTAAAAAATTTCCGGCGAGTTATAGAAATTGGTTGAACACTTTTTAGGAAATGACTTGTAGTTACTTTGCTTTTGTTATTTTCCTTGGATCTGAGAAAGAGGGAAGGAAAGATTATGTGGTTTGAAGTTAATGATTTAAATAGGAGAGAGATAAGGCATTTTCGTTTCATTTTATGAGCATTTACCATTGTTTTTATTATTTATTTAATGAGCATTGCATTGTCACATGTCTGTTGGTTATTGGATAATTACTAAAAGTTNGAAAATGTTATAA
TARGET=157,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3451
PRIMER_LEFT_PENALTY=0.265389
PRIMER_RIGHT_PENALTY=0.079714
PRIMER_LEFT_SEQUENCE=ACACGTCTCACACACGTTTCTC
PRIMER_RIGHT_SEQUENCE=GTGTGCGTTGAAGAAGATGAAG
PRIMER_LEFT=56,22
PRIMER_RIGHT=265,22
PRIMER_LEFT_TM=60.265
PRIMER_RIGHT_TM=59.920
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.3451
PRIMER_LEFT_1_PENALTY=0.265389
PRIMER_RIGHT_1_PENALTY=0.079714
PRIMER_LEFT_1_SEQUENCE=ACACGTCTCACACACGTTTCTC
PRIMER_RIGHT_1_SEQUENCE=TGTGCGTTGAAGAAGATGAAGT
PRIMER_LEFT_1=56,22
PRIMER_RIGHT_1=264,22
PRIMER_LEFT_1_TM=60.265
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.4811
PRIMER_LEFT_2_PENALTY=0.401426
PRIMER_RIGHT_2_PENALTY=0.079714
PRIMER_LEFT_2_SEQUENCE=CACGTCTCACACACGTTTCTCT
PRIMER_RIGHT_2_SEQUENCE=GTGTGCGTTGAAGAAGATGAAG
PRIMER_LEFT_2=57,22
PRIMER_RIGHT_2=265,22
PRIMER_LEFT_2_TM=60.401
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.4811
PRIMER_LEFT_3_PENALTY=0.401426
PRIMER_RIGHT_3_PENALTY=0.079714
PRIMER_LEFT_3_SEQUENCE=CACGTCTCACACACGTTTCTCT
PRIMER_RIGHT_3_SEQUENCE=TGTGCGTTGAAGAAGATGAAGT
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=264,22
PRIMER_LEFT_3_TM=60.401
PRIMER_RIGHT_3_TM=59.920
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.5554
PRIMER_LEFT_4_PENALTY=0.475706
PRIMER_RIGHT_4_PENALTY=0.079714
PRIMER_LEFT_4_SEQUENCE=TGAGAATCACCTCAAAAGTCCC
PRIMER_RIGHT_4_SEQUENCE=TGTGCGTTGAAGAAGATGAAGT
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=264,22
PRIMER_LEFT_4_TM=60.476
PRIMER_RIGHT_4_TM=59.920
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_05113 Ps_contig_mira-and-tgicl-ass_franssen_36487
SEQUENCE=CCAACACGAGAACTAAAAACCAAGCACAATAAGCAGCACTAAACGCCGCAAAAACGACGCTGTTTAAGAACGGGTAAGTACAGAGAACGGTGTTGTTTTCAAGAACCACCGATGTTTGTCTCAAGAACGCCGGAACCTGATTCCCCAACGGATTATAGAAAACAAGCAAACCGTGAAGAACAACCTGAGGAACACACGTGAAGAACACGAATGTAATAGCCCAAGCGTCGTTCGGTGTTTTTTTCCTAATCGAAGCGTTAAGGAGAAAAAGTAGCGTCACAAGAAAAGCCGGTTCGAAGAATCCAAGAGTAAGAACAACGCTGAGTTTACAGAGATTCGCTTGTTGATAAACCTCCAACGAGGGTATATATGGAGATAAAAATCTTCTACGGAAGAACGGTAACCGGAGAAGTTCGACAACCGACCAGAGAAAAATGAAAAGAACGAGAAGAAACCGAACGGTCCAGAGGGAATTGAAGTTTTGTAAATAGGGTACCGATTTGGATTTGAAGCGAAGGTGAAAAATGAAACAGAGAGATAAAACAGAGAGTAATGATAAGAGAGGAGATAAACGTTACGGTGGTGATGTCGAAAGCTGAATTAAGGACTGGTGATGCAGTCATGAACAACATGGATCGAATATTGGAACAATTTCGATCGCTGTTATTTTTGTTTTTGTTTATGTGCCAGCATAAATATGAATATGAATATGAATTAGTTTGAGTAGATGATTGTTAGAGAAATTAGAAAATGTCGATCCTGGTGGCGGTGGTCGGAGAGGTGGTGGAGAGAGAGTGAGGTTAAGCATGGAAGAAGAAGAAAATGAATATGAATATCGTTGTTAGGAGAATGAAGAGGCATGTGGGTTTGGTTTGGGTTTAATTTTGGGCCGGATGGGACAGCGGGGATAAGAGATATAAAAGATTTGAATGAGTAATTTCCGTGTRTGTTTTGTCTCTGTCTCTCCACTCNCCCAACGTGGTGGANAGACNGC
TARGET=810,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05114 Ps_contig_mira-and-tgicl-ass_franssen_36489
SEQUENCE=TCGGGGTACCTAAAAAATTCTTACTTTAAGTTCTATTCATTTCCTTTTCCGTTGGGGAAGCACCAACAAGCAAATCCTATAACAACCAAACCAAGCTACCATTACAAACCCTCTTTGAATTGCACTCCATCTCAAACATGTAACGTAGCTTTCACTCCTCTACCCCATATGGAACCATTATTTCTTGAGAAAACTTGGGGTAGTGGAGAAAGTGGATGGTGTGTGTGTGTGTTAATGGTTTCCAATTTCCATGCTTCCTTTTCTTTCATGACTCTATGTTAAAGATTGATGGGAACGAACCTTCTTTGTAGATGGGAAATTCTCTTTGGAAAGGGCAACTTCGATCCTTTGGCATTTTTCAATTCCTTCATCTTGTGAGATTTGATGAAGCTTTCATAATGGAAACACTTCTATTGTGTAATATGTATTGTACTCTCTTCTTTTCCTATAATCTTTTTTGTGTGTGTGTTGTTATGTGATGATAACTTCTAAGTTGTAACTGCAAGCTATGTTTGTAAGACATGCCTACTATAGACTATAGTATTAGTAGGTATATCCAATAAATGTACGTTTACTTATGCCACTTGGCAAATATTTATTATTGCTGTTGTTTCTAACTCTATGAGCCTAATCATGTTTAATTTTTAAAACT
TARGET=219,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1563
PRIMER_LEFT_PENALTY=0.027715
PRIMER_RIGHT_PENALTY=0.128591
PRIMER_LEFT_SEQUENCE=AGCACCAACAAGCAAATCCTAT
PRIMER_RIGHT_SEQUENCE=TACAAAGAAGGTTCGTTCCCAT
PRIMER_LEFT=58,22
PRIMER_RIGHT=309,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=59.871
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.2115
PRIMER_LEFT_1_PENALTY=0.027715
PRIMER_RIGHT_1_PENALTY=0.183834
PRIMER_LEFT_1_SEQUENCE=AGCACCAACAAGCAAATCCTAT
PRIMER_RIGHT_1_SEQUENCE=GGTTCGTTCCCATCAATCTTTA
PRIMER_LEFT_1=58,22
PRIMER_RIGHT_1=300,22
PRIMER_LEFT_1_TM=60.028
PRIMER_RIGHT_1_TM=60.184
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.2464
PRIMER_LEFT_2_PENALTY=0.117817
PRIMER_RIGHT_2_PENALTY=0.128591
PRIMER_LEFT_2_SEQUENCE=GAATTGCACTCCATCTCAAACA
PRIMER_RIGHT_2_SEQUENCE=TACAAAGAAGGTTCGTTCCCAT
PRIMER_LEFT_2=116,22
PRIMER_RIGHT_2=309,22
PRIMER_LEFT_2_TM=60.118
PRIMER_RIGHT_2_TM=59.871
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.2464
PRIMER_LEFT_3_PENALTY=0.117817
PRIMER_RIGHT_3_PENALTY=0.128591
PRIMER_LEFT_3_SEQUENCE=TGAATTGCACTCCATCTCAAAC
PRIMER_RIGHT_3_SEQUENCE=TACAAAGAAGGTTCGTTCCCAT
PRIMER_LEFT_3=115,22
PRIMER_RIGHT_3=309,22
PRIMER_LEFT_3_TM=60.118
PRIMER_RIGHT_3_TM=59.871
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=0.3017
PRIMER_LEFT_4_PENALTY=0.117817
PRIMER_RIGHT_4_PENALTY=0.183834
PRIMER_LEFT_4_SEQUENCE=GAATTGCACTCCATCTCAAACA
PRIMER_RIGHT_4_SEQUENCE=GGTTCGTTCCCATCAATCTTTA
PRIMER_LEFT_4=116,22
PRIMER_RIGHT_4=300,22
PRIMER_LEFT_4_TM=60.118
PRIMER_RIGHT_4_TM=60.184
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_05115 Ps_contig_mira-and-tgicl-ass_franssen_36489
SEQUENCE=TCGGGGTACCTAAAAAATTCTTACTTTAAGTTCTATTCATTTCCTTTTCCGTTGGGGAAGCACCAACAAGCAAATCCTATAACAACCAAACCAAGCTACCATTACAAACCCTCTTTGAATTGCACTCCATCTCAAACATGTAACGTAGCTTTCACTCCTCTACCCCATATGGAACCATTATTTCTTGAGAAAACTTGGGGTAGTGGAGAAAGTGGATGGTGTGTGTGTGTGTTAATGGTTTCCAATTTCCATGCTTCCTTTTCTTTCATGACTCTATGTTAAAGATTGATGGGAACGAACCTTCTTTGTAGATGGGAAATTCTCTTTGGAAAGGGCAACTTCGATCCTTTGGCATTTTTCAATTCCTTCATCTTGTGAGATTTGATGAAGCTTTCATAATGGAAACACTTCTATTGTGTAATATGTATTGTACTCTCTTCTTTTCCTATAATCTTTTTTGTGTGTGTGTTGTTATGTGATGATAACTTCTAAGTTGTAACTGCAAGCTATGTTTGTAAGACATGCCTACTATAGACTATAGTATTAGTAGGTATATCCAATAAATGTACGTTTACTTATGCCACTTGGCAAATATTTATTATTGCTGTTGTTTCTAACTCTATGAGCCTAATCATGTTTAATTTTTAAAACT
TARGET=459,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2865
PRIMER_LEFT_PENALTY=0.474473
PRIMER_RIGHT_PENALTY=0.811989
PRIMER_LEFT_SEQUENCE=CGAACCTTCTTTGTAGATGGGA
PRIMER_RIGHT_SEQUENCE=TTGCCAAGTGGCATAAGTAAAC
PRIMER_LEFT=295,22
PRIMER_RIGHT=590,22
PRIMER_LEFT_TM=60.474
PRIMER_RIGHT_TM=59.188
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=1.2907
PRIMER_LEFT_1_PENALTY=0.478666
PRIMER_RIGHT_1_PENALTY=0.811989
PRIMER_LEFT_1_SEQUENCE=ACGAACCTTCTTTGTAGATGGG
PRIMER_RIGHT_1_SEQUENCE=TTGCCAAGTGGCATAAGTAAAC
PRIMER_LEFT_1=294,22
PRIMER_RIGHT_1=590,22
PRIMER_LEFT_1_TM=59.521
PRIMER_RIGHT_1_TM=59.188
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=1.7080
PRIMER_LEFT_2_PENALTY=0.368624
PRIMER_RIGHT_2_PENALTY=1.339425
PRIMER_LEFT_2_SEQUENCE=GGGAACGAACCTTCTTTGTAGA
PRIMER_RIGHT_2_SEQUENCE=GCCAAGTGGCATAAGTAAACG
PRIMER_LEFT_2=290,22
PRIMER_RIGHT_2=588,21
PRIMER_LEFT_2_TM=59.631
PRIMER_RIGHT_2_TM=59.661
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=1.8139
PRIMER_LEFT_3_PENALTY=0.474473
PRIMER_RIGHT_3_PENALTY=1.339425
PRIMER_LEFT_3_SEQUENCE=CGAACCTTCTTTGTAGATGGGA
PRIMER_RIGHT_3_SEQUENCE=GCCAAGTGGCATAAGTAAACG
PRIMER_LEFT_3=295,22
PRIMER_RIGHT_3=588,21
PRIMER_LEFT_3_TM=60.474
PRIMER_RIGHT_3_TM=59.661
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=1.8181
PRIMER_LEFT_4_PENALTY=0.478666
PRIMER_RIGHT_4_PENALTY=1.339425
PRIMER_LEFT_4_SEQUENCE=ACGAACCTTCTTTGTAGATGGG
PRIMER_RIGHT_4_SEQUENCE=GCCAAGTGGCATAAGTAAACG
PRIMER_LEFT_4=294,22
PRIMER_RIGHT_4=588,21
PRIMER_LEFT_4_TM=59.521
PRIMER_RIGHT_4_TM=59.661
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_05116 Ps_contig_mira-and-tgicl-ass_franssen_36499
SEQUENCE=CCYCTTATAATCCTTATATTTAAACATTACCATAAACAATACTCAATGTATATGTATACAATATTATACACCATAGCCTTGTATCTATAGCTAGCCCATTTTAGCATGGCCATACATTGAAATTGAGTCAAACCTGTTGCATGATTACACAATTACAGTGCAGGTATTGATCATATGTATATTTATAGGCTTCCTACAACAACATGTATCTGTGTTTTTTAATAAAGAAAACTGATACAATGTAACTGTCCTCTCATCTATTCATTTATAGGACGAATCATCCACGGCGGAAGAACGGCACTTCCTCGAGTTTCTGCTCCGGTTTCTTCGTTGCAATTTTCTTGTAAGGAGGACCCTCCTGCAATCATGGATTCCAATGGTTGAGTTTCAATTAGAGCCACCTCCATGCTATTTTGCACCTCCTCTTGAGCAAGTTCTTTCTCTTTTTCCTTTATCTTCTTGGCGAGCAAGTTGTTATGTTCTTGGAGTGCCTTATCCTTTTTCTGAAGTTCTGAAATAGATTCATACATGACTTGATTCTTCCGTGACCTAATGTGTTTGAGAGCAGAATCAAGTTGTTGTTCCAAACTTTGAAGCTCTTTGAGACTTAACCCATCCAACTCTTCTCCCATATAATTCCTTTGATTTTTCTGAAGTACTTCCACCCTAGCTTTCAGTTTTGCATGTTCTAGAACCCAGTTTTCATTTGGTGATTGATCATTTGCAACAAGCTGTCTCTCCGTATAAGAATATCTCTCATATCGTTCAAGAATTCTTTCCATACAAGGATCGCTAGAGTATTCAAAAAGCTTGCCTTTAGTAGAGAAAACTATGAGAGCAACTTCAGCATCACAAAGAACAGAGATCTCATGAGCTTTCTTCAACAAACCAGACCGTCTTTTCGAAAAAGTTACTTGTCTATTGATTTTGTTCTCAATCCTCTTCAACTGCACTCTTCCTCTCCCCATCTTTATTATCTCTCTCTCAAACCACAATTCAAATCACACCAACATGAATTGAAGCTATATCTATAGCTAGATAACTACATACACTATAGAGATATATGATAAATGTTTTATTATTTGGATTTTTTTGTTGTTGATGTAGATAATTTGTGATATGAATGAATGATAATATGAAAATGAAGGGGTTTGAGCACAAAAAACGTAGCTCCTTCGTT
TARGET=977,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05117 Ps_contig_mira-and-tgicl-ass_franssen_36499
SEQUENCE=CCYCTTATAATCCTTATATTTAAACATTACCATAAACAATACTCAATGTATATGTATACAATATTATACACCATAGCCTTGTATCTATAGCTAGCCCATTTTAGCATGGCCATACATTGAAATTGAGTCAAACCTGTTGCATGATTACACAATTACAGTGCAGGTATTGATCATATGTATATTTATAGGCTTCCTACAACAACATGTATCTGTGTTTTTTAATAAAGAAAACTGATACAATGTAACTGTCCTCTCATCTATTCATTTATAGGACGAATCATCCACGGCGGAAGAACGGCACTTCCTCGAGTTTCTGCTCCGGTTTCTTCGTTGCAATTTTCTTGTAAGGAGGACCCTCCTGCAATCATGGATTCCAATGGTTGAGTTTCAATTAGAGCCACCTCCATGCTATTTTGCACCTCCTCTTGAGCAAGTTCTTTCTCTTTTTCCTTTATCTTCTTGGCGAGCAAGTTGTTATGTTCTTGGAGTGCCTTATCCTTTTTCTGAAGTTCTGAAATAGATTCATACATGACTTGATTCTTCCGTGACCTAATGTGTTTGAGAGCAGAATCAAGTTGTTGTTCCAAACTTTGAAGCTCTTTGAGACTTAACCCATCCAACTCTTCTCCCATATAATTCCTTTGATTTTTCTGAAGTACTTCCACCCTAGCTTTCAGTTTTGCATGTTCTAGAACCCAGTTTTCATTTGGTGATTGATCATTTGCAACAAGCTGTCTCTCCGTATAAGAATATCTCTCATATCGTTCAAGAATTCTTTCCATACAAGGATCGCTAGAGTATTCAAAAAGCTTGCCTTTAGTAGAGAAAACTATGAGAGCAACTTCAGCATCACAAAGAACAGAGATCTCATGAGCTTTCTTCAACAAACCAGACCGTCTTTTCGAAAAAGTTACTTGTCTATTGATTTTGTTCTCAATCCTCTTCAACTGCACTCTTCCTCTCCCCATCTTTATTATCTCTCTCTCAAACCACAATTCAAATCACACCAACATGAATTGAAGCTATATCTATAGCTAGATAACTACATACACTATAGAGATATATGATAAATGTTTTATTATTTGGATTTTTTTGTTGTTGATGTAGATAATTTGTGATATGAATGAATGATAATATGAAAATGAAGGGGTTTGAGCACAAAAAACGTAGCTCCTTCGTT
TARGET=1120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05118 Ps_contig_mira-and-tgicl-ass_franssen_36501
SEQUENCE=AACCCGAACGTGTATCAGACCCTAATCCCTTTACCCCTTACCNGNTNTTCCCCTTTCCCGTACTTTCGTATCTCGATTTTGTATCTCGATTTGTTTCTTCCTCCCGATCTCGATTTCCATCCCCACCACCACCACCACCACCACTTCTCTCGTGAAAATTTCGTCATGGATCTCTTTCAACAGCGTGTAGAGCAATCTCACGTCATCGATCACACCGATCCTATCCATCAGTCCTTAAACGACTCCGTTTTCGACAGTTTGAGTCTCGATTTAGGGTTAAACTCTACCGATCTATACATCGAGGACGATGATTTTACCGATTCGAGGGTTTCGAGATCAGATCCAATCAACGGCTTGCGTATCGTTGAGATTGATTCGGAATCTGACGGTGAGGAAGTAGACGGAGACGGAAACGGGGACAACGTGTTGTATGGGATTTGCGTGCATTCTGATGAAGAAGAGTTTAGCAATGTAATCGATGATGTTACGAATTTTCCTTTCCGTTGGGATTCTCTTCAGTTGGATGATAATCAGATATATGAAGATTTTGATTGGGAGGAAGTTGACGGTCGTTTAGACGGGATGCCGGTTGGTAACGGCAACAATGTCAACAATAGGGCAGTTAGCGCTGTTCGTGATTTTCCGTATATCGAAGAAGAATCAGAAGAAGACGGAGGAGAGCTTACATTGACAAGGGTTGGTGAAGGCATGGGGAATCTCGAGTGGCAAGTGTTGTCGAATTCGGACAATTTAGGCACAAGCCCTGAAACTTACCATGTAATTGCAGAGCCTTTTGAGGACAATGATGATTACATTTACACTGCAGAGTATGAGATGATGGTTGGTCAATTCATAGGTAATGATAACCCTTCTACGGGGAGGCCTCCCGCTTCTGTTACTGTTGTGCAGAATCTTCCTTTGGTGGTTGTGACCAAAGAGGACGTGGACAACAACAATGCTCTGTGTGCAGTCTGTAAAGATGAATTTGCTGTTGGAGATGAGGTGAAGTTGTTGCCGTGTACTCATCGATACCATGGCGATTGCATTGTGCCCTGGTTGGGGATTAGGAATACTTGTCCTGTTTGTCGCTATGAGTTCCCCACAGATGATGCTGATTATGAACGTAGGAAAGCGCCAATGCTTGCCCGGAGCGCTTGATTGTATGTCATTGTTGCTAGGCTGTTGAGAGTTTAGGTTTTTTGGTCCTGAAATATTGCATGCCTTTGTTAATTTTTGAATTTTTCATATGGAGATTTTTTATTATAGGTATGTTATCTTCCAATTGTATTTTAGGATACATCATAAAAATGAATCAACTTTCTAAGGAATACATGAATTTGTTATGCAATCTGATTGTTCATATTCTTATGGGTTGTTATCCTTAAG
TARGET=123,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3133
PRIMER_LEFT_PENALTY=0.309343
PRIMER_RIGHT_PENALTY=0.003959
PRIMER_LEFT_SEQUENCE=ATTTGTTTCTTCCTCCCGATCT
PRIMER_RIGHT_SEQUENCE=GTGTGATCGATGACGTGAGATT
PRIMER_LEFT=88,22
PRIMER_RIGHT=214,22
PRIMER_LEFT_TM=60.309
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=127
PRIMER_PAIR_PENALTY_1=0.3396
PRIMER_LEFT_1_PENALTY=0.309343
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=ATTTGTTTCTTCCTCCCGATCT
PRIMER_RIGHT_1_SEQUENCE=TCGAGACTCAAACTGTCGAAAA
PRIMER_LEFT_1=88,22
PRIMER_RIGHT_1=268,22
PRIMER_LEFT_1_TM=60.309
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=0.3454
PRIMER_LEFT_2_PENALTY=0.341440
PRIMER_RIGHT_2_PENALTY=0.003959
PRIMER_LEFT_2_SEQUENCE=TAATCCCTTTACCCCTTACCNG
PRIMER_RIGHT_2_SEQUENCE=GTGTGATCGATGACGTGAGATT
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=214,22
PRIMER_LEFT_2_TM=60.341
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.75
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=193
PRIMER_PAIR_PENALTY_3=0.3617
PRIMER_LEFT_3_PENALTY=0.309343
PRIMER_RIGHT_3_PENALTY=0.052366
PRIMER_LEFT_3_SEQUENCE=ATTTGTTTCTTCCTCCCGATCT
PRIMER_RIGHT_3_SEQUENCE=ATTGCTCTACACGCTGTTGAAA
PRIMER_LEFT_3=88,22
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=60.309
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=108
PRIMER_PAIR_PENALTY_4=0.3717
PRIMER_LEFT_4_PENALTY=0.341440
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=TAATCCCTTTACCCCTTACCNG
PRIMER_RIGHT_4_SEQUENCE=TCGAGACTCAAACTGTCGAAAA
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=268,22
PRIMER_LEFT_4_TM=60.341
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.75
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.5000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_05119 Ps_contig_mira-and-tgicl-ass_franssen_36504
SEQUENCE=AACCNCNACACTATTGAGTCGAGATTTTACACAACCAGAAAGAAAATAGCCACAAAAACTCTTTCTTCTTTCCCTTTCCAAAATTTTTCCGATTCCAATGTCTTCTTCTTCAGAAGGTTACGCGATCGAGCTCTACATGGATCCCGCGCTCGAAAACCAAGTTCTGAAAGCCTGGAACGTTCTCGCCCGACGCCAAATCAGCACCGAGTTAATCGAAATCGGGTCACGGCCACACATCACACTCTTCTCAACTCCTTTCCTTCTAGAACCTTCCAAACTCGAGTCACTTCTCAAATCCCTAACTTCCAAATTCGATCCGTTTTCTCTCTCTTTCTCTTCGATCTCTTCTTTCTCCAACGATAACAACCTTCTCTTTCTCTCACCTACTCCTTCTATCTCTCTCCTCAATTTTCATTCTCATCTTGTGGATGCGGTGGTCAAGGAAGGGATTGAAGTTTCTCAGGAGTTTTTGGTAGGGAATTGGATTCCTCATTGCGCCGTTGCGAAGCGTGTTCCCAAGCAGAGAATGTCTGAAGCCTTTTCGCTTCTTAGAGACCTTAAATTGCCGGTTCAAGGATATGCTGTTGATGTTGCGCTCGTGCAATTCTCGCCGGTTCGTGAGATTTTCTCCTTTGTGCTTGGTAACAACAGTAATATTATAGAGAATTGATTTTATTTTATTTTATTTGAATCTTTTAGTAGCTTAATTTTGTCTGTATGTTAGTTTGTTGTAATAAGATATTTTTCTTGTTGTTGTTGTTGTTGATGATGATAATTAAGTAATTCTTGAATTGAAACTAGTTTTGGAGGAATTGTTCATGAATTATGTTGATTAGT
TARGET=670,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05120 Ps_contig_mira-and-tgicl-ass_franssen_36504
SEQUENCE=AACCNCNACACTATTGAGTCGAGATTTTACACAACCAGAAAGAAAATAGCCACAAAAACTCTTTCTTCTTTCCCTTTCCAAAATTTTTCCGATTCCAATGTCTTCTTCTTCAGAAGGTTACGCGATCGAGCTCTACATGGATCCCGCGCTCGAAAACCAAGTTCTGAAAGCCTGGAACGTTCTCGCCCGACGCCAAATCAGCACCGAGTTAATCGAAATCGGGTCACGGCCACACATCACACTCTTCTCAACTCCTTTCCTTCTAGAACCTTCCAAACTCGAGTCACTTCTCAAATCCCTAACTTCCAAATTCGATCCGTTTTCTCTCTCTTTCTCTTCGATCTCTTCTTTCTCCAACGATAACAACCTTCTCTTTCTCTCACCTACTCCTTCTATCTCTCTCCTCAATTTTCATTCTCATCTTGTGGATGCGGTGGTCAAGGAAGGGATTGAAGTTTCTCAGGAGTTTTTGGTAGGGAATTGGATTCCTCATTGCGCCGTTGCGAAGCGTGTTCCCAAGCAGAGAATGTCTGAAGCCTTTTCGCTTCTTAGAGACCTTAAATTGCCGGTTCAAGGATATGCTGTTGATGTTGCGCTCGTGCAATTCTCGCCGGTTCGTGAGATTTTCTCCTTTGTGCTTGGTAACAACAGTAATATTATAGAGAATTGATTTTATTTTATTTTATTTGAATCTTTTAGTAGCTTAATTTTGTCTGTATGTTAGTTTGTTGTAATAAGATATTTTTCTTGTTGTTGTTGTTGTTGATGATGATAATTAAGTAATTCTTGAATTGAAACTAGTTTTGGAGGAATTGTTCATGAATTATGTTGATTAGT
TARGET=748,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05121 Ps_contig_mira-and-tgicl-ass_franssen_36505
SEQUENCE=GTCGGGGATGCTTTTGAGTGAGAGAAACCGGTGAAGAGAGATATGAGTAAGTGGTGCATTTTGGTGGTTTTGGCTCTTGCTGTAGTTGCAACAAGTGCAAGAAATGTGCCTGCTGGAGAGGGTGGTTTGAAGGACGAGAAGACCTTTGGTGGGCTTGGTGGTTTTACAGGAGTTGGTAACAATGGTTTTCCATTTGGAGGTGCAGGCTTGGGCGGTGGTGGTGACGCTGGTGGTGGTGGACTTGGCGGACTAGGAGGAGTTGGTGCTGGACTCGGTGGAATAGGAGGTGCTGGACTCGGTGGAATAGGAGGTGCTGGACTGGGTGATGCTAGTGGACTAGATGGTGGTGGACTGGGTGGTTCTGCTGGACTCGGAGGATTCAGCGGGTTAGGCGGTGGTGGATTAGGTGGACTAGGTGGTTCTAATGGATTGGATGGAGTCAGCGGACTTGGTGGTGGCGGACTGGGAGGTTCTGGTGGTGGTGTCTTTCCTCATCCTTGAGTAACAAGACAATTACTATATATGGAATTTACTTTATTTTACTATTATTAAGAAAATAAAAGTTAGTTATATTGGGGTTTATTGTTATCACAATTGTTTAGTTTTAGTGTTCTATTATTATATTTTTCACCTACAGAAACTGTGTTAGCTAGTACTATTTTGTTTTGTTACGATGTTTATGATGTGTTGTTCTTTCTAATATAATGTAATGTAATTGTAAGGGATATACATTTCATCATCTACAAGTTTATTAATTAAACTGAAAATTTATGTATATATATGAAATATTATATATTTTAAAA
TARGET=228,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3959
PRIMER_LEFT_PENALTY=0.153161
PRIMER_RIGHT_PENALTY=0.242742
PRIMER_LEFT_SEQUENCE=GTGGTTTGAAGGACGAGAAGAC
PRIMER_RIGHT_SEQUENCE=TCTAGTCCACTAGCATCACCCA
PRIMER_LEFT=121,22
PRIMER_RIGHT=340,22
PRIMER_LEFT_TM=60.153
PRIMER_RIGHT_TM=59.757
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.4020
PRIMER_LEFT_1_PENALTY=0.153161
PRIMER_RIGHT_1_PENALTY=0.248820
PRIMER_LEFT_1_SEQUENCE=GTGGTTTGAAGGACGAGAAGAC
PRIMER_RIGHT_1_SEQUENCE=CTAGTCCACCTAATCCACCACC
PRIMER_LEFT_1=121,22
PRIMER_RIGHT_1=414,22
PRIMER_LEFT_1_TM=60.153
PRIMER_RIGHT_1_TM=59.751
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=0.4020
PRIMER_LEFT_2_PENALTY=0.153161
PRIMER_RIGHT_2_PENALTY=0.248820
PRIMER_LEFT_2_SEQUENCE=GTGGTTTGAAGGACGAGAAGAC
PRIMER_RIGHT_2_SEQUENCE=CCTAGTCCACCTAATCCACCAC
PRIMER_LEFT_2=121,22
PRIMER_RIGHT_2=415,22
PRIMER_LEFT_2_TM=60.153
PRIMER_RIGHT_2_TM=59.751
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.4020
PRIMER_LEFT_3_PENALTY=0.153161
PRIMER_RIGHT_3_PENALTY=0.248820
PRIMER_LEFT_3_SEQUENCE=GTGGTTTGAAGGACGAGAAGAC
PRIMER_RIGHT_3_SEQUENCE=ACCTAGTCCACCTAATCCACCA
PRIMER_LEFT_3=121,22
PRIMER_RIGHT_3=416,22
PRIMER_LEFT_3_TM=60.153
PRIMER_RIGHT_3_TM=59.751
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.4020
PRIMER_LEFT_4_PENALTY=0.153161
PRIMER_RIGHT_4_PENALTY=0.248820
PRIMER_LEFT_4_SEQUENCE=GTGGTTTGAAGGACGAGAAGAC
PRIMER_RIGHT_4_SEQUENCE=ACCACCTAGTCCACCTAATCCA
PRIMER_LEFT_4=121,22
PRIMER_RIGHT_4=419,22
PRIMER_LEFT_4_TM=60.153
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_05122 Ps_contig_mira-and-tgicl-ass_franssen_36509
SEQUENCE=TCGGGGATCTATTCCCATTCCNACGAAACAAAACAAAGGTTGTTACATTTTTCATTAATTTATTGCCTTCTTCCTCTTCCATACATTACACACACTCACACTCTCTCTATTCCATTTATTCATTCTTCTTCTTCTTCTTCTTCTTCTTCTGTAACTATGGATCTCTGCACATTCCCATGTCCTGCTTTTATCACACCTTCTCCTCATCCTAAAACAGCACTTTCTCACTCTCTATCTCAACGGCCTCTTCATTCTCTCTCTCCCTCCATCAATCAAAAGTATGCTGGAACTCGACTTTACGCTTCACTTTCAGAGACGGGGGAGTATTACTCACAGAGACCTCCAACTCCATTACTCGACACCATTAACTATCCAATCCATATGAAAAATCTCTCAACCAAGGAACTGAAACAACTTGCGGATGAGCTTCGTTCTGATGTTATTTTCAGTGTTTCAAGAACCGGCGGCCATTTAGGTTCGAGTTTAGGTGTTGTTGAACTCACTATTGCTCTTCACTATGTCTTTAATACCCCTCAGGACAAGATTCTGTGGGATGTTGGTCACCAGTCATACCCTCATAAAATACTCACTGGTAGAAGAGATAAGATGCATACTATTAGACAGACTAATGGATTGGCTGGATTCACAAAACGATCTGAGAGTGAATATGATTCTTTTGGCACGGGTCATAGTTCAACCACTATTTCAGCTGGACTTGGTAATGNCTNCTTTCTACTATCTGCCTGTAAAAAATTATTCATCACCGACACTTCGCCGTCTGAAGGCGTGTCCTTGTGTCCGACACGTGCCACGTGCTTCCATAAATGATTAAATTCAATAACCTCCGTTTTTCAAATAATTACCGGTGTCATCGTGTCAGCGGTGCA
TARGET=123,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2138
PRIMER_LEFT_PENALTY=0.196811
PRIMER_RIGHT_PENALTY=0.016950
PRIMER_LEFT_SEQUENCE=TTATTGCCTTCTTCCTCTTCCA
PRIMER_RIGHT_SEQUENCE=GAAGAGGCCGTTGAGATAGAGA
PRIMER_LEFT=59,22
PRIMER_RIGHT=249,22
PRIMER_LEFT_TM=60.197
PRIMER_RIGHT_TM=59.983
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.2373
PRIMER_LEFT_1_PENALTY=0.196811
PRIMER_RIGHT_1_PENALTY=0.040447
PRIMER_LEFT_1_SEQUENCE=TTATTGCCTTCTTCCTCTTCCA
PRIMER_RIGHT_1_SEQUENCE=TAATACTCCCCCGTCTCTGAAA
PRIMER_LEFT_1=59,22
PRIMER_RIGHT_1=328,22
PRIMER_LEFT_1_TM=60.197
PRIMER_RIGHT_1_TM=59.960
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.2917
PRIMER_LEFT_2_PENALTY=0.274705
PRIMER_RIGHT_2_PENALTY=0.016950
PRIMER_LEFT_2_SEQUENCE=TTGCCTTCTTCCTCTTCCATAC
PRIMER_RIGHT_2_SEQUENCE=GAAGAGGCCGTTGAGATAGAGA
PRIMER_LEFT_2=62,22
PRIMER_RIGHT_2=249,22
PRIMER_LEFT_2_TM=59.725
PRIMER_RIGHT_2_TM=59.983
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.3141
PRIMER_LEFT_3_PENALTY=0.196811
PRIMER_RIGHT_3_PENALTY=0.117281
PRIMER_LEFT_3_SEQUENCE=TTATTGCCTTCTTCCTCTTCCA
PRIMER_RIGHT_3_SEQUENCE=TCGAGTTCCAGCATACTTTTGA
PRIMER_LEFT_3=59,22
PRIMER_RIGHT_3=293,22
PRIMER_LEFT_3_TM=60.197
PRIMER_RIGHT_3_TM=59.883
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.3152
PRIMER_LEFT_4_PENALTY=0.274705
PRIMER_RIGHT_4_PENALTY=0.040447
PRIMER_LEFT_4_SEQUENCE=TTGCCTTCTTCCTCTTCCATAC
PRIMER_RIGHT_4_SEQUENCE=TAATACTCCCCCGTCTCTGAAA
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=328,22
PRIMER_LEFT_4_TM=59.725
PRIMER_RIGHT_4_TM=59.960
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_05123 Ps_contig_mira-and-tgicl-ass_franssen_36509
SEQUENCE=TCGGGGATCTATTCCCATTCCNACGAAACAAAACAAAGGTTGTTACATTTTTCATTAATTTATTGCCTTCTTCCTCTTCCATACATTACACACACTCACACTCTCTCTATTCCATTTATTCATTCTTCTTCTTCTTCTTCTTCTTCTTCTGTAACTATGGATCTCTGCACATTCCCATGTCCTGCTTTTATCACACCTTCTCCTCATCCTAAAACAGCACTTTCTCACTCTCTATCTCAACGGCCTCTTCATTCTCTCTCTCCCTCCATCAATCAAAAGTATGCTGGAACTCGACTTTACGCTTCACTTTCAGAGACGGGGGAGTATTACTCACAGAGACCTCCAACTCCATTACTCGACACCATTAACTATCCAATCCATATGAAAAATCTCTCAACCAAGGAACTGAAACAACTTGCGGATGAGCTTCGTTCTGATGTTATTTTCAGTGTTTCAAGAACCGGCGGCCATTTAGGTTCGAGTTTAGGTGTTGTTGAACTCACTATTGCTCTTCACTATGTCTTTAATACCCCTCAGGACAAGATTCTGTGGGATGTTGGTCACCAGTCATACCCTCATAAAATACTCACTGGTAGAAGAGATAAGATGCATACTATTAGACAGACTAATGGATTGGCTGGATTCACAAAACGATCTGAGAGTGAATATGATTCTTTTGGCACGGGTCATAGTTCAACCACTATTTCAGCTGGACTTGGTAATGNCTNCTTTCTACTATCTGCCTGTAAAAAATTATTCATCACCGACACTTCGCCGTCTGAAGGCGTGTCCTTGTGTCCGACACGTGCCACGTGCTTCCATAAATGATTAAATTCAATAACCTCCGTTTTTCAAATAATTACCGGTGTCATCGTGTCAGCGGTGCA
TARGET=253,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0574
PRIMER_LEFT_PENALTY=0.016950
PRIMER_RIGHT_PENALTY=0.040447
PRIMER_LEFT_SEQUENCE=TCTCTATCTCAACGGCCTCTTC
PRIMER_RIGHT_SEQUENCE=TAATACTCCCCCGTCTCTGAAA
PRIMER_LEFT=228,22
PRIMER_RIGHT=328,22
PRIMER_LEFT_TM=59.983
PRIMER_RIGHT_TM=59.960
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=0.2373
PRIMER_LEFT_1_PENALTY=0.196811
PRIMER_RIGHT_1_PENALTY=0.040447
PRIMER_LEFT_1_SEQUENCE=TTATTGCCTTCTTCCTCTTCCA
PRIMER_RIGHT_1_SEQUENCE=TAATACTCCCCCGTCTCTGAAA
PRIMER_LEFT_1=59,22
PRIMER_RIGHT_1=328,22
PRIMER_LEFT_1_TM=60.197
PRIMER_RIGHT_1_TM=59.960
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.2436
PRIMER_LEFT_2_PENALTY=0.016950
PRIMER_RIGHT_2_PENALTY=0.226618
PRIMER_LEFT_2_SEQUENCE=TCTCTATCTCAACGGCCTCTTC
PRIMER_RIGHT_2_SEQUENCE=GGAGTTGGAGGTCTCTGTGAGT
PRIMER_LEFT_2=228,22
PRIMER_RIGHT_2=349,22
PRIMER_LEFT_2_TM=59.983
PRIMER_RIGHT_2_TM=59.773
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=0.2444
PRIMER_LEFT_3_PENALTY=0.203971
PRIMER_RIGHT_3_PENALTY=0.040447
PRIMER_LEFT_3_SEQUENCE=CATTCCCATGTCCTGCTTTTAT
PRIMER_RIGHT_3_SEQUENCE=TAATACTCCCCCGTCTCTGAAA
PRIMER_LEFT_3=169,22
PRIMER_RIGHT_3=328,22
PRIMER_LEFT_3_TM=60.204
PRIMER_RIGHT_3_TM=59.960
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=0.2657
PRIMER_LEFT_4_PENALTY=0.016950
PRIMER_RIGHT_4_PENALTY=0.248781
PRIMER_LEFT_4_SEQUENCE=TCTCTATCTCAACGGCCTCTTC
PRIMER_RIGHT_4_SEQUENCE=TGTTTCAGTTCCTTGGTTGAGA
PRIMER_LEFT_4=228,22
PRIMER_RIGHT_4=412,22
PRIMER_LEFT_4_TM=59.983
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_05124 Ps_contig_mira-and-tgicl-ass_franssen_36510
SEQUENCE=AAGCAAGTGGTAGTCAACGCAGATACGGGACACTCACACTCTCCTCTTAAGCCCTAGTAGCTAATTAACTAGTATTTGCAACAAAACACTAGCTATATTACTACAAAGAGTTTACCTAAAATGGCTTCCAAAACTTGTTCCTCCCTTGCAATTTTCCTCACAATCAACATTCTCTTCTTTACACTTGTTTCATCTTGTGGAACTTGCGGCTCCGGCCCATACCCAAACCCAAACCCTAAGCCAAAGCATACTCCAAGCCCAAACCCCTCAGGTGGCTCGAGTCATTCTGGTGGCTCGAGTCCGGGAGGCTCTAGTCCTTCAGGAGGTTCATATCCCTCAGGTCCTTCCGGCCCTTCAGGCGGGGGTAGCACCGTCTCGTGCCCGCGTGATGCACTCAAACTCGGCGTATGTGCCAATGTCCTAAATGGTTTGCTGAATCTAACTTTGGGCCAGCCACCAGTGACACCATGTTGCTCCCTTCTAAATGGCCTTGTTGATCTTGAGGCTGCAGTGTGTCTTTGCACTGCCCTTAGAGCTAACATTTTGGGGATAAATCTCAACCTTCCTATCTCACTTAGCTTACTTCTCAATGTTTGTTCAAAAGAAGCACCACGTGATTTCCAATGCTACTAATTAGTTCTTCATCATCATCACCATCAAGATCATATCAACAAAGTATCTACTTTATTTGCTTATTCGTTTTAAAGTTTCGTTGTTTATTCTAAATTATCCATTTGATCTCTCAGTTTGAGAGATTTGTTCATTGCATGGGAGATTAACYTNNCCTCTCGTTATATCTATTCAGTTGGTATTCTAGTTTC
TARGET=642,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05125 Ps_contig_mira-and-tgicl-ass_franssen_36516
SEQUENCE=ACCCATGAGATGATATGAAGAAACAAAAGTTCTGACAGTGGCTTTGCAGTGACACTGAAAGAGAAAAAAAAATTAGTTGATTCATTCATTCAATTCATCTTCTTCTTCACAATTCACCGGAAAAATTCGATGAAGCTGAAAGAGTTAGAAGGTCACCTAGGTTCTCTTGATCAATTTCCTAACCCTAAGATTGAGCTTGAGCAATACCCAACAGGACCACACATCGCTTCTCGCATGCTTTTTCTTGCAGAGAATTCTTATGACGATGTGAGCAACAAAGTTGTGGCTGATTTTGGATGTGGTTGTGGTACTTTAGGCATTGCAGCTGCCCTTTTGAGCGCAGAACATGTTCTCAGCATTGACATTGATCCAGAATCTCTTGAAATAGCATCTCTTAATGCTGAGGAACTCGAGCTGGACATGGATTTTATTCAATCTAATATCATGGACTTGAGATGGAGAGGTCCAATTGTCGATACAGTTATAATGAATCCTCCATTTGGAACTCGGAAAAAGGGTGCAGATTTGGAGTTTCTTTCTGCTGCTATGAAGGTTGCTTCTCAAGCTGTTTATTCTTTGCATAAAACTTCAACAAGAGATCATGTGAAACGTGCAGCCTTAAGGGATTTCAATGCTAGAAGTGCTGAAGTTCTATGTGAGCTTCGGTATGATGTGCCGAAATTGTACAAATTTCATAAGAAGAAAGAGGTCGATATTGCTGTTGATCTCTGGCGTTTTGAACCTGCAAATCATCAGAGCTGAGGCATTTAGTGCAGACAGATTGCAATAGATGATAAAGTTGAAAATTAGAGATGAAATTTTGTTATGTACCTATATAAGAAAGATATTGTGTTTTTCCTCTCTGTTACAAGCTCTTTGATAGACTTGGGAGTTTCCTCTTCGGTAACTTGTTTGAACTTTGGAATTCCTTTTCAAATTTATTCTAGTGATTCAGG
TARGET=80,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0171
PRIMER_LEFT_PENALTY=0.830836
PRIMER_RIGHT_PENALTY=0.186309
PRIMER_LEFT_SEQUENCE=AAAGTTCTGACAGTGGCTTTGC
PRIMER_RIGHT_SEQUENCE=CTGCAATGCCTAAAGTACCACA
PRIMER_LEFT=25,22
PRIMER_RIGHT=324,22
PRIMER_LEFT_TM=60.831
PRIMER_RIGHT_TM=60.186
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=1.0786
PRIMER_LEFT_1_PENALTY=0.830836
PRIMER_RIGHT_1_PENALTY=0.247716
PRIMER_LEFT_1_SEQUENCE=AAAGTTCTGACAGTGGCTTTGC
PRIMER_RIGHT_1_SEQUENCE=GGGTATTGCTCAAGCTCAATCT
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=208,22
PRIMER_LEFT_1_TM=60.831
PRIMER_RIGHT_1_TM=59.752
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=1.1228
PRIMER_LEFT_2_PENALTY=0.830836
PRIMER_RIGHT_2_PENALTY=0.291979
PRIMER_LEFT_2_SEQUENCE=AAAGTTCTGACAGTGGCTTTGC
PRIMER_RIGHT_2_SEQUENCE=ACAACTTTGTTGCTCACATCGT
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=283,22
PRIMER_LEFT_2_TM=60.831
PRIMER_RIGHT_2_TM=59.708
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=8.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=1.1631
PRIMER_LEFT_3_PENALTY=0.830836
PRIMER_RIGHT_3_PENALTY=0.332305
PRIMER_LEFT_3_SEQUENCE=AAAGTTCTGACAGTGGCTTTGC
PRIMER_RIGHT_3_SEQUENCE=ACTTTGTTGCTCACATCGTCAT
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=280,22
PRIMER_LEFT_3_TM=60.831
PRIMER_RIGHT_3_TM=59.668
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=1.2235
PRIMER_LEFT_4_PENALTY=0.830836
PRIMER_RIGHT_4_PENALTY=0.392624
PRIMER_LEFT_4_SEQUENCE=AAAGTTCTGACAGTGGCTTTGC
PRIMER_RIGHT_4_SEQUENCE=TGCTCAAGCTCAATCTTAGGGT
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=202,22
PRIMER_LEFT_4_TM=60.831
PRIMER_RIGHT_4_TM=60.393
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=178
=
PRIMER_SEQUENCE_ID=SSR_05126 Ps_contig_mira-and-tgicl-ass_franssen_36518
SEQUENCE=AAAGAACAAGTAGGTAATCAAACGCAGAGTACGCGGGACTGACTCTAACAAACCTACTTTCTCTCTCTTCTCTCTCTCTCTTCAATGGGTTGTGCTGGCTCATCTCAGTCCAAGCCTGATGGGACTGTAAAAAAGATCCGAAAGCCTAAGCCTTGGAAGCATCCTCAGCCCATAACAAGGACTCAGCTAATACAATTGCGCGATGAGTTTTGGGACACAGCTCCTCACTATGGTGGACGGAAAGAGATTTGGGATGCTCTTCGAGCTGCTGCAGAAGCTGATCTTTCTTTAGCACAGGCAATTGTGGATAGTGCTGGGGTCATTGTCCAGAGTTCTGATTTGACAGTATGCTATGATGAAAGAGGTGCGAAGTACGAGCTACCCAAGTATGTTTTGAGTGAGCCAACTAACTTGATTCGAGAAAGTTAATAACAAGGAGTAAATATAGTAACATTCATGGGAAGAAGCATATTGTAAATCCTGTATATTTTTATCTCATTGATTTGGTTGGTAACTTTTATAGGGTAGAAACTTTTTTCCATATCCAACTGTACTACTCACCTCACCCTTGACTTTATGTTTCCTCAATCAATGGAACAAAAATTATGTCTGTTCTGTATCAAATGATGATAAGTATCATCACTCTTTGGCAAATACAAACTCAATTGTGTATCTTCCTTCCCAAATGAGTAAAGTGCAATAAGACAATTACTACGAAAANANNGACTTTGTTTGCATTTCATAAAAAAAAAGACTTTGCC
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1628
PRIMER_LEFT_PENALTY=0.162046
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=AGTACGCGGGACTGACTCTAAC
PRIMER_RIGHT_SEQUENCE=TTTTTACAGTCCCATCAGGCTT
PRIMER_LEFT=27,22
PRIMER_RIGHT=132,22
PRIMER_LEFT_TM=59.838
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=0.1799
PRIMER_LEFT_1_PENALTY=0.162046
PRIMER_RIGHT_1_PENALTY=0.017846
PRIMER_LEFT_1_SEQUENCE=AGTACGCGGGACTGACTCTAAC
PRIMER_RIGHT_1_SEQUENCE=TGCCTGTGCTAAAGAAAGATCA
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=299,22
PRIMER_LEFT_1_TM=59.838
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.3140
PRIMER_LEFT_2_PENALTY=0.162046
PRIMER_RIGHT_2_PENALTY=0.151911
PRIMER_LEFT_2_SEQUENCE=AGTACGCGGGACTGACTCTAAC
PRIMER_RIGHT_2_SEQUENCE=CTATCCACAATTGCCTGTGCTA
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=310,22
PRIMER_LEFT_2_TM=59.838
PRIMER_RIGHT_2_TM=60.152
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=284
PRIMER_PAIR_PENALTY_3=0.3267
PRIMER_LEFT_3_PENALTY=0.162046
PRIMER_RIGHT_3_PENALTY=0.164639
PRIMER_LEFT_3_SEQUENCE=AGTACGCGGGACTGACTCTAAC
PRIMER_RIGHT_3_SEQUENCE=AAATCTCTTTCCGTCCACCATA
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=59.838
PRIMER_RIGHT_3_TM=59.835
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.3411
PRIMER_LEFT_4_PENALTY=0.323242
PRIMER_RIGHT_4_PENALTY=0.017846
PRIMER_LEFT_4_SEQUENCE=CGGGACTGACTCTAACAAACCT
PRIMER_RIGHT_4_SEQUENCE=TGCCTGTGCTAAAGAAAGATCA
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=299,22
PRIMER_LEFT_4_TM=59.677
PRIMER_RIGHT_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_05127 Ps_contig_mira-and-tgicl-ass_franssen_36523
SEQUENCE=AGCAGGGACCATGACCACATCTCTCGGAATTTCGCATATTCACACACACAACAGAACGAAACATAAAAAACACGATATTCATAACAAAATCGTAATTTACTTCTCCATCAAGATTCTAATTATCGTCTTTCTTTCTGTATCCCTTATTAACCTATTTAATTATTTTATTAATCCTCTAATCTATCTGAGAAATTGATTGTTATGGGCGAGTTGACTCAGACAGAGGTGTACTCGCCGCCGCGAACTCAGCAAGTTTGGAAATCACTTTTAAGCTGGTTCACGTTTTTCTTTCAGATCTTTTCTCAGATTATAAGAACCCTAGGTCACTATCCTTTACTCTCTTCTTCATCTTCTTCTTCTTCGTCTTCTTCTTCAACTTCATCTTCCTTCAAACCTCTTCCTTCTGTTGAGCTTCAGGAATACGATTCGTCAACGACTTCCGCCGTTGAAATCACCGCCGATTTTCCCGTCTCCGATCACCGTCCCGTCCAAAAACTCACGGTAGTGCTTGACTTGGATGAAACACTAGTATGCGCATATGAGACATCAAGTTTGCCAACTGCACTACGTTCTCAAGCAATCGAAGCTGGTTTGAATTGGTTTGAGCTGGAGTGTGTATCTTCTGACAAGGAAGGAGAAGGCAAACCAAAAATCAATTATGTTACAGTCTTTGAACGCCCTGGGTTGAAGGAATTCTTAACAAAACTTAGTGAATTTGCTGACTTGGTGCTGTTTACTGCTGGTCTCGAAGGCTATGCTAGACCCCTTGTTGACATAATAGACAAGAAAAATCTGTTCAGTCTACGACTTTATCGGCCATCAACAATTAGCACGGAATATAGGGAACATGTTAAGGATCTTACATTCATATCAAAGGATCTAGACCGCATTGTTATTGTAGACAACAATCCCTTCAGCTTTGTATTACAACCAGTTAATGGAATTCCATGTATTCCATTTTCTGCCGGGCATCCACATGACACCCAGCTTCTAGATGTCATTCTTCCACTTCTCAAGCAACTCTCTGAGCAGAATGATGTAAGACCTTTGCTCTATGAAAAATTCCACATGACTGATTGGTTCCAGAAGCAAGGGATTCCAGCTTCCAGTTGGAAATTGTAGGATTAAAATTGTTCGCCTCTAGGCTCTTTTGCTTTTCTCGGGTGAAGTTCTTAGTTATATTGCTGGAAAGTGCAGCTGCTGATGGGATGATATAGTATTTTTTGTATAGCACCTAATTTATTACT
TARGET=41,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5478
PRIMER_LEFT_PENALTY=0.167242
PRIMER_RIGHT_PENALTY=0.380537
PRIMER_LEFT_SEQUENCE=ATGACCACATCTCTCGGAATTT
PRIMER_RIGHT_SEQUENCE=AGTCAACTCGCCCATAACAATC
PRIMER_LEFT=10,22
PRIMER_RIGHT=215,22
PRIMER_LEFT_TM=59.833
PRIMER_RIGHT_TM=60.381
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.5478
PRIMER_LEFT_1_PENALTY=0.167242
PRIMER_RIGHT_1_PENALTY=0.380537
PRIMER_LEFT_1_SEQUENCE=ATGACCACATCTCTCGGAATTT
PRIMER_RIGHT_1_SEQUENCE=GAGTCAACTCGCCCATAACAAT
PRIMER_LEFT_1=10,22
PRIMER_RIGHT_1=216,22
PRIMER_LEFT_1_TM=59.833
PRIMER_RIGHT_1_TM=60.381
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.8399
PRIMER_LEFT_2_PENALTY=0.167242
PRIMER_RIGHT_2_PENALTY=0.672685
PRIMER_LEFT_2_SEQUENCE=ATGACCACATCTCTCGGAATTT
PRIMER_RIGHT_2_SEQUENCE=CGCCCATAACAATCAATTTCTC
PRIMER_LEFT_2=10,22
PRIMER_RIGHT_2=207,22
PRIMER_LEFT_2_TM=59.833
PRIMER_RIGHT_2_TM=60.673
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=1.1902
PRIMER_LEFT_3_PENALTY=0.167242
PRIMER_RIGHT_3_PENALTY=1.022919
PRIMER_LEFT_3_SEQUENCE=ATGACCACATCTCTCGGAATTT
PRIMER_RIGHT_3_SEQUENCE=ACCTCTGTCTGAGTCAACTCGC
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=226,22
PRIMER_LEFT_3_TM=59.833
PRIMER_RIGHT_3_TM=61.023
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=1.2161
PRIMER_LEFT_4_PENALTY=0.167242
PRIMER_RIGHT_4_PENALTY=1.048901
PRIMER_LEFT_4_SEQUENCE=ATGACCACATCTCTCGGAATTT
PRIMER_RIGHT_4_SEQUENCE=ACGTGAACCAGCTTAAAAGTGA
PRIMER_LEFT_4=10,22
PRIMER_RIGHT_4=282,22
PRIMER_LEFT_4_TM=59.833
PRIMER_RIGHT_4_TM=58.951
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_05128 Ps_contig_mira-and-tgicl-ass_franssen_36523
SEQUENCE=AGCAGGGACCATGACCACATCTCTCGGAATTTCGCATATTCACACACACAACAGAACGAAACATAAAAAACACGATATTCATAACAAAATCGTAATTTACTTCTCCATCAAGATTCTAATTATCGTCTTTCTTTCTGTATCCCTTATTAACCTATTTAATTATTTTATTAATCCTCTAATCTATCTGAGAAATTGATTGTTATGGGCGAGTTGACTCAGACAGAGGTGTACTCGCCGCCGCGAACTCAGCAAGTTTGGAAATCACTTTTAAGCTGGTTCACGTTTTTCTTTCAGATCTTTTCTCAGATTATAAGAACCCTAGGTCACTATCCTTTACTCTCTTCTTCATCTTCTTCTTCTTCGTCTTCTTCTTCAACTTCATCTTCCTTCAAACCTCTTCCTTCTGTTGAGCTTCAGGAATACGATTCGTCAACGACTTCCGCCGTTGAAATCACCGCCGATTTTCCCGTCTCCGATCACCGTCCCGTCCAAAAACTCACGGTAGTGCTTGACTTGGATGAAACACTAGTATGCGCATATGAGACATCAAGTTTGCCAACTGCACTACGTTCTCAAGCAATCGAAGCTGGTTTGAATTGGTTTGAGCTGGAGTGTGTATCTTCTGACAAGGAAGGAGAAGGCAAACCAAAAATCAATTATGTTACAGTCTTTGAACGCCCTGGGTTGAAGGAATTCTTAACAAAACTTAGTGAATTTGCTGACTTGGTGCTGTTTACTGCTGGTCTCGAAGGCTATGCTAGACCCCTTGTTGACATAATAGACAAGAAAAATCTGTTCAGTCTACGACTTTATCGGCCATCAACAATTAGCACGGAATATAGGGAACATGTTAAGGATCTTACATTCATATCAAAGGATCTAGACCGCATTGTTATTGTAGACAACAATCCCTTCAGCTTTGTATTACAACCAGTTAATGGAATTCCATGTATTCCATTTTCTGCCGGGCATCCACATGACACCCAGCTTCTAGATGTCATTCTTCCACTTCTCAAGCAACTCTCTGAGCAGAATGATGTAAGACCTTTGCTCTATGAAAAATTCCACATGACTGATTGGTTCCAGAAGCAAGGGATTCCAGCTTCCAGTTGGAAATTGTAGGATTAAAATTGTTCGCCTCTAGGCTCTTTTGCTTTTCTCGGGTGAAGTTCTTAGTTATATTGCTGGAAAGTGCAGCTGCTGATGGGATGATATAGTATTTTTTGTATAGCACCTAATTTATTACT
TARGET=349,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2027
PRIMER_LEFT_PENALTY=0.088247
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=CGAGTTGACTCAGACAGAGGTG
PRIMER_RIGHT_SEQUENCE=ATTCCTGAAGCTCAACAGAAGG
PRIMER_LEFT=206,22
PRIMER_RIGHT=420,22
PRIMER_LEFT_TM=60.088
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=215
PRIMER_PAIR_PENALTY_1=0.2304
PRIMER_LEFT_1_PENALTY=0.088247
PRIMER_RIGHT_1_PENALTY=0.142170
PRIMER_LEFT_1_SEQUENCE=CGAGTTGACTCAGACAGAGGTG
PRIMER_RIGHT_1_SEQUENCE=CAGAAGGAAGAGGTTTGAAGGA
PRIMER_LEFT_1=206,22
PRIMER_RIGHT_1=405,22
PRIMER_LEFT_1_TM=60.088
PRIMER_RIGHT_1_TM=59.858
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.3100
PRIMER_LEFT_2_PENALTY=0.195574
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=AGCTGGTTCACGTTTTTCTTTC
PRIMER_RIGHT_2_SEQUENCE=ATTCCTGAAGCTCAACAGAAGG
PRIMER_LEFT_2=270,22
PRIMER_RIGHT_2=420,22
PRIMER_LEFT_2_TM=59.804
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=151
PRIMER_PAIR_PENALTY_3=0.3360
PRIMER_LEFT_3_PENALTY=0.088247
PRIMER_RIGHT_3_PENALTY=0.247716
PRIMER_LEFT_3_SEQUENCE=CGAGTTGACTCAGACAGAGGTG
PRIMER_RIGHT_3_SEQUENCE=ACGAATCGTATTCCTGAAGCTC
PRIMER_LEFT_3=206,22
PRIMER_RIGHT_3=429,22
PRIMER_LEFT_3_TM=60.088
PRIMER_RIGHT_3_TM=59.752
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.3548
PRIMER_LEFT_4_PENALTY=0.195574
PRIMER_RIGHT_4_PENALTY=0.159203
PRIMER_LEFT_4_SEQUENCE=AGCTGGTTCACGTTTTTCTTTC
PRIMER_RIGHT_4_SEQUENCE=TGCGCATACTAGTGTTTCATCC
PRIMER_LEFT_4=270,22
PRIMER_RIGHT_4=536,22
PRIMER_LEFT_4_TM=59.804
PRIMER_RIGHT_4_TM=60.159
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_05129 Ps_contig_mira-and-tgicl-ass_franssen_36534
SEQUENCE=TTTGGAACCTGAATGGTTAACGANCTTGGTATGCAGGCGCAATGGCGTTTGATGCAGAATATGCACGATGGTTAGAAGAGCATAATAGGCAAACCAATGAACTAAGGGCTGCAATAAATTCTCATGCGGGGGATATTGAACTCCGTACCATTGTGGACAATTTCATGACTCAATTTGAAGATATCTACAGGCTGAAAGGTGTTGCAGCAAAAGCTGATGTTTTCCACATTCTATCAGGAATGTGGAAGACTCCCGCTGAGAGGTGTTTCATGTGGATTGGAGGCTTTCGGTCATCCGAACTTCTCAAGCTTCTTGTAAGTCATTTGGAGCCTTTAACCGAGCAACAATTAATGGGCATCTACAACTTGCAACAATCATCTCAACAGGCGGAAGATGCTCTCTCCCAAGGAATGGATGCTTTGCAGCAATCACTTTCCGAGACTTTGGCTAATGGTTCGCCCAACCCTTCGGGTTCTTCTGGAAATGTGGCTAACTACATGGGCCAAATGGCCATGGCCATGGGAAAGCTCGGTACTCTCGAGGGCTTTCTTCGCCAGGCTGATAATCTGCGGCAGCAAACATTGCAACAGATGCTTCGGATATTAACAACCAGACAATCAGCTCGAGCTCTTCTTGCAATAAGCGATTATTTCTCCCGGCTACGAGCCTTGAGTTCTCTATGGCTTGCGCGACCAAGGGAGTAAAAAACTGTTTCAAACCTCTATTTATGTTTCATTTGGCAAATGAATTCCTATTGTGAAGAAGAAGAAGAAGCCTAGTTGTGGTGCTTGTAAAAACATTAGATACTATGATTTTTGTATTGTCATCAACATTTTTCATTAAGCTTTTTGTATGCTTTGATAAATAGTGTTGAATAATTTGATTATATAGGTGTATGATAGATAGAGTTGAAGGTGAAAAATGTAGAAATAGGTTAAAGTTGTTCATTGTATTTCAGAAATCAAGCTTATTTGAGGATGATTTCCTCTCTTTTAGTTTCTTTTCTTGCTGGTTGGTTGCACAGTGGTGTATGTATGTATGTAAATATACTTTTTTTTTTGGTGCTGTATTGGGATGATATTGTTGAATAGTAATTTAATGTGTTTCAGTGCC
TARGET=759,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.1036
PRIMER_LEFT_PENALTY=0.304841
PRIMER_RIGHT_PENALTY=2.798787
PRIMER_LEFT_SEQUENCE=TAACAACCAGACAATCAGCTCG
PRIMER_RIGHT_SEQUENCE=GTTTTTACAAGCACCACAACTAGG
PRIMER_LEFT=603,22
PRIMER_RIGHT=797,24
PRIMER_LEFT_TM=60.305
PRIMER_RIGHT_TM=59.201
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=3.1914
PRIMER_LEFT_1_PENALTY=0.392624
PRIMER_RIGHT_1_PENALTY=2.798787
PRIMER_LEFT_1_SEQUENCE=CCTTGAGTTCTCTATGGCTTGC
PRIMER_RIGHT_1_SEQUENCE=GTTTTTACAAGCACCACAACTAGG
PRIMER_LEFT_1=666,22
PRIMER_RIGHT_1=797,24
PRIMER_LEFT_1_TM=60.393
PRIMER_RIGHT_1_TM=59.201
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=3.1914
PRIMER_LEFT_2_PENALTY=0.392624
PRIMER_RIGHT_2_PENALTY=2.798787
PRIMER_LEFT_2_SEQUENCE=GCCTTGAGTTCTCTATGGCTTG
PRIMER_RIGHT_2_SEQUENCE=GTTTTTACAAGCACCACAACTAGG
PRIMER_LEFT_2=665,22
PRIMER_RIGHT_2=797,24
PRIMER_LEFT_2_TM=60.393
PRIMER_RIGHT_2_TM=59.201
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=133
PRIMER_PAIR_PENALTY_3=3.2205
PRIMER_LEFT_3_PENALTY=0.304841
PRIMER_RIGHT_3_PENALTY=2.915671
PRIMER_LEFT_3_SEQUENCE=TAACAACCAGACAATCAGCTCG
PRIMER_RIGHT_3_SEQUENCE=TTTTACAAGCACCACAACTAGG
PRIMER_LEFT_3=603,22
PRIMER_RIGHT_3=795,22
PRIMER_LEFT_3_TM=60.305
PRIMER_RIGHT_3_TM=57.084
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=3.3083
PRIMER_LEFT_4_PENALTY=0.392624
PRIMER_RIGHT_4_PENALTY=2.915671
PRIMER_LEFT_4_SEQUENCE=CCTTGAGTTCTCTATGGCTTGC
PRIMER_RIGHT_4_SEQUENCE=TTTTACAAGCACCACAACTAGG
PRIMER_LEFT_4=666,22
PRIMER_RIGHT_4=795,22
PRIMER_LEFT_4_TM=60.393
PRIMER_RIGHT_4_TM=57.084
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_05130 Ps_contig_mira-and-tgicl-ass_franssen_36534
SEQUENCE=TTTGGAACCTGAATGGTTAACGANCTTGGTATGCAGGCGCAATGGCGTTTGATGCAGAATATGCACGATGGTTAGAAGAGCATAATAGGCAAACCAATGAACTAAGGGCTGCAATAAATTCTCATGCGGGGGATATTGAACTCCGTACCATTGTGGACAATTTCATGACTCAATTTGAAGATATCTACAGGCTGAAAGGTGTTGCAGCAAAAGCTGATGTTTTCCACATTCTATCAGGAATGTGGAAGACTCCCGCTGAGAGGTGTTTCATGTGGATTGGAGGCTTTCGGTCATCCGAACTTCTCAAGCTTCTTGTAAGTCATTTGGAGCCTTTAACCGAGCAACAATTAATGGGCATCTACAACTTGCAACAATCATCTCAACAGGCGGAAGATGCTCTCTCCCAAGGAATGGATGCTTTGCAGCAATCACTTTCCGAGACTTTGGCTAATGGTTCGCCCAACCCTTCGGGTTCTTCTGGAAATGTGGCTAACTACATGGGCCAAATGGCCATGGCCATGGGAAAGCTCGGTACTCTCGAGGGCTTTCTTCGCCAGGCTGATAATCTGCGGCAGCAAACATTGCAACAGATGCTTCGGATATTAACAACCAGACAATCAGCTCGAGCTCTTCTTGCAATAAGCGATTATTTCTCCCGGCTACGAGCCTTGAGTTCTCTATGGCTTGCGCGACCAAGGGAGTAAAAAACTGTTTCAAACCTCTATTTATGTTTCATTTGGCAAATGAATTCCTATTGTGAAGAAGAAGAAGAAGCCTAGTTGTGGTGCTTGTAAAAACATTAGATACTATGATTTTTGTATTGTCATCAACATTTTTCATTAAGCTTTTTGTATGCTTTGATAAATAGTGTTGAATAATTTGATTATATAGGTGTATGATAGATAGAGTTGAAGGTGAAAAATGTAGAAATAGGTTAAAGTTGTTCATTGTATTTCAGAAATCAAGCTTATTTGAGGATGATTTCCTCTCTTTTAGTTTCTTTTCTTGCTGGTTGGTTGCACAGTGGTGTATGTATGTATGTAAATATACTTTTTTTTTTGGTGCTGTATTGGGATGATATTGTTGAATAGTAATTTAATGTGTTTCAGTGCC
TARGET=1028,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05131 Ps_contig_mira-and-tgicl-ass_franssen_36540
SEQUENCE=GGTAGTNAACGCNGAGTACGCGGGGATGTCCATCCACTCGTATCCTTTTCCACCATTTCTTCTCTCTCTCTCTCTACCCCCTTTCTTCACTATAGAAACTACTACTACCAAAGTTGTCACTTTTTGCCATTGGACAAGTATATTATAATACATTATATTATATACAAATTACTTTCTTCATCCTTTCTACCCTCCATTCCTCCAACTTCATGCATGCTCTTTTTTTTCAAAAAGGAGGAAGCTTTTCCTTTCACTCTTACTTTATCTGGTTTTTGATTTGATTTTCTCATACCCTTTTGCTTCACAGGACTTCAAAAAAATGGCAGAAAGTACCTTGTTCTGTCTGTAGAATTTGGTAGAGAAAGTTAAATACCTTTGAGTCAGTCTTTTAGAATGCCAGTTCCACTTGCCCCTTACCCAACTCCACCACCAGCTCCATATACACCAACTCCAACAAATGGTTCACAGAGTCAACTTGTGTGTTCTGGTTGTAGAAACCTTCTGGTCTATCCAGTTGGAGCTACTTCTGTATGCTGTGCTGTTTGCAATGCTGTCACAGCAGTGCCACCTCCTGGCACAGAAATGGCTCAGATAGTTTGTGGAGGATGCCATACTCTACTCATGTACATCCGCGGAGCGACCAGCGTTCAATGCTCTTGTTGTCACACAGTCAATCTAGCTTTGGAAGCAAATCAGGTGGCACATGTCAATTGTGGTAACTGCAGGATGCTTCTTATGTACCAATATGGAGCAAGATCCGTGAAATGTGCAGTTTGCAATTTTGTTACCTCAGTTGGGGCTTCAGCAAGCACCACTGAGCAGAAGTTCAGCACCTAAGAAAATCATATACTATAATAGCAAGGTCATTACAGACTCTACAACCTTGGTTTTTGCTACTTACATATCAATCTTAAGGAATAATAACAAAGAGTGTCAATCAGATTGAAAACTTTCTTCACATGTATAGAGTTATTTTTCTCTTGCGTGACGACGGTGATTGTATTGAATATTTTTGTTAGAATTGGTGTATGAATAAGTGAATAACAATCTGTTCTAAGATTCTTGATTTTGAGTGCTAGTAAAAAATCCATATCTTTGGATAAGGTAAAACTTATTCTCTACTCAAGTGGTTRAGAAAG
TARGET=61,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05132 Ps_contig_mira-and-tgicl-ass_franssen_36545
SEQUENCE=CCTATCAACAATTTCATCCCATTCCTTTGTCACTCCGTCGTGATTCCAATTACAATGCTTCTTCTTCTTCCTCCTCCGTTCACAGACACAAGCACAGTTTCATAGTAGCCTCCAAAAGCAAAAAGCACCGTCACAATGACGCTAAGGGTTCCAATTCTCAACCAAGTCCTCCCAGAATCACTTCAAACGCCAAGCAAAACCTTCAAATCCTCAAGACATGGAAGGAGTACGAAAAACGAAAATCTACCGCTCCAAGACCTTCCACCAGTTACCGCAAAAAGAAGGCGGAAAAGGAGAAAAATCACGATCGTGATCCTATCTCCTCCATCGACAACTCAAACCAGTTTGTTATTATAGATGATGGTGTTCCTGTGATGCTTGTGGATGGCTATAATGTTTGTGGATATTGGATGAAGCTCAAGAAATATTTTATGAGTGGAAGACTTGAGCTGGCTCGCCAAATGCTGATTGATGAGCTTAAAACCTTTAGTATGATCAGAGAGATCAAAGTGGTTGTTGTATTTGATGCCATGATGTCTGGGTTCCCCAATCACAAGGAACGTTCTGCTGGTATCGACATTGTTTTCTCAAGTAAAACATCTGCTGATATATGGATTGAAAAAGAGGTTTCAGCTTTAAAGAAGGACGGTTGCCCCAAAGTCTGGGTTGTCACTTCTGATCGCTGTCATCGGGAAGCAGCATATGGAGAAGGAGCCTTTGTTTGGAGTTGCAAGACATTGGTAACTGAGATTGAGGAATCACATAGGGAGCTTGGTGAGATGCTTCAAGAGCAAAGATCAACTTCTTTTCAAGGTTCATTGTTGAAGCACAGTCTCAGTACAGAAGTATTGGATGCTCTTAGGAACCTGAGGCAAAAGTTATCTGAAAATGAGTCGAAGTAGGATATCAAATTAAAATTCTTGTAAGAGAATTTAGTTTGACAGGTGAATAGGTTTTAATAATCCGATGTTAAAGATGCTTTTTTTTGGCATGGATGCATTTACTATTTACTTGAAACCCTTCACTTGTTTGCTGGTTACTTTTATTCTTTCACATTATGTCAAAATCCGAACTTATTGAAAATGGCTCTGGCTGTGTCAACCATAACCGAGATATTATAAACACTGGTGTCATTTGATATCAAGACAAACAGCCTTAGGAACTATAATGTGTAAATAATCAGAATATCTGTACAGAGACAGTTTCTATAGGTTTTTGCTTGCGGGAATACAAAATCGCTGTACAATGTATTGCAAT
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7239
PRIMER_LEFT_PENALTY=0.723171
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=TTCATCCCATTCCTTTGTCACT
PRIMER_RIGHT_SEQUENCE=TCGTTTTTCGTACTCCTTCCAT
PRIMER_LEFT=12,22
PRIMER_RIGHT=238,22
PRIMER_LEFT_TM=60.723
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.7467
PRIMER_LEFT_1_PENALTY=0.723171
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=TTCATCCCATTCCTTTGTCACT
PRIMER_RIGHT_1_SEQUENCE=AGGACTTGGTTGAGAATTGGAA
PRIMER_LEFT_1=12,22
PRIMER_RIGHT_1=169,22
PRIMER_LEFT_1_TM=60.723
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=0.7830
PRIMER_LEFT_2_PENALTY=0.723171
PRIMER_RIGHT_2_PENALTY=0.059875
PRIMER_LEFT_2_SEQUENCE=TTCATCCCATTCCTTTGTCACT
PRIMER_RIGHT_2_SEQUENCE=TCCTTCCATGTCTTGAGGATTT
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=225,22
PRIMER_LEFT_2_TM=60.723
PRIMER_RIGHT_2_TM=59.940
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.8321
PRIMER_LEFT_3_PENALTY=0.723171
PRIMER_RIGHT_3_PENALTY=0.108929
PRIMER_LEFT_3_SEQUENCE=TTCATCCCATTCCTTTGTCACT
PRIMER_RIGHT_3_SEQUENCE=GAGGATTTGAAGGTTTTGCTTG
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=60.723
PRIMER_RIGHT_3_TM=60.109
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=0.8321
PRIMER_LEFT_4_PENALTY=0.723171
PRIMER_RIGHT_4_PENALTY=0.108929
PRIMER_LEFT_4_SEQUENCE=TTCATCCCATTCCTTTGTCACT
PRIMER_RIGHT_4_SEQUENCE=CTTGAGGATTTGAAGGTTTTGC
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=214,22
PRIMER_LEFT_4_TM=60.723
PRIMER_RIGHT_4_TM=60.109
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_05133 Ps_contig_mira-and-tgicl-ass_franssen_36550
SEQUENCE=AGCACAAACATTTCCCCTTTTACTTTATTCCATGGCACACATATATATATAAACAAGAGATGAAGGCTTTTGGATGAACCTTATTTTACAGAACTATTTACATCTCAGCAACCTTAACCCCAAAATTCTCTAGAGCATACATCAAATCAAATAAAAATAAAGTTAACATCATAATGTTAAAAGAACTTGTCACTCTTGCTGAGAATGAAGGTGCCTTCTGAGTTGCTAATAGTCAACATTTTCTTATTGAGTTTCTTCTAGTTTGTTACAATTGGTGGATGTGGTTTTATTTAGTTTTCCTATTTGTGATCTTGAGGTATCAGTAACACCTATTGAAGCAGAAGAAATACCCTTTGGACCTGTCTTCTGTGTTGGCTTTCGGGGTTTCACCAGGGTCGTCTCGTCGAAACATTAATGCACTTGTACCAGGAGATTCTGGTGGGAGTACAATGTTATCGGATGAAGCCATCCGCATCGGAACAACTGGTTCTGATGGCGAAATGGTAGAGAGATACCGAAGGAGCATCTGTATGATTTGACTAAAATTAGGTCGATCGTTTGGATCCTCTTTCCAGCATGATGTTACAATCATAGCTAAATCCTCCGGAAGCTCTTCAGCACTAGGTCTTGTATTCTTAAAAGCAGCTGCATATGCTGCTTGTAGGTTAGACATGCCTTCAAAGGGCAACTTGTTATGGATGAGCTCCCACAACACAATTGCAAAGCTGTAGGCATCCACTTTATGATTGTAATGCTTCTTCTYNCCTTGTCTYANNNTRTNTTAGAGTACGGTGCTAGTTAAAGTTCGTGGAGCCA
TARGET=41,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05134 Ps_contig_mira-and-tgicl-ass_franssen_36552
SEQUENCE=CAATGCCTGATGAACGATCTCATTTGGTTTCATGGTTCCGCCGAGTACAAGTCAACAAGGAGAACATCCCTAAAGCAATCGATCAAACATTGAACCCCGACGAAGAAACCATGGAGAGCATATATAAAGTGGCTGAGCTAGCAGGCCATTGCACTGCTCGCGAACCATACCAGAGGCCAGATATGGGGCATGCAGTGAATGTCCTGGTTCCTCTAGTTGAGCAATGGAAGCCTACCAGTCATGAAGAAGAAGATGGTTATGGTATTGACCTTCACATGAGCCTCCCTCAAGCTCTGCAAAGATGGCAAGCCAATGAAGGCACTTCCACAGTGTTTAACGACACATCTTTCTCACAAACCCAATCTAGTATTCCTGCAAAACCTTCTGGATTCGCCGACTCCTTTGACTCAATGGACTGCCGATAACTAAAATGACAACCTCTAATTGCTTCTCAGTTGGGATTGAGAAAGTTAGGCTGTTCTGATCTAGCAGCATTGCGGAATTGTATCACAAATTTAATTTATTTATTTTTAAAATGTCAATTTTTTGGTGTTGTTACAGTCTCTCCTGATGTATAAGTCTGATATGGTTTATTGTATTTTTTAAAGCTAGCGTAGTTANGGTTTTTAGTTGGTTTTGGGGATTCTTAGATAGAAAATATTAAG
TARGET=519,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6503
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.649547
PRIMER_LEFT_SEQUENCE=GTATTCCTGCAAAACCTTCTGG
PRIMER_RIGHT_SEQUENCE=TCTAAGAATCCCCAAAACCAAC
PRIMER_LEFT=366,22
PRIMER_RIGHT=650,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.350
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.6751
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.649547
PRIMER_LEFT_1_SEQUENCE=ACTCAATGGACTGCCGATAACT
PRIMER_RIGHT_1_SEQUENCE=TCTAAGAATCCCCAAAACCAAC
PRIMER_LEFT_1=405,22
PRIMER_RIGHT_1=650,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=59.350
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=1.0410
PRIMER_LEFT_2_PENALTY=0.391473
PRIMER_RIGHT_2_PENALTY=0.649547
PRIMER_LEFT_2_SEQUENCE=TCAATGGACTGCCGATAACTAA
PRIMER_RIGHT_2_SEQUENCE=TCTAAGAATCCCCAAAACCAAC
PRIMER_LEFT_2=407,22
PRIMER_RIGHT_2=650,22
PRIMER_LEFT_2_TM=59.609
PRIMER_RIGHT_2_TM=59.350
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=1.2424
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=1.241665
PRIMER_LEFT_3_SEQUENCE=GTATTCCTGCAAAACCTTCTGG
PRIMER_RIGHT_3_SEQUENCE=TCCCCAAAACCAACTAAAAACC
PRIMER_LEFT_3=366,22
PRIMER_RIGHT_3=642,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=61.242
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=1.2530
PRIMER_LEFT_4_PENALTY=0.603423
PRIMER_RIGHT_4_PENALTY=0.649547
PRIMER_LEFT_4_SEQUENCE=TGGGATTGAGAAAGTTAGGCTG
PRIMER_RIGHT_4_SEQUENCE=TCTAAGAATCCCCAAAACCAAC
PRIMER_LEFT_4=456,22
PRIMER_RIGHT_4=650,22
PRIMER_LEFT_4_TM=60.603
PRIMER_RIGHT_4_TM=59.350
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_05135 Ps_contig_mira-and-tgicl-ass_franssen_36564
SEQUENCE=CCGGAAAGTCATTGATTTGTAACGAGATCAGTGCAACTCATTCTTAACATAATCAAGGGTATTGATTTCTTGGAGTTCAGGCCTTCTGAAATTGCTGCAGGGGTAGCAATTTCTGTTTTGAAGGAACTGCCGGGGCATGAAGTCGATAAGGCCATAGCCGATTTCGTCATTGTAGATAAGGAGAGAGTTCCAAAGTGTGTTGAATTGATAAAAGATCTATCCTTGATCAAGGTAGGTGCATCTAAGTATACACCATTTGTGCCTCAAAGTCCTATAGGGGTGCTTGATGGAGGGTGCATGAGTTTTAAGAGTGATGAGTTAACAAATGGGTCATATCAAAATTCTTTACAAAATAGTCCAAATACTAAAAGGAGAAAATTTGATGGACCTTCCAATGGATAATGATGAAATGTGAAGTTTGAAAGAGGTGTGTTTGGATGAATAAGTTGTTGTTTATAGTGAGATTATTATTACTATTATGATTACAGAAACAGCTGCAAAAAAATACTGCAGAGAGAGAGAGATGCTGCGGTTGGTAGAGAAAATTTTGATGAAACAATGTTGTTGAGGCATAGCATATGTGAATGAAGTGACATATGTAATATTTGAGAGGTTATAATTGTGTTGTTCACATGTGAATCTAAACCT
TARGET=512,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0119
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.003602
PRIMER_LEFT_SEQUENCE=TTGAAAGAGGTGTGTTTGGATG
PRIMER_RIGHT_SEQUENCE=CAAAATTTTCTCTACCAACCGC
PRIMER_LEFT=418,22
PRIMER_RIGHT=549,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=11.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.0183
PRIMER_LEFT_1_PENALTY=0.014720
PRIMER_RIGHT_1_PENALTY=0.003602
PRIMER_LEFT_1_SEQUENCE=CAAAGTCCTATAGGGGTGCTTG
PRIMER_RIGHT_1_SEQUENCE=CAAAATTTTCTCTACCAACCGC
PRIMER_LEFT_1=264,22
PRIMER_RIGHT_1=549,22
PRIMER_LEFT_1_TM=60.015
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=11.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.0942
PRIMER_LEFT_2_PENALTY=0.090580
PRIMER_RIGHT_2_PENALTY=0.003602
PRIMER_LEFT_2_SEQUENCE=ATGGACCTTCCAATGGATAATG
PRIMER_RIGHT_2_SEQUENCE=CAAAATTTTCTCTACCAACCGC
PRIMER_LEFT_2=382,22
PRIMER_RIGHT_2=549,22
PRIMER_LEFT_2_TM=59.909
PRIMER_RIGHT_2_TM=60.004
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=11.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.3415
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.333229
PRIMER_LEFT_3_SEQUENCE=TTGAAAGAGGTGTGTTTGGATG
PRIMER_RIGHT_3_SEQUENCE=AAATTTTCTCTACCAACCGCAG
PRIMER_LEFT_3=418,22
PRIMER_RIGHT_3=547,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.667
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=130
PRIMER_PAIR_PENALTY_4=0.3479
PRIMER_LEFT_4_PENALTY=0.014720
PRIMER_RIGHT_4_PENALTY=0.333229
PRIMER_LEFT_4_SEQUENCE=CAAAGTCCTATAGGGGTGCTTG
PRIMER_RIGHT_4_SEQUENCE=AAATTTTCTCTACCAACCGCAG
PRIMER_LEFT_4=264,22
PRIMER_RIGHT_4=547,22
PRIMER_LEFT_4_TM=60.015
PRIMER_RIGHT_4_TM=59.667
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_05136 Ps_contig_mira-and-tgicl-ass_franssen_36565
SEQUENCE=TAAAGGATTTGTTGGATTTTCTAGATGAGCTTATAAGTCCATTGAGGAGTAGAATGAATGAGCAACAAAACATTCATGTAGAAGACACAAAAACAAGAGAAGAAACAATATCAGCTTCTGAATCTCCAAATTCCTCCTCATCTTCATACAAAGAATTATTGTCCAAAACCAACAAAGACACCCCACTTGGAAGTAGTAGTAGTAACGATGAGACGATGGTGAGAAAGAAGAGGATGATAAGAAGCACAAGAGTGGTGTATCCATTTGCATTAGTAAAACCTGGAGGAGAAGAAGATGAAGTGACATTGAATGACATAAACGAAAGGATGTTAATGGCACCAACAAGACCAGTAAGGCATCCAGTTGGTGACTTTGCATGTCGGCCATGCGTGTCTGACAACGAGGTCCCAGGTCCTTTACGAAGGGGAAAGCTAGGTGGGTTCGGCCCTTTAACTAGAACTCCACTA
TARGET=192,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2021
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.115769
PRIMER_LEFT_SEQUENCE=TGTCCAAAACCAACAAAGACAC
PRIMER_RIGHT_SEQUENCE=GTTGGTGCCATTAACATCCTTT
PRIMER_LEFT=159,22
PRIMER_RIGHT=342,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=60.116
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.2192
PRIMER_LEFT_1_PENALTY=0.086339
PRIMER_RIGHT_1_PENALTY=0.132857
PRIMER_LEFT_1_SEQUENCE=TGTCCAAAACCAACAAAGACAC
PRIMER_RIGHT_1_SEQUENCE=TCTTGTTGGTGCCATTAACATC
PRIMER_LEFT_1=159,22
PRIMER_RIGHT_1=346,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=188
PRIMER_PAIR_PENALTY_2=0.3303
PRIMER_LEFT_2_PENALTY=0.086339
PRIMER_RIGHT_2_PENALTY=0.243965
PRIMER_LEFT_2_SEQUENCE=TGTCCAAAACCAACAAAGACAC
PRIMER_RIGHT_2_SEQUENCE=GTGCCATTAACATCCTTTCGTT
PRIMER_LEFT_2=159,22
PRIMER_RIGHT_2=338,22
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=60.244
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.5099
PRIMER_LEFT_3_PENALTY=0.086339
PRIMER_RIGHT_3_PENALTY=0.423592
PRIMER_LEFT_3_SEQUENCE=TGTCCAAAACCAACAAAGACAC
PRIMER_RIGHT_3_SEQUENCE=GTCACCAACTGGATGCCTTACT
PRIMER_LEFT_3=159,22
PRIMER_RIGHT_3=370,22
PRIMER_LEFT_3_TM=59.914
PRIMER_RIGHT_3_TM=60.424
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.5099
PRIMER_LEFT_4_PENALTY=0.086339
PRIMER_RIGHT_4_PENALTY=0.423592
PRIMER_LEFT_4_SEQUENCE=TGTCCAAAACCAACAAAGACAC
PRIMER_RIGHT_4_SEQUENCE=AGTCACCAACTGGATGCCTTAC
PRIMER_LEFT_4=159,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=59.914
PRIMER_RIGHT_4_TM=60.424
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_05137 Ps_contig_mira-and-tgicl-ass_franssen_36568
SEQUENCE=AACGTTCCTCTTTGGTTCTGAGTCCAAAGAGTCTTGAATTGTGCACAGAGAATCTCGGCAATGAGAGTGGTACTGACATTGTGGAAAACGACATGTTATTGTCTTTAATGGGAAAAACGGAGGAAAGAGAACCTTGCCGTCAAGTTGTAGGTGGTTCTAAGAAAGCGAAGATTCAGAATTTTCCACCACCTTTGACGACGATAAGAGGATCGGAATCTCTTCGTGTTAGACCTCACCGTGAAGATGGAAGATTAGTGATTGAATTCACTAAAGTCCCACCGAGAACATCTTGTTTTGAAGCTGATAGAAGCCACGGCCGTCTTCGCCTTTGTTTTTCGACAAATCAAGAACAAGAAGAAGAAGATGACGATGATGGTGTGATAGATGAAAATGAAGAAGCACTCAATGAAGAAGAATTGTCTGAAAATGAAATGATTGGAGAAGAAATAAAAGATGTTGAAGAAGAAGAAGAAGAAGATACAGAGGAGGATGTGGTGGAAAGTGGAATTGTTGTTG
TARGET=352,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1327
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=CTAAAGTCCCACCGAGAACATC
PRIMER_RIGHT_SEQUENCE=AACAACAATTCCACTTTCCACC
PRIMER_LEFT=267,22
PRIMER_RIGHT=514,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.1327
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=ATTCACTAAAGTCCCACCGAGA
PRIMER_RIGHT_1_SEQUENCE=AACAACAATTCCACTTTCCACC
PRIMER_LEFT_1=262,22
PRIMER_RIGHT_1=514,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.1327
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=TAAAGTCCCACCGAGAACATCT
PRIMER_RIGHT_2_SEQUENCE=AACAACAATTCCACTTTCCACC
PRIMER_LEFT_2=268,22
PRIMER_RIGHT_2=514,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.2769
PRIMER_LEFT_3_PENALTY=0.146525
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=CACCGAGAACATCTTGTTTTGA
PRIMER_RIGHT_3_SEQUENCE=AACAACAATTCCACTTTCCACC
PRIMER_LEFT_3=276,22
PRIMER_RIGHT_3=514,22
PRIMER_LEFT_3_TM=60.147
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=0.4828
PRIMER_LEFT_4_PENALTY=0.352395
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=GAATTCACTAAAGTCCCACCGA
PRIMER_RIGHT_4_SEQUENCE=AACAACAATTCCACTTTCCACC
PRIMER_LEFT_4=260,22
PRIMER_RIGHT_4=514,22
PRIMER_LEFT_4_TM=60.352
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_05138 Ps_contig_mira-and-tgicl-ass_franssen_36568
SEQUENCE=AACGTTCCTCTTTGGTTCTGAGTCCAAAGAGTCTTGAATTGTGCACAGAGAATCTCGGCAATGAGAGTGGTACTGACATTGTGGAAAACGACATGTTATTGTCTTTAATGGGAAAAACGGAGGAAAGAGAACCTTGCCGTCAAGTTGTAGGTGGTTCTAAGAAAGCGAAGATTCAGAATTTTCCACCACCTTTGACGACGATAAGAGGATCGGAATCTCTTCGTGTTAGACCTCACCGTGAAGATGGAAGATTAGTGATTGAATTCACTAAAGTCCCACCGAGAACATCTTGTTTTGAAGCTGATAGAAGCCACGGCCGTCTTCGCCTTTGTTTTTCGACAAATCAAGAACAAGAAGAAGAAGATGACGATGATGGTGTGATAGATGAAAATGAAGAAGCACTCAATGAAGAAGAATTGTCTGAAAATGAAATGATTGGAGAAGAAATAAAAGATGTTGAAGAAGAAGAAGAAGAAGATACAGAGGAGGATGTGGTGGAAAGTGGAATTGTTGTTG
TARGET=459,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1327
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=CTAAAGTCCCACCGAGAACATC
PRIMER_RIGHT_SEQUENCE=AACAACAATTCCACTTTCCACC
PRIMER_LEFT=267,22
PRIMER_RIGHT=514,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.1327
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=ATTCACTAAAGTCCCACCGAGA
PRIMER_RIGHT_1_SEQUENCE=AACAACAATTCCACTTTCCACC
PRIMER_LEFT_1=262,22
PRIMER_RIGHT_1=514,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=253
PRIMER_PAIR_PENALTY_2=0.1327
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=TAAAGTCCCACCGAGAACATCT
PRIMER_RIGHT_2_SEQUENCE=AACAACAATTCCACTTTCCACC
PRIMER_LEFT_2=268,22
PRIMER_RIGHT_2=514,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.1337
PRIMER_LEFT_3_PENALTY=0.003322
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=AGAAGATGACGATGATGGTGTG
PRIMER_RIGHT_3_SEQUENCE=AACAACAATTCCACTTTCCACC
PRIMER_LEFT_3=358,22
PRIMER_RIGHT_3=514,22
PRIMER_LEFT_3_TM=59.997
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.2769
PRIMER_LEFT_4_PENALTY=0.146525
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=CACCGAGAACATCTTGTTTTGA
PRIMER_RIGHT_4_SEQUENCE=AACAACAATTCCACTTTCCACC
PRIMER_LEFT_4=276,22
PRIMER_RIGHT_4=514,22
PRIMER_LEFT_4_TM=60.147
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_05139 Ps_contig_mira-and-tgicl-ass_franssen_36569
SEQUENCE=ATCAATCTGGACCCACTTGATAAAGAGTTTTAATATCGGTTGTAAGCTTTTGCAAAATCTCCACGCCTATGAAATTCCCCAAAATTATCATGAGGTCTTGTAGGATAAGAAGGCTCTGTACTGTTTGAGAACCTGTTCCTTGATTCCAAAGGTAAATTGGTTCCACCATAAGGAGGATTGACAGGGGACCTATCATAGTCAGCAAAATTCTGCTGTTTATAACCACGGTACCCAGGAGACATTTGCTGGATTGCCTGTTGTTGACGCCTCTGAGCGTCAAGCTTAAAAAACTCATCCCATTGTTTTGCGTGTGTGGCACTCACCAAAGCAACTTTATTCATGTACTTCTCCCTCAAATCTTTAATAGCCTCACGGTGTTTGAAGTTTTCTTCATCTTCTTCTCTGTCACGGATTTTCATTAGCTCCATTGCCTCCTTTTTATACTCCAACTCTAATTCTTCAAGAGTCTTAGGTGAAGCATTGTGATCAAACTGTTGGGCAGGTTGGATACCAACCTCTCTCCTATGAGAATCCCAACCATCATTTCTAAACGTGCTCATGTTATGATTACGCCCATCAAATGAATTATAAGAGTTTCCTGCATGTGAGGCTGCTTGTCTTGGAGATCTTGACTGGCTTGAACGACCTCTAGGTCCACTATTCCCATAAGATAGCTTTAGTTCAAAAAGTATCTTTATAATCTCATACTGAAACCATTGGAGCCAATCCTTTCAGTAAATACTCTTCGGGACGGAGATAATGCTTGAAGTCTAAGAACTTACCTCTTGCTTGTGGAGAATCTCTTGAAAACTTTTGACTGTCCTCTCGAACACGGTCATCGACAGCCCTCTCTTGCCTGCCAAACCTTTGCTGAAACTGGGGTGCATTCCGTGGTGTAGAATGAGATCTCTGAGGCTCAGAACGCTGAAACCGGGCATTGACAGGACTTCTCTGATGTGGTGGTGACCTTGATCGTTGTGGTGGCGGTGACCTTGATCGGTTCATGTGAGAACTGGAAGATCCTTTTTGTTCACCTTCCTCTCTTATCTTATGCACAGCATCAACACCTTTAGCCATAACTAATCTATCCTTACTGCTAACAATAATGAATTTCTCGTCCATCTTAATTTTGCAGCCAATATCCCTTTCAATCTTAGTTATAACTTTCTCGGTGAAAAGTGCTCTTACATTTTTGTCTCTTGCAGGTATCCTTTGAAATAATTCTTGAGACTTATGGTTTGCTCCAAGGGTAGATGGACAACCCTGAGTGAAATGACCTGATTCGCCACACAGTTTGCAAATCATTTCTTCTTCTTTTCTTTTCTTCCAATTTCTCTCAGTAGCTTTGGATTTAGCTTCCCAGACCTTAGCATCGCGGCTGATGAAACCAGTTGGAATAGCATTAGGGTCACGAACTTCATCTTCTTCGTCAGAACCAGGATTAGACCTTTTGTTTGATTTTGCTTTCTCTTGAGCAGATCCAGATCCTAGGGGACCAGTGTACTCTTTGTAAAGATCACGAAAATCGTCATCGATATCTGGATCGGGGATATTGGCCATTGATGAATACTCTCTGAGAGAGAAGTGGAACAGAAGAACGGAAACGAAGATAGATCCCGAG
TARGET=1312,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1943
PRIMER_LEFT_PENALTY=0.071684
PRIMER_RIGHT_PENALTY=0.122569
PRIMER_LEFT_SEQUENCE=ACTTTCTCGGTGAAAAGTGCTC
PRIMER_RIGHT_SEQUENCE=GATGCTAAGGTCTGGGAAGCTA
PRIMER_LEFT=1162,22
PRIMER_RIGHT=1373,22
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=59.877
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.2190
PRIMER_LEFT_1_PENALTY=0.071684
PRIMER_RIGHT_1_PENALTY=0.147295
PRIMER_LEFT_1_SEQUENCE=ACTTTCTCGGTGAAAAGTGCTC
PRIMER_RIGHT_1_SEQUENCE=TGGTTCTGACGAAGAAGATGAA
PRIMER_LEFT_1=1162,22
PRIMER_RIGHT_1=1437,22
PRIMER_LEFT_1_TM=59.928
PRIMER_RIGHT_1_TM=59.853
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.2220
PRIMER_LEFT_2_PENALTY=0.099438
PRIMER_RIGHT_2_PENALTY=0.122569
PRIMER_LEFT_2_SEQUENCE=ACAACCCTGAGTGAAATGACCT
PRIMER_RIGHT_2_SEQUENCE=GATGCTAAGGTCTGGGAAGCTA
PRIMER_LEFT_2=1257,22
PRIMER_RIGHT_2=1373,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=59.877
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=0.2467
PRIMER_LEFT_3_PENALTY=0.099438
PRIMER_RIGHT_3_PENALTY=0.147295
PRIMER_LEFT_3_SEQUENCE=ACAACCCTGAGTGAAATGACCT
PRIMER_RIGHT_3_SEQUENCE=TGGTTCTGACGAAGAAGATGAA
PRIMER_LEFT_3=1257,22
PRIMER_RIGHT_3=1437,22
PRIMER_LEFT_3_TM=59.901
PRIMER_RIGHT_3_TM=59.853
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.2601
PRIMER_LEFT_4_PENALTY=0.137575
PRIMER_RIGHT_4_PENALTY=0.122569
PRIMER_LEFT_4_SEQUENCE=ACCCTGAGTGAAATGACCTGAT
PRIMER_RIGHT_4_SEQUENCE=GATGCTAAGGTCTGGGAAGCTA
PRIMER_LEFT_4=1260,22
PRIMER_RIGHT_4=1373,22
PRIMER_LEFT_4_TM=59.862
PRIMER_RIGHT_4_TM=59.877
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=114
=
PRIMER_SEQUENCE_ID=SSR_05140 Ps_contig_mira-and-tgicl-ass_franssen_36575
SEQUENCE=AAAAACGGTTAAAACCGTTAAACTTAGGGTAAAACCGAAAAAACCCCTTTTTAACGGTTAACGGTTACCCGTAACCCCTTTACCTAACGGTTTTTTTAGGGTTTTCCCGTTCCTAAAACCAATACCTAGAAACTACAAAATTAGCAATAACATGACACAAACATCTTTCTCTAAGCCCCAACGATCATCCTCCAATCTAAGCAACCGTAAACCCTCTTTGATGAACATCAAAATTCCTTCTATAACCAAACCTCTCCCACAACAAAACACTGAAGAGGATAATAAGCACTATAGAGGTGTTAGAAGAAGACCATGGGGGAAATTTGCTGCAGAAATCCGTGACCCTAACAGAAAAGGGTCAAGGGTGTGGCTTGGAACCTTTGACACCGCCATAGAAGCTGCAAAAGCTTATGATAAAGCTGCTTTTCAGATGAGAGGAAGTAAGGCCATATTGAACTTTCCTCTAGATGTTGCAACCGATGAATCTACAGATTCATCGGTTTCACTTTCTAGCTCAAAATACATCAAAGTTGGCGAGAAAAGACAAAGAGAAGAAGGTAATTACGTTGAAATTGAGATAAAAAGCAGTAGTAGTAATAATAATAAACAAGTGAAGAAAGAAGAAGGAGTTTCAGTTTGTCCTCTAACTCCTTCATGTTGGAAAGGGTTTTGGGATACTGATGTTATGGGAACTATCTTCAGTGTACCTCCTTTGTCACCCTTATCTCCACTTATGGGATGTTGAGATTAATTCATAATGGCTCAAAGTAAATGGACATATATGGAGAGTGTTTCGATGATGCAAGGGGTGCTAATAGTGTAAATACTATATAGGAAGTGTGAAGATAGTGCTTAATTCTTTAATTACGTTGTATATGTTCCTTTGGTCCAAGAATATTGAAATTTGTTCATTCGTTTATTAGTA
TARGET=595,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1534
PRIMER_LEFT_PENALTY=0.072552
PRIMER_RIGHT_PENALTY=0.080887
PRIMER_LEFT_SEQUENCE=AAGTTGGCGAGAAAAGACAAAG
PRIMER_RIGHT_SEQUENCE=ACACTATTAGCACCCCTTGCAT
PRIMER_LEFT=527,22
PRIMER_RIGHT=820,22
PRIMER_LEFT_TM=59.927
PRIMER_RIGHT_TM=59.919
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=0.1695
PRIMER_LEFT_1_PENALTY=0.033470
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=GATGTTGCAACCGATGAATCTA
PRIMER_RIGHT_1_SEQUENCE=GTGGAGATAAGGGTGACAAAGG
PRIMER_LEFT_1=466,22
PRIMER_RIGHT_1=730,22
PRIMER_LEFT_1_TM=59.967
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.1907
PRIMER_LEFT_2_PENALTY=0.072552
PRIMER_RIGHT_2_PENALTY=0.118120
PRIMER_LEFT_2_SEQUENCE=AAGTTGGCGAGAAAAGACAAAG
PRIMER_RIGHT_2_SEQUENCE=CTTGCATCATCGAAACACTCTC
PRIMER_LEFT_2=527,22
PRIMER_RIGHT_2=805,22
PRIMER_LEFT_2_TM=59.927
PRIMER_RIGHT_2_TM=59.882
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.2086
PRIMER_LEFT_3_PENALTY=0.072552
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=AAGTTGGCGAGAAAAGACAAAG
PRIMER_RIGHT_3_SEQUENCE=GTGGAGATAAGGGTGACAAAGG
PRIMER_LEFT_3=527,22
PRIMER_RIGHT_3=730,22
PRIMER_LEFT_3_TM=59.927
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.3129
PRIMER_LEFT_4_PENALTY=0.232060
PRIMER_RIGHT_4_PENALTY=0.080887
PRIMER_LEFT_4_SEQUENCE=ATCAAAGTTGGCGAGAAAAGAC
PRIMER_RIGHT_4_SEQUENCE=ACACTATTAGCACCCCTTGCAT
PRIMER_LEFT_4=523,22
PRIMER_RIGHT_4=820,22
PRIMER_LEFT_4_TM=59.768
PRIMER_RIGHT_4_TM=59.919
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_05141 Ps_contig_mira-and-tgicl-ass_franssen_36581
SEQUENCE=AGTCAACAAGTCCTTTCTTGGGTATCTCATAATTGACAAGCCATTTATAAAAATGTGAAGTATAAAAATTGGGTATAGTACTGAAACAACATGCAGAAAAGATACATTAAAGATTGTACATTAGCCACAAACTAAACTCACAGTGACCTTACACTACTAGATACATACAAAGCATACAAAAAGTAAATGTGATTACTGATACAACATACTGTGATTTCACAGAATAAAATCTGCAGCACTCTCACCAATAAACGCGATTCAGAGATGCGAAACACTTATTAGTTCACCACCATTGGAAAGAATGCTACTGCGATTCAGAGACTTCAGAGACGAACACGGCTGACGTCTAATTGAGAGCATTAGATGCGGAGTATATGAAATCCAAGAAACCTGTTCCTTTTAAGAGTCCCTTACCTGTTAAAAGTTCAAAATCCAACAACAGAAGGAATCCAATAACGGCTGCTTTGCCGTTTATTATTTCGTTCTTTCTACTGAAGCCGAATCCCAGTTGACTCTCATCAACCACCATTTTAACCTCATCGACGTTGACATCCGATGCTGTTATACCGGGTTGCGCCTTCCCACTAAAGACAATTTCAGTAGCAGCATCAGATTTGAAACCTCCTGTGATGCTGATATAAATGTTCTTTTCATCGGTTTTGACGGGATATACAAAGAGATTCCTCAATGCCGGTGTTAGAACTCTCAAAACAGGATTGTTTGGATACCATTCTTTGATCTCTCCTGTTCTTAGATCAAATGTGCTATCCGTTGTTGGACAAACTACACAACCATCCTGAGTGAGTTTAGCATTTTTCAATCCTTCTGAGTATGCACCTTCAGCAGGTGACCTATTTTCAATGGCGAATATTTGATCTTTATACCAAAGAAGCAATATAGTTTCCCCTTCTTGAATAATCACGCGCCGCTCTCCTCTGGGCAGCGCTGAGACTGGCACTACCGGAACCCAGTTTCCGGATGGAGATGACTCTTCAGTGACGGACACTTGTGTGGCTTTACAGGTGGTGGTGGTGGTGGTGGTGAGTCGAAGAGACTTTGGGCGTGCATGGTGGTTGGAGTGACGGGTGGAAAGGAGAAGAAACGGCTTACGAGGGAGAAGGAAATGGTGAGGAGTGTGTGTTTGGGAGGTGCAGGAAAGTGCTAAAGGGTTGGTGGCAGCCATGGAAAGGGGGTTGTGAGAAGAAGGAAAGTGGTGTTGGAGAAAGAAAGGATTTGCATGTGTGGAGGGATGGGTGGTGGGAGTAAGTCCACACAGTTACACGCAACTGGATAATGAATCATC
TARGET=1022,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3529
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=0.099438
PRIMER_LEFT_SEQUENCE=TCAGCAGGTGACCTATTTTCAA
PRIMER_RIGHT_SEQUENCE=ACACACTCCTCACCATTTCCTT
PRIMER_LEFT=839,22
PRIMER_RIGHT=1138,22
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=59.901
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.3636
PRIMER_LEFT_1_PENALTY=0.253482
PRIMER_RIGHT_1_PENALTY=0.110131
PRIMER_LEFT_1_SEQUENCE=TTCAGCAGGTGACCTATTTTCA
PRIMER_RIGHT_1_SEQUENCE=CACTCCTCACCATTTCCTTCTC
PRIMER_LEFT_1=838,22
PRIMER_RIGHT_1=1135,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.3636
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.110131
PRIMER_LEFT_2_SEQUENCE=TCAGCAGGTGACCTATTTTCAA
PRIMER_RIGHT_2_SEQUENCE=CACTCCTCACCATTTCCTTCTC
PRIMER_LEFT_2=839,22
PRIMER_RIGHT_2=1135,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=0.4573
PRIMER_LEFT_3_PENALTY=0.347129
PRIMER_RIGHT_3_PENALTY=0.110131
PRIMER_LEFT_3_SEQUENCE=CCTTCAGCAGGTGACCTATTTT
PRIMER_RIGHT_3_SEQUENCE=CACTCCTCACCATTTCCTTCTC
PRIMER_LEFT_3=836,22
PRIMER_RIGHT_3=1135,22
PRIMER_LEFT_3_TM=59.653
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.6612
PRIMER_LEFT_4_PENALTY=0.649076
PRIMER_RIGHT_4_PENALTY=0.012153
PRIMER_LEFT_4_SEQUENCE=CGGATGGAGATGACTCTTCAGT
PRIMER_RIGHT_4_SEQUENCE=TTTCTCCAACACCACTTTCCTT
PRIMER_LEFT_4=975,22
PRIMER_RIGHT_4=1224,22
PRIMER_LEFT_4_TM=60.649
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=250
=
PRIMER_SEQUENCE_ID=SSR_05142 Ps_contig_mira-and-tgicl-ass_franssen_36584
SEQUENCE=GATTACTAATTTATCATACGCGAATCCGAAAACATGTGTTCCTTTCACATGACCTGTTACTACTAAATTTCTTGTTCTTCAACCCAAAGTTTTGTTTCCTTTTTCTTCCACCACAACTAAAATTTTCGGANNNNNGAATCCTAAAAAGTAACTTATTTTTCTTCTATTCTCTCTCTCTCTCTGCTGTGTTGTGTACTTATAAACCGTTGAAGAGAAGCTCAGAAAAGTGACTCGTGTTTGTTGAGAATTGTGACACAGAAACTAATGGGTAACTGTCTTGATTCTTCTGCTAAAGTTGATGCAGCTCAAAGTTCTAGATCCACTTCTGGAATTTCGAAAACTACTCCTTCTAGTTTAACAATTCCATCATACAGTGACAAAAGCAATTCCTCTAGTCTTCTTCCAACACCGAGGTCTGAGGGGGAAATCTTGTCTTCTCCTAATCTTAAAGCTTTTTCATTCAATGAGCTAAAGAATGCTACGAGGAATTTTCGTCCCGATAGTCTTCTGGGTGAAGGAGGATTCGGTCATGTTTATAAGGGATGGATAGATGAACAGACTTATACGGCTGCGAAACCTGGATCGGGTATGGTTGTTGCTGTGAAAAGACTTAAGCCGGAAGGTTTTCAAGGTCATAAAGAATGGTTGACTGAGGTTAACTATTTAGGACAACTGCATCATCCTAATCTGGTTAAATTAATTGGATACTGCTTGGAGGGAGACAACAGGCTTTTGGTATATGAGTTTATGCCTAAAGGGAGCTTAGAAAACCATTTGTTTAGAAGAGGACCACAACCACTGTCTTGGCCGGTGAGAATGAAAGTGGCTATTGGTGCTGCAAGAGGACTCTCTTTTCTTCACAATGCCAAATCACAAGTTATATACCGCGATTTCAAAGCTTCTAACATCCTTTTGGACGCGGAGTTTAATTCGAAACTATCCGATTTTGGCTTGGCTAAAGCGGGTCCTACCGGTGATAGAACTCATGTGTCTACTCAAGTTGTGGGTACTCAAGGATATGCAGCACCTGAATATGTTGCAACTGGTCGGCTGACGGCTAAGAGTGATGTATACAGCTTTGGAGTTGTTTTGCTCGAACTTCTATCAGGAAGACGCGCGGTGGACAAAACAATAGCCGGCGTGGACCAGAATCTAGTAGACTGGGCAAAACCATATTTAGGCGATAAGCGAAGACTATTCCGAATCATGGATTCAAAATTAGAAGGCCAATATCCGCAAAAAGGAGCATTCATGGCTGCTACACTTGCTCTACAATGCCTTAACAGAGAAGCTAAAGCAAGGCCTCCAATGATAGATGTTTTAGCAACTCTTGAACAGATAGAAGCTCCTAAACAAGCAGCCCGGATTTCACTATCCGAACAGCAAAGAGTTCATGCTCCGGTGAGAAGGTCTCCTGCGCGAAACCGAACACCTTTAAATGTAACTCCTACTGCATCGCCACTGCCATCTGTTCGGCAATCTCCTCGCGCGCATTAGTTGCCGCATGAGGAATGTTCTGTATGTAAATTTGTTATTTTTAGAGGTTGTGTTTTTGACAAGCATGTTTTGTGAGCTGTGGCATATCTTTTCAATTTGCGCCTACATCATAAACACATCATTATATACCAANCCAAACCG
TARGET=168,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0662
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.054021
PRIMER_LEFT_SEQUENCE=TCCTTTTTCTTCCACCACAACT
PRIMER_RIGHT_SEQUENCE=CTGAGCTTCTCTTCAACGGTTT
PRIMER_LEFT=96,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.054
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=126
PRIMER_PAIR_PENALTY_1=0.0866
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.074466
PRIMER_LEFT_1_SEQUENCE=TCCTTTTTCTTCCACCACAACT
PRIMER_RIGHT_1_SEQUENCE=TCGAAATTCCAGAAGTGGATCT
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=336,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.3308
PRIMER_LEFT_2_PENALTY=0.276812
PRIMER_RIGHT_2_PENALTY=0.054021
PRIMER_LEFT_2_SEQUENCE=CATGTGTTCCTTTCACATGACC
PRIMER_RIGHT_2_SEQUENCE=CTGAGCTTCTCTTCAACGGTTT
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=221,22
PRIMER_LEFT_2_TM=60.277
PRIMER_RIGHT_2_TM=60.054
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.3380
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=0.325885
PRIMER_LEFT_3_SEQUENCE=TCCTTTTTCTTCCACCACAACT
PRIMER_RIGHT_3_SEQUENCE=TTTGAGCTGCATCAACTTTAGC
PRIMER_LEFT_3=96,22
PRIMER_RIGHT_3=309,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=59.674
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.3844
PRIMER_LEFT_4_PENALTY=0.364634
PRIMER_RIGHT_4_PENALTY=0.019805
PRIMER_LEFT_4_SEQUENCE=TTCCTTTTTCTTCCACCACAAC
PRIMER_RIGHT_4_SEQUENCE=GTTTTCGAAATTCCAGAAGTGG
PRIMER_LEFT_4=95,22
PRIMER_RIGHT_4=340,22
PRIMER_LEFT_4_TM=60.365
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_05143 Ps_contig_mira-and-tgicl-ass_franssen_36598
SEQUENCE=TCGGGATTAAAAATATTAATAATTACTCCCTTTTCCCTTCTTTCTTTATTCTTTTCTTTTCTTTTCTTTTCTTTCTTCCCTCTTTCCTTCTTCCTACTTTTTAATGTTCTTTTAAGGAAGAAAAAAGAAATCACGAGTGTGAGTGAGTGCTACCTTTCAAACTCGAAAAGATGGAGAAAATCAACTTTGTCGAAAATGGTGAATTGAAAATGCCACCAGGTTTTCGTTTTCATCCAACCGATGAAGAACTTGTTCTTTCCTATTTGAAACGTAAGGTCTTCTCTCTCCCTTTGCCTGCTGCAATCATCCCTGAAGTTGATCTCTGCACRGTCCCGACCCAGTGGGTACTTTTACCCGGTGTATTTAGGAAAC
TARGET=49,25
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05144 Ps_contig_mira-and-tgicl-ass_franssen_36599
SEQUENCE=GGCTATGGTGGAGGTGGAGGCTATGGTGGCGGTGGTGGATATGGCGGCGGTGGTGGATATGGAGGCGGTGGCGGATATGGTGGTGGTGGCTTTGGCGGTGGTGGCTATGGTGGTAGTACTTATGGTCGTAATGATAATAACAACAGTGGAAGCTATGGAAGTGGTGGTTATGCTGCCCCAGGAAGCTATAGTGGTGGCAATGCCGAGACTAGTTACGCTGCTCCTGGTGAACACGGAAGTAACTCTAATTTGGGATATGATGGTCAACTTGGTAGCCACCAAGATGGCGGTGTATTCGTTGAGCCACTTGAAAACAACCATGTGACAGAGAACAATGACGAACCTAATGACTACGCAGAAACCCAGAGGTGAGGTGAGAGTATCTTCATCATGAAGGAATTCCTTGCAAAGAGTAAGTAACTTGTGGCATTGAGTGGTATAAACACATGTTTCAAATATTTGTTTACTTTTGTTTTTCAGAACTTTTCATTGTTTGGCATAAATTTGATAGTTTGTCAGCTCAAGGACTGATGTAAAAATAAGACCTTTATAGGATTGATTGCAACTACATTTATAAATTTTGTTTCCCTTTTGTTGNTTGGCATGTTTTGAGAATGGTGCAACAATCAAAATTGATGGAAATTTTAACTTGAAGTTGAATACGAGGGGGTTAACCAAAGTTCAAGAGGATTTTATGTCCCTTTAATTTTGAAACC
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6326
PRIMER_LEFT_PENALTY=1.579280
PRIMER_RIGHT_PENALTY=0.053348
PRIMER_LEFT_SEQUENCE=CTATGGTGGAGGTGGAGGCTAT
PRIMER_RIGHT_SEQUENCE=ACCAAGTTGACCATCATATCCC
PRIMER_LEFT=2,22
PRIMER_RIGHT=272,22
PRIMER_LEFT_TM=61.579
PRIMER_RIGHT_TM=59.947
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=1.7713
PRIMER_LEFT_1_PENALTY=1.717979
PRIMER_RIGHT_1_PENALTY=0.053348
PRIMER_LEFT_1_SEQUENCE=TATGGTGGAGGTGGAGGCTAT
PRIMER_RIGHT_1_SEQUENCE=ACCAAGTTGACCATCATATCCC
PRIMER_LEFT_1=3,21
PRIMER_RIGHT_1=272,22
PRIMER_LEFT_1_TM=60.718
PRIMER_RIGHT_1_TM=59.947
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=1.8943
PRIMER_LEFT_2_PENALTY=1.579280
PRIMER_RIGHT_2_PENALTY=0.314970
PRIMER_LEFT_2_SEQUENCE=CTATGGTGGAGGTGGAGGCTAT
PRIMER_RIGHT_2_SEQUENCE=CAAGTTGACCATCATATCCCAA
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=270,22
PRIMER_LEFT_2_TM=61.579
PRIMER_RIGHT_2_TM=59.685
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=2.0270
PRIMER_LEFT_3_PENALTY=1.579280
PRIMER_RIGHT_3_PENALTY=0.447703
PRIMER_LEFT_3_SEQUENCE=CTATGGTGGAGGTGGAGGCTAT
PRIMER_RIGHT_3_SEQUENCE=AGTTACTTCCGTGTTCACCAGG
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=61.579
PRIMER_RIGHT_3_TM=60.448
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=2.0329
PRIMER_LEFT_4_PENALTY=1.717979
PRIMER_RIGHT_4_PENALTY=0.314970
PRIMER_LEFT_4_SEQUENCE=TATGGTGGAGGTGGAGGCTAT
PRIMER_RIGHT_4_SEQUENCE=CAAGTTGACCATCATATCCCAA
PRIMER_LEFT_4=3,21
PRIMER_RIGHT_4=270,22
PRIMER_LEFT_4_TM=60.718
PRIMER_RIGHT_4_TM=59.685
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_05145 Ps_contig_mira-and-tgicl-ass_franssen_36604
SEQUENCE=GTCGGGGGAATTCTCTCTCTCTCTCTCTTATTTATCTTTCTCTTTTCTATTTCTCTTTAAAAAGCAAGCACACACCATGGCACATATCCTAAGTGAAGAACAAATTGTTGATTTCAAAGAAGCCTTTGGTTTGTTTGACAAAGATGGAGATGGTTGCATTACTGTGGAAGAATTGGCCACTGTTATTAGGTCACTGGATCAGAATCCCACTGAGGAAGAGCTACAAGATATGATAAGTGAAGTTGATGCAGATGGCAATGGAACCATTGAATTTGATGAGTTCTTGAACTTGATGGCAAGGAAAATAGAAAGACACTGATGCAGAAGAGGAGCTAAGAGAGGCTTTCAAGGTTTTTGTATAAAGATCAAAATGGCTATATATCTGCCAGTGAGTTGAGACATGTAATGATGAATCTAGGTGAAAAATTAAGTGATGAGGAGGTGAAGCAGATGATTAAAGAAGCAGATATGGATGGTGATGGTCAAGTTGATTATGATGAATTTGTGAAGATGATGATGGCCATTAGATGAAACTATTTCACCTATAAATTCACTGCCTTTATTTAATTCCAACAACCTCATTTTATTTTATTTCAAATTGTTGTTTGTTTTATTTTTTACTTGTACCAAGTGGCCCCATAAATTGGTGCTAACAAAGTATTAGTTAGTTAACCACATCATTCTAGTTCCAACTCAAAAATGTTATTATTTTAATCCTTGTTTAGTGTTATATAATATAACAGTCAAGTTCTTCTGTTAGCGGCATTTTAATTCT
TARGET=12,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05146 Ps_contig_mira-and-tgicl-ass_franssen_36622
SEQUENCE=AATCAACCNAAACAAAATAAAACAAATCGCCAAATCAAATCTCATCGTCACCGATTTTCCAAACTCGCAAGAACGAACAATCACCCGATTCATCATCCTCATCGTTCCCATGGTATAACAATATCACAGTAGTTTGAACAAGGATTTTGAAACTTAAGAAATTTTGTTGTTAATGGCTTAAACCTAAAAAAGAGGGTTAAGGTTGTGTGTACTATGGAGCTTGATTTGAATCAGGAGCCTTTGGATCGAAGTAGTAGTTCAGTAGTTGAATTTGATTCTGTATTGGACGAACTCGAAGCTGCCGAGGGAAATATTCGGAACCGAATAAGACACCTTGAAGAGATTACTTCGAGAGCGAGGCAGAGTCGGAGGTTGCCGCGGATGATGATGAATTCGCCGATTCGGATAACTAATTTTACGGGAGAAGCGATGACTGATGATGATGTTCGAGGTGAAGAAGAAAGGGAGGAGCAGAATGATGTTGAAAAATGTGGAAAAAGAAAGAGTGCTCATTTAGTAGCGAAAGCGTTGGGGGTGGAAGAGATTGATGATGGTAAAGTGGAGGAGAGTAGTGAGAGTTTTTATGATTGTAATATATGCTTGGATGTTGCGAGAGATCCTGTTTTGACGTGTTGTGGGCATTTGTTTTGTTGGCCGTGTTTCTATCAGTTATCGTATGTTTATTCGAAAGCGAAGGAATGTCCTGTTTGTAAAGGAGAGGTTACTGAATCGGGTATTATTCCGATTTATGGGAAAGGTAGTGTTGGTGGTGATTGTCAAATGGAAATGAAAGAAGCTGGTTTGAGAGTTCCTCCTCGACCTAAGGCTCCTAGAATTGAGAGTATTAGACAGAAGTTGATAACCCGAGGTGCATCTTCTTCTTCTATTGTCCAAAGCATTCGGCGGTTTCATAACCGAATTGGTGGATTTGGAGAACAGGCTCAGTTAGAAAGCCCTAATACCAGAGGTGATAGAAATAATGGTACGCTCGTTCGTTCTCGCACGCAGACTGATATTAATCAACACGCAGGCACTCATCAAGTTTCAAGGATGCTAGAACAAGGAGCTTCTTCATTTTCATCGCTTTCATCAGCGATAAATTCTGCAATGGATTCTGCAGAAAGATTAGTTCAGGACCTTGAATCTTATATCCACGGCCACAATATAGGAGGAAGCAGAGAGTTAAACCATCTTGCTGAAAATAGAAGTTCAATGTTTAGTTTCGCTGCTACAAACCGCTCGGAGAGTCGTGCTCAAGATGTTGTTGATGTGGCCAATTCTGCTGCTGCAGCAGCTAGGAATGTTGACAATATTGCTGCTAGTGGTTCAGAAATTCAGACAACTGATAAAGACATATATATTAGTCCGTTAGACCCTTCTTCGTCAAACAGTAGAAGAAGAAACGGTGCTTCAAGACAAGTTTCAAATGAACCCAGCAGAAGAAGAAGATTGAGATGATCATCTTTTATGTAAGGGGTAACGCATTATATTTATCATAGGTTCCTCGTTTTGTTGCATGCATACTACAATTTGTTTGTCATACACAATTTTCATTCACAGTCTAAAACAAATTTGTTATGTTATTTATGTATATATAGCGCATTTTATTTAACAGATGTAAGGATATTTGTCTGTTTGGTTTGGATGATTTGAGCCTATTTGTCTGTTATGGAAACAATTTATGTCATGTCCATAAGCTGTAGTTAAAANTNANTTT
TARGET=874,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0093
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.006951
PRIMER_LEFT_SEQUENCE=GACAGAAGTTGATAACCCGAGG
PRIMER_RIGHT_SEQUENCE=AGCGATGAAAATGAAGAAGCTC
PRIMER_LEFT=847,22
PRIMER_RIGHT=1084,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.0373
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=GACAGAAGTTGATAACCCGAGG
PRIMER_RIGHT_1_SEQUENCE=TAGGGCTTTCTAACTGAGCCTG
PRIMER_LEFT_1=847,22
PRIMER_RIGHT_1=957,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=111
PRIMER_PAIR_PENALTY_2=0.0778
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.075444
PRIMER_LEFT_2_SEQUENCE=GACAGAAGTTGATAACCCGAGG
PRIMER_RIGHT_2_SEQUENCE=ACGAACGAGCGTACCATTATTT
PRIMER_LEFT_2=847,22
PRIMER_RIGHT_2=995,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=59.925
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.0778
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.075444
PRIMER_LEFT_3_SEQUENCE=GACAGAAGTTGATAACCCGAGG
PRIMER_RIGHT_3_SEQUENCE=AACGAACGAGCGTACCATTATT
PRIMER_LEFT_3=847,22
PRIMER_RIGHT_3=996,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=59.925
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=150
PRIMER_PAIR_PENALTY_4=0.0886
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.086339
PRIMER_LEFT_4_SEQUENCE=GACAGAAGTTGATAACCCGAGG
PRIMER_RIGHT_4_SEQUENCE=CTTGAAACTTGATGAGTGCCTG
PRIMER_LEFT_4=847,22
PRIMER_RIGHT_4=1048,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=59.914
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_05147 Ps_contig_mira-and-tgicl-ass_franssen_36622
SEQUENCE=AATCAACCNAAACAAAATAAAACAAATCGCCAAATCAAATCTCATCGTCACCGATTTTCCAAACTCGCAAGAACGAACAATCACCCGATTCATCATCCTCATCGTTCCCATGGTATAACAATATCACAGTAGTTTGAACAAGGATTTTGAAACTTAAGAAATTTTGTTGTTAATGGCTTAAACCTAAAAAAGAGGGTTAAGGTTGTGTGTACTATGGAGCTTGATTTGAATCAGGAGCCTTTGGATCGAAGTAGTAGTTCAGTAGTTGAATTTGATTCTGTATTGGACGAACTCGAAGCTGCCGAGGGAAATATTCGGAACCGAATAAGACACCTTGAAGAGATTACTTCGAGAGCGAGGCAGAGTCGGAGGTTGCCGCGGATGATGATGAATTCGCCGATTCGGATAACTAATTTTACGGGAGAAGCGATGACTGATGATGATGTTCGAGGTGAAGAAGAAAGGGAGGAGCAGAATGATGTTGAAAAATGTGGAAAAAGAAAGAGTGCTCATTTAGTAGCGAAAGCGTTGGGGGTGGAAGAGATTGATGATGGTAAAGTGGAGGAGAGTAGTGAGAGTTTTTATGATTGTAATATATGCTTGGATGTTGCGAGAGATCCTGTTTTGACGTGTTGTGGGCATTTGTTTTGTTGGCCGTGTTTCTATCAGTTATCGTATGTTTATTCGAAAGCGAAGGAATGTCCTGTTTGTAAAGGAGAGGTTACTGAATCGGGTATTATTCCGATTTATGGGAAAGGTAGTGTTGGTGGTGATTGTCAAATGGAAATGAAAGAAGCTGGTTTGAGAGTTCCTCCTCGACCTAAGGCTCCTAGAATTGAGAGTATTAGACAGAAGTTGATAACCCGAGGTGCATCTTCTTCTTCTATTGTCCAAAGCATTCGGCGGTTTCATAACCGAATTGGTGGATTTGGAGAACAGGCTCAGTTAGAAAGCCCTAATACCAGAGGTGATAGAAATAATGGTACGCTCGTTCGTTCTCGCACGCAGACTGATATTAATCAACACGCAGGCACTCATCAAGTTTCAAGGATGCTAGAACAAGGAGCTTCTTCATTTTCATCGCTTTCATCAGCGATAAATTCTGCAATGGATTCTGCAGAAAGATTAGTTCAGGACCTTGAATCTTATATCCACGGCCACAATATAGGAGGAAGCAGAGAGTTAAACCATCTTGCTGAAAATAGAAGTTCAATGTTTAGTTTCGCTGCTACAAACCGCTCGGAGAGTCGTGCTCAAGATGTTGTTGATGTGGCCAATTCTGCTGCTGCAGCAGCTAGGAATGTTGACAATATTGCTGCTAGTGGTTCAGAAATTCAGACAACTGATAAAGACATATATATTAGTCCGTTAGACCCTTCTTCGTCAAACAGTAGAAGAAGAAACGGTGCTTCAAGACAAGTTTCAAATGAACCCAGCAGAAGAAGAAGATTGAGATGATCATCTTTTATGTAAGGGGTAACGCATTATATTTATCATAGGTTCCTCGTTTTGTTGCATGCATACTACAATTTGTTTGTCATACACAATTTTCATTCACAGTCTAAAACAAATTTGTTATGTTATTTATGTATATATAGCGCATTTTATTTAACAGATGTAAGGATATTTGTCTGTTTGGTTTGGATGATTTGAGCCTATTTGTCTGTTATGGAAACAATTTATGTCATGTCCATAAGCTGTAGTTAAAANTNANTTT
TARGET=1438,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4159
PRIMER_LEFT_PENALTY=0.222249
PRIMER_RIGHT_PENALTY=0.193666
PRIMER_LEFT_SEQUENCE=TAGTCCGTTAGACCCTTCTTCG
PRIMER_RIGHT_SEQUENCE=AGTATGCATGCAACAAAACGAG
PRIMER_LEFT=1361,22
PRIMER_RIGHT=1524,22
PRIMER_LEFT_TM=59.778
PRIMER_RIGHT_TM=60.194
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.4297
PRIMER_LEFT_1_PENALTY=0.236064
PRIMER_RIGHT_1_PENALTY=0.193666
PRIMER_LEFT_1_SEQUENCE=TGCTCAAGATGTTGTTGATGTG
PRIMER_RIGHT_1_SEQUENCE=AGTATGCATGCAACAAAACGAG
PRIMER_LEFT_1=1250,22
PRIMER_RIGHT_1=1524,22
PRIMER_LEFT_1_TM=59.764
PRIMER_RIGHT_1_TM=60.194
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.5863
PRIMER_LEFT_2_PENALTY=0.392624
PRIMER_RIGHT_2_PENALTY=0.193666
PRIMER_LEFT_2_SEQUENCE=ACAAGTTTCAAATGAACCCAGC
PRIMER_RIGHT_2_SEQUENCE=AGTATGCATGCAACAAAACGAG
PRIMER_LEFT_2=1415,22
PRIMER_RIGHT_2=1524,22
PRIMER_LEFT_2_TM=60.393
PRIMER_RIGHT_2_TM=60.194
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=110
PRIMER_PAIR_PENALTY_3=0.5982
PRIMER_LEFT_3_PENALTY=0.404566
PRIMER_RIGHT_3_PENALTY=0.193666
PRIMER_LEFT_3_SEQUENCE=AAGAAGAAACGGTGCTTCAAGA
PRIMER_RIGHT_3_SEQUENCE=AGTATGCATGCAACAAAACGAG
PRIMER_LEFT_3=1394,22
PRIMER_RIGHT_3=1524,22
PRIMER_LEFT_3_TM=60.405
PRIMER_RIGHT_3_TM=60.194
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=0.7213
PRIMER_LEFT_4_PENALTY=0.527639
PRIMER_RIGHT_4_PENALTY=0.193666
PRIMER_LEFT_4_SEQUENCE=AGTCCGTTAGACCCTTCTTCGT
PRIMER_RIGHT_4_SEQUENCE=AGTATGCATGCAACAAAACGAG
PRIMER_LEFT_4=1362,22
PRIMER_RIGHT_4=1524,22
PRIMER_LEFT_4_TM=60.528
PRIMER_RIGHT_4_TM=60.194
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_05148 Ps_contig_mira-and-tgicl-ass_franssen_36630
SEQUENCE=GAAATTTTTATTACAGTAAAAAACTTTACCTGGTTCTTGACCTAGATCACACACTGTTAAATACCACTATCCTTAGTCATTTGAGCCCAGAAGAGTCGCATTTACGTAACGAGACAGATTCTCTAGAAGACGTCTCCCAAGGTAGCCTCTTTAAATTGGAGCATATGAATATGATGACCAAGTTGAGGCCCTTTGTCCGTACATTTCTTAAAGAAGCAAGTGAAATGTTTGAGATGTACATCTACACCATGGGTGATAGGCCATATGCATTGGAGATGGCCAAGCTCCTTGATCCTCAAGGAAAATACTTCAATGCTAAAGTGATATCTCGAGATGATGGGACTCAAAAGACATCAAAAGGGTCTTGATATTGTTTTTGGGCAAGAAAGTGCTGTCTTAATTCTCGATGATACAGATGATACATGAACATGCATGGATGAATCATAAAAGCAATCTGATACTTATGGAAAGATACCATTTTTTCGCTTCAAGTTGTCGGCAATTTGGCTTCAATTGCAAATCTCTTGCTGAAACGAAGAGCGATGAGAATGAAATAGATGGAGCACTTGCAAAAATACTTGAAGTGCTCAAGCAAATCCATAGGACATTCTTTGATAAACTTGAAGGAGATCTTGTTGACCGAGATGTGAGGCAGGTTTTGTCATCACTTCGAGGTGAAATCTTAAGCGAATGTGTGATCATTTTCAGTCGTATTAATCATGATGCATTGCCAACTCTCCAGAGAGTCGCACAGAAAATGGGTGCTACATGCTTGACGGAACTTGACCCCACAGTGACACATGTGGTTGCTACTGACGCTGGAACTGAGAAGTCCAGATGGGCAGTTAGAGAAAAGAAGTTTTTGGTTCATCCTCGATGGATAGAGGCTGCAAATTACTTTTGGCAAAAACAACCCGAAGAGAACTTTGGTCTAAAAAAGACCACTAACAACTCTTAGTATAGCACTGATCTATCTATCTATTGGTGATGAATCAAAAACTTCTCTTTAACTTCTTATTTTGGTAGAAGCGCAAAATGAGAATTTTTTTCTTTGATGAATCTAAAACTTCTCTCTTTTGAACTTCTTATTTTGGTAGAAGCAAAATGAGAACTTTCCCTTGTTTCAACACAAGCTTAGGCTTTTATGATAATTTTGTAGTTTATGAAACCAAAGACTTGGAAGGTTACAATGCTATTGCAATTATAACATGTACAGATTTTATTGGTTCCATNRTNTTATTGTATGAACTGCATGTTGTAAACC
TARGET=969,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05149 Ps_contig_mira-and-tgicl-ass_franssen_36631
SEQUENCE=AATCAACTTAATGTTTTTTTTATTATTTAAATCCTTCAAAGTAAATAAATTTTTAAAGTGAAAATCAAATACAAAACGAAATATCTAAATTAATGCAGATACAAGTCGTCCACTTTTCCATCATACATGATTCCATAACAACATTTTTCTTCATAATGTTTCCTTCCTACATAAAGGGAAAGGAAAATTTTCAACACATTTTTACTGATCCAATGAGTCATTTATGGCTCCAGCATATACAGTGTATCTTCAAAGCAGCAGGATTCTCCAGGCTAGGCATTTACATCAGGAGTGCGCAAAAGGATGATGGAAGAGTAACTAAGCATGGGATACATATTGRCATCAAGAAAATGAGCATACTGTTGTATTGCAATATCTCTTCCAGGAAGATCAGGCGTGAGGAAATCATATGGTGCGGACTTGCTGGTGTCTATGAGAGGTTCCCATCTGTATCCTGGACGCTCTGGCAAGGTAACTGTGAAAGGTAGATGACTCGTATTGAAAGCAATGTAAATTTCTCCCTTTACCGAGTCCACCAGGGTAAAAGCCACAAAACGGCTAGTTTCAGACCAGTCTGGAGTTACAGGAAAAAGACCATGCCCCTGCAGCCTCCCTGATGTTGGGAAGTCATCTAAACCAAGCGATTCACATTCTTGGCGAAACTTGATCAAAAGGCTGCAAAATCTGAAGAAGTCTGATGAGGACTCTTCCTTTTTGTCCCATTGGAAATAATTAAGATAATTATCATGACAGTAGGTATTGTTGTTTCCCCCTTTTGTATGTCCATATTCATCACCCATATGTATCATCGGAACTCCCTGGGAAACCATGAGAGAAAGAAAGAAATTACGCATTTGTCGTTTCCTCAATTTCTTTACTGAGGCACTGACAAACTCCCCCTCCTCTCCACAGTTCCAGCTATTATTATGATTTTCTCCATCATTATTGTCTTCTCCATTGGGCAAGTTATGCTTGCTGTTATAAGTCACCAAATCAGCTAGAGTGAAGCCATCATGAGCACATACAAGGTTAATACTATTCCATGGTTTTCTTCCTCCCTGATACAAACTAGGACTACCACA
TARGET=834,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05150 Ps_contig_mira-and-tgicl-ass_franssen_36637
SEQUENCE=GCAAAAGAATAAAAAAGTACCACGTGTGAAAAACATTTGTGGCCCAAAATGATAATTGCAGTAAAAGAATTACTATGACAGAGAAATGATCTAGTTTCCTGCACAGTTGCATAAAGTATACTAGTATATCACAGACAAGAAAAGAACCAAATTAAAGGGATAAAAACAAGAAGAAAAGTTGCAAGTTACAACAACATTACAAGGCCTTGTAAAAACCCCCAAGAAAATTCCCAACAGCGGCGGACAGTGATGGCAACTATAACTTTAGTGTCATGCAAGAGAATTGTTGGCTACTCGGTTTTCAAGGTATCGCTGCTTTTCCCACTGCTTGTTCAAATTTGTTCTTCTGGTGGAAAATTACGACTGTAGAGTGGAAATTCAAAGAGAAAAGCTGATTTGAACCATGCAGGTAACTTTCCATAGATTTCTGGTGGCAATGGGATTAAAGGGCTTGTTCTTTGTTTGTATAACCTATATCCGTCCACGTTGCCAAACTTCTTATCTGCTGATTCCTCGAGAAGTGGAATGCCACTGATGAAAAGAAGCAACAGTGTGAGAAAGATTGGTCCGAAGATTACCAGCCATTCAGCTTTCTTCAGTATAGGTGTAGATGCCACAAAAATTCCCCACCAAAGCAATATCTCGCCAAAATAGTTAGGATGACGAGTGTATTTCCAGAGTCCAGCATTGCACCACTTCCCTCTATTTTCTGTAGACTTTTTGAAAGTCAATTTTTGTTGATCCGCCGTAGCTTCAACCATAAAACCCACAGCCCACATAAGCCACCCAATCAAATCCACAGCCTTTAGAAAAGGATTTCTATCGCTTGCATTCACCACCGTAACAGGTAAACTCACAGCCCAAACCCAAACAGCCTGAAATATCCAAAACACAGCCAATCTCCCCAAATTATTGCGCATTTCATCAAAACGGCGATCCTCTCCCCACTGTAAAATCCTGAGTAAGAGAAAAAAAGCAAGGCGAAGACCCCATAATACAACAAAGAGAGTGAGGATTATCTGTCGAAAATGCCATGAGCCTTTGAGTACTAAGGTCAATACAGCCACTATAACGAAATTGGTACTTCCTGCAAAATCAGTGACCTTGTCGAATTTAAAAAGTGCAGTGATGATGAAAAACAGAAGCTGGTAAACAATGGTGATTATGGCAGTGAGGGCTAAGAAATGAGAGTCAATAACAGTGCCCATGCTTTTGTTATATTATTATATTCTTTGCTTTGCACTTTCGAGATATTGGTTTTTATGAATTTTTGTATTATATAGAATTTGAAAATAGAAAAAAGAAAAAGAAGAAGAAGAGA
TARGET=1307,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05151 Ps_contig_mira-and-tgicl-ass_franssen_36643
SEQUENCE=TTTTCTTYTYAATTCTAGCCCACTCTGATTGTCCCTCGGTGCAAAGCTGCTCCACTCTCTTGTGAAGAGCATGAGATTTTCCTTGTTTTGGGATCTCGACCGGAGAAAACTATTGGCTCCATTCCTCCATCTAGCTCAGGCAATGCAACTTGAAGAGCAACCTGAATTGGATGCAAACCCAAAGTACTATTCAACCACTCTTCCGTTGTCTGGAAAACCAGCGGCAATGCAACGATATAAGGAACATCAAGCTTCATCAAAGCTTCAATAGCTCTTGGATGATCCTGCCTAGCAGGACCTCCAACAAGAGCAAAGCCAGTGAGAGATATCACAGAATTCACAAAAGGTTTCTTAGTAATAGGATCAATCAAGAACCTCTCAACCGGCCCCGAAAAGTCCAACCCCCCAGCGAAAATCGGAATAACCTTAGCACCTTTAGCCTCAAGTTCCATAATCACAGCAACATAATGACCATCATCACCAGTAACAATATGACTCCTTTGCAAAATCAAACCAACAATAGGTGCAGTTGAACTCTTTAGCTTCTCATTGGCATCCCTTCTAGTTCCATACCAATTCAAATACTCTTTCACATCATCATACATACAAGGTGCTAAAGGGTGCCAAATTCCGTTATCTAAGAACAAAACCGGCTCTGAATAATCCATCTTTGCACCTTTCAATGCAGGAACATAAGAACCAGAAATCATCTTGAAGAAATTCTGCAAATTATCAGGTGATCCACCAAGCCAAAACTGAAGACTCAGTATATAAAGCCTAGCATCTTGAGCTTTATCACTTGGTAAATACTTCAAAACCTTAGGCAAAGTCCTCACAAGCTTCAACATGCTATCAGCAAATCCTGCAGAAGAAGTATTCTTCTTCTTGAAAAGCTGGAAAAAGGGACTCTTTGATTGTCCAAGCTGTGACATGCTGAATGATCCCAATTTGTTCAACCTCATCACTTCAGGCATTGATGGAAACACCAAAACTGCATCAAGTCTGTCCCTTTCTTTCTCAACAGCAGCTTTCACTTTGAGTGCAAGCTCTTCAACGAAGATCAAAGAACCAATGAAGATATTAGCATCTTCTAAATCCTTGCAGAAACAATTGTATGTCGATACATCACGAAGCTCTTCGACTAAGTAACCGACAACTTCAAAAGAAGCATCTTTTTGGTTGGTGTTGAGTCCTCTAACAGCTGCAGAGACGGATGATTGGTACTGAGCTTCAAGAACAACATACACAATTTTCACTGTTGGTAAATTCTGCTTATTCTCCGGAACTATTCTACGAACTTCTTGGGTTGTTTGAGTGAACAAACCGTTACCAATTGCATTGCATTTCACTCTTAAGGAGGCTTTTGAGGCACCATAGTAATTGGTTTTCTTTGGAAGGAAAGAATGAAGAAAGAGATGTTTTTGAGAAAGTGAAGAAAGTTGAACAGCTTTAGAATTTGGTAGAGTAAATGGTGTTGATACCAAAGAGGCCATTGATCATCAACCCAAGTTTCAGACAAAGAAAAACAAACTAAAAATCTAATCTTTTTACAATAAAGAGCAAATTTTGGTTATGAAGAAAGATGGGGTATGAGAAATTTGTACACTCTTTCTAATTTGTAGAGCCAAAGTTGAGTTGTGTTGTGAAATTCTCAACTCAACTCAACTTAGTTGAAACTGTCTTTTTCTTCTCTGTGTTGATGAGTTTTTATTTGATTTGTGTGGAGGATATGATAGAGAAAAAAGAGTCTCTTCCAATGAAAACGGTTTCTATGGTTAATGGTGAAAGCATGAACCACGTGGCGAACATATAGTCATTGGAATGAGAACTATTTTGAGGTTAAAGAGAGTGTGT
TARGET=1651,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05152 Ps_contig_mira-and-tgicl-ass_franssen_36650
SEQUENCE=CCAATTGTTGATTGTGGTTTTGTAAATTTCGTTGATTTCGAATCCTTGAAGTTAGGGTTTGAGAATTTGGGGATTTTAGGGTTTTGGTTTAATATTTTTTCCTTCAATTCTTTTGTGCTTGTCAATCCCATGGCGGCAGGGCGACAAAGTGTTTTGGGGAAGAGGGAGTCTTACAAACATGATTCCGTCAAGGAATTTCAGTATTCTAAGTATGATGATTCTCTTGCAAAAGAAGATAGAGAAGAAGGTGAGATCCTTGTTGATGGGGATGAAGCTTTGCCACCGCCAGAAAAGAGAAGAAAATTCTCTCCCATTGTCTGGGATCTGGCTGAGAAGAAGGCCAAAATCTCTTCCAACGATAGGGTCACACATGTTGCTGAATCTCTATCTCGATCACCTGTTGATGAACAAAGTGCTGAAGAAAAAGGTGTTTTAAGTGAGTTTTCGCTGAATTCCTCTTGTGTTGTTGATGAAAGTGATATGCAGGGGTGGAACATCACAAAGTCTAAATGGGCTTCTGATGATCTTTCACCTATGGGTGATGATGATAAGTACAAGCAAGACAAAAGAAGTTTAAGTTCGGAAGATGGTGGTTGTTCAGGTAGCACAATCACTAGATCTGATGTTGATGATGAGGTTAGTGATTCTCCATCAGATTCGTTTGAAAATAGTGATTTGATGCATGTGCAGAGGAATTTTAACATGTGTCAGAGTTGCAGAACTGTGTCGGAATTTGAAATGATTAAGAAAATAAATGAAGGAACTTATGGTGTTGTCTATAAAGCTAAGGATAAGAGGACTGGAGAGACTGTAGCACTAAAGAAGGTGAAGATGGAGAAGGAAAGAGAGGGATTTCCAATGTCAGCGTTGAGAGAAATGAATATTCTCTTGTCTTTAGATCATCCCTCTATTGTTGATGTTAAAGAAGTAGTTGTGGATGATGATGATAAGGATGATGGTACTTATATGGTAATGGAGCATATCCCGAACCTTGTATAGATCGCATTGCTGATACTGTTGGAAA
TARGET=937,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2962
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.236361
PRIMER_LEFT_SEQUENCE=AAGAGAGGGATTTCCAATGTCA
PRIMER_RIGHT_SEQUENCE=CAGCAATGCGATCTATACAAGG
PRIMER_LEFT=842,22
PRIMER_RIGHT=1010,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=59.764
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=0.3545
PRIMER_LEFT_1_PENALTY=0.118120
PRIMER_RIGHT_1_PENALTY=0.236361
PRIMER_LEFT_1_SEQUENCE=ATGTCAGCGTTGAGAGAAATGA
PRIMER_RIGHT_1_SEQUENCE=CAGCAATGCGATCTATACAAGG
PRIMER_LEFT_1=858,22
PRIMER_RIGHT_1=1010,22
PRIMER_LEFT_1_TM=59.882
PRIMER_RIGHT_1_TM=59.764
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=153
PRIMER_PAIR_PENALTY_2=0.3829
PRIMER_LEFT_2_PENALTY=0.146525
PRIMER_RIGHT_2_PENALTY=0.236361
PRIMER_LEFT_2_SEQUENCE=CAGAACTGTGTCGGAATTTGAA
PRIMER_RIGHT_2_SEQUENCE=CAGCAATGCGATCTATACAAGG
PRIMER_LEFT_2=716,22
PRIMER_RIGHT_2=1010,22
PRIMER_LEFT_2_TM=60.147
PRIMER_RIGHT_2_TM=59.764
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.4115
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.351615
PRIMER_LEFT_3_SEQUENCE=AAGAGAGGGATTTCCAATGTCA
PRIMER_RIGHT_3_SEQUENCE=ATGCGATCTATACAAGGTTCGG
PRIMER_LEFT_3=842,22
PRIMER_RIGHT_3=1005,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=60.352
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.4353
PRIMER_LEFT_4_PENALTY=0.198902
PRIMER_RIGHT_4_PENALTY=0.236361
PRIMER_LEFT_4_SEQUENCE=AGATGGAGAAGGAAAGAGAGGG
PRIMER_RIGHT_4_SEQUENCE=CAGCAATGCGATCTATACAAGG
PRIMER_LEFT_4=829,22
PRIMER_RIGHT_4=1010,22
PRIMER_LEFT_4_TM=60.199
PRIMER_RIGHT_4_TM=59.764
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_05153 Ps_contig_mira-and-tgicl-ass_franssen_36656
SEQUENCE=CAAATACAACAAAAGAAATACCTCCCACGGAAAGTTTAGTCTGGCGATCAAGCCAATGGTCGTTTCTTTGAACTGAAACAGAAACACTGTCTGTCTGTCTGTCTTTCTTTTGACAAATGGTAAAACTTAAGCCAGGAATGCTCAGGTCTCTGCAAAAGACACAGCAATCTTCTGGCTAGCAAATTTTAAATTTCTTCTAGTAACAATCTTCAAATATCCACTGACTTTACTGCAACCTGTTTATCATGTCTCATGTGTACATGTGATGATCCAGTGTGTAGGCACGAATATCAGAAGGACTTGTACCCGGTCTTTTGTTAATGGTTCCATAGTGAAATTTGGGTTTGCCAGCCTTGATATCTTCAACATACAAACTCAAGGTAACCACCATTGATGAAAAGGATAGAGAATTAAAAGGTATATCATCAAGTGAAAAAAGTTGGCATTCTGATGATTCTGGACCGGGTGAAAAATGGGGCTTCTTCAGCTTTGCTAAAAATATCATGTATGTTTGGCCAATTAAAGGAATGTCCAATTGAGCAAAGGGAGAAATTACTTCTACATCTGCATTTGCTTCCTCCCTTGTTTCCCTTACAGCACCTTCCATAGCTGACTCTCCGATTTCCAAATAGCCAGCGGGAAGCGTCCAAAGACCATGAGATGGTTGAATATTCCTCTTGCAAAGGAGGACTTTATTATCATGTTCAATGAGGCAACCAACTACCATTTTGGGATTTTGATAAGCAATCCTCTCACAAACTGTACATATGGCTCTCAATTTTTCTTCACCGTCAGGTATATCATGTTTAGTTGAGCCACCACACCACTGACAGAACTTGATACTTCGAACATTTCCAGAAGATTGAAGCGACGATGAAGCACGAAACGGTGCGAATCGAGTGTTGCGAGGGGTGGGAAGGAGTTTGAAAGAAGGGCTAGGGTTAGGGTTTGAAATTGGGGAAAGTGATGATAGTGATGAGAATGAAAGGGAGGTTCGAGGTTTTAGAGTCCAGCGTTGGGTGACAAACCCGCATAGCTGAAGCTGGATAGGTTTGACCATCTATTTTTCTTCTTCTTTTTCTTCTCTATCAATGGAGTTTCTGAAGTACAGAACAAACCATATCCNCCCCC
TARGET=87,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1402
PRIMER_LEFT_PENALTY=0.080887
PRIMER_RIGHT_PENALTY=0.059316
PRIMER_LEFT_SEQUENCE=AAGTTTAGTCTGGCGATCAAGC
PRIMER_RIGHT_SEQUENCE=GATTGCTGTGTCTTTTGCAGAG
PRIMER_LEFT=31,22
PRIMER_RIGHT=168,22
PRIMER_LEFT_TM=59.919
PRIMER_RIGHT_TM=60.059
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=138
PRIMER_PAIR_PENALTY_1=0.1679
PRIMER_LEFT_1_PENALTY=0.080887
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=AAGTTTAGTCTGGCGATCAAGC
PRIMER_RIGHT_1_SEQUENCE=CTGAGCATTCCTGGCTTAAGTT
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=144,22
PRIMER_LEFT_1_TM=59.919
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=0.1908
PRIMER_LEFT_2_PENALTY=0.080887
PRIMER_RIGHT_2_PENALTY=0.109882
PRIMER_LEFT_2_SEQUENCE=AAGTTTAGTCTGGCGATCAAGC
PRIMER_RIGHT_2_SEQUENCE=AATTTGCTAGCCAGAAGATTGC
PRIMER_LEFT_2=31,22
PRIMER_RIGHT_2=184,22
PRIMER_LEFT_2_TM=59.919
PRIMER_RIGHT_2_TM=59.890
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=0.2167
PRIMER_LEFT_3_PENALTY=0.157415
PRIMER_RIGHT_3_PENALTY=0.059316
PRIMER_LEFT_3_SEQUENCE=CTCCCACGGAAAGTTTAGTCTG
PRIMER_RIGHT_3_SEQUENCE=GATTGCTGTGTCTTTTGCAGAG
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=168,22
PRIMER_LEFT_3_TM=60.157
PRIMER_RIGHT_3_TM=60.059
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=0.2455
PRIMER_LEFT_4_PENALTY=0.080887
PRIMER_RIGHT_4_PENALTY=0.164595
PRIMER_LEFT_4_SEQUENCE=AAGTTTAGTCTGGCGATCAAGC
PRIMER_RIGHT_4_SEQUENCE=CAGGTTGCAGTAAAGTCAGTGG
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=238,22
PRIMER_LEFT_4_TM=59.919
PRIMER_RIGHT_4_TM=59.835
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_05154 Ps_contig_mira-and-tgicl-ass_franssen_36659
SEQUENCE=ACCATGCAATTTTTAGCAAAATTACATATCGAGGAATAAGTGGTCTAAGAATTCAATAGTGGTGGCACAAAAAGATGCCAAAATAAATAGTTGAGCTGCACTTATATTCAGTTTACACAACTACACTATTCAAAATTCAAAAACACTTACACTTCCGTTATTGACTAAACTCATCCTTGCAGAAGAAAGTTCCATAAGATACAACAATTTAACATCAATCTAGCCCAATACCAAACCAGAATGACTAAAATATAGCTATGATCCTCCACCCAAAATGTATAGGATCATATCATTAGTACCTTTGAAGAAAATTGACTTTCATCCCATCAAATCATACTGTTTATTCCCAATTAGGATCTTGAGAGGGACACTCGGTACTCACTCACAGGAACTCGTGCAATCGGATAGCATGGAAATGAATTTGAACCGAAATAAGCCTTGCCATTTCATAACCAAGTCTGAAAGGGTGGCAATTCTAGCTGTATTATATTAAAGCAGAGACTGGTTCATATCCATCTGCCTCAATTGCGTCTACCATAGATAGGTCGCCTGTCCTTGCAATTTTACCGTTGTCCATAACATGGACATGGGAAGGTTTCAAAAGATCAAGAATCCGCCGATAGTGAGTAATCATCAACAAAGAATTCTCGGGGGTAAGAATTTTATTAACAGCACTTGCCACATCCCTGAGCGCATCAACATCCAATCCCGAATCAATTTCGTCCAAAATAGCCAAATCTGCCCCCAAAACAGCAAGCTGCAAGATTTCATTGCGTTTTCGTTCACCTCCACTAAATCCTTCATTGACATTCCTATTGAGAAAGTCAGGCTTCATGTTCACAAGTTGTAGTTTCTCCATCAGATAAGAAAAACACTCAAGGGGTCCAAGTTCAGGGAGACCAAGTTTTTTCCTCCTGGCATTATAAGCCATAACAAGAAATTGATCATTCGAAACCCCCGGAATCGCAACCGGAGATTGAAAGCTCATGAAAAGACCTTCAAGCGCTCTTTCCTCCGGTTCCATTTCAAGCAAATTCCCCCCTTTGAACACAACACTCCCACCAGTGACTTCATAATCTGGATGACCAACAAGAACCTTAGCGAAAGTGCTTTTTCCAGAACCGTTTTTCCCCATGATAGCGTGAACCTCGCCTCTGTTGACAGTGAGATTAACGCCTTTGAGAATTTCGAGATTGGATTCTACGATTTTGGCTCTTAGATCATTGACTTGAAGGAGAGGAATCTCGGTGCTCGCCGTCGCCGCCGCGACGCAAGGGAAATTGCGGCGAGTTTGGGTTTTGAGAGAAAAACGGAATTGAAATGGAAGAGATAGCGATAGTGATGATGATGATGAAATAGCCATCACT
TARGET=1340,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05155 Ps_contig_mira-and-tgicl-ass_franssen_36661
SEQUENCE=GGTAGTCAACGCAGAGTANTCGGGGGGTAGCTAAAATTCTAACTTCTAAAAACAGTTGAATACTGAAAAATGGATAAGCTACTATGGAGGGTATTGCCTTTATCAACGCTCCTAATTTCCTTCGCACTTTTCTTCTACCTACACAATTCCAACAACGCGGAACTATATCCTATTATTAAGTTAGCAGAGGGTGAGTGCAGTCTTTTTCCGTATGATCATTACTGGATTTCTAGCAAGCGGATTTTGACTTCACAAGGAATCATTTCTGGATCAGTGGAGATAAATGAAGGGAAAATAGCATCTATTGTGGAAGGATATGGCAAGCAGGGGAGTTCTGTTCAACAAGTGATTGATTATGGAGATGCTTTTATCATGCCTGGCTTGATTGATGTGCATGTACATCTTGATGAGCCCGGCAGAACCGAATGGGAGGGATTTGTCACTGGTACCAGAGCTGCTGCTGCTGGAGGTGTAACTACAGTGGTYGACATGCCTCTAAACAATCACCCAACAACCGTGTCTAAGGAAACACTGGAACTGAAGCTTGAAGCTGCAGAAGAATAAATCTATGTTGATTGGTTTGGGTTTTTGGGGGAGGTCTTGACTTCCCTAAGNAANGACRACAAAATCCAGTATTCTTAGGAAGGTTCTTACG
TARGET=454,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05156 Ps_contig_mira-and-tgicl-ass_franssen_36662
SEQUENCE=ACGACAANNTNTATTTCAAATTTATTATATATAAAGATAAAGGTCCACTACATGACATTTAAAATTGAAACCAAAATGATATACTGGGCGAGAGCTAGAATAGATACGCCTATAATTAACAACATTTTATTGATTGAAATTAACTGCAAAAGGCAATTTAAGATGTTCATTGAAAAATACTACTGACAGAACTATCATCATGAATTCTCCAAATAGTTTCACCCAACAGGTTTGCTACGGTAAGAATAGTTAACTGAGGGAAATAGTTCTGCTCTGAAACTGGAATGGTGTTGGTAATTATCACCTCTTGAAATAAGCCACTGGATAGCCTCTCAATTGCAGGAGGGCTGAAAACTCCGTGAGTGCAACAAGCATAAACTTCCCTAGCTCCCTCTTCATGTAATAGTGCTGCACCCTTGGCAATGGTTCCAGCAGTGTCAATCATATCATCCACCATTACAGCAACTTTTCCTTTTACATCACCAATCAGATTCATCACCTCAGCTACATTGTGTCCGTGGCGCCTTTTGTCTACAATAGCCAAAGGTGCATCAGATAACTTCTTAGCAAAAGCACGTGCTCTTGCAACACCGCCAACATCAGGGGAAACAACCACCAAGTCATTCGAACTTATTGTCTTACTAGCAAGATAATCGAGGATCACAGGCTGACAATGCACATGATCCACTGGAATATCAAAGTAACCCATGGACTGCCCAGAATGAAGATCACAAGCAAGAACACGATCGGCCCCAGCTTTGGTAATAAGGTTCGCAACAAGTTTAGCTGCAATTGATTCACGCCCTTGAGTCTTTCTATCAGCTCTAGCATATCCAAAATACGGAATGACAGCAGTGATGTTTTTGGCGGAAGCTCTTCGACAAGCATCGATCATTATCTGAAGCTCCATGAAATTCTCATTTGCAGGAGGGCATGTTGGTTAATAAGGTATACATTGCAACCTCTAACACTCTCTTGTAACTGGACATAGATTTCACCATCTGCAAATCGCTTTATCGAAACTTTTCCAAGCTCCAAACCCATATACCGAGCAATTTCCTGAGAGAGTGCAGGATTAGCGGTACCGGAGAACAATTTCAACTTGCTACCGTTTCTACTAACGGTGTTCTCAGTTTTTGCAGATTCCATGAACTTGGGTAGAACGGGAACAATCGGTTTCCCGTTNAGCAAATTCGAAGCTTCAGACATATCACATTTCACGCTATTGTACGCGACGGTTCGAGTGCGGGGAAGGTCAAGAAAACCTAATGTTCGAACGGTGGAGGCATGAGAGGCGCCGGTGAAGAGAGAGGAGGAAGAAGAAGTGAAAGGCATGGTTGATGACGACGTGTGTTGAACTTGAAAAAGTGATGAAGCCATGTGTTCAGGAACAACACGTGTCACGGTTAGGGTTTGTGTTCTCTGCAACAGAGAGAGAGGGAAGTTTGAAGCAGAACAGAGCGATTCTGGAGCTCTGTTGTTGTTTGTGGCGGGTCTGTNNNCCGAC
TARGET=1430,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4677
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.465411
PRIMER_LEFT_SEQUENCE=GAGGAAGAAGAAGTGAAAGGCA
PRIMER_RIGHT_SEQUENCE=CAAACAACAACAGAGCTCCAGA
PRIMER_LEFT=1312,22
PRIMER_RIGHT=1486,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.465
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=175
PRIMER_PAIR_PENALTY_1=0.4698
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.465411
PRIMER_LEFT_1_SEQUENCE=AGAAGTGAAAGGCATGGTTGAT
PRIMER_RIGHT_1_SEQUENCE=CAAACAACAACAGAGCTCCAGA
PRIMER_LEFT_1=1320,22
PRIMER_RIGHT_1=1486,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=60.465
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.5462
PRIMER_LEFT_2_PENALTY=0.080808
PRIMER_RIGHT_2_PENALTY=0.465411
PRIMER_LEFT_2_SEQUENCE=GATGACGACGTGTGTTGAACTT
PRIMER_RIGHT_2_SEQUENCE=CAAACAACAACAGAGCTCCAGA
PRIMER_LEFT_2=1339,22
PRIMER_RIGHT_2=1486,22
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.465
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=0.5588
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.556510
PRIMER_LEFT_3_SEQUENCE=GAGGAAGAAGAAGTGAAAGGCA
PRIMER_RIGHT_3_SEQUENCE=ACAAACAACAACAGAGCTCCAG
PRIMER_LEFT_3=1312,22
PRIMER_RIGHT_3=1487,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=59.443
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=0.5609
PRIMER_LEFT_4_PENALTY=0.004359
PRIMER_RIGHT_4_PENALTY=0.556510
PRIMER_LEFT_4_SEQUENCE=AGAAGTGAAAGGCATGGTTGAT
PRIMER_RIGHT_4_SEQUENCE=ACAAACAACAACAGAGCTCCAG
PRIMER_LEFT_4=1320,22
PRIMER_RIGHT_4=1487,22
PRIMER_LEFT_4_TM=60.004
PRIMER_RIGHT_4_TM=59.443
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=168
=
PRIMER_SEQUENCE_ID=SSR_05157 Ps_contig_mira-and-tgicl-ass_franssen_36666
SEQUENCE=CTTTCCATTTCTATGGCATCATCTGAGAACAATGATACAAGTTTAGTAGATATTGATTTGGAGGCGTTTGAAATTGATGTAAATCTCTTAAGAGAATTGCTTGAAGAAGAAGAAGAAGACGGGGAAGGGTGCAAAAGATAGTGGTAACAAAGAATGCGTAGCAGAATCCCTAGAGGAAAACAGAAATGTTAATCCTAGTATGATGAAGGAGGAGAAAGAGGGGCAGCAACAGAGTTGTTTGGAGAAGAATGAGTGTCATTATGTTCATGATTTTGAGTGGCTGAATATGATGGACTTGATGGAGCCAACCAATCCATTGGATGAAGTGATGACAACGAATTGGTTTTCAGATGACAATGGGAAGTTTGATTTTGATTTTGGTTATGCTAATGGAGAATGCTACCCTCAAACTTGCGATGGTATTGTCTCCTATGACTCGAATTATAGTCGTTTGTGGGGAGATTGTTGATATTTAGATGCTTATCAATGCTCAAAAGAGGAGGGGGGAAATTGCTTATCAAAATTTAGCTACTGGTAATATCAATAATGAGAGGGGAAGGAGAAAATAGTAAAAGTAACTGAAGTGTAAAAGAATCTATTTTAATCATCTATTTTATAATTAGTAAGTTAAAGAGTATAATTACAGCTTTTAGACTCCACCAAGTTATGTCTTGATATATAGTAGTA
TARGET=103,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0759
PRIMER_LEFT_PENALTY=0.037936
PRIMER_RIGHT_PENALTY=0.037936
PRIMER_LEFT_SEQUENCE=ATGGCATCATCTGAGAACAATG
PRIMER_RIGHT_SEQUENCE=GTCCATCATATTCAGCCACTCA
PRIMER_LEFT=12,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=59.962
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.0781
PRIMER_LEFT_1_PENALTY=0.037936
PRIMER_RIGHT_1_PENALTY=0.040146
PRIMER_LEFT_1_SEQUENCE=ATGGCATCATCTGAGAACAATG
PRIMER_RIGHT_1_SEQUENCE=CCATCATATTCAGCCACTCAAA
PRIMER_LEFT_1=12,22
PRIMER_RIGHT_1=292,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=59.960
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=0.1153
PRIMER_LEFT_2_PENALTY=0.077340
PRIMER_RIGHT_2_PENALTY=0.037936
PRIMER_LEFT_2_SEQUENCE=TCCATTTCTATGGCATCATCTG
PRIMER_RIGHT_2_SEQUENCE=GTCCATCATATTCAGCCACTCA
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=59.923
PRIMER_RIGHT_2_TM=59.962
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=292
PRIMER_PAIR_PENALTY_3=0.1396
PRIMER_LEFT_3_PENALTY=0.037936
PRIMER_RIGHT_3_PENALTY=0.101680
PRIMER_LEFT_3_SEQUENCE=ATGGCATCATCTGAGAACAATG
PRIMER_RIGHT_3_SEQUENCE=CTCTAGGGATTCTGCTACGCAT
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=10.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=163
PRIMER_PAIR_PENALTY_4=0.1558
PRIMER_LEFT_4_PENALTY=0.037936
PRIMER_RIGHT_4_PENALTY=0.117817
PRIMER_LEFT_4_SEQUENCE=ATGGCATCATCTGAGAACAATG
PRIMER_RIGHT_4_SEQUENCE=TCAGCCACTCAAAATCATGAAC
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=283,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=60.118
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_05158 Ps_contig_mira-and-tgicl-ass_franssen_36668
SEQUENCE=GCGANGGGGATGAATCAGCCGCAGCATCAGCAGCAGCAGCATCTGTTCCAAAATCTCTGATGGAGAATCTGTTAGGTCTTTTGAGGGTTCGTATCAAACGTGGCGTCAATCTTGCTGTTCGTGATGTTCGTAGCAGCGATCCTTATCTTGTCATCAAGATGTATAATCAGAAACTTAAGACTCGTGTGATTAAAAGGGATGTTAATCCTGAGTGGAATGAAGATCTTACTCTTTCTGTTATAGATCCTAATCACATAGTTTCATTGACTGTGTATGATCATGACACTTTTAGCAAGGATGACAAAATGGGAGACGCGGAATTTGACTTCTCTTCTTATATAGAAGCCCTGAGGATGGACGTAACCGGCCTTTCAAACGGCACCATACTCAAACGAATTCAACCAAGTAAGCACAACTGCCTTACTGAGGAGAGCCGTATCTATTATAACAGTGGAAAGATTATCCAAGATATGATTCTTAGATTGCAAAATGTGGAATGTGGTGAAGTTGAAATCAGTTTGCACTGGATTGATCTTCCTGGTTCTAAAGGTTTATGATTTTATATATCAGATGTTTGTTTTTGAGCAACTTTATATATATAGATTTATATATACATATAAAAGGTGAATTATACATTCAACTTTGTGAAGTACTATTATAGTTTGGTTTTATGTAAGAGTTTAGTAACAAAATGAACTTGAACTTATTCATCAAGTCTTATATTTGTATCCAGTTTATAATGTATTTAGTACAGTTCAAAATTYYATTTTTCTRAGTYACA
TARGET=28,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05159 Ps_contig_mira-and-tgicl-ass_franssen_36668
SEQUENCE=GCGANGGGGATGAATCAGCCGCAGCATCAGCAGCAGCAGCATCTGTTCCAAAATCTCTGATGGAGAATCTGTTAGGTCTTTTGAGGGTTCGTATCAAACGTGGCGTCAATCTTGCTGTTCGTGATGTTCGTAGCAGCGATCCTTATCTTGTCATCAAGATGTATAATCAGAAACTTAAGACTCGTGTGATTAAAAGGGATGTTAATCCTGAGTGGAATGAAGATCTTACTCTTTCTGTTATAGATCCTAATCACATAGTTTCATTGACTGTGTATGATCATGACACTTTTAGCAAGGATGACAAAATGGGAGACGCGGAATTTGACTTCTCTTCTTATATAGAAGCCCTGAGGATGGACGTAACCGGCCTTTCAAACGGCACCATACTCAAACGAATTCAACCAAGTAAGCACAACTGCCTTACTGAGGAGAGCCGTATCTATTATAACAGTGGAAAGATTATCCAAGATATGATTCTTAGATTGCAAAATGTGGAATGTGGTGAAGTTGAAATCAGTTTGCACTGGATTGATCTTCCTGGTTCTAAAGGTTTATGATTTTATATATCAGATGTTTGTTTTTGAGCAACTTTATATATATAGATTTATATATACATATAAAAGGTGAATTATACATTCAACTTTGTGAAGTACTATTATAGTTTGGTTTTATGTAAGAGTTTAGTAACAAAATGAACTTGAACTTATTCATCAAGTCTTATATTTGTATCCAGTTTATAATGTATTTAGTACAGTTCAAAATTYYATTTTTCTRAGTYACA
TARGET=592,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05160 Ps_contig_mira-and-tgicl-ass_franssen_36675
SEQUENCE=TCGGGGGTTAAAAATAAATAAATCATTGGGAAAGTATCGTCAAGTTTGAACATCTGAAATCTGAATTCCTTGTTCAGGAAAACGTGTCCACATAAAACAAAAAGCAAACTATAAGCAACCAAGCTTCTCCTCCTGATCCATCTTCTTCCACATCTTTCTTCTTTCGATCCAAATCCCTAGATTCAGTTTCAATCTTCAACTCATCCTCTCTCTCTTCAAATCCTCCATTCACAACAACAATGGATCTAGCTCCAGAAGAACTCCAATTTCTCACCATCCATAACATCTTAACCGAATCAATCTCAATCCCTAAAACTTCCCCTAAAACCTTCTACCTCATTACCCTAACCCTAATTTCCCTCTTTCTTTCGCAATTCTAGCTCATTCCCTTTTCACACATCCCTTAATTTCACATCTTCAATCTCCTTTCACTGATCCCTCTCAAACCTCTCATGATTGGACTCTTCTCCTCGTTTTTCAGTTCTTTTACCTCATTTTCCTCTTCGCTTTCTCTCTCCTCTCCACCGCTGACCGTTAGTTTTCACCGTCGCGTCACTCTACACCTCAAAACCGGTGTCTTTTTCGAGCACTTTCCTGACTCTCATCCTCTTCTCTTCCTCGGTGATCTCTTCATCTCCATCATCGATTTTTTCACTCTGCAAATACTCTTCCGTTTTCTTCTCAAGCTCTCTCCTCTCCTCAAGCTCCCTCACTCTCCTCTCCTCAAGCTCCCTCACTCT
TARGET=12,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05161 Ps_contig_mira-and-tgicl-ass_franssen_36675
SEQUENCE=TCGGGGGTTAAAAATAAATAAATCATTGGGAAAGTATCGTCAAGTTTGAACATCTGAAATCTGAATTCCTTGTTCAGGAAAACGTGTCCACATAAAACAAAAAGCAAACTATAAGCAACCAAGCTTCTCCTCCTGATCCATCTTCTTCCACATCTTTCTTCTTTCGATCCAAATCCCTAGATTCAGTTTCAATCTTCAACTCATCCTCTCTCTCTTCAAATCCTCCATTCACAACAACAATGGATCTAGCTCCAGAAGAACTCCAATTTCTCACCATCCATAACATCTTAACCGAATCAATCTCAATCCCTAAAACTTCCCCTAAAACCTTCTACCTCATTACCCTAACCCTAATTTCCCTCTTTCTTTCGCAATTCTAGCTCATTCCCTTTTCACACATCCCTTAATTTCACATCTTCAATCTCCTTTCACTGATCCCTCTCAAACCTCTCATGATTGGACTCTTCTCCTCGTTTTTCAGTTCTTTTACCTCATTTTCCTCTTCGCTTTCTCTCTCCTCTCCACCGCTGACCGTTAGTTTTCACCGTCGCGTCACTCTACACCTCAAAACCGGTGTCTTTTTCGAGCACTTTCCTGACTCTCATCCTCTTCTCTTCCTCGGTGATCTCTTCATCTCCATCATCGATTTTTTCACTCTGCAAATACTCTTCCGTTTTCTTCTCAAGCTCTCTCCTCTCCTCAAGCTCCCTCACTCTCCTCTCCTCAAGCTCCCTCACTCT
TARGET=206,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1078
PRIMER_LEFT_PENALTY=0.033905
PRIMER_RIGHT_PENALTY=0.073866
PRIMER_LEFT_SEQUENCE=TCTTTCGATCCAAATCCCTAGA
PRIMER_RIGHT_SEQUENCE=ATGAGAGGTTTGAGAGGGATCA
PRIMER_LEFT=159,22
PRIMER_RIGHT=453,22
PRIMER_LEFT_TM=60.034
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=0.1078
PRIMER_LEFT_1_PENALTY=0.033905
PRIMER_RIGHT_1_PENALTY=0.073866
PRIMER_LEFT_1_SEQUENCE=TCTTTCGATCCAAATCCCTAGA
PRIMER_RIGHT_1_SEQUENCE=TCATGAGAGGTTTGAGAGGGAT
PRIMER_LEFT_1=159,22
PRIMER_RIGHT_1=455,22
PRIMER_LEFT_1_TM=60.034
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.1440
PRIMER_LEFT_2_PENALTY=0.033905
PRIMER_RIGHT_2_PENALTY=0.110131
PRIMER_LEFT_2_SEQUENCE=TCTTTCGATCCAAATCCCTAGA
PRIMER_RIGHT_2_SEQUENCE=AGGTTTGAGAGGGATCAGTGAA
PRIMER_LEFT_2=159,22
PRIMER_RIGHT_2=448,22
PRIMER_LEFT_2_TM=60.034
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.1497
PRIMER_LEFT_3_PENALTY=0.075795
PRIMER_RIGHT_3_PENALTY=0.073866
PRIMER_LEFT_3_SEQUENCE=CGATCCAAATCCCTAGATTCAG
PRIMER_RIGHT_3_SEQUENCE=ATCATGAGAGGTTTGAGAGGGA
PRIMER_LEFT_3=164,22
PRIMER_RIGHT_3=456,22
PRIMER_LEFT_3_TM=59.924
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.1497
PRIMER_LEFT_4_PENALTY=0.075795
PRIMER_RIGHT_4_PENALTY=0.073866
PRIMER_LEFT_4_SEQUENCE=CGATCCAAATCCCTAGATTCAG
PRIMER_RIGHT_4_SEQUENCE=ATGAGAGGTTTGAGAGGGATCA
PRIMER_LEFT_4=164,22
PRIMER_RIGHT_4=453,22
PRIMER_LEFT_4_TM=59.924
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_05162 Ps_contig_mira-and-tgicl-ass_franssen_36678
SEQUENCE=AANTAATTTAANTCAAAACCCTACTCAAGGAAACTCAACAGCTCCATCAAAACGACACTGCATTTCGCACTGAACTGAATTCGCCGGAAAACGCAGCCGGAGCTCGTCGTCGGCCGCCTTAAACTGTGAGAAGGATTTGTGCTGGAATAGAGTTCGCAATGTACGTTGAATGAGCTGATTTGCCAATTCAGAGATCTTTACAAGCCAAGAATGAGCTTGCTTTTGAGAATAAGACATTGTTTACCCAATGGTTTGTATAGGCAACCTCTTTATGGACGCATAGTAGGTTTGAATGCTAGGAAATTAAATGTATTTTCAAGAAACCTTGGTCAAGCGGCGAGAAAAGAGGAGGAGGAGGACGTAGAGGAAGTGGAAATTGATCAGAGAAGTCTCCCGGCTGATTTTGATCCTGCAACATTTGATCCCAATGAGAATCGCGGCCCTCCATCAGAGAGAGTTTTCAGGCTTGTTGATGAAGTGGCGTCTCTAACGCTAGCTGAAGCTGCAGAATTGGGTCTTGTTCTGATGAAGAAAATGGGTATAAAGGAGATGCCTAATGTGGGATATTTGAAAGCCGGGACTGCAAATTTGGCTGGAATGGCAGCAAAAGCCTCGGCACCAGTCAAAGAGGAGGTAAAACCAGAAAAAACTGTATTTGAATTGAAACTGTTGTCCTATGAAGCAGCTTCCAAAATTAAAGTCATCAAAGAGGTCCGGGGCTTTACGGATTTAGGTTTGAAAGAGGCAAAGGATCTAGTGGAAAAAACACCTTCTATTATAAAGAAAGGCGTTTCAAAGGTAGAAGGAGAACAGATAATAGAGAAACTGAAAGCACTTGGTGCAAATGTTGTTATGGAGTGAAGTAGTATTGTGTTTCTTAGAGATATGTTTCTTCCAATATTCAAAAGTTACCTGAAATTTAGTAAATTTTGTAATGTTGACTGAGGATACTGCAAATTTTTGTAGCGTTGTTGTCTACGATGTTTCAATCATTTTCTGCTGCGATGAAACAATATCCCTCCGATGGAATAAAAGTAACATTAGATACCTCTTATAGTGATAACT
TARGET=346,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7539
PRIMER_LEFT_PENALTY=0.470203
PRIMER_RIGHT_PENALTY=0.283676
PRIMER_LEFT_SEQUENCE=CCCAATGGTTTGTATAGGCAAC
PRIMER_RIGHT_SEQUENCE=CACTTCATCAACAAGCCTGAAA
PRIMER_LEFT=243,22
PRIMER_RIGHT=479,22
PRIMER_LEFT_TM=60.470
PRIMER_RIGHT_TM=60.284
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.7590
PRIMER_LEFT_1_PENALTY=0.470203
PRIMER_RIGHT_1_PENALTY=0.288804
PRIMER_LEFT_1_SEQUENCE=CCCAATGGTTTGTATAGGCAAC
PRIMER_RIGHT_1_SEQUENCE=TCTTCATCAGAACAAGACCCAA
PRIMER_LEFT_1=243,22
PRIMER_RIGHT_1=531,22
PRIMER_LEFT_1_TM=60.470
PRIMER_RIGHT_1_TM=59.711
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=289
PRIMER_PAIR_PENALTY_2=0.7590
PRIMER_LEFT_2_PENALTY=0.470203
PRIMER_RIGHT_2_PENALTY=0.288804
PRIMER_LEFT_2_SEQUENCE=CCCAATGGTTTGTATAGGCAAC
PRIMER_RIGHT_2_SEQUENCE=TTCTTCATCAGAACAAGACCCA
PRIMER_LEFT_2=243,22
PRIMER_RIGHT_2=532,22
PRIMER_LEFT_2_TM=60.470
PRIMER_RIGHT_2_TM=59.711
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.7607
PRIMER_LEFT_3_PENALTY=0.477034
PRIMER_RIGHT_3_PENALTY=0.283676
PRIMER_LEFT_3_SEQUENCE=ATAGGCAACCTCTTTATGGACG
PRIMER_RIGHT_3_SEQUENCE=CACTTCATCAACAAGCCTGAAA
PRIMER_LEFT_3=256,22
PRIMER_RIGHT_3=479,22
PRIMER_LEFT_3_TM=59.523
PRIMER_RIGHT_3_TM=60.284
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.7658
PRIMER_LEFT_4_PENALTY=0.477034
PRIMER_RIGHT_4_PENALTY=0.288804
PRIMER_LEFT_4_SEQUENCE=ATAGGCAACCTCTTTATGGACG
PRIMER_RIGHT_4_SEQUENCE=TCTTCATCAGAACAAGACCCAA
PRIMER_LEFT_4=256,22
PRIMER_RIGHT_4=531,22
PRIMER_LEFT_4_TM=59.523
PRIMER_RIGHT_4_TM=59.711
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_05163 Ps_contig_mira-and-tgicl-ass_franssen_36679
SEQUENCE=CCGAAAGATACTTACTACTTAGTATCGGCTAAATATGTTACATAGTACCAAGGGAAATGGATATTCATAGTAGACACCATTATCTTTGTTAGCATTAGAATATATAACTTCATAAACATCAGCATTTTGCTCAGCTCAGCTCAGCTCCATCATTTGCACATAGTTGTCTTCAACCTCTTTGCAGCTTTGACAAATCCTCGAATAACTTGCAACTCGTCCATTTGCTGTGACATGAAATGCCTCTGAACAACATCTACCAGCTGCTCTTTGGACGGGTTTGGAACAGCATCCACCAGATTGAAGTGTCGCCAATATCTCCACAATGCAGCCATTTTCCTAATTTTGTCTCAAATCAACCGTCCACCCATGAGGCATGGAAATRGATCCCTTGGATTGTGAATCAANNGAAAA
TARGET=129,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05164 Ps_contig_mira-and-tgicl-ass_franssen_36682
SEQUENCE=TATCTATCTGGTCTTAACTTGATTTGAGCCAATGAATGAATGAAATTATGTGTTTGTTTGATTTGTGCAGTTTGTAAATCAAATGTTGGAAATAATTCGGTGGATTTTCCATTGCTTGTTCCAAATGGGACTTATTTCTATACTGCAAATGATTGTGTTAAATGTAAATGTGACTCTACTGGTAGTAATAACAACAGCAATTCTATGTTGCAATGCGAGGAGTCGAATATAAAACCGATAAATCATTGGTCGGTTTGTCCGTCTGCGAAATGCTCTGGGAATGTGTTGATTGGGAATACTACGTCTACTGATTCGTGTAATCGGACATTTTGTGATTACGCTGGTTATACTTCTCGCAATATCTCTACGGTTCTTGCAACTCAAAACACTTGTGCTGTGGCGCCAAGTGGTTCGAGCGGTTCGGGTTCAGGTGGTTCGGATTCAGGGGCATCTAGGAGTATCTTGAATGGCTGGGTTTGGAATAAGCTTCTTATTCTCATTCACTTCCTTCTGTTTTTTGTTTATCTTTTGTAGTACAAACCCTGCATCTTTGTTCTTTTTCTTTTCATGAGTTCTTTGTTTAGTGTTGTGTCTTGTGAGTGTTGCCACTTAATTGAATATTTTTATGTTTTTTTCCTTCTATTTAGTGCATGTTTGGATTGATTGTGAGTTAGGCTTTGTCAGAGTGCTACCATGACTTTGTCATACTCACATTGATTTCAAAGATACAATTGCAATATTTGTAATATACGAGCATGTTTGGAAGAATAAGAATATTGTCTGAACTACAATAATCTGTATCTTTTTTAGTGTTAGCTTCTGTTGAGTCATGTTAA
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6385
PRIMER_LEFT_PENALTY=1.622174
PRIMER_RIGHT_PENALTY=0.016290
PRIMER_LEFT_SEQUENCE=GGTCTTAACTTGATTTGAGCCA
PRIMER_RIGHT_SEQUENCE=CGGTTTTATATTCGACTCCTCG
PRIMER_LEFT=9,22
PRIMER_RIGHT=236,22
PRIMER_LEFT_TM=58.378
PRIMER_RIGHT_TM=59.984
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=2.2458
PRIMER_LEFT_1_PENALTY=1.622174
PRIMER_RIGHT_1_PENALTY=0.623595
PRIMER_LEFT_1_SEQUENCE=GGTCTTAACTTGATTTGAGCCA
PRIMER_RIGHT_1_SEQUENCE=ATAAGTCCCATTTGGAACAAGC
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=134,22
PRIMER_LEFT_1_TM=58.378
PRIMER_RIGHT_1_TM=59.376
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=2.2610
PRIMER_LEFT_2_PENALTY=1.622174
PRIMER_RIGHT_2_PENALTY=0.638796
PRIMER_LEFT_2_SEQUENCE=GGTCTTAACTTGATTTGAGCCA
PRIMER_RIGHT_2_SEQUENCE=CTCGCATTGCAACATAGAATTG
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=218,22
PRIMER_LEFT_2_TM=58.378
PRIMER_RIGHT_2_TM=60.639
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=2.2700
PRIMER_LEFT_3_PENALTY=1.622174
PRIMER_RIGHT_3_PENALTY=0.647803
PRIMER_LEFT_3_SEQUENCE=GGTCTTAACTTGATTTGAGCCA
PRIMER_RIGHT_3_SEQUENCE=GCATTGCAACATAGAATTGCTG
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=215,22
PRIMER_LEFT_3_TM=58.378
PRIMER_RIGHT_3_TM=60.648
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=2.3336
PRIMER_LEFT_4_PENALTY=1.622174
PRIMER_RIGHT_4_PENALTY=0.711395
PRIMER_LEFT_4_SEQUENCE=GGTCTTAACTTGATTTGAGCCA
PRIMER_RIGHT_4_SEQUENCE=GAACAAGCAATGGAAAATCCAC
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=120,22
PRIMER_LEFT_4_TM=58.378
PRIMER_RIGHT_4_TM=60.711
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=112
=
PRIMER_SEQUENCE_ID=SSR_05165 Ps_contig_mira-and-tgicl-ass_franssen_36686
SEQUENCE=TTAACAAAATACTTCTAAATTTTTATTTTTANTTAACATTTACTGACTAATCACTAGCTAGTAAAATGATGCAAATTAACCAACACCAACACCAAGACTTTTGAATTTTATTTTGAACTCAAAATACAAAATCTTCAACCAAATTGTTTTTCTTCTCATTGTTTTCTAGTCAACGATAAAGTATGATAGATGAACTTAGTTCTCTCTTTGGACATTCGATCAGTTAAAATATTTTTATAGCGTTCAAAAATCTCTTCAATGACACTATCACCAAAGTGACAAACTAGCAAAGGTTCAGTCCCAGCCCTAATGCATTGTGCCATATTGTATCCTTCGTCTTTGAACGATTTCGACATTTCAGATCCAATGTCCAAAGCTTCCCAACTATCACGACCATTCCAATTCACTTCAGAAATCTCTAGTTTATCGATTGCAAATGATCCTTCCTCGAGAACTTCAAAATTCACTTCAGATGGAGAAGGAAAGTAGTTTGGGATGTTGAAAGTATCGAGTTTCTCTTCGTCAATGATTCCCTGCAAGACCATGTCCTTGAGAGCAATTGTCATGAGATCCCAAACTTGACAACAGTCTTTGTTTGATGGATCATCATTTTTTCTTCCTACAAATGTAAGAATCATGCGACCACCTTCAACTATTTCTTGTGCGCGACACTCGAGAAAAAGAGAAAAATCTTTTTGAAATTGCTTGTAGTAAGCCTCGAGGACATTTGAAGGGCTTGTAGTTGACAAATAAATATTACCCTTGTTGTTGTTGTCAACACCCTCTGGAACCTTTGATAGCCAATGAAGACAGGAAGAGGAATGAACAAAATCCATACTTTTATCTGCGAAAATCCTGCCATAAAATGAACCAGGAATCCCAGAGAAGTAGCAAGAACCCATTTTAGTTTCCAATTCATCGACTAGTTTTTCCTTAAAGGTGTCAAGGGACTTAAATACACTGTTGAAGTCGTTTCCGGGTAAATCATTAAGATAGACTTTATATTCAGGAGATTTCTGATTAAATTTTCGACAAAGATTTTCCACAACCTTGATAGTTTGTGAGATGACTAACAAAGTGTTTGGTCCAGAAGAACAACCTAAGTCAGCAATGGCCAAGCTTTTGGGCAGTGTGTTGCAGTACAAACTTGTTATGGCTTCATCTCTCAGAGCTTTTGTCAAAGAAATCACCTTTCGCTGCATATTTACAAACTTGATATGGATATTTTTGTATAATAGTTATATAGTTGCATTATTTATAAATATACCTGAAGTGAGGAGTTGTTTGCA
TARGET=763,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0834
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=0.008930
PRIMER_LEFT_SEQUENCE=TTCTCTTCGTCAATGATTCCCT
PRIMER_RIGHT_SEQUENCE=CTATCAAAGGTTCCAGAGGGTG
PRIMER_LEFT=513,22
PRIMER_RIGHT=799,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.991
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.1086
PRIMER_LEFT_1_PENALTY=0.074466
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=TTCTCTTCGTCAATGATTCCCT
PRIMER_RIGHT_1_SEQUENCE=ATTGGCTATCAAAGGTTCCAGA
PRIMER_LEFT_1=513,22
PRIMER_RIGHT_1=804,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.1480
PRIMER_LEFT_2_PENALTY=0.139083
PRIMER_RIGHT_2_PENALTY=0.008930
PRIMER_LEFT_2_SEQUENCE=AGACCATGTCCTTGAGAGCAAT
PRIMER_RIGHT_2_SEQUENCE=CTATCAAAGGTTCCAGAGGGTG
PRIMER_LEFT_2=538,22
PRIMER_RIGHT_2=799,22
PRIMER_LEFT_2_TM=60.139
PRIMER_RIGHT_2_TM=59.991
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=262
PRIMER_PAIR_PENALTY_3=0.1732
PRIMER_LEFT_3_PENALTY=0.139083
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=AGACCATGTCCTTGAGAGCAAT
PRIMER_RIGHT_3_SEQUENCE=ATTGGCTATCAAAGGTTCCAGA
PRIMER_LEFT_3=538,22
PRIMER_RIGHT_3=804,22
PRIMER_LEFT_3_TM=60.139
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.2105
PRIMER_LEFT_4_PENALTY=0.074466
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=TTCTCTTCGTCAATGATTCCCT
PRIMER_RIGHT_4_SEQUENCE=TATCAAAGGTTCCAGAGGGTGT
PRIMER_LEFT_4=513,22
PRIMER_RIGHT_4=798,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_05166 Ps_contig_mira-and-tgicl-ass_franssen_36693
SEQUENCE=AGTCAAACCGATGTTCCATGCATTTCAACACTATCAAAATGTTTTCCGATGGGTTAGCTCCATAATATGGAGATTCAGAAACTAACCTCTCAAACTCTAAACAAAGCTCAATAATATCTATAAATTATAATGAATCTAAATATTACATCACAAAATACATCCATTCAATAACCTGCAGCTAATACATTCCTCCAAGTCTGTCACGTCCCACACGCCTATCATCTCTTCCTCCAACACGGTTCATGACATTATCCCGGCGATAGTCTTTTGCTGGTGGGCCACCTCTGATGGGAGAACGGCTTCTTTCTCTTGCGTCCCGAGTCCACCGATCGCCACCGCCGCGATGGGGAGGAAGTGACGGCAAAAGAGGTGCAGGTGGTGACAGTGGCCTTCTCGGTGACAGTGGCCTTCTCGGTGACAGTGGCCTTCTCTCGAATCCTTTCCTTTCATTGGAGAAACCTTCTCTACCACGATCTCTATTATCCCAGTCCAATGGAGGTGGCCTATCAAACTTATTTCTCCCCCTATAGGCATCATCCATGTAATCCATTCGCTCTCTATGCACATTGGGTTCTGGAAACCTACCTTCGTGTCGGAGGGGTGGCAGAGGAGCAGCCGATCCATACTCTCTAGGGCCATCTCTTCCCATCAGACGAGGAGGAGGAGGAGATCTGTAGCCATTCATAGGCCTTTCTGGGGAACGGTCAATTGGAGGTCCAGGATAGCGTCTAGGAGGAGCGTGAAGTGGTGGAGAACCCCTCCTAGGAGGACTTCCAGCAACAGCAGGACGAGGCCTCTTACAGTTGTTACAAAAGTCACGCCTTGCAAAATTAAGATTACCACATAATGAGTCAGGACACATCCAATCTCCCTCCCTAGGACGCACATTGGGATTGTTTCTACCATGGCCTTCCCCTCTTGCATGACCATGACCATGACCATGGCCGTAGCGAGGCCCATCAAAAGCTCTACCAAATGGCCTGCCGCCTGCTCTACCTCGAGCATACGGGTGTGGAGGGTCTCGATATCTGCCAGGGTCTCTCCCGCCACCATATCCACGGTTCCGTGACAAGTGATTATAGTCAGAAGTGAAGCGATGATCAGCATCTCTACGGTGAACAGGAGATGAGGAACCTGCACGAAATCTATATCCAGGATCATCAGAATAGCGATCAGAGCGAGAGTAAGGAGGTGGAGGAGGATCACGGCGGAGTTCACCGGGCTCGTAATCGTTGGAATGAGCACCACCGGGTGAATTCTCGGCTAGGGAGGGGCGGACGACGAGGCTGCTCAAGAGAGGTTGGTGATGGTGAGGTGGAGATTGGTCACTGTCCCTTGAAGATCCCATGGCTGTTTTTTCTGGAGGAGAAGAAGATTCTGGATCGGTGATTTCTGAATTGTGATCCACTAGGTTCNTTTTTCGATCCCGGCGAGGATGTTTATAAACCCTAACANCA
TARGET=655,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1489
PRIMER_LEFT_PENALTY=0.033137
PRIMER_RIGHT_PENALTY=0.115769
PRIMER_LEFT_SEQUENCE=GTTCTGGAAACCTACCTTCGTG
PRIMER_RIGHT_SEQUENCE=AAGGCCTATGAATGGCTACAGA
PRIMER_LEFT=570,22
PRIMER_RIGHT=691,22
PRIMER_LEFT_TM=60.033
PRIMER_RIGHT_TM=60.116
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=122
PRIMER_PAIR_PENALTY_1=0.1489
PRIMER_LEFT_1_PENALTY=0.033137
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=TTCTGGAAACCTACCTTCGTGT
PRIMER_RIGHT_1_SEQUENCE=AAGGCCTATGAATGGCTACAGA
PRIMER_LEFT_1=571,22
PRIMER_RIGHT_1=691,22
PRIMER_LEFT_1_TM=60.033
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=121
PRIMER_PAIR_PENALTY_2=0.1497
PRIMER_LEFT_2_PENALTY=0.033905
PRIMER_RIGHT_2_PENALTY=0.115769
PRIMER_LEFT_2_SEQUENCE=TATTATCCCAGTCCAATGGAGG
PRIMER_RIGHT_2_SEQUENCE=AAGGCCTATGAATGGCTACAGA
PRIMER_LEFT_2=477,22
PRIMER_RIGHT_2=691,22
PRIMER_LEFT_2_TM=60.034
PRIMER_RIGHT_2_TM=60.116
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=0.2065
PRIMER_LEFT_3_PENALTY=0.033137
PRIMER_RIGHT_3_PENALTY=0.173354
PRIMER_LEFT_3_SEQUENCE=GTTCTGGAAACCTACCTTCGTG
PRIMER_RIGHT_3_SEQUENCE=CTATCCTGGACCTCCAATTGAC
PRIMER_LEFT_3=570,22
PRIMER_RIGHT_3=724,22
PRIMER_LEFT_3_TM=60.033
PRIMER_RIGHT_3_TM=59.827
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=0.2065
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.173354
PRIMER_LEFT_4_SEQUENCE=TTCTGGAAACCTACCTTCGTGT
PRIMER_RIGHT_4_SEQUENCE=CTATCCTGGACCTCCAATTGAC
PRIMER_LEFT_4=571,22
PRIMER_RIGHT_4=724,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=59.827
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_05167 Ps_contig_mira-and-tgicl-ass_franssen_36694
SEQUENCE=AANTAATAGTTCCAATTTAAAACAAGTCATTAATGGAAACCAAATGTTGGTTAACATTAGAATAGTACTAATCATGCATGATAATACATGATTCAACAAAATAATAATAATACTAAGGTCTCTAACATTCAACTTCATGAAATCTTATAACAATAATAATATCTCAAATGAATTGCATCATAATATTAAGCAAATATGCTCATAGGTCCTGTCTTATTTGATGCATCTAATCCACATTCACCATTCCTAGCTCTTGTGATGCAAACCCTTCGAGTATCCTTGCTAGCTGAATCCATCAGAATTGTTAATCTTTTTGCTGTTTTCATGAAGTCACTGAAGGGTTCTTCACCGGTTTGTTTAACAGTACCTGTCGCGTCACGATAGAGTACGCGACTCAACATCTCGGATCTTTCTATACCGAACATCTTAGCCAGTTTTCTGTATAGTTCTTCGTATGAACCAATACATGACAGATCTAAGGTTCTTCCTACATCTTCTGACTCCAAGAAAACCTTACAATGACCGGTATCTAATCCAAGCTGTTGCCAACAAAACTCGGTAGTACTAGTTGTGGTAATAGAAGATTTTCCCGGTGAAAACTGTTCGGAAATTGAAGATTGAGAAGTATCACTTAAAAACCATTTCTCTTTGTCTTTATCACTAGAAGCTTTTCTAGAGATTTGTTGCTCGGTGAGAATCGGTTGACCGAAAAGCAAAAATTGGTGTCTCTTAACATTACTATCAGAATTTATCTCCAAACTTCTCTTATGAGAGTTTCCTATGGTCAACAAACATGATAAACTTTCTTTGCTCTTATCATTAGAAATCGTCTCGGGGTTATTCGGAAACACACCTAACTGAATTCTATTGTTGTTGATGTTGTTTACGCGAAGATCTGATAAAGATATTCCAATTTGAGCATGCCTGGCTCCCTGAATGCCTGCGGCAGGAGCGTTATAATGATCGGAAGAAGAAGATAGACAACACAACGGATTAAGAGGGTTGCCTGAAAACGTCGGTATTGGAAACTGAACAACATCAAGAGGGAAATCGGGATGTTGAGGAAAACGAGTTT
TARGET=100,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05168 Ps_contig_mira-and-tgicl-ass_franssen_36697
SEQUENCE=TTTCACCCACAAGTGGAACTAATAGGCTTCTAGACTTTCTAATCTTATTATTACTATTATTCCTCTTTTTCCCTTCCAAATATATGGTCATTCTCTCCAAGACTTATTATATGAGCCAGTGTTTGTGATTCTGAATTCTTATGAATATTCTCTCTCTCAACAATCTTCTCAAATCTCACACTCTTTGAAGCCGCTTTTCCAGCTGCTGCAGCTTCAGCTTGAGCCAACTTTGACATGTTCTTGTGAAGCTCAGCGTTCAGTATTGCCAATGCTACCTCCGTTTCACTTCCTCTTTCCTTTAAAATCTTCAGTTCTGTCTTTGTTTTCTCAAGCTCTTCCTCCAGCTTCTTTACGCTGTCAAAAAGTGTTTCACTATTATTACTGATGATGTTTTCTTCTTCTTTAAATGATGATGATTTGATAGGACTAATCAGTGATGGTGATGGCGACAACGGCATTGATCTCCAAGACGATGATTCTCGCTTAATAGTCGCAGAAGGAGAATAACTATAAGATGCGGTAGCAGTATTAGTAGTATCAGTAGTAGTGTCTGAGAATGAGAATGGCTTTGGAAG
TARGET=149,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3012
PRIMER_LEFT_PENALTY=0.298898
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=GAGCCAGTGTTTGTGATTCTGA
PRIMER_RIGHT_SEQUENCE=TGGAGGAAGAGCTTGAGAAAAC
PRIMER_LEFT=112,22
PRIMER_RIGHT=342,22
PRIMER_LEFT_TM=60.299
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.3276
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.219884
PRIMER_LEFT_1_SEQUENCE=CCCACAAGTGGAACTAATAGGC
PRIMER_RIGHT_1_SEQUENCE=AGAACATGTCAAAGTTGGCTCA
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=240,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=59.780
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.4222
PRIMER_LEFT_2_PENALTY=0.298898
PRIMER_RIGHT_2_PENALTY=0.123278
PRIMER_LEFT_2_SEQUENCE=GAGCCAGTGTTTGTGATTCTGA
PRIMER_RIGHT_2_SEQUENCE=CTGGAGGAAGAGCTTGAGAAAA
PRIMER_LEFT_2=112,22
PRIMER_RIGHT_2=343,22
PRIMER_LEFT_2_TM=60.299
PRIMER_RIGHT_2_TM=60.123
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.4496
PRIMER_LEFT_3_PENALTY=0.107763
PRIMER_RIGHT_3_PENALTY=0.341875
PRIMER_LEFT_3_SEQUENCE=CCCACAAGTGGAACTAATAGGC
PRIMER_RIGHT_3_SEQUENCE=GAAGTGAAACGGAGGTAGCATT
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=288,22
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=59.658
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.5188
PRIMER_LEFT_4_PENALTY=0.298898
PRIMER_RIGHT_4_PENALTY=0.219884
PRIMER_LEFT_4_SEQUENCE=GAGCCAGTGTTTGTGATTCTGA
PRIMER_RIGHT_4_SEQUENCE=AGAACATGTCAAAGTTGGCTCA
PRIMER_LEFT_4=112,22
PRIMER_RIGHT_4=240,22
PRIMER_LEFT_4_TM=60.299
PRIMER_RIGHT_4_TM=59.780
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=129
=
PRIMER_SEQUENCE_ID=SSR_05169 Ps_contig_mira-and-tgicl-ass_franssen_36698
SEQUENCE=TAGTTGTTCAAGAAGGGGGTTACGGTATTTAAGTTTGTTGTCTACGCCCGGGACGGGTTGGAAGGATATGTTGGCAAAGAAAAAAAATAGAATCTTGATAACTGCAGGTATTTGACGTTAGATGAGGCTGATCGATTGGTAGATTTGGGATTTGAAGATGATATAAGAGAAGTTTTCGATCACTTTAAAGCTCAAAGGCAAACTCTCTTATTTTCTGCCACTATGCCAACCAAAATTCAAAACTTTGCTCGAAGTGCTTTGGTGAAACCTATTATCGTCAATGTGGGGCGTGCGGGGGCTGCGAATCTTGATGTAATTCAAGAGGTAGAGTATGTCAAACAAGAGGCAAAAATAGTTTACCTCCTTGAGTGCCTACAGAAAACGCCACCTCCTGTTCTGATTTTTTTGTGAGAACAAGGCTGATGTCGATGACATTCATGAGTATCTTCTCTTGAAAGGAGTGGAAGGCGTGGCTATTCATGGAGGCAAGGATCAGGAAGAGAGAGAGTATGCCATTTCATCATTTAAGGCTGGAAAGAAAGATGTGTTGGTTGCAACTGATGTTGCATCTAAAGGTTTGGATTTTCCTGATATTCAGCATGTCATCAACTACGACATGCCAGCTGAAATAGAAAATTATGTCCATAGGATTGGACGTACCGGAAGATGTGGTAAAACTGGTATAGCGACAACGTTTATAAACAAGAACCAAAGTGAGACAACATTGCTTGATTTGAAACACCTATTGCAAGAAGCAAAACAAAGGATTCCTCCAGTTTTAGGTGAGCTGAATGATCCAATGGAAGATACTGAAGAAATCACAGGCATAAGTGGTGTCAAGGGATGTGCGTATTGTGGCGGGCTTGGGCATCGTATCCGTGATTGTCCTAAATTAGAACATCAGAAGAGTGTGGCAATTGCAAATAATAGAAAGGATTATTTTGGATCTGGAGGTTACAGGGGGGAAATTTGAAAGGCGTTGGTCCCTTGTTTTACTAATTTTTATTTTAATACTAATGGCTTATGTCTGCAGATTTTAGATTGTATACCATGAAATCTAATGATCTTCCTTCCTAGACATTTGTACTGATGTTTATGGTATGTACCAATTTTCTCTGAACGCAACTGGGCTATGTATAATATAGATTTGCTAACCTTGAAGTTTTTGCCATGAAGTTAAGTGAATTTCATCACCTGTACTAGTGCCTTGGTAAGAAATACGATTGAATTGTATTGTGTTATAACTCGTTTAGAACTGAGTTGTAGATTAATTTTCTATCTGGATAATTCATTCATTCAGGTGATTTGC
TARGET=499,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1081
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.073989
PRIMER_LEFT_SEQUENCE=ACCTATTATCGTCAATGTGGGG
PRIMER_RIGHT_SEQUENCE=CAGTTGCAACCAACACATCTTT
PRIMER_LEFT=266,22
PRIMER_RIGHT=559,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=294
PRIMER_PAIR_PENALTY_1=0.2162
PRIMER_LEFT_1_PENALTY=0.142170
PRIMER_RIGHT_1_PENALTY=0.073989
PRIMER_LEFT_1_SEQUENCE=TTCTCTTGAAAGGAGTGGAAGG
PRIMER_RIGHT_1_SEQUENCE=CAGTTGCAACCAACACATCTTT
PRIMER_LEFT_1=446,22
PRIMER_RIGHT_1=559,22
PRIMER_LEFT_1_TM=59.858
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=114
PRIMER_PAIR_PENALTY_2=0.3543
PRIMER_LEFT_2_PENALTY=0.142170
PRIMER_RIGHT_2_PENALTY=0.212099
PRIMER_LEFT_2_SEQUENCE=TTCTCTTGAAAGGAGTGGAAGG
PRIMER_RIGHT_2_SEQUENCE=TGTCGTAGTTGATGACATGCTG
PRIMER_LEFT_2=446,22
PRIMER_RIGHT_2=616,22
PRIMER_LEFT_2_TM=59.858
PRIMER_RIGHT_2_TM=59.788
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.3577
PRIMER_LEFT_3_PENALTY=0.283676
PRIMER_RIGHT_3_PENALTY=0.073989
PRIMER_LEFT_3_SEQUENCE=TTTTGTGAGAACAAGGCTGATG
PRIMER_RIGHT_3_SEQUENCE=CAGTTGCAACCAACACATCTTT
PRIMER_LEFT_3=403,22
PRIMER_RIGHT_3=559,22
PRIMER_LEFT_3_TM=60.284
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.3729
PRIMER_LEFT_4_PENALTY=0.298898
PRIMER_RIGHT_4_PENALTY=0.073989
PRIMER_LEFT_4_SEQUENCE=TTGTGAGAACAAGGCTGATGTC
PRIMER_RIGHT_4_SEQUENCE=CAGTTGCAACCAACACATCTTT
PRIMER_LEFT_4=405,22
PRIMER_RIGHT_4=559,22
PRIMER_LEFT_4_TM=60.299
PRIMER_RIGHT_4_TM=60.074
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_05170 Ps_contig_mira-and-tgicl-ass_franssen_36698
SEQUENCE=TAGTTGTTCAAGAAGGGGGTTACGGTATTTAAGTTTGTTGTCTACGCCCGGGACGGGTTGGAAGGATATGTTGGCAAAGAAAAAAAATAGAATCTTGATAACTGCAGGTATTTGACGTTAGATGAGGCTGATCGATTGGTAGATTTGGGATTTGAAGATGATATAAGAGAAGTTTTCGATCACTTTAAAGCTCAAAGGCAAACTCTCTTATTTTCTGCCACTATGCCAACCAAAATTCAAAACTTTGCTCGAAGTGCTTTGGTGAAACCTATTATCGTCAATGTGGGGCGTGCGGGGGCTGCGAATCTTGATGTAATTCAAGAGGTAGAGTATGTCAAACAAGAGGCAAAAATAGTTTACCTCCTTGAGTGCCTACAGAAAACGCCACCTCCTGTTCTGATTTTTTTGTGAGAACAAGGCTGATGTCGATGACATTCATGAGTATCTTCTCTTGAAAGGAGTGGAAGGCGTGGCTATTCATGGAGGCAAGGATCAGGAAGAGAGAGAGTATGCCATTTCATCATTTAAGGCTGGAAAGAAAGATGTGTTGGTTGCAACTGATGTTGCATCTAAAGGTTTGGATTTTCCTGATATTCAGCATGTCATCAACTACGACATGCCAGCTGAAATAGAAAATTATGTCCATAGGATTGGACGTACCGGAAGATGTGGTAAAACTGGTATAGCGACAACGTTTATAAACAAGAACCAAAGTGAGACAACATTGCTTGATTTGAAACACCTATTGCAAGAAGCAAAACAAAGGATTCCTCCAGTTTTAGGTGAGCTGAATGATCCAATGGAAGATACTGAAGAAATCACAGGCATAAGTGGTGTCAAGGGATGTGCGTATTGTGGCGGGCTTGGGCATCGTATCCGTGATTGTCCTAAATTAGAACATCAGAAGAGTGTGGCAATTGCAAATAATAGAAAGGATTATTTTGGATCTGGAGGTTACAGGGGGGAAATTTGAAAGGCGTTGGTCCCTTGTTTTACTAATTTTTATTTTAATACTAATGGCTTATGTCTGCAGATTTTAGATTGTATACCATGAAATCTAATGATCTTCCTTCCTAGACATTTGTACTGATGTTTATGGTATGTACCAATTTTCTCTGAACGCAACTGGGCTATGTATAATATAGATTTGCTAACCTTGAAGTTTTTGCCATGAAGTTAAGTGAATTTCATCACCTGTACTAGTGCCTTGGTAAGAAATACGATTGAATTGTATTGTGTTATAACTCGTTTAGAACTGAGTTGTAGATTAATTTTCTATCTGGATAATTCATTCATTCAGGTGATTTGC
TARGET=1287,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05171 Ps_contig_mira-and-tgicl-ass_franssen_36701
SEQUENCE=GGATACGGTGGAGGAGGAGGTGGTTATRGTGGGGGAGGAAGGTGGTGATGGTAATGTTGATGTTGTGAATTTGGGGACAATAGCGTTGGCTATGGGTTTTGGTTCGAAGTTGTTTGAGTTGGCTTCTGCTCCTGATTGTGTTCAGGGGAATAGAAAGGTTTTGTTTGAACTGCATCGCGAGTTTTTGAAGCTGGAGAAGGATGCTGTTATTTCAGGGTTTGAGTTTTCCATTCCAGATTCTGTTGCAAGGAATGAGGAGGAAGTTCCTGTTGTTGAGGTTGATGCTGATGTTGTTCTTAATGGTAAGGCGCTGAAGAAATGCAAGAAGGATAAGGAAGAATCTGTTGACAAAGTGAAGAAGGAAAAGAAGAGTAAGAAGAAGAAGAAGAAAGATAAGGATTCTGCCGAGAGCGGGGATAAGAATGTTCCTACTGAAAATGGTGGAAAATTGATTGTTGAAGAGGTTGACAACGAGTTGGTTTTGACCGAGACTGTGATTTCAAATCTCCAAAAGCAGTTTGAGAAGGTTGCAGCGGAAGCAGGTTTGGATGACGGTGATGCAAGTTTATGCGTTACACCGAGAGCTACTGAAACTGTATCTAAGAAGAGAAAAAGATCTAAGAGTTCTAAAGGAAAGATATCTCAGGATCTTGAATTGAATGGTGGAGATGCTGAAGACTCTGCAGTAGGTAAGAGTGTGGAGAAAAGTTTGAAGAGGGTAAGATTTTCCATGAAAAATAACTTGGTATGGAAGCCACACAGTCCTTTACCTCCTCAGAGTTTGAGAATTCCTCCCTCTGTTACACCAAGAGGAAGTGCGCTCAAAAAGGGCGTTCCCCCAGGTCCCATCAGGGAGATGTCTTCCCAGACCAAAAAGGTGAAACTGAAGAAGGTCCGGAGGACAATAATTGGTACACCATCTGTCAAGCGCTTGAGGAAATTGAGAGCTCTTTCTGTGTGATTTTCTTGATGTTGTTAAGGGATGAATTTTCCATGATTTATTTTGTTGTACTTTTTATTTGTAACTGTTAAGAAATTTTGAAACGTCACTATGATGGTTGATTCAAGTTCGTTTTAANAGAAAAAACG
TARGET=374,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05172 Ps_contig_mira-and-tgicl-ass_franssen_36703
SEQUENCE=GGTACAAACAAAAAATAAATTCYATATCAATATTAATGGTAAAACATTTCATTCTAGTATTAAGTTGAATATTAGTACATTTATAGGATTCTCAACACTCATGTAGAAGTCACTAAATAAACTACATAATAACAAAATGCAGAAAGTTCCATCAAATGTAATGCTTGGAGAAGATTCATTACAGGGATACATATTTTTGCTGTCTTCAAACAGGATCAAGAGGAATTTTCTTCCTTGTCAGACTCATCAGGTAAACCTCTCACCGTGATGGTAATATGACAAGTGCGCTTTAGAATTTGGTTAGCCCGCCCCTGAGCTCGAGCTCTTACTCTTTTCAAAGTCTTGCCTTCATTAACCTCTGCTTTACTAATAAACAAACTTGATTTGCTCAGACCCAAGTTATGACTTGCATTTGCTCCAGCAGAAAACACAATCTTAATAATGGCTTCACAGGCACGATAAGGCATGAGTTCCAATACCATGAGTGATGCCTCGTACGGACGCCCGCGAATCTGATCGACTACTCTTCGAGCTTTGTCAGCGGACATTCGAATGTGTCTCCCTACAGCATAGGTTTCTGCGTATGGTTGGTTGGTCTCCTGAACTTCGAGCTGGGTAGCGTTAAAGCGGCAAACGAAAGAGTTTTTGTTGTTGGGGGTAACCGTTTTGAGGGAAATGTCATTGAAAGAGGAGGAGGAACATCGAATTCGGGCGGAGGAGGGTATCGGGAATTTGACTAGGTTTGGACGGAATTGGGATTGAAATGGTTGGGGTCGAAGCAGGTTATCGCGAACAGGAGGAACGTTAATGGCTAAAGAGAGAGCCATTCCGATGATGATGAGTGAATGAAAAACCTAAGATAAGACTGGATCGATAACAAATGAAAACATATCAACTTATCCCTCYTCTTATTTTTTCTGTTCTTTTATTTACTTTTAAATTAAGTAAACTTATTTGAAAATAATTTTTTTTCTGTTCTTTTATTTACTTTTCAATTAATTCTTTTTATATTATTTAACAACCCAAAACAAAAAGTATTAAGAATGAAATTTATTTTAAATTTAATTTTTAAATACTCTTACTAAAACTTTTTCTTAGTTTAGTTATTTTTTAGACGATATTTCAAATAATGTTTTATATTTTATAATTTATTAATTTTTTTAAAATTTTTATCTTTACATGTTTTATTAAAATTCACTTTGTCATTTATTATTTATTTTAATTTAAAATAAAACAATAATGTATTAAATATTTTTTATGTTATTTTATATTTATCAATTAATAAAATATTAAATTTATCAAACACTTCAACTAATTTATCAATTATTAGTCATCATCTATAACTAAAATATGAACTCGTTTGTTTTGATTTAAAAAAAAAATTATAGGTATAATATGATTT
TARGET=585,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05173 Ps_contig_mira-and-tgicl-ass_franssen_36710
SEQUENCE=TCGGGGGACGGCTGTTGTATAAACAGAGTGCCATTCCATCACARCAGAACACATCGCATCTTCCCATAGACACTATCTTCTTCTTCTTCTTCTTCACCTGCACTCTGCAATGTGCAATGCTCACACCATTATCATCAGCATCACTTTTCACCAACAATAAACCCTTTCTTTTCACATTTCTTCTTCCTTTTATTTATCCCAATTAAATCCCCACTATCTCTTTCAACATTCAATCAATTCCCAATTATTAGGTTTTTTTTATATAATTTAAATGTGGTGGAACTCCAATCACCAATGGAACCGCCTCAACCATCCAAATCAATTGATTCCTCCACCAAAGACCCACAATTGGATTCTTCCCCTGTTCTCCACCATGATCACAATGATTCTCAACTCTCAGATCTCAATTTCTACGTGACCCACCTTGATGAGGAGCTTCAGAACAAGTTGGATTTGAAAGATGAGGATCAAATTCATGAAAAAGGTTCAAACTTTGAAGCTGCTTCTGTAGAATCTGATAAGGGCCAAGAGTGTCCTAATGATGATGGTTGGAATGGTGATGATGATGACGGGTGGAATGGGGATGATGAGGTGAATGACGTGGGTTGAGAATGTAGAA
TARGET=76,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05174 Ps_contig_mira-and-tgicl-ass_franssen_36710
SEQUENCE=TCGGGGGACGGCTGTTGTATAAACAGAGTGCCATTCCATCACARCAGAACACATCGCATCTTCCCATAGACACTATCTTCTTCTTCTTCTTCTTCACCTGCACTCTGCAATGTGCAATGCTCACACCATTATCATCAGCATCACTTTTCACCAACAATAAACCCTTTCTTTTCACATTTCTTCTTCCTTTTATTTATCCCAATTAAATCCCCACTATCTCTTTCAACATTCAATCAATTCCCAATTATTAGGTTTTTTTTATATAATTTAAATGTGGTGGAACTCCAATCACCAATGGAACCGCCTCAACCATCCAAATCAATTGATTCCTCCACCAAAGACCCACAATTGGATTCTTCCCCTGTTCTCCACCATGATCACAATGATTCTCAACTCTCAGATCTCAATTTCTACGTGACCCACCTTGATGAGGAGCTTCAGAACAAGTTGGATTTGAAAGATGAGGATCAAATTCATGAAAAAGGTTCAAACTTTGAAGCTGCTTCTGTAGAATCTGATAAGGGCCAAGAGTGTCCTAATGATGATGGTTGGAATGGTGATGATGATGACGGGTGGAATGGGGATGATGAGGTGAATGACGTGGGTTGAGAATGTAGAA
TARGET=558,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05175 Ps_contig_mira-and-tgicl-ass_franssen_36727
SEQUENCE=CGNGGGGATCCACTAATGAGATAAACCTAGCCGCTAGACGAAACCTAAATCTCTTGGAGATTCATATAATTGAAATGGAACAATCAACTACACCAAAAGTACCGACTATACCCGAAATTAAAATCAAATCTAATCAGAAAAAGAAGATTCCTTCTCCTAAGGAGTTGATATCTTATTATGAATCTCAAGGGATGGATTCACATGAAGCGTCTTTGAAGGTAATTGAGGATTTGCAAAAAGCTTTATTTGGAGTGATTTCATCTGGAAGAGGTAAAAACGATAGGGTTTCGAATGAATCTTCTAGAATGATGGATTTGGTAAACAATAGACTTGCGGTACTTGACATGAAGTTGGATACCAAGCCTGGTTATGTTGAGACGTTTGCAATTGGTGTTGTTTCTGGTGCAGCTCTGAATGGATTTGGGGCTATATTGCCTCGTATTATTTCACCTATTGCTCAGATTTGGAATTCTGTTACTACGGCCACTAAATCTGCACCTCAATGACATTTTTGATTCATTTTTAGTTTTGTATGTATGTACACTATTATTCAATTTAATCTATTCGTAAATTTTGTATTTTTGTTTAAATGAATTATTAAAGTAAATATTTGACNGAACTT
TARGET=530,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05176 Ps_contig_mira-and-tgicl-ass_franssen_36733
SEQUENCE=TCGGGGTATTNNAAAATATTTGTGCGCAACTGGTTTTACTTTCTCCCGCAGAAAAGAACCAAACCAACCAACCAGCTCAACGCCAGAGTGGAAGAAGATGGAATCGCCATCAACGACGGAAGATACAAAGAAACTAGCAGTTAGGTCATCGTTCGTCGAGTCAATTCGCGGCTGCGGCCTCTCCGGCATCCGTATTGACAAAGAAGACTTGAAGAAGCAGCTCACCATGCCTCAGTATCTTCGCTTCGCAATGCGTGATTCCATTCGTCTTCAGGATCCCGCCGCCGGAGAGCCTCGATACCGGAATCGTGCTCAAGGAGAGGATGCTTCTGTTCCTTCTTCTCCTATGGTAGTTTTCATTAACTCACGTAGCGGTGGACGTCATGGTCCTGCTCTTAAAGCCAGGCTCCAGCAACTCATGAGCGAAGAACAGGTTTTTGATTTATCAGATGTGAAGCCTCATGAATTTGTAAAGTATGGATTGGCCTGTTTGGAGATGTTGGCTGCTCTTGGAGATTCTTGTGACTAAAGAAACTCGTACAAAGACTCAGGGTTATGGTTGCGTGGAGGTG
TARGET=62,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0321
PRIMER_LEFT_PENALTY=0.028536
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=CAACTGGTTTTACTTTCTCCCG
PRIMER_RIGHT_SEQUENCE=TTTGTATCTTCCGTCGTTGATG
PRIMER_LEFT=26,22
PRIMER_RIGHT=128,22
PRIMER_LEFT_TM=60.029
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=103
PRIMER_PAIR_PENALTY_1=0.1189
PRIMER_LEFT_1_PENALTY=0.028536
PRIMER_RIGHT_1_PENALTY=0.090376
PRIMER_LEFT_1_SEQUENCE=CAACTGGTTTTACTTTCTCCCG
PRIMER_RIGHT_1_SEQUENCE=GATCCTGAAGACGAATGGAATC
PRIMER_LEFT_1=26,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=60.029
PRIMER_RIGHT_1_TM=59.910
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.1658
PRIMER_LEFT_2_PENALTY=0.162217
PRIMER_RIGHT_2_PENALTY=0.003589
PRIMER_LEFT_2_SEQUENCE=CGCAACTGGTTTTACTTTCTCC
PRIMER_RIGHT_2_SEQUENCE=TTTGTATCTTCCGTCGTTGATG
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=128,22
PRIMER_LEFT_2_TM=60.162
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=0.2243
PRIMER_LEFT_3_PENALTY=0.028536
PRIMER_RIGHT_3_PENALTY=0.195770
PRIMER_LEFT_3_SEQUENCE=CAACTGGTTTTACTTTCTCCCG
PRIMER_RIGHT_3_SEQUENCE=GGTGAGCTGCTTCTTCAAGTCT
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=225,22
PRIMER_LEFT_3_TM=60.029
PRIMER_RIGHT_3_TM=60.196
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=0.2526
PRIMER_LEFT_4_PENALTY=0.162217
PRIMER_RIGHT_4_PENALTY=0.090376
PRIMER_LEFT_4_SEQUENCE=CGCAACTGGTTTTACTTTCTCC
PRIMER_RIGHT_4_SEQUENCE=GATCCTGAAGACGAATGGAATC
PRIMER_LEFT_4=24,22
PRIMER_RIGHT_4=277,22
PRIMER_LEFT_4_TM=60.162
PRIMER_RIGHT_4_TM=59.910
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_05177 Ps_contig_mira-and-tgicl-ass_franssen_36740
SEQUENCE=GTCGGGRAAAAGGAAAAAAGACGTAGCAAATCTGAATCGAATAAACCAAATCACAATTTTATTTTCTTCAATAAAATAACACTAGCGTTTCTTTTCCTCTCTTTTCTCCATTGCCTCCTCCTTCTTCTTCTTCTTCCCTTACAGGTTATTGTAATTATAATAACTATAAAAAGTGTAATCTGAGAAGGGAAGGTTTTTTTTTGGCAATGGAGGAACCTAGCCAAGTGAAACGTGCTATCATTGATTCATCTGCTGGAGCAATTTCTGGGGGAATTTCTCGCACTGTAACATCACCACTTGATGTTATTAAGATTAGATTTCAGGTTCAGCTTGAACCAACATCTTCATGGGCTTTACTGCGCAAAGATTTGGTCTTAACCGCGCCGTCGAAATATACCGGTATGCTTCAAGCTAGTAAAGATATTCTTAGAGAAGAAGGCTTTAAGGGTTTTTGGCGAGGGAATGTACCGGCTTTGCTCATGGTTATGCCGTATACAGCAATTCAGTTTACTAGTTTTACACAAGTTAAAGACTCTTGCATCTGGTTCTTCCAAGACAGAGAATCACACTAATTTGAGCCCTTATCTATCCTATGTCAGTGGTGCCCTAGCTGGGTGTGCAGCCACGGTTGGTTCATATCCTTTTGATCTTCTCCGAACCATATTAGCGTCTCAAGGCGAGCCAAAGGTGTACCCTAACATGAGGTCAGCATTTATGGACATCATCCAGACTCGTGGATTTCAAGGCATGTATGCTGGTTTGTCTCCAACTTTAGTTGAGATTGTACCTTATGCAGGCCTACAATTTGGAACATATGATACTTTTAAGCGTTGGACCTCGGCTTGGAACCATAATAGATATTCCCACACCACGGGAGATGATAGTATTTCTAGCTTTCAGCTTTTCCTTTGTGGGTTGGCGGCTGGGACATGTGCGAAACTTGTCTGTCATCCTCTTGATGTGGTCAAGAAAAGATTTCAGATCGAAGGGCTTCAAAGGCATCCAAGATACGGAGCTCGAGTTGAGCATCGAGCATACAGTAATRTGTACGATAGCCGTGCATAGGATTAGTTTCGGTCAGGTAGGGGGTTTGGGGCGTTCGG
TARGET=121,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05178 Ps_contig_mira-and-tgicl-ass_franssen_36741
SEQUENCE=TTTGAAGCTGTCGTTAACAGGTTGAGGAGTTCCGTGAGGTCCGAAAGGAAAGGAGAGTGATTGTAGTGTTGAAGCAGGGTATTGGGGAGGTCCGAATGGTTCAGAGTAGAGGGAAGGTTTGGTGATTTGTGAAGTGAGGGTTTGTTGAATCCTCGTGAAGATGATGGAGAGGGTGAAGAAGAAGAAGAAGCGGAAAATGATTTCATTCTGGTTTTTATCGTGAAGGGGTTGAAGAAGTTGGTTAGGTCCGAACTTTCTCTACGATCGTCAGACAACATACTCCTCCTAGCTGCAACTAAGCCCGAGATTGTAGATTAGTCCCCGCCTGCTGAAATCTTTGAAGCATTATCCTGGTTCTTCCCAGCTTTCGCCCAAGCAGCACGGTGAGTAAGTGGACCTGAATGTAAGGCTCTTTTATGTTGATTTGCTTGGGGATCAACAGTTTCTTCTGCAACTTATGATGGTCTTGCAGCTTGGAAATGAATGCCCTGCTTATGCTTGAAAGTGCTTATGATACGATGTTGAATTGATGTAAATCCATGGCCTTGTTAGTTGTTTTAGACTGTTTTGTGTTATGTAATTGAACAAACGGTTGAATCTTGAATTTGGTTGCATTAGACTAAAAAAACTGTTATGTACTGCATTTGCTATTTTGGACTTCAATGTTATGAGCTGCATCAAATTTAAAAAACCGT
TARGET=175,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0281
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.019805
PRIMER_LEFT_SEQUENCE=TTTGGTGATTTGTGAAGTGAGG
PRIMER_RIGHT_SEQUENCE=CGGGGACTAATCTACAATCTCG
PRIMER_LEFT=117,22
PRIMER_RIGHT=323,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.0281
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.019805
PRIMER_LEFT_1_SEQUENCE=TGATTTGTGAAGTGAGGGTTTG
PRIMER_RIGHT_1_SEQUENCE=CGGGGACTAATCTACAATCTCG
PRIMER_LEFT_1=122,22
PRIMER_RIGHT_1=323,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=59.980
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.0649
PRIMER_LEFT_2_PENALTY=0.045112
PRIMER_RIGHT_2_PENALTY=0.019805
PRIMER_LEFT_2_SEQUENCE=TTTGTGAAGTGAGGGTTTGTTG
PRIMER_RIGHT_2_SEQUENCE=CGGGGACTAATCTACAATCTCG
PRIMER_LEFT_2=125,22
PRIMER_RIGHT_2=323,22
PRIMER_LEFT_2_TM=60.045
PRIMER_RIGHT_2_TM=59.980
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=0.1386
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=TGATTTGTGAAGTGAGGGTTTG
PRIMER_RIGHT_3_SEQUENCE=AAGAGCCTTACATTCAGGTCCA
PRIMER_LEFT_3=122,22
PRIMER_RIGHT_3=413,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=292
PRIMER_PAIR_PENALTY_4=0.1386
PRIMER_LEFT_4_PENALTY=0.008257
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=TTTGGTGATTTGTGAAGTGAGG
PRIMER_RIGHT_4_SEQUENCE=AAGAGCCTTACATTCAGGTCCA
PRIMER_LEFT_4=117,22
PRIMER_RIGHT_4=413,22
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_05179 Ps_contig_mira-and-tgicl-ass_franssen_36746
SEQUENCE=AGAGAGAAGACTTACTAGTTTCAAAGCAGCGACAGAAATAAGTATTAAACCTCCTTCCTCATGCTTCTTCTTCTTCATTCCCAAGTCAATCTTCTCCTTCTCTGTAATTCTGATTTGACTTCTTTGTTGCGTGAGAGTTGCGAATCGTGATTGAAATGCTGCAGATCGAAGGTTGCGGATTGGTAAACTCAAGTTGCAGAAGGTGAATTCAAGTTGTAGAAGGTCGAGCTCCAAGTTGCAGAAAGTGAACTCAAGTTGCAGAATGAAGTTTCAGCTGCATAATCGAGTGTCTGAGTTTACAAAGCAAAATTTCTCGGTTGCAGCATGAAGTATCAAGATGCAGAGTGGAGAAATTTAGATGTCGAATGAGAAGAACTCTGATGCTGAATTGAAAAGCTCTGATGCAAAATGAAGAATTTGATGCGGAATGAAAAATTCCCCAGGTTGAAGATAGGTTGTATGGTTGATGATTGTATGGAAAATGTTTTCCTATTGTACTTTGTTGCTATATGGTTGCTTAAAAAGTCAGGTTGTCTTTGCATTGAATCAAGACCTTAAAACATGAGCATCATTAGCATCTCATGGCTAAATGCAAACTAAGACTTCTTGTGATGGAAGCAAGCTTGGATGGAAGATTTAATGAGGTGATGCAACATGAAGACTCAAGATCCATGAAATTTAGGATTTAGGTTTAGAGAAATAAAGGGTTGAATTTGGTCAAAGTTGATCAAAAAGTCAACTATTGACCAAAGCCTAACTTTTGGTCAAAGTGTCAATTTTTAGTTAATTTTTTAGTATGAATACTTGGTTAAACGGTAATTTGTTATAGAATGAATGAATAACTTTAAAAT
TARGET=64,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2160
PRIMER_LEFT_PENALTY=0.171564
PRIMER_RIGHT_PENALTY=0.044457
PRIMER_LEFT_SEQUENCE=CTAGTTTCAAAGCAGCGACAGA
PRIMER_RIGHT_SEQUENCE=CTCAGACACTCGATTATGCAGC
PRIMER_LEFT=14,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=59.828
PRIMER_RIGHT_TM=60.044
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.2308
PRIMER_LEFT_1_PENALTY=0.186309
PRIMER_RIGHT_1_PENALTY=0.044457
PRIMER_LEFT_1_SEQUENCE=TAGTTTCAAAGCAGCGACAGAA
PRIMER_RIGHT_1_SEQUENCE=CTCAGACACTCGATTATGCAGC
PRIMER_LEFT_1=15,22
PRIMER_RIGHT_1=294,22
PRIMER_LEFT_1_TM=60.186
PRIMER_RIGHT_1_TM=60.044
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.2519
PRIMER_LEFT_2_PENALTY=0.171564
PRIMER_RIGHT_2_PENALTY=0.080323
PRIMER_LEFT_2_SEQUENCE=CTAGTTTCAAAGCAGCGACAGA
PRIMER_RIGHT_2_SEQUENCE=CGCAACAAAGAAGTCAAATCAG
PRIMER_LEFT_2=14,22
PRIMER_RIGHT_2=130,22
PRIMER_LEFT_2_TM=59.828
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=0.2600
PRIMER_LEFT_3_PENALTY=0.171564
PRIMER_RIGHT_3_PENALTY=0.088479
PRIMER_LEFT_3_SEQUENCE=CTAGTTTCAAAGCAGCGACAGA
PRIMER_RIGHT_3_SEQUENCE=CAACTCTCACGCAACAAAGAAG
PRIMER_LEFT_3=14,22
PRIMER_RIGHT_3=139,22
PRIMER_LEFT_3_TM=59.828
PRIMER_RIGHT_3_TM=60.088
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.2607
PRIMER_LEFT_4_PENALTY=0.171564
PRIMER_RIGHT_4_PENALTY=0.089133
PRIMER_LEFT_4_SEQUENCE=CTAGTTTCAAAGCAGCGACAGA
PRIMER_RIGHT_4_SEQUENCE=CACTCGATTATGCAGCTGAAAC
PRIMER_LEFT_4=14,22
PRIMER_RIGHT_4=288,22
PRIMER_LEFT_4_TM=59.828
PRIMER_RIGHT_4_TM=59.911
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_05180 Ps_contig_mira-and-tgicl-ass_franssen_36746
SEQUENCE=AGAGAGAAGACTTACTAGTTTCAAAGCAGCGACAGAAATAAGTATTAAACCTCCTTCCTCATGCTTCTTCTTCTTCATTCCCAAGTCAATCTTCTCCTTCTCTGTAATTCTGATTTGACTTCTTTGTTGCGTGAGAGTTGCGAATCGTGATTGAAATGCTGCAGATCGAAGGTTGCGGATTGGTAAACTCAAGTTGCAGAAGGTGAATTCAAGTTGTAGAAGGTCGAGCTCCAAGTTGCAGAAAGTGAACTCAAGTTGCAGAATGAAGTTTCAGCTGCATAATCGAGTGTCTGAGTTTACAAAGCAAAATTTCTCGGTTGCAGCATGAAGTATCAAGATGCAGAGTGGAGAAATTTAGATGTCGAATGAGAAGAACTCTGATGCTGAATTGAAAAGCTCTGATGCAAAATGAAGAATTTGATGCGGAATGAAAAATTCCCCAGGTTGAAGATAGGTTGTATGGTTGATGATTGTATGGAAAATGTTTTCCTATTGTACTTTGTTGCTATATGGTTGCTTAAAAAGTCAGGTTGTCTTTGCATTGAATCAAGACCTTAAAACATGAGCATCATTAGCATCTCATGGCTAAATGCAAACTAAGACTTCTTGTGATGGAAGCAAGCTTGGATGGAAGATTTAATGAGGTGATGCAACATGAAGACTCAAGATCCATGAAATTTAGGATTTAGGTTTAGAGAAATAAAGGGTTGAATTTGGTCAAAGTTGATCAAAAAGTCAACTATTGACCAAAGCCTAACTTTTGGTCAAAGTGTCAATTTTTAGTTAATTTTTTAGTATGAATACTTGGTTAAACGGTAATTTGTTATAGAATGAATGAATAACTTTAAAAT
TARGET=829,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05181 Ps_contig_mira-and-tgicl-ass_franssen_36747
SEQUENCE=AAATGCAATACTGCAGCAAGCTGCATTTACCATATAATGAACCACGAGAAAAACGTGCAGAAGTTCCACAATTCATTAAAAATCTAACACCAAGCAATTTTACATTTAAGACTCATGGCATCATGTTAGCCAACACCATCATACTCCAACTAGATTAAAAGCTTATTTACTGCATCTCAAACCTTGTTGACACAACCGTTTCGCATTTCAGGAGCACAAATTACTCCTTGGAAATTGTTATCTTAAGCTCACATTAGTGTTAAAAAGTTTTGACTCTGTAACCAAACACAGCAAGTCCGTGAGGCTTTTTAAGCACACATTAGGGTTAAAAGTTGACTTTGTCTTGCTGAAATGTTATTGTATGCTGAATGCCAGGCATGAAGATTATAGAAGCAGCGAAGGCCTTCAATGCTATAACTCTAGGAGATTGACAGGTTTCTTGAACTGATATCTATCGTGAAAGTAGTTTGTATGAGGGCAAGTCTTCAAGACGCAATACTTCTTCTTGAAGAAGTTCTTTCTCTAGCTCACGCCGTGTAGATTTCATCACTGTATTAAAGTCCATGTATGAGGCGCGCATAGAAAGCCATTGGTGATCCATCAGCTTGAAAGCTATGCAGTAGAGAAGATCAAAAGCTGATTCATTTTCTTCAAGAAACTTTAGGAAAGTTGCTCCCACCAGTGTGCGTGGCTTCACAGCTTCAAGATCAAGCATCTGCACCAGCATGAATGTGATGTTAACACCAGCAACAGCAAACGGATATTCCCACGCGGACCGATCTCCTTCCCGCTTCCATAAAAGATCCTGGAACGATTTCGGGAAATTCCTAGCAAAGAACAGAAAATTCTCCAAAGAAATAAAACCACCACCCCTAAAATCTGTTGATGGATCCTTCCCTTGCCAGCCCATTTCCTTCCATTGCTCAGATATCAAGCCACGGAGCTCCTCTTCAGGAAATGCAACATTCCATAAAGCCCTAAGGGCCTCCTGGTGCTCAATTATAGAACTATCATATGGAACATCTATTCGTCTCTGTAGCCTCTGCAAGCATTCCTCCTGGGCTAGAGTTAAATCAAAAAAGCTAGAAGCATCACTATCTCTTCTCTGTACACATACACAAGACAGACTTCTACCAAGCCATGCTGCTGATCCTGCCACAGCCTCCGCAGAATTTGAATTGCAGGTTTCTCCATGCGGTGTTGTTCGTCTAACAGCAACGAATGAAGCACCTCTATTATCCATTTTCTTCTTCAACAATATCTACTACTATAGCATCATCATCATCACCAACCTGTAGTCCATTATTTAACCGTAAAATCTCGCCGTCGAAATCTGAAGACGCCGGAAACCGTAAAATCTCGCCGTCGAAATCTGACGATGCCGGAAACGAACGGAGCGTGAATCGAGATAGAGTGGTGGATGAGAATGAAGAATGGAATAGGAGAAAGCGGGAGAGTTAGGGTTTGATTGAGATCTGAGAGGAATAGAATAATATAAAAAGAGTTTTGGAATGAAAAACGGTTACGGTTAGAGTTGGAGATGAAGAGAAAACTCTCACTCCGCTCTTATGCTTTGCTTCACCTTCCTTACCCCGC
TARGET=1274,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1198
PRIMER_LEFT_PENALTY=0.052366
PRIMER_RIGHT_PENALTY=0.067436
PRIMER_LEFT_SEQUENCE=CTAACAGCAACGAATGAAGCAC
PRIMER_RIGHT_SEQUENCE=CATTCTTCATTCTCATCCACCA
PRIMER_LEFT=1208,22
PRIMER_RIGHT=1435,22
PRIMER_LEFT_TM=59.948
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=0.1263
PRIMER_LEFT_1_PENALTY=0.058853
PRIMER_RIGHT_1_PENALTY=0.067436
PRIMER_LEFT_1_SEQUENCE=CGTCTAACAGCAACGAATGAAG
PRIMER_RIGHT_1_SEQUENCE=CATTCTTCATTCTCATCCACCA
PRIMER_LEFT_1=1205,22
PRIMER_RIGHT_1=1435,22
PRIMER_LEFT_1_TM=59.941
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.1759
PRIMER_LEFT_2_PENALTY=0.052366
PRIMER_RIGHT_2_PENALTY=0.123573
PRIMER_LEFT_2_SEQUENCE=CTAACAGCAACGAATGAAGCAC
PRIMER_RIGHT_2_SEQUENCE=ACTCTATCTCGATTCACGCTCC
PRIMER_LEFT_2=1208,22
PRIMER_RIGHT_2=1414,22
PRIMER_LEFT_2_TM=59.948
PRIMER_RIGHT_2_TM=59.876
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.1824
PRIMER_LEFT_3_PENALTY=0.058853
PRIMER_RIGHT_3_PENALTY=0.123573
PRIMER_LEFT_3_SEQUENCE=CGTCTAACAGCAACGAATGAAG
PRIMER_RIGHT_3_SEQUENCE=ACTCTATCTCGATTCACGCTCC
PRIMER_LEFT_3=1205,22
PRIMER_RIGHT_3=1414,22
PRIMER_LEFT_3_TM=59.941
PRIMER_RIGHT_3_TM=59.876
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=0.2548
PRIMER_LEFT_4_PENALTY=0.187326
PRIMER_RIGHT_4_PENALTY=0.067436
PRIMER_LEFT_4_SEQUENCE=GTTCGTCTAACAGCAACGAATG
PRIMER_RIGHT_4_SEQUENCE=CATTCTTCATTCTCATCCACCA
PRIMER_LEFT_4=1202,22
PRIMER_RIGHT_4=1435,22
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=59.933
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_05182 Ps_contig_mira-and-tgicl-ass_franssen_36748
SEQUENCE=CCTTTCGTCTTCTGACCTCAAACCCTACGCAATTCAGTATACAAGNGTTTGTATCAGCTGAARCAAGCGAGTACTGACGAATAAAACAAAAACAACCGCTGAGAAAGRAGTATCNCCAGCATCACTGTCTGCGTTTATGGCCTTCTTTAGGTGCAATTTCAAAGAGCATTGCTACCTGTCTAACCTATCCTGCCATCAGGTGCAAAGTCATCATTCAAGCTGCAGAAGCGGATGACGAAACTTCCGAAAAAAAGAATTAAATCATCGAAAACTGTGTCCAGCGTTCTCTGCGGAATATGGAAAAGGGAGGGCATTTTGGGCTATTTCAAAGGATTACATGCTCAGATATTGAAAACTGTTTTGAGCTCGGCACTGCTCTTGATGATCAAGGAAAAGATCTCTGCCACTACCTGGGTCCTTATTCTTGCAATTAAAAGGTATCTCTTACTCCCAAAGGGCAGGGTTAAGAACTTGTAAGTTTTACTATAGTTGTTATTGGAGGAAGAAGGTTCTACGCATTTTCGATAATGGATTTGGTTAGAATGCAACGTGTGCATTTTACGGATACGTCCATTTCTTTAGAGCTGAGACAAGGTTCTTTTTCTGGCTTCTCTTTAGTCCAATATCCTTCACTAGTCGATGTAGAACAATGTGTCCAAGAATTCATGCAAACGATAGACCATAGCTAGATTGGAAGTCTTTTAACAATAAATGCATTCGAGTTTTGATTTGTTACATTTCAGACACGAAGTAACGAATGTCGTTACTTAGTTTTTGTTTATAGTTTGGTTTTTTTATATTGTTGTGGARATCCTACATCAAGGAAGGATAATTAAGTTAAGGTTAACTTAAGTGTACTTAAATAAATAAAGTANCTTA
TARGET=860,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05183 Ps_contig_mira-and-tgicl-ass_franssen_36749
SEQUENCE=CTTTTCTGACAAAATATATTAAACAAAAATATTATGTACCACCAGCATTGCTTTCCGGCGAGGAAAAGGAATGCAGATAGATAACAAACTGTAAAATGGTGTTTCATAAATACTATGATGATGATGATATCGCCATTTAGTTACTGTATAAACAAATAAATAAACTGGAAACCTAAAGTCCATCGATAAATCTTCTGAACGGGCATTAATGCCTCTAAAAACAGCAACTAGCCCATTTGATGTAGTTCCACATCACTTGGTCAAATCAACCTCTACCTCGCCCTGCACCTCTGTTTCCACCAGGCCTTCCACCTGGGCCACCCCTTCCTCGGCCTCCTCCAGGCCCCCTAGCACCACCTTCTTCAAAGCCACGTCCAGCTCCTTTTACTTGACGTCCAGGAGCGCCATCCTCCCTACCTCTTCCCCTTCCGCGTCCCACACCAGGTGGTTTGCGATCCGTGCGACTCTTGGTTTCTTCCTGGACTTTGTCAATAACCTCATCAGGAACTCGAAGGTACTTAATGGTATTCCCCCGAATGTAGCATTCAGGCATACGCCAAAATCTATCTCCGTCTTTAGAGGTACAAATGACTTCTCGGAGATGAATGTTCATCCATGTATCACAATTAACCAAATGGCCGTTATAAGTCTCTCCATTTTTGAGTTCAACCAACATGGGGTGCCCTTGAGCAGTTTTGAGAAGGGAGAGTGGCAGCATGACGTGAAACGGTGGTTGATTTGAGGAAAGAGAGCGAAGAGGTTGACGACGACGGTGATGATGTCTGCGAGACTCTCNCTTTGGTTTACACTTT
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2191
PRIMER_LEFT_PENALTY=0.216800
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=CGAGGAAAAGGAATGCAGATAG
PRIMER_RIGHT_SEQUENCE=CGAGGTAGAGGTTGATTTGACC
PRIMER_LEFT=58,22
PRIMER_RIGHT=279,22
PRIMER_LEFT_TM=60.217
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.2445
PRIMER_LEFT_1_PENALTY=0.216800
PRIMER_RIGHT_1_PENALTY=0.027715
PRIMER_LEFT_1_SEQUENCE=CGAGGAAAAGGAATGCAGATAG
PRIMER_RIGHT_1_SEQUENCE=ATCAAATGGGCTAGTTGCTGTT
PRIMER_LEFT_1=58,22
PRIMER_RIGHT_1=240,22
PRIMER_LEFT_1_TM=60.217
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.3543
PRIMER_LEFT_2_PENALTY=0.216800
PRIMER_RIGHT_2_PENALTY=0.137489
PRIMER_LEFT_2_SEQUENCE=CGAGGAAAAGGAATGCAGATAG
PRIMER_RIGHT_2_SEQUENCE=GCGAGGTAGAGGTTGATTTGAC
PRIMER_LEFT_2=58,22
PRIMER_RIGHT_2=280,22
PRIMER_LEFT_2_TM=60.217
PRIMER_RIGHT_2_TM=60.137
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.3805
PRIMER_LEFT_3_PENALTY=0.216800
PRIMER_RIGHT_3_PENALTY=0.163658
PRIMER_LEFT_3_SEQUENCE=CGAGGAAAAGGAATGCAGATAG
PRIMER_RIGHT_3_SEQUENCE=GATTTGACCAAGTGATGTGGAA
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=60.217
PRIMER_RIGHT_3_TM=59.836
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.3814
PRIMER_LEFT_4_PENALTY=0.216800
PRIMER_RIGHT_4_PENALTY=0.164639
PRIMER_LEFT_4_SEQUENCE=CGAGGAAAAGGAATGCAGATAG
PRIMER_RIGHT_4_SEQUENCE=ATCGATGGACTTTAGGTTTCCA
PRIMER_LEFT_4=58,22
PRIMER_RIGHT_4=186,22
PRIMER_LEFT_4_TM=60.217
PRIMER_RIGHT_4_TM=59.835
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=129
=
PRIMER_SEQUENCE_ID=SSR_05184 Ps_contig_mira-and-tgicl-ass_franssen_36750
SEQUENCE=AGACGGTTACAACATTCCGGTCTCCGTCGTCGTTGTTTTTCTTAACAAGAACATAAATTCGAAGTGTAACATTCAAGGTTGTTTGAAGGATGTTAGAAGTTTGTGTCACGTGAGCTTGAGGTTTTGAATTCTAATGGAGAAGTTGTGGCTTGCAAAAGTGCTTGTTTGGCTTTTGATCTTGATAACTTTTGTTGTAGGAATGGTTATGGAATCCTGAGAAGTGCAAGCCAAATGTTTACTCAAAAATATTTAAGGATGCTTGCCCTAATTATTTTAGTTATGCTTTTGATACTCCTACTCCTCTTGTGAGTTGTGGATCCTTAGAATATGTAATCACATTTTGTCCTAATGGGTGGGGTCGTGCTGCCGTTGATGTGCCAAAACCTCGTATTGAAGTTTCTGATATATGATGCGTGTCAGTGCGTATCGGTATAACATGTCTGTCAGATGTCTGAGTTTGCGTCTATATTTGATATTGTTTATTTTTCTATTTATTTTGTTTTATTTTATTTTATTTTATTTTTTATTCTTTNACRCGTGATAGTGGAAGTTCGGTTGGATAGAATCAAATTAACAATTAGAAAATTTAAAA
TARGET=500,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05185 Ps_contig_mira-and-tgicl-ass_franssen_36751
SEQUENCE=ATACTACACTATACTTACCCTTTCCTACCACTATTATTATTATTATTTCCACATACCATACAAATTATTCACTTGAATGCTTAGAGAGAGAAAGAGTGATAACTGATCATGGAGGTTGTTGGCATGAAGAAGGAGAATATGAGATTTGAAGAAACTGAGCTGAGACTTGGAATTGGATCAGCCATAGTTTCTGAAGAAGTTGTAAGGAAGAGAGGATTTTCTGAGACTGAAACTCATCATGATGATGAGAAAACTACCATGGATTTGATGCTTAATCTTTCCTCTAAGGAAGCTACTGCTGAGGTAGATCCAAATCATAAGAGCACCAAGACTTTGCCAAAGGAGAAGACTCTCTTGCTTGCAGATCCAGCTAAACCTCCTACAAAGACGCAAGTGGTAGGTTGGCCACCTGTGAGGTCGTTCAGGAAGAACATGTTAGCAACACAAAAGAGTGAAGAAAGTGGCAATCTAAATCACATTGCTATTAGCTTTGTGAAAGTTAGCATGGATGGAGCACCTTACCTCCGTAAAGTAGACTTGAAGATGTACAAAAACTACTCCCAGCTTTCCGATTCCTTAGGCAAAATGTTCAGCTCCTTCACCATTGGAAACTGTGAATCTCAAGGCATGAAGGATTTTATGAATGAGAGTAAGTTGATGGATCTTTTAAACAACTCTGATTATGTCCCAACCTACGAAGACAAGGATGGTGACTGGATGCTTGTCGGTGACGTTCCATGGGAGATGTTTGTTGAATCATGCAAACGTCTCCGTATCATGAAAGGAAAGGAGGCTATTGGTATCGCACCAAGAGCTATGGAGAAATGCAAAAACAGGAGCTAGTTTACCACCTGCTCCATCCTTTATCATATTTTGATGCCGGTTTGAATTATAGTAAGATAATTAAGAGCACAGCAGCCACAAATTAATGGACATAACAGGATGTTCAATATAATTAATTTGTGTATTATTTATTATATGGTTTGTGTTGTTATATATATTGTTTAAAACAAAATATAATTAAGAGTCTTAAGTGCTGCAGTAAGTAGTAAGAACAGACACAATAAACGACTCTAAATTATATATATTTGATTTACCAATTAGTGTGTTTGCTAGCTTTAAAGTTTNATGATTCCTTNATATTAAATATTTAAAATTTAAANCAAGTTTAAAAAATTTT
TARGET=32,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05186 Ps_contig_mira-and-tgicl-ass_franssen_36754
SEQUENCE=TGGAAGGACAAGGACGAAATAGCAACCTACGATCAAGATAAATTAGTTGCCTTTATTGTTGCAGGTCACAATATGATTATCTCATGCCAGGTTTGTTACATTGAATGATAGACTTTGTATTATCATCTATGACTGCAATTYGGAATGCTCGATCGTCGTGTGTTATTACCTTTATACTGAAGTGCATTGATACTTGTAATGTTTCTCCTTTGATATGTGTTGCTTGACTAGGTTGAAAATTGATTGTGTAATATGCTCACTGATATGCCTTGCCTCAATTTCCTTTCACATGGGTTGCTCCTTATTGGTTTCAATCCTGTCTATTGGCTTTGTTTGTTGAATGCAGGATCTCATTGGCGGCATAGGTCCTTATTCCCTGCCTTGGTTGTGAAGCGGCTTACGTTTTGAATTGAGATTAGAGTGTGAGCATCTAAGAAGGGTGAAAACGATCGTGAATGTTAGCATTCGTCCGCTTTACGAGGCTTGGATTTGTTTTCGAGAAGGATTTCGGTTGAATTTCCCTTGTGAACCTTTACTTGTTAGCTTCTTGATTTGGGTTGAAAATCCAACTTTCTGTATCCATTGCCGGTGTTTGTTTTTATTGACTTTGGCTTGATCTCTTGGCTCTCATTAAGTCAATTGTTCATGCTGCTGGATTGTACATGTCTGTGTGTATGAATTACTATGAAGTAACCTTTACTCACACACTTGATTTGGCAGGTTGCACTTGTGAAATTAATTTGCAGTTTCAACAGTACTGAATGGATCTCCTTTTGCAAGCTTAAGTTTCTGCACGTCACTTAACTGGTGTGATGTTGATACTCTACTCCACGACGGTTTAACATCTATGCTACCGACCAAATGTCGATCCAGCTATTAACATCAACATTAGCACCGTCTGATATGGAGTTGTTACCCATCTGCATCATCAAAGGACAGTCATGTTGAAAAGGTCTTCAACATGGCCTGCTGCGGGAGGAGTACTCTGAGTTGCGTCCACAGCCGTCTTTTGGGTGTTACAGCATGTTGTTGTTGTTTTGTCATTCTGCAGCTTGTTGCATTTGTTTTCAAATTGTATTGCTTGGTCTATTCTTTTTGCCATGTAAAGTTGTTATCTTAAGTAGGTGAAATGAATAATTGTTTGAGTTGTTTGTTGGATTGTCTTGTTTGTAAATGAAACCCTGTTGTAAATTAATTTATGTCCTACTTAAAACAAACAGTTTGGAGTTGAGCCCTTGTAAAAAAACTAATCTATGTTATGAAAGATGATTGACATTTGGATTGTAACAGCTTATGGACTG
TARGET=1025,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05187 Ps_contig_mira-and-tgicl-ass_franssen_36755
SEQUENCE=CGGANRAAAGAAARAAAGGAAGAAAAAGTTGAGCACGAAAAAGAAGATGGAAAAACTCACTCACCTCACGTCTCTCTCTCTCCCCCACTCATTTTACTCCACCCACTCTTCTCTCTCTCTCTCTTTCATAATAATGACTAACCCTCTTCTCTTTCTGATCACTCAAATTCTCTTTTCAAAAAAATAATAAAACGAAAAGAACAAAACTCTTCATCTTCCTTTCTCTCCACCTCACCTTCTTCCATCACCATCACAAATCTGACTCTCCAAGTAAATCTTCATCTTCGCTTCTCCACCTTTGTTTAACCTAACGAAACACCGCGCGCCGCCGCGATTACATGCACAACCAAGCACTTCCCTCCTCTCACACACCCTTCACGATAACCATCCTTCATTTTCCGATAACCCAAACCCTCAGTCACCGGCGAAACCCTCAGAGCAACCACCATCTCCGTCCGCGTCAAAGCAACGACCTCCATACCTCCGTCCTCCATCGAAATGCGCCACGATCCGAATCACACAAACAACCTTCACAACCATGACAATACCACATCCCTTGATGTTTTCTAGATGATTGAAAGCGTTTTAAAATTATCTACTTCCTAGATTTGGTTGTTGATGGTCCGATGGCGACTTCAATGCACAATAGAGCTTTGAGCTCAATCCATGGACATTTCTGCTTGGGCGAGAACAAGGGAGAAAACCTCATGTTTTCTGGAAGGACGTGAGCAATAAAGCACAGAAGCAACCACATCAATCCTTGGACAATCGGTAATGTTTAAGTTCGTATGTTGTATAGATTAAATTCAAGATTTGTTTTCTGTTTAAGCTATTTTAGTGAATTGAGATTGGAGTTTTCATGTTGAAAACTTTGAGTTGATTGTTTGATATTCATTTCGACTGGGTGTGTTTGCCAAATTTGTTCGGGCGTGAGCTTATTGTGAGTTTTATTATGAGTTGCTTATTTGATATTCCACATTGTAGTGATGGTTACAGGTTATGAAATATTGGTTCATGTTGCAAATGTATGGTGAGGCGTTAGAAAATATGTTGTCTTGACCAACTACTTGTGTATTATTGTCGACCAAGTAAATAGAAAGTAAGTTAAGGAAAAAG
TARGET=71,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05188 Ps_contig_mira-and-tgicl-ass_franssen_36755
SEQUENCE=CGGANRAAAGAAARAAAGGAAGAAAAAGTTGAGCACGAAAAAGAAGATGGAAAAACTCACTCACCTCACGTCTCTCTCTCTCCCCCACTCATTTTACTCCACCCACTCTTCTCTCTCTCTCTCTTTCATAATAATGACTAACCCTCTTCTCTTTCTGATCACTCAAATTCTCTTTTCAAAAAAATAATAAAACGAAAAGAACAAAACTCTTCATCTTCCTTTCTCTCCACCTCACCTTCTTCCATCACCATCACAAATCTGACTCTCCAAGTAAATCTTCATCTTCGCTTCTCCACCTTTGTTTAACCTAACGAAACACCGCGCGCCGCCGCGATTACATGCACAACCAAGCACTTCCCTCCTCTCACACACCCTTCACGATAACCATCCTTCATTTTCCGATAACCCAAACCCTCAGTCACCGGCGAAACCCTCAGAGCAACCACCATCTCCGTCCGCGTCAAAGCAACGACCTCCATACCTCCGTCCTCCATCGAAATGCGCCACGATCCGAATCACACAAACAACCTTCACAACCATGACAATACCACATCCCTTGATGTTTTCTAGATGATTGAAAGCGTTTTAAAATTATCTACTTCCTAGATTTGGTTGTTGATGGTCCGATGGCGACTTCAATGCACAATAGAGCTTTGAGCTCAATCCATGGACATTTCTGCTTGGGCGAGAACAAGGGAGAAAACCTCATGTTTTCTGGAAGGACGTGAGCAATAAAGCACAGAAGCAACCACATCAATCCTTGGACAATCGGTAATGTTTAAGTTCGTATGTTGTATAGATTAAATTCAAGATTTGTTTTCTGTTTAAGCTATTTTAGTGAATTGAGATTGGAGTTTTCATGTTGAAAACTTTGAGTTGATTGTTTGATATTCATTTCGACTGGGTGTGTTTGCCAAATTTGTTCGGGCGTGAGCTTATTGTGAGTTTTATTATGAGTTGCTTATTTGATATTCCACATTGTAGTGATGGTTACAGGTTATGAAATATTGGTTCATGTTGCAAATGTATGGTGAGGCGTTAGAAAATATGTTGTCTTGACCAACTACTTGTGTATTATTGTCGACCAAGTAAATAGAAAGTAAGTTAAGGAAAAAG
TARGET=110,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05189 Ps_contig_mira-and-tgicl-ass_franssen_36757
SEQUENCE=CGGGGAAARNAAGCTTCCTCGCGTTCCAACATACTCTCTCTCTTGGGGATCTTGGGTCGTCGACTCCGCCGCGCCAGAAATCGTCTCTGGCGGCGGCGGAGGCGGAGGCGGAGGCTCAAACACTCCAATTCATACCTCATCTTGTCCCTCTCTTTCTTCTCTATCTAAACCCTAACCTAATTTTCCCCAATTTCCCCTTAATAAACTAAATCGAAGCTCACAGTGGATGAACCCTAAGAATCCTTGAACTCTAATTAGACGTTAAACTCACTCGAACGTGACTAGCAAACGTATGATCGTGGAGATTCTACGCTAGTGCAATTGTTTGGTAGAAGAAGAAAGAAAGATAGATGAAAGATGGTTAACCGACCTTCGAAGTTGTGAGCTGCTTCGGTAAATGTTACAAGTCTCAAAGGTGCAGCTCGAACAAGAATCCCAGCGATCTCCACTGAGGTATGGACTGCAGGGCAAGGCAGGGCTTTGCAATTTGCAGCTATAATGGTGGAATGGCCTGCTGCTAAGTTGTT
TARGET=34,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05190 Ps_contig_mira-and-tgicl-ass_franssen_36757
SEQUENCE=CGGGGAAARNAAGCTTCCTCGCGTTCCAACATACTCTCTCTCTTGGGGATCTTGGGTCGTCGACTCCGCCGCGCCAGAAATCGTCTCTGGCGGCGGCGGAGGCGGAGGCGGAGGCTCAAACACTCCAATTCATACCTCATCTTGTCCCTCTCTTTCTTCTCTATCTAAACCCTAACCTAATTTTCCCCAATTTCCCCTTAATAAACTAAATCGAAGCTCACAGTGGATGAACCCTAAGAATCCTTGAACTCTAATTAGACGTTAAACTCACTCGAACGTGACTAGCAAACGTATGATCGTGGAGATTCTACGCTAGTGCAATTGTTTGGTAGAAGAAGAAAGAAAGATAGATGAAAGATGGTTAACCGACCTTCGAAGTTGTGAGCTGCTTCGGTAAATGTTACAAGTCTCAAAGGTGCAGCTCGAACAAGAATCCCAGCGATCTCCACTGAGGTATGGACTGCAGGGCAAGGCAGGGCTTTGCAATTTGCAGCTATAATGGTGGAATGGCCTGCTGCTAAGTTGTT
TARGET=336,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05191 Ps_contig_mira-and-tgicl-ass_franssen_36758
SEQUENCE=GTCGGGGTATCCCCTCAAACATTCGGGCATCTGTGAACCCACGGGTTCAAAGATAAAAGACCAAAATTTCCGGTTTTTCTTTTTTGGCTACCATTACACTACCGGATAACAGGACGTTGGCTGTACTTGCACTCCCCCTCATATCCAGTATGACAATGGTGCTAAGAAGAGGAGCGATGACGCTCACTTTCCTATTCCAAACCCCTCCTCCTTCTCTCTATCTCTCCCACACTTCTTCTCTCTCCATTCCCACAACCACCACCACCACCACCCCTTCTCTTCTCTCTCTCCGACACTCTTCAATTCCCGCTTCCAAAACCCTAACCCCTTCTCTCCCCCCTTCCGAGAAACCCCTCTTATTCATCCCACCAGGAGTTGAACCCACCCAAGTCAACGACTCCATGATTCTCCCTTTCTCTAACATCGTCGTCGGACCCTACGCCGGCGAATCTCGAATCAAGGATGTCCAATTCGTTAAGAGTAGTCCCCGGGCAAAAGATTGCCCTAAAGATGACCGACCTGAATTCGCCATTCTTGGTCGTTCTAATGTTGGGAAGTCTTCTCTTATCAATTCTCTTGTTCGTAAGAAAGAACTCGCCCTCACTTCTAAGAAACCAGGGAAGACACAGCTTATCAATCACTTCTTGGTCAACAAGAGCTGGTACCTTGTGGATTTACCTGGTTATGGCTTTGCTAAAGCATCTGAATCAGCTAAAACAGACTGGTCTTCGTTTACAAAAGGTTACTTTTTAAATAGAAATACGCTAGTATCTGTCTTGCTTCTCATTGATGCAAGCGTTCCGCCTCAAAGGATTGACTTCGATTGTGCAAATTGGCTTGGACGCAACGATATACCAATTACCTTTGTTTTCACTAAATGTGACAAAATGAAGGTGGCGAAGGGAAAGCGGCCTGATGAAAACATAAAAGAATTTCAAGAGATAATAAAACAAAACTACAAACAACATCCCCCGTGGATTATGACCAGCGGTGTCACTGGATTGGGCAGAGATGAGCTTCTCTTGCATGTATCTCAGCTAAGAAACTACTGGGATCAATAGGAGAGCAACTTAAAACAAGCAGAATGTGAATATCTATAAGAAGAGCATTCCATGCTGGAGCAACATTGAAATAACCAAAGATGCAATCACATAGAAGAGCCATGTGAAAAAGGATATGGGCTGACTTGTATTTCAGCTGAATCATTGTTCCACTCTGACCACAACCTGATATATATGCTATCTAAATCTTACACGTGGGTGAGTTTGAGTTGTTTGTATACTGGAATTTTACACTCTGTCATGTTGTAGTTCTAAGCATGTGGGAATAATACAGTATAAGGAAAATAATACAATACTTTCAACTGTGAGTTTATGTATTTTATTCTTTTTTAATACTAAAAACGG
TARGET=255,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1638
PRIMER_LEFT_PENALTY=0.129103
PRIMER_RIGHT_PENALTY=0.034674
PRIMER_LEFT_SEQUENCE=CGCTCACTTTCCTATTCCAAAC
PRIMER_RIGHT_SEQUENCE=AATAAGAGGGGTTTCTCGGAAG
PRIMER_LEFT=180,22
PRIMER_RIGHT=360,22
PRIMER_LEFT_TM=60.129
PRIMER_RIGHT_TM=59.965
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.1638
PRIMER_LEFT_1_PENALTY=0.129103
PRIMER_RIGHT_1_PENALTY=0.034674
PRIMER_LEFT_1_SEQUENCE=ACGCTCACTTTCCTATTCCAAA
PRIMER_RIGHT_1_SEQUENCE=AATAAGAGGGGTTTCTCGGAAG
PRIMER_LEFT_1=179,22
PRIMER_RIGHT_1=360,22
PRIMER_LEFT_1_TM=60.129
PRIMER_RIGHT_1_TM=59.965
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=0.1740
PRIMER_LEFT_2_PENALTY=0.129103
PRIMER_RIGHT_2_PENALTY=0.044904
PRIMER_LEFT_2_SEQUENCE=CGCTCACTTTCCTATTCCAAAC
PRIMER_RIGHT_2_SEQUENCE=CTCCTGGTGGGATGAATAAGAG
PRIMER_LEFT_2=180,22
PRIMER_RIGHT_2=374,22
PRIMER_LEFT_2_TM=60.129
PRIMER_RIGHT_2_TM=59.955
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.1740
PRIMER_LEFT_3_PENALTY=0.129103
PRIMER_RIGHT_3_PENALTY=0.044904
PRIMER_LEFT_3_SEQUENCE=ACGCTCACTTTCCTATTCCAAA
PRIMER_RIGHT_3_SEQUENCE=CTCCTGGTGGGATGAATAAGAG
PRIMER_LEFT_3=179,22
PRIMER_RIGHT_3=374,22
PRIMER_LEFT_3_TM=60.129
PRIMER_RIGHT_3_TM=59.955
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=0.2595
PRIMER_LEFT_4_PENALTY=0.129103
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=ACGCTCACTTTCCTATTCCAAA
PRIMER_RIGHT_4_SEQUENCE=GACGACGATGTTAGAGAAAGGG
PRIMER_LEFT_4=179,22
PRIMER_RIGHT_4=430,22
PRIMER_LEFT_4_TM=60.129
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_05192 Ps_contig_mira-and-tgicl-ass_franssen_36758
SEQUENCE=GTCGGGGTATCCCCTCAAACATTCGGGCATCTGTGAACCCACGGGTTCAAAGATAAAAGACCAAAATTTCCGGTTTTTCTTTTTTGGCTACCATTACACTACCGGATAACAGGACGTTGGCTGTACTTGCACTCCCCCTCATATCCAGTATGACAATGGTGCTAAGAAGAGGAGCGATGACGCTCACTTTCCTATTCCAAACCCCTCCTCCTTCTCTCTATCTCTCCCACACTTCTTCTCTCTCCATTCCCACAACCACCACCACCACCACCCCTTCTCTTCTCTCTCTCCGACACTCTTCAATTCCCGCTTCCAAAACCCTAACCCCTTCTCTCCCCCCTTCCGAGAAACCCCTCTTATTCATCCCACCAGGAGTTGAACCCACCCAAGTCAACGACTCCATGATTCTCCCTTTCTCTAACATCGTCGTCGGACCCTACGCCGGCGAATCTCGAATCAAGGATGTCCAATTCGTTAAGAGTAGTCCCCGGGCAAAAGATTGCCCTAAAGATGACCGACCTGAATTCGCCATTCTTGGTCGTTCTAATGTTGGGAAGTCTTCTCTTATCAATTCTCTTGTTCGTAAGAAAGAACTCGCCCTCACTTCTAAGAAACCAGGGAAGACACAGCTTATCAATCACTTCTTGGTCAACAAGAGCTGGTACCTTGTGGATTTACCTGGTTATGGCTTTGCTAAAGCATCTGAATCAGCTAAAACAGACTGGTCTTCGTTTACAAAAGGTTACTTTTTAAATAGAAATACGCTAGTATCTGTCTTGCTTCTCATTGATGCAAGCGTTCCGCCTCAAAGGATTGACTTCGATTGTGCAAATTGGCTTGGACGCAACGATATACCAATTACCTTTGTTTTCACTAAATGTGACAAAATGAAGGTGGCGAAGGGAAAGCGGCCTGATGAAAACATAAAAGAATTTCAAGAGATAATAAAACAAAACTACAAACAACATCCCCCGTGGATTATGACCAGCGGTGTCACTGGATTGGGCAGAGATGAGCTTCTCTTGCATGTATCTCAGCTAAGAAACTACTGGGATCAATAGGAGAGCAACTTAAAACAAGCAGAATGTGAATATCTATAAGAAGAGCATTCCATGCTGGAGCAACATTGAAATAACCAAAGATGCAATCACATAGAAGAGCCATGTGAAAAAGGATATGGGCTGACTTGTATTTCAGCTGAATCATTGTTCCACTCTGACCACAACCTGATATATATGCTATCTAAATCTTACACGTGGGTGAGTTTGAGTTGTTTGTATACTGGAATTTTACACTCTGTCATGTTGTAGTTCTAAGCATGTGGGAATAATACAGTATAAGGAAAATAATACAATACTTTCAACTGTGAGTTTATGTATTTTATTCTTTTTTAATACTAAAAACGG
TARGET=281,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1448
PRIMER_LEFT_PENALTY=0.110131
PRIMER_RIGHT_PENALTY=0.034674
PRIMER_LEFT_SEQUENCE=CTTCTCTCTCCATTCCCACAAC
PRIMER_RIGHT_SEQUENCE=AATAAGAGGGGTTTCTCGGAAG
PRIMER_LEFT=234,22
PRIMER_RIGHT=360,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.965
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=127
PRIMER_PAIR_PENALTY_1=0.1638
PRIMER_LEFT_1_PENALTY=0.129103
PRIMER_RIGHT_1_PENALTY=0.034674
PRIMER_LEFT_1_SEQUENCE=CGCTCACTTTCCTATTCCAAAC
PRIMER_RIGHT_1_SEQUENCE=AATAAGAGGGGTTTCTCGGAAG
PRIMER_LEFT_1=180,22
PRIMER_RIGHT_1=360,22
PRIMER_LEFT_1_TM=60.129
PRIMER_RIGHT_1_TM=59.965
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=181
PRIMER_PAIR_PENALTY_2=0.1638
PRIMER_LEFT_2_PENALTY=0.129103
PRIMER_RIGHT_2_PENALTY=0.034674
PRIMER_LEFT_2_SEQUENCE=ACGCTCACTTTCCTATTCCAAA
PRIMER_RIGHT_2_SEQUENCE=AATAAGAGGGGTTTCTCGGAAG
PRIMER_LEFT_2=179,22
PRIMER_RIGHT_2=360,22
PRIMER_LEFT_2_TM=60.129
PRIMER_RIGHT_2_TM=59.965
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=182
PRIMER_PAIR_PENALTY_3=0.1740
PRIMER_LEFT_3_PENALTY=0.129103
PRIMER_RIGHT_3_PENALTY=0.044904
PRIMER_LEFT_3_SEQUENCE=CGCTCACTTTCCTATTCCAAAC
PRIMER_RIGHT_3_SEQUENCE=CTCCTGGTGGGATGAATAAGAG
PRIMER_LEFT_3=180,22
PRIMER_RIGHT_3=374,22
PRIMER_LEFT_3_TM=60.129
PRIMER_RIGHT_3_TM=59.955
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=0.1740
PRIMER_LEFT_4_PENALTY=0.129103
PRIMER_RIGHT_4_PENALTY=0.044904
PRIMER_LEFT_4_SEQUENCE=ACGCTCACTTTCCTATTCCAAA
PRIMER_RIGHT_4_SEQUENCE=CTCCTGGTGGGATGAATAAGAG
PRIMER_LEFT_4=179,22
PRIMER_RIGHT_4=374,22
PRIMER_LEFT_4_TM=60.129
PRIMER_RIGHT_4_TM=59.955
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=196
=
PRIMER_SEQUENCE_ID=SSR_05193 Ps_contig_mira-and-tgicl-ass_franssen_36764
SEQUENCE=AAGAAAGGCAGTTCTACATTTTATTTAACCCGGGCTTGGCAAAACTCAAAAACACTAGTTCAAAATAGCAGAAACAAATGAACATCAGAATAAATAATTAATTACCACCAGACTGTACATTTCACTTCAATAAAACCATAACTAAGAGCTAACCTTCCCACCAATAAAATCCAAATGAGAACTATGAACAACACTCAAATCTTACCCTAACACCACCAAAATCCAAGGCTACCTCTTGCTTTCCTTTCCTTACGCTAAATTATTTTATTTTATTGTTTTTCTCCTTATTTCGCCCCTTTGGCTTACCAACTGCCATCCAAAATTGCCTAAAAGGTAAAATTGAACATTCACTTCTCTTTTATTCTGTGCATTTGCTTTGCTTTGCTTTAGGACCTTGAAAAAACAAACATGCGTCCTAAGTCATCACTGTGTGTTTGCTGCTGCCCTTGTGCTTGTTGTTGAGCTCATGTTGCCTCTGGACAAATTCATCCTGATTCCCATCAATACTCTGGTCTTCTTTGGTGAGGTAGCTGTCAACTTTGGAAGCAGCTTGTCTTCCCTCTGAAATAGCCCATACAACCAACGACTGACCCCGCCGACAGTCCCCAGCTGCAAATACCCCATCAACATTGGTTGAGAAACGGCCATAATCAGCCTTGAAGTTTGACCTGTTGTCTTGCTCCACGCCCAGCTTTTCTGCAATAGTCGACTCAGGGCCAAGGAATCCCATAGCAAGTAAAACAAGGTCAGCCTCGATTATCTCCTCACTGCCCTCAATTTCCTTAAATTGAAACTTGCCTGTTTCATCCTTCTCCCATCGAACATGTACCACTTCAAGTCCTTTTACATTACCATTTTCATCTCCTACAAACCGCTTAGTCAGCACCTCGTACGTTCTTGGATCTTTGCCAAATTTAGTTTCCGCTTCTTGGTGGCCGTAATCAACACGGAATATGCGAGGCCACTGAGGCCATGGGTTGCCTGGGGCCCTAGTGGGTGGTGGCTGAGGGAGAAGTTCAAGATTTACGACGGCAGTACAACCATGACAAATGGATGTTCCAATGCAATCTGTGCCAGTGTCACCCCCACCAATGACCACAACCTTCTTGCCCTTGGCGGATATGAAGTTACCATCCTGTAAGTTGCTATCAAGCAAGCTTTTAGTGTTGGCATGAAGAAACTTCATGGCAAAATGGACTCCTGATAGCTCCCTTCCAGGTACAGGAAGATCCCTTGGCTTTGTGGCACCAACAGCCAAGACAATGGCATCATTTTCCTCTCGAAGCTGTTCAAGAGAATATCGAGGATCATTTCCAATATTTG
TARGET=260,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1213
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.101479
PRIMER_LEFT_SEQUENCE=TACCCTAACACCACCAAAATCC
PRIMER_RIGHT_SEQUENCE=TTTCAAGGTCCTAAAGCAAAGC
PRIMER_LEFT=202,22
PRIMER_RIGHT=399,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=59.899
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=198
PRIMER_PAIR_PENALTY_1=0.2237
PRIMER_LEFT_1_PENALTY=0.122257
PRIMER_RIGHT_1_PENALTY=0.101479
PRIMER_LEFT_1_SEQUENCE=TAAGAGCTAACCTTCCCACCAA
PRIMER_RIGHT_1_SEQUENCE=TTTCAAGGTCCTAAAGCAAAGC
PRIMER_LEFT_1=142,22
PRIMER_RIGHT_1=399,22
PRIMER_LEFT_1_TM=60.122
PRIMER_RIGHT_1_TM=59.899
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.3237
PRIMER_LEFT_2_PENALTY=0.222249
PRIMER_RIGHT_2_PENALTY=0.101479
PRIMER_LEFT_2_SEQUENCE=CTAAGAGCTAACCTTCCCACCA
PRIMER_RIGHT_2_SEQUENCE=TTTCAAGGTCCTAAAGCAAAGC
PRIMER_LEFT_2=141,22
PRIMER_RIGHT_2=399,22
PRIMER_LEFT_2_TM=59.778
PRIMER_RIGHT_2_TM=59.899
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.3826
PRIMER_LEFT_3_PENALTY=0.019805
PRIMER_RIGHT_3_PENALTY=0.362762
PRIMER_LEFT_3_SEQUENCE=TACCCTAACACCACCAAAATCC
PRIMER_RIGHT_3_SEQUENCE=TGATGACTTAGGACGCATGTTT
PRIMER_LEFT_3=202,22
PRIMER_RIGHT_3=425,22
PRIMER_LEFT_3_TM=59.980
PRIMER_RIGHT_3_TM=59.637
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.3843
PRIMER_LEFT_4_PENALTY=0.019805
PRIMER_RIGHT_4_PENALTY=0.364479
PRIMER_LEFT_4_SEQUENCE=TACCCTAACACCACCAAAATCC
PRIMER_RIGHT_4_SEQUENCE=ACATGAGCTCAACAACAAGCAC
PRIMER_LEFT_4=202,22
PRIMER_RIGHT_4=469,22
PRIMER_LEFT_4_TM=59.980
PRIMER_RIGHT_4_TM=60.364
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_05194 Ps_contig_mira-and-tgicl-ass_franssen_36764
SEQUENCE=AAGAAAGGCAGTTCTACATTTTATTTAACCCGGGCTTGGCAAAACTCAAAAACACTAGTTCAAAATAGCAGAAACAAATGAACATCAGAATAAATAATTAATTACCACCAGACTGTACATTTCACTTCAATAAAACCATAACTAAGAGCTAACCTTCCCACCAATAAAATCCAAATGAGAACTATGAACAACACTCAAATCTTACCCTAACACCACCAAAATCCAAGGCTACCTCTTGCTTTCCTTTCCTTACGCTAAATTATTTTATTTTATTGTTTTTCTCCTTATTTCGCCCCTTTGGCTTACCAACTGCCATCCAAAATTGCCTAAAAGGTAAAATTGAACATTCACTTCTCTTTTATTCTGTGCATTTGCTTTGCTTTGCTTTAGGACCTTGAAAAAACAAACATGCGTCCTAAGTCATCACTGTGTGTTTGCTGCTGCCCTTGTGCTTGTTGTTGAGCTCATGTTGCCTCTGGACAAATTCATCCTGATTCCCATCAATACTCTGGTCTTCTTTGGTGAGGTAGCTGTCAACTTTGGAAGCAGCTTGTCTTCCCTCTGAAATAGCCCATACAACCAACGACTGACCCCGCCGACAGTCCCCAGCTGCAAATACCCCATCAACATTGGTTGAGAAACGGCCATAATCAGCCTTGAAGTTTGACCTGTTGTCTTGCTCCACGCCCAGCTTTTCTGCAATAGTCGACTCAGGGCCAAGGAATCCCATAGCAAGTAAAACAAGGTCAGCCTCGATTATCTCCTCACTGCCCTCAATTTCCTTAAATTGAAACTTGCCTGTTTCATCCTTCTCCCATCGAACATGTACCACTTCAAGTCCTTTTACATTACCATTTTCATCTCCTACAAACCGCTTAGTCAGCACCTCGTACGTTCTTGGATCTTTGCCAAATTTAGTTTCCGCTTCTTGGTGGCCGTAATCAACACGGAATATGCGAGGCCACTGAGGCCATGGGTTGCCTGGGGCCCTAGTGGGTGGTGGCTGAGGGAGAAGTTCAAGATTTACGACGGCAGTACAACCATGACAAATGGATGTTCCAATGCAATCTGTGCCAGTGTCACCCCCACCAATGACCACAACCTTCTTGCCCTTGGCGGATATGAAGTTACCATCCTGTAAGTTGCTATCAAGCAAGCTTTTAGTGTTGGCATGAAGAAACTTCATGGCAAAATGGACTCCTGATAGCTCCCTTCCAGGTACAGGAAGATCCCTTGGCTTTGTGGCACCAACAGCCAAGACAATGGCATCATTTTCCTCTCGAAGCTGTTCAAGAGAATATCGAGGATCATTTCCAATATTTG
TARGET=371,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2975
PRIMER_LEFT_PENALTY=0.236697
PRIMER_RIGHT_PENALTY=0.060824
PRIMER_LEFT_SEQUENCE=CTTACCAACTGCCATCCAAAAT
PRIMER_RIGHT_SEQUENCE=GAAGACAAGCTGCTTCCAAAGT
PRIMER_LEFT=301,22
PRIMER_RIGHT=557,22
PRIMER_LEFT_TM=60.237
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.3230
PRIMER_LEFT_1_PENALTY=0.236697
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=CTTACCAACTGCCATCCAAAAT
PRIMER_RIGHT_1_SEQUENCE=TGACAGCTACCTCACCAAAGAA
PRIMER_LEFT_1=301,22
PRIMER_RIGHT_1=535,22
PRIMER_LEFT_1_TM=60.237
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.3230
PRIMER_LEFT_2_PENALTY=0.236697
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=CTTACCAACTGCCATCCAAAAT
PRIMER_RIGHT_2_SEQUENCE=TTGACAGCTACCTCACCAAAGA
PRIMER_LEFT_2=301,22
PRIMER_RIGHT_2=536,22
PRIMER_LEFT_2_TM=60.237
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.3230
PRIMER_LEFT_3_PENALTY=0.236697
PRIMER_RIGHT_3_PENALTY=0.086339
PRIMER_LEFT_3_SEQUENCE=CTTACCAACTGCCATCCAAAAT
PRIMER_RIGHT_3_SEQUENCE=TCCAAAGTTGACAGCTACCTCA
PRIMER_LEFT_3=301,22
PRIMER_RIGHT_3=543,22
PRIMER_LEFT_3_TM=60.237
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.3512
PRIMER_LEFT_4_PENALTY=0.236697
PRIMER_RIGHT_4_PENALTY=0.114470
PRIMER_LEFT_4_SEQUENCE=CTTACCAACTGCCATCCAAAAT
PRIMER_RIGHT_4_SEQUENCE=ATTTCAGAGGGAAGACAAGCTG
PRIMER_LEFT_4=301,22
PRIMER_RIGHT_4=567,22
PRIMER_LEFT_4_TM=60.237
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_05195 Ps_contig_mira-and-tgicl-ass_franssen_36768
SEQUENCE=TCGGGGTAACCTTATAAAACACAAAATGAGTGAAACGTAGGNNGCCCTTTCTTCTTTTGGGCTGTGGATAATTTGAACCAATAGTGTATGGTCAATTACAGCGAATAGATAGCGTGATCAAGAGTTAAGCGAATGGAAGTCACAACAGTAAGGAGCTTCGGTTTCAGTATCACTCATTCTTTTCCACCGAGAAGGAAGTTTCATCCAACATTGGCTAAAAAGAAAGATAACGAGTCTCAACAACAACAACCACCTTTTTCATCATTGAGGATTTCGAACTCCAATCTTGCTCGCGCTGCCATTGGAGTGTTTGGATTAGGGTTCATTGATGCAGGGTATAGTGGAGACTGGTCAAGGATTGGAGTAATTACACCGCAGAATGAGGAATTTCTAAGGCTTGCAGCTTTTCTAGTGGTTCCCATTTGTGTTCTTTTTGTTTTTAGGGTTGCCAAAGAACCTGATTATTGAGCTTTTCAAATGCTATATGTAAATCTGTGAGTTTTAATTGTCGATAATTATATTCTTTATCCTGTTTTTCTCAGTATTCAAAATTCAATTGGTTGCGAAATTCACAATAATCCATGTAAAGAAAATAAAGAAAAACTTTGAAAGTTCAAGCTACTTNAATTTAAAACCTAA
TARGET=238,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2329
PRIMER_LEFT_PENALTY=0.137489
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=GGAGCTTCGGTTTCAGTATCAC
PRIMER_RIGHT_SEQUENCE=TAGAAATTCCTCATTCTGCGGT
PRIMER_LEFT=151,22
PRIMER_RIGHT=392,22
PRIMER_LEFT_TM=60.137
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=11.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.2399
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=ACTCATTCTTTTCCACCGAGAA
PRIMER_RIGHT_1_SEQUENCE=AAAGAACACAAATGGGAACCAC
PRIMER_LEFT_1=171,22
PRIMER_RIGHT_1=432,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.2569
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.147383
PRIMER_LEFT_2_SEQUENCE=ACTCATTCTTTTCCACCGAGAA
PRIMER_RIGHT_2_SEQUENCE=GAAAAGCTGCAAGCCTTAGAAA
PRIMER_LEFT_2=171,22
PRIMER_RIGHT_2=408,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.147
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.2678
PRIMER_LEFT_3_PENALTY=0.137489
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=GGAGCTTCGGTTTCAGTATCAC
PRIMER_RIGHT_3_SEQUENCE=AAAGAACACAAATGGGAACCAC
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=432,22
PRIMER_LEFT_3_TM=60.137
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=0.2711
PRIMER_LEFT_4_PENALTY=0.175676
PRIMER_RIGHT_4_PENALTY=0.095445
PRIMER_LEFT_4_SEQUENCE=GCGAATGGAAGTCACAACAGTA
PRIMER_RIGHT_4_SEQUENCE=TAGAAATTCCTCATTCTGCGGT
PRIMER_LEFT_4=128,22
PRIMER_RIGHT_4=392,22
PRIMER_LEFT_4_TM=60.176
PRIMER_RIGHT_4_TM=60.095
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_05196 Ps_contig_mira-and-tgicl-ass_franssen_36769
SEQUENCE=GGTTYGTGCAAAAGTACACACTTTTATATCTATATTTAATCAAACCAAATAAACAAATATGAACCGACAGGCCGAAAACAACACAGAAAGGGGTCACTCAGTCTCCGACCCTATTTGATAGAACTTTCTATGATACATGAATGCAAGACTTCCTTGGCATAACTAATCACTTTACGAGCTTAACATCCGGGTTCCTAATGGTTTTGAGCCACATATCAGCCACGATCCGTGATGAAATACTATATAGCAGGTCTTGTCCCTTTGCCGCTGCCTGTTTCCCTTTCTTCCCTGCCTTTCCCAACCCATATGCAACTAACCATTGCATTGCGGTTAGTCTCTGCTTCTCGTTGTCCCATCCGTTCTTCTTTGTAGAACTCCTAACCTTGAAACCTCCCACATGCATGCTTATTAGTTTGAACCTCTTCTCCTCCTCCTCAGTCCTGTTCGCGCCAACACGCGATGCATGAATAAGCACCATCACAGGGCCTCCAACTGTTTCATAGCGTCTCATTGGATCCCGCAGTTGGACAACCAATATCAGTGTGACTTTCTCAGGTTCGCCATCAGAACTTGCAGTTGCAGAGCCCTTGTTGTTGCCTAAGCTTTGGTTTCTAATCCAATCCTCAAGTGGAATAGCAGAAGCCAAAAGGTCGTTCCCGCTAACCCCTTTCTTTGAGCTGAGTGCAGAAAAATCAAATGGAGCTTCTTCCTCGGCCATGCCAGCTTGAATTTTCAGTGCTTCAACTGTCATAGACTCTATCTTCTGCATTGAAATAGGAAGAAGCTTCTCGGAAGTAACTGGATCTTCATCAACATTCCAAAGTCCCGTTGTTATCCGTTCTTTCCTTCCCGAACTGATAATATTTGACATGCTTTTCAAAGCAGAAACTATGCGGGCAGCGCTGGAACTTGCTCCTTCCTTGTTCCTACCTTGTACGACAGTTGAAGCAATGCCTTCAAAAGCAATCTGCTCTGCAGTTTTACCGATCAGTTCATCTATCGGCATCAAGGAGGATAAGACTTCAGAGCTGAGTTCATCAAGACCAAAGTGTCTCCACTGAAACCCATAAACAGGTCCTAAGTCACCCTCCTCCCTCTCTGACAAACCAATGCTATC
TARGET=425,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05197 Ps_contig_mira-and-tgicl-ass_franssen_36774
SEQUENCE=TCCAAAGGACACACTATCTCTTCCAAAAGCTAACTCTTCAATACAGAAATACTCTTATGACTCGAGCTACTCGAGGCCACATTCCGGTACCTCGTCTAAAGATCTTCATCAAAAAGACCTCAAAGCTTCCATCTTAAGCGAGCTAAAAAACGAACGCTGATCTTCAATTAGCAGCTTTACTAAGCAAAGGCGGGATCGAAAGCTTGGAGAGCATCGTGCGGATAAAGGAGGCAGAAGCTAAAATGTTCCAAACTAAAGCAGACGAAGCGAGGAGAGAAGCAGAAGGGTTTCAGAAGATGGTCAGTTTAAAGACTGCTCAGATGGAAGATGAGTATACAACAAAGCTTTCGAAACTGAGTCTGCATGAGACCGAGGAAACGCAGAGAAGGAAGTTGGATGACCTGAAAGTCGTGGAAAATTCCTACTTCGATTACTATAAAATGAAGAAGAGAATGCAAGATGAGATCGATGGTTTGTTGCAGAGAATGGAGGCAACAAAGCAGCAATGGATTTAACCTCTTTTTTTCCTTTCATATTATTATCATTATTATTATTATTATTCCGGTTTTTATTTTGTTTATTGAAGTTCTGCTCCGGTATTCTGACTTGTTTCCAGAATGGATCTATTTTTTTCGTATTTTTCGGTTTTAATAGTATTCCG
TARGET=544,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1298
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=GTCGTGGAAAATTCCTACTTCG
PRIMER_RIGHT_SEQUENCE=CCGGAGCAGAACTTCAATAAAC
PRIMER_LEFT=407,22
PRIMER_RIGHT=596,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=0.1298
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=GTCGTGGAAAATTCCTACTTCG
PRIMER_RIGHT_1_SEQUENCE=ACCGGAGCAGAACTTCAATAAA
PRIMER_LEFT_1=407,22
PRIMER_RIGHT_1=597,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.1527
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=AAGGAAGTTGGATGACCTGAAA
PRIMER_RIGHT_2_SEQUENCE=CCGGAGCAGAACTTCAATAAAC
PRIMER_LEFT_2=385,22
PRIMER_RIGHT_2=596,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.1527
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.129103
PRIMER_LEFT_3_SEQUENCE=AAGGAAGTTGGATGACCTGAAA
PRIMER_RIGHT_3_SEQUENCE=ACCGGAGCAGAACTTCAATAAA
PRIMER_LEFT_3=385,22
PRIMER_RIGHT_3=597,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=60.129
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.2219
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.221115
PRIMER_LEFT_4_SEQUENCE=GTCGTGGAAAATTCCTACTTCG
PRIMER_RIGHT_4_SEQUENCE=AGTCAGAATACCGGAGCAGAAC
PRIMER_LEFT_4=407,22
PRIMER_RIGHT_4=606,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.779
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_05198 Ps_contig_mira-and-tgicl-ass_franssen_36780
SEQUENCE=CCAATACCTTCACAACATATGCGGTTCATATTTCTTGTCCTTCAAATCCTGNNNNGATATCCAAAATTTTCACTACATACTCACATTAAATCAACATGGACAAAGATTCAACAACCCTCTTCATCAACACCCTTCTTTTCTTCTTATGCTTCTCATTCTCACATTCTTCAACATCATTTCCCAAACAAGCACTTCCCACAAAATCAGGTTACCTTCCAGTCACCTCAACCTCCAATTCCTCCATTTTCTACACATTCTATGAAGCCCAAAACTCATCCTTATCTCTCTCCCAAACCCCACTTCTCATCTGGCTTCAAGGTGGTCCTGGCTGCTCCTCCATGATTGCCAACTTCTACGAACTCGGACCCYACCGTGTCACCAAATCACTCACCCTTCAACCCAACCCTGGTTCTTGGAACAGAATCTTTGGTCTTCTTTTTCTCGATAATCCAATTGGAACTGGCTTCAGTGTAGCCTCAACACCACAAGAGATTCCAACCGATCAAAACGGAGTGGCGAAACACCTTTTCGCCGCTATAACAAGGTTTGTTCAACTTGATCCTGTTTTCAAACATCGTCCTATTTATATCACCGGTGAAAGCTATGCTGGAAAATATGTTCCTGCAATTGGATACTACATATTGCAGAAAAATGCCGAGTTGAAAGAGTTGAAAGAGTCTCAGAGAGTGAATTTAGCTGGTTTGGCAATCGGNGATGGATTAACAGACCCTGTAACACAGGTTGTTACTCATGCTGCTAATGCTTATTACACTGGATTAATCAACGAGAAGCAAAAGAACGAGATGGAAAAAGCTCAGCTTGAAGCTGTTAGATTGGTTGAAAGAGGGAATTGGAGTGAGGCAACTGATGCTAGAAACAATGTATTGAGTTTACTGAGAAACATGACAGGGTTGGCTACTTTGTATGATTATTCAAGGAATGTTCCTTATGATGATGATCTGGTTGAAAAGTTATTGAACATTGCTGAAGTGAAGAAGGCGTTAGGAGTGAAAGTTGACGAATCGTTTGTTTATGAGATATGCAGTGATCTTGTTGGTGATGTATTGCATGATGATGTGATGAAGAGTGTTAAGTTTATGGTGGAGAAGTTGTTGAAAGAGAGTAGTACTAGGTTGTTGTTGTATCAAGGACAACGTGATTTGAGAGATGGTGTGGTTCAAGTTGAGGCTTGGGTGAAGACTATGAAATGGGAAGGGATTGTTGAGTTTTTGAATGGTGAGAGAGAGATTTGGAAGGTGAATGGAGATGTAGCTGGTTATGTGCAAAAATGGAAGACACTTACAAATGTTGTGGTTTTGGGAGGTGGACATCTTTTGCCTAATGACCAACCTTTGAATTCTCAAGCTATGATTGAAGATTGGGTTTTGGAAAAGGGTTTGTTTGGAAGTGTGCCTTATGTTAGTATGTCTTGAGTTGTCAGTTTTGATGATGTTTTTTGTTGTCGAAATTGTAATAAATGTGGAAATGCATGTTTTGCTTAAANTNAAGGTACNAACNTGGATTTGGTTTCTCTTTGAC
TARGET=1239,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05199 Ps_contig_mira-and-tgicl-ass_franssen_36784
SEQUENCE=CCTAAACCACTTGTCTNCNCTCTCGNRNCACAGCCAAACGCCCCACCCTTCTTCCCTCAACCGCGCCGTCGAAATCCCATCACCTCCGCCGTTCTCACGTGGGACCCTCACAAACACCTCCGTCTTCACGCACACCTCCGTGAAGTCTCAACCTCCCGACAACACAACCACAAACCGTCGGCTATTCTCCTTCCATTTCCTCCTTCAACCGCGTACCGCCGCCACAACCTCCAAACCCCCACCGCTCAAGCCACCACGCACAAACCTCAATAAAAATCCGGTGACGCCGCGTTTACAACCTCCTCCATTACATCGCCGCGCCGAATCCACCTCTCGCTAACCTTCAAGTTTCCGACCACCATAATTGTTTTCTTTAACTTCGTTCATTTATACAGGAAACAACAGTTTAACCACACACCTGTGCGGCCTCAGTTCATTTGAAATTACCTCCACCACGTCCAAAACCGATAGGTCGTCTTGTTCACCAACATCGTCTTGATTCGCCAAAGATTTCGATAACACCGGTTTGGGTTTACGATTTCAAACAACAACAACATTAACAATCGAGGGCGACATTCGATATCACGGTAATAAAATTCGAGTTTCACGTCGTGTTATGTTGATGGTTGTCTCAATGTATGATTTTGGTTCGATCTCACAATGTTAGTTATTATTTCGCATTAATTTTTGAAAACCTTGGAAAATTCAAAAACATTATGCATACTGAGATGTTTCTGTATTAACTTCTTCTTTGGTTGTTTATTGTTGCCGTATACAATGTTTCTGTATTAACTTCTTCTTTGGTTGTTTATTGTTGCCGTATACAATACATCTCTTACTTTCGATGTCGCCCTTTTGATTAATGGTTCTGATTTTGTTCATCTTTGAATTGTGGCAATATTATTCATCAGTTGTTGTAATATAATGTGTAGTTAGTTCAAAGATGGAAATAATGTGCATATAGATTACAAAGAGGTAATTGAAATTTGTGTAGGTTGCTGTATTGGAAATAATTAATCAATTTAGAACCTTCCTCATAGTGTAATGACACACCGTAAGGATACCCCTGTCGGTACCTTGGTTTGTTTGGCCACTATTGAAGAATGTTAGACCTTACATTTCTACTTTAGTGGAATGTTTAATTTTGGTAGACTTGAGAGATATTTATATTTCTCTACCAGTACCTTTAATGTGCTATCTATTTGTTTTATTTTTCAGTAATCAAATCTACCTATGTTAATGGTTGGGAAAAATGCATTTGTGGGTTYATTATTTGTATGCTTTTCTRTACTGTACGTGCCTTACATACTAANATGCACAYCTTGTCCAAATTATTTATTTATTTATTTTTCTTTCTTTGTAAACAATTCATAAATATTATAATTGG
TARGET=544,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05200 Ps_contig_mira-and-tgicl-ass_franssen_36784
SEQUENCE=CCTAAACCACTTGTCTNCNCTCTCGNRNCACAGCCAAACGCCCCACCCTTCTTCCCTCAACCGCGCCGTCGAAATCCCATCACCTCCGCCGTTCTCACGTGGGACCCTCACAAACACCTCCGTCTTCACGCACACCTCCGTGAAGTCTCAACCTCCCGACAACACAACCACAAACCGTCGGCTATTCTCCTTCCATTTCCTCCTTCAACCGCGTACCGCCGCCACAACCTCCAAACCCCCACCGCTCAAGCCACCACGCACAAACCTCAATAAAAATCCGGTGACGCCGCGTTTACAACCTCCTCCATTACATCGCCGCGCCGAATCCACCTCTCGCTAACCTTCAAGTTTCCGACCACCATAATTGTTTTCTTTAACTTCGTTCATTTATACAGGAAACAACAGTTTAACCACACACCTGTGCGGCCTCAGTTCATTTGAAATTACCTCCACCACGTCCAAAACCGATAGGTCGTCTTGTTCACCAACATCGTCTTGATTCGCCAAAGATTTCGATAACACCGGTTTGGGTTTACGATTTCAAACAACAACAACATTAACAATCGAGGGCGACATTCGATATCACGGTAATAAAATTCGAGTTTCACGTCGTGTTATGTTGATGGTTGTCTCAATGTATGATTTTGGTTCGATCTCACAATGTTAGTTATTATTTCGCATTAATTTTTGAAAACCTTGGAAAATTCAAAAACATTATGCATACTGAGATGTTTCTGTATTAACTTCTTCTTTGGTTGTTTATTGTTGCCGTATACAATGTTTCTGTATTAACTTCTTCTTTGGTTGTTTATTGTTGCCGTATACAATACATCTCTTACTTTCGATGTCGCCCTTTTGATTAATGGTTCTGATTTTGTTCATCTTTGAATTGTGGCAATATTATTCATCAGTTGTTGTAATATAATGTGTAGTTAGTTCAAAGATGGAAATAATGTGCATATAGATTACAAAGAGGTAATTGAAATTTGTGTAGGTTGCTGTATTGGAAATAATTAATCAATTTAGAACCTTCCTCATAGTGTAATGACACACCGTAAGGATACCCCTGTCGGTACCTTGGTTTGTTTGGCCACTATTGAAGAATGTTAGACCTTACATTTCTACTTTAGTGGAATGTTTAATTTTGGTAGACTTGAGAGATATTTATATTTCTCTACCAGTACCTTTAATGTGCTATCTATTTGTTTTATTTTTCAGTAATCAAATCTACCTATGTTAATGGTTGGGAAAAATGCATTTGTGGGTTYATTATTTGTATGCTTTTCTRTACTGTACGTGCCTTACATACTAANATGCACAYCTTGTCCAAATTATTTATTTATTTATTTTTCTTTCTTTGTAAACAATTCATAAATATTATAATTGG
TARGET=1332,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05201 Ps_contig_mira-and-tgicl-ass_franssen_36786
SEQUENCE=AGTACTCGGGGATGCCAAACCCAAACCAGGTTCTATATTCTAAGGAGTAAGCATAGAAAAACGCAATGGAATTGCACTTACTCATATTACTATTATTGTTATTGTTATAATCTTCTTGAATTTGTTTATTTCTTGCTTCACTTTGCTACAACCGTCGCTAAATATCATGGCGAAGGGTCGCAAATTCAGCGCGAATCGGAACGAGCGTTTGTTAGGAACGAACTATATCCAAAGCTCGTCTGTTAGTCATGAAGTGACCGATTTCAGGGAAGAAGACGTGTGGTCCGTGGTGGACGATCGTGATCGTGACCGTGATGTGGATTTCTCTCCCGGCGAGTGGGATTCACGCGCTTCTTCGTGGAGTCGGGAGAGAGAGAAGGAACACCGGAAGTTTGGTGGTTTGTCGCAGGCGTTTGAGGATTCCGGGAGTAATGTTGCGACGACGACTACGTCGAGGATCGTGCACCACCAGTACCGTGCTTCGCTGGGACGTAACGTGGCCACGTCAGCGCCGGTGAACGTGCCTGACTGGAGTAAGATACTCCGGGTTGAGTCGGTGGAATCGTTGCATGATATGGATGATGGTTTTGATGAGGACGAATCGGAGATGGTTCCGCCGCACGAGTATTTGGCGCGTGGCCGGAAAATGGCGGCAAACTCGGTTTTCGAAGGCGTGGGTCGGACTTTAAAGGGGCGTGACTTGAGACGGGTTCGTGATGCTGTTTGGAACCAAACCGGGTTCGATGGTTGAGATGGACCGGTTTAATATAATTCTATTAAACCGGATTAATTCTTGTTTGTTTGCTGATGTAATGTCATGCTTATTATATGTAATAAGAATACACAAAATTTAGATTCTTGATGTAACTTGGAACAATTTTTGTAACGGATTATTTGTTGATCCTGTATGACAAAACAGATGATCGCGTTTAAAGGACTAGGCCACTAGGAGCGAACCAATACATCGACACTTCTTTGCATTATACTCAACTAACAATAAGCATCTCAATGTGTCTTTCGCGTTCCCACAAAGTAGTTTTAGATGCCGAGAAAAGTATGGCAGATTATTTTATGACTCATGTTAGTTCCTACTTGGCAGCAGAAGCTTCGTCGAGCAAAGCAAAATTGATCAGATCCTCACTTTTGCTTCCTTGGAACTTCTGATTCTCTCTCTAATGCCATGGATGCTCTCGAGGAGTTCCGTTGTTTGTTGCCACCTGAAATTCTTTGGTCTCTCTTCACTATCCAATACTAAAATCGAGACATTCAAAACAGAAACTAGTTCAGTTTCAATCTCACTAAGCTTTTGATCTATATGTTTCATGAAGTCCTCGTAAGAGGATATTTTCGACAAGCTGCTGGGTATGCTGTTTTCTTTCCCAGCATCAGTGCTAAGGGTTTTCAGAAGATTATCTATCTCCTTATCGTCCAGTGGATTCTCTCGACTTTCGAAAGAAATTTGCACATCTTGAATATCGTTGAACTTCATCTTATTAGAGTCAGAATCATCGTTGAGAGAATCTTCTAGAACTTCCTCCTTTGGAGCAATGATTCGAACTGGACTCGAATCATCTTGTGTCGCATCTTCAATCTTCATAGTCACATTTCCAACTAGCTGGCTTTCTTTCAAAGATATTGCCTCACTTAGCTTTGCGGAAAGCTCGTCAAGCAACTCAATGCGGATTTTAGATTTTTCCAAAGCCTGCAACACTTGTTTCCTCTGAAGAAGCGTGTCCCTTGCATAATCTTCATCCCCCTTCTGGATACTTATAGCTGCTTGTTTTTGAAGCTTCTCAGCTGATTCTGATAATCTCAATAGCCTCAATCTAGCACTATTTGCTGTATTTAACCACCACACATAAGTTTTCATATCTTTTAACTAAACTCTTAGCTTTACTTTGGACATGCTTGCATTCTAGCAAAAGGTACAAAAACCTTTGGTTCTTGTGG
TARGET=368,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5932
PRIMER_LEFT_PENALTY=0.052187
PRIMER_RIGHT_PENALTY=0.541030
PRIMER_LEFT_SEQUENCE=TCCAAAGCTCGTCTGTTAGTCA
PRIMER_RIGHT_SEQUENCE=ATTACTCCCGGAATCCTCAAAC
PRIMER_LEFT=227,22
PRIMER_RIGHT=432,22
PRIMER_LEFT_TM=60.052
PRIMER_RIGHT_TM=60.541
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.7123
PRIMER_LEFT_1_PENALTY=0.171245
PRIMER_RIGHT_1_PENALTY=0.541030
PRIMER_LEFT_1_SEQUENCE=CTAAATATCATGGCGAAGGGTC
PRIMER_RIGHT_1_SEQUENCE=ATTACTCCCGGAATCCTCAAAC
PRIMER_LEFT_1=157,22
PRIMER_RIGHT_1=432,22
PRIMER_LEFT_1_TM=59.829
PRIMER_RIGHT_1_TM=60.541
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.7311
PRIMER_LEFT_2_PENALTY=0.190078
PRIMER_RIGHT_2_PENALTY=0.541030
PRIMER_LEFT_2_SEQUENCE=TTGCTACAACCGTCGCTAAATA
PRIMER_RIGHT_2_SEQUENCE=ATTACTCCCGGAATCCTCAAAC
PRIMER_LEFT_2=142,22
PRIMER_RIGHT_2=432,22
PRIMER_LEFT_2_TM=59.810
PRIMER_RIGHT_2_TM=60.541
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.9435
PRIMER_LEFT_3_PENALTY=0.402462
PRIMER_RIGHT_3_PENALTY=0.541030
PRIMER_LEFT_3_SEQUENCE=GTCATGAAGTGACCGATTTCAG
PRIMER_RIGHT_3_SEQUENCE=ATTACTCCCGGAATCCTCAAAC
PRIMER_LEFT_3=245,22
PRIMER_RIGHT_3=432,22
PRIMER_LEFT_3_TM=59.598
PRIMER_RIGHT_3_TM=60.541
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=1.0011
PRIMER_LEFT_4_PENALTY=0.402462
PRIMER_RIGHT_4_PENALTY=0.598673
PRIMER_LEFT_4_SEQUENCE=GTCATGAAGTGACCGATTTCAG
PRIMER_RIGHT_4_SEQUENCE=TATCTTACTCCAGTCAGGCACG
PRIMER_LEFT_4=245,22
PRIMER_RIGHT_4=540,22
PRIMER_LEFT_4_TM=59.598
PRIMER_RIGHT_4_TM=59.401
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_05202 Ps_contig_mira-and-tgicl-ass_franssen_36790
SEQUENCE=GGGGGGCTCACAAATCAAACCAACCTTTTTCTTTGGCGTGTGGTTTCTCTATTGCCCCGATCACCAAACTCGTTCGTTCGTTCGTTGTGAATGGTAGAAACCCTAAGCGATTTTGATTTCATGCGCTCTCGTTTCTCATTGAGGGCGAGCGCTATTCTCCTTCCAGTTTCTCCTTCATCTGCTTTCGCTTTCGGCTCTTGCAAACAAGGTTGGTACTACCGCTGCCTTGGCGTCGACCCCTGAGAAACTTCCCCCAAACCAAATAACCAGACGCGATTTTGTTTTCTGTTACTTCTTTTGTTTTAGAGATTCAGTTTTGGACTTTTAGGTTCTTTCATTGTTTGATTTCTCCGGATCGGTGATTTTATTTGTTGATCGAAAAGATGTTGATGAATAAGCATAAGAAAAACCAGAACGCCAATGCTAAGAGGCTTCTGATCAGTATCAATGTGTTTGGAAGCGCCGGACCGATCCGATTCGTTGTGAAGGAGGAGGAACTTGTTGCGGCAGTTATTGATACTGCTCTCAAGTCCTATGCTCGCGAAGGGAGACTCCCGGTTCTCGGAGGAGATATCAGAACTTTCGCTCTTTATTGCCCACTGGTCGGATCTGATGCTTTGAGTCCATGGGACACAATTGGATCAGTTGGAGCCAGAAATTTCATGCTGTGCAAGAAGCCACAAGCAACCACCGGAGATGAAGCTGCCAATGGGACTGGGACTGAAGTGGTTTCCCGGAGTAGAGGTGGAAGTTGGAAGGCTTGGTTCAACAAGTCTCTGAATTTGAAGATTTCTATCCATTGATCAATCAAGTTTTAAATTGTGTGCCAGCATTAAATAACTTATCATGTACTAGTACTCTGTTCTAGCTAGCAGAACTTTTGTAGAACTTTTGTTTTTTTGAGATTACTCTTCTTAGAGATTGTAGTTTTCAGTTTTGATCAATAAAAATTGAAATTTTGGTTTAGCTTTG
TARGET=70,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5071
PRIMER_LEFT_PENALTY=0.392624
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=GCTCACAAATCAAACCAACCTT
PRIMER_RIGHT_SEQUENCE=AAGCAGATGAAGGAGAAACTGG
PRIMER_LEFT=5,22
PRIMER_RIGHT=183,22
PRIMER_LEFT_TM=60.393
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.6478
PRIMER_LEFT_1_PENALTY=0.533373
PRIMER_RIGHT_1_PENALTY=0.114470
PRIMER_LEFT_1_SEQUENCE=CTTTGGCGTGTGGTTTCTCTAT
PRIMER_RIGHT_1_SEQUENCE=AAGCAGATGAAGGAGAAACTGG
PRIMER_LEFT_1=30,22
PRIMER_RIGHT_1=183,22
PRIMER_LEFT_1_TM=60.533
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=154
PRIMER_PAIR_PENALTY_2=0.7020
PRIMER_LEFT_2_PENALTY=0.392624
PRIMER_RIGHT_2_PENALTY=0.309418
PRIMER_LEFT_2_SEQUENCE=GCTCACAAATCAAACCAACCTT
PRIMER_RIGHT_2_SEQUENCE=GATGAAGGAGAAACTGGAAGGA
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=178,22
PRIMER_LEFT_2_TM=60.393
PRIMER_RIGHT_2_TM=59.691
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.7069
PRIMER_LEFT_3_PENALTY=0.392624
PRIMER_RIGHT_3_PENALTY=0.314269
PRIMER_LEFT_3_SEQUENCE=GCTCACAAATCAAACCAACCTT
PRIMER_RIGHT_3_SEQUENCE=GTTATTTGGTTTGGGGGAAGTT
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=266,22
PRIMER_LEFT_3_TM=60.393
PRIMER_RIGHT_3_TM=60.314
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.7458
PRIMER_LEFT_4_PENALTY=0.392624
PRIMER_RIGHT_4_PENALTY=0.353222
PRIMER_LEFT_4_SEQUENCE=GCTCACAAATCAAACCAACCTT
PRIMER_RIGHT_4_SEQUENCE=TGAAGGAGAAACTGGAAGGAGA
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=176,22
PRIMER_LEFT_4_TM=60.393
PRIMER_RIGHT_4_TM=60.353
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_05203 Ps_contig_mira-and-tgicl-ass_franssen_36798
SEQUENCE=GAAAATATATTTTAACAGTAGTGTAACACANTNCCAGAAACGGAGTTGCGAGTTGAAGCGATCGCCAACCATGGCGTCCTCACTATCACCTCTCAAGATAAACATGGAACTCCATTCCAAAAACCGACAGAACCTTCTCAAATCCCTTCGCCAACACCTCTCCAAATCCTCTCTTCCTCTTCACGGCTTCGTCCTCCTTCAAGGCGGGGAGGAACAAACTCGTTACGACACCGATCACGAAGAACTCTTCAGACAAGAGAGTTACTTCGCGTATTTGTTTGGAGTTAAAGAACCTGGTTTCTATGGAGCTATTGATGTTAGAAGTGGGGATTCTATCCTATTTGCTCCTAGGTTACCTGATGAGTATGCTATTTGGATGGGAAAGATCAAACCAGTTTCCCATTTTAAGGAACACTACAAGGTTACCACTGCCTATTTTACGGATGAGATTGTGACTGTTTTGCAACAAAATTACCAAGGTTCAGGGAAACCCCTGCTCTTTCTTTTGCATGGCCTTAACACAGATAGCAATAATTATGCTAAACCTGCGGAATTTCAGGGCATTGAGAAGTTTGATAAGGATCTGACGACTTTGCATCCTATATTGACTGAATGTCGTGTCATCAAGTCAGATCTGGAAATTGAACTTATGCAGTATGCCAATGATATAAGCTCTGAAGCTCATATTGAGGTTATGAGAAAAGCTAAAGCGGGCATGAAGGAGTATCAACTTGAAAGCATTTTTCTTCACCACACATACATGTATGGTGGATGTCGGCATTGCTCCTATACATGTATTTGTGCTACTGGTGATAATAGTGCGGTTCTACATTACGGCCATACAGCAGCTCCTAATGACAAGACTTTGGAAGATGGAGACATGGCATTGCTTGATATGGGAGCTGAATACCACTTCTATGGGTCTGACATAACATGTTCTTACCCGATAAATGGAAAGTTTACCAGTGACCAATCTCTTATATATAATGCTGTCCTTGATGCTCATGATGCTGTTATCTCTTCAATGAAACCTGGAGTAAGCTGGGTTGATATGCACATACTATCACATAAAGTGATTCTGGAGTCGCTAAAGAAGGGACACATTCTTCTGGGCAATGTTGATGATACTATGGTTGAACGCTTGGGTCATGTTTTCATGCCGCATGGTCTGGGGCATTTTCTTGGTTTAGATACTCATGACCCTGGAGGCTACCCAGAGGGCCTGGAAAGAAGAAAGGAACCTGGATTAAAAGCGTTGCGTGCGGCCCGAGGGCTCCAGGAAGGAATGGTTATCACGGTGGAGCCTGGATGCTACTTCATTGAAGCTTTGTTAATTCCAGCCATGAATAATCCAGAAACTTCCAAGTTCTTTAACAAGGAAGTGATTAACAGATTTACCGGCTTTGGTGGAGTGCGAATTGAGAGTGATCTGCTTGTTACTGCCACTGGTTGCTACAATATGACAAAGTGTCCCAGAAAAATACAGGAGATTGAAGCAGTGATGGCAGGGGCACCATGGCCGGTCAAGAAGACATCTACTACTGTTGAAAATGGATTGGAAAGCAAAGCCTGACTTCAAGCATCTTTAGACCTGAAAAGCTGTCATTTTCATTCTTTTTTTATTTATTTATATAAAAAACAGAGAAAATAACTCCCTCTCTACCCTACACCTTTTCAATTATTTTCCCTTTTGGGTGTACCTTTAAAATGAATATAGAAAAAGAAAAGCTTTAATGTAATCATAAGAAACAACGAAAATTGAAGTAATT
TARGET=1619,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0302
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.014945
PRIMER_LEFT_SEQUENCE=CGAATTGAGAGTGATCTGCTTG
PRIMER_RIGHT_SEQUENCE=AGGTACACCCAAAAGGGAAAAT
PRIMER_LEFT=1414,22
PRIMER_RIGHT=1701,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=59.985
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.0396
PRIMER_LEFT_1_PENALTY=0.024665
PRIMER_RIGHT_1_PENALTY=0.014945
PRIMER_LEFT_1_SEQUENCE=GCCACTGGTTGCTACAATATGA
PRIMER_RIGHT_1_SEQUENCE=AGGTACACCCAAAAGGGAAAAT
PRIMER_LEFT_1=1441,22
PRIMER_RIGHT_1=1701,22
PRIMER_LEFT_1_TM=60.025
PRIMER_RIGHT_1_TM=59.985
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.0724
PRIMER_LEFT_2_PENALTY=0.057411
PRIMER_RIGHT_2_PENALTY=0.014945
PRIMER_LEFT_2_SEQUENCE=GATCTGCTTGTTACTGCCACTG
PRIMER_RIGHT_2_SEQUENCE=AGGTACACCCAAAAGGGAAAAT
PRIMER_LEFT_2=1426,22
PRIMER_RIGHT_2=1701,22
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=59.985
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.0724
PRIMER_LEFT_3_PENALTY=0.057411
PRIMER_RIGHT_3_PENALTY=0.014945
PRIMER_LEFT_3_SEQUENCE=TGATCTGCTTGTTACTGCCACT
PRIMER_RIGHT_3_SEQUENCE=AGGTACACCCAAAAGGGAAAAT
PRIMER_LEFT_3=1425,22
PRIMER_RIGHT_3=1701,22
PRIMER_LEFT_3_TM=59.943
PRIMER_RIGHT_3_TM=59.985
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.0724
PRIMER_LEFT_4_PENALTY=0.057411
PRIMER_RIGHT_4_PENALTY=0.014945
PRIMER_LEFT_4_SEQUENCE=AGTGATCTGCTTGTTACTGCCA
PRIMER_RIGHT_4_SEQUENCE=AGGTACACCCAAAAGGGAAAAT
PRIMER_LEFT_4=1423,22
PRIMER_RIGHT_4=1701,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=59.985
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=279
=
PRIMER_SEQUENCE_ID=SSR_05204 Ps_contig_mira-and-tgicl-ass_franssen_36799
SEQUENCE=GGGGGCGTCANYGRGGCAAAGAAGGCACGAGAAGAAGAGATACATATCATCCATCACGCGCAAAATACGTGACTGCTAGATCTAGAGAGTAAAATCTTGCGGTGGCGTAGCAAAAGAGAGAGAGAATCAAACCATAGATCATGAGAACTCGCCGCCCGCACACTCACTCCGCCGCCGCAACCGCAATTCCATCCTCTCTTCTTCTAATTCTAACAACAACATGTCTCCTTCTTCTCTCTTTTTCAATTCCCACTCAATCCGAAGTCATCACTTTAACCTCCGACACCTTTTCCGATAAGATAAAGGAGAAAGATACTGCGTGGTTTGTGAAATTCTGCGTTCCCTGGTGCAAGCATTGCAAGAATTTGGGCTCGTTATGGGATGATATAGGGAAGGCAATGGAAAATGAAAATGAAATAGAGATTGGAGAAGTTGATTGTGGCACGGATAAAGCTGTTTGTTCTAAAGCTGATATTCACTCATATCCTACGTTTAAGGTTTTCTATGATGGAGAAGAAGTTGCCAAATATCAAGGTACAAGGGATGTTGAATCATTGAGAACTTTTGTTTTGGATGAAGCTGAAAAGGCAGCAGCAAAAGCACAACTCGACAGTGATAAAGAATTGTAACTAGTTTTGAGGTAGCAACCGAGCCTCCCACTCCCTTCACAATTATTCTTTTATTCTCTCTAGAATTTTGTGATAGTATGTTGTAAGGGATGACTTAAAATCTCAGACGACGGTGTTAAATCAGTGTGTGAGGCCATTTTTCTTTTTTGTTTCACATTTGATATTGATTCTAGGAAAGTTCTGTGGAGGATATCTTAATTCTTGTGAACAAATTTCCCATTTAGGATGAATTACCTGAAAAGAATGGTTAGTTCAGTCAACATTAACAAGTCTGCTATATCGGTTAATAATCTAAATTTGAAATAATACTTGCCACCAATAAAATT
TARGET=115,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05205 Ps_contig_mira-and-tgicl-ass_franssen_36801
SEQUENCE=TGTTTAAGGTTTGATGATTCTGGGTTTGGTTCTTTAGGGTTTTGATAAAAATGGGGTTAGGTGGTGAAAATGCTAAGGTAGTAGTAGAGTCTTTGGATGTGTGTAAATCAAAAGGGAGAAAGAAGAAGAAGAAGCAAGATGGGGTAATGGAAGATGAAGAAACTGAAACTGGGTGTTGGCTTAGCTTTAGGTTCATTGGAAGCTGCATTTCTTCAAGATCCAAAGTTGATAGCTCTGTTAGTGGCGCAAGTACTAATTATGCTGAAAGCAAATCAACTATTGATACAAGTAGAGACCAGCCATCGGTTCCAGTAGCCTCTTCGACAACCACTACTACTAGTAACGCTGAAAGCAACTCATCTACTTCGAAACTTGAAGAGGAGCTTAAAGTTGCTTCCAGGCTTCGAAAATTCTCTTTCAACGATCTTAAGTTGGCAACAAGAAATTTTCGGCCCGAGAGTCTTCTTGGTGAAGGTGGGTTTGGTTGTGTGTTCAAAGGATGGATCGAAGAAAATGGAACTGCTGCTGTGAAACCTGGTACTGGACTTACCGTTGCTGTCAAAACCCTTAACCACGATGGTCTCCAAGGTCATAAAGAATGGCTGGCTGAAGTAAATTATCTCGGTGATTTGGTTCATCAAAACCTTGTCAAACTTATAGGCTACTGTATCGAGGATGATCAAAGGTTGCTTGTATACGAAGTTTAATGCCTCGTGGAAGCCTGGAAAACCACTTGTTT
TARGET=120,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1939
PRIMER_LEFT_PENALTY=0.056343
PRIMER_RIGHT_PENALTY=0.137550
PRIMER_LEFT_SEQUENCE=TGATAAAAATGGGGTTAGGTGG
PRIMER_RIGHT_SEQUENCE=GGATCTTGAAGAAATGCAGCTT
PRIMER_LEFT=42,22
PRIMER_RIGHT=220,22
PRIMER_LEFT_TM=59.944
PRIMER_RIGHT_TM=59.862
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.1939
PRIMER_LEFT_1_PENALTY=0.056343
PRIMER_RIGHT_1_PENALTY=0.137550
PRIMER_LEFT_1_SEQUENCE=TGATAAAAATGGGGTTAGGTGG
PRIMER_RIGHT_1_SEQUENCE=ATCTTGAAGAAATGCAGCTTCC
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=218,22
PRIMER_LEFT_1_TM=59.944
PRIMER_RIGHT_1_TM=59.862
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.2331
PRIMER_LEFT_2_PENALTY=0.095574
PRIMER_RIGHT_2_PENALTY=0.137550
PRIMER_LEFT_2_SEQUENCE=GTTTGATGATTCTGGGTTTGGT
PRIMER_RIGHT_2_SEQUENCE=ATCTTGAAGAAATGCAGCTTCC
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=218,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=59.862
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.2331
PRIMER_LEFT_3_PENALTY=0.095574
PRIMER_RIGHT_3_PENALTY=0.137550
PRIMER_LEFT_3_SEQUENCE=GTTTGATGATTCTGGGTTTGGT
PRIMER_RIGHT_3_SEQUENCE=GGATCTTGAAGAAATGCAGCTT
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=220,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.862
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=0.2384
PRIMER_LEFT_4_PENALTY=0.056343
PRIMER_RIGHT_4_PENALTY=0.182061
PRIMER_LEFT_4_SEQUENCE=TGATAAAAATGGGGTTAGGTGG
PRIMER_RIGHT_4_SEQUENCE=TAAAGCTAAGCCAACACCCAGT
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=188,22
PRIMER_LEFT_4_TM=59.944
PRIMER_RIGHT_4_TM=60.182
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_05206 Ps_contig_mira-and-tgicl-ass_franssen_36804
SEQUENCE=GAAAACTCTTCTCTTTTCTTCTTTCACTTTCTTCCAAACACAAGCTCAAATGGTGTTACTATCCAAACCAACTTCAGAACAATACACCTATGTTAGGAACAACATGCCAATCACATTTTCTTCATCAATCCCTCTCGTTGACCTATCAAAACCAGATGCAAAAACCCTCATAGTAAAAGCCTGTGAAGATTTTGGATTCTTCAAAGTTATAAACCATGGTATCCCTTTGGATGCTATCTCCCAATTGGAATCCGAGGCCTTCAAATTCTTCTCTCTCCCTCAAACAGAGAAAGAAAAAGCAGGGCCTGCAAATCCTTTTGGCTATGGTAACAAACGTATTGGACTCAATGGTGATATTGGTTGGATTGAATATCTTCTCCTCACAACCAATCAAGAACACAATTTCTCTCTTTATGGAGAAGACATAGACAAATTTAGGGGTTTGTTGAAAGATTATAAGTGTGCAATGAGGAATATGGCATGTGAGATACTTGATTTGATGGCAGAAGGGTTAAAGATACAACCAAAGAATGTGTTTAGCAAGCTTGTGATGGATAAACAGAGTGACTGTCTTTTTAGGGTTAATCATTACCCTGCTTGCCCTGAATTAGCTATCAATGGTGAGAATTTGATTGGGTTTGGAGAACACACTGACCCTCAAATTATTTCAATTTTGAGGTCAAATAATACTTCAGGCTTTCAAATTTCTCTTAGAGATGGTAGCTGGATTTCAGTTCCTCCTGATCATAGCTCCTTCTTTATCAATGTTGGTGATTCTCTTCAGGTTATGACTAATGGGAGGTTCAAAAGTGTGAGACATAGAGTTTTGGCAAATGGCATAGACCCAAGGCTGTCTATGATTTACTTTTGTGGACCACCTTTGAGTGAGAAAATAGCACCATTACCTTCACTCATGAAAGGAAAAGAAAGTTTGTATAAAGAATTTACATGGTTTGAGTACAAGAGTTCAACTTATGGTTCAAGGTTGGCTGATAATAGGCTTGGAAATTATGAAAGGATTGCTGCCACTTAATATTGGAGGATCATAGTGTTGTTCAATTGTCAATAGAAATGGGGATACAATATATATCTATAATTCAATCAAAATCAACTTCAAAATACAATGTTTGTTAAGATAGAGTTGTATGTAGTATAACTATGATAAGTTTTTTTTTATGTATTTTTCTTTATGGTACTTTAAGGTTACATTTATTAATTATGAGGTGTCATATAGATATGATAGTATTTATTCCCCTTTTGCATCAACACTCATGTAAGTACTTTTCTAAGTATATGAAATATTTCATATGTTTGTGTTAAATATGTACCATGTGATTGATTTGGGTCTAGTATTTTTCTAATAATTTTTTTTAGTGGTTCTTTTAAGTTTTTGTTAATTGATTATTATGTTATGTTATGCTTCTATTGCACTATTTCTCACAAATAAAATGTATGACATTTTAATATCTATTAATCACAATTTATATTAAAGATAAATAATCTATTATTACGAGTTTGTGAATTCGGATTATTAAGTATAACTTTTAAATTTATAAAAAAATATTAGTGCCAAATCTAACATGTTTTTGTGAATAATATTAACAATTTTTTAAACAAAATTTTATACATAAAAAGACTAACTTACGTATGCATAATTTACATTAACTCTNAATTAGTGGATATATAA
TARGET=1405,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.9287
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=4.891399
PRIMER_LEFT_SEQUENCE=CCTTTTGCATCAACACTCATGT
PRIMER_RIGHT_SEQUENCE=ATCCGAATTCACAAACTCGT
PRIMER_LEFT=1253,22
PRIMER_RIGHT=1528,20
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=57.109
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=5.2064
PRIMER_LEFT_1_PENALTY=0.314970
PRIMER_RIGHT_1_PENALTY=4.891399
PRIMER_LEFT_1_SEQUENCE=CCATGTGATTGATTTGGGTCTA
PRIMER_RIGHT_1_SEQUENCE=ATCCGAATTCACAAACTCGT
PRIMER_LEFT_1=1327,22
PRIMER_RIGHT_1=1528,20
PRIMER_LEFT_1_TM=59.685
PRIMER_RIGHT_1_TM=57.109
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=5.3403
PRIMER_LEFT_2_PENALTY=0.448874
PRIMER_RIGHT_2_PENALTY=4.891399
PRIMER_LEFT_2_SEQUENCE=TACCATGTGATTGATTTGGGTC
PRIMER_RIGHT_2_SEQUENCE=ATCCGAATTCACAAACTCGT
PRIMER_LEFT_2=1325,22
PRIMER_RIGHT_2=1528,20
PRIMER_LEFT_2_TM=59.551
PRIMER_RIGHT_2_TM=57.109
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=204
PRIMER_PAIR_PENALTY_3=5.3758
PRIMER_LEFT_3_PENALTY=0.484361
PRIMER_RIGHT_3_PENALTY=4.891399
PRIMER_LEFT_3_SEQUENCE=ACCATGTGATTGATTTGGGTCT
PRIMER_RIGHT_3_SEQUENCE=ATCCGAATTCACAAACTCGT
PRIMER_LEFT_3=1326,22
PRIMER_RIGHT_3=1528,20
PRIMER_LEFT_3_TM=60.484
PRIMER_RIGHT_3_TM=57.109
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=5.9060
PRIMER_LEFT_4_PENALTY=1.014618
PRIMER_RIGHT_4_PENALTY=4.891399
PRIMER_LEFT_4_SEQUENCE=CCCTTTTGCATCAACACTCAT
PRIMER_RIGHT_4_SEQUENCE=ATCCGAATTCACAAACTCGT
PRIMER_LEFT_4=1252,21
PRIMER_RIGHT_4=1528,20
PRIMER_LEFT_4_TM=59.985
PRIMER_RIGHT_4_TM=57.109
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=277
=
PRIMER_SEQUENCE_ID=SSR_05207 Ps_contig_mira-and-tgicl-ass_franssen_36808
SEQUENCE=AGGGATGTAATGCTGATGNTAATGGATGGCCGTGATTGTGTATGGAAATAATTCGGATGGAGCTGCTGCTGCTCCCCGTGATGCAAATTTGATGGCCGATTAGACGTTAACATAGCATTCAAGTTGTACGGAGGTGGTCGATGAGGTATAACCAAACCCACCTGAAGTTTATTCGACCCGGTTTCTGAATCTGATTTTCCACGACCGTAAAGAGGGACTAATGAAGTATGCGAGATGACAGCTTTACAAACAGGGCATGTAGGCTGCTTATCTGGTTCAACGGAAGAGTTTTGGACATTAAGCCATTTATATATACATGGCCAGCAATAGAGATGACCACAAAGTGTAACCACAAGATCATTTGCTGATTCCAAGCAAATATTACAATCAAAGCAACCATTTTCACCACTAGAGATTGTTCTCTCTTCTTCTTCTTCTGTAGTAGATTTACATTTTTGCTTTAGTGAGACTTTCACATTAGAACCAAAGCATGTAGATTCCATTGAATTTTCTACTACRGAAATATACCCTTTGGAAATAAAGAAATTAAGACGTTAGCTTAGCTTGAAAGGAAGAGAAAAGGAAAAAAGTAGAAAGGTGGGAGAGAATTGGATTGAGAAGAGCGCAATGGAGGARAGTAAATTGAGGATGAAGAAGAAGAAGATTGTGTTGTAAGGAAACCGAAC
TARGET=62,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05208 Ps_contig_mira-and-tgicl-ass_franssen_36808
SEQUENCE=AGGGATGTAATGCTGATGNTAATGGATGGCCGTGATTGTGTATGGAAATAATTCGGATGGAGCTGCTGCTGCTCCCCGTGATGCAAATTTGATGGCCGATTAGACGTTAACATAGCATTCAAGTTGTACGGAGGTGGTCGATGAGGTATAACCAAACCCACCTGAAGTTTATTCGACCCGGTTTCTGAATCTGATTTTCCACGACCGTAAAGAGGGACTAATGAAGTATGCGAGATGACAGCTTTACAAACAGGGCATGTAGGCTGCTTATCTGGTTCAACGGAAGAGTTTTGGACATTAAGCCATTTATATATACATGGCCAGCAATAGAGATGACCACAAAGTGTAACCACAAGATCATTTGCTGATTCCAAGCAAATATTACAATCAAAGCAACCATTTTCACCACTAGAGATTGTTCTCTCTTCTTCTTCTTCTGTAGTAGATTTACATTTTTGCTTTAGTGAGACTTTCACATTAGAACCAAAGCATGTAGATTCCATTGAATTTTCTACTACRGAAATATACCCTTTGGAAATAAAGAAATTAAGACGTTAGCTTAGCTTGAAAGGAAGAGAAAAGGAAAAAAGTAGAAAGGTGGGAGAGAATTGGATTGAGAAGAGCGCAATGGAGGARAGTAAATTGAGGATGAAGAAGAAGAAGATTGTGTTGTAAGGAAACCGAAC
TARGET=424,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05209 Ps_contig_mira-and-tgicl-ass_franssen_36808
SEQUENCE=AGGGATGTAATGCTGATGNTAATGGATGGCCGTGATTGTGTATGGAAATAATTCGGATGGAGCTGCTGCTGCTCCCCGTGATGCAAATTTGATGGCCGATTAGACGTTAACATAGCATTCAAGTTGTACGGAGGTGGTCGATGAGGTATAACCAAACCCACCTGAAGTTTATTCGACCCGGTTTCTGAATCTGATTTTCCACGACCGTAAAGAGGGACTAATGAAGTATGCGAGATGACAGCTTTACAAACAGGGCATGTAGGCTGCTTATCTGGTTCAACGGAAGAGTTTTGGACATTAAGCCATTTATATATACATGGCCAGCAATAGAGATGACCACAAAGTGTAACCACAAGATCATTTGCTGATTCCAAGCAAATATTACAATCAAAGCAACCATTTTCACCACTAGAGATTGTTCTCTCTTCTTCTTCTTCTGTAGTAGATTTACATTTTTGCTTTAGTGAGACTTTCACATTAGAACCAAAGCATGTAGATTCCATTGAATTTTCTACTACRGAAATATACCCTTTGGAAATAAAGAAATTAAGACGTTAGCTTAGCTTGAAAGGAAGAGAAAAGGAAAAAAGTAGAAAGGTGGGAGAGAATTGGATTGAGAAGAGCGCAATGGAGGARAGTAAATTGAGGATGAAGAAGAAGAAGATTGTGTTGTAAGGAAACCGAAC
TARGET=651,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05210 Ps_contig_mira-and-tgicl-ass_franssen_36810
SEQUENCE=TAGTTTTGTTATAATTACTATTAACTAATTAATAGGATNANCTAATTTAATTTAGTTACTTAACGTATACTGCACTAAGACCACTACTATATTGTACAGCAATAACAATCAAGTCTCATTCTTCTAAATAATAGATCAAATTTTGTTATAATGTTCTGTCCACTTCACACTATGTCATAGAGATTCAAGAGAGTTTTACATGGTAAACTAATCAAAAATTGACTTTCAAGGCCAACTAAAAAGTCTGCTTGTATTCTGGGTGATGGTTTAGACTGGTCGTTAACAATTTACTAATTATCCTTTGAAGCCAGGGCAGTTTTTGATCCGTACTTGGAGAAAACACCACTCAATGCAGCCTTTTCCTTGATCTTTAACCTTTTCGCAGATACAGGAACATCTAAAACATTTTTTGCATTCTCTTTTGTACTTGGTTTTTGCTTAGGGGCATTCTTTTGTCCAACTTTGACAGATGCTTTCTTCTTTCCAACAACCTGCTTTGAGGGAAGTGAACTGATTTCAGAATTATTTGCCATTTCTACTCTGTCATCACTATCCAAATTCAAAGCAGTGACAAACTCTTCATTCTCCCCATCACTTTCCCTTTGATCATCTGCATTATCTAAATCAGTTTCTTCCCAGGTACCTCGGGCAGAATCGTTCATGTCAAAATCCTCATTTGAATCTTCTTCGAAGGAGGGCAAGAGAGGTAGAGTAGTGTTGAGATTTTTTCTTAATTTCAGAGAAGAAGAGGAAGACCGTGCATTTACAAGCTCGCATTTTATCTGCTTCTGAATTTTGCCAAAAGTAATAGTTTGTCCGGTGTATAACTCTACAAACCTAACTGCATCTTCTTCATGCTTGAAATCAACAAAAGCAAAACCCTTACAGATAGGATCCCTTGTCTTCTTGTTTCCAATAACAGCAGGGACTATATTTACTATACCCGGAATCCCTTGAAAAGCTGATTTCAAATCCCTATCAATTTTCCTTTTCTTTGGTAGATTCACCAAACGCACTTGGCTTCCGATAGGAACAGCCTTTTCATCTATCTTCTTCTGAGCATCATTTTTGGAGGCAGTGTTGATAGGGTTTGTTTTAAGCCTTTTCAAATTAGCAGCTTGTGCTCGTTTACTTTGCTGCAATTCTTCAAGCATCTTATCTTCAATTGAAGTGTCATACTCTTTCCCTTCACCAAACCCCTTCGGCTTCTTTTCAAACCACTCTCTCATCTCATCAGACGCAACTATCTCTTCTTCCACATCATCATCATCATCATCATCATCAGCAACCTCTTCGTCGTCCTCTTCATCGTATTCGAAACTAACATCCTCGTCATTTTCGTCGTCGAAATCCTCCTCAATTTCAACTAGGTTCACGCTTCCACTTCCTCGCTTCTTCGCAGCCTCCGGGTCGAATCCAGCACCACCGCGTACGGCGCGCAGACACTTCACGCGGAGTTGCCGGAATTGTAGGAGACCGAATCTCGAAACGAAAGAAGAACGAGGATAAATAACACGTGTGGTTTGAAGATAACGACACGTGTTGTTTACAGAGGTAACCGCCACGGAATTGTTCGGTGCCGGAAATGCAGTTGCTCGGAGCATTATTTTATTTGTTAGTCACAGCGATCGTGTAAACTTACAACCC
TARGET=1259,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0747
PRIMER_LEFT_PENALTY=0.019767
PRIMER_RIGHT_PENALTY=0.054900
PRIMER_LEFT_SEQUENCE=TCTCATCTCATCAGACGCAACT
PRIMER_RIGHT_SEQUENCE=GTTGAAATTGAGGAGGATTTCG
PRIMER_LEFT=1223,22
PRIMER_RIGHT=1366,22
PRIMER_LEFT_TM=60.020
PRIMER_RIGHT_TM=59.945
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=0.0747
PRIMER_LEFT_1_PENALTY=0.019767
PRIMER_RIGHT_1_PENALTY=0.054900
PRIMER_LEFT_1_SEQUENCE=CTCTCATCTCATCAGACGCAAC
PRIMER_RIGHT_1_SEQUENCE=GTTGAAATTGAGGAGGATTTCG
PRIMER_LEFT_1=1222,22
PRIMER_RIGHT_1=1366,22
PRIMER_LEFT_1_TM=60.020
PRIMER_RIGHT_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=0.1153
PRIMER_LEFT_2_PENALTY=0.019767
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=CTCTCATCTCATCAGACGCAAC
PRIMER_RIGHT_2_SEQUENCE=TCGAGATTCGGTCTCCTACAAT
PRIMER_LEFT_2=1222,22
PRIMER_RIGHT_2=1486,22
PRIMER_LEFT_2_TM=60.020
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.1153
PRIMER_LEFT_3_PENALTY=0.019767
PRIMER_RIGHT_3_PENALTY=0.095574
PRIMER_LEFT_3_SEQUENCE=TCTCATCTCATCAGACGCAACT
PRIMER_RIGHT_3_SEQUENCE=TCGAGATTCGGTCTCCTACAAT
PRIMER_LEFT_3=1223,22
PRIMER_RIGHT_3=1486,22
PRIMER_LEFT_3_TM=60.020
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=0.1266
PRIMER_LEFT_4_PENALTY=0.071684
PRIMER_RIGHT_4_PENALTY=0.054900
PRIMER_LEFT_4_SEQUENCE=AGGCAGTGTTGATAGGGTTTGT
PRIMER_RIGHT_4_SEQUENCE=GTTGAAATTGAGGAGGATTTCG
PRIMER_LEFT_4=1072,22
PRIMER_RIGHT_4=1366,22
PRIMER_LEFT_4_TM=59.928
PRIMER_RIGHT_4_TM=59.945
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_05211 Ps_contig_mira-and-tgicl-ass_franssen_36814
SEQUENCE=GGATTCAAATGTCCGGTACTTGACAAGTCACGGCTGGGCTGTGTTGTGTTGTGTTGCTACAGAGATGCTGACGGCGCTTGCTCCTTATCCTTCCTCCACATTCCGACCAACATCCATTCGCTGCGCCAACGACCGTCAGGCTCTGTTTAACCGCATCGCTCCTGTCTATGATAACCTGAATGATTTACTGAGCTTGGGCCAGCACCGGATTTGGAAGCGAATGGCAGTTTCCTGGACTGGAGCAAAAATGGGGGACCGTGTGTTGGATGTATGCTGTGGAAGTGGTGATTTAGCATTTCTCTTGTCCAACAATGTTGCCTCCCATGGCAAGGTGATTGGTCTTGATTTCTCAAAGGAACAGCTAACGGTTGCCTCATCACGGTCGTTTTCAAAGAACTGCCACAAGAATATCGAGTGGGTTCAAGGTGATGCTCTTGACTTGCCATTTGATGATGGTTGGTTTGATGCAATCACTATGGGATATGGTCTTCGAAATGTTGTTGATAAGCGTAAAGCCATGCAGGAAATATTACGTGTACTGAAGCCCGGTTCTACAGTGTCTATCCTTGACTTCAATAAAAGCAACGAGCTGCTAACTTCTTCATTTACGGAATGGATGATTGACAATGTTGTTGTTCCTGTAGCCTCTGTTTATGGCCTTTCAGAAGACTACAAATATCTAAAGAGTTCCATAAGAGAGTTTTTAACAGGGAAGGAATTGGAGAAACTTGCTTTAGAAGTAGGATTTTCGACTGCCCGACATTATGAGATCGGTGGAGGACTCATGGGGTGCTTGGTAGCTAAACATTAGATGAGATTTAGGGATCATCTTTTTGTACCAATGCATTAAATAACAAACCAGAGCTCTTTTATCTCCTAACATTCTACTGTTAATTAATTATATTTACAAAGTTTGAAGGAAATGCTCTCCAATCATGTTTTCATCTTTA
TARGET=40,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05212 Ps_contig_mira-and-tgicl-ass_franssen_36816
SEQUENCE=AACAAGTAGGTAATCAACGCAGAGTACNCGGGGATCACACTCAGTTCTCATTTTCTTTGCATTTCTCAACTTGATTTCAAATATTCATGGAGTTACCTGTGAAACTCTTGTTTGCAGCATGTCTCATTCTTTCTGTCACCATTGATGGAACAAAGGCAGATACAATGCTCACTGGAACTGTCATCTGTGACCAATGCAAAGATGGCCAGAGATCTCTTTTTGATTATCCTGTTAATGGTGCAAAAGTGACTCTGTCATGTAGTGACAGCAATGGTGAAATAACAATGTCAAGAGAAGAAACAACAAATTGGTTTGGAAGCTATACAATGAGATTTGATGGTGCACCAGATTTATCTGGTTGTTCTGCACATGTCTCAGGAAGTGATAATGGACAAGGAGGATCGTCGATGAGTTGTAGTGAAGCTTCTGGTCCTGCTCAAAATCCTAGACTCGTGTTTAGGATGTTTGACATGGAAATGTATGTTGTTGATGCTCTTCTTGCTCAGCCTCCTCAACCTATGCAATACTGCTCAACAACTCCCAAACCAGCTCCAACACCATCTTTGCAGCCGCCACCAACATTCGCGCCGCGGATTTCTTCACCTCCTCATTTCAACCTACCACCGATGCCGCCACTTCCACCGCTACTTCCAATGCCGCAGCTTCCACCGTTACCTCCATTAGGTCCATTACCACCCGTGATCTTCGTAGGAGCTTCAGCATGTCCACCACAGACATGGTTAATGCCGGAATACGAATGCTACTGGAGGGGTGTGAACAGAGACACTAAAGTAGCAGTGGCTTTTGGAATGGTTGCAGCAAGGAGATATGGAACAGACATTACATTGTGGTATGGATTGAAAGGGAGAGGAGATCCTTATAGGACTCTTTTAAGAGAAGGGATAACTGCACTTCTTAACTCTTATAACAGCATTCAATTCCCTTATCATCCACTTGGTGTAATCCAACACATGAATTATGCTTTGATGGGCTCAACTAGGGATGTTCTTGTTACCGCTTTACACTTCATGAGGGCTAATTCTGGTGAAGCTGGAAATGTTACTTGCAAATTCACTTCTTGCAGTTAATTTATATATATATATGATATAGCCATTTTGCTAGCATGTTTGGGAAATGATTATTTTGAAAAGTTTACTTAGAAATTATTTTGAATAGAAAATATTGACTAATAAAATAGACCTAAACCCAA
TARGET=1091,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5150
PRIMER_LEFT_PENALTY=0.021195
PRIMER_RIGHT_PENALTY=1.493838
PRIMER_LEFT_SEQUENCE=TAGGGATGTTCTTGTTACCGCT
PRIMER_RIGHT_SEQUENCE=CCCAAACATGCTAGCAAAATG
PRIMER_LEFT=997,22
PRIMER_RIGHT=1131,21
PRIMER_LEFT_TM=60.021
PRIMER_RIGHT_TM=60.494
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=1.5150
PRIMER_LEFT_1_PENALTY=0.021195
PRIMER_RIGHT_1_PENALTY=1.493838
PRIMER_LEFT_1_SEQUENCE=CTAGGGATGTTCTTGTTACCGC
PRIMER_RIGHT_1_SEQUENCE=CCCAAACATGCTAGCAAAATG
PRIMER_LEFT_1=996,22
PRIMER_RIGHT_1=1131,21
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=60.494
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=1.6242
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=1.493838
PRIMER_LEFT_2_SEQUENCE=GGCTCAACTAGGGATGTTCTTG
PRIMER_RIGHT_2_SEQUENCE=CCCAAACATGCTAGCAAAATG
PRIMER_LEFT_2=989,22
PRIMER_RIGHT_2=1131,21
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=60.494
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=143
PRIMER_PAIR_PENALTY_3=1.6547
PRIMER_LEFT_3_PENALTY=0.021195
PRIMER_RIGHT_3_PENALTY=1.633469
PRIMER_LEFT_3_SEQUENCE=TAGGGATGTTCTTGTTACCGCT
PRIMER_RIGHT_3_SEQUENCE=CAAACATGCTAGCAAAATGGC
PRIMER_LEFT_3=997,22
PRIMER_RIGHT_3=1129,21
PRIMER_LEFT_3_TM=60.021
PRIMER_RIGHT_3_TM=60.633
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=133
PRIMER_PAIR_PENALTY_4=1.6547
PRIMER_LEFT_4_PENALTY=0.021195
PRIMER_RIGHT_4_PENALTY=1.633469
PRIMER_LEFT_4_SEQUENCE=CTAGGGATGTTCTTGTTACCGC
PRIMER_RIGHT_4_SEQUENCE=CAAACATGCTAGCAAAATGGC
PRIMER_LEFT_4=996,22
PRIMER_RIGHT_4=1129,21
PRIMER_LEFT_4_TM=60.021
PRIMER_RIGHT_4_TM=60.633
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=11.1000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_05213 Ps_contig_mira-and-tgicl-ass_franssen_36824
SEQUENCE=CTCTAGACCAATCACCACTCCATTTCTTTCNAACCAAACACACCCTAATACGTCACTTCATCGGCGTCCAGCCAACAAAACAAGCAACACACGATTTTCTGAATTTCCGACAGTTGACTTAAAGTTGGTTGAAATGTGGAGCCTTGGATTCATTGATTTGATTAGGGTTTCTATTTTGTTGGCTTTTGCGACTGTGATCTCTTCTCTGGAACCCCATCTGGGATCAACCCGCGTCGTTTTTCAGACAAATTACGGAGACATTGAATTTGGTTTCTATCCAACTGTTGCACCCAAAACTGTTGACCACATTTTTAAGCTTGTGAGACTTGGCGGCTATAACACCAATCACTTCTTTCGGGTGGATAAGGGTTTTGTAGCCCAAGTAGCTGATGTTGCAAATGGAAGATCTGCCCCCATGAATGAGGAACAAAGAAGAGTAGCTGAAAAGAACGTTGTTGGTGAATTTAGTGATGTCAAGCATGTTCGAGGCATTCTTTCCATGGGAAGGCATGACGATCCAGATAGTGGCGGATCCTCTTTTTCAATGTTACTTGGAGATTCTCCTCATCTTGATGGGAAGTATGCAATATTTGGAAAAGTTACAAAAGGCGACGATACATTGGCGAAGCTTGAGCAACTACCTACTCGTAAAGAGGGAATATTTGTAATGCCAACGGAGCGCATCACCATCCTCTCATCATATTACTATGATACGGAGATAGAAAATTGTGAGCAAGACAGATCGATTTTGAAGCTCAGGTTAGCTGCATCGGCTGTTGAAGTTGAAAGACAGAGAATGAAATGCTTCCCCTAATAGAAGCGAGCTGGGAATGGAATGGAATGGAGTTGGAATGAAAGTGTTTGAGTCAGTCAAGAGCAAAAATTGTTGACGCCTTACTTTTGTTTTTTGTATTTTTTTCCCGGTAGTTTTGTGTAATGTATTGGATTTTTTAAAAAAAATTGGTATAAAAGTATATCATTTATTTTTGATGTAATTATATCATTTAACTTTTACTTAATATGGATTTAACTT
TARGET=828,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1574
PRIMER_LEFT_PENALTY=0.076597
PRIMER_RIGHT_PENALTY=1.080765
PRIMER_LEFT_SEQUENCE=GCATCACCATCCTCTCATCATA
PRIMER_RIGHT_SEQUENCE=CATTACACAAAACTACCGGGAA
PRIMER_LEFT=680,22
PRIMER_RIGHT=938,22
PRIMER_LEFT_TM=59.923
PRIMER_RIGHT_TM=58.919
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=1.3076
PRIMER_LEFT_1_PENALTY=0.226866
PRIMER_RIGHT_1_PENALTY=1.080765
PRIMER_LEFT_1_SEQUENCE=ATATTTGTAATGCCAACGGAGC
PRIMER_RIGHT_1_SEQUENCE=CATTACACAAAACTACCGGGAA
PRIMER_LEFT_1=658,22
PRIMER_RIGHT_1=938,22
PRIMER_LEFT_1_TM=60.227
PRIMER_RIGHT_1_TM=58.919
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=1.4444
PRIMER_LEFT_2_PENALTY=0.020269
PRIMER_RIGHT_2_PENALTY=1.424124
PRIMER_LEFT_2_SEQUENCE=TACAAAAGGCGACGATACATTG
PRIMER_RIGHT_2_SEQUENCE=TTGCTCTTGACTGACTCAAACAC
PRIMER_LEFT_2=600,22
PRIMER_RIGHT_2=879,23
PRIMER_LEFT_2_TM=60.020
PRIMER_RIGHT_2_TM=59.576
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=1.5007
PRIMER_LEFT_3_PENALTY=0.076597
PRIMER_RIGHT_3_PENALTY=1.424124
PRIMER_LEFT_3_SEQUENCE=GCATCACCATCCTCTCATCATA
PRIMER_RIGHT_3_SEQUENCE=TTGCTCTTGACTGACTCAAACAC
PRIMER_LEFT_3=680,22
PRIMER_RIGHT_3=879,23
PRIMER_LEFT_3_TM=59.923
PRIMER_RIGHT_3_TM=59.576
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=1.5092
PRIMER_LEFT_4_PENALTY=0.428401
PRIMER_RIGHT_4_PENALTY=1.080765
PRIMER_LEFT_4_SEQUENCE=GAGAATGAAATGCTTCCCCTAA
PRIMER_RIGHT_4_SEQUENCE=CATTACACAAAACTACCGGGAA
PRIMER_LEFT_4=792,22
PRIMER_RIGHT_4=938,22
PRIMER_LEFT_4_TM=59.572
PRIMER_RIGHT_4_TM=58.919
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_05214 Ps_contig_mira-and-tgicl-ass_franssen_36828
SEQUENCE=CCAAACAAAACCCAATACAATAAACGATAGCTAGCTATCAACTCTCCCTCGTCGTCTCCGCCCCTCTCAACTGTCCTTCTTCATATCACCTCTCCTCTCATTCCATTCGATTTCACTCTCACTTTCCAAAATGAACGCACATTCCTCTTCGATAATCTCAATGGCTTCTCCTTCTCCTTCCCAATCTCTCCGCAACTCAACATCCCTCCCTTTCTCAACTCTCATCCTCCCCCGCAACTCCTCCCATCGCCGCCGTCACTCTTCCTTCAAAATCCAAGCCAAGATCCGCGAAATCTTCATGCCTGCACTCAGTTCCACCATGACCGAGGGTAAAATCGTCTCGTGGATCAAATCCGAAGGCGACACTCTCTCCAAAGGTGATAGTGTTGTTGTTGTTGAATCGGATAAAGCTGATATGGATGTTGAGACTTTCTATGATGGAATTCTTGCTGCTATTGTTGTTTCTGAAGGTGAAACTGCTCCGGTTGGTGCTCCGATTGGTTTGCTCGCTGAGACTGCTGAGGATATCGCGGAAGCTCAAGCGAAAGCCAAATCGGTCAAATCCGGTTCCGGTTCTTCTTCTCCTCCTCCTGAAATACCTGACACTCCTCCGGTGACTTCTCAGTCTCCTTCTCCTCCTCCGCCACCACCGGTTAAGGCTGTTTCTGATGGTCCTAAGAAAATCACGGCAACACCTCAGGCTAAGAAACTTGCGAAGCAGCACAAGGTTGACATTGCTTCCGTCAATGGGACTGGTCCGTTTGGTAGGATTACTCCGGCTGACGTGGAGGCTGCTGCTGGGATTGTGCCGTCGAAGAGTAATGCACCTTCTGTAGTAGCTTCTGCTCCGGTGGCATCGGCTCCACCAAAAGCTGCTTCTTCTTCTGCTGCACCGCCTGCGTTGCCTGGTTCCAGTAATGTTGCTTTCACAACTATGCAGTCTGCTGTTGCTAAGAACATGGTGGAGAGTCTTTCTGTTCCTACGTTCCGTGTTGGATATCCGGTTACCACGGATGCTCTTGATGCTCTATATGAGAAGGTGAAACCTAAGGGTGTTACTATGACGGCTATATTGGCCAAGGCTGCTGCCATGGCACTTGTTCAGCATCCAGTTGTCAATGCCACCTGCAAAGACGGCAAGAACTTTCACTATAACAGTAACATTAATGTTGCTGTTGCCGTGGCAATCAATGGTGGTTTGATAACCCCAGTTCTTCAAGATGCTGATAAGTTGGACTTGTATCTGTTGTCCCAAAAATGGAAAGAGCTAGTTGGAAAGGCTCGTTCAAAGCAATTGCAACCTCATGAGTATAATTCAGGAACTTTCACGCTTTCAAACTTGGGTATGTTTGGAGTTGACAGGTTTGATGCCATACTTCCACCAGGCCAGGGGGCTATCATGGCTGTCGGAGCATCAAAGCCTACTGTTCTGGCTGATGCAGATGGATTCTTCAGGGTGAAAAATAAAATGCTGGTGAATGTAACAGCTGATCACCGAATAATTTATGGGGCTGACTTGGCTGCCTTCCTTCAGACATTCTCTAAAATCATTGAAAACCCAGAAAGCCTAACATTATAGTCATTCTTCAGAGATATATATTTAGTTCCACCGGAAGGTGTTTCTTGAGAAGCTGTTTGGCTCAATTCTTAAACTTTTTGCACTTTCTCTTTACACTTTCATTTTGTTATACTTTACTAGATCAATCACCCGGGAATTCTTAAACTTTGTACTTTTTCTTTGCACTTTCTTTTTGTTATATTTTACTAGCTCGATCACATAGAGATGCACATGCATAAGATTGTAATAATATTTGTGAGAGACCATAATTTAATTGTCTACTAATTTATTAATAAAGTT
TARGET=385,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6203
PRIMER_LEFT_PENALTY=0.327468
PRIMER_RIGHT_PENALTY=0.292834
PRIMER_LEFT_SEQUENCE=AAATCGTCTCGTGGATCAAATC
PRIMER_RIGHT_SEQUENCE=TCAGGTATTTCAGGAGGAGGAG
PRIMER_LEFT=332,22
PRIMER_RIGHT=602,22
PRIMER_LEFT_TM=60.327
PRIMER_RIGHT_TM=59.707
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.6203
PRIMER_LEFT_1_PENALTY=0.327468
PRIMER_RIGHT_1_PENALTY=0.292834
PRIMER_LEFT_1_SEQUENCE=AAATCGTCTCGTGGATCAAATC
PRIMER_RIGHT_1_SEQUENCE=CAGGTATTTCAGGAGGAGGAGA
PRIMER_LEFT_1=332,22
PRIMER_RIGHT_1=601,22
PRIMER_LEFT_1_TM=60.327
PRIMER_RIGHT_1_TM=59.707
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.6734
PRIMER_LEFT_2_PENALTY=0.074695
PRIMER_RIGHT_2_PENALTY=0.598673
PRIMER_LEFT_2_SEQUENCE=ATCCCTCCCTTTCTCAACTCTC
PRIMER_RIGHT_2_SEQUENCE=GCAGTTTCACCTTCAGAAACAA
PRIMER_LEFT_2=201,22
PRIMER_RIGHT_2=479,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=59.401
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.7082
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.598673
PRIMER_LEFT_3_SEQUENCE=CGTCACTCTTCCTTCAAAATCC
PRIMER_RIGHT_3_SEQUENCE=GCAGTTTCACCTTCAGAAACAA
PRIMER_LEFT_3=253,22
PRIMER_RIGHT_3=479,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.401
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.7082
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.598673
PRIMER_LEFT_4_SEQUENCE=CCGTCACTCTTCCTTCAAAATC
PRIMER_RIGHT_4_SEQUENCE=GCAGTTTCACCTTCAGAAACAA
PRIMER_LEFT_4=252,22
PRIMER_RIGHT_4=479,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.401
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_05215 Ps_contig_mira-and-tgicl-ass_franssen_36832
SEQUENCE=AATTAAGCTATTATCATAGTCAAAATATTTTAATGAGCACTATTTNAATTGATAATGTACTATACATTTCTTAACAATGAGTGGATCAAACTCAAATACTATAACTTAACATTGATAAGAACAAATGAACGATGAATTCTGGATTACAGTGCAGGTAGCAAGCATTGATAGTCAAATGAAATAATAAAACATCAACAATGTTGATGAACTGTTCAAATATACATAATATGTAGCAAAGCACAATCATAAACTAACTTATTATGCATTAGTTAAAGCATATATCCAGAAGTTATAACACAGCTAAAAACAGAGTGATAACAAATACCTCTACACATTATTGTTTCACTAAAATTCTTCATGTGAATCCGGAGAAGTCCTTTGGTGGTTCAATCCATATGCTTTCAAAAGGGTCTTGTTCGCCCCAGTCATGCCACTGCTTACGAGGATAGTAACGATAGTCGTCAAGAGAGAATGATACACAACGCTTTCCATAAAAACGAACTTTAGAAAAGTAGATACTGTTCCTCATTATTCCAGAGAGGTCAGTCCTTGAATGAGAAGACAAAAAACTAGCAAAAAGAGTTGCTCCATCTAGTGTTTTTAGCTCTTCCCATTCCATTAATGTCTGATCTAACTTGAAAATAATAGGGTTTTCACTAGAACATGTATAAATTACAATAACATCTCCTTCTTGCTCGGTCATAAATTTCCCCTTCCACCAATTTTTGGCAAAAAAATTCTCTGGGCATTTGGGTGGAGGTACTGATAGAACACTCCAAGTCCGCTCAGATGGATCAAATACCCCTAACCAACCGGTTAGACTCAGGCAATAAAAAAGTCCATTACAAAACACAAGCTTATTCCATATGCTACTGACAAAGGGCAAGCGGTTTTGGTAATTAACAGTTGTCCACTCAGTTGCCCCAGGGTGACATGTGCTGATGGCCACAACAGTAGGACTAACATGCTTAACAGTGAACAGCACGCAGTCAGGTGATGTTGGAGCACAGGAAAAAGCAACTATCTGGTATGTCATCTCAAATCTTGGCATTTTGATAATCTCCCGAGTAAAGGGATTAAAGAAAAACACTCGGTCAGTTCTTGGCCGGTATAGCAGTAACCAACCATCTTTTGTATAACAAACTCTAGATCCATTCAGCTCTGAAAACTCCAGGGAATACGTCTTCCGCTGCACAGGGTCATAGAATTCATACCACTGACCAAATTTTGGAAAATACATTAACCACGGTGATTGGTTAACCACCCGTACAGCATTGGCAACAGAATTCCAACTCTTGCAAACAACAGACGCACGAACATTATCGTCTAGTGCTAAACTACACATGATTATTTCTAAAAGCTCCGCAGGGAGATCAGACCATGTTTGCAACTCCAAATTATCATTTTTCACCTCTGTTTCTCCTCTTTTGCTGTTAGCGATGGTATCACTTAACAATTTCAACTTCCTCTTCTTTTTCCCTGACATTAATCGAAGTTTTCACTCTGACGAAGTCGCGGTTGTTGTTCTAGAGAGAGAGAGGAACCGCGAGAGAAAAGAGAGAGGAAGTGGAAAGTGTGTCAACCACCGARNANNGAAATCCCCGAC
TARGET=1529,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05216 Ps_contig_mira-and-tgicl-ass_franssen_36834
SEQUENCE=AATTCAGGGATGATTGAATTGTAATTATACAGTAAGAGTTCATCCGCGGTAACCACAGTGTTTGAACACATACAACAAAAATTCTACACTAAATTTATGTATTTAATAATGAGAAGTGAAGATGCTACTAAACTTTAACTAGATTCAAACTCAACCTTAAACTTTACCTATTCCATCATCACTTGAGCAAAATAAGCTACATGAGCCACAAAATCCAACACAAAACCCTTAAAGTCCAAAACTCCTTCAATAGCACACGGCCTTCAAATGGCATATGATATATAGACACCACACAAGTTCTAAGCAAATCGCAAACTAGAATGATAATCTAAGTTCCTGCCAAATGATTTTTCATCAAAAAAGATAAGTTACATTTTAAAGGAAACTCTCCAAAAACTGTTAAAATATAGGCATCAAATGAACCAAGGAAATCTAATATTCATCCTCACTATCTTCATCAATATTGGAATGCATCATCATGGATATCAAGGCTGCCTCCATAGGATCCAATTCATCAAAATCATCTGGGTCGAAATCATCTGGGCCAAAATCATCAGGGCCAAAATCATCCAAGTTATCTAGGGCCTCTGACATAGCAGCCATTCGAAAAACATTGAAGTTATCCCCCTCATCATCGGACTACAAAACGGACCCAAACGACGGGAACCGAACCACCAGCCAGAGAATCTGATAAAGCCATCATCTCTAATGGATCCATCTCATCAAAAAATTCTGAGTCCCTCATGATGGAATAAAATTCTCCGCTTGATAGATCGACTTCACTGAGCATGTCCAACATGTCATACATATCAAAATCGGTTTGTTTACGTTGAGGTGGAGGCCTGTGGTGTATCCATGTGTATGAGCCAGGCTTCACTGTATGCTTGTAAAAACAACTAGCCCCAAATGGACAATTCCCATTACCAGATTCGAAATGTTTGCAATCAATCAATCTGCAGTTTGCCTTGTAGTTATCAATAATTTCCTGTTTTTCTTCCTTTGTAGTATACCAAATACCACTTGGAATGACAAAATATGATAGTTGGCGGCAAACAGGGCAGGTTCTAACTGTATTGATATTATTACCCGTTTCAAATTCAGAGCTTGGGGCACTATTACGCCAATTGCGGATACATGACAAACAAAAAGCATGGTCACATTCTGGAAGCAACCCAAACTTCCGTTCTGATGGTTTGGGTTTGGAAAGGACATGCTCGAGACAAACATTGCATTCTATTTCTTCGCTATTTTTCAAAGCCAGAACGTACTTTTCCTTTTTGTCACAAGTCTTCAAATGATTTTCTTTTTCTCTTCTATCACTAGGATGCAAGCAATATTTTCTACAGTATAAACATTGATTTCCATGAACACGAGAACATCCATGTCCAAAGGGACAGTTTGCCGCGGCAAATTTACAAAACAAATGTCCATGCAGTCTAGCACTAGTACTACCAGTACTACTAGATTCACCAACATCGCTAGAGTCCTGATAGTTGTGTTGAAAACTCCTTACGCCATGCTTGCAAGGTGGAGGAGGAACTCTTGTAACCTTCGGAACCCAACTCGGTGCTAGTTTAACACCGTGAGTCACAGCACAATTTGGAATCAAAGACGCCGAAGATGATGCTTGAGAAGCTTTGACATGCTTATATCTGCACCGACTATCATAAGCACAAGAACCTTTCTGATAATAGGAGCAAATCTGTTTATCAACTGGATTATCCTTTCTTTGATGAGTATCCTTTCTCTGATGAGAAAAATCACATTGATCCCCTTTCAAACATATCCCTCGGGCATAGAACTTGCAAACCCTGGAGGACATGGTGGAAGAGGAGATTTTAGGGAATGTGAAAAACGGTGGAATAAAAAATAAAGAGGGAAGC
TARGET=942,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3247
PRIMER_LEFT_PENALTY=0.195574
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=ATCGGTTTGTTTACGTTGAGGT
PRIMER_RIGHT_SEQUENCE=AGCTCTGAATTTGAAACGGGTA
PRIMER_LEFT=814,22
PRIMER_RIGHT=1104,22
PRIMER_LEFT_TM=59.804
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.3980
PRIMER_LEFT_1_PENALTY=0.268895
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=AATCGGTTTGTTTACGTTGAGG
PRIMER_RIGHT_1_SEQUENCE=AGCTCTGAATTTGAAACGGGTA
PRIMER_LEFT_1=813,22
PRIMER_RIGHT_1=1104,22
PRIMER_LEFT_1_TM=60.269
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.4395
PRIMER_LEFT_2_PENALTY=0.054900
PRIMER_RIGHT_2_PENALTY=0.384647
PRIMER_LEFT_2_SEQUENCE=AATGGACAATTCCCATTACCAG
PRIMER_RIGHT_2_SEQUENCE=AAACCATCAGAACGGAAGTTTG
PRIMER_LEFT_2=905,22
PRIMER_RIGHT_2=1194,22
PRIMER_LEFT_2_TM=59.945
PRIMER_RIGHT_2_TM=60.385
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.4597
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.384647
PRIMER_LEFT_3_SEQUENCE=ACAATTCCCATTACCAGATTCG
PRIMER_RIGHT_3_SEQUENCE=AAACCATCAGAACGGAAGTTTG
PRIMER_LEFT_3=910,22
PRIMER_RIGHT_3=1194,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=60.385
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.5332
PRIMER_LEFT_4_PENALTY=0.054900
PRIMER_RIGHT_4_PENALTY=0.478252
PRIMER_LEFT_4_SEQUENCE=AATGGACAATTCCCATTACCAG
PRIMER_RIGHT_4_SEQUENCE=GTGACCATGCTTTTTGTTTGTC
PRIMER_LEFT_4=905,22
PRIMER_RIGHT_4=1157,22
PRIMER_LEFT_4_TM=59.945
PRIMER_RIGHT_4_TM=59.522
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_05217 Ps_contig_mira-and-tgicl-ass_franssen_36844
SEQUENCE=CGAAAACAAACAATACTAAGTAAATACTAAATATAACAAAACAAATATTAATATAATCNGAAAATTAAAGACTGCTTTAATTTCTTAGACCAAATTAATTACAACAAAATACTGTACAAAAGAAAAAACTACTAACTCCTTTATTCATCATCATCATCCTTCATCCCTCATTGGTGATGTGAACACGTGCACTTAGCTCGATTTTGTGAAAGAGTCTCAACTTGTTTTTGTAGGTCTTTAATGTACTCAACAGCTAAGTCCAACATATCTGATGTGTTTGTTTGCTTATCCATGTTTGGCACCAGATCTTGTAGTTTCCTCATTCGCTCGCTAATTTTAGTTCTTCTGACCCTTTCTGCAATGCTTCTCGGGTGAGTGGCGCAGCCGCGCTTGGCGCGGATTTTGCATGGAACAGAATCTGACAACTGTAAAAACTTCTCAATGGCTGCCAATTCCGCCGTGGTATTAGGCATACTCGTCTGATGAGCCAAAGGAGTGGCGGGAGTCTGGCCTCCTGTTTCATTCTTATTTTCAGCCGCATTTAAACCGGTAGAAAACGGTTTTACATCGTCATCATCTCTGTATCTTTTTAGACCGGTAATATTGTCCGATATCATTGCAGTATCCTCCCATGTAGAAACTAAAGGGAAACCCGGGATAAAATCATTGCCCCGGGTTTCAGCAAAACCTTCCGATTCTAGATTATTTTGTGGATTGCTACAATTCCCGATTTCACCTCCTAACACTGCTGAGTAGTTCGGAGCACTCGCATTCTTTAACCGCGCTGCAGAAGAAGAGAAATTTGCTTCTATGCTAGTGCTGTTAGCAGCTCCAATCGTTCCAATACCTCTCATAACAGCAAAACCAGTTTCAATGTTAATGTTTGAAAACAATCCAGCAGGTGAACTACCATGTCTAATAAGATTGGAACAGCTTCCTCTACCAGTCTTCATTTGATTTGGCAAAGGTGGTCTTCCAGAACTTTGGTAAGATTTCTGAGGACCAGAGGATCCATAATTATTCATATTACTTGGTTGTCGTTGAAGCTGTTGAAGATCCAAAGTTTCATTATTCATTGATGTCAAAGTTTGAGATTGTTGCTCAATCTCTTCCTTAACTATAGGAAGCTGTTGTTGAACAGTTAATAGATCCTGAGTCAGCGAATCATCATCTCCAGCAGCTACAGCAACCGCAACAGAAGCACTTTCAGCATCACCACCGCCACCACCACTACCACTACCACTAAGACTATTCATAAACCGAGCAAAAACTCGTTCCGTTTCAGGACTTGAAGGTCGGTTAAAAAGATGCTCATAGAACTCTTTATCAATAATATTAGAAAAATATGAACTTGGTGCAGATTTAAACCGAGTTAACCCGGAATTCATTTGAGGCTGGTGATGATGATTTTGATGATCAAGAAACATAGTTGGATGCTGCTGAAGATCTGACTCCATTTTTTCTTCCTCTACAAAAAATCCTCTTTGTCTGTGAGCACCGAACTGAAAAAATTTCTATGTTGCGTGTAGTGGTGAGAGTGGTGATGAAGAAAATAAGAAACCTGTATAGGAAGGTTCAAGAGAGAAGATAATAGTATAAAATAGCATTGGATTTTGTTGCTGTGGAGGTGCAGCCAATGAGGAGTCAA
TARGET=145,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05218 Ps_contig_mira-and-tgicl-ass_franssen_36845
SEQUENCE=TTTCATTTATCTTCTTATGAAACTAACCATGACAAAAAGCTCAACTACCTTAAAGCTATAAAACAAAAGAAATTGAAGAAGAATACATGGATTATAACTAACTTAAGACACAACCAATACATAACAAATGCATGATGTGACTGATCAAGTACTTTCTAATTGACCATTCTCCATATAACAATCAGAACATGCATGCACTTCAAACTTCATCATGCACTAATCAATTCATCAAAGCCTCATCAAGATTACAACATAACTCTCCATCAGTGGTGGTGGTGGTAATGAGGAGAAGGCGCGGTGGCGTTCACCTGATGCGGCGGCGGCATGGTTGGAAGAGACGACGGTGGAGGAAGACATTGGTAACACTTAGTGAAGAGCCCAAATGTATCAATCTGGTGTATGAAATTCACCATACTGGCCCACCCACCAAATCCAAATCCCACAATTAGGACCCACACCACTATAAATACATTGATCACAAATGTTCCTACCCATCTTCCAACAAATTTGGGAGGTTGTTCCACAGCATTCTGTCGAGCGGAGGAGGATTTAAAGGTGAAGATGTAAGCAAGGGCGGGGATGATATAAACAGTGAAACTGACGAGGAGAGATCCGACGGTGGAATTGATGGGTCCAAAGAAAGGGAATATAATAGCCAAGAACCAAATTGGAATGACCACTGGTAATCTCACCAATGCACGTTTGCACAAACTTTTACACTCATGCATTCCTATTGCTTTCTCCCAAACAAAGTATAATGGAGTGCATGCAAATCCAAATGTTATAAATTGGTGAATGAGCATTAAAATGACAGCCATGTCACGGAAGGGTGATTTTGGGAGTAGAGCAAAAGCATTAGAGTGATTTAGAAGCATGTCACCAAATGCCCAATAAACTGCTGCTGCTGATGGAAGGGTCAGTGTCAGAACATAAAGGGTAGCCATTAAATATATGGCTTTGAACTTTTGTGGCTTCCACATAGCGTGCATGATTTCCACAGTAACGGCATGGCCCCCAAATGTGTAGAGAATGTTTGTGGCCCAGTAAAATATAGCACCATTTTATTTGGACCCGAGTGCTTAACTCCTTCCATCTGACCGTGAAGG
TARGET=267,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4483
PRIMER_LEFT_PENALTY=0.183296
PRIMER_RIGHT_PENALTY=0.265053
PRIMER_LEFT_SEQUENCE=AGAACATGCATGCACTTCAAAC
PRIMER_RIGHT_SEQUENCE=GTTACCAATGTCTTCCTCCACC
PRIMER_LEFT=183,22
PRIMER_RIGHT=363,22
PRIMER_LEFT_TM=60.183
PRIMER_RIGHT_TM=59.735
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.4483
PRIMER_LEFT_1_PENALTY=0.183296
PRIMER_RIGHT_1_PENALTY=0.265053
PRIMER_LEFT_1_SEQUENCE=ACATGCATGCACTTCAAACTTC
PRIMER_RIGHT_1_SEQUENCE=GTTACCAATGTCTTCCTCCACC
PRIMER_LEFT_1=186,22
PRIMER_RIGHT_1=363,22
PRIMER_LEFT_1_TM=60.183
PRIMER_RIGHT_1_TM=59.735
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.4483
PRIMER_LEFT_2_PENALTY=0.183296
PRIMER_RIGHT_2_PENALTY=0.265053
PRIMER_LEFT_2_SEQUENCE=GAACATGCATGCACTTCAAACT
PRIMER_RIGHT_2_SEQUENCE=GTTACCAATGTCTTCCTCCACC
PRIMER_LEFT_2=184,22
PRIMER_RIGHT_2=363,22
PRIMER_LEFT_2_TM=60.183
PRIMER_RIGHT_2_TM=59.735
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.4524
PRIMER_LEFT_3_PENALTY=0.183296
PRIMER_RIGHT_3_PENALTY=0.269153
PRIMER_LEFT_3_SEQUENCE=AGAACATGCATGCACTTCAAAC
PRIMER_RIGHT_3_SEQUENCE=ACCAGATTGATACATTTGGGCT
PRIMER_LEFT_3=183,22
PRIMER_RIGHT_3=396,22
PRIMER_LEFT_3_TM=60.183
PRIMER_RIGHT_3_TM=59.731
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.4524
PRIMER_LEFT_4_PENALTY=0.183296
PRIMER_RIGHT_4_PENALTY=0.269153
PRIMER_LEFT_4_SEQUENCE=ACATGCATGCACTTCAAACTTC
PRIMER_RIGHT_4_SEQUENCE=ACCAGATTGATACATTTGGGCT
PRIMER_LEFT_4=186,22
PRIMER_RIGHT_4=396,22
PRIMER_LEFT_4_TM=60.183
PRIMER_RIGHT_4_TM=59.731
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_05219 Ps_contig_mira-and-tgicl-ass_franssen_36845
SEQUENCE=TTTCATTTATCTTCTTATGAAACTAACCATGACAAAAAGCTCAACTACCTTAAAGCTATAAAACAAAAGAAATTGAAGAAGAATACATGGATTATAACTAACTTAAGACACAACCAATACATAACAAATGCATGATGTGACTGATCAAGTACTTTCTAATTGACCATTCTCCATATAACAATCAGAACATGCATGCACTTCAAACTTCATCATGCACTAATCAATTCATCAAAGCCTCATCAAGATTACAACATAACTCTCCATCAGTGGTGGTGGTGGTAATGAGGAGAAGGCGCGGTGGCGTTCACCTGATGCGGCGGCGGCATGGTTGGAAGAGACGACGGTGGAGGAAGACATTGGTAACACTTAGTGAAGAGCCCAAATGTATCAATCTGGTGTATGAAATTCACCATACTGGCCCACCCACCAAATCCAAATCCCACAATTAGGACCCACACCACTATAAATACATTGATCACAAATGTTCCTACCCATCTTCCAACAAATTTGGGAGGTTGTTCCACAGCATTCTGTCGAGCGGAGGAGGATTTAAAGGTGAAGATGTAAGCAAGGGCGGGGATGATATAAACAGTGAAACTGACGAGGAGAGATCCGACGGTGGAATTGATGGGTCCAAAGAAAGGGAATATAATAGCCAAGAACCAAATTGGAATGACCACTGGTAATCTCACCAATGCACGTTTGCACAAACTTTTACACTCATGCATTCCTATTGCTTTCTCCCAAACAAAGTATAATGGAGTGCATGCAAATCCAAATGTTATAAATTGGTGAATGAGCATTAAAATGACAGCCATGTCACGGAAGGGTGATTTTGGGAGTAGAGCAAAAGCATTAGAGTGATTTAGAAGCATGTCACCAAATGCCCAATAAACTGCTGCTGCTGATGGAAGGGTCAGTGTCAGAACATAAAGGGTAGCCATTAAATATATGGCTTTGAACTTTTGTGGCTTCCACATAGCGTGCATGATTTCCACAGTAACGGCATGGCCCCCAAATGTGTAGAGAATGTTTGTGGCCCAGTAAAATATAGCACCATTTTATTTGGACCCGAGTGCTTAACTCCTTCCATCTGACCGTGAAGG
TARGET=896,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2938
PRIMER_LEFT_PENALTY=0.256503
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=TTTGGGAGTAGAGCAAAAGCAT
PRIMER_RIGHT_SEQUENCE=GGCCACAAACATTCTCTACACA
PRIMER_LEFT=834,22
PRIMER_RIGHT=1040,22
PRIMER_LEFT_TM=60.257
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.3321
PRIMER_LEFT_1_PENALTY=0.256503
PRIMER_RIGHT_1_PENALTY=0.075553
PRIMER_LEFT_1_SEQUENCE=TTTGGGAGTAGAGCAAAAGCAT
PRIMER_RIGHT_1_SEQUENCE=GGGTCCAAATAAAATGGTGCTA
PRIMER_LEFT_1=834,22
PRIMER_RIGHT_1=1072,22
PRIMER_LEFT_1_TM=60.257
PRIMER_RIGHT_1_TM=60.076
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.3660
PRIMER_LEFT_2_PENALTY=0.256503
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=TTTGGGAGTAGAGCAAAAGCAT
PRIMER_RIGHT_2_SEQUENCE=ACATTCTCTACACATTTGGGGG
PRIMER_LEFT_2=834,22
PRIMER_RIGHT_2=1032,22
PRIMER_LEFT_2_TM=60.257
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=12.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=0.3798
PRIMER_LEFT_3_PENALTY=0.256503
PRIMER_RIGHT_3_PENALTY=0.123278
PRIMER_LEFT_3_SEQUENCE=TTTGGGAGTAGAGCAAAAGCAT
PRIMER_RIGHT_3_SEQUENCE=CGGTCAGATGGAAGGAGTTAAG
PRIMER_LEFT_3=834,22
PRIMER_RIGHT_3=1099,22
PRIMER_LEFT_3_TM=60.257
PRIMER_RIGHT_3_TM=60.123
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=0.5096
PRIMER_LEFT_4_PENALTY=0.095574
PRIMER_RIGHT_4_PENALTY=0.414033
PRIMER_LEFT_4_SEQUENCE=AGAACCAAATTGGAATGACCAC
PRIMER_RIGHT_4_SEQUENCE=ATGTTCTGACACTGACCCTTCC
PRIMER_LEFT_4=658,22
PRIMER_RIGHT_4=930,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=60.414
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_05220 Ps_contig_mira-and-tgicl-ass_franssen_36847
SEQUENCE=GTCGGGGGGTACTGTATGAATTATCTATAGTTGAAGGTTTGAGTTTGAGTTTAGGTTGTGGTGTAGGTTGAGAAATCGAAAACAATGGGCTCAATTTCAACCTTGGCGTTTCCGCTTCTAGTCGGAGCCGGTGTCGGAGTCCCCTTTTCTCGTTCATCTTCTCTTCGAACACAACATTCTACGTCAATGTCGGTGGCAGCTTCTTCTTCTTCTTCTCAGGATGTTGCTGCAACTCAATTACTCACCTTTCTCGGTAAAGGTGGCTCTGGCAAAACCACCGCAGCTATCTTCGCCGCTCAGCACTATGCAATGGCTGGATTCAAGACATGTTTAGTGATACATAGCCAAGACGTCACTGCAGACTATCTCCTCAACTGTAAAATTGGAACTTCCCATGTCCTTTGCACCAACAACCTTTCGGCTGTTAGGTTGGAAACAACCAAAATGCTTCTTGAACCTTTAAAACTTCTCAAGCAAGCAGACGCCCAGCTTAATATGACACAAGGAACACTTGGAGGGATTGTTGGAGAAGAGCTTGGCATTATGCCTGGAATGGACTCCATCTTTTTAGTACTTGCACTTGAGAGGCTTGTAGGATTTTTGGGGATGGCACCTTCCAATACTCAACACGATAAATTTGACATAATAATATATGATAGTGTCAGCAGCGAGGAAACCTTGCGAATCATGGGTGGATGCAGTAAAGCAAGATTGTACTTGAAATATATCCGCACATTGGCTGAGAAAACTGACCTTGGGAGATTGGCTGCTCCTTCACTCCTCAGACTTGTGGATGAGGCCATGAGAATAAGTAGCACCAGATCTTATTTAAACTGGAGGATGAGTTCAGAAACATGGGACAATTTGGATCAACTTCTCGAGAAAGGATCTTCCGCCTTCTCAAACTCCCAGAGATTTGGTTGCTTCCTTGTGATGGATCCTAACAATCCAACCTCGGTCAATTCTGCATTACGTTATTGGGGGTGTACAATTCAGGCTGGTGCTCAAGTTTCAGGTGCTTTTGGACTCTCCTCACACCAACCAAAACTAGAATCATACGAAAGAGCAAACAAAAATCTTTCTCCTTTGCCTTCTGCTTTTATATCAAGTCCGTTAATGATTAGCCCAGTAGACTGGAACAGAGTTTACTGGACACTGCGAATCAAAATGCTAGGCATCTTCTTACTTCACTATTGAGCCAATCCAGTAATATGACATCATCAGTTAAGTTTGATGTCAAAAGAAAATCGGTTACTCTCTTCATGCCAGGTTTTGACAAATCAGAGATTAAGCTATACCAATATAGGGGAGGATCTGAGCTTTTGGTAGAAGCCGGAGATCAAAGACGTGTCATCGCCTTACCCCCAGAAATTCAAGGAAAGGTTGGCGGAGCCAAATTCCAGGACAGAAGTCTTGTTATTACATTGCTATAACAGTAAAAATATTACAGCCGAGGCATATTTTCTAATCCACATTTCTGCATGAATTGAGGCAATCAGTAAACTATGCAAGGAAATGTGTCACTTTTCATTCTCTACTTTGTAGTTTTTAGATAATTATTAGTAACTGTTGTATGTTGTATGTGAACTAACTGCTTTGTGCAGTTATGTAACTGCTGTTGGTAGTCAGAGTAATAAATCGCTAG
TARGET=200,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4137
PRIMER_LEFT_PENALTY=0.079714
PRIMER_RIGHT_PENALTY=0.334016
PRIMER_LEFT_SEQUENCE=ACATTCTACGTCAATGTCGGTG
PRIMER_RIGHT_SEQUENCE=GCAGTGACGTCTTGGCTATGTA
PRIMER_LEFT=173,22
PRIMER_RIGHT=358,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.334
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=186
PRIMER_PAIR_PENALTY_1=0.6551
PRIMER_LEFT_1_PENALTY=0.079714
PRIMER_RIGHT_1_PENALTY=0.575411
PRIMER_LEFT_1_SEQUENCE=ACATTCTACGTCAATGTCGGTG
PRIMER_RIGHT_1_SEQUENCE=ATAGTCTGCAGTGACGTCTTGG
PRIMER_LEFT_1=173,22
PRIMER_RIGHT_1=365,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.425
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.8117
PRIMER_LEFT_2_PENALTY=0.477653
PRIMER_RIGHT_2_PENALTY=0.334016
PRIMER_LEFT_2_SEQUENCE=AGTCCCCTTTTCTCGTTCATCT
PRIMER_RIGHT_2_SEQUENCE=GCAGTGACGTCTTGGCTATGTA
PRIMER_LEFT_2=137,22
PRIMER_RIGHT_2=358,22
PRIMER_LEFT_2_TM=60.478
PRIMER_RIGHT_2_TM=60.334
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.9381
PRIMER_LEFT_3_PENALTY=0.079714
PRIMER_RIGHT_3_PENALTY=0.858382
PRIMER_LEFT_3_SEQUENCE=ACATTCTACGTCAATGTCGGTG
PRIMER_RIGHT_3_SEQUENCE=GGTTCAAGAAGCATTTTGGTTG
PRIMER_LEFT_3=173,22
PRIMER_RIGHT_3=457,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=60.858
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.9644
PRIMER_LEFT_4_PENALTY=0.079714
PRIMER_RIGHT_4_PENALTY=0.884724
PRIMER_LEFT_4_SEQUENCE=ACATTCTACGTCAATGTCGGTG
PRIMER_RIGHT_4_SEQUENCE=GAGGAGATAGTCTGCAGTGACG
PRIMER_LEFT_4=173,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=59.115
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_05221 Ps_contig_mira-and-tgicl-ass_franssen_36848
SEQUENCE=AAATTAAACTAGGTYGAAAACTTGGTCTTATATTTATACCAACTCATACATTAAACAATCTATAGTAGTAATAATAATAATCACAATATACACAATACTTAATCAACAATATAATATATCATAAATATGAAAAACTAAAAAAAGAAAATGAAAAATTAGGAACAACATTTGTAATGATCGTTGTTGTTTTGTTGCGTAATATTCTCTGCGGTTTGTGCGAGTGATTGAAGATTACACCGAACAATTGTGTCAACAAACACACGAGTGTCCTCTTTAGTATTTCCCGGAGGAATATCCACCACGTAGGACTCAACAACCACCGTGCCGGAACTATCACCCTCGCCGGAGGGATGGAGTGTAGTAACGGAACGGTAGTTAGCAAGCCGGTGATCTCCACCAACAACGCTGAAACTGATGACATGACGGTCCTCATCGAGGATCTCGAGGCGTTCGGTGCTGCGGGCAGCAGGGAGGCCAGATATAAGATTGACCTCCCTGAGTGTGCCCACATCACCTTCGCCGTTGATGAGACTGCAGCTTTTGATGAAGTTTTTGTAGGCTTGTGGCTTGTCGAAGCGGCGAACGACGGACCAGACGGAGGCGATGGAGGCTGTTGTTTCCTGAACGACCGCCGAGCATAGTTGGTTGGAGGAGATGGGATGTGTGTGGTAGTGTGATATAGTATTAGGGATGGTGGTGACAGGGTTGACGAAGAGCGGGGTGTTTTCACCGGGGAGGTGATCATGGTGGTTGGTGACGGTTGGAGGGGTGGTGTGGTTGATTCTATGGAGGAGGAGAGAAGATTTTGGTGGATTTGGAGGCATTTTTTGATACTATGGATGAAGATAATTAAAAAGAAAAGAAGTTGTAATAGAAGTCGATGGATCTTGTTAGTTTGAGTGGTGATTATGTTGATTATGAAGGGTTTTGAGCAAAGATGAAGATGAAAGAAAAGATGTATTTTTTTTTAAAGATTATAAGGGGCCCGRTATGTTATGGTGGTTAGGTTATATAGTNATACTGAGTGNATGNGTTNTTTTTCTGGTGT
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05222 Ps_contig_mira-and-tgicl-ass_franssen_36858
SEQUENCE=CAGTTGGTTACTNTAACGCAGAGTACGCGGGGATCCCTCTCTACCTTCAACATTTCCAGCATTTTTCTCAATTTCTTTGTGATCATCTTCTTTCCATGGCCACCTTCATTTTCAGGTCTAACAAAATGGTGCATGCCTTGACCTGGTTTTGTCTCATGCTAATGATTCATAAGGGTGCTGCTTATGAGTTCATAGTTGGTGGCCAAAAGGGTTGGAGTGTTCCAAGTGATTCCAGTTATAATCCTTTCAATCAATGGGCGGAAAAGAGCCGTTTTCAAGTAGGAGACACCCTAGTGTTCAACTACCAATCTGACAAAGATTCAGTGCTATATGTAAAGAGTGATGACTATGCAAGTTGCAACACTGCTTCACCTTATGCAAAATACTCTGATGGTCACACAGTCATCAAACTCAACCAATCAGGGCCTCATTTCTTCATCAGTGGAAACAAAGATAACTGTGCCAAAAATGAGAAAGTAACAGTGATTGTTCTATCTGACAGAAGCAACAGCAACAGCGGTGGTTCATCAAACACTAACCAAACAAGCAATGCTTCTCCACCATCACCACAATCATCATCACCATCACCTCCTTCACCATCTCCTTCTAAGCCAGATGATCAATCTCCTTCTCCAGATACAAACCAAACACCTTCTACTAGTGATCATGGTCATCCTCCTCGCAACGGTGCTGCTTCGGTCTTTGTCAACCTAGCTGGTTCTGTGGGAACACTCATGGCTTCAGTTCTGATTTTATCTAAGTATGTTTAAGTGGTTGAGTATTGATTGATTGATTATTTTATTTTTTTGTTTTCTGTTCTATTTTCTATGTTTTGAGTTCAGTGTGAACTATGTGTTTGTTCAAAGTTGGGATGATGTTTGATATTTGATATTTGTTTGTGAAGATTGAAAAATAAGTTGATAACAATTTGTATACACTTTTCTTTGCTTCTTGTTCAATAATGTAAAAATGTTGTAATGTTAAAAATGAAATGAATTAAGCCCATTTTTNAAATAAGGTT
TARGET=781,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9147
PRIMER_LEFT_PENALTY=0.292834
PRIMER_RIGHT_PENALTY=0.621889
PRIMER_LEFT_SEQUENCE=CCTCCTTCACCATCTCCTTCTA
PRIMER_RIGHT_SEQUENCE=CATCCCAACTTTGAACAAACAC
PRIMER_LEFT=587,22
PRIMER_RIGHT=873,22
PRIMER_LEFT_TM=59.707
PRIMER_RIGHT_TM=59.378
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.9425
PRIMER_LEFT_1_PENALTY=0.320610
PRIMER_RIGHT_1_PENALTY=0.621889
PRIMER_LEFT_1_SEQUENCE=GCCAGATGATCAATCTCCTTCT
PRIMER_RIGHT_1_SEQUENCE=CATCCCAACTTTGAACAAACAC
PRIMER_LEFT_1=610,22
PRIMER_RIGHT_1=873,22
PRIMER_LEFT_1_TM=59.679
PRIMER_RIGHT_1_TM=59.378
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=0.9425
PRIMER_LEFT_2_PENALTY=0.320610
PRIMER_RIGHT_2_PENALTY=0.621889
PRIMER_LEFT_2_SEQUENCE=AGCCAGATGATCAATCTCCTTC
PRIMER_RIGHT_2_SEQUENCE=CATCCCAACTTTGAACAAACAC
PRIMER_LEFT_2=609,22
PRIMER_RIGHT_2=873,22
PRIMER_LEFT_2_TM=59.679
PRIMER_RIGHT_2_TM=59.378
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.9664
PRIMER_LEFT_3_PENALTY=0.344549
PRIMER_RIGHT_3_PENALTY=0.621889
PRIMER_LEFT_3_SEQUENCE=AGTGATCATGGTCATCCTCCTC
PRIMER_RIGHT_3_SEQUENCE=CATCCCAACTTTGAACAAACAC
PRIMER_LEFT_3=659,22
PRIMER_RIGHT_3=873,22
PRIMER_LEFT_3_TM=60.345
PRIMER_RIGHT_3_TM=59.378
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=0.9694
PRIMER_LEFT_4_PENALTY=0.347541
PRIMER_RIGHT_4_PENALTY=0.621889
PRIMER_LEFT_4_SEQUENCE=TGTCAACCTAGCTGGTTCTGTG
PRIMER_RIGHT_4_SEQUENCE=CATCCCAACTTTGAACAAACAC
PRIMER_LEFT_4=703,22
PRIMER_RIGHT_4=873,22
PRIMER_LEFT_4_TM=60.348
PRIMER_RIGHT_4_TM=59.378
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_05223 Ps_contig_mira-and-tgicl-ass_franssen_36862
SEQUENCE=AGTAATGAGAGACACACGAATTTGTTGGGACATTGAAGCTAAAGAAAACCTCATCTCTATCTCTTCTCCTCTCCACTCGTCCCTGCAACACCACCACTTCAAACTTCACACCCAGTTTTTTTCTTTCTCTTTTCTTTTTAGCGATGGCTTATATAAAATCTTGTATTTCTTATTCTGCTTTACCCTTCTTCCAACGGAGGGGTCTACCACCTGCCTCTTCTTCACCATTTCATCACACAGTTCATTCTTCAGGAACATTCATTCCCCAATGTTTAACACCAAAAATATTAAGTGCCAAGTTTTCAAAGAATGTCAGATTCTCTATTCCTAGAAGTACTACTTCTTCTTCTTCAAACTCCACTGATGATGATGACACCATTAATCAGACTAAGACTAAGAAAACACCATTTGGATATTCAAGGAAAGATGTTATATTAATTGGACTTGGTGTTACTTTTCTTGGCATTGGCTTAAAATCCGGATTAGAGTATGTTGGAGTTGATCCACTACAAGCAGGAAATGTGGTTCAACTTGTGCTGGTTTTGGGTCTCACAATTGGATGGATCTCAACATATATCTTTAGAGTTTCAAACAAGGAAATGACTTATGCGCAGCAGCTTCGCGACTACGAAAGCAAAGTCATGGAGAAACGATTAGAGTCCCTGACAGAAGCAGAGCTAGAAGTATTGCTTCAAGAAGTCGAGGAAGAGAAGAGTCGTTAGCTATAGCTAGTGTGATGAAGAGGTGAATCGAAGTAGTTTGTTTGTGTATAAAGAATCAAGTTTTACAGCAGTAATTAAACAGAAATGATAATCTCTCTATATGGACTAGTTTAGAAAGGTTTCTGCTATTTTGTTACAGTTCACTCTCTCAGACAAAAAATATAGAGAATTCCAAAAGTATAGTGTTTGGTATCTTAGCAATATGTATTATTATTCGCAAAACACATACAAAAGGGACACATTTTATGTGTGAAGATGTAAATCATACATAACTAGGTGTTTGTTAATCATTGCACTATATGAAAATGAAGTTTGCCATAATTATTGTT
TARGET=340,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3390
PRIMER_LEFT_PENALTY=0.299089
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=TTCTTCAGGAACATTCATTCCC
PRIMER_RIGHT_SEQUENCE=AGTTGAACCACATTTCCTGCTT
PRIMER_LEFT=244,22
PRIMER_RIGHT=531,22
PRIMER_LEFT_TM=60.299
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.3390
PRIMER_LEFT_1_PENALTY=0.299089
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=TTCTTCAGGAACATTCATTCCC
PRIMER_RIGHT_1_SEQUENCE=AAGTTGAACCACATTTCCTGCT
PRIMER_LEFT_1=244,22
PRIMER_RIGHT_1=532,22
PRIMER_LEFT_1_TM=60.299
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=289
PRIMER_PAIR_PENALTY_2=0.3731
PRIMER_LEFT_2_PENALTY=0.299089
PRIMER_RIGHT_2_PENALTY=0.073989
PRIMER_LEFT_2_SEQUENCE=TTCTTCAGGAACATTCATTCCC
PRIMER_RIGHT_2_SEQUENCE=CAGCACAAGTTGAACCACATTT
PRIMER_LEFT_2=244,22
PRIMER_RIGHT_2=538,22
PRIMER_LEFT_2_TM=60.299
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.4096
PRIMER_LEFT_3_PENALTY=0.081468
PRIMER_RIGHT_3_PENALTY=0.328094
PRIMER_LEFT_3_SEQUENCE=GCCTCTTCTTCACCATTTCATC
PRIMER_RIGHT_3_SEQUENCE=AGCCAATGCCAAGAAAAGTAAC
PRIMER_LEFT_3=212,22
PRIMER_RIGHT_3=470,22
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=59.672
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.4672
PRIMER_LEFT_4_PENALTY=0.299089
PRIMER_RIGHT_4_PENALTY=0.168114
PRIMER_LEFT_4_SEQUENCE=TTCTTCAGGAACATTCATTCCC
PRIMER_RIGHT_4_SEQUENCE=CACATTTCCTGCTTGTAGTGGA
PRIMER_LEFT_4=244,22
PRIMER_RIGHT_4=523,22
PRIMER_LEFT_4_TM=60.299
PRIMER_RIGHT_4_TM=60.168
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_05224 Ps_contig_mira-and-tgicl-ass_franssen_36862
SEQUENCE=AGTAATGAGAGACACACGAATTTGTTGGGACATTGAAGCTAAAGAAAACCTCATCTCTATCTCTTCTCCTCTCCACTCGTCCCTGCAACACCACCACTTCAAACTTCACACCCAGTTTTTTTCTTTCTCTTTTCTTTTTAGCGATGGCTTATATAAAATCTTGTATTTCTTATTCTGCTTTACCCTTCTTCCAACGGAGGGGTCTACCACCTGCCTCTTCTTCACCATTTCATCACACAGTTCATTCTTCAGGAACATTCATTCCCCAATGTTTAACACCAAAAATATTAAGTGCCAAGTTTTCAAAGAATGTCAGATTCTCTATTCCTAGAAGTACTACTTCTTCTTCTTCAAACTCCACTGATGATGATGACACCATTAATCAGACTAAGACTAAGAAAACACCATTTGGATATTCAAGGAAAGATGTTATATTAATTGGACTTGGTGTTACTTTTCTTGGCATTGGCTTAAAATCCGGATTAGAGTATGTTGGAGTTGATCCACTACAAGCAGGAAATGTGGTTCAACTTGTGCTGGTTTTGGGTCTCACAATTGGATGGATCTCAACATATATCTTTAGAGTTTCAAACAAGGAAATGACTTATGCGCAGCAGCTTCGCGACTACGAAAGCAAAGTCATGGAGAAACGATTAGAGTCCCTGACAGAAGCAGAGCTAGAAGTATTGCTTCAAGAAGTCGAGGAAGAGAAGAGTCGTTAGCTATAGCTAGTGTGATGAAGAGGTGAATCGAAGTAGTTTGTTTGTGTATAAAGAATCAAGTTTTACAGCAGTAATTAAACAGAAATGATAATCTCTCTATATGGACTAGTTTAGAAAGGTTTCTGCTATTTTGTTACAGTTCACTCTCTCAGACAAAAAATATAGAGAATTCCAAAAGTATAGTGTTTGGTATCTTAGCAATATGTATTATTATTCGCAAAACACATACAAAAGGGACACATTTTATGTGTGAAGATGTAAATCATACATAACTAGGTGTTTGTTAATCATTGCACTATATGAAAATGAAGTTTGCCATAATTATTGTT
TARGET=362,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3390
PRIMER_LEFT_PENALTY=0.299089
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=TTCTTCAGGAACATTCATTCCC
PRIMER_RIGHT_SEQUENCE=AGTTGAACCACATTTCCTGCTT
PRIMER_LEFT=244,22
PRIMER_RIGHT=531,22
PRIMER_LEFT_TM=60.299
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=0.3390
PRIMER_LEFT_1_PENALTY=0.299089
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=TTCTTCAGGAACATTCATTCCC
PRIMER_RIGHT_1_SEQUENCE=AAGTTGAACCACATTTCCTGCT
PRIMER_LEFT_1=244,22
PRIMER_RIGHT_1=532,22
PRIMER_LEFT_1_TM=60.299
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=289
PRIMER_PAIR_PENALTY_2=0.3731
PRIMER_LEFT_2_PENALTY=0.299089
PRIMER_RIGHT_2_PENALTY=0.073989
PRIMER_LEFT_2_SEQUENCE=TTCTTCAGGAACATTCATTCCC
PRIMER_RIGHT_2_SEQUENCE=CAGCACAAGTTGAACCACATTT
PRIMER_LEFT_2=244,22
PRIMER_RIGHT_2=538,22
PRIMER_LEFT_2_TM=60.299
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.4096
PRIMER_LEFT_3_PENALTY=0.081468
PRIMER_RIGHT_3_PENALTY=0.328094
PRIMER_LEFT_3_SEQUENCE=GCCTCTTCTTCACCATTTCATC
PRIMER_RIGHT_3_SEQUENCE=AGCCAATGCCAAGAAAAGTAAC
PRIMER_LEFT_3=212,22
PRIMER_RIGHT_3=470,22
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=59.672
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.4672
PRIMER_LEFT_4_PENALTY=0.299089
PRIMER_RIGHT_4_PENALTY=0.168114
PRIMER_LEFT_4_SEQUENCE=TTCTTCAGGAACATTCATTCCC
PRIMER_RIGHT_4_SEQUENCE=CACATTTCCTGCTTGTAGTGGA
PRIMER_LEFT_4=244,22
PRIMER_RIGHT_4=523,22
PRIMER_LEFT_4_TM=60.299
PRIMER_RIGHT_4_TM=60.168
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_05225 Ps_contig_mira-and-tgicl-ass_franssen_36863
SEQUENCE=TTAAGACGACATATCCACTTATGTTTCTTACACATATATAATGTATATACAGANACATAATNAACANAANATCAAATAAATATTAATAAATATNTCAAGACACAAGCCACATGAAGAAAGTTGACCAAAGAGTCACCGCAATTGATGCCAATGCATAGGTCCACAGCATTATCATAGAACATTCACTTTCACCTGCTCCAAATAATTGAGCTATAGTACCTATAGCCATAGCTGGTGGAAGTGCATATTGAAGAAGAAGAATGAACTGATATAAAGGATCAACTTGCACCAAACCCAAATGTGTTGCACCTTTAACAATTACAACACCAAAAATTGGTAGCATAATATATCTCACTGCTATGATTCCTATAATTGTCCAAAATGGAGTACTTGCTCCCTTCAATCCTTTAAGAAGGTTAGCACCCATTATAAGTGTTGTTATTGGAATTGCAGCATCACCTAACATGGAAGCAGAGTCTTCAATTACATGAAGCGGAGCATTGCTTCCAATCATTAAACTATGTAACTTAGGAACTACACCAACGATAAATCCAACAATCGCTCCAAGAGTTGCCGGCGAAAATACGCCTCTGAAATTTGGGTTGTCTAAAATCATTCCACACTTATGCATAATTTTATCAAAAATACGCACCTTCACCTCCTGCTCAGATTCATTCTTAGAAAGCAATAGTCTGTATGCTGAATTTATATTTTCTGAGCATCCCTCTATAGGTGATTCTGATACATCCTCTGAAGTCGCCAATGTGACAGAGTCGCTACTTTCGTTACCTTCTTTCTGAACATTACTCGAAGAAATCCGCATTATGTTGTACACATAAGTCCATATAAAAATAAGCTCCAATTGCCATAGAAAGTGAAGCATAAGCCATTCCATATTGATAGCAGACATCAGGATCTCCAAAAGGACTACCTTTGTCTTGACATATTGCTGGAATAATGATTATCGGCAAGTTTCCTAAATTTCCTGCGGAACAAACACCTACAATGAGACCTTCAATGTTTTTCGGAGGCCTGGTGAGTTTAATAGTGATCCATCCTAAGGCCGAGCCTAACAAAAATGTAACCAATATGTTCACCGGCATGAACCACAACAAAACAACATTCTCATAAGTGATTGTTTCTGCCAAATTCCCACCCACCATAGCAGGATTAAACACATAATGTACAAGATGATTGGTTTGATTCCTTGCGTCTTTTCCCAGCAAATTTATGTTATCCAATGCAAGAAGTAAACCAATTGCAGTGAGTAGTAGAACTTTGAAAATAAAGACAGGGAAAGAAGCAACAGAAAATAGCTCC
TARGET=250,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1395
PRIMER_LEFT_PENALTY=0.007205
PRIMER_RIGHT_PENALTY=0.132262
PRIMER_LEFT_SEQUENCE=ACCTATAGCCATAGCTGGTGGA
PRIMER_RIGHT_SEQUENCE=TCTGCTTCCATGTTAGGTGATG
PRIMER_LEFT=217,22
PRIMER_RIGHT=474,22
PRIMER_LEFT_TM=60.007
PRIMER_RIGHT_TM=60.132
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.2040
PRIMER_LEFT_1_PENALTY=0.007205
PRIMER_RIGHT_1_PENALTY=0.196811
PRIMER_LEFT_1_SEQUENCE=ACCTATAGCCATAGCTGGTGGA
PRIMER_RIGHT_1_SEQUENCE=TCTTAAAGGATTGAAGGGAGCA
PRIMER_LEFT_1=217,22
PRIMER_RIGHT_1=412,22
PRIMER_LEFT_1_TM=60.007
PRIMER_RIGHT_1_TM=60.197
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=0.2085
PRIMER_LEFT_2_PENALTY=0.011696
PRIMER_RIGHT_2_PENALTY=0.196811
PRIMER_LEFT_2_SEQUENCE=GCATAGGTCCACAGCATTATCA
PRIMER_RIGHT_2_SEQUENCE=TCTTAAAGGATTGAAGGGAGCA
PRIMER_LEFT_2=152,22
PRIMER_RIGHT_2=412,22
PRIMER_LEFT_2_TM=59.988
PRIMER_RIGHT_2_TM=60.197
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.2085
PRIMER_LEFT_3_PENALTY=0.011696
PRIMER_RIGHT_3_PENALTY=0.196811
PRIMER_LEFT_3_SEQUENCE=TGCATAGGTCCACAGCATTATC
PRIMER_RIGHT_3_SEQUENCE=TCTTAAAGGATTGAAGGGAGCA
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=412,22
PRIMER_LEFT_3_TM=59.988
PRIMER_RIGHT_3_TM=60.197
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.2756
PRIMER_LEFT_4_PENALTY=0.007205
PRIMER_RIGHT_4_PENALTY=0.268401
PRIMER_LEFT_4_SEQUENCE=ACCTATAGCCATAGCTGGTGGA
PRIMER_RIGHT_4_SEQUENCE=TTAAAGGATTGAAGGGAGCAAG
PRIMER_LEFT_4=217,22
PRIMER_RIGHT_4=410,22
PRIMER_LEFT_4_TM=60.007
PRIMER_RIGHT_4_TM=59.732
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_05226 Ps_contig_mira-and-tgicl-ass_franssen_36869
SEQUENCE=CNCGGGGACCGTTTTTTTCCCGCAATTCAGTGAACCAAGAGCTTTCTCACAAGATGAAGGTGAAGAAGGGATGTCTAGCAGTTCAAGTCGGTTCAGAGGAACACCACTCTCAACAAGATGCTACTGTCACTGATTCTCAAAGGTTTCTCATCCCTATTTCGTATCTCTATCATCCTCTTTTCAACAATCTTCTCGACATGGCTTACGAGAGTTATGGCTACAACACGGATGGCCCCCTCAAACTGCCTTGCTCGGTTGATGAATTTCTTCACCTACGGTGGCAGATTGAAAAAGAATCAACACACCACCACCACCAACACAACCACCATCATCACCATCACTATCTCCCTTACGCCTTGTATTTCAACTCTTGTTAATAATAATAGTAATAATAATGATAATACTCACTTTCTGTTGTTATGCTACTCTGTCTTTTATTTATATTTTTAATTTCTCTTGTTCTTTAATCTTAAGACATAGATGAGATGACTCTGCTTTTTGTATTTAATAAAATTACTAGAGAAATACTACTATA
TARGET=303,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1537
PRIMER_LEFT_PENALTY=0.052187
PRIMER_RIGHT_PENALTY=0.101479
PRIMER_LEFT_SEQUENCE=TCTAGCAGTTCAAGTCGGTTCA
PRIMER_RIGHT_SEQUENCE=GTTGAAATACAAGGCGTAAGGG
PRIMER_LEFT=73,22
PRIMER_RIGHT=367,22
PRIMER_LEFT_TM=60.052
PRIMER_RIGHT_TM=59.899
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=0.1537
PRIMER_LEFT_1_PENALTY=0.052187
PRIMER_RIGHT_1_PENALTY=0.101479
PRIMER_LEFT_1_SEQUENCE=TAGCAGTTCAAGTCGGTTCAGA
PRIMER_RIGHT_1_SEQUENCE=GTTGAAATACAAGGCGTAAGGG
PRIMER_LEFT_1=75,22
PRIMER_RIGHT_1=367,22
PRIMER_LEFT_1_TM=60.052
PRIMER_RIGHT_1_TM=59.899
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.1710
PRIMER_LEFT_2_PENALTY=0.052187
PRIMER_RIGHT_2_PENALTY=0.118777
PRIMER_LEFT_2_SEQUENCE=TCTAGCAGTTCAAGTCGGTTCA
PRIMER_RIGHT_2_SEQUENCE=TAGTGATGGTGATGATGGTGGT
PRIMER_LEFT_2=73,22
PRIMER_RIGHT_2=342,22
PRIMER_LEFT_2_TM=60.052
PRIMER_RIGHT_2_TM=60.119
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=270
PRIMER_PAIR_PENALTY_3=0.1710
PRIMER_LEFT_3_PENALTY=0.052187
PRIMER_RIGHT_3_PENALTY=0.118777
PRIMER_LEFT_3_SEQUENCE=TAGCAGTTCAAGTCGGTTCAGA
PRIMER_RIGHT_3_SEQUENCE=TAGTGATGGTGATGATGGTGGT
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=342,22
PRIMER_LEFT_3_TM=60.052
PRIMER_RIGHT_3_TM=60.119
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.1970
PRIMER_LEFT_4_PENALTY=0.095538
PRIMER_RIGHT_4_PENALTY=0.101479
PRIMER_LEFT_4_SEQUENCE=TCGACATGGCTTACGAGAGTTA
PRIMER_RIGHT_4_SEQUENCE=GTTGAAATACAAGGCGTAAGGG
PRIMER_LEFT_4=192,22
PRIMER_RIGHT_4=367,22
PRIMER_LEFT_4_TM=59.904
PRIMER_RIGHT_4_TM=59.899
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=176
=
PRIMER_SEQUENCE_ID=SSR_05227 Ps_contig_mira-and-tgicl-ass_franssen_36870
SEQUENCE=TCAACGCNRAGTACGNGGGTTACAAAAACCAAACCCAACCAAAACAAAACAAATCACATAGCAAAAACACCAAAATTGTCATGGCAACAAAAACTAAGAGCCACAACAGCGTTAGAAGACCAAACTACAACCATTTCCTTCCTTCCATAGACACTGACTCTGTCAACAAGTCATCACTCTTCGAGTTCGACGAGTCAGAACTCTACAACTCAGCTCGCTCCAATTCTAACTCCAACTCACCTGAATTTCGTAAATCCGTTATTGCTTCTCGTTTTCACGCCACTTCGTCTTCCACCGCTGGACGCGTCGGAGATCCGATGTCGCTGCCGGTTAACGTTCCGGATTGGTCGAAGATCCTTGGAGAAGAGAACAGACAGAACCGGAGGAAGAACTACGACGACAGCGATGACGACGGTGACGATGAGAGGGTTCCGCCGCATGAGTTTCTTGCTAGAACGAGAATCGCTTCGTTTTCGGTTCATGAAGGAGTTGGTAGAACTCTCAAAGGTCGCGATCTTAGTAGGGTTCGAAACGCGATTTGGGCCAAAACCGGTTTTCAAGATTGAGTTTAAAGTTTAAACCCGGTCGGATCGAAAATTGGAACATTATATTGGACTCCAATGAAATCATCGAAACTTGTTTTTATTTTTTGTGGAGTTGGTTTATTTTTGTTTTTATTATTACTTTCATCTATGTGTATGGGATGGAAAATATCTGAGGATTTTGTTTATTAATATCATTATGATTATGATTCTTCATTGACGTTGTCAACTCGGTTTGTAGAAGTAGAAGTAGTTCAATGATTGGTAGGGTTTTGGTTTTACTTTTATACATTCTTGGATGARGATAAATAATGGTGATTATTGTGTTTAAAAAATT
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05228 Ps_contig_mira-and-tgicl-ass_franssen_36874
SEQUENCE=CAACGCAGAGTACGCGGRGACTCGGAGTACAAAGTACAGACACCCTAACACCACACCACGCATACTCAAACCAAAACGAGAGGACCAAACCAAACTTAAAAATACACTCCGCTCAGCGTTCTGTTTATTTGAATTCACTCTCCACACTCCACACACACAACATGGCCGTCGTTGGTGTTCTCGCTTTACAGGGTTCTTTCAACGAACACATAGCAGCTCTCAAAAGGTTAGGTGTTAAAGGTGTTGAAATTCGAAAACCTGAACAGCTTCTCACTGTTAATTCCTTAATCATCCCCGGTGGTGAAAGCACCACTATGGCTAAACTCGCCGAGGCTTTCAACCTGTTTCCAGCTTTGCGAGAGTTTGTGCAAATGGGAAAGCCAGTTTGGGGAACCTGTGCAGGACTAATTTTCCTGGCAAATAAAGCTACAGGACAGAAGACTGGTGGACAAGAACTTGTTGGTGGACTTGATTGTACTGTACACAGAAATTTCTTTGGCAGCCAGATTCAAAGCTTTGAGACAGAGCTTCTAGTACCAGAGCTTGTGTCCAAGGAAGGTGGTCCTGAAACATTCCGTGGAGTTTTTATTCGTGCCCCGGGAATTCTTAATGTAGGGCCAGGTGTTCAAGTCCTAGCTGATTATCCTGTACCTTCTGACAAAGTACTGAGTTCCGATTCCTCTGTTGAAGGCAAAAAGGAGAATGCTGAAGAAGGCAGCAAAGTCATAGTTGCTGTGAGACAAGGGAACATACTGGCGACTGCTTTTCATCCGGAATTGACTGCTGATACTCGATGGCATAGTTATTTCTTAAAGATGGAAAGTTTGGTTGGAAAAGAGGCCTCAAGTAGCATTGTTCCAGAACAAGTCACACAAGTCGGTACAAGTTTTAAGCAACCACCTCTGCACGATCTTCCTATCTTTCAGTAGGACCAGAAACTGTTAAAAAATTATGAATGTTTTATTTATTTGTCAAACAATTTTCTCTTGGAAACAATACATGATAACCATTAGATCTTAGAAAATAGTTTGAAAATATTCTTTTGAGAGATAAACAATGCATAGACTGTGTTTTCTGTAGTTATCTATCTACATAGTTGCATTTCTATATTGTTTTTTCCCCCTACTTGTATTCTCNATCGTAATAGCGATACCTGGGTCGTTTTGTTGGTTTATACAGTATACAA
TARGET=150,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05229 Ps_contig_mira-and-tgicl-ass_franssen_36878
SEQUENCE=TAAAAAAAACTTTAAAATCTATATATAAAGTATGTCTCTTCTCAGTCTTTTATTCAACAATGTTACATATTTATAGAACATGACATTATATAAACTCAACTTAATATCATTAATTAAAACAAAGAAATCATCGTGACAAAAGATATTGTCACAATGAGCAAAAGAGATGCAAAGTATTGTGAAGCTGAAGATGCATCACTAGAAGTAGTTGAAGAAGAAGAAGACGACGGTGACGACGAAGGTGATGATACTGAGGAAGAAGATCCATTTCCTTTCACTATTTTGATCTTCACCTTCATGCCTTGACCACAATGACCTAAAGTACCACATGTGAAGTACCTTGTACCTGGTTTGTCCAATTTCACAACATCTTTTCCACTGCTTAAGGAGTTTAGGGAGGTGCTAATATCACATTTTGTGTAAGCACTTTCGTTGCCTAGTTCAACCACACTATGCATTGAAGAGTATTTGAAAACAAGTTGATCTCCAACTTTGAATGTTTGGCTTGAACTCCATGAATCAAAATCTGAGGATGGATCCCAACCTTGGTTTCCACCAACAACATGTTGTGTTGCTAAAACCTCTTTTGTGATCAAAGTTGCTATTAGCACCAACAAAATAATGTTCTTGCACCYCATGTTTTACTTGTTTTCTTCTTCTTGCTATACCACTCTCTTAGAGATTAATTTTYGTTTC
TARGET=211,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05230 Ps_contig_mira-and-tgicl-ass_franssen_36880
SEQUENCE=TGCTTGGAGGCCTTCTATCAAATGGAGATAGTACAAAAACTTCACCTTGTCCTTTTTCCAATTAATAAATTATTTACTATAAAATTGCAAGTGAATAATAATAATAATAATATTTGATCTTGCTGTATTTCTAGAATAAATTTATGGGGGCAGAGGAATTCATTATTTTTCTTCTATTTTGTGATAAAAAATTAGTGAGCCGAGTAATTACTCTAGTATATATAAAGGTGAATTTCTTTCTTTCTAATAATAATAAGATGATTTTATCTTTTTTTGCTTTTTGGCAATTCCAAATACATGTCATGTGACTAAAGTTTAAAAAGGTT
TARGET=94,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5102
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.498050
PRIMER_LEFT_SEQUENCE=ACTTCACCTTGTCCTTTTTCCA
PRIMER_RIGHT_SEQUENCE=AAATAATGAATTCCTCTGCCCC
PRIMER_LEFT=38,22
PRIMER_RIGHT=167,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.498
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=12.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=130
PRIMER_PAIR_PENALTY_1=0.5867
PRIMER_LEFT_1_PENALTY=0.088662
PRIMER_RIGHT_1_PENALTY=0.498050
PRIMER_LEFT_1_SEQUENCE=CTTGGAGGCCTTCTATCAAATG
PRIMER_RIGHT_1_SEQUENCE=AAATAATGAATTCCTCTGCCCC
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=167,22
PRIMER_LEFT_1_TM=60.089
PRIMER_RIGHT_1_TM=60.498
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=12.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=166
PRIMER_PAIR_PENALTY_2=0.8104
PRIMER_LEFT_2_PENALTY=0.312329
PRIMER_RIGHT_2_PENALTY=0.498050
PRIMER_LEFT_2_SEQUENCE=GAGGCCTTCTATCAAATGGAGA
PRIMER_RIGHT_2_SEQUENCE=AAATAATGAATTCCTCTGCCCC
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=167,22
PRIMER_LEFT_2_TM=59.688
PRIMER_RIGHT_2_TM=60.498
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=12.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=0.9839
PRIMER_LEFT_3_PENALTY=0.485895
PRIMER_RIGHT_3_PENALTY=0.498050
PRIMER_LEFT_3_SEQUENCE=CTTCACCTTGTCCTTTTTCCAA
PRIMER_RIGHT_3_SEQUENCE=AAATAATGAATTCCTCTGCCCC
PRIMER_LEFT_3=39,22
PRIMER_RIGHT_3=167,22
PRIMER_LEFT_3_TM=60.486
PRIMER_RIGHT_3_TM=60.498
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=12.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=1.4091
PRIMER_LEFT_4_PENALTY=0.911054
PRIMER_RIGHT_4_PENALTY=0.498050
PRIMER_LEFT_4_SEQUENCE=GCTTGGAGGCCTTCTATCAAAT
PRIMER_RIGHT_4_SEQUENCE=AAATAATGAATTCCTCTGCCCC
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=167,22
PRIMER_LEFT_4_TM=60.911
PRIMER_RIGHT_4_TM=60.498
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=12.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_05231 Ps_contig_mira-and-tgicl-ass_franssen_36880
SEQUENCE=TGCTTGGAGGCCTTCTATCAAATGGAGATAGTACAAAAACTTCACCTTGTCCTTTTTCCAATTAATAAATTATTTACTATAAAATTGCAAGTGAATAATAATAATAATAATATTTGATCTTGCTGTATTTCTAGAATAAATTTATGGGGGCAGAGGAATTCATTATTTTTCTTCTATTTTGTGATAAAAAATTAGTGAGCCGAGTAATTACTCTAGTATATATAAAGGTGAATTTCTTTCTTTCTAATAATAATAAGATGATTTTATCTTTTTTTGCTTTTTGGCAATTCCAAATACATGTCATGTGACTAAAGTTTAAAAAGGTT
TARGET=233,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05232 Ps_contig_mira-and-tgicl-ass_franssen_36880
SEQUENCE=TGCTTGGAGGCCTTCTATCAAATGGAGATAGTACAAAAACTTCACCTTGTCCTTTTTCCAATTAATAAATTATTTACTATAAAATTGCAAGTGAATAATAATAATAATAATATTTGATCTTGCTGTATTTCTAGAATAAATTTATGGGGGCAGAGGAATTCATTATTTTTCTTCTATTTTGTGATAAAAAATTAGTGAGCCGAGTAATTACTCTAGTATATATAAAGGTGAATTTCTTTCTTTCTAATAATAATAAGATGATTTTATCTTTTTTTGCTTTTTGGCAATTCCAAATACATGTCATGTGACTAAAGTTTAAAAAGGTT
TARGET=245,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05233 Ps_contig_mira-and-tgicl-ass_franssen_36883
SEQUENCE=ACACNGCCACATGGTATTGACCCNATTGGTGTGTAAACGGTGGTAGTAGGGGTTTTGGTTTATAGCGGCGGAATGGGAAAGCGAGAGAAGCAGAAGCAGAAACACGACAGAACTCAACGAGGAAGAGATTACTATCTTCAAGAGAATGACCCTGCACAACCCTTCTCTTCTACTTCAACGCTTTCACCCGCTGACAACGAAGAAGAAGAAGAAGAAGCAATAGATGACGCTGATTCTGACAACAACAACAATGAACAACACCCATCACACGATATGCCTTCAAAATTTCTCCTTTACCAACAATCTGTCCAGTCTCCCAAAGGAGACATTAGTTATTTGCAAAAGTTCTTTCTCATGTATGTTGGTGGAAGGGTGCCTCTCCATCTTCAAGAAGATTTCTGCGGCACTGCGTTTCTTAGTACAGAATGGCTCCGAAGCGATCCAAGAAAGACGGCTATAGGATTAGATTTGGATCTCGAGGCACTTACTTGGTGCCTGGAAAACAATATACCTAAAATCGGGGCTGATGGGTTTTCTAGAATATCACTCTTTCATGGGAATGTTCTACAACCTCTTCAGTCAAAACTTGTGGAAATGGACCCTGAGGAGCTGGTAAGGAACATTTCACTGTCACAGAATACAGAGAATCTGCAGGTCGATGTGCTTGAGTCGGATGTCCCAACAAGCTCTTCTGCTCAAGATGATAAGTTAACTGCGAAAAACTTTCCAATGAACGGGAGAGATATTGTGTGTGCATTTAACTACAGCTGTTGTTGTCTCCATAAACGTGCTGATCTAGTTTTGTATTTCAAGCATGTTCGTGATGCCTTGTCAACAAAAGGTGGAATTTTCGTGATGGATTTATATGGAGGTACATCTTCAGAGAACAAGTTGAAGCTTCAAAGAAGATTTCCTAATTTTACGTATGTATGGGAGCAAGCTGAATTTGATATTATTCAAACGGAAAACAARGGATTAGTCCTCCATTTTCATCTAGAAAAAAGGNGCAAAAGAAAGCTTCGCATGTCGTTTTCATACAGCTGGCGGCTAG
TARGET=199,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05234 Ps_contig_mira-and-tgicl-ass_franssen_36883
SEQUENCE=ACACNGCCACATGGTATTGACCCNATTGGTGTGTAAACGGTGGTAGTAGGGGTTTTGGTTTATAGCGGCGGAATGGGAAAGCGAGAGAAGCAGAAGCAGAAACACGACAGAACTCAACGAGGAAGAGATTACTATCTTCAAGAGAATGACCCTGCACAACCCTTCTCTTCTACTTCAACGCTTTCACCCGCTGACAACGAAGAAGAAGAAGAAGAAGCAATAGATGACGCTGATTCTGACAACAACAACAATGAACAACACCCATCACACGATATGCCTTCAAAATTTCTCCTTTACCAACAATCTGTCCAGTCTCCCAAAGGAGACATTAGTTATTTGCAAAAGTTCTTTCTCATGTATGTTGGTGGAAGGGTGCCTCTCCATCTTCAAGAAGATTTCTGCGGCACTGCGTTTCTTAGTACAGAATGGCTCCGAAGCGATCCAAGAAAGACGGCTATAGGATTAGATTTGGATCTCGAGGCACTTACTTGGTGCCTGGAAAACAATATACCTAAAATCGGGGCTGATGGGTTTTCTAGAATATCACTCTTTCATGGGAATGTTCTACAACCTCTTCAGTCAAAACTTGTGGAAATGGACCCTGAGGAGCTGGTAAGGAACATTTCACTGTCACAGAATACAGAGAATCTGCAGGTCGATGTGCTTGAGTCGGATGTCCCAACAAGCTCTTCTGCTCAAGATGATAAGTTAACTGCGAAAAACTTTCCAATGAACGGGAGAGATATTGTGTGTGCATTTAACTACAGCTGTTGTTGTCTCCATAAACGTGCTGATCTAGTTTTGTATTTCAAGCATGTTCGTGATGCCTTGTCAACAAAAGGTGGAATTTTCGTGATGGATTTATATGGAGGTACATCTTCAGAGAACAAGTTGAAGCTTCAAAGAAGATTTCCTAATTTTACGTATGTATGGGAGCAAGCTGAATTTGATATTATTCAAACGGAAAACAARGGATTAGTCCTCCATTTTCATCTAGAAAAAAGGNGCAAAAGAAAGCTTCGCATGTCGTTTTCATACAGCTGGCGGCTAG
TARGET=239,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05235 Ps_contig_mira-and-tgicl-ass_franssen_36888
SEQUENCE=GGAAACGGAATTGAATAAGCATAATGGATTATATGAAATTTTGAAGACTCCTAAACAGTTACAAAAGCCTAATTAAACCTAACGCACTCAATACAAGGAAGAAAAAAATGTGTACACTAACTCGGGTCCGATTGAACCGGGTCTAAAATCTACACTACTTTCTCTGTCTAGTTCTGTTTTCTTCCTCTTTCTTTTTTAATAAAAAAGGAAATTAATTAAATTAAATATAAAAAATAATAAATCAAAAACTAGGAACAACCGGAGAAGTACAAACCGGTTTCGGTTTTGGCTTCAATTTCAATTTCAACTTCAACACCACCAACAACCTCCTTCTTCCGCCGCATTTCAAGTACCTTCCGGTGGTGGTTGGAGTGCAATTCACTTGAAAATGTAGGGCTACAAGCGGGTCGATATTCGGGTAATAACCGACCCGATTTATAACGGACCCCACATGCATTGCAAAGCGTTTTTGCTCCAAGTGGTCCAGTTCTCCACTGAGGTGTCTTCTGAACACCGCAATGACTGCACCGTCGCGGCGCCAGAGCCAACGCACCGTGACCCGAACCATTGAAACATGTCATTTTCTTCGGTTTCCTCGCCGGAACCGAGTCAAAACTCTGTGGTAAGTTGGTGTAAATCAACAAGGGGCTAGTAGGAGAGGAAGAGGTTGAAGATGAAGTTGAGCTTGTAGAAGAATCCGTTAAAGAAGTTGACCCGAATGGCCAAACCCGAACACCGGTTCTTGTTCTTTTGCTTCTAGCTTTGGTCTGAACCGGAGTTTTGAAAGTAAAAACCGGGTTTTTCTTCTCCAGCTTCGGCTCAGGAAGAACCATCGGTTTTTTCTCCGTTGTTGTTGTTAAAGTTACAACCGGCATAGCAGCAGAGAATTCCGAGAAGGAATCAGAATCTTCAACAAAATGAGAAAGCCATTCCAAGTCCGCAACATCATCACTCGGAACGTTAAGGTCGCTGGTGGGCAAAGAACCGTAATCTTGTTGGAGAGAGAAACTGTTGTCAAGGTTAGAAATTTCATTGCTCTGTTTGAGAGTGACACAAACATAGTCTTGTTGTTCTTCCTCGTTTTGTTGTTCTTCATCGTCAACGTGAGAGAAATCAAGGAGGTCGTCAACGAAAAAATCGTCCGAGGTTGTTCCATTTGGTCCATTAAGTCCTGTAACCTCGTCGACAATTGTTTGAGGTACCATGTCTTTCCTTAAACTTGTTTTCAAGGCTGTTCCAACGCACTCCATTTCTGTTTTCTCAACCTGTTGAGGAGGTAGATGAGAAAAGAGATGAACTTGGGGAATGGTGGAAGAAGAAGAAGAAGAAGGTAAAGGATGAAATTGAAAGAGAAGAGGATAGGGAGTTTGGTAAAGCATTGAATAAGGGGTGAAAACGTCAAGGGATTTATTATTACTAATNAAGTGTCTGTTTCTTTCTCTCTCTAATATACCCTTTTCTTTCTCCCCCGA
TARGET=847,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4407
PRIMER_LEFT_PENALTY=0.072552
PRIMER_RIGHT_PENALTY=0.368190
PRIMER_LEFT_SEQUENCE=CGGTTCTTGTTCTTTTGCTTCT
PRIMER_RIGHT_SEQUENCE=CTTAACGTTCCGAGTGATGATG
PRIMER_LEFT=736,22
PRIMER_RIGHT=964,22
PRIMER_LEFT_TM=59.927
PRIMER_RIGHT_TM=59.632
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.4451
PRIMER_LEFT_1_PENALTY=0.072552
PRIMER_RIGHT_1_PENALTY=0.372557
PRIMER_LEFT_1_SEQUENCE=CGGTTCTTGTTCTTTTGCTTCT
PRIMER_RIGHT_1_SEQUENCE=CCTTAACGTTCCGAGTGATGAT
PRIMER_LEFT_1=736,22
PRIMER_RIGHT_1=965,22
PRIMER_LEFT_1_TM=59.927
PRIMER_RIGHT_1_TM=60.373
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.4743
PRIMER_LEFT_2_PENALTY=0.072552
PRIMER_RIGHT_2_PENALTY=0.401769
PRIMER_LEFT_2_SEQUENCE=CGGTTCTTGTTCTTTTGCTTCT
PRIMER_RIGHT_2_SEQUENCE=GACTTGGAATGGCTTTCTCATT
PRIMER_LEFT_2=736,22
PRIMER_RIGHT_2=937,22
PRIMER_LEFT_2_TM=59.927
PRIMER_RIGHT_2_TM=59.598
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.4775
PRIMER_LEFT_3_PENALTY=0.143968
PRIMER_RIGHT_3_PENALTY=0.333520
PRIMER_LEFT_3_SEQUENCE=GTCTGAACCGGAGTTTTGAAAG
PRIMER_RIGHT_3_SEQUENCE=TGTGTCACTCTCAAACAGAGCA
PRIMER_LEFT_3=765,22
PRIMER_RIGHT_3=1054,22
PRIMER_LEFT_3_TM=60.144
PRIMER_RIGHT_3_TM=59.666
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.5122
PRIMER_LEFT_4_PENALTY=0.143968
PRIMER_RIGHT_4_PENALTY=0.368190
PRIMER_LEFT_4_SEQUENCE=GTCTGAACCGGAGTTTTGAAAG
PRIMER_RIGHT_4_SEQUENCE=CTTAACGTTCCGAGTGATGATG
PRIMER_LEFT_4=765,22
PRIMER_RIGHT_4=964,22
PRIMER_LEFT_4_TM=60.144
PRIMER_RIGHT_4_TM=59.632
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_05236 Ps_contig_mira-and-tgicl-ass_franssen_36888
SEQUENCE=GGAAACGGAATTGAATAAGCATAATGGATTATATGAAATTTTGAAGACTCCTAAACAGTTACAAAAGCCTAATTAAACCTAACGCACTCAATACAAGGAAGAAAAAAATGTGTACACTAACTCGGGTCCGATTGAACCGGGTCTAAAATCTACACTACTTTCTCTGTCTAGTTCTGTTTTCTTCCTCTTTCTTTTTTAATAAAAAAGGAAATTAATTAAATTAAATATAAAAAATAATAAATCAAAAACTAGGAACAACCGGAGAAGTACAAACCGGTTTCGGTTTTGGCTTCAATTTCAATTTCAACTTCAACACCACCAACAACCTCCTTCTTCCGCCGCATTTCAAGTACCTTCCGGTGGTGGTTGGAGTGCAATTCACTTGAAAATGTAGGGCTACAAGCGGGTCGATATTCGGGTAATAACCGACCCGATTTATAACGGACCCCACATGCATTGCAAAGCGTTTTTGCTCCAAGTGGTCCAGTTCTCCACTGAGGTGTCTTCTGAACACCGCAATGACTGCACCGTCGCGGCGCCAGAGCCAACGCACCGTGACCCGAACCATTGAAACATGTCATTTTCTTCGGTTTCCTCGCCGGAACCGAGTCAAAACTCTGTGGTAAGTTGGTGTAAATCAACAAGGGGCTAGTAGGAGAGGAAGAGGTTGAAGATGAAGTTGAGCTTGTAGAAGAATCCGTTAAAGAAGTTGACCCGAATGGCCAAACCCGAACACCGGTTCTTGTTCTTTTGCTTCTAGCTTTGGTCTGAACCGGAGTTTTGAAAGTAAAAACCGGGTTTTTCTTCTCCAGCTTCGGCTCAGGAAGAACCATCGGTTTTTTCTCCGTTGTTGTTGTTAAAGTTACAACCGGCATAGCAGCAGAGAATTCCGAGAAGGAATCAGAATCTTCAACAAAATGAGAAAGCCATTCCAAGTCCGCAACATCATCACTCGGAACGTTAAGGTCGCTGGTGGGCAAAGAACCGTAATCTTGTTGGAGAGAGAAACTGTTGTCAAGGTTAGAAATTTCATTGCTCTGTTTGAGAGTGACACAAACATAGTCTTGTTGTTCTTCCTCGTTTTGTTGTTCTTCATCGTCAACGTGAGAGAAATCAAGGAGGTCGTCAACGAAAAAATCGTCCGAGGTTGTTCCATTTGGTCCATTAAGTCCTGTAACCTCGTCGACAATTGTTTGAGGTACCATGTCTTTCCTTAAACTTGTTTTCAAGGCTGTTCCAACGCACTCCATTTCTGTTTTCTCAACCTGTTGAGGAGGTAGATGAGAAAAGAGATGAACTTGGGGAATGGTGGAAGAAGAAGAAGAAGAAGGTAAAGGATGAAATTGAAAGAGAAGAGGATAGGGAGTTTGGTAAAGCATTGAATAAGGGGTGAAAACGTCAAGGGATTTATTATTACTAATNAAGTGTCTGTTTCTTTCTCTCTCTAATATACCCTTTTCTTTCTCCCCCGA
TARGET=1312,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0553
PRIMER_LEFT_PENALTY=0.054514
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=TTCCATTTGGTCCATTAAGTCC
PRIMER_RIGHT_SEQUENCE=TCAATGCTTTACCAAACTCCCT
PRIMER_LEFT=1150,22
PRIMER_RIGHT=1381,22
PRIMER_LEFT_TM=60.055
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.0871
PRIMER_LEFT_1_PENALTY=0.086339
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=CGTCAACGTGAGAGAAATCAAG
PRIMER_RIGHT_1_SEQUENCE=TCAATGCTTTACCAAACTCCCT
PRIMER_LEFT_1=1096,22
PRIMER_RIGHT_1=1381,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.1480
PRIMER_LEFT_2_PENALTY=0.147295
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=TCAACGTGAGAGAAATCAAGGA
PRIMER_RIGHT_2_SEQUENCE=TCAATGCTTTACCAAACTCCCT
PRIMER_LEFT_2=1098,22
PRIMER_RIGHT_2=1381,22
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=284
PRIMER_PAIR_PENALTY_3=0.1725
PRIMER_LEFT_3_PENALTY=0.171757
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=ACGCACTCCATTTCTGTTTTCT
PRIMER_RIGHT_3_SEQUENCE=TCAATGCTTTACCAAACTCCCT
PRIMER_LEFT_3=1239,22
PRIMER_RIGHT_3=1381,22
PRIMER_LEFT_3_TM=60.172
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=0.2475
PRIMER_LEFT_4_PENALTY=0.246726
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=AGAGAAATCAAGGAGGTCGTCA
PRIMER_RIGHT_4_SEQUENCE=TCAATGCTTTACCAAACTCCCT
PRIMER_LEFT_4=1106,22
PRIMER_RIGHT_4=1381,22
PRIMER_LEFT_4_TM=60.247
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_05237 Ps_contig_mira-and-tgicl-ass_franssen_36890
SEQUENCE=ATGGGGATTTGGCGTAATAGAAGAGAAAAAGAAGAAGAAGAAGAGAGCTAAGAGTTTGGAAACTGGAAATTGAAAGCAATGGACGATTACACGAGAGAGATGATGGACCTCAAAACCCTCGTCACTCGCACTCTCGAGAAGAAAGGCGTTCTCGCTAGGATCCGAGCTGAATTAAGAGCTAGTGTCTTTGAAGCTATTGAAGAAGAAGATCGTGTCATTGAAAAGGATCAAGCTTTGCCTCCTGCATTGTTAGGTAGCTGTAACGATCGTGCTAAACAGCTTCACGCTTCTCCTTCTGGTAGACTTCTTACTGCACTGGTATGTGAATACCTTGACTGGGCACAGCTCACTCACTCGCTAAAAGTTTATCTTCCAGAATGTAATTTGGAGAAAGATTTTTGGAAGTCTGAGTTGAAAGAATTTAGTAGCAAAAATGGATATGATCTTAATAGAAATGGAGACAGCCCTGTGCTCTTGGATGTGCTTGAAGGATTCTTGAAATTTGAGAACTTATCCCAAGCAAGGGCTAGTGGTAGGAGGTTCACTACTTCAGAAACTGAGCCTTTGCCAAACTCAGAATCACGGAACACACGAAGGCATTCTTCATCATCTGTGGCTGGTGGTTTACCTCCACTAGGAAGGGCTGTTCCCTCATCTCAAGCATCTGATAGAAGAGGAGGATCCTCCGGGTCTGCATACAGGAAGGATGAGTACAATTGGCGATATGACAATGAAGAACTTCCCGAAGATGTAATTCAAGCATCAAATGCTTTGGAAAATCTTCAATTGGACAGAAAAGCTAGGAATCTAACATCATCTTGGCGACACGCTGCTGATGAAACCAGCGAGGACGATGGCAGGTCTGACCATGCTTAGGGACAAATATTGCCTATATTGTATAACCATATTCTGTCCCTGTATTTTATTGTGTATTCTGAATTTTCTTCCTTTGGTGATGATTTAATGAGCCCAATTTTATGTAAGACTATATCATGATATGGTTTAGCGTTTTAAGTGAGCTTTTTCCTCTCAAAAAATNACCCTTTTGGTTACT
TARGET=29,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9963
PRIMER_LEFT_PENALTY=0.855463
PRIMER_RIGHT_PENALTY=0.140792
PRIMER_LEFT_SEQUENCE=GGGATTTGGCGTAATAGAAGAG
PRIMER_RIGHT_SEQUENCE=AGGTCCATCATCTCTCTCGTGT
PRIMER_LEFT=3,22
PRIMER_RIGHT=109,22
PRIMER_LEFT_TM=59.145
PRIMER_RIGHT_TM=60.141
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=1.0510
PRIMER_LEFT_1_PENALTY=0.855463
PRIMER_RIGHT_1_PENALTY=0.195574
PRIMER_LEFT_1_SEQUENCE=GGGATTTGGCGTAATAGAAGAG
PRIMER_RIGHT_1_SEQUENCE=AGCTACCTAACAATGCAGGAGG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=258,22
PRIMER_LEFT_1_TM=59.145
PRIMER_RIGHT_1_TM=59.804
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=1.1782
PRIMER_LEFT_2_PENALTY=0.855463
PRIMER_RIGHT_2_PENALTY=0.322707
PRIMER_LEFT_2_SEQUENCE=GGGATTTGGCGTAATAGAAGAG
PRIMER_RIGHT_2_SEQUENCE=CTTTCTTCTCGAGAGTGCGAGT
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=144,22
PRIMER_LEFT_2_TM=59.145
PRIMER_RIGHT_2_TM=60.323
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=1.1813
PRIMER_LEFT_3_PENALTY=0.855463
PRIMER_RIGHT_3_PENALTY=0.325886
PRIMER_LEFT_3_SEQUENCE=GGGATTTGGCGTAATAGAAGAG
PRIMER_RIGHT_3_SEQUENCE=TGTTTAGCACGATCGTTACAGC
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=277,22
PRIMER_LEFT_3_TM=59.145
PRIMER_RIGHT_3_TM=60.326
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=1.3252
PRIMER_LEFT_4_PENALTY=1.184405
PRIMER_RIGHT_4_PENALTY=0.140792
PRIMER_LEFT_4_SEQUENCE=GGGGATTTGGCGTAATAGAAG
PRIMER_RIGHT_4_SEQUENCE=AGGTCCATCATCTCTCTCGTGT
PRIMER_LEFT_4=2,21
PRIMER_RIGHT_4=109,22
PRIMER_LEFT_4_TM=59.816
PRIMER_RIGHT_4_TM=60.141
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_05238 Ps_contig_mira-and-tgicl-ass_franssen_36893
SEQUENCE=AAGGCCTGATTTCCCTAGAAACCAGTTCTTTGTCTTTTTTGCACATTCAGCTCCTCTTTGAAGCCTGTCCGCCCAAAGCATCATTTTCTCCAATTTCTCTGGTGATATGGATGACATGGCTTGCTGAGCTTTTTCGGCATCTTCTGGTGAAAGATCGAAACCAAATTGTTTTCCCATGTTTGCCATCATTTCTGGGCTCATATTTTTAACCATTGATGTGAACATCTGCCGCATAGCTGGATCTTTCATCTGGTTTCTCATCTGTTCTTGTAAATCAGTACTTGAAGATGGGAAGTTTGGCTCTGAAGCATTTATAATATTTGAAAACGCATTGTCAGAAGAACTTGATTCTCCAAAAGCACGAGTTGTGCTTGAGGATGGGAAGTTTGACTGTGAAAAATTTCTTTCTTTCTTGTCTACAGCTGCTGCTGATGGAATAGATTGTTTCCCTTTCATTAAGGATGCCATTTCAAACATCTTCTTAAGGTCATCTGGTGGCATTTTACTTATCATATCACTTGCTGTTTTAAACATATCAGGTGTCACATTGGGAGGAATTGATCCAGAGTTTAAAGGAGAATCAGAGGAACCTCCTCTGAGAAATGGGTTGTTCCCTTGAAATGAAGACGCCATGTCGAGCATTTTTTGAAGTTCCTCAGGTGACATCTTGCCGATCATATCCGAAGACGCTTTAATCATATCAGGAGACACGTCTTGGGATTGTCCCGTGTTCAAAGAAGCCAGAGTGGTAGGATCAGCTTTGGAAATAAAATTTTGGAATGATCTGATGGCTTCTGGATCTTTCTTCAGTGTATCTATACTGTCTGAATTTGATTTTAGATTTCCATTGTTAGCTATATTGTAACTCTTAGAGTCGCCAGACTTTTTTTGGTTGATCAAATATTTGTTCCGAAGAAGAGCTTCTGTTGTTTCCAGAAGGCACATTCTCGATTTCTTCAGT
TARGET=401,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2101
PRIMER_LEFT_PENALTY=0.079714
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=ACGCATTGTCAGAAGAACTTGA
PRIMER_RIGHT_SEQUENCE=GCCACCAGATGACCTTAAGAAG
PRIMER_LEFT=326,22
PRIMER_RIGHT=498,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.2126
PRIMER_LEFT_1_PENALTY=0.079714
PRIMER_RIGHT_1_PENALTY=0.132857
PRIMER_LEFT_1_SEQUENCE=ACGCATTGTCAGAAGAACTTGA
PRIMER_RIGHT_1_SEQUENCE=CAATCTATTCCATCAGCAGCAG
PRIMER_LEFT_1=326,22
PRIMER_RIGHT_1=443,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=0.2143
PRIMER_LEFT_2_PENALTY=0.081468
PRIMER_RIGHT_2_PENALTY=0.132857
PRIMER_LEFT_2_SEQUENCE=GCTGGATCTTTCATCTGGTTTC
PRIMER_RIGHT_2_SEQUENCE=CAATCTATTCCATCAGCAGCAG
PRIMER_LEFT_2=235,22
PRIMER_RIGHT_2=443,22
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.2320
PRIMER_LEFT_3_PENALTY=0.101680
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=GAAGTTTGGCTCTGAAGCATTT
PRIMER_RIGHT_3_SEQUENCE=GCCACCAGATGACCTTAAGAAG
PRIMER_LEFT_3=291,22
PRIMER_RIGHT_3=498,22
PRIMER_LEFT_3_TM=59.898
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.2345
PRIMER_LEFT_4_PENALTY=0.101680
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_LEFT_4_SEQUENCE=GAAGTTTGGCTCTGAAGCATTT
PRIMER_RIGHT_4_SEQUENCE=CAATCTATTCCATCAGCAGCAG
PRIMER_LEFT_4=291,22
PRIMER_RIGHT_4=443,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_05239 Ps_contig_mira-and-tgicl-ass_franssen_36896
SEQUENCE=TATCGANGGAGAAATTGAAGAATGGAAACGGATAGCGTAAACTAGAGGTGGTGCGCGGTGAGGCCTGTATTAGCAATGACTTCCCCTTACACTTGCACTTGCAGTGGCAATTCCGTAAATTTCTTCCCAAACAAACGGAACGTTCATTACGGAGGTGGCGCGCTTTCGAATTCTAATCTCACCGCCACCACCAGGTTCAGACAACGGCCATTCACGGTGGTCGCGCTCTCTCTTTCTCAATCGAGAAGGTCCGCCGCGATTCTCATTTCATCCTTGCCTTTCACCTTCATTTTCCTCTCTCCGCCGCCTCCAGCGGAGGCCAGAGAGAGACGCAAAAAGAAGAACATTCCCATTGATGATTATCTCACTTCCCCTGATGGATTGAAATACTATGATTTCCTTGAGGGGAAAGGTCCAATAGCTGAAAAAGGTTTAACTGTTCAGGTGCATTTTGATTGTTTATATCGCGGAATTACAGCTATTTCAAGTCGAGAATCTAAACTCCTAGCTGGAAATCGTGTTATTGCTCAGCCATATGAATTCAAGGTGGGAGCTCAACCAGGAAAGGAAAGGAAGCGAGAATTTGTAGACAACCCAAATGGTCTATTCTCCGCACAAGCATCACCAAAACCTCCACCAGCCATGTACACAATAGTTGAAGGAATGAGAGTCGGAGGGAAGCGAACTGTGATTGTTCCTCCTGAAAAAGGGTATGCACAAAAGGGAATGAATGAGATTCCGCCTGGAGCTACATTTGAGCTAAATATTGAACTTTTGCAAGTGTCATCTTCCTGAAGATTGAGCTTGAGTTGATTCTTATGAAGAACTTGATCATTTCAATTCAGAATTTGTACCTTATGAGGCTACCGCCTACGGCGATTCTTTTCACATTGTCGACTAGGGAGATTTTTTCTCTTTCACAAAATGATTGTTTTGTATTATACTTGTCTTCTTTCTTGTAACCAAAAATACTCGTCTTTCTTCTCTCCTGGCTGCAATTTGAGAGTGATCTCAGTATATATTTGGATCACTTTTTGTATATTATGGAATATAGAAGCTTTAATTTTTAACTTTTGATTGTTATTTACCAGAATCTTTAAAGAATTTACGCTTGAGATCAATTTGAAGAACGAAGACTAAAATAGGAAAGATTACTCGAAGTACAATTACATTACGAA
TARGET=561,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1570
PRIMER_LEFT_PENALTY=0.130883
PRIMER_RIGHT_PENALTY=0.026163
PRIMER_LEFT_SEQUENCE=TGGAAATCGTGTTATTGCTCAG
PRIMER_RIGHT_SEQUENCE=TACCCTTTTTCAGGAGGAACAA
PRIMER_LEFT=509,22
PRIMER_RIGHT=712,22
PRIMER_LEFT_TM=60.131
PRIMER_RIGHT_TM=59.974
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=0.2428
PRIMER_LEFT_1_PENALTY=0.216588
PRIMER_RIGHT_1_PENALTY=0.026163
PRIMER_LEFT_1_SEQUENCE=AAACTCCTAGCTGGAAATCGTG
PRIMER_RIGHT_1_SEQUENCE=TACCCTTTTTCAGGAGGAACAA
PRIMER_LEFT_1=498,22
PRIMER_RIGHT_1=712,22
PRIMER_LEFT_1_TM=59.783
PRIMER_RIGHT_1_TM=59.974
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=215
PRIMER_PAIR_PENALTY_2=0.2778
PRIMER_LEFT_2_PENALTY=0.156135
PRIMER_RIGHT_2_PENALTY=0.121635
PRIMER_LEFT_2_SEQUENCE=AGCTGGAAATCGTGTTATTGCT
PRIMER_RIGHT_2_SEQUENCE=CCTTTTGTGCATACCCTTTTTC
PRIMER_LEFT_2=506,22
PRIMER_RIGHT_2=723,22
PRIMER_LEFT_2_TM=60.156
PRIMER_RIGHT_2_TM=59.878
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=0.3382
PRIMER_LEFT_3_PENALTY=0.216588
PRIMER_RIGHT_3_PENALTY=0.121635
PRIMER_LEFT_3_SEQUENCE=AAACTCCTAGCTGGAAATCGTG
PRIMER_RIGHT_3_SEQUENCE=CCTTTTGTGCATACCCTTTTTC
PRIMER_LEFT_3=498,22
PRIMER_RIGHT_3=723,22
PRIMER_LEFT_3_TM=59.783
PRIMER_RIGHT_3_TM=59.878
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.3461
PRIMER_LEFT_4_PENALTY=0.130883
PRIMER_RIGHT_4_PENALTY=0.215198
PRIMER_LEFT_4_SEQUENCE=TGGAAATCGTGTTATTGCTCAG
PRIMER_RIGHT_4_SEQUENCE=ACCCTTTTTCAGGAGGAACAAT
PRIMER_LEFT_4=509,22
PRIMER_RIGHT_4=711,22
PRIMER_LEFT_4_TM=60.131
PRIMER_RIGHT_4_TM=60.215
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_05240 Ps_contig_mira-and-tgicl-ass_franssen_36908
SEQUENCE=AAACTTAAATGATAATACTTCTTCCAAAGTGTTTCATTAAGATTTGACATCTATCATAGCGTTTTAACAATACGCTGCCGACAAATTAGCAAACAATAACTGCTACACTTTTCCTGTTTCCGATTTGTACTAATTTGTAAGCAAACATAGTTAAGAAAATGCAAATTCAATTGATACTTGAAGTCAAAAAGAATACAATTAGTGGAAAGAAAAGAACTGAATTAAACCAACAGTTAAATATACATAAGGTGAATATGGTGAGCCTTGCTTTCATCTGATTAGAATTTTGCTTAGCTTGTAATTGTTCAAACAATATCATTGATCAAACGCTTATGACGATGATAATGCTTTTCCATGAAAACTCTAGAAGCACCTTCAACAGTTCTACGCCATGTTTTTTGAATAGTTCCCAAGATTGATGACTTGTTTCTGTTATCTCTAGCCCTAAGCTGCCGGAGCAACACCGACCTCCGGTTTACAGTTGGCGGCAACAGCGACCTCCTTTCAACAACGGCGGCGCCGGCTTTCGAAGGGCTCTGGCTCTCGCGTTGATTCTCCTTCATCTTCTTCCTGCGTTTGATAATACGYAGCTCTTTCTTCTCGTTGGTACCCACTTCAGCAAATTTTCGAAGTAGTTCATCGGAAGATTTCTTAGTTACTCGTTGAACCTTAGAATCGGCGGTGGTGGTGGTTGTAAGGGTGGTAGCAGTTGGAGAGATAGATATAGATGTAACAGTAGGGATGGTTTCCATTGCAAGAGAGTAAGCAAAATATTAATTAATGAAAATGAAAAAGTAAG
TARGET=681,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05241 Ps_contig_mira-and-tgicl-ass_franssen_36915
SEQUENCE=ATGAGAGTGTGACCAAGTGTTGCTTAGTATCCAATCNAAGACTAAATCATAACATAACAAAGAAAATCCAAATTAAGCTTCTTTTAAGATCTTTCAAATTGATGCATCAGAAAAAGTAAAGAAGAAGAAGAAGAAGAAAATGGCGGAAGAAAAAGAATCAACGTCAATTCCTCTGAGTCAAGCTGATAATGTCTCTTGCGAAGATCCCGAAGATCCAGCTAAGTCTCCACCAACCTCTCCCAACTCTTCTACTCGCAGGGCTTGCTGCTTTGTTCTTCAGAGTTGGGTCTCAAAGAAGTTTATGACTGGAATTGTTGTTCTATTCCCAGTTGCAGTTACCTTTTTTATTACATGGTGGTTTATTCAGTTTGTCGATGGTTTCTTTAGTCCGCTATACTCCAGTTTTGGCATTGAAATATTTGGACTTGGTTTTATTACATCGCTAGCTTTTGTATTTGTTATTGGTGTTTTTGTTTCGTCGTGGATGGGGGCCACTGTCTTCTGGATTGGAGAATGGTTAATAAAGCAGATGCCCCTTGTCAGACATATATATTCTGCATCCAAGCAGATTAGTGCTGCAATTTCTCCAGATCAAAATACCACTGCCTTTAAAGAGGTAGCAATTATCCGTCATCCGCGTGTTGGTGAATATGCTTTTGGCTTCATTACATCAACTGTTACTCTACAGCAAGAAAATGAAGATGAAGAGCTTTGTAGTGTTTTTGTCCCTACGAACCATCTGTATATTGGCGATATAATTTTGGTTAACTCCAAAGATGTAATAAGACCTAATCTTTCCATTCGAGAAGGCATAGAAATCATTGTTTCTGGAGGAATGACAATGCCACAGTTGATATCTCCCATCGAAAGAGTTACTCGACCCAATGAGAGAATATCTTTGAACAGAATTGGATAAAGGAATGCACCCGCTGTTTAAGATTGATTTGTAGTTATCGTCCATTATTATACCATGCATGGGTCATATGGTGATGAGAATTGTATTCTGAATCTCGTTGTGATGGAGTCTGTATACGAAAGTTGTTGAATATTTATAGTTTCTATCGAAGTTCAAAAAAGAGATACAATGTAGTTATTACAATATTTTTATCATTTTCTTTATAGTTGACNANCTTTACGTTT
TARGET=119,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6842
PRIMER_LEFT_PENALTY=0.575240
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=GAGAGTGTGACCAAGTGTTGCT
PRIMER_RIGHT_SEQUENCE=GACGTTGATTCTTTTTCTTCCG
PRIMER_LEFT=2,22
PRIMER_RIGHT=164,22
PRIMER_LEFT_TM=59.425
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.7805
PRIMER_LEFT_1_PENALTY=0.575240
PRIMER_RIGHT_1_PENALTY=0.205226
PRIMER_LEFT_1_SEQUENCE=GAGAGTGTGACCAAGTGTTGCT
PRIMER_RIGHT_1_SEQUENCE=ACCCAACTCTGAAGAACAAAGC
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=287,22
PRIMER_LEFT_1_TM=59.425
PRIMER_RIGHT_1_TM=59.795
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.8298
PRIMER_LEFT_2_PENALTY=0.575240
PRIMER_RIGHT_2_PENALTY=0.254567
PRIMER_LEFT_2_SEQUENCE=GAGAGTGTGACCAAGTGTTGCT
PRIMER_RIGHT_2_SEQUENCE=TAGAAGAGTTGGGAGAGGTTGG
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=250,22
PRIMER_LEFT_2_TM=59.425
PRIMER_RIGHT_2_TM=59.745
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.8658
PRIMER_LEFT_3_PENALTY=0.575240
PRIMER_RIGHT_3_PENALTY=0.290589
PRIMER_LEFT_3_SEQUENCE=GAGAGTGTGACCAAGTGTTGCT
PRIMER_RIGHT_3_SEQUENCE=TGACTCAGAGGAATTGACGTTG
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=179,22
PRIMER_LEFT_3_TM=59.425
PRIMER_RIGHT_3_TM=60.291
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=178
PRIMER_PAIR_PENALTY_4=0.8772
PRIMER_LEFT_4_PENALTY=0.575240
PRIMER_RIGHT_4_PENALTY=0.301926
PRIMER_LEFT_4_SEQUENCE=GAGAGTGTGACCAAGTGTTGCT
PRIMER_RIGHT_4_SEQUENCE=GAGGTTGGTGGAGACTTAGCTG
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=236,22
PRIMER_LEFT_4_TM=59.425
PRIMER_RIGHT_4_TM=60.302
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_05242 Ps_contig_mira-and-tgicl-ass_franssen_36926
SEQUENCE=CGCCTGAAGAATGAAAAGAGAGAGAGCAAGGAATTGAGAGTGGAAGAGATTTAGAATGAACTTCAGAAATTGGAAGAATGAATCCCCTTGAAAATGGCAGGGAGGTATGTTTATATAGGATTGCAGAGTTGGTTAGAGATTGCGATGAAATTCACAGGAAATGCAAGGTTAGTTATCAAATGCATAATTCGGTTAGAGTGAAATGAGAATGAATCTGAGTTAGTGTGAATTCAGTTCAGTTAGTCGGTTGTTAGCAATCCTGTTATGACTCTATTAGATTCTATTTTTTAAGTTTGTTAAATGTTAAGTTCTGTTGAAATCTCTGTCAGTTGGTGGTTAAGTTAACTTGAATGCTAGTTGGATTTTCCAGTTAAGGATGAATGATTATTATGTTGAAGTTCGGTCGAAGTCAGTTATCTACAAATTATGTGTAAAAATTGTTAGTTATGCATTGTTACTTTGTGACAATAAGATGTTAATGAGTTAGATGGATTGAAGCTAATGATGCCCTTGCCATTGGTTTGTTTTTGATTTGCTGCAAATGAATCATGTTGCTTGAGTTATTCTGAATACAAAATTAGTTAATATGAACTTAAAAAAAACG
TARGET=17,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05243 Ps_contig_mira-and-tgicl-ass_franssen_36928
SEQUENCE=CGCGGGGATAACCAACAAAACAAAACCACTCTCTCTCTTTCTCTCACAAAAAAAATGTTTTCTTTTTCTTCAATATCTAAAGAGTAAAAGCAAGTCCAAAATTGTTGCTTGTTGAAAACCCTTGAAGGAAAGTACAAAACAATCAAACCCCATATTGAAAGTTTTCATTTTTAAAACTTTAATATAGTATAGTAGTGATGAAGTGGAAGTAAGAGAGAGAGAGAGAGCGAGAGGGGGGAGAGAGAGAGATTCATATGAAGAGTAACGAGTATTGTAGGAAGAAAG
TARGET=29,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.0734
PRIMER_LEFT_PENALTY=2.587455
PRIMER_RIGHT_PENALTY=0.485895
PRIMER_LEFT_SEQUENCE=CGGGGATAACCAACAAAACA
PRIMER_RIGHT_SEQUENCE=TCCTTCAAGGGTTTTCAACAAG
PRIMER_LEFT=2,20
PRIMER_RIGHT=128,22
PRIMER_LEFT_TM=60.587
PRIMER_RIGHT_TM=60.486
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=127
PRIMER_PAIR_PENALTY_1=3.2902
PRIMER_LEFT_1_PENALTY=2.804263
PRIMER_RIGHT_1_PENALTY=0.485895
PRIMER_LEFT_1_SEQUENCE=CGGGGATAACCAACAAAACAA
PRIMER_RIGHT_1_SEQUENCE=TCCTTCAAGGGTTTTCAACAAG
PRIMER_LEFT_1=2,21
PRIMER_RIGHT_1=128,22
PRIMER_LEFT_1_TM=61.804
PRIMER_RIGHT_1_TM=60.486
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=3.3632
PRIMER_LEFT_2_PENALTY=2.587455
PRIMER_RIGHT_2_PENALTY=0.775744
PRIMER_LEFT_2_SEQUENCE=CGGGGATAACCAACAAAACA
PRIMER_RIGHT_2_SEQUENCE=CCTCTCGCTCTCTCTCTCTCTC
PRIMER_LEFT_2=2,20
PRIMER_RIGHT_2=233,22
PRIMER_LEFT_2_TM=60.587
PRIMER_RIGHT_2_TM=59.224
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=59.091
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=3.3887
PRIMER_LEFT_3_PENALTY=2.902852
PRIMER_RIGHT_3_PENALTY=0.485895
PRIMER_LEFT_3_SEQUENCE=CGGGGATAACCAACAAAACAAA
PRIMER_RIGHT_3_SEQUENCE=TCCTTCAAGGGTTTTCAACAAG
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=128,22
PRIMER_LEFT_3_TM=62.903
PRIMER_RIGHT_3_TM=60.486
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=3.4356
PRIMER_LEFT_4_PENALTY=2.587455
PRIMER_RIGHT_4_PENALTY=0.848146
PRIMER_LEFT_4_SEQUENCE=CGGGGATAACCAACAAAACA
PRIMER_RIGHT_4_SEQUENCE=CTTTCCTTCAAGGGTTTTCAAC
PRIMER_LEFT_4=2,20
PRIMER_RIGHT_4=131,22
PRIMER_LEFT_4_TM=60.587
PRIMER_RIGHT_4_TM=59.152
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_05244 Ps_contig_mira-and-tgicl-ass_franssen_36928
SEQUENCE=CGCGGGGATAACCAACAAAACAAAACCACTCTCTCTCTTTCTCTCACAAAAAAAATGTTTTCTTTTTCTTCAATATCTAAAGAGTAAAAGCAAGTCCAAAATTGTTGCTTGTTGAAAACCCTTGAAGGAAAGTACAAAACAATCAAACCCCATATTGAAAGTTTTCATTTTTAAAACTTTAATATAGTATAGTAGTGATGAAGTGGAAGTAAGAGAGAGAGAGAGAGCGAGAGGGGGGAGAGAGAGAGATTCATATGAAGAGTAACGAGTATTGTAGGAAGAAAG
TARGET=212,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0899
PRIMER_LEFT_PENALTY=0.848146
PRIMER_RIGHT_PENALTY=1.241766
PRIMER_LEFT_SEQUENCE=GTTGAAAACCCTTGAAGGAAAG
PRIMER_RIGHT_SEQUENCE=TATGAATCTCTCTCTCTCCCCC
PRIMER_LEFT=110,22
PRIMER_RIGHT=254,22
PRIMER_LEFT_TM=59.152
PRIMER_RIGHT_TM=58.758
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=12.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=2.5914
PRIMER_LEFT_1_PENALTY=1.349668
PRIMER_RIGHT_1_PENALTY=1.241766
PRIMER_LEFT_1_SEQUENCE=CCAACAAAACAAAACCACTCTC
PRIMER_RIGHT_1_SEQUENCE=TATGAATCTCTCTCTCTCCCCC
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=254,22
PRIMER_LEFT_1_TM=58.650
PRIMER_RIGHT_1_TM=58.758
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=12.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=2.6014
PRIMER_LEFT_2_PENALTY=1.359656
PRIMER_RIGHT_2_PENALTY=1.241766
PRIMER_LEFT_2_SEQUENCE=GTTGCTTGTTGAAAACCCTTG
PRIMER_RIGHT_2_SEQUENCE=TATGAATCTCTCTCTCTCCCCC
PRIMER_LEFT_2=103,21
PRIMER_RIGHT_2=254,22
PRIMER_LEFT_2_TM=59.640
PRIMER_RIGHT_2_TM=58.758
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=12.4000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=2.6291
PRIMER_LEFT_3_PENALTY=1.387329
PRIMER_RIGHT_3_PENALTY=1.241766
PRIMER_LEFT_3_SEQUENCE=GTTGCTTGTTGAAAACCCTTGA
PRIMER_RIGHT_3_SEQUENCE=TATGAATCTCTCTCTCTCCCCC
PRIMER_LEFT_3=103,22
PRIMER_RIGHT_3=254,22
PRIMER_LEFT_3_TM=61.387
PRIMER_RIGHT_3_TM=58.758
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=12.4000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=2.7731
PRIMER_LEFT_4_PENALTY=0.848146
PRIMER_RIGHT_4_PENALTY=1.924975
PRIMER_LEFT_4_SEQUENCE=GTTGAAAACCCTTGAAGGAAAG
PRIMER_RIGHT_4_SEQUENCE=ATATGAATCTCTCTCTCTCCCCC
PRIMER_LEFT_4=110,22
PRIMER_RIGHT_4=255,23
PRIMER_LEFT_4_TM=59.152
PRIMER_RIGHT_4_TM=59.075
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=12.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_05245 Ps_contig_mira-and-tgicl-ass_franssen_36928
SEQUENCE=CGCGGGGATAACCAACAAAACAAAACCACTCTCTCTCTTTCTCTCACAAAAAAAATGTTTTCTTTTTCTTCAATATCTAAAGAGTAAAAGCAAGTCCAAAATTGTTGCTTGTTGAAAACCCTTGAAGGAAAGTACAAAACAATCAAACCCCATATTGAAAGTTTTCATTTTTAAAACTTTAATATAGTATAGTAGTGATGAAGTGGAAGTAAGAGAGAGAGAGAGAGCGAGAGGGGGGAGAGAGAGAGATTCATATGAAGAGTAACGAGTATTGTAGGAAGAAAG
TARGET=238,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05246 Ps_contig_mira-and-tgicl-ass_franssen_36929
SEQUENCE=GGGGGTGGAAAAACCGATAGCCATTNAGATAACCTCTGTTATCGTCGTTCATTCACGCCTCACTCCACCAATGGCTTCTTCTCTCCACCAAAACCCCACCTTCCTTAAACCCCACCACCACCCTCAATCCCCAAAACCCACCACCACCACACGCCGGAGCTACATCCCGGTACAGTGCGGAGGACCGCGTTCCCAGCGTGGACCACTAGTAAAAGGCCGTTTCCTAAGCATCGAAGCAATCCAAGCCATCCAAACCCTAAAACGCGTCCAACGAACCAACCCACCGAACCAAACTCAACTCCTAACCAACACGCTAACCCGCTTAATCAAATCCGACTTAGTCGCAACGTTAAAGGAGCTTCTACGCCAACAACAATGCACGCTCGCACTCCGTGTCTTCTCTGCCGTTAGATCTGAATACGGAGCAAATCTTTCTCTCTACGCGGAAATCGTGCAGGCACTCGGCAATTCCGGTATGTCGGAAGATGTGGACCGTTTGATTAACGAGATTGAGGATGAAGGTGGGATTAGAGTTGATGAGGATCAGAGGAAAGGGCTTTTGTATTTGATTAAAAGTGTTATCGGAGCAAAGAGAAGAGATTCGATGATTAGGGTTTATGAGATGATGAAGAGAAGTGGGTGGGTTCNGNTGTNACTGNTGATTATATTTGGTTTAAAGGTTTTGAATTTAAGGTTGGGGTTTTTAAGGG
TARGET=138,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4520
PRIMER_LEFT_PENALTY=0.418908
PRIMER_RIGHT_PENALTY=0.033137
PRIMER_LEFT_SEQUENCE=GGAAAAACCGATAGCCATTNAG
PRIMER_RIGHT_SEQUENCE=TAGGAGTTGAGTTTGGTTCGGT
PRIMER_LEFT=6,22
PRIMER_RIGHT=303,22
PRIMER_LEFT_TM=60.419
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6500
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=0.6558
PRIMER_LEFT_1_PENALTY=0.418908
PRIMER_RIGHT_1_PENALTY=0.236882
PRIMER_LEFT_1_SEQUENCE=GGAAAAACCGATAGCCATTNAG
PRIMER_RIGHT_1_SEQUENCE=ATTGCTTCGATGCTTAGGAAAC
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=239,22
PRIMER_LEFT_1_TM=60.419
PRIMER_RIGHT_1_TM=59.763
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6500
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.7076
PRIMER_LEFT_2_PENALTY=0.418908
PRIMER_RIGHT_2_PENALTY=0.288644
PRIMER_LEFT_2_SEQUENCE=GGAAAAACCGATAGCCATTNAG
PRIMER_RIGHT_2_SEQUENCE=CCTTTTACTAGTGGTCCACGCT
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=215,22
PRIMER_LEFT_2_TM=60.419
PRIMER_RIGHT_2_TM=59.711
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6500
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.9244
PRIMER_LEFT_3_PENALTY=0.418908
PRIMER_RIGHT_3_PENALTY=0.505477
PRIMER_LEFT_3_SEQUENCE=GGAAAAACCGATAGCCATTNAG
PRIMER_RIGHT_3_SEQUENCE=TTAGGAGTTGAGTTTGGTTCGG
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=304,22
PRIMER_LEFT_3_TM=60.419
PRIMER_RIGHT_3_TM=60.505
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6500
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.9516
PRIMER_LEFT_4_PENALTY=0.918479
PRIMER_RIGHT_4_PENALTY=0.033137
PRIMER_LEFT_4_SEQUENCE=CAAAACCCCACCTTCCTTAAAC
PRIMER_RIGHT_4_SEQUENCE=TAGGAGTTGAGTTTGGTTCGGT
PRIMER_LEFT_4=88,22
PRIMER_RIGHT_4=303,22
PRIMER_LEFT_4_TM=60.918
PRIMER_RIGHT_4_TM=60.033
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_05247 Ps_contig_mira-and-tgicl-ass_franssen_36938
SEQUENCE=ACTTTTATTCAAGTCATTAGCAGTTCAAAGAAAGCTCTTACACTTGAAAAATTGAATAGCTATAGATTATTAAACTGAATAGAAAATTGAAGTTACACATATGAAACCATTTATATTAATTATAACAAAAAGAGAATGACTATTTAACTTAAAATCACCATAATAAAAAAAATGCTGAAATTAATTATGAACTTCTCAGTCTTGATCACGATCCATGCAAGATCATAGTTGAGTTGCATTGAGAGAACTTGAATTCATCATTTGATCATAGTTGTAATTGATTTGTCTTGTATCCATCTCCATTGCACCAACATGTGAAGGTAAATATTGTTGTGTAGACAAAGACAGTGATGATGATGATGGAAAAGGTACAACATTAATTCCATCTATATCTTTAATTTGGTTAACAAATCCAAAATTTGATAATTCATCATGAGGAACATGATTAGATGAAGGCTCCAATTGGTAACCAAGTGGCATAGGATTCAATAATTCTAAGAAAGAAGGGTGATTTGCTCTTTGTAAGAGATTATTTCTTTGAGAAATTTCTCTTATATTTCCTCCATTAGAAGTAATCATATTTGAAGTACGCTCAGACACCAAATCTGAAAAGAGGTTATTTGGCAAAATATATTGTGTGTGATAACAATCTTCAGACTCCACTTTTTTCTCTTTCTCTTTTTCGCTTCTATCTATCCATTTTTCTTTGTTAGTAACGATTTCTCCTAACCCTTGTCTTGAATTTGATTTCCAAATAGAAGTATTGTTTTGGAAAACATATTCATTAGAAATAGTACTAGCAAAAGATGGATAACCTAGGGTGAAATTTCCTTGAGGTATTGGAAGTGGTGGAAGTTCATCAATTTCATCCTTAGCTATGTTAAGCAACCAATCAACAACTTTGCTAGGTTGATTTAACCCTAATCTATCTTGAAGATCATAAAGATGAATTGCTGTTTGAATTGAAAGTCTTACCCTTCTATCTCTTAGTCCTTTTATTGTGTATACTTTGCTATGTCTATCTTTTGTTCCAAAGGCTCTTGATACTCTCACAATTCTTGGATCCTTTAGTTTTAACCATTGTGATGTTGATGATGATGATGGAGTAGTTGAATCCTTTGATGGAAGAACTTCTTGTTTTAATGGAATTTCAATCATCTTTTGTCTCTCATTGTTGTTATTGTTGGGCTCCTCCAAGTGCAAAGTTTTGTTGTTTTGGTTGATATTCTCTCTAATTTTGAATTCTTCTTTATGAATTCTCACTTCTAGTATCTTGCTTTTGATGCTATATATGATATGAAGATGGTTTGAAAAATGAAAGATGAGTTTGGTTTGATTTTATAGAATGAAAATGGTGAACAAGTTATTATAAGTTTGAGTTTGCAGGTAGAGGAAGATGCTGGAATCCCCGAC
TARGET=349,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3258
PRIMER_LEFT_PENALTY=0.291669
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=ATTGCACCAACATGTGAAGGTA
PRIMER_RIGHT_SEQUENCE=ACCAATTGGAGCCTTCATCTAA
PRIMER_LEFT=301,22
PRIMER_RIGHT=466,22
PRIMER_LEFT_TM=60.292
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.3258
PRIMER_LEFT_1_PENALTY=0.291669
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=ATTGCACCAACATGTGAAGGTA
PRIMER_RIGHT_1_SEQUENCE=TTACCAATTGGAGCCTTCATCT
PRIMER_LEFT_1=301,22
PRIMER_RIGHT_1=468,22
PRIMER_LEFT_1_TM=60.292
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=168
PRIMER_PAIR_PENALTY_2=0.4504
PRIMER_LEFT_2_PENALTY=0.291669
PRIMER_RIGHT_2_PENALTY=0.158697
PRIMER_LEFT_2_SEQUENCE=ATTGCACCAACATGTGAAGGTA
PRIMER_RIGHT_2_SEQUENCE=GTTACCAATTGGAGCCTTCATC
PRIMER_LEFT_2=301,22
PRIMER_RIGHT_2=469,22
PRIMER_LEFT_2_TM=60.292
PRIMER_RIGHT_2_TM=59.841
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.5294
PRIMER_LEFT_3_PENALTY=0.291669
PRIMER_RIGHT_3_PENALTY=0.237699
PRIMER_LEFT_3_SEQUENCE=ATTGCACCAACATGTGAAGGTA
PRIMER_RIGHT_3_SEQUENCE=CAAAGAGCAAATCACCCTTCTT
PRIMER_LEFT_3=301,22
PRIMER_RIGHT_3=521,22
PRIMER_LEFT_3_TM=60.292
PRIMER_RIGHT_3_TM=59.762
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.5608
PRIMER_LEFT_4_PENALTY=0.291669
PRIMER_RIGHT_4_PENALTY=0.269153
PRIMER_LEFT_4_SEQUENCE=ATTGCACCAACATGTGAAGGTA
PRIMER_RIGHT_4_SEQUENCE=ATTGAATCCTATGCCACTTGGT
PRIMER_LEFT_4=301,22
PRIMER_RIGHT_4=489,22
PRIMER_LEFT_4_TM=60.292
PRIMER_RIGHT_4_TM=59.731
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_05248 Ps_contig_mira-and-tgicl-ass_franssen_36938
SEQUENCE=ACTTTTATTCAAGTCATTAGCAGTTCAAAGAAAGCTCTTACACTTGAAAAATTGAATAGCTATAGATTATTAAACTGAATAGAAAATTGAAGTTACACATATGAAACCATTTATATTAATTATAACAAAAAGAGAATGACTATTTAACTTAAAATCACCATAATAAAAAAAATGCTGAAATTAATTATGAACTTCTCAGTCTTGATCACGATCCATGCAAGATCATAGTTGAGTTGCATTGAGAGAACTTGAATTCATCATTTGATCATAGTTGTAATTGATTTGTCTTGTATCCATCTCCATTGCACCAACATGTGAAGGTAAATATTGTTGTGTAGACAAAGACAGTGATGATGATGATGGAAAAGGTACAACATTAATTCCATCTATATCTTTAATTTGGTTAACAAATCCAAAATTTGATAATTCATCATGAGGAACATGATTAGATGAAGGCTCCAATTGGTAACCAAGTGGCATAGGATTCAATAATTCTAAGAAAGAAGGGTGATTTGCTCTTTGTAAGAGATTATTTCTTTGAGAAATTTCTCTTATATTTCCTCCATTAGAAGTAATCATATTTGAAGTACGCTCAGACACCAAATCTGAAAAGAGGTTATTTGGCAAAATATATTGTGTGTGATAACAATCTTCAGACTCCACTTTTTTCTCTTTCTCTTTTTCGCTTCTATCTATCCATTTTTCTTTGTTAGTAACGATTTCTCCTAACCCTTGTCTTGAATTTGATTTCCAAATAGAAGTATTGTTTTGGAAAACATATTCATTAGAAATAGTACTAGCAAAAGATGGATAACCTAGGGTGAAATTTCCTTGAGGTATTGGAAGTGGTGGAAGTTCATCAATTTCATCCTTAGCTATGTTAAGCAACCAATCAACAACTTTGCTAGGTTGATTTAACCCTAATCTATCTTGAAGATCATAAAGATGAATTGCTGTTTGAATTGAAAGTCTTACCCTTCTATCTCTTAGTCCTTTTATTGTGTATACTTTGCTATGTCTATCTTTTGTTCCAAAGGCTCTTGATACTCTCACAATTCTTGGATCCTTTAGTTTTAACCATTGTGATGTTGATGATGATGATGGAGTAGTTGAATCCTTTGATGGAAGAACTTCTTGTTTTAATGGAATTTCAATCATCTTTTGTCTCTCATTGTTGTTATTGTTGGGCTCCTCCAAGTGCAAAGTTTTGTTGTTTTGGTTGATATTCTCTCTAATTTTGAATTCTTCTTTATGAATTCTCACTTCTAGTATCTTGCTTTTGATGCTATATATGATATGAAGATGGTTTGAAAAATGAAAGATGAGTTTGGTTTGATTTTATAGAATGAAAATGGTGAACAAGTTATTATAAGTTTGAGTTTGCAGGTAGAGGAAGATGCTGGAATCCCCGAC
TARGET=1090,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3401
PRIMER_LEFT_PENALTY=1.224301
PRIMER_RIGHT_PENALTY=0.115769
PRIMER_LEFT_SEQUENCE=TGTTCCAAAGGCTCTTGATACTC
PRIMER_RIGHT_SEQUENCE=GGAGCCCAACAATAACAACAAT
PRIMER_LEFT=1026,23
PRIMER_RIGHT=1191,22
PRIMER_LEFT_TM=59.776
PRIMER_RIGHT_TM=60.116
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=1.4019
PRIMER_LEFT_1_PENALTY=1.224301
PRIMER_RIGHT_1_PENALTY=0.177596
PRIMER_LEFT_1_SEQUENCE=TGTTCCAAAGGCTCTTGATACTC
PRIMER_RIGHT_1_SEQUENCE=CAACAAAACTTTGCACTTGGAG
PRIMER_LEFT_1=1026,23
PRIMER_RIGHT_1=1212,22
PRIMER_LEFT_1_TM=59.776
PRIMER_RIGHT_1_TM=59.822
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=1.6915
PRIMER_LEFT_2_PENALTY=1.575769
PRIMER_RIGHT_2_PENALTY=0.115769
PRIMER_LEFT_2_SEQUENCE=CCAAAGGCTCTTGATACTCTCAC
PRIMER_RIGHT_2_SEQUENCE=GGAGCCCAACAATAACAACAAT
PRIMER_LEFT_2=1030,23
PRIMER_RIGHT_2=1191,22
PRIMER_LEFT_2_TM=59.424
PRIMER_RIGHT_2_TM=60.116
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=1.7534
PRIMER_LEFT_3_PENALTY=1.575769
PRIMER_RIGHT_3_PENALTY=0.177596
PRIMER_LEFT_3_SEQUENCE=CCAAAGGCTCTTGATACTCTCAC
PRIMER_RIGHT_3_SEQUENCE=CAACAAAACTTTGCACTTGGAG
PRIMER_LEFT_3=1030,23
PRIMER_RIGHT_3=1212,22
PRIMER_LEFT_3_TM=59.424
PRIMER_RIGHT_3_TM=59.822
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=1.7687
PRIMER_LEFT_4_PENALTY=1.224301
PRIMER_RIGHT_4_PENALTY=0.544368
PRIMER_LEFT_4_SEQUENCE=TGTTCCAAAGGCTCTTGATACTC
PRIMER_RIGHT_4_SEQUENCE=AACAAAACTTTGCACTTGGAGG
PRIMER_LEFT_4=1026,23
PRIMER_RIGHT_4=1211,22
PRIMER_LEFT_4_TM=59.776
PRIMER_RIGHT_4_TM=60.544
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_05249 Ps_contig_mira-and-tgicl-ass_franssen_36940
SEQUENCE=CTAAAAATAAAAACAAAAAACCANNAATAACTCTAAATACAAAAACCAACTTAAAACCTACCACTCCTCCAAAAACCCTACCCTTCCACCAGGTCCACCAGCAGCTCCAGCACCACGGCCGAACGGAATTGGTTGAACAGCGACCGGATCGACCTGCACAACACCGGGAATCTCCGACATCCCAAGAGCAGCAAGCATATCAAGCGTCTCCTTATCAACCTCAATGTGATCAGTCCTGATAGCAGACACATCAGGAACGAAATCCATACGCCTCTCACGCTCCTCCTCTTGTAGCTTCAACGAGATTCCACGAACAGGTCCCTTCTGAATCCTCTTCATCAAATGAGTTGAAAACCCAGCGATTTTGTTGCGAAGCCTCTTGGTTGGAATGAGTGCGACTTCCTCGAGGATTTTCTTGTTGGTGTGGAAATCCAAGGTCATTCGAGAGTAGTAACGCTCGATGACTTGTCTCGAGGATTTCTTCACGGTTTTGGTTCTGACACGACCCATTTTGTGACGGTGAGTGTGTGTGTGTGGTGTGGGTGAAGGAAGAGACGAAGAAGTAAAGTTTAGGTTTTCGTCGGAAGGAAGGAGGCACCACCCCGCGTACTCTGTCGTTGACTACC
TARGET=524,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5725
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=0.498031
PRIMER_LEFT_SEQUENCE=GAAATCCAAGGTCATTCGAGAG
PRIMER_RIGHT_SEQUENCE=CTTCTTCGTCTCTTCCTTCACC
PRIMER_LEFT=426,22
PRIMER_RIGHT=562,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.502
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=0.5727
PRIMER_LEFT_1_PENALTY=0.074695
PRIMER_RIGHT_1_PENALTY=0.498031
PRIMER_LEFT_1_SEQUENCE=AGGTCCCTTCTGAATCCTCTTC
PRIMER_RIGHT_1_SEQUENCE=CTTCTTCGTCTCTTCCTTCACC
PRIMER_LEFT_1=315,22
PRIMER_RIGHT_1=562,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=59.502
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.6153
PRIMER_LEFT_2_PENALTY=0.117281
PRIMER_RIGHT_2_PENALTY=0.498031
PRIMER_LEFT_2_SEQUENCE=TTGTAGCTTCAACGAGATTCCA
PRIMER_RIGHT_2_SEQUENCE=CTTCTTCGTCTCTTCCTTCACC
PRIMER_LEFT_2=288,22
PRIMER_RIGHT_2=562,22
PRIMER_LEFT_2_TM=59.883
PRIMER_RIGHT_2_TM=59.502
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=275
PRIMER_PAIR_PENALTY_3=0.8214
PRIMER_LEFT_3_PENALTY=0.323355
PRIMER_RIGHT_3_PENALTY=0.498031
PRIMER_LEFT_3_SEQUENCE=CGAGAGTAGTAACGCTCGATGA
PRIMER_RIGHT_3_SEQUENCE=CTTCTTCGTCTCTTCCTTCACC
PRIMER_LEFT_3=442,22
PRIMER_RIGHT_3=562,22
PRIMER_LEFT_3_TM=59.677
PRIMER_RIGHT_3_TM=59.502
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=121
PRIMER_PAIR_PENALTY_4=0.8468
PRIMER_LEFT_4_PENALTY=0.348730
PRIMER_RIGHT_4_PENALTY=0.498031
PRIMER_LEFT_4_SEQUENCE=TCCCTTCTGAATCCTCTTCATC
PRIMER_RIGHT_4_SEQUENCE=CTTCTTCGTCTCTTCCTTCACC
PRIMER_LEFT_4=318,22
PRIMER_RIGHT_4=562,22
PRIMER_LEFT_4_TM=59.651
PRIMER_RIGHT_4_TM=59.502
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_05250 Ps_contig_mira-and-tgicl-ass_franssen_36941
SEQUENCE=TTGTTGTTGTTGGAGAAGATGTGTTTGTGATGGCTCATATGAAGAGGTGGAAAAAGGAGTGTGTGTTCTTCAAGAAAGCGAGGAAACATTTTTCCATTGATGTTTTGCATGTTGATACTCCTTGTAATGGTTCTAGAGTTGGTGTTGTTGTTTATCGTTTTGTTGGAAAGGGTTAATTGACTAGCACGGTTTAAAGTTACATTTGTTTGGTTGTTATTTTCTAATCAATCGAGTTTTTTAGGATATTGATGTTTTTTTTGTTTGCTTTCCAAATGAAGCATGTTCAAGTTCAGCCTTTTCT
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05251 Ps_contig_mira-and-tgicl-ass_franssen_36943
SEQUENCE=TTGTAAGGTATAAAACATTCTAGCTTCCCTTTTCTATTCATATTCCTTTAGTTTGCCCTCGGAAACAATAATGGTTCACATTAAGAGATATAGAGTTCACAGACATAGAGGTACATAAAATGAATACTGTTATACAGCAACCAAATGACAACACACTTGTTCTATTGAAAAATCCATAGCTGTATTACGAAAGAGTTAAACAAACAAAACACAAACCACTCAAACAAAAGAGGCAACACTCTTCCCAACTTATAGATATAGATAATTATTCTTCAAAGTCATTGCGAGTGCCTTCAATTGTACTCAATAGGGCAATATCTTCCACTGCTGGTTATCACCACTGTTCCAGTCCCAGAGGACAACAGTAGTGCCATCATGTACACCTCCAGAATCTTTATCACCATGAAAAGCATCCATGTTGAGATGAATATTGTTGACCATCCTTACAGCTCGGTAGCCGCTACCCTGGTCCTTGCTCTCAGTCCATAAAACAGTCTGATCAAGATAGTCCGGATTGTAACGTGCTAGCCGAACTGGATGGGTGGCACCAATGGAATGCTTAATGGCCTCACCAGTGACCTTGTTGACCAAAGCAAAAGAAGGGTAACCATCTTTATCCTTCACCCTGGTGCTCCACTTCTCATCCTTGTACCAATGCTGATGCTGATCTGAGGGATCGGAGGGTGCGAGAACCACTTCGCCGCGACGGATAGTGAGAGAGTGATTCGGGGAAGCTTTGGTGAGGATCTTGAAAGTAGGTTTGTTGGAGGAGATAGGATGACCGATGGGTGACACCTCATGGCTGACGTGGTGAACGGTGGTTGGAAAATGAGGGTGATGTGATTCGTGAGAAACGTGATGAACAGTAGCGTTAGGGAATGGGGTGGAGGAGTGATCAGGTATGGATGGGGGTGCAGGTGGGTATCCACCGTAGTTATGAGGTTGAGGCGGAGGTGGAGCAGGGTAACTGAAGTCGTCACGGGACACGTGTCCAGTGTGGAAGACTTGAGTTTCTGGCTGGTGAGGTAGTTGTGGTGGTGGGTATGAGTCGTTGTAGAATGGCGGTGGTGGTTGATGATGATGATGCGGCGGCGGTGGTTGGTTGTAGGGGGAGGAGGTGTGGCCTGGTGGAGGGAATTGTGGTTCTTCGGTGTCGTCTCTTGGGTTGTGGTGATGAGTGTGGGTTACGTTTGAGGAGTTACGGCCATGGTTGAATGGGAAATCCATTTTGCGATTATTTTGATTGTTGTTGTTGCTACTGGAGTTGAAGTTGGAGTTAGAGTTGGAATTGGAATTAATAGAAAAGAGATGGATCTTCTGTCGTTANGCTTAGTTTTGTTGTTAT
TARGET=1073,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1482
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.061818
PRIMER_LEFT_SEQUENCE=TGGTGGGTATGAGTCGTTGTAG
PRIMER_RIGHT_SEQUENCE=GCCGTAACTCCTCAAACGTAAC
PRIMER_LEFT=1035,22
PRIMER_RIGHT=1204,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=60.062
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.1589
PRIMER_LEFT_1_PENALTY=0.086339
PRIMER_RIGHT_1_PENALTY=0.072552
PRIMER_LEFT_1_SEQUENCE=TGGTGGGTATGAGTCGTTGTAG
PRIMER_RIGHT_1_SEQUENCE=CGTAACTCCTCAAACGTAACCC
PRIMER_LEFT_1=1035,22
PRIMER_RIGHT_1=1202,22
PRIMER_LEFT_1_TM=59.914
PRIMER_RIGHT_1_TM=59.927
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=168
PRIMER_PAIR_PENALTY_2=0.1589
PRIMER_LEFT_2_PENALTY=0.086339
PRIMER_RIGHT_2_PENALTY=0.072552
PRIMER_LEFT_2_SEQUENCE=TGGTGGGTATGAGTCGTTGTAG
PRIMER_RIGHT_2_SEQUENCE=CCGTAACTCCTCAAACGTAACC
PRIMER_LEFT_2=1035,22
PRIMER_RIGHT_2=1203,22
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=59.927
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.2137
PRIMER_LEFT_3_PENALTY=0.151911
PRIMER_RIGHT_3_PENALTY=0.061818
PRIMER_LEFT_3_SEQUENCE=TATGAGTCGTTGTAGAATGGCG
PRIMER_RIGHT_3_SEQUENCE=GCCGTAACTCCTCAAACGTAAC
PRIMER_LEFT_3=1042,22
PRIMER_RIGHT_3=1204,22
PRIMER_LEFT_3_TM=60.152
PRIMER_RIGHT_3_TM=60.062
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=163
PRIMER_PAIR_PENALTY_4=0.2245
PRIMER_LEFT_4_PENALTY=0.151911
PRIMER_RIGHT_4_PENALTY=0.072552
PRIMER_LEFT_4_SEQUENCE=TATGAGTCGTTGTAGAATGGCG
PRIMER_RIGHT_4_SEQUENCE=CGTAACTCCTCAAACGTAACCC
PRIMER_LEFT_4=1042,22
PRIMER_RIGHT_4=1202,22
PRIMER_LEFT_4_TM=60.152
PRIMER_RIGHT_4_TM=59.927
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_05252 Ps_contig_mira-and-tgicl-ass_franssen_36943
SEQUENCE=TTGTAAGGTATAAAACATTCTAGCTTCCCTTTTCTATTCATATTCCTTTAGTTTGCCCTCGGAAACAATAATGGTTCACATTAAGAGATATAGAGTTCACAGACATAGAGGTACATAAAATGAATACTGTTATACAGCAACCAAATGACAACACACTTGTTCTATTGAAAAATCCATAGCTGTATTACGAAAGAGTTAAACAAACAAAACACAAACCACTCAAACAAAAGAGGCAACACTCTTCCCAACTTATAGATATAGATAATTATTCTTCAAAGTCATTGCGAGTGCCTTCAATTGTACTCAATAGGGCAATATCTTCCACTGCTGGTTATCACCACTGTTCCAGTCCCAGAGGACAACAGTAGTGCCATCATGTACACCTCCAGAATCTTTATCACCATGAAAAGCATCCATGTTGAGATGAATATTGTTGACCATCCTTACAGCTCGGTAGCCGCTACCCTGGTCCTTGCTCTCAGTCCATAAAACAGTCTGATCAAGATAGTCCGGATTGTAACGTGCTAGCCGAACTGGATGGGTGGCACCAATGGAATGCTTAATGGCCTCACCAGTGACCTTGTTGACCAAAGCAAAAGAAGGGTAACCATCTTTATCCTTCACCCTGGTGCTCCACTTCTCATCCTTGTACCAATGCTGATGCTGATCTGAGGGATCGGAGGGTGCGAGAACCACTTCGCCGCGACGGATAGTGAGAGAGTGATTCGGGGAAGCTTTGGTGAGGATCTTGAAAGTAGGTTTGTTGGAGGAGATAGGATGACCGATGGGTGACACCTCATGGCTGACGTGGTGAACGGTGGTTGGAAAATGAGGGTGATGTGATTCGTGAGAAACGTGATGAACAGTAGCGTTAGGGAATGGGGTGGAGGAGTGATCAGGTATGGATGGGGGTGCAGGTGGGTATCCACCGTAGTTATGAGGTTGAGGCGGAGGTGGAGCAGGGTAACTGAAGTCGTCACGGGACACGTGTCCAGTGTGGAAGACTTGAGTTTCTGGCTGGTGAGGTAGTTGTGGTGGTGGGTATGAGTCGTTGTAGAATGGCGGTGGTGGTTGATGATGATGATGCGGCGGCGGTGGTTGGTTGTAGGGGGAGGAGGTGTGGCCTGGTGGAGGGAATTGTGGTTCTTCGGTGTCGTCTCTTGGGTTGTGGTGATGAGTGTGGGTTACGTTTGAGGAGTTACGGCCATGGTTGAATGGGAAATCCATTTTGCGATTATTTTGATTGTTGTTGTTGCTACTGGAGTTGAAGTTGGAGTTAGAGTTGGAATTGGAATTAATAGAAAAGAGATGGATCTTCTGTCGTTANGCTTAGTTTTGTTGTTAT
TARGET=1244,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4839
PRIMER_LEFT_PENALTY=0.061818
PRIMER_RIGHT_PENALTY=0.422071
PRIMER_LEFT_SEQUENCE=GTTACGTTTGAGGAGTTACGGC
PRIMER_RIGHT_SEQUENCE=AAGCNTAACGACAGAAGATCCA
PRIMER_LEFT=1183,22
PRIMER_RIGHT=1330,22
PRIMER_LEFT_TM=60.062
PRIMER_RIGHT_TM=59.578
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.1000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.75
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=0.4946
PRIMER_LEFT_1_PENALTY=0.072552
PRIMER_RIGHT_1_PENALTY=0.422071
PRIMER_LEFT_1_SEQUENCE=GGTTACGTTTGAGGAGTTACGG
PRIMER_RIGHT_1_SEQUENCE=AAGCNTAACGACAGAAGATCCA
PRIMER_LEFT_1=1182,22
PRIMER_RIGHT_1=1330,22
PRIMER_LEFT_1_TM=59.927
PRIMER_RIGHT_1_TM=59.578
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.75
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.4946
PRIMER_LEFT_2_PENALTY=0.072552
PRIMER_RIGHT_2_PENALTY=0.422071
PRIMER_LEFT_2_SEQUENCE=GGGTTACGTTTGAGGAGTTACG
PRIMER_RIGHT_2_SEQUENCE=AAGCNTAACGACAGAAGATCCA
PRIMER_LEFT_2=1181,22
PRIMER_RIGHT_2=1330,22
PRIMER_LEFT_2_TM=59.927
PRIMER_RIGHT_2_TM=59.578
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.75
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=0.5084
PRIMER_LEFT_3_PENALTY=0.086339
PRIMER_RIGHT_3_PENALTY=0.422071
PRIMER_LEFT_3_SEQUENCE=TGGTGGGTATGAGTCGTTGTAG
PRIMER_RIGHT_3_SEQUENCE=AAGCNTAACGACAGAAGATCCA
PRIMER_LEFT_3=1035,22
PRIMER_RIGHT_3=1330,22
PRIMER_LEFT_3_TM=59.914
PRIMER_RIGHT_3_TM=59.578
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=296
PRIMER_PAIR_PENALTY_4=0.5242
PRIMER_LEFT_4_PENALTY=0.102129
PRIMER_RIGHT_4_PENALTY=0.422071
PRIMER_LEFT_4_SEQUENCE=GATGAGTGTGGGTTACGTTTGA
PRIMER_RIGHT_4_SEQUENCE=AAGCNTAACGACAGAAGATCCA
PRIMER_LEFT_4=1172,22
PRIMER_RIGHT_4=1330,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=59.578
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.75
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=159
=
PRIMER_SEQUENCE_ID=SSR_05253 Ps_contig_mira-and-tgicl-ass_franssen_36944
SEQUENCE=CGGGGTTATTTCCTNTNNNGNGCTCCGCATCACATTCTCTTTTAAGTCTCTCATACCTAACCAACCACTAACAACATCAACAACAACAAGAAAATGGGTACAGCTACCTGCATCGATATCATCGTTGCCATCCTCCTTCCACCTCTCGGCGTCTTCCTCAAATTTGGCTGCAAAGTTGAGTTCTGGCTCTGTTTGGTGCTCACCCTTTTAGGTTATCTTCCTGGAATCCTCTATGCCATCTATGCTATCACTAAATGATCCATCATCTTCTATGTGGATCGTGAATAGTTTTCAGGGGCGTCATTGCTGTATTTCAATTTAGATATGTATTAGTTTCGTGTAAAAGTTGTTCACTCATGATGGACAAAATTTATTCTTCTTTTATTTTATAACAATAATTTCCGTGTCAATAGAGTTCTTCTCTTGTTTTGCTGTTTTTGACTTCGGTTTTTGTCTTATGTGAAAAATGATACTATGTGAAGCTTGTGAACAGTCGATTTGAACTACTATATATTAATAAATTTGTTATCTTATTGACNAATTAACG
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8036
PRIMER_LEFT_PENALTY=0.638917
PRIMER_RIGHT_PENALTY=0.164639
PRIMER_LEFT_SEQUENCE=CTCCGCATCACATTCTCTTTTA
PRIMER_RIGHT_SEQUENCE=CCCTGAAAACTATTCACGATCC
PRIMER_LEFT=22,22
PRIMER_RIGHT=296,22
PRIMER_LEFT_TM=59.361
PRIMER_RIGHT_TM=59.835
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=1.5515
PRIMER_LEFT_1_PENALTY=0.638917
PRIMER_RIGHT_1_PENALTY=0.912566
PRIMER_LEFT_1_SEQUENCE=CTCCGCATCACATTCTCTTTTA
PRIMER_RIGHT_1_SEQUENCE=CCTGAAAACTATTCACGATCCA
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=295,22
PRIMER_LEFT_1_TM=59.361
PRIMER_RIGHT_1_TM=59.087
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=274
PRIMER_PAIR_PENALTY_2=1.6674
PRIMER_LEFT_2_PENALTY=0.638917
PRIMER_RIGHT_2_PENALTY=1.028502
PRIMER_LEFT_2_SEQUENCE=CTCCGCATCACATTCTCTTTTA
PRIMER_RIGHT_2_SEQUENCE=CAAACAGAGCCAGAACTCAACTT
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=194,23
PRIMER_LEFT_2_TM=59.361
PRIMER_RIGHT_2_TM=59.971
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=1.6792
PRIMER_LEFT_3_PENALTY=0.638917
PRIMER_RIGHT_3_PENALTY=1.040302
PRIMER_LEFT_3_SEQUENCE=CTCCGCATCACATTCTCTTTTA
PRIMER_RIGHT_3_SEQUENCE=AATGACGCCCCTGAAAACTATT
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=304,22
PRIMER_LEFT_3_TM=59.361
PRIMER_RIGHT_3_TM=61.040
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=1.7399
PRIMER_LEFT_4_PENALTY=0.638917
PRIMER_RIGHT_4_PENALTY=1.100969
PRIMER_LEFT_4_SEQUENCE=CTCCGCATCACATTCTCTTTTA
PRIMER_RIGHT_4_SEQUENCE=ACCAAACAGAGCCAGAACTCA
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=196,21
PRIMER_LEFT_4_TM=59.361
PRIMER_RIGHT_4_TM=59.899
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_05254 Ps_contig_mira-and-tgicl-ass_franssen_36953
SEQUENCE=CCGCTATCATGACCTCCGCAACACAAATTAACAATATGAAGGCTCCTATTACAACAGATTTAGGATCAATTGCCACACCTTATGACTATGGCGCCGGGGAAATAACATCAACAGAACCGTTCCAACCCGGGCTAGTTTATGAAACTAATACCTTGGACTACTTGAACTACTTATGTTACATAGGATTCAACACAACCACAATTAAGGTTATCTCCAGAACTGTCCCAGATAGTTTCAGTTGTCCCAAGGATTCTACTTCTGGTCATATTTCCAATATAAACTATCCTTCCATAGCAATCTCCAACTTCAATGGTAAAGATATCGTGAATGTGAGTAGAACTGTGACGAATGTTGGTGAAGAAGACGAAACGGTCTACTCCGCCATTGTTGATGCTCCTAGTGGAGTGAAAGTCCAGTTGGTTCCGGAGAAACTCCAATTTACAAAAAGCAGCAAACAACTAAGCTATCAAGTTATTTTCTCCTCTAGTTTAAGCTCCTTGAAGAATGATCTGTTTGGATCTATTACATGGAGTAATGGTAAACATAATGTTCGAAGTCCCTTTGTATTAAGTATGTAAGGTAAATAAATAAATAAAATTAATAAATACAAGAAGAGTTCTTGTACATGTATTCTACCATCCTTANATAGATAAACTTA
TARGET=581,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05255 Ps_contig_mira-and-tgicl-ass_franssen_36959
SEQUENCE=CTTCATTCAACATATAAATGGCAATTCGTAGAAAAATAAGCTATGAGATCACATGATCGATTGATATAATTTTGCTAGCTAAGATCCATAACATTCATGTGAGAACTGAAAACAACTGTCTAGATTATGCCGTCAAGAAATTACAATTGTTCTCTCACTAACTCATTTAACCATTCATTCAAAAAGTTCAAAAACATGTGTTCTAACTAGGTGAAAACTGACCGCAGACAGAATATCACGCTCTAGCCTCCGATGTCTGATTGCAAAGGTACTAAATTGTCGAAGATTAACGCTTCTTCTTGCCTCCTTTACCGCCTGATTTTGATGGAGTGGCTACCAGGTACACAAACATAACGATAATGCTGATCACCGAGATTAGAGATCCATACTTAGCCAGCAACCTCTTAGCCCATTCAAACTTCTTCTCCGGGGGCCTGTCAGCAAGAACATCTAATGGCAATATGGGAGTTGAAAGTGCCTCCTGTAAAGCAGCATTTGTGGGAACACGGAACTTGATGACAGCTGGTTCACCAGAAAATACTCCCTTTGATTTTGCCTCCAGCTCAAATGTGTGAGAGAGGACACCACCAGCATCAAGCTTTTCCCATGTCTTTGAAGTGGTACCGCTGATTATACTGAAAAGGTCCGCTGGCCAGCTCTCATCTGCCAGACTCACATCATATGCCGTCGAGGTTCCTTGGTTGTAGATATCGATGGTGACAGAGACTCTTTCAGCGCCAGTCTTGAGCCTGTTGAGAGAAGCCTTCTTGTGAGCGACGATGAAGGGAACGTCAGAGGAAGCTTGTGAGCACAGCAGCAGAGAAGCTATGAGCAGAAACGCAATCAGAGGCTTGGAGATTGGATCCGCCATTGGCACAGAGAGAGAGAGAGAGAGAGAGAGAGATGAAGAGTCAATGAAATCGTGTCTCCNNNCGTACTCTGCGT
TARGET=878,26
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2344
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.232060
PRIMER_LEFT_SEQUENCE=TCGAGGTTCCTTGGTTGTAGAT
PRIMER_RIGHT_SEQUENCE=NGGAGACACGATTTCATTGACT
PRIMER_LEFT=687,22
PRIMER_RIGHT=930,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.768
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.2712
PRIMER_LEFT_1_PENALTY=0.039178
PRIMER_RIGHT_1_PENALTY=0.232060
PRIMER_LEFT_1_SEQUENCE=CTATGAGCAGAAACGCAATCAG
PRIMER_RIGHT_1_SEQUENCE=NGGAGACACGATTTCATTGACT
PRIMER_LEFT_1=825,22
PRIMER_RIGHT_1=930,22
PRIMER_LEFT_1_TM=60.039
PRIMER_RIGHT_1_TM=59.768
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=0.2914
PRIMER_LEFT_2_PENALTY=0.059316
PRIMER_RIGHT_2_PENALTY=0.232060
PRIMER_LEFT_2_SEQUENCE=CAGCAGAGAAGCTATGAGCAGA
PRIMER_RIGHT_2_SEQUENCE=NGGAGACACGATTTCATTGACT
PRIMER_LEFT_2=814,22
PRIMER_RIGHT_2=930,22
PRIMER_LEFT_2_TM=60.059
PRIMER_RIGHT_2_TM=59.768
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=0.4461
PRIMER_LEFT_3_PENALTY=0.214036
PRIMER_RIGHT_3_PENALTY=0.232060
PRIMER_LEFT_3_SEQUENCE=CAGTCTTGAGCCTGTTGAGAGA
PRIMER_RIGHT_3_SEQUENCE=NGGAGACACGATTTCATTGACT
PRIMER_LEFT_3=738,22
PRIMER_RIGHT_3=930,22
PRIMER_LEFT_3_TM=59.786
PRIMER_RIGHT_3_TM=59.768
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.6422
PRIMER_LEFT_4_PENALTY=0.410097
PRIMER_RIGHT_4_PENALTY=0.232060
PRIMER_LEFT_4_SEQUENCE=AAGCTATGAGCAGAAACGCAAT
PRIMER_RIGHT_4_SEQUENCE=NGGAGACACGATTTCATTGACT
PRIMER_LEFT_4=822,22
PRIMER_RIGHT_4=930,22
PRIMER_LEFT_4_TM=60.410
PRIMER_RIGHT_4_TM=59.768
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_05256 Ps_contig_mira-and-tgicl-ass_franssen_36962
SEQUENCE=CATCAACAGAATCAACAATATCAGCAGCAGCAGCAACAACAACAGTGGCTGAGAAGGAACCAGTTGAGTGGAACTGACACCAGTGTTGTGGAGGAGGTCGAGAAGACTGTGCAGTCTGAAACAAACGACTCTAGTTCACAAGATTGGAAGGCAAGACTAAAGATTCCACCAGCTGATACACGCTATAGGACAGAGGATGTGACAGCAACTAAAGGAAATGAGTTTGAGGATTACTTTTTGAAGCGTGAGCTGCTAATGGGAATATATGAGAAGGGGTTTGAAAGGCCATCCCCTATCCAAGAAGAAAGCATTCCAATTGCCCTTA
TARGET=22,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05257 Ps_contig_mira-and-tgicl-ass_franssen_36963
SEQUENCE=CTGCTCTTGATTATACTATTTGCCTCCATAGTGAAGCATTTGTTACAACTCAGGGTGGAAATTTTCCTCACTTCTTGATGGGTCACAGGAGATATATGTATGGTGGGCATGCAAAGACAATTAAACCCGAAAAGAGAAAATTGGTTGTATTATTTGATAACTCTGATGTAAGATGGGAAGTATTTAAGCAACAAATGAAAGACATGCTTAACCATAGTGATCAAAGGGGAACCGAAGTAAAAAAGACTGGTGAATCCCTATATACTTTTCCCATGCCAGATTGCATGTCTAAACAGGTAGAACCCAAATATGAAAATGCTAACACTGGGAAAAATATGATATTATAACTGTAAAACAGTTAATTGTCTTTAGGAAATCAAATTGTTCAATAGATGTAATTTATATATATATATCATTAAGTTTGAATTCAAAAAGGATAGTTTGATTCAAACTTGAAGAAAAGCCATATACTAAGGCTTCAATTTCTTCTTTATTTTCTGATTTGTTAGTAATAGTTAGTTAGGTGTGAAATTATAATTCTTTGTACATGAGATTGTTGTAACCCAAGATAGACTTCCACTTGATTATGAAGATTCAATTTTGGAGGAGTGGCAGCTGCTGATTAACCAACGATAAAAGAATAATAACAATTGGTTGATATTGTTGCTCAGATGATTCATTTTCCTCATGTCATGTGCAGAAATAGTTTAAGAAATTATTTATTTTTGTGTTGTAACAATAACTAGTCATTGTAAGAAACCAGTTTGGATGGAGGCTTATTTTTATTATTTA
TARGET=401,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=5.0862
PRIMER_LEFT_PENALTY=1.918416
PRIMER_RIGHT_PENALTY=3.167799
PRIMER_LEFT_SEQUENCE=GCATGTCTAAACAGGTAGAACCC
PRIMER_RIGHT_SEQUENCE=GGAAGTCTATCTTGGGTTACAACAA
PRIMER_LEFT=282,23
PRIMER_RIGHT=577,25
PRIMER_LEFT_TM=59.082
PRIMER_RIGHT_TM=59.832
PRIMER_LEFT_GC_PERCENT=47.826
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=5.5655
PRIMER_LEFT_1_PENALTY=2.397662
PRIMER_RIGHT_1_PENALTY=3.167799
PRIMER_LEFT_1_SEQUENCE=TTGCATGTCTAAACAGGTAGAACC
PRIMER_RIGHT_1_SEQUENCE=GGAAGTCTATCTTGGGTTACAACAA
PRIMER_LEFT_1=280,24
PRIMER_RIGHT_1=577,25
PRIMER_LEFT_1_TM=59.602
PRIMER_RIGHT_1_TM=59.832
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=5.7667
PRIMER_LEFT_2_PENALTY=2.397662
PRIMER_RIGHT_2_PENALTY=3.368993
PRIMER_LEFT_2_SEQUENCE=TTGCATGTCTAAACAGGTAGAACC
PRIMER_RIGHT_2_SEQUENCE=GTGGAAGTCTATCTTGGGTTACAA
PRIMER_LEFT_2=280,24
PRIMER_RIGHT_2=579,24
PRIMER_LEFT_2_TM=59.602
PRIMER_RIGHT_2_TM=58.631
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=5.7667
PRIMER_LEFT_3_PENALTY=2.397662
PRIMER_RIGHT_3_PENALTY=3.368993
PRIMER_LEFT_3_SEQUENCE=TTGCATGTCTAAACAGGTAGAACC
PRIMER_RIGHT_3_SEQUENCE=GGAAGTCTATCTTGGGTTACAACA
PRIMER_LEFT_3=280,24
PRIMER_RIGHT_3=577,24
PRIMER_LEFT_3_TM=59.602
PRIMER_RIGHT_3_TM=58.631
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=5.8415
PRIMER_LEFT_4_PENALTY=2.673725
PRIMER_RIGHT_4_PENALTY=3.167799
PRIMER_LEFT_4_SEQUENCE=TGCATGTCTAAACAGGTAGAACC
PRIMER_RIGHT_4_SEQUENCE=GGAAGTCTATCTTGGGTTACAACAA
PRIMER_LEFT_4=281,23
PRIMER_RIGHT_4=577,25
PRIMER_LEFT_4_TM=58.326
PRIMER_RIGHT_4_TM=59.832
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_05258 Ps_contig_mira-and-tgicl-ass_franssen_36965
SEQUENCE=AGTACGCGGNGGTGGAGGAAGGAGTACAATACAAAGTAATAAACAAGTTTGTTTTTCTCTCTCTTTCTTTTTCTCTAAGATTTGTTGAATTGGTGGTGGTAGGAAAATAGGAAGAAATGAGAAAGACGAAGAAAAGTAGCAGTACTACGTCAGATTCAGAGTATTGTCAACAACAACAACCAGGTTTTACTGCTGCGGCGGAAGAACAAACAACTTCTCCTCCTCCTAAGCTTGACGATGGTTTCTATGAAATTGAAAGGATTCGCCGTAAGAGGATCAGAAAGGGTGAGCTCCAGTACCTTATTAAATGGCGAGGATGGCCGGAAACTGCCAACACCTGGGAGCCTCTTCAAAATCTACAATCTGTTCCCGATCTCATTCATGCATTTGAAGAAAGCCTCAAATCAGGCGGTGCTGGAGGAGGCGGAAATCATCATCGCAGCAAGGGAAAACGCACCACCACCCTTCCACCTCACCCTCAGCCACCACAACCTACTAACGCCCATCCTCCCACCGTCCTTCAAAATCCACCATCTACGGACACTGGCCTCCATGCAGACAACCTAGCAACACATTTCCAACAGACTGTCATTTCAACGCCCAATGATGCTCAATCGAACGGGAACGGGGATCCGCTTCAAACGGACCGCACCAGAGGAGCCAAAAGGAGAAAACCTGCTTCTGTTAAAAGGTTCAAGAAAGAGGAATGTGCATCTGAAACTGTTGTTGATACAAACAATGCAGCTGCCAATGACGTCTCTGCTGGTGGTAAGCTTGAACCGGCCTGGACACAAAATGCATGCAATATTGTCAAGATTCTAAAGCCAATAGGCTATTCAGCTTCTCTATCTTCCTACATGCAGGATGTTTCAGTCACCTTTATGGCTATGAGGTCTGATGGAACAGAAGTGATGGTGGATAACAAATATCTCAAGACAAACAATCCTCTTCTGCTGATCAATTTCTATGAGCAGCATCTCCGATACAATCCAAGTTCTTAACTGGATAGAGTATTATTGAAGGTGGTGGTTTTTTGTGTGATTAGATCTTCTTTTGTTTGTTGCTAGTTATATATAATGGAGTTGTAGATGCAGGGAAATACGATGTTGGTTAGTATATGATTATATAACCAAATCTTTGGAGACTTGCTTTAAACTAGTGATTCTACATTATGTTTTATCCCCTTTACGCATGATAACAGTCTGTAGATGTATCTTTGGTTGTGCATTGAACATCATTGAAATTTGGTGTGTTAATATTGTTTCACTAAAGGCGTTGTTTAANGAAGGCTGCATATATGATTGCTTAANAACG
TARGET=168,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2254
PRIMER_LEFT_PENALTY=0.102139
PRIMER_RIGHT_PENALTY=0.123278
PRIMER_LEFT_SEQUENCE=TTGGTGGTGGTAGGAAAATAGG
PRIMER_RIGHT_SEQUENCE=CACCCTTTCTGATCCTCTTACG
PRIMER_LEFT=89,22
PRIMER_RIGHT=287,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=60.123
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=7.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.2461
PRIMER_LEFT_1_PENALTY=0.102139
PRIMER_RIGHT_1_PENALTY=0.143968
PRIMER_LEFT_1_SEQUENCE=TTGGTGGTGGTAGGAAAATAGG
PRIMER_RIGHT_1_SEQUENCE=GGAGAAGTTGTTTGTTCTTCCG
PRIMER_LEFT_1=89,22
PRIMER_RIGHT_1=219,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.2517
PRIMER_LEFT_2_PENALTY=0.102139
PRIMER_RIGHT_2_PENALTY=0.149516
PRIMER_LEFT_2_SEQUENCE=TTGGTGGTGGTAGGAAAATAGG
PRIMER_RIGHT_2_SEQUENCE=TAGATTTTGAAGAGGCTCCCAG
PRIMER_LEFT_2=89,22
PRIMER_RIGHT_2=358,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=59.850
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=270
PRIMER_PAIR_PENALTY_3=0.3075
PRIMER_LEFT_3_PENALTY=0.102139
PRIMER_RIGHT_3_PENALTY=0.205314
PRIMER_LEFT_3_SEQUENCE=TTGGTGGTGGTAGGAAAATAGG
PRIMER_RIGHT_3_SEQUENCE=ATGAATGAGATCGGGAACAGAT
PRIMER_LEFT_3=89,22
PRIMER_RIGHT_3=382,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=59.795
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=0.3605
PRIMER_LEFT_4_PENALTY=0.237199
PRIMER_RIGHT_4_PENALTY=0.123278
PRIMER_LEFT_4_SEQUENCE=TTTGTTGAATTGGTGGTGGTAG
PRIMER_RIGHT_4_SEQUENCE=CACCCTTTCTGATCCTCTTACG
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=287,22
PRIMER_LEFT_4_TM=59.763
PRIMER_RIGHT_4_TM=60.123
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_05259 Ps_contig_mira-and-tgicl-ass_franssen_36968
SEQUENCE=CCTTCAAATCTTGCCAGGAAGCAGAAGATGGGAGTCCACGAACAATAACTCGAAATTCAGAATGGCGTGAGACACCAAATCGGCCTCCACCACCACCACCACTGTTACGGTTTTGAGCTTCGTTAACGGTAACGTTACGCCCGTCCATGTTCTGGCCGTTCATCCCTTCAATAGCATCTCTCATCGATTTCTCATTAGCGAATGTCAC
TARGET=87,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6605
PRIMER_LEFT_PENALTY=0.116582
PRIMER_RIGHT_PENALTY=0.543880
PRIMER_LEFT_SEQUENCE=ATGGGAGTCCACGAACAATAAC
PRIMER_RIGHT_SEQUENCE=CGCTAATGAGAAATCGATGAGA
PRIMER_LEFT=27,22
PRIMER_RIGHT=199,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=59.456
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=0.6605
PRIMER_LEFT_1_PENALTY=0.116582
PRIMER_RIGHT_1_PENALTY=0.543880
PRIMER_LEFT_1_SEQUENCE=ATGGGAGTCCACGAACAATAAC
PRIMER_RIGHT_1_SEQUENCE=TCGCTAATGAGAAATCGATGAG
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=200,22
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=59.456
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=174
PRIMER_PAIR_PENALTY_2=0.6737
PRIMER_LEFT_2_PENALTY=0.129818
PRIMER_RIGHT_2_PENALTY=0.543880
PRIMER_LEFT_2_SEQUENCE=GGGAGTCCACGAACAATAACTC
PRIMER_RIGHT_2_SEQUENCE=CGCTAATGAGAAATCGATGAGA
PRIMER_LEFT_2=29,22
PRIMER_RIGHT_2=199,22
PRIMER_LEFT_2_TM=59.870
PRIMER_RIGHT_2_TM=59.456
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.6737
PRIMER_LEFT_3_PENALTY=0.129818
PRIMER_RIGHT_3_PENALTY=0.543880
PRIMER_LEFT_3_SEQUENCE=GGGAGTCCACGAACAATAACTC
PRIMER_RIGHT_3_SEQUENCE=TCGCTAATGAGAAATCGATGAG
PRIMER_LEFT_3=29,22
PRIMER_RIGHT_3=200,22
PRIMER_LEFT_3_TM=59.870
PRIMER_RIGHT_3_TM=59.456
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.9376
PRIMER_LEFT_4_PENALTY=0.116582
PRIMER_RIGHT_4_PENALTY=0.821019
PRIMER_LEFT_4_SEQUENCE=ATGGGAGTCCACGAACAATAAC
PRIMER_RIGHT_4_SEQUENCE=GATGCTATTGAAGGGATGAACG
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=177,22
PRIMER_LEFT_4_TM=60.117
PRIMER_RIGHT_4_TM=60.821
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_05260 Ps_contig_mira-and-tgicl-ass_franssen_36971
SEQUENCE=ATAATCCACGTAGTCAAAATTGTAAGGCCAAAATTCTAGGATGAAATTCCNACTAAAACGTGTCCAATCTTTCTTCAATTCAATTCAATTTCTCTCATTTTTCACACCATGCCTCTTCTTCCTCCCACTGATTACGCTACGTTTCTCGTAATCACACAATTCAGTTCAATACTCCACAATCCCAATTTCAATGTTGGTTTGTAAGATTATTCATATTCATTGTCACGACCCTATGTTCTTTCACTTCTCATCAATCCATCCATGATTCTTCAACAAGCTTCTCCCACTTTTCACTTCTCTCTTTGTCCCTGCAACAACAATGCTTTTCCTTCTAATTTTCCCCTTTCCAAAACCATGAAGAAACGCTTCATTTCAAATTATTACTCGTATCACAAATTCCG
TARGET=74,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8032
PRIMER_LEFT_PENALTY=0.725159
PRIMER_RIGHT_PENALTY=0.078029
PRIMER_LEFT_SEQUENCE=TCCNACTAAAACGTGTCCAATC
PRIMER_RIGHT_SEQUENCE=AAGTGGGAGAAGCTTGTTGAAG
PRIMER_LEFT=47,22
PRIMER_RIGHT=288,22
PRIMER_LEFT_TM=59.275
PRIMER_RIGHT_TM=59.922
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.75
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.8315
PRIMER_LEFT_1_PENALTY=0.725159
PRIMER_RIGHT_1_PENALTY=0.106310
PRIMER_LEFT_1_SEQUENCE=TCCNACTAAAACGTGTCCAATC
PRIMER_RIGHT_1_SEQUENCE=TCATGGATGGATTGATGAGAAG
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=264,22
PRIMER_LEFT_1_TM=59.275
PRIMER_RIGHT_1_TM=59.894
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=218
PRIMER_PAIR_PENALTY_2=0.8660
PRIMER_LEFT_2_PENALTY=0.725159
PRIMER_RIGHT_2_PENALTY=0.140832
PRIMER_LEFT_2_SEQUENCE=TCCNACTAAAACGTGTCCAATC
PRIMER_RIGHT_2_SEQUENCE=GAACATAGGGTCGTGACAATGA
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=237,22
PRIMER_LEFT_2_TM=59.275
PRIMER_RIGHT_2_TM=59.859
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.8936
PRIMER_LEFT_3_PENALTY=0.815523
PRIMER_RIGHT_3_PENALTY=0.078029
PRIMER_LEFT_3_SEQUENCE=CGTAGTCAAAATTGTAAGGCCA
PRIMER_RIGHT_3_SEQUENCE=AAGTGGGAGAAGCTTGTTGAAG
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=288,22
PRIMER_LEFT_3_TM=59.184
PRIMER_RIGHT_3_TM=59.922
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=281
PRIMER_PAIR_PENALTY_4=0.9218
PRIMER_LEFT_4_PENALTY=0.815523
PRIMER_RIGHT_4_PENALTY=0.106310
PRIMER_LEFT_4_SEQUENCE=CGTAGTCAAAATTGTAAGGCCA
PRIMER_RIGHT_4_SEQUENCE=TCATGGATGGATTGATGAGAAG
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=264,22
PRIMER_LEFT_4_TM=59.184
PRIMER_RIGHT_4_TM=59.894
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_05261 Ps_contig_mira-and-tgicl-ass_franssen_36977
SEQUENCE=AAAANCCAAAATATTAACTTATCAATTATCACTACTTTTACTAAACATTGTCTCTAGATAGAAATGTGCAGTTTATTATTTCAGAAGAAAAGTAATGAATTTGATATTAACAGAAATCCATTGATAGGAGAAAATACTAAACAAACTTCAACATTCAAATTAAGTTAATAACCAACATAAACAGCAGCATTCACAATGTAGCAATCCTCATTCTTGTCACCCATCTCTTCCACAAGCACCCCACTTGAGCATATCAAACCACCATCCACAACTTCAACATTCAAAATTTTCCCATAGGGAAATACAGACTTGACTTTGTCAATATCTAGAGATTGCTGGAGAGAATGAGGAACCCCTAACTTGATCTGCAATTTCATCTCACCGAAACTAACACCAGGAATGGAACCTCTGCGAAAAGCGGGGATTGAATTAGAAGAAATAGCATCCCTGCAGGCTCTCATTGCTGCAGAAGTTATATTCTGCCCGTGTTGATCGTAGCCGACACCCATTTCCACAAACAAGAGTTTCATTGGTTGGGATGAATCGGAAGATCCTTGAGTTTGTTCAATTTCCATGTGCGGGATTGTAGCTTGGGTTTTGCGAGAAATTTGACAGGGTTGAAAGTTTGATAATCGATGGTGTGAGAAAGAAAGAGATTTGACGGGGGRAGTAGTGATGAGTGAAATGTGTTTGGTAAAACATGGAATTTGAAGATTTAGAAGTGAACGACATTCAGTCATTTCTTCACGGTCTCTTTCTNTCCYCCCTCGTTACNAAACCTTGTAATTAAGGACTTATTATTATTATT
TARGET=795,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05262 Ps_contig_mira-and-tgicl-ass_franssen_36989
SEQUENCE=AAATTACATATCACAAGTCACAACAGTTTTAGTTATTACCATTTTCTACAATCTTTCTAGTATTATGCATTGATATAGGAAAACCATCTTCTTTATTATCTTATCAACTTTTTCCTAGCATTAGNAGCCATCCAAATTATTCATTCAAGATCACTAAACAAATTACACTACAAAGGTTAGCCATTATCAAGCTGAAAAAATATGAATTTTAAGCCAATTGTCTGCAAGGGCAGCACAGCCACAAGCTGGTATACAATTTATACCACAATCAAATACCCAAAAATTGATGGTAACAAAAAAAATTGATAGCTCACAGGTGATGATGGTCCTCAAATTGGCACGTAGCGTTTCAACGCCTGCTTCGCCGTGACAGCCACTCCGACCACAACTCCACCAACAGCACCTGCTACAGCAGCTGATCTAACCCCTCTCGCAGCTCGATACAACACGCCAGTGCCAAGCCCTGCTGCTATGCTGTTCAACACATCGTCGGTATCTCTCGCGGCCGTGATCCCGCTCTCAATGCCAGCGTAAAGCAACCCGATCACACCGAGCCGGTTACCCCACGTCCGACCGGTGTGACCTGACGAGTTGAGAATCCGATTGACTCTGAGCTTCATGGTATCTCCTGACTCGAACGATTTAACGCCTTCGACGAGACCTACGCCGGCGCCGGCAACGGCTCCGCCGAGGTAGCCGCAGCCGGTGAGGAAAGTTAGGGTTTCTCCCCAGGAGCGGTGCTTTCGGGCAGCCTCCTCGACGAAAAGGTGCTCGGGTGAGGTTGGGAGCTGGTAGAGGTTTCGAATGGGTACTTCGAGGTCTTTGTAGGGATTGTAGAGTCGTTTTTGGTTATTTGAATCGGAACCTGATTCGTGATTGGAGGTCTGGTACGCCATCGGAGAGACGCTTCCAGACTCTAGGGTTAGGGTTTGGGCGGAGAGAGAGAGAGATGCCGAAGAACGACGCTGTTTGTGTGGNACCCCGAC
TARGET=937,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3517
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=1.349375
PRIMER_LEFT_SEQUENCE=TCGAGGTCTTTGTAGGGATTGT
PRIMER_RIGHT_SEQUENCE=ACACAAACAGCGTCGTTCTTC
PRIMER_LEFT=813,22
PRIMER_RIGHT=974,21
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.349
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=162
PRIMER_PAIR_PENALTY_1=1.4780
PRIMER_LEFT_1_PENALTY=0.128591
PRIMER_RIGHT_1_PENALTY=1.349375
PRIMER_LEFT_1_SEQUENCE=GGGATTGTAGAGTCGTTTTTGG
PRIMER_RIGHT_1_SEQUENCE=ACACAAACAGCGTCGTTCTTC
PRIMER_LEFT_1=827,22
PRIMER_RIGHT_1=974,21
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=60.349
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=148
PRIMER_PAIR_PENALTY_2=1.5332
PRIMER_LEFT_2_PENALTY=0.183834
PRIMER_RIGHT_2_PENALTY=1.349375
PRIMER_LEFT_2_SEQUENCE=GGTTATTTGAATCGGAACCTGA
PRIMER_RIGHT_2_SEQUENCE=ACACAAACAGCGTCGTTCTTC
PRIMER_LEFT_2=847,22
PRIMER_RIGHT_2=974,21
PRIMER_LEFT_2_TM=60.184
PRIMER_RIGHT_2_TM=60.349
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=128
PRIMER_PAIR_PENALTY_3=1.6114
PRIMER_LEFT_3_PENALTY=0.261998
PRIMER_RIGHT_3_PENALTY=1.349375
PRIMER_LEFT_3_SEQUENCE=GAGCTGGTAGAGGTTTCGAATG
PRIMER_RIGHT_3_SEQUENCE=ACACAAACAGCGTCGTTCTTC
PRIMER_LEFT_3=785,22
PRIMER_RIGHT_3=974,21
PRIMER_LEFT_3_TM=60.262
PRIMER_RIGHT_3_TM=60.349
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=190
PRIMER_PAIR_PENALTY_4=1.6827
PRIMER_LEFT_4_PENALTY=0.333282
PRIMER_RIGHT_4_PENALTY=1.349375
PRIMER_LEFT_4_SEQUENCE=GAAAGTTAGGGTTTCTCCCCAG
PRIMER_RIGHT_4_SEQUENCE=ACACAAACAGCGTCGTTCTTC
PRIMER_LEFT_4=710,22
PRIMER_RIGHT_4=974,21
PRIMER_LEFT_4_TM=60.333
PRIMER_RIGHT_4_TM=60.349
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_05263 Ps_contig_mira-and-tgicl-ass_franssen_36991
SEQUENCE=GTCGGGGTAAAATTAAAAATAATATTTTGGATCNRRGTGGGGAAGATGATGAATCGGAATAGCAGAGACACACTTCGTTTCCTCTCACGCTTCCATCATCACCAACAACAAAACCCATCTCTTCTACCTTATGCATCACCGTCTTCTCAATTCACATCAGTAACAGGTTTTCATGAAAATTCCCCACAATTACAGCGCAATGTTGTTCCCGGGGAACATAAAGCTTTGTCTTTTTTCAATCCGCTGAGAAATTCCGTGAAAAATGAGCAATTGGGTGGTGGAAACCCTTTCGAAATTCATCAGAAATGTTGTTATGGTTCAATGGGTGGAGTGGTTCAGAGGAGCTCCAGATTCTCAGAGTTGAATGATGATGATGTTAAATACTTTCAAGAGATTTTGGGGAAGAAAAATGTTGTACAGGATGAAGATAAGCTCAGTGCTGCTAATATTGATTGGATGCATAAGTATAAAGGCTCCAGTAAGATTCTTCTACAGCCTTGCAACACTGATCAGGTTTCTGAGATTCTTAAATACTGTAACTCCAGAAACCTGGCCGTTGTTCCTCAAGGTGGAAACACTGGTCTTGTAGGTGGAAGTGTGCCCGTCTTTGATGAAGTGATTGTTAGTCTTAGTTCAATGAACAAGATCGTATCTTTTGACAAGGTTAGTGGAATACTGGTATGTGAAGCTGGGTGCATATTGGAAAATCTAATGTCATTCATGGACAATGAAGGATTTATTATGCCCCTAGACTTAGGTGCTAAAGGGAGTTGCCAGATTGGTGGAAATGTTTCAACTAATGCCGGTGGTTTGCGTCTTGTTCGTTAATAGGANYNCTNNNTTAACATGGAAGTGTACTTGGTGTTGAAGCT
TARGET=365,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05264 Ps_contig_mira-and-tgicl-ass_franssen_36997
SEQUENCE=GTTCGGGGTNACTACCTAAAAANTCTATTTCTAAACAAAAACTTTGTAAAAATCACAACCATTGAAGAAACAATCAATCTTCCATAGAAGAGATTCAATTGGTAAAGAAAGTTTCACTTTTTTTTTAGGAAAGCCAAGGATCTGAGTAGTAGTGAAAAGAGAGAGAAAAGATGTGTGGTGGTGCTATTATCTCCGACTTCATCCCTGCTACGGCGGCTGTAGGTGGTTCCCGCCGTGTTACTGCTGATATCCTGTGGCCGAATTTGAGGAAAAACGGTTTGAAGAAACCGTTTTTGCTTGACGATGATTTCGAGGCTGGGTTCAGAGAGTTCAAGGATGATTCTGATTTTGATGAGGATGAGGATGAGGAGGAAGATGAAGGGTTGATGCTCGGTGTGAAAGGGTTTTCTTTTTCTTCCGGCAACAACAAGTCTTCAAGGACCTTCTCTCGTGGATCGGGTGCTGCAAAGCCTGTGGTGTCGAAATCGAATGAGCAACCTGAAAAGGATTCGAAGAGGAAGAGGAAGAATCAGTATAGGGGTATCCGACAACGTCCGTGGGGGAAATGGGCAGCTGAGATTCGTGACCCGAGGAAAGGAGTTCGTGTTTGGCTTGGAACGTTCAACACTGCTGAAGAAGCTGCAAGAGCTTATGATGCTGAAGCTAGGAGAATCCGTGGCTCGAAAGCCAAGGTGAATTTCCCTGAGGAGACTCCAAATGCTTCTGCAAAACGTGTTAAGCCGAGTCCTGAGGCGCAGCTGGTGAACAAGAATCTGAACTCGTTCAAACCGAATGGGAATGCGATGTTCAACTTCAGTGAGAATATGGAGACCTACTATTCCCCTATGGATCAGGTTGAACAGAAGCCGATGGTTAACAACCAGTTTGCGAACATGGGAGTGTTTGCGGGAAATGGATTTCAGCTTGCAGCTGCTGATGTTAATGCTTACTTCAGCTCAGAGCATTCGAGCAACTCGTTTGATTATTCTGATCTAGGTTGGGGCGAACAAGCCCCGAAAACCCCTGAGATTACGTCTGTGTTTTCCGCTCCTGCTCCCCGGGAAGGCGAATCTCAGATCAATCTGCAATCTGCCAATGCTCAGGATATGCTGCCTATGCAAGATGATTCTGCAAAGACACTCTCCGAGGAGCTTGCTGATATCGAGTCCCAGCTGAAGTTCTTCGAGAACTCGTTTGATGATAACTGGAGTGATGCTTCATTGGCGTCTTTGCTCGGTACTGATTCAGTTCAGGATGGTGGAAACACAATGAGCCTCTGGAGCTTTGATGACATGCCCGCCATCTCCGGCGGAGTTTTCTGAACAACGTCCGCTCCGCCTCGCCGATTATGTAAATAAAGCTACAAGATTGTTGGGTTATTTATATTGTTGATGGCATCAAGAAGAAGCACAAGCTTAAGCAAGTTTGGAGTTTTATTCTCTTGTGGCGAATAAGTTATTCATAGTTTTTTTTAATTAGGTATCCTTTTGTCTTGTCAAAAACCTTTTGAGGATTTTTTTCAGTTTGATGTTTATAAGGATTATGTTATGTTATGTTTATGGATGTTATAATTGTAAACTTGGTTGTTTTTGCATTATGACAATTTTCTCCCTATGTTTGGTGGGATGAATTTATATCATGTGAATGATTTTGGTATCTGGTTTGGTTAGAAAAAATGTGCACAGTTAGTTTTTRAANNNNTGACTATGAA
TARGET=1541,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05265 Ps_contig_mira-and-tgicl-ass_franssen_37004
SEQUENCE=AATTTTAATATTAAGTAAATAAATATTATTATTGCAATTAACAATACATTTTTGCTCCTTAAAAGCACACACACTTTTTAGGATACTTCACAAGTATCCATTTACATTCAAAAAGAAAACCAATTATTCTAAGCTTCTGTTTTTGTCTTGTAAACAAGACAAACAAGACAATAGTATAACATCATATTGAAAATAAAACTTAGATAATTTAAACCATGAAGCAGCAATATAAATATATATAAAATGTTGCAACACTCACATTAACATTCTCTTTTCAATTTCTCTGTGTTGTCGGTATTTTACTCGATAATACAGAAAATGTTGAAAAGAGAAAATTAGCGTGAGTGAGTGTTGTTGTGTGGAAATAACTCGACCCTATCTATCTATAGAGCCTCGAAGCAGCACCCTTGTCCAAGAACCATTTCACATCTCCTTCAGGATTCACCTTTTGAATCGGAAGCTTATCAGCATTAGGAGCTTTCTCAAGAGCAGAATAAACAGCATCAGCTTTACCGGCACCGGTCACAACCATAGCAATATTTGAAGTAGCATTAATCACTGGCAATGTAAAAGTGATCCTCTCCGGCGGCGGTTTAGGCGCATTCGTAAGATATGTAACCCATTTATTAGTTTCATTCAGTGCAGGGTAGCCTGGAAAAAGAGAGGCTATGTGTCCATCAGGTCCCATATCTAATAGCAAAAGATCAAATTTTGGGAATCCAGTTAGTGATGATGTTGATATCACATTGCTTGTCACTAAACGTCTTAGTGTTGTTTCATAAACATCTGCTGCTCCATCAGCTGGTAAGGAATCATCAATTGTGCTCACATTTAGTGGCGGAATCGGTACCTTGGAGATGAATCCATCATAAGCAAGTTTGTAGTTGCTATCAACATTAGTCTTTGGCACCACTCTCTCATCCACCAAGAAAACATGCCACTTAGCCCAATCAATAGTGTCACAATATGGAGGTTCCACCAGCTTCCTGAGATATTTGACAGGTCCGGGAGACAGAACGACGGTGAAACGTCCCTTCTCTCGAATATATTTCTCGGAGAGGTCAGAAATATATTTGGCCAAAGAAACAGCAAGATGCTCCTTGCTTAGCACATGCACATCCTTGCTATTCATTGACGCCTTCACCAAGCCACCAACATGAATTCTCTTAGGTTTCACTTGAATAGGAGAAGACACATTGTGTCTTAGAGGGTTATAGAAGCATTTTTTACCTAATTGAGGTGACAACAATGACATGGTTGAGGATTGTGTGCGTGACTTATGTTTGGAATGCAACATGCTTTGAGGAGTGCATGTTAGGGACAGAAATGTTGAAGTGGCCATTGATTAGTTAAAAAACAATGAGTTGAATTGAATATTTGTAAGTATTGGAATCNCYTCNCCGTACTNTG
TARGET=376,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05266 Ps_contig_mira-and-tgicl-ass_franssen_37013
SEQUENCE=TCCTCAACTCCGTAATAAAAAGTAAAATRGCTGAAAATCATATCGTGAGGATATCGATACAAATCAAATCAATGTTGCTTCTTCTTCTTCTCAAATCACACTAACACTTTCAACTTAAAACCCAAAAAAAAATAGTTACTCACTCATAACCTTCATCTCCCTTTCACTCATTCACTCTTCACCACCCCCTTCCGCCCCTTGCACCTCACCACTCCAATCCTCAACCTCAAATCACCATCCACACCCCCCAGGTTTACTTCCTTCACCGTCCACGCCGATTCATCTAGCCGTCAATCCCAACCGTCTATCCCCAACCATGTCGTCGCCAATTCCATTTTCGATTCCGTTATCGACTCCTTCCTCTCGCTTCTCGAATTCCTGTGCCTTCTCTCTTCACTCATCGTGTCGGTTAGCGTCGCCGTGATTGCGCTTTGGAAGAAGGAGTTGTATGAAACGATTGGTAACAGAGTTGCTCCGTTGAGTATGTTGTTGTTGGTGGTCGGTGTTTTGACTGGTGCGTTGATTAGGAGAAGGAGGGATATTATGATCACTGGAGTTCCGGTGTCGAAGATGAGTCTGTTGCCGAGGATTCAGAAATTGGAAGGGGATTTGGCTAGGTCTGCCGATGTTATTAGGGTTTTGTCTAGGCAGCTTGAGAAATTGGGGATGAGGTTTCAACTCACTAGGAAGAATTTGAAGGAACCTATATCTGAGACGGCTTCTCTCGCACAGAAGAATTCCAAGGCAGCAACAGTATTAGCCATGCAATCAGACGTTTTGGAGAAGGAGGTCGAGGAAATTCAAAAAGTTTTACTAGCAATGCAGGAACAACAGCAAAAACAACTCGATCTCATTCTTGCAATTGTGAAGCCAGGTAACCCATGGGAAAGCAAGCAAATTAATGAGGTGAATCAGGAGGTCCACCAAATTTGAAGCTTGGTACAAATTCCAGAGTGATGAATCTTAGCGAAACATTCTGATTTTTGATGTGAACACTGAATCATCTATCAACACACGAGGTCAAATTAACAATAATTTTATCACATAAATAATTATTACACTTTTCTAAGGGCACCGTCGGAAGGTTCCTGTGGATACAGGTGAAAAGAAATTAATCAATGAGTCAATTCAATCTAAGGGGAGAATTTACTGAACTCTCTGATTTGTGGTAAAGTTCGGGAACGTGATGTTGGTTCTGTGTTTGTGCATGTACTTTCATTTCAAGTTTACATGGTGCTCATCATCAAAACTTGTCACAGTTCACATCTTTACTTTGTATCAAAAACATATGAATATACATAGGTGTGGAACACTTTGGTCCAGACTAGTGATTGTTCTATTTGGGTCAAGTTTTTCTATATCAAATGAAAAATAGGATTGAAGTCATGGTGGTTGTTTTCACACCCTTGACGGAAAGTGTACCTCAGATTGTTGTTTAGATAATATTAAATTGGATTACACCGTTTTTTTCAAAAATACCTGGACTCGAGTGTTCTTCTGGCTGTTTTCTAAACTATTTCAGTTGTTCTCATCTGAATTTGGTTTGGAAATACTTCAGAGGTTTTTACATCTGGATGTGTATTTCTATCTATTTTTGTGTTACTTTTTTAACTTCTTTCAAATTTTACATTAA
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05267 Ps_contig_mira-and-tgicl-ass_franssen_37014
SEQUENCE=TCAACGCAGAGTACNCGGGGAAGTTAAAATTAAAAAAAAGTAACAAATAATAGTGTGGTGACGCGCTATATGGCGTGTTACCATAGCATAGCAGCGATTTCAGTCTTAGCGGTAAGAGGAGGAGGAGTGGCTATCCATGGTTGGAATTTGAAACACAATCATATCAAATGGTTTTATCTCTAAACCCCATCATCACCTTCTCCTCAACAACGAAGTTGGAAACAAAGTATCATGATGCATCCAGGTACCCAAAGGTCACTCAGATTACGCCCTTTTCGGTAACGTCTCGAACGAGAATGTTCGTCAAGGAGAAAAGAACCGGTCGCTGTTTTTCAGTTGCAAATAGTGATCAGCTTTCAGCAAATGCTAGCAATAAGGATACTGGCAGTGCTGCAAATGATCAGTTACCGTCCATGAATCCTCCGGTTGAGTCAGAATCACAGACACCGAAGGGTTCCAATGGGTCATTAGGATCTGCAGCATCTCCTAATTCACAATCTGTAACCACACGATCGTCTCAGAAATTGAGAGAGAGGATAAAAGCGGCTCGGGTTCTCAACCAGTCAAAGGAACCGAAAGCGTCTAAGTCAGATAAGGGCAAGTCAGATATGGGCAGTAGTGTGTTGGCTGCTTTTAGAGAGGACGATAAAGGGAAGAAGAAGAGAAGATCTGGACTTCCAGAAGCTCCTGGTAATTTGTTTGATGATAGCAAACGAGGGATGCCGAAGGCAGGTTTGACTTTTGATTTTCCCGGAGGTTCTGATCTCTTTTTCATTATCTTTTCATTTGTTTTTATCAGCACAGTGATGTTTGGAACAACTTACATTGTGTGGAAACTTGGTGCAATCCATTTCAATGACTCCTGAGATGAGATTTCAAGAATTCACACTTCACCAGATATATAGAATAGCTTATTTATAATATTTTGACAAGCTTCAGCTTCTATAAACTTCATGACTTGCTGTTGATGTTCTAGTTTTTTTGGTGCTCTAACTTTTTCTTATTTTACTAAAAC
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1379
PRIMER_LEFT_PENALTY=0.026872
PRIMER_RIGHT_PENALTY=0.111077
PRIMER_LEFT_SEQUENCE=CATAGCATAGCAGCGATTTCAG
PRIMER_RIGHT_SEQUENCE=TGTTGAGGAGAAGGTGATGATG
PRIMER_LEFT=81,22
PRIMER_RIGHT=208,22
PRIMER_LEFT_TM=60.027
PRIMER_RIGHT_TM=60.111
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=128
PRIMER_PAIR_PENALTY_1=0.1765
PRIMER_LEFT_1_PENALTY=0.065377
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=CAGAGTACNCGGGGAAGTTAAA
PRIMER_RIGHT_1_SEQUENCE=TGTTGAGGAGAAGGTGATGATG
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=208,22
PRIMER_LEFT_1_TM=59.935
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.50
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=203
PRIMER_PAIR_PENALTY_2=0.2002
PRIMER_LEFT_2_PENALTY=0.026872
PRIMER_RIGHT_2_PENALTY=0.173354
PRIMER_LEFT_2_SEQUENCE=CATAGCATAGCAGCGATTTCAG
PRIMER_RIGHT_2_SEQUENCE=AGGTGATGATGGGGTTTAGAGA
PRIMER_LEFT_2=81,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=60.027
PRIMER_RIGHT_2_TM=59.827
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=0.2090
PRIMER_LEFT_3_PENALTY=0.097964
PRIMER_RIGHT_3_PENALTY=0.111077
PRIMER_LEFT_3_SEQUENCE=CGCTATATGGCGTGTTACCATA
PRIMER_RIGHT_3_SEQUENCE=TGTTGAGGAGAAGGTGATGATG
PRIMER_LEFT_3=63,22
PRIMER_RIGHT_3=208,22
PRIMER_LEFT_3_TM=59.902
PRIMER_RIGHT_3_TM=60.111
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.2138
PRIMER_LEFT_4_PENALTY=0.026872
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=CATAGCATAGCAGCGATTTCAG
PRIMER_RIGHT_4_SEQUENCE=GAAGGTGATGATGGGGTTTAGA
PRIMER_LEFT_4=81,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=60.027
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_05268 Ps_contig_mira-and-tgicl-ass_franssen_37017
SEQUENCE=CGGTACCGATACTCTAAYCAACATGCTTATCAGTATTATATACCAGGAGTTGTTAATCATGTTATGATGCCATCATCAGCACAACTGTATCAAAATAATATCCAGGATCTTCAGAATCATGCTAGTTCATCTATGATTGCCCAGTACAGTCATCTTCCCCAATGTCCACCTCATGCAACTGGGATGGCACCCTACCCGTATTTTCCAATGAATATTTGCTTACAAACTGGTCAGGTTTCTACAACTCATTCATGGCCATCATTAGGAAGTTCAACTTCAAAGTTGAGCAAAGTTGATAGGAGAGAAGCAGCATTGATGAAGTTTAGACAGAAAAGAAAAGAGCGCTGCTTTGATAAGAAAATTAGGTATGTCAATCGGAAACAACTTGCAGAAAGGCGGCCTCGTGTTAGGGGACAGTTTGTCAGAAAGTTGAATGGTATCAATGTGGATCTAAATGGACAACCTGCTTCTACTGACTATGATAATGATGATGATGACGAGGAGGAAGAAAACAATGGCGCGAGAGATTCCTCTCCTAAGGATGCTTGAGAAGATCAATTTCCTTATTTGGCCCAAGAGTGCATCCGCCATGAATTCCTCCCCCGCATAAACGGTTGTCCTGTTCAGAATTTGCCTCTGCTCCTTGATTTGATGGAAAATCTATGCGGTTGGCATTAGTTGACAAACCTGTGCTATTGATTCGAACTTCAGGGATGCTGATTCAGTTCCAGTATGCACCCTCGCCCCGTGGAGTTCAAACAAACTCTTGCTTGGCCTCTTTTGAATATCTCTCTTTCAAATGATTCATCTTGCTGAGCTCTTGCAGAAGATGAAGCTCCTGGTTAAGTCCAATGTACGTTTAAATGTTAATTAAACTTATTAGTTTAATTGGTTAGGTAACTTGGTTTGGAGCGTAGGGTTGTGGTTGCTAC
TARGET=485,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05269 Ps_contig_mira-and-tgicl-ass_franssen_37020
SEQUENCE=AAGCAGTGGTATCAACGCAGAGGAAGGGTGCAGAACAGAATGAAGAGATAAGAGAAAGAGTGAAGAGAAAGATTGAAAAAGTGAAGAAAACTCGTGGCTCGAGCCAATGGTGAGAACTGCCAATTTATAGAGTTTCTATGAAGTGATTTCGTTAACTCTGTTAATCTCTTCTCCCACCAAATTTGTTAACCGTTGAAACTCTGTTAGTTAGTTAGTTTGTTAAATTGATTATTCTGTTAAAATTCTGTTGACAATCTCAAATGGCTTTTGATTCTCTATGATGAATGCAACTTCTGTTATCATGGATGGTTACGNAAAAATGCAGGTGTTTCTGAACTTGGATGCGTAAGCCTCGTCTTTTACTGCGTTTCGCATTTGCTTCAAGCTTGCTTTGGCCGTTTCTGCGTTTGGCATCTTCACATGTACTTGGTGTTGCAAGCTATTTTANGCTGCGCAAAGCCGCGTTCTGCAGTCTT
TARGET=203,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1895
PRIMER_LEFT_PENALTY=0.071684
PRIMER_RIGHT_PENALTY=0.117817
PRIMER_LEFT_SEQUENCE=AAGTGAAGAAAACTCGTGGCTC
PRIMER_RIGHT_SEQUENCE=GCCATTTGAGATTGTCAACAGA
PRIMER_LEFT=78,22
PRIMER_RIGHT=264,22
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=60.118
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.3134
PRIMER_LEFT_1_PENALTY=0.195574
PRIMER_RIGHT_1_PENALTY=0.117817
PRIMER_LEFT_1_SEQUENCE=AAAAGTGAAGAAAACTCGTGGC
PRIMER_RIGHT_1_SEQUENCE=GCCATTTGAGATTGTCAACAGA
PRIMER_LEFT_1=76,22
PRIMER_RIGHT_1=264,22
PRIMER_LEFT_1_TM=59.804
PRIMER_RIGHT_1_TM=60.118
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=0.4385
PRIMER_LEFT_2_PENALTY=0.071684
PRIMER_RIGHT_2_PENALTY=0.366796
PRIMER_LEFT_2_SEQUENCE=AAGTGAAGAAAACTCGTGGCTC
PRIMER_RIGHT_2_SEQUENCE=CATCCAAGTTCAGAAACACCTG
PRIMER_LEFT_2=78,22
PRIMER_RIGHT_2=343,22
PRIMER_LEFT_2_TM=59.928
PRIMER_RIGHT_2_TM=59.633
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.5046
PRIMER_LEFT_3_PENALTY=0.071684
PRIMER_RIGHT_3_PENALTY=0.432914
PRIMER_LEFT_3_SEQUENCE=AAGTGAAGAAAACTCGTGGCTC
PRIMER_RIGHT_3_SEQUENCE=AGTAAAAGACGAGGCTTACGCA
PRIMER_LEFT_3=78,22
PRIMER_RIGHT_3=363,22
PRIMER_LEFT_3_TM=59.928
PRIMER_RIGHT_3_TM=60.433
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=0.5046
PRIMER_LEFT_4_PENALTY=0.071684
PRIMER_RIGHT_4_PENALTY=0.432914
PRIMER_LEFT_4_SEQUENCE=AAGTGAAGAAAACTCGTGGCTC
PRIMER_RIGHT_4_SEQUENCE=CGCAGTAAAAGACGAGGCTTAC
PRIMER_LEFT_4=78,22
PRIMER_RIGHT_4=366,22
PRIMER_LEFT_4_TM=59.928
PRIMER_RIGHT_4_TM=60.433
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_05270 Ps_contig_mira-and-tgicl-ass_franssen_37031
SEQUENCE=TCTTCATGGAGGGYAGNNGGGGAACTACTAGTAAATTACTCTCTCTCTCTCTCTCTCAACCCAAAAGGTCCCCCAACACTCCTTCAAACAACTTTCCATCATCAACAACCACCACCACCTCCTTCATCACCCATCTTCATAATACCCCATCATCTATTTTTTCCAATAAAAGAACTTTCTTACATGGATTTGCAATGAAAGTGAGAGAGTTTAATTATGCGAACGCTTTGTGATGCTTGTGAGAGCGCAGCCGCCATCGTTTTCTGTGCTGCTGATGAGGCTGCACTCTGTCGTGCTTGTGATGAAAAGGTTCATATGTGCAATAAGCTAGCTAGTAGACATGTTAGGGTTGGTCTTGCGAGTCCAAGTGATGTGCCGCGCTGCGACATATGTGAGAATGCACCTGCTTTCTTCTATTGCGAAACAGATGGAAGTTCCCTTTGTTTGCAATGCGATATGATAGTTCATGTCGGTGGTAAAAGAACACATGGAAGATATCTTCTCTTTAGGCAAAGAGTTGAGTTTCCAGGTGATAAACCTAGTAATGCAGAGAATCCGTCTTCGCAACCTTTGGATCCGGGTGATATTAAAAGAGGACAAAGTCCACTTCCTAAACAAAAAATGGGAGAGAAGCAACAGAATCATAGGATGCCTCCGGTTCCAACTTTAGAACCTAATGCCGATGGAAATACCAAGATGGAAAATAAATTGATTGATTTGAACATGAAGCCTAATAGAATACACGATCACGCATCGAATCACCAGCCATAGATTATGCTACTAGACAATGCTATTCAGGCACGAGAAATCAAATCGACGAAAGGCGAAAGCTTCTCGTTATTGTCATGAATGTTAATTACAAAGGTGGCATGTTCTATTGACATCATGTAGTGGCAAATGAGGCATTGGAGCGTCTCAGTCTGAAGAAATTGTTCTCCCTCCCATCATGTAGGAATTTAGTTTTTTTTAGTTAAACAACACATTGTATCTGTACTGCAAAGCATTGTTAATTACAACACCTGTGACCCCTTTCAATGAATTGTGTGTTGAATTTTGTTTTCCAGTTTCAGTTTTGTATGTGACATTTGTGCTCCACCCAGAACATAGAGGATGATACTTTTTCTTTYTTTTTTATAAATAAAAATGTAACATGTGTTTAGYCAAACCACATTTGAAATTTAA
TARGET=39,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05271 Ps_contig_mira-and-tgicl-ass_franssen_37031
SEQUENCE=TCTTCATGGAGGGYAGNNGGGGAACTACTAGTAAATTACTCTCTCTCTCTCTCTCTCAACCCAAAAGGTCCCCCAACACTCCTTCAAACAACTTTCCATCATCAACAACCACCACCACCTCCTTCATCACCCATCTTCATAATACCCCATCATCTATTTTTTCCAATAAAAGAACTTTCTTACATGGATTTGCAATGAAAGTGAGAGAGTTTAATTATGCGAACGCTTTGTGATGCTTGTGAGAGCGCAGCCGCCATCGTTTTCTGTGCTGCTGATGAGGCTGCACTCTGTCGTGCTTGTGATGAAAAGGTTCATATGTGCAATAAGCTAGCTAGTAGACATGTTAGGGTTGGTCTTGCGAGTCCAAGTGATGTGCCGCGCTGCGACATATGTGAGAATGCACCTGCTTTCTTCTATTGCGAAACAGATGGAAGTTCCCTTTGTTTGCAATGCGATATGATAGTTCATGTCGGTGGTAAAAGAACACATGGAAGATATCTTCTCTTTAGGCAAAGAGTTGAGTTTCCAGGTGATAAACCTAGTAATGCAGAGAATCCGTCTTCGCAACCTTTGGATCCGGGTGATATTAAAAGAGGACAAAGTCCACTTCCTAAACAAAAAATGGGAGAGAAGCAACAGAATCATAGGATGCCTCCGGTTCCAACTTTAGAACCTAATGCCGATGGAAATACCAAGATGGAAAATAAATTGATTGATTTGAACATGAAGCCTAATAGAATACACGATCACGCATCGAATCACCAGCCATAGATTATGCTACTAGACAATGCTATTCAGGCACGAGAAATCAAATCGACGAAAGGCGAAAGCTTCTCGTTATTGTCATGAATGTTAATTACAAAGGTGGCATGTTCTATTGACATCATGTAGTGGCAAATGAGGCATTGGAGCGTCTCAGTCTGAAGAAATTGTTCTCCCTCCCATCATGTAGGAATTTAGTTTTTTTTAGTTAAACAACACATTGTATCTGTACTGCAAAGCATTGTTAATTACAACACCTGTGACCCCTTTCAATGAATTGTGTGTTGAATTTTGTTTTCCAGTTTCAGTTTTGTATGTGACATTTGTGCTCCACCCAGAACATAGAGGATGATACTTTTTCTTTYTTTTTTATAAATAAAAATGTAACATGTGTTTAGYCAAACCACATTTGAAATTTAA
TARGET=108,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05272 Ps_contig_mira-and-tgicl-ass_franssen_37038
SEQUENCE=GCGGGGACTTTTTCTTTTTCATTCTCAAGTCAAAAGTGTTATCTTTGTGTTTGAGTTTTCAGAAATGGCAAAATTAGAAGAAGAACCGACATCATCGATTCATCAACAACAACAACCATCTTCATCGTACATATTACTGTTGAATATTATGAGCAAAAGAAGAACATGGGCGTGTCTTTTTTTCCTTGTTTACGGTTACCCTTTTAGCATCTTCATGGAATTTTCTGAAAACAATGCTTTCTTGGTACAACTCACAAGCTCAATCATCAACCTCTGGTTGGCCTGCACTCTACGCATCTGTTCTTCTAGGAACTGTTTTTGGGATGCTTTCAATGGTTGCTGCTTTGGTTGTTATGGTTCCTGCGGTTTTGGTGACGTGGATAACCGTTGTTGTTTTGTTGGCTTTTTTTGGAAAGCCTAGGAGGGTTTTGGTGGTTGAAGGGAGGAAGATTACTGGTGAAATTTTCAGTTTTGTTGTTAAGATTTTGTTGACAGAAGGAAATGTTGTTGCTGCTGTTTGTGCTGTTTTGGGGTATTTTGCTTTGGTTAGAAGGAACAGTGAATGATTTGGGTTTGATTTTGTGTTTTGTTGGTTTTGATTTTTATGAGATTTTGGAAGGTATTGGTAGATAGGGTTTGCAAATCATGCTAGCTGGTTGGTGTGATTTGGTAAAGTGTTTTAATTTATCTGTTTTTTGTTTGTGACATAGCGTAGGAATGAGATTACATAGTTGAAATCAAACATGAAAATGTTGTTTAGATGCTAATCTGGTTTCTGATTATGGTAAATCCATGTGTTGTAGATTCATCAATTTCTTGACTTACTATTACAAGCTCATTAGTTTTACTGGTAT
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6307
PRIMER_LEFT_PENALTY=0.629991
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=AGAAGAAGAACCGACATCATCG
PRIMER_RIGHT_SEQUENCE=AGCATCCCAAAAACAGTTCCTA
PRIMER_LEFT=75,22
PRIMER_RIGHT=327,22
PRIMER_LEFT_TM=60.630
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.6000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.6434
PRIMER_LEFT_1_PENALTY=0.629991
PRIMER_RIGHT_1_PENALTY=0.013401
PRIMER_LEFT_1_SEQUENCE=AGAAGAAGAACCGACATCATCG
PRIMER_RIGHT_1_SEQUENCE=GAAAGCATCCCAAAAACAGTTC
PRIMER_LEFT_1=75,22
PRIMER_RIGHT_1=330,22
PRIMER_LEFT_1_TM=60.630
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.6641
PRIMER_LEFT_2_PENALTY=0.629991
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=AGAAGAAGAACCGACATCATCG
PRIMER_RIGHT_2_SEQUENCE=TCCATGAAGATGCTAAAAGGGT
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=218,22
PRIMER_LEFT_2_TM=60.630
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=0.7110
PRIMER_LEFT_3_PENALTY=0.710267
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=GGCAAAATTAGAAGAAGAACCG
PRIMER_RIGHT_3_SEQUENCE=AGCATCCCAAAAACAGTTCCTA
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=327,22
PRIMER_LEFT_3_TM=59.290
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.7444
PRIMER_LEFT_4_PENALTY=0.710267
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=GGCAAAATTAGAAGAAGAACCG
PRIMER_RIGHT_4_SEQUENCE=TCCATGAAGATGCTAAAAGGGT
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=218,22
PRIMER_LEFT_4_TM=59.290
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_05273 Ps_contig_mira-and-tgicl-ass_franssen_37042
SEQUENCE=GGGTTAAACGGTTTTACGGGGTCCCCGGGGACCCCCCCCGGGACCCGTACGGTAGGGGGGCGGATACGGCCGAACTGGCCCGTCGCCCCCCCANCCNATCAGCCCGACGCTTTATAGTGTAAGATGACGGTCTGCTGCAGCTACAGATATCTTTCAGCTGATTGGAGTGCAGGCATGCAGGCCGAGTTTGGCTGGAATGGATTTGTGTATTTCTTAAAAAACCAACTCAAACAAGGATCCTTTCCTCTAATCTCGGTTTACGAAGGATGCTTCTTGCATCTTCTATGAGTTACATGACTATGTTTCTCTTGTGGACATTTGGTATTTTGTGAGGAGCTTTTGGAGCGCGCACACACACATACTGCTGCTCATGAAGATGAGTGAACTGAGGACTTCTGTAGAGCCATACAATTCACCTAATTCATTTCTTGAGAATTGGGTATGCGAATATAGTGGAATCATCAGAGGGTCACTTCAATGTTTCATTGTTTGAGCTCCCCAAGAGAGCTAAAGGGATTCAAGTCTTGCTTGGTGTCATTTGCCTTCAGATTTGCAGGAGCTTTTGTGTTGAAGGTTTAACTGGAGTTTTATAGCTCTAGTTACTATTCCCTATATACTTCATAGTCCAGCAAATAATCCGTAACCTCATGTATGGCTAACTTCTGTGGATGAGTTTGTATATTTTACTCTCTTCATACATTTCTTAGTTCGATATTTGCTACAAGTTTTAACAATTTTTTATACAGTGATCTTTTCANTA
TARGET=350,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0667
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.058463
PRIMER_LEFT_SEQUENCE=TGTTTCTCTTGTGGACATTTGG
PRIMER_RIGHT_SEQUENCE=GAAGTGACCCTCTGATGATTCC
PRIMER_LEFT=300,22
PRIMER_RIGHT=475,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.942
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.1193
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=TGTTTCTCTTGTGGACATTTGG
PRIMER_RIGHT_1_SEQUENCE=CATTGAAGTGACCCTCTGATGA
PRIMER_LEFT_1=300,22
PRIMER_RIGHT_1=479,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.1403
PRIMER_LEFT_2_PENALTY=0.081801
PRIMER_RIGHT_2_PENALTY=0.058463
PRIMER_LEFT_2_SEQUENCE=GGCTGGAATGGATTTGTGTATT
PRIMER_RIGHT_2_SEQUENCE=GAAGTGACCCTCTGATGATTCC
PRIMER_LEFT_2=189,22
PRIMER_RIGHT_2=475,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=59.942
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.1578
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.149516
PRIMER_LEFT_3_SEQUENCE=TGTTTCTCTTGTGGACATTTGG
PRIMER_RIGHT_3_SEQUENCE=TGAATCCCTTTAGCTCTCTTGG
PRIMER_LEFT_3=300,22
PRIMER_RIGHT_3=519,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.850
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=220
PRIMER_PAIR_PENALTY_4=0.1929
PRIMER_LEFT_4_PENALTY=0.081801
PRIMER_RIGHT_4_PENALTY=0.111077
PRIMER_LEFT_4_SEQUENCE=GGCTGGAATGGATTTGTGTATT
PRIMER_RIGHT_4_SEQUENCE=CATTGAAGTGACCCTCTGATGA
PRIMER_LEFT_4=189,22
PRIMER_RIGHT_4=479,22
PRIMER_LEFT_4_TM=60.082
PRIMER_RIGHT_4_TM=60.111
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_05274 Ps_contig_mira-and-tgicl-ass_franssen_37044
SEQUENCE=ACACGATAATAGGAACTAGAGAGAGAGAGTAGTAGCCATGGTTACGAAACTGGATATAGATGATTTGCCAAAGAACGCGGCGAACTACACAGCTTTAACGTCCGTTATGGTTCTTACAGCGAGCAGCTCAAGTTCACCCGACCAGAAAATCAGTTATCCACGGATCCCGTCAGTACACGTGGCACCAGACTTACCAACGTTGCCGTCGATTCGCCTCCGCTCTTTCCAAACTCTCCATCGGTCCCGGAAACACTGTAGCTGTTATTGCTCCCAATATTCCAGCTCTCTATGAAGCTCATTTTGGGATTCCGATGGCAGGGGCGGTGTTGAACCCGGTGAATATAAGGCTAAATGCAGCCACTATTGCTTTTCTTCTTGGTCATTGCACTGCGTCGGTTATAATTGTCGATCAAGAGTTCTTTTCGTTGGCGGAACAAGCTTTGAAAATATGGTCTGAGAAATCGGAGACATTCAAGTCTCCGATTTTGATTGTTATTGGAGATGAAAACTGCGACCCTGAGTCACTTAGATATGCGTTGAGCAAAGGAGTTGTTGAGTATGAAGATTTTCTTCAAAGTGGTGACCCTGAATACGATTGGAAGCCGCCGGAGGATGAGTGGCAGAGCATTTCCTTGGGTTACACGTCTGGGACGACGGCTAGTCCTAAAGGTGTGGTTTTGCATCACCGTGGAGCGTATCTCATGGCTCTTAGTTGTGCACTTATTTGGGGAATGAATGAAGGAGCTGTATATCTTTGGACTCTACCCATGTTTCATTGCAATGGCTGGTGCTACCCTTGGACGCTTCCTGCTATCTTTGGCACAAACATATGCCTCCGCCAGGTTTCTGCAAAGGCAGTATACGAAGCCATTGCCAAGTACAAAGTGACTCATTTTTGTGCAGCACCAGTGGTGCTTAACTCAATCATTAATGCTCCTGCTGAGGATACCATACTTCCTCTACCTCACGTTGTACATGTGAACACTGCCGGTGCTGCTCCTCCTCCTTCTGTTCTTTCCGGCATGCATGAAATAGGATTTCGTGTCACACACACTTATGGTCTTTCAGAAACCTTTGGCCCGTCTGTCTATTGTGCCTGGAAACCAGAGTGGGACTCACTTCCTCCGGAAGCCCAAGCCCGGCTCCATTCAAGACAGGGGGTTCGGTACATTGCCTTGGAAGGCCTGGATGTCGTCAACACAAAAAACATGCAACCTGTTCCTGCAGATGGCAAAACTGTTGGTGAGATCGTGATGCGTGGTAATGCTGTGATGAAAGGCTACTTAAAGAATCCTAAAGCGAATGAAGAAAGCTTTGCAAATGGATGGTATCATTCCGGCGATCTTGCTGTAAAGCACCCGGATGGATATATAGAAATTAAAGACAGATCAAAGGACATCATCATCTCGGGCGCTGAAAATATCAGCAGTGTAGAAGTAGAGAATGCTCTTTACTCGCATCCTGCAATCCTGGAGACATCAGTGGTTGCAAGGCCAGATGAGAAATGGGGCGAGTCTCCATGCGCGTTTGTTACATTGAAGCCTGGAGTGGATAGTAGTAACGAGCAGCGTCTAGTCGAAGATATACTGAAGTTCTGCAGGTCAAAGATACCTGCTTATTGGGTCCCAAAGTCGGTTGTGTTCGGACCGTTACCTAAGACAGCCACCGGGAAGGTTCAGAAGCATCTGCTGAGGGCCAAGGCAAAGGAGATGGGGCCTATCAAGAATAAGATTAGCAAGCTGTAATCAATTAAGAGTGACAATGTCGATAGAAGATAAGAGTTCTTGCTATTGTATGTGGCATGTGTTATTAACATGGTGATAATCAGTACGAATTATTGTTCTATAATAATTAATAATAAAAGCTTTTTAGCTGTTCCTAAACCTAGTTCATTGTTTTTATTCTATTATGCAATTTGGGTCCT
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05275 Ps_contig_mira-and-tgicl-ass_franssen_37048
SEQUENCE=GTCRGGGGCACCCAATTAAAACAAATCAAAATTCAATCATCCAGATTTCTCCGACTTATTACTCTTCCTTAACCTATATCTTCAACTTCGCTCAAATTAGTTACTAAATCCTATTTTATTCCTCGATTTCGATTCTCCGCTTAGATTTGGGATTAGGGTTTTTCATTTCCAGAAACCGTTCATTTCTCGTACAAATGGTTGAACAATCGCTTCTATCAATAACAAACCAAGTTTCAGATTCTTCCACTAGAGTATTATCAAAGGGATTCACCGATTGGATTTTCAATTGTCACGGGTTCTGGCACAATGTTGTGTTGCTTGTTGCCTCTTTTCTCTTCGTTTTGTATCTGGCACTTCAAGCGAGGAATAGCTTCCTTAAACTCACCAATGGCCGCTCTTACATCATTATCTCTTATTACGTCTCTCTTTGGCTTGTCAGTCTTCTAAATCTCGCTTGGTGCTTTTCTCAGGCATGGGAGTGTTCTGCTGGGAAAGAGCTCACTTGGAACTTGTTGTCCTTATTTACGTCTTCTGGAATGCTGTTTCTAGAAGTGAGCTTGCTGGCGTTTTTACTTCAGGGCAATAATGCAAGTGGCGTAGAGGCGTTGACACGAACTTTTGGTATATCTGGTATCATTGTCGGTTTTGATGTCTTGTTAAAGGCTATCTATCTGTTTGCATTTGGGGTCCCATTGTTCATCGACACTGATTACAGTACTCCCCGTGTGAAGTGGAACTTATGGGTTATTCACAAGCTACTCCTCACTGGTGTTTATGGGCTCATATTGTTTATGTATCATTCTAGGTGGAGAGAAAGGTTACCCGCAAGACCGGCGTTCTACAATTATGTTATTATTATGTTCATCTTGAATGCAATTGCGTTGTTTGCTTGCGGGCTTACTGGAAATGGCGCTGGTTTTGCTTTTTGGTTTTATCGCATTACCGTTGTGTGCTACCATGCCTTTTATCTTCCACTGCTGTATATTACATTTCTGGCAGACTTTTTCCAGGAGGAAGATTTTCACATGGAGAATGTGTATTACTCTGAAATGAAAGATGCTGGTTTCTTCGAATCCGATTGGGAGTGATGTGGTATCAAGTTTTAACTGTAAGATTAGATTAATAAGTCCTCAATATATCCATTGCAGGACATAACTTTCACGAAATTTGAAATTTGTAAATAAGTAAATATGTTTGTAGATCGCATTGTATATCTTTCTTTCTTTTTGGAAAATTATTTTTAGGACAGTTACCTCGAGTTATTTTGACGGACAATATCCTATCCTATGTGTGTT
TARGET=1214,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05276 Ps_contig_mira-and-tgicl-ass_franssen_37049
SEQUENCE=AAGAAATAAGATAATCGTTGTTTTTAACTAAATCAGACCATTACTAAATGCCGTCTAGCATCCATTCTTAGATCTTCTTTCATCTTTCTGATAAAATTTTCGAACTTTTGGTTTAACTCTTCTGTACTTAATATACAACTTTCTTCTTCTTCTTCTACATAATCTTCTTCTTCCTCTTCTTCTATATTGTTTTCTTCAATTACAACGAAATCAAATTCTGATCCTTTATCTTCCACTTCGTCGTCTCCATCAAATAGTTCAACTTCTTCCTCTTCTACCTCCTT
TARGET=141,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0817
PRIMER_LEFT_PENALTY=0.478260
PRIMER_RIGHT_PENALTY=0.603423
PRIMER_LEFT_SEQUENCE=CTAAATGCCGTCTAGCATCCAT
PRIMER_RIGHT_SEQUENCE=CGACGAAGTGGAAGATAAAGGA
PRIMER_LEFT=43,22
PRIMER_RIGHT=242,22
PRIMER_LEFT_TM=60.478
PRIMER_RIGHT_TM=60.603
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=200
PRIMER_PAIR_PENALTY_1=1.6939
PRIMER_LEFT_1_PENALTY=1.346747
PRIMER_RIGHT_1_PENALTY=0.347129
PRIMER_LEFT_1_SEQUENCE=TCAGACCATTACTAAATGCCGT
PRIMER_RIGHT_1_SEQUENCE=ACGACGAAGTGGAAGATAAAGG
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=243,22
PRIMER_LEFT_1_TM=58.653
PRIMER_RIGHT_1_TM=59.653
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=1.6939
PRIMER_LEFT_2_PENALTY=1.346747
PRIMER_RIGHT_2_PENALTY=0.347129
PRIMER_LEFT_2_SEQUENCE=CAGACCATTACTAAATGCCGTC
PRIMER_RIGHT_2_SEQUENCE=ACGACGAAGTGGAAGATAAAGG
PRIMER_LEFT_2=33,22
PRIMER_RIGHT_2=243,22
PRIMER_LEFT_2_TM=58.653
PRIMER_RIGHT_2_TM=59.653
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=1.7242
PRIMER_LEFT_3_PENALTY=1.377092
PRIMER_RIGHT_3_PENALTY=0.347129
PRIMER_LEFT_3_SEQUENCE=TCAGACCATTACTAAATGCCGTC
PRIMER_RIGHT_3_SEQUENCE=ACGACGAAGTGGAAGATAAAGG
PRIMER_LEFT_3=32,23
PRIMER_RIGHT_3=243,22
PRIMER_LEFT_3_TM=60.377
PRIMER_RIGHT_3_TM=59.653
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=1.7580
PRIMER_LEFT_4_PENALTY=0.478260
PRIMER_RIGHT_4_PENALTY=1.279754
PRIMER_LEFT_4_SEQUENCE=CTAAATGCCGTCTAGCATCCAT
PRIMER_RIGHT_4_SEQUENCE=ACTATTTGATGGAGACGACGAA
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=60.478
PRIMER_RIGHT_4_TM=58.720
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_05277 Ps_contig_mira-and-tgicl-ass_franssen_37055
SEQUENCE=ATTGNGGGGAAATATTAATTCAACGATATCAGAGAGAGAGAGTAAGAGAGAGAAAGATGGCTGAGTTGCAGAAATCATTAGCATCATTCAGGAGACAAGGCTCATCAGGATTGGTTTGGGATGACAAGTTCCTGTCTGGTCTGAATCAGAACCAACAACAACAACAAGAGGAAGAGAGTAACACCGTTGGATCAAGTGCCACGTTGGATAGAAGTAGATCAGCTGGAGCACGACCATATCGGACGGTCAACGTTTCACCACCATCGAAGGACCCACCTTCTCCTAAGGTAGCAACCTGTGGTTTTTGTGGTTTCTTTCGGGAATCAAATAAACCAAAATCTGGAAAAAAAAGATGATGGTAATTCTTCAACCTGTATGTAAATTAGTGCTTGATCATGTTGTTTATCCTATATAGTAGTAATTGTTTGTTTCGGTTTCAATTTTGTGTTAATTATTTGGGATTGTTATTTGTAAATTTGTAGAACATATGAAAATATAGGTGTTGTGTAATAAGCTTTGCGCAAATTTGGTGTGAAACCGATGGTTTTTTCAAAACACTTTTTAGTTTTTCGAAACT
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05278 Ps_contig_mira-and-tgicl-ass_franssen_37055
SEQUENCE=ATTGNGGGGAAATATTAATTCAACGATATCAGAGAGAGAGAGTAAGAGAGAGAAAGATGGCTGAGTTGCAGAAATCATTAGCATCATTCAGGAGACAAGGCTCATCAGGATTGGTTTGGGATGACAAGTTCCTGTCTGGTCTGAATCAGAACCAACAACAACAACAAGAGGAAGAGAGTAACACCGTTGGATCAAGTGCCACGTTGGATAGAAGTAGATCAGCTGGAGCACGACCATATCGGACGGTCAACGTTTCACCACCATCGAAGGACCCACCTTCTCCTAAGGTAGCAACCTGTGGTTTTTGTGGTTTCTTTCGGGAATCAAATAAACCAAAATCTGGAAAAAAAAGATGATGGTAATTCTTCAACCTGTATGTAAATTAGTGCTTGATCATGTTGTTTATCCTATATAGTAGTAATTGTTTGTTTCGGTTTCAATTTTGTGTTAATTATTTGGGATTGTTATTTGTAAATTTGTAGAACATATGAAAATATAGGTGTTGTGTAATAAGCTTTGCGCAAATTTGGTGTGAAACCGATGGTTTTTTCAAAACACTTTTTAGTTTTTCGAAACT
TARGET=153,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1177
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.022096
PRIMER_LEFT_SEQUENCE=ATTGGTTTGGGATGACAAGTTC
PRIMER_RIGHT_SEQUENCE=GCTACCTTAGGAGAAGGTGGGT
PRIMER_LEFT=109,22
PRIMER_RIGHT=291,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=60.022
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.1177
PRIMER_LEFT_1_PENALTY=0.095574
PRIMER_RIGHT_1_PENALTY=0.022096
PRIMER_LEFT_1_SEQUENCE=GATTGGTTTGGGATGACAAGTT
PRIMER_RIGHT_1_SEQUENCE=GCTACCTTAGGAGAAGGTGGGT
PRIMER_LEFT_1=108,22
PRIMER_RIGHT_1=291,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.022
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.2851
PRIMER_LEFT_2_PENALTY=0.095574
PRIMER_RIGHT_2_PENALTY=0.189529
PRIMER_LEFT_2_SEQUENCE=ATTGGTTTGGGATGACAAGTTC
PRIMER_RIGHT_2_SEQUENCE=AGAAACCACAAAAACCACAGGT
PRIMER_LEFT_2=109,22
PRIMER_RIGHT_2=314,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=59.810
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.2851
PRIMER_LEFT_3_PENALTY=0.095574
PRIMER_RIGHT_3_PENALTY=0.189529
PRIMER_LEFT_3_SEQUENCE=GATTGGTTTGGGATGACAAGTT
PRIMER_RIGHT_3_SEQUENCE=AGAAACCACAAAAACCACAGGT
PRIMER_LEFT_3=108,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.810
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=0.2917
PRIMER_LEFT_4_PENALTY=0.269585
PRIMER_RIGHT_4_PENALTY=0.022096
PRIMER_LEFT_4_SEQUENCE=AAGATGGCTGAGTTGCAGAAAT
PRIMER_RIGHT_4_SEQUENCE=GCTACCTTAGGAGAAGGTGGGT
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.270
PRIMER_RIGHT_4_TM=60.022
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_05279 Ps_contig_mira-and-tgicl-ass_franssen_37058
SEQUENCE=TTAARTAATCCACCAATGACATCTTTATCGGAATAAAATCGGTGAAAATCATCGTCACAAACAACACAAACTTCTCTATCATTTTAACTTGATATTGTCTAATAATCAGACTACCTTTGAGAACAAAGTAACCCATTCCCAAACTCATCTACAACACATATATGTACATATCGCGTTGTAGCATAATTTAAAAATATAAATGAACAGAATAAAAGGTAAATAAAAACCTTTGCTATTATGGTCAACCACAAGAGCTTTAACATTATGTAACCGTTATATTATGCCATGGATCTTTGAATATCATCATCACCTCAGAATCGATACAACTGACCATGCAAATCTACGTCTTTCCGAGCTTAATTTAACCTGACGAATACATCTTAGTTAATTAAGGAATAGTGATGTGGCAACCATCACATCTTGTTTGACATCACGAACTTTGTTTTTTTCTTCTGCAGGTTTAAGAATCTGGAAGGAGCACATCAAGTTTTCGTCAACACTCATCATTGCACCAGCATTGTCAAATTCACCACAGTAGTTTGGAGCTGAAAAAATCGTAACAAGTTGCCTGTCTGCAAAGAATTCATATCCATCCTCTACGACCTGATGAGCACGGCAAATAAGGTCCAAGTCATGCATTGTCAAAAATTCAGCCACTTTATCAGGACCAAAGGTGTAGGACACTCCTCTATCGTTCATGCCCCAACCCTTCACATCTCTACCAGGATCAGACCAAAGCAAATCACAGAGCAAACCAGTGTCAGGGATAGCAACTGGACGAGGCAGAATTCTGATTTGATCCAAATTAGTGAGATCGGGGGAAAGACCGCCATGCATGCACAATATCTTATCATCTATGAGAGCTGCAACGGGAAGGCAGTTAAAAGAGTCGGTAAAGGCTTTCCAAAGCCTCACGTTAAACCTTCGCTTACATTCATCGTAAAATCCATATATCCTATTAATGGAAGCGCACTCGTGATTTCCCCTCAGAAGGAAAAAGTTTTCAGGATATTTGATTTTATAAGCAAGCAAAAGACATATGGTTTCTAAGCTCTGCTTGCCACGATCCACGTAGTCCCCTAAAAAGAGATAATTAGATTTTGGAGGTAAACCCCCATACTCAAAGAGCCTTAACAAATCACTGTACTGCCCATGAATGTCACCACAAATCTTGATAGGGGCTTCAAGTTCAAGCAAATTAGGTTGTTGAAGGAAGATATCTCTAGAAGCAAGACAAAGTTGCTTGATCTCAGCCTCAGATAGCTGAACTTGCTTTCCGGGACGCGACAATCTGACCTCGGTGAGTCGGCGGATAATGTCATCCAGCACCGCTTCATCAATCCCTTGAACTTGTGCTTGAACTTGTGCTTGTCCTGGTGCACTCATTGATTCCGATCAACAATAACAACTTCTTCAGTCTCTGAATGAAGAACGGAAGAAGAAGAAGGAAGCAAAAGGGTGAACCAACCAAGAGAGAGAGTAGAGAGAGTGGCTCAATCTCTTTTACTTTGGTTGGTTCTCTCTCATTGTCYTCGCGTTACTGT
TARGET=1435,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05280 Ps_contig_mira-and-tgicl-ass_franssen_37058
SEQUENCE=TTAARTAATCCACCAATGACATCTTTATCGGAATAAAATCGGTGAAAATCATCGTCACAAACAACACAAACTTCTCTATCATTTTAACTTGATATTGTCTAATAATCAGACTACCTTTGAGAACAAAGTAACCCATTCCCAAACTCATCTACAACACATATATGTACATATCGCGTTGTAGCATAATTTAAAAATATAAATGAACAGAATAAAAGGTAAATAAAAACCTTTGCTATTATGGTCAACCACAAGAGCTTTAACATTATGTAACCGTTATATTATGCCATGGATCTTTGAATATCATCATCACCTCAGAATCGATACAACTGACCATGCAAATCTACGTCTTTCCGAGCTTAATTTAACCTGACGAATACATCTTAGTTAATTAAGGAATAGTGATGTGGCAACCATCACATCTTGTTTGACATCACGAACTTTGTTTTTTTCTTCTGCAGGTTTAAGAATCTGGAAGGAGCACATCAAGTTTTCGTCAACACTCATCATTGCACCAGCATTGTCAAATTCACCACAGTAGTTTGGAGCTGAAAAAATCGTAACAAGTTGCCTGTCTGCAAAGAATTCATATCCATCCTCTACGACCTGATGAGCACGGCAAATAAGGTCCAAGTCATGCATTGTCAAAAATTCAGCCACTTTATCAGGACCAAAGGTGTAGGACACTCCTCTATCGTTCATGCCCCAACCCTTCACATCTCTACCAGGATCAGACCAAAGCAAATCACAGAGCAAACCAGTGTCAGGGATAGCAACTGGACGAGGCAGAATTCTGATTTGATCCAAATTAGTGAGATCGGGGGAAAGACCGCCATGCATGCACAATATCTTATCATCTATGAGAGCTGCAACGGGAAGGCAGTTAAAAGAGTCGGTAAAGGCTTTCCAAAGCCTCACGTTAAACCTTCGCTTACATTCATCGTAAAATCCATATATCCTATTAATGGAAGCGCACTCGTGATTTCCCCTCAGAAGGAAAAAGTTTTCAGGATATTTGATTTTATAAGCAAGCAAAAGACATATGGTTTCTAAGCTCTGCTTGCCACGATCCACGTAGTCCCCTAAAAAGAGATAATTAGATTTTGGAGGTAAACCCCCATACTCAAAGAGCCTTAACAAATCACTGTACTGCCCATGAATGTCACCACAAATCTTGATAGGGGCTTCAAGTTCAAGCAAATTAGGTTGTTGAAGGAAGATATCTCTAGAAGCAAGACAAAGTTGCTTGATCTCAGCCTCAGATAGCTGAACTTGCTTTCCGGGACGCGACAATCTGACCTCGGTGAGTCGGCGGATAATGTCATCCAGCACCGCTTCATCAATCCCTTGAACTTGTGCTTGAACTTGTGCTTGTCCTGGTGCACTCATTGATTCCGATCAACAATAACAACTTCTTCAGTCTCTGAATGAAGAACGGAAGAAGAAGAAGGAAGCAAAAGGGTGAACCAACCAAGAGAGAGAGTAGAGAGAGTGGCTCAATCTCTTTTACTTTGGTTGGTTCTCTCTCATTGTCYTCGCGTTACTGT
TARGET=1471,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05281 Ps_contig_mira-and-tgicl-ass_franssen_37065
SEQUENCE=AATTTGCAGCAAATGCAGTTGTTCCCTTCTAAATGCATGCAGAGAAGAACCTGAAATACAACAATCGGCACTTTCATGAAATGACCACCTATGTCTTGCAGGCTACATATTCTCGCCTGCCGTTGATCTCCTTCTGAAGAAACCATTAAACCACTGTTCAAGTCAAGATATCTAACTGTAGTAGTAGTAGTAGAAGAACTCGGTTCTCTGGAACTTCTATGAATGACAGGATTCGACCACTTTGTACATACAAGAAACGCAAAGCACTCGGCAATACTGAAGTTAATAAATAAGTCCCACCAACCGAGAGAAGCTACACTACCGTTCAGTTTTAGGAGTGTGAAAACGGTAGCAGTAATGAAGAAAACCATAGAGATGGCAACCCAGAAGTGAGGAAGAGTTTCCCATATTGCTTCATCACTCATGCTTTCTTCATCCCCTGGCATTAGAGCTCTACACATTCTGAAATTGTCAATGAGAATAATGATTTCAAATGTTAATAAAGGAAGGAACACAATCTTCAGATCTACTGCTGCAAAGCCACGAACATATAAGCTCTCAAGATATATACAAAGGAGCAATTCAAACGCAATAAGCAATGGCGTTGCGACAACGGCATGACACGGCGCCCAATGTCGATTACGCGGAGCTGAGGGCGCAGGTAATGAAAACCTTCCTCGAGCCACAACGCCATGGAACATCCAAAGAGGAGAAAAAATCATCCACCAAGAGGAAGAGATGTGATGATCAAGCTTGAGAAGGAGCAAGAGAGTGAAAGAGACGAGAAAAGTGTGGGCGGCGAGTGCCTGCGCTGATTTCAACACCCTACTCCAGCTCATGTTGTGAAGAGAAATGGTTCTGGTTTCTTTCAAGTTACAACCTTGGAATAGAGAGTGAGTCAATTCAAATTCACGTCAAACAAACACGAACCCAATTCTACCCT
TARGET=178,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1663
PRIMER_LEFT_PENALTY=0.135999
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=GCAGTTGTTCCCTTCTAAATGC
PRIMER_RIGHT_SEQUENCE=TGTACAAAGTGGTCGAATCCTG
PRIMER_LEFT=14,22
PRIMER_RIGHT=247,22
PRIMER_LEFT_TM=60.136
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.2658
PRIMER_LEFT_1_PENALTY=0.135999
PRIMER_RIGHT_1_PENALTY=0.129818
PRIMER_LEFT_1_SEQUENCE=GCAGTTGTTCCCTTCTAAATGC
PRIMER_RIGHT_1_SEQUENCE=AAAGTGGTCGAATCCTGTCATT
PRIMER_LEFT_1=14,22
PRIMER_RIGHT_1=242,22
PRIMER_LEFT_1_TM=60.136
PRIMER_RIGHT_1_TM=59.870
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=229
PRIMER_PAIR_PENALTY_2=0.2742
PRIMER_LEFT_2_PENALTY=0.243965
PRIMER_RIGHT_2_PENALTY=0.030276
PRIMER_LEFT_2_SEQUENCE=AACCTGAAATACAACAATCGGC
PRIMER_RIGHT_2_SEQUENCE=TGTACAAAGTGGTCGAATCCTG
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=247,22
PRIMER_LEFT_2_TM=60.244
PRIMER_RIGHT_2_TM=60.030
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.2797
PRIMER_LEFT_3_PENALTY=0.243965
PRIMER_RIGHT_3_PENALTY=0.035772
PRIMER_LEFT_3_SEQUENCE=AACCTGAAATACAACAATCGGC
PRIMER_RIGHT_3_SEQUENCE=CGGTAGTGTAGCTTCTCTCGGT
PRIMER_LEFT_3=47,22
PRIMER_RIGHT_3=323,22
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=59.964
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.2999
PRIMER_LEFT_4_PENALTY=0.269585
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=AAATGCATGCAGAGAAGAACCT
PRIMER_RIGHT_4_SEQUENCE=TGTACAAAGTGGTCGAATCCTG
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=247,22
PRIMER_LEFT_4_TM=60.270
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_05282 Ps_contig_mira-and-tgicl-ass_franssen_37067
SEQUENCE=GTCGCTTGATAGTACTACTCTTTATCATCAATTGTCTAAAGTAGATAATAATAATACAACAACAACCATACACTTTGCATGAACATAAAGTTTCTCACACACCAAAGTAYCAAATCAATCCCACATACATGAATATTAATTAATCACCCCCTCTTCTATACCATTCATTATACCCCAAACCCCAATAATCTCCATAACCTAACCTCTCCCAAATGTTAAGAAAGTTAACAAACATTAGTTTCTTTCATGAGAAAAGAAACTAATGACAATACATTAGAAAAAAGAGTAAAGAAAATAAAGGATATTCATATGTCATTGCATATACACATTACAAAACTAATGAAAATGACCAACTTCAACTTACTCAGTACTCAGTAGTAGTACTCACCAAATGAAGAACCTTCATTTCTTGATCTTCATCCATGGAGTTACACACATGATGAGAGAAGAGGATCCGAGGTTCCTCTCAGCACAAATTTCTCAACCTTGAGACATAAAACTAACAAGGTTGAAGACTAGTATAGTAATATATTTGCAGCTGACATGATCTCTCTGATCCTAAACTCTCTCACCTTTTCTCTATTTTTTTTTTCAATGTAAGTAAACCAAACCCAATTGACCCCAAAAGAAGAAAAAAACAGCATTTTTCAGCAATAAACAGAAGCCAAAAAGAGATAAGAATAAGATAGTTTTGATCTTTCTAGATCAGAGAAGAAACTGAATTAGAACCAGGTTGATCAGTACTCCAAACACTACYCAAACCTGTTGCTGTTGCTGNTGTGTTGTTGTTGCTGTTGTTGTGATTATTATTCCAATAAAGAGAAGCATTATTATCAGACAAACCCATATTCTCCATCTGGTTTTGAAAAACAGCACCACCATTACCATTCAGATTATTCATATTCCACTGATCCAACCTATTTTGTAGCCCTTCACCTAATTTCACTTCTTTTGAATCCATCTCTTGATGATGATGAAAAGGATCATCATCACCACCACCACCACCACCACCACCTTTTATTCCACCACCAGTACTCATGAACTTCTGTTGCAGTAGTGTAGAGCTAAGTAGAGAAGCCATAACAGGAGTAGTTGAAGTAGAAGAAGAACCAAAGATGGAACTGTAATTGTTATTATTTGAAGTACTAAACCCATTTCTGTAACTACCCTCATCATGATGATGATGATGATGATGATTAAGATTAACTTCTCCTCCAGCAGCAGACATTAGTATCCCAGATGAAAAACCTAATCCAAGACCATTCTGGGGAAGATTATCATAACCAGAAGCAGGATTTGAAACTCTAGTAGTAGAATTAAACCTTGTTGAAAAAGGAAAATTCATAACATCATGACAAGAGTTACCACCATAAAGCATAGTATTGATATGGTTTGAGGTAGCAGCAATATCAAAACTAATGGTGCTGTTGTTGTTGTTGTTGTTGTTGAGATTGAGAAGGAGGGTTTGGATTAGCAACAACACTAGAAGGAGTTGAAGAAGAAGGAGAAGCAGAAGAATCAATGTTGTTGTTGTTGGAACAAGAGTATGTTGGTCGTTTAAGACGTTTGTTTTTTCTGCAACCACCACCAACAGGAACATTTCTGAGTGTTCCACCTCTTGTCCAGTATCTTTTACAGGCTTTGCAGAAGTGTCTCGGTTGCGTTAAACTGTAGTTATTGTAGTAACAGAATTTGGTGTTGGATGAATCACAACGTGGGCATCTTAAAGCTTGTTGTTGTTGTTGAAGAAGCTCTTGACCTGGTTTTTCCATAACCCTATTACCGGTTGAAGAAGCCATTGAAACCTTATGATCATCTATCTCACTCTACAATCAAAAGGATAAGAAATAAAAATGAATAAACTAATTGAAAAACAGAAGAGANACCRTTACCAAACATTTTGGTTTCTATGAGAGTAGTTGGTGCCTTGAGTCTTGTTTGATGGTCTTAGAAATGGAGTGAAGGTGGC
TARGET=45,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05283 Ps_contig_mira-and-tgicl-ass_franssen_37067
SEQUENCE=GTCGCTTGATAGTACTACTCTTTATCATCAATTGTCTAAAGTAGATAATAATAATACAACAACAACCATACACTTTGCATGAACATAAAGTTTCTCACACACCAAAGTAYCAAATCAATCCCACATACATGAATATTAATTAATCACCCCCTCTTCTATACCATTCATTATACCCCAAACCCCAATAATCTCCATAACCTAACCTCTCCCAAATGTTAAGAAAGTTAACAAACATTAGTTTCTTTCATGAGAAAAGAAACTAATGACAATACATTAGAAAAAAGAGTAAAGAAAATAAAGGATATTCATATGTCATTGCATATACACATTACAAAACTAATGAAAATGACCAACTTCAACTTACTCAGTACTCAGTAGTAGTACTCACCAAATGAAGAACCTTCATTTCTTGATCTTCATCCATGGAGTTACACACATGATGAGAGAAGAGGATCCGAGGTTCCTCTCAGCACAAATTTCTCAACCTTGAGACATAAAACTAACAAGGTTGAAGACTAGTATAGTAATATATTTGCAGCTGACATGATCTCTCTGATCCTAAACTCTCTCACCTTTTCTCTATTTTTTTTTTCAATGTAAGTAAACCAAACCCAATTGACCCCAAAAGAAGAAAAAAACAGCATTTTTCAGCAATAAACAGAAGCCAAAAAGAGATAAGAATAAGATAGTTTTGATCTTTCTAGATCAGAGAAGAAACTGAATTAGAACCAGGTTGATCAGTACTCCAAACACTACYCAAACCTGTTGCTGTTGCTGNTGTGTTGTTGTTGCTGTTGTTGTGATTATTATTCCAATAAAGAGAAGCATTATTATCAGACAAACCCATATTCTCCATCTGGTTTTGAAAAACAGCACCACCATTACCATTCAGATTATTCATATTCCACTGATCCAACCTATTTTGTAGCCCTTCACCTAATTTCACTTCTTTTGAATCCATCTCTTGATGATGATGAAAAGGATCATCATCACCACCACCACCACCACCACCACCTTTTATTCCACCACCAGTACTCATGAACTTCTGTTGCAGTAGTGTAGAGCTAAGTAGAGAAGCCATAACAGGAGTAGTTGAAGTAGAAGAAGAACCAAAGATGGAACTGTAATTGTTATTATTTGAAGTACTAAACCCATTTCTGTAACTACCCTCATCATGATGATGATGATGATGATGATTAAGATTAACTTCTCCTCCAGCAGCAGACATTAGTATCCCAGATGAAAAACCTAATCCAAGACCATTCTGGGGAAGATTATCATAACCAGAAGCAGGATTTGAAACTCTAGTAGTAGAATTAAACCTTGTTGAAAAAGGAAAATTCATAACATCATGACAAGAGTTACCACCATAAAGCATAGTATTGATATGGTTTGAGGTAGCAGCAATATCAAAACTAATGGTGCTGTTGTTGTTGTTGTTGTTGTTGAGATTGAGAAGGAGGGTTTGGATTAGCAACAACACTAGAAGGAGTTGAAGAAGAAGGAGAAGCAGAAGAATCAATGTTGTTGTTGTTGGAACAAGAGTATGTTGGTCGTTTAAGACGTTTGTTTTTTCTGCAACCACCACCAACAGGAACATTTCTGAGTGTTCCACCTCTTGTCCAGTATCTTTTACAGGCTTTGCAGAAGTGTCTCGGTTGCGTTAAACTGTAGTTATTGTAGTAACAGAATTTGGTGTTGGATGAATCACAACGTGGGCATCTTAAAGCTTGTTGTTGTTGTTGAAGAAGCTCTTGACCTGGTTTTTCCATAACCCTATTACCGGTTGAAGAAGCCATTGAAACCTTATGATCATCTATCTCACTCTACAATCAAAAGGATAAGAAATAAAAATGAATAAACTAATTGAAAAACAGAAGAGANACCRTTACCAAACATTTTGGTTTCTATGAGAGTAGTTGGTGCCTTGAGTCTTGTTTGATGGTCTTAGAAATGGAGTGAAGGTGGC
TARGET=966,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05284 Ps_contig_mira-and-tgicl-ass_franssen_37067
SEQUENCE=GTCGCTTGATAGTACTACTCTTTATCATCAATTGTCTAAAGTAGATAATAATAATACAACAACAACCATACACTTTGCATGAACATAAAGTTTCTCACACACCAAAGTAYCAAATCAATCCCACATACATGAATATTAATTAATCACCCCCTCTTCTATACCATTCATTATACCCCAAACCCCAATAATCTCCATAACCTAACCTCTCCCAAATGTTAAGAAAGTTAACAAACATTAGTTTCTTTCATGAGAAAAGAAACTAATGACAATACATTAGAAAAAAGAGTAAAGAAAATAAAGGATATTCATATGTCATTGCATATACACATTACAAAACTAATGAAAATGACCAACTTCAACTTACTCAGTACTCAGTAGTAGTACTCACCAAATGAAGAACCTTCATTTCTTGATCTTCATCCATGGAGTTACACACATGATGAGAGAAGAGGATCCGAGGTTCCTCTCAGCACAAATTTCTCAACCTTGAGACATAAAACTAACAAGGTTGAAGACTAGTATAGTAATATATTTGCAGCTGACATGATCTCTCTGATCCTAAACTCTCTCACCTTTTCTCTATTTTTTTTTTCAATGTAAGTAAACCAAACCCAATTGACCCCAAAAGAAGAAAAAAACAGCATTTTTCAGCAATAAACAGAAGCCAAAAAGAGATAAGAATAAGATAGTTTTGATCTTTCTAGATCAGAGAAGAAACTGAATTAGAACCAGGTTGATCAGTACTCCAAACACTACYCAAACCTGTTGCTGTTGCTGNTGTGTTGTTGTTGCTGTTGTTGTGATTATTATTCCAATAAAGAGAAGCATTATTATCAGACAAACCCATATTCTCCATCTGGTTTTGAAAAACAGCACCACCATTACCATTCAGATTATTCATATTCCACTGATCCAACCTATTTTGTAGCCCTTCACCTAATTTCACTTCTTTTGAATCCATCTCTTGATGATGATGAAAAGGATCATCATCACCACCACCACCACCACCACCACCTTTTATTCCACCACCAGTACTCATGAACTTCTGTTGCAGTAGTGTAGAGCTAAGTAGAGAAGCCATAACAGGAGTAGTTGAAGTAGAAGAAGAACCAAAGATGGAACTGTAATTGTTATTATTTGAAGTACTAAACCCATTTCTGTAACTACCCTCATCATGATGATGATGATGATGATGATTAAGATTAACTTCTCCTCCAGCAGCAGACATTAGTATCCCAGATGAAAAACCTAATCCAAGACCATTCTGGGGAAGATTATCATAACCAGAAGCAGGATTTGAAACTCTAGTAGTAGAATTAAACCTTGTTGAAAAAGGAAAATTCATAACATCATGACAAGAGTTACCACCATAAAGCATAGTATTGATATGGTTTGAGGTAGCAGCAATATCAAAACTAATGGTGCTGTTGTTGTTGTTGTTGTTGTTGAGATTGAGAAGGAGGGTTTGGATTAGCAACAACACTAGAAGGAGTTGAAGAAGAAGGAGAAGCAGAAGAATCAATGTTGTTGTTGTTGGAACAAGAGTATGTTGGTCGTTTAAGACGTTTGTTTTTTCTGCAACCACCACCAACAGGAACATTTCTGAGTGTTCCACCTCTTGTCCAGTATCTTTTACAGGCTTTGCAGAAGTGTCTCGGTTGCGTTAAACTGTAGTTATTGTAGTAACAGAATTTGGTGTTGGATGAATCACAACGTGGGCATCTTAAAGCTTGTTGTTGTTGTTGAAGAAGCTCTTGACCTGGTTTTTCCATAACCCTATTACCGGTTGAAGAAGCCATTGAAACCTTATGATCATCTATCTCACTCTACAATCAAAAGGATAAGAAATAAAAATGAATAAACTAATTGAAAAACAGAAGAGANACCRTTACCAAACATTTTGGTTTCTATGAGAGTAGTTGGTGCCTTGAGTCTTGTTTGATGGTCTTAGAAATGGAGTGAAGGTGGC
TARGET=991,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05285 Ps_contig_mira-and-tgicl-ass_franssen_37067
SEQUENCE=GTCGCTTGATAGTACTACTCTTTATCATCAATTGTCTAAAGTAGATAATAATAATACAACAACAACCATACACTTTGCATGAACATAAAGTTTCTCACACACCAAAGTAYCAAATCAATCCCACATACATGAATATTAATTAATCACCCCCTCTTCTATACCATTCATTATACCCCAAACCCCAATAATCTCCATAACCTAACCTCTCCCAAATGTTAAGAAAGTTAACAAACATTAGTTTCTTTCATGAGAAAAGAAACTAATGACAATACATTAGAAAAAAGAGTAAAGAAAATAAAGGATATTCATATGTCATTGCATATACACATTACAAAACTAATGAAAATGACCAACTTCAACTTACTCAGTACTCAGTAGTAGTACTCACCAAATGAAGAACCTTCATTTCTTGATCTTCATCCATGGAGTTACACACATGATGAGAGAAGAGGATCCGAGGTTCCTCTCAGCACAAATTTCTCAACCTTGAGACATAAAACTAACAAGGTTGAAGACTAGTATAGTAATATATTTGCAGCTGACATGATCTCTCTGATCCTAAACTCTCTCACCTTTTCTCTATTTTTTTTTTCAATGTAAGTAAACCAAACCCAATTGACCCCAAAAGAAGAAAAAAACAGCATTTTTCAGCAATAAACAGAAGCCAAAAAGAGATAAGAATAAGATAGTTTTGATCTTTCTAGATCAGAGAAGAAACTGAATTAGAACCAGGTTGATCAGTACTCCAAACACTACYCAAACCTGTTGCTGTTGCTGNTGTGTTGTTGTTGCTGTTGTTGTGATTATTATTCCAATAAAGAGAAGCATTATTATCAGACAAACCCATATTCTCCATCTGGTTTTGAAAAACAGCACCACCATTACCATTCAGATTATTCATATTCCACTGATCCAACCTATTTTGTAGCCCTTCACCTAATTTCACTTCTTTTGAATCCATCTCTTGATGATGATGAAAAGGATCATCATCACCACCACCACCACCACCACCACCTTTTATTCCACCACCAGTACTCATGAACTTCTGTTGCAGTAGTGTAGAGCTAAGTAGAGAAGCCATAACAGGAGTAGTTGAAGTAGAAGAAGAACCAAAGATGGAACTGTAATTGTTATTATTTGAAGTACTAAACCCATTTCTGTAACTACCCTCATCATGATGATGATGATGATGATGATTAAGATTAACTTCTCCTCCAGCAGCAGACATTAGTATCCCAGATGAAAAACCTAATCCAAGACCATTCTGGGGAAGATTATCATAACCAGAAGCAGGATTTGAAACTCTAGTAGTAGAATTAAACCTTGTTGAAAAAGGAAAATTCATAACATCATGACAAGAGTTACCACCATAAAGCATAGTATTGATATGGTTTGAGGTAGCAGCAATATCAAAACTAATGGTGCTGTTGTTGTTGTTGTTGTTGTTGAGATTGAGAAGGAGGGTTTGGATTAGCAACAACACTAGAAGGAGTTGAAGAAGAAGGAGAAGCAGAAGAATCAATGTTGTTGTTGTTGGAACAAGAGTATGTTGGTCGTTTAAGACGTTTGTTTTTTCTGCAACCACCACCAACAGGAACATTTCTGAGTGTTCCACCTCTTGTCCAGTATCTTTTACAGGCTTTGCAGAAGTGTCTCGGTTGCGTTAAACTGTAGTTATTGTAGTAACAGAATTTGGTGTTGGATGAATCACAACGTGGGCATCTTAAAGCTTGTTGTTGTTGTTGAAGAAGCTCTTGACCTGGTTTTTCCATAACCCTATTACCGGTTGAAGAAGCCATTGAAACCTTATGATCATCTATCTCACTCTACAATCAAAAGGATAAGAAATAAAAATGAATAAACTAATTGAAAAACAGAAGAGANACCRTTACCAAACATTTTGGTTTCTATGAGAGTAGTTGGTGCCTTGAGTCTTGTTTGATGGTCTTAGAAATGGAGTGAAGGTGGC
TARGET=1175,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05286 Ps_contig_mira-and-tgicl-ass_franssen_37067
SEQUENCE=GTCGCTTGATAGTACTACTCTTTATCATCAATTGTCTAAAGTAGATAATAATAATACAACAACAACCATACACTTTGCATGAACATAAAGTTTCTCACACACCAAAGTAYCAAATCAATCCCACATACATGAATATTAATTAATCACCCCCTCTTCTATACCATTCATTATACCCCAAACCCCAATAATCTCCATAACCTAACCTCTCCCAAATGTTAAGAAAGTTAACAAACATTAGTTTCTTTCATGAGAAAAGAAACTAATGACAATACATTAGAAAAAAGAGTAAAGAAAATAAAGGATATTCATATGTCATTGCATATACACATTACAAAACTAATGAAAATGACCAACTTCAACTTACTCAGTACTCAGTAGTAGTACTCACCAAATGAAGAACCTTCATTTCTTGATCTTCATCCATGGAGTTACACACATGATGAGAGAAGAGGATCCGAGGTTCCTCTCAGCACAAATTTCTCAACCTTGAGACATAAAACTAACAAGGTTGAAGACTAGTATAGTAATATATTTGCAGCTGACATGATCTCTCTGATCCTAAACTCTCTCACCTTTTCTCTATTTTTTTTTTCAATGTAAGTAAACCAAACCCAATTGACCCCAAAAGAAGAAAAAAACAGCATTTTTCAGCAATAAACAGAAGCCAAAAAGAGATAAGAATAAGATAGTTTTGATCTTTCTAGATCAGAGAAGAAACTGAATTAGAACCAGGTTGATCAGTACTCCAAACACTACYCAAACCTGTTGCTGTTGCTGNTGTGTTGTTGTTGCTGTTGTTGTGATTATTATTCCAATAAAGAGAAGCATTATTATCAGACAAACCCATATTCTCCATCTGGTTTTGAAAAACAGCACCACCATTACCATTCAGATTATTCATATTCCACTGATCCAACCTATTTTGTAGCCCTTCACCTAATTTCACTTCTTTTGAATCCATCTCTTGATGATGATGAAAAGGATCATCATCACCACCACCACCACCACCACCACCTTTTATTCCACCACCAGTACTCATGAACTTCTGTTGCAGTAGTGTAGAGCTAAGTAGAGAAGCCATAACAGGAGTAGTTGAAGTAGAAGAAGAACCAAAGATGGAACTGTAATTGTTATTATTTGAAGTACTAAACCCATTTCTGTAACTACCCTCATCATGATGATGATGATGATGATGATTAAGATTAACTTCTCCTCCAGCAGCAGACATTAGTATCCCAGATGAAAAACCTAATCCAAGACCATTCTGGGGAAGATTATCATAACCAGAAGCAGGATTTGAAACTCTAGTAGTAGAATTAAACCTTGTTGAAAAAGGAAAATTCATAACATCATGACAAGAGTTACCACCATAAAGCATAGTATTGATATGGTTTGAGGTAGCAGCAATATCAAAACTAATGGTGCTGTTGTTGTTGTTGTTGTTGTTGAGATTGAGAAGGAGGGTTTGGATTAGCAACAACACTAGAAGGAGTTGAAGAAGAAGGAGAAGCAGAAGAATCAATGTTGTTGTTGTTGGAACAAGAGTATGTTGGTCGTTTAAGACGTTTGTTTTTTCTGCAACCACCACCAACAGGAACATTTCTGAGTGTTCCACCTCTTGTCCAGTATCTTTTACAGGCTTTGCAGAAGTGTCTCGGTTGCGTTAAACTGTAGTTATTGTAGTAACAGAATTTGGTGTTGGATGAATCACAACGTGGGCATCTTAAAGCTTGTTGTTGTTGTTGAAGAAGCTCTTGACCTGGTTTTTCCATAACCCTATTACCGGTTGAAGAAGCCATTGAAACCTTATGATCATCTATCTCACTCTACAATCAAAAGGATAAGAAATAAAAATGAATAAACTAATTGAAAAACAGAAGAGANACCRTTACCAAACATTTTGGTTTCTATGAGAGTAGTTGGTGCCTTGAGTCTTGTTTGATGGTCTTAGAAATGGAGTGAAGGTGGC
TARGET=1426,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05287 Ps_contig_mira-and-tgicl-ass_franssen_37067
SEQUENCE=GTCGCTTGATAGTACTACTCTTTATCATCAATTGTCTAAAGTAGATAATAATAATACAACAACAACCATACACTTTGCATGAACATAAAGTTTCTCACACACCAAAGTAYCAAATCAATCCCACATACATGAATATTAATTAATCACCCCCTCTTCTATACCATTCATTATACCCCAAACCCCAATAATCTCCATAACCTAACCTCTCCCAAATGTTAAGAAAGTTAACAAACATTAGTTTCTTTCATGAGAAAAGAAACTAATGACAATACATTAGAAAAAAGAGTAAAGAAAATAAAGGATATTCATATGTCATTGCATATACACATTACAAAACTAATGAAAATGACCAACTTCAACTTACTCAGTACTCAGTAGTAGTACTCACCAAATGAAGAACCTTCATTTCTTGATCTTCATCCATGGAGTTACACACATGATGAGAGAAGAGGATCCGAGGTTCCTCTCAGCACAAATTTCTCAACCTTGAGACATAAAACTAACAAGGTTGAAGACTAGTATAGTAATATATTTGCAGCTGACATGATCTCTCTGATCCTAAACTCTCTCACCTTTTCTCTATTTTTTTTTTCAATGTAAGTAAACCAAACCCAATTGACCCCAAAAGAAGAAAAAAACAGCATTTTTCAGCAATAAACAGAAGCCAAAAAGAGATAAGAATAAGATAGTTTTGATCTTTCTAGATCAGAGAAGAAACTGAATTAGAACCAGGTTGATCAGTACTCCAAACACTACYCAAACCTGTTGCTGTTGCTGNTGTGTTGTTGTTGCTGTTGTTGTGATTATTATTCCAATAAAGAGAAGCATTATTATCAGACAAACCCATATTCTCCATCTGGTTTTGAAAAACAGCACCACCATTACCATTCAGATTATTCATATTCCACTGATCCAACCTATTTTGTAGCCCTTCACCTAATTTCACTTCTTTTGAATCCATCTCTTGATGATGATGAAAAGGATCATCATCACCACCACCACCACCACCACCACCTTTTATTCCACCACCAGTACTCATGAACTTCTGTTGCAGTAGTGTAGAGCTAAGTAGAGAAGCCATAACAGGAGTAGTTGAAGTAGAAGAAGAACCAAAGATGGAACTGTAATTGTTATTATTTGAAGTACTAAACCCATTTCTGTAACTACCCTCATCATGATGATGATGATGATGATGATTAAGATTAACTTCTCCTCCAGCAGCAGACATTAGTATCCCAGATGAAAAACCTAATCCAAGACCATTCTGGGGAAGATTATCATAACCAGAAGCAGGATTTGAAACTCTAGTAGTAGAATTAAACCTTGTTGAAAAAGGAAAATTCATAACATCATGACAAGAGTTACCACCATAAAGCATAGTATTGATATGGTTTGAGGTAGCAGCAATATCAAAACTAATGGTGCTGTTGTTGTTGTTGTTGTTGTTGAGATTGAGAAGGAGGGTTTGGATTAGCAACAACACTAGAAGGAGTTGAAGAAGAAGGAGAAGCAGAAGAATCAATGTTGTTGTTGTTGGAACAAGAGTATGTTGGTCGTTTAAGACGTTTGTTTTTTCTGCAACCACCACCAACAGGAACATTTCTGAGTGTTCCACCTCTTGTCCAGTATCTTTTACAGGCTTTGCAGAAGTGTCTCGGTTGCGTTAAACTGTAGTTATTGTAGTAACAGAATTTGGTGTTGGATGAATCACAACGTGGGCATCTTAAAGCTTGTTGTTGTTGTTGAAGAAGCTCTTGACCTGGTTTTTCCATAACCCTATTACCGGTTGAAGAAGCCATTGAAACCTTATGATCATCTATCTCACTCTACAATCAAAAGGATAAGAAATAAAAATGAATAAACTAATTGAAAAACAGAAGAGANACCRTTACCAAACATTTTGGTTTCTATGAGAGTAGTTGGTGCCTTGAGTCTTGTTTGATGGTCTTAGAAATGGAGTGAAGGTGGC
TARGET=1523,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05288 Ps_contig_mira-and-tgicl-ass_franssen_37067
SEQUENCE=GTCGCTTGATAGTACTACTCTTTATCATCAATTGTCTAAAGTAGATAATAATAATACAACAACAACCATACACTTTGCATGAACATAAAGTTTCTCACACACCAAAGTAYCAAATCAATCCCACATACATGAATATTAATTAATCACCCCCTCTTCTATACCATTCATTATACCCCAAACCCCAATAATCTCCATAACCTAACCTCTCCCAAATGTTAAGAAAGTTAACAAACATTAGTTTCTTTCATGAGAAAAGAAACTAATGACAATACATTAGAAAAAAGAGTAAAGAAAATAAAGGATATTCATATGTCATTGCATATACACATTACAAAACTAATGAAAATGACCAACTTCAACTTACTCAGTACTCAGTAGTAGTACTCACCAAATGAAGAACCTTCATTTCTTGATCTTCATCCATGGAGTTACACACATGATGAGAGAAGAGGATCCGAGGTTCCTCTCAGCACAAATTTCTCAACCTTGAGACATAAAACTAACAAGGTTGAAGACTAGTATAGTAATATATTTGCAGCTGACATGATCTCTCTGATCCTAAACTCTCTCACCTTTTCTCTATTTTTTTTTTCAATGTAAGTAAACCAAACCCAATTGACCCCAAAAGAAGAAAAAAACAGCATTTTTCAGCAATAAACAGAAGCCAAAAAGAGATAAGAATAAGATAGTTTTGATCTTTCTAGATCAGAGAAGAAACTGAATTAGAACCAGGTTGATCAGTACTCCAAACACTACYCAAACCTGTTGCTGTTGCTGNTGTGTTGTTGTTGCTGTTGTTGTGATTATTATTCCAATAAAGAGAAGCATTATTATCAGACAAACCCATATTCTCCATCTGGTTTTGAAAAACAGCACCACCATTACCATTCAGATTATTCATATTCCACTGATCCAACCTATTTTGTAGCCCTTCACCTAATTTCACTTCTTTTGAATCCATCTCTTGATGATGATGAAAAGGATCATCATCACCACCACCACCACCACCACCACCTTTTATTCCACCACCAGTACTCATGAACTTCTGTTGCAGTAGTGTAGAGCTAAGTAGAGAAGCCATAACAGGAGTAGTTGAAGTAGAAGAAGAACCAAAGATGGAACTGTAATTGTTATTATTTGAAGTACTAAACCCATTTCTGTAACTACCCTCATCATGATGATGATGATGATGATGATTAAGATTAACTTCTCCTCCAGCAGCAGACATTAGTATCCCAGATGAAAAACCTAATCCAAGACCATTCTGGGGAAGATTATCATAACCAGAAGCAGGATTTGAAACTCTAGTAGTAGAATTAAACCTTGTTGAAAAAGGAAAATTCATAACATCATGACAAGAGTTACCACCATAAAGCATAGTATTGATATGGTTTGAGGTAGCAGCAATATCAAAACTAATGGTGCTGTTGTTGTTGTTGTTGTTGTTGAGATTGAGAAGGAGGGTTTGGATTAGCAACAACACTAGAAGGAGTTGAAGAAGAAGGAGAAGCAGAAGAATCAATGTTGTTGTTGTTGGAACAAGAGTATGTTGGTCGTTTAAGACGTTTGTTTTTTCTGCAACCACCACCAACAGGAACATTTCTGAGTGTTCCACCTCTTGTCCAGTATCTTTTACAGGCTTTGCAGAAGTGTCTCGGTTGCGTTAAACTGTAGTTATTGTAGTAACAGAATTTGGTGTTGGATGAATCACAACGTGGGCATCTTAAAGCTTGTTGTTGTTGTTGAAGAAGCTCTTGACCTGGTTTTTCCATAACCCTATTACCGGTTGAAGAAGCCATTGAAACCTTATGATCATCTATCTCACTCTACAATCAAAAGGATAAGAAATAAAAATGAATAAACTAATTGAAAAACAGAAGAGANACCRTTACCAAACATTTTGGTTTCTATGAGAGTAGTTGGTGCCTTGAGTCTTGTTTGATGGTCTTAGAAATGGAGTGAAGGTGGC
TARGET=1731,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05289 Ps_contig_mira-and-tgicl-ass_franssen_37071
SEQUENCE=ACAAGGAATGCAGCACACTGCATCATCTGCACTCGTTACTGTTCCCGAAGAAGGCCTCACACAGACTCCTCCGATGGAGCTTGTTGGTCATTCAATTGCATGTGCTACGAATCCCTATCAGGATCCATATTATGGGGGGATGATGGCAGCTTATGGTCACCAGCAGTTGGCATACCATCCTTTTATAGGAATGCCTCATCCCAGAATGCCTTTGCCCCTCGAGATGACTCAGGAACCTGTTTATGTGAATGCCAAGCAATATCAAGGAATTCTGAGGCGAAGACAAGCTCGTGCTAAAGCAGAGCTTGAAAGGAAGCTCATAAAATCTAGAAAGCCATATCTTCATGAATCTAGACATCAGCATGCTATGAGAAGGGCAAGAGGCACAGGAGGACGATTTGCAAAGAAAACTGATGGCGAAGGCTCAAACAACTCAGGAAAGGATAATGGCTCTGGTCCAGTCCTGTCATCACAGTCAATTAGTTCATCTGGTTCAGAACCCTTGCCTTCAGACTCTGCGGAAACCTGGAATTCTCCCAACATGCGACAAGATGCAAGAGGATCAAATGAAAACGGTGGTGTCTCCTACCATAATAACAACAATGGTATGCAATCTTCAAGATATCAAGGTGAAAGAGTTGATGAAGGGGACTGTTCGGGGCAACTACGCGGAAGCATCTCCTCGAACGAGGCATCGCAGAGACGCCTAGCTATTCAGTAGACCCCTCCCCTGCAAGGGGAAGATAGATGCTGAGGTTGGTTTTGTATCCTAGTGATATATATTTGGTGAATGAATAGCTTACCTTGGTTATCAGTGAACATTCTTGGCAATATATATGTCAAGCGGCAAATCATTCTTGGCTTTTGTTTATGGTGTGAATGAATGATATAATATGGGGGGAGAAGAATGATGATTGGGGTAAGTGATTTCTGTTGAAGTCCCGAGCAATCAATCCTTCATTCTTTTCTCATTTTTGCATTTTGTACAGAGTTTTAGTGGGTAGTCAAGTTCAGCTGATATGGATGGATGGTCAGGTCAGAAGACTGGAGAAACAAACAAACACTTTTGATGTACCAACTCTCTTGTTTGACGGGTTTATTTTGTGCAACTTATCCAGTTGGTTGTGTTAATGTTAACATGGGATTTCATTATAATAGAATCAAACATGCATCAAGTTCTTARTCACG
TARGET=1020,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05290 Ps_contig_mira-and-tgicl-ass_franssen_37071
SEQUENCE=ACAAGGAATGCAGCACACTGCATCATCTGCACTCGTTACTGTTCCCGAAGAAGGCCTCACACAGACTCCTCCGATGGAGCTTGTTGGTCATTCAATTGCATGTGCTACGAATCCCTATCAGGATCCATATTATGGGGGGATGATGGCAGCTTATGGTCACCAGCAGTTGGCATACCATCCTTTTATAGGAATGCCTCATCCCAGAATGCCTTTGCCCCTCGAGATGACTCAGGAACCTGTTTATGTGAATGCCAAGCAATATCAAGGAATTCTGAGGCGAAGACAAGCTCGTGCTAAAGCAGAGCTTGAAAGGAAGCTCATAAAATCTAGAAAGCCATATCTTCATGAATCTAGACATCAGCATGCTATGAGAAGGGCAAGAGGCACAGGAGGACGATTTGCAAAGAAAACTGATGGCGAAGGCTCAAACAACTCAGGAAAGGATAATGGCTCTGGTCCAGTCCTGTCATCACAGTCAATTAGTTCATCTGGTTCAGAACCCTTGCCTTCAGACTCTGCGGAAACCTGGAATTCTCCCAACATGCGACAAGATGCAAGAGGATCAAATGAAAACGGTGGTGTCTCCTACCATAATAACAACAATGGTATGCAATCTTCAAGATATCAAGGTGAAAGAGTTGATGAAGGGGACTGTTCGGGGCAACTACGCGGAAGCATCTCCTCGAACGAGGCATCGCAGAGACGCCTAGCTATTCAGTAGACCCCTCCCCTGCAAGGGGAAGATAGATGCTGAGGTTGGTTTTGTATCCTAGTGATATATATTTGGTGAATGAATAGCTTACCTTGGTTATCAGTGAACATTCTTGGCAATATATATGTCAAGCGGCAAATCATTCTTGGCTTTTGTTTATGGTGTGAATGAATGATATAATATGGGGGGAGAAGAATGATGATTGGGGTAAGTGATTTCTGTTGAAGTCCCGAGCAATCAATCCTTCATTCTTTTCTCATTTTTGCATTTTGTACAGAGTTTTAGTGGGTAGTCAAGTTCAGCTGATATGGATGGATGGTCAGGTCAGAAGACTGGAGAAACAAACAAACACTTTTGATGTACCAACTCTCTTGTTTGACGGGTTTATTTTGTGCAACTTATCCAGTTGGTTGTGTTAATGTTAACATGGGATTTCATTATAATAGAATCAAACATGCATCAAGTTCTTARTCACG
TARGET=1051,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05291 Ps_contig_mira-and-tgicl-ass_franssen_37076
SEQUENCE=TTAAAATATTGAAAAAATAAGTTCTTATAAGTTATTAGACATCTCAATAAATAACTCNCCCTTCCTGTCATAATCACTTCCCTACTAGGAAGTAGCTACTTGCAATGGAAGATCTTGTGTGCAATGGCATAAGTGCAAAATTGATTATGCCTTCACACTATACTCTACAAGTAGGCAAATAAAAACCATATCATGCCATTAATCAGGTTCCATAACTCACCCAAACTAAATCATATATAGATGAAGCGTGGTTTAAATTCATGTTCTTGTATAAACTTAAACAACCTTCCAGGTAACATTCAACAAAAAGTAATAAAAAAACAAAATTGAGGGCACAGTAAAGCCAGCCAGCCAACCAAAGTTTTCGACATCCACTACTTCCGCTTTGAAGAGTAGTGATTGTCATTTGGATTTCTCTTCCCACTGTAAACTGCTAGTGCTTTTCTGACTGGAGGCAGGAACGGAGATCCCTTAGAATTGTGGTGGCTGAAAACATCGTCTGGCCCAAAGAACTCATCATCCTTGTTGGCTTTCTTTCTTGTTTTAGGATGAAGTACCCTTTGAGGCAGTTGATCTTCCCGGAGAATAACATTTTTTGTGACAGCATTCCTTCCGCCTTGGGGTAAAGGCAATGAGAATTTTGTGCCACGTAATGTAACTCGGGGTCGACGGGCTGCCAACATTATTCCATTTTCATTAACAGTGACAGCTACCTTCCTTAAGGTGCCACCATTTCCACATTTTGGACAGAAAATCCTCCCAATTTCAGCAGTAACAGTGAAGCAAGCGTGACACTTAAGTATCCATCTGTGCAGCTGGTGTATCTGTGATCCCCCAGGTGCCAGCAAGCGCAAACCCATTTGGAGAAGAACATTTTGCATTGCAAAGTCGCCAGTTATACAGGCTACAGATGATTCAAGAACAAAGNTCTAAGCAATACCTAACTTT
TARGET=342,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0708
PRIMER_LEFT_PENALTY=0.047267
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=ATCATGCCATTAATCAGGTTCC
PRIMER_RIGHT_SEQUENCE=ACAGTGGGAAGAGAAATCCAAA
PRIMER_LEFT=189,22
PRIMER_RIGHT=426,22
PRIMER_LEFT_TM=60.047
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.0931
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.093026
PRIMER_LEFT_1_SEQUENCE=ATGGAAGATCTTGTGTGCAATG
PRIMER_RIGHT_1_SEQUENCE=AGTGGATGTCGAAAACTTTGGT
PRIMER_LEFT_1=104,22
PRIMER_RIGHT_1=375,22
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=59.907
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.1403
PRIMER_LEFT_2_PENALTY=0.047267
PRIMER_RIGHT_2_PENALTY=0.093026
PRIMER_LEFT_2_SEQUENCE=ATCATGCCATTAATCAGGTTCC
PRIMER_RIGHT_2_SEQUENCE=AGTGGATGTCGAAAACTTTGGT
PRIMER_LEFT_2=189,22
PRIMER_RIGHT_2=375,22
PRIMER_LEFT_2_TM=60.047
PRIMER_RIGHT_2_TM=59.907
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=0.2166
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=0.216588
PRIMER_LEFT_3_SEQUENCE=ATGGAAGATCTTGTGTGCAATG
PRIMER_RIGHT_3_SEQUENCE=CTTCAAAGCGGAAGTAGTGGAT
PRIMER_LEFT_3=104,22
PRIMER_RIGHT_3=390,22
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=59.783
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=0.2356
PRIMER_LEFT_4_PENALTY=0.047267
PRIMER_RIGHT_4_PENALTY=0.188292
PRIMER_LEFT_4_SEQUENCE=ATCATGCCATTAATCAGGTTCC
PRIMER_RIGHT_4_SEQUENCE=AATTCTAAGGGATCTCCGTTCC
PRIMER_LEFT_4=189,22
PRIMER_RIGHT_4=478,22
PRIMER_LEFT_4_TM=60.047
PRIMER_RIGHT_4_TM=59.812
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_05292 Ps_contig_mira-and-tgicl-ass_franssen_37078
SEQUENCE=ATCTACTACCGAACCGAATTCCAAATANACATGTAAGGGAGTTTGGTAGAAAAGATGACGATATATGCACACGCATGAGCTGTAACTAACAAGAGCAAACGAGACGGCGGCAGGAGAGTGACGGAAAAAAATGGGTTACAACATAATGAATTTCTCCGCCGCTTCATCATCGTTAATCGGAACTCAAACTAACAATATCCACAACAATAAGCTACAAAAAGCGCGTTCATCTTCTTCTTCTTCATTTTCACTTAACACTACTCGCCACCGCCACTACCACCGACAACTACACCTTCATTTTCAATCTTCCGATGTCATTCCTCTCCGTTCATCCTATACGCGATCCCTTTCTCATTCTTCTATGACTGTGAAGGCGGTGGCGCAGACGGAGGAGCCGCTACGTGTTATGATTTCTGGTGCTCCTGCTTCTGGTAAAGGAACTCAATGCCAACTTATTACCAAGAAATATGATTTGGTGCATGTTGCTGCTGGAGATTTACTTAGGGCGGAAATTGAAACCGGAAGTGAAAATGGAAAGCGTGCAAAAGAATATATGGAGAAGGGACAATTGGTCCCTGATGAAATAGTTGTCATGATGGTCAAGGATCGTCTCTTGAAGCCAGATTCAGTAGAGAATGGTTGGCTTTTGGATGGATATCCCAGGAGCTTATCGCAGGCTATTGCACTTAAGGAATTAGGGTTTGAACCTGATATTTTTATTCTTCTAGAGGTCTCTGAAGATATTCTTGTGGAGAGAGTAGTTGGAAGGAGATTAGATCCTGTTACTGGGAAAATATACCATTTGAAGTATTCTCCTCCAGAAACAAAAGAAATTGCAGATAGGCTTACTCAACGTTTTGACGATACTGAAGAGAAGGTAAAGTTGCGACTGAACACCCATCATCAAAATGTGGAGGCAGTCCTTTCCATGTTTAAAGATATAACCATTAAGATTAATGGAAATGCCTCTCAGGAGGATGTATTTACTCAAATTGATGCCTCACTTACAACTCTTTTAGAGCAAAGGAAATCATCTTCAGAATCAATGGTAGCATAGAAGATCATCGTTTGACATTTTGATGTACCATGGGGCCTTAATGGAGGTAATGGATTTAAAACTACATATTTATTTTTTTATTCAGTTAAAAGAGTCAAAATAAGCCTCCTTTAATCGATGAGTTTGATAGTACAGTGTTGTATTGGACGTCATTTTCAGTGATAGTAAACTCCGTCTTAAATTTTACAAGTTCATTTTAGTGAGAAAATGGAGGATGTAGTGAGTCGTAGTTTGTTGGTATGATTCCTCCTGTTATTGCTGGTCTGTATGTTGTGTTCTTTACCATTTTCTGTATATTATCGTTGGAAATTATGTATTTGATTTATGAAGGAAAATAAAAAATAAAGGAAAATCATCCATTCAATGTTAA
TARGET=230,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3525
PRIMER_LEFT_PENALTY=0.016830
PRIMER_RIGHT_PENALTY=0.335710
PRIMER_LEFT_SEQUENCE=AGATGACGATATATGCACACGC
PRIMER_RIGHT_SEQUENCE=ATGAACGGAGAGGAATGACATC
PRIMER_LEFT=52,22
PRIMER_RIGHT=331,22
PRIMER_LEFT_TM=60.017
PRIMER_RIGHT_TM=60.336
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.3714
PRIMER_LEFT_1_PENALTY=0.040146
PRIMER_RIGHT_1_PENALTY=0.331254
PRIMER_LEFT_1_SEQUENCE=CATCATCGTTAATCGGAACTCA
PRIMER_RIGHT_1_SEQUENCE=CAGAAATCATAACACGTAGCGG
PRIMER_LEFT_1=164,22
PRIMER_RIGHT_1=415,22
PRIMER_LEFT_1_TM=59.960
PRIMER_RIGHT_1_TM=59.669
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.3714
PRIMER_LEFT_2_PENALTY=0.040146
PRIMER_RIGHT_2_PENALTY=0.331254
PRIMER_LEFT_2_SEQUENCE=CATCATCGTTAATCGGAACTCA
PRIMER_RIGHT_2_SEQUENCE=CCAGAAATCATAACACGTAGCG
PRIMER_LEFT_2=164,22
PRIMER_RIGHT_2=416,22
PRIMER_LEFT_2_TM=59.960
PRIMER_RIGHT_2_TM=59.669
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.3759
PRIMER_LEFT_3_PENALTY=0.040146
PRIMER_RIGHT_3_PENALTY=0.335710
PRIMER_LEFT_3_SEQUENCE=CATCATCGTTAATCGGAACTCA
PRIMER_RIGHT_3_SEQUENCE=ATGAACGGAGAGGAATGACATC
PRIMER_LEFT_3=164,22
PRIMER_RIGHT_3=331,22
PRIMER_LEFT_3_TM=59.960
PRIMER_RIGHT_3_TM=60.336
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=0.4029
PRIMER_LEFT_4_PENALTY=0.040146
PRIMER_RIGHT_4_PENALTY=0.362762
PRIMER_LEFT_4_SEQUENCE=CATCATCGTTAATCGGAACTCA
PRIMER_RIGHT_4_SEQUENCE=GAGCACCAGAAATCATAACACG
PRIMER_LEFT_4=164,22
PRIMER_RIGHT_4=421,22
PRIMER_LEFT_4_TM=59.960
PRIMER_RIGHT_4_TM=59.637
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_05293 Ps_contig_mira-and-tgicl-ass_franssen_37084
SEQUENCE=ACAAATGAAAAGCTAAGTGCATTGGAGAAAGAGGTTGAAACTTTGATAGCTACGGATGAAAGTAAGGGATCTAGTAAAAGTTCTTTAGCATTTCTTGAATTAAAGGATGTGATGGAAAAGCACGAGAAAATGGAGAACGACTCAATTGCTTGTTCGTTGGCCTCCAAGAAAACGAAGGATTTTTCGTCGGAACCAGACTATTCTTGGATTGAAGGTGATAAAGCCGAGCCGTGGTGGCGGATGGCAGATAGAGATGAGTTAGCTTCCTTTGTTTCGCACAAATCATTCAACCATATTGAGAATTGTGATCTTCCTCCTCCTAAGAAGAAGCATCTTAGTGGATACTCTTGTTCTAGTGTTATTACTAATGATGACAAAAAAAAAGAACTGCATCTTATAACTTGGAGGCCGAATCCGGAAGGTTTTCCAATTCGAAGAAGGGTCCTTCTGCCAATGAAGGACTCTTGTATTTTGCTTCTGCCAAATCTTCAAGTGATACCGCTATACTCGACGATGTCAAGCAAAGTATACAAATTTCTGAGGGAGACACTAGCAAAGCTCAATTAATGGAGGCATTATGTCACTCTCAAACACGCGCACGGGAAGCGGAGGAAGCAGCGAAACAAGCATATGCGGAGAAAGAGCACATTGTTACACTCATTTTCAAACAGGCTTCACAACTCTTTGCCTATAAGCAATTGATTAAACTGCTGCAGCTGGAAACACTCTGCAATCAGATCAAGAATAATGATCAGTCAACCTCAACTCTCTTTCCGGTTGCTCTTCCGTGGACGTCGGATGAACGTAGGAAATCACGGAAGAGAAAGCAGACATATTCCAGTGGGCGACGCGACAGAAAAGGCAAGGGGAATTGTGATATTACAACGTATGCGGTTGCGATTGCTTTAGGATTAAGTCTTGTTGGAGCAGGATTGCTATTAGGATGGACTGTGGGTTGGATGTCACCATGTTCATAGTTCTGTTATATATATATATATATATTTGTATCATAGTGTTGATTGTTGCAAGTTCTGAGATCTATCTGTATTTTTGTAGATTAGTTAGTCCATAAACTGTACATTTTGCTTTGTAATTTTGTATTGAATAGAGATTATGTATGTAAATCTTTCTGTGATGATGATGATGAAAAAACACCTAGGATTGTATTTATGATNATNAAGAATGTGTTTACC
TARGET=984,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7715
PRIMER_LEFT_PENALTY=0.108206
PRIMER_RIGHT_PENALTY=1.663339
PRIMER_LEFT_SEQUENCE=AATCACGGAAGAGAAAGCAGAC
PRIMER_RIGHT_SEQUENCE=CAGAACTTGCAACAATCAACAC
PRIMER_LEFT=810,22
PRIMER_RIGHT=1033,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=58.337
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=1.7937
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=1.663339
PRIMER_LEFT_1_SEQUENCE=ACGTAGGAAATCACGGAAGAGA
PRIMER_RIGHT_1_SEQUENCE=CAGAACTTGCAACAATCAACAC
PRIMER_LEFT_1=802,22
PRIMER_RIGHT_1=1033,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=58.337
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=1.9213
PRIMER_LEFT_2_PENALTY=0.257927
PRIMER_RIGHT_2_PENALTY=1.663339
PRIMER_LEFT_2_SEQUENCE=TGTTGGAGCAGGATTGCTATTA
PRIMER_RIGHT_2_SEQUENCE=CAGAACTTGCAACAATCAACAC
PRIMER_LEFT_2=919,22
PRIMER_RIGHT_2=1033,22
PRIMER_LEFT_2_TM=59.742
PRIMER_RIGHT_2_TM=58.337
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=115
PRIMER_PAIR_PENALTY_3=1.9444
PRIMER_LEFT_3_PENALTY=0.281044
PRIMER_RIGHT_3_PENALTY=1.663339
PRIMER_LEFT_3_SEQUENCE=GTCAACCTCAACTCTCTTTCCG
PRIMER_RIGHT_3_SEQUENCE=CAGAACTTGCAACAATCAACAC
PRIMER_LEFT_3=754,22
PRIMER_RIGHT_3=1033,22
PRIMER_LEFT_3_TM=60.281
PRIMER_RIGHT_3_TM=58.337
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=2.0105
PRIMER_LEFT_4_PENALTY=0.347129
PRIMER_RIGHT_4_PENALTY=1.663339
PRIMER_LEFT_4_SEQUENCE=AACGTAGGAAATCACGGAAGAG
PRIMER_RIGHT_4_SEQUENCE=CAGAACTTGCAACAATCAACAC
PRIMER_LEFT_4=801,22
PRIMER_RIGHT_4=1033,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=58.337
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_05294 Ps_contig_mira-and-tgicl-ass_franssen_37084
SEQUENCE=ACAAATGAAAAGCTAAGTGCATTGGAGAAAGAGGTTGAAACTTTGATAGCTACGGATGAAAGTAAGGGATCTAGTAAAAGTTCTTTAGCATTTCTTGAATTAAAGGATGTGATGGAAAAGCACGAGAAAATGGAGAACGACTCAATTGCTTGTTCGTTGGCCTCCAAGAAAACGAAGGATTTTTCGTCGGAACCAGACTATTCTTGGATTGAAGGTGATAAAGCCGAGCCGTGGTGGCGGATGGCAGATAGAGATGAGTTAGCTTCCTTTGTTTCGCACAAATCATTCAACCATATTGAGAATTGTGATCTTCCTCCTCCTAAGAAGAAGCATCTTAGTGGATACTCTTGTTCTAGTGTTATTACTAATGATGACAAAAAAAAAGAACTGCATCTTATAACTTGGAGGCCGAATCCGGAAGGTTTTCCAATTCGAAGAAGGGTCCTTCTGCCAATGAAGGACTCTTGTATTTTGCTTCTGCCAAATCTTCAAGTGATACCGCTATACTCGACGATGTCAAGCAAAGTATACAAATTTCTGAGGGAGACACTAGCAAAGCTCAATTAATGGAGGCATTATGTCACTCTCAAACACGCGCACGGGAAGCGGAGGAAGCAGCGAAACAAGCATATGCGGAGAAAGAGCACATTGTTACACTCATTTTCAAACAGGCTTCACAACTCTTTGCCTATAAGCAATTGATTAAACTGCTGCAGCTGGAAACACTCTGCAATCAGATCAAGAATAATGATCAGTCAACCTCAACTCTCTTTCCGGTTGCTCTTCCGTGGACGTCGGATGAACGTAGGAAATCACGGAAGAGAAAGCAGACATATTCCAGTGGGCGACGCGACAGAAAAGGCAAGGGGAATTGTGATATTACAACGTATGCGGTTGCGATTGCTTTAGGATTAAGTCTTGTTGGAGCAGGATTGCTATTAGGATGGACTGTGGGTTGGATGTCACCATGTTCATAGTTCTGTTATATATATATATATATATTTGTATCATAGTGTTGATTGTTGCAAGTTCTGAGATCTATCTGTATTTTTGTAGATTAGTTAGTCCATAAACTGTACATTTTGCTTTGTAATTTTGTATTGAATAGAGATTATGTATGTAAATCTTTCTGTGATGATGATGATGAAAAAACACCTAGGATTGTATTTATGATNATNAAGAATGTGTTTACC
TARGET=1133,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05295 Ps_contig_mira-and-tgicl-ass_franssen_37086
SEQUENCE=CACCTTCCTCCTGTTCTTCAGTTCCATGCTTATTTTCTTCTTCATCCTGTTCTTCTCTTTCTTCAGTTTCATTCTTGTTCCCTTCTTGTTGTTCTTCACCATGTTCACGTTTATTTTCCGCATCCTCTACAACAGGTTCATCTTCCTCTTCTTCGTGCTTTCCGTTCTGATCTACTTCATCCTTAACCGGATGAAGATCCTTTCTACCGAGTTTCACAACCTTATCCGTCTCAGTTCTGACCGATGATAGTGTTGAATCATTCTTATCGAACTCTTTCTTCTTGTCGTGATTGTGCTTAACCTGGTAAATCAACCAGAAGCACACACCAAGCAGCAAAATAACTTGCAGAACATGCTTTATCTTAACACCTTTGGATCTTTGATTCCTACTACTCGGAAACCTTTTTATCATGATTAACTTAGTTAAAAAAATAAAACGAACTCGTCTTCTCCAAATCGCAAGATTAAGCCACCTGAATGAGAAGAGAAATGGAAAAGGAAAGAAGTAAAGAGGAAGAAGAAGTAGAGTTTGAAGAGAGAGATTCTAGAGAGAGAGAATATAGATATAGGTGTTAGAAATAGAACTTGTTTTTTTTTATGGACTTGAACA
TARGET=547,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05296 Ps_contig_mira-and-tgicl-ass_franssen_37088
SEQUENCE=AGAAGAGACCTAACTATAATAAAAAGTGAATAAAATCTCATACAAATTTGGATTTCTCTTAACTAAAACAAAAACAAGCTTCTGAATTACTTTTTTCCATTTTTACCCTTTACACAAATCAACCTTATATATTTTCACTCTAGAGAGATAATTTTAGTATATAATAATATATACTAAAATATCAATAAAGTCTTATATATATAACACAAGCAAAACCAAGTAGAGAAAATATATTACAAGAATAACATATATAACAAACTGTTTTTTTTTTGAGGTATAATAAAACAGTTTCAAGTACCATTTGAACATTCAGAAACAGATTCAGTTTCAACTAACTCTCTTTTGTGAAAGTTTCTGTGACAACCACAAGCAGCACAAGTGAGAGCTCCATCTGTTCCTTCTTCACCATTTGCCATGAACTCTCTGCAACCATCAACAGCATAGCCTCCAACATTTGCTGCATGATTCTTCTGACACTCTCCATATTTCACAACACTTCTTTGTGGTTCCTCTGATCTTCTCAGAACCACTTGTCTCTTCCTCATTTCTTTTGTTTTTTTTATACCTTTCCTTTTTTCTTACAAAAAACTTTGGNGAGAGAGCAAGAAGAAAGAGGAAGGGAGGAGGGTAGTTTACTCTGGT
TARGET=194,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05297 Ps_contig_mira-and-tgicl-ass_franssen_37089
SEQUENCE=CGTTTTTTCTAGTTTTGATTAACAAATATGACAATAAATAGGAGATTCATGAATTAAACAATGGCAGTGAAAGAGGAGAAATTCATTTAGTCTTTTACTCATTCATATAAATAAAAGAACCCAAAAAAGGTAACACATAAATTCAAAACACCTAAAATTCGATGTCATTACATTGTCTCAATTGAAATAGCAAAATCTTGTGAGTAAAACTAAAACTATAGCTAGAACCACAAATGTCTAACAAGTAACTCACTTCAGACCTTAGTCAAATAAGCTGAATCCCATGTCATCATCGGATTCTTCCTTCTCTTCCACCTTCTTCTCCTCCTTAGCTTCAGCAGCAGGGGCAGCAGCTGCAGCACCTCCTCCAGAAGCAGCAGAAAGAGCAACGGCACCACCACCACCACCAGATGGAACTGAAGCCAACTTTTCTCTTCCACTGGCAATAAGCTCAGCAAAATCTTTGCCTTTCACTTCACTTAGGAGTAACTCAATATTTGAGTCCTCAGCCTCAACTCCAATGATCCGAGAATCTTCTTAATATCAGCGGCTGAGGGNTGTGTTCGCCCTCCAAGTACCGGACCAAACAAATAGGCGGCGACTTAA
TARGET=393,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0776
PRIMER_LEFT_PENALTY=0.038821
PRIMER_RIGHT_PENALTY=0.038821
PRIMER_LEFT_SEQUENCE=CATCATCGGATTCTTCCTTCTC
PRIMER_RIGHT_SEQUENCE=AGAAGATTCTCGGATCATTGGA
PRIMER_LEFT=287,22
PRIMER_RIGHT=537,22
PRIMER_LEFT_TM=60.039
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.0800
PRIMER_LEFT_1_PENALTY=0.041227
PRIMER_RIGHT_1_PENALTY=0.038821
PRIMER_LEFT_1_SEQUENCE=ATCGGATTCTTCCTTCTCTTCC
PRIMER_RIGHT_1_SEQUENCE=AGAAGATTCTCGGATCATTGGA
PRIMER_LEFT_1=291,22
PRIMER_RIGHT_1=537,22
PRIMER_LEFT_1_TM=60.041
PRIMER_RIGHT_1_TM=60.039
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.1168
PRIMER_LEFT_2_PENALTY=0.038821
PRIMER_RIGHT_2_PENALTY=0.078029
PRIMER_LEFT_2_SEQUENCE=CATCATCGGATTCTTCCTTCTC
PRIMER_RIGHT_2_SEQUENCE=CAGTGGAAGAGAAAAGTTGGCT
PRIMER_LEFT_2=287,22
PRIMER_RIGHT_2=441,22
PRIMER_LEFT_2_TM=60.039
PRIMER_RIGHT_2_TM=59.922
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.1168
PRIMER_LEFT_3_PENALTY=0.038821
PRIMER_RIGHT_3_PENALTY=0.078029
PRIMER_LEFT_3_SEQUENCE=TCATCATCGGATTCTTCCTTCT
PRIMER_RIGHT_3_SEQUENCE=CAGTGGAAGAGAAAAGTTGGCT
PRIMER_LEFT_3=286,22
PRIMER_RIGHT_3=441,22
PRIMER_LEFT_3_TM=60.039
PRIMER_RIGHT_3_TM=59.922
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=156
PRIMER_PAIR_PENALTY_4=0.1487
PRIMER_LEFT_4_PENALTY=0.038821
PRIMER_RIGHT_4_PENALTY=0.109882
PRIMER_LEFT_4_SEQUENCE=CATCATCGGATTCTTCCTTCTC
PRIMER_RIGHT_4_SEQUENCE=AGATTTTGCTGAGCTTATTGCC
PRIMER_LEFT_4=287,22
PRIMER_RIGHT_4=462,22
PRIMER_LEFT_4_TM=60.039
PRIMER_RIGHT_4_TM=59.890
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=176
=
PRIMER_SEQUENCE_ID=SSR_05298 Ps_contig_mira-and-tgicl-ass_franssen_37091
SEQUENCE=ACACCAGCTANNTCATATCATATCATATTGTGATGATAACTTTGGTTTCTTATTATTCAAATTTTCATATACATGAACTAAGCTTATCTTAAAGTAATAATAATTCTTATGGAAGTTATTCTGCAGCCTCAAGCCTTCAATGTTAGAGTTCAGTTTCTCCACACTACAAAAAGTTATCATGTACAGAATCCAGTAATGCATATAACATCATCACCATGCCAAATTCAATGTTTTAGTTCTTCCATTCATCAGAGACTGACTCAAGTTGCAGATGATTGCAGAAGTAAAACAAAGGTTTTTGCGATGAGAAGGAGAAGGTCGAATGAAAGGACAGAAACTTATGTGCTATTAGAACCGGGGAAGGATGAGAGGTTTGTGTCGGAGGAAGAGCTGAAAGAGATTTTGAAAGAGTGGCTTACGAATTGGCCGGGCAATGCTCTTCCTCCTGATCTTGCAAGATATGAAACCATTGATGATGCTGTTTCTTTCTTGGCAAAGTCGTTTTGTGAACTTGAAATTGATGGAGAAGTTGGGTCGATTCAATGGTTTGAGGTTCGGTTGGAGTGAATAGAATTCAGATTATTCCATCAAACCGTATCAACTGCGGTTTGCCAACATGTGCTTGTAATGTTTAGTTTTGTTCCTTTCTATTGTAACTGACACAAACATGTTATTTAGGCCCGGTTTTGATAAACAGTCGAATTAAGTGCTTATAGCRTAAATAC
TARGET=13,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05299 Ps_contig_mira-and-tgicl-ass_franssen_37094
SEQUENCE=AATAACAAGAATAATAGCTCTACAGATAGTATTCTTTCGAGCGACGAAAATGCATTTCTTGAAACATCAAATAAAAAAGAAGATGTGAACTTGCCACACATGGCGAATGTTCATATGCCGATGCATATGCCGTGGCCTTACAATGTACCGCCCTATATGTATAATCTTCAAAATCCCGGTCAGCAAATGCCGTCATACCAAGGATATCCTCCTTACCTTCAAAATAATATGCATTGGCCATCGAACATGGGAGTGAACCAAAAACCGTCAGCGACTAAAAGAGAGAAGTCTCGTTATAAGAAAGGATCGGAAGAATATGAAGAACCGGAAACAGATTCCAGTGATCCTGATTCTGGGAGTGAATCTGATTCGGATAAACAGAAAGACTCGAATAATTCGTTGAAGGATGATAAGAGGAAAAAGCACAGAAGAAAATCGTCCGGAACGGTTGTGATACGTAATATCAACTACATTACTCCGAAAAGGAGAAATGGAAACGAGAGTGGAGGTTCTGATGATTCTTCTTTAGAAGACGATGTTGTCTTCGACGAAGAAACCATCAAACAGAAGGTTGGTGTTGCACTTGAATCATTGCAGAAGGTTCATAAAGGTGAAAAACGTGGTAGTCGAAAAAAGTCATCGGCTAAACACAATGTAACTAAATCGAGTGATGATGCGGAGGAAGATGCATCTGAAGGTGGGAACAAAAAATGAGAACTGGAATGCCTTTCAGAGCCTTTTGAAGATAGATTCCGATACCGAAGTTGACGGATCAGAACAGAAGCAATCAATCGATGTCCAGGATGAACATTTTGTGTTGAGCAACTCTGAAGGAAGAATGTCGTCTGCTGCTAGTTCTGCTCCAAACATGGATTTCAATGAAGTTCCGAAGAATCGTGAAGTTGCGAATGACTCCTTCATTGTGACTCAGAGAGACGGAGGAAATGGAGGCGAGTCGAAGAAGGGCGGATATGTAGAAAATTTCGGCCCAATTACAAAGATCAAAGAAAATATCGGTGAAGATATATTGTTATCACACATATCAAGAGAACCGAGAAATGAAATTGGCGATCCATTAAATACTTTTGCTGCCGATTCGTCATTACAAACCAAGGGAAGAGGATCGGATGATTGGTTTATAGTTGATAACAATTTGGAAAGTACGAGAGGCCGTGATCCCTCAGTTATTCCAATAGTGTTCGATTCTTCCCACGCTGAAAAAAGAAGTGAAAAAAATATCATCGATGATTCTTTCATGATACACGGTGATCAGTTGGTAGATAACAATCTTTCTGATTCGCAATGGAAGACGGATATGAGCATGGTCGAAAATCTAACATCTTCCAACAAAGTAGAAAGTGAGACAAAAGAAAAGAACACACTTTCAAAGATAGAGGAGCCAAATGATCTCTGTGTAGTTCTACGGCGCGATTCAGGATTGGATTCTGTCGAGGCCTCACGAACCATGGACTACGAAATTGACTTTTCTTACTCAGAACCTGATAGAAGAACTTCTATCGATGATTCGAGTGTTAACGTGAATAACAACAATCTTCCAAGTAGTCCCAAGAAAACCAACGCGATTAAAACCAAAGTCTCGCGATTACCTGCCAAGGGAAAGCCCGAGATAATTCCTAGAAGCAGAAAACCTTCTGTCCCAAGTAGGCCTATAGTTCAAAAGAGCCAAAGAGAAAAAGGAAGATGAAATTAGGAAGAAGTTGGAAGAAAAGGCGAGTGAACGACAGAGGAGAATTGCCGAAAGAACTGCATCTTCTGGTGTTGGTCGAGGCGTGCCCGGGAAACCGATAAGAACAAGACTCAAGCTGTTAAAGAAACAAAGAGAATCAGTTCTGTTAAGGTCAGGGGAGTTTAAGAAAAATCTGAATGCATATCTTATAAAGTATATAGGTGATTAAGTGTATTTAAATGATTGATGATTGTGATTAAATTAAAATTGAAATTATTTGTTTTGTTTTGTATAAATAAACAAACTCTTAAGGTCATTGGAAATTTTCAGACCTTTATAGTTACTCAAAATTGAGATTTCTTTTTGAATTTTTGATTCATGGTTGTGTATTTAGTATTCNTTATTCTCTGTAATGTATGAAACATTTGTATGTGCAAACAACAATGAAAATTTGGTCTTTTTTGTTTTGAATTAAAAACT
TARGET=1964,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05300 Ps_contig_mira-and-tgicl-ass_franssen_37098
SEQUENCE=GAGNGAAAAGAAAACGGGAAAGCGGGGAAGACCGGTTGGGATTGGACGATGGTTAGAAGCAAATTCTCGCACTTTCCCCAATTTCCATCTTTCATCTTTTTCCGTTACCTAACAAACCATTCCTCCACCACCACCATCACCACCGATTCATCAACTCCAAAACGTTTCTTTCTTAATCTTCCTAACTAGGTGTTTTGGATTTTGGTTCGAAGTTTCAGGAATCAAGGAGTAAATAAATCATTTGTGATATTTTAAGGACATACATTCTACACAAAATATCATGGAGGAAACAATGGCAGCAAGATATTGGTGTCACGCTTGTTCTCAAATAGTCAGTCCTATCATGGGAATGGAGATGAAATGTCCAGTTTGCCAAAGTGGATTTATTGAAGAAATGAGCAGTGCAACTGGTATCAATGACTTGCAAAATACACCAGAAAATGATTTTGGATCGGATCGTTCTCCCTCCCTTTGGGCACCAATCTTGCTAGGCATGATGGGCAATCCGCGGCTTCGTCGGAGATTGAGACGGATAGAGCTTGAAGAGGATTATGATAGTGGGAATCGCGATGGTGATAATGGTAATGGTGGTGTAGGACATTATGATCGAGAGCTCGAGTCCATCCTTCGAAGGAGGAGAAGAAGCTCTGCTACTATTCAGCAATTGCTCCAAGGCATAAGAGCTGGACTGACATCTGAGTCGCATGAGAATACGGATAGGGATGACCGTGTTAGGGAAAGAGAAAGAGAGCATGTAATATTGATTAATCCTTTTAATCAGACTATTATTGTTCAAGGCTCCTATGATTCAAACCGGGACCAAAGTGATAATCATAACCCCATTGGGTCTTTGGGTGACTATTTTGTAGGGCCTGGTTTGGATCTATTGTTGCAACATTTGTCTGAGAATGACCCTAATAGGTATGGAACTCCACCTGCTCCAAAGGAAGCCGTTGAAGCTTTACCAACTGTGAAAATCAATGAGAATATGCAGTGTTCTGTCTGTTTGGATGATTTTGAAGTTGGTTCCGATGCCAAAGAGATGCCATGTAAACATAGGTTTCATAGTGCTTGTATCCTGCCCTGGCTGGAGCTTCATAGTTCTTGTCCGGTTTGCAGATCTCAATTGCGAGTGGATGAACCCAAGCAAAACTCTGATGTGTCAAGGAATAATAGAAATCAAAGGGAGAATGAAAATATTGGACATGCCAATAATGCTGAAAGGGATAGTGAAGGGAGAAGTGCAAGTGGGGGTAGAAGATTCTCATTTCCATGGCCTTTCAATGGATTATTTTCATCTTCATCATCATCTAGTTCAAATGCCAATGGAAACAGTACTCACCCAGAAAGAAACTGAGTATTCACGCAGATCCGTTGTATATTTCATGTGCTGCATTGCTTTTGTACAGAGAAAACTACCGATTAAAAGATACAACGTTGACATTAAGTCGGTTTTGATTTTTTACACCACTTTGTTTTGCTAAATTTCGTGCACCATTG
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0475
PRIMER_LEFT_PENALTY=0.027715
PRIMER_RIGHT_PENALTY=0.019805
PRIMER_LEFT_SEQUENCE=AGAAGCAAATTCTCGCACTTTC
PRIMER_RIGHT_SEQUENCE=CCTGAAACTTCGAACCAAAATC
PRIMER_LEFT=54,22
PRIMER_RIGHT=218,22
PRIMER_LEFT_TM=60.028
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=0.0475
PRIMER_LEFT_1_PENALTY=0.027715
PRIMER_RIGHT_1_PENALTY=0.019805
PRIMER_LEFT_1_SEQUENCE=AGAAGCAAATTCTCGCACTTTC
PRIMER_RIGHT_1_SEQUENCE=CTGAAACTTCGAACCAAAATCC
PRIMER_LEFT_1=54,22
PRIMER_RIGHT_1=217,22
PRIMER_LEFT_1_TM=60.028
PRIMER_RIGHT_1_TM=59.980
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.2034
PRIMER_LEFT_2_PENALTY=0.027715
PRIMER_RIGHT_2_PENALTY=0.175676
PRIMER_LEFT_2_SEQUENCE=AGAAGCAAATTCTCGCACTTTC
PRIMER_RIGHT_2_SEQUENCE=GAGAACAAGCGTGACACCAATA
PRIMER_LEFT_2=54,22
PRIMER_RIGHT_2=325,22
PRIMER_LEFT_2_TM=60.028
PRIMER_RIGHT_2_TM=60.176
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.2562
PRIMER_LEFT_3_PENALTY=0.027715
PRIMER_RIGHT_3_PENALTY=0.228464
PRIMER_LEFT_3_SEQUENCE=AGAAGCAAATTCTCGCACTTTC
PRIMER_RIGHT_3_SEQUENCE=GTTTTGGAGTTGATGAATCGGT
PRIMER_LEFT_3=54,22
PRIMER_RIGHT_3=162,22
PRIMER_LEFT_3_TM=60.028
PRIMER_RIGHT_3_TM=60.228
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=0.2567
PRIMER_LEFT_4_PENALTY=0.236882
PRIMER_RIGHT_4_PENALTY=0.019805
PRIMER_LEFT_4_SEQUENCE=ATGGTTAGAAGCAAATTCTCGC
PRIMER_RIGHT_4_SEQUENCE=CTGAAACTTCGAACCAAAATCC
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=59.763
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_05301 Ps_contig_mira-and-tgicl-ass_franssen_37101
SEQUENCE=ATCAACGCRGARTANNNNGGGGCATTCAAACCATAAAAAAAGTAGCATGATTGCAGCATCATAGACGAACGTCACTCTCAGTCGAGAATCGGAGAAACGAGAGTTCTGAACAGAACCCTAACAACATCACTCAATCTCTCTCTCTATGGCATCCAATTCCAACGCCACCAACACGCTACTTTCCATTTCCTACAACCACGGTTCTCTTCAGCTTCTCGATCAGAGGAAGCTTCCTTTAGAAAGCGTTTACTTGGAAATTCGGGATTCAAACGATGGCTGGAATGCTATACGAGATATGGTGGTCCGAGGAGCACCTGCTATTGCTATTGCTGCAGCTCTCTCTCTGGCTGTAGAGGTATCCAATTTAGATGATTTCAATGGATCTACTGATGATGCTGTATACTTCCTGCAGAAGAAGTTGGACTATCTTGTCTCGAGTCGGCCAACCGCGGTGAACTTATCAGATGCTGCGACAAAACTCAAAGAAATCATATTGAAAGCTGCTGCTACAACTTCAGAGGCTAGGGGTGTTTTTCAGGCTTATGTAGAAGCTTCCGAAATAATGCTTGAGGACGATGTTGCATCAAACAAAGCAATTGGGACTTATGGAGCTAAATTTATTCAAAACCATACAGAGAAACAAAAGCTTTCTGTTTTGACCCATTGCAACACTGGAAGTTTAGCCACAGCTGGATATGGTACTGCTCTTGGTGTAATCCGTGCCCTTAACAGTGGAGGAGTTTTAGAAAGGGCTTATTGTACTGAAACACGCCCATTCAATCAAGGATCCAGACTTACTGCCTATGAGTTGGTACATGAAAAAATACCAGCAACTCTCATAGCAGATTCTGCTGCAGCTGCACTAATGCAAGCAGGCCGTGTAGATGCTGTTGTTGTTGGCGCAGATCGTGTTGCATCAAACGGTGACACGGCCAACAAAATTGGAACCTATAGTGTTGCCTTATGTGCCAAGTTTCATAATGTACCTTTTTATGTTGCTGCACCCTTGACTTCCATTGATCTATCACTTTCTTCTGGGCAACAAATTGTCATTGAGGAGAGATCTCCCAAGGAGTTGTTGAACTCACGCGGAGGACTCGGAGAGCAAGTTGCTGCCTCAGGAATATCTGTGTGGAATCCGGCGTTTGATGTTACACCTGCTAATCTAATTTCTGGGATCATCACTGAGAAGGGTGTCATTACAAAGACTACTGCTGGTGATGCTTTTGACATAAACGCTTTCGTACAGAAAACAGGTTAAGGGTGGAATCTATTCAATATAAATGATCTATGGTGTGAAATGAAATGTGTTTAATCAAATTGTAGATTTGAGTTGTATGTTTTAGAGGGGAGTTGTAGATCAACTCTTTCAATGTAAGTGTTCCGGAGAAGAAACAGGGAGCTGGCTAATAAACAAGTCAAAAAGAAATTGGTGGCGACCATGACTTGCGCAGCTAAAATCGAGTGGTGTAATTTATCGGTTAATGCAAGATATGGTTTTATCAGTTTTGTTTTTGACAACACAAGACATGTTTGTATCAGATATAATAAGGTTCTAAACTATGTAATGCTGCATTTGACATCAAATAAGATTATTATACTGTATGGTTCTTATACNTTCGACTACTTTGAGAA
TARGET=135,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05302 Ps_contig_mira-and-tgicl-ass_franssen_37101
SEQUENCE=ATCAACGCRGARTANNNNGGGGCATTCAAACCATAAAAAAAGTAGCATGATTGCAGCATCATAGACGAACGTCACTCTCAGTCGAGAATCGGAGAAACGAGAGTTCTGAACAGAACCCTAACAACATCACTCAATCTCTCTCTCTATGGCATCCAATTCCAACGCCACCAACACGCTACTTTCCATTTCCTACAACCACGGTTCTCTTCAGCTTCTCGATCAGAGGAAGCTTCCTTTAGAAAGCGTTTACTTGGAAATTCGGGATTCAAACGATGGCTGGAATGCTATACGAGATATGGTGGTCCGAGGAGCACCTGCTATTGCTATTGCTGCAGCTCTCTCTCTGGCTGTAGAGGTATCCAATTTAGATGATTTCAATGGATCTACTGATGATGCTGTATACTTCCTGCAGAAGAAGTTGGACTATCTTGTCTCGAGTCGGCCAACCGCGGTGAACTTATCAGATGCTGCGACAAAACTCAAAGAAATCATATTGAAAGCTGCTGCTACAACTTCAGAGGCTAGGGGTGTTTTTCAGGCTTATGTAGAAGCTTCCGAAATAATGCTTGAGGACGATGTTGCATCAAACAAAGCAATTGGGACTTATGGAGCTAAATTTATTCAAAACCATACAGAGAAACAAAAGCTTTCTGTTTTGACCCATTGCAACACTGGAAGTTTAGCCACAGCTGGATATGGTACTGCTCTTGGTGTAATCCGTGCCCTTAACAGTGGAGGAGTTTTAGAAAGGGCTTATTGTACTGAAACACGCCCATTCAATCAAGGATCCAGACTTACTGCCTATGAGTTGGTACATGAAAAAATACCAGCAACTCTCATAGCAGATTCTGCTGCAGCTGCACTAATGCAAGCAGGCCGTGTAGATGCTGTTGTTGTTGGCGCAGATCGTGTTGCATCAAACGGTGACACGGCCAACAAAATTGGAACCTATAGTGTTGCCTTATGTGCCAAGTTTCATAATGTACCTTTTTATGTTGCTGCACCCTTGACTTCCATTGATCTATCACTTTCTTCTGGGCAACAAATTGTCATTGAGGAGAGATCTCCCAAGGAGTTGTTGAACTCACGCGGAGGACTCGGAGAGCAAGTTGCTGCCTCAGGAATATCTGTGTGGAATCCGGCGTTTGATGTTACACCTGCTAATCTAATTTCTGGGATCATCACTGAGAAGGGTGTCATTACAAAGACTACTGCTGGTGATGCTTTTGACATAAACGCTTTCGTACAGAAAACAGGTTAAGGGTGGAATCTATTCAATATAAATGATCTATGGTGTGAAATGAAATGTGTTTAATCAAATTGTAGATTTGAGTTGTATGTTTTAGAGGGGAGTTGTAGATCAACTCTTTCAATGTAAGTGTTCCGGAGAAGAAACAGGGAGCTGGCTAATAAACAAGTCAAAAAGAAATTGGTGGCGACCATGACTTGCGCAGCTAAAATCGAGTGGTGTAATTTATCGGTTAATGCAAGATATGGTTTTATCAGTTTTGTTTTTGACAACACAAGACATGTTTGTATCAGATATAATAAGGTTCTAAACTATGTAATGCTGCATTTGACATCAAATAAGATTATTATACTGTATGGTTCTTATACNTTCGACTACTTTGAGAA
TARGET=336,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05303 Ps_contig_mira-and-tgicl-ass_franssen_37109
SEQUENCE=CGGGGACAGATTCATTTCTCTCAACGCTTTCTCTTGTTCATCTTCCACTTTCAACAATTCTTCAATAACCCATCAAAAAGTGTGTAGATATATTTTCTGAAGAAGAAAATGATGAGTAATGAAGAGTTAGTTATTCAAGACGAGTATCTTTACAAAGAAGAGACGCGACTCACTGTTCTGAAAACGTCTCGGTTCTTCACTGGTGATGGATTTGTCGTGTACGATTGTAAGGGACAACTCGTTTTCCGATTCGACTCGTATGGACCTCACACTCGTGATAAGGAAGAACTTGTTCTCATGAATCCTCATGGTCGTTCTCTTCTCACCTTGCGTCGAAAGAAACCTAGCCTTCATCAACGATGGGAAGGGTTCAAAGGAGAGAGAAAGGACGGTGATAAACCTATTTTTAATGTAAAAAGATCGTCAATAATCGGACGGTCACGAACAACCATAACTGTGGAAGTACACGATAATTCCGGTGTGGAGTACTTCATTGAAGGGTGCTTCCCACAACGATGTTGTAAGATTTTCAACGCCACAAAAAAATTAGTGGCTGAGATTAGTCGTAAAGTGGACCCCACCACTAATGTTATGCTTGGAAAAGAAGTGTTCATGCTTTGCATTCAACCTGATTTTGATGTGTCCTTTGCCATGGGATTGGTTTTGGTACTTGATCAAATCAACGGTGAGAATTTCTTTGATAATGGGATGGTCGAGACTTCTGTGCACCCTACTGCAGAAGATTGAGGAATTGGTTGTTCTTCTGTTTTATTTTAAGATTTTTATTTTAAACCAAAAATATTACTCACTTAAAAGTTTAATGACCTTCTCAAGACATTTTTAATTTTTTTTGTTTTCTTCTTTAATATTTATCGGTCCATTATTGTATGAGATTAATAAAATGGTTAATGGTGGATATTTATATCTTTCAAAATTCAATACATGCTTCAAATAAATAAATTTTGTATATCAATCTACG
TARGET=950,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05304 Ps_contig_mira-and-tgicl-ass_franssen_37110
SEQUENCE=AAAAGAACAAAAGGTAACTAACTGATTAGGGTTTTTTTTGCATCTAGATTTTCCACATTCTATTCTCTGTTCACACTTCACTCTCACAACAACAACAACAACCAGATCCATATTTGAGTCAGCAAAAGCAACTATGTTGTTGTATTAGTTAAGACAACAGTGTGTGTCTGGAAAGAGTCTTGGAATTAGCTGAGACGAATAAGGAACGAGAGGATGTCAAGTAGTAGGGATACTCATTGGTGCTATAATTGCATGAAATCGGTTAGATTGGGACGGAGACATGCAGTTTGCTCTGGTTGTGATGGGGGATTTGTTCAAGATCTTGATGATATGGTTCATGGTAGCCCTCCAGACTTTCATGGAGTGGACAGCGATGRNCAAATTCCATCCAGGTTTTCTAGACAAGGTGGCATTGAAGCTCTCTTAAACGGAACTCCCGGAGTTGGTGTGACTAGAGATAACTCAGGCGACTATTTTATCGGCCCTGGAGTTACAGAACTGTTTGAACAGCTTTCGGCTAATGACCAGCGAGGTCCCCCGCCTGCATCGAGATCCTCTATAGCTGCAATCCCTACTGTACAAGATTGCGTCGAAGCATCTTCA
TARGET=86,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05305 Ps_contig_mira-and-tgicl-ass_franssen_37117
SEQUENCE=AATTAGAAGCTAAGATTTGATAGAAGGGGAAAAAAGACAAGTACTACGGTATATTTTTATACTAAAGGACTGCGATGGAATAAGACAACATGTACTAGTAAACCTCCAAATGGAAAATATACCATACATATCTCAGAACAGTTCCTTTCTAATTACTTGTCACTCTCACATATTATATATTCCTAAAGCAGTAACTAGTAATATGACAAATGCTACAAAGATGAATTATGAGGTTGGAAATTAAGCTCTCATTAGGGTGATGCTGGGATGGGTTCAGTACCAGAAACATGGGACGCAGCATCTTCTTTTGACATAGCCACATAATTCACAGGAGCAACAGGTCTCGTATTATCTCTTTTTCCATCTTTTGCTATGGACCTGCTTCCATTTTGATGAGCATTAAGCGATAGCCGTCGGCTAGGAGTACCGTTGGCTCCTCCATTAACACGAGGGCCACCTCCCTTCTTAGCGCTAACTGGCCTAGCAGGACTGGGTTTTGAACCAAATGGGGCTTCTGGTTCTGCGATTTGCAGCTCCTGAAATTTCTTCTGGTCCCTCATTCTTCGTTTTTCTTCTTCCCGTTCATGCCTAAGCATGGCATATTCATCTAACATGGCTAGAAGGGGAACACCATCATATGTGAATGACATATCATGAGTCTCTTCCCATGCACGTGTTTTAGCAACCAATGTCTCAACCAAAGCAGGAATTTTATTTACCAATATCCGAGCTTTCTCTGCTCGTTTGAGGTTTAAGTGTGCACCTCTGCTTGCATTATACCTGTTCTCGTCCCGATTATAGTCCTCAAGCCAGCTCTCTTCTTCACATGCTGACATCCATTTCTCAACCTTGTCCAATATATCTTTTCGGCTTAGAGCTTCTTCTTTCGCTTTTGCTATTTGATTGTCCATGTCAGCCAGTAATTCAGTTGGTTCAATGTCTCCAGAATCAATCAATGCCAAGATCTTCTCTCTTGCAACATCTGAGTCTATTTCTATATGAGCATGGGCAAATATCTCTTCGAGTTCTGATTGTTTCTTGAAAGCAATTTCCTTCATCCTGCTTGCTTTAAGCTGGTCGAGTCTCTCAACTTCCACTTCAGCCTGCTCAATCAGATCCAGGGCAAGGGAACCAGGAATAGTAACTTCATCAACGGAAGCTGAGATGTTACAGGTAACATGGTCAAACAGTTTCCTTTCCTCTTGAGGAGTATCCATTAGATTCCAAAGATCAATTAACTGAGAAGCTAATTCTTGGAGCTTGTGTAGCCTCTGCTTTTTATCTTCTTTCAGCGTTAAGACTGTGTTAGCGAGCCTAGCTAGAGTGTCATTGCTTATACTTTTGGATTGAACGCCAGTCGAATCATTCAGGCTTGGATGAACCTCAGTTACAGTACTGAAGAAGTCCATACCAAGGACAGCACAAAGATCATGCACAGTACTCACCAATTCAAAAACCTTTTGCAATCTATCACTCTTTTCCTTTTGAAGTTCATGAAGCTCAGATTGATATTCCTCAAACTTCTTCAAGGACAGGTCAGACTCATCAACTGCAGGTACATCATTATGGTTCAGGCTCCCGGTTATCTCTCCGCATATTCTTTGGATCTGTGATTGTACATCTGAGAAATCCTTTATTCTTTCTTCCTTTTGTTCCCATAACTGTTCCAGCACTGGTGCTATAGCTGCAAGCTGTTCCTTGATAGTTCCAGAAGTATTATCGGGAATTCCAGCAAAGCTCTTTTCTCCAAGAGCTGATAGAAGAGTAGAAAGCTCAAGCTTAGCATCAGACAAGGATTGAAGTAGCTGTGCCCTCGACTTTGTAGCATGCTCAACCTTTCTCTTGTAAACATCCAAGCACTCCTGCTCTAACTGAAGAAGCATCTTGTCCCGTTCTTCATCACTTTCACCAACTTCATCCCATATTTCCTGCAGCTTCTTCAACAAGGAACCACAGGTGTTTTCTCCAATTAGAGGGTTTTGAGCTTCAGTTACCGCCATTGAGTTCCAGAGCAACCTAGCCGTATGAGGCTGGGTTAGGGTTAAGAGATTTTCAGATCTGAAACAGTGTGAGTGAAAAAAGAGTGAGTAGTGTGTAGTTATAATAATGGTGGTAGTAGTAGTATTAGTAAGTGATGAGTGATCCCCNATTAGATTTTAGATT
TARGET=2113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1717
PRIMER_LEFT_PENALTY=0.146525
PRIMER_RIGHT_PENALTY=0.025128
PRIMER_LEFT_SEQUENCE=CGTTCTTCATCACTTTCACCAA
PRIMER_RIGHT_SEQUENCE=AATNGGGGATCACTCATCACTT
PRIMER_LEFT=1890,22
PRIMER_RIGHT=2150,22
PRIMER_LEFT_TM=60.147
PRIMER_RIGHT_TM=59.975
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.50
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.50
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.75
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.2304
PRIMER_LEFT_1_PENALTY=0.205226
PRIMER_RIGHT_1_PENALTY=0.025128
PRIMER_LEFT_1_SEQUENCE=AGCTTCTTCAACAAGGAACCAC
PRIMER_RIGHT_1_SEQUENCE=AATNGGGGATCACTCATCACTT
PRIMER_LEFT_1=1932,22
PRIMER_RIGHT_1=2150,22
PRIMER_LEFT_1_TM=59.795
PRIMER_RIGHT_1_TM=59.975
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=5.50
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.50
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=0.2491
PRIMER_LEFT_2_PENALTY=0.224014
PRIMER_RIGHT_2_PENALTY=0.025128
PRIMER_LEFT_2_SEQUENCE=CCATATTTCCTGCAGCTTCTTC
PRIMER_RIGHT_2_SEQUENCE=AATNGGGGATCACTCATCACTT
PRIMER_LEFT_2=1919,22
PRIMER_RIGHT_2=2150,22
PRIMER_LEFT_2_TM=60.224
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.50
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.50
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.75
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.2521
PRIMER_LEFT_3_PENALTY=0.226971
PRIMER_RIGHT_3_PENALTY=0.025128
PRIMER_LEFT_3_SEQUENCE=AACCACAGGTGTTTTCTCCAAT
PRIMER_RIGHT_3_SEQUENCE=AATNGGGGATCACTCATCACTT
PRIMER_LEFT_3=1948,22
PRIMER_RIGHT_3=2150,22
PRIMER_LEFT_3_TM=59.773
PRIMER_RIGHT_3_TM=59.975
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.50
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.50
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.75
PRIMER_PAIR_3_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.2682
PRIMER_LEFT_4_PENALTY=0.243031
PRIMER_RIGHT_4_PENALTY=0.025128
PRIMER_LEFT_4_SEQUENCE=AACTGAAGAAGCATCTTGTCCC
PRIMER_RIGHT_4_SEQUENCE=AATNGGGGATCACTCATCACTT
PRIMER_LEFT_4=1869,22
PRIMER_RIGHT_4=2150,22
PRIMER_LEFT_4_TM=59.757
PRIMER_RIGHT_4_TM=59.975
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.50
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.50
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_05306 Ps_contig_mira-and-tgicl-ass_franssen_37118
SEQUENCE=TCGGGGAAAACGCCTAATCCCTAAAAATTGTTTGGAGAACTCACTACACAAAACCAACAATGGCGTCACCGGCAGCAACCATGGGTATCGCCATGACCGTAACTTCACACTTCTCAACCTCTTCTCGTCTTCTCGCTTTCCAACCAAACCTTATACCACACAAACGCCAAACCAAAACCTTTTCAATTTCAACCAATCGTAAAACCACCGTAACCGCCTGCACCGTGACTACCGCACCACCGGCACCGCAATCTCCTGTCGACGACGACACCGTTAATCTCTCTGGTTCTACTCGAACTGTGACCACTCTATTCGGAATTGCGGTTTTGTGTATCAAAGCTTTTGTTAACATTCTTCCACCGCCGGAACTATGTTTATCGATTGGAACTTCGTCGTCGTCGCTGTTCTTTGCTGCTTTTGTGAATAAGAGTACTCGGGGTTCGCTGAGCACGCCGTTGACGGTTGTAGCGGCGGGATTGAAGAAATGGTTAGATATTTACAGTGGTGTTTTGTTGGTTAGGGTTTTGTTGAGTTGGTTCCCAAATATTCCGTGGGAAAGACAGCCACTTTCTGCAATTAGGGATCTTTGTGATCCTTATCTTAGTTTGTTCAGGAACGTTATTCCCCCTGTTTTTGATACTCTTGATATTAGTCCTCTTCTTGCTTTTGCTGTTTTGGGAACTCTCGCTAGTCTCCTCACTGTTCCTGTTTAGAACTACCCTCACAGGTGTGGGCTACTCGAGAATGTTTTCATGACCGGCAAAGCATCGCCTTCCATAGTTTTGGCAGAATTTTGAAAAACAGGTTCTTGAGAAGCATAGGATTAAGTTTGGTTTCTCTATACAATGTAATTTTTCAGATTGATATATTTTGGTAGTTATGCATGTGGTTATGAAAGTCGATGGATTTTCATTTTCGAATAATGTATGTTGTGTGTTATTATTGGGTTGGATAGTTTAGTTGTGTATCTTAATTGAAATTCATTGACTATGAGCTCTGCCCAGAGTTTGCAAGCTAGTAATCTTGCTCTGGCATAAATATAAAATACATGTTCGATATTGGTTATGAAAATATTATGCACGGTGAATGTTTTGACATCT
TARGET=390,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2573
PRIMER_LEFT_PENALTY=0.203324
PRIMER_RIGHT_PENALTY=0.054021
PRIMER_LEFT_SEQUENCE=CGAACTGTGACCACTCTATTCG
PRIMER_RIGHT_SEQUENCE=ACCCTAACCAACAAAACACCAC
PRIMER_LEFT=293,22
PRIMER_RIGHT=522,22
PRIMER_LEFT_TM=59.797
PRIMER_RIGHT_TM=60.054
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.6000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.2578
PRIMER_LEFT_1_PENALTY=0.203324
PRIMER_RIGHT_1_PENALTY=0.054514
PRIMER_LEFT_1_SEQUENCE=CGAACTGTGACCACTCTATTCG
PRIMER_RIGHT_1_SEQUENCE=GGAATATTTGGGAACCAACTCA
PRIMER_LEFT_1=293,22
PRIMER_RIGHT_1=549,22
PRIMER_LEFT_1_TM=59.797
PRIMER_RIGHT_1_TM=60.055
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.3812
PRIMER_LEFT_2_PENALTY=0.203324
PRIMER_RIGHT_2_PENALTY=0.177853
PRIMER_LEFT_2_SEQUENCE=CGAACTGTGACCACTCTATTCG
PRIMER_RIGHT_2_SEQUENCE=CCCTAACCAACAAAACACCACT
PRIMER_LEFT_2=293,22
PRIMER_RIGHT_2=521,22
PRIMER_LEFT_2_TM=59.797
PRIMER_RIGHT_2_TM=60.178
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=0.4256
PRIMER_LEFT_3_PENALTY=0.203324
PRIMER_RIGHT_3_PENALTY=0.222249
PRIMER_LEFT_3_SEQUENCE=CGAACTGTGACCACTCTATTCG
PRIMER_RIGHT_3_SEQUENCE=CCAACTCAACAAAACCCTAACC
PRIMER_LEFT_3=293,22
PRIMER_RIGHT_3=535,22
PRIMER_LEFT_3_TM=59.797
PRIMER_RIGHT_3_TM=59.778
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.6000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.5059
PRIMER_LEFT_4_PENALTY=0.203324
PRIMER_RIGHT_4_PENALTY=0.302618
PRIMER_LEFT_4_SEQUENCE=CGAACTGTGACCACTCTATTCG
PRIMER_RIGHT_4_SEQUENCE=ATTGCAGAAAGTGGCTGTCTTT
PRIMER_LEFT_4=293,22
PRIMER_RIGHT_4=576,22
PRIMER_LEFT_4_TM=59.797
PRIMER_RIGHT_4_TM=60.303
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_05307 Ps_contig_mira-and-tgicl-ass_franssen_37123
SEQUENCE=TGGTATCAACGCANAGTGCNCGGGGAAAAAGAAAACTAGAAAACAAAACTTCTTCTTCTTCTTTCCCACAACCTCTACCGTGCCTCTCTCCTCTCTCTGACCACTTGGCTCATTTTCAAGGAAGGGAGAGAGAAAAGCTGTGCAGCTTTTCCTCTTCATCACCGTCCAAAACAACCCAACCTTCATCCCTCACACACACTGCACTAACCATTCTCACCCAACCCCACCGTTACTGCTCCTCAACCTTCATCGGACCTCCGCCACAAACCACAACACAACCACATGTGATAATCTTCTTCCAAGCCCTCCGCCGACACAATTCTCGTTGCTATTCATCGCCTTCACAGCCGCGCAAACACCTTCGCACCACCGGCAAAACCCACGTCAGATCCGGCCACGATACCGAAATCTTATGTATGCACCTCCATCCTGCCGAAAGTTGAATAAATGGTGGGATGCCTTAAAACAACGACAACATCCGCAACAACGGTAACGATTTGGAGTCGTCGCGGTTAAAACGTATGCTTGCTGAAATCTAGATTTGTGGTGCGATTTATTGTGAGTGTGGTGCTCTAGTTTCAGATTATTGAAATTCTTTCAATTTGCGCCATCGATTCATTTCGGTTCCATGCCTCGTAACATTTCCAGGCTAGGTAAATTTTATTGAATGGTTGACTTAAATTTACTATATTCATGTGTGTTCAAATGTATAACTCAAGGTTTTGTGTATCACTCTCCATTACTATTGATCGTATAAATTGGATGAGAATGTGTTAATCCTGAGTTTGCATAGGAAATATTTTCTTATTATTAAATCCTATGTTCATTTGAA
TARGET=49,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2229
PRIMER_LEFT_PENALTY=1.160537
PRIMER_RIGHT_PENALTY=0.062402
PRIMER_LEFT_SEQUENCE=CNCGGGGAAAAAGAAAACTAGA
PRIMER_RIGHT_SEQUENCE=GGAGGGCTTGGAAGAAGATTAT
PRIMER_LEFT=18,22
PRIMER_RIGHT=308,22
PRIMER_LEFT_TM=61.161
PRIMER_RIGHT_TM=59.938
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=1.2564
PRIMER_LEFT_1_PENALTY=1.160537
PRIMER_RIGHT_1_PENALTY=0.095831
PRIMER_LEFT_1_SEQUENCE=CNCGGGGAAAAAGAAAACTAGA
PRIMER_RIGHT_1_SEQUENCE=ATGTGGTTGTGTTGTGGTTTGT
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=283,22
PRIMER_LEFT_1_TM=61.161
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=1.2999
PRIMER_LEFT_2_PENALTY=1.160537
PRIMER_RIGHT_2_PENALTY=0.139331
PRIMER_LEFT_2_SEQUENCE=CNCGGGGAAAAAGAAAACTAGA
PRIMER_RIGHT_2_SEQUENCE=TGGTTAGTGCAGTGTGTGTGAG
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=209,22
PRIMER_LEFT_2_TM=61.161
PRIMER_RIGHT_2_TM=59.861
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=192
PRIMER_PAIR_PENALTY_3=1.3401
PRIMER_LEFT_3_PENALTY=1.160537
PRIMER_RIGHT_3_PENALTY=0.179594
PRIMER_LEFT_3_SEQUENCE=CNCGGGGAAAAAGAAAACTAGA
PRIMER_RIGHT_3_SEQUENCE=TCTCTCCCTTCCTTGAAAATGA
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=130,22
PRIMER_LEFT_3_TM=61.161
PRIMER_RIGHT_3_TM=60.180
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=1.3982
PRIMER_LEFT_4_PENALTY=1.160537
PRIMER_RIGHT_4_PENALTY=0.237699
PRIMER_LEFT_4_SEQUENCE=CNCGGGGAAAAAGAAAACTAGA
PRIMER_RIGHT_4_SEQUENCE=CTTCCTTGAAAATGAGCCAAGT
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=123,22
PRIMER_LEFT_4_TM=61.161
PRIMER_RIGHT_4_TM=59.762
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=106
=
PRIMER_SEQUENCE_ID=SSR_05308 Ps_contig_mira-and-tgicl-ass_franssen_37126
SEQUENCE=AAAGGTTTAACTAACTAGTAAAACCTTTACCATTTCATAACAAAACAAACAAAGATTATTACTTTACACTCTTTACAGAAACAAACTACATATTGAAGAAGTCACGAATCACAATTTCTCTTCCATATTACAATCCAAATCAATGATCCTTATGATAAATAAACACTACTAGCTAGTTTACATTGCAGCAATGAACATAAACACTTACCAATAAAATAAACAGGACAAACAAATAATTATTCATACATATAAATATATATATATAAGACACCCTAATTGAAATTTCCATTCAGATGCTAACTTATAACCTAAATTAGGTTTAATTTCAATTCACAAATTTCAATTTCAATTAAGCATCATCATCATCATCATAATAATAAAAATTTACACTACCAACCATGAACAACTCTGTTGCACCTATCTTTAATCCATTTAAAACTCTTCCTAATGGAACCTTTGAGTTTACCTTCAACCGAATAAACTTTGTAACTAGCAACTCTTTTTTTCCTCTGCATCTCAGGATCACTAAAGCTCCAACTTTTCGAAGCCGAACCGTTCGTTGATTTTCCTTTCTTGAACTTAACATCGCTGATATTACTCATCTGGTTCTGTGTTTGTGCTGGATGAACAGAGGAAGCATAAGAAGCACTGTAACAACGCAGATCTTGCATACCGTAAACACTAGTAGCTGTAGCTCCATTGGAAGGTGCTGCTGCTGCTGCTGCTGAGTAGGGTTCAATTTGCATCCTTCCATCACCGTACGATTTGGATCTGAAATCTTCCATAGTTGTGAAACCCTAATTTGAGTTTCTTCAATTCAACGATTTTTGATGAATTTGTGAAGTGGTTTAGTTTGACAGTGAGAGTCTCTCTGGCTGTTACAGAGGCGCTGACTACTATCCTTTTTCCGTCGGTGCTATTTTACCCCGAC
TARGET=253,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.1275
PRIMER_LEFT_PENALTY=4.098989
PRIMER_RIGHT_PENALTY=0.028536
PRIMER_LEFT_SEQUENCE=GCAGCAATGAACATAAACACTTACC
PRIMER_RIGHT_SEQUENCE=CGGTTGAAGGTAAACTCAAAGG
PRIMER_LEFT=184,25
PRIMER_RIGHT=474,22
PRIMER_LEFT_TM=61.099
PRIMER_RIGHT_TM=60.029
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=4.1798
PRIMER_LEFT_1_PENALTY=4.098989
PRIMER_RIGHT_1_PENALTY=0.080808
PRIMER_LEFT_1_SEQUENCE=GCAGCAATGAACATAAACACTTACC
PRIMER_RIGHT_1_SEQUENCE=GTGCAACAGAGTTGTTCATGGT
PRIMER_LEFT_1=184,25
PRIMER_RIGHT_1=416,22
PRIMER_LEFT_1_TM=61.099
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=5.6174
PRIMER_LEFT_2_PENALTY=4.098989
PRIMER_RIGHT_2_PENALTY=1.518404
PRIMER_LEFT_2_SEQUENCE=GCAGCAATGAACATAAACACTTACC
PRIMER_RIGHT_2_SEQUENCE=AACAGAGTTGTTCATGGTTGGT
PRIMER_LEFT_2=184,25
PRIMER_RIGHT_2=412,22
PRIMER_LEFT_2_TM=61.099
PRIMER_RIGHT_2_TM=58.482
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=5.6732
PRIMER_LEFT_3_PENALTY=4.098989
PRIMER_RIGHT_3_PENALTY=1.574245
PRIMER_LEFT_3_SEQUENCE=GCAGCAATGAACATAAACACTTACC
PRIMER_RIGHT_3_SEQUENCE=TCGGTTGAAGGTAAACTCAAAG
PRIMER_LEFT_3=184,25
PRIMER_RIGHT_3=475,22
PRIMER_LEFT_3_TM=61.099
PRIMER_RIGHT_3_TM=58.426
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=292
PRIMER_PAIR_PENALTY_4=5.8525
PRIMER_LEFT_4_PENALTY=4.098989
PRIMER_RIGHT_4_PENALTY=1.753504
PRIMER_LEFT_4_SEQUENCE=GCAGCAATGAACATAAACACTTACC
PRIMER_RIGHT_4_SEQUENCE=CGGTTGAAGGTAAACTCAAAGGT
PRIMER_LEFT_4=184,25
PRIMER_RIGHT_4=474,23
PRIMER_LEFT_4_TM=61.099
PRIMER_RIGHT_4_TM=60.754
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_05309 Ps_contig_mira-and-tgicl-ass_franssen_37126
SEQUENCE=AAAGGTTTAACTAACTAGTAAAACCTTTACCATTTCATAACAAAACAAACAAAGATTATTACTTTACACTCTTTACAGAAACAAACTACATATTGAAGAAGTCACGAATCACAATTTCTCTTCCATATTACAATCCAAATCAATGATCCTTATGATAAATAAACACTACTAGCTAGTTTACATTGCAGCAATGAACATAAACACTTACCAATAAAATAAACAGGACAAACAAATAATTATTCATACATATAAATATATATATATAAGACACCCTAATTGAAATTTCCATTCAGATGCTAACTTATAACCTAAATTAGGTTTAATTTCAATTCACAAATTTCAATTTCAATTAAGCATCATCATCATCATCATAATAATAAAAATTTACACTACCAACCATGAACAACTCTGTTGCACCTATCTTTAATCCATTTAAAACTCTTCCTAATGGAACCTTTGAGTTTACCTTCAACCGAATAAACTTTGTAACTAGCAACTCTTTTTTTCCTCTGCATCTCAGGATCACTAAAGCTCCAACTTTTCGAAGCCGAACCGTTCGTTGATTTTCCTTTCTTGAACTTAACATCGCTGATATTACTCATCTGGTTCTGTGTTTGTGCTGGATGAACAGAGGAAGCATAAGAAGCACTGTAACAACGCAGATCTTGCATACCGTAAACACTAGTAGCTGTAGCTCCATTGGAAGGTGCTGCTGCTGCTGCTGCTGAGTAGGGTTCAATTTGCATCCTTCCATCACCGTACGATTTGGATCTGAAATCTTCCATAGTTGTGAAACCCTAATTTGAGTTTCTTCAATTCAACGATTTTTGATGAATTTGTGAAGTGGTTTAGTTTGACAGTGAGAGTCTCTCTGGCTGTTACAGAGGCGCTGACTACTATCCTTTTTCCGTCGGTGCTATTTTACCCCGAC
TARGET=355,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.1275
PRIMER_LEFT_PENALTY=4.098989
PRIMER_RIGHT_PENALTY=0.028536
PRIMER_LEFT_SEQUENCE=GCAGCAATGAACATAAACACTTACC
PRIMER_RIGHT_SEQUENCE=CGGTTGAAGGTAAACTCAAAGG
PRIMER_LEFT=184,25
PRIMER_RIGHT=474,22
PRIMER_LEFT_TM=61.099
PRIMER_RIGHT_TM=60.029
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=4.1798
PRIMER_LEFT_1_PENALTY=4.098989
PRIMER_RIGHT_1_PENALTY=0.080808
PRIMER_LEFT_1_SEQUENCE=GCAGCAATGAACATAAACACTTACC
PRIMER_RIGHT_1_SEQUENCE=GTGCAACAGAGTTGTTCATGGT
PRIMER_LEFT_1=184,25
PRIMER_RIGHT_1=416,22
PRIMER_LEFT_1_TM=61.099
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=5.6174
PRIMER_LEFT_2_PENALTY=4.098989
PRIMER_RIGHT_2_PENALTY=1.518404
PRIMER_LEFT_2_SEQUENCE=GCAGCAATGAACATAAACACTTACC
PRIMER_RIGHT_2_SEQUENCE=AACAGAGTTGTTCATGGTTGGT
PRIMER_LEFT_2=184,25
PRIMER_RIGHT_2=412,22
PRIMER_LEFT_2_TM=61.099
PRIMER_RIGHT_2_TM=58.482
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=5.6732
PRIMER_LEFT_3_PENALTY=4.098989
PRIMER_RIGHT_3_PENALTY=1.574245
PRIMER_LEFT_3_SEQUENCE=GCAGCAATGAACATAAACACTTACC
PRIMER_RIGHT_3_SEQUENCE=TCGGTTGAAGGTAAACTCAAAG
PRIMER_LEFT_3=184,25
PRIMER_RIGHT_3=475,22
PRIMER_LEFT_3_TM=61.099
PRIMER_RIGHT_3_TM=58.426
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=292
PRIMER_PAIR_PENALTY_4=5.8525
PRIMER_LEFT_4_PENALTY=4.098989
PRIMER_RIGHT_4_PENALTY=1.753504
PRIMER_LEFT_4_SEQUENCE=GCAGCAATGAACATAAACACTTACC
PRIMER_RIGHT_4_SEQUENCE=CGGTTGAAGGTAAACTCAAAGGT
PRIMER_LEFT_4=184,25
PRIMER_RIGHT_4=474,23
PRIMER_LEFT_4_TM=61.099
PRIMER_RIGHT_4_TM=60.754
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_05310 Ps_contig_mira-and-tgicl-ass_franssen_37126
SEQUENCE=AAAGGTTTAACTAACTAGTAAAACCTTTACCATTTCATAACAAAACAAACAAAGATTATTACTTTACACTCTTTACAGAAACAAACTACATATTGAAGAAGTCACGAATCACAATTTCTCTTCCATATTACAATCCAAATCAATGATCCTTATGATAAATAAACACTACTAGCTAGTTTACATTGCAGCAATGAACATAAACACTTACCAATAAAATAAACAGGACAAACAAATAATTATTCATACATATAAATATATATATATAAGACACCCTAATTGAAATTTCCATTCAGATGCTAACTTATAACCTAAATTAGGTTTAATTTCAATTCACAAATTTCAATTTCAATTAAGCATCATCATCATCATCATAATAATAAAAATTTACACTACCAACCATGAACAACTCTGTTGCACCTATCTTTAATCCATTTAAAACTCTTCCTAATGGAACCTTTGAGTTTACCTTCAACCGAATAAACTTTGTAACTAGCAACTCTTTTTTTCCTCTGCATCTCAGGATCACTAAAGCTCCAACTTTTCGAAGCCGAACCGTTCGTTGATTTTCCTTTCTTGAACTTAACATCGCTGATATTACTCATCTGGTTCTGTGTTTGTGCTGGATGAACAGAGGAAGCATAAGAAGCACTGTAACAACGCAGATCTTGCATACCGTAAACACTAGTAGCTGTAGCTCCATTGGAAGGTGCTGCTGCTGCTGCTGCTGAGTAGGGTTCAATTTGCATCCTTCCATCACCGTACGATTTGGATCTGAAATCTTCCATAGTTGTGAAACCCTAATTTGAGTTTCTTCAATTCAACGATTTTTGATGAATTTGTGAAGTGGTTTAGTTTGACAGTGAGAGTCTCTCTGGCTGTTACAGAGGCGCTGACTACTATCCTTTTTCCGTCGGTGCTATTTTACCCCGAC
TARGET=708,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1821
PRIMER_LEFT_PENALTY=0.023406
PRIMER_RIGHT_PENALTY=0.158697
PRIMER_LEFT_SEQUENCE=TAAAGCTCCAACTTTTCGAAGC
PRIMER_RIGHT_SEQUENCE=GGATGCAAATTGAACCCTACTC
PRIMER_LEFT=526,22
PRIMER_RIGHT=747,22
PRIMER_LEFT_TM=60.023
PRIMER_RIGHT_TM=59.841
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.1826
PRIMER_LEFT_1_PENALTY=0.117273
PRIMER_RIGHT_1_PENALTY=0.065292
PRIMER_LEFT_1_SEQUENCE=AGAAGCACTGTAACAACGCAGA
PRIMER_RIGHT_1_SEQUENCE=GGATAGTAGTCAGCGCCTCTGT
PRIMER_LEFT_1=641,22
PRIMER_RIGHT_1=901,22
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=59.935
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.1872
PRIMER_LEFT_2_PENALTY=0.028536
PRIMER_RIGHT_2_PENALTY=0.158697
PRIMER_LEFT_2_SEQUENCE=CCTTTGAGTTTACCTTCAACCG
PRIMER_RIGHT_2_SEQUENCE=GGATGCAAATTGAACCCTACTC
PRIMER_LEFT_2=453,22
PRIMER_RIGHT_2=747,22
PRIMER_LEFT_2_TM=60.029
PRIMER_RIGHT_2_TM=59.841
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.2760
PRIMER_LEFT_3_PENALTY=0.117273
PRIMER_RIGHT_3_PENALTY=0.158697
PRIMER_LEFT_3_SEQUENCE=AGAAGCACTGTAACAACGCAGA
PRIMER_RIGHT_3_SEQUENCE=GGATGCAAATTGAACCCTACTC
PRIMER_LEFT_3=641,22
PRIMER_RIGHT_3=747,22
PRIMER_LEFT_3_TM=60.117
PRIMER_RIGHT_3_TM=59.841
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=0.3665
PRIMER_LEFT_4_PENALTY=0.207756
PRIMER_RIGHT_4_PENALTY=0.158697
PRIMER_LEFT_4_SEQUENCE=TTTTTCCTCTGCATCTCAGGAT
PRIMER_RIGHT_4_SEQUENCE=GGATGCAAATTGAACCCTACTC
PRIMER_LEFT_4=501,22
PRIMER_RIGHT_4=747,22
PRIMER_LEFT_4_TM=60.208
PRIMER_RIGHT_4_TM=59.841
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_05311 Ps_contig_mira-and-tgicl-ass_franssen_37130
SEQUENCE=ACAAGAAGANATCTAGAGATTGGAATCATCCAAGTCTCTCTCTCTTTCTCTCTCTCTCTCTTTCGTCAATCCTTTACTACTTTATTCATTAATGCAATTATAAACAACAAACAAAGTTCAATAATACTGTCATCACTCATCGTCATCAAATTTCCAAGGGATTTTAGGGTTTTGTATTTTATTGAGAAGTAGGGGAATTTGATGATGTGATGGTGGTGGGAATGGTGCATGGGAAGTAGAATCAGGGTTAGTTTTGAAGATGAGAAGGGAGCAGAAACAGGGATGCCGAGCTTCAATTCCGACGATGCATGCTATACAGAAGGGAACACAGTTGATGGTTTTCATGTTTCTGACTTTGTAACATTTGGTCAGCCGTATCGCCTAGAGGATGCCGTTGCTCTGAGTGGAAATTCAAACTCGATTTTTAACTCATTAAAAGTAAGTGGCCAAACAATTTCTCCTGGCCCTGTTCACTTCAGTACTCTTGGAAAGTTTCCAACTTCACTTGATAAAAGCCCCTTGGAGAATCAAACGGAGCCACCTAGGTTGCAATTAACAAAGCTTCAATCATCAAACCCTAGCAGCAGTACGATATTAAGTGTTCATATAGAAAACCAGGAAGAGTTTGCCATGGCCGATGCCAGTCCTAGGACTGATATTTCTACTGATGTTGACACCGATGACAAGAATCAACGGTTTGATACAAATCAATCCCTTGCTGCTGTGGGTTCCGACTCCAGTGACAGATCAAAGGATAAGTCAGATCAAAAGACTCTCCGCAGGCTCGCTCAAAATCGTGAGGCAGCAAGAAAAAGCCGGTTGAGAAAAAAAGCCTATGTCCAACAGTTGGAAAGTAGTCGTTTGAAGCTGACTCAACTAGAGCAAGAGCTTCAGCGAGCCAGACAACAGGGAGTCTTCATATCAAGCTCGGGTGAACAAACACATTCGCTAAGCGGAAATGGGGCAATGCAATTTGATGCAGAATATGCAAGGTGGCTAGAAGAACATAATCGGCAAGTCAATGAGCTAAGAGCAGCTGTAAATTCTCATGCAAGTGATACCGAACTTCGCATGATTGTTGATGGTATATTGGCACATTATGATGAGATTTTTAGACTAAAAGGTGTTGCAGCTAAGGCTGATGTTTTCCATCTGTTGTCTGGCATGTGGAAAACACCTGCTGAGAGGTGTTTTTTATGGCTTGGTGGTTTTCGTTCGTCCGAACTTCTCAAGCTTCTGGTAAGTCAATTGGAACCTCTCACAGAGCAGCAGTTGATGGGTATTACCAACTTGCAACAATCCTCCCAACAAGCAGAAGACGCGTTGTCTCAAGGCATGGAAGCATTACAACAGTCCCTCGCCGAGACGTTGTCCACCGGCGCACCTTCCTCCTCTGGTTCCTCAGGGAATGTCGCAAATTACATGGGTCAAATGGCTATGGCCATGGGTAAGCTCGGGACACTCGAGGGCTTCATTCGACAGGCTGACAATCTGCGCCAGCAGACTCTACAACAAATGCACCGAATATTGACAACACGACAATCGGCTCGTGCACTCCTTGCCATACATGATTACTTTTCGCGGCTGCGTGCTCTCAGCTCCCTCTGGCTGGCCCGTCCAAGAGATTAATGAGTGAAGTGATTAATAGTCTGTCAAATTTTTAATTCTCACAAATCAACCACCATTGATACTTGAGTCATGATAAGTTTTGCTTCTTAACAAGTGTTGATGGTGTTGTAGTCTTTGTAATACTGATATACTAGTTGTTTCCTTGAGTTTACTAGGAATGAAATTGGAAAGGACAAGCTATTTAAATTAAAAAAGTT
TARGET=35,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05312 Ps_contig_mira-and-tgicl-ass_franssen_37130
SEQUENCE=ACAAGAAGANATCTAGAGATTGGAATCATCCAAGTCTCTCTCTCTTTCTCTCTCTCTCTCTTTCGTCAATCCTTTACTACTTTATTCATTAATGCAATTATAAACAACAAACAAAGTTCAATAATACTGTCATCACTCATCGTCATCAAATTTCCAAGGGATTTTAGGGTTTTGTATTTTATTGAGAAGTAGGGGAATTTGATGATGTGATGGTGGTGGGAATGGTGCATGGGAAGTAGAATCAGGGTTAGTTTTGAAGATGAGAAGGGAGCAGAAACAGGGATGCCGAGCTTCAATTCCGACGATGCATGCTATACAGAAGGGAACACAGTTGATGGTTTTCATGTTTCTGACTTTGTAACATTTGGTCAGCCGTATCGCCTAGAGGATGCCGTTGCTCTGAGTGGAAATTCAAACTCGATTTTTAACTCATTAAAAGTAAGTGGCCAAACAATTTCTCCTGGCCCTGTTCACTTCAGTACTCTTGGAAAGTTTCCAACTTCACTTGATAAAAGCCCCTTGGAGAATCAAACGGAGCCACCTAGGTTGCAATTAACAAAGCTTCAATCATCAAACCCTAGCAGCAGTACGATATTAAGTGTTCATATAGAAAACCAGGAAGAGTTTGCCATGGCCGATGCCAGTCCTAGGACTGATATTTCTACTGATGTTGACACCGATGACAAGAATCAACGGTTTGATACAAATCAATCCCTTGCTGCTGTGGGTTCCGACTCCAGTGACAGATCAAAGGATAAGTCAGATCAAAAGACTCTCCGCAGGCTCGCTCAAAATCGTGAGGCAGCAAGAAAAAGCCGGTTGAGAAAAAAAGCCTATGTCCAACAGTTGGAAAGTAGTCGTTTGAAGCTGACTCAACTAGAGCAAGAGCTTCAGCGAGCCAGACAACAGGGAGTCTTCATATCAAGCTCGGGTGAACAAACACATTCGCTAAGCGGAAATGGGGCAATGCAATTTGATGCAGAATATGCAAGGTGGCTAGAAGAACATAATCGGCAAGTCAATGAGCTAAGAGCAGCTGTAAATTCTCATGCAAGTGATACCGAACTTCGCATGATTGTTGATGGTATATTGGCACATTATGATGAGATTTTTAGACTAAAAGGTGTTGCAGCTAAGGCTGATGTTTTCCATCTGTTGTCTGGCATGTGGAAAACACCTGCTGAGAGGTGTTTTTTATGGCTTGGTGGTTTTCGTTCGTCCGAACTTCTCAAGCTTCTGGTAAGTCAATTGGAACCTCTCACAGAGCAGCAGTTGATGGGTATTACCAACTTGCAACAATCCTCCCAACAAGCAGAAGACGCGTTGTCTCAAGGCATGGAAGCATTACAACAGTCCCTCGCCGAGACGTTGTCCACCGGCGCACCTTCCTCCTCTGGTTCCTCAGGGAATGTCGCAAATTACATGGGTCAAATGGCTATGGCCATGGGTAAGCTCGGGACACTCGAGGGCTTCATTCGACAGGCTGACAATCTGCGCCAGCAGACTCTACAACAAATGCACCGAATATTGACAACACGACAATCGGCTCGTGCACTCCTTGCCATACATGATTACTTTTCGCGGCTGCGTGCTCTCAGCTCCCTCTGGCTGGCCCGTCCAAGAGATTAATGAGTGAAGTGATTAATAGTCTGTCAAATTTTTAATTCTCACAAATCAACCACCATTGATACTTGAGTCATGATAAGTTTTGCTTCTTAACAAGTGTTGATGGTGTTGTAGTCTTTGTAATACTGATATACTAGTTGTTTCCTTGAGTTTACTAGGAATGAAATTGGAAAGGACAAGCTATTTAAATTAAAAAAGTT
TARGET=47,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3338
PRIMER_LEFT_PENALTY=1.295461
PRIMER_RIGHT_PENALTY=0.038361
PRIMER_LEFT_SEQUENCE=TTGGAATCATCCAAGTCTCTCTC
PRIMER_RIGHT_SEQUENCE=TTTCTGCTCCCTTCTCATCTTC
PRIMER_LEFT=19,23
PRIMER_RIGHT=276,22
PRIMER_LEFT_TM=59.705
PRIMER_RIGHT_TM=59.962
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=1.3541
PRIMER_LEFT_1_PENALTY=1.295461
PRIMER_RIGHT_1_PENALTY=0.058624
PRIMER_LEFT_1_SEQUENCE=TTGGAATCATCCAAGTCTCTCTC
PRIMER_RIGHT_1_SEQUENCE=CACCATCACATCATCAAATTCC
PRIMER_LEFT_1=19,23
PRIMER_RIGHT_1=214,22
PRIMER_LEFT_1_TM=59.705
PRIMER_RIGHT_1_TM=60.059
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=196
PRIMER_PAIR_PENALTY_2=1.3541
PRIMER_LEFT_2_PENALTY=1.295461
PRIMER_RIGHT_2_PENALTY=0.058624
PRIMER_LEFT_2_SEQUENCE=TTGGAATCATCCAAGTCTCTCTC
PRIMER_RIGHT_2_SEQUENCE=CCACCATCACATCATCAAATTC
PRIMER_LEFT_2=19,23
PRIMER_RIGHT_2=215,22
PRIMER_LEFT_2_TM=59.705
PRIMER_RIGHT_2_TM=60.059
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=197
PRIMER_PAIR_PENALTY_3=1.5107
PRIMER_LEFT_3_PENALTY=1.295461
PRIMER_RIGHT_3_PENALTY=0.215225
PRIMER_LEFT_3_SEQUENCE=TTGGAATCATCCAAGTCTCTCTC
PRIMER_RIGHT_3_SEQUENCE=GATTCTACTTCCCATGCACCAT
PRIMER_LEFT_3=19,23
PRIMER_RIGHT_3=242,22
PRIMER_LEFT_3_TM=59.705
PRIMER_RIGHT_3_TM=60.215
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=1.5646
PRIMER_LEFT_4_PENALTY=1.295461
PRIMER_RIGHT_4_PENALTY=0.269153
PRIMER_LEFT_4_SEQUENCE=TTGGAATCATCCAAGTCTCTCTC
PRIMER_RIGHT_4_SEQUENCE=ATTCTACTTCCCATGCACCATT
PRIMER_LEFT_4=19,23
PRIMER_RIGHT_4=241,22
PRIMER_LEFT_4_TM=59.705
PRIMER_RIGHT_4_TM=59.731
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_05313 Ps_contig_mira-and-tgicl-ass_franssen_37136
SEQUENCE=CCGCTTCTGTTGCTGGGAGTAGTGCAGGGGAAGTTGCAATAAGGATTATTGGAGCTGGAGAGAGTATTCAAAATCAAGTTGGGGAGTTGGGTTATGATGATTGTGGGGAGGATCTGATTGGTGAGAGAAGTGGAATGTCTGCATTGGATGATGATGATGGTGGTGGTGCTGGGAGTCGTGGGGGGATTGAGGCTAGTGAGGGGGTTCCTCTTGTGTCTTCTTCGTCTTCATCGTCGTTGGCTGGAAGCGGACGGGTTGATGGTGATGCTTCTGTGAATGGATCGGAAAATAACAGCAGGGATTCATCTTCTTACCAGAGATATGATATTCAGCTTGTTGCCAAGTGGATTGAGCAGATTCTTCCTTTCTCCCTCTTGTTATTAGTTGTTTTCATCCGACAGCATTTGCAAGGTTTCTTTGTCACGATTTGGATAGCAGCTGTGATGTTCAAGTCAAATGAAATTGTTAAGAAGCAGACAGCTTTGAAGGGAGACAGGAAAGTTTCTTTGCTTGCTGGTATCTCTGTTGCTTTCATTCTTCACGTGATAGGCATTTACTGGTGGTATCGAAATGATGATATTTTATATCCATTGGTCATGTTACCTCCAAACCCAACACCTTTCT
TARGET=147,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1944
PRIMER_LEFT_PENALTY=0.156061
PRIMER_RIGHT_PENALTY=0.038361
PRIMER_LEFT_SEQUENCE=TCTGATTGGTGAGAGAAGTGGA
PRIMER_RIGHT_SEQUENCE=GGAGAAAGGAAGAATCTGCTCA
PRIMER_LEFT=112,22
PRIMER_RIGHT=370,22
PRIMER_LEFT_TM=59.844
PRIMER_RIGHT_TM=59.962
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=0.2966
PRIMER_LEFT_1_PENALTY=0.156061
PRIMER_RIGHT_1_PENALTY=0.140508
PRIMER_LEFT_1_SEQUENCE=TCTGATTGGTGAGAGAAGTGGA
PRIMER_RIGHT_1_SEQUENCE=AGGGAGAAAGGAAGAATCTGCT
PRIMER_LEFT_1=112,22
PRIMER_RIGHT_1=372,22
PRIMER_LEFT_1_TM=59.844
PRIMER_RIGHT_1_TM=59.859
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.2981
PRIMER_LEFT_2_PENALTY=0.259704
PRIMER_RIGHT_2_PENALTY=0.038361
PRIMER_LEFT_2_SEQUENCE=GAGAAGTGGAATGTCTGCATTG
PRIMER_RIGHT_2_SEQUENCE=GGAGAAAGGAAGAATCTGCTCA
PRIMER_LEFT_2=124,22
PRIMER_RIGHT_2=370,22
PRIMER_LEFT_2_TM=59.740
PRIMER_RIGHT_2_TM=59.962
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=247
PRIMER_PAIR_PENALTY_3=0.3853
PRIMER_LEFT_3_PENALTY=0.199067
PRIMER_RIGHT_3_PENALTY=0.186184
PRIMER_LEFT_3_SEQUENCE=AGGGGAAGTTGCAATAAGGATT
PRIMER_RIGHT_3_SEQUENCE=GCTGTTATTTTCCGATCCATTC
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=295,22
PRIMER_LEFT_3_TM=60.199
PRIMER_RIGHT_3_TM=59.814
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=271
PRIMER_PAIR_PENALTY_4=0.4002
PRIMER_LEFT_4_PENALTY=0.259704
PRIMER_RIGHT_4_PENALTY=0.140508
PRIMER_LEFT_4_SEQUENCE=GAGAAGTGGAATGTCTGCATTG
PRIMER_RIGHT_4_SEQUENCE=AGGGAGAAAGGAAGAATCTGCT
PRIMER_LEFT_4=124,22
PRIMER_RIGHT_4=372,22
PRIMER_LEFT_4_TM=59.740
PRIMER_RIGHT_4_TM=59.859
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_05314 Ps_contig_mira-and-tgicl-ass_franssen_37139
SEQUENCE=ATCCGCCGAATCTCGCTTTGTTGGATTCTTTATTTGGTATCCAGCGTGTTCGTCGGTCGAAGAGATTTTCTCTTGCTCTTTTCGCCGGCGGTGGAGGTTACTCTTCGACGCCGAATATCCGGCGGAGTATAAGAATCGAATGCGATAACAGCAGTGGCAGTAGTAAGAATGGAAATTCATTTGGTTATGAAAAACTTTGTGATACTATTGATTGTTCCAATGGTGTTTGTGAGCATGATCCAATTAGGTTTATGAAACCAATGTTAGGGGCGANTTTCTGTTTGCAACCTCCCGGGGATACTCCGACTCGGAGATCGACTTTCGATGCGATTCTTGTGGGTTGTATACCTGTGTTTTTCGAGGATTTGTCTGCGAAATTGCAGTATTCGTGGCATTTGCCGGAGAATGAATTTGAGAGTTTTTCTGTTACTATACCTAAGGAGGATGTTGTGTTTAAGGGATTGAGGATTCTTGATGTGTTGCAGAGAATACCTAGAGCTAGGGTTAGGAGAATGAGGGAGAAAGTAATGGAGATGATTCCTAGGGTTGTGTATAGGAAGCATAATAGTTCTCCTGGTTTGAGAACGAAGAAGGATGCATTTGATTTAACCATTGATGGTACATTGGATAAGATTCGAACGAGGCTTCAGGAACTTGATTTNGTTGTGTAATTAGNATTTTTTTCCTTTTCTTGATTTAGGTTCAGAGTTTAAGCAATAGCTGAATAGCCAATAGCATTTGTACTTTCCTTTTTAAGTTGTGTTTTTGGCAATAATCAATAGATATTGACATGGAARTTAAACTTACATTCCCTCTTAATGATTTTTTTTTCATGATCATGCCATCAAAGTGGAAAGGCAGAGCAATTACATAATATATATATTCATATTACTAAAAAAGGT
TARGET=874,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05315 Ps_contig_mira-and-tgicl-ass_franssen_37142
SEQUENCE=GGTTTACTATGAACATTTTTCCATGTTAATATCACAATGTAACCCTTTCAATTTTATTCATTACTAGAAAACAGTGGTAACCATTTTTATTTCTTCTAGTACAGAGTTACAACTTTGATGAAACTTAAATTGAGTAAGGCCAGAAACAAAAGGTTTGATCTTCAGCCGGAAACCCGAAATTACAAGAATTTTCACGAGGGTCGTCGTAGCAAACATCTTCGATCTTTGGAAGAGTCAAAAGATTATCGCTCAAATCATCGTTATCTTCTTCGTCTTGCGAGAAATCGGAGTGATCGGGTTCGAAAACATGTGAAGAATCTGTAGTAGGCTCCATGAATGAAGAAAGATTGTTCACATCATTGCAATGTGGGCTATCAGAATCAAGCACATCACTTTTAGCTGAATTAGCATCTTCATGTTTACAGTTTAGTACCATATTTGGGAATGACACTTTGGACCCATTTTCTGAATTTTCATTTGAGGTTAAGTCCATAGGTTCTTTGTTGATAGCTTCTTCTGAATTCTCTTTATCTATTGGAATCACTTTTTTCTTCAAAGAGTTCACCTCTTCTTTTAACTTGTCATTCTCTTGAACAAGATTATCATAATCATCTTTGAGTCTATCAAAGCTAGCTTTCAATGTGCCATAATCTTTTTCAAGCTGTTTGGTTTTGAACCTTGCTCTTCTATTTTGGAACCATATAGCAACTTGTCTTGGTTGTAAACCAAGATCTTTAGCAAGTTGAACTTTCCTATCAGGTTCAAGCTTGTTTTCAACCTCAAAACTTCTTTCAAGAAACTGAACTTGTTCACTTGAGAGTCTCCTTTTCTTTCCTTGTTGATGAAAACATGCTTCATAATCTTCATCACCACAATTCTCTTCTTTCTCAAGTTGTTGAAAGAAGGGTATATCTGTTACCACCCTGCTTCCACTTCCAACATTCTCAAAATCAACTATTGTCTTTGAACCTTGAAAAGAGGCAGGGTTAGAGGTATGAACCCAAAGAGATTCAAGGACTTCATTGGAGGAAGAAGGGAGAGTTTGATTTTGAAGCAAAAGTGACATATTAGAACCACCAAAAAGCTTGGCACCTGCCATAAGAACAACAGCCTTAAGAGGAAAAAACTTTTTGAAAGGATTGTAAGAGTGGAGCCTTAGGAGGGAGTTTTAGATTAAAGGGTCACAATCAAACATGGTCTAAAAGAAGTAAGAAAGAAAAAAAGAGTTGAATTTGAAAGGGTTGATTTTGATCTAGAATTTATGACGAAAGAAGCAGGTGGTGAGGATTACTATAATGTGAGAATTGTGGGAGTGAGGAGAGATCATAGTGATTAGTACTGGGAGAGATATTAAAGAGGGGTGTCAGAGGTGAATAAATTGGAGGGCAGAGAAGGTTGAGTGTAGATGTGTGAGTTTTCAAGGAAAGGCAGATGGGCAGAGAGGGGTTGAGTTAGAGAGAGAGAGAGAGTGAGATAGGAAATACTTGCTAGAGAGAGAGAG
TARGET=1454,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05316 Ps_contig_mira-and-tgicl-ass_franssen_37142
SEQUENCE=GGTTTACTATGAACATTTTTCCATGTTAATATCACAATGTAACCCTTTCAATTTTATTCATTACTAGAAAACAGTGGTAACCATTTTTATTTCTTCTAGTACAGAGTTACAACTTTGATGAAACTTAAATTGAGTAAGGCCAGAAACAAAAGGTTTGATCTTCAGCCGGAAACCCGAAATTACAAGAATTTTCACGAGGGTCGTCGTAGCAAACATCTTCGATCTTTGGAAGAGTCAAAAGATTATCGCTCAAATCATCGTTATCTTCTTCGTCTTGCGAGAAATCGGAGTGATCGGGTTCGAAAACATGTGAAGAATCTGTAGTAGGCTCCATGAATGAAGAAAGATTGTTCACATCATTGCAATGTGGGCTATCAGAATCAAGCACATCACTTTTAGCTGAATTAGCATCTTCATGTTTACAGTTTAGTACCATATTTGGGAATGACACTTTGGACCCATTTTCTGAATTTTCATTTGAGGTTAAGTCCATAGGTTCTTTGTTGATAGCTTCTTCTGAATTCTCTTTATCTATTGGAATCACTTTTTTCTTCAAAGAGTTCACCTCTTCTTTTAACTTGTCATTCTCTTGAACAAGATTATCATAATCATCTTTGAGTCTATCAAAGCTAGCTTTCAATGTGCCATAATCTTTTTCAAGCTGTTTGGTTTTGAACCTTGCTCTTCTATTTTGGAACCATATAGCAACTTGTCTTGGTTGTAAACCAAGATCTTTAGCAAGTTGAACTTTCCTATCAGGTTCAAGCTTGTTTTCAACCTCAAAACTTCTTTCAAGAAACTGAACTTGTTCACTTGAGAGTCTCCTTTTCTTTCCTTGTTGATGAAAACATGCTTCATAATCTTCATCACCACAATTCTCTTCTTTCTCAAGTTGTTGAAAGAAGGGTATATCTGTTACCACCCTGCTTCCACTTCCAACATTCTCAAAATCAACTATTGTCTTTGAACCTTGAAAAGAGGCAGGGTTAGAGGTATGAACCCAAAGAGATTCAAGGACTTCATTGGAGGAAGAAGGGAGAGTTTGATTTTGAAGCAAAAGTGACATATTAGAACCACCAAAAAGCTTGGCACCTGCCATAAGAACAACAGCCTTAAGAGGAAAAAACTTTTTGAAAGGATTGTAAGAGTGGAGCCTTAGGAGGGAGTTTTAGATTAAAGGGTCACAATCAAACATGGTCTAAAAGAAGTAAGAAAGAAAAAAAGAGTTGAATTTGAAAGGGTTGATTTTGATCTAGAATTTATGACGAAAGAAGCAGGTGGTGAGGATTACTATAATGTGAGAATTGTGGGAGTGAGGAGAGATCATAGTGATTAGTACTGGGAGAGATATTAAAGAGGGGTGTCAGAGGTGAATAAATTGGAGGGCAGAGAAGGTTGAGTGTAGATGTGTGAGTTTTCAAGGAAAGGCAGATGGGCAGAGAGGGGTTGAGTTAGAGAGAGAGAGAGAGTGAGATAGGAAATACTTGCTAGAGAGAGAGAG
TARGET=1490,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_05317 Ps_contig_mira-and-tgicl-ass_franssen_37144
SEQUENCE=AAGTGCAAAGTTGATAACTCTGTCACCGTTTCCGGTTACCTAGTTGTTTGATATGAAGATTATGCCTTCCTAGTTTGCCTCCTGTGCTTACCTCTGTAAGCTCGTATGTACTGGATGCCTCCTGGCGAACCGAGGTCCATTTGATGTGTGCAGCAAGAGGTGACTGAGTCTGAATGTAAGAATGCCTAACTTTACCCCCTTCCCCAATCACCACTTCCAAAGCAGCATTAGTCCAATAACACCCATTCTCATTCTCTTCATCATTGGAAAACTCTTCAATTATATCAACTTCTCCACCTTTCTCCACCACCACCAAAACCCTCGGATTTGAAAGATACAAAGCATTCGAACCTTCCTTACTACCCTGCAACGAAAAATAACCCAAATGAACCGGACTTTGAAGATGACAGCCCTCCGGCACATAAACCACCGTCAAATCAGGAGCACCCATACCGTTGATTGACCAAAACAAATCACCACCTTCCAATCCACAAACCAATTCCAACACCTTCGTCGTTCTTTCTTGTAACCCCCTTCCACCGTCTTCCTCCGCCGCCTCATCTTCACCTAACTCCTCCACGAATATAACTCCCTCCGGCATCG
TARGET=304,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3065
PRIMER_LEFT_PENALTY=0.210886
PRIMER_RIGHT_PENALTY=0.095574
PRIMER_LEFT_SEQUENCE=ACTTCCAAAGCAGCATTAGTCC
PRIMER_RIGHT_SEQUENCE=GATTGGAAGGTGGTGATTTGTT
PRIMER_LEFT=212,22
PRIMER_RIGHT=488,22
PRIMER_LEFT_TM=59.789
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=277
PRIMER_PAIR_PENALTY_1=0.3081
PRIMER_LEFT_1_PENALTY=0.179006
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=AGCTCGTATGTACTGGATGCCT
PRIMER_RIGHT_1_SEQUENCE=AGGAAGGTTCGAATGCTTTGTA
PRIMER_LEFT_1=98,22
PRIMER_RIGHT_1=356,22
PRIMER_LEFT_1_TM=60.179
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=259
PRIMER_PAIR_PENALTY_2=0.3400
PRIMER_LEFT_2_PENALTY=0.210886
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=ACTTCCAAAGCAGCATTAGTCC
PRIMER_RIGHT_2_SEQUENCE=AGGAAGGTTCGAATGCTTTGTA
PRIMER_LEFT_2=212,22
PRIMER_RIGHT_2=356,22
PRIMER_LEFT_2_TM=59.789
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=0.3412
PRIMER_LEFT_3_PENALTY=0.210886
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=ACTTCCAAAGCAGCATTAGTCC
PRIMER_RIGHT_3_SEQUENCE=GAAGGTGTTGGAATTGGTTTGT
PRIMER_LEFT_3=212,22
PRIMER_RIGHT_3=511,22
PRIMER_LEFT_3_TM=59.789
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.3412
PRIMER_LEFT_4_PENALTY=0.210886
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=ACTTCCAAAGCAGCATTAGTCC
PRIMER_RIGHT_4_SEQUENCE=GAAGGTGGTGATTTGTTTTGGT
PRIMER_LEFT_4=212,22
PRIMER_RIGHT_4=483,22
PRIMER_LEFT_4_TM=59.789
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_05318 Ps_contig_mira-and-tgicl-ass_franssen_37148
SEQUENCE=AGTTCTTTGATTTTCTATCTGATTTTTYYTCTAATCCAACGGCTGTAAATCGTAGTTGTGGGTCCCATTTTACGTTAACAAAAGACATGATTGAAGCTAAATTCATTGACCTCTCTTGATGAAAAATCAACGGTCGTGATCAATTTCTATCTTCTTCTATAAAACTCCATAATCATGTGAGTACCTTTTTGAACTAGTTCCAGATTTATACTCAGAGTTTTAGGGTTGAACATAACCAGATTCATTGCCATAAGTCCATAACCAGAATTGCAACAATGCTTCTACGAAGCTCGTCAACGCCGGTTATGAATCCACGGCTTCCACACTCAAACTCGAAGGATTCATCTCCCGAACCGGAATTTTTTCACCGAAACCCGAAATCCAGATCTGTTACGTTCTCGCCTTCGTCGTCGAGTTCACTGTCACCGATTACTGGTTCAATGAACAAACTAACTCGAGCACTTTCGGAGACCGATCTCTCAGTGCCGCCTAATCGGAAGCCGTTCCACCGCCGTCAATTTGATGATGACGATGAAGAGAGCAGAACTCTCGGTTTTGGTGCTCGTTCCAGATCGGCGTCGTTTAGTTCCGCGTTGTGTTCTTTATCTGAGTCGGAAGAAAGCCAGAGTGGAGTCGATGCGAGTGACAGCGGTGTGTTGGTGGAAGAAGGCGGCGGCGGTGGTGGATTCGACAAAAACGATGGTGGAGTTTCTAGATTCGGTGATTCAAATCACGGAAACGATAGTACCGATTTGTATTACCGTACAATGATCGAAGCTAATCCAGGGAATCCTCTTTTTCTTAGCAACTACGCAAAGTACTTGAAAGAGGTTCGTAAGGATTATGTGAAAGCTGAAGAGTATTGTGGAAGGGCAATATTGGCAAATCCAAATGATGGTAACGTTCTGTCACTTTACGCAGATTTGATATGGGAGTGTCATAAGGATGCTTCTCGAGCTGAGACTTACTTTGATCAAGCAGTTAAAGCAGCTCCTGATGACTGTTATGTTTTAGCGTCGTATGCGCATTTTCTATGGGATGCTGAGGAAGAAGAAGAGGATGAAGTTGAAGAAGAATCCTCTGAAAAAGCTTTTGGTTTCTTTAATGGAGGTTCTCTATCAACAACTCCTCCGCCGTTGGCTGCTGCTGCTTCTTGAATTCTGTCCTTTTGTGGTTTCTGGGTTTCAGTTTTATGAGTTTTTCTAACCTATGTTTATATTAATTGTTATTATTATGATTAGCTTAACCTTTTCTATTAAATTTTTGGCCGATGAATATAGATGTAATAATGGTCTTTGTTCGCCTTACCATAAATGTTGAAATAAGAATAAAAGGTGGGGCTTTTGAATGAATTGAATTGTTAAATACTAAAAACTAG
TARGET=1140,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05319 Ps_contig_mira-and-tgicl-ass_franssen_37152
SEQUENCE=AGTTTTTTTGTTGTATAGTTCTTATTGTTAAATTAAAAAAAAAATTAAAGTAATTTTTAAGTTTACTGCCATTACTAAAAAGTAAGAACAAGAAGCCAAATCATAAATACTATAACTGCAACCACGTCAACAAAGAAAAGAGGTTTACCACCAAATGCCAAAGCCTCATGGAATTTAGGAATAACTAGTTACAGAATTATAAATTTTAATCAATTACTAGAATACAGGAAGTGTAATTCTCTGGTAGGAGTAAAAATATACACTAGACTCCTGTGTGAATGTACATTAACATCTGCTTAAATTGCTCTTCTTTTGGAAATATGTGGCGGCAAAGTTACCGTCCAGCGAGTTTGACCATCCATGCACCACCCAAGTAAAGGCCTAATAAGGGCGCCGCTAGAAGAACTTGAGTCAGAGGATCTGTCGATGGTGTAACTACAGCTGCTGCCACTACTGCGCCGACTACAACATACCTCCATACCGATAGCATCTTGTCTCCAGATACCAATCCAAGTTGTCCCAATAGAAGTTGTATAATAGGCACCTGGAAAGACAAGCCTGTGCTGAACATAAGAACCAGAACAAACTCAAAGTATTGATCAATGGACCATAATGATTCAACAGCGCCTTCGGCGTAATTGACAAAGAAATTTAATGCCGCAGGTACCAAAACTAAGTAGGAGAAGGTTATTCCGGCATAAAAAAGAACGGATGAGCCTAAAACAATTGGCCCGAGAAACTTTCTTTCTTCTTTCGTTAGACCTGGAATTATAAAGGCTATGATTTCGTAGAGAATGATGGGACTTCCCAAGAGGAGGCCACAGTATCCAGACACCTTTAAAGTGGTGAAGAAAAATTCACCAGGAGCAAGCTGAAGAAATCGGACACCTTCTGATTTAACAGGAGCTTCGAGAATCTTTACCAAATCTTTTGAAAACGCAAAGCATCCCAAAATGCTTCCTCCAACTCCCAGAACTGATATAAAGATTCTCTCTCGTAGCTCTTCAAGGTGATCAAATATGCTCATCTCTTTATCATCGGGTAGAAGCTCTTTACTAGGATAAAGAAAATCGTAGATAGCACTTCTTTCCCCATCTTCCTTAAAATTTTCCAATGCTTCTTCACTTATACCATCAATCATATCTTTATTCTCGGGTCTTTCTTCAACGGCGGAGCCAAGTCTAGTGGAACTAGTACTCAACTGTTGTTGTTGCTTCTCTCTGATTTCGTCATCAACGGCGAAACAAACGAGTCTATCAAAACTCTTGTTCTTTCTTAGAGAGAGAGGGTAGCTGAACCCGGTCGAATTGTTGACCCGGATGGGGTTTAAGTGGGTTCGGTGCAACCCGAATTGAGGAAGGATGTTCGTAGGAACAGTGGTGGTTCCCAAACCCATACCTCGGCCACGTACGGTGTTTGTGCTTTTGTTTTAGCGTCGAACGGTTGGTTCTCGTTAGATACGATTTGGTTTGCGTGTTGTACTGTGTCTTAGCCCAGATATTTTGCTCTCGCAATTGTTACTTACGTATACACTGCAATCTAGTATTGTT
TARGET=1278,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1525
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=0.020269
PRIMER_LEFT_SEQUENCE=TTGCTTCTCTCTGATTTCGTCA
PRIMER_RIGHT_SEQUENCE=CACGCAAACCAAATCGTATCTA
PRIMER_LEFT=1210,22
PRIMER_RIGHT=1475,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=60.020
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.1697
PRIMER_LEFT_1_PENALTY=0.132262
PRIMER_RIGHT_1_PENALTY=0.037486
PRIMER_LEFT_1_SEQUENCE=TTGCTTCTCTCTGATTTCGTCA
PRIMER_RIGHT_1_SEQUENCE=CAGTACAACACGCAAACCAAAT
PRIMER_LEFT_1=1210,22
PRIMER_RIGHT_1=1483,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=59.963
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=274
PRIMER_PAIR_PENALTY_2=0.3885
PRIMER_LEFT_2_PENALTY=0.368190
PRIMER_RIGHT_2_PENALTY=0.020269
PRIMER_LEFT_2_SEQUENCE=TTGTTGCTTCTCTCTGATTTCG
PRIMER_RIGHT_2_SEQUENCE=CACGCAAACCAAATCGTATCTA
PRIMER_LEFT_2=1207,22
PRIMER_RIGHT_2=1475,22
PRIMER_LEFT_2_TM=59.632
PRIMER_RIGHT_2_TM=60.020
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.0000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=0.6061
PRIMER_LEFT_3_PENALTY=0.132262
PRIMER_RIGHT_3_PENALTY=0.473868
PRIMER_LEFT_3_SEQUENCE=TTGCTTCTCTCTGATTTCGTCA
PRIMER_RIGHT_3_SEQUENCE=ATCTAACGAGAACCAACCGTTC
PRIMER_LEFT_3=1210,22
PRIMER_RIGHT_3=1458,22
PRIMER_LEFT_3_TM=60.132
PRIMER_RIGHT_3_TM=59.526
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.6139
PRIMER_LEFT_4_PENALTY=0.593677
PRIMER_RIGHT_4_PENALTY=0.020269
PRIMER_LEFT_4_SEQUENCE=GCGAAACAAACGAGTCTATCAA
PRIMER_RIGHT_4_SEQUENCE=CACGCAAACCAAATCGTATCTA
PRIMER_LEFT_4=1238,22
PRIMER_RIGHT_4=1475,22
PRIMER_LEFT_4_TM=59.406
PRIMER_RIGHT_4_TM=60.020
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_05320 Ps_contig_mira-and-tgicl-ass_franssen_37153
SEQUENCE=CAACGCAGAGTACGCGGGGACACGAGGAGAGCAATTATTATGGTTCTTGTACAATGTAACAAAAATAATAAGTAATAACAATNATATAAATATTTGAAGAATAGTGGAAGGTGGGTGAAGCAAGGAAAAGCACTTTCGGCCGCCGGTGGATGCCGATACAGATGCTTCATCCATCCACTATCACATAAAACTATTTCTTCCACTTTTCTTCTTCTTCCTCCAAAATTCACCACATAAAACTCTCTTTCTCATTCTGCTACCACTTCTACATAAAAACAACCTAGTACACATCAACATATACAACAATAATAATAATAATGTTGTTTAAATTTGTAGCAGGTCCTAGGAAGAAAAAACCAGGAACCGTTCCTGTTTACCTAAACGTCTATGACCTCACACCTATTAACGGTTATGCTTACTGGTTTGGTCTTGGAATCTACCATTCAGGTGTTCAAGTTCATGATGTTGAATATGGTTTTGGGGCACATGAAAATGACACAACGGGTATTTTTGAAGTGCAACCGAAAAACTGTCCTGGTTTCACTTTCAGAAAATCGATTTTCATTGGAACAACGGATCTGGGTAGTAAAGATGTTGGTGTTTTCATGGAGAAACTAGCTCGAGAATATTCTGGAAATTCTTATCATCTTATTTCCAAGAATTGTAACCATTTTTGTGATGATGTTTGTTACAAATTAACGGGGAAATCGATTCCGAGATGGGTTAATCGACTTGCCAGACTTTGGTTTATTTTGCAACTGTGTTGTTCCTCCCGCCCGGTCTAAAACGAAACAAAGGTTCGACAAGTTACGTCCGACAAGGTTNAAGAAGGTGAAAAACGGAAA
TARGET=206,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2589
PRIMER_LEFT_PENALTY=0.037936
PRIMER_RIGHT_PENALTY=0.220992
PRIMER_LEFT_SEQUENCE=TGCTTCATCCATCCACTATCAC
PRIMER_RIGHT_SEQUENCE=GGTAGATTCCAAGACCAAACCA
PRIMER_LEFT=162,22
PRIMER_RIGHT=440,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=60.221
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.2589
PRIMER_LEFT_1_PENALTY=0.037936
PRIMER_RIGHT_1_PENALTY=0.220992
PRIMER_LEFT_1_SEQUENCE=TGCTTCATCCATCCACTATCAC
PRIMER_RIGHT_1_SEQUENCE=TGGTAGATTCCAAGACCAAACC
PRIMER_LEFT_1=162,22
PRIMER_RIGHT_1=441,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=60.221
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.2589
PRIMER_LEFT_2_PENALTY=0.037936
PRIMER_RIGHT_2_PENALTY=0.220992
PRIMER_LEFT_2_SEQUENCE=GCTTCATCCATCCACTATCACA
PRIMER_RIGHT_2_SEQUENCE=GGTAGATTCCAAGACCAAACCA
PRIMER_LEFT_2=163,22
PRIMER_RIGHT_2=440,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=60.221
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.2589
PRIMER_LEFT_3_PENALTY=0.037936
PRIMER_RIGHT_3_PENALTY=0.220992
PRIMER_LEFT_3_SEQUENCE=GCTTCATCCATCCACTATCACA
PRIMER_RIGHT_3_SEQUENCE=TGGTAGATTCCAAGACCAAACC
PRIMER_LEFT_3=163,22
PRIMER_RIGHT_3=441,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=60.221
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.2589
PRIMER_LEFT_4_PENALTY=0.037936
PRIMER_RIGHT_4_PENALTY=0.220992
PRIMER_LEFT_4_SEQUENCE=TACAGATGCTTCATCCATCCAC
PRIMER_RIGHT_4_SEQUENCE=GGTAGATTCCAAGACCAAACCA
PRIMER_LEFT_4=156,22
PRIMER_RIGHT_4=440,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=60.221
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_05321 Ps_contig_mira-and-tgicl-ass_franssen_37153
SEQUENCE=CAACGCAGAGTACGCGGGGACACGAGGAGAGCAATTATTATGGTTCTTGTACAATGTAACAAAAATAATAAGTAATAACAATNATATAAATATTTGAAGAATAGTGGAAGGTGGGTGAAGCAAGGAAAAGCACTTTCGGCCGCCGGTGGATGCCGATACAGATGCTTCATCCATCCACTATCACATAAAACTATTTCTTCCACTTTTCTTCTTCTTCCTCCAAAATTCACCACATAAAACTCTCTTTCTCATTCTGCTACCACTTCTACATAAAAACAACCTAGTACACATCAACATATACAACAATAATAATAATAATGTTGTTTAAATTTGTAGCAGGTCCTAGGAAGAAAAAACCAGGAACCGTTCCTGTTTACCTAAACGTCTATGACCTCACACCTATTAACGGTTATGCTTACTGGTTTGGTCTTGGAATCTACCATTCAGGTGTTCAAGTTCATGATGTTGAATATGGTTTTGGGGCACATGAAAATGACACAACGGGTATTTTTGAAGTGCAACCGAAAAACTGTCCTGGTTTCACTTTCAGAAAATCGATTTTCATTGGAACAACGGATCTGGGTAGTAAAGATGTTGGTGTTTTCATGGAGAAACTAGCTCGAGAATATTCTGGAAATTCTTATCATCTTATTTCCAAGAATTGTAACCATTTTTGTGATGATGTTTGTTACAAATTAACGGGGAAATCGATTCCGAGATGGGTTAATCGACTTGCCAGACTTTGGTTTATTTTGCAACTGTGTTGTTCCTCCCGCCCGGTCTAAAACGAAACAAAGGTTCGACAAGTTACGTCCGACAAGGTTNAAGAAGGTGAAAAACGGAAA
TARGET=305,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2589
PRIMER_LEFT_PENALTY=0.037936
PRIMER_RIGHT_PENALTY=0.220992
PRIMER_LEFT_SEQUENCE=TGCTTCATCCATCCACTATCAC
PRIMER_RIGHT_SEQUENCE=GGTAGATTCCAAGACCAAACCA
PRIMER_LEFT=162,22
PRIMER_RIGHT=440,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=60.221
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.2589
PRIMER_LEFT_1_PENALTY=0.037936
PRIMER_RIGHT_1_PENALTY=0.220992
PRIMER_LEFT_1_SEQUENCE=TGCTTCATCCATCCACTATCAC
PRIMER_RIGHT_1_SEQUENCE=TGGTAGATTCCAAGACCAAACC
PRIMER_LEFT_1=162,22
PRIMER_RIGHT_1=441,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=60.221
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.2589
PRIMER_LEFT_2_PENALTY=0.037936
PRIMER_RIGHT_2_PENALTY=0.220992
PRIMER_LEFT_2_SEQUENCE=GCTTCATCCATCCACTATCACA
PRIMER_RIGHT_2_SEQUENCE=GGTAGATTCCAAGACCAAACCA
PRIMER_LEFT_2=163,22
PRIMER_RIGHT_2=440,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=60.221
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.2589
PRIMER_LEFT_3_PENALTY=0.037936
PRIMER_RIGHT_3_PENALTY=0.220992
PRIMER_LEFT_3_SEQUENCE=GCTTCATCCATCCACTATCACA
PRIMER_RIGHT_3_SEQUENCE=TGGTAGATTCCAAGACCAAACC
PRIMER_LEFT_3=163,22
PRIMER_RIGHT_3=441,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=60.221
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.2589
PRIMER_LEFT_4_PENALTY=0.037936
PRIMER_RIGHT_4_PENALTY=0.220992
PRIMER_LEFT_4_SEQUENCE=TACAGATGCTTCATCCATCCAC
PRIMER_RIGHT_4_SEQUENCE=GGTAGATTCCAAGACCAAACCA
PRIMER_LEFT_4=156,22
PRIMER_RIGHT_4=440,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=60.221
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=285
=
PRIMER_SEQUENCE_ID=SSR_05322 Ps_contig_mira-and-tgicl-ass_franssen_37160
SEQUENCE=GTTCGGGGTAAACCTATATAAAATCCGTTTTCTCTCTCACCNTCGAATGACTCTCATTTTCTCATCCCTCTATCAACAAAATGAAGAAAAGGGTTAAATAGTAAATACAACAAAAGATCCTTTCCTATTTCCACCTTCTCTTCCATAGAGAAAGAAAAAACTGGAAAGAGATTTAGATGGATGAAGAAAGACTAGTAAAGCTACTGTTATTTCTTTCTATCCTACTAAACGCTGCGTTTAAACAGTGTCTATCAGATCCAAGCGACGAAACATGTCTAACAGAATTAAGCAAATCATTACAAGACCCAAACAAATCACTCCACAACTGGAACGAACAAACCTTCGCTAAACCCTGCAACGAATCAACCTCAAATCTCCAAGGCGCAATCTGCAACAATGGCCGAATCTACAAACTCTCCCTCAACAACCTCTCTCTCCGCGGCACCATTTCACCTTTCCTCTCCAACTGCACAAATCTCCAAACCCTAGACCTATCTTCCAACTTCCTCACCGGACCAATCCCGCCGGATTTACAATCACTCGTCAATCTCGCCGTTCTGAATCTCTCGTCTAACCGTCTCGAAGGTGAGATTCCACCTCAGCTCACATTGTGTGCTTATCTCAACGTAATCGATTTTCATGATAATCTTCTCACCGGTCCGATTCCTCAGCAATTAGGTTTATTAGTTCGATTATCGGCTTTCGATGTTTCGAATAACCGACTCTCCGGTCCGATCCCGTTTTCGTTGTCGAACCGGAGTAGTCCGAATTTGCCGAAATTTAACGCGAGTTCGTTTGAAGGGAACAAGGATCTTTACGGTTATCCGTTACCGCCGATGAGGAGTAAAGGTTTATCGATTTTGGCGATTGTTGGAATCGGATTAGGAAGTGGATTAGCGAGTCTTGTTTTGAGTTTTACTGGTGTTTGTATTTGGTTAAAAGTTACTGATCGGAAAGTTGCATTAGAAGAAGGCAAAATTAGTCATCTAATGCCTGATTATTGAATGTTAATTATTATTATTATGATAGCATTTACAATTTTNNNAYCTAAGGTGTT
TARGET=1011,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05323 Ps_contig_mira-and-tgicl-ass_franssen_37164
SEQUENCE=TGGTTTGGTRAAAATAAATCAAGGGNTTNGGATTCAATAATGGTGGTGGAAGAGATGATGGTGGCAGTGCTAGGATTTTGGGAAATCTTGCTTTGGCTATTGGGTTAACTTATCTTTCAGTGACTGGACAACTTGGATGGATTTTGGACGCTATTGTCTCCATTTGGATCTTTGTAGTGTTAGTACCGATAGTAGGTTTTGGTGCTTTCCTCTGGTGGGCAGGAAGAGATATAATGAAAGGCACTTGTCCAAACTGTGGAAATGATTTTCAGGTTTTCAAATCCACTTTAAATGAGGAATTGCAGTTGTGCCCCTTTTGCGGTCAACCTTTTTCTGTTGATGGCAATGAGTTTGTAAAGGAATCTGTAAAGTTTTCAAACCAATCTACAACATTTGGACAAGCATTCGACAATTTCTCGCGTTCTAGAAATGACGAGGATTCCGGTAGAGCAATTGATGTTGAAGCTGAAATAAAAGACGCGGATTGAGTCTTTGCTGAGTGTGTCCTTATTATTCTGAAAATATATTGTTGGTGCTGTTTCTTGCTCTAATAACTGTCTCTCTCTCTCTGTGCCACTAAATAACCTGTATGAGATTTGTAATTTTATCTCATTTTTCAGGTTAACTATGCTGAAGTGAAGTCTCATTCACTTTAGCACATACTAGAGGGAAGAGTGTAGTAGTGTGCAAGCATGTCAACACATTTTTAGATATGATAGGAATATAGTGAGTTGAGTAGCATTACAATTGTAATATAAGTTTTGATAGTGATGTTTCTCTTTCTCACATCACACATCTATTAACAAAAATGCGTCTTCAATTGTTAAATTTTTAATTTATT
TARGET=558,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05324 Ps_contig_mira-and-tgicl-ass_franssen_37167
SEQUENCE=ACCATACTCACATGATATATATAAACATTCTCATATCTCTTCTATAACCACATTCAACTTTTGCATCAATATTCATATATAATCACAATAACATATTTATGACTGTGATGAAAATTATGTGGAAATCAATATTTCCAAGCTGTTACAAGGCTGAAGATACTGAATACTCTTCGGCCAAAGAAACAACAAAAGTGGTTGCTACAAAGACAAGTTCTTTCAACAGAATCTCTCTCACGGATCTGAGTTTTCCAAGTGCTACAACACTTTCTGAAGATCTTTCAATTTCTTTAGCTGGTTCTAATCTCTATGTTTTCACACTTGCTGAGCTCAAGATTATTACTCAGAGTTTCTCTTCAACGAATTTTCTTGGTGAAGGAGGGTTTGGTCCTGTTCATAAAGGTTTCATTGATGATAAGGTTAGGCATGGACTTGAACCTCAACCTGTGGCTGTTAAGCTCCTAGATTTGGATGGCTCTCAGGGTCATAAAGAATGGCTGACTGAGGTTGTGTTTCTTGGTCAACTGAGACATGAACACCTTGTGAAGCTGATTGGATATTGTTGTGAAGAAGAACACAGGCTTTTGGTTTATGAGTATTTACCAAGAGGAAGCTTGGAAAATCAACTATTTAGAAGATATTCAGCATCGTTACCATGGTCAACAAGAATGAAAATTGCTGTTGGAGCTGCAAAAGGTTTAGCCTTTTTACATGATGCAAAGAAACCAGTCATCTATAGAGATTTCAAAGCCTCAAACATCTTATTAGACTCGGACTATAATGCAAAACTCTCTGATTTTGGTTTGGCAAAAGATGGCCCTGAAGGTGATGACACACATGTTTCAACTAGAGTTATGGGTACTCAAGGTTACGCCGCACCCGAATATATCATGACAGGACATTTGACAGCAATGAGTGATGTGTATAGTTTTGGAGTTGTCCTATTGGAGCTACTAACCGGAAGAAGATCAGTGGACAAGGCACGTCCACCACGAGAACACAATCTAGTGGAATGGGCCAGACCGGTTTTAAACGACGCTCGTAAGCTCACCAGAATAATGGATCCAAGGCTTGAAGGACAGTATTCAGAAATGGGAGCAAGAAAAGCAGCTGCATTAGCTTATCAATGTCTTAGCCATAGACCAAGAAACCGACCTACAATGTCTACCGTAGTGAACACTCTTGAACCTCTTCAAGATTTCGACGATATACCAATAGGTCCATTTGTTTACACAGCTCCGAGTGATGTTAGTAATAATGAAGTGAGGAACGAGAAAACAACATCGATACACCAAAGGAGAGAAAAAGAGAAAGTACTCATCATCATCATCGTAGAAATCAAAATGGTAGTAATAATAATAATGAAGTAGTAGTACATAAGAAAGATGGTGAAGAATATTATGATAATCCTAAAGAGAAGAAAAGGGAAAGTAGTGAGAATCATAGAAGACATCATCATCATCGTCAGAATGGACATAGGCATCCATTAAAGTCACCAAAAGAACAAAGACATGTACATCAAAGTGGGTCACACTCACCTGATACATCCATTACATCAGAAAGCCAAGGAAATTAAGGAACTATAGTTTAGAAGACACGTACCTAGATCAAACACTGCTAAATGTATATATATTGTGTCAATGTCAATGAGCTGGTTTCAAACAAATAAAAGTTGAAAACAAAGAAAAAGGGAGAGGGAAGTGATAATGCAATTATGCAATGGTCCTAGCATAGAAAGCATGCTTGTANTTTTTATAATACTTATTTTTGTATTTCTNGAGGTAAGTTGATTTATTTTAGAATTTGTGTAATACAAATTATTTTAAAATTTAAA
TARGET=1315,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1535
PRIMER_LEFT_PENALTY=0.108684
PRIMER_RIGHT_PENALTY=0.044832
PRIMER_LEFT_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_SEQUENCE=CCTATGTCCATTCTGACGATGA
PRIMER_LEFT=1255,22
PRIMER_RIGHT=1476,22
PRIMER_LEFT_TM=59.891
PRIMER_RIGHT_TM=59.955
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.2420
PRIMER_LEFT_1_PENALTY=0.197140
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_LEFT_1_SEQUENCE=TGTTTACACAGCTCCGAGTGAT
PRIMER_RIGHT_1_SEQUENCE=CCTATGTCCATTCTGACGATGA
PRIMER_LEFT_1=1222,22
PRIMER_RIGHT_1=1476,22
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=0.4156
PRIMER_LEFT_2_PENALTY=0.108684
PRIMER_RIGHT_2_PENALTY=0.306868
PRIMER_LEFT_2_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_2_SEQUENCE=AATGGATGCCTATGTCCATTCT
PRIMER_LEFT_2=1255,22
PRIMER_RIGHT_2=1484,22
PRIMER_LEFT_2_TM=59.891
PRIMER_RIGHT_2_TM=59.693
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.4570
PRIMER_LEFT_3_PENALTY=0.108684
PRIMER_RIGHT_3_PENALTY=0.348266
PRIMER_LEFT_3_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_3_SEQUENCE=ATGTCCATTCTGACGATGATGA
PRIMER_LEFT_3=1255,22
PRIMER_RIGHT_3=1473,22
PRIMER_LEFT_3_TM=59.891
PRIMER_RIGHT_3_TM=60.348
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=219
PRIMER_PAIR_PENALTY_4=0.4570
PRIMER_LEFT_4_PENALTY=0.108684
PRIMER_RIGHT_4_PENALTY=0.348266
PRIMER_LEFT_4_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_4_SEQUENCE=GTCCATTCTGACGATGATGATG
PRIMER_LEFT_4=1255,22
PRIMER_RIGHT_4=1471,22
PRIMER_LEFT_4_TM=59.891
PRIMER_RIGHT_4_TM=60.348
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_05325 Ps_contig_mira-and-tgicl-ass_franssen_37167
SEQUENCE=ACCATACTCACATGATATATATAAACATTCTCATATCTCTTCTATAACCACATTCAACTTTTGCATCAATATTCATATATAATCACAATAACATATTTATGACTGTGATGAAAATTATGTGGAAATCAATATTTCCAAGCTGTTACAAGGCTGAAGATACTGAATACTCTTCGGCCAAAGAAACAACAAAAGTGGTTGCTACAAAGACAAGTTCTTTCAACAGAATCTCTCTCACGGATCTGAGTTTTCCAAGTGCTACAACACTTTCTGAAGATCTTTCAATTTCTTTAGCTGGTTCTAATCTCTATGTTTTCACACTTGCTGAGCTCAAGATTATTACTCAGAGTTTCTCTTCAACGAATTTTCTTGGTGAAGGAGGGTTTGGTCCTGTTCATAAAGGTTTCATTGATGATAAGGTTAGGCATGGACTTGAACCTCAACCTGTGGCTGTTAAGCTCCTAGATTTGGATGGCTCTCAGGGTCATAAAGAATGGCTGACTGAGGTTGTGTTTCTTGGTCAACTGAGACATGAACACCTTGTGAAGCTGATTGGATATTGTTGTGAAGAAGAACACAGGCTTTTGGTTTATGAGTATTTACCAAGAGGAAGCTTGGAAAATCAACTATTTAGAAGATATTCAGCATCGTTACCATGGTCAACAAGAATGAAAATTGCTGTTGGAGCTGCAAAAGGTTTAGCCTTTTTACATGATGCAAAGAAACCAGTCATCTATAGAGATTTCAAAGCCTCAAACATCTTATTAGACTCGGACTATAATGCAAAACTCTCTGATTTTGGTTTGGCAAAAGATGGCCCTGAAGGTGATGACACACATGTTTCAACTAGAGTTATGGGTACTCAAGGTTACGCCGCACCCGAATATATCATGACAGGACATTTGACAGCAATGAGTGATGTGTATAGTTTTGGAGTTGTCCTATTGGAGCTACTAACCGGAAGAAGATCAGTGGACAAGGCACGTCCACCACGAGAACACAATCTAGTGGAATGGGCCAGACCGGTTTTAAACGACGCTCGTAAGCTCACCAGAATAATGGATCCAAGGCTTGAAGGACAGTATTCAGAAATGGGAGCAAGAAAAGCAGCTGCATTAGCTTATCAATGTCTTAGCCATAGACCAAGAAACCGACCTACAATGTCTACCGTAGTGAACACTCTTGAACCTCTTCAAGATTTCGACGATATACCAATAGGTCCATTTGTTTACACAGCTCCGAGTGATGTTAGTAATAATGAAGTGAGGAACGAGAAAACAACATCGATACACCAAAGGAGAGAAAAAGAGAAAGTACTCATCATCATCATCGTAGAAATCAAAATGGTAGTAATAATAATAATGAAGTAGTAGTACATAAGAAAGATGGTGAAGAATATTATGATAATCCTAAAGAGAAGAAAAGGGAAAGTAGTGAGAATCATAGAAGACATCATCATCATCGTCAGAATGGACATAGGCATCCATTAAAGTCACCAAAAGAACAAAGACATGTACATCAAAGTGGGTCACACTCACCTGATACATCCATTACATCAGAAAGCCAAGGAAATTAAGGAACTATAGTTTAGAAGACACGTACCTAGATCAAACACTGCTAAATGTATATATATTGTGTCAATGTCAATGAGCTGGTTTCAAACAAATAAAAGTTGAAAACAAAGAAAAAGGGAGAGGGAAGTGATAATGCAATTATGCAATGGTCCTAGCATAGAAAGCATGCTTGTANTTTTTATAATACTTATTTTTGTATTTCTNGAGGTAAGTTGATTTATTTTAGAATTTGTGTAATACAAATTATTTTAAAATTTAAA
TARGET=1348,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1535
PRIMER_LEFT_PENALTY=0.108684
PRIMER_RIGHT_PENALTY=0.044832
PRIMER_LEFT_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_SEQUENCE=CCTATGTCCATTCTGACGATGA
PRIMER_LEFT=1255,22
PRIMER_RIGHT=1476,22
PRIMER_LEFT_TM=59.891
PRIMER_RIGHT_TM=59.955
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.2420
PRIMER_LEFT_1_PENALTY=0.197140
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_LEFT_1_SEQUENCE=TGTTTACACAGCTCCGAGTGAT
PRIMER_RIGHT_1_SEQUENCE=CCTATGTCCATTCTGACGATGA
PRIMER_LEFT_1=1222,22
PRIMER_RIGHT_1=1476,22
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=0.4156
PRIMER_LEFT_2_PENALTY=0.108684
PRIMER_RIGHT_2_PENALTY=0.306868
PRIMER_LEFT_2_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_2_SEQUENCE=AATGGATGCCTATGTCCATTCT
PRIMER_LEFT_2=1255,22
PRIMER_RIGHT_2=1484,22
PRIMER_LEFT_2_TM=59.891
PRIMER_RIGHT_2_TM=59.693
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.4570
PRIMER_LEFT_3_PENALTY=0.108684
PRIMER_RIGHT_3_PENALTY=0.348266
PRIMER_LEFT_3_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_3_SEQUENCE=ATGTCCATTCTGACGATGATGA
PRIMER_LEFT_3=1255,22
PRIMER_RIGHT_3=1473,22
PRIMER_LEFT_3_TM=59.891
PRIMER_RIGHT_3_TM=60.348
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=219
PRIMER_PAIR_PENALTY_4=0.4570
PRIMER_LEFT_4_PENALTY=0.108684
PRIMER_RIGHT_4_PENALTY=0.348266
PRIMER_LEFT_4_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_4_SEQUENCE=GTCCATTCTGACGATGATGATG
PRIMER_LEFT_4=1255,22
PRIMER_RIGHT_4=1471,22
PRIMER_LEFT_4_TM=59.891
PRIMER_RIGHT_4_TM=60.348
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_05326 Ps_contig_mira-and-tgicl-ass_franssen_37167
SEQUENCE=ACCATACTCACATGATATATATAAACATTCTCATATCTCTTCTATAACCACATTCAACTTTTGCATCAATATTCATATATAATCACAATAACATATTTATGACTGTGATGAAAATTATGTGGAAATCAATATTTCCAAGCTGTTACAAGGCTGAAGATACTGAATACTCTTCGGCCAAAGAAACAACAAAAGTGGTTGCTACAAAGACAAGTTCTTTCAACAGAATCTCTCTCACGGATCTGAGTTTTCCAAGTGCTACAACACTTTCTGAAGATCTTTCAATTTCTTTAGCTGGTTCTAATCTCTATGTTTTCACACTTGCTGAGCTCAAGATTATTACTCAGAGTTTCTCTTCAACGAATTTTCTTGGTGAAGGAGGGTTTGGTCCTGTTCATAAAGGTTTCATTGATGATAAGGTTAGGCATGGACTTGAACCTCAACCTGTGGCTGTTAAGCTCCTAGATTTGGATGGCTCTCAGGGTCATAAAGAATGGCTGACTGAGGTTGTGTTTCTTGGTCAACTGAGACATGAACACCTTGTGAAGCTGATTGGATATTGTTGTGAAGAAGAACACAGGCTTTTGGTTTATGAGTATTTACCAAGAGGAAGCTTGGAAAATCAACTATTTAGAAGATATTCAGCATCGTTACCATGGTCAACAAGAATGAAAATTGCTGTTGGAGCTGCAAAAGGTTTAGCCTTTTTACATGATGCAAAGAAACCAGTCATCTATAGAGATTTCAAAGCCTCAAACATCTTATTAGACTCGGACTATAATGCAAAACTCTCTGATTTTGGTTTGGCAAAAGATGGCCCTGAAGGTGATGACACACATGTTTCAACTAGAGTTATGGGTACTCAAGGTTACGCCGCACCCGAATATATCATGACAGGACATTTGACAGCAATGAGTGATGTGTATAGTTTTGGAGTTGTCCTATTGGAGCTACTAACCGGAAGAAGATCAGTGGACAAGGCACGTCCACCACGAGAACACAATCTAGTGGAATGGGCCAGACCGGTTTTAAACGACGCTCGTAAGCTCACCAGAATAATGGATCCAAGGCTTGAAGGACAGTATTCAGAAATGGGAGCAAGAAAAGCAGCTGCATTAGCTTATCAATGTCTTAGCCATAGACCAAGAAACCGACCTACAATGTCTACCGTAGTGAACACTCTTGAACCTCTTCAAGATTTCGACGATATACCAATAGGTCCATTTGTTTACACAGCTCCGAGTGATGTTAGTAATAATGAAGTGAGGAACGAGAAAACAACATCGATACACCAAAGGAGAGAAAAAGAGAAAGTACTCATCATCATCATCGTAGAAATCAAAATGGTAGTAATAATAATAATGAAGTAGTAGTACATAAGAAAGATGGTGAAGAATATTATGATAATCCTAAAGAGAAGAAAAGGGAAAGTAGTGAGAATCATAGAAGACATCATCATCATCGTCAGAATGGACATAGGCATCCATTAAAGTCACCAAAAGAACAAAGACATGTACATCAAAGTGGGTCACACTCACCTGATACATCCATTACATCAGAAAGCCAAGGAAATTAAGGAACTATAGTTTAGAAGACACGTACCTAGATCAAACACTGCTAAATGTATATATATTGTGTCAATGTCAATGAGCTGGTTTCAAACAAATAAAAGTTGAAAACAAAGAAAAAGGGAGAGGGAAGTGATAATGCAATTATGCAATGGTCCTAGCATAGAAAGCATGCTTGTANTTTTTATAATACTTATTTTTGTATTTCTNGAGGTAAGTTGATTTATTTTAGAATTTGTGTAATACAAATTATTTTAAAATTTAAA
TARGET=1448,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4156
PRIMER_LEFT_PENALTY=0.108684
PRIMER_RIGHT_PENALTY=0.306868
PRIMER_LEFT_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_SEQUENCE=AATGGATGCCTATGTCCATTCT
PRIMER_LEFT=1255,22
PRIMER_RIGHT=1484,22
PRIMER_LEFT_TM=59.891
PRIMER_RIGHT_TM=59.693
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.5040
PRIMER_LEFT_1_PENALTY=0.197140
PRIMER_RIGHT_1_PENALTY=0.306868
PRIMER_LEFT_1_SEQUENCE=TGTTTACACAGCTCCGAGTGAT
PRIMER_RIGHT_1_SEQUENCE=AATGGATGCCTATGTCCATTCT
PRIMER_LEFT_1=1222,22
PRIMER_RIGHT_1=1484,22
PRIMER_LEFT_1_TM=59.803
PRIMER_RIGHT_1_TM=59.693
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.5750
PRIMER_LEFT_2_PENALTY=0.108684
PRIMER_RIGHT_2_PENALTY=0.466342
PRIMER_LEFT_2_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_2_SEQUENCE=GTGAGTGTGACCCACTTTGATG
PRIMER_LEFT_2=1255,22
PRIMER_RIGHT_2=1534,22
PRIMER_LEFT_2_TM=59.891
PRIMER_RIGHT_2_TM=60.466
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.5750
PRIMER_LEFT_3_PENALTY=0.108684
PRIMER_RIGHT_3_PENALTY=0.466342
PRIMER_LEFT_3_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_3_SEQUENCE=TGAGTGTGACCCACTTTGATGT
PRIMER_LEFT_3=1255,22
PRIMER_RIGHT_3=1533,22
PRIMER_LEFT_3_TM=59.891
PRIMER_RIGHT_3_TM=60.466
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.6347
PRIMER_LEFT_4_PENALTY=0.108684
PRIMER_RIGHT_4_PENALTY=0.526059
PRIMER_LEFT_4_SEQUENCE=TGAAGTGAGGAACGAGAAAACA
PRIMER_RIGHT_4_SEQUENCE=TGGTGACTTTAATGGATGCCTA
PRIMER_LEFT_4=1255,22
PRIMER_RIGHT_4=1494,22
PRIMER_LEFT_4_TM=59.891
PRIMER_RIGHT_4_TM=59.474
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_05327 Ps_contig_mira-and-tgicl-ass_franssen_37170
SEQUENCE=CGGGAAATAACATAGATAGAAAAGTACATAGAATTTAACAAAACACAAAACAGAGAAAAATCTAAACAGTCACAACAAGTTCATGATCAGAGACAATTTTACCTCGTAATGATGGAAGAAGAAGAAGAAAAGGGTGACCTAGGTAAAGATAATGATGTAACAAATGTTGAATTTGGTGTTTCAATTTCAGACCCAAAAGGGAAAAATGTTGTTATGAATTCTGAAACTCAGAACAATGGAATTGAAGATGACTGGGATGTGGAAGTGGATATAATTGGGAATTCTGGTTTCAAGGGTGTGGAAAATAGTTGTATGGATTTGGCTGTTGTTGAGAGTTCGAGCTCCAGTTCTTATGGCGACGCGTGTCCTGGTTCTGCATTAACTGATTCTGATGAGGCGGAATCGTCGATTCGTGATGAACGGAGTAAGGGTTTGCCTCGATTGAGAAAGAGAAAAATGACTGATCATTGGAAGAAGTTCATCTTTCCTATAAAGCAGCGATGTAAATGGCTAGAGATTCAACTGAGGAAACTTGACTCCCAAGCAAAGAAATACGAAGARGCAATTGCGG
TARGET=115,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05328 Ps_contig_mira-and-tgicl-ass_franssen_37173
SEQUENCE=TTACATAATCCATCCTCAAACAGTCTTTCTATTATAGTTAAATTTTTTGTCAATGTTCCAATAAATGTACACATCACATAATGTTACCGGGGAGCTTTGTCCATAACTAACATTTAACCAGCAACTGCCACTAGAGAAGGCAAGTCTTCCACAGCCAACCTGGGCAGCCAGCACAGGATCACTCAGTTGCACAAGATTCAGTTCCTAATTTGAAAATTCAAATACACACACACTGTTGCCCTATAAGCTTGGACATACTGTTCAACTACTTGCTGTACACATTGTTCTTGCAAATGCATGGGAATTGAAGTACTCTAACAGATGTTTTCGGCCAAATATATAAAGCCAAAACTAGCACTCAAACTCTACACGGCGGGTTTCAGTTTGCACGGAACTTTATGTCATGGTAACATAGGGTCTCCAGACGACGCGAGCATTTGCAAGCCTGTTTCATCAGCTGTCCGTTTGGAAGCATAATCTTGCATGGACTCACTCAAAGACTGGCGAAGGCCTTCTTTTACAGCATCGAAGTTTTTGTAAGTATAACCAACAAAGTTTAGATCTTTGGGAGTTAGAAGCATCTTTCTTGAAGATCCGGACCCGGCTCTTGTTGCATCTGGTGGTTCTACCTCATCAAACTTCATGTAAGGTTTTGGGTATCCACGTTCCCCATTTAACTTGTTAGGTTTAAATGCTGCATCCTATTTACAATTAAAGTCTACTA
TARGET=224,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0980
PRIMER_LEFT_PENALTY=0.058853
PRIMER_RIGHT_PENALTY=0.039178
PRIMER_LEFT_SEQUENCE=TTAACCAGCAACTGCCACTAGA
PRIMER_RIGHT_SEQUENCE=TGACATAAAGTTCCGTGCAAAC
PRIMER_LEFT=113,22
PRIMER_RIGHT=403,22
PRIMER_LEFT_TM=59.941
PRIMER_RIGHT_TM=60.039
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.1140
PRIMER_LEFT_1_PENALTY=0.074807
PRIMER_RIGHT_1_PENALTY=0.039178
PRIMER_LEFT_1_SEQUENCE=AACTGCCACTAGAGAAGGCAAG
PRIMER_RIGHT_1_SEQUENCE=TGACATAAAGTTCCGTGCAAAC
PRIMER_LEFT_1=122,22
PRIMER_RIGHT_1=403,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=60.039
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=0.1672
PRIMER_LEFT_2_PENALTY=0.052729
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=AGAGAAGGCAAGTCTTCCACAG
PRIMER_RIGHT_2_SEQUENCE=CGTCTGGAGACCCTATGTTACC
PRIMER_LEFT_2=132,22
PRIMER_RIGHT_2=426,22
PRIMER_LEFT_2_TM=60.053
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.2004
PRIMER_LEFT_3_PENALTY=0.085951
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=AGGATCACTCAGTTGCACAAGA
PRIMER_RIGHT_3_SEQUENCE=CGTCTGGAGACCCTATGTTACC
PRIMER_LEFT_3=174,22
PRIMER_RIGHT_3=426,22
PRIMER_LEFT_3_TM=59.914
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=253
PRIMER_PAIR_PENALTY_4=0.2183
PRIMER_LEFT_4_PENALTY=0.052729
PRIMER_RIGHT_4_PENALTY=0.165541
PRIMER_LEFT_4_SEQUENCE=AGAGAAGGCAAGTCTTCCACAG
PRIMER_RIGHT_4_SEQUENCE=CATAAAGTTCCGTGCAAACTGA
PRIMER_LEFT_4=132,22
PRIMER_RIGHT_4=400,22
PRIMER_LEFT_4_TM=60.053
PRIMER_RIGHT_4_TM=60.166
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_05329 Ps_contig_mira-and-tgicl-ass_franssen_37174
SEQUENCE=TACCCGGCCGGGAGGGGGTTGGGCGTGAAAGGTGGGGAAGGAAATGGCAACACGTGGAGGTGAACCGGAGACAAGAATACGAGAGACTACAAAGAAGATTTGATTGGGAGAAGATTCGGTTTATAATCAAGATGCCATTGAGTTGTCTGGTGGAAGATGAAAAGATGCATAGATTTGAACCAATTATCTTTTTTTATAGGTTAGCTTGAATGAATGAATAGAGATGAGAATTTGATTGCATCTCAAGTGGTATCAAACGCAGAGTTATAGTTTTGATGTCACAACGATGGTTATTGTATTGTTTTCAAGTTTTTTACTTATGGTGGCGTGTCATGCAAGTTTCCTAACAATGGTTGATGTAGATAAAGTTGTTCACAAAGTATGTACCTCAGCTAAAGCTTGAAACTGAGGAACATTATTGGTTTCATGTTCAGTATTTTCATTTATACATCTATGGAGCCTTGTATTATTCAAAAGAAATGAAGAGGGTACTAACTTGACTAANTTA
TARGET=207,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3072
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.304841
PRIMER_LEFT_SEQUENCE=AGGTGAACCGGAGACAAGAATA
PRIMER_RIGHT_SEQUENCE=CTGCGTTTGATACCACTTGAGA
PRIMER_LEFT=57,22
PRIMER_RIGHT=261,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.305
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.3072
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.304841
PRIMER_LEFT_1_SEQUENCE=AGGTGAACCGGAGACAAGAATA
PRIMER_RIGHT_1_SEQUENCE=TCTGCGTTTGATACCACTTGAG
PRIMER_LEFT_1=57,22
PRIMER_RIGHT_1=262,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.305
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=0.3072
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.304841
PRIMER_LEFT_2_SEQUENCE=AGGTGAACCGGAGACAAGAATA
PRIMER_RIGHT_2_SEQUENCE=CTCTGCGTTTGATACCACTTGA
PRIMER_LEFT_2=57,22
PRIMER_RIGHT_2=263,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=60.305
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.3651
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.362762
PRIMER_LEFT_3_SEQUENCE=AGGTGAACCGGAGACAAGAATA
PRIMER_RIGHT_3_SEQUENCE=GCGTTTGATACCACTTGAGATG
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=59.637
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.3651
PRIMER_LEFT_4_PENALTY=0.002310
PRIMER_RIGHT_4_PENALTY=0.362762
PRIMER_LEFT_4_SEQUENCE=AGGTGAACCGGAGACAAGAATA
PRIMER_RIGHT_4_SEQUENCE=CGTTTGATACCACTTGAGATGC
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=258,22
PRIMER_LEFT_4_TM=59.998
PRIMER_RIGHT_4_TM=59.637
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_05330 Ps_contig_mira-and-tgicl-ass_franssen_37175
SEQUENCE=CCTTTTAGTCTTTCCTTCCTCCGAAGTTTTTTTTCTCCCTTTCCTTTGTTCTTCTCCTCTCTTTACGTTCTTCTTCTTCACGTTTTTCTTTTTCTTCTTCTTCAAGAAGTTTCATCTGCTTTTCTAATGTNATGATTTCTTTGCATGCTACATGCACTCGTTCCTCGAGCAATTTTAGTGCAAGACAGACAAATATGCTGTGTGCATTTTGGCGAGCTGTTCCTTCTCTGAAAGCTTTTTCAACCTGCTCTTTAAAAATAACAGTAGCAACATCTAGCAGAAATTCTCGAGCCAGTTCAGGGCTTTTTGCGTGCTTTTGGGGACGAGAGCATTCTCCATCAAGATCATTTCCATCCTTATCAACTGAATCATCATCCTCTTCCTCTTCAGCCTCTTCAGCATGCTACAAAAGAATTCTTCGGAATTACCTTTTTCCC
TARGET=68,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3231
PRIMER_LEFT_PENALTY=0.102004
PRIMER_RIGHT_PENALTY=0.221115
PRIMER_LEFT_SEQUENCE=CCCTTTCCTTTGTTCTTCTCCT
PRIMER_RIGHT_SEQUENCE=GCTTTCAGAGAAGGAACAGCTC
PRIMER_LEFT=36,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=59.779
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.3482
PRIMER_LEFT_1_PENALTY=0.101683
PRIMER_RIGHT_1_PENALTY=0.246536
PRIMER_LEFT_1_SEQUENCE=CTTTCCTTCCTCCGAAGTTTTT
PRIMER_RIGHT_1_SEQUENCE=GCATGTAGCATGCAAAGAAATC
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=154,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=59.753
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.3485
PRIMER_LEFT_2_PENALTY=0.102004
PRIMER_RIGHT_2_PENALTY=0.246536
PRIMER_LEFT_2_SEQUENCE=CCCTTTCCTTTGTTCTTCTCCT
PRIMER_RIGHT_2_SEQUENCE=GCATGTAGCATGCAAAGAAATC
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=59.753
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=1.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=119
PRIMER_PAIR_PENALTY_3=0.3552
PRIMER_LEFT_3_PENALTY=0.108707
PRIMER_RIGHT_3_PENALTY=0.246536
PRIMER_LEFT_3_SEQUENCE=GTCTTTCCTTCCTCCGAAGTTT
PRIMER_RIGHT_3_SEQUENCE=GCATGTAGCATGCAAAGAAATC
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=154,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.753
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=0.3585
PRIMER_LEFT_4_PENALTY=0.102004
PRIMER_RIGHT_4_PENALTY=0.256503
PRIMER_LEFT_4_SEQUENCE=CCCTTTCCTTTGTTCTTCTCCT
PRIMER_RIGHT_4_SEQUENCE=TAAAATTGCTCGAGGAACGAGT
PRIMER_LEFT_4=36,22
PRIMER_RIGHT_4=176,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=60.257
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_05331 Ps_contig_mira-and-tgicl-ass_franssen_37175
SEQUENCE=CCTTTTAGTCTTTCCTTCCTCCGAAGTTTTTTTTCTCCCTTTCCTTTGTTCTTCTCCTCTCTTTACGTTCTTCTTCTTCACGTTTTTCTTTTTCTTCTTCTTCAAGAAGTTTCATCTGCTTTTCTAATGTNATGATTTCTTTGCATGCTACATGCACTCGTTCCTCGAGCAATTTTAGTGCAAGACAGACAAATATGCTGTGTGCATTTTGGCGAGCTGTTCCTTCTCTGAAAGCTTTTTCAACCTGCTCTTTAAAAATAACAGTAGCAACATCTAGCAGAAATTCTCGAGCCAGTTCAGGGCTTTTTGCGTGCTTTTGGGGACGAGAGCATTCTCCATCAAGATCATTTCCATCCTTATCAACTGAATCATCATCCTCTTCCTCTTCAGCCTCTTCAGCATGCTACAAAAGAATTCTTCGGAATTACCTTTTTCCC
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3231
PRIMER_LEFT_PENALTY=0.102004
PRIMER_RIGHT_PENALTY=0.221115
PRIMER_LEFT_SEQUENCE=CCCTTTCCTTTGTTCTTCTCCT
PRIMER_RIGHT_SEQUENCE=GCTTTCAGAGAAGGAACAGCTC
PRIMER_LEFT=36,22
PRIMER_RIGHT=234,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=59.779
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.3482
PRIMER_LEFT_1_PENALTY=0.101683
PRIMER_RIGHT_1_PENALTY=0.246536
PRIMER_LEFT_1_SEQUENCE=CTTTCCTTCCTCCGAAGTTTTT
PRIMER_RIGHT_1_SEQUENCE=GCATGTAGCATGCAAAGAAATC
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=154,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=59.753
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.3485
PRIMER_LEFT_2_PENALTY=0.102004
PRIMER_RIGHT_2_PENALTY=0.246536
PRIMER_LEFT_2_SEQUENCE=CCCTTTCCTTTGTTCTTCTCCT
PRIMER_RIGHT_2_SEQUENCE=GCATGTAGCATGCAAAGAAATC
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=59.753
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=1.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=119
PRIMER_PAIR_PENALTY_3=0.3552
PRIMER_LEFT_3_PENALTY=0.108707
PRIMER_RIGHT_3_PENALTY=0.246536
PRIMER_LEFT_3_SEQUENCE=GTCTTTCCTTCCTCCGAAGTTT
PRIMER_RIGHT_3_SEQUENCE=GCATGTAGCATGCAAAGAAATC
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=154,22
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=59.753
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=0.3585
PRIMER_LEFT_4_PENALTY=0.102004
PRIMER_RIGHT_4_PENALTY=0.256503
PRIMER_LEFT_4_SEQUENCE=CCCTTTCCTTTGTTCTTCTCCT
PRIMER_RIGHT_4_SEQUENCE=TAAAATTGCTCGAGGAACGAGT
PRIMER_LEFT_4=36,22
PRIMER_RIGHT_4=176,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=60.257
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_05332 Ps_contig_mira-and-tgicl-ass_franssen_37180
SEQUENCE=CTAGAAACTAGATAGCTAACCGGTAAGTTTGACTCACACGAAATTCCACCGTCCAAAACCGAAATCGTCACCATGTATGATGAATACAAAATGGATAGAGGTCACAATGAAACCGGTGGATACCAAAGCAATCTCGATCCAAGTCATTATCGCGTACACACAATCACAATAACAACATAATGCATATCACATTGTTACAAACCTTTTTTTAGGAAAAAAAATCACAGTGTAATCGAAATCCAGCCATAACGCGATGAGCTCAAATCAAAAATTGAAACAGAAAGACGAATATATGTGATGTAATAAGATGGACAACCGGAATTAATATTATAGTAGGAAATACAAGTTAAAATATTTTTACCATCGTCGAACATAACATTAGCATATCATCATTACTTGCGTCGAATCGGTATAACCGCGACATCCTAATTGTCATGGAGTTGAAAATCAGAATAGCAACATTAATGTTTTGACAGCATAACCTCTTTCTGCTATCGCAGACCAAACATGAATCGGACCGAAAATTTGTTGGTGAATAGTAGGGATTACGTTTGAGTTTGGTTTTTGTGTAAGAAGAGCGAAGGAAGTTTGTGGCGAAGCAGCGGCGGTGAAAGGGTGTCGGCGTGTTCGCGGTGATGGTGGTCGCCGGCGAAACAGTTTATAAAAAAGTCTGGGTTTGGACGTTGTTGTCGGAAGATTTGGCGGAGGTGTGAATGGATGTTGTGGGGTGTGGTGATGCGCAGCGGATGAAGGTAGTAGATTGTGGGGGTTCGCGGTAATGAGTGATTTTTGGTGTTGGTGGGATGTGTGTGTGACGGTGGTCGTAGGTGGTGGAGGCGGAGGCGACGATTTAGGGAGAAGATGCAGGCGGGGGGG
TARGET=805,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3074
PRIMER_LEFT_PENALTY=0.054021
PRIMER_RIGHT_PENALTY=0.253332
PRIMER_LEFT_SEQUENCE=AGAAGAGCGAAGGAAGTTTGTG
PRIMER_RIGHT_SEQUENCE=CTGCATCTTCTCCCTAAATCGT
PRIMER_LEFT=571,22
PRIMER_RIGHT=866,22
PRIMER_LEFT_TM=60.054
PRIMER_RIGHT_TM=59.747
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=0.6358
PRIMER_LEFT_1_PENALTY=0.054021
PRIMER_RIGHT_1_PENALTY=0.581808
PRIMER_LEFT_1_SEQUENCE=AGAAGAGCGAAGGAAGTTTGTG
PRIMER_RIGHT_1_SEQUENCE=TGCATCTTCTCCCTAAATCGTC
PRIMER_LEFT_1=571,22
PRIMER_RIGHT_1=865,22
PRIMER_LEFT_1_TM=60.054
PRIMER_RIGHT_1_TM=60.582
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=1.3528
PRIMER_LEFT_2_PENALTY=0.054021
PRIMER_RIGHT_2_PENALTY=1.298755
PRIMER_LEFT_2_SEQUENCE=AGAAGAGCGAAGGAAGTTTGTG
PRIMER_RIGHT_2_SEQUENCE=ATCTTCTCCCTAAATCGTCGC
PRIMER_LEFT_2=571,22
PRIMER_RIGHT_2=862,21
PRIMER_LEFT_2_TM=60.054
PRIMER_RIGHT_2_TM=59.701
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=292
PRIMER_PAIR_PENALTY_3=1.3528
PRIMER_LEFT_3_PENALTY=0.054021
PRIMER_RIGHT_3_PENALTY=1.298755
PRIMER_LEFT_3_SEQUENCE=AGAAGAGCGAAGGAAGTTTGTG
PRIMER_RIGHT_3_SEQUENCE=GCCTGCATCTTCTCCCTAAAT
PRIMER_LEFT_3=571,22
PRIMER_RIGHT_3=868,21
PRIMER_LEFT_3_TM=60.054
PRIMER_RIGHT_3_TM=59.701
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=1.3573
PRIMER_LEFT_4_PENALTY=1.103921
PRIMER_RIGHT_4_PENALTY=0.253332
PRIMER_LEFT_4_SEQUENCE=AAAAAGTCTGGGTTTGGACGTT
PRIMER_RIGHT_4_SEQUENCE=CTGCATCTTCTCCCTAAATCGT
PRIMER_LEFT_4=663,22
PRIMER_RIGHT_4=866,22
PRIMER_LEFT_4_TM=61.104
PRIMER_RIGHT_4_TM=59.747
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=204
=
PRIMER_SEQUENCE_ID=SSR_05333 Ps_contig_mira-and-tgicl-ass_franssen_37181
SEQUENCE=TAGTTTGANRAAAGCACTTGGAGGCTCTTAAGGATACCACGACAGTTTCATTGGCTAAAGTCAACAGTGGTTACAAGGAGTTGGACATTGCTATAGTTAGAGCCACAAATCATGTTGAACGCCCAGCTAAGGAAAAACACATTCGAGCTATATTTTCAGCGATCTCAGCTACGAGACCTAGGGCTGATGTTGCCTATTGCATCCATGCTCTTGCCAGACGATTATCAAAGACACATAACTGGGCTGTTGCATTGAAAACTTTAATCGTCATTCACCGNGCTCTGAGGGAGGTGGATCCCACATTTCACGAAGAACTCATTAATTATGGAAGAAGTAGAAGTCATATGCTCAACATGGCTCATTTTAAAGATGACTCCAGTCCAAATGCATGGGATTATTCTGCATGGGTTCGCACTTATGCATTATTTTTGGAGGAACGGTTGGAATGTTTTCGTGTGTTGAAGTATGACATTGAGGCAGATCGTCCTAGGACCAAAGATTTGGATACTGCAGAATTGCTAGAGCACTTGCCAGCTTTACAACAACTTCTCTATCGAGTCGTTAGTTGCCAGCCACAAGGGGCAGCTGTACATAATTTTGTGATTCAGTTGGCTCTCTCGTTGGTTGCTTCAGAAAGCATTAAAGTTTATCAAGCTATTAGTGATGGAACTGTTAATATGATTGATAAGTTTTTCGAGATGCAACGTGAAGATGCTCTGAAAGCTCTTGATATATACAGAAGGGTTGGAATGCAGGCTGAGAGATTGTCAGAGTTTTATGAAATATGCCGCAATCTTGATATTGGACGCGGAGAGAAGTTTATTAAAGTTGAGCAGCCCCCGTCATCGTTTATGCAAGCCATGGAAGACTATGTGAGAGATGCTCCCCAGGGAGTAATAGTTCGCAAGGATCAGTCCATTGACAATAAAATTGCCTCTCCAAAAGAAGTTTTGGCTATTGAATATAAAAAGGAACCAGAAGTGCAGGAGGAACGTTCTCTCTCTCCCCCTCCACAGCCTGAACCTGTGAAAGTGGAAACACCACCAGTACAACCACCACCTGATCTTTTGAATATGGAGGATTCTGTTCCAGCTGCTGCAGAGTTGGATGAGAAGAATGCCTTGGCTTTAGCCATCGTTCCCGTCGCTGATCAACAACCTTCTGCTGTTTTAAATCATGCAAATGGAACCACTGGTTGGGAATTGGCACTTGTTACGGCTCCTAGTTCGAACGAGAGTGCTACAGCAGCTAGCAAATTGGCCGGAGGTTTGGACCTGCTTACACTAGACAGCCTATACGACGACGCACTCAGAAGAAACAACCAAAACGTTAGCTATAATCCATGGGAGCAAGCACCGGCGGGAGGCATGATGCAACCTGCAATGCACGATCCGTTTTTCGCCTCTAATAGAATGGCTGCTCCACCTTCAGTTCAAATGGCAGCTATGTCCAACCAGCAACAGGCTTTCATGTATCAACARCAACAGACAAAATAGAATAGATAGAATGCCTCCTCAACAACCATCTGGAAT
TARGET=996,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05334 Ps_contig_mira-and-tgicl-ass_franssen_37184
SEQUENCE=TGTAGCGTCAATACCTCAATATGTTCTTTGCTCTTCAAGTTGGTATCACTTGCCTCCATGACATCAATGACATTTTGCAGATTCTTGATGAATAATTCTCCCCTTATATTAGGAAACTTCCCAATCTCTCTAACACTTGAACCAATATTTTTCTTGCCTACTACAAAAACATTTAGAGTTTGAAGGTTTTCTAGTTCAACAATTTGCTTCGGCATCTTTATTATGCTTGTCCTATCAATATAAAGATGACGCAAATTAATTAATTTACCAACCTGTTCTGGCTAATTTTGNGAGTATTGAGCACTCAGAATAAAATCAAGGTTTGCAAGT
TARGET=254,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0971
PRIMER_LEFT_PENALTY=0.037936
PRIMER_RIGHT_PENALTY=0.059210
PRIMER_LEFT_SEQUENCE=TATCACTTGCCTCCATGACATC
PRIMER_RIGHT_SEQUENCE=CNCAAAATTAGCCAGAACAGGT
PRIMER_LEFT=43,22
PRIMER_RIGHT=291,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=59.941
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.1674
PRIMER_LEFT_1_PENALTY=0.108206
PRIMER_RIGHT_1_PENALTY=0.059210
PRIMER_LEFT_1_SEQUENCE=AGTTGGTATCACTTGCCTCCAT
PRIMER_RIGHT_1_SEQUENCE=CNCAAAATTAGCCAGAACAGGT
PRIMER_LEFT_1=37,22
PRIMER_RIGHT_1=291,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=59.941
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=0.4985
PRIMER_LEFT_2_PENALTY=0.037936
PRIMER_RIGHT_2_PENALTY=0.460533
PRIMER_LEFT_2_SEQUENCE=TATCACTTGCCTCCATGACATC
PRIMER_RIGHT_2_SEQUENCE=AAAATTAGCCAGAACAGGTTGG
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=59.539
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=0.5247
PRIMER_LEFT_3_PENALTY=0.465503
PRIMER_RIGHT_3_PENALTY=0.059210
PRIMER_LEFT_3_SEQUENCE=TGTTCTTTGCTCTTCAAGTTGG
PRIMER_RIGHT_3_SEQUENCE=CNCAAAATTAGCCAGAACAGGT
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=291,22
PRIMER_LEFT_3_TM=59.534
PRIMER_RIGHT_3_TM=59.941
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=271
PRIMER_PAIR_PENALTY_4=0.5687
PRIMER_LEFT_4_PENALTY=0.108206
PRIMER_RIGHT_4_PENALTY=0.460533
PRIMER_LEFT_4_SEQUENCE=AGTTGGTATCACTTGCCTCCAT
PRIMER_RIGHT_4_SEQUENCE=AAAATTAGCCAGAACAGGTTGG
PRIMER_LEFT_4=37,22
PRIMER_RIGHT_4=288,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=59.539
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_05335 Ps_contig_mira-and-tgicl-ass_franssen_37188
SEQUENCE=AGAGAATTAGAACTTTCTGAAGAGCAAAATCGACATTTTAAAAAGGTCAACGAAGAGGATGATGCAATTGTTGATAGGAAGCTTCAAATACGTTTGAAACATAGGAGGCATCAAAAGAAAAGTAAACTGCGAGAAACAAGCACACCTATCCAGTCGATGGAGAGCAATGTACAGAAGCCTTCATCCGTCGATCAATTTAATCTTGATAGGAAGGAAGGATCTCGAGTTGAAGGGGAAGTTGTGTATGACAATAACAAGGTTGAACATTTGAATATGGAGGCTGATAAAATTAAAGGTTTTGATGCCAGTTTTCATGTTGACAATGAAACGCCTATGGGAGATGATAATTTGTCAGATCCTCCCAAATCTTCACTGGGTGATGTTATTGAACAGAGAGGAACCAAGCGTACCAAATGATGGTGAGCTTGATG
TARGET=208,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1968
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.171245
PRIMER_LEFT_SEQUENCE=ATGTACAGAAGCCTTCATCCGT
PRIMER_RIGHT_SEQUENCE=TTATCATCTCCCATAGGCGTTT
PRIMER_LEFT=166,22
PRIMER_RIGHT=346,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=59.829
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.2092
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.183585
PRIMER_LEFT_1_SEQUENCE=ATGTACAGAAGCCTTCATCCGT
PRIMER_RIGHT_1_SEQUENCE=GTACGCTTGGTTCCTCTCTGTT
PRIMER_LEFT_1=166,22
PRIMER_RIGHT_1=409,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=59.816
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.2686
PRIMER_LEFT_2_PENALTY=0.025566
PRIMER_RIGHT_2_PENALTY=0.243031
PRIMER_LEFT_2_SEQUENCE=ATGTACAGAAGCCTTCATCCGT
PRIMER_RIGHT_2_SEQUENCE=GCTTGGTTCCTCTCTGTTCAAT
PRIMER_LEFT_2=166,22
PRIMER_RIGHT_2=405,22
PRIMER_LEFT_2_TM=60.026
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=0.2703
PRIMER_LEFT_3_PENALTY=0.086698
PRIMER_RIGHT_3_PENALTY=0.183585
PRIMER_LEFT_3_SEQUENCE=GGAGAGCAATGTACAGAAGCCT
PRIMER_RIGHT_3_SEQUENCE=GTACGCTTGGTTCCTCTCTGTT
PRIMER_LEFT_3=158,22
PRIMER_RIGHT_3=409,22
PRIMER_LEFT_3_TM=59.913
PRIMER_RIGHT_3_TM=59.816
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.2729
PRIMER_LEFT_4_PENALTY=0.101680
PRIMER_RIGHT_4_PENALTY=0.171245
PRIMER_LEFT_4_SEQUENCE=ACGTTTGAAACATAGGAGGCAT
PRIMER_RIGHT_4_SEQUENCE=TTATCATCTCCCATAGGCGTTT
PRIMER_LEFT_4=89,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=59.829
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_05336 Ps_contig_mira-and-tgicl-ass_franssen_37193
SEQUENCE=TTTCACTCTCTCTGCGCTCAAACATTTTCTTCTTCCTCTCTACCCTAAAACCTAGCCGCTACCGCCGTAGCAAGAGACGGTGATCCCGGCCGCCGATAGCTTGCATAGGAAAAGAGCACAGCAGTTACCTGGCCATTGCTGGAGCTACTTCATCAGTCTCCAGTGCCAAGTGAAAACTCAAAACCTTGCCTAGAGATATATGGAGAAGATGAAAATAGAAGAGGTTCAGTCAACGACAAAGAAACAGCGTGTTGCTACTCACACACACATAAAAGGCCTAGGTCTTGAGGTAAGTGGAAAACCTCTGCCTTTTGCTTCTGGGTTCGTGGGACAAGCTGAGGCAAGAGAAGCATGTGGCCTTGCGGTTGATATGATACGCCAGAAGAAGATGGCTGGTAAGGCAATCCTTCTCGCCGGGCCACCTGGAACTGGCAAAACTGCATTAGCACTTGGGATATGTCAGGAGCTTGGGACTAAGGTTCCATTCTGTCCAATGGTTGGTTCGGAAGTATACTCAACAGAAGTAAAGAAGACTGAGGTTCTTATGGAAAATTTTCGACGGGCTATCGGTCTACGTATTAAAGAAAATAAGGAAGTATATGAAGGCGAGGTGACTGAGCTTTCTCCTGAAGAAACTGAGAGTATAACAGGAGGTTATGGTAAAAGTATTAGCCATGTTATAATTGGGTTGAAGACAGTGAAAGGAACCAAGCAACTAAAGTTGGACCCCACAATATACGATGCTTTGATTAAGGAAAAGGTAGCTGTTGGGGATGTTATATATATAGAGGCAAATAGTGGGGCTGTGAAAAGGGTTGGCCGAAGTGATGCCTTTGCTACTGAGTTTGACCTTGAAGCAGAGGAGTATGTTCCACTTCCTAAGGGAGAGGTTCACAAGAAAAAGGAAATTGTTCAGGACGTGACCCTACATGATCTGGATGCTGCCAATGCACGGCCTCAGGGGGGGCAAGATATTTTGTCGCTTATGGGCCAGATGATGAAGCCTAGGAAAACAGAAATCACTGACAAGTTGAGACAAGAAATTAATAAGGTTGTCAACCGATATATTGATGAAGGTGTTGCGGAGCTTGTCCCTGGGGTTCTATTTATTGATGAGGTGCATATGCTAGATATGGAGTGTTTTTCCTATCTTAATCGTGCTTTAGAGAGCTCCTTATCTCCAATAGTAATCTTTGCCACAAATAGAGGAATATGCTCTGTAAGAGGGGCTGATATGAGCAGTCCTCATGGCATACCTGTTGACCTATTGGATCGATTGATGATCATTCGAACAGTAACCTATGGTCCAGCTGAAATAATTCAGATTCTAGCTATCCGAGCTCAAGTGGAAGAACTGGTTGTGGATGAGGAAAGTTTAGCTTTCCTTGGGGAGATTGGACAAAAGACATCTTTAAGGCATGCGATTCAGCTTTTATCACCTGCCAGCGTTGTTGCCAAGATAAATGGTCGAGAGAACATTTGCAAGGCGGATCTTGAGGAAATTTGTTCACTTTATTTAGATGCCAAATCATCAGCCAAGCTACTTCAAGAGCAGCAAGACAAATACATATCATAATACTATTTTCAGAATGTTGAAATGATTTGCTAGAATCTGAACTTGTTAGGTTTTTACTCTGGTTCTTGTTCAGAATTTCATTCTTGTATGGAAACAGTTTCCTTCCAGAGGTCATACACGCAATCCTTCAGTCCATTTTGTTAGAACATAGTCTTGTAGAACTGAGGGATGCACTAATGCCTATGTTCTAGTCTTAAGTGAGTTGTACTTGGAAAGGTCGAGACTTTTCTAGAAGTCTATTTATATAAAAATGTTGAGACCCCCAT
TARGET=260,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2690
PRIMER_LEFT_PENALTY=0.041981
PRIMER_RIGHT_PENALTY=0.226971
PRIMER_LEFT_SEQUENCE=CAGTCAACGACAAAGAAACAGC
PRIMER_RIGHT_SEQUENCE=CTTAGTCCCAAGCTCCTGACAT
PRIMER_LEFT=226,22
PRIMER_RIGHT=477,22
PRIMER_LEFT_TM=59.958
PRIMER_RIGHT_TM=59.773
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.2964
PRIMER_LEFT_1_PENALTY=0.041981
PRIMER_RIGHT_1_PENALTY=0.254467
PRIMER_LEFT_1_SEQUENCE=CAGTCAACGACAAAGAAACAGC
PRIMER_RIGHT_1_SEQUENCE=CTTACCAGCCATCTTCTTCTGG
PRIMER_LEFT_1=226,22
PRIMER_RIGHT_1=399,22
PRIMER_LEFT_1_TM=59.958
PRIMER_RIGHT_1_TM=60.254
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=174
PRIMER_PAIR_PENALTY_2=0.3456
PRIMER_LEFT_2_PENALTY=0.317850
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=CAGTGCCAAGTGAAAACTCAAA
PRIMER_RIGHT_2_SEQUENCE=ATCCCAAGTGCTAATGCAGTTT
PRIMER_LEFT_2=160,22
PRIMER_RIGHT_2=455,22
PRIMER_LEFT_2_TM=60.318
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=0.3534
PRIMER_LEFT_3_PENALTY=0.325733
PRIMER_RIGHT_3_PENALTY=0.027715
PRIMER_LEFT_3_SEQUENCE=GTGAAAACTCAAAACCTTGCCT
PRIMER_RIGHT_3_SEQUENCE=ATCCCAAGTGCTAATGCAGTTT
PRIMER_LEFT_3=169,22
PRIMER_RIGHT_3=455,22
PRIMER_LEFT_3_TM=59.674
PRIMER_RIGHT_3_TM=60.028
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=0.3534
PRIMER_LEFT_4_PENALTY=0.325733
PRIMER_RIGHT_4_PENALTY=0.027715
PRIMER_LEFT_4_SEQUENCE=GCCAAGTGAAAACTCAAAACCT
PRIMER_RIGHT_4_SEQUENCE=ATCCCAAGTGCTAATGCAGTTT
PRIMER_LEFT_4=164,22
PRIMER_RIGHT_4=455,22
PRIMER_LEFT_4_TM=59.674
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_05337 Ps_contig_mira-and-tgicl-ass_franssen_37193
SEQUENCE=TTTCACTCTCTCTGCGCTCAAACATTTTCTTCTTCCTCTCTACCCTAAAACCTAGCCGCTACCGCCGTAGCAAGAGACGGTGATCCCGGCCGCCGATAGCTTGCATAGGAAAAGAGCACAGCAGTTACCTGGCCATTGCTGGAGCTACTTCATCAGTCTCCAGTGCCAAGTGAAAACTCAAAACCTTGCCTAGAGATATATGGAGAAGATGAAAATAGAAGAGGTTCAGTCAACGACAAAGAAACAGCGTGTTGCTACTCACACACACATAAAAGGCCTAGGTCTTGAGGTAAGTGGAAAACCTCTGCCTTTTGCTTCTGGGTTCGTGGGACAAGCTGAGGCAAGAGAAGCATGTGGCCTTGCGGTTGATATGATACGCCAGAAGAAGATGGCTGGTAAGGCAATCCTTCTCGCCGGGCCACCTGGAACTGGCAAAACTGCATTAGCACTTGGGATATGTCAGGAGCTTGGGACTAAGGTTCCATTCTGTCCAATGGTTGGTTCGGAAGTATACTCAACAGAAGTAAAGAAGACTGAGGTTCTTATGGAAAATTTTCGACGGGCTATCGGTCTACGTATTAAAGAAAATAAGGAAGTATATGAAGGCGAGGTGACTGAGCTTTCTCCTGAAGAAACTGAGAGTATAACAGGAGGTTATGGTAAAAGTATTAGCCATGTTATAATTGGGTTGAAGACAGTGAAAGGAACCAAGCAACTAAAGTTGGACCCCACAATATACGATGCTTTGATTAAGGAAAAGGTAGCTGTTGGGGATGTTATATATATAGAGGCAAATAGTGGGGCTGTGAAAAGGGTTGGCCGAAGTGATGCCTTTGCTACTGAGTTTGACCTTGAAGCAGAGGAGTATGTTCCACTTCCTAAGGGAGAGGTTCACAAGAAAAAGGAAATTGTTCAGGACGTGACCCTACATGATCTGGATGCTGCCAATGCACGGCCTCAGGGGGGGCAAGATATTTTGTCGCTTATGGGCCAGATGATGAAGCCTAGGAAAACAGAAATCACTGACAAGTTGAGACAAGAAATTAATAAGGTTGTCAACCGATATATTGATGAAGGTGTTGCGGAGCTTGTCCCTGGGGTTCTATTTATTGATGAGGTGCATATGCTAGATATGGAGTGTTTTTCCTATCTTAATCGTGCTTTAGAGAGCTCCTTATCTCCAATAGTAATCTTTGCCACAAATAGAGGAATATGCTCTGTAAGAGGGGCTGATATGAGCAGTCCTCATGGCATACCTGTTGACCTATTGGATCGATTGATGATCATTCGAACAGTAACCTATGGTCCAGCTGAAATAATTCAGATTCTAGCTATCCGAGCTCAAGTGGAAGAACTGGTTGTGGATGAGGAAAGTTTAGCTTTCCTTGGGGAGATTGGACAAAAGACATCTTTAAGGCATGCGATTCAGCTTTTATCACCTGCCAGCGTTGTTGCCAAGATAAATGGTCGAGAGAACATTTGCAAGGCGGATCTTGAGGAAATTTGTTCACTTTATTTAGATGCCAAATCATCAGCCAAGCTACTTCAAGAGCAGCAAGACAAATACATATCATAATACTATTTTCAGAATGTTGAAATGATTTGCTAGAATCTGAACTTGTTAGGTTTTTACTCTGGTTCTTGTTCAGAATTTCATTCTTGTATGGAAACAGTTTCCTTCCAGAGGTCATACACGCAATCCTTCAGTCCATTTTGTTAGAACATAGTCTTGTAGAACTGAGGGATGCACTAATGCCTATGTTCTAGTCTTAAGTGAGTTGTACTTGGAAAGGTCGAGACTTTTCTAGAAGTCTATTTATATAAAAATGTTGAGACCCCCAT
TARGET=778,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1159
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.115189
PRIMER_LEFT_SEQUENCE=AAGTTGGACCCCACAATATACG
PRIMER_RIGHT_SEQUENCE=AGATCATGTAGGGTCACGTCCT
PRIMER_LEFT=718,22
PRIMER_RIGHT=935,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.885
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=0.1254
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=AAGTTGGACCCCACAATATACG
PRIMER_RIGHT_1_SEQUENCE=TGGAACATACTCCTCTGCTTCA
PRIMER_LEFT_1=718,22
PRIMER_RIGHT_1=873,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.2175
PRIMER_LEFT_2_PENALTY=0.102276
PRIMER_RIGHT_2_PENALTY=0.115189
PRIMER_LEFT_2_SEQUENCE=GACCCCACAATATACGATGCTT
PRIMER_RIGHT_2_SEQUENCE=AGATCATGTAGGGTCACGTCCT
PRIMER_LEFT_2=724,22
PRIMER_RIGHT_2=935,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=59.885
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.2269
PRIMER_LEFT_3_PENALTY=0.102276
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=GACCCCACAATATACGATGCTT
PRIMER_RIGHT_3_SEQUENCE=TGGAACATACTCCTCTGCTTCA
PRIMER_LEFT_3=724,22
PRIMER_RIGHT_3=873,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=150
PRIMER_PAIR_PENALTY_4=0.2553
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.254567
PRIMER_LEFT_4_SEQUENCE=AAGTTGGACCCCACAATATACG
PRIMER_RIGHT_4_SEQUENCE=CTCTCCCTTAGGAAGTGGAACA
PRIMER_LEFT_4=718,22
PRIMER_RIGHT_4=888,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=59.745
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_05338 Ps_contig_mira-and-tgicl-ass_franssen_37201
SEQUENCE=CTAGTAACCATATAGCATAATCTGAGTTAAATATTCATTAACAGAGATTTATCATTAGCAAGCTTATTTGCTGCGTACAATAAACAAAAATTTGATGTATAGAGATAGTAAGAAAAGAAATAAAAAATTGCCAATTCTAGTACTGAAAATGAAAATCTCCCGTGATAGCAAAACAATTTAAACAAGGATTAACCCTTGTACCAAAATATCTCATTTTATAGACCTAACATGAGAAAAAATGCTATTAAAAAACTCTGGAAGCAAAATAGTGATAATCTTAACTCAAACATGAAGTCTCAAACTTATTTCTTCTTTCCACCCTTGGCAGCTTCAGAAGCCTTGGTCTTCTTAACACCACGGATCTTCTTGGCTCTATTCTTTCTCTCCTTCATTTGCTTCCTTGACTTCTCTACTTTGGTGTCAAGACCATTCCTGATAAGTCTATACTTGGGCTCGTACTTCTTGGCACTCTCAACTGAATCATAAATCAAGCCAAAACCAGTGGATTTTCCACCACCAAAATGTGTTTTGAATTGGAAGACAAACACAGTGTTAGTGTCCTTCACATTATACATAGTTGCCAGCTTTTCCTTAAGCTCAGCCCTAGAAACATTAGGTTTCCCTGGATGATTAGCTTCAACAACGAATTGCTTTCTGGAAAGGAGACGATTGGTGAGGAACTTTCTAGTCCTGATGGTAATAGCTTTCTCTGCCATGTTTTTGAGATGAATGAATGATGCGAGGAATGGTAGTGAAAGAAGTGATCAGAGAGCGCTTCAGACTCAACAAACACATCCCCCCG
TARGET=726,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2465
PRIMER_LEFT_PENALTY=0.234069
PRIMER_RIGHT_PENALTY=0.012470
PRIMER_LEFT_SEQUENCE=CTTTCTGGAAAGGAGACGATTG
PRIMER_RIGHT_SEQUENCE=GGGATGTGTTTGTTGAGTCTGA
PRIMER_LEFT=650,22
PRIMER_RIGHT=797,22
PRIMER_LEFT_TM=60.234
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=0.3086
PRIMER_LEFT_1_PENALTY=0.296177
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=AACATTAGGTTTCCCTGGATGA
PRIMER_RIGHT_1_SEQUENCE=GGGATGTGTTTGTTGAGTCTGA
PRIMER_LEFT_1=608,22
PRIMER_RIGHT_1=797,22
PRIMER_LEFT_1_TM=59.704
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.3302
PRIMER_LEFT_2_PENALTY=0.130883
PRIMER_RIGHT_2_PENALTY=0.199304
PRIMER_LEFT_2_SEQUENCE=TGGATGATTAGCTTCAACAACG
PRIMER_RIGHT_2_SEQUENCE=AGCGCTCTCTGATCACTTCTTT
PRIMER_LEFT_2=623,22
PRIMER_RIGHT_2=775,22
PRIMER_LEFT_2_TM=60.131
PRIMER_RIGHT_2_TM=59.801
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=153
PRIMER_PAIR_PENALTY_3=0.3302
PRIMER_LEFT_3_PENALTY=0.130883
PRIMER_RIGHT_3_PENALTY=0.199304
PRIMER_LEFT_3_SEQUENCE=TGGATGATTAGCTTCAACAACG
PRIMER_RIGHT_3_SEQUENCE=AAGCGCTCTCTGATCACTTCTT
PRIMER_LEFT_3=623,22
PRIMER_RIGHT_3=776,22
PRIMER_LEFT_3_TM=60.131
PRIMER_RIGHT_3_TM=59.801
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.3665
PRIMER_LEFT_4_PENALTY=0.354011
PRIMER_RIGHT_4_PENALTY=0.012470
PRIMER_LEFT_4_SEQUENCE=TTTCCTTAAGCTCAGCCCTAGA
PRIMER_RIGHT_4_SEQUENCE=GGGATGTGTTTGTTGAGTCTGA
PRIMER_LEFT_4=586,22
PRIMER_RIGHT_4=797,22
PRIMER_LEFT_4_TM=59.646
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_05339 Ps_contig_mira-and-tgicl-ass_franssen_37204
SEQUENCE=ACCTTAGCAACTACCTAAAAACAACGTTTTATTGGAAACACATACTTTGATTTTAACGGATCCATCTAACAAAACACGGTACTCTTGTTGTCGGTACAACACTTACTTACCACTGGTAAAATGCTAAGTATTACTGTAAACAGTCTAATATCCGACATTCATGTTCAATAGAAACAAGACTAAATGGCAGTAGCAAGTCTAGTCTAGTTTAAACACTACAGGATAACCATGAAAATAACTCTGGTATAATAATCATCCATTAAACAATTCAAATCAACTCAAACATTGCACCAATATTTTCCAGAAGTGTGCTGGAACTTTGGAGATCACCATTCATATAAATATCAAACATCCCCTGACAGAACCTTGGGCGGTGATCTTGGAGATACTGAACGGGAGGGTGAACGTGATCGTGATCGTGGCCTTGAAGCCTTGATTGGTGATGCTGATCTGGATCTAGAAATAGATCGTGACCTGGACACAGATCGCTCCAAAGATCTACTTTTAGCCCTTTTTGGACTTCTGCTCCTGCTGGGGCTTCTGCTGCGGCTCCTGGAACGACTGCTCTCATACTTCTTCACCCGAATATATGATCTTGCCCAAGGATTTTTAAACTCGGTGTCATCCAGTTTCCGAATAGCGTATTTCATGTCATCATAATTAGTGTAATCAACAAGGCCAAAAGTCCCTTCACTATCACGGGAAACTTCAGCAAAACAAACATCACCAGCTTTTCTCATATGATCCTTCAAATCTTGCCAGGAAGCAGAAGATGGGAGTCCACGAACAATAACTCGAAATTCAGAATGGCGTGAGACACCAAATCGGCCCCCACCAGCACCACCACCACCTCCTCCTGCACCACCTCCGCCACCACTGCCACCATACCCCCGTCGATCACTTGATGATGGCCCTCTACCTCCATGGGCAAGCTCAACCCTTAATCGACAACCATCAAAGTTGTAACCATCCCTACCCCGAATTGCATCTTCTGCATCTCGAGCATTGTCAAACTCAACAAAACAATAACATGGAGGGCGAGGAGGAACTTTCAATTCAATTTCCATTATGCGGCCATACTTGTAAAATAGATCATCAATTTCTGATTCCCTTACATCAGAGGGCAGGTTCCCAACATAGATTGTGCGAGAAAAGCGACTACTCATATCTGATTGGAGAAATTACACAATTCGTCAGAAAGATTACAGCTTGAGTACCCAATCGAGCCTTCGCGAAAGTGAAGCCGGAGCTGGAAACAGATCCTTGCAAATTCGAGAGTGCGAAGAGCCGACCCCGAC
TARGET=839,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1503
PRIMER_LEFT_PENALTY=0.021195
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=GGGAAACTTCAGCAAAACAAAC
PRIMER_RIGHT_SEQUENCE=TTGTCGATTAAGGGTTGAGCTT
PRIMER_LEFT=700,22
PRIMER_RIGHT=950,22
PRIMER_LEFT_TM=60.021
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.2457
PRIMER_LEFT_1_PENALTY=0.116582
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=ATGGGAGTCCACGAACAATAAC
PRIMER_RIGHT_1_SEQUENCE=TTGTCGATTAAGGGTTGAGCTT
PRIMER_LEFT_1=772,22
PRIMER_RIGHT_1=950,22
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=179
PRIMER_PAIR_PENALTY_2=0.2589
PRIMER_LEFT_2_PENALTY=0.129818
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=GGGAGTCCACGAACAATAACTC
PRIMER_RIGHT_2_SEQUENCE=TTGTCGATTAAGGGTTGAGCTT
PRIMER_LEFT_2=774,22
PRIMER_RIGHT_2=950,22
PRIMER_LEFT_2_TM=59.870
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=0.3620
PRIMER_LEFT_3_PENALTY=0.021195
PRIMER_RIGHT_3_PENALTY=0.340760
PRIMER_LEFT_3_SEQUENCE=GGGAAACTTCAGCAAAACAAAC
PRIMER_RIGHT_3_SEQUENCE=GGGGTAGGGATGGTTACAACTT
PRIMER_LEFT_3=700,22
PRIMER_RIGHT_3=978,22
PRIMER_LEFT_3_TM=60.021
PRIMER_RIGHT_3_TM=60.341
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.4128
PRIMER_LEFT_4_PENALTY=0.283676
PRIMER_RIGHT_4_PENALTY=0.129103
PRIMER_LEFT_4_SEQUENCE=CAAACATCACCAGCTTTTCTCA
PRIMER_RIGHT_4_SEQUENCE=TTGTCGATTAAGGGTTGAGCTT
PRIMER_LEFT_4=717,22
PRIMER_RIGHT_4=950,22
PRIMER_LEFT_4_TM=60.284
PRIMER_RIGHT_4_TM=60.129
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=234
=
PRIMER_SEQUENCE_ID=SSR_05340 Ps_contig_mira-and-tgicl-ass_franssen_37218
SEQUENCE=AGTTATGTTTTNAATTACATAATTATTTGATTAGGTTTGAAATAGTGGTTGTTGTTGTTCTAGTTAAATGGATGGAGCGCACAGTTAGATTCCAGTTTGTTGTTCAATGTTCCGGTCATCTCTCAGCTCCACATAGATTCTAAAATGGATTTGCCCAACACTCATCTGCACAATCCCAACAATCTCCAACAATCTCCGCCCAATCATGCTGCAAACGGACATCTCGTCTATGTCCGTAGAAAATCAGAAGGAGAAACACCCAAAAATACAGCTTTTGATAATACAAGGCAACTTTATTGTGAAGAGGAGATCGCTCAACCAAAACCTCAGATAAAGGAGCCTAAGGTTTCTTGTTTCCCAGCATTTGCACCTTCACTTGGGAACGTGTATTGGGAAGAGCGTTATCAACAGTTGCACATGTTTTTAAGGAAATTGGACCAATCAAACCAAGCCGACTACATCCAAATGCTTCATTCTCTGTCTTCCATTCAACTTAGCAGACATGCTGTTGAGTTAGAGAAGAGATCAATTCACCTTTCACTCGAAGAAGCTAAAGAATTACAGCGAGTTGCTGCTTTAAACGTCTTGGGGAAACCAGTGAAGAACTTCAAAGCACCGGTGGATCATGGCGAGTGTTCAGACAAGTTGAAGACATCGACATGACCTTTCTGCCTTTTATGCTGCAGTAAATTCCCTAAGGCAGTGGAAACTTGAGACTGTTTGCTGTGCATAGTTGCTAATTGTTTTTANGCTGTATTTGTTTTTAAT
TARGET=48,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05341 Ps_contig_mira-and-tgicl-ass_franssen_37221
SEQUENCE=AAGGGCACAGTATTGGCCATAACAAAATCTTCCACCAAAAAACACTCATTCTCAGGCCGCCGCCGCCGCCGCCTGCCGTTCGGACTGCAGTGAGCCGCCGATCACAGACCACGAAAAACAGCATTGCCTGTATAAAATAATTTGAACTTCAGAGATGGAGTATGTCAGCCCGGAGGGACTTCGCTTGGATGGCCGCCGACCCATGGAAATGCGACAAATTCGAGCAGAGATTGGCGCTGTATCCAAAGCTGACGGCTCTGCCGTTTTCGAGATGGGTAATACCAAAGTGATTGCTGCTGTTTATGGCCCTAGAGAGGTTCAAAATAGGAGCCAACAACTGAGTGGCAAGG
TARGET=56,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3673
PRIMER_LEFT_PENALTY=0.104238
PRIMER_RIGHT_PENALTY=0.263023
PRIMER_LEFT_SEQUENCE=GCACAGTATTGGCCATAACAAA
PRIMER_RIGHT_SEQUENCE=GGTATTACCCATCTCGAAAACG
PRIMER_LEFT=4,22
PRIMER_RIGHT=282,22
PRIMER_LEFT_TM=59.896
PRIMER_RIGHT_TM=59.737
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.4019
PRIMER_LEFT_1_PENALTY=0.104238
PRIMER_RIGHT_1_PENALTY=0.297699
PRIMER_LEFT_1_SEQUENCE=GCACAGTATTGGCCATAACAAA
PRIMER_RIGHT_1_SEQUENCE=TAAACAGCAGCAATCACTTTGG
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=302,22
PRIMER_LEFT_1_TM=59.896
PRIMER_RIGHT_1_TM=60.298
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=299
PRIMER_PAIR_PENALTY_2=0.7914
PRIMER_LEFT_2_PENALTY=0.104238
PRIMER_RIGHT_2_PENALTY=0.687142
PRIMER_LEFT_2_SEQUENCE=GCACAGTATTGGCCATAACAAA
PRIMER_RIGHT_2_SEQUENCE=GGCTGACATACTCCATCTCTGA
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=169,22
PRIMER_LEFT_2_TM=59.896
PRIMER_RIGHT_2_TM=59.313
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=1.0458
PRIMER_LEFT_3_PENALTY=0.104238
PRIMER_RIGHT_3_PENALTY=0.941586
PRIMER_LEFT_3_SEQUENCE=GCACAGTATTGGCCATAACAAA
PRIMER_RIGHT_3_SEQUENCE=AATGCTGTTTTTCGTGGTCTGT
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=124,22
PRIMER_LEFT_3_TM=59.896
PRIMER_RIGHT_3_TM=60.942
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=121
PRIMER_PAIR_PENALTY_4=1.1424
PRIMER_LEFT_4_PENALTY=0.104238
PRIMER_RIGHT_4_PENALTY=1.038144
PRIMER_LEFT_4_SEQUENCE=GCACAGTATTGGCCATAACAAA
PRIMER_RIGHT_4_SEQUENCE=GGGCTGACATACTCCATCTCTG
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=170,22
PRIMER_LEFT_4_TM=59.896
PRIMER_RIGHT_4_TM=61.038
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_05342 Ps_contig_mira-and-tgicl-ass_franssen_37229
SEQUENCE=AACTATATCTCAACGGTCTGACATTCATAAAAGCAGTATCCGCTTTCAAAAACCTCCCAATTTCCAGAACAACAACCTTCCATTCCTTAAAATCACTATCCTGAAACACTCTCTTACACTCATCAACCAACACTTTAAGCTGATTAACCAACTGCTGCAGCATTTCCCTCCGATTCAAAAGCAAACACACAGTCAAAAACCTAGCTAAGAATCTCAATTGCTTATTCGCAATATTAACATCCTGAAACAAACCGTCTTTGAAATAATCACGAGTGAAAATAGCTTCATAGAAAATGAAAGATTCTGATAAGTAATTAAAATCAGATGTTCGCATGTACTGTCCGAAATAGAGCTGGCCGATTCGAGACGCGATTTCGCCGATTTCCCATCGCTTTAACCCGGCTTCAATTAGCTTCTGACGATTCTCTTGTTGAAATTTCCATAACTGTGTGTAGACTTTGAAGACCTTAGAGAAATATGTATCATGCCTGCTGCGTTGGTAGTAAGGTAAGTCTCTGATTTTGGAGAATTTTTTGTCGGCTTTGTCGACGAGAGTCCAGAAAATCTCGCTTACGGGGATGTTGCTGTTGAGATGTTGTTGTGGTTGCGACATTTTCTTCTTCTTCTTCGTTTTTCTTTCGAGCTCTGAGACTATGGTTTTCTACTGTCCGATCTGAGTCATGGAGCTCTTCTTTTTTCTGGGTTTGGATGAACACTCGTAGTC
TARGET=615,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2145
PRIMER_LEFT_PENALTY=0.080808
PRIMER_RIGHT_PENALTY=0.133741
PRIMER_LEFT_SEQUENCE=GCTGTTGAGATGTTGTTGTGGT
PRIMER_RIGHT_SEQUENCE=CCATGACTCAGATCGGACAGTA
PRIMER_LEFT=583,22
PRIMER_RIGHT=683,22
PRIMER_LEFT_TM=60.081
PRIMER_RIGHT_TM=60.134
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=101
PRIMER_PAIR_PENALTY_1=0.2208
PRIMER_LEFT_1_PENALTY=0.087031
PRIMER_RIGHT_1_PENALTY=0.133741
PRIMER_LEFT_1_SEQUENCE=AGTCCAGAAAATCTCGCTTACG
PRIMER_RIGHT_1_SEQUENCE=CCATGACTCAGATCGGACAGTA
PRIMER_LEFT_1=553,22
PRIMER_RIGHT_1=683,22
PRIMER_LEFT_1_TM=59.913
PRIMER_RIGHT_1_TM=60.134
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.7000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=0.2967
PRIMER_LEFT_2_PENALTY=0.162964
PRIMER_RIGHT_2_PENALTY=0.133741
PRIMER_LEFT_2_SEQUENCE=TGCTGCGTTGGTAGTAAGGTAA
PRIMER_RIGHT_2_SEQUENCE=CCATGACTCAGATCGGACAGTA
PRIMER_LEFT_2=489,22
PRIMER_RIGHT_2=683,22
PRIMER_LEFT_2_TM=59.837
PRIMER_RIGHT_2_TM=60.134
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.3285
PRIMER_LEFT_3_PENALTY=0.194782
PRIMER_RIGHT_3_PENALTY=0.133741
PRIMER_LEFT_3_SEQUENCE=TGTTGCTGTTGAGATGTTGTTG
PRIMER_RIGHT_3_SEQUENCE=CCATGACTCAGATCGGACAGTA
PRIMER_LEFT_3=579,22
PRIMER_RIGHT_3=683,22
PRIMER_LEFT_3_TM=59.805
PRIMER_RIGHT_3_TM=60.134
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.3285
PRIMER_LEFT_4_PENALTY=0.194782
PRIMER_RIGHT_4_PENALTY=0.133741
PRIMER_LEFT_4_SEQUENCE=TTGCTGTTGAGATGTTGTTGTG
PRIMER_RIGHT_4_SEQUENCE=CCATGACTCAGATCGGACAGTA
PRIMER_LEFT_4=581,22
PRIMER_RIGHT_4=683,22
PRIMER_LEFT_4_TM=59.805
PRIMER_RIGHT_4_TM=60.134
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_05343 Ps_contig_mira-and-tgicl-ass_franssen_37240
SEQUENCE=GTCCAAACGGACGATAACAATAAAGGAATCAACCTCAAACTTACTCTCATNTCTTTTCAGTACTGTAACACAAGGCCAGCTGTCATGCCATGGGAGGCTACAATATCGCTAATCTGTCCATTGCAGCATGCATAATTTCACACAACCAGTAGGAAACCTGGCAAATATGTTCGCTTCCTGGATCATATGTAGTAATAACTACCGAAAACTTCTGAGAATACTTTGGCAGCGTAACATACCTGCTTGCATTGTTGTCTTGCCTTTGTTTTTCTTATCAAGCAGATGGTCTGGTGCGACCCTTGTCGTATCTTGGCGGGGTAGGCTTGGCAACATCTACCACTATCATCCAGCCATTAATAATCTTGCCATTCATCTCTTTGAGTGCTGCACTTGCAGCCTCTTCTGTGGTATATTCAATAAATGCATAACCCTTGGATCTTTTTGAAATTTTATCTATGATAACTTTAACTTCAACAAGCTCACCAAAGCCTTCAAATGCAGCACGTAAGGTTTTCTCAGATGTATAAAATGATAGCCCTGTCACAAAAAGTTTCTTTGTTTTCACTGAAGTTTCTTGAGTTCCTTCCAAAGAATTAGGCACATTGAGTCTATTTTCCAAGTTTCTACCTTCATAATTCTTATTTTCAGAGTCAAAATTGTGGTCTGGCTGAACCGACAAAACACCAGGCACACCAGCTAACTCATGTGCACGGTCCTCATCAATTTCACAACAAAAGCCAAAGTTGGTTTTCCAAGAAACATGATATAAAGACATCTGTGCATATTTTTCATTTCCCGTAACCTTAGTCAAAATTTGAGCATAATGATCAACAATCTGAGCTTTGCTAACAACACCAACAGCGGGCTTATCCATTCTGACAATCCAATGCTTTGAATTTCCCGCAGGAAACAACTTATCAGTTTGTGAAATTGATGGTTGGGCATTCGTTGCTGCGATTCTGGTAAAAGAGGAATTACATGAAATGGAAGAAGAAGAAGAAGAANAAAGAAGAAGAAAGAAAGTAAGTTAAGTTAGTTAAGTAAGTAGTAGTAGTTGTTGTTGTAGTAGTAGNAGTARGTAAGTAGTAGTGTAGTAGTGTAGGAGG
TARGET=987,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05344 Ps_contig_mira-and-tgicl-ass_franssen_37240
SEQUENCE=GTCCAAACGGACGATAACAATAAAGGAATCAACCTCAAACTTACTCTCATNTCTTTTCAGTACTGTAACACAAGGCCAGCTGTCATGCCATGGGAGGCTACAATATCGCTAATCTGTCCATTGCAGCATGCATAATTTCACACAACCAGTAGGAAACCTGGCAAATATGTTCGCTTCCTGGATCATATGTAGTAATAACTACCGAAAACTTCTGAGAATACTTTGGCAGCGTAACATACCTGCTTGCATTGTTGTCTTGCCTTTGTTTTTCTTATCAAGCAGATGGTCTGGTGCGACCCTTGTCGTATCTTGGCGGGGTAGGCTTGGCAACATCTACCACTATCATCCAGCCATTAATAATCTTGCCATTCATCTCTTTGAGTGCTGCACTTGCAGCCTCTTCTGTGGTATATTCAATAAATGCATAACCCTTGGATCTTTTTGAAATTTTATCTATGATAACTTTAACTTCAACAAGCTCACCAAAGCCTTCAAATGCAGCACGTAAGGTTTTCTCAGATGTATAAAATGATAGCCCTGTCACAAAAAGTTTCTTTGTTTTCACTGAAGTTTCTTGAGTTCCTTCCAAAGAATTAGGCACATTGAGTCTATTTTCCAAGTTTCTACCTTCATAATTCTTATTTTCAGAGTCAAAATTGTGGTCTGGCTGAACCGACAAAACACCAGGCACACCAGCTAACTCATGTGCACGGTCCTCATCAATTTCACAACAAAAGCCAAAGTTGGTTTTCCAAGAAACATGATATAAAGACATCTGTGCATATTTTTCATTTCCCGTAACCTTAGTCAAAATTTGAGCATAATGATCAACAATCTGAGCTTTGCTAACAACACCAACAGCGGGCTTATCCATTCTGACAATCCAATGCTTTGAATTTCCCGCAGGAAACAACTTATCAGTTTGTGAAATTGATGGTTGGGCATTCGTTGCTGCGATTCTGGTAAAAGAGGAATTACATGAAATGGAAGAAGAAGAAGAAGAANAAAGAAGAAGAAAGAAAGTAAGTTAAGTTAGTTAAGTAAGTAGTAGTAGTTGTTGTTGTAGTAGTAGNAGTARGTAAGTAGTAGTGTAGTAGTGTAGGAGG
TARGET=1044,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05345 Ps_contig_mira-and-tgicl-ass_franssen_37243
SEQUENCE=CCTCNTACAAAAACCAAGATGAAAAAAGTAAAACTTTTTGTATATGAAGAAGAAAATGAAGAAATCAAACTCAATCAATCTCTCTGATCTTGAGACTCTTCAACTTCATTCAACACCAGTTTCAAGAAAAACTACAAAACAAGATGGTTCACCAAATTACATGAAGCCCACAAGTAGTTCACAATCAAAAAAGGAACTTTTTCATGTAAGTCTTAAAAAAACTCAATCTTGTTCTGTTTCTAAGAATCTTGATAGAAAAATTTCAAGTGATTATTCAAAAGCTACATGTAAAAAACCTAGTTTGAGTTTGGTTAGAACATTAACAAAAACTACTAGTTTTAAGGGTTCTAGAAATTTTCCTAGAAGAGCTACTTGTTCTTCAACTTTGAAAGATGCAAACTTTCCTTCATATCTTGAGCTTAATCATGGTGGAACTGAGTTAGAGGGAACTTCAGTTAGGAAGGTTTGTTCATATAGTTATTGTTCTCTTAATGGGCATCATCATCATCACGGTGATTTGCCTCCTTTGAAGACGTTTGTGTCTTCGAGGAGGCGCGTGTTGAAGCGTGTGAAGCTTGAAGCGTTGAGTCCTCGAAGTCGAAGATTGAAGACGACGTGTGAGGTAGAAAAGAGGGACTGTGATGTTGAGCGGAATGTGTTTGATGAAAAACCTGAATGTGATGAAATTGATATGGATTTCTTCATTGAAATCTATGCTAGTGGAAAAAAAGATGAGGAATTGAAAGGAGAAGAAGAAATTGGAAAGGCCGATTTTCTGGACGAGGTTGAGGATTATGAAGATGTCATCGAGTCTACAATTGAAGATGATGGTGTAGAAGCAGATTTTCTGAAAGAGGTTGAGGATCAAGAGAAAAGCGAAGATGCGGACGAAGAAGAACAATCGAGTTGGTCTCATGAAGAAATGAGTATGGAAGATGTTTATAACAATACGGATGATTATTCCGAAGCCGATGATATGAAGTGTGATGACGAACAGTTTTATGGATTTGATCAAGAGCATGATGCTGATTCCTCTGTTTACACAGACGAGGAAAATGACTCGAAAGATGAATCTTTGTCTCAGAGTTCTCATGACATATCAGTGACATGGTTAGACGATATCATTAGCAGCTATTACGATGACATTATTCTGTTTGATGAAACACTCAAAGAACACAAATCTGAAGAAATCATCTATCTTGAAGACGAACCTCTTGATGGCATGATTAGTTTTGTCCTTGACGACAAAATTGGAAGCAACGAGACTCAAGATATCGGTTACCCTACCAGTGACGTCGATTGTGACCAATCTTTGGTAGATGAAATATTTGATTACCTAACAAATTCCGAAGAGAAAGTTGATGAGACTAGCGAAGAGAACAAAGATTTCAAGCGAGGGGAAAAAAACCAAATTCAAACGTCTACGAGTGAATTTGAACTTTGTTCCTTGAAACTAACAATGACAGACCAAACTGTTACCGCTGACGTGATAACTCATAGACACGACAACCATATTCCATTAGTCGATGTTGTTGAAGAAAGCATCAAAGATATTCAAAATCAAGGAAAAAGTAATAAAAGATCAAGTTGCGTAGTCGAGGAAGATGAAAATGCAAGGGAGAATTGGAAAGGTGTGATTAGAAGAAAGATGTGTGTTGACGACGATGATGAGATGAGGAAGTTCAATCCAAGAGATCCGAATTTCCTTCCATTGGTTCCTGAAAAAGAAAAAGAAAAAGTCGATTTGAGGCATCAAATGATGGACGAGCGAAAGAATGCGGAGGATTGGATGGTTGATTGTGCACTAAGACAAGTTGTTAACAAGCTTGCTCCTGCTAGGAAGAAGAAGGTTGCATTGCTAGTTGAAGCTTTTGAAACTGTTATACCTAAATGTGAATCACATTTGAGAAACAATTCAAGGTTTGTTCATGCATGCTAGACACATTCAAGCTTGCATCTGATGAAACCTGCTAAGAATCAAAAATACATGGCACTTGCATGACCAATATGAAGGAGTGACTGATTTTCGTGATCATTTCTGAAGCCTGAAACAATTATGCAGCTTGGAGCTTTTAAGCACTTAAAAGTTGAAACTTCTAAAATGGAAATTGAAGCTCAAGAAAAGGAATCTATGGTTCCAACTTTATGATTATATGGGTTTGCTTTATAATAATTGTTTAGTGTGTGAAACTTTAATTATGTGTTTATTTTGTTCATGTGTGTATCTGATGTACTAATACTTTGTGTGATGAATGTTATCCATAATTGTGTTAATTTATTATTATAATAGTTTGTATTTTGTATCAATCAGTGTATGATATAATGCAAAATAACAACTAATTATTGTTAAAGTTTTAATAAAATT
TARGET=497,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4090
PRIMER_LEFT_PENALTY=0.292414
PRIMER_RIGHT_PENALTY=0.116582
PRIMER_LEFT_SEQUENCE=TCTTGAGCTTAATCATGGTGGA
PRIMER_RIGHT_SEQUENCE=GTCTTCAAAGGAGGCAAATCAC
PRIMER_LEFT=411,22
PRIMER_RIGHT=533,22
PRIMER_LEFT_TM=59.708
PRIMER_RIGHT_TM=60.117
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=0.5008
PRIMER_LEFT_1_PENALTY=0.384229
PRIMER_RIGHT_1_PENALTY=0.116582
PRIMER_LEFT_1_SEQUENCE=GGTGGAACTGAGTTAGAGGGAA
PRIMER_RIGHT_1_SEQUENCE=GTCTTCAAAGGAGGCAAATCAC
PRIMER_LEFT_1=427,22
PRIMER_RIGHT_1=533,22
PRIMER_LEFT_1_TM=59.616
PRIMER_RIGHT_1_TM=60.117
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=107
PRIMER_PAIR_PENALTY_2=0.5913
PRIMER_LEFT_2_PENALTY=0.292414
PRIMER_RIGHT_2_PENALTY=0.298898
PRIMER_LEFT_2_SEQUENCE=TCTTGAGCTTAATCATGGTGGA
PRIMER_RIGHT_2_SEQUENCE=TCACACGTCGTCTTCAATCTTC
PRIMER_LEFT_2=411,22
PRIMER_RIGHT_2=620,22
PRIMER_LEFT_2_TM=59.708
PRIMER_RIGHT_2_TM=60.299
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.6023
PRIMER_LEFT_3_PENALTY=0.292414
PRIMER_RIGHT_3_PENALTY=0.309858
PRIMER_LEFT_3_SEQUENCE=TCTTGAGCTTAATCATGGTGGA
PRIMER_RIGHT_3_SEQUENCE=ACGTCGTCTTCAATCTTCGACT
PRIMER_LEFT_3=411,22
PRIMER_RIGHT_3=616,22
PRIMER_LEFT_3_TM=59.708
PRIMER_RIGHT_3_TM=60.310
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.6103
PRIMER_LEFT_4_PENALTY=0.292414
PRIMER_RIGHT_4_PENALTY=0.317850
PRIMER_LEFT_4_SEQUENCE=TCTTGAGCTTAATCATGGTGGA
PRIMER_RIGHT_4_SEQUENCE=CGAAGACACAAACGTCTTCAAA
PRIMER_LEFT_4=411,22
PRIMER_RIGHT_4=546,22
PRIMER_LEFT_4_TM=59.708
PRIMER_RIGHT_4_TM=60.318
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=136
=
PRIMER_SEQUENCE_ID=SSR_05346 Ps_contig_mira-and-tgicl-ass_franssen_37249
SEQUENCE=CGAAAGAAGTATTGGTTTTGGTAAACAATGGTACGGAAAGTAGGTTAATTTARNTCGTGGGGTGGGCGAAGAATAAAGTTCGCCCCCTACGAGCCCGACGAAGTAGAAATACTGTTGGGCTTCCCTAAGTATCACACAAGAGGTGGTGGAATTAGTAGGACTGACAGATTCAAGTCACTTGGAARTTCTTTCCAGGTTGACACAGTGGCATATCACCTATCTGTTCTGAAGGAAATGTTTCCTGATGGGATCAATGTTCTCTCTCTCTTTTCTGGAATTGGTGGTGCAGAGGTAGCTCTCCATAAACTTGGTGTCCTTCTAAAGAATGTAGTGTCCGTTGAAAAATCTCAAGTGAATAGGGACATTGTTAGAAGTTGGTGGGAGCAAACTAATCAAAAAGGTAATTTGATTGATATGGATGATGTGCAACAACTAGATGCTGATCGTTTGGAGCAGCTGATGAGCACTATTTGGTGTCTTTTGATCTCATTTGGTTGGGTGGGAGTCCATAGTAATAACCGTGGCGTAGGGAAGACCAAACGAAGG
TARGET=258,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05347 Ps_contig_mira-and-tgicl-ass_franssen_37253
SEQUENCE=TCTTGTTTCATTGTAGTTGGATCAAGATTTTGTTTCCTATTTGGTTTTTTTCACTTTCCAAACTTAGAAAATTTTCGTCTAACACATTAACTGGTGTGCCTGAACTTGAATTTGCATTAAACATTGATTCGTACTCCAACTTAGATATATCTTGTATTACATTATAGCACTCCATAACACTTTCTTTATTTACATAGGAAGAATTGGATATTGTTCCCAAGAAGCATGGATATTGAAAAGGAAACAATTCATGTGAAGCATAAAGAAGGGATGAAGCAGCAACTATAGATGGCTTGAATTCCAAAACCTTTACATCTTTTTGTGACTTGAATATAATTTGAGAAGCTCGATCTTTGAGAACCTTTAATGTTATATTATCATCAAGATTAAACAAATTGGTGAAGTAAGGAATGAAAGAGAATGGAGTAATGGAACGCATTCTCCATTGTAATGCTCCTAACACAAGTGCTTCCATTCTTTTTATCGTTTGTGTCTCAAAAATGAATCCACCATCATCATGATTCAGATGACCCTGAACATCAGTGGTAGAGTACTCTGTTTTCAACATTTTTAGTGCTAGAGAAAAACATGTTACTGCAACAAGTTTGTTGGCCCATGGTTTTGGCTGCATTATTCCTTGGTTAGCTAGGAAGCGATCCAAATAGTTGATGGCTAGGTAAGTCACAAATGGATCAAAGGTGCATGACAACTGTGAGATTAAAGAGATGAAATCAGTTCTAAGTGAGGCATCAAACTCGTTTGATTTGAGGGTTTGGAAGTAAGTTTGTGGAGGGATATGGTCAGATTCAATGAGGAAAAGGGAAGAAACACATTGAGAATTAGGAAGGTCATGAAAGTTTTCAAGAGGGTTTTCAAGGTTAAACTCCATGGATGTGTGTGTTTGTAGAGAATTTGTTAGTGATGGTGAGAAGGAAGGAAGATATTAAGCATTGTTAAAGGGTGAGAAAAGAAGTGATAAGGAAAAGGCAATGAGTGAGTTCTTTGAGATGAAAGCTATGGAAGGGGTTGAGTTGTTTTTGCTTTGCTATTACTAGTTGCTAGCTACTCATAAGTATAATGTCTTGGCACGAAACACTATGACTCTATTTTCTTTCCATTTCYCCG
TARGET=929,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05348 Ps_contig_mira-and-tgicl-ass_franssen_37272
SEQUENCE=TTTAATTCAAATTAGCATGTCTATTTTTATTTATTTACATTTTACTACAAATAANAAATTTTGTTGCATCCCACCCCACATAATCATCTACTATGAATCCACCATAACAAATTACTCAAAGAGTTTCCATCATCAAAACCTTTCAAAGTTCTAATATTTCTTCAAAGCATTTTCCTTATCATAAACACCTTGCAAAGCATAAGTAAACCCTTTCCACCCTTCTT
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05349 Ps_contig_mira-and-tgicl-ass_franssen_37284
SEQUENCE=TTTTTTCTGCTTCTTCCTTTTCTTCAGGAGCCATTTTGATTTGCTCTCTTTCTTATTGACCAAATATGAAACTAAAAAATGGCTTACTATTGTTCTTTCTATTTGTGGAGTGTGCTTTTTTCAAAGTGGATTCAAAGTGTGTGAAAGGGTGTGATCTAGCTTTAGCTTCTTACTATGTCATGCCTTTAGTTGAACTCCCAACTATAAAAAACTATATGCAATCAAAGATAGTTACCAACTCTTCTGATGTTTTAAATAGTTACAACAAAGTCTTAGTAACCAATCATGGTAATATTTTTTCCTATTCTAGAATCAACATTCCATTCCCATGTGAATGTATTGGAGGTGAGTTCTTAGGACATGTGTTTGAATATACAACAAAGAAAGGAGATACTTATGATTTGATTGCAAATAATTATTATGTAAGTTTGACAACTGTTGAGCTTTTGAAGAAGTTTAACAGCTATGATCCAAATCATATACCTGCTAAGGCTAAGGTTAATGTTACTGTGAATTGTTCTTGTGGGAATAGCCAGATTTCAAAAGATTATGGCTTGTTTGTTACTTATCCGTTAAGGTCTACGGATTCTCTTGAGAAGATTGCGAACGAGTCGAAACTTGATGAAGGGTTGATACAGAATTTCAACCCTGATGTCAATTTCAGTAGAGGAAGTGGGATAGTGTTCATTCCAGGAAGAGGTATGAATGTTCTTTTCAAAGTATATATCTATGGTATGAATTGTGACGGCTTCGCTTAAAAGCTATTTTTAACTTTGCAGATAAAAATGGAGAATATGTTCCTTTGTATCCTAAGTAAGTAACTTTGATTATCTCACTTCTCATGTTGANATGCTTTATAGATAAAAGTAAATTTGCTTCATATTTGTTTCTAACTTTCATTTTTTTGTTCTTGCAGAACAGGTTTGGCCTTCTGAATTGCTACTAGGATGATTGTTTTTTTTATTGCTTGATGGTTGTTGTGAGGCTAAATTTGGTTATATATATATAAGCAGGTGTTGGTAAGGGTGTAGCTATTGGTATATCTATAGCAGGAGTATTTGCGGTTCTGTTATTTGTTATCTGTATATATGTCAAATACTTCCAGAAAAAGGAAGAAGAGAAAACTATACTGCCCCAAGTTTCTAAGGCGCTTTCGACTCAAGATGGTAATGGTAATTTCTGTGTGAAAATAACTTATGATACAGTTAGGAGTTATTTATTTATTTCAATAAGATTTCGACGATAACTTATGAAAACAACTTTCGGTTTATCGTTAAGCCTCGAGTAGTGGAGAATATGAAACTTCAGGATCTAGTGGGCATGGTACTGGTAGTGCTGCTGGCCTCACAGGAATCATGGTGGCAAAGTCAACTGAGTTTTCATATCAAGAGCTAGCCAAGGCTACAGATAACTTTAGTTTGGATAATAAAATCGGTCAAGGTGGATTTGGAGCTGTCTATTATGCAGAACTCAGAGGCGAGGTACGAAGCTGTTGTGACTCGATCTTTATTTATTGTTAGTGTTCCATATCTGCAACAACATGATATAACTTAGTTTTTATCCTCTCGTACGATTGATTATATGCAGAAAACAGCAATCAAGAAGATGAATGTGCAAGCATCATCAGAATTTCTGTGTGAGTTGAAGGTCTTAACGCATGTTCATCATTTGAATCTGGTATAACAACATGATATAACTACTTAATTAAATGTTTTTCATTGTTAAAATCTTTGTTATGCTTTTTGCATGAACAGGTTCAAATGTGTTATGAAGCATGTTTTAATTTACCTACAATTTTTATGAACAACATAGGTGAGGTTGATTGGATATTGCGTTGAGGGGTCGCTTTTCCTTGTCTATGAACATATTGACAATGGAAACTTGGGTCAATATTTGCATGGTAAAGGTAAGATCAGAAAAAAGAAGTGCTAAGGTTTTGTGATTTTGCAATACTGTCAAACATTTACGAATAATGGCGATATGATTCCAGATAAAGAGCCATTACCATGGTCTAGTAGAGTCCAAATTGCTCTAGATTCAGCACGAGGCCTTGAATACATTCATGAACATACCGTGCCTGTGTATATCCATCGCGATGTAAAATCAGCAAACATATTGATAGACAAAAACTTGCGCGGAAAGGTTACATTATGACCAATCTTAAAAATCTCCTTTCCAAATTTTCATTTCTTTGTTCAGATAAATTAAACTATTGATCATGGTTTTAAATTCAGGTTGCAGATTTTGGCTTGACCAAACTTATTGAAGTTGGAAACTCCACACTTCACACTCGTCTTGTTGGAACTTTTGGATACATGCCACCAGAGTATGACCTATTTTTCCTTATATTATATTCCGATCGCACGTTTTTTGCATGATTAATTAGTAAAGTCGAGTTCTCTTTGTTTTGAAGATATGCTCAATATGGTGACGTTTCTCCGAAAATAGACGTATATGCTTTTGGAGTTGTTCTTTATGAACTGATATCTGCAAAGAATGCTGTTCTGAAGACAGGTGAAGAATCTGTTGCTGAATCAAAGGGTCTTGTAGCCTTGGTAGATATCGATCCCTCTATCAAACTAAACCATTCTTAACACTCAATTCATATACTTTTTCACAATCCAATTGCTATGAATTCACCCTTGACATATTTTTATTGCAGTTTGAAAAAGCACTTAATCAGATTGATCCTTCAGAAGCTCTTCGCAAATTGGTGGATCCTAGGCTTAAAGAAAACTATCCAATTGATTCTGTTTTAAAGATGGCTCAACTTGGGAGAGCATGTACAAGAGATAATCCACTACTACGCCCAAGTATGAGATCTTTAGTTGTTGATCTTATGACACTGTCATCACCATTTGAAGATTGTGATGATGACACTTCCTATGAAAATCAAACTCTCATAAATCTATTGTCAGTTAGATGAAGGTTCTTTTTGCCAGATTGAATCATGTTTGTTAGTAAAACTGAACTAGTTGGGAAGTTTTTTACTTTGTGTTCAAAGTTTATTTCCCAAAATGTTCAAAAGGTCCTAGATTTCAAAAAGACATCCTGTAATTATTTTTAGTGAAGTTGTAACACTGAAGTACAATTTGTATTATGATGTGAAAACTTTATTTTTGCTTTCAAAATGTACATAAGATAAGATTCTAAAC
TARGET=997,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3136
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.276218
PRIMER_LEFT_SEQUENCE=ATTGCTTGATGGTTGTTGTGAG
PRIMER_RIGHT_SEQUENCE=AGAACCGCAAATACTCCTGCTA
PRIMER_LEFT=962,22
PRIMER_RIGHT=1067,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.276
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=0.3812
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.343828
PRIMER_LEFT_1_SEQUENCE=ATTGCTTGATGGTTGTTGTGAG
PRIMER_RIGHT_1_SEQUENCE=GAACCGCAAATACTCCTGCTAT
PRIMER_LEFT_1=962,22
PRIMER_RIGHT_1=1066,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.656
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=0.4122
PRIMER_LEFT_2_PENALTY=0.135999
PRIMER_RIGHT_2_PENALTY=0.276218
PRIMER_LEFT_2_SEQUENCE=GTTTGGCCTTCTGAATTGCTAC
PRIMER_RIGHT_2_SEQUENCE=AGAACCGCAAATACTCCTGCTA
PRIMER_LEFT_2=921,22
PRIMER_RIGHT_2=1067,22
PRIMER_LEFT_2_TM=60.136
PRIMER_RIGHT_2_TM=60.276
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=0.4443
PRIMER_LEFT_3_PENALTY=0.168114
PRIMER_RIGHT_3_PENALTY=0.276218
PRIMER_LEFT_3_SEQUENCE=CTTGATGGTTGTTGTGAGGCTA
PRIMER_RIGHT_3_SEQUENCE=AGAACCGCAAATACTCCTGCTA
PRIMER_LEFT_3=966,22
PRIMER_RIGHT_3=1067,22
PRIMER_LEFT_3_TM=60.168
PRIMER_RIGHT_3_TM=60.276
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=102
PRIMER_PAIR_PENALTY_4=0.4798
PRIMER_LEFT_4_PENALTY=0.135999
PRIMER_RIGHT_4_PENALTY=0.343828
PRIMER_LEFT_4_SEQUENCE=GTTTGGCCTTCTGAATTGCTAC
PRIMER_RIGHT_4_SEQUENCE=GAACCGCAAATACTCCTGCTAT
PRIMER_LEFT_4=921,22
PRIMER_RIGHT_4=1066,22
PRIMER_LEFT_4_TM=60.136
PRIMER_RIGHT_4_TM=59.656
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_05350 Ps_contig_mira-and-tgicl-ass_franssen_37284
SEQUENCE=TTTTTTCTGCTTCTTCCTTTTCTTCAGGAGCCATTTTGATTTGCTCTCTTTCTTATTGACCAAATATGAAACTAAAAAATGGCTTACTATTGTTCTTTCTATTTGTGGAGTGTGCTTTTTTCAAAGTGGATTCAAAGTGTGTGAAAGGGTGTGATCTAGCTTTAGCTTCTTACTATGTCATGCCTTTAGTTGAACTCCCAACTATAAAAAACTATATGCAATCAAAGATAGTTACCAACTCTTCTGATGTTTTAAATAGTTACAACAAAGTCTTAGTAACCAATCATGGTAATATTTTTTCCTATTCTAGAATCAACATTCCATTCCCATGTGAATGTATTGGAGGTGAGTTCTTAGGACATGTGTTTGAATATACAACAAAGAAAGGAGATACTTATGATTTGATTGCAAATAATTATTATGTAAGTTTGACAACTGTTGAGCTTTTGAAGAAGTTTAACAGCTATGATCCAAATCATATACCTGCTAAGGCTAAGGTTAATGTTACTGTGAATTGTTCTTGTGGGAATAGCCAGATTTCAAAAGATTATGGCTTGTTTGTTACTTATCCGTTAAGGTCTACGGATTCTCTTGAGAAGATTGCGAACGAGTCGAAACTTGATGAAGGGTTGATACAGAATTTCAACCCTGATGTCAATTTCAGTAGAGGAAGTGGGATAGTGTTCATTCCAGGAAGAGGTATGAATGTTCTTTTCAAAGTATATATCTATGGTATGAATTGTGACGGCTTCGCTTAAAAGCTATTTTTAACTTTGCAGATAAAAATGGAGAATATGTTCCTTTGTATCCTAAGTAAGTAACTTTGATTATCTCACTTCTCATGTTGANATGCTTTATAGATAAAAGTAAATTTGCTTCATATTTGTTTCTAACTTTCATTTTTTTGTTCTTGCAGAACAGGTTTGGCCTTCTGAATTGCTACTAGGATGATTGTTTTTTTTATTGCTTGATGGTTGTTGTGAGGCTAAATTTGGTTATATATATATAAGCAGGTGTTGGTAAGGGTGTAGCTATTGGTATATCTATAGCAGGAGTATTTGCGGTTCTGTTATTTGTTATCTGTATATATGTCAAATACTTCCAGAAAAAGGAAGAAGAGAAAACTATACTGCCCCAAGTTTCTAAGGCGCTTTCGACTCAAGATGGTAATGGTAATTTCTGTGTGAAAATAACTTATGATACAGTTAGGAGTTATTTATTTATTTCAATAAGATTTCGACGATAACTTATGAAAACAACTTTCGGTTTATCGTTAAGCCTCGAGTAGTGGAGAATATGAAACTTCAGGATCTAGTGGGCATGGTACTGGTAGTGCTGCTGGCCTCACAGGAATCATGGTGGCAAAGTCAACTGAGTTTTCATATCAAGAGCTAGCCAAGGCTACAGATAACTTTAGTTTGGATAATAAAATCGGTCAAGGTGGATTTGGAGCTGTCTATTATGCAGAACTCAGAGGCGAGGTACGAAGCTGTTGTGACTCGATCTTTATTTATTGTTAGTGTTCCATATCTGCAACAACATGATATAACTTAGTTTTTATCCTCTCGTACGATTGATTATATGCAGAAAACAGCAATCAAGAAGATGAATGTGCAAGCATCATCAGAATTTCTGTGTGAGTTGAAGGTCTTAACGCATGTTCATCATTTGAATCTGGTATAACAACATGATATAACTACTTAATTAAATGTTTTTCATTGTTAAAATCTTTGTTATGCTTTTTGCATGAACAGGTTCAAATGTGTTATGAAGCATGTTTTAATTTACCTACAATTTTTATGAACAACATAGGTGAGGTTGATTGGATATTGCGTTGAGGGGTCGCTTTTCCTTGTCTATGAACATATTGACAATGGAAACTTGGGTCAATATTTGCATGGTAAAGGTAAGATCAGAAAAAAGAAGTGCTAAGGTTTTGTGATTTTGCAATACTGTCAAACATTTACGAATAATGGCGATATGATTCCAGATAAAGAGCCATTACCATGGTCTAGTAGAGTCCAAATTGCTCTAGATTCAGCACGAGGCCTTGAATACATTCATGAACATACCGTGCCTGTGTATATCCATCGCGATGTAAAATCAGCAAACATATTGATAGACAAAAACTTGCGCGGAAAGGTTACATTATGACCAATCTTAAAAATCTCCTTTCCAAATTTTCATTTCTTTGTTCAGATAAATTAAACTATTGATCATGGTTTTAAATTCAGGTTGCAGATTTTGGCTTGACCAAACTTATTGAAGTTGGAAACTCCACACTTCACACTCGTCTTGTTGGAACTTTTGGATACATGCCACCAGAGTATGACCTATTTTTCCTTATATTATATTCCGATCGCACGTTTTTTGCATGATTAATTAGTAAAGTCGAGTTCTCTTTGTTTTGAAGATATGCTCAATATGGTGACGTTTCTCCGAAAATAGACGTATATGCTTTTGGAGTTGTTCTTTATGAACTGATATCTGCAAAGAATGCTGTTCTGAAGACAGGTGAAGAATCTGTTGCTGAATCAAAGGGTCTTGTAGCCTTGGTAGATATCGATCCCTCTATCAAACTAAACCATTCTTAACACTCAATTCATATACTTTTTCACAATCCAATTGCTATGAATTCACCCTTGACATATTTTTATTGCAGTTTGAAAAAGCACTTAATCAGATTGATCCTTCAGAAGCTCTTCGCAAATTGGTGGATCCTAGGCTTAAAGAAAACTATCCAATTGATTCTGTTTTAAAGATGGCTCAACTTGGGAGAGCATGTACAAGAGATAATCCACTACTACGCCCAAGTATGAGATCTTTAGTTGTTGATCTTATGACACTGTCATCACCATTTGAAGATTGTGATGATGACACTTCCTATGAAAATCAAACTCTCATAAATCTATTGTCAGTTAGATGAAGGTTCTTTTTGCCAGATTGAATCATGTTTGTTAGTAAAACTGAACTAGTTGGGAAGTTTTTTACTTTGTGTTCAAAGTTTATTTCCCAAAATGTTCAAAAGGTCCTAGATTTCAAAAAGACATCCTGTAATTATTTTTAGTGAAGTTGTAACACTGAAGTACAATTTGTATTATGATGTGAAAACTTTATTTTTGCTTTCAAAATGTACATAAGATAAGATTCTAAAC
TARGET=1213,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4066
PRIMER_LEFT_PENALTY=0.276218
PRIMER_RIGHT_PENALTY=0.130358
PRIMER_LEFT_SEQUENCE=TAGCAGGAGTATTTGCGGTTCT
PRIMER_RIGHT_SEQUENCE=TGCCCACTAGATCCTGAAGTTT
PRIMER_LEFT=1046,22
PRIMER_RIGHT=1320,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=60.130
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.4718
PRIMER_LEFT_1_PENALTY=0.276218
PRIMER_RIGHT_1_PENALTY=0.195574
PRIMER_LEFT_1_SEQUENCE=TAGCAGGAGTATTTGCGGTTCT
PRIMER_RIGHT_1_SEQUENCE=CCACTACTCGAGGCTTAACGAT
PRIMER_LEFT_1=1046,22
PRIMER_RIGHT_1=1290,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=59.804
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.4742
PRIMER_LEFT_2_PENALTY=0.343828
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=ATAGCAGGAGTATTTGCGGTTC
PRIMER_RIGHT_2_SEQUENCE=TGCCCACTAGATCCTGAAGTTT
PRIMER_LEFT_2=1045,22
PRIMER_RIGHT_2=1320,22
PRIMER_LEFT_2_TM=59.656
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.4973
PRIMER_LEFT_3_PENALTY=0.276218
PRIMER_RIGHT_3_PENALTY=0.221115
PRIMER_LEFT_3_SEQUENCE=TAGCAGGAGTATTTGCGGTTCT
PRIMER_RIGHT_3_SEQUENCE=ACCAGTACCATGCCCACTAGAT
PRIMER_LEFT_3=1046,22
PRIMER_RIGHT_3=1330,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.779
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.5394
PRIMER_LEFT_4_PENALTY=0.343828
PRIMER_RIGHT_4_PENALTY=0.195574
PRIMER_LEFT_4_SEQUENCE=ATAGCAGGAGTATTTGCGGTTC
PRIMER_RIGHT_4_SEQUENCE=CCACTACTCGAGGCTTAACGAT
PRIMER_LEFT_4=1045,22
PRIMER_RIGHT_4=1290,22
PRIMER_LEFT_4_TM=59.656
PRIMER_RIGHT_4_TM=59.804
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_05351 Ps_contig_mira-and-tgicl-ass_franssen_37285
SEQUENCE=AGAAGGGAAATTGATTGGAGATTTGAATTTGAGTTTAGAGAGGTAAAACATTTATGCCAAATNTTCCTGTTATGGAGTATTGAGAATAATTCTCTGATAAAATTATAAAATAATTATAAATGAGTTGCACTACTAGAATGCTATATCNNGCAARCCAAYCTTGATGTGCAGTCCAGTAACAATCATCTAGAGATGGGGGCCCACGAAGAAACCTTGATAGAAGCAGCGCTCCGAGTTCTCAACACCGCCGACCCGTTCGAGAAGGCGCGACTCGGTGACTCAGTGGCTTCCCGGTGGCTTGATGGCTCCATCGCCGAACCCTACAAACTCTCTATCGACCTTCTCGTCCCTGATCGCCCAGCAAGGCTATCCAGCGTGAAGTTGGTGGCTCCGGGTCTCATGCCGAAGCTTGGTAAAGCAGGAAGCTTGCAAAGCAGGGTAAACATCGTACATAGCCTTACTCACACCGAAAGCTGGGCTGTTGATCTCTCTTGGGATATAATAGCTCGTTTTGGTAAGCAAGAGGAAATGCCTAGAGAATTCTTTACGGATTTCGTAAAGGTAGCTCAGGATGAAGGGAGACACTTCACTCTTCTTGCTGCACGACTTGAGGAGCTAGGTTCTTATTATGGAGCACTACCAGCTCACGATGGCCTTTGGGATTCTGCCAGTGCCACTTCTAAGGATTTATTATCACGATTAGCAATTGAACATTGCGTCCACGAGGCCAGAGGACTTGATGTGCTGCCTACAACAATCTCACGATTTCGCAATGGTGGCGATAGCACTACAGCAGACTTACTTGAAACTGTAGTTTATCCAGAAGAAATTACACATTGTGCTGCTGGAGTCAAATGGTTCAAGTATCTATGTCAGAGGTCTAGAAATCCAGCCTCAGAGCAAGAAAATTGTGCAGTGGAAAATGAAACAACAGAGGAGAATGAAGTGATTCCAAAGTTTCATTCAATAGTGAGGACATATTTCAGAGGACCTTTAAAACCACCTTTCAATGAGGCAGCAAGAAAAGCGGCTGGATTTGGTCCTGAATGGTATGAACCTCTTGCTGTTAAAGTGCCCAATGCACAATAACCAAACATGTTTCTTTGATTTAATGACTAGTTTCTTGCTGCATATATCTTGTGCAGCCTTACACGGAATATTAGTTTAGGCATTATATATTTGAAATCCTAAATATTGTTTTTTATTTCTTTTTTCAGAATATTTATTTATTAATCGAGATCAAATGATAATGATTTTAGAGAANTAAAGG
TARGET=1220,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05352 Ps_contig_mira-and-tgicl-ass_franssen_37286
SEQUENCE=TTTTCCAGAAGGAAATAAAAAAATGCGCAGTGTTGGGTGAAATCCGAAAGTGCAATAGAAAATAGGGAATTGAGGGAAACATTGTTCAGACAAATCTGTCACAGAAAACAAAACGAAACGAAACGATGATTAGCATTCTCGCCCAGGAACGGTTATTGGGAGCTTCCCTGGGTGTGGTATTGACGAGTGTGGTTGTATTTGAGCAACGCAGATACATCTTCGCTTCGATATCCGATTCCCCATCTCACTCTAAGGTTAGAGAACCCATATTTGGGAAGAAATCACGTTCTGAATTTGCACATTCCTGGAACAAAACTGTGGATCAGACATTTGGACCTTTGATCAAGTCTCTCAGCTCTCGCGGATGGTAGAGATGGTGTGAATTTAATATATTTGTAATACTATCTTTTTGTGATTGTGATATCAGAACACGCAGTAATTTACCTTCACATTCTATATTTGTTTGTCAAACTATAGCAGTGCTCACAAATGTAACGTTTTCTGCAACGCTCGTAAGTTCGTTCAGTATATGCATCAAAATACCTGATTTTGTCTTCTTGTAACT
TARGET=111,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2060
PRIMER_LEFT_PENALTY=0.176730
PRIMER_RIGHT_PENALTY=0.029253
PRIMER_LEFT_SEQUENCE=TAGGGAATTGAGGGAAACATTG
PRIMER_RIGHT_SEQUENCE=TGATCAAAGGTCCAAATGTCTG
PRIMER_LEFT=62,22
PRIMER_RIGHT=344,22
PRIMER_LEFT_TM=60.177
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.2653
PRIMER_LEFT_1_PENALTY=0.176730
PRIMER_RIGHT_1_PENALTY=0.088563
PRIMER_LEFT_1_SEQUENCE=TAGGGAATTGAGGGAAACATTG
PRIMER_RIGHT_1_SEQUENCE=CTTAGAGTGAGATGGGGAATCG
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=253,22
PRIMER_LEFT_1_TM=60.177
PRIMER_RIGHT_1_TM=60.089
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.2659
PRIMER_LEFT_2_PENALTY=0.176730
PRIMER_RIGHT_2_PENALTY=0.089133
PRIMER_LEFT_2_SEQUENCE=TAGGGAATTGAGGGAAACATTG
PRIMER_RIGHT_2_SEQUENCE=AGATGTATCTGCGTTGCTCAAA
PRIMER_LEFT_2=62,22
PRIMER_RIGHT_2=218,22
PRIMER_LEFT_2_TM=60.177
PRIMER_RIGHT_2_TM=59.911
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.3609
PRIMER_LEFT_3_PENALTY=0.176730
PRIMER_RIGHT_3_PENALTY=0.184188
PRIMER_LEFT_3_SEQUENCE=TAGGGAATTGAGGGAAACATTG
PRIMER_RIGHT_3_SEQUENCE=ACCACACTCGTCAATACCACAC
PRIMER_LEFT_3=62,22
PRIMER_RIGHT_3=192,22
PRIMER_LEFT_3_TM=60.177
PRIMER_RIGHT_3_TM=59.816
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=131
PRIMER_PAIR_PENALTY_4=0.4100
PRIMER_LEFT_4_PENALTY=0.176730
PRIMER_RIGHT_4_PENALTY=0.233224
PRIMER_LEFT_4_SEQUENCE=TAGGGAATTGAGGGAAACATTG
PRIMER_RIGHT_4_SEQUENCE=AAGGTCCAAATGTCTGATCCAC
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=338,22
PRIMER_LEFT_4_TM=60.177
PRIMER_RIGHT_4_TM=60.233
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=277
=
PRIMER_SEQUENCE_ID=SSR_05353 Ps_contig_mira-and-tgicl-ass_franssen_37288
SEQUENCE=CAGAATAAACAACAACATTACATTGAAAACAAGATACTGCTTCACATATTACATATTATTGTCTCGGTACATGAATGTCCACACAATATAAATACAATTTTACTTATATAGTCTTAAACATTTCATCTAGCATGTACTAAAATTGATCACAAATTTACAATACTTGATTTCTTCGTAAAGTTCAACCAACCACCACCACCACCACATGATTAATTATCAACTAAGTAAATAACTTAAACATTTTTCTAGCATAATAACTAACTAATTATCTAGTTAACTGTTCTTACTGAAAATATGTTTACCAAGACAACTAGTACATTTTTCTTAAGTTTACTACAAGCTAAACGTATATATATATATACTACTATATTTACACCTCAGAGTTGTGAAAGAATTAGTAAGATTCCACAGAAGCTCTACTGGTAAGAGAAGGTCTCATTATGACAATAGCAGATTCATTAGCACCAGCTTTAAGTGCAGCTTTTCCAGCTTCAAGAAACCTCTTCACTGCATCTTCTGCATACCTGTTTGCTTTTTTTACTGTCATACTTTTCCCGATATTCGGATAGGAGTTGAGTACATGATCGCCGTTGAGCTGAGACGCAAGCTGAATCAGTTCCATTTGGAATTCTGAGAGACTTGAATCTTCTCTAGGTCCATATGGCCTTAGATTAGTGTTGACTTCAGGAAGTGTTTCTGGACAATCATCACGAGGAGTATCTCGAATGTAAAAGTATTTCTCAAAGGTACCAGCCCATGCATCTCTCTTTGTCAAGAAATTGGATTTTAAATTGAAAAGCTTCTTTACTGTGGCAGGAATAGATGAATGTTCATATTGAGAATATGGTGTTGGTCCATCAGGTCCATGAATCACTGTACCCTTATCAATCCAAGGAGAGATCAGAAAAGTGGGAACACGAACACCCAATCTATCAAACCTAAAATAATAAGGGTGAGGCCCAATAATCCCATCAGGGTTAGGAACACCTTCAACCGGTGTAGGAACATGATCATAAAACCCACCATGTTCATCATAAGTTATCAAAATCGCCATTTCTTTCCACTGAGGACTCTTCCTCAATATCTCATACACTTCCTTCACAAACCTCTGTCCTATAGCCACATCATGAGAAGGGTGATCATCGTTCGCCGGAGAAACCTTCACGTCGAAGTAACGCTGCTCAACCACCACATAATTCGGTAACTTCCCCTTCATGGCATGCCTCTTAAACTTCAAACCATAGCTATGAAACTTGACAGTGTTCTTCAACTTTCTCAAACTCTTAGAAAAATGCGTTGCGGGTATTGGTTTTAGGTAAATAGATACCGAAACTACGCATTATCGTGAGCGAGTCGAAGNTTGGTGCGGTTAGG
TARGET=189,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05354 Ps_contig_mira-and-tgicl-ass_franssen_37288
SEQUENCE=CAGAATAAACAACAACATTACATTGAAAACAAGATACTGCTTCACATATTACATATTATTGTCTCGGTACATGAATGTCCACACAATATAAATACAATTTTACTTATATAGTCTTAAACATTTCATCTAGCATGTACTAAAATTGATCACAAATTTACAATACTTGATTTCTTCGTAAAGTTCAACCAACCACCACCACCACCACATGATTAATTATCAACTAAGTAAATAACTTAAACATTTTTCTAGCATAATAACTAACTAATTATCTAGTTAACTGTTCTTACTGAAAATATGTTTACCAAGACAACTAGTACATTTTTCTTAAGTTTACTACAAGCTAAACGTATATATATATATACTACTATATTTACACCTCAGAGTTGTGAAAGAATTAGTAAGATTCCACAGAAGCTCTACTGGTAAGAGAAGGTCTCATTATGACAATAGCAGATTCATTAGCACCAGCTTTAAGTGCAGCTTTTCCAGCTTCAAGAAACCTCTTCACTGCATCTTCTGCATACCTGTTTGCTTTTTTTACTGTCATACTTTTCCCGATATTCGGATAGGAGTTGAGTACATGATCGCCGTTGAGCTGAGACGCAAGCTGAATCAGTTCCATTTGGAATTCTGAGAGACTTGAATCTTCTCTAGGTCCATATGGCCTTAGATTAGTGTTGACTTCAGGAAGTGTTTCTGGACAATCATCACGAGGAGTATCTCGAATGTAAAAGTATTTCTCAAAGGTACCAGCCCATGCATCTCTCTTTGTCAAGAAATTGGATTTTAAATTGAAAAGCTTCTTTACTGTGGCAGGAATAGATGAATGTTCATATTGAGAATATGGTGTTGGTCCATCAGGTCCATGAATCACTGTACCCTTATCAATCCAAGGAGAGATCAGAAAAGTGGGAACACGAACACCCAATCTATCAAACCTAAAATAATAAGGGTGAGGCCCAATAATCCCATCAGGGTTAGGAACACCTTCAACCGGTGTAGGAACATGATCATAAAACCCACCATGTTCATCATAAGTTATCAAAATCGCCATTTCTTTCCACTGAGGACTCTTCCTCAATATCTCATACACTTCCTTCACAAACCTCTGTCCTATAGCCACATCATGAGAAGGGTGATCATCGTTCGCCGGAGAAACCTTCACGTCGAAGTAACGCTGCTCAACCACCACATAATTCGGTAACTTCCCCTTCATGGCATGCCTCTTAAACTTCAAACCATAGCTATGAAACTTGACAGTGTTCTTCAACTTTCTCAAACTCTTAGAAAAATGCGTTGCGGGTATTGGTTTTAGGTAAATAGATACCGAAACTACGCATTATCGTGAGCGAGTCGAAGNTTGGTGCGGTTAGG
TARGET=348,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2471
PRIMER_LEFT_PENALTY=0.826416
PRIMER_RIGHT_PENALTY=0.420685
PRIMER_LEFT_SEQUENCE=CTTCGTAAAGTTCAACCAACCA
PRIMER_RIGHT_SEQUENCE=AGCTGGTGCTAATGAATCTGCT
PRIMER_LEFT=170,22
PRIMER_RIGHT=469,22
PRIMER_LEFT_TM=59.174
PRIMER_RIGHT_TM=60.421
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=1.3030
PRIMER_LEFT_1_PENALTY=0.826416
PRIMER_RIGHT_1_PENALTY=0.476553
PRIMER_LEFT_1_SEQUENCE=CTTCGTAAAGTTCAACCAACCA
PRIMER_RIGHT_1_SEQUENCE=GCTGGTGCTAATGAATCTGCTA
PRIMER_LEFT_1=170,22
PRIMER_RIGHT_1=468,22
PRIMER_LEFT_1_TM=59.174
PRIMER_RIGHT_1_TM=59.523
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=299
PRIMER_PAIR_PENALTY_2=1.3798
PRIMER_LEFT_2_PENALTY=0.959092
PRIMER_RIGHT_2_PENALTY=0.420685
PRIMER_LEFT_2_SEQUENCE=TTCGTAAAGTTCAACCAACCAC
PRIMER_RIGHT_2_SEQUENCE=AGCTGGTGCTAATGAATCTGCT
PRIMER_LEFT_2=171,22
PRIMER_RIGHT_2=469,22
PRIMER_LEFT_2_TM=59.041
PRIMER_RIGHT_2_TM=60.421
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=1.4356
PRIMER_LEFT_3_PENALTY=0.959092
PRIMER_RIGHT_3_PENALTY=0.476553
PRIMER_LEFT_3_SEQUENCE=TTCGTAAAGTTCAACCAACCAC
PRIMER_RIGHT_3_SEQUENCE=GCTGGTGCTAATGAATCTGCTA
PRIMER_LEFT_3=171,22
PRIMER_RIGHT_3=468,22
PRIMER_LEFT_3_TM=59.041
PRIMER_RIGHT_3_TM=59.523
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=1.4436
PRIMER_LEFT_4_PENALTY=1.022866
PRIMER_RIGHT_4_PENALTY=0.420685
PRIMER_LEFT_4_SEQUENCE=TAAAGTTCAACCAACCACCACC
PRIMER_RIGHT_4_SEQUENCE=AGCTGGTGCTAATGAATCTGCT
PRIMER_LEFT_4=175,22
PRIMER_RIGHT_4=469,22
PRIMER_LEFT_4_TM=61.023
PRIMER_RIGHT_4_TM=60.421
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_05355 Ps_contig_mira-and-tgicl-ass_franssen_37291
SEQUENCE=AATTCAAAAACTTCTCGATTCAAACCAAACCGTCATGGAAGCCGAAGCTCTAATCTCCGAGAAAAATTTCGCACTGGAGATTGAAAGCTCTGTCTTTTTCGTTTTTGCTAGGGCTCATATTGCTGAATTAGGGTTTCGTTTCGATAAGGTTGTTGAGGAGAGGGATGAGACCAAGTACGAGGTGGATCTTCAGAACGAGAAGGTGAATGACCTTGTGTTGAGTCTTGAGAACGAGAAGCGGAATGTGGAGAGGATTAGGTTGGAAGCTGGGAGTCTGTTGGAGGAAAAGATTGAGAAAGAAAGAAGGGTTGAAGAGCTTGAAAAGGATAAGGATTTGGTTGTGAAGAAATCAATTGAGTCGGAGAAGGTAATTGATGAGTTGAAGGAGAAGATTGATTTGTTGGTGAAGGAGAAGAATGAGATTGAGAATGTGAATGGTTCTCGGGGGACGAAGATCTTTGACCTTGAACGTGAGCTGCAGCAACACGGTGACCTTTTGAAAAACTCACGGAAAGAAGAGGAGCTGATGCGTGTTAAGATTCTTGAGATGGAGGAAACTATTGGGCTTGCTGTGGACAAGGAGAGGGAAATGAGGGTGGAAAATAGCAATTTGGTTGGAGAGAAGAAGGAGATGGCAAAGAGTATTGAGATTTTAACTGAGGCAAGAGATGGTGTTTTTAGGAAACTTGATGTGGTTCAGAGAGAGTTGGAGAATAGGCAGCACGAGGTTGATGAATCGAATAGAGCGAGAGATGAGATTGAGAAGGTTAAAGTTAGCAGCGAGAATGAAATTATTGAGTTGCGAGGTGAGGTGAATCGACTGAGGGGTGTTGTTGATGAGTTGGAAATGTCTTGCAAAGAGTTTGCAGAGAAAAACAGTGGATTACTGTCTCAAGTTCATCATTATAGGAATGCTATTCAAGAGGTGGAATCTTTGAATGAGCTCAACAGTAAGAAGTTTGAAGAAGAGAAAAACAAAGTAGAGAGTTTTGAGTCACAAGTTGTGGTAATGCAGGAAAAAATTGAGCAATTACTTTCTCAAGTTCAATGTTATAAGAAAGCTGTTGATGAAGTTGTGCTTGAGAGGGATAACATCAAAAAGGGATATGATGAGGAGCTAAACAAAGTAAAGAATTTGGAGTCACAAGTTGTGGCAATGGAGAAAAAGATTGAGCAATTACTTGCTCAAGTTCAATGTTCTAAGAATGCCGTTGATGAAGTGGTGCTTGAGAGGGATAACATCAGAAAGGGATATGATGAAGAGCAAACCAAAGTGGGGAATTTGGAGTTGCGAGTTGCTGAGTTGAAGCTTGAGAAGGATACCGTCAAGAAGGGATATGAAAAGGAGAAGAATAAGGTGGAGAATTTGGAGTTGCAAGTTGCAGGACTGAAGGAAAAAATTGAAAATGCTGAAGCTGATTTGGCGAAGTTAAGAAGCGAGAAAGAGAAGATGAATGAGAGGAACAAGGGGCTGGGTGGCCGTGTAGATGTTTTGATTAAGGAAAAGGATGCTACACATAGTAGCCTTATCGCAGCTCAACGAGAATGCGATGAATTGAGAGCTAAGCTTGACTCATCCTGCATCAACTCAAAGCAGGCCTTGGAATTGTTGAAGAATACTGCTGCTGCAGTTGTGGTTAAGGAAAATGTGGAAGTGATTCCTCGCGGAGGGAAGAAGAAACACGAGAAAGAAGAAATTCAGGCTTTTGCTGAAGAACTGGAGGCGATAAAGAAAGCATTCAAAGTTAAGGAAGAAATGGTGGACGAGATGAAGCAACAACTTGTATCTATGCAGAAATCTGTGTCTGATGCACATAAAGGTAAGCATTTATGGACTGGGATATCCTCTGCTGCTGCAATTTTTGCCGCTGGATTGACTGCCTATTTTGCTAAAGGTCGCTGAAATGTTGTCTTATTTATTTTATAAGATTAGTTTTTGTAATAGGATCAAGATATACCTTGGTTTTTAGCCAAACAATCTTGATCTTTATCATCGAATTAATTACTTTTGTTCT
TARGET=294,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2421
PRIMER_LEFT_PENALTY=0.131699
PRIMER_RIGHT_PENALTY=0.110392
PRIMER_LEFT_SEQUENCE=AGAGGGATGAGACCAAGTACGA
PRIMER_RIGHT_SEQUENCE=CGAGAACCATTCACATTCTCAA
PRIMER_LEFT=158,22
PRIMER_RIGHT=443,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.2421
PRIMER_LEFT_1_PENALTY=0.131699
PRIMER_RIGHT_1_PENALTY=0.110392
PRIMER_LEFT_1_SEQUENCE=GAGGGATGAGACCAAGTACGAG
PRIMER_RIGHT_1_SEQUENCE=CGAGAACCATTCACATTCTCAA
PRIMER_LEFT_1=159,22
PRIMER_RIGHT_1=443,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.2421
PRIMER_LEFT_2_PENALTY=0.131699
PRIMER_RIGHT_2_PENALTY=0.110392
PRIMER_LEFT_2_SEQUENCE=GAGAGGGATGAGACCAAGTACG
PRIMER_RIGHT_2_SEQUENCE=CGAGAACCATTCACATTCTCAA
PRIMER_LEFT_2=157,22
PRIMER_RIGHT_2=443,22
PRIMER_LEFT_2_TM=60.132
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.2837
PRIMER_LEFT_3_PENALTY=0.173354
PRIMER_RIGHT_3_PENALTY=0.110392
PRIMER_LEFT_3_SEQUENCE=ATGTGGAGAGGATTAGGTTGGA
PRIMER_RIGHT_3_SEQUENCE=CGAGAACCATTCACATTCTCAA
PRIMER_LEFT_3=242,22
PRIMER_RIGHT_3=443,22
PRIMER_LEFT_3_TM=59.827
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=202
PRIMER_PAIR_PENALTY_4=0.4205
PRIMER_LEFT_4_PENALTY=0.131699
PRIMER_RIGHT_4_PENALTY=0.288804
PRIMER_LEFT_4_SEQUENCE=AGAGGGATGAGACCAAGTACGA
PRIMER_RIGHT_4_SEQUENCE=TCATTCTTCTCCTTCACCAACA
PRIMER_LEFT_4=158,22
PRIMER_RIGHT_4=419,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=59.711
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_05356 Ps_contig_mira-and-tgicl-ass_franssen_37297
SEQUENCE=AAAACCTTTTGNCGATTCGATAAAGATAGCCTCCCTCAAGATCTCCTCCGCAGGGGAATGGCAGTACCGGACCCAACAAAACCACATGGAATAAGGTTAATAATGAATGATTACCCTTATGCAGAAGATGGTCTTTTAATTTGGTCAGCAATAGAAAATTGGGTTAGAACATATGTGAACTATTACTACCCGAATCCGAGCCTAATAATCAATGACAGAGAGTTACAAGCTTGGTATTCCGAAGCAATCAATGTAGGCCATGCTGATGTAAAGGACGAAACATGGTGGCCGAAACTGAACGACAGCGACAATCTTGTCTCCGTCCTAACAATCTTAATCTGGAACGCGTCCGCGCAACATGCCGCATTGAACTTCGGACAGTACCCTTATGGAGGATACGTGCCAAATCGTCCTCCCTTGATGAGAAGATTGATCCCGGAAGAAAGTGATCCCGAATACGGAAGCTTCATTGCTGATCCGCAAAAGTATTTCCTCAATGCATTGCCTAGTTTGTTGCAGGCTACTAAGTATATGGCCGTTGTCGATACACTTTCAACTCATTCGATCGACGAGGAGTATATAGGCGAGAGACAGCATTCTTCGACTTGGAGCGGTGATGCGGAGATTGTTGAGAAGTTTTACGAATTTTCAGCTGAAATTGGAAGGATTGAGAAGGTGATCGATAGTCGGAATCGTGATAAGACTTTGAGGAACCGTTGCGGCGCGGGTGTTTTGCCTTATGAATTACTTGCTCCTAGTTCTGAACCTGGTGTTACATGCAGAGGTGTCCCAAATAGTGTGTCTACATAATAAAATTTTAAAGTAAATAAATGAGTCACATTGTCACACAGCACATTTTGCTAAGCAATTATGGTTGGTTTATTTATTAGTACTATATGGTAAGTGACTCAGTGGAACACTTGATAATTCTCATTTTGTTCTTAGATTTGATAAATATTATTACTAGTAGACATTGGTGACTTATATATATATCTAGTGTTGTATAGACTATAGTACTTTGGATTGATGAGTTTGGAATTGGAGGGTGTGTGCTTGTTTATAAATTGATTATCACAGTATGTGATTTGAAC
TARGET=983,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5796
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.542262
PRIMER_LEFT_SEQUENCE=TTCTGAACCTGGTGTTACATGC
PRIMER_RIGHT_SEQUENCE=CCAATTCCAAACTCATCAATCC
PRIMER_LEFT=758,22
PRIMER_RIGHT=1041,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.542
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.6153
PRIMER_LEFT_1_PENALTY=0.073088
PRIMER_RIGHT_1_PENALTY=0.542262
PRIMER_LEFT_1_SEQUENCE=CATTGTCACACAGCACATTTTG
PRIMER_RIGHT_1_SEQUENCE=CCAATTCCAAACTCATCAATCC
PRIMER_LEFT_1=838,22
PRIMER_RIGHT_1=1041,22
PRIMER_LEFT_1_TM=60.073
PRIMER_RIGHT_1_TM=60.542
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.6225
PRIMER_LEFT_2_PENALTY=0.080231
PRIMER_RIGHT_2_PENALTY=0.542262
PRIMER_LEFT_2_SEQUENCE=TCACATTGTCACACAGCACATT
PRIMER_RIGHT_2_SEQUENCE=CCAATTCCAAACTCATCAATCC
PRIMER_LEFT_2=835,22
PRIMER_RIGHT_2=1041,22
PRIMER_LEFT_2_TM=60.080
PRIMER_RIGHT_2_TM=60.542
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.6381
PRIMER_LEFT_3_PENALTY=0.095831
PRIMER_RIGHT_3_PENALTY=0.542262
PRIMER_LEFT_3_SEQUENCE=CTGGTGTTACATGCAGAGGTGT
PRIMER_RIGHT_3_SEQUENCE=CCAATTCCAAACTCATCAATCC
PRIMER_LEFT_3=766,22
PRIMER_RIGHT_3=1041,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=60.542
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.6505
PRIMER_LEFT_4_PENALTY=0.108206
PRIMER_RIGHT_4_PENALTY=0.542262
PRIMER_LEFT_4_SEQUENCE=ATGCAGAGGTGTCCCAAATAGT
PRIMER_RIGHT_4_SEQUENCE=CCAATTCCAAACTCATCAATCC
PRIMER_LEFT_4=776,22
PRIMER_RIGHT_4=1041,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=60.542
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_05357 Ps_contig_mira-and-tgicl-ass_franssen_37301
SEQUENCE=ATTACTATTATTCTTTTGATTTCAAGGCTAGTGAATTTAGATATATCTTCTTCTTAATTAATTTCATATGGTTATCATCATCATCATGCTACTTTTTTCTCTTCTCGGGGAATTGAAGCTTTATGGTCCGATTATCTGCTAGATCAAGCAAGTTTATATTGCACCTCTTTCTCTATATTATTTTCTTGGGTTTTTCTGCATATATATAAGTCAATATTATAATATATGGATTGATTATATATTAATAGCGTGGATTGTGTTTTTTTAGATTGGTGTTTTCTATGATAATAAGAGGATGGTTTTTTATTATTTTTTTGTAATTGTGTTGTTGAGAACTACTACTTTTGTGTGTTCCTTTGGTTGTAAATTTGATTTGAAGACTTGTGAAAAAAATATAGATCTGGTTTTCCATATCTATGTAAGATAATAATGTAATTTCTTACGTAAAGAAATTAAAGTTAAGGGTTTTGTATTTACGAACCTAAAAAACC
TARGET=74,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05358 Ps_contig_mira-and-tgicl-ass_franssen_37305
SEQUENCE=GTCGGGGTAANAACTTACCAAAAAAACGGGGGATTGTCTCTCTCATCACTCTCACATTTCTTCACACATTTGTCACACTCTGGAGCCGCGATTCTCTACATCCATGGCTACCGTACAACTCGAATCCTTCTCCCTTATTCCCTCTTCCCAACACCCACGTACAGTCGCTTCCTCCCTCTCCTGCCGCCCGATCGCTGCTAGGTTTCCTCCCTACACCGGTCTCAAGCTACGACCACTCGCCGCTACTAGTTTGCGGAGTCGATTCGCTGCTTCTAGAGTCGTTCCTCGCGGAGGAAGAGTCCTGTGCGAGGCTCGAGATACTGCTGTTGAAGTGGCTTCTATTACTGATGGAAATTGGCAATCCCTTGTAATCGAATCCGACACTCCAGTGTTGGTCGAATTCTGGGCTCCATGGTGCGGTCCCTGCCGAATGATGCATCCCATAATTGACGAGCTGGCAAAGGAATATGTAGGTAAGTTCAAGTGTTACAAACTCAATACTGATGAGAGTCCTTCAACTGCTACTCGCTATGGGATTCGAAGTATTCCAACTGTCATCTTCTTCAAGGATGGTGAAAAGAAAGATGCAATTATCGGTTCTGTACCCAAGGCATCATTGATCACAACTATAGAAAAATTTCTGTGAGGTAACGAACAACAAACAAACAAAAGCTTCAACTGGAAGTAAAGATATTTTTCCCATTAGTCACATCTCATCAAGCCAATTATGTCAAGTTTGAATGGTTTGTTTTCTTAATAATCATGAATGGTTCTGTACAGTTATGGGATGCTTCCCTATTGTTTTTTTAGATGATTTATAGTCACTAATATGACCAATGTTGAAATCTACTAGTATTTTACATCTTTGGCTTCTTTTTTATTCCCACCTTTAACTCAATATTGTTGATATGTAAGAATGGGAATATTT
TARGET=657,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1436
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=0.143555
PRIMER_LEFT_SEQUENCE=CAAGGCATCATTGATCACAACT
PRIMER_RIGHT_SEQUENCE=AGGGAAGCATCCCATAACTGTA
PRIMER_LEFT=606,22
PRIMER_RIGHT=796,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=59.856
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.1436
PRIMER_LEFT_1_PENALTY=0.000037
PRIMER_RIGHT_1_PENALTY=0.143555
PRIMER_LEFT_1_SEQUENCE=CAAGGCATCATTGATCACAACT
PRIMER_RIGHT_1_SEQUENCE=TAGGGAAGCATCCCATAACTGT
PRIMER_LEFT_1=606,22
PRIMER_RIGHT_1=797,22
PRIMER_LEFT_1_TM=60.000
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.1877
PRIMER_LEFT_2_PENALTY=0.044097
PRIMER_RIGHT_2_PENALTY=0.143555
PRIMER_LEFT_2_SEQUENCE=TCGCTATGGGATTCGAAGTATT
PRIMER_RIGHT_2_SEQUENCE=AGGGAAGCATCCCATAACTGTA
PRIMER_LEFT_2=525,22
PRIMER_RIGHT_2=796,22
PRIMER_LEFT_2_TM=59.956
PRIMER_RIGHT_2_TM=59.856
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.1877
PRIMER_LEFT_3_PENALTY=0.044097
PRIMER_RIGHT_3_PENALTY=0.143555
PRIMER_LEFT_3_SEQUENCE=TCGCTATGGGATTCGAAGTATT
PRIMER_RIGHT_3_SEQUENCE=TAGGGAAGCATCCCATAACTGT
PRIMER_LEFT_3=525,22
PRIMER_RIGHT_3=797,22
PRIMER_LEFT_3_TM=59.956
PRIMER_RIGHT_3_TM=59.856
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.2593
PRIMER_LEFT_4_PENALTY=0.115769
PRIMER_RIGHT_4_PENALTY=0.143555
PRIMER_LEFT_4_SEQUENCE=ACTCGCTATGGGATTCGAAGTA
PRIMER_RIGHT_4_SEQUENCE=AGGGAAGCATCCCATAACTGTA
PRIMER_LEFT_4=523,22
PRIMER_RIGHT_4=796,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=59.856
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_05359 Ps_contig_mira-and-tgicl-ass_franssen_37312
SEQUENCE=TAGAGTTCTAAGACCTGGTGGTTATTGGGTTCTTTCTGGTYCTCCAATCAATTGGAAAGTAAACTACAAACCATGGCAGAGACCAAAGGAGGAGCTTGAGGAAGAACAAAGAATGATTGAAGAGGTTGCTAAGAAACTTTGCTGGGAGAAGAAATCTGAGAAGGCTGAAACTGCCATTTGGCAAAAGATGACAGACTCTGAATCATGCCGTAGCAGACAAGATGATTCCAATGTTGAATTTTGTGAATCCTCTGATCCTAATGATGTCTGGTATAAGAAAATGGAGGCCTGTGTTACTCCGACTCCTAAAGTTTCCGGGGGCGATCTTAAACCATTTCCAGACAGGTTATATGCAATCCCTCCGAGAGTTTCTAGCGGCTCTATTCCCGGAGTTTCTTCCGCGACATACCAGGACGATAACAAAACATGGAAAAAGCATGTCAACGCATACAAGAAAATCAACTCACTGTTGGATTCTGGTAGATATCGCAACATCATGGATATGAATGCTGGTTTGGGTAGTTTTGTCTGCAGCTATTCATTCGTCGAAATCATGGGTCATGAATGTGGTGCCAACTATAGCTGAGAAAAGTACTCTCGGTGTGGTATTTGAGCGAGGACTGGTCGGCATCTATCATGATTGGTGTGAAGCCTTTTCCACGTATCCAAGGACATATGATCTCATTCATGCCAATCGCCTCTTTAGTCTCTACCAGGATAAATGCAATACAGAAGACATTCTTCTCGAGATGGACAGGATTTTGCGACCAGAAGGGGCTGTCATAATCCGCGACGAAGTCGATGTATTAATCAAGGTAAAGAAATTAATCGGGGGAATGAGATGGAATTTTAAATTGGTTGATCATGAAGATGGTCCTCTTGTTCCTGAGAAAGTACTAATTGCCGTCAAACAGTATTGGGTTACTGATGGAAACTCCACATCAACACAATAATCACTGGAAAAACAATTTGAAACTACAGTCCTACCCTCAAGCTGATACTACCTTTCTATATACAATGGTCACAGAGTTCATATGGTTTGATTCTTCATCACAACTGTGGCAGAGAGAGAGCTTGGTCCAAGTCAATTTTGTTGTTGAATTAATTTACAGTTTAGAATGTTACATTCAAGAATAGAAACCCTCCTCTCTCTGTTCCTTAATAGTTTCTTTGTTTATTTAAATTATTAATTGTTTTGCTTTGTACCAAAAGTAATTATATTTCTGGATTTGAAGTTGTAGCCAGTCCATTTGTCAATTCTATTGGTGATTATTTTGTAAACAATTTAATGAATTATGTGGCAATCGTGCCTCAATCAACTGTTGGTANAACNNTTAGT
TARGET=1064,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05360 Ps_contig_mira-and-tgicl-ass_franssen_37315
SEQUENCE=CAAACCCTAAAAAAAACACTCTTCTCTTATGTTCCCTTCTTCTGGATCATTCTCTCTTGCAAAATCTTAATCACACCAAAAATGATTTTGGAATAATAAATAATAATAAAACTCAATTTAGAATAAGATTAAACAAACCTCACGTGTGTTAAAATTAAACACATCATCAAATCAAACTACTAGACAAGTAATGTAAGAAGAAGATGAAGATGAAGCAAAAGATGATGATATTCTTCAACCAGTGGTGGTGATGACTTTTGTTATTAATTATCATGTCGATCGTCGTGGCTACCACCGCGGTGATGTGATTGTGATCCACTTGGCTGTTGTTGATGTTGTTGTTGATGAGATTGATGATCGGAAACCGGACGATAAGGACTAAGACCAGCAAGCATACTCAAATGTCCTTCRTTCATGTTCATATTCATATTGATATTTCCACCACCTCCTCCTCCTCCGCCACTACTTCCACCACCACCGCTGACGCCAACGGATGAATTACCTAAGAAAACATGAAAGGAAACGACCTACCAATGAAGACGAGAGGATCCTGTTAGCTCTGGTGATGAGGATGTAAGAATCCCAATGAAACCTTCGTCTTCGCGTTAGGGTGTTCTTCTGAACTCTTTCCTTCCCCGGCGACTCTCAGCGCGGTGAAAAGGTTAGTGATGACCGAAACAGAAACCAAGTTACCGACTCGAACTTTTAGCTCCTCTTCGCGATAATCTTCTGCTTCTCTCCTGTGTGTTGCGTTGTTGTTATTTGGATTGAGTTTGAAGATTGAAGGCTCGATGTGGATCGAAAGTTGGTTGTGGATTTGCAGAGTTTGGTCAGTTTGTGCAAGCAACGAATAGTTCATCCAAGAGGAAGAAGAAGCACTTTTCTCTGCTGCGATTATGATTTTGTTATGCAGGTGGAGGAAAATGGTGGTGGTGAGTTGTGGCTCTCTCGGGAAGAAGAAGGAAGAAAGAAGTTTCCGACGAAGATTCAAGAAGCGGTGTGTTATTGATGGGAGAGGAAGAAGATTCTAGAAGAGGTTGAAGGTTGTGGAAGATGGAGATCTTAATTTCGGTTATGATTTTTCTGAATCCGATGAATGATGATGAGGGAGATATATATAAGGATTGAACCTTCACGGAGTCAAGGTTCCGTTAACATCTCTTAGTGAAGAATCGGTTGAACGTTGGTAACTGTTAAATCCTCTATTTGTTTTGAAGCTAGCTGCGTTTGTGCGTGCGAACTTGGAATGCTGCGTTTTGTTGCATTTGGATTGTTACATTGCGTTTTGGTTTGAACTTATTTCCACTGCATTGTCGTTTACGAGCTGGGCATTTTGGTCTTTGAATTTACCCACTGCGTCGTCGTTTTGGTAGCTTGGCATTTTGAGTTCTGCATTTTTGTACTGTGATTTGTCGTTTCTATTTGCTGAGTTTGCGACTGCCGTTTACTGTTCATGCCGTTTTGGTTTGTTTTTTTATCGTTTTGAGCTGGTTCATGTTGTAACCTGCGTTGGGATTTTTTTGTTGCTTACGTGTACTTGCGAATGGTTCTCTCAATTGCTTCTCTTTTTGTCTCATTCAGCTATTGCACTTGGTGTACTGAACTTTGTAATTCACTTAATTTGAAAATATGGCTATTGGTNATGGTTTGGAACG
TARGET=445,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05361 Ps_contig_mira-and-tgicl-ass_franssen_37318
SEQUENCE=AAGGGAGATCTTGGAAAAGGAAAAGAAGGAAAGAAGCTTAGCATTGTCAGAGAACAAACCTGAACCTGAATTGTGTAGTAGTGCAGACATTCGTCCCTTAAAGATGGAGGATTTTAGATATGCACATGAGCAGGTGTGTGCAAGTGTGTCTTCGGAGTCGACAAATATGAATGAGCTACAACAATGGAATGATCTTTATGGAGAAGGCGGATCTCGAAAAACAAGATCTTTAAGTTACTTCATGTAAAGGTTACTGTAGAGCATTTCTTGCTTAGGCTATTATTATTATTATTATTTCACCTCAAAAAGTGTAACAGGGTCAGCCAGCAGTTGATATGGAAATTCCATATGTGGCTCTTTCTGTAATGTATTATAAGATCTTCCATTTTTCTTTCTCCAACCTCACCTTCTGCTTTACTCCCTCCATTATTTTTTCATACTTAGAAGAGTTTGATAATTTCACTGGGGAACAAAAGTCTTTATTTTAGGCCCTCAGATGATCCAATTACCTTTTCCCCCCTTTGTCTTCTGAATTGTTAAATTACCTTAAGTTTTTTTAACAAATTAAAAAATTAAAAAATTTTAAAAAAAAAATTT
TARGET=278,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0471
PRIMER_LEFT_PENALTY=0.040146
PRIMER_RIGHT_PENALTY=0.006951
PRIMER_LEFT_SEQUENCE=CGGAGTCGACAAATATGAATGA
PRIMER_RIGHT_SEQUENCE=ATTTCCATATCAACTGCTGGCT
PRIMER_LEFT=152,22
PRIMER_RIGHT=342,22
PRIMER_LEFT_TM=59.960
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.0471
PRIMER_LEFT_1_PENALTY=0.040146
PRIMER_RIGHT_1_PENALTY=0.006951
PRIMER_LEFT_1_SEQUENCE=TCGGAGTCGACAAATATGAATG
PRIMER_RIGHT_1_SEQUENCE=ATTTCCATATCAACTGCTGGCT
PRIMER_LEFT_1=151,22
PRIMER_RIGHT_1=342,22
PRIMER_LEFT_1_TM=59.960
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.0474
PRIMER_LEFT_2_PENALTY=0.040447
PRIMER_RIGHT_2_PENALTY=0.006951
PRIMER_LEFT_2_SEQUENCE=CATTCGTCCCTTAAAGATGGAG
PRIMER_RIGHT_2_SEQUENCE=ATTTCCATATCAACTGCTGGCT
PRIMER_LEFT_2=87,22
PRIMER_RIGHT_2=342,22
PRIMER_LEFT_2_TM=59.960
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.1546
PRIMER_LEFT_3_PENALTY=0.040146
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=TCGGAGTCGACAAATATGAATG
PRIMER_RIGHT_3_SEQUENCE=GAGGGAGTAAAGCAGAAGGTGA
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=423,22
PRIMER_LEFT_3_TM=59.960
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.1546
PRIMER_LEFT_4_PENALTY=0.040146
PRIMER_RIGHT_4_PENALTY=0.114470
PRIMER_LEFT_4_SEQUENCE=CGGAGTCGACAAATATGAATGA
PRIMER_RIGHT_4_SEQUENCE=GAGGGAGTAAAGCAGAAGGTGA
PRIMER_LEFT_4=152,22
PRIMER_RIGHT_4=423,22
PRIMER_LEFT_4_TM=59.960
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_05362 Ps_contig_mira-and-tgicl-ass_franssen_37321
SEQUENCE=CGNATAATAAAAACCCTTTCATAGACTATACAAGTATACTATACTATTCCTCTTGTTTATTTTTTTTATTCTTTTTCTTCTTCTTCTCTCAAACTATAGGTGATGCAACAGTAGTTATTATGGATATTAATTCCGGTGAAAATATCATGCCGCCGGCGGTGAACGGCGGCGAGGAAGATGATAAGATGAATATGAATATCAACGGTGGTGGTGGTGGTGGAAATAGGTGGCCCCGCCAAGAAACATTGGCTCTCTTGAAGATAAGATCAGATATGGATGGTGTGTTTCGTGATTCAAGTCTTAAGGGTCCACTTTGGGAAGAAGTTTCAAGGAAACTAGGTGAACTTGGTTATCATAGAAGTTCAAAAAAGTGCAAAGAGAAATTTGAGAATGTATACAAGTACCATAAAAGAACCAAAGAAGGTAGAAGTGGAAAATCAGAAGGAAAAACTTATAGATTTTTTGATGAGTTACAAGCACTTGAGAAAAAAATCACATTCTCTTCTACTTACCCTCCAAAACCACTTCCAAATAATAACACACTCTCATTACCACAAACAACAATGCCTAATGAAACCACAAAAACAATATCACATGTTACAAATATTACTATTCCATCCACAAACCCTACCACACTTATTTCTCCTTCACCACCGCCGCCAACGCCACCACCACAAACAACCAATGCAACAACCGCGACCAACCCTAGTAGCAACAACAATGTTTCATTTTCTTTACCGAACATGAACGTGAACCTATTCTCCACCACAACTACCTCAACTTCTTCTTCCACGGCTTCTGACGAAGACTTGGAAGAGAAGTACAGGAAGAAGAGAAAATGGAAAGACTATTTCAGAAGACTCACAAGAGAAGTGCTTGCGAAACAAGAGGACATGCAAAAGAGATTCTTGGAAGCCATAGACAAACGCGAAAAGGAACACATGGCTCAACAAGATGCTTTGAGAGTTCAAGAAATGGCGAGAATCAATAAAGAACATGAACTTCTTGTCCAAGAAAGATCATCAGCAGCTCAAAAAAACGCCGCGGTTATTACTTTTCTCCAGAAATTGTCCGGACAACCACCACCACAACCGCCGCCACCACCGCCACCACCACCAGCGTCAGAGCTGCCTCGAACTCCGGTATCACAAATACAAACACAGCAACTGGTGATACCAAACAACAACATTGTTGAGATTCTGAGTATGAATAATGGTCACAAAAGTTCTTGCGATGTTGCTTCTCCATCAAGGTGGCCAAAAAGTGAAGTTCATGCTCTAATAAGGATAAGAACAAGTCTAGAACCAAAGTATCAAGAAAATGGTCCAAAAGCACCATTATGGGAAGATATATCAGCTGGAATGAAGAGACTTGGCTACAATAGGAATGCGAAAAGGTGCAAAGAAAAGTGGGAGAATATCAACAAGTACTACAAGAAAATGAAAGAAAGTAACAAACAAAGGCGCGACGATAGTAAGACATGTCCTTATTTTCACGAACTCGAAGCTCTTTACAAAGAAAAGAACAAATTACAGAACCCTTTCGGTTCTAATTCGTTTCATAGTATAAAGTCAAGTGAAATGATGGAGCCATTGATGGTGCAACCAGAACAACAATGGAGACCTCCAACTCAATACGAAGAACCTCACCTGGTGAAGAAGAATAACAACGAAGAGAAAGAAGATGATAACGGTGATGTTGACGATGAAGAAGATGAAAACATGGATGACTACGAAGACGGAGATAGCATGGAAGAAGATGAAGGCGGTGTTAGCAGCCGTTATGAAGTTGTAACAAATAAACTTCCATCAATGAACACCGTTGGATGAGTTGGATGAGTCCATCATCCATCAGTACTGCAGTCTTTTAAGACAGATAGATTCTGAAAGAGAAATTTCTCTATCAGAATCTATCTGTTTCTACGACTGTAGTACACAAGAGAAAAGTTCAATTTGGTAATTTTAAAATTAAAAATTAAAATTTAAGTAAA
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05363 Ps_contig_mira-and-tgicl-ass_franssen_37321
SEQUENCE=CGNATAATAAAAACCCTTTCATAGACTATACAAGTATACTATACTATTCCTCTTGTTTATTTTTTTTATTCTTTTTCTTCTTCTTCTCTCAAACTATAGGTGATGCAACAGTAGTTATTATGGATATTAATTCCGGTGAAAATATCATGCCGCCGGCGGTGAACGGCGGCGAGGAAGATGATAAGATGAATATGAATATCAACGGTGGTGGTGGTGGTGGAAATAGGTGGCCCCGCCAAGAAACATTGGCTCTCTTGAAGATAAGATCAGATATGGATGGTGTGTTTCGTGATTCAAGTCTTAAGGGTCCACTTTGGGAAGAAGTTTCAAGGAAACTAGGTGAACTTGGTTATCATAGAAGTTCAAAAAAGTGCAAAGAGAAATTTGAGAATGTATACAAGTACCATAAAAGAACCAAAGAAGGTAGAAGTGGAAAATCAGAAGGAAAAACTTATAGATTTTTTGATGAGTTACAAGCACTTGAGAAAAAAATCACATTCTCTTCTACTTACCCTCCAAAACCACTTCCAAATAATAACACACTCTCATTACCACAAACAACAATGCCTAATGAAACCACAAAAACAATATCACATGTTACAAATATTACTATTCCATCCACAAACCCTACCACACTTATTTCTCCTTCACCACCGCCGCCAACGCCACCACCACAAACAACCAATGCAACAACCGCGACCAACCCTAGTAGCAACAACAATGTTTCATTTTCTTTACCGAACATGAACGTGAACCTATTCTCCACCACAACTACCTCAACTTCTTCTTCCACGGCTTCTGACGAAGACTTGGAAGAGAAGTACAGGAAGAAGAGAAAATGGAAAGACTATTTCAGAAGACTCACAAGAGAAGTGCTTGCGAAACAAGAGGACATGCAAAAGAGATTCTTGGAAGCCATAGACAAACGCGAAAAGGAACACATGGCTCAACAAGATGCTTTGAGAGTTCAAGAAATGGCGAGAATCAATAAAGAACATGAACTTCTTGTCCAAGAAAGATCATCAGCAGCTCAAAAAAACGCCGCGGTTATTACTTTTCTCCAGAAATTGTCCGGACAACCACCACCACAACCGCCGCCACCACCGCCACCACCACCAGCGTCAGAGCTGCCTCGAACTCCGGTATCACAAATACAAACACAGCAACTGGTGATACCAAACAACAACATTGTTGAGATTCTGAGTATGAATAATGGTCACAAAAGTTCTTGCGATGTTGCTTCTCCATCAAGGTGGCCAAAAAGTGAAGTTCATGCTCTAATAAGGATAAGAACAAGTCTAGAACCAAAGTATCAAGAAAATGGTCCAAAAGCACCATTATGGGAAGATATATCAGCTGGAATGAAGAGACTTGGCTACAATAGGAATGCGAAAAGGTGCAAAGAAAAGTGGGAGAATATCAACAAGTACTACAAGAAAATGAAAGAAAGTAACAAACAAAGGCGCGACGATAGTAAGACATGTCCTTATTTTCACGAACTCGAAGCTCTTTACAAAGAAAAGAACAAATTACAGAACCCTTTCGGTTCTAATTCGTTTCATAGTATAAAGTCAAGTGAAATGATGGAGCCATTGATGGTGCAACCAGAACAACAATGGAGACCTCCAACTCAATACGAAGAACCTCACCTGGTGAAGAAGAATAACAACGAAGAGAAAGAAGATGATAACGGTGATGTTGACGATGAAGAAGATGAAAACATGGATGACTACGAAGACGGAGATAGCATGGAAGAAGATGAAGGCGGTGTTAGCAGCCGTTATGAAGTTGTAACAAATAAACTTCCATCAATGAACACCGTTGGATGAGTTGGATGAGTCCATCATCCATCAGTACTGCAGTCTTTTAAGACAGATAGATTCTGAAAGAGAAATTTCTCTATCAGAATCTATCTGTTTCTACGACTGTAGTACACAAGAGAAAAGTTCAATTTGGTAATTTTAAAATTAAAAATTAAAATTTAAGTAAA
TARGET=204,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6531
PRIMER_LEFT_PENALTY=0.550932
PRIMER_RIGHT_PENALTY=0.102129
PRIMER_LEFT_SEQUENCE=GGCGAGGAAGATGATAAGATGA
PRIMER_RIGHT_SEQUENCE=CTTGAATCACGAAACACACCAT
PRIMER_LEFT=167,22
PRIMER_RIGHT=297,22
PRIMER_LEFT_TM=60.551
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=131
PRIMER_PAIR_PENALTY_1=0.6687
PRIMER_LEFT_1_PENALTY=0.550932
PRIMER_RIGHT_1_PENALTY=0.117817
PRIMER_LEFT_1_SEQUENCE=GGCGAGGAAGATGATAAGATGA
PRIMER_RIGHT_1_SEQUENCE=ATCACGAAACACACCATCCATA
PRIMER_LEFT_1=167,22
PRIMER_RIGHT_1=292,22
PRIMER_LEFT_1_TM=60.551
PRIMER_RIGHT_1_TM=60.118
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=0.8154
PRIMER_LEFT_2_PENALTY=0.550932
PRIMER_RIGHT_2_PENALTY=0.264444
PRIMER_LEFT_2_SEQUENCE=GGCGAGGAAGATGATAAGATGA
PRIMER_RIGHT_2_SEQUENCE=TCCTTGAAACTTCTTCCCAAAG
PRIMER_LEFT_2=167,22
PRIMER_RIGHT_2=332,22
PRIMER_LEFT_2_TM=60.551
PRIMER_RIGHT_2_TM=59.736
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=0.9412
PRIMER_LEFT_3_PENALTY=0.550932
PRIMER_RIGHT_3_PENALTY=0.390220
PRIMER_LEFT_3_SEQUENCE=GGCGAGGAAGATGATAAGATGA
PRIMER_RIGHT_3_SEQUENCE=GTTTCCTTGAAACTTCTTCCCA
PRIMER_LEFT_3=167,22
PRIMER_RIGHT_3=335,22
PRIMER_LEFT_3_TM=60.551
PRIMER_RIGHT_3_TM=59.610
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=1.0039
PRIMER_LEFT_4_PENALTY=0.550932
PRIMER_RIGHT_4_PENALTY=0.452945
PRIMER_LEFT_4_SEQUENCE=GGCGAGGAAGATGATAAGATGA
PRIMER_RIGHT_4_SEQUENCE=ACTTGAATCACGAAACACACCA
PRIMER_LEFT_4=167,22
PRIMER_RIGHT_4=298,22
PRIMER_LEFT_4_TM=60.551
PRIMER_RIGHT_4_TM=60.453
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_05364 Ps_contig_mira-and-tgicl-ass_franssen_37321
SEQUENCE=CGNATAATAAAAACCCTTTCATAGACTATACAAGTATACTATACTATTCCTCTTGTTTATTTTTTTTATTCTTTTTCTTCTTCTTCTCTCAAACTATAGGTGATGCAACAGTAGTTATTATGGATATTAATTCCGGTGAAAATATCATGCCGCCGGCGGTGAACGGCGGCGAGGAAGATGATAAGATGAATATGAATATCAACGGTGGTGGTGGTGGTGGAAATAGGTGGCCCCGCCAAGAAACATTGGCTCTCTTGAAGATAAGATCAGATATGGATGGTGTGTTTCGTGATTCAAGTCTTAAGGGTCCACTTTGGGAAGAAGTTTCAAGGAAACTAGGTGAACTTGGTTATCATAGAAGTTCAAAAAAGTGCAAAGAGAAATTTGAGAATGTATACAAGTACCATAAAAGAACCAAAGAAGGTAGAAGTGGAAAATCAGAAGGAAAAACTTATAGATTTTTTGATGAGTTACAAGCACTTGAGAAAAAAATCACATTCTCTTCTACTTACCCTCCAAAACCACTTCCAAATAATAACACACTCTCATTACCACAAACAACAATGCCTAATGAAACCACAAAAACAATATCACATGTTACAAATATTACTATTCCATCCACAAACCCTACCACACTTATTTCTCCTTCACCACCGCCGCCAACGCCACCACCACAAACAACCAATGCAACAACCGCGACCAACCCTAGTAGCAACAACAATGTTTCATTTTCTTTACCGAACATGAACGTGAACCTATTCTCCACCACAACTACCTCAACTTCTTCTTCCACGGCTTCTGACGAAGACTTGGAAGAGAAGTACAGGAAGAAGAGAAAATGGAAAGACTATTTCAGAAGACTCACAAGAGAAGTGCTTGCGAAACAAGAGGACATGCAAAAGAGATTCTTGGAAGCCATAGACAAACGCGAAAAGGAACACATGGCTCAACAAGATGCTTTGAGAGTTCAAGAAATGGCGAGAATCAATAAAGAACATGAACTTCTTGTCCAAGAAAGATCATCAGCAGCTCAAAAAAACGCCGCGGTTATTACTTTTCTCCAGAAATTGTCCGGACAACCACCACCACAACCGCCGCCACCACCGCCACCACCACCAGCGTCAGAGCTGCCTCGAACTCCGGTATCACAAATACAAACACAGCAACTGGTGATACCAAACAACAACATTGTTGAGATTCTGAGTATGAATAATGGTCACAAAAGTTCTTGCGATGTTGCTTCTCCATCAAGGTGGCCAAAAAGTGAAGTTCATGCTCTAATAAGGATAAGAACAAGTCTAGAACCAAAGTATCAAGAAAATGGTCCAAAAGCACCATTATGGGAAGATATATCAGCTGGAATGAAGAGACTTGGCTACAATAGGAATGCGAAAAGGTGCAAAGAAAAGTGGGAGAATATCAACAAGTACTACAAGAAAATGAAAGAAAGTAACAAACAAAGGCGCGACGATAGTAAGACATGTCCTTATTTTCACGAACTCGAAGCTCTTTACAAAGAAAAGAACAAATTACAGAACCCTTTCGGTTCTAATTCGTTTCATAGTATAAAGTCAAGTGAAATGATGGAGCCATTGATGGTGCAACCAGAACAACAATGGAGACCTCCAACTCAATACGAAGAACCTCACCTGGTGAAGAAGAATAACAACGAAGAGAAAGAAGATGATAACGGTGATGTTGACGATGAAGAAGATGAAAACATGGATGACTACGAAGACGGAGATAGCATGGAAGAAGATGAAGGCGGTGTTAGCAGCCGTTATGAAGTTGTAACAAATAAACTTCCATCAATGAACACCGTTGGATGAGTTGGATGAGTCCATCATCCATCAGTACTGCAGTCTTTTAAGACAGATAGATTCTGAAAGAGAAATTTCTCTATCAGAATCTATCTGTTTCTACGACTGTAGTACACAAGAGAAAAGTTCAATTTGGTAATTTTAAAATTAAAAATTAAAATTTAAGTAAA
TARGET=1107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5133
PRIMER_LEFT_PENALTY=0.140702
PRIMER_RIGHT_PENALTY=0.372557
PRIMER_LEFT_SEQUENCE=CGCGGTTATTACTTTTCTCCAG
PRIMER_RIGHT_SEQUENCE=ATTTGTGATACCGGAGTTCGAG
PRIMER_LEFT=1042,22
PRIMER_RIGHT=1152,22
PRIMER_LEFT_TM=60.141
PRIMER_RIGHT_TM=60.373
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=111
PRIMER_PAIR_PENALTY_1=0.6012
PRIMER_LEFT_1_PENALTY=0.140702
PRIMER_RIGHT_1_PENALTY=0.460467
PRIMER_LEFT_1_SEQUENCE=CGCGGTTATTACTTTTCTCCAG
PRIMER_RIGHT_1_SEQUENCE=TTGTTTGGTATCACCAGTTGCT
PRIMER_LEFT_1=1042,22
PRIMER_RIGHT_1=1182,22
PRIMER_LEFT_1_TM=60.141
PRIMER_RIGHT_1_TM=59.540
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.6150
PRIMER_LEFT_2_PENALTY=0.140702
PRIMER_RIGHT_2_PENALTY=0.474295
PRIMER_LEFT_2_SEQUENCE=CGCGGTTATTACTTTTCTCCAG
PRIMER_RIGHT_2_SEQUENCE=TGGTATCACCAGTTGCTGTGTT
PRIMER_LEFT_2=1042,22
PRIMER_RIGHT_2=1177,22
PRIMER_LEFT_2_TM=60.141
PRIMER_RIGHT_2_TM=60.474
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=136
PRIMER_PAIR_PENALTY_3=0.6150
PRIMER_LEFT_3_PENALTY=0.140702
PRIMER_RIGHT_3_PENALTY=0.474295
PRIMER_LEFT_3_SEQUENCE=CGCGGTTATTACTTTTCTCCAG
PRIMER_RIGHT_3_SEQUENCE=TTGGTATCACCAGTTGCTGTGT
PRIMER_LEFT_3=1042,22
PRIMER_RIGHT_3=1178,22
PRIMER_LEFT_3_TM=60.141
PRIMER_RIGHT_3_TM=60.474
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=137
PRIMER_PAIR_PENALTY_4=0.6417
PRIMER_LEFT_4_PENALTY=0.269153
PRIMER_RIGHT_4_PENALTY=0.372557
PRIMER_LEFT_4_SEQUENCE=GAGTTCAAGAAATGGCGAGAAT
PRIMER_RIGHT_4_SEQUENCE=ATTTGTGATACCGGAGTTCGAG
PRIMER_LEFT_4=963,22
PRIMER_RIGHT_4=1152,22
PRIMER_LEFT_4_TM=59.731
PRIMER_RIGHT_4_TM=60.373
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_05365 Ps_contig_mira-and-tgicl-ass_franssen_37342
SEQUENCE=GTCGGGGATCCCAACAATTCATCATCTCCTCTCCTCCTCAAATCACAAACCATACATAATATGCAAGACCTACGCCGTTACTCAAACGACACCGTTCATCTCGACCTCGGCACCACCACAACCACCACCATGATCACCACCACAACCTCTTCCTCCTCCTCCTCTTCCTTATCCATGGACATCGACGAATCCGCCGAAACGAAAATCCAACGTCTCATATCAGAGCATCCCGTCATCATCTTCACTCGATCTTCTTGCTGCATGTGCCACGTCATGAAGAAGCTTCTCTCCACCATCGGTGTTAATCCAACCGTCATCGAATTAGACGACAACGAGATCGCTTCTCTCCCTCATGATGACGATCACCTTGCTTTTGCTTCCGCTCTTCGTAACCGCTCTCCTGCTGTCTTCATCGGTGGCGCCTGCGTCGGCGGACTTGAGTCGCTTGTTGCTCTTCACGTCAGCGGTCATCTCGTCCCCAAACTTGTTCAAATCGGTGCTCTCTATGTATAACAATATCACTTTCAATTTATGTTTATTATTATTACTATTATTATTATTATTATTGAATCTTAAAAAACTGTATAAGTAATTGTATAATTTCTGGTAGTGTTTATGTGAAATGAAATTGCAATCTCATTTTAAAATTT
TARGET=151,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2645
PRIMER_LEFT_PENALTY=0.182682
PRIMER_RIGHT_PENALTY=0.081801
PRIMER_LEFT_SEQUENCE=TCTCCTCTCCTCCTCAAATCAC
PRIMER_RIGHT_SEQUENCE=ATTCGATGACGGTTGGATTAAC
PRIMER_LEFT=24,22
PRIMER_RIGHT=321,22
PRIMER_LEFT_TM=59.817
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=0.2801
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.242742
PRIMER_LEFT_1_SEQUENCE=TACTCAAACGACACCGTTCATC
PRIMER_RIGHT_1_SEQUENCE=CAGCAAGAAGATCGAGTGAAGA
PRIMER_LEFT_1=78,22
PRIMER_RIGHT_1=259,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.757
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=0.3167
PRIMER_LEFT_2_PENALTY=0.073989
PRIMER_RIGHT_2_PENALTY=0.242742
PRIMER_LEFT_2_SEQUENCE=GTTACTCAAACGACACCGTTCA
PRIMER_RIGHT_2_SEQUENCE=CAGCAAGAAGATCGAGTGAAGA
PRIMER_LEFT_2=76,22
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=0.3860
PRIMER_LEFT_3_PENALTY=0.182061
PRIMER_RIGHT_3_PENALTY=0.203971
PRIMER_LEFT_3_SEQUENCE=AAGACCTACGCCGTTACTCAAA
PRIMER_RIGHT_3_SEQUENCE=CTAATTCGATGACGGTTGGATT
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=324,22
PRIMER_LEFT_3_TM=60.182
PRIMER_RIGHT_3_TM=60.204
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=261
PRIMER_PAIR_PENALTY_4=0.4248
PRIMER_LEFT_4_PENALTY=0.182061
PRIMER_RIGHT_4_PENALTY=0.242742
PRIMER_LEFT_4_SEQUENCE=AAGACCTACGCCGTTACTCAAA
PRIMER_RIGHT_4_SEQUENCE=CAGCAAGAAGATCGAGTGAAGA
PRIMER_LEFT_4=64,22
PRIMER_RIGHT_4=259,22
PRIMER_LEFT_4_TM=60.182
PRIMER_RIGHT_4_TM=59.757
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=196
=
PRIMER_SEQUENCE_ID=SSR_05366 Ps_contig_mira-and-tgicl-ass_franssen_37342
SEQUENCE=GTCGGGGATCCCAACAATTCATCATCTCCTCTCCTCCTCAAATCACAAACCATACATAATATGCAAGACCTACGCCGTTACTCAAACGACACCGTTCATCTCGACCTCGGCACCACCACAACCACCACCATGATCACCACCACAACCTCTTCCTCCTCCTCCTCTTCCTTATCCATGGACATCGACGAATCCGCCGAAACGAAAATCCAACGTCTCATATCAGAGCATCCCGTCATCATCTTCACTCGATCTTCTTGCTGCATGTGCCACGTCATGAAGAAGCTTCTCTCCACCATCGGTGTTAATCCAACCGTCATCGAATTAGACGACAACGAGATCGCTTCTCTCCCTCATGATGACGATCACCTTGCTTTTGCTTCCGCTCTTCGTAACCGCTCTCCTGCTGTCTTCATCGGTGGCGCCTGCGTCGGCGGACTTGAGTCGCTTGTTGCTCTTCACGTCAGCGGTCATCTCGTCCCCAAACTTGTTCAAATCGGTGCTCTCTATGTATAACAATATCACTTTCAATTTATGTTTATTATTATTACTATTATTATTATTATTATTGAATCTTAAAAAACTGTATAAGTAATTGTATAATTTCTGGTAGTGTTTATGTGAAATGAAATTGCAATCTCATTTTAAAATTT
TARGET=536,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05367 Ps_contig_mira-and-tgicl-ass_franssen_37342
SEQUENCE=GTCGGGGATCCCAACAATTCATCATCTCCTCTCCTCCTCAAATCACAAACCATACATAATATGCAAGACCTACGCCGTTACTCAAACGACACCGTTCATCTCGACCTCGGCACCACCACAACCACCACCATGATCACCACCACAACCTCTTCCTCCTCCTCCTCTTCCTTATCCATGGACATCGACGAATCCGCCGAAACGAAAATCCAACGTCTCATATCAGAGCATCCCGTCATCATCTTCACTCGATCTTCTTGCTGCATGTGCCACGTCATGAAGAAGCTTCTCTCCACCATCGGTGTTAATCCAACCGTCATCGAATTAGACGACAACGAGATCGCTTCTCTCCCTCATGATGACGATCACCTTGCTTTTGCTTCCGCTCTTCGTAACCGCTCTCCTGCTGTCTTCATCGGTGGCGCCTGCGTCGGCGGACTTGAGTCGCTTGTTGCTCTTCACGTCAGCGGTCATCTCGTCCCCAAACTTGTTCAAATCGGTGCTCTCTATGTATAACAATATCACTTTCAATTTATGTTTATTATTATTACTATTATTATTATTATTATTGAATCTTAAAAAACTGTATAAGTAATTGTATAATTTCTGGTAGTGTTTATGTGAAATGAAATTGCAATCTCATTTTAAAATTT
TARGET=549,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05368 Ps_contig_mira-and-tgicl-ass_franssen_37345
SEQUENCE=ATTGAGTAAAAAAGAGTATTAATATATGAAAGTTATCCAAAAGAAAACGGTGAAGCTGCCTTCATTTGAAATCCATAATAAGATAGAAATCAAACCTTATCCTAAAAAGTTGTCCTCTAGAATTTAATTGTAAATAAGTGAATAAGTGCCTCAAGGCTAGGTACAAGCAAAAAGGAACACTGAATCTCCTTTCTAAGATTTCCTCTAACCCCACACGTTTTCTTCACCCCTTTCTCTCTCTCCAATTTCATTTAGTACTCATTATATCTCTCTTCTCAAGCCACCTTCCATCAAAGTCTCTTCAAACCTGTGACCTGCAAAGAGAAAAAATAGCTTATTTTAAAAGTACTTTTCAAAGCACTTAGAGAACTGAAAAACAACTAAAAAATTACTCAGAATTAAAACTTTATCCATAAGATTAACCTAAACATCCAAAAAGCACTTTTGACATTAGCTTAAAACAGAACAAACCAAATCCCACATAATCTGGGATGAGAAATTCAAAAAGGGAAGGAGTTTCTTAAGACTTAATCTTGTCTTCATAAACGATTCAAGGAGAGGTGAACAGAGAACATACACAGGAAAAGTAATGCTTCTCAAAAACCTAAACCACAACAACACAACAAAGCAATTTTTCTAAAAAAAACCTAAACAAGAACAGCAACAACAGATCAAAAGATTCTTTCTCAAACCCTAAACAAGAACACGAAGAACACACAAAAGTATTTTTTTTCTTAAACCAGAACAACAAAGCAAGAACACATCAATATTCTACAAAAAAACCTAAAAACGAAAACGAACGAAGGATGGCTTATCTGGAGACGGGAGAGAGAAGGCGT
TARGET=233,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3386
PRIMER_LEFT_PENALTY=0.206859
PRIMER_RIGHT_PENALTY=0.131699
PRIMER_LEFT_SEQUENCE=GGCTAGGTACAAGCAAAAAGGA
PRIMER_RIGHT_SEQUENCE=GAAGGTGGCTTGAGAAGAGAGA
PRIMER_LEFT=154,22
PRIMER_RIGHT=287,22
PRIMER_LEFT_TM=59.793
PRIMER_RIGHT_TM=60.132
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.4395
PRIMER_LEFT_1_PENALTY=0.384647
PRIMER_RIGHT_1_PENALTY=0.054900
PRIMER_LEFT_1_SEQUENCE=TCTAACCCCACACGTTTTCTTC
PRIMER_RIGHT_1_SEQUENCE=CCAGATTATGTGGGATTTGGTT
PRIMER_LEFT_1=203,22
PRIMER_RIGHT_1=489,22
PRIMER_LEFT_1_TM=60.385
PRIMER_RIGHT_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.4468
PRIMER_LEFT_2_PENALTY=0.315086
PRIMER_RIGHT_2_PENALTY=0.131699
PRIMER_LEFT_2_SEQUENCE=AGTGCCTCAAGGCTAGGTACAA
PRIMER_RIGHT_2_SEQUENCE=GAAGGTGGCTTGAGAAGAGAGA
PRIMER_LEFT_2=144,22
PRIMER_RIGHT_2=287,22
PRIMER_LEFT_2_TM=60.315
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=0.4468
PRIMER_LEFT_3_PENALTY=0.315086
PRIMER_RIGHT_3_PENALTY=0.131699
PRIMER_LEFT_3_SEQUENCE=GTGCCTCAAGGCTAGGTACAAG
PRIMER_RIGHT_3_SEQUENCE=GAAGGTGGCTTGAGAAGAGAGA
PRIMER_LEFT_3=145,22
PRIMER_RIGHT_3=287,22
PRIMER_LEFT_3_TM=60.315
PRIMER_RIGHT_3_TM=60.132
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=0.4468
PRIMER_LEFT_4_PENALTY=0.315086
PRIMER_RIGHT_4_PENALTY=0.131699
PRIMER_LEFT_4_SEQUENCE=AAGTGCCTCAAGGCTAGGTACA
PRIMER_RIGHT_4_SEQUENCE=GAAGGTGGCTTGAGAAGAGAGA
PRIMER_LEFT_4=143,22
PRIMER_RIGHT_4=287,22
PRIMER_LEFT_4_TM=60.315
PRIMER_RIGHT_4_TM=60.132
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_05369 Ps_contig_mira-and-tgicl-ass_franssen_37349
SEQUENCE=CAAGAGTGTAGTGAGAGTGCTGTTGGGATACTTTCAATTGTTTGTAGTGATTTTGGGAGTGCTAGAGAGGTAGCTATTGGAGCTGGTGTTTTGAGTCAGTTGTTGTTTCTTCTGCAGAGTCAGTGTGGTACTAAAACTAAAACTAAGGCAAGAATGTTGCTCAAGTTGTTGAGATCAAAGTGGACTGAGGAATCAATGCATTAGTGTGTGTTTGGAATCATGGTTATTTTACAAAATCACAGTTTTCTTGAAGATCCATAGTGTAGTTTTGGTAAATGTACTCTTGTGAATAATAATAATAATACATCATTATTAATCAGTATTTGATTTGATGGGTATGTGAGTATTTGTTTTTTGCTTTTATTGAATGGTAATGAATTCTCATAATATAACATT
TARGET=289,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05370 Ps_contig_mira-and-tgicl-ass_franssen_37352
SEQUENCE=GGGATCATTTGGTAACAACCTTCTTTCTTATTCTTGAACAAAGAGGTGAATTGACTGAGAATCAGAATGAAAAATGGAGCTCGTATCAGTATGTGGGTCGAACAGGTTCTGCAATTCCCACTGCTTCTTTGGCTGGAACTGATGTAAGTGTTGATGAAATCCGATCTGCTGCTGCTGTTTCTTCTGCTGCAGGTTACTATCCTCCTTCTCTTCATGGGGCTTTGGTTGGATCACCTGAGCCTGATCCCGCAGTGCAAGCTCTTGTTTATCAAGGCGGGTATGGAGGGGATTATGGAGGAACGAGGCCTGAATCCCAGAGGGAAACCTTGGATGAAGTAGAGATACGAGAGTTGCTGATTGATCATGTTGGTCATCGTTGTTGTTGGGGTAGTCGTCCTGCTCGAACATGGAAGATTCATGCAGTTGAAGACTGTAACGTTTATGTTGGAACTCTCGATACTTTTATAGAAGAAAGAGAGATCGTAAAAGAGACAGAGCCATACCTTGGTGGTAGTATTGATGGAAAGGATAATGGACCAGCACTTAGTATTTGGGAATTGGATCTAAGATCTCAGTTCCCTGTTCTATTTGTACCACACAAAGAAGTCCGTGAAAAGATTCCCCATTCTGAAATTATTGAGAAATGCTCAGGTTGTGCCGGACGAGGCGGGATAGTCTGCAGTACATGCAATGCTGACCAAGAACCTGGACATTATAAAGAAAATCAGATGACTCAGTGTCCTAGTTGTTATGGAAGGGGTTTGATTGCTCATAAAGATGGTTCTGACACACTATGTGTGAAATGTAATGGTAAAGGGAAGCTTCCTTGTACAACTTGTGGATCTCGTGGATTACTTAAATGTGAAACGTGTAATGGTAGTGGCTCTCTTTTAGCACGCAGTGTAGCCATTATTAAGTGGAAGACACTTTCAACTCGGAAAGTAAATGCAACAAGAGGAGCAGCATCAGTACCGGATGAGGTTTTCCACAGATCCCAAGGGGTTCAATTATGCAACACACAGGCTTACCAATGCACTCCTGCCTTTTTTGCTGATTTCTTTTTTTCTCAACAAGTTCTCTTCGGAAGTCATTGCTGAGAGNGCTNAAGTGCCGACCACTGCGAAGGGTCATC
TARGET=166,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1541
PRIMER_LEFT_PENALTY=0.154023
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=TGGAGCTCGTATCAGTATGTGG
PRIMER_RIGHT_SEQUENCE=CCAACATGATCAATCAGCAACT
PRIMER_LEFT=74,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=60.154
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=0.1682
PRIMER_LEFT_1_PENALTY=0.168114
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=TTGGCTGGAACTGATGTAAGTG
PRIMER_RIGHT_1_SEQUENCE=CCAACATGATCAATCAGCAACT
PRIMER_LEFT_1=128,22
PRIMER_RIGHT_1=369,22
PRIMER_LEFT_1_TM=60.168
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=242
PRIMER_PAIR_PENALTY_2=0.2535
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.000037
PRIMER_LEFT_2_SEQUENCE=TCTTTGGCTGGAACTGATGTAA
PRIMER_RIGHT_2_SEQUENCE=CCAACATGATCAATCAGCAACT
PRIMER_LEFT_2=125,22
PRIMER_RIGHT_2=369,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.000
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.2535
PRIMER_LEFT_3_PENALTY=0.253482
PRIMER_RIGHT_3_PENALTY=0.000037
PRIMER_LEFT_3_SEQUENCE=TTCTTTGGCTGGAACTGATGTA
PRIMER_RIGHT_3_SEQUENCE=CCAACATGATCAATCAGCAACT
PRIMER_LEFT_3=124,22
PRIMER_RIGHT_3=369,22
PRIMER_LEFT_3_TM=59.747
PRIMER_RIGHT_3_TM=60.000
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=0.2883
PRIMER_LEFT_4_PENALTY=0.154023
PRIMER_RIGHT_4_PENALTY=0.134318
PRIMER_LEFT_4_SEQUENCE=TGGAGCTCGTATCAGTATGTGG
PRIMER_RIGHT_4_SEQUENCE=ACCAACATGATCAATCAGCAAC
PRIMER_LEFT_4=74,22
PRIMER_RIGHT_4=370,22
PRIMER_LEFT_4_TM=60.154
PRIMER_RIGHT_4_TM=59.866
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_05371 Ps_contig_mira-and-tgicl-ass_franssen_37354
SEQUENCE=CTTCTTGGCAAGACTTGGCTGGTGGTGAGTACCRTCTTTAATACTTATATTTTCCTCATCGTCGACCTCAGGAATGACTGATACGTTTTCAGTAGTAGACATCTTAATCTTCTTCTGACTAGGGCTCTTAAGCTTCTTCGACGCCTTTCTCTTTTTCGAGGTTGTATTCCCATCCGATTTAGATTCCACGTATACCACATCTTCAGATTGATCATTATCATCAACATCATCATCTAGGGAAACAGTTTCATATTCTCGCCTCTCCTCTTGAAACCCCTCGATTGCAAGAACATCCAGAGCCTCTTTAGACCGATTCTCACGAGCTTGTAATGGCAGCATTGCAGGTTTACTATCAGTTAACCTACTTCCCTTTCTCCTCACACACACAA
TARGET=379,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05372 Ps_contig_mira-and-tgicl-ass_franssen_37363
SEQUENCE=ACTTATAAAAAATTAGTATCATGCATTCTTTAATGTTCATGATTTGCAATGAAAATAAAAACAGTAACCAATAACCAAAAGAACTAAATGGGTCCTTAAGAGATGCCAACAAGCTGTCGTCTCTCTCATACACACGATCAACATTTTTTTGTCTTATTTTGATTAATAACTATAAAGATCTCTGTTGGGATTCTTCATCAATGAAAATCTCTGAATCCCATTGAGTTTGAGTAACCAAATGCACATAAAATACAATATTCACACAGTAGAAAACTACACATAGAGGTTTTTGTTCTGTGATACTTCAAAGTTCCAAGCTTATATCAGAAAATCACTATCTAACATGACTACCAGAATCTTCAGGCATAGGAAATAGAAAATGTGGCATTGAATTAATCCTTGGAAGATTAAGAAGTAACTCTCCAGCTGATTCCTTCCTGGATAAACCATTTTCTTGCTGACTACCAGAATCGTCAGCATCATCATCTTGTTGAATTTCAAAATCAACCATTTTACTTTTGCATTCAGCATGATTGCTTGCATCAACTTTTCTATCTTCAGCTAAATCAAAAATATTACTGTTTCGAATAGTTTTGTTTTCGGCTGTAGAAGGGTCTTTCTGAAGAAGGTTGTAGAAGGAGTTCACCCTTGACATGACATTCTGTTCATCGGAGGCTGGTTGGATTTGAGAGTCGTTGAATAAATATTGTGTGAATTCCTCCAACTTTGCTCTGTCACTAGCGAAGCTGGAGTTTTCAGGTCCCATCTGTTCTGTAATGCAGTGTCCAATGTGATTAACCAGATCTTCAATCGACATAGAAGGGTGAAGACCAGGCAACTTGATCTGATCCAAATTGCTGAGAAACTTCAATGATTCAGCTCCTCTGCTCTTGAAACCTCTGATTGCATGAGGGTTCATCACTGAGCTAGGTGAGGTGATTTCTTGGAAATTGTTTTCAACAGCTTTACCCATAAGATTATGTTCACTTCTGGAAGAAGATGATTGACCTGCAGATCCCGATTCCACATCCTGCAATCCAAAAATCCCAGCATGCCCTTCACTTTTTCTATCAAAACCATCACTGGTTTCAATGTGGTCGTGAATTGCAGTTCCAGTCTCAAAATAAGGAGACTCCAACACAAAATCCTGCTGTTGGCTCAGAAAATTGAGACGGGGATCACACTGTATGAGCTTCTCAAAATGCCTCCCTAACAAACCTTGAGGGCATTGCATAAAGTGTCGCCTATGAATGCTGGCTTGTCCACCGGTAAAGTCTGATGTTGCTTGCCACAAGGTATGCTTCCTTGGTTGTGGATTGATCTCCCTAAAAAACAAAGGCCTCCTTGCAAGAACAACTTCCAGTGTTCCTGGTGCCTCCTCAGGGTAGTTAGCCTTAAGAGCCATGATATCAGACCATGGAATCTCAATCTTATTCTTAAGACAACCATCAAGAACTTCCCAAACAAGCTTATGCTTTGCAAAGTAACACTTTGCCACCAAATCTCCTTCATATCTAGATTTATACTCCCAAGTTCCAATTTTCAAAACAGTAGCAGGAAAATTAGAAGCCTTGAGTTTGGAATCAGCAGCAGCAGCTGCAGCAGCAGACTTCTGATCTTTCTTCTTAGAATCATGTTGCTCTGAAAGCCTCATCTGAATCAAATCCAAAAGTGAAGGACTCTTCTTCAGACGCAAACCAAGAGGACTTGGTTCATTCAAAGGGTTGTATAAACCCGGTGAAATCTGAACCGAATCATCGCTTAAATCACACTTGGGAGAAGAATCCAAATTGAAATCAAATCTAGGTCGTTTATTCGGAATAGCTCGAACATCATCATCTTGTTGCTGAGACTCCTCCAGTTTAACTTTTTTGATGAAGTGATTATCAGAATCAGATCTCATAAGCTGAACCATTGTTGAAGCTGATTTGTAAATTACTCAGAAGCGATTTTTTAACAATCGATTTTCAGAGAGAGAAAGAGTTGATGAAAGTGAAAATTGTGAATTGCGGCNTAGAAGAAAGAAGAAACAACAATAGAAGTTGTTTTTCGCTTCTTTGGCCAGCTGTC
TARGET=1585,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1596
PRIMER_LEFT_PENALTY=0.136083
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=GCAGGAAAATTAGAAGCCTTGA
PRIMER_RIGHT_SEQUENCE=TTTGGATTCTTCTCCCAAGTGT
PRIMER_LEFT=1553,22
PRIMER_RIGHT=1789,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.1809
PRIMER_LEFT_1_PENALTY=0.136083
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_LEFT_1_SEQUENCE=GCAGGAAAATTAGAAGCCTTGA
PRIMER_RIGHT_1_SEQUENCE=TGATTCAGATGAGGCTTTCAGA
PRIMER_LEFT_1=1553,22
PRIMER_RIGHT_1=1661,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.1943
PRIMER_LEFT_2_PENALTY=0.136083
PRIMER_RIGHT_2_PENALTY=0.058242
PRIMER_LEFT_2_SEQUENCE=GCAGGAAAATTAGAAGCCTTGA
PRIMER_RIGHT_2_SEQUENCE=GATTTAAGCGATGATTCGGTTC
PRIMER_LEFT_2=1553,22
PRIMER_RIGHT_2=1767,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.942
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=0.1973
PRIMER_LEFT_3_PENALTY=0.136083
PRIMER_RIGHT_3_PENALTY=0.061184
PRIMER_LEFT_3_SEQUENCE=GCAGGAAAATTAGAAGCCTTGA
PRIMER_RIGHT_3_SEQUENCE=CAATTTGGATTCTTCTCCCAAG
PRIMER_LEFT_3=1553,22
PRIMER_RIGHT_3=1792,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.939
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=0.2190
PRIMER_LEFT_4_PENALTY=0.195430
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=GCCACCAAATCTCCTTCATATC
PRIMER_RIGHT_4_SEQUENCE=CCTTTGAATGAACCAAGTCCTC
PRIMER_LEFT_4=1493,22
PRIMER_RIGHT_4=1722,22
PRIMER_LEFT_4_TM=59.805
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_05373 Ps_contig_mira-and-tgicl-ass_franssen_37365
SEQUENCE=ACTTTNCGACCTTTCTCTCCTYCTTCACGTTTACCCAATGGGAAGGCTATCCTCCACCATCGAAATAGCCCATCACCGCCGCAGACACGAAGATCGCCGCACTCCTGACTCCGATGTCTCCGAAGATCGCCGTCGCTACCGCCGTAGCCCTAGCTACGATTCATACGATCGTCACAGAGACCGCCGCCGCCGTAGCATATCACCCGAAAACAGAAACCCTAGACACGATAACATCAATCCAAACGGCAACGCTCTTCCTAAGAAATTCGGCCACCGCAACGGTTCCTATCTGGACCGTGACCGCAGCGATTTCAAGCGCTCCGAGTCTGAATCTGACGAAGAATTGAAGGGATTGAGTTTCGAGGAATACAGAAGGTTGAAGAGGCAAAAGATGAGGAAGTCGATGAAGTACTGTATCTGGAACGTTACGCCGAGTCCTCCGAGACGGGAGAACGAAGATATTGAGGATGTTTACAAAGCTGAAGAGATTTCTGAGAAATATGATGGGAAGGACGATAAGGAGGTTAAACCGAAAGCAAAATCTGAATCCGTGTCCGATGAATCTGATGGTAGTGACTCGGATAATCCACGTTTGAGGAAGAAGAGAAGTAAAAGTTCTTCTAAGAAATCGCGAAGCCATAGTGAATCGGAATCAGAAGAAGATGAAGATCTGAAAAGGGGAAAGAATAGGAATTATAGTGACAGCGATAGCGATAGTGAATCAGAGGAGGAAGATCGGAAGAGAAGAAGGAGAAGCAAGAGGAAGATCAGTAGAAGGAAAAGGAAAAGTAATCGGAGAAAGAGAAGGCATAGTGATTCAGAAGAGAGTGAGAGTGATGACTCTGACTCTGATGGCAGTGAAAGAAGGGAAGCATTCTTCTAGGTCTCGATCAAAACGAAGCAGGAAAGGAACTAAGAAAAGTGCTGAACCGATAAGTGAGAATTCGGAAGATAATGAGATGGTTTCTGATTCAGCTGTTGTGAAGAATAAGGAAGCAATTGTTGTTGATGAGGTGGATGTGCCTGAGATTAATGCTGAGGCTCTACAATTAAAGGAATTGTTAGAGTTACAGAAGAAACCGGCTTTGGATAACGAGGCGACAGTTGGGCCAATGCCTCTGCCAAGAGCCGAGGGGCATATAAGCTATGGTGGTGCACTTAGGCCTGGTGAAGGAGATGCTATTGCACAATATGTTCAACAAGGGAAACGTATTCCTCGTAGAGGAGAAGTGGGTCTTTCTGCAGAAGAGATTTCAAAGTTTGAGGAGCTTGGTTATGTGATGAGTGGCAGTAGGCATCAGAGGATGAATGCAATTCGTATCAGGAAAGAAAATCAAGTTTATAGTGCTGAAGATAAGCGTGCTTTGGCTATGTTTAACTATGAAGAAAAGGCTAAAAGAGAACATAAGGTTATGGCTGATTTGCAGCGGCTAGTACAGCGTCACATTGGTCAGGATGTTGGTCCAACTCATGATCCTTTTGGCGCAAAAGACGGTGCTGATGCCTGATATTATTATTCATGTATGTCAGCTTGTTTTCTTTTGCTTTATTTGGTTGTTTTTGTTCRGCTGGCTATGTTATTTAGACAAACCTGTTTAGACCGTTTAAATAGAGACGAGAACTTATTGCCTTCGTCAACATAGCATATGGATATTCAGAAAGTGGTGTATGATTTTCAATTAGTGACTAACTAGTATATAAACAGTTTGGTTACNAGTTAATACTGTGTCCATCTGTTTATGTGAAATTGCTACAGAAGGGTAGATTTATCATTTATTGCAA
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05374 Ps_contig_mira-and-tgicl-ass_franssen_37369
SEQUENCE=TGGCGGTTACCGGTAACGGACTTTCCGCCTCTTGTTCACCGTTTCCGGCGAGATTGACGTTAATTTTGTTCTTCTTGGAATCACCAGCGGTGAATTCTACCGGAGACCAGAATTCAGTGAGAGGTGCGGGTAGGTTGAGGTCAAAGCCACGGTGGCTGATGGAAGAGGACGCGGCGGTGACGTCGGAGATTGTGATGTTACTGCTGTTATTAGCGTTGGCGTTGCTGTTACTGATATTGTGATTGTTGTTGTTGCTGTTGTTGTTACCTCCTTCGTAGTGGCAGCGTTTGTGTCCACCAAGAGCTTGACCAGTGGGGAAACTCTTGTGACAGATGGAACACTCGTGCATCTTTCCGGTGGTGGTGACAGTTGAGACGTTGACGCTGTCGTTGACGGCGGTGGATTGGTTTTCTAATGAGGATTTGCGATGGCTGGCTTTGTGGCCACCGAGTGCTTGATAAGAAGGGAAAGCTTTGTTGCAGACGGTGCACCTGTGATTCAGCTTCGCAGGCGGTGGTGGTGGTGGCGGAGAGTTTTGATGTTGTTGTTGTTGCTGTGATTGCGATTGTGAAGATGATGATTCTTGTTGTTGAGATCGACTCTTGTTGATGGTGTTGTTGGTGTTCCCTTGTGCGAGCATGATGAGACAAAGAGCGAGATATTCTTCTTCGGTGGGTGGATTCCCAATCCGCGGTCGTTTAGATCGTTTCTTCTTAACCAAAGAGTCGTGTTCGTGCTGGTCAAGATTCTTTTGATTCTCTGATTGGTTTTTGAAGATTGGGAATGGAATAGGAGCTTGTGTTGTTGGAGAAGAATTCATAGCTTGTAACTCTAAAGCCATTGAAATAGGTGAAAGGAAAGAAGAAGAAAAAAGTGTTGAGTGAGTAATGAGATTGAGATGAGTCGNYGGACCCCG
TARGET=243,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05375 Ps_contig_mira-and-tgicl-ass_franssen_37369
SEQUENCE=TGGCGGTTACCGGTAACGGACTTTCCGCCTCTTGTTCACCGTTTCCGGCGAGATTGACGTTAATTTTGTTCTTCTTGGAATCACCAGCGGTGAATTCTACCGGAGACCAGAATTCAGTGAGAGGTGCGGGTAGGTTGAGGTCAAAGCCACGGTGGCTGATGGAAGAGGACGCGGCGGTGACGTCGGAGATTGTGATGTTACTGCTGTTATTAGCGTTGGCGTTGCTGTTACTGATATTGTGATTGTTGTTGTTGCTGTTGTTGTTACCTCCTTCGTAGTGGCAGCGTTTGTGTCCACCAAGAGCTTGACCAGTGGGGAAACTCTTGTGACAGATGGAACACTCGTGCATCTTTCCGGTGGTGGTGACAGTTGAGACGTTGACGCTGTCGTTGACGGCGGTGGATTGGTTTTCTAATGAGGATTTGCGATGGCTGGCTTTGTGGCCACCGAGTGCTTGATAAGAAGGGAAAGCTTTGTTGCAGACGGTGCACCTGTGATTCAGCTTCGCAGGCGGTGGTGGTGGTGGCGGAGAGTTTTGATGTTGTTGTTGTTGCTGTGATTGCGATTGTGAAGATGATGATTCTTGTTGTTGAGATCGACTCTTGTTGATGGTGTTGTTGGTGTTCCCTTGTGCGAGCATGATGAGACAAAGAGCGAGATATTCTTCTTCGGTGGGTGGATTCCCAATCCGCGGTCGTTTAGATCGTTTCTTCTTAACCAAAGAGTCGTGTTCGTGCTGGTCAAGATTCTTTTGATTCTCTGATTGGTTTTTGAAGATTGGGAATGGAATAGGAGCTTGTGTTGTTGGAGAAGAATTCATAGCTTGTAACTCTAAAGCCATTGAAATAGGTGAAAGGAAAGAAGAAGAAAAAAGTGTTGAGTGAGTAATGAGATTGAGATGAGTCGNYGGACCCCG
TARGET=513,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05376 Ps_contig_mira-and-tgicl-ass_franssen_37369
SEQUENCE=TGGCGGTTACCGGTAACGGACTTTCCGCCTCTTGTTCACCGTTTCCGGCGAGATTGACGTTAATTTTGTTCTTCTTGGAATCACCAGCGGTGAATTCTACCGGAGACCAGAATTCAGTGAGAGGTGCGGGTAGGTTGAGGTCAAAGCCACGGTGGCTGATGGAAGAGGACGCGGCGGTGACGTCGGAGATTGTGATGTTACTGCTGTTATTAGCGTTGGCGTTGCTGTTACTGATATTGTGATTGTTGTTGTTGCTGTTGTTGTTACCTCCTTCGTAGTGGCAGCGTTTGTGTCCACCAAGAGCTTGACCAGTGGGGAAACTCTTGTGACAGATGGAACACTCGTGCATCTTTCCGGTGGTGGTGACAGTTGAGACGTTGACGCTGTCGTTGACGGCGGTGGATTGGTTTTCTAATGAGGATTTGCGATGGCTGGCTTTGTGGCCACCGAGTGCTTGATAAGAAGGGAAAGCTTTGTTGCAGACGGTGCACCTGTGATTCAGCTTCGCAGGCGGTGGTGGTGGTGGCGGAGAGTTTTGATGTTGTTGTTGTTGCTGTGATTGCGATTGTGAAGATGATGATTCTTGTTGTTGAGATCGACTCTTGTTGATGGTGTTGTTGGTGTTCCCTTGTGCGAGCATGATGAGACAAAGAGCGAGATATTCTTCTTCGGTGGGTGGATTCCCAATCCGCGGTCGTTTAGATCGTTTCTTCTTAACCAAAGAGTCGTGTTCGTGCTGGTCAAGATTCTTTTGATTCTCTGATTGGTTTTTGAAGATTGGGAATGGAATAGGAGCTTGTGTTGTTGGAGAAGAATTCATAGCTTGTAACTCTAAAGCCATTGAAATAGGTGAAAGGAAAGAAGAAGAAAAAAGTGTTGAGTGAGTAATGAGATTGAGATGAGTCGNYGGACCCCG
TARGET=540,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05377 Ps_contig_mira-and-tgicl-ass_franssen_37371
SEQUENCE=AAGTTGCCGGGGAAGTGATTTTATTGATAACATATGTTCAGATGTTAGGGAGAATTGTTCCTACGTGATACATGTACCATTTGCAATTATGACACTGCGTCTGTGTTTCTCCATTCCGCACAAAAAAACTAGTTGTATATATGAATATTTACAAACCTTATGTTAGAGATGATGACATATGAAACACTACTATACAAATCTGAGTGACATTATTCTCGTTTATCCTCCTCAATATCTTCTGCAATTGTAAGATTTGTGGCACTAGAACCTTTTCTTTCTTTCCGCTCAAGATTGGCTGTCACATCCCCACTCATCTTTGCAATGCTGCTTGTCAGAGAGATACCTTCCTCATCAATGTCATCTGTTCCCTCTAAGTATGCATATCTAGTAGGGTTACGAGAAGGAGCCCAAAGTACACATATCACCAGCAAGAGAGTATATGAAAGCAGGGACCAAAAAGCGGGAATAATCCAAGCAATTTGCCACAGCTCACTTAGAGGATCAGTTGCATTGAAGTACAGCTCAAAGCCAATCCATGCAATGGACAGTAGCACAGACACTGCCAGGGTATTTGTAAATTTTCGGTAGATCTCCAGTTTAGCTAAGTTTCTCCTTGTCTGCAGTTTCTCCAAGGTTTTAGACAAGGAAGAGAAGATCCATAGAATGAAGCATGAGTCAAGGCAAACAACAGGCAGTACCAACAGCAACTTAGATTTCCCAGAGAAGTCATTGATGTTCCCCAAATGCTCAACAAGCTCAAACGCTTCACATGCAACAAAGTACATCACCCCAAGAAGCACAACTCTATGGGCAACCCCTTGCGCGCCAAGTGTAGGACGAACAACACCATATCCCATAGATACAACAAGCAAAAGCAGCCTCGAAAGTGTCTTCTTAACAGAGGTGAAAGTAACCGCCCATATGGTAATTCCCATAGGCCTACTACCAGTAACATTGAAATTGGCATACTCAAAATACCATAAAGCCATCTCACACATTCCAAGAGCAATAACAGCAGTAATGTGGTAGTGAAGGTGAACATGAATAATATCCTTCTCTTTCCAAAACTGAAGAAACCTCAAAAACCATAGAAGACCAAGTAACAGATAAGCCAAAGACATGAAACCGTAAAGGGTCATAAGAGGTGCCATTTTACCAGGAAGATAACCATTAGGATTTCTCCATACAGTTTTTCCTGTAACAGTAGTCCCTTTCAGGTTAGGATCACAAAACATGAAGTAAAGATAATACATCCCAGTAGCATTGATTTCAACAATTTGAGGATCCATATGAGCAACTTCACCGATTCCTCGGAAGAAGGTTTTGATCCGTTTGGGAAAGTCAGGATTATCAGGATTATTATGAATGATAACTTCACCAACATTACAGTTATATTCTTTAGCAAGATCAGGGGTACAGCAAATTAAATCAGACTTGGGATTAAAATAGAACCCACCGATTCTGTTTCTGTCTTTAACTTCAAGAATAATTGCTTCAACAAGACCAGTATGGGTTTGCATTTGGCCATAGGAGATAGCAGATTCTCTGGGTCTGTTGAACGACACCGTTTCGAACCTGATGAAAGATGAAGAAGAGGAGAAGATAGCTTCTGTTCCGGCGTGGAAGAAGAAAGCGTTGGAACGGTGAGTGAAGGGTTCGTTGTTGTAGTGATGAATTGAAGCATTGACGTTTACAAGAATGGTAAGAAAGAGTGCATAATAGATCAAGTTGACACTCTTGAACCCCATCATCATCAGAATGAAGAAAGAGAAAAATGAGAGAGCATCGATAGTAGTAACAAGTTAGGTTGAGTAAACGTTAAGTTGACTGTGATTCTGGATTCATCTCTCACTCACCTCGTCTCAACCTTTTCAACTTTAACACCATGGCCACTCTATCTCTATCAAT
TARGET=271,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3579
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.355573
PRIMER_LEFT_SEQUENCE=AGGGAGAATTGTTCCTACGTGA
PRIMER_RIGHT_SEQUENCE=GGTATCTCTCTGACAAGCAGCA
PRIMER_LEFT=46,22
PRIMER_RIGHT=343,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.644
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=0.4961
PRIMER_LEFT_1_PENALTY=0.347645
PRIMER_RIGHT_1_PENALTY=0.148450
PRIMER_LEFT_1_SEQUENCE=CTGCAATTGTAAGATTTGTGGC
PRIMER_RIGHT_1_SEQUENCE=GGCTCCTTCTCGTAACCCTACT
PRIMER_LEFT_1=238,22
PRIMER_RIGHT_1=407,22
PRIMER_LEFT_1_TM=59.652
PRIMER_RIGHT_1_TM=60.148
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=5.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.5164
PRIMER_LEFT_2_PENALTY=0.160828
PRIMER_RIGHT_2_PENALTY=0.355573
PRIMER_LEFT_2_SEQUENCE=ATGACACTGCGTCTGTGTTTCT
PRIMER_RIGHT_2_SEQUENCE=GGTATCTCTCTGACAAGCAGCA
PRIMER_LEFT_2=88,22
PRIMER_RIGHT_2=343,22
PRIMER_LEFT_2_TM=59.839
PRIMER_RIGHT_2_TM=59.644
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.5585
PRIMER_LEFT_3_PENALTY=0.347645
PRIMER_RIGHT_3_PENALTY=0.210886
PRIMER_LEFT_3_SEQUENCE=CTGCAATTGTAAGATTTGTGGC
PRIMER_RIGHT_3_SEQUENCE=GGATTGGCTTTGAGCTGTACTT
PRIMER_LEFT_3=238,22
PRIMER_RIGHT_3=534,22
PRIMER_LEFT_3_TM=59.652
PRIMER_RIGHT_3_TM=59.789
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.5605
PRIMER_LEFT_4_PENALTY=0.204906
PRIMER_RIGHT_4_PENALTY=0.355573
PRIMER_LEFT_4_SEQUENCE=GCAATTATGACACTGCGTCTGT
PRIMER_RIGHT_4_SEQUENCE=GGTATCTCTCTGACAAGCAGCA
PRIMER_LEFT_4=82,22
PRIMER_RIGHT_4=343,22
PRIMER_LEFT_4_TM=60.205
PRIMER_RIGHT_4_TM=59.644
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_05378 Ps_contig_mira-and-tgicl-ass_franssen_37375
SEQUENCE=CCNAACAAAAATACAGTATTTTTTCCTTTTTTTTTTAATTTCACTATTGATTGTATGAATAGCATTACTCAAGTTCTAAATCATAGAACCACTTTTGTTGTGATACAGGAACATGATACATAGTTTTGTAAGAAATGGTATCAGTTTCAAATTGAAACTTCTATATTCACATAGGTTACTTAGAAGGAATAAGTGTTTGACATTACACCTCAAAAACCAAGGAAAAATAATTCACTTTCCATACAGAATTCCTAGCTTTCATACCCTGGATTTGAATCTCCCAGAGGCGATGGTGTTGCTTCAATTTTTGTTAAAACTTCTGGAATCCCAGATGTGTTCCTTATTATAAGTATCTCCTCATCGAGATAAGAAATCATGAAGAGTCTCTGAAAGCTTCCACTTAGAGGAACTTTTAGTCCATTGGAAAGAGCATCGCGTAAAGGTACCGGTAGCTGTTGTAAAACATTAACTGCCTGTCCAAGTGCACCTTTGAGCTGTTCTGGTACTCTGTTTTCCAAAACTGTTGGTGACACAAGAATCCCTTCTACATACTCCTCTTTCATTCTAAGTGGCCCCTCGACAGTTAACTTGGTTGAGAGAATAACACTGCTTTCTATCGAGTTTAAAAATGTTGTGTTTGCCGTAATCTTTGCATTTCCGTCCTTGATGAAGACATCCATTTTCGACAAATTAGCTAAACTTGAAGGAAAATTCTCAAATATAACCCTAGCCGCAAACAACCCGAGAGATCCAGGACCAAACCACTCAAATTTCCATCGACCTTCTAGAAAAGGTGACGTCGTGGGTCGAGGTGTTGGATTGAGCCGCTCAAGACCAGTTATCCTCTCATTCACATCAATTCTTTGATATTCGGTTGTCTTCCCAGAGACTAAGCCCTCAAACTCACCTGCATCTTTAAACGCAAGGAGAAGCGATTCTTTGGCGGATAGGCGTTCCATTTCCTTAGCATACGCGGCGGGTGCGCCAGTTTGAATTTGAACCATAGCTCTACAATAGGAACGTCTCTGCCACTTGACGCGTGAGGATGAGACTTTCCATGAAGAAGAAGAAGATGAAGATGATGATGAAGGGATTGAACAATTGGTGATAGATGAAATACAGGGATGAGAAGCAGAAACAGAAGTAGCCATTGAAAGAGAATTGAAGTTGTTTGGGTTCAATTTTTCATGAGTTAGTTCTTAACATGAAATGAAAGTGTTGCTCATCTCGGGTAGGCGTCTGTGTGTGGTGTTTTCCTTTCCCCCT
TARGET=1060,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2962
PRIMER_LEFT_PENALTY=0.003820
PRIMER_RIGHT_PENALTY=0.292414
PRIMER_LEFT_SEQUENCE=GCGTTCCATTTCCTTAGCATAC
PRIMER_RIGHT_SEQUENCE=TGCTTCTCATCCCTGTATTTCA
PRIMER_LEFT=950,22
PRIMER_RIGHT=1133,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.708
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.3860
PRIMER_LEFT_1_PENALTY=0.003820
PRIMER_RIGHT_1_PENALTY=0.382228
PRIMER_LEFT_1_SEQUENCE=GCGTTCCATTTCCTTAGCATAC
PRIMER_RIGHT_1_SEQUENCE=TCTGTTTCTGCTTCTCATCCCT
PRIMER_LEFT_1=950,22
PRIMER_RIGHT_1=1141,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=60.382
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=192
PRIMER_PAIR_PENALTY_2=0.4069
PRIMER_LEFT_2_PENALTY=0.114470
PRIMER_RIGHT_2_PENALTY=0.292414
PRIMER_LEFT_2_SEQUENCE=TTGTCTTCCCAGAGACTAAGCC
PRIMER_RIGHT_2_SEQUENCE=TGCTTCTCATCCCTGTATTTCA
PRIMER_LEFT_2=874,22
PRIMER_RIGHT_2=1133,22
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=59.708
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.4762
PRIMER_LEFT_3_PENALTY=0.183834
PRIMER_RIGHT_3_PENALTY=0.292414
PRIMER_LEFT_3_SEQUENCE=TTTGATATTCGGTTGTCTTCCC
PRIMER_RIGHT_3_SEQUENCE=TGCTTCTCATCCCTGTATTTCA
PRIMER_LEFT_3=862,22
PRIMER_RIGHT_3=1133,22
PRIMER_LEFT_3_TM=60.184
PRIMER_RIGHT_3_TM=59.708
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=272
PRIMER_PAIR_PENALTY_4=0.4819
PRIMER_LEFT_4_PENALTY=0.189529
PRIMER_RIGHT_4_PENALTY=0.292414
PRIMER_LEFT_4_SEQUENCE=ACTAAGCCCTCAAACTCACCTG
PRIMER_RIGHT_4_SEQUENCE=TGCTTCTCATCCCTGTATTTCA
PRIMER_LEFT_4=888,22
PRIMER_RIGHT_4=1133,22
PRIMER_LEFT_4_TM=59.810
PRIMER_RIGHT_4_TM=59.708
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_05379 Ps_contig_mira-and-tgicl-ass_franssen_37385
SEQUENCE=TACTGTTTAATATCAATTCATTGATATCAATTTACAAGATGAAACAAGCANGGGTTGAAGCCGGTGCAACACTTGGTAAGATATATTATAACATTGCAAAGAAAAGCAATTCTCTTGCTTTTCCAGCTGGGGTTTGTTTTTCTTTAGGTGCTGGTGGTCATTTTTCAGGTGGTGGCTATGGAAATTTGTTGAGAAAATTTGGTCTTTCTGTTGATAATATAATTGATGCGAATATCGTTGATGTCAATGGTAACATCCTTGACAGAAAATCAATGGGAGAAGATCTCTTTTGGGCTATAAGAGGTGGTGGTGGTGCTAGTTTTGGTGTTATTCTTTCATGGAAACTCAAATTGGTTCAAGTTCCTCCACAAGTTACTGTTTTCAATGTGAAAAGGAATGTGGATGAAGGTGCAACTGATGTTGTTTACAGATGGCAAATAGTTGCACCAAAGTTGCATAAAGATCTTTTCATTAGATTGGAACCTAATATTGCTCAAATTGGTAAAGAAGGTAAAACAGACGTGCAAGTTAGTTTTATCGGTCAATTTTTAGGGACAATTGAAAGACTATTACCTTTGATAAGTGACAGTTTCCCTGAATTAGGATTGAAAAAAAGTGATTGTCTTTCAATGCCTTGGGTTAATACCACTCTTTTTTGGTATAGTAACCCAATTGGTACTCCCCTTGAAGCATTGTTAGATGAACCAAAAGAACCTGAAACAACTTATTCCAAAAGTCAATCAGATTATGTGAAGAGGCCTATTTCAAGGGAAGGTTTAGAATCTATATGGAAAAAAATGATTGAAGGTGGAAGTTTGTTTATGCAATGGAATCCTTATGGCGGAAGAATGGAAGAGATATTGCCATCAGAAACACCGTTTCCTCATAGGGCTGGAAATTTGTTCAAGATTCAATATCTTAATTATTGGACTGATGAATCTTCCGAGCAACGTCATATGAATATTTCAAGGTCTTTTCATGAATTTATGACGCCCTATGTTTCAAATTCTCCGAGGGAGGCATTCCTCAACTATAGAGATGCTGATATTGGTGCCAATCATCCAAGTAATACAACCAACATTGACATTGCTAGAACTTATGGAAGCAAGTATTTTAAAGAAAATTTTAAAAGACTAGTTACCGTGAAAAACAAAGTTGATCCCGAGAATTTTTTCAGATATGAACAAAGTATACCTACTAGGACATATTAAAGTTATAAAGTGTTGTATTTTACTAAATAAAAGTGATAAATTATAGTTTGAATTTTTAAGATAAAATTATGTAAAATATATCCTGAGAAATAAATAGAAAAATAAAATTCCTAANAACTACAAA
TARGET=303,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2560
PRIMER_LEFT_PENALTY=0.035021
PRIMER_RIGHT_PENALTY=0.220992
PRIMER_LEFT_SEQUENCE=TTTTTCTTTAGGTGCTGGTGGT
PRIMER_RIGHT_SEQUENCE=CACCTTCATCCACATTCCTTTT
PRIMER_LEFT=136,22
PRIMER_RIGHT=410,22
PRIMER_LEFT_TM=60.035
PRIMER_RIGHT_TM=60.221
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.2560
PRIMER_LEFT_1_PENALTY=0.035021
PRIMER_RIGHT_1_PENALTY=0.220992
PRIMER_LEFT_1_SEQUENCE=GTTTTTCTTTAGGTGCTGGTGG
PRIMER_RIGHT_1_SEQUENCE=CACCTTCATCCACATTCCTTTT
PRIMER_LEFT_1=135,22
PRIMER_RIGHT_1=410,22
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=60.221
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.3368
PRIMER_LEFT_2_PENALTY=0.115769
PRIMER_RIGHT_2_PENALTY=0.220992
PRIMER_LEFT_2_SEQUENCE=GTGGTGGCTATGGAAATTTGTT
PRIMER_RIGHT_2_SEQUENCE=CACCTTCATCCACATTCCTTTT
PRIMER_LEFT_2=168,22
PRIMER_RIGHT_2=410,22
PRIMER_LEFT_2_TM=60.116
PRIMER_RIGHT_2_TM=60.221
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=243
PRIMER_PAIR_PENALTY_3=0.3533
PRIMER_LEFT_3_PENALTY=0.021195
PRIMER_RIGHT_3_PENALTY=0.332137
PRIMER_LEFT_3_SEQUENCE=AGCAATTCTCTTGCTTTTCCAG
PRIMER_RIGHT_3_SEQUENCE=GGAGGAACTTGAACCAATTTGA
PRIMER_LEFT_3=104,22
PRIMER_RIGHT_3=366,22
PRIMER_LEFT_3_TM=60.021
PRIMER_RIGHT_3_TM=60.332
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=0.3672
PRIMER_LEFT_4_PENALTY=0.035021
PRIMER_RIGHT_4_PENALTY=0.332137
PRIMER_LEFT_4_SEQUENCE=GTTTTTCTTTAGGTGCTGGTGG
PRIMER_RIGHT_4_SEQUENCE=GGAGGAACTTGAACCAATTTGA
PRIMER_LEFT_4=135,22
PRIMER_RIGHT_4=366,22
PRIMER_LEFT_4_TM=60.035
PRIMER_RIGHT_4_TM=60.332
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_05380 Ps_contig_mira-and-tgicl-ass_franssen_37386
SEQUENCE=GACTTTCACTCTTTCCGTCGAGAAAGAACAAACGCACCATAATGTATCGCATTCTCCACCGCAGCTTCTGCACCATTGCCGAAGAATCAACCACCAGCATCAAGTCCATTTCACAAGACCTCTACAAAGAACAAAACCTCAAGACACTCGTTGACAAGTTCAAGAAAGCTTCTGACATCGACCGCTTCCGTACAAAAGCCGGCATCTACGAGGACACCGTCCGCCGCCTCGCCAGAGCTAAACGCTTCCGATGGGTCCGTGACATCATCGAACACCAGAAGACTTACACCGACATAGGCAATGAAGGTTTCTCAGCGCGTCTCATTACGTTGTATGGGAAATCCAAAATGGATAGACACGCACAGAAGCTGTTTGATGAAATGCCTCAGAGAAACTGTACGCGTTCTGTGTTATCTCTTAATGCTTTGTTAGCTGCTTATCTTCATTCCAAGAAATATGATGTTGTTGAGAAGCTTTTTAGGGATCTTCCTGTTCAGCTCTCTGTGAAACCTGATTTGGTTTCTTATAATACTTTAATAAAAGCGTTGTTGGAAATGGGTTCTTTTGATTCTGCTGTTGCTGTGGTTGAGGAGATGGAGAAGGAAGGATTGAAAACTGATTTGATCACGTTTAATACTCTGCTTGATGGGTTGTACTCAAAGGGTCGTTTCGAGGATGGTGAGAAACTGTGGGGGAAATTGGGTGAGAAGAATGTTGTTCCTAATATCAGGACTTACAATGCAAGGTTGTTGGGGTTGGCTATGGTGAAGAAAACTGGTGAAGCTGTTGAGTTTTATGAGGAAATGGAGAAAAAGGGTGTCAAGCCTGATATTTTTAGCTTGAATGCTTTGATCAAAGGTTTTGCGAATGAAGGGAACTTGGATGAAGCTAAGAAGTGGTTTGGTGAAATTGGGAAATCTGAGTATGACCCTGATAAGGCCACTTATTCAATAATTGTTCCTTTTCTGTGTGAGAAGGGTGATTTGAAAACCGTCCTTGAGATGGTTAAGGAGATATTCTACACTCACTGCCGTGTTGATGCATCGTTGCTGCAAGTTGTGGTGGACAAACTGCTGAGTGAGTCAATGGTTTCAGAGGCCAAGGAGATTGTGGAACAAGGGAAAACAAATAAATACTGTCGCTATAAGCTAAATTTGCCTGCAGATGAGTGAGTGAGTAGTAGCTTTTGTTTGTTGTGTCCATTATTATTAATTTTGAACACTATATGTTTTACTAGTCATAATGTTGAATTGGCGATGCCTCCATGAGTGGATATTGTTTGGCAATCTGGGCTTTGTAAACTGATTGCTTGAAAATGCCATTTTCTGTTGGATGATTATTTGTTCTTTTTCCTTGTTGAATATCAACCCTTAAACTTTAACCCT
TARGET=1166,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4525
PRIMER_LEFT_PENALTY=0.237277
PRIMER_RIGHT_PENALTY=0.215225
PRIMER_LEFT_SEQUENCE=CTACACTCACTGCCGTGTTGAT
PRIMER_RIGHT_SEQUENCE=AACAATATCCACTCATGGAGGC
PRIMER_LEFT=1018,22
PRIMER_RIGHT=1279,22
PRIMER_LEFT_TM=60.237
PRIMER_RIGHT_TM=60.215
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=0.5141
PRIMER_LEFT_1_PENALTY=0.298898
PRIMER_RIGHT_1_PENALTY=0.215225
PRIMER_LEFT_1_SEQUENCE=TGCTGAGTGAGTCAATGGTTTC
PRIMER_RIGHT_1_SEQUENCE=AACAATATCCACTCATGGAGGC
PRIMER_LEFT_1=1071,22
PRIMER_RIGHT_1=1279,22
PRIMER_LEFT_1_TM=60.299
PRIMER_RIGHT_1_TM=60.215
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.5474
PRIMER_LEFT_2_PENALTY=0.332137
PRIMER_RIGHT_2_PENALTY=0.215225
PRIMER_LEFT_2_SEQUENCE=GAGATTGTGGAACAAGGGAAAA
PRIMER_RIGHT_2_SEQUENCE=AACAATATCCACTCATGGAGGC
PRIMER_LEFT_2=1103,22
PRIMER_RIGHT_2=1279,22
PRIMER_LEFT_2_TM=60.332
PRIMER_RIGHT_2_TM=60.215
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=0.5654
PRIMER_LEFT_3_PENALTY=0.237277
PRIMER_RIGHT_3_PENALTY=0.328094
PRIMER_LEFT_3_SEQUENCE=CTACACTCACTGCCGTGTTGAT
PRIMER_RIGHT_3_SEQUENCE=AAGCAATCAGTTTACAAAGCCC
PRIMER_LEFT_3=1018,22
PRIMER_RIGHT_3=1310,22
PRIMER_LEFT_3_TM=60.237
PRIMER_RIGHT_3_TM=59.672
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.6270
PRIMER_LEFT_4_PENALTY=0.298898
PRIMER_RIGHT_4_PENALTY=0.328094
PRIMER_LEFT_4_SEQUENCE=TGCTGAGTGAGTCAATGGTTTC
PRIMER_RIGHT_4_SEQUENCE=AAGCAATCAGTTTACAAAGCCC
PRIMER_LEFT_4=1071,22
PRIMER_RIGHT_4=1310,22
PRIMER_LEFT_4_TM=60.299
PRIMER_RIGHT_4_TM=59.672
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=240
=
PRIMER_SEQUENCE_ID=SSR_05381 Ps_contig_mira-and-tgicl-ass_franssen_37392
SEQUENCE=CCAAACCAACCTTAACTAACATAAGTCAACTTTTGTAATTCNTATATATATATATATAGTAGTAAGAAATCAAGCTCGGTAATATGGATTTTTCAAATTTGTACATCAACCACTTGCATTAGACTAAAATAGTATTAGTCCCTTTGAAATGAAATGTCACTAAAAAATGCTTGGAGGTTCTTATCTGAAGATCCACCTTTGCCGATAGCTTTATGACACATAACCTTAAGTTCTTTTGCTCTATCTCTAATTTTCTTTCCCTCTTGGCTTTCAAGATCCATAAACCTCTTAACAAGCACTTCTATATCTTCTTTTGCAAGAACTACTTCATTCTCTAACAATGGTGATACCTCTATCTTCCACCCATTTTTCCATTCATCAACAATTTGACTACTATTAGGAACTTGATCCAAGAAAAGTGGAAATGTCAAAATTGGTACCCCAACAAAAAGAGTTTCCAAAATTGAATTCCATCCACAATGGCTCCAAAATCCACCTATAGAAGAATGTGACAATACCCTTAGTTGATCACACCATGGTATCACCATGCCTTTATCTCCACACTTGTCTTTTAATCGCGAAGACTTCGCCGCGAGCAACATATAAGTATGGAATTCCAGTATTATTTAGCGCTTCAACAATTTCGTCCATTTGAGCATTGGATACCGAAAGGAAACTACCTAAAGAAATGTATAATACTGATTCATCTGGTTGAGAATCTAGCCATTTTATATAGTCGTGGCTATGATCTGTGTTTTTCAAAGTATTTTCTTCGAGTTCTAAATAGGGTATTGCAGGACCAATAGGGTATATGGGAAAGGAGAATATATGTTTAAGAGTATCAATTGTTTCAGCCTCTAATTCTTGAACCGTGGTTAACAGAAGATAATTTGCTTTGGATGCCATAGATATGCAATCTAATGCAAGCTGCATTGCTCGATCATCAGTTTTGTGAAGAACAGCTAGAAGATCTTTCACATAAAGTGAAGAAATTCCTGGAATGTTCTCTGTTTTTTCACCAAGTGTACCAATTGTTAAGTGTCGGTCACGTGAGAAGACATCAAGGTGATGTAACATAGAGTAAAACGACGCCGACATCGTCCAAAACGCAGCCACCGGAATATTCCTCCGATTAGCAACAGCAACAGGCCACCGCAGCTCAACGTCGCCAACAATCACATCCACCGGCGGTACAAGCTGATCAAGAAGCCGCTCAAACGGCTCTTCCATCTTCGTCATGACGGCTTCATAGAAACCTGGAAAATCACCTGCTTTCTCGCGCTCCGATGGAATCACGTTAGGAATAGTAGCGAAGCGGATGGCTTCCGGCTTTGGATCGGCGCCGATATAACCGAGCCACTCTTCGGTGATTACGAAGGTTANGAGATCTCGCTTGGTCTTCGTGATGCTAGGATCTTACAGAGCTTAGCAGTCGGGTTNATGTNTCCTCTTC
TARGET=43,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05382 Ps_contig_mira-and-tgicl-ass_franssen_37397
SEQUENCE=GGATGATGACGAATACATTTAGTATAAACTAGGATGACTTGAAAGAAAGGCCAAAAGTGGTGTTTGGCACTGAGCATTGCCCAACACAGTTGGTTCATTTTGAGAATATCAGATTCATTCATTGTATCCATCCAATAAGCAGGTTTTGGTATTATTAGAAGAACCTCAAGTTGACCAGCACAATCTGTAAGAAGATCTTAGGCTGTTTTAGATAAAAAATAATTGCCCATTATTGTTGGGTAACATATAGCAATTTGTGCGAGGGGAATTTCTTTCTTTCTGCTGTTGGGGGAAATTTCCCTTCCACCCCCTTCCNAACCCCCTAGTAGAAAAAAGATGATCTGTATAGTTCTTATTTGTTCCAACGTCAGCTTGTTGCAACTANGGTTGAGTATCGGATACTGATCTACTAGA
TARGET=269,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1826
PRIMER_LEFT_PENALTY=0.150698
PRIMER_RIGHT_PENALTY=0.031944
PRIMER_LEFT_SEQUENCE=GCCCATTATTGTTGGGTAACAT
PRIMER_RIGHT_SEQUENCE=ACCNTAGTTGCAACAAGCTGAC
PRIMER_LEFT=224,22
PRIMER_RIGHT=387,22
PRIMER_LEFT_TM=59.849
PRIMER_RIGHT_TM=60.032
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.50
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.3162
PRIMER_LEFT_1_PENALTY=0.150698
PRIMER_RIGHT_1_PENALTY=0.165541
PRIMER_LEFT_1_SEQUENCE=GCCCATTATTGTTGGGTAACAT
PRIMER_RIGHT_1_SEQUENCE=CAAGCTGACGTTGGAACAAATA
PRIMER_LEFT_1=224,22
PRIMER_RIGHT_1=374,22
PRIMER_LEFT_1_TM=59.849
PRIMER_RIGHT_1_TM=60.166
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=0.3209
PRIMER_LEFT_2_PENALTY=0.288917
PRIMER_RIGHT_2_PENALTY=0.031944
PRIMER_LEFT_2_SEQUENCE=GAAGAACCTCAAGTTGACCAGC
PRIMER_RIGHT_2_SEQUENCE=ACCNTAGTTGCAACAAGCTGAC
PRIMER_LEFT_2=157,22
PRIMER_RIGHT_2=387,22
PRIMER_LEFT_2_TM=60.289
PRIMER_RIGHT_2_TM=60.032
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.50
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.4545
PRIMER_LEFT_3_PENALTY=0.288917
PRIMER_RIGHT_3_PENALTY=0.165541
PRIMER_LEFT_3_SEQUENCE=GAAGAACCTCAAGTTGACCAGC
PRIMER_RIGHT_3_SEQUENCE=CAAGCTGACGTTGGAACAAATA
PRIMER_LEFT_3=157,22
PRIMER_RIGHT_3=374,22
PRIMER_LEFT_3_TM=60.289
PRIMER_RIGHT_3_TM=60.166
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.4624
PRIMER_LEFT_4_PENALTY=0.296849
PRIMER_RIGHT_4_PENALTY=0.165541
PRIMER_LEFT_4_SEQUENCE=CCAACACAGTTGGTTCATTTTG
PRIMER_RIGHT_4_SEQUENCE=CAAGCTGACGTTGGAACAAATA
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=374,22
PRIMER_LEFT_4_TM=60.297
PRIMER_RIGHT_4_TM=60.166
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_05383 Ps_contig_mira-and-tgicl-ass_franssen_37406
SEQUENCE=ATAGGGTGGATAAGAGACCGGGAACGGAACGGGAAGRGNTTTGGAGAATCCACTCAGCTGTGCCAGTGCAGCTGCAAGGGCATCTGTTCCTTTTTCTTCAATCAGTTTCTGTGCAGTTGGGGTGAAAAATGCAATAGACTTGGGATGGACTCCATTAAGTGTGACAACAACTTGCTCGGCAGATGCCTCCAAAACATCCTCCATAGACGGTACGTTAACAAATTCAAATTTGCATCCTACATCGCGCTCAAGGGATCGAACTGTTCTTCTCTGGTTACCTGTGTACATCAGAATGGCAGAACCTAGCTTTCCTGCACGGCCAGTACGACCAGAGCGATGTACAAAAGTCTCGGGATCATTGGGAAGTTCATAATGGATAATCAAATCAACATTGGGAATATCAAGTCCGCGAGATGCCACATCAGTGGCAACAAGCACTGTAAACTTTCCTTGTCTGAAACCATTCAATGTTCTCTCTCTCTGATGCTGAGATATATCGCCATGCAGTGCTTCAGAAGGGATATTATTTGTCAATGCCAATGATACTTCATCGGCATCTCTTTTTGTCTGTGTAAAAACTATAGTCTTCCCGCCCTTTGCGTAAACTGTAATAAGATCAGAAAGAATTGTTCTCTTAGAAGTGGTAGTGGCTGATATAGCATAAAGTTTTATCCCTTCGGCAAGCTTTTCTTCTTCATCACCAACCAAATCAATTGTCAACGGATTGTCCAGATATTTCCTGGCCAATTTCTTCACCCAACTAGGCATGGTTGCAGAAAAAAGCATGCTCTGTCTCTTAGCTGGAAGCTTTTCCAAAATGACTTCCACGGCCTCCTCAAACCCAACAGCAAGCATTTGATCAGCTTCATCGAGTACCAAATACTCAACTTCACTCAA
TARGET=472,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05384 Ps_contig_mira-and-tgicl-ass_franssen_37407
SEQUENCE=GAACGAAGCAAGAGAGAAAACAACTTTCATTTGCACTCTCTTCTTCTTTCTTCTTTTCATTATTCTCCCTTCAAAATAACTCACCTTAACAAACAAACACACGCCCGCCTATACCCTTTTCTTGTTCCAAACCAAATTCTAGTAGCAACAACAACAACTCTATATCACAAACATTGTTCTCCATTTGTAAAAACTGAAATGGAACTTGCTTTGAGCTTAGGTGACACACCAAAATTACCCTTCTCTTTTCTTGACAAACCCACCAAAGATCCACTACCACCAAGTTTCTTCTCCGAAGAAAAAACAAGTTCCTCAGATCCACCTATTCAACTTGACCTTCTTCCTTTCACTCCTGTTCTTAGATCTCATCACCCTTCTTCTCATCTTCCCATTCCATGGCTCACTCAACCATGTGGACCGGTGAGGGAGCTAGATGTAAACCGTTTTCCGGCGATGACGGAGGATCTCGACGACGGCGCGTCACTGTCATCACCCAGCAGTTCGGTGTCTCCGTTTCCTATGGATTTCAGTATGAGGAATAACAATGCTGAATATGGAGGAAGAAACAAGAGAGACAAAGAAGGTGACTCTGAAAGAGTGAGTGATGATGATGAAAACGGGTCAACTCGGAAGAAACTCAGACTCTCTAAAGATCAATCAGCTTTTCTAGAAGAAAGCTTCAAAGAACACACAACCTTAAATCCAAAGCAAAAACTTGCACTGGCAAAACAGTTGAATCTCAGTCCTCGTCAAGTTGAAGTTTGGTTTCAAAACAGAAGAGCCAGGACGAAGTTGAAGCAAACGGAAGTAGATTGTGAGTATTTGAAGAGATGTTGTGAGACATTAACAGAAGAGAATAGAAGATTACAAAAGGAACTTCAAGAGCTTAGAGCTTTGAAAACTACTCAACCATTCTACATGCAACTTCCAGCTACCACTCTCACTATGTGTCCTTCTTGTGAACGCGTGGCTACAAACCCTTGCAACAATCAAACCAACAAGACAAGAGGGTTGCCTTTCTCTAATGCCCAAGTCCAACAACAGGCCCAAGCCCAAGTTCAAGCCCATCAGATTCCTTCATGATAATATTATTTTCAAATGGTCACTTTTTTTATTTGTTTTTTAACATAATATATATGATAGATAATATCATAGAGGATGACAAGATTTAGTTTCACTAACCTATTTTGTGTATGTATATATAATTTAAGATTAGATTTAGACTCGTGCTATTTGTTGGTTTTTTTCTCTTATTTTCTATTACTATGAGTTGGTAGAATGATTCTTTTTTGTGATCNAATTAGAATTTACTTT
TARGET=88,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2449
PRIMER_LEFT_PENALTY=1.232712
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=GAACGAAGCAAGAGAGAAAACA
PRIMER_RIGHT_SEQUENCE=GGTGGGTTTGTCAAGAAAAGAG
PRIMER_LEFT=0,22
PRIMER_RIGHT=263,22
PRIMER_LEFT_TM=58.767
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=1.2515
PRIMER_LEFT_1_PENALTY=1.232712
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=GAACGAAGCAAGAGAGAAAACA
PRIMER_RIGHT_1_SEQUENCE=GGGTAATTTTGGTGTGTCACCT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=239,22
PRIMER_LEFT_1_TM=58.767
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=1.3344
PRIMER_LEFT_2_PENALTY=1.232712
PRIMER_RIGHT_2_PENALTY=0.101680
PRIMER_LEFT_2_SEQUENCE=GAACGAAGCAAGAGAGAAAACA
PRIMER_RIGHT_2_SEQUENCE=AGCTCAAAGCAAGTTCCATTTC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=216,22
PRIMER_LEFT_2_TM=58.767
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=217
PRIMER_PAIR_PENALTY_3=1.3901
PRIMER_LEFT_3_PENALTY=1.232712
PRIMER_RIGHT_3_PENALTY=0.157415
PRIMER_LEFT_3_SEQUENCE=GAACGAAGCAAGAGAGAAAACA
PRIMER_RIGHT_3_SEQUENCE=CGGAGAAGAAACTTGGTGGTAG
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=58.767
PRIMER_RIGHT_3_TM=60.157
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=1.5394
PRIMER_LEFT_4_PENALTY=1.232712
PRIMER_RIGHT_4_PENALTY=0.306698
PRIMER_LEFT_4_SEQUENCE=GAACGAAGCAAGAGAGAAAACA
PRIMER_RIGHT_4_SEQUENCE=TGTCACCTAAGCTCAAAGCAAG
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=225,22
PRIMER_LEFT_4_TM=58.767
PRIMER_RIGHT_4_TM=59.693
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_05385 Ps_contig_mira-and-tgicl-ass_franssen_37407
SEQUENCE=GAACGAAGCAAGAGAGAAAACAACTTTCATTTGCACTCTCTTCTTCTTTCTTCTTTTCATTATTCTCCCTTCAAAATAACTCACCTTAACAAACAAACACACGCCCGCCTATACCCTTTTCTTGTTCCAAACCAAATTCTAGTAGCAACAACAACAACTCTATATCACAAACATTGTTCTCCATTTGTAAAAACTGAAATGGAACTTGCTTTGAGCTTAGGTGACACACCAAAATTACCCTTCTCTTTTCTTGACAAACCCACCAAAGATCCACTACCACCAAGTTTCTTCTCCGAAGAAAAAACAAGTTCCTCAGATCCACCTATTCAACTTGACCTTCTTCCTTTCACTCCTGTTCTTAGATCTCATCACCCTTCTTCTCATCTTCCCATTCCATGGCTCACTCAACCATGTGGACCGGTGAGGGAGCTAGATGTAAACCGTTTTCCGGCGATGACGGAGGATCTCGACGACGGCGCGTCACTGTCATCACCCAGCAGTTCGGTGTCTCCGTTTCCTATGGATTTCAGTATGAGGAATAACAATGCTGAATATGGAGGAAGAAACAAGAGAGACAAAGAAGGTGACTCTGAAAGAGTGAGTGATGATGATGAAAACGGGTCAACTCGGAAGAAACTCAGACTCTCTAAAGATCAATCAGCTTTTCTAGAAGAAAGCTTCAAAGAACACACAACCTTAAATCCAAAGCAAAAACTTGCACTGGCAAAACAGTTGAATCTCAGTCCTCGTCAAGTTGAAGTTTGGTTTCAAAACAGAAGAGCCAGGACGAAGTTGAAGCAAACGGAAGTAGATTGTGAGTATTTGAAGAGATGTTGTGAGACATTAACAGAAGAGAATAGAAGATTACAAAAGGAACTTCAAGAGCTTAGAGCTTTGAAAACTACTCAACCATTCTACATGCAACTTCCAGCTACCACTCTCACTATGTGTCCTTCTTGTGAACGCGTGGCTACAAACCCTTGCAACAATCAAACCAACAAGACAAGAGGGTTGCCTTTCTCTAATGCCCAAGTCCAACAACAGGCCCAAGCCCAAGTTCAAGCCCATCAGATTCCTTCATGATAATATTATTTTCAAATGGTCACTTTTTTTATTTGTTTTTTAACATAATATATATGATAGATAATATCATAGAGGATGACAAGATTTAGTTTCACTAACCTATTTTGTGTATGTATATATAATTTAAGATTAGATTTAGACTCGTGCTATTTGTTGGTTTTTTTCTCTTATTTTCTATTACTATGAGTTGGTAGAATGATTCTTTTTTGTGATCNAATTAGAATTTACTTT
TARGET=146,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6291
PRIMER_LEFT_PENALTY=0.610296
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=TACCCTTTTCTTGTTCCAAACC
PRIMER_RIGHT_SEQUENCE=GGGTAATTTTGGTGTGTCACCT
PRIMER_LEFT=111,22
PRIMER_RIGHT=239,22
PRIMER_LEFT_TM=59.390
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=129
PRIMER_PAIR_PENALTY_1=0.7035
PRIMER_LEFT_1_PENALTY=0.684627
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=CCGCCTATACCCTTTTCTTGTT
PRIMER_RIGHT_1_SEQUENCE=GGGTAATTTTGGTGTGTCACCT
PRIMER_LEFT_1=104,22
PRIMER_RIGHT_1=239,22
PRIMER_LEFT_1_TM=60.685
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.7120
PRIMER_LEFT_2_PENALTY=0.610296
PRIMER_RIGHT_2_PENALTY=0.101680
PRIMER_LEFT_2_SEQUENCE=TACCCTTTTCTTGTTCCAAACC
PRIMER_RIGHT_2_SEQUENCE=AGCTCAAAGCAAGTTCCATTTC
PRIMER_LEFT_2=111,22
PRIMER_RIGHT_2=216,22
PRIMER_LEFT_2_TM=59.390
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=106
PRIMER_PAIR_PENALTY_3=0.7677
PRIMER_LEFT_3_PENALTY=0.610296
PRIMER_RIGHT_3_PENALTY=0.157415
PRIMER_LEFT_3_SEQUENCE=TACCCTTTTCTTGTTCCAAACC
PRIMER_RIGHT_3_SEQUENCE=CGGAGAAGAAACTTGGTGGTAG
PRIMER_LEFT_3=111,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=59.390
PRIMER_RIGHT_3_TM=60.157
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.7863
PRIMER_LEFT_4_PENALTY=0.684627
PRIMER_RIGHT_4_PENALTY=0.101680
PRIMER_LEFT_4_SEQUENCE=CCGCCTATACCCTTTTCTTGTT
PRIMER_RIGHT_4_SEQUENCE=AGCTCAAAGCAAGTTCCATTTC
PRIMER_LEFT_4=104,22
PRIMER_RIGHT_4=216,22
PRIMER_LEFT_4_TM=60.685
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=113
=
PRIMER_SEQUENCE_ID=SSR_05386 Ps_contig_mira-and-tgicl-ass_franssen_37407
SEQUENCE=GAACGAAGCAAGAGAGAAAACAACTTTCATTTGCACTCTCTTCTTCTTTCTTCTTTTCATTATTCTCCCTTCAAAATAACTCACCTTAACAAACAAACACACGCCCGCCTATACCCTTTTCTTGTTCCAAACCAAATTCTAGTAGCAACAACAACAACTCTATATCACAAACATTGTTCTCCATTTGTAAAAACTGAAATGGAACTTGCTTTGAGCTTAGGTGACACACCAAAATTACCCTTCTCTTTTCTTGACAAACCCACCAAAGATCCACTACCACCAAGTTTCTTCTCCGAAGAAAAAACAAGTTCCTCAGATCCACCTATTCAACTTGACCTTCTTCCTTTCACTCCTGTTCTTAGATCTCATCACCCTTCTTCTCATCTTCCCATTCCATGGCTCACTCAACCATGTGGACCGGTGAGGGAGCTAGATGTAAACCGTTTTCCGGCGATGACGGAGGATCTCGACGACGGCGCGTCACTGTCATCACCCAGCAGTTCGGTGTCTCCGTTTCCTATGGATTTCAGTATGAGGAATAACAATGCTGAATATGGAGGAAGAAACAAGAGAGACAAAGAAGGTGACTCTGAAAGAGTGAGTGATGATGATGAAAACGGGTCAACTCGGAAGAAACTCAGACTCTCTAAAGATCAATCAGCTTTTCTAGAAGAAAGCTTCAAAGAACACACAACCTTAAATCCAAAGCAAAAACTTGCACTGGCAAAACAGTTGAATCTCAGTCCTCGTCAAGTTGAAGTTTGGTTTCAAAACAGAAGAGCCAGGACGAAGTTGAAGCAAACGGAAGTAGATTGTGAGTATTTGAAGAGATGTTGTGAGACATTAACAGAAGAGAATAGAAGATTACAAAAGGAACTTCAAGAGCTTAGAGCTTTGAAAACTACTCAACCATTCTACATGCAACTTCCAGCTACCACTCTCACTATGTGTCCTTCTTGTGAACGCGTGGCTACAAACCCTTGCAACAATCAAACCAACAAGACAAGAGGGTTGCCTTTCTCTAATGCCCAAGTCCAACAACAGGCCCAAGCCCAAGTTCAAGCCCATCAGATTCCTTCATGATAATATTATTTTCAAATGGTCACTTTTTTTATTTGTTTTTTAACATAATATATATGATAGATAATATCATAGAGGATGACAAGATTTAGTTTCACTAACCTATTTTGTGTATGTATATATAATTTAAGATTAGATTTAGACTCGTGCTATTTGTTGGTTTTTTTCTCTTATTTTCTATTACTATGAGTTGGTAGAATGATTCTTTTTTGTGATCNAATTAGAATTTACTTT
TARGET=603,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1927
PRIMER_LEFT_PENALTY=0.164639
PRIMER_RIGHT_PENALTY=0.028056
PRIMER_LEFT_SEQUENCE=TGTCTCCGTTTCCTATGGATTT
PRIMER_RIGHT_SEQUENCE=TTCAACTTGACGAGGACTGAGA
PRIMER_LEFT=505,22
PRIMER_RIGHT=758,22
PRIMER_LEFT_TM=59.835
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.3215
PRIMER_LEFT_1_PENALTY=0.040447
PRIMER_RIGHT_1_PENALTY=0.281044
PRIMER_LEFT_1_SEQUENCE=CTCCGTTTCCTATGGATTTCAG
PRIMER_RIGHT_1_SEQUENCE=CTGAGTTTCTTCCGAGTTGACC
PRIMER_LEFT_1=508,22
PRIMER_RIGHT_1=640,22
PRIMER_LEFT_1_TM=59.960
PRIMER_RIGHT_1_TM=60.281
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=0.3241
PRIMER_LEFT_2_PENALTY=0.040447
PRIMER_RIGHT_2_PENALTY=0.283676
PRIMER_LEFT_2_SEQUENCE=CTCCGTTTCCTATGGATTTCAG
PRIMER_RIGHT_2_SEQUENCE=TGAGATTCAACTGTTTTGCCAG
PRIMER_LEFT_2=508,22
PRIMER_RIGHT_2=741,22
PRIMER_LEFT_2_TM=59.960
PRIMER_RIGHT_2_TM=60.284
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=234
PRIMER_PAIR_PENALTY_3=0.3241
PRIMER_LEFT_3_PENALTY=0.040447
PRIMER_RIGHT_3_PENALTY=0.283676
PRIMER_LEFT_3_SEQUENCE=CTCCGTTTCCTATGGATTTCAG
PRIMER_RIGHT_3_SEQUENCE=CTGAGATTCAACTGTTTTGCCA
PRIMER_LEFT_3=508,22
PRIMER_RIGHT_3=742,22
PRIMER_LEFT_3_TM=59.960
PRIMER_RIGHT_3_TM=60.284
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.3779
PRIMER_LEFT_4_PENALTY=0.096820
PRIMER_RIGHT_4_PENALTY=0.281044
PRIMER_LEFT_4_SEQUENCE=TCTTCTCATCTTCCCATTCCAT
PRIMER_RIGHT_4_SEQUENCE=CTGAGTTTCTTCCGAGTTGACC
PRIMER_LEFT_4=375,22
PRIMER_RIGHT_4=640,22
PRIMER_LEFT_4_TM=59.903
PRIMER_RIGHT_4_TM=60.281
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_05387 Ps_contig_mira-and-tgicl-ass_franssen_37410
SEQUENCE=GGTCGGGGGATCCATCATCACATCATTCTTGACGGTAAACATCCTTCCCCGCTAAGATTCCTTTTCTCTTATTATCACCCACCTCTCCTTTTCTTTCTCACCCCCACCTTCTCTTCTCATCACCTAGCTATAGAGAATTCTCTTGAATTCACGTTTCCTCAAAGAAGAAAATCAACAGATCAGATCCAATCCAGATTCATCGATGGCGGAAGAACAACCGTGTCAAGCTCCTGAAGGTCACCGTCTTTGCGCCAACAACTGCGGCATCCTCGGTAATCCGGCAACCATGAACCTCTGCTCGAAATGCTACGGTGATATCCGATTGAAGAATCAACAAGACACTTCCACCAAATCCACCATCGAAAACGCTCTCTCATCATATTCCTCCGTCGCATCCGTCACCACACCGCCTCTTTTGACGTCAGAGCCAACCGTTGTTTCGACGCCGATTTTAACAGAGATCACGTCGAGTTCTGTAACAACCGGTTCGGTTGGTTCTGTTTCTATTACGGTTCCACCGAACCGATGTTTGAGTTGTAGAAAGCGCGTGGGGTTAACCGGGTTTAAGTGTAAGTGCGGGTCGATGTTTTGTGGGACCCATAGGTACCCGGAGAGACACGGGTGTGGGTTTGATTTTAAGACGGTGGGGAGAGAGGAAATAGCGCGAGCGAACCCTTTGATAAGAGCGGAGAAACTACAGAGGATTTGATTCGATCTTAGCCGTTGGATGAGATGTGGGACGGTGTGTTTGTATTAGATGATTGCGGCCGTCCACGTGGACCGCCAGGAAATGATATCACATTGATATATGATTATTATTATTATATGTAAAATGTATGATTTTTCTGATAATGATTTTATGATTTAAGATTAACTT
TARGET=812,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05388 Ps_contig_mira-and-tgicl-ass_franssen_37413
SEQUENCE=CAGGGAATAATCGGAGTTTATTGAGTAATTATTTGTACAGTCCAGATATCATTACACTACTGTTTGGTAATATTTCACACATTTCAGCATCTTAAATAGGTTATATATCCAGATTTATACACAAAACAAAAACAAAAAATGAGAAGCACCCTGTTTAAAAGTAGACCTTTTGGGAGGTAGCAACTTCACAATCATAATTCATGAGAACTTGCACCAGAGAACCAGCATCCTTGATATTAATCTTCTATGTTCTTCATATACTCCTTTGAAAGTTTAGTTTCCATTATCAACTCTTGTTCTTTGAGTAGTGAATAACCTGACTCAAGTAGAGCTTCGGTTGTTTGCTCTGGAGGAATAAATACAGGAAGATTCTTGTCCTTTCTTCTTTTGAGAAAATTGGATGCAAAATTTTCAGCACCTCCAATATCATCAATCCACTTTAAAATAGATTTGTCATATGTACGCGATCTTAGAGCTCCATAAAAGTCCAATGCTTGATTAGGAAAAGTGTTCACAATTTTTTCAACTTCATCCCTAGATATGCCATCTTTCTCATACATTCTTTGAACAATATTCAGTATATCTTCTCGGTTAGGCTGCCAGTAAAATTTATCCATCCTTCCATCGCGAATCAGTGGAGCATAAATAGTTGATAGATCATTCCCTGTTACAATAATTGGAATTCTGTTTGTGACATCTGATTCTCGCCAATCTTGACCAACACTAACTCTTTTAGGATTGTCACATAGATTCATAAGAGTCCCAACAACAATTTGGTTGTTGACTGTCATTTGAGTATTCCCGAATCTACCAAGGCCAGCATCAATGTCATTGATCATTAAACAGCTCATTTTTCCTTGATTCTGGACCACTTGAGATGCTGTTCTATAGCGCTCACGAATCAATCTTCCCGGCTCTCCCGCATTTTCTGATTCTAGTTCCCCTGCGGACATAATTACAGGTTCAACTCCCATAGCCCGAAAGATAAGTTCTGTTTGAAATGTTTTCCCTTGCCCTTTACCTCCCCAAATACCTAGAATCAAAGGAACTTTAGCCTTGAGGATATGAGTAATGTAATTCTTCACAATGTGGCATGCAACTTTGTCCATGAAAAGAGGCGCAATGTAATAATCTCCTTGAAGATATTCAAAGGATCTGGTAACTTTCTGACGATAATCAAAAACAATGTCGGAGTCTTTGCCGAGAAAATCACCGACGAAAAGATCGTCGATGATTCCGGCACGCTGACCAACGGCGATGTTCATGTTATCAGCTTCTTTGCCGAGTCTGTAGAGATAATCTTTTCCCTCAGAATCCTTGGTTTCCCATGAAGACTGCTTTGATAGCCTCTTCTTGTTCTTGTTGTTGTTGGTATCTTCATTGTCACGATCGCAGCAGCGAACGCAGAATTGTTGGGGTTTGAATTTGAATGAAAGGTTGGGAGTTGGAAGACATAACGGTAGGAATAGTAGCTTCGCCATTTCATCCCATAACTTGTTTCCATTGCTCGCCACATNACAAAAACATAACATCAGCATCTTCCACTCATTTTCATT
TARGET=1360,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1627
PRIMER_LEFT_PENALTY=0.080887
PRIMER_RIGHT_PENALTY=0.081801
PRIMER_LEFT_SEQUENCE=GTTATCAGCTTCTTTGCCGAGT
PRIMER_RIGHT_SEQUENCE=ACAAGTTATGGGATGAAATGGC
PRIMER_LEFT=1265,22
PRIMER_RIGHT=1497,22
PRIMER_LEFT_TM=59.919
PRIMER_RIGHT_TM=60.082
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.2169
PRIMER_LEFT_1_PENALTY=0.080887
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=GTTATCAGCTTCTTTGCCGAGT
PRIMER_RIGHT_1_SEQUENCE=TATGTCTTCCAACTCCCAACCT
PRIMER_LEFT_1=1265,22
PRIMER_RIGHT_1=1455,22
PRIMER_LEFT_1_TM=59.919
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.2873
PRIMER_LEFT_2_PENALTY=0.205455
PRIMER_RIGHT_2_PENALTY=0.081801
PRIMER_LEFT_2_SEQUENCE=AGCTTCTTTGCCGAGTCTGTAG
PRIMER_RIGHT_2_SEQUENCE=ACAAGTTATGGGATGAAATGGC
PRIMER_LEFT_2=1271,22
PRIMER_RIGHT_2=1497,22
PRIMER_LEFT_2_TM=60.205
PRIMER_RIGHT_2_TM=60.082
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=0.3019
PRIMER_LEFT_3_PENALTY=0.080887
PRIMER_RIGHT_3_PENALTY=0.220992
PRIMER_LEFT_3_SEQUENCE=GTTATCAGCTTCTTTGCCGAGT
PRIMER_RIGHT_3_SEQUENCE=TTATGTCTTCCAACTCCCAACC
PRIMER_LEFT_3=1265,22
PRIMER_RIGHT_3=1456,22
PRIMER_LEFT_3_TM=59.919
PRIMER_RIGHT_3_TM=60.221
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=0.3027
PRIMER_LEFT_4_PENALTY=0.080887
PRIMER_RIGHT_4_PENALTY=0.221785
PRIMER_LEFT_4_SEQUENCE=GTTATCAGCTTCTTTGCCGAGT
PRIMER_RIGHT_4_SEQUENCE=TGGCGAAGCTACTATTCCTACC
PRIMER_LEFT_4=1265,22
PRIMER_RIGHT_4=1479,22
PRIMER_LEFT_4_TM=59.919
PRIMER_RIGHT_4_TM=59.778
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_05389 Ps_contig_mira-and-tgicl-ass_franssen_37421
SEQUENCE=ATTCATTTGTTAAAAATAATTCTCTCTCTTCAAGAATAACCAGAGTTGCTCTGCAACTCCTTTCATCTTCTCCGTTCCCAACCTACCGGAGAAGGCGGTGGTCGCCGGCCAACGGCGGCGGCGCCACCTTCTTCTCCGGCGAATCACTTCCCCAAAAACCAAAACCTAAACACTAATCTTCCTATTTCAACCTCGCGGTTACGGATCCGGCCATATTTTGCCCCAAAAAAAACCTTAAACCAACAGATCTAAACTCACCCACACCAAACAAACAAAAGTTAGGCAGAACAAACAAAGCTTTTAGGTTAACGAAATGAGATCAAGATGGATAAATAGGAAGCTTAGAAGAAAGGAACCAATCAATCGTACTCGAAGCTTC
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8314
PRIMER_LEFT_PENALTY=0.776469
PRIMER_RIGHT_PENALTY=0.054900
PRIMER_LEFT_SEQUENCE=CCCCAAAAACCAAAACCTAAAC
PRIMER_RIGHT_SEQUENCE=CGATTGATTGGTTCCTTTCTTC
PRIMER_LEFT=149,22
PRIMER_RIGHT=365,22
PRIMER_LEFT_TM=60.776
PRIMER_RIGHT_TM=59.945
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.9574
PRIMER_LEFT_1_PENALTY=0.902519
PRIMER_RIGHT_1_PENALTY=0.054900
PRIMER_LEFT_1_SEQUENCE=CCAACAGATCTAAACTCACCCA
PRIMER_RIGHT_1_SEQUENCE=CGATTGATTGGTTCCTTTCTTC
PRIMER_LEFT_1=239,22
PRIMER_RIGHT_1=365,22
PRIMER_LEFT_1_TM=59.097
PRIMER_RIGHT_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=1.1404
PRIMER_LEFT_2_PENALTY=1.085505
PRIMER_RIGHT_2_PENALTY=0.054900
PRIMER_LEFT_2_SEQUENCE=CCAACAGATCTAAACTCACCCAC
PRIMER_RIGHT_2_SEQUENCE=CGATTGATTGGTTCCTTTCTTC
PRIMER_LEFT_2=239,23
PRIMER_RIGHT_2=365,22
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=59.945
PRIMER_LEFT_2_GC_PERCENT=47.826
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=127
PRIMER_PAIR_PENALTY_3=1.1404
PRIMER_LEFT_3_PENALTY=1.085505
PRIMER_RIGHT_3_PENALTY=0.054900
PRIMER_LEFT_3_SEQUENCE=ACCAACAGATCTAAACTCACCCA
PRIMER_RIGHT_3_SEQUENCE=CGATTGATTGGTTCCTTTCTTC
PRIMER_LEFT_3=238,23
PRIMER_RIGHT_3=365,22
PRIMER_LEFT_3_TM=59.914
PRIMER_RIGHT_3_TM=59.945
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=128
PRIMER_PAIR_PENALTY_4=1.1490
PRIMER_LEFT_4_PENALTY=0.776469
PRIMER_RIGHT_4_PENALTY=0.372557
PRIMER_LEFT_4_SEQUENCE=CCCCAAAAACCAAAACCTAAAC
PRIMER_RIGHT_4_SEQUENCE=CGAGTACGATTGATTGGTTCCT
PRIMER_LEFT_4=149,22
PRIMER_RIGHT_4=371,22
PRIMER_LEFT_4_TM=60.776
PRIMER_RIGHT_4_TM=60.373
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_05390 Ps_contig_mira-and-tgicl-ass_franssen_37423
SEQUENCE=TACTATAGTCAAGTTTCTACCATCAAGTGTGGTGAGATTCACTGTGCAGCCGGATAATGCTTCTGCAAGAGAGATCTTCTGAGTGGCAACCAGATCATTTCCTTCACGAGTGAAGACGCTGTGTGGTTTTTCTATCAATGACAAACACAATATCAGCGGGTATTGAATTTGGATGTTCGTTTCCCTTCTCCGGGAAGGTGATCTTTGTGCCCTTCTTCCAACCAGGTTTGATATTAATAGTCAAGATCTCGTCCAACTGCATGAGTTTGCCATGAACATCAATAACTTCTCTGGTGATCTTCATCTTCCTTGTGGTCCCTTTATATATATCCTCAAGAGCACACGGCAATTTGTTCTCAATAGGCGGTGCCTTACGAGGAGCACTTTGATGATGCACATGAGCTCCACTTCCTCCTCCATCCCCAAAGGATCCAAACATATTCTCAACAAACATACCATTCGGAAGCCTTACTCCCATGCCACCAGAACCACTAGCACCTCTTCCTATCCCTCCCATGCCACCAAAGGGACTTGAAACCCCAAAAAAACTCAGCAAAAATAATCATCAGCATTCCGCTGATTGAACCGAAACGAAGCCGGTATATCCGCGGGAGAATAATAAGTGGGTCCACCACCACCAAAAGCTTCCGATGGTGGTGGCATTTGACCCTTCAAACCATCTTCCCCATATTGATCATAGATAGCCCTCTTCTGAGAATCACTCAGAACATCATAAGCTTCAGAAATTTGCTTGAATTTGGCTTCAGCTTCTTTCTTGTTGTTAGGGTTTTTGTCGAGGGTCNCCTATTTCATAGCGTAGTTTTCTGTAAGTCTTTCTACAAATCATCCTTCTTTAGCCGTTCCTATCAACCTGAAGAA
TARGET=629,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2629
PRIMER_LEFT_PENALTY=0.136083
PRIMER_RIGHT_PENALTY=0.126860
PRIMER_LEFT_SEQUENCE=AACATACCATTCGGAAGCCTTA
PRIMER_RIGHT_SEQUENCE=ATGATCAATATGGGGAAGATGG
PRIMER_LEFT=449,22
PRIMER_RIGHT=697,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=59.873
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.2660
PRIMER_LEFT_1_PENALTY=0.139129
PRIMER_RIGHT_1_PENALTY=0.126860
PRIMER_LEFT_1_SEQUENCE=TCTCAACAAACATACCATTCGG
PRIMER_RIGHT_1_SEQUENCE=ATGATCAATATGGGGAAGATGG
PRIMER_LEFT_1=441,22
PRIMER_RIGHT_1=697,22
PRIMER_LEFT_1_TM=59.861
PRIMER_RIGHT_1_TM=59.873
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.2660
PRIMER_LEFT_2_PENALTY=0.139129
PRIMER_RIGHT_2_PENALTY=0.126860
PRIMER_LEFT_2_SEQUENCE=CTCAACAAACATACCATTCGGA
PRIMER_RIGHT_2_SEQUENCE=ATGATCAATATGGGGAAGATGG
PRIMER_LEFT_2=442,22
PRIMER_RIGHT_2=697,22
PRIMER_LEFT_2_TM=59.861
PRIMER_RIGHT_2_TM=59.873
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.3231
PRIMER_LEFT_3_PENALTY=0.301926
PRIMER_RIGHT_3_PENALTY=0.021195
PRIMER_LEFT_3_SEQUENCE=CAGAACCACTAGCACCTCTTCC
PRIMER_RIGHT_3_SEQUENCE=AGAAGCTGAAGCCAAATTCAAG
PRIMER_LEFT_3=483,22
PRIMER_RIGHT_3=771,22
PRIMER_LEFT_3_TM=60.302
PRIMER_RIGHT_3_TM=60.021
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=0.3231
PRIMER_LEFT_4_PENALTY=0.301926
PRIMER_RIGHT_4_PENALTY=0.021195
PRIMER_LEFT_4_SEQUENCE=CCAGAACCACTAGCACCTCTTC
PRIMER_RIGHT_4_SEQUENCE=AGAAGCTGAAGCCAAATTCAAG
PRIMER_LEFT_4=482,22
PRIMER_RIGHT_4=771,22
PRIMER_LEFT_4_TM=60.302
PRIMER_RIGHT_4_TM=60.021
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_05391 Ps_contig_mira-and-tgicl-ass_franssen_37434
SEQUENCE=AATAACCCCAACCTATTCTTTTACCCATTTCATTTCAGTTTCTTTATCAACCAAAACTAAACCGAACCACTTCATCATCATCATCGTCGTCGTCGTCTTTATTACACCAATCATACACCACGCAGATCTTCTTCCCTCTGGTTTTTGTAATCGGTTAGCAGAAGAATTTGATGCTGAGTTATTATTATAAACAGAAAAGTTGAGGTGGGGGTTGGAGTATATGGATAGTTTCTGTTGCTTCAGCTCTGTCAGTCAGGCGGTTGGAGGGCGTTCGTCAAGCAACTCTGGCAAAGGTAAAGGCAGTCAGTCGCCGGTCAAATATGGATTTAGCCTTGTTAAAGGGAAAGCCAACCATCCAATGGAGGACTATCACGTAGCGAAATTTGTCCATTTCAAAGGACAAGAGCTAGGACTATTTGCCATATACGATGGACACCTCGGAGACAGTGTACCGTCATATCTGCAAAAGTATCTCTTTTCTAATATCTTGAAGGAAGATGACTTCTGGAACGATCCATTCATGTCGATTTCTAAAGCTTACGAGTCAACAGATCAGGCGATTCTTTCTCACAGTCCTGATTTGGGGCGAGGAGGATCAACCGCCGTGACTGCAATTCTCATAAATAATCAGAAACTCTGGGTCGCCAATGTTGGAGATTCACGAGCAGTTCTGTCAAGGGAAGGTGTAGCTGTACAGATGACTACCGATCATGAACCTAATACGGAACGTGGCAGTATTGAGAATAGAGGCGGCTTTGTCTCAAACATGCCAGGAGATGTTGCAAGAGTGAATGGGCAGCTTGCGGTTTCTCGAGCATTTGGAGACAAAAACCTCAAAACGCACTTGCGATCTGACCCCGACATTCAGTATGCTGATGTTAACCAAGATACCGAGCTTTTGATTCTCGCTAGTGATGGTCTATGGAAGGTAATGGCGAATCAAGAAGCGGTCGATATCGCGAAAAGAATAAAGGATCCACAAAAGGCAGCTAAACAACTAGCTACCGAGGCATTGAACAGAGACAGTAAGGATGATATTTCCTGCGTTGTAGTTCGTTTCAAGGGATGAAAAAGGGAACATACACACATACATATGTAAAAAAAAAGTTTATAAAATTGAGGTTCTTGAGTTTAGGTGGTGTGAAATTTAGAATTTATTGATTTCTTGGTTGTGATATAGATTCATACATGATAAATTTTCTTTGTAATATGCTTTATGTGATGGAGTAGTGTGATGATGGATGGCCACAGTTCATACATTATTGAACATGGGATTATTTATTTAAAAAATGAGTGAGTGTATAGAAAAAATT
TARGET=72,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3013
PRIMER_LEFT_PENALTY=0.249232
PRIMER_RIGHT_PENALTY=0.052069
PRIMER_LEFT_SEQUENCE=TCAACCAAAACTAAACCGAACC
PRIMER_RIGHT_SEQUENCE=CTTTACCTTTGCCAGAGTTGCT
PRIMER_LEFT=46,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=60.249
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.4464
PRIMER_LEFT_1_PENALTY=0.249232
PRIMER_RIGHT_1_PENALTY=0.197140
PRIMER_LEFT_1_SEQUENCE=TCAACCAAAACTAAACCGAACC
PRIMER_RIGHT_1_SEQUENCE=GACAGAGCTGAAGCAACAGAAA
PRIMER_LEFT_1=46,22
PRIMER_RIGHT_1=249,22
PRIMER_LEFT_1_TM=60.249
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.5157
PRIMER_LEFT_2_PENALTY=0.493430
PRIMER_RIGHT_2_PENALTY=0.022302
PRIMER_LEFT_2_SEQUENCE=ACCCCAACCTATTCTTTTACCC
PRIMER_RIGHT_2_SEQUENCE=AGATCTGCGTGGTGTATGATTG
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=128,22
PRIMER_LEFT_2_TM=59.507
PRIMER_RIGHT_2_TM=60.022
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.5216
PRIMER_LEFT_3_PENALTY=0.249232
PRIMER_RIGHT_3_PENALTY=0.272373
PRIMER_LEFT_3_SEQUENCE=TCAACCAAAACTAAACCGAACC
PRIMER_RIGHT_3_SEQUENCE=TATCCATATACTCCAACCCCCA
PRIMER_LEFT_3=46,22
PRIMER_RIGHT_3=226,22
PRIMER_LEFT_3_TM=60.249
PRIMER_RIGHT_3_TM=60.272
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.5377
PRIMER_LEFT_4_PENALTY=0.249232
PRIMER_RIGHT_4_PENALTY=0.288468
PRIMER_LEFT_4_SEQUENCE=TCAACCAAAACTAAACCGAACC
PRIMER_RIGHT_4_SEQUENCE=TTTACCTTTGCCAGAGTTGCTT
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=60.288
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_05392 Ps_contig_mira-and-tgicl-ass_franssen_37434
SEQUENCE=AATAACCCCAACCTATTCTTTTACCCATTTCATTTCAGTTTCTTTATCAACCAAAACTAAACCGAACCACTTCATCATCATCATCGTCGTCGTCGTCTTTATTACACCAATCATACACCACGCAGATCTTCTTCCCTCTGGTTTTTGTAATCGGTTAGCAGAAGAATTTGATGCTGAGTTATTATTATAAACAGAAAAGTTGAGGTGGGGGTTGGAGTATATGGATAGTTTCTGTTGCTTCAGCTCTGTCAGTCAGGCGGTTGGAGGGCGTTCGTCAAGCAACTCTGGCAAAGGTAAAGGCAGTCAGTCGCCGGTCAAATATGGATTTAGCCTTGTTAAAGGGAAAGCCAACCATCCAATGGAGGACTATCACGTAGCGAAATTTGTCCATTTCAAAGGACAAGAGCTAGGACTATTTGCCATATACGATGGACACCTCGGAGACAGTGTACCGTCATATCTGCAAAAGTATCTCTTTTCTAATATCTTGAAGGAAGATGACTTCTGGAACGATCCATTCATGTCGATTTCTAAAGCTTACGAGTCAACAGATCAGGCGATTCTTTCTCACAGTCCTGATTTGGGGCGAGGAGGATCAACCGCCGTGACTGCAATTCTCATAAATAATCAGAAACTCTGGGTCGCCAATGTTGGAGATTCACGAGCAGTTCTGTCAAGGGAAGGTGTAGCTGTACAGATGACTACCGATCATGAACCTAATACGGAACGTGGCAGTATTGAGAATAGAGGCGGCTTTGTCTCAAACATGCCAGGAGATGTTGCAAGAGTGAATGGGCAGCTTGCGGTTTCTCGAGCATTTGGAGACAAAAACCTCAAAACGCACTTGCGATCTGACCCCGACATTCAGTATGCTGATGTTAACCAAGATACCGAGCTTTTGATTCTCGCTAGTGATGGTCTATGGAAGGTAATGGCGAATCAAGAAGCGGTCGATATCGCGAAAAGAATAAAGGATCCACAAAAGGCAGCTAAACAACTAGCTACCGAGGCATTGAACAGAGACAGTAAGGATGATATTTCCTGCGTTGTAGTTCGTTTCAAGGGATGAAAAAGGGAACATACACACATACATATGTAAAAAAAAAGTTTATAAAATTGAGGTTCTTGAGTTTAGGTGGTGTGAAATTTAGAATTTATTGATTTCTTGGTTGTGATATAGATTCATACATGATAAATTTTCTTTGTAATATGCTTTATGTGATGGAGTAGTGTGATGATGGATGGCCACAGTTCATACATTATTGAACATGGGATTATTTATTTAAAAAATGAGTGAGTGTATAGAAAAAATT
TARGET=84,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3013
PRIMER_LEFT_PENALTY=0.249232
PRIMER_RIGHT_PENALTY=0.052069
PRIMER_LEFT_SEQUENCE=TCAACCAAAACTAAACCGAACC
PRIMER_RIGHT_SEQUENCE=CTTTACCTTTGCCAGAGTTGCT
PRIMER_LEFT=46,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=60.249
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.4464
PRIMER_LEFT_1_PENALTY=0.249232
PRIMER_RIGHT_1_PENALTY=0.197140
PRIMER_LEFT_1_SEQUENCE=TCAACCAAAACTAAACCGAACC
PRIMER_RIGHT_1_SEQUENCE=GACAGAGCTGAAGCAACAGAAA
PRIMER_LEFT_1=46,22
PRIMER_RIGHT_1=249,22
PRIMER_LEFT_1_TM=60.249
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.5157
PRIMER_LEFT_2_PENALTY=0.493430
PRIMER_RIGHT_2_PENALTY=0.022302
PRIMER_LEFT_2_SEQUENCE=ACCCCAACCTATTCTTTTACCC
PRIMER_RIGHT_2_SEQUENCE=AGATCTGCGTGGTGTATGATTG
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=128,22
PRIMER_LEFT_2_TM=59.507
PRIMER_RIGHT_2_TM=60.022
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.5216
PRIMER_LEFT_3_PENALTY=0.249232
PRIMER_RIGHT_3_PENALTY=0.272373
PRIMER_LEFT_3_SEQUENCE=TCAACCAAAACTAAACCGAACC
PRIMER_RIGHT_3_SEQUENCE=TATCCATATACTCCAACCCCCA
PRIMER_LEFT_3=46,22
PRIMER_RIGHT_3=226,22
PRIMER_LEFT_3_TM=60.249
PRIMER_RIGHT_3_TM=60.272
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=11.2000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.5377
PRIMER_LEFT_4_PENALTY=0.249232
PRIMER_RIGHT_4_PENALTY=0.288468
PRIMER_LEFT_4_SEQUENCE=TCAACCAAAACTAAACCGAACC
PRIMER_RIGHT_4_SEQUENCE=TTTACCTTTGCCAGAGTTGCTT
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=60.288
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_05393 Ps_contig_mira-and-tgicl-ass_franssen_37436
SEQUENCE=GCGTTGCTCAATCTCGGAATCGGAAACGATGCAAATAAAGCAGCCATTGTTAAAGTTGGTTCTGTTCATAAGATGCTTAAGCTTGTTGAATCTTCAGATGATACTGATTCTGCGGTTTCTGAAGCGGTTGTTGCAAATTTCCTTGGATTGAGTGCTTTGGATTCGAATAAACCGATAATCGGGTCGTCTGCTGCTATACCGTTTTTAGTTAGAACCCTTCGGAGTTTAGATAAGAAAGATAGTAGCAATCAAGCTAAGCAGGATGTTCTTAGAGCACTTTACAATCTTTCAATCTTTCCAGCAAATATTCTATTCATTTTGGAAACTGATTTGGTTCCTTTTCTGATAAACTCGATTGGCGATATGGAAGTAACGGAAAGAAACCTCTCTATCCTTAGCAATTTGGTGTCAAGTAGAGCAGGTAGAAAAGCGATAAGTGCTGTCCCTGATGTGTTTCCTATATTGGTTGATGTATTGAATTGGACTGATTCGCCTGAATGCCAGGAAAAGGTTTCGTATGTTTTGATGGTTATGTCGCATAAATCTTATGGTGATAAGCAGGCCATGATTGAGGCAGGGGTTGTGTCGTCCCTTCTTGAGTTGTCGCTTATTGGTACTACTCTGTCTCAGAATCGGGCTTCAAGGCTATTGGAAAGTTTGAGAATAGATAAAGGGAAACAAGTTTCAGGTAACTATGGCGGAGGAAATTTAGGCACAGCAGTCTCTGCCCCAATTTGCGGAACTTCATCATCATGTGCAAAACCAGATGGAGGAGGAGGAGGAAAAGAGTGTTCGGAAGAAGACGAAGATATGATGAGCGACGAAAAGAAAGCAGTGAAGCAATTAGTCCAATTGAGTTTGCAAAACAACATGAGAAAAATCGTCAAAAGGGCCAACTTGCCTCAAGATATCGTTCCATCAGATCATTTTAAGTCACTCACATCAAGTTCAACTTCGAAGAGTTTACCATTCTGAAGAATTTCAGATGTGCAGTGAGTCTAGGTTGGGGAGAATGAACCATAATGTATGTTAGTCTTTCTTTCATAATTTTATTGAGCTTTTAACCTTGTGTATTGTATGCAGTAGTCAACTTGTTTTACTAAAAGT
TARGET=769,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4011
PRIMER_LEFT_PENALTY=0.021195
PRIMER_RIGHT_PENALTY=0.379939
PRIMER_LEFT_SEQUENCE=GTTTCAGGTAACTATGGCGGAG
PRIMER_RIGHT_SEQUENCE=GGAACGATATCTTGAGGCAAGT
PRIMER_LEFT=681,22
PRIMER_RIGHT=916,22
PRIMER_LEFT_TM=60.021
PRIMER_RIGHT_TM=59.620
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.4011
PRIMER_LEFT_1_PENALTY=0.021195
PRIMER_RIGHT_1_PENALTY=0.379939
PRIMER_LEFT_1_SEQUENCE=AGTTTCAGGTAACTATGGCGGA
PRIMER_RIGHT_1_SEQUENCE=GGAACGATATCTTGAGGCAAGT
PRIMER_LEFT_1=680,22
PRIMER_RIGHT_1=916,22
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=59.620
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=0.4364
PRIMER_LEFT_2_PENALTY=0.021195
PRIMER_RIGHT_2_PENALTY=0.415247
PRIMER_LEFT_2_SEQUENCE=GTTTCAGGTAACTATGGCGGAG
PRIMER_RIGHT_2_SEQUENCE=TTCGTCTTCTTCCGAACACTCT
PRIMER_LEFT_2=681,22
PRIMER_RIGHT_2=806,22
PRIMER_LEFT_2_TM=60.021
PRIMER_RIGHT_2_TM=60.415
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.4364
PRIMER_LEFT_3_PENALTY=0.021195
PRIMER_RIGHT_3_PENALTY=0.415247
PRIMER_LEFT_3_SEQUENCE=GTTTCAGGTAACTATGGCGGAG
PRIMER_RIGHT_3_SEQUENCE=CTTCGTCTTCTTCCGAACACTC
PRIMER_LEFT_3=681,22
PRIMER_RIGHT_3=807,22
PRIMER_LEFT_3_TM=60.021
PRIMER_RIGHT_3_TM=60.415
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=0.4364
PRIMER_LEFT_4_PENALTY=0.021195
PRIMER_RIGHT_4_PENALTY=0.415247
PRIMER_LEFT_4_SEQUENCE=AGTTTCAGGTAACTATGGCGGA
PRIMER_RIGHT_4_SEQUENCE=CTTCGTCTTCTTCCGAACACTC
PRIMER_LEFT_4=680,22
PRIMER_RIGHT_4=807,22
PRIMER_LEFT_4_TM=60.021
PRIMER_RIGHT_4_TM=60.415
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_05394 Ps_contig_mira-and-tgicl-ass_franssen_37437
SEQUENCE=ACTTTTTATTGAAACTCTAAGATGATTTAGTACTAGATTCTTAAANTAATAGCTATGATTTCATGCTTAGCATGATGAATCATGAGAAGAAGTGCTAAAAGCAAGGATTGCCAGTGATTATTCTCCTTCACATACTTCCAGTTTTACATTGAAAAGGAAAAAAGAAGAAGAAAAGAATATACAAGGCTTCCACCATTATTCTCTATAAAAGTCCAACAACACAGCTCAAATGCAATCTGTTTTCCAATCCTCTCAAGTGATGACCCAATAACATACACCAGAAAAGCAACTTCGCTAGGTAATCGCGAGAAGTGGCTACTGTATCACTAAACTCATCACTGTCGTCGGGGTGATAGTCTACGTTTCCTTCTTCTGACTGTTCCATAAAGTTAGAGCAAGCTTCGTCGACAAGGAATCTTCTCATAATGTTTTGAACAAGTTGGTGAATTATATCCTCCACTTCAACGGACGAAGGTCGGGACATTTCATTCACCCTATCAGGATCAAAAGAGCGAAGATATTCCAGTAAATTATTCCGAATTCCTCTGTCATGTTCTAATTGCATCATTTCTTGCTTCTGCGCATTCAGCTCCTCCTTTGTGTTTGACAACTCAGACTGCAACTGTTGGATGTATTTCAGAGCCTGGGGCGGCAAATCTCGAAAGACTCCCGTGCCACTACTGCTGTTGCTGCAAGTTTCCGAATCATTGAATCCTGAATCAGAAACAACCTTCTTATTTTCCCCATGTTCTTCTCCTCCACCCTTGACCTCCAAACTCTCCAAAGGCGGTTCACACTCCGACACTCCATCCGCACAAGACATTTCCAGATTCCTCGTCAAGGACATCCTGTACTCCGCATTCCACAGCGTGTACCCTGTGATGATGGAAGAAACAAGCAACCGATGAAGTGGCTGAATAGAAAGACTAACAGTAACAGAGAAATGATCAGAAGGTAACAAACCAAGCATAGTGGAAATAGTTTGTTTCATAGAATCTTTAGCAGCAGTAGAAACACCTATAGAGATAACGGAGTTATCGAGGGGTTTGATACGGCGAAGCATGTTAGCGATGACCGTAAAGCCTCTATCGAAACTTGAATTACGAGTTGAAACGACGTCGTCTCCGTCAACGTGGCAGGATATGATAAAACGGCGTTGACGGGAACGGCGGAGGAAGAAGAGAGAGGGGAGTTGGTTGGGTTTGGGGAGATGTGGGCAGAGTGATGTGTTGGTGAGGCGTGAGAGGATGAAGGGTTGAGATGAAATTGTTGATGCCATATCGAATTTGATGATGATGATAATGGAAGAAGGAAGAAGAAGAAGAAGAAGGAAGTGACGATGGAATCTGTGTCGTTACAAAAGAAAAATGAAAACCTTCAACCTTCGCCCGAC
TARGET=1288,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0739
PRIMER_LEFT_PENALTY=0.073866
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=AGAGGATGAAGGGTTGAGATGA
PRIMER_RIGHT_SEQUENCE=TAACGACACAGATTCCATCGTC
PRIMER_LEFT=1242,22
PRIMER_RIGHT=1356,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=0.0739
PRIMER_LEFT_1_PENALTY=0.073866
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=TGAGAGGATGAAGGGTTGAGAT
PRIMER_RIGHT_1_SEQUENCE=TAACGACACAGATTCCATCGTC
PRIMER_LEFT_1=1240,22
PRIMER_RIGHT_1=1356,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.0752
PRIMER_LEFT_2_PENALTY=0.073866
PRIMER_RIGHT_2_PENALTY=0.001315
PRIMER_LEFT_2_SEQUENCE=AGAGGATGAAGGGTTGAGATGA
PRIMER_RIGHT_2_SEQUENCE=ACAGATTCCATCGTCACTTCCT
PRIMER_LEFT_2=1242,22
PRIMER_RIGHT_2=1349,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.0752
PRIMER_LEFT_3_PENALTY=0.073866
PRIMER_RIGHT_3_PENALTY=0.001315
PRIMER_LEFT_3_SEQUENCE=TGAGAGGATGAAGGGTTGAGAT
PRIMER_RIGHT_3_SEQUENCE=ACAGATTCCATCGTCACTTCCT
PRIMER_LEFT_3=1240,22
PRIMER_RIGHT_3=1349,22
PRIMER_LEFT_3_TM=60.074
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=110
PRIMER_PAIR_PENALTY_4=0.1158
PRIMER_LEFT_4_PENALTY=0.115769
PRIMER_RIGHT_4_PENALTY=0.000037
PRIMER_LEFT_4_SEQUENCE=CGATGACCGTAAAGCCTCTATC
PRIMER_RIGHT_4_SEQUENCE=TAACGACACAGATTCCATCGTC
PRIMER_LEFT_4=1068,22
PRIMER_RIGHT_4=1356,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=60.000
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_05395 Ps_contig_mira-and-tgicl-ass_franssen_37437
SEQUENCE=ACTTTTTATTGAAACTCTAAGATGATTTAGTACTAGATTCTTAAANTAATAGCTATGATTTCATGCTTAGCATGATGAATCATGAGAAGAAGTGCTAAAAGCAAGGATTGCCAGTGATTATTCTCCTTCACATACTTCCAGTTTTACATTGAAAAGGAAAAAAGAAGAAGAAAAGAATATACAAGGCTTCCACCATTATTCTCTATAAAAGTCCAACAACACAGCTCAAATGCAATCTGTTTTCCAATCCTCTCAAGTGATGACCCAATAACATACACCAGAAAAGCAACTTCGCTAGGTAATCGCGAGAAGTGGCTACTGTATCACTAAACTCATCACTGTCGTCGGGGTGATAGTCTACGTTTCCTTCTTCTGACTGTTCCATAAAGTTAGAGCAAGCTTCGTCGACAAGGAATCTTCTCATAATGTTTTGAACAAGTTGGTGAATTATATCCTCCACTTCAACGGACGAAGGTCGGGACATTTCATTCACCCTATCAGGATCAAAAGAGCGAAGATATTCCAGTAAATTATTCCGAATTCCTCTGTCATGTTCTAATTGCATCATTTCTTGCTTCTGCGCATTCAGCTCCTCCTTTGTGTTTGACAACTCAGACTGCAACTGTTGGATGTATTTCAGAGCCTGGGGCGGCAAATCTCGAAAGACTCCCGTGCCACTACTGCTGTTGCTGCAAGTTTCCGAATCATTGAATCCTGAATCAGAAACAACCTTCTTATTTTCCCCATGTTCTTCTCCTCCACCCTTGACCTCCAAACTCTCCAAAGGCGGTTCACACTCCGACACTCCATCCGCACAAGACATTTCCAGATTCCTCGTCAAGGACATCCTGTACTCCGCATTCCACAGCGTGTACCCTGTGATGATGGAAGAAACAAGCAACCGATGAAGTGGCTGAATAGAAAGACTAACAGTAACAGAGAAATGATCAGAAGGTAACAAACCAAGCATAGTGGAAATAGTTTGTTTCATAGAATCTTTAGCAGCAGTAGAAACACCTATAGAGATAACGGAGTTATCGAGGGGTTTGATACGGCGAAGCATGTTAGCGATGACCGTAAAGCCTCTATCGAAACTTGAATTACGAGTTGAAACGACGTCGTCTCCGTCAACGTGGCAGGATATGATAAAACGGCGTTGACGGGAACGGCGGAGGAAGAAGAGAGAGGGGAGTTGGTTGGGTTTGGGGAGATGTGGGCAGAGTGATGTGTTGGTGAGGCGTGAGAGGATGAAGGGTTGAGATGAAATTGTTGATGCCATATCGAATTTGATGATGATGATAATGGAAGAAGGAAGAAGAAGAAGAAGAAGGAAGTGACGATGGAATCTGTGTCGTTACAAAAGAAAAATGAAAACCTTCAACCTTCGCCCGAC
TARGET=1312,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0739
PRIMER_LEFT_PENALTY=0.073866
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=AGAGGATGAAGGGTTGAGATGA
PRIMER_RIGHT_SEQUENCE=TAACGACACAGATTCCATCGTC
PRIMER_LEFT=1242,22
PRIMER_RIGHT=1356,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=0.0739
PRIMER_LEFT_1_PENALTY=0.073866
PRIMER_RIGHT_1_PENALTY=0.000037
PRIMER_LEFT_1_SEQUENCE=TGAGAGGATGAAGGGTTGAGAT
PRIMER_RIGHT_1_SEQUENCE=TAACGACACAGATTCCATCGTC
PRIMER_LEFT_1=1240,22
PRIMER_RIGHT_1=1356,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.000
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.1158
PRIMER_LEFT_2_PENALTY=0.115769
PRIMER_RIGHT_2_PENALTY=0.000037
PRIMER_LEFT_2_SEQUENCE=CGATGACCGTAAAGCCTCTATC
PRIMER_RIGHT_2_SEQUENCE=TAACGACACAGATTCCATCGTC
PRIMER_LEFT_2=1068,22
PRIMER_RIGHT_2=1356,22
PRIMER_LEFT_2_TM=60.116
PRIMER_RIGHT_2_TM=60.000
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.1467
PRIMER_LEFT_3_PENALTY=0.146683
PRIMER_RIGHT_3_PENALTY=0.000037
PRIMER_LEFT_3_SEQUENCE=CAACGTGGCAGGATATGATAAA
PRIMER_RIGHT_3_SEQUENCE=TAACGACACAGATTCCATCGTC
PRIMER_LEFT_3=1128,22
PRIMER_RIGHT_3=1356,22
PRIMER_LEFT_3_TM=59.853
PRIMER_RIGHT_3_TM=60.000
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=0.2443
PRIMER_LEFT_4_PENALTY=0.244283
PRIMER_RIGHT_4_PENALTY=0.000037
PRIMER_LEFT_4_SEQUENCE=AGAAGAGAGAGGGGAGTTGGTT
PRIMER_RIGHT_4_SEQUENCE=TAACGACACAGATTCCATCGTC
PRIMER_LEFT_4=1176,22
PRIMER_RIGHT_4=1356,22
PRIMER_LEFT_4_TM=59.756
PRIMER_RIGHT_4_TM=60.000
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_05396 Ps_contig_mira-and-tgicl-ass_franssen_37438
SEQUENCE=TTTTAGAGAAAGTAAGAATGAATAAAGGTTGGTTCCTATTTGAGTCGATTANGAGAAACAAAAACAGGAGATTCAGGTCCGCCATTGCTAATAAAGCCAAACTTCATCYTTTCAAAAGTCAAAACATTCATTTCGACCCTGCTTCATGGATCAATAACACAGGGGACAGAGTCGGTTATATCTAGGTTAGATCATTCCATCTATTAGACAACTCCAAGTAAAGAGTTTCTTTCCTTTCTTTCTTTCATTCTTTTTTTTGGTACTTTTCTTAATATTTTAAAAAAGAAAAAGGAAATTGCAAATTAATTACATATATAACAGACAATCTGTTCAACACTGATTAACTAAGTCATATGCCAAATCATACTCCCTTTCTTTCTCGCAAGCTTCTTCCCCTCCTATAGTCAACCTCTGCATCAGAACACGATAATCCCATCGGATATTTCCTGCACATTCTGGATGACCCCATTTTTGAAAACATATCTTCATCATCCATGCGACTATGATTATCAGGTTAC
TARGET=234,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05397 Ps_contig_mira-and-tgicl-ass_franssen_37444
SEQUENCE=ACGGGGATCACATNCGTCGGGGACCGCTCAAAATTTCATCTCATCTGAGCGCTTCCTTCCGCCGCGCCGGAAATCAACACCGGCGGCGGCGGAAACCTAAACTACCGAAATCTTGCACCAGATCCTCCGTCTCCATAACCTCGACCTGAATTCCTCACTTAATTCAACCGAATCTCACGCGATCGACCTAACCCGAGCATTCCACTCACGAACCCTAAATGCAACAACTCGAATCAAACTCTCTGAACACAGAATCTAAGCTCAACCGATTGGGGATTCGATATCCCAAGCTTCTAACTACTTCGTGGCACCAAGTTTAACGAGAAACAATATAAAGATACACCTACCTGCTGGAGAAGACGGAGTCACCAGCGGAATCTCGGTTTGGCTTGGTTTTATATGGAAGCAGAGTTTCAGATTGCTTTGATCTATCCTATTGAAATTATGTAGGTGAAACTTGCTGAATGCAAGCGGGATCTCGTTTGTGGATCAAAATCCGTCATGGTCGCAACAATTATCATCTTAATTTCTCCATCAACAACATCACTAGTTGTGGTCCTGCCAGCATCACCAACTGTGTTATCCCATCATTATTCTTCGAATGCCCTGAGAAGTATCTGGCCAGTAGCACCATTAGGGCAATAACAACAACAGTGAGTCCAACAATACCAATGAAGGTGGCTAAACCATTCAAGTGGACCTGCAGAGGTGTTTCTTCACCATTATCTTCTGAAATGCTAGCCATTAGAAGGCCCCATTCATTATTAATTCCCACCTGTTACCAGCATAGTACCACTGCCATCTGCAGCCTTGCAACCAGACGTAAAAAAAAGTGTGTTTGCTGCAGTTTGGCAGGCTCTATGTGTATGGTTGATGCAAGGCGGCTTCATGAGCAAGATGTTGTTGTTTGTGCAGCAGGTTTGATGAATGCTGCTGCAATGTGTTCGCCATCATCTTGATGTTATTGTTGGTGATTTGATGATGAGCCAAAACTGCAGCATGAGGGCTCATGGAAGAGCGTAGTTTGCAGGGTCGTGGGGTGACGCTCCAGGCAGGGAGAGACATTTGGAACCCAGGTCATGGTGACTTGATGGAGGCTGGTGCAAGGCAAGCTGTAGGGCGCATGATTCTAGCATGACCTAATGATGATATTTGGTGTTCTGATGTAAAGCATTGTTGTAGGAAATAGGAAAACTGGCCAAGTATATGTGCTTGCAAGCGATATAATGTATTAATGCATCTTGTATTTTAGGTTAAGGAATGTCCATGTAATGCATGAGATTGTATGTTTGGGCTGTATGCACCATGCTTGGTTTTGATAGCATATTTGGCCTGAGGAAATGAAAAATGACTTGAGTARAAACATGGCAATACATGGTTTTAA
TARGET=81,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05398 Ps_contig_mira-and-tgicl-ass_franssen_37446
SEQUENCE=GGGGACAACGAAATAGTACAATATTTCCTTCACCTTAAGAGCCAATAATCTTCTCTTAAGCACCATCTCGTAAGAAACATGGTGTTGACAGTAGTGTCCTACACACAAAAACCTCACGTAGTTTGTGTTCCATTTCCAGCACAAGGTCATGTAAACCCTTTCATGCAATTAGCCAAAATCCTCAGAAGCATTGGTTTCCACATAACCTTTGTCAACAACGAGTTCAACCACAGACGTTTGATTAGTTCTCTAGGTGAGGACTTTGTGAAAGGTGAACCAGATTTTCAGTTCGAAACTATCCCTGATGGTTTGCCAGAATCTGATAAAGATTCAACACAAAGCATTGCTGCATTGTGTGATGGAACTAGAAAACATTGTTATGGTCCATTGAAAGAGCTTGTTAAGAAACTTAATAGCTCTTCAAATGTTCCTCAAGTTACTTCCATTATGTATGATGGACTTATGGGCTTTGCTAGAAAAGTTGCGAAAGATTTAGGTCTTGCAGATCAACAGTTTTGGACTGCTTCTGCTTGTGGCTTGTTGGGTTATTTGCAATTTGATGAGCTTGTCAAAAGAAATATCCTTCCCTTCAAAGATGAAAGCTATATCAGTGATGGATCCTTGGACAAAAATCTAGATTGGATCGAAGGAATGAAAGACATCCGAATGAGAGATCTTCCAAGTTTCGTTAGAACAACAACCTTAGACGATATCAGTTTCACTTGCTTTGGTTTCGAATCTCAGACATGCATGAAACTATCATCCTCAGTCATAATCAACACAGTCCAAGAGCTAGAAAGTGAAGTCCTTGATCATCTCATGGCCATAAATCCCAACATATACAACATAGGTCCACTTGAGTTTCTAGGTAACCATTTTCCTGGCAGAGAAAACGGTTTCAAAATCCACGGTTCAAATCTTTGGAAAAACGATGTAACATGTCTCAAATGGTTAGACCAATGGGAAGCTAACTCAGTCATATATGTAAACTATGGAAGCATAGCCGTCATGAGTGAGGAACACTTGAAGGAGTTTGCATGGGGATTAGCAAACAGTAAGTTACCGTTTTTATGGATTAAGAGACCTGATTTGGTAAAAGGAAAAGGAAAATCAACACCATTGCCACAAGAGTTTTTGGATGAGGTTAAGGATAGAGGTTACATAACAAGTTGGTGTCCTCAAAGTGAGGTACTAGGTCATTCATCGGTCGGTGCGTTTTTGACTCATTGTGGTTGGAATTCGTCGCTCGAGAGTATATGTGAAGGTAAGCCTATGATTGGTTGGCCGTTTTTCGCGGAGCAACAGACGAATTGTAGGTTTATATGCAACACTTGGGGGATTGGGATGGATATTAAGGATGATGTGAAGAGAGAAGAGGTGACTGAGCTTGTGGTGGAAATGATAAAGGGTGAAAAGGGGAATGAAATGAGAGAGAAATGTCAAGAATGGAAGAAGAAAGTGGTTGAGGCTGCTGATCCAGGTGGCTCTTCTTATGATGATTTTTATAGGTTGCTCAAGGAGGCTTTTAATTGTGATGTTGTTTGAGTTTCTAGCAACTTCTCTCAATCATAAGGTTGTCATGTGTTTTAAAATATATGTTAAATTTGTAATAATTGGCTCTAATTTGCCACTTTATGTTATGTTTCATGTTATATATGTCTTCTACAAAGGTTTGTATTTCTATGTAAAGATTATGTTTGTATGTATGTATTTTGATGATTCGGTAAAAAAATAAATGATAAGATTTTATTGTTTAANAATTATGT
TARGET=1699,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05399 Ps_contig_mira-and-tgicl-ass_franssen_37450
SEQUENCE=ACATTTAATATAGATCCATAAATAATGAATGAATGNATGAAATGAAGGAAAGAAATGAATGAAGACGCAAACGCAAAACGCAGCATAGAAAGAGAGAGAAGAAGAAGTTTGAATTTGACGTTGACGAATTGACTTACCATGGCGCCGGCGATTAAGATCGTTTTCGGACTTCTCACTTTCGTCACTATCGGAATGATTATCGGTGCTCTCTCTCAATTGGCATTCATACGAAAATTGGAAGACTCATATGGTACAGAGTCTCAACCATTTAGAAGATTACGTGGATTAGAGAGAAAAGATTACCTTAAGTTGCCTGGAGGCATTCCAAATTGGAATAATGACAAGGAAGCAGAAATATTACGCCTTGGATATATTAAACCTGAGGTGCTTAGCTGGTCTCCACGAATTATCTTACTGCATAACTTCCTGAGCTCGGAGGAATGTGATTATCTTAGGGCAGTCGCCCTCCCTCGTCTGAAAGTTTCAACAGTGGTGGATGTAAAGACTGGAAAGGGAGTCCAGAGTGATGTCAGAACAAGCTCTGGTATGTTTTTGAATGGTGAAGAGAGAAAATATCCTATGATACAAGCAATTGAAAAAAGAATTTCTGTCTATTCTCAAGTACCAGTTGAAAATGGAGAGCTTATGCAAGTCCTAAGGTATGAGAAGAATCAGTTTTACAGGCCCCATCATGACTATTTTGCTGATACCTTCAACTTGAAGCGTGGTGGGCAGCGAATAGCTACAATGCTTATGTATTTAGGTGACAATGTTGAGGGAGGAGAAACACATTTCCCAATGGCTGGTTCAGGCGAATGTAGCTGCGGTGGAAAATTGTCGAAAGGGACATGTGTGAAACCAATTAAAGGAAACGCAGTGCTTTTCTGGAGTATGGGACTGGATGGAAAGTCAGATTCGAATAGTGTTCATGGAGGATGTCCGGTACTCTCTGGGGAGAAGTGGTCAGCTACAAAGTGGATGAGGCAATCAGGTCACCATTGAATTAACTACCTATCACCAAGGGATTTCCCCCTTCATATTTTCCATTAAAAGTAGTTATTATTGATAGTATGAATATTAAATAGTCTACAGAATAATATGATATAGTTAGATACACAATGGATATCTCATTAGGTGTCTATGTTAAAGATACTAGTATATATACAGTTTCTGATAAATCTTTACTCTGAGGTCTATGGACTATTTTACG
TARGET=24,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05400 Ps_contig_mira-and-tgicl-ass_franssen_37451
SEQUENCE=TTCAACATGATTTCAGAAGTGAAATCATTATATTTTCTGTGGATGAACAAAATACAAATTATTTACAGTTTTACAGCACTTGCATGACTGTACATGTTATTACATACATACATGTACAAACCAATGGAGAGAAATATGTTAGAATGGGAGAAAACTTTGAATTATTCTGGTAAAATAGGAAGCCTAGAGACAGTGACACCATTGCCGCCACCACCAGCAGAAGCTTCTGAGGTAGAACTTCTTTTCTTGCGAGAAACAGAACCAGTTTTCGAGGAGGTTTTGGCACTAACCCTCAAGGGAAGAATCTGCAACCTCGTGTTTTGATCCGTACCTGACTTTTCAGCAGCTAACTGATAGCTTTGCACGAGTTCAAGCTGCCTGTCGATAATCTCAGAACGTCTCGGAAGAAGTTCAACGGCCTCTCCGCCAGGAATCACTATGTACTCTATTGCTAGCCGAACCTCCTCCAAGGCATCAATCTCTTCCAGAGACGGTTTTCGTTTTGGTTCTTCATCCTCAATTTCAATATCCGGAGATTCGTTTGATGGCTTCTTTGTCATGGGGCCAAAGGATTCTAGCCCAAGGATCATTTTTACGGCCTTTAGCATCCGTGCCATGGTATTGGACTTGAGAACAAATACAGGTAACTGGTGGAATTTTGCCGCACCACGGATCCATGGGTTCTGTCGTAACTCAGAACTGGACGCGAGAATCGCGTCAGCACTTCCAATGTCATCGGTTATATCTAGTAAATCTTCGAACCCCATCACTTTTGTTACTTGTTTTAGATCAGCTTCAAGCATCTAAATTCATCATCCAACAAAGAAAAACA
TARGET=101,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1056
PRIMER_LEFT_PENALTY=0.961655
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=CAGTTTTACAGCACTTGCATGA
PRIMER_RIGHT_SEQUENCE=CCTCGAAAACTGGTTCTGTTTC
PRIMER_LEFT=65,22
PRIMER_RIGHT=273,22
PRIMER_LEFT_TM=59.038
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=1.1334
PRIMER_LEFT_1_PENALTY=0.961655
PRIMER_RIGHT_1_PENALTY=0.171757
PRIMER_LEFT_1_SEQUENCE=CAGTTTTACAGCACTTGCATGA
PRIMER_RIGHT_1_SEQUENCE=AAACACGAGGTTGCAGATTCTT
PRIMER_LEFT_1=65,22
PRIMER_RIGHT_1=320,22
PRIMER_LEFT_1_TM=59.038
PRIMER_RIGHT_1_TM=60.172
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=1.1404
PRIMER_LEFT_2_PENALTY=0.961655
PRIMER_RIGHT_2_PENALTY=0.178718
PRIMER_LEFT_2_SEQUENCE=CAGTTTTACAGCACTTGCATGA
PRIMER_RIGHT_2_SEQUENCE=GGCAATGGTGTCACTGTCTCTA
PRIMER_LEFT_2=65,22
PRIMER_RIGHT_2=205,22
PRIMER_LEFT_2_TM=59.038
PRIMER_RIGHT_2_TM=60.179
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=141
PRIMER_PAIR_PENALTY_3=1.1728
PRIMER_LEFT_3_PENALTY=0.961655
PRIMER_RIGHT_3_PENALTY=0.211105
PRIMER_LEFT_3_SEQUENCE=CAGTTTTACAGCACTTGCATGA
PRIMER_RIGHT_3_SEQUENCE=CTCCTCGAAAACTGGTTCTGTT
PRIMER_LEFT_3=65,22
PRIMER_RIGHT_3=275,22
PRIMER_LEFT_3_TM=59.038
PRIMER_RIGHT_3_TM=59.789
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=1.2440
PRIMER_LEFT_4_PENALTY=0.961655
PRIMER_RIGHT_4_PENALTY=0.282356
PRIMER_LEFT_4_SEQUENCE=CAGTTTTACAGCACTTGCATGA
PRIMER_RIGHT_4_SEQUENCE=GAAAACTGGTTCTGTTTCTCGC
PRIMER_LEFT_4=65,22
PRIMER_RIGHT_4=269,22
PRIMER_LEFT_4_TM=59.038
PRIMER_RIGHT_4_TM=60.282
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_05401 Ps_contig_mira-and-tgicl-ass_franssen_37469
SEQUENCE=CCAATATTTTATCATCTAGTGTATGCACCCAGTTCACAGAAACAAAATTTAAAAAGGAGGAGACAAAATGCGGTGCAAATGACAATGGTAATGAGTGGAAAGGGTAGTAGCAGGATCATTGATGTGATATCCTAATTTCTCCACACCCTAGCCAAACTGTGCCATGAAAACAAAGCAAGCATCATAATAAGATGTCATGTCAAATAAACAACACATGAACCTAAAGGTGGAAGCAGATGCCAATGCCTGCTTGGATAAGCATTCCTAATACCAATGTATACAGAAAGTGTAATGACCATAAGGCACACAAATAAAACAAAATTAAGACCCATGCACAGCTTCTACAAATGATAACATCGTCTAACACCTATGGAAGTAATTTTGGGAACCAACACATGCTGCCAAGAAATGCATGTCTGATTAGTATAACATAGGCAAATGAATGTATATGATGCCACTGCATTCATTAACATGAGCAGGGATGATAATGGCGATGACTTGATAATCAATGGATGCATGTAACAGCTGCAAGATGCATCCTATTATGTAGTAATATTGGTTGGAGCATAAAGAACAGAGTACATCCCCTTCAAGCTACTGATAAGCCATTGCTACTGGGGCTCCGTTGGCACCAGGAGGAACAGCAATAGGGACACTCCCATCTTTTCTTTTCCCAAATGGATTTTTGGAATATTGTATCCGCATGCCAATTGAACCAGAACTTGGGATAACAGCACCCTGTAGATTGTGGTGCACATTTGTAGCACTATTCACATCTTCAAACTCAATGAAACAGACTGTATGCCTCTCCTGCCTTAAAATCTTCATTTGCTTAAAACCAGGTTGTACACTGAAAAGGCCCCTCACTTCTTCCTCATTAATGTTCTCTCCCAAGTTCCCAATAAACAGGGTGTTGCAAGGAGGATTATCTTTAGTGTTCTGAACTGCAACATAACTGCTAGGCGCTGCTATAGAAACTGAAGCAGGCATTGGTACTGGTGCAACAGGATAGCCTGCATATGGATCATAAGGAGGAGGAGGTGGTGGTGCCATATATCCATGTGGTCCCCAAACAGGTGGTGGTGGAGGATGAAAAGGAGATGGGGTTACATAAGCGGTATGTGTATAATCCCCTGCTGTTCGTAACCGCTTACTTTGATCAAAAGCACTTGCAYCNRTTCCTCTTTTAACAAACAGATTTTTCTTGGCCATCTCAGTGTGAAGAACGGACTTGGATTCATGATCGAACAGCATCTCCTGAAGAATGTCTTTGGCAGCAATCGCTTGATGAGGAGAAGTGAAAAGAGCAAAACCCATAGGCTTTTCAGCTTTGAAATTCAACTGAGAAGCTTCAAAACCCGGTAACCATCGAAGTAGGTTCTGAATTTCTCTCTCTTTTACATCTTCTGGAAGTCCCGTTATGAATATAGTTCGAACCTCTTCGGGGGAAGGAGAAGGAACGGCGGTAGGTGGAGGAGGAGCGGCGGCCGGAGGCCATTGTTGGTGGTAATGGTGGATGCCGGTGCCAGCCATGTGAAATTCCGAAAGAAACCCTAACCCTATTGCTACACCACGCACCAGTGAATCTGGTGTTTTGTTTACTCCAAACACTCTCTTCTTCNATNTTGAAGGTGTACTTGATTATTGTTTT
TARGET=1030,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05402 Ps_contig_mira-and-tgicl-ass_franssen_37476
SEQUENCE=CCTAAAAAAACTCGATAATATACTTAAGAATGTCTCATAACTAAACAAATTTAACCGACAACTTACAAAGATGTCATTTACAATTTTACATTGAAAAGCCAGCACTAGGYATAATTTAGATTCTTCAGTTACAAATACAATGAATGAACCAAATACATAACAGAAAGCCGTGAAAGAATGGGAATTTTTTCGAAAAAGAGAATCACTGCAAATCAAATGGACAAATATTCAATAAAAGACAGCCTGCAATTTTTCAAGGAGTTTCTCACCTTAAACACATCAATCACATTCACTTTACATGGGACTCTTTAATGAAAGTGACGTCCTCGGCACCAGATGATGACAGGAGGTGTTTTTCTTGCTCATCATCTGTTTCTTGTGATCTGTAGGGTGTGGCAGTTGAGAGAGAGTCCGCAAGTTCATCTTCCGAGCATTCGTGGTCTTCAGGTATTTGCTTAACAGTTGTTACTTGAAGACATTCAGGAAGTAGACAACTGCACAAAGCACCTAGCTTAGCAAGGCGGTTCACCCAACCCGGGGTCTGTTTTCCAATCAATCTATATGAGACATCATTCGTAAAATGGTTGCAGTTCTTAGATATAAGGTGATAGGTATCGCCGTGATACTCTGAAGCCATGCTTTCAATAAATGTCCGGAACTCCGAAGGGCTAATATGTATTTGACCCAAAATGATGGAACATCTGTATATGAATCCAGGGCAAGTCTTTGGCTCCGCTTCAAATACACCGCTAGCTGAGAAGTCATGGGCCCCAAATCCATACTCCTTACCATATACTTCAATCCCAGAGTGAAAGATCCCCAATCCAAACCAATAGATATAATTATTCATAGGTGTGAGATCGTACACATTCAAAACCACAAAACTGCTGTTGTTTTTGCTGCTGTTATCGTCTTCCTCATACTGTAAGCTAGAAGAAGCGCTCTCCGCTCCCATTTTTTCTTCAAACAACGGAACAGCAATTAATGGAGATGAAGGAAGAGAAGAATCTATGAGAGATCTGTTTCCTATGAGACAGATGTAGCGTACTTTGTGGCCGCGACGCTTCCTATTCCGCCTCCTCGGCACACCCAAGATTTGACTCTCTTGCTTTACTTTCTCTCAGTCTTCCTTTCTTCTTTTTATTTTTATTTTATTTTAAGA
TARGET=1145,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05403 Ps_contig_mira-and-tgicl-ass_franssen_37480
SEQUENCE=CGTAATCAAAAGTTGTAAAAACAATANAAAACCAAATACTCATTTTCTATCTGTAATTGAAAGTAAAAACAACCCAGCCTTTTTCCTANCTTTTACTAGCACNGTTCTAGTAGGTCAAATGGACACTNATCCTCAGCAATGTCTTCAGCACCATCAGTGGCAATCTGTCCTGGTGGGATAAGAGGTCTCAGTTCAGCAAACCCCCTTGCATTCTTGAACTTTCCCGTGCCCCCTATTACCGATAATCGTGACATGGTGCTTCCTATTTTATACAAACCATAAAAGTTCAAGCTATCTCCATATTCCCCTCCTTCAAACAAAGCTGTGAATACCATCATCTGTCTACTCCCATCTGCTGAACTTGCCACATACACACCTTGTGCTTTTCCAACTATCTGGGAACCTAATTCAGGTTGAGATGTAAGTATATCATCAATAACAGTGATTGTACCAAATCCTAGTCCAAGTCCATCAGGTCCTAACTGCATTTGAACATTGTTTTGGTTGTTGTTGTTTGGTGCAAAGGAAGTACCAGCCAAACCAGTTCCTAACGGGATACCGGTAACGCCATTGACGGTGGGAATAGCACCATTGGCATTAGGGATGAGAGTCCCACCTGCCGGAGTGTTGAATCCGATCGGAGTTGCAAAGGGCACTTGACCACTGTATATATTACCTAGCAAACCGGTTACGGGTCGGGCCGTTGGATTACTTCCACCAAGAATGTCATGCATGAATAGCTCAATAATGGGTTCTTTCCCTGTGGCTGCAATAGGGTCAACTGCTGTTATAGAAATTGTCAAATGAGAAAAGGTTACCAACAATGCCAATGGAGCTATAGCTAACAATCCCATAGCCATCTTGCTTCCAAGTTTTTTAAATGTAGATGATTAATCTCTGTTTGGATCAGTGCAATGGAATCAGTTTTGGGGTTATATAAGAGGGAATGAAGAGTCTGTTGGAGTAGTTGAAGCATGGATCAGTTTTATTATCTTTATTTCTTTTTTTCTAAATGCTTTAGTTATGACAAGTCAAAACATGAGTGGTGGTGTAAGTACAACTAAACCAAAATCTCAAGTCAATTGGGTGATATTGTTATTCCACATTATCATTGTCCTTTCTTTGTTAAACTATTTGAGGATTTTCAATT
TARGET=504,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0819
PRIMER_LEFT_PENALTY=0.041981
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=AACTTGCCACATACACACCTTG
PRIMER_RIGHT_SEQUENCE=GAACTGGTTTGGCTGGTACTTC
PRIMER_LEFT=358,22
PRIMER_RIGHT=546,22
PRIMER_LEFT_TM=59.958
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.0819
PRIMER_LEFT_1_PENALTY=0.041981
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=CCACATACACACCTTGTGCTTT
PRIMER_RIGHT_1_SEQUENCE=GAACTGGTTTGGCTGGTACTTC
PRIMER_LEFT_1=364,22
PRIMER_RIGHT_1=546,22
PRIMER_LEFT_1_TM=59.958
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=0.2118
PRIMER_LEFT_2_PENALTY=0.005574
PRIMER_RIGHT_2_PENALTY=0.206212
PRIMER_LEFT_2_SEQUENCE=CCTAGTCCAAGTCCATCAGGTC
PRIMER_RIGHT_2_SEQUENCE=AGGTGGGACTCTCATCCCTAAT
PRIMER_LEFT_2=456,22
PRIMER_RIGHT_2=617,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.206
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=0.2118
PRIMER_LEFT_3_PENALTY=0.005574
PRIMER_RIGHT_3_PENALTY=0.206212
PRIMER_LEFT_3_SEQUENCE=CTAGTCCAAGTCCATCAGGTCC
PRIMER_RIGHT_3_SEQUENCE=AGGTGGGACTCTCATCCCTAAT
PRIMER_LEFT_3=457,22
PRIMER_RIGHT_3=617,22
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=60.206
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=0.2118
PRIMER_LEFT_4_PENALTY=0.005574
PRIMER_RIGHT_4_PENALTY=0.206212
PRIMER_LEFT_4_SEQUENCE=TCCTAGTCCAAGTCCATCAGGT
PRIMER_RIGHT_4_SEQUENCE=AGGTGGGACTCTCATCCCTAAT
PRIMER_LEFT_4=455,22
PRIMER_RIGHT_4=617,22
PRIMER_LEFT_4_TM=59.994
PRIMER_RIGHT_4_TM=60.206
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_05404 Ps_contig_mira-and-tgicl-ass_franssen_37482
SEQUENCE=GTCGGGGGAAACACAAGCATTTATATACATAATAATAATAATATTCTAAAGTTAGAAGAAGAAAAATGAAGGGAGGTTCAATTGAACTTGGTGAAGTATCTAAAAATACATCTAGAAGCAAAGGAGTGAAAAGAGGGTTATCAATAATGGATTTTATTTTGAGAATAGTTGCTGGTGTTGCAACACTAGCTAGTGCTGTTGCTATGGCAACAACTGATGAGAGGCTTCCATTTGCTACTGCTTTTATTCAATTCAGAGCTGAATATGATGATCTTCCCTCATTTGTGTTTTTTGTAGAACGGGAAAAACTTAATTGTTTGTGGATATCTTGTGCT
TARGET=29,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1436
PRIMER_LEFT_PENALTY=1.068149
PRIMER_RIGHT_PENALTY=0.075444
PRIMER_LEFT_SEQUENCE=GGGGGAAACACAAGCATTTAT
PRIMER_RIGHT_SEQUENCE=AAAAGCAGTAGCAAATGGAAGC
PRIMER_LEFT=3,21
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=60.068
PRIMER_RIGHT_TM=59.925
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=1.2004
PRIMER_LEFT_1_PENALTY=1.068149
PRIMER_RIGHT_1_PENALTY=0.132262
PRIMER_LEFT_1_SEQUENCE=GGGGGAAACACAAGCATTTAT
PRIMER_RIGHT_1_SEQUENCE=CCTCTCATCAGTTGTTGCCATA
PRIMER_LEFT_1=3,21
PRIMER_RIGHT_1=223,22
PRIMER_LEFT_1_TM=60.068
PRIMER_RIGHT_1_TM=60.132
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=1.2072
PRIMER_LEFT_2_PENALTY=1.068149
PRIMER_RIGHT_2_PENALTY=0.139083
PRIMER_LEFT_2_SEQUENCE=GGGGGAAACACAAGCATTTAT
PRIMER_RIGHT_2_SEQUENCE=ATGGAAGCCTCTCATCAGTTGT
PRIMER_LEFT_2=3,21
PRIMER_RIGHT_2=230,22
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=60.139
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=228
PRIMER_PAIR_PENALTY_3=1.2555
PRIMER_LEFT_3_PENALTY=1.068149
PRIMER_RIGHT_3_PENALTY=0.187326
PRIMER_LEFT_3_SEQUENCE=GGGGGAAACACAAGCATTTAT
PRIMER_RIGHT_3_SEQUENCE=TTGCAACACCAGCAACTATTCT
PRIMER_LEFT_3=3,21
PRIMER_RIGHT_3=182,22
PRIMER_LEFT_3_TM=60.068
PRIMER_RIGHT_3_TM=59.813
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=1.3810
PRIMER_LEFT_4_PENALTY=1.068149
PRIMER_RIGHT_4_PENALTY=0.312895
PRIMER_LEFT_4_SEQUENCE=GGGGGAAACACAAGCATTTAT
PRIMER_RIGHT_4_SEQUENCE=TGCAACACCAGCAACTATTCTC
PRIMER_LEFT_4=3,21
PRIMER_RIGHT_4=181,22
PRIMER_LEFT_4_TM=60.068
PRIMER_RIGHT_4_TM=60.313
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_05405 Ps_contig_mira-and-tgicl-ass_franssen_37483
SEQUENCE=TTGCGTGTCTCAGAAGCAGNGGTATTTCTTAACGTCAGAGTNNGCGGGGATCTCTCTCTTTCATTCATTCATCGAAATTCAACTCTTTCTTCTTTCAACCATTTTTTCTTCTTCTTTCATTTCTTCGATCTTTTTCTTCTTCAATCAAAAATGGCCGCAAAGAACGTTGGAATTCTCGCTCTCGACATCTATTTCCCTCCCAACTGCGTTCAACAGGAAGCATTGGAGGTTCATGATGGTGCGAGCAAAGGGAAATATACCATTGGACTTGGACAAGATTGTATGTCGTTTTGTTCCGATGTTGAAGATGTTATCTCAATGAGTTTGACGGTGGTTTCTACCCTTCTTGAGAAGTATGAGATCGATCCTAAACAAATTGGTCGTCTGGAAGTAGGCAGTGAGACTGTGATAGACAAAAGCAAATCCATCAAGTCATTCTTGATGCAGATCTTTGAGAAATATGGAAATACTGACATTGAAGGTGTTGATTCAACTAATGCATGCTATGGAGGAACTGCCGCTTTGTTCAATTGTGTCAATTGGGTGGAGAGCAATTCATGGGATGGACGCTATGGACTTGTTGTCTGCACAGACAGTGCCGTCTATGCTGAAGGTCCTGCTCGACCTACCGGAGGAGCTGCTGCTATTGCCATGCTAATTGGTCCTGATGCTCCTATTTCTTTTGAAAGCAAATTGAGAGGAAGTCATATGGCTCATGTTTATGATTTTTACAAACCTGACCTTGCTAGTGAATATCCGGTTGTCGACGGAAAGCTTTCACAAACATGTTACCTCATGGCCCTTGATACTTGCTATAAAAATTTCTGTCAAAAGTATGAGAAGCACGAGGGAAAGAAATTTTCTCTTTCTGACGTTGGTTACTTTGTATTCCACTCTCCTTATAACAAGCTTGTGCAAAAAAGTTTTGCTCGTTTGGTCTTCAGTGACTTCTTGAGGAATTCCAGCTCTTCTACTGATGAGGTTGCCAAAGAAAAGCTGGGACCTTTTGCAGCTTTATCTGATGAGGACAGCTACCAAAGCCGGGATCTTGAAAAGGCATCCCAACAAGTTGCCAAGCCTCTATTTGATGAGAAGGTACAACCAACTACTTTGATACCAAAGCAAGTCGGCAACATGTACACCGCGTCCCTCTATGCAGCATTTGCATCCCTTATTCACGACAAAAATAGCTCACTGGAAGGTAAGAGGGTGATATTGTTCTCATATGGAAGTGGCCTAACTTCCACAATGTTTTCCTTGCAATTACGTGAAGCTAAACATCCATTTAGCTTGTCAAACATTGCCCGAGTGATGGATGTTGCTGAAAAGTTGAAGTCAAGACAAGAGTTCCCTCCAGAGAAGTTTATTGAAACACTGAAGCTAATGGAGCATAGGTATGGTGCCAAAGATTATGTAACAAGCAAGGACAGTAGCCTTTTATATCCAGGCACATTCTATCTCACCGAAGTTGATACCAAGTATCGCAGATTCTATGCGACAAAATCTAGTGAAAGCAATTTGACAGGAAACGGAGTGAGTGCCAATGGTCACTGATGGTTAGAGCATGTAAGCAACAGACAATAAATGGAATTAAGCTTTGGATAGGATGAAGATTAGTCTTGTTTGCATTAGTTTTTGTAAGGGAAAGCAAATGTTTTCTGCAGTTGCAATCGTTGTCAGTTCAAATATTGTATTTTACTCTTTAAAATGTTACTTTGAATTCAAAATTTTATATTTTTAGGTTATTTGTTGCATTTTAATGACTCAAAATTCAAAGAGTTGTATGCTCATGATAGTTAGCCTAGGTTATTTGTTGCATTGTAATGACTACAAAATTCAGAGAATATATATG
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1314
PRIMER_LEFT_PENALTY=1.127550
PRIMER_RIGHT_PENALTY=0.003820
PRIMER_LEFT_SEQUENCE=CGTGTCTCAGAAGCAGNGGTAT
PRIMER_RIGHT_SEQUENCE=GTATATTTCCCTTTGCTCGCAC
PRIMER_LEFT=3,22
PRIMER_RIGHT=259,22
PRIMER_LEFT_TM=61.128
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.8000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.75
PRIMER_PAIR_COMPL_END=0.75
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=1.1961
PRIMER_LEFT_1_PENALTY=1.192310
PRIMER_RIGHT_1_PENALTY=0.003820
PRIMER_LEFT_1_SEQUENCE=TGTCTCAGAAGCAGNGGTATTTC
PRIMER_RIGHT_1_SEQUENCE=GTATATTTCCCTTTGCTCGCAC
PRIMER_LEFT_1=5,23
PRIMER_RIGHT_1=259,22
PRIMER_LEFT_1_TM=60.192
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.50
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.75
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=1.3807
PRIMER_LEFT_2_PENALTY=1.376892
PRIMER_RIGHT_2_PENALTY=0.003820
PRIMER_LEFT_2_SEQUENCE=GCAGNGGTATTTCTTAACGTCAG
PRIMER_RIGHT_2_SEQUENCE=GTATATTTCCCTTTGCTCGCAC
PRIMER_LEFT_2=15,23
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=59.623
PRIMER_RIGHT_2_TM=59.996
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.75
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=1.3926
PRIMER_LEFT_3_PENALTY=1.127550
PRIMER_RIGHT_3_PENALTY=0.265053
PRIMER_LEFT_3_SEQUENCE=CGTGTCTCAGAAGCAGNGGTAT
PRIMER_RIGHT_3_SEQUENCE=AATCTTGTCCAAGTCCAATGGT
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=279,22
PRIMER_LEFT_3_TM=61.128
PRIMER_RIGHT_3_TM=59.735
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.8000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=1.4574
PRIMER_LEFT_4_PENALTY=1.192310
PRIMER_RIGHT_4_PENALTY=0.265053
PRIMER_LEFT_4_SEQUENCE=TGTCTCAGAAGCAGNGGTATTTC
PRIMER_RIGHT_4_SEQUENCE=AATCTTGTCCAAGTCCAATGGT
PRIMER_LEFT_4=5,23
PRIMER_RIGHT_4=279,22
PRIMER_LEFT_4_TM=60.192
PRIMER_RIGHT_4_TM=59.735
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.50
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_05406 Ps_contig_mira-and-tgicl-ass_franssen_37485
SEQUENCE=TGAAACCCAACCCAACCCACGTCAGTGCAAAACTAACTAACTGCTTGTTTCCCCTACGCAATCTCACCAGCAGGAACAGAAGCAGAGTCTCCAAATTCAGTTCCATCAGGAACCGGATCCAAATCCACTCCCTCACCCGGTGATGGATCATCCAGTGGAAATTCAATCAAGCTGTCAATTCCTCTGTTACTTATTTTGGCAGCAGCAACATATGCTTCAGTTTTCTGAACATATTGATTCTTCAATCAGCTGGCTACCCATTTGAAATGCTTATTTTTTTTGCTTTGTTATTACCGATTGTTGTTGTGTAATCATTTATATTATCAAGGTCCCTTGTGCTGAAGGGTTCAGTCTATTCATTTTGTAACCTCTATTGGGTTGCATCATTTTGTATTATGAGAAGGCTTTATTCTCTCCTTTATCAATAAAACACGGATTGAAATTTGTTGTGTAAC
TARGET=4,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05407 Ps_contig_mira-and-tgicl-ass_franssen_37485
SEQUENCE=TGAAACCCAACCCAACCCACGTCAGTGCAAAACTAACTAACTGCTTGTTTCCCCTACGCAATCTCACCAGCAGGAACAGAAGCAGAGTCTCCAAATTCAGTTCCATCAGGAACCGGATCCAAATCCACTCCCTCACCCGGTGATGGATCATCCAGTGGAAATTCAATCAAGCTGTCAATTCCTCTGTTACTTATTTTGGCAGCAGCAACATATGCTTCAGTTTTCTGAACATATTGATTCTTCAATCAGCTGGCTACCCATTTGAAATGCTTATTTTTTTTGCTTTGTTATTACCGATTGTTGTTGTGTAATCATTTATATTATCAAGGTCCCTTGTGCTGAAGGGTTCAGTCTATTCATTTTGTAACCTCTATTGGGTTGCATCATTTTGTATTATGAGAAGGCTTTATTCTCTCCTTTATCAATAAAACACGGATTGAAATTTGTTGTGTAAC
TARGET=31,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05408 Ps_contig_mira-and-tgicl-ass_franssen_37493
SEQUENCE=AGTACNCGGGGGATCTCAACTGTCCGCCGGGAAACGCAGCCGGAACGACCGTCAGCCCTGAATCAGCCACCGGAATCAGCCACAAAGTCCGTTCAGTTTGCAGCCTAGCTGCTATCCAACGAGAGAGAGAGGAGAGACAAAGCGAGAAGAATCATGAGGCCCATTTTGATGAAAGGCCACGAGAGGCCATTAACATTCCTCAAGTACAACAGGGATGGTGATCTTCTGTTTTCTTGCGCTAAAGACCACAACCCCACCGTGTGGTATGCTGACAACGGTGAACGTCTGGGCACTTATCGAGGCCATAATGGTGCTGTCTGGTGCTGCGATGTCTCTAGAGATTCGGGACGACTCATTACAGGAAGTGCTGACCAGACTGCAAAGCTATGGGATGTGCAGAGTGGTCAGCAATTGTTTACTTTCAATTTTGACTCGCCTGCAAGGTCTGTTGACTTTTCCGTTGGCGATAAGCTTGCAGTTATCACCACCGACCCATTCATGGAATTGACTTCGGCAATCCATGTCAAACGCATTGCAAAAGATCCTGCAGACCAGTCTGGGGAATCTTTGCTTGTCATTAAGGGCCCTCAGGGAAGAATCAATAGAGCCATATGGGGCCCCCTCAACAGAACCATTATCAGTGCCGGAGAAGACTCTATTGTTCGTATTTGGGATTCTGAGACCGGAAAACTACTTAAGGAGTCGGACAAGGAATCCGGCCACAAAAAGGCAGTAACATCACTCGCCAAATCTGCTGATGGCACACATTTCCTGACGGGCTCCCTTGATAAGTCTGCCAAGATTTGGGATAGCAGAACATTGTCTCTAATCAAGACATATGTGACAGAACGCCCTGTCAATGCAGTTGCAATGTCGCCTCTCCTTGATCATGTGGTTCTTGGAGGTGGTCAGGATGCATCAGCTGTTACAACTACTGATCATCGTGCTGGCAAATTTGAAGCCAAGTTCTACGATAAGATTCTTCAAGAAGAAATTGGAGGTGTTAAAGGGCATTTTGGACCGATTAATGCACTGGCCTTTAACCCTGATGGAAAAAGTTTTTCAAGTGGAGGTGAAGATGGATACGTACGTTTACATCACTTTGACCAGGAATATTTCAATATCAAAATTTAGTGCTCGAGAGAATTTCTGCTCATATAAAGTGTCGTTCCTAGAAGTTAGATTTCAATTCCTTTCACAATATATATTAGTGACACAGTTTTGTACTAAAGTTTTTACTGTAATCCTTCTGCTATAACATTATGAATGAATTTTGGTTGGAGTTGAACTTCCCATATTGTAAGTTTTTCATTGCANATCTTTTGATTTGGGACAAGTTTTGGCATATCTGTTTGTTTATTGCAAAGGYTTCACNAACC
TARGET=121,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05409 Ps_contig_mira-and-tgicl-ass_franssen_37498
SEQUENCE=ATTCTGTTTATTCTTGCCATTGATGATGAGAAGTATTGGCCACTGATGCCTCCATTGCCTTCATACGGGTATGGAAGGGAGCGTCCCGGGCCTCGATATGGCAGTTTGATTCATGGTCAGAATCTTAAAGATGTTGTGATAACAGGGCACAATGGCACCATAAATGGACAAGGACAAGCATGGTGGAAAAAATTTACGCCAAAAGCGTCTCAACTACACAAGGGGACCACTTGTTCAGATCATGTGGTCTAGTGACATTGTAATCACTAATATTACTTTACGTGACTCTCCTTTCTGGACACTTCATCCATATGACTGCAAAAACATTACAATAAAAGGTGTTACAATTTTGGCTCCTATAATTGATGCTCCAAATACCGACGGCATAGATCCTGATTCTTGCGAGGATATGTTGATAGAGGACTGTTACATAAGTGTGGGAGATGATGCAATTGCAATAAAAAGTGGTTGGGATCAGTATGGAATTGCTTATGGAAGACCTTCCATGAATATTATGATCCGAAACCTCGTTGTTCGCTCTATGGTTAGTGCTGGTATCTCAATAGGCAGTGAGATGTCTGGTGGGGTATCCAACATCACGGTAGAGAATCTTCGCGTGTGGGATTCTAGACGTGGCGTGAGGATAAAGACTGCACCTGGGCGAGGTGGATACGTGCGGCAAATAACTTATCGGAATATAACATTTGAGAATGTCCGTGTTGGAATTGTGATGAAAACAGATTACAATGAACACCCTGATGATGGATATGACCCTACAGCACTTCCAGATATAAGAGACATTAGCTTCACAAGCATCTATGGACATGGAGTTCGTGTGCCGGTACGTATTCATGGCAGCGAAGAGATTCCCGTGAGAAATGTAACTTTTCGAGAAATGTCAGTTGGGTTAACCTACAAGAAAAAGCACATATTTCAGTGTGCATTTGTACACGGCCGTGTAATAGGAACCATTTTCCCCTCTCCATGCGAGAACCTTGATCGGTACAATGAGCAAGGACAGCTTGTTAAACATTCCATGTCCCAGAATGTAACAGATATAGATTATGTATTTTGAGGTGTCATGTTCACTAATTCCTGGTATTGTTACTAACGTTTACCCGGGGTTCAAGTTATGTCTTGTTATTAGTATTTCTTTATTTTTTTTTTCTTTCTTTTTAGTTAAACCTTTTCCCCTTCCTTCCTTAATTTTTATAGTTCCTTTTCTAGGTAAGGTTGGGTAAGTTTAGTTAAAATTAACGTTTAATTTTTTTCAAGTATTGTAACTTTAAA
TARGET=1193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.6269
PRIMER_LEFT_PENALTY=0.053515
PRIMER_RIGHT_PENALTY=2.573367
PRIMER_LEFT_SEQUENCE=TGAGCAAGGACAGCTTGTTAAA
PRIMER_RIGHT_SEQUENCE=CCCAACCTTACCTAGAAAAGGAAC
PRIMER_LEFT=1008,22
PRIMER_RIGHT=1237,24
PRIMER_LEFT_TM=60.054
PRIMER_RIGHT_TM=60.573
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.833
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=2.7207
PRIMER_LEFT_1_PENALTY=0.147295
PRIMER_RIGHT_1_PENALTY=2.573367
PRIMER_LEFT_1_SEQUENCE=CCATGTCCCAGAATGTAACAGA
PRIMER_RIGHT_1_SEQUENCE=CCCAACCTTACCTAGAAAAGGAAC
PRIMER_LEFT_1=1034,22
PRIMER_RIGHT_1=1237,24
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=60.573
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.833
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=2.7207
PRIMER_LEFT_2_PENALTY=0.147295
PRIMER_RIGHT_2_PENALTY=2.573367
PRIMER_LEFT_2_SEQUENCE=TCCATGTCCCAGAATGTAACAG
PRIMER_RIGHT_2_SEQUENCE=CCCAACCTTACCTAGAAAAGGAAC
PRIMER_LEFT_2=1033,22
PRIMER_RIGHT_2=1237,24
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=60.573
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.833
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=2.8491
PRIMER_LEFT_3_PENALTY=0.275749
PRIMER_RIGHT_3_PENALTY=2.573367
PRIMER_LEFT_3_SEQUENCE=TGTTAAACATTCCATGTCCCAG
PRIMER_RIGHT_3_SEQUENCE=CCCAACCTTACCTAGAAAAGGAAC
PRIMER_LEFT_3=1023,22
PRIMER_RIGHT_3=1237,24
PRIMER_LEFT_3_TM=59.724
PRIMER_RIGHT_3_TM=60.573
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.833
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=2.8885
PRIMER_LEFT_4_PENALTY=0.315112
PRIMER_RIGHT_4_PENALTY=2.573367
PRIMER_LEFT_4_SEQUENCE=ATAGGAACCATTTTCCCCTCTC
PRIMER_RIGHT_4_SEQUENCE=CCCAACCTTACCTAGAAAAGGAAC
PRIMER_LEFT_4=961,22
PRIMER_RIGHT_4=1237,24
PRIMER_LEFT_4_TM=59.685
PRIMER_RIGHT_4_TM=60.573
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.833
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=277
=
PRIMER_SEQUENCE_ID=SSR_05410 Ps_contig_mira-and-tgicl-ass_franssen_37500
SEQUENCE=GTNACTGTAAAAAGACTATTGCATTTCACTTTCAATAAGATGAACAATACAATCAGGAATGGAAGTAAACAAAACAAACAACTGAGCACAAGCTTGATTAGTGAGCTACCCTAACAATAGTGTGAGCTGCCACATTACATTGGCTTGTCTAGAATTTCAAAATGCACAAAAATGTTACAAAGTGATACATGTACTTCCATACTATTTATTAATCCCTTGATCATTGACTTCATTCCTAGGAGAAAATAAATAATAGAACATCAAGATGAATGCTCAAAGCTAATTTCAACACCATTTCCACCAATTCCAGCAATGAAACATTGAAACCCGTTTGACTCCAGTTCAGCAATCACTTTATCAACAACGGTGGCGGATAGCAATGTTGGAAGCAATGTTAGAACACATCCTCCACCACCAGCTCCTGTCAACTTAGAAGCTAACTTGTACTTCAATGTTGTTTGAAGAACAGTTTCTATTGTGGCATGACTGACCCCCATACTCTGGAGCAGACCTTGATTCATTTCCATTAGCTCTTCTACCTTCTCTTCAATCTGGGTAACAGAGAGATGGTCTGGGGTAGGTGACTGGAGAACGGTCGTCAATTCTTTGCTGATAGAATCAACTGCACTAAACACAAAAGCCATGGCACCCGGGTGCCTCAGCATTCTCTCCGAAACACCAGCTACCAATGCTTTTGTGTTTCTACCTACTTTCGTGTTGGTAATGAGCATTTTAAGGTACACATTTGACTTCATCCGTGTCAAGTTACCAGATCTGAAGCTGATAATGTTACCATATGCACTGACTGTATTGTCAATTCCAGAGGGCTTTCCATGGATGATCTTCTCACCTTCAAAAGCCCATTTATTTACCAAATCAAGTTCTTTCTCCCCAAAAGAGTGCCACCCTTGTTGGCACAAATCCACAGAAACAGAATCAGTGACAGAAAGCAGAGCAGCCGCCAGAGCCACACAGAATGCCGCCGAGGAACCCAAGCCCGCACCCAAAGGAAGTTCAGAAGATATAACCACGGTAGCAGGCTTAAATCCTTGGATAGACAAGTATAACCAAAGAAAAGCAGAAACTCCAGAAGCAAGTCCAATTTTAGCCTCAGGTATACTAAGTTCTTCAACTAAAGCCGCAATTGATTTGGTAGAGTCACCAGAGCATGAAGCAGGTGTTGAAGATAACAAAGCAACATAATCAGGAAAAGCTTCTCTAATTCTACTTATCGGCCACGAGAATTCCAAATCCACATCCTTCAGAACAAGTTTCAACGATTCTTCATTTTCGGAAGAAGGAGTGGATAAGCGGAGAGAAACGTAGGTGTATAAAGCGATGGAAGAAGCAACGGCGGTTGATCCGTGAACTACGGCGTGTTCGCCGGCTAAGATGATTTTACCGGGAGCTCTGGATTTAACCTCCATTGATTGATTGATTGAAGTTTCTGGATCTTGTGATCTATGAATCTGTTACTCTCTTTTATATGACATTGTTTAGTCTGATAGAAAACGACGTCGGGTTATGGTGCTGGTTATTTCGTTGAAATGGCGATCGTATCTTACTACTGTGTCGTCTTGTATATGAAGCCGAAATAATGATCAGTGTACAGTCTCCATTGGAGTTCCATGTGGAATGCGTGTAGTTTTAATT
TARGET=1426,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1396
PRIMER_LEFT_PENALTY=0.029253
PRIMER_RIGHT_PENALTY=0.110393
PRIMER_LEFT_SEQUENCE=CAAATCCACATCCTTCAGAACA
PRIMER_RIGHT_SEQUENCE=CAACGAAATAACCAGCACCATA
PRIMER_LEFT=1247,22
PRIMER_RIGHT=1544,22
PRIMER_LEFT_TM=59.971
PRIMER_RIGHT_TM=59.890
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=0.1787
PRIMER_LEFT_1_PENALTY=0.068302
PRIMER_RIGHT_1_PENALTY=0.110393
PRIMER_LEFT_1_SEQUENCE=TTTCGGAAGAAGGAGTGGATAA
PRIMER_RIGHT_1_SEQUENCE=CAACGAAATAACCAGCACCATA
PRIMER_LEFT_1=1288,22
PRIMER_RIGHT_1=1544,22
PRIMER_LEFT_1_TM=60.068
PRIMER_RIGHT_1_TM=59.890
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.1787
PRIMER_LEFT_2_PENALTY=0.068302
PRIMER_RIGHT_2_PENALTY=0.110393
PRIMER_LEFT_2_SEQUENCE=TTTTCGGAAGAAGGAGTGGATA
PRIMER_RIGHT_2_SEQUENCE=CAACGAAATAACCAGCACCATA
PRIMER_LEFT_2=1287,22
PRIMER_RIGHT_2=1544,22
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=59.890
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=7.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.3473
PRIMER_LEFT_3_PENALTY=0.236882
PRIMER_RIGHT_3_PENALTY=0.110393
PRIMER_LEFT_3_SEQUENCE=ATAAAGCGATGGAAGAAGCAAC
PRIMER_RIGHT_3_SEQUENCE=CAACGAAATAACCAGCACCATA
PRIMER_LEFT_3=1330,22
PRIMER_RIGHT_3=1544,22
PRIMER_LEFT_3_TM=59.763
PRIMER_RIGHT_3_TM=59.890
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=0.3888
PRIMER_LEFT_4_PENALTY=0.236882
PRIMER_RIGHT_4_PENALTY=0.151911
PRIMER_LEFT_4_SEQUENCE=ATAAAGCGATGGAAGAAGCAAC
PRIMER_RIGHT_4_SEQUENCE=CGGCTTCATATACAAGACGACA
PRIMER_LEFT_4=1330,22
PRIMER_RIGHT_4=1591,22
PRIMER_LEFT_4_TM=59.763
PRIMER_RIGHT_4_TM=60.152
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_05411 Ps_contig_mira-and-tgicl-ass_franssen_37503
SEQUENCE=AAAGAGAGACTTGTTAGAATGTTGCAATCTCTCTCTCTCTCTTAATTTTCTCTCTCTATATTTCTACTTATTACTTCTACTTAATCCAGACTTGAGTTAACCCAAAGAAAACACACTCTCTCCCTCCACCGACAGTATTATTTTCTCCCAAACAACCATGACAGATCGAGTATACCCCGCCGTCAAACCCACCACCACCGCCGCCAACGGTAACGGCGTATCCACCGCCAACCCATCTTTCCCAGCCACAAAGGCCCAACTCTACGGTGCCTCTCGCCCAACCTACCGTCCTCAACCCCATCACCGCCGTACCCGCCGTCGCTGCTGCTGCACCTTCTTCTTCTACCTCCTCCTCATCATCCTCATCCTCCTCCTCATCATCGGCATCGCCGGCACTGCTTTCTACCTAATCTACCGTCCCCACCGTCCTTCCTTCACCGTCACTTCCCTCAAACTCTCCTACCTCAACCTCACATCTTCCTCCACTCTCAACTCAAAATTCAACGTCAACATCACCGCCAAAAACCCCAACAAAGACATCACCTTCGTTTACCAACCCACCTCTATTCAAATTCTCTCCAACGAAATCGATGTCGGCGACGGAACAATCCCCTCTTTCAAACACGGCAAGAAGAACACCACTCTGCTAAAAGCTTCCATTCTGAGCAAAGGAGCGCCGCTAGAGAGCGACGCCGCCACAGAGTTGAAGAAGAACATGAAGAGCAAGAACGGGTTACCGTTGAAAGTGAAATTAGATACCAAAGTGAAGGCTAAATTGGGAAAATTGAAGACACCTAACGTCAGAATAAGAGTCTCTTGTGACGGAATCAGAGTCCATGTTCCCGCCGGTAAGAAACCGGTGGCGGTAACGGCGTCAACGTCGAAGGTTAAATGTGAAGTTGACGTGAGATTTAAGATCTGGAAATGGACAGTTTAATTGATGAAGTAAACAAGTCCATGTGGAAGAAGAACACATGACATTATCATAGTCAATAGAGGTGTGTTGTGTTTTTTGTTTTTTTGTTTTTGGCTTTTTTGTTTTTATTTATTTATTTTCTGGTTT
TARGET=28,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05412 Ps_contig_mira-and-tgicl-ass_franssen_37503
SEQUENCE=AAAGAGAGACTTGTTAGAATGTTGCAATCTCTCTCTCTCTCTTAATTTTCTCTCTCTATATTTCTACTTATTACTTCTACTTAATCCAGACTTGAGTTAACCCAAAGAAAACACACTCTCTCCCTCCACCGACAGTATTATTTTCTCCCAAACAACCATGACAGATCGAGTATACCCCGCCGTCAAACCCACCACCACCGCCGCCAACGGTAACGGCGTATCCACCGCCAACCCATCTTTCCCAGCCACAAAGGCCCAACTCTACGGTGCCTCTCGCCCAACCTACCGTCCTCAACCCCATCACCGCCGTACCCGCCGTCGCTGCTGCTGCACCTTCTTCTTCTACCTCCTCCTCATCATCCTCATCCTCCTCCTCATCATCGGCATCGCCGGCACTGCTTTCTACCTAATCTACCGTCCCCACCGTCCTTCCTTCACCGTCACTTCCCTCAAACTCTCCTACCTCAACCTCACATCTTCCTCCACTCTCAACTCAAAATTCAACGTCAACATCACCGCCAAAAACCCCAACAAAGACATCACCTTCGTTTACCAACCCACCTCTATTCAAATTCTCTCCAACGAAATCGATGTCGGCGACGGAACAATCCCCTCTTTCAAACACGGCAAGAAGAACACCACTCTGCTAAAAGCTTCCATTCTGAGCAAAGGAGCGCCGCTAGAGAGCGACGCCGCCACAGAGTTGAAGAAGAACATGAAGAGCAAGAACGGGTTACCGTTGAAAGTGAAATTAGATACCAAAGTGAAGGCTAAATTGGGAAAATTGAAGACACCTAACGTCAGAATAAGAGTCTCTTGTGACGGAATCAGAGTCCATGTTCCCGCCGGTAAGAAACCGGTGGCGGTAACGGCGTCAACGTCGAAGGTTAAATGTGAAGTTGACGTGAGATTTAAGATCTGGAAATGGACAGTTTAATTGATGAAGTAAACAAGTCCATGTGGAAGAAGAACACATGACATTATCATAGTCAATAGAGGTGTGTTGTGTTTTTTGTTTTTTTGTTTTTGGCTTTTTTGTTTTTATTTATTTATTTTCTGGTTT
TARGET=1041,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05413 Ps_contig_mira-and-tgicl-ass_franssen_37512
SEQUENCE=GGGGGGGATTGATTGAGCATTTTAATTTTTTAGAGCTGAGCGAGCGAGTGTGTGAAACCGAAGATGTCTCTATCCCGCACGTGCTCTCAGTGCGGCAACAACGGCCACAACTCTCGAACATGCAACGACGGATGCAACGACGGAGGAGGAGAGAACAGCATCATGATTTTCGGCGTTCGACTCACCGGAGGAACCAACACAGCCACCAACCACACCAACCCGTTCAGAAAAAGCGCGAGCATGACCAATCTCTCTCAATACGATCAACCCACGCCTCAAGATTCCAACGCCGCGGACGCCGGATATGTCTCCGACGACATCGTTCATGCCTCCGGCAGATCCCGAGAACGCAAGCGAGGTGTTCCTTGGACGGAAGAAGAACACAAGTTGTTCTTGTTGGGATTGCAACAGGTTGGTAGAGGTGATTGGAGAGGAATTTCTAGAAGCTTTGTTAAAACTCGCACACCAACGCAGGTGGCAAGTCATGCTCAGAAATATTTCCTCCGCCGTCATAACCAGAACCGCCGCCGCCGGAGATCTAGTCTCTTTGATATCACCACCGATACAGTGGTAGAATCTTCTACTATGATGGAAGAAGATCAATTTCAGCCAGAAACGGTAGCGCCCCTGCCTCCACCTACCCCAAGCGTATATCCGTCTTCACATTACGGTGGCATCCCGGGGACACCTTTTCCGATGGGTCTTAGTCCGGTAGCATTACCGGTAATCAATGGTGAAAGAGCAGGCAAGCCAATTAGGCCAACACCAATGTTGCCTCCTTCTTCTAAGATGGCTAATTTGAATTTGAGAGACAAAGCTGCTGCATCTTCTTCTTCTTCCATTGAGCCTTTGCCATTGTCATTGAAGCTGCAACCTCCGTCCAAGGATCATTCTCCGGAAAGCAGCGGCCATTCGTCGCCGTCGGCTTTTAGAACTATGTCTGCAGGGAAGTATAGTGGTGGGGGTGGGGATAGCATTATCAGTGTTGCCTGAAAATATAGTTTGGTTATGGTTTTTTTTGGTTGACTTGGGTTTGTAAATTGAGGGCAAAGTTGGAAATATACCCAAGAGGAAAAAAAATAGAAAGACATTAGTTATTTAGTGGAGAGAATAATATAAAATAATATAAGTAGGATGGGGAAAGAGAAGATAGGGTTAGGTTATGTAGGTGATTAATTGGATGGGGAAAGATAAAATCTAAAATATTGAATGGGTGTGTTGGGGGATTCTTGTTTCTATTTGGTCTGGGGGAGTGTGAGGTTGAAGTGATAGGGCAGTAGTGGGTACTTCTGGTCTCCTATTGGTTTGTTTCTTTGCAACTTGGTGTATTGGTTTTTAAATATTGACAGAATAATATTTATGCTTTTTANNNNNYTGTTTATGCTCTCACTAGTTGGGTAGTTTTTTTATTNCAATTAA
TARGET=522,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05414 Ps_contig_mira-and-tgicl-ass_franssen_37512
SEQUENCE=GGGGGGGATTGATTGAGCATTTTAATTTTTTAGAGCTGAGCGAGCGAGTGTGTGAAACCGAAGATGTCTCTATCCCGCACGTGCTCTCAGTGCGGCAACAACGGCCACAACTCTCGAACATGCAACGACGGATGCAACGACGGAGGAGGAGAGAACAGCATCATGATTTTCGGCGTTCGACTCACCGGAGGAACCAACACAGCCACCAACCACACCAACCCGTTCAGAAAAAGCGCGAGCATGACCAATCTCTCTCAATACGATCAACCCACGCCTCAAGATTCCAACGCCGCGGACGCCGGATATGTCTCCGACGACATCGTTCATGCCTCCGGCAGATCCCGAGAACGCAAGCGAGGTGTTCCTTGGACGGAAGAAGAACACAAGTTGTTCTTGTTGGGATTGCAACAGGTTGGTAGAGGTGATTGGAGAGGAATTTCTAGAAGCTTTGTTAAAACTCGCACACCAACGCAGGTGGCAAGTCATGCTCAGAAATATTTCCTCCGCCGTCATAACCAGAACCGCCGCCGCCGGAGATCTAGTCTCTTTGATATCACCACCGATACAGTGGTAGAATCTTCTACTATGATGGAAGAAGATCAATTTCAGCCAGAAACGGTAGCGCCCCTGCCTCCACCTACCCCAAGCGTATATCCGTCTTCACATTACGGTGGCATCCCGGGGACACCTTTTCCGATGGGTCTTAGTCCGGTAGCATTACCGGTAATCAATGGTGAAAGAGCAGGCAAGCCAATTAGGCCAACACCAATGTTGCCTCCTTCTTCTAAGATGGCTAATTTGAATTTGAGAGACAAAGCTGCTGCATCTTCTTCTTCTTCCATTGAGCCTTTGCCATTGTCATTGAAGCTGCAACCTCCGTCCAAGGATCATTCTCCGGAAAGCAGCGGCCATTCGTCGCCGTCGGCTTTTAGAACTATGTCTGCAGGGAAGTATAGTGGTGGGGGTGGGGATAGCATTATCAGTGTTGCCTGAAAATATAGTTTGGTTATGGTTTTTTTTGGTTGACTTGGGTTTGTAAATTGAGGGCAAAGTTGGAAATATACCCAAGAGGAAAAAAAATAGAAAGACATTAGTTATTTAGTGGAGAGAATAATATAAAATAATATAAGTAGGATGGGGAAAGAGAAGATAGGGTTAGGTTATGTAGGTGATTAATTGGATGGGGAAAGATAAAATCTAAAATATTGAATGGGTGTGTTGGGGGATTCTTGTTTCTATTTGGTCTGGGGGAGTGTGAGGTTGAAGTGATAGGGCAGTAGTGGGTACTTCTGGTCTCCTATTGGTTTGTTTCTTTGCAACTTGGTGTATTGGTTTTTAAATATTGACAGAATAATATTTATGCTTTTTANNNNNYTGTTTATGCTCTCACTAGTTGGGTAGTTTTTTTATTNCAATTAA
TARGET=826,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05415 Ps_contig_mira-and-tgicl-ass_franssen_37521
SEQUENCE=TGCACTCTATAACCTTGCTGAAGTTAATATGGATTGCAGGTTGAAGATTGCTAGTGAGCGATGGGCAATAGATCGATTGCTCAAAGTAATTAAGACACCACATCCTGTTCCAGAAGTTTGCCGGAAAGCTGCCATGATATTAGAAAGTCTTGTTTCTGAGCCACAAAACAGAATTTTGTTGCTAGCGTATGAGAATGCTTTTTCTGAGATACTTTTCACAGATAGCAAATATTCTGACACGTTTGCGAGGATATTGTATGAACTAACATCTCGACCAAGTAACAAAGTGGCAACAGCTCGTGGCATCTGGGGCATGTAACTTCTATCCATGTAAGTGTAGATCTTTGATTTGATGCATCTGCAATTAGTGATCAGTGATATATATATAGCCCTAGTTTCACAACTTGAATAGCTTCTTTAATATCTGATTTCATTAGAAGTTTCAAAATGTATATTATGTTATGCTGTATGATTGTACCATAATGAATGTAATGAAGTTGTATGATGACAAGCTGATGCAAAATGACTTAATTTTGGAAGCTATGAACTATTGCTTTGCTATTTGCTG
TARGET=378,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6277
PRIMER_LEFT_PENALTY=0.014792
PRIMER_RIGHT_PENALTY=0.612866
PRIMER_LEFT_SEQUENCE=CGTTTGCGAGGATATTGTATGA
PRIMER_RIGHT_SEQUENCE=AGTCATTTTGCATCAGCTTGTC
PRIMER_LEFT=239,22
PRIMER_RIGHT=527,22
PRIMER_LEFT_TM=59.985
PRIMER_RIGHT_TM=59.387
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.6616
PRIMER_LEFT_1_PENALTY=0.014792
PRIMER_RIGHT_1_PENALTY=0.646803
PRIMER_LEFT_1_SEQUENCE=CGTTTGCGAGGATATTGTATGA
PRIMER_RIGHT_1_SEQUENCE=CATTTTGCATCAGCTTGTCATC
PRIMER_LEFT_1=239,22
PRIMER_RIGHT_1=524,22
PRIMER_LEFT_1_TM=59.985
PRIMER_RIGHT_1_TM=60.647
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.7564
PRIMER_LEFT_2_PENALTY=0.143555
PRIMER_RIGHT_2_PENALTY=0.612866
PRIMER_LEFT_2_SEQUENCE=CTGGGGCATGTAACTTCTATCC
PRIMER_RIGHT_2_SEQUENCE=AGTCATTTTGCATCAGCTTGTC
PRIMER_LEFT_2=306,22
PRIMER_RIGHT_2=527,22
PRIMER_LEFT_2_TM=59.856
PRIMER_RIGHT_2_TM=59.387
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.7628
PRIMER_LEFT_3_PENALTY=0.149895
PRIMER_RIGHT_3_PENALTY=0.612866
PRIMER_LEFT_3_SEQUENCE=CAAGTAACAAAGTGGCAACAGC
PRIMER_RIGHT_3_SEQUENCE=AGTCATTTTGCATCAGCTTGTC
PRIMER_LEFT_3=275,22
PRIMER_RIGHT_3=527,22
PRIMER_LEFT_3_TM=59.850
PRIMER_RIGHT_3_TM=59.387
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=253
PRIMER_PAIR_PENALTY_4=0.7904
PRIMER_LEFT_4_PENALTY=0.143555
PRIMER_RIGHT_4_PENALTY=0.646803
PRIMER_LEFT_4_SEQUENCE=CTGGGGCATGTAACTTCTATCC
PRIMER_RIGHT_4_SEQUENCE=CATTTTGCATCAGCTTGTCATC
PRIMER_LEFT_4=306,22
PRIMER_RIGHT_4=524,22
PRIMER_LEFT_4_TM=59.856
PRIMER_RIGHT_4_TM=60.647
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_05416 Ps_contig_mira-and-tgicl-ass_franssen_37522
SEQUENCE=TCGGGGATCTCCGATTGGCCCGGTCGGCGGTTCGGTCCCGATCCAATTGGCTCGTCTTCAGTGTAAAAAATATCCCCCTCTTCTCTCCACCTTCATCTTCATCTTCATCGTTTATCGCGCGTTTCATCCTCACAAACCTCACACGTGTCACCAAATCCGTTACCCCGAACCGAAAAAATCTTCAGGTTCGTATCCGATCCGTTATAACTAACTAACAAACAAAAAACCCGAGATCTTCGGTAACCGTTTCTGTTATGGAGGAGAAAGACACGTGTCCCGGCGTCGGAAGAATGAGCGCTAGATTAACCGACGTCGTTCTCGACTGCGTCTTACCGTACGTCCACGATTCCAAAGACCGAGACGCGATTTCTCAGGTCTGTAAGAGGTGGTACGAACTCGATTCGTCGACGAGGAAGCACATCACGATTGCTTTATGCTACACCACCACGCCGGACCGGCTCCGGCGACGGTTTCCTCATTTGGAATCACTGAAGCTCAAGGGAAAACCTAGAGCGGCGATGTTTAACTTGATACCGGAGGATTGGGGAGGATTTGTGACTCCTTGGGTTAGAGAGATTTCGAAATACTTCGATTGTTTGAAGTCGTTGCACTTTCGTCGTATGATTGTTACGGATTCTGATCTTCAGATTCTCGCTCGTTCTCGTCATCAAAGTCTTCATGCTCTTAAGCTTGAAAAGTGTTCTGGATTTTCAACGGATGGACTTTACTATATTTGTCACTCTTGCAAGAATTTAAGAGTCTTGTTTATGGAGGAAAGCTCAGTTGATGAGAAAGATGGTGAATGGCTGCGTGAGCTTGCTTTGAATAATACATTTCTCGAGACACTGAATTTTTACTTGACTGATATTAACAGTATCAGGATTCAGGACCTTGAACTTGTAGCAAAAAACTGCCCCAACTTGGTCTCTGTGAAAATTACTGATTGTGAAATCTTAAGTCTGGTAAACTTCTTTCGATATGCATCTTCTCTCGAAGAATTTTGTGGAGGTTCCTACAATGAAGATCCAGAAAAGTACGCTGCTGTATCATTGCCAGCAAAGTTAAATCGGTTGGGTTTGACATATATTGGAAAGAATGAAATGCCAATTGCGTTCCCTTATGCAGCCCAACTGAAAAAATTGGATCTTCTCTATGCAATGCTGGATACAGAGGATCATTGTACATTAATCGGGAAATGCCCCAACTTGGAAATCCTTGAGTCAAGGAATGTAATCGGAGACAGAGGATTAGAAGTACTTGCTCGCTGCTGTAAGAAGCTGAAAAGGCTAAGGATCGAAAGAGGTGACGACGATCAAGGAATGGAAGATGAAGATGGTATTGTTTCCCAGAGAGGACTTATTGCCCTATCACATGGCTGTCCAGAGCTCGAGTACATGGCTGTTTATGTATCCGACATTACAAATGCCTCTCTAGAACACATTGGCACTCACTTGAAAAACCTTTGTGATTTTCGCCTCGTCTTGCTAGACCGCGAGGAGAAGATAACTGATTTGCCACTTGACAATGGAGTGAGGGCTTTACTTAGAGGTTGCGAAAAGCTGAAAAGATTTGCTCTATATCTCAGACCCGGTGGCCTAACTGATGTAGGTCTAGGTTACATAGGACAATACAGTCCAAACGTGAGATGGATACTTCTCGGTTATGTCGGAGAAACAGATGCAGGGCTCTTGGAATTCTCAAAAGGTTGTCCAAGTCTTCAGAAACTTGAAATGAGAGGATGTTCATTTTTCACTGAGTATGCATTAGCTGTTGCTGCAACTAGACTAACATCTTTGAGGTATCTTTGGGTACAAGGTTATGGAGCATCAACATCAGGACTTGATCTTTTGGTAATGGCTAGACCCTATTGGAATATTGAGTTGATTCCTTCTAGAGTTGTAACTGATCATCATCATCCAGCTCATATTCTTGCTTATTACTCCCTCGCTGGACCAAGATCAGATTTTCCTGATACTGTTATACCTTTGGTTCCTGCAACAACAGCAGCATCCTATTTTGTTAACAGGTAAAGTTATGTATTTGTATATATACCAATCCCTGTTTTTCCTTTAAAAAAATTGTTTGGAAGTGGCTCAAGTGTGACAAGTGTTGTTTTAGCTAGAAGGTTTCAACTTTCACTGCTTTTTCTAGACATTGTTATTAGTCTTGTAATAAGCTTGTATTCTTTTTTGCAATTCAGATATGCTTCTATCAACTCGTGCAGCTTTATTTATTTTCCCCATTGATGTTCAATTTTGTGAATATAAAATAAGGATTRNTTTNAYYTGTTAGT
TARGET=205,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05417 Ps_contig_mira-and-tgicl-ass_franssen_37522
SEQUENCE=TCGGGGATCTCCGATTGGCCCGGTCGGCGGTTCGGTCCCGATCCAATTGGCTCGTCTTCAGTGTAAAAAATATCCCCCTCTTCTCTCCACCTTCATCTTCATCTTCATCGTTTATCGCGCGTTTCATCCTCACAAACCTCACACGTGTCACCAAATCCGTTACCCCGAACCGAAAAAATCTTCAGGTTCGTATCCGATCCGTTATAACTAACTAACAAACAAAAAACCCGAGATCTTCGGTAACCGTTTCTGTTATGGAGGAGAAAGACACGTGTCCCGGCGTCGGAAGAATGAGCGCTAGATTAACCGACGTCGTTCTCGACTGCGTCTTACCGTACGTCCACGATTCCAAAGACCGAGACGCGATTTCTCAGGTCTGTAAGAGGTGGTACGAACTCGATTCGTCGACGAGGAAGCACATCACGATTGCTTTATGCTACACCACCACGCCGGACCGGCTCCGGCGACGGTTTCCTCATTTGGAATCACTGAAGCTCAAGGGAAAACCTAGAGCGGCGATGTTTAACTTGATACCGGAGGATTGGGGAGGATTTGTGACTCCTTGGGTTAGAGAGATTTCGAAATACTTCGATTGTTTGAAGTCGTTGCACTTTCGTCGTATGATTGTTACGGATTCTGATCTTCAGATTCTCGCTCGTTCTCGTCATCAAAGTCTTCATGCTCTTAAGCTTGAAAAGTGTTCTGGATTTTCAACGGATGGACTTTACTATATTTGTCACTCTTGCAAGAATTTAAGAGTCTTGTTTATGGAGGAAAGCTCAGTTGATGAGAAAGATGGTGAATGGCTGCGTGAGCTTGCTTTGAATAATACATTTCTCGAGACACTGAATTTTTACTTGACTGATATTAACAGTATCAGGATTCAGGACCTTGAACTTGTAGCAAAAAACTGCCCCAACTTGGTCTCTGTGAAAATTACTGATTGTGAAATCTTAAGTCTGGTAAACTTCTTTCGATATGCATCTTCTCTCGAAGAATTTTGTGGAGGTTCCTACAATGAAGATCCAGAAAAGTACGCTGCTGTATCATTGCCAGCAAAGTTAAATCGGTTGGGTTTGACATATATTGGAAAGAATGAAATGCCAATTGCGTTCCCTTATGCAGCCCAACTGAAAAAATTGGATCTTCTCTATGCAATGCTGGATACAGAGGATCATTGTACATTAATCGGGAAATGCCCCAACTTGGAAATCCTTGAGTCAAGGAATGTAATCGGAGACAGAGGATTAGAAGTACTTGCTCGCTGCTGTAAGAAGCTGAAAAGGCTAAGGATCGAAAGAGGTGACGACGATCAAGGAATGGAAGATGAAGATGGTATTGTTTCCCAGAGAGGACTTATTGCCCTATCACATGGCTGTCCAGAGCTCGAGTACATGGCTGTTTATGTATCCGACATTACAAATGCCTCTCTAGAACACATTGGCACTCACTTGAAAAACCTTTGTGATTTTCGCCTCGTCTTGCTAGACCGCGAGGAGAAGATAACTGATTTGCCACTTGACAATGGAGTGAGGGCTTTACTTAGAGGTTGCGAAAAGCTGAAAAGATTTGCTCTATATCTCAGACCCGGTGGCCTAACTGATGTAGGTCTAGGTTACATAGGACAATACAGTCCAAACGTGAGATGGATACTTCTCGGTTATGTCGGAGAAACAGATGCAGGGCTCTTGGAATTCTCAAAAGGTTGTCCAAGTCTTCAGAAACTTGAAATGAGAGGATGTTCATTTTTCACTGAGTATGCATTAGCTGTTGCTGCAACTAGACTAACATCTTTGAGGTATCTTTGGGTACAAGGTTATGGAGCATCAACATCAGGACTTGATCTTTTGGTAATGGCTAGACCCTATTGGAATATTGAGTTGATTCCTTCTAGAGTTGTAACTGATCATCATCATCCAGCTCATATTCTTGCTTATTACTCCCTCGCTGGACCAAGATCAGATTTTCCTGATACTGTTATACCTTTGGTTCCTGCAACAACAGCAGCATCCTATTTTGTTAACAGGTAAAGTTATGTATTTGTATATATACCAATCCCTGTTTTTCCTTTAAAAAAATTGTTTGGAAGTGGCTCAAGTGTGACAAGTGTTGTTTTAGCTAGAAGGTTTCAACTTTCACTGCTTTTTCTAGACATTGTTATTAGTCTTGTAATAAGCTTGTATTCTTTTTTGCAATTCAGATATGCTTCTATCAACTCGTGCAGCTTTATTTATTTTCCCCATTGATGTTCAATTTTGTGAATATAAAATAAGGATTRNTTTNAYYTGTTAGT
TARGET=1906,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05418 Ps_contig_mira-and-tgicl-ass_franssen_37523
SEQUENCE=TTCTAGAACAAAGAAATTACTATCGTACTTTGTTTGTAATGGATTCCCTAACAATGTAAAATACAACCGTCCTGTGATATCCTCCACAGCTTAAAATAGGCATCTACTTTCATTGGCAACTCAACATCGATTCAAGAGATGTTTAGGAGGGTGAGTGAGCAATTCACGGCTATGTTCCGTAGAAAAGCTTTCTTGCATTGGTACACTGGAGAGGGTATGGATGAGATGGAGTTTACAGAAGCAGAAAGTAACATGAATGACCTTGTTTCCGAGTACCAGCAGTACCAAGATGCTACTGCGGATGAAGAGGGGTATGACTATGAAGATGAGGATGAAGTTCAGGAGGAGGAGGAGGAAGCTTAAACACTAGTATTTTGAACAGTGTGTTTTGTTTGTGTTGTGATATTAATGTGGTGTTTTTCTCCCTCAACCTCAACCTCAACCTCAATTCCTTTTTCTGGGTTTAACATTTTGGACACTTTGCTTGTGTTGCTATGGTAGTATTAGAGCGTACTTTTCTGCATGTTTTAATTGCTGTTAAAAGGTGCCTTATAAATTTCTGGGTTTAACATTTTGGACACTTTGCTTGTGTTGCTATGGTAGTATTAGATCGTGTTATGGGCTTGGTTCCTTGAGTGTTGAAAATCCTGTTAATCAAATCGAATGTTARTTAANACTTACANAANNRAATTTANAGAATGANAGAAAACATTGTTATTTTAGTTTTTTTTTAAAATTAAAAAAAAGTTTGG
TARGET=341,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05419 Ps_contig_mira-and-tgicl-ass_franssen_37525
SEQUENCE=CGTTNATYGGAACTTAGATAGACTTCAGAGCTAAAATTTGAGTGTGATTATCATGTTGATTGTATATTTTCAAGTAGCAACAACAACAAACAAGTCTTATCCCACTAAGCGGGGTCGGCTACATGGATCAACTTTCAAGCAAATAAGGAAATTGAATAATACAAGTCTGCAGTTGGCACAGAATACAGACTACAACTACTGTGACCAAAATTTCAATTGTGAAGTTATTTTCCCATCATACAGTGAGACCAAGGAAAAAATAGTTTTTCATCTTGAATGGATCCAGCAGGTCTGTCACAATGTTTTTTCAAGTCCTTGAGAAAGCCAGCTCCAGCAAGTGGAATCAGAGGCGATATTAGAAGTGAGTCTAGCATCAGCAATGGCACTATGAAGAGGCATCAATGATTCAACACTTCCAACAACCGATAAAATCCTTGAAACACGAGGAACTTGCGAGGAGAAAAGAAGCGGAACCGAAGGTTCTGAGCGAAGGTTCTAGAACGATTAGCAAAGGAGCTGGAGGCTACTCTCGCCGCTGATTTGCAGAACGCGGAAGCCATCGCCGATGACAGTTACCGATGGCACAGTCGATACTACCAGTAGTAGCTTGAACCCTAAACTGAACTATCATTGGGCCGG
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05420 Ps_contig_mira-and-tgicl-ass_franssen_37529
SEQUENCE=AAATGAAGAGCTTARTCAATATTATTAAAATTATATTATATATTATAATAACAAAGAGAAAACAACAACAATAGTGACTCAATCATAGAAAAACACAACCTTAAAGTGTGAACACAAACAAGGTTCATTTATAGAAACAGAACCTAACAAGTTTCACCTTCTTTATGAACCATTACTACTAAAACTACACATTCATCATTATTCCTTATCTCTCTCTCTCTCTGAACTCTCAAGCATCACAATTTCTTCATCAGATTCAATCAAACTTCAAACTCAATAATGCATCATGTTCATATCTGCAGAAGCCATGATTGGTTGATTCAAATAAGCCATATTCAAAGGGTTATTGAAGTAGTTGTTATTGTCATTATCAACACTAACAGGTTCAAAAACACCATTTGTAACATTCATGAAGTTGATGATCTCATTCAAAGACTCAAATCTGTTGTTGAGTTCATTCAATTGAGCTCTAAGCACTGAGTTCTCAGATTCAACAGCCAAGAAACGTTGAGTTGTGAGATTCACAGATGTTACAATCTGTTGATTCTCATTCCTTAGTTGACTTACTTGAAGAGCAAGATCATCTAGGTGTTTTTGTTTCCTCATTCGAGATCGCCTTGCCGATTCGCGATTCGATATCATTCTCTTTCTTTTTCTTTGATCCATTAGAAGCATCAAATCTTCCTCTGAACCTGAGTTTGGAAGCGTTGAATAGCCTGAAGATGTTCCACTTGAAGAAGCCATGTGAGATATTGCAGAAACAGAACCTAAAACAGAGGAAAACAGAGCACAAAAACAGAGGAAAACAGAGCACAAAACAGAGGACACAGGACCCAGAAACTAATATAAAGAAAAGAGGAAAAAATTGCTGAAAAATGAAAAATTATGTAATAAAAAGAGAAAAAGGATTAAGAAATAGAAATCATGAAACGTAGTAGAGCCAGTAAAGGAAAACAACAGAGAAAGATTGAACTAAATGGGTCCTGCGCCTAACAGTAGAATTGATCATACAACCAGAAAAGAAAATTTCACTCAGAATTTGATACATGAATCTCAAACATCAATAACAATATGTTAAGCATTTAAGATTTTTGAAGCTTTATTTTATGTGTCAAATCAAAATAAGCTTCAAAAATGAATTTGATGCTGAAAAAAACAGAGGAAACAGAGGTTTTCAGAGAGAGAAGGGGAAAGTGGAAGNGCCCCGCGTACTCTGCGTTNA
TARGET=209,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05421 Ps_contig_mira-and-tgicl-ass_franssen_37538
SEQUENCE=CCCGGGGTTAAACCCCGGAAAACTTAACGGGGAAAGGTTTACCAACTTTTAAACCTTTACCCTTTTTACCCTTTTCCCCCCCGGGGTAACCCGGGGGGGTACCCGGAAACCCCCTTCCGGGGAACCCGGGGAAACGTTACGGGAAGTTACCCCCCAAACCCCCCCCAAAAACCTTTAACCCAAACTTACCCCTAAAACTACCCAAACTCCCAACTCTCAATCCCACTTCCCAACTCCAACTCAAGACCCAAACCTTATCTTCACAACAACAACAGCAATTTCTTCTTCAACAACAACAACAGCAGCAACAATCTTTTCAACAAACACAAACACCCCAATCTCAATTCCAACTCCAACAGCAACAGCAACAACTTTATCAACAACAACGACTTCTACAGCAACAACCACAACCACAACCACAACCCAACCCAAGCAACAACAGCAGCAGCAACAACAGCAGAATCTTCACCAATCACTCGCTTCTCACTTCCATCTCTTACATTTGTTGGAGAATTTGGCTGAGGTTGTTGAACATGGAAACCCGGATCAGCAATCAGATG
TARGET=263,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1610
PRIMER_LEFT_PENALTY=0.101683
PRIMER_RIGHT_PENALTY=0.059316
PRIMER_LEFT_SEQUENCE=CCCTAAAACTACCCAAACTCCC
PRIMER_RIGHT_SEQUENCE=TGAAGATTCTGCTGTTGTTGCT
PRIMER_LEFT=189,22
PRIMER_RIGHT=467,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=60.059
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.1610
PRIMER_LEFT_1_PENALTY=0.101683
PRIMER_RIGHT_1_PENALTY=0.059316
PRIMER_LEFT_1_SEQUENCE=CCCCTAAAACTACCCAAACTCC
PRIMER_RIGHT_1_SEQUENCE=TGAAGATTCTGCTGTTGTTGCT
PRIMER_LEFT_1=188,22
PRIMER_RIGHT_1=467,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.059
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.1610
PRIMER_LEFT_2_PENALTY=0.101683
PRIMER_RIGHT_2_PENALTY=0.059316
PRIMER_LEFT_2_SEQUENCE=CCCTAAAACTACCCAAACTCCC
PRIMER_RIGHT_2_SEQUENCE=GAAGATTCTGCTGTTGTTGCTG
PRIMER_LEFT_2=189,22
PRIMER_RIGHT_2=466,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=60.059
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.1610
PRIMER_LEFT_3_PENALTY=0.101683
PRIMER_RIGHT_3_PENALTY=0.059316
PRIMER_LEFT_3_SEQUENCE=CCCCTAAAACTACCCAAACTCC
PRIMER_RIGHT_3_SEQUENCE=GAAGATTCTGCTGTTGTTGCTG
PRIMER_LEFT_3=188,22
PRIMER_RIGHT_3=466,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.059
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.1747
PRIMER_LEFT_4_PENALTY=0.101683
PRIMER_RIGHT_4_PENALTY=0.073038
PRIMER_LEFT_4_SEQUENCE=CCCTAAAACTACCCAAACTCCC
PRIMER_RIGHT_4_SEQUENCE=GTGAAGATTCTGCTGTTGTTGC
PRIMER_LEFT_4=189,22
PRIMER_RIGHT_4=468,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=59.927
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_05422 Ps_contig_mira-and-tgicl-ass_franssen_37538
SEQUENCE=CCCGGGGTTAAACCCCGGAAAACTTAACGGGGAAAGGTTTACCAACTTTTAAACCTTTACCCTTTTTACCCTTTTCCCCCCCGGGGTAACCCGGGGGGGTACCCGGAAACCCCCTTCCGGGGAACCCGGGGAAACGTTACGGGAAGTTACCCCCCAAACCCCCCCCAAAAACCTTTAACCCAAACTTACCCCTAAAACTACCCAAACTCCCAACTCTCAATCCCACTTCCCAACTCCAACTCAAGACCCAAACCTTATCTTCACAACAACAACAGCAATTTCTTCTTCAACAACAACAACAGCAGCAACAATCTTTTCAACAAACACAAACACCCCAATCTCAATTCCAACTCCAACAGCAACAGCAACAACTTTATCAACAACAACGACTTCTACAGCAACAACCACAACCACAACCACAACCCAACCCAAGCAACAACAGCAGCAGCAACAACAGCAGAATCTTCACCAATCACTCGCTTCTCACTTCCATCTCTTACATTTGTTGGAGAATTTGGCTGAGGTTGTTGAACATGGAAACCCGGATCAGCAATCAGATG
TARGET=288,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1610
PRIMER_LEFT_PENALTY=0.101683
PRIMER_RIGHT_PENALTY=0.059316
PRIMER_LEFT_SEQUENCE=CCCTAAAACTACCCAAACTCCC
PRIMER_RIGHT_SEQUENCE=TGAAGATTCTGCTGTTGTTGCT
PRIMER_LEFT=189,22
PRIMER_RIGHT=467,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=60.059
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.1610
PRIMER_LEFT_1_PENALTY=0.101683
PRIMER_RIGHT_1_PENALTY=0.059316
PRIMER_LEFT_1_SEQUENCE=CCCCTAAAACTACCCAAACTCC
PRIMER_RIGHT_1_SEQUENCE=TGAAGATTCTGCTGTTGTTGCT
PRIMER_LEFT_1=188,22
PRIMER_RIGHT_1=467,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.059
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.1610
PRIMER_LEFT_2_PENALTY=0.101683
PRIMER_RIGHT_2_PENALTY=0.059316
PRIMER_LEFT_2_SEQUENCE=CCCTAAAACTACCCAAACTCCC
PRIMER_RIGHT_2_SEQUENCE=GAAGATTCTGCTGTTGTTGCTG
PRIMER_LEFT_2=189,22
PRIMER_RIGHT_2=466,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=60.059
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.1610
PRIMER_LEFT_3_PENALTY=0.101683
PRIMER_RIGHT_3_PENALTY=0.059316
PRIMER_LEFT_3_SEQUENCE=CCCCTAAAACTACCCAAACTCC
PRIMER_RIGHT_3_SEQUENCE=GAAGATTCTGCTGTTGTTGCTG
PRIMER_LEFT_3=188,22
PRIMER_RIGHT_3=466,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.059
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.1747
PRIMER_LEFT_4_PENALTY=0.101683
PRIMER_RIGHT_4_PENALTY=0.073038
PRIMER_LEFT_4_SEQUENCE=CCCTAAAACTACCCAAACTCCC
PRIMER_RIGHT_4_SEQUENCE=GTGAAGATTCTGCTGTTGTTGC
PRIMER_LEFT_4=189,22
PRIMER_RIGHT_4=468,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=59.927
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_05423 Ps_contig_mira-and-tgicl-ass_franssen_37540
SEQUENCE=GGGGTAGAACTAAATCACTAAAAAGAATAATAAAAACAAAAATGCAATTTATGAAACCGGCGTTTCGTAACGGTAGCTATTGCTACGCCTACACCGTGTCCCGCTCCTTATTATTAACCTTAAACCCTAACCCTTTCTTCTTCTCTTCACCAATTCGCCCTCTCTCCATCTTCTCTTCCTCAATGGCTTCCACTCACTTCTCTTCAACCTCCGCTCTCTCTCTCGAGAAACAATTCGGCGAGTTCAGAACTCACCTCGAAGAATCTGGAACCTTGCGCGACCGAATTCGAACCGTCGTTTCGGAAATTGAGTCCACCACAAGACTCATGTATGCTAACATTCTTCTTGTTCATCACTCCCGTCCTACTCCAGAGCTTTTGGAGAAGGCTAAGTCTCAGATTGATGTTTTGAAGGACAAGTACAAGCAACTTGCTGATATTCTTGGAGGATGTCCTGGTCAATACTATAGGTATCATGGTGATTGGAAGAGTGAGACACAGTCGGTTGTTTCTATGCTTACTTTTATGCACTGGCTTGAAACGGGAACACTTCTCCAACACAAAGAAGCCGAGGAAAAACTCGGGTTGAATGGTCCGGAGGAGTTTAGTCTAGATGTTGAAGACTACCTAGTTGGTGTTTGTTTCATGTCTAATGAATTGCCAAGGTATGTGGTTAATCAAGTAACAGCTGGTGACTATGATTGTCCAAGGAAGGTCTTGAAGTTTTTAACAGATCTTCATGCTGCATTCCGAATGCTCAATCTACGGAATGATTTTCTGCGTAAGAAGTTTGATGGTAGGTTATTACTGAACTTTAACCTTGTCCAATGCATCATCTCCACCATTTTTTTCTGTCTGTTTGGTTGACATGTTTCTGCTGTTGTGACAGGAATGAAGTATGACCTAAGAAAAGTAGAGGAAGTTTACTA
TARGET=214,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0960
PRIMER_LEFT_PENALTY=0.083883
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=ACTCACTTCTCTTCAACCTCCG
PRIMER_RIGHT_SEQUENCE=CTCCAAAAGCTCTGGAGTAGGA
PRIMER_LEFT=191,22
PRIMER_RIGHT=382,22
PRIMER_LEFT_TM=59.916
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=0.0960
PRIMER_LEFT_1_PENALTY=0.083883
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=ACTCACTTCTCTTCAACCTCCG
PRIMER_RIGHT_1_SEQUENCE=TCTCCAAAAGCTCTGGAGTAGG
PRIMER_LEFT_1=191,22
PRIMER_RIGHT_1=383,22
PRIMER_LEFT_1_TM=59.916
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.1528
PRIMER_LEFT_2_PENALTY=0.151533
PRIMER_RIGHT_2_PENALTY=0.001315
PRIMER_LEFT_2_SEQUENCE=CATCTTCTCTTCCTCAATGGCT
PRIMER_RIGHT_2_SEQUENCE=GAGTAGGACGGGAGTGATGAAC
PRIMER_LEFT_2=166,22
PRIMER_RIGHT_2=368,22
PRIMER_LEFT_2_TM=59.848
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.1543
PRIMER_LEFT_3_PENALTY=0.142170
PRIMER_RIGHT_3_PENALTY=0.012153
PRIMER_LEFT_3_SEQUENCE=CCCTTTCTTCTTCTCTTCACCA
PRIMER_RIGHT_3_SEQUENCE=TCTCCAAAAGCTCTGGAGTAGG
PRIMER_LEFT_3=130,22
PRIMER_RIGHT_3=383,22
PRIMER_LEFT_3_TM=59.858
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.1806
PRIMER_LEFT_4_PENALTY=0.038361
PRIMER_RIGHT_4_PENALTY=0.142283
PRIMER_LEFT_4_SEQUENCE=TCTTCTCTTCCTCAATGGCTTC
PRIMER_RIGHT_4_SEQUENCE=TTAGCCTTCTCCAAAAGCTCTG
PRIMER_LEFT_4=168,22
PRIMER_RIGHT_4=390,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=60.142
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_05424 Ps_contig_mira-and-tgicl-ass_franssen_37543
SEQUENCE=CTAATAATGAATTTAAAAACCTCCCTGGTCCTGCTGCACCTCCTCCAAGACCCGGTATGCCTTCAATTCGTCCTCTTCCGCCACCACCTGGACTAGCTTTAAATCTACCCCGTGGTCCTATGAATAACATGCAGTATTCTAATCCTAACAACAGTGGCCTTCCAATGCCTCCACCAAGACCACCAGGCATGCACATGATGCAATCTCTCCGACCAGCTCCCCCTCCTCCAATGCAAATGTCCTCTGGACAGCATTCCATGATGGGTGGTCAACCACACCCAATGCATCCATCAATTCCTATGAATAACCAAGGAATTCCTATTCCTCCACCACCTGGATCTCAATTCACTCCTATTCCTGTTCCACGACCTTATGTTCCTCTCTCTCATCCTCCATCAGGGATGCCTATGATGCATCCACCACCTCCCCCTCAAGGATTACCACCACCACCGCCTCCTGAGGAAGCTCCTCCTCCGCTTCCAGAGGAACCAGAACCCAAGAGGCAGAAGCATGATGATTCTGCTCTGATCCCCGAGGATAAATTTCTGGCTCAACATCCGGGACCTGCTCGTATTAGTATATCGGTTCCTAATGTTGATGAAGGAAATCTCAAAGGACRAGTTCTGGAAATAACTGTTCAATCTCTGTCTGAAACTGCTGGCGGCCTGAAAGAAAAAAATTGCCGGG
TARGET=440,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05425 Ps_contig_mira-and-tgicl-ass_franssen_37545
SEQUENCE=GTAAGTNTGAGAAATTAACATGCTAGATATGACCAGAATAGTAGATTTTCAAGATACAAATAAAGGAGGTGTGTCAACAACCACATTGGAAATGGCAAATATATAAAGTAAAGAAAGCTAATCTGTTGATCTGAAGTCAAGGTTTTTGGGTACAAATCAACAACCTTTTTCCATGACTCACGCGCGGATATCTCCGTCCACCACCTCTTTACGTTCTCTCTCTCAGAAGTGAAGAGAGAAGCCGTATCATCATCAGCACTCACCAAGTAATGAATATTGGGAAGATGCGAAAGATCTGCGAGTGTGAATTCATCACCGGCCAAGTAACGAGTTTCCCCAAGCTTCCTATCGTACACATCAAGCACTTTCTTGAGCTTTTCTTTACTCTGTTTGATTGCTCCTTCGTCTTGCTTAACCTTCATTCGAGGAGCAAATGCAAGCTGAAAGACCAGAGTTGATGAGGGTGGGTTGAAGTTCTGTCCTTCAGCCTCCAACCATTGATCTATAGATGCCTTTGCAAGTGGGTTTGTACCGTACAGTTCCCTGTTTCCTTTCTCTATGTGTTTCTCACAAACATACCGGCATATGGCTCTAGACTCGAAGAGGGAAATGTGGTCATCCTGAAATGCAGGTACTTGGGCAAATGGATGGATTTTGAGGAAATCAGGGCTTCGGTGTTCTCCTTTGGACATGTTGACAGGAACAAGTTGGAATGGAATGTCTTTCTCGTTCAGACATGTCAACACTCTGGAAACCGCCGTCGATAAGGCTGGTCCGTACACTTGCACTGGAGTTGCCATTGTTCTTGTACACTTGTAACTTTCACTCAAATGCAATACTCTGCCCTTGTGATGTTGTACACTAACTTTCACTCAAATGCAATACCCTTCG
TARGET=215,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0715
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.059316
PRIMER_LEFT_SEQUENCE=TCTGAAGTCAAGGTTTTTGGGT
PRIMER_RIGHT_SEQUENCE=GCTCAAGAAAGTGCTTGATGTG
PRIMER_LEFT=129,22
PRIMER_RIGHT=374,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.059
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.1199
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.107763
PRIMER_LEFT_1_SEQUENCE=TCTGAAGTCAAGGTTTTTGGGT
PRIMER_RIGHT_1_SEQUENCE=GAAGGTTAAGCAAGACGAAGGA
PRIMER_LEFT_1=129,22
PRIMER_RIGHT_1=419,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=291
PRIMER_PAIR_PENALTY_2=0.2614
PRIMER_LEFT_2_PENALTY=0.012153
PRIMER_RIGHT_2_PENALTY=0.249232
PRIMER_LEFT_2_SEQUENCE=TCTGAAGTCAAGGTTTTTGGGT
PRIMER_RIGHT_2_SEQUENCE=CCTCGAATGAAGGTTAAGCAAG
PRIMER_LEFT_2=129,22
PRIMER_RIGHT_2=427,22
PRIMER_LEFT_2_TM=60.012
PRIMER_RIGHT_2_TM=60.249
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=0.3951
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=0.382951
PRIMER_LEFT_3_SEQUENCE=TCTGAAGTCAAGGTTTTTGGGT
PRIMER_RIGHT_3_SEQUENCE=TTAAGCAAGACGAAGGAGCAAT
PRIMER_LEFT_3=129,22
PRIMER_RIGHT_3=414,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=60.383
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=0.4473
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=0.435099
PRIMER_LEFT_4_SEQUENCE=TCTGAAGTCAAGGTTTTTGGGT
PRIMER_RIGHT_4_SEQUENCE=GATAGGAAGCTTGGGGAAACTC
PRIMER_LEFT_4=129,22
PRIMER_RIGHT_4=349,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=60.435
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_05426 Ps_contig_mira-and-tgicl-ass_franssen_37549
SEQUENCE=GGGGATTATTATTATTATCATCAGAAAAGAAAGAATGAAGAAAGAGAGAGACAGTACTATACTCAATCGTTACTCTCATCTTTTTCTTCTCTGTTTCTCTCTCCTAGGGTTGTACTTTTAGAGAGAGAATGGATTGAAAATTCACCATTCTCTCACTATTTTCCTTCATACTATTCAGCTTCTTTGCTATGGAGGACAAAGGTTCGGTTACTAAAATGATGGACTCTACATCTTCAAAGATACAACAGCTGCAAAAGGCTTTTGCTGAACTCGAAAGTTACCGAGCTGTAACTCTTAACTTGAAATGGAAAGAATTAGAGGAACATTTTCACGGGCTGGAGAAATCCTTGAAGAGGCGCTTTCATGAACTCGAAGACTTCTGCTATGGATGGGTCTTTCAATCTCCTAACTTGACGGTGTTGT
TARGET=5,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05427 Ps_contig_mira-and-tgicl-ass_franssen_37555
SEQUENCE=ATTAGTCNNNYATAGAAGAAACCACATATAGTAAATAATATATTTTACATTAATTTATACCTAGCATTATGACACAATGCAAAGTTCTTGTAAGTTTTATTTCAACTATTCTCTAACCTTATAGATCTAACTACTATTACATAATCCTGATAAATGTGAAGGAACCTTTTTGTACTACTACTACTACACTGGTTTGGTTCAAATGATAACATGTATAGGACTAACAACAACAAACAAAAAAACCAGATTTGCGAAGACACCGATGATATTTAACTCGTAAATTGTTAACCATCATCAATCAACAATCTCAAGATTGTTCTCAAACTTGTGTATCTCTGACTCTATAGTCTTGGCAATATCTGCTTCTTCCTCAGCAGCAGGTTCCGGAAATTCTACATGAAAAGATCCCTGCTTCATTGGTCCCCTAGCTTCGATTCTTGATTGTTCCTTGATGTAGTTGTAGAGTTCTTGATGGAAGGGATGATCCAAGGAATAACAATTTTCACTCCCACTTCCTGAATGTTTCGATGATCCCGAGCCTTCTTTTCTACAGAAATGCGGTAGAGATGCATAATCCATGATCTTCAACAGCTCATCTCGCCCACAACCTTGTAAGACTTGCACTTTTTTTCTAGTCCTCTCTTGTAAAAGCGGCTTCACAACCTTCCAACAACCTGAAAATATGTACGGTGCGTTTACAATGTAATAAGTATTTGTCTTCTCGGGGTAGTTCAGATCATCAATGGATGAAATTATGGTTAACAGCTTAATCTGATTCAGAGCTGATAGCTTCAGACCGGTCATATCCAAAACCTTTACGCATGTAGTAATAGGGCGCTCATGCTTCTTCGACGCAGAAGGCAAGACTACACGATCCCGATATTCATTAATTTGAATGTGAGACTGAACATAATAATGGACAGATGCTTTGTCGAATGTGCTAAGTCCAACACCGATTGCAAAGACAGGAAGACCCTCTCGTGAGTACCCTGACAATCCTATGAGCTGAGAATCGCGCACACCTCTATACAAATCTGGAGGGATTATTGGTTTCGATAATATTTTGTCAATCTCATTTTGCACTCTCCAGTTCAAACTCTCAATCAACATTTTATGAGCCTTTGAAGCATTCCATTCCCTTGCTTTTAGAAACCGAATCAAGGTTTCGATTACATGTCCTTGATGAACATTCTGAAACGTTTTCTGAAGTGGCTCCTCAACTTGATCTACAAGAGCCTGGAGTTGGTTAAGGGCATCCTGTGAAACAATCCCCATTTTTTGAGAAAATTAAGAAAAGAAAAC
TARGET=173,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05428 Ps_contig_mira-and-tgicl-ass_franssen_37559
SEQUENCE=AATGAAGAGGTGAGGTGCAGTGTGTAACACGTGTGCCTTGTCTTGTTTGCTGAATTTCTCAGTAAACTGTAAATTTACAGACACAAATGGAAGAAACAATGCAAGGGGTTTAGTTAAGTAATTACTAAGTGAGTTGAAAAGAAGAAGAAGAAGAAGCTCAATGGGTTTTGGTGGTTCCTCCTTTCCTTCTCATCTTCATCTTCTTCAAGCTTTTCTTTGAAACACACAGTTCTACCGTTAAAGAGAAGAAATTGCTGCTTACAAACCTGCTTTTCTGTACCTTATCACAAATTTGTTGATTTTGCTTTAGATGAAACCAATCTCCATACTCACTTGCTCCCTTCTCCTTTACAGGAAAAGTATAATTCAATGAATTCTACGGATGGTAAAGGAGCGCTTAACATGCTATCATTTGAAGCTGCCAAGATTAGGCTTCTGCGTAGTTTGATCATCGAGACAGAAAAAATGCAGGTTTTGGACTTTGCTGTCTTTCCAAAAGCTGAGTATGATGTCCCCATATTTTGTGCCAACTTTTTCACGTCCGCTAAAACAAATATCATTGTCTTGGACCTTAACCCCTTGCATGATATCATCAATCACGACGATTACAAGGAAAAGTACTTCAAAAGCTTAATTCCTCTTGGCTTGAAATATG
TARGET=139,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3199
PRIMER_LEFT_PENALTY=0.140441
PRIMER_RIGHT_PENALTY=0.179421
PRIMER_LEFT_SEQUENCE=GTGTAACACGTGTGCCTTGTCT
PRIMER_RIGHT_SEQUENCE=TGAAGATGAGAAGGAAAGGAGG
PRIMER_LEFT=21,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=60.140
PRIMER_RIGHT_TM=59.821
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.3468
PRIMER_LEFT_1_PENALTY=0.167348
PRIMER_RIGHT_1_PENALTY=0.179421
PRIMER_LEFT_1_SEQUENCE=AGAGGTGAGGTGCAGTGTGTAA
PRIMER_RIGHT_1_SEQUENCE=TGAAGATGAGAAGGAAAGGAGG
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=59.833
PRIMER_RIGHT_1_TM=59.821
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.3468
PRIMER_LEFT_2_PENALTY=0.167348
PRIMER_RIGHT_2_PENALTY=0.179421
PRIMER_LEFT_2_SEQUENCE=AAGAGGTGAGGTGCAGTGTGTA
PRIMER_RIGHT_2_SEQUENCE=TGAAGATGAGAAGGAAAGGAGG
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=59.833
PRIMER_RIGHT_2_TM=59.821
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.4558
PRIMER_LEFT_3_PENALTY=0.140441
PRIMER_RIGHT_3_PENALTY=0.315379
PRIMER_LEFT_3_SEQUENCE=GTGTAACACGTGTGCCTTGTCT
PRIMER_RIGHT_3_SEQUENCE=AAGCAGGTTTGTAAGCAGCAAT
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=271,22
PRIMER_LEFT_3_TM=60.140
PRIMER_RIGHT_3_TM=60.315
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=0.4827
PRIMER_LEFT_4_PENALTY=0.167348
PRIMER_RIGHT_4_PENALTY=0.315379
PRIMER_LEFT_4_SEQUENCE=AGAGGTGAGGTGCAGTGTGTAA
PRIMER_RIGHT_4_SEQUENCE=AAGCAGGTTTGTAAGCAGCAAT
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=271,22
PRIMER_LEFT_4_TM=59.833
PRIMER_RIGHT_4_TM=60.315
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_05429 Ps_contig_mira-and-tgicl-ass_franssen_37563
SEQUENCE=GAAATAAGGCGAATAGGCGTGGGAAGGGACGTCTCTGCAATAGCTTACTGTGGTGAGAATTATGGATCTACTGATTTTGTATGTTGCTTGAAAATAGTGAGAGATGCAATTTTAAAGAATGACATATTACTGGTACAATTTTATGCCACTATCTAGAGGAACTGCTGCAGTTGGATTTGTAGTTATGCTGGGTTTGTTACTTGCTGCTAATATGGAGTTCACAGGAAGCATCCCACAGGGTTTCCAAGCTGATTGGGAAGCCATTTTGAACTTGGATCCAAATTCTTTTGTGGATTCGGTCAAAAGTTGGCTATACCCATCTCTGAAGGTGACAACCTCATGGAAAGACTATCATGATGTCGCCTCAACTTTTGCAACAACAGGGTCGGTTGTTACTGCCCTGAGCACTTATGATGAATGAACTCTTGGAAACAATGGCTAGGTGAGTCCATGGCATACCGCATATTTAAGGTATCTGTATGGTTGGATAATAACAATATTGCTGCTCATTTTTCACAACCTTACGCGGAAAGCAATAGACCTGTTAGAAAGCATCGACTCAGTGATCTTCCAATTGAGGCTTTATATGTACACTATTTTTTTATTTATTTAAAGTATATCAAGTGTAATAATATGCTATTGTAATCTGTAAATTAATGTAATTTTAGGCATTAGGCGATGAAGTTACCTTTTATGTATGTTATTTCACAATAAAGAGAAACATAGCATATGAATGGAGTAATTAGGCTAC
TARGET=601,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1194
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.115007
PRIMER_LEFT_SEQUENCE=GAAACAATGGCTAGGTGAGTCC
PRIMER_RIGHT_SEQUENCE=ACTTCATCGCCTAATGCCTAAA
PRIMER_LEFT=428,22
PRIMER_RIGHT=684,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.115
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.1194
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.115007
PRIMER_LEFT_1_SEQUENCE=GAAACAATGGCTAGGTGAGTCC
PRIMER_RIGHT_1_SEQUENCE=AACTTCATCGCCTAATGCCTAA
PRIMER_LEFT_1=428,22
PRIMER_RIGHT_1=685,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=60.115
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.1194
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.115007
PRIMER_LEFT_2_SEQUENCE=GGAAACAATGGCTAGGTGAGTC
PRIMER_RIGHT_2_SEQUENCE=ACTTCATCGCCTAATGCCTAAA
PRIMER_LEFT_2=427,22
PRIMER_RIGHT_2=684,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=60.115
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.1194
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.115007
PRIMER_LEFT_3_SEQUENCE=GGAAACAATGGCTAGGTGAGTC
PRIMER_RIGHT_3_SEQUENCE=AACTTCATCGCCTAATGCCTAA
PRIMER_LEFT_3=427,22
PRIMER_RIGHT_3=685,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=60.115
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.1974
PRIMER_LEFT_4_PENALTY=0.082405
PRIMER_RIGHT_4_PENALTY=0.115007
PRIMER_LEFT_4_SEQUENCE=ATGGCATACCGCATATTTAAGG
PRIMER_RIGHT_4_SEQUENCE=AACTTCATCGCCTAATGCCTAA
PRIMER_LEFT_4=450,22
PRIMER_RIGHT_4=685,22
PRIMER_LEFT_4_TM=60.082
PRIMER_RIGHT_4_TM=60.115
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_05430 Ps_contig_mira-and-tgicl-ass_franssen_37565
SEQUENCE=CAATTGCGATCACCAGGTTCATTATTTCTTCTCCTTTTCATTGTTTCCAAATGATTCATGTAGAAAAAAGCAATCTTTTTCTTTTGTTGAGTTTGTTTACTTGTGTTGTTGTTGTTGTTCAGGAAAATGCTGATAACTTTTGTGTGTATAGAAATGAGATTCATCACTCTGTTGGAGAGCGCACTCAAGTGTTGCAGGATGTAGCTGCAGATCCAACTCTTCCTCGTACCAAGGCTGTTCGTTGCGTTCAGTGCAACCACGGTGAAGCTGTGTTTTTTCAGGCAACTGCCAGAGGGGAGGAAGGAATGACGCTATTCTTTGTTTGCTGCAACCCAAATTGTGGACACCGATGGAGAGACTGAGGTTCGTGTCTCTGCTTAAATCAAGGTTTAACTGTTGGTGAAGTCTAACATAGTTTGTAGTTATATGAAAACCTGGCATGTGTGCTACCTGTTTTAACAGTAGTTGTTAATCAAATGATTAATGGACAAATCCCTGTGGGTGTGTGTTGATAAATTTTATGGATGACCCTGAATTTTTTTGGCTTGTTAAAAAGGAAAAGGAGGGGATTGAGAAATCAAGAATCAAAATACCATTGTAANTTAAAAG
TARGET=104,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2135
PRIMER_LEFT_PENALTY=0.211181
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=GCGATCACCAGGTTCATTATTT
PRIMER_RIGHT_SEQUENCE=TTGGTACGAGGAAGAGTTGGAT
PRIMER_LEFT=5,22
PRIMER_RIGHT=231,22
PRIMER_LEFT_TM=60.211
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.2686
PRIMER_LEFT_1_PENALTY=0.211181
PRIMER_RIGHT_1_PENALTY=0.057411
PRIMER_LEFT_1_SEQUENCE=GCGATCACCAGGTTCATTATTT
PRIMER_RIGHT_1_SEQUENCE=AGCTACATCCTGCAACACTTGA
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=60.211
PRIMER_RIGHT_1_TM=59.943
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.2686
PRIMER_LEFT_2_PENALTY=0.211181
PRIMER_RIGHT_2_PENALTY=0.057411
PRIMER_LEFT_2_SEQUENCE=GCGATCACCAGGTTCATTATTT
PRIMER_RIGHT_2_SEQUENCE=GCTACATCCTGCAACACTTGAG
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=204,22
PRIMER_LEFT_2_TM=60.211
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=0.8627
PRIMER_LEFT_3_PENALTY=0.211181
PRIMER_RIGHT_3_PENALTY=0.651564
PRIMER_LEFT_3_SEQUENCE=GCGATCACCAGGTTCATTATTT
PRIMER_RIGHT_3_SEQUENCE=CTTGGTACGAGGAAGAGTTGGA
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=232,22
PRIMER_LEFT_3_TM=60.211
PRIMER_RIGHT_3_TM=60.652
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=0.8650
PRIMER_LEFT_4_PENALTY=0.211181
PRIMER_RIGHT_4_PENALTY=0.653834
PRIMER_LEFT_4_SEQUENCE=GCGATCACCAGGTTCATTATTT
PRIMER_RIGHT_4_SEQUENCE=AGCCTTGGTACGAGGAAGAGTT
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=60.211
PRIMER_RIGHT_4_TM=60.654
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_05431 Ps_contig_mira-and-tgicl-ass_franssen_37571
SEQUENCE=CTCTCTCTCTCTCTTCCTTCATTTATGGTCCCTTTCTATCCCTTTATATCTCTCTTATCACTTTCTCCATTNCTTCGGATTCATACCTATACCTTTTAAACTCAACTCCTTTTCTCCACTATATAACTTGTCTTCTGTTTCTTAATTTCCAACCAATTAACTCTTGTTTATACATATTCTCCTTCATTTGGAGTATCTTCTTTGTTATATTATAAATAATTACATAGTATTATGTCTAGCAGAAGGTCAAGACAACAATCTTCTTCTTCAAGAATTTCTGATGACCAAATCATCGAACTTGTTTCTAAGTTGCGTCAACTTGTTCCTGAAATTCGTCATAGGCGTTCAGACAAGGTATCAGCATCTAAGGTTCTACAAGAGACTTGTAACTACATAAGAAACTTACACAGAGAAGTTGATGATTTAAGTGAAAGATTGTCTCAGTTATTGGTCACAATTGATGCTGATAGTCCAGAGGCTTAATATTATCAGAAGCTTAATTAATCAATAATTAAGNTATAGTTATCTTAATTTTAATATAAATGGGAAGAAAATTTAAATATCTATTATATATTTANAAGNGTGTAGTTTGTTTAATTGGNGGGCTAGCTAGAGTGTAGTTTGAGG
TARGET=1,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05432 Ps_contig_mira-and-tgicl-ass_franssen_37578
SEQUENCE=ATCTTGAAGTTTAAAATATCACTAANNNNNATTTAAGGTAAACAAATATTGATCTTCAATTTGCCCTTCAAAGGTTGCGTTAAACATAACTTAGAAATAAATAAATAAATTTACAATGAAGCTACCATTTCGACGCACATATGACGTTACGGTACATGTGAGATACTTTAATCAATACAACATAAACCTGTCCCTAATACTCAGTTTTTTCGTTTACACCTAATCAAAACTCCTTCTTTTCAAAGCTCCCCATGTTAAGCCATATTCCCCACTTGGATGTTTCGTACCGTCATGACCTGTTAAAAGAAAAGGATTAATCTAAAGCATTAATTAATATAGCCTTATCACAAAGCATTTCGAATTGACGGCAACCAAACAAAGGAACAGGTTATTGGCGTCACAAACTGAAAACAATTTGAGTACTTGAACATAAACATAAAAATCATTTGAAGGATAATTTCAATCTAATTTACAATACATGAGGGGACCACAAAATATAAGCAATGTACGATTCTACATAGGATAGATTTCCATCACAAGGTGGTGATACCACTCAATACTCGGCGCTCACAAATTGGATCCCACAAAATCAGAGATGAATGAAATTCTCCAACGGATTGATCCTAACAGCAAGAAAAAAGCTCACAAAATCTAAGGGCACCTCACCCCAAGTTTAAATTTGAAGCTATCCTATAAAAGGAGACTAGTAGAAGGAATTAAGGGGGGGAGAATACTACTGTTACGCTGCTGATTTTTCTTCTTGGACAAATTCTACCAAAGAACGTAAGGACAACTCGGAGTTTCTCAGTGAAACTCCAAGTGAGCTAAACTCACCCCACAACAAGGAAAAGAAATGCGCGCTGAGAGAGAAGAAGAAAGAGGACGTCGTAAAGCAGAAGGGATTGACTTATCTGAAGGTCGTCAGAGAGAACGTCGTCGAACTAGGATCAGGTAAATTTCTTCACGTTTGAATCCAAATTTATTGCGCCTTCATGTTCACCATATTTAACTGAGTAGGAGCCCTCTATTGGCTGCTTGGATTGTTGGAGAATTTGAGTCGATTTTTTTTTGGATTTTTGTGGATTTTAGGGTTTTGATATAGATCGCGCCGTTTGGATTTTTGAGGGGTTATTAGTGAAATATATGGTTAGATTTGGACTTAGGAAGATTTGGTTGGGTAGGATAGAAGGTCCGGCTAATTTATGGGTAGCCCACGGCGGCGCCGCCGCGGTGTTCTTGAGCGCCACCATGTTCTTCATGTCCTCCGCCTTGGAGTTCTTGCGCTGACACCATGTTTAATCTTTTTTTCTTGTGTTTTAATGTAAGGTTTCTGTTTTGGATATAGAGAGTTAGAAGATGAGAGAGGAGCCGTTTCATGTGTTAACGTNAGGTT
TARGET=95,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4067
PRIMER_LEFT_PENALTY=1.351760
PRIMER_RIGHT_PENALTY=0.054900
PRIMER_LEFT_SEQUENCE=CCTTCAAAGGTTGCGTTAAACA
PRIMER_RIGHT_SEQUENCE=AAACATCCAAGTGGGGAATATG
PRIMER_LEFT=64,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=61.352
PRIMER_RIGHT_TM=59.945
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=1.4321
PRIMER_LEFT_1_PENALTY=1.351760
PRIMER_RIGHT_1_PENALTY=0.080323
PRIMER_LEFT_1_SEQUENCE=CCTTCAAAGGTTGCGTTAAACA
PRIMER_RIGHT_1_SEQUENCE=TTTAACAGGTCATGACGGTACG
PRIMER_LEFT_1=64,22
PRIMER_RIGHT_1=303,22
PRIMER_LEFT_1_TM=61.352
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=240
PRIMER_PAIR_PENALTY_2=1.5866
PRIMER_LEFT_2_PENALTY=1.351760
PRIMER_RIGHT_2_PENALTY=0.234845
PRIMER_LEFT_2_SEQUENCE=CCTTCAAAGGTTGCGTTAAACA
PRIMER_RIGHT_2_SEQUENCE=GGGAGCTTTGAAAAGAAGGAGT
PRIMER_LEFT_2=64,22
PRIMER_RIGHT_2=249,22
PRIMER_LEFT_2_TM=61.352
PRIMER_RIGHT_2_TM=60.235
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=1.6148
PRIMER_LEFT_3_PENALTY=1.351760
PRIMER_RIGHT_3_PENALTY=0.263023
PRIMER_LEFT_3_SEQUENCE=CCTTCAAAGGTTGCGTTAAACA
PRIMER_RIGHT_3_SEQUENCE=AAGTGGGGAATATGGCTTAACA
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=273,22
PRIMER_LEFT_3_TM=61.352
PRIMER_RIGHT_3_TM=59.737
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=1.6511
PRIMER_LEFT_4_PENALTY=1.351760
PRIMER_RIGHT_4_PENALTY=0.299343
PRIMER_LEFT_4_SEQUENCE=CCTTCAAAGGTTGCGTTAAACA
PRIMER_RIGHT_4_SEQUENCE=GAATATGGCTTAACATGGGGAG
PRIMER_LEFT_4=64,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=61.352
PRIMER_RIGHT_4_TM=59.701
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_05433 Ps_contig_mira-and-tgicl-ass_franssen_37581
SEQUENCE=CTTCTTCCACTTCGTTTGTTCCTCTAACGCCTCCAATCTAAACGCCACAATCTCAACCTCTTTCCCATATCTCAAATTCCATCTCTACACCTTCGACGACACCACCGTCTCTGGCCTCATCTCAACTTCAATCCGTTCAGCTCTCGATTGTCCTCTCAACTAYGCTCGTACCTACCTACCAAATCTCCTACCACTTTCCGTTACCCGCGTCGTCTATCTCGATTCCGACCTTATTCTCGTCGACGACATTGNCAAAACTAAGACACAAACAACCGTTAGGAGAAGACTCTGTTTTAGTCAGCACCGGAATACTGCAACGCGAATTTCACATCCTATTTCACTCCATCGTTCTGGTCTAACCCTTCTCTGTCTCTAACGTTTGCTAACAGAAAGCCTTGTTATTTCAACACGGGTGTGATGGTGATTGATCTAGAAAGATGGAGAGAAGGGGATTATACTTCAAAGATCGAGGACTGGATGGAGTCG
TARGET=169,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05434 Ps_contig_mira-and-tgicl-ass_franssen_37612
SEQUENCE=TTGGAACATTTCCAATTTGAAGTAACATTAAGTGGCCATAGATATAAGAGTCTCCTTGCCAGAGTATAAGAGTGAAATCAGATTATGCAAGTGTTTGCATGAACAGATATAGTCGAGAAAGAAATAAAAATAATTTACGCACTAATGATACAAAAATACAGGGAATCATTAAGTTGTTTTCCAACCCACAAAATAAAACAAAATATGAAGGTAGTTTTATTTGTTGTTGATCAGTGTCTAAGAACCGTTAGCTGGAGGTGTAGAAGACTTCCCCCCCTCAATGTCGATCTCTTTTACACTATCACTTAGTGAACATGATTCACATTTCTTCTCATCCTTGTCAGGTTCAGTATTATCATCCTCGTCACTTTCATCTTCAACATGAGAACTCTTGTTTCGATGCTCTTTCACTTTAATAAATTCATCATAATGGGCCTTTCTTAGCTTTCTAAAACTTCTGCTCCTATCATCATCATCTTGTTCCATTGCTTCTAGCTCATCCTCAGATGATGTCCAACCAAATGATTGTCCGGGGCCTCTTTTACTACTAGAGCCTGCATCAGTCAGAGCAGTCCATATTGCTTCAACTTGTGTTGTGCGATTTTCATCATCAATACGTGCATCAAAACTTCCTCGTACAGGGGATAGAGAACAATCGTCATCAACCATAGGATGATATGGAGTCTTAGGTTCAGTGATTTTCTGCCTCACAGGCTTATTTGCCTCAATATCTCCAATGTTAGCTTCATCCCACCTTACACGTCCCTTCATTTTGCTAGACAGATTAGCTCTTCAAACACCAAAAGCATGCAACAAAATCCTCAAGTGGCTTGATCAACCTTAAAATTGAAATGGAAAATCGATTGTGGTGTTAAGTTGCTGCACAAGTCAAATAGTGCGATGAAAAAAAGCGTTTTATATTAATGGAAAATCTTCTTTNCTCCATCCCCCG
TARGET=466,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2428
PRIMER_LEFT_PENALTY=0.147295
PRIMER_RIGHT_PENALTY=0.095523
PRIMER_LEFT_SEQUENCE=TCCTCGTCACTTTCATCTTCAA
PRIMER_RIGHT_SEQUENCE=GCAATATGGACTGCTCTGACTG
PRIMER_LEFT=358,22
PRIMER_RIGHT=581,22
PRIMER_LEFT_TM=59.853
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.2665
PRIMER_LEFT_1_PENALTY=0.228115
PRIMER_RIGHT_1_PENALTY=0.038361
PRIMER_LEFT_1_SEQUENCE=AATGGGCCTTTCTTAGCTTTCT
PRIMER_RIGHT_1_SEQUENCE=TGACGATTGTTCTCTATCCCCT
PRIMER_LEFT_1=428,22
PRIMER_RIGHT_1=660,22
PRIMER_LEFT_1_TM=59.772
PRIMER_RIGHT_1_TM=59.962
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.2665
PRIMER_LEFT_2_PENALTY=0.228115
PRIMER_RIGHT_2_PENALTY=0.038361
PRIMER_LEFT_2_SEQUENCE=AATGGGCCTTTCTTAGCTTTCT
PRIMER_RIGHT_2_SEQUENCE=GACGATTGTTCTCTATCCCCTG
PRIMER_LEFT_2=428,22
PRIMER_RIGHT_2=659,22
PRIMER_LEFT_2_TM=59.772
PRIMER_RIGHT_2_TM=59.962
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.3236
PRIMER_LEFT_3_PENALTY=0.228115
PRIMER_RIGHT_3_PENALTY=0.095523
PRIMER_LEFT_3_SEQUENCE=AATGGGCCTTTCTTAGCTTTCT
PRIMER_RIGHT_3_SEQUENCE=GCAATATGGACTGCTCTGACTG
PRIMER_LEFT_3=428,22
PRIMER_RIGHT_3=581,22
PRIMER_LEFT_3_TM=59.772
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.3821
PRIMER_LEFT_4_PENALTY=0.147295
PRIMER_RIGHT_4_PENALTY=0.234845
PRIMER_LEFT_4_SEQUENCE=TCCTCGTCACTTTCATCTTCAA
PRIMER_RIGHT_4_SEQUENCE=ATCCCCTGTACGAGGAAGTTTT
PRIMER_LEFT_4=358,22
PRIMER_RIGHT_4=645,22
PRIMER_LEFT_4_TM=59.853
PRIMER_RIGHT_4_TM=60.235
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_05435 Ps_contig_mira-and-tgicl-ass_franssen_37639
SEQUENCE=TTCGGGGTTTATTACTTGTAACCAGACCCATAAAAATGGGACTATCCACCTCTATATTTCCATAAACAATAAAGGAATGTGACAAAATAGGAATTTAAGACCAAAGATTAGAAGATTGAGTCTGCATTAGTAGCCAGTGCATCACGTAGAGTAAAAAAAAGTCATAAATATATAAAGCAGAAATGAATAAAATTAATTAACATAAACTCTTATAACTAATGCTATCACAGTTTGGTTCTAACGGTACAATAGAGAATGTAAAAAAAGTAATTAAATATACTAAAACACGCTTGAGAAACTACGAACAATCTAGAGCCATATAAATAAAGACGCCAATCTATAATAAGCTTTATCAATTAGAAAATGACACAATCTACAATAACAAGGACACAATCAAAATCTAGGGTTACTCTAATATGTGAAATATTAAAAACTCATTCCATTTAAGTTTAATCTCTCCCATTAAAATCACAAATTAAAAACCCAAAAATCAAAAGACAGCATTAGAATAAACTGAGAAGGAAAACTCGCTAAAGAAAGGACGAGAAAGAGAAAGAAGAACAAAATAGTGATGAAGATGAAATGTTTTGAACTGCATAGCCCGATCATTGGAGAATATAAATATTTTAAAGGCACAGGCCACAAAACTACTGTACCAACGAATAACAAGTTTATGTCTCATTGCAAGGATTTGACTTATTTGATGAAAGTTGTTTTTCATTTTCACAACTTAAAAAAAGTATTATGTATTTCCTNNTTTCCCATTTATTATAACTTATAGTNGTTTTCTTGCATTGTTCTTTTAACTTTAAAAAGTTTTAAAAAAAGTTAAAAATTAAAATTTAAGTTAAACGTAACTAACTAACTTACTTTA
TARGET=855,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05436 Ps_contig_mira-and-tgicl-ass_franssen_37643
SEQUENCE=ATGGCTTATTTAGTCTTCTTCTTCATTTTCTTCTTNGTCTTTCTCTTCCTTCTCAGAAAACTCCGTTACGGTAGACTCCGACTACCGCCGGGGAATCTCGGTTTACCGTTCATCGGAGAAACCTTACAGATGATATCAGCATACAAGACTGACAATCCAGAGCCTTTTCTGGATCAGCGAGTCAACCGATACGGTTCAGTCTTCACTAGCCATGTTTTTGGTGAACCGACAGTTTTCTCGACTGATCCAGAAACGAACCGGTTCATTCTCACGAACGATAAGCTTTTCGAATGTAGCTACCCCGGTTCAATATCGAACCTTCTCGGAAAACACTCGCTGCTTCTCATGAAAGGCTCTCTTCACAAGAAAATGCATTCTCTCACCATGAGCTTCGCTAATTCGTCCATCATTAAAGATCATCTTCTTTATGATATAGACCGACTCATCCGGCTCAACATAGATTCTTGGTCCGACCGGATTCTTCTCATGGAGGAAGCTAAGAAGATAACATTTGAGYTGACAGTGAAACAGTTGATGAGTTTTGATCCAGATGAATGGACAGAGAGTTTAAGGAAAGAGTACATGCTTGTAATTGAAGGCTTTTTCACTATTCCTTTTCCTCTTCTGTCTCCCACCTACCGCAGAGCCATCAAGGCAAGAACGAAGGTGGCAGAGGCACTGACAGTAGTTGTGAGGAATAGGAGAAAAGAGTTTGAGACAAAGGAAGAGAAAAAGAATGACATGCTTGGAGCATTGTTGGCATCAGGTGAACAATTCTCGGACGAGGAAATTGTTGATTTTATGTTGGCTTTGCTTGTAGCTGGTTATGAAACTACTTCTACTATNATGACTCTTGCCATCAAGTTCCTCAATGAGACTCCTTTGGCTTTGGCACAGCTCAAGGAAGAACATGAACAAATCAGAGCAAGGAGTGAGCCAGGGGCAGCACTGGAATGGACAGATTACAAATCAATGGCATTTTCTCAATGTGTTGTGAATGAGACCTTAAGGGTGGCAAACATAATCGGTGCGATATTTAGGAGAACAACAACAGACATCGACATAAAAGGTTACACTATTCCAAAGGGATGGAAGGTCATTGCATCGTTTCGTGCTGTGCATTTGAATCCAGAATTATTTCAAAGATCGCCCGCACCTTCAATCCATGGAGATGGCAGAGTAATAATAACTCAGAAGCTGTAGCAACCCCTGGGAATGTATTTACACCATTTGGAGGAGGACCAAGGTTGTGCCCGGGCTATGAACTTGCAAGAGTTGTTCTTTCGGTTTTTCTTCACCGCTTTGTCACGCGCTTCAGTTGGGTTCCNGCTGAAGAAGATAAGTTGGTGTTTTTCCCAACTACTAGGACACAGAAGAGGTATCCTATTATATTGAAGCNGAGAGAAGAATCGAGATCAAACATGGGATTTGGGATCACAGCAGAAACAGAAAGGCGAAAAGAGAGCTGATAGTATAGATTAGTTTAAGCAGATCATGTGCATTCCATATATAAAATAAATAAATAGTTATTATTAATAAAAAACTTACTGCTAGTTTAGAAATCTGTATAGGATTTAATNAATTTNATATAATNTTNAAGA
TARGET=1513,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05437 Ps_contig_mira-and-tgicl-ass_franssen_37645
SEQUENCE=CGAGAAAACGAATAGTTGTAAATTCAAATTTTCTTGACAACATATCAAAATATTAATCTATTTGAACTATACTTACAAAGCCAGATAAATACATTTTACCTATTACATCTGCGACTGGATTAGCTACTAACTAAGTATATAAAAATACACAATCTAAAACCGTTTATCTCTTATGTTCCATGGTTGCTTCACAGGCTTCACATATAGTTGACAGAGACTGCAACCAAGTGAGTACAAAAGAGGGACCCTTTTCATAAATAATAATAACCATACCTTGTTTTGGAGTTGGTTCTAAAATCTCTCTTCGATCATAAATGTTCACAAATCACATAATAGTTACTTAGCATCAACGTACATTGAACTGACCCTAGCATCTCATCAATTAAATTCTTCAAGACAGTAGAAACAAACCAAACCAAACCAAAACCTAGGTTAAGATAAACCAAACTACTAGAAACCAATTATTTAAAATTCAAAGATATTGTTATAAAAAATGAAATAGTGCAGAAGACAAGTTGAGTAGATTGCGACAGTACAACAGATAGGACCACCACAAACAGTTGTCTATGGAAGAAAGTAACAAATGAAACCGTTTCAAATTTTGTCTGTGCAGTACTCGTGCTAAATGCATCCCAAAAAAGTTAGATAAAGGTTGTTGAGATTACGAGACAAATAATCAGTACCACTTAAAAAGAAGAAAAGAAAATGGATTTATACTTTGTCTTGCATTAATGTCCATATATATTTCTTCTTCTGTTATATGTATATATACACACATAAATTATGTATTAATTATTAATAGTAATAAACAAATACGTGCATGTGATTGTGAATGTGTGTAGCTGGTATAAAGTAATTTATAATTAATGACAAGAGTTAAAGCACATGTATTTGCACATGTAAATTTCAAAATATTTCATAAATAAAACTTGTATAAATCTATAGGATAAGGATTAGAGATTTATTTACCTGAAATTCAACAGATCGAAATGGAGGAAGTTAGTTATCTGTTGATAATTGAGTGATTGAAGCTGCAAGATGACCTGATCGAACCGAACCGAACCACTTCTTATAACTAAATTTGAGTTCCTCAAAATATATTTTAAGAAAAAATAACTATTTTGA
TARGET=407,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1490
PRIMER_LEFT_PENALTY=0.124655
PRIMER_RIGHT_PENALTY=0.024337
PRIMER_LEFT_SEQUENCE=TTGAACTGACCCTAGCATCTCA
PRIMER_RIGHT_SEQUENCE=GCATTTAGCACGAGTACTGCAC
PRIMER_LEFT=356,22
PRIMER_RIGHT=628,22
PRIMER_LEFT_TM=59.875
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=273
PRIMER_PAIR_PENALTY_1=0.2333
PRIMER_LEFT_1_PENALTY=0.124655
PRIMER_RIGHT_1_PENALTY=0.108684
PRIMER_LEFT_1_SEQUENCE=TTGAACTGACCCTAGCATCTCA
PRIMER_RIGHT_1_SEQUENCE=TTTGTGGTGGTCCTATCTGTTG
PRIMER_LEFT_1=356,22
PRIMER_RIGHT_1=556,22
PRIMER_LEFT_1_TM=59.875
PRIMER_RIGHT_1_TM=59.891
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.3947
PRIMER_LEFT_2_PENALTY=0.124655
PRIMER_RIGHT_2_PENALTY=0.269998
PRIMER_LEFT_2_SEQUENCE=TTGAACTGACCCTAGCATCTCA
PRIMER_RIGHT_2_SEQUENCE=TAGCACGAGTACTGCACAGACA
PRIMER_LEFT_2=356,22
PRIMER_RIGHT_2=623,22
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=59.730
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.6098
PRIMER_LEFT_3_PENALTY=0.124655
PRIMER_RIGHT_3_PENALTY=0.485189
PRIMER_LEFT_3_SEQUENCE=TTGAACTGACCCTAGCATCTCA
PRIMER_RIGHT_3_SEQUENCE=CTGTTTGTGGTGGTCCTATCTG
PRIMER_LEFT_3=356,22
PRIMER_RIGHT_3=559,22
PRIMER_LEFT_3_TM=59.875
PRIMER_RIGHT_3_TM=59.515
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.7048
PRIMER_LEFT_4_PENALTY=0.124655
PRIMER_RIGHT_4_PENALTY=0.580175
PRIMER_LEFT_4_SEQUENCE=TTGAACTGACCCTAGCATCTCA
PRIMER_RIGHT_4_SEQUENCE=ACAACTGTTTGTGGTGGTCCTA
PRIMER_LEFT_4=356,22
PRIMER_RIGHT_4=563,22
PRIMER_LEFT_4_TM=59.875
PRIMER_RIGHT_4_TM=59.420
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_05438 Ps_contig_mira-and-tgicl-ass_franssen_37645
SEQUENCE=CGAGAAAACGAATAGTTGTAAATTCAAATTTTCTTGACAACATATCAAAATATTAATCTATTTGAACTATACTTACAAAGCCAGATAAATACATTTTACCTATTACATCTGCGACTGGATTAGCTACTAACTAAGTATATAAAAATACACAATCTAAAACCGTTTATCTCTTATGTTCCATGGTTGCTTCACAGGCTTCACATATAGTTGACAGAGACTGCAACCAAGTGAGTACAAAAGAGGGACCCTTTTCATAAATAATAATAACCATACCTTGTTTTGGAGTTGGTTCTAAAATCTCTCTTCGATCATAAATGTTCACAAATCACATAATAGTTACTTAGCATCAACGTACATTGAACTGACCCTAGCATCTCATCAATTAAATTCTTCAAGACAGTAGAAACAAACCAAACCAAACCAAAACCTAGGTTAAGATAAACCAAACTACTAGAAACCAATTATTTAAAATTCAAAGATATTGTTATAAAAAATGAAATAGTGCAGAAGACAAGTTGAGTAGATTGCGACAGTACAACAGATAGGACCACCACAAACAGTTGTCTATGGAAGAAAGTAACAAATGAAACCGTTTCAAATTTTGTCTGTGCAGTACTCGTGCTAAATGCATCCCAAAAAAGTTAGATAAAGGTTGTTGAGATTACGAGACAAATAATCAGTACCACTTAAAAAGAAGAAAAGAAAATGGATTTATACTTTGTCTTGCATTAATGTCCATATATATTTCTTCTTCTGTTATATGTATATATACACACATAAATTATGTATTAATTATTAATAGTAATAAACAAATACGTGCATGTGATTGTGAATGTGTGTAGCTGGTATAAAGTAATTTATAATTAATGACAAGAGTTAAAGCACATGTATTTGCACATGTAAATTTCAAAATATTTCATAAATAAAACTTGTATAAATCTATAGGATAAGGATTAGAGATTTATTTACCTGAAATTCAACAGATCGAAATGGAGGAAGTTAGTTATCTGTTGATAATTGAGTGATTGAAGCTGCAAGATGACCTGATCGAACCGAACCGAACCACTTCTTATAACTAAATTTGAGTTCCTCAAAATATATTTTAAGAAAAAATAACTATTTTGA
TARGET=1049,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05439 Ps_contig_mira-and-tgicl-ass_franssen_37646
SEQUENCE=GTCGGGGTAAAATTGTAGTAAGTAAGTNAGTCGGTTGCCAGCTGTGCAACCACTTTGCTTTCTTCTTCTCCTTCTTCTCCCTTCCACTCCTATTTCCTTATTCTTCTTTCTTAGGGTTTTCAATTTTCACTCTCCCCTCTCCTAACATAACTCGCTTCTACAATCACTCAATCCAACAACCAAATCATCATCATCATCACACTTTCTTTCGGATTTTTTNGGTGAATCACTTGTGTTGCTAGTGGAGTTTAATATTGGTGTCTGTTTGATATGCCGGTAGTCTTTGAAATGCAATTGTGACTTGCTTTGAAAGCATATACTGGAGTGTGTAAATACTGACTTAGTTGGTTAACTAAAATAGGTTGTATTTTAAGCATTTGGGGGCTGGGTTTTATTGTGGTATTCTGAAAAAAATAGCTAAAACGGGGTCACTTTGTTGTGTGGCTTCACGGCCACATGGATCAAGTGCAGACAGTAGGGACTGGTCATTGGGTTCGCATGAGCCTTACTGGAGAACTAACACGAGCTATTCACCACCTCCATCGAGGTGGGATTTTAGATTCCAGTCTGAAGGGTTGCCTTATAATTTGAATGATCAGCTCTATGACGGGTCTTCTACATCATCAGATGGTAAAGAAAGTATGACTTTGGTCAGAGGCAACCATCTATATGATCTTCACTATTCCGTGTCAGATGGAACTGGAATTTTACTTAGCAGTTGTCCGTCTGACCTTTCTCAGGGTCCTCAGTGGGTGCCTCCAGCAATACAAGAAATAAGTATTGATGATTATGAGGCTGTGATCAGAAAAGATCCTCGTCCTTTAGGCCGGCTCTCTTTTACACCTGCCAAGGAGGGAACTTCCGAAAATCCCAACAATAGAGGCTCCACTTCAACCCAATCAGAAAG
TARGET=184,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0566
PRIMER_LEFT_PENALTY=0.038744
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=CTCCCTTCCACTCCTATTTCCT
PRIMER_RIGHT_SEQUENCE=CTACCGGCATATCAAACAGACA
PRIMER_LEFT=76,22
PRIMER_RIGHT=279,22
PRIMER_LEFT_TM=59.961
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=204
PRIMER_PAIR_PENALTY_1=0.0842
PRIMER_LEFT_1_PENALTY=0.066394
PRIMER_RIGHT_1_PENALTY=0.017846
PRIMER_LEFT_1_SEQUENCE=GCAACCACTTTGCTTTCTTCTT
PRIMER_RIGHT_1_SEQUENCE=CTACCGGCATATCAAACAGACA
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=279,22
PRIMER_LEFT_1_TM=59.934
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.1497
PRIMER_LEFT_2_PENALTY=0.038744
PRIMER_RIGHT_2_PENALTY=0.110942
PRIMER_LEFT_2_SEQUENCE=CTCCCTTCCACTCCTATTTCCT
PRIMER_RIGHT_2_SEQUENCE=TACCGGCATATCAAACAGACAC
PRIMER_LEFT_2=76,22
PRIMER_RIGHT_2=278,22
PRIMER_LEFT_2_TM=59.961
PRIMER_RIGHT_2_TM=59.889
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.1773
PRIMER_LEFT_3_PENALTY=0.066394
PRIMER_RIGHT_3_PENALTY=0.110942
PRIMER_LEFT_3_SEQUENCE=GCAACCACTTTGCTTTCTTCTT
PRIMER_RIGHT_3_SEQUENCE=TACCGGCATATCAAACAGACAC
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=278,22
PRIMER_LEFT_3_TM=59.934
PRIMER_RIGHT_3_TM=59.889
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.2568
PRIMER_LEFT_4_PENALTY=0.238974
PRIMER_RIGHT_4_PENALTY=0.017846
PRIMER_LEFT_4_SEQUENCE=TTCACTCTCCCCTCTCCTAACA
PRIMER_RIGHT_4_SEQUENCE=CTACCGGCATATCAAACAGACA
PRIMER_LEFT_4=125,22
PRIMER_RIGHT_4=279,22
PRIMER_LEFT_4_TM=60.239
PRIMER_RIGHT_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_05440 Ps_contig_mira-and-tgicl-ass_franssen_37651
SEQUENCE=ACTACTCATCAATCAATCAGAAGTTCCACTTTCTATGAAAGGAATAATAATAAAAGTTGAGTGGACTTACATTTATGCTTATGGGTCTTCTTGGAGCAACTCTTGTGCCTCACAACTTTTACCTTCATTCCTCCATTGCACAGCGGCATCAGGGACCGTAAAACATTTATAGGGATGCATTGTGTCATAATCACTTTTTTGGCCATCTTATGTGTCTTCCGTGGCCTTCATTTGGTAAATGATAGGCTG
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05441 Ps_contig_mira-and-tgicl-ass_franssen_37656
SEQUENCE=ATTNGANTANAGTACTCGGGGGCAAATTTGCATTGTAAAGAAACACAGTTCATACAACAAAAACATGGCCGATGAAAGAGAAGAAGAAGAAAGGAAAATGATAAGAATGGAAGGAGTAGCATCAATTGCATTGCTTCCAAGTGGTTCAATCTCAGGACACTTCATTCATCTTCCTCAGAACACTTGTTATGGTCTTCATGGCACTGAATTGGAATGCGAAAGGGAATGCAGTAAGGGTGAGGATTATCGCCTCGTGAAACTCACAATCACAGATTTCAATACGAAGAAAGAACAAACCACTGTTGTAGAGTGCAAAGGCCACGACGCTGCTCGGTTCCACACCATTGATCATGCTCACGGATGGGATAAAGAGATCACAGGTATGGTTGAACAAAAAGACGGAAAGAAAAGAATCACAGTTTCATTCGAGTGCGAGACACTGAAAGCCGAGAAAGCAGCTGAAGACCACATCAGGCAGTTCATGCCAAAACTAACCGGAATGGATGCTGTTGTTAACATTGGAAAAATGACGATTTTTGGGTTGGACTTTGGAGCCGAGGAAGAAGAAACCGAGTAGAAAGTTTGATATTGTAACTAGTTAGTATCCAATTAGGTTACCGAGAGAAAAACTAAATTGCACGAATTCAAATTCTGCAGAAAACTATTTTGTAGATTGGCAGGAACCTCTGGATTTGTTGCCGGTAGGAAATCAAATCGTCTGATCTTGATCGAACTACGAGGACATTTTGGATCTGTTTTTTTTTCAAAAAGTNTTTGTTCAAAATTATATTATATAGGACTTGAGACTTAAGAAAGG
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0310
PRIMER_LEFT_PENALTY=2.030986
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=TACAACAAAAACATGGCCGA
PRIMER_RIGHT_SEQUENCE=TCAGTGCCATGAAGACCATAAC
PRIMER_LEFT=52,20
PRIMER_RIGHT=206,22
PRIMER_LEFT_TM=59.969
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=40.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=2.0444
PRIMER_LEFT_1_PENALTY=2.030986
PRIMER_RIGHT_1_PENALTY=0.013401
PRIMER_LEFT_1_SEQUENCE=TACAACAAAAACATGGCCGA
PRIMER_RIGHT_1_SEQUENCE=GCGATAATCCTCACCCTTACTG
PRIMER_LEFT_1=52,20
PRIMER_RIGHT_1=249,22
PRIMER_LEFT_1_TM=59.969
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=2.0444
PRIMER_LEFT_2_PENALTY=2.030986
PRIMER_RIGHT_2_PENALTY=0.013401
PRIMER_LEFT_2_SEQUENCE=TACAACAAAAACATGGCCGA
PRIMER_RIGHT_2_SEQUENCE=CGATAATCCTCACCCTTACTGC
PRIMER_LEFT_2=52,20
PRIMER_RIGHT_2=248,22
PRIMER_LEFT_2_TM=59.969
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=197
PRIMER_PAIR_PENALTY_3=2.0620
PRIMER_LEFT_3_PENALTY=2.061961
PRIMER_RIGHT_3_PENALTY=0.000037
PRIMER_LEFT_3_SEQUENCE=AAAAACATGGCCGATGAAAG
PRIMER_RIGHT_3_SEQUENCE=TCAGTGCCATGAAGACCATAAC
PRIMER_LEFT_3=58,20
PRIMER_RIGHT_3=206,22
PRIMER_LEFT_3_TM=59.938
PRIMER_RIGHT_3_TM=60.000
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=2.0754
PRIMER_LEFT_4_PENALTY=2.061961
PRIMER_RIGHT_4_PENALTY=0.013401
PRIMER_LEFT_4_SEQUENCE=AAAAACATGGCCGATGAAAG
PRIMER_RIGHT_4_SEQUENCE=GCGATAATCCTCACCCTTACTG
PRIMER_LEFT_4=58,20
PRIMER_RIGHT_4=249,22
PRIMER_LEFT_4_TM=59.938
PRIMER_RIGHT_4_TM=59.987
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_05442 Ps_contig_mira-and-tgicl-ass_franssen_37663
SEQUENCE=TTTCTTAGGACAATGAGAGTGAGGCGTTCTCAAGGACCCACTTTTTTTAAATTTTACTAACAACAACCTGTTGTATTTTGTACACATGGCATCGTTATTACAAACAAACAACAACAAAACTCATCGTCCTCTCTTCATGATTTCAACATTTTCTCTTCTTCTCAGAAAACAAATTATAAACAATTTGCAACTTTTCCTTTCCAACAATAAAAACCATGATCAAACTCATATTTCAGAGCAAATATAATAATAGGGAAATTCACAAAGATTAATTCAAAAAANACATAAAATAAGAACAAGATTCATGCTTGCATCCTTACAGCCATTCATGAGTATTTGTTCATATTTCGAAAGCCACATAAGTAATATCCAGATATTAGCATTAGTAAAATTGTTTTCAATTTCCAATCTGAGATGAGACAGAGAAATTGTTTTTTTAAAAGACAAAAACAAACACCTTACCCAATAGCTTAGCCTAACCATCTTGTAGTAACAGCGCCGACGGAATTCGTTTGTGGCGACCGAGGTGATGTCGCTGGCGATTATCATTCCGGTGCATGTTGTTTGCTCGTCATAACCGGTTTAGGCGGAAGAGTGATGTGACGACGCGTGAGAGAAGGTTTTAAATGGCGGTCCG
TARGET=100,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0134
PRIMER_LEFT_PENALTY=0.826784
PRIMER_RIGHT_PENALTY=0.186600
PRIMER_LEFT_SEQUENCE=GACAATGAGAGTGAGGCGTTCT
PRIMER_RIGHT_SEQUENCE=TGAAATCATGAAGAGAGGACGA
PRIMER_LEFT=8,22
PRIMER_RIGHT=144,22
PRIMER_LEFT_TM=60.827
PRIMER_RIGHT_TM=59.813
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=1.1382
PRIMER_LEFT_1_PENALTY=0.951551
PRIMER_RIGHT_1_PENALTY=0.186600
PRIMER_LEFT_1_SEQUENCE=ATGAGAGTGAGGCGTTCTCAAG
PRIMER_RIGHT_1_SEQUENCE=TGAAATCATGAAGAGAGGACGA
PRIMER_LEFT_1=12,22
PRIMER_RIGHT_1=144,22
PRIMER_LEFT_1_TM=60.952
PRIMER_RIGHT_1_TM=59.813
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=1.1912
PRIMER_LEFT_2_PENALTY=1.004600
PRIMER_RIGHT_2_PENALTY=0.186600
PRIMER_LEFT_2_SEQUENCE=ATGAGAGTGAGGCGTTCTCAA
PRIMER_RIGHT_2_SEQUENCE=TGAAATCATGAAGAGAGGACGA
PRIMER_LEFT_2=12,21
PRIMER_RIGHT_2=144,22
PRIMER_LEFT_2_TM=60.005
PRIMER_RIGHT_2_TM=59.813
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=133
PRIMER_PAIR_PENALTY_3=1.2872
PRIMER_LEFT_3_PENALTY=1.100593
PRIMER_RIGHT_3_PENALTY=0.186600
PRIMER_LEFT_3_SEQUENCE=TCTTAGGACAATGAGAGTGAGGC
PRIMER_RIGHT_3_SEQUENCE=TGAAATCATGAAGAGAGGACGA
PRIMER_LEFT_3=2,23
PRIMER_RIGHT_3=144,22
PRIMER_LEFT_3_TM=59.899
PRIMER_RIGHT_3_TM=59.813
PRIMER_LEFT_3_GC_PERCENT=47.826
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=1.3208
PRIMER_LEFT_4_PENALTY=1.134222
PRIMER_RIGHT_4_PENALTY=0.186600
PRIMER_LEFT_4_SEQUENCE=GACAATGAGAGTGAGGCGTTC
PRIMER_RIGHT_4_SEQUENCE=TGAAATCATGAAGAGAGGACGA
PRIMER_LEFT_4=8,21
PRIMER_RIGHT_4=144,22
PRIMER_LEFT_4_TM=59.866
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_05443 Ps_contig_mira-and-tgicl-ass_franssen_37666
SEQUENCE=TTTACTTTTAGTTTTCCTACACTCAATTTCATAAACCTAAGTTTTTTTTCTTTCTCATTCCCAATATTTTTCTAGGGTTTCACTTTCATTTCATTTCATTCACAACCATGGGTGGTGAGTGGCATGATTGGCAATCTCTTCTTCAATCCATTTTCATCGGTCTTATTTTCTCTTACTTTCTCGCTAAGCTCATATCCATCGTCGTTTCTTTCAACAACGACAATCTCACCATCACCCGCAACACTAACATCGCCGCCACAGAACACCACGACGATGCTTCCAAACACGACGACGTCGTTTCCGATACCGGACACCGTTTCGAAACTGCTGAACGTGAGTCGGTCATGGCGGAGCAAGGTAGCATCAGAAACGAGAGCGTTGACGGTGGTGATGAGGATGACTATGACGACGATTGGGAAGGTGTTGAGAGTACTGAATTGGATGAAGCTTTCAGCGCCGCTACTGCTTTTGTTGCTGCCGCCGCCGCCGATCGTCTCTCTCAGAAGGTTTCTAATGATATTCAGCTTCAGCTTTACGGGTTGTATAAAATTGCCACTGAAGGTCCGTGTAGCACGCCGCAGCCTTCTCCCCTCAAAATGACGGCTCGTTCCAAATGGCAAGCGTGGCAGAAATTGGGTGCTATGCCTCCGGAAGATGCAATGCAGAAGTATATTGACATTGTGTCAGAGCTATATCCTACTTGGCTTGATGGTGCACCTTTGAGAAGCAAAACCGGCGATAGTGGGGGCCCTAGTTCAGAGGCCCGGGGACCAATGGGACCAGTATTCAGCTCTTTTGTTTATGAAGAAGAATGCAGCAGTGATTCGAAAATGGATGCCATTCACGGATTTGCTAGAGAGGGAGATATGACTAATCTACTCAAGTGTGTTGAAAATGGAATTTCAGTGAATTTGAAGGACAGTGAGGGCCGGACACCATTACACTGGGCTGTGGATCGTGGTCACCTTAACGTCACAGAGCTTCTGCTCAGCAAGAATGCTGATGTAAACGCCAAGGATAATGATGGACAAACTCCTCTGCACTACGCAGTAACATGCGAGAGAGAAGCCATAGCTGAATACTTAGTGAAACATAGTGCAGACATCAATTCAAAAGACAACGAAGGCAGTTCTTCGCGTGACATCTGTAGTTCCAAGTGGCCGTTTATGCAGCATGAGGAAGAAGCAAAATGATTGTTTGCTTTGTATGTTACTACTTTTGACTAGTACATCCTTGCCCAAATCCATTCAGGATGTTCAGGATGTATGTGTCCATATAACTCAATTTTTTGGCCCTTACTCTTGGTATGTACCCTTTTCACCGTAGTCTACACCTATGCACCGTTTAGGTAATGGTTAAATGAAGTATATTATGTTTTGTTGTTACTGCAATTGATATCCAAATATGCACATGTGTTGGGTGGTTTGCCGAAAGGTAGGATAGTTTAAACGAGGTTATCATGCTAGGTTAGGGGTAATATGTTTGTGTGCGTAAAATGGAATCTAAATTTCCTCTATTTGCTAGAGAAAGGTAGGTAAG
TARGET=84,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05444 Ps_contig_mira-and-tgicl-ass_franssen_37666
SEQUENCE=TTTACTTTTAGTTTTCCTACACTCAATTTCATAAACCTAAGTTTTTTTTCTTTCTCATTCCCAATATTTTTCTAGGGTTTCACTTTCATTTCATTTCATTCACAACCATGGGTGGTGAGTGGCATGATTGGCAATCTCTTCTTCAATCCATTTTCATCGGTCTTATTTTCTCTTACTTTCTCGCTAAGCTCATATCCATCGTCGTTTCTTTCAACAACGACAATCTCACCATCACCCGCAACACTAACATCGCCGCCACAGAACACCACGACGATGCTTCCAAACACGACGACGTCGTTTCCGATACCGGACACCGTTTCGAAACTGCTGAACGTGAGTCGGTCATGGCGGAGCAAGGTAGCATCAGAAACGAGAGCGTTGACGGTGGTGATGAGGATGACTATGACGACGATTGGGAAGGTGTTGAGAGTACTGAATTGGATGAAGCTTTCAGCGCCGCTACTGCTTTTGTTGCTGCCGCCGCCGCCGATCGTCTCTCTCAGAAGGTTTCTAATGATATTCAGCTTCAGCTTTACGGGTTGTATAAAATTGCCACTGAAGGTCCGTGTAGCACGCCGCAGCCTTCTCCCCTCAAAATGACGGCTCGTTCCAAATGGCAAGCGTGGCAGAAATTGGGTGCTATGCCTCCGGAAGATGCAATGCAGAAGTATATTGACATTGTGTCAGAGCTATATCCTACTTGGCTTGATGGTGCACCTTTGAGAAGCAAAACCGGCGATAGTGGGGGCCCTAGTTCAGAGGCCCGGGGACCAATGGGACCAGTATTCAGCTCTTTTGTTTATGAAGAAGAATGCAGCAGTGATTCGAAAATGGATGCCATTCACGGATTTGCTAGAGAGGGAGATATGACTAATCTACTCAAGTGTGTTGAAAATGGAATTTCAGTGAATTTGAAGGACAGTGAGGGCCGGACACCATTACACTGGGCTGTGGATCGTGGTCACCTTAACGTCACAGAGCTTCTGCTCAGCAAGAATGCTGATGTAAACGCCAAGGATAATGATGGACAAACTCCTCTGCACTACGCAGTAACATGCGAGAGAGAAGCCATAGCTGAATACTTAGTGAAACATAGTGCAGACATCAATTCAAAAGACAACGAAGGCAGTTCTTCGCGTGACATCTGTAGTTCCAAGTGGCCGTTTATGCAGCATGAGGAAGAAGCAAAATGATTGTTTGCTTTGTATGTTACTACTTTTGACTAGTACATCCTTGCCCAAATCCATTCAGGATGTTCAGGATGTATGTGTCCATATAACTCAATTTTTTGGCCCTTACTCTTGGTATGTACCCTTTTCACCGTAGTCTACACCTATGCACCGTTTAGGTAATGGTTAAATGAAGTATATTATGTTTTGTTGTTACTGCAATTGATATCCAAATATGCACATGTGTTGGGTGGTTTGCCGAAAGGTAGGATAGTTTAAACGAGGTTATCATGCTAGGTTAGGGGTAATATGTTTGTGTGCGTAAAATGGAATCTAAATTTCCTCTATTTGCTAGAGAAAGGTAGGTAAG
TARGET=477,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0565
PRIMER_LEFT_PENALTY=0.046672
PRIMER_RIGHT_PENALTY=0.009871
PRIMER_LEFT_SEQUENCE=AGGTAGCATCAGAAACGAGAGC
PRIMER_RIGHT_SEQUENCE=GTGGCAATTTTATACAACCCGT
PRIMER_LEFT=355,22
PRIMER_RIGHT=555,22
PRIMER_LEFT_TM=60.047
PRIMER_RIGHT_TM=60.010
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=11.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=201
PRIMER_PAIR_PENALTY_1=0.1746
PRIMER_LEFT_1_PENALTY=0.046672
PRIMER_RIGHT_1_PENALTY=0.127927
PRIMER_LEFT_1_SEQUENCE=AGGTAGCATCAGAAACGAGAGC
PRIMER_RIGHT_1_SEQUENCE=AGTGGCAATTTTATACAACCCG
PRIMER_LEFT_1=355,22
PRIMER_RIGHT_1=556,22
PRIMER_LEFT_1_TM=60.047
PRIMER_RIGHT_1_TM=60.128
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=11.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.2795
PRIMER_LEFT_2_PENALTY=0.269585
PRIMER_RIGHT_2_PENALTY=0.009871
PRIMER_LEFT_2_SEQUENCE=GGAGCAAGGTAGCATCAGAAAC
PRIMER_RIGHT_2_SEQUENCE=GTGGCAATTTTATACAACCCGT
PRIMER_LEFT_2=349,22
PRIMER_RIGHT_2=555,22
PRIMER_LEFT_2_TM=60.270
PRIMER_RIGHT_2_TM=60.010
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=11.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=0.3975
PRIMER_LEFT_3_PENALTY=0.269585
PRIMER_RIGHT_3_PENALTY=0.127927
PRIMER_LEFT_3_SEQUENCE=GGAGCAAGGTAGCATCAGAAAC
PRIMER_RIGHT_3_SEQUENCE=AGTGGCAATTTTATACAACCCG
PRIMER_LEFT_3=349,22
PRIMER_RIGHT_3=556,22
PRIMER_LEFT_3_TM=60.270
PRIMER_RIGHT_3_TM=60.128
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=11.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.4305
PRIMER_LEFT_4_PENALTY=0.420643
PRIMER_RIGHT_4_PENALTY=0.009871
PRIMER_LEFT_4_SEQUENCE=TGAGGATGACTATGACGACGAT
PRIMER_RIGHT_4_SEQUENCE=GTGGCAATTTTATACAACCCGT
PRIMER_LEFT_4=391,22
PRIMER_RIGHT_4=555,22
PRIMER_LEFT_4_TM=59.579
PRIMER_RIGHT_4_TM=60.010
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.0000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_05445 Ps_contig_mira-and-tgicl-ass_franssen_37671
SEQUENCE=AAGAAAGGATATGTTTATTGCTACCCTTTCAATCATGTAAGGAACAGAGCACTTTTCATTGTAAAAAGAAAACAGATCTAGCACCGAGGCAACCAACTCGGTGAGTGAGTGACCAACTCGCCGCCGTAAAATAAACTGAAATACCACCGGAATCAACCGATTTCTTTCCGAAAATTCTCTTCTTCTGCTTCTTCCTCTTGAAAACAACATTGATGTTTTTCAACGATGACATAGAGTTATAATCACACCACTCTGCAACACAAACCCTAATCCCAAATTGATTTAGTACAATCACCACTCTCACGCTTCACCATTGTGTTCGTTCGCAAGAACCTACTTCACGCTTCAGATTCACTTTCCAGATTTAGTTTGCATCTTCACAA
TARGET=101,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2961
PRIMER_LEFT_PENALTY=0.261998
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=GAAAACAGATCTAGCACCGAGG
PRIMER_RIGHT_SEQUENCE=GCAGAAGAAGAGAATTTTCGGA
PRIMER_LEFT=67,22
PRIMER_RIGHT=187,22
PRIMER_LEFT_TM=60.262
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.4591
PRIMER_LEFT_1_PENALTY=0.261998
PRIMER_RIGHT_1_PENALTY=0.197140
PRIMER_LEFT_1_SEQUENCE=GAAAACAGATCTAGCACCGAGG
PRIMER_RIGHT_1_SEQUENCE=AAGCGTGAGAGTGGTGATTGTA
PRIMER_LEFT_1=67,22
PRIMER_RIGHT_1=307,22
PRIMER_LEFT_1_TM=60.262
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.4653
PRIMER_LEFT_2_PENALTY=0.261998
PRIMER_RIGHT_2_PENALTY=0.203343
PRIMER_LEFT_2_SEQUENCE=GAAAACAGATCTAGCACCGAGG
PRIMER_RIGHT_2_SEQUENCE=TTAGGGTTTGTGTTGCAGAGTG
PRIMER_LEFT_2=67,22
PRIMER_RIGHT_2=269,22
PRIMER_LEFT_2_TM=60.262
PRIMER_RIGHT_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.6945
PRIMER_LEFT_3_PENALTY=0.261998
PRIMER_RIGHT_3_PENALTY=0.432475
PRIMER_LEFT_3_SEQUENCE=GAAAACAGATCTAGCACCGAGG
PRIMER_RIGHT_3_SEQUENCE=GAAGCGTGAAGTAGGTTCTTGC
PRIMER_LEFT_3=67,22
PRIMER_RIGHT_3=346,22
PRIMER_LEFT_3_TM=60.262
PRIMER_RIGHT_3_TM=60.432
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.7049
PRIMER_LEFT_4_PENALTY=0.261998
PRIMER_RIGHT_4_PENALTY=0.442900
PRIMER_LEFT_4_SEQUENCE=GAAAACAGATCTAGCACCGAGG
PRIMER_RIGHT_4_SEQUENCE=TGAAGCGTGAAGTAGGTTCTTG
PRIMER_LEFT_4=67,22
PRIMER_RIGHT_4=347,22
PRIMER_LEFT_4_TM=60.262
PRIMER_RIGHT_4_TM=59.557
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_05446 Ps_contig_mira-and-tgicl-ass_franssen_37688
SEQUENCE=AAGTTCGNRTGGGACTTGGATGTGGATATGATCAGATTTTTCCAGAATCCTCCTACCACCCTGTAGATGGTATGCTTGGTCTTGGCAGGGGAAAATCTAGCTTGATATCACAGTTGAGTAGTCAGGGTTTGGTGAAGTAAATTGGTTGGTTGGACACTGTTTGAGTGCACAAGGAGGAGATACATTCTTCTTTGGATATTGTTTANGATTCGTCTAACGATTGGCTTGGACT
TARGET=142,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05447 Ps_contig_mira-and-tgicl-ass_franssen_37712
SEQUENCE=AGCACATAAAACRNTYACNCNNGGGCCTCTACCCTAAAACCCTAATTCACTGCTAAACCCATTTCAACGGCGAGGCCATGACGCTATCCACGGCGATATCTCCATCCTTAACCGACTCCAAGAAGAAGAAGAACCGCAACAAAAATGGAGCAGAAGAAGATGAATCAACGGAGCAACAAAACCAGCAAGACTTCATGATCAAACCAGGCTCAAGAGCGTCGTCAATCGACACTTCAGAGTGGCCGATACTCCTCAAGAACTACGACCGCCTCAACATCCGAACCGGTCACTAYACTCCTCTTCCCGCCGGACACTCTCCGCTGAAACGGCCACTCAACGATTATCTCAAATACGGTGTCCTCAACCTTGATAAGCCAGCTAATCCTTCTTCTCACGAAGTTGTTGCTTGGATCAAGAGGATTCTCCGTGTCGAAAAAACCGGTCACAGTGGTACTCTCGATCCCAAAGTTACTGGGAATTTGATCGTTTGCATTGACAGAGCAACAAGGCTTGTTAAATCTCAACAAGGTGCTGGGAAAGAGTATGTTTGTATTGCAAGACTTCATTCAGCTGTTCCTGATGTTGCTAAGGTAGCTCGTGCTCTTGAAACCCTAACTGGTGCTGTTTTTCAGAGACCACCTTTGATTTCTGCTGTTAAGCGTCAACTTAGGATTAGAACTGTTTATGAAAGTAAGATGCTTGAATATGATGCCGAGAGACATTTAGTCGTGTTTTGGATTTCATGTGAAGCTGGTACTTACGTTAGGACTATGTGTGTTCATTTGGGTTTATTGCTTGGTGTTGGTAGTCATATGCAAGAACTTAGGAGGGTTAGGTCTGGGATTATGGGTGAGAAAGACAATATGGTTTCTATGCATGATGTTATGGATGCTCAATGGGTTTATGATAATAATCGTGACGAGACTTATTTGAGGAGGGTTGTTATGCCTTTGGAAGTTCTTTTGACTAGTTATAAGAGATTAGTTGTTAAGGATTCGGCTGTTAATGCTATTTGTTATGGGGCTAAGTTGATGATTCCGGGTTTGCTTAGGTTTGAGAATGATATTGATGCTGGCGAGGAGGTTGTGCTTATGACGACCAAGGGTGAAGCTATTGCCCTTGGGATTGCTGAGATGACTACTGCTGTTATGGCTAGTTGTGATCATGGCATTGTTGCTAAGATCAAGAGGGTGGTTATGGATAGGGATACTTATCCGAGGAAATGGGGTTTGGGTCCTAGGGCTTCTACGAAGAAGAAGCTGATTGCTCTAGGGAAGCTTGATAAGCATGGGAAGCCAAATGAGCATACTCCTGAAGAGTGGTCGAGGAATGTGGTGGCATTGCCTGCTGGTTGGGACAGCGCGGTTGCTGCAAAGGCTGCTGCTGCTACTGAAACTGAAGATGGGAAGAAAGCGGTTGCTGAAGATGGGGAAGGGCGTAAGAGAAAGAAACATGAAACTGACGACAGTCCTGCTCCTGCTAAGAAGTCCAAAGTTGCTGAAGTTGAAGATGAAGAGAAAGTGAAGACCATAAAAGTAGATGAAGCTGCTGCGGATGTTGAAGACGAGAAGAAAGAAAAGAAGAAAAAGAAAAAGAAGAAGGATAAAGAGAATGGAGATGCTGCATCTTCCGATGATGAGAAAGTTGTAAAGGAAAAGAAAAAGAAGAACAAGGAAAAGGGTGAAGATGGTTCTCCTGAAGTGGACAAGTCCGAGAAGAAGAAAAAGAAGCAAAAGGAAAAAGTTGAAGATGCTTCACCTGAAGTGGAGAAGTCTGAGAAGAAGAAAAAGAAGAAAGACAAAGAAGCCAAAGATAGTGCTGCAGAGATCAGTAACGGAAAGGAAGAAGGAAGCGCAGATAGAAGTGAAAAGAAGCACAAGAAAAAGAAAAACAAAGATGTCCAAGAAGAATAGATGATGGCGTGATTTTGTGATTTTGGTTTAACATGTAATGCCATTTCTTCTTTATTCATAGTTTACTTTGTTTAGTTTGCTGTTTTTACTTTAATGTTTTATAGTTCTCTAATTATCATGTTTTGGGTCGACCTTGTAGTACGTTATATCTTRTNAAAGGACCGTTTGTCTTAAGATTGTTCTATTTAAAGTTT
TARGET=120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05448 Ps_contig_mira-and-tgicl-ass_franssen_37712
SEQUENCE=AGCACATAAAACRNTYACNCNNGGGCCTCTACCCTAAAACCCTAATTCACTGCTAAACCCATTTCAACGGCGAGGCCATGACGCTATCCACGGCGATATCTCCATCCTTAACCGACTCCAAGAAGAAGAAGAACCGCAACAAAAATGGAGCAGAAGAAGATGAATCAACGGAGCAACAAAACCAGCAAGACTTCATGATCAAACCAGGCTCAAGAGCGTCGTCAATCGACACTTCAGAGTGGCCGATACTCCTCAAGAACTACGACCGCCTCAACATCCGAACCGGTCACTAYACTCCTCTTCCCGCCGGACACTCTCCGCTGAAACGGCCACTCAACGATTATCTCAAATACGGTGTCCTCAACCTTGATAAGCCAGCTAATCCTTCTTCTCACGAAGTTGTTGCTTGGATCAAGAGGATTCTCCGTGTCGAAAAAACCGGTCACAGTGGTACTCTCGATCCCAAAGTTACTGGGAATTTGATCGTTTGCATTGACAGAGCAACAAGGCTTGTTAAATCTCAACAAGGTGCTGGGAAAGAGTATGTTTGTATTGCAAGACTTCATTCAGCTGTTCCTGATGTTGCTAAGGTAGCTCGTGCTCTTGAAACCCTAACTGGTGCTGTTTTTCAGAGACCACCTTTGATTTCTGCTGTTAAGCGTCAACTTAGGATTAGAACTGTTTATGAAAGTAAGATGCTTGAATATGATGCCGAGAGACATTTAGTCGTGTTTTGGATTTCATGTGAAGCTGGTACTTACGTTAGGACTATGTGTGTTCATTTGGGTTTATTGCTTGGTGTTGGTAGTCATATGCAAGAACTTAGGAGGGTTAGGTCTGGGATTATGGGTGAGAAAGACAATATGGTTTCTATGCATGATGTTATGGATGCTCAATGGGTTTATGATAATAATCGTGACGAGACTTATTTGAGGAGGGTTGTTATGCCTTTGGAAGTTCTTTTGACTAGTTATAAGAGATTAGTTGTTAAGGATTCGGCTGTTAATGCTATTTGTTATGGGGCTAAGTTGATGATTCCGGGTTTGCTTAGGTTTGAGAATGATATTGATGCTGGCGAGGAGGTTGTGCTTATGACGACCAAGGGTGAAGCTATTGCCCTTGGGATTGCTGAGATGACTACTGCTGTTATGGCTAGTTGTGATCATGGCATTGTTGCTAAGATCAAGAGGGTGGTTATGGATAGGGATACTTATCCGAGGAAATGGGGTTTGGGTCCTAGGGCTTCTACGAAGAAGAAGCTGATTGCTCTAGGGAAGCTTGATAAGCATGGGAAGCCAAATGAGCATACTCCTGAAGAGTGGTCGAGGAATGTGGTGGCATTGCCTGCTGGTTGGGACAGCGCGGTTGCTGCAAAGGCTGCTGCTGCTACTGAAACTGAAGATGGGAAGAAAGCGGTTGCTGAAGATGGGGAAGGGCGTAAGAGAAAGAAACATGAAACTGACGACAGTCCTGCTCCTGCTAAGAAGTCCAAAGTTGCTGAAGTTGAAGATGAAGAGAAAGTGAAGACCATAAAAGTAGATGAAGCTGCTGCGGATGTTGAAGACGAGAAGAAAGAAAAGAAGAAAAAGAAAAAGAAGAAGGATAAAGAGAATGGAGATGCTGCATCTTCCGATGATGAGAAAGTTGTAAAGGAAAAGAAAAAGAAGAACAAGGAAAAGGGTGAAGATGGTTCTCCTGAAGTGGACAAGTCCGAGAAGAAGAAAAAGAAGCAAAAGGAAAAAGTTGAAGATGCTTCACCTGAAGTGGAGAAGTCTGAGAAGAAGAAAAAGAAGAAAGACAAAGAAGCCAAAGATAGTGCTGCAGAGATCAGTAACGGAAAGGAAGAAGGAAGCGCAGATAGAAGTGAAAAGAAGCACAAGAAAAAGAAAAACAAAGATGTCCAAGAAGAATAGATGATGGCGTGATTTTGTGATTTTGGTTTAACATGTAATGCCATTTCTTCTTTATTCATAGTTTACTTTGTTTAGTTTGCTGTTTTTACTTTAATGTTTTATAGTTCTCTAATTATCATGTTTTGGGTCGACCTTGTAGTACGTTATATCTTRTNAAAGGACCGTTTGTCTTAAGATTGTTCTATTTAAAGTTT
TARGET=1377,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05449 Ps_contig_mira-and-tgicl-ass_franssen_37767
SEQUENCE=TGGTATCAACGCAGAGTAGCAATTAATTTATTTTTGTTGAAAAAGAGAGAGAGCAATTGTGCAAAGTGATGGCTGCTGATTGGGGTCCGGTGGTGATATCAGTGGTGCTATTTGTTCTACTAAGTCCAGGGCTTCTGTTCCAGCTACCTGCTAAGGGAAGGGTGGTAGCTTTTGGAAGCATGCAAACTAGTGGTATCTCTATCTTGGTCCATACCATCATCTTCTTTGGACTCATCACCATTTTTCTACTTGCTATTGGTGTCCATATCTACTCAGGATAATCAAATCAAATCAAATCATCCCCTAAAAAAATGGCATTATTAATTGTTTTTTTTCTATCCTTGGTGTTTTCATTTATATATTCTTGTTTAAATTATGAGTTGTACTTTTACAATATGTAACTAAGGATGACGGGTGGCTATTGATCTTCAACTCACAATTATTTTTTCTAAAATTTTAATTACTCCTAATTTATTATGTTCTACTATAAGTTTGTGACTAATGATTATCAAGGTAAATTTTACAAAATTGTATATTCATTTTTTTTCCTTCATATACTTAAAAAATAAAATGAAGTTCGTT
TARGET=44,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8394
PRIMER_LEFT_PENALTY=0.821393
PRIMER_RIGHT_PENALTY=0.018048
PRIMER_LEFT_SEQUENCE=TGGTATCAACGCAGAGTAGCAA
PRIMER_RIGHT_SEQUENCE=AAAGCTACCACCCTTCCCTTAG
PRIMER_LEFT=0,22
PRIMER_RIGHT=171,22
PRIMER_LEFT_TM=60.821
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.9395
PRIMER_LEFT_1_PENALTY=0.821393
PRIMER_RIGHT_1_PENALTY=0.118120
PRIMER_LEFT_1_SEQUENCE=TGGTATCAACGCAGAGTAGCAA
PRIMER_RIGHT_1_SEQUENCE=TAGCACCACTGATATCACCACC
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=109,22
PRIMER_LEFT_1_TM=60.821
PRIMER_RIGHT_1_TM=59.882
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=110
PRIMER_PAIR_PENALTY_2=1.1659
PRIMER_LEFT_2_PENALTY=0.821393
PRIMER_RIGHT_2_PENALTY=0.344509
PRIMER_LEFT_2_SEQUENCE=TGGTATCAACGCAGAGTAGCAA
PRIMER_RIGHT_2_SEQUENCE=AAAAGCTACCACCCTTCCCTTA
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=172,22
PRIMER_LEFT_2_TM=60.821
PRIMER_RIGHT_2_TM=60.345
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=1.5277
PRIMER_LEFT_3_PENALTY=0.821393
PRIMER_RIGHT_3_PENALTY=0.706271
PRIMER_LEFT_3_SEQUENCE=TGGTATCAACGCAGAGTAGCAA
PRIMER_RIGHT_3_SEQUENCE=TACCACTAGTTTGCATGCTTCC
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=194,22
PRIMER_LEFT_3_TM=60.821
PRIMER_RIGHT_3_TM=59.294
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=195
PRIMER_PAIR_PENALTY_4=1.5897
PRIMER_LEFT_4_PENALTY=1.571701
PRIMER_RIGHT_4_PENALTY=0.018048
PRIMER_LEFT_4_SEQUENCE=GGTATCAACGCAGAGTAGCAAT
PRIMER_RIGHT_4_SEQUENCE=AAAGCTACCACCCTTCCCTTAG
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=171,22
PRIMER_LEFT_4_TM=58.428
PRIMER_RIGHT_4_TM=60.018
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_05450 Ps_contig_mira-and-tgicl-ass_franssen_37767
SEQUENCE=TGGTATCAACGCAGAGTAGCAATTAATTTATTTTTGTTGAAAAAGAGAGAGAGCAATTGTGCAAAGTGATGGCTGCTGATTGGGGTCCGGTGGTGATATCAGTGGTGCTATTTGTTCTACTAAGTCCAGGGCTTCTGTTCCAGCTACCTGCTAAGGGAAGGGTGGTAGCTTTTGGAAGCATGCAAACTAGTGGTATCTCTATCTTGGTCCATACCATCATCTTCTTTGGACTCATCACCATTTTTCTACTTGCTATTGGTGTCCATATCTACTCAGGATAATCAAATCAAATCAAATCATCCCCTAAAAAAATGGCATTATTAATTGTTTTTTTTCTATCCTTGGTGTTTTCATTTATATATTCTTGTTTAAATTATGAGTTGTACTTTTACAATATGTAACTAAGGATGACGGGTGGCTATTGATCTTCAACTCACAATTATTTTTTCTAAAATTTTAATTACTCCTAATTTATTATGTTCTACTATAAGTTTGTGACTAATGATTATCAAGGTAAATTTTACAAAATTGTATATTCATTTTTTTTCCTTCATATACTTAAAAAATAAAATGAAGTTCGTT
TARGET=280,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9492
PRIMER_LEFT_PENALTY=0.436048
PRIMER_RIGHT_PENALTY=0.513182
PRIMER_LEFT_SEQUENCE=CTGTTCCAGCTACCTGCTAAGG
PRIMER_RIGHT_SEQUENCE=AGTTGAAGATCAATAGCCACCC
PRIMER_LEFT=134,22
PRIMER_RIGHT=433,22
PRIMER_LEFT_TM=60.436
PRIMER_RIGHT_TM=59.487
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=1.1509
PRIMER_LEFT_1_PENALTY=0.018048
PRIMER_RIGHT_1_PENALTY=1.132848
PRIMER_LEFT_1_SEQUENCE=CTAAGGGAAGGGTGGTAGCTTT
PRIMER_RIGHT_1_SEQUENCE=CCACCCGTCATCCTTAGTTACA
PRIMER_LEFT_1=150,22
PRIMER_RIGHT_1=417,22
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=61.133
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=1.2195
PRIMER_LEFT_2_PENALTY=0.706271
PRIMER_RIGHT_2_PENALTY=0.513182
PRIMER_LEFT_2_SEQUENCE=GGAAGCATGCAAACTAGTGGTA
PRIMER_RIGHT_2_SEQUENCE=AGTTGAAGATCAATAGCCACCC
PRIMER_LEFT_2=173,22
PRIMER_RIGHT_2=433,22
PRIMER_LEFT_2_TM=59.294
PRIMER_RIGHT_2_TM=59.487
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=1.4774
PRIMER_LEFT_3_PENALTY=0.344509
PRIMER_RIGHT_3_PENALTY=1.132848
PRIMER_LEFT_3_SEQUENCE=TAAGGGAAGGGTGGTAGCTTTT
PRIMER_RIGHT_3_SEQUENCE=CCACCCGTCATCCTTAGTTACA
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=417,22
PRIMER_LEFT_3_TM=60.345
PRIMER_RIGHT_3_TM=61.133
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=1.5590
PRIMER_LEFT_4_PENALTY=1.045831
PRIMER_RIGHT_4_PENALTY=0.513182
PRIMER_LEFT_4_SEQUENCE=CCATCATCTTCTTTGGACTCATC
PRIMER_RIGHT_4_SEQUENCE=AGTTGAAGATCAATAGCCACCC
PRIMER_LEFT_4=213,23
PRIMER_RIGHT_4=433,22
PRIMER_LEFT_4_TM=59.954
PRIMER_RIGHT_4_TM=59.487
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_05451 Ps_contig_mira-and-tgicl-ass_franssen_37784
SEQUENCE=TTTACCCTAACCCACTGAAAAGAACCCAACTGACAAGTNGATTACATTTTCAAGGCATGCTTCTAAACCAAAGCATTCCAAACTAAAACCCTATCCATGACCAATGTCGTGACTGTTGCCTTGCCATATTACCCAACTTCAGTTTATCTTGCTGAAAAATGACCAAGACCCTGTTAAGTTAGCTGCACAAATGAAGTCCTGCACAAGCATTATACTGCAAAACACCTTTGTACCTGCAGAGCTATCAAATCGGACAAACCAACAAACGTCCTATTTTTCACCTTGTTCCACAAAATATAATAGATTAACCATTAGAAACAATTCACCATATAAATGTTAACCAATCACAACAACAATTTCAGTCTCAACTAATCAACCTAACTTCTAACTGCCAAACCAGTTACAACAAACTTCAAACTGACAACTAACTTCTCAATGGCATAAATTGCAACTAACTAAAAACTTCCTAACTGAAAATCGTAACAAACTCCTAACCAACTCAATGGCTTATGAACTAAATCAATTGAGTCACCATCCTCTATATAAATCAGAACCTCGTAATCTCAAGGCGATGATCATAATTTTTTTTCTCACAAAACTGAAACTGAAAACCTGACTTATTTCTCTCTCAATCTGAAAAATCCCAACGAATGTTCAACCAAAATCTCCATCTCAAAATCATCCATTTTCCCCCAATCTTCTTCACCTTCAACTCTCAGAGTTCATCATCCTCTCCAATTATCCATCTTCTTCAGAGTAGCCATCAAGGCACACACATAACAACAAAAAATACCAAGAACAAAAGAAGAAACGGGTTGAGGGAAGTATAAAGGCTGGAGAATTTGAAGAAGAAGAAGGATATGCATAATCATTAAATAGAGGAAGAAATAGGTACCTGAGGAGCTAGAAGATCTCTAGCTCGTGTATTAT
TARGET=845,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.8261
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=2.802588
PRIMER_LEFT_SEQUENCE=CCCAATCTTCTTCACCTTCAAC
PRIMER_RIGHT_SEQUENCE=GCTCCTCAGGTACCTATTTCTTCC
PRIMER_LEFT=691,22
PRIMER_RIGHT=903,24
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.803
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=2.8278
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=2.804285
PRIMER_LEFT_1_SEQUENCE=CCCAATCTTCTTCACCTTCAAC
PRIMER_RIGHT_1_SEQUENCE=TCCTCAGGTACCTATTTCTTCCTC
PRIMER_LEFT_1=691,22
PRIMER_RIGHT_1=901,24
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.196
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.833
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=2.8757
PRIMER_LEFT_2_PENALTY=0.073088
PRIMER_RIGHT_2_PENALTY=2.802588
PRIMER_LEFT_2_SEQUENCE=TCAAGGCACACACATAACAACA
PRIMER_RIGHT_2_SEQUENCE=GCTCCTCAGGTACCTATTTCTTCC
PRIMER_LEFT_2=763,22
PRIMER_RIGHT_2=903,24
PRIMER_LEFT_2_TM=60.073
PRIMER_RIGHT_2_TM=60.803
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=141
PRIMER_PAIR_PENALTY_3=2.8774
PRIMER_LEFT_3_PENALTY=0.073088
PRIMER_RIGHT_3_PENALTY=2.804285
PRIMER_LEFT_3_SEQUENCE=TCAAGGCACACACATAACAACA
PRIMER_RIGHT_3_SEQUENCE=TCCTCAGGTACCTATTTCTTCCTC
PRIMER_LEFT_3=763,22
PRIMER_RIGHT_3=901,24
PRIMER_LEFT_3_TM=60.073
PRIMER_RIGHT_3_TM=59.196
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.833
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=2.9995
PRIMER_LEFT_4_PENALTY=0.196903
PRIMER_RIGHT_4_PENALTY=2.802588
PRIMER_LEFT_4_SEQUENCE=TCAGAGTTCATCATCCTCTCCA
PRIMER_RIGHT_4_SEQUENCE=GCTCCTCAGGTACCTATTTCTTCC
PRIMER_LEFT_4=715,22
PRIMER_RIGHT_4=903,24
PRIMER_LEFT_4_TM=59.803
PRIMER_RIGHT_4_TM=60.803
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=189
=
PRIMER_SEQUENCE_ID=SSR_05452 Ps_contig_mira-and-tgicl-ass_franssen_37791
SEQUENCE=AATAAAACTTGGGTTTCGTTAAAGGGGGACGTAAGCTTCTATGGTTTTCATGGAGCTTTGAAGGAAGGAAGGACGTTTGGGCAGAAGTTTTTTGTAGATATAGATGCTTGGATGGATCTCAAACCAGCTGGAAAATCTGATAACTTGTCTGATTCTTTTAGTTATGTTGAGTTATATCGTATAGCTAAGGAAGTTATYGRRGGACCAGCTCAAAATCTTCTGGAGTCAGTGGCCCAAAAAAATTGCAATCTCCACTTTGGAAATTCACAAAGACATATCTGCTGTTCGTGTCAAGGTTGGAAAGCCTCATGTGGCAATTCCGGGCCCGCTTGATTACTTAGGTGTTGAGATTTTGAGACGCAGAGCTATTCAGCATTCCAGGAGTTTATGGTGTCATGGTAAGCAATGCCCAAGAGGAATTGCTGCAGTGGCACGCAGAAAATGCAAAAGATAACCCTAAGATTCTGCATGTCGTTCGGAGCGCTGTGCATCGTGGTATTACTAACAAGCTAGTACGGTACTTAATTTTAAAGTTCCGGACCCTAAC
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05453 Ps_contig_mira-and-tgicl-ass_franssen_37800
SEQUENCE=ACACATTGCCTATAATATACGAGCGTGGATTAATCGGTATTTATCACGATTGGTGCGAGTCGTTTAGCACCTATCCTCGATCTTATGATCTTCTCAATGCTGATTCTTTATTCTCAACCCTTAAAGAAAGGTGCAACATAGTGGCCGCGATTGCAGAAGTTGATAGAATGTTGAGACCAGAAGGATATTTGATAATAAGAGACAATGAAGAAACAATTGGTGAGATAGAGAACATGGCAAAATCTCTACATTGGGATATTCGTTTTAGTTATGCAAAAAATGGAGAGGGTTTACTTTGTCTTCAGAAGACATTTTGGAGACCAACGAAGGTTGAAACTATTGTGTCAGCCATTGCTTAATTTATGATGCATCACCTTCACTCACTTTCCACTCATTGTTTTGACAGAATTGTTATTTTCTTATTCTTTACTTCTATGCTTATTTAAGAATGTCACTTGTACAACAACTCTGTAATATAATATTTATTTATTATTATAATGTAAATATATGTGGTTTAATTTTTAAACG
TARGET=480,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05454 Ps_contig_mira-and-tgicl-ass_franssen_37806
SEQUENCE=TCACAATATAATAATTTATCACAATAATACCTTTTTATTTATTTATTTAGTTGATTTGAATAATTGACTTCCAAGTGTTACAGTTGGTGTTCCTTCACCCATCATTGTTGTTAAGTTCATCTCTTTTTCGAGGATTCGGTCGAGTTCCGTTTCGACGTAGCTCATCGAAGGTCTTCTTTCGCTTGAAGGATCCAAACATCTTATTAAGAGTAGAATGTAGTTTTCCATGCCTTCAGCTGTGAATCTACTTTCCATTCTTTGATCTATGATTGCAGACATGGTGCTACTGTCTGGATTACTTAGCACCGATTCGACCAGATTTTGACTTGTGTTTATAGAGGGTGATTCTGTTGCTTCCTTCCCACTCAACAATTCCAACAAAAATACTCCAAAGCTATACACATCGCTCTTCTCAGAAAATTGTCGGAACTCTCTCACCTCTGGTGCAAGAAATATCTCATCTGACGCCACTTGTGAAGACGAGCCTACAATTTCAACTCTCCCTAGAAAGTTACGAAGTCCTGCATCCGCGACCTTAGAAATGAAGTTTTCATCGACAAGAACATTAGATGTTTTGAAATTCTTATGCACCAAACGCGGACTCAAAGAATGAAGATGAGCCAAACCTTTAG
TARGET=34,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05455 Ps_contig_mira-and-tgicl-ass_franssen_37807
SEQUENCE=CGTTCCAGAAAGGACATGGTAGTCAACGCAGAGTACGTCGGGARGCACTCAAAGAACATTATTAAGGTTTTACTATTCCCCTTAATTCCCTATTTCTAACTAAGCCATAATATTCACACACGTTTCTCAATCAAATTCTTCAAACAAGAGATTGATTGAAAACAAAAAATGTGCCCTCTGAGGGTTCTTCTGATTTTCTTGTCCGCAACTCTCGCCGGATTCTTCGTTCTCAGAAATTTAAGATCTCAACCAAAGATCGATGATGATGAAGTTGCTGATTCAACTCTTCCATCAAACCCTAAAATCTTAGATTCTTCAAATGCTGCATCGGATGGAAATTCCAAGGTTTGGAATGCGTTAGAATCTGGGTTTTGGACCTTTGTTGATATGGCTAGTGGCCGTTATCTTTGGAGGCACGTGGTTTCATCATCTTCTTCAAAGCGCTCCAGCTGATGATGTGTTGTTGGTGATTGCAAATTTGCATATCGGGTGATTGATCAATATCAATGTCATTAGTCTTTGATATTGTAATATTTGCAGCAATTTGCACATCTTTGTAATCTGTGTATCATATCTGTTGTTCATAGTATTTTGTTGTTGGTTTTGAATTTGCAATACAAATAATGATGATGATGATGATGATGATGATAATGATGATCTTGATAGATGTCAAATATATAGATAGTGTGTACTTTACTAGTACTRTTGTGGATTTGAGTATTGTTTTCTTTGTTTTCA
TARGET=624,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05456 Ps_contig_mira-and-tgicl-ass_franssen_37815
SEQUENCE=CTGAAAAACGTGACCAGTCTTTTTTTTATAGCATGAAATGAACCATAATTTCAAGAAGCTACAAATGTAATACTAACAAACAAACTTCATAATCTGTTTCAAGAAACTAAGTTTAGGAAGAGTAAGTAATATGCAAATATTTCTATTCAGAATCACTAAAATCATTATGTCTTTAAGGTAAAAGCTGTACATAACCAGTTGATATAGCTATTCCTAGTATAATAAGAGGTGGAAGAATGACAAAAGCTCCTATAGTTGCAAAAAAAGTTGAATCATTTCTTTGAGAAACCTCATTTAGATAATTCTGTCTCTTGATATTTCTCCTTTGTGAAGTTGTTAAGAATTTTGCACTCACTTTCTTCAAGTTCTTACCTAAGGGAATGGTGAAATCATCGTCCCTATCTCCAACCAACTGCGCAAGTTGGTCGCGAATTGAAAGTTTCCGAGAGCCGCCAGCGGTTTCATTACCATCGATTGATGAGGTTGTAGATGTAAATTGCTCCAAGAGATCCAGCTGTGCACGAGAAGAAGATAAAGATCCAAGAGAAGAATCGTCCTCAGAAACACCAGCGTCAGTTCCTTGTGATTGAGCACATATCTTGTTGTTTATTAAAGAAGAAGAAGAAGGAGGGTGATTGAATTTGACGTTATGTTTGTGTTTGAGAAAGGAAGGGTTTGAAGGAAGAGAAGAAGTTGTGCTGAGATTCAGATGGATTGCCATGAGAAATCAGAAGAATTATTGCTGAGTTTGATAATTGGCATTGATTTTTGGTTTGAGAGTTTCCCCCNNYGTRTAATTTTTTTGGGGTTTATATGAATCAAGCAAAGGTCCGG
TARGET=613,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05457 Ps_contig_mira-and-tgicl-ass_franssen_37836
SEQUENCE=TCGGGAAGATGGAGAAGGAAAAAGAAGGTTTCCCACTGACTTCTCTTAGGGAAATAAATGTTCTTCTCTCTTTCACCACCCATACATAGTTGATGTCAAGGAAGTAGTGGTTGGAAGTAGCCTTGATAGTATTTTCATGGTTATGGAATACATGGAACATGATCTTAAAGGATTAATGGAGTCAATGAAGCAGCCGTTTAGCCAAAGTGAAGTGAAGTGCTTAATGATCCAACTTTTAGAAGGTGTGAAGTATCTTCATGACAACTGGGTACTTCATAGGGATTTGAAGACTTCAAACCTGCTTTTAAATAACAGGGGTGAGCTAAAAATTTGTGATTTTGGGTTGGCTCGTCAATATGGGAGTCCTTTGAAACCATATACTCACCTGGTGGTTACTCTTTGGTACAGGGCACCCGAACTTCTTCTTGGGGCAAGACAGTACTCAACAGCTATTGATATGTGGTCTCTTGGTTGTATAATGGCCGAACTTTTGTCTAAGGAACCACTTTTCAATGGAAAAACGGAATTTGATCAGCTTGACAAGATTTTCAGGATACTTGGCACTCCAAATGAAACAATTTGGCCGGGCTTCTCGAAATTGCCAGGAGTCAAGGTCAATTTTGTCAAGCACCAGTATAATCTTCTGCGTAAAAAGTTCCCTGCCACGTCATTCACTGGATCTCCAGTTCTTTCTGATTCTGGATTTGATTTGTTGAACAAGCTTCTAACTTATGACCCTGAAAAGAGGATCACGGCTGAAGCCGCTCTCAATCATGAATGGTTTCGTGAAGTTCCTCTTCCCAAGAGCAAAGAATTCATGCCTACATTTCCTGCTCAACATGCTCAGGACAGGCGGTTGCGTAGAATGTACAAGAGTCCAGATCCTTTAGAGGAGCAGCGGCGGAAGGAGTTGCAGCAAGGTGATTTGGGCACTGGTGGTAAATTTTTTGGGTTAARNATTGGGAGCTTATTTAAACCTNTGTCTAAACAGTCTATTCTTA
TARGET=205,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05458 Ps_contig_mira-and-tgicl-ass_franssen_37840
SEQUENCE=AGGATCCTAAATCTTTGGACTCTGAAAACAAGACAAAATTTTCTCTTTCTGCACGCATACTACATCCAATCAAAGTCAAATCCCGATGGCTGAAGTAGATTCACCACCGCCGCCTCCGCCACCGCAGTACCTAGAGGTTAACTGCATAACCTCCGGCAAGATCCTACGGTTCGCTCCGGGCACCGATGCAGGATTCGCGGTGGCGCTGATAAATCGGAAACTGAAAGGAACAGTTCCATTGGTAACGCACATAGAAGCTGTGAAAGATGGAGAAGAAGAAGAAGCAGTTGCTTTTGGACCAAACGCAATTCTTTCCAATTTTGGTGAAGATTGGAAGCTTCAAACCGTTCTTTCTTCGGAAGAACAAAGAAAAGGACCTATTCAAGGAGTGGCAAAGCAAACATTAGAACATGTTTCAAATGTCCCAGGTGGTGAAAGCAGAGAACCTAACCCAATAGGCTCAGTATATATCGTTAAAATAATATTTGCTTTTATTTTGATTTTTGTTCTTGGTGCAANTTTTTACTTTGTTTCTTGATAATCTTCCNGCATTGATTATTGTTTGTCAGTCATTTATATAATTCATTTTTCATTTTATGTTTNTAACTTAAATGTATGTAA
TARGET=271,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3113
PRIMER_LEFT_PENALTY=0.052366
PRIMER_RIGHT_PENALTY=0.258908
PRIMER_LEFT_SEQUENCE=AACGCACATAGAAGCTGTGAAA
PRIMER_RIGHT_SEQUENCE=GTCCTTTTCTTTGTTCTTCCGA
PRIMER_LEFT=243,22
PRIMER_RIGHT=376,22
PRIMER_LEFT_TM=59.948
PRIMER_RIGHT_TM=59.741
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.3995
PRIMER_LEFT_1_PENALTY=0.052366
PRIMER_RIGHT_1_PENALTY=0.347129
PRIMER_LEFT_1_SEQUENCE=AACGCACATAGAAGCTGTGAAA
PRIMER_RIGHT_1_SEQUENCE=CTTTGCCACTCCTTGAATAGGT
PRIMER_LEFT_1=243,22
PRIMER_RIGHT_1=396,22
PRIMER_LEFT_1_TM=59.948
PRIMER_RIGHT_1_TM=59.653
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=154
PRIMER_PAIR_PENALTY_2=0.4032
PRIMER_LEFT_2_PENALTY=0.165486
PRIMER_RIGHT_2_PENALTY=0.237699
PRIMER_LEFT_2_SEQUENCE=TGGTAACGCACATAGAAGCTGT
PRIMER_RIGHT_2_SEQUENCE=TTGGGTTAGGTTCTCTGCTTTC
PRIMER_LEFT_2=239,22
PRIMER_RIGHT_2=454,22
PRIMER_LEFT_2_TM=59.835
PRIMER_RIGHT_2_TM=59.762
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=216
PRIMER_PAIR_PENALTY_3=0.4032
PRIMER_LEFT_3_PENALTY=0.165486
PRIMER_RIGHT_3_PENALTY=0.237699
PRIMER_LEFT_3_SEQUENCE=GGTAACGCACATAGAAGCTGTG
PRIMER_RIGHT_3_SEQUENCE=TTGGGTTAGGTTCTCTGCTTTC
PRIMER_LEFT_3=240,22
PRIMER_RIGHT_3=454,22
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=59.762
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=0.4244
PRIMER_LEFT_4_PENALTY=0.165486
PRIMER_RIGHT_4_PENALTY=0.258908
PRIMER_LEFT_4_SEQUENCE=GGTAACGCACATAGAAGCTGTG
PRIMER_RIGHT_4_SEQUENCE=GTCCTTTTCTTTGTTCTTCCGA
PRIMER_LEFT_4=240,22
PRIMER_RIGHT_4=376,22
PRIMER_LEFT_4_TM=59.835
PRIMER_RIGHT_4_TM=59.741
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_05459 Ps_contig_mira-and-tgicl-ass_franssen_37869
SEQUENCE=ACGCGGGGGTGGTAGTGATCGTGATTCTGGGATGAGTTTAGAAACAGTTAATTCCAATACACAGAGAACTTCTTTAAGCAGCGCCACTGAAAGCATTTGCAGCACAAGTTTCAGCCGTCTCTCTTTCGAACTCCTTCCGTCATCCTCACCGGAGAGTCTCTCCATCAAACCTCACCGTTCCTCCGATTTTGCCTACTCCGCCATCCGCAAATCCGGTCTCACCTTCCGTGACTTCCACCTTCTCCGCCGCATAGGCTCCGGTGACATAGGCACAGTATACCTCTGTCGCTTACGTGACTCGTCCGTAAACTACATCAACGACGAGGATAGTTCGTTCTACTACGCCATGAAAGTTGTTGACAAAGACGCTGTTGCTCTCAAGAAGAAGTCACACAGAGCAGAAATGGAGAGAAAGATTCTCAAGATGCTTGATCATCCTTTTCTCCCTAGTCTCTATGCCGAGTTCGAAGCTTCTAATTTCTCTTGCATTGTAATGGAGTTTTGCTCCGGCGGTGACTTGCACTCTCTCCGTCACCGTCACCCTCGTAACCGTTTCTCTCTCTCCTCAGCACGGTTTTACGCGGCTGAGGTGCTTGTAGCATTGGAGTATCTGCACATGTTAGGAATAATTTACAGAGATCTAAAACCGGAAAACGTGTTAGTCAGATCAGACGGTCACATCATGCTCTCTGATTTTGATCTATCTCTTTGCTACCCACGCAATCCCAGCCGTTGAGTTATCACCAGACGATCCACCACTGGATGTATCCTGCACCCGTCCGCATTCCATTTCTTCTCCTTTCAAGTGTCTCTCGAAGCGACTTTTCCGGTCCAGAAAGGTTCAAACATTTCAATCAAACCGGCTTTTTGTCGCTGAACCCGTTGAAGCACGGTCTTGTTCGTTCGTCGGAACACACGAGTACCTAGCACCAGAA
TARGET=555,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1639
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.161579
PRIMER_LEFT_SEQUENCE=TCTCCCTAGTCTCTATGCCGAG
PRIMER_RIGHT_SEQUENCE=CATGTGCAGATACTCCAATGCT
PRIMER_LEFT=441,22
PRIMER_RIGHT=618,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.162
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.2902
PRIMER_LEFT_1_PENALTY=0.128591
PRIMER_RIGHT_1_PENALTY=0.161579
PRIMER_LEFT_1_SEQUENCE=TTTCTCCCTAGTCTCTATGCCG
PRIMER_RIGHT_1_SEQUENCE=CATGTGCAGATACTCCAATGCT
PRIMER_LEFT_1=439,22
PRIMER_RIGHT_1=618,22
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=60.162
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=11.7000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.3822
PRIMER_LEFT_2_PENALTY=0.220659
PRIMER_RIGHT_2_PENALTY=0.161579
PRIMER_LEFT_2_SEQUENCE=TTCGTTCTACTACGCCATGAAA
PRIMER_RIGHT_2_SEQUENCE=CATGTGCAGATACTCCAATGCT
PRIMER_LEFT_2=330,22
PRIMER_RIGHT_2=618,22
PRIMER_LEFT_2_TM=59.779
PRIMER_RIGHT_2_TM=60.162
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.4384
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.436048
PRIMER_LEFT_3_SEQUENCE=TCTCCCTAGTCTCTATGCCGAG
PRIMER_RIGHT_3_SEQUENCE=CTGACTAACACGTTTTCCGGTT
PRIMER_LEFT_3=441,22
PRIMER_RIGHT_3=665,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=60.436
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=0.4849
PRIMER_LEFT_4_PENALTY=0.323355
PRIMER_RIGHT_4_PENALTY=0.161579
PRIMER_LEFT_4_SEQUENCE=CTACGCCATGAAAGTTGTTGAC
PRIMER_RIGHT_4_SEQUENCE=CATGTGCAGATACTCCAATGCT
PRIMER_LEFT_4=339,22
PRIMER_RIGHT_4=618,22
PRIMER_LEFT_4_TM=59.677
PRIMER_RIGHT_4_TM=60.162
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_05460 Ps_contig_mira-and-tgicl-ass_franssen_37872
SEQUENCE=AAACATAAAACACATCAAGCTTCCTTCCTTAAACTCTGTCTTTCTTTCTCTCTCTAACCTCTTCTTCACTCACTTTACTCTCTCACTCTTCATTCATCTCCGTTCAAAACCAAACACAACCTCCACTTTTTCTCTCACTCTCTTTCTTCCTCAATTTCCACACCAAATCCAATGCCACCCATTTCCTAAAACCCAAAACCCAACACACACAACAAAAAAACAACAACAACACCATGTCATTTTACGACGAATCTTTCTTCAACACACAACTCTCAAAACGAACCTCAATCTTCGGTCTCCACCTCTGGGTCATCATCGGAATCCTCGTCGGTTCCTTCATCGTCATAACTCTCTTCCTCATCTCCATCTGTCTCACTTCACGCCGCCGCAAAAANCACCACCACCACCACCACCAATCCACCCAAATATCTCCCTTTCACAAACACCCGCGGCAAAACCACCCCAACCCCGG
TARGET=219,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3864
PRIMER_LEFT_PENALTY=0.189529
PRIMER_RIGHT_PENALTY=0.196903
PRIMER_LEFT_SEQUENCE=AAACCAAACACAACCTCCACTT
PRIMER_RIGHT_SEQUENCE=TGAGACAGATGGAGATGAGGAA
PRIMER_LEFT=106,22
PRIMER_RIGHT=374,22
PRIMER_LEFT_TM=59.810
PRIMER_RIGHT_TM=59.803
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=269
PRIMER_PAIR_PENALTY_1=0.3864
PRIMER_LEFT_1_PENALTY=0.189529
PRIMER_RIGHT_1_PENALTY=0.196903
PRIMER_LEFT_1_SEQUENCE=AAAACCAAACACAACCTCCACT
PRIMER_RIGHT_1_SEQUENCE=TGAGACAGATGGAGATGAGGAA
PRIMER_LEFT_1=105,22
PRIMER_RIGHT_1=374,22
PRIMER_LEFT_1_TM=59.810
PRIMER_RIGHT_1_TM=59.803
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=270
PRIMER_PAIR_PENALTY_2=0.3864
PRIMER_LEFT_2_PENALTY=0.189529
PRIMER_RIGHT_2_PENALTY=0.196903
PRIMER_LEFT_2_SEQUENCE=ACCAAACACAACCTCCACTTTT
PRIMER_RIGHT_2_SEQUENCE=TGAGACAGATGGAGATGAGGAA
PRIMER_LEFT_2=108,22
PRIMER_RIGHT_2=374,22
PRIMER_LEFT_2_TM=59.810
PRIMER_RIGHT_2_TM=59.803
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=0.3864
PRIMER_LEFT_3_PENALTY=0.189529
PRIMER_RIGHT_3_PENALTY=0.196903
PRIMER_LEFT_3_SEQUENCE=AACCAAACACAACCTCCACTTT
PRIMER_RIGHT_3_SEQUENCE=TGAGACAGATGGAGATGAGGAA
PRIMER_LEFT_3=107,22
PRIMER_RIGHT_3=374,22
PRIMER_LEFT_3_TM=59.810
PRIMER_RIGHT_3_TM=59.803
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.4146
PRIMER_LEFT_4_PENALTY=0.332137
PRIMER_RIGHT_4_PENALTY=0.082508
PRIMER_LEFT_4_SEQUENCE=TTTCTTCCTCAATTTCCACACC
PRIMER_RIGHT_4_SEQUENCE=AAGGGAGATATTTGGGTGGATT
PRIMER_LEFT_4=142,22
PRIMER_RIGHT_4=435,22
PRIMER_LEFT_4_TM=60.332
PRIMER_RIGHT_4_TM=59.917
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_05461 Ps_contig_mira-and-tgicl-ass_franssen_37872
SEQUENCE=AAACATAAAACACATCAAGCTTCCTTCCTTAAACTCTGTCTTTCTTTCTCTCTCTAACCTCTTCTTCACTCACTTTACTCTCTCACTCTTCATTCATCTCCGTTCAAAACCAAACACAACCTCCACTTTTTCTCTCACTCTCTTTCTTCCTCAATTTCCACACCAAATCCAATGCCACCCATTTCCTAAAACCCAAAACCCAACACACACAACAAAAAAACAACAACAACACCATGTCATTTTACGACGAATCTTTCTTCAACACACAACTCTCAAAACGAACCTCAATCTTCGGTCTCCACCTCTGGGTCATCATCGGAATCCTCGTCGGTTCCTTCATCGTCATAACTCTCTTCCTCATCTCCATCTGTCTCACTTCACGCCGCCGCAAAAANCACCACCACCACCACCACCAATCCACCCAAATATCTCCCTTTCACAAACACCCGCGGCAAAACCACCCCAACCCCGG
TARGET=396,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2918
PRIMER_LEFT_PENALTY=0.209271
PRIMER_RIGHT_PENALTY=0.082508
PRIMER_LEFT_SEQUENCE=AACACCATGTCATTTTACGACG
PRIMER_RIGHT_SEQUENCE=AAGGGAGATATTTGGGTGGATT
PRIMER_LEFT=227,22
PRIMER_RIGHT=435,22
PRIMER_LEFT_TM=59.791
PRIMER_RIGHT_TM=59.917
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=10.1000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.2918
PRIMER_LEFT_1_PENALTY=0.209271
PRIMER_RIGHT_1_PENALTY=0.082508
PRIMER_LEFT_1_SEQUENCE=AACACCATGTCATTTTACGACG
PRIMER_RIGHT_1_SEQUENCE=AAAGGGAGATATTTGGGTGGAT
PRIMER_LEFT_1=227,22
PRIMER_RIGHT_1=436,22
PRIMER_LEFT_1_TM=59.791
PRIMER_RIGHT_1_TM=59.917
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.1000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.3662
PRIMER_LEFT_2_PENALTY=0.283676
PRIMER_RIGHT_2_PENALTY=0.082508
PRIMER_LEFT_2_SEQUENCE=CGACGAATCTTTCTTCAACACA
PRIMER_RIGHT_2_SEQUENCE=AAGGGAGATATTTGGGTGGATT
PRIMER_LEFT_2=244,22
PRIMER_RIGHT_2=435,22
PRIMER_LEFT_2_TM=60.284
PRIMER_RIGHT_2_TM=59.917
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=192
PRIMER_PAIR_PENALTY_3=0.3662
PRIMER_LEFT_3_PENALTY=0.283676
PRIMER_RIGHT_3_PENALTY=0.082508
PRIMER_LEFT_3_SEQUENCE=CGACGAATCTTTCTTCAACACA
PRIMER_RIGHT_3_SEQUENCE=AAAGGGAGATATTTGGGTGGAT
PRIMER_LEFT_3=244,22
PRIMER_RIGHT_3=436,22
PRIMER_LEFT_3_TM=60.284
PRIMER_RIGHT_3_TM=59.917
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.3742
PRIMER_LEFT_4_PENALTY=0.291669
PRIMER_RIGHT_4_PENALTY=0.082508
PRIMER_LEFT_4_SEQUENCE=ACACCATGTCATTTTACGACGA
PRIMER_RIGHT_4_SEQUENCE=AAGGGAGATATTTGGGTGGATT
PRIMER_LEFT_4=228,22
PRIMER_RIGHT_4=435,22
PRIMER_LEFT_4_TM=60.292
PRIMER_RIGHT_4_TM=59.917
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=208
=
PRIMER_SEQUENCE_ID=SSR_05462 Ps_contig_mira-and-tgicl-ass_franssen_37876
SEQUENCE=GTCTTCACATTTGCATACTTTGGATCTAAACGATATTTGGTCTCTTTATCATCATCATCTTCCTGATCCATTGAAGTCTCACTCATAGTGCTGCATTTTCGTATTTCCTAATTCTCATGAATTCTTTTTTGACTTGCCCTCTGTATCTTCATTTTCATTTTTCATGGATAATAGTGAATTGTCTTGGTTATTCTTTAGCTCGGCGACACTAATTGCTTCCTCTCGAGTGTTTCTCACATCAAT
TARGET=48,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05463 Ps_contig_mira-and-tgicl-ass_franssen_37882
SEQUENCE=ACTGCTGCNCAAAGTAGCTTCCTAAACCGCTCCACCAATGCTACGAGCTTCGCTCCAAACGCTGCACAATACTCCAAYAGAAGCAACACGCCAAGCCAAAACGCGATGCAATTCCAACTACAAGCCAAGCTACTTCCAAGCACCAAATAAGCTACAGTTACAATTAAGCCGGTAAACCGCAGCTAAACGCGAGCCAGCACAAGCCAATACCAAAACCACTCTATAAAAACCTCTTCCGGATTCTTCTTCTTCTTCCTCCATCACCTAATCTTCTTCAACCTCTAACAACTGGACCAAGCCTCTCTTCAATTCCTTCAACAACCGTAACAAACTCACAAAACCGTTAACCACCATTGCAACCTCTCTTCAATCCCTAACCACCACCTTCACTCATTCCGTTTCTCACCAATACTCCCTCGCGAGTTC
TARGET=241,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05464 Ps_contig_mira-and-tgicl-ass_franssen_37883
SEQUENCE=GATAAGGGGAAGTTAGCGGTAGAAAATCGAGGGTTGGATTCTCGTGATAACGTTGAAGTTTCTAAAGAACTGATTGTGAAGGAAATTGAGTGTGGAACTGGTAGAAATACTACTGAGAGCAAATGGAAAAATGCCACTCCCACTGAGGAAAGGAATAAATCTAATGAAGTTGTTGGAACGAGTGGGACAAATTTCACTTTCTTGAAGAGTTCAGAGGACAGTGTGTTTGATTTACAATCGTGGAAGATCAATGGTCCTGCAGAGGCTTATCAAAATGACAGTGGAAGCAAGGCTAGTAATAATACTTTGAAGGCTTCGATTTCTCAGCAATCGAAAAATCAAACGAGTGAGACATTTGATGCAGCCAACAGTAATGTAAAAACTACAGAGGAAAATAGTGTGTCCGCCGAGAAAAAATCCTCGTTGCTTGGAAGTAGTAGTAGTGGTAAAGCCTCTACGGAACCAAAGTTTAACATTGCACAAAACAAAGAATCTAAGGAAATTGATCGGCAGCAACTTAAATTTAATAGTTCGTCGCTTAAAGAGAATTTGGCAGATAACGTCGTGTTGAGAGCTGACGAGACGCCAATTCATCTTCTGAAGTATGGGAAAAGTATTTTCGGTTTCCCAATTACCTAAAACGGTTACCCCCGGTTTT
TARGET=433,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3114
PRIMER_LEFT_PENALTY=0.283676
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=TCAAAATGACAGTGGAAGCAAG
PRIMER_RIGHT_SEQUENCE=ACGACGTTATCTGCCAAATTCT
PRIMER_LEFT=269,22
PRIMER_RIGHT=565,22
PRIMER_LEFT_TM=60.284
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.3783
PRIMER_LEFT_1_PENALTY=0.320870
PRIMER_RIGHT_1_PENALTY=0.057411
PRIMER_LEFT_1_SEQUENCE=ACGAGTGAGACATTTGATGCAG
PRIMER_RIGHT_1_SEQUENCE=CAGCTCTCAACACGACGTTATC
PRIMER_LEFT_1=342,22
PRIMER_RIGHT_1=576,22
PRIMER_LEFT_1_TM=60.321
PRIMER_RIGHT_1_TM=59.943
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.3783
PRIMER_LEFT_2_PENALTY=0.320870
PRIMER_RIGHT_2_PENALTY=0.057411
PRIMER_LEFT_2_SEQUENCE=ACGAGTGAGACATTTGATGCAG
PRIMER_RIGHT_2_SEQUENCE=TCAGCTCTCAACACGACGTTAT
PRIMER_LEFT_2=342,22
PRIMER_RIGHT_2=577,22
PRIMER_LEFT_2_TM=60.321
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.3783
PRIMER_LEFT_3_PENALTY=0.320870
PRIMER_RIGHT_3_PENALTY=0.057411
PRIMER_LEFT_3_SEQUENCE=ACGAGTGAGACATTTGATGCAG
PRIMER_RIGHT_3_SEQUENCE=GCTCTCAACACGACGTTATCTG
PRIMER_LEFT_3=342,22
PRIMER_RIGHT_3=574,22
PRIMER_LEFT_3_TM=60.321
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.4263
PRIMER_LEFT_4_PENALTY=0.093026
PRIMER_RIGHT_4_PENALTY=0.333229
PRIMER_LEFT_4_SEQUENCE=ACGAGTGGGACAAATTTCACTT
PRIMER_RIGHT_4_SEQUENCE=TAAACTTTGGTTCCGTAGAGGC
PRIMER_LEFT_4=177,22
PRIMER_RIGHT_4=471,22
PRIMER_LEFT_4_TM=59.907
PRIMER_RIGHT_4_TM=59.667
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_05465 Ps_contig_mira-and-tgicl-ass_franssen_37892
SEQUENCE=AACAAAAGGCAACTTCTGGTTGACCAAGTAAAGAAACAAATATCTCACTACATACAAATATCTACCAAACCTTCAACGTAACATTGGCAAACAATAGACAAATGACAAAACTTCTTTAACTGAAATTACAATTTCTCTGTACGCCAAAATTATACAATTAAAACCCTTTACTTAATATTGAAGATATATATAAAACTTTTCTTGCTATATATATAGATGTGTCTTTTGTAGTTGTAACTTGTAATCCAAAAACTATTCAGATGATACTCCATGGAAGAAGAAGAAGGCAACCCAATTGAATTTTAATTAAATCAAACCAACAAAACAAAATTCAGGAACAGCATGAAAAATAGCTACTGTTTTTCTTGGATGTAGGGTTACCATCGTTAACAAGACTAACCCTGTCGACGGATAATTCAGCTTTGTAAGAGTCTGAGTTGAGGACCTTCCTGCTAACATTGTTGTATATTTCTCTAATAACCATCTCAAAAGCTGTTTTCACATTTGTAGAATCCAAAGCAGAGGTTTCCATGAAAAACAAACCTTCTGATTCTGCAAGGCTTTTACCTTCTTCAATGCTTACATCTCTTATATTATCCAAATCGCATTTGTTTCCCACCAGCATCATTGCCACCGTTGTATCGCAATGAGTCTTGAGTTCATCGAGCCATCTACCGACGCTATCAAAGGTAGTTCTCCGGCTAATATCATAGACAACGAGAGCACCGACGGCGCCTCTGTAGTACGCGGAAGTAACAGCGCGAAACCGTTCTTGACCGGCGGTATCCCAAATCTGAGCCTTAACTTCTTTAGAATCAATTTCTAAGCTCTGAGTTTGAAATTCAACTCCGATTGTAGCTTTGGAGTGAAGATTGAACTCGTTACGAGCGTATCGTGAGAGTAGGTTAGATTTTCCGACGGCGGAGTCGCCGATAATGACGACTTTGAAGAGATACTCTTCGCCTCCTTCGTCTTCTGAACTTGACATTGTGTAACTGAAAGTGTGTGTGTGTGAGTGAATGAGCTTTTTGGAAGAGAAATTTTGAGAGTTTTTGGGTGAGGCGGTGTCTCGA
TARGET=206,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7416
PRIMER_LEFT_PENALTY=0.276218
PRIMER_RIGHT_PENALTY=0.465395
PRIMER_LEFT_SEQUENCE=AAACCTTCAACGTAACATTGGC
PRIMER_RIGHT_SEQUENCE=ATTGGGTTGCCTTCTTCTTCTT
PRIMER_LEFT=66,22
PRIMER_RIGHT=295,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=60.465
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.7416
PRIMER_LEFT_1_PENALTY=0.276218
PRIMER_RIGHT_1_PENALTY=0.465395
PRIMER_LEFT_1_SEQUENCE=AAACCTTCAACGTAACATTGGC
PRIMER_RIGHT_1_SEQUENCE=AATTGGGTTGCCTTCTTCTTCT
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=60.465
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.9076
PRIMER_LEFT_2_PENALTY=0.442180
PRIMER_RIGHT_2_PENALTY=0.465395
PRIMER_LEFT_2_SEQUENCE=CTTCAACGTAACATTGGCAAAC
PRIMER_RIGHT_2_SEQUENCE=ATTGGGTTGCCTTCTTCTTCTT
PRIMER_LEFT_2=70,22
PRIMER_RIGHT_2=295,22
PRIMER_LEFT_2_TM=59.558
PRIMER_RIGHT_2_TM=60.465
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.9076
PRIMER_LEFT_3_PENALTY=0.442180
PRIMER_RIGHT_3_PENALTY=0.465395
PRIMER_LEFT_3_SEQUENCE=CTTCAACGTAACATTGGCAAAC
PRIMER_RIGHT_3_SEQUENCE=AATTGGGTTGCCTTCTTCTTCT
PRIMER_LEFT_3=70,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=59.558
PRIMER_RIGHT_3_TM=60.465
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=1.2226
PRIMER_LEFT_4_PENALTY=0.276218
PRIMER_RIGHT_4_PENALTY=0.946348
PRIMER_LEFT_4_SEQUENCE=AAACCTTCAACGTAACATTGGC
PRIMER_RIGHT_4_SEQUENCE=GGTTGCCTTCTTCTTCTTCCAT
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=60.946
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_05466 Ps_contig_mira-and-tgicl-ass_franssen_37892
SEQUENCE=AACAAAAGGCAACTTCTGGTTGACCAAGTAAAGAAACAAATATCTCACTACATACAAATATCTACCAAACCTTCAACGTAACATTGGCAAACAATAGACAAATGACAAAACTTCTTTAACTGAAATTACAATTTCTCTGTACGCCAAAATTATACAATTAAAACCCTTTACTTAATATTGAAGATATATATAAAACTTTTCTTGCTATATATATAGATGTGTCTTTTGTAGTTGTAACTTGTAATCCAAAAACTATTCAGATGATACTCCATGGAAGAAGAAGAAGGCAACCCAATTGAATTTTAATTAAATCAAACCAACAAAACAAAATTCAGGAACAGCATGAAAAATAGCTACTGTTTTTCTTGGATGTAGGGTTACCATCGTTAACAAGACTAACCCTGTCGACGGATAATTCAGCTTTGTAAGAGTCTGAGTTGAGGACCTTCCTGCTAACATTGTTGTATATTTCTCTAATAACCATCTCAAAAGCTGTTTTCACATTTGTAGAATCCAAAGCAGAGGTTTCCATGAAAAACAAACCTTCTGATTCTGCAAGGCTTTTACCTTCTTCAATGCTTACATCTCTTATATTATCCAAATCGCATTTGTTTCCCACCAGCATCATTGCCACCGTTGTATCGCAATGAGTCTTGAGTTCATCGAGCCATCTACCGACGCTATCAAAGGTAGTTCTCCGGCTAATATCATAGACAACGAGAGCACCGACGGCGCCTCTGTAGTACGCGGAAGTAACAGCGCGAAACCGTTCTTGACCGGCGGTATCCCAAATCTGAGCCTTAACTTCTTTAGAATCAATTTCTAAGCTCTGAGTTTGAAATTCAACTCCGATTGTAGCTTTGGAGTGAAGATTGAACTCGTTACGAGCGTATCGTGAGAGTAGGTTAGATTTTCCGACGGCGGAGTCGCCGATAATGACGACTTTGAAGAGATACTCTTCGCCTCCTTCGTCTTCTGAACTTGACATTGTGTAACTGAAAGTGTGTGTGTGTGAGTGAATGAGCTTTTTGGAAGAGAAATTTTGAGAGTTTTTGGGTGAGGCGGTGTCTCGA
TARGET=274,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3751
PRIMER_LEFT_PENALTY=0.276218
PRIMER_RIGHT_PENALTY=1.098855
PRIMER_LEFT_SEQUENCE=AAACCTTCAACGTAACATTGGC
PRIMER_RIGHT_SEQUENCE=GCTATTTTTCATGCTGTTCCTG
PRIMER_LEFT=66,22
PRIMER_RIGHT=353,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=58.901
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=288
PRIMER_PAIR_PENALTY_1=1.5410
PRIMER_LEFT_1_PENALTY=0.442180
PRIMER_RIGHT_1_PENALTY=1.098855
PRIMER_LEFT_1_SEQUENCE=CTTCAACGTAACATTGGCAAAC
PRIMER_RIGHT_1_SEQUENCE=GCTATTTTTCATGCTGTTCCTG
PRIMER_LEFT_1=70,22
PRIMER_RIGHT_1=353,22
PRIMER_LEFT_1_TM=59.558
PRIMER_RIGHT_1_TM=58.901
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=2.5124
PRIMER_LEFT_2_PENALTY=1.413531
PRIMER_RIGHT_2_PENALTY=1.098855
PRIMER_LEFT_2_SEQUENCE=ACCTTCAACGTAACATTGGCA
PRIMER_RIGHT_2_SEQUENCE=GCTATTTTTCATGCTGTTCCTG
PRIMER_LEFT_2=68,21
PRIMER_RIGHT_2=353,22
PRIMER_LEFT_2_TM=60.414
PRIMER_RIGHT_2_TM=58.901
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=2.7368
PRIMER_LEFT_3_PENALTY=1.637916
PRIMER_RIGHT_3_PENALTY=1.098855
PRIMER_LEFT_3_SEQUENCE=AACCTTCAACGTAACATTGGCA
PRIMER_RIGHT_3_SEQUENCE=GCTATTTTTCATGCTGTTCCTG
PRIMER_LEFT_3=67,22
PRIMER_RIGHT_3=353,22
PRIMER_LEFT_3_TM=61.638
PRIMER_RIGHT_3_TM=58.901
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=3.0887
PRIMER_LEFT_4_PENALTY=1.989880
PRIMER_RIGHT_4_PENALTY=1.098855
PRIMER_LEFT_4_SEQUENCE=AACCTTCAACGTAACATTGGC
PRIMER_RIGHT_4_SEQUENCE=GCTATTTTTCATGCTGTTCCTG
PRIMER_LEFT_4=67,21
PRIMER_RIGHT_4=353,22
PRIMER_LEFT_4_TM=59.010
PRIMER_RIGHT_4_TM=58.901
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_05467 Ps_contig_mira-and-tgicl-ass_franssen_37892
SEQUENCE=AACAAAAGGCAACTTCTGGTTGACCAAGTAAAGAAACAAATATCTCACTACATACAAATATCTACCAAACCTTCAACGTAACATTGGCAAACAATAGACAAATGACAAAACTTCTTTAACTGAAATTACAATTTCTCTGTACGCCAAAATTATACAATTAAAACCCTTTACTTAATATTGAAGATATATATAAAACTTTTCTTGCTATATATATAGATGTGTCTTTTGTAGTTGTAACTTGTAATCCAAAAACTATTCAGATGATACTCCATGGAAGAAGAAGAAGGCAACCCAATTGAATTTTAATTAAATCAAACCAACAAAACAAAATTCAGGAACAGCATGAAAAATAGCTACTGTTTTTCTTGGATGTAGGGTTACCATCGTTAACAAGACTAACCCTGTCGACGGATAATTCAGCTTTGTAAGAGTCTGAGTTGAGGACCTTCCTGCTAACATTGTTGTATATTTCTCTAATAACCATCTCAAAAGCTGTTTTCACATTTGTAGAATCCAAAGCAGAGGTTTCCATGAAAAACAAACCTTCTGATTCTGCAAGGCTTTTACCTTCTTCAATGCTTACATCTCTTATATTATCCAAATCGCATTTGTTTCCCACCAGCATCATTGCCACCGTTGTATCGCAATGAGTCTTGAGTTCATCGAGCCATCTACCGACGCTATCAAAGGTAGTTCTCCGGCTAATATCATAGACAACGAGAGCACCGACGGCGCCTCTGTAGTACGCGGAAGTAACAGCGCGAAACCGTTCTTGACCGGCGGTATCCCAAATCTGAGCCTTAACTTCTTTAGAATCAATTTCTAAGCTCTGAGTTTGAAATTCAACTCCGATTGTAGCTTTGGAGTGAAGATTGAACTCGTTACGAGCGTATCGTGAGAGTAGGTTAGATTTTCCGACGGCGGAGTCGCCGATAATGACGACTTTGAAGAGATACTCTTCGCCTCCTTCGTCTTCTGAACTTGACATTGTGTAACTGAAAGTGTGTGTGTGTGAGTGAATGAGCTTTTTGGAAGAGAAATTTTGAGAGTTTTTGGGTGAGGCGGTGTCTCGA
TARGET=1002,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8929
PRIMER_LEFT_PENALTY=0.406964
PRIMER_RIGHT_PENALTY=0.485895
PRIMER_LEFT_SEQUENCE=CCTCCTTCGTCTTCTGAACTTG
PRIMER_RIGHT_SEQUENCE=CCTCACCCAAAAACTCTCAAAA
PRIMER_LEFT=962,22
PRIMER_RIGHT=1061,22
PRIMER_LEFT_TM=60.407
PRIMER_RIGHT_TM=60.486
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=1.0227
PRIMER_LEFT_1_PENALTY=0.536763
PRIMER_RIGHT_1_PENALTY=0.485895
PRIMER_LEFT_1_SEQUENCE=GGTATCCCAAATCTGAGCCTTA
PRIMER_RIGHT_1_SEQUENCE=CCTCACCCAAAAACTCTCAAAA
PRIMER_LEFT_1=781,22
PRIMER_RIGHT_1=1061,22
PRIMER_LEFT_1_TM=59.463
PRIMER_RIGHT_1_TM=60.486
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=1.0840
PRIMER_LEFT_2_PENALTY=0.598109
PRIMER_RIGHT_2_PENALTY=0.485895
PRIMER_LEFT_2_SEQUENCE=TTCAACTCCGATTGTAGCTTTG
PRIMER_RIGHT_2_SEQUENCE=CCTCACCCAAAAACTCTCAAAA
PRIMER_LEFT_2=841,22
PRIMER_RIGHT_2=1061,22
PRIMER_LEFT_2_TM=59.402
PRIMER_RIGHT_2_TM=60.486
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=221
PRIMER_PAIR_PENALTY_3=1.1311
PRIMER_LEFT_3_PENALTY=0.645218
PRIMER_RIGHT_3_PENALTY=0.485895
PRIMER_LEFT_3_SEQUENCE=TTTGGAGTGAAGATTGAACTCG
PRIMER_RIGHT_3_SEQUENCE=CCTCACCCAAAAACTCTCAAAA
PRIMER_LEFT_3=859,22
PRIMER_RIGHT_3=1061,22
PRIMER_LEFT_3_TM=59.355
PRIMER_RIGHT_3_TM=60.486
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=1.2220
PRIMER_LEFT_4_PENALTY=0.736104
PRIMER_RIGHT_4_PENALTY=0.485895
PRIMER_LEFT_4_SEQUENCE=CCCAAATCTGAGCCTTAACTTC
PRIMER_RIGHT_4_SEQUENCE=CCTCACCCAAAAACTCTCAAAA
PRIMER_LEFT_4=786,22
PRIMER_RIGHT_4=1061,22
PRIMER_LEFT_4_TM=59.264
PRIMER_RIGHT_4_TM=60.486
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_05468 Ps_contig_mira-and-tgicl-ass_franssen_37908
SEQUENCE=CCAACCCCAATTTGTATTTACAGAACAAATTGATCTGAAATAATTGAAAATAAAATAGCTATGCACTACGGAGTAATATAAGAAATAATAAGAATAAACTTGGATCTATTAATCTATTAATCAGTAATGAGGAATTTCAATCTTGAAAAGAAAGGGCTTCTTTGCTGCAGGAAGAGGGCTGGAAACCTCGTCATCCACGAAACACTTCTTTGAAAAGTCCGGAAAAGCCGGGAGGTTAAGATCAAAGTCACGGTGTTGGCTGTGAGAGGATCCAACTCCTTCCGAGGCCGTTACGGCGCTACTTCCGGCTCCGCCACCGGCACCGCCTTCGTAGTGGCAACGCTTGTGTCCTCCCAAAGCCTGTCCGGTAGGGAAGGATTTGTGACATATGGAACAATCGTGAGTCTTGATCTTATTGGATGCAGAGCTAGTAGTCACGGCGGATGAGGTGGTGGTGGTCTGATTCTGATCTTCGGCAGTGGACATAAGCGCGAGTTTCCGGTGGCTGGCTTTGTGTCCGCCGAGTGCTTGGTAAGATGAGAAAGCTTTGTTGCAAACGGAGCATTTGTGACTGAGTTTAACGGTTGTTAGCGGCGCCGATTCAGTGTCAGATAAAATCTGTTTCGCGGTGGAGTTGTTGCTGTTGTTGCTGTTGTTGTTGCCGCTGCGAGCGAGCATGATGAGACAGAAAGCGAGATACTCTTCTTCACTGCATGAAGATTGATCCATACGAGAACGCTTTGAACGTTTACCTTTCGTCCATGGTGTATCGAGGTAGCTGAGATTCGGTTCCTCGAAGGGAGTGAAAGGAGTGGCGGTGGTGGGTGAGTTAAGCGCTTCCGTAGCCATAGAGTTGAGAGAAAAATGAGAGGATTGTGTTGTTTGATTTTTTTTTGGAGTGTGATCNYCCNAGTACTCTGCGTGTATA
TARGET=448,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05469 Ps_contig_mira-and-tgicl-ass_franssen_37910
SEQUENCE=ACTCTCTCTCTCTTTATGTAAACTTTCCACTCGTAAACACCAAGACAATTACAAAATCTTTGAGCTACACCTCCTTCTACCTCTGTTTCCTAAACAATGAAACCCTCCACGCTTCATCTTTTTGTCTTGTTCTTTCTTCTAAGCTATGTTCAAGGTCGTTTTGTAGTGGAGAAAAACAGCATAACTGTTCTGTCACCACATAAATTACGAGGTAAAAAAGATGGTGCCATAGGAAACTTTGGAATTCCAAATTATGGAGGCTATATTCTTGGATCACTTGTTTATCCTGAATAAAGGCTCTCATGGTTGTCAAGTCTC
TARGET=2,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05470 Ps_contig_mira-and-tgicl-ass_franssen_37914
SEQUENCE=CTTCTTCAACAAAGTAGCGAGTTGGACTAAACCTACCTCTCTCTCTAAGAAGAAGAGAGGATTTAGCATCATCAAGACCAGGACCAACGTCCAGAATCGCTTCTAGATAACTATTCAACCAATCATTTCCTGCCATTTTTCACTGCAACTACTGCTTTAAGATAATTGCGCTTATAAAGATGAAGAAGATGAGTGAAGAGAATTTAATTAGAAAAAATAAAAGAGAAAATAATAAAAGAGAATATTCGTTAGAACTCTGGAAATGGACTATGGAGTGA
TARGET=37,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2532
PRIMER_LEFT_PENALTY=0.080323
PRIMER_RIGHT_PENALTY=0.172839
PRIMER_LEFT_SEQUENCE=TTCAACAAAGTAGCGAGTTGGA
PRIMER_RIGHT_SEQUENCE=GTAGTTGCAGTGAAAAATGGCA
PRIMER_LEFT=4,22
PRIMER_RIGHT=151,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=60.173
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=0.3780
PRIMER_LEFT_1_PENALTY=0.080323
PRIMER_RIGHT_1_PENALTY=0.297699
PRIMER_LEFT_1_SEQUENCE=TTCAACAAAGTAGCGAGTTGGA
PRIMER_RIGHT_1_SEQUENCE=TAGTTGCAGTGAAAAATGGCAG
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=150,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=60.298
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.7484
PRIMER_LEFT_2_PENALTY=0.575571
PRIMER_RIGHT_2_PENALTY=0.172839
PRIMER_LEFT_2_SEQUENCE=TCAACAAAGTAGCGAGTTGGAC
PRIMER_RIGHT_2_SEQUENCE=GTAGTTGCAGTGAAAAATGGCA
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=151,22
PRIMER_LEFT_2_TM=59.424
PRIMER_RIGHT_2_TM=60.173
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=0.8733
PRIMER_LEFT_3_PENALTY=0.575571
PRIMER_RIGHT_3_PENALTY=0.297699
PRIMER_LEFT_3_SEQUENCE=TCAACAAAGTAGCGAGTTGGAC
PRIMER_RIGHT_3_SEQUENCE=TAGTTGCAGTGAAAAATGGCAG
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=150,22
PRIMER_LEFT_3_TM=59.424
PRIMER_RIGHT_3_TM=60.298
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=1.0281
PRIMER_LEFT_4_PENALTY=0.080323
PRIMER_RIGHT_4_PENALTY=0.947776
PRIMER_LEFT_4_SEQUENCE=TTCAACAAAGTAGCGAGTTGGA
PRIMER_RIGHT_4_SEQUENCE=TCACTCCATAGTCCATTTCCAG
PRIMER_LEFT_4=4,22
PRIMER_RIGHT_4=277,22
PRIMER_LEFT_4_TM=59.920
PRIMER_RIGHT_4_TM=59.052
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_05471 Ps_contig_mira-and-tgicl-ass_franssen_37916
SEQUENCE=AAAATAAAATATAAAAAAAAGANAATAACAATAAGAGCAGAGAAAGAGAAAGAGAAGAAAGAAATATTCATAAAGTTTCTTCTTTCAACCTAACAAAACTTCAGTGCTGTTTATCCTTCAATTCTTCTTCTTCTTTGTTTTAACAGAAAAATGGAGTTTGCGGTGGCAAAAGCTTTGAAACCGAGTTTGAGGAGGGAGTTTGTTTTTCAACAAACGCTTGGTGAAGAACTGTTTAGCTTTAACTCGAACACGGTGGTTGCCGGTGAAGATTTCTCGGTGGACGACCTCCTTGACTTCTCCAACGGCGATGAACAACACCACGAGGGAGATGAAGAAGAAAAAGATAGTTTATCTCTTTCCTCTCACTCGCTCTCGGAAGATAGCAACTCCAATTCCACCGACGCTTCCTATGATTCCATTTTCACAAATGAATTACTCGTTCCGGATGATGACGTGGCAGGCCTTGAATGGGTTTCTCAYTTTGTAGATGATTCTTTACCAGARNNTATTTAGTGGTTGTAGTTGTAGTGGTAGTCAGGTAGGCAGCAGGTAAGGGATATTATTAGGAAGGTATGGTAGTTGTTTTTATTTTTATTCCTATTTTCTTAGTCTTTTGTTTCTTTTAATTAATCTAATACTAAGTTAAAATTAAAAAAAGTTTT
TARGET=122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05472 Ps_contig_mira-and-tgicl-ass_franssen_37923
SEQUENCE=TAGTTTTCGCNTAAAAAACATGTCGATGCGTCAAAACATAGCCCCAAACATTGTTCCAAAGATACACCAAGTGAGATGACGGTTCACTAATTAACAAACATAAAGGTACTTTTGAAATATACGGTTTTCTACTGGTAAACTCATCACGTTCTTATTATTTATAATTTGGTGCCTTATGCAACGGCTTTTGTTGTCCTAATTCAAATCAAACCGTATCATACATGTTCTATAGATGCATAAGCTGGCGAGCCAGCAACTGGTTTGTGTAGTAAAAATCAAGTACACTTGGGAAGAGGAGAACCACACAAGCACTTATTATGAAAATATTCATAAATATCAAAACTTTGCAATTTTCCACCCACCGGAATCTGACCGCACAACCTATTGTAACTCACATTCAGGTACTGCAGATTCAGTGCAGTCAATCCCTGAGGAAGCTTCCCATACACCTTATTATGATTAAGATCCAACGACGTCAAGCTCGTGGAAAACTCCACCTTCGACAGATCGAACGATAGGTTGTTTCTCGATAGGTCCACAATTTGGAAAGACTTGTTCAGCCCGAATATCACAGACGCATCTCCTTCAAGCTTGTTCCTCGACAAGATAAGCGAGGTGGTGGAGTTGAGGTTCACAAACGAGGGTGGAATGGGACCAGTGAGGTTGTTATGGGAGAGGTCGATACCAGCTATGTTGGACTTAAAAGAACCGAACGAGGACGGGATCGAACCGGTTAGATGGTTACGGTCTAACTGGAGTGAGAAGAGGTTGGGAAGTTGTGAGAGTGAACCGGGGATTGAACCGGTGAGGTTGTTGAAAGAGAGATCCAAAAACGCGAGGTTTGTGAGTTGGGCTAGGAAGGAAGGAACCGGGCCTGACAAGTTTGTGTTACTGATACGGAGGTTCTTGAGGTTGGTGAGTTTAGCAATAGCGGGCTGGAGCGGGCCTGTGAGGTTTGGAAGTTTGTGAAACTCGAGAGTCTCGAGGTAGGGAAGCAGGCCCACTGAGGGAGGGATTTGGGCTGAGAAATTGGTGTATACAGGAGAATCAAAAATAGTGAGGGAGATGATGCGGTGAGTTTTGAGGTCGCACTCTATGGTGTACCAGTCGCAACAATCTGTGTCTGGGTTCCATGATGCTAGAAGGTAAGGGTTGTTGAGGTCTTTCTTGATTTGGAGTAGCACTTTCTTATCTTGTGGGTTGCATAGCTCTGAAAATGCACTGGATAGCAGTATCAGAAGCAAAGTTAAGCGTAGCACAGTGGTTGTTGTCATTTTTAATGTGATTCCCGCGACTCTGCGTTGA
TARGET=856,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1189
PRIMER_LEFT_PENALTY=0.116582
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=GGAGAGGTCGATACCAGCTATG
PRIMER_RIGHT_SEQUENCE=CAACCTCAAGAACCTCCGTATC
PRIMER_LEFT=671,22
PRIMER_RIGHT=914,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=5.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.1189
PRIMER_LEFT_1_PENALTY=0.116582
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=GGAGAGGTCGATACCAGCTATG
PRIMER_RIGHT_1_SEQUENCE=AACCTCAAGAACCTCCGTATCA
PRIMER_LEFT_1=671,22
PRIMER_RIGHT_1=913,22
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.1481
PRIMER_LEFT_2_PENALTY=0.145769
PRIMER_RIGHT_2_PENALTY=0.002310
PRIMER_LEFT_2_SEQUENCE=GAGAAGAGGTTGGGAAGTTGTG
PRIMER_RIGHT_2_SEQUENCE=AACCTCAAGAACCTCCGTATCA
PRIMER_LEFT_2=759,22
PRIMER_RIGHT_2=913,22
PRIMER_LEFT_2_TM=60.146
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.1481
PRIMER_LEFT_3_PENALTY=0.145769
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=AAGAGGTTGGGAAGTTGTGAGA
PRIMER_RIGHT_3_SEQUENCE=AACCTCAAGAACCTCCGTATCA
PRIMER_LEFT_3=762,22
PRIMER_RIGHT_3=913,22
PRIMER_LEFT_3_TM=60.146
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=0.1481
PRIMER_LEFT_4_PENALTY=0.145769
PRIMER_RIGHT_4_PENALTY=0.002310
PRIMER_LEFT_4_SEQUENCE=AGAAGAGGTTGGGAAGTTGTGA
PRIMER_RIGHT_4_SEQUENCE=AACCTCAAGAACCTCCGTATCA
PRIMER_LEFT_4=760,22
PRIMER_RIGHT_4=913,22
PRIMER_LEFT_4_TM=60.146
PRIMER_RIGHT_4_TM=59.998
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_05473 Ps_contig_mira-and-tgicl-ass_franssen_37962
SEQUENCE=TTCTTAGAGTAAATAAGCAGATAAGAATTTTTTAATAGCTTGTGATAGGCCCTCGACAAGGCCTGTAGAGGAAAACATTATCCCCATGANNNAGAAAACAAAGAAATAAATGTTCCTTACATGTAATCTGTGTCCAAAATCCCTTTAGCTCTTCTAACAATCAAGTACTTTCTACAGCCTATTTGTACTATTCCACCACCTGATAATATAATTACAGCTTACAATAACATATAAAGAAATAAACTGACCCAAAACTCGGGCGGGGGAAGTCTGAAACAAAGATATGTTTAAAAATTATATATATAGAAAAAAAATACAACTTTGTTGATCACTATAATGTCACTTATTCATCCGTCTTTTCAAGATTAGTGTCTTCCTGACCTGTCTGAGTAGAGTTTTTTTCAATATTAGTGTCTTCCTGCCCTGTCGGAGAAGTAGAGCTTTTTTCATGATTAGTGTCTTCCTGACCTGTCTCAGAAGTAGAGATATTGTTCTCCTCATCAGTTTTGATGGCTTCAGATTCAGTATTGATGACTTCAGATTCTTTGGCATCAGATGTTTCACCATTATCCATTTTTGTCACTTCACCGTTCTCAGCTATGCCACTGGCTTCCCTATTATTTACTGGTTTTGGGAGTTCTCTTGCAGAGGAAGCCTCTACTAGGCGGCGGATTCCATCATAAACGTTCTTTGCATCCATGATAAGAGTTGCTCCACCGGTATAGCAACGCCCAAAACCATGTAGCACAATGAGGATAAGCAACCTCAATAAATGCCCGGCAAAGTGAGAGGAAAAGCCCTGATTCGTTAGCCCTAAGAACCCGTGTTGTGTTGTATAGCAACAATGAATCAGAAACAGCCTGTAACCCCTTGATCAATTCTTGGGCAAGGACATGTTTTAAACTTAG
TARGET=296,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6144
PRIMER_LEFT_PENALTY=0.588779
PRIMER_RIGHT_PENALTY=0.025669
PRIMER_LEFT_SEQUENCE=AATAAACTGACCCAAAACTCGG
PRIMER_RIGHT_SEQUENCE=TCTGAGACAGGTCAGGAAGACA
PRIMER_LEFT=237,22
PRIMER_RIGHT=477,22
PRIMER_LEFT_TM=59.411
PRIMER_RIGHT_TM=60.026
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=0.7199
PRIMER_LEFT_1_PENALTY=0.694262
PRIMER_RIGHT_1_PENALTY=0.025669
PRIMER_LEFT_1_SEQUENCE=GGGGGAAGTCTGAAACAAAGAT
PRIMER_RIGHT_1_SEQUENCE=TCTGAGACAGGTCAGGAAGACA
PRIMER_LEFT_1=261,22
PRIMER_RIGHT_1=477,22
PRIMER_LEFT_1_TM=60.694
PRIMER_RIGHT_1_TM=60.026
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.9454
PRIMER_LEFT_2_PENALTY=0.588779
PRIMER_RIGHT_2_PENALTY=0.356592
PRIMER_LEFT_2_SEQUENCE=AATAAACTGACCCAAAACTCGG
PRIMER_RIGHT_2_SEQUENCE=GACAGGGCAGGAAGACACTAAT
PRIMER_LEFT_2=237,22
PRIMER_RIGHT_2=427,22
PRIMER_LEFT_2_TM=59.411
PRIMER_RIGHT_2_TM=59.643
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=1.0378
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=1.037017
PRIMER_LEFT_3_SEQUENCE=CTGTGTCCAAAATCCCTTTAGC
PRIMER_RIGHT_3_SEQUENCE=CTCAGACAGGTCAGGAAGACACT
PRIMER_LEFT_3=127,22
PRIMER_RIGHT_3=389,23
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=59.963
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.174
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=1.0509
PRIMER_LEFT_4_PENALTY=0.694262
PRIMER_RIGHT_4_PENALTY=0.356592
PRIMER_LEFT_4_SEQUENCE=GGGGGAAGTCTGAAACAAAGAT
PRIMER_RIGHT_4_SEQUENCE=GACAGGGCAGGAAGACACTAAT
PRIMER_LEFT_4=261,22
PRIMER_RIGHT_4=427,22
PRIMER_LEFT_4_TM=60.694
PRIMER_RIGHT_4_TM=59.643
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_05474 Ps_contig_mira-and-tgicl-ass_franssen_37976
SEQUENCE=CCTTAACCCAAAAACCTAAAACCTATTTTTTTCTATCTGTTACAATCACAAAGACAAAAAACAGAGACAAAAAAAAACAATGGCTGTTTCATCTTCTTCTTCTCTTTTGCTCCTTTTTGTTCTCTTTGCTTTTGCTGCTGCAGCACCTAAGGATTACATACTTGGTGGCAAACCTGATGCGTGGAAGGTTCCGTCTTCTGAATCGGACTCTCTCAACAAATGGGCTGAAAGTGTTCGCTTCCACATTGCAGATCATCTTCTTTTTACCTATGAAGCTGGGAAAGACTCTGTTTTGCAAGTGAGCAAAGAGGATTACGTGAGCTGCAACATCTCAAACCCGATCAAAAAGTACAATGATGGGAAAACAAAGGTGAGATTTGATCGTTCAGGTCCATACTACTACATCAGTGGAGAAAAGGGTCACTGTGAAAAGGGTGAGAAAGTGACTGTGGTGGTTATAAGCCAAAGAAGTCCAAGTGTCGCTCCTGTTTCACCTGCTTCTGCGCCTTCACCAGGTGGCGGTGTTGAAGGTCCAGCCGTTGCTCCAAGTCCAACTAGCAGTGCAGATGTTTTGCAAGGTGGTGGAGTTTTGATGGCTATGGGAGTGGTGGCTTCTATGTGGGTTTTCTGATTTGAATTGCCATCTTTTTAGTTAGAATATTTCTATTATTATTATTATTATTATAAATATTGGGATTGGTTTAAAGTGTTTTAGAGACAGTCACAGTGTGGTGATTGCTAGTGCTATTATTTTGTTGGGACCATATTATTCAATTTTTAATAGTATGAATTATAATTTATATGGTTTGGCTTTC
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05475 Ps_contig_mira-and-tgicl-ass_franssen_37976
SEQUENCE=CCTTAACCCAAAAACCTAAAACCTATTTTTTTCTATCTGTTACAATCACAAAGACAAAAAACAGAGACAAAAAAAAACAATGGCTGTTTCATCTTCTTCTTCTCTTTTGCTCCTTTTTGTTCTCTTTGCTTTTGCTGCTGCAGCACCTAAGGATTACATACTTGGTGGCAAACCTGATGCGTGGAAGGTTCCGTCTTCTGAATCGGACTCTCTCAACAAATGGGCTGAAAGTGTTCGCTTCCACATTGCAGATCATCTTCTTTTTACCTATGAAGCTGGGAAAGACTCTGTTTTGCAAGTGAGCAAAGAGGATTACGTGAGCTGCAACATCTCAAACCCGATCAAAAAGTACAATGATGGGAAAACAAAGGTGAGATTTGATCGTTCAGGTCCATACTACTACATCAGTGGAGAAAAGGGTCACTGTGAAAAGGGTGAGAAAGTGACTGTGGTGGTTATAAGCCAAAGAAGTCCAAGTGTCGCTCCTGTTTCACCTGCTTCTGCGCCTTCACCAGGTGGCGGTGTTGAAGGTCCAGCCGTTGCTCCAAGTCCAACTAGCAGTGCAGATGTTTTGCAAGGTGGTGGAGTTTTGATGGCTATGGGAGTGGTGGCTTCTATGTGGGTTTTCTGATTTGAATTGCCATCTTTTTAGTTAGAATATTTCTATTATTATTATTATTATTATAAATATTGGGATTGGTTTAAAGTGTTTTAGAGACAGTCACAGTGTGGTGATTGCTAGTGCTATTATTTTGTTGGGACCATATTATTCAATTTTTAATAGTATGAATTATAATTTATATGGTTTGGCTTTC
TARGET=665,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5828
PRIMER_LEFT_PENALTY=0.205226
PRIMER_RIGHT_PENALTY=0.377552
PRIMER_LEFT_SEQUENCE=AAGCCAAAGAAGTCCAAGTGTC
PRIMER_RIGHT_SEQUENCE=AGCACTAGCAATCACCACACTG
PRIMER_LEFT=459,22
PRIMER_RIGHT=745,22
PRIMER_LEFT_TM=59.795
PRIMER_RIGHT_TM=60.378
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.7501
PRIMER_LEFT_1_PENALTY=0.372557
PRIMER_RIGHT_1_PENALTY=0.377552
PRIMER_LEFT_1_SEQUENCE=AGTTTTGATGGCTATGGGAGTG
PRIMER_RIGHT_1_SEQUENCE=AGCACTAGCAATCACCACACTG
PRIMER_LEFT_1=585,22
PRIMER_RIGHT_1=745,22
PRIMER_LEFT_1_TM=60.373
PRIMER_RIGHT_1_TM=60.378
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=161
PRIMER_PAIR_PENALTY_2=1.0021
PRIMER_LEFT_2_PENALTY=0.624506
PRIMER_RIGHT_2_PENALTY=0.377552
PRIMER_LEFT_2_SEQUENCE=CAAGTCCAACTAGCAGTGCAGA
PRIMER_RIGHT_2_SEQUENCE=AGCACTAGCAATCACCACACTG
PRIMER_LEFT_2=545,22
PRIMER_RIGHT_2=745,22
PRIMER_LEFT_2_TM=60.625
PRIMER_RIGHT_2_TM=60.378
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=1.4133
PRIMER_LEFT_3_PENALTY=1.035773
PRIMER_RIGHT_3_PENALTY=0.377552
PRIMER_LEFT_3_SEQUENCE=CTCCTGTTTCACCTGCTTCTG
PRIMER_RIGHT_3_SEQUENCE=AGCACTAGCAATCACCACACTG
PRIMER_LEFT_3=482,21
PRIMER_RIGHT_3=745,22
PRIMER_LEFT_3_TM=60.036
PRIMER_RIGHT_3_TM=60.378
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=1.5655
PRIMER_LEFT_4_PENALTY=1.187985
PRIMER_RIGHT_4_PENALTY=0.377552
PRIMER_LEFT_4_SEQUENCE=TAAGCCAAAGAAGTCCAAGTGTC
PRIMER_RIGHT_4_SEQUENCE=AGCACTAGCAATCACCACACTG
PRIMER_LEFT_4=458,23
PRIMER_RIGHT_4=745,22
PRIMER_LEFT_4_TM=59.812
PRIMER_RIGHT_4_TM=60.378
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_05476 Ps_contig_mira-and-tgicl-ass_franssen_37978
SEQUENCE=GTCGGGGGGTGTCCCCGCGTTATCTATTTATTTCCCGGACACACCCTTCTTCTTCTTTTCGTTCCTTCTTTTCTCCTATTGCAAAACTCTCCTTACCTCCAACTATCAATGGCGACAATTTCTTTACCAAATGGTTTGAAAACTCTGTTCACTGTTCTCGGTGCTCTCATGCTTGCTCTCCTTCTTTACACACTCATTACCGACGGTTTCCCCTTCCGCAAAGAACTTCTCACTCCGTGGATGGCAGCAACTTTGGTTGATTTCTATATCAACGTTGCAATTTTGAGCGCCTGGGTTGTTTACAAGGAAGCAAATTGGGTCAGCTCAATTATATGGATAATTTTACTTATCTCCCTTGGAAGCATTGCTACATCTGCCTATATTGTTGTGCAATTTTTGAAGCTGTCATCTCAAGAATCTTCACAGGACCCTATGACCTATGTTCTTTTACGACATCCACACAAGAGTGGCATAGAACCAAAAACAAAGTGTTCTGTTGTTGCAATTCTAAGAATCCTTTTCAGCGTTTTGGGCGTTGTCATGCTTGGAACCTTGGTATACACTCTTGTCACTGATGGTTCTCCTTTCCGTACGGAGCTATTGACTCCGTGGATGCAAGCAACACTAGTTGACTTCTACATCAATGTTGTGGCCCTAGCAGTCTGGGTTGCCTACAAAGAATCTAATTGGATTTATGCGGTTTTATGGGTGATTCTATTGATATGTTTTGGCAGCATCACTTCATGCATCTACGTTGTATGGCAGTTGTTCCAAATATCTTATCAGGATCCTGCTTACCTTATTTTAGTTCATCATAATGACAGAAACGGCAATGGTTATTGATGGTAGGGCAGAAAACAAATATAAGGAGATTTCTGCTGATGCAACATAGCAATGGTGTTAATCAAACAAGGTGTGGTATACTGGAAGTAAAAATGACCCCAGAAGACAGTAAATTAACATACTCTGATGAAATTGATGCCTGGAGATAGTAT
TARGET=46,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1956
PRIMER_LEFT_PENALTY=0.141625
PRIMER_RIGHT_PENALTY=0.054021
PRIMER_LEFT_SEQUENCE=CGCGTTATCTATTTATTTCCCG
PRIMER_RIGHT_SEQUENCE=AGTGAGAAGTTCTTTGCGGAAG
PRIMER_LEFT=15,22
PRIMER_RIGHT=233,22
PRIMER_LEFT_TM=59.858
PRIMER_RIGHT_TM=60.054
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.2219
PRIMER_LEFT_1_PENALTY=0.141625
PRIMER_RIGHT_1_PENALTY=0.080323
PRIMER_LEFT_1_SEQUENCE=CGCGTTATCTATTTATTTCCCG
PRIMER_RIGHT_1_SEQUENCE=CCGTCGGTAATGAGTGTGTAAA
PRIMER_LEFT_1=15,22
PRIMER_RIGHT_1=205,22
PRIMER_LEFT_1_TM=59.858
PRIMER_RIGHT_1_TM=59.920
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=11.4000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.2739
PRIMER_LEFT_2_PENALTY=0.219844
PRIMER_RIGHT_2_PENALTY=0.054021
PRIMER_LEFT_2_SEQUENCE=GTGTCCCCGCGTTATCTATTTA
PRIMER_RIGHT_2_SEQUENCE=AGTGAGAAGTTCTTTGCGGAAG
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=233,22
PRIMER_LEFT_2_TM=60.220
PRIMER_RIGHT_2_TM=60.054
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=0.2927
PRIMER_LEFT_3_PENALTY=0.141625
PRIMER_RIGHT_3_PENALTY=0.151106
PRIMER_LEFT_3_SEQUENCE=CGCGTTATCTATTTATTTCCCG
PRIMER_RIGHT_3_SEQUENCE=AAAGAAGGAGAGCAAGCATGAG
PRIMER_LEFT_3=15,22
PRIMER_RIGHT_3=186,22
PRIMER_LEFT_3_TM=59.858
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.3209
PRIMER_LEFT_4_PENALTY=0.266928
PRIMER_RIGHT_4_PENALTY=0.054021
PRIMER_LEFT_4_SEQUENCE=TGTCCCCGCGTTATCTATTTAT
PRIMER_RIGHT_4_SEQUENCE=AGTGAGAAGTTCTTTGCGGAAG
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=59.733
PRIMER_RIGHT_4_TM=60.054
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_05477 Ps_contig_mira-and-tgicl-ass_franssen_37979
SEQUENCE=TCAGTCGTGGAGATGGTTCTCACCATCATAACTACGGGAACAGGCGTGATCAAGACTGGAATAGTCGTCGAAATTTCAATGGTAGAGACATGCATGTGCCACCAAGAGTTTCTCCAAGAATAATAAGGCCATCTCTGCCTCCTAATTCTGGACAATTTATTCATCCACAATCATTGCGGCCTTTTGGTGGCCACATGGGATTCCATGAACTAGCACCTCCAGTGGTATTTGTCGCCGCTCCACCTCCACCTCCACCCCTGGATTCACTAAGAGGTGTTCCCTATGTGCCTCCTATGGCACATCCTGCTCTTTATTATGCCGGGCCAGACCCTCAGCTGCATAGTATGATAGTTAGTCAGATTGAGTACTATTTTAGTAATGAAAATTTAGTTAGGGATACATTCTTGCGGCAGAAAATGGATGACCAGGGCTGGGTTCCAATAAAACTAATTGCAGGCTTCAAAAAAGTTATGCTTTTGACAGACAATATCCAGCTTATAATGGATGCAGTTCGAACTTCATCTGTTGTTGAAGTGCAGGGAGACAAAATAAGGAGGAAAATTGATTGGATGAAATGGATCATGCCTCCTCCTGTTCRGTTTCATAATGTTGAGACTCCAGGTGTGCTGACTCATGACACGCTCTCAGAAGAAGTGCACAACATTTCTCAGGAGACCACGATCTATGCTGGTGCTGGAGGACTAGTTCTACCAGATAAGATAAGTACTGGTCAAGTTGGTCATCATGACTCAGATGACTCTATTTCAACGAGAAATTAAAGTTACAAATGTGCTTCAACACATTGTTTTTTTTGGAGAGCCCGTAAAAGTTATGGACACATGAAGCAGGTATCTAAAAACTATCTCACTTTTTTGCACGTGGCACAATGGAGAGTAATTTCTCACTGAGACATATTTAGGATAACATGATTGAGGAATGGGAAGAAAATTCCTATTGCAAAACAGAAAACAGGACTAAATGGCAAAGTTGAACCCCTCTCCCTCTAAAAATGAACAGCCATTTCATTTTTTATTTTCAATTGTTGTGGACCTAGTGGCACAACTGTTATTATTACTTATTATTATTATGGGATACATATATGTATGTTCTTAGCCTTAGAGGATGGTTTTGCTTGCTTGAATACTTTGCGAAATTTTGGCTTTGTTTTTGTTCCCCCGACTGTGCCCCAGTTGCTTTCTAAATTTGAGGTCAGCTTCAGCTTTCCACCAGTTTTAGGAGCTTTTTATTAATTAAAAATGGCTTTTGTATGAGGAATGTGTCTGG
TARGET=1076,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05478 Ps_contig_mira-and-tgicl-ass_franssen_37987
SEQUENCE=AACTAAATTAAATTTAATTACTATTGCATATATACTAAATTTAGAATTTGAAAAACTAAAACTCATTAAATGAGTTGAAACTACAACATACCATCCACATTGGTTGTTTGATTCATGAACAAACTGAAGTTATCTGGTTTCTTGTTAAAAAGTGTGTTCCTGTCCTATACATCGTACATGGATGGTTGATACACAAGTTAAACAGATAAATGTGGGTTCATTAGTTCAATTAACACACATGCTTTCCTTATCTCAATCACTGGAAAAAAGTAAAACGAAAAACTATATTCCGACTAAAAAATGCCATAAAATTACTGCATATTTCTAACAAGATGTGGCTGCAATTCTCAGCACTCACATGGGGTTACCCCTATTCCAAAAGTTAATAACTTATCGAAGGTTCAGGGAAAAATTAAGAGTCGATCCCTTGATTCCTATATTGAATTGTTTAAATAACTGTTAAGGTTCCCAATTTTTCACAACAGCTCCAGATGACTTGTTATTACATCTGGGCAAGACTGTGCTTGCAGAAGCTCCAAACGGTGGAAAGGTGGAGCGAACTGCAAAGTGTAATCTTCATTTTCTTTGACTGTTCCATAAAGCCAGCATAATACCTCATCCGCAAACGCCAGGCAGACTCCTCCCTGCCAGTAGTTCTTCATTTTTACTCTCTGCGTGATATCTTTGGTTCTAAGCATTTCTGTGCGAACAAGACGGCCTGCATCGTCCCTAAATCCTATACAAATGTACGAAACACCTGCCAGGAATGACTGAATCCAAAACTTTAGCAGTTTCTCTCTCTCAAATTTTTCCTCATTATGATAATTCAACTCGTAACTTGTCTTTAACTCCACATAAAATCTCTTTCCATCGTTAGTCGTATCGCAGCAATCCATCTCAGCACCCATTAAGATACGGTGACGCCCCCTAATTTTGTTTTAAGCACGGAACAAAATTCAACATTGGCATCAACATGATGTATCCCCTCTCCATCAGCTCTTCTAGGA
TARGET=794,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4704
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.340007
PRIMER_LEFT_SEQUENCE=TCCCTGCCAGTAGTTCTTCATT
PRIMER_RIGHT_SEQUENCE=ATCTTAATGGGTGCTGAGATGG
PRIMER_LEFT=641,22
PRIMER_RIGHT=913,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.340
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=273
PRIMER_PAIR_PENALTY_1=0.4704
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.340007
PRIMER_LEFT_1_SEQUENCE=TCCCTGCCAGTAGTTCTTCATT
PRIMER_RIGHT_1_SEQUENCE=CTTAATGGGTGCTGAGATGGAT
PRIMER_LEFT_1=641,22
PRIMER_RIGHT_1=911,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.340
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=0.5670
PRIMER_LEFT_2_PENALTY=0.226971
PRIMER_RIGHT_2_PENALTY=0.340007
PRIMER_LEFT_2_SEQUENCE=CTCCCTGCCAGTAGTTCTTCAT
PRIMER_RIGHT_2_SEQUENCE=ATCTTAATGGGTGCTGAGATGG
PRIMER_LEFT_2=640,22
PRIMER_RIGHT_2=913,22
PRIMER_LEFT_2_TM=59.773
PRIMER_RIGHT_2_TM=60.340
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=274
PRIMER_PAIR_PENALTY_3=0.5670
PRIMER_LEFT_3_PENALTY=0.226971
PRIMER_RIGHT_3_PENALTY=0.340007
PRIMER_LEFT_3_SEQUENCE=CTCCCTGCCAGTAGTTCTTCAT
PRIMER_RIGHT_3_SEQUENCE=CTTAATGGGTGCTGAGATGGAT
PRIMER_LEFT_3=640,22
PRIMER_RIGHT_3=911,22
PRIMER_LEFT_3_TM=59.773
PRIMER_RIGHT_3_TM=60.340
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=272
PRIMER_PAIR_PENALTY_4=0.6520
PRIMER_LEFT_4_PENALTY=0.459263
PRIMER_RIGHT_4_PENALTY=0.192761
PRIMER_LEFT_4_SEQUENCE=GTTCTAAGCATTTCTGTGCGAA
PRIMER_RIGHT_4_SEQUENCE=GCTTAAAACAAAATTAGGGGGC
PRIMER_LEFT_4=687,22
PRIMER_RIGHT_4=943,22
PRIMER_LEFT_4_TM=59.541
PRIMER_RIGHT_4_TM=60.193
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=12.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_05479 Ps_contig_mira-and-tgicl-ass_franssen_38005
SEQUENCE=CAATCATCTATTTTTCYTTTTTCATTAAATGAATCTAAAGTTATTAGTTACTAGATAAAAATTAACAACAAATAGCCTGATAAAATTAAAAAAAACTAAGGCAATTGAAGAGTACATCAGTATATTCATCCTTTCATTCCACTGTTTCCATATTTGCCAACAATGATGATGGAGGAAAACCAACTCCAATCATTTATGTGTTCAATCATACTTCCAACTCCTTGTTAAAAACCTTCAGTAAGCGCAAAAGTTCGACCAGCTAACGACGGTGGCAATGCACTTACAGCTTGTTGAATTTCCTCTACTGTATAACGAGCTGAAAAGTGAATCAAAAGGACTGATTTATTCTGTAACCTATCAGCATGGTTTATTATCTCAGATAAATGAGTGTGTCCATAATCCCTGGCATGTTCCACGGTTATTGAATTGTTAAGAAAGGTGCACTCCAATACAAGAAGTTTCGCCCGCAAGACATCAGTGTTATTTTCGTCACTTATGAAATCTGCTGTGGTGTCTCCTGTGAAAGCAATCTCTGGTTCTGCCAAAGTGTTTGTTATCTCCACACCAGATGACTTCAAATTCTTAATTTCATTTCCAGAGAGACCAATATACTCTGGTTTAAGTTTATTTCTTACAGAGTAAAGTATATAACCCTGGCTGGGTATCACATGATAAGTCCGAAAAGCTTTAACTTTAAGATCGTTTCTCAGAGAAAATTCTTCTCCTACATCCAAGCCTATGAGATTGTGCTTAAGCTCAGATTGATCCATTTTCCTGTGAATCTCAAAGAGTTTCTCTACATCTTCTTTCACTGAAATTGGTACAATAATCGTTGGCGGCTTCATTCGGTATAAACCTCGTGTCGCAACATACATTGGTAAACCTCCAATATGATCCATGTGAGCATGAGAGATTAAAAGAAAGTCTTGAGAAACAGCACGAGGTGGGCAACGACCAATATCGAAGGCTACGTTAAGCTTGGGAACAATGATGCAAGTTTCATGGCCTCCAATGGATAAACCCTCAATTTGGTATTCTCCAATGTTCAAACCCTTTGATTTNTTTGAACAAGACTCTGTTGAATGCTCAGTTACATGCTCCATCAATAATGATTGGAGATGGAAGTGGATTGGTGTGGAAAAGAATCGAGGAATGGCGGTGTGTAGTGGAGCTCTGATGCGACGGAACATCCGGTGTCGGTGGGTTGCAGTTTTGCCGGGGCGGCTAGCGTCGCCGCAGATGGTGTTGACGGATGACAAGACAGTGCGGTTTCCTCGTATCCTAATTATTTGTCTCTTATTTTATTTATTTACCTCTCATCGAAAAGAATT
TARGET=1301,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05480 Ps_contig_mira-and-tgicl-ass_franssen_38010
SEQUENCE=ATGGNAGAAGATGAAGGCTGCAAGTACCTTAGGCTGGAGAGAAGAGCGCCTCAGAAAATGCTGAGGAAGTTTCGTTTGCCAGAGAATGCTAATGTATCGGAAATCACAGCTAAATGTGAGAATGGAGTTCTTACTGTGGTTGTAGAGAAGCAGAGTCAACCTCCAAAATCAAAAACAGTTGAAGTTGCCATTGTTTAAGGGATTGGGTTTAGATCTTTTGTAGTGTTTTGTTTACCTGTTTAGAAGTTTACAGAAGTGGTTTTAGCTTTTGAGTTTGTGGATGTTAAGGTGGAAATTGAAGTGTTGAATCAATGTTTCTAGTTTCTAGTCCAAAATATATATCAATTAGAGGTACCTTAAAATACAGAAAAGTGAATGAAAATGTTACATCTAGGAAAAAAGAGGTACAATGAAACCAACATTGTATTCAATGACATAAGAAACACATTTGTTTGGTTTGGTTTGTAACT
TARGET=451,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05481 Ps_contig_mira-and-tgicl-ass_franssen_38027
SEQUENCE=GATGTGGAACTGTGTAGTCTCTACTTTTGTTTTCTTTACTTTGTAAAGCCTTTACATTGAGATCAGTATGACGAGAGTATGATAGATGAAGCTTACAATAGCCACCATCATAAATGCAATGCCCCTCTAAAGCTTCTCTAGCAGCTGCTGCTGCTGTAACATCAGGGTACTGAACTAGTGCCTGAGTTTGACCTATTTCTTTTACGAACATAG
TARGET=144,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.6422
PRIMER_LEFT_PENALTY=0.650955
PRIMER_RIGHT_PENALTY=1.991211
PRIMER_LEFT_SEQUENCE=CAATAGCCACCATCATAAATGC
PRIMER_RIGHT_SEQUENCE=AGAAATAGGTCAAACTCAGGCA
PRIMER_LEFT=95,22
PRIMER_RIGHT=199,22
PRIMER_LEFT_TM=59.349
PRIMER_RIGHT_TM=58.009
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=105
PRIMER_PAIR_PENALTY_1=2.7141
PRIMER_LEFT_1_PENALTY=0.650955
PRIMER_RIGHT_1_PENALTY=2.063106
PRIMER_LEFT_1_SEQUENCE=CAATAGCCACCATCATAAATGC
PRIMER_RIGHT_1_SEQUENCE=AAGAAATAGGTCAAACTCAGGCAC
PRIMER_LEFT_1=95,22
PRIMER_RIGHT_1=200,24
PRIMER_LEFT_1_TM=59.349
PRIMER_RIGHT_1_TM=60.063
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=106
PRIMER_PAIR_PENALTY_2=2.7851
PRIMER_LEFT_2_PENALTY=0.650955
PRIMER_RIGHT_2_PENALTY=2.134169
PRIMER_LEFT_2_SEQUENCE=CAATAGCCACCATCATAAATGC
PRIMER_RIGHT_2_SEQUENCE=GAAATAGGTCAAACTCAGGCAC
PRIMER_LEFT_2=95,22
PRIMER_RIGHT_2=198,22
PRIMER_LEFT_2_TM=59.349
PRIMER_RIGHT_2_TM=57.866
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=2.8109
PRIMER_LEFT_3_PENALTY=0.650955
PRIMER_RIGHT_3_PENALTY=2.159919
PRIMER_LEFT_3_SEQUENCE=CAATAGCCACCATCATAAATGC
PRIMER_RIGHT_3_SEQUENCE=AGAAATAGGTCAAACTCAGGCAC
PRIMER_LEFT_3=95,22
PRIMER_RIGHT_3=199,23
PRIMER_LEFT_3_TM=59.349
PRIMER_RIGHT_3_TM=58.840
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=2.8109
PRIMER_LEFT_4_PENALTY=0.650955
PRIMER_RIGHT_4_PENALTY=2.159919
PRIMER_LEFT_4_SEQUENCE=CAATAGCCACCATCATAAATGC
PRIMER_RIGHT_4_SEQUENCE=GAAATAGGTCAAACTCAGGCACT
PRIMER_LEFT_4=95,22
PRIMER_RIGHT_4=198,23
PRIMER_LEFT_4_TM=59.349
PRIMER_RIGHT_4_TM=58.840
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=104
=
PRIMER_SEQUENCE_ID=SSR_05482 Ps_contig_mira-and-tgicl-ass_franssen_38039
SEQUENCE=CCACCAGAAGGTTTTTAGTTTGACTTTTGTCACCYTTTTGCTACTATGTGGAATGCCTTCTTTTCGTGGATAACATCATCTTCTTTGGCATATATATATGGGAGGGATGTAAGTTTTCACGAAGAGTTTCTATCAGTTAATGGGAGAGAATATCCTAGCAAAATTGTCTTGACAGATGGTCGATCATCTGAAATAAAAACAAACATTAGCCAGTTGTCTTGCTCGAGCTTTACCTGCAGTTGCTGCTGAGCTCAGGCTGCCAATACCAATATCTACATTAGAACAAGCGGTGGTATTCTTGCTGGATACAATGTCATTTGTGGACGCACTTCCAGCATTCAGAATGAAACAATGGCAAGTGGTTGTTCTCTTGTTAGTTGATGCATTGTCCGTTTGTAGAATACCCACTCTTATCTCATACATGACTGATAGGAGGGACTTGTTTGTCAAGGTTCTGAGTGGTTCACAAATAGGTAAGGAAGAGTATGACGTTTTGAAGGATCTCATTGTACCACTTGGTCGAGCACCTCATTTCTCTTCTCAAAGTGGAGCGTGACAGAGCATAGAACTTCATCATAATTTGCCAAAAGCTTCTACTTAATCACTTTTAATCAAGAGCACAATGTTTTCCTTTTTCTTCTCTTTGGGAAATTATACCTTCCAAGTATTGTAGTATTGGGTAACTATGTTCATAGACTAAAAGGTTGTCCGATTTGTAACTAAAAAAATTGTCCGATTTTACATTTTCGGTATTGATTTCGAGTG
TARGET=90,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05483 Ps_contig_mira-and-tgicl-ass_franssen_38047
SEQUENCE=CTTATGCTTCTGGTTCTTCTTCTTCATCTCCAAAAGGGTTGTGGTCTTGAAGCTCTCTTCCCTTGGAGAACTATGAAGCACAAGTTTTGCGCTGTAGAGAGCCCTGTCCTGCCATGCTTCCACGACTACGAATCTGCCAAGAACTCAGACGTAGAGTCGAGACAGAAGAAACATCGGTCGTCAGCGTACATTCCTTGCACAAATGTGAAGAAAGATGTCTGATGAGTCGTGTCGAGTCGAGTCGTTCTAGTTTCCTTTTCTTTTTAGGTCTAAACCCTCCGTATATTTTGAGTTAAACCATGTGGAATTCGAAGATGCGAAGTTCTTTATATTTTTTGTTCATAAAACTCAAACGCAATTCAACGAATAAGACTCAAACGAC
TARGET=14,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05484 Ps_contig_mira-and-tgicl-ass_franssen_38050
SEQUENCE=TAACCGGGGGGGGGGGGCGGGGNGNTRARTGAAACTAAAGGGCACAATAGGTGGTTGAATAAGGAAAGCTAACTCTGTCTCTGCTATGCACTTGTTTTCTCTCTTTTTGGGTGCTTCTTTTCTCTCCTATTAATTTCTTTCCTTTCATTTTCTTTCCCTTTTCAATCTATCCACAACTATCTTTCTTTTCCAATTTCGTCTAATTTCAACAAACCCTCATCTCTCTTCTCTCTTCTCTCTTCTCTCAACAACTACTTTCTTTCTTTCTCCAGAGAGAAATTGGATTAAAATGGTGAGAGGAAAAACTCAGATGAAACGTATTGAGAATGCAACAAGCAGACAAGTTACATTTTCAAAGAGAAGAAATGGTTTGTTGAAGAAAGCTTTTGAACTATCTGTTCTTTGTGATGCTGAAGTTGCTCTTATCATCTTTTCTCCAAGAGGAAAACTTTATGAGTTTTCTAGCTCCTGCATGCAGGACACGATTGAACGGTACCGTAGAAATACCAGAAGTGCTCAACCATTGCAAAGATCAGATGAACAAAATATGCAGAATTTGAAGCAAGAAACAGCAAGTTTGATGAAGAAGATTGAACTTCTCGAAGCCTCGAAACGGAAACTCATGGGAGAAGGTTTAGGTTCATGCTCCTTAGAAGAACTTCAACAGATAGAACAACAGTTGGAAAAGAGTGTAAGCACTGTTAGAGCAAGAAAGAATCAGGTTTACAAGAATCAAATTGAGCAACTTAAAGAAAAGGAAAAAGTCCTACTTGCTGAAAATTCAAGGCTCTCTAAGCAGCCGCAGCCGCAACTACTGCAACTACCACCACCGCCACCGCCAAATGATCATCCGAGAGAAAATCAACCGTACGCAGAAAGCAGCAGTCCGAGCTCAGATGTGGTGACTGAATTGTTCATTGGCCTACACAGGTCTAGTTGAACATAATGATATAATCATCTAGTGTATGTTTGGTTTTACATTAATTCCAATGCATCTGAATTAACATTTTGAAACTACTTCCCTTATTGCATTGGAATTTGTTCAACTTAAGAACAAACATATATATGCTTAATACTTGCCTTATATACGTGCAAGTGTAGAGTAATGGGGAAAAGCCCTAATGTCTCCAATGTATTTATTTGTTGAACAATGTGATGCACTGAATATATTTTCATCATTTTGTTTTTGTCAAATTGCTGTAAAAATTATAGATGGTCTTAAAATATATAAAATTAAGACCATTGCATCGTAAGACTATTAGAGACAAAAGTGTTGTCCTGGAACATCATGGAACTTTCAAGTTGGTAAGCTTGGTAATTGTACTTGGATTTCTGATTAAGATCTTATCTCAGGGTTGATTTGTGGGGAAGGTGTAG
TARGET=255,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05485 Ps_contig_mira-and-tgicl-ass_franssen_38091
SEQUENCE=CNCCGCNCACAAACNCAAACCTTACCATCCCTACCACCACCACCACCCACAACCACCTTTCGTAACCCCAAACGACTCCGAAGCCGTAATAGACCTTTCCCNTTCYCTTCCTATTCCAACATTCGTCAATTCGTTTCCTCCGGTGAAGCGGCATACCGCGATTTACAGACCTTGTTCACTCTCGACGAAAATCGCAGAGTCGTCGTTTCGTGCCGGCCTTCCACTATTCACTTCGTCGGAACTTCAGCTGCTTTGACTCTCGTAGCGTTTTCTGTTTTTAGAGTGTTGATTGAATTGGTTTCTAGGTTAGCGCCGTGGCGGCGAAACGCGTCAGGTTATGGTAAATCTATGGTGCGCAGAGACCGGAGTCTTGGAGGGAAAGAGGTGGTTGTTGGATTGGGTTCCAGGAGCGGCGTTACGGCGCCGATTAAGCGGTCTTTGAAGAACAATAAGGTTGCAGTTCAAAGGAAATTGCCGAAATGGTGGCCAATTGTTAATAATCCCAATCACAGTGATTTTGATTATGACCTAAA
TARGET=33,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05486 Ps_contig_mira-and-tgicl-ass_franssen_38101
SEQUENCE=CCTACCAATGAGTCTGTAGTTGTCGTCGTACGGCACTTAGTTGTTTTTTGGATCAGTGATGACATATCCATTGTTACGGTACAAATCAATTTAAATTTGTGGCATAACGTTGAATCTTTCATTTTCATAACAGTACTGGAYATATTTGGGATANGTTGTGTTTGGGGTAATTTTTCGGTTCCATCTTAGATATGTATTCATTTTGCTTTGAACTCTGCATTGCTTATGTTAGTTATCCTTCTGTAGCAGGATGTACCAGTAATTTTCTTTTCTTTTCTTGAAGTGGCTTTACTTGGCTTTTATTTTTCGGTTGCAATTACTACTCATTTTCAATTCAAATTATTCAGTGATTGGTATTATGTTTGTATGTTGGAATTCTAGTAATCCTGTAAATAATCATAACGTGTTCTATTTTTCCTTTGCACTATATAATATCTATTTCGGTTTCAATTTGATTACTTTGCGTATCGTCTACATTATGCGTTTCAATAACGAATGCTTGTATGTCGAAGTTTTGTTTTGAGTTATATGTTGGATTTTTAATTGACATGGTAATGATCCTGTAATTATGATAATTACACGTACTATTTTACTACTGTATTGTTGAGTTTTGATTACATATGTGTTTCGATTGCGTATCCATTTCGGTTATAGTTTTGTAAATTACTGATAATTCTCTTTGGTTGATTCTGTTAACTCTATTTTTTACAAGATGATTAAGTGATTGTTGTTCCCACTTGTTTAACTGATGTGCTGAACTAAGTGTATAACTTACCGGATATTTTTATGCTTGTAATGTTATCCATTAGTTTTGACAATGTTGAAATTTTGTGCTTTAAAATTTAAGTTCTTTTCATTAAAAGCCAACACTTTTGTTTTGCTGCACATTTTAATGGGTAGGG
TARGET=263,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05487 Ps_contig_mira-and-tgicl-ass_franssen_38102
SEQUENCE=AAGCTTATTGTTCTTTCTRNGATAGTACTTTCTTTTCTTGTGTTTGTCACTACAGTACAACCAATCAATCATGGATTCAAGAGTAGTTCAAAGTAAAGTGGTTGAGAACAAGGATGAACAAAACCTTGATTCACAACAAGTGACACATGATGCAGAAGAGCACAATTCTATTGAGCCTGAGAAGAAATCTGTTATGAGTAGGGTGAAGGCGAAGGCTAGGAAAATTAAGAACAGTATAACGGGGCATGGTCAACAAGCACCTGATCAAACCAGTGGACATAACACTGAAATTCAGCATACTCCTGAGGATGATGAAGAAGATGATGATGATGATGGTGACTTGGATGAGGGTAAAGAAAATGTTCAAGAGCCACTACGCCATCATGCACCAAATTATGGTAGTAGAGATGTTAAAAGTGCTACACCTACAACTGAATATGAGAAAGTTGAAAATTTAAGAAATTTGGTATTTGGTTTGGAAGGTTCAAAAGTTGTAGGAGAAGAACCTCATCATGATCCATTCACTGAAGGTGTTTCTTCAAACACTGAAACCAATCAAAGCATAGCCACTGACAAGGCCAAAACATTTGATCTGGAAGATAAGCCAGACCAATTCAAGACCGATTTCGAGAGGCCTATAGTTTCGAAGGAAGATTCTCAAGAACAAGGAAGTGGAACTGATGCATATGATCTTCCCAATTATAAAACCAGAGACACTGATCCAAATCTTGAAGTTTATGATAGTGAAGATGTTAAAAGTGCTACACCTAAATTTGAATCTGAGCAAGTTGAGAATTTAGGAAATTCAGGATTTGATTTTGAAGGTGCAAAAGTTAAGGGAGAAGAAACTCGCCGCGATTCACTAGATGAAGATGTTTCTTCAACAACCACTGAAACCAATCAAAACATAGCCAAACCATTTTCTGTGGAAGATAAGCCAGATCAATTCAAGGCCAATTTGGCGAGGCTAGGATTTTCGAAAGACGATTTTGAAGACCGAGTAAATAGAACTGGGGCATATTCTCCTCTCAAGCATCAAACCGATGACACTTATCCAAGTGAAGATGTTAAAAATGCTACCCCTCCACCTGAATACAAGCAAGATGAAAATTTCGGAAATTCAGGAACTGGTTTGAAGGTGACAAAAGGTTAAGGGAGAAAGAACCTCGTCACGTATTCACTTATTTGGAAGGTTGTTTTCCTTACAAACCAACCACTAGAAACCAATACAAAACNTACGACCCAAA
TARGET=320,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05488 Ps_contig_mira-and-tgicl-ass_franssen_38103
SEQUENCE=GTTATTAATATTTATAATATTTATAAACAATTTATTCTTAATCAATACAAATCCAATCATGTAATATACCACACCAAAATAAATAAATGAATAGTTCAAGTAGGTAAGGAAAGCAACAAAAAATAAAATAAAAATACACACAAACACTTCAATCGTATTATTCAAGTTTTATCTCCTTATTGCAGCGGCACCATGAATAGCTGCTATGTAGAAAAGCTGTGTAGCTGAAAGAATAATCAGAAAAGCCTCCATTGTTTTCAGACGAGAATTTCTGACGTTCAGCTGAATCTCTTTGCACCCAAAACCCATGGCAAGAACGGTAAAAGCCCATGCAATGGCAGCAACTGAAGCTGCAGATGGCAAGCTTTCTGAAGTCCAGGAACGGATATGATTCAGTCCTGAAATCAATGATCCAACCCCAACAACTCCAGCAATCAAAGCAAATGTAACAAAGAATCCAGTGGCAGCATTTCCCATAGGGAAGTATATGGGCGAAAAATGAGCCGGAAGATCGAACCCTGGACCTATAATAAAACCATGATCTATAGCTCTATTCATGGCCCATCCACCAATGCCCAAAACTATGACATACATGCAAAAATTCAGCCCCAAAAGAAGAGTTGCAACTGGCTTCATTTGCTCATTTGCCATCTTTCTGCTGTGTGGTTTTTATCCAAATAAAAAGCTGGTTGGAAAAGGAAATATCAGAAAGACAAAAAGTTGTGAAGGAAAATCTTAAGCACAGGACTAGGATATGATACAAATGTTTTGTGTC
TARGET=77,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05489 Ps_contig_mira-and-tgicl-ass_franssen_38121
SEQUENCE=ACTCTTCTTTTCATCTCTCTCTCCAAACCCTTCTTTTCTCTCCCNATCGTTTTCTATTTTCCATTCTTCCTTTTTCCTTCTCTTGCTCTCACATAGCCTCAAAACCTATACATATAACCTTCAACCATCAACTATGGCTCCTCCTTCAGATATCAACCATCTCTCTAACCCTTGCATATATGGGGATTTTTCTTCTTCATACTCAGAGAGAAAATCTGGTTTTATGAAATGGTTTGGTAAGATTTTCAAAATCGGGTCAAATAGAGGAAGAGGTCGGGGG
TARGET=14,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05490 Ps_contig_mira-and-tgicl-ass_franssen_38125
SEQUENCE=ATAAACCAATGTTTCATTTTCTTCTTCTATCTTGAAGTTCACCCTCACCCAATAGAATAAACATAAACTACAATATATAGCAATTATACACTTCCAATAGGTTGGTGGCTTCTCACAAGATAGTTTCAGTTCAACATAGGGATTTCACACAATATCTCTAAGTACTAAGACAAAACTTATAATTTTCCATATAGTACATGTTCAGACAAAAATGAGTGTCACTAGCTTATCTAATTCTATGCTAGGAAATGCCAGTCGCAAACTACAAACAACCTGAAATAAGAATCAACGATGAGGCGGCTGTTTGTTGGGCATGCTCAGCCTTTTAAGGCAAGTAATGAGATAGAACTGAATACTCAACCAACACCGATATCAAAAACGACAAGGTTAAACTCTCAACATGTTTGATCAGAAAAGCTTACTAAGACCATGAAAAAACCGATGATTTCTCCTTTTTGAGTGTCTTCTAGATTCTTTTGTCATCACATATGGTACAAAATGTACCTGAGAATTATTCCTCACACCCAAATCTTGAAGCGCATCATCATCATTTAAGAGCTTGTTATTATCAAATGACAAACAATAATTAGCCCACACACTCTTCCAAGAAATGTGACGATGACCCATGCTAGATTGTTCCATGTAATTCACTTTCTTCTTGATAGCAAGTTTCAAAACCTTCACCGTTGCCGTATTCATAACAATCACATCCAACGAAGTTGAATCCAATTTCAAAACGGAAATTCGCATTGCACTACCCAATTCAAGTCCAATCAGTGTTTCCACATCTTCCAAAGATGGATTCTTTGGTACATCACTGAGTATTGGATCTTCCAATAAAGCTTTCAACACCTTCTCCTTCTTCAAATCGCTCTTGTATTCTGCACCTTCACCCATCACACCACACTAAAAAATGAAGCGGTGGCAATGAAGGGTGGCTGAATAGAGAAATTTGTGTGATTGGAAATGGATGAACAGAAGGTGGAGTTACGCCGGAGGAGAGAAGAAGAGTTTGGGTCGATTAATTGAACGGATAAGCGGCAAAAAGAAAAAACAAAGTCACTCTGTCGTTTGTTATTTACCTTTACTTTTGTTTTCTTTTT
TARGET=540,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0526
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.022341
PRIMER_LEFT_SEQUENCE=CTGAATACTCAACCAACACCGA
PRIMER_RIGHT_SEQUENCE=GTCATCGTCACATTTCTTGGAA
PRIMER_LEFT=348,22
PRIMER_RIGHT=622,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=59.978
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.0906
PRIMER_LEFT_1_PENALTY=0.068302
PRIMER_RIGHT_1_PENALTY=0.022341
PRIMER_LEFT_1_SEQUENCE=CCGATGATTTCTCCTTTTTGAG
PRIMER_RIGHT_1_SEQUENCE=GATTCAACTTCGTTGGATGTGA
PRIMER_LEFT_1=438,22
PRIMER_RIGHT_1=725,22
PRIMER_LEFT_1_TM=60.068
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.0906
PRIMER_LEFT_2_PENALTY=0.068302
PRIMER_RIGHT_2_PENALTY=0.022341
PRIMER_LEFT_2_SEQUENCE=CCGATGATTTCTCCTTTTTGAG
PRIMER_RIGHT_2_SEQUENCE=GTCATCGTCACATTTCTTGGAA
PRIMER_LEFT_2=438,22
PRIMER_RIGHT_2=622,22
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=59.978
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.1615
PRIMER_LEFT_3_PENALTY=0.139129
PRIMER_RIGHT_3_PENALTY=0.022341
PRIMER_LEFT_3_SEQUENCE=CTCAACCAACACCGATATCAAA
PRIMER_RIGHT_3_SEQUENCE=GTCATCGTCACATTTCTTGGAA
PRIMER_LEFT_3=355,22
PRIMER_RIGHT_3=622,22
PRIMER_LEFT_3_TM=59.861
PRIMER_RIGHT_3_TM=59.978
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=268
PRIMER_PAIR_PENALTY_4=0.1704
PRIMER_LEFT_4_PENALTY=0.068302
PRIMER_RIGHT_4_PENALTY=0.102129
PRIMER_LEFT_4_SEQUENCE=CCGATGATTTCTCCTTTTTGAG
PRIMER_RIGHT_4_SEQUENCE=CAACTTCGTTGGATGTGATTGT
PRIMER_LEFT_4=438,22
PRIMER_RIGHT_4=721,22
PRIMER_LEFT_4_TM=60.068
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_05491 Ps_contig_mira-and-tgicl-ass_franssen_38127
SEQUENCE=AAATTTNATTCCATGAGCGTTCTATCAAATAAATAAATAAATAAAATCCATGACCGACACTTGAGAAGTACAAATGGTAGAGGATGTGCAAAAAACTGCAAACTCCTCTTGGGTGCAAAAATGCGTTTCCACTATTTTCACAGAAGACATAAAATACACGAGTCCCTAGAGAAGTATCACTTGCATTTAACCATAGACAAAGATAATATTTGCACAAACACACACGGATTCATAATGCTGAATACCACCCATCAAAATTTTGAGGATCTTGCACGTTTGATACTGTTTTCTCTTTATCTGTCTGTATATTGAATCAATGTAAACAACATCCTGTATTTCCATTAACATGATTCCACCGCTGGAAGAACTGTGCCATATGCAACAGTAACTATATCTGCAAGTCCTCTTTTGGAAATCATTTACGCCATGCTAGTATCCAAAGGATAACTCCCTAGCACTCGCAAGAATGTAGCAAACTCCATTAGATGCCTTAAGGCATTTTGTGCATTCTGATCAGCCATTGATGCTTCAAAATCAACATAGAAAAGATAATCAAAGTACCTATTGTTGTTATCATCAGATGTTCTAAGAGGTTGCTTCCGCAAAGGACGGCTTTCAATCTTTGAAAGATTAATTTGACGCAGCGCAAAAACAGCAAGGGCCTTGAAAAGTATCCCAGGTCCCTCTTCTAAGGAGAAAACTATGCTTGTCTTAAATGGCCTGTCTGTGCCTGGAAGTATAGGTTCTCGCGCCAACACTAAAAATCGAGTGATATTATCACTATCATCCTGGATGTCTTGAGCAAGTATATTTAAGCCATAGATCTCTGCGGCAGCTGAGCTAGCAACAGCTCCTGCATCTTGTAGTTTATGGAAAGCAACATGCTTTGCAGCACCGGCTGTATCATCAACAGCTTCTCTGACTAAGCCCAATCCTGTCAAGGTGTTCTCACATTGTGCAAGAGCCTGAGGATGACTAAGAACACGCTTCAAATTCTCAAGTTTAACACCATGGTTGGCCATCAAACAATGATGGACTGCATATTTTACTTCTCCTACTATATGTAGCCTGTGCCTGAGTAAAAGGTCATAATTCCTGTGGATGCTCCCTCCTAAAGAATTCTCAATTGGCAAAACTGCTCTGTCCACAAGCCACCTTTCAACAGATTCAAAAGCGGTATCAAATTGTTCACAAGGCACAGCTTCACAATTAGGGTAAGCTTTTTGTGCCGCAGATTCACTGTAAGCACCTTGAACTCCCTGATAAGCAACACGAAGTCCTGAACCATCGGAAACAGCACTAGATAACTGATTCGAAGACAAAGGCCGAGGAAGAGCGTGCGGATCTTTGGAAACGACGTCGTTTGAGACGGCGGAGACGGGGCTAGTTCGCAATTCAACGGAATGCGAAGTGTCGTTGAGGGAAGCACGAATGCGGAGGTTACGGTGTCGTTTCGGGTGAAGGTTGAGATTGAGAGACGGAAGAGGTTTGACGGAGTCAAAGGGGGAAGACTGACGGTGGAGATTGGGTTGAGAATGAGAGATGATTCGGGAAGATGCCATGAATGGCGTGTTAGGTACGGTGACTCTGTCACTGCTGCTTGGTTAAGTTTGAAGTTACGATCTCTCCTTTCCCCGYGTTNCTAGTTTTGTTATTTATATGT
TARGET=27,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05492 Ps_contig_mira-and-tgicl-ass_franssen_38128
SEQUENCE=AGCCCTGGCAGCACRGATCTTGGTCAAAAAAAAAATGGAACCCAATGACAACCAATTAAGCTCTTTCTTCCACCACCACAACCACCAACAACAGCACCATCACCACCCCCAACACCACCAACAACACCATCAGGGAAATTCCACCACTCTAGCCTCCGCCACCACCACCACCACCACCACCGCTTCTCCCACCAACGGCCTCGATGGATCCCACATACTCTACCCTTCTGCTGTCTCCTCTCAGCTGGAACCAGCTAAGAGAAAAAGAGGAAGACCTAGAAAATATGGAACTCCAGAACAAGCCCTAGCTGCTAAAAAGCTTCTACGTCGTCGTTTTCACCTACTTCTGACAACAACAACAACAACAACAGCAACAAGAATACCAACTCTTTCATTCCTTCTCCTTTTTTCACTTCTAAGAAATCTCATTCTCTCTCTCTAGGCAATGCAGGACAAGGTTTCAGTGTACATGTCATTGCTGTTGCTGCGTGGTAGG
TARGET=159,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05493 Ps_contig_mira-and-tgicl-ass_franssen_38128
SEQUENCE=AGCCCTGGCAGCACRGATCTTGGTCAAAAAAAAAATGGAACCCAATGACAACCAATTAAGCTCTTTCTTCCACCACCACAACCACCAACAACAGCACCATCACCACCCCCAACACCACCAACAACACCATCAGGGAAATTCCACCACTCTAGCCTCCGCCACCACCACCACCACCACCACCGCTTCTCCCACCAACGGCCTCGATGGATCCCACATACTCTACCCTTCTGCTGTCTCCTCTCAGCTGGAACCAGCTAAGAGAAAAAGAGGAAGACCTAGAAAATATGGAACTCCAGAACAAGCCCTAGCTGCTAAAAAGCTTCTACGTCGTCGTTTTCACCTACTTCTGACAACAACAACAACAACAACAGCAACAAGAATACCAACTCTTTCATTCCTTCTCCTTTTTTCACTTCTAAGAAATCTCATTCTCTCTCTCTAGGCAATGCAGGACAAGGTTTCAGTGTACATGTCATTGCTGTTGCTGCGTGGTAGG
TARGET=350,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05494 Ps_contig_mira-and-tgicl-ass_franssen_38128
SEQUENCE=AGCCCTGGCAGCACRGATCTTGGTCAAAAAAAAAATGGAACCCAATGACAACCAATTAAGCTCTTTCTTCCACCACCACAACCACCAACAACAGCACCATCACCACCCCCAACACCACCAACAACACCATCAGGGAAATTCCACCACTCTAGCCTCCGCCACCACCACCACCACCACCACCGCTTCTCCCACCAACGGCCTCGATGGATCCCACATACTCTACCCTTCTGCTGTCTCCTCTCAGCTGGAACCAGCTAAGAGAAAAAGAGGAAGACCTAGAAAATATGGAACTCCAGAACAAGCCCTAGCTGCTAAAAAGCTTCTACGTCGTCGTTTTCACCTACTTCTGACAACAACAACAACAACAACAGCAACAAGAATACCAACTCTTTCATTCCTTCTCCTTTTTTCACTTCTAAGAAATCTCATTCTCTCTCTCTAGGCAATGCAGGACAAGGTTTCAGTGTACATGTCATTGCTGTTGCTGCGTGGTAGG
TARGET=430,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05495 Ps_contig_mira-and-tgicl-ass_franssen_38131
SEQUENCE=GGGGNNATCTTCTTTATTCATCATTCTGCTTTTTAATACAAATCTTCCATTCATCTCAGTTTCATTTCTTTCTTCTATATTGATTTTGTTCAAATAAAAAAAGTTGGNTTTTAATATTTATCTTCATCTGGGTTGAAGAAATTTTGGTTTCTAGAAAAAAAAGGAAAAATTTCTTGACTTTCTGTGGAAATTTAAGTGGGGTTTGATTGAATCGAGGTCCAGGTGGATCAACTTTTTCTGCTCAGCTATAGGAGAAGAAGAAGAAGGTTAAAAAGGTATTTGTGGCTGCAGCATACTTATATGAGCTTTGATATAGTCCAATATGATAGGATTTGATGGAGTCTAAGTGTATCCGAATGGAGTGAATGCTGTATTTGTATTCGGAATTGTAAAAAACACATCATTAAAAGTGGAAGCGGTTGTTATTATTTTAATTTTGATTTAACTGTATCGAAAT
TARGET=253,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3715
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.347935
PRIMER_LEFT_SEQUENCE=TCCAGGTGGATCAACTTTTTCT
PRIMER_RIGHT_SEQUENCE=AGCATTCACTCCATTCGGATAC
PRIMER_LEFT=217,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.348
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.3765
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.352947
PRIMER_LEFT_1_SEQUENCE=TCCAGGTGGATCAACTTTTTCT
PRIMER_RIGHT_1_SEQUENCE=CGCTTCCACTTTTAATGATGTG
PRIMER_LEFT_1=217,22
PRIMER_RIGHT_1=417,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.647
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.3871
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.363508
PRIMER_LEFT_2_SEQUENCE=TCCAGGTGGATCAACTTTTTCT
PRIMER_RIGHT_2_SEQUENCE=CCGCTTCCACTTTTAATGATGT
PRIMER_LEFT_2=217,22
PRIMER_RIGHT_2=418,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.364
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.3871
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.363508
PRIMER_LEFT_3_SEQUENCE=TCCAGGTGGATCAACTTTTTCT
PRIMER_RIGHT_3_SEQUENCE=ACCGCTTCCACTTTTAATGATG
PRIMER_LEFT_3=217,22
PRIMER_RIGHT_3=419,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=60.364
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.4032
PRIMER_LEFT_4_PENALTY=0.209637
PRIMER_RIGHT_4_PENALTY=0.193532
PRIMER_LEFT_4_SEQUENCE=TTCTGTGGAAATTTAAGTGGGG
PRIMER_RIGHT_4_SEQUENCE=AAGTATGCTGCAGCCACAAATA
PRIMER_LEFT_4=179,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=60.210
PRIMER_RIGHT_4_TM=59.806
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=119
=
PRIMER_SEQUENCE_ID=SSR_05496 Ps_contig_mira-and-tgicl-ass_franssen_38132
SEQUENCE=AGATTGAAGCAGTGATGGCAGGGGCACCATGGCCGGTCAAGAAGACATCTACTACTGTTGAAAATGGATTGGAAAGCAAAGCCTGACTTCAAGCATCTTTAGACCTGAAAAGCTGTCATTTTCATCTTTTTTTATTTATTTATATAAAAACAGAGAAAATAACTCCCTCTCTACCCTACACCTTTTCAATATTTCCTTTGGTGCTTAAATGATATGAAAAGAAAAGCTTAATGTAATCATAGAAACAACGAAAAATTGAAGTAATTACTGCTTCTAGTTCTTNTATTAAGTATTGGTTTTCGT
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.7011
PRIMER_LEFT_PENALTY=1.255555
PRIMER_RIGHT_PENALTY=1.445540
PRIMER_LEFT_SEQUENCE=ATTGGAAAGCAAAGCCTGACT
PRIMER_RIGHT_SEQUENCE=AAAAGGTGTAGGGTAGAGAGGGA
PRIMER_LEFT=67,21
PRIMER_RIGHT=185,23
PRIMER_LEFT_TM=60.256
PRIMER_RIGHT_TM=59.554
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=2.7011
PRIMER_LEFT_1_PENALTY=1.255555
PRIMER_RIGHT_1_PENALTY=1.445540
PRIMER_LEFT_1_SEQUENCE=ATTGGAAAGCAAAGCCTGACT
PRIMER_RIGHT_1_SEQUENCE=GAAAAGGTGTAGGGTAGAGAGGG
PRIMER_LEFT_1=67,21
PRIMER_RIGHT_1=186,23
PRIMER_LEFT_1_TM=60.256
PRIMER_RIGHT_1_TM=59.554
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=52.174
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=2.8223
PRIMER_LEFT_2_PENALTY=1.376776
PRIMER_RIGHT_2_PENALTY=1.445540
PRIMER_LEFT_2_SEQUENCE=GAAAGCAAAGCCTGACTTCAA
PRIMER_RIGHT_2_SEQUENCE=GAAAAGGTGTAGGGTAGAGAGGG
PRIMER_LEFT_2=71,21
PRIMER_RIGHT_2=186,23
PRIMER_LEFT_2_TM=59.623
PRIMER_RIGHT_2_TM=59.554
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=52.174
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=2.8223
PRIMER_LEFT_3_PENALTY=1.376776
PRIMER_RIGHT_3_PENALTY=1.445540
PRIMER_LEFT_3_SEQUENCE=GAAAGCAAAGCCTGACTTCAA
PRIMER_RIGHT_3_SEQUENCE=AAAAGGTGTAGGGTAGAGAGGGA
PRIMER_LEFT_3=71,21
PRIMER_RIGHT_3=185,23
PRIMER_LEFT_3_TM=59.623
PRIMER_RIGHT_3_TM=59.554
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=115
PRIMER_PAIR_PENALTY_4=2.8578
PRIMER_LEFT_4_PENALTY=1.412222
PRIMER_RIGHT_4_PENALTY=1.445540
PRIMER_LEFT_4_SEQUENCE=AGATTGAAGCAGTGATGGCAG
PRIMER_RIGHT_4_SEQUENCE=AAAAGGTGTAGGGTAGAGAGGGA
PRIMER_LEFT_4=0,21
PRIMER_RIGHT_4=185,23
PRIMER_LEFT_4_TM=60.412
PRIMER_RIGHT_4_TM=59.554
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=47.826
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_05497 Ps_contig_mira-and-tgicl-ass_franssen_38138
SEQUENCE=GGTTGCTCGTTTGAAGGGTGAAGAGATGGAAAAGCGGTTTTTGATATGAAGTAGATGTAGAAGATTGAGAACACTACAAATGCGAAATCTTGAGGACTAACCATGCCACTGGCGGATAAGACAACAACAACAACTAAACAATTGAGTTGTCGGAATGAGAGGAAAGTGGATTTGTTTTGTGGTTTGGTGGTTGGTTTGTTGAGGTTGTTGTCCTTGAGAGATTGGTCGTGTTGTTCTTTGGTAACAATTTCTTCTTGTTCTTTTGGTAGGCTGATGTCACAGCCGGTTGGTCCAACTCCACCAGACATGTTTTTGAGGTTATGAGTCAAAGGAAAATGTTGTAGTTTTTTTCTTAGAGAGAATTGATGGGAGCTTTGTGAGAGAAGAAAATTAAGAGAAGAAACTGCAAGATAAGGTTAGATTAAGTTATGGGGTGGAAATGGTTTACAAATGTCATGTAAAGAGGTTAGGTTNGTGGCTTGAGACACT
TARGET=121,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2939
PRIMER_LEFT_PENALTY=0.248820
PRIMER_RIGHT_PENALTY=0.045112
PRIMER_LEFT_SEQUENCE=CTCGTTTGAAGGGTGAAGAGAT
PRIMER_RIGHT_SEQUENCE=CAAGGACAACAACCTCAACAAA
PRIMER_LEFT=5,22
PRIMER_RIGHT=215,22
PRIMER_LEFT_TM=59.751
PRIMER_RIGHT_TM=60.045
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=0.3352
PRIMER_LEFT_1_PENALTY=0.248820
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=CTCGTTTGAAGGGTGAAGAGAT
PRIMER_RIGHT_1_SEQUENCE=GACAACAACCTCAACAAACCAA
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=59.751
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.3648
PRIMER_LEFT_2_PENALTY=0.248820
PRIMER_RIGHT_2_PENALTY=0.115965
PRIMER_LEFT_2_SEQUENCE=CTCGTTTGAAGGGTGAAGAGAT
PRIMER_RIGHT_2_SEQUENCE=TCTCTCAAGGACAACAACCTCA
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=220,22
PRIMER_LEFT_2_TM=59.751
PRIMER_RIGHT_2_TM=59.884
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=216
PRIMER_PAIR_PENALTY_3=0.4169
PRIMER_LEFT_3_PENALTY=0.248820
PRIMER_RIGHT_3_PENALTY=0.168114
PRIMER_LEFT_3_SEQUENCE=CTCGTTTGAAGGGTGAAGAGAT
PRIMER_RIGHT_3_SEQUENCE=CTGTGACATCAGCCTACCAAAA
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=281,22
PRIMER_LEFT_3_TM=59.751
PRIMER_RIGHT_3_TM=60.168
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.4341
PRIMER_LEFT_4_PENALTY=0.248820
PRIMER_RIGHT_4_PENALTY=0.185267
PRIMER_LEFT_4_SEQUENCE=CTCGTTTGAAGGGTGAAGAGAT
PRIMER_RIGHT_4_SEQUENCE=CTCAAGGACAACAACCTCAACA
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=59.751
PRIMER_RIGHT_4_TM=60.185
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_05498 Ps_contig_mira-and-tgicl-ass_franssen_38139
SEQUENCE=AGAATCGACTATATATATCCATATAAATTCTCTCCATAGTTTGAACAAGTAGCTTCCCTTCTCCCACTGAGAAAGCCTCTAAAAAATGAATGAATCAGTGGACAGTTTTCAATTGGAACAAAGAGTCAAAACAAACATATAACTTTGTTCCATTCAATAATACATCAGTGTAATAGCAGTGACTAATTACCAAATACAAAGAAAGATCTTCAAACATTAATATCTGATCGCTTTGTTCTACTTGAAGAACTCAGTTCTGAGGAACCAGACAAAGAAAACCTAGGGTAACTGTAGCCTTCAGAGACTGAAACTGGCTGTTGAGGATAGAGTGTGGGTGGTGGAGTTAAATTGCGTTGAGCCGGAAGTGGGGGAAGCATGTTGTACTTGTATGGATCACTGGCTCGACTTGTAATATAAGACTGATCTTCTTCGATAAAACTTTCATCTATCCGCTGAGAAGATCTTTGAGGTGAAGGAATGGACCATGCAGGTGCAGGTATTTCATCTGAGAAGTATCTTCTCCTAATCAACATGCGGAAAAATTTAATCAAAGCCTCCTTATCATACCTAGCATCTTTTGCTGCTGCAATAAGACCTCCACCACTCGGAAAACTCTCGGTCAATGGAAGCTCCTCGCTGGATGGAAGCAAACCCTGCTTCTCGACCCACTGGCGTGCAACATCAACAAGATTCCCACTGCTTCCTCTTGTACCCTCTTTTGCAACAATATCAGCTTTGTTACCAATAACAATATACGGAACAGGAAGACCACCAGGGCCACCAGATCCCAAAGGAGCTGAAAATGTCCCAGTTGCAGCAATCTCAGCTGCCCACTTCTGCAAGCTAGTCTTTGTTCTTCTCTGTGAAAGATCATGAACAAAAATTACACCATTAATCTGTGAATAAAACAGAGATCGGCAATCTTTGTAACGTTCATGTCCTGAGACATCCCACAGTTCAACAAAGAAATCTCTTTCAGAATCACCTTTGAGGCTACTTGAAGAGCTGCCAGAATTTCCATAAGTAGTATGCTTCACATCTACTGTACAACCAATTGTCTGAGAAGGACGAGCAACGGGAGAACCTTTAACAATAAGGTGAACTAAAGAAGACTTCCCAACACCTGAGTCACCAACAACGAGCACTCTGACTTGTCCACAAAGCACCCCACCGTTGTGTTCTTTATTCTCCCTCTCACGTTCCCTCCAGAACATGATTTCCAAACCCTCCAGGCCTTCTTAACAAAAAACAATAAGTCCAAATTCAAACAGAAAAACGTTTTCTTCTCGCTAAATATACACTAATCAAGTTCCGTCAAATAGATTCAACCACCGATTTTTCAAAATGGCTGTATCGAATTTCGTTCCAAAATCCATGTCACATGAGAATCACAACGAGATCTGGAAAAGAGATTAAAACCCCATAGTTACGCATCTCTGAAGATAGAAAGGAAAAAACCATAAAAAGCGAGAAAATGAAATCAATCACTTCAGCAACGGATCTGAATAGTTGAGAAGTGGTGAAAAGGATTGGGATGAAGATTGATTTGGATGTATGCAGTGTCTATGGGTGAGTAGAAGAAAGAGTTGAAGAGTTTGCAGAAATGGTTTGGTTTGGATCAAGGTGGAGGAACCCTTGAAGGAATGATGATGATGATGCGATCTCGATACTCATTCGTGTAAAACACGCGATT
TARGET=1644,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9530
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=1.929417
PRIMER_LEFT_SEQUENCE=GAGAAGTGGTGAAAAGGATTGG
PRIMER_RIGHT_SEQUENCE=ATCGCGTGTTTTACACGAATG
PRIMER_LEFT=1511,22
PRIMER_RIGHT=1691,21
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.929
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=2.0590
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=2.035468
PRIMER_LEFT_1_SEQUENCE=GAGAAGTGGTGAAAAGGATTGG
PRIMER_RIGHT_1_SEQUENCE=GCGTGTTTTACACGAATGAGTATC
PRIMER_LEFT_1=1511,22
PRIMER_RIGHT_1=1688,24
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.965
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=5.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=2.1070
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=2.083428
PRIMER_LEFT_2_SEQUENCE=GAGAAGTGGTGAAAAGGATTGG
PRIMER_RIGHT_2_SEQUENCE=GCGTGTTTTACACGAATGAGTA
PRIMER_LEFT_2=1511,22
PRIMER_RIGHT_2=1688,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=57.917
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=2.1357
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=2.112158
PRIMER_LEFT_3_SEQUENCE=GAGAAGTGGTGAAAAGGATTGG
PRIMER_RIGHT_3_SEQUENCE=AATCGCGTGTTTTACACGAATG
PRIMER_LEFT_3=1511,22
PRIMER_RIGHT_3=1692,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=62.112
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=2.1770
PRIMER_LEFT_4_PENALTY=0.247631
PRIMER_RIGHT_4_PENALTY=1.929417
PRIMER_LEFT_4_SEQUENCE=GAAATCAATCACTTCAGCAACG
PRIMER_RIGHT_4_SEQUENCE=ATCGCGTGTTTTACACGAATG
PRIMER_LEFT_4=1476,22
PRIMER_RIGHT_4=1691,21
PRIMER_LEFT_4_TM=59.752
PRIMER_RIGHT_4_TM=60.929
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_05499 Ps_contig_mira-and-tgicl-ass_franssen_38146
SEQUENCE=TAGTTTTTTTCTACTGTTAATCTCTCTCTCTCCCAAACAGGTTTCACAGAAGGTTCATTTTTTYCCACTTGCAAATTCTAAAAGTGCTCTATAGAATTTTGATGCACCAAAACCATCACAAACGCTATGTGACAATCCCATACTAATTGTGAAGCCACCACAAAGAAACTTTGTAACTTTGAAAACCAAAGGATAAGGACTAGTTTGGTCTTGTGAAGGGTTATCAAACACTAGTTTTTGTGCCGTTGGAACATCAATGCCTTCAAGATAGTTAAGGGAAGAGAGTTCACAGTTTGCTTTTGCTTCTAAGAATGGAACTCCATCACCATTGCAGTTGATTCCAAGCTTTTCATCATCAAGTGTTGTTATTTTTCCTGCAAGAGGGTAATAGTAAACCAAAGCCTTTGAGAGTGCTTCTTTGATTAGAGAAGAAGGGTCTTTTTGGTCACTTGGGAAATCATGATTTGCTTTGTAGACATATATGGTTTGGCAAAGAATGTTGATGTTAGGATCATTGTCAATGGTGGAGAGTGAAAGAATTTCAGAGGTGTGGGTTTGGAGGGTTTAACTATGATAACATCCTTGGTATGAAGACAAAGGAGAGAGGGTGGCTTGTGATTTTCCATGGAGATAGATATGAAGAGAACTAAGATGTC
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05500 Ps_contig_mira-and-tgicl-ass_franssen_38173
SEQUENCE=AAAGACAAATTGGAAAAGGAACTAATGATGTCCTCAAAGAACAATAATGATGCTGTTAAGTTTAGTACAATGATGCACTTCAAGCTCAAAACCAAAAACTTACAAGCAGAGATAATGGCATTGAAAAGTAGAGAACCAACTGAATCAATCAACCTAAATAAAGAAACAGAAGGATCAAGCAGCAACAGAAGTGAAAACAGTTCAGATATCAAGTTAGATATCTCAAGAACACAAGCTAGTGATAGTCCTCTATCAACACACCATACAACAACAACAAGCAGAACCTTCTTTCCATGTCCTTCTGCTAGGCCTTCAGGAGGAATTGCTCAACTTTTTCAAACTTCTTCGAGGCCGGAACAACTTCAATGCCAAAAGATTGATCAATTGGTCAAAGAAGAAAGCTTAAGCAACATGTTTTGTAGCATGGATGATCAAACTGGTTTTTGGCCATGGTTGGAGCAACAACATTTCAATTGAATCAAAGGGAGAGTTTTTTTATTTTATATTTCAATGTAGTTTACTTTTTTTCCAATGAGAAATCAAAAGGGTGTGTGTCAATCTGTTATAATATTTCATGAGTTATTAAATTATAAGCCTTGCTTTACAAAAACATTATGTTAATGTATAATTAAAATAGTATTTATATACAAGTAGTATTAATTTTAATAAAAGTAAAAAAAACTT
TARGET=265,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1400
PRIMER_LEFT_PENALTY=0.025566
PRIMER_RIGHT_PENALTY=0.114470
PRIMER_LEFT_SEQUENCE=AGAAACAGAAGGATCAAGCAGC
PRIMER_RIGHT_SEQUENCE=AGCAGAAGGACATGGAAAGAAG
PRIMER_LEFT=161,22
PRIMER_RIGHT=305,22
PRIMER_LEFT_TM=60.026
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=0.1400
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.114470
PRIMER_LEFT_1_SEQUENCE=AGAAACAGAAGGATCAAGCAGC
PRIMER_RIGHT_1_SEQUENCE=AGTTGAGCAATTCCTCCTGAAG
PRIMER_LEFT_1=161,22
PRIMER_RIGHT_1=331,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=171
PRIMER_PAIR_PENALTY_2=0.1738
PRIMER_LEFT_2_PENALTY=0.059316
PRIMER_RIGHT_2_PENALTY=0.114470
PRIMER_LEFT_2_SEQUENCE=ATCAAGCAGCAACAGAAGTGAA
PRIMER_RIGHT_2_SEQUENCE=AGCAGAAGGACATGGAAAGAAG
PRIMER_LEFT_2=173,22
PRIMER_RIGHT_2=305,22
PRIMER_LEFT_2_TM=60.059
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=133
PRIMER_PAIR_PENALTY_3=0.2102
PRIMER_LEFT_3_PENALTY=0.095684
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=AGCAGCAACAGAAGTGAAAACA
PRIMER_RIGHT_3_SEQUENCE=AGCAGAAGGACATGGAAAGAAG
PRIMER_LEFT_3=177,22
PRIMER_RIGHT_3=305,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.2102
PRIMER_LEFT_4_PENALTY=0.095684
PRIMER_RIGHT_4_PENALTY=0.114470
PRIMER_LEFT_4_SEQUENCE=GCAGCAACAGAAGTGAAAACAG
PRIMER_RIGHT_4_SEQUENCE=AGCAGAAGGACATGGAAAGAAG
PRIMER_LEFT_4=178,22
PRIMER_RIGHT_4=305,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=59.886
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_05501 Ps_contig_mira-and-tgicl-ass_franssen_38181
SEQUENCE=GGTTCGTTCCTACGAAAGGACGAGTAGGTAGTTCAACGACACGAGTACGCGGGACTCTCTCTTTCTCTCTCTGTCTCTATCTCTCTCTTTCTTTCTCTCACATCATTTCACAAATATTTTTCTTCTGTTTTTCTATCTAAGAAGCTGCAAGAATGTGTGGTGGTGCTATCATCTCAGATTTCATTGGTGTCAAGCGTGACCGTGACCTCTGGTTTGAGCTTGATCCTTCTGTTGATCTACTTGGTCTCGGTGCTGTTACTAACACCCCCACTTCCAAAGATTTACCTCCCCTTCACTTTCCGCAAATTTTCTCACCCAAAAGAGTGGTTGCATGTGACACTGCAGAGAAGAAACAAAGCGTGGTGACTGTAGAGAAAGGTAAGAAAAGCACCGGAGGGAAAAGAACCCGTAAGAATGTTTACAGAGGAATCAGGCAGAGGCCATGGGGGAAATGGGCTGCAGAAATAAGGGACCCACAGCAAGGTGTCCGTGTCTGGCTCGGTACTTTCTCGACGGCTGAAGAGGCCGCCAGAGCTTATGATGCTGCCGCGAAGAGAATCCGCGGAGACAAGGCTAAGCTGAATTTCCCAGATGCTCCGGTTGGTTATGCTGCTAAAGCTGTCACCGCTCCTCCAATGAAGAAGCGGTGTGTTAGCTATAGTTCTGATCAAGTCAGTTCTCAGCAGGCTAGCTCTGAGTCTGCTGGGTCTGAGATTGATCAGGAGATGGAAATCAAAAAGAAAATGTCTGATCTTGAATGGATCCTAGGTTTGGAGAATGACTTTTCTCAGGTTCAGGCCCATACTCAGGCTCAAGTTGTGCCCCAACCTCAGCCTGAGCCTGTACCCGCGCCTGCGCCATTCACTATGCCTGCTGAATGGGACAACAATTTCTTGGACGCATGGACCTTTGAAGAAATGATTGACCCCATCCATAACCTGATTTTTAACTAGGGAGTTATTAACAATGAAAAACTATTGCACAAATGGAACACCTTTGGGTGGTTCATTATTATTATTATTATTATTATTATCTTGTTTATTTACTATGTTTATGTTTAAAGTTTTGTGAACTGTATAAACTATGTGGTTTGCGATTTTAGTATGGTGTTGTCTTTGTTTCTGTCTTGGATGTGGAATGATTAAAATTATAAATCATTTAGGTGCGGCTTTTTGTAGTTGTGAATATGATTATTATTCATAAAATAAAAGTATGTAAGGTTT
TARGET=1008,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1941
PRIMER_LEFT_PENALTY=0.185822
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=CTTTGAAGAAATGATTGACCCC
PRIMER_RIGHT_SEQUENCE=CCACATCCAAGACAGAAACAAA
PRIMER_LEFT=907,22
PRIMER_RIGHT=1135,22
PRIMER_LEFT_TM=59.814
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=10.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.1941
PRIMER_LEFT_1_PENALTY=0.185822
PRIMER_RIGHT_1_PENALTY=0.008257
PRIMER_LEFT_1_SEQUENCE=CCTTTGAAGAAATGATTGACCC
PRIMER_RIGHT_1_SEQUENCE=CCACATCCAAGACAGAAACAAA
PRIMER_LEFT_1=906,22
PRIMER_RIGHT_1=1135,22
PRIMER_LEFT_1_TM=59.814
PRIMER_RIGHT_1_TM=60.008
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.2151
PRIMER_LEFT_2_PENALTY=0.185822
PRIMER_RIGHT_2_PENALTY=0.029253
PRIMER_LEFT_2_SEQUENCE=CTTTGAAGAAATGATTGACCCC
PRIMER_RIGHT_2_SEQUENCE=CATTCCACATCCAAGACAGAAA
PRIMER_LEFT_2=907,22
PRIMER_RIGHT_2=1139,22
PRIMER_LEFT_2_TM=59.814
PRIMER_RIGHT_2_TM=59.971
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=233
PRIMER_PAIR_PENALTY_3=0.2151
PRIMER_LEFT_3_PENALTY=0.185822
PRIMER_RIGHT_3_PENALTY=0.029253
PRIMER_LEFT_3_SEQUENCE=CCTTTGAAGAAATGATTGACCC
PRIMER_RIGHT_3_SEQUENCE=CATTCCACATCCAAGACAGAAA
PRIMER_LEFT_3=906,22
PRIMER_RIGHT_3=1139,22
PRIMER_LEFT_3_TM=59.814
PRIMER_RIGHT_3_TM=59.971
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.2252
PRIMER_LEFT_4_PENALTY=0.216941
PRIMER_RIGHT_4_PENALTY=0.008257
PRIMER_LEFT_4_SEQUENCE=AATGATTGACCCCATCCATAAC
PRIMER_RIGHT_4_SEQUENCE=CCACATCCAAGACAGAAACAAA
PRIMER_LEFT_4=916,22
PRIMER_RIGHT_4=1135,22
PRIMER_LEFT_4_TM=59.783
PRIMER_RIGHT_4_TM=60.008
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_05502 Ps_contig_mira-and-tgicl-ass_franssen_38189
SEQUENCE=TAGAACGAGGACACCGCTTGGGCTTAGCGGTTAGTATAGTTGGACTCTCTGGTCTATTTACAAAGCACAAAAGAGGGGCCCAAGACACAAAACCGCGGCGCCAAAAGATTTCCAGAAGTCTGCAAAAACCAGAACGTTCGTCAATGGCGGTTTTAGGCCTTTCGACTTCCACTCTCCCCTCCTTCTATCTTCGCTCTACAACCATTCACCGATCCAACCCTTCCACTATCCCTCGCTCCTTCCTCGCCCACCCTCCTTCTTTCCCTACCTTCACTCTTTCCAATCCACACTCTCAAATCGCAGTTGTCTCTTCTGATTCCAAGGCCACTTCATTTGACCTAAGTGCCCCAGATTTGGACAACAGCGGCGGCGGCGGAAGTATCAAAGGTAACGGAGACGATTTTGGCGGCGGCGGAGGCGGCGGTGAAGGAGGAGGCGGAGACGATAGTAACAAGGGAGAGGAAGGATCGAACGGAGATAAAAGGAAAATGGCTTTGTCAATGTCTCAGAAATTGACTCTAGGTTATGCTATTCTCGTCGGAGCGGGTGGTGTAATGGGCTATCTGAAGAGTGGCAGCAATAAGTCACTTTTGGCTGGTGGTTTGTCTTCTGCACTGCTGTTTTATGTTTTCACCGAACTACCCGGAAGACCTGTTCTTGCATCATCTGTGGGCCTTGGTATATCTGCTGCACTTCTTTCGGTGATGGGTTCTCGTTTCAAGAAATCAGGGAAGGTCTTTCCAGCAGGTGTTGTGTCCCTTGTGTCACTCATAATGACAGGTGGTTACTTGCACGGAATTATGCGTAGTAGTTCGCACTAGAATGTAAATAAAATAAAACAGTGAGATATCCTGTAAACCTCGCCATCTTCGTTGTTAAACTTGTGGTTTGTACTGTAGACCTGTGGTTTATGTTTGTCATACTCAGAACATTTGAAATAAAGATGATGAATGTGAAACACGCATTGCTNACTTTTTTTTAAAAAACTTTT
TARGET=364,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0529
PRIMER_LEFT_PENALTY=0.052187
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=CAAATCGCAGTTGTCTCTTCTG
PRIMER_RIGHT_SEQUENCE=AAATCGTCTCCGTTACCTTTGA
PRIMER_LEFT=293,22
PRIMER_RIGHT=402,22
PRIMER_LEFT_TM=60.052
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=0.0893
PRIMER_LEFT_1_PENALTY=0.088604
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=CCACTCTCCCCTCCTTCTATCT
PRIMER_RIGHT_1_SEQUENCE=AAATCGTCTCCGTTACCTTTGA
PRIMER_LEFT_1=168,22
PRIMER_RIGHT_1=402,22
PRIMER_LEFT_1_TM=60.089
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.1032
PRIMER_LEFT_2_PENALTY=0.102490
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=TTCCCTACCTTCACTCTTTCCA
PRIMER_RIGHT_2_SEQUENCE=AAATCGTCTCCGTTACCTTTGA
PRIMER_LEFT_2=260,22
PRIMER_RIGHT_2=402,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=143
PRIMER_PAIR_PENALTY_3=0.1032
PRIMER_LEFT_3_PENALTY=0.102490
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=TCCCTACCTTCACTCTTTCCAA
PRIMER_RIGHT_3_SEQUENCE=AAATCGTCTCCGTTACCTTTGA
PRIMER_LEFT_3=261,22
PRIMER_RIGHT_3=402,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=142
PRIMER_PAIR_PENALTY_4=0.1165
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.115769
PRIMER_LEFT_4_SEQUENCE=AAGGCCACTTCATTTGACCTAA
PRIMER_RIGHT_4_SEQUENCE=CTCCGACGAGAATAGCATAACC
PRIMER_LEFT_4=320,22
PRIMER_RIGHT_4=542,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=60.116
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_05503 Ps_contig_mira-and-tgicl-ass_franssen_38191
SEQUENCE=ACAGGTTCAATCATAAAACCAGTTATTTTACACATCCTCTAAAAAGTGCGAGCCAAAACAATTGGAATCGGAAAACCCGCCGCTCACTTCCCTCAGTCGCAAAAATCAGTCACCCCGCCGCTCCATCGCCAACAAACCGCCGCTTGTAGCCGTACGCCGCCTCAGTTAACTGTTGCAGACGCCGCCTCAGTTAACTGTTGCAAACGCCGCCGCAGGGTTTCAGCGTTACAGTAATGCCTCGAAAGCCTAGAAGGCATCAACAACTCAAATCTTGGCCGATTACATTTTCCCCTTTTGCTCGTGCTCTTGCTCATACGATGCCGAAGCGTCGTTCCGAATCTGTTGAGAACCCTACCCCTTCCTCTGCTAGTAATGGATCCACTAAGCATCCTTTAGAAGAGAGATTGGAAGTGGAAGGATCCGAGTCGTCTGATGGGGAGTTTGATGGAGTTGTCCAAGCGGATTTTGCGTTTTTTGATCCTAAATCCAATGATTTCCATGGAGTTAAGACCTTGCTGCAAACTTACCTGGATGTTGAAGAATGGGATTTGAGTGGATTTGTAGATTTGATTTTGGAACAGACCACTGTAGGGACTGTTGTGAAGGTAGAGGATGATGAGGATGAAGGGGTTTTCGCCCTTGCTACCGCTCTTAATCTATGGAGATATAGGGAGCATAGATGTATTATCCAACTCAAGGAATTTCTCCTCCATAAAGTATGCCAAGAGAAGCACATTGCTGATCAGTTGAGATTACTTTTGGGAGAACAAGCACATAATGTGGGTCTCCTTGTATCACAGCGTGTGGTGAACCTTCCTCCTCAGCTTTTACCACATCTCTATGATTCTCTTTTTAATGAAGTGTCATGGGCCACGGAAGATGAGCCAACAGAGGACCTCCGAAATTCCTTCCGGTTTAAGCATTATGTAATACTAAGCAAAATTTACAAGCACAAGAATGCAGAACAAAAAAGAAAACAGAGCGATGATAGCGATGAGGCGATAATATATGTTAAGCCTGAGGATGAAATTTTTCACAAGTTAAGCTCGTGGTCCTTCTGTTTTCCTTTACGCACCCAGCAGCCTGCACCTCACGAGCTAAAGAGTTACAGATCAATGGGATTAATCATGGCTGTTGAGGCAGACAAAATTCCAGCATTTCGACAAGAATTAGGTTCTTTAATAAATGAAAGCTAAAGGCAACTATAAGGTGTTTGAGATGCCAAGTTTTGGGATTTTTTTAATTTATTTTTTGTGGTCTTTTTTGTTTTGCAATGACAATTGACAAGATTTTGAAAATCACCTTTCCCTGTCATTACTTTCTACGTTTTAAGTTTCTCAATTGTTAGATCCTTATGAAACATGCAAAATAGTTTATACTTTGATGAAAGTGGTTGAACTTTAGAAATTGTAAAACATTTGTAACTTATTTATTTATAAAGTAGTAGTAGCAGTGTCANTTAAC
TARGET=1426,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05504 Ps_contig_mira-and-tgicl-ass_franssen_38193
SEQUENCE=AGGCCCAACCACATTGTTCAATTCTGTTCACTACCCTCCCTCCCTCCGGTTCCTTTCTNTCTTGTCGTCGCCGCTACAACTGCTACCTCCCGAATCTCCGCAAATTCTCTTCGTTCAATTTAACCTTTCAACAATTCGAAATCCTTCTGTTACTCCTATTACAAGTTTGAATAAACAAACATGCCTGACACTAGGGTAAATCCGACTTTCAGCAAGTCAACTGGTTTGCCGCGGAAGCGGTTCTATAGAGCACGAGCTCATAGCAACCCATTAAGCGACTCTCACTTCCCTGTGCCGTTAACACCCGCTCATGTTGACTATTCTCTCCATTATCCTCAGTTCTTTCCCTCATCTGATCAAGTTGATAGGTCCAAAAAGATCAAGTTTGCTGATATTGGATGTGGTTTTGGAGGGCTGCTCATTAGTCTCTCGACTCTGTTCCCGGACACACTGATGATTGGAATGGAACTTAGAGATAAAGTGTCCGAGTATGTCAAGGAACGGATTTTATCCTTAAGGGTAGCAAATCCAGGTCAATACCAAAATATTTCTGTGGTGCGAACTAACTCCATGAAGTACATTCCAAATCACTTTGACAAAGCACAACTCTCGAAGATGTTTTTCCTGTTTCCCGATCCTCATTTTAAAGAGAAGAACCATCGTCGTCGGGTTATCAGCCCATTCTTACTAGATGAGTATGCTTATGTTCTTGAGGTTGGTGGAATTATATACACGATAACAGACGTGGAAGAGCTTGGGGAATGGATGAAATCCTGTTTGGAGAATCACCCTTTGTTCGAACCGTTGACTGAGAAAGAACTTGAAGCTGATCCAGCTGTTAAGCTTTTAAGCTCTGCTACTGAAGAAGGCCAAAAGGTTGCTAGAAATGATGGACAAACTTTCCAAGCTATTTTCAGACGCATTGCACGACCTGATCAGATATCCTAGTCGCTGGTTGCTTAATCTAAGGTACTGGGAATGCAAAGAATTATTGCTACTGAAGAATCATAATCATATGGTCTTTGCGTTTTTCATTTGTTATATATATGTACTTCTTTTCATTTCATGTTGAGTAGACTTATTCGATTGAAAGCAGAATTATCTTGCAGAGTATACTTCAGAATGTAACATGTTCGGTTTGTCACTGGCGACAGTTTTGGTTCATTAGTATTTGTCTTCAATTTGATTATTCACTTTTGCAAAGTTAACACTATGAAGTTCCCTGGTTAAGGAAAATCCAATCCGAATGTAAACAAATCTTAGAGAAGTTCCCTGGTTATTCTATTTATTGCTTATACCTTTTNNANNNGCTTGGCTTCTGTTATCATCACCACACTATTTCCTATGGCTTGCATTA
TARGET=34,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1247
PRIMER_LEFT_PENALTY=0.995624
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=CCCAACCACATTGTTCAATTCT
PRIMER_RIGHT_SEQUENCE=CTTGCTGAAAGTCGGATTTACC
PRIMER_LEFT=3,22
PRIMER_RIGHT=215,22
PRIMER_LEFT_TM=60.996
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=1.2101
PRIMER_LEFT_1_PENALTY=1.081037
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=CCCAACCACATTGTTCAATTC
PRIMER_RIGHT_1_SEQUENCE=CTTGCTGAAAGTCGGATTTACC
PRIMER_LEFT_1=3,21
PRIMER_RIGHT_1=215,22
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=1.2576
PRIMER_LEFT_2_PENALTY=0.995624
PRIMER_RIGHT_2_PENALTY=0.261998
PRIMER_LEFT_2_SEQUENCE=CCCAACCACATTGTTCAATTCT
PRIMER_RIGHT_2_SEQUENCE=GGAAGTGAGAGTCGCTTAATGG
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=288,22
PRIMER_LEFT_2_TM=60.996
PRIMER_RIGHT_2_TM=60.262
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=1.3135
PRIMER_LEFT_3_PENALTY=0.995624
PRIMER_RIGHT_3_PENALTY=0.317850
PRIMER_LEFT_3_SEQUENCE=CCCAACCACATTGTTCAATTCT
PRIMER_RIGHT_3_SEQUENCE=CAAACCAGTTGACTTGCTGAAA
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=227,22
PRIMER_LEFT_3_TM=60.996
PRIMER_RIGHT_3_TM=60.318
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=1.3395
PRIMER_LEFT_4_PENALTY=0.995624
PRIMER_RIGHT_4_PENALTY=0.343828
PRIMER_LEFT_4_SEQUENCE=CCCAACCACATTGTTCAATTCT
PRIMER_RIGHT_4_SEQUENCE=GAGTCGCTTAATGGGTTGCTAT
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=280,22
PRIMER_LEFT_4_TM=60.996
PRIMER_RIGHT_4_TM=59.656
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_05505 Ps_contig_mira-and-tgicl-ass_franssen_38231
SEQUENCE=GGTATCAACGCAGAGTGCGCGGGGAAATAAAAGAAAGTGAATATCTGAATGAAGGAAGGAGAGCAGAGAAAGATAATGTTAATTTGTGGGCCGAGTTTATTGCCTTGCCTACGCGTTGGGGGTTTTGAAAATTGCAAAGCGAAGAAGAAGAAGAAGAACAAGTGGAGTATGAGTTGCAAATCCCAGAGCGATTGTGTGAGTAGAAAATGGCGCAATTACAGTGTGAGACTTGTTACCTTCCTGGCAGCTGCTACGGTAATAGGTTCAATTGCAACAGGCGATGCAATTTACGAACAGGAAGAGACTCTGTCAAACATACCTCAAACTCTATCGGGTGAAGGTAAGAAAGCTAAAATACAGAAACCCAAGTCGAGGAAAGCGGAATCCTGCACGGTTAAGTGTGTTACCACTTGCATCAGAGGCGGCGAAGGCTCTCCTGGTGAAGGCCCTCTTAACGTCACAAGACCACTCGTTGTTTTCAAGCAAGGCTTTCGGACTCGTCACTACTGTTTGGTGGAGTGTTCGGATATTTGTAATTTGATGACCAACGCTGACGACATGCCTTGAATTTATATTTATATCCATTACCGATACTAATAAACGTTATATAATGTAACTAAGCTTCGCTTATTTTACTTTTTTNAANTATTTTTTACATTTCTTTTCTATCATTTTATACATTTTTCTTATAATATTACATATTTAACTA
TARGET=141,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2345
PRIMER_LEFT_PENALTY=0.232153
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=GTTAATTTGTGGGCCGAGTTTA
PRIMER_RIGHT_SEQUENCE=TTCACCCGATAGAGTTTGAGGT
PRIMER_LEFT=77,22
PRIMER_RIGHT=338,22
PRIMER_LEFT_TM=60.232
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=0.2345
PRIMER_LEFT_1_PENALTY=0.232153
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=GTTAATTTGTGGGCCGAGTTTA
PRIMER_RIGHT_1_SEQUENCE=ACCTTCACCCGATAGAGTTTGA
PRIMER_LEFT_1=77,22
PRIMER_RIGHT_1=341,22
PRIMER_LEFT_1_TM=60.232
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.2345
PRIMER_LEFT_2_PENALTY=0.232153
PRIMER_RIGHT_2_PENALTY=0.002310
PRIMER_LEFT_2_SEQUENCE=GTTAATTTGTGGGCCGAGTTTA
PRIMER_RIGHT_2_SEQUENCE=TCCTCGACTTGGGTTTCTGTAT
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=375,22
PRIMER_LEFT_2_TM=60.232
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=0.3554
PRIMER_LEFT_3_PENALTY=0.232153
PRIMER_RIGHT_3_PENALTY=0.123278
PRIMER_LEFT_3_SEQUENCE=GTTAATTTGTGGGCCGAGTTTA
PRIMER_RIGHT_3_SEQUENCE=CCTTCACCCGATAGAGTTTGAG
PRIMER_LEFT_3=77,22
PRIMER_RIGHT_3=340,22
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=60.123
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=0.3554
PRIMER_LEFT_4_PENALTY=0.232153
PRIMER_RIGHT_4_PENALTY=0.123278
PRIMER_LEFT_4_SEQUENCE=GTTAATTTGTGGGCCGAGTTTA
PRIMER_RIGHT_4_SEQUENCE=CTTCACCCGATAGAGTTTGAGG
PRIMER_LEFT_4=77,22
PRIMER_RIGHT_4=339,22
PRIMER_LEFT_4_TM=60.232
PRIMER_RIGHT_4_TM=60.123
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_05506 Ps_contig_mira-and-tgicl-ass_franssen_38234
SEQUENCE=TTTTAGTTTTTGTTGCATTGCAAGTACCATGTTGAGAAAATATGGATTCAAACAACTATGACTAACTACAAAGCTAATCTCATACTTACATCACATGCATCTATACTATCAAGCATAACTACAACAAGTACCATTTTTGTTATGATGAATAACAAAGGCAGAATGGGAGGCTAAATTACCTACTTATTTGACATTTAAGCTTTTACTGCACATTGCTTTGTAATAACACCATTTTAAGTGCACCAATTTTTGTCTCTAAATCTGAAACCATATATATTAGTACCTATGGGTCTTCTTGCTTTGGCTTCTGGCTCAATTCTGAGTAATCACCACCCATCAGTTGCAAAATCTCCTCCCACATACCTTCAGAAGAATCTGTCATAGCCTCATACAGCAAACTTGAGCTACATGCAGCAACATGCCAATACTCGGACTCAATCTCATCTCTCAACTGATCTATTTGCCATCCAGCATAACCAATAAAGAAACTGAAATCACGAGGCTTGATAATCCCTTTCTTCACAAGCCTTGATGCATCATCCAAACTGTTTCGAGCTCCAAAATATAGTCCAGGGACTACCTCTTCAAATCCTGGAACCTTCAACTTCTCCCCTGATTTTAGCAAAAACATGCTTGCCTCAAGAGGTCCACCAAAATGCAAAGAACAGTCAGAAAAGGAGGTTGTTAAATCCTGATTTGTAGGTCTTATGTTTTTTATCTTTTTGTGAAGAGGACGGTTGATGACAATTCCAAAAGGCCCTTCTTGTGACTGTCTAGTTCCAGATCTGAGAAGGAGAACAACAGTTCTTTCAAATGTGCGAACACCATCAAGCTTTTCGGTAGCCACAAGTACACAGCCAGTCTCAGGTCCAGGAATTGGATGAGCCCACTTTGTTCCAAGAGGTTTAGAATTGTCTATTGTCCCACCTTCTTTTTGTGTGTCGGTATCTTCTATTTCTTTCAGCTCATCTCTATATAGTTTTGCTCTGAAGTCACGCCAATCCAGATTAATCTGATGACGCTTCTGAGAGTTGTTATTTGATTTATCACCATCATTATTTCCTGGAATAGACGGTTCACCATTACCGGAAGAAGAAGAAGAAGAGTTGCCGTGATTGTTGTTGTTGGCGGAAGCAGTGAGAAGAAAAGGGCGATAGTGTTGTTTAGTGAAAAGTGTGATCTCGGAAAATGAATTACAAGACCTTGTTCCAGAAATGGGTCTGTAGCAAGATGAATTTGCGGGGAAAAGGTTCTTCACACACAGATCCATTGCTATNACCGARYTAAAGAGGGTAAGATG
TARGET=1089,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05507 Ps_contig_mira-and-tgicl-ass_franssen_38234
SEQUENCE=TTTTAGTTTTTGTTGCATTGCAAGTACCATGTTGAGAAAATATGGATTCAAACAACTATGACTAACTACAAAGCTAATCTCATACTTACATCACATGCATCTATACTATCAAGCATAACTACAACAAGTACCATTTTTGTTATGATGAATAACAAAGGCAGAATGGGAGGCTAAATTACCTACTTATTTGACATTTAAGCTTTTACTGCACATTGCTTTGTAATAACACCATTTTAAGTGCACCAATTTTTGTCTCTAAATCTGAAACCATATATATTAGTACCTATGGGTCTTCTTGCTTTGGCTTCTGGCTCAATTCTGAGTAATCACCACCCATCAGTTGCAAAATCTCCTCCCACATACCTTCAGAAGAATCTGTCATAGCCTCATACAGCAAACTTGAGCTACATGCAGCAACATGCCAATACTCGGACTCAATCTCATCTCTCAACTGATCTATTTGCCATCCAGCATAACCAATAAAGAAACTGAAATCACGAGGCTTGATAATCCCTTTCTTCACAAGCCTTGATGCATCATCCAAACTGTTTCGAGCTCCAAAATATAGTCCAGGGACTACCTCTTCAAATCCTGGAACCTTCAACTTCTCCCCTGATTTTAGCAAAAACATGCTTGCCTCAAGAGGTCCACCAAAATGCAAAGAACAGTCAGAAAAGGAGGTTGTTAAATCCTGATTTGTAGGTCTTATGTTTTTTATCTTTTTGTGAAGAGGACGGTTGATGACAATTCCAAAAGGCCCTTCTTGTGACTGTCTAGTTCCAGATCTGAGAAGGAGAACAACAGTTCTTTCAAATGTGCGAACACCATCAAGCTTTTCGGTAGCCACAAGTACACAGCCAGTCTCAGGTCCAGGAATTGGATGAGCCCACTTTGTTCCAAGAGGTTTAGAATTGTCTATTGTCCCACCTTCTTTTTGTGTGTCGGTATCTTCTATTTCTTTCAGCTCATCTCTATATAGTTTTGCTCTGAAGTCACGCCAATCCAGATTAATCTGATGACGCTTCTGAGAGTTGTTATTTGATTTATCACCATCATTATTTCCTGGAATAGACGGTTCACCATTACCGGAAGAAGAAGAAGAAGAGTTGCCGTGATTGTTGTTGTTGGCGGAAGCAGTGAGAAGAAAAGGGCGATAGTGTTGTTTAGTGAAAAGTGTGATCTCGGAAAATGAATTACAAGACCTTGTTCCAGAAATGGGTCTGTAGCAAGATGAATTTGCGGGGAAAAGGTTCTTCACACACAGATCCATTGCTATNACCGARYTAAAGAGGGTAAGATG
TARGET=1116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05508 Ps_contig_mira-and-tgicl-ass_franssen_38239
SEQUENCE=TACTTTGTCAGGTAAGCAAACAGGAAGTGAGTCGTCCCTCAGCATATCTTCGTGGTCATCATCTTCATCATCAGAAATATCATCATCATCTTCGATAACCAAAGCCTCATCAATTTCAACAGGAGATATATCTAAATCTTCATCCTCAGAAGTAGAATCCTCATTTGCAGGCTTCATTTGAACATCCTTATCAACAGCTAGACTCTTCTGCGTGCAGCATTTCT
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1452
PRIMER_LEFT_PENALTY=0.103187
PRIMER_RIGHT_PENALTY=0.041981
PRIMER_LEFT_SEQUENCE=TCAGCATATCTTCGTGGTCATC
PRIMER_RIGHT_SEQUENCE=CGCAGAAGAGTCTAGCTGTTGA
PRIMER_LEFT=38,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=60.103
PRIMER_RIGHT_TM=59.958
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=0.2572
PRIMER_LEFT_1_PENALTY=0.215215
PRIMER_RIGHT_1_PENALTY=0.041981
PRIMER_LEFT_1_SEQUENCE=TTGTCAGGTAAGCAAACAGGAA
PRIMER_RIGHT_1_SEQUENCE=CGCAGAAGAGTCTAGCTGTTGA
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=211,22
PRIMER_LEFT_1_TM=59.785
PRIMER_RIGHT_1_TM=59.958
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=0.2572
PRIMER_LEFT_2_PENALTY=0.215215
PRIMER_RIGHT_2_PENALTY=0.041981
PRIMER_LEFT_2_SEQUENCE=TTTGTCAGGTAAGCAAACAGGA
PRIMER_RIGHT_2_SEQUENCE=CGCAGAAGAGTCTAGCTGTTGA
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=59.785
PRIMER_RIGHT_2_TM=59.958
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=0.3013
PRIMER_LEFT_3_PENALTY=0.259289
PRIMER_RIGHT_3_PENALTY=0.041981
PRIMER_LEFT_3_SEQUENCE=GAGTCGTCCCTCAGCATATCTT
PRIMER_RIGHT_3_SEQUENCE=CGCAGAAGAGTCTAGCTGTTGA
PRIMER_LEFT_3=28,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=59.741
PRIMER_RIGHT_3_TM=59.958
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=0.3272
PRIMER_LEFT_4_PENALTY=0.103187
PRIMER_RIGHT_4_PENALTY=0.224014
PRIMER_LEFT_4_SEQUENCE=TCAGCATATCTTCGTGGTCATC
PRIMER_RIGHT_4_SEQUENCE=AAGCCTGCAAATGAGGATTCTA
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=173,22
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=60.224
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=136
=
PRIMER_SEQUENCE_ID=SSR_05509 Ps_contig_mira-and-tgicl-ass_franssen_38244
SEQUENCE=CAACAAGTTTGGCTATCATATCTGGAATGCTGGTCACAACTAGTCTCATGTCGCTAGTCATCGCTTTGTATTGGGAGAAGAATTTGTTGATATCTGCATGCTTTCTGTTATGTTTTGGCGTTATCGAGGTTGCATATCTTTCGGCCTGTATGCTGCAATTTCACAGGGGAGCTTGGTATCTAGTTGTCCTTTTGATAGTGTCCATGACAGTCATGCTTTCATGGCATTATGGAACGGTGAAGAAGTATGAGTTTGATTTACAGAACAAAGTATCAACTGAATGGCTAATAGATGTTAGTCCGGGTCTCGGTATTTCAAGGGTAGCTGGAATTGGCTTCATTTACACAGACATAGTGACAGGCATCCCAGCTTTCTTTTCACATTTTATTACTAATCTTCCTGCATTTCATCAAGTGTTGATCATGGTGTCCTTCAAGTCTATGCCGGTGCCTCATGTTCCCGAAAGTGAAAGGTATCTCATAGGAAGGATTGGACCAAGAGATTATAAAATATATCGTTGCATTGTGAGATATGGATATTGTGATAATATAAGGGACACAAATGATTTTGAGGAGAAGATCATTCGTGCTATTGGAGAATTCATTTCCATCGAACATGTTACTGACATTGAATCAATGGTTTCGCCAGATGAAAGAATGATTATAGTAGGAAATTCATCCGACGGAAATGCATTAGTCCCTCTGATGAATGAAACAAGCAACCAAGTGAGCATTATGAATGAAGCTCAGGTAAGTCCAATAGCAATGGATGATAATGAGCATACGGTATTGGCGAGTAGTAGTAAGTCTTGTAAGAAAAGAAAAAAAGTGAGGTTCATGTTGCCACCTGCTAGTAGTGTTCCTAAAATGGATGAATCTGTTAAGAAGGAGCTTTTGGAACTGATTGATGCAAGGGAGAGTGGAAGTGCTTATTTCTTAGGACAGTCACATTTGGTGGTGCGTGGTGGATCGAACTATCTAAAGATATTCTTAATAATGGTATTTCGTTTCTTTGAAAGAAATGGGAGGGAACCACCAGTTGCTCTGAAAATCCCTCATGCTGCTCTAGTAGAAGTTGGTATGATATGTACTATATGATAGGAGGAGAGATCAAAGTTGATACATACATATAATAGATAGATAGAATCAAGAGATGTATAAAAACAATATAATTCCAAGTGTTTTTCTATTTCTAAACTTAATTGCAGGTCCCCCATTATCCAAGATTTCCTATAATGCCTAATTTTAANGGCATGTCTCAACCAAACTCAAAGAAAC
TARGET=1132,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1939
PRIMER_LEFT_PENALTY=0.039879
PRIMER_RIGHT_PENALTY=0.154023
PRIMER_LEFT_SEQUENCE=AACCACCAGTTGCTCTGAAAAT
PRIMER_RIGHT_SEQUENCE=TTTGAGTTTGGTTGAGACATGC
PRIMER_LEFT=1029,22
PRIMER_RIGHT=1271,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=60.154
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.6139
PRIMER_LEFT_1_PENALTY=0.039879
PRIMER_RIGHT_1_PENALTY=0.574044
PRIMER_LEFT_1_SEQUENCE=AACCACCAGTTGCTCTGAAAAT
PRIMER_RIGHT_1_SEQUENCE=GTTTGGTTGAGACATGCCNTTA
PRIMER_LEFT_1=1029,22
PRIMER_RIGHT_1=1266,22
PRIMER_LEFT_1_TM=60.040
PRIMER_RIGHT_1_TM=60.574
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.9250
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.6910
PRIMER_LEFT_2_PENALTY=0.536975
PRIMER_RIGHT_2_PENALTY=0.154023
PRIMER_LEFT_2_SEQUENCE=ACCACCAGTTGCTCTGAAAATC
PRIMER_RIGHT_2_SEQUENCE=TTTGAGTTTGGTTGAGACATGC
PRIMER_LEFT_2=1030,22
PRIMER_RIGHT_2=1271,22
PRIMER_LEFT_2_TM=60.537
PRIMER_RIGHT_2_TM=60.154
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.7565
PRIMER_LEFT_3_PENALTY=0.602508
PRIMER_RIGHT_3_PENALTY=0.154023
PRIMER_LEFT_3_SEQUENCE=AAATCCCTCATGCTGCTCTAGT
PRIMER_RIGHT_3_SEQUENCE=TTTGAGTTTGGTTGAGACATGC
PRIMER_LEFT_3=1047,22
PRIMER_RIGHT_3=1271,22
PRIMER_LEFT_3_TM=59.397
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=0.7638
PRIMER_LEFT_4_PENALTY=0.039879
PRIMER_RIGHT_4_PENALTY=0.723886
PRIMER_LEFT_4_SEQUENCE=AACCACCAGTTGCTCTGAAAAT
PRIMER_RIGHT_4_SEQUENCE=GAAATCTTGGATAATGGGGGAC
PRIMER_LEFT_4=1029,22
PRIMER_RIGHT_4=1228,22
PRIMER_LEFT_4_TM=60.040
PRIMER_RIGHT_4_TM=60.724
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_05510 Ps_contig_mira-and-tgicl-ass_franssen_38245
SEQUENCE=CGTCTTTTGTAAAATTTTTGTTGGCTCTCCAAATGGAAAAAAATAGCCAGCATTATTAATGAGAACGTGCAAGGGTGCTGCGCGGGCATTCCACGCTTCAGCAAACCTAGCAACCGAATCCAATGAGAGAAGATCCACCACCATCACCTCAACATTGAGTGCAATACCCAATCCAGCAGATTCAATCTGCCATTTCTGGATTAACTCGGTAGCCACCTTCGCATTCCTCACGGCCATAACAACATGTGCCCCTGATTGTGCCAATTGCCTAGCAATTTCAAGACCAATGCCACTAGTGGAACCAGTAACAATACAAGTGAGTTCATTGATTGGTGGTAATGGCATAGGGTTCTCCAAGTGACTCGCCATGATTCGCTGGAATAGAAATTCGTAGATTATATTGAACCATCCTCTAAACCATTCTATCCAACCTAAACACTCTTTCTTTTTCTTCTTCAATGGTTCAACGCTTGAAGACGACGACGCCGTTTTTGATGCCATCTTCTTTCTCTGCTGTGAGTGAGTGAGAGTATAGAGAATCTGAGATCTATAATGGTGGATTGATGGGTTACAGTGTGAGTTTTTGAGATTCGATGAGTTGACTCGGGTGAGTTAACTCGTTCAGTGAATTTACAATGGACACACCTAACAACACATAGTTAGGGGCCNNNTTTTCTTTCTTAGAACTG
TARGET=516,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3451
PRIMER_LEFT_PENALTY=0.062198
PRIMER_RIGHT_PENALTY=0.282868
PRIMER_LEFT_SEQUENCE=GATTCGCTGGAATAGAAATTCG
PRIMER_RIGHT_SEQUENCE=TCACACTGTAACCCATCAATCC
PRIMER_LEFT=369,22
PRIMER_RIGHT=578,22
PRIMER_LEFT_TM=60.062
PRIMER_RIGHT_TM=59.717
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=210
PRIMER_PAIR_PENALTY_1=0.4072
PRIMER_LEFT_1_PENALTY=0.010294
PRIMER_RIGHT_1_PENALTY=0.396944
PRIMER_LEFT_1_SEQUENCE=GATGCCATCTTCTTTCTCTGCT
PRIMER_RIGHT_1_SEQUENCE=CGAGTCAACTCATCGAATCTCA
PRIMER_LEFT_1=493,22
PRIMER_RIGHT_1=605,22
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=60.397
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=0.4868
PRIMER_LEFT_2_PENALTY=0.203971
PRIMER_RIGHT_2_PENALTY=0.282868
PRIMER_LEFT_2_SEQUENCE=GTAATGGCATAGGGTTCTCCAA
PRIMER_RIGHT_2_SEQUENCE=TCACACTGTAACCCATCAATCC
PRIMER_LEFT_2=335,22
PRIMER_RIGHT_2=578,22
PRIMER_LEFT_2_TM=60.204
PRIMER_RIGHT_2_TM=59.717
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.5520
PRIMER_LEFT_3_PENALTY=0.269153
PRIMER_RIGHT_3_PENALTY=0.282868
PRIMER_LEFT_3_SEQUENCE=GTGGTAATGGCATAGGGTTCTC
PRIMER_RIGHT_3_SEQUENCE=TCACACTGTAACCCATCAATCC
PRIMER_LEFT_3=332,22
PRIMER_RIGHT_3=578,22
PRIMER_LEFT_3_TM=59.731
PRIMER_RIGHT_3_TM=59.717
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.5625
PRIMER_LEFT_4_PENALTY=0.279595
PRIMER_RIGHT_4_PENALTY=0.282868
PRIMER_LEFT_4_SEQUENCE=TTGATTGGTGGTAATGGCATAG
PRIMER_RIGHT_4_SEQUENCE=TCACACTGTAACCCATCAATCC
PRIMER_LEFT_4=325,22
PRIMER_RIGHT_4=578,22
PRIMER_LEFT_4_TM=59.720
PRIMER_RIGHT_4_TM=59.717
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_05511 Ps_contig_mira-and-tgicl-ass_franssen_38255
SEQUENCE=TGCATGAAGTAGGAGAAGGTTTAGATCTTTTTTGTTTGTTTTTTYTTYATAGCTTCAAGGTAAGTCTCTGAGCAACAGTGAGGAAACGAGCTTTGGAGTGAGGTTGAGAGAGATAGCCACGAGAAAGAAGAGAGGGTGATTCTGTGTTATTGTTATTGATTGAAAAAAATAAAATCCAGTGAGGAATGCCCTAATTTATAGAGCTTAAAAACAGTTAGCAAATACGCTCAAAATTAGGCTGAAATTCACCTCAAATGCAACAGTTAGTTAGGGTTGGTTAGTAATGCAATGGTTCTGTTATTGAATGAATATGAAATAATTTTATTTGGAATTTGAGATTAATGATTTATGAGTTTAAAGTTAATTCCATTTGAATTTATAGTAAATTTCAACTAGGGTTAATGGACTGAATCGATTTCTTGCAGAATGTAATTCCACTCAATACTTCTGTTAATGCAAAAGCAGTTAGGAAAATGAGTTGGATTCAATTGCTTGCATCAATTTTGTTAGTTAGTTAGGCTTAAATGTTAAGGTGGAAAACCAGTTGAGCAACGCACGGAATTAAACTGAATCTATCAGGAATTCGACTTTGAAACTTCCTGATTTTCTTGTTAATGCAGGTTAATATATTCTGATTTGAGTTGTTTGTGGACTTTGCAGGTTGGAAAAGCAGGATTATACAAGATGGTGACYGATGTTGTAGAATTARTGCATTCGTTGGTTTTGGTCAATGCTATCATTGTGACC
TARGET=506,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05512 Ps_contig_mira-and-tgicl-ass_franssen_38256
SEQUENCE=CCTTGAGGATATATTATTAGGGATAATTAATTAGGTGAAGGGTAAATCGGGAAGAAAAAATGTCGCAGCGATCGGTGCCGGCGCCGTTTTTGACGAAGACGTATCAGATGGTGGATGATTTTGCGACGAAGGATGTGATATCATGGAATGAAAGTGGCGAAAGTTTTGTTGTTTGGAAACATGCGGATTTTGCAAGGGATTTGCTTCCTAAGTATTTTAAACACAATAATTTCTCCAGCTTTGTTCGCCAGCTCAACACATATGGATTCCGAAAAGTTGTGGCAGACAAATGGGAATTCGCAAACGAAAATTTCAAACGAGGCCAGAAAGATCTCCTCACCGAAATCAAACGCCGCAAAACCGTATCACAACCGTCGTCACATCCTCCAGAAACCGAAAAAACCGACGCCAACGGCAACAACTCTCCTTCAAACTCCGACGACATCAACGGAGTCGGATCCACCTCCACAGTCCGATCAACCTCTTCCTCCAATTCAAAAAACCCCGGATCAGTAGAAACAACACTTCACAGTGCGTCAATTTATCGTCCGAGAACGAGAAACTGAAAAAGGAAAACGAGATATTGAGTTGGGAGTTAGCACGTGCGAAGCACAAGTGCGATGAGTTGGTGTCTTTTCAAAAGACCGTTTAAACGTGGGACCCGATCAGATCGATCGCATTATGAAAGAAAGAAACTGTGAACCGGTTCAAAATGCGGTTGGTGAAGGTGAAGAAAATGGAAGAGATAGTTTGAAACTGTTTGGTGTTTGGTTGAAAGAAGAAAACTATACAGAGAAAAGAAACAATAATAAAAAAAGAATTTGTGAACAACAATTGGACTTCGGTGGGCCCAGGGCTAAAGTGACAAACAATGTTGTTGACTTTAGTGCTGTCAATGTTGTGAACATGATGAAAAGTGGCAA
TARGET=684,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3920
PRIMER_LEFT_PENALTY=0.059875
PRIMER_RIGHT_PENALTY=0.332137
PRIMER_LEFT_SEQUENCE=ATCAACCTCTTCCTCCAATTCA
PRIMER_RIGHT_SEQUENCE=TTCCATTTTCTTCACCTTCACC
PRIMER_LEFT=475,22
PRIMER_RIGHT=741,22
PRIMER_LEFT_TM=59.940
PRIMER_RIGHT_TM=60.332
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.4787
PRIMER_LEFT_1_PENALTY=0.146525
PRIMER_RIGHT_1_PENALTY=0.332137
PRIMER_LEFT_1_SEQUENCE=CGATGAGTTGGTGTCTTTTCAA
PRIMER_RIGHT_1_SEQUENCE=TTCCATTTTCTTCACCTTCACC
PRIMER_LEFT_1=618,22
PRIMER_RIGHT_1=741,22
PRIMER_LEFT_1_TM=60.147
PRIMER_RIGHT_1_TM=60.332
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.5255
PRIMER_LEFT_2_PENALTY=0.146525
PRIMER_RIGHT_2_PENALTY=0.378997
PRIMER_LEFT_2_SEQUENCE=CGATGAGTTGGTGTCTTTTCAA
PRIMER_RIGHT_2_SEQUENCE=TCATGTTCACAACATTGACAGC
PRIMER_LEFT_2=618,22
PRIMER_RIGHT_2=909,22
PRIMER_LEFT_2_TM=60.147
PRIMER_RIGHT_2_TM=59.621
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=292
PRIMER_PAIR_PENALTY_3=0.5913
PRIMER_LEFT_3_PENALTY=0.259188
PRIMER_RIGHT_3_PENALTY=0.332137
PRIMER_LEFT_3_SEQUENCE=AAACAACACTTCACAGTGCGTC
PRIMER_RIGHT_3_SEQUENCE=TTCCATTTTCTTCACCTTCACC
PRIMER_LEFT_3=516,22
PRIMER_RIGHT_3=741,22
PRIMER_LEFT_3_TM=60.259
PRIMER_RIGHT_3_TM=60.332
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=226
PRIMER_PAIR_PENALTY_4=0.5913
PRIMER_LEFT_4_PENALTY=0.259188
PRIMER_RIGHT_4_PENALTY=0.332137
PRIMER_LEFT_4_SEQUENCE=GAAACAACACTTCACAGTGCGT
PRIMER_RIGHT_4_SEQUENCE=TTCCATTTTCTTCACCTTCACC
PRIMER_LEFT_4=515,22
PRIMER_RIGHT_4=741,22
PRIMER_LEFT_4_TM=60.259
PRIMER_RIGHT_4_TM=60.332
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_05513 Ps_contig_mira-and-tgicl-ass_franssen_38302
SEQUENCE=GTTTTAAGCTTTTCTTTCTCTTTTCTTCTTTTTTTNAACCTTTTCATAAAGTAACGCTAATGGTAGCGCTTTAGATTCTTCTTCTTCTACACATTCATCATCTTTCTTCTTTTTCTCTATTCTTGTTCTTGTTGTTGTTGTAATTCTTCAACTTTACTTTCTGCAACCAAACAACTAATCAATTTGCTTAATGGAAACTGCATAGGCTTTGTTTGGTTGGCTCTATACGCCTTAGTCTTCGCTATGACAAAGATTAGTCCTGAAATTGAAACTTTGATGCCAATGGAAGCAGTTCCGCCTGTTTCGGCCGATGTGAGCTTTATTTCTAATAGTTTTCCGAAGTATAAACTAGGGGCCG
TARGET=76,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0980
PRIMER_LEFT_PENALTY=0.070333
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=CGCTAATGGTAGCGCTTTAGAT
PRIMER_RIGHT_SEQUENCE=ACAAAGCCTATGCAGTTTCCAT
PRIMER_LEFT=54,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=59.930
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=0.3984
PRIMER_LEFT_1_PENALTY=0.070333
PRIMER_RIGHT_1_PENALTY=0.328094
PRIMER_LEFT_1_SEQUENCE=CGCTAATGGTAGCGCTTTAGAT
PRIMER_RIGHT_1_SEQUENCE=AAACAAAGCCTATGCAGTTTCC
PRIMER_LEFT_1=54,22
PRIMER_RIGHT_1=213,22
PRIMER_LEFT_1_TM=59.930
PRIMER_RIGHT_1_TM=59.672
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.4391
PRIMER_LEFT_2_PENALTY=0.411344
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=ACGCTAATGGTAGCGCTTTAGA
PRIMER_RIGHT_2_SEQUENCE=ACAAAGCCTATGCAGTTTCCAT
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=60.411
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.4732
PRIMER_LEFT_3_PENALTY=0.411344
PRIMER_RIGHT_3_PENALTY=0.061818
PRIMER_LEFT_3_SEQUENCE=ACGCTAATGGTAGCGCTTTAGA
PRIMER_RIGHT_3_SEQUENCE=ACCAAACAAAGCCTATGCAGTT
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=216,22
PRIMER_LEFT_3_TM=60.411
PRIMER_RIGHT_3_TM=60.062
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.4732
PRIMER_LEFT_4_PENALTY=0.411344
PRIMER_RIGHT_4_PENALTY=0.061818
PRIMER_LEFT_4_SEQUENCE=ACGCTAATGGTAGCGCTTTAGA
PRIMER_RIGHT_4_SEQUENCE=AACCAAACAAAGCCTATGCAGT
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=60.411
PRIMER_RIGHT_4_TM=60.062
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_05514 Ps_contig_mira-and-tgicl-ass_franssen_38302
SEQUENCE=GTTTTAAGCTTTTCTTTCTCTTTTCTTCTTTTTTTNAACCTTTTCATAAAGTAACGCTAATGGTAGCGCTTTAGATTCTTCTTCTTCTACACATTCATCATCTTTCTTCTTTTTCTCTATTCTTGTTCTTGTTGTTGTTGTAATTCTTCAACTTTACTTTCTGCAACCAAACAACTAATCAATTTGCTTAATGGAAACTGCATAGGCTTTGTTTGGTTGGCTCTATACGCCTTAGTCTTCGCTATGACAAAGATTAGTCCTGAAATTGAAACTTTGATGCCAATGGAAGCAGTTCCGCCTGTTTCGGCCGATGTGAGCTTTATTTCTAATAGTTTTCCGAAGTATAAACTAGGGGCCG
TARGET=129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0980
PRIMER_LEFT_PENALTY=0.070333
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=CGCTAATGGTAGCGCTTTAGAT
PRIMER_RIGHT_SEQUENCE=ACAAAGCCTATGCAGTTTCCAT
PRIMER_LEFT=54,22
PRIMER_RIGHT=211,22
PRIMER_LEFT_TM=59.930
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=0.3984
PRIMER_LEFT_1_PENALTY=0.070333
PRIMER_RIGHT_1_PENALTY=0.328094
PRIMER_LEFT_1_SEQUENCE=CGCTAATGGTAGCGCTTTAGAT
PRIMER_RIGHT_1_SEQUENCE=AAACAAAGCCTATGCAGTTTCC
PRIMER_LEFT_1=54,22
PRIMER_RIGHT_1=213,22
PRIMER_LEFT_1_TM=59.930
PRIMER_RIGHT_1_TM=59.672
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.4391
PRIMER_LEFT_2_PENALTY=0.411344
PRIMER_RIGHT_2_PENALTY=0.027715
PRIMER_LEFT_2_SEQUENCE=ACGCTAATGGTAGCGCTTTAGA
PRIMER_RIGHT_2_SEQUENCE=ACAAAGCCTATGCAGTTTCCAT
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=60.411
PRIMER_RIGHT_2_TM=60.028
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.4732
PRIMER_LEFT_3_PENALTY=0.411344
PRIMER_RIGHT_3_PENALTY=0.061818
PRIMER_LEFT_3_SEQUENCE=ACGCTAATGGTAGCGCTTTAGA
PRIMER_RIGHT_3_SEQUENCE=ACCAAACAAAGCCTATGCAGTT
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=216,22
PRIMER_LEFT_3_TM=60.411
PRIMER_RIGHT_3_TM=60.062
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=164
PRIMER_PAIR_PENALTY_4=0.4732
PRIMER_LEFT_4_PENALTY=0.411344
PRIMER_RIGHT_4_PENALTY=0.061818
PRIMER_LEFT_4_SEQUENCE=ACGCTAATGGTAGCGCTTTAGA
PRIMER_RIGHT_4_SEQUENCE=AACCAAACAAAGCCTATGCAGT
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=60.411
PRIMER_RIGHT_4_TM=60.062
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_05515 Ps_contig_mira-and-tgicl-ass_franssen_38307
SEQUENCE=GGCGGTTGTTAATGCGAGGGCTGATGCTACCTTTATGGTGCAGAGACTGAATGCAGAGAGACATTTGTGGAATGCATGGGTAACAATCTCCGAGCTTCGGCATTCAGTCATACTTAAAAGAATAAAGCTGGTACTGCTGAGGCAAAAGTTGAAACTCACTTCCATTCTAAAGGGACAGATTTTGTATTTGGAAGAATGGGCACTTTTAGATAGAGATCACTCCAGTTCTTTACTCGGAGCAACTGAAGCTTTGAAGGCCAGTACTCTCCGTCTCCCGCTTGTTGAAAAAGCAACAGCCGATGTACCCAATCTGAAAGATGCTTTGGGGTCCGCAGTTGATGTGATGCAGGCAATGGCGTCCTCAATTTATTCTCTTTCGTCAAAGGTGGAGGAAACTAACTGCTTGGTAGCGGAAATTCTAAAAGTAACCTCGAAGGAAAGGTATTTGCTTCAACATTGCAAGGATTTTTTGTCGTCCTTAGCAGCCATGCAGGTGAAGGATTGTAGCTTAAGAACGCATATGTTACAACTTTCTCGCGTACCATCAGCTTCCTGACAACAAACAAACACGTGTGTAGAACTGATC
TARGET=558,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05516 Ps_contig_mira-and-tgicl-ass_franssen_38312
SEQUENCE=TAATCAAGTAACCAATCTCAAACTACAAATTAATTAACCCTCAAACCACCAAAAAAACTCTACAAATTTTYATCAATGGCTTCCACAAACAAGGACCAAAAAACCACAAACACCGTGTCTTCTTCTTCTCCAATCTTCATTTCCACTGAGAATTTACGAACCATCCTCACCCATCAAACTCTAATGAACCACATCGACTCCAATCTCCCCAAAGTTTCAACCTTTCTCCAAACCCCAATTCGCCAAACCTACAGTCTCTCTCCTTCCTCTTCCCTCCTCCTCATGCCTTCATGGTCTTCTTCTTCCTCCTTCCCTTACGTTGGTGTCAAACTTGTAACCCATTTTCCTCAAAATTCCTCAATCAATTTACCCGGTGTTCAAGGTAGCCATGTCCTCTTCAATTCATCCACTGGTCAAACCCTTGCTTCTATGGATTCCACCGAACTTACGCTTTACAGAACCTCTTGTGTCTCTGGTTTGGCTTCTAGATATTTATCTAGAGGTGATAGTGAGATTCTTGTTATGGATGGTGCTGGTTCCCTTGCACCAC
TARGET=118,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05517 Ps_contig_mira-and-tgicl-ass_franssen_38334
SEQUENCE=AGAAACAACAAATTCACACGCATTGCTTTCTAACTTGTAAAAATGGAAAAATCAATTGAGCAAAACAAAATAGGGGAAGAAACAAACAGCGGGGTGGCGACATGTTTTCCGGCGGAAAACCAATTGATTCTATGGCGGCGGGAGGTGTCAAGGCGCGGCGGTTGTTGTTGGCTGGAAAACACGATTCTGGCGGATGGAGGAAACGGTAGATGGAGGTGTTGTAGCCGTGATTGACATGAGATGTTGTTCTTCAATTGCATGCGGCATTGTTGTTGCCTCTCTTGCCGTCGGTACCATACATAGAAATGTTACTACAAGTGTTYGTTGTTGTTGTTGTAAACTTTGGGGATACCGAAACAGTTAAGATTGTTAAGGTGAGACAGAAAATCAATGGTGTCTAGATCCGGATCTTCCATTGCTCAATTTCAATGTGTTTCGCTTGACTGGTCTTTTGTTGCCATCTGACTCAAGTATTACTGAGCAACCCGA
TARGET=324,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05518 Ps_contig_mira-and-tgicl-ass_franssen_38371
SEQUENCE=TCGGGGCCTTGGAACAAAACTCATAATGGACTTAAAAYCGGGGGGATAAGATAGAAACGAGAGCACTATACTATTCCTTCTTTCTTTCTCTGCAATTTTAAAAAACCACCGCCACCGCGCCGTGACTGAACAATGGGTTCGGCGGCAACAATGGCGATGACGGCGCTCTGTTTCACCAAATCAAACAAACTCACTTCTTCCTTCCGCACTCTCCCTCGCCTCACCTGCTCTTCCCTCTCCTCTTCTCCTTTCAACTTCAACATCTCCTTCTCTCCCTCTAACCCTAACCCTAAACCCGACCCTAAACCCGATAACCCGAACCAATTTCCCGACGACAGTGGCGGTAACATGATCCCGTGGATTATTCGAGGCGAAGATGGAAACTTCAAACTCTCGTCTGAACCTCCTCCATCCTTTCTCAAAGCCATGACCGATGCCTCAACAGCTACAAAAAAATCAGAAAAAAAACCAACTGCGAAAGCCAAGAAGAAAAATAGAAAAGGAAACAAAGGTCCGTGAGGTGGCGCCGCCGCAGTATTCCAAAGCGGCACGGAGATTCTACAATGAAAAAATCAAGGAGTCATGTACGCGTCTCAGCAAGGTTCTCGCCGCCTCTGGAGTTGCGTCCAGGAGAAGCTGCGAAGAGCTTATTTTTAAAGGCAAGGTTACGGTTAACGGTTCCATCTGTAATATACCCCAGACTAAAGTGGATCCCTCAAAGGATATTATCTATGTCAATGGGAGCCGCCTTCCTAAGAGGCAGCCTCACAAAGTTTATCTTGCCTTGAACAAGCCAAAAGGTTACATATGCTCATCTGGGGAAAAGGAGTCTAAATCTGTTATAAGTCTATTTGATGATTTTTTGAGCAGTTGGGATAAGAAGCATCCAGGAGTACCCCCACCACGACTATTTACTGTAGGGCGCCTTGATGTTGCTACCACTGGGTTACTTATTGTGACTAATGATGGAGATTTTGCTCAAAAACTTTCACATCCATCGTCTAATTTTTCTAAAGAATACATTGCAACAGTTAATGGTCTAGTTCATAAGAGGCACTTAATGACCATAAGTGAAGGAACAACAATTGAGGGTGTCCATTGTGTACCAGATAGTGTGGGGTTACTTCCACGTATGCCAGATACGCAAAGATCTCGACTTCGTATTGTGGTTCATGATGGAAGGAAGCATGAAGTTCGCGAACTTGTTAAAAATGCTGGACTTGAGATTCACTCGTTAAAGCGAATTCGCATAGGTGGTTTTAGACTTCCACCAGACCTTGGGCTAGGGAAGTACATTGAGCTAAATCCTACGAATCTGAAAGCACTGGGAGGAAAAGTTAACAAAGTTAACTCGTAATTGTTGGGGTTGTAAATCTTCCTCCTCCATATTCAGATTTTGACAGCTCTGAAGCTGTTATGGTGGAATTGGCCCAATGCTTGTTTGATTTGAGCTGTTGAATGTTGAATTCTGATACCTGGTGCATTCCTTGGGAAGTATTTGGAATAGCCACAGATCACAAATTTGTGGAGTGTTTGGCATCAATGTAAACTGAGGCTCTTGAAATCAAACATGAAACATAGTTACATAGTTTAGAAGAATTCAGTAAAGATTTTAGACATTAATGTATGTATGTATGTATAATGCTTTGTAAAATGTAAATGGTGGTAATATTCCTGTGATAGTGAATGATTTTAGTTTAAGGT
TARGET=78,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05519 Ps_contig_mira-and-tgicl-ass_franssen_38371
SEQUENCE=TCGGGGCCTTGGAACAAAACTCATAATGGACTTAAAAYCGGGGGGATAAGATAGAAACGAGAGCACTATACTATTCCTTCTTTCTTTCTCTGCAATTTTAAAAAACCACCGCCACCGCGCCGTGACTGAACAATGGGTTCGGCGGCAACAATGGCGATGACGGCGCTCTGTTTCACCAAATCAAACAAACTCACTTCTTCCTTCCGCACTCTCCCTCGCCTCACCTGCTCTTCCCTCTCCTCTTCTCCTTTCAACTTCAACATCTCCTTCTCTCCCTCTAACCCTAACCCTAAACCCGACCCTAAACCCGATAACCCGAACCAATTTCCCGACGACAGTGGCGGTAACATGATCCCGTGGATTATTCGAGGCGAAGATGGAAACTTCAAACTCTCGTCTGAACCTCCTCCATCCTTTCTCAAAGCCATGACCGATGCCTCAACAGCTACAAAAAAATCAGAAAAAAAACCAACTGCGAAAGCCAAGAAGAAAAATAGAAAAGGAAACAAAGGTCCGTGAGGTGGCGCCGCCGCAGTATTCCAAAGCGGCACGGAGATTCTACAATGAAAAAATCAAGGAGTCATGTACGCGTCTCAGCAAGGTTCTCGCCGCCTCTGGAGTTGCGTCCAGGAGAAGCTGCGAAGAGCTTATTTTTAAAGGCAAGGTTACGGTTAACGGTTCCATCTGTAATATACCCCAGACTAAAGTGGATCCCTCAAAGGATATTATCTATGTCAATGGGAGCCGCCTTCCTAAGAGGCAGCCTCACAAAGTTTATCTTGCCTTGAACAAGCCAAAAGGTTACATATGCTCATCTGGGGAAAAGGAGTCTAAATCTGTTATAAGTCTATTTGATGATTTTTTGAGCAGTTGGGATAAGAAGCATCCAGGAGTACCCCCACCACGACTATTTACTGTAGGGCGCCTTGATGTTGCTACCACTGGGTTACTTATTGTGACTAATGATGGAGATTTTGCTCAAAAACTTTCACATCCATCGTCTAATTTTTCTAAAGAATACATTGCAACAGTTAATGGTCTAGTTCATAAGAGGCACTTAATGACCATAAGTGAAGGAACAACAATTGAGGGTGTCCATTGTGTACCAGATAGTGTGGGGTTACTTCCACGTATGCCAGATACGCAAAGATCTCGACTTCGTATTGTGGTTCATGATGGAAGGAAGCATGAAGTTCGCGAACTTGTTAAAAATGCTGGACTTGAGATTCACTCGTTAAAGCGAATTCGCATAGGTGGTTTTAGACTTCCACCAGACCTTGGGCTAGGGAAGTACATTGAGCTAAATCCTACGAATCTGAAAGCACTGGGAGGAAAAGTTAACAAAGTTAACTCGTAATTGTTGGGGTTGTAAATCTTCCTCCTCCATATTCAGATTTTGACAGCTCTGAAGCTGTTATGGTGGAATTGGCCCAATGCTTGTTTGATTTGAGCTGTTGAATGTTGAATTCTGATACCTGGTGCATTCCTTGGGAAGTATTTGGAATAGCCACAGATCACAAATTTGTGGAGTGTTTGGCATCAATGTAAACTGAGGCTCTTGAAATCAAACATGAAACATAGTTACATAGTTTAGAAGAATTCAGTAAAGATTTTAGACATTAATGTATGTATGTATGTATAATGCTTTGTAAAATGTAAATGGTGGTAATATTCCTGTGATAGTGAATGATTTTAGTTTAAGGT
TARGET=1623,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05520 Ps_contig_mira-and-tgicl-ass_franssen_38376
SEQUENCE=CTAACATCATCATCATCCTCCGATGTATGATTCACGGCGCCAGAGTTCGAAATCAAGAGCCAAGTACGAGGACGAACATGCTCGCTTGCTTCACGCCTCACTCACTCACGTCATGAAGCTGGGATGGACAGAAGCAGCGTTAATGGCGAGGGGGCGAAACGATTGGTTGGTTCTTTTCCTTCCTTCTTATTTGGTTGGTATTC
TARGET=5,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05521 Ps_contig_mira-and-tgicl-ass_franssen_38376
SEQUENCE=CTAACATCATCATCATCCTCCGATGTATGATTCACGGCGCCAGAGTTCGAAATCAAGAGCCAAGTACGAGGACGAACATGCTCGCTTGCTTCACGCCTCACTCACTCACGTCATGAAGCTGGGATGGACAGAAGCAGCGTTAATGGCGAGGGGGCGAAACGATTGGTTGGTTCTTTTCCTTCCTTCTTATTTGGTTGGTATTC
TARGET=97,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5425
PRIMER_LEFT_PENALTY=0.221115
PRIMER_RIGHT_PENALTY=0.321382
PRIMER_LEFT_SEQUENCE=ATCAAGAGCCAAGTACGAGGAC
PRIMER_RIGHT_SEQUENCE=GAAGGAAGGAAAAGAACCAACC
PRIMER_LEFT=51,22
PRIMER_RIGHT=185,22
PRIMER_LEFT_TM=59.779
PRIMER_RIGHT_TM=60.321
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=0.5834
PRIMER_LEFT_1_PENALTY=0.261998
PRIMER_RIGHT_1_PENALTY=0.321382
PRIMER_LEFT_1_SEQUENCE=AATCAAGAGCCAAGTACGAGGA
PRIMER_RIGHT_1_SEQUENCE=GAAGGAAGGAAAAGAACCAACC
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=60.262
PRIMER_RIGHT_1_TM=60.321
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.6173
PRIMER_LEFT_2_PENALTY=0.295944
PRIMER_RIGHT_2_PENALTY=0.321382
PRIMER_LEFT_2_SEQUENCE=TAACATCATCATCATCCTCCGA
PRIMER_RIGHT_2_SEQUENCE=GAAGGAAGGAAAAGAACCAACC
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=185,22
PRIMER_LEFT_2_TM=60.296
PRIMER_RIGHT_2_TM=60.321
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=185
PRIMER_PAIR_PENALTY_3=0.6939
PRIMER_LEFT_3_PENALTY=0.372557
PRIMER_RIGHT_3_PENALTY=0.321382
PRIMER_LEFT_3_SEQUENCE=AGAGTTCGAAATCAAGAGCCAA
PRIMER_RIGHT_3_SEQUENCE=GAAGGAAGGAAAAGAACCAACC
PRIMER_LEFT_3=41,22
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=60.373
PRIMER_RIGHT_3_TM=60.321
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=0.6939
PRIMER_LEFT_4_PENALTY=0.372557
PRIMER_RIGHT_4_PENALTY=0.321382
PRIMER_LEFT_4_SEQUENCE=GAGTTCGAAATCAAGAGCCAAG
PRIMER_RIGHT_4_SEQUENCE=GAAGGAAGGAAAAGAACCAACC
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=60.373
PRIMER_RIGHT_4_TM=60.321
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_05522 Ps_contig_mira-and-tgicl-ass_franssen_38377
SEQUENCE=CGGTATCAACGCAGAGTACTGTTCTACATGGTCGGGCGGAATGTGGAAGCTTGGAAATCTTTTGATAACGCTGTCATCAAGCTGAGAGCCGGCGGGGAGAGGAAATCTGCGTTTTTCGGATTCGTGTTGAACCAGATGGGCTTGGTGTGTGTTCAACTCTATAAAATAGAAGAAGCTGCTACACATTTTGAAGAAGCTAGAGAGATACTGGAGAGGGAATGTGGAAACTATCATTTGGACACTCTTGGAGTGTATAGCAACCTAGCTGCAACTTATGATGCATTAGGAAGAGTTGAAGATGCGATTCGAATATTGGAATACATACTGAAAATGCGAGAAGAAAAACTTGGAACTGCGAATCCAGATGTTGATGAAGAAAAGAAAAGACTGTTTGAGCTTTTGAAAGAAGCGGGAAGAGTTCGAAGTAGAAAGGGAAAATCTCTCGAAAACCTTATCGATTCAAACTCGTTGAAGATGAAGAAGGAAGGAAGAAGGAAATGGGGTGCATTTAGTTTTAGAACTTAAGAGATGTTTTTGTTCTTACAACAAAAAATTGGTCTCTTTTGTTTTGCATTTGTGAGAATTTGTGAGGCTTTTTGTTTGAAGATGCTTCCTTCTTCCTCATATATTTTAAGAGATGTCATTGTTCTTACAACAAAATTATTAAGTGTGTGTTTGAAAAACTTGGAACNGAAGTTACAAATACCAGTTTCTCAGTTTAAACT
TARGET=480,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2131
PRIMER_LEFT_PENALTY=0.048460
PRIMER_RIGHT_PENALTY=0.164639
PRIMER_LEFT_SEQUENCE=AAGGGAAAATCTCTCGAAAACC
PRIMER_RIGHT_SEQUENCE=GGAAGAAGGAAGCATCTTCAAA
PRIMER_LEFT=429,22
PRIMER_RIGHT=620,22
PRIMER_LEFT_TM=59.952
PRIMER_RIGHT_TM=59.835
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=0.2131
PRIMER_LEFT_1_PENALTY=0.048460
PRIMER_RIGHT_1_PENALTY=0.164639
PRIMER_LEFT_1_SEQUENCE=GGGAAAATCTCTCGAAAACCTT
PRIMER_RIGHT_1_SEQUENCE=GGAAGAAGGAAGCATCTTCAAA
PRIMER_LEFT_1=431,22
PRIMER_RIGHT_1=620,22
PRIMER_LEFT_1_TM=59.952
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.2372
PRIMER_LEFT_2_PENALTY=0.072552
PRIMER_RIGHT_2_PENALTY=0.164639
PRIMER_LEFT_2_SEQUENCE=AGAAGAAAAACTTGGAACTGCG
PRIMER_RIGHT_2_SEQUENCE=GGAAGAAGGAAGCATCTTCAAA
PRIMER_LEFT_2=335,22
PRIMER_RIGHT_2=620,22
PRIMER_LEFT_2_TM=59.927
PRIMER_RIGHT_2_TM=59.835
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=0.2784
PRIMER_LEFT_3_PENALTY=0.113803
PRIMER_RIGHT_3_PENALTY=0.164639
PRIMER_LEFT_3_SEQUENCE=CGGGAAGAGTTCGAAGTAGAAA
PRIMER_RIGHT_3_SEQUENCE=GGAAGAAGGAAGCATCTTCAAA
PRIMER_LEFT_3=409,22
PRIMER_RIGHT_3=620,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=59.835
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.2804
PRIMER_LEFT_4_PENALTY=0.115769
PRIMER_RIGHT_4_PENALTY=0.164639
PRIMER_LEFT_4_SEQUENCE=GAAAAACTTGGAACTGCGAATC
PRIMER_RIGHT_4_SEQUENCE=GGAAGAAGGAAGCATCTTCAAA
PRIMER_LEFT_4=339,22
PRIMER_RIGHT_4=620,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=59.835
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_05523 Ps_contig_mira-and-tgicl-ass_franssen_38388
SEQUENCE=CTTCAATTGTTTAAAATTATGATCATCATTCAAACCCAAGTCACCATAATGAGCGTCATAATAGAAACCCTCCCCTCGGGTAGTACAAGGCCCAATGCAAAGCTTGCATCCATATTCCGTTTCAAGAGACTGGCCAAGAATGTGAGCACTAGAATGCCAAAAAGTGTCACGTCCTTCATCATCATCAAACTTAAATATCTGAAGTAACGAATCCTCTTCAAGAGGACGATTCATATCCCACAGGACACCATTGACCTTAGAAATAAGAGCATTATTCGCTAAATTCTTAGAGATTTCACGAGCCACATC
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4499
PRIMER_LEFT_PENALTY=0.163658
PRIMER_RIGHT_PENALTY=0.286239
PRIMER_LEFT_SEQUENCE=TCATCATTCAAACCCAAGTCAC
PRIMER_RIGHT_SEQUENCE=GGGATATGAATCGTCCTCTTGA
PRIMER_LEFT=22,22
PRIMER_RIGHT=238,22
PRIMER_LEFT_TM=59.836
PRIMER_RIGHT_TM=60.286
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=217
PRIMER_PAIR_PENALTY_1=0.5116
PRIMER_LEFT_1_PENALTY=0.225403
PRIMER_RIGHT_1_PENALTY=0.286239
PRIMER_LEFT_1_SEQUENCE=AATGTGAGCACTAGAATGCCAA
PRIMER_RIGHT_1_SEQUENCE=GGGATATGAATCGTCCTCTTGA
PRIMER_LEFT_1=138,22
PRIMER_RIGHT_1=238,22
PRIMER_LEFT_1_TM=59.775
PRIMER_RIGHT_1_TM=60.286
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.5278
PRIMER_LEFT_2_PENALTY=0.241544
PRIMER_RIGHT_2_PENALTY=0.286239
PRIMER_LEFT_2_SEQUENCE=TTTCAAGAGACTGGCCAAGAAT
PRIMER_RIGHT_2_SEQUENCE=GGGATATGAATCGTCCTCTTGA
PRIMER_LEFT_2=119,22
PRIMER_RIGHT_2=238,22
PRIMER_LEFT_2_TM=60.242
PRIMER_RIGHT_2_TM=60.286
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=120
PRIMER_PAIR_PENALTY_3=0.5353
PRIMER_LEFT_3_PENALTY=0.249074
PRIMER_RIGHT_3_PENALTY=0.286239
PRIMER_LEFT_3_SEQUENCE=AACCCAAGTCACCATAATGAGC
PRIMER_RIGHT_3_SEQUENCE=GGGATATGAATCGTCCTCTTGA
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=238,22
PRIMER_LEFT_3_TM=60.249
PRIMER_RIGHT_3_TM=60.286
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=0.5620
PRIMER_LEFT_4_PENALTY=0.275749
PRIMER_RIGHT_4_PENALTY=0.286239
PRIMER_LEFT_4_SEQUENCE=CATTCAAACCCAAGTCACCATA
PRIMER_RIGHT_4_SEQUENCE=GGGATATGAATCGTCCTCTTGA
PRIMER_LEFT_4=26,22
PRIMER_RIGHT_4=238,22
PRIMER_LEFT_4_TM=59.724
PRIMER_RIGHT_4_TM=60.286
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_05524 Ps_contig_mira-and-tgicl-ass_franssen_38396
SEQUENCE=ATNTTTTTCTAGATTATTAACCATTACAGTTATATCAACTAGTTGTGATAGATAGTTTCACACACAACACAACACAAACCAAACATCATAACATACCTAACATACCATGACATGTAACACAACATCATCATCGTCAACAACAACACCAACAACCTCAAACTAATCAAACCATCCAAACATGATCATGGACAGAACCGACTCCCCCACTCGCGACCAAAAAGTCGTATCGATCGAATGTCTGAGAGGAAGTTCCAAAGCCGATGAATGGACCGGAGACATGCTCCAAACCGGCGACATAGTCGAAGAGCTTCGAATCGGAACAAAATCGAACTCCCTCATCCGGTTCAACTCACCCTTCAAAGGTGGAAAATCTGGAATCCAGAAGATTCTTCAAGAAGCTTAAACAA
TARGET=62,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05525 Ps_contig_mira-and-tgicl-ass_franssen_38399
SEQUENCE=CCGGTTTACAAGTACAACTCCCCACCTCCTCCAACACCGGTTTACAAGTACAAGTCACCACCTCCACCGTCACCAACTTACGAGTACAAATCTCCTCCTCCTCCAACACCAGTTTACAAGTACAAATCTCCACCTCCACCATCACCTACATATGTGTACAAGTCTCCTCCACCACCAACACCAGTTTACAAGTACAACTCACCACCTCCACCATCCCCATCACATCCCCCTCCATACTATTACAATTCTCCCCCACCGCCATCACCATCCCCACCTCCACCTTACTATTACCAATCCCCACCACCACCATCCTCTTCTCCACCACCTCCTTATTACTATCAAAGTCCTCCTCCCCCGTCTCCCACTCCTCATACTCCCTACTACTACAAATCCCCTCCACCACCAACGTCATCTCCTCCACCTCCTTACCACTATGTGAGTCCTCCCCCACCAACATCATCTCCTCCCCCACCATACCACTACACATCGCCACCTCCTCCCTCCCCGTCTCCTGCTCCCACATACATTTACAAATCACCTCCCCCACCAGTGAAATCGCCTCCCCCACCAGCTTACATTTATGCATCCCCACCACCACCTATCTACAAGTAAGCTCAAAATGCAGCAACACCGTTCCGTATAAATCATGTTTTAGTTTTCATCAAGGAATAAATGCTACCTCCATTTGGAACAAAGTGAAACTACCTTCCAGTCTAAGTTCTTTGAATAAAAGTCTGAATCTATTATCTCTACGCAACATTATTTGTTTTTCAGACAAAATTGAGTCTATTTCTTTGGTGGTGATCTTGCACGTCCTGGTCTTGTTGTGCTCAAGTGACATACAAATCAAAAGGAAGCAATATTTGAATGTAAATGTCAAGTGAAAGTCATAAGTACAGTCCACTTTTAGTGATCTCTTTAATTTATTTGTGCATAGATGAATATGGTCGTGTATGATTGCTATTATATTTATTGTGGCAATTTTGTATTTGTGTTTGAAATAAGAGTGCTAGTAACCAAGGAGAACATTAGATTTCTATTACAAAAAATTTGTGTTTAATTTACC
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1210
PRIMER_LEFT_PENALTY=0.088479
PRIMER_RIGHT_PENALTY=0.032476
PRIMER_LEFT_SEQUENCE=CCGTCACCAACTTACGAGTACA
PRIMER_RIGHT_SEQUENCE=ATAGTAAGGTGGAGGTGGGGAT
PRIMER_LEFT=66,22
PRIMER_RIGHT=287,22
PRIMER_LEFT_TM=60.088
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.2338
PRIMER_LEFT_1_PENALTY=0.201297
PRIMER_RIGHT_1_PENALTY=0.032476
PRIMER_LEFT_1_SEQUENCE=CCGGTTTACAAGTACAACTCCC
PRIMER_RIGHT_1_SEQUENCE=ATAGTAAGGTGGAGGTGGGGAT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=287,22
PRIMER_LEFT_1_TM=59.799
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.5342
PRIMER_LEFT_2_PENALTY=0.088479
PRIMER_RIGHT_2_PENALTY=0.445740
PRIMER_LEFT_2_SEQUENCE=CCGTCACCAACTTACGAGTACA
PRIMER_RIGHT_2_SEQUENCE=AATAGTATGGAGGGGGATGTGA
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=240,22
PRIMER_LEFT_2_TM=60.088
PRIMER_RIGHT_2_TM=59.554
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=175
PRIMER_PAIR_PENALTY_3=0.9325
PRIMER_LEFT_3_PENALTY=0.201297
PRIMER_RIGHT_3_PENALTY=0.731168
PRIMER_LEFT_3_SEQUENCE=CGGTTTACAAGTACAACTCCCC
PRIMER_RIGHT_3_SEQUENCE=TTGTAAACTGGTGTTGGTGGTG
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=190,22
PRIMER_LEFT_3_TM=59.799
PRIMER_RIGHT_3_TM=60.731
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=190
PRIMER_PAIR_PENALTY_4=0.9412
PRIMER_LEFT_4_PENALTY=0.201297
PRIMER_RIGHT_4_PENALTY=0.739871
PRIMER_LEFT_4_SEQUENCE=CCGGTTTACAAGTACAACTCCC
PRIMER_RIGHT_4_SEQUENCE=TAATAGTAAGGTGGAGGTGGGG
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=289,22
PRIMER_LEFT_4_TM=59.799
PRIMER_RIGHT_4_TM=59.260
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_05526 Ps_contig_mira-and-tgicl-ass_franssen_38399
SEQUENCE=CCGGTTTACAAGTACAACTCCCCACCTCCTCCAACACCGGTTTACAAGTACAAGTCACCACCTCCACCGTCACCAACTTACGAGTACAAATCTCCTCCTCCTCCAACACCAGTTTACAAGTACAAATCTCCACCTCCACCATCACCTACATATGTGTACAAGTCTCCTCCACCACCAACACCAGTTTACAAGTACAACTCACCACCTCCACCATCCCCATCACATCCCCCTCCATACTATTACAATTCTCCCCCACCGCCATCACCATCCCCACCTCCACCTTACTATTACCAATCCCCACCACCACCATCCTCTTCTCCACCACCTCCTTATTACTATCAAAGTCCTCCTCCCCCGTCTCCCACTCCTCATACTCCCTACTACTACAAATCCCCTCCACCACCAACGTCATCTCCTCCACCTCCTTACCACTATGTGAGTCCTCCCCCACCAACATCATCTCCTCCCCCACCATACCACTACACATCGCCACCTCCTCCCTCCCCGTCTCCTGCTCCCACATACATTTACAAATCACCTCCCCCACCAGTGAAATCGCCTCCCCCACCAGCTTACATTTATGCATCCCCACCACCACCTATCTACAAGTAAGCTCAAAATGCAGCAACACCGTTCCGTATAAATCATGTTTTAGTTTTCATCAAGGAATAAATGCTACCTCCATTTGGAACAAAGTGAAACTACCTTCCAGTCTAAGTTCTTTGAATAAAAGTCTGAATCTATTATCTCTACGCAACATTATTTGTTTTTCAGACAAAATTGAGTCTATTTCTTTGGTGGTGATCTTGCACGTCCTGGTCTTGTTGTGCTCAAGTGACATACAAATCAAAAGGAAGCAATATTTGAATGTAAATGTCAAGTGAAAGTCATAAGTACAGTCCACTTTTAGTGATCTCTTTAATTTATTTGTGCATAGATGAATATGGTCGTGTATGATTGCTATTATATTTATTGTGGCAATTTTGTATTTGTGTTTGAAATAAGAGTGCTAGTAACCAAGGAGAACATTAGATTTCTATTACAAAAAATTTGTGTTTAATTTACC
TARGET=298,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2792
PRIMER_LEFT_PENALTY=0.032476
PRIMER_RIGHT_PENALTY=0.246726
PRIMER_LEFT_SEQUENCE=ATCCCCACCTCCACCTTACTAT
PRIMER_RIGHT_SEQUENCE=GGGGAGGACTCACATAGTGGTA
PRIMER_LEFT=266,22
PRIMER_RIGHT=447,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.247
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.3000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=182
PRIMER_PAIR_PENALTY_1=0.3127
PRIMER_LEFT_1_PENALTY=0.065960
PRIMER_RIGHT_1_PENALTY=0.246726
PRIMER_LEFT_1_SEQUENCE=CAAGTACAACTCACCACCTCCA
PRIMER_RIGHT_1_SEQUENCE=GGGGAGGACTCACATAGTGGTA
PRIMER_LEFT_1=188,22
PRIMER_RIGHT_1=447,22
PRIMER_LEFT_1_TM=60.066
PRIMER_RIGHT_1_TM=60.247
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=260
PRIMER_PAIR_PENALTY_2=0.5776
PRIMER_LEFT_2_PENALTY=0.330865
PRIMER_RIGHT_2_PENALTY=0.246726
PRIMER_LEFT_2_SEQUENCE=AGTACAACTCACCACCTCCACC
PRIMER_RIGHT_2_SEQUENCE=GGGGAGGACTCACATAGTGGTA
PRIMER_LEFT_2=190,22
PRIMER_RIGHT_2=447,22
PRIMER_LEFT_2_TM=60.331
PRIMER_RIGHT_2_TM=60.247
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.6925
PRIMER_LEFT_3_PENALTY=0.445740
PRIMER_RIGHT_3_PENALTY=0.246726
PRIMER_LEFT_3_SEQUENCE=TCACATCCCCCTCCATACTATT
PRIMER_RIGHT_3_SEQUENCE=GGGGAGGACTCACATAGTGGTA
PRIMER_LEFT_3=219,22
PRIMER_RIGHT_3=447,22
PRIMER_LEFT_3_TM=59.554
PRIMER_RIGHT_3_TM=60.247
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=229
PRIMER_PAIR_PENALTY_4=0.7222
PRIMER_LEFT_4_PENALTY=0.032476
PRIMER_RIGHT_4_PENALTY=0.689754
PRIMER_LEFT_4_SEQUENCE=ATCCCCACCTCCACCTTACTAT
PRIMER_RIGHT_4_SEQUENCE=AGGTGGCGATGTGTAGTGGTAT
PRIMER_LEFT_4=266,22
PRIMER_RIGHT_4=494,22
PRIMER_LEFT_4_TM=59.968
PRIMER_RIGHT_4_TM=60.690
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=6.8000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_05527 Ps_contig_mira-and-tgicl-ass_franssen_38401
SEQUENCE=CCTTTTCTCATTTGCAAGAACACAACCTGGGCGGCTTTGTGGGCTTAACTGCAGTTTTCATTTTCCTTTGGCTTTGCAGCTTGCGTTTCCTGACTTGCGCGTCCTGTTATAGCAGTTGCTTTCACTTTTGGCTTCGTCTGTAGCTGGGTTGTCGCAGCTTATGAAGCGTGCGTTTGGTTGGTTGGCGCTACGGACCCGCGTTCTACTGCATTTTGAATTTATTTTGGTTTATTTTGCTATTTTTTGATGCAGCTGGTGTGGTGTAATATGGCTTGCAAGATGAAGTTCGGTTGTTGCGTTCTACTTATGCAGCTTACTATTTACGG
TARGET=174,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2922
PRIMER_LEFT_PENALTY=0.066394
PRIMER_RIGHT_PENALTY=0.225759
PRIMER_LEFT_SEQUENCE=AGTTGCTTTCACTTTTGGCTTC
PRIMER_RIGHT_SEQUENCE=AGTAGAACGCAACAACCGAACT
PRIMER_LEFT=113,22
PRIMER_RIGHT=304,22
PRIMER_LEFT_TM=59.934
PRIMER_RIGHT_TM=60.226
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=0.3061
PRIMER_LEFT_1_PENALTY=0.080323
PRIMER_RIGHT_1_PENALTY=0.225759
PRIMER_LEFT_1_SEQUENCE=TTCACTTTTGGCTTCGTCTGTA
PRIMER_RIGHT_1_SEQUENCE=AGTAGAACGCAACAACCGAACT
PRIMER_LEFT_1=120,22
PRIMER_RIGHT_1=304,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=60.226
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.3740
PRIMER_LEFT_2_PENALTY=0.080323
PRIMER_RIGHT_2_PENALTY=0.293633
PRIMER_LEFT_2_SEQUENCE=TTCACTTTTGGCTTCGTCTGTA
PRIMER_RIGHT_2_SEQUENCE=CATAAGTAGAACGCAACAACCG
PRIMER_LEFT_2=120,22
PRIMER_RIGHT_2=308,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=59.706
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.3798
PRIMER_LEFT_3_PENALTY=0.154023
PRIMER_RIGHT_3_PENALTY=0.225759
PRIMER_LEFT_3_SEQUENCE=TCTCATTTGCAAGAACACAACC
PRIMER_RIGHT_3_SEQUENCE=AGTAGAACGCAACAACCGAACT
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=304,22
PRIMER_LEFT_3_TM=60.154
PRIMER_RIGHT_3_TM=60.226
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.5154
PRIMER_LEFT_4_PENALTY=0.066394
PRIMER_RIGHT_4_PENALTY=0.449009
PRIMER_LEFT_4_SEQUENCE=AGTTGCTTTCACTTTTGGCTTC
PRIMER_RIGHT_4_SEQUENCE=TATTACACCACACCAGCTGCAT
PRIMER_LEFT_4=113,22
PRIMER_RIGHT_4=267,22
PRIMER_LEFT_4_TM=59.934
PRIMER_RIGHT_4_TM=60.449
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_05528 Ps_contig_mira-and-tgicl-ass_franssen_38408
SEQUENCE=TTAAAGTACATAAACATAAACCTAAANARARAGACAAGCTAATTGTATGTTTGAATTCGCAGCGGGAATCGTAGAATCGCAGCGAGTCACTGCGATTTTATCAAACTCGCTGCTCGTCCGAACATACTCACACTAAGTGTTTCCTAACCTCAACACACCAAAATCTAAAATAAAAAGAACTTCCTAAAGCATTTATGCCTCTGATTTCCTATGAAAACCACTTCAAAAGTCCATCTTCAAAAGTGTGATTATTTTATATGTATCTTGCTATTAACATACACACAACAATAATCAAACAGAAACATCAGGAAGTGTCACCAAGAAAAAGTTATCCAAATCAGGAGGTACAAAGAATGAAAGTGGTGACCTTCTCAAAGAACAATTTGAAACTACCCTTTGCTTCTTTGGTGCTGGAGGACATGTTGAAACCTCTGGTATCCTAAATTTCTGACCTTTTGGTGTTGAACATGCACTGTTACTAACAGAAACATCAATTACCTCAGAAACTTCGTTGTTCTCTTCCCTTTCTTCCTTTGAAGATTCTTCAGAGCTTTTTCTCACACAGCTAGAAGGCAAAACCTTGTTAATCACCTYCTTYGTTTTTTGTTCCTCTTGGAGCATTGTTCTTCTTAGGTTGTTCTTTGTTTCTTTGTTTCTTCTTCTGTTGTTGTTTGTGTCTGNTTGTTTTGTTTGTTGTTGTTGTTAGTTAAGTCGAGGTATTACAAGTAAAAG
TARGET=691,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05529 Ps_contig_mira-and-tgicl-ass_franssen_38409
SEQUENCE=TGGAGCCTTTTCTCCATGCTCTGAAACAATATTTTCTACTGCAAACCCAGAGGTGGCCTTCCTAAGTTGATCAACCGTATATTCACGAAACGACGGCGGGCCATTGCCCTCAATATTATTGTTTTCATTTTCATCAATGTCAGTCTCCGGAACCTGAACACCACCACCACCACCACCACCACCACCACCTTGTTCACTACCCCAACAGCATGTTGAATACTTTGAGAACTTACAACCCATGCCAACCTTAGAGAAGAAAGGGATTCTAAACAGTCAAAA
TARGET=159,30
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2242
PRIMER_LEFT_PENALTY=0.110392
PRIMER_RIGHT_PENALTY=0.113803
PRIMER_LEFT_SEQUENCE=TTTTCATCAATGTCAGTCTCCG
PRIMER_RIGHT_SEQUENCE=CCTTTCTTCTCTAAGGTTGGCA
PRIMER_LEFT=127,22
PRIMER_RIGHT=260,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=134
PRIMER_PAIR_PENALTY_1=0.2242
PRIMER_LEFT_1_PENALTY=0.110393
PRIMER_RIGHT_1_PENALTY=0.113803
PRIMER_LEFT_1_SEQUENCE=CAACCGTATATTCACGAAACGA
PRIMER_RIGHT_1_SEQUENCE=CCTTTCTTCTCTAAGGTTGGCA
PRIMER_LEFT_1=71,22
PRIMER_RIGHT_1=260,22
PRIMER_LEFT_1_TM=59.890
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=190
PRIMER_PAIR_PENALTY_2=0.3553
PRIMER_LEFT_2_PENALTY=0.241544
PRIMER_RIGHT_2_PENALTY=0.113803
PRIMER_LEFT_2_SEQUENCE=CCTTTTCTCCATGCTCTGAAAC
PRIMER_RIGHT_2_SEQUENCE=CCTTTCTTCTCTAAGGTTGGCA
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=260,22
PRIMER_LEFT_2_TM=60.242
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.3927
PRIMER_LEFT_3_PENALTY=0.110392
PRIMER_RIGHT_3_PENALTY=0.282356
PRIMER_LEFT_3_SEQUENCE=TTTTCATCAATGTCAGTCTCCG
PRIMER_RIGHT_3_SEQUENCE=GTTGGCATGGGTTGTAAGTTCT
PRIMER_LEFT_3=127,22
PRIMER_RIGHT_3=245,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=60.282
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=0.3927
PRIMER_LEFT_4_PENALTY=0.110393
PRIMER_RIGHT_4_PENALTY=0.282356
PRIMER_LEFT_4_SEQUENCE=CAACCGTATATTCACGAAACGA
PRIMER_RIGHT_4_SEQUENCE=GTTGGCATGGGTTGTAAGTTCT
PRIMER_LEFT_4=71,22
PRIMER_RIGHT_4=245,22
PRIMER_LEFT_4_TM=59.890
PRIMER_RIGHT_4_TM=60.282
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_05530 Ps_contig_mira-and-tgicl-ass_franssen_38428
SEQUENCE=TCAAGTTCATCATCGCTGCCACTTGCCCTCGTGACTCATCAATTGCNTAAGCNCCCAAAATACTTCTATGTCANTTCCTCTTCAAGTTCATCNTAAGCACCTTCTACATACTCTATTTCTTCTTCCATTTCATCCTCTTCTTCAGCAGGTTGACTTTTCTCCATTTCAATAATTTTATTGTATTCTTCAAAAGGATAGTTGATGATATCATCTTGCTTGTAAAGTCCTTCTTTAAGGCGTGCCAATAGCTCTTTCTCAATAGACTTTTCTAGCACAGCTGCTTTTTCGGCCTTCTCTTCTCTTCTAGCTTCTCTCTTTACCTCCTTCCTAGGCAATGTCGTTATTTTCTCCCTTGTTTTCAAAGCAAGCTTCCTCATACGTATACGCATCTGGGTCATTTTGGTCAACCGTTGTTTTATCTTGTGTAGAAGAATCTTGGGCCAATACATCAGTATTTTTGTCTATGACTTCAAGTGCCTGCTCGTAATTCCTAGGCAGCTTAACTCTTTCCCACAAATCCTTTGGCATATGAGCTCTTTCAATAGTTTTCATGTAAAGGTAAAACACACCATTATCTTCGCGAATGGTGGCATATCGACTATTAGCTAAAGGACAGGAACTTCGGTTGCAAATTCCAGTGATGCTGTAAGGGTTTCTACAAAAAATACCTGTCGTAATTTTTGCCATGAAACTGCAATGGTTGTGCCTGATAACTTGCCATATAACCTCATCGTGCTGCATCTTTGGAATGAAATTTTTAGGGTTTTAACTTTGGGTGTTGAGGTTTTAGGGTGAAAACGAATGGATTGGATGGAAGGAAAACCGAAAGGGTGCTGTGTGAGGGAGGTGCGACGGCCGACTAAATGATGATGGAAACTGGGCTAGGTTTCAGAACCTTGCTGCTACTNCYCNACACTGTTGCCCCGAC
TARGET=291,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05531 Ps_contig_mira-and-tgicl-ass_franssen_38429
SEQUENCE=CTATATTTTCTAAAACTAAGAACCAACATAAAGATGAGTTCATGGTTCATTAAACCTTAACAAAAGGTCATGGCTGACTGGGATATGTGTTTATGTAATGTTATAGGCTGGTTATCTTGGCTAGTAGTTTAATCCTACAACATTTTTCTCTTTTTAGCATGCGATACTGCAGACCTTATTGCAGGATTATCAAGAAACGATGAATCATCGGAGGAAGAAGAACTAGAGCTTTCACTGTCGCTGAAACAAATTTTTCTTTTTACCATGTCAAGTGGATTTGCAAATTGCCATGCTACTGAAGAACTTGGAGTTGGATCCTCAAACTTCATCGCAATTCCAGAAATAGCCTTCTTTTCATGTACTCCTCGAGAGATACACTGATCATCATCATCATCATCATCATCAAGGTTTATGATCTCAATCTTCTTTTCCAAATTCTTTGTTCCCTTACCTGGTGATGCACCTTGAGCATCCTTGTCCCCTTTGTTCTGTGGCTGAGTACTGCCAGCAACTCCAAGAGAATAGTGAGCATCTTCAGTCCTTCTCCACAGTGTCATTTACGAAACTCGTNCCAACAAACNTCATCATTCTTTGTCTTACAAAATCAAGTAACAACTGGTT
TARGET=381,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1387
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.115189
PRIMER_LEFT_SEQUENCE=AACTTGGAGTTGGATCCTCAAA
PRIMER_RIGHT_SEQUENCE=GTGGAGAAGGACTGAAGATGCT
PRIMER_LEFT=301,22
PRIMER_RIGHT=548,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.885
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.2480
PRIMER_LEFT_1_PENALTY=0.132857
PRIMER_RIGHT_1_PENALTY=0.115189
PRIMER_LEFT_1_SEQUENCE=TTACCATGTCAAGTGGATTTGC
PRIMER_RIGHT_1_SEQUENCE=GTGGAGAAGGACTGAAGATGCT
PRIMER_LEFT_1=259,22
PRIMER_RIGHT_1=548,22
PRIMER_LEFT_1_TM=59.867
PRIMER_RIGHT_1_TM=59.885
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=0.2505
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.226971
PRIMER_LEFT_2_SEQUENCE=AACTTGGAGTTGGATCCTCAAA
PRIMER_RIGHT_2_SEQUENCE=GGNACGAGTTTCGTAAATGACA
PRIMER_LEFT_2=301,22
PRIMER_RIGHT_2=572,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=59.773
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.2505
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.226971
PRIMER_LEFT_3_SEQUENCE=AACTTGGAGTTGGATCCTCAAA
PRIMER_RIGHT_3_SEQUENCE=TGGNACGAGTTTCGTAAATGAC
PRIMER_LEFT_3=301,22
PRIMER_RIGHT_3=573,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=59.773
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.3371
PRIMER_LEFT_4_PENALTY=0.110131
PRIMER_RIGHT_4_PENALTY=0.226971
PRIMER_LEFT_4_SEQUENCE=AGAACTTGGAGTTGGATCCTCA
PRIMER_RIGHT_4_SEQUENCE=GGNACGAGTTTCGTAAATGACA
PRIMER_LEFT_4=299,22
PRIMER_RIGHT_4=572,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.773
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_05532 Ps_contig_mira-and-tgicl-ass_franssen_38434
SEQUENCE=CTGAAGGGTCTTTTTTGCAGGATCTTGATTCAAACAAGAAGCAACCATATCCTTAAAAGCCTTAGAAAATTTATTACTTCCACCACTATGACCCTTGTAAATATATTTATCAAAATCAGAAAACCTAAACCTCTTGGTGATGTTAAGCATCATTGACTTAGAAGGAGGAAGATGAGAAAGTGGTGGTCTTCCATGTGCCAACTCCAAAGCAGTTATCCCAAAAGACCAAATATCAGCTTTGAAACTGTAACCATTATGAGAATGAATAACCTCAGGAGCCATCCAATAAGGTGTTCCAGNAAAATCAGTAAACATAAGAGAAGAAGAAGAATTAGAAGATGAAGATGAATAAGAAGAACATGCTCCTACAGAGTTGTTTGATTCATAAATGGAGCAGAACACCAAATCAGCAAGTTTACACTAATCCATTTGAGTCAAACAAGGATGTTACCAGATTTAG
TARGET=319,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3611
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.360320
PRIMER_LEFT_SEQUENCE=AAGCAGTTATCCCAAAAGACCA
PRIMER_RIGHT_SEQUENCE=GATTTGGTGTTCTGCTCCATTT
PRIMER_LEFT=206,22
PRIMER_RIGHT=407,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.360
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=202
PRIMER_PAIR_PENALTY_1=0.3611
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.360320
PRIMER_LEFT_1_SEQUENCE=AGCAGTTATCCCAAAAGACCAA
PRIMER_RIGHT_1_SEQUENCE=GATTTGGTGTTCTGCTCCATTT
PRIMER_LEFT_1=207,22
PRIMER_RIGHT_1=407,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.360
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=201
PRIMER_PAIR_PENALTY_2=0.3611
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.360320
PRIMER_LEFT_2_SEQUENCE=ACTCCAAAGCAGTTATCCCAAA
PRIMER_RIGHT_2_SEQUENCE=GATTTGGTGTTCTGCTCCATTT
PRIMER_LEFT_2=200,22
PRIMER_RIGHT_2=407,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.360
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.3611
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=0.360320
PRIMER_LEFT_3_SEQUENCE=AACTCCAAAGCAGTTATCCCAA
PRIMER_RIGHT_3_SEQUENCE=GATTTGGTGTTCTGCTCCATTT
PRIMER_LEFT_3=199,22
PRIMER_RIGHT_3=407,22
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.360
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.3811
PRIMER_LEFT_4_PENALTY=0.020747
PRIMER_RIGHT_4_PENALTY=0.360320
PRIMER_LEFT_4_SEQUENCE=GATGAGAAAGTGGTGGTCTTCC
PRIMER_RIGHT_4_SEQUENCE=GATTTGGTGTTCTGCTCCATTT
PRIMER_LEFT_4=170,22
PRIMER_RIGHT_4=407,22
PRIMER_LEFT_4_TM=59.979
PRIMER_RIGHT_4_TM=60.360
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_05533 Ps_contig_mira-and-tgicl-ass_franssen_38435
SEQUENCE=GGGCTGTTAAAGAACGCACACTTGCTATTCTGCAAAAGCATCCACGTGTCACTAATTATGAACCAGAAAGTCCAATGAATTATGATTGTACCATTGCACATGTCAAGTGAAGTGGACAATCATTCCTTTATTGCAATGAACTAATGGCAGCTTGAACCCCTTTCGGTTTGGGTGTTTTCATGCTTTCTTCTTCCTTACCTGATCTTTCAGGTGACAAATTTTGGATGTATATACATACATACATACACGGAAACACATTTGGTGGTATATTTAAATGAGAGTTTGTACAATTTAGCAGTTTTAATATATTGAATTTGACTAACT
TARGET=231,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3910
PRIMER_LEFT_PENALTY=0.016221
PRIMER_RIGHT_PENALTY=0.374742
PRIMER_LEFT_SEQUENCE=CTGTTAAAGAACGCACACTTGC
PRIMER_RIGHT_SEQUENCE=TATACCACCAAATGTGTTTCCG
PRIMER_LEFT=3,22
PRIMER_RIGHT=268,22
PRIMER_LEFT_TM=59.984
PRIMER_RIGHT_TM=59.625
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=10.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.6390
PRIMER_LEFT_1_PENALTY=0.264300
PRIMER_RIGHT_1_PENALTY=0.374742
PRIMER_LEFT_1_SEQUENCE=CAATGAACTAATGGCAGCTTGA
PRIMER_RIGHT_1_SEQUENCE=TATACCACCAAATGTGTTTCCG
PRIMER_LEFT_1=133,22
PRIMER_RIGHT_1=268,22
PRIMER_LEFT_1_TM=60.264
PRIMER_RIGHT_1_TM=59.625
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.6981
PRIMER_LEFT_2_PENALTY=0.323355
PRIMER_RIGHT_2_PENALTY=0.374742
PRIMER_LEFT_2_SEQUENCE=AAAAGCATCCACGTGTCACTAA
PRIMER_RIGHT_2_SEQUENCE=TATACCACCAAATGTGTTTCCG
PRIMER_LEFT_2=33,22
PRIMER_RIGHT_2=268,22
PRIMER_LEFT_2_TM=59.677
PRIMER_RIGHT_2_TM=59.625
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=236
PRIMER_PAIR_PENALTY_3=0.7382
PRIMER_LEFT_3_PENALTY=0.363436
PRIMER_RIGHT_3_PENALTY=0.374742
PRIMER_LEFT_3_SEQUENCE=GGGTGTTTTCATGCTTTCTTCT
PRIMER_RIGHT_3_SEQUENCE=TATACCACCAAATGTGTTTCCG
PRIMER_LEFT_3=169,22
PRIMER_RIGHT_3=268,22
PRIMER_LEFT_3_TM=59.637
PRIMER_RIGHT_3_TM=59.625
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=1.0740
PRIMER_LEFT_4_PENALTY=0.699272
PRIMER_RIGHT_4_PENALTY=0.374742
PRIMER_LEFT_4_SEQUENCE=GGCTGTTAAAGAACGCACACTT
PRIMER_RIGHT_4_SEQUENCE=TATACCACCAAATGTGTTTCCG
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=268,22
PRIMER_LEFT_4_TM=60.699
PRIMER_RIGHT_4_TM=59.625
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_05534 Ps_contig_mira-and-tgicl-ass_franssen_38439
SEQUENCE=GATAAACAATACTAAAACAAACATCTATATATGAAACAAAACTGATTCACACACATTCATATTCAAATTCTCTCCACACAACAAAAATGAGCAAGGGACATAGTAAGAACATGTTTATGAGAATCATGTGTTCACCAATAAGAGCACTTGGAAAAGCAAGAGACGCATACGTAAGAAGCATAACAAACTGTGGTCAGAGCATGAATTACGGTAGTTATCCAATGGATGCCGCTAGTAGATTCTCTTCTTTATCAAGAAGTCAAACAACATCATCAAGAAGATCATCATCATCGTCATCATTATCAAGACTTGATAATAATGAAGATTTTGCTGAGCTTGTTAGAGCTGCTTCTGCAAGAACTTTGGGGAATAGGATGGACATAGATTTGGTTCTCAAACAAGAACAACAAAAGATGAATAAAAAGTTACCTAAGTCTAGTAGTGTTGGAATGGCTAAGATTGATGAAGATATGCCGTTTGATTCTGGTCTTGGGAATGGTGTTGGTTTTGTTGTTGATTCTTATCCTAGATCTAAGAGTTATGCTGTTGGAAATAGAACTAGGAATGGTGTTTTCTGAATCTATGTTGTTGAATTGAATGGAAGCTTTTTGGTGTTTGTGATATTTGTGAGGTGTTGTAAGAAATTATAAGAGAGGGATTTAATTGAATCTTAATCTCTYTTTAATTGATATATTGTGATTTGAATGGATAGTTGTAAATAATTTTTGTTTATTGTTTCT
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05535 Ps_contig_mira-and-tgicl-ass_franssen_38450
SEQUENCE=TCGGGGAAGGCAAAGAAAGCCCAATCGAACGAAAACAGAGTGTTTAGAAAAAGTAACTAACCAAACGGTCACTCTGATATGGCAATGACAGAACAACAAGACGAACCAGAAGAAGAACAACAACAACGACCATTGTTGCTGAATACTACAAAAGTTGTTGAGTATTTAGAGCCATTCATGTCATTAGAGCTTCTCTGCAAATTCCCRGACAACTCTGCTTATGATTTTGACTACTCTCAAAGCACAATCTGGTCCCCTTTAGTTCCTCGACCCTACTGCCCCATGGATTTGGATCTCATCACTCCGAAGAAACTTTCCTATGACTTTGGCTTAGGAGCTAGATGCAGTGTCAACAAGGTTGGTTCTAAGGTTAGAAAGAAATTCAGTTCTGGTTCCTTCAATCTCAAATTTGATTTCGTTAAGAAAGTTACTAAGAACAAGAAGATGGGTTCTGATTTTTCACCAACACCTCCAAGGGTCAAATGTGCATGGAATCCCATCATCAATAATAAGCAATGGGGCCGGGCATTGAAAGCTGCCTCAAAACAGTTCAAGAAATGGAAGGCGAAGAGAGACTCCATTGCACATGTGATGCTACAAAAGTCTTTCAAAGATGGAGATTTTTAAATAGAGTTATGTTTACAATGTTATTTTGATTTTGTTGATATTGGTATTGGTTCAATAAGCAACGTGCTTTACTTTCATAGTTTATTTCTTCT
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05536 Ps_contig_mira-and-tgicl-ass_franssen_38453
SEQUENCE=GATCCACGACACACATTCTGTTAACACTTGCGATCATCTATATTCACTCATCGAACTCCTTTTTCTCAAACACTATGTAACATGGCTTCTTCCTTCTTATTATTCCGGCGACACGCTACCAAAATCAACACAAACCTCACCTACTTCAACTGCCACGTGTGGACACGCGCCTTCTCAGAACAATCGAACAAAACAGAACGTATAAACAAAATTCTAATAGCAAACAGAGGCGAAATAGCTTGCAGGATCGCCAGAACCGCCAAGAGGCTCGGAATCAAAACCGTCGCCGTTTACAGCGACGCCGATAGAGACTCTCTCCACGTGGCGTCCTCTGATGAAGCCATACGAATCGGTCCTCCACCTCCTCGTCTCAGTTACCTCAACTCTTCGTCCATTATCGATGCTGCCTTGCGTTCCGGTGCTCAGGCTATCCACCCTGGATATGGATTCCTATCGGAAAGTGCTGATTTTGCACAACTATGTCATGATAAAGGTATTGCTTTCATTGGACCTCCGGCTTCTGCAATCCGAGATATGGGTGACAAGAGTGCATCGAAGAGAATAATGGGGGCTGCAGGGGTGCCACTTGTGCCTGGATATCATGGAGATGAACAAGATATTGATAAAATGAAGTTGGAGGCTGATCAGATTGGGTATCCAGTTCTCATAAAGCCAACCCATGGAGGAGGAGGAAAGGGTATGAGGATTGTGCACACTCCAGATGAATTTGTGGAATCATTCCTTGCAGCACAACGTGAAGCAGCTGCTTCTTTTGGTGTCAATACAATTTTGCTGGAGAAGTATATTACACAGCCGAGGCACATAGAAGTCCAAATATTTGGGGATAAGCATGGGAATGTTCTGCATTTGAATGAGAGAGATTGCAGTGTACAGAGAAGACACCAAAAAATAATTGAAGAAGCTCCAG
TARGET=682,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0874
PRIMER_LEFT_PENALTY=0.058146
PRIMER_RIGHT_PENALTY=0.029253
PRIMER_LEFT_SEQUENCE=GTGACAAGAGTGCATCGAAGAG
PRIMER_RIGHT_SEQUENCE=TTCCACAAATTCATCTGGAGTG
PRIMER_LEFT=538,22
PRIMER_RIGHT=734,22
PRIMER_LEFT_TM=60.058
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=0.1247
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.029253
PRIMER_LEFT_1_SEQUENCE=TCCTATCGGAAAGTGCTGATTT
PRIMER_RIGHT_1_SEQUENCE=TTCCACAAATTCATCTGGAGTG
PRIMER_LEFT_1=448,22
PRIMER_RIGHT_1=734,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=59.971
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.1247
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.029253
PRIMER_LEFT_2_SEQUENCE=TTCCTATCGGAAAGTGCTGATT
PRIMER_RIGHT_2_SEQUENCE=TTCCACAAATTCATCTGGAGTG
PRIMER_LEFT_2=447,22
PRIMER_RIGHT_2=734,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=59.971
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.1879
PRIMER_LEFT_3_PENALTY=0.158697
PRIMER_RIGHT_3_PENALTY=0.029253
PRIMER_LEFT_3_SEQUENCE=GGTATTGCTTTCATTGGACCTC
PRIMER_RIGHT_3_SEQUENCE=TTCCACAAATTCATCTGGAGTG
PRIMER_LEFT_3=492,22
PRIMER_RIGHT_3=734,22
PRIMER_LEFT_3_TM=59.841
PRIMER_RIGHT_3_TM=59.971
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.2370
PRIMER_LEFT_4_PENALTY=0.207756
PRIMER_RIGHT_4_PENALTY=0.029253
PRIMER_LEFT_4_SEQUENCE=AATCCGAGATATGGGTGACAAG
PRIMER_RIGHT_4_SEQUENCE=TTCCACAAATTCATCTGGAGTG
PRIMER_LEFT_4=524,22
PRIMER_RIGHT_4=734,22
PRIMER_LEFT_4_TM=60.208
PRIMER_RIGHT_4_TM=59.971
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_05537 Ps_contig_mira-and-tgicl-ass_franssen_38468
SEQUENCE=TTACGGAACTCCCAACTGTAAACCCGGACCTTGAACCTTTTCGTATATCCTCCAGCAAAGARTTCCAAGCCTCACGACCACACCGACGGTGCTTCGCCACATCTTCCTTCTTCTTCTTCTTCCACCACTGGAGACAAGGTCTTCGACGATGCTGTCAATCAGGTTGATCAGTTCCTTCGGGAAGCAATTCAAAACCCTCGTGAACGCCTTTCAGTTTTACGGATGGAGCAAGATGTTCTGAATTTTATTCAAGATCCTAATCAACAGCAGATTGAGTTTAATCAGCTTCCAACATCCTATTTGAGGTTGGCTGCACACCGGGTGGCTCAGCATTATTCATTGCAGTCGATGGTTTTACTGGATAATAATAGTCTACCGGATGGTTCTGGGTCAAGAATTATTGTTCGCAAAACTGCTGAATGTAAGGTTCCTATTATTCGCCTTGCGGACATCCCAGTGAA
TARGET=107,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05538 Ps_contig_mira-and-tgicl-ass_franssen_38483
SEQUENCE=AAGACAGTGGTATNACGCAGAGTACGCGGGACACAATTGCAATCCTAATTCCTAGAAAAATTACTTTTATTCTCTCTCTCTAACTTTCTCTCTCTAGAAATGTTAATGTCTTTCATCTTCTTATAACACAAACCCTTTTATCCTCTATTCATTCTCTCTTCAATATTTTCTTTTCTCTCACCAAAGCTCATCTGAGAGAAAAGAAAATAGGGTTTTCCGTTTTCTGAAGATGGGAAAGTGGGTGGAGATTATCGATCAAGGTGCGAGGATTGTTGCAAGGTCTTATTCCAACTGTCCTCAAACAGGTCGGAAGTTTTATCATCCTCCGCCTCATTCTGATATCGGCGACAATCATGCTCATGGAGGCGGCGCCGTGAACGGCGGCGGCGGCGGGAAATCCGGTTTTGGTTACGGAGGGAGTGGGTTTGATGTTATTCTTTATTCTGTTTGATTATGAGAAGTTTTGTTTTCTTTAATCGTTGTTATAGACGATTGTAGGGTTTTCTTTTATTAATTACTATGAATCTCGGATATTTTATTTGTTATTTAATTTATGTTAATTGTTTTTATTTAGTGATTCTCTGCGTGTTCTTGATGCGTTTACTTGATGAATCATTGACGTGTTTTTGGTAGTTTTTAAGTT
TARGET=71,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1016
PRIMER_LEFT_PENALTY=0.081801
PRIMER_RIGHT_PENALTY=0.019805
PRIMER_LEFT_SEQUENCE=GGGACACAATTGCAATCCTAAT
PRIMER_RIGHT_SEQUENCE=CATCTTCAGAAAACGGAAAACC
PRIMER_LEFT=27,22
PRIMER_RIGHT=231,22
PRIMER_LEFT_TM=60.082
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=205
PRIMER_PAIR_PENALTY_1=0.1016
PRIMER_LEFT_1_PENALTY=0.081801
PRIMER_RIGHT_1_PENALTY=0.019805
PRIMER_LEFT_1_SEQUENCE=GGGACACAATTGCAATCCTAAT
PRIMER_RIGHT_1_SEQUENCE=CCATCTTCAGAAAACGGAAAAC
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=60.082
PRIMER_RIGHT_1_TM=59.980
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=0.2155
PRIMER_LEFT_2_PENALTY=0.081801
PRIMER_RIGHT_2_PENALTY=0.133741
PRIMER_LEFT_2_SEQUENCE=GGGACACAATTGCAATCCTAAT
PRIMER_RIGHT_2_SEQUENCE=TCAGATGAGCTTTGGTGAGAGA
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=194,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=60.134
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.2155
PRIMER_LEFT_3_PENALTY=0.081801
PRIMER_RIGHT_3_PENALTY=0.133741
PRIMER_LEFT_3_SEQUENCE=GGGACACAATTGCAATCCTAAT
PRIMER_RIGHT_3_SEQUENCE=TCTCTCAGATGAGCTTTGGTGA
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=198,22
PRIMER_LEFT_3_TM=60.082
PRIMER_RIGHT_3_TM=60.134
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.2464
PRIMER_LEFT_4_PENALTY=0.081801
PRIMER_RIGHT_4_PENALTY=0.164639
PRIMER_LEFT_4_SEQUENCE=GGGACACAATTGCAATCCTAAT
PRIMER_RIGHT_4_SEQUENCE=GGATGATAAAACTTCCGACCTG
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=323,22
PRIMER_LEFT_4_TM=60.082
PRIMER_RIGHT_4_TM=59.835
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_05539 Ps_contig_mira-and-tgicl-ass_franssen_38483
SEQUENCE=AAGACAGTGGTATNACGCAGAGTACGCGGGACACAATTGCAATCCTAATTCCTAGAAAAATTACTTTTATTCTCTCTCTCTAACTTTCTCTCTCTAGAAATGTTAATGTCTTTCATCTTCTTATAACACAAACCCTTTTATCCTCTATTCATTCTCTCTTCAATATTTTCTTTTCTCTCACCAAAGCTCATCTGAGAGAAAAGAAAATAGGGTTTTCCGTTTTCTGAAGATGGGAAAGTGGGTGGAGATTATCGATCAAGGTGCGAGGATTGTTGCAAGGTCTTATTCCAACTGTCCTCAAACAGGTCGGAAGTTTTATCATCCTCCGCCTCATTCTGATATCGGCGACAATCATGCTCATGGAGGCGGCGCCGTGAACGGCGGCGGCGGCGGGAAATCCGGTTTTGGTTACGGAGGGAGTGGGTTTGATGTTATTCTTTATTCTGTTTGATTATGAGAAGTTTTGTTTTCTTTAATCGTTGTTATAGACGATTGTAGGGTTTTCTTTTATTAATTACTATGAATCTCGGATATTTTATTTGTTATTTAATTTATGTTAATTGTTTTTATTTAGTGATTCTCTGCGTGTTCTTGATGCGTTTACTTGATGAATCATTGACGTGTTTTTGGTAGTTTTTAAGTT
TARGET=379,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7828
PRIMER_LEFT_PENALTY=0.359050
PRIMER_RIGHT_PENALTY=0.423703
PRIMER_LEFT_SEQUENCE=TAGGGTTTTCCGTTTTCTGAAG
PRIMER_RIGHT_SEQUENCE=GAATAACATCAAACCCACTCCC
PRIMER_LEFT=207,22
PRIMER_RIGHT=436,22
PRIMER_LEFT_TM=59.641
PRIMER_RIGHT_TM=59.576
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.7923
PRIMER_LEFT_1_PENALTY=0.368624
PRIMER_RIGHT_1_PENALTY=0.423703
PRIMER_LEFT_1_SEQUENCE=CGTTTTCTGAAGATGGGAAAGT
PRIMER_RIGHT_1_SEQUENCE=GAATAACATCAAACCCACTCCC
PRIMER_LEFT_1=217,22
PRIMER_RIGHT_1=436,22
PRIMER_LEFT_1_TM=59.631
PRIMER_RIGHT_1_TM=59.576
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=220
PRIMER_PAIR_PENALTY_2=0.8082
PRIMER_LEFT_2_PENALTY=0.384515
PRIMER_RIGHT_2_PENALTY=0.423703
PRIMER_LEFT_2_SEQUENCE=TTCTCTCACCAAAGCTCATCTG
PRIMER_RIGHT_2_SEQUENCE=GAATAACATCAAACCCACTCCC
PRIMER_LEFT_2=172,22
PRIMER_RIGHT_2=436,22
PRIMER_LEFT_2_TM=59.615
PRIMER_RIGHT_2_TM=59.576
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.8082
PRIMER_LEFT_3_PENALTY=0.384515
PRIMER_RIGHT_3_PENALTY=0.423703
PRIMER_LEFT_3_SEQUENCE=CACCAAAGCTCATCTGAGAGAA
PRIMER_RIGHT_3_SEQUENCE=GAATAACATCAAACCCACTCCC
PRIMER_LEFT_3=178,22
PRIMER_RIGHT_3=436,22
PRIMER_LEFT_3_TM=59.615
PRIMER_RIGHT_3_TM=59.576
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.8184
PRIMER_LEFT_4_PENALTY=0.394672
PRIMER_RIGHT_4_PENALTY=0.423703
PRIMER_LEFT_4_SEQUENCE=ATTCCAACTGTCCTCAAACAGG
PRIMER_RIGHT_4_SEQUENCE=GAATAACATCAAACCCACTCCC
PRIMER_LEFT_4=284,22
PRIMER_RIGHT_4=436,22
PRIMER_LEFT_4_TM=60.395
PRIMER_RIGHT_4_TM=59.576
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=153
=
PRIMER_SEQUENCE_ID=SSR_05540 Ps_contig_mira-and-tgicl-ass_franssen_38490
SEQUENCE=ATTAGAAAACACAAAACCCTATTTGCAAAATCAATAGTATTGAAAGGGAAAAACACAATCAAGATTCCAACAAAAGGGGAAAGAATGAATGAATCACCAAATTATTGAAATTAGTGAAAATTGGTGAAATAATTGATCCAATTCCAGCAACACATGCAACAAGCAATGAAAAACATAGACTAGCATAAACCAATGCAACAAATGAACCAAATTTTTGACCTAA
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.0236
PRIMER_LEFT_PENALTY=0.666595
PRIMER_RIGHT_PENALTY=2.356982
PRIMER_LEFT_SEQUENCE=AGATTCCAACAAAAGGGGAAAG
PRIMER_RIGHT_SEQUENCE=TGGTTCATTTGTTGCATTGG
PRIMER_LEFT=61,22
PRIMER_RIGHT=208,20
PRIMER_LEFT_TM=60.667
PRIMER_RIGHT_TM=60.357
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=3.3862
PRIMER_LEFT_1_PENALTY=0.666595
PRIMER_RIGHT_1_PENALTY=2.719607
PRIMER_LEFT_1_SEQUENCE=AGATTCCAACAAAAGGGGAAAG
PRIMER_RIGHT_1_SEQUENCE=TTCATTGCTTGTTGCATGTG
PRIMER_LEFT_1=61,22
PRIMER_RIGHT_1=169,20
PRIMER_LEFT_1_TM=60.667
PRIMER_RIGHT_1_TM=59.280
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=3.5327
PRIMER_LEFT_2_PENALTY=1.377001
PRIMER_RIGHT_2_PENALTY=2.155705
PRIMER_LEFT_2_SEQUENCE=ATCAAGATTCCAACAAAAGGGG
PRIMER_RIGHT_2_SEQUENCE=CATTGCTTGTTGCATGTGTTG
PRIMER_LEFT_2=57,22
PRIMER_RIGHT_2=167,21
PRIMER_LEFT_2_TM=61.377
PRIMER_RIGHT_2_TM=61.156
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=111
PRIMER_PAIR_PENALTY_3=3.5505
PRIMER_LEFT_3_PENALTY=0.666595
PRIMER_RIGHT_3_PENALTY=2.883954
PRIMER_LEFT_3_SEQUENCE=AGATTCCAACAAAAGGGGAAAG
PRIMER_RIGHT_3_SEQUENCE=ATTGCTTGTTGCATGTGTTGCT
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=166,22
PRIMER_LEFT_3_TM=60.667
PRIMER_RIGHT_3_TM=62.884
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=3.5563
PRIMER_LEFT_4_PENALTY=1.199358
PRIMER_RIGHT_4_PENALTY=2.356982
PRIMER_LEFT_4_SEQUENCE=GATTCCAACAAAAGGGGAAAG
PRIMER_RIGHT_4_SEQUENCE=TGGTTCATTTGTTGCATTGG
PRIMER_LEFT_4=62,21
PRIMER_RIGHT_4=208,20
PRIMER_LEFT_4_TM=59.801
PRIMER_RIGHT_4_TM=60.357
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_05541 Ps_contig_mira-and-tgicl-ass_franssen_38494
SEQUENCE=ATTGCACATTTTAAGAACATTACATTTTCAGTAACCAAACTAGTTCCTCTTTTACTATATCCATAACAAATGGCTTTTTCTAAGCTTCTACTTTCACCTCTTTTGCTATCTTTTCTCATTCTCCATCTTGTTCATGCTCAACAGGAACCACTGGTACATTCCCAAACTCAGGTTTCGCTTCAGCAGCAGATAGACTGCAACGGAGCATGTGCTGCGAGATGCCGTTTAGCATCTCGGCAGCACCTCTGCCATCGGGCATGTGGAACTTGCTGCAGGCGCTGCAACTGCGTGCCACCGGGAACTTCCGGTGGCCAAGAAGTGTGTCCATGCTATGCCAACCAAACCACCCACGGAGGCAGACGCAAGTGCCCTTGAATAAGTTGAATTAATCACTTCCATAACCAACTTATGCAATGCAACTTATCTATGTGTGTGTGGAGCAAAGACTTGTAGCTTTGTGTAAAATAAAATCTGGTTCTAATTCATGTTGTATTTTATGATAATTTGCAGTGTGTGTTTATGAATGTAATTTATAATAATTGCATTGCGATCATATTATTATTGTGCTTTATCTATTACATCGGTTATGAACTTATGAGTGTTATCTATCAGTATATTGTTTATTGTAATTGTATACTACAATAAAAATTTACAAATTAAAACTTTTTGTAATTTTTAAATTTAAAA
TARGET=428,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05542 Ps_contig_mira-and-tgicl-ass_franssen_38500
SEQUENCE=GGATGCTGGATTTGGTAATGTTTTTAGGGTTTTCTTTTGGGATTGTTTTGATGATGAGTTTCAGACTGAGAAAAGAAGATATAAAATTTTGGGTTTCAGAAGCTAAGAACTTGTTCTCCAGTTGCACAAATTGCATTGAGGAATAGAAATAGAAAATGGAGATTGTCAAAATGAAAGCTTTTTCTTTCTTTCTTCCTTCACACTGTTGTTTGATTCAATTCAGCCACCAAAAGATTGTTTGTGTGGTTGCTGATTCTTGTAATGCTTAATGATGCTTGGAATTCAATGTAACAATGATAAAATGCAAGTCATTGAATGATATAATGAGTGATG
TARGET=181,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1558
PRIMER_LEFT_PENALTY=0.081801
PRIMER_RIGHT_PENALTY=0.073989
PRIMER_LEFT_SEQUENCE=GGATGCTGGATTTGGTAATGTT
PRIMER_RIGHT_SEQUENCE=CAGCAACCACACAAACAATCTT
PRIMER_LEFT=0,22
PRIMER_RIGHT=251,22
PRIMER_LEFT_TM=60.082
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.1773
PRIMER_LEFT_1_PENALTY=0.081801
PRIMER_RIGHT_1_PENALTY=0.095523
PRIMER_LEFT_1_SEQUENCE=GGATGCTGGATTTGGTAATGTT
PRIMER_RIGHT_1_SEQUENCE=ATCAGCAACCACACAAACAATC
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=253,22
PRIMER_LEFT_1_TM=60.082
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=254
PRIMER_PAIR_PENALTY_2=0.2548
PRIMER_LEFT_2_PENALTY=0.180765
PRIMER_RIGHT_2_PENALTY=0.073989
PRIMER_LEFT_2_SEQUENCE=ACTTGTTCTCCAGTTGCACAAA
PRIMER_RIGHT_2_SEQUENCE=CAGCAACCACACAAACAATCTT
PRIMER_LEFT_2=108,22
PRIMER_RIGHT_2=251,22
PRIMER_LEFT_2_TM=59.819
PRIMER_RIGHT_2_TM=60.074
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=0.2763
PRIMER_LEFT_3_PENALTY=0.180765
PRIMER_RIGHT_3_PENALTY=0.095523
PRIMER_LEFT_3_SEQUENCE=ACTTGTTCTCCAGTTGCACAAA
PRIMER_RIGHT_3_SEQUENCE=ATCAGCAACCACACAAACAATC
PRIMER_LEFT_3=108,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=59.819
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.3189
PRIMER_LEFT_4_PENALTY=0.180765
PRIMER_RIGHT_4_PENALTY=0.138114
PRIMER_LEFT_4_SEQUENCE=ACTTGTTCTCCAGTTGCACAAA
PRIMER_RIGHT_4_SEQUENCE=GCATTACAAGAATCAGCAACCA
PRIMER_LEFT_4=108,22
PRIMER_RIGHT_4=264,22
PRIMER_LEFT_4_TM=59.819
PRIMER_RIGHT_4_TM=60.138
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_05543 Ps_contig_mira-and-tgicl-ass_franssen_38501
SEQUENCE=AATTNGAAGNAGTTGCAGTAGTACTGATTTCTGAAGAGACCGAGTAGTACTGATTTCCACTTCCAATTCCAAGTGCGAACTCGAAGCAATGGATAGAGAATGGGGTTCAAAACCGGGAAGCGGCGGAGCCGCGACGGCGCAAAACGAAGCAATAGACCGCCGCGAGCGTCTCCGACGACTAGCACTCGAAACAATAGATCTAGCCAAAGATCCATATTTCATGCGCAATCATCTCGGTAGCTACGAGTGCAAACTCTGTTTAACTCTCCACAACAACGAAGGAAACTACCTCGCACATACTCAAGGCAAACGCCATCAAACCAATCTCGCTAAGCGTGCTGCTCGTGAAGCTAAAGATGCTCCCACTCAACCTCAACCTCATAAGCGGAAGCTTAACCTTAAGAAAACTGTTAAGATTGGTCGTCCGGGTTACCGTGTAACTAAACAATTTGATCCTGACACTAAGCAAAGATCACTCCTTTTTCAGATTGAATATCCTGAGATTGAAGACCTTGCAAAGCCTAGGCATCGATTTATGTCCTCCTATGAGCAGAGGGTGCAACCATTTGATAAAAGATATCAGTATCTCTTGTTTGCAGCTGAACCATATGAAACAATTTCTTTCAAGGTCCCTAGCACAGAGATTGACAAGTCCACGCCAAAGTTTTTCTCGCATTGGGACCCAGATTCCAAGATGTTTACGCTGCAGTTGTATTTTAAAATCAAACCACTAGAGGTAACCAAACCACAGCCACCTGCATCTACTCCCAATGGCACCACAGCACCCGGTGTTCCTCCAAGGCCAATGCCCCCAGCATCACAAGCTCCATTACCACCACCACCTCCTCCCCAAGGGCTGCCTCCTGGCGCACCTTTGGGAAATCCTCCTCGAGCCCCTCCACCTCCAATGTCAGGATCAATGCCTCCACCACCTCCTATGTCTGCAAATGGTCCAAGACCTGTACCCGGTGCAATGCCGCCTCTTCCACCTCCAGCTCCCCTTGGCGCTCGACCTCCATCAATGCCACCTCCGCAAGGTTTTCCAGGCCAACAAATGTAGAGCTAGGGCATCTGTTTGCCTCCGCCAATTGCACATGACCTACCCAGTCCGCATGGCAGTATGCTGATCAGTATCGTGTGTTAAATAGTTGTAGTTGGACGCGCCTTATCCTCTGGGTCTCAGTATATTTGTTATGTCAGATGCTAGTTTTCATAAACAATACCCCAAATTGGCAATACATTGTTTATTGCATCTCATTTTTGCAACTTACAAATTTATACATTTGATTTGCAGTATCAAGTTGTTTGGTATTTAATGTTTATGTTGACCATCTTTCACTAAACGTGGTTCTCCAATCCCAAGGTATAGATGGAAATGTTTTGGTTTGTACCGTAGCACAAGAACATCCATAGGCATCCGTGATAATTAATGTAATAACATTGTTGCTTTATCATTATTTGCTTACTT
TARGET=832,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5128
PRIMER_LEFT_PENALTY=0.404566
PRIMER_RIGHT_PENALTY=0.108206
PRIMER_LEFT_SEQUENCE=TAGAGGTAACCAAACCACAGCC
PRIMER_RIGHT_SEQUENCE=ACCATTTGCAGACATAGGAGGT
PRIMER_LEFT=731,22
PRIMER_RIGHT=951,22
PRIMER_LEFT_TM=60.405
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.7728
PRIMER_LEFT_1_PENALTY=0.404566
PRIMER_RIGHT_1_PENALTY=0.368190
PRIMER_LEFT_1_SEQUENCE=TAGAGGTAACCAAACCACAGCC
PRIMER_RIGHT_1_SEQUENCE=CTTGGACCATTTGCAGACATAG
PRIMER_LEFT_1=731,22
PRIMER_RIGHT_1=956,22
PRIMER_LEFT_1_TM=60.405
PRIMER_RIGHT_1_TM=59.632
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=226
PRIMER_PAIR_PENALTY_2=0.8205
PRIMER_LEFT_2_PENALTY=0.712323
PRIMER_RIGHT_2_PENALTY=0.108206
PRIMER_LEFT_2_SEQUENCE=CCCAGATTCCAAGATGTTTACG
PRIMER_RIGHT_2_SEQUENCE=ATTTGCAGACATAGGAGGTGGT
PRIMER_LEFT_2=681,22
PRIMER_RIGHT_2=948,22
PRIMER_LEFT_2_TM=60.712
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.8205
PRIMER_LEFT_3_PENALTY=0.712323
PRIMER_RIGHT_3_PENALTY=0.108206
PRIMER_LEFT_3_SEQUENCE=CCCAGATTCCAAGATGTTTACG
PRIMER_RIGHT_3_SEQUENCE=ACCATTTGCAGACATAGGAGGT
PRIMER_LEFT_3=681,22
PRIMER_RIGHT_3=951,22
PRIMER_LEFT_3_TM=60.712
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=271
PRIMER_PAIR_PENALTY_4=0.9064
PRIMER_LEFT_4_PENALTY=0.404566
PRIMER_RIGHT_4_PENALTY=0.501869
PRIMER_LEFT_4_SEQUENCE=TAGAGGTAACCAAACCACAGCC
PRIMER_RIGHT_4_SEQUENCE=TCTTGGACCATTTGCAGACATA
PRIMER_LEFT_4=731,22
PRIMER_RIGHT_4=957,22
PRIMER_LEFT_4_TM=60.405
PRIMER_RIGHT_4_TM=60.502
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_05544 Ps_contig_mira-and-tgicl-ass_franssen_38502
SEQUENCE=TGTTTCTTCTGTYNRRNCATCCATAACTCATTTTCTCAAAAAATAAAAATCATCAATAATTCATCATTCACCTAAAAACATCACAAAATACCCAAAATATCTGAAATTTCCGATCACCGGAGTTCAGATCCCGAAACCCACACAATGACGACGACGACGACGTTTCCATCGCCGCTAAAAGCCATAACCCTAACCCACATCCGTTACGGAAGAGGCGACAAATTAGGTCATTTCTTAGCATGGATATCACTCGTCCCCGTCTTCATCAGCTTCGGCGGTTTCATCTCCCACTTCATCTTCCGYCGCGAAATTCAAGGAATCTTCTTCTTCCTCGGCTTAATCGTTTCCCAATTCATCAACGAGTTCATCAAAACCACCGTCCAACAAGCTCGG
TARGET=147,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05545 Ps_contig_mira-and-tgicl-ass_franssen_38514
SEQUENCE=AAAGAAAAAAGAAAAGAAAAGAAGAAGAAAATAAAGAGTCTCATAGAGTTAGACTGTTCAGCAAGAGACTGAAAAATGACGTCGTTTTGTGGTGCGACGTGCTTCCAGATTCCGGTGATCAACGGCGGCGTACTACGCAACCGCGCTTCATTGACCGGCGGCGCATCCTTCTGCAATACATCGAAGCGCTCTTTGTCGTTCACATGCTTGAGCGTTTCTTCTGATGCCAGAATAGGAGTTAAGGAAGCTGTTGAAACGGAAAAAGCACCTGCAGCATTGGGACCATATTCCCAAGCAAACAAAGTTAACAATCTTCTTTTTGTGTCTGGTGTTCTTGGCCTTGTTCCTGAGACGGGAAAGTTCGTCTCTGATAATGTTGATGATCAAACCGAACAGCTTCTCAAAAACATGGGGGAAATCCTTAAAGCAGGTGGTGCAAGCTACTCATCAGTTGTTAAAACAACGATTCTGTTAGCTGACTTGAAGGACTTTAAGACAGTCAATGAGATTTATGCTAAATATTTCCCTGCACCTTTCCCCGCTCGTGCGACTTATCAAGTAGCTGCATTGCCATTGGACGCCAAGATTGAAATCGAGTGCATTGCAACACTCTAAGCGCTAATTTCAGTAGTATATCTATTTAGCTTCTTCAGCAAACACAGGTCAATAATACTGCAGGAGGGACATGTAGTTTAGTATTTTTACTTTAGTTTGTTTGCCATGGATGCTGAAGCTATAGTTTTGCATTACATAAGGTTGCCATGAATAAAGTTGAAACATTGTTTGTTTCAAATAAGATCAGTTCTAATATATGTAATCTTTATAGTTGGTACAAAAACNTGCATGATTTAACAAG
TARGET=7,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05546 Ps_contig_mira-and-tgicl-ass_franssen_38517
SEQUENCE=AGTACGCGAAAGAAAGAAAAAGGATTATGGATGACAAGTGAGAACAACCCAATATTTTATATCACACACAAAACCTCGTGTATTTGATATTTACGTTAAAAATTAGATTTTGAATTTGTRGTTTTCAGAGAG
TARGET=8,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05547 Ps_contig_mira-and-tgicl-ass_franssen_38539
SEQUENCE=AATCTTGTCTTACTTGTCATTAGTCTTTTCCTCTAAAAGAGGAAAAAGTGAGGAGAGGATNCAAATCCGAAATAAAATTCTGAGAATAATTTGCAATAGAAAGAGAATGGTGAAACCAAATGAAGTAGAAGGAGTAGCCGTACCTGAATCAACAATAGAACTACTCAACCGTACTTTATACGACATTGAGCAACTCGAACCCCAATTGCCCCAATTTCTCTCCCTCTCAGACCCTGATTACCTCTCCCAACTCCCACTCCTTCGACGTGCTCACTCTCTCATCTCCCTCGCAAAACTCACTTCAACTCTCTTCTCATTGAAGTTGAAGTGTAGAGGAATTAACCCAAATGACCACCCTTTCAAATTTGAGCTTGATAGGGTTAGTGTGTGCCAAAATAGACTGGAACGCCTTCCCAATTTCAGTGAAGCTGAGTGGCAGGATATGGTGGAGGAAAATCTGAACTATCATGAGCAGACTGGTCAAAAGAGGAAGTATCCATCATCTGAAGAACAACCTGATCAATATGATTCCAAGGAGTTTCTGGAGAAATCAACAGGGGAGCTTCTTGATGGTGGTAATAGTGGAAGTAGTATTATTAAGAAATCAATAATAGTTGACTTATCCGATGATGATGATGACGACGACGATGATGATGAGTATATGTGAGTGAACAGCAAGGAGCGCAGTGTTCATGGGAGGGAATGATTTTGATGTGTTTCTGCGTCATAGTTGCTTTTCCATGCTTTTGAATGACTCATCCATTTTTGGCAATAGAAATCGAGGGCACGGCAACATGTCTCTAAGCAGCGATATATCAAAGTTGTAATGCATGTATAGATTCAATCTTGATGTGTGACAGACATGCTATAAATTGTTGTTTGGAAGGATAAATTGTTTCTGCGTCGTAGGATACTAAGATTTTAACTCAACTATGTACATGTATGGATTTAATGTTAGATGATGATGTTCTTGTTTGATTACAAA
TARGET=626,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0759
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.052366
PRIMER_LEFT_SEQUENCE=GTTTCTGGAGAAATCAACAGGG
PRIMER_RIGHT_SEQUENCE=AAGCAACTATGACGCAGAAACA
PRIMER_LEFT=537,22
PRIMER_RIGHT=735,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.948
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.0838
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.060196
PRIMER_LEFT_1_SEQUENCE=GTTTCTGGAGAAATCAACAGGG
PRIMER_RIGHT_1_SEQUENCE=ACACATCAAAATCATTCCCTCC
PRIMER_LEFT_1=537,22
PRIMER_RIGHT_1=716,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.060
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.3864
PRIMER_LEFT_2_PENALTY=0.334070
PRIMER_RIGHT_2_PENALTY=0.052366
PRIMER_LEFT_2_SEQUENCE=CAGGATATGGTGGAGGAAAATC
PRIMER_RIGHT_2_SEQUENCE=AAGCAACTATGACGCAGAAACA
PRIMER_LEFT_2=436,22
PRIMER_RIGHT_2=735,22
PRIMER_LEFT_2_TM=59.666
PRIMER_RIGHT_2_TM=59.948
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.3864
PRIMER_LEFT_3_PENALTY=0.334070
PRIMER_RIGHT_3_PENALTY=0.052366
PRIMER_LEFT_3_SEQUENCE=GGATATGGTGGAGGAAAATCTG
PRIMER_RIGHT_3_SEQUENCE=AAGCAACTATGACGCAGAAACA
PRIMER_LEFT_3=438,22
PRIMER_RIGHT_3=735,22
PRIMER_LEFT_3_TM=59.666
PRIMER_RIGHT_3_TM=59.948
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.3943
PRIMER_LEFT_4_PENALTY=0.334070
PRIMER_RIGHT_4_PENALTY=0.060196
PRIMER_LEFT_4_SEQUENCE=GGATATGGTGGAGGAAAATCTG
PRIMER_RIGHT_4_SEQUENCE=ACACATCAAAATCATTCCCTCC
PRIMER_LEFT_4=438,22
PRIMER_RIGHT_4=716,22
PRIMER_LEFT_4_TM=59.666
PRIMER_RIGHT_4_TM=60.060
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=279
=
PRIMER_SEQUENCE_ID=SSR_05548 Ps_contig_mira-and-tgicl-ass_franssen_38550
SEQUENCE=ATCTCCTTATAAACCTTAATTATCAAGTTTATCATCCCACCTTAGAAGCAAAATTGTGCACCTCCACATGATATACAATCTAGTTTTCTGCTCTTTGAACTGCACTGAACACCGTCGCATAAGGTGATGAGGCTTTGGAATTCTCATTGATAATGATTCTCTTGCCATATTTACTACTACTACTAGTATTATACAAAATCCAAATCTTTTATGATGCTGCAGCCATAGCTCTTATAGTAGAACTTATGATNATCCCCGC
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2801
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.245968
PRIMER_LEFT_SEQUENCE=ATCATCCCACCTTAGAAGCAAA
PRIMER_RIGHT_SEQUENCE=TATGGCTGCAGCATCATAAAAG
PRIMER_LEFT=30,22
PRIMER_RIGHT=226,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.246
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=197
PRIMER_PAIR_PENALTY_1=0.2801
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.245968
PRIMER_LEFT_1_SEQUENCE=ATCATCCCACCTTAGAAGCAAA
PRIMER_RIGHT_1_SEQUENCE=CTATGGCTGCAGCATCATAAAA
PRIMER_LEFT_1=30,22
PRIMER_RIGHT_1=227,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.246
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.2801
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.245968
PRIMER_LEFT_2_SEQUENCE=TAAGGTGATGAGGCTTTGGAAT
PRIMER_RIGHT_2_SEQUENCE=TATGGCTGCAGCATCATAAAAG
PRIMER_LEFT_2=119,22
PRIMER_RIGHT_2=226,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=60.246
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.2801
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.245968
PRIMER_LEFT_3_SEQUENCE=ATAAGGTGATGAGGCTTTGGAA
PRIMER_RIGHT_3_SEQUENCE=TATGGCTGCAGCATCATAAAAG
PRIMER_LEFT_3=118,22
PRIMER_RIGHT_3=226,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=60.246
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=0.2801
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.245968
PRIMER_LEFT_4_SEQUENCE=ATAAGGTGATGAGGCTTTGGAA
PRIMER_RIGHT_4_SEQUENCE=CTATGGCTGCAGCATCATAAAA
PRIMER_LEFT_4=118,22
PRIMER_RIGHT_4=227,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=60.246
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_05549 Ps_contig_mira-and-tgicl-ass_franssen_38556
SEQUENCE=AATCAATCATTCATTCATTATAGTCATACTTCAATATTTAATTCAAATGCCTTAACACGCTCTTCCCCTTTCCCCTCATTTTCTGCTCAGGGAATTGGATGGGAAGTCCTTTTTCTTTTGGGTTTTTTACTGCTGCTGCTGATGTTGTTCCGAGCATAGAAATTCAATGGYTGGCGTCTGTTTTCGCCGGTATCATCTTCAGTTTTATTGTTTATAGATTGACTGCAACTTTTAGTTCTCGGTTGTYYGNGGGATACAGTAAA
TARGET=7,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05550 Ps_contig_mira-and-tgicl-ass_franssen_38556
SEQUENCE=AATCAATCATTCATTCATTATAGTCATACTTCAATATTTAATTCAAATGCCTTAACACGCTCTTCCCCTTTCCCCTCATTTTCTGCTCAGGGAATTGGATGGGAAGTCCTTTTTCTTTTGGGTTTTTTACTGCTGCTGCTGATGTTGTTCCGAGCATAGAAATTCAATGGYTGGCGTCTGTTTTCGCCGGTATCATCTTCAGTTTTATTGTTTATAGATTGACTGCAACTTTTAGTTCTCGGTTGTYYGNGGGATACAGTAAA
TARGET=130,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05551 Ps_contig_mira-and-tgicl-ass_franssen_38561
SEQUENCE=AATAACTAACATAACACGTTGACTAATTTTGCTAGTTGCGCTGTCCCATTAGACAAATGCGAAACNCTATTGGTTTTCTTATAACTAATATTCATTATTTATTACCACTCCCNAACAACAAACTTATTTTATCTCTCACAACGTTATCAACACCACTTTCATTTGCATCATCCTCAGTTTCATTTCACTCCCAAATAAGTTATATAACCCAATCTCCCAAAACACACACAAACACATCATTATGCAGGTCAACGAAAAAAATGAGGAACCGTTATCCTCTTATCGGTGAAAAACGTTGGTCGGTAGTTTTGTTGGTCATTTTGGGAATAATCGGAGCATTGGTTTTCACAACAACCATACTCCAAACCTCCAACAACACCTTGTTTTGTTCACTCACCCGAACCTCGAACCAACAATCCAACCCTACACCGATCCAGCTTCGTGCCATCCTTCACTACGCAACCTCCCACGTTGTCCCTCAACAATCACTCGAAGAGATCAGCATAACCTTAAACGTCATCAAATCCCTAAACCGTCCCTTCAACTTCCTCGTATTCGGTCTCGGCCACGATTCCCTCATGTGGGCCGGGTTCAACCCACATGGAACCACGCTTTTCCTCGAAGAAGATCCGAAATGGGTTCAAACGGTTCTCAAAGACTCGCCGGAGCTTCGAGCTATTACCGTTCCCTACCGTACACAGCTTCAAGAAGCCGACGAGCTTCTCAAAACGTACCGATCTGAGCCGGCTTGTTCTCCGGCTAAAGCCACGCTGAAAGGCAACGAAAAATGTAAACTAGCGCTTCATAATCTCCCCGACGAGGTTTACGATACCGACTGGGATTTGATAATGATCGACGCGCCGAGAGGGTATTTCGCGGAGGCGCCGGGTCGTATGGCGGCGATATTTTCGATGAACGTGATGGCTAGAAATAGAAAAGGCTCCGATGTGACACACGTGTTTTTGCACGATGTAGATCGGAAGGTGGAGAAGGCTTACGCCGAAGAGTTTCTTTGTAAGAAAAACAAGGTGGATGGTGTGGGAAGACTCTGGCACTTCAAAATTCCTGCAATTGGAAACAATGACACTCGTCATGATTCCAATGATTCAACTTTCTGTTAAGTTTCAATGGCTTCGGATTTTTCAACGCTAGTTTTTGTCTTCCCACTAGTCGCGGAGGGTTTTTTGTTTTTCTCTATCTTATTTTATTTTATTTTTCTTGACCATAGAGTTTGGATCTAAAACATGTGGCGGTGATAGAAACACCATTGTAGTCATTCTAGTGAATAGTACTACTATTACTACTACGACTTTACTACTAGTTTTAATTTAAATTTGTTTTTCTCTAANACCG
TARGET=1200,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4147
PRIMER_LEFT_PENALTY=0.038361
PRIMER_RIGHT_PENALTY=0.376347
PRIMER_LEFT_SEQUENCE=ATGTAGATCGGAAGGTGGAGAA
PRIMER_RIGHT_SEQUENCE=TATCACCGCCACATGTTTTAGA
PRIMER_LEFT=969,22
PRIMER_RIGHT=1257,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=60.376
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.4147
PRIMER_LEFT_1_PENALTY=0.038361
PRIMER_RIGHT_1_PENALTY=0.376347
PRIMER_LEFT_1_SEQUENCE=ATGTAGATCGGAAGGTGGAGAA
PRIMER_RIGHT_1_SEQUENCE=TCTATCACCGCCACATGTTTTA
PRIMER_LEFT_1=969,22
PRIMER_RIGHT_1=1259,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=60.376
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=291
PRIMER_PAIR_PENALTY_2=0.5025
PRIMER_LEFT_2_PENALTY=0.038361
PRIMER_RIGHT_2_PENALTY=0.464167
PRIMER_LEFT_2_SEQUENCE=ATGTAGATCGGAAGGTGGAGAA
PRIMER_RIGHT_2_SEQUENCE=CTATCACCGCCACATGTTTTAG
PRIMER_LEFT_2=969,22
PRIMER_RIGHT_2=1258,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=59.536
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.5386
PRIMER_LEFT_3_PENALTY=0.162217
PRIMER_RIGHT_3_PENALTY=0.376347
PRIMER_LEFT_3_SEQUENCE=AACGCTAGTTTTTGTCTTCCCA
PRIMER_RIGHT_3_SEQUENCE=TCTATCACCGCCACATGTTTTA
PRIMER_LEFT_3=1144,22
PRIMER_RIGHT_3=1259,22
PRIMER_LEFT_3_TM=60.162
PRIMER_RIGHT_3_TM=60.376
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=116
PRIMER_PAIR_PENALTY_4=0.5386
PRIMER_LEFT_4_PENALTY=0.162217
PRIMER_RIGHT_4_PENALTY=0.376347
PRIMER_LEFT_4_SEQUENCE=CAACGCTAGTTTTTGTCTTCCC
PRIMER_RIGHT_4_SEQUENCE=TATCACCGCCACATGTTTTAGA
PRIMER_LEFT_4=1143,22
PRIMER_RIGHT_4=1257,22
PRIMER_LEFT_4_TM=60.162
PRIMER_RIGHT_4_TM=60.376
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_05552 Ps_contig_mira-and-tgicl-ass_franssen_38568
SEQUENCE=ACTCTCTCTTTTTCTCTTTCCTTTCTTTTCTTTTCTCTCAATCACACAACAAACAAACAAACAAACAGAACGTAACTAACTAACTCTCTTTATCCGATTCTTTCTTCTCTCTCTCTCTCTCAGATCTGGTTCTATCTTTTCTCTATTTCGATCTCTCATCGGATTTTCGGTTTAGATAATCGATTATTTCACCTGATGATGACGAAGAATAATGAAGGAAAAGTGGGAGATAAGAGCAGGATTGTTCCTGTGTCAATCATATTTGTGGTGCTTTGTGGTCTTTCTTTTTATTTAGGTGTACTATTTGGTTCTGAGAATGATAAATTTGTATCGATTATCAGTCAGAGATCCGTTGATTTCCGCAAGGAATCATCGTCGATAAGTTCTTTGAAAAATAACTCTACCAGTTTTCCTGAATGTGATATTGATTATCAAGATTTCACTCCATGTACTGACCCAAGGAGATGGAAGAAGTATGGATCTTATCGATTCATTATGTTAGAGCGTCATTGCCCTCCGGTTTTGAGAGGAAAGAATGCTTGGTTCCCTCCCCCAGATGGATACAAACCTCCAATTAGATGGCCAAAAGAGCAAAGA
TARGET=22,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05553 Ps_contig_mira-and-tgicl-ass_franssen_38568
SEQUENCE=ACTCTCTCTTTTTCTCTTTCCTTTCTTTTCTTTTCTCTCAATCACACAACAAACAAACAAACAAACAGAACGTAACTAACTAACTCTCTTTATCCGATTCTTTCTTCTCTCTCTCTCTCTCAGATCTGGTTCTATCTTTTCTCTATTTCGATCTCTCATCGGATTTTCGGTTTAGATAATCGATTATTTCACCTGATGATGACGAAGAATAATGAAGGAAAAGTGGGAGATAAGAGCAGGATTGTTCCTGTGTCAATCATATTTGTGGTGCTTTGTGGTCTTTCTTTTTATTTAGGTGTACTATTTGGTTCTGAGAATGATAAATTTGTATCGATTATCAGTCAGAGATCCGTTGATTTCCGCAAGGAATCATCGTCGATAAGTTCTTTGAAAAATAACTCTACCAGTTTTCCTGAATGTGATATTGATTATCAAGATTTCACTCCATGTACTGACCCAAGGAGATGGAAGAAGTATGGATCTTATCGATTCATTATGTTAGAGCGTCATTGCCCTCCGGTTTTGAGAGGAAAGAATGCTTGGTTCCCTCCCCCAGATGGATACAAACCTCCAATTAGATGGCCAAAAGAGCAAAGA
TARGET=48,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05554 Ps_contig_mira-and-tgicl-ass_franssen_38568
SEQUENCE=ACTCTCTCTTTTTCTCTTTCCTTTCTTTTCTTTTCTCTCAATCACACAACAAACAAACAAACAAACAGAACGTAACTAACTAACTCTCTTTATCCGATTCTTTCTTCTCTCTCTCTCTCTCAGATCTGGTTCTATCTTTTCTCTATTTCGATCTCTCATCGGATTTTCGGTTTAGATAATCGATTATTTCACCTGATGATGACGAAGAATAATGAAGGAAAAGTGGGAGATAAGAGCAGGATTGTTCCTGTGTCAATCATATTTGTGGTGCTTTGTGGTCTTTCTTTTTATTTAGGTGTACTATTTGGTTCTGAGAATGATAAATTTGTATCGATTATCAGTCAGAGATCCGTTGATTTCCGCAAGGAATCATCGTCGATAAGTTCTTTGAAAAATAACTCTACCAGTTTTCCTGAATGTGATATTGATTATCAAGATTTCACTCCATGTACTGACCCAAGGAGATGGAAGAAGTATGGATCTTATCGATTCATTATGTTAGAGCGTCATTGCCCTCCGGTTTTGAGAGGAAAGAATGCTTGGTTCCCTCCCCCAGATGGATACAAACCTCCAATTAGATGGCCAAAAGAGCAAAGA
TARGET=73,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05555 Ps_contig_mira-and-tgicl-ass_franssen_38568
SEQUENCE=ACTCTCTCTTTTTCTCTTTCCTTTCTTTTCTTTTCTCTCAATCACACAACAAACAAACAAACAAACAGAACGTAACTAACTAACTCTCTTTATCCGATTCTTTCTTCTCTCTCTCTCTCTCAGATCTGGTTCTATCTTTTCTCTATTTCGATCTCTCATCGGATTTTCGGTTTAGATAATCGATTATTTCACCTGATGATGACGAAGAATAATGAAGGAAAAGTGGGAGATAAGAGCAGGATTGTTCCTGTGTCAATCATATTTGTGGTGCTTTGTGGTCTTTCTTTTTATTTAGGTGTACTATTTGGTTCTGAGAATGATAAATTTGTATCGATTATCAGTCAGAGATCCGTTGATTTCCGCAAGGAATCATCGTCGATAAGTTCTTTGAAAAATAACTCTACCAGTTTTCCTGAATGTGATATTGATTATCAAGATTTCACTCCATGTACTGACCCAAGGAGATGGAAGAAGTATGGATCTTATCGATTCATTATGTTAGAGCGTCATTGCCCTCCGGTTTTGAGAGGAAAGAATGCTTGGTTCCCTCCCCCAGATGGATACAAACCTCCAATTAGATGGCCAAAAGAGCAAAGA
TARGET=106,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05556 Ps_contig_mira-and-tgicl-ass_franssen_38569
SEQUENCE=TGACCCCCTTTCGTCGTGAAGACGAAGAACCCCGCATGGGTTCATGTCATCATGGAACACAAGCACCTGATTAAGCGGCAACTTGTAATAAGCAGCCAGTGGTCCTGCCGATTCACCACTGAGATTCATGTAAGTTTGAGGTTTCGCAGGAAAACTGGAACTTCATCAACAAAACCTTTTCCAAAAATGGCTTTGCAATGAACCGTATTCATGGCAATCCCCTGTGATTCGGCAAATGCATCAATCATCTCAAAGCCAACATTGTGTCGAGTTCCTTTGTACTTGTCGCCAGGGTTACCCAAACCAACAAAAAGCCAAGGGCGGGGAGCGACGGTGCAAAAGCAACGCTTGGTCAATGTACTGAGCATATCCTCCACTGAAGTGGAACAGTGAGAAGAAGAAGAAAATTGAAGATTCTTCGATTTAGTTACTGGTGGTGTGTGAGCGGAGATAATTAAGGAGGGGATTAGATTAATTGAATATATCGGTGATTAGTGGTGGTGTGTTTAAAGTTGGTGGAGGCGGAGGTGGAGGCGCTCGCCGCGACACAGCTAACTCGGTTTTCCTATCGGAAGTTTAACCGGTTCGGTCCCGGTTTCATAGACTCGGGTTTGGTATGTGCATCTATATGGGTCGTAAATTCGTAATCAAACTAGACACTCCGATTCGT
TARGET=393,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2431
PRIMER_LEFT_PENALTY=0.206859
PRIMER_RIGHT_PENALTY=0.036271
PRIMER_LEFT_SEQUENCE=GTTACCCAAACCAACAAAAAGC
PRIMER_RIGHT_SEQUENCE=TTCCGATAGGAAAACCGAGTTA
PRIMER_LEFT=293,22
PRIMER_RIGHT=573,22
PRIMER_LEFT_TM=59.793
PRIMER_RIGHT_TM=59.964
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.3211
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.283783
PRIMER_LEFT_1_SEQUENCE=CATCTCAAAGCCAACATTGTGT
PRIMER_RIGHT_1_SEQUENCE=CCTCCTTAATTATCTCCGCTCA
PRIMER_LEFT_1=245,22
PRIMER_RIGHT_1=462,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.716
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=218
PRIMER_PAIR_PENALTY_2=0.3435
PRIMER_LEFT_2_PENALTY=0.171757
PRIMER_RIGHT_2_PENALTY=0.171757
PRIMER_LEFT_2_SEQUENCE=GAGTTCCTTTGTACTTGTCGCC
PRIMER_RIGHT_2_SEQUENCE=GGAAAACCGAGTTAGCTGTGTC
PRIMER_LEFT_2=268,22
PRIMER_RIGHT_2=565,22
PRIMER_LEFT_2_TM=60.172
PRIMER_RIGHT_2_TM=60.172
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=0.3601
PRIMER_LEFT_3_PENALTY=0.037339
PRIMER_RIGHT_3_PENALTY=0.322728
PRIMER_LEFT_3_SEQUENCE=CATCTCAAAGCCAACATTGTGT
PRIMER_RIGHT_3_SEQUENCE=ATCCCCTCCTTAATTATCTCCG
PRIMER_LEFT_3=245,22
PRIMER_RIGHT_3=466,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=59.677
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.3746
PRIMER_LEFT_4_PENALTY=0.338329
PRIMER_RIGHT_4_PENALTY=0.036271
PRIMER_LEFT_4_SEQUENCE=GGTTACCCAAACCAACAAAAAG
PRIMER_RIGHT_4_SEQUENCE=TTCCGATAGGAAAACCGAGTTA
PRIMER_LEFT_4=292,22
PRIMER_RIGHT_4=573,22
PRIMER_LEFT_4_TM=59.662
PRIMER_RIGHT_4_TM=59.964
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_05557 Ps_contig_mira-and-tgicl-ass_franssen_38584
SEQUENCE=GAGCAAAACTTGTGGTGTACATCACTGATAAATAGGACCTTTACAAGATAATCTTCACTTTGTTGTTTTATTCTTACACCTATATAAAATTGTTCTATCTTTCAAAAAATTATACAATTTCTGGGGCTTCAGTGGGGAATATGTCCATGGGAAAGTTGACGGTAAACAGTAAATAAATAAACCATGTCTGGAAATCCAATTTATGGTCAAATAATGAAATTCCAGTAACTCAAATATAATCTCAATAAAATGCCCGTGATTCTTATATATATAAAAAAGAACCTGTGATCCTTGTGAATGCAAGTACTTCGGTTGAATTTATGGATCATCTACTTAGTATTGTGAGGTGGGGACACTGAGAATAAAANTTGGTATTCTTGAAATCATCCAAGAGTGNATTGTATCCATCCTTCGTTCTCGTTTTTGTTGGGCAAACCAAACTCTTCCATGRAAGCCGATGTTTCCTAGCGTAATAGTTCTCCTTYTCTTTCCCTTTTTCGATGAATGGTCATCTTGAAGTTTTAGCCACCCAGAACAATAATGATCAAGTCGTTCCAAAAGCCAAAAGTCTGTAGGAAAGAAGATATCAGCATCACCCTTAGCCTCCATATAATCTTGGTAATCTGTGCTGCTCCCANNTCTTTTTTAGTTGAAACCAATGGAGCTCGTTCACCAGGTATCTTCACATCTGGCAAGTAACTGAAATCAGAAGCAATCAAAGACATCTTTGGTAGCACCTCAT
TARGET=170,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05558 Ps_contig_mira-and-tgicl-ass_franssen_38584
SEQUENCE=GAGCAAAACTTGTGGTGTACATCACTGATAAATAGGACCTTTACAAGATAATCTTCACTTTGTTGTTTTATTCTTACACCTATATAAAATTGTTCTATCTTTCAAAAAATTATACAATTTCTGGGGCTTCAGTGGGGAATATGTCCATGGGAAAGTTGACGGTAAACAGTAAATAAATAAACCATGTCTGGAAATCCAATTTATGGTCAAATAATGAAATTCCAGTAACTCAAATATAATCTCAATAAAATGCCCGTGATTCTTATATATATAAAAAAGAACCTGTGATCCTTGTGAATGCAAGTACTTCGGTTGAATTTATGGATCATCTACTTAGTATTGTGAGGTGGGGACACTGAGAATAAAANTTGGTATTCTTGAAATCATCCAAGAGTGNATTGTATCCATCCTTCGTTCTCGTTTTTGTTGGGCAAACCAAACTCTTCCATGRAAGCCGATGTTTCCTAGCGTAATAGTTCTCCTTYTCTTTCCCTTTTTCGATGAATGGTCATCTTGAAGTTTTAGCCACCCAGAACAATAATGATCAAGTCGTTCCAAAAGCCAAAAGTCTGTAGGAAAGAAGATATCAGCATCACCCTTAGCCTCCATATAATCTTGGTAATCTGTGCTGCTCCCANNTCTTTTTTAGTTGAAACCAATGGAGCTCGTTCACCAGGTATCTTCACATCTGGCAAGTAACTGAAATCAGAAGCAATCAAAGACATCTTTGGTAGCACCTCAT
TARGET=264,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05559 Ps_contig_mira-and-tgicl-ass_franssen_38586
SEQUENCE=CCAAGGATCATCACTGGGAGGATTCTCCAATTTGCCACAGAAGCAGAGGTAGGTTTTGGGGATGAGGCTTTTGGGATATTCTACCCTACATTTGGGGCAATTCCAGAGAGATGTTTCGGAGGCTTGATCCTGGGAAATGGAAAGGCGAGTGACGGCACGTGCGGCGGCGAGATCGGAAGCTTGACGGGACCAGCTTTGGATGCAGAAGAGATGGAAGACTGCGAAGCAGAGGGAGGTGCAGGACCAGGTGGGATCGGAAGGTTTGATGCGYTCAAGACATATGAGACAGGAAAGRGCGCCGGAGGAGGAGGAGGTGAGGAAGGATTGGATCTTGGATAGGTCGGCGGAGGTGGCATTGGTTGAGGAGTGGCCGGAGAAATTGAAATAGGATTTGAATATCGATTCGGAGAGGTCTGAGTGGCGGAGTAGTTTGGAGGTGGATGATTCCG
TARGET=301,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05560 Ps_contig_mira-and-tgicl-ass_franssen_38587
SEQUENCE=TGAAGGATACTATTAGACTGTTTAATAAACACTATCTCCATTTGGTTCAGGAGGAAGAGGAGAGGTGTGCTAAAGCAAGAGCTGAAGCTGAGAAGAAGAAGAAATCTCCCAAGAAGTCTCCTAAGAAATCTCCTAAGAAATCTCCTGGGAAGAAATCTCCCAAAAAATCTAAGTCATCTGCTGGGAAGAAAAATGCTAAGAAAGATACTTCGCATATAGAACCAAAGAGCGCAGCCAAACGACCTGATCTGAAGGCCTTAACCAAGATGATGCAAAAATAGCGAGATACTGCACCCTGAAAAAAGAATTGGCGATATTCCAGGTGTTGAGGTTGGATATCAGTTCTATTCTCGTGCCGAAATGGTTGCTATTGGTTTTCATTAGCCACTGGTTAAATGGAATTGATTATATGGGCCAATCTTATTCCAAAGTGTACAACAGCTA
TARGET=91,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1045
PRIMER_LEFT_PENALTY=0.016312
PRIMER_RIGHT_PENALTY=0.088198
PRIMER_LEFT_SEQUENCE=GAGGAAGAGGAGAGGTGTGCTA
PRIMER_RIGHT_SEQUENCE=GGAGATTTCTTCCCAGGAGATT
PRIMER_LEFT=50,22
PRIMER_RIGHT=159,22
PRIMER_LEFT_TM=60.016
PRIMER_RIGHT_TM=59.912
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=0.1524
PRIMER_LEFT_1_PENALTY=0.016312
PRIMER_RIGHT_1_PENALTY=0.136083
PRIMER_LEFT_1_SEQUENCE=GAGGAAGAGGAGAGGTGTGCTA
PRIMER_RIGHT_1_SEQUENCE=TCTTAGCATTTTTCTTCCCAGC
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=200,22
PRIMER_LEFT_1_TM=60.016
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=0.1630
PRIMER_LEFT_2_PENALTY=0.074807
PRIMER_RIGHT_2_PENALTY=0.088198
PRIMER_LEFT_2_SEQUENCE=AGGAGAGGTGTGCTAAAGCAAG
PRIMER_RIGHT_2_SEQUENCE=GGAGATTTCTTCCCAGGAGATT
PRIMER_LEFT_2=57,22
PRIMER_RIGHT_2=159,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=59.912
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.1636
PRIMER_LEFT_3_PENALTY=0.016312
PRIMER_RIGHT_3_PENALTY=0.147295
PRIMER_LEFT_3_SEQUENCE=GAGGAAGAGGAGAGGTGTGCTA
PRIMER_RIGHT_3_SEQUENCE=TGATATCCAACCTCAACACCTG
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=340,22
PRIMER_LEFT_3_TM=60.016
PRIMER_RIGHT_3_TM=59.853
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.2027
PRIMER_LEFT_4_PENALTY=0.114470
PRIMER_RIGHT_4_PENALTY=0.088198
PRIMER_LEFT_4_SEQUENCE=GGAAGAGGAGAGGTGTGCTAAA
PRIMER_RIGHT_4_SEQUENCE=GGAGATTTCTTCCCAGGAGATT
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=159,22
PRIMER_LEFT_4_TM=59.886
PRIMER_RIGHT_4_TM=59.912
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_05561 Ps_contig_mira-and-tgicl-ass_franssen_38591
SEQUENCE=CGGGGATTCATTGCCCACCATCTGTTTGCTATATTGCCCCACTCACAAAACAACAAGAAAGAAAAGGGAATTTCTTCGTTCTTGTTTGTTTGATTGATTGATTGATTTCAGAGAGGAGAAGATAATTAATAATTGAGGTGTGGTGAAGAATTTTGTTGGATTGAAGGTAGGAGGAGGATAAACGACCCCACCTACTTTTCACATTCATCATCACCATCCTCTTCATCAATCTCTTTTTTATCAATCTTCTCTTCTCTACTTTGTTTTTGTAGATCTTGAGGTGTTTTTACTTCACATACCAACTTGCTAGATCTATTTCTATATTTTTTTATTCTATCTTAACATTAATTTATTCCATACAAGATTTGTTCACCAATCCAAGAGATTTTCTTATTCTGCATATATTGTTCTTATTATATATGATTGAATGTTGCTTGGGATGTGTTTGTGAAAAGTATTCAAAGAGAGTTATTAGTAGTATGCTGGTTAAGCTTTGTTTTGTGACCAAACAGACATAGCAGTGTTCATTCTTGTGGCTGTCTACCTGATCTAAAGCTTTGATTTGGGAGGCCCACCATTGGGTTTTGGTGGGCTTTTCATTGAGCCAATAAAAAACCCATCTTTAAAGGTTTCCAAATTGCTTCAGTGTTTCTAAAAAAGGGAGGGAAAGTGTCTGTCAATTGTCATTTGTGTTAGCCATTTATGTTCCATATATTTATATATATATGTAAAGCTCAAACTTGATACTTGAAAGATATTTTTCTATCCAATTTTCTAATGCTTCTTTACTTTATTACTTGTGTCTCTTTCTTTCTGTTTTTGAAACCACTTCTCCTTCTAAAACCACTTCCATCATGGACAAATGAGGTGAGGTTGATCTCGCTTTTGTTTCACAAGGACTTGTGTGTCAAATCCATCTCTAAGTTGTTTAGGAAGACGTTTGTTTCAAGAATGTCTTTTGTGAAGAAGAGACTTGTTTCAAAAGTAGAGAATGTCGAGGAGGAGGAGGAGGAAGTGGAGGAGGAGAAAGACTCGTCACAACAGGATTTGTCTGTCTTGGATTTGCCTGATCTTGTGGTTAGGATAGCTATTCGTAGAAACCG
TARGET=90,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0534
PRIMER_LEFT_PENALTY=0.045112
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=CCCACTCACAAAACAACAAGAA
PRIMER_RIGHT_SEQUENCE=CAAAATTCTTCACCACACCTCA
PRIMER_LEFT=37,22
PRIMER_RIGHT=154,22
PRIMER_LEFT_TM=60.045
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=118
PRIMER_PAIR_PENALTY_1=0.1737
PRIMER_LEFT_1_PENALTY=0.045112
PRIMER_RIGHT_1_PENALTY=0.128624
PRIMER_LEFT_1_SEQUENCE=CCCACTCACAAAACAACAAGAA
PRIMER_RIGHT_1_SEQUENCE=AGTAGGTGGGGTCGTTTATCCT
PRIMER_LEFT_1=37,22
PRIMER_RIGHT_1=195,22
PRIMER_LEFT_1_TM=60.045
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.2426
PRIMER_LEFT_2_PENALTY=0.045112
PRIMER_RIGHT_2_PENALTY=0.197454
PRIMER_LEFT_2_SEQUENCE=CCCACTCACAAAACAACAAGAA
PRIMER_RIGHT_2_SEQUENCE=ATCCTCCTCCTACCTTCAATCC
PRIMER_LEFT_2=37,22
PRIMER_RIGHT_2=178,22
PRIMER_LEFT_2_TM=60.045
PRIMER_RIGHT_2_TM=59.803
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.2476
PRIMER_LEFT_3_PENALTY=0.045112
PRIMER_RIGHT_3_PENALTY=0.202525
PRIMER_LEFT_3_SEQUENCE=CCCACTCACAAAACAACAAGAA
PRIMER_RIGHT_3_SEQUENCE=GGTCGTTTATCCTCCTCCTACC
PRIMER_LEFT_3=37,22
PRIMER_RIGHT_3=186,22
PRIMER_LEFT_3_TM=60.045
PRIMER_RIGHT_3_TM=60.203
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=150
PRIMER_PAIR_PENALTY_4=0.2476
PRIMER_LEFT_4_PENALTY=0.045112
PRIMER_RIGHT_4_PENALTY=0.202525
PRIMER_LEFT_4_SEQUENCE=CCCACTCACAAAACAACAAGAA
PRIMER_RIGHT_4_SEQUENCE=GGGTCGTTTATCCTCCTCCTAC
PRIMER_LEFT_4=37,22
PRIMER_RIGHT_4=187,22
PRIMER_LEFT_4_TM=60.045
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_05562 Ps_contig_mira-and-tgicl-ass_franssen_38591
SEQUENCE=CGGGGATTCATTGCCCACCATCTGTTTGCTATATTGCCCCACTCACAAAACAACAAGAAAGAAAAGGGAATTTCTTCGTTCTTGTTTGTTTGATTGATTGATTGATTTCAGAGAGGAGAAGATAATTAATAATTGAGGTGTGGTGAAGAATTTTGTTGGATTGAAGGTAGGAGGAGGATAAACGACCCCACCTACTTTTCACATTCATCATCACCATCCTCTTCATCAATCTCTTTTTTATCAATCTTCTCTTCTCTACTTTGTTTTTGTAGATCTTGAGGTGTTTTTACTTCACATACCAACTTGCTAGATCTATTTCTATATTTTTTTATTCTATCTTAACATTAATTTATTCCATACAAGATTTGTTCACCAATCCAAGAGATTTTCTTATTCTGCATATATTGTTCTTATTATATATGATTGAATGTTGCTTGGGATGTGTTTGTGAAAAGTATTCAAAGAGAGTTATTAGTAGTATGCTGGTTAAGCTTTGTTTTGTGACCAAACAGACATAGCAGTGTTCATTCTTGTGGCTGTCTACCTGATCTAAAGCTTTGATTTGGGAGGCCCACCATTGGGTTTTGGTGGGCTTTTCATTGAGCCAATAAAAAACCCATCTTTAAAGGTTTCCAAATTGCTTCAGTGTTTCTAAAAAAGGGAGGGAAAGTGTCTGTCAATTGTCATTTGTGTTAGCCATTTATGTTCCATATATTTATATATATATGTAAAGCTCAAACTTGATACTTGAAAGATATTTTTCTATCCAATTTTCTAATGCTTCTTTACTTTATTACTTGTGTCTCTTTCTTTCTGTTTTTGAAACCACTTCTCCTTCTAAAACCACTTCCATCATGGACAAATGAGGTGAGGTTGATCTCGCTTTTGTTTCACAAGGACTTGTGTGTCAAATCCATCTCTAAGTTGTTTAGGAAGACGTTTGTTTCAAGAATGTCTTTTGTGAAGAAGAGACTTGTTTCAAAAGTAGAGAATGTCGAGGAGGAGGAGGAGGAAGTGGAGGAGGAGAAAGACTCGTCACAACAGGATTTGTCTGTCTTGGATTTGCCTGATCTTGTGGTTAGGATAGCTATTCGTAGAAACCG
TARGET=717,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6129
PRIMER_LEFT_PENALTY=0.380820
PRIMER_RIGHT_PENALTY=0.232060
PRIMER_LEFT_SEQUENCE=AAAAGGGAGGGAAAGTGTCTGT
PRIMER_RIGHT_SEQUENCE=GAAACAAAAGCGAGATCAACCT
PRIMER_LEFT=655,22
PRIMER_RIGHT=891,22
PRIMER_LEFT_TM=60.381
PRIMER_RIGHT_TM=59.768
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.6140
PRIMER_LEFT_1_PENALTY=0.380820
PRIMER_RIGHT_1_PENALTY=0.233224
PRIMER_LEFT_1_SEQUENCE=AAAAGGGAGGGAAAGTGTCTGT
PRIMER_RIGHT_1_SEQUENCE=ATCAACCTCACCTCATTTGTCC
PRIMER_LEFT_1=655,22
PRIMER_RIGHT_1=877,22
PRIMER_LEFT_1_TM=60.381
PRIMER_RIGHT_1_TM=60.233
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.6247
PRIMER_LEFT_2_PENALTY=0.392624
PRIMER_RIGHT_2_PENALTY=0.232060
PRIMER_LEFT_2_SEQUENCE=GGTTTCCAAATTGCTTCAGTGT
PRIMER_RIGHT_2_SEQUENCE=GAAACAAAAGCGAGATCAACCT
PRIMER_LEFT_2=627,22
PRIMER_RIGHT_2=891,22
PRIMER_LEFT_2_TM=60.393
PRIMER_RIGHT_2_TM=59.768
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.6258
PRIMER_LEFT_3_PENALTY=0.392624
PRIMER_RIGHT_3_PENALTY=0.233224
PRIMER_LEFT_3_SEQUENCE=GGTTTCCAAATTGCTTCAGTGT
PRIMER_RIGHT_3_SEQUENCE=ATCAACCTCACCTCATTTGTCC
PRIMER_LEFT_3=627,22
PRIMER_RIGHT_3=877,22
PRIMER_LEFT_3_TM=60.393
PRIMER_RIGHT_3_TM=60.233
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=0.7459
PRIMER_LEFT_4_PENALTY=0.513831
PRIMER_RIGHT_4_PENALTY=0.232060
PRIMER_LEFT_4_SEQUENCE=AGGTTTCCAAATTGCTTCAGTG
PRIMER_RIGHT_4_SEQUENCE=GAAACAAAAGCGAGATCAACCT
PRIMER_LEFT_4=626,22
PRIMER_RIGHT_4=891,22
PRIMER_LEFT_4_TM=60.514
PRIMER_RIGHT_4_TM=59.768
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_05563 Ps_contig_mira-and-tgicl-ass_franssen_38591
SEQUENCE=CGGGGATTCATTGCCCACCATCTGTTTGCTATATTGCCCCACTCACAAAACAACAAGAAAGAAAAGGGAATTTCTTCGTTCTTGTTTGTTTGATTGATTGATTGATTTCAGAGAGGAGAAGATAATTAATAATTGAGGTGTGGTGAAGAATTTTGTTGGATTGAAGGTAGGAGGAGGATAAACGACCCCACCTACTTTTCACATTCATCATCACCATCCTCTTCATCAATCTCTTTTTTATCAATCTTCTCTTCTCTACTTTGTTTTTGTAGATCTTGAGGTGTTTTTACTTCACATACCAACTTGCTAGATCTATTTCTATATTTTTTTATTCTATCTTAACATTAATTTATTCCATACAAGATTTGTTCACCAATCCAAGAGATTTTCTTATTCTGCATATATTGTTCTTATTATATATGATTGAATGTTGCTTGGGATGTGTTTGTGAAAAGTATTCAAAGAGAGTTATTAGTAGTATGCTGGTTAAGCTTTGTTTTGTGACCAAACAGACATAGCAGTGTTCATTCTTGTGGCTGTCTACCTGATCTAAAGCTTTGATTTGGGAGGCCCACCATTGGGTTTTGGTGGGCTTTTCATTGAGCCAATAAAAAACCCATCTTTAAAGGTTTCCAAATTGCTTCAGTGTTTCTAAAAAAGGGAGGGAAAGTGTCTGTCAATTGTCATTTGTGTTAGCCATTTATGTTCCATATATTTATATATATATGTAAAGCTCAAACTTGATACTTGAAAGATATTTTTCTATCCAATTTTCTAATGCTTCTTTACTTTATTACTTGTGTCTCTTTCTTTCTGTTTTTGAAACCACTTCTCCTTCTAAAACCACTTCCATCATGGACAAATGAGGTGAGGTTGATCTCGCTTTTGTTTCACAAGGACTTGTGTGTCAAATCCATCTCTAAGTTGTTTAGGAAGACGTTTGTTTCAAGAATGTCTTTTGTGAAGAAGAGACTTGTTTCAAAAGTAGAGAATGTCGAGGAGGAGGAGGAGGAAGTGGAGGAGGAGAAAGACTCGTCACAACAGGATTTGTCTGTCTTGGATTTGCCTGATCTTGTGGTTAGGATAGCTATTCGTAGAAACCG
TARGET=997,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3054
PRIMER_LEFT_PENALTY=0.232060
PRIMER_RIGHT_PENALTY=0.073355
PRIMER_LEFT_SEQUENCE=GAGGTTGATCTCGCTTTTGTTT
PRIMER_RIGHT_SEQUENCE=ACAGACAAATCCTGTTGTGACG
PRIMER_LEFT=869,22
PRIMER_RIGHT=1054,22
PRIMER_LEFT_TM=59.768
PRIMER_RIGHT_TM=60.073
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=186
PRIMER_PAIR_PENALTY_1=0.3054
PRIMER_LEFT_1_PENALTY=0.232060
PRIMER_RIGHT_1_PENALTY=0.073355
PRIMER_LEFT_1_SEQUENCE=AGGTTGATCTCGCTTTTGTTTC
PRIMER_RIGHT_1_SEQUENCE=ACAGACAAATCCTGTTGTGACG
PRIMER_LEFT_1=870,22
PRIMER_RIGHT_1=1054,22
PRIMER_LEFT_1_TM=59.768
PRIMER_RIGHT_1_TM=60.073
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=185
PRIMER_PAIR_PENALTY_2=0.3066
PRIMER_LEFT_2_PENALTY=0.233224
PRIMER_RIGHT_2_PENALTY=0.073355
PRIMER_LEFT_2_SEQUENCE=GGACAAATGAGGTGAGGTTGAT
PRIMER_RIGHT_2_SEQUENCE=ACAGACAAATCCTGTTGTGACG
PRIMER_LEFT_2=856,22
PRIMER_RIGHT_2=1054,22
PRIMER_LEFT_2_TM=60.233
PRIMER_RIGHT_2_TM=60.073
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=0.4484
PRIMER_LEFT_3_PENALTY=0.375090
PRIMER_RIGHT_3_PENALTY=0.073355
PRIMER_LEFT_3_SEQUENCE=CATCATGGACAAATGAGGTGAG
PRIMER_RIGHT_3_SEQUENCE=ACAGACAAATCCTGTTGTGACG
PRIMER_LEFT_3=850,22
PRIMER_RIGHT_3=1054,22
PRIMER_LEFT_3_TM=60.375
PRIMER_RIGHT_3_TM=60.073
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=205
PRIMER_PAIR_PENALTY_4=0.5696
PRIMER_LEFT_4_PENALTY=0.496229
PRIMER_RIGHT_4_PENALTY=0.073355
PRIMER_LEFT_4_SEQUENCE=AAATGAGGTGAGGTTGATCTCG
PRIMER_RIGHT_4_SEQUENCE=ACAGACAAATCCTGTTGTGACG
PRIMER_LEFT_4=860,22
PRIMER_RIGHT_4=1054,22
PRIMER_LEFT_4_TM=60.496
PRIMER_RIGHT_4_TM=60.073
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=195
=
PRIMER_SEQUENCE_ID=SSR_05564 Ps_contig_mira-and-tgicl-ass_franssen_38595
SEQUENCE=GTTAAAAGTAAACCGTTTACCTAACCGGGGTTTTTTACCGGTAACGGTTTTTTTACCTTTAACGGTTTTAACCGGGTTAAACGGGGGTTTTTACCGGTTAACGTTTAGGTTTTACGGTTTTTGGGNTAANATTTNGRTAGAGCTTTGTTGTATCAAGTACTCGCCCGGTTAAACGGACATCGACAGGACGAGTGTCGCGGGCGTCCACCTTTACGCGCCATAGCCACAGTCGGTGGTGGAGGAAGTAACCCTGCTCGAAACAGAAGACGCTCAACGGGATTCGAAGGTTGAGCTCCTACAGAAAGCCAATACTTGACCCTGTCAAAGTTGATACCCATTCTTTTGCCACCATCTTGAACAGGTAAGGGATTGTAGTAACCTAGAACTTCGAGGTGTTTGCCATCTCTTGGAGATCTGCTATCGGCAGCCATCACACGGTAGAATGGCTTGTTTTTGCATCCTAGACGCGATAGCCGAATCCTAACCACCATTTCCCCCAAATTCAGCCTCCTGACTCTCCTACTCACTATCAATGTCTTTCAAATCCAGTTGACAGAAACCAAACGAATCTCTCTCTGTGTGGCACTGTGACGGCGTGGCCGGGAGCGACGGAGTTGACGTGACGGCGGCGGAGAATCGCAGGAGAGAGACCGGAAGGGTTTATCGCAAGAGAGAGAGGAGAAGGGCCTAGTGGGACTGAAGTTTGAGAAATTTTTGCTAGNGTCGGAACACGACACGTGGAATTTAGTACAACTGAAAGGTAAANCATTAAAAAAATGATTTTTCTTAAATAAAAAAATTAGAGAATTAATATTAACAGAGAACAAATAACTTCTTTTCTCCGTCTCGGTGAAG
TARGET=669,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05565 Ps_contig_mira-and-tgicl-ass_franssen_38596
SEQUENCE=CAGTTTCTTGCTTCTTTTTCTTGTTTCGATTTCTTGTTGTTGTTGTTGTTGTTGTGATTTATTGTGAATATGGTTTTGGTATTGAATTCTTGAAGGTGATGATGATAATGGAGGGAGTTGTAGAAGTTGATGA
TARGET=34,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05566 Ps_contig_mira-and-tgicl-ass_franssen_38601
SEQUENCE=AAGCAGTGGTATCAAACGCAAGAGTACGTCGGGGAAGACAAACACTTTACCACTTCTAACTATTACTATCTCTTGGTACCTCCGCCATAACTAAAACAAAACCATACTACACCTTACTTGAATGCACCCTTTCTTCCACCATTCCCATTCTCGCATTCTTCGCAATCCTTTTTCTCTCTCTACAATGTCTTCTTCTCTCACTCTTCCCTCCTTTCCCCTATCTTCCCCACTCTCTCACCTCAAACCCTCTCTCTTCAAAACCATCACTTCCCTCGATCTAGGGTTACCGCGAGCATCTCCACCGGATCTCAGGCTTCCATCCATGACGCTTCCTTAGCGGATTATAAAGTCTCCAATGCCTTCCTCTTCCCCGGCCAGGGTGCTCAAGCTATTGGAATGGGAAAAGAAGCTCAGAATGTTCCTGCTGCTGCAATTTTGTACAAGAAGGCTAATGAGATATTAGGGTTTGATCTTCTTGATGTATGCGTTAATGGGCCGAAGGAAAAGCTGAATTCGACCGTTATCAGTCAGCCTGCCCTTTACGTCACCAGTCTTGCTGCTGTTGAGCTACTTCGTGCGCGTGAGGGAGGTGAGCAGATTATTGATTCCGTTGATGTTACATGTGGTTTGAGCCTAGGAGAATATACTGCTCTAGCATTTGCTGGGGCTTTCAGCTTTGAAGATGGACTGAAATTGGTGAAACTGAGGGGTGAAGCCATGCAGGATGCTTCTGATGCTGTTAGAAGTGCCATGGTCAGTGTGGTAGGATTGGACTCAGAGAAGGTCCAGCAGTTGTGTGATGCAGCCAATCAGGAAGTGCCTGAAGCTGAGAAGGTTCAAATTGCAAATTTCCTATGTCCAGGAAACTATGCTGTCTCTGGAGGAATAAAAGGAATTGAGGTGGTGGAAGCCAAAGCAAAATCTTTCAAGGCTCGAATGACTGTGCGCCTAGCTGTTGCAGGTGCTTTCCATACTAGTTTTATGGAACCCGCTGTTTCAAGATTGGAAGCAGCATTGGCATCAACAGACATTAGAACCCCAAGAATACCAGTCATTTCCAATGTGAATGCTCAGCCCCATGCAGATCCTGACACAATAAAGAAGATATTGGCCCGACAAGTTACTTCCCCTGTTCAATGGGAAACAACAGTGAAGACTCTTCTATCCAAGGGGCTGAAGAAAGGCTATGAGCTAGGACCCGGAAAGGTTATTGCTGGAATTGTCAAAAGAATGGACAAAAGTACCGAAATTGAAAACATAGGTGCTTAATAAGACTGCAGGACCTTGCTACTTATGTAGCTTCCAATACAAACAAACAACTATAATATTGCATTTTTTAATTTTCATGAGAGAATGAGCGTGAGCTACTCCAAATCACCAGACAGAACGCATGGATGGATCCTGAAGCTTCTGTGAGCAAAGAGCAGCTTCAGTCAGATTTGAATTCCAGTTTTGAAGTTAAAAATATTTAATCCACCAATAGATGCAGTTATGAACAAATTAGATCAGTGTTTGATGTCATTGCTTAGAATTCCAGTTTATTTATGTAATCTTATAGAAAGCTGTACACCTTATCTATATTTCTGAGTTAATATCTACTTAAATCTTTGTATCACTAAATTTTAGATGAAACAATTGTTGATTTAAGATTTGAAATGACATTTGAGGAATAATTAGTTTATATACAATTTAGTCAAATTTATGC
TARGET=1308,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1329
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.124655
PRIMER_LEFT_SEQUENCE=CAATGGGAAACAACAGTGAAGA
PRIMER_RIGHT_SEQUENCE=TCTGGTGATTTGGAGTAGCTCA
PRIMER_LEFT=1134,22
PRIMER_RIGHT=1381,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.875
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.1329
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=TCAATGGGAAACAACAGTGAAG
PRIMER_RIGHT_1_SEQUENCE=TCTGGTGATTTGGAGTAGCTCA
PRIMER_LEFT_1=1133,22
PRIMER_RIGHT_1=1381,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=0.1578
PRIMER_LEFT_2_PENALTY=0.033137
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=CGACAAGTTACTTCCCCTGTTC
PRIMER_RIGHT_2_SEQUENCE=TCTGGTGATTTGGAGTAGCTCA
PRIMER_LEFT_2=1113,22
PRIMER_RIGHT_2=1381,22
PRIMER_LEFT_2_TM=60.033
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=0.2220
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.213725
PRIMER_LEFT_3_SEQUENCE=TCAATGGGAAACAACAGTGAAG
PRIMER_RIGHT_3_SEQUENCE=GTAGCTCACGCTCATTCTCTCA
PRIMER_LEFT_3=1133,22
PRIMER_RIGHT_3=1367,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.786
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.2469
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.213725
PRIMER_LEFT_4_SEQUENCE=CGACAAGTTACTTCCCCTGTTC
PRIMER_RIGHT_4_SEQUENCE=GTAGCTCACGCTCATTCTCTCA
PRIMER_LEFT_4=1113,22
PRIMER_RIGHT_4=1367,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=59.786
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_05567 Ps_contig_mira-and-tgicl-ass_franssen_38606
SEQUENCE=ACCCAAAAAACAAGAAAAAAGACGATCGCTGTTATTCGATTGTATCGTGAAAAATCATCATCTCGTATCAACAACTCTCGATCTCGACCGATCCCAAACCCATGGCGCCGCCGCAGTCGTCGTCGTCGCCGTTCGATCCATTCAACCAAGAACCTGTTGCTAGAAAGAAGAACAATGGAGCCCCAATTAAGTTCCTTTTGCCTCTCATATATGCACCTGTTCTACCTCTAATTCGTTTGTCGCTGCGGAGTAAACCTGTTCTAAGAGATCGTTTGTTTACAGCTGTGTTGGTTGGGGCTTTGGCCCATGGAGGCTATTTGATAACGGATTTGTATGATGCTGAGAGTAAATGATTATGCAGAGCATGTATCGGGCGATAGATCAGTAATGTTTACATCACAACCTCAAGTCCTCAATAAATTATTTGACTTCTGTTTACTCCTCAATAAATTATTTGACTTCTGTTTACTCCTCAATATCCTGGTAAACACCTTATATAGATCAAATACTGTCATGTATGGATATTTTTTCTCCAATTCCTTTTAGAGATTATTTGTGCCATGTACTATTTGAATTTGAGAATTGCATCTTGTATTGCGGAGTTAGCTTCTAGTTGAATAATTCTGGAATTTGGAGTCTGTTATTGATCTCCTCCAAGTAAGTTAAAATGTGAGCTAAGTAATCCTTATTTTTGCAAAG
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1560
PRIMER_LEFT_PENALTY=0.116088
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=CGATCGCTGTTATTCGATTGTA
PRIMER_RIGHT_SEQUENCE=AGCAACAGGTTCTTGGTTGAAT
PRIMER_LEFT=22,22
PRIMER_RIGHT=160,22
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=139
PRIMER_PAIR_PENALTY_1=0.1625
PRIMER_LEFT_1_PENALTY=0.122657
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=AAGACGATCGCTGTTATTCGAT
PRIMER_RIGHT_1_SEQUENCE=AGCAACAGGTTCTTGGTTGAAT
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=160,22
PRIMER_LEFT_1_TM=60.123
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.3000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=0.2330
PRIMER_LEFT_2_PENALTY=0.116088
PRIMER_RIGHT_2_PENALTY=0.116923
PRIMER_LEFT_2_SEQUENCE=CGATCGCTGTTATTCGATTGTA
PRIMER_RIGHT_2_SEQUENCE=CAGGTGCATATATGAGAGGCAA
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=218,22
PRIMER_LEFT_2_TM=60.116
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=197
PRIMER_PAIR_PENALTY_3=0.2396
PRIMER_LEFT_3_PENALTY=0.122657
PRIMER_RIGHT_3_PENALTY=0.116923
PRIMER_LEFT_3_SEQUENCE=AAGACGATCGCTGTTATTCGAT
PRIMER_RIGHT_3_SEQUENCE=CAGGTGCATATATGAGAGGCAA
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=218,22
PRIMER_LEFT_3_TM=60.123
PRIMER_RIGHT_3_TM=60.117
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.3000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=0.2620
PRIMER_LEFT_4_PENALTY=0.116088
PRIMER_RIGHT_4_PENALTY=0.145891
PRIMER_LEFT_4_SEQUENCE=CGATCGCTGTTATTCGATTGTA
PRIMER_RIGHT_4_SEQUENCE=AAAAGGAACTTAATTGGGGCTC
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=198,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=59.854
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=177
=
PRIMER_SEQUENCE_ID=SSR_05568 Ps_contig_mira-and-tgicl-ass_franssen_38607
SEQUENCE=CGGTGCCAAAGTCCAATATCACTCCTATCAATACCTCTCTCTCTCTTAAACCTAAACATTCACACAATTTTGATTTTTCTATTCTCTAGAGTCATCAAATTTTCACTTTATTATTACTACAATGAGCAACATTAAGAACAAAACAGTGAGATTGTTCAAGCAAATTATACCTAGTTTAACCTCAAAAACTATAGCTTTAAAATCCAAAACAAATGCCATCAAAGCTCGTTTAATTTTATTCTCTTTCATGAAGAATAAGAAGCTCCTTATGAGTTCAATTTCAGAGAAGTTTCACTCTCTTTGGGGGAACCATCCTCATCATCACAAGGAAGATTGCTTAATCGAAGGTGGGAATTGTGATTATCATAATAGAGCTATAGTTGTTTATAACAAAAATGCTCACACTTATGAAGCGTTGCTAAATCCAAGTGAATTAGCACAACAAGTTTTGGATGAACAAGATCAAGAAGATGGATATGATGGTTACTATGATAATGATGATGATGATAAGTATCCTGATTTGACTCACAATCTTTTTGATTCTGAGGGTTTGGATTTCGGAGGATCAGTGATTGATAGGGTGAAGAATTGTAAGGAAGAAGCAGGGAAAGAGTTTAAACTTGAGGATGATATTGATGAAGTTGCTGATTCTTTTTATCAGAAGATTTAGAAGAAATATAATCTTGCAGAAACAAGATTCATTGAAGAGAAAGAGACAAATTGCGCAAAATGGTGCCTGAATTTCGAGTTCACTCGGATTCAACATCTCTCCCATTGATGGACAATCTACAAATAATTCAAACAAGTATTCCTAAAAGGTGCACCCTTGAAGGGTTGTTTGATGGTTGTTCCATGAGTTCTTGTCATAAAAGTTGGAATTATTATTGGTGTTTTATTTATGGATGTGAAACTATTGTTAG
TARGET=35,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5357
PRIMER_LEFT_PENALTY=0.249074
PRIMER_RIGHT_PENALTY=2.286660
PRIMER_LEFT_SEQUENCE=GGTGCCAAAGTCCAATATCACT
PRIMER_RIGHT_SEQUENCE=AACGAGCTTTGATGGCATTT
PRIMER_LEFT=1,22
PRIMER_RIGHT=229,20
PRIMER_LEFT_TM=60.249
PRIMER_RIGHT_TM=59.713
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=2.5357
PRIMER_LEFT_1_PENALTY=0.249074
PRIMER_RIGHT_1_PENALTY=2.286660
PRIMER_LEFT_1_SEQUENCE=GGTGCCAAAGTCCAATATCACT
PRIMER_RIGHT_1_SEQUENCE=AAACGAGCTTTGATGGCATT
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=230,20
PRIMER_LEFT_1_TM=60.249
PRIMER_RIGHT_1_TM=59.713
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=2.7998
PRIMER_LEFT_2_PENALTY=0.513182
PRIMER_RIGHT_2_PENALTY=2.286660
PRIMER_LEFT_2_SEQUENCE=GCCAAAGTCCAATATCACTCCT
PRIMER_RIGHT_2_SEQUENCE=AACGAGCTTTGATGGCATTT
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=229,20
PRIMER_LEFT_2_TM=59.487
PRIMER_RIGHT_2_TM=59.713
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=226
PRIMER_PAIR_PENALTY_3=2.7998
PRIMER_LEFT_3_PENALTY=0.513182
PRIMER_RIGHT_3_PENALTY=2.286660
PRIMER_LEFT_3_SEQUENCE=GCCAAAGTCCAATATCACTCCT
PRIMER_RIGHT_3_SEQUENCE=AAACGAGCTTTGATGGCATT
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=230,20
PRIMER_LEFT_3_TM=59.487
PRIMER_RIGHT_3_TM=59.713
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=2.8056
PRIMER_LEFT_4_PENALTY=0.249074
PRIMER_RIGHT_4_PENALTY=2.556510
PRIMER_LEFT_4_SEQUENCE=GGTGCCAAAGTCCAATATCACT
PRIMER_RIGHT_4_SEQUENCE=TTAAACGAGCTTTGATGGCA
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=232,20
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=59.443
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_05569 Ps_contig_mira-and-tgicl-ass_franssen_38607
SEQUENCE=CGGTGCCAAAGTCCAATATCACTCCTATCAATACCTCTCTCTCTCTTAAACCTAAACATTCACACAATTTTGATTTTTCTATTCTCTAGAGTCATCAAATTTTCACTTTATTATTACTACAATGAGCAACATTAAGAACAAAACAGTGAGATTGTTCAAGCAAATTATACCTAGTTTAACCTCAAAAACTATAGCTTTAAAATCCAAAACAAATGCCATCAAAGCTCGTTTAATTTTATTCTCTTTCATGAAGAATAAGAAGCTCCTTATGAGTTCAATTTCAGAGAAGTTTCACTCTCTTTGGGGGAACCATCCTCATCATCACAAGGAAGATTGCTTAATCGAAGGTGGGAATTGTGATTATCATAATAGAGCTATAGTTGTTTATAACAAAAATGCTCACACTTATGAAGCGTTGCTAAATCCAAGTGAATTAGCACAACAAGTTTTGGATGAACAAGATCAAGAAGATGGATATGATGGTTACTATGATAATGATGATGATGATAAGTATCCTGATTTGACTCACAATCTTTTTGATTCTGAGGGTTTGGATTTCGGAGGATCAGTGATTGATAGGGTGAAGAATTGTAAGGAAGAAGCAGGGAAAGAGTTTAAACTTGAGGATGATATTGATGAAGTTGCTGATTCTTTTTATCAGAAGATTTAGAAGAAATATAATCTTGCAGAAACAAGATTCATTGAAGAGAAAGAGACAAATTGCGCAAAATGGTGCCTGAATTTCGAGTTCACTCGGATTCAACATCTCTCCCATTGATGGACAATCTACAAATAATTCAAACAAGTATTCCTAAAAGGTGCACCCTTGAAGGGTTGTTTGATGGTTGTTCCATGAGTTCTTGTCATAAAAGTTGGAATTATTATTGGTGTTTTATTTATGGATGTGAAACTATTGTTAG
TARGET=495,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1071
PRIMER_LEFT_PENALTY=0.080962
PRIMER_RIGHT_PENALTY=0.026163
PRIMER_LEFT_SEQUENCE=TCATCATCACAAGGAAGATTGC
PRIMER_RIGHT_SEQUENCE=CCAAACCCTCAGAATCAAAAAG
PRIMER_LEFT=315,22
PRIMER_RIGHT=553,22
PRIMER_LEFT_TM=60.081
PRIMER_RIGHT_TM=59.974
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.1802
PRIMER_LEFT_1_PENALTY=0.075027
PRIMER_RIGHT_1_PENALTY=0.105201
PRIMER_LEFT_1_SEQUENCE=CGAAGGTGGGAATTGTGATTAT
PRIMER_RIGHT_1_SEQUENCE=ACTCTTTCCCTGCTTCTTCCTT
PRIMER_LEFT_1=342,22
PRIMER_RIGHT_1=613,22
PRIMER_LEFT_1_TM=60.075
PRIMER_RIGHT_1_TM=59.895
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.1802
PRIMER_LEFT_2_PENALTY=0.075027
PRIMER_RIGHT_2_PENALTY=0.105201
PRIMER_LEFT_2_SEQUENCE=CGAAGGTGGGAATTGTGATTAT
PRIMER_RIGHT_2_SEQUENCE=AACTCTTTCCCTGCTTCTTCCT
PRIMER_LEFT_2=342,22
PRIMER_RIGHT_2=614,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=59.895
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.1888
PRIMER_LEFT_3_PENALTY=0.075027
PRIMER_RIGHT_3_PENALTY=0.113803
PRIMER_LEFT_3_SEQUENCE=CGAAGGTGGGAATTGTGATTAT
PRIMER_RIGHT_3_SEQUENCE=CTTTCCCTGCTTCTTCCTTACA
PRIMER_LEFT_3=342,22
PRIMER_RIGHT_3=610,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=0.2923
PRIMER_LEFT_4_PENALTY=0.080962
PRIMER_RIGHT_4_PENALTY=0.211336
PRIMER_LEFT_4_SEQUENCE=TCATCATCACAAGGAAGATTGC
PRIMER_RIGHT_4_SEQUENCE=CCCTATCAATCACTGATCCTCC
PRIMER_LEFT_4=315,22
PRIMER_RIGHT_4=580,22
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=59.789
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_05570 Ps_contig_mira-and-tgicl-ass_franssen_38614
SEQUENCE=ACCTTTTTTTTTCCTTTAAATTACTAAAAAAATAAACAATATAAATAATAAAGTTAAACAAAAAAGAAAACAAAGCTAAAGAACTTGTTTGCAGTAAAGAGCAGTTTCAAATGCAATGCTCAATCCTTTAATCCTATTTGCAGTACAACTTACAGAGGAAACAATGGAGATGAGTGAGAGATTTAGTCCTATTTTCCAAGCACTTGCATTTGGAACTGATAACAAAGCTACAAATGTTGATATTATGGTTGCACTAATGGAGTATAACAAGACTAAACTTAGTTCATATGGGAACTCCTTCATATGGACCTGTAAAATGTACCATAGAGAAACCAGAAAATAGTCCACTATTAGCATAATGCAAGCAATGGCCCAACTTGAATCCCCTGACTTGAGAAATTCATTTGATTGATGAAGTGATGAGGAATTCAAGATGGATTTTCCTTTGTAGAAAGTCAAGACAAAAGCACCTGCTATTGATATTATGCTTCCCATTACCTTAGCATTGCTACTTCTACTCTTTATAGCTAACTTTTCCATCTTTAACAAGTGTTGGAGAACTATAATTAATACTAATATACCCCAAAATCTGAGATCCACATCTGCATAACAGCACCATACAATTCCAAAAGAACACAAAATCATCAGATTCAAAAGGCCTTAATATTATACAATCACAGTTGTTAAGTTAAGTATAAGGCAATTTTGTCATTATCCTATTGCCCCAAGAAGCAAACTTTTGCACATTATGGAGAAATTCAGTGGAACAGGAACCACTCTTGATCATATACTATGGAGTGACACCAATTGGTACTTGAGAATCAGTTTCGATGAATCCCTAACTAAAAGTCAACAAAACAAAACTCTATTTTCCAGAGACCTCCACTAAAGTACCGATTAGAAACTCATTATAGGCATAGCATGATCGTATCTCATTCTTAATATGTTCAACATATTTCTTCCTTGTCGCCTCATCTTGCCTCAATGACTTAAAATTGGAAAGAACGCGGACAATCTCTAATCCTCCTTTTAAGATTGGAAAGATCGGGGGACGCAAGATGGAGTGATCATATCTCTGTTTCTCTTGCTGCTACAAGTACTTCGACTACTCGTTGAGACACAACAGACCATTTTCTTCAATTCCAGAATGCCTATGAAATTCATGTTCCAGTATTATTGTTGATCAAATCTTTTAGAGTTGAAATTGTGATTGAATATCTCAGTTTTTCATAAACCATTTCACTGAACATCTCTATTTGCTTCTCGTATGTGTTTTACATGAAACAATACCTTTCATGTACTACGATTCAAAAATATTATAGCTTCATCCTCATGTCATTCCTTGCCTACGATTTAGTCCTTGCTCCATCTTTAACCATAGCTCGTACATTTGCTTGTAACATGAAGATGGATCTGTCGGTAACCTCTTTAGGACTATTGACTGGTTGTGAGTAATAAAACAATTTGAATATGGTGGTGGTGGGTAGTTTTGAAGGTTGGTGGTGGTGTGGGTTGAGGGGATGGATGAAGGTGTGTATCCGGCGGCGGCGGCGACTTGTTAGGGATTTCGATGGTAATGAAAATGAAAGAAGGGAAATGCACAGCCNCYNNNTCCGCCG
TARGET=1472,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05571 Ps_contig_mira-and-tgicl-ass_franssen_38614
SEQUENCE=ACCTTTTTTTTTCCTTTAAATTACTAAAAAAATAAACAATATAAATAATAAAGTTAAACAAAAAAGAAAACAAAGCTAAAGAACTTGTTTGCAGTAAAGAGCAGTTTCAAATGCAATGCTCAATCCTTTAATCCTATTTGCAGTACAACTTACAGAGGAAACAATGGAGATGAGTGAGAGATTTAGTCCTATTTTCCAAGCACTTGCATTTGGAACTGATAACAAAGCTACAAATGTTGATATTATGGTTGCACTAATGGAGTATAACAAGACTAAACTTAGTTCATATGGGAACTCCTTCATATGGACCTGTAAAATGTACCATAGAGAAACCAGAAAATAGTCCACTATTAGCATAATGCAAGCAATGGCCCAACTTGAATCCCCTGACTTGAGAAATTCATTTGATTGATGAAGTGATGAGGAATTCAAGATGGATTTTCCTTTGTAGAAAGTCAAGACAAAAGCACCTGCTATTGATATTATGCTTCCCATTACCTTAGCATTGCTACTTCTACTCTTTATAGCTAACTTTTCCATCTTTAACAAGTGTTGGAGAACTATAATTAATACTAATATACCCCAAAATCTGAGATCCACATCTGCATAACAGCACCATACAATTCCAAAAGAACACAAAATCATCAGATTCAAAAGGCCTTAATATTATACAATCACAGTTGTTAAGTTAAGTATAAGGCAATTTTGTCATTATCCTATTGCCCCAAGAAGCAAACTTTTGCACATTATGGAGAAATTCAGTGGAACAGGAACCACTCTTGATCATATACTATGGAGTGACACCAATTGGTACTTGAGAATCAGTTTCGATGAATCCCTAACTAAAAGTCAACAAAACAAAACTCTATTTTCCAGAGACCTCCACTAAAGTACCGATTAGAAACTCATTATAGGCATAGCATGATCGTATCTCATTCTTAATATGTTCAACATATTTCTTCCTTGTCGCCTCATCTTGCCTCAATGACTTAAAATTGGAAAGAACGCGGACAATCTCTAATCCTCCTTTTAAGATTGGAAAGATCGGGGGACGCAAGATGGAGTGATCATATCTCTGTTTCTCTTGCTGCTACAAGTACTTCGACTACTCGTTGAGACACAACAGACCATTTTCTTCAATTCCAGAATGCCTATGAAATTCATGTTCCAGTATTATTGTTGATCAAATCTTTTAGAGTTGAAATTGTGATTGAATATCTCAGTTTTTCATAAACCATTTCACTGAACATCTCTATTTGCTTCTCGTATGTGTTTTACATGAAACAATACCTTTCATGTACTACGATTCAAAAATATTATAGCTTCATCCTCATGTCATTCCTTGCCTACGATTTAGTCCTTGCTCCATCTTTAACCATAGCTCGTACATTTGCTTGTAACATGAAGATGGATCTGTCGGTAACCTCTTTAGGACTATTGACTGGTTGTGAGTAATAAAACAATTTGAATATGGTGGTGGTGGGTAGTTTTGAAGGTTGGTGGTGGTGTGGGTTGAGGGGATGGATGAAGGTGTGTATCCGGCGGCGGCGGCGACTTGTTAGGGATTTCGATGGTAATGAAAATGAAAGAAGGGAAATGCACAGCCNCYNNNTCCGCCG
TARGET=1540,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05572 Ps_contig_mira-and-tgicl-ass_franssen_38620
SEQUENCE=GTAAANNAAATCTTGTAGNTAAAAAGTTGCAAGCAGCAAACAGGGGGAAAATCCAATTTGTTAACAAATGACTGTGTTAACAATTTATTTTACAGNTTAATATAGTGNTTATAATATAAGCACTTATTAATAAGTCATTTGTACAAGAAYACAGAATAAAGTGAAGCAAGATAAATGTGAATCCAATCAGATCCGTAGTGGTAACAGAGAAGAGAAAGCATATGTAAGAAAATGTTAGGGTTTGAAGAAATGAAAAGAAAACCTACAGTACAATAGAACAGAATTAAGAGGAGGATTGAGTAGTAGTAGTAGTATCGTTATTATTGGGGAACGTTTGCTTGAATGAATCCTGGCGCTGTAACCACATCATGTGTCCCTGGAGAGCTGCAGCCCAGGTGATAAGGAAAGATGCAARCCAGAATTTCTTGTCCTTCATCACTTTTTTCAATTCCATTTTCTCATTCACTCAGACAACCAACCTAGAACAAAACAAAATTAACAAACCCCCG
TARGET=299,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05573 Ps_contig_mira-and-tgicl-ass_franssen_38631
SEQUENCE=CCCTTCTGTCTTATCTGTTTTTTTTTGGTGTGCAAATATCTTTACTTGTAAACNAAAAATACTTCATTTTTGCACATGTTTGTGTGTGTGAAATGGTTGAAGAATGTTTTATATTAGTTCTCAAAGTTTATTTATACCTGCTGAAGATATTTTTATCACTAGGCTTTCTTTTTCAATTTGGATCATCGATTGAGAATTGAGATTGACGCAAACGGATATTTAATGAGCAATCCAAGAAAGGGTTAGATTTACTTTTAGTTTCTAGACCTATATATGGTGTGGACTATGCTAGCTTGAAGGTATATAATTTTGAAGTCCGGTAAGGTATAAGTGTGTGCATGTATGTTTGTAGGTGTAACTAAAAAGTAAGATTGCAACATATAATAGGATTCCAAGTTAGTTGTTTTACGGAAAATTTAGTATATGTGCACAATGCAAATTAAGGTGCTACGACAACCTCTGCTTTAATATACAATGCTTACTATCCTGTTTATACGTTTTGCTTCTCACATGTTCCTTTACACACTCACTCATTCAAGAAAAACCACTTTCTGAACCAATAATAAATATTATTTAAAAATTCTATTTTGTATCTTGTGTTGTAACATGTGTCAAATACTGATTCATGCTACCCCATGTGATTAGTATCTCATTTGGGCTTCTCTCTAACTCTATTAGGTGTTTGATTTATTTGACACAAAGCACAATGGGATCCTGGGTTTTGAAGAGTTTGCTCGCGCCTTATCCGTTTTCCATCCAAATGCCCCTATTGATGATAAGATTGAATGTATGTTGTATATTTTTCTTTTAGCTTCTAAATTTTAAGAAGAAAGTACATTTGGAGTCAATTCTTTAATGAGAAAAGAGGTGATGCACCATTAGTGTAAACTAGTTTTACACGGATGTCCAATGAGAATCCGATGTTATGCGGTTATCAGTAACAAAATGAGTTTACGTGGCAATATCATGAGTTTTCATTTTATGTCAATGTAAAACTATTTTACACCAACAATGCATCACACTGAAATTCTTTAATTTATACACAACATGCATCAGAGTGGAACTCTTCTTTAATTTACACCATCATTCTCTGTAAATAAATGATAACTAAACCACTGTAATATCATATTCCAGTTTCCTTTCAATTATACGATCTCAAGCAGCAGGGTT
TARGET=81,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05574 Ps_contig_mira-and-tgicl-ass_franssen_38641
SEQUENCE=GTTGTCTGGCTCTTATCCAAGCTGCAACTGATGGTCAAGAACAAGGGGGAGGTACATGACAGTGATCATTTTTTGCATGCTATTAGAGACCTACTGAAAATTTATTCAAACACTCAAGCTGCACTGTCGACATACGTATCAGCACCAGGCATTGTCCAACAAATATCAGCCCTTCATTCAGATTTAATGACACTTCAATCGGACCTTGATAATTCTCTTCCAGAAGAACGAAATAGGTGTATTAATGAACTGTGCAACCTTATTCAAAGTATGCAACAACTGCTCTTTGCATCTTCAACAACTGCACAGCCAATACTGACCCCTCGGCCATTGATGAAAGAGCTCGATGAGATGGAAAAGATTAACGCAAAGCTCTCTGTTGCTGTGGAAGAGGTGACACTAGAGCATGTCAAGAAAAATGAGATTGTAAAACACCATTCGCAAGAAATAGGACTTCAACGGCGCGTCTTTGTAGACTTCTTTTGCAATCCAGAAAGGCTCAGAAGCCAAGTTAGAGAACTGACTGCCAGGGTCAGAGCCTTGCAAATTTCCTAGTAATCATTAAATAGTTTCTCTATTTTCCACCCTCACTTACCTTCTTTAAGGTTAGTTTTTTTCTTCTTTTCCCTCTACATTATCTCCATCTCCAAGTGTGGAATGTGCTATTCTACACGTGTTTTAAGATTCAGCTATGTATTCACTGAATTGTGTATATTGTAGAAAAGTTATACAACAACAACAACCTTCTTCACATTTTTCGCGATTGATGCCAGTAAGCAACTCAACTTAGAACTCTTTCGTAAGATTGAAAAAGAACAAGGAACTCTTCCTTGTGGATTAACTGATGTGGGATATTTAACT
TARGET=730,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2466
PRIMER_LEFT_PENALTY=0.021195
PRIMER_RIGHT_PENALTY=0.225403
PRIMER_LEFT_SEQUENCE=GAAAGGCTCAGAAGCCAAGTTA
PRIMER_RIGHT_SEQUENCE=TGAGTTGCTTACTGGCATCAAT
PRIMER_LEFT=492,22
PRIMER_RIGHT=783,22
PRIMER_LEFT_TM=60.021
PRIMER_RIGHT_TM=59.775
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=0.3765
PRIMER_LEFT_1_PENALTY=0.151106
PRIMER_RIGHT_1_PENALTY=0.225403
PRIMER_LEFT_1_SEQUENCE=GGCTCAGAAGCCAAGTTAGAGA
PRIMER_RIGHT_1_SEQUENCE=TGAGTTGCTTACTGGCATCAAT
PRIMER_LEFT_1=496,22
PRIMER_RIGHT_1=783,22
PRIMER_LEFT_1_TM=60.151
PRIMER_RIGHT_1_TM=59.775
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=0.5511
PRIMER_LEFT_2_PENALTY=0.325733
PRIMER_RIGHT_2_PENALTY=0.225403
PRIMER_LEFT_2_SEQUENCE=AAGGCTCAGAAGCCAAGTTAGA
PRIMER_RIGHT_2_SEQUENCE=TGAGTTGCTTACTGGCATCAAT
PRIMER_LEFT_2=494,22
PRIMER_RIGHT_2=783,22
PRIMER_LEFT_2_TM=59.674
PRIMER_RIGHT_2_TM=59.775
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.7260
PRIMER_LEFT_3_PENALTY=0.500551
PRIMER_RIGHT_3_PENALTY=0.225403
PRIMER_LEFT_3_SEQUENCE=TTTTCCACCCTCACTTACCTTC
PRIMER_RIGHT_3_SEQUENCE=TGAGTTGCTTACTGGCATCAAT
PRIMER_LEFT_3=577,22
PRIMER_RIGHT_3=783,22
PRIMER_LEFT_3_TM=59.499
PRIMER_RIGHT_3_TM=59.775
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=0.7266
PRIMER_LEFT_4_PENALTY=0.501224
PRIMER_RIGHT_4_PENALTY=0.225403
PRIMER_LEFT_4_SEQUENCE=TCCAAGTGTGGAATGTGCTATT
PRIMER_RIGHT_4_SEQUENCE=TGAGTTGCTTACTGGCATCAAT
PRIMER_LEFT_4=645,22
PRIMER_RIGHT_4=783,22
PRIMER_LEFT_4_TM=59.499
PRIMER_RIGHT_4_TM=59.775
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_05575 Ps_contig_mira-and-tgicl-ass_franssen_38642
SEQUENCE=AGTTGATTTGATGAACTCTTTTCTCTCTCCTCATCTTCAGAACTTAAAACACTCTGCAAACGAGCCCACCCTCCTCCGATCAACCTGCCGGAGAAGACGGTGGTTGCCGGCCAACGGCGGCGGCACCACCTTATTCTCCGGCGAACCACCACTCAAAAACCGGAACGTCAAATACTAACCCTACCTTGTTTTGGACGGAGAATCCAAATCCGGCCTTAACTTTTCCTAAATCCTCCTCAAACGACTGGATCGACCCTCCCAACCAGACGAAAATTCAAAACTCCGACGAAATAAACTTTCAACGCAACCTAACAAATCCTCGACTCCTTCTAACAGACAACTCAAAACAAAATCAACAGCAAGAAATAATTCAAACCCCAAACCCTAATTTCAAAATTAAAACCTCCCTAGTTGTAATTTACAAAAATCGTTAAAGGGGTTTTACTCAGTTCAGAACGACGAATATAACGACTTAAAGGTTTCAAGTAAAAGAGGGAGGTAAAGGCTACTTGGTCAGAGCGGATCCGACGGTGAGATATCCTTCGACGCGGTTCAGATAAGTCCTTTCTCCGAACTTTCTCCTTCTTCTTCTTCTCTGCTCTTCTCTGTTTGCTATTAGTTTCTGTGTTGGTCTGAGCTCATCGACGTTAGAATTTCAGCGTGAAACTCTAATGTAGATTTAAAAAAATCGTGAGCTTTTCTGAAAGGTGTTCTTGGAAATTTTTTGCAGAATAACGGTCCCCCCTCTTCAATGATTGAACTCCCTCTCTTATAGATGGGTGCATGTGGTTCTTTCGATTGGGAGCTGGCAGGGATTATAGGATCATTCCGTTAGATTTTCATCTCCCCGCGATTTGGTGAATCTTGCCAATCTGTTAGGTTATGCACAAGTCGTCTTGGTGCGAGCGCGAGGAGGATGACGATAAAGTGACTTGCACACTTAGCATGAATTTAATTCAATGTAAGCTATGGTCCTGGTTCGACACACTCTCTCTAGTTGGATGGATTCAAGTTACACGACATTTTATTTTGGTCAAAACTTGGTTTTGCAATTTGTAATTAAATATCATACCTATTCCTAATCATGATTTTGGAACAGAAACGTGTATAATTTCCCATTCATATGTATGGCTCTAAATTTGTGAAAAGAAAGAACTTGAATTGAACAA
TARGET=582,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4331
PRIMER_LEFT_PENALTY=0.347129
PRIMER_RIGHT_PENALTY=0.085951
PRIMER_LEFT_SEQUENCE=GGAGGTAAAGGCTACTTGGTCA
PRIMER_RIGHT_SEQUENCE=ATGAGCTCAGACCAACACAGAA
PRIMER_LEFT=494,22
PRIMER_RIGHT=641,22
PRIMER_LEFT_TM=59.653
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=0.4460
PRIMER_LEFT_1_PENALTY=0.360050
PRIMER_RIGHT_1_PENALTY=0.085951
PRIMER_LEFT_1_SEQUENCE=GGGAGGTAAAGGCTACTTGGTC
PRIMER_RIGHT_1_SEQUENCE=ATGAGCTCAGACCAACACAGAA
PRIMER_LEFT_1=493,22
PRIMER_RIGHT_1=641,22
PRIMER_LEFT_1_TM=60.360
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.4549
PRIMER_LEFT_2_PENALTY=0.347129
PRIMER_RIGHT_2_PENALTY=0.107763
PRIMER_LEFT_2_SEQUENCE=GGAGGTAAAGGCTACTTGGTCA
PRIMER_RIGHT_2_SEQUENCE=CCTTTCAGAAAAGCTCACGATT
PRIMER_LEFT_2=494,22
PRIMER_RIGHT_2=707,22
PRIMER_LEFT_2_TM=59.653
PRIMER_RIGHT_2_TM=59.892
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=214
PRIMER_PAIR_PENALTY_3=0.4678
PRIMER_LEFT_3_PENALTY=0.360050
PRIMER_RIGHT_3_PENALTY=0.107763
PRIMER_LEFT_3_SEQUENCE=GGGAGGTAAAGGCTACTTGGTC
PRIMER_RIGHT_3_SEQUENCE=CCTTTCAGAAAAGCTCACGATT
PRIMER_LEFT_3=493,22
PRIMER_RIGHT_3=707,22
PRIMER_LEFT_3_TM=60.360
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=0.5033
PRIMER_LEFT_4_PENALTY=0.347129
PRIMER_RIGHT_4_PENALTY=0.156135
PRIMER_LEFT_4_SEQUENCE=GGAGGTAAAGGCTACTTGGTCA
PRIMER_RIGHT_4_SEQUENCE=ACGCTGAAATTCTAACGTCGAT
PRIMER_LEFT_4=494,22
PRIMER_RIGHT_4=661,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=60.156
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=168
=
PRIMER_SEQUENCE_ID=SSR_05576 Ps_contig_mira-and-tgicl-ass_franssen_38654
SEQUENCE=TTATCRGGATACAAGGAGAGAGGGAGAGAGAAAGATAAAGAAGAAGAGAGAGAGAAAGGGAAAAAAGTAGAGAGAGAAAGAACCGTTTGAGATTTTTGGAGACGTTACCGGACTTCAAATCTTCAATTCACCGGAATTTGGGAACTACGGTCACGTCCGGAGACTTAGGGTTTTCCTTTTCTCTTGTTTGTATTTCAATTTCGGTCGTTTTCGATTTACGAAGTTTCGCGATGGGGTTACGAAGTTAAACGACGAATTCGTGATTTGAAGTTTCGTTGTTGTTGAGTTGAGAGTTTCGTTTCGGTGGTGATGTTTACGCCGCAGAGATTGTGGCCGGGTCGGTCCATAACGCCGAACAAGAGTGGAGCGCGCACTGGAACCGGGTCGGGTCTTGATTCGGGCGAGGGTGCTGGTTCGAAAGGGAAAGGTGTTGCTTTTGCTGAAAATGGTGGAAATTTGGATCGTGAGGTTTTGGTTGAGAGGGTTTCAAGCCTTGAAAAAGAGCTTTACGAGTACCAATTCAATATGGGGCTTCTCTTGATTGAGAAAAAGGAGTGGAACTCTAAGTTTTCCGAGCTAAATCAAGATTTAGTGGAAGTCAAGGATGCTTTTGAACGAGAAAAAGCAGCTCATTTATTTGCTCTATCTGAGGCAGAGAAGCGAGAAGAGAATCTGAGGAAGGCCTTTGGTGTTGAGAAAGAGTGTGTGCTTGATCTTGAGAAGGCCCTGCGAGAAATGAGGTCAGAGCATGCGAAGACTAGATTTGCAGCAGACTCAAAGTTAGCTGAAGCAAATGCTTTAATAGCTAGTGTTGAAGAAAAATCTTTAGAAGTAGAAGCCAAGTTACGCTCTGCTGATGCCAAACTTGCTGAAATTAGCAGAAAGAGTTCAGAGATTGATAGGAAATCACATGACTTGGAGGCTCAAGAATCTGCACTTAGAAGGGAGCGATTGTCCTTCATTGCAGAGCAAGAATCACACGAGAGTACTTTGTCTAAGCAAAGAGAGGACTTACGAGAGTGGGAGAAGAAACTGCAGGATGGCGAAGAGAGGCTGTCCAAGGGTCAAAGAATTCTCAATGAAAGAGAGCAGAGGGCTAATGAGGTTGACAGAATATGTAGGCAGAAAGAGAAGGACCTTGAAGAGGCACAAAAGAACATTGATGCGGCTTATGTAACATTGAGAGGCAAAGAAGATGATGCAAATAGCAGGCTGGCAACTATAGCTCTCAATGAGAAGGAATGTGATTCTATGAGAACGAACTTGGATCTCAAAGAGAAGGAGTTATCTGCATGGGAGGAAAAGCTCAATGCCAGAGAAAAAGTTGAGATTCAGAAGCTTGTTGATGAACACACTGCTATTCTTGATGTGAAGAAGCAAGAGTTTGAGGTGGAATTGGTTAGAAAAAAGAAAGTCATTTGAAGATGGATTGAGGAATAGATTGATAGAAGTGGAAAAGAAAGAAGGTGAAGTCAATCACAAGGAGGAGAAGATTGCAAAACGGGAGCAAGCCTTGGAAAAGAAAGCAGAGAAGCTCAAGGAGAAAGAGAAAGAATATGAAGTAAAAGTCAAAGCTTTGAAGGAACGGGAGAAGTCTATTAAGTCTGAGGAAAAAGACTTAGGGAAGGAGAGGGGTGAAATAGAAAGTGAGAGAGAAGAGTTGCTCAGTCTCAAGGCTGAAGTTGAGAAATTAAAGGCAAACAACGAAGAAGAATTGCTGAAAATTAAAGAAGAGACTAATCATCTTCAAGTGACTGAGGAGGAGAGGTCAGAATATATACGTTTGCAGTCGCAACTGAAGCATGAAATTGATCAGTATAGGCTTCAGAAAGAACTACTGATGAAGGAAGCTGATGACTTGAGGCAGACAAAAGGAGACTTTCGAAAGAGAATGGGATGAGCTTGATCTGAAAAGAGCAGATGTGGAGAAAGAGCTGAAAAATTTGCTTCAACAGAAGGAAGAAANNNNRAACTACAACAAATGAAGAAGAG
TARGET=45,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05577 Ps_contig_mira-and-tgicl-ass_franssen_38665
SEQUENCE=TTATCTCAGTTAACAGTGAACAAGGAAGGAAGGAGCCAGCTTTTAGACACGGAATACAGAAATAAGATTCTCGATGATCTCTATGAGATGAAATCCTTCTTAAATCAACGGTTAGCTGAGTTGAAGAATGAGGAAACTTTGTCGCTACAAAATCAAGTACAGGCAGTTTCTCCTTTTGTACTACAGCAGTATGCTGCTGATACTATAGACACTATGCAAAGTGATATTTCTTTGGCAATTTCATTGCTTACAAATAGGAAGACAAGGGATCTGATTATGATTCTCAACTCCAAAAGATTTCTAGACAGACTAGTGAACTCTTTGGAGGAAAAGAAACATCATGAAGTCAAGTTGGAAGAGGGGCTAAAGGACTTGGCTACTAAGCGCATGGAGCTACAAAATTCATTATCTTCATTGTGGCCAAAACAAGACGCTGCCGTGGCTAAAACAAAGGAACTGAAAAAACTGTGTGAGAACACACTTTCATCCATGTTTGATGGAAGACCAGTTAATATAATTGGAGAGATCAACACCATTCTGACCAGTGGTCTCGGAGCATGAAGATCTGATTGAAGAAATGGAGCTTTCAAGTGGATAATGACTAAAGTTTTAGTAACAGAAAAGTGGGTTTTAAGGTGTAGAGCCTTTTCCTGCTATGTTTTGTTTTTATTTTCACATTTCCGGTTTCTTTTTTTGACGTAAACATTACTTTTATAATGCAAATGATCCATAGTTATAATTCTTTCCCGACTTGTTATAAATGAAGCAACCTTGGTAATTTGTCATTTGAAACCGCGTCCTTATTGAT
TARGET=22,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05578 Ps_contig_mira-and-tgicl-ass_franssen_38666
SEQUENCE=AAGAAATGGTTCAGTAAAGACGACATTCCAAAACTATACAGCAAAGCATCATCAGCGTCCAGGTTCATCCCCCAACCCTTCAAACTCGCTTCTTCGTATTAGGGCTATTAGGGTTAGGGTTTTCTTCTTCTTCTTCTTCCTCACTTCTTCAAATTCTTCTCCTTCCGCTAAATCTCAATGTCCTATTCTTCAATTTTCCTCACACRATCTCTACTAAACCATCACTGCAACTGTAAAATCTATCGTCAATTCCTCTATTGTTGTTGTTAATCGATTTCATTGCTCCGTTTCTTCCATTTCACAAACTGTTTTNGAT
TARGET=122,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05579 Ps_contig_mira-and-tgicl-ass_franssen_38668
SEQUENCE=AGTTTTAACCTATTCCAATCATCTGATTCACTAAAACAAAAAAGTTAAAAACCACTTGCCACAATTACATGAAAAGAGAAGTCAAAATCAAGATTCTTGATATCCAATGTCAAATAAACAGTTAACATGGACACTAAAGCAGAATCAAAAATACTAACAAGAAAAACTTAAAAATGAAATTCAAGCAATAAAGTGAAACACGATATGGTCCTATTGATTAACAGAAAGCTCCCTTCGCTTGTATCGGACCATAACTTTTCCTTTCACTCCGACAACCATGGCATTCCAATCAATCTCGGGGAAAGGTGACAGAAGCTCTAGTTCAAAGTTTCTAAGAAGATGAGCCCAAATTGCTTTTATCTGGAGATATGCAAAAGGCTCTCCGAGGCATCCATGTCTACCACCTCCAAAGGAAATGTAAGAGAATGCCCCAGCTGCCTTGTCTTCTTCCCTCCCTACAGCATACCTATCAGGATCATACTTATCAGGGTCATTGAAAATGTGAGGTAACCTGTTTGCAAATGCAGGGGAAGTAGCAACTATATGACCCTTGGGAATGTCATACTCTTTCCCTTCTCTTGTAGTGACGCTAAAATCGCTGTGTGAGCTTCGAAGCAGCATAATCAATGGAGGGTGGAGTCTCAAGGCTTCTTTAATGCACCTATACAAGACGTCCATCTCGGCCAAAACATCGTGATCAACTCTATCGCCGTGTTTCGCCATCAAGTTCTTCTGCTCCTCCACTACACCAGAAAGGTACTGTTTGTTACACAGGAGATATGCTCCAGTCCAAGTTGATGTTATTGAACTGGTGTGCTGCCCCGCGAAAAGAGCAGCAATGAGGAGACCGGTGACTTCGGCTTCTGTTGTAGGGCGACCGTCTTTGTATTTTGAGTCAATGAAGCACTGCAGCATGTCGTCCTCTGATTTGTTGGAACCTTTTCGTGAAGATATAATGTTTGCAAAGATTTCAGCAAGCTTCTTGCGTGCATTGTCACGGCGTCTGTGAGCTGGGATTGGTAGGTATGGGAAGAGAACACTGATTGGAAGCATTCCGTTGTCGAGGTCGTGGAACAATGCAGAGACATCATCAAAGAGCTTGTCACGAACTTCACGCCCCAACAGACATCTACTGGCTGTCAAGATGATCAGATGCTCAAGTTCATACTTCAAATCAACTTCACCACTTGATCCCCATTTTGAGAAATAGTCCTCAGCTTCAGAAACCATCTGATTAACATAACTCTTGAGTTTATTAACCCTAAGCGCTTCAGTAAAAAACCTAAACTGTTCCTGCCTAACAGAATAATCAACATCAAAAACAACACCAGGACCAAAAGTCGGCACATTAAACTGATAAACCTCTTGCTGACTAAGATCAGTTTCAGGAGCTTTGAAAAAATGCGCAGAAACCTCAGGACCAATCAAAAAGGTGATATTTTTATGAAAGATTTTCAAGGTGAAAACGCCACCGAGTTTTGGGTATTCTTCACGGAGCATAAAGATTGGACCTTTGAGGAAACGGAGGAGTCCTCCGATAATGGGCCATCCTGGAAGAATTGGGGGTAGGCGTTTTTTAGATTTGGGGATGATGAAATATGAAATGAGTTTTGCTACGATTATGGTTGTGATGAGGAGAAGAACAGTGTTGAGGAACTTGTTTCCATCAAAAACATCCATGATTGATGATGATGGTTGTGAGTGTAATTAGATCTAGAATATTGAGAGATCTAAGAACAAGTGCATTATTATTATTATAGTGATGAGTGATTGTGGATATTAGTGAGAACAGAAAGGAATCCCCNNGTAAAGATTATTTGTAGATTTGTTTATAAGGGTGGATTTGGATTTGG
TARGET=1745,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6713
PRIMER_LEFT_PENALTY=0.580157
PRIMER_RIGHT_PENALTY=1.091174
PRIMER_LEFT_SEQUENCE=TTGATGATGATGGTTGTGAGTG
PRIMER_RIGHT_SEQUENCE=CCAAATCCAAATCCACCCTTAT
PRIMER_LEFT=1682,22
PRIMER_RIGHT=1852,22
PRIMER_LEFT_TM=59.420
PRIMER_RIGHT_TM=61.091
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=171
PRIMER_PAIR_PENALTY_1=1.7752
PRIMER_LEFT_1_PENALTY=0.580157
PRIMER_RIGHT_1_PENALTY=1.195027
PRIMER_LEFT_1_SEQUENCE=TTGATGATGATGGTTGTGAGTG
PRIMER_RIGHT_1_SEQUENCE=GGGGATTCCTTTCTGTTCTCAC
PRIMER_LEFT_1=1682,22
PRIMER_RIGHT_1=1803,22
PRIMER_LEFT_1_TM=59.420
PRIMER_RIGHT_1_TM=61.195
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=122
PRIMER_PAIR_PENALTY_2=1.8331
PRIMER_LEFT_2_PENALTY=0.547508
PRIMER_RIGHT_2_PENALTY=1.285616
PRIMER_LEFT_2_SEQUENCE=CGATTATGGTTGTGATGAGGAG
PRIMER_RIGHT_2_SEQUENCE=GGGATTCCTTTCTGTTCTCACT
PRIMER_LEFT_2=1616,22
PRIMER_RIGHT_2=1802,22
PRIMER_LEFT_2_TM=59.452
PRIMER_RIGHT_2_TM=58.714
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=1.8787
PRIMER_LEFT_3_PENALTY=0.683672
PRIMER_RIGHT_3_PENALTY=1.195027
PRIMER_LEFT_3_SEQUENCE=ACGATTATGGTTGTGATGAGGA
PRIMER_RIGHT_3_SEQUENCE=GGGGATTCCTTTCTGTTCTCAC
PRIMER_LEFT_3=1615,22
PRIMER_RIGHT_3=1803,22
PRIMER_LEFT_3_TM=59.316
PRIMER_RIGHT_3_TM=61.195
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=1.9042
PRIMER_LEFT_4_PENALTY=0.813021
PRIMER_RIGHT_4_PENALTY=1.091174
PRIMER_LEFT_4_SEQUENCE=GGTTGTGATGAGGAGAAGAACA
PRIMER_RIGHT_4_SEQUENCE=CCAAATCCAAATCCACCCTTAT
PRIMER_LEFT_4=1623,22
PRIMER_RIGHT_4=1852,22
PRIMER_LEFT_4_TM=59.187
PRIMER_RIGHT_4_TM=61.091
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_05580 Ps_contig_mira-and-tgicl-ass_franssen_38670
SEQUENCE=ACATCATAAGCAAAAGTCAACTAAACCAAACCTAAACTACCTTAACTATTATCCAAATCATGTTGGTATGGTATTGGTAAAGAAAGTAATTAACAACTTTAATAACAAAAACAAAAACAAAACAAAAAACATGTTTTTTCCAAATTCCAACATAAATAAACCATATATATAATAATAATATCATATATTAAAAACAAAGTTCTTTTTGTTCTTCTCTTCTTCTTCATGTGTTCTTCATTGTTTTGTTCTTCCTCTAAGGAGTAAAGGAAGTGTTATTATTACCAAGAGTTGATCCTAGATGATCATTGCACAAACTAGCTCCTTCTTGAGTTGTTTTGTTGTTTTCGGCCCTTGGAGAACTTCCGGCACTACTATTGGAAAGTGATCCGAATGCTGTTTTTTGAAGGACGCCGGTTGGTGATGATCCCATCGGTGGACTATTGTGATTGTCCCAACCATCAGTCATGAGATTTAGAGCCGAGGAATTGTTGTTGTTGATGTTGTTACTCCCGTAATGATCATCGCTTGCATTGCAAGCAATGGTGCTAAGATGAAGTACTTCGCCGAGTGGACCACCCATTGAGCTCTCCCAAGTTATTGGAATCCAATTAGCTTGCCTTGTGGCAGGATTATTAGTACTAGGGTTTGATGATTCGTTGTTATTGGGAGTACTATTTCCGACGCCTAACCCCATTTGAATAGGGTCGATTTCCCTTGAGAGCCTAAGAGGCGAAAGTGTGAGTTTTTCATTGGTGGCCGAGGAAGGGAACGACATGAAATCCGAGGCAGTCATTGGTGTTGAGATTGAAAGCTGTGTCCTATCCGACTGCATGTCAAGTTCCGGCCAAGAAAAACCACCCGAGCGGTTATGATGATTGTGATGATCAGAGTGAGTTTTGGGAAAGTCGTCGATGAAATGCCTGAGTGAATGTTGAGAGCCTGTGTCACGGTCTGTAGGGTTCTGTGAAGCTGAACCAAAGTTTCTGGTTCCAAGCAAGGTAGTGGTTTTCTGTGAAGGGTTAAGCAGGGAATCGGAAGTGACAAACCCGAATTCATTGTTCCTTGAAGATTCATCATAAGGAATGTGCTGTTTTTGAATCATGAAGGAATTATTGTCCTTCGGTTTCAACTCAAGGTTGGTGGCAGCATCGGTAGTGCCGAACCGTCCTGCATCCTCTCACTATTTGTTTGTTATTCTCTTTGCTATTCATGAACATCCTGTTGATGGTGTTGGCAGCAGAAGAAGCATTATCTTGTCCAATAAGGCTGTTCATATTCTTAGAATGTTCCTGATGTGCAAAGGAAAGAGTGTTGGTAGCGTTGTTGCCACCGGGAACAACAATAGACGAAGAAGGAGAAGCAGCGACATTGATTTGCTTCGACGAACCACTTGGAGTAGTAATGGTAGAAGAAACATTAGTAGTAGCTGTATTTGTGGTTCCACCAGTGGTGAGAGCCTGGCCCGATTGGCCTTCCACAGGCTTTCTTGAACGATGGCGTCCTCTGTTCATATGCCTTT
TARGET=169,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05581 Ps_contig_mira-and-tgicl-ass_franssen_38670
SEQUENCE=ACATCATAAGCAAAAGTCAACTAAACCAAACCTAAACTACCTTAACTATTATCCAAATCATGTTGGTATGGTATTGGTAAAGAAAGTAATTAACAACTTTAATAACAAAAACAAAAACAAAACAAAAAACATGTTTTTTCCAAATTCCAACATAAATAAACCATATATATAATAATAATATCATATATTAAAAACAAAGTTCTTTTTGTTCTTCTCTTCTTCTTCATGTGTTCTTCATTGTTTTGTTCTTCCTCTAAGGAGTAAAGGAAGTGTTATTATTACCAAGAGTTGATCCTAGATGATCATTGCACAAACTAGCTCCTTCTTGAGTTGTTTTGTTGTTTTCGGCCCTTGGAGAACTTCCGGCACTACTATTGGAAAGTGATCCGAATGCTGTTTTTTGAAGGACGCCGGTTGGTGATGATCCCATCGGTGGACTATTGTGATTGTCCCAACCATCAGTCATGAGATTTAGAGCCGAGGAATTGTTGTTGTTGATGTTGTTACTCCCGTAATGATCATCGCTTGCATTGCAAGCAATGGTGCTAAGATGAAGTACTTCGCCGAGTGGACCACCCATTGAGCTCTCCCAAGTTATTGGAATCCAATTAGCTTGCCTTGTGGCAGGATTATTAGTACTAGGGTTTGATGATTCGTTGTTATTGGGAGTACTATTTCCGACGCCTAACCCCATTTGAATAGGGTCGATTTCCCTTGAGAGCCTAAGAGGCGAAAGTGTGAGTTTTTCATTGGTGGCCGAGGAAGGGAACGACATGAAATCCGAGGCAGTCATTGGTGTTGAGATTGAAAGCTGTGTCCTATCCGACTGCATGTCAAGTTCCGGCCAAGAAAAACCACCCGAGCGGTTATGATGATTGTGATGATCAGAGTGAGTTTTGGGAAAGTCGTCGATGAAATGCCTGAGTGAATGTTGAGAGCCTGTGTCACGGTCTGTAGGGTTCTGTGAAGCTGAACCAAAGTTTCTGGTTCCAAGCAAGGTAGTGGTTTTCTGTGAAGGGTTAAGCAGGGAATCGGAAGTGACAAACCCGAATTCATTGTTCCTTGAAGATTCATCATAAGGAATGTGCTGTTTTTGAATCATGAAGGAATTATTGTCCTTCGGTTTCAACTCAAGGTTGGTGGCAGCATCGGTAGTGCCGAACCGTCCTGCATCCTCTCACTATTTGTTTGTTATTCTCTTTGCTATTCATGAACATCCTGTTGATGGTGTTGGCAGCAGAAGAAGCATTATCTTGTCCAATAAGGCTGTTCATATTCTTAGAATGTTCCTGATGTGCAAAGGAAAGAGTGTTGGTAGCGTTGTTGCCACCGGGAACAACAATAGACGAAGAAGGAGAAGCAGCGACATTGATTTGCTTCGACGAACCACTTGGAGTAGTAATGGTAGAAGAAACATTAGTAGTAGCTGTATTTGTGGTTCCACCAGTGGTGAGAGCCTGGCCCGATTGGCCTTCCACAGGCTTTCTTGAACGATGGCGTCCTCTGTTCATATGCCTTT
TARGET=486,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2853
PRIMER_LEFT_PENALTY=0.228464
PRIMER_RIGHT_PENALTY=0.056850
PRIMER_LEFT_SEQUENCE=GTCATGAGATTTAGAGCCGAGG
PRIMER_RIGHT_SEQUENCE=ACTCACACTTTCGCCTCTTAGG
PRIMER_LEFT=461,22
PRIMER_RIGHT=742,22
PRIMER_LEFT_TM=60.228
PRIMER_RIGHT_TM=59.943
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.2855
PRIMER_LEFT_1_PENALTY=0.021195
PRIMER_RIGHT_1_PENALTY=0.264300
PRIMER_LEFT_1_SEQUENCE=AACTTCCGGCACTACTATTGGA
PRIMER_RIGHT_1_SEQUENCE=TTCATCTTAGCACCATTGCTTG
PRIMER_LEFT_1=357,22
PRIMER_RIGHT_1=554,22
PRIMER_LEFT_1_TM=60.021
PRIMER_RIGHT_1_TM=60.264
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.2855
PRIMER_LEFT_2_PENALTY=0.021195
PRIMER_RIGHT_2_PENALTY=0.264300
PRIMER_LEFT_2_SEQUENCE=GAACTTCCGGCACTACTATTGG
PRIMER_RIGHT_2_SEQUENCE=TTCATCTTAGCACCATTGCTTG
PRIMER_LEFT_2=356,22
PRIMER_RIGHT_2=554,22
PRIMER_LEFT_2_TM=60.021
PRIMER_RIGHT_2_TM=60.264
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=0.4063
PRIMER_LEFT_3_PENALTY=0.021195
PRIMER_RIGHT_3_PENALTY=0.385098
PRIMER_LEFT_3_SEQUENCE=AACTTCCGGCACTACTATTGGA
PRIMER_RIGHT_3_SEQUENCE=ATAACTTGGGAGAGCTCAATGG
PRIMER_LEFT_3=357,22
PRIMER_RIGHT_3=597,22
PRIMER_LEFT_3_TM=60.021
PRIMER_RIGHT_3_TM=59.615
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=241
PRIMER_PAIR_PENALTY_4=0.4063
PRIMER_LEFT_4_PENALTY=0.021195
PRIMER_RIGHT_4_PENALTY=0.385098
PRIMER_LEFT_4_SEQUENCE=GAACTTCCGGCACTACTATTGG
PRIMER_RIGHT_4_SEQUENCE=ATAACTTGGGAGAGCTCAATGG
PRIMER_LEFT_4=356,22
PRIMER_RIGHT_4=597,22
PRIMER_LEFT_4_TM=60.021
PRIMER_RIGHT_4_TM=59.615
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_05582 Ps_contig_mira-and-tgicl-ass_franssen_38686
SEQUENCE=ATTCTTATAAAGAAAATACATGTTCACTTCTTCTCACCTTCCTAACCCTTTCCTTCTATCAAACCAAACCACTCTCATTCATCAACTCCTTATTAATTTCTTTTCTTGCTCTTGAATCAAAGGACCAAAATTTATCACCTTTTTTTTCCATCTAATTGCACACAAGAAAATCTTTATTTTAGAAGGTTGAACTTAAGATATGGGTTTGAGTTCTAAACAAGTTACTAGTAGAGGGATTGATTGGAACCAAACCTTGCTACAAGATCAAAACTTAGAACTACCAAAAGCTTCACCTATGAGAAAACAACAACAACAACAAAATCATCAATTAGAAGCTGTGAATTGTCCAAGATGTGATTCTTCAAACACCAAATTTTGTTACTACAACAACTACAACAAGTCTCAACCTCGTCATTTCTGCAGAGCTTGTAAAAGACATTGGACTAAAGGTGGAACTCTAAGAAATGTTCCTGTTGGTGGTGTTAGAAAGAATAAGAGAGTCAAAAAATCAACCTCTCCAAAAACTACTATTAGCACCTCCTCAAAAACTACTATTACCACCCCCACCCCCACCACCTCCAATAGCAAAACCACACCTTCTATGTATGAATCTATCATTCATNCNCCTNTCTTTTTTCCATCTCAGAAT
TARGET=303,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3132
PRIMER_LEFT_PENALTY=0.122569
PRIMER_RIGHT_PENALTY=0.190632
PRIMER_LEFT_SEQUENCE=GATTGGAACCAAACCTTGCTAC
PRIMER_RIGHT_SEQUENCE=TGACGAGGTTGAGACTTGTTGT
PRIMER_LEFT=238,22
PRIMER_RIGHT=413,22
PRIMER_LEFT_TM=59.877
PRIMER_RIGHT_TM=59.809
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.5667
PRIMER_LEFT_1_PENALTY=0.122569
PRIMER_RIGHT_1_PENALTY=0.444122
PRIMER_LEFT_1_SEQUENCE=GATTGGAACCAAACCTTGCTAC
PRIMER_RIGHT_1_SEQUENCE=TCTAACACCACCAACAGGAACA
PRIMER_LEFT_1=238,22
PRIMER_RIGHT_1=486,22
PRIMER_LEFT_1_TM=59.877
PRIMER_RIGHT_1_TM=60.444
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=249
PRIMER_PAIR_PENALTY_2=0.5676
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.559375
PRIMER_LEFT_2_SEQUENCE=CAAACCAAACCACTCTCATTCA
PRIMER_RIGHT_2_SEQUENCE=CACATCTTGGACAATTCACAGC
PRIMER_LEFT_2=59,22
PRIMER_RIGHT_2=355,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.559
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=0.6108
PRIMER_LEFT_3_PENALTY=0.122569
PRIMER_RIGHT_3_PENALTY=0.488216
PRIMER_LEFT_3_SEQUENCE=GATTGGAACCAAACCTTGCTAC
PRIMER_RIGHT_3_SEQUENCE=TTTCTAACACCACCAACAGGAA
PRIMER_LEFT_3=238,22
PRIMER_RIGHT_3=488,22
PRIMER_LEFT_3_TM=59.877
PRIMER_RIGHT_3_TM=59.512
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=0.6710
PRIMER_LEFT_4_PENALTY=0.122569
PRIMER_RIGHT_4_PENALTY=0.548390
PRIMER_LEFT_4_SEQUENCE=GATTGGAACCAAACCTTGCTAC
PRIMER_RIGHT_4_SEQUENCE=AACACCACCAACAGGAACATTT
PRIMER_LEFT_4=238,22
PRIMER_RIGHT_4=483,22
PRIMER_LEFT_4_TM=59.877
PRIMER_RIGHT_4_TM=60.548
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_05583 Ps_contig_mira-and-tgicl-ass_franssen_38704
SEQUENCE=CGAAAGCAGNGGTAGTCAACGCAGATACGGGGAAGGCAGCAGTAAAGTACGCGAAAGAAGCCATGGCTTATGCCTTTTCYTCATCAGTGGAGAGCAAACTCTAGTCAGAGCCAAGTACTAGGCAGTTACGAGCTCGGCAGAGCTAACTGGGGTTGGAGCTGGAAGGAACGCTGGATCGCCGCGCGTCCATGGGAAAGCCGTGTAGCTAGCATTAGCCCGAAGAAAGCTCAAAATAAACCATCATTCAAAGTTCAAAAAGATAAAAAGACATCAACATCTAAAACTCCGGTTTCGGTAACACCTCCTTCCTCCAGTGCAAAAGGGACTCCTCCTCCTCCTAAAGGAAGTACCAAAGCTAGAAGATTGTCTTTTCCAACCACAGAGAAAGAGAAAACTGTGGTTGAAGGAGGAGTGAAGTGAGTATTCAACAATGGTTCCTAAGAAAGAAAAGCTTTAAATTTTCAAGTAATAAAGTCCTTTTGTATTAATTTGAGATCTACTTTTATTTATTCAGTTGGTATTTGTCCTTTAACTTATTTTGT
TARGET=326,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05584 Ps_contig_mira-and-tgicl-ass_franssen_38708
SEQUENCE=GCTAGATGTCACAAACATGAAAAGTATATTTATATTTCAAAAGCTAAATATACATCAAGTTAGCTTCAATTTGAAACACAAGAAGCAACCTCAAAGTAAAAATAAAAACAAGTACCAAAACAAAATAGTCTTTAAACAAACAAATTAAAAATCAAAACCACCATCAAAGCCACCAGCATCAAGGCCATCATCATATCCAGCATCATAAGCACCACCATCAGACACCATATCACCAATCAACATCCCACCTAATGCACCTCCAAGCAACCCTGCTCCCAAACCCATTCCAAATTTATTCTTCTTAGGCTTCTGTGCCCCTGGATATCCATAACCCGGTTGTTGTTGTGGTGGATATCCATAGCCTTGTTGTTGTTGTTGTTGTGGTGGATATCCATAGCCTTGTTGTTGTGGTGGAGGATATCCATAGCCCGGTTGTTGTTGTTGTTGGGGTGGATAGCCATAACCGGTTTGAGCAGGTGGGTATCCACCGTAAGCAGTCGCCGGTGGTGGATACCCATATCCAGCCGCTTGTTGCGGTGGTGGATACGGTGTACCGTACGGAACAGAACTAGACCCTGCCGCCACTCCGGGTGGAGGATACGCCAACACTGGTTCATCTTTCCCCATCTTTGAGTCATGTTTCCCTTTCACCTCTGACTTGGTCGCCGGAGCTCCAACATTTTCGCCGAATTTGTAGGAGAGATTCAAACTCCCCTTGGATTTCCCTGAGGTTGTCCTAACCTGATAACTAACTTGCCTTAAAGAATCCCCGGAAGTGTTCTCAAGAAGTTCCTTGAGAGGCATATGAACAGTACCGATCAAGGTATCTCCGGCAACAGATCGATTTGAGATGAGTTTAATCTCAAGAGAGAGACGATTCTGATTAGCAAGAGATTCATTGACGGTGAATTTGATCGGGAAATTCCAGGTAGGGTTTGTTCCGCCGTTATGGTGGATATGGGTGGTGGCAGTCTGTGGGTTCACAGGATCTCCGGAGATTGAAACGACAGCATATACGTCCATCTTGGAGAAGAGATTAACATCTTTGAGATCTTTTGCAGAAATTATGTTGAGTTCTAGGGTTTTATATTCCATGGAAATCAACAGAAAAGAGAGAAATTTTGATGAATGATTTGTGTGTATAATAAAGTTTGTGATGTTTCGATCNAGTACTCTGCGTTGATCCACTG
TARGET=364,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1204
PRIMER_LEFT_PENALTY=0.011696
PRIMER_RIGHT_PENALTY=0.108684
PRIMER_LEFT_SEQUENCE=TATCCAGCATCATAAGCACCAC
PRIMER_RIGHT_SEQUENCE=TATCCTCCACCACAACAACAAG
PRIMER_LEFT=193,22
PRIMER_RIGHT=419,22
PRIMER_LEFT_TM=59.988
PRIMER_RIGHT_TM=59.891
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.2275
PRIMER_LEFT_1_PENALTY=0.118777
PRIMER_RIGHT_1_PENALTY=0.108684
PRIMER_LEFT_1_SEQUENCE=ACCATCAGACACCATATCACCA
PRIMER_RIGHT_1_SEQUENCE=TATCCTCCACCACAACAACAAG
PRIMER_LEFT_1=213,22
PRIMER_RIGHT_1=419,22
PRIMER_LEFT_1_TM=60.119
PRIMER_RIGHT_1_TM=59.891
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.2275
PRIMER_LEFT_2_PENALTY=0.118777
PRIMER_RIGHT_2_PENALTY=0.108684
PRIMER_LEFT_2_SEQUENCE=CACCATCAGACACCATATCACC
PRIMER_RIGHT_2_SEQUENCE=TATCCTCCACCACAACAACAAG
PRIMER_LEFT_2=212,22
PRIMER_RIGHT_2=419,22
PRIMER_LEFT_2_TM=60.119
PRIMER_RIGHT_2_TM=59.891
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.2275
PRIMER_LEFT_3_PENALTY=0.118777
PRIMER_RIGHT_3_PENALTY=0.108684
PRIMER_LEFT_3_SEQUENCE=CCACCATCAGACACCATATCAC
PRIMER_RIGHT_3_SEQUENCE=TATCCTCCACCACAACAACAAG
PRIMER_LEFT_3=211,22
PRIMER_RIGHT_3=419,22
PRIMER_LEFT_3_TM=60.119
PRIMER_RIGHT_3_TM=59.891
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.2275
PRIMER_LEFT_4_PENALTY=0.118777
PRIMER_RIGHT_4_PENALTY=0.108684
PRIMER_LEFT_4_SEQUENCE=ACCACCATCAGACACCATATCA
PRIMER_RIGHT_4_SEQUENCE=TATCCTCCACCACAACAACAAG
PRIMER_LEFT_4=210,22
PRIMER_RIGHT_4=419,22
PRIMER_LEFT_4_TM=60.119
PRIMER_RIGHT_4_TM=59.891
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_05585 Ps_contig_mira-and-tgicl-ass_franssen_38708
SEQUENCE=GCTAGATGTCACAAACATGAAAAGTATATTTATATTTCAAAAGCTAAATATACATCAAGTTAGCTTCAATTTGAAACACAAGAAGCAACCTCAAAGTAAAAATAAAAACAAGTACCAAAACAAAATAGTCTTTAAACAAACAAATTAAAAATCAAAACCACCATCAAAGCCACCAGCATCAAGGCCATCATCATATCCAGCATCATAAGCACCACCATCAGACACCATATCACCAATCAACATCCCACCTAATGCACCTCCAAGCAACCCTGCTCCCAAACCCATTCCAAATTTATTCTTCTTAGGCTTCTGTGCCCCTGGATATCCATAACCCGGTTGTTGTTGTGGTGGATATCCATAGCCTTGTTGTTGTTGTTGTTGTGGTGGATATCCATAGCCTTGTTGTTGTGGTGGAGGATATCCATAGCCCGGTTGTTGTTGTTGTTGGGGTGGATAGCCATAACCGGTTTGAGCAGGTGGGTATCCACCGTAAGCAGTCGCCGGTGGTGGATACCCATATCCAGCCGCTTGTTGCGGTGGTGGATACGGTGTACCGTACGGAACAGAACTAGACCCTGCCGCCACTCCGGGTGGAGGATACGCCAACACTGGTTCATCTTTCCCCATCTTTGAGTCATGTTTCCCTTTCACCTCTGACTTGGTCGCCGGAGCTCCAACATTTTCGCCGAATTTGTAGGAGAGATTCAAACTCCCCTTGGATTTCCCTGAGGTTGTCCTAACCTGATAACTAACTTGCCTTAAAGAATCCCCGGAAGTGTTCTCAAGAAGTTCCTTGAGAGGCATATGAACAGTACCGATCAAGGTATCTCCGGCAACAGATCGATTTGAGATGAGTTTAATCTCAAGAGAGAGACGATTCTGATTAGCAAGAGATTCATTGACGGTGAATTTGATCGGGAAATTCCAGGTAGGGTTTGTTCCGCCGTTATGGTGGATATGGGTGGTGGCAGTCTGTGGGTTCACAGGATCTCCGGAGATTGAAACGACAGCATATACGTCCATCTTGGAGAAGAGATTAACATCTTTGAGATCTTTTGCAGAAATTATGTTGAGTTCTAGGGTTTTATATTCCATGGAAATCAACAGAAAAGAGAGAAATTTTGATGAATGATTTGTGTGTATAATAAAGTTTGTGATGTTTCGATCNAGTACTCTGCGTTGATCCACTG
TARGET=432,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0798
PRIMER_LEFT_PENALTY=0.019966
PRIMER_RIGHT_PENALTY=0.059875
PRIMER_LEFT_SEQUENCE=GCCTTGTTGTTGTTGTTGTTGT
PRIMER_RIGHT_SEQUENCE=GACTCAAAGATGGGGAAAGATG
PRIMER_LEFT=360,22
PRIMER_RIGHT=635,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=59.940
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.0798
PRIMER_LEFT_1_PENALTY=0.019966
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=GCCTTGTTGTTGTTGTTGTTGT
PRIMER_RIGHT_1_SEQUENCE=ACTCAAAGATGGGGAAAGATGA
PRIMER_LEFT_1=360,22
PRIMER_RIGHT_1=634,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.0798
PRIMER_LEFT_2_PENALTY=0.019966
PRIMER_RIGHT_2_PENALTY=0.059875
PRIMER_LEFT_2_SEQUENCE=GCCTTGTTGTTGTTGTTGTTGT
PRIMER_RIGHT_2_SEQUENCE=ATGACTCAAAGATGGGGAAAGA
PRIMER_LEFT_2=360,22
PRIMER_RIGHT_2=637,22
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=59.940
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.0798
PRIMER_LEFT_3_PENALTY=0.019966
PRIMER_RIGHT_3_PENALTY=0.059875
PRIMER_LEFT_3_SEQUENCE=GCCTTGTTGTTGTTGTTGTTGT
PRIMER_RIGHT_3_SEQUENCE=TGACTCAAAGATGGGGAAAGAT
PRIMER_LEFT_3=360,22
PRIMER_RIGHT_3=636,22
PRIMER_LEFT_3_TM=59.980
PRIMER_RIGHT_3_TM=59.940
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=277
PRIMER_PAIR_PENALTY_4=0.1657
PRIMER_LEFT_4_PENALTY=0.019966
PRIMER_RIGHT_4_PENALTY=0.145769
PRIMER_LEFT_4_SEQUENCE=GCCTTGTTGTTGTTGTTGTTGT
PRIMER_RIGHT_4_SEQUENCE=AGTCAGAGGTGAAAGGGAAACA
PRIMER_LEFT_4=360,22
PRIMER_RIGHT_4=658,22
PRIMER_LEFT_4_TM=59.980
PRIMER_RIGHT_4_TM=60.146
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_05586 Ps_contig_mira-and-tgicl-ass_franssen_38709
SEQUENCE=TTTTAGNNATCCTTATGCTTCTCTGGCTAGAAAATTAAATGAATATCAGATACATTAATCATTGTCTTTGGTAATAATAATGAAATGATTAGAGATGATCATCATAATTGAAATGATTAGGAATCATTTGCATGGAAAAAGAAGGATTAAAAGAGAAAGCAATACTAGATGGATGATTACAATCCATAGTATTAACTTTGTTACCTAAACTTTCAAGTGCCTTAATTTGTGACCTTAAAAACTTGAGATAATTTGCAGCCTCATCAAGCATTGAAGCAGTGTCCATTTTGTTTCCACCAGGTACAATCTTTTGCAAAGCCCTAATCCTATCACTTATTTTTTCCCTTCTTTGTCTTGCAGCCACAGTTTGTGGATCGTTTGATATCTTAACATTCTTTCTCTTAGGCTTTTTTCAATCACATTGATGTCGTCGATTCCGAAATTCACTGGCCTAAATGCTGCAGCTCTATACATCATTTCCTTCATTTGCGCTATTGCTTCTCGATCAGGCTCTTCGGTTGAAGATGAACAGCATATTGATGATGATGACGATGAATTTGGTTGTTGGAAATTTATGTTGGTTGAACAAGGTGCTTTGTCAGATCTTGGTTTCTTGTTTGTTGGTGGCTTATCAGTGATAAGGCTAAAACCACCTTCAAGAGATGAAGAATTATGAATAGTACTTGAATTTATTGCTTTTCTTTGAGTGATGTATTGATCTGATGAGCTTTGAGACACCGTTTCGTCAAGTTCAACATTGAATGGACATTGCATGTCTTTCTTACCAACGCGTCTGATCAAAGATGTATCTGGTGTCTGTTTCTGTGTTTTATAACTTATACCATTTGAACCAACACTTCTGATTGAATGTGTGTCTGGTGCACGTCTCTGTCTGAATGCTTCGTAACTTGTACCATTTAAACCAA
TARGET=538,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1977
PRIMER_LEFT_PENALTY=0.102129
PRIMER_RIGHT_PENALTY=0.095538
PRIMER_LEFT_SEQUENCE=CACAGTTTGTGGATCGTTTGAT
PRIMER_RIGHT_SEQUENCE=GCACCTTGTTCAACCAACATAA
PRIMER_LEFT=361,22
PRIMER_RIGHT=593,22
PRIMER_LEFT_TM=59.898
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.2062
PRIMER_LEFT_1_PENALTY=0.173816
PRIMER_RIGHT_1_PENALTY=0.032347
PRIMER_LEFT_1_SEQUENCE=ATCACATTGATGTCGTCGATTC
PRIMER_RIGHT_1_SEQUENCE=TGATAAGCCACCAACAAACAAG
PRIMER_LEFT_1=415,22
PRIMER_RIGHT_1=633,22
PRIMER_LEFT_1_TM=59.826
PRIMER_RIGHT_1_TM=60.032
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=0.2062
PRIMER_LEFT_2_PENALTY=0.173816
PRIMER_RIGHT_2_PENALTY=0.032347
PRIMER_LEFT_2_SEQUENCE=ATCACATTGATGTCGTCGATTC
PRIMER_RIGHT_2_SEQUENCE=CTGATAAGCCACCAACAAACAA
PRIMER_LEFT_2=415,22
PRIMER_RIGHT_2=634,22
PRIMER_LEFT_2_TM=59.826
PRIMER_RIGHT_2_TM=60.032
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.2694
PRIMER_LEFT_3_PENALTY=0.173816
PRIMER_RIGHT_3_PENALTY=0.095538
PRIMER_LEFT_3_SEQUENCE=ATCACATTGATGTCGTCGATTC
PRIMER_RIGHT_3_SEQUENCE=GCACCTTGTTCAACCAACATAA
PRIMER_LEFT_3=415,22
PRIMER_RIGHT_3=593,22
PRIMER_LEFT_3_TM=59.826
PRIMER_RIGHT_3_TM=59.904
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=179
PRIMER_PAIR_PENALTY_4=0.2766
PRIMER_LEFT_4_PENALTY=0.102129
PRIMER_RIGHT_4_PENALTY=0.174518
PRIMER_LEFT_4_SEQUENCE=CACAGTTTGTGGATCGTTTGAT
PRIMER_RIGHT_4_SEQUENCE=ACCAAGATCTGACAAAGCACCT
PRIMER_LEFT_4=361,22
PRIMER_RIGHT_4=609,22
PRIMER_LEFT_4_TM=59.898
PRIMER_RIGHT_4_TM=60.175
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_05587 Ps_contig_mira-and-tgicl-ass_franssen_38710
SEQUENCE=AGGTTTTTCTTTANCNAATTAAAAATTCAATCAAAACTTTCTATTGCTGAATACAAATACATTTGAATTACTGATTACAATGGAAATCAGACTAAATACATCACACTAATTTGTTACATTTCAAAACCCTAATTCTATTTTCAGACAATATCAAAACCTAATCAAAATTACAATTAAGCTGAATCAATCAAATTAATAACTTCCTAAAACTACAAATCTAAATAAAACATAAAAATTAAGATTAATCTATATCAACCTAATATTAAAATAAAGATGGGGGAGAGAACCCTAGATAAAAACTTCATTGCCCGCTGTTGTATTCCTCAAACACCACCAAAGCTTTCAGATTAGTACTTAGGGTTTTTTAGTGAAACCCTAACTCTCTGAGCTAAGCTCCATTCAAACCTCGTAAAAACGGTCAAAAGAAACAAGGAGTCGGAAGGAGAAATAGATAGGGATCGAGGAGAGGAATTCAAAAAAGAAGTGAGATTTTTACCTGTTACCGGAGAAGACCTCATCTCCCAAAGTTGGTCCTCATTTTTGTATTTCGATGCTTGAATTATAGTTTTGATGGTTGAATAAGGATCAGAGGGTTGTGATTTAGGGTTTGGGAAATGTTTGATGTGTTTCTGGGTAGATTCAGAGCCAATCTGAGTTATGTTCAGGTCAGGGTTGGAGGAAGAAGATGAAGACTCATCTTCTTCGGGTTATGGTTATTAAGAGGTTGTTAGGGTTTATCTGAACCTAGTTAGGGTTCTTAGGGTAAAGGGCTAGGGTTCGTCAAGGGAACCCTATGGTTCTTGGGTTAGGGTAGGGTTCATAAATTTCTGGGTAGGGTTTGTATTAGGGTTCTTGGGTTTAGGGGTAGGGTTCTTGGGTTTAGGGGTAGGGTTCTTAGGTTTGAAGGGTTCTGGGTAGGTCTTCATCATCCGGATGAACACCAGTTGAAGATCCCGGTTGAACTTCAACCAGAGTTCTAGAGGTTACGATAGTTCTGAGAAGGGAGACAGTGATGGTCGGTTTCTGGGGTGGATGTTCTTCATCTTCATGTTGAACATGAAAAACAAGGTTGAAGGTGGTGGTTCCGGGAATGTTTAGGATGGAAGAAAGGGTGGTCGAGAAAGCAAAGGGAAGAGAGAAGAGAGAGGCTACGAGAGAGAGAAGTTTAGAGAGAGAAAGAAGAAATGAAAGTCYCYGCGTNCT
TARGET=1153,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05588 Ps_contig_mira-and-tgicl-ass_franssen_38711
SEQUENCE=AAGGGTATTGCATTTGAGTGAAAGTTAGTGTACAACATCACAAGGGCAGAGTATTGCATTTGAGTGAAAGTTACAAGTGTACAAGAACAATGGCAACTCCAGTGCAAGTGTACGGACCAGCCTTATCGACGGCGGTTTCCAGAGTGTTGACATGTCTGAACGAGAAAGACATTCCATTCCAACTTGTTCCTGTCAACATGTCCAAAGAAGAACACCGAAGCCCTGATTTCCTCAAAATCCATCCATTTGCCCAAGTACCTGCATTTCAGGATGACCACATTTCCCTCTTCGAGTAACCAATTTATTAACTTATTGCTTCATACTTAATTATTATTATTACTATGATTTGTTTTATTAATTCAGAGTCTAGAGCCATATGCCGGTATGTTTGTGAGAAACACATAGAGAAAGGAAACAGGGAACTGTACGGTACAAACCCACTGGCAAAGGCATCTATAGATCAATGGTTGGAGGCTGAAGGACAGAACTTCAACCCACCCTCATCAACTCTGGTCTTTCAGCTTGCATTTGCTCCTCGAATGAAGGTTAAGCAAGACGAAGGAGCAATCAAACAGAGTAAAGAAAAGCTCAAGAAAGTGCTTGATGTGTACGATAGGAAGCTTGGGGAAACTCGTTACTTGGCCGGTGAGGAATTCACACTCGCAGATCTTTCGCATCTTCCCAATATTCATTACTTGGTGAGTGCTGATGATGATACGGCTTCTCTCTTCACTTCTGAGAGAGAGAACGTGAGGAGGTGGTGGACGGAGATATCCGCGCGCGAGTCATGGAAAAAGGTTGTTGATTTGTACCCAAAACCTTGACTTCAGATCAACAGATTAGCTTTCTTTACTTTATATATTTGCCATTTCCAATGTGGTTGTTGACACACCTCCTTTATTTGTATCTTGAAAATCTACTATTCTGGTCATATCTAGCATGTTAATTTCTCAAACTANCAAAGCTAAATTTTTTAC
TARGET=327,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1101
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.107763
PRIMER_LEFT_SEQUENCE=ACCACATTTCCCTCTTCGAGTA
PRIMER_RIGHT_SEQUENCE=TCCTTCGTCTTGCTTAACCTTC
PRIMER_LEFT=273,22
PRIMER_RIGHT=562,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=290
PRIMER_PAIR_PENALTY_1=0.1383
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.135999
PRIMER_LEFT_1_SEQUENCE=ACCACATTTCCCTCTTCGAGTA
PRIMER_RIGHT_1_SEQUENCE=TACCGGCATATGGCTCTAGACT
PRIMER_LEFT_1=273,22
PRIMER_RIGHT_1=384,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.136
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=112
PRIMER_PAIR_PENALTY_2=0.1443
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.135999
PRIMER_LEFT_2_SEQUENCE=CATTCCAACTTGTTCCTGTCAA
PRIMER_RIGHT_2_SEQUENCE=TACCGGCATATGGCTCTAGACT
PRIMER_LEFT_2=174,22
PRIMER_RIGHT_2=384,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.136
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.1703
PRIMER_LEFT_3_PENALTY=0.060196
PRIMER_RIGHT_3_PENALTY=0.110131
PRIMER_LEFT_3_SEQUENCE=ATTTCAGGATGACCACATTTCC
PRIMER_RIGHT_3_SEQUENCE=GAAAGACCAGAGTTGATGAGGG
PRIMER_LEFT_3=262,22
PRIMER_RIGHT_3=518,22
PRIMER_LEFT_3_TM=60.060
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.1962
PRIMER_LEFT_4_PENALTY=0.060196
PRIMER_RIGHT_4_PENALTY=0.135999
PRIMER_LEFT_4_SEQUENCE=ATTTCAGGATGACCACATTTCC
PRIMER_RIGHT_4_SEQUENCE=TACCGGCATATGGCTCTAGACT
PRIMER_LEFT_4=262,22
PRIMER_RIGHT_4=384,22
PRIMER_LEFT_4_TM=60.060
PRIMER_RIGHT_4_TM=60.136
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_05589 Ps_contig_mira-and-tgicl-ass_franssen_38711
SEQUENCE=AAGGGTATTGCATTTGAGTGAAAGTTAGTGTACAACATCACAAGGGCAGAGTATTGCATTTGAGTGAAAGTTACAAGTGTACAAGAACAATGGCAACTCCAGTGCAAGTGTACGGACCAGCCTTATCGACGGCGGTTTCCAGAGTGTTGACATGTCTGAACGAGAAAGACATTCCATTCCAACTTGTTCCTGTCAACATGTCCAAAGAAGAACACCGAAGCCCTGATTTCCTCAAAATCCATCCATTTGCCCAAGTACCTGCATTTCAGGATGACCACATTTCCCTCTTCGAGTAACCAATTTATTAACTTATTGCTTCATACTTAATTATTATTATTACTATGATTTGTTTTATTAATTCAGAGTCTAGAGCCATATGCCGGTATGTTTGTGAGAAACACATAGAGAAAGGAAACAGGGAACTGTACGGTACAAACCCACTGGCAAAGGCATCTATAGATCAATGGTTGGAGGCTGAAGGACAGAACTTCAACCCACCCTCATCAACTCTGGTCTTTCAGCTTGCATTTGCTCCTCGAATGAAGGTTAAGCAAGACGAAGGAGCAATCAAACAGAGTAAAGAAAAGCTCAAGAAAGTGCTTGATGTGTACGATAGGAAGCTTGGGGAAACTCGTTACTTGGCCGGTGAGGAATTCACACTCGCAGATCTTTCGCATCTTCCCAATATTCATTACTTGGTGAGTGCTGATGATGATACGGCTTCTCTCTTCACTTCTGAGAGAGAGAACGTGAGGAGGTGGTGGACGGAGATATCCGCGCGCGAGTCATGGAAAAAGGTTGTTGATTTGTACCCAAAACCTTGACTTCAGATCAACAGATTAGCTTTCTTTACTTTATATATTTGCCATTTCCAATGTGGTTGTTGACACACCTCCTTTATTTGTATCTTGAAAATCTACTATTCTGGTCATATCTAGCATGTTAATTTCTCAAACTANCAAAGCTAAATTTTTTAC
TARGET=738,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0829
PRIMER_LEFT_PENALTY=0.059316
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=GCTCAAGAAAGTGCTTGATGTG
PRIMER_RIGHT_SEQUENCE=GATCTGAAGTCAAGGTTTTGGG
PRIMER_LEFT=586,22
PRIMER_RIGHT=832,22
PRIMER_LEFT_TM=60.059
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=247
PRIMER_PAIR_PENALTY_1=0.1313
PRIMER_LEFT_1_PENALTY=0.107763
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=GAAGGTTAAGCAAGACGAAGGA
PRIMER_RIGHT_1_SEQUENCE=GATCTGAAGTCAAGGTTTTGGG
PRIMER_LEFT_1=541,22
PRIMER_RIGHT_1=832,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.2118
PRIMER_LEFT_2_PENALTY=0.188254
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=ACGGCTTCTCTCTTCACTTCTG
PRIMER_RIGHT_2_SEQUENCE=GATCTGAAGTCAAGGTTTTGGG
PRIMER_LEFT_2=716,22
PRIMER_RIGHT_2=832,22
PRIMER_LEFT_2_TM=60.188
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=0.2728
PRIMER_LEFT_3_PENALTY=0.249232
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=CCTCGAATGAAGGTTAAGCAAG
PRIMER_RIGHT_3_SEQUENCE=GATCTGAAGTCAAGGTTTTGGG
PRIMER_LEFT_3=533,22
PRIMER_RIGHT_3=832,22
PRIMER_LEFT_3_TM=60.249
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.3959
PRIMER_LEFT_4_PENALTY=0.188254
PRIMER_RIGHT_4_PENALTY=0.207669
PRIMER_LEFT_4_SEQUENCE=ACGGCTTCTCTCTTCACTTCTG
PRIMER_RIGHT_4_SEQUENCE=GAGGTGTGTCAACAACCACATT
PRIMER_LEFT_4=716,22
PRIMER_RIGHT_4=894,22
PRIMER_LEFT_4_TM=60.188
PRIMER_RIGHT_4_TM=59.792
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_05590 Ps_contig_mira-and-tgicl-ass_franssen_38715
SEQUENCE=CGGGGTTTTGGTTTTTGTTTTTTATTTTCTGGGGNATGCCACATTGAAACTATTTCAACACTCTTGTTTTCTTCTCTCTCTCCAAAAAAAAATCATTAGTTTCCCATAATGTCTCTTACCATCAAACTACAATTACTTTCTCACCATAACATCAACCACAACAACAACAATCATGCAAACAAATTATTAAAAAACAACACTTTATGTTACAATACTAAGTTTCTTCCAAAACCCATTTTCCATAACAATCCATTCATCTTTACAAAGTGTAAAAAAGTGAAAAGAAGAATGCAAGAAAGTTTCATGATCAAAAGTTCTTTAATTGAGCCAGATGGGGGTGTGTTAGTAGATCTTGTGGTGCCTGAAAATCAAAGAGATTCTAAGGTGCTTGAAGCTAAGTCACTTCCTAATGTGAAACTAACAAAGGTTGATTTTGAATGGGTGCATGTGATTTGTGAAGGTTGGGCTAGTCCTTTGAAAGGGTTCATGAGGGAAAATGAGTATCTTCAAAGTTTGCTA
TARGET=73,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4834
PRIMER_LEFT_PENALTY=0.703260
PRIMER_RIGHT_PENALTY=0.780133
PRIMER_LEFT_SEQUENCE=GGGNATGCCACATTGAAACTAT
PRIMER_RIGHT_SEQUENCE=TGTTGTGGTTGATGTTATGGTG
PRIMER_LEFT=31,22
PRIMER_RIGHT=162,22
PRIMER_LEFT_TM=60.703
PRIMER_RIGHT_TM=59.220
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.50
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=1.8033
PRIMER_LEFT_1_PENALTY=0.703260
PRIMER_RIGHT_1_PENALTY=1.100059
PRIMER_LEFT_1_SEQUENCE=GGGNATGCCACATTGAAACTAT
PRIMER_RIGHT_1_SEQUENCE=TGTGGTTGATGTTATGGTGAGA
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=159,22
PRIMER_LEFT_1_TM=60.703
PRIMER_RIGHT_1_TM=58.900
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.50
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.3000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=2.1893
PRIMER_LEFT_2_PENALTY=1.469712
PRIMER_RIGHT_2_PENALTY=0.719637
PRIMER_LEFT_2_SEQUENCE=GGGNATGCCACATTGAAACTA
PRIMER_RIGHT_2_SEQUENCE=GCATGATTGTTGTTGTTGTGGT
PRIMER_LEFT_2=31,21
PRIMER_RIGHT_2=175,22
PRIMER_LEFT_2_TM=60.470
PRIMER_RIGHT_2_TM=60.720
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.50
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=2.2498
PRIMER_LEFT_3_PENALTY=1.469712
PRIMER_RIGHT_3_PENALTY=0.780133
PRIMER_LEFT_3_SEQUENCE=GGGNATGCCACATTGAAACTA
PRIMER_RIGHT_3_SEQUENCE=TGTTGTGGTTGATGTTATGGTG
PRIMER_LEFT_3=31,21
PRIMER_RIGHT_3=162,22
PRIMER_LEFT_3_TM=60.470
PRIMER_RIGHT_3_TM=59.220
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.50
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=2.2979
PRIMER_LEFT_4_PENALTY=0.703260
PRIMER_RIGHT_4_PENALTY=1.594644
PRIMER_LEFT_4_SEQUENCE=GGGNATGCCACATTGAAACTAT
PRIMER_RIGHT_4_SEQUENCE=TTGTGGTTGATGTTATGGTGAG
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=160,22
PRIMER_LEFT_4_TM=60.703
PRIMER_RIGHT_4_TM=58.405
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.50
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_05591 Ps_contig_mira-and-tgicl-ass_franssen_38715
SEQUENCE=CGGGGTTTTGGTTTTTGTTTTTTATTTTCTGGGGNATGCCACATTGAAACTATTTCAACACTCTTGTTTTCTTCTCTCTCTCCAAAAAAAAATCATTAGTTTCCCATAATGTCTCTTACCATCAAACTACAATTACTTTCTCACCATAACATCAACCACAACAACAACAATCATGCAAACAAATTATTAAAAAACAACACTTTATGTTACAATACTAAGTTTCTTCCAAAACCCATTTTCCATAACAATCCATTCATCTTTACAAAGTGTAAAAAAGTGAAAAGAAGAATGCAAGAAAGTTTCATGATCAAAAGTTCTTTAATTGAGCCAGATGGGGGTGTGTTAGTAGATCTTGTGGTGCCTGAAAATCAAAGAGATTCTAAGGTGCTTGAAGCTAAGTCACTTCCTAATGTGAAACTAACAAAGGTTGATTTTGAATGGGTGCATGTGATTTGTGAAGGTTGGGCTAGTCCTTTGAAAGGGTTCATGAGGGAAAATGAGTATCTTCAAAGTTTGCTA
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9309
PRIMER_LEFT_PENALTY=0.703260
PRIMER_RIGHT_PENALTY=2.227592
PRIMER_LEFT_SEQUENCE=GGGNATGCCACATTGAAACTAT
PRIMER_RIGHT_SEQUENCE=TGGAAAATGGGTTTTGGAAG
PRIMER_LEFT=31,22
PRIMER_RIGHT=241,20
PRIMER_LEFT_TM=60.703
PRIMER_RIGHT_TM=59.772
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=5.50
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=3.4514
PRIMER_LEFT_1_PENALTY=3.019823
PRIMER_RIGHT_1_PENALTY=0.431597
PRIMER_LEFT_1_SEQUENCE=CCCATAATGTCTCTTACCATCAAAC
PRIMER_RIGHT_1_SEQUENCE=CCCATCTGGCTCAATTAAAGAA
PRIMER_LEFT_1=102,25
PRIMER_RIGHT_1=335,22
PRIMER_LEFT_1_TM=60.020
PRIMER_RIGHT_1_TM=60.432
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=3.6973
PRIMER_LEFT_2_PENALTY=1.469712
PRIMER_RIGHT_2_PENALTY=2.227592
PRIMER_LEFT_2_SEQUENCE=GGGNATGCCACATTGAAACTA
PRIMER_RIGHT_2_SEQUENCE=TGGAAAATGGGTTTTGGAAG
PRIMER_LEFT_2=31,21
PRIMER_RIGHT_2=241,20
PRIMER_LEFT_2_TM=60.470
PRIMER_RIGHT_2_TM=59.772
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.50
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=3.7164
PRIMER_LEFT_3_PENALTY=3.284822
PRIMER_RIGHT_3_PENALTY=0.431597
PRIMER_LEFT_3_SEQUENCE=CTCTTGTTTTCTTCTCTCTCTCCAA
PRIMER_RIGHT_3_SEQUENCE=CCCATCTGGCTCAATTAAAGAA
PRIMER_LEFT_3=60,25
PRIMER_RIGHT_3=335,22
PRIMER_LEFT_3_TM=59.715
PRIMER_RIGHT_3_TM=60.432
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=3.7775
PRIMER_LEFT_4_PENALTY=3.019823
PRIMER_RIGHT_4_PENALTY=0.757660
PRIMER_LEFT_4_SEQUENCE=CCCATAATGTCTCTTACCATCAAAC
PRIMER_RIGHT_4_SEQUENCE=TTTCAGGCACCACAAGATCTAC
PRIMER_LEFT_4=102,25
PRIMER_RIGHT_4=366,22
PRIMER_LEFT_4_TM=60.020
PRIMER_RIGHT_4_TM=59.242
PRIMER_LEFT_4_GC_PERCENT=40.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=265
=
PRIMER_SEQUENCE_ID=SSR_05592 Ps_contig_mira-and-tgicl-ass_franssen_38743
SEQUENCE=AAATAACCAGAAGCATCAACTCAACAGATCCTCGTCACTCTTGGAAAAACAACATTCGAAAAACGATCCGATGAATCAAGTTGTGATCACGTAAGGAAAAATCCGAGGTAACCCATTCTGTCTCTGACAAATGAAGAAAAAAAGTACAAACTAGGAAAATTGTCGGAGAATGCTTGAGATCTAAACGGAATTCCGAAGTGAAACACAAAAACCTCACAAGTTTTTTTCTCTGTTCTTCAATAATAATAATGTGAAATTTTATTTTTTAATATTTTTAAAAAGAAATAATTGTTATGTGATTGTGAGATGAAAGAAAACAATAGAATAGATAGAATAGTAGATAGAAGAGTTTAGGACACAAAAACCCACCAATCATAAACCCG
TARGET=239,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4229
PRIMER_LEFT_PENALTY=0.075753
PRIMER_RIGHT_PENALTY=0.347129
PRIMER_LEFT_SEQUENCE=AAAATCCGAGGTAACCCATTCT
PRIMER_RIGHT_SEQUENCE=GGTGGGTTTTTGTGTCCTAAAC
PRIMER_LEFT=97,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=60.076
PRIMER_RIGHT_TM=59.653
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=273
PRIMER_PAIR_PENALTY_1=0.4498
PRIMER_LEFT_1_PENALTY=0.102638
PRIMER_RIGHT_1_PENALTY=0.347129
PRIMER_LEFT_1_SEQUENCE=ATTGTCGGAGAATGCTTGAGAT
PRIMER_RIGHT_1_SEQUENCE=GGTGGGTTTTTGTGTCCTAAAC
PRIMER_LEFT_1=158,22
PRIMER_RIGHT_1=369,22
PRIMER_LEFT_1_TM=60.103
PRIMER_RIGHT_1_TM=59.653
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.5700
PRIMER_LEFT_2_PENALTY=0.075753
PRIMER_RIGHT_2_PENALTY=0.494198
PRIMER_LEFT_2_SEQUENCE=AAAATCCGAGGTAACCCATTCT
PRIMER_RIGHT_2_SEQUENCE=ATTGGTGGGTTTTTGTGTCCTA
PRIMER_LEFT_2=97,22
PRIMER_RIGHT_2=372,22
PRIMER_LEFT_2_TM=60.076
PRIMER_RIGHT_2_TM=60.494
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.5968
PRIMER_LEFT_3_PENALTY=0.102638
PRIMER_RIGHT_3_PENALTY=0.494198
PRIMER_LEFT_3_SEQUENCE=ATTGTCGGAGAATGCTTGAGAT
PRIMER_RIGHT_3_SEQUENCE=ATTGGTGGGTTTTTGTGTCCTA
PRIMER_LEFT_3=158,22
PRIMER_RIGHT_3=372,22
PRIMER_LEFT_3_TM=60.103
PRIMER_RIGHT_3_TM=60.494
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=0.6593
PRIMER_LEFT_4_PENALTY=0.312177
PRIMER_RIGHT_4_PENALTY=0.347129
PRIMER_LEFT_4_SEQUENCE=GATCACGTAAGGAAAAATCCGA
PRIMER_RIGHT_4_SEQUENCE=GGTGGGTTTTTGTGTCCTAAAC
PRIMER_LEFT_4=84,22
PRIMER_RIGHT_4=369,22
PRIMER_LEFT_4_TM=60.312
PRIMER_RIGHT_4_TM=59.653
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_05593 Ps_contig_mira-and-tgicl-ass_franssen_38759
SEQUENCE=AATTTCATTCTAAAATCGAGCATGTTTCCATTCAATGAAGATCATTCCCATTCCAAATAAGTGTACATTCATTCATTCAATTTGCATTTATGACATTTATACAAGAAATTCACAAAAAAAAATGAAAATTTGGCCAAAAGTTGATTTTTGGTCAGCTATTGACTTTTGGGTCAACTGTTGACTTTTTTGGTCAACCAACATACTACTTCATAACCTAAGCCCTAGC
TARGET=66,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7644
PRIMER_LEFT_PENALTY=0.510901
PRIMER_RIGHT_PENALTY=0.253482
PRIMER_LEFT_SEQUENCE=CAATGAAGATCATTCCCATTCC
PRIMER_RIGHT_SEQUENCE=TGACCCAAAAGTCAATAGCTGA
PRIMER_LEFT=32,22
PRIMER_RIGHT=172,22
PRIMER_LEFT_TM=60.511
PRIMER_RIGHT_TM=59.747
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.7644
PRIMER_LEFT_1_PENALTY=0.510901
PRIMER_RIGHT_1_PENALTY=0.253482
PRIMER_LEFT_1_SEQUENCE=CCATTCAATGAAGATCATTCCC
PRIMER_RIGHT_1_SEQUENCE=TGACCCAAAAGTCAATAGCTGA
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=172,22
PRIMER_LEFT_1_TM=60.511
PRIMER_RIGHT_1_TM=59.747
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.9848
PRIMER_LEFT_2_PENALTY=0.510901
PRIMER_RIGHT_2_PENALTY=0.473868
PRIMER_LEFT_2_SEQUENCE=CAATGAAGATCATTCCCATTCC
PRIMER_RIGHT_2_SEQUENCE=ACCCAAAAGTCAATAGCTGACC
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=170,22
PRIMER_LEFT_2_TM=60.511
PRIMER_RIGHT_2_TM=59.526
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=0.9848
PRIMER_LEFT_3_PENALTY=0.510901
PRIMER_RIGHT_3_PENALTY=0.473868
PRIMER_LEFT_3_SEQUENCE=CCATTCAATGAAGATCATTCCC
PRIMER_RIGHT_3_SEQUENCE=ACCCAAAAGTCAATAGCTGACC
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=170,22
PRIMER_LEFT_3_TM=60.511
PRIMER_RIGHT_3_TM=59.526
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=1.2440
PRIMER_LEFT_4_PENALTY=0.510901
PRIMER_RIGHT_4_PENALTY=0.733091
PRIMER_LEFT_4_SEQUENCE=CAATGAAGATCATTCCCATTCC
PRIMER_RIGHT_4_SEQUENCE=CCAAAAGTCAATAGCTGACCAA
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=168,22
PRIMER_LEFT_4_TM=60.511
PRIMER_RIGHT_4_TM=59.267
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_05594 Ps_contig_mira-and-tgicl-ass_franssen_38765
SEQUENCE=ATGATATTTAAAAATATCCAAAATAAATAAATAAAATTAAAACAAAAGATGATCCACTTCGAACTGTAGTTGACATCTGGTGTCTAGTGGCCACACGAGCTTTACCACTAACCCTTATGGCTATGTCCATATGCAATTCATCACAATGCTTCGTTCTCTCCACTCTCTCTTCTCCACGGAATATTCTGTGACCGAGATGGTTGTTTTGAGTCCTTTTTGGATTTTTTTAGGGTTTTGCTTCCACATGCCCTATACTACATGGACGAAAAACTAAATTCAAAGGCGGAAAAGGGAGACCGG
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05595 Ps_contig_mira-and-tgicl-ass_franssen_38784
SEQUENCE=CCTTTTTTAATTTGTCTCTGACCTCATCCCAAACATACCACCACTATAATAGCTACACCCCCTTTTCGATGTATTAAACTATAATCAAAAGGTTATTGTATATTTTTTCGTCTTAACTCTCCGATTTTTACAGAGACGRCATTCAATTATAATTCCGTGTGACGAATATCATCATCATCTTGAGAAGTAATAGATTGAGATTCATGAACTTGAGAGGTTTTGTCATTGGAATTAAGGCAATCAGCAGAAGGATGAACTACACCATATCCAAATCCTTGAGCTGAATGGCAATATGTTTCAACTGTGCCATCTTGTTTCTCCAAATCAACATTGCTAAGGATTAATTTACTGCATGGAACACTGTCACTACAATCAAATTTAATGGCCTTAGCACTATTTGTAGTCCCGCTTATGTTTTGGTA
TARGET=168,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05596 Ps_contig_mira-and-tgicl-ass_franssen_38790
SEQUENCE=AACAGCGCCCAAGCTATGTTTGAGATTCCTTGAGCCGAGCAATCAGGTAAGGATGTCATTGCAATGCCAACAAGCTTAGACATCTCTTTTTGTCGAGCAAACGCGAGTCTTCGAGTTTTCATCATAGAAACTTTCTCCATATTCTTAGCAATACGATGAAGAGCAGTAGCAATATTCAAAGGCGAGAGAGGCGACGGGCTAAGTCCTTTCGCAACTGCAACAATTGTTTCGTATGTAACATCCAACACATCCTCTGCAGTCTGTGCTTCAATGATCGCTTCGTTCAAGTTCATTTCTTTTCTATGGTCAGATATCCCAGAAAATTGAGAGGGTCTATTGTAAAATGTGTCAATCGTTTTCGCCCTATTTTCTGCAAACTTTTCATCCTCGACCAAACCTACTTTCCTTTTCAAATCAATTTTTTCGATTTTCTTATCTGTTTTCTCCTTGCTTACCAACTTCTTCTTAGCCTTTTTCTTCTTCTTCTTAACAGTAATCATATCATCCATTGCATAATCCATCTGCTTCGCTTTAATTGTTTTCAGTGCCATCTCTCTTGCCCTCACACACCAACCCATTTCATTCGTATCTTCAAGC
TARGET=475,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1768
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.174518
PRIMER_LEFT_SEQUENCE=TCATCCTCGACCAAACCTACTT
PRIMER_RIGHT_SEQUENCE=CAGATGGATTATGCAATGGATG
PRIMER_LEFT=381,22
PRIMER_RIGHT=523,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.175
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=143
PRIMER_PAIR_PENALTY_1=0.4274
PRIMER_LEFT_1_PENALTY=0.352395
PRIMER_RIGHT_1_PENALTY=0.075027
PRIMER_LEFT_1_SEQUENCE=TTTCATCCTCGACCAAACCTAC
PRIMER_RIGHT_1_SEQUENCE=AGATACGAATGAAATGGGTTGG
PRIMER_LEFT_1=379,22
PRIMER_RIGHT_1=590,22
PRIMER_LEFT_1_TM=60.352
PRIMER_RIGHT_1_TM=60.075
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.4279
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.425548
PRIMER_LEFT_2_SEQUENCE=TCATCCTCGACCAAACCTACTT
PRIMER_RIGHT_2_SEQUENCE=GAAGCAGATGGATTATGCAATG
PRIMER_LEFT_2=381,22
PRIMER_RIGHT_2=527,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=59.574
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=0.4279
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.425548
PRIMER_LEFT_3_SEQUENCE=TTCATCCTCGACCAAACCTACT
PRIMER_RIGHT_3_SEQUENCE=GAAGCAGATGGATTATGCAATG
PRIMER_LEFT_3=380,22
PRIMER_RIGHT_3=527,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=59.574
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=0.4605
PRIMER_LEFT_4_PENALTY=0.411917
PRIMER_RIGHT_4_PENALTY=0.048570
PRIMER_LEFT_4_SEQUENCE=CCCAGAAAATTGAGAGGGTCTA
PRIMER_RIGHT_4_SEQUENCE=GATACGAATGAAATGGGTTGGT
PRIMER_LEFT_4=314,22
PRIMER_RIGHT_4=589,22
PRIMER_LEFT_4_TM=59.588
PRIMER_RIGHT_4_TM=59.951
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=276
=
PRIMER_SEQUENCE_ID=SSR_05597 Ps_contig_mira-and-tgicl-ass_franssen_38800
SEQUENCE=AATNAAATTNAATTCAATCCAATCCAATCCATCTTCTAATCTTTAACCTAAACGATGAAATTCGGAAAGAGTCTCAACAACCAGATCGAGAAAACCGTTCCTCAATGGCGTGACAAATTTCTCTCTTACAAGCTACTCAAGAAGAAATTGAAGCTTGTTCAACCAACCTCCGATGAACGCCCGAACAAAAGAGCTAGGATCGACGACGGAGAAATGTCAAATGAAGAAACCGATTTCCGGAATTCCCTCGAAGATGAACTCCACAAATTCAATTCTTTCTTCGTTGAGAAAGAGGAAGAG
TARGET=15,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05598 Ps_contig_mira-and-tgicl-ass_franssen_38812
SEQUENCE=AAAACTACAAATTATCGCCCCAAGTTCACACGATGGCTCATTAAATTCAAACTATCACATCTTAGGAAACAAAATACATACATTTGGATACAATCAATTTCCAAATGGAGTGGCTAAAAATCCAATCTCTAAACTAGGAGCTACACTATCAAATGCCTAAAACTATCAACAAATATCCAAAAGGTTTTCCAAATATCCATAGAAACAGAAAATGAAAATGTGCTAAAGAAATTCAATCACCCTTCCTAAGTACCTCTCCTTGGTTATATAGATCTAATTCCAATTGTTAATACAATTCAACTTCTGACATCATCTCTAAAGTTGATGATGTCAAGACACAAACCAATGCTCTGGTCTAGTGTTATGTGGTAAGAAATTTCCATAGCTTCTTCCCTACTGAAGTTTCACCAGGTTGTTGATAATCTTCATCAATCTCGTTCTCCTCGTTCTCCTCCTCCTCATCCTCAACTCCTCGGGATTCACTCCTGTACTCGGCATCATGATCCTCCGCGTCATCCGCTGTTCCGTTCACCTCCTCACTCAAAGCCATGTTGCTTGCAAAAGTCCTGGCGGTATTGTCGAAATCATCATGTGTCTCCACTACTTTCTGAGACTCATCAAGATCTATGCTGCCACCAATTTCGTTGGTAATGCCGACCGAGTGGGAATGAGAAGCTTCTGCAGCTTTTGCAGGACCAACCTCTCCCTCGCCTTCCTTGCCGCCACCAGACACGTCTCTAACAGATGAAGTTGTCGCTCGAGTTTTGGGTCGCCTCAAATTATATCTTGTTTCTCCAGCAGTTTGTACAGGAGGAGGAGCAGCCTTCTGTCGAGTCCTTCTGCGCTGTCCTGGTATATCAGAATGTCCTTCACTCGCACTGGCAGTAACTTGAGATGTTGTTTGAACCCGGTTTCGCTTCCTCAAATTTGCCGTTCTTTTTTCGGCTGGTTTTTGACTTTCAGAATCCATGTTGGCAGAATCCTCAGCATTTCCATTTGGAAACCCAGTCTCACGGTCATCCACGCACTCCCCAGGTACTGCC
TARGET=449,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2272
PRIMER_LEFT_PENALTY=0.095523
PRIMER_RIGHT_PENALTY=0.131699
PRIMER_LEFT_SEQUENCE=ATGTCAAGACACAAACCAATGC
PRIMER_RIGHT_SEQUENCE=GAGTACAGGAGTGAATCCCGAG
PRIMER_LEFT=327,22
PRIMER_RIGHT=492,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=60.132
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.3049
PRIMER_LEFT_1_PENALTY=0.095523
PRIMER_RIGHT_1_PENALTY=0.209343
PRIMER_LEFT_1_SEQUENCE=ATGTCAAGACACAAACCAATGC
PRIMER_RIGHT_1_SEQUENCE=CATGATGATTTCGACAATACCG
PRIMER_LEFT_1=327,22
PRIMER_RIGHT_1=591,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=60.209
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=8.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.3516
PRIMER_LEFT_2_PENALTY=0.219884
PRIMER_RIGHT_2_PENALTY=0.131699
PRIMER_LEFT_2_SEQUENCE=TCAAGACACAAACCAATGCTCT
PRIMER_RIGHT_2_SEQUENCE=GAGTACAGGAGTGAATCCCGAG
PRIMER_LEFT_2=330,22
PRIMER_RIGHT_2=492,22
PRIMER_LEFT_2_TM=59.780
PRIMER_RIGHT_2_TM=60.132
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=0.4292
PRIMER_LEFT_3_PENALTY=0.219884
PRIMER_RIGHT_3_PENALTY=0.209343
PRIMER_LEFT_3_SEQUENCE=TCAAGACACAAACCAATGCTCT
PRIMER_RIGHT_3_SEQUENCE=CATGATGATTTCGACAATACCG
PRIMER_LEFT_3=330,22
PRIMER_RIGHT_3=591,22
PRIMER_LEFT_3_TM=59.780
PRIMER_RIGHT_3_TM=60.209
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.4793
PRIMER_LEFT_4_PENALTY=0.095523
PRIMER_RIGHT_4_PENALTY=0.383798
PRIMER_LEFT_4_SEQUENCE=ATGTCAAGACACAAACCAATGC
PRIMER_RIGHT_4_SEQUENCE=GTGGAGACACATGATGATTTCG
PRIMER_LEFT_4=327,22
PRIMER_RIGHT_4=600,22
PRIMER_LEFT_4_TM=59.904
PRIMER_RIGHT_4_TM=60.384
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_05599 Ps_contig_mira-and-tgicl-ass_franssen_38832
SEQUENCE=GATAACAAGACAACAAACTATTTATTTATTATTCACAGCCTCCATCTCCACATTATTCATACAAAGAATAAYAACAATTGATGCCAATACCTATCGAATGGCATCACCACCTACCAAAACTAAACTATATTAAAAATGACTAATAAACAACAATGCATAATTGATATGGAACCATGTGTTGCCAACCCCATTTTTCTTGTTCATCTACGAAAAAGACACAAAACAAAAAGCAACTCTCTTTGGCTCTTTCAATATTTTATTTCTTTTTCAGAACCTAAACAATACAATCATGCACCACAAACACACACCAACCACACATTAACATCAAGCTACAATATCATAACGAATGTGTTTTAGTATCACCGGAATAACAACATCCGGCGAGCTTAACTGCGGTAAATGACCCTCCGTCGACATCACCTCCACGATTGACTCGGTGCCGACGTGTTGGTGGAGATACTCCGCCACTACGACGGGAACAGCCAAGTCCTTCATACTTTGTATAATGTGACATGGGACACTGACGAGACATAATATTTGTCTCATGTCACTTTTGTAAAATTGTTTGTAACACGCTTAGGGCTATGTCTGGTCTCATGTTGAACAAAGTTCGACTGAATTCTTGAACTGCCACTGACTCCATGTCCCCTCCAATCGCCATTGGAGCAAAGCCTGAACACCATGCTTTGTAATTTGATGCCATAGCATTGAATAATTGATCTAAATCTTCTTGTTCAAATCCTCCAAAGTAATTACTGTCGTTCAAATACCTTGGAGAAGCAGATATCATGATGATTTTGGCAAACAAATCAGGACGAGAAATCGATGCAACAGTTCCAATCATAGCAGAAACCGAATGACCAACAAAGATACAAGAATCAACTCTAAGTTCTTGTAATATTGCGAGTAAATCATAAGCATAGCCTTCTAACGTTGAGTAACGTTCGAAATCGAAATAATCTGGATTAGTTGTACCAGCTCCCATGTTGTCATAAAGTATGACACGGAATTCTTCTAGGAGATGAGGTACGAGGTGTTTCCATACAGATTGATCTGTGCCAAATCCGTGAGCTAGAACTATGAACCGTGAACCTGTTCCTAAAACTTTCACGTTGTGTGCTTCTTCCACTATTCCCATTA
TARGET=19,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05600 Ps_contig_mira-and-tgicl-ass_franssen_38843
SEQUENCE=CCAACACAATTGGTAATTAAAAAACTAATACAACAACAACAACCAACACAAGTGGACCTTCAACTCCTCTATTAACTTCACCACTATGTGTCGAAAACATACACTGGACATCTTCATCATTTTCTATACGATCCAGGTAATACATGTACGTACCATCAGGGCTTGCATCAGTCAACGTTATGTATGGACAACGATACTCTACCTTCCTCACCTTCATACGACGCCCGCCCAACTGTCGGAAGCCAGTGTGGATGGTTGAAATCAGTGTTCTGAAATTCCATTGCGGATCAAGGCAAACACTCATTTTTTCACCGTGTCCATAAAATACAAGACCACAAACAGACATTCCAAAAACATCAAGGAACCATGACTCCCTCACCCAGAGCCAACCCATACCATTTTTGTGGTCCTGTTAAGGCGACGCCGATGT
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05601 Ps_contig_mira-and-tgicl-ass_franssen_38851
SEQUENCE=TAAAACCTACTCCACGTCACTCTTCCCTCCACAACTGATCTAACCCCAACTTTGGGTCGAAATACCACCACATTTCTTCTTCTTCATCTTTACGATGACGAGTCCCGGGGGACGGGTACTGTGAAGAAGGTGCTCTCCCTGTGAGAACTTCCAACAGCAACACTCCAAAACTGTAAACATCAGCTTGCTGAGACAGTCTCTTTTGTTCTGTTTGTTCAGGTGCTCTGTACCCTCCCAGTCTTGCAATTGCGTGAACAGGGTTCAACAGCAGTGATAGTCCAAAATCAGAAATGCAAGCAACACCGTTCTTGTCAAGTAGTACATTGGATGATTTCACATTCCCGTGAGGCACTTTTGCTGCACTATACTCTGTATGAATCCTAGCAAGCCCTCTTGCCGCTCCTAACACCAAGCTTATTCTCGTTGTCCAATCCAACGGAATCCTCCCCGGCCCACGGTTCCCATGAAGAAGAGCATGCAAACTTCCATTGGAGAGATAATCATAGACAAGAAGTTTTTCTTCCTTGGCATAATAATAAGCTCTTAGTTTAACAATATTAGGATGTTTAAGCTTCCCAATCACATCCATATACTGCTCAAACTCATGCCTAGCACATGGATTTGCATCTTTCAACCTCTTAACAGCAACTGTACTACCATCATCAAGCACTGCCCTATAAACAGTTCCTAAACTACCCTTCCCAAGCA
TARGET=74,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2460
PRIMER_LEFT_PENALTY=0.199246
PRIMER_RIGHT_PENALTY=0.046801
PRIMER_LEFT_SEQUENCE=CTACTCCACGTCACTCTTCCCT
PRIMER_RIGHT_SEQUENCE=ACAGAGCACCTGAACAAACAGA
PRIMER_LEFT=6,22
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=59.801
PRIMER_RIGHT_TM=59.953
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.3075
PRIMER_LEFT_1_PENALTY=0.260719
PRIMER_RIGHT_1_PENALTY=0.046801
PRIMER_LEFT_1_SEQUENCE=CTCTTCCCTCCACAACTGATCT
PRIMER_RIGHT_1_SEQUENCE=ACAGAGCACCTGAACAAACAGA
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=227,22
PRIMER_LEFT_1_TM=59.739
PRIMER_RIGHT_1_TM=59.953
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.3738
PRIMER_LEFT_2_PENALTY=0.199246
PRIMER_RIGHT_2_PENALTY=0.174518
PRIMER_LEFT_2_SEQUENCE=CTACTCCACGTCACTCTTCCCT
PRIMER_RIGHT_2_SEQUENCE=GAGCACCTTCTTCACAGTACCC
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=134,22
PRIMER_LEFT_2_TM=59.801
PRIMER_RIGHT_2_TM=60.175
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=129
PRIMER_PAIR_PENALTY_3=0.3788
PRIMER_LEFT_3_PENALTY=0.332028
PRIMER_RIGHT_3_PENALTY=0.046801
PRIMER_LEFT_3_SEQUENCE=ACCTACTCCACGTCACTCTTCC
PRIMER_RIGHT_3_SEQUENCE=ACAGAGCACCTGAACAAACAGA
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=227,22
PRIMER_LEFT_3_TM=59.668
PRIMER_RIGHT_3_TM=59.953
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.3800
PRIMER_LEFT_4_PENALTY=0.199246
PRIMER_RIGHT_4_PENALTY=0.180765
PRIMER_LEFT_4_SEQUENCE=CTACTCCACGTCACTCTTCCCT
PRIMER_RIGHT_4_SEQUENCE=AGCACCTGAACAAACAGAACAA
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=223,22
PRIMER_LEFT_4_TM=59.801
PRIMER_RIGHT_4_TM=59.819
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_05602 Ps_contig_mira-and-tgicl-ass_franssen_38853
SEQUENCE=TAATTTCTTCCACTNGGNGGNGACATGCGTCCATTCTCCCTCCACACATGTTATTCTCCACTCCCAACGGTTTCTTTTCACTAGCCAATAAGACTCTCTTGATACAACCTTTGAGAGCATCCATGTGACTCTAAAATATTCATCTGAATGACAACTACAAATTCAAGGACCAACAATTAAAAGAGGAAAGGTGGTCATTTTGTAAAAGGAAGGAAATGTTAAGGTGAACAATCCACCAACTGCAACTACAAGAAAAAGGAAACAAATCCACTTTTTGAAAATTTCATTTTAAAATCTTTTCTCCTGTTCATTGTCTCCTCAACATCTCCGTCCGCCAATCTTCAACCATAAACCCATCCTGTTTTCTTTCTCTTCCTTACCACCAACTCCGACGAAACCTCACCCTCAACCATCCACCACCACAACAACCTCTTAAACATTGTGCCCCTTGCCGGGGAAAACCCACACCTCATCCACCTCTCAACCTCACTGGTACTCTAACAAAATCCGCCGTCGTCTCAACCTTACCGACGATTCAACAAAACCCGCCATAATTATGTTCTTCCACTTTCACATCTAAAATTCCGACCCACCACGTTAATAATCTTCTTCTTCTTCCATTTGTGACCCTTCATAACCTCACCGCCATACGCCTCATCTTTTTGTAAATGTTCACTTGCAGTGAGATTGGGTTTGAGAAGTGTTTGGTCATCAACATCTTAGTAGGACATTTCACATCGGATATTTGAGCAGCAAAACTCAACAGTTCAAACACTCATTTATTCATGCAGAGGATTAAATCATGGGTTGTCAAACATCCAACGTCATAGCAATCATTCAACGCAAGCTCAACATCTTAGTGTCGAGTTTCTCCGCTAATAACAACCATAATTAGTCAACATGACAGTAGTATTTTGAATCGATTTCTCCACCGCAACCACTTTCTTTCGCGTACCAGATATTGTTAACCATTCTCGTTTTTCATTTGTTGGGTATTTGTTAATTCATGAGTATTTGATGTTCTTCTGATTGAAAATAAAGTTTTAGTTTATTTTGATTTGCGTGAATATGAATTCTAGATTTCCTTATACTTGTTTGAATTCATGATAAATATAAAAATAAAGAAGGGTTGGACTG
TARGET=605,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1000
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.095629
PRIMER_LEFT_SEQUENCE=GTCTCAACCTTACCGACGATTC
PRIMER_RIGHT_SEQUENCE=CTGCTCAAATATCCGATGTGAA
PRIMER_LEFT=515,22
PRIMER_RIGHT=752,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.096
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.1000
PRIMER_LEFT_1_PENALTY=0.004359
PRIMER_RIGHT_1_PENALTY=0.095684
PRIMER_LEFT_1_SEQUENCE=GTCTCAACCTTACCGACGATTC
PRIMER_RIGHT_1_SEQUENCE=TGAACTGTTGAGTTTTGCTGCT
PRIMER_LEFT_1=515,22
PRIMER_RIGHT_1=769,22
PRIMER_LEFT_1_TM=60.004
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=0.1000
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.095684
PRIMER_LEFT_2_SEQUENCE=GTCTCAACCTTACCGACGATTC
PRIMER_RIGHT_2_SEQUENCE=AACTGTTGAGTTTTGCTGCTCA
PRIMER_LEFT_2=515,22
PRIMER_RIGHT_2=767,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.1000
PRIMER_LEFT_3_PENALTY=0.004359
PRIMER_RIGHT_3_PENALTY=0.095684
PRIMER_LEFT_3_SEQUENCE=GTCTCAACCTTACCGACGATTC
PRIMER_RIGHT_3_SEQUENCE=ACTGTTGAGTTTTGCTGCTCAA
PRIMER_LEFT_3=515,22
PRIMER_RIGHT_3=766,22
PRIMER_LEFT_3_TM=60.004
PRIMER_RIGHT_3_TM=60.096
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.1751
PRIMER_LEFT_4_PENALTY=0.004359
PRIMER_RIGHT_4_PENALTY=0.170703
PRIMER_LEFT_4_SEQUENCE=GTCTCAACCTTACCGACGATTC
PRIMER_RIGHT_4_SEQUENCE=CCCATGATTTAATCCTCTGCAT
PRIMER_LEFT_4=515,22
PRIMER_RIGHT_4=806,22
PRIMER_LEFT_4_TM=60.004
PRIMER_RIGHT_4_TM=60.171
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_05603 Ps_contig_mira-and-tgicl-ass_franssen_38856
SEQUENCE=TTGTCTCCTTCTTCTTCTATTATCTTTTTTTTCTTCTTTATCTTACTGTTCCCTTCTCCTTTTGTTTCCTTCTCTGCTTTCTTCTTCTTCTGTTCTCTACTCCTTTTTAGTCTCCATTTCTTATATTTATTTTCCTTGCGTGTGCTTGATGTATGGATGTCGAGGATGTGGGTTTTGAAGGTTTAGCTCAAGCTTCTTAAAGCTTCAATGAGAAGCTCTAGAAGCTAGGTTAATGAGCTTACGGTAAAGGTTTTGAAG
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3564
PRIMER_LEFT_PENALTY=0.321382
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=GTTCCCTTCTCCTTTTGTTTCC
PRIMER_RIGHT_SEQUENCE=AGCTAAACCTTCAAAACCCACA
PRIMER_LEFT=47,22
PRIMER_RIGHT=187,22
PRIMER_LEFT_TM=60.321
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.3662
PRIMER_LEFT_1_PENALTY=0.321382
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_LEFT_1_SEQUENCE=GTTCCCTTCTCCTTTTGTTTCC
PRIMER_RIGHT_1_SEQUENCE=TCCTCGACATCCATACATCAAG
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=165,22
PRIMER_LEFT_1_TM=60.321
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=119
PRIMER_PAIR_PENALTY_2=0.4100
PRIMER_LEFT_2_PENALTY=0.321382
PRIMER_RIGHT_2_PENALTY=0.088662
PRIMER_LEFT_2_SEQUENCE=GTTCCCTTCTCCTTTTGTTTCC
PRIMER_RIGHT_2_SEQUENCE=TAAACCTTCAAAACCCACATCC
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=184,22
PRIMER_LEFT_2_TM=60.321
PRIMER_RIGHT_2_TM=60.089
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=1.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=138
PRIMER_PAIR_PENALTY_3=0.4252
PRIMER_LEFT_3_PENALTY=0.390220
PRIMER_RIGHT_3_PENALTY=0.035021
PRIMER_LEFT_3_SEQUENCE=TGTTCCCTTCTCCTTTTGTTTC
PRIMER_RIGHT_3_SEQUENCE=AGCTAAACCTTCAAAACCCACA
PRIMER_LEFT_3=46,22
PRIMER_RIGHT_3=187,22
PRIMER_LEFT_3_TM=59.610
PRIMER_RIGHT_3_TM=60.035
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=142
PRIMER_PAIR_PENALTY_4=0.4351
PRIMER_LEFT_4_PENALTY=0.390220
PRIMER_RIGHT_4_PENALTY=0.044832
PRIMER_LEFT_4_SEQUENCE=TGTTCCCTTCTCCTTTTGTTTC
PRIMER_RIGHT_4_SEQUENCE=TCCTCGACATCCATACATCAAG
PRIMER_LEFT_4=46,22
PRIMER_RIGHT_4=165,22
PRIMER_LEFT_4_TM=59.610
PRIMER_RIGHT_4_TM=59.955
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_05604 Ps_contig_mira-and-tgicl-ass_franssen_38858
SEQUENCE=CCTTATTTCCCCTTTGTTTTGTTTTGTTTCCTTGCCTATCCCCTGTTGCGTGTCTCAGCTTGTAGAGCCTGTGCGAAGTAAGCACCGACCCGCTCCGGTGAAGTCCCGAACGGTGAGGAGAGTTCGGTTATCTCCGGTAAGAGATCATTAGCAAAGTCGAGGTTATCCATGGCAACACACTCAGCGCATTGGAGTAAAAGCCCTAGGAGTTTCAAACCAATGGATTCACCTTCAGTTGTAGGCTCAAACGCTTGCTCCTCGTCGTGTCCTTCGCCTTCCGCCACTGTCTTCTCGCCGGAGTAGTTAGGTCTTTTGAAAGATTGTACCGGAAGGGAAGAATCGGCGTCGTCGTGTTCGCGTTCAGGGCGTTTTGTGTTCATATTAGGTTTAGAAGTGAGAGGGGGTCGAGGGACCAAGCTTTGAAGCATGATCGATAAGAAGAGATAATGAGGGAGAAAATTTAGTTGAGAGAATCGAAAGGTTTGGAGGAAACTTTTGGGAGAAACGTGACAAAAATGAGAGGGAAAGA
TARGET=13,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05605 Ps_contig_mira-and-tgicl-ass_franssen_38875
SEQUENCE=TTATGAGGTCACTGAGATCCATTAGAGGGGATGGAATATCACTAGTGGCTGTATCTTTTACTTAGCAGTTGTTGAATCTCTTCTGCCTAACCCAACACTCCATGTTGGACCACTCAATGCTACAACAGAATCACGAGCAGCAGCAGCAACAATATCTGCACAAGAAACAATTCCAGGACAAGCAGTCTCCAACTGTGTTTTAATATTGTCAATAATATCAAAACCTCTAAGCGAATTCACATTTGCGCCCGCCGACTTCTCTCCGGTGAAGCTCGAAGTGTCATCCAACAGGACTGATGCATCACATCCGTTGAACAAAGCAATCGTGAAAATGAAGACGGAGCAACGAAGCGCCCATGCGACGCTCTTTGGAAACAGCGCTACATACTGCAGAGTTTATGATAGAGAGAGCTTTGGGACATGTTGT
TARGET=136,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0898
PRIMER_LEFT_PENALTY=0.081493
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=CTGAGATCCATTAGAGGGGATG
PRIMER_RIGHT_SEQUENCE=TGTCCTGGAATTGTTTCTTGTG
PRIMER_LEFT=11,22
PRIMER_RIGHT=179,22
PRIMER_LEFT_TM=59.919
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=0.1214
PRIMER_LEFT_1_PENALTY=0.081493
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=CTGAGATCCATTAGAGGGGATG
PRIMER_RIGHT_1_SEQUENCE=ACTGCTTGTCCTGGAATTGTTT
PRIMER_LEFT_1=11,22
PRIMER_RIGHT_1=185,22
PRIMER_LEFT_1_TM=59.919
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=175
PRIMER_PAIR_PENALTY_2=0.1386
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.008257
PRIMER_LEFT_2_SEQUENCE=ATCTCTTCTGCCTAACCCAACA
PRIMER_RIGHT_2_SEQUENCE=TGTCCTGGAATTGTTTCTTGTG
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=179,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=60.008
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=0.1702
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.039879
PRIMER_LEFT_3_SEQUENCE=ATCTCTTCTGCCTAACCCAACA
PRIMER_RIGHT_3_SEQUENCE=ACTGCTTGTCCTGGAATTGTTT
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=185,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=60.040
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=111
PRIMER_PAIR_PENALTY_4=0.1978
PRIMER_LEFT_4_PENALTY=0.189529
PRIMER_RIGHT_4_PENALTY=0.008257
PRIMER_LEFT_4_SEQUENCE=CTCTTCTGCCTAACCCAACACT
PRIMER_RIGHT_4_SEQUENCE=TGTCCTGGAATTGTTTCTTGTG
PRIMER_LEFT_4=77,22
PRIMER_RIGHT_4=179,22
PRIMER_LEFT_4_TM=59.810
PRIMER_RIGHT_4_TM=60.008
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_05606 Ps_contig_mira-and-tgicl-ass_franssen_38877
SEQUENCE=CCGTTACCTACCTTCYTGACAACAAACAAACAAACAAACAAGCAACAAGAATACCAACTCTTTCATTYCTTCTCCTTCTTTCACTTCTAAGAAATCTCATTCTCTCTCTCTAGGCAATGCAGGACAAGGTTTCAGTGTACATGTCATTGCTGTTGCTGCTGGTGAGGATGTTGGCCAGAAAATTATGCAATTTGTGCAACAAAATAGGGGTGAAATATGCATTCTGTCTGCATCTGGTTCCATCTCTAATGCCTCTCTTCGCCAACCGGCATCTTCAGGAGGCAACATTACGTATGAGGGTCGGTTTGATATTATTTCACTTACCGGTTCCTATGTCCGTAATGAAACTGGGGGGCGAAGTGGTGGTCTCAGTGTATGCTTGTCTAATTCTGATGGACAAATCATAGGCGGTGGTATTGGTGGGCCTCTTAAAGCTGCTGGTCCCGTTCAGGTCATTGTTGGTACATTTTCTGCCGACAACAAGAAGGATACTAGTGCTGGTGGAAAAGTGGATGCCTCTGCCGGTAAGTTGCCATCACCAGTTGGCGAATCAGCATCAAGTTTAGGTTTCCGCCAAACAGTTGACAGTTCCAGCGGGAATCCAATGCGGGTAAATGATGAACATCAAACCATGGGGGGAAGTCATTTCATGATTCAACAGCTTGGTTTGCATGTCACACCTCCCAGGTCCACAGACTGGGGAAGTCATCCAGATTCAAGACATGCTGGTTATGACCTATCAGGAAGAACAGGTCACGGGTCGCACCAGTCTCCTGAGAATGGAGGCTATGACCAAATTCCTGATTGAGAGTTTGAGATGACTTGTGAGTTTGAATAGCACCATTGTTTTGGTTTACCGTTGTATGATATCACTACATACCAGCAGTCTGTCACGTGATACTGTTTATAGTATGCACGCACCGTCCATTAAGGTCTCGGCTTCTGACATCTGAGAATTTATACTCATGTTTTGTCCATGAAAAATATTGTTTTGCCTCATTTACTCGTGTTGTATTTAAAAATTAGGATTGCCGGCTTATACTTCTTAATTTTTGTTGGGTCTGTTAGTTTGTCTATACCATTATAAATAAGGTATATTTACATAAA
TARGET=21,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05607 Ps_contig_mira-and-tgicl-ass_franssen_38877
SEQUENCE=CCGTTACCTACCTTCYTGACAACAAACAAACAAACAAACAAGCAACAAGAATACCAACTCTTTCATTYCTTCTCCTTCTTTCACTTCTAAGAAATCTCATTCTCTCTCTCTAGGCAATGCAGGACAAGGTTTCAGTGTACATGTCATTGCTGTTGCTGCTGGTGAGGATGTTGGCCAGAAAATTATGCAATTTGTGCAACAAAATAGGGGTGAAATATGCATTCTGTCTGCATCTGGTTCCATCTCTAATGCCTCTCTTCGCCAACCGGCATCTTCAGGAGGCAACATTACGTATGAGGGTCGGTTTGATATTATTTCACTTACCGGTTCCTATGTCCGTAATGAAACTGGGGGGCGAAGTGGTGGTCTCAGTGTATGCTTGTCTAATTCTGATGGACAAATCATAGGCGGTGGTATTGGTGGGCCTCTTAAAGCTGCTGGTCCCGTTCAGGTCATTGTTGGTACATTTTCTGCCGACAACAAGAAGGATACTAGTGCTGGTGGAAAAGTGGATGCCTCTGCCGGTAAGTTGCCATCACCAGTTGGCGAATCAGCATCAAGTTTAGGTTTCCGCCAAACAGTTGACAGTTCCAGCGGGAATCCAATGCGGGTAAATGATGAACATCAAACCATGGGGGGAAGTCATTTCATGATTCAACAGCTTGGTTTGCATGTCACACCTCCCAGGTCCACAGACTGGGGAAGTCATCCAGATTCAAGACATGCTGGTTATGACCTATCAGGAAGAACAGGTCACGGGTCGCACCAGTCTCCTGAGAATGGAGGCTATGACCAAATTCCTGATTGAGAGTTTGAGATGACTTGTGAGTTTGAATAGCACCATTGTTTTGGTTTACCGTTGTATGATATCACTACATACCAGCAGTCTGTCACGTGATACTGTTTATAGTATGCACGCACCGTCCATTAAGGTCTCGGCTTCTGACATCTGAGAATTTATACTCATGTTTTGTCCATGAAAAATATTGTTTTGCCTCATTTACTCGTGTTGTATTTAAAAATTAGGATTGCCGGCTTATACTTCTTAATTTTTGTTGGGTCTGTTAGTTTGTCTATACCATTATAAATAAGGTATATTTACATAAA
TARGET=101,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05608 Ps_contig_mira-and-tgicl-ass_franssen_38902
SEQUENCE=CCTTTCCTACACCCTTTCCATTAACTAACGAAACTCCTTCCCNTTAAAAACTTACTTTACCATTTTTTCTACGCAGCTTTTCACCAAATTTTATATTCAAACAGCTAGGTTGAATTGCTTTGGGATGATGATGATGACTTGTAGCCAAAGACGACGAATTTTCAAACACCTTCAAGGCATGAATCAGAGAAAGCTTATCCTTTTCAAGATGAGAAAGAGAAGAAGAAATTGAATTGAAAAGCTCTAGCAAAAACAAACTGTAGCTGAGATACTCTTCAATAGAATCAACCTCCCATTTACTCATTGGATAATCTATCTCCAAAGTAAGGTTTTCAAAAGTCTTGTTGATCAAGGGTAAGATTCCAAGGCATTTTTGAATCCAAAACAAGGATAGTATTTCTGATCCGGGTTTCGAATTTGATCCTAATTCGTTGATGAAATTCGAAACTTGGAATCGAAAAGCCTTCAGGGATGCTGATAAGTCCTCAGCTTCATGGTGATGATGATCATGATTCTCCAGCTTGTTGTAGAGTTTTGCAAATTTCTGACCTAAGAGGGCTACCATGGTTTATAACAAATAACAACACAAAAAGAAGATGTATAACTTTGTGGGGTGTGTGTTTTTCAACTAGAATTCCNNNAACTAAAAAAGGTTTTGAAATTGGTTTGTTTTATTATTATT
TARGET=124,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7927
PRIMER_LEFT_PENALTY=0.593677
PRIMER_RIGHT_PENALTY=0.199067
PRIMER_LEFT_SEQUENCE=TCAAACAGCTAGGTTGAATTGC
PRIMER_RIGHT_SEQUENCE=AATGCCTTGGAATCTTACCCTT
PRIMER_LEFT=96,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=59.406
PRIMER_RIGHT_TM=60.199
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.8899
PRIMER_LEFT_1_PENALTY=0.593677
PRIMER_RIGHT_1_PENALTY=0.296177
PRIMER_LEFT_1_SEQUENCE=TCAAACAGCTAGGTTGAATTGC
PRIMER_RIGHT_1_SEQUENCE=TCCAATGAGTAAATGGGAGGTT
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=307,22
PRIMER_LEFT_1_TM=59.406
PRIMER_RIGHT_1_TM=59.704
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=212
PRIMER_PAIR_PENALTY_2=0.8903
PRIMER_LEFT_2_PENALTY=0.562203
PRIMER_RIGHT_2_PENALTY=0.328094
PRIMER_LEFT_2_SEQUENCE=CCTTTCCTACACCCTTTCCATT
PRIMER_RIGHT_2_SEQUENCE=AAAATTCGTCGTCTTTGGCTAC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=161,22
PRIMER_LEFT_2_TM=60.562
PRIMER_RIGHT_2_TM=59.672
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=162
PRIMER_PAIR_PENALTY_3=0.9225
PRIMER_LEFT_3_PENALTY=0.562203
PRIMER_RIGHT_3_PENALTY=0.360320
PRIMER_LEFT_3_SEQUENCE=CCTTTCCTACACCCTTTCCATT
PRIMER_RIGHT_3_SEQUENCE=GATTCATGCCTTGAAGGTGTTT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=184,22
PRIMER_LEFT_3_TM=60.562
PRIMER_RIGHT_3_TM=60.360
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=185
PRIMER_PAIR_PENALTY_4=0.9548
PRIMER_LEFT_4_PENALTY=0.562203
PRIMER_RIGHT_4_PENALTY=0.392624
PRIMER_LEFT_4_SEQUENCE=CCTTTCCTACACCCTTTCCATT
PRIMER_RIGHT_4_SEQUENCE=GGTGTTTGAAAATTCGTCGTCT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=169,22
PRIMER_LEFT_4_TM=60.562
PRIMER_RIGHT_4_TM=60.393
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_05609 Ps_contig_mira-and-tgicl-ass_franssen_38918
SEQUENCE=TGAACAAAATCATGTAACCGATCGTACTAACATCAAGGCAGTTTTACTAGACATGATTCTAGCTGGAATTGATACATCAGCTACCGTAATTGAGTGGGTGTTATCCGAGCTTTTAAGAAATCCAAGAGTCATGAAAAATCTTCAAGATGAAATACAAAATGAAGTTGGAGATAGAAGAATAGTTAAGGAGAAAGATTTGAAGAATTTAAGTTATTTAGATATGGTTGTTGATGAGATATTAAGACTATATCCTGTTGGACCTTTATTAGTTCCTCGTGAATGTAGAGAAAATATTACGGTCGATGGTTACTTTATAAGGGAAAAAACACGAGTTATAGTAAATGCATGGGCAATAGGGAGAGATCATGATGTTTGGTCACAAAATGCAGAACAATTTTATCCTGAAAGATTTATTGACAAGAAAATGAATTTTCATGGACAAGAATTTGAGTGTATACCATTTGGTTCTGGTCGGAGACGTTGTCCTGGAATTCATTTGGGATTGGTTACAGTCAAATTTGCTATAGCTCAATTGGTGCATTGTTTTAATTGGAAACTTCCTCATAATATAACTCCTTTCAATTTGAGCATGGAAGAGAAATTTGGACTCACAATGCCAAGAGCTCAACATCTGTATGCAATACCAAGTTATCGTTTGGCAAGTGATGGAGAATTTGAATAGTTTCAAGAAGGACAATTAAATGTCACCATGAAAAACCTGAGTTCTTTTACAATTAATTAATTAAGTAAAGTGTTGGTTNTTTACATATGATAAATAGCCAAAATTGAATGTATTGGAATAAAAGCTTACTATTTATGTGCTTAGTTRTGTATTTATTGTAATAATTGAAAAGATAATGTATTCAAATTGTTTTTATATGATAATGAT
TARGET=733,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05610 Ps_contig_mira-and-tgicl-ass_franssen_38933
SEQUENCE=GGTAATACTAATTTTGGTGCTTTATTTTATTTTTTAAATTTAAATCAGATGAAAACAAAAATAAATAAATAAAAAATAAATTACATTCACTTCAATTCTTCTTGCCTTCTTCTCCATCATTTTCCACGTAAGCTTTCTTTCCTTCTATCCGCCATCGACAAAAAAGAACAAATCNAGTAAAAAACTCGTGGCATAAATCCCGTGAATCTTCCAATTACTCTTCGGCGGTTGCCGCAGCACCACCCTGTGAAACACCGTCACGTAGTAATGAAGCCCAACAGAAAAGCCCACAAAGATCTGGGCCAGGCCCAAGAACTTTGAACCAAGCCCACATTCAGTAGAGAAGACAAGAACAAGGTACATGACGAGTAACAGTAGATAGAAAACGTAACCGAGAGTTTGAAGCACTTGGAAGCAGATATACGTGGACTGCGAAGTAGAGAATAGAAACTTCAAGGGTTCAGTTCCAGTGACCAATGTTGAGATACAGAGAATGGTGAAGTAGATTGAGAGGTAAATTTGGATGAAGATTAGGATTTTTTGAAGTGAGAAATAGGTTTTGACTCTGTTGCAAAGGAGAGAGACTAAGAGGAGAGGGAGTAATACGAAAGCGGTAAGAAACTATGGCGGCTATGGTGGGTGCGTATTTTTTGCCGACGTAGGGTTTGAATCCTTTGGTCATTTCGTTGTTGGCGTTTGTTATGTAGGCTTTGGAGCTTGTTGA
TARGET=59,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05611 Ps_contig_mira-and-tgicl-ass_franssen_38938
SEQUENCE=ATCCTCCTCCTTAACAGCATTACTAAACATTTTATCATCATCATCACTACCATCAATCTTTTGATTAACCAAATCAATATGCTTGATGATATTACTCGATTTAAACGGGACAAAACGGTCATCTTTGTCCTTTAGATTCGAGCACAAAAGGCTTCGGAACTCTTTCTCTAGATCGAAAGTTTTCGTGAGCCAGCCTTCGAAACCAATCAGATATTTATCATAAATCAATTTAATCGAAGCCAAAAAAATGAAATTCCAAACCCAGCTCTACTATAACAGAATCCCACAACCCTTTTCTCGATACCACATCATACCCACCGTTTTCTTTCACAAGAGAGAAAAGTTGGTGTAAATCAACCAATCGACCGTCACCAAGCACCACCGGAACAGGCCTCACATTACCTCCACTACGATCCTCCTTAAGGTAAACCGAAAAAATTTAGACAAAACAAAGACTTTTGTTTCGCTTTGTCATAATCAGCGCTATCAACCCGATCCT
TARGET=34,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5750
PRIMER_LEFT_PENALTY=2.479568
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=TCCTCCTCCTTAACAGCATTAC
PRIMER_RIGHT_SEQUENCE=TATCTGATTGGTTTCGAAGGCT
PRIMER_LEFT=1,22
PRIMER_RIGHT=212,22
PRIMER_LEFT_TM=57.520
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=2.5968
PRIMER_LEFT_1_PENALTY=2.479568
PRIMER_RIGHT_1_PENALTY=0.117281
PRIMER_LEFT_1_SEQUENCE=TCCTCCTCCTTAACAGCATTAC
PRIMER_RIGHT_1_SEQUENCE=TGTGCTCGAATCTAAAGGACAA
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=145,22
PRIMER_LEFT_1_TM=57.520
PRIMER_RIGHT_1_TM=59.883
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=2.6062
PRIMER_LEFT_2_PENALTY=2.510759
PRIMER_RIGHT_2_PENALTY=0.095445
PRIMER_LEFT_2_SEQUENCE=TCCTCCTCCTTAACAGCATTACT
PRIMER_RIGHT_2_SEQUENCE=TATCTGATTGGTTTCGAAGGCT
PRIMER_LEFT_2=1,23
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=58.489
PRIMER_RIGHT_2_TM=60.095
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=2.6280
PRIMER_LEFT_3_PENALTY=2.510759
PRIMER_RIGHT_3_PENALTY=0.117281
PRIMER_LEFT_3_SEQUENCE=TCCTCCTCCTTAACAGCATTACT
PRIMER_RIGHT_3_SEQUENCE=TGTGCTCGAATCTAAAGGACAA
PRIMER_LEFT_3=1,23
PRIMER_RIGHT_3=145,22
PRIMER_LEFT_3_TM=58.489
PRIMER_RIGHT_3_TM=59.883
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=2.8625
PRIMER_LEFT_4_PENALTY=2.479568
PRIMER_RIGHT_4_PENALTY=0.382951
PRIMER_LEFT_4_SEQUENCE=TCCTCCTCCTTAACAGCATTAC
PRIMER_RIGHT_4_SEQUENCE=AGCCTTTTGTGCTCGAATCTAA
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=152,22
PRIMER_LEFT_4_TM=57.520
PRIMER_RIGHT_4_TM=60.383
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_05612 Ps_contig_mira-and-tgicl-ass_franssen_38951
SEQUENCE=CGAACCCAACCTCCTATCTTGTAAGTAACTAAGAATTTAATTTGTAAGGGTTTAGGTCGATATGAAATAAGAGCAGCAAATTCCTATGCTTTTGGTTTGAGAGACAAGCGCTGAATGAAGCCATGGAAGGGGAAAAGAATGGTCATCACCACTTACCTGAAACTCGAGGACTTTGCTTGTCAGAAGAGCAAACTATTCCCACTATTTTAAGGAGGCCGAGTATAGGAGCAGGTTACCGTGTCATAGATATGATAACCGAGCCTTTTAGGCTGATACGTCGGTGCGAAGTGACAGCAATTCTTGTATTGTATGGCCTTCCTAGGTTACTGACAGGATCAATCCTAGCTCATGAAATGATGCATGCTTGGCTTAGACTTAAAGGCTATGGCAATCTTAGGCCAGAAGTTGAAGAAGGAATATGCCAAGTCTTAGCTCACATGTGGTTAGATTCAGAGATCTATTCCGGCTCCGGAAGCGAAGAAGCATCATCATCATCTTCATCTTCGTCGTCTTCATCATCACCTTCCTCGACTTCATCAAAGAAGGGTAAACGGTCGGATTTTGAAAAGGAACTCGGTAAATTTTTTAAACACCAGATCGAGTCAGATAGCTCACCGGCCTATGGAGATGGATTCAGAGAGGGTAACCAGGCAGTTCTTAAGTACGGACTTAGAAGAACCCTCGATCATATCCGAATCACCGGAAGTTTTCCGTAAAGAAATGGTGATATAAATATTATTTTCTTACCTTTACATCAACCATAAGATTTGAGCATTGTCACATAAACTAAATCCATAAACTATATAGGAACCAAATATTTCAAAATTGTATAATTTCACCAAACTCCACACAAAATAAGGATTGAAAATGCAAGTGATAGTGTAAAGTTACTGTAATCTATACCTAAATATTGGCAGTTTGGCACCTCATGCTACATATGTATCACAAAATACCTTATAGAAGTTGAAAACAAAACAGTTGATGCAATTATAGATATACAATAAAGAACCAATTTTTGT
TARGET=484,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1740
PRIMER_LEFT_PENALTY=0.113803
PRIMER_RIGHT_PENALTY=0.060196
PRIMER_LEFT_SEQUENCE=TTAGGCCAGAAGTTGAAGAAGG
PRIMER_RIGHT_SEQUENCE=CTCTGAATCCATCTCCATAGGC
PRIMER_LEFT=393,22
PRIMER_RIGHT=638,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=60.060
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=246
PRIMER_PAIR_PENALTY_1=0.2424
PRIMER_LEFT_1_PENALTY=0.113803
PRIMER_RIGHT_1_PENALTY=0.128591
PRIMER_LEFT_1_SEQUENCE=TTAGGCCAGAAGTTGAAGAAGG
PRIMER_RIGHT_1_SEQUENCE=ACCGTTTACCCTTCTTTGATGA
PRIMER_LEFT_1=393,22
PRIMER_RIGHT_1=554,22
PRIMER_LEFT_1_TM=59.886
PRIMER_RIGHT_1_TM=59.871
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.2872
PRIMER_LEFT_2_PENALTY=0.113803
PRIMER_RIGHT_2_PENALTY=0.173354
PRIMER_LEFT_2_SEQUENCE=TTAGGCCAGAAGTTGAAGAAGG
PRIMER_RIGHT_2_SEQUENCE=TGGTTACCCTCTCTGAATCCAT
PRIMER_LEFT_2=393,22
PRIMER_RIGHT_2=648,22
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=59.827
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.3361
PRIMER_LEFT_3_PENALTY=0.113803
PRIMER_RIGHT_3_PENALTY=0.222249
PRIMER_LEFT_3_SEQUENCE=TTAGGCCAGAAGTTGAAGAAGG
PRIMER_RIGHT_3_SEQUENCE=CGAGGGTTCTTCTAAGTCCGTA
PRIMER_LEFT_3=393,22
PRIMER_RIGHT_3=683,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=59.778
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.3626
PRIMER_LEFT_4_PENALTY=0.113803
PRIMER_RIGHT_4_PENALTY=0.248820
PRIMER_LEFT_4_SEQUENCE=TTAGGCCAGAAGTTGAAGAAGG
PRIMER_RIGHT_4_SEQUENCE=CTTTGATGAAGTCGAGGAAGGT
PRIMER_LEFT_4=393,22
PRIMER_RIGHT_4=541,22
PRIMER_LEFT_4_TM=59.886
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_05613 Ps_contig_mira-and-tgicl-ass_franssen_38958
SEQUENCE=CTTTGTTCCATTTCTGGTTATGAAACCGATCACACTTTGATCTCTTCTTGCATATCCCTCCTCTCTCTCTCTCTCCAATATCTTTCTCCAAATCCCACTGAAAATGAGGAACAGAATCTACCATCTCTTCCCAGGTGATTTTTAGCATTCACCACCAACTATCCTTCCAAAAACCACCTTTTTCACCTTCTTCCAACCCTACACTACAAACACTCCTTTCCCCTCTTAGGTGCTTCCCTAATAGCGTTCTTTTCCACAAATATTTTCTTTCTCAAGTTACAAAACATAACCATAACCTTTCCTTTATCTTTAAAGCCAACCCTAGCTAAAAAAAGAACCATATATACATAAATATTTATGGAAGGTTTTCACCCATCTATGAATATGAGCATGAGCATGAGCATGAACATGAACCCTTCTTTTTCATATACTTTCCCTATCACTGCTACTGCTTCTGGTGCTATCACAAATATTACTACTACCACCACCACTTACAACACTACCAGTACTACTCCATGGATGAACAGCAGGATATGGAGTAAGCTTCCTCACAGACTACTTGATCGCATCATTGCTTTCCTTCCTCCACCTGCTTTCTTCCGTGCACGTGCTGTCTGCAAGAGATTTTACTCTCTTCTCTTTTCCAACTCTTTCCTTGAATTATACCTTCAAGTTTCACCTCGTTTCCACTGGTTCATATTCTTCAAACATAAAACCAGATCAAAAACCCACATCTACAAAAACAACACCATCACTGATAATAATTCCTTTGAAGGCTACATCTTTGATCCTAATGAAGTGGCATGGTACCGTATTTCCTTCGCTTTAATCCCTTCTGGTTTCTCACCATCTTCTTCATCTGCTGGTTTACTATGCTGGGTTTCTGATGAGTCCGGTCCAAAAACAATGCTTCTCTCAAACCCGATCCTCGGTTCCATTACTCAGTTACCACCAACACTAAGACCAAGACTCTTCCCTTCAATAGGTTTAACCATTACTCCATCTTCCATAGATGTTACTGCTGCAGGTGATGACATGATATCACCTTATGCAGTCAAAAACCTATCATCTGAAAGCTTTCATATAGATGCAAGTGGGTTTTACTCCATCTGGGGAACAACCTCTTCATTACCAAGACTCTGTAGTCTTGAATCAGGTAGAATGGTTTATTCACAAGGGAAATTTTACTGCATGAACTGTAGTCCTTTCAGTGTTTTAGCTTATGATATAGCTACAAACACCTGGTTCAAAATTCAAGCTCCTATGAGGAGGTTTTTAAGGTCACCTAACTTGGTTGAATGCAAAGGAAAGTTGTTGCTTGTTGCTGCTGTTGAGAAGAGTAAGCTGAATGTTCCAAAGAGTTTGAGGGTCTGGAGTTTGCAAGGCTGTGGAAGTGTGTGGGTGGAAACGGAGAGAATGCCACAACAACTTTATGTTCAGTTTGCTGATATGGAAAATGGGAATGGATTTGAGTGTGTTGGGAATGGTGAGTTTATTGTGATAATGATAAAAGGGAGTGATAAGGGTTTGGTGTATGATATAGGAAGGAAGAGGTGGCAGTGGATTCCACCATGTCCTTATGCTGGTTATGATGGGTTTGAGTTGCATGGTTTTGCTTATGATCCTAGGTTGGCAACACCTGTTACTGCTCTACTTGATCAGTTGGCAATGCCTCTTCCGCAGTTTTAATTCACAAATGCTTCTGAAATAGTATAGGGGTTATCATATGAAAGTATAATGTTTAATTAATGATGGTATGGTATGTGTAGTGATTAATATGTATTTGAATGAATGTCTCGTGTTGTGTATTAGCAATGATCAGTTTAATAACTTAAACTGTTAATCTTGGTTGTTTTGAATCTTAATGCAATATGATTTTAT
TARGET=61,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3696
PRIMER_LEFT_PENALTY=0.365170
PRIMER_RIGHT_PENALTY=0.004430
PRIMER_LEFT_SEQUENCE=TCTGGTTATGAAACCGATCACA
PRIMER_RIGHT_SEQUENCE=CCTAAGAGGGGAAAGGAGTGTT
PRIMER_LEFT=12,22
PRIMER_RIGHT=229,22
PRIMER_LEFT_TM=60.365
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=0.3801
PRIMER_LEFT_1_PENALTY=0.365170
PRIMER_RIGHT_1_PENALTY=0.014945
PRIMER_LEFT_1_SEQUENCE=TCTGGTTATGAAACCGATCACA
PRIMER_RIGHT_1_SEQUENCE=ATTAGGGAAGCACCTAAGAGGG
PRIMER_LEFT_1=12,22
PRIMER_RIGHT_1=241,22
PRIMER_LEFT_1_TM=60.365
PRIMER_RIGHT_1_TM=59.985
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.3913
PRIMER_LEFT_2_PENALTY=0.365170
PRIMER_RIGHT_2_PENALTY=0.026163
PRIMER_LEFT_2_SEQUENCE=TCTGGTTATGAAACCGATCACA
PRIMER_RIGHT_2_SEQUENCE=TAGGGTTGGAAGAAGGTGAAAA
PRIMER_LEFT_2=12,22
PRIMER_RIGHT_2=200,22
PRIMER_LEFT_2_TM=60.365
PRIMER_RIGHT_2_TM=59.974
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.4539
PRIMER_LEFT_3_PENALTY=0.365170
PRIMER_RIGHT_3_PENALTY=0.088755
PRIMER_LEFT_3_SEQUENCE=TCTGGTTATGAAACCGATCACA
PRIMER_RIGHT_3_SEQUENCE=TGGAAAAGAACGCTATTAGGGA
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=60.365
PRIMER_RIGHT_3_TM=60.089
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.4645
PRIMER_LEFT_4_PENALTY=0.365170
PRIMER_RIGHT_4_PENALTY=0.099308
PRIMER_LEFT_4_SEQUENCE=TCTGGTTATGAAACCGATCACA
PRIMER_RIGHT_4_SEQUENCE=GGGGAAAGGAGTGTTTGTAGTG
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=222,22
PRIMER_LEFT_4_TM=60.365
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=211
=
PRIMER_SEQUENCE_ID=SSR_05614 Ps_contig_mira-and-tgicl-ass_franssen_38959
SEQUENCE=AGTAAATGAACACATCTCATCACCCTAATCATATCACACATTCAGTAACGGCAACATCAACTCATACTCATAATCATGCTCAACATAAACATCAAAACACACGTATAATATTGGAATACATCCATTCATATTATACGCCATACATACATATATTATGCAATGAGACTCCATGCA
TARGET=139,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1020
PRIMER_LEFT_PENALTY=0.175676
PRIMER_RIGHT_PENALTY=0.926360
PRIMER_LEFT_SEQUENCE=TCAGTAACGGCAACATCAACTC
PRIMER_RIGHT_SEQUENCE=GCATGGAGTCTCATTGCATAAT
PRIMER_LEFT=41,22
PRIMER_RIGHT=172,22
PRIMER_LEFT_TM=60.176
PRIMER_RIGHT_TM=59.074
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=1.2497
PRIMER_LEFT_1_PENALTY=0.323355
PRIMER_RIGHT_1_PENALTY=0.926360
PRIMER_LEFT_1_SEQUENCE=TTCAGTAACGGCAACATCAACT
PRIMER_RIGHT_1_SEQUENCE=GCATGGAGTCTCATTGCATAAT
PRIMER_LEFT_1=40,22
PRIMER_RIGHT_1=172,22
PRIMER_LEFT_1_TM=59.677
PRIMER_RIGHT_1_TM=59.074
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=133
PRIMER_PAIR_PENALTY_2=1.3546
PRIMER_LEFT_2_PENALTY=0.428205
PRIMER_RIGHT_2_PENALTY=0.926360
PRIMER_LEFT_2_SEQUENCE=CACATTCAGTAACGGCAACATC
PRIMER_RIGHT_2_SEQUENCE=GCATGGAGTCTCATTGCATAAT
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=172,22
PRIMER_LEFT_2_TM=60.428
PRIMER_RIGHT_2_TM=59.074
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=1.3546
PRIMER_LEFT_3_PENALTY=0.428205
PRIMER_RIGHT_3_PENALTY=0.926360
PRIMER_LEFT_3_SEQUENCE=ACATTCAGTAACGGCAACATCA
PRIMER_RIGHT_3_SEQUENCE=GCATGGAGTCTCATTGCATAAT
PRIMER_LEFT_3=37,22
PRIMER_RIGHT_3=172,22
PRIMER_LEFT_3_TM=60.428
PRIMER_RIGHT_3_TM=59.074
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=5.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=1.3546
PRIMER_LEFT_4_PENALTY=0.428205
PRIMER_RIGHT_4_PENALTY=0.926360
PRIMER_LEFT_4_SEQUENCE=ATCACACATTCAGTAACGGCAA
PRIMER_RIGHT_4_SEQUENCE=GCATGGAGTCTCATTGCATAAT
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=172,22
PRIMER_LEFT_4_TM=60.428
PRIMER_RIGHT_4_TM=59.074
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=5.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_05615 Ps_contig_mira-and-tgicl-ass_franssen_38978
SEQUENCE=ATATCACCCACCAACACAACAAAATACTCTATTCCGGAAGCTCTTTGGTTCGATCCCGAAGATCTTTCACTTTCTCTGTGAATTCATCGAACGGTAAAGAAGGATCAGGGAGAAAACTTTGTGGCTGCCAGCATTGTTCCACGGGCTTTAGCAGGGGCAATACAGACTCAGAAGCCCAGGTTTCTAGTGACTTGAAAATTTCGAGTTTTTCCGGTGGCAATGAGTGGGTTTTGGAGAGTTTTAGTGATGGTGGCGCGGCGGAGACGGTGGATTTCGGTGGTGGAAGTAGGTGGCGATGTGTTCGGTTGTTGATGGTTTTTGGAGTGTTCCATGTTAGTGGAAGTGTTTTAACGGAGGGRCAAGTTTGAATATGCATGGTTTTGTTTTGTTTTGTTTTGTGTTGTTTGTTCCCCGCG
TARGET=378,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05616 Ps_contig_mira-and-tgicl-ass_franssen_38984
SEQUENCE=TTCGTAAGAGAAGCAAACTGCGTTTATTTAATTATAGTACACACCAACATAGTAGTGACTGACACTAGACATTTACATTCTACTAGTAGTACTTATAGTACTACTAATCACCACCACACATTTACATACTACAAAGTACTTATTTATGGAACTAAGTTAAAACGCAGTAGTAACAAGTAGATGAAATAGGTAGCACTATTTGCTGCCTTTGTCCGCAGCATGAAGATCACTGAAGTATCCCTGAGGGTTGCTTTCAAATGCTCCACGAGATATATATGATCCACCACCACCCGGGGCTTTCATTGAACCACCACCACCACCACCACCGCTTCCACCACCGCCTCCTTTTGCATCTCCACCACCGCCTCCTTTAGCCCCACCACCTCCACCGCCTCCTTTAGCTCCACCACCTCCACCGCCTCCTTTAGCTCCACCACCGCTACCACCTTTGCCACCCATGTTTCACAAAGACAAGTTAAAATACTATAAGATAATTTTCTAAGAAAAGGATAGTACCCCCC
TARGET=307,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9977
PRIMER_LEFT_PENALTY=0.180916
PRIMER_RIGHT_PENALTY=0.816764
PRIMER_LEFT_SEQUENCE=GGTAGCACTATTTGCTGCCTTT
PRIMER_RIGHT_SEQUENCE=TTGTCTTTGTGAAACATGGGTG
PRIMER_LEFT=188,22
PRIMER_RIGHT=473,22
PRIMER_LEFT_TM=59.819
PRIMER_RIGHT_TM=60.817
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=1.1765
PRIMER_LEFT_1_PENALTY=0.180916
PRIMER_RIGHT_1_PENALTY=0.995578
PRIMER_LEFT_1_SEQUENCE=GGTAGCACTATTTGCTGCCTTT
PRIMER_RIGHT_1_SEQUENCE=ACTTGTCTTTGTGAAACATGGG
PRIMER_LEFT_1=188,22
PRIMER_RIGHT_1=475,22
PRIMER_LEFT_1_TM=59.819
PRIMER_RIGHT_1_TM=59.004
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=1.1765
PRIMER_LEFT_2_PENALTY=0.180916
PRIMER_RIGHT_2_PENALTY=0.995578
PRIMER_LEFT_2_SEQUENCE=GGTAGCACTATTTGCTGCCTTT
PRIMER_RIGHT_2_SEQUENCE=CTTGTCTTTGTGAAACATGGGT
PRIMER_LEFT_2=188,22
PRIMER_RIGHT_2=474,22
PRIMER_LEFT_2_TM=59.819
PRIMER_RIGHT_2_TM=59.004
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=1.4425
PRIMER_LEFT_3_PENALTY=0.180916
PRIMER_RIGHT_3_PENALTY=1.261614
PRIMER_LEFT_3_SEQUENCE=GGTAGCACTATTTGCTGCCTTT
PRIMER_RIGHT_3_SEQUENCE=GTCTTTGTGAAACATGGGTGG
PRIMER_LEFT_3=188,22
PRIMER_RIGHT_3=471,21
PRIMER_LEFT_3_TM=59.819
PRIMER_RIGHT_3_TM=60.262
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=1.4557
PRIMER_LEFT_4_PENALTY=0.638917
PRIMER_RIGHT_4_PENALTY=0.816764
PRIMER_LEFT_4_SEQUENCE=CTTTCAAATGCTCCACGAGATA
PRIMER_RIGHT_4_SEQUENCE=TTGTCTTTGTGAAACATGGGTG
PRIMER_LEFT_4=250,22
PRIMER_RIGHT_4=473,22
PRIMER_LEFT_4_TM=59.361
PRIMER_RIGHT_4_TM=60.817
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_05617 Ps_contig_mira-and-tgicl-ass_franssen_38985
SEQUENCE=CCGGTAAAAAAACTTTAAAAAATTTAGGTACCCGGTTCCGTTCCTTGNAAGGCATTATGACTGATGAGCTGAGAACTGTTTTGGAGACTAGCTTACCCAAAGTAAAGGAAGGTAAGAAGCCAAAGTTCTCCTTAGGTGTAGCTGAATCTAAGATTGGTTCACATATACAAGATGCTACTAAAATTCCATGCCAAAGTAATGAGTTTGTGAATGAACTTATTCGTGGTGTCAGGCTTCATTTTGACAAGTTTGTTGGTGATCTCAAGCCAGGAGATTTGGAGAAGGCGCAACTTGGTCTCTGCCACAGTTACAGCAGAGCAAAAGTGAAATTCAATGTTAATCGGGTTGACAATATGGTCATCCAAGCAATCTTCCTACTCGATACACTTGATAAGGATATCAATTCATTTGCCATGAGAGTCAGAGAATGGTATTCATGGCATTTTCCTGAACTAGTGAAGATCATAAATGATAATTATCTGTATTGCAAAGTAGCCAAATATGTTGAGGATAAGTCAAAGTTGGCTGAAGACAATATTGAAGCATTAACTGACTTAGTTGGAGACGAAGATAAAGCAAAGGAGATTGTTGAAGCAGCAAAAGCCTCCATGGGACAAGAATTGTCCCCTGTGGACTTGATCAATGTCCATCAGTTTGCTCAAAGGGTGATGGACCTATCTGACTACAGGAGAAGGTTGTCTGATTACCTGACTACTAAGATGAATGATATTGCACCTAATTTGCAATCTTTAGTGGGTGATAGTGTTGGAGCTCGTTTAATTTCCCATGCTGGTAGTCTCACAAATTTAGCTAAGTGCCCGTCATCAACTCTTCAGATTCTTGGTGCAGAGAAAGCTTTATTCAGGGCATTAAAAACAAGAGGAAATACTCCCAAATATGGTCTGATATTCCACTCTTCTTTTATTGGTCGAGCATCTGCCAAAAACAAGGGCCGCATGGCTCGCTATCTTGCAAACAAATGCTCTATTGCATCTAGGATTGACTGCTTTTCTGAAAAGGGTACTTCAGCATTTGGTGAGAAACTCCGTGAGCAAGTTGAGGAGCGACTTGACTTTTATGATAAGGGAGTTGCCCCTCGTAAGAACATTGATGTCATGAAATCTGCTATTGAAGTTGCCGACAACATAGATACAGAGATGGAGACAGAGGAAGCATCAGCCAAGAAAACCAAGAAGAAGAAGAAGCAAAAGGCTGGTGAAGATGAAGGAGAAGACTTAGCTGTTGAAAAATCATCAGAAATTACTAATGGTGATGCAGAGGATCATAAGTCAGAAAAGAAGAAGAAAAAGAAAGAAAAGAGAAAATTGGACCAGGAGGTAGATGTTCAAGATCAGATTGTTGATGAAGCTGCCAATGGCGAGTCTGGAAAGAAGAAGAAGAAGAAGTCAAAGAGGAAGGATGTCGATTAAAACTAAACTAGACCTTAAACTATGCTTGCTGTATTGGATGTTGTTATATTTTAGTTTTGTCTTTGAGTAACTTTNTAATTTNRTTGTTCTTAGTAACTTCTTTTATTTATTTNTTTTTTGTTTAGTTATCTATAGTACTTTAAATTTTTGTCGTAACTTTAGTTTAAAAAACGGT
TARGET=1191,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05618 Ps_contig_mira-and-tgicl-ass_franssen_38985
SEQUENCE=CCGGTAAAAAAACTTTAAAAAATTTAGGTACCCGGTTCCGTTCCTTGNAAGGCATTATGACTGATGAGCTGAGAACTGTTTTGGAGACTAGCTTACCCAAAGTAAAGGAAGGTAAGAAGCCAAAGTTCTCCTTAGGTGTAGCTGAATCTAAGATTGGTTCACATATACAAGATGCTACTAAAATTCCATGCCAAAGTAATGAGTTTGTGAATGAACTTATTCGTGGTGTCAGGCTTCATTTTGACAAGTTTGTTGGTGATCTCAAGCCAGGAGATTTGGAGAAGGCGCAACTTGGTCTCTGCCACAGTTACAGCAGAGCAAAAGTGAAATTCAATGTTAATCGGGTTGACAATATGGTCATCCAAGCAATCTTCCTACTCGATACACTTGATAAGGATATCAATTCATTTGCCATGAGAGTCAGAGAATGGTATTCATGGCATTTTCCTGAACTAGTGAAGATCATAAATGATAATTATCTGTATTGCAAAGTAGCCAAATATGTTGAGGATAAGTCAAAGTTGGCTGAAGACAATATTGAAGCATTAACTGACTTAGTTGGAGACGAAGATAAAGCAAAGGAGATTGTTGAAGCAGCAAAAGCCTCCATGGGACAAGAATTGTCCCCTGTGGACTTGATCAATGTCCATCAGTTTGCTCAAAGGGTGATGGACCTATCTGACTACAGGAGAAGGTTGTCTGATTACCTGACTACTAAGATGAATGATATTGCACCTAATTTGCAATCTTTAGTGGGTGATAGTGTTGGAGCTCGTTTAATTTCCCATGCTGGTAGTCTCACAAATTTAGCTAAGTGCCCGTCATCAACTCTTCAGATTCTTGGTGCAGAGAAAGCTTTATTCAGGGCATTAAAAACAAGAGGAAATACTCCCAAATATGGTCTGATATTCCACTCTTCTTTTATTGGTCGAGCATCTGCCAAAAACAAGGGCCGCATGGCTCGCTATCTTGCAAACAAATGCTCTATTGCATCTAGGATTGACTGCTTTTCTGAAAAGGGTACTTCAGCATTTGGTGAGAAACTCCGTGAGCAAGTTGAGGAGCGACTTGACTTTTATGATAAGGGAGTTGCCCCTCGTAAGAACATTGATGTCATGAAATCTGCTATTGAAGTTGCCGACAACATAGATACAGAGATGGAGACAGAGGAAGCATCAGCCAAGAAAACCAAGAAGAAGAAGAAGCAAAAGGCTGGTGAAGATGAAGGAGAAGACTTAGCTGTTGAAAAATCATCAGAAATTACTAATGGTGATGCAGAGGATCATAAGTCAGAAAAGAAGAAGAAAAAGAAAGAAAAGAGAAAATTGGACCAGGAGGTAGATGTTCAAGATCAGATTGTTGATGAAGCTGCCAATGGCGAGTCTGGAAAGAAGAAGAAGAAGAAGTCAAAGAGGAAGGATGTCGATTAAAACTAAACTAGACCTTAAACTATGCTTGCTGTATTGGATGTTGTTATATTTTAGTTTTGTCTTTGAGTAACTTTNTAATTTNRTTGTTCTTAGTAACTTCTTTTATTTATTTNTTTTTTGTTTAGTTATCTATAGTACTTTAAATTTTTGTCGTAACTTTAGTTTAAAAAACGGT
TARGET=1389,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05619 Ps_contig_mira-and-tgicl-ass_franssen_38988
SEQUENCE=AAGCGAGTGGTATCGAACGCAGAGGTACGCGGGGCTACCACNATTCTCATTCACTTTTCACTTTCAAATCAAGTCTCTTCTTCTTCTTCTTCTTCAACAAATGGCGTTTTTCCGTTTCTCTCTTCTTTTCACTCTCTTCTTTTCCTCCATTCTCTTATTCAATCACTACGTCAAATGCGACAACGATGAGGAAGATGTGCTTTTTCAGGGAGTCAACAAGTACCGTGCATCATTAAACTTGACTTCTCTAACAAAGAATGACAATGCTAATTGTCTTGCTGAAGAATTAGCTGACCAGTTCAAGAGTCAACCTTGTACAAATACCACAGGTGCCAACACAGTGCCAGGTACTGAACCTAATTTCTCCAACTTTCCAGACCTTTTAACGAAATGCCACTTAAATATTTCTGACACAAGGGATGGAATGATAATGCCTGTTTGTGTTCCTGGCCTTGTTCCGAGCATTGTCCTCTCTAATTTCACACAATCTCTCTACTCACAAAGTCTCAATGACTCAAAGTATACTGGAATTGGTATTGGTTCAGAAGATAATTGGATCGTTGCTATACTGACTACAAACACTCCTGGCGGAAGCTTTGTTACCGGGACTTCTAATGGTGCCAATTTTATCAGCAAAATTGGACTGGTTTTCTCCTCAATCCTGTCGTTAGCTGTTAGCATTACCCTGTTTTGAGCAACAGGATACTGTTTGCTATTCTCATTTTATTCAGGACAGTCAGTAATGCTACGATATCGGTATGGGAGAAGTGCAAATAATGAATGCTATTGATTTTCAGTAAAGCATAGAGAGACTGTAGAATAATTGTTGCTTTGATATTGGCAACTGTATGTGAAATGCCTGAAAAGAAGAATAGGAGTATAGGACTACAGGAGGAAATAGAGTTTTTTTCACCAGGGTTGTTATCTTACTGTTATGGCTATCATAAACAAAATACTTTGTTTCACTGCTTTTAATTTTCTTAATTAGAACAGGAGCCAATAAATTGTTCCATTGATGGCAACTCAAGAGTATTTTAATTTTAAATTTGAAACTCTATTGCTTATTTGATGTTGAGATTGCCTAGTATGTAGTTCCAAGGTCATGTAATTCAGTTCCAAATCAGGATATTCTATTGTGTCACGGTATTACTGTTTAACT
TARGET=76,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0698
PRIMER_LEFT_PENALTY=1.052001
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=AGTGGTATCGAACGCAGAGGTA
PRIMER_RIGHT_SEQUENCE=TGGTCAGCTAATTCTTCAGCAA
PRIMER_LEFT=5,22
PRIMER_RIGHT=296,22
PRIMER_LEFT_TM=61.052
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=1.1250
PRIMER_LEFT_1_PENALTY=1.052001
PRIMER_RIGHT_1_PENALTY=0.073038
PRIMER_LEFT_1_SEQUENCE=AGTGGTATCGAACGCAGAGGTA
PRIMER_RIGHT_1_SEQUENCE=ATGATGCACGGTACTTGTTGAC
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=232,22
PRIMER_LEFT_1_TM=61.052
PRIMER_RIGHT_1_TM=59.927
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=1.1523
PRIMER_LEFT_2_PENALTY=1.134434
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=GGCTACCACNATTCTCATTCAC
PRIMER_RIGHT_2_SEQUENCE=TGGTCAGCTAATTCTTCAGCAA
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=296,22
PRIMER_LEFT_2_TM=58.866
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=1.1648
PRIMER_LEFT_3_PENALTY=1.146910
PRIMER_RIGHT_3_PENALTY=0.017846
PRIMER_LEFT_3_SEQUENCE=GTGGTATCGAACGCAGAGGTA
PRIMER_RIGHT_3_SEQUENCE=TGGTCAGCTAATTCTTCAGCAA
PRIMER_LEFT_3=6,21
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=60.147
PRIMER_RIGHT_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=1.2075
PRIMER_LEFT_4_PENALTY=1.134434
PRIMER_RIGHT_4_PENALTY=0.073038
PRIMER_LEFT_4_SEQUENCE=GGCTACCACNATTCTCATTCAC
PRIMER_RIGHT_4_SEQUENCE=ATGATGCACGGTACTTGTTGAC
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=232,22
PRIMER_LEFT_4_TM=58.866
PRIMER_RIGHT_4_TM=59.927
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=201
=
PRIMER_SEQUENCE_ID=SSR_05620 Ps_contig_mira-and-tgicl-ass_franssen_38989
SEQUENCE=GAAAGAAGTGAATGAATGAATGAATTGGAAGTGCATTGATTAGGGTTTGAGAATGGGTCGAAGCTTGTCACGTTCGCCACCTCCATACAGAAGAAGACACTCCCGATCTCGATCTCCGTCTCCATCTCATCGCCGAAACCGACGCCACCGTAGACACAGAAGCAGAACCTCTTCTTCACCCTCTCCTCCACCCAGATCACGCACTCCCTCTCTTAGACANNAAAAACACCATCCTCCAACCCTAAAACCAAACAAAAAGTAAACAAGGCTCCACTTGAGGAGGATTTGGAACTGTTGGAAGAAGATACTGCAAGAAGAATAGAAGAAGCTGTTCGAAGAAATGTGGAGGAGAGGCTTAAGTCTGAGGAAGTCAAGTTAGAAATAGAAAGGCGCATAGCAGAGGGATTGAAGAAGAGGTTTGATGATGTTGAAATTCAACTTACAAAAAAGAAAAGCAAGGAAATGCTTGCTGAAGCTAAGAAAGAAAAGNTGCAACAAGCTAACGAAAAGAGAGAGAA
TARGET=9,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05621 Ps_contig_mira-and-tgicl-ass_franssen_38998
SEQUENCE=CCTTTCCCAATTCTAGATCATACTCTCACTGTCTCCAACATTTCAATGATGATGCTGTYGATGCTGTTTCCTTGTTCAATCGATTGCTCCAAACGAAACCCACCCCACCCGCCATTGAATTTGGCAAGATTTTAGGTTCTCTTGTCAAAGCCAAACGTTATCCCACTGTTGTTTCCCTTTCTCAACAGATGGAATTGAGTAGAGTTACTCCTGATTTTGTAACTCACAACATCATCATCAATTCTCTCTCTCAATTAGGTCATATTACTTTTGCTTTTTCTGTTTTGGGGAAGATTCTCAAGAGGGGTTGTCTCCCAGATGCTATAACC
TARGET=243,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05622 Ps_contig_mira-and-tgicl-ass_franssen_39003
SEQUENCE=TCAGTGTTTGATGTTAGCATGACTTGGTGAATGTATGTGTGTTTTTTTTGTTTGTTTGCAAGTTTACATGGTTCTTATAGGTGTTGGTTTACATTGCAAGTTTTACTGCAAGTGTTCATGTACTGCATAACAAGTGTGTCCTTGGATTGATGTTGGACATTACTTTGGCTTGGCATATGGCTTGAACTTGGTCTACATCAGGGTTTATATTGTGGTTTTGCAAGTGTTTATGGCTTGCTGCTTGGTGTTTTGCAAAGCTTCATTTGGTTAGATCTAGTTGCAACAAGTCTGGCTTTGGCTCATGTTTCATTACATTGTTAACTGGTGGTATGGATTTGGTTAGGCTACATTGCAGCAAGGTTTTGTAGGTGTGCATTGTGACATGGTAAGCCTTGATATAGGTCATGAACACATGGTGTGGATTGTTGAACAACGGCTTGCAATATGATGTCATCAAGTTTGCACTTTGAAGTATCAATGACAAATGGCAACAATTAAGTAGGAAATTTGAAGATTAGAAATGGGATTTAGGATTTGGGGATGTAAAATAGTTGAGTTGACTTTGGTCAAAGTTGACCAAAAATTCAACGATTGACCAAAGTCAACATTTGGTCAATGTGTCATTTTTGTAGTATTTTTTCATTTATCGACACTTGTAATGAATATGTAATGAATGGAATTTGATATTACATGAAATTGACTTTGAATAAATCTTGTATGAACTTGGTATTTGAAATGAATGAATTTG
TARGET=47,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8268
PRIMER_LEFT_PENALTY=0.801088
PRIMER_RIGHT_PENALTY=0.025669
PRIMER_LEFT_SEQUENCE=TGATGTTAGCATGACTTGGTGA
PRIMER_RIGHT_SEQUENCE=TGCAGTACATGAACACTTGCAG
PRIMER_LEFT=8,22
PRIMER_RIGHT=126,22
PRIMER_LEFT_TM=59.199
PRIMER_RIGHT_TM=59.974
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=1.0209
PRIMER_LEFT_1_PENALTY=0.801088
PRIMER_RIGHT_1_PENALTY=0.219844
PRIMER_LEFT_1_SEQUENCE=TGATGTTAGCATGACTTGGTGA
PRIMER_RIGHT_1_SEQUENCE=CCATATGCCAAGCCAAAGTAAT
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=179,22
PRIMER_LEFT_1_TM=59.199
PRIMER_RIGHT_1_TM=60.220
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=1.0779
PRIMER_LEFT_2_PENALTY=0.801088
PRIMER_RIGHT_2_PENALTY=0.276812
PRIMER_LEFT_2_SEQUENCE=TGATGTTAGCATGACTTGGTGA
PRIMER_RIGHT_2_SEQUENCE=CAACATCAATCCAAGGACACAC
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=59.199
PRIMER_RIGHT_2_TM=60.277
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=1.2034
PRIMER_LEFT_3_PENALTY=0.801088
PRIMER_RIGHT_3_PENALTY=0.402305
PRIMER_LEFT_3_SEQUENCE=TGATGTTAGCATGACTTGGTGA
PRIMER_RIGHT_3_SEQUENCE=AGCCATAAACACTTGCAAAACC
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=234,22
PRIMER_LEFT_3_TM=59.199
PRIMER_RIGHT_3_TM=60.402
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=1.2110
PRIMER_LEFT_4_PENALTY=0.801088
PRIMER_RIGHT_4_PENALTY=0.409918
PRIMER_LEFT_4_SEQUENCE=TGATGTTAGCATGACTTGGTGA
PRIMER_RIGHT_4_SEQUENCE=CATCAATCCAAGGACACACTTG
PRIMER_LEFT_4=8,22
PRIMER_RIGHT_4=151,22
PRIMER_LEFT_4_TM=59.199
PRIMER_RIGHT_4_TM=60.410
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_05623 Ps_contig_mira-and-tgicl-ass_franssen_39012
SEQUENCE=TTAGGTTTAAGGTAACGTAGGTAAGTTGACGAGGGTGGACAACGAGGCCTCTCGTTCAGCGCCTCAACAAACCCATCTGGCAATGAATCAACTTCAGGAAGATGGTTTGCTTTAGGATTCTGCATTTTCCCTTGTTTGTTTGTTTTAGGTATGAAGTTGGGCAGGTATGAATGAGTGTGAGTGTTGAGAGTTTGTACAAAAAAAGTATGAATTCAAAGGATACATTATGAACACGAGTTCTCAACCAACAAAAGAAACATTGCAAAATGTGT
TARGET=132,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1862
PRIMER_LEFT_PENALTY=0.078029
PRIMER_RIGHT_PENALTY=0.108206
PRIMER_LEFT_SEQUENCE=GTAGGTAAGTTGACGAGGGTGG
PRIMER_RIGHT_SEQUENCE=ACTCATTCATACCTGCCCAACT
PRIMER_LEFT=16,22
PRIMER_RIGHT=175,22
PRIMER_LEFT_TM=59.922
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=0.2601
PRIMER_LEFT_1_PENALTY=0.078029
PRIMER_RIGHT_1_PENALTY=0.182028
PRIMER_LEFT_1_SEQUENCE=GTAGGTAAGTTGACGAGGGTGG
PRIMER_RIGHT_1_SEQUENCE=TTTTGTTGGTTGAGAACTCGTG
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=252,22
PRIMER_LEFT_1_TM=59.922
PRIMER_RIGHT_1_TM=60.182
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=0.3043
PRIMER_LEFT_2_PENALTY=0.122257
PRIMER_RIGHT_2_PENALTY=0.182028
PRIMER_LEFT_2_SEQUENCE=CAGGAAGATGGTTTGCTTTAGG
PRIMER_RIGHT_2_SEQUENCE=TTTTGTTGGTTGAGAACTCGTG
PRIMER_LEFT_2=94,22
PRIMER_RIGHT_2=252,22
PRIMER_LEFT_2_TM=60.122
PRIMER_RIGHT_2_TM=60.182
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=159
PRIMER_PAIR_PENALTY_3=0.3571
PRIMER_LEFT_3_PENALTY=0.248900
PRIMER_RIGHT_3_PENALTY=0.108206
PRIMER_LEFT_3_SEQUENCE=TCTGGCAATGAATCAACTTCAG
PRIMER_RIGHT_3_SEQUENCE=ACTCATTCATACCTGCCCAACT
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=175,22
PRIMER_LEFT_3_TM=60.249
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=0.4197
PRIMER_LEFT_4_PENALTY=0.237699
PRIMER_RIGHT_4_PENALTY=0.182028
PRIMER_LEFT_4_SEQUENCE=CTTCAGGAAGATGGTTTGCTTT
PRIMER_RIGHT_4_SEQUENCE=TTTTGTTGGTTGAGAACTCGTG
PRIMER_LEFT_4=91,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=59.762
PRIMER_RIGHT_4_TM=60.182
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_05624 Ps_contig_mira-and-tgicl-ass_franssen_39013
SEQUENCE=AAACACCACCANTAAGTAAAACGCGCTTGAAAACAATAAAGTTTTAACAAGAACCAACTCGAACTAAATGAACACAGAAACATATGCTTATAAGGTAGATGAGAATTGTCAATTGCTTTCAACTAATTAATTAAAAACAGATAAGGCGGTAGAAAGAAAATAGTTTGAAGGAAAAATAAAGCAACACAAAGACAGTCGCAGATCTTCCGGCTTCGGTTGGATAATTAAGAGGGCACAAAGAGTTATGAAGTGGACAAAGTCCACCTTTTCTAGTTGAAATCCATAGCAAAAAATAGCTTAAAATGCCCTATTTGTTGAGTATCTTAGATTCCTCGCTTCACAATCAGGGCCAGAGATCCAAAGAAATCTTATCAATGCTCAGGCTCAGTAGGTTTGACTTCATCAGCTTTTATGCTGTCCTCACCTCCATCCTCGGGCAAATCAGAGGTCCAGAGGGTCAGGTTGTCTCTCAACAACTGCATGATCAAAGTGCTGTCCTTGTATGACTCTTCACTCAAGGTATCCAACTCCGCAATCGCCTCATCAAAAGCTTGTTTGGCCAAATGGCAAGCCCTTTCAGGAGAGTTCATAATCTCATAATAAAAGACAGAGAAGTTGAGTGCAAGTCCAAGACGGATTGGATGTGTTGATGGAAGATCAGTGTTTGCAGTGGCTGAAGCAGCCTCATATCCTTTAAGTGACTGCTCAGCAGCCTCTTTCCTTTCTTGGTCAGTCTTGAACTCAGCAAGATACCGGAAATAATCACCTTTCATCTTATGATAGAAAACATTAGCTTCTGCCGAGGTGGAAGAAGGAACCAAGTGCTGATCTATAATAGTCAGAATGTCACCGCAAATTTTTGAGAGTTCCTCCTCGACCTTCTGGCAGTAGTTCTTGATCAATTTAACATTGTGTTCATTACCCTTCGACTCTTCCTTCTGCTCAATTGATGACATAATACGCCATGAGGCTCTTCGTGCACCAATGACATTTTTATATCCAACTGAGAGGAGGTTCCTCTCTTCCACAGTTAGTTCAACATCAAGTTTCGCAACTTTCTTCATACATTCAACCATTTCTTCATATCTCTCAGCCTGTTCAGAAAGCTTGGCCATGTAAACCTGGGTCTCTCTCTCTTTCTCGGCGGACATTGTGTCGTTTCAGAGAGAGATCGCAAAAAGTGAGAGAAAGCTGAAAGGGGGCGAGGAAGAGGAACAATAAGAAATGAGATGATGCGAGAGCCCNNGAC
TARGET=1127,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0414
PRIMER_LEFT_PENALTY=0.013714
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=ACATAATACGCCATGAGGCTCT
PRIMER_RIGHT_SEQUENCE=GCTTTCTCTCACTTTTTGCGAT
PRIMER_LEFT=952,22
PRIMER_RIGHT=1191,22
PRIMER_LEFT_TM=60.014
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.1484
PRIMER_LEFT_1_PENALTY=0.120688
PRIMER_RIGHT_1_PENALTY=0.027715
PRIMER_LEFT_1_SEQUENCE=TACCCTTCGACTCTTCCTTCTG
PRIMER_RIGHT_1_SEQUENCE=GCTTTCTCTCACTTTTTGCGAT
PRIMER_LEFT_1=919,22
PRIMER_RIGHT_1=1191,22
PRIMER_LEFT_1_TM=59.879
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.1573
PRIMER_LEFT_2_PENALTY=0.013714
PRIMER_RIGHT_2_PENALTY=0.143555
PRIMER_LEFT_2_SEQUENCE=ACATAATACGCCATGAGGCTCT
PRIMER_RIGHT_2_SEQUENCE=TCTTATTGTTCCTCTTCCTCGC
PRIMER_LEFT_2=952,22
PRIMER_RIGHT_2=1222,22
PRIMER_LEFT_2_TM=60.014
PRIMER_RIGHT_2_TM=59.856
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=0.1700
PRIMER_LEFT_3_PENALTY=0.013714
PRIMER_RIGHT_3_PENALTY=0.156287
PRIMER_LEFT_3_SEQUENCE=ACATAATACGCCATGAGGCTCT
PRIMER_RIGHT_3_SEQUENCE=TCTCTGAAACGACACAATGTCC
PRIMER_LEFT_3=952,22
PRIMER_RIGHT_3=1166,22
PRIMER_LEFT_3_TM=60.014
PRIMER_RIGHT_3_TM=60.156
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=215
PRIMER_PAIR_PENALTY_4=0.2770
PRIMER_LEFT_4_PENALTY=0.120688
PRIMER_RIGHT_4_PENALTY=0.156287
PRIMER_LEFT_4_SEQUENCE=TACCCTTCGACTCTTCCTTCTG
PRIMER_RIGHT_4_SEQUENCE=TCTCTGAAACGACACAATGTCC
PRIMER_LEFT_4=919,22
PRIMER_RIGHT_4=1166,22
PRIMER_LEFT_4_TM=59.879
PRIMER_RIGHT_4_TM=60.156
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_05625 Ps_contig_mira-and-tgicl-ass_franssen_39018
SEQUENCE=CCGTTCTTGATTTCAGATATTGAAACAGATGGTTCAACTCCCAAAATAAGATACATATCAAGAGGGATCTCCTTTCTGTCTTCTTCTTCTAGTTCGGAGAGCCGAATGCGATATTGTTTCAAATCATTGGTATAATTAATTGATCTATCAGATGTTCCTTTATGATTGGCATTATCCTCAAGCCCCTTACTGAGAAGAGAGATAAGTCTTCTAAGATCATTGGCTGCTTGAGAATAATCTCCGATCATCTCGTACAGACCTGCACGTCTAGAAAGTGCCTTCAAATAATTTCCATCAAGAGCTATAGCCAGACTGCAGTCTGCAATAGCATCGGTTAATTGACCTAACACTTTATATGTGGCGGCGCGATTACAATAACAGACAGCTGCAAATGGACGAGA
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2331
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=CAGATGGTTCAACTCCCAAAAT
PRIMER_RIGHT_SEQUENCE=TCTCTTCTCAGTAAGGGGCTTG
PRIMER_LEFT=25,22
PRIMER_RIGHT=199,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=175
PRIMER_PAIR_PENALTY_1=0.2331
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=CAGATGGTTCAACTCCCAAAAT
PRIMER_RIGHT_1_SEQUENCE=TCTTCTCAGTAAGGGGCTTGAG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.2394
PRIMER_LEFT_2_PENALTY=0.220992
PRIMER_RIGHT_2_PENALTY=0.018437
PRIMER_LEFT_2_SEQUENCE=CAGATGGTTCAACTCCCAAAAT
PRIMER_RIGHT_2_SEQUENCE=TCTCAAGCAGCCAATGATCTTA
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=232,22
PRIMER_LEFT_2_TM=60.221
PRIMER_RIGHT_2_TM=59.982
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=0.3097
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.088662
PRIMER_LEFT_3_SEQUENCE=CAGATGGTTCAACTCCCAAAAT
PRIMER_RIGHT_3_SEQUENCE=TCAGTAAGGGGCTTGAGGATAA
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=192,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=60.089
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=0.4570
PRIMER_LEFT_4_PENALTY=0.220992
PRIMER_RIGHT_4_PENALTY=0.235991
PRIMER_LEFT_4_SEQUENCE=CAGATGGTTCAACTCCCAAAAT
PRIMER_RIGHT_4_SEQUENCE=ATTCTCAAGCAGCCAATGATCT
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=234,22
PRIMER_LEFT_4_TM=60.221
PRIMER_RIGHT_4_TM=60.236
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_05626 Ps_contig_mira-and-tgicl-ass_franssen_39026
SEQUENCE=ACTTTTTCCTATTAAACCCCATTTAACCCTTATCCAATTTAAGTTCCAAATTAATCCATATTATCCATTTTTAACCAAAGTACACAGGCTGAGCTAAAAGTTAGTTGCTATGTGAAGCTGCAATTCAAAACAGCAGAACGAAACGCAAACTGCAATTCAAAACAAGCAGAACGAAACGCAAACTGCAATGCAAACAAGCCATATGTGAAAACAACGCCAGTAAGCCAACACTTCAAACGACAACACAACTTGCAAGCCGCAGCTCCAAATCTAAACTAAGCGCTTCCAAAAATCTTTTGAAAATTCGGTCGCGTTCACTCTTCGTTCTTTCACGCCGCTTCAATCACCGGATCTTAGTTGCATCGCTCTTCTTCACCAACGCGTCACATCGCTCATCTTCACCAATCGCGACGCCTCTCTCATCTTCACCAACTATATTGTTCATCCTCCTCAATCGGAAGCAGCTTCAGCTCGATCGAACAACAACAACAATAGAGTGCGACGATCTTGGATTTGACTCAGTATCGACGGCGTACGATCGAACAACCGAGTCGCCGCGCTTGAGGATGAAGTCGCCACGGTAATACAAAGTGAAGAATCCGAATCGAATTCCGCATTGGTTCGCGAGGTCACCGAGAGGATTTTTTTGACGCTGCTGAGTGAGAGAAAGAATCGCCGCGCCGGGATCGGAGTTTTGGCGTCCGTCGAACAGTCGTGGAGCCAGGAAGGTACGTGAGAGTGATGGCCACTAGTTCATTGTTTTCCTTCTGCGTATTTTCGTTGTTAGTTTGGCTTGCGAGGTGTGGTGTTGATTGTTTTGGGCCTGAGGCCCAACGCCGGTTTCGTCTYCACACCCCCTGGTGGACCGAATGCACCTCCCATTGTGTTTGGGCTTCTTCACCTCGGGCCCATGATGGCCTGTTAATTCCTGGCCCTAGTTTTTTGGAGGTTAGATGTAGGTTAACTTAATTGTTTTAAAGTTTAGATTTAGGATGGCTTAATGGATAATTTTAGACATTACTAGTTGATAATAATAATTAGAATGTTAATTGATTGT
TARGET=479,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05627 Ps_contig_mira-and-tgicl-ass_franssen_39033
SEQUENCE=TACAGACAAAAGGTAAGTAGCGACCCAAAAGTACATCAAAGTTGAGCAAGGATGAAGATGATGATCTTTGGGGTGCCATAGCAGCTCCTGCTCCAAAAACATCAAAACCTTTAAATTTGAAATCAGCTGCAACTGATGATGATGATCCCTGGGCTGCCATTGCGGCTCCTGCGCCCACTACAAGGGCCAAGCCTTTATCATCTGGCAGAGGCCGGGGAGCCAAACCTGCTGCTCCAAAATTAGGTGCTCRGCGGATAAACCGGACATCATCGTCCGGGATGTAAACCGAATTGCCACTTGTCAGGGACGAGGGAATGTTAGAATACAGTAACCCGTCTAGTATATCCGATAAGTTTTGTGCTTTCTAGTAGCATAATTGATTTGTAATATTTCTTTTTGATGTTTATTTTTGCTTTAGAAAGAAAATCTA
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05628 Ps_contig_mira-and-tgicl-ass_franssen_39034
SEQUENCE=CCTCAACACCTCTTTCAAGTCCACATTCAACACACCTCATCAGAATTGGCACGGCGCCACAATCCACCGCCCTTTTCCGGTTATCCCGAAAACAGCAAAGCGCGTAAAGTGCGGTAGCTGCCTCTTTCCTCTCTCTCCCCTTCCCATCCCGGAGAATCGCCACCAGAGCTCCAATTGCCCCTGGGAAAGCCCCAATAGTCGCTTTATTCACCTCCACAACCGCAAGGCTCGTGATTATTGTTGCGGCTAAAGCACGGCAGTCAGCCGAGGCTGCACGGAGGAGGAAAGCGACGACACGGGAGATTGCACCCTCGGCGACAAGTCCAATCTTAGTGTCGTCATCGAGGCTAAGGTTGAGAAGAAGAGAGAGAGCCTTCTCCTGAAGCTTAGAGTCCCCGGAGGAGGAGGATGAGTCGATGCAGAAGAGAACGGCGAAGACACG
TARGET=363,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5529
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=0.420643
PRIMER_LEFT_SEQUENCE=TCCAATCTTAGTGTCGTCATCG
PRIMER_RIGHT_SEQUENCE=TTCTCTTCTGCATCGACTCATC
PRIMER_LEFT=322,22
PRIMER_RIGHT=428,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=59.579
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.6000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=0.5567
PRIMER_LEFT_1_PENALTY=0.136083
PRIMER_RIGHT_1_PENALTY=0.420643
PRIMER_LEFT_1_SEQUENCE=CCCAATAGTCGCTTTATTCACC
PRIMER_RIGHT_1_SEQUENCE=TTCTCTTCTGCATCGACTCATC
PRIMER_LEFT_1=190,22
PRIMER_RIGHT_1=428,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=59.579
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.5567
PRIMER_LEFT_2_PENALTY=0.136083
PRIMER_RIGHT_2_PENALTY=0.420643
PRIMER_LEFT_2_SEQUENCE=CCCCAATAGTCGCTTTATTCAC
PRIMER_RIGHT_2_SEQUENCE=TTCTCTTCTGCATCGACTCATC
PRIMER_LEFT_2=189,22
PRIMER_RIGHT_2=428,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.579
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=0.7535
PRIMER_LEFT_3_PENALTY=0.332859
PRIMER_RIGHT_3_PENALTY=0.420643
PRIMER_LEFT_3_SEQUENCE=AAGCCCCAATAGTCGCTTTATT
PRIMER_RIGHT_3_SEQUENCE=TTCTCTTCTGCATCGACTCATC
PRIMER_LEFT_3=186,22
PRIMER_RIGHT_3=428,22
PRIMER_LEFT_3_TM=60.333
PRIMER_RIGHT_3_TM=59.579
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.9191
PRIMER_LEFT_4_PENALTY=0.043085
PRIMER_RIGHT_4_PENALTY=0.875979
PRIMER_LEFT_4_SEQUENCE=CTCTTTCCTCTCTCTCCCCTTC
PRIMER_RIGHT_4_SEQUENCE=GGGACTCTAAGCTTCAGGAGAA
PRIMER_LEFT_4=121,22
PRIMER_RIGHT_4=395,22
PRIMER_LEFT_4_TM=59.957
PRIMER_RIGHT_4_TM=59.124
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=0.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_05629 Ps_contig_mira-and-tgicl-ass_franssen_39034
SEQUENCE=CCTCAACACCTCTTTCAAGTCCACATTCAACACACCTCATCAGAATTGGCACGGCGCCACAATCCACCGCCCTTTTCCGGTTATCCCGAAAACAGCAAAGCGCGTAAAGTGCGGTAGCTGCCTCTTTCCTCTCTCTCCCCTTCCCATCCCGGAGAATCGCCACCAGAGCTCCAATTGCCCCTGGGAAAGCCCCAATAGTCGCTTTATTCACCTCCACAACCGCAAGGCTCGTGATTATTGTTGCGGCTAAAGCACGGCAGTCAGCCGAGGCTGCACGGAGGAGGAAAGCGACGACACGGGAGATTGCACCCTCGGCGACAAGTCCAATCTTAGTGTCGTCATCGAGGCTAAGGTTGAGAAGAAGAGAGAGAGCCTTCTCCTGAAGCTTAGAGTCCCCGGAGGAGGAGGATGAGTCGATGCAGAAGAGAACGGCGAAGACACG
TARGET=398,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2812
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=1.148923
PRIMER_LEFT_SEQUENCE=TCCAATCTTAGTGTCGTCATCG
PRIMER_RIGHT_SEQUENCE=CGTTCTCTTCTGCATCGACTC
PRIMER_LEFT=322,22
PRIMER_RIGHT=430,21
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=60.149
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.6000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=1.2850
PRIMER_LEFT_1_PENALTY=0.136083
PRIMER_RIGHT_1_PENALTY=1.148923
PRIMER_LEFT_1_SEQUENCE=CCCAATAGTCGCTTTATTCACC
PRIMER_RIGHT_1_SEQUENCE=CGTTCTCTTCTGCATCGACTC
PRIMER_LEFT_1=190,22
PRIMER_RIGHT_1=430,21
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=1.2850
PRIMER_LEFT_2_PENALTY=0.136083
PRIMER_RIGHT_2_PENALTY=1.148923
PRIMER_LEFT_2_SEQUENCE=CCCCAATAGTCGCTTTATTCAC
PRIMER_RIGHT_2_SEQUENCE=CGTTCTCTTCTGCATCGACTC
PRIMER_LEFT_2=189,22
PRIMER_RIGHT_2=430,21
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=1.4818
PRIMER_LEFT_3_PENALTY=0.332859
PRIMER_RIGHT_3_PENALTY=1.148923
PRIMER_LEFT_3_SEQUENCE=AAGCCCCAATAGTCGCTTTATT
PRIMER_RIGHT_3_SEQUENCE=CGTTCTCTTCTGCATCGACTC
PRIMER_LEFT_3=186,22
PRIMER_RIGHT_3=430,21
PRIMER_LEFT_3_TM=60.333
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=1.6094
PRIMER_LEFT_4_PENALTY=0.460467
PRIMER_RIGHT_4_PENALTY=1.148923
PRIMER_LEFT_4_SEQUENCE=TAGTGTCGTCATCGAGGCTAAG
PRIMER_RIGHT_4_SEQUENCE=CGTTCTCTTCTGCATCGACTC
PRIMER_LEFT_4=330,22
PRIMER_RIGHT_4=430,21
PRIMER_LEFT_4_TM=59.540
PRIMER_RIGHT_4_TM=60.149
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_05630 Ps_contig_mira-and-tgicl-ass_franssen_39039
SEQUENCE=ATAATAAAATAAAAGAAAAATAACAAGTAGNAAAGAAGTTCACGTCATCTTCTTCTTCTGTCACCGGACGGAAACTTCACTACCACCAAAACTCAACCAATATGTAAATTGGAGGTACTGTTCCATTCGGATTTCCATCCTCAACCTAGCTCTAATCCTTGAACTCTCATAATCTTCGCCAATCCTATTTAATTTCATATTTTGTTCAAGAACCCTAAATGCTTCAACTTAAATTAAACACTAATGGATTATTGAATTTAGCAATAGTCATTGGGGAATGAATCATATTAACATGAAGAACAAGATTGTATGAAGAACTTGGTGGAAAGAGAGATGGAGACCCTACCAATAGGGACGTGATTTTCCCCGGTAATTG
TARGET=48,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05631 Ps_contig_mira-and-tgicl-ass_franssen_39041
SEQUENCE=TTCTAACCACCATTTAACTTATTTTTGCAAGATATAGAAACATAACAGAAAATAAAATTAGAAGCAAAACTGAGATGTCATCTATAAAGAAAAGAAAACTCGACAACAATTGTCTTACCCGCCACCATTTTATCCCCCAACATGTCAATTGCAGTAAAAGCAAAGCAAACGGGGATAAACAATGGAACAAAGAACCTAAATTTACACTTTAAACCATCAAATAAAATTTACAGGAAATAAATTAGCATAGTTCTTTCCTGCTTTAAGTTCACTACCAAAATTGAAAATAAAAGTAATATGCGAAAAGTGTTCACTTGGCACCCAAGGTTGGAAAATGACCAGGATCTTCAATGGATGGGGCGGGTACATTGCTAGTTGAATTTCCACGATAACCTCCAAAACCTCCTCTTGTACCACGGCCGCCGCGTCCTCTAGCACGTCCACCTGGGTTGTAATAACCTTCACCTTCAGCTGGCTTCAAAAACTCATTGATGCTGACAGACTTTTTGGCCTTCTCCTCCTTGTCAAAAGCTTCTTTGCGCTTATCCTTATCAGAGCCCAATTTAGCAAAGATTTCAAAATTGTCTTTCTTGCACGATAGGGCTTGCATAGTTTCAAACTCTTTCTTGTCAACCTTTCTTTCTTCAGTCTTGAGTGCTTGCAAAGCCTTTCTTTTCTCTTCCAGCAGTTTCTCATATTCCTCCAGAGTCATTTCCTTATCCTCCGGCTCTTTTTCTTCAGCTTCATTAGCAGGATTATCCTTGTTACCGTCAGCAACATCATTTTCAGCAGCAGGCTTCTCATCACCCAAATTCTTTTCAGCTTCGTTCACAACTTCATCAGTCACCTGGGTAACTTCATCAGTTTCTGTCCCCCAGTTACCGCGTCCAGAACCTTCACGTTTGAATTCGCTTCCTCTCCCAGTGCCACTATGGCGTTCAAATGTCCTTCTAGGTCTCCCTTCTTCACCACCTTCACCATTGCTGAAACCTCCACGACGACCTCCACGATAGGGAGGTCGAGGTCCACCAAAGCCACGTCTCTCAGAGAACTTATCTCCTTCAAGAGCACCCTGACTATCAGGAGCTCTGGAGACAGTGAATGAGTTCTCGTCGTTTGAGAAGTCACGGCCAAAACCACGACCACGGCCACCGCCACGTCCACCAACAAATCCACGTCCACCACGGCCTCCGCGACCTCCTTCATTCCTGGATTCCCTCACAGCTTGAGCGGGTGGAGCCGGCTTAGAAGGCAGCTGAGCCGGCTTATTCTGCTGAGTGCGCTTTCCCTGGTCCTTCTCGGCAGGTTTCTTCAGAGGAGCCGCAGCCGCTTTGAGCTGTTCGGTCAGGATGAGCTGAGAAGGATCCTCAACGTCATCACCTAGCAAATCAAATGGATTCGCAGTGGCCATCTTATTCGTAAAGACAACAGCAGCAACAAAAACAAAAGACGAGAATCGGTTTCACTTCACACGGTAACTGTAACACTATGCTTGCAATGTTTCCATAGTACTACAAAGTAAGGGTTTAGATCGGAGAGAGAAGAAGAAGACAAGGGTTTGAGAAACCGTGAAACTGAAAACCCTAGCCGCAAAGGAGAGAGAAAGAGAAGAGAGTGTGTTGAAGTCCCCGCGTACTAGT
TARGET=1540,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1455
PRIMER_LEFT_PENALTY=0.052366
PRIMER_RIGHT_PENALTY=0.093130
PRIMER_LEFT_SEQUENCE=ATTCGTAAAGACAACAGCAGCA
PRIMER_RIGHT_SEQUENCE=TAGGGTTTTCAGTTTCACGGTT
PRIMER_LEFT=1414,22
PRIMER_RIGHT=1585,22
PRIMER_LEFT_TM=59.948
PRIMER_RIGHT_TM=59.907
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.1742
PRIMER_LEFT_1_PENALTY=0.081111
PRIMER_RIGHT_1_PENALTY=0.093130
PRIMER_LEFT_1_SEQUENCE=GAGAAGGATCCTCAACGTCATC
PRIMER_RIGHT_1_SEQUENCE=TAGGGTTTTCAGTTTCACGGTT
PRIMER_LEFT_1=1356,22
PRIMER_RIGHT_1=1585,22
PRIMER_LEFT_1_TM=60.081
PRIMER_RIGHT_1_TM=59.907
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=230
PRIMER_PAIR_PENALTY_2=0.2468
PRIMER_LEFT_2_PENALTY=0.153708
PRIMER_RIGHT_2_PENALTY=0.093130
PRIMER_LEFT_2_SEQUENCE=GTCAGGATGAGCTGAGAAGGAT
PRIMER_RIGHT_2_SEQUENCE=TAGGGTTTTCAGTTTCACGGTT
PRIMER_LEFT_2=1343,22
PRIMER_RIGHT_2=1585,22
PRIMER_LEFT_2_TM=59.846
PRIMER_RIGHT_2_TM=59.907
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=243
PRIMER_PAIR_PENALTY_3=0.3068
PRIMER_LEFT_3_PENALTY=0.052366
PRIMER_RIGHT_3_PENALTY=0.254467
PRIMER_LEFT_3_SEQUENCE=ATTCGTAAAGACAACAGCAGCA
PRIMER_RIGHT_3_SEQUENCE=TTCTCTTTCTCTCTCCTTTGCG
PRIMER_LEFT_3=1414,22
PRIMER_RIGHT_3=1609,22
PRIMER_LEFT_3_TM=59.948
PRIMER_RIGHT_3_TM=60.254
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=10.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=0.3356
PRIMER_LEFT_4_PENALTY=0.081111
PRIMER_RIGHT_4_PENALTY=0.254467
PRIMER_LEFT_4_SEQUENCE=GAGAAGGATCCTCAACGTCATC
PRIMER_RIGHT_4_SEQUENCE=TTCTCTTTCTCTCTCCTTTGCG
PRIMER_LEFT_4=1356,22
PRIMER_RIGHT_4=1609,22
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=60.254
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=10.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_05632 Ps_contig_mira-and-tgicl-ass_franssen_39043
SEQUENCE=ATAGTGAAACTTTTAAATAGCATAAAGNATAGTCAATGGGGNGGTTTGAGGCTGACGATGGATTTAGCATATGCTTCCCCTTTGTTCCCTTTCCTTGTGCTAGTAACTTTCGTTTCTATGCTATCATCATCATCATCAACCACCTTGGCCAGTGGATTCTCAATCAAGGAAGCAACTGTGCAAGATCTCCAACTAGCTTTCCAAACAAACCAACTAACATCAAGACAACTTGTTGAGTTCTACCTCAACCAAATCAAAATCCAAAACCCACTTCTCAAAGGAGTGTTGGAAGTGAATCCAGATGCACTAACACAAGCAGATGCAGCTGACAAAGAGAGAAGGGAAAAGGCACCAAGTTCTCTATCAAGGTTGCATGGGATACCTATTTTGGTTAAGGATAATATTGCAACTAAGGATAAACTGAACACTACTGCAGGCTCTTATGCACTACTTGGATCAGTGGTGCCAAGAGATGCAGGTGTAGTTACCAAGTTAAGAAAAGCTGGGGCTATCATTTTAGGGAAAGCCACATTGAGTGAGTGGGCGCATTACAGGGATTCTGATATTCCTAGTGGTTGGAGTGCCAGAGGTGGACAAGGAAAGAATCCATATACATTGGGTGAACCCTGTGGTTCAAGTAGTGGATCAGCAATATCAGTAGCTGCAAATTTGGTGACTGTATCGCTTGGCACCGAAACCGATGGCTCCATTTTATGCCCTTCAAGTATGAACTCAGTAGTAGGCATCAAACCCACAGTTGGTCTCACTAGTAGAGCGGGTGTAGTTCCAATTAGTCCAAGGCAAGACACTG
TARGET=123,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1669
PRIMER_LEFT_PENALTY=0.164560
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=TGTTCCCTTTCCTTGTGCTAGT
PRIMER_RIGHT_SEQUENCE=TTTCCCTTCTCTCTTTGTCAGC
PRIMER_LEFT=82,22
PRIMER_RIGHT=345,22
PRIMER_LEFT_TM=60.165
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.2949
PRIMER_LEFT_1_PENALTY=0.164560
PRIMER_RIGHT_1_PENALTY=0.130358
PRIMER_LEFT_1_SEQUENCE=TGTTCCCTTTCCTTGTGCTAGT
PRIMER_RIGHT_1_SEQUENCE=CCTTGATAGAGAACTTGGTGCC
PRIMER_LEFT_1=82,22
PRIMER_RIGHT_1=368,22
PRIMER_LEFT_1_TM=60.165
PRIMER_RIGHT_1_TM=60.130
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.3014
PRIMER_LEFT_2_PENALTY=0.164560
PRIMER_RIGHT_2_PENALTY=0.136881
PRIMER_LEFT_2_SEQUENCE=TGTTCCCTTTCCTTGTGCTAGT
PRIMER_RIGHT_2_SEQUENCE=CTCCTTTGAGAAGTGGGTTTTG
PRIMER_LEFT_2=82,22
PRIMER_RIGHT_2=282,22
PRIMER_LEFT_2_TM=60.165
PRIMER_RIGHT_2_TM=60.137
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.3141
PRIMER_LEFT_3_PENALTY=0.164560
PRIMER_RIGHT_3_PENALTY=0.149516
PRIMER_LEFT_3_SEQUENCE=TGTTCCCTTTCCTTGTGCTAGT
PRIMER_RIGHT_3_SEQUENCE=GAGAAGTGGGTTTTGGATTTTG
PRIMER_LEFT_3=82,22
PRIMER_RIGHT_3=275,22
PRIMER_LEFT_3_TM=60.165
PRIMER_RIGHT_3_TM=59.850
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=0.3994
PRIMER_LEFT_4_PENALTY=0.164560
PRIMER_RIGHT_4_PENALTY=0.234845
PRIMER_LEFT_4_SEQUENCE=TGTTCCCTTTCCTTGTGCTAGT
PRIMER_RIGHT_4_SEQUENCE=GCCTTTTCCCTTCTCTCTTTGT
PRIMER_LEFT_4=82,22
PRIMER_RIGHT_4=349,22
PRIMER_LEFT_4_TM=60.165
PRIMER_RIGHT_4_TM=60.235
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_05633 Ps_contig_mira-and-tgicl-ass_franssen_39046
SEQUENCE=ACGGTCAAGCTTTTGCTTTTACTCAAAATCATGACAACATTTCTACATTTATAAAAGTAAAACATAAAACACAAAGGTAAAAATTAAAACAATCTTTCAAAGACCAAGATAATAATTTAAACTCAACTAACCAATACAACAAGGGAGAGGCATCGAAGGAAATGATATCATCATCTTTAATACTATCTTAAACTATGAACATAAACAATGCCATAACAAATTATGATGTCTCTCAGCAGCACAATATTAACACCAAAATGAACATGATAATTCCATGTCATAGAACAACCCTGTAAAGTGAGAGTGGTACACACTCAACAAAAGATATATTTCTTTCAAACTTTGAGTCTCAACACCATTGACCATAAAAATAATAATTAGAAGTCAACCTGCCAATGCCATGTGCCATGGCTTTATATTACATCCATGAAGCGATTACATGATGGCGCAGCGGCTCTTTTCTTCAGTTGCCTGTGAGTTTACTTGCTGGAACCTTTGCGACTCGGATGGGTTGTATGTAAACTCTGAAGTATCAAGTCCATACTTCTCCCTCATCCGTGAACTCCAAGCATCATTTCTGTTAGGGCGCTGGTCAATTGCCCCAGCAACTGGTACCCCTGCTGCTGGACCTGCTTGTCTATTGAGCAATGGCTGCCGGGCTTGCTGCCTTGGATTAATGAGCTCATCATCACTATCATAATCTGCTGGCCTATTTACAGCCCTAACAATAAGGGCTAACAAGAAAAGAAGAGCCTCAAAGATGACAATCCCAAGAGCAGCCCATCTCACAATACTCCAATTCTCTCTCAGAAAGCCATATATCATATCAAAATCTCCACTCTTATCTGTTGGGATTACTTCTTTCCAGCTTTTGTCAAAAAACAGAAAGGCTGCACATCCCAGCTCTACCAAGATCAACAGTGCCACCAAAATTGAATAACAAATCAGGCAGCACCCGTTCCCTGTCATAGCTCCAATACAGCCAAAACAAGATACAACGAAGAGAACAACTCCAACACCAATAAATAAGTAAATAAACCAAGCCTTAGGCAACTTGTCAAAAATATTATCGGATAGAGACACAGCCATAAGCATCGGCCGTCCAAGTTGAATCAGAGCACTATCATCACTTGCTGGAGAAATCGCCGGCGTATCATCATCAGAAGCTTTACTGAATTGAACCACCAAATAGATCCCATAACCCACCATGGCCAAACCAGTCACACTCAGAACGAAGTTGAAAAGCTTCAAAAGACACTCCCAGAATCCCCTACACGCCATCCTCCTTTACCTGTAAATAACTGTGATTTAATCCCTAAACCAGAATTTAAAACGGAGTTCAATTCCCTCTTTATATGTCTTAGAAGAGAAATCGAATCGATGATTATGGAGTTGGTGAATTGCGAAGCTTCGTTGTTTCGCTGTACAAAATTCAAATAAAAGTAGGGAAGAAGAAGAAGAAATGGTAAAGTGTGTGGTTTGTGCCACTCTG
TARGET=1448,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9551
PRIMER_LEFT_PENALTY=0.078029
PRIMER_RIGHT_PENALTY=0.877067
PRIMER_LEFT_SEQUENCE=AAGCTTCAAAAGACACTCCCAG
PRIMER_RIGHT_SEQUENCE=GCACAAACCACACACTTTACCA
PRIMER_LEFT=1242,22
PRIMER_RIGHT=1484,22
PRIMER_LEFT_TM=59.922
PRIMER_RIGHT_TM=60.877
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.9551
PRIMER_LEFT_1_PENALTY=0.078029
PRIMER_RIGHT_1_PENALTY=0.877067
PRIMER_LEFT_1_SEQUENCE=AAGCTTCAAAAGACACTCCCAG
PRIMER_RIGHT_1_SEQUENCE=TGGCACAAACCACACACTTTAC
PRIMER_LEFT_1=1242,22
PRIMER_RIGHT_1=1486,22
PRIMER_LEFT_1_TM=59.922
PRIMER_RIGHT_1_TM=60.877
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.9551
PRIMER_LEFT_2_PENALTY=0.078029
PRIMER_RIGHT_2_PENALTY=0.877067
PRIMER_LEFT_2_SEQUENCE=AAGCTTCAAAAGACACTCCCAG
PRIMER_RIGHT_2_SEQUENCE=GTGGCACAAACCACACACTTTA
PRIMER_LEFT_2=1242,22
PRIMER_RIGHT_2=1487,22
PRIMER_LEFT_2_TM=59.922
PRIMER_RIGHT_2_TM=60.877
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=1.0541
PRIMER_LEFT_3_PENALTY=0.177008
PRIMER_RIGHT_3_PENALTY=0.877067
PRIMER_LEFT_3_SEQUENCE=CGATGATTATGGAGTTGGTGAA
PRIMER_RIGHT_3_SEQUENCE=TGGCACAAACCACACACTTTAC
PRIMER_LEFT_3=1378,22
PRIMER_RIGHT_3=1486,22
PRIMER_LEFT_3_TM=59.823
PRIMER_RIGHT_3_TM=60.877
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=1.0541
PRIMER_LEFT_4_PENALTY=0.177008
PRIMER_RIGHT_4_PENALTY=0.877067
PRIMER_LEFT_4_SEQUENCE=CGATGATTATGGAGTTGGTGAA
PRIMER_RIGHT_4_SEQUENCE=GTGGCACAAACCACACACTTTA
PRIMER_LEFT_4=1378,22
PRIMER_RIGHT_4=1487,22
PRIMER_LEFT_4_TM=59.823
PRIMER_RIGHT_4_TM=60.877
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_05634 Ps_contig_mira-and-tgicl-ass_franssen_39047
SEQUENCE=CTTACTATAACTCTACTAANAAAGTTTTTATTTTTTCTTTTCTTTCTNTACTNACTAAACTAACAAATAGATAAAATTACTAAAGAACAATTAAACTACTATTGTAACAGAAATTTAGACATAAGTCACAGCAGCAGAATCAGCGGCGGACAGACGGAACGGACGGACGGAGGCAACTCAGAGATGCAAATCGGTGGCGCGGATCTCTTCATCATTGTAGTTCATCGGAGCAGGCAAATTGAGATCAATATTGCGAGGCTGAGGCTTCCGAACAGGGGAGTAAATGATGACACAATCTTCATCATCATCCACCACAGATGAAGAAGAATCACAATCACTGTGATAACCCTCAGCAGCAACAATCGGAACAAACACAGGAACCCTAGAGGTTGAAGAAGAACCAACCCTAGGTCCACTGAACGATTCCACGGTACTACTCATCCCGCTCGAAGTAGGATAATTGACATAAACCGTGTCCGCTTTGGTAAGCACGGCTGGTTTAGGCCTACGGTTAACCGGAGGTGGAGGAGGAGGAATCAGAGCCGCAAGAGCCTCAGGAGGGATAGGGAAATTGGTCTTGGCTTTGGCACCGCGGAAGGATATTGCAGCTGCGTCGTAAGCCCGGGCGGCTTGAATTGCAGAATCAAAAGTGCCTAACCAGACCCGGGCTTTCTTCCACGGATCTCTTATCTCCGCCGCGTATCGGCCCCATGGTCGCTTCCGAACACCGCGGTAACGGATTTCCGGTGCCGCAGGAGGATTCGGTCCGGCAACCGGGTTTCGATCTACGCGGGGTGCGCCTTTCACTCGAGCCATCGATGCTGTCACAGAAAAAACTAAACGNGTAAAGAAAGAGAGAGAAAGTTTAGTTTTGGGGACCCATATGGTCTTGCTTCT
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4862
PRIMER_LEFT_PENALTY=1.455083
PRIMER_RIGHT_PENALTY=0.031122
PRIMER_LEFT_SEQUENCE=CATAAGTCACAGCAGCAGAATCA
PRIMER_RIGHT_SEQUENCE=TCAACCTCTAGGGTTCCTGTGT
PRIMER_LEFT=119,23
PRIMER_RIGHT=392,22
PRIMER_LEFT_TM=60.455
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=1.5544
PRIMER_LEFT_1_PENALTY=1.455083
PRIMER_RIGHT_1_PENALTY=0.099308
PRIMER_LEFT_1_SEQUENCE=CATAAGTCACAGCAGCAGAATCA
PRIMER_RIGHT_1_SEQUENCE=CTAGGGTTCCTGTGTTTGTTCC
PRIMER_LEFT_1=119,23
PRIMER_RIGHT_1=385,22
PRIMER_LEFT_1_TM=60.455
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=1.5662
PRIMER_LEFT_2_PENALTY=1.455083
PRIMER_RIGHT_2_PENALTY=0.111077
PRIMER_LEFT_2_SEQUENCE=CATAAGTCACAGCAGCAGAATCA
PRIMER_RIGHT_2_SEQUENCE=CTTCTTCATCTGTGGTGGATGA
PRIMER_LEFT_2=119,23
PRIMER_RIGHT_2=325,22
PRIMER_LEFT_2_TM=60.455
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=207
PRIMER_PAIR_PENALTY_3=1.6024
PRIMER_LEFT_3_PENALTY=1.455083
PRIMER_RIGHT_3_PENALTY=0.147295
PRIMER_LEFT_3_SEQUENCE=CATAAGTCACAGCAGCAGAATCA
PRIMER_RIGHT_3_SEQUENCE=TGTGTCATCATTTACTCCCCTG
PRIMER_LEFT_3=119,23
PRIMER_RIGHT_3=293,22
PRIMER_LEFT_3_TM=60.455
PRIMER_RIGHT_3_TM=59.853
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=1.6585
PRIMER_LEFT_4_PENALTY=1.455083
PRIMER_RIGHT_4_PENALTY=0.203457
PRIMER_LEFT_4_SEQUENCE=CATAAGTCACAGCAGCAGAATCA
PRIMER_RIGHT_4_SEQUENCE=TGTGGTGGATGATGATGAAGAT
PRIMER_LEFT_4=119,23
PRIMER_RIGHT_4=315,22
PRIMER_LEFT_4_TM=60.455
PRIMER_RIGHT_4_TM=60.203
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=197
=
PRIMER_SEQUENCE_ID=SSR_05635 Ps_contig_mira-and-tgicl-ass_franssen_39047
SEQUENCE=CTTACTATAACTCTACTAANAAAGTTTTTATTTTTTCTTTTCTTTCTNTACTNACTAAACTAACAAATAGATAAAATTACTAAAGAACAATTAAACTACTATTGTAACAGAAATTTAGACATAAGTCACAGCAGCAGAATCAGCGGCGGACAGACGGAACGGACGGACGGAGGCAACTCAGAGATGCAAATCGGTGGCGCGGATCTCTTCATCATTGTAGTTCATCGGAGCAGGCAAATTGAGATCAATATTGCGAGGCTGAGGCTTCCGAACAGGGGAGTAAATGATGACACAATCTTCATCATCATCCACCACAGATGAAGAAGAATCACAATCACTGTGATAACCCTCAGCAGCAACAATCGGAACAAACACAGGAACCCTAGAGGTTGAAGAAGAACCAACCCTAGGTCCACTGAACGATTCCACGGTACTACTCATCCCGCTCGAAGTAGGATAATTGACATAAACCGTGTCCGCTTTGGTAAGCACGGCTGGTTTAGGCCTACGGTTAACCGGAGGTGGAGGAGGAGGAATCAGAGCCGCAAGAGCCTCAGGAGGGATAGGGAAATTGGTCTTGGCTTTGGCACCGCGGAAGGATATTGCAGCTGCGTCGTAAGCCCGGGCGGCTTGAATTGCAGAATCAAAAGTGCCTAACCAGACCCGGGCTTTCTTCCACGGATCTCTTATCTCCGCCGCGTATCGGCCCCATGGTCGCTTCCGAACACCGCGGTAACGGATTTCCGGTGCCGCAGGAGGATTCGGTCCGGCAACCGGGTTTCGATCTACGCGGGGTGCGCCTTTCACTCGAGCCATCGATGCTGTCACAGAAAAAACTAAACGNGTAAAGAAAGAGAGAGAAAGTTTAGTTTTGGGGACCCATATGGTCTTGCTTCT
TARGET=524,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2618
PRIMER_LEFT_PENALTY=0.125755
PRIMER_RIGHT_PENALTY=0.135999
PRIMER_LEFT_SEQUENCE=AAGAAGAACCAACCCTAGGTCC
PRIMER_RIGHT_SEQUENCE=GGTTAGGCACTTTTGATTCTGC
PRIMER_LEFT=392,22
PRIMER_RIGHT=658,22
PRIMER_LEFT_TM=59.874
PRIMER_RIGHT_TM=60.136
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.2693
PRIMER_LEFT_1_PENALTY=0.125755
PRIMER_RIGHT_1_PENALTY=0.143555
PRIMER_LEFT_1_SEQUENCE=AAGAAGAACCAACCCTAGGTCC
PRIMER_RIGHT_1_SEQUENCE=TAAGAGATCCGTGGAAGAAAGC
PRIMER_LEFT_1=392,22
PRIMER_RIGHT_1=688,22
PRIMER_LEFT_1_TM=59.874
PRIMER_RIGHT_1_TM=59.856
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.3101
PRIMER_LEFT_2_PENALTY=0.111077
PRIMER_RIGHT_2_PENALTY=0.199067
PRIMER_LEFT_2_SEQUENCE=CATCCACCACAGATGAAGAAGA
PRIMER_RIGHT_2_SEQUENCE=AAGCCAAGACCAATTTCCCTAT
PRIMER_LEFT_2=305,22
PRIMER_RIGHT_2=583,22
PRIMER_LEFT_2_TM=60.111
PRIMER_RIGHT_2_TM=60.199
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.3101
PRIMER_LEFT_3_PENALTY=0.111077
PRIMER_RIGHT_3_PENALTY=0.199067
PRIMER_LEFT_3_SEQUENCE=TCATCCACCACAGATGAAGAAG
PRIMER_RIGHT_3_SEQUENCE=AAGCCAAGACCAATTTCCCTAT
PRIMER_LEFT_3=304,22
PRIMER_RIGHT_3=583,22
PRIMER_LEFT_3_TM=60.111
PRIMER_RIGHT_3_TM=60.199
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.3248
PRIMER_LEFT_4_PENALTY=0.125755
PRIMER_RIGHT_4_PENALTY=0.199067
PRIMER_LEFT_4_SEQUENCE=AAGAAGAACCAACCCTAGGTCC
PRIMER_RIGHT_4_SEQUENCE=AAGCCAAGACCAATTTCCCTAT
PRIMER_LEFT_4=392,22
PRIMER_RIGHT_4=583,22
PRIMER_LEFT_4_TM=59.874
PRIMER_RIGHT_4_TM=60.199
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=7.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_05636 Ps_contig_mira-and-tgicl-ass_franssen_39059
SEQUENCE=TTCTACAANAAATTCAAAAATTAACCTAAGACTCCTTTACTACACTGTTCCTTCGCCAAGCCAAGCCAACACCATCAACTACCTCCGCCNGAAAAACAAAAACAAATAAAAATAGCTCATCGAATGCAACCTTGTGATTCTCTTGTCAAGAGAAAATTCAACCTTTCCAAGTAACCCGAAGCATTGAGCAGCTTAGTTTCTCCAAACTTATATGTCATAGACCGCAAGAACGGGTCTTCAAAACTCAAAGCGTTGAAGCTGCATAACAGCATAAATAAAATAAAGCGAGGAG
TARGET=55,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.4781
PRIMER_LEFT_PENALTY=3.355446
PRIMER_RIGHT_PENALTY=0.122657
PRIMER_LEFT_SEQUENCE=TCCTTTACTACACTGTTCCTTCG
PRIMER_RIGHT_SEQUENCE=AGGTTGCATTCGATGAGCTATT
PRIMER_LEFT=32,23
PRIMER_RIGHT=131,22
PRIMER_LEFT_TM=57.645
PRIMER_RIGHT_TM=60.123
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=3.5324
PRIMER_LEFT_1_PENALTY=3.409754
PRIMER_RIGHT_1_PENALTY=0.122657
PRIMER_LEFT_1_SEQUENCE=CTCCTTTACTACACTGTTCCTTCG
PRIMER_RIGHT_1_SEQUENCE=AGGTTGCATTCGATGAGCTATT
PRIMER_LEFT_1=31,24
PRIMER_RIGHT_1=131,22
PRIMER_LEFT_1_TM=58.590
PRIMER_RIGHT_1_TM=60.123
PRIMER_LEFT_1_GC_PERCENT=45.833
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=3.5510
PRIMER_LEFT_2_PENALTY=3.355446
PRIMER_RIGHT_2_PENALTY=0.195574
PRIMER_LEFT_2_SEQUENCE=TCCTTTACTACACTGTTCCTTCG
PRIMER_RIGHT_2_SEQUENCE=AACGCTTTGAGTTTTGAAGACC
PRIMER_LEFT_2=32,23
PRIMER_RIGHT_2=253,22
PRIMER_LEFT_2_TM=57.645
PRIMER_RIGHT_2_TM=59.804
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=3.6053
PRIMER_LEFT_3_PENALTY=3.409754
PRIMER_RIGHT_3_PENALTY=0.195574
PRIMER_LEFT_3_SEQUENCE=CTCCTTTACTACACTGTTCCTTCG
PRIMER_RIGHT_3_SEQUENCE=AACGCTTTGAGTTTTGAAGACC
PRIMER_LEFT_3=31,24
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=58.590
PRIMER_RIGHT_3_TM=59.804
PRIMER_LEFT_3_GC_PERCENT=45.833
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=3.6865
PRIMER_LEFT_4_PENALTY=3.355446
PRIMER_RIGHT_4_PENALTY=0.331013
PRIMER_LEFT_4_SEQUENCE=TCCTTTACTACACTGTTCCTTCG
PRIMER_RIGHT_4_SEQUENCE=CTTTGAGTTTTGAAGACCCGTT
PRIMER_LEFT_4=32,23
PRIMER_RIGHT_4=249,22
PRIMER_LEFT_4_TM=57.645
PRIMER_RIGHT_4_TM=59.669
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_05637 Ps_contig_mira-and-tgicl-ass_franssen_39075
SEQUENCE=CGATTCATTTCTCATCTTCTTCATTCTGCTCTCTCTCTGCATCCAATTCTCAATTTCGAATCAACCATGTCGCACTTCGGAAGATCCGGTCCTCCCGATATCTCCGACACCTACTCTCTACTCGTCCTCAACATCACTTTTCGTACAACCCCCGATGACCTATTTCCTCTCTTTGACAAATACGGCAAAGTTGTTGACATTTTCGTCCCCAAAGATAGAAGGACCGGTGAATCAAGAGGTTTCGCTTTTGTGCGTTACAAGTATGCTGATGAAGCTTCTAAGGCCGTTGATAGGCTCGACGGAAGAATGGTTGATGGTCGTGAAATAACTGTTCAGTTTGCGAAATATGGCCCTAATGCTGAGAGGATTCAGAAAGGAAGGATTATTGAAACATCCCCAAGATCGAAGAGCTCAAGGAGCCGCAGTCCCAGCAGGAGAAGGCACCGTGATGATTACAAAGGCAAGGATTACAGGAGGAGAAGTCGCAGCAGGAGTTATGATAGGTATGAACGCGACAGGTACCGCGGTGGGAGAGACAGGGACTATCGTCGCCGAAGCAGAAGCCGTAGTGCCAGTCCTGATCACAAGGGCCGTGGAAGGGGACGCTATGATGATGAGCGCCGTAGTGCCAGTCCTGATTACAAGGGCCGTGGAAGGGGACGCTATGATGATGAGCGCCGTAGTAGAAACCGAAGCAGATCAGTAGAAAGCCGATCCCCTGCCCAACGAAGTCCTAGTCCAATAAGGAGCCCTTCTCCTAAGAAGAGTATTTCCCCTCAAAGGAGTACTTCTCCCCAGAAAAGTCCACGTCATGAAAGTCCTGATAATCGTAGCCGTGATGAAAGGTCTCTTACTCCACGAAGTGTCTCACCACGAGGTCGCCCTGATGTTTCCCCAAGCCCATCTCCTTGAAATTCAAATGGCGATGAGTAAGCGGTTTTCAGGATGTATTCCAGAGACAACTGTACCTGTTGCTGTCTTGATTCGAACAATTTGGTCAGTGTGAATGGTCTTATCAGAGTCATTAAAGGAATTTATGGCCTAAGTGCAGTTTGGTAGGATTGGACTTTCTTGATGTTTAAATGTTCATATATTTTGACCTATATGTCATCTACTTTAGAATTTGGTAACTTTTGGGCCTTATAGTTGCTAAGCTAATATTTGACTATGAGGGATTTCGTCATTTTAGCCTTATTCTATTTGCATTTATCTCTCTGTTTCTGCTA
TARGET=29,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05638 Ps_contig_mira-and-tgicl-ass_franssen_39076
SEQUENCE=AACGCGTGACTCGTTCTCCTCTTCATCGTCATCGTCGCGCATCACCGTCGTGTTCTCCATAATCATCACTGTGCTCGCATCGCCGTCGCAGTCCTAGAAAGAAGAAGCTGAATCCGATGATGCGCATCCTCTTTCACTTGCGTTCGTTGCCTAGGACGACTCAAGGATTCATATCGCTTTCCACGTTCTGCAATCGCGCCTCGCAAACCTCGACTCACGGTTTCGTATTCGAAGATTCGATTTATGAAAAACAGAGATATGGAAGATGAATAAGAGTGCTTACCGATTTGGACAGCTATCGCGCGTTTTCCCGTTTGATTCCCGATTGATCTCTTCTTCTTCTTCTTCGGTTCGATGCTTTGCGGATTATAGCGGTTCTAAGAGTTGCGAACTGTTGCGAAAAGGATCGGCGTTTGTTCCGTTGCGTCGTTACGGTTGTTGCCGGTGGATTGCGATTTGCAGAACTCTTCACAAGTCCTTCTGTTGCGGTTATGCGGAACCGAGATTACCGTGAGGTTATGAGAGGATGAAGGTGAGAATGGAGAGCTCGAGAGTGAGTGCGTGGGAAGGTGGAGAGAAGAGAAAGATGATGAATGTGGCAATGGAAGAAAGTGAAGAGAGAAAAAGAAAAACCCCTTCTGAACGGTGAAGCTATNNGYTCGCNTTCAGTTAATAATGGAAAAGATGGAATTGTAGGTTTGTATTGAA
TARGET=332,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05639 Ps_contig_mira-and-tgicl-ass_franssen_39077
SEQUENCE=AAAAATAGTTGCGAATTAACCATTTACATTACTATTTTTTCTACAAGTCATATGCAATATCAAACAAATACATAAACATCATTCAACCAGTTAAAATTTTACAATTGTACCATCATAATAACATCATGTTCAATTTTCTCTTTATTGTTGAAAATAAAATAAGTACTAAGCTCTGTRTTTTTTTAATCTTGTTTTTGAGTTCATCTTTTTTGGCACCAACAATCTTATCCACCTCTTCCAATGCGACGGCCACCATAGCCTCCACCTCCACCATATCCACCACCACCACGTACCAACCCCGAAC
TARGET=277,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05640 Ps_contig_mira-and-tgicl-ass_franssen_39086
SEQUENCE=AAACTTAAAATAACCCTCATTTCTCTCTCTTAGAAAAAAAACTCNGAAGAAAGAAAAAAAACCAGTAAATCAACTCTCTCTTCTCTCACTCCTATTCACCTTTACTTTTAAAAATAAATAAATAAAAATGCTTCATGTTTCAAGCCAAACC
TARGET=112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05641 Ps_contig_mira-and-tgicl-ass_franssen_39094
SEQUENCE=TGTGATGGATTTCATGGAGAGGAGGTTTTTTCACTCCGACAATTGTAACATATAATACTGTCGTTGAAGTTTATGGAAAAGCCGGGGAAATTGAGAAGATGGATAAACACTTTAAGAAAATGAAGCATATAGGGATGAAACCTAATTCTGTAACGTATTGCTCGCTTGTCAGTGCGTATAGCAAAGCTGGGCTTATTGACAAAGTTGATTCAATCTTGAGGCATGTAGAAAATTCTGATGTTGTTTTGGACACTCCCTTCTTTAATTGCATAATTAGTGCTTATGCTCAAGCTGGCGACTTAAAAAAGATGGGCGAGTTATTCTTGGCAATGAAAGAAAGAAAATGCAGGCCTAATAACACCACCTTTGCGTGCATGATCCAGGCTTACAACACCCAAGG
TARGET=332,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3006
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=0.288468
PRIMER_LEFT_SEQUENCE=TGTTTTGGACACTCCCTTCTTT
PRIMER_RIGHT_SEQUENCE=ACGCAAAGGTGGTGTTATTAGG
PRIMER_LEFT=241,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=60.288
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=131
PRIMER_PAIR_PENALTY_1=0.3006
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=0.288468
PRIMER_LEFT_1_SEQUENCE=TTGTTTTGGACACTCCCTTCTT
PRIMER_RIGHT_1_SEQUENCE=ACGCAAAGGTGGTGTTATTAGG
PRIMER_LEFT_1=240,22
PRIMER_RIGHT_1=371,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=60.288
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=0.3910
PRIMER_LEFT_2_PENALTY=0.102564
PRIMER_RIGHT_2_PENALTY=0.288468
PRIMER_LEFT_2_SEQUENCE=GTCAGTGCGTATAGCAAAGCTG
PRIMER_RIGHT_2_SEQUENCE=ACGCAAAGGTGGTGTTATTAGG
PRIMER_LEFT_2=167,22
PRIMER_RIGHT_2=371,22
PRIMER_LEFT_2_TM=60.103
PRIMER_RIGHT_2_TM=60.288
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.4375
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=0.425301
PRIMER_LEFT_3_SEQUENCE=TGTTTTGGACACTCCCTTCTTT
PRIMER_RIGHT_3_SEQUENCE=CACGCAAAGGTGGTGTTATTAG
PRIMER_LEFT_3=241,22
PRIMER_RIGHT_3=372,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=59.575
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=0.4375
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=0.425301
PRIMER_LEFT_4_SEQUENCE=TTGTTTTGGACACTCCCTTCTT
PRIMER_RIGHT_4_SEQUENCE=CACGCAAAGGTGGTGTTATTAG
PRIMER_LEFT_4=240,22
PRIMER_RIGHT_4=372,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=59.575
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=133
=
PRIMER_SEQUENCE_ID=SSR_05642 Ps_contig_mira-and-tgicl-ass_franssen_39108
SEQUENCE=GAGATNTGAGAAAGACTAACTACCTATTTGAAGGACCGATATCATTGTCGTCGAGCGGATTTCTACTACTAGGAGGTGAACCAAATATTCGAAGGTCCGCCGCTCTAGTCGATGTGGCAGGAAGGCTGAAGGATGGAGGCAGAGAAGACGCAAGTGGTTGAAGAGCGGGGCTGAAACCATCACCGTCGCGTTCTTTGGCGTTGAACACCCGCTCGGACTCTGAGACTGAGATTCTTCTCCTCCAAATATATCATCAAAAAAATCTTTAGCTTTTTTGTTGTTTGTGTAACGTCTTCTAACTCCACTATCATCACCGAACACCGGTTTCTCTCTCTCACTCTCCACCGGCCACCTACACCAACCCGGTTCACCTTCTTCTCCACTGAACCCCGATGACCGCCGCGGTGGTCCCCCAAACACGTCGTTGAAATCAA
TARGET=327,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7033
PRIMER_LEFT_PENALTY=1.043090
PRIMER_RIGHT_PENALTY=0.660189
PRIMER_LEFT_SEQUENCE=AGAGAAGACGCAAGTGGTTGA
PRIMER_RIGHT_SEQUENCE=GGTTCAGTGGAGAAGAAGGTGA
PRIMER_LEFT=140,21
PRIMER_RIGHT=388,22
PRIMER_LEFT_TM=60.043
PRIMER_RIGHT_TM=60.660
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=1.8284
PRIMER_LEFT_1_PENALTY=1.168205
PRIMER_RIGHT_1_PENALTY=0.660189
PRIMER_LEFT_1_SEQUENCE=AAGGATGGAGGCAGAGAAGAC
PRIMER_RIGHT_1_SEQUENCE=GGTTCAGTGGAGAAGAAGGTGA
PRIMER_LEFT_1=128,21
PRIMER_RIGHT_1=388,22
PRIMER_LEFT_1_TM=59.832
PRIMER_RIGHT_1_TM=60.660
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=1.9796
PRIMER_LEFT_2_PENALTY=1.319394
PRIMER_RIGHT_2_PENALTY=0.660189
PRIMER_LEFT_2_SEQUENCE=CTCTAGTCGATGTGGCAGGAAG
PRIMER_RIGHT_2_SEQUENCE=GGTTCAGTGGAGAAGAAGGTGA
PRIMER_LEFT_2=102,22
PRIMER_RIGHT_2=388,22
PRIMER_LEFT_2_TM=61.319
PRIMER_RIGHT_2_TM=60.660
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=1.9980
PRIMER_LEFT_3_PENALTY=1.337822
PRIMER_RIGHT_3_PENALTY=0.660189
PRIMER_LEFT_3_SEQUENCE=GAAGACGCAAGTGGTTGAAGAG
PRIMER_RIGHT_3_SEQUENCE=GGTTCAGTGGAGAAGAAGGTGA
PRIMER_LEFT_3=143,22
PRIMER_RIGHT_3=388,22
PRIMER_LEFT_3_TM=61.338
PRIMER_RIGHT_3_TM=60.660
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=1.9980
PRIMER_LEFT_4_PENALTY=1.337822
PRIMER_RIGHT_4_PENALTY=0.660189
PRIMER_LEFT_4_SEQUENCE=AGAAGACGCAAGTGGTTGAAGA
PRIMER_RIGHT_4_SEQUENCE=GGTTCAGTGGAGAAGAAGGTGA
PRIMER_LEFT_4=142,22
PRIMER_RIGHT_4=388,22
PRIMER_LEFT_4_TM=61.338
PRIMER_RIGHT_4_TM=60.660
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_05643 Ps_contig_mira-and-tgicl-ass_franssen_39111
SEQUENCE=AGCTTTAGTAACGGGGGAAGTTTTATTGCAAAGAAGGAGCATTTTCCAGGATTTGCACGACCTTTTGTGTTCAATGCTGAGGTTATGTTGAGGGTTGGGCGTAAAGTAGAAGCTAAAGATGCTGCCAGGGGGAGCTTTGAAATCACCATGGTGGACTCTAGGTTGCATGTATCAGGTACGGAGTCTTTATTTAAGTATAACGTTATGATTCATCTTTCTTTCTTTTTAACGTATTCACATCTATTTCCTCCCTTTTCGGGTATTTCTTCCGTGAATTTTACTTGTTTAGGACGTTGCTAATATAGCCCAATGGGACGATGAGCAAATTGAGTATATTAAAGAGAAAGTGACTGAAGAGGGAAGACAAGAAGATCTTAAAAAGGGGAAGGCGCCTGCACAGGTTTCGTATAGTGTCAACTATTTATTATGGCTATCATTATCGAACATGCATGACAATGGCATAAGCTGGTACACACATAAATCTTTCTTATTT
TARGET=213,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0322
PRIMER_LEFT_PENALTY=0.022302
PRIMER_RIGHT_PENALTY=0.009871
PRIMER_LEFT_SEQUENCE=TGCATGTATCAGGTACGGAGTC
PRIMER_RIGHT_SEQUENCE=TGGGCTATATTAGCAACGTCCT
PRIMER_LEFT=163,22
PRIMER_RIGHT=308,22
PRIMER_LEFT_TM=60.022
PRIMER_RIGHT_TM=60.010
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=0.0604
PRIMER_LEFT_1_PENALTY=0.022302
PRIMER_RIGHT_1_PENALTY=0.038125
PRIMER_LEFT_1_SEQUENCE=TGCATGTATCAGGTACGGAGTC
PRIMER_RIGHT_1_SEQUENCE=ATACCCGAAAAGGGAGGAAATA
PRIMER_LEFT_1=163,22
PRIMER_RIGHT_1=262,22
PRIMER_LEFT_1_TM=60.022
PRIMER_RIGHT_1_TM=60.038
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=0.0663
PRIMER_LEFT_2_PENALTY=0.022302
PRIMER_RIGHT_2_PENALTY=0.043988
PRIMER_LEFT_2_SEQUENCE=TGCATGTATCAGGTACGGAGTC
PRIMER_RIGHT_2_SEQUENCE=GCATGTTCGATAATGATAGCCA
PRIMER_LEFT_2=163,22
PRIMER_RIGHT_2=448,22
PRIMER_LEFT_2_TM=60.022
PRIMER_RIGHT_2_TM=59.956
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=0.0663
PRIMER_LEFT_3_PENALTY=0.022302
PRIMER_RIGHT_3_PENALTY=0.043988
PRIMER_LEFT_3_SEQUENCE=TGCATGTATCAGGTACGGAGTC
PRIMER_RIGHT_3_SEQUENCE=TGCATGTTCGATAATGATAGCC
PRIMER_LEFT_3=163,22
PRIMER_RIGHT_3=449,22
PRIMER_LEFT_3_TM=60.022
PRIMER_RIGHT_3_TM=59.956
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=0.1181
PRIMER_LEFT_4_PENALTY=0.108206
PRIMER_RIGHT_4_PENALTY=0.009871
PRIMER_LEFT_4_SEQUENCE=ATGCTGAGGTTATGTTGAGGGT
PRIMER_RIGHT_4_SEQUENCE=TGGGCTATATTAGCAACGTCCT
PRIMER_LEFT_4=73,22
PRIMER_RIGHT_4=308,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=60.010
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=236
=
PRIMER_SEQUENCE_ID=SSR_05644 Ps_contig_mira-and-tgicl-ass_franssen_39124
SEQUENCE=TAAATCATGGCATNTGGATAACACAACGAAGAACACTTTCCTTCCTTTCATGAGGTTGAAAGCTTGTTAATGTCATCAAATAGCCAAATTGTGTGTTATGTAGTCATCAATCTCAATTTCCTGCACATTTATGTATCATATATATTATTAGCACATACTTTTATCATAGCATTCAATCAAACTTGTTTTACCTTGTTCATATATTTTTCTACTAGTGAAGGAAGATCGGTTTTAGGTTTCCATCCTCCAAATAGAGATCCTTTTAATGTTCTTCCTGTCAAGAATAGTCCATAATGAGCCGACATCTCAGGCTTCACCTTCGGCACACCAAGTGTTACAGTACCAAACCCGNTCCATCAACAACATGATTGACAATGCTAGTTTGTTATCATTATCCGACGGTTCCCGGTACCCCCCGAACTAACTAACTTTAACCGGAAAAACG
TARGET=419,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05645 Ps_contig_mira-and-tgicl-ass_franssen_39125
SEQUENCE=TCCTTCGGGGAGTGACGGCGACGANNAGAAAATAGGGCGGTGGAGTTTGGTAGGAAAGGAAAGGAATGATATATGTCCCTTACGATGGAACGCTATGTCCCCAAACTAATCNTAAATGGATGCAAAGTCCTTCCCTCCCTTCAAGCTACTGCATCTGAACCCCTTTCCAACAAGGGCATGAAAGGCAACTCTCATGGTCGCATGATCTCAACAAGGAGACTTTCCAAAAAGGGTATTGGAGACTAATATACTTGTTTATGATATTTGCAGCCACTATTGGTGAAGGTTTGTGGAAACTTCACAAATCAACATCTCCAAAGAGGTGACTTTGGAGTTGCAAATAACACAAGACAATAGTGAGGAGATGATTGGCTTGATGGGATCTTTCGCAAAACGGTCGGTATTGCAGGATTTTGGCTCTCGCCATGGACCGTCATCCATCATCGACAACAGTGTTATGTTTTGTTCCTCAAATTTGTTATGTTTATTGGAGCTGTTGTCATTCTAGTCTTGATGTTTACTCTACTC
TARGET=52,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0532
PRIMER_LEFT_PENALTY=2.038361
PRIMER_RIGHT_PENALTY=0.014792
PRIMER_LEFT_SEQUENCE=AGAAAATAGGGCGGTGGAGT
PRIMER_RIGHT_SEQUENCE=CCAATAGTGGCTGCAAATATCA
PRIMER_LEFT=26,20
PRIMER_RIGHT=279,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=59.985
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=2.0782
PRIMER_LEFT_1_PENALTY=2.038361
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=AGAAAATAGGGCGGTGGAGT
PRIMER_RIGHT_1_SEQUENCE=AGATGCAGTAGCTTGAAGGGAG
PRIMER_LEFT_1=26,20
PRIMER_RIGHT_1=155,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=2.1687
PRIMER_LEFT_2_PENALTY=2.038361
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=AGAAAATAGGGCGGTGGAGT
PRIMER_RIGHT_2_SEQUENCE=GAAAGGGGTTCAGATGCAGTAG
PRIMER_LEFT_2=26,20
PRIMER_RIGHT_2=166,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=141
PRIMER_PAIR_PENALTY_3=2.1687
PRIMER_LEFT_3_PENALTY=2.038361
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=AGAAAATAGGGCGGTGGAGT
PRIMER_RIGHT_3_SEQUENCE=GTTTCCACAAACCTTCACCAAT
PRIMER_LEFT_3=26,20
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=271
PRIMER_PAIR_PENALTY_4=2.1794
PRIMER_LEFT_4_PENALTY=2.164639
PRIMER_RIGHT_4_PENALTY=0.014792
PRIMER_LEFT_4_SEQUENCE=AAAATAGGGCGGTGGAGTTT
PRIMER_RIGHT_4_SEQUENCE=CCAATAGTGGCTGCAAATATCA
PRIMER_LEFT_4=28,20
PRIMER_RIGHT_4=279,22
PRIMER_LEFT_4_TM=59.835
PRIMER_RIGHT_4_TM=59.985
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=252
=
PRIMER_SEQUENCE_ID=SSR_05646 Ps_contig_mira-and-tgicl-ass_franssen_39150
SEQUENCE=TTCACATGAAGAAGTGAAAGAANAGTTCGGAGGTCTAGAACGGCAGAATGAAGATCCAAAGGGCGCGCCATGGTTGTTGGATCGAGGAGAAGTCGATGAGTGAAAACGATGATTCGGTGTTCCAGTATGAAGAACCGGGGTTCTGTGCGTTGAGCTTTTGTTTTTGCAGAAGAAAGGTGGATCCTTGCGTACGCGTCTGTGGCCGAGTGAAGATGATGAAGACTTTCCGGTAAGCTCTTGTTATTCCGTTTTTCTCCTGTTCTTGTTTTGGATCCTCCGTTTCGTTTTCTTCTTCTTCTGGGCGTTGATATGGTTCTTTGGAGTGGTGGTGAGGTTGGATTGAATTTGTTGAAGTGTTGGGTTTGATTGGCGTTTGGTGGTAGAGGTTTGTTGAATAAAAAAAATGGAGAGTTTCTTCTGTTATCGTAGGGTTTCTGTCAAGGGTTGTTAGCAGGGTGGTTCTCTGGATTGTCTAGAGGTTATGGTTGAGGGAGGTTATGTCTATGATAGGTTTGGTTGTGGACTCGTTCAGTTATTAGG
TARGET=287,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1229
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.099308
PRIMER_LEFT_SEQUENCE=CCTGTTCTTGTTTTGGATCCTC
PRIMER_RIGHT_SEQUENCE=CAACCCTTGACAGAAACCCTAC
PRIMER_LEFT=255,22
PRIMER_RIGHT=446,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.901
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=0.1229
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.099308
PRIMER_LEFT_1_SEQUENCE=CTGTTCTTGTTTTGGATCCTCC
PRIMER_RIGHT_1_SEQUENCE=CAACCCTTGACAGAAACCCTAC
PRIMER_LEFT_1=256,22
PRIMER_RIGHT_1=446,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.1229
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.099308
PRIMER_LEFT_2_SEQUENCE=CTGTTCTTGTTTTGGATCCTCC
PRIMER_RIGHT_2_SEQUENCE=ACAACCCTTGACAGAAACCCTA
PRIMER_LEFT_2=256,22
PRIMER_RIGHT_2=447,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=192
PRIMER_PAIR_PENALTY_3=0.1229
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.099308
PRIMER_LEFT_3_SEQUENCE=CCTGTTCTTGTTTTGGATCCTC
PRIMER_RIGHT_3_SEQUENCE=ACAACCCTTGACAGAAACCCTA
PRIMER_LEFT_3=255,22
PRIMER_RIGHT_3=447,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=59.901
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.2130
PRIMER_LEFT_4_PENALTY=0.117452
PRIMER_RIGHT_4_PENALTY=0.095574
PRIMER_LEFT_4_SEQUENCE=GTTGTTGGATCGAGGAGAAGTC
PRIMER_RIGHT_4_SEQUENCE=AAATTCAATCCAACCTCACCAC
PRIMER_LEFT_4=72,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=60.117
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_05647 Ps_contig_mira-and-tgicl-ass_franssen_39155
SEQUENCE=CTTTAACCTCCACCAGGAGCACCCTCTGTACCTGGTCAAGCAAATTCCCTTCAAAACCATACATGGATCCTCAAAGTAAACTACAACTAAGGATCCTTTATTGGATCTTATTACTTGTTTGTATTACTAATACTCTTCCAATCAAAGCTTTAGAACCAAGGAAGCTAGTTGAGAAGTGCACACCATGTGGTGACAATCCAATATCATCACCACCTCCAATAATATACAATTCACCACCACCAATTGTGTATTATCCATCACCACCACCACAAACCAAACCACTGCAATAACAACAACAGCTTCACAAGTTCCCCAACCTCCACAAAAGTCACCAAAACAAACTACGAAGACAGAACGAGATAGAGAACGTAACTTCTTCTACGGATAACAATGCTCTTACGTGGCAACATATCTCCTCCTCTCTCACTCTCTCTACAACTCAACCTCACAACACGAACCCACCCTCGAAACTTCACCCTTTCATCCCGATCAAGCCTAGACTACCCACTATCAATATCCGACGAGGCACTCGAGTCTCGAGGTTTCATTCTACGGCGAACCGCAGAGGGGCTAAACCTGGAAGCGCTGAACAAAGTATTCGTAGCGGTGGGGTTTCCGAGACGTGACCCGGAGAAGATACGAGTGGCGCTTGAGCACACGGACTCGATGGTGTGGGTGGAGCAGAGAAAGTCGCAGAAAGCAGTTGCGTTTGCGAGAGCTACGTGGGGATGGTTGTTTTACAATGCGAATTAAGTTTGGGATGCTATTTGACAAGCTAGGTGGGGGAGGTTCGTTGGAGGACAAAGTAGTTATCACGT
TARGET=259,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0604
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.045112
PRIMER_LEFT_SEQUENCE=TTCACCACCACCAATTGTGTAT
PRIMER_RIGHT_SEQUENCE=TGTTTTGGTGACTTTTGTGGAG
PRIMER_LEFT=229,22
PRIMER_RIGHT=338,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=60.045
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=110
PRIMER_PAIR_PENALTY_1=0.0675
PRIMER_LEFT_1_PENALTY=0.022341
PRIMER_RIGHT_1_PENALTY=0.045112
PRIMER_LEFT_1_SEQUENCE=ACCATGTGGTGACAATCCAATA
PRIMER_RIGHT_1_SEQUENCE=TGTTTTGGTGACTTTTGTGGAG
PRIMER_LEFT_1=181,22
PRIMER_RIGHT_1=338,22
PRIMER_LEFT_1_TM=59.978
PRIMER_RIGHT_1_TM=60.045
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=0.1248
PRIMER_LEFT_2_PENALTY=0.015253
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=TTCACCACCACCAATTGTGTAT
PRIMER_RIGHT_2_SEQUENCE=GATGAAAGGGTGAAGTTTCGAG
PRIMER_LEFT_2=229,22
PRIMER_RIGHT_2=484,22
PRIMER_LEFT_2_TM=60.015
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.1664
PRIMER_LEFT_3_PENALTY=0.015253
PRIMER_RIGHT_3_PENALTY=0.151106
PRIMER_LEFT_3_SEQUENCE=TTCACCACCACCAATTGTGTAT
PRIMER_RIGHT_3_SEQUENCE=GTGGGTAGTCTAGGCTTGATCG
PRIMER_LEFT_3=229,22
PRIMER_RIGHT_3=507,22
PRIMER_LEFT_3_TM=60.015
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.1745
PRIMER_LEFT_4_PENALTY=0.015253
PRIMER_RIGHT_4_PENALTY=0.159203
PRIMER_LEFT_4_SEQUENCE=TTCACCACCACCAATTGTGTAT
PRIMER_RIGHT_4_SEQUENCE=GATATGTTGCCACGTAAGAGCA
PRIMER_LEFT_4=229,22
PRIMER_RIGHT_4=412,22
PRIMER_LEFT_4_TM=60.015
PRIMER_RIGHT_4_TM=60.159
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_05648 Ps_contig_mira-and-tgicl-ass_franssen_39157
SEQUENCE=TAAACAACAAACAAACANCAGTTTTTCAAACCATACATATTTTTACATTACACAACAAGTGATTTATATAGAGAGAGACACACACAAACAACATAGGTGTTGTTTTTGYTTTGTTTGTGTGTGTGTGTGAGAGAGAGAAACAAAGAAGCTGTGGAAGTGTAAAAGAAGAAGATGAAGAACAAGAATGAATGAGTGAGGTGAAAGATGAAGGCTATGCCCTTACCTTTTGAAGAACACATAGGAAAAGGGGTGTTGGATTTTGGTTCAAGTGTTGGTAGTGTTGTTTCAGATTCGTTCTTATTTCAACAACAGCAACAACAGTTCTACAAAGGTGGAGGGAAAATTGTTGCTATGCGGGCATTGAGCCCAATTCTGTTGATGTTTTTGACCTCAAAAAAACCAAAATCCAGAAGCCACTCCTACTTCATCCTCAACGCTGTCTTCCTCACACGGCAGCGCCGCCGGAGGCGCTTCAGCCGATAGCAACAACAAGAACATACCTGAAGTTGAAGGAAAAAGATGTGGAATGGAAGATTGGGAGAGTGTGTTGTCTGAATCACCAAGTCAAGATCAAGATCATTCAATTTTGAAATTGATTATGGAAGATATTGAAGATGCATCTATGGGATTAAGCAAGCTTTTACAAGTTGGGACTAGTAATTCTCATTCTCATTCTCAGCAAGTATTGGTTTAGTAAATTTCTAAAGTTTTAGGGTTTTTTTAAGTTTTTAANAGGGGTAACTTAACCCTAAAAACCGTTAAA
TARGET=6,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05649 Ps_contig_mira-and-tgicl-ass_franssen_39157
SEQUENCE=TAAACAACAAACAAACANCAGTTTTTCAAACCATACATATTTTTACATTACACAACAAGTGATTTATATAGAGAGAGACACACACAAACAACATAGGTGTTGTTTTTGYTTTGTTTGTGTGTGTGTGTGAGAGAGAGAAACAAAGAAGCTGTGGAAGTGTAAAAGAAGAAGATGAAGAACAAGAATGAATGAGTGAGGTGAAAGATGAAGGCTATGCCCTTACCTTTTGAAGAACACATAGGAAAAGGGGTGTTGGATTTTGGTTCAAGTGTTGGTAGTGTTGTTTCAGATTCGTTCTTATTTCAACAACAGCAACAACAGTTCTACAAAGGTGGAGGGAAAATTGTTGCTATGCGGGCATTGAGCCCAATTCTGTTGATGTTTTTGACCTCAAAAAAACCAAAATCCAGAAGCCACTCCTACTTCATCCTCAACGCTGTCTTCCTCACACGGCAGCGCCGCCGGAGGCGCTTCAGCCGATAGCAACAACAAGAACATACCTGAAGTTGAAGGAAAAAGATGTGGAATGGAAGATTGGGAGAGTGTGTTGTCTGAATCACCAAGTCAAGATCAAGATCATTCAATTTTGAAATTGATTATGGAAGATATTGAAGATGCATCTATGGGATTAAGCAAGCTTTTACAAGTTGGGACTAGTAATTCTCATTCTCATTCTCAGCAAGTATTGGTTTAGTAAATTTCTAAAGTTTTAGGGTTTTTTTAAGTTTTTAANAGGGGTAACTTAACCCTAAAAACCGTTAAA
TARGET=116,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05650 Ps_contig_mira-and-tgicl-ass_franssen_39190
SEQUENCE=TATGTGTACAGACCAGGGTCAAGTTAGTTTGAAACATTTTTATTTATTTATATTATACTTGACATTTTGTTAAGATATTTCATTTTTTGTTGGTTTTAAAGGGTT
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05651 Ps_contig_mira-and-tgicl-ass_franssen_39192
SEQUENCE=CGTTTTAAAAAGAATTGATCAAACACATAGCATTATATATTTGTCAAATTACATTGAGACAAAAACAATAGTGTATGCTTATGAGAATTACTAAATATATCCACTAAGAAACTAGAAGACTCCATATTACAATATCCCTGTACATCATTGACCAATATATAGAGTGGTCACACTTGGAATCAGTGAACAATTGTGGGGTACTGACTTGCAAAAAAAAAATCCTACAAATTATATGCCCTACAATATGTCATAATGATCATTTTGGACCCTATGTCAGATACTTCTTTTAATTTCCACTTGGTCCTTCTCTTGTTCTCCCATGCTATAATATACATTATCATTAATTTCCATTTGCTCCTTCTTCCACAAGGAGGCCAAGTAGTAGATTGGTAAGAGTTGTTTTCTATCTTTATTATAGTTTCACATATTTAAGTATAAATTAAAAAAACACACAATGAAATCCATCTTTTATATGGAAGGAGCAACTAAAATAAATGTAAATTAATATTATCCATATATATGAAGGACTAATCAATATATATTTCCAAGTTATATTTTTAAAAACTTACTTTCAAAGTATATATTTTATTTTAAAATGTATTTTCTAACAATATATTCACATTTTCTTACCTAAAGGATTAAGAGTAGGAAAGTTTCTCTTTTAATTGATTTTCTTTTTAACTAGCAAAAGAGATAAAAAAAAACTCAACATGAAATCTAAGAGTTATATTTAGCCCATAAAATCAAATATATATTTAAGCCATTTTTTTCTTATGTTAGGAGAGAAATATATGAAAATACACACTCTCTCTCTCTCTCTATATATATATAAAGGTTGAATCAATATATATTTCCAAATTAAAAGACATGTATGCTTAGTTATTTATAGCTAGTGTAAGAAAAAAAACTCAAACTCAAGGCAGAAAGAAAAAATTAAAGCCATACTAATATAGATTTCCATATTAAAAAGTATGCTTATCTTTTCTAGCAAAAGAAAAGAAAATATCAATATGAAATATCTATATCTTAGTTAAATTTTGCAGAAGTTTTTTCATTACCAAAGCAAGAACCATATATAGAAATTTAAAGGAGAAAAAAATATTACAAAAGAACAAAGAGTATCAGTAGCACACAAGAACAACAAATTGACAAAGATTCATAACAAAAAATAGATAAAAGAGTGAAAAACAAATAAACCATCTAACAATTCGATCTATTTTTTAGTTTCGCTCACTATCTTTTTATATTTTTTAAGGGCTACAATACCCTTTATACTAAAATTATAATTAACAAAAAAACCTAGCACGATTATAAATTTTTCATATAACAAAGACTTATCTAAAACTGTAAAATATCAATGCCATATAGTAGCTGCAGATTTATATATAATAATTTATAAAATTGTATATATCTAAATATGTTTTGAAATTAAATTATTTAGAGTTAATTGAGAGTGAGAAAATGGAGGATAAAGATGAAGTGGATGACAGAAGTCTAGAGAGCACAAAGGAAATGAAATGCAATTAATTGACCCTTCATCTTCATCTCCATGTATCTATATGTATATCTATAAGCATATCATCCTCTGTGCTCTCTATCTTCTGTCAACAACTATATTTCCTCCCTCTCCCTCTTAAACAGTTTTTATCCCTTCCCTAATATTTATATAAAAAAGAAGAAAAGTGCATACAAAAACTAATCATAATATTAAAATAGCTAGAAGAAGAAACATAAAGAATAGAGAGTTTGTGTATTTGAAGTTTTGAAGTGTTTGTTGTTGTCCCGCGTAC
TARGET=805,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05652 Ps_contig_mira-and-tgicl-ass_franssen_39192
SEQUENCE=CGTTTTAAAAAGAATTGATCAAACACATAGCATTATATATTTGTCAAATTACATTGAGACAAAAACAATAGTGTATGCTTATGAGAATTACTAAATATATCCACTAAGAAACTAGAAGACTCCATATTACAATATCCCTGTACATCATTGACCAATATATAGAGTGGTCACACTTGGAATCAGTGAACAATTGTGGGGTACTGACTTGCAAAAAAAAAATCCTACAAATTATATGCCCTACAATATGTCATAATGATCATTTTGGACCCTATGTCAGATACTTCTTTTAATTTCCACTTGGTCCTTCTCTTGTTCTCCCATGCTATAATATACATTATCATTAATTTCCATTTGCTCCTTCTTCCACAAGGAGGCCAAGTAGTAGATTGGTAAGAGTTGTTTTCTATCTTTATTATAGTTTCACATATTTAAGTATAAATTAAAAAAACACACAATGAAATCCATCTTTTATATGGAAGGAGCAACTAAAATAAATGTAAATTAATATTATCCATATATATGAAGGACTAATCAATATATATTTCCAAGTTATATTTTTAAAAACTTACTTTCAAAGTATATATTTTATTTTAAAATGTATTTTCTAACAATATATTCACATTTTCTTACCTAAAGGATTAAGAGTAGGAAAGTTTCTCTTTTAATTGATTTTCTTTTTAACTAGCAAAAGAGATAAAAAAAAACTCAACATGAAATCTAAGAGTTATATTTAGCCCATAAAATCAAATATATATTTAAGCCATTTTTTTCTTATGTTAGGAGAGAAATATATGAAAATACACACTCTCTCTCTCTCTCTATATATATATAAAGGTTGAATCAATATATATTTCCAAATTAAAAGACATGTATGCTTAGTTATTTATAGCTAGTGTAAGAAAAAAAACTCAAACTCAAGGCAGAAAGAAAAAATTAAAGCCATACTAATATAGATTTCCATATTAAAAAGTATGCTTATCTTTTCTAGCAAAAGAAAAGAAAATATCAATATGAAATATCTATATCTTAGTTAAATTTTGCAGAAGTTTTTTCATTACCAAAGCAAGAACCATATATAGAAATTTAAAGGAGAAAAAAATATTACAAAAGAACAAAGAGTATCAGTAGCACACAAGAACAACAAATTGACAAAGATTCATAACAAAAAATAGATAAAAGAGTGAAAAACAAATAAACCATCTAACAATTCGATCTATTTTTTAGTTTCGCTCACTATCTTTTTATATTTTTTAAGGGCTACAATACCCTTTATACTAAAATTATAATTAACAAAAAAACCTAGCACGATTATAAATTTTTCATATAACAAAGACTTATCTAAAACTGTAAAATATCAATGCCATATAGTAGCTGCAGATTTATATATAATAATTTATAAAATTGTATATATCTAAATATGTTTTGAAATTAAATTATTTAGAGTTAATTGAGAGTGAGAAAATGGAGGATAAAGATGAAGTGGATGACAGAAGTCTAGAGAGCACAAAGGAAATGAAATGCAATTAATTGACCCTTCATCTTCATCTCCATGTATCTATATGTATATCTATAAGCATATCATCCTCTGTGCTCTCTATCTTCTGTCAACAACTATATTTCCTCCCTCTCCCTCTTAAACAGTTTTTATCCCTTCCCTAATATTTATATAAAAAAGAAGAAAAGTGCATACAAAAACTAATCATAATATTAAAATAGCTAGAAGAAGAAACATAAAGAATAGAGAGTTTGTGTATTTGAAGTTTTGAAGTGTTTGTTGTTGTCCCGCGTAC
TARGET=821,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05653 Ps_contig_mira-and-tgicl-ass_franssen_39218
SEQUENCE=CATATTCTTCTCCCTTTTTTTCTTATACAATGTAGAACAGTAAATCCAACACCGGCCTCAGGGAACAGGGCTCGGGTGTTACAATACAATACAACTATGTGAAAATATCATCAGCGCGAAAGAAACTACTACAGAGACTAATCTCTTGAGTTGGACATGACTGTAATAGCCGAGGATGCGACTTTAAGAAAGTGGCAATGACGGCTGGATCTTTTTAGGTATAAATAATGTGACATTATAACCACTATATAACAATATTAATAATTTTAGTATTCTATAATGATTCCTCCACCCATTGGAGATCTATGTGGAACACCTTTCCTTCTCTTAACTGTTCTGAAACTCACCACTGTTGGAGGTGGTGTTGAAGGTGTAAAGCTATGAATTCTGTTTCTTCCACCACTTCCATTGCTAATGCCATTCATTGACTCTTCACCATCAGACCCATTATTCTTCCTCAACTTCTTGGAGGTTCCAACAATCTTACAACCAAGAGAGCAGAAGCTGAAGGAATCAAGGAGGCTTCTGTCACAGACTTGGCAAGTATTGGTGACCCCTTTTCCAGGCCTAGGTTGAGGCCTTTCATTCAAAAACACAATCTTTGCACTGTTTATAACATAAGTTTGAACTTCTGTTATGTCCAGAAATTTCTGTATCTCAGAAACCCTTATCACATCATGGTATGAAGATCTCCTTATCTGAATTGTTCTGTGTTGTTTGTGAGAAGCAAGACAAGCAGAACAAAGGGCTCCATTGATACAATCCAAGCAAAACATGTTACATTCACTCTTGTGAGAATCTGAGTGGACTTTGCATTGAACAAAGAAACTCGTTTGAAGAAGTGGTTGAAGCCATAGCGGCCACTTGTTTTCTTCTTCTTCATAACCTCCCATGATTGAATGATAAGAATTTGAGTGGAAATTTGGAGGTGGAAAGAAAAAAAGAGAAGTTTTTTTTGTGTGTAGAAGTATGAATGGAGAGAGAGAAGGAAAAAGATTGGGAAATGGAAGTGCTTTTTTGTAGTGGCGGGTGAT
TARGET=80,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1577
PRIMER_LEFT_PENALTY=2.139861
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=AACAGTAAATCCAACACCGGC
PRIMER_RIGHT_SEQUENCE=CGGCTATTACAGTCATGTCCAA
PRIMER_LEFT=35,21
PRIMER_RIGHT=171,22
PRIMER_LEFT_TM=61.140
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=12.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=2.1587
PRIMER_LEFT_1_PENALTY=2.140832
PRIMER_RIGHT_1_PENALTY=0.017846
PRIMER_LEFT_1_SEQUENCE=ACAGTAAATCCAACACCGGC
PRIMER_RIGHT_1_SEQUENCE=CGGCTATTACAGTCATGTCCAA
PRIMER_LEFT_1=36,20
PRIMER_RIGHT_1=171,22
PRIMER_LEFT_1_TM=59.859
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=12.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=2.2690
PRIMER_LEFT_2_PENALTY=2.139861
PRIMER_RIGHT_2_PENALTY=0.129103
PRIMER_LEFT_2_SEQUENCE=AACAGTAAATCCAACACCGGC
PRIMER_RIGHT_2_SEQUENCE=CCACTTTCTTAAAGTCGCATCC
PRIMER_LEFT_2=35,21
PRIMER_RIGHT_2=194,22
PRIMER_LEFT_2_TM=61.140
PRIMER_RIGHT_2_TM=60.129
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=12.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=2.2699
PRIMER_LEFT_3_PENALTY=2.140832
PRIMER_RIGHT_3_PENALTY=0.129103
PRIMER_LEFT_3_SEQUENCE=ACAGTAAATCCAACACCGGC
PRIMER_RIGHT_3_SEQUENCE=CCACTTTCTTAAAGTCGCATCC
PRIMER_LEFT_3=36,20
PRIMER_RIGHT_3=194,22
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=60.129
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=12.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=2.2856
PRIMER_LEFT_4_PENALTY=2.139861
PRIMER_RIGHT_4_PENALTY=0.145769
PRIMER_LEFT_4_SEQUENCE=AACAGTAAATCCAACACCGGC
PRIMER_RIGHT_4_SEQUENCE=AGAGAAGGAAAGGTGTTCCACA
PRIMER_LEFT_4=35,21
PRIMER_RIGHT_4=327,22
PRIMER_LEFT_4_TM=61.140
PRIMER_RIGHT_4_TM=60.146
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=12.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_05654 Ps_contig_mira-and-tgicl-ass_franssen_39218
SEQUENCE=CATATTCTTCTCCCTTTTTTTCTTATACAATGTAGAACAGTAAATCCAACACCGGCCTCAGGGAACAGGGCTCGGGTGTTACAATACAATACAACTATGTGAAAATATCATCAGCGCGAAAGAAACTACTACAGAGACTAATCTCTTGAGTTGGACATGACTGTAATAGCCGAGGATGCGACTTTAAGAAAGTGGCAATGACGGCTGGATCTTTTTAGGTATAAATAATGTGACATTATAACCACTATATAACAATATTAATAATTTTAGTATTCTATAATGATTCCTCCACCCATTGGAGATCTATGTGGAACACCTTTCCTTCTCTTAACTGTTCTGAAACTCACCACTGTTGGAGGTGGTGTTGAAGGTGTAAAGCTATGAATTCTGTTTCTTCCACCACTTCCATTGCTAATGCCATTCATTGACTCTTCACCATCAGACCCATTATTCTTCCTCAACTTCTTGGAGGTTCCAACAATCTTACAACCAAGAGAGCAGAAGCTGAAGGAATCAAGGAGGCTTCTGTCACAGACTTGGCAAGTATTGGTGACCCCTTTTCCAGGCCTAGGTTGAGGCCTTTCATTCAAAAACACAATCTTTGCACTGTTTATAACATAAGTTTGAACTTCTGTTATGTCCAGAAATTTCTGTATCTCAGAAACCCTTATCACATCATGGTATGAAGATCTCCTTATCTGAATTGTTCTGTGTTGTTTGTGAGAAGCAAGACAAGCAGAACAAAGGGCTCCATTGATACAATCCAAGCAAAACATGTTACATTCACTCTTGTGAGAATCTGAGTGGACTTTGCATTGAACAAAGAAACTCGTTTGAAGAAGTGGTTGAAGCCATAGCGGCCACTTGTTTTCTTCTTCTTCATAACCTCCCATGATTGAATGATAAGAATTTGAGTGGAAATTTGGAGGTGGAAAGAAAAAAAGAGAAGTTTTTTTTGTGTGTAGAAGTATGAATGGAGAGAGAGAAGGAAAAAGATTGGGAAATGGAAGTGCTTTTTTGTAGTGGCGGGTGAT
TARGET=870,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0840
PRIMER_LEFT_PENALTY=0.040146
PRIMER_RIGHT_PENALTY=1.043868
PRIMER_LEFT_SEQUENCE=CTCCATTGATACAATCCAAGCA
PRIMER_RIGHT_SEQUENCE=CACCTCCAAATTTCCACTCAA
PRIMER_LEFT=748,22
PRIMER_RIGHT=930,21
PRIMER_LEFT_TM=59.960
PRIMER_RIGHT_TM=59.956
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=1.0860
PRIMER_LEFT_1_PENALTY=0.040146
PRIMER_RIGHT_1_PENALTY=1.045831
PRIMER_LEFT_1_SEQUENCE=CTCCATTGATACAATCCAAGCA
PRIMER_RIGHT_1_SEQUENCE=AGCACTTCCATTTCCCAATCT
PRIMER_LEFT_1=748,22
PRIMER_RIGHT_1=1013,21
PRIMER_LEFT_1_TM=59.960
PRIMER_RIGHT_1_TM=59.954
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=1.1950
PRIMER_LEFT_2_PENALTY=0.151106
PRIMER_RIGHT_2_PENALTY=1.043868
PRIMER_LEFT_2_SEQUENCE=GTTTGAAGAAGTGGTTGAAGCC
PRIMER_RIGHT_2_SEQUENCE=CACCTCCAAATTTCCACTCAA
PRIMER_LEFT_2=831,22
PRIMER_RIGHT_2=930,21
PRIMER_LEFT_2_TM=60.151
PRIMER_RIGHT_2_TM=59.956
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=100
PRIMER_PAIR_PENALTY_3=1.1969
PRIMER_LEFT_3_PENALTY=0.151106
PRIMER_RIGHT_3_PENALTY=1.045831
PRIMER_LEFT_3_SEQUENCE=GTTTGAAGAAGTGGTTGAAGCC
PRIMER_RIGHT_3_SEQUENCE=AGCACTTCCATTTCCCAATCT
PRIMER_LEFT_3=831,22
PRIMER_RIGHT_3=1013,21
PRIMER_LEFT_3_TM=60.151
PRIMER_RIGHT_3_TM=59.954
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=1.2165
PRIMER_LEFT_4_PENALTY=0.040146
PRIMER_RIGHT_4_PENALTY=1.176367
PRIMER_LEFT_4_SEQUENCE=CTCCATTGATACAATCCAAGCA
PRIMER_RIGHT_4_SEQUENCE=TCCCAATCTTTTTCCTTCTCTC
PRIMER_LEFT_4=748,22
PRIMER_RIGHT_4=1001,22
PRIMER_LEFT_4_TM=59.960
PRIMER_RIGHT_4_TM=58.824
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_05655 Ps_contig_mira-and-tgicl-ass_franssen_39218
SEQUENCE=CATATTCTTCTCCCTTTTTTTCTTATACAATGTAGAACAGTAAATCCAACACCGGCCTCAGGGAACAGGGCTCGGGTGTTACAATACAATACAACTATGTGAAAATATCATCAGCGCGAAAGAAACTACTACAGAGACTAATCTCTTGAGTTGGACATGACTGTAATAGCCGAGGATGCGACTTTAAGAAAGTGGCAATGACGGCTGGATCTTTTTAGGTATAAATAATGTGACATTATAACCACTATATAACAATATTAATAATTTTAGTATTCTATAATGATTCCTCCACCCATTGGAGATCTATGTGGAACACCTTTCCTTCTCTTAACTGTTCTGAAACTCACCACTGTTGGAGGTGGTGTTGAAGGTGTAAAGCTATGAATTCTGTTTCTTCCACCACTTCCATTGCTAATGCCATTCATTGACTCTTCACCATCAGACCCATTATTCTTCCTCAACTTCTTGGAGGTTCCAACAATCTTACAACCAAGAGAGCAGAAGCTGAAGGAATCAAGGAGGCTTCTGTCACAGACTTGGCAAGTATTGGTGACCCCTTTTCCAGGCCTAGGTTGAGGCCTTTCATTCAAAAACACAATCTTTGCACTGTTTATAACATAAGTTTGAACTTCTGTTATGTCCAGAAATTTCTGTATCTCAGAAACCCTTATCACATCATGGTATGAAGATCTCCTTATCTGAATTGTTCTGTGTTGTTTGTGAGAAGCAAGACAAGCAGAACAAAGGGCTCCATTGATACAATCCAAGCAAAACATGTTACATTCACTCTTGTGAGAATCTGAGTGGACTTTGCATTGAACAAAGAAACTCGTTTGAAGAAGTGGTTGAAGCCATAGCGGCCACTTGTTTTCTTCTTCTTCATAACCTCCCATGATTGAATGATAAGAATTTGAGTGGAAATTTGGAGGTGGAAAGAAAAAAAGAGAAGTTTTTTTTGTGTGTAGAAGTATGAATGGAGAGAGAGAAGGAAAAAGATTGGGAAATGGAAGTGCTTTTTTGTAGTGGCGGGTGAT
TARGET=977,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0860
PRIMER_LEFT_PENALTY=0.040146
PRIMER_RIGHT_PENALTY=1.045831
PRIMER_LEFT_SEQUENCE=CTCCATTGATACAATCCAAGCA
PRIMER_RIGHT_SEQUENCE=AGCACTTCCATTTCCCAATCT
PRIMER_LEFT=748,22
PRIMER_RIGHT=1013,21
PRIMER_LEFT_TM=59.960
PRIMER_RIGHT_TM=59.954
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=1.1969
PRIMER_LEFT_1_PENALTY=0.151106
PRIMER_RIGHT_1_PENALTY=1.045831
PRIMER_LEFT_1_SEQUENCE=GTTTGAAGAAGTGGTTGAAGCC
PRIMER_RIGHT_1_SEQUENCE=AGCACTTCCATTTCCCAATCT
PRIMER_LEFT_1=831,22
PRIMER_RIGHT_1=1013,21
PRIMER_LEFT_1_TM=60.151
PRIMER_RIGHT_1_TM=59.954
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=1.2172
PRIMER_LEFT_2_PENALTY=0.040146
PRIMER_RIGHT_2_PENALTY=1.177035
PRIMER_LEFT_2_SEQUENCE=CTCCATTGATACAATCCAAGCA
PRIMER_RIGHT_2_SEQUENCE=AAGCACTTCCATTTCCCAATCT
PRIMER_LEFT_2=748,22
PRIMER_RIGHT_2=1014,22
PRIMER_LEFT_2_TM=59.960
PRIMER_RIGHT_2_TM=61.177
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=267
PRIMER_PAIR_PENALTY_3=1.2172
PRIMER_LEFT_3_PENALTY=0.040146
PRIMER_RIGHT_3_PENALTY=1.177035
PRIMER_LEFT_3_SEQUENCE=CTCCATTGATACAATCCAAGCA
PRIMER_RIGHT_3_SEQUENCE=AGCACTTCCATTTCCCAATCTT
PRIMER_LEFT_3=748,22
PRIMER_RIGHT_3=1013,22
PRIMER_LEFT_3_TM=59.960
PRIMER_RIGHT_3_TM=61.177
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=1.2430
PRIMER_LEFT_4_PENALTY=0.197140
PRIMER_RIGHT_4_PENALTY=1.045831
PRIMER_LEFT_4_SEQUENCE=GAGAAGCAAGACAAGCAGAACA
PRIMER_RIGHT_4_SEQUENCE=AGCACTTCCATTTCCCAATCT
PRIMER_LEFT_4=721,22
PRIMER_RIGHT_4=1013,21
PRIMER_LEFT_4_TM=59.803
PRIMER_RIGHT_4_TM=59.954
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_05656 Ps_contig_mira-and-tgicl-ass_franssen_39220
SEQUENCE=AAGCACCCCTAGAAAACTCACCAATATCCATCAAATCAGAAACAAGCTTCTCTTGGAGCCTCCTATAACCATGGTAAACGCGGCCTCTTGACGAAGATGACAAATTCAAAGTCCACCAGAAAGATCCGACAAGAGCAAGAACAGCTATAAAAACTAAACTAAACTTCAATGAAAACAACAGCAACTTAAAACGGCTTCTTGGGGTGCCAACAATGAATGGATCAGATGCAAAACCATTCTCTTTATCTGAAAAAACCAACCTCAGAGGAGACCTCAATCCTAAAGGAGTGTGATCTGAAGAACCCCTTTTATCAAAATCATT
TARGET=151,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0318
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=TGACAAATTCAAAGTCCACCAG
PRIMER_RIGHT_SEQUENCE=AAAAGGGGTTCTTCAGATCACA
PRIMER_LEFT=97,22
PRIMER_RIGHT=309,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.0424
PRIMER_LEFT_1_PENALTY=0.018835
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=AGAAAGATCCGACAAGAGCAAG
PRIMER_RIGHT_1_SEQUENCE=AAAAGGGGTTCTTCAGATCACA
PRIMER_LEFT_1=117,22
PRIMER_RIGHT_1=309,22
PRIMER_LEFT_1_TM=60.019
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.0558
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.047586
PRIMER_LEFT_2_SEQUENCE=TGACAAATTCAAAGTCCACCAG
PRIMER_RIGHT_2_SEQUENCE=AAGAAGCCGTTTTAAGTTGCTG
PRIMER_LEFT_2=97,22
PRIMER_RIGHT_2=199,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=59.952
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.0558
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.047586
PRIMER_LEFT_3_SEQUENCE=TGACAAATTCAAAGTCCACCAG
PRIMER_RIGHT_3_SEQUENCE=CAAGAAGCCGTTTTAAGTTGCT
PRIMER_LEFT_3=97,22
PRIMER_RIGHT_3=200,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.952
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=104
PRIMER_PAIR_PENALTY_4=0.1318
PRIMER_LEFT_4_PENALTY=0.108206
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=AAGATCCGACAAGAGCAAGAAC
PRIMER_RIGHT_4_SEQUENCE=AAAAGGGGTTCTTCAGATCACA
PRIMER_LEFT_4=120,22
PRIMER_RIGHT_4=309,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_05657 Ps_contig_mira-and-tgicl-ass_franssen_39223
SEQUENCE=CTTTTYATATTACTTACTTGACAATTTTATTATCATTTCAAGCACACCATATACAACATAGTATTGGATAGTTGAATACATAAATTAACAATGCTCTGCACCTTTTCCCTGTTTTGATGATGATAAACAACTTTTCTGATTTTACAGACATAAAAGATAATTACTTTCACCACCTTATTTTGACATTATGGAACCTTAAAACGCAAAGTTCATCATGTCCATGCTCAAGCTTCGCATCATAACATTTTCCATGAATCCACATTTGCTGGGAGCCTTCTTAATGCTCTGGTTTCTTGCCTTGAAAGAGCATGAAGAAGAAGAAATAGGCACTATTGGGATATTCTCACAGTTGCAAAGTTCTACCATGTATCCATCTGGGTCATGGAAAAACACCTGCTCCACCTTGATTCCTTCATCCTCTACCAATGCTGTAACATACTTCATCCCCATTTCTTCCAACCTCATTTTCACAAGTCCAACGTCTGTACACTGGAATGATATGTGGTTGTCCTTGGGATTAATGGGTCGTAGGCTCATCCATAGGAGTATCATCAAATGCATCATTAGGTTTGGATTCTCAAGCGAGGGTTGTTAACTTACCCAAAAACCATTAATTATAACCAACCAGGCCTCCATTGAATTTAGAAAGGAGGAGGTCTTTAANGACGAACCCAAAACC
TARGET=311,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05658 Ps_contig_mira-and-tgicl-ass_franssen_39241
SEQUENCE=GTCGGGGTAGGGTTGCACTGGAGTGGAATGCCAAGTGAGAACCGTCTTTTAGCCCATGAACAGAAACATAACGGGAGAACATGAAAGTATGAAAAGCCCAAATTCTTAAAAAAGTTGAGGAGCACAGCATAGCATAGTATGGCGGCGCTGGCGAGGATTTTGCGGTTGCAGACGCGATTCGTTCCAACACAATCATTCCAATATCATCGCCACCCCATCGTCCAAGCTTCCTCTTGGCATTCTTCGATAGATACAATACTAAACAGATATGGATTTCTTAAAAGAGAGGTCTCGACACAGACAAATCCAAATAAACCCGTTTGTGAAGATGTAGGGAATAATGAAGCCGATACCTTGAAGTCTAGTGTAAACTCAGATAATGTCCCTACGTCCATGAGCATTACCGAGAACTCTTCCATAAAGTTTTCGGCTAATTCCAATTTGAAAACATCCTCAAGGCACGATCTTGCTATGATTTTCACCTGCAAGGTTTGTGAAACGAGGTCCATTAAGACAGTTTGTCGCGAATCATATGAGAAGGGTGTAGTAGTAGCAAGATGCGGTGGATGTAATAATCTTCACTTGCTTGCGGATCACCTTGGATGGTTTGGCGAACCAGGAAGCATTGAGGATTTTCTGGCTACTCAGGGAGAAGAAGTTAGAAGAGGGTCAGTTGATACACTAAATCTTACATTGGAAGATATAGCTGGAAAACAATCTTGAAAGGTACAATTTTTGACCAAATTTATATAGGCTGCATTAGTGTAGCACTGAGATATTGGAAGGGTGATGGAATGTAACAATTGTTGGTGTTCATTAAACTCAGACTTTTATTAAGTTTTGTGATTATTCTTGATATTAAGAAAATGTCTCATTCAATGTGATGTTATCTTTATTTATTTATGGTGGTGCATACTGAGATGTTTGTCACATTAAAAAATGTATAGGAGGGGAAGGAATG
TARGET=892,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1229
PRIMER_LEFT_PENALTY=0.108929
PRIMER_RIGHT_PENALTY=1.013951
PRIMER_LEFT_SEQUENCE=GCTGGAAAACAATCTTGAAAGG
PRIMER_RIGHT_SEQUENCE=AAACATCTCAGTATGCACCACC
PRIMER_LEFT=705,22
PRIMER_RIGHT=925,22
PRIMER_LEFT_TM=60.109
PRIMER_RIGHT_TM=58.986
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=1.1425
PRIMER_LEFT_1_PENALTY=0.090580
PRIMER_RIGHT_1_PENALTY=1.051910
PRIMER_LEFT_1_SEQUENCE=ATATTGGAAGGGTGATGGAATG
PRIMER_RIGHT_1_SEQUENCE=TGACAAACATCTCAGTATGCACC
PRIMER_LEFT_1=775,22
PRIMER_RIGHT_1=929,23
PRIMER_LEFT_1_TM=59.909
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=155
PRIMER_PAIR_PENALTY_2=1.1425
PRIMER_LEFT_2_PENALTY=0.090580
PRIMER_RIGHT_2_PENALTY=1.051910
PRIMER_LEFT_2_SEQUENCE=ATATTGGAAGGGTGATGGAATG
PRIMER_RIGHT_2_SEQUENCE=GACAAACATCTCAGTATGCACCA
PRIMER_LEFT_2=775,22
PRIMER_RIGHT_2=928,23
PRIMER_LEFT_2_TM=59.909
PRIMER_RIGHT_2_TM=60.052
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=1.1608
PRIMER_LEFT_3_PENALTY=0.108929
PRIMER_RIGHT_3_PENALTY=1.051910
PRIMER_LEFT_3_SEQUENCE=GCTGGAAAACAATCTTGAAAGG
PRIMER_RIGHT_3_SEQUENCE=GACAAACATCTCAGTATGCACCA
PRIMER_LEFT_3=705,22
PRIMER_RIGHT_3=928,23
PRIMER_LEFT_3_TM=60.109
PRIMER_RIGHT_3_TM=60.052
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=1.1608
PRIMER_LEFT_4_PENALTY=0.108929
PRIMER_RIGHT_4_PENALTY=1.051910
PRIMER_LEFT_4_SEQUENCE=GCTGGAAAACAATCTTGAAAGG
PRIMER_RIGHT_4_SEQUENCE=TGACAAACATCTCAGTATGCACC
PRIMER_LEFT_4=705,22
PRIMER_RIGHT_4=929,23
PRIMER_LEFT_4_TM=60.109
PRIMER_RIGHT_4_TM=60.052
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=225
=
PRIMER_SEQUENCE_ID=SSR_05659 Ps_contig_mira-and-tgicl-ass_franssen_39248
SEQUENCE=CAACTCCTCAATAACATCTTCATTAGTAAAAATTTTCTTTCTTTCCCTTTAGAATGTCTTCTCTTACCTACTAAGTTTAGTGTCTTTTGACTAATACTACGTTTGGGTTT
TARGET=34,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05660 Ps_contig_mira-and-tgicl-ass_franssen_39257
SEQUENCE=TTTGCTGCTTCTAAGTTAGGGACTGGTTGGTTTTTTGTTATGATTGTTGAGTTACGGCCCGCGAACTTTCCCCTTACCGTCGGGTCCCGAACCGAGATCCTTCCGACGGTTGGTTTTGAGTCGTTCCTTATAGTGAGTTTTTGAGTAAGATTAATAGTGATCAGGTTTTGAAAGTGGAGGTTGATGGGATTCGTTTAATGTTTAAGTTGAAGTCTGATTTGGAAGGTGGTGAAGTTTCTAGTAGTAATAGTAGTAGTAGGTTGAATCTTGAATCAGAGTCTTTGGTTATGAGTGTTGCCCCGACGAAGAGGATTGTTTATACTACAACTAGGCCTAGTGATATTAGAACTCCTTATGAAAAGA
TARGET=248,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2659
PRIMER_LEFT_PENALTY=0.012153
PRIMER_RIGHT_PENALTY=1.253706
PRIMER_LEFT_SEQUENCE=TCAGGTTTTGAAAGTGGAGGTT
PRIMER_RIGHT_SEQUENCE=GGGCAACACTCATAACCAAAGA
PRIMER_LEFT=160,22
PRIMER_RIGHT=299,22
PRIMER_LEFT_TM=60.012
PRIMER_RIGHT_TM=61.254
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=140
PRIMER_PAIR_PENALTY_1=1.2659
PRIMER_LEFT_1_PENALTY=0.012153
PRIMER_RIGHT_1_PENALTY=1.253706
PRIMER_LEFT_1_SEQUENCE=AGGTTTTGAAAGTGGAGGTTGA
PRIMER_RIGHT_1_SEQUENCE=GGGCAACACTCATAACCAAAGA
PRIMER_LEFT_1=162,22
PRIMER_RIGHT_1=299,22
PRIMER_LEFT_1_TM=60.012
PRIMER_RIGHT_1_TM=61.254
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=1.2773
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=1.253706
PRIMER_LEFT_2_SEQUENCE=GATCAGGTTTTGAAAGTGGAGG
PRIMER_RIGHT_2_SEQUENCE=GGGCAACACTCATAACCAAAGA
PRIMER_LEFT_2=158,22
PRIMER_RIGHT_2=299,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=61.254
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=1.3833
PRIMER_LEFT_3_PENALTY=0.012153
PRIMER_RIGHT_3_PENALTY=1.371104
PRIMER_LEFT_3_SEQUENCE=TCAGGTTTTGAAAGTGGAGGTT
PRIMER_RIGHT_3_SEQUENCE=GGCAACACTCATAACCAAAGAC
PRIMER_LEFT_3=160,22
PRIMER_RIGHT_3=298,22
PRIMER_LEFT_3_TM=60.012
PRIMER_RIGHT_3_TM=58.629
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=1.3833
PRIMER_LEFT_4_PENALTY=0.012153
PRIMER_RIGHT_4_PENALTY=1.371104
PRIMER_LEFT_4_SEQUENCE=AGGTTTTGAAAGTGGAGGTTGA
PRIMER_RIGHT_4_SEQUENCE=GGCAACACTCATAACCAAAGAC
PRIMER_LEFT_4=162,22
PRIMER_RIGHT_4=298,22
PRIMER_LEFT_4_TM=60.012
PRIMER_RIGHT_4_TM=58.629
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_05661 Ps_contig_mira-and-tgicl-ass_franssen_39265
SEQUENCE=GGGGGTTATCTAAGAAAAAAAAGGCAAAAAAGTATTTTGTAAGAAAAGAAAACTAAATATANAACCTAATTAATTGGAATTGGTGTTCAATTAATTTTCAACCATGTCATCTTCTTCTTCTTCGATTCTTGTAATCCAAATTCCAACAGCAACAACGGTTTCTGCTGTGAAGCAGATGGCACTTTTCAAACCTCTCAATCTGAATTTTCAGAAAGGCAGAGGCATCCAACGGCAGTTGAATCACCGCGCTCACCGCCACAGCTCTGTTAAGCCATTGCGGTTACGGTGCTCAACTCCTTCCAGTGGTGGGAACCTCCTCCATGACGATGCTATTGTTCTCCATGTTCGGGGAATGATGTGCGAGGGATGTGCTTCTAGCGTCAAGAAAATTCTAGAAACTCAACCAGAAGTGTTATCTGCTACTGTGAATTTAGCATCAGAGACAGCACTAGTTTC
TARGET=110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05662 Ps_contig_mira-and-tgicl-ass_franssen_39282
SEQUENCE=CGTTTAAATTAAAATCAGAGAAAGCTATATATTATTATTATAAATATCTATTCTGTTATAAAAATGGCAAAAACACTAATCCAGTTCTACATTTCAGTATCTGATTATACTACACATTAAGGGAAAAATCCCAACATCACAATCTCGTTCACTGTGTTGCAACACCCAACTTTCCTTCAACCAAGCTTGAACAAATGCTCCTTGTAAACTAAAACTACCTGTGGTACCCAGGCTCGACCCAATTTGATGTAAAATTCACATTTTTCAGAGCTCAGAGATCCTAGAGCAAGAAAACAAAAATGATCACCATTAATCATTGTTCCTTGAAAATGATGGCAACGGCACTGCACCTTGTGGACGAAGGATGACAAGCACACCAAACATCCCAAGCATTGGAAGAACAAGAAGCCATTTCAACAACTTATAGTAGTGCATATGGAATGTAAGTGAGAATTCGTCGGAGAAATATAGTCCTATTTTTCGTCCACCATCTCAAACCAAAACT
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05663 Ps_contig_mira-and-tgicl-ass_franssen_39288
SEQUENCE=TAACTCGGGGAAGGACGGCATCGTGCTCCCTCCTTCTCCGTCGCGCCGGAGTCACCTCTGGCGGCGGCGGGGTGATATCATCACCTTCTTCATACCATTAAAACCAGCTCTTCGTCCTCAACCTTAATCTGGTTTCAATTTCTACTAATTTCCAACCAAATCTTTAAATCACCGCTCACACTTAAAGAACCCTAACTCACTCAGCCTAAAATTAAATGACGAAACTCCTAAATTCAATCTCAAAACTTAGGGTAAATGATCCTCCAATTGAACCCTACGCAATGGTAACCAAATCAAGGTGAGAGGTGAAGGCAAAAAAACCTCTGAGGCGGAGCTCCGACGAAGGATTGAAACGAGGACGACGATTGCGACACGATGACTCCTGCAATCTAGTATATTCTTCTTCTTCCCTTCTATTCCCTTCTTCTCATATT
TARGET=398,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05664 Ps_contig_mira-and-tgicl-ass_franssen_39296
SEQUENCE=CTGTTGAACCAAGCATAGTATTCGAAAATAAGTGGTTGCTAAATTTCCATTTTCTTGTAGTTTCCGTGARNANAAAAAATTGCAGAGAATTTAGAATAAACAATATGGTTTAATTGGTCTGTTATTAGTGATCGATGATGTCGGCTCTTAAACTCACCGCAACCGCTTCTATCTACGTCCGTTCACCATCAACCGCCGCCACCGCCGCTGTCAAACCCCTCTTCCTCTCATTCTCAAACTCCTTCACCTCCAGAAACTTCCACCAACACAAACGCTTCAGATACACCTCCTCCGCAACTAAATTCCCAATTTCCAATAAACCCCCCTCTTTCTCCGCCAATTTGGGGCTTTCTGGAAAAGCCCTAGTTTCTACCGTTCCACCACAGCCAGAACCAGAACAAGAACAAGAACAACCACAATCTTCCAAATTGCTCACTCTACCCACCATTCTCACTCTTGGCCGTGTCGCCGCCGTGCCGCTTCTTGTTGCCACTTTCTATTTGGACGGTTGGCAAGGAACAGTAGCTACTACCRCAATCTTCATTGCTGCTTCTGTTACAGATTGGCTTGATGGTTACATTGCTCGCAAGATGAATTTAAAATCTTCATTTGGTGCATTCTTAGATCCCGTAGCTGATAAGCTTATGGTTGCTGCCACATTGGTTTTATTATGTACTAGACCTTTGGATGTTGGTTTGTTTGCACAAGCACCCTGGTTACTACCTATACCTGCCATTGCCATCATTGGTAGAGAGATTACCATGTCTGCTGTCAGGGAATGGGCTGCTTCCCAGGATAGTAAGCTTTTAGAGGCTGTTGCTGTTAATAACTTGGGGAAGTGGAAAACAGCCACACAGATGACTGCGTTAGCCATCCTCCTCGCCACCCGGGACTCGAGTCATGGAGGAACTGTTTTTGTAGTGGGATCTGGTGTTGCTTTGCTTTATACTTCAGCAGGGCTTGCTTTATGGTCATTGGTTGTATATATGAGGAAAATTTGGAAGGTGTTATTGAGGTAGCATACTTCATGCTCCATGTATTTCACATATATATCCAATTTTTTGCGGCTCCAATTCTCTTGTAGGTTACCATACTACCGATGTCTCGAAAGGGAGCTCCATTTTTGGGGGCATGTAAATGTAATATTTATTCTGTGCAATGTCTAACTAGACAGCATCCGCACACTATTTTTTGTCCTGTTATTCTAGTTCATAAAATGAAAGAAAGAAAGAAAGAAAGAAGACAACTGGATTTTGGAGTCTAAACTT
TARGET=1219,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05665 Ps_contig_mira-and-tgicl-ass_franssen_39298
SEQUENCE=TTATTTTCTGTGTTCATTGTTTGAATGGAAGCGGAAACAGAAGTTGAAGAGAAGCGGTGGTGCTTGCGACGGTTCTGTCATCGGCAATGGTGTTGGTTTAACGAGCAGGGAGGTTGAGAAGCGTAGGACAAGGAATTTTAGAGAGACGCAATCGGAGGTTGGAGAGTATGGTTTATCATTGGGAAGAAGATCATGTGATACTGATCCTAGGTTATCTGTGGATGATTCGAGGTTTTCATTCGATGCACCTCGGGCTTCTTGGGACGGTTATTTGATTGGGAAAGCGTGTCCGAGGTTTTCCCCTATGGTTGCTATTAATGGTGATAGGGTTTTGGTTGAGGAAGAAGAAGAAGGAGAAGAAGAGGTGGTGAATTTGGAAAGTGGTGGTAGTGGTGGTGGTGGAGAGCATTATCCTGGTGGTTCAGA
TARGET=341,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0516
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=AATGGTGATAGGGTTTTGGTTG
PRIMER_RIGHT_SEQUENCE=GAACCACCAGGATAATGCTCTC
PRIMER_LEFT=316,22
PRIMER_RIGHT=422,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=0.0804
PRIMER_LEFT_1_PENALTY=0.056850
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=AGGTTGAGAAGCGTAGGACAAG
PRIMER_RIGHT_1_SEQUENCE=CCAAATTCACCACCTCTTCTTC
PRIMER_LEFT_1=110,22
PRIMER_RIGHT_1=376,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.0804
PRIMER_LEFT_2_PENALTY=0.056850
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=AGGTTGAGAAGCGTAGGACAAG
PRIMER_RIGHT_2_SEQUENCE=TCCAAATTCACCACCTCTTCTT
PRIMER_LEFT_2=110,22
PRIMER_RIGHT_2=377,22
PRIMER_LEFT_2_TM=59.943
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.0992
PRIMER_LEFT_3_PENALTY=0.067436
PRIMER_RIGHT_3_PENALTY=0.031769
PRIMER_LEFT_3_SEQUENCE=TCATTGGGAAGAAGATCATGTG
PRIMER_RIGHT_3_SEQUENCE=GAACCACCAGGATAATGCTCTC
PRIMER_LEFT_3=175,22
PRIMER_RIGHT_3=422,22
PRIMER_LEFT_3_TM=59.933
PRIMER_RIGHT_3_TM=59.968
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=248
PRIMER_PAIR_PENALTY_4=0.1908
PRIMER_LEFT_4_PENALTY=0.167242
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=ATCTGTGGATGATTCGAGGTTT
PRIMER_RIGHT_4_SEQUENCE=TCCAAATTCACCACCTCTTCTT
PRIMER_LEFT_4=213,22
PRIMER_RIGHT_4=377,22
PRIMER_LEFT_4_TM=59.833
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_05666 Ps_contig_mira-and-tgicl-ass_franssen_39298
SEQUENCE=TTATTTTCTGTGTTCATTGTTTGAATGGAAGCGGAAACAGAAGTTGAAGAGAAGCGGTGGTGCTTGCGACGGTTCTGTCATCGGCAATGGTGTTGGTTTAACGAGCAGGGAGGTTGAGAAGCGTAGGACAAGGAATTTTAGAGAGACGCAATCGGAGGTTGGAGAGTATGGTTTATCATTGGGAAGAAGATCATGTGATACTGATCCTAGGTTATCTGTGGATGATTCGAGGTTTTCATTCGATGCACCTCGGGCTTCTTGGGACGGTTATTTGATTGGGAAAGCGTGTCCGAGGTTTTCCCCTATGGTTGCTATTAATGGTGATAGGGTTTTGGTTGAGGAAGAAGAAGAAGGAGAAGAAGAGGTGGTGAATTTGGAAAGTGGTGGTAGTGGTGGTGGTGGAGAGCATTATCCTGGTGGTTCAGA
TARGET=390,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8948
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.875033
PRIMER_LEFT_SEQUENCE=AATGGTGATAGGGTTTTGGTTG
PRIMER_RIGHT_SEQUENCE=TGAACCACCAGGATAATGCTCT
PRIMER_LEFT=316,22
PRIMER_RIGHT=423,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.875
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=108
PRIMER_PAIR_PENALTY_1=0.8948
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.875033
PRIMER_LEFT_1_SEQUENCE=AATGGTGATAGGGTTTTGGTTG
PRIMER_RIGHT_1_SEQUENCE=CTGAACCACCAGGATAATGCTC
PRIMER_LEFT_1=316,22
PRIMER_RIGHT_1=424,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=60.875
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.9012
PRIMER_LEFT_2_PENALTY=0.026163
PRIMER_RIGHT_2_PENALTY=0.875033
PRIMER_LEFT_2_SEQUENCE=TAGGGTTTTGGTTGAGGAAGAA
PRIMER_RIGHT_2_SEQUENCE=TGAACCACCAGGATAATGCTCT
PRIMER_LEFT_2=324,22
PRIMER_RIGHT_2=423,22
PRIMER_LEFT_2_TM=59.974
PRIMER_RIGHT_2_TM=60.875
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=100
PRIMER_PAIR_PENALTY_3=0.9012
PRIMER_LEFT_3_PENALTY=0.026163
PRIMER_RIGHT_3_PENALTY=0.875033
PRIMER_LEFT_3_SEQUENCE=TAGGGTTTTGGTTGAGGAAGAA
PRIMER_RIGHT_3_SEQUENCE=CTGAACCACCAGGATAATGCTC
PRIMER_LEFT_3=324,22
PRIMER_RIGHT_3=424,22
PRIMER_LEFT_3_TM=59.974
PRIMER_RIGHT_3_TM=60.875
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=101
PRIMER_PAIR_PENALTY_4=0.9425
PRIMER_LEFT_4_PENALTY=0.067436
PRIMER_RIGHT_4_PENALTY=0.875033
PRIMER_LEFT_4_SEQUENCE=TCATTGGGAAGAAGATCATGTG
PRIMER_RIGHT_4_SEQUENCE=TGAACCACCAGGATAATGCTCT
PRIMER_LEFT_4=175,22
PRIMER_RIGHT_4=423,22
PRIMER_LEFT_4_TM=59.933
PRIMER_RIGHT_4_TM=60.875
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_05667 Ps_contig_mira-and-tgicl-ass_franssen_39310
SEQUENCE=AGGCACTAGCAAGCAATCTCCAAGCAAGGAGGAGGAGGAGGAGGAGAAAAGTATCGACAAGCAATTTCCTAACAAAGAGAAGCAGAAGCGCACTAACAGTAACCAACATTCTTCAAGTAAAGAGGAAGAGAAATGCATTAACATGCATTCTACAAGCAAGGGGGGAAAGAAATTAGCTAGCATGCACTCTCCAAAAAATACTGCAGAGAAAGCTGATGAACGTCCTGAATGGTTGCCTGATGGCTGGGACATTAAAGTTCAAACCCGAAAAAGTGGTCCGGCTATGGGATCTTCATATAAGATCTACATTGATCCATTGAATATATATAAATTCTATTCCAAACCCGAGGTACTACGTCATCTTGAAACTACAAAGGCCAGCAACTGCTCTTCGAAGAAGAAGAAAGACACTAGCAAGCATTCTCCAAGCAAGGAGGAGGAGAAATGCACGGACAAACATTCTCCAAACAAGGAGGAGGAGAATTGCATTAACAAGCATTCTCTAAACAAAGAGAAGAAGAAGCGCACCAACAATAACCAACATTCTCCAAGTAAAGAGGAAGAGAAATGTATTAACATGCATTCTCCAAGCAAGGAGGAAGAAGAATACATCAACACGGATTA
TARGET=27,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5542
PRIMER_LEFT_PENALTY=1.416664
PRIMER_RIGHT_PENALTY=0.137489
PRIMER_LEFT_SEQUENCE=ACTAGCAAGCAATCTCCAAGCA
PRIMER_RIGHT_SEQUENCE=ATTCAGGACGTTCATCAGCTTT
PRIMER_LEFT=4,22
PRIMER_RIGHT=229,22
PRIMER_LEFT_TM=61.417
PRIMER_RIGHT_TM=60.137
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=226
PRIMER_PAIR_PENALTY_1=1.5542
PRIMER_LEFT_1_PENALTY=1.416664
PRIMER_RIGHT_1_PENALTY=0.137489
PRIMER_LEFT_1_SEQUENCE=GCACTAGCAAGCAATCTCCAAG
PRIMER_RIGHT_1_SEQUENCE=ATTCAGGACGTTCATCAGCTTT
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=229,22
PRIMER_LEFT_1_TM=61.417
PRIMER_RIGHT_1_TM=60.137
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=1.5542
PRIMER_LEFT_2_PENALTY=1.416664
PRIMER_RIGHT_2_PENALTY=0.137489
PRIMER_LEFT_2_SEQUENCE=CACTAGCAAGCAATCTCCAAGC
PRIMER_RIGHT_2_SEQUENCE=ATTCAGGACGTTCATCAGCTTT
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=229,22
PRIMER_LEFT_2_TM=61.417
PRIMER_RIGHT_2_TM=60.137
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=1.5754
PRIMER_LEFT_3_PENALTY=1.416664
PRIMER_RIGHT_3_PENALTY=0.158697
PRIMER_LEFT_3_SEQUENCE=ACTAGCAAGCAATCTCCAAGCA
PRIMER_RIGHT_3_SEQUENCE=GAACTTTAATGTCCCAGCCATC
PRIMER_LEFT_3=4,22
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=61.417
PRIMER_RIGHT_3_TM=59.841
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=1.5754
PRIMER_LEFT_4_PENALTY=1.416664
PRIMER_RIGHT_4_PENALTY=0.158697
PRIMER_LEFT_4_SEQUENCE=CACTAGCAAGCAATCTCCAAGC
PRIMER_RIGHT_4_SEQUENCE=GAACTTTAATGTCCCAGCCATC
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=259,22
PRIMER_LEFT_4_TM=61.417
PRIMER_RIGHT_4_TM=59.841
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_05668 Ps_contig_mira-and-tgicl-ass_franssen_39310
SEQUENCE=AGGCACTAGCAAGCAATCTCCAAGCAAGGAGGAGGAGGAGGAGGAGAAAAGTATCGACAAGCAATTTCCTAACAAAGAGAAGCAGAAGCGCACTAACAGTAACCAACATTCTTCAAGTAAAGAGGAAGAGAAATGCATTAACATGCATTCTACAAGCAAGGGGGGAAAGAAATTAGCTAGCATGCACTCTCCAAAAAATACTGCAGAGAAAGCTGATGAACGTCCTGAATGGTTGCCTGATGGCTGGGACATTAAAGTTCAAACCCGAAAAAGTGGTCCGGCTATGGGATCTTCATATAAGATCTACATTGATCCATTGAATATATATAAATTCTATTCCAAACCCGAGGTACTACGTCATCTTGAAACTACAAAGGCCAGCAACTGCTCTTCGAAGAAGAAGAAAGACACTAGCAAGCATTCTCCAAGCAAGGAGGAGGAGAAATGCACGGACAAACATTCTCCAAACAAGGAGGAGGAGAATTGCATTAACAAGCATTCTCTAAACAAAGAGAAGAAGAAGCGCACCAACAATAACCAACATTCTCCAAGTAAAGAGGAAGAGAAATGTATTAACATGCATTCTCCAAGCAAGGAGGAAGAAGAATACATCAACACGGATTA
TARGET=394,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1122
PRIMER_LEFT_PENALTY=0.088662
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=TGGGACATTAAAGTTCAAACCC
PRIMER_RIGHT_SEQUENCE=TCCTCCTTGTTTGGAGAATGTT
PRIMER_LEFT=244,22
PRIMER_RIGHT=476,22
PRIMER_LEFT_TM=60.089
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.1610
PRIMER_LEFT_1_PENALTY=0.137489
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=AAAGCTGATGAACGTCCTGAAT
PRIMER_RIGHT_1_SEQUENCE=TCCTCCTTGTTTGGAGAATGTT
PRIMER_LEFT_1=208,22
PRIMER_RIGHT_1=476,22
PRIMER_LEFT_1_TM=60.137
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=0.1912
PRIMER_LEFT_2_PENALTY=0.088662
PRIMER_RIGHT_2_PENALTY=0.102490
PRIMER_LEFT_2_SEQUENCE=TGGGACATTAAAGTTCAAACCC
PRIMER_RIGHT_2_SEQUENCE=CAATTCTCCTCCTCCTTGTTTG
PRIMER_LEFT_2=244,22
PRIMER_RIGHT_2=485,22
PRIMER_LEFT_2_TM=60.089
PRIMER_RIGHT_2_TM=60.102
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.1982
PRIMER_LEFT_3_PENALTY=0.174657
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=GAAACTACAAAGGCCAGCAACT
PRIMER_RIGHT_3_SEQUENCE=TCCTCCTTGTTTGGAGAATGTT
PRIMER_LEFT_3=364,22
PRIMER_RIGHT_3=476,22
PRIMER_LEFT_3_TM=59.825
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=0.2400
PRIMER_LEFT_4_PENALTY=0.137489
PRIMER_RIGHT_4_PENALTY=0.102490
PRIMER_LEFT_4_SEQUENCE=AAAGCTGATGAACGTCCTGAAT
PRIMER_RIGHT_4_SEQUENCE=CAATTCTCCTCCTCCTTGTTTG
PRIMER_LEFT_4=208,22
PRIMER_RIGHT_4=485,22
PRIMER_LEFT_4_TM=60.137
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_05669 Ps_contig_mira-and-tgicl-ass_franssen_39314
SEQUENCE=TCGGGGGGTAAGGTCCCTACTTGTTGTTCGAAATAGCGAAAACCAGAAATCCCAAATCTATGAACATTCAGAAATACTAGGTGCTAAAAAACTTATATGAGCAACTATAAATAAATAAACTATATATTATTGTTGAGCTATTGTACCGATCATAATCTGAAATCTCATACATATACTGAATATTTCTTTGCAGAAGAAGTTCGAAGCAGTGTTCATGGCTGGAATAACAGAAAACGGAAGAAGAATCATGGTAGCTATTGACGAAGGAGACGAGAGTATTTACGCTCTGACATGGAGTTTGAAGTAATTTGATTTTTACAAAATTCGAAAGATCATCTTATTCTCATCTACGTTAGCCTCTCGTGTTCGTTT
TARGET=107,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7025
PRIMER_LEFT_PENALTY=0.647580
PRIMER_RIGHT_PENALTY=0.054900
PRIMER_LEFT_SEQUENCE=AATAGCGAAAACCAGAAATCCC
PRIMER_RIGHT_SEQUENCE=CCATGATTCTTCTTCCGTTTTC
PRIMER_LEFT=31,22
PRIMER_RIGHT=250,22
PRIMER_LEFT_TM=60.648
PRIMER_RIGHT_TM=59.945
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.3000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.8488
PRIMER_LEFT_1_PENALTY=0.595491
PRIMER_RIGHT_1_PENALTY=0.253332
PRIMER_LEFT_1_SEQUENCE=GTTCGAAATAGCGAAAACCAGA
PRIMER_RIGHT_1_SEQUENCE=AGCTACCATGATTCTTCTTCCG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=255,22
PRIMER_LEFT_1_TM=60.595
PRIMER_RIGHT_1_TM=59.747
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.9003
PRIMER_LEFT_2_PENALTY=0.595491
PRIMER_RIGHT_2_PENALTY=0.304841
PRIMER_LEFT_2_SEQUENCE=GTTCGAAATAGCGAAAACCAGA
PRIMER_RIGHT_2_SEQUENCE=CAAACTCCATGTCAGAGCGTAA
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=60.595
PRIMER_RIGHT_2_TM=60.305
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.9009
PRIMER_LEFT_3_PENALTY=0.647580
PRIMER_RIGHT_3_PENALTY=0.253332
PRIMER_LEFT_3_SEQUENCE=AATAGCGAAAACCAGAAATCCC
PRIMER_RIGHT_3_SEQUENCE=AGCTACCATGATTCTTCTTCCG
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=60.648
PRIMER_RIGHT_3_TM=59.747
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=10.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=0.9524
PRIMER_LEFT_4_PENALTY=0.647580
PRIMER_RIGHT_4_PENALTY=0.304841
PRIMER_LEFT_4_SEQUENCE=AATAGCGAAAACCAGAAATCCC
PRIMER_RIGHT_4_SEQUENCE=CAAACTCCATGTCAGAGCGTAA
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=300,22
PRIMER_LEFT_4_TM=60.648
PRIMER_RIGHT_4_TM=60.305
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.3000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_05670 Ps_contig_mira-and-tgicl-ass_franssen_39316
SEQUENCE=TCGGGGACCTCTCTTTTCCCAAACCAAGACAAGATTCACGTGTCAGCCATGAATATCGCATTGCTCGCTTCGAATCATCTCTAGAGCTTACACCTCACCTCAACTCAACTCAAACACTCTCAATGTGCAAATAACAACACTCCTCTTCAACAATCCAAGAAGATGCTTCACCAGCCATTACTCTACAGTTCAAGAACCCTCACTCAACCAACACTCCTCGCTTCGATTTTCTCCGGCACCGGCAACAATCCCGGACGCACTTCACTTGCCGGAAATTTCTCTCACCGTGATATTCGGTTGGAGTTTAGTATGCAAGCATCTGGAAGCAAGTCGAGTAATAGTGGCCGTGGAAGCTTTAGTGAGACAAGAGAAGGCTTAAAGGTTAGAACAACTAATGCTAATGCTTATGGGCGTGCTCAAAGAA
TARGET=99,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4277
PRIMER_LEFT_PENALTY=0.332137
PRIMER_RIGHT_PENALTY=0.095538
PRIMER_LEFT_SEQUENCE=TTCCCAAACCAAGACAAGATTC
PRIMER_RIGHT_SEQUENCE=TAGAGTAATGGCTGGTGAAGCA
PRIMER_LEFT=15,22
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=60.332
PRIMER_RIGHT_TM=59.904
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=170
PRIMER_PAIR_PENALTY_1=0.4902
PRIMER_LEFT_1_PENALTY=0.394711
PRIMER_RIGHT_1_PENALTY=0.095538
PRIMER_LEFT_1_SEQUENCE=ATTCACGTGTCAGCCATGAATA
PRIMER_RIGHT_1_SEQUENCE=TAGAGTAATGGCTGGTGAAGCA
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=184,22
PRIMER_LEFT_1_TM=60.395
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=152
PRIMER_PAIR_PENALTY_2=0.4904
PRIMER_LEFT_2_PENALTY=0.332137
PRIMER_RIGHT_2_PENALTY=0.158297
PRIMER_LEFT_2_SEQUENCE=TTCCCAAACCAAGACAAGATTC
PRIMER_RIGHT_2_SEQUENCE=AGAGTAATGGCTGGTGAAGCAT
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=183,22
PRIMER_LEFT_2_TM=60.332
PRIMER_RIGHT_2_TM=60.158
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.5530
PRIMER_LEFT_3_PENALTY=0.394711
PRIMER_RIGHT_3_PENALTY=0.158297
PRIMER_LEFT_3_SEQUENCE=ATTCACGTGTCAGCCATGAATA
PRIMER_RIGHT_3_SEQUENCE=AGAGTAATGGCTGGTGAAGCAT
PRIMER_LEFT_3=33,22
PRIMER_RIGHT_3=183,22
PRIMER_LEFT_3_TM=60.395
PRIMER_RIGHT_3_TM=60.158
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.5530
PRIMER_LEFT_4_PENALTY=0.394711
PRIMER_RIGHT_4_PENALTY=0.158297
PRIMER_LEFT_4_SEQUENCE=ATTCACGTGTCAGCCATGAATA
PRIMER_RIGHT_4_SEQUENCE=AGTAATGGCTGGTGAAGCATCT
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=181,22
PRIMER_LEFT_4_TM=60.395
PRIMER_RIGHT_4_TM=60.158
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_05671 Ps_contig_mira-and-tgicl-ass_franssen_39318
SEQUENCE=AAAATACAAGAAATAAAAATAAAATAAAATAAATAAAAAATCACGTTCATTCTCTCTGAGTTTGTTTCTTCTCTAAGGCCTGGTTTCTTTGTTAAGATAACCAGGTCTTAACCAGCTCCAAATCCTTTTCCTTTTTCCTCTCACACTA
TARGET=16,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05672 Ps_contig_mira-and-tgicl-ass_franssen_39330
SEQUENCE=CGGTTTGAGCCTGTTTTCTTCTCTGATACAATTCCACATATTGTAATATTTGATAAACCTGATCCAATCTCACTATAGACAGACTTGAATCATGAGGAAAAAAGCAAGTGTGTATTCACAATCAGAAACTCTTGGTGAACACTGTCTTTTTGGTTTTGTAAAATGGGATTAACTGATTGAACATGTAACAACTGAGCAACACGGTCACCGCAATCATTGAAAAGCAATTCTCGATAATTTATGACACTTAAAATGTTCTTTGTGAATAGCAGTCAGCAGACCATCTCCACGGTTGTTGGTTCGAGCAAGCTTGATGAGTTGGTAGCCAGCATCCCCAAGTCTCTCCTCATACATGTGAACTAGTTCTTCACTTCCAACCCAAAATTCCTGTAGGCACATGATAGAAGATGACTCGGAAAGCAATGAATCAAGAATCCTTTGATTCCTAGCCAACCAAAATGATCTGAAATCACTTTCTCTAAGTCCTTGATTCTGTGGATCAATCCGTTTGTAAATAGGAGCGAGGATATTAAAAGTAGTGAATGAAACACAAGAAGAGTCTTTATCAACAACATCCACCATTGATGAAAAACAACCACTTTTATTAATTCTAGAAAATTCTCCATCACAGTTATTATTATTATTATAGTCATTAATAACAACATTATGAGAAGAACAAGAACCACTGTTCCCTCTTCTCAAATTGAACTTGGAGCCAGCAGCTACCACCATT
TARGET=632,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0264
PRIMER_LEFT_PENALTY=0.010077
PRIMER_RIGHT_PENALTY=0.016312
PRIMER_LEFT_SEQUENCE=ATCCGTTTGTAAATAGGAGCGA
PRIMER_RIGHT_SEQUENCE=GAGGGAACAGTGGTTCTTGTTC
PRIMER_LEFT=502,22
PRIMER_RIGHT=694,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.016
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=193
PRIMER_PAIR_PENALTY_1=0.0504
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.016312
PRIMER_LEFT_1_SEQUENCE=TCCTAGCCAACCAAAATGATCT
PRIMER_RIGHT_1_SEQUENCE=GAGGGAACAGTGGTTCTTGTTC
PRIMER_LEFT_1=443,22
PRIMER_RIGHT_1=694,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.016
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=252
PRIMER_PAIR_PENALTY_2=0.1554
PRIMER_LEFT_2_PENALTY=0.139129
PRIMER_RIGHT_2_PENALTY=0.016312
PRIMER_LEFT_2_SEQUENCE=CTGTGGATCAATCCGTTTGTAA
PRIMER_RIGHT_2_SEQUENCE=GAGGGAACAGTGGTTCTTGTTC
PRIMER_LEFT_2=492,22
PRIMER_RIGHT_2=694,22
PRIMER_LEFT_2_TM=59.861
PRIMER_RIGHT_2_TM=60.016
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.1558
PRIMER_LEFT_3_PENALTY=0.010077
PRIMER_RIGHT_3_PENALTY=0.145769
PRIMER_LEFT_3_SEQUENCE=ATCCGTTTGTAAATAGGAGCGA
PRIMER_RIGHT_3_SEQUENCE=GAAGAGGGAACAGTGGTTCTTG
PRIMER_LEFT_3=502,22
PRIMER_RIGHT_3=697,22
PRIMER_LEFT_3_TM=59.990
PRIMER_RIGHT_3_TM=60.146
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=0.1558
PRIMER_LEFT_4_PENALTY=0.010077
PRIMER_RIGHT_4_PENALTY=0.145769
PRIMER_LEFT_4_SEQUENCE=ATCCGTTTGTAAATAGGAGCGA
PRIMER_RIGHT_4_SEQUENCE=TTGAGAAGAGGGAACAGTGGTT
PRIMER_LEFT_4=502,22
PRIMER_RIGHT_4=701,22
PRIMER_LEFT_4_TM=59.990
PRIMER_RIGHT_4_TM=60.146
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=200
=
PRIMER_SEQUENCE_ID=SSR_05673 Ps_contig_mira-and-tgicl-ass_franssen_39331
SEQUENCE=GTTCGTTCCCTAAACGTACAGTTCCCTAATGACTTTAAATTCTAAGCTTTCCTCCTTTCATCGTAAGTGCACCAATGCTGAGGATTTTCGTGCATTTTTTTGCCTTTGTTGTCGCCTTTGAATTCAACAACCACCGAGGAACTCCTCTTCCTCGTATGTAAGAGCTCCTGCTTCCTCTTCCATTTTCTCATGAATTCGATTAACGCCATGTATCCTGATGCGTGTGGAAGCAAATTCCTCATACATTGGGCTTTGATTCCACTTTTTATTCGTTTAATTTTTGAAATTAGGATTTAGGGTTATTAAAATTTATGGCTCGGTTTAGATTTTGGGTTGGGAATTGATGAAAAACAAGGATATATCGAGATAGAGGGTGAGAAGACGCTTAGGATTGTGGTGATTTGGTCGTGATTTGACCTCCGCCGCCGCCGAAGGCGATTTCCGGCACGACGGAGTTTGTTCTCCAATGAAGTGGAAGGCTTTTGAATAAGTTGAGTGTCCGA
TARGET=420,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8542
PRIMER_LEFT_PENALTY=0.068749
PRIMER_RIGHT_PENALTY=0.785407
PRIMER_LEFT_SEQUENCE=ATTCGATTAACGCCATGTATCC
PRIMER_RIGHT_SEQUENCE=CTTCCACTTCATTGGAGAACAA
PRIMER_LEFT=193,22
PRIMER_RIGHT=477,22
PRIMER_LEFT_TM=60.069
PRIMER_RIGHT_TM=59.215
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.9531
PRIMER_LEFT_1_PENALTY=0.068749
PRIMER_RIGHT_1_PENALTY=0.884328
PRIMER_LEFT_1_SEQUENCE=ATTCGATTAACGCCATGTATCC
PRIMER_RIGHT_1_SEQUENCE=TTCATTGGAGAACAAACTCCGT
PRIMER_LEFT_1=193,22
PRIMER_RIGHT_1=470,22
PRIMER_LEFT_1_TM=60.069
PRIMER_RIGHT_1_TM=60.884
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.9661
PRIMER_LEFT_2_PENALTY=0.081801
PRIMER_RIGHT_2_PENALTY=0.884328
PRIMER_LEFT_2_SEQUENCE=ATACATTGGGCTTTGATTCCAC
PRIMER_RIGHT_2_SEQUENCE=TTCATTGGAGAACAAACTCCGT
PRIMER_LEFT_2=240,22
PRIMER_RIGHT_2=470,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=60.884
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.9894
PRIMER_LEFT_3_PENALTY=0.203971
PRIMER_RIGHT_3_PENALTY=0.785407
PRIMER_LEFT_3_SEQUENCE=ATTCCTCATACATTGGGCTTTG
PRIMER_RIGHT_3_SEQUENCE=CTTCCACTTCATTGGAGAACAA
PRIMER_LEFT_3=233,22
PRIMER_RIGHT_3=477,22
PRIMER_LEFT_3_TM=60.204
PRIMER_RIGHT_3_TM=59.215
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.9894
PRIMER_LEFT_4_PENALTY=0.203971
PRIMER_RIGHT_4_PENALTY=0.785407
PRIMER_LEFT_4_SEQUENCE=CAAATTCCTCATACATTGGGCT
PRIMER_RIGHT_4_SEQUENCE=CTTCCACTTCATTGGAGAACAA
PRIMER_LEFT_4=230,22
PRIMER_RIGHT_4=477,22
PRIMER_LEFT_4_TM=60.204
PRIMER_RIGHT_4_TM=59.215
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_05674 Ps_contig_mira-and-tgicl-ass_franssen_39341
SEQUENCE=AAAGATGAAACAGAAACCAATCTTGTTAATCTGAGGAGGACTATCTATCTAACAATCATGTCTAGTGTAGATTTCGAGGAAGCTGGTCATAAGCTTCTCAAAATTCATCTCGAGCCAGGTCAAGAGATGGAATTGTGCATTATGCTTTTGGAATGTTGCAGCCAAGAAAGAACTTACCTCCGATATTACGGTCTTCTGGGCCAGCGATTCTGCATGATTAACAAAGTGCATCAAGAAAATTTTGAGACATGCTTTGTGAAGCAATATTCCATGATCCATCGACTTGAAACAAACAAACTGCGAAATGTGGCAAAATTTTTTGCTCATTTGCTTGGGACATTTGCTCTGCCTTGGCACGTCTTATCATACATTCGCTTGACTGAAGAGGACACAACTTCTTCTTCGCGTATATTTATTAAGATTCTCTTCCAGGAATTGTCAGAGCATCTTGGCATTCGGTTGTTAAATGAGCGGTTAAATGATCCAACAATGCAGGATTCTTTTGAATCAATATTTCCCAAAGATAACCCTAAAAACACACGATTCTGCATTAACTTCTTCACATCCATTGGACTTGGTGGTCTTACTGAGAATCTACGCGAGTATTTGAAAAACATGCCACGTCTGATCATGCAACAACAGAAGCAAGTTCCTGATTCTGACTCAGATAATGAATCTGCAAGCTCCGGTTCATCAGATTCGGGAACCAGTTCTGAGTCAGAGTCTGACTCAGCAAGTTCTGATGAAAGTGATAGAAAAAGAAGTAAACGTAGAAGAAAATAAAGCATTGTATGCTATTGTCAAAACCCCTTCCTGCATCAGCAAACCCGCTGTTAGAGAATCCATGGTGGTTCCACANATCTCATGTCAGTGGATGTAACTTGTAACTTTTTAAATTGTTTCTAGGGAAGG
TARGET=287,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0377
PRIMER_LEFT_PENALTY=0.019805
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=CCTCCGATATTACGGTCTTCTG
PRIMER_RIGHT_SEQUENCE=TGTATGATAAGACGTGCCAAGG
PRIMER_LEFT=176,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=59.980
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=0.0377
PRIMER_LEFT_1_PENALTY=0.019805
PRIMER_RIGHT_1_PENALTY=0.017846
PRIMER_LEFT_1_SEQUENCE=CTCCGATATTACGGTCTTCTGG
PRIMER_RIGHT_1_SEQUENCE=TGTATGATAAGACGTGCCAAGG
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=369,22
PRIMER_LEFT_1_TM=59.980
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=0.1133
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=ATTTCGAGGAAGCTGGTCATAA
PRIMER_RIGHT_2_SEQUENCE=TGTATGATAAGACGTGCCAAGG
PRIMER_LEFT_2=70,22
PRIMER_RIGHT_2=369,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.1445
PRIMER_LEFT_3_PENALTY=0.019805
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=CCTCCGATATTACGGTCTTCTG
PRIMER_RIGHT_3_SEQUENCE=CAAGATGCTCTGACAATTCCTG
PRIMER_LEFT_3=176,22
PRIMER_RIGHT_3=450,22
PRIMER_LEFT_3_TM=59.980
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=0.1445
PRIMER_LEFT_4_PENALTY=0.019805
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=CTCCGATATTACGGTCTTCTGG
PRIMER_RIGHT_4_SEQUENCE=CAAGATGCTCTGACAATTCCTG
PRIMER_LEFT_4=177,22
PRIMER_RIGHT_4=450,22
PRIMER_LEFT_4_TM=59.980
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_05675 Ps_contig_mira-and-tgicl-ass_franssen_39343
SEQUENCE=TTTTTGNCTTTCAATACCGTTGTATGCAAGAGGAAGATGATGAAATCGAAACCGAAGAACCTACACAAGAGAACACCTCCCCAATCTCCACTTCCCCTCCTAGGTGTAATTTGGAATCAGCTACACCTCAAGATCCATCCACCTCCAGCCTCCGAGTATCTACACCCCAAGTTGCATCCACCTCCAGGTTCCGAGCACCTACACCTCGAGATGAAGTTCAGGTTCATACCCTCTTCTCCAACCCGCCTGAAGCCTTATCCATCGAGAAGTATTTGGAAGTCTTATCCTTCCAGATATCTCCACAGCCTACGGATGACGATTTTGGTAACGATCATTTTAAGGAACATGATGAAGATGATGAGGGTGGAAATCATAATGATGAAGGTGATGAGGGTAGAAATCATAATGATGATGAAGATGATGAGGGTGGCAATAATAATAATAATAATAATGCAAATGTTGGGGATGATGAGGTTGGCAATAATAATATTGCAGATGATGGGGATGATGAGGTTGGCAATAATAATAATGCAGATGATGGGGATGACAATCATAATTATGATGAGGGTGAGCATAATAATAATGTGAAGAATTATATACATGGTAG
TARGET=432,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3123
PRIMER_LEFT_PENALTY=0.265053
PRIMER_RIGHT_PENALTY=0.047267
PRIMER_LEFT_SEQUENCE=CTTCCAGATATCTCCACAGCCT
PRIMER_RIGHT_SEQUENCE=ATTATTGCCAACCTCATCATCC
PRIMER_LEFT=286,22
PRIMER_RIGHT=484,22
PRIMER_LEFT_TM=59.735
PRIMER_RIGHT_TM=60.047
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.3123
PRIMER_LEFT_1_PENALTY=0.265053
PRIMER_RIGHT_1_PENALTY=0.047267
PRIMER_LEFT_1_SEQUENCE=CTTCCAGATATCTCCACAGCCT
PRIMER_RIGHT_1_SEQUENCE=ATTATTGCCAACCTCATCATCC
PRIMER_LEFT_1=286,22
PRIMER_RIGHT_1=523,22
PRIMER_LEFT_1_TM=59.735
PRIMER_RIGHT_1_TM=60.047
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=238
PRIMER_PAIR_PENALTY_2=0.4712
PRIMER_LEFT_2_PENALTY=0.423908
PRIMER_RIGHT_2_PENALTY=0.047267
PRIMER_LEFT_2_SEQUENCE=TCAGGTTCATACCCTCTTCTCC
PRIMER_RIGHT_2_SEQUENCE=ATTATTGCCAACCTCATCATCC
PRIMER_LEFT_2=217,22
PRIMER_RIGHT_2=484,22
PRIMER_LEFT_2_TM=59.576
PRIMER_RIGHT_2_TM=60.047
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.4999
PRIMER_LEFT_3_PENALTY=0.081801
PRIMER_RIGHT_3_PENALTY=0.418063
PRIMER_LEFT_3_SEQUENCE=GGATGACGATTTTGGTAACGAT
PRIMER_RIGHT_3_SEQUENCE=ATGATTGTCATCCCCATCATCT
PRIMER_LEFT_3=310,22
PRIMER_RIGHT_3=553,22
PRIMER_LEFT_3_TM=60.082
PRIMER_RIGHT_3_TM=60.418
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=0.5348
PRIMER_LEFT_4_PENALTY=0.487583
PRIMER_RIGHT_4_PENALTY=0.047267
PRIMER_LEFT_4_SEQUENCE=CAGGTTCATACCCTCTTCTCCA
PRIMER_RIGHT_4_SEQUENCE=ATTATTGCCAACCTCATCATCC
PRIMER_LEFT_4=218,22
PRIMER_RIGHT_4=484,22
PRIMER_LEFT_4_TM=60.488
PRIMER_RIGHT_4_TM=60.047
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_05676 Ps_contig_mira-and-tgicl-ass_franssen_39343
SEQUENCE=TTTTTGNCTTTCAATACCGTTGTATGCAAGAGGAAGATGATGAAATCGAAACCGAAGAACCTACACAAGAGAACACCTCCCCAATCTCCACTTCCCCTCCTAGGTGTAATTTGGAATCAGCTACACCTCAAGATCCATCCACCTCCAGCCTCCGAGTATCTACACCCCAAGTTGCATCCACCTCCAGGTTCCGAGCACCTACACCTCGAGATGAAGTTCAGGTTCATACCCTCTTCTCCAACCCGCCTGAAGCCTTATCCATCGAGAAGTATTTGGAAGTCTTATCCTTCCAGATATCTCCACAGCCTACGGATGACGATTTTGGTAACGATCATTTTAAGGAACATGATGAAGATGATGAGGGTGGAAATCATAATGATGAAGGTGATGAGGGTAGAAATCATAATGATGATGAAGATGATGAGGGTGGCAATAATAATAATAATAATAATGCAAATGTTGGGGATGATGAGGTTGGCAATAATAATATTGCAGATGATGGGGATGATGAGGTTGGCAATAATAATAATGCAGATGATGGGGATGACAATCATAATTATGATGAGGGTGAGCATAATAATAATGTGAAGAATTATATACATGGTAG
TARGET=519,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4999
PRIMER_LEFT_PENALTY=0.081801
PRIMER_RIGHT_PENALTY=0.418063
PRIMER_LEFT_SEQUENCE=GGATGACGATTTTGGTAACGAT
PRIMER_RIGHT_SEQUENCE=ATGATTGTCATCCCCATCATCT
PRIMER_LEFT=310,22
PRIMER_RIGHT=553,22
PRIMER_LEFT_TM=60.082
PRIMER_RIGHT_TM=60.418
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=244
PRIMER_PAIR_PENALTY_1=0.6831
PRIMER_LEFT_1_PENALTY=0.265053
PRIMER_RIGHT_1_PENALTY=0.418063
PRIMER_LEFT_1_SEQUENCE=CTTCCAGATATCTCCACAGCCT
PRIMER_RIGHT_1_SEQUENCE=ATGATTGTCATCCCCATCATCT
PRIMER_LEFT_1=286,22
PRIMER_RIGHT_1=553,22
PRIMER_LEFT_1_TM=59.735
PRIMER_RIGHT_1_TM=60.418
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.9488
PRIMER_LEFT_2_PENALTY=0.530753
PRIMER_RIGHT_2_PENALTY=0.418063
PRIMER_LEFT_2_SEQUENCE=GATATCTCCACAGCCTACGGAT
PRIMER_RIGHT_2_SEQUENCE=ATGATTGTCATCCCCATCATCT
PRIMER_LEFT_2=292,22
PRIMER_RIGHT_2=553,22
PRIMER_LEFT_2_TM=59.469
PRIMER_RIGHT_2_TM=60.418
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.8000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=262
PRIMER_PAIR_PENALTY_3=1.2320
PRIMER_LEFT_3_PENALTY=0.081801
PRIMER_RIGHT_3_PENALTY=1.150203
PRIMER_LEFT_3_SEQUENCE=GGATGACGATTTTGGTAACGAT
PRIMER_RIGHT_3_SEQUENCE=GATTGTCATCCCCATCATCTG
PRIMER_LEFT_3=310,22
PRIMER_RIGHT_3=551,21
PRIMER_LEFT_3_TM=60.082
PRIMER_RIGHT_3_TM=60.150
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.0000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=1.2320
PRIMER_LEFT_4_PENALTY=0.081801
PRIMER_RIGHT_4_PENALTY=1.150203
PRIMER_LEFT_4_SEQUENCE=GGATGACGATTTTGGTAACGAT
PRIMER_RIGHT_4_SEQUENCE=TGATTGTCATCCCCATCATCT
PRIMER_LEFT_4=310,22
PRIMER_RIGHT_4=552,21
PRIMER_LEFT_4_TM=60.082
PRIMER_RIGHT_4_TM=60.150
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_05677 Ps_contig_mira-and-tgicl-ass_franssen_39359
SEQUENCE=TTAATAATAAAAGAGCAAGTGNCCAATAGTACTGATCGTAAATTAGAAGAAACCACACTTCTAGTTATAGATGATTCGTGTTGCAAAATACACAACCAGTTCACATACTTTTCAGAGTTGAATTTCGCTTTTCCACGAAGAGAAGACATTTTTTTTATTATTTCCAAAAGACAAAAGAAAAAGTTTACCTATAATCATCAAGACATAATGTACAGTTTTGTTAACCTTGTATCACTTGAAGGAATTGCTATTGTTCTCAGGAGCAACATAAAATGCTTTTGTCGCCGCTATAGCAGAATCTCCTCTCTTGACAATTTTCTCAAGCTCCTCAACCTTCTCCAACAAGGAAGCAACATTTGAATTGGTACATTGAACCAGCTTGTTTGCAGTATCTAGAGAAGTACATAACTTCAAAGTGAGAGAAGTCCAAGAATGATCGGTAGCATTGAGAGCTTCGTTAAGGTGATAGTAGTTTTCCTGAAAGAGTTTGGTGAGGTCAGGAAGTGGTTGGTTGTTGTTGTTGAGATCTGAAAGAGAAGAAGGATTAGAAGAGGGATGATAGGAGGAAGAGGTTGTTCCTCCATCTTCATTTTCTTCTCCGACGTCGCTGAGGAGATCTCCGATGCATAAAAGGGGTCCTCCGATTCCTCTCATTTTCTCTTTTCCGATCTAAGTTTTTCTGCAGCTTGTGTTTATCGGTATTTTTCTTCTTCTTTTCCTTTGAAACCCTAGACTCAATTCAATCAGGTTAGCGGAACTGATGATGAAATGGAGAAGAAAACAAAACCTATCACATATTTTTGTTATAAAACAAGTTTGCATCGAA
TARGET=511,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1124
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.110131
PRIMER_LEFT_SEQUENCE=TTTCTCAAGCTCCTCAACCTTC
PRIMER_RIGHT_SEQUENCE=TGAAGATGGAGGAACAACCTCT
PRIMER_LEFT=315,22
PRIMER_RIGHT=588,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.1910
PRIMER_LEFT_1_PENALTY=0.080887
PRIMER_RIGHT_1_PENALTY=0.110131
PRIMER_LEFT_1_SEQUENCE=GCATTGAGAGCTTCGTTAAGGT
PRIMER_RIGHT_1_SEQUENCE=TGAAGATGGAGGAACAACCTCT
PRIMER_LEFT_1=442,22
PRIMER_RIGHT_1=588,22
PRIMER_LEFT_1_TM=59.919
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.1998
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.197454
PRIMER_LEFT_2_SEQUENCE=TTTCTCAAGCTCCTCAACCTTC
PRIMER_RIGHT_2_SEQUENCE=AACCTCTTCCTCCTATCATCCC
PRIMER_LEFT_2=315,22
PRIMER_RIGHT_2=573,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=59.803
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.2058
PRIMER_LEFT_3_PENALTY=0.095629
PRIMER_RIGHT_3_PENALTY=0.110131
PRIMER_LEFT_3_SEQUENCE=CAAGAATGATCGGTAGCATTGA
PRIMER_RIGHT_3_SEQUENCE=TGAAGATGGAGGAACAACCTCT
PRIMER_LEFT_3=427,22
PRIMER_RIGHT_3=588,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=0.2414
PRIMER_LEFT_4_PENALTY=0.145769
PRIMER_RIGHT_4_PENALTY=0.095629
PRIMER_LEFT_4_SEQUENCE=GAAAGAGTTTGGTGAGGTCAGG
PRIMER_RIGHT_4_SEQUENCE=CATTTCATCATCAGTTCCGCTA
PRIMER_LEFT_4=479,22
PRIMER_RIGHT_4=770,22
PRIMER_LEFT_4_TM=60.146
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_05678 Ps_contig_mira-and-tgicl-ass_franssen_39366
SEQUENCE=GGGGGATCCCTGAGAAGAANCAGANNACGCTAAAGAATCCAAACGCTCAGCCGCACCTCTCTCGCGAACGCGATGCCATCCTCTTCACCGCGACTCAACGTGGAAGCAAACGACTTGATCGACTCCACCATAATCTCACAGAGTCGCTAAGGAAATGAAGAATCGTGAACGTGAATTGATCCTCTCGTGAAGAATCTCAAGAACACAACTCTGAATCCGTGTTCACTCGTCCCCGAACCGCGGTCAATCAAGATTAAAATTATTCAACGGTTCACTCTTACGCGGGGAATGAAATCAATTGAAGGATAAAATCGTACCGGAGTCGCGTGGTGTGCGGAGAAAGAGTCGGATTTGTGCTCGTCGCGTGGAACTGAAGACGAAGAGAACAAAACTATCCTTGAATCGTGAGGAAGACAACGTTGAAGGATGAAGAATCTAAGGAAGGTGACGCAGTTCTTGTGTGGTTGCGGTGTGGAATCGTGATAGCGGTTCGGTTTCTACTTGCAAGAATCGAAACACTAAGGTGGCGCGACGGAGAAATAGGCTCGAACGAAGATGATGATGGAAGAAGGTTCGATGGTCGTTGGTGAGTTCTCACCGTGATGATGGAGGTTGTTGCAGGTGAGGTTGAAGAATTGGAAGAGAGTTTTTGATGAAGGTGATTGTGAATTGAAAAACGGTTACGGTTACGATGGTTGAAGGTTTTGAAGAGGAATTCTTGGATGGATTCGCAGTGGTTGAAGGTTTGGTGAAGGTGGAGATTACCGTTACGGTTACGGATTTGCAATGGTTGAAGGTTGAGAGTTTGTGTTGAAGAAGAAGAAGTTTATGATGAGGTTGAAGAATCTGGAAGTTTGAGAAGTGAAGATTGAAGAAGAATTGAATCGAGGAAAAAAACTGGATGAAAAGAGATGAATTGCTTTTATAGAGTGATGAGAGGTTAGAAGAAAYTTGTTAGAAGAAAATTCTGTTATTGGATTTGTTATTTCTTCTGTGAAATTATGCAGGTTCACTGTTAGTTGCAGTTTTTAATTGAAACTGTCAATTTCTGTTTGCAGGACTTTTTGTTGAAGGNCTAAAAATGGTTTTTATTGCCGGCGTTAAACCGT
TARGET=813,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05679 Ps_contig_mira-and-tgicl-ass_franssen_39367
SEQUENCE=GGGGTTTCGAACGCAGAGTACGCGGGGACGGGACAAAAATAATTTCAATTTCCACTATAACAGTCTCTTCTTCTCTTCATCTATATCTATCTCTCCAACTGCTACCACCCTTACAACCACCACCACCGCCGATTCTAAGGTTCAACGAGTAACTAAGAAATCTTCCGATGAACTACTTCGAAAATTTGCTGAAGTGGGTACCAACGAGAAGAAAGAGCTACGTATTATCAAACGCAGGAAGAAGATGAAGGAGAATCAACGCGAGAGCCAGACGCCCTTCGAAAGCCGGCGCCGCGTTGTGAAGGNGCNTGTTCGCCCGACCAACGTACGGTAAACCGGGTGGGTTCGGTTGTTCGCCGTCCGGCAGGTCGTTAGG
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0924
PRIMER_LEFT_PENALTY=0.054021
PRIMER_RIGHT_PENALTY=0.038361
PRIMER_LEFT_SEQUENCE=CTCCAACTGCTACCACCCTTAC
PRIMER_RIGHT_SEQUENCE=TTCTCCTTCATCTTCTTCCTGC
PRIMER_LEFT=92,22
PRIMER_RIGHT=254,22
PRIMER_LEFT_TM=60.054
PRIMER_RIGHT_TM=59.962
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.2692
PRIMER_LEFT_1_PENALTY=0.054021
PRIMER_RIGHT_1_PENALTY=0.215225
PRIMER_LEFT_1_SEQUENCE=CTCCAACTGCTACCACCCTTAC
PRIMER_RIGHT_1_SEQUENCE=GCGTTGATTCTCCTTCATCTTC
PRIMER_LEFT_1=92,22
PRIMER_RIGHT_1=261,22
PRIMER_LEFT_1_TM=60.054
PRIMER_RIGHT_1_TM=60.215
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.3364
PRIMER_LEFT_2_PENALTY=0.054021
PRIMER_RIGHT_2_PENALTY=0.282356
PRIMER_LEFT_2_SEQUENCE=CTCCAACTGCTACCACCCTTAC
PRIMER_RIGHT_2_SEQUENCE=GTTGGTACCCACTTCAGCAAAT
PRIMER_LEFT_2=92,22
PRIMER_RIGHT_2=204,22
PRIMER_LEFT_2_TM=60.054
PRIMER_RIGHT_2_TM=60.282
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=0.5822
PRIMER_LEFT_3_PENALTY=0.054021
PRIMER_RIGHT_3_PENALTY=0.528219
PRIMER_LEFT_3_SEQUENCE=CTCCAACTGCTACCACCCTTAC
PRIMER_RIGHT_3_SEQUENCE=TCTTCTCGTTGGTACCCACTTC
PRIMER_LEFT_3=92,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=60.054
PRIMER_RIGHT_3_TM=60.528
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=0.5984
PRIMER_LEFT_4_PENALTY=0.054021
PRIMER_RIGHT_4_PENALTY=0.544368
PRIMER_LEFT_4_SEQUENCE=CTCCAACTGCTACCACCCTTAC
PRIMER_RIGHT_4_SEQUENCE=CGTAGCTCTTTCTTCTCGTTGG
PRIMER_LEFT_4=92,22
PRIMER_RIGHT_4=221,22
PRIMER_LEFT_4_TM=60.054
PRIMER_RIGHT_4_TM=60.544
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_05680 Ps_contig_mira-and-tgicl-ass_franssen_39368
SEQUENCE=TGCTGTTGATGTTCTTGGTGCTGCTGCTGCTGTCTCGAAATAATGTTTTGATTAGTAGATTGAATCTGTCTACTTGTTTTCCATCTAATATATTCCATCAAGAGAGAATTCACGCTGATCGAAATCCCGAATCCGGTGAATGTTGAAAGAAGAACCGAGAATATCACAGTGACATTAAGTATTGAATAGAAAACATGAGTAAACAGGATGACAAATGCAAACTGGAAGCATGCATAAGCCCATATGTAACTCTTGCTAACCATTCTTGATGAGATTAAAGAATAGAAAAGGAAGAACCTAAGATGCATGAGAAAAGGAAAACAA
TARGET=19,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05681 Ps_contig_mira-and-tgicl-ass_franssen_39373
SEQUENCE=TTCGTAACAACGTAGGGCAAAGGACTCGGTTTTCGTATCAAACCCTACGATNACGGCGAGCGAAGATCTCTTTGTTTCATCAAYCATCCATCAGATCTGATGGATTCCGCACCCTTGCCGAAGGCGATCGTGTCGAATTCACCATCGCCACCGGCGACAATGAAAAAACCAAGGCTGTTGATGTCACCGGTCCTAACGGCGCTCCTCTGCAGTCCAGGCAGGACTCTTATGGCGGTGGAGGTGGCGGCCGTGGATTCGGTGGTGGATTTAGGGGTGCTGAGAGACGTAACGGCGGCGGCGGCGGCGGCGCTGGCTGTTACAACTGTGGTGACACTGGTCACATCGCTAGAGAGTGTAACCGGAGCAATAATTCCGGTGGAGGTGGAGGTGGAGGCGCTGCTGGTGCCGGCTGTTACAATTGCGGTGACACTGGTCACATCGCTAGGGATTGCAACCGGATCAATAATTCCGGTGGTGGTGGAGGAGGTGCTTGTTATACCTGCGGCTCTTTTGGTCATATAGCTAGGGATTGCACTCGCGGAGGCAATATTGGAGGTGGATTTGACCGCAGCAGTGGAGGCGCCACTTCTTGCTACAGATGTGGTGGAATCGGGCACATTGCAAGAGACTGCGCCACTCCCAGCGACCGAAGTGGCGGAGGTGGTGGATGCTATAAGTGCGGTGAAGTTGGTCACATAGCCAGGGATTGCAACATTGAAGGTGGCAGGTTCGATGGTGGCAACGGTGGTGGAAGATTTGGTGGTGGCAACAATGGAAGGTTTGGTGGTGGCAATGGAGGAACCAACACATGCTTCAATTGTGGGAAGCCAGGTCATTTTGCTAGGGAGTGCGTTGAGGCTTCTGTTTGAAGGAAAAAGGTGGAATAGTACATATTCTCTTTTTTGCACATTTAACAGTTTTTAACTGATTAGTATTTCTTTTATATATTCTTATAATTTTGGGAAGCAAGTTTGTTTCTATACATAGGATGCTATGACGATTATTATGGTTTTGGTTCTGTGTTTTTGACTTTGGTTCATTGCCAATTGGGGCTTCTTTTGTGGTTGGTTTATATATCTGATAATGCTTATTAGACCTTATTTATGA
TARGET=290,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05682 Ps_contig_mira-and-tgicl-ass_franssen_39374
SEQUENCE=GTCGGGGATCATGGAGAACAACAACAACAACCACAACGAACACCAACAAACCCAATATCCAACACCGCAACCCACGGCGGGCACTCCCCCGCCATTCCAACACCTCTTACAGCAACAACAACAACAGCTCCAAATGTTCTGGTCCTACCAAAGACAAGAAATCTGCTTTCAGTATTCATTTTGAATGGGACAGTAACATGGGAATTCCAGGAGCTTTTTATATTGRGAATTATTTAAAAGATACATAAAACTTTGGGGGAGAGAGATTAAGAAATTTTCTGTGCAAAACAAAGAATACAATTTCCGTTTTTCCGCCACCAGAGTTGAACACGGCGCAAATTCGCCGTCGGATCCAGAGTCACGCCACTCTTCACTCCACCCATCTCTTCATTCCCCCAAAACTCCAATACCTAAAACCTACCTCTCTCTCCTTTTCTGCTTCATCCACGATCTGCAATCTTGGGTTCAGGACATAGGAGCTGGGGGGAACCTGACATTATGAATGTTGTCTGGACATTGACCAGAGCAATCTATTATTGGAATTGATAAAAAGTAGTTATTTGGATATCACAAACAAATTAAACAACGAAATAATGGCTTCACACAAAACTGCCCAAATTCGCCTTGTTAGTTCACATGACGAGGTTTATGAACCATTGTGATGTACTTTCTTTTGACTACGTTGGGTTCGACCGTTCCTT
TARGET=17,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05683 Ps_contig_mira-and-tgicl-ass_franssen_39374
SEQUENCE=GTCGGGGATCATGGAGAACAACAACAACAACCACAACGAACACCAACAAACCCAATATCCAACACCGCAACCCACGGCGGGCACTCCCCCGCCATTCCAACACCTCTTACAGCAACAACAACAACAGCTCCAAATGTTCTGGTCCTACCAAAGACAAGAAATCTGCTTTCAGTATTCATTTTGAATGGGACAGTAACATGGGAATTCCAGGAGCTTTTTATATTGRGAATTATTTAAAAGATACATAAAACTTTGGGGGAGAGAGATTAAGAAATTTTCTGTGCAAAACAAAGAATACAATTTCCGTTTTTCCGCCACCAGAGTTGAACACGGCGCAAATTCGCCGTCGGATCCAGAGTCACGCCACTCTTCACTCCACCCATCTCTTCATTCCCCCAAAACTCCAATACCTAAAACCTACCTCTCTCTCCTTTTCTGCTTCATCCACGATCTGCAATCTTGGGTTCAGGACATAGGAGCTGGGGGGAACCTGACATTATGAATGTTGTCTGGACATTGACCAGAGCAATCTATTATTGGAATTGATAAAAAGTAGTTATTTGGATATCACAAACAAATTAAACAACGAAATAATGGCTTCACACAAAACTGCCCAAATTCGCCTTGTTAGTTCACATGACGAGGTTTATGAACCATTGTGATGTACTTTCTTTTGACTACGTTGGGTTCGACCGTTCCTT
TARGET=113,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05684 Ps_contig_mira-and-tgicl-ass_franssen_39381
SEQUENCE=CTACAAAAACATTCAAAACGACGCGCGCTGAATTCAATTTAACNGTTACGACTTTCTCTCTGAGACTTCGACTGTGCTAAGGGAAATTGAATTGAGAAATCCATGGCGCCGCACGATCTCCGCCGTCCGTTCAAACGACCGTTGATCTCCGATCAAGAGAGGCGCAGACAACAGTCTCTACTCCGCCAGGCACAGAATCGCCTCGACGCTCAGCGTCAGGCTCGCTTCTTAGCCTCCACCGCATTCTCTCTCTCATCCCAAACCCCCGAACCCGAACCCGAACCCGTGAACGAACCTGAGGTATATCAGAAAGAGGAACTCGACGTG
TARGET=245,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6631
PRIMER_LEFT_PENALTY=0.055352
PRIMER_RIGHT_PENALTY=0.607713
PRIMER_LEFT_SEQUENCE=AGACAACAGTCTCTACTCCGCC
PRIMER_RIGHT_SEQUENCE=ACGTCGAGTTCCTCTTTCTGAT
PRIMER_LEFT=165,22
PRIMER_RIGHT=325,22
PRIMER_LEFT_TM=59.945
PRIMER_RIGHT_TM=59.392
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=12.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=161
PRIMER_PAIR_PENALTY_1=0.8243
PRIMER_LEFT_1_PENALTY=0.216588
PRIMER_RIGHT_1_PENALTY=0.607713
PRIMER_LEFT_1_SEQUENCE=CTTCGACTGTGCTAAGGGAAAT
PRIMER_RIGHT_1_SEQUENCE=ACGTCGAGTTCCTCTTTCTGAT
PRIMER_LEFT_1=65,22
PRIMER_RIGHT_1=325,22
PRIMER_LEFT_1_TM=59.783
PRIMER_RIGHT_1_TM=59.392
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.9027
PRIMER_LEFT_2_PENALTY=0.294975
PRIMER_RIGHT_2_PENALTY=0.607713
PRIMER_LEFT_2_SEQUENCE=ACTTCGACTGTGCTAAGGGAAA
PRIMER_RIGHT_2_SEQUENCE=ACGTCGAGTTCCTCTTTCTGAT
PRIMER_LEFT_2=64,22
PRIMER_RIGHT_2=325,22
PRIMER_LEFT_2_TM=60.295
PRIMER_RIGHT_2_TM=59.392
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=262
PRIMER_PAIR_PENALTY_3=0.9284
PRIMER_LEFT_3_PENALTY=0.320679
PRIMER_RIGHT_3_PENALTY=0.607713
PRIMER_LEFT_3_SEQUENCE=TGAGACTTCGACTGTGCTAAGG
PRIMER_RIGHT_3_SEQUENCE=ACGTCGAGTTCCTCTTTCTGAT
PRIMER_LEFT_3=60,22
PRIMER_RIGHT_3=325,22
PRIMER_LEFT_3_TM=59.679
PRIMER_RIGHT_3_TM=59.392
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=1.0434
PRIMER_LEFT_4_PENALTY=0.435722
PRIMER_RIGHT_4_PENALTY=0.607713
PRIMER_LEFT_4_SEQUENCE=AGACTTCGACTGTGCTAAGGGA
PRIMER_RIGHT_4_SEQUENCE=ACGTCGAGTTCCTCTTTCTGAT
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=325,22
PRIMER_LEFT_4_TM=60.436
PRIMER_RIGHT_4_TM=59.392
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_05685 Ps_contig_mira-and-tgicl-ass_franssen_39383
SEQUENCE=TCGGGGATAAATATTTATATACATTTCATACANGACGGAGAAACACATAATTTCCTCACAATTTTCTTCTTCTCTCTCTCTCTCTCTGCAACCAACAAGCTTGAAACAGAAGA
TARGET=71,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05686 Ps_contig_mira-and-tgicl-ass_franssen_39396
SEQUENCE=AGTNCTCGTCGCCGGCACACACACTCTCTCGAACTCTCTCGGTAGCAGCAACACTATGTCGGCAACGGAGCTTCCACTTCTCGCTTCTGAAAACACCGCCGTGTTTCTATCCTCGGTTGCTGAAGCTTTGGTTCTTCAAACTGTCATTGCTACCGGCAAATCCCTCGCTTACCTTCTCATTGCGACTGGATCTCTTCTTACAGATGTCAGTAATTTGATATCACCAATGGATGGAAGGTTTCCTTTAGACCAGCTGTACAAGGGAAACCATACTTGTGAAGAGAACAAAGATGGAAGTGAAACAGAGGATGATGACGACGATGACGATGACGATGATGTGAATGACGAGGATGATGATGACGATGATGAGGACTTCTCTGGTGATGAAGAGGACGATGAAGCCGACCCTGAGGATGATCCTGTGCCTAACGGTGCAGGAGGAAGTGACGACGATGATGATGATGAGGACGGAGATGATGATGATGGTGACGACGAGGATGATGAAGACGAGGATGAAGACGAAGAAGAGGATGAAGATGAAGAAGTGGCGGCTCTTCAACCACCTTCTAAGAAGAAGAAATAAGTGTTATATTAGTAGGTAAATTGTTTTCTTCCTGCCTGTTGAGCACTTTTTATTTTGTAGCGACGTGTAGTTATTGGGATGCTGTGACATATCATCTATCTTGCCTATGGCCAACATTAAGATTTACAAACACCATCGTTAATATATTATGATGTATCCCTTGTTAATTTATTGGTGTTGCTTGTTGTGATAGTATGATTTTTATTGTTGCCGAGCATTCAAATTTGATGTTAATCCGATACACCTTTTCTCATTAGAGTTTTGAGATTGGACCTTTT
TARGET=452,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2369
PRIMER_LEFT_PENALTY=0.156061
PRIMER_RIGHT_PENALTY=0.080887
PRIMER_LEFT_SEQUENCE=TGAGGACTTCTCTGGTGATGAA
PRIMER_RIGHT_SEQUENCE=ATCCCAATAACTACACGTCGCT
PRIMER_LEFT=366,22
PRIMER_RIGHT=662,22
PRIMER_LEFT_TM=59.844
PRIMER_RIGHT_TM=59.919
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.2449
PRIMER_LEFT_1_PENALTY=0.061184
PRIMER_RIGHT_1_PENALTY=0.183726
PRIMER_LEFT_1_SEQUENCE=TGGATGGAAGGTTTCCTTTAGA
PRIMER_RIGHT_1_SEQUENCE=GTCGTCACCATCATCATCATCT
PRIMER_LEFT_1=227,22
PRIMER_RIGHT_1=492,22
PRIMER_LEFT_1_TM=59.939
PRIMER_RIGHT_1_TM=59.816
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.4434
PRIMER_LEFT_2_PENALTY=0.061184
PRIMER_RIGHT_2_PENALTY=0.382228
PRIMER_LEFT_2_SEQUENCE=TGGATGGAAGGTTTCCTTTAGA
PRIMER_RIGHT_2_SEQUENCE=CTTCGTCTTCATCCTCGTCTTC
PRIMER_LEFT_2=227,22
PRIMER_RIGHT_2=523,22
PRIMER_LEFT_2_TM=59.939
PRIMER_RIGHT_2_TM=60.382
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=0.4434
PRIMER_LEFT_3_PENALTY=0.061184
PRIMER_RIGHT_3_PENALTY=0.382228
PRIMER_LEFT_3_SEQUENCE=TGGATGGAAGGTTTCCTTTAGA
PRIMER_RIGHT_3_SEQUENCE=TCTTCGTCTTCATCCTCGTCTT
PRIMER_LEFT_3=227,22
PRIMER_RIGHT_3=524,22
PRIMER_LEFT_3_TM=59.939
PRIMER_RIGHT_3_TM=60.382
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=0.4434
PRIMER_LEFT_4_PENALTY=0.061184
PRIMER_RIGHT_4_PENALTY=0.382228
PRIMER_LEFT_4_SEQUENCE=TGGATGGAAGGTTTCCTTTAGA
PRIMER_RIGHT_4_SEQUENCE=TTCTTCGTCTTCATCCTCGTCT
PRIMER_LEFT_4=227,22
PRIMER_RIGHT_4=525,22
PRIMER_LEFT_4_TM=59.939
PRIMER_RIGHT_4_TM=60.382
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_05687 Ps_contig_mira-and-tgicl-ass_franssen_39396
SEQUENCE=AGTNCTCGTCGCCGGCACACACACTCTCTCGAACTCTCTCGGTAGCAGCAACACTATGTCGGCAACGGAGCTTCCACTTCTCGCTTCTGAAAACACCGCCGTGTTTCTATCCTCGGTTGCTGAAGCTTTGGTTCTTCAAACTGTCATTGCTACCGGCAAATCCCTCGCTTACCTTCTCATTGCGACTGGATCTCTTCTTACAGATGTCAGTAATTTGATATCACCAATGGATGGAAGGTTTCCTTTAGACCAGCTGTACAAGGGAAACCATACTTGTGAAGAGAACAAAGATGGAAGTGAAACAGAGGATGATGACGACGATGACGATGACGATGATGTGAATGACGAGGATGATGATGACGATGATGAGGACTTCTCTGGTGATGAAGAGGACGATGAAGCCGACCCTGAGGATGATCCTGTGCCTAACGGTGCAGGAGGAAGTGACGACGATGATGATGATGAGGACGGAGATGATGATGATGGTGACGACGAGGATGATGAAGACGAGGATGAAGACGAAGAAGAGGATGAAGATGAAGAAGTGGCGGCTCTTCAACCACCTTCTAAGAAGAAGAAATAAGTGTTATATTAGTAGGTAAATTGTTTTCTTCCTGCCTGTTGAGCACTTTTTATTTTGTAGCGACGTGTAGTTATTGGGATGCTGTGACATATCATCTATCTTGCCTATGGCCAACATTAAGATTTACAAACACCATCGTTAATATATTATGATGTATCCCTTGTTAATTTATTGGTGTTGCTTGTTGTGATAGTATGATTTTTATTGTTGCCGAGCATTCAAATTTGATGTTAATCCGATACACCTTTTCTCATTAGAGTTTTGAGATTGGACCTTTT
TARGET=473,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2369
PRIMER_LEFT_PENALTY=0.156061
PRIMER_RIGHT_PENALTY=0.080887
PRIMER_LEFT_SEQUENCE=TGAGGACTTCTCTGGTGATGAA
PRIMER_RIGHT_SEQUENCE=ATCCCAATAACTACACGTCGCT
PRIMER_LEFT=366,22
PRIMER_RIGHT=662,22
PRIMER_LEFT_TM=59.844
PRIMER_RIGHT_TM=59.919
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.4434
PRIMER_LEFT_1_PENALTY=0.061184
PRIMER_RIGHT_1_PENALTY=0.382228
PRIMER_LEFT_1_SEQUENCE=TGGATGGAAGGTTTCCTTTAGA
PRIMER_RIGHT_1_SEQUENCE=CTTCGTCTTCATCCTCGTCTTC
PRIMER_LEFT_1=227,22
PRIMER_RIGHT_1=523,22
PRIMER_LEFT_1_TM=59.939
PRIMER_RIGHT_1_TM=60.382
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.4434
PRIMER_LEFT_2_PENALTY=0.061184
PRIMER_RIGHT_2_PENALTY=0.382228
PRIMER_LEFT_2_SEQUENCE=TGGATGGAAGGTTTCCTTTAGA
PRIMER_RIGHT_2_SEQUENCE=TCTTCGTCTTCATCCTCGTCTT
PRIMER_LEFT_2=227,22
PRIMER_RIGHT_2=524,22
PRIMER_LEFT_2_TM=59.939
PRIMER_RIGHT_2_TM=60.382
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=0.4434
PRIMER_LEFT_3_PENALTY=0.061184
PRIMER_RIGHT_3_PENALTY=0.382228
PRIMER_LEFT_3_SEQUENCE=TGGATGGAAGGTTTCCTTTAGA
PRIMER_RIGHT_3_SEQUENCE=TTCTTCGTCTTCATCCTCGTCT
PRIMER_LEFT_3=227,22
PRIMER_RIGHT_3=525,22
PRIMER_LEFT_3_TM=59.939
PRIMER_RIGHT_3_TM=60.382
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.4434
PRIMER_LEFT_4_PENALTY=0.061184
PRIMER_RIGHT_4_PENALTY=0.382228
PRIMER_LEFT_4_SEQUENCE=TGGATGGAAGGTTTCCTTTAGA
PRIMER_RIGHT_4_SEQUENCE=CTTCTTCGTCTTCATCCTCGTC
PRIMER_LEFT_4=227,22
PRIMER_RIGHT_4=526,22
PRIMER_LEFT_4_TM=59.939
PRIMER_RIGHT_4_TM=60.382
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=300
=
PRIMER_SEQUENCE_ID=SSR_05688 Ps_contig_mira-and-tgicl-ass_franssen_39405
SEQUENCE=GGATGAACAARTNNNTNTTGGTTNGCTCTATCATATCTACTTCACGTGTTTCTCATACAATATTAAGAATATATAATATACACATCATTATCAGATATCAGCAAAGTTATAAGCAGTTATTGAACCGATAATTTCAGGGGGAATGTTTGTGATATGCTACAATGTTAATTTTTTTCCTTTCATAAAATAAAATAAAGTATAGCTGAAAACTATCTTGGCCCTTGTGTCTGCTGAAGGTCATAGGGATGATGCAATGTTGCCCCATACGCATATTGTTGTGGATGAGCTTCAATCCCAGCATGGATCTGATGCATGCTATAAGTATCAGGAGGGTATGTGACTCCAACAAACCCCATATCAGGATTGTTTGCATGATATACCGGGCTTGGATTTGCAGCAGCAACAATGGCAGCCCTTGCTCTCTTTTCTGCATTAGCCAGCTCAGCACGTAACTTTTCAACCTCACGGGTCATAATGATCATATTGTTGTCCATTACCCGTTTTTGCTCTAGGTTATTAGCATGTGTCTTCTTCTCAAACTCAATGGCATTCCTTCCCCGATGAATCTCATGGCGCAACGCTTCAATATCACCTTTGATAGCCGGCAAGGACTTCGAATCATTACGAGCCAAATCGAGTTCACTCTGAATGGACTGCAATTGCGACGTAAGCTCCTTCCGCTCGGCCGCAAGCTCACGCACATCATCCCGCACCTGATCAAGGTCAGACTTCATGGCAGAGACAGCGCGTACCTCGGCGTCCATCTTGAGCGACTTCTCATAGATCCGGCGCACCTCCGCGTCTCGTTCGGCTTTAACATCGGCGGCGGCAACGGAGAGCTGACGGTAG
TARGET=182,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05689 Ps_contig_mira-and-tgicl-ass_franssen_39406
SEQUENCE=AGGGGGGGCTGTGGTATAGCATAGCTTANTACACCTCTACTCGAGTCCTTCAAAATAGACAAATGCAATCGCAACTTCTGGTGGGGACCCGACTCTCCATCGCTTCCCACTGCTACCGCCGTTGATTCTACTTACCTCTCCGCCGCCCCTCCGCCGCCGTAGACATCTACTAATCTCAGCGTCCTCCTCTTCAGCTCCGGCATTCAACGGCGGTGGCGACCACTACACCGTACTCGGTGTCGCCCGTTCCGCCGATGTTGTTGATAATCAAACGTGCTTATCGCAACCTCGCCCTTAAGTATCATCCTGATGTTAGCAAGGATTCACATGCTTCTGATTTGTTCAAGAATATCCGTCATGCTTATGAAGTACTATCAAATGAAACAACTAGAATTCAGTATGATCGGGAACTTCAATCCAGTCACAAGCCTTACCAAAATAAATGGAGTTACGGCACTGAATTTGAAGATGATGAGGTAAGAAGCTATAGGCGGGCTTACACAAAGAAGAAAATGCATAGTGAAAGATACTGGGAATATTACAATGTCAACGAGGATTACTACAGCAGCGAAGCAGACGAGGAGGAAGATGAAGGAAATTCAAACGAAGAAAGGGGTTCATTTATTGAAGTTCTTAGATCAGCATTCTTGTCATTATTATTATTCCAGACATTAGGAGCTCGGATCTCCCTTACATTTAGCAGTCTGACAGCAGTATTTGATAACAAATTAGATGCTGGATATAAAGTTGGTTATGTAATAGCATGGATTTTGGGTGGAAGGGGTGGCATAATGTTAACATTGTTCTTGTCATTTTTAAGTTGGATTTTTGGGAAAACCAGTAGTAGTGTGGTTGCATTGTTCATGGTAGCCATGTGGGTTGGCTCTTCCCTTGCAAGTTACGCACCAGTTCCTCAAGGTGCGCTGTTAACGCTTATCTACATGTCCATTAAGCTGCAATCTCATCAAATATAAAGATTCCCCTCTGCTA
TARGET=653,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1983
PRIMER_LEFT_PENALTY=0.068453
PRIMER_RIGHT_PENALTY=0.129818
PRIMER_LEFT_SEQUENCE=AGGATTACTACAGCAGCGAAGC
PRIMER_RIGHT_SEQUENCE=AGATCCGAGCTCCTAATGTCTG
PRIMER_LEFT=552,22
PRIMER_RIGHT=686,22
PRIMER_LEFT_TM=60.068
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=0.2313
PRIMER_LEFT_1_PENALTY=0.101479
PRIMER_RIGHT_1_PENALTY=0.129818
PRIMER_LEFT_1_SEQUENCE=TGAGGTAAGAAGCTATAGGCGG
PRIMER_RIGHT_1_SEQUENCE=AGATCCGAGCTCCTAATGTCTG
PRIMER_LEFT_1=472,22
PRIMER_RIGHT_1=686,22
PRIMER_LEFT_1_TM=59.899
PRIMER_RIGHT_1_TM=59.870
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=12.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=215
PRIMER_PAIR_PENALTY_2=0.2315
PRIMER_LEFT_2_PENALTY=0.101680
PRIMER_RIGHT_2_PENALTY=0.129818
PRIMER_LEFT_2_SEQUENCE=ATGGAGTTACGGCACTGAATTT
PRIMER_RIGHT_2_SEQUENCE=AGATCCGAGCTCCTAATGTCTG
PRIMER_LEFT_2=442,22
PRIMER_RIGHT_2=686,22
PRIMER_LEFT_2_TM=59.898
PRIMER_RIGHT_2_TM=59.870
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=0.2607
PRIMER_LEFT_3_PENALTY=0.130883
PRIMER_RIGHT_3_PENALTY=0.129818
PRIMER_LEFT_3_SEQUENCE=TACGGCACTGAATTTGAAGATG
PRIMER_RIGHT_3_SEQUENCE=AGATCCGAGCTCCTAATGTCTG
PRIMER_LEFT_3=449,22
PRIMER_RIGHT_3=686,22
PRIMER_LEFT_3_TM=60.131
PRIMER_RIGHT_3_TM=59.870
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.2898
PRIMER_LEFT_4_PENALTY=0.160020
PRIMER_RIGHT_4_PENALTY=0.129818
PRIMER_LEFT_4_SEQUENCE=CTATAGGCGGGCTTACACAAAG
PRIMER_RIGHT_4_SEQUENCE=AGATCCGAGCTCCTAATGTCTG
PRIMER_LEFT_4=484,22
PRIMER_RIGHT_4=686,22
PRIMER_LEFT_4_TM=60.160
PRIMER_RIGHT_4_TM=59.870
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_05690 Ps_contig_mira-and-tgicl-ass_franssen_39415
SEQUENCE=CGTATCGAACTTNTAAGGATTATAAAATAACCTGAGCTTTTAGGCTTCTAGCATCAACAAAATCATAAAGATTCACATCCAATAATAGTTCTTTCCACACACAGTAAAACTGTATAAGAATAATCAATGATGAAACCTCTTATTCAATCCACTTCTCAAACTATTCAATCTTTTGAATAGCAAATGAAATGAAATGAATTTGCATATATGGTTTACAATATCCCAGATTTTATTGTGCTCCCTCCAATAAATACTACTTCCAAGGTGTTTGTTGTTTCCAAGGACAAAAATTTGCTACTCTGCATAACTGAATTATCAGTCTAAGCTGATTTTGTAACCTCGTGGATAGCGTTAGCCACATATCCAACGTTGCCCGAATTAATACCAGCCATACTTATACGACCATTACGAGTCATGTAAATGTGAAACTCGCTTGTCAAACGATCAACTTGTTCTGGTGTCATACCGCTGTAGCAGAACATGCCAATCTGATTGGTTATGTGGTTCCATGGCAAAGGAGACCCCAGATTTTCCAAGTTCTCCCGTAGTGCAGTCCTCATTCCAATGATGCGGTCTGCCATAACCTTAACTTCTGTGAGCCATTGCTTCTTCAACTCTGGATCACCAAGGACGGTTGAAACAACAAGTGCTCCATGAAGAGGTGGATTACTGTACATGGGTCTGGAAATCAGCTGCAGCTGACTTTTTACTGCCACAGCTTGTTTCTCATCTTCGCAAAGCAAGCTAAGGCATCCTACTCTCTGGCCATACAGTCCCATATTTTTTGCAAATGATTGAGCAAGTCCTATTGAATGACCATCATCGAGAAAAATCCTGATGGCTTTTGCATCTCTCTCTGGATTGCCACTAGCAAAACCTTGATATGCCATGTCAAACAAAGGGAAATGACCTTTAGCCTTGATTTGGGAAGAAATCTCTCGCCATTGTTCTTCTGAAGGATCTACCCCAGTAGGATTGTGAGCACAAGCATGAAGCAGAAAGAAGGAACCATTGGGAGCATTCTTAATGTCCTCCAGCAGTCCAGAAAAATCCAGTCCTTTAGACTCGGGATGATAGTAACGGAATGTCTTTATGGGCACTCCAGCATCTCTCCAAATGTTATGGTGGTTGGACCAAGTGGGAATTGGTATATATATTTGCGAGTTGGGATGAAAGCGCTGTTGAAATGCAGCAAAAAGTCGACATGCGCCAGTCCCAGATAAAGCCTGAACTGCAGCTATTCTTTTATCCTTGATGAACTCAGAGTTATCTCCATATGCCAGCTTCAGTGATTCTTGAACCATATGTAAGCTTCCACCCATAGGAAGATACTCCATGAATTGGTTTCCTGCAATTCTCTTCTCTGCTTCTCTAACACATCCCAAAACCACTGGCTTTCCGTTGTCATCACGATATGCACCTACTCCGACGTTGATTTTGTTGGGACTCTGATCAGCGAGAAAAGCTTCGGTAACTCCGAGGATTGGATCCTTGGGAGCCTGTTCGATGTTTCTGAACCACGAAGACATGAACCTAGCTCCGGTAACAGAGCTTCCGTTGAGAAATTTTCCGTTGAGCACATTGCGAATAGCCATGAGAAATATGGATACAGAGTAGAGAGGTGGGAGATTGAGTCGAAGTCGAAACTCAACAAGCAAAACGCAAAAAAGCNGGTATGTGTCTTTTTCTTCCCTTCTT
TARGET=182,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0389
PRIMER_LEFT_PENALTY=1.004810
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=CCTGAGCTTTTAGGCTTCTAGC
PRIMER_RIGHT_SEQUENCE=GAGGGAGCACAATAAAATCTGG
PRIMER_LEFT=30,22
PRIMER_RIGHT=243,22
PRIMER_LEFT_TM=58.995
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=214
PRIMER_PAIR_PENALTY_1=1.0389
PRIMER_LEFT_1_PENALTY=1.004810
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=CCTGAGCTTTTAGGCTTCTAGC
PRIMER_RIGHT_1_SEQUENCE=GGAGGGAGCACAATAAAATCTG
PRIMER_LEFT_1=30,22
PRIMER_RIGHT_1=244,22
PRIMER_LEFT_1_TM=58.995
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=215
PRIMER_PAIR_PENALTY_2=1.0911
PRIMER_LEFT_2_PENALTY=1.004810
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=CCTGAGCTTTTAGGCTTCTAGC
PRIMER_RIGHT_2_SEQUENCE=TGTCCTTGGAAACAACAAACAC
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=285,22
PRIMER_LEFT_2_TM=58.995
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=1.2922
PRIMER_LEFT_3_PENALTY=1.004810
PRIMER_RIGHT_3_PENALTY=0.287386
PRIMER_LEFT_3_SEQUENCE=CCTGAGCTTTTAGGCTTCTAGC
PRIMER_RIGHT_3_SEQUENCE=CCTTGGAAACAACAAACACCTT
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=282,22
PRIMER_LEFT_3_TM=58.995
PRIMER_RIGHT_3_TM=60.287
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=253
PRIMER_PAIR_PENALTY_4=1.2975
PRIMER_LEFT_4_PENALTY=1.263361
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=ACCTGAGCTTTTAGGCTTCTAGC
PRIMER_RIGHT_4_SEQUENCE=GAGGGAGCACAATAAAATCTGG
PRIMER_LEFT_4=29,23
PRIMER_RIGHT_4=243,22
PRIMER_LEFT_4_TM=59.737
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_05691 Ps_contig_mira-and-tgicl-ass_franssen_39460
SEQUENCE=ACTTTAAAATTACTATCCCAAAGAATTTARRGGATTTCACAAAAAAAAAATACTCTCTCTTCCTTTCTCAAATACAGAACGCTCTGCAATTTCTCACCTCTTTCTCCATCCGTGCCCTACCGGAGAAGACGGTGGTTGCCGGCCGATGGTGGCGGAGCCACCTTCTTCTCCGGCGAAACCTTAACCAAAACACAAAACCTACACATTAACCCCCCCCCCTATTCTTTGCCAGGGAACACAAATCCGGTCTTAAATCTACCAAAACAAACCCCCTCCGACCGGATCTACAACCATCCAAGAAAGTAAAAGGTTAAATTCCCCGACAGCACAAAACCTAAGTTCAAACTCCTTCTAAATATGAAACCAAAGTCGAAATCAACGTAAATACAAACCAATCTTGAAAACCCTAGCTTTGGAATTGAATTAACGACAGCGGGCATTTTAAAGCAATTAGCAAGGGGATTTTAAAAGGCTCGTCAAGGCGAGCAAGGCGATGCGACGAGTCTAAGCAAAGAAAGAAAAGAAAATATACTTGATCGGAGTCCAGTTACCGGCGACGCTTCCCCACTACGTTTGAGATAAGTCCTCTTTTAATTTTGATTCCTCTTTGTCTTCTTCTTCTCTAGTTCTTCTCTATTATTCTCTTATTCTTCTCCGTTTGCTTATTAATTGTTGTGTTGGCCTGAGCTNATCGTACG
TARGET=611,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05692 Ps_contig_mira-and-tgicl-ass_franssen_39487
SEQUENCE=GTGAATTTTCAACAACTTTAAAAGCTTCATAAATACATTAACACTAAAACACTCACAAATACATAGAAACAGAAAAAATACCAGAAACATGACAGTACTCTCTATTTTGAGGTTTAGTTTTGCATCACCTCAGATACATCCATGGCTATATTATATCTATCTATCTGAAGTACTCTTCATTGCATCACCATGGAACTTTTGCTCATACATATTATCTATAATCTATCTATCAGCTTTTTCTTCTGCTGTCTTGGAAATTAGCAAGATTTATAATGGTGAATGGTGATCACAATACACAGATACCTAGCTAGCTACCTTAGCTTCTCCTGATTCTCACTTCTTCTCGTTAACTGTTCCAGCACATCTCATCTCACCTACTAACTTCAATCTCACTGCCATAACCACTTCACCAAAGTTTTAAAAAACTGTCCCGTTATCACAAAAACGAGTGCGACAGATACCAATACCAT
TARGET=154,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0380
PRIMER_LEFT_PENALTY=0.037936
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=TTGCATCACCTCAGATACATCC
PRIMER_RIGHT_SEQUENCE=GTGGTTATGGCAGTGAGATTGA
PRIMER_LEFT=119,22
PRIMER_RIGHT=404,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.0558
PRIMER_LEFT_1_PENALTY=0.037936
PRIMER_RIGHT_1_PENALTY=0.017846
PRIMER_LEFT_1_SEQUENCE=TTGCATCACCTCAGATACATCC
PRIMER_RIGHT_1_SEQUENCE=TGCTAATTTCCAAGACAGCAGA
PRIMER_LEFT_1=119,22
PRIMER_RIGHT_1=262,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=60.018
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=0.1638
PRIMER_LEFT_2_PENALTY=0.037936
PRIMER_RIGHT_2_PENALTY=0.125826
PRIMER_LEFT_2_SEQUENCE=TTGCATCACCTCAGATACATCC
PRIMER_RIGHT_2_SEQUENCE=GAGATGAGATGTGCTGGAACAG
PRIMER_LEFT_2=119,22
PRIMER_RIGHT_2=371,22
PRIMER_LEFT_2_TM=59.962
PRIMER_RIGHT_2_TM=59.874
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.2136
PRIMER_LEFT_3_PENALTY=0.037936
PRIMER_RIGHT_3_PENALTY=0.175676
PRIMER_LEFT_3_SEQUENCE=TTGCATCACCTCAGATACATCC
PRIMER_RIGHT_3_SEQUENCE=GATGTGCTGGAACAGTTAACGA
PRIMER_LEFT_3=119,22
PRIMER_RIGHT_3=364,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=60.176
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=0.3147
PRIMER_LEFT_4_PENALTY=0.037936
PRIMER_RIGHT_4_PENALTY=0.276812
PRIMER_LEFT_4_SEQUENCE=TTGCATCACCTCAGATACATCC
PRIMER_RIGHT_4_SEQUENCE=TAGGTGAGATGAGATGTGCTGG
PRIMER_LEFT_4=119,22
PRIMER_RIGHT_4=376,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=60.277
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_05693 Ps_contig_mira-and-tgicl-ass_franssen_39493
SEQUENCE=TTAGAAGAGAAAAGAACTAAACTTTGGTGCAATCTTTTAGATTTAGCATGATACAACAAGATCCATAATCCAATTAACATTTGCTATGAGATTCAAATCACCAAAATGGATAACTTTGAACATACATACATGCACAGAACAAGGAAAGTTCCCTCCACTTAACTCCTCTGTTGGGCAAATTGGTTATCACATTTTCAACCACTTTGGCTTAACAATCTTTTTCTCGGCCGGTTTTCGTTCTTCAACGGCAGGAGCTTGTGCAACAGGCTCAGCAGACACCGGTTCAGACTGTAATGGCTCTGACGGCTCACCTTGATCTATAGCACCTTTCAAAGACATCACTTCTTCTAGTAGGTAAGGGCCGTCTTCGCTGCTATTTCCTTTTGAAACATCATACGAAAAGTACACAATGGCACCAGGACAAAATCCGACAGTATAGAAGTCCTGAGAAAAGTCTTTGATCAGCTTCTTCGGTGGAGTAGTATATAAATAAAAGGGCTTCTCTGGTTGTGCAATCACTTTGTTGAGGAGATCCATTAAACTTTGGATGGTTTCTGAGGGATGAAAGGTAGCTTCCAATGTATGATTATCCGGGAAGCGAACTCTAATCACAGCCTTTGTTATTTTTGACCTGCGAGCTGCCTCATCTGCTTCTCGGAGTTTTCGAGTCTTTAGATATTTATCTTCTTTTTTAGTACCCAAGAGTTTGTAATAATCTGCAGCAGTGAACTCGTAGAACTCGTCGGTCTCCTCTTCTTTGTTTGCTGCATCATTTTGTGTTGGAGAAAGTGAAAATGTTTCAAAAACACGAATCTCTCGTCCAAATCTTTCACTCATCTCTCTAATTGTTGCCATGGAATAAAAGTGGTTGATTCTTCTTCTTTTGGATGGTTGAGGAAAAGAAGGATCCACTACCATGAGAGAATACAGTAAGAGCAAGTCTTGTTCTTTCCAATCGAATCGTCAGAAACTTTCGTTTTGGGTATAATCTATTTGTTCTGTTCAAATATTTNCNYGCARTTTTCGTTCTTT
TARGET=120,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05694 Ps_contig_mira-and-tgicl-ass_franssen_39509
SEQUENCE=CCCAAAACCATCGCTAGTTTTCTCTACAATCTTTCCACAATTCAAACTTCCTCTCATAAACTCTCTAAACTCCACGATTTTCTCTCCAGATTGGAACAAGAACTCAATAAGATCCACGCATTCAAACGCGAACTACCTCTCTCCATGCTCCTCTTAAACGATGCAATCTTAATTTTGAAGGAGGAATTGGAGAAATGCAGACGTGAGAATTGTGTGCCGGTTTTGGAAGAGTTTATTCCGTTGAAGAAAGAGATTGATCATAGTGAGGAAAATCATGATATTGATAGAGATAAGAATAATGAATGCAGAGAATAAGCATGCGTTTGAGGGTTTGGAGTCTTTGGTTGACGTGGCAGGTGGACATGGTGGTAGTTCCAACAATGGTAACTGACTATGTCACCATGCATGCATGTACTATGTGTAATCATGAATAAATACTGTTGGGTTTCCAACAAAAGATGTATRTNGATAAATCACTATGTTATCATGTGTAATATATCAAATATCATCCTTGTTGTTAGGAACATTGCCTTCTTTTATATAATCCTATTCTGCTGATTTGTCTAG
TARGET=401,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05695 Ps_contig_mira-and-tgicl-ass_franssen_39518
SEQUENCE=ATTGACTCATACGCGGGGAACATTAGTCTTCTCTAATATAGTCTTCTCCGCCTCTTCATCTTCTTCTTCATCACAAACAAAACACAATCTCTTTCTCCGAACCAAACCAAACCAAAAACCATGGACAGAGAACAAGAAGACATGCAATTCCTCGGTTTCTTCGGTTGCTACAAACAATCCTTCAAAATCATATTCTCATGGAGAAAAATCTTCACACAAATCACGTTAACTCTCATCCTCCCTCTCTCCTTCATCTTCCTCTTCCACATAGAAGTCTCCAACCTCATCTTCAACAAAATCAAACACACCGAAGAACAAATCGACCAAACCCAACAAGGCACACCGAAATACGATAAACTCACCGACATAGTTTCATCACAGTTCATCACCCTTTTACTTTTCAAATTATTCTACTTCACTTTCCTCTTAATCTTTTCTCTTCTCTCTACATCAGCTATTGTCTACACGACAGCTTCCATCTTCACATCCAAGGATCTCTCCTTCAAAAAAATCATCAAAATTGTTCCAAAGGTTTGGAAGAGACTCATGCTCACTTTCTTATGTGCCTATGCTGTTTTCTTCGCTTACAATTTTCTAACCTTCTTGGTTATAATCTTGTTAGTAGTTACACTTGGTGTAAAAAATGGAGGTGCTGTTTTGCTTATTTTCTTGATAATCTTCTATTTTATTGGATTTGTTTACTTAACTTTGATATGGCAACTAGCTAGTGTTGTGACAGTGTTGGAGGATTTGTATGGATTTAAAGCTATGGTGAAGAGCAAGGAATTGATAAAGGGGAAAATGGGTTTGTCAATATTTATATTTTTGGTGCTAAACGTTTCGTTTTTCTTGATAAGGTTTGTTTTTAAGGTAGTGGTTGTGAATGGAACATGGTGTTTTGGTTACGTGGACAGAACAGGATATGGGATACTATGTTTCTTGTTGCTGTCTTGTTTGTTCTTGTTTGGACTTGTTTTGCAAACTGTGTTGTATTTGGTTTGCAAGAGTTATCATCATGAGAATATTGATAAATCTGCTTTGGCTGATCACCTTGAAGTTTATCTTGGAGAGTATGTGCCATTGACAGCTAAAGATGTTCAGCTGGAGAATTATCGGATTTGAGTAGGAATTCTAATGGAACTTGATGATGTAATGAAACATTAATATATGTGCAAGTTTATTGATTATATGTTTTGTAATTTGTTGCAAGAAATTGATATACTTTTGTAGTAGAAAAAAAAG
TARGET=100,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0585
PRIMER_LEFT_PENALTY=0.016950
PRIMER_RIGHT_PENALTY=0.041553
PRIMER_LEFT_SEQUENCE=TAGTCTTCTCCGCCTCTTCATC
PRIMER_RIGHT_SEQUENCE=GTTTGTAGCAACCGAAGAAACC
PRIMER_LEFT=38,22
PRIMER_RIGHT=174,22
PRIMER_LEFT_TM=59.983
PRIMER_RIGHT_TM=60.042
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=0.3789
PRIMER_LEFT_1_PENALTY=0.016950
PRIMER_RIGHT_1_PENALTY=0.361969
PRIMER_LEFT_1_SEQUENCE=TAGTCTTCTCCGCCTCTTCATC
PRIMER_RIGHT_1_SEQUENCE=AGGGAGGATGAGAGTTAACGTG
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=242,22
PRIMER_LEFT_1_TM=59.983
PRIMER_RIGHT_1_TM=59.638
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.5002
PRIMER_LEFT_2_PENALTY=0.016950
PRIMER_RIGHT_2_PENALTY=0.483253
PRIMER_LEFT_2_SEQUENCE=TAGTCTTCTCCGCCTCTTCATC
PRIMER_RIGHT_2_SEQUENCE=GATTTGTTCTTCGGTGTGTTTG
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=320,22
PRIMER_LEFT_2_TM=59.983
PRIMER_RIGHT_2_TM=59.517
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.7046
PRIMER_LEFT_3_PENALTY=0.016950
PRIMER_RIGHT_3_PENALTY=0.687611
PRIMER_LEFT_3_SEQUENCE=TAGTCTTCTCCGCCTCTTCATC
PRIMER_RIGHT_3_SEQUENCE=TGTTTGTAGCAACCGAAGAAAC
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=175,22
PRIMER_LEFT_3_TM=59.983
PRIMER_RIGHT_3_TM=59.312
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=0.7461
PRIMER_LEFT_4_PENALTY=0.016950
PRIMER_RIGHT_4_PENALTY=0.729140
PRIMER_LEFT_4_SEQUENCE=TAGTCTTCTCCGCCTCTTCATC
PRIMER_RIGHT_4_SEQUENCE=GATTGTTTGTAGCAACCGAAGA
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=59.983
PRIMER_RIGHT_4_TM=59.271
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_05696 Ps_contig_mira-and-tgicl-ass_franssen_39522
SEQUENCE=TACTAGAAACAACTAATCTTCATCCGGAATATCTGCACTCAACGAACTAACCGCCTTCTTCGAAGACTTGGATGACTTCTTTTTCTTGCCAGTGAAAGTGATTGGTCCTATATCTTCATCATCCTCTTCTACCTTTTCATTCTTACTCTCATCCTCTCCATCAATCTCATCAAAATTAGCAGATGAAAACAAAGAACCGCCCTTCTTGCTTCCTTTTGAAGACTTCTTCTTCCCAGTAAAGCTAACAACAGGTTCATCCTCATCGCCATCTCCATCACCAGTTAACTCTGATTTCTGTTCATCATCATCATTATCAACTTCCTCCTCATCCCCAAGCAAACTAAAACCAGCCGCAGCAGTAAACACACTTCCACCGCCACTTTTCTTTGGTTTCCCCTTTTTCTTCCCAGCAAACACAATTTCAGGCACATCATCTTCGTCCTCGCCATCAAAATCATCACCATTATCTTTAGCTTGTAAAAATTTAGAGTTGGCTTTTTTACCTTTCTTCTTCCCCTGCTTTTCTTCTGGCTGCGGCAGGGATGGCTCCTCTGAAAGTTCAGTTCCGATGGAGTATTCATCATCGTCGATGACTTGAGCCTTCTTCTTCGATTTCCCACCACCTTGCTGCGGAGGATTCTCGTCATCGCGTACCGATGGTTTCTTCTTCCCCATTTGTCAGATTAGGGTTTTCTCGTGCGTCTAAACAAAGCACGAATAATTGGATCGATAAAAGAGAGATTCGTGTTTTGATAAACGGAGAGAAGGGAAAAACCTGATCGGTGAGAATCAGTGGGTACGAAAATTGGATAGATAGAAAGGGGAAAGTCGATTGGAACTGCAGCGAAAGTGCGGCGGTGAAGGAAGATACAGGAGAGTTAAAACGCACAAACCTCCCCCGNGTACTCTAGAACGTTAAGAATACCACTGCTT
TARGET=299,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0057
PRIMER_LEFT_PENALTY=0.005674
PRIMER_RIGHT_PENALTY=0.000037
PRIMER_LEFT_SEQUENCE=CCTTCTTGCTTCCTTTTGAAGA
PRIMER_RIGHT_SEQUENCE=AGATGATGTGCCTGAAATTGTG
PRIMER_LEFT=200,22
PRIMER_RIGHT=435,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.000
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=236
PRIMER_PAIR_PENALTY_1=0.1400
PRIMER_LEFT_1_PENALTY=0.005674
PRIMER_RIGHT_1_PENALTY=0.134318
PRIMER_LEFT_1_SEQUENCE=CCTTCTTGCTTCCTTTTGAAGA
PRIMER_RIGHT_1_SEQUENCE=GATGATGTGCCTGAAATTGTGT
PRIMER_LEFT_1=200,22
PRIMER_RIGHT_1=434,22
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=59.866
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.1577
PRIMER_LEFT_2_PENALTY=0.005674
PRIMER_RIGHT_2_PENALTY=0.151991
PRIMER_LEFT_2_SEQUENCE=CCTTCTTGCTTCCTTTTGAAGA
PRIMER_RIGHT_2_SEQUENCE=GTGGAAGTGTGTTTACTGCTGC
PRIMER_LEFT_2=200,22
PRIMER_RIGHT_2=373,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=59.848
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.3035
PRIMER_LEFT_3_PENALTY=0.005674
PRIMER_RIGHT_3_PENALTY=0.297793
PRIMER_LEFT_3_SEQUENCE=CCTTCTTGCTTCCTTTTGAAGA
PRIMER_RIGHT_3_SEQUENCE=GGTGGAAGTGTGTTTACTGCTG
PRIMER_LEFT_3=200,22
PRIMER_RIGHT_3=374,22
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=59.702
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.3128
PRIMER_LEFT_4_PENALTY=0.005674
PRIMER_RIGHT_4_PENALTY=0.307096
PRIMER_LEFT_4_SEQUENCE=CCTTCTTGCTTCCTTTTGAAGA
PRIMER_RIGHT_4_SEQUENCE=GAAGAAAAAGGGGAAACCAAAG
PRIMER_LEFT_4=200,22
PRIMER_RIGHT_4=405,22
PRIMER_LEFT_4_TM=59.994
PRIMER_RIGHT_4_TM=60.307
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=206
=
PRIMER_SEQUENCE_ID=SSR_05697 Ps_contig_mira-and-tgicl-ass_franssen_39524
SEQUENCE=GCGATTGGGTGCGGCGGTTCCAACTATTGAGCTCTTTTTGCAGAATGAAAATACAGTTTGGAGGATATTTGGTGCGAATTCGATGGTGAATATAAATGATGAGGTATTGTGTCTTGGATTTGTGAAAGGTGGGCAGAATTTGATGACNTCGATNGTGCTTGGTGGGTATCAGTTGGAGGATAATCTTGTGCAATTTGATTTGGCTGCATCTAGATTGGGTTTTAGCTCTTTGCTCTTTGGACGACAAACCGGATGTTTTAACTTCAACTTTACCTCCAATGCATAGATTTGTTTACCCTTTTATACACATGGTTGGTTTTATCTCTCTCTATATATATAGTATGTATCTAAGATTGAATAAATTGTTCTACTTCATGNAYTAATTCTTTTAAATAACCTAGCTAATTAGTTAAAAATTAGGTAATTTCTATTTAGAAATTAAGAGAAGAGTGGA
TARGET=330,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05698 Ps_contig_mira-and-tgicl-ass_franssen_39530
SEQUENCE=GAAGTTACGTCAAAATACTAATCTAATCAAATAAAAACAACAAACCAACGGCGTTACCCTTAAACGACCGATCATCACAACATTCACTCTCNCTCTCTCTCAATTTCTGTGGCGGCTCTCTCTCTACTAATTCTCTTCTCAGATTGAGATTGAGATCCAATTTTCCCTTCTTCTTCATCTCTTTTTGGGTTGGGTTCGCCATGGTTGCCCAACACCCGCAGCTTCAGATTCAGACCCAACAACCTACACCTTCTCCTCAAGATGAAGCCTTGAAGAAGAAACACCGTATTGTGTATACTTTCTTGCTTCTCCCTTGAC
TARGET=116,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3184
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.314775
PRIMER_LEFT_SEQUENCE=CTTAAACGACCGATCATCACAA
PRIMER_RIGHT_SEQUENCE=AAGGTGTAGGTTGTTGGGTCTG
PRIMER_LEFT=58,22
PRIMER_RIGHT=251,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.315
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=194
PRIMER_PAIR_PENALTY_1=0.3858
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.382228
PRIMER_LEFT_1_SEQUENCE=CTTAAACGACCGATCATCACAA
PRIMER_RIGHT_1_SEQUENCE=GCTTCATCTTGAGGAGAAGGTG
PRIMER_LEFT_1=58,22
PRIMER_RIGHT_1=267,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=60.382
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.4582
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.454655
PRIMER_LEFT_2_SEQUENCE=CTTAAACGACCGATCATCACAA
PRIMER_RIGHT_2_SEQUENCE=CCAACCCAAAAAGAGATGAAGA
PRIMER_LEFT_2=58,22
PRIMER_RIGHT_2=192,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=60.455
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=135
PRIMER_PAIR_PENALTY_3=0.4616
PRIMER_LEFT_3_PENALTY=0.003589
PRIMER_RIGHT_3_PENALTY=0.458000
PRIMER_LEFT_3_SEQUENCE=CTTAAACGACCGATCATCACAA
PRIMER_RIGHT_3_SEQUENCE=AGGAGAAGGTGTAGGTTGTTGG
PRIMER_LEFT_3=58,22
PRIMER_RIGHT_3=256,22
PRIMER_LEFT_3_TM=59.996
PRIMER_RIGHT_3_TM=59.542
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=199
PRIMER_PAIR_PENALTY_4=0.5151
PRIMER_LEFT_4_PENALTY=0.132857
PRIMER_RIGHT_4_PENALTY=0.382228
PRIMER_LEFT_4_SEQUENCE=TTAAACGACCGATCATCACAAC
PRIMER_RIGHT_4_SEQUENCE=GCTTCATCTTGAGGAGAAGGTG
PRIMER_LEFT_4=59,22
PRIMER_RIGHT_4=267,22
PRIMER_LEFT_4_TM=59.867
PRIMER_RIGHT_4_TM=60.382
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_05699 Ps_contig_mira-and-tgicl-ass_franssen_39547
SEQUENCE=AAGCAGTGGTATCAAACGCCACGAGTACGCGNCGGGAGTCCACTCAACAATTAAGTCTTTCTCTCTCTACCGACCCTTTTTTCTTCCTCTCTCTCTCTCTCTTCAGCTTCTACATTCCTTCCTTACTTTAAAGCAAATTCTAGAGAGAGAGAAAGCGAGAGAGTGTGTATTTCGAGTCTTTTCACAATGGCAGTCTTCTATAGAAATTGGTTTCTCTAATTTTGTTTGTTTACTTTTGTGCTGTCTCGTTAGAAGAAGAAGCTATGGTCTTACACA
TARGET=87,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6623
PRIMER_LEFT_PENALTY=1.021538
PRIMER_RIGHT_PENALTY=0.640802
PRIMER_LEFT_SEQUENCE=CGGGAGTCCACTCAACAATTA
PRIMER_RIGHT_SEQUENCE=GAAGACTGCCATTGTGAAAAGA
PRIMER_LEFT=32,21
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.978
PRIMER_RIGHT_TM=59.359
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=1.7388
PRIMER_LEFT_1_PENALTY=1.097964
PRIMER_RIGHT_1_PENALTY=0.640802
PRIMER_LEFT_1_SEQUENCE=TCTTTCTCTCTCTACCGACCCTT
PRIMER_RIGHT_1_SEQUENCE=GAAGACTGCCATTGTGAAAAGA
PRIMER_LEFT_1=55,23
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=59.902
PRIMER_RIGHT_1_TM=59.359
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=1.8567
PRIMER_LEFT_2_PENALTY=1.215927
PRIMER_RIGHT_2_PENALTY=0.640802
PRIMER_LEFT_2_SEQUENCE=TTCTCTCTCTACCGACCCTTTTT
PRIMER_RIGHT_2_SEQUENCE=GAAGACTGCCATTGTGAAAAGA
PRIMER_LEFT_2=58,23
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=59.784
PRIMER_RIGHT_2_TM=59.359
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=1.8724
PRIMER_LEFT_3_PENALTY=1.021538
PRIMER_RIGHT_3_PENALTY=0.850828
PRIMER_LEFT_3_SEQUENCE=CGGGAGTCCACTCAACAATTA
PRIMER_RIGHT_3_SEQUENCE=TCGAAATACACACTCTCTCGCT
PRIMER_LEFT_3=32,21
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=59.978
PRIMER_RIGHT_3_TM=59.149
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=1.8724
PRIMER_LEFT_4_PENALTY=1.021538
PRIMER_RIGHT_4_PENALTY=0.850828
PRIMER_LEFT_4_SEQUENCE=CGGGAGTCCACTCAACAATTA
PRIMER_RIGHT_4_SEQUENCE=CTCGAAATACACACTCTCTCGC
PRIMER_LEFT_4=32,21
PRIMER_RIGHT_4=175,22
PRIMER_LEFT_4_TM=59.978
PRIMER_RIGHT_4_TM=59.149
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_05700 Ps_contig_mira-and-tgicl-ass_franssen_39547
SEQUENCE=AAGCAGTGGTATCAAACGCCACGAGTACGCGNCGGGAGTCCACTCAACAATTAAGTCTTTCTCTCTCTACCGACCCTTTTTTCTTCCTCTCTCTCTCTCTCTTCAGCTTCTACATTCCTTCCTTACTTTAAAGCAAATTCTAGAGAGAGAGAAAGCGAGAGAGTGTGTATTTCGAGTCTTTTCACAATGGCAGTCTTCTATAGAAATTGGTTTCTCTAATTTTGTTTGTTTACTTTTGTGCTGTCTCGTTAGAAGAAGAAGCTATGGTCTTACACA
TARGET=142,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6623
PRIMER_LEFT_PENALTY=1.021538
PRIMER_RIGHT_PENALTY=0.640802
PRIMER_LEFT_SEQUENCE=CGGGAGTCCACTCAACAATTA
PRIMER_RIGHT_SEQUENCE=GAAGACTGCCATTGTGAAAAGA
PRIMER_LEFT=32,21
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.978
PRIMER_RIGHT_TM=59.359
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=1.7388
PRIMER_LEFT_1_PENALTY=1.097964
PRIMER_RIGHT_1_PENALTY=0.640802
PRIMER_LEFT_1_SEQUENCE=TCTTTCTCTCTCTACCGACCCTT
PRIMER_RIGHT_1_SEQUENCE=GAAGACTGCCATTGTGAAAAGA
PRIMER_LEFT_1=55,23
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=59.902
PRIMER_RIGHT_1_TM=59.359
PRIMER_LEFT_1_GC_PERCENT=47.826
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=1.8567
PRIMER_LEFT_2_PENALTY=1.215927
PRIMER_RIGHT_2_PENALTY=0.640802
PRIMER_LEFT_2_SEQUENCE=TTCTCTCTCTACCGACCCTTTTT
PRIMER_RIGHT_2_SEQUENCE=GAAGACTGCCATTGTGAAAAGA
PRIMER_LEFT_2=58,23
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=59.784
PRIMER_RIGHT_2_TM=59.359
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=1.8724
PRIMER_LEFT_3_PENALTY=1.021538
PRIMER_RIGHT_3_PENALTY=0.850828
PRIMER_LEFT_3_SEQUENCE=CGGGAGTCCACTCAACAATTA
PRIMER_RIGHT_3_SEQUENCE=TCGAAATACACACTCTCTCGCT
PRIMER_LEFT_3=32,21
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=59.978
PRIMER_RIGHT_3_TM=59.149
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=1.8724
PRIMER_LEFT_4_PENALTY=1.021538
PRIMER_RIGHT_4_PENALTY=0.850828
PRIMER_LEFT_4_SEQUENCE=CGGGAGTCCACTCAACAATTA
PRIMER_RIGHT_4_SEQUENCE=CTCGAAATACACACTCTCTCGC
PRIMER_LEFT_4=32,21
PRIMER_RIGHT_4=175,22
PRIMER_LEFT_4_TM=59.978
PRIMER_RIGHT_4_TM=59.149
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_05701 Ps_contig_mira-and-tgicl-ass_franssen_39558
SEQUENCE=TCCACTCACCAACAACAACACCATTTTCACACCAAAACAGAGAAGAGAGAGAGAGAGAGCTTCACATGGGTCTCTGTTCTTCCTTTCTCTCTTCCACCTCTGAACCTCCCCAACCATCTTCTTCCTTCAACAGACCTCTTTCCTCAGGTTCAACAAGCACTGTTGGATTCTCTGCAACAACAAGCAGTGCAGGAAGGAGTCAAATCTCCGAAATAGCAAGTGGAAGCATCGATAGTGTTACTGAAGGGTCTCTTCGTGTACCTTCTTCCCCAGATGGTCAAATTCTTGAAAGGCCTAATTTGAAGGTTTTTAGTTACGCTGACTTGAAATCTGCTACCAGAAATTTCAAACCAGATACATTACTCGGTGAAGGTGGTTTTGGAAAAGTTTATAAAGGTTGGTTGGTTGAGAAAACTCTTGCTCCTGCTAAAGCTGGCTCTGGAATGATTGTTGCTATAAAAAAATTGAACTCCGAAAGCACTCAAGGTTTTCAAGAATGGCAGNCANAAGTYNNNTTTTYARGAAGGCTTTCACACCCAAA
TARGET=44,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05702 Ps_contig_mira-and-tgicl-ass_franssen_39558
SEQUENCE=TCCACTCACCAACAACAACACCATTTTCACACCAAAACAGAGAAGAGAGAGAGAGAGAGCTTCACATGGGTCTCTGTTCTTCCTTTCTCTCTTCCACCTCTGAACCTCCCCAACCATCTTCTTCCTTCAACAGACCTCTTTCCTCAGGTTCAACAAGCACTGTTGGATTCTCTGCAACAACAAGCAGTGCAGGAAGGAGTCAAATCTCCGAAATAGCAAGTGGAAGCATCGATAGTGTTACTGAAGGGTCTCTTCGTGTACCTTCTTCCCCAGATGGTCAAATTCTTGAAAGGCCTAATTTGAAGGTTTTTAGTTACGCTGACTTGAAATCTGCTACCAGAAATTTCAAACCAGATACATTACTCGGTGAAGGTGGTTTTGGAAAAGTTTATAAAGGTTGGTTGGTTGAGAAAACTCTTGCTCCTGCTAAAGCTGGCTCTGGAATGATTGTTGCTATAAAAAAATTGAACTCCGAAAGCACTCAAGGTTTTCAAGAATGGCAGNCANAAGTYNNNTTTTYARGAAGGCTTTCACACCCAAA
TARGET=396,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05703 Ps_contig_mira-and-tgicl-ass_franssen_39567
SEQUENCE=AATAGAAAAAGTACACATATTCACTTTTTCAGAGAAATAAACTGCTCCTCCCTATATCTATTCATTTCCCGTTTTCCTTCTTCATCTTTCTCGCCTCCCCTAAAATCCTCCTCTCTTCATCATGTCCGATCACTCCGCCGATCTTCCTGCCGCCGCCGCCGCTCTTAGTGCCGAAGATCGTGCTGGTCTCGTTAATGCACTCAAAGATAAGCTTCAGCTCTTGGCTGGTGAGCATGTGGACATCTTGGAAACACTGTCATCAAAAGTCAAGCAACGTGTTGAGGTTCTTAAAGTTTTGCAGAGCGAACATGATGAGCTGGAGACTAAGTTCTTAGAGGAGCGAGCTCAGTTGGAAGCTAAATACCAGAAGCTTTATGCACCTACTTTATACTAAGAGNTATGT
TARGET=149,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0466
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.012470
PRIMER_LEFT_SEQUENCE=TTTCCTTCTTCATCTTTCTCGC
PRIMER_RIGHT_SEQUENCE=GACAGTGTTTCCAAGATGTCCA
PRIMER_LEFT=72,22
PRIMER_RIGHT=257,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=186
PRIMER_PAIR_PENALTY_1=0.0466
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.012470
PRIMER_LEFT_1_SEQUENCE=TTTCCTTCTTCATCTTTCTCGC
PRIMER_RIGHT_1_SEQUENCE=TGACAGTGTTTCCAAGATGTCC
PRIMER_LEFT_1=72,22
PRIMER_RIGHT_1=258,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.0930
PRIMER_LEFT_2_PENALTY=0.080568
PRIMER_RIGHT_2_PENALTY=0.012470
PRIMER_LEFT_2_SEQUENCE=TCTTCATCATGTCCGATCACTC
PRIMER_RIGHT_2_SEQUENCE=GACAGTGTTTCCAAGATGTCCA
PRIMER_LEFT_2=113,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=60.081
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=0.0930
PRIMER_LEFT_3_PENALTY=0.080568
PRIMER_RIGHT_3_PENALTY=0.012470
PRIMER_LEFT_3_SEQUENCE=TCTCTTCATCATGTCCGATCAC
PRIMER_RIGHT_3_SEQUENCE=GACAGTGTTTCCAAGATGTCCA
PRIMER_LEFT_3=111,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=60.081
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=0.0930
PRIMER_LEFT_4_PENALTY=0.080568
PRIMER_RIGHT_4_PENALTY=0.012470
PRIMER_LEFT_4_SEQUENCE=TCTCTTCATCATGTCCGATCAC
PRIMER_RIGHT_4_SEQUENCE=TGACAGTGTTTCCAAGATGTCC
PRIMER_LEFT_4=111,22
PRIMER_RIGHT_4=258,22
PRIMER_LEFT_4_TM=60.081
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_05704 Ps_contig_mira-and-tgicl-ass_franssen_39568
SEQUENCE=AACTCTGATGGTGTTTGTCAGGATGAAGAACCTGGTGGTGGAGGAGGAGGAGGAGAAGGATTAGATGGTCGCGAAAATGTATATGAGAATTGTGRAGTCAATGGTGTGGTTAAAGAGAAGAAGGGCTGGTTTGGATGGAACAAGAAAAGCTTGAAGRATGGTGGCGATGAACATGAGGATTCAAAGAAGTTTTCNAGTGTTGGGCCAAGAAGGTAGCAACCAGAGATCAAGTGATCAGCAAAATCCACAGCCTGAGTTTCAGAAGGAGGATCACGGAGATAAGAAACTACGAAGAAAAGGAAAAGGAATCAAAAACTTGAAGAAGAAAAAGAAGAAAGGTGCAAGTAAGGAGTCTAAGAGTGAGAGTGAGTTCAAAAGGGGTGTAAGACCTGTCTTGTGGCTGACACAAGACTTCCCTTTGAAAATAGATGAGCTTCTTCCTTTACTTGATATCTTAGCAAACAAGGTGAAGGCTATTAGGAGGCTCAGAGAACTTTTGACAACTAAACTTCCTCAAGGAACCTTTCCCGTCAAGGTAGCTATTCCTATAGTCCCTACTATAAGGGTAATTATCACTTTTACAAAATTTGAGGAACATCAGACGACACCAGAGGAATTTTCAACACCACCCACTAGCCCAGTCTACTTCCAGGATCCCAAATCCAAGGAATCAGAAGGGTCGACATCCTGGATTTCGTGGATGAAAGGAAATCGCAGTGTGCTATCTAGTGACTCTGATAGTCATAGTCTCAGTCACAGATATAAGGATGGAGTAGACCCTTTCATTATTCCTTCAGACTACAAATGGGTGGATGCAAGTGAGAGGAAGCGTCGCATGAAGGCAAAAGAGCCAAAAGTAAGAAAAATAAGAAGCAGCAGACAGTCCCTAAAGGAGGAGATGGAGCGCAACAGGGGAGTGATGATGTGGGAGAATTAAACCAATAGTGTGGTATATCTGTATGGCTACTTTTTATAATCGCCGCCAGAGAGAAAGTGTAAAGACAGTATTTTTGCACAGCTGACTATACTTTTCACATTGGATGAACTTGCCACAAATTTACTGGGACCTTTACGCCGCAATGTTTTCGCCTGTTTGTTAGATCGGATCATAAAGAGAGCTGTCATCATATGAAAATTGCTGTATTGTAATATGAAGTGAAAATGAAGCAGGAGTATTGCCTTCAATTAGTTGCCTACTTGGTTTCATTCTACTTAATTTTTACGGTTAAAACGAAAACCTAATTATTTGTATCTGCTATCTTATTGGTTTTAGG
TARGET=40,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05705 Ps_contig_mira-and-tgicl-ass_franssen_39569
SEQUENCE=CATGTGGAGCACTCGACGTAATCCGCATAGTACACTGTAACGGTAGAGTTGAAGAAATCAGTGGCACTATCAAAGCAAGTGAAATCATGAAAGCGTATCCAAAACACGTCCTCAAGAAACCTTCTTCTTCTTCCTCACAAGACGCTGTTGTTCCTAAGATCGTTGTTGGTTCTTTCATGGCACATGAAGGCTGGCACTATGTG
TARGET=121,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4177
PRIMER_LEFT_PENALTY=0.225403
PRIMER_RIGHT_PENALTY=0.192310
PRIMER_LEFT_SEQUENCE=ATCAGTGGCACTATCAAAGCAA
PRIMER_RIGHT_SEQUENCE=ACGATCTTAGGAACAACAGCGT
PRIMER_LEFT=56,22
PRIMER_RIGHT=162,22
PRIMER_LEFT_TM=59.775
PRIMER_RIGHT_TM=60.192
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=107
PRIMER_PAIR_PENALTY_1=0.4177
PRIMER_LEFT_1_PENALTY=0.225403
PRIMER_RIGHT_1_PENALTY=0.192310
PRIMER_LEFT_1_SEQUENCE=AATCAGTGGCACTATCAAAGCA
PRIMER_RIGHT_1_SEQUENCE=ACGATCTTAGGAACAACAGCGT
PRIMER_LEFT_1=55,22
PRIMER_RIGHT_1=162,22
PRIMER_LEFT_1_TM=59.775
PRIMER_RIGHT_1_TM=60.192
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=108
PRIMER_PAIR_PENALTY_2=0.5052
PRIMER_LEFT_2_PENALTY=0.312895
PRIMER_RIGHT_2_PENALTY=0.192310
PRIMER_LEFT_2_SEQUENCE=GTGGCACTATCAAAGCAAGTGA
PRIMER_RIGHT_2_SEQUENCE=ACGATCTTAGGAACAACAGCGT
PRIMER_LEFT_2=60,22
PRIMER_RIGHT_2=162,22
PRIMER_LEFT_2_TM=60.313
PRIMER_RIGHT_2_TM=60.192
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.6295
PRIMER_LEFT_3_PENALTY=0.437208
PRIMER_RIGHT_3_PENALTY=0.192310
PRIMER_LEFT_3_SEQUENCE=CGACGTAATCCGCATAGTACAC
PRIMER_RIGHT_3_SEQUENCE=ACGATCTTAGGAACAACAGCGT
PRIMER_LEFT_3=13,22
PRIMER_RIGHT_3=162,22
PRIMER_LEFT_3_TM=59.563
PRIMER_RIGHT_3_TM=60.192
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=150
PRIMER_PAIR_PENALTY_4=0.6328
PRIMER_LEFT_4_PENALTY=0.440473
PRIMER_RIGHT_4_PENALTY=0.192310
PRIMER_LEFT_4_SEQUENCE=TCAGTGGCACTATCAAAGCAAG
PRIMER_RIGHT_4_SEQUENCE=ACGATCTTAGGAACAACAGCGT
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=162,22
PRIMER_LEFT_4_TM=60.440
PRIMER_RIGHT_4_TM=60.192
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=106
=
PRIMER_SEQUENCE_ID=SSR_05706 Ps_contig_mira-and-tgicl-ass_franssen_39571
SEQUENCE=TCGGGGACTACACACACAACACACGCCCCCAAATAAAACCAAATTATCTTTCTCTTCTCTTCTCTACTAAAATCAACATCACTTCCACTCTTCCCCTTTCTTCTTGAATTCTTCAATTCCTCACAAACCCAATTCAATCAAATAATTTCCCAATTTCCACAAAACCCTAACCCTATGGAACTTTCTCGTCTCTCACTTTCCGACAACTACTTCTTCTTATCCTCGCCAATTCATCGTTCTTCTTGGACTCGCCCGCTTCCCGCGTTGCCCGCATTCTTCACCACCCGCCGCCGCCGGAGAGGTTTATGCTCCGCCTCCAACTCCGACACTCTAGTCGCCGCTGCCGCCAAGAAGCACGGAGAGGATGACACTGACTTGAAGACGTGGATGCATAAGAATGGGCTTCCGCCTTGTAAGGTTGTTCTCAAGGATAAACCTTCGCTTGATGATAGTCTTAAGCCTATACACTATGTTGCTGCTAGCGAAGATCTTCAGAAGGGGGATGTTGCATTCTCGATTCCCAGTTCGTTGGTTGTCACTCTCGAGCGTGTATTGGGAAACGAGACTATCGCTGAGCTATTGACCACAAACAAGTTTTCGGAATTGGCATGCTTGGCCTTATATCTGATGTATGAGAAAAAACAGGGGAAGAAATCTTTCTGGTATCCATACATTAGGGAGCTTGATCGGCAACGAGGCAGGGGACAGCTGGCAGTGGAATCACCTCTTCTATGGTCAGAATCTGAGCTGGCATACCTTGAAGGAAGCCCATTAAAGGATGAAATTGTTAAAAGGATTGAAGGAATAAGAAAAGAGTATAATGAACTAGACTTAGTCTGGTTTATGTCTGGTTCTTTGTTTCAGCAATACCCATATGACATTCCCACCGAGGCTTTTCCATTTGAAATTTTCAAACAAGCTTTTGCAGCAGTTCAGTCATGTGTTGTGCATTTACAGAATGTTAGTTTAGCTCGGAGATTTGCTCTGGTTCCCCTGGGACCTCCTTTGTTAGCCTACTGTAGCAATTGCAGGGCGATGTTATCTGCGGTTGATGGTGCTGTCGAGCTTGTGGTTGATCGCCCATATAAAGCCGGGGACCCAATTGTTGTGTGGTGTGGCCCACAGCCTAACACAAAGTTACTTACAAACTATGGTTTTGTTGATGATGACAATTCCAATGATCGTCTCATAGTTGAGGCGGCTTTGAGTACCGAAGATCCACAATATCAAGAAAAAAGAATGGTTGCTCAAAGAAATGGAAAATTATCAATTCAAACTTTTAATGTGTATACTGGGAAGGAAAGGGAATCTGTCTTAGATATGATTCCGTATCTGCGTTTAGGCTATGTTTCAGATCCTTCTGAGATGCAATCTGTCATCTCCTCTGAAGGTCCAGTTTGTCCTATGAGCCCATGTATGGAGCGGGCCGTGCTGGACCAGCTTGCTGATTATTTCAATTCCCGGTTGGCTGCCTACCCTACAACCTTGGCTGAAGATGAATCTATGCTGGCAGATGGTAGTTTGAATCCAAAGAGGCGAGTTGCTACTCAACTTGTAAGGCTAGAAAAGAAAATGCTTCATGCATGTTTGCAGGCAATCACGGATTTCATAAACCAGCTACCAGACCATAGTGTATCGCCATGCCCTGCTCCTTATGCACCTTCATTAAAATAAAAGAATAACACCATGATTGCTGTACTTAGAACAATGATGCTATGCCTTTCCAAGTCCAGATTAAAAGGGATAGGTTCAAGTATTTTCTGCAGGCCGCATCAAGTTGGGGAGCATCATTGAAGCACTCAACTCCCTAGAAAATGTCGAACTGTGGTCGTGCAAATGATTCTAAATTTGAGTGTTGAAATGAAAGTAATCTTTGTAAATGGAAATATGTATCACAAATTGTAATTCATTTGTGTATATATTTGTATGCAGTTCTCGGGATTATATTTGATCTATTTTTTTTACTTTTGTTACTTTGTAGCCTACCTCTGTGTGGTCACTTNAANTTAGTTACTAAATANNTCAAATGTTATTAAAAGG
TARGET=50,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2576
PRIMER_LEFT_PENALTY=0.242681
PRIMER_RIGHT_PENALTY=0.014945
PRIMER_LEFT_SEQUENCE=GGGGACTACACACACAACACAC
PRIMER_RIGHT_SEQUENCE=AGGGTTAGGGTTTTGTGGAAAT
PRIMER_LEFT=2,22
PRIMER_RIGHT=173,22
PRIMER_LEFT_TM=60.243
PRIMER_RIGHT_TM=59.985
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.3849
PRIMER_LEFT_1_PENALTY=0.242681
PRIMER_RIGHT_1_PENALTY=0.142170
PRIMER_LEFT_1_SEQUENCE=GGGGACTACACACACAACACAC
PRIMER_RIGHT_1_SEQUENCE=TCAAGAAGAAAGGGGAAGAGTG
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=106,22
PRIMER_LEFT_1_TM=60.243
PRIMER_RIGHT_1_TM=59.858
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=105
PRIMER_PAIR_PENALTY_2=0.4959
PRIMER_LEFT_2_PENALTY=0.242681
PRIMER_RIGHT_2_PENALTY=0.253194
PRIMER_LEFT_2_SEQUENCE=GGGGACTACACACACAACACAC
PRIMER_RIGHT_2_SEQUENCE=TAGGGTTAGGGTTTTGTGGAAA
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=174,22
PRIMER_LEFT_2_TM=60.243
PRIMER_RIGHT_2_TM=59.747
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.5420
PRIMER_LEFT_3_PENALTY=0.242681
PRIMER_RIGHT_3_PENALTY=0.299343
PRIMER_LEFT_3_SEQUENCE=GGGGACTACACACACAACACAC
PRIMER_RIGHT_3_SEQUENCE=GAATTGGCGAGGATAAGAAGAA
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=231,22
PRIMER_LEFT_3_TM=60.243
PRIMER_RIGHT_3_TM=59.701
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=0.5748
PRIMER_LEFT_4_PENALTY=0.242681
PRIMER_RIGHT_4_PENALTY=0.332137
PRIMER_LEFT_4_SEQUENCE=GGGGACTACACACACAACACAC
PRIMER_RIGHT_4_SEQUENCE=GGGTTTGTGAGGAATTGAAGAA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=129,22
PRIMER_LEFT_4_TM=60.243
PRIMER_RIGHT_4_TM=60.332
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=128
=
PRIMER_SEQUENCE_ID=SSR_05707 Ps_contig_mira-and-tgicl-ass_franssen_39571
SEQUENCE=TCGGGGACTACACACACAACACACGCCCCCAAATAAAACCAAATTATCTTTCTCTTCTCTTCTCTACTAAAATCAACATCACTTCCACTCTTCCCCTTTCTTCTTGAATTCTTCAATTCCTCACAAACCCAATTCAATCAAATAATTTCCCAATTTCCACAAAACCCTAACCCTATGGAACTTTCTCGTCTCTCACTTTCCGACAACTACTTCTTCTTATCCTCGCCAATTCATCGTTCTTCTTGGACTCGCCCGCTTCCCGCGTTGCCCGCATTCTTCACCACCCGCCGCCGCCGGAGAGGTTTATGCTCCGCCTCCAACTCCGACACTCTAGTCGCCGCTGCCGCCAAGAAGCACGGAGAGGATGACACTGACTTGAAGACGTGGATGCATAAGAATGGGCTTCCGCCTTGTAAGGTTGTTCTCAAGGATAAACCTTCGCTTGATGATAGTCTTAAGCCTATACACTATGTTGCTGCTAGCGAAGATCTTCAGAAGGGGGATGTTGCATTCTCGATTCCCAGTTCGTTGGTTGTCACTCTCGAGCGTGTATTGGGAAACGAGACTATCGCTGAGCTATTGACCACAAACAAGTTTTCGGAATTGGCATGCTTGGCCTTATATCTGATGTATGAGAAAAAACAGGGGAAGAAATCTTTCTGGTATCCATACATTAGGGAGCTTGATCGGCAACGAGGCAGGGGACAGCTGGCAGTGGAATCACCTCTTCTATGGTCAGAATCTGAGCTGGCATACCTTGAAGGAAGCCCATTAAAGGATGAAATTGTTAAAAGGATTGAAGGAATAAGAAAAGAGTATAATGAACTAGACTTAGTCTGGTTTATGTCTGGTTCTTTGTTTCAGCAATACCCATATGACATTCCCACCGAGGCTTTTCCATTTGAAATTTTCAAACAAGCTTTTGCAGCAGTTCAGTCATGTGTTGTGCATTTACAGAATGTTAGTTTAGCTCGGAGATTTGCTCTGGTTCCCCTGGGACCTCCTTTGTTAGCCTACTGTAGCAATTGCAGGGCGATGTTATCTGCGGTTGATGGTGCTGTCGAGCTTGTGGTTGATCGCCCATATAAAGCCGGGGACCCAATTGTTGTGTGGTGTGGCCCACAGCCTAACACAAAGTTACTTACAAACTATGGTTTTGTTGATGATGACAATTCCAATGATCGTCTCATAGTTGAGGCGGCTTTGAGTACCGAAGATCCACAATATCAAGAAAAAAGAATGGTTGCTCAAAGAAATGGAAAATTATCAATTCAAACTTTTAATGTGTATACTGGGAAGGAAAGGGAATCTGTCTTAGATATGATTCCGTATCTGCGTTTAGGCTATGTTTCAGATCCTTCTGAGATGCAATCTGTCATCTCCTCTGAAGGTCCAGTTTGTCCTATGAGCCCATGTATGGAGCGGGCCGTGCTGGACCAGCTTGCTGATTATTTCAATTCCCGGTTGGCTGCCTACCCTACAACCTTGGCTGAAGATGAATCTATGCTGGCAGATGGTAGTTTGAATCCAAAGAGGCGAGTTGCTACTCAACTTGTAAGGCTAGAAAAGAAAATGCTTCATGCATGTTTGCAGGCAATCACGGATTTCATAAACCAGCTACCAGACCATAGTGTATCGCCATGCCCTGCTCCTTATGCACCTTCATTAAAATAAAAGAATAACACCATGATTGCTGTACTTAGAACAATGATGCTATGCCTTTCCAAGTCCAGATTAAAAGGGATAGGTTCAAGTATTTTCTGCAGGCCGCATCAAGTTGGGGAGCATCATTGAAGCACTCAACTCCCTAGAAAATGTCGAACTGTGGTCGTGCAAATGATTCTAAATTTGAGTGTTGAAATGAAAGTAATCTTTGTAAATGGAAATATGTATCACAAATTGTAATTCATTTGTGTATATATTTGTATGCAGTTCTCGGGATTATATTTGATCTATTTTTTTTACTTTTGTTACTTTGTAGCCTACCTCTGTGTGGTCACTTNAANTTAGTTACTAAATANNTCAAATGTTATTAAAAGG
TARGET=285,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1924
PRIMER_LEFT_PENALTY=0.014945
PRIMER_RIGHT_PENALTY=0.177475
PRIMER_LEFT_SEQUENCE=ATTTCCACAAAACCCTAACCCT
PRIMER_RIGHT_SEQUENCE=AAGTCAGTGTCATCCTCTCCGT
PRIMER_LEFT=152,22
PRIMER_RIGHT=376,22
PRIMER_LEFT_TM=59.985
PRIMER_RIGHT_TM=60.177
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.1000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.2462
PRIMER_LEFT_1_PENALTY=0.014945
PRIMER_RIGHT_1_PENALTY=0.231272
PRIMER_LEFT_1_SEQUENCE=ATTTCCACAAAACCCTAACCCT
PRIMER_RIGHT_1_SEQUENCE=CGTCTTCAAGTCAGTGTCATCC
PRIMER_LEFT_1=152,22
PRIMER_RIGHT_1=383,22
PRIMER_LEFT_1_TM=59.985
PRIMER_RIGHT_1_TM=59.769
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.2642
PRIMER_LEFT_2_PENALTY=0.014945
PRIMER_RIGHT_2_PENALTY=0.249232
PRIMER_LEFT_2_SEQUENCE=ATTTCCACAAAACCCTAACCCT
PRIMER_RIGHT_2_SEQUENCE=CAAGCGAAGGTTTATCCTTGAG
PRIMER_LEFT_2=152,22
PRIMER_RIGHT_2=444,22
PRIMER_LEFT_2_TM=59.985
PRIMER_RIGHT_2_TM=60.249
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.2796
PRIMER_LEFT_3_PENALTY=0.102139
PRIMER_RIGHT_3_PENALTY=0.177475
PRIMER_LEFT_3_SEQUENCE=TCCACAAAACCCTAACCCTATG
PRIMER_RIGHT_3_SEQUENCE=AAGTCAGTGTCATCCTCTCCGT
PRIMER_LEFT_3=155,22
PRIMER_RIGHT_3=376,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.177
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.2796
PRIMER_LEFT_4_PENALTY=0.102139
PRIMER_RIGHT_4_PENALTY=0.177475
PRIMER_LEFT_4_SEQUENCE=CACAAAACCCTAACCCTATGGA
PRIMER_RIGHT_4_SEQUENCE=AAGTCAGTGTCATCCTCTCCGT
PRIMER_LEFT_4=157,22
PRIMER_RIGHT_4=376,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=60.177
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_05708 Ps_contig_mira-and-tgicl-ass_franssen_39577
SEQUENCE=GGTTGTCTTTGTTTGAATTTAAGATCAAATCTTCTAAGAGTTGGAAGATCTTCATAATGTAATCAAAGTCAAGTTGCTTGAAATCTTCACTATCAATAACATCTTCTACTTACTTGATAAGTGTCTTCAATACTCTAAATCACCAGTGTAATAATCTTTGGGGAGATAAATGTCTTTGAGAGGACCAAATTGACCAAAGGGTCTACGAAGATCTTCAGGCCTACAGTCATGGCGAAGGTTACGAACAAGAAGACTGGGTAGGTGGATCACTTTCACGGGGGGCGTAACGGCGACGGGGACTTGGACTGCGTCCTCCTCCTCCTCNCNCNCNCNCTCTCTCTCTCTCGTCGTCGTCGGTCGGTCGGTACGGTGGTAGGGTTAGGGTTACGGTTACGGTTCCGTTTCCGTTTACCGTTTACCGTTTCCGTTTACCGTTTACCGTTTTACCTTTTACCTTTTACCTTTTAC
TARGET=311,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1571
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.151478
PRIMER_LEFT_SEQUENCE=AGAAGACTGGGTAGGTGGATCA
PRIMER_RIGHT_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT=247,22
PRIMER_RIGHT=445,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.151
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.1750
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.151478
PRIMER_LEFT_1_SEQUENCE=GAGAGGACCAAATTGACCAAAG
PRIMER_RIGHT_1_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=445,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=0.1750
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.151478
PRIMER_LEFT_2_SEQUENCE=CTTTGAGAGGACCAAATTGACC
PRIMER_RIGHT_2_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_2=173,22
PRIMER_RIGHT_2=445,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.151
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.1826
PRIMER_LEFT_3_PENALTY=0.031122
PRIMER_RIGHT_3_PENALTY=0.151478
PRIMER_LEFT_3_SEQUENCE=ACAAGAAGACTGGGTAGGTGGA
PRIMER_RIGHT_3_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_3=244,22
PRIMER_RIGHT_3=445,22
PRIMER_LEFT_3_TM=60.031
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=202
PRIMER_PAIR_PENALTY_4=0.1846
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.151478
PRIMER_LEFT_4_SEQUENCE=GGTTACGAACAAGAAGACTGGG
PRIMER_RIGHT_4_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_4=236,22
PRIMER_RIGHT_4=445,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_05709 Ps_contig_mira-and-tgicl-ass_franssen_39577
SEQUENCE=GGTTGTCTTTGTTTGAATTTAAGATCAAATCTTCTAAGAGTTGGAAGATCTTCATAATGTAATCAAAGTCAAGTTGCTTGAAATCTTCACTATCAATAACATCTTCTACTTACTTGATAAGTGTCTTCAATACTCTAAATCACCAGTGTAATAATCTTTGGGGAGATAAATGTCTTTGAGAGGACCAAATTGACCAAAGGGTCTACGAAGATCTTCAGGCCTACAGTCATGGCGAAGGTTACGAACAAGAAGACTGGGTAGGTGGATCACTTTCACGGGGGGCGTAACGGCGACGGGGACTTGGACTGCGTCCTCCTCCTCCTCNCNCNCNCNCTCTCTCTCTCTCGTCGTCGTCGGTCGGTCGGTACGGTGGTAGGGTTAGGGTTACGGTTACGGTTCCGTTTCCGTTTACCGTTTACCGTTTCCGTTTACCGTTTACCGTTTTACCTTTTACCTTTTACCTTTTAC
TARGET=334,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1571
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.151478
PRIMER_LEFT_SEQUENCE=AGAAGACTGGGTAGGTGGATCA
PRIMER_RIGHT_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT=247,22
PRIMER_RIGHT=445,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.151
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.1750
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.151478
PRIMER_LEFT_1_SEQUENCE=GAGAGGACCAAATTGACCAAAG
PRIMER_RIGHT_1_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=445,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=0.1750
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.151478
PRIMER_LEFT_2_SEQUENCE=CTTTGAGAGGACCAAATTGACC
PRIMER_RIGHT_2_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_2=173,22
PRIMER_RIGHT_2=445,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.151
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.1826
PRIMER_LEFT_3_PENALTY=0.031122
PRIMER_RIGHT_3_PENALTY=0.151478
PRIMER_LEFT_3_SEQUENCE=ACAAGAAGACTGGGTAGGTGGA
PRIMER_RIGHT_3_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_3=244,22
PRIMER_RIGHT_3=445,22
PRIMER_LEFT_3_TM=60.031
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=202
PRIMER_PAIR_PENALTY_4=0.1846
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.151478
PRIMER_LEFT_4_SEQUENCE=GGTTACGAACAAGAAGACTGGG
PRIMER_RIGHT_4_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_4=236,22
PRIMER_RIGHT_4=445,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_05710 Ps_contig_mira-and-tgicl-ass_franssen_39577
SEQUENCE=GGTTGTCTTTGTTTGAATTTAAGATCAAATCTTCTAAGAGTTGGAAGATCTTCATAATGTAATCAAAGTCAAGTTGCTTGAAATCTTCACTATCAATAACATCTTCTACTTACTTGATAAGTGTCTTCAATACTCTAAATCACCAGTGTAATAATCTTTGGGGAGATAAATGTCTTTGAGAGGACCAAATTGACCAAAGGGTCTACGAAGATCTTCAGGCCTACAGTCATGGCGAAGGTTACGAACAAGAAGACTGGGTAGGTGGATCACTTTCACGGGGGGCGTAACGGCGACGGGGACTTGGACTGCGTCCTCCTCCTCCTCNCNCNCNCNCTCTCTCTCTCTCGTCGTCGTCGGTCGGTCGGTACGGTGGTAGGGTTAGGGTTACGGTTACGGTTCCGTTTCCGTTTACCGTTTACCGTTTCCGTTTACCGTTTACCGTTTTACCTTTTACCTTTTACCTTTTAC
TARGET=345,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1571
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.151478
PRIMER_LEFT_SEQUENCE=AGAAGACTGGGTAGGTGGATCA
PRIMER_RIGHT_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT=247,22
PRIMER_RIGHT=445,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.151
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.1750
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.151478
PRIMER_LEFT_1_SEQUENCE=GAGAGGACCAAATTGACCAAAG
PRIMER_RIGHT_1_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=445,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=0.1750
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.151478
PRIMER_LEFT_2_SEQUENCE=CTTTGAGAGGACCAAATTGACC
PRIMER_RIGHT_2_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_2=173,22
PRIMER_RIGHT_2=445,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.151
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.1826
PRIMER_LEFT_3_PENALTY=0.031122
PRIMER_RIGHT_3_PENALTY=0.151478
PRIMER_LEFT_3_SEQUENCE=ACAAGAAGACTGGGTAGGTGGA
PRIMER_RIGHT_3_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_3=244,22
PRIMER_RIGHT_3=445,22
PRIMER_LEFT_3_TM=60.031
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=202
PRIMER_PAIR_PENALTY_4=0.1846
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.151478
PRIMER_LEFT_4_SEQUENCE=GGTTACGAACAAGAAGACTGGG
PRIMER_RIGHT_4_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_4=236,22
PRIMER_RIGHT_4=445,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_05711 Ps_contig_mira-and-tgicl-ass_franssen_39577
SEQUENCE=GGTTGTCTTTGTTTGAATTTAAGATCAAATCTTCTAAGAGTTGGAAGATCTTCATAATGTAATCAAAGTCAAGTTGCTTGAAATCTTCACTATCAATAACATCTTCTACTTACTTGATAAGTGTCTTCAATACTCTAAATCACCAGTGTAATAATCTTTGGGGAGATAAATGTCTTTGAGAGGACCAAATTGACCAAAGGGTCTACGAAGATCTTCAGGCCTACAGTCATGGCGAAGGTTACGAACAAGAAGACTGGGTAGGTGGATCACTTTCACGGGGGGCGTAACGGCGACGGGGACTTGGACTGCGTCCTCCTCCTCCTCNCNCNCNCNCTCTCTCTCTCTCGTCGTCGTCGGTCGGTCGGTACGGTGGTAGGGTTAGGGTTACGGTTACGGTTCCGTTTCCGTTTACCGTTTACCGTTTCCGTTTACCGTTTACCGTTTTACCTTTTACCTTTTACCTTTTAC
TARGET=353,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1571
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.151478
PRIMER_LEFT_SEQUENCE=AGAAGACTGGGTAGGTGGATCA
PRIMER_RIGHT_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT=247,22
PRIMER_RIGHT=445,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.151
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.1750
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.151478
PRIMER_LEFT_1_SEQUENCE=GAGAGGACCAAATTGACCAAAG
PRIMER_RIGHT_1_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=445,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.151
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=0.1750
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.151478
PRIMER_LEFT_2_SEQUENCE=CTTTGAGAGGACCAAATTGACC
PRIMER_RIGHT_2_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_2=173,22
PRIMER_RIGHT_2=445,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.151
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.1826
PRIMER_LEFT_3_PENALTY=0.031122
PRIMER_RIGHT_3_PENALTY=0.151478
PRIMER_LEFT_3_SEQUENCE=ACAAGAAGACTGGGTAGGTGGA
PRIMER_RIGHT_3_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_3=244,22
PRIMER_RIGHT_3=445,22
PRIMER_LEFT_3_TM=60.031
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=202
PRIMER_PAIR_PENALTY_4=0.1846
PRIMER_LEFT_4_PENALTY=0.033137
PRIMER_RIGHT_4_PENALTY=0.151478
PRIMER_LEFT_4_SEQUENCE=GGTTACGAACAAGAAGACTGGG
PRIMER_RIGHT_4_SEQUENCE=TAAAACGGTAAACGGTAAACGG
PRIMER_LEFT_4=236,22
PRIMER_RIGHT_4=445,22
PRIMER_LEFT_4_TM=60.033
PRIMER_RIGHT_4_TM=60.151
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=210
=
PRIMER_SEQUENCE_ID=SSR_05712 Ps_contig_mira-and-tgicl-ass_franssen_39583
SEQUENCE=TTTATAGTTCCATGTGCCATCTGCCAATGACATACCATTATGGTCTGTTGCATATTCTTCAAGCATGAAGAACCCCAAACCCTGGACAAAAGCTCCTTCAATCTGTCCTAAATCCACAGCAGGATTGAGACTTTGTCCACAATCATAGATGATATCTGTTTGAAGAAATTTGGTTCCTCCAGTCAGAAGATCTATTTCCACCTCACTTACTGCAGCACCATAATTAAGGTACATTGTCGAGTTATTGCTGGGTACGTATAATGAAGACGCTGATAAGTTCACAGATTGCATGTATGCCTGAAGAATAAGTGCCTCCCACTTGATAGAGCCCATTTCTTCCTGCAGCTTTTCCTTGAGAGGCTTCAACCTCTCAACCAAGGTATCACAGCTAAGCCTAACTGCTTCACAGCATGTCTCTGATGTGGTGCTCCCAGCAGTAAACCCCCCTTGAATCAAGCTCACCGTGTCAGCTTGTACAACCCTTACTTTGTCTAAGAGACTTTCAGTTTCATCACATTGAATTGTACCGAGGGCAAATGCAGCCATCTGTTTCACCTTTGTCCATAGACCCTGACCCAGTTCAATTCCTCCAACTTCAACAACAACAGACCCATCCGACAAAATACTCACTTTTCCAGGAGTTGGTCTAAGAGACAGTTGATACACAACTGGTACTCGAGAAATTCCCCTTTTTCTCCAAATATTAATTCTGTTAAACTCTTTCACCATTTCGGTTCTTTGTTCATAATTTGCAGAAACAGCTATCTTACTCCATATTGAAGGCAATGTATACTCAAAAGGTTCGCCATAACAATGATCATAGAATGATTGAAGACTTTTGTGCGTGTGAAGATTAATGCTTCTGACAGAATCTACATCTATTGAAAGTGTAGCTGCAACATTTTCTATAATATGTTCAGCGATAAATGATCCCTGCACTTCCCCAGGGCCCCTCATTGCAGATCTACTGGGAAGATTTGTTCGGCATACCTTTATATCAAAAGATAGTGCACCCCAGTCATACTTTTTTAGTGCTGTAACTATGTTATGTGGCATTACTACACTTATATCCATATATATACCAGCATCGACTAATATCTCAAGTTCTAATGCAGTTATTTTCCCATCATTCTTAAACCCAGCACTGTATGTAATCTTCATGGGATGTCTTCCTCCAGCCAATATCATATCAGTCTTTCGATTCAGATACATCCTGACAGGGCGACATAATTTATGTGCTGCGAGTGCACAAGCTGCAGCAGTAGATATTGCTTTTATGGCCTTTCCTCCAAAACCTCCCCCGACCCTTCTTGTAATTACTCGTACATTATTTTCAGGAATACCAAGGCATCTTGCTATAGTAGAATGAGTAAATTCAGGGCACTGATTTGAAGCGTAAACTGTAATGCAATTGTCTTCGTCNGGCACAGCAAGTGCAGTTTGTGTCTCCATATAGAAATAGTATTGAGACCCTAGYYYNAACTCGGCAGAAAGAATTTTGTGATCAGCTTCAGCCATTCCTTTTGATATATCACCAACATTTTTTGGTAAAGAGAAAAGAAGGAACTTCAAAAAAGCTTGATCTTTTAACTGCATCTTCAACAGATAGAATAGGTGATTCAAGGTTTTCAATATCATAACTAACAATGGTTGAGTTTGCTGCCATATCTGCAAGTTTCTGACTATCTGCAACCACAACAGCCAGACGATCGCCAACACATCTGGCTATCTCTTCTACAAATAAAGGCTCTGTGCCAAATATTGTCTTAGATCCAATGTTTTCTCCTCCAATGGGAATGTCTTTACTTGAAATGACATCTCTTACTCCATCCAGCTGCAATTTAGGGCTGAGTTTTATATTCTTTACCCTTGCCAAAGGTTTTGAACTATATATATAAGCTCCATGTAGGCAATTTGGTGGTGAAGGAATGTCATCCACAAACACAGCCTCGCCTGAAGCTTGTAGGGCAGCTCCTGACTTAGTGATTGTTTTTCCAATGGGGTGATATTCATTTCCTGCTTCAAGGATTTGTTTCCCAGAAGACAATAAAGTTGGAGTGTTGTCATGATGAACCTGCTTCTGATTCTCTTTTAGTTCAAAATCCTTCACAAAAGGAAAACTAGAATATCCATTTGAGAAGTTGGTCGCTCCCGCAGAAATTTCAATTAGAGGGTTAAAGAACTGAAAAACAAAACTAGCTGCCAAACTTGAACGGTATGCAGTTTTTGAGTTTTCATCTTTAGGGACAATAGAGGATGTAAGCAAGTTGACGGCTTCATACAGAATGCTAAAACTTAACATCTTTCCTGTTAAAAATTCCTCAACATTTTTTGCTCTGATTGTGTGCTCATTCCCATTAGCACCAAAAGACAGCCTACAAGTGTCTATCATGGAACCACCAGAATCTTCGCTAGGAGACACCTCAACAAGGAAAGCGGCATTTAAGTATGGAAGGGCATTTCCAAGCGGTCTAGGAGAAGCTCGGTAAGTTTCAAAGTAGAATCTATTTCTTTGTTTTGAAGATGTACTTCTAATGGTTTCCAAATTTGGAATTTTAATGCTTAAAAGTACACTTTCCAAACTTAATGGCGGTCTTTCCAAAAATTCCTCCAACGTTAACCATTCAAACTGTGTGCCACTCATTATATGAACCATCGAATCCACAGCAAGAAGTATAGTAGCGATATCTGAAGGAAAATTGTTCTTTTGTGCCATAATTAAATTTCCACCTAGACTGGCCGTGTTTCTAATAAACCCCGAAGCAACTTTTCCAAAATGGTCAGCAATCTTTTCAAGTATCATTACAAAGTCTGAGATAAAGTCACTCTTGCTTTGCTGCTTTAGTACTTCAATAGCGTTAGATATGGTCACTGCTGCTCCAATTTCAATCCCTGATTGATCCTTTCTGATCTTTGAGAGCTCAGAAATGCCCTTTAGATCAATATACTTATCATATCCTTCTTTATCTTTGTAATATCCCATACCGGTATTATTAGCAACAACTTTTATCCTGGTTCCGTTGGCATGGTTTAATTCCAATAAGTTCTGAAGCTCCTCTAAACTAGTAGGTTTGTGCCAACTGTTTTTCTTAGAAGCCAACAACAAATCATGTTTAATATCCTTCAGAAACATAGGAAATTTGATGTTCTTGTGATGATTGTCATATTGAGGCAACCTACTAAGCTTCAAGTCCTTGCTCTCTCCCTTTCTCCAGAAAGAGTTCAACCCTAAATCCTCCATATCAACATCAGCTGCGAAACTTTTGCAGGCATCAGCAATTGGCCGGTAGCCAGTGCAGCGGCAAAGGTTCCCTGCAATAGCCTTTTCAGCCTCAAAACCAGTAACTTTGGAGAATCCAGAAGGTGGCTCTGGACGATTATTCTTTTCAGCATTGACAAGAGCTCCAAAGAGGGAAACGCACATTCCAGGAGTACAAAAGCCACATTGAGAAGCATGGAATCCAGCAAATCTTTCATGAATCGGATGAAATCCTTGTTTGCTATTTCCAATGCCTTCACTTGTTGTTATTGAACATCCATGTATGCTACAAAGTAGTGTGAGACAAGAGCTTGCAGTGAAATCTTCAATTCTGTCAAGCAATGGATCATATTTGGATATTATAACTACGCAAGCACCACAACCACCTTCGCCACAACCGAGTTTAACACTCTTGAATCGAGTTTGAGTACGCAAGAACTCAAGCAAAGTGGTGGACGAGTCGACGGTGGATAGCTCAAACTTTTCGCCATTGATAACGAAAACCAAACTTGTTTGATTCTCACTCATCTTCACTTCCATTCTTCAATAACTGATTTTCTGAAAAATACAAGTTACAATGTATCTGCATACTATTTATATTCGCTTATAGCGTAGCAATCGCTTCACTATAGAATAAGAAGAAAAAAGTGATTTTAATTAAAGTCTTGAAT
TARGET=1888,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05713 Ps_contig_mira-and-tgicl-ass_franssen_39595
SEQUENCE=TTAATTAATTAACAATTCTAGTTGTCTAGCACTTCCTTTCCCTAACCAAAATTTACTCTCAAGCTACAAAGAGCCTCTGCAGAGAGGCTGCAGCATCTCAGTTTCCGTTGGGGAGGGTGGGGATGGCTCTGACTCCCCATGGTCAATGGAAGACAGACATATGATGATCACTGTAAGATTATCGAATGTATTGAGGCGTAATGCCTCCATAACTAGATCCCTGGCGCACTTATCTGGGTCATCATGCCTCCTCAAGCCTTTACGTACCAGGCTAACGGCATGCTGGCTTGACATCACATCCCAGATGCCATCACATCCTATGATGAGGAATTCGTCGTCATCTGTTAGAACCATTTGACGGAACTCGGGCTCTGCAATCAAAGGCGAGGGAACACCCCTAGGTAACTTCATGTCCCAGTCCCCTAATGCTCTGGTAACTGATAAAACGCCATTTAGGTAACCATCTTCTACATAGCCGCCCAATTCTTCAACTCGTCTCCGCTCTATTGGATAAATAGGCCGGTGATCTTGAGACATATCAATGGCCTCTCCTTTACGACTGAGAACTGCTCGACAGTCACCAGCATTGGCCACCATTAGAAGTCTTCCAAATATCATAGCAGTAAGAGCTGTGGTCCCTGAAGAAGTGTTCACACTGCAATCATCAGCCATAGCCGAATCCGCCAAAAGAAAAGCCTTTCTGAGGGAATTCTCAACCTCTTGTAAAAATATGTTATCAACTTCAGATGTCTGAGGGAAATTGACATCCTCAAAGAAGAATTTTATAACATTTTTCTTATGTAAGCTGCTGCTTCAGGTCCTCCATGACCATCAAACACCCCGTAAAAGGCACTTGG
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05714 Ps_contig_mira-and-tgicl-ass_franssen_39598
SEQUENCE=GAACTGCTTACACTTTCTGTGTTCCGGCAATGCCGCAAGCCTTTCCATTTCAAGGAAATCGTCCATCAGATCATTCTGAGTTGAAAGGCCTATATGATTTTGTGCCACGGCATTCAGTCTCTGCCATTCGGTGTCAAGCTTAGCCACCTTTTTTAAACTCTCCAAATGTTGCTTGCTTGCTGCTTCGGCAGATTCGGTACTCAAATCCCTTTCAATGGTTTTGAATTCAAGTTCCTCGAGCCGAGATTGCAGTTCAATTTTAAGACACGAATTCTCCCTCTCCACATCCTCAAGCCTCTGTAGAAGACCAAGAATAGAACTGA
TARGET=169,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6351
PRIMER_LEFT_PENALTY=0.159665
PRIMER_RIGHT_PENALTY=0.475463
PRIMER_LEFT_SEQUENCE=GGAAATCGTCCATCAGATCATT
PRIMER_RIGHT_SEQUENCE=GGTCTTCTACAGAGGCTTGAGG
PRIMER_LEFT=53,22
PRIMER_RIGHT=308,22
PRIMER_LEFT_TM=60.160
PRIMER_RIGHT_TM=59.525
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=256
PRIMER_PAIR_PENALTY_1=0.6501
PRIMER_LEFT_1_PENALTY=0.174657
PRIMER_RIGHT_1_PENALTY=0.475463
PRIMER_LEFT_1_SEQUENCE=GTGTCAAGCTTAGCCACCTTTT
PRIMER_RIGHT_1_SEQUENCE=GGTCTTCTACAGAGGCTTGAGG
PRIMER_LEFT_1=130,22
PRIMER_RIGHT_1=308,22
PRIMER_LEFT_1_TM=59.825
PRIMER_RIGHT_1_TM=59.525
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=179
PRIMER_PAIR_PENALTY_2=0.6634
PRIMER_LEFT_2_PENALTY=0.159665
PRIMER_RIGHT_2_PENALTY=0.503696
PRIMER_LEFT_2_SEQUENCE=GGAAATCGTCCATCAGATCATT
PRIMER_RIGHT_2_SEQUENCE=TCTACAGAGGCTTGAGGATGTG
PRIMER_LEFT_2=53,22
PRIMER_RIGHT_2=303,22
PRIMER_LEFT_2_TM=60.160
PRIMER_RIGHT_2_TM=59.496
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.6784
PRIMER_LEFT_3_PENALTY=0.174657
PRIMER_RIGHT_3_PENALTY=0.503696
PRIMER_LEFT_3_SEQUENCE=GTGTCAAGCTTAGCCACCTTTT
PRIMER_RIGHT_3_SEQUENCE=TCTACAGAGGCTTGAGGATGTG
PRIMER_LEFT_3=130,22
PRIMER_RIGHT_3=303,22
PRIMER_LEFT_3_TM=59.825
PRIMER_RIGHT_3_TM=59.496
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.6808
PRIMER_LEFT_4_PENALTY=0.205314
PRIMER_RIGHT_4_PENALTY=0.475463
PRIMER_LEFT_4_SEQUENCE=AGGAAATCGTCCATCAGATCAT
PRIMER_RIGHT_4_SEQUENCE=GGTCTTCTACAGAGGCTTGAGG
PRIMER_LEFT_4=52,22
PRIMER_RIGHT_4=308,22
PRIMER_LEFT_4_TM=59.795
PRIMER_RIGHT_4_TM=59.525
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_05715 Ps_contig_mira-and-tgicl-ass_franssen_39600
SEQUENCE=TGTGTCTAGGTTTTGAAGTTTCCCCATCTCCGGCGGTAACTCGCCGGATAAACCGCAGTAAGCTGCGTCAAGACGGATAAGCTCAGTCAAGTTTCCAATCTCCGGAGGAATGCCACCGGTGTACGTGTTGAAATATCCGATGTAAAGCTCACGGAGACTGGTTAAGTTTCCGATCTCCGGCGGGATAGTTCCGGCAAGTTCGTTACCGGAAACAGCTAAATACTCCAAGTGTTGCCATAGTCCAAACTCCGGCGGGATCTGACCGGTAAAGTAGTTACCACCGAGATGCAAATGACGGAGATTCGGCAACTCGGTAACAGCAAGAGGAAGTGTTCCTGTCATGTTGTTGTTGTA
TARGET=342,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05716 Ps_contig_mira-and-tgicl-ass_franssen_39608
SEQUENCE=CCACATCTTGACCTGCTTATCACAACCTCCRGAAAAAACCGTTGTTCCATCATCCTTCCAAGCATGGCACAAAACCGGTTGATCATGAGAAATGGAAGCCTTAGGAGTACTGTTAAGAGCAGTTCCATTCTTCGAAATCTCCCAACACCGAACCTGGTTGTCCCATGAAGTAGCAACGAGGAAATTGGCCTTGGAACTGAAAGAAATACTGGAAACAGAATCAGAAGGTGGTTGCGTAACCTCGAACGATTTGTTAGGATTTGTATTTGCACCTCCGAAACTCGACATTTTGATCAACTTCGAGAGAGAGAGTTTCTGGGTGGTTCTTGCGTCCGACTAA
TARGET=302,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05717 Ps_contig_mira-and-tgicl-ass_franssen_39612
SEQUENCE=GCTTCTGCAGCCTTCCTCCATTGATCAGACTGTACCTTAAGCTTCCGCAGTTCGCTTTCCATTTCCGAGCTTGCCGCTTGCGCTGCTTCTAACTGCTCGGTCACTCTCGCGGCCTTTCGGTTGCTTCTGTCTGCTTCCTCCATTGCAATCGCGAGTTTCGTTGCCACCTCGCGTTCTGCAGCCTTGGCTTTCTCTATTTCGTCTCTCGCGTTTTCGACATCCGAACTGCGCTTGCTGATCTCCATCTTCAGCGTCTCGTTCTCTTCTGATATGGTTTGCAATGTTGTTTCCTTGTCCATCATATCGGCTTTCATTTCATCCAAACATTGTTCCAATCTTTTAACTTCCTTTGCAAGTCCATTATCATTTTGTGATGACATGTTGTTTTCGAGCTTCGAATTCAAGTTCTCGTTCTCTTCCATAATACCTTGCAACTCGGTTTCTTTGTCCATTAGATTAGCCTTCAATTCTTCAATATCGGCTTTCTTTCTTTGTAATTCAGCCTCCAACTCGCGCTGTCTGTCGCCTGACTCGGACTTTATCTGCTCCATCAACTCGTACGCGGATTTTATCTGCACCGTGGTTTGAATCTGTTCTTGTTGATATTTAGTCTCGGAAGTCTCTAGAGCAGATCTCAACCCTTCAACCTCGGACTTTAAAGAACAAATCTCAACTTCAATTTGATTAGGATCCTCTCCATCTTTCACTATTTCACATTCCTTCTCGAATTCGCTATCTTCTAGAAGATTTTGAATAGAATGAGTTGATTCATTATCATTAACGTGTGTCTTAAGTTTGCTAACAAGTGTCTCYAGCGTGTTCACCCTTACTCGAGAATGTTCTAATTCCAAAGCAATGGAGTTGTAGTCATCTACATCTTTCACAACATCGTCTGCTAGAATCTCAACGGTTCTTTTCGCAGCTTCGAGTTGGAACAAAGCTTCATCGACA
TARGET=482,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05718 Ps_contig_mira-and-tgicl-ass_franssen_39618
SEQUENCE=ATCACTCTGGTGGACTCAACACCATCAACAACAACAACCTATGATGTTACTAGAACCAATCCAAACCACTCCTTGGCCACTCCACCACCACCAACCACCATGGTTCACTTCACAAATCCATGAAAACGAAGATGAACAAGTAGAAAACGACACCACCGTTGTAATAAAACCCGAAGACGAAAAAGAACCAATGTTCGAGAAACCATTAACACCAAGCGACGTAGGAAAGCTCAACCGTCTGGTTATCCCAAAGCAATACGCGGAAAGATACTTCCCACTCGGCGGTGGTGACTCGGGTGAGTGCAAGGGCTTGTTACTGAGTTTCGAGGACGAGTCAGGGAAGTGTTGGCGATTCCGTTACTCGTATTGGAACAGTAGTCAAAGCTACGTGTTGACTAAAGGTTGGAGTCGTTATGTGAAAGATAAGCGTCTCGATGCTGGCGACGTCGTTTTGTTTGAACGACACCGTGTTGATAGTCAGCGTCTTTTTATCAACTGGAGGAGGAGGAATGGGAATGATGAGTCAGCGTATGTTAGCAGAGGGGTGGGACAACAAGGGAAGGGGGGTGATAAGAATGAAGTTGTAGGCGTGGGTGTTGGGTGGTCCAGAGGATTCTATTCTACGCACTTGCCTTATCCTGCGCATCACCATCATCATCATCAGGTTTTGTCATACCAACATGAGCACTGTCTTCATGGAGGTAGAGTGTCCCAAGAACAGAACCAAAGGACGAAACCGGTAGGAAACAGTTCAAGTTCAAGGGTACTTAGGTTGTTTGGTGTTAACATGGAATGCCAACCAGAACATGAACATGAACATGATTCTTCTTCAACACCTCAATGTTCTTATGATACTAACAACAATAATAATATGTCACTAACACAATATCATCAACCTAATACTTCTAATTCTAACCCTCACGTGGTACGTCACCAACCATATTATTACTAGATATCTATCTATGTATACAACCTTTGAGGTTGTATTTTTAGTTATTTTTGTCTTTTTTTTAATTAGGTGTAAGATGTATTTAACTATGTATCGACTTTGGAAATTATAATTATTTCTAATTAATTAGTTTATGACTT
TARGET=26,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8737
PRIMER_LEFT_PENALTY=0.863624
PRIMER_RIGHT_PENALTY=0.010077
PRIMER_LEFT_SEQUENCE=ACTCTGGTGGACTCAACACCAT
PRIMER_RIGHT_SEQUENCE=GGAAGTATCTTTCCGCGTATTG
PRIMER_LEFT=3,22
PRIMER_RIGHT=274,22
PRIMER_LEFT_TM=60.864
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.8994
PRIMER_LEFT_1_PENALTY=0.863624
PRIMER_RIGHT_1_PENALTY=0.035772
PRIMER_LEFT_1_SEQUENCE=ACTCTGGTGGACTCAACACCAT
PRIMER_RIGHT_1_SEQUENCE=ACGGTGGTGTCGTTTTCTACTT
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=158,22
PRIMER_LEFT_1_TM=60.864
PRIMER_RIGHT_1_TM=59.964
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.8000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.8994
PRIMER_LEFT_2_PENALTY=0.863624
PRIMER_RIGHT_2_PENALTY=0.035772
PRIMER_LEFT_2_SEQUENCE=ACTCTGGTGGACTCAACACCAT
PRIMER_RIGHT_2_SEQUENCE=AACGGTGGTGTCGTTTTCTACT
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=159,22
PRIMER_LEFT_2_TM=60.864
PRIMER_RIGHT_2_TM=59.964
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=157
PRIMER_PAIR_PENALTY_3=0.9569
PRIMER_LEFT_3_PENALTY=0.921150
PRIMER_RIGHT_3_PENALTY=0.035772
PRIMER_LEFT_3_SEQUENCE=ATCACTCTGGTGGACTCAACAC
PRIMER_RIGHT_3_SEQUENCE=ACGGTGGTGTCGTTTTCTACTT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=158,22
PRIMER_LEFT_3_TM=59.079
PRIMER_RIGHT_3_TM=59.964
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=0.9569
PRIMER_LEFT_4_PENALTY=0.921150
PRIMER_RIGHT_4_PENALTY=0.035772
PRIMER_LEFT_4_SEQUENCE=ATCACTCTGGTGGACTCAACAC
PRIMER_RIGHT_4_SEQUENCE=AACGGTGGTGTCGTTTTCTACT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=159,22
PRIMER_LEFT_4_TM=59.079
PRIMER_RIGHT_4_TM=59.964
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_05719 Ps_contig_mira-and-tgicl-ass_franssen_39618
SEQUENCE=ATCACTCTGGTGGACTCAACACCATCAACAACAACAACCTATGATGTTACTAGAACCAATCCAAACCACTCCTTGGCCACTCCACCACCACCAACCACCATGGTTCACTTCACAAATCCATGAAAACGAAGATGAACAAGTAGAAAACGACACCACCGTTGTAATAAAACCCGAAGACGAAAAAGAACCAATGTTCGAGAAACCATTAACACCAAGCGACGTAGGAAAGCTCAACCGTCTGGTTATCCCAAAGCAATACGCGGAAAGATACTTCCCACTCGGCGGTGGTGACTCGGGTGAGTGCAAGGGCTTGTTACTGAGTTTCGAGGACGAGTCAGGGAAGTGTTGGCGATTCCGTTACTCGTATTGGAACAGTAGTCAAAGCTACGTGTTGACTAAAGGTTGGAGTCGTTATGTGAAAGATAAGCGTCTCGATGCTGGCGACGTCGTTTTGTTTGAACGACACCGTGTTGATAGTCAGCGTCTTTTTATCAACTGGAGGAGGAGGAATGGGAATGATGAGTCAGCGTATGTTAGCAGAGGGGTGGGACAACAAGGGAAGGGGGGTGATAAGAATGAAGTTGTAGGCGTGGGTGTTGGGTGGTCCAGAGGATTCTATTCTACGCACTTGCCTTATCCTGCGCATCACCATCATCATCATCAGGTTTTGTCATACCAACATGAGCACTGTCTTCATGGAGGTAGAGTGTCCCAAGAACAGAACCAAAGGACGAAACCGGTAGGAAACAGTTCAAGTTCAAGGGTACTTAGGTTGTTTGGTGTTAACATGGAATGCCAACCAGAACATGAACATGAACATGATTCTTCTTCAACACCTCAATGTTCTTATGATACTAACAACAATAATAATATGTCACTAACACAATATCATCAACCTAATACTTCTAATTCTAACCCTCACGTGGTACGTCACCAACCATATTATTACTAGATATCTATCTATGTATACAACCTTTGAGGTTGTATTTTTAGTTATTTTTGTCTTTTTTTTAATTAGGTGTAAGATGTATTTAACTATGTATCGACTTTGGAAATTATAATTATTTCTAATTAATTAGTTTATGACTT
TARGET=82,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2311
PRIMER_LEFT_PENALTY=0.220992
PRIMER_RIGHT_PENALTY=0.010077
PRIMER_LEFT_SEQUENCE=TAGAACCAATCCAAACCACTCC
PRIMER_RIGHT_SEQUENCE=GGAAGTATCTTTCCGCGTATTG
PRIMER_LEFT=50,22
PRIMER_RIGHT=274,22
PRIMER_LEFT_TM=60.221
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.2568
PRIMER_LEFT_1_PENALTY=0.220992
PRIMER_RIGHT_1_PENALTY=0.035772
PRIMER_LEFT_1_SEQUENCE=TAGAACCAATCCAAACCACTCC
PRIMER_RIGHT_1_SEQUENCE=ACGGTGGTGTCGTTTTCTACTT
PRIMER_LEFT_1=50,22
PRIMER_RIGHT_1=158,22
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=59.964
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=0.3073
PRIMER_LEFT_2_PENALTY=0.220992
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=TAGAACCAATCCAAACCACTCC
PRIMER_RIGHT_2_SEQUENCE=TCGTCCTCGAAACTCAGTAACA
PRIMER_LEFT_2=50,22
PRIMER_RIGHT_2=332,22
PRIMER_LEFT_2_TM=60.221
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=0.3427
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.121675
PRIMER_LEFT_3_SEQUENCE=TAGAACCAATCCAAACCACTCC
PRIMER_RIGHT_3_SEQUENCE=GAAGTATCTTTCCGCGTATTGC
PRIMER_LEFT_3=50,22
PRIMER_RIGHT_3=273,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=60.122
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=224
PRIMER_PAIR_PENALTY_4=0.3481
PRIMER_LEFT_4_PENALTY=0.338015
PRIMER_RIGHT_4_PENALTY=0.010077
PRIMER_LEFT_4_SEQUENCE=AACACCATCAACAACAACAACC
PRIMER_RIGHT_4_SEQUENCE=GGAAGTATCTTTCCGCGTATTG
PRIMER_LEFT_4=17,22
PRIMER_RIGHT_4=274,22
PRIMER_LEFT_4_TM=59.662
PRIMER_RIGHT_4_TM=59.990
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_05720 Ps_contig_mira-and-tgicl-ass_franssen_39618
SEQUENCE=ATCACTCTGGTGGACTCAACACCATCAACAACAACAACCTATGATGTTACTAGAACCAATCCAAACCACTCCTTGGCCACTCCACCACCACCAACCACCATGGTTCACTTCACAAATCCATGAAAACGAAGATGAACAAGTAGAAAACGACACCACCGTTGTAATAAAACCCGAAGACGAAAAAGAACCAATGTTCGAGAAACCATTAACACCAAGCGACGTAGGAAAGCTCAACCGTCTGGTTATCCCAAAGCAATACGCGGAAAGATACTTCCCACTCGGCGGTGGTGACTCGGGTGAGTGCAAGGGCTTGTTACTGAGTTTCGAGGACGAGTCAGGGAAGTGTTGGCGATTCCGTTACTCGTATTGGAACAGTAGTCAAAGCTACGTGTTGACTAAAGGTTGGAGTCGTTATGTGAAAGATAAGCGTCTCGATGCTGGCGACGTCGTTTTGTTTGAACGACACCGTGTTGATAGTCAGCGTCTTTTTATCAACTGGAGGAGGAGGAATGGGAATGATGAGTCAGCGTATGTTAGCAGAGGGGTGGGACAACAAGGGAAGGGGGGTGATAAGAATGAAGTTGTAGGCGTGGGTGTTGGGTGGTCCAGAGGATTCTATTCTACGCACTTGCCTTATCCTGCGCATCACCATCATCATCATCAGGTTTTGTCATACCAACATGAGCACTGTCTTCATGGAGGTAGAGTGTCCCAAGAACAGAACCAAAGGACGAAACCGGTAGGAAACAGTTCAAGTTCAAGGGTACTTAGGTTGTTTGGTGTTAACATGGAATGCCAACCAGAACATGAACATGAACATGATTCTTCTTCAACACCTCAATGTTCTTATGATACTAACAACAATAATAATATGTCACTAACACAATATCATCAACCTAATACTTCTAATTCTAACCCTCACGTGGTACGTCACCAACCATATTATTACTAGATATCTATCTATGTATACAACCTTTGAGGTTGTATTTTTAGTTATTTTTGTCTTTTTTTTAATTAGGTGTAAGATGTATTTAACTATGTATCGACTTTGGAAATTATAATTATTTCTAATTAATTAGTTTATGACTT
TARGET=498,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1014
PRIMER_LEFT_PENALTY=0.095831
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=ACGACACCGTGTTGATAGTCAG
PRIMER_RIGHT_SEQUENCE=TCTTGGGACACTCTACCTCCAT
PRIMER_LEFT=459,22
PRIMER_RIGHT=716,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=258
PRIMER_PAIR_PENALTY_1=0.1147
PRIMER_LEFT_1_PENALTY=0.095831
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=ACGACACCGTGTTGATAGTCAG
PRIMER_RIGHT_1_SEQUENCE=GTTTCGTCCTTTGGTTCTGTTC
PRIMER_LEFT_1=459,22
PRIMER_RIGHT_1=736,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.1890
PRIMER_LEFT_2_PENALTY=0.095831
PRIMER_RIGHT_2_PENALTY=0.093130
PRIMER_LEFT_2_SEQUENCE=ACGACACCGTGTTGATAGTCAG
PRIMER_RIGHT_2_SEQUENCE=TTGAACTGTTTCCTACCGGTTT
PRIMER_LEFT_2=459,22
PRIMER_RIGHT_2=754,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=59.907
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=296
PRIMER_PAIR_PENALTY_3=0.2398
PRIMER_LEFT_3_PENALTY=0.095831
PRIMER_RIGHT_3_PENALTY=0.143968
PRIMER_LEFT_3_SEQUENCE=ACGACACCGTGTTGATAGTCAG
PRIMER_RIGHT_3_SEQUENCE=TTCGTCCTTTGGTTCTGTTCTT
PRIMER_LEFT_3=459,22
PRIMER_RIGHT_3=734,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=60.144
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.2398
PRIMER_LEFT_4_PENALTY=0.095831
PRIMER_RIGHT_4_PENALTY=0.143968
PRIMER_LEFT_4_SEQUENCE=ACGACACCGTGTTGATAGTCAG
PRIMER_RIGHT_4_SEQUENCE=TTTCGTCCTTTGGTTCTGTTCT
PRIMER_LEFT_4=459,22
PRIMER_RIGHT_4=735,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=60.144
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=277
=
PRIMER_SEQUENCE_ID=SSR_05721 Ps_contig_mira-and-tgicl-ass_franssen_39618
SEQUENCE=ATCACTCTGGTGGACTCAACACCATCAACAACAACAACCTATGATGTTACTAGAACCAATCCAAACCACTCCTTGGCCACTCCACCACCACCAACCACCATGGTTCACTTCACAAATCCATGAAAACGAAGATGAACAAGTAGAAAACGACACCACCGTTGTAATAAAACCCGAAGACGAAAAAGAACCAATGTTCGAGAAACCATTAACACCAAGCGACGTAGGAAAGCTCAACCGTCTGGTTATCCCAAAGCAATACGCGGAAAGATACTTCCCACTCGGCGGTGGTGACTCGGGTGAGTGCAAGGGCTTGTTACTGAGTTTCGAGGACGAGTCAGGGAAGTGTTGGCGATTCCGTTACTCGTATTGGAACAGTAGTCAAAGCTACGTGTTGACTAAAGGTTGGAGTCGTTATGTGAAAGATAAGCGTCTCGATGCTGGCGACGTCGTTTTGTTTGAACGACACCGTGTTGATAGTCAGCGTCTTTTTATCAACTGGAGGAGGAGGAATGGGAATGATGAGTCAGCGTATGTTAGCAGAGGGGTGGGACAACAAGGGAAGGGGGGTGATAAGAATGAAGTTGTAGGCGTGGGTGTTGGGTGGTCCAGAGGATTCTATTCTACGCACTTGCCTTATCCTGCGCATCACCATCATCATCATCAGGTTTTGTCATACCAACATGAGCACTGTCTTCATGGAGGTAGAGTGTCCCAAGAACAGAACCAAAGGACGAAACCGGTAGGAAACAGTTCAAGTTCAAGGGTACTTAGGTTGTTTGGTGTTAACATGGAATGCCAACCAGAACATGAACATGAACATGATTCTTCTTCAACACCTCAATGTTCTTATGATACTAACAACAATAATAATATGTCACTAACACAATATCATCAACCTAATACTTCTAATTCTAACCCTCACGTGGTACGTCACCAACCATATTATTACTAGATATCTATCTATGTATACAACCTTTGAGGTTGTATTTTTAGTTATTTTTGTCTTTTTTTTAATTAGGTGTAAGATGTATTTAACTATGTATCGACTTTGGAAATTATAATTATTTCTAATTAATTAGTTTATGACTT
TARGET=650,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0797
PRIMER_LEFT_PENALTY=0.067516
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=TTTATCAACTGGAGGAGGAGGA
PRIMER_RIGHT_SEQUENCE=CCCTTGAACTTGAACTGTTTCC
PRIMER_LEFT=487,22
PRIMER_RIGHT=763,22
PRIMER_LEFT_TM=60.068
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=277
PRIMER_PAIR_PENALTY_1=0.0864
PRIMER_LEFT_1_PENALTY=0.067516
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=TTTATCAACTGGAGGAGGAGGA
PRIMER_RIGHT_1_SEQUENCE=GTTTCGTCCTTTGGTTCTGTTC
PRIMER_LEFT_1=487,22
PRIMER_RIGHT_1=736,22
PRIMER_LEFT_1_TM=60.068
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.0864
PRIMER_LEFT_2_PENALTY=0.067516
PRIMER_RIGHT_2_PENALTY=0.018835
PRIMER_LEFT_2_SEQUENCE=TTATCAACTGGAGGAGGAGGAA
PRIMER_RIGHT_2_SEQUENCE=GTTTCGTCCTTTGGTTCTGTTC
PRIMER_LEFT_2=488,22
PRIMER_RIGHT_2=736,22
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=60.019
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.1014
PRIMER_LEFT_3_PENALTY=0.095831
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=ACGACACCGTGTTGATAGTCAG
PRIMER_RIGHT_3_SEQUENCE=TCTTGGGACACTCTACCTCCAT
PRIMER_LEFT_3=459,22
PRIMER_RIGHT_3=716,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=0.1147
PRIMER_LEFT_4_PENALTY=0.095831
PRIMER_RIGHT_4_PENALTY=0.018835
PRIMER_LEFT_4_SEQUENCE=ACGACACCGTGTTGATAGTCAG
PRIMER_RIGHT_4_SEQUENCE=GTTTCGTCCTTTGGTTCTGTTC
PRIMER_LEFT_4=459,22
PRIMER_RIGHT_4=736,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=60.019
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_05722 Ps_contig_mira-and-tgicl-ass_franssen_39625
SEQUENCE=GGCCATGGTCGTGGTGGAACAGTTCCCTCCCTCCCAACTAGGTTTTTCCCTTTCTTTTCCNCATTTATGTCTTCCATTCAACAACCTAGTATAGGTCGTGGTCGAGGTTTTGGTCCATTACCACCTCAATCTGAAAATGATAAGCAGCAAGAACAACAACCTGATTCTGTACCTAAGAAACCCGTTTTCTTCAGAAGAGAGGATAATGTTTCTCAGACAGAAACAAATGAAGTATTGCCTCACAAAAAACCCATTTTTACTAGAACGGAGGATGTTAAACCAATAGATTTATCTGGTGATAATGAGAGTGATAGTATGTTCTCAATGAGCTTGTCAGGGGTGTTGCCTGGAACTGGGAGGGGGAAGCCCGTGGAAGAGGCGGGTCGAGAAGCTCCTCAAGTTACAATAGAGAATAGGCATATCCGCAGTCGTCGAACTACTGGTGATGCTGCTTCTGAGAATGTTCCTAAGAGACAGCCAATGCTAAGCCAGAATGATGGTGATGGAAGTGGTAGTGGAAGAGGAAGAGAACCTAGGGAGAGAGGTGGGTTTGTTCGTGGCCGAGGTAGAGGACGTGGTAGAGGAAGAGGTGTTGGAAGGGGAGGCTTTAGAGGGGCGGGTGCAGATGATAGGATGGGTCAGATTCAAGATTCAGCGCGTTCCAATGCTGCTGGGCTATACACTGGAGATAATGCAGATGGTGAAAAGTTAGCTCAGAAGGTTGGGCCTGAGATAATGAATCAGTTGACGGAAGAGTTCGAGGAGATTGTTAGTAGAGTTTTACCCTCTCCGTTGGAAGATGAATATGTGGAAGCATTAGACATTAATTGCGCTATTGAGTTTGAGCCGGAATATATTATGGAGTTTGATAGCAATCCAGATATTGATGAGAAAGAACCAATTCCACTTCGGGATGCACTAGAGAAAATGAAACCATTCTTGATGAAATACGAGGGGATTAAAAGTCAAGAAGAGTGGGAGGAAATAATGGAAGAGACAATGGAACGAGTTCCATTATTGAAAAAGATTGTTGATCACTACAGCGGACCTGATAGAGTAACCGCAAAGAAGCAAACAGAAGAGTTAGAGAGAGTTGCTAAAACTCTTCCTGGAAGTGCGCCATCTTCTGTGAAGGATTTCACTAACCGAGCTGTTATCTCTCTTCAGAGCAACCCAGGCTGGGGATTTGACAAGAAATGCCAATTCATGGATAAGCTAGTTTTTGAAGTGTCTCAGCATCACAAATAATCTAATAAGTCTTTCAAGATTAAACATGGTGGGTTTCATAGTATACAATTTTATTTACTTGTGGACAAGTCATGCTTTCTTTCATTTGTTTTTGATTAAAGAAACTGCTATAACTCTTGTAATAATATGAATGATAGCTATCTTGTAGCATTGATGCGAGAATTTTCCATTTTGTTTTAGATTCTTGGCTATAAAGATAGAGCCCTGTCTGTAAAGTTAGAGCTTGTCTATTTTAGTTTTTCCCATAATAATAGACAGAAGATGCAGAGGAGAAGAAATGGGGTGATGCACACATTGAATGCTTTTAATGTTGGCATGTATTACTTTGCTCTTATAATATGAGTTGAATATTACTTTCTATTATTTTTCGACTGAAA
TARGET=24,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.0094
PRIMER_LEFT_PENALTY=3.880856
PRIMER_RIGHT_PENALTY=0.128591
PRIMER_LEFT_SEQUENCE=CATGGTCGTGGTGGAACAGT
PRIMER_RIGHT_SEQUENCE=TTGGTTTAACATCCTCCGTTCT
PRIMER_LEFT=3,20
PRIMER_RIGHT=282,22
PRIMER_LEFT_TM=61.881
PRIMER_RIGHT_TM=59.871
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=4.3438
PRIMER_LEFT_1_PENALTY=3.880856
PRIMER_RIGHT_1_PENALTY=0.462913
PRIMER_LEFT_1_SEQUENCE=CATGGTCGTGGTGGAACAGT
PRIMER_RIGHT_1_SEQUENCE=ATTGAGGTGGTAATGGACCAAA
PRIMER_LEFT_1=3,20
PRIMER_RIGHT_1=129,22
PRIMER_LEFT_1_TM=61.881
PRIMER_RIGHT_1_TM=60.463
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=4.4517
PRIMER_LEFT_2_PENALTY=3.880856
PRIMER_RIGHT_2_PENALTY=0.570821
PRIMER_LEFT_2_SEQUENCE=CATGGTCGTGGTGGAACAGT
PRIMER_RIGHT_2_SEQUENCE=TTCAGATTGAGGTGGTAATGGA
PRIMER_LEFT_2=3,20
PRIMER_RIGHT_2=134,22
PRIMER_LEFT_2_TM=61.881
PRIMER_RIGHT_2_TM=59.429
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=4.4907
PRIMER_LEFT_3_PENALTY=3.880856
PRIMER_RIGHT_3_PENALTY=0.609876
PRIMER_LEFT_3_SEQUENCE=CATGGTCGTGGTGGAACAGT
PRIMER_RIGHT_3_SEQUENCE=CAGATTGAGGTGGTAATGGACC
PRIMER_LEFT_3=3,20
PRIMER_RIGHT_3=132,22
PRIMER_LEFT_3_TM=61.881
PRIMER_RIGHT_3_TM=60.610
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=130
PRIMER_PAIR_PENALTY_4=4.5953
PRIMER_LEFT_4_PENALTY=3.880856
PRIMER_RIGHT_4_PENALTY=0.714453
PRIMER_LEFT_4_SEQUENCE=CATGGTCGTGGTGGAACAGT
PRIMER_RIGHT_4_SEQUENCE=AATCAGGTTGTTGTTCTTGCTG
PRIMER_LEFT_4=3,20
PRIMER_RIGHT_4=165,22
PRIMER_LEFT_4_TM=61.881
PRIMER_RIGHT_4_TM=59.286
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=163
=
PRIMER_SEQUENCE_ID=SSR_05723 Ps_contig_mira-and-tgicl-ass_franssen_39630
SEQUENCE=TTAAAATACTCTTCTACCACGCAAAAAACTACCCACTAAGATGTTGTAACACACAGAATGCCCTTTCACGATCAACACCATTCATGTAAGCAGCAGACTCCAAATCAGAACCAAAATCTTCATCCAAAATAGAATCAATCTTCATCTCCTTCCTCCATTTGAGTGTCTTCTTCAACATCTCAAAAGCCTCATTCACCTTAAAATCTCTAGCCCTCAAAAACTTCAACAAAACCACATCAATTCCTTCATCTCCTTTGCTTGGCAAAAGTGACACACCCCACAAAGATAAATCTTTCTCCTCCTCCACTTCAACCCCTTTCTCGTTCTCCCCCTCAACATTCTCTTCTTCCTTCACAACTTTCTCACCCTCTTCACCACCTTCTGGTAAAGCTTCAACCTTTTTAGTCTCTTCTTCTTCTTTTTTCTCAAAAAGAGTGTTCCCTAAAATCGCTTCCTCAACCTTAGACTTGAACTCAACCAAAGCCTTTTTCTCAAACTCTTTAAGATCTGAAAGAAAGTTGCTTTCTTCTTTATAAGAAGAGCATTTCTCAACTGCCTTTGCGGGTTTTGATTCATCCAATGTTTCCTTTGCTTCAACATCAAGAACTTTACCTTCTTCAACAACAACCTCAACTTTCTGGGTGGTTTCTTCTTGAGCCATAGTTTCAGCAGTCATGTTTTTCTACTCACTGAAAACACAGCAAAAACAACAAAATAAGACCAAAAAATAAAACTTAGTGACAAACCCAGAAATTTAAAAGTGACAACAAACAAAACAAATAGAAAAAAAACNACAAACAAAATT
TARGET=408,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1579
PRIMER_LEFT_PENALTY=0.145769
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=AACTTTCTCACCCTCTTCACCA
PRIMER_RIGHT_SEQUENCE=AAGAAACCACCCAGAAAGTTGA
PRIMER_LEFT=355,22
PRIMER_RIGHT=650,22
PRIMER_LEFT_TM=60.146
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=0.1579
PRIMER_LEFT_1_PENALTY=0.145769
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=AACTTTCTCACCCTCTTCACCA
PRIMER_RIGHT_1_SEQUENCE=GAAGAAACCACCCAGAAAGTTG
PRIMER_LEFT_1=355,22
PRIMER_RIGHT_1=651,22
PRIMER_LEFT_1_TM=60.146
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=297
PRIMER_PAIR_PENALTY_2=0.1767
PRIMER_LEFT_2_PENALTY=0.164560
PRIMER_RIGHT_2_PENALTY=0.012153
PRIMER_LEFT_2_SEQUENCE=CTTCACCACCTTCTGGTAAAGC
PRIMER_RIGHT_2_SEQUENCE=AAGAAACCACCCAGAAAGTTGA
PRIMER_LEFT_2=369,22
PRIMER_RIGHT_2=650,22
PRIMER_LEFT_2_TM=60.165
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.1767
PRIMER_LEFT_3_PENALTY=0.164560
PRIMER_RIGHT_3_PENALTY=0.012153
PRIMER_LEFT_3_SEQUENCE=CTTCACCACCTTCTGGTAAAGC
PRIMER_RIGHT_3_SEQUENCE=GAAGAAACCACCCAGAAAGTTG
PRIMER_LEFT_3=369,22
PRIMER_RIGHT_3=651,22
PRIMER_LEFT_3_TM=60.165
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.2827
PRIMER_LEFT_4_PENALTY=0.145769
PRIMER_RIGHT_4_PENALTY=0.136881
PRIMER_LEFT_4_SEQUENCE=AACTTTCTCACCCTCTTCACCA
PRIMER_RIGHT_4_SEQUENCE=CAAGAAGAAACCACCCAGAAAG
PRIMER_LEFT_4=355,22
PRIMER_RIGHT_4=654,22
PRIMER_LEFT_4_TM=60.146
PRIMER_RIGHT_4_TM=60.137
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=300
=
PRIMER_SEQUENCE_ID=SSR_05724 Ps_contig_mira-and-tgicl-ass_franssen_39635
SEQUENCE=AGTACGCGGGACAAGATATCTTTAGTGTTAACTTTGAAATATCTTTTATAATCCTAAAATCTAGCTTTTTGTCTTTTGTCAATGGCGTCTGGAGATGTTGAGTACCGGTGCTTCGTCGGCGGTCTTGCATGGGCCACCGACAGCGAAGCTCTCGAGAAAGCTTTCTCTTCTTACGGCGACATCGTTGATTCGAAGATCATTAACGATCGTGAGACTGGAAGGTCAAGAGGTTTCGGATTTGTGACATTCGCTAATGAGAAATCGATGAGAGATGCTATTGAAGGGATGAACGGCCAGAACATGGACGGGCGTAACATTACCGTTAACGAAGCTCAAAACCGTAACAGTGGTGGTGGTGGTGGAGGTGGCGGCTATGGTGGTCGTCGTGAAGGTGGAGGTGGAGGCGGAGGCTATGGTGGTCGCCGTGAAGGTGGAGGAGGAGGCTATGGTGGTGGAGGTGGTTATGGTGGTGGTGGTAGGGACCGGGGATATGGTAATGACGGTGGTGACCGCTATTCCCGTGGTGGCGGTGGAGGTGGTGGAAACTGGAGGGAGTAGTGTGTTTTAGGTTGAATTTGAGAACTAATAATGTTTAGTTGTTAAATTAGATGTTGGGATCTAACGTTCTGGTTTATGGTATCTGTTTTGGTTATGGTTTCCGGTTTCGTTTATGTATGCCTCTGAATCTGGTTGTGTATAAACGATCGGTTGAAAGGATAATGAGATACTATTTAATGAACTAATTTCTACTTATTTCTAGNNACT
TARGET=347,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1321
PRIMER_LEFT_PENALTY=0.129818
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=AGAGGTTTCGGATTTGTGACAT
PRIMER_RIGHT_SEQUENCE=ATAACCACCTCCACCACCATAG
PRIMER_LEFT=225,22
PRIMER_RIGHT=464,22
PRIMER_LEFT_TM=59.870
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.1346
PRIMER_LEFT_1_PENALTY=0.132262
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=CATTAACGATCGTGAGACTGGA
PRIMER_RIGHT_1_SEQUENCE=ATAACCACCTCCACCACCATAG
PRIMER_LEFT_1=197,22
PRIMER_RIGHT_1=464,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=0.2308
PRIMER_LEFT_2_PENALTY=0.228464
PRIMER_RIGHT_2_PENALTY=0.002310
PRIMER_LEFT_2_SEQUENCE=GAGGTTTCGGATTTGTGACATT
PRIMER_RIGHT_2_SEQUENCE=ATAACCACCTCCACCACCATAG
PRIMER_LEFT_2=226,22
PRIMER_RIGHT_2=464,22
PRIMER_LEFT_2_TM=60.228
PRIMER_RIGHT_2_TM=59.998
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.2308
PRIMER_LEFT_3_PENALTY=0.228464
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=AGGTTTCGGATTTGTGACATTC
PRIMER_RIGHT_3_SEQUENCE=ATAACCACCTCCACCACCATAG
PRIMER_LEFT_3=227,22
PRIMER_RIGHT_3=464,22
PRIMER_LEFT_3_TM=60.228
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.2901
PRIMER_LEFT_4_PENALTY=0.287765
PRIMER_RIGHT_4_PENALTY=0.002310
PRIMER_LEFT_4_SEQUENCE=GTGAGACTGGAAGGTCAAGAGG
PRIMER_RIGHT_4_SEQUENCE=ATAACCACCTCCACCACCATAG
PRIMER_LEFT_4=208,22
PRIMER_RIGHT_4=464,22
PRIMER_LEFT_4_TM=60.288
PRIMER_RIGHT_4_TM=59.998
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_05725 Ps_contig_mira-and-tgicl-ass_franssen_39635
SEQUENCE=AGTACGCGGGACAAGATATCTTTAGTGTTAACTTTGAAATATCTTTTATAATCCTAAAATCTAGCTTTTTGTCTTTTGTCAATGGCGTCTGGAGATGTTGAGTACCGGTGCTTCGTCGGCGGTCTTGCATGGGCCACCGACAGCGAAGCTCTCGAGAAAGCTTTCTCTTCTTACGGCGACATCGTTGATTCGAAGATCATTAACGATCGTGAGACTGGAAGGTCAAGAGGTTTCGGATTTGTGACATTCGCTAATGAGAAATCGATGAGAGATGCTATTGAAGGGATGAACGGCCAGAACATGGACGGGCGTAACATTACCGTTAACGAAGCTCAAAACCGTAACAGTGGTGGTGGTGGTGGAGGTGGCGGCTATGGTGGTCGTCGTGAAGGTGGAGGTGGAGGCGGAGGCTATGGTGGTCGCCGTGAAGGTGGAGGAGGAGGCTATGGTGGTGGAGGTGGTTATGGTGGTGGTGGTAGGGACCGGGGATATGGTAATGACGGTGGTGACCGCTATTCCCGTGGTGGCGGTGGAGGTGGTGGAAACTGGAGGGAGTAGTGTGTTTTAGGTTGAATTTGAGAACTAATAATGTTTAGTTGTTAAATTAGATGTTGGGATCTAACGTTCTGGTTTATGGTATCTGTTTTGGTTATGGTTTCCGGTTTCGTTTATGTATGCCTCTGAATCTGGTTGTGTATAAACGATCGGTTGAAAGGATAATGAGATACTATTTAATGAACTAATTTCTACTTATTTCTAGNNACT
TARGET=465,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0334
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.031122
PRIMER_LEFT_SEQUENCE=CTATGGTGGTGGAGGTGGTTAT
PRIMER_RIGHT_SEQUENCE=CACTACTCCCTCCAGTTTCCAC
PRIMER_LEFT=443,22
PRIMER_RIGHT=559,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=117
PRIMER_PAIR_PENALTY_1=0.0334
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.031122
PRIMER_LEFT_1_SEQUENCE=CTATGGTGGTGGAGGTGGTTAT
PRIMER_RIGHT_1_SEQUENCE=CACACTACTCCCTCCAGTTTCC
PRIMER_LEFT_1=443,22
PRIMER_RIGHT_1=561,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=119
PRIMER_PAIR_PENALTY_2=0.0334
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.031122
PRIMER_LEFT_2_SEQUENCE=CTATGGTGGTGGAGGTGGTTAT
PRIMER_RIGHT_2_SEQUENCE=ACACTACTCCCTCCAGTTTCCA
PRIMER_LEFT_2=443,22
PRIMER_RIGHT_2=560,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=60.031
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=0.1107
PRIMER_LEFT_3_PENALTY=0.002310
PRIMER_RIGHT_3_PENALTY=0.108385
PRIMER_LEFT_3_SEQUENCE=CTATGGTGGTGGAGGTGGTTAT
PRIMER_RIGHT_3_SEQUENCE=TACATAAACGAAACCGGAAACC
PRIMER_LEFT_3=443,22
PRIMER_RIGHT_3=674,22
PRIMER_LEFT_3_TM=59.998
PRIMER_RIGHT_3_TM=60.108
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=232
PRIMER_PAIR_PENALTY_4=0.1266
PRIMER_LEFT_4_PENALTY=0.095496
PRIMER_RIGHT_4_PENALTY=0.031122
PRIMER_LEFT_4_SEQUENCE=AGCTCAAAACCGTAACAGTGGT
PRIMER_RIGHT_4_SEQUENCE=CACACTACTCCCTCCAGTTTCC
PRIMER_LEFT_4=329,22
PRIMER_RIGHT_4=561,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=60.031
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_05726 Ps_contig_mira-and-tgicl-ass_franssen_39636
SEQUENCE=TTGTCGGGGAATNAATATAACCGTTAAGTAAGAACGATTGTAGTAGTACTAGTGTAAAACTCGTCAAGCTCGCTCGCTCGCTCGCATCCATTCTATATAATAAACCTCTTTCTATCTCCTCCACTCCAACCTTCACACAGGAAACACAATCGACCAAACGGTTGGGGAATCTGTGATCTCGTGAGACATGATGAATTCTAGAAGAGACACCCGAAACAACAGAGTTGCTCTTTTTGATGGTATTGAGGAGGGAGGCATCAGAGCATCATCTCTTTACTCCTCAGGTTCTTCCCATGAAATTGATGAACATGATAATGAACAAGCAATGGACGGATTGCAAGATAGAGTTAATTTGCTGAAAAGACTAGAGATAAAGTTTTAATGGGTGTGATTGCAACAGTTGTCAGGTGATATACATGAAGAGGTGGATAATCATAACCGGATGCTGGATAATATGGGCAATGATATGGATTCTTCAAGGGGAGTCCTCTCAGGAACTATGGACAAATTCAAAATGGTATTTGAGACAAAATCGAGCCGAAGAATGTTCTCGCTCGTAGCATCATTTGTTGTTATTTTTCTTATCATTTACTATCTAACTAGGTAACTGGTCTTTCATTCTGTCAGCTTGATGTAAGATCTGATGAAAGCAGGCTACTACTTGCTCTGTTACTGTAATTAATGTCACTACTTGTAAGATGTTCTGTTATGTTTGTATAAAGATTTTTACTTTTTAATATAATTTTTCTATTCCCTTNCTGTTAAAT
TARGET=68,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4832
PRIMER_LEFT_PENALTY=0.409016
PRIMER_RIGHT_PENALTY=0.074231
PRIMER_LEFT_SEQUENCE=GTCGGGGAATNAATATAACCGT
PRIMER_RIGHT_SEQUENCE=AACTCTGTTGTTTCGGGTGTCT
PRIMER_LEFT=2,22
PRIMER_RIGHT=225,22
PRIMER_LEFT_TM=59.591
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.3000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.75
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.5115
PRIMER_LEFT_1_PENALTY=0.409016
PRIMER_RIGHT_1_PENALTY=0.102490
PRIMER_LEFT_1_SEQUENCE=GTCGGGGAATNAATATAACCGT
PRIMER_RIGHT_1_SEQUENCE=TGGGAAGAACCTGAGGAGTAAA
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=293,22
PRIMER_LEFT_1_TM=59.591
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.3000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.5857
PRIMER_LEFT_2_PENALTY=0.409016
PRIMER_RIGHT_2_PENALTY=0.176730
PRIMER_LEFT_2_SEQUENCE=GTCGGGGAATNAATATAACCGT
PRIMER_RIGHT_2_SEQUENCE=CCCTCCTCAATACCATCAAAAA
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=251,22
PRIMER_LEFT_2_TM=59.591
PRIMER_RIGHT_2_TM=60.177
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.3000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.7208
PRIMER_LEFT_3_PENALTY=0.409016
PRIMER_RIGHT_3_PENALTY=0.311784
PRIMER_LEFT_3_SEQUENCE=GTCGGGGAATNAATATAACCGT
PRIMER_RIGHT_3_SEQUENCE=CTCCCTCCTCAATACCATCAAA
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=59.591
PRIMER_RIGHT_3_TM=60.312
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=252
PRIMER_PAIR_PENALTY_4=0.7869
PRIMER_LEFT_4_PENALTY=0.409016
PRIMER_RIGHT_4_PENALTY=0.377925
PRIMER_LEFT_4_SEQUENCE=GTCGGGGAATNAATATAACCGT
PRIMER_RIGHT_4_SEQUENCE=AAGAGATGATGCTCTGATGCCT
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=273,22
PRIMER_LEFT_4_TM=59.591
PRIMER_RIGHT_4_TM=60.378
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.3000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_05727 Ps_contig_mira-and-tgicl-ass_franssen_39644
SEQUENCE=TTATCAAGCAATAAATTAGTACATTTGCATTCAATTTACAACTATACAGTGATATGCACCAATTCTATTGAACCTTATTGACAAGCAACTATCTATGTAGCAATAATGTGATTACCTCACACTACACAAATAACTTCAAAATCACTCGAATCCCACCCTTTTAACCATTCAAGAAGAAAAGAAAACCCAACCCTATCAGTTTTGTCAATATTATTACAACACTTCCAATATCATTACAGGTTGAAATAATACTAAAATAGAGTCCCTTTTCTTTGGTTCTCCTTCCTGCAAAATTGTCAATCATGCATATATATATTGCACGCAAAGAATGAAATATGATACATTATTCTTATTCTTATTATATCTTTATGCTGCAGCAGAGTGCTTATCCTCATAATCATCAAGATTAAGAGCATGTAGCAACTTTGGCCAAGATTCTGGGATTCCTCCAGGCAGGTTAAACTCTAATAATTTCCCTCTATTGCGGTAAAACTCTTCCACAGGCCGACTCAATTCATTGTATATCCGAAGCCTTTCTTTGACAACTACTTCAGTATCATCTGACCGAGTATTGAGCTTCGACATACAATTGACAGGAGGAAGAAGCGGAGCCATGACAATTCCAGGGCTCCCATTCTCAGCCTTGATGTCAATGGAAGCAACATTAAAATTTCCTCCGCACTGATTACAAATTCTCCTACCAAGGCATTTCTCAAGCAGTACATCTTCTCGAAGCCTTAGATTGATAACCAAGTCAATATCAGTTACCCCTTCCAATATTTCTGCTTGCTTAATTGTTCGAGGAAAACCATCAAGAATAAATCCCAGATCACCTTTAGCTTCCCCGGCAACAAGTCTCTTTGACAATAAATTTATAATGATTTCATCCGACACCAGTTGACCTTGATTTACAATTTCTGATAGCCGTGAAGAGAGTGGACCGGAGGAGGCGAGCTCTTCGCGAACGAGATCACCGGTGGCGATGTGAGGGACGCCGAGGAGATTGGACAGACGGCTAGCGTAGGTCCCTTTCCCGACGCCAGGACAACCGAGGAAGACCCACCGTACGTGACTGTTCTTCTGATCTTCTCCGCGCGTAGGAACAAGACGGAATGGCGCGTGGGAGTTGAGCTCGGAGGATAGATATCGGTTCGCGATGGAAGAGAGGAAAGACGCGCGCGTAGAAAGGCGGGTGACGGCCGCCATGGGATTGTGCAGGAACGAGACTCACGCCGACCAACAAAGCAATGATTGATCACTTCGTTTGTTTAACNTGTAAGTTGTGCTTTGAAT
TARGET=307,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1559
PRIMER_LEFT_PENALTY=0.018372
PRIMER_RIGHT_PENALTY=0.137550
PRIMER_LEFT_SEQUENCE=GTCCCTTTTCTTTGGTTCTCCT
PRIMER_RIGHT_SEQUENCE=ATACAATGAATTGAGTCGGCCT
PRIMER_LEFT=261,22
PRIMER_RIGHT=522,22
PRIMER_LEFT_TM=59.982
PRIMER_RIGHT_TM=59.862
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=0.1559
PRIMER_LEFT_1_PENALTY=0.018372
PRIMER_RIGHT_1_PENALTY=0.137550
PRIMER_LEFT_1_SEQUENCE=AGTCCCTTTTCTTTGGTTCTCC
PRIMER_RIGHT_1_SEQUENCE=ATACAATGAATTGAGTCGGCCT
PRIMER_LEFT_1=260,22
PRIMER_RIGHT_1=522,22
PRIMER_LEFT_1_TM=59.982
PRIMER_RIGHT_1_TM=59.862
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.3784
PRIMER_LEFT_2_PENALTY=0.018372
PRIMER_RIGHT_2_PENALTY=0.360050
PRIMER_LEFT_2_SEQUENCE=GTCCCTTTTCTTTGGTTCTCCT
PRIMER_RIGHT_2_SEQUENCE=AGTTTAACCTGCCTGGAGGAAT
PRIMER_LEFT_2=261,22
PRIMER_RIGHT_2=463,22
PRIMER_LEFT_2_TM=59.982
PRIMER_RIGHT_2_TM=60.360
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.3784
PRIMER_LEFT_3_PENALTY=0.018372
PRIMER_RIGHT_3_PENALTY=0.360050
PRIMER_LEFT_3_SEQUENCE=AGTCCCTTTTCTTTGGTTCTCC
PRIMER_RIGHT_3_SEQUENCE=AGTTTAACCTGCCTGGAGGAAT
PRIMER_LEFT_3=260,22
PRIMER_RIGHT_3=463,22
PRIMER_LEFT_3_TM=59.982
PRIMER_RIGHT_3_TM=60.360
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.4020
PRIMER_LEFT_4_PENALTY=0.264444
PRIMER_RIGHT_4_PENALTY=0.137550
PRIMER_LEFT_4_SEQUENCE=TTTTCTTTGGTTCTCCTTCCTG
PRIMER_RIGHT_4_SEQUENCE=ATACAATGAATTGAGTCGGCCT
PRIMER_LEFT_4=266,22
PRIMER_RIGHT_4=522,22
PRIMER_LEFT_4_TM=59.736
PRIMER_RIGHT_4_TM=59.862
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_05728 Ps_contig_mira-and-tgicl-ass_franssen_39654
SEQUENCE=ATTAGACTTTGAGACAAAATTGAAATTGTTAACCATTAACATAATTCATTGATATTCTAATGTACATAAAGGCTATAGCTAGCAACCCATAGAAAAAAAATTGTTACACTCTCCCATAATATCCAAGCCGTTAGCTATTTACACCCCACCATGAATCAAAGGAAGAAGGCAAAAACAAAAACCTAAAGAAGAAAGAACCAATCTTGTTCTATTGCCAAAGAACCAAGTTTGAACTTCTAAATCTTATAAAGACCTCAACTCAACTAACTAACTAGCTATTTAGCATGTGTTTGAATCAACTATGAATAAGGCTAACAAAGTTTAGAGCTCTCTGATCGTGAATTATAGAAGCTCCAATGTATAGCTTCTGATCGAATCTCGAAATTTTAGTATTTTCCAAACACAAAGACAATTACTCATGCCAACATAGAAGCATGACCACACACCTTCTTATGATGTATAGCCTAGCTCTTTGTTCCTTAAGAACACCACCAAGTCCTCTACTTGATGATGAAGATAGTCTTGATCTCTTTGAGCTTCCAATAGAACCCATCTTCATATTATGAGTGAAATAGTATAGAAACTCTTGGAAAACAAAAAGGGTTGTTTTTCTTTAGATTTTAGAACTTGAGTTGTGTGTGAAAGACTTCCTCCGAC
TARGET=262,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4838
PRIMER_LEFT_PENALTY=0.540037
PRIMER_RIGHT_PENALTY=0.943780
PRIMER_LEFT_SEQUENCE=ACCATGAATCAAAGGAAGAAGG
PRIMER_RIGHT_SEQUENCE=TGTGGTCATGCTTCTATGTTGG
PRIMER_LEFT=147,22
PRIMER_RIGHT=442,22
PRIMER_LEFT_TM=59.460
PRIMER_RIGHT_TM=60.944
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=1.7399
PRIMER_LEFT_1_PENALTY=1.082156
PRIMER_RIGHT_1_PENALTY=0.657742
PRIMER_LEFT_1_SEQUENCE=CCACCATGAATCAAAGGAAGA
PRIMER_RIGHT_1_SEQUENCE=CATGCTTCTATGTTGGCATGAG
PRIMER_LEFT_1=145,21
PRIMER_RIGHT_1=436,22
PRIMER_LEFT_1_TM=59.918
PRIMER_RIGHT_1_TM=60.658
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=1.8955
PRIMER_LEFT_2_PENALTY=1.455150
PRIMER_RIGHT_2_PENALTY=0.440316
PRIMER_LEFT_2_SEQUENCE=CATGAATCAAAGGAAGAAGGCA
PRIMER_RIGHT_2_SEQUENCE=GGTGTGTGGTCATGCTTCTATG
PRIMER_LEFT_2=149,22
PRIMER_RIGHT_2=446,22
PRIMER_LEFT_2_TM=61.455
PRIMER_RIGHT_2_TM=60.440
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=1.9641
PRIMER_LEFT_3_PENALTY=1.306406
PRIMER_RIGHT_3_PENALTY=0.657742
PRIMER_LEFT_3_SEQUENCE=CACCATGAATCAAAGGAAGAAG
PRIMER_RIGHT_3_SEQUENCE=CATGCTTCTATGTTGGCATGAG
PRIMER_LEFT_3=146,22
PRIMER_RIGHT_3=436,22
PRIMER_LEFT_3_TM=58.694
PRIMER_RIGHT_3_TM=60.658
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=2.0259
PRIMER_LEFT_4_PENALTY=1.082156
PRIMER_RIGHT_4_PENALTY=0.943780
PRIMER_LEFT_4_SEQUENCE=CCACCATGAATCAAAGGAAGA
PRIMER_RIGHT_4_SEQUENCE=TGTGGTCATGCTTCTATGTTGG
PRIMER_LEFT_4=145,21
PRIMER_RIGHT_4=442,22
PRIMER_LEFT_4_TM=59.918
PRIMER_RIGHT_4_TM=60.944
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_05729 Ps_contig_mira-and-tgicl-ass_franssen_39662
SEQUENCE=TTGGTTGATTTTGCACACTCTCAAGGGTTTGTCTTGCAGGAGATAAAACCATCTTGTTTTACGTTATCGCCTTCGAATAAGATTAAATACATTGGTTCGTATAGCGAACAAGTTTTTGATGACAGAAAATCGTGTTTCACCGTGTTTAAGTCGTGTCTTAAGGCCATTGTGACTTGTAAAGAGACAGAATCTCTTAAGCAGCAGCAGCATCATTTCAAAGAAAAGATGTGTCTAGATGGTTCCAGTTATCAACATGCTTTTGACAGAAGAAAAGCAGTTTGTTTCTGAAACCAT
TARGET=197,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1766
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=1.161374
PRIMER_LEFT_SEQUENCE=TGACAGAAAATCGTGTTTCACC
PRIMER_RIGHT_SEQUENCE=GCATGTTGATAACTGGAACCAT
PRIMER_LEFT=119,22
PRIMER_RIGHT=256,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=58.839
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=138
PRIMER_PAIR_PENALTY_1=1.2222
PRIMER_LEFT_1_PENALTY=0.060824
PRIMER_RIGHT_1_PENALTY=1.161374
PRIMER_LEFT_1_SEQUENCE=AGTCGTGTCTTAAGGCCATTGT
PRIMER_RIGHT_1_SEQUENCE=GCATGTTGATAACTGGAACCAT
PRIMER_LEFT_1=148,22
PRIMER_RIGHT_1=256,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=58.839
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=1.2354
PRIMER_LEFT_2_PENALTY=0.073989
PRIMER_RIGHT_2_PENALTY=1.161374
PRIMER_LEFT_2_SEQUENCE=TTCACCGTGTTTAAGTCGTGTC
PRIMER_RIGHT_2_SEQUENCE=GCATGTTGATAACTGGAACCAT
PRIMER_LEFT_2=135,22
PRIMER_RIGHT_2=256,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=58.839
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=1.3154
PRIMER_LEFT_3_PENALTY=0.154023
PRIMER_RIGHT_3_PENALTY=1.161374
PRIMER_LEFT_3_SEQUENCE=TTGGTTGATTTTGCACACTCTC
PRIMER_RIGHT_3_SEQUENCE=GCATGTTGATAACTGGAACCAT
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=256,22
PRIMER_LEFT_3_TM=60.154
PRIMER_RIGHT_3_TM=58.839
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=1.3706
PRIMER_LEFT_4_PENALTY=0.209271
PRIMER_RIGHT_4_PENALTY=1.161374
PRIMER_LEFT_4_SEQUENCE=GCGAACAAGTTTTTGATGACAG
PRIMER_RIGHT_4_SEQUENCE=GCATGTTGATAACTGGAACCAT
PRIMER_LEFT_4=103,22
PRIMER_RIGHT_4=256,22
PRIMER_LEFT_4_TM=59.791
PRIMER_RIGHT_4_TM=58.839
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_05730 Ps_contig_mira-and-tgicl-ass_franssen_39664
SEQUENCE=ATGAATGAATGAATGTGTGAAGTTCATTGAATAAATGACTACACTACATCACATTCATATTTATTAACCAGACAATACCTRAAACAATCACGYTTTTTATTAACTTCTGCCAAAATAATACTACAACTTTTTTTTCTGTAATGAAATTGAGTTCTGGAAAGAGTACACGCTGTCAAAGTTGTTTGAGTGACATGTTTATTGAATAAAATACTCACAAATAGTTTACATAAGATTAGAATGAATTGATCACTACTATACAGTTTTGAGAAATGAAATACACGAGGAATGCGTTATGTACGCAGAACAGGGAAAGGGAATCAAGAATCGGTAGTGGAATCGATGGGAAGATGTAGCGAAGGTTGATGATCGTTCTTGCTCTTCTCCAAAGATATCAGCAGAATATCCTCATCAGTACCAAAGGCCTTGCGAGCTTCTTCTAAAGACTTCGACGATATCCTTTTATGTTTCCTAGAGCTAGCAGGATCTCTAATCTCAAACATATCTGCTCCAGCATCAAAGCCACATATCAACACATAGTGACCTGTGTAGTCAGAGTTATTGCTGAAGACGCCAGCGACATGAACATCTTGCCATGCATTACTCAATTTATTGTGGTCAACTAATGCAATGGCTAAATATTTCCCAGACAATATGAGAAAGGAAATCTCCTCTTCGCTAAGTGACCTGCACTGGATATCAATTCCAGCTTCCATTGCATCCTGAAACAGTGTATCCACTCTTACTAGATCATTAGGCAGTTCCTCCTTGTAAAAGGATTCACCACAATAGTTTGGGTTTGCACCAAGTGTCACTGTTAAGTAGGAAAAAGTAACAGAAAATCTTTGTAATAAATACGCCAAATCAACAGTCCAAATGCTATTTGTGCAGCATAGGTCAGCCAGAGCTTGAATATCAAAGTTGTTGACACCAATAGTTTTCAGAACCATTACCACACAAGCAAGGCCGCAATCCCATGTATATATTTGATTTATGTGTGGTACTTCAATGAAAAGTGAACGTCGTAATGACGGAGGATTGGAATCTATGTTACATGATAAATTCTGAAACAGGTATGGTTCAATCACCTTCAGATTTTCTCCTTTTATTTCTCTTTCATCTCCCGTTTTAAGAATCTTGTTGAATAGCAGGCATATGGGCCACATCAATGACTAAATTGAATGTGTACAATGTTTACTTTCAAAAAAGGTTCAGGAAATCAAAAGAGTTCAGAGAAGGTAGATGGGCTGTCCTTGTAGTGTATGTGGAGTACCAACCACGTTGATAGAGTACAAGGGAGAGAGGTGAATACAGCGCGTGTTGAAACGGAGACGGAGACAGACAGGTTGTTCAAGTATCACGTCGGTTGGTGGCTTCTTTGTTGAACTCGATGATTTATGTTCTCTCTCT
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05731 Ps_contig_mira-and-tgicl-ass_franssen_39666
SEQUENCE=TCAANGCAGNGTATCAACGGGGGAGTTCTCTTATTTTCATTCTTTCCTTTGATTCCTTCTTCCAGTGAATCAACAAATGTTTCTTCTCACACCATAACAACTACACATGAATTTTCTCAAGAAATTCAAACGCTTTAACCCTCTAGAACCTTCTATAGCCACTTTCGGTTTCTTTTTCCTCTCTGTTCTTTTCATTGGATGCTTCTTCTACGTTGATTACAAGGGTATACTTCACTCCCATGGAACCAAACTCTTTACCTTTCAATTTTCAACTCTATCTTCTTCTTCTTCTGAGTCATCATCCCCTCCTCCTCCTGTTCAATTTATGACTCCGCAGGGTGATAAATGTGATGTTTTCGACGGAAATTGGGTTTGGGATGAAGCTTATCCACTTTATCACTCCTCAAATTGTTCCTTCTTGGATCAGGGCTTTCGTTGCTCTGAAAATGGTAGACCTGATACTTTCTACACTAAGTGGAGATGGCAACCTAAAGATTGCGACTTGCCGAGATTTGATGCAAGGAAGATGTTGGAAATGTTACGAAACAAGCGGTTAGTTTTTGTTGGTGATTCGATTGGAAGGAACCAATGGGAATCGATGCTTTGTATGCTATCTTCTGCAGTTACTAACAAGACTTCTGTTTATGAGGTGAATGGGACCCCAATCACAAAGCACACAGGATTCTTAGCATTCAGGTTTGCAGATTTCAACTGCACTGTTGAATACTACAGGTCACCGTTTTTAATAGTGCAGGGGCGGCCTCCGCCTGGGGCACCTCGTCGGGTGAGATTGACTTTGAGAGTGGATCAAATGGATTGGACTTCTCATCGGTGGAGAAACGCGGATGTGTTGGTTCTCAATGCCGGACATTGGTGGAATTACCAGAAGACAATCAAAATGGGTTGTTATTTCCAAATAGGGAATGAAGTGAATATGAATATGTCTACAGAAGATGCATTCAGAATATCAGTTGAGACAGTTGTTGATTGGATTGCCCGTGAAGTAAACAGGAACAAAACATATGTCCTTTTCCGAACTTATGCTCCTGTGCATTTCAGGGGTGGTGACTGGAATACTGGTGGTGGTTGTCATTCGGAAACACTACCTGATTTAGGCTTCTTACCGACACAGTCCGACATGCATTTCAGTACCGTCGCTAATGTCTTGCCACAACGCGCAAATAAATCAGAAGTGCTGAATTTGGACTTACTTAATGTCACACAAATGTCAGCACGCAGGAAAGATGGCCACGCATCTATTTACTATATCGGGCCACAGAGAACAGCCTCTATGCAGCGACAAGACTGTAGTCATTGGTGCCTCCCCGGGGTTCCCGATTCATGGAACGAGATTCTTTATTCTCTATTTCTCAAGAAGACTTTTTCAGGTCACAATTCAACTGAAGTTTCTCAAGTTTCACTATAAATAGCTTATACAGTTTGCGTGGAATCGCACATATACAAATCTTCGTCAACTCTACTTAATCATTGGCGAATGGCATGAAGCTGGAAGCTAAATTTGCCATCTTAATTTTTGTGGCCAGTTTCACTAATTGGTGCAACTAACTATCTGTTAGTTTGCCTTGTGTAAAGCAAATAAATTAACATGTTTGGTTTAAGAAAAATGTTTCCTGTTTCTATTTTCTATTTTACTAAGAGTTACAAACACATCCTGTAATTTCACTTTATTTCCTATCTTTGAAGATTTGTAGAGTATAAACTATACAAAATAGTTTTCTTTTACAAATCCTTGAAAATTTGAAATAAAGTGAAAATAAGATATTGTGTTCATAACTATCAGTTAAAATTGAAAATACAAACAGAGAAAAAGTTTTCTTAAATTGAACATGCTAAGTTATTATTTAGATGAATGTGGAATGGTGAATTTGAGGCATAGCAGATAGCTTATAGAAAAATAACAATCTTAGTATCAGAAAGCATACTGCATTTTTTTGGACGGTAAATCTCAAGTTCATTGGTCTTGAAAATATTAGNANAGATGTGCCAAGTTTTTTTGAC
TARGET=278,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2334
PRIMER_LEFT_PENALTY=0.102490
PRIMER_RIGHT_PENALTY=0.130883
PRIMER_LEFT_SEQUENCE=CCTTTGATTCCTTCTTCCAGTG
PRIMER_RIGHT_SEQUENCE=TGCGGAGTCATAAATTGAACAG
PRIMER_LEFT=45,22
PRIMER_RIGHT=335,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=60.131
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.2334
PRIMER_LEFT_1_PENALTY=0.102490
PRIMER_RIGHT_1_PENALTY=0.130883
PRIMER_LEFT_1_SEQUENCE=CCTTTGATTCCTTCTTCCAGTG
PRIMER_RIGHT_1_SEQUENCE=CTGCGGAGTCATAAATTGAACA
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=336,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=60.131
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.2519
PRIMER_LEFT_2_PENALTY=0.134571
PRIMER_RIGHT_2_PENALTY=0.117281
PRIMER_LEFT_2_SEQUENCE=TTCGGTTTCTTTTTCCTCTCTG
PRIMER_RIGHT_2_SEQUENCE=TCTACCATTTTCAGAGCAACGA
PRIMER_LEFT_2=163,22
PRIMER_RIGHT_2=453,22
PRIMER_LEFT_2_TM=59.865
PRIMER_RIGHT_2_TM=59.883
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.2655
PRIMER_LEFT_3_PENALTY=0.134571
PRIMER_RIGHT_3_PENALTY=0.130883
PRIMER_LEFT_3_SEQUENCE=TTCGGTTTCTTTTTCCTCTCTG
PRIMER_RIGHT_3_SEQUENCE=TGCGGAGTCATAAATTGAACAG
PRIMER_LEFT_3=163,22
PRIMER_RIGHT_3=335,22
PRIMER_LEFT_3_TM=59.865
PRIMER_RIGHT_3_TM=60.131
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=173
PRIMER_PAIR_PENALTY_4=0.2655
PRIMER_LEFT_4_PENALTY=0.134571
PRIMER_RIGHT_4_PENALTY=0.130883
PRIMER_LEFT_4_SEQUENCE=TTCGGTTTCTTTTTCCTCTCTG
PRIMER_RIGHT_4_SEQUENCE=CTGCGGAGTCATAAATTGAACA
PRIMER_LEFT_4=163,22
PRIMER_RIGHT_4=336,22
PRIMER_LEFT_4_TM=59.865
PRIMER_RIGHT_4_TM=60.131
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=174
=
PRIMER_SEQUENCE_ID=SSR_05732 Ps_contig_mira-and-tgicl-ass_franssen_39666
SEQUENCE=TCAANGCAGNGTATCAACGGGGGAGTTCTCTTATTTTCATTCTTTCCTTTGATTCCTTCTTCCAGTGAATCAACAAATGTTTCTTCTCACACCATAACAACTACACATGAATTTTCTCAAGAAATTCAAACGCTTTAACCCTCTAGAACCTTCTATAGCCACTTTCGGTTTCTTTTTCCTCTCTGTTCTTTTCATTGGATGCTTCTTCTACGTTGATTACAAGGGTATACTTCACTCCCATGGAACCAAACTCTTTACCTTTCAATTTTCAACTCTATCTTCTTCTTCTTCTGAGTCATCATCCCCTCCTCCTCCTGTTCAATTTATGACTCCGCAGGGTGATAAATGTGATGTTTTCGACGGAAATTGGGTTTGGGATGAAGCTTATCCACTTTATCACTCCTCAAATTGTTCCTTCTTGGATCAGGGCTTTCGTTGCTCTGAAAATGGTAGACCTGATACTTTCTACACTAAGTGGAGATGGCAACCTAAAGATTGCGACTTGCCGAGATTTGATGCAAGGAAGATGTTGGAAATGTTACGAAACAAGCGGTTAGTTTTTGTTGGTGATTCGATTGGAAGGAACCAATGGGAATCGATGCTTTGTATGCTATCTTCTGCAGTTACTAACAAGACTTCTGTTTATGAGGTGAATGGGACCCCAATCACAAAGCACACAGGATTCTTAGCATTCAGGTTTGCAGATTTCAACTGCACTGTTGAATACTACAGGTCACCGTTTTTAATAGTGCAGGGGCGGCCTCCGCCTGGGGCACCTCGTCGGGTGAGATTGACTTTGAGAGTGGATCAAATGGATTGGACTTCTCATCGGTGGAGAAACGCGGATGTGTTGGTTCTCAATGCCGGACATTGGTGGAATTACCAGAAGACAATCAAAATGGGTTGTTATTTCCAAATAGGGAATGAAGTGAATATGAATATGTCTACAGAAGATGCATTCAGAATATCAGTTGAGACAGTTGTTGATTGGATTGCCCGTGAAGTAAACAGGAACAAAACATATGTCCTTTTCCGAACTTATGCTCCTGTGCATTTCAGGGGTGGTGACTGGAATACTGGTGGTGGTTGTCATTCGGAAACACTACCTGATTTAGGCTTCTTACCGACACAGTCCGACATGCATTTCAGTACCGTCGCTAATGTCTTGCCACAACGCGCAAATAAATCAGAAGTGCTGAATTTGGACTTACTTAATGTCACACAAATGTCAGCACGCAGGAAAGATGGCCACGCATCTATTTACTATATCGGGCCACAGAGAACAGCCTCTATGCAGCGACAAGACTGTAGTCATTGGTGCCTCCCCGGGGTTCCCGATTCATGGAACGAGATTCTTTATTCTCTATTTCTCAAGAAGACTTTTTCAGGTCACAATTCAACTGAAGTTTCTCAAGTTTCACTATAAATAGCTTATACAGTTTGCGTGGAATCGCACATATACAAATCTTCGTCAACTCTACTTAATCATTGGCGAATGGCATGAAGCTGGAAGCTAAATTTGCCATCTTAATTTTTGTGGCCAGTTTCACTAATTGGTGCAACTAACTATCTGTTAGTTTGCCTTGTGTAAAGCAAATAAATTAACATGTTTGGTTTAAGAAAAATGTTTCCTGTTTCTATTTTCTATTTTACTAAGAGTTACAAACACATCCTGTAATTTCACTTTATTTCCTATCTTTGAAGATTTGTAGAGTATAAACTATACAAAATAGTTTTCTTTTACAAATCCTTGAAAATTTGAAATAAAGTGAAAATAAGATATTGTGTTCATAACTATCAGTTAAAATTGAAAATACAAACAGAGAAAAAGTTTTCTTAAATTGAACATGCTAAGTTATTATTTAGATGAATGTGGAATGGTGAATTTGAGGCATAGCAGATAGCTTATAGAAAAATAACAATCTTAGTATCAGAAAGCATACTGCATTTTTTTGGACGGTAAATCTCAAGTTCATTGGTCTTGAAAATATTAGNANAGATGTGCCAAGTTTTTTTGAC
TARGET=305,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2519
PRIMER_LEFT_PENALTY=0.134571
PRIMER_RIGHT_PENALTY=0.117281
PRIMER_LEFT_SEQUENCE=TTCGGTTTCTTTTTCCTCTCTG
PRIMER_RIGHT_SEQUENCE=TCTACCATTTTCAGAGCAACGA
PRIMER_LEFT=163,22
PRIMER_RIGHT=453,22
PRIMER_LEFT_TM=59.865
PRIMER_RIGHT_TM=59.883
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.2930
PRIMER_LEFT_1_PENALTY=0.258908
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=CCATGGAACCAAACTCTTTACC
PRIMER_RIGHT_1_SEQUENCE=CAATCTTTAGGTTGCCATCTCC
PRIMER_LEFT_1=237,22
PRIMER_RIGHT_1=497,22
PRIMER_LEFT_1_TM=59.741
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.2930
PRIMER_LEFT_2_PENALTY=0.258908
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=CCCATGGAACCAAACTCTTTAC
PRIMER_RIGHT_2_SEQUENCE=CAATCTTTAGGTTGCCATCTCC
PRIMER_LEFT_2=236,22
PRIMER_RIGHT_2=497,22
PRIMER_LEFT_2_TM=59.741
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=262
PRIMER_PAIR_PENALTY_3=0.2930
PRIMER_LEFT_3_PENALTY=0.258908
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=CCATGGAACCAAACTCTTTACC
PRIMER_RIGHT_3_SEQUENCE=AATCTTTAGGTTGCCATCTCCA
PRIMER_LEFT_3=237,22
PRIMER_RIGHT_3=496,22
PRIMER_LEFT_3_TM=59.741
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.2930
PRIMER_LEFT_4_PENALTY=0.258908
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=CCCATGGAACCAAACTCTTTAC
PRIMER_RIGHT_4_SEQUENCE=AATCTTTAGGTTGCCATCTCCA
PRIMER_LEFT_4=236,22
PRIMER_RIGHT_4=496,22
PRIMER_LEFT_4_TM=59.741
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=261
=
PRIMER_SEQUENCE_ID=SSR_05733 Ps_contig_mira-and-tgicl-ass_franssen_39676
SEQUENCE=AGTTTTTTAAACTTNAANTANTAATANANTAAATTAAYTACAGAAAAATTAATGTCAATAGTAATATTATAATATAACAACAAAGGTAGGTGACAACAATAACCAATTAGACCTCATCCATAGAACTAACACCTAATCATAACCCTTTGCACTTTATGATACACACATTCACTTATTCCTTATCACCATTTGGTACTAGCTAGTGCTTACAATATCTCATCAACAAACTCAATACAAAACAAATCAACAATTCTCTCACAGTTTTCAATCTCGACCGTCCGATCAAAATTAATACCAAAAATATTTTAAAACCAATTTTCATTTGATCTCAAATTAACGGTCGAGATTGTCTATAGAATTTAAATTTAAATGCGTGTTTTTTCACCGCAGAGAATCCAAATCCGAGTCAGTCTCAATATTGTAATATGTCTGATGAAGCCATAATAGGCTGATTCATATAAGACATATTACTCAGAGGGTTGAAGAAGAAGAAGCTATTGTTTGGCTCAACAAAAACTCCATTGCTTGAATTCAATAACCCCAACAATCTCATTCAAAGATTCTAACCTGTTGCTCAATTCACCAATCTGACG
TARGET=482,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05734 Ps_contig_mira-and-tgicl-ass_franssen_39683
SEQUENCE=TCGGGGTTTATTGTAATTCGAACCAGAGTAGTATGCTGATACTACAAGAAACATGCATAGACGAGGCTGGGTCGCTTGTAGTCTACGCGCCGGTGGATATTCCAGCCATGCATGTTGTAATGAACGGTGGTGATTCAGCTTACGTGGCTTTGTTACCTTCCGGTTTCGCTGTTGTCCCCGACGGGCCTGGATCTCGTGGGCCGGAGAACGGGTCTACCGCGAACGGTGGCGAGTCTCGTGTTAGCGGGTCTCTTTTAACAGTGGCGTTTCAGATCCTTGTGAATAGTCTTCCGACGGCGAAACTCACCGTGGAATCAGTTGAAACAGTGAACAATCTCATTTCTTGCACCGTTCAGAAAATCAAAGGTGCACTTCAATGTGAAAGCTGAGTCACGTGAAGATCACGTGATCTGTTGTTTATTTTTCTTTAAATTTATTTTGTTGTTGTTTTGGGTGGTAAATGAATATGTGAAAGATGTAAGTTGGGGTTGGTGGGGGGTTGTTTGAGTCAAGAACGAACCACTCAGTAGGAAAAGAAAAAAAGTTCCTTGCAGTGGTAGGTAAGTTATACGTAAACCGGGTCGGGTCGGGTCGTAGTA
TARGET=578,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05735 Ps_contig_mira-and-tgicl-ass_franssen_39688
SEQUENCE=CCGTCCACCTCCCCCGTCGCATGCAGTTTCCTTTCCTTTCCTCAGTTCCAAGACTCCCTTTAAGTACATTTGCTTGATATCACCCGACATGTTTATGTTATAGGTTGGATCTGAACTCATTTTCTGTAGTTCTTCATCATCAATTTTCTAAATTTTGTTTAACATGATCAAAATCCAGAAGAACCTTCTGGTCAAGTGCATTAGGATTCTGAATAGTTATAAGGTCATCCCTAGTAAATGTCTCATCGGTGAGGAGCTCCTTCCAGTTTTTTGTTTTGATGTTTAACTCCTTAACAGCCTCATAGCAGAAGACATTTCCAGTAGTCTTGACTGCAACAATGTGTGTAAACTCAGTGGTAAAACTTTGTTTAGCACTGGACAGTGATATTCTCCTTCTGAGTTCTTGTGAAAAGTTAGTGGAATGNAGATCTTGCTGCTTCAATGAAGCCCCAGTAACAGGATGTTTACCGTATTTTACAATATATGGAGTTATGTGCATAACATCGAAAACGCTTC
TARGET=27,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05736 Ps_contig_mira-and-tgicl-ass_franssen_39692
SEQUENCE=ATCTTCTTTCTTCAGCATTTGCATCTGTAGAAGCTGAAGCTGCTGTTGGAAATGCTGTCTACATACTTCCTTCCTTCTATAATCATGATTGTGATCCTAATGCACACATTATATGGATAGATAATGCAAAGGCAAAATTGAAGGCCCTCCGGGACATTGATGAAGGTGAAGAACTGAGAATCTGCTATATTGATGCCAGTATGGACCGCGATGCTCGGCGAGAATTATTGTTTCAAGGTTTTGGTTTCCAATGTAATTGTAGTAGATGCTTGCATGGTGATTAGTTTTTTTCTCTAGTAGTTTCTCCTAGGCTTCCAATATATTAAGTGGTTGGAAACAGGCTATGATGTAAAACTGCTCAAATTTTGGGTATTTAGAGTTTTAGATAAGTAGATTCAGTAGAAAAACC
TARGET=66,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0762
PRIMER_LEFT_PENALTY=0.046061
PRIMER_RIGHT_PENALTY=0.030114
PRIMER_LEFT_SEQUENCE=GCATTTGCATCTGTAGAAGCTG
PRIMER_RIGHT_SEQUENCE=CCTTGAAACAATAATTCTCGCC
PRIMER_LEFT=14,22
PRIMER_RIGHT=237,22
PRIMER_LEFT_TM=60.046
PRIMER_RIGHT_TM=59.970
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.3430
PRIMER_LEFT_1_PENALTY=0.312895
PRIMER_RIGHT_1_PENALTY=0.030114
PRIMER_LEFT_1_SEQUENCE=TGCTGTTGGAAATGCTGTCTAC
PRIMER_RIGHT_1_SEQUENCE=CCTTGAAACAATAATTCTCGCC
PRIMER_LEFT_1=40,22
PRIMER_RIGHT_1=237,22
PRIMER_LEFT_1_TM=60.313
PRIMER_RIGHT_1_TM=59.970
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.4706
PRIMER_LEFT_2_PENALTY=0.440473
PRIMER_RIGHT_2_PENALTY=0.030114
PRIMER_LEFT_2_SEQUENCE=CTGCTGTTGGAAATGCTGTCTA
PRIMER_RIGHT_2_SEQUENCE=CCTTGAAACAATAATTCTCGCC
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=237,22
PRIMER_LEFT_2_TM=60.440
PRIMER_RIGHT_2_TM=59.970
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=0.5598
PRIMER_LEFT_3_PENALTY=0.529702
PRIMER_RIGHT_3_PENALTY=0.030114
PRIMER_LEFT_3_SEQUENCE=GTAGAAGCTGAAGCTGCTGTTG
PRIMER_RIGHT_3_SEQUENCE=CCTTGAAACAATAATTCTCGCC
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=237,22
PRIMER_LEFT_3_TM=59.470
PRIMER_RIGHT_3_TM=59.970
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.9845
PRIMER_LEFT_4_PENALTY=0.046061
PRIMER_RIGHT_4_PENALTY=0.938404
PRIMER_LEFT_4_SEQUENCE=GCATTTGCATCTGTAGAAGCTG
PRIMER_RIGHT_4_SEQUENCE=CTTGAAACAATAATTCTCGCCG
PRIMER_LEFT_4=14,22
PRIMER_RIGHT_4=236,22
PRIMER_LEFT_4_TM=60.046
PRIMER_RIGHT_4_TM=60.938
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=13.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=223
=
PRIMER_SEQUENCE_ID=SSR_05737 Ps_contig_mira-and-tgicl-ass_franssen_39693
SEQUENCE=GATTTTACTCTTCTTGACAATACATGGATCGCAACGCATGTGAAAGTGATTGAGAATCCAAATCCAAATCCAAAGCCAAAGCTTACCGTTGAATTTTCATTCATTCACTCATTCTCCACTTTCACTCCACTTCAACTCCCAACCTTCAAACACAAGACACTCAACAACTTTGCCTCCATCCATTTCCAATTTCATGGGACAAATCTTCGACAAATTTCAAGGTGAAGCGTGGAGAAAAAAGCAAATTAGGAAGATTACTGATCGAGTTTTTCAGAAAATAAGGAATCAAATTCAAACGGAAAATTTGAGTTTTGAAGATCTGTATATTGCTGTTTTACTTGTTTACAATGATATCAACAAGTTTATTCCTGGTCCCCATTTCGATCCTCCATCAAAAGAAAACGTCAGACAAATCATGCAGGAATGTGATATGAACTTGGATGGGACAATCGATCACGACGAATTTTGCGATTTCATCCAGAAAATGACAGCTGATACATTCACAGTTGTTAGTCAAAAACTTATTTTGACATTGGTTGTAGCACCTACTGTCGCAATGGCAACAAAAAGAGCCACTGAAGGTGTCCCCGGTGTTGGAAAAGTGGTGCAAAGGTTACCAAATGCACTTTATGCATCCCTTGTTACCCTTGCAGTTGTGTGGTTCCAAAAGATGGGAGATCAGCCTGCAATCTGATTAGTACTATCATTTTTTATAAAGAAAACCATAGAAATTATCATGATTGTACATATCATTACCACTGTTTGCATGTTATGAAATTGCTGTCCTGAATAAGAATGGTTGTATTTGACATTTTGAACGAAAAATATGGTCTTGACTTTTCTGTGANAGGTTATGCACGTGATAGTAAAAGTAGATGAGATAAGCTCTACAAAATTTNTGAATTAAAAATAATGATGTAGAAATGACTTCATTACGAAAAACC
TARGET=96,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1878
PRIMER_LEFT_PENALTY=0.175676
PRIMER_RIGHT_PENALTY=0.012153
PRIMER_LEFT_SEQUENCE=AACGCATGTGAAAGTGATTGAG
PRIMER_RIGHT_SEQUENCE=GTTTGAAGGTTGGGAGTTGAAG
PRIMER_LEFT=32,22
PRIMER_RIGHT=150,22
PRIMER_LEFT_TM=60.176
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.1878
PRIMER_LEFT_1_PENALTY=0.175676
PRIMER_RIGHT_1_PENALTY=0.012153
PRIMER_LEFT_1_SEQUENCE=AACGCATGTGAAAGTGATTGAG
PRIMER_RIGHT_1_SEQUENCE=TTTGAAGGTTGGGAGTTGAAGT
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=149,22
PRIMER_LEFT_1_TM=60.176
PRIMER_RIGHT_1_TM=60.012
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=0.2916
PRIMER_LEFT_2_PENALTY=0.175676
PRIMER_RIGHT_2_PENALTY=0.115965
PRIMER_LEFT_2_SEQUENCE=AACGCATGTGAAAGTGATTGAG
PRIMER_RIGHT_2_SEQUENCE=TGAAGTGGAGTGAAAGTGGAGA
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=133,22
PRIMER_LEFT_2_TM=60.176
PRIMER_RIGHT_2_TM=59.884
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=102
PRIMER_PAIR_PENALTY_3=0.3087
PRIMER_LEFT_3_PENALTY=0.127927
PRIMER_RIGHT_3_PENALTY=0.180765
PRIMER_LEFT_3_SEQUENCE=CAAATCCAAAGCCAAAGCTTAC
PRIMER_RIGHT_3_SEQUENCE=GGCAAAGTTGTTGAGTGTCTTG
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=173,22
PRIMER_LEFT_3_TM=60.128
PRIMER_RIGHT_3_TM=59.819
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=110
PRIMER_PAIR_PENALTY_4=0.3214
PRIMER_LEFT_4_PENALTY=0.175676
PRIMER_RIGHT_4_PENALTY=0.145769
PRIMER_LEFT_4_SEQUENCE=AACGCATGTGAAAGTGATTGAG
PRIMER_RIGHT_4_SEQUENCE=GGGAGTTGAAGTGGAGTGAAAG
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=139,22
PRIMER_LEFT_4_TM=60.176
PRIMER_RIGHT_4_TM=60.146
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_05738 Ps_contig_mira-and-tgicl-ass_franssen_39696
SEQUENCE=CTAAGTATATGACAACTGATATGAGAGCAGCTGTGAGATATGGAACCAGTTTTAGTCAAACAAAAGACTCCGTGTTCTGTTGGAGAGATTTCTTAAAACTCATTTGTCATCCTTTGCCCGATTTTGTTCCTCATTGGCCTGCTTCTCCATCGGATTTTCAAGAAGTGGTTGCCACCTATGCAAAAAGAAAACCAAACAATTGTTCATGGCAATAATGAAAGCTATTCTAGAAAGTTTAGGAATTATGGAAGACAACCAAGAAGAGATGACAAAAGAAAAGGACAATAATGATAATAATAATATTATGAAAGAATTGGACAATGGAAGCCAAATGTTGGTCACCAATTTCTATCCACCATGTCCTGAACCAGACCTAACCTTAGGAATGCACCCTCATTCTGATTATGGCTTCCTCACATTACTTCTTCAAGATGAAGTTGAGGGTTTGCAAATTCAATTTCAAGACAAATGGCTCACGGTTCAACCTATTCCCAACGCTTTCGTCGTCAACATTGGCGATCATTTAGAGATATTTAGCAACGGAAAATAA
TARGET=291,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0233
PRIMER_LEFT_PENALTY=0.009871
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=GCCACCTATGCAAAAAGAAAAC
PRIMER_RIGHT_SEQUENCE=GAATTTGCAAACCCTCAACTTC
PRIMER_LEFT=170,22
PRIMER_RIGHT=454,22
PRIMER_LEFT_TM=60.010
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.0410
PRIMER_LEFT_1_PENALTY=0.009871
PRIMER_RIGHT_1_PENALTY=0.031122
PRIMER_LEFT_1_SEQUENCE=GCCACCTATGCAAAAAGAAAAC
PRIMER_RIGHT_1_SEQUENCE=AGGTTAGGTCTGGTTCAGGACA
PRIMER_LEFT_1=170,22
PRIMER_RIGHT_1=379,22
PRIMER_LEFT_1_TM=60.010
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=210
PRIMER_PAIR_PENALTY_2=0.0584
PRIMER_LEFT_2_PENALTY=0.009871
PRIMER_RIGHT_2_PENALTY=0.048570
PRIMER_LEFT_2_SEQUENCE=GCCACCTATGCAAAAAGAAAAC
PRIMER_RIGHT_2_SEQUENCE=GCTTCCATTGTCCAATTCTTTC
PRIMER_LEFT_2=170,22
PRIMER_RIGHT_2=327,22
PRIMER_LEFT_2_TM=60.010
PRIMER_RIGHT_2_TM=59.951
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=158
PRIMER_PAIR_PENALTY_3=0.0794
PRIMER_LEFT_3_PENALTY=0.009871
PRIMER_RIGHT_3_PENALTY=0.069526
PRIMER_LEFT_3_SEQUENCE=GCCACCTATGCAAAAAGAAAAC
PRIMER_RIGHT_3_SEQUENCE=GAAGCCATAATCAGAATGAGGG
PRIMER_LEFT_3=170,22
PRIMER_RIGHT_3=411,22
PRIMER_LEFT_3_TM=60.010
PRIMER_RIGHT_3_TM=59.930
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=0.0794
PRIMER_LEFT_4_PENALTY=0.009871
PRIMER_RIGHT_4_PENALTY=0.069526
PRIMER_LEFT_4_SEQUENCE=GCCACCTATGCAAAAAGAAAAC
PRIMER_RIGHT_4_SEQUENCE=GGAAGCCATAATCAGAATGAGG
PRIMER_LEFT_4=170,22
PRIMER_RIGHT_4=412,22
PRIMER_LEFT_4_TM=60.010
PRIMER_RIGHT_4_TM=59.930
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_05739 Ps_contig_mira-and-tgicl-ass_franssen_39701
SEQUENCE=CCTATTTTAANTACTACAAAATAGAAAATAGCCATTTCCTAACCAAAGGTNNAATAAAGAAGAGCACAGAAGACCCAAAAACTAAGTCAAATAGTTACACTAGCTGAGCAAGCAAATACACATGCTTAACCACAATTATCACATGACAATTGCAGAATAGACAGTGTTCACTACTAAGAACATAATAAAGAAGCGAAAAACATACAAAAAACTCATTAGGAAATTGGGTGTAGAACAATCTCTGATAAGCTAGAATCAGAAAACTGTTCCTCTATCTGCATTTGATGTGGTTGTTCTCCTTGCCATGTCCAAACAATGTCCTTCAGGTTGTTCTCCCTGCCATGTCCAAACAATGTCCTTAAGTGAAGGCCTAACATTCTCTTTCAAAAGCTTTTGGTACTCCAATGTGTCTCCGTTCGACTTTTTCAACTCGACTAAATGGAAGACCGGTGTGATCTCGAAAATCTCTGCATCGATACCTAAACGTCCTTTCCTTCCTTCCTTCAAACCTTCTAACTTGAGCAAACCTCCGTCTTTTTTCTTCAATTTTAGCCCGAGACTCTTGCAAATTTCTTCAAACTTGGAGATGATAACGGAAACAGGCTTGTTAGACGTAAATCGCATCTCTGTCTTTCGTTCTTTCTCATCAAACAAACTGGATAAGTCGAAACCTGACGAAAAGGAGATGATATCGAAAGCGTTTAAGTTACTGCAAGGTTTCGGAAGATCAACATTATCGCCATTCTCAGAAGCTTTAAATACTTCATCTATGTCGAGAGGAGCTAGTTCTTTGTTGTCTGTGTCGGTAACAATAGGCTTCTTTAACCCCTTTTTGAACCAAGAACTTTCCATGATTTTGGCCAAAGATATCCTCGACTTTGGATTTGGATCCAATATTTTCGACAACAATCTTCGTACGTCTGATGAAAACCAGTTAGGATATTTGAAATACCGCCTTACCAATTTTTTATTACATCTCTATCAAATTTGGATCTCTAAACGGAAGAAAACCAGCCAACATAACATACAAGATCACACCACATGACCATATATCAGTCTTTANGTCCCATTCCGTTACTTTCCCGTTTTTCGGGTTNAACTTCCGTACCGGTTTCCGGGG
TARGET=491,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1160
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.107763
PRIMER_LEFT_SEQUENCE=TTTGATGTGGTTGTTCTCCTTG
PRIMER_RIGHT_SEQUENCE=AAGAAATTTGCAAGAGTCTCGG
PRIMER_LEFT=280,22
PRIMER_RIGHT=574,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=0.1416
PRIMER_LEFT_1_PENALTY=0.039879
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=ATGTGTCTCCGTTCGACTTTTT
PRIMER_RIGHT_1_SEQUENCE=ACAAGCCTGTTTCCGTTATCAT
PRIMER_LEFT_1=404,22
PRIMER_RIGHT_1=607,22
PRIMER_LEFT_1_TM=60.040
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.1416
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.101680
PRIMER_LEFT_2_SEQUENCE=AATGTGTCTCCGTTCGACTTTT
PRIMER_RIGHT_2_SEQUENCE=ACAAGCCTGTTTCCGTTATCAT
PRIMER_LEFT_2=403,22
PRIMER_RIGHT_2=607,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.1451
PRIMER_LEFT_3_PENALTY=0.037339
PRIMER_RIGHT_3_PENALTY=0.107763
PRIMER_LEFT_3_SEQUENCE=CTGCATTTGATGTGGTTGTTCT
PRIMER_RIGHT_3_SEQUENCE=AAGAAATTTGCAAGAGTCTCGG
PRIMER_LEFT_3=275,22
PRIMER_RIGHT_3=574,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=59.892
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=300
PRIMER_PAIR_PENALTY_4=0.1476
PRIMER_LEFT_4_PENALTY=0.039879
PRIMER_RIGHT_4_PENALTY=0.107763
PRIMER_LEFT_4_SEQUENCE=ATGTGTCTCCGTTCGACTTTTT
PRIMER_RIGHT_4_SEQUENCE=AAGAAATTTGCAAGAGTCTCGG
PRIMER_LEFT_4=404,22
PRIMER_RIGHT_4=574,22
PRIMER_LEFT_4_TM=60.040
PRIMER_RIGHT_4_TM=59.892
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=171
=
PRIMER_SEQUENCE_ID=SSR_05740 Ps_contig_mira-and-tgicl-ass_franssen_39718
SEQUENCE=CCGTTTCGTCCCGTATTCCCCCTCCGTTTGGTCCGGTCGGTTCGTTCCTACGAAAAGGACCAATGGTAGTTCTAACGACAGAGTAACGCGGGAAAAGTAAAGNARAAAAACCARAAATCCGAAAAAAAAGTAAGAAAAGTGCTTTCACTCACTCTCTCACTCACCCTGAAGCGATTCCTCCGTAATCTTCTCCCAGGTTCGGTGTTTTCGCCATTCTCTTCGCACATTTTCTACGGATCTAGTTTCTCACCATGAATCGCCACCACGATCCCAATCCATTCGAAGAAGAAGAAGAAGTCAATCCGTTTTCGAATGGTGCTGGATCAAAATCACGTGTTCCACCGTCATCTGAACCACTAGGCTTTGGTCAAAGACATGATGCTACGGTTGATATTCCTTTGGAAACTTCAAATGGGGACTCCAAGAAAAGAAGTCAAGAGCTAGCAGCTTGGGAAGCTGANCTAAA
TARGET=282,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05741 Ps_contig_mira-and-tgicl-ass_franssen_39720
SEQUENCE=CGGGGATTGAAGCATGTGTGTATATAACAAGAAGAATAATAACTCCAGCTATCTTCCATTTTGTAATCTTCACTCCTCTCTCTAACTCTAACCTCCTTCTCCATTTTTCACCACTCTCATCTAAAACCTTCAGATCAATATCTCATTTTCTTAAATGGCTTCTGTTTCATATGCACACACTTGATTCAAAATACTACAAAATTTCTACTTAGTACTTTTTTTCAAAGAAGTTCTTTGACGATGGGTAGAGGCCGAGGAAAAGGTAAGAAGCAATCTGCTATTATTGAGGATCTTGTAAGCGACGAAGAGGATAAAGTACCAGCTCACAGGAAAAGAGGAAGACCGTTAAAACCGGTCACGGATGAAAACGAAGACGTCGTTGATGTAACTGAGAAGATCGAAAAAGGTGAGGAGAATGTAAATGGCAATGTTTCCAACAATGATTTGAAAACCGAGGTTATCACCGTGAATAAAAGGAAAAGGAAGAGGTCTTCCCAGGTGAAAGAAAAGATAGAACCAATGAAAGAGGAGAATGGCATCCGTGCAAAGTCTAGCCCTACTGATTCAGTGAAGTCTACTGGATTCCGACAAAATGGAAGCAGGCGTAAAAGCAAGCCTCGCCGCGCCGCTGAAGCTGGCGTAGATTGCAAGTGAGCAAGGTATTTTGGTAATAGTTTCATGACCTTGGAACCTTGGTATCCCAAGTCAGAGTTTGTTTGATTATATAAGGCTTCTTATGGCGGCTGCTGCTGCTGCTTATTATTATTGTAACTTTCATGTGCTTTATTTTAGTTTTGAGGCTTCATAGTAGACTATCCTTCTATAGAGATACTATTGCTAGAAAAAAAAACTATTGAAATTTTGTAGATATTCTTTTATCATGATGAGTTTGATTTCCTCTTGCTTTNACAAACT
TARGET=743,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.8248
PRIMER_LEFT_PENALTY=0.753123
PRIMER_RIGHT_PENALTY=2.071693
PRIMER_LEFT_SEQUENCE=GTTTCATGACCTTGGAACCTTG
PRIMER_RIGHT_SEQUENCE=GCAAGAGGAAATCAAACTCATC
PRIMER_LEFT=673,22
PRIMER_RIGHT=903,22
PRIMER_LEFT_TM=60.753
PRIMER_RIGHT_TM=57.928
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=3.0603
PRIMER_LEFT_1_PENALTY=0.988635
PRIMER_RIGHT_1_PENALTY=2.071693
PRIMER_LEFT_1_SEQUENCE=AGTTTCATGACCTTGGAACCTT
PRIMER_RIGHT_1_SEQUENCE=GCAAGAGGAAATCAAACTCATC
PRIMER_LEFT_1=672,22
PRIMER_RIGHT_1=903,22
PRIMER_LEFT_1_TM=59.011
PRIMER_RIGHT_1_TM=57.928
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=3.0744
PRIMER_LEFT_2_PENALTY=1.002664
PRIMER_RIGHT_2_PENALTY=2.071693
PRIMER_LEFT_2_SEQUENCE=GCAAGTGAGCAAGGTATTTTGG
PRIMER_RIGHT_2_SEQUENCE=GCAAGAGGAAATCAAACTCATC
PRIMER_LEFT_2=646,22
PRIMER_RIGHT_2=903,22
PRIMER_LEFT_2_TM=61.003
PRIMER_RIGHT_2_TM=57.928
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=3.1156
PRIMER_LEFT_3_PENALTY=1.043868
PRIMER_RIGHT_3_PENALTY=2.071693
PRIMER_LEFT_3_SEQUENCE=TTTCATGACCTTGGAACCTTG
PRIMER_RIGHT_3_SEQUENCE=GCAAGAGGAAATCAAACTCATC
PRIMER_LEFT_3=674,21
PRIMER_RIGHT_3=903,22
PRIMER_LEFT_3_TM=59.956
PRIMER_RIGHT_3_TM=57.928
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=230
PRIMER_PAIR_PENALTY_4=3.2335
PRIMER_LEFT_4_PENALTY=1.161853
PRIMER_RIGHT_4_PENALTY=2.071693
PRIMER_LEFT_4_SEQUENCE=CATGACCTTGGAACCTTGGTA
PRIMER_RIGHT_4_SEQUENCE=GCAAGAGGAAATCAAACTCATC
PRIMER_LEFT_4=677,21
PRIMER_RIGHT_4=903,22
PRIMER_LEFT_4_TM=59.838
PRIMER_RIGHT_4_TM=57.928
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_05742 Ps_contig_mira-and-tgicl-ass_franssen_39723
SEQUENCE=CATACTGCTCATATCCAATGCCAATGGTTCGTTACCATTAGGTGCATTGAAATAGGCCTGAAATTTTCAAATTAGATTAGAAGCTTTTGATTATAGACTTCAATATATGTTATAGTAATGTAAACCAGTAACTAACTAACTGTTCTTACCTTGTGCCATTTCAACTGTGGCTGGTTTTGAGAAGCTAATGACTCTCTCACCCAATCAACAGATGATACACCGTTGGGAGATACCAGATTCATCGCTTCGCCTTTTAGGCCGACCTGGTATGACCATTTCTGCCATGTCAAATCTTTTTGTCCGTGATCGAGACCTTTGAGCAAAACTGGACCGGTGATTCCTGTCTTCCATGATTCAAAATGGATTCCA
TARGET=129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2215
PRIMER_LEFT_PENALTY=0.110393
PRIMER_RIGHT_PENALTY=0.111077
PRIMER_LEFT_SEQUENCE=CGTTACCATTAGGTGCATTGAA
PRIMER_RIGHT_SEQUENCE=CATCTGTTGATTGGGTGAGAGA
PRIMER_LEFT=29,22
PRIMER_RIGHT=213,22
PRIMER_LEFT_TM=59.890
PRIMER_RIGHT_TM=60.111
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=0.2215
PRIMER_LEFT_1_PENALTY=0.110393
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=TTCGTTACCATTAGGTGCATTG
PRIMER_RIGHT_1_SEQUENCE=CATCTGTTGATTGGGTGAGAGA
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=213,22
PRIMER_LEFT_1_TM=59.890
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.2215
PRIMER_LEFT_2_PENALTY=0.110393
PRIMER_RIGHT_2_PENALTY=0.111077
PRIMER_LEFT_2_SEQUENCE=CGTTACCATTAGGTGCATTGAA
PRIMER_RIGHT_2_SEQUENCE=TCATCTGTTGATTGGGTGAGAG
PRIMER_LEFT_2=29,22
PRIMER_RIGHT_2=214,22
PRIMER_LEFT_2_TM=59.890
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=0.2215
PRIMER_LEFT_3_PENALTY=0.110393
PRIMER_RIGHT_3_PENALTY=0.111077
PRIMER_LEFT_3_SEQUENCE=TTCGTTACCATTAGGTGCATTG
PRIMER_RIGHT_3_SEQUENCE=TCATCTGTTGATTGGGTGAGAG
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=214,22
PRIMER_LEFT_3_TM=59.890
PRIMER_RIGHT_3_TM=60.111
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=188
PRIMER_PAIR_PENALTY_4=0.2408
PRIMER_LEFT_4_PENALTY=0.110393
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=TTCGTTACCATTAGGTGCATTG
PRIMER_RIGHT_4_SEQUENCE=CTGGTATCTCCCAACGGTGTAT
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.890
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_05743 Ps_contig_mira-and-tgicl-ass_franssen_39724
SEQUENCE=TTCCCTTACCCTACGAATAGTTTCTTGTNNGAGAAATTACTTAAGATAAAATTAGAAAGTATAGTTAGAAGTTTGCATCCAACTCATCAGTTTTAAAGTAAGGAGACCAATCTAGACTTTCGGTTTGCGAAATCATCTGAACTGTATCCCTCAAAAGATGTCTCAAGCAATTATCAACGACAACGAAGTTGACATTGTGATTGGTGCACTAAACTCTGACCTAACAACTTTCATGAATGAATGGAAGCCAGTTTTCTCTCGTTTCCACCTGATAATAGTCAAAGACCCTGACCTCAATGGAGAACTCCGAATTCCTGATGGATTTAGTGTAGATGTCTATACAAAATCTGAAATTGAGCGAGTGGTGGGTTCCTCCACTTCAATTCGCTTCTCTGGCTACGCTTGTAGATATTTTGGCTTTCTAGTTTCAAAGAAGAAGTATGTTGTCTGTATTGATGATGATTGTGTTCCAGCAAAAGATGATGCGGGGAATTTAGTAGATGCTGTGGCTCAGCATATTGTGAACCTCAAGACTCCTGCCACTCCTTTCTTCTTCAATACACTATATGATCCATTCCGTAAGGGTGCAGATTTTGTTCGTGGTTATCCATTTAGTCTGCGAAGTGGGGTTGATTGTGCTCTGTCTTGTGGACTATGGCTCAATCTGGCGGACCTTGATGCACCAACACAAGCTCTCAAGCCAACACAGAGGAATTCACGTTATGTGGATGCAGTTCTGACTGTTCCTGTGAGAGCCATGTTGCCAGTGAGTGGAATCAACATCGCCTTTAACCGAGAAGCAATTGGTCCGGCATTAGTCCCGGCTTTGGTTTTGGCAGGAGAAGGAAAACTGAGGTGGGAAACTGTGGAAGATATTTGGTGTGGATTGTGTGTGAAAGCTATATGCGACCACCTATCCCTTGGTGTGAAAAGTGGGTTGCCATATGTTTGGAGAACTGAAAGAGGCAATGCCATAGACAGCTTGAAGAAAGAATGGGAAGGGGTGAAATTGATGGAGGATGTTGTTCCTTTCTTCCAGTCTGTAAAGTTGCCACAATCAGCTGTCACTGCAGAGGACTGCGTGGTTGAGATGGCCAAATCAGTGAAGGAACAACTTGGGAAGGCGGATCCTATGTTTTCGCACGCCGCTGATGCTATGGAAGAGTGGGTCAAGCTCTGGAAGTCAGTTGGATCTGCTTGAAGTTTCTGAACTAGTGGTTGACATTGTCTGGATTTGTTTCATCCTCGCATTAGGATTTGTAGGAGTTATAAGGTTTTTGCCTTAATGGCTTCTATATTTCTTTTCTTTTCTTTTATTTAGATTTAAGCTACGTTATATCTGCTTTCCTTTTTTTTAAGTCAACTAAGTTTACTTTTACCTGACTGAGTTAGTTTCAAGGAAAAAATAAAATAGTACTGGTATGGTTTTGTGCCAATTGAATAAAATAGCACTGGTCTGGTTTGTGACAATTGAATAAAGTTGTCTCGTGGCTTTAAATAG
TARGET=1298,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0767
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=0.069737
PRIMER_LEFT_SEQUENCE=TGGATCTGCTTGAAGTTTCTGA
PRIMER_RIGHT_SEQUENCE=ATTGTCACAAACCAGACCAGTG
PRIMER_LEFT=1188,22
PRIMER_RIGHT=1470,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=59.930
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.1316
PRIMER_LEFT_1_PENALTY=0.006945
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=TGGATCTGCTTGAAGTTTCTGA
PRIMER_RIGHT_1_SEQUENCE=TTCAATTGTCACAAACCAGACC
PRIMER_LEFT_1=1188,22
PRIMER_RIGHT_1=1474,22
PRIMER_LEFT_1_TM=59.993
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.1849
PRIMER_LEFT_2_PENALTY=0.115189
PRIMER_RIGHT_2_PENALTY=0.069737
PRIMER_LEFT_2_SEQUENCE=GCTCTGGAAGTCAGTTGGATCT
PRIMER_RIGHT_2_SEQUENCE=ATTGTCACAAACCAGACCAGTG
PRIMER_LEFT_2=1173,22
PRIMER_RIGHT_2=1470,22
PRIMER_LEFT_2_TM=59.885
PRIMER_RIGHT_2_TM=59.930
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=298
PRIMER_PAIR_PENALTY_3=0.2222
PRIMER_LEFT_3_PENALTY=0.006945
PRIMER_RIGHT_3_PENALTY=0.215254
PRIMER_LEFT_3_SEQUENCE=TGGATCTGCTTGAAGTTTCTGA
PRIMER_RIGHT_3_SEQUENCE=GTCACAAACCAGACCAGTGCTA
PRIMER_LEFT_3=1188,22
PRIMER_RIGHT_3=1467,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=60.215
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.3304
PRIMER_LEFT_4_PENALTY=0.115189
PRIMER_RIGHT_4_PENALTY=0.215254
PRIMER_LEFT_4_SEQUENCE=GCTCTGGAAGTCAGTTGGATCT
PRIMER_RIGHT_4_SEQUENCE=GTCACAAACCAGACCAGTGCTA
PRIMER_LEFT_4=1173,22
PRIMER_RIGHT_4=1467,22
PRIMER_LEFT_4_TM=59.885
PRIMER_RIGHT_4_TM=60.215
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_05744 Ps_contig_mira-and-tgicl-ass_franssen_39728
SEQUENCE=ATACNGGTTCAAAATTTCATATTTTAAATATTGGAGTGTAGTAAATGGAAAGAGTTCAATCCCATACACAATATAAAGTTATACAAAATGTTAACAATAAAAATACAATTAGGTAAGTATAACTATATATGTAATCTATTCAAATATGTGTATGTGTGTGTGAAAAATTATTAGAACTTTGACTGCAAATACTGTATGAACATAGTGTCGACCATCAAGCCATGTTAGAGACCCAAAATTGTATCCACCATGAGCTTCCTTGGCATAGAATGACACTTTATAGGTAATTCTTTTAACATTCTTTGAAAACACAAGTTTGTTGGGGTAGACATTAACGACCATTCCTTCAGGTGCATGCACCTTTGCAACGTATGTGGCATTAGAGGCTCCCACATTGGTGACTGTTCTTGTGATCATTTTTACTTTTTGATTTCTTTTAAGTGTTTGTATAGAGATTGATGGGTAATTGATGTTGGATATAAGATCTTGAGAGGTTTTTGGGCAAGTTACATTGGTTTTAGATACTGAGCTTATGATTTTCTTTGAGTAGCCATAATAACAAAGGAATTTGATGTAGTCTTTGATATTTGTTTCGAAGACTAAACCAGGGTTGAGAGCCTTGAGTGGATTGATTTCTCCAACTCCCATTTCGTGTGGGTTGGCGATGTAGTTTGAGCTGTTGGTCACCGGCTTCTTCATGCTATTGTAGGTTGTAGCTGTTGTCATTAATGCTGATTTGATCATTGAAGGACTCCATCTTCCATGTACTGATTTGATGAATGCTGCAGCACCTGTCACATGAGGGCAAGCCATAGATGTACCGGATTTTATGCCAAATAAGGATGGCTTTTCTCCAATTGGAACACTTCCTGGCTCATCACTTTTGGGAACCATGGCACCCAAAATGGCAACTCCTGGAGCCATGACATCAGGCTTGAGAATGTTCTCTGTAAGGCTAGAAGGACCTCTGGATAGAAAAGG
TARGET=153,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9550
PRIMER_LEFT_PENALTY=1.954288
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=GGAAAGAGTTCAATCCCATACA
PRIMER_RIGHT_SEQUENCE=TTTTGGGTCTCTAACATGGCTT
PRIMER_LEFT=46,22
PRIMER_RIGHT=237,22
PRIMER_LEFT_TM=58.046
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=192
PRIMER_PAIR_PENALTY_1=1.9550
PRIMER_LEFT_1_PENALTY=1.954288
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=TGGAAAGAGTTCAATCCCATAC
PRIMER_RIGHT_1_SEQUENCE=TTTTGGGTCTCTAACATGGCTT
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=237,22
PRIMER_LEFT_1_TM=58.046
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=193
PRIMER_PAIR_PENALTY_2=2.1175
PRIMER_LEFT_2_PENALTY=2.116797
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=GGAAAGAGTTCAATCCCATACAC
PRIMER_RIGHT_2_SEQUENCE=TTTTGGGTCTCTAACATGGCTT
PRIMER_LEFT_2=46,23
PRIMER_RIGHT_2=237,22
PRIMER_LEFT_2_TM=58.883
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=192
PRIMER_PAIR_PENALTY_3=2.1188
PRIMER_LEFT_3_PENALTY=1.954288
PRIMER_RIGHT_3_PENALTY=0.164560
PRIMER_LEFT_3_SEQUENCE=GGAAAGAGTTCAATCCCATACA
PRIMER_RIGHT_3_SEQUENCE=ACCCCAACAAACTTGTGTTTTC
PRIMER_LEFT_3=46,22
PRIMER_RIGHT_3=325,22
PRIMER_LEFT_3_TM=58.046
PRIMER_RIGHT_3_TM=60.165
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=2.1188
PRIMER_LEFT_4_PENALTY=1.954288
PRIMER_RIGHT_4_PENALTY=0.164560
PRIMER_LEFT_4_SEQUENCE=TGGAAAGAGTTCAATCCCATAC
PRIMER_RIGHT_4_SEQUENCE=ACCCCAACAAACTTGTGTTTTC
PRIMER_LEFT_4=45,22
PRIMER_RIGHT_4=325,22
PRIMER_LEFT_4_TM=58.046
PRIMER_RIGHT_4_TM=60.165
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_05745 Ps_contig_mira-and-tgicl-ass_franssen_39743
SEQUENCE=GTCGGGACGGTTACAAAATTTCCTCTTTAGTTTAGTTGAGTTGAAACACTAATTACTTCACCCATTGTGAGCAATTGACAATTCCTAACCGAAAGTTCTTGAGGGTTTATTGCCACATTTCAATCGATCAGAGAACAATGGTCAATCGCCGATTCACTCAGGTAGCCACCAGCGACGAAGATGAAGACGAAGCACCACCGCCACCTCAGCAACATTCCAAGCTACGCAAACGGAAGAGAATGAAGCTTCTAGACGAAGAAGAAGAAGACAACGACAATAGCGATAACGATGAGGAGAAGGAAGAGGAGAAAAAGGATCCGCCTCAGACTCCAGATGATGCTAAACCAATTGGTGATCCTCTTAGGGTTTCGGGGAAAGGAAGAGGGAGAAAGAGACATTACGAATCCTTCGAATTCGATGGCAACCAATACTATCTCGAGGATCCTGTTCTTCTTGTGCCTGAGGATAAAGAACAAAAGCCCTATGTTGCTATAGTTAAAGACATTATACAGTACTTTAGTGGAAGTATAATGGTGGCAGGACAGTGGTTTTATCGTCCTGAAGAAGCTGAAAAAAAAGGCGGTGGCAGCTGGAAATCGTGTGATACAAGGGAAGCTTTTTTATAGTTTCCACCGGGATGAGGTTCCTGCAGAGTCTGTTATGCACAAGTGTGTGGTGCACTTTGTTCCCTTAAACAAACAGTTTCCCAAACGTAAGCAACATCCTGGCTTTATTGTACAAAAGGTGTATGACACTTTGGAAAGGAAACTCTGGAAGCTTACTGATAAGGACTTTGAAGATGTTAAACAGCAAGAAATCGACGAGCTTGTTCAGAAAACTCAGAAACGCATCGGCGAGTTACTTGACATTGAGCCTGAAGAATCCCCTCCTGTTGATCAGGAGGAGGTGATGAAAAATAAAAGAAGCTTAAGGAGAAAGAGCATTTCACCCATTGATGTTTCAAGGGAGGAGGAAGGAATTCCTAAGAGTGACCAACATTCGAAGCCCGAAACACCCATGAGTTGTGTAAATAATAACTCAGAGCATCATCGCATATTAGTGAATTTCAATGCACTAACTGGAAACGTTCATCGTGACAAATGGTTGGAGAGGCTGCTTCAGCACATTCAGTACATGTGTAATTATGACGAGGGTGTAGAAAAGGGAAAGGGATCAGGAAATGCCGATTCTGACGAAATCAAAATAAAAAACAATGATAAAACATCAGAAATAGCAAATGACTGTCAGGACAAGGGTCAGAAGRGTTCCGA
TARGET=255,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05746 Ps_contig_mira-and-tgicl-ass_franssen_39746
SEQUENCE=TGTGTATATATCCATTCCAAACCTTGTGCATTATGCATGATTGTGTTTGTTTCGAAGATCCGACTAGTAAAAAAGGGAACGTAATATATATGATTCATGAACTTACTTTCATGTTTATTTGTTNNGRTATCAACGCAGAAGAAGTCAGAGCAGGAGTATCTCCCGCAGTCCAGTACGAGGCCGTTATAGAGACAATGGTCGTGGACGGAGTCCAAAACGCAGTCTTAGCCCGGAAGACAGGCGACCTCCCATAAGTGACAGATTAAAATCTCGACTTGGTGCACGAGGTAATCAACAATCTCCAGACAGACAGAAGTCAAAGTCAAATTCGAGGAGCAATGGCATTTCTCGATCCAGATCCCCTGACATTACACCCCCAAAGCGTTATAATAAAAAGACTTCCATATCTCGTAGCAGGTCTAGATCAAGTTCTGCATCAGGACAGAAGGGTTTGGTTTCCTATGGAGATGCTAGTCCTGATTCCGGAGCTAGGTAGGTATATGCTGAATGGATAGCAGCTCTCAAGTCCAAACAGGAACTGAAGTTTCTGAACATTCGGAAGCAAGTTTCAGCAAAATATATCGATAGCAAGTCTCAGCAAAATACCCCGGAGTTACAGGGTGCTGCTCCACCGCCTTTACATATTCTCACGTCTTTAGTTAAGTTCTAATGTTGAGCAGTTAGAAAGTTATTATGCCTTAGACTAAAATCAAGTCCGTTTTGGTTTGGTTTAGGAAAGGTAAAAGGTTTAGTTGGGTACGAACCCTAAAAAACTTGAAGTTA
TARGET=303,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05747 Ps_contig_mira-and-tgicl-ass_franssen_39752
SEQUENCE=TAGTAAAGTAAAGCCAAAAATTGGTCAAAACTCAAAACATAAATTTTATTCCATGAAAAATTCAAAGGCTCATACCAAATACATCAAAAATACTTTATTCTTGCCAGAATTCAAACCTGTTGTCTCTGGTTAGTGCCAACAGAGACAACCTAAAAACAAACACACCCAAGATGAATCATAAAAGTAAATTTACCCAACAATCATATAACATTGTAGCAAATGTTTGATCCCCCAGTCTCAATTTGATTCATTAATGGGAAGAAATTTTAAGGTTGAGGTGGAACCAAGACTTCCAACTACCATTTCCCCTTCGTGAAAGTGACGAAGTCCTACTTCCATTATCAGCTGCTTCATTTGTAGTGTGTGGCTTCTTGCATAGCACGAAATTCCGAGCTCCATGTGATCCGATTTTGTCCCATGGACTCAAAGCCACATCAGATCCATGATGAGGACAATAAAGAAAGAAAGCTCTATGATCACTCCCCAGACGCGGCAATCGCTCTTCGCGTGCATAAGACTTCAGAGTAGTATCAATAACAGCCTCAACAAGCTCCTCTTCATCCACAACAAACCGAATCGGGCCAGCACTTCCCAACACATTGATACTAACCAACAGCCTCTTGCCGCTGTTAGCGTTGTGATTCTTCTTATGCTTGCTCAACAACATCGCGTCCTTATATTACCACACTACAAATCACAAAACCAAAAAACACAAACAACAAACTACGCCCAAGAATCTAGTTACTAAATTGAATCAATTTTGAAAGAATGTGGAAAATTGTTGGATTAGGGTTTCTATGGATCGAGACCGAGGCAGCGATAGTGCCAACCTTGCATGCAAGAAGCGAAGGGGAAGAGAGCGCTCGCCGTTGTTGATGGTAGAAAAGGAGCGCATAGRAATNGNANGGGRAAAGGGGGRAAAGGGGAAAAGGGTAAAACGGGGTTTTCCTAACCTAGTTACCGAACGGTAGATTCGTAGGAAG
TARGET=457,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05748 Ps_contig_mira-and-tgicl-ass_franssen_39758
SEQUENCE=CGGGGGGAGACTCTAATAGCCCTCGATTTTCGTGTCTTCCTCTTTCAAAAAGTCTTCTCCCATTAAAAAGTGCTTTTCCACACCTCTTCTCCCTTCTCATCACCTCTAAACTCTCTCTCTCACACCTCCAATTCTCCACCAAAATTCTCAACCCTAACTTCTTTCCGCCAAATCTTCTACGTCTCACCGTCATGACATCGAATCAAATTTCGCAGTAGAAAAAAAAGCATAAGCATAAAAGAAAAAGCGATTAATTAAGCAAATGGCTTATCATCATGACCACCTATCACATGAAATTAACTTTCAACCATTCACAGAAGAGCAACAGTTAAATCAAAGCAGAGACATGCAAAGGCTACTACCGTTCACCGGCGCCGGAGCTGGATCAGCGCCGAATGGCTCAACAACGCCGTAAACCTACGGTAA
TARGET=111,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0682
PRIMER_LEFT_PENALTY=0.010077
PRIMER_RIGHT_PENALTY=0.058146
PRIMER_LEFT_SEQUENCE=TAATAGCCCTCGATTTTCGTGT
PRIMER_RIGHT_SEQUENCE=ATGTCATGACGGTGAGACGTAG
PRIMER_LEFT=13,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.990
PRIMER_RIGHT_TM=60.058
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=0.1476
PRIMER_LEFT_1_PENALTY=0.010077
PRIMER_RIGHT_1_PENALTY=0.137489
PRIMER_LEFT_1_SEQUENCE=TAATAGCCCTCGATTTTCGTGT
PRIMER_RIGHT_1_SEQUENCE=GGTGAGACGTAGAAGATTTGGC
PRIMER_LEFT_1=13,22
PRIMER_RIGHT_1=187,22
PRIMER_LEFT_1_TM=59.990
PRIMER_RIGHT_1_TM=60.137
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=175
PRIMER_PAIR_PENALTY_2=0.1520
PRIMER_LEFT_2_PENALTY=0.010077
PRIMER_RIGHT_2_PENALTY=0.141882
PRIMER_LEFT_2_SEQUENCE=TAATAGCCCTCGATTTTCGTGT
PRIMER_RIGHT_2_SEQUENCE=TAGAAGATTTGGCGGAAAGAAG
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=178,22
PRIMER_LEFT_2_TM=59.990
PRIMER_RIGHT_2_TM=59.858
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=0.1665
PRIMER_LEFT_3_PENALTY=0.108385
PRIMER_RIGHT_3_PENALTY=0.058146
PRIMER_LEFT_3_SEQUENCE=CTAATAGCCCTCGATTTTCGTG
PRIMER_RIGHT_3_SEQUENCE=ATGTCATGACGGTGAGACGTAG
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=197,22
PRIMER_LEFT_3_TM=60.108
PRIMER_RIGHT_3_TM=60.058
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=0.1888
PRIMER_LEFT_4_PENALTY=0.010077
PRIMER_RIGHT_4_PENALTY=0.178718
PRIMER_LEFT_4_SEQUENCE=TAATAGCCCTCGATTTTCGTGT
PRIMER_RIGHT_4_SEQUENCE=GTCATGACGGTGAGACGTAGAA
PRIMER_LEFT_4=13,22
PRIMER_RIGHT_4=195,22
PRIMER_LEFT_4_TM=59.990
PRIMER_RIGHT_4_TM=60.179
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=183
=
PRIMER_SEQUENCE_ID=SSR_05749 Ps_contig_mira-and-tgicl-ass_franssen_39764
SEQUENCE=AAAGTTAATCACTAGCCTCTTTGGTCCTAAACTGGAACTGTCAAACATGACAAATGAGGAATACAAGAAGGCAGTATTGGAGGCCAAAGAGCACATATTGGCCGGTGATATTTTTCAAATTGTGCTAAGTCAGCGGTTTGAGCGCCGAACTTTTGCAGACCCTTTTGAAGTCTACAGGGCACTGAGAATTGTTAATCCTAGTCCATATATGACTTATTTACAGGCCAGAGGGGATTAGTTTTGGTTGTCTTACAAGTCCAAGTAAATTTCTTACGACGGGTGAAGAAGAAGAAAATCCAACCAAGTCCGGCCTTCGTTCGGCCGGGTA
TARGET=282,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05750 Ps_contig_mira-and-tgicl-ass_franssen_39767
SEQUENCE=CGGGGGGTCCGTTGTAGAATCGGAAAAAAGCTGGTATCCCGCACTGAACTCGAGCAATCGTAAAAAACCAAAAAGCCTGCCGGAAAAACAGCCGGAGTCGTTCGTCGGCCGCCAGAAACAGAAGCAAAAACAACGACAAGAAGCAAAAACAAAAGGATTTGAAATGGAATTGGCAGACAGATCAGTTGGATTTCTATTGTCCTTTATAAGCTTATCGATATTCACTTATTATACTTTCTGGGTTATCATCCTGCCATTTGTAGATGGTGATCATTTCGTACACAAGTATTTCTTACCCCAAGAATATGCCATACTAATACCGGTTTCGGCTTGCGTGGCTCTTCTTTGCCTCTTATGCATATTTATTGGGTTTGTGATGCTCAAATCCAAGAAGAAGAAGGCATAATGTATGTTTTGCACAATTTATTTTTGAAGTTTTGAAACAACAATGTATTTCTAGCATTTTACACCAGAAAGTGAAATAATTGTAACTTTGTATGTCCCTGTGTGAGTGGAGGAGAGACAGACATTTTGTTAATTCCTGTCAATGTCCTGAGTTACTTGCAACATATTATACACACATATATAAATAGAATCCATATGTTTTGAA
TARGET=389,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8605
PRIMER_LEFT_PENALTY=0.366463
PRIMER_RIGHT_PENALTY=0.493998
PRIMER_LEFT_SEQUENCE=TACTAATACCGGTTTCGGCTTG
PRIMER_RIGHT_SEQUENCE=CTGTCTCTCCTCCACTCACACA
PRIMER_LEFT=311,22
PRIMER_RIGHT=525,22
PRIMER_LEFT_TM=60.366
PRIMER_RIGHT_TM=60.494
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=215
PRIMER_PAIR_PENALTY_1=0.8605
PRIMER_LEFT_1_PENALTY=0.366463
PRIMER_RIGHT_1_PENALTY=0.493998
PRIMER_LEFT_1_SEQUENCE=TACTAATACCGGTTTCGGCTTG
PRIMER_RIGHT_1_SEQUENCE=TGTCTCTCCTCCACTCACACAG
PRIMER_LEFT_1=311,22
PRIMER_RIGHT_1=524,22
PRIMER_LEFT_1_TM=60.366
PRIMER_RIGHT_1_TM=60.494
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=0.8675
PRIMER_LEFT_2_PENALTY=0.366463
PRIMER_RIGHT_2_PENALTY=0.501074
PRIMER_LEFT_2_SEQUENCE=TACTAATACCGGTTTCGGCTTG
PRIMER_RIGHT_2_SEQUENCE=CTCCACTCACACAGGGACATAC
PRIMER_LEFT_2=311,22
PRIMER_RIGHT_2=516,22
PRIMER_LEFT_2_TM=60.366
PRIMER_RIGHT_2_TM=59.499
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.8902
PRIMER_LEFT_3_PENALTY=0.366463
PRIMER_RIGHT_3_PENALTY=0.523704
PRIMER_LEFT_3_SEQUENCE=TACTAATACCGGTTTCGGCTTG
PRIMER_RIGHT_3_SEQUENCE=TGTCTGTCTCTCCTCCACTCAC
PRIMER_LEFT_3=311,22
PRIMER_RIGHT_3=528,22
PRIMER_LEFT_3_TM=60.366
PRIMER_RIGHT_3_TM=59.476
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.8902
PRIMER_LEFT_4_PENALTY=0.366463
PRIMER_RIGHT_4_PENALTY=0.523704
PRIMER_LEFT_4_SEQUENCE=TACTAATACCGGTTTCGGCTTG
PRIMER_RIGHT_4_SEQUENCE=TCTGTCTCTCCTCCACTCACAC
PRIMER_LEFT_4=311,22
PRIMER_RIGHT_4=526,22
PRIMER_LEFT_4_TM=60.366
PRIMER_RIGHT_4_TM=59.476
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_05751 Ps_contig_mira-and-tgicl-ass_franssen_39790
SEQUENCE=CCGTCATCATGGCAATTCAATTGCAAATTAACTTCATTTTCATTTTCACAAAGTATTAATTTAAATATTCAGAAAGATAAATTGATCATTTCTCCAGTTTCTTCAACATAAACAACTTAATGCATAACCTGATACTACATCTCCTAAACCATCATCATTGATCTTAGGCATTTTATTATTTTCTCAACTTTTCATAACTAAAACATATTACATCATGAACTTCCAAACACAACACTTAGAGATATCCTTTTTCCTTGCAACACTGAATCGCATCTTCAAACATATCATCAACACTGTACTTAAACTCGAAACCAGCAGCCACGAGTTTCTTCGAGTTCAATTCAGCTAACCTTGCACCTTTAATTTCCTTCAACTCATCTACTGATAGAATTTGAAATTCTGGATATTTGGCTGAAAAAAGTTGAGACATTTCTTCAATAGTTACGATGAATGGTGCACAATTGTATCTCCCTCCAGGAACAGAATTCTCAAGTAGATATATATGTGCTCTAGCTACATCGTCAACATGTACCATATGAAAACGTGTCACACCAATTTGTTCCTTTTTACCTAATACCAAAACAAGAGCTTTGTCAACAGAATCAGGAAGCTTAGGACAAATGAAAGAACCAACAATAAAAGGAAGAATCAAAGTAGCAACATCGATCCCATTTTGTTGGCCGAAATCAAGCACTGCTTTTTCTGCCAAAGTCTTTGAAACTGAGTAAGACCAACCAAATGGTTTAACACTTCTAAGCAAACCAACATCACTCCAATCAGTCTCATCCAACACTTCTTTGTCTTTGCCGTTGAATGAAACAGCTGAGCCACTTGAAGTGTATATGAATCTCTTCAGTGTTTTTGAATTTACGCATGCTTTTAAAATTCCTAATGCTCCGTCCACGGTTCTTTTTGTCACTATTTCTTCTGGTTCGCTCACAGCGAAATCTACTGGTGTTGCCGTGTGGAATACCCCGACGCAGCCTTCGACTGCTGCGGAGAAACTTTCTGGGTCGCTTAGATCCGCGTTGAAGAAATGGAGCCTTTCGGATGCACCGGGAAGGTTTGTGAGGAAGCTAAGATCCCTCTTACGTTCTGGATCAGGTCTAACAGTGGTATTAACAGAGTATCCATTTTCAAGAAGACTCTTAATAATCCATGAACCAAGAAAACCTGTACCTCCTGTTACACAAACCCTTCCTTTCCCCTCTGCCATGTTTGGACACCTCTTTCTGTTAAGTNYNTTCTGTTTGATATATATATATATACTC
TARGET=1254,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05752 Ps_contig_mira-and-tgicl-ass_franssen_39791
SEQUENCE=CTAAGATGCACACAATAAATAACAAAGTGGACGAGAAATAACAGTACTTTGATATACAGACTTTTTATGTTGGCTTGTGGCAAATATTGCTATACAGAAAATAGCTGACTTGGGTTATATATGCAGGTACTATAAAAATTAAAAGTTAAAAAACAACTAATGATGTTACACGATATAAGGTACAAAATTACATCAAACTAATGTCACCCAAGATATATAAAACGAGCAACATATAAATCGCAAATCATGAAGGCACCAAAAACTCTACATCACCATTGTAATAGAAGAATTTCATGTCATTTTATATCCTTTCCAGCTATAGAAGAGATGCCTGTCACTTCAGAACTCGTTGAATCCTTTACATTCTTTCCTGATACGGGACATGAAATGTTTCTTGTTAATTCAGAGCCCAAGCCAAGTTTGGCTTCTGAAGGCAGTGATAGGGATGTTGCAGATGGAGTAATGGAATCTAGGGACATAATAGGCACTTGGGGTAGGTTAAGAGGATTAACTTCAGAGACTTCCTCATTGCTCTCGTCTAAGTAGACAACATCCTGCATTGTAAGCTGATGATATTCAACAACCTCCCTCAGAAAGCGATTCTCACGGGCATAAACCGAGTTCTCATGGGCCAAGCGAGCTTTCTCAGCTAGAAGAGTTTCAAGTTGAAGTCGCACCAAATCATCATCATCTTGGCTATCTCCCCTTTCGCGATTTATACGGAGGATTTTATTTTCTTCCTCTAATTGAGCACATCGATCCTTTGCAAATGCCAGATCAGCTTTGACAGTCTTCAACTCCCGAAGAAGCAATTTTGCTTTGGCAGCCATTGCCATTGCAACGTCGCGAGATGCCTTCAGCTGAAGTTCTTGGTCTGCTTGCTTTGGCGACTTCTGGGTCCGCACCTGGGGTTCTTGCAACGTAGTAGAATGGTTTGTTTCCTGATTTTGTACTAAAATGCTTGGCTTTTTCCTGATATGTTTACGGGTTTCCTGAATGATATCTGAAGTCCGATTCTCAACAATTGTAAAACCTTCCTCGAAAGATTTACCAATGGTGCCACCAATATGAGTAAGCGAGGATGTGATCGCACCTAACCCCCTAATAATTGTTGGACTGTCCCTTTTGAGATTTCCCTCCTCTAAGTGATTCAAATTTTGACCCTTGCCCGTAGATGATACACCAGGAAATTGTGACCTTGGTGTCCCGGGCATTTCAGATTCCCGTGTTAAACCATCATCCTCAATCATTGATTTTTGCTTTTCTTAGCAAGAACACCCCAAAATCCATTCTTTGACTCATCCAATCTCTTCATAGATGTATATTCATAAGCTCTTGAATCCTTGGCGGATGGAGGAACAGTTGGAGTAGGTTGAGAACGATGAGAGGAGAGATTGGAGTGACCATAAAGAGACGAGAGCGAAGAATCACGCCGCGCCGAAGAAGCTCTGATAGCTTTAGCAGCAAGTGAAGACGAAGAAGTGTCAACGCCTTCATAGTTGGAAGAAGAGGAAGAAGGTGTAAGAGGATTAGGGTTAGGGTTTCGAATTGCTTCGTTTTCGAAAATCGAAGGAGAAGACTTTGAAGTTCCGTATTGTTTTCTTCTATACGCCATGGAATCAAGCTCTCGATTTTCTGGATCCAATTGAATCTCTGCACGAAATGAAACGAACGAAAGTAGCACGGTGAGGAACCACCACTTCCAGAATCTTCGTCGGTGGTACTGGATTCGTGATGCGATCATGAACCGCTGGAAATTAATATTCAATGACGAAGTAGTAGA
TARGET=681,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3043
PRIMER_LEFT_PENALTY=0.023267
PRIMER_RIGHT_PENALTY=0.281044
PRIMER_LEFT_SEQUENCE=GACTTCCTCATTGCTCTCGTCT
PRIMER_RIGHT_SEQUENCE=TTCTTCGGGAGTTGAAGACTGT
PRIMER_LEFT=518,22
PRIMER_RIGHT=807,22
PRIMER_LEFT_TM=60.023
PRIMER_RIGHT_TM=60.281
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=290
PRIMER_PAIR_PENALTY_1=0.3043
PRIMER_LEFT_1_PENALTY=0.023267
PRIMER_RIGHT_1_PENALTY=0.281044
PRIMER_LEFT_1_SEQUENCE=GAGACTTCCTCATTGCTCTCGT
PRIMER_RIGHT_1_SEQUENCE=TTCTTCGGGAGTTGAAGACTGT
PRIMER_LEFT_1=516,22
PRIMER_RIGHT_1=807,22
PRIMER_LEFT_1_TM=60.023
PRIMER_RIGHT_1_TM=60.281
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.3043
PRIMER_LEFT_2_PENALTY=0.023267
PRIMER_RIGHT_2_PENALTY=0.281044
PRIMER_LEFT_2_SEQUENCE=AGACTTCCTCATTGCTCTCGTC
PRIMER_RIGHT_2_SEQUENCE=TTCTTCGGGAGTTGAAGACTGT
PRIMER_LEFT_2=517,22
PRIMER_RIGHT_2=807,22
PRIMER_LEFT_2_TM=60.023
PRIMER_RIGHT_2_TM=60.281
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.4302
PRIMER_LEFT_3_PENALTY=0.023267
PRIMER_RIGHT_3_PENALTY=0.406964
PRIMER_LEFT_3_SEQUENCE=GAGACTTCCTCATTGCTCTCGT
PRIMER_RIGHT_3_SEQUENCE=CTTCTTCGGGAGTTGAAGACTG
PRIMER_LEFT_3=516,22
PRIMER_RIGHT_3=808,22
PRIMER_LEFT_3_TM=60.023
PRIMER_RIGHT_3_TM=60.407
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.4302
PRIMER_LEFT_4_PENALTY=0.023267
PRIMER_RIGHT_4_PENALTY=0.406964
PRIMER_LEFT_4_SEQUENCE=GACTTCCTCATTGCTCTCGTCT
PRIMER_RIGHT_4_SEQUENCE=CTTCTTCGGGAGTTGAAGACTG
PRIMER_LEFT_4=518,22
PRIMER_RIGHT_4=808,22
PRIMER_LEFT_4_TM=60.023
PRIMER_RIGHT_4_TM=60.407
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_05753 Ps_contig_mira-and-tgicl-ass_franssen_39794
SEQUENCE=GCTCATGACAAAACTAAARAACATGCATACAATTTATATATTCCAATACAATAGGCAATGTACATCAAAAGACTCTTGTAGTCCTGCTCACACGGAGCACACACACATTAACCAGGATTTTTTTACTCATGCATAAAATACACCGAAGAAAAATAAAAATAAAAGGTTATCTACAAAAGTAACAGCCTTCAACTTTAGATGTCAATGAAAGTTTTTTTTATGTATCAAACAAGATCTGTTTTGAAGAGGCATAGTTGTATCATTGTATGTCATATCCCACCATGTCCAAAGGAGGGCATGGAAATATATTACCATCAGAACAACCTGTTCTTTTTCAGCACAGAGCACATTTGAAGATGAAAAAATAAGTGGAATCAGACCCGCACATGAAACTGGAGCAGACGGAAGAATGCAATACCTATCTTTTTTTTGGTCTACATCTTTTAGTAACAACAAGGATTTTCGATGACCTTTCTCTCTGAGATTCAGAGTCTGCATCTTGAAATGAATATAAGGCAGATCTGTTGCATGCAGCTTCCCTTGTTTCATCAACTCACTAACTGACGTATACATCAGGAACATTTGTTTTGAAGAAGGAATCAGTCTGCAACCTGCAAAACCAGAACCAAAAATTACAAAAATCTCACTTCAAATTAACCAATTCACCATTTAACTATTAACATTCCAATAAATCAAACTAATTTCAAACTCACAAGTTAGCAAACATTTCCTAATTCAAGCAGCCTTCTACATCAAACAACCAATCTTCATGACAAAGATCCAATTAACAACTCAAATCACTAATTATACTACCAAGTTCAACTAACAATTAACAAGTTAACATTTGCGAAGGATACTAAATTAACAATCAT
TARGET=98,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05754 Ps_contig_mira-and-tgicl-ass_franssen_39802
SEQUENCE=AAAGAACCTTCAATTCCGGCAACAAAAAACCTACTTTTTCCAATGTTAAATCAGCTACGGTTGTTTTTCCTAAGGCTATTTCTACTACTACTACTAGTGCTTCCAGTTCTTTTTCTGAAAGGAATGAAGCTGTTTTTCCGTTAGTTTCAACTACTTTCAATGTTCCTGTGGTTTCGAGTTGTATGGATTTATCGTCCCCCAGTAAATCCGACGGAATGTCGGTTTCGATGGATGAGACAATGTCGTCTTGTGATTCTTTTAAGAGTCCTGATATTGAGTATGTGGATAACAGCGATGTTCCGGCGGTTGATTCGATTGAGAGGAAGACTTACTGTAGTCTTAACATTTCTGATTCTGATTACCCAACAGGTAATGTTTGTAGTAGAGACATTCTTGTTGAGTTGGAAAAGGGGGAGAAAATTATTGATATTGATGATGATCATGTGGATCCTCAGCTTTGTGCATCGTTTGCTTGTGATATCTACAAGCATTTGCGTGCTTCTGAGGTAAAGAAAAGACCATCCACAAACTTCATGGAGAAAATTCAGAAGGAATATAAATCCCAGCATGCGTGCT
TARGET=82,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4861
PRIMER_LEFT_PENALTY=0.455824
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=AATCAGCTACGGTTGTTTTTCC
PRIMER_RIGHT_SEQUENCE=CATACAACTCGAAACCACAGGA
PRIMER_LEFT=48,22
PRIMER_RIGHT=184,22
PRIMER_LEFT_TM=59.544
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=0.4861
PRIMER_LEFT_1_PENALTY=0.455824
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=AATCAGCTACGGTTGTTTTTCC
PRIMER_RIGHT_1_SEQUENCE=TCCATACAACTCGAAACCACAG
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=186,22
PRIMER_LEFT_1_TM=59.544
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=139
PRIMER_PAIR_PENALTY_2=0.5164
PRIMER_LEFT_2_PENALTY=0.455824
PRIMER_RIGHT_2_PENALTY=0.060612
PRIMER_LEFT_2_SEQUENCE=AATCAGCTACGGTTGTTTTTCC
PRIMER_RIGHT_2_SEQUENCE=GACATTGTCTCATCCATCGAAA
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=243,22
PRIMER_LEFT_2_TM=59.544
PRIMER_RIGHT_2_TM=59.939
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.5303
PRIMER_LEFT_3_PENALTY=0.455824
PRIMER_RIGHT_3_PENALTY=0.074466
PRIMER_LEFT_3_SEQUENCE=AATCAGCTACGGTTGTTTTTCC
PRIMER_RIGHT_3_SEQUENCE=CTTCCTCTCAATCGAATCAACC
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=325,22
PRIMER_LEFT_3_TM=59.544
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=0.5489
PRIMER_LEFT_4_PENALTY=0.455824
PRIMER_RIGHT_4_PENALTY=0.093026
PRIMER_LEFT_4_SEQUENCE=AATCAGCTACGGTTGTTTTTCC
PRIMER_RIGHT_4_SEQUENCE=AACTCGAAACCACAGGAACATT
PRIMER_LEFT_4=48,22
PRIMER_RIGHT_4=179,22
PRIMER_LEFT_4_TM=59.544
PRIMER_RIGHT_4_TM=59.907
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=132
=
PRIMER_SEQUENCE_ID=SSR_05755 Ps_contig_mira-and-tgicl-ass_franssen_39805
SEQUENCE=CGGTGGTGGTGGTGGCGTTGGTGNTNNNGYTTNCNNTRGNATTGGGAAGATTTCAGGACATCCGAGAGAGTAGTACTTGCATGGTAACTCGAGTGATTCGGCAACCTTCTCTAGTGCCAGACACCTGATATCTCCAAGCTCTTGTCTACAAGTCGGACATCGATTGTGCACTCTTGTTTTACATGTGGAACACAATGTGTGGCCGTTGTGGCACTGATGAATTGGAGGATACATTGAATTGGTACAGACGGGACATTCCAACAACTCATGAACACTTGTAGCAGGAGCAATAGCTGTAGAACCAATAACATGATTAATGTTATTAGTGTTTCCTCCATTACGAGGTTTCAAGGAAGAAAACTCAGAGTGATGAGGATGATGTTGATGTGGATGTTGATATGGATGAGGATGAAGAATACGGTGTTGGATCTCATCTTCATCCATTCCATCGGAAGATGAAACACACTCAATGCTATCCAAGTCCATGATAATCAAAACTGAACCAAATAC
TARGET=2,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05756 Ps_contig_mira-and-tgicl-ass_franssen_39808
SEQUENCE=ACGACGCCGTTTCCCCATAATTACCTTCTTGCTCTTTATTTACGTTCCGTTTCGTTTTTGTTTGTACACATAATTACCCCCTTGTCCATTACGACACCGTTTGCGAACCATACGACGCNGTTTGAGGGTACGGTGCCGTAGGATAATACGATCCTTGTTGCTGAGGTGGAGGTAGGAGCACCGTATGGGTAACCATACACCGACGAAGCAGAGGAATAATCCCGCGAATTTGATTCTGGAGGTGGAACACCATAAGGAGGAGGATAATTCGCACCATGTGCATGATAACCAATCTCTCTAACCCCCACCTTAACGTCAACCTTTCCTTGTGGTCTTCCAGAAGGTCGTTTCAATGTGAGCGTGCGATTCACACGCTCGCCGATTCCAACTTCAAGAACATCAACAAGCTGAAGCCGAGCCGAGCCGATGAGTTTCTTTGTATCTTCTTCAAATCCAGCGTGAACGACGTCGATGTATAAAGTGAGATCCTCGAATGGTTTTGGTGGTAAAGGAAGGATAAGTGGTTGATTCCATTCAGCTTCGGTGTCGCCATTTGGGTCTACATTGGTTGTGAATTTATTGACTTGATCAACCCAAACGACGGCGTATGGTTGGTTAGCGCCGTGGCGCCAGTTGACGTTCTTGAGTGATTTTGCTGATGTGAGATTCAGATCGAGTTCGTAGCGAGATGATGCCATTTTTGGATGACAGTGAGAGTTATGAAGTTGAAGATGAGAGTGTGGTAGTTAGGGTTTTATAAGGGTTGTTAGAGATTTATTCCGTTTTGGGTCTTTCGTTTTTTTAGGGTTATTTATACTAAATTTCG
TARGET=412,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0329
PRIMER_LEFT_PENALTY=0.028536
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=CCTTAACGTCAACCTTTCCTTG
PRIMER_RIGHT_SEQUENCE=AGCTGAATGGAATCAACCACTT
PRIMER_LEFT=307,22
PRIMER_RIGHT=539,22
PRIMER_LEFT_TM=60.029
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=233
PRIMER_PAIR_PENALTY_1=0.0329
PRIMER_LEFT_1_PENALTY=0.028536
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=CCTTAACGTCAACCTTTCCTTG
PRIMER_RIGHT_1_SEQUENCE=AAGCTGAATGGAATCAACCACT
PRIMER_LEFT_1=307,22
PRIMER_RIGHT_1=540,22
PRIMER_LEFT_1_TM=60.029
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.1172
PRIMER_LEFT_2_PENALTY=0.028536
PRIMER_RIGHT_2_PENALTY=0.088662
PRIMER_LEFT_2_SEQUENCE=CCTTAACGTCAACCTTTCCTTG
PRIMER_RIGHT_2_SEQUENCE=TCCTTTACCACCAAAACCATTC
PRIMER_LEFT_2=307,22
PRIMER_RIGHT_2=512,22
PRIMER_LEFT_2_TM=60.029
PRIMER_RIGHT_2_TM=60.089
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.1749
PRIMER_LEFT_3_PENALTY=0.170538
PRIMER_RIGHT_3_PENALTY=0.004359
PRIMER_LEFT_3_SEQUENCE=TAACCAATCTCTCTAACCCCCA
PRIMER_RIGHT_3_SEQUENCE=AGCTGAATGGAATCAACCACTT
PRIMER_LEFT_3=285,22
PRIMER_RIGHT_3=539,22
PRIMER_LEFT_3_TM=59.829
PRIMER_RIGHT_3_TM=60.004
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.1749
PRIMER_LEFT_4_PENALTY=0.170538
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=TAACCAATCTCTCTAACCCCCA
PRIMER_RIGHT_4_SEQUENCE=AAGCTGAATGGAATCAACCACT
PRIMER_LEFT_4=285,22
PRIMER_RIGHT_4=540,22
PRIMER_LEFT_4_TM=59.829
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=11.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=256
=
PRIMER_SEQUENCE_ID=SSR_05757 Ps_contig_mira-and-tgicl-ass_franssen_39810
SEQUENCE=GTGCTAGCTGTGACACCACTTCTACACCTCTTTGGAGAAATGGTCCTCGTGGCCCAAAGTCACTATGCAATGCTTGTGGGATTAGATACAAGAAGGAAGAACGAAGAGCGAATGCTGCCACGTCGGCAGCGACAACCGGCGGTGTAATGGAGACAGGCAGCATCTACAGCAGCAACAACAACAACGGTTCGTGGTACGGACAACCGCAGAGTCAAATGTATGGGAACGAGTTACGGTTCATGGAAGATTCAGATGTAGAATCAGAGAATGGGATTCCTAACTTTCTTTCTTGGAGACTCAACACGAGCCTTGTTCATGATTACACTAGATGAAAGAAGCGTTAATGGAATGAATACGATAATAGTATAACAGAAAGAAGAAAAAGGATGATGTAGATGTTTTACGATGATGATGATGCTGATGATGTAGATGTTTTTGATTGCGATGGTTATGATGGTCTGGTCAACGTTATTTTACTTTTTTGACCTTTTTCTTTTTCTATCTGGTTCTTTTCTTTTCTCTGTCTTCTTGCTTAAGAATATGGAGATAGAATCGGATAAATTCTATAGAGTATGTTCTTAACTTGTTTGTTTTTGTTTTATTTATTTATTAATGTATTCTTTTTTTGTGACTTTATCTTTTGAGTTATTAGTACTTCGAAANCTCANCCTTTCTGTTTCTACC
TARGET=173,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1439
PRIMER_LEFT_PENALTY=0.014792
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=CTATGCAATGCTTGTGGGATTA
PRIMER_RIGHT_SEQUENCE=CCGTAACTCGTTCCCATACATT
PRIMER_LEFT=62,22
PRIMER_RIGHT=235,22
PRIMER_LEFT_TM=59.985
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=174
PRIMER_PAIR_PENALTY_1=0.1460
PRIMER_LEFT_1_PENALTY=0.016921
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=GTGCTAGCTGTGACACCACTTC
PRIMER_RIGHT_1_SEQUENCE=CCGTAACTCGTTCCCATACATT
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=235,22
PRIMER_LEFT_1_TM=59.983
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.1820
PRIMER_LEFT_2_PENALTY=0.014792
PRIMER_RIGHT_2_PENALTY=0.167242
PRIMER_LEFT_2_SEQUENCE=TATGCAATGCTTGTGGGATTAG
PRIMER_RIGHT_2_SEQUENCE=ATCTGAATCTTCCATGAACCGT
PRIMER_LEFT_2=63,22
PRIMER_RIGHT_2=253,22
PRIMER_LEFT_2_TM=59.985
PRIMER_RIGHT_2_TM=59.833
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.1820
PRIMER_LEFT_3_PENALTY=0.014792
PRIMER_RIGHT_3_PENALTY=0.167242
PRIMER_LEFT_3_SEQUENCE=CTATGCAATGCTTGTGGGATTA
PRIMER_RIGHT_3_SEQUENCE=ATCTGAATCTTCCATGAACCGT
PRIMER_LEFT_3=62,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=59.985
PRIMER_RIGHT_3_TM=59.833
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=9.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=0.1842
PRIMER_LEFT_4_PENALTY=0.016921
PRIMER_RIGHT_4_PENALTY=0.167242
PRIMER_LEFT_4_SEQUENCE=GTGCTAGCTGTGACACCACTTC
PRIMER_RIGHT_4_SEQUENCE=ATCTGAATCTTCCATGAACCGT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=253,22
PRIMER_LEFT_4_TM=59.983
PRIMER_RIGHT_4_TM=59.833
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_05758 Ps_contig_mira-and-tgicl-ass_franssen_39810
SEQUENCE=GTGCTAGCTGTGACACCACTTCTACACCTCTTTGGAGAAATGGTCCTCGTGGCCCAAAGTCACTATGCAATGCTTGTGGGATTAGATACAAGAAGGAAGAACGAAGAGCGAATGCTGCCACGTCGGCAGCGACAACCGGCGGTGTAATGGAGACAGGCAGCATCTACAGCAGCAACAACAACAACGGTTCGTGGTACGGACAACCGCAGAGTCAAATGTATGGGAACGAGTTACGGTTCATGGAAGATTCAGATGTAGAATCAGAGAATGGGATTCCTAACTTTCTTTCTTGGAGACTCAACACGAGCCTTGTTCATGATTACACTAGATGAAAGAAGCGTTAATGGAATGAATACGATAATAGTATAACAGAAAGAAGAAAAAGGATGATGTAGATGTTTTACGATGATGATGATGCTGATGATGTAGATGTTTTTGATTGCGATGGTTATGATGGTCTGGTCAACGTTATTTTACTTTTTTGACCTTTTTCTTTTTCTATCTGGTTCTTTTCTTTTCTCTGTCTTCTTGCTTAAGAATATGGAGATAGAATCGGATAAATTCTATAGAGTATGTTCTTAACTTGTTTGTTTTTGTTTTATTTATTTATTAATGTATTCTTTTTTTGTGACTTTATCTTTTGAGTTATTAGTACTTCGAAANCTCANCCTTTCTGTTTCTACC
TARGET=405,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4120
PRIMER_LEFT_PENALTY=0.129103
PRIMER_RIGHT_PENALTY=0.282868
PRIMER_LEFT_SEQUENCE=AATGTATGGGAACGAGTTACGG
PRIMER_RIGHT_SEQUENCE=GTTGACCAGACCATCATAACCA
PRIMER_LEFT=214,22
PRIMER_RIGHT=466,22
PRIMER_LEFT_TM=60.129
PRIMER_RIGHT_TM=59.717
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.4501
PRIMER_LEFT_1_PENALTY=0.167242
PRIMER_RIGHT_1_PENALTY=0.282868
PRIMER_LEFT_1_SEQUENCE=ACGGTTCATGGAAGATTCAGAT
PRIMER_RIGHT_1_SEQUENCE=GTTGACCAGACCATCATAACCA
PRIMER_LEFT_1=232,22
PRIMER_RIGHT_1=466,22
PRIMER_LEFT_1_TM=59.833
PRIMER_RIGHT_1_TM=59.717
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.4528
PRIMER_LEFT_2_PENALTY=0.129103
PRIMER_RIGHT_2_PENALTY=0.323721
PRIMER_LEFT_2_SEQUENCE=AATGTATGGGAACGAGTTACGG
PRIMER_RIGHT_2_SEQUENCE=TGACCAGACCATCATAACCATC
PRIMER_LEFT_2=214,22
PRIMER_RIGHT_2=464,22
PRIMER_LEFT_2_TM=60.129
PRIMER_RIGHT_2_TM=59.676
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.9000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.4585
PRIMER_LEFT_3_PENALTY=0.175676
PRIMER_RIGHT_3_PENALTY=0.282868
PRIMER_LEFT_3_SEQUENCE=GACAACCGCAGAGTCAAATGTA
PRIMER_RIGHT_3_SEQUENCE=GTTGACCAGACCATCATAACCA
PRIMER_LEFT_3=198,22
PRIMER_RIGHT_3=466,22
PRIMER_LEFT_3_TM=60.176
PRIMER_RIGHT_3_TM=59.717
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=269
PRIMER_PAIR_PENALTY_4=0.4994
PRIMER_LEFT_4_PENALTY=0.175676
PRIMER_RIGHT_4_PENALTY=0.323721
PRIMER_LEFT_4_SEQUENCE=GACAACCGCAGAGTCAAATGTA
PRIMER_RIGHT_4_SEQUENCE=TGACCAGACCATCATAACCATC
PRIMER_LEFT_4=198,22
PRIMER_RIGHT_4=464,22
PRIMER_LEFT_4_TM=60.176
PRIMER_RIGHT_4_TM=59.676
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_05759 Ps_contig_mira-and-tgicl-ass_franssen_39810
SEQUENCE=GTGCTAGCTGTGACACCACTTCTACACCTCTTTGGAGAAATGGTCCTCGTGGCCCAAAGTCACTATGCAATGCTTGTGGGATTAGATACAAGAAGGAAGAACGAAGAGCGAATGCTGCCACGTCGGCAGCGACAACCGGCGGTGTAATGGAGACAGGCAGCATCTACAGCAGCAACAACAACAACGGTTCGTGGTACGGACAACCGCAGAGTCAAATGTATGGGAACGAGTTACGGTTCATGGAAGATTCAGATGTAGAATCAGAGAATGGGATTCCTAACTTTCTTTCTTGGAGACTCAACACGAGCCTTGTTCATGATTACACTAGATGAAAGAAGCGTTAATGGAATGAATACGATAATAGTATAACAGAAAGAAGAAAAAGGATGATGTAGATGTTTTACGATGATGATGATGCTGATGATGTAGATGTTTTTGATTGCGATGGTTATGATGGTCTGGTCAACGTTATTTTACTTTTTTGACCTTTTTCTTTTTCTATCTGGTTCTTTTCTTTTCTCTGTCTTCTTGCTTAAGAATATGGAGATAGAATCGGATAAATTCTATAGAGTATGTTCTTAACTTGTTTGTTTTTGTTTTATTTATTTATTAATGTATTCTTTTTTTGTGACTTTATCTTTTGAGTTATTAGTACTTCGAAANCTCANCCTTTCTGTTTCTACC
TARGET=598,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05760 Ps_contig_mira-and-tgicl-ass_franssen_39823
SEQUENCE=AAGGATAAAACATTAACAGAAGAAACATGGACATTAACACATCCATCTTCGTCCATATGAATGACATTGTTGGATCCATCATTGTTGTTGTTGTTCTTATTATCGCTTATTGGAGTTGCAATTCCAACCTCAGATTCTGGTCTCTTCCTCAAATTCTTGTTATTGTGCATCCAAACTTTGAAAACCCCTCTTGAAACACCAACGTCATTGCAGAATTTCTGAACCATTCCATCATCACCCTTTTGCATTCTCCAACCCAGTTTTTCAGAAAATCCATACATCATCTCTTTCTGCTCCTTGCTGTAATTTTGTCCTGTACCTCTTTTTCCCACAGCTTGAGTTGYTGTCTGTTGTTGTTGTTGGGGTTTTCGAGTTTAATCACTGATGAAGAAAAATTGAGATTTCTTTGATGGGGTTGTTGGTGTTGTTGTTGGTGTTGGTGTTGATCATGATCAGATTGTGGTGGTGTTGAATAAGCATTTCCAAGTGCTAGCAGCATGTGAGGGGCTGAAGAGTGATGATGATAAGGTGGTAAATGTGAGAGTGGCGGTGGAGAAGATGGGCTTGACGTTGGGCTTGGGCTTGACGTTGGGCTTGTGCTTTGACTTGGGCTCGTGCTTTGACTGATTGGGCCACGGTGTGGTAGTTGTAATGGTGGCGGTGGTTGTGGTGGTGGAACTGTTGTTGATGACTAGGAAGTAGTAAGAAATGCAGTTAAGAAAAGTGGGGTTTGTGGTGGCGTTGGTGGTGGTGTTGGTGGTGGCGTTGACGGTGGTGGTGTTGGTGGTGGGGACGATGTGTTGTTCTTGAGGTTCACGGCGGTGGAAATTCCGG
TARGET=82,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05761 Ps_contig_mira-and-tgicl-ass_franssen_39823
SEQUENCE=AAGGATAAAACATTAACAGAAGAAACATGGACATTAACACATCCATCTTCGTCCATATGAATGACATTGTTGGATCCATCATTGTTGTTGTTGTTCTTATTATCGCTTATTGGAGTTGCAATTCCAACCTCAGATTCTGGTCTCTTCCTCAAATTCTTGTTATTGTGCATCCAAACTTTGAAAACCCCTCTTGAAACACCAACGTCATTGCAGAATTTCTGAACCATTCCATCATCACCCTTTTGCATTCTCCAACCCAGTTTTTCAGAAAATCCATACATCATCTCTTTCTGCTCCTTGCTGTAATTTTGTCCTGTACCTCTTTTTCCCACAGCTTGAGTTGYTGTCTGTTGTTGTTGTTGGGGTTTTCGAGTTTAATCACTGATGAAGAAAAATTGAGATTTCTTTGATGGGGTTGTTGGTGTTGTTGTTGGTGTTGGTGTTGATCATGATCAGATTGTGGTGGTGTTGAATAAGCATTTCCAAGTGCTAGCAGCATGTGAGGGGCTGAAGAGTGATGATGATAAGGTGGTAAATGTGAGAGTGGCGGTGGAGAAGATGGGCTTGACGTTGGGCTTGGGCTTGACGTTGGGCTTGTGCTTTGACTTGGGCTCGTGCTTTGACTGATTGGGCCACGGTGTGGTAGTTGTAATGGTGGCGGTGGTTGTGGTGGTGGAACTGTTGTTGATGACTAGGAAGTAGTAAGAAATGCAGTTAAGAAAAGTGGGGTTTGTGGTGGCGTTGGTGGTGGTGTTGGTGGTGGCGTTGACGGTGGTGGTGTTGGTGGTGGGGACGATGTGTTGTTCTTGAGGTTCACGGCGGTGGAAATTCCGG
TARGET=349,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05762 Ps_contig_mira-and-tgicl-ass_franssen_39826
SEQUENCE=ATAGATTCATTGTGGGTCTAGGGCAGTAGGGTAGGAGAAACGAGTGGAGGACGAAATCGGAAATGATATTTCNGGCTACATTCCACGTTATTTGCATTGTCTTCTTTCTACTTGGAAAGCGCCGAACAAAATTTTAGATATTATTATTCACCAAATACTCACTCATCGAGAAGAGTCAAAATTCGCTCACTGAGTCACAAGAAAGTTAGTAGAAAAAACAATGGAAGAGGAAGGAATAGGGTTAGTGTTAGCCAGAGCCACAGAACTTCGATTCAAGATCACCAACTGCATTCACCACAATCCCAAATCTTCTCCCAATGCTCTCGGCGACGACGAAGAAGAAGACGATGATGCTACTGATAGGCTTCTCAATATTTGCGATGCACTCGAAGCTTTGGAGACTCAGCTTTCTTCTCTGCAGGTTCTGCAACAACAACAACGGTACGAAAGAGAAATTGCCTTGGCTGAGATTGAAAACAGTCGCAAAATGTTAATTAATAAACTCAAAGAGTATAAAGGTAAAGATTTGGAAGTGATACATGAAGCTTCCACTTTTGCCAGTGAAACAGTGGAGCCAAACAATGATCTGCTACTTCCTCCTTATCCAACCCGTCCTCCATATTCTATGTCTCTTGACAAGGAATATCTGTCACAGAACCCTTCTGTCAACAAGTCTGGTCGCAATGGATTAATCACACTTGATAACGTGATCGAGCCTAAAAAGAATCCAAATGAAAAAGAGAAAAATCATGTTGAAGATGGAGGCAAAAGTTCAAGAAAAGGGTTATGGTGTGTTATAACTTCTGCAGCAAAAACAATGCTCACAGTTGTTGGCGTGGTATCAATATTGAGTTTGTCTGGATTTGGTCCTAATTTGCACAAGATTCGTTTCAATGCACAAGGCAGGCATCACAGACTAGAAAACGAGAACGTGACATCTACCATTGAGAGATTGTGTCCACCTGGGAGAGTTTTGGTGATGGAAAACGGGGAGTCTCGATGTCTAGTGAAAGAGAGAGTTGAAGTTCCATTTTCATCTGTTGCTGCAACACCTGATATAAACTATGGAAGTGGTTAAGATTAGCATTTATTCTCCGCATTGTATATCATTTCCCCCAATTTTATGTTCTAATTTTTGTTGATATGATGTTGAAAAAATTACCTTGGTGGTTGGTTTCATAGTCTTTTTTAAAATAAAAAAAAAAAAAAAAANAAAAAAATACTCTGTCGTAGAACTACCACTGTCCTTCGTAGAACG
TARGET=428,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2420
PRIMER_LEFT_PENALTY=0.188254
PRIMER_RIGHT_PENALTY=0.053733
PRIMER_LEFT_SEQUENCE=ACTCGAAGCTTTGGAGACTCAG
PRIMER_RIGHT_SEQUENCE=CGACCAGACTTGTTGACAGAAG
PRIMER_LEFT=384,22
PRIMER_RIGHT=680,22
PRIMER_LEFT_TM=60.188
PRIMER_RIGHT_TM=59.946
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.3764
PRIMER_LEFT_1_PENALTY=0.322707
PRIMER_RIGHT_1_PENALTY=0.053733
PRIMER_LEFT_1_SEQUENCE=CTCAGCTTTCTTCTCTGCAGGT
PRIMER_RIGHT_1_SEQUENCE=CGACCAGACTTGTTGACAGAAG
PRIMER_LEFT_1=401,22
PRIMER_RIGHT_1=680,22
PRIMER_LEFT_1_TM=60.323
PRIMER_RIGHT_1_TM=59.946
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.4603
PRIMER_LEFT_2_PENALTY=0.322707
PRIMER_RIGHT_2_PENALTY=0.137550
PRIMER_LEFT_2_SEQUENCE=CTCAGCTTTCTTCTCTGCAGGT
PRIMER_RIGHT_2_SEQUENCE=ATTAATCCATTGCGACCAGACT
PRIMER_LEFT_2=401,22
PRIMER_RIGHT_2=692,22
PRIMER_LEFT_2_TM=60.323
PRIMER_RIGHT_2_TM=59.862
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=292
PRIMER_PAIR_PENALTY_3=0.4702
PRIMER_LEFT_3_PENALTY=0.050443
PRIMER_RIGHT_3_PENALTY=0.419726
PRIMER_LEFT_3_SEQUENCE=GTTAGCCAGAGCCACAGAACTT
PRIMER_RIGHT_3_SEQUENCE=TTGCGACTGTTTTCAATCTCAG
PRIMER_LEFT_3=246,22
PRIMER_RIGHT_3=485,22
PRIMER_LEFT_3_TM=59.950
PRIMER_RIGHT_3_TM=60.420
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=0.4937
PRIMER_LEFT_4_PENALTY=0.073989
PRIMER_RIGHT_4_PENALTY=0.419726
PRIMER_LEFT_4_SEQUENCE=TAGTGTTAGCCAGAGCCACAGA
PRIMER_RIGHT_4_SEQUENCE=TTGCGACTGTTTTCAATCTCAG
PRIMER_LEFT_4=242,22
PRIMER_RIGHT_4=485,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=60.420
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=244
=
PRIMER_SEQUENCE_ID=SSR_05763 Ps_contig_mira-and-tgicl-ass_franssen_39830
SEQUENCE=ACTTTTTAGTAACAAATAGNANTTTTTCCAACCTATAAATAACATTATTACTCATTATCAAAGTACAACGAGTCATAATCTTCACGTAATTCTTATTCTTGCCTTACATAACAATTCATAGAGTATAATTACTCAGAAAAAACAAAGCAAACAAACCAACATAGCAATCTTAATTTGTTCTCTTGTTTTGAAAGCCACAAACATAAACAACACAATACAAACCAAATTTCTCCCTTATTGTGCTTTCCTTGTCAATGATATGGTGAAATAAACAGTCTGCATTTTCTCCCTGGACATTTGATCCACCACGATTTCGTGATATCGTTGAAAAACATCGTCGATTATAGCTTCACCAAAGTGACTAACCAGCAAAGGTTCAAACACAGCCCTTATCGTTTGCGCAACATTGTATCCACTGTCATGTAATGATTCACCAGAATTATTTAAATCTACTTCTGAAAACTCCACATGGTTGATGGCAAATGATCCTTCTTCGAGAATACTCGACTTCACTTCACTTCGAGATGGATAATAATTTGGAATATTGAAAGTATTCACTTTATCTTCTTCAATAAGTCCCTTTAAGACCATGTGATTAAGAGCAGTGGCGAGAAGATCCAAAAGGTCGCAACTTTCCTTACTGAATGGATCATCGCCTCTTCTTCCTAAAATCGATAGAACCATGCGACCTCCTTCGACTATTTCCTCAGCGCGACACTTGATGAAAACGGAAAAGTCTCTTTCAAATTGCTCATAGTAAGCTTTAATAACATTCGAAGGACTCGTTTCGTTGATGTAAATGGCACCCTTACTATTCTCTATTCCTTCCGGAACCTTAGACAGCCAATGAACGCTATAAGAAGAATGGACGAAATGCAGACTCCGGTCAGGAAAAACTCTACCATAGAAAGAACCAGGTACTCCAAAGAAATAGCAGCGATCTATTTCAGTTTTGGTTTCATCTCGTAGCTTCTCTTTGAAGCTATCGAGTGACCTAAAGACGCTGTTGAAGTCATTTCCTGCAAGATCATTCAAATATATTTTGTATTCGGGAGAAGAATGATTCAGCTTTTTGCAAAGATTCTCCACAACATGGATAACTTCTGACACCACAGATAAAGTGTTTGGTCCACAAGAACAACCCAAATCTGCAATTGCAAGGCTTCTAGGAAGGTGTGCTGGAGTATAGACTGGTTATGGCTTCCTCTCTTATGAGCTTTGTAGAAGAAATCACATTTCCCTGAAAAATAGATTGAGTTGTTTGCATAACTTTCTTCTCCATCACCTCCTTTCATGTGCATTCCCTGCGCTACTTCCATTCTTGAGGCTTTATGTCTCACTTACTTTCTGAATGTTTAGATTGTTGTGAGTTCCCCG
TARGET=507,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3326
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.324355
PRIMER_LEFT_SEQUENCE=TTCTGAAAACTCCACATGGTTG
PRIMER_RIGHT_SEQUENCE=TTTTAGGAAGAAGAGGCGATGA
PRIMER_LEFT=453,22
PRIMER_RIGHT=670,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.324
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=0.3478
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.339582
PRIMER_LEFT_1_SEQUENCE=TTCTGAAAACTCCACATGGTTG
PRIMER_RIGHT_1_SEQUENCE=GTCGCATGGTTCTATCGATTTT
PRIMER_LEFT_1=453,22
PRIMER_RIGHT_1=688,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.340
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.4795
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.471231
PRIMER_LEFT_2_SEQUENCE=TTCTGAAAACTCCACATGGTTG
PRIMER_RIGHT_2_SEQUENCE=AGGCGATGATCCATTCAGTAAG
PRIMER_LEFT_2=453,22
PRIMER_RIGHT_2=657,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.471
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.5041
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.495821
PRIMER_LEFT_3_SEQUENCE=TTCTGAAAACTCCACATGGTTG
PRIMER_RIGHT_3_SEQUENCE=GCTGAGGAAATAGTCGAAGGAG
PRIMER_LEFT_3=453,22
PRIMER_RIGHT_3=710,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.504
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=0.5305
PRIMER_LEFT_4_PENALTY=0.008257
PRIMER_RIGHT_4_PENALTY=0.522246
PRIMER_LEFT_4_SEQUENCE=TTCTGAAAACTCCACATGGTTG
PRIMER_RIGHT_4_SEQUENCE=CTTCTCGCCACTGCTCTTAATC
PRIMER_LEFT_4=453,22
PRIMER_RIGHT_4=614,22
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=60.522
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_05764 Ps_contig_mira-and-tgicl-ass_franssen_39836
SEQUENCE=GTGCAATAGACGATCCCAACGTTACATAATACCTCTGCAACAAGAAAATTANCCNAAAAAAAAAGAAAGAAACAATTTATTATAATTAATGTTTCAAAAATTAACAAAATNCTCTATAACTTTTTCGTATTCAAAAATTACCTTTTGGTTTAGACCAAAGTATCTTAATTTTCATATCTAGTATAAACCTTAATCATAAAGTGCTAAAGTGCCATAATTCCTGTTGAAAACATTTGATCCTTTGTGTATGAAGAACTTGAAGCTTCTGATCTACACTTCTCTTTTCTCTCATCCATCATTATTTGCTTCGAACGACACTCTGTGCTACAAAATCCTTTCTCYCCTCTGTACATGAATATGTCTTTGCCATCTAGATTCATTTTGCATAAATGACAGGAATTGAGAAAATCCAAAGTGAGAAACGACGGTTCAGCTTCCAGAAAAGTTTGTGTTGGAGGGGATCTTCGAAAAACACCAACGTTGTTGTTGTTGTTGTTGTTTTTTGAGGTATTATTATTATTATAATAATAATCATGTCTCAAAATTCCACTTTCTTCACCCGCATCATAAAAAACCTTAAGTGACGGGTTTGTTGGATTCATCACGGTATGTAACGTACGTGTAATCTTCAGGACTTCCAACAAGAATCGCGTTTTCAAATAATCGATTCTGTTGATGCTTTTGAATTGGGATTGGTGATGTACAAACATGTTGTTTTGGAACAACCTCGTATCCGGTTTCCTCGGATTTATCAAGAGCCACCACAATTCCAAGACCAACACCACCACCACCAAATTTCGGTGATTGAATTTGCATCCTTGAATTCATTTCAAACGGGCTTCTTGGACTTCCGATTGTGTCCAAAAGCGCGGTGGCAGTTTCACCGAACACTAACAATTCTGAAAATTTTCGGATCAAGTGCTGGGATCTCTTACCCAACATGCTGAAAACAATGATCTATAAAATTTTGTGAGTTATATTAGAATTGTTGTTTGAAGAAAATGAGAATGAATTTGTGAAGGGTTTTTAATGAGAGTGATGGCTTTTTATAAAAGCGATTTTGTTATGGGTTGAAAAATAATGGATGGTTTTGGTATT
TARGET=480,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05765 Ps_contig_mira-and-tgicl-ass_franssen_39836
SEQUENCE=GTGCAATAGACGATCCCAACGTTACATAATACCTCTGCAACAAGAAAATTANCCNAAAAAAAAAGAAAGAAACAATTTATTATAATTAATGTTTCAAAAATTAACAAAATNCTCTATAACTTTTTCGTATTCAAAAATTACCTTTTGGTTTAGACCAAAGTATCTTAATTTTCATATCTAGTATAAACCTTAATCATAAAGTGCTAAAGTGCCATAATTCCTGTTGAAAACATTTGATCCTTTGTGTATGAAGAACTTGAAGCTTCTGATCTACACTTCTCTTTTCTCTCATCCATCATTATTTGCTTCGAACGACACTCTGTGCTACAAAATCCTTTCTCYCCTCTGTACATGAATATGTCTTTGCCATCTAGATTCATTTTGCATAAATGACAGGAATTGAGAAAATCCAAAGTGAGAAACGACGGTTCAGCTTCCAGAAAAGTTTGTGTTGGAGGGGATCTTCGAAAAACACCAACGTTGTTGTTGTTGTTGTTGTTTTTTGAGGTATTATTATTATTATAATAATAATCATGTCTCAAAATTCCACTTTCTTCACCCGCATCATAAAAAACCTTAAGTGACGGGTTTGTTGGATTCATCACGGTATGTAACGTACGTGTAATCTTCAGGACTTCCAACAAGAATCGCGTTTTCAAATAATCGATTCTGTTGATGCTTTTGAATTGGGATTGGTGATGTACAAACATGTTGTTTTGGAACAACCTCGTATCCGGTTTCCTCGGATTTATCAAGAGCCACCACAATTCCAAGACCAACACCACCACCACCAAATTTCGGTGATTGAATTTGCATCCTTGAATTCATTTCAAACGGGCTTCTTGGACTTCCGATTGTGTCCAAAAGCGCGGTGGCAGTTTCACCGAACACTAACAATTCTGAAAATTTTCGGATCAAGTGCTGGGATCTCTTACCCAACATGCTGAAAACAATGATCTATAAAATTTTGTGAGTTATATTAGAATTGTTGTTTGAAGAAAATGAGAATGAATTTGTGAAGGGTTTTTAATGAGAGTGATGGCTTTTTATAAAAGCGATTTTGTTATGGGTTGAAAAATAATGGATGGTTTTGGTATT
TARGET=509,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05766 Ps_contig_mira-and-tgicl-ass_franssen_39836
SEQUENCE=GTGCAATAGACGATCCCAACGTTACATAATACCTCTGCAACAAGAAAATTANCCNAAAAAAAAAGAAAGAAACAATTTATTATAATTAATGTTTCAAAAATTAACAAAATNCTCTATAACTTTTTCGTATTCAAAAATTACCTTTTGGTTTAGACCAAAGTATCTTAATTTTCATATCTAGTATAAACCTTAATCATAAAGTGCTAAAGTGCCATAATTCCTGTTGAAAACATTTGATCCTTTGTGTATGAAGAACTTGAAGCTTCTGATCTACACTTCTCTTTTCTCTCATCCATCATTATTTGCTTCGAACGACACTCTGTGCTACAAAATCCTTTCTCYCCTCTGTACATGAATATGTCTTTGCCATCTAGATTCATTTTGCATAAATGACAGGAATTGAGAAAATCCAAAGTGAGAAACGACGGTTCAGCTTCCAGAAAAGTTTGTGTTGGAGGGGATCTTCGAAAAACACCAACGTTGTTGTTGTTGTTGTTGTTTTTTGAGGTATTATTATTATTATAATAATAATCATGTCTCAAAATTCCACTTTCTTCACCCGCATCATAAAAAACCTTAAGTGACGGGTTTGTTGGATTCATCACGGTATGTAACGTACGTGTAATCTTCAGGACTTCCAACAAGAATCGCGTTTTCAAATAATCGATTCTGTTGATGCTTTTGAATTGGGATTGGTGATGTACAAACATGTTGTTTTGGAACAACCTCGTATCCGGTTTCCTCGGATTTATCAAGAGCCACCACAATTCCAAGACCAACACCACCACCACCAAATTTCGGTGATTGAATTTGCATCCTTGAATTCATTTCAAACGGGCTTCTTGGACTTCCGATTGTGTCCAAAAGCGCGGTGGCAGTTTCACCGAACACTAACAATTCTGAAAATTTTCGGATCAAGTGCTGGGATCTCTTACCCAACATGCTGAAAACAATGATCTATAAAATTTTGTGAGTTATATTAGAATTGTTGTTTGAAGAAAATGAGAATGAATTTGTGAAGGGTTTTTAATGAGAGTGATGGCTTTTTATAAAAGCGATTTTGTTATGGGTTGAAAAATAATGGATGGTTTTGGTATT
TARGET=780,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05767 Ps_contig_mira-and-tgicl-ass_franssen_39851
SEQUENCE=TTGTCAGCAGTGTCGTAGAATGATTGAGCTTACTCAAGGTTGTTACCACATGACATGCTGGTGTGGTCACGAATTCTGCTATTCTTGTGGCGCAGAATACAGGAATGGCCAACAAACATGTCAATGTGCATTTTGGGACGAAGACAGCTTGACAAACTCCCTTCAAGAATCAGAACAATGGGCATGGGAGACTTCTATGATCATGGATGCGTACTCAGATCAAGAACGGTCACAACTCGCGCTTATACAACGGTTCCTTGACGGCGGTTTTAGTCTCAGTGACCACAATCCCTACCAATCTCCTCCTCCTCCTCCTCCTCAATGTACAGAATCCTTTGTAGACCCCTTGAAGGATCTTCATCAACTTCCATGGCTAGAGAGATTTGTTTCTGTGATAAGTGACAACTACTATGAAGATTACATGCAATAAAATTCTTCACATCATACAATGCATGTTTGGATTGTTGGTATGTTTGGCAGAATCACACTTTTGCCATGATTTTGGTAAAAGCTACATTTTTGTGCTTCAACAGAATTGAACGCATTGCTGTAATTTCGACAAAATCACCGTATCAGTGTGATTTTGTCAAACTCATTGACAATTCAAACATGCAAATAGTTAGTTCATCAACTTTATACAATCACATGCAAGTAAAGTTTATGACC
TARGET=300,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0679
PRIMER_LEFT_PENALTY=0.036173
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=AGTCTCAGTGACCACAATCCCT
PRIMER_RIGHT_SEQUENCE=AGCCATGGAAGTTGATGAAGAT
PRIMER_LEFT=271,22
PRIMER_RIGHT=374,22
PRIMER_LEFT_TM=60.036
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=104
PRIMER_PAIR_PENALTY_1=0.1475
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.031769
PRIMER_LEFT_1_SEQUENCE=AATACAGGAATGGCCAACAAAC
PRIMER_RIGHT_1_SEQUENCE=AGCCATGGAAGTTGATGAAGAT
PRIMER_LEFT_1=95,22
PRIMER_RIGHT_1=374,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=0.1531
PRIMER_LEFT_2_PENALTY=0.036173
PRIMER_RIGHT_2_PENALTY=0.116923
PRIMER_LEFT_2_SEQUENCE=AGTCTCAGTGACCACAATCCCT
PRIMER_RIGHT_2_SEQUENCE=CATACCAACAATCCAAACATGC
PRIMER_LEFT_2=271,22
PRIMER_RIGHT_2=471,22
PRIMER_LEFT_2_TM=60.036
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.1690
PRIMER_LEFT_3_PENALTY=0.036173
PRIMER_RIGHT_3_PENALTY=0.132857
PRIMER_LEFT_3_SEQUENCE=AGTCTCAGTGACCACAATCCCT
PRIMER_RIGHT_3_SEQUENCE=TCTGCCAAACATACCAACAATC
PRIMER_LEFT_3=271,22
PRIMER_RIGHT_3=480,22
PRIMER_LEFT_3_TM=60.036
PRIMER_RIGHT_3_TM=59.867
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=210
PRIMER_PAIR_PENALTY_4=0.1690
PRIMER_LEFT_4_PENALTY=0.036173
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_LEFT_4_SEQUENCE=AGTCTCAGTGACCACAATCCCT
PRIMER_RIGHT_4_SEQUENCE=TGATTCTGCCAAACATACCAAC
PRIMER_LEFT_4=271,22
PRIMER_RIGHT_4=484,22
PRIMER_LEFT_4_TM=60.036
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_05768 Ps_contig_mira-and-tgicl-ass_franssen_39857
SEQUENCE=TTCGGGGGAGAAGAGAAAGAGGGAAAAGGGAATTGAGCAGAAAACAGGGTTTGTACAGAGTAGCCAGCAACGACGATTTTTTTTGAAGAGTGAAGAAGAAAAGCAGATGGGTAAAGGTAATCCACAGAAATCAGGAACCGGCGGGGATCTTCACGCGGCGGCGAGGTCCGGCGATCTCACAGCAGTTCAGTCAATTTTGATTTCAAATCCTTTGTCCGTTAATTCCAGAGATAAGCATTCCAGAACACCACTTCATTTAGCAGCATTTTCTGGGCAAACTGAGGTAGTCAGTTACCTATGCAAGAACAAGGCTGATGTTGGTGCTTCCGCTATGGATGACATGGCTGCAATACACTTTGCGGCACAAAAGGGTCACTTAGAAGTTGTTCGGGCTCTGGTTTCGGCTGGTGCCTCTTTCAAAGCTGCTACTCGTAAAGGCATGACTCCATTACACTTTGCTGCTCAAGGCTCCCATTTGGAACTTGTCAAGTATTTGGCTAAGAAAGGGGCAAGTCTAAGTGTCAAGACAAAGGCGGGAAAGACTCCGTTGGATCTTGCTACCAATGATGAAGTCCGCACCTTTCTGGAGGGATTTGTAAAATCAGATAAGAATGAAGAATCGAGAAACGGAGATAAAGCTGAAGAATCTGATCCAAAGCAGAATGAAGGATCCAATCCAAAGGCAGACGAAGAATCTGATCCAAAAGAAAAGGAAGAATCCAATCGAAAGGCAGCTGAAGAATCTGATCCAAAAGAAAAGGAAGAATCCAATCGAAAGGCAGCTGAAGAATCTGACTCAAAAGCATCCTCCTTGGGATTCAAAGGTGATGAACCTCCTACAGTTGCTGTTGATGAAGAAGAGGGAGAAAGAGGAAAGAGGAAGGTTAATGAAAATGATGCAACAGAAGACTCATCGAAAACCAAAAAAGGCAAGAGTTAAGCTAAGCCACCTTCAAAGCTCAGATGATGCTGAAGAAGAAGAAAACTGAAAACTTGTAGGTTAGAGGCATAATAGAATGGAAATTTTGTTCTCATAGCTTGTAATAGTATCTGATTTATAATTTGCAATCCCTTTGTTGGGTTGGTAGGTTGGACCATCTATTG
TARGET=972,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1526
PRIMER_LEFT_PENALTY=0.043121
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=TAAGCTAAGCCACCTTCAAAGC
PRIMER_RIGHT_SEQUENCE=TAGATGGTCCAACCTACCAACC
PRIMER_LEFT=937,22
PRIMER_RIGHT=1100,22
PRIMER_LEFT_TM=60.043
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.2276
PRIMER_LEFT_1_PENALTY=0.118120
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=ATGCAACAGAAGACTCATCGAA
PRIMER_RIGHT_1_SEQUENCE=TAGATGGTCCAACCTACCAACC
PRIMER_LEFT_1=896,22
PRIMER_RIGHT_1=1100,22
PRIMER_LEFT_1_TM=59.882
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.2767
PRIMER_LEFT_2_PENALTY=0.167146
PRIMER_RIGHT_2_PENALTY=0.109510
PRIMER_LEFT_2_SEQUENCE=AGGGAGAAAGAGGAAAGAGGAA
PRIMER_RIGHT_2_SEQUENCE=TAGATGGTCCAACCTACCAACC
PRIMER_LEFT_2=860,22
PRIMER_RIGHT_2=1100,22
PRIMER_LEFT_2_TM=59.833
PRIMER_RIGHT_2_TM=60.110
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=0.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=0.2767
PRIMER_LEFT_3_PENALTY=0.167146
PRIMER_RIGHT_3_PENALTY=0.109510
PRIMER_LEFT_3_SEQUENCE=GGGAGAAAGAGGAAAGAGGAAG
PRIMER_RIGHT_3_SEQUENCE=TAGATGGTCCAACCTACCAACC
PRIMER_LEFT_3=861,22
PRIMER_RIGHT_3=1100,22
PRIMER_LEFT_3_TM=59.833
PRIMER_RIGHT_3_TM=60.110
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=0.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=0.2767
PRIMER_LEFT_4_PENALTY=0.167146
PRIMER_RIGHT_4_PENALTY=0.109510
PRIMER_LEFT_4_SEQUENCE=GGAGAAAGAGGAAAGAGGAAGG
PRIMER_RIGHT_4_SEQUENCE=TAGATGGTCCAACCTACCAACC
PRIMER_LEFT_4=862,22
PRIMER_RIGHT_4=1100,22
PRIMER_LEFT_4_TM=59.833
PRIMER_RIGHT_4_TM=60.110
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=0.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_05769 Ps_contig_mira-and-tgicl-ass_franssen_39858
SEQUENCE=TAAAATAGGTTTTGGGAAAAGTTTTAAGGTTTGGGTTGGGGGCGTGAGGTAAAAAAATTTTAATTTTTTNTAAGGATAAGAAACATTAGATTTTATTATCGTTTTCTTGCTTTAGTCAACACCAAAGTAGATATAAAAGTGTTATCTCAGCGGCTTGGTTTAGGTAAAGGAGGTCTCAGAATGGCACCTGCAGAGGCTTTAACAGAAATGCTTCAGGTGCCCTTGGGTTGTGTCACCCCGTTTGCGGTAGTGAATGACTCAGCACGAGATGTTGTGCTCTTGTTGGATCAAGGGTTCAAGACTCATGAGTTCTGCTTCTTCCATCCACTGGCAAATGACATGACTATATCTTTAAATGCTCATGACCTTGACAAATTTCTAAAGTCAATAGGAAAGGATCCCTCGTATGTTGATTTTGAGGCCATTCCTGTAGTGAGCAAAGATCAACCTCCTGATCTAGCTGCACTAGTTCCATCCAGCTCAATAGTTGTGCCACACCAGTCCGAAATGCAACCTTCATCACAAGTTCCTGCAGATGGAAACCATGCTTCTGTTGAGAATAAGTCCCAGATAGTTTCAGAACAAGTTTTCAAACCATCCATTAGTAAGAAAGTTACAAAGGAGAAACCAGTCAACAATGGTCATTCATCAAACTCCTTTGCAGATGTTGGGCCATTAGTTGAAGAAATATTGCATAAAGCATCAGATTTACTGCTTTCAGAGATCAAGGAAGACACTATAAAACAACATGGAGAGCATCTAGGCACTGTAGTGTCTGACAATCTACGGAAAGTTCTCGTCTCAGATTTGAAAAACCTTGCTATGATATTTAAGAACTCAGCATACACAGAAGGGTTCCATGCTGGTATTCACCATCAGTCCAAACATCTGTGAAGATCATTTCAATTATTTGAGAGACTGAAATTTCAATTCTAATTGTGCCATAATAAAGGAGATATTGTGTGGCCCAGAAGGGGATATAAGTTGTTACAAATTGGTTCATGAATTGTGTGTGTGTAAAATATGTGGTAAACTTTGTCCTATAATACATCATTTCGNTGAAAGATATTTTCTTCTGTTATAAGGTAATGTTTCTCAGTCGGAACACAGCTTTTCTAGATAGGCCAAATGTTGTTGAAATACTACACCCGTGTGTGTATATACTTATTGTTGAGAAAACAAGTGTGTGTTGACAGTGTTGAGAAACG
TARGET=1008,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4694
PRIMER_LEFT_PENALTY=0.108206
PRIMER_RIGHT_PENALTY=0.361206
PRIMER_LEFT_SEQUENCE=AGCATACACAGAAGGGTTCCAT
PRIMER_RIGHT_SEQUENCE=TGTGTTCCGACTGAGAAACATT
PRIMER_LEFT=839,22
PRIMER_RIGHT=1108,22
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=59.639
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=270
PRIMER_PAIR_PENALTY_1=0.5085
PRIMER_LEFT_1_PENALTY=0.147295
PRIMER_RIGHT_1_PENALTY=0.361206
PRIMER_LEFT_1_SEQUENCE=CTGGTATTCACCATCAGTCCAA
PRIMER_RIGHT_1_SEQUENCE=TGTGTTCCGACTGAGAAACATT
PRIMER_LEFT_1=862,22
PRIMER_RIGHT_1=1108,22
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=59.639
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.5511
PRIMER_LEFT_2_PENALTY=0.108206
PRIMER_RIGHT_2_PENALTY=0.442900
PRIMER_LEFT_2_SEQUENCE=AGCATACACAGAAGGGTTCCAT
PRIMER_RIGHT_2_SEQUENCE=TAGAAAAGCTGTGTTCCGACTG
PRIMER_LEFT_2=839,22
PRIMER_RIGHT_2=1117,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=59.557
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.5652
PRIMER_LEFT_3_PENALTY=0.203971
PRIMER_RIGHT_3_PENALTY=0.361206
PRIMER_LEFT_3_SEQUENCE=TAAAGGAGATATTGTGTGGCCC
PRIMER_RIGHT_3_SEQUENCE=TGTGTTCCGACTGAGAAACATT
PRIMER_LEFT_3=947,22
PRIMER_RIGHT_3=1108,22
PRIMER_LEFT_3_TM=60.204
PRIMER_RIGHT_3_TM=59.639
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=12.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=0.5886
PRIMER_LEFT_4_PENALTY=0.227356
PRIMER_RIGHT_4_PENALTY=0.361206
PRIMER_LEFT_4_SEQUENCE=TGGTATTCACCATCAGTCCAAA
PRIMER_RIGHT_4_SEQUENCE=TGTGTTCCGACTGAGAAACATT
PRIMER_LEFT_4=863,22
PRIMER_RIGHT_4=1108,22
PRIMER_LEFT_4_TM=60.227
PRIMER_RIGHT_4_TM=59.639
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_05770 Ps_contig_mira-and-tgicl-ass_franssen_39860
SEQUENCE=GYATTCCACCAACCTGCGAACCATCCCCTAATATCTCACTTCATTCTTCCACAAATAAACCGGTGAACATTTAAGTGACTCCGCCGCGCCGGAACTCATCGTCGGCGGCGGCGGAGCTCCTAAGCCTCAAACTCACGCCGCTCTCACCATCCCCAAACTCTCGATTCTGAATCCTCAACTAAACTCTCTTAATCCCTACCTAAACAAAGTGAACTGAATCGTGATCTCTCACGAGCTTCAATCAAAATTCAGAATCCGATCCTCTCACGGAGGAATCTAAGATCTCAAAACCGACTCGACAATGACGAAACCCAGTGAATCGACGGATTACCTAATCGTGAAGGTGACCGTGTGGAACTAACGAGACGGATGAATGTGAACTCAAGTGTGCAGCTCCTTCGGAATCTCTTCTATACTCTCTTCTGTCCGGCGGTGATGATCGAAGATCCGCGGTTCAATTCGCAAGTATTGTTGAGGTGCGGCGAGAGTTATGGGAAGTGCGGCCGAGATTCGATTCAACCGAGAGGATTATGCAGGCGGCGGTGAATGTGGCGCGAGAATAARAAAATAGGATTTCTG
TARGET=103,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05771 Ps_contig_mira-and-tgicl-ass_franssen_39874
SEQUENCE=TTAGTTYTAGNGTTTAACTAGCATTCTGAATATATTCGACANTNAAAAAAAAGAAGAAATATTCGACATGAGATGAAAGAATATAGTTATGATTCTTGTCATGCCCATTTCAAAATATATACAAAACTATGTAAAAGAACAAAAAGTGAGACATAGATAAACACTATCACATTACGTTAAAGAGAAAAGGAAAAAAGAATAAAGACAACACAGCTTAATTCTAGCCCATTAAGGTTACGTACACACACACACCTTAACATATAATAATAACATTTAACAAACAAAAAAACCGGTTCAATATATATGATAGTACTATAAACTAGGGAACTGAACCGGTTTGGTTCAAGCACCCGGCGCTGCATCCTTGACCTTACTCTGCAAATACCCACCGTATTCTATAGCACAATCTTTAACATGGGTGGCCGTATCATAAATCCGGCTCCGGGCGTAATCGACCCGGTCCGAACCGGGCGGGTGGAGACCCCTAAAGTAACGGTAGGACCAATGGGAAACAGCGACGGCGATGACACCAAATCCACATATGGACAAGAATCCAGCGACGAGGAGGAAGAAGAAGGTGCCGGCTGGGACCCATATCGGGCTTGAAACGATGATCAACGGCGAGAAGAAGATGAGRGCGAAAACAGTACCAGAGACGGTTAAACCGGTTAGAAGGAGGAAAATGGAACCGGTAGCGAGGAGGGTTAAGATACCAGCGAGCTGGGTTGAGTTTGATGCCTGGAGGTTGTCTTGAAGCTTTCTTAGAAACACGGCGGATGAGGAGGACGTGGCGGTGGCGGGTCTATGTGTTTGGTTAAGATCCGCCATTGGGAATATGGTTTGTTTTGTTGTCCCCCCTACTCTGCGTTGATACCA
TARGET=241,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05772 Ps_contig_mira-and-tgicl-ass_franssen_39875
SEQUENCE=CTTTTTTTTACTTCTAATAAGGCTTTCATTTAGATTTAAATAAACTATACACAACATATATATGCAAAATGCAGAAGACACATGTAAAGAACTGTGTCAACGATGACTGCAAAATGGATAACAACTTCACCCTGGATGATATTTTACAAATGAACTAAACCAACAAAGCCAAGTAAACTGAAGTTCAATATATGTGACTACTAGGTTTCTAATAGTAAAGTACGTGTAATAATGTTGCCTTTAATTTAGCCGAGGCGCGATTTGAGATATTCTTTCATCTTCTAAGAGTGCGTAAGGGTCCACGTATTGTGGAGTTGATAGAGACATCTTGCTTCCGTCGTGAACAAAACTCGGTCCAAAATAGAAGTAAAGAGCAGAAAATAATGCCACGGTTATGAGGATCAACGGTCCAAATGCTAAGAAAAATGTTCCTCCAAGTGTCAATAGAAGTTTAGCTCGAGGCACCAAGCCCTCAAGACCTTCACTGTTCAGCGCCATAGACCGCTCCCTTTGATTTTCTAGACCTCTCTCAATATTGGTTGACACTGCTTTTCTCTTTGGGAAACTTTTATCTTCTTCATTCACAAAAGCCTCTTCATCAAGTCCATAGTTGGGTTGCTTGTAATCCTCAAGCAATGATGTAGGATTCATACCTCTCCTCTTCAATTCCTTCATAAATAATGACTCCGGAGGCTCTTCTCCATTGTTTTGCTTTTGAGTATCTTTAGAACTGGAGCAGACCCTGAAAGAAGTGGAAGATTTAGAAGTGTTGAAATTTTGCATCCTTTTGTGGAAAAAACAGTGTTGAGGATTATTCCATGTAGAATTTCTGGGAAAATTAGATTGGGAAAGGGAAATTTGAGAGAGGGTAGGTCTCATATACGCCATAACTAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGTGGTGAAGAGTAGTTCTACGTAACGTTTTCGTTACCTTTCCCGTCCGTTACCCGTCCGTCGTCGTTGTACGTACGACTACCGTCCTTCGTCGTACGTACGAACGGACC
TARGET=894,32
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1550
PRIMER_LEFT_PENALTY=0.018946
PRIMER_RIGHT_PENALTY=1.136051
PRIMER_LEFT_SEQUENCE=GGAAAATTAGATTGGGAAAGGG
PRIMER_RIGHT_SEQUENCE=GACGGGAAAGGTAACGAAAAC
PRIMER_LEFT=832,22
PRIMER_RIGHT=971,21
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=59.864
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=140
PRIMER_PAIR_PENALTY_1=1.1550
PRIMER_LEFT_1_PENALTY=0.018946
PRIMER_RIGHT_1_PENALTY=1.136051
PRIMER_LEFT_1_SEQUENCE=GGGAAAATTAGATTGGGAAAGG
PRIMER_RIGHT_1_SEQUENCE=GACGGGAAAGGTAACGAAAAC
PRIMER_LEFT_1=831,22
PRIMER_RIGHT_1=971,21
PRIMER_LEFT_1_TM=60.019
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=1.2505
PRIMER_LEFT_2_PENALTY=0.114470
PRIMER_RIGHT_2_PENALTY=1.136051
PRIMER_LEFT_2_SEQUENCE=TCTTTAGAACTGGAGCAGACCC
PRIMER_RIGHT_2_SEQUENCE=GACGGGAAAGGTAACGAAAAC
PRIMER_LEFT_2=721,22
PRIMER_RIGHT_2=971,21
PRIMER_LEFT_2_TM=59.886
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=1.2586
PRIMER_LEFT_3_PENALTY=0.122569
PRIMER_RIGHT_3_PENALTY=1.136051
PRIMER_LEFT_3_SEQUENCE=AGGGTAGGTCTCATATACGCCA
PRIMER_RIGHT_3_SEQUENCE=GACGGGAAAGGTAACGAAAAC
PRIMER_LEFT_3=865,22
PRIMER_RIGHT_3=971,21
PRIMER_LEFT_3_TM=59.877
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=1.2644
PRIMER_LEFT_4_PENALTY=0.018946
PRIMER_RIGHT_4_PENALTY=1.245492
PRIMER_LEFT_4_SEQUENCE=GGAAAATTAGATTGGGAAAGGG
PRIMER_RIGHT_4_SEQUENCE=GTAACGGACGGGAAAGGTAAC
PRIMER_LEFT_4=832,22
PRIMER_RIGHT_4=977,21
PRIMER_LEFT_4_TM=60.019
PRIMER_RIGHT_4_TM=59.755
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=146
=
PRIMER_SEQUENCE_ID=SSR_05773 Ps_contig_mira-and-tgicl-ass_franssen_39876
SEQUENCE=CGGGGTATTTCTATTTTCCTATTTACTTCTCCCCAAACCCTAAACGAAAATCAATCGCTACTCTCTCTCTCTTATGGCTACCATTAACGTCCACACTGAAGCTGGTCAAGACAACATTGCTCCCTCAAAGCAATCTCTTCCTCCGCCTAAATCCGTTGATTCTCAATCTGTTCTCAGAAGGTTGCAGTCTGAATTGATGGCTTTGATGATGGGTGGAGATTCTGGTATATCTGCGTTCCCTGAGGAGGATAATATATTGTGTTGGAAAGGAACAATAACAGGAAGCAAAGAAACTGTATTTGAGGGAACAGAATATAAACTCTCTCTTTCATTTCCCAATGAGTATCCTTTTAAGGCTCCCAAGGTCAAATTTGATACCACCTGCTTCCACCCCAATGTAGATTTGCATGGCAATATTTGCTTAGATATTCTTCAGGATAAATGGTCATCTGCCTATGATGTAAGAACCATTCTTCTATCAATTCAAAGCCTGCTAGGAGAGCCAAACATTAGCTCGCCGTTAAATCCACAAGCTGCACAGCTTTGGAGCAATCAAGAAGAATACAGGAAGATGGTGGAGAAGTACAAGTCTCCTGCGGCTTAACTTTGATCTCAGAGTTCATTGATTTATGATTCTCTCTGTCTGGCTGGTTTGCTTTGGAGTTTGGAAAAGAGAAGGGATGAAATTTTTAAAGCTGTTCCATCCTTTCCTTTTGGACTTTCAAATGAGGCTTCTAAATTTTCTATACATGAATGATTGTTGTATCTCTCTTTTGTTGACATTTTTAGTTAAAAGCAAAACATTGTTGCAGATTAGTTTATTCTTTTTGGATCTTTGGAGAGGGTGCAATGTAATGTACAAGATTATCAGTTTCAACAATGGAATTTGGTTTACCTTTT
TARGET=61,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9811
PRIMER_LEFT_PENALTY=0.967677
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=TTACTTCTCCCCAAACCCTAAA
PRIMER_RIGHT_SEQUENCE=AGGGAACGCAGATATACCAGAA
PRIMER_LEFT=22,22
PRIMER_RIGHT=240,22
PRIMER_LEFT_TM=59.032
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.9811
PRIMER_LEFT_1_PENALTY=0.967677
PRIMER_RIGHT_1_PENALTY=0.013401
PRIMER_LEFT_1_SEQUENCE=TTTACTTCTCCCCAAACCCTAA
PRIMER_RIGHT_1_SEQUENCE=AGGGAACGCAGATATACCAGAA
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=240,22
PRIMER_LEFT_1_TM=59.032
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=220
PRIMER_PAIR_PENALTY_2=1.0302
PRIMER_LEFT_2_PENALTY=0.967677
PRIMER_RIGHT_2_PENALTY=0.062568
PRIMER_LEFT_2_SEQUENCE=TTACTTCTCCCCAAACCCTAAA
PRIMER_RIGHT_2_SEQUENCE=GATTTAGGCGGAGGAAGAGATT
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=152,22
PRIMER_LEFT_2_TM=59.032
PRIMER_RIGHT_2_TM=60.063
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=131
PRIMER_PAIR_PENALTY_3=1.0302
PRIMER_LEFT_3_PENALTY=0.967677
PRIMER_RIGHT_3_PENALTY=0.062568
PRIMER_LEFT_3_SEQUENCE=TTTACTTCTCCCCAAACCCTAA
PRIMER_RIGHT_3_SEQUENCE=GATTTAGGCGGAGGAAGAGATT
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=152,22
PRIMER_LEFT_3_TM=59.032
PRIMER_RIGHT_3_TM=60.063
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=1.0540
PRIMER_LEFT_4_PENALTY=0.967677
PRIMER_RIGHT_4_PENALTY=0.086339
PRIMER_LEFT_4_SEQUENCE=TTACTTCTCCCCAAACCCTAAA
PRIMER_RIGHT_4_SEQUENCE=CAATTCAGACTGCAACCTTCTG
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=195,22
PRIMER_LEFT_4_TM=59.032
PRIMER_RIGHT_4_TM=59.914
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=174
=
PRIMER_SEQUENCE_ID=SSR_05774 Ps_contig_mira-and-tgicl-ass_franssen_39878
SEQUENCE=TCGCTTCACAATCAGGGCCAGAGATTCCAAAGAAATCTTATCAATGCTCAGGCTCAGTAGGTTTGACTTCATCAGCTTTTATGCTGTCCTCACCTCCATCCTCGGGCAAATCAGAGGTCCAGAGGGTCTCTCTCTCTTTCTCGGCGGACATTGTGTCGTTTCAGAGAGAGATCGCAAAAAGTGAGAGAAAGCTGAAAGGGGGGACGGAGGAAAGGAAGGAAACAAATAAAGAAAATAGAGATGATGCGACGACCCCG
TARGET=127,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1581
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=CTTTTATGCTGTCCTCACCTCC
PRIMER_RIGHT_SEQUENCE=GCTTTCTCTCACTTTTTGCGAT
PRIMER_LEFT=75,22
PRIMER_RIGHT=191,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=9.8000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=117
PRIMER_PAIR_PENALTY_1=0.2973
PRIMER_LEFT_1_PENALTY=0.269585
PRIMER_RIGHT_1_PENALTY=0.027715
PRIMER_LEFT_1_SEQUENCE=GCTTTTATGCTGTCCTCACCTC
PRIMER_RIGHT_1_SEQUENCE=GCTTTCTCTCACTTTTTGCGAT
PRIMER_LEFT_1=74,22
PRIMER_RIGHT_1=191,22
PRIMER_LEFT_1_TM=60.270
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=0.3039
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.173582
PRIMER_LEFT_2_SEQUENCE=CTTTTATGCTGTCCTCACCTCC
PRIMER_RIGHT_2_SEQUENCE=ATTTGTTTCCTTCCTTTCCTCC
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=226,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=59.826
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.4432
PRIMER_LEFT_3_PENALTY=0.269585
PRIMER_RIGHT_3_PENALTY=0.173582
PRIMER_LEFT_3_SEQUENCE=GCTTTTATGCTGTCCTCACCTC
PRIMER_RIGHT_3_SEQUENCE=ATTTGTTTCCTTCCTTTCCTCC
PRIMER_LEFT_3=74,22
PRIMER_RIGHT_3=226,22
PRIMER_LEFT_3_TM=60.270
PRIMER_RIGHT_3_TM=59.826
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=153
PRIMER_PAIR_PENALTY_4=0.4718
PRIMER_LEFT_4_PENALTY=0.444124
PRIMER_RIGHT_4_PENALTY=0.027715
PRIMER_LEFT_4_SEQUENCE=GCTCAGGCTCAGTAGGTTTGAC
PRIMER_RIGHT_4_SEQUENCE=GCTTTCTCTCACTTTTTGCGAT
PRIMER_LEFT_4=45,22
PRIMER_RIGHT_4=191,22
PRIMER_LEFT_4_TM=60.444
PRIMER_RIGHT_4_TM=60.028
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_05775 Ps_contig_mira-and-tgicl-ass_franssen_39884
SEQUENCE=CTTTCTTCTAATCTCTAATACTACAAAGAAAACCTAAAACCTAACTAAAGCATAAAAAGACTAANCCTTGTCCCCTTTTTTACTTATTCATTTTTTTCTTCCTTAAATTATTATTAATATCACAAAAATCTTTTCTTCAACCATAGATGTTAGAGCCATGGACATAACCCCAACACAAACAACAACAACAACACCATTACCAACCATTTTAACAACATCTACAACAACCTTAACAAAATCACCAGAACATGAAACCGAAACACCCACCAAAATCACCACTCCCACTACAACCACAACACCAAAAATCTCATCTTTTTCCAACGGTGTTCTCAAACGCCTCCACAACCACCACCACCACCACCTTAACAACCACCACAACAACCTCGTAACTGTTACTTACAAAGAATGTCTCAAAAACCACGCAGCAAACTTAGGTGGTCACGCCCTCGACGGCTGCGGCGAATTCATGACATCCCCCACCGCAACATCCGCCGACCCAACATCCTTAAAATGCGCTGCCTGTGGCTGTCACCGTAATTTCCACCGCCGTGAACCCGAAGAGCCACATCTCTCCACCACCACGCATGTAATCGAATATCAACCTCACCACCGTCATCAGCCTCTCCCACCGCCGTTCTCCACCCGCAGCCCGAATTCATCCTCTCCACCGCCGATCTCCTCAACTTACCTACCCCTCCGCCCCTCACATGCTCCTCGCCCTCTCCACCGCCTTGCCGGAAAACGTCTCAGCCCCTAACTCCGCCATGATCTCTCCTGGCTCCCACACTAGAAAACGCTTCCGAACAAAATTCACTCAAGATCAGAAAGAAAAGATGCTGAAATTCGCTGAGAAAGTTGGTTGGAAGATGCAGAAAAAAGACGAAGATTATGTTCATGAATTCTGCAACGAAATCTGTGTTGATCGAAGTGTTCTCAAAGTTTGGATGCACAACAACAAAAACACTTTCGCTAAAAAAGACAACAACATCAACAACCTCAACGACATCGACAACAACCTCAACAACATCAACAATGAGGTTAATGTTGGTGTTAAGGTAGTTTCTTGTCCTTCGTTTAGAAGTAATAGAAAGAAACAACAAACAATACTAAA
TARGET=178,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3778
PRIMER_LEFT_PENALTY=0.269153
PRIMER_RIGHT_PENALTY=0.108684
PRIMER_LEFT_SEQUENCE=GTTAGAGCCATGGACATAACCC
PRIMER_RIGHT_SEQUENCE=TGTAGTGGGAGTGGTGATTTTG
PRIMER_LEFT=148,22
PRIMER_RIGHT=288,22
PRIMER_LEFT_TM=59.731
PRIMER_RIGHT_TM=59.891
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.4223
PRIMER_LEFT_1_PENALTY=0.269153
PRIMER_RIGHT_1_PENALTY=0.153161
PRIMER_LEFT_1_SEQUENCE=GTTAGAGCCATGGACATAACCC
PRIMER_RIGHT_1_SEQUENCE=GGTTGTAGTGGGAGTGGTGATT
PRIMER_LEFT_1=148,22
PRIMER_RIGHT_1=291,22
PRIMER_LEFT_1_TM=59.731
PRIMER_RIGHT_1_TM=60.153
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=144
PRIMER_PAIR_PENALTY_2=0.4725
PRIMER_LEFT_2_PENALTY=0.269153
PRIMER_RIGHT_2_PENALTY=0.203324
PRIMER_LEFT_2_SEQUENCE=GTTAGAGCCATGGACATAACCC
PRIMER_RIGHT_2_SEQUENCE=GAGGTTGTTGTGGTGGTTGTTA
PRIMER_LEFT_2=148,22
PRIMER_RIGHT_2=384,22
PRIMER_LEFT_2_TM=59.731
PRIMER_RIGHT_2_TM=59.797
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=0.6215
PRIMER_LEFT_3_PENALTY=0.269153
PRIMER_RIGHT_3_PENALTY=0.352395
PRIMER_LEFT_3_SEQUENCE=GTTAGAGCCATGGACATAACCC
PRIMER_RIGHT_3_SEQUENCE=CACCGTTGGAAAAAGATGAGAT
PRIMER_LEFT_3=148,22
PRIMER_RIGHT_3=325,22
PRIMER_LEFT_3_TM=59.731
PRIMER_RIGHT_3_TM=60.352
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=178
PRIMER_PAIR_PENALTY_4=0.6538
PRIMER_LEFT_4_PENALTY=0.269153
PRIMER_RIGHT_4_PENALTY=0.384647
PRIMER_LEFT_4_SEQUENCE=GTTAGAGCCATGGACATAACCC
PRIMER_RIGHT_4_SEQUENCE=AACACCGTTGGAAAAAGATGAG
PRIMER_LEFT_4=148,22
PRIMER_RIGHT_4=327,22
PRIMER_LEFT_4_TM=59.731
PRIMER_RIGHT_4_TM=60.385
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=180
=
PRIMER_SEQUENCE_ID=SSR_05776 Ps_contig_mira-and-tgicl-ass_franssen_39884
SEQUENCE=CTTTCTTCTAATCTCTAATACTACAAAGAAAACCTAAAACCTAACTAAAGCATAAAAAGACTAANCCTTGTCCCCTTTTTTACTTATTCATTTTTTTCTTCCTTAAATTATTATTAATATCACAAAAATCTTTTCTTCAACCATAGATGTTAGAGCCATGGACATAACCCCAACACAAACAACAACAACAACACCATTACCAACCATTTTAACAACATCTACAACAACCTTAACAAAATCACCAGAACATGAAACCGAAACACCCACCAAAATCACCACTCCCACTACAACCACAACACCAAAAATCTCATCTTTTTCCAACGGTGTTCTCAAACGCCTCCACAACCACCACCACCACCACCTTAACAACCACCACAACAACCTCGTAACTGTTACTTACAAAGAATGTCTCAAAAACCACGCAGCAAACTTAGGTGGTCACGCCCTCGACGGCTGCGGCGAATTCATGACATCCCCCACCGCAACATCCGCCGACCCAACATCCTTAAAATGCGCTGCCTGTGGCTGTCACCGTAATTTCCACCGCCGTGAACCCGAAGAGCCACATCTCTCCACCACCACGCATGTAATCGAATATCAACCTCACCACCGTCATCAGCCTCTCCCACCGCCGTTCTCCACCCGCAGCCCGAATTCATCCTCTCCACCGCCGATCTCCTCAACTTACCTACCCCTCCGCCCCTCACATGCTCCTCGCCCTCTCCACCGCCTTGCCGGAAAACGTCTCAGCCCCTAACTCCGCCATGATCTCTCCTGGCTCCCACACTAGAAAACGCTTCCGAACAAAATTCACTCAAGATCAGAAAGAAAAGATGCTGAAATTCGCTGAGAAAGTTGGTTGGAAGATGCAGAAAAAAGACGAAGATTATGTTCATGAATTCTGCAACGAAATCTGTGTTGATCGAAGTGTTCTCAAAGTTTGGATGCACAACAACAAAAACACTTTCGCTAAAAAAGACAACAACATCAACAACCTCAACGACATCGACAACAACCTCAACAACATCAACAATGAGGTTAATGTTGGTGTTAAGGTAGTTTCTTGTCCTTCGTTTAGAAGTAATAGAAAGAAACAACAAACAATACTAAA
TARGET=345,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4725
PRIMER_LEFT_PENALTY=0.269153
PRIMER_RIGHT_PENALTY=0.203324
PRIMER_LEFT_SEQUENCE=GTTAGAGCCATGGACATAACCC
PRIMER_RIGHT_SEQUENCE=GAGGTTGTTGTGGTGGTTGTTA
PRIMER_LEFT=148,22
PRIMER_RIGHT=384,22
PRIMER_LEFT_TM=59.731
PRIMER_RIGHT_TM=59.797
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.5744
PRIMER_LEFT_1_PENALTY=0.052187
PRIMER_RIGHT_1_PENALTY=0.522246
PRIMER_LEFT_1_SEQUENCE=ACAACAACACCATTACCAACCA
PRIMER_RIGHT_1_SEQUENCE=GCGTGGTTTTTGAGACATTCTT
PRIMER_LEFT_1=184,22
PRIMER_RIGHT_1=422,22
PRIMER_LEFT_1_TM=60.052
PRIMER_RIGHT_1_TM=60.522
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.6309
PRIMER_LEFT_2_PENALTY=0.108684
PRIMER_RIGHT_2_PENALTY=0.522246
PRIMER_LEFT_2_SEQUENCE=CAAAATCACCACTCCCACTACA
PRIMER_RIGHT_2_SEQUENCE=GCGTGGTTTTTGAGACATTCTT
PRIMER_LEFT_2=267,22
PRIMER_RIGHT_2=422,22
PRIMER_LEFT_2_TM=59.891
PRIMER_RIGHT_2_TM=60.522
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.6369
PRIMER_LEFT_3_PENALTY=0.384647
PRIMER_RIGHT_3_PENALTY=0.252253
PRIMER_LEFT_3_SEQUENCE=CATCTTTTTCCAACGGTGTTCT
PRIMER_RIGHT_3_SEQUENCE=ATATTCGATTACATGCGTGGTG
PRIMER_LEFT_3=308,22
PRIMER_RIGHT_3=597,22
PRIMER_LEFT_3_TM=60.385
PRIMER_RIGHT_3_TM=59.748
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=290
PRIMER_PAIR_PENALTY_4=0.6369
PRIMER_LEFT_4_PENALTY=0.384647
PRIMER_RIGHT_4_PENALTY=0.252253
PRIMER_LEFT_4_SEQUENCE=CTCATCTTTTTCCAACGGTGTT
PRIMER_RIGHT_4_SEQUENCE=ATATTCGATTACATGCGTGGTG
PRIMER_LEFT_4=306,22
PRIMER_RIGHT_4=597,22
PRIMER_LEFT_4_TM=60.385
PRIMER_RIGHT_4_TM=59.748
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=292
=
PRIMER_SEQUENCE_ID=SSR_05777 Ps_contig_mira-and-tgicl-ass_franssen_39886
SEQUENCE=TAAAGGGTGTTTCTCTTGAGAAAACAAGAGGGGTATTTTGTCCTTTGTCTGATGCATTTATTTGGAATTCAATGTTGTTATCGTCTTCGTGGGTATCCATTAAAATGTAATTTTGGATCATGAGATAAGAAAGTGCGTCCTGTTGTGTGAAGCTAAAGAGGAGAGAGAGAATCCATGGTGTATGATTTATAGGTTTACATCTGATTGCGAAACGAAACGAAACGAAACCCCTACTGCAGGCCTGACCTATCTATGAATTCTTATGCGACTTTCATTTCAATTTCAATTTCAATTCCAATTCCAATTCAGACGCCAAATATAGAGACAGCACCAACCAATACTACCCTATTTTAAACTCCCCACTTTATTATTAATAACATCCTCACTATTTCCCG
TARGET=161,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5105
PRIMER_LEFT_PENALTY=0.338748
PRIMER_RIGHT_PENALTY=0.171757
PRIMER_LEFT_SEQUENCE=TAAGAAAGTGCGTCCTGTTGTG
PRIMER_RIGHT_SEQUENCE=GGGTAGTATTGGTTGGTGCTGT
PRIMER_LEFT=125,22
PRIMER_RIGHT=345,22
PRIMER_LEFT_TM=60.339
PRIMER_RIGHT_TM=60.172
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=221
PRIMER_PAIR_PENALTY_1=0.5105
PRIMER_LEFT_1_PENALTY=0.338748
PRIMER_RIGHT_1_PENALTY=0.171757
PRIMER_LEFT_1_SEQUENCE=CGTCCTGTTGTGTGAAGCTAAA
PRIMER_RIGHT_1_SEQUENCE=GGGTAGTATTGGTTGGTGCTGT
PRIMER_LEFT_1=135,22
PRIMER_RIGHT_1=345,22
PRIMER_LEFT_1_TM=60.339
PRIMER_RIGHT_1_TM=60.172
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.6337
PRIMER_LEFT_2_PENALTY=0.338748
PRIMER_RIGHT_2_PENALTY=0.294975
PRIMER_LEFT_2_SEQUENCE=TAAGAAAGTGCGTCCTGTTGTG
PRIMER_RIGHT_2_SEQUENCE=AGGGTAGTATTGGTTGGTGCTG
PRIMER_LEFT_2=125,22
PRIMER_RIGHT_2=346,22
PRIMER_LEFT_2_TM=60.339
PRIMER_RIGHT_2_TM=60.295
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.6337
PRIMER_LEFT_3_PENALTY=0.338748
PRIMER_RIGHT_3_PENALTY=0.294975
PRIMER_LEFT_3_SEQUENCE=CGTCCTGTTGTGTGAAGCTAAA
PRIMER_RIGHT_3_SEQUENCE=AGGGTAGTATTGGTTGGTGCTG
PRIMER_LEFT_3=135,22
PRIMER_RIGHT_3=346,22
PRIMER_LEFT_3_TM=60.339
PRIMER_RIGHT_3_TM=60.295
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.6550
PRIMER_LEFT_4_PENALTY=0.483253
PRIMER_RIGHT_4_PENALTY=0.171757
PRIMER_LEFT_4_SEQUENCE=TGAGATAAGAAAGTGCGTCCTG
PRIMER_RIGHT_4_SEQUENCE=GGGTAGTATTGGTTGGTGCTGT
PRIMER_LEFT_4=120,22
PRIMER_RIGHT_4=345,22
PRIMER_LEFT_4_TM=59.517
PRIMER_RIGHT_4_TM=60.172
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_05778 Ps_contig_mira-and-tgicl-ass_franssen_39886
SEQUENCE=TAAAGGGTGTTTCTCTTGAGAAAACAAGAGGGGTATTTTGTCCTTTGTCTGATGCATTTATTTGGAATTCAATGTTGTTATCGTCTTCGTGGGTATCCATTAAAATGTAATTTTGGATCATGAGATAAGAAAGTGCGTCCTGTTGTGTGAAGCTAAAGAGGAGAGAGAGAATCCATGGTGTATGATTTATAGGTTTACATCTGATTGCGAAACGAAACGAAACGAAACCCCTACTGCAGGCCTGACCTATCTATGAATTCTTATGCGACTTTCATTTCAATTTCAATTTCAATTCCAATTCCAATTCAGACGCCAAATATAGAGACAGCACCAACCAATACTACCCTATTTTAAACTCCCCACTTTATTATTAATAACATCCTCACTATTTCCCG
TARGET=208,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4723
PRIMER_LEFT_PENALTY=0.300539
PRIMER_RIGHT_PENALTY=0.171757
PRIMER_LEFT_SEQUENCE=AGGAGAGAGAGAATCCATGGTG
PRIMER_RIGHT_SEQUENCE=GGGTAGTATTGGTTGGTGCTGT
PRIMER_LEFT=158,22
PRIMER_RIGHT=345,22
PRIMER_LEFT_TM=59.699
PRIMER_RIGHT_TM=60.172
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=0.5105
PRIMER_LEFT_1_PENALTY=0.338748
PRIMER_RIGHT_1_PENALTY=0.171757
PRIMER_LEFT_1_SEQUENCE=TAAGAAAGTGCGTCCTGTTGTG
PRIMER_RIGHT_1_SEQUENCE=GGGTAGTATTGGTTGGTGCTGT
PRIMER_LEFT_1=125,22
PRIMER_RIGHT_1=345,22
PRIMER_LEFT_1_TM=60.339
PRIMER_RIGHT_1_TM=60.172
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.5105
PRIMER_LEFT_2_PENALTY=0.338748
PRIMER_RIGHT_2_PENALTY=0.171757
PRIMER_LEFT_2_SEQUENCE=CGTCCTGTTGTGTGAAGCTAAA
PRIMER_RIGHT_2_SEQUENCE=GGGTAGTATTGGTTGGTGCTGT
PRIMER_LEFT_2=135,22
PRIMER_RIGHT_2=345,22
PRIMER_LEFT_2_TM=60.339
PRIMER_RIGHT_2_TM=60.172
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.5955
PRIMER_LEFT_3_PENALTY=0.300539
PRIMER_RIGHT_3_PENALTY=0.294975
PRIMER_LEFT_3_SEQUENCE=AGGAGAGAGAGAATCCATGGTG
PRIMER_RIGHT_3_SEQUENCE=AGGGTAGTATTGGTTGGTGCTG
PRIMER_LEFT_3=158,22
PRIMER_RIGHT_3=346,22
PRIMER_LEFT_3_TM=59.699
PRIMER_RIGHT_3_TM=60.295
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.6337
PRIMER_LEFT_4_PENALTY=0.338748
PRIMER_RIGHT_4_PENALTY=0.294975
PRIMER_LEFT_4_SEQUENCE=TAAGAAAGTGCGTCCTGTTGTG
PRIMER_RIGHT_4_SEQUENCE=AGGGTAGTATTGGTTGGTGCTG
PRIMER_LEFT_4=125,22
PRIMER_RIGHT_4=346,22
PRIMER_LEFT_4_TM=60.339
PRIMER_RIGHT_4_TM=60.295
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=222
=
PRIMER_SEQUENCE_ID=SSR_05779 Ps_contig_mira-and-tgicl-ass_franssen_39892
SEQUENCE=ACTATTACCCTATCCCATTCAAAGTTCAAATGCAAATACAACTGGGATTGGATCAGTGCCATAATAAAGACTGATTCAATAGCATTTTATCTCTTGGACGATCTTGGTACAACATTAAGCTCACTCAAATGAGGTCTCTTTGTTTCTTCTTCTTGTCTTGGTACCTCCTCCTCCTTTGATACAGGTATGACCTTTCTGCGTATCTGTGCTTGTGTTCTAAAATGCTCGCGATGCTGTTGCTGCATTTCCTGAAACTGTTTTATCCTTGTTGCAAAATTTATTGCATTGTGTGAGTTCCCGTTGTCCACGGCTACGTCGCCATCTTCTGTGATGTGAGATCCACCAGACATGTGATGGAGAAATTGGGTAGGGGGCCCCGGAGGATGTTTTCTTCAATTGCTCACCATTGAAAGAATACGCTGGCACTAGAACTTTTAAGCGAGTTTTCAGTATCTTGAATGCARCGCTTTGCTGAGGAAGCAACATCAGAAGACCATACAGAGCTTTAAATAACCATGTATGTTTTCCAGGTTCCAGAAGCTGCAGTCGAAGATAAGCAAAGACCGGAGTTTCCAGCAAACGAATCAATTTATCCAGCTGTACTAAGAATTTGACATTAATATCCTCCTCTACCAGAGATTGAATCACAGTACTCACATGTTGGTAAGTCTGAGCTAAGAAGCAAAGACTTATAATCGCCATAGGAGAATGACACCATGAGGCATACAAAGAAACATATAAATCCCTCCCGGCAGGATTTACCAGTGTTTGCTTCAAAAGAACTCTAATCTCCGATAGTTCTGATGATGTAAGTAAGATCAAATTCAAAGCCTGAACCATAATTGATGACCAAAAACCCAATCAGTATTCTCCTTCTAGTATTGTAAGTAA
TARGET=164,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05780 Ps_contig_mira-and-tgicl-ass_franssen_39901
SEQUENCE=AACATATCTCATTCATTCATAATTATTATTACTACTACTCAATCTCTCTCTTTTCTCTCACATATACAAAACTCTTATTACATTTATTTATATACACATAATCCATGGAAGAACAAACCATGGAAATGGAAACAGCATTTCTCACTCAAAACATGAATACTACTACTGCTACTGCGACCACTACCGCTACTACCACACATGAAGTTCAAAAGTCTCTGCATGTTCTACGGCGAAGCCGTAGACGGTGCATGGATGGCGTCGGCAAGGAGAAACTGGTTTTGCTAAAGGAAGTCGCTGTTGAGGAGAAAGAAAAGGTCGAAGATGAAGAAAGTGATGATAGAGAAGAGATAGAGAGAAAGATTAATGCATTGCAGAGGATTGTACCTAACGGTGAGTCTTATGGTGTGGACAAGCTTTTTGATGAAACTGCTGGTTATATTCTGGCTTTACAGTATCAAGTCAAAGCTTTGAAAGCTCTTACTGGTTTCTTTCAGAAGATGGAGAAAGACAAAACTAAACTCGGTGGTTAATTAATTCTCATGATTCTTCAACAACAACAACTTCATGGAATTTCTTAGGTTAGAGAGGTTCTTAATTTTTGGTTTTTTTTTCTTTCATAGACCCATTAGTTTATTTAGTATTTTTATTTGGGTTTTCTTTATTTAATTGTTGTTACTTTTGGTTTCTATTGGGTAGTCAA
TARGET=9,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05781 Ps_contig_mira-and-tgicl-ass_franssen_39901
SEQUENCE=AACATATCTCATTCATTCATAATTATTATTACTACTACTCAATCTCTCTCTTTTCTCTCACATATACAAAACTCTTATTACATTTATTTATATACACATAATCCATGGAAGAACAAACCATGGAAATGGAAACAGCATTTCTCACTCAAAACATGAATACTACTACTGCTACTGCGACCACTACCGCTACTACCACACATGAAGTTCAAAAGTCTCTGCATGTTCTACGGCGAAGCCGTAGACGGTGCATGGATGGCGTCGGCAAGGAGAAACTGGTTTTGCTAAAGGAAGTCGCTGTTGAGGAGAAAGAAAAGGTCGAAGATGAAGAAAGTGATGATAGAGAAGAGATAGAGAGAAAGATTAATGCATTGCAGAGGATTGTACCTAACGGTGAGTCTTATGGTGTGGACAAGCTTTTTGATGAAACTGCTGGTTATATTCTGGCTTTACAGTATCAAGTCAAAGCTTTGAAAGCTCTTACTGGTTTCTTTCAGAAGATGGAGAAAGACAAAACTAAACTCGGTGGTTAATTAATTCTCATGATTCTTCAACAACAACAACTTCATGGAATTTCTTAGGTTAGAGAGGTTCTTAATTTTTGGTTTTTTTTTCTTTCATAGACCCATTAGTTTATTTAGTATTTTTATTTGGGTTTTCTTTATTTAATTGTTGTTACTTTTGGTTTCTATTGGGTAGTCAA
TARGET=549,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05782 Ps_contig_mira-and-tgicl-ass_franssen_39909
SEQUENCE=AAGGTTGCTTATCTGTTCCAGAGAGGAGAAGAACTGAAGAAAGCAGAGTCACACACCACACACGCACACTGTAGTTTGTTTCATTCATTTCAGTCTTGTGTTTGAGAACTCTCACTCCTTCATAATGGTAATAGTTGCATTATCTTCTTCCCTTCCCGGAAGGACCAACCTCTTCTCCTCCTTACCTCCCCACGACCCCACTCCTTCCTCATCCGCCTCCCCTCGTCCGCCTATCCCCATTCCCAAATACCCCCCACCTCGAAAATCCAAAACCCCACCACCACCATCAAACCCCGCCCTCAAATACCATAACAACTCCAAATACTACAAACCGATCAAACACGACACCGTCATCACCGCCGACGGCGACCGCTCCGTAATCATCGGAGAATCAGGCGTCTCCTACCTCCTCCCCGGCGCTCCATTTGAGTTCCAGTTCAGTTACTCCGAGACTCCCAAAGTTAAGCCCCTGGCGATTCGTGAACCGGCGTTTCTACCGTTCGCGCCTCCCACTATGCCGAGACCGTGGACCGGAAAAGCGCCGTTGAAGAAGTCGAAGAAGAATATTCCTCTTTTTGATTCGTTTAATCCTCCGCCTCCTGGTACGAAAGGAGTTAAGCATGTTGAAATGCCGGGTCCTTTTCCGATTGGGAAGTTTCCAGAAGAAGGAATGAGTAGAAAAGAGATACTTGGGGAGCCTCTTAAGAAGTGGGAGATACAAATGCTTATTAAACCTATGGTGTCTGATAATCGCCAGGTTAATCTCGGAAGGGATGGATTAACGCATAACATGTTGGAGTTGATACAATCGCATTGGAAGCGTTGCCGTGTCTGTAAAATTCGTTGTAAAGGGGTTCCTACTGTTGATATGGATAATGTTTGCCGCCATATTGAGGAGAAAACAGGGGGGAAAATAATTCATCGAGTTGGTGGCGTAGTTTATCTTTTCCGTGGAAGAAATTACAACTACCGTAGTCGTCCACAGTATCCAGTGATGCTCTGGAAGCCAGCTGCTCCTGTATATCCAAAACTTATACAAGATGCTCCAGAAGGATTAACAAAAGCTGAAGCAGATGAATTAAGGATGAAAGGGAAAAGTTTGTTGCCAATATGCAAATTAGTCAAAAATGGAGTATACACAACACTTGTAAAGGATGTCAGAGATGCGTTTGAAGGAAGCCATTTGGTGAAGATTGACTGCAAACTATTGGATCCTAGTGATTACAAGAAAATAGGTGCCAAGTTAAAGGATTTGGTTCCTTGTGTGCTTTTGTCTTTTGATGATCAACAAGTATTGATTTGGAGGGGCAAAAACTGGAAATCAATGTACCCAGAAGTTCCAGTTTTTACTCATGCTAAAGCTGGCATTACAAGGAATTTGGACAGTACAGGCATGGTTAATGACAATCAATCTAAGCATATGGGCAACATGGTCAACTCTAGCCCAAAAATGTTATCTCTATGGAAGAGTGCAATTGAGTCAAACAAAGCTTTGTTGCTAGAAGAATTTAATCTTGGACCTGATGCTCTCTTGGAGAAGGTAGAAGAATTTGAAGGTATTTCACATGCCACAGAGCACTCCCATCCAGCATTTATTTCTTCAAGTGAAGATGCTTTTGAAGGTTCAGAAGACAACTATTCAAATGAAGAAGGCCTAGATAATGTTGATGATTATGACAGAAACAGTGATGACGAAGATGATTTTGATCACTACACCGATGACGAAGACAATGAGTTTAATGATATAGTGGTTACATCTGCTCAACCCGGATCGCTACCAATTGATTTGCTTGTTGGTAAGCTCAAACTAAGGCAAAAGGAGTAAGGAGCTTGTGTAGAAGGCATAAAATGTAAACTTGAAAGAAAAATAGTTAGTTGTATATAATCAAGTTTGGTGATTTTTTTCAAGAAAATATTAAGAGCCTTCGTTTCATTAGTGATGCAATGTTTATAANTTATGATTTGTTTCAATATTTG
TARGET=275,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6423
PRIMER_LEFT_PENALTY=0.046801
PRIMER_RIGHT_PENALTY=0.595491
PRIMER_LEFT_SEQUENCE=AAGAAAGCAGAGTCACACACCA
PRIMER_RIGHT_SEQUENCE=GTTGTTATGGTATTTGAGGGCG
PRIMER_LEFT=36,22
PRIMER_RIGHT=315,22
PRIMER_LEFT_TM=59.953
PRIMER_RIGHT_TM=60.595
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.8609
PRIMER_LEFT_1_PENALTY=0.265389
PRIMER_RIGHT_1_PENALTY=0.595491
PRIMER_LEFT_1_SEQUENCE=AAAGCAGAGTCACACACCACAC
PRIMER_RIGHT_1_SEQUENCE=GTTGTTATGGTATTTGAGGGCG
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=315,22
PRIMER_LEFT_1_TM=60.265
PRIMER_RIGHT_1_TM=60.595
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=277
PRIMER_PAIR_PENALTY_2=0.8889
PRIMER_LEFT_2_PENALTY=0.428401
PRIMER_RIGHT_2_PENALTY=0.460467
PRIMER_LEFT_2_SEQUENCE=TTGCATTATCTTCTTCCCTTCC
PRIMER_RIGHT_2_SEQUENCE=TCGTGTTTGATCGGTTTGTAGT
PRIMER_LEFT_2=134,22
PRIMER_RIGHT_2=344,22
PRIMER_LEFT_2_TM=59.572
PRIMER_RIGHT_2_TM=59.540
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=211
PRIMER_PAIR_PENALTY_3=0.8889
PRIMER_LEFT_3_PENALTY=0.428401
PRIMER_RIGHT_3_PENALTY=0.460467
PRIMER_LEFT_3_SEQUENCE=TTGCATTATCTTCTTCCCTTCC
PRIMER_RIGHT_3_SEQUENCE=GTCGTGTTTGATCGGTTTGTAG
PRIMER_LEFT_3=134,22
PRIMER_RIGHT_3=345,22
PRIMER_LEFT_3_TM=59.572
PRIMER_RIGHT_3_TM=59.540
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.9299
PRIMER_LEFT_4_PENALTY=0.469469
PRIMER_RIGHT_4_PENALTY=0.460467
PRIMER_LEFT_4_SEQUENCE=CCAACCTCTTCTCCTCCTTACC
PRIMER_RIGHT_4_SEQUENCE=TCGTGTTTGATCGGTTTGTAGT
PRIMER_LEFT_4=164,22
PRIMER_RIGHT_4=344,22
PRIMER_LEFT_4_TM=60.469
PRIMER_RIGHT_4_TM=59.540
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=5.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_05783 Ps_contig_mira-and-tgicl-ass_franssen_39918
SEQUENCE=AACTTAAACAAACTTTCTCCATCCCTTAATTTNCACTCCACTAAGCTACGTACCAACCAAACTTCATTTCAAATGGCTGCACCCAAACCACCACATGATCATCATCATCATCCACAACTTCCTCCTGCATCAGAAACTCTCAAGTATCAGACGTGGGTTTTGAAAGTCTTGATCCACTGCGATGGCTGCACGAAAAGAATCAAGAAAATTCTTCAAGGAATTGAAGGGGTTTACACGACAGAAGTTGATTCTATGCAGCACAAGGTTACAGTTACAGGCAACGTTGAAGCTGAGACTCTCATCAAAAAGACTTTCAAGATCAGGAAAATCAGTGGAACTTTGGCCACAAAAACCACCTGAGAAGAAAGATAAAAAACCTTACAACAAAATCAAAGGAAGAGATCAAGGAAGGTGAAAAGAATAGTACTGAACCAGTTGGAGAAAGTAGTCATGAAGATTGTATAGATGATGTAGGTGATGATGGTGGTGAAGAAGATTCAAATCATAATGATGATGATGATGGTGGTGGTGGTGGTGATAATAAAAGTNGAAGGGGGTGACTAAAAAGAAGAAAGAAAAAGAAAAAG
TARGET=98,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1068
PRIMER_LEFT_PENALTY=0.073619
PRIMER_RIGHT_PENALTY=0.033137
PRIMER_LEFT_SEQUENCE=TCCATCCCTTAATTTNCACTCC
PRIMER_RIGHT_SEQUENCE=CTTCTGTCGTGTAAACCCCTTC
PRIMER_LEFT=17,22
PRIMER_RIGHT=243,22
PRIMER_LEFT_TM=59.926
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.75
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.1666
PRIMER_LEFT_1_PENALTY=0.073619
PRIMER_RIGHT_1_PENALTY=0.093026
PRIMER_LEFT_1_SEQUENCE=TCCATCCCTTAATTTNCACTCC
PRIMER_RIGHT_1_SEQUENCE=ACTTTCAAAACCCACGTCTGAT
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=166,22
PRIMER_LEFT_1_TM=59.926
PRIMER_RIGHT_1_TM=59.907
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=150
PRIMER_PAIR_PENALTY_2=0.2833
PRIMER_LEFT_2_PENALTY=0.073619
PRIMER_RIGHT_2_PENALTY=0.209637
PRIMER_LEFT_2_SEQUENCE=TCCATCCCTTAATTTNCACTCC
PRIMER_RIGHT_2_SEQUENCE=TGTAAACCCCTTCAATTCCTTG
PRIMER_LEFT_2=17,22
PRIMER_RIGHT_2=234,22
PRIMER_LEFT_2_TM=59.926
PRIMER_RIGHT_2_TM=60.210
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.75
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=0.2990
PRIMER_LEFT_3_PENALTY=0.073619
PRIMER_RIGHT_3_PENALTY=0.225403
PRIMER_LEFT_3_SEQUENCE=TCCATCCCTTAATTTNCACTCC
PRIMER_RIGHT_3_SEQUENCE=CCTTGTGCTGCATAGAATCAAC
PRIMER_LEFT_3=17,22
PRIMER_RIGHT_3=264,22
PRIMER_LEFT_3_TM=59.926
PRIMER_RIGHT_3_TM=59.775
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=248
PRIMER_PAIR_PENALTY_4=0.2990
PRIMER_LEFT_4_PENALTY=0.073619
PRIMER_RIGHT_4_PENALTY=0.225403
PRIMER_LEFT_4_SEQUENCE=TCCATCCCTTAATTTNCACTCC
PRIMER_RIGHT_4_SEQUENCE=AACCTTGTGCTGCATAGAATCA
PRIMER_LEFT_4=17,22
PRIMER_RIGHT_4=266,22
PRIMER_LEFT_4_TM=59.926
PRIMER_RIGHT_4_TM=59.775
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=250
=
PRIMER_SEQUENCE_ID=SSR_05784 Ps_contig_mira-and-tgicl-ass_franssen_39918
SEQUENCE=AACTTAAACAAACTTTCTCCATCCCTTAATTTNCACTCCACTAAGCTACGTACCAACCAAACTTCATTTCAAATGGCTGCACCCAAACCACCACATGATCATCATCATCATCCACAACTTCCTCCTGCATCAGAAACTCTCAAGTATCAGACGTGGGTTTTGAAAGTCTTGATCCACTGCGATGGCTGCACGAAAAGAATCAAGAAAATTCTTCAAGGAATTGAAGGGGTTTACACGACAGAAGTTGATTCTATGCAGCACAAGGTTACAGTTACAGGCAACGTTGAAGCTGAGACTCTCATCAAAAAGACTTTCAAGATCAGGAAAATCAGTGGAACTTTGGCCACAAAAACCACCTGAGAAGAAAGATAAAAAACCTTACAACAAAATCAAAGGAAGAGATCAAGGAAGGTGAAAAGAATAGTACTGAACCAGTTGGAGAAAGTAGTCATGAAGATTGTATAGATGATGTAGGTGATGATGGTGGTGAAGAAGATTCAAATCATAATGATGATGATGATGGTGGTGGTGGTGGTGATAATAAAAGTNGAAGGGGGTGACTAAAAAGAAGAAAGAAAAAGAAAAAG
TARGET=508,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2505
PRIMER_LEFT_PENALTY=0.225403
PRIMER_RIGHT_PENALTY=0.025128
PRIMER_LEFT_SEQUENCE=TGATTCTATGCAGCACAAGGTT
PRIMER_RIGHT_SEQUENCE=TTTATTATCACCACCACCACCA
PRIMER_LEFT=245,22
PRIMER_RIGHT=544,22
PRIMER_LEFT_TM=59.775
PRIMER_RIGHT_TM=59.975
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.3345
PRIMER_LEFT_1_PENALTY=0.309418
PRIMER_RIGHT_1_PENALTY=0.025128
PRIMER_LEFT_1_SEQUENCE=GGAAGAGATCAAGGAAGGTGAA
PRIMER_RIGHT_1_SEQUENCE=TTTATTATCACCACCACCACCA
PRIMER_LEFT_1=394,22
PRIMER_RIGHT_1=544,22
PRIMER_LEFT_1_TM=59.691
PRIMER_RIGHT_1_TM=59.975
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=151
PRIMER_PAIR_PENALTY_2=0.5257
PRIMER_LEFT_2_PENALTY=0.500551
PRIMER_RIGHT_2_PENALTY=0.025128
PRIMER_LEFT_2_SEQUENCE=AGGAAAATCAGTGGAACTTTGG
PRIMER_RIGHT_2_SEQUENCE=TTTATTATCACCACCACCACCA
PRIMER_LEFT_2=321,22
PRIMER_RIGHT_2=544,22
PRIMER_LEFT_2_TM=59.499
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.6224
PRIMER_LEFT_3_PENALTY=0.500551
PRIMER_RIGHT_3_PENALTY=0.121866
PRIMER_LEFT_3_SEQUENCE=AGGAAAATCAGTGGAACTTTGG
PRIMER_RIGHT_3_SEQUENCE=TTCTTTTTAGTCACCCCCTTCN
PRIMER_LEFT_3=321,22
PRIMER_RIGHT_3=569,22
PRIMER_LEFT_3_TM=59.499
PRIMER_RIGHT_3_TM=60.122
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=7.6500
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.6740
PRIMER_LEFT_4_PENALTY=0.648872
PRIMER_RIGHT_4_PENALTY=0.025128
PRIMER_LEFT_4_SEQUENCE=ACAGTTACAGGCAACGTTGAAG
PRIMER_RIGHT_4_SEQUENCE=TTTATTATCACCACCACCACCA
PRIMER_LEFT_4=267,22
PRIMER_RIGHT_4=544,22
PRIMER_LEFT_4_TM=59.351
PRIMER_RIGHT_4_TM=59.975
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_05785 Ps_contig_mira-and-tgicl-ass_franssen_39918
SEQUENCE=AACTTAAACAAACTTTCTCCATCCCTTAATTTNCACTCCACTAAGCTACGTACCAACCAAACTTCATTTCAAATGGCTGCACCCAAACCACCACATGATCATCATCATCATCCACAACTTCCTCCTGCATCAGAAACTCTCAAGTATCAGACGTGGGTTTTGAAAGTCTTGATCCACTGCGATGGCTGCACGAAAAGAATCAAGAAAATTCTTCAAGGAATTGAAGGGGTTTACACGACAGAAGTTGATTCTATGCAGCACAAGGTTACAGTTACAGGCAACGTTGAAGCTGAGACTCTCATCAAAAAGACTTTCAAGATCAGGAAAATCAGTGGAACTTTGGCCACAAAAACCACCTGAGAAGAAAGATAAAAAACCTTACAACAAAATCAAAGGAAGAGATCAAGGAAGGTGAAAAGAATAGTACTGAACCAGTTGGAGAAAGTAGTCATGAAGATTGTATAGATGATGTAGGTGATGATGGTGGTGAAGAAGATTCAAATCATAATGATGATGATGATGGTGGTGGTGGTGGTGATAATAAAAGTNGAAGGGGGTGACTAAAAAGAAGAAAGAAAAAGAAAAAG
TARGET=523,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6224
PRIMER_LEFT_PENALTY=0.500551
PRIMER_RIGHT_PENALTY=0.121866
PRIMER_LEFT_SEQUENCE=AGGAAAATCAGTGGAACTTTGG
PRIMER_RIGHT_SEQUENCE=TTCTTTTTAGTCACCCCCTTCN
PRIMER_LEFT=321,22
PRIMER_RIGHT=569,22
PRIMER_LEFT_TM=59.499
PRIMER_RIGHT_TM=60.122
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=7.6500
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.75
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.6812
PRIMER_LEFT_1_PENALTY=0.444466
PRIMER_RIGHT_1_PENALTY=0.236697
PRIMER_LEFT_1_SEQUENCE=ATGATGTAGGTGATGATGGTGG
PRIMER_RIGHT_1_SEQUENCE=TCTTTTTAGTCACCCCCTTCNA
PRIMER_LEFT_1=465,22
PRIMER_RIGHT_1=568,22
PRIMER_LEFT_1_TM=59.556
PRIMER_RIGHT_1_TM=60.237
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.50
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.6250
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=104
PRIMER_PAIR_PENALTY_2=0.7372
PRIMER_LEFT_2_PENALTY=0.500551
PRIMER_RIGHT_2_PENALTY=0.236697
PRIMER_LEFT_2_SEQUENCE=AGGAAAATCAGTGGAACTTTGG
PRIMER_RIGHT_2_SEQUENCE=TCTTTTTAGTCACCCCCTTCNA
PRIMER_LEFT_2=321,22
PRIMER_RIGHT_2=568,22
PRIMER_LEFT_2_TM=59.499
PRIMER_RIGHT_2_TM=60.237
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.50
PRIMER_LEFT_2_END_STABILITY=8.8000
PRIMER_RIGHT_2_END_STABILITY=7.6250
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=0.8111
PRIMER_LEFT_3_PENALTY=0.118777
PRIMER_RIGHT_3_PENALTY=0.692285
PRIMER_LEFT_3_SEQUENCE=TGATGTAGGTGATGATGGTGGT
PRIMER_RIGHT_3_SEQUENCE=TTTTAGTCACCCCCTTCNACTT
PRIMER_LEFT_3=466,22
PRIMER_RIGHT_3=565,22
PRIMER_LEFT_3_TM=60.119
PRIMER_RIGHT_3_TM=59.308
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.50
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.3750
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_3=100
PRIMER_PAIR_PENALTY_4=0.8639
PRIMER_LEFT_4_PENALTY=0.742018
PRIMER_RIGHT_4_PENALTY=0.121866
PRIMER_LEFT_4_SEQUENCE=CACAAAAACCACCTGAGAAGAA
PRIMER_RIGHT_4_SEQUENCE=TTCTTTTTAGTCACCCCCTTCN
PRIMER_LEFT_4=344,22
PRIMER_RIGHT_4=569,22
PRIMER_LEFT_4_TM=59.258
PRIMER_RIGHT_4_TM=60.122
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.6500
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_4=226
=
PRIMER_SEQUENCE_ID=SSR_05786 Ps_contig_mira-and-tgicl-ass_franssen_39922
SEQUENCE=ATTAAAGTTCATAATGTAAAGTCAAATTAAGGATAAATATCTATTATACATGTGGCAATATATATTTTAGCATATATAAAGTAATAATACCGTTTGRTCGGGGATGCACACGACACCTTTTGCTTCTCTTCCATTTAACACATAACTGGCATATGAATCCAAACACGTCTCTCTCTCTCTCTTTCTCTCTCTCCCAACTCTTAAGTTCAAAGCTTCAGCCTCCAATGCTTTTTAAACAATTACAAACTTTGCAGAATTAGATCAAAAGAAACTAGAACAAAAGAAAAAAACAGCTAGCTAAATTTATAGAGATTGCTGAAAAGATTCTAAAGTTGCACGTTATTCTTTGTCATGTGAAGTATCAAAACTGTT
TARGET=168,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05787 Ps_contig_mira-and-tgicl-ass_franssen_39922
SEQUENCE=ATTAAAGTTCATAATGTAAAGTCAAATTAAGGATAAATATCTATTATACATGTGGCAATATATATTTTAGCATATATAAAGTAATAATACCGTTTGRTCGGGGATGCACACGACACCTTTTGCTTCTCTTCCATTTAACACATAACTGGCATATGAATCCAAACACGTCTCTCTCTCTCTCTTTCTCTCTCTCCCAACTCTTAAGTTCAAAGCTTCAGCCTCCAATGCTTTTTAAACAATTACAAACTTTGCAGAATTAGATCAAAAGAAACTAGAACAAAAGAAAAAAACAGCTAGCTAAATTTATAGAGATTGCTGAAAAGATTCTAAAGTTGCACGTTATTCTTTGTCATGTGAAGTATCAAAACTGTT
TARGET=184,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05788 Ps_contig_mira-and-tgicl-ass_franssen_39934
SEQUENCE=CGGGGGTGTGCCGAAGCTAAAGAGATCGGAGCTTGAAGCAGCGTGCGAAGATTTTAGTAATGTAATCGGTACTTCACCCGTTGGAAGCATTTACACAGTGTACAAAGGGACTCTATCTAGCGGTGTCGAAATAGCCGTAGCTTCTGTTACAGTGACATCGTTGAAAGATTGGTCAAAGACTTGCGAAGTCCAATTTCGTAAGAAGATAGATACATTATCAAAGATGAACCACAAGAATTTCGTAAATCTTCTTGGATTTTGCGAAGAAGACGAGCCATTCACCAGAATGGTGGTTTTTGAATACGCCCCAAATGGAACACTCTTCGAGCATTTACACATAAAAGAAGCCGAACATTTGGATTGGGGAACAAGACTTAGAGTTGCAATTGGCACGGCTTACTGCCTGCAACATATGCACCAGCTAAACCCTCCGCTCGCCCATAGCAGCCTAAACACTTCATCCGTTCAACTCACTGACGACTACGCCGCCAAAATCTCGGACCTAAGTTTCTTGAAAGAAATAGCTTCACCCGATATAAAATCTTCTGCCAAGAAACGCACGGACGCGACATTAGCAAGTAACATTTACAGCTTCGGTATCATGTTACTCGAAACTGTAACGGGCCGTCTTCCTTATTCCAATGATCAAGACGGTTCACTTGAAAACTGGGCTTCAAACTATTTACAAGGGAACCAATCTCTCAAAGAAATAGTGGATCCAACACTAGTATCCTTCCAAGAAGAACAATTAGTACAAATCGGCGCATTGATTAAGTCCTGTGTCAATGCTGATCAAGAGCAAAGACCAAACAAATAAAACAAGTTTGCGAAAGATTAAGAGAGATAACGAAAATAAGTCCCGAAGTAGCTGTTCCAAAACTTTCACCACTTTGGTGGGCAGAACTCGAGATTGCTTCTTTCGACGCCAGCTGAAATCGCGAAAAGAAATTGTGAGTATGGTGTTTGAATCAATCTCGTCGGAGATGGCGCTGAAAAGATACTCACCTGTAAACTGATACCTCTTGTATCTATTGTAGGGGAGTAATAAATCACATTGTACATAAGGGAGATAAAAAGAACCAATTTTTTTGTGTGCTTCGTTTGGTTATGGTTTCATGGAATTGCACCAGCAAAAGGTGCCATATATTAGTTAATTTCATTTGTTGAGTAAGTTATTTTGTTGGTTAAAGATATTTTTGATTATATTTCTTACTATAATAATAATACTTTCACTTANNTTAAGGT
TARGET=1215,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05789 Ps_contig_mira-and-tgicl-ass_franssen_39945
SEQUENCE=TAGCCAAGTAAACTTAATTAACCCTAACGAAAATACCGGTTAATGTCAATGAATTAGTATGAACAAGTAAAGCAAAAAAAACAAAGTTAACTCTTAAACCTTAGTTGACTAGATGAACATATACTCCCTTCCTGTATTTTGTCCTATGCTTGGGTATGGGCCTTTGGCCTCCACGCCTGCTGCTGCTGACTTTGACGACCTTTCGTAGGTAGACTTGAAGTCGAACCATTGGAGCTTGCTCCTTGCTTTGAAGTAGAGCCGTTGGAGCTTGCTCCTTGCTTTGAAGTCGAACCGTTGGAGTTGGCTCTGTGCTTTGAAGTCGAACCGTTGGAATTGGCTCCTCGCTTTGAAGTCGAACCGTTGGAATTGGCTCCTTGCTTTGAAGTCGAACCGTTGGAATTGGCTCCTTGCTTTGAAGTCGAACCGTTGGAGCTGGCTCCCTGCCTGCCATTTTGAACATGGCGTTTTGAATGTGGGTGTGGGAGCAATCCCCTCTGAACCTGTGGTTGAGTTAGATGAGGGTAACCGCCGTTGTAATTTGGAATAGAAGGCCTGACATTATAATAAGGAATGACAGGTTTGGCAATAAGTCTCAACACATGTGGTGGTACTAATTGGGTCAGGATAGAGCTTTGGTTCTGATTGTTTGATATAAGTAAGTTGTACGTTGTTAGAAATGCTTCGCCTATTCTTTTTCAGCTGT
TARGET=177,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4667
PRIMER_LEFT_PENALTY=0.990369
PRIMER_RIGHT_PENALTY=0.476376
PRIMER_LEFT_SEQUENCE=TTTTGTCCTATGCTTGGGTATG
PRIMER_RIGHT_SEQUENCE=ACTTCAAAGCACAGAGCCAACT
PRIMER_LEFT=136,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=59.010
PRIMER_RIGHT_TM=60.476
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=1.5498
PRIMER_LEFT_1_PENALTY=1.073458
PRIMER_RIGHT_1_PENALTY=0.476376
PRIMER_LEFT_1_SEQUENCE=TTTGTCCTATGCTTGGGTATGG
PRIMER_RIGHT_1_SEQUENCE=ACTTCAAAGCACAGAGCCAACT
PRIMER_LEFT_1=137,22
PRIMER_RIGHT_1=319,22
PRIMER_LEFT_1_TM=61.073
PRIMER_RIGHT_1_TM=60.476
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=183
PRIMER_PAIR_PENALTY_2=1.6454
PRIMER_LEFT_2_PENALTY=1.169021
PRIMER_RIGHT_2_PENALTY=0.476376
PRIMER_LEFT_2_SEQUENCE=TTGTCCTATGCTTGGGTATGG
PRIMER_RIGHT_2_SEQUENCE=ACTTCAAAGCACAGAGCCAACT
PRIMER_LEFT_2=138,21
PRIMER_RIGHT_2=319,22
PRIMER_LEFT_2_TM=59.831
PRIMER_RIGHT_2_TM=60.476
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=182
PRIMER_PAIR_PENALTY_3=1.6791
PRIMER_LEFT_3_PENALTY=1.353331
PRIMER_RIGHT_3_PENALTY=0.325733
PRIMER_LEFT_3_SEQUENCE=CTCCCTTCCTGTATTTTGTCCT
PRIMER_RIGHT_3_SEQUENCE=GGCTCTACTTCAAAGCAAGGAG
PRIMER_LEFT_3=123,22
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=58.647
PRIMER_RIGHT_3_TM=59.674
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=137
PRIMER_PAIR_PENALTY_4=1.8131
PRIMER_LEFT_4_PENALTY=1.487417
PRIMER_RIGHT_4_PENALTY=0.325733
PRIMER_LEFT_4_SEQUENCE=ACTCCCTTCCTGTATTTTGTCC
PRIMER_RIGHT_4_SEQUENCE=GGCTCTACTTCAAAGCAAGGAG
PRIMER_LEFT_4=122,22
PRIMER_RIGHT_4=259,22
PRIMER_LEFT_4_TM=58.513
PRIMER_RIGHT_4_TM=59.674
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_05790 Ps_contig_mira-and-tgicl-ass_franssen_39946
SEQUENCE=GTCGGGGTTGTTGTATTTTACGGGGTAGAAGAAGATGGTGAGAGTTGGTTAGAGTTTTTGAGAATCGAAGAAGACGATGGTGAGAGTTTGTTAGAAATTTTGTGAACCGAAGAAGACGATRGAGAGAGTTGGTTAGAAATTTTGTAGAGAGAGAGAAAATTCGTTGTTAATCAAGCTTTGCTTTGTGTTTTGATTCATTTGCCGTTTGCACTTCATTTTTGGCTTGTTTGCCGCGTTGTCGTTCATATCCGCTTGCAATGCGTTTTCCATTTTACGTTTCACTTGGCGCGAACTTGCGTTTGGCGTTTTATATCGGGACTTTCTTCGCTTTAATTTTCTATTCTAAGTTGCAACTGCATTTTGGCTTAATNNAGCTTGTTGATGTGCGTTGCAGCGTGTGGCTTCATCTACAGCTTCCAATTTGCAACTCACTCATTATTTTCTTGTTGCGCTTGGCTTTGGTTTGCTGTTTTGATGTTG
TARGET=146,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05791 Ps_contig_mira-and-tgicl-ass_franssen_39947
SEQUENCE=TTTCATCTGGAGATTATGTCATCACAAGAAAGATCGAAAGAACTTCATTGAATCAAATAGTTTGAACAAAAGAGAGAGCCATCATGAAGGAAGCAATCAATTTGATCAAGGAAGAAAGAAAAAGGAAGAAAAGATTTGTTTGATTGGGTCGATCATCCATATCTAGCTTCTGATGCTGTTGAAAATGGATGGTCAAGATTTGCTTTCACAAGCTATAAAAGTGATTCTATGTCGTCAAAAAAATCAACTTCTTCGACCCTTTTAGGAGCTTGTGGAGGTGGTGGTGGTGAGTATGGAAGAGAAGAAAATGAGGTTGAAATAAGTTGGGAAGTCTGTCAAGGTTCAAATGAATTCATGCAGAAGATAAGACTTAATTCTGGTTTGAAAAAGTGTTTTTTTCATCCAAACAATGCTTTAATGAATGTTCATTCTGTTGTTAGGACTTGTTTGCCTCTTCCTGGTCCTAGTTTAGGGAACCATGTTTTTCCACAAGAAGCTTATTTTGAGATTACTATATTGTATTCTTGTGGTGGAGATGATTGTGAAGTTGTTGGAAGAAGAGAAGGAGAAAAGACAAAATTGCTTATTGAAAAAAGTTTAGAATGATGAAGGTGATTTGAAAAGTGTTGAAGAAATGAAAGTTGAAGGAAAAGATGGATCTGTGATGGTGTCATTAGGGTTAAGCTATGGTGGAAGTGTTCCTTTAAGAGTTCCAGGAAGTTACCCTAGAAGCATAGGGTTCAACTCCAATGGTTCTGTTTTTCTTGATGGAATGAAACTCGTATTTGAATCCGACAAAGTGCAATGGATAGGAAGGGACACAGTAATTGGTTGTGGATTTGATCCAAGAAAAAAGAAAGTTTTCTTCACATTAGACTCAGAACTGGTGCATGTAATTCACTGTCAATCAGAGGAATTCAGCACACCACTCTGTCCAACAATTGCGGCAAATATAGACATTATGATTTTAGTTAATTTTGGGCAAGTTGCATTCAAATATGCGCCTGCAAATGCGCAAAGAACGCCGAATCCGTGTTTTATAGCACCACTTGTACATTCCCCTCGGTGCTACGCTTGGATTTGATGACAGTAAGGAACTATTCTCGATGGGAAGGATTGATTCGCCGTGGCAGAATCGATCCGCGACGAAAGGAAATAACAACGGTGGGAATAATAATAATCTAGCATTTGATTTTGATGAGGAATCTGAAGCTGATTTGTTTGAAATTGTGTTGGATGGTTCAGAAAAATCACCATACTCAGTGTCCTTATCATAAGTTACTGCAAACAATGATGTATAAACCACATGTAGTTATTTTGTTTCTGCAATGAAATGTGTGTTTGTTTAGGCCTCTCACACATTGTCAATGTTTCGTTTCGTTTTTTTCAATTTTACCCTTAGTTCGAAGAATAAATACTTATAACTAAAAATTAAATACAAATTATTTTTAATAGTAAAAAAAATATTCATTAAATACGTGTGTCAACAAAGGATTCAAT
TARGET=277,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0233
PRIMER_LEFT_PENALTY=0.006945
PRIMER_RIGHT_PENALTY=0.016312
PRIMER_LEFT_SEQUENCE=TGAACAAAAGAGAGAGCCATCA
PRIMER_RIGHT_SEQUENCE=GAACCTTGACAGACTTCCCAAC
PRIMER_LEFT=62,22
PRIMER_RIGHT=343,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=60.016
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.1273
PRIMER_LEFT_1_PENALTY=0.110942
PRIMER_RIGHT_1_PENALTY=0.016312
PRIMER_LEFT_1_SEQUENCE=GCTTCTGATGCTGTTGAAAATG
PRIMER_RIGHT_1_SEQUENCE=GAACCTTGACAGACTTCCCAAC
PRIMER_LEFT_1=165,22
PRIMER_RIGHT_1=343,22
PRIMER_LEFT_1_TM=59.889
PRIMER_RIGHT_1_TM=60.016
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=179
PRIMER_PAIR_PENALTY_2=0.2698
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.016312
PRIMER_LEFT_2_SEQUENCE=CTTCTGATGCTGTTGAAAATGG
PRIMER_RIGHT_2_SEQUENCE=GAACCTTGACAGACTTCCCAAC
PRIMER_LEFT_2=166,22
PRIMER_RIGHT_2=343,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.016
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=0.3486
PRIMER_LEFT_3_PENALTY=0.110942
PRIMER_RIGHT_3_PENALTY=0.237699
PRIMER_LEFT_3_SEQUENCE=GCTTCTGATGCTGTTGAAAATG
PRIMER_RIGHT_3_SEQUENCE=GGAAGAGGCAAACAAGTCCTAA
PRIMER_LEFT_3=165,22
PRIMER_RIGHT_3=457,22
PRIMER_LEFT_3_TM=59.889
PRIMER_RIGHT_3_TM=59.762
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.3649
PRIMER_LEFT_4_PENALTY=0.348633
PRIMER_RIGHT_4_PENALTY=0.016312
PRIMER_LEFT_4_SEQUENCE=GAGAGAGCCATCATGAAGGAAG
PRIMER_RIGHT_4_SEQUENCE=GAACCTTGACAGACTTCCCAAC
PRIMER_LEFT_4=71,22
PRIMER_RIGHT_4=343,22
PRIMER_LEFT_4_TM=60.349
PRIMER_RIGHT_4_TM=60.016
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_05792 Ps_contig_mira-and-tgicl-ass_franssen_39947
SEQUENCE=TTTCATCTGGAGATTATGTCATCACAAGAAAGATCGAAAGAACTTCATTGAATCAAATAGTTTGAACAAAAGAGAGAGCCATCATGAAGGAAGCAATCAATTTGATCAAGGAAGAAAGAAAAAGGAAGAAAAGATTTGTTTGATTGGGTCGATCATCCATATCTAGCTTCTGATGCTGTTGAAAATGGATGGTCAAGATTTGCTTTCACAAGCTATAAAAGTGATTCTATGTCGTCAAAAAAATCAACTTCTTCGACCCTTTTAGGAGCTTGTGGAGGTGGTGGTGGTGAGTATGGAAGAGAAGAAAATGAGGTTGAAATAAGTTGGGAAGTCTGTCAAGGTTCAAATGAATTCATGCAGAAGATAAGACTTAATTCTGGTTTGAAAAAGTGTTTTTTTCATCCAAACAATGCTTTAATGAATGTTCATTCTGTTGTTAGGACTTGTTTGCCTCTTCCTGGTCCTAGTTTAGGGAACCATGTTTTTCCACAAGAAGCTTATTTTGAGATTACTATATTGTATTCTTGTGGTGGAGATGATTGTGAAGTTGTTGGAAGAAGAGAAGGAGAAAAGACAAAATTGCTTATTGAAAAAAGTTTAGAATGATGAAGGTGATTTGAAAAGTGTTGAAGAAATGAAAGTTGAAGGAAAAGATGGATCTGTGATGGTGTCATTAGGGTTAAGCTATGGTGGAAGTGTTCCTTTAAGAGTTCCAGGAAGTTACCCTAGAAGCATAGGGTTCAACTCCAATGGTTCTGTTTTTCTTGATGGAATGAAACTCGTATTTGAATCCGACAAAGTGCAATGGATAGGAAGGGACACAGTAATTGGTTGTGGATTTGATCCAAGAAAAAAGAAAGTTTTCTTCACATTAGACTCAGAACTGGTGCATGTAATTCACTGTCAATCAGAGGAATTCAGCACACCACTCTGTCCAACAATTGCGGCAAATATAGACATTATGATTTTAGTTAATTTTGGGCAAGTTGCATTCAAATATGCGCCTGCAAATGCGCAAAGAACGCCGAATCCGTGTTTTATAGCACCACTTGTACATTCCCCTCGGTGCTACGCTTGGATTTGATGACAGTAAGGAACTATTCTCGATGGGAAGGATTGATTCGCCGTGGCAGAATCGATCCGCGACGAAAGGAAATAACAACGGTGGGAATAATAATAATCTAGCATTTGATTTTGATGAGGAATCTGAAGCTGATTTGTTTGAAATTGTGTTGGATGGTTCAGAAAAATCACCATACTCAGTGTCCTTATCATAAGTTACTGCAAACAATGATGTATAAACCACATGTAGTTATTTTGTTTCTGCAATGAAATGTGTGTTTGTTTAGGCCTCTCACACATTGTCAATGTTTCGTTTCGTTTTTTTCAATTTTACCCTTAGTTCGAAGAATAAATACTTATAACTAAAAATTAAATACAAATTATTTTTAATAGTAAAAAAAATATTCATTAAATACGTGTGTCAACAAAGGATTCAAT
TARGET=1170,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1206
PRIMER_LEFT_PENALTY=0.075795
PRIMER_RIGHT_PENALTY=0.044832
PRIMER_LEFT_SEQUENCE=CTATTCTCGATGGGAAGGATTG
PRIMER_RIGHT_SEQUENCE=TCAGCTTCAGATTCCTCATCAA
PRIMER_LEFT=1097,22
PRIMER_RIGHT=1215,22
PRIMER_LEFT_TM=59.924
PRIMER_RIGHT_TM=59.955
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.4701
PRIMER_LEFT_1_PENALTY=0.425301
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_LEFT_1_SEQUENCE=CCGTGTTTTATAGCACCACTTG
PRIMER_RIGHT_1_SEQUENCE=TCAGCTTCAGATTCCTCATCAA
PRIMER_LEFT_1=1030,22
PRIMER_RIGHT_1=1215,22
PRIMER_LEFT_1_TM=59.575
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=0.4906
PRIMER_LEFT_2_PENALTY=0.445740
PRIMER_RIGHT_2_PENALTY=0.044832
PRIMER_LEFT_2_SEQUENCE=GAACTATTCTCGATGGGAAGGA
PRIMER_RIGHT_2_SEQUENCE=TCAGCTTCAGATTCCTCATCAA
PRIMER_LEFT_2=1094,22
PRIMER_RIGHT_2=1215,22
PRIMER_LEFT_2_TM=59.554
PRIMER_RIGHT_2_TM=59.955
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=122
PRIMER_PAIR_PENALTY_3=0.6233
PRIMER_LEFT_3_PENALTY=0.075795
PRIMER_RIGHT_3_PENALTY=0.547508
PRIMER_LEFT_3_SEQUENCE=CTATTCTCGATGGGAAGGATTG
PRIMER_RIGHT_3_SEQUENCE=CAGCTTCAGATTCCTCATCAAA
PRIMER_LEFT_3=1097,22
PRIMER_RIGHT_3=1214,22
PRIMER_LEFT_3_TM=59.924
PRIMER_RIGHT_3_TM=59.452
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=0.6233
PRIMER_LEFT_4_PENALTY=0.075795
PRIMER_RIGHT_4_PENALTY=0.547508
PRIMER_LEFT_4_SEQUENCE=CTATTCTCGATGGGAAGGATTG
PRIMER_RIGHT_4_SEQUENCE=CAAATCAGCTTCAGATTCCTCA
PRIMER_LEFT_4=1097,22
PRIMER_RIGHT_4=1219,22
PRIMER_LEFT_4_TM=59.924
PRIMER_RIGHT_4_TM=59.452
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=123
=
PRIMER_SEQUENCE_ID=SSR_05793 Ps_contig_mira-and-tgicl-ass_franssen_39948
SEQUENCE=AAAAAAAAAAAGCATTTTCATTCAAGATAAATAAAAAANCATAAAAACCATTTACAATGTTAAACAATATATGTTCCAAACATGTTTCTGGGAGAATTTGTTTCCTTTAAGGCCTATTTGCTATCCTAATTCCTAACTCTCAATTTTCACAAGTTAAACCAATTAGACTAGCTCAACAATCAACAAGAATAGACCTTCTTTCCTTACAAAACATCCCACACAAAATGTTTGTTTCAGAATAACCTGTCCCTGAAAACATCACTTAATTAAACTACTCAATTACTTGTCTTCATCAGCACCACCGCGAATCCGATTCTGAAGATGTTCCAAGCTAGCCACTCGCGGCAAGGAGTTTGTTCTCCCTATCTTGTTCCCTGCTGCAGTGTTCATCTGCANAGCACAGATGCATCAGCTGATGACTCAGAAGGGCTTCCATCAAATAATGACATTCCCATTCCCATTGATGATAACTCAGATATTTCATTAAACATCGGATTCAATCCAGTAAATCTCTTAACCGTCTCTTCAGCCATCTTCACCTTTGCTCTTAAGGTTTCAACATCAGCTTTTAATATTCTGTTGTCAACAGCAGAATCATTGTACTTTTGAGTCACATCTGTTAAATTCTTTAACAATGAAGATTTTTCACCTCTTAATTGAGAAACCTGTGTTTCAAGCTCAGATAAATGAGCTTGTTTTCTTCGTCTCGAACGTCTAGCAGACTCCCTATTAGAGAGCATCCTTCTTACTCTTTTTGCATCAGAAGGATTCATGTCTCCATTCATATTAATCTCTCCCTCAGCTTCTTCATCATCTGATGAACCACTTGTTGTTGACCTGATTGAAACAACAGGCTTCTTTTGACCTACTTTCATCCCATCTTTATTTAGCAATTTAGATGAATCATTTCCAGAAGCTCCAAGTTCAGAAGCATAAGCTGCATGTGATCCATTGTCTGGTTCCTGAGATTTCACCAAAGTTCCCCGAGACATGGCGACTGCGGCGCAGGCGAGATCGAGCTTGGTTTTGAGAATAGCATAGTAATCCTGAGAATCATTGATATTATTATTATTATTATTATTGTTATTGATTTTGAGTTTGAGATCAAGGTTGGAAGAAGAAGTTGAAGTTGAAGTTGAAGAGGAAGAACAAGGCTTTGCATTAGAAGTATTTGCAGCTTCTTGTTCTTGTTGTTGAAGAAACTGTTGAAAAGCCCATTCAGAATCACTTCTATTCATCTTTGATTCTCCTTCTTTACCAGCCTTAGATGTTAACCAATATTGTTCAGTAATTTCTGCAATTGAAAACACTCTTTCCATTCTCTTTTTCTCTAAGCTTTTGTTGTTATCATATCACAATGACACTATTATAACAGTGTTGTTAATTCTAATTCTTGCATAATAATTCACAATCAAGTTTGAATCTTCAACAAGATTTTATTACCATAAATAAATTGATATGTGACAT
TARGET=1061,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0341
PRIMER_LEFT_PENALTY=0.020747
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=GTCTGGTTCCTGAGATTTCACC
PRIMER_RIGHT_SEQUENCE=GAATGGGCTTTTCAACAGTTTC
PRIMER_LEFT=953,22
PRIMER_RIGHT=1219,22
PRIMER_LEFT_TM=59.979
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.0370
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.013401
PRIMER_LEFT_1_SEQUENCE=CTGAGATTTCACCAAAGTTCCC
PRIMER_RIGHT_1_SEQUENCE=GAATGGGCTTTTCAACAGTTTC
PRIMER_LEFT_1=962,22
PRIMER_RIGHT_1=1219,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.987
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.0370
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.013401
PRIMER_LEFT_2_SEQUENCE=CCTGAGATTTCACCAAAGTTCC
PRIMER_RIGHT_2_SEQUENCE=GAATGGGCTTTTCAACAGTTTC
PRIMER_LEFT_2=961,22
PRIMER_RIGHT_2=1219,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=59.987
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=259
PRIMER_PAIR_PENALTY_3=0.0370
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.013401
PRIMER_LEFT_3_SEQUENCE=GGTTCCTGAGATTTCACCAAAG
PRIMER_RIGHT_3_SEQUENCE=GAATGGGCTTTTCAACAGTTTC
PRIMER_LEFT_3=957,22
PRIMER_RIGHT_3=1219,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=59.987
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=0.0693
PRIMER_LEFT_4_PENALTY=0.020747
PRIMER_RIGHT_4_PENALTY=0.048570
PRIMER_LEFT_4_SEQUENCE=GTCTGGTTCCTGAGATTTCACC
PRIMER_RIGHT_4_SEQUENCE=GATTCTGAATGGGCTTTTCAAC
PRIMER_LEFT_4=953,22
PRIMER_RIGHT_4=1225,22
PRIMER_LEFT_4_TM=59.979
PRIMER_RIGHT_4_TM=59.951
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_05794 Ps_contig_mira-and-tgicl-ass_franssen_39951
SEQUENCE=ATGGAAGTTGATGTATAGTACGGGGAGATTCAAACATCCCTCACCACATCTCGCTTTCTCTCTCTAGCTTTCTCTATCTCTCTCTTGCAACTTTCTTTTCTTTTCCAAATATTTACATAAGAAAAAGAAAGCTCTACTTAAAATTGTCCATCCTATTCAATTGAAGGACTAGTTTAATTTTCTCTTGAGTATTTATTACAAGATGTTGTGAAGCCTAGAGAAGCAAATATGCATGAGATGGCTTTCTTCCAAGCAAATTTTATGTTACAAACTCCTCATCACCATGAAGATCATCATCATCATCAACCACCTTCACTCAACTCAATTCTACCTCAAGATTATCATGGGGGGCCATCATTTCTAGGGAAGAGATCTATGTCATTCTCATCCGGAATCGAACTCGGAGAAGAAGCCAATATAGCCGAGGAAGATTTATCAGATGATGGATCACAAGCGGGTGAGAAGAAGAGGAGGCTCAACATGGAACAAGTGAAAACACTTGAGAAAAGTTTTGAGCTTGGGAACAAGCTAGAGCCTGAGAGGAAAATGCAGCTTGCAAGAGCTCTTAACTTGCAACCTAGACAAGTTGCTATATGGTTTACAAAATAGAAGAGCTAGATGGAAGACAAAGCAAATTAGGAAAAAAGACTATGATGTACNTTAAAAGACAATATGATGCTGTTAAGTTAGACAATGATGCACTTCAAGCTACAAAAACCAAAACTTCAAGCA
TARGET=290,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3436
PRIMER_LEFT_PENALTY=0.189596
PRIMER_RIGHT_PENALTY=0.154023
PRIMER_LEFT_SEQUENCE=TGTTGTGAAGCCTAGAGAAGCA
PRIMER_RIGHT_SEQUENCE=TTTCACTTGTTCCATGTTGAGC
PRIMER_LEFT=203,22
PRIMER_RIGHT=494,22
PRIMER_LEFT_TM=60.190
PRIMER_RIGHT_TM=60.154
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=0.4868
PRIMER_LEFT_1_PENALTY=0.425548
PRIMER_RIGHT_1_PENALTY=0.061230
PRIMER_LEFT_1_SEQUENCE=GAAGCAAATATGCATGAGATGG
PRIMER_RIGHT_1_SEQUENCE=CATCTGATAAATCTTCCTCGGC
PRIMER_LEFT_1=219,22
PRIMER_RIGHT_1=441,22
PRIMER_LEFT_1_TM=59.574
PRIMER_RIGHT_1_TM=60.061
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.5019
PRIMER_LEFT_2_PENALTY=0.189596
PRIMER_RIGHT_2_PENALTY=0.312329
PRIMER_LEFT_2_SEQUENCE=TGTTGTGAAGCCTAGAGAAGCA
PRIMER_RIGHT_2_SEQUENCE=TCTCTTCCCTAGAAATGATGGC
PRIMER_LEFT_2=203,22
PRIMER_RIGHT_2=371,22
PRIMER_LEFT_2_TM=60.190
PRIMER_RIGHT_2_TM=59.688
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.5572
PRIMER_LEFT_3_PENALTY=0.425548
PRIMER_RIGHT_3_PENALTY=0.131699
PRIMER_LEFT_3_SEQUENCE=GAAGCAAATATGCATGAGATGG
PRIMER_RIGHT_3_SEQUENCE=TGTTGAGCCTCCTCTTCTTCTC
PRIMER_LEFT_3=219,22
PRIMER_RIGHT_3=480,22
PRIMER_LEFT_3_TM=59.574
PRIMER_RIGHT_3_TM=60.132
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=262
PRIMER_PAIR_PENALTY_4=0.5572
PRIMER_LEFT_4_PENALTY=0.425548
PRIMER_RIGHT_4_PENALTY=0.131699
PRIMER_LEFT_4_SEQUENCE=GAAGCAAATATGCATGAGATGG
PRIMER_RIGHT_4_SEQUENCE=TTGAGCCTCCTCTTCTTCTCAC
PRIMER_LEFT_4=219,22
PRIMER_RIGHT_4=478,22
PRIMER_LEFT_4_TM=59.574
PRIMER_RIGHT_4_TM=60.132
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=260
=
PRIMER_SEQUENCE_ID=SSR_05795 Ps_contig_mira-and-tgicl-ass_franssen_39957
SEQUENCE=AGAAAGCACACGTCACGACCCATGCTACTAAGGTAAGCTTTATTAGCATGAGTTTTCTGGCTTGTTAGAGCATTCCACACTATTAATCTTCCATCTTGGGATGCACTAACAATCCTATTCTTTTTCTGAAGTCCAATCCAATGAATACACCTTTCCGGTATGACCTTGGAGCGTTCTACAGCAAAGAATATCAGTGGGACCAAAAGTAACAGGAGCTCTACCTTGAGACTATCTGTATCAAGCAAAGAAAGCCGTCTCTGCTTCAATCGTTCTCTGAGATTGTTAACCGTTTCCGTCGCTGCTATGTGACGTTCTTTGAGCTCCGCAACGGACATTTTTTCAAGTGAATGGAAATTGAAGTGAATGAATTTGACCTAATAATGATATGAATCTAACAACTATTACTTTCCATTTATTTGTTTGTTTGTATTTTCAGAATAAGAAAACAAAGGTACGGTTTAGGTTAGGTTAAGGGGAAGAAAAGT
TARGET=416,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2131
PRIMER_LEFT_PENALTY=0.099308
PRIMER_RIGHT_PENALTY=0.113750
PRIMER_LEFT_SEQUENCE=GTGGGACCAAAAGTAACAGGAG
PRIMER_RIGHT_SEQUENCE=CCCCTTAACCTAACCTAAACCG
PRIMER_LEFT=193,22
PRIMER_RIGHT=475,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=60.114
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=283
PRIMER_PAIR_PENALTY_1=0.2131
PRIMER_LEFT_1_PENALTY=0.099308
PRIMER_RIGHT_1_PENALTY=0.113750
PRIMER_LEFT_1_SEQUENCE=AGTGGGACCAAAAGTAACAGGA
PRIMER_RIGHT_1_SEQUENCE=CCCCTTAACCTAACCTAAACCG
PRIMER_LEFT_1=192,22
PRIMER_RIGHT_1=475,22
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=60.114
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=0.2363
PRIMER_LEFT_2_PENALTY=0.122569
PRIMER_RIGHT_2_PENALTY=0.113750
PRIMER_LEFT_2_SEQUENCE=GAGATTGTTAACCGTTTCCGTC
PRIMER_RIGHT_2_SEQUENCE=CCCCTTAACCTAACCTAAACCG
PRIMER_LEFT_2=275,22
PRIMER_RIGHT_2=475,22
PRIMER_LEFT_2_TM=59.877
PRIMER_RIGHT_2_TM=60.114
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.3565
PRIMER_LEFT_3_PENALTY=0.242742
PRIMER_RIGHT_3_PENALTY=0.113750
PRIMER_LEFT_3_SEQUENCE=TCTCTGCTTCAATCGTTCTCTG
PRIMER_RIGHT_3_SEQUENCE=CCCCTTAACCTAACCTAAACCG
PRIMER_LEFT_3=254,22
PRIMER_RIGHT_3=475,22
PRIMER_LEFT_3_TM=59.757
PRIMER_RIGHT_3_TM=60.114
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.3565
PRIMER_LEFT_4_PENALTY=0.242742
PRIMER_RIGHT_4_PENALTY=0.113750
PRIMER_LEFT_4_SEQUENCE=CTCTGCTTCAATCGTTCTCTGA
PRIMER_RIGHT_4_SEQUENCE=CCCCTTAACCTAACCTAAACCG
PRIMER_LEFT_4=255,22
PRIMER_RIGHT_4=475,22
PRIMER_LEFT_4_TM=59.757
PRIMER_RIGHT_4_TM=60.114
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_05796 Ps_contig_mira-and-tgicl-ass_franssen_39963
SEQUENCE=TCCAGTCCGTCCTTATATCTTTCAGAAGCTTTTTCTTTTTTCTTTACTCTCTTTCTCACTTTGTTGGGATTCTTCAGCATGGTTTAGGTTTTTGTCTTTGCTTTGCTTTGCACCAGCAGATTTCAGTGCAGTCCATGTAAGATGCTTTTCGTTGTTTTGGTTAACTAAGTAATATACAGTTGTGGGATGACATGATCTTGCAGCCAAATGCAAAGGTGTATCTCCATTATTATCCTTTTGATTTATCAGCTTTTGATGTTCAGGAATGTCACTTTGTAATATGTAGCATACTATCTCATATTTTCCATGGTTTGCTGCAATGTGGAGAATATTACGCTTATGAGAAGTGTCAAGCATCTACTGTTGGATCTGGACAATATTGTAACAACTTCTTCACTACTTCCACATAGCCA
TARGET=96,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0377
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=GGATTCTTCAGCATGGTTTAGG
PRIMER_RIGHT_SEQUENCE=TGGAGATACACCTTTGCATTTG
PRIMER_LEFT=66,22
PRIMER_RIGHT=225,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.5000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=160
PRIMER_PAIR_PENALTY_1=0.0618
PRIMER_LEFT_1_PENALTY=0.034102
PRIMER_RIGHT_1_PENALTY=0.027715
PRIMER_LEFT_1_SEQUENCE=GGATTCTTCAGCATGGTTTAGG
PRIMER_RIGHT_1_SEQUENCE=AGATACACCTTTGCATTTGGCT
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=222,22
PRIMER_LEFT_1_TM=59.966
PRIMER_RIGHT_1_TM=60.028
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=0.1722
PRIMER_LEFT_2_PENALTY=0.034102
PRIMER_RIGHT_2_PENALTY=0.138114
PRIMER_LEFT_2_SEQUENCE=GGATTCTTCAGCATGGTTTAGG
PRIMER_RIGHT_2_SEQUENCE=TATTCTCCACATTGCAGCAAAC
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=330,22
PRIMER_LEFT_2_TM=59.966
PRIMER_RIGHT_2_TM=60.138
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.5000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.1835
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.149395
PRIMER_LEFT_3_SEQUENCE=GGATTCTTCAGCATGGTTTAGG
PRIMER_RIGHT_3_SEQUENCE=AGATCATGTCATCCCACAACTG
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=197,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.851
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=0.2746
PRIMER_LEFT_4_PENALTY=0.034102
PRIMER_RIGHT_4_PENALTY=0.240472
PRIMER_LEFT_4_SEQUENCE=GGATTCTTCAGCATGGTTTAGG
PRIMER_RIGHT_4_SEQUENCE=CAAGATCATGTCATCCCACAAC
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=59.966
PRIMER_RIGHT_4_TM=60.240
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_05797 Ps_contig_mira-and-tgicl-ass_franssen_39965
SEQUENCE=AGACAGAGAAAGAACCTCAAACCAAACTAAAGACTATTTTGGTTTATNTTTTCTAGTATGCTATATTCGGTATCGCAGACATGTTCACACTCGTAGGACTATTGGAATTCTTCTACAGAGAAGCACCTTCAACCATGAAATCACTTTCAACTTCATTCACATTTTTGTCCATGTCGCTCGGTTACTTCCTGAGTACTATTTTCGTTAACATTATTAATTCCGTTACGAATGGTATCAACGCAGAGTAAACAAGGTTGGTTACATGGTTTTGATTTCAATCAAAACAATCTTAACCTGTTCTATTGGTTCTTAGCAATATTGAGCTGTCTTAATTTCTTTAACTTCCTATATTGGGCCTCTTGGTACAAGTACAAAACTGAAGATAGTAACAATTCAGAGATGAACTCAAAGGAATTAGGTGAAACGCCTCTGTTAATGGTTGGTGGTGGAAGAAAACATGATGGTAAGGCCAAAGAAAGTAGCCAAACGAGTGAAGCCAATACTGAGGGACCCTCTTCTTCTGATGAAACAGATGATGGAAAAGAGAAAGAATGGAAGCATAGATAAAATTGTTGAAGATATGATTTGATGTTAAGGAAATAGTAGTAGTAGGCTGTGTATGGTAAAGATTGAATGTGAAAATAGAAAGTGGTTGAAATTTCTATTATCTTATATGTGTGATTGTATAATTGATTTGTACTATTGTTGGTTCTGGCACCATCTCTGTGTCTGTTCAGAGTCAAAGGTTTTCTCTTCTTAAACTT
TARGET=601,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9803
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.979555
PRIMER_LEFT_SEQUENCE=TGATGGTAAGGCCAAAGAAAGT
PRIMER_RIGHT_SEQUENCE=GGTGCCAGAACCAACAATAGTA
PRIMER_LEFT=458,22
PRIMER_RIGHT=718,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.020
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=4.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=1.0254
PRIMER_LEFT_1_PENALTY=0.045851
PRIMER_RIGHT_1_PENALTY=0.979555
PRIMER_LEFT_1_SEQUENCE=AAAGTAGCCAAACGAGTGAAGC
PRIMER_RIGHT_1_SEQUENCE=GGTGCCAGAACCAACAATAGTA
PRIMER_LEFT_1=475,22
PRIMER_RIGHT_1=718,22
PRIMER_LEFT_1_TM=59.954
PRIMER_RIGHT_1_TM=59.020
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=4.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=1.1444
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=1.143669
PRIMER_LEFT_2_SEQUENCE=TGATGGTAAGGCCAAAGAAAGT
PRIMER_RIGHT_2_SEQUENCE=ATGGTGCCAGAACCAACAATAG
PRIMER_LEFT_2=458,22
PRIMER_RIGHT_2=720,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=61.144
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=263
PRIMER_PAIR_PENALTY_3=1.1752
PRIMER_LEFT_3_PENALTY=0.000742
PRIMER_RIGHT_3_PENALTY=1.174455
PRIMER_LEFT_3_SEQUENCE=TGATGGTAAGGCCAAAGAAAGT
PRIMER_RIGHT_3_SEQUENCE=AACAGACACAGAGATGGTGCC
PRIMER_LEFT_3=458,22
PRIMER_RIGHT_3=733,21
PRIMER_LEFT_3_TM=60.001
PRIMER_RIGHT_3_TM=60.174
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=1.1752
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=1.174455
PRIMER_LEFT_4_SEQUENCE=TGATGGTAAGGCCAAAGAAAGT
PRIMER_RIGHT_4_SEQUENCE=ACACAGAGATGGTGCCAGAAC
PRIMER_LEFT_4=458,22
PRIMER_RIGHT_4=728,21
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=60.174
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_05798 Ps_contig_mira-and-tgicl-ass_franssen_39966
SEQUENCE=ATTGCGGGACCATTTTCCAAACCAATTATCCAATCCTTATGTAACCCACTTTTTCTTTAACCCAACCAACCAAACGCTTTGTCTTTTCCCTCAAAACGTCGTCGTCAACCTTTTCAAATTTCAAGAAAAGTGAAACAAACAAAAGCTTGAAAATCGATTCTCATTTCTAGGTTATTCATTCCTCTTTCTTCATATATTCTTTTTATTATTATTCTAATAATATTGAAATTTTAATTTATTTTTAATTTTCTCTTAGATTATATATTTTCCGTTTTTTCCCTCTGATTTTCGGAAGAAAAAAAAAGAAGAACATGATGAGGGGACATCAAGATCAACAATCGAAGATTATCTGTGAATTATCGTCTCTGGTTTTCAATCTTCTACAGCTTCCGCCGACGCCGTTAACTTTTTCCGATCGAGCTCCGGTCATGCCGGTGCCTCCGTCGGTGTCGTCGCTGAGGCGTTCGATGGCGGCGAATAAGATAACACCGGCGGGATTCGTTTCGTTGCTTTTGGGAATCGGGTAAGGGATAACCCTCGTTGGGATTAACGACGGAGTACTTGCCAACTGTCATGGAACTGCTCTTCATGTCCTCAGAGATGCATTTGGTTGCACCAGAT
TARGET=62,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.5663
PRIMER_LEFT_PENALTY=2.506395
PRIMER_RIGHT_PENALTY=0.059875
PRIMER_LEFT_SEQUENCE=TCCAATCCTTATGTAACCCACT
PRIMER_RIGHT_SEQUENCE=TCCCCTCATCATGTTCTTCTTT
PRIMER_LEFT=28,22
PRIMER_RIGHT=322,22
PRIMER_LEFT_TM=57.494
PRIMER_RIGHT_TM=59.940
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=2.7044
PRIMER_LEFT_1_PENALTY=2.506395
PRIMER_RIGHT_1_PENALTY=0.197963
PRIMER_LEFT_1_SEQUENCE=TCCAATCCTTATGTAACCCACT
PRIMER_RIGHT_1_SEQUENCE=ATGTCCCCTCATCATGTTCTTC
PRIMER_LEFT_1=28,22
PRIMER_RIGHT_1=325,22
PRIMER_LEFT_1_TM=57.494
PRIMER_RIGHT_1_TM=60.198
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=2.7044
PRIMER_LEFT_2_PENALTY=2.506395
PRIMER_RIGHT_2_PENALTY=0.197963
PRIMER_LEFT_2_SEQUENCE=TCCAATCCTTATGTAACCCACT
PRIMER_RIGHT_2_SEQUENCE=GATGTCCCCTCATCATGTTCTT
PRIMER_LEFT_2=28,22
PRIMER_RIGHT_2=326,22
PRIMER_LEFT_2_TM=57.494
PRIMER_RIGHT_2_TM=60.198
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=2.9828
PRIMER_LEFT_3_PENALTY=2.922893
PRIMER_RIGHT_3_PENALTY=0.059875
PRIMER_LEFT_3_SEQUENCE=CCAATCCTTATGTAACCCACTT
PRIMER_RIGHT_3_SEQUENCE=TCCCCTCATCATGTTCTTCTTT
PRIMER_LEFT_3=29,22
PRIMER_RIGHT_3=322,22
PRIMER_LEFT_3_TM=57.077
PRIMER_RIGHT_3_TM=59.940
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=3.0464
PRIMER_LEFT_4_PENALTY=2.506395
PRIMER_RIGHT_4_PENALTY=0.540037
PRIMER_LEFT_4_SEQUENCE=TCCAATCCTTATGTAACCCACT
PRIMER_RIGHT_4_SEQUENCE=CCCCTCATCATGTTCTTCTTTT
PRIMER_LEFT_4=28,22
PRIMER_RIGHT_4=321,22
PRIMER_LEFT_4_TM=57.494
PRIMER_RIGHT_4_TM=59.460
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=294
=
PRIMER_SEQUENCE_ID=SSR_05799 Ps_contig_mira-and-tgicl-ass_franssen_39971
SEQUENCE=TTTATAAACACCTCCATTTGAAAAACCGGTTCCGGTTTTATCAAACCGGCCATCCCCATCGAACCCACTCGCTACCGGTTTGAATTTCCCGCCTCGGCCGCTTCCTCCGTCGACCGAAGCCGTGTTATTTCCGTTTGATACAGAGAACGATTTAATCACAGACGAATCGAATTTCTGAAAGTCATTATCGTGAAACTCGACGATAGATTCCGTCTCAACCGGCGGAGCAACGGCGGCGATAGCGTTGTTGCTTGACATTGAATGAACTGTAAAATTTGCTAAGAGAATCATAACATAAACCATTAAAGTCGGTGTATGAGAGAAAACTTGTTGAAATAGCCACACGAATGAAGCGTGCATTTCCCTTTGTACCCTTTCGAGAATCCCTTGTAAATCCTCGTAGAGAAGAGCCTCTCTCATTTGTAAAGTGAAACATTGAAGCTCTCTGATGATGAAAACCATGGAAGAAAAAGCTTTCTTCACTGAACAACGCGCCGATTCACCTACCTCTCTGAACTCTTCTTTCAATCTCAGCTTCTTTTTTATAATCCGCAACGAAAACGGAAGCTCCACGCTGTTCGCTTTCCGTTCAATTATCGCCGGCATGATTTCCTCACCGCCATGGCTTCCGTTCCAAGGCGGTTCAAACTCTGCCGCAAAGTTAACCTTCGAAGAACCCGAAGAAGAAGAAGAAGCTAAACTTGTTTTTAAACTCTCTTCGAAACTTTCCAAATCCATAATCGCATTGTTCTCGCTTGAAATCTGGAACCTGAAAGCTAAATCTT
TARGET=680,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1412
PRIMER_LEFT_PENALTY=0.023913
PRIMER_RIGHT_PENALTY=0.117281
PRIMER_LEFT_SEQUENCE=GTTCGCTTTCCGTTCAATTATC
PRIMER_RIGHT_SEQUENCE=CAGATTTCAAGCGAGAACAATG
PRIMER_LEFT=576,22
PRIMER_RIGHT=764,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.883
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.2655
PRIMER_LEFT_1_PENALTY=0.023913
PRIMER_RIGHT_1_PENALTY=0.241544
PRIMER_LEFT_1_SEQUENCE=GTTCGCTTTCCGTTCAATTATC
PRIMER_RIGHT_1_SEQUENCE=AGCTTTCAGGTTCCAGATTTCA
PRIMER_LEFT_1=576,22
PRIMER_RIGHT_1=777,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=60.242
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.6166
PRIMER_LEFT_2_PENALTY=0.023913
PRIMER_RIGHT_2_PENALTY=0.592680
PRIMER_LEFT_2_SEQUENCE=GTTCGCTTTCCGTTCAATTATC
PRIMER_RIGHT_2_SEQUENCE=TTTCAGGTTCCAGATTTCAAGC
PRIMER_LEFT_2=576,22
PRIMER_RIGHT_2=774,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.593
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=199
PRIMER_PAIR_PENALTY_3=0.6166
PRIMER_LEFT_3_PENALTY=0.023913
PRIMER_RIGHT_3_PENALTY=0.592680
PRIMER_LEFT_3_SEQUENCE=GTTCGCTTTCCGTTCAATTATC
PRIMER_RIGHT_3_SEQUENCE=GCTTTCAGGTTCCAGATTTCAA
PRIMER_LEFT_3=576,22
PRIMER_RIGHT_3=776,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=60.593
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=201
PRIMER_PAIR_PENALTY_4=0.6361
PRIMER_LEFT_4_PENALTY=0.023913
PRIMER_RIGHT_4_PENALTY=0.612170
PRIMER_LEFT_4_SEQUENCE=GTTCGCTTTCCGTTCAATTATC
PRIMER_RIGHT_4_SEQUENCE=CCAGATTTCAAGCGAGAACAAT
PRIMER_LEFT_4=576,22
PRIMER_RIGHT_4=765,22
PRIMER_LEFT_4_TM=59.976
PRIMER_RIGHT_4_TM=60.612
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_05800 Ps_contig_mira-and-tgicl-ass_franssen_39972
SEQUENCE=TCAGAAACAAGAACATGCTACTGAAGAAGAAGCACAAGCCATCATTGATAGTTTCAAACATTTTCATCGAAGGGGCGCTGGACTTAACCTTGAAACTTTCTTCAAGTTTCTCTTCAGTGATAGTAACCTTTCTATTTTACCTTCAATTGGGGTATGTTGTAAATATATAAAAATATGATTTTTAATTTTTTCTTTCTTTGTTTTTTGTTTGTTTGTACCCTATGTTGTTGTTTTTTAGTTCTAAATGAATCTGGTATTTTGGTTTAAATTTTTCTAATTTGGGGTTGTTTCTTAGTTGGGTTTTGTTACCTTGTTTA
TARGET=204,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4225
PRIMER_LEFT_PENALTY=0.180440
PRIMER_RIGHT_PENALTY=0.242013
PRIMER_LEFT_SEQUENCE=CGCTGGACTTAACCTTGAAACT
PRIMER_RIGHT_SEQUENCE=CCAACTAAGAAACAACCCCAAA
PRIMER_LEFT=75,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=59.820
PRIMER_RIGHT_TM=60.242
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=224
PRIMER_PAIR_PENALTY_1=0.5116
PRIMER_LEFT_1_PENALTY=0.269585
PRIMER_RIGHT_1_PENALTY=0.242013
PRIMER_LEFT_1_SEQUENCE=AGAAGAAGCACAAGCCATCATT
PRIMER_RIGHT_1_SEQUENCE=CCAACTAAGAAACAACCCCAAA
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=298,22
PRIMER_LEFT_1_TM=60.270
PRIMER_RIGHT_1_TM=60.242
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.5116
PRIMER_LEFT_2_PENALTY=0.269585
PRIMER_RIGHT_2_PENALTY=0.242013
PRIMER_LEFT_2_SEQUENCE=AAGAAGAAGCACAAGCCATCAT
PRIMER_RIGHT_2_SEQUENCE=CCAACTAAGAAACAACCCCAAA
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=298,22
PRIMER_LEFT_2_TM=60.270
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.5000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.6568
PRIMER_LEFT_3_PENALTY=0.180440
PRIMER_RIGHT_3_PENALTY=0.476404
PRIMER_LEFT_3_SEQUENCE=CGCTGGACTTAACCTTGAAACT
PRIMER_RIGHT_3_SEQUENCE=AACCCAACTAAGAAACAACCCC
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=301,22
PRIMER_LEFT_3_TM=59.820
PRIMER_RIGHT_3_TM=60.476
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=10.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=227
PRIMER_PAIR_PENALTY_4=0.7460
PRIMER_LEFT_4_PENALTY=0.269585
PRIMER_RIGHT_4_PENALTY=0.476404
PRIMER_LEFT_4_SEQUENCE=AGAAGAAGCACAAGCCATCATT
PRIMER_RIGHT_4_SEQUENCE=AACCCAACTAAGAAACAACCCC
PRIMER_LEFT_4=24,22
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=60.270
PRIMER_RIGHT_4_TM=60.476
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=10.6000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_05801 Ps_contig_mira-and-tgicl-ass_franssen_39977
SEQUENCE=CCTGTTTAAACAGCTTACAGATGCCAGTCAACAATTTCGTGATGCTAACACAAATAACCGAGTTCTAAAATCAGATGTCGAAGCTTTGAGGGCTAAGGTGAAGTTAGCCGAGGATATGGTCTCTAGGGGCACTCTCCCCACTTTTAACAACCAGCTTCTCCAAAATCATAGTCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCACCGGTTTACAAATCCTCCCCCTCACCATACAAATATCAATCT
TARGET=179,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1040
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=0.090580
PRIMER_LEFT_SEQUENCE=GTGAAGTTAGCCGAGGATATGG
PRIMER_RIGHT_SEQUENCE=GATATTTGTATGGTGAGGGGGA
PRIMER_LEFT=97,22
PRIMER_RIGHT=262,22
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=59.909
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.1040
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=0.090580
PRIMER_LEFT_1_SEQUENCE=GGTGAAGTTAGCCGAGGATATG
PRIMER_RIGHT_1_SEQUENCE=GATATTTGTATGGTGAGGGGGA
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=262,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=59.909
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.1040
PRIMER_LEFT_2_PENALTY=0.013401
PRIMER_RIGHT_2_PENALTY=0.090580
PRIMER_LEFT_2_SEQUENCE=TGAAGTTAGCCGAGGATATGGT
PRIMER_RIGHT_2_SEQUENCE=GATATTTGTATGGTGAGGGGGA
PRIMER_LEFT_2=98,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=59.987
PRIMER_RIGHT_2_TM=59.909
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.1256
PRIMER_LEFT_3_PENALTY=0.035021
PRIMER_RIGHT_3_PENALTY=0.090580
PRIMER_LEFT_3_SEQUENCE=CCCACTTTTAACAACCAGCTTC
PRIMER_RIGHT_3_SEQUENCE=GATATTTGTATGGTGAGGGGGA
PRIMER_LEFT_3=136,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=59.909
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=0.1480
PRIMER_LEFT_4_PENALTY=0.057411
PRIMER_RIGHT_4_PENALTY=0.090580
PRIMER_LEFT_4_SEQUENCE=ACAGCTTACAGATGCCAGTCAA
PRIMER_RIGHT_4_SEQUENCE=GATATTTGTATGGTGAGGGGGA
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=262,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=59.909
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_05802 Ps_contig_mira-and-tgicl-ass_franssen_39977
SEQUENCE=CCTGTTTAAACAGCTTACAGATGCCAGTCAACAATTTCGTGATGCTAACACAAATAACCGAGTTCTAAAATCAGATGTCGAAGCTTTGAGGGCTAAGGTGAAGTTAGCCGAGGATATGGTCTCTAGGGGCACTCTCCCCACTTTTAACAACCAGCTTCTCCAAAATCATAGTCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCACCGGTTTACAAATCCTCCCCCTCACCATACAAATATCAATCT
TARGET=216,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1040
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=0.090580
PRIMER_LEFT_SEQUENCE=GTGAAGTTAGCCGAGGATATGG
PRIMER_RIGHT_SEQUENCE=GATATTTGTATGGTGAGGGGGA
PRIMER_LEFT=97,22
PRIMER_RIGHT=262,22
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=59.909
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.1040
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=0.090580
PRIMER_LEFT_1_SEQUENCE=GGTGAAGTTAGCCGAGGATATG
PRIMER_RIGHT_1_SEQUENCE=GATATTTGTATGGTGAGGGGGA
PRIMER_LEFT_1=96,22
PRIMER_RIGHT_1=262,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=59.909
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.1040
PRIMER_LEFT_2_PENALTY=0.013401
PRIMER_RIGHT_2_PENALTY=0.090580
PRIMER_LEFT_2_SEQUENCE=TGAAGTTAGCCGAGGATATGGT
PRIMER_RIGHT_2_SEQUENCE=GATATTTGTATGGTGAGGGGGA
PRIMER_LEFT_2=98,22
PRIMER_RIGHT_2=262,22
PRIMER_LEFT_2_TM=59.987
PRIMER_RIGHT_2_TM=59.909
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.8000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.1256
PRIMER_LEFT_3_PENALTY=0.035021
PRIMER_RIGHT_3_PENALTY=0.090580
PRIMER_LEFT_3_SEQUENCE=CCCACTTTTAACAACCAGCTTC
PRIMER_RIGHT_3_SEQUENCE=GATATTTGTATGGTGAGGGGGA
PRIMER_LEFT_3=136,22
PRIMER_RIGHT_3=262,22
PRIMER_LEFT_3_TM=60.035
PRIMER_RIGHT_3_TM=59.909
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=10.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=0.1480
PRIMER_LEFT_4_PENALTY=0.057411
PRIMER_RIGHT_4_PENALTY=0.090580
PRIMER_LEFT_4_SEQUENCE=ACAGCTTACAGATGCCAGTCAA
PRIMER_RIGHT_4_SEQUENCE=GATATTTGTATGGTGAGGGGGA
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=262,22
PRIMER_LEFT_4_TM=59.943
PRIMER_RIGHT_4_TM=59.909
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_05803 Ps_contig_mira-and-tgicl-ass_franssen_39987
SEQUENCE=ATGGTTGTTACGGTTGCAGAGTNAAGGGTNNCAGAACAGAATGAAGAGATAAGAGAAAGAGTGAAGAGAAAGATTAGAAAAGTGAAGAAACTCGTGGCTCGAGCCAATGGTGGAGAACTGACCTAATTTATAGTAGTTTCTATGAAGTGTATTTCGTTAACTCTATTATCTCTCNCCACTAATTNTACGTNAACTCTTAGTTAGTTTAGTTTAGTTTAGTTAATTGTATTTATTACTTAAGTTTAAAATTTC
TARGET=201,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05804 Ps_contig_mira-and-tgicl-ass_franssen_39988
SEQUENCE=AAGCAGTTGGTATCAACGCAGAGTTGGACTATCTTGCAACTCTAAAACCCAAACCTGAAATGACTGAAGATGATAATAACCCGGCTGAAAATATCGTTGTGGAAGAAGATGGGAAAATTTGCATTACTAGTACTGTCCTCACTAGCCCCGGAACAAGCGAGGTTGTTATTTTTGCTGTACTGTATTGTAAACTTAGAAAAATTCCAAACTCAAATGGTGGTAACCAAGAGAAATATGCAGCAAGGATTCTTGATTTTTTGAGCTCTAAGAGATCTGGTAAAAGAGAGAGAGATTCATGGAATACATTTCTATTGCAATCTAAAGGAGATTTAAGAGATCTTATTTTCACAAAACTGGTACACGTCAGAGTAAGATTAAATTGTTTTGATCATCTTAAGACTGATAATGAAAAGGATTTCATCTTAACAGATTCCTCAATTAAAGTGAATGTGTTGCTCAAGTGACAGTTAGATTTTAGGAACACGTTAGTGTCCTGTGTTTTATTCTTTTGAATGTATAATCAAAGTTGGTATTTAGCAACTTTTTGCACCATTGTTTCAAGAGTAGTAGCTTAAAAATAGTTCTTGTGAAAGATCTTTTGTTTGTTTTGAATAAAATTGATTTTTCTTCTGGAATTTCTGTACCAAGGATATTTATTTGTATAAGAACTTGAGTGTCTTGTGAAACGAAATCTGAAATGGTCTCTGTAGTATTTGGAAACAAGTTGTAATATTTGGACATATACTGATATACAGCGTTATTCATCCNANCCAAT
TARGET=282,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8508
PRIMER_LEFT_PENALTY=0.503688
PRIMER_RIGHT_PENALTY=0.347100
PRIMER_LEFT_SEQUENCE=CAAGAGAAATATGCAGCAAGGA
PRIMER_RIGHT_SEQUENCE=TGTCACTTGAGCAACACATTCA
PRIMER_LEFT=224,22
PRIMER_RIGHT=465,22
PRIMER_LEFT_TM=59.496
PRIMER_RIGHT_TM=60.347
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=1.0243
PRIMER_LEFT_1_PENALTY=0.168187
PRIMER_RIGHT_1_PENALTY=0.856103
PRIMER_LEFT_1_SEQUENCE=GATGATAATAACCCGGCTGAAA
PRIMER_RIGHT_1_SEQUENCE=TGACGTGTACCAGTTTTGTGAA
PRIMER_LEFT_1=68,22
PRIMER_RIGHT_1=365,22
PRIMER_LEFT_1_TM=60.168
PRIMER_RIGHT_1_TM=59.144
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=1.0273
PRIMER_LEFT_2_PENALTY=0.171245
PRIMER_RIGHT_2_PENALTY=0.856103
PRIMER_LEFT_2_SEQUENCE=AGATGATAATAACCCGGCTGAA
PRIMER_RIGHT_2_SEQUENCE=TGACGTGTACCAGTTTTGTGAA
PRIMER_LEFT_2=67,22
PRIMER_RIGHT_2=365,22
PRIMER_LEFT_2_TM=59.829
PRIMER_RIGHT_2_TM=59.144
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=299
PRIMER_PAIR_PENALTY_3=1.1882
PRIMER_LEFT_3_PENALTY=0.332137
PRIMER_RIGHT_3_PENALTY=0.856103
PRIMER_LEFT_3_SEQUENCE=GTTGTGGAAGAAGATGGGAAAA
PRIMER_RIGHT_3_SEQUENCE=TGACGTGTACCAGTTTTGTGAA
PRIMER_LEFT_3=95,22
PRIMER_RIGHT_3=365,22
PRIMER_LEFT_3_TM=60.332
PRIMER_RIGHT_3_TM=59.144
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=271
PRIMER_PAIR_PENALTY_4=1.2853
PRIMER_LEFT_4_PENALTY=0.168187
PRIMER_RIGHT_4_PENALTY=1.117097
PRIMER_LEFT_4_SEQUENCE=GATGATAATAACCCGGCTGAAA
PRIMER_RIGHT_4_SEQUENCE=CTGACGTGTACCAGTTTTGTGAA
PRIMER_LEFT_4=68,22
PRIMER_RIGHT_4=366,23
PRIMER_LEFT_4_TM=60.168
PRIMER_RIGHT_4_TM=60.117
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_05805 Ps_contig_mira-and-tgicl-ass_franssen_39993
SEQUENCE=AATTTAANTGTATTATTATTACTATATCTGAAAAATAACCATTCACTCATTCTTGCAAGAAAATTAAAAACCATTCACTCATTTATGTATTATTATTACTATATCTGAAAAATAACCATTCACCCATTCTTGCAGAAATTAAAAACATCAACACAGTTTGAATTTAAAACAGTACAGAGAAGAAAAAAAAGTACCTTCACATTAACAAAACTAGATTGAATACCCATGAATTTCTATTTCAGATTATGAATGACTTTGGCTCATTCTGCCATCTTATCTTCTTCCTCCTTCTCATCATCATCATCATCATCATCTCTCAGTTCAAGATCATCAAGCTCAGCCAGACAACTCATTGAATGGAAACAGGAAGGCAGTCTCTTCACCATCAAAAGCAACAGATGTCCG
TARGET=292,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1191
PRIMER_LEFT_PENALTY=0.081801
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=ATAACCATTCACCCATTCTTGC
PRIMER_RIGHT_SEQUENCE=ACATCTGTTGCTTTTGATGGTG
PRIMER_LEFT=111,22
PRIMER_RIGHT=401,22
PRIMER_LEFT_TM=60.082
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.1591
PRIMER_LEFT_1_PENALTY=0.081801
PRIMER_RIGHT_1_PENALTY=0.077340
PRIMER_LEFT_1_SEQUENCE=ATAACCATTCACCCATTCTTGC
PRIMER_RIGHT_1_SEQUENCE=GTGAAGAGACTGCCTTCCTGTT
PRIMER_LEFT_1=111,22
PRIMER_RIGHT_1=382,22
PRIMER_LEFT_1_TM=60.082
PRIMER_RIGHT_1_TM=59.923
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.2862
PRIMER_LEFT_2_PENALTY=0.248900
PRIMER_RIGHT_2_PENALTY=0.037339
PRIMER_LEFT_2_SEQUENCE=TGAATGACTTTGGCTCATTCTG
PRIMER_RIGHT_2_SEQUENCE=ACATCTGTTGCTTTTGATGGTG
PRIMER_LEFT_2=246,22
PRIMER_RIGHT_2=401,22
PRIMER_LEFT_2_TM=60.249
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=156
PRIMER_PAIR_PENALTY_3=0.3225
PRIMER_LEFT_3_PENALTY=0.285163
PRIMER_RIGHT_3_PENALTY=0.037339
PRIMER_LEFT_3_SEQUENCE=GGCTCATTCTGCCATCTTATCT
PRIMER_RIGHT_3_SEQUENCE=ACATCTGTTGCTTTTGATGGTG
PRIMER_LEFT_3=257,22
PRIMER_RIGHT_3=401,22
PRIMER_LEFT_3_TM=59.715
PRIMER_RIGHT_3_TM=60.037
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=0.3262
PRIMER_LEFT_4_PENALTY=0.248900
PRIMER_RIGHT_4_PENALTY=0.077340
PRIMER_LEFT_4_SEQUENCE=TGAATGACTTTGGCTCATTCTG
PRIMER_RIGHT_4_SEQUENCE=GTGAAGAGACTGCCTTCCTGTT
PRIMER_LEFT_4=246,22
PRIMER_RIGHT_4=382,22
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=59.923
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_05806 Ps_contig_mira-and-tgicl-ass_franssen_39994
SEQUENCE=AGCATTCATCTTTTATGAGAACTTTACTGCTCTCATAAACTATGCTCCACATACTTACAATGACAAAATCATCTCGGGTCCGAAGAAAATCTCGGACATGTTTCCTTTTTTTCAAAGGAACCGTGTTTGAAAACGGGATTGACGCTGCATTTCTAGGTCAACTAAGGAGAAAGAAGTTTATTTATTCAAAGGAGACTTGTATGCTCGTATAGACTATGGAAAAAACTATCTGGTTCAAAGTATCAAGAACATTAGCACTGGGTTCCCTTGTTTCACTGGAACCGTCTTTGAAAATGGAGTGGATGCTGCTTTTGTCTTCTCACAGGACCAATGAAGCATTACTTTTTACAAAGGAGATTACTATGCACTTGTCAAGATTAGCCCGGGCGGAATAGATGACTATATTATCGGTGGTGTGAAGCCCATTCTTGAGAATTGGCCTTCTCTTCGTGGTATAATACCTCAGAAAAGTTAAATGTGGCTCTCTGTGTGTGTGTGATATCATCATGCAAGTATGGTATTAAGAATAAAGACTATTGTTGTCGTTGTTGTGTGTTTCTTTTTCATGTTGTTTCTAGTTCTTAATGTTTGCTTATGTTGTTCATGTGAACTATGTAATGACATGCACTGTG
TARGET=487,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5899
PRIMER_LEFT_PENALTY=0.148509
PRIMER_RIGHT_PENALTY=0.441361
PRIMER_LEFT_SEQUENCE=TTGTCTTCTCACAGGACCAATG
PRIMER_RIGHT_SEQUENCE=GAAACACACAACAACGACAACA
PRIMER_LEFT=311,22
PRIMER_RIGHT=558,22
PRIMER_LEFT_TM=60.149
PRIMER_RIGHT_TM=59.559
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.7161
PRIMER_LEFT_1_PENALTY=0.274705
PRIMER_RIGHT_1_PENALTY=0.441361
PRIMER_LEFT_1_SEQUENCE=CATTCTTGAGAATTGGCCTTCT
PRIMER_RIGHT_1_SEQUENCE=GAAACACACAACAACGACAACA
PRIMER_LEFT_1=423,22
PRIMER_RIGHT_1=558,22
PRIMER_LEFT_1_TM=59.725
PRIMER_RIGHT_1_TM=59.559
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=136
PRIMER_PAIR_PENALTY_2=0.7303
PRIMER_LEFT_2_PENALTY=0.288917
PRIMER_RIGHT_2_PENALTY=0.441361
PRIMER_LEFT_2_SEQUENCE=GCTTTTGTCTTCTCACAGGACC
PRIMER_RIGHT_2_SEQUENCE=GAAACACACAACAACGACAACA
PRIMER_LEFT_2=307,22
PRIMER_RIGHT_2=558,22
PRIMER_LEFT_2_TM=60.289
PRIMER_RIGHT_2_TM=59.559
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=0.7850
PRIMER_LEFT_3_PENALTY=0.343680
PRIMER_RIGHT_3_PENALTY=0.441361
PRIMER_LEFT_3_SEQUENCE=CTGCTTTTGTCTTCTCACAGGA
PRIMER_RIGHT_3_SEQUENCE=GAAACACACAACAACGACAACA
PRIMER_LEFT_3=305,22
PRIMER_RIGHT_3=558,22
PRIMER_LEFT_3_TM=59.656
PRIMER_RIGHT_3_TM=59.559
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.7938
PRIMER_LEFT_4_PENALTY=0.352395
PRIMER_RIGHT_4_PENALTY=0.441361
PRIMER_LEFT_4_SEQUENCE=CGTCTTTGAAAATGGAGTGGAT
PRIMER_RIGHT_4_SEQUENCE=GAAACACACAACAACGACAACA
PRIMER_LEFT_4=282,22
PRIMER_RIGHT_4=558,22
PRIMER_LEFT_4_TM=60.352
PRIMER_RIGHT_4_TM=59.559
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=277
=
PRIMER_SEQUENCE_ID=SSR_05807 Ps_contig_mira-and-tgicl-ass_franssen_40000
SEQUENCE=ACTTTAGTTATCAAATACGATGTCTGTATTTGTATTTTTTTAAATTAATAATACAATACAATCATTAATACTATACAATATGTAAAGTCTTAACTTTCTTAAAGTTTGTTCTTATAGTTTTCACTTGTGTCCCTCTCATGACCCCTCATTCACTCCCATACCCATCTCTCCAATCCCTTTTAGAACATATATATATAAAGTCTATCTATTAAAACRACTCAATTCATCAACCACCCTTTACCTTTAAAGGGGTTTTCAAGGTTGGTCCTAAAATGCTCTCAAAGGTTACTATTACTAGTACTACTATTACATACATATCCTCATTAATATTCTTCTCACACCCCTTCGACAAAAGGAGCAATGAATGGTCCAAGAAAGTCCATAGACAAATAAAATAAAAAAACTAAGAAAAGGAGGATATTTTTTTTGAGTAGTGGAATTAATCTCTTTTGGATATAATTTTAGCCATGTCGAATACCCATGAAATAATTGAAGTCATGATGTTGAACCCTTAGTTGCTTTAACCATGAGTCTGCCACACTTAATAGTGACATAAGTCTTTCTTGGTCCCGACCACGATTTCCTCCTGCCCACACATTCCCACCTTGCATCTTGTATGTTGCCAATCCAAACGCTGGCAATGACATGTTTTCTCCTTCCTTTTTCTTTTCTTTTCCACCTTCAATATCATCATCAAGGTCCATCCCTTGAAATGAAGATGAAAGTGTGTGGTAAGTAAGGAAGCATGTGGAAAGATCTTTAATTGTTCTTCCCATGGGAATATGATAAATTGGGTACCAAGCAACAGCCATCCAACTTGCTGGTGAAAGATCCACACTTCTTAGTGACATCAACCCAGGGTATCTTTGGGCTAATCCAGATATCTTATCCATTAGAGGTACTCTTCCATATGGATTTGATCTTTACAAAATTATTGAACAAATAAAGNTCGAACCCAATCTATCATTCAAAT
TARGET=187,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05808 Ps_contig_mira-and-tgicl-ass_franssen_40000
SEQUENCE=ACTTTAGTTATCAAATACGATGTCTGTATTTGTATTTTTTTAAATTAATAATACAATACAATCATTAATACTATACAATATGTAAAGTCTTAACTTTCTTAAAGTTTGTTCTTATAGTTTTCACTTGTGTCCCTCTCATGACCCCTCATTCACTCCCATACCCATCTCTCCAATCCCTTTTAGAACATATATATATAAAGTCTATCTATTAAAACRACTCAATTCATCAACCACCCTTTACCTTTAAAGGGGTTTTCAAGGTTGGTCCTAAAATGCTCTCAAAGGTTACTATTACTAGTACTACTATTACATACATATCCTCATTAATATTCTTCTCACACCCCTTCGACAAAAGGAGCAATGAATGGTCCAAGAAAGTCCATAGACAAATAAAATAAAAAAACTAAGAAAAGGAGGATATTTTTTTTGAGTAGTGGAATTAATCTCTTTTGGATATAATTTTAGCCATGTCGAATACCCATGAAATAATTGAAGTCATGATGTTGAACCCTTAGTTGCTTTAACCATGAGTCTGCCACACTTAATAGTGACATAAGTCTTTCTTGGTCCCGACCACGATTTCCTCCTGCCCACACATTCCCACCTTGCATCTTGTATGTTGCCAATCCAAACGCTGGCAATGACATGTTTTCTCCTTCCTTTTTCTTTTCTTTTCCACCTTCAATATCATCATCAAGGTCCATCCCTTGAAATGAAGATGAAAGTGTGTGGTAAGTAAGGAAGCATGTGGAAAGATCTTTAATTGTTCTTCCCATGGGAATATGATAAATTGGGTACCAAGCAACAGCCATCCAACTTGCTGGTGAAAGATCCACACTTCTTAGTGACATCAACCCAGGGTATCTTTGGGCTAATCCAGATATCTTATCCATTAGAGGTACTCTTCCATATGGATTTGATCTTTACAAAATTATTGAACAAATAAAGNTCGAACCCAATCTATCATTCAAAT
TARGET=662,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05809 Ps_contig_mira-and-tgicl-ass_franssen_40001
SEQUENCE=TGAAACCNGATCCCCGGACCACCGCCTCTATGCTCATGCTCTGGTATGTACACAATAATTGGCTGCTTACATAACTTTGATAATACCAATAGTTCCGATTCTCCTCCCCAGAAATCTGGTTGCACAATCCGCCTGCAGTAACGTTGTAAAGGCTCATCAACTGTGACAGCAATGATGGCTTCTTCATATAATTTCCGATCTCCTACATTTTCACATATAGCTTCTTTGACTGCCATTCTTAATTCATCTGCATTCTCTCTCTCCTCGCGTTGGTTAAGAGCAACTCCTTTGTTGTAAGCCATTCCTTTCACGAGCGCACGAAACAGACACCGCCCATCCCCTGTAACCTTGTGAACAGAAAAACGTTCAAGTTTCTTCACTGCTGCCGACTGTCGACCAATGGATGAANCAATTCTAGCGAAAAAGAGAGTGCTAGAACTTCCAGCTCCGAAGAAGCTTGAATTGGGAAGTTAAAAGGGGGGAAAACAAACGGGAAGGGGAACG
TARGET=254,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1941
PRIMER_LEFT_PENALTY=0.193337
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=CGTTGTAAAGGCTCATCAACTG
PRIMER_RIGHT_SEQUENCE=GGAATGGCTTACAACAAAGGAG
PRIMER_LEFT=141,22
PRIMER_RIGHT=304,22
PRIMER_LEFT_TM=59.807
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.1941
PRIMER_LEFT_1_PENALTY=0.193337
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=CGTTGTAAAGGCTCATCAACTG
PRIMER_RIGHT_1_SEQUENCE=AGGAATGGCTTACAACAAAGGA
PRIMER_LEFT_1=141,22
PRIMER_RIGHT_1=305,22
PRIMER_LEFT_1_TM=59.807
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=0.2311
PRIMER_LEFT_2_PENALTY=0.230329
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=CAGCAATGATGGCTTCTTCATA
PRIMER_RIGHT_2_SEQUENCE=AGGAATGGCTTACAACAAAGGA
PRIMER_LEFT_2=166,22
PRIMER_RIGHT_2=305,22
PRIMER_LEFT_2_TM=60.230
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=140
PRIMER_PAIR_PENALTY_3=0.2311
PRIMER_LEFT_3_PENALTY=0.230329
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=CAGCAATGATGGCTTCTTCATA
PRIMER_RIGHT_3_SEQUENCE=GGAATGGCTTACAACAAAGGAG
PRIMER_LEFT_3=166,22
PRIMER_RIGHT_3=304,22
PRIMER_LEFT_3_TM=60.230
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=0.3188
PRIMER_LEFT_4_PENALTY=0.230329
PRIMER_RIGHT_4_PENALTY=0.088479
PRIMER_LEFT_4_SEQUENCE=CAGCAATGATGGCTTCTTCATA
PRIMER_RIGHT_4_SEQUENCE=CAGCAGTGAAGAAACTTGAACG
PRIMER_LEFT_4=166,22
PRIMER_RIGHT_4=384,22
PRIMER_LEFT_4_TM=60.230
PRIMER_RIGHT_4_TM=60.088
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_05810 Ps_contig_mira-and-tgicl-ass_franssen_40005
SEQUENCE=CCTTTTCCTTCCCTTTCCTTTTTACTTTTCTTTCCTTGTTACCGTCCTTGTCAATGATGTGTGAGTTGACGTGGTTGCAGTGGATATTGGATTGACACAGGTGCGATAGCTATTGGTGACGATGTACGGCGAAGAAGATGGCGGTGAATCTTGTTATGCAGTGAAGAAGAGGAAGAATGAAATGGTGGCTTCCGTGCTTCTGCTTGCAATGCTTTGAGGAATCGATGATGGTAGATTCGTTGTGGACAGGTTGATGAAGCGTGAAGAATGAAGGTGAAGGTTTCGGTGAACGGAGATTACGGTTACGGTGGTTGAAGGTTTTTGGTTGAAGAATTGGAAATGGAGGTTTTGATGAAGGTTGCAGTTGAAGGATTGGGAGAGTTTGTTACAGTTTGGATGGAGAGAAGAAGAAGAAGAATCTGGAAGAAAAAATTTGAGAGCGGGAAGAAGGAGAATTTATA
TARGET=403,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3900
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.385604
PRIMER_LEFT_SEQUENCE=ATGAAGGTTGCAGTTGAAGGAT
PRIMER_RIGHT_SEQUENCE=ATAAATTCTCCTTCTTCCCGCT
PRIMER_LEFT=351,22
PRIMER_RIGHT=459,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.614
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.8000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=0.4159
PRIMER_LEFT_1_PENALTY=0.030276
PRIMER_RIGHT_1_PENALTY=0.385604
PRIMER_LEFT_1_SEQUENCE=TAGATTCGTTGTGGACAGGTTG
PRIMER_RIGHT_1_SEQUENCE=ATAAATTCTCCTTCTTCCCGCT
PRIMER_LEFT_1=231,22
PRIMER_RIGHT_1=459,22
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=59.614
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=229
PRIMER_PAIR_PENALTY_2=0.4255
PRIMER_LEFT_2_PENALTY=0.039879
PRIMER_RIGHT_2_PENALTY=0.385604
PRIMER_LEFT_2_SEQUENCE=AGGTTTTGATGAAGGTTGCAGT
PRIMER_RIGHT_2_SEQUENCE=ATAAATTCTCCTTCTTCCCGCT
PRIMER_LEFT_2=343,22
PRIMER_RIGHT_2=459,22
PRIMER_LEFT_2_TM=60.040
PRIMER_RIGHT_2_TM=59.614
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=11.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=0.4994
PRIMER_LEFT_3_PENALTY=0.113803
PRIMER_RIGHT_3_PENALTY=0.385604
PRIMER_LEFT_3_SEQUENCE=GGTTGAAGGTTTTTGGTTGAAG
PRIMER_RIGHT_3_SEQUENCE=ATAAATTCTCCTTCTTCCCGCT
PRIMER_LEFT_3=309,22
PRIMER_RIGHT_3=459,22
PRIMER_LEFT_3_TM=59.886
PRIMER_RIGHT_3_TM=59.614
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=11.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.5264
PRIMER_LEFT_4_PENALTY=0.140832
PRIMER_RIGHT_4_PENALTY=0.385604
PRIMER_LEFT_4_SEQUENCE=TGATGGTAGATTCGTTGTGGAC
PRIMER_RIGHT_4_SEQUENCE=ATAAATTCTCCTTCTTCCCGCT
PRIMER_LEFT_4=225,22
PRIMER_RIGHT_4=459,22
PRIMER_LEFT_4_TM=59.859
PRIMER_RIGHT_4_TM=59.614
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=11.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_05811 Ps_contig_mira-and-tgicl-ass_franssen_40010
SEQUENCE=TAAAGGTTAATGTTAGTTAGTTTATTGTATGCTACTGATGCATTGGAATTNTAGCTTGGATRTGTTGTTTTTGTCAAGTCTGATATGTTATGCCCAGATTGCAGGTTTGATGCAGGGTTTTGGCTTAATTTTATTGTGATGCAAGTCTTGGTAATTGCTTGGTCTATGTTGCCATGGTGATGGCACTTTTGATGGTTTGTTCGATGCAAGTGTGCAGGGTGCTAAGTGTCATGGCAAGATGCAAGTGTGCAGGGTGCTAAGTGTCATGGCAAGTTAGAGGGATGGTACTTGATAGATCTGGCCTTGCAGCAGGTCTATGATGCAAGTTTGGCTCATGACATGTTTGGTATTGCAACAGGTTTTGGTGCTTGATGATGATGAATTGCATATGTATGTTATGGTTCCATGGAGGCTTATGGCATGATGGTTGAACAACCAAGGTTACATGACACTCAATTTTGGATTATGAAGCATGATATGAAAGAGGAAATCCAATGAATCATGATGGAACTTGAAGATTAGGGTTAGGATCGGGATTTGGGATTTGGGATTTTTGGAATTGGGTTGACTTTGGTCAAAGTTAACCAAATGTAAACTGTTGACCAAAGTCAACATTTGGTCAACACTTCATTTTTTGTATTTTATTCATGTATGGACATTTTTATTGATTAATGTAATGGAATTTGATATTTGAATGNAGTGGATTGGTATTTAAATG
TARGET=370,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05812 Ps_contig_mira-and-tgicl-ass_franssen_40026
SEQUENCE=AGGGACCTTTCTTTCTTTCTTCTTCTCTTCACACTTGATTCATTTCATTCACACATCAACCAACAACAACGCACTTTCATTCACTTTTTCTCCCCTAACAACTAACCGTCTTTGTTTCTATCTGAAAATCAAACAACAATAATGGAAAGTATAGTTGTAGATTCATATCATTATTAGGTCAAATTCATTCACTTCAATTTCCATTCACTTGAAAAAATGTCCGTTGCGGAGCTCAAAGAACGTCACATAGCAGCGACGGAAACGGTTAACAATCTCAGAGAACGATTGAAGCAGAGACGGCTTTCTTTGCTTGATACAGATATTGCTGGATATGCTAGGTCTCAAGGTAGAGCTCCTGTTACTTTTGGTCCCACTGATATTCTTTGCTGTAGAACGCTCCAAGGTCATACCGGAAAGGTGTATTCATTGGATTGGACTTCAGAAAAGAATAGGATTGTTAGTGCATCCCAAGATGGAAGATTAATAGTGTGGAATGCTCTAACAAGCCAGAAAACTCATGCTATAAAGCTTCCTTGTGCATGGGTCATGACGTGTGCTTTCTCACCAACTGGTCAATCTGTTGCTTGTGGGGGCCTTGACAGTGTTTGCTCTATTTTCAATCTTAATTCTCCCACTGATAGGGATGGGAATCTAAATGTTTCACGGATGCTTAGTGGACATAAAGGTTATGTTTCATCTTGTCAGTATGTTCCAGGTGAAGACACTCACTTAATCACTGGTTCTGGAGATCAGACATGTGTTTTATGGGATATTACTACTGGCCTTAGAACATCTGTTTTTGGAGGCGAGTTTCAGTCTGGACATACTGCAGATGTACTTAGCATTTCCATTAATGGATCCAACTCCAAATTGTTTGTATCTGGTTCTTGCGATGCGACTGCCAGATTGTGGGACACTCGTGTGGCAAGTCGAGCAGTGCGGACATTTCACGGCCACGAGGGAGATGTTAATTCTGTCAAATTCTTTCCTGATGGAAATAGATTTGGAACTGGCTCAGAGGATGGAACTTGCAGATTATTTGACATTAGGACCGGACACCAACTTCAAGTATATAATCAGCAACACCAAGACAACGAAATGGCACATGTGACGTCCATTGCATTTTCCATATCCGGAAGACTTCTTATTGCTGGCTATACAAATGGTGATTGCTATGTTTGGGATACTTTATTGGCTAAGGTGGTCTTGAATCTAGGATCTCTTCAAAACTCTCATGAGGGCAGGATCACCTGTTTGGGTATGTCTGCTGATGGAAGCGCTTTATGTACAGGAAGTTGGGACACAAATTTAAAGATATGGGCATTTGGAGGGCATAGGAAGGTGATTTGACTCCATTGTTAGGGCTTCACCTTGTAATGATGCTTGTGATATTGACTTTGATCCAGAATTGGAAGGCAAAGTTTATCTCCATGTTTATAACCTTTAGCAGTGGAACTAGTGCAGCCTTTATTTATCTCCATGCTCATTGGTTCGTGTGTGTGATTTAGGTATATATATACCTTTAAACCAAAACAGAGGACTATTTAATTTTCTGTCTCCTCAATTTAACTATTTGAAGTATGTGTTTGGTTCACATTGGAAGAACTAAATGTACTAGTATGTTTATAGTGGTTGAATCAGATTTGGATCAGGTAAGGGGGTGTTTGGATCCCCATTGTAAAATCGAATCTTCTTCCTGTTGAAGCAACTCTTGTGTAACTTTTGGAAACCAAAAAAAAAANCTACATTAAAAACCTTTCATAACC
TARGET=7,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05813 Ps_contig_mira-and-tgicl-ass_franssen_40026
SEQUENCE=AGGGACCTTTCTTTCTTTCTTCTTCTCTTCACACTTGATTCATTTCATTCACACATCAACCAACAACAACGCACTTTCATTCACTTTTTCTCCCCTAACAACTAACCGTCTTTGTTTCTATCTGAAAATCAAACAACAATAATGGAAAGTATAGTTGTAGATTCATATCATTATTAGGTCAAATTCATTCACTTCAATTTCCATTCACTTGAAAAAATGTCCGTTGCGGAGCTCAAAGAACGTCACATAGCAGCGACGGAAACGGTTAACAATCTCAGAGAACGATTGAAGCAGAGACGGCTTTCTTTGCTTGATACAGATATTGCTGGATATGCTAGGTCTCAAGGTAGAGCTCCTGTTACTTTTGGTCCCACTGATATTCTTTGCTGTAGAACGCTCCAAGGTCATACCGGAAAGGTGTATTCATTGGATTGGACTTCAGAAAAGAATAGGATTGTTAGTGCATCCCAAGATGGAAGATTAATAGTGTGGAATGCTCTAACAAGCCAGAAAACTCATGCTATAAAGCTTCCTTGTGCATGGGTCATGACGTGTGCTTTCTCACCAACTGGTCAATCTGTTGCTTGTGGGGGCCTTGACAGTGTTTGCTCTATTTTCAATCTTAATTCTCCCACTGATAGGGATGGGAATCTAAATGTTTCACGGATGCTTAGTGGACATAAAGGTTATGTTTCATCTTGTCAGTATGTTCCAGGTGAAGACACTCACTTAATCACTGGTTCTGGAGATCAGACATGTGTTTTATGGGATATTACTACTGGCCTTAGAACATCTGTTTTTGGAGGCGAGTTTCAGTCTGGACATACTGCAGATGTACTTAGCATTTCCATTAATGGATCCAACTCCAAATTGTTTGTATCTGGTTCTTGCGATGCGACTGCCAGATTGTGGGACACTCGTGTGGCAAGTCGAGCAGTGCGGACATTTCACGGCCACGAGGGAGATGTTAATTCTGTCAAATTCTTTCCTGATGGAAATAGATTTGGAACTGGCTCAGAGGATGGAACTTGCAGATTATTTGACATTAGGACCGGACACCAACTTCAAGTATATAATCAGCAACACCAAGACAACGAAATGGCACATGTGACGTCCATTGCATTTTCCATATCCGGAAGACTTCTTATTGCTGGCTATACAAATGGTGATTGCTATGTTTGGGATACTTTATTGGCTAAGGTGGTCTTGAATCTAGGATCTCTTCAAAACTCTCATGAGGGCAGGATCACCTGTTTGGGTATGTCTGCTGATGGAAGCGCTTTATGTACAGGAAGTTGGGACACAAATTTAAAGATATGGGCATTTGGAGGGCATAGGAAGGTGATTTGACTCCATTGTTAGGGCTTCACCTTGTAATGATGCTTGTGATATTGACTTTGATCCAGAATTGGAAGGCAAAGTTTATCTCCATGTTTATAACCTTTAGCAGTGGAACTAGTGCAGCCTTTATTTATCTCCATGCTCATTGGTTCGTGTGTGTGATTTAGGTATATATATACCTTTAAACCAAAACAGAGGACTATTTAATTTTCTGTCTCCTCAATTTAACTATTTGAAGTATGTGTTTGGTTCACATTGGAAGAACTAAATGTACTAGTATGTTTATAGTGGTTGAATCAGATTTGGATCAGGTAAGGGGGTGTTTGGATCCCCATTGTAAAATCGAATCTTCTTCCTGTTGAAGCAACTCTTGTGTAACTTTTGGAAACCAAAAAAAAAANCTACATTAAAAACCTTTCATAACC
TARGET=1510,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1254
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.124655
PRIMER_LEFT_SEQUENCE=TTTGACTCCATTGTTAGGGCTT
PRIMER_RIGHT_SEQUENCE=TCTTCCAATGTGAACCAAACAC
PRIMER_LEFT=1345,22
PRIMER_RIGHT=1603,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.875
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=0.2860
PRIMER_LEFT_1_PENALTY=0.161322
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=TAAAGATATGGGCATTTGGAGG
PRIMER_RIGHT_1_SEQUENCE=TCTTCCAATGTGAACCAAACAC
PRIMER_LEFT_1=1309,22
PRIMER_RIGHT_1=1603,22
PRIMER_LEFT_1_TM=60.161
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=0.4972
PRIMER_LEFT_2_PENALTY=0.372557
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=AGGGCTTCACCTTGTAATGATG
PRIMER_RIGHT_2_SEQUENCE=TCTTCCAATGTGAACCAAACAC
PRIMER_LEFT_2=1360,22
PRIMER_RIGHT_2=1603,22
PRIMER_LEFT_2_TM=60.373
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.6074
PRIMER_LEFT_3_PENALTY=0.482784
PRIMER_RIGHT_3_PENALTY=0.124655
PRIMER_LEFT_3_SEQUENCE=TTGACTCCATTGTTAGGGCTTC
PRIMER_RIGHT_3_SEQUENCE=TCTTCCAATGTGAACCAAACAC
PRIMER_LEFT_3=1346,22
PRIMER_RIGHT_3=1603,22
PRIMER_LEFT_3_TM=60.483
PRIMER_RIGHT_3_TM=59.875
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=0.6356
PRIMER_LEFT_4_PENALTY=0.510985
PRIMER_RIGHT_4_PENALTY=0.124655
PRIMER_LEFT_4_SEQUENCE=GGCTTCACCTTGTAATGATGCT
PRIMER_RIGHT_4_SEQUENCE=TCTTCCAATGTGAACCAAACAC
PRIMER_LEFT_4=1362,22
PRIMER_RIGHT_4=1603,22
PRIMER_LEFT_4_TM=60.511
PRIMER_RIGHT_4_TM=59.875
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_05814 Ps_contig_mira-and-tgicl-ass_franssen_40028
SEQUENCE=ACCTTTTACTAAAATAGAAACTTTCTATAAAGGCAACGAACGAAACACNTTTTTTTTTATAAACAGAGCAATCCTTTAATAAGGCAGTATGATATTTAGAAGCAGTAGTTCATAACTTGATATTCATAAAAACAAACAATAAACAAGGAAAAGTAAAAGAAACTACAAGAACAATAATCATCATGTACTCAAAGTCAAAATACATTTGTAATCAACCCAATTTGTGCAAAGTAATATCTCCCCTCAAAAGCACGCATCCAAAAGACAGCAGCAGCAGAGAGCAGCCAAGCAGCCTTCCAAGCAACCAGCACCGGATGAATTTTGCTGTTGTTGAGGCGGAGGCTGAGCATACTGAGGAGCATATTGCGGTGGATAGCCTTGCTGTGGATAGCCTTGCGGTGGATAGCCCTGCTGTGGAGGGTAGCCTTGCGGCGGATACCCCGGCGGAGGGTAAGCGTCTTTTCCATAAGACTCTTGCTGCGGATAACCTTGCGGCGGAGGAACGCCGACGGGCGGTTGTTGTTGGTTGTAGTAACTCATTTGGGTGGCGGAAAGAGAAGAAGAAAGGGTTGGAAGTTGGAGGATAGAAATAGGTGATTTGGATTTGTTTTTGAGAAAATATAGATGGAGAATTTATTTATATCATCGACTAAGTAAATACCCCGA
TARGET=266,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2959
PRIMER_LEFT_PENALTY=1.282148
PRIMER_RIGHT_PENALTY=0.013714
PRIMER_LEFT_SEQUENCE=TGCAAAGTAATATCTCCCCTCA
PRIMER_RIGHT_SEQUENCE=ACCGCAATATGCTCCTCAGTAT
PRIMER_LEFT=224,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=58.718
PRIMER_RIGHT_TM=60.014
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=5.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=146
PRIMER_PAIR_PENALTY_1=1.4708
PRIMER_LEFT_1_PENALTY=1.457080
PRIMER_RIGHT_1_PENALTY=0.013714
PRIMER_LEFT_1_SEQUENCE=ATATCTCCCCTCAAAAGCACG
PRIMER_RIGHT_1_SEQUENCE=ACCGCAATATGCTCCTCAGTAT
PRIMER_LEFT_1=233,21
PRIMER_RIGHT_1=369,22
PRIMER_LEFT_1_TM=60.457
PRIMER_RIGHT_1_TM=60.014
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=5.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=1.4991
PRIMER_LEFT_2_PENALTY=1.485357
PRIMER_RIGHT_2_PENALTY=0.013714
PRIMER_LEFT_2_SEQUENCE=TGTGCAAAGTAATATCTCCCCTC
PRIMER_RIGHT_2_SEQUENCE=ACCGCAATATGCTCCTCAGTAT
PRIMER_LEFT_2=222,23
PRIMER_RIGHT_2=369,22
PRIMER_LEFT_2_TM=59.515
PRIMER_RIGHT_2_TM=60.014
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=5.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=1.4991
PRIMER_LEFT_3_PENALTY=1.485357
PRIMER_RIGHT_3_PENALTY=0.013714
PRIMER_LEFT_3_SEQUENCE=GTGCAAAGTAATATCTCCCCTCA
PRIMER_RIGHT_3_SEQUENCE=ACCGCAATATGCTCCTCAGTAT
PRIMER_LEFT_3=223,23
PRIMER_RIGHT_3=369,22
PRIMER_LEFT_3_TM=59.515
PRIMER_RIGHT_3_TM=60.014
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.3000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=147
PRIMER_PAIR_PENALTY_4=1.6494
PRIMER_LEFT_4_PENALTY=1.635664
PRIMER_RIGHT_4_PENALTY=0.013714
PRIMER_LEFT_4_SEQUENCE=AATATCTCCCCTCAAAAGCACG
PRIMER_RIGHT_4_SEQUENCE=ACCGCAATATGCTCCTCAGTAT
PRIMER_LEFT_4=232,22
PRIMER_RIGHT_4=369,22
PRIMER_LEFT_4_TM=61.636
PRIMER_RIGHT_4_TM=60.014
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=5.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=138
=
PRIMER_SEQUENCE_ID=SSR_05815 Ps_contig_mira-and-tgicl-ass_franssen_40039
SEQUENCE=TGATATTAAAGATGCAATTAGGAAGTTTAGTTTTCCTTATTTGCGAAGATGTGCATTGTTGTGGAAGATACTCTATTCTTCTATCCCAGCACCATTCTGTGATGAGGGAAATATGTCAAATAGACCATTGATTATCCCAAGGGACACAATGGGCAGTGCTGATATTAACATGTTTGAGATTATTAAAATTCAAGAGCTTGAGAATATGTTTAAATTCCCCTCTGGATGTGGTTCTCAAAGATGAACTTTCAAGATCTTCAGTTTCTATTTGGTGTCGTCATTTTTGCAAGGAATTTGAATCACAGAGAATTCAACGTAATATTCATGTAACTCCTGCAGTTCCATTTAGGTTAATGAAATTACCAAATGTTTATCAGGATCTCTTGCAGAGGTGTATCAAACAGCGCTGCCCTGAGTGCAAATCACATCTTGATGATCCTGCAGTGTGTCTATTGTGCGGTAGATTGTGCTCCCCAAGCTGGAAATCATGCTGCAGGAAAAGTGGATGCCAAACTCATTCTGTAACTTGTGGAGCTGGTACCGGAGTGTTTCTGTTAATTAAGAGAACTACAATCCTACTACAGAGATCTGCACGTCAGGCCCCTTGGCCTTCTCCTTACTTGGATACTTTTGGCGAGGAGGATTTTGAAATGAACAGAGGCAAGCCAATCTTCCTAAATGAGGAGCGTTATGCTGCTTTGACATATATGGTTGCTTCTCATGGTCTGGACCGGAGTTCTAAGGTTCTTGGCCAGACAACTATTGGTTCCTTCTTCCTGGTTTAGTTCAAGTTATACTTGTTCAGTTGCAGTATTGAGATTTTTTGGCTGGACAAATCTCTGCAGGGAAGAAATAGGTTCTCTAAATGCAAATTTATCATGTTATAAACTTCTCCCTGGATAATCAAAAGAGCTTTAGTCCATGCTATGTCTAAGTGGAAATTGTACAGCCCTTTCAGAATGATATACTTGGTGCATAAAGCTTCAGRATGTTAGCTACCTGCTGGCCGTTGCTGTACAGAATTTCCATGATAAAAGAATTGCAAGCTTAATTGTGCTTCATGCTTATGGTTTAGCTGTCTGAGAAGACTATGTTGACTCCCTCCTCTCTAACTCGGATGTTTGGCTGGCTCGTCCTCTCTTCCTGGGTTACAAAACCAAAATAATTTCTTATGTTTGGGCATATTGCATAGAAGTTGATATATAAGTGTTGGTTTGATTGTAGAATATTTGAGAAATGATGTTACTAAAATTAACAGTTAAGNTTTGGGCTGTTTTCAAGCTCATTGAGCCATGACCTCTGTTACTCTATGTCCTTCAAAATAAATAAATTTAAGT
TARGET=1320,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05816 Ps_contig_mira-and-tgicl-ass_franssen_40048
SEQUENCE=GATTTTCATCTCTTCTARTGATAGGTCGTTCCCTAATTCACCTTCACTTGAATTATCATCAGTGTCAGCTGGATCATCACTAGAACCTGGCTTCAATTCCTTCATCTCGCGTAGAACGTCTTTCACGGAAACCGCAACCTTCGTCATTCCTCGGCCAATTGCTGTAATGTTTGACGAAGGAGTCTTTTTTAGGGCAGAGCACGCTTCCCATACAACACCCACCAGTTGTGGAACTGATTGTTTCTGGACTTCATTATGGGATCCATATAAAGAGACAGTTTCCTTCATCAACCTGAAGCAGGAATCAACAACTTGCTTGACAGATTCATGAATACTGGAAGACAAGGTAGGTCCCGCACCTACCATGCTGCCATGAGAAACCAAGAGGAAACCTTGTAGATTGTTGAAGTAGGATGTCATATTTTCCTCAATTGCTTTTGACTCTGGTTTGTCTCCAGACCAAAGCATTCCAACTGTTGTAGCTTGTTTGGAAACTTGATCGGCCATTTTAAGGACGTCTTCCCAGGAGACTTTGGAATCGAAGGAAGAAGAAGAAGGAGTTTGATCCAAAACTTGTAGGGTTTCATGGATGGTGGTGAGGTGGGAACCTAGGAGAAGGTTGAGCCGCTCTTTCTCTGCTCTTGCCGGCATCTTGCTTCTGCTTGATAGCTCTCTTCTACCTCGACGGGGACGGCGGCGTTGTTAGTGTGAACCTGTGCTTATTGTGATTTGTGAACGATACNAATCATA
TARGET=545,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05817 Ps_contig_mira-and-tgicl-ass_franssen_40052
SEQUENCE=TCTCTCCACCTCTCTCTCTCTTACATCACACATACACTTTCTCTCTNATACTATATTTTCTCATTGAAACTTCCTCTGTTTCGGATCACCACATGACCGTTTCCTTCTTCTTTTTCTCTCTCTCTGCAACAAACACTTTTCTCATTTTTCTATTCTTTCATAGTTGTGGGATTCTTTGACCCACTTCTTTCTTTTCCCAAAAACACTATTTTTCTTTCTTTTACAACCCCAAGGTACCCTTTTTCTTTTCCCATTCTTGTTCTTTCGAAGATTCTACCATCTGGGTCGTTTTCGTTGATGTCTGTAACGACCATTGAAGGATTGGTTTCGAATTCCGAAAATAAGATGGGGTTGGATGACAATGTCAAAAGCAATGAAGACGATAATAATAATATTAATAACACTTCTCGTGTGGAAGGAAGTGATAACGAAGAAGGAGGTACTCTTAGCAGATACATGAGTGAGAGTTCTGTTGCTGCTACTGAGGAGGAGGAGGAGGATGAAGATAGGAAGATTGAATTGGGTCCACAATGTACCTTAAAGGAACAACTTGAAAAAGATAAGGATGATGAGAGCTTGAGGAGGTGGAAGGAGCAGCTTCTTGGAGGTGTTGATATCAATGCTGTTGGAGAAACCCTGGAGCCGGATGTGAAGATCGTGAGCCTTGCAATAAAATCTGCTGGTAGACCTGATATTGTTCTTCCGATACCAGAAGATGGAAATCCAAAGGGTTTATGGTTTACTTTGAAAGAAGGTAGCCGTTACAGGTTGGTTTTCACTTTTCAGGTCAACCATAACATTGTTTCTGGTCTCAAATACACCAACACTGTCTGGAAAACTGGTATCAAAGTTGATAGCAGTAAAGAAATGATTGGAACCTTCAGCCCTCAATCTGAGACTTACACTCATGAGATGCCTGAAGAGACGACACCGTCTGGAATTTTCGCTAGAGGGGCATATACAGCTAGAAGTAAGTTTGTTGATGATGATAACAAGAGCTACTTGGAGATCAACTACACTTTTGATATCAGAAAGGATTGGCAGTAACTAGGATTCAGATATATAGCTGAATTCTTCTCCTCCTACATTGATTCATATGTTGAATTAATCCTTAATCATTGTTATTTAATGCTGAAATCTCCTTCAGTGTTTTAGTTTCTTCTTTTTCATTGTTTATTTTGTTTATTTCTAATCCTGTCTGAGACTGGTAATCTTAACAATGATGAGAGGATTTTTGAAGGATGATATTGGTATTAGTATTCATTTC
TARGET=10,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05818 Ps_contig_mira-and-tgicl-ass_franssen_40052
SEQUENCE=TCTCTCCACCTCTCTCTCTCTTACATCACACATACACTTTCTCTCTNATACTATATTTTCTCATTGAAACTTCCTCTGTTTCGGATCACCACATGACCGTTTCCTTCTTCTTTTTCTCTCTCTCTGCAACAAACACTTTTCTCATTTTTCTATTCTTTCATAGTTGTGGGATTCTTTGACCCACTTCTTTCTTTTCCCAAAAACACTATTTTTCTTTCTTTTACAACCCCAAGGTACCCTTTTTCTTTTCCCATTCTTGTTCTTTCGAAGATTCTACCATCTGGGTCGTTTTCGTTGATGTCTGTAACGACCATTGAAGGATTGGTTTCGAATTCCGAAAATAAGATGGGGTTGGATGACAATGTCAAAAGCAATGAAGACGATAATAATAATATTAATAACACTTCTCGTGTGGAAGGAAGTGATAACGAAGAAGGAGGTACTCTTAGCAGATACATGAGTGAGAGTTCTGTTGCTGCTACTGAGGAGGAGGAGGAGGATGAAGATAGGAAGATTGAATTGGGTCCACAATGTACCTTAAAGGAACAACTTGAAAAAGATAAGGATGATGAGAGCTTGAGGAGGTGGAAGGAGCAGCTTCTTGGAGGTGTTGATATCAATGCTGTTGGAGAAACCCTGGAGCCGGATGTGAAGATCGTGAGCCTTGCAATAAAATCTGCTGGTAGACCTGATATTGTTCTTCCGATACCAGAAGATGGAAATCCAAAGGGTTTATGGTTTACTTTGAAAGAAGGTAGCCGTTACAGGTTGGTTTTCACTTTTCAGGTCAACCATAACATTGTTTCTGGTCTCAAATACACCAACACTGTCTGGAAAACTGGTATCAAAGTTGATAGCAGTAAAGAAATGATTGGAACCTTCAGCCCTCAATCTGAGACTTACACTCATGAGATGCCTGAAGAGACGACACCGTCTGGAATTTTCGCTAGAGGGGCATATACAGCTAGAAGTAAGTTTGTTGATGATGATAACAAGAGCTACTTGGAGATCAACTACACTTTTGATATCAGAAAGGATTGGCAGTAACTAGGATTCAGATATATAGCTGAATTCTTCTCCTCCTACATTGATTCATATGTTGAATTAATCCTTAATCATTGTTATTTAATGCTGAAATCTCCTTCAGTGTTTTAGTTTCTTCTTTTTCATTGTTTATTTTGTTTATTTCTAATCCTGTCTGAGACTGGTAATCTTAACAATGATGAGAGGATTTTTGAAGGATGATATTGGTATTAGTATTCATTTC
TARGET=115,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1398
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=TGAAACTTCCTCTGTTTCGGAT
PRIMER_RIGHT_SEQUENCE=CCTTCAATGGTCGTTACAGACA
PRIMER_LEFT=64,22
PRIMER_RIGHT=319,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.8000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=256
PRIMER_PAIR_PENALTY_1=0.1468
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=TGAAACTTCCTCTGTTTCGGAT
PRIMER_RIGHT_1_SEQUENCE=GGTCGTTACAGACATCAACGAA
PRIMER_LEFT_1=64,22
PRIMER_RIGHT_1=311,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.8000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.1892
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.079714
PRIMER_LEFT_2_SEQUENCE=TGAAACTTCCTCTGTTTCGGAT
PRIMER_RIGHT_2_SEQUENCE=ATGGTCGTTACAGACATCAACG
PRIMER_LEFT_2=64,22
PRIMER_RIGHT_2=313,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.920
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.8000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.2486
PRIMER_LEFT_3_PENALTY=0.109510
PRIMER_RIGHT_3_PENALTY=0.139129
PRIMER_LEFT_3_SEQUENCE=TGAAACTTCCTCTGTTTCGGAT
PRIMER_RIGHT_3_SEQUENCE=CAATCCTTCAATGGTCGTTACA
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=323,22
PRIMER_LEFT_3_TM=60.110
PRIMER_RIGHT_3_TM=59.861
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.8000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.3481
PRIMER_LEFT_4_PENALTY=0.109510
PRIMER_RIGHT_4_PENALTY=0.238588
PRIMER_LEFT_4_SEQUENCE=TGAAACTTCCTCTGTTTCGGAT
PRIMER_RIGHT_4_SEQUENCE=AAAAGAAAAAGGGTACCTTGGG
PRIMER_LEFT_4=64,22
PRIMER_RIGHT_4=248,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.761
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.8000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_05819 Ps_contig_mira-and-tgicl-ass_franssen_40052
SEQUENCE=TCTCTCCACCTCTCTCTCTCTTACATCACACATACACTTTCTCTCTNATACTATATTTTCTCATTGAAACTTCCTCTGTTTCGGATCACCACATGACCGTTTCCTTCTTCTTTTTCTCTCTCTCTGCAACAAACACTTTTCTCATTTTTCTATTCTTTCATAGTTGTGGGATTCTTTGACCCACTTCTTTCTTTTCCCAAAAACACTATTTTTCTTTCTTTTACAACCCCAAGGTACCCTTTTTCTTTTCCCATTCTTGTTCTTTCGAAGATTCTACCATCTGGGTCGTTTTCGTTGATGTCTGTAACGACCATTGAAGGATTGGTTTCGAATTCCGAAAATAAGATGGGGTTGGATGACAATGTCAAAAGCAATGAAGACGATAATAATAATATTAATAACACTTCTCGTGTGGAAGGAAGTGATAACGAAGAAGGAGGTACTCTTAGCAGATACATGAGTGAGAGTTCTGTTGCTGCTACTGAGGAGGAGGAGGAGGATGAAGATAGGAAGATTGAATTGGGTCCACAATGTACCTTAAAGGAACAACTTGAAAAAGATAAGGATGATGAGAGCTTGAGGAGGTGGAAGGAGCAGCTTCTTGGAGGTGTTGATATCAATGCTGTTGGAGAAACCCTGGAGCCGGATGTGAAGATCGTGAGCCTTGCAATAAAATCTGCTGGTAGACCTGATATTGTTCTTCCGATACCAGAAGATGGAAATCCAAAGGGTTTATGGTTTACTTTGAAAGAAGGTAGCCGTTACAGGTTGGTTTTCACTTTTCAGGTCAACCATAACATTGTTTCTGGTCTCAAATACACCAACACTGTCTGGAAAACTGGTATCAAAGTTGATAGCAGTAAAGAAATGATTGGAACCTTCAGCCCTCAATCTGAGACTTACACTCATGAGATGCCTGAAGAGACGACACCGTCTGGAATTTTCGCTAGAGGGGCATATACAGCTAGAAGTAAGTTTGTTGATGATGATAACAAGAGCTACTTGGAGATCAACTACACTTTTGATATCAGAAAGGATTGGCAGTAACTAGGATTCAGATATATAGCTGAATTCTTCTCCTCCTACATTGATTCATATGTTGAATTAATCCTTAATCATTGTTATTTAATGCTGAAATCTCCTTCAGTGTTTTAGTTTCTTCTTTTTCATTGTTTATTTTGTTTATTTCTAATCCTGTCTGAGACTGGTAATCTTAACAATGATGAGAGGATTTTTGAAGGATGATATTGGTATTAGTATTCATTTC
TARGET=383,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0472
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.016950
PRIMER_LEFT_SEQUENCE=TGTCTGTAACGACCATTGAAGG
PRIMER_RIGHT_SEQUENCE=AGGTACATTGTGGACCCAATTC
PRIMER_LEFT=298,22
PRIMER_RIGHT=537,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=59.983
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.0510
PRIMER_LEFT_1_PENALTY=0.030276
PRIMER_RIGHT_1_PENALTY=0.020747
PRIMER_LEFT_1_SEQUENCE=TGTCTGTAACGACCATTGAAGG
PRIMER_RIGHT_1_SEQUENCE=CTCCTCAAGCTCTCATCATCCT
PRIMER_LEFT_1=298,22
PRIMER_RIGHT_1=583,22
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=59.979
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.0543
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.016950
PRIMER_LEFT_2_SEQUENCE=TTCGTTGATGTCTGTAACGACC
PRIMER_RIGHT_2_SEQUENCE=AGGTACATTGTGGACCCAATTC
PRIMER_LEFT_2=290,22
PRIMER_RIGHT_2=537,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=59.983
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=0.0553
PRIMER_LEFT_3_PENALTY=0.038361
PRIMER_RIGHT_3_PENALTY=0.016950
PRIMER_LEFT_3_SEQUENCE=TCGAAGATTCTACCATCTGGGT
PRIMER_RIGHT_3_SEQUENCE=AGGTACATTGTGGACCCAATTC
PRIMER_LEFT_3=264,22
PRIMER_RIGHT_3=537,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=59.983
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.0553
PRIMER_LEFT_4_PENALTY=0.038361
PRIMER_RIGHT_4_PENALTY=0.016950
PRIMER_LEFT_4_SEQUENCE=GAAGATTCTACCATCTGGGTCG
PRIMER_RIGHT_4_SEQUENCE=AGGTACATTGTGGACCCAATTC
PRIMER_LEFT_4=266,22
PRIMER_RIGHT_4=537,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=59.983
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_05820 Ps_contig_mira-and-tgicl-ass_franssen_40052
SEQUENCE=TCTCTCCACCTCTCTCTCTCTTACATCACACATACACTTTCTCTCTNATACTATATTTTCTCATTGAAACTTCCTCTGTTTCGGATCACCACATGACCGTTTCCTTCTTCTTTTTCTCTCTCTCTGCAACAAACACTTTTCTCATTTTTCTATTCTTTCATAGTTGTGGGATTCTTTGACCCACTTCTTTCTTTTCCCAAAAACACTATTTTTCTTTCTTTTACAACCCCAAGGTACCCTTTTTCTTTTCCCATTCTTGTTCTTTCGAAGATTCTACCATCTGGGTCGTTTTCGTTGATGTCTGTAACGACCATTGAAGGATTGGTTTCGAATTCCGAAAATAAGATGGGGTTGGATGACAATGTCAAAAGCAATGAAGACGATAATAATAATATTAATAACACTTCTCGTGTGGAAGGAAGTGATAACGAAGAAGGAGGTACTCTTAGCAGATACATGAGTGAGAGTTCTGTTGCTGCTACTGAGGAGGAGGAGGAGGATGAAGATAGGAAGATTGAATTGGGTCCACAATGTACCTTAAAGGAACAACTTGAAAAAGATAAGGATGATGAGAGCTTGAGGAGGTGGAAGGAGCAGCTTCTTGGAGGTGTTGATATCAATGCTGTTGGAGAAACCCTGGAGCCGGATGTGAAGATCGTGAGCCTTGCAATAAAATCTGCTGGTAGACCTGATATTGTTCTTCCGATACCAGAAGATGGAAATCCAAAGGGTTTATGGTTTACTTTGAAAGAAGGTAGCCGTTACAGGTTGGTTTTCACTTTTCAGGTCAACCATAACATTGTTTCTGGTCTCAAATACACCAACACTGTCTGGAAAACTGGTATCAAAGTTGATAGCAGTAAAGAAATGATTGGAACCTTCAGCCCTCAATCTGAGACTTACACTCATGAGATGCCTGAAGAGACGACACCGTCTGGAATTTTCGCTAGAGGGGCATATACAGCTAGAAGTAAGTTTGTTGATGATGATAACAAGAGCTACTTGGAGATCAACTACACTTTTGATATCAGAAAGGATTGGCAGTAACTAGGATTCAGATATATAGCTGAATTCTTCTCCTCCTACATTGATTCATATGTTGAATTAATCCTTAATCATTGTTATTTAATGCTGAAATCTCCTTCAGTGTTTTAGTTTCTTCTTTTTCATTGTTTATTTTGTTTATTTCTAATCCTGTCTGAGACTGGTAATCTTAACAATGATGAGAGGATTTTTGAAGGATGATATTGGTATTAGTATTCATTTC
TARGET=484,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0472
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.016950
PRIMER_LEFT_SEQUENCE=TGTCTGTAACGACCATTGAAGG
PRIMER_RIGHT_SEQUENCE=AGGTACATTGTGGACCCAATTC
PRIMER_LEFT=298,22
PRIMER_RIGHT=537,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=59.983
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=240
PRIMER_PAIR_PENALTY_1=0.0510
PRIMER_LEFT_1_PENALTY=0.030276
PRIMER_RIGHT_1_PENALTY=0.020747
PRIMER_LEFT_1_SEQUENCE=TGTCTGTAACGACCATTGAAGG
PRIMER_RIGHT_1_SEQUENCE=CTCCTCAAGCTCTCATCATCCT
PRIMER_LEFT_1=298,22
PRIMER_RIGHT_1=583,22
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=59.979
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.0543
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.016950
PRIMER_LEFT_2_SEQUENCE=TTCGTTGATGTCTGTAACGACC
PRIMER_RIGHT_2_SEQUENCE=AGGTACATTGTGGACCCAATTC
PRIMER_LEFT_2=290,22
PRIMER_RIGHT_2=537,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=59.983
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=0.0553
PRIMER_LEFT_3_PENALTY=0.038361
PRIMER_RIGHT_3_PENALTY=0.016950
PRIMER_LEFT_3_SEQUENCE=TCGAAGATTCTACCATCTGGGT
PRIMER_RIGHT_3_SEQUENCE=AGGTACATTGTGGACCCAATTC
PRIMER_LEFT_3=264,22
PRIMER_RIGHT_3=537,22
PRIMER_LEFT_3_TM=59.962
PRIMER_RIGHT_3_TM=59.983
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=0.0553
PRIMER_LEFT_4_PENALTY=0.038361
PRIMER_RIGHT_4_PENALTY=0.016950
PRIMER_LEFT_4_SEQUENCE=GAAGATTCTACCATCTGGGTCG
PRIMER_RIGHT_4_SEQUENCE=AGGTACATTGTGGACCCAATTC
PRIMER_LEFT_4=266,22
PRIMER_RIGHT_4=537,22
PRIMER_LEFT_4_TM=59.962
PRIMER_RIGHT_4_TM=59.983
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_05821 Ps_contig_mira-and-tgicl-ass_franssen_40053
SEQUENCE=TCCATGACCAGGTCNGAAACATGCACAATTTCGTTTATCTAACTGAATATTCTCNGTTTACTGATAACAATGTGCGGCGGCGGTGGTGGTGGTTGTGGCGGTGCAGGTGTTCGACCTNCTTTTAACGCAGAGTACGGCGGGGACGGCGATGGCGAGGACGCTTAAGGATATGGACACGTGTACGGTGAGCAATTCGTTCTGCGTGCAATCGGATATAGCAATATCGTTAGGATACGCGGCGTTTCTGTTTCTAGGGTTTACTTCGCTGTTATCTGGATTCCGTCTCGTTTGTTTCATCATCAACGGTTCTCGTTTTCATCTCTGAATGAATTTTTTCCGAGGAGTTTTGCGTGGAAACGACGCCGTAGATGAATGAATGCCGGGAATATTTGTGTGGGGTCAGGAGCATTCTTTTCTTGGTACTCTCAATTGGATGTGATTTGATTTGGACCGTCATGATTGCAGAAGAACTTGAGTTGAAAATATGCGCGTGACTTTGATTTTTGAGCTTATACCATAGATTATTGTAGCAAAAGTATTTGTAACACCTATTTTATNGCAATAATTCTATTGATTTTANCCACTC
TARGET=82,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0403
PRIMER_LEFT_PENALTY=0.026872
PRIMER_RIGHT_PENALTY=0.013401
PRIMER_LEFT_SEQUENCE=TTTACTGATAACAATGTGCGGC
PRIMER_RIGHT_SEQUENCE=TGTCCATATCCTTAAGCGTCCT
PRIMER_LEFT=56,22
PRIMER_RIGHT=175,22
PRIMER_LEFT_TM=60.027
PRIMER_RIGHT_TM=59.987
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=12.9000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=0.0492
PRIMER_LEFT_1_PENALTY=0.026872
PRIMER_RIGHT_1_PENALTY=0.022341
PRIMER_LEFT_1_SEQUENCE=TTTACTGATAACAATGTGCGGC
PRIMER_RIGHT_1_SEQUENCE=GAGAACCGTTGATGATGAAACA
PRIMER_LEFT_1=56,22
PRIMER_RIGHT_1=310,22
PRIMER_LEFT_1_TM=60.027
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=12.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=255
PRIMER_PAIR_PENALTY_2=0.0523
PRIMER_LEFT_2_PENALTY=0.026872
PRIMER_RIGHT_2_PENALTY=0.025403
PRIMER_LEFT_2_SEQUENCE=TTTACTGATAACAATGTGCGGC
PRIMER_RIGHT_2_SEQUENCE=TATTGCTATATCCGATTGCACG
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=222,22
PRIMER_LEFT_2_TM=60.027
PRIMER_RIGHT_2_TM=59.975
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=12.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.0610
PRIMER_LEFT_3_PENALTY=0.026872
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=TTTACTGATAACAATGTGCGGC
PRIMER_RIGHT_3_SEQUENCE=AAACGAGACGGAATCCAGATAA
PRIMER_LEFT_3=56,22
PRIMER_RIGHT_3=289,22
PRIMER_LEFT_3_TM=60.027
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=12.9000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.1295
PRIMER_LEFT_4_PENALTY=0.026872
PRIMER_RIGHT_4_PENALTY=0.102638
PRIMER_LEFT_4_SEQUENCE=TTTACTGATAACAATGTGCGGC
PRIMER_RIGHT_4_SEQUENCE=GAGACGGAATCCAGATAACAGC
PRIMER_LEFT_4=56,22
PRIMER_RIGHT_4=285,22
PRIMER_LEFT_4_TM=60.027
PRIMER_RIGHT_4_TM=60.103
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=12.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_05822 Ps_contig_mira-and-tgicl-ass_franssen_40067
SEQUENCE=AGGAGAGGTTATGAACTAAAAATACACCTCTCCCATATGGGTATCCTCACAGAATCTGCAACTGGTGCAAGAACTTGGCAAAAGGGGGGCCTAAATGACACAAGAGGCTGCAATTAGTTATTGTTGTTGTTGTACTTCATCGGGTGATCTCTACTATGACTATTAATTTGTYATGTTTCTTTGTATATATTACGATTTTATTTTTTTGGGTTTCTTCATAAGTTTCTTGTAGACGTCATATGGAATATTCCTAGATGATTGTCGTGAACATTTTGACTYGATGATTGGTTGGATTGTG
TARGET=121,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05823 Ps_contig_mira-and-tgicl-ass_franssen_40068
SEQUENCE=ATGTTTATATATATGTATATCACTTTGTTTGACCCTTNGTAAACTTGTGGTGTTGAAGTGAGTGGCTGCTATAAATGTTCTAGAGAAGTACTTGTTTAACATTCCACTGCATCTTCTTTGTTATACATCCTCATCTTATAGTTTGAGAGAGAGAGAGAAAGAAAGAGTTGATGGGAAATTGCCAAGCTATTGATACAGCAACTCTTGTAATACAACAACCAAATGGAAAAGAAGAAAGATTATATTGGCCAGTAAGTGCTAGTGAAGTCATGAAAACAAACCCTAATCACTATGTTGCTCTTCTTATCTCCACAACATTATGCACTTCAAAAGATAGAGAAAATTGTTCAAATAAGACTCACAACAACAACAACAACAACAACCCTGTTCGTATTACACGTATCAAGCTTCTTAAACCAACAGATACACTTATGCTTGGTCAAGTTTATAGACTCATCTCAACTCAAGAAGTTACAAAGGGTATGTGGGCAAAGAAACAAGCAAAAATGAAGAGAAGCTCATCAGAATCATCTCAAAAATCAAATCAGGTTAAGGAAAGAATTGACAAAGAAGCTGAAAGGTCTCAACCACCCGAAAACAATAAGGAAAGGAAATCTGAAAGACATGAGTCAAGGACAAAAGGATCAAGTAATGGTGGAAATCATAACAAGTCAAGAACATGGCAACCCTCTTTACAAAGCATCACTGAATCTTCCAGCTGATATCTGTTTTTCTTTTTTCAATCTTACTCACATGTTGAGAAACTATGATTGCTTAGATCTGTGGAAATTTAATTAAATATACCACATTTTAGGGAATCTAGAGAGAAATCATAAAAATCTGTCATGCATAATAATTGTGTATAGTAAAAGTTGACATATAATATTCGATATCAGTTAATGTATTCAGATATTAATCTTCACTCAAATCAAACTTATTATTATCTGTATTATATTCTNATTTCTGTATTTTTTTAATGTATGGTTTCAGACGAATAATATGTCGACATCTTGACT
TARGET=145,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1060
PRIMER_LEFT_PENALTY=0.057411
PRIMER_RIGHT_PENALTY=0.048570
PRIMER_LEFT_SEQUENCE=TGTTGAAGTGAGTGGCTGCTAT
PRIMER_RIGHT_SEQUENCE=GCAATTTCCCATCAACTCTTTC
PRIMER_LEFT=50,22
PRIMER_RIGHT=181,22
PRIMER_LEFT_TM=59.943
PRIMER_RIGHT_TM=59.951
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.9223
PRIMER_LEFT_1_PENALTY=0.757660
PRIMER_RIGHT_1_PENALTY=0.164595
PRIMER_LEFT_1_SEQUENCE=CATTCCACTGCATCTTCTTTGT
PRIMER_RIGHT_1_SEQUENCE=AGGGTTGTTGTTGTTGTTGTTG
PRIMER_LEFT_1=99,22
PRIMER_RIGHT_1=385,22
PRIMER_LEFT_1_TM=59.242
PRIMER_RIGHT_1_TM=59.835
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.9223
PRIMER_LEFT_2_PENALTY=0.757660
PRIMER_RIGHT_2_PENALTY=0.164595
PRIMER_LEFT_2_SEQUENCE=ACATTCCACTGCATCTTCTTTG
PRIMER_RIGHT_2_SEQUENCE=AGGGTTGTTGTTGTTGTTGTTG
PRIMER_LEFT_2=98,22
PRIMER_RIGHT_2=385,22
PRIMER_LEFT_2_TM=59.242
PRIMER_RIGHT_2_TM=59.835
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.9223
PRIMER_LEFT_3_PENALTY=0.757660
PRIMER_RIGHT_3_PENALTY=0.164595
PRIMER_LEFT_3_SEQUENCE=CATTCCACTGCATCTTCTTTGT
PRIMER_RIGHT_3_SEQUENCE=CAGGGTTGTTGTTGTTGTTGTT
PRIMER_LEFT_3=99,22
PRIMER_RIGHT_3=386,22
PRIMER_LEFT_3_TM=59.242
PRIMER_RIGHT_3_TM=59.835
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=288
PRIMER_PAIR_PENALTY_4=0.9223
PRIMER_LEFT_4_PENALTY=0.757660
PRIMER_RIGHT_4_PENALTY=0.164595
PRIMER_LEFT_4_SEQUENCE=ACATTCCACTGCATCTTCTTTG
PRIMER_RIGHT_4_SEQUENCE=CAGGGTTGTTGTTGTTGTTGTT
PRIMER_LEFT_4=98,22
PRIMER_RIGHT_4=386,22
PRIMER_LEFT_4_TM=59.242
PRIMER_RIGHT_4_TM=59.835
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_05824 Ps_contig_mira-and-tgicl-ass_franssen_40068
SEQUENCE=ATGTTTATATATATGTATATCACTTTGTTTGACCCTTNGTAAACTTGTGGTGTTGAAGTGAGTGGCTGCTATAAATGTTCTAGAGAAGTACTTGTTTAACATTCCACTGCATCTTCTTTGTTATACATCCTCATCTTATAGTTTGAGAGAGAGAGAGAAAGAAAGAGTTGATGGGAAATTGCCAAGCTATTGATACAGCAACTCTTGTAATACAACAACCAAATGGAAAAGAAGAAAGATTATATTGGCCAGTAAGTGCTAGTGAAGTCATGAAAACAAACCCTAATCACTATGTTGCTCTTCTTATCTCCACAACATTATGCACTTCAAAAGATAGAGAAAATTGTTCAAATAAGACTCACAACAACAACAACAACAACAACCCTGTTCGTATTACACGTATCAAGCTTCTTAAACCAACAGATACACTTATGCTTGGTCAAGTTTATAGACTCATCTCAACTCAAGAAGTTACAAAGGGTATGTGGGCAAAGAAACAAGCAAAAATGAAGAGAAGCTCATCAGAATCATCTCAAAAATCAAATCAGGTTAAGGAAAGAATTGACAAAGAAGCTGAAAGGTCTCAACCACCCGAAAACAATAAGGAAAGGAAATCTGAAAGACATGAGTCAAGGACAAAAGGATCAAGTAATGGTGGAAATCATAACAAGTCAAGAACATGGCAACCCTCTTTACAAAGCATCACTGAATCTTCCAGCTGATATCTGTTTTTCTTTTTTCAATCTTACTCACATGTTGAGAAACTATGATTGCTTAGATCTGTGGAAATTTAATTAAATATACCACATTTTAGGGAATCTAGAGAGAAATCATAAAAATCTGTCATGCATAATAATTGTGTATAGTAAAAGTTGACATATAATATTCGATATCAGTTAATGTATTCAGATATTAATCTTCACTCAAATCAAACTTATTATTATCTGTATTATATTCTNATTTCTGTATTTTTTTAATGTATGGTTTCAGACGAATAATATGTCGACATCTTGACT
TARGET=361,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7009
PRIMER_LEFT_PENALTY=0.058853
PRIMER_RIGHT_PENALTY=0.642030
PRIMER_LEFT_SEQUENCE=TTGGCCAGTAAGTGCTAGTGAA
PRIMER_RIGHT_SEQUENCE=TGCCCACATACCCTTTGTAACT
PRIMER_LEFT=244,22
PRIMER_RIGHT=490,22
PRIMER_LEFT_TM=59.941
PRIMER_RIGHT_TM=60.642
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=247
PRIMER_PAIR_PENALTY_1=0.7511
PRIMER_LEFT_1_PENALTY=0.058853
PRIMER_RIGHT_1_PENALTY=0.692285
PRIMER_LEFT_1_SEQUENCE=TTGGCCAGTAAGTGCTAGTGAA
PRIMER_RIGHT_1_SEQUENCE=GCCCACATACCCTTTGTAACTT
PRIMER_LEFT_1=244,22
PRIMER_RIGHT_1=489,22
PRIMER_LEFT_1_TM=59.941
PRIMER_RIGHT_1_TM=59.308
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=0.8092
PRIMER_LEFT_2_PENALTY=0.058853
PRIMER_RIGHT_2_PENALTY=0.750366
PRIMER_LEFT_2_SEQUENCE=TTGGCCAGTAAGTGCTAGTGAA
PRIMER_RIGHT_2_SEQUENCE=CTTGTTTCTTTGCCCACATACC
PRIMER_LEFT_2=244,22
PRIMER_RIGHT_2=500,22
PRIMER_LEFT_2_TM=59.941
PRIMER_RIGHT_2_TM=60.750
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.8000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.9455
PRIMER_LEFT_3_PENALTY=0.058853
PRIMER_RIGHT_3_PENALTY=0.886637
PRIMER_LEFT_3_SEQUENCE=TTGGCCAGTAAGTGCTAGTGAA
PRIMER_RIGHT_3_SEQUENCE=GCTTGTTTCTTTGCCCACATAC
PRIMER_LEFT_3=244,22
PRIMER_RIGHT_3=501,22
PRIMER_LEFT_3_TM=59.941
PRIMER_RIGHT_3_TM=60.887
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=1.0433
PRIMER_LEFT_4_PENALTY=0.058853
PRIMER_RIGHT_4_PENALTY=0.984414
PRIMER_LEFT_4_SEQUENCE=TTGGCCAGTAAGTGCTAGTGAA
PRIMER_RIGHT_4_SEQUENCE=TTGCCCACATACCCTTTGTAAC
PRIMER_LEFT_4=244,22
PRIMER_RIGHT_4=491,22
PRIMER_LEFT_4_TM=59.941
PRIMER_RIGHT_4_TM=60.984
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_05825 Ps_contig_mira-and-tgicl-ass_franssen_40076
SEQUENCE=CGTAGGAAANAACGAGTATTGATTCAGCCCACTCATATACATGAATATTATTTGGGCGACAACAAGGGAAACAATGTCAAACTTAAGAGGAAACTTAAAGCTAATTTTTCTAGTACACATGGATGTCAACACTAATTCCTCCCAAGTGATTCCTTCTGATGGAGCAACTGCTTTCTCTGCTTAGTTTTTCTGCAGAAATCTTCTGGTCGGTACCATATCGCAGCCAAAGGCATGATATTCTTGCAAATGCGGCCTCTCCAGCAACTCGCACACGATAAACAACACTCAAATCTGCCCATATTTCACTTGACATGGTTACTGAATGAGAATTTGGGTCGTATCCTTCAAGATCTTTCTCTGTTACCACAACTACAGAATTGTCATCAAATTTTACAAGATCTTGTGAAGCTGCTGAGACCTCAGAAGCCTTTACTAATAGATCAGCTACAGGCGAAAATCCAACAACCCTCCTCACAATAAGGTGGCGGTAGTAGTCCTGGACAGCTTGCCACTGAGAGGAAGCTAAAAGCACCCTTGGCCGAATTGACTTTACATATTCATATGCAGCATCAGGTGACATCAGTTTGTGGTGCACCAGATAACAAATAACAATAGTTGTGCTGCGACCCCGTCCAGCTTTGCAGTGGACATAGGTAGTACGTCCAGACAATGCATTTTTATGTATGAAATCAACAGCGCGGCATATGTCATTTAGTGGTGGAGCAAAACAGTAATCTCTAGTAGGAATCACCAGATGATCAATCCCATGAGCATAATATAATGTGGTTGGAACTAAAGTCTCGTATGACTCATTCAATGTGATGACGCCACGAACACCAAGATCTTTCAAGCGAGGAACATCAATAGGAAATGGAACCGCACCTAACAATACAAACTCGTCAACCTTATCCCACCATCGAAACTCAGCCTGAAGCCTATTCCTAACGACATTATAAACAAGCGTGGGATAGAATAGCGCTCGAGCGCCTGCTCCAACCAAAACCCTCTTCGTATCGGAACCAACAAAGCTCCATTTCCCTTCCTCTCCACCTTCTTCAACCTCGCCTCCTTTCAATTCCTCTATACGCATAACTATCTTCCAAATCAGATCAACAATTACAATTTACAATAACCTAATTCACAAATTGTAACCAGTTATCAACACCCCCTTCACGTATCCAACCCACGGTCCTATTGCAATAACCAAAATAATAACAAAAAAATTACAGAAGCAGAGGAACAAAAGAGAAATTGGATTGTCCCGAATTGAAATTTGGGGATCACGGGAAACCTAGAAATTCGGAATTCTACAACAAGAGCTAGAACGAGGAAAACGAGAAAGGAAAAGAGATTGATTCATGCAATAGAAAGAGGAATTTTCCATCATGACTCAGAAAGAATTGATCGGGTTAAGAAAATGGATGTAAAAAGCTGCAAGCGAAAAGAAAAAAAGAGGGAAGAAAATTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAG
TARGET=1467,36
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05826 Ps_contig_mira-and-tgicl-ass_franssen_40078
SEQUENCE=TGGAAAAGTAAATAGAAGTGGTGGTGGTAAAGTAAGTTAAGGGACGAAGGGGGGTGGGGTTAAAGGTTAAAAACCCGTAAACCCCAAACCCTTTACGCAAGGTCTTCTTTATTCTCACCGAAACAACAACAACACACCTCGTGTCACTTCTTTTTCTTTTGCGACATAAGTTGTTTGATTCTGATGTTGATGCATGCACCCTTACAATGTTACATTACGTAATGCACGGCAAGGGTATTCCGTTTTTAATGTTTCTTTGTTTGTTTCTTCAATTCTCGAGACTCAAATAGTGTGTTTTCTTGTTTTTTCCTCTCTCTTCTATAATGTGCTCGTTTTTTGTTTCATGAATTTGTAACTTGTAACACATCATGTAGCCAAGTTGAGCTACTTCTATACTGTATTTATGTTTGTTTTCTTCTCTAAAACTGTCTACCAAAGTACTAGTTACTATAGTTAAAACAGTTTGGAGGACAGGCAATGTTTTCATTGTTA
TARGET=122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0406
PRIMER_LEFT_PENALTY=0.104882
PRIMER_RIGHT_PENALTY=0.935744
PRIMER_LEFT_SEQUENCE=AGTAAGTTAAGGGACGAAGGGG
PRIMER_RIGHT_SEQUENCE=GTGCATGCATCAACATCAGAAT
PRIMER_LEFT=30,22
PRIMER_RIGHT=198,22
PRIMER_LEFT_TM=59.895
PRIMER_RIGHT_TM=60.936
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=1.2180
PRIMER_LEFT_1_PENALTY=0.104882
PRIMER_RIGHT_1_PENALTY=1.113098
PRIMER_LEFT_1_SEQUENCE=AGTAAGTTAAGGGACGAAGGGG
PRIMER_RIGHT_1_SEQUENCE=GAAAAAGAAGTGACACGAGGTG
PRIMER_LEFT_1=30,22
PRIMER_RIGHT_1=155,22
PRIMER_LEFT_1_TM=59.895
PRIMER_RIGHT_1_TM=58.887
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=126
PRIMER_PAIR_PENALTY_2=1.2364
PRIMER_LEFT_2_PENALTY=0.300648
PRIMER_RIGHT_2_PENALTY=0.935744
PRIMER_LEFT_2_SEQUENCE=GGGTGGGGTTAAAGGTTAAAAA
PRIMER_RIGHT_2_SEQUENCE=GTGCATGCATCAACATCAGAAT
PRIMER_LEFT_2=51,22
PRIMER_RIGHT_2=198,22
PRIMER_LEFT_2_TM=60.301
PRIMER_RIGHT_2_TM=60.936
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=1.2652
PRIMER_LEFT_3_PENALTY=0.104882
PRIMER_RIGHT_3_PENALTY=1.160360
PRIMER_LEFT_3_SEQUENCE=AGTAAGTTAAGGGACGAAGGGG
PRIMER_RIGHT_3_SEQUENCE=AGAAAAAGAAGTGACACGAGGTG
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=156,23
PRIMER_LEFT_3_TM=59.895
PRIMER_RIGHT_3_TM=59.840
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=127
PRIMER_PAIR_PENALTY_4=1.2921
PRIMER_LEFT_4_PENALTY=0.104882
PRIMER_RIGHT_4_PENALTY=1.187227
PRIMER_LEFT_4_SEQUENCE=AGTAAGTTAAGGGACGAAGGGG
PRIMER_RIGHT_4_SEQUENCE=TAAAAACGGAATACCCTTGCC
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=247,21
PRIMER_LEFT_4_TM=59.895
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=218
=
PRIMER_SEQUENCE_ID=SSR_05827 Ps_contig_mira-and-tgicl-ass_franssen_40092
SEQUENCE=CGATNCGCATCAATTCTCACCCACACCACACCCTTTGATTCCCCGATCCACGCTGCACCTTCTTCCATTCAATTCCTTCTTTTATTTTCCTCTCCCTTTTTCTCCCTCAATTGAACTCTATTCTATTCTTTTCCCAGATAATTGATATGATTGTTGTTGCTGGAGTTTCCTGAATTGATCTCTTATGCAATTGAAGTTAATTTGCCTATTCAGGTCAATATTTGGTCAATAGTAGTCAGAATTTTGTTTTTCGCGGATGGGTTCATTCCACTTCGAAGCTTTTCTCTAGATTAAAGGAAAAACTATACTGTCATAAGGTTGTGGAATTCTTTTAATGGGTGCTCCTAAGCAAAAATGGACTGCAGAAGAAGAGGCAGCTCTGAAAGCCGGAGTAGTCAAATACGGGGCTGGAAAATGGCGAACCATTCTTACCGACCCGGATTTCAGCACCATTTTGCGAATGCGTTCAAATGTAGATCTCAAGGATAAATGGAGGAACATAAATGTGACAGCAATATGGGGGTCCAGGCAGAAAGCAAAACTTGCACTTAAAAAGAACCTACCTCCTCCTAAATCTGAAAATAACCACGTAACCTCGAATGCCATAGTTCCGTATAATCAAGAAGTTGCTGCTGCTAAGCCTCTAACAGTTTCTGGTGGAACATCTACTTCCAAAGAACAAATACCAAGATTGGACAACCTTATATTAGAGGCTATTGTCAAGTTGAAGGAGCCAAAGGGTTCTGACATTTCCACCATTGCTGCTTTTATAGAGGATCAATACCGGTCTCCACCAAACCTCGTAAAGTTACTTTCATCTAAACTGAAGCATATGGTGGCAAGTGGTAAATTAATGAAGGTAAATCAGAAATACAGGATTGCAACAAATTCTTCAAAAATATCTGAGAAAAAAAGATGCTCTTCGTTGATGCTCCTCGAAGGGAGGCCAAAAGATTCTCCAAAAGCAGAGAAGGCTGATGCCAACATCCTTTCAAAATCCCAAATTGATGTCGAGCTTTTAAAAATGAAAGGTGTGACAGCTCAAGAGGTGGCAGCAGCAGCTGCAAAAGCAGTTGCAGAGGCAGAAGCTGCCATTGCTGAGGCTGAGACAGCAGCACGAGAGGCAGAGGCTGCAGAAGCCGAAGCAGAGGCTGCTCGAGTGTTTGCAAAAGCAGCACTGAAGGCACTAAAATGCAGAAGACTTAATATTTGATGGTGTTTGCTTCTTCGTGGTTGACATGAGCAAAGCAGCAGCAGCATATATTGGCTAGGGTTTGCATGTATTTAATTACTGTGCATAATTTAGGCTTGTAAATTTGGAATTTGTTGGACTGATCCATGAAGCATTTTTGTCGGGAATAGTTTATTTTTTATTAATTTTTGTAATTTTATTTTTTAATGAAAGACACAATGCGGCTATGGTACACGATAATAGTGTTTGGTTATGTAGTGAAAAAAATTTGATTTTGAATGAAATAATTCTTTNAAATTGACTCTATGTAAGATTGAAAAAGTATTGATCGG
TARGET=1247,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0881
PRIMER_LEFT_PENALTY=0.058853
PRIMER_RIGHT_PENALTY=0.029253
PRIMER_LEFT_SEQUENCE=CACTGAAGGCACTAAAATGCAG
PRIMER_RIGHT_SEQUENCE=CTTCATGGATCAGTCCAACAAA
PRIMER_LEFT=1175,22
PRIMER_RIGHT=1343,22
PRIMER_LEFT_TM=59.941
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=0.0881
PRIMER_LEFT_1_PENALTY=0.058853
PRIMER_RIGHT_1_PENALTY=0.029253
PRIMER_LEFT_1_SEQUENCE=CAGCACTGAAGGCACTAAAATG
PRIMER_RIGHT_1_SEQUENCE=CTTCATGGATCAGTCCAACAAA
PRIMER_LEFT_1=1172,22
PRIMER_RIGHT_1=1343,22
PRIMER_LEFT_1_TM=59.941
PRIMER_RIGHT_1_TM=59.971
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=0.0890
PRIMER_LEFT_2_PENALTY=0.058853
PRIMER_RIGHT_2_PENALTY=0.030114
PRIMER_LEFT_2_SEQUENCE=CAGCACTGAAGGCACTAAAATG
PRIMER_RIGHT_2_SEQUENCE=CTATTCCCGACAAAAATGCTTC
PRIMER_LEFT_2=1172,22
PRIMER_RIGHT_2=1361,22
PRIMER_LEFT_2_TM=59.941
PRIMER_RIGHT_2_TM=59.970
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=190
PRIMER_PAIR_PENALTY_3=0.0890
PRIMER_LEFT_3_PENALTY=0.058853
PRIMER_RIGHT_3_PENALTY=0.030114
PRIMER_LEFT_3_SEQUENCE=CACTGAAGGCACTAAAATGCAG
PRIMER_RIGHT_3_SEQUENCE=CTATTCCCGACAAAAATGCTTC
PRIMER_LEFT_3=1175,22
PRIMER_RIGHT_3=1361,22
PRIMER_LEFT_3_TM=59.941
PRIMER_RIGHT_3_TM=59.970
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.1190
PRIMER_LEFT_4_PENALTY=0.058853
PRIMER_RIGHT_4_PENALTY=0.060196
PRIMER_LEFT_4_SEQUENCE=CACTGAAGGCACTAAAATGCAG
PRIMER_RIGHT_4_SEQUENCE=ATGGATCAGTCCAACAAATTCC
PRIMER_LEFT_4=1175,22
PRIMER_RIGHT_4=1339,22
PRIMER_LEFT_4_TM=59.941
PRIMER_RIGHT_4_TM=60.060
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_05828 Ps_contig_mira-and-tgicl-ass_franssen_40098
SEQUENCE=CTCTTGCAAAGACTGTTGAATCCGTATCTCCGCCTCGACAGTGCTCTTGAGATTTCTTATATGTTGCTTAGAGAACTTCAGAAGCCCTAATATCATCAGCCCTTATTTCAACAACCTTATTGGCCAATCCCAATAACAACCTAGAATACTTAGGCACAGTACAATACTTATGCAAGAAAGCCAAAAGCAGTTCCAGGTTCACCAAATCCAAATCAGAAACACACCTCAAAAGTTTCTTCCTAGACACCAATTCCTCCATCACCGCAACCACATAGCCAGGATTCTTACCTTCCAACACACACACTAGAGCCTCCCCGTGCCTAAACTTGTTCAAGAGCTTATCATGTGCTGTCAACTTCACTTTCTTTGGCCTCATAACCAAGTAATCACCCTCAGATGGTTTCTCTCCTTGCCCTCTTTGAAAATACCTATAGTAATTACTTGTCAAAACCCTCTTCTCATTACTCTCCACAGGCCTAATTCTCCAAAATGCACTATCCTCAGCCTTCTTTATCTTCCTCTTCTCAGTCCCGCCAATCTCAGTCTCCTCAATCTCGGTCTCCTTTATCTCAGTCCCCTTTATCTTCCTCTTTGCAGCATAAATTATTCCATTGGAAGTTCCAATCACCCTAGTAGAACAATCTGGCGAATACCCAACAGACAAAAGAGGCGCAGGGAACCTCATTGAATAAGTAACCTTCATAGAACCATAATCAAACACCTTAAAATAACCATCCAAACCAACACTCATTATCCTATATTGTATTCGATTCACTCCC
TARGET=295,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0015
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=TCAGCCCTTATTTCAACAACCT
PRIMER_RIGHT_SEQUENCE=GGTTATGAGGCCAAAGAAAGTG
PRIMER_LEFT=95,22
PRIMER_RIGHT=379,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=285
PRIMER_PAIR_PENALTY_1=0.0015
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=TCAGCCCTTATTTCAACAACCT
PRIMER_RIGHT_1_SEQUENCE=TGGTTATGAGGCCAAAGAAAGT
PRIMER_LEFT_1=95,22
PRIMER_RIGHT_1=380,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=0.0090
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.000742
PRIMER_LEFT_2_SEQUENCE=CCAAATCAGAAACACACCTCAA
PRIMER_RIGHT_2_SEQUENCE=GGTTATGAGGCCAAAGAAAGTG
PRIMER_LEFT_2=207,22
PRIMER_RIGHT_2=379,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.001
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.0090
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=CCAAATCAGAAACACACCTCAA
PRIMER_RIGHT_3_SEQUENCE=TGGTTATGAGGCCAAAGAAAGT
PRIMER_LEFT_3=207,22
PRIMER_RIGHT_3=380,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.0894
PRIMER_LEFT_4_PENALTY=0.088662
PRIMER_RIGHT_4_PENALTY=0.000742
PRIMER_LEFT_4_SEQUENCE=TAGCCAGGATTCTTACCTTCCA
PRIMER_RIGHT_4_SEQUENCE=GGTTATGAGGCCAAAGAAAGTG
PRIMER_LEFT_4=272,22
PRIMER_RIGHT_4=379,22
PRIMER_LEFT_4_TM=60.089
PRIMER_RIGHT_4_TM=60.001
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_05829 Ps_contig_mira-and-tgicl-ass_franssen_40122
SEQUENCE=ATCTTAATTGCGATTATATAGATCCACTTAATATCAGAGGGCTTATATTGTCGCAACCGTTTTTCGGTGGGACCAAGAGAGTACCATCGGAGCTGAGGCTGATAAACGATGCCGTTTTGCCGCCGTATGTGTGCGATTTGATGTGGGAACTATCGTTGCCAATTGGTGTTGACCGTGATCATGAGTATTGTAATCCAACGGTTGGTGATGGTGTTGGGGTTTTAGATGGGGTGAGGGAGCATGGGTTGGAGGGTGCTGGTGAGTGGGTGTGGACGGGGATTCCGTTGATGGACTATCAGATGGGTGGTGGCGAGAGTGATGGAAGAGAAGGGTGTGGTGGTGGTGAAGGTAGTTTACGG
TARGET=334,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05830 Ps_contig_mira-and-tgicl-ass_franssen_40125
SEQUENCE=ATTAGAGAGCAGGTAGACTCGTTTCTTCAAAGCGTTTAACCAACAGCAGGAAGATGAACCAGAAAACGACGCCGTAGTAGAAGATGCGGATAAACCAGAACCAAGCCAAGGTTCTGGTTCAAAGGAAGAAACAGAAGAAGAAGAAGTAGAAGAAGAAGAAGTTGAAGAAGAGGATGATAATGAGAAAGAGGCGAATGAAAAACCTAAGCGAACCAGAAGTGTGAAGAAGGAGAAGAAGAAGAAGAAGAGCACGAAGAGTAAAGAACGATCTAACAAGTCAGGTGATGAGGTAGTAAAGAAAAAAGGTGGCGGCGGTTTTTGTAAAATTTGTAGCCTGTCTCCACAACTTCAGGAATTCGTTGGAGCACCAGAGATGGCTAGGACTGAGGTTGTTAAGCAATTGTGGGCCTATATTAGGGAGAAAAATTTGCAAGACCCAGACAATAGGCGAAATATCAATTGTGATGAACCACTGCGTGCTCTTTTTGGTGTCAATTCCATCAATATGTTCCAAATGAATAAAGTATTGGCAAAGCATATCTGGCCACTGGACTCAGATGATGTTATCCAGGTGAAGTCGGCACCAAAGGAAAAGCAGAAGCGAAAGAGAGAAGATGATGATTCAGATGAGCCGAAAGGAAAGGAAAAACGGCAGAAGGGAGGTTCAGGAAAAGGTTTTCTTGCTCCACTTCAACTATCTGATGCCCTTACGAAGTTCCTTGGAGAAAGTGAATTATCAAGATCTGACGTCATTAAAAGAATGTGGGATTATATAAAAGGAAACAGCCTTCAGGATCCTTCTGACAAGAGGCAAATAATATGTGACGAAAAGTTGAAAGAACTCTTTGGTGTTGACTCCTTCAATGGCTTCACCGTTACAAAACTTTTGGTGCCTCATTTCATAAAGTCATAGCAGTGAGTTGCTTGCACATTTATACGCGGTGAGCGTTTAGGGTAAATTTTGGTAATTTNTTNTTTATTNAATAATTTGGTTAGGACGT
TARGET=133,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0620
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.061230
PRIMER_LEFT_SEQUENCE=GATAAACCAGAACCAAGCCAAG
PRIMER_RIGHT_SEQUENCE=CGCCTCTTTCTCATTATCATCC
PRIMER_LEFT=88,22
PRIMER_RIGHT=192,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=105
PRIMER_PAIR_PENALTY_1=0.1002
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.099438
PRIMER_LEFT_1_SEQUENCE=GATAAACCAGAACCAAGCCAAG
PRIMER_RIGHT_1_SEQUENCE=CTCAGTCCTAGCCATCTCTGGT
PRIMER_LEFT_1=88,22
PRIMER_RIGHT_1=387,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.1287
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.127927
PRIMER_LEFT_2_SEQUENCE=GATAAACCAGAACCAAGCCAAG
PRIMER_RIGHT_2_SEQUENCE=GTTCGCTTAGGTTTTTCATTCG
PRIMER_LEFT_2=88,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.128
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.1449
PRIMER_LEFT_3_PENALTY=0.016950
PRIMER_RIGHT_3_PENALTY=0.127927
PRIMER_LEFT_3_SEQUENCE=GGAAGATGAACCAGAAAACGAC
PRIMER_RIGHT_3_SEQUENCE=GTTCGCTTAGGTTTTTCATTCG
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=212,22
PRIMER_LEFT_3_TM=59.983
PRIMER_RIGHT_3_TM=60.128
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=8.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.1556
PRIMER_LEFT_4_PENALTY=0.027712
PRIMER_RIGHT_4_PENALTY=0.127927
PRIMER_LEFT_4_SEQUENCE=GAAGATGCGGATAAACCAGAAC
PRIMER_RIGHT_4_SEQUENCE=GTTCGCTTAGGTTTTTCATTCG
PRIMER_LEFT_4=79,22
PRIMER_RIGHT_4=212,22
PRIMER_LEFT_4_TM=59.972
PRIMER_RIGHT_4_TM=60.128
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_05831 Ps_contig_mira-and-tgicl-ass_franssen_40125
SEQUENCE=ATTAGAGAGCAGGTAGACTCGTTTCTTCAAAGCGTTTAACCAACAGCAGGAAGATGAACCAGAAAACGACGCCGTAGTAGAAGATGCGGATAAACCAGAACCAAGCCAAGGTTCTGGTTCAAAGGAAGAAACAGAAGAAGAAGAAGTAGAAGAAGAAGAAGTTGAAGAAGAGGATGATAATGAGAAAGAGGCGAATGAAAAACCTAAGCGAACCAGAAGTGTGAAGAAGGAGAAGAAGAAGAAGAAGAGCACGAAGAGTAAAGAACGATCTAACAAGTCAGGTGATGAGGTAGTAAAGAAAAAAGGTGGCGGCGGTTTTTGTAAAATTTGTAGCCTGTCTCCACAACTTCAGGAATTCGTTGGAGCACCAGAGATGGCTAGGACTGAGGTTGTTAAGCAATTGTGGGCCTATATTAGGGAGAAAAATTTGCAAGACCCAGACAATAGGCGAAATATCAATTGTGATGAACCACTGCGTGCTCTTTTTGGTGTCAATTCCATCAATATGTTCCAAATGAATAAAGTATTGGCAAAGCATATCTGGCCACTGGACTCAGATGATGTTATCCAGGTGAAGTCGGCACCAAAGGAAAAGCAGAAGCGAAAGAGAGAAGATGATGATTCAGATGAGCCGAAAGGAAAGGAAAAACGGCAGAAGGGAGGTTCAGGAAAAGGTTTTCTTGCTCCACTTCAACTATCTGATGCCCTTACGAAGTTCCTTGGAGAAAGTGAATTATCAAGATCTGACGTCATTAAAAGAATGTGGGATTATATAAAAGGAAACAGCCTTCAGGATCCTTCTGACAAGAGGCAAATAATATGTGACGAAAAGTTGAAAGAACTCTTTGGTGTTGACTCCTTCAATGGCTTCACCGTTACAAAACTTTTGGTGCCTCATTTCATAAAGTCATAGCAGTGAGTTGCTTGCACATTTATACGCGGTGAGCGTTTAGGGTAAATTTTGGTAATTTNTTNTTTATTNAATAATTTGGTTAGGACGT
TARGET=148,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0620
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.061230
PRIMER_LEFT_SEQUENCE=GATAAACCAGAACCAAGCCAAG
PRIMER_RIGHT_SEQUENCE=CGCCTCTTTCTCATTATCATCC
PRIMER_LEFT=88,22
PRIMER_RIGHT=192,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=105
PRIMER_PAIR_PENALTY_1=0.1002
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.099438
PRIMER_LEFT_1_SEQUENCE=GATAAACCAGAACCAAGCCAAG
PRIMER_RIGHT_1_SEQUENCE=CTCAGTCCTAGCCATCTCTGGT
PRIMER_LEFT_1=88,22
PRIMER_RIGHT_1=387,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.1287
PRIMER_LEFT_2_PENALTY=0.000742
PRIMER_RIGHT_2_PENALTY=0.127927
PRIMER_LEFT_2_SEQUENCE=GATAAACCAGAACCAAGCCAAG
PRIMER_RIGHT_2_SEQUENCE=GTTCGCTTAGGTTTTTCATTCG
PRIMER_LEFT_2=88,22
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=60.001
PRIMER_RIGHT_2_TM=60.128
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=125
PRIMER_PAIR_PENALTY_3=0.1449
PRIMER_LEFT_3_PENALTY=0.016950
PRIMER_RIGHT_3_PENALTY=0.127927
PRIMER_LEFT_3_SEQUENCE=GGAAGATGAACCAGAAAACGAC
PRIMER_RIGHT_3_SEQUENCE=GTTCGCTTAGGTTTTTCATTCG
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=212,22
PRIMER_LEFT_3_TM=59.983
PRIMER_RIGHT_3_TM=60.128
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=8.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.1556
PRIMER_LEFT_4_PENALTY=0.027712
PRIMER_RIGHT_4_PENALTY=0.127927
PRIMER_LEFT_4_SEQUENCE=GAAGATGCGGATAAACCAGAAC
PRIMER_RIGHT_4_SEQUENCE=GTTCGCTTAGGTTTTTCATTCG
PRIMER_LEFT_4=79,22
PRIMER_RIGHT_4=212,22
PRIMER_LEFT_4_TM=59.972
PRIMER_RIGHT_4_TM=60.128
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_05832 Ps_contig_mira-and-tgicl-ass_franssen_40125
SEQUENCE=ATTAGAGAGCAGGTAGACTCGTTTCTTCAAAGCGTTTAACCAACAGCAGGAAGATGAACCAGAAAACGACGCCGTAGTAGAAGATGCGGATAAACCAGAACCAAGCCAAGGTTCTGGTTCAAAGGAAGAAACAGAAGAAGAAGAAGTAGAAGAAGAAGAAGTTGAAGAAGAGGATGATAATGAGAAAGAGGCGAATGAAAAACCTAAGCGAACCAGAAGTGTGAAGAAGGAGAAGAAGAAGAAGAAGAGCACGAAGAGTAAAGAACGATCTAACAAGTCAGGTGATGAGGTAGTAAAGAAAAAAGGTGGCGGCGGTTTTTGTAAAATTTGTAGCCTGTCTCCACAACTTCAGGAATTCGTTGGAGCACCAGAGATGGCTAGGACTGAGGTTGTTAAGCAATTGTGGGCCTATATTAGGGAGAAAAATTTGCAAGACCCAGACAATAGGCGAAATATCAATTGTGATGAACCACTGCGTGCTCTTTTTGGTGTCAATTCCATCAATATGTTCCAAATGAATAAAGTATTGGCAAAGCATATCTGGCCACTGGACTCAGATGATGTTATCCAGGTGAAGTCGGCACCAAAGGAAAAGCAGAAGCGAAAGAGAGAAGATGATGATTCAGATGAGCCGAAAGGAAAGGAAAAACGGCAGAAGGGAGGTTCAGGAAAAGGTTTTCTTGCTCCACTTCAACTATCTGATGCCCTTACGAAGTTCCTTGGAGAAAGTGAATTATCAAGATCTGACGTCATTAAAAGAATGTGGGATTATATAAAAGGAAACAGCCTTCAGGATCCTTCTGACAAGAGGCAAATAATATGTGACGAAAAGTTGAAAGAACTCTTTGGTGTTGACTCCTTCAATGGCTTCACCGTTACAAAACTTTTGGTGCCTCATTTCATAAAGTCATAGCAGTGAGTTGCTTGCACATTTATACGCGGTGAGCGTTTAGGGTAAATTTTGGTAATTTNTTNTTTATTNAATAATTTGGTTAGGACGT
TARGET=231,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0936
PRIMER_LEFT_PENALTY=0.061230
PRIMER_RIGHT_PENALTY=0.032347
PRIMER_LEFT_SEQUENCE=GGATGATAATGAGAAAGAGGCG
PRIMER_RIGHT_SEQUENCE=CCACAATTGCTTAACAACCTCA
PRIMER_LEFT=171,22
PRIMER_RIGHT=405,22
PRIMER_LEFT_TM=60.061
PRIMER_RIGHT_TM=60.032
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=11.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=0.1002
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.099438
PRIMER_LEFT_1_SEQUENCE=GATAAACCAGAACCAAGCCAAG
PRIMER_RIGHT_1_SEQUENCE=CTCAGTCCTAGCCATCTCTGGT
PRIMER_LEFT_1=88,22
PRIMER_RIGHT_1=387,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.901
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.1603
PRIMER_LEFT_2_PENALTY=0.127927
PRIMER_RIGHT_2_PENALTY=0.032347
PRIMER_LEFT_2_SEQUENCE=CGAATGAAAAACCTAAGCGAAC
PRIMER_RIGHT_2_SEQUENCE=CCACAATTGCTTAACAACCTCA
PRIMER_LEFT_2=191,22
PRIMER_RIGHT_2=405,22
PRIMER_LEFT_2_TM=60.128
PRIMER_RIGHT_2_TM=60.032
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=215
PRIMER_PAIR_PENALTY_3=0.1607
PRIMER_LEFT_3_PENALTY=0.061230
PRIMER_RIGHT_3_PENALTY=0.099438
PRIMER_LEFT_3_SEQUENCE=GGATGATAATGAGAAAGAGGCG
PRIMER_RIGHT_3_SEQUENCE=CTCAGTCCTAGCCATCTCTGGT
PRIMER_LEFT_3=171,22
PRIMER_RIGHT_3=387,22
PRIMER_LEFT_3_TM=60.061
PRIMER_RIGHT_3_TM=59.901
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=0.1607
PRIMER_LEFT_4_PENALTY=0.061230
PRIMER_RIGHT_4_PENALTY=0.099438
PRIMER_LEFT_4_SEQUENCE=GGATGATAATGAGAAAGAGGCG
PRIMER_RIGHT_4_SEQUENCE=ACCTCAGTCCTAGCCATCTCTG
PRIMER_LEFT_4=171,22
PRIMER_RIGHT_4=389,22
PRIMER_LEFT_4_TM=60.061
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=11.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_05833 Ps_contig_mira-and-tgicl-ass_franssen_40138
SEQUENCE=TCAACGCAGAGGTCTTGTCTCAAGAACAGTGAGTGAACCCACCCACATCTTCTTCCTTTCTCTCTCCCTCTCTCTGGGCTAACAGAAACACTAATTAATTAATTAATGGAGTTTGAGCATGAAGATGTCTTTTCCAAAGAATGGAATTCCATTGGAAGTTCCATGACAGAGACAGAGAAGAACTGTGAGAGTTGTTTTGATTGCAACATCTGTTTAGATTTTGCACATGAACCAGTAGTGACACTCTGTGGTCACCTGTTCTGTTGGTCCTGCATCTACAAATGGCTCTATGTGCAAAGTGCTTCTCTTGCACCTGATGAGCCACCACAGTGTCCAGTTTGCAAACATGAAATATCTCACACCAAAATGGTCCCTCTCTACGGCAGAGGCCAGACCGCGATTCACGACCGGAAAACTACGCTTCAGGATGTTTCTATACCACCAAGGCCACCTGCTTCAGGAATTCAATCTCTGTTTGCAATGGCAACATTGCCTCAAAGGGGTCATCATCAGCAGCAGCAGCTTCCGTATCGTAATCCTTANCAGAGTCAAAAATACTAA
TARGET=92,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5523
PRIMER_LEFT_PENALTY=0.384229
PRIMER_RIGHT_PENALTY=0.168114
PRIMER_LEFT_SEQUENCE=CACCCACATCTTCTTCCTTTCT
PRIMER_RIGHT_SEQUENCE=TTTGTAGATGCAGGACCAACAG
PRIMER_LEFT=39,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=59.616
PRIMER_RIGHT_TM=60.168
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=243
PRIMER_PAIR_PENALTY_1=0.7300
PRIMER_LEFT_1_PENALTY=0.384229
PRIMER_RIGHT_1_PENALTY=0.345764
PRIMER_LEFT_1_SEQUENCE=CACCCACATCTTCTTCCTTTCT
PRIMER_RIGHT_1_SEQUENCE=GAACAGGTGACCACAGAGTGTC
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=260,22
PRIMER_LEFT_1_TM=59.616
PRIMER_RIGHT_1_TM=59.654
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=222
PRIMER_PAIR_PENALTY_2=0.8855
PRIMER_LEFT_2_PENALTY=0.384229
PRIMER_RIGHT_2_PENALTY=0.501224
PRIMER_LEFT_2_SEQUENCE=CACCCACATCTTCTTCCTTTCT
PRIMER_RIGHT_2_SEQUENCE=CATTTGTAGATGCAGGACCAAC
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=283,22
PRIMER_LEFT_2_TM=59.616
PRIMER_RIGHT_2_TM=59.499
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=1.0005
PRIMER_LEFT_3_PENALTY=0.384229
PRIMER_RIGHT_3_PENALTY=0.616243
PRIMER_LEFT_3_SEQUENCE=CACCCACATCTTCTTCCTTTCT
PRIMER_RIGHT_3_SEQUENCE=TAGAGCCATTTGTAGATGCAGG
PRIMER_LEFT_3=39,22
PRIMER_RIGHT_3=289,22
PRIMER_LEFT_3_TM=59.616
PRIMER_RIGHT_3_TM=59.384
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=1.0419
PRIMER_LEFT_4_PENALTY=0.873742
PRIMER_RIGHT_4_PENALTY=0.168114
PRIMER_LEFT_4_SEQUENCE=CTCTCCCTCTCTCTGGGCTAAC
PRIMER_RIGHT_4_SEQUENCE=TTTGTAGATGCAGGACCAACAG
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=281,22
PRIMER_LEFT_4_TM=60.874
PRIMER_RIGHT_4_TM=60.168
PRIMER_LEFT_4_GC_PERCENT=59.091
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_05834 Ps_contig_mira-and-tgicl-ass_franssen_40138
SEQUENCE=TCAACGCAGAGGTCTTGTCTCAAGAACAGTGAGTGAACCCACCCACATCTTCTTCCTTTCTCTCTCCCTCTCTCTGGGCTAACAGAAACACTAATTAATTAATTAATGGAGTTTGAGCATGAAGATGTCTTTTCCAAAGAATGGAATTCCATTGGAAGTTCCATGACAGAGACAGAGAAGAACTGTGAGAGTTGTTTTGATTGCAACATCTGTTTAGATTTTGCACATGAACCAGTAGTGACACTCTGTGGTCACCTGTTCTGTTGGTCCTGCATCTACAAATGGCTCTATGTGCAAAGTGCTTCTCTTGCACCTGATGAGCCACCACAGTGTCCAGTTTGCAAACATGAAATATCTCACACCAAAATGGTCCCTCTCTACGGCAGAGGCCAGACCGCGATTCACGACCGGAAAACTACGCTTCAGGATGTTTCTATACCACCAAGGCCACCTGCTTCAGGAATTCAATCTCTGTTTGCAATGGCAACATTGCCTCAAAGGGGTCATCATCAGCAGCAGCAGCTTCCGTATCGTAATCCTTANCAGAGTCAAAAATACTAA
TARGET=511,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.2970
PRIMER_LEFT_PENALTY=0.136017
PRIMER_RIGHT_PENALTY=2.160971
PRIMER_LEFT_SEQUENCE=CACCAAAATGGTCCCTCTCTAC
PRIMER_RIGHT_SEQUENCE=TCTGNTAAGGATTACGATACGGA
PRIMER_LEFT=359,22
PRIMER_RIGHT=546,23
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=58.839
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=2.3291
PRIMER_LEFT_1_PENALTY=0.168114
PRIMER_RIGHT_1_PENALTY=2.160971
PRIMER_LEFT_1_SEQUENCE=CTGTTGGTCCTGCATCTACAAA
PRIMER_RIGHT_1_SEQUENCE=TCTGNTAAGGATTACGATACGGA
PRIMER_LEFT_1=260,22
PRIMER_RIGHT_1=546,23
PRIMER_LEFT_1_TM=60.168
PRIMER_RIGHT_1_TM=58.839
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=2.3930
PRIMER_LEFT_2_PENALTY=0.232060
PRIMER_RIGHT_2_PENALTY=2.160971
PRIMER_LEFT_2_SEQUENCE=GTTTCTATACCACCAAGGCCAC
PRIMER_RIGHT_2_SEQUENCE=TCTGNTAAGGATTACGATACGGA
PRIMER_LEFT_2=429,22
PRIMER_RIGHT_2=546,23
PRIMER_LEFT_2_TM=59.768
PRIMER_RIGHT_2_TM=58.839
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.75
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=2.4213
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=2.285317
PRIMER_LEFT_3_SEQUENCE=CACCAAAATGGTCCCTCTCTAC
PRIMER_RIGHT_3_SEQUENCE=CTCTGNTAAGGATTACGATACGGA
PRIMER_LEFT_3=359,22
PRIMER_RIGHT_3=547,24
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.715
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=2.4534
PRIMER_LEFT_4_PENALTY=0.168114
PRIMER_RIGHT_4_PENALTY=2.285317
PRIMER_LEFT_4_SEQUENCE=CTGTTGGTCCTGCATCTACAAA
PRIMER_RIGHT_4_SEQUENCE=CTCTGNTAAGGATTACGATACGGA
PRIMER_LEFT_4=260,22
PRIMER_RIGHT_4=547,24
PRIMER_LEFT_4_TM=60.168
PRIMER_RIGHT_4_TM=59.715
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_05835 Ps_contig_mira-and-tgicl-ass_franssen_40146
SEQUENCE=CCTNAAAACCCCAACGTAACTATAATAGCCACGTGATCCAATACCAAAGACCACAAAGACTTAGCAGCAGCAACACTCAAAGACTGCAAAACGACAGCTGCTACGACATGAAAAAGACTGCAAAATAGCAGCAGAAAAAACAAAAATAACAACGCCAAAAATGAAAACGCAGCAAGCTACCACCCAACGCGCTGTTATCCATGAGCCACTTGCAAAAACTCCAAAAGCTGCAACAAAGCTACAAACAGCTTCCAAAGCGTGAGTCAAGCCAATGACGCAAGCAACACGCGACCATAACGCGGCAACAACTCCAAAACGGCAATGCAAATGCAACCTCCAAAACGTATACCAGCTAACCACCTGCAACAACAACTTGAAACACAGAAAAAGATAGAAACGGAAGCTAACACAGTGAAACGCGAGCCACCAAGCTGCGACTTGAGCGGAATCCTGTAAACGACTCCAAGAATCAAAACGCAGCAATACAGTGAGTGAAACACAGCTCGTAAGAAACTCAAAATGCAGAAGATTAACAGAATGTGATCCCCTATTTAAACAAGAGTTTCCCTTCAGTTTTCATTTGGGCTCACTATTCTTCGACCTGCAGAATTGATTACTCACGATTTACGCTGCTCCCACAATTCATCTTCACTCATCATCGATCAAATTCTCAAGTCTCACGAACTCTCAATTAAACTAACCTGCACAATTTTTCCATAATCCGTCACTGCAGTGCATCGTACTCGCTCATCGTCTTCACGCGGTTTCCCTCTGCRTTGACTACCATTTCTTCATTCTCCCGATACCTTGCGCACATAGCTGAAGAAGAATAGAAGAGGAGTAGAAGTAACTTGAACGAAATAGAGGTTCAGGAGCACAGAGTTCGACCTCTGCCGACGAGTTCACCTTCGCCGTCGCCGGTAGGTTYCTCGTCTTCTCACGATTTTTCGATCTAATCGTGATGGTGATGCGAATCTTGTCTCGCGGAAGCATAACCTTGTAGTCCTTGGTCGAGTCACGCTCCGTCGTTGTTCGGCGTTTGATTCATCCGTTGTGAGGATCGTGCGTTATCGTTTGGCTGTGGTTTGTGGATAAGTTCGTTTATGGAGTCATTTAGGAAAGTTTGAGACGGTAGTAGCGGTTGTAGGGAGGTGAGCGTGAGGCGATTGAGGGCTCCGCCGCCGCCGCCGACAATGAGCTCCGGCGCGGCGGAGTCGTTAGGTGATCGCCGGCCATGTTTTCAAGTNGGAGAG
TARGET=1176,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05836 Ps_contig_mira-and-tgicl-ass_franssen_40153
SEQUENCE=AGAGAAGATGNTGAAGTTGTTATTGGAAAAGTCCCTATATATGGTTCTGATGATTCGGAATCTCCTTATCTACTTGCTGGTTTCCATGGAAAACTTCATGTCATCACAAAAGATGCCAATCATAATATTTCTGTTCTGCAAGCAGAGCTGCGCAATAATTTGAATTCTTCACCGTCGTCGTCGTCGTCCTCAACTCTGTCACAAAGTTCATTGCATGAATCCGTGGACCCGCCTGTCGAAACAGATGCAGTAGTTTGGAAAATAATTGCAAGTAGGTGTTTGGGACAGGTTGAACTAGTTAGTTGTCAAGTTATTGACATGTAGCTGCAAAAAGGCAAGCAATAAATGTGACAGAAAATCTGTGAAGGGTTAGTTACCTGGTAGGACTGAGTGAGTACATTTCGACATTGATTAAGGTGCCCGCGAATTGAAGAGTGCTGTTATAGTTTGGTTGTAGCTATTGTTCTTTTACGTAAATAAGTTCTATAGGAACTGTCAGATTGTACACTGACACGATAGAATAATATCAGAGAAAGAAATCCATTATATCCACTCTTTGTCATTCTACATA
TARGET=173,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6254
PRIMER_LEFT_PENALTY=0.561317
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=GTCATCACAAAAGATGCCAATC
PRIMER_RIGHT_SEQUENCE=CTACTGCATCTGTTTCGACAGG
PRIMER_LEFT=99,22
PRIMER_RIGHT=252,22
PRIMER_LEFT_TM=59.439
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.6994
PRIMER_LEFT_1_PENALTY=0.561317
PRIMER_RIGHT_1_PENALTY=0.138114
PRIMER_LEFT_1_SEQUENCE=GTCATCACAAAAGATGCCAATC
PRIMER_RIGHT_1_SEQUENCE=TCTGTCACATTTATTGCTTGCC
PRIMER_LEFT_1=99,22
PRIMER_RIGHT_1=354,22
PRIMER_LEFT_1_TM=59.439
PRIMER_RIGHT_1_TM=60.138
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.7716
PRIMER_LEFT_2_PENALTY=0.707509
PRIMER_RIGHT_2_PENALTY=0.064105
PRIMER_LEFT_2_SEQUENCE=CCATGGAAAACTTCATGTCATC
PRIMER_RIGHT_2_SEQUENCE=CTACTGCATCTGTTTCGACAGG
PRIMER_LEFT_2=83,22
PRIMER_RIGHT_2=252,22
PRIMER_LEFT_2_TM=59.292
PRIMER_RIGHT_2_TM=59.936
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.8456
PRIMER_LEFT_3_PENALTY=0.707509
PRIMER_RIGHT_3_PENALTY=0.138114
PRIMER_LEFT_3_SEQUENCE=CCATGGAAAACTTCATGTCATC
PRIMER_RIGHT_3_SEQUENCE=TCTGTCACATTTATTGCTTGCC
PRIMER_LEFT_3=83,22
PRIMER_RIGHT_3=354,22
PRIMER_LEFT_3_TM=59.292
PRIMER_RIGHT_3_TM=60.138
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=272
PRIMER_PAIR_PENALTY_4=0.8761
PRIMER_LEFT_4_PENALTY=0.561317
PRIMER_RIGHT_4_PENALTY=0.314775
PRIMER_LEFT_4_SEQUENCE=GTCATCACAAAAGATGCCAATC
PRIMER_RIGHT_4_SEQUENCE=CAACCTGTCCCAAACACCTACT
PRIMER_LEFT_4=99,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=59.439
PRIMER_RIGHT_4_TM=60.315
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=5.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_05837 Ps_contig_mira-and-tgicl-ass_franssen_40155
SEQUENCE=ATCCTGCCTTCAAAAACAACTCTCTCTCTATAANCRNRGACTCTCCTTTTTATACCCTTCATCTATAAATTGAACCGATGATGAAACAACACTGTACCTGAACCTTCTTCAGGACCATTTCTCATTTCTACACACAAAGTATTAACTCTTTTGCCGGCTTACATATCAATTTTGAAGGAGTTCATTCAATACTTGGCGGTGTTTTTCGGTTCCTTGTAATTTGTGCTACCATGTCATTATTGCCTAAACTACGATGTGTCACTCTAGATGTTACCGGCACCCTGATGGCTTACAGAGGGGAGCTTGGTGACTATTATTGCATGGCTGCCAAAGCTTCTGGCCGTCCATGCCCAGACTACAAGCGTATGCACGAGGGATTTAAGTATGCATATAAGGACATGGTGAGGAAGTATCCGTGTTTTGGGTTTGCGGCCAAAATGCCAAACATTGTGTGGTGGAAAGCCTGCGTGCGAGATTCCTTTGTCAGGGCTGGATATGAGTACGACGAGGAGACATTTGAAAAGATTTTCAAGCGCATCTATGCATCCTTTGGTTCATCTGCTCCATATACTGTCTTTCCAGATTCTAAGCCATTTCTTAGATGGCTTCGTGGAAAGGGTCTCAAGGTTGGGGTTGTTAGCAATGCTGAGTATCGATATCGAGATGTGATTCTTCCAGCTTTAGGCTTAAATCAGGGAACTGAGTGGGACTTTGGTGTTTTTTCTGGCCTTGAAGGCGTTGAGAAACCAGACCCAAGAATTTACGAGATTGCACTCGAGAGGGCTGGAAATATTGCACCTGAAGAAACTTTACATATTGGCGATAGCTTCCGCAAAGACTATGAGCCAGCTAAGAGCATAGGGATGCATGCACTATTGTTGGATAGATTTAAGACACCAGAAGCCGAGGAGTGGAGGAAATCCGGGGCTGTTGTTCTTCCCGACTTAAATGCAACACAAGAATGGCTGTCTTCAGAGAATTCAACTTGCTAGAGGTTCTACATCTTTGCTGTAGTAGTATATTTACGTATCGTTTTGCTCATCTGATAACATACCTGCTATCTATACCTTTGACGGGTTTCATAAAGGCATGTGTCTGAATGTTGACGTGCAGAGCCATGATTGTGGATTTCTGTTGGCTCCTGTGATCTTCTGATGTAGCTCTACCATAACTTTGTAATACAATTATATTAAAATGCTACTTTCTCTTCATCGGTCRCTTAGTTTCCAATATATTTTACTTTCTCAGTTGCACATGACAACGGGCGATATCGAATATGGATACTTCTTAA
TARGET=20,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05838 Ps_contig_mira-and-tgicl-ass_franssen_40162
SEQUENCE=AACGTTGGGAACGTTGAGCGCAGTGGTGGGGGTTACGAAATAAAGAAAGAAAGGGTACGAAAAGAANNAAGGACAGAAAAGTAGGAGGGAAGGAACCTCCAGTAAAGTTAGAGGAAGAAACTTGATCTGCAGATAACAGGAACCGAAAGGCCCTCCATATGGAAACTTATTGGTGTCCGCATCATTCTTTTACCTTATACTTTAGGAAAGCTGCTGCTATGGTCTTCCTGTTGGTTTTGGACATACAAGGTGAAAAAGTCCCCTTATTCTTGGGAAGATGCTTCTTATCTCACACAAAGTTCCCTTGGTCTTNCTGATGACAGATGGAGAAGTATAGATGAAGCAACAAAGGAAGATCTTGTGCTAAGAAGCTTATGGGAGAAGTCAAATATGGAGAACTACATGACAGAGATGCGAAAAGAATCAAAGCGCAGAAGATAGCTCCTACAGTTGCTTCACCTGCTGTTTTCAAAGGGCAAAATTTTGTTTTACTTATCTGAATTACAACGTGATAGTTAAAAGTATAACACTTAAAAATTATTTATTATGGGGTAGCTTTATTAATATTTTGGGGGAGAGGAGAGTAAAATCGATCTGGTCCAACATAAATCTTATATTTATAAACAAGATGTACTAAGTTAGCAACATGAAATTTAAATAGGTTGCTCTTTGACTGTCYGTAAACAGAAAACTGAAGGAAACTGGTTTAGTTATTACTACATTCTTTTAATGTAGGTACTTAACCTCAGTCTTGCAGCCATTGAATGTTTGGCAGTGGTTTTGTGCACCTGTTGTTCAATAAGTATGAGGTGTTTTATCAGAAAGCGGCATGTTGTAGTAGAGTTATGAATTGTGTGTAAAAGATACATGTGARATACCTCTGCTTTTACATTTATTTTTAG
TARGET=42,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05839 Ps_contig_mira-and-tgicl-ass_franssen_40172
SEQUENCE=GATTGAATTGATTGATGATGTTTATCCTCTCCAACGGACACCATTATTGAACTCAACTCCAGTTGTTGTATTTTACCGTTGCAATTCCATTTCCTCAATCACTCAATCACTCACCTTATAAATACACAAAAACCCTAATAAAGTCGAAGAATTCAGAATCACAATTCACATCACTGTAATGGAAAATATGGTGAAAAACCTTCAACAAGTTCCAATTCAAACTCCAATAATCGAGCCTCAATCCACCAAAACACCAACTCCTCCACCACCTCCAGTTCATCATCATCATAAAGCCTCCAATGAATTGCGCAAACCTGTCACTCCAGATCATCTTAAACTCCCCAAAGCATTCAAATATCCAGAAAGGTATACAAGTCCAACTGATATGATGATGTCCCCGGTAACCAAAGGCCTCCTTGCCAGAAATAAAAAGGGTGCTGCAAAGTTACCACCTGGAATTGGAGCTAAATATCAGCCAAAGGTTCCAGAGATGTGCTTGCAGGATGTGAGTACTTTTCAAAACAAGCTTCCAATGATGATTGATGAGAAAATCAACTCAACTTGAGGAATTCAATGGAGTTTTGTTATACATGATGAATTATGTGGTAGTGGTTTCAGTGGAACTTAAAGATGAAGAGTTTTGTTAGAGAGAGCTGTTGTATCTTCTGATTTTTGTTCCTAAATAGGTTTTCTACCTATATATACATTGATGTTAAAGAGTTTTGTACTTTCAATTCTATAGAACCTTAAAGAAACTTGTGTAAAAGAATTCCAATCAGATGATTTTGTTTTACCCTTCTAGTAGAATTGGTTGATCCTTTATTCTTTTTTAATAGATAAATAACTTACGCATAATCGCTTATATGAGAAGTGTATTTATGCTATAAGCACTTAATTCGACTGTT
TARGET=277,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2873
PRIMER_LEFT_PENALTY=0.095445
PRIMER_RIGHT_PENALTY=0.191886
PRIMER_LEFT_SEQUENCE=AAACTCCAATAATCGAGCCTCA
PRIMER_RIGHT_SEQUENCE=GATATTTGAATGCTTTGGGGAG
PRIMER_LEFT=218,22
PRIMER_RIGHT=358,22
PRIMER_LEFT_TM=60.095
PRIMER_RIGHT_TM=59.808
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.3544
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.258908
PRIMER_LEFT_1_SEQUENCE=AAACTCCAATAATCGAGCCTCA
PRIMER_RIGHT_1_SEQUENCE=TCCAATTCCAGGTGGTAACTTT
PRIMER_LEFT_1=218,22
PRIMER_RIGHT_1=462,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=59.741
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.3874
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.291945
PRIMER_LEFT_2_SEQUENCE=AAACTCCAATAATCGAGCCTCA
PRIMER_RIGHT_2_SEQUENCE=GTAACTTTGCAGCACCCTTTTT
PRIMER_LEFT_2=218,22
PRIMER_RIGHT_2=448,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=59.708
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.4072
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.311784
PRIMER_LEFT_3_SEQUENCE=AAACTCCAATAATCGAGCCTCA
PRIMER_RIGHT_3_SEQUENCE=TGGGGAGTTTAAGATGATCTGG
PRIMER_LEFT_3=218,22
PRIMER_RIGHT_3=343,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=60.312
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.4864
PRIMER_LEFT_4_PENALTY=0.095445
PRIMER_RIGHT_4_PENALTY=0.390906
PRIMER_LEFT_4_SEQUENCE=AAACTCCAATAATCGAGCCTCA
PRIMER_RIGHT_4_SEQUENCE=GAATGCTTTGGGGAGTTTAAGA
PRIMER_LEFT_4=218,22
PRIMER_RIGHT_4=351,22
PRIMER_LEFT_4_TM=60.095
PRIMER_RIGHT_4_TM=59.609
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_05840 Ps_contig_mira-and-tgicl-ass_franssen_40174
SEQUENCE=GTTAAGGTTTTAACTAAACTAGCTGGGGTTTCCCGACGTGGCGTTTTAATTAATTCCTTATCTAGTGCCGGAAAGTAATGATTGGCAGGATCACTTGACGGTTATGAGCAATCAAATTTACAAATGTGGTCGTTGTTCCATGGAAAAGNGCCTTTCAGCTACCTGAAAGTGTAGGCTGTTAATGCAGCACGAAATCCGCNAAAAACAGTGGAATCATGATCCTCTGGAACTCTACCATGTTGATTCCTTCCAAAATTTCTTCCAGTTCCCCGAGAAACATCACTTCTTTCTGACTATTTATTACTGGCCAATATTTCAACAGACCACTTATAACAATGCTCGCTAACTTCGGCTCCTTCTCTAAGAACTGCGTAATACAGAAGGACAGTTGCTGAAAGTACACCCCCAAAGATTTAGGCTTGTGTAAGGGGATCAAAACTCTCCACAAGAAGATTTTATGTTCCTCTTTCAAGGGTAATGCAAATCCACAAATCACACTGCCGAAAATCTCCAACAACTCGGCAATCCCGTTATGTTTCTCGGTCTCAATCACAAATCGGTAGAATATATTGTTAATTGTTTTTCGAATAAACGGCCTATGCACCATGAACTTCCCATAAACCCTGTGCAGAATTGTCTTCAAACAGTCTCTTTCTCTTGGATCCTCGGAATCAAACAGTTCAAGCAATTTCGAAATGAACGAATGATCGATATACTTTTTTGCTACCTTAGCATCGAGGCACGAAGATGAGATAAATTTAAGCAGCAATTCATATACAAGTTGCAAATGTGGCCAAGCAGGATCAAACATTGGGTCATCATCATCATTCTCACCACCACTAGCAAAAACTCCATTAACCCTATAATTAGGCGGAAAAACTCTAAACAAATTAATAGCAGACATTCTACACATTGCAAGAATAGCAGGTTCACTAAACTTCATTGATCCACAAGCCACAAAATCAACAAGTTCAACTAAAGTTTTCCTTTTCACATCTTTATCAGCAATGTTCTTACCAGGGTCAGTGAAATCAAATGTCACACAACAAAGACTCAATTTGCTCACATACAGGTTCATCTTTTCAGCATTCGGAACATCCTTAAACGGCACCAAAGGTTCAATCCCAGAAACCATGCTAGCCGGAAACACTGCCGATGAAGAAGCTCGTTTCAACGAAGAAGCGCCACCTTGCTGCCGATTGTGGCATTTACCGGCAACACGTGGAGAATCTCCAACCTGGGTCGACTCATCTTCATCGATTTTGGAAGATTTCCGGGGAAGCTTGTTGAAAAGTTGCTTCAGCATTGCAACCCAGAAGCTGAAATCCACCAATTCCAAAGCTTTTACATCAAACCCATGGAGAAACAGTCAAAATCAACCAAACCCCAAAAAATTACTAATTCTGAACCAGAAAAACCCTAGATCTCGTTCACCTAAACAAATACAAATTTCAAGCAATCTAGATTTCCACCTGAAATTGAGATCCACATTTGAAATGGGGTTCAAGAAATTCTCAATCTTAAAGCATGCATTGATGTAAAAATCAAATCTTGAAGATTGCGGTTCGTGTGTTACGAAGAACACGTGAACCAAAAGAGGAAAGGTAAAACCAAAGGACGACAACCACAACAACAACAAAAAGTTTGTTACAAATAAACAGAACAACAACAATAAACAACAACAAACAATGATTACAATGATAATGTTACATCCGAATCAAATCTCACATTGGAACATTTTTTCCAATTTTTCTTAGGAATATGAAAATAAAAGAAAAATAAACTAANTTTTGGTATATTAATAAATGATGGATCTAGGAAGAAATGAAATGCATAGACTAAAGTTNTTTTTAGGTTAGGTTTTTTGGGGGGAGTAAGGTTAGGGTAATTAAGGTAAAAGTTAAAGGTAAAAGTTAAACGGGTAAAAAACCGGTTAACCCTTTACGTTTAA
TARGET=816,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4137
PRIMER_LEFT_PENALTY=0.053348
PRIMER_RIGHT_PENALTY=0.360320
PRIMER_LEFT_SEQUENCE=GATCCTCGGAATCAAACAGTTC
PRIMER_RIGHT_SEQUENCE=GGCTTGTGGATCAATGAAGTTT
PRIMER_LEFT=660,22
PRIMER_RIGHT=955,22
PRIMER_LEFT_TM=59.947
PRIMER_RIGHT_TM=60.360
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=296
PRIMER_PAIR_PENALTY_1=0.4444
PRIMER_LEFT_1_PENALTY=0.039879
PRIMER_RIGHT_1_PENALTY=0.404566
PRIMER_LEFT_1_SEQUENCE=AAACCCTGTGCAGAATTGTCTT
PRIMER_RIGHT_1_SEQUENCE=ATGGAGTTTTTGCTAGTGGTGG
PRIMER_LEFT_1=618,22
PRIMER_RIGHT_1=854,22
PRIMER_LEFT_1_TM=60.040
PRIMER_RIGHT_1_TM=60.405
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=237
PRIMER_PAIR_PENALTY_2=0.4579
PRIMER_LEFT_2_PENALTY=0.053348
PRIMER_RIGHT_2_PENALTY=0.404566
PRIMER_LEFT_2_SEQUENCE=GATCCTCGGAATCAAACAGTTC
PRIMER_RIGHT_2_SEQUENCE=ATGGAGTTTTTGCTAGTGGTGG
PRIMER_LEFT_2=660,22
PRIMER_RIGHT_2=854,22
PRIMER_LEFT_2_TM=59.947
PRIMER_RIGHT_2_TM=60.405
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.6139
PRIMER_LEFT_3_PENALTY=0.209343
PRIMER_RIGHT_3_PENALTY=0.404566
PRIMER_LEFT_3_SEQUENCE=TATGCACCATGAACTTCCCATA
PRIMER_RIGHT_3_SEQUENCE=ATGGAGTTTTTGCTAGTGGTGG
PRIMER_LEFT_3=597,22
PRIMER_RIGHT_3=854,22
PRIMER_LEFT_3_TM=60.209
PRIMER_RIGHT_3_TM=60.405
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=258
PRIMER_PAIR_PENALTY_4=0.7543
PRIMER_LEFT_4_PENALTY=0.039879
PRIMER_RIGHT_4_PENALTY=0.714453
PRIMER_LEFT_4_SEQUENCE=AAACCCTGTGCAGAATTGTCTT
PRIMER_RIGHT_4_SEQUENCE=GTTTTTGCTAGTGGTGGTGAGA
PRIMER_LEFT_4=618,22
PRIMER_RIGHT_4=849,22
PRIMER_LEFT_4_TM=60.040
PRIMER_RIGHT_4_TM=59.286
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_05841 Ps_contig_mira-and-tgicl-ass_franssen_40174
SEQUENCE=GTTAAGGTTTTAACTAAACTAGCTGGGGTTTCCCGACGTGGCGTTTTAATTAATTCCTTATCTAGTGCCGGAAAGTAATGATTGGCAGGATCACTTGACGGTTATGAGCAATCAAATTTACAAATGTGGTCGTTGTTCCATGGAAAAGNGCCTTTCAGCTACCTGAAAGTGTAGGCTGTTAATGCAGCACGAAATCCGCNAAAAACAGTGGAATCATGATCCTCTGGAACTCTACCATGTTGATTCCTTCCAAAATTTCTTCCAGTTCCCCGAGAAACATCACTTCTTTCTGACTATTTATTACTGGCCAATATTTCAACAGACCACTTATAACAATGCTCGCTAACTTCGGCTCCTTCTCTAAGAACTGCGTAATACAGAAGGACAGTTGCTGAAAGTACACCCCCAAAGATTTAGGCTTGTGTAAGGGGATCAAAACTCTCCACAAGAAGATTTTATGTTCCTCTTTCAAGGGTAATGCAAATCCACAAATCACACTGCCGAAAATCTCCAACAACTCGGCAATCCCGTTATGTTTCTCGGTCTCAATCACAAATCGGTAGAATATATTGTTAATTGTTTTTCGAATAAACGGCCTATGCACCATGAACTTCCCATAAACCCTGTGCAGAATTGTCTTCAAACAGTCTCTTTCTCTTGGATCCTCGGAATCAAACAGTTCAAGCAATTTCGAAATGAACGAATGATCGATATACTTTTTTGCTACCTTAGCATCGAGGCACGAAGATGAGATAAATTTAAGCAGCAATTCATATACAAGTTGCAAATGTGGCCAAGCAGGATCAAACATTGGGTCATCATCATCATTCTCACCACCACTAGCAAAAACTCCATTAACCCTATAATTAGGCGGAAAAACTCTAAACAAATTAATAGCAGACATTCTACACATTGCAAGAATAGCAGGTTCACTAAACTTCATTGATCCACAAGCCACAAAATCAACAAGTTCAACTAAAGTTTTCCTTTTCACATCTTTATCAGCAATGTTCTTACCAGGGTCAGTGAAATCAAATGTCACACAACAAAGACTCAATTTGCTCACATACAGGTTCATCTTTTCAGCATTCGGAACATCCTTAAACGGCACCAAAGGTTCAATCCCAGAAACCATGCTAGCCGGAAACACTGCCGATGAAGAAGCTCGTTTCAACGAAGAAGCGCCACCTTGCTGCCGATTGTGGCATTTACCGGCAACACGTGGAGAATCTCCAACCTGGGTCGACTCATCTTCATCGATTTTGGAAGATTTCCGGGGAAGCTTGTTGAAAAGTTGCTTCAGCATTGCAACCCAGAAGCTGAAATCCACCAATTCCAAAGCTTTTACATCAAACCCATGGAGAAACAGTCAAAATCAACCAAACCCCAAAAAATTACTAATTCTGAACCAGAAAAACCCTAGATCTCGTTCACCTAAACAAATACAAATTTCAAGCAATCTAGATTTCCACCTGAAATTGAGATCCACATTTGAAATGGGGTTCAAGAAATTCTCAATCTTAAAGCATGCATTGATGTAAAAATCAAATCTTGAAGATTGCGGTTCGTGTGTTACGAAGAACACGTGAACCAAAAGAGGAAAGGTAAAACCAAAGGACGACAACCACAACAACAACAAAAAGTTTGTTACAAATAAACAGAACAACAACAATAAACAACAACAAACAATGATTACAATGATAATGTTACATCCGAATCAAATCTCACATTGGAACATTTTTTCCAATTTTTCTTAGGAATATGAAAATAAAAGAAAAATAAACTAANTTTTGGTATATTAATAAATGATGGATCTAGGAAGAAATGAAATGCATAGACTAAAGTTNTTTTTAGGTTAGGTTTTTTGGGGGGAGTAAGGTTAGGGTAATTAAGGTAAAAGTTAAAGGTAAAAGTTAAACGGGTAAAAAACCGGTTAACCCTTTACGTTTAA
TARGET=1627,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2226
PRIMER_LEFT_PENALTY=0.093130
PRIMER_RIGHT_PENALTY=1.129501
PRIMER_LEFT_SEQUENCE=AAGGTAAAACCAAAGGACGACA
PRIMER_RIGHT_SEQUENCE=AATTACCCTAACCTTACTCCCCC
PRIMER_LEFT=1601,22
PRIMER_RIGHT=1889,23
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=59.870
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=12.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=1.2226
PRIMER_LEFT_1_PENALTY=0.093130
PRIMER_RIGHT_1_PENALTY=1.129501
PRIMER_LEFT_1_SEQUENCE=AGGTAAAACCAAAGGACGACAA
PRIMER_RIGHT_1_SEQUENCE=AATTACCCTAACCTTACTCCCCC
PRIMER_LEFT_1=1602,22
PRIMER_RIGHT_1=1889,23
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=59.870
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=47.826
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=12.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=288
PRIMER_PAIR_PENALTY_2=1.3461
PRIMER_LEFT_2_PENALTY=0.216588
PRIMER_RIGHT_2_PENALTY=1.129501
PRIMER_LEFT_2_SEQUENCE=GTAAAACCAAAGGACGACAACC
PRIMER_RIGHT_2_SEQUENCE=AATTACCCTAACCTTACTCCCCC
PRIMER_LEFT_2=1604,22
PRIMER_RIGHT_2=1889,23
PRIMER_LEFT_2_TM=59.783
PRIMER_RIGHT_2_TM=59.870
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=47.826
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=12.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=1.3461
PRIMER_LEFT_3_PENALTY=0.216588
PRIMER_RIGHT_3_PENALTY=1.129501
PRIMER_LEFT_3_SEQUENCE=GGTAAAACCAAAGGACGACAAC
PRIMER_RIGHT_3_SEQUENCE=AATTACCCTAACCTTACTCCCCC
PRIMER_LEFT_3=1603,22
PRIMER_RIGHT_3=1889,23
PRIMER_LEFT_3_TM=59.783
PRIMER_RIGHT_3_TM=59.870
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.826
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=12.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=1.3834
PRIMER_LEFT_4_PENALTY=0.093130
PRIMER_RIGHT_4_PENALTY=1.290309
PRIMER_LEFT_4_SEQUENCE=AAGGTAAAACCAAAGGACGACA
PRIMER_RIGHT_4_SEQUENCE=ATTACCCTAACCTTACTCCCCC
PRIMER_LEFT_4=1601,22
PRIMER_RIGHT_4=1888,22
PRIMER_LEFT_4_TM=59.907
PRIMER_RIGHT_4_TM=58.710
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=12.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_05842 Ps_contig_mira-and-tgicl-ass_franssen_40177
SEQUENCE=TTCTGAGGGAAATACRTGCATTACCTCTCTATACATTGTATATATGACATGTTTGGAAATGTAAGAAAGGGAATTCTAAAACCTATATTTAACAGATGTTACAGTAGATAGATATAGATATAGATAAAACAATTTAAAGAATCAAAAGGTAGAAATGACTTCTCTATTTTTAGTGCTTTAGAATTTTACATCACTATTGTATATTAACAGAGAAGACATACCCACACAAATTATGGTAACTATATATAAAAAGGGCTTTATCCATTATTCATGCTTCTATTAAGAGAAATTCTGTAATCTTCACTGTTCTATAGGGTGCATGAGCAGTTCCTTAATCTTCATTGTTCTATAAGGCACATGAGCAGTTCTGTATTCTTCACTGTTCTATTGGGTGCATGAGCATGCACAAAATCTGAATCGTGCGTGCTCCTCAACACCATATTTATCTCTTCATAACAGTTTCCACTCAGGATAATCCTTACGCGGCGAAGCAAACCAAAAAATGGTACGTCCCAAAACAGAAAGAGATGAAACAATACCAGCAACATAAAACACCATAACCAACCCAATTATCCACGGCAGCAACATAAACCCAATCACGAAAGTAACCGAACCACACAACATCAGAGCCGTCGAAATTCCCAAAAGCAACGAAACGAATCCCGCCGGTGTTATCTTATTCGCCGCCATCGAACGCCTCAGCGACGACACCGACGGAGGCACCGGCATGACCGGAGCTCGATCGGAAAAAGTTAACGGCGTCGGCGGAAGCTGTAGAAGATTGAAAACCAGAGACGATAATTCACAGATAATCTTCGATTGTTGATCTTGATGTCCCCTCATCATGTTCTTCTTTTTTTTCTTCCGAAAATCAGAGGGAAAAAACGGAAAATATATAATCTAGAAATGAGAATCGATTTTCAAGCTTTTGTTTGTTTCACTTTTCTTGAAATTTGAAAAGGTTGACGACGACGTTTTGAGGGAAAAGACAAAGCGTTTGGTTGGTTGGGTTAAAGAAAAAGTGGGTTACATAAGGATTGGATAATTGGTTTGGAAAATGGTCCCCGACGTA
TARGET=998,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05843 Ps_contig_mira-and-tgicl-ass_franssen_40186
SEQUENCE=GGGGGATTTCACAAAAATCGNNGTCACCGTATCATGGCTTTCTTTCTTTGCGTCTTCGTTCTCTTTCTTGCTTCTTCTCTGCTACGGCTTGCGATCGTTGCGTACACCAATCCAAAGCTTCTTTTTTCTCCAAAGCTTCTACTCTTTCATCTGGAGCATGTGGCTATGGCTCTTTAGCACTTGACCTTAGTCATGGACAACTCGCAGCTGGCGCTTCTTCATTTTTCAGTAATGGAGCTGGTTGCGGTGCTTTGTTTTCAGGTAAGATGCAGGGACCAAGCTATATGCACAAAA
TARGET=38,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3386
PRIMER_LEFT_PENALTY=2.274479
PRIMER_RIGHT_PENALTY=0.064105
PRIMER_LEFT_SEQUENCE=GGGGATTTCACAAAAATCGN
PRIMER_RIGHT_SEQUENCE=CTGCGAGTTGTCCATGACTAAG
PRIMER_LEFT=1,20
PRIMER_RIGHT=206,22
PRIMER_LEFT_TM=60.274
PRIMER_RIGHT_TM=59.936
PRIMER_LEFT_GC_PERCENT=42.105
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.50
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.9750
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=2.3902
PRIMER_LEFT_1_PENALTY=2.274479
PRIMER_RIGHT_1_PENALTY=0.115769
PRIMER_LEFT_1_SEQUENCE=GGGGATTTCACAAAAATCGN
PRIMER_RIGHT_1_SEQUENCE=TATAGCTTGGTCCCTGCATCTT
PRIMER_LEFT_1=1,20
PRIMER_RIGHT_1=284,22
PRIMER_LEFT_1_TM=60.274
PRIMER_RIGHT_1_TM=60.116
PRIMER_LEFT_1_GC_PERCENT=42.105
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.50
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.9750
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=2.3902
PRIMER_LEFT_2_PENALTY=2.274479
PRIMER_RIGHT_2_PENALTY=0.115769
PRIMER_LEFT_2_SEQUENCE=GGGGATTTCACAAAAATCGN
PRIMER_RIGHT_2_SEQUENCE=ATAGCTTGGTCCCTGCATCTTA
PRIMER_LEFT_2=1,20
PRIMER_RIGHT_2=283,22
PRIMER_LEFT_2_TM=60.274
PRIMER_RIGHT_2_TM=60.116
PRIMER_LEFT_2_GC_PERCENT=42.105
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.50
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.9750
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_2=283
PRIMER_PAIR_PENALTY_3=2.6768
PRIMER_LEFT_3_PENALTY=2.274479
PRIMER_RIGHT_3_PENALTY=0.402305
PRIMER_LEFT_3_SEQUENCE=GGGGATTTCACAAAAATCGN
PRIMER_RIGHT_3_SEQUENCE=AAGTGCTAAAGAGCCATAGCCA
PRIMER_LEFT_3=1,20
PRIMER_RIGHT_3=181,22
PRIMER_LEFT_3_TM=60.274
PRIMER_RIGHT_3_TM=60.402
PRIMER_LEFT_3_GC_PERCENT=42.105
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.50
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.9750
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=2.6768
PRIMER_LEFT_4_PENALTY=2.274479
PRIMER_RIGHT_4_PENALTY=0.402305
PRIMER_LEFT_4_SEQUENCE=GGGGATTTCACAAAAATCGN
PRIMER_RIGHT_4_SEQUENCE=CAAGTGCTAAAGAGCCATAGCC
PRIMER_LEFT_4=1,20
PRIMER_RIGHT_4=182,22
PRIMER_LEFT_4_TM=60.274
PRIMER_RIGHT_4_TM=60.402
PRIMER_LEFT_4_GC_PERCENT=42.105
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.50
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.9750
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_05844 Ps_contig_mira-and-tgicl-ass_franssen_40188
SEQUENCE=GAACAAACGAAAAGAATTNGGTTATGCAAAAAAGCTTGTAAATGTGTCACGTGTACTTGCGAATCATATACATACCTTTTCAATGGTACATTACAGCATTGAAACTATCAGTATATAAAGATCAAAATTCTCCATGGCAGACTAGTTACTAAAGTGTAGTAATTATTTTACTCATCATCATCAGAATCACTGTATTCATAATCAGAATCCGATTCATCATTCCTTGGTCGCATCAATGAATTCATGTCAAACGATGGTTTCATTTCCATCTCTTCCATGTCAATAGTGTCATAATCCATCCGTTGCTCAAATTTCTCATTAGAACAATTAAGCATCCATGCCAGCGAACACTTCAATCCTTTCTTTGCGACCATGTTATGCCTAGGTTTTATAGTTGACTCCAAACCATAAGTGAAAAATGCTGGGAATTCAACCAAGTCTTCCAAAGGCCGTTCCATCTCGCTTTGCGAGTAATCAAAGCTAAGTTTCATGATATCAATGTTTAAAGCAAGTAGTTGAGGGCACCCAACAATCATCTTCTTCATTTGCAGCAGGGAAAATCCACATTCCTTAAGAAAATCAACATGCTTTAGCATAGGTGTACTACTGAGGCTTACTACCTGCGGCATTTTCTCGACGATTAAGCCGAAATCCTCAGGATCCAAATCAAGTACTGAGTTGAGAACGGTTTTCTTATTGGCAAGCTTTGGCTCTAGATCGGCTCCAATGATGTCAGGATACTGAGCGATTATCGAGGGAATTGATGTTCGTCGAACATTAAACTCTTCGAGGGATTTGACGTTTGGCTTCACCTTCTCGTCGAGATCGAATCCAAGAATATAAGGTTGTGTCTCTATCAGTCTAGCAATGGCTAGCCTTGGAACACCCAAGCTTTCTAGATACTCCACAAAAGGCTTGATGACTCTACCAACCCGCATCCCCAAAATCTCCGGGTATCTAGTTAAGATGCCTCCGAGTTCTCTTCTTCCAACACCAATTCCGATCAAATAAGCAACTGACGTGCTCATAGTTCCCTCAAGTTTGAATCCTAGCACCTCCGGATACCTCTCAAGAACACGAGGAATATCATTGGGTTTGATATCCATCCCTTGAAGATATTTAACCACCGGCACAAGATCCACAACCACACTAGCATGAAGCACTTGCGGGTATCTCTGCAAAAACTTCGTCAACGTGGATTTCCTAACTCCCAATTTACCAAGGTAATCAAGCACAGGCACCATGTTCTTCTTGACGCTGCATCCAAGAACAAGAGGATAGTTGTTAATGTCTTCAATCGTAAGCCCCAAGTTATGAAGAAAATCCACACGCTCCTTCATAACATCAAGCGTAACAGGAAGTTCAAGACCATCAAGCTCATCAGGAACAATGCCAATTCCTCGCAAGAACTCATAAACATTTTCACGCAACGCCGCCTTATCTTTCTTCATTTCATATAAACTAGGACGAATGTAACCAACATATGGACCTCCCCGTTTCCTTTGAATTTCACCAGAGTCCCGATTAGGTCCCCGTGAACCACCGGCACTGGAACTATACGTCATATCAGCAACAGAACACTGAAGCTTTGTAATCAACCTCATCTTCCTAACACAAATACATGGCAAATGAAAAGCTAGGGTAGCAGTCAACTTAGAATGACCAAATGTCAAAATTGGCATTTCTGTTTGAGAAAACGGAAAATTGGGTTTTGTTATGGCACAAGAAAAGTTCATAACTTTCATATTTTTCAAGAGTTGAGTACCATTGAATAGTGAGGGGGAAATGATGACGGTTTGTTGAATTGAAGTTAGAATCTTTTGGCTCTTCCTCGAACAGTGATAAGGGATAACAGAAACTGGAAGAGGGTTTTACCTGCGATGTTTGTGTTTGAAAGGATTNATTTATGTGTATTTCGTTTGGGAAT
TARGET=172,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1339
PRIMER_LEFT_PENALTY=0.066501
PRIMER_RIGHT_PENALTY=0.067436
PRIMER_LEFT_SEQUENCE=GTCACGTGTACTTGCGAATCAT
PRIMER_RIGHT_SEQUENCE=TGACATGGAAGAGATGGAAATG
PRIMER_LEFT=45,22
PRIMER_RIGHT=281,22
PRIMER_LEFT_TM=60.067
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.3656
PRIMER_LEFT_1_PENALTY=0.066501
PRIMER_RIGHT_1_PENALTY=0.299089
PRIMER_LEFT_1_SEQUENCE=GTCACGTGTACTTGCGAATCAT
PRIMER_RIGHT_1_SEQUENCE=TGGAAGAGATGGAAATGAAACC
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=276,22
PRIMER_LEFT_1_TM=60.067
PRIMER_RIGHT_1_TM=60.299
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.3656
PRIMER_LEFT_2_PENALTY=0.066501
PRIMER_RIGHT_2_PENALTY=0.299089
PRIMER_LEFT_2_SEQUENCE=GTCACGTGTACTTGCGAATCAT
PRIMER_RIGHT_2_SEQUENCE=GGAAGAGATGGAAATGAAACCA
PRIMER_LEFT_2=45,22
PRIMER_RIGHT_2=275,22
PRIMER_LEFT_2_TM=60.067
PRIMER_RIGHT_2_TM=60.299
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.3861
PRIMER_LEFT_3_PENALTY=0.066501
PRIMER_RIGHT_3_PENALTY=0.319597
PRIMER_LEFT_3_SEQUENCE=GTCACGTGTACTTGCGAATCAT
PRIMER_RIGHT_3_SEQUENCE=AGAGATGGAAATGAAACCATCG
PRIMER_LEFT_3=45,22
PRIMER_RIGHT_3=272,22
PRIMER_LEFT_3_TM=60.067
PRIMER_RIGHT_3_TM=60.320
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.5000
PRIMER_RIGHT_3_END_STABILITY=8.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=0.4061
PRIMER_LEFT_4_PENALTY=0.066501
PRIMER_RIGHT_4_PENALTY=0.339582
PRIMER_LEFT_4_SEQUENCE=GTCACGTGTACTTGCGAATCAT
PRIMER_RIGHT_4_SEQUENCE=GGCATGGATGCTTAATTGTTCT
PRIMER_LEFT_4=45,22
PRIMER_RIGHT_4=341,22
PRIMER_LEFT_4_TM=60.067
PRIMER_RIGHT_4_TM=60.340
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.5000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_05845 Ps_contig_mira-and-tgicl-ass_franssen_40200
SEQUENCE=GTCTCTCTGAACCATTTCCAATTCGATTGAAAATTTTGACTCTCTCTCTGTTTCTCAATTTCGCTCTTTCTCTGCTTTTTCTCTCTCATCAAAACGACGTAGCTTTTCCTCTGCGCCACTTTCTCCTCCGGTAACCGGGGGAGTCGCACCGCCGGCGTTCCCCTTCCCTGATTTCCTCACCAACCTTCAATTCAACCTTTCCCGTTTCCTTTCACTGATTTCTTCTCACAAAATGGCTCACACCACTTGCTAGGGTTTCACCCCTCTCAAATCTTTCTCTCCAACACCACCCTGTACCTTTTCTTCCTCAACCTAATTCACTTCATCTTCATGGCTATACGTGTAACCTTCTCCTTTTCAGGCTATGTCGCCCAAAATCTAGTTTCCTCCGCCGGCGTTCGAGTCGCCAATTCTCGTTGTGTCCAAGAATGCTGCATCCTCTCTCGCTTCTTTGGAACCAACCAGAAACGCGATCTCGAACGTTCCGGCGGCGTTCGCAGTCTCTACCCCGATCTCCGGAGACCTAAAAATTCATCTCCTGTTTCCGCCTATTCCACCCTCGCCGGAGAGATTCTCACCGAAGGATGTACGAATCCGATCGTATTAGGTTTGATTTCGATGATGAAGTCAACGAGTGTTGTATCCGGGTCAACTTCGGCGGCTATGGGTATTATGGGTATTGCACCATTCAAAACCTCTTCGATTATACCATTTTTGCAGGGTTCCAAGTGGCTTCCTTGCAACGAATCTGTTCCTGAACCTACAACGTGGGAGGTTGATAAAGGTGGAACGGAGTGCGTTACTGTTTCTAACACAAAATCGCGTTTGAGTCAAAAGGAGACTAGTGGTTGGATTTCAAAATTGTTGAGTGTTTGTTCAGAAGATGCTAAAGCTGTGTTCACTGCTGTTACTGTTAGTTTACTGTTTAAATCGTTCTTGGCTGAACCTAAATCTATTCCTTCTGCTTCTATGTATCCAACTCTTGAAGTTGGTGATCGTGTTTTAACAGAAAAGTTTTCATTCTTCTTTAGAAAACCAGATGTTTCTGATATTGTGATTTTCAAAGCACCTCCTTGTTTGAAGAAATTTGGTTTTAGTTCATCTGATATATTTATAAAAAGGGTTGTGGCTAAGGCTGGTGATGTTGTGGAAGTTCGTGGTGGGAAACTACTAGTGAATGGTGTCGCTGAAGAAGAGGAATTTGTATTAGAACCTCTTGCTTATGAGTTGGCTTCAATAGTTGTGCCAGAAGGACATGTGTTTGTGATGGGGGACAATCGTAACAAGAGTTTTGATTCTCATAACTGGGGTCCACTCCCAATCGAGAACATTGTTGGTAGGTCCATGTTTCGCTACTGGCCTCCATCCAAAGCTGCGGACACTGTCTCCGTTCATAACTCCCCACCTGGAAATAAATCTGTGGCAGTTTCTTAACATGATTTCCACTACATTCTGAGGAGATAAAAGATTATTGATACATTCATTTATTCTGAATGAATTGCTACTCCGGTTTTGGACAGGGAGTGAGCTGTTACTCAACAATTCTCCAGGACGAGTCCGCATGTCTTGATTATTGTTTGAGATGAATATCTTATTCATTGATGCAATTTTGTCAATGACCTCATAATACTGTTCTTTAGTGCTATCCTGAGATGTATGGCCCCTTGCAAAAGTTGTGGGGGAATAGAAAAATTTAAGAAACATGATGGTGGGTTTTTGTTGTATTCTTTCATGTGTTTTTTAAAATCTGAATGCTAGAAACTTGAGGGAAAGCTTCAACATGGAGCTATTTGACTCCTTGTAAGTAAGTTTCATAATAAAATTGCCTCATTGTTTGTAATTGATATGAAAGAAATTATCTGAGCATGTTTTTTTTCTTACG
TARGET=40,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.4342
PRIMER_LEFT_PENALTY=2.214320
PRIMER_RIGHT_PENALTY=0.219884
PRIMER_LEFT_SEQUENCE=CTCTCTGAACCATTTCCAATTC
PRIMER_RIGHT_SEQUENCE=GTGTGAGCCATTTTGTGAGAAG
PRIMER_LEFT=2,22
PRIMER_RIGHT=242,22
PRIMER_LEFT_TM=57.786
PRIMER_RIGHT_TM=59.780
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=241
PRIMER_PAIR_PENALTY_1=2.5424
PRIMER_LEFT_1_PENALTY=2.214320
PRIMER_RIGHT_1_PENALTY=0.328094
PRIMER_LEFT_1_SEQUENCE=CTCTCTGAACCATTTCCAATTC
PRIMER_RIGHT_1_SEQUENCE=GGAAAAGCTACGTCGTTTTGAT
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=108,22
PRIMER_LEFT_1_TM=57.786
PRIMER_RIGHT_1_TM=59.672
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=107
PRIMER_PAIR_PENALTY_2=2.5682
PRIMER_LEFT_2_PENALTY=2.348289
PRIMER_RIGHT_2_PENALTY=0.219884
PRIMER_LEFT_2_SEQUENCE=GTCTCTCTGAACCATTTCCAATTC
PRIMER_RIGHT_2_SEQUENCE=GTGTGAGCCATTTTGTGAGAAG
PRIMER_LEFT_2=0,24
PRIMER_RIGHT_2=242,22
PRIMER_LEFT_2_TM=60.348
PRIMER_RIGHT_2_TM=59.780
PRIMER_LEFT_2_GC_PERCENT=41.667
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=243
PRIMER_PAIR_PENALTY_3=2.6764
PRIMER_LEFT_3_PENALTY=2.348289
PRIMER_RIGHT_3_PENALTY=0.328094
PRIMER_LEFT_3_SEQUENCE=GTCTCTCTGAACCATTTCCAATTC
PRIMER_RIGHT_3_SEQUENCE=GGAAAAGCTACGTCGTTTTGAT
PRIMER_LEFT_3=0,24
PRIMER_RIGHT_3=108,22
PRIMER_LEFT_3_TM=60.348
PRIMER_RIGHT_3_TM=59.672
PRIMER_LEFT_3_GC_PERCENT=41.667
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=2.6926
PRIMER_LEFT_4_PENALTY=2.214320
PRIMER_RIGHT_4_PENALTY=0.478252
PRIMER_LEFT_4_SEQUENCE=CTCTCTGAACCATTTCCAATTC
PRIMER_RIGHT_4_SEQUENCE=GCTACGTCGTTTTGATGAGAGA
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=102,22
PRIMER_LEFT_4_TM=57.786
PRIMER_RIGHT_4_TM=59.522
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=101
=
PRIMER_SEQUENCE_ID=SSR_05846 Ps_contig_mira-and-tgicl-ass_franssen_40207
SEQUENCE=CGCTTTGTGTGCGTTTCTCTCTCTGGTAATGTTAAAAGTTTGGAGAAAGAGAGAAGAATAGAGAGAAGAGAAGAGAAAAGGAAGAAGAAGAAGAGGCAACCCTAATTCCATTTCTTCAAGAGATGTTGCCTTTACATGGGTAACGTGCAGGTTCAGAGGATAGTGGTGCTCCGCACGAGGCTAGTGAGGAGTAGTCTTAGATCTATTTCCTATCCTTAGGTAGTTGATCATTTGCTCTAATCATGTAACACGGGGTAGCTGGATATTATGTCTGACTTGATATATTATACTTTATTTTTTCTTGTCTGGATTTATGT
TARGET=62,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0808
PRIMER_LEFT_PENALTY=0.052187
PRIMER_RIGHT_PENALTY=0.028588
PRIMER_LEFT_SEQUENCE=TGTGCGTTTCTCTCTCTGGTAA
PRIMER_RIGHT_SEQUENCE=GGAATTAGGGTTGCCTCTTCTT
PRIMER_LEFT=7,22
PRIMER_RIGHT=108,22
PRIMER_LEFT_TM=60.052
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=0.1913
PRIMER_LEFT_1_PENALTY=0.052187
PRIMER_RIGHT_1_PENALTY=0.139083
PRIMER_LEFT_1_SEQUENCE=TGTGCGTTTCTCTCTCTGGTAA
PRIMER_RIGHT_1_SEQUENCE=GAGCACCACTATCCTCTGAACC
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=170,22
PRIMER_LEFT_1_TM=60.052
PRIMER_RIGHT_1_TM=60.139
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.1913
PRIMER_LEFT_2_PENALTY=0.052187
PRIMER_RIGHT_2_PENALTY=0.139083
PRIMER_LEFT_2_SEQUENCE=TGTGCGTTTCTCTCTCTGGTAA
PRIMER_RIGHT_2_SEQUENCE=GGAGCACCACTATCCTCTGAAC
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=171,22
PRIMER_LEFT_2_TM=60.052
PRIMER_RIGHT_2_TM=60.139
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.2513
PRIMER_LEFT_3_PENALTY=0.052187
PRIMER_RIGHT_3_PENALTY=0.199067
PRIMER_LEFT_3_SEQUENCE=TGTGCGTTTCTCTCTCTGGTAA
PRIMER_RIGHT_3_SEQUENCE=AATGGAATTAGGGTTGCCTCTT
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=111,22
PRIMER_LEFT_3_TM=60.052
PRIMER_RIGHT_3_TM=60.199
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=105
PRIMER_PAIR_PENALTY_4=0.2513
PRIMER_LEFT_4_PENALTY=0.052187
PRIMER_RIGHT_4_PENALTY=0.199067
PRIMER_LEFT_4_SEQUENCE=TGTGCGTTTCTCTCTCTGGTAA
PRIMER_RIGHT_4_SEQUENCE=AAATGGAATTAGGGTTGCCTCT
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=112,22
PRIMER_LEFT_4_TM=60.052
PRIMER_RIGHT_4_TM=60.199
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=106
=
PRIMER_SEQUENCE_ID=SSR_05847 Ps_contig_mira-and-tgicl-ass_franssen_40207
SEQUENCE=CGCTTTGTGTGCGTTTCTCTCTCTGGTAATGTTAAAAGTTTGGAGAAAGAGAGAAGAATAGAGAGAAGAGAAGAGAAAAGGAAGAAGAAGAAGAGGCAACCCTAATTCCATTTCTTCAAGAGATGTTGCCTTTACATGGGTAACGTGCAGGTTCAGAGGATAGTGGTGCTCCGCACGAGGCTAGTGAGGAGTAGTCTTAGATCTATTTCCTATCCTTAGGTAGTTGATCATTTGCTCTAATCATGTAACACGGGGTAGCTGGATATTATGTCTGACTTGATATATTATACTTTATTTTTTCTTGTCTGGATTTATGT
TARGET=81,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1913
PRIMER_LEFT_PENALTY=0.052187
PRIMER_RIGHT_PENALTY=0.139083
PRIMER_LEFT_SEQUENCE=TGTGCGTTTCTCTCTCTGGTAA
PRIMER_RIGHT_SEQUENCE=GAGCACCACTATCCTCTGAACC
PRIMER_LEFT=7,22
PRIMER_RIGHT=170,22
PRIMER_LEFT_TM=60.052
PRIMER_RIGHT_TM=60.139
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=164
PRIMER_PAIR_PENALTY_1=0.1913
PRIMER_LEFT_1_PENALTY=0.052187
PRIMER_RIGHT_1_PENALTY=0.139083
PRIMER_LEFT_1_SEQUENCE=TGTGCGTTTCTCTCTCTGGTAA
PRIMER_RIGHT_1_SEQUENCE=GGAGCACCACTATCCTCTGAAC
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=171,22
PRIMER_LEFT_1_TM=60.052
PRIMER_RIGHT_1_TM=60.139
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=165
PRIMER_PAIR_PENALTY_2=0.2513
PRIMER_LEFT_2_PENALTY=0.052187
PRIMER_RIGHT_2_PENALTY=0.199067
PRIMER_LEFT_2_SEQUENCE=TGTGCGTTTCTCTCTCTGGTAA
PRIMER_RIGHT_2_SEQUENCE=AGAAATGGAATTAGGGTTGCCT
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=114,22
PRIMER_LEFT_2_TM=60.052
PRIMER_RIGHT_2_TM=60.199
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=108
PRIMER_PAIR_PENALTY_3=0.2631
PRIMER_LEFT_3_PENALTY=0.052187
PRIMER_RIGHT_3_PENALTY=0.210886
PRIMER_LEFT_3_SEQUENCE=TGTGCGTTTCTCTCTCTGGTAA
PRIMER_RIGHT_3_SEQUENCE=AGCTACCCCGTGTTACATGATT
PRIMER_LEFT_3=7,22
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=60.052
PRIMER_RIGHT_3_TM=59.789
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=253
PRIMER_PAIR_PENALTY_4=0.2945
PRIMER_LEFT_4_PENALTY=0.052187
PRIMER_RIGHT_4_PENALTY=0.242357
PRIMER_LEFT_4_SEQUENCE=TGTGCGTTTCTCTCTCTGGTAA
PRIMER_RIGHT_4_SEQUENCE=TAATATCCAGCTACCCCGTGTT
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=267,22
PRIMER_LEFT_4_TM=60.052
PRIMER_RIGHT_4_TM=59.758
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=261
=
PRIMER_SEQUENCE_ID=SSR_05848 Ps_contig_mira-and-tgicl-ass_franssen_40208
SEQUENCE=ATTGCAATAGAAAGGTTTAGTTTTGGGTTTTGATACCGCGGGAAGGTGGGCTTATGTAAGAACGCGAGCGACGTTGTCCCGCAGGCAGCCACACATCCATCTCTCCTCAGTCGCAGATCACGTCTCCTCTCCTCTCCTCTCCTCCGCCGCGCCGCCTTCTTCCTCACCTCGTCCACCGTTCTTCACCTAAGAACCAAGAATTCAGAACTGCACCACTTTTTCTACTTCCCCAAGTGATTCTTACACTACTCTGATTACTCCTAAAAATGTTTCGAACATGGACCAAAGCAATTATTCCACTCTCCAAACTTCGAGCTCCAATGCATCAAACCTACGCTAGGGTTGCAGCTGCACCAATCATAGAAGACAAAGTTGAACCTATTGTTAACCTAGACAAAATGTTTTGGGCTAAACCCTGTTCTTTGGCACTGCCCCGTGACTCGCCTCTAAGAATTGATGAACCTGATTATCAGGGGGTTAAGCGTTTCATGCTCAAACTCATGCTGTTTTATAGTAAGCAGAGCAAATCAATTCGAGGGGCTAATGTTGTTTACAAGAGAATTGTCTCTCAAGTTGATAAACCACTTATTTATGAAGTGTTTAACTTGGAGAAAACATTCAAAACAACGTTCTCTTTGCTCGTACTTCACATGTGGTTTTACTTGCGCCGCATTAAGCAAGAGGGAAGTGAGGGTGTTGAATTTGGCCAATACCTTTATGAAATTTACAATCACGATGTGGAGCTTAGAGTCTCCAAAGCTGGGGTTAACCTACTATTGATTAAGTGGATGAAGGAGCTTGAGAAGATTTTTTATGGGAACATTGTTGCTTATGATGCTGCTATACTGCCAGAAGCTAAACCGGGTGATTTCGCTAGTATTATATGGAGGAATATATTCTCTGATGATGGTTCTTCAACACCAGACAAAGCTGCATTGCAATCTGTGCAGGCATTGGCCAGGTATGCTCAAAGGGAAGTAAGTTGCATGACTTTAACAGATAAAGAAGCACTATTATCGGGGAATTTCACGTTTACCTCTTTGAAGCATGAGATCAAAAGCAAAAATGGGCACCAATTATAAAGTAATGGTTTCTAAATTTACAATTAAGTAAATGTAAAATACATGCTGTTGTCATTTTTTTCTCAATAATCACAATATAAGATTCTATTGTTCTTCNTAAAGCTTTATTATTTAGTTAGTTCTGCT
TARGET=123,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2653
PRIMER_LEFT_PENALTY=0.232153
PRIMER_RIGHT_PENALTY=0.033137
PRIMER_LEFT_SEQUENCE=TAGTTTTGGGTTTTGATACCGC
PRIMER_RIGHT_SEQUENCE=GTTCTTAGGTGAAGAACGGTGG
PRIMER_LEFT=17,22
PRIMER_RIGHT=193,22
PRIMER_LEFT_TM=60.232
PRIMER_RIGHT_TM=60.033
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=11.1000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.2653
PRIMER_LEFT_1_PENALTY=0.232153
PRIMER_RIGHT_1_PENALTY=0.033137
PRIMER_LEFT_1_SEQUENCE=TAGTTTTGGGTTTTGATACCGC
PRIMER_RIGHT_1_SEQUENCE=GGTTCTTAGGTGAAGAACGGTG
PRIMER_LEFT_1=17,22
PRIMER_RIGHT_1=194,22
PRIMER_LEFT_1_TM=60.232
PRIMER_RIGHT_1_TM=60.033
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=11.1000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=178
PRIMER_PAIR_PENALTY_2=0.2653
PRIMER_LEFT_2_PENALTY=0.232153
PRIMER_RIGHT_2_PENALTY=0.033137
PRIMER_LEFT_2_SEQUENCE=TAGTTTTGGGTTTTGATACCGC
PRIMER_RIGHT_2_SEQUENCE=TGGTTCTTAGGTGAAGAACGGT
PRIMER_LEFT_2=17,22
PRIMER_RIGHT_2=195,22
PRIMER_LEFT_2_TM=60.232
PRIMER_RIGHT_2_TM=60.033
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=9.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.4520
PRIMER_LEFT_3_PENALTY=0.232153
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=TAGTTTTGGGTTTTGATACCGC
PRIMER_RIGHT_3_SEQUENCE=GTGGTGCAGTTCTGAATTCTTG
PRIMER_LEFT_3=17,22
PRIMER_RIGHT_3=215,22
PRIMER_LEFT_3_TM=60.232
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=199
PRIMER_PAIR_PENALTY_4=0.4726
PRIMER_LEFT_4_PENALTY=0.439481
PRIMER_RIGHT_4_PENALTY=0.033137
PRIMER_LEFT_4_SEQUENCE=AAGGTGGGCTTATGTAAGAACG
PRIMER_RIGHT_4_SEQUENCE=GTTCTTAGGTGAAGAACGGTGG
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=193,22
PRIMER_LEFT_4_TM=59.561
PRIMER_RIGHT_4_TM=60.033
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_05849 Ps_contig_mira-and-tgicl-ass_franssen_40209
SEQUENCE=GGGAAGAATNATGAATCAACAANAATTTGATAAAANAAGGAGTGAAGATGGGAAGTAATGATTNAAGACAGAANAGTTTCTAATAAAANAAGGTTACATTTGCAGAACTTATAGATTTGACAGTTGCAGAATGCAGAGAAAAGCGTGTTAGAGTAGTTAGTATGAGAATAATGTAAAACTGAGTGATGTATACAAATTCAATTCAATTCAAATGGAGTCTAACTGCAGTAATTTTTTGTGATTAGCATATGATCTTTTCCTTTGAGTGCTTAGATAATTTATGTGGAAGCAAGTAGGGGTAGGGTTGTACTTTCCC
TARGET=195,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1671
PRIMER_LEFT_PENALTY=0.059316
PRIMER_RIGHT_PENALTY=0.107763
PRIMER_LEFT_SEQUENCE=ACAGTTGCAGAATGCAGAGAAA
PRIMER_RIGHT_SEQUENCE=ACCCCTACTTGCTTCCACATAA
PRIMER_LEFT=119,22
PRIMER_RIGHT=299,22
PRIMER_LEFT_TM=60.059
PRIMER_RIGHT_TM=59.892
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.2793
PRIMER_LEFT_1_PENALTY=0.171564
PRIMER_RIGHT_1_PENALTY=0.107763
PRIMER_LEFT_1_SEQUENCE=TGCAGAGAAAAGCGTGTTAGAG
PRIMER_RIGHT_1_SEQUENCE=ACCCCTACTTGCTTCCACATAA
PRIMER_LEFT_1=131,22
PRIMER_RIGHT_1=299,22
PRIMER_LEFT_1_TM=59.828
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.3453
PRIMER_LEFT_2_PENALTY=0.059316
PRIMER_RIGHT_2_PENALTY=0.285981
PRIMER_LEFT_2_SEQUENCE=ACAGTTGCAGAATGCAGAGAAA
PRIMER_RIGHT_2_SEQUENCE=ACAACCCTACCCCTACTTGCTT
PRIMER_LEFT_2=119,22
PRIMER_RIGHT_2=307,22
PRIMER_LEFT_2_TM=60.059
PRIMER_RIGHT_2_TM=60.286
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.4152
PRIMER_LEFT_3_PENALTY=0.059316
PRIMER_RIGHT_3_PENALTY=0.355872
PRIMER_LEFT_3_SEQUENCE=ACAGTTGCAGAATGCAGAGAAA
PRIMER_RIGHT_3_SEQUENCE=CCCCTACTTGCTTCCACATAAA
PRIMER_LEFT_3=119,22
PRIMER_RIGHT_3=298,22
PRIMER_LEFT_3_TM=60.059
PRIMER_RIGHT_3_TM=60.356
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.4207
PRIMER_LEFT_4_PENALTY=0.312895
PRIMER_RIGHT_4_PENALTY=0.107763
PRIMER_LEFT_4_SEQUENCE=ATTTGACAGTTGCAGAATGCAG
PRIMER_RIGHT_4_SEQUENCE=ACCCCTACTTGCTTCCACATAA
PRIMER_LEFT_4=114,22
PRIMER_RIGHT_4=299,22
PRIMER_LEFT_4_TM=60.313
PRIMER_RIGHT_4_TM=59.892
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_05850 Ps_contig_mira-and-tgicl-ass_franssen_40212
SEQUENCE=AGGTTTTGGGTTTGAGTTTGACCCAAATTAAGTTCACTTTTATTTTATTTTATTTTTTTCTTTGTTGTACAGGTACGGTTGGAATATTATGAAGCATAAAATTTTATAAAGTTAACATAGAAAGTAAAAAAAGGAAGTGTTGTAAGAGAAAGGGAACAGCTTACAACAGAACAATATACGTGTATGTATTACAAATTAAAGTTTGTTGATTCATTTCTTCTTTTCATTGGTATATCATGTTTAAAACAAAATTAAAAGTAATATATGGTAAAAGAGACATTGGTCAATTCGATAATTTAGTTTGGAAGTGGAAATGTTCATCGGAATAATGTGTTGGATATCAATATGGTTGTGTGGGAATGAGACGAAAATTAAGTAGAAATAGTAAATATTAGTTGTATCTATTCAAAATAGATTTTGGAGGTTATTTTTACCC
TARGET=38,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3035
PRIMER_LEFT_PENALTY=0.589035
PRIMER_RIGHT_PENALTY=0.714453
PRIMER_LEFT_SEQUENCE=AGGTTTTGGGTTTGAGTTTGAC
PRIMER_RIGHT_SEQUENCE=TGTTCTGTTGTAAGCTGTTCCC
PRIMER_LEFT=0,22
PRIMER_RIGHT=172,22
PRIMER_LEFT_TM=59.411
PRIMER_RIGHT_TM=59.286
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=1.7770
PRIMER_LEFT_1_PENALTY=0.589035
PRIMER_RIGHT_1_PENALTY=1.187985
PRIMER_LEFT_1_SEQUENCE=AGGTTTTGGGTTTGAGTTTGAC
PRIMER_RIGHT_1_SEQUENCE=TGTTGTAAGCTGTTCCCTTTCTC
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=167,23
PRIMER_LEFT_1_TM=59.411
PRIMER_RIGHT_1_TM=59.812
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=168
PRIMER_PAIR_PENALTY_2=1.7770
PRIMER_LEFT_2_PENALTY=0.589035
PRIMER_RIGHT_2_PENALTY=1.187985
PRIMER_LEFT_2_SEQUENCE=AGGTTTTGGGTTTGAGTTTGAC
PRIMER_RIGHT_2_SEQUENCE=TCTGTTGTAAGCTGTTCCCTTTC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=169,23
PRIMER_LEFT_2_TM=59.411
PRIMER_RIGHT_2_TM=59.812
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=1.7770
PRIMER_LEFT_3_PENALTY=0.589035
PRIMER_RIGHT_3_PENALTY=1.187985
PRIMER_LEFT_3_SEQUENCE=AGGTTTTGGGTTTGAGTTTGAC
PRIMER_RIGHT_3_SEQUENCE=GCTGTTCCCTTTCTCTTACAACA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=159,23
PRIMER_LEFT_3_TM=59.411
PRIMER_RIGHT_3_TM=59.812
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=160
PRIMER_PAIR_PENALTY_4=1.8010
PRIMER_LEFT_4_PENALTY=0.589035
PRIMER_RIGHT_4_PENALTY=1.211969
PRIMER_LEFT_4_SEQUENCE=AGGTTTTGGGTTTGAGTTTGAC
PRIMER_RIGHT_4_SEQUENCE=TGTTCTGTTGTAAGCTGTTCCCT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=172,23
PRIMER_LEFT_4_TM=59.411
PRIMER_RIGHT_4_TM=60.212
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_05851 Ps_contig_mira-and-tgicl-ass_franssen_40218
SEQUENCE=GATTGTTATCGTCAAAATTCACCAAACAGATTTCTAAAGTAATTTCATTCTCAGCTAATTAGTATGACTTGCCTGGTTACCCATAGAAGACATCATATTTCACAGACTGATATTGAGTTAAAAAAGGGCCATTAATTTTTAAATATTTAAAGAAGCTAAGAATAAAATCTTAAACTAAACTCTGTTCAACTCAGTATTATGTTTGTCCAACTGATCTGTCAACTCTTTCTTCCACTCCTGGATCAAATGTTCTTGTTTTTTAATTCAGCTCGTTTTTTTCGTTTAAGCTCATCTTCCATAAGAGCAATCTCCTTTTTGAGCATTTCAGCTTTTGATGGCTTATCCTCTCGCTGCAGACTAATAAAATAAAGTTGAAGTTTCTTGGCGGCCTCCATAAAATCTCTAGCATGGCGATCCACGTCGATTTGATGAGAAGGATGGGGGGAACGGTCTATGGCTTGAAGCTCTTTGGCTGGCAAACAGGGAAGCAGAGCAGCCTCCAAAGCCATAACACATGAAATCATATCATCCTTTGAGGGAGGAGGAGGAGGTGGAAGAGGAGGAGAAGATGGCAGCTGTTGATCAACTGTGTCAACTACTTGTCGGTCACCCATATTTTCAGACCGCTGCGCAGCAGACGGTGAGTGGGAAGAGGTGAACGACGACTGGTTCTGAGGCGGCGCAAGGTGAAGAACGGGATTTCTGTTGGCGTTCTCTTCTCTTCTCAGATGAATCAGGGCGTGGGAATGTATGTGAGATTCTCCGTTTTCTTCCCTCAGTTAGATACGAGAATAAGAGGAAACGAATTCCACA
TARGET=538,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2469
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.243298
PRIMER_LEFT_SEQUENCE=CTCCAAAGCCATAACACATGAA
PRIMER_RIGHT_SEQUENCE=CTAACTGAGGGAAGAAAACGGA
PRIMER_LEFT=497,22
PRIMER_RIGHT=782,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.757
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.2469
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.243298
PRIMER_LEFT_1_SEQUENCE=CTCCAAAGCCATAACACATGAA
PRIMER_RIGHT_1_SEQUENCE=TAACTGAGGGAAGAAAACGGAG
PRIMER_LEFT_1=497,22
PRIMER_RIGHT_1=781,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=59.757
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.2469
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.243298
PRIMER_LEFT_2_SEQUENCE=CTCCAAAGCCATAACACATGAA
PRIMER_RIGHT_2_SEQUENCE=TCTAACTGAGGGAAGAAAACGG
PRIMER_LEFT_2=497,22
PRIMER_RIGHT_2=783,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.2527
PRIMER_LEFT_3_PENALTY=0.003589
PRIMER_RIGHT_3_PENALTY=0.249074
PRIMER_LEFT_3_SEQUENCE=CTCCAAAGCCATAACACATGAA
PRIMER_RIGHT_3_SEQUENCE=GAAGAGAACGCCAACAGAAATC
PRIMER_LEFT_3=497,22
PRIMER_RIGHT_3=718,22
PRIMER_LEFT_3_TM=59.996
PRIMER_RIGHT_3_TM=60.249
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.2715
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=0.267873
PRIMER_LEFT_4_SEQUENCE=CTCCAAAGCCATAACACATGAA
PRIMER_RIGHT_4_SEQUENCE=TCTGAGAAGAGAAGAGAACGCC
PRIMER_LEFT_4=497,22
PRIMER_RIGHT_4=728,22
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=60.268
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=11.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_05852 Ps_contig_mira-and-tgicl-ass_franssen_40218
SEQUENCE=GATTGTTATCGTCAAAATTCACCAAACAGATTTCTAAAGTAATTTCATTCTCAGCTAATTAGTATGACTTGCCTGGTTACCCATAGAAGACATCATATTTCACAGACTGATATTGAGTTAAAAAAGGGCCATTAATTTTTAAATATTTAAAGAAGCTAAGAATAAAATCTTAAACTAAACTCTGTTCAACTCAGTATTATGTTTGTCCAACTGATCTGTCAACTCTTTCTTCCACTCCTGGATCAAATGTTCTTGTTTTTTAATTCAGCTCGTTTTTTTCGTTTAAGCTCATCTTCCATAAGAGCAATCTCCTTTTTGAGCATTTCAGCTTTTGATGGCTTATCCTCTCGCTGCAGACTAATAAAATAAAGTTGAAGTTTCTTGGCGGCCTCCATAAAATCTCTAGCATGGCGATCCACGTCGATTTGATGAGAAGGATGGGGGGAACGGTCTATGGCTTGAAGCTCTTTGGCTGGCAAACAGGGAAGCAGAGCAGCCTCCAAAGCCATAACACATGAAATCATATCATCCTTTGAGGGAGGAGGAGGAGGTGGAAGAGGAGGAGAAGATGGCAGCTGTTGATCAACTGTGTCAACTACTTGTCGGTCACCCATATTTTCAGACCGCTGCGCAGCAGACGGTGAGTGGGAAGAGGTGAACGACGACTGGTTCTGAGGCGGCGCAAGGTGAAGAACGGGATTTCTGTTGGCGTTCTCTTCTCTTCTCAGATGAATCAGGGCGTGGGAATGTATGTGAGATTCTCCGTTTTCTTCCCTCAGTTAGATACGAGAATAAGAGGAAACGAATTCCACA
TARGET=712,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2469
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.243298
PRIMER_LEFT_SEQUENCE=CTCCAAAGCCATAACACATGAA
PRIMER_RIGHT_SEQUENCE=CTAACTGAGGGAAGAAAACGGA
PRIMER_LEFT=497,22
PRIMER_RIGHT=782,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.757
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.2469
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=0.243298
PRIMER_LEFT_1_SEQUENCE=CTCCAAAGCCATAACACATGAA
PRIMER_RIGHT_1_SEQUENCE=TAACTGAGGGAAGAAAACGGAG
PRIMER_LEFT_1=497,22
PRIMER_RIGHT_1=781,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=59.757
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.2469
PRIMER_LEFT_2_PENALTY=0.003589
PRIMER_RIGHT_2_PENALTY=0.243298
PRIMER_LEFT_2_SEQUENCE=CTCCAAAGCCATAACACATGAA
PRIMER_RIGHT_2_SEQUENCE=TCTAACTGAGGGAAGAAAACGG
PRIMER_LEFT_2=497,22
PRIMER_RIGHT_2=783,22
PRIMER_LEFT_2_TM=59.996
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=287
PRIMER_PAIR_PENALTY_3=0.5254
PRIMER_LEFT_3_PENALTY=0.282090
PRIMER_RIGHT_3_PENALTY=0.243298
PRIMER_LEFT_3_SEQUENCE=AGGTGGAAGAGGAGGAGAAGAT
PRIMER_RIGHT_3_SEQUENCE=TAACTGAGGGAAGAAAACGGAG
PRIMER_LEFT_3=548,22
PRIMER_RIGHT_3=781,22
PRIMER_LEFT_3_TM=59.718
PRIMER_RIGHT_3_TM=59.757
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=234
PRIMER_PAIR_PENALTY_4=0.5254
PRIMER_LEFT_4_PENALTY=0.282090
PRIMER_RIGHT_4_PENALTY=0.243298
PRIMER_LEFT_4_SEQUENCE=AGGTGGAAGAGGAGGAGAAGAT
PRIMER_RIGHT_4_SEQUENCE=CTAACTGAGGGAAGAAAACGGA
PRIMER_LEFT_4=548,22
PRIMER_RIGHT_4=782,22
PRIMER_LEFT_4_TM=59.718
PRIMER_RIGHT_4_TM=59.757
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_05853 Ps_contig_mira-and-tgicl-ass_franssen_40220
SEQUENCE=AAGCCGCCGGCGATATTTTTCGTCATGGTACCGCCTCTGCCACCGGTGGAAAACGGAGAATCCACCACGACAGTTTCAATTACAACCCCCGCTATAGAGTCTCAGAGATCTATTCCAACGCCGTTTCTCACCAAAACCTATAACCTAGTTGAGGATAAATCCATCGACGACGTTATTTCATGGAACGAAGATGGATCCACCTTCATCGTTTGGAACCCTACCGTTTTCGCTAGTAATTTGCTTCCAAAATATTTCAAACACAATAATTTCTCAAGCTTCGTTCGTCAACTCAATACTTACGGATTTAGAAAAGTAGTACCTGATCGGTGGGAATTCTCGAACGATTGTTTCCGAAGAGGTGAGAAACGGCTTCTATGCGAGATTCAACGCAGGAAAATCTGTTCTGCCATACCGACGTCAGCGTCTATTGCTACAGTTGCGGCGGTGGAAACCGCGACGGTTGCGGTTCCTTCACCTATGCCGCTGACTGTAGTACCTATGGCGACGCCGATGGTTTCACCGTCGAACTCCGATGAAGAACAAGTGATATCATTGAGTTCGTCTCCGTCGCGAGCGCCATCGGAGCTTCTCGACGAGAACGAGAGATTGAAGAAAGAGAATGTTCTGTTGAACAAGGAGCTTGCTGAGATGAGATTGCTCTGCAACAATATATACACTCTCATGTCAAGTTATGCAAACAACAACAACAATAGCAATAGTGGTAACAATAATATTCAAACGGACGGTGGCGCGCTAAGGRAGCAGAGAGTCCGGAATGACGGCGGCGAAGCCGTTGGATTTGATGCCG
TARGET=697,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05854 Ps_contig_mira-and-tgicl-ass_franssen_40225
SEQUENCE=AGCTCCATTTGCTTNGCTAGGCTTCTCTTCCCAAAATCAAAGTCTTGTTCTTTTCACTTCACCCATTTACCAACTTTCACCAAATGTCTCATCTCTATCTCCTTCCATTTCTCTTTCTTCTATAATACTACTAATAAATTTCATTTCTTCTTCTTCTTCTTCTTCCATTTCTGTGGTTACATCGTAGGACAGGAAGATGTTTTCTAGGTTGTTACGTCCTCACGACCATGAAGGAAGTGTTGGTGTTGTTCATGAAGACACAAATCATCAACACAGCCACCTTGCAGACCCTTGTCTTGTTCTCACTTCTGATCCAAAACCTCGTCTTCGTTGGACTACTGACCTTCACCAACGCTTTGTTGATGCTGTTACCCAACTCGGTGGACCAAGCAAAGCCACACCGAAAGCAATCATGAGGACCATGAATGTCAAGGGTTTGACACTCTATCACTTAAAGAGTCATCTTCAGAAGTACAGGCTTGGTAAACAAGCTGGAAAAGATTCTGATGAGGGATGCAAAGATGGTTCATATCTTCTAGAAAGCCCTGGTACTGAAGACTCATCTCCTAAATTACTAGCTTCTGATGCAAACGAGGGTCATGAAGTCAAGGAGGCTTTAAGAGCCCAGATGGAAGTGCAAAGCAAGCTACATTTACTAGTGGAGGCAGAGAAGCACCTGCTAATTCGCCAAGATGCAGAACGGCGGTATATGGCCATGCTCGAGAGAGCTTGTAAGATGTTGGCTGATCAATTTTATTGGCGAGTCAACTCTAGACACAGACATCCAAAAATATCAAGAACTACCAAGTAACGAACTAGGTGGAATGCATGTTTCAGACATACCGTACATTCTCCAGCCTCAAGGGGCCAACTGTTCGACTGAAAGTTGCCTAACATCCCTTGAGAGTCTAAGAGGGTTGACACTAGAAGGATCACCTGGCGGGACAAAGAGGATGGTGGGATTGGACTCGATGGTATCGCCTTTGATCTGGAGTGAAACTAACATGAGAAGTCAAGGCATTCATTTAGCCCAAGTGAATCCTCAAGGAATAGCAAGGTATGGAATGTAGGTAGGACATTGTAAAGAGAAGACATTATGTTTGACTCCTAATTTGTAGGACTAATTAAGTGCTTAATTTCATGTCTGTTTCCTGTCTTGTATAAGTAGATGGGAATAGAACTAGAATAACCCTCTCTAATTTTGTATATTGGAAATACTTAATAGCTTAATCTGGTAGATTGCCACAATTTACAACC
TARGET=145,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0946
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=CCAACTTTCACCAAATGTCTCA
PRIMER_RIGHT_SEQUENCE=CCTGTCCTACGATGTAACCACA
PRIMER_LEFT=69,22
PRIMER_RIGHT=192,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=0.1555
PRIMER_LEFT_1_PENALTY=0.069179
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=TTTGCTTNGCTAGGCTTCTCTT
PRIMER_RIGHT_1_SEQUENCE=CCTGTCCTACGATGTAACCACA
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=192,22
PRIMER_LEFT_1_TM=60.069
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.50
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.75
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=0.1728
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.164560
PRIMER_LEFT_2_SEQUENCE=CCAACTTTCACCAAATGTCTCA
PRIMER_RIGHT_2_SEQUENCE=TAGTCCAACGAAGACGAGGTTT
PRIMER_LEFT_2=69,22
PRIMER_RIGHT_2=337,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.165
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=0.2343
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.225996
PRIMER_LEFT_3_SEQUENCE=CCAACTTTCACCAAATGTCTCA
PRIMER_RIGHT_3_SEQUENCE=TGAAGGTCAGTAGTCCAACGAA
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=347,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.774
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.2571
PRIMER_LEFT_4_PENALTY=0.008257
PRIMER_RIGHT_4_PENALTY=0.248820
PRIMER_LEFT_4_SEQUENCE=CCAACTTTCACCAAATGTCTCA
PRIMER_RIGHT_4_SEQUENCE=AGACGAGGTTTTGGATCAGAAG
PRIMER_LEFT_4=69,22
PRIMER_RIGHT_4=326,22
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=59.751
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_05855 Ps_contig_mira-and-tgicl-ass_franssen_40232
SEQUENCE=GATCAGCTGCAAATGTTATATGCTATATGTTTACAACAAGTCCAAAATTTGGTGGAAAATCATTGATTGATTATGATATAGCACTTTCATCTGAACCTTGTATGTTACTAGGAGTGAGTGTTGGTGTTATTTGTAACCTTGTTTTTCCAGAATGGTTGATAACGTTAATGTTTGCTGTTTTTCTTGCTTGGTCTACCTCAAAAACATGTAAAAGTGGAGTGATGTTTTGGACCATTGAATCAGAAGAAATAAGGGTTAATGGAGTTGAGGAAATTGAAAAAGGGTTGTTAGAGAAAGAGACTAGTACTAAAGGGTTGGTGAGGTTACTGCAGAAGGAAAACGATAGGTCAGAAAAAGTTGAAGAGAATCTTGTTATGGTCCCTAAAGAAAATAGTGTCAAGTTGTGTATTCCTTGGTTGAAATTGGGAGTGTTACTTTTGGTGTGGCTTTCTTTCTTTTCTATTTATCTTCTTCGCGGCAATGGAAATGGACAGAGAATCATTCCAATGGAACCATGCGGTGTAGGATACTGGATTATCTCATCAGTTCAAGTACCACTAGCAGTTGTTTTCACTGCTTGGATGGTATTTAGAAAAGAAAGTCTTCAAGAGCCGTCTCTTATACCAGAAGTACAATGTCAGAACAGAAATTGTCCATCGAATAAGCTTGTTTTTCCATTGATGGCACTCCTAGCAGGAATACTAGGCGGTGTTTTCGGAATCGGAGGTGGAATGCTTATAAGTCCACTTCTTCTTCAAGTCGGAATAGCTCCTGAGGTAACAGCAGCAACATGTTCTTTCATGGTTTTCTTTTCGTCTACGATGTCGGCGTTGCAGTATCTGTTGTTGGGAATGGAACATGTAGAGACTGCACTTATATTGGCCATAATGTGTTTTGTTGCATCACTTATTGGATTATTGGTTGTGCAGAAAGTAATTGGAAAATATGGAAGACCTTCAATAATAGTGTTTTCAGTTAGCATAGTGATGTCTTTAAGTGTTGTTCTTATGACTAGCTTTGGGGCCATTAAGGTTTGGGAAGATTACAAGTCAGGGAAATATATGGGGTTTAAACTACCTTGTTAATAAAACAGTTGTGTTGTGTTATATGAGTTTTTGTTTANACCTTGCTCTTTCTTATAAGTATTTTTGTTATTTTCACATGTTTGAGAAAAGTTCATAGTNCATTATGTTCTATTTGCNATGAANTACTTTNNGTCNCAANAATT
TARGET=447,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1811
PRIMER_LEFT_PENALTY=0.070748
PRIMER_RIGHT_PENALTY=0.110392
PRIMER_LEFT_SEQUENCE=TGGGAGTGTTACTTTTGGTGTG
PRIMER_RIGHT_SEQUENCE=TCGATGGACAATTTCTGTTCTG
PRIMER_LEFT=423,22
PRIMER_RIGHT=659,22
PRIMER_LEFT_TM=59.929
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=237
PRIMER_PAIR_PENALTY_1=0.1811
PRIMER_LEFT_1_PENALTY=0.070748
PRIMER_RIGHT_1_PENALTY=0.110392
PRIMER_LEFT_1_SEQUENCE=TGGGAGTGTTACTTTTGGTGTG
PRIMER_RIGHT_1_SEQUENCE=CGATGGACAATTTCTGTTCTGA
PRIMER_LEFT_1=423,22
PRIMER_RIGHT_1=658,22
PRIMER_LEFT_1_TM=59.929
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.2399
PRIMER_LEFT_2_PENALTY=0.070748
PRIMER_RIGHT_2_PENALTY=0.169174
PRIMER_LEFT_2_SEQUENCE=TGGGAGTGTTACTTTTGGTGTG
PRIMER_RIGHT_2_SEQUENCE=ACACCGCCTAGTATTCCTGCTA
PRIMER_LEFT_2=423,22
PRIMER_RIGHT_2=711,22
PRIMER_LEFT_2_TM=59.929
PRIMER_RIGHT_2_TM=60.169
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=289
PRIMER_PAIR_PENALTY_3=0.3185
PRIMER_LEFT_3_PENALTY=0.070748
PRIMER_RIGHT_3_PENALTY=0.247716
PRIMER_LEFT_3_SEQUENCE=TGGGAGTGTTACTTTTGGTGTG
PRIMER_RIGHT_3_SEQUENCE=AATCCAGTATCCTACACCGCAT
PRIMER_LEFT_3=423,22
PRIMER_RIGHT_3=535,22
PRIMER_LEFT_3_TM=59.929
PRIMER_RIGHT_3_TM=59.752
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=10.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=0.3403
PRIMER_LEFT_4_PENALTY=0.070748
PRIMER_RIGHT_4_PENALTY=0.269585
PRIMER_LEFT_4_SEQUENCE=TGGGAGTGTTACTTTTGGTGTG
PRIMER_RIGHT_4_SEQUENCE=AGTATCCTACACCGCATGGTTC
PRIMER_LEFT_4=423,22
PRIMER_RIGHT_4=530,22
PRIMER_LEFT_4_TM=59.929
PRIMER_RIGHT_4_TM=60.270
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_05856 Ps_contig_mira-and-tgicl-ass_franssen_40233
SEQUENCE=AAATACATCAACTTTGTAGATATTTTTCATGAAAATTTTTGGAGCATGCTAGCTTCATAAGAAACTACGATCCATTCTCTCGCTCTCTGTGTGTGTGTTTGTGTTTGTTGAATTGTTCGTTCCTCTCTCAATTTTCAGTTTCATACACCAGAAATGGGGTTTCAGAAAATAAGGGTTGACAATCCCATCGTTGAAATGGATGGAGATGAAATGACAAGAGTAATTTGGACAATGATTAAAGATAAGCTTATTTTCCCTTTTTTGGAGCTGGATATTAAGTATTTTGACCTCGGGCTTCCTCATCGAGATGCCACTAATGATAGAGTTACCATTGAAAGTGCCGAAGCTACACTAAAGTATAATGTAGCTATCAAATGTGCAAACTATTAC
TARGET=88,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0718
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.067436
PRIMER_LEFT_SEQUENCE=CTACGATCCATTCTCTCGCTCT
PRIMER_RIGHT_SEQUENCE=CTTGTCATTTCATCTCCATCCA
PRIMER_LEFT=64,22
PRIMER_RIGHT=217,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.933
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=154
PRIMER_PAIR_PENALTY_1=0.0735
PRIMER_LEFT_1_PENALTY=0.006089
PRIMER_RIGHT_1_PENALTY=0.067436
PRIMER_LEFT_1_SEQUENCE=TTGGAGCATGCTAGCTTCATAA
PRIMER_RIGHT_1_SEQUENCE=CTTGTCATTTCATCTCCATCCA
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=217,22
PRIMER_LEFT_1_TM=60.006
PRIMER_RIGHT_1_TM=59.933
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=180
PRIMER_PAIR_PENALTY_2=0.0789
PRIMER_LEFT_2_PENALTY=0.004359
PRIMER_RIGHT_2_PENALTY=0.074581
PRIMER_LEFT_2_SEQUENCE=CTACGATCCATTCTCTCGCTCT
PRIMER_RIGHT_2_SEQUENCE=TAGTGTAGCTTCGGCACTTTCA
PRIMER_LEFT_2=64,22
PRIMER_RIGHT_2=353,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=290
PRIMER_PAIR_PENALTY_3=0.1910
PRIMER_LEFT_3_PENALTY=0.123573
PRIMER_RIGHT_3_PENALTY=0.067436
PRIMER_LEFT_3_SEQUENCE=ACTACGATCCATTCTCTCGCTC
PRIMER_RIGHT_3_SEQUENCE=CTTGTCATTTCATCTCCATCCA
PRIMER_LEFT_3=63,22
PRIMER_RIGHT_3=217,22
PRIMER_LEFT_3_TM=59.876
PRIMER_RIGHT_3_TM=59.933
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=155
PRIMER_PAIR_PENALTY_4=0.1982
PRIMER_LEFT_4_PENALTY=0.123573
PRIMER_RIGHT_4_PENALTY=0.074581
PRIMER_LEFT_4_SEQUENCE=ACTACGATCCATTCTCTCGCTC
PRIMER_RIGHT_4_SEQUENCE=TAGTGTAGCTTCGGCACTTTCA
PRIMER_LEFT_4=63,22
PRIMER_RIGHT_4=353,22
PRIMER_LEFT_4_TM=59.876
PRIMER_RIGHT_4_TM=60.075
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=291
=
PRIMER_SEQUENCE_ID=SSR_05857 Ps_contig_mira-and-tgicl-ass_franssen_40244
SEQUENCE=AATTGCCATAGAAACTCCAGCTGCAATCAGAACCTTATTCTGGGCAATTTGAGCGATATCATTGAAAAAACCAGCACCAAGAGAAGAAGAAACTATAAAGGTAGAAGCTTTAACCTTTCTAGGAAAACGAAAGGAACCCAGTTGTTGGTGAAGACGAAGTTGGTTCCTTTGTTTGAGTGAATGGCTGAAAAGCGAGGAAGGAGTAACACAGAAGCTTGATTGTTGCAACGACATCATCGTCCCAATCAAATTCAACCAGAAAACATGTTACCTTAAGGTACCTACCTACCTTATATTCGGTCAGAATTTGTTTGGTTCCATCAAAGAGGGAATGATGAATGAAAAAGCGTGTCTCTCAGGACGTGGATAATGTATGGGGAGAAAGAACCTATTCCTATTGCTTGCGCAGTTCACAAAGATTTGTGTCTTCTTCCGGTAAAAGCTTTGAAAGAGAGAAATT
TARGET=279,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1297
PRIMER_LEFT_PENALTY=0.014720
PRIMER_RIGHT_PENALTY=0.115007
PRIMER_LEFT_SEQUENCE=CTGAAAAGCGAGGAAGGAGTAA
PRIMER_RIGHT_SEQUENCE=CGCAAGCAATAGGAATAGGTTC
PRIMER_LEFT=184,22
PRIMER_RIGHT=405,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=60.115
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.1719
PRIMER_LEFT_1_PENALTY=0.056850
PRIMER_RIGHT_1_PENALTY=0.115007
PRIMER_LEFT_1_SEQUENCE=AAGCGAGGAAGGAGTAACACAG
PRIMER_RIGHT_1_SEQUENCE=CGCAAGCAATAGGAATAGGTTC
PRIMER_LEFT_1=189,22
PRIMER_RIGHT_1=405,22
PRIMER_LEFT_1_TM=59.943
PRIMER_RIGHT_1_TM=60.115
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=217
PRIMER_PAIR_PENALTY_2=0.2228
PRIMER_LEFT_2_PENALTY=0.107763
PRIMER_RIGHT_2_PENALTY=0.115007
PRIMER_LEFT_2_SEQUENCE=GAAAAGCGAGGAAGGAGTAACA
PRIMER_RIGHT_2_SEQUENCE=CGCAAGCAATAGGAATAGGTTC
PRIMER_LEFT_2=186,22
PRIMER_RIGHT_2=405,22
PRIMER_LEFT_2_TM=59.892
PRIMER_RIGHT_2_TM=60.115
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.2228
PRIMER_LEFT_3_PENALTY=0.107763
PRIMER_RIGHT_3_PENALTY=0.115007
PRIMER_LEFT_3_SEQUENCE=TGAAAAGCGAGGAAGGAGTAAC
PRIMER_RIGHT_3_SEQUENCE=CGCAAGCAATAGGAATAGGTTC
PRIMER_LEFT_3=185,22
PRIMER_RIGHT_3=405,22
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=60.115
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.3071
PRIMER_LEFT_4_PENALTY=0.014720
PRIMER_RIGHT_4_PENALTY=0.292414
PRIMER_LEFT_4_SEQUENCE=CTGAAAAGCGAGGAAGGAGTAA
PRIMER_RIGHT_4_SEQUENCE=CCATACATTATCCACGTCCTGA
PRIMER_LEFT_4=184,22
PRIMER_RIGHT_4=376,22
PRIMER_LEFT_4_TM=60.015
PRIMER_RIGHT_4_TM=59.708
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_05858 Ps_contig_mira-and-tgicl-ass_franssen_40247
SEQUENCE=CGACAGAGAAAGACCTTATCCACATTTCGCTCAACATGACGCTACAGCATCTCTCCAAAGCTCTCTATTCCTCCGCCTCTATCTCTTCCACCGTTGGCATGAACCCTCGCATCCGCGCCGCTTACCAAGACTGTCTCGAACTTCTCGACGACTCCATTGACGCTCTCACTCGCGCATTAACCTCCGTCGTACCTTCCTCCTCCTCCAAAGGCGCCGCTAAGCCTCTTACCTCATCCTCCACCGACGATGTACTCACGTGGCTAAGCGCAGCACTTACCAACCAAGACACGTGCGCTGAAGGTTTTACCGACACTTCCGGTAACGTAAAAGATCAGATGGCGAATAACATAAAGGACTTGTCGGAGCTAGTGAGTAATTGTTTAGCCATTTTCTCTAACTCCGGCGCTGGAGATGATTTCGCCGGTGTTCCTATACATAATAAGAGACGGTTAATGACAATGCCAGAAAATGATTTTCCGATATGGTTGCGAAAACGAGAGAGAAGATTGTTGGGCTTGCCGGTGTCGGCGTTACAGGCTGATGTGATAGTTTCTAAAGATGGTAACGGAACGGTAAAAACCATATCGGAAGCTCTTAAGATGATACCTGAATACGGTAACCGACGCTTCATTATCTATATCAGACAAGGAAGGTATGAAGAAGACAATTTAAAGATCGGAAGGAAGAAGACTAATGTTATGATCATGGGGGACGGAAAGGGAAAGACAGTGATCACAGGGGGACAAAAATGTTATGCAAAAACNTTTTGACCCACATTTTCCAC
TARGET=195,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3281
PRIMER_LEFT_PENALTY=0.130883
PRIMER_RIGHT_PENALTY=0.197222
PRIMER_LEFT_SEQUENCE=CTTATCCACATTTCGCTCAACA
PRIMER_RIGHT_SEQUENCE=TTAGCCACGTGAGTACATCGTC
PRIMER_LEFT=14,22
PRIMER_RIGHT=263,22
PRIMER_LEFT_TM=60.131
PRIMER_RIGHT_TM=60.197
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=250
PRIMER_PAIR_PENALTY_1=0.3578
PRIMER_LEFT_1_PENALTY=0.160609
PRIMER_RIGHT_1_PENALTY=0.197222
PRIMER_LEFT_1_SEQUENCE=ACAGCATCTCTCCAAAGCTCTC
PRIMER_RIGHT_1_SEQUENCE=TTAGCCACGTGAGTACATCGTC
PRIMER_LEFT_1=43,22
PRIMER_RIGHT_1=263,22
PRIMER_LEFT_1_TM=60.161
PRIMER_RIGHT_1_TM=60.197
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.3869
PRIMER_LEFT_2_PENALTY=0.189703
PRIMER_RIGHT_2_PENALTY=0.197222
PRIMER_LEFT_2_SEQUENCE=ATTCCTCCGCCTCTATCTCTTC
PRIMER_RIGHT_2_SEQUENCE=TTAGCCACGTGAGTACATCGTC
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=263,22
PRIMER_LEFT_2_TM=60.190
PRIMER_RIGHT_2_TM=60.197
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=0.4287
PRIMER_LEFT_3_PENALTY=0.130883
PRIMER_RIGHT_3_PENALTY=0.297793
PRIMER_LEFT_3_SEQUENCE=CTTATCCACATTTCGCTCAACA
PRIMER_RIGHT_3_SEQUENCE=GTGTCTTGGTTGGTAAGTGCTG
PRIMER_LEFT_3=14,22
PRIMER_RIGHT_3=288,22
PRIMER_LEFT_3_TM=60.131
PRIMER_RIGHT_3_TM=59.702
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=0.4584
PRIMER_LEFT_4_PENALTY=0.160609
PRIMER_RIGHT_4_PENALTY=0.297793
PRIMER_LEFT_4_SEQUENCE=ACAGCATCTCTCCAAAGCTCTC
PRIMER_RIGHT_4_SEQUENCE=GTGTCTTGGTTGGTAAGTGCTG
PRIMER_LEFT_4=43,22
PRIMER_RIGHT_4=288,22
PRIMER_LEFT_4_TM=60.161
PRIMER_RIGHT_4_TM=59.702
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_05859 Ps_contig_mira-and-tgicl-ass_franssen_40249
SEQUENCE=TAGATAATTAAAAAGTTTAAAGNACAAAAGAAGCTTTCTTTCAAACAGAAATTGCTTTTTACTACTGGCTAGGTTCACTATGCAACAAGTTACTAACAACAACAAAAGAAGTTTAGACATGGAAAATAACCTAAAAAAAAAGTAGATTAAATTTTCTTCTTCTTCTTCTCCCTAACATAATGGCCTTAAAAAACAGTTCACATGAACTGCTAAGAAAAGCTACTAAGTACCAACCACATTAATATAATAATGCAGCTTATTATAATAATTCAATTTTCAATTCTGATTGATCCATTGTTTGTGACCAGGAACCCATTTTGGACATTTGGGACTAAAATAACTTGACATTACCTTTGAGTTCAAGAGTTCAACAACAGGTTCAAACTTGACACACCTTGGTGTCTTGTACTGATTAATTGAAGCACCTAAACTAATGGCATAATCCATTAGTTTATCAAAAGTGCCCTGTTCGACGATCTTTATCTCGAGCGGGCCGATCGATTTGTCCGAGACACGGCCCTGTCGATACACGCTGTTTAGTGACTCTTCAATTGTTAAGCAGCAATCTTCAAAAACACAAGGTGGAATTTGTGTTGATTTGTTCAGTGTGAGTTCCCAGTATAGGACATAGTGTCCTGGAATTGTTGTTGTGTCTGCATAGCTTGTGTATTCCGACACCGTTGCGCCGAATGGTACTAAATGTGTCACAGCATTTGCCATCGCATTCTGAAGCTCAACCTCGTCGGTTTTGTCTGCATCAATGCTCAATACAACGTTTTTTCTGCATACAAAGTTGAACTGTGGTGCTTTGTTCTTGAATCCAGCTACCTTCAGCACATCACCAACTCTATAACGGTATAGTCCAGCATAAGTAGTGACAACAAGTTCATACTCTTGACCGAGTTTGACATCAACAAGTTCAACGAGCTCTTTCTGTTCTTTCTCAATAAGGGGTCTAGGTGTGTGAAGAGAACCAGTGACACCATTGGTTCTATTAACAGGCAAAAACTCATAGTAACACATGGTGGGAATAAGTGTATAAGAAACTTCACTAGGTTTACAAAGAGGGTTAAGATTGACACCAAAGTAACATTCACTAGAAGCATACATAGTGCAAACAAGTGGTAAACCATTGCTATAATGATCCAAAATAGGAATATACTGTGACATTGTTCCTGTGACAATAACATCCACATACTTAGTATTAGGCCACAACCTAGTAATAATCCCCTGCCAAGAACTCTTACTACACTCATTTTGAATAAAATCAGCAAGCTTAGGATCAGGTTTAAGGATTTTCATCACAGAATCTCTCACTGTTATGTCAGTGATCAAAGAGTTAACTGTCCCTGTTCTAATATCATTTGAAAGAAGGGTCCAGTGTTTTTCAAGAAACCTAATAGCCCTTATGAAACCAGAGGCAAAAACAGCACCAACCCTTAGAACCTCTTTGTTTTGGCAAAGACCACAAAGGAGTTGAGAATACATGCTTTGATATGAATCAGGACAGAGCACAGTTTCATTTGGGCTTGTGTAGTTTGTGTAAGGGTCATAAGGTCTATCTCTAAAATAGGAACTTTTGTAGTAGCTTGTTAAAACAGGCCTAGCTACAATTCCACCAGGTGTTGTAGCCTCAGATTTTATAAACATTAGGTACATTCCTTTCCCTTTTTCTAATCCAGGAACAAACTCACTCATCACTGGCATCAATAAACTATACAACAAACTTCTCCTTCCTAACTCCTCTTCAATTGTTGGCATAAGTTTTCTCTCACCACCAGATGTACCAGAACTTGTGAGAAACTCTGAAATGGGTTTGGAGCAAAGGATGGGAGAGGTGTCACCATTTGCGATGCGTTCGATATCGGGTTGAATATCTTCGTATGAGATAACAGGTAAGTAGTTTTTTGAAGGTATCACGGTTTGTGTTACCGTTGAGTATTGTTACCGTTTGAGATACTCAACGT
TARGET=154,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6177
PRIMER_LEFT_PENALTY=0.688571
PRIMER_RIGHT_PENALTY=0.929153
PRIMER_LEFT_SEQUENCE=TGGCTAGGTTCACTATGCAACA
PRIMER_RIGHT_SEQUENCE=TAGTCCCAAATGTCCAAAATGG
PRIMER_LEFT=65,22
PRIMER_RIGHT=333,22
PRIMER_LEFT_TM=60.689
PRIMER_RIGHT_TM=60.929
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=269
PRIMER_PAIR_PENALTY_1=1.6354
PRIMER_LEFT_1_PENALTY=0.706271
PRIMER_RIGHT_1_PENALTY=0.929153
PRIMER_LEFT_1_SEQUENCE=GGCTAGGTTCACTATGCAACAA
PRIMER_RIGHT_1_SEQUENCE=TAGTCCCAAATGTCCAAAATGG
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=333,22
PRIMER_LEFT_1_TM=59.294
PRIMER_RIGHT_1_TM=60.929
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=268
PRIMER_PAIR_PENALTY_2=1.9137
PRIMER_LEFT_2_PENALTY=0.688571
PRIMER_RIGHT_2_PENALTY=1.225160
PRIMER_LEFT_2_SEQUENCE=TGGCTAGGTTCACTATGCAACA
PRIMER_RIGHT_2_SEQUENCE=TCCTGGTCACAAACAATGGAT
PRIMER_LEFT_2=65,22
PRIMER_RIGHT_2=309,21
PRIMER_LEFT_2_TM=60.689
PRIMER_RIGHT_2_TM=60.225
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=1.9314
PRIMER_LEFT_3_PENALTY=0.706271
PRIMER_RIGHT_3_PENALTY=1.225160
PRIMER_LEFT_3_SEQUENCE=GGCTAGGTTCACTATGCAACAA
PRIMER_RIGHT_3_SEQUENCE=TCCTGGTCACAAACAATGGAT
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=309,21
PRIMER_LEFT_3_TM=59.294
PRIMER_RIGHT_3_TM=60.225
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=244
PRIMER_PAIR_PENALTY_4=1.9502
PRIMER_LEFT_4_PENALTY=0.688571
PRIMER_RIGHT_4_PENALTY=1.261614
PRIMER_LEFT_4_SEQUENCE=TGGCTAGGTTCACTATGCAACA
PRIMER_RIGHT_4_SEQUENCE=GTTCCTGGTCACAAACAATGG
PRIMER_LEFT_4=65,22
PRIMER_RIGHT_4=311,21
PRIMER_LEFT_4_TM=60.689
PRIMER_RIGHT_4_TM=60.262
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_05860 Ps_contig_mira-and-tgicl-ass_franssen_40256
SEQUENCE=AAAAAGTTTCTATTCAAATGATTTAATACAAATATTGCATTTACATAATGAAATAGACCTCATTAATAATACCAACATCTTAAATATAAGTTGCTAATAAAACAATAAAACATTGATTACACATGAATCAATCAAGAAGTCTTAATAAGGGCTTTCAAATACACTAGTTCATCATGGCTAGCTAGCACACATTTTATTCAGGAATTATATATGAAGTATAGCATCATGAGCAATCAGCCTTAATTTTTTTGTAGCAGATTGACTGCTCATGATGTTATATCAATTTCCATGAATTTAATTACCTATAGTCATGCTAGCATATTAATTAATATCTATATGCTGCCCCAATAATGTAAATTTTGGTCTCTACCGAAAGGCTCCTCCAAGGGCAGGTGCCCAATAATCAGCTCCATTGTCACTCCCAACTTGAAGTGTGCATGACACTGGAACAAGACACAGCCCTCTACTTTGTAGGTCCTTGTTTGGTTCTTCATGAGAAACCTCTTCAACTGCTTTCCTCTTCAAGCTATTTTCATGTAGCAACTGACCAGGATCTTCAGGAAATAAACAATTCTTATCTCCTTGAACTGAATGTGGTTGTCTTGTGTTTCCAGATCCATTGCTCAAGTATGGTAAGCTAAGAGCCTCAATTTGTGTCTGAAGGAATCTGATATAACCAATAGCTTCTAACAAGACAGAGGCTGTGTCAGTCTTTCCAAATGGGGAAACAAGCTGATGAAGGGCAGTAATTCTGTCACCTAATTTCTCCTTCCTAACCTTGAAGGTTGATTGAGTTGCAGCTTGTTGAACCCTAGCTTTTTTCATTGCCCCACCAGATGCAGTGCTGTTGCACTCAGAAGATAGATCTGGTGGTGGATGCCTCAAATCTGTATTATTATTGTTAGAGAAATCTAACATGTTGGTGTTGAAACTTGTGACACATGAATTAAGTTCTTCATTTCCATGTCCATATGCATAGCTATTTCCTGAGCTTTCTTGTTTAACATCAACAATAGAAGAATTTGGAGCTTGAGTTATTAGCATTTGATTTTGAAATTGGCTCAATCCAATCTTATCTTCCTCACCAACAATTCCACTCATGAGTAGTTGGCTCCAAGATTCAGGAAGCTCTTGATGATAATCATGCCAAGAAGGAAAAGTAAGAGAAGAAGAAGAATTTGGTGTAAAATTAGGGATGAGAAAGTTAGAATTAGGAGTCGAAAAGAAAGGAGGAGGTTGTGGAGACGGAGGTCTCATGTTATTGATGTTCCACCAATTAGGGTTACTAGCCATCATTTGTTGAACTGGTGAGCTTTGTACAACACCTCTATTCATGCCTAAAGCTTCTTCAACTCTTAAGAATTTTATTACAAAAAGAAGAAGAAAATAAAAGTGAGAAATTATTTTTCCTCTCTCTCTCTTTTCAACCCTAAACCGTCTTNNGAATTTGNGGGTNTTNTGAGGTGATAGTAGGGACA
TARGET=1165,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1282
PRIMER_LEFT_PENALTY=0.124655
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=TCATGAGTAGTTGGCTCCAAGA
PRIMER_RIGHT_SEQUENCE=CCAGTTCAACAAATGATGGCTA
PRIMER_LEFT=1097,22
PRIMER_RIGHT=1307,22
PRIMER_LEFT_TM=59.875
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=0.1339
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=GTAGTTGGCTCCAAGATTCAGG
PRIMER_RIGHT_1_SEQUENCE=CCAGTTCAACAAATGATGGCTA
PRIMER_LEFT_1=1103,22
PRIMER_RIGHT_1=1307,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=205
PRIMER_PAIR_PENALTY_2=0.2334
PRIMER_LEFT_2_PENALTY=0.124655
PRIMER_RIGHT_2_PENALTY=0.108707
PRIMER_LEFT_2_SEQUENCE=TCATGAGTAGTTGGCTCCAAGA
PRIMER_RIGHT_2_SEQUENCE=AACCTCCTCCTTTCTTTTCGAC
PRIMER_LEFT_2=1097,22
PRIMER_RIGHT_2=1237,22
PRIMER_LEFT_2_TM=59.875
PRIMER_RIGHT_2_TM=60.109
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=141
PRIMER_PAIR_PENALTY_3=0.2391
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.108707
PRIMER_LEFT_3_SEQUENCE=GTAGTTGGCTCCAAGATTCAGG
PRIMER_RIGHT_3_SEQUENCE=AACCTCCTCCTTTCTTTTCGAC
PRIMER_LEFT_3=1103,22
PRIMER_RIGHT_3=1237,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=60.109
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=0.3054
PRIMER_LEFT_4_PENALTY=0.124655
PRIMER_RIGHT_4_PENALTY=0.180765
PRIMER_LEFT_4_SEQUENCE=TCATGAGTAGTTGGCTCCAAGA
PRIMER_RIGHT_4_SEQUENCE=ACAAAGCTCACCAGTTCAACAA
PRIMER_LEFT_4=1097,22
PRIMER_RIGHT_4=1317,22
PRIMER_LEFT_4_TM=59.875
PRIMER_RIGHT_4_TM=59.819
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=221
=
PRIMER_SEQUENCE_ID=SSR_05861 Ps_contig_mira-and-tgicl-ass_franssen_40256
SEQUENCE=AAAAAGTTTCTATTCAAATGATTTAATACAAATATTGCATTTACATAATGAAATAGACCTCATTAATAATACCAACATCTTAAATATAAGTTGCTAATAAAACAATAAAACATTGATTACACATGAATCAATCAAGAAGTCTTAATAAGGGCTTTCAAATACACTAGTTCATCATGGCTAGCTAGCACACATTTTATTCAGGAATTATATATGAAGTATAGCATCATGAGCAATCAGCCTTAATTTTTTTGTAGCAGATTGACTGCTCATGATGTTATATCAATTTCCATGAATTTAATTACCTATAGTCATGCTAGCATATTAATTAATATCTATATGCTGCCCCAATAATGTAAATTTTGGTCTCTACCGAAAGGCTCCTCCAAGGGCAGGTGCCCAATAATCAGCTCCATTGTCACTCCCAACTTGAAGTGTGCATGACACTGGAACAAGACACAGCCCTCTACTTTGTAGGTCCTTGTTTGGTTCTTCATGAGAAACCTCTTCAACTGCTTTCCTCTTCAAGCTATTTTCATGTAGCAACTGACCAGGATCTTCAGGAAATAAACAATTCTTATCTCCTTGAACTGAATGTGGTTGTCTTGTGTTTCCAGATCCATTGCTCAAGTATGGTAAGCTAAGAGCCTCAATTTGTGTCTGAAGGAATCTGATATAACCAATAGCTTCTAACAAGACAGAGGCTGTGTCAGTCTTTCCAAATGGGGAAACAAGCTGATGAAGGGCAGTAATTCTGTCACCTAATTTCTCCTTCCTAACCTTGAAGGTTGATTGAGTTGCAGCTTGTTGAACCCTAGCTTTTTTCATTGCCCCACCAGATGCAGTGCTGTTGCACTCAGAAGATAGATCTGGTGGTGGATGCCTCAAATCTGTATTATTATTGTTAGAGAAATCTAACATGTTGGTGTTGAAACTTGTGACACATGAATTAAGTTCTTCATTTCCATGTCCATATGCATAGCTATTTCCTGAGCTTTCTTGTTTAACATCAACAATAGAAGAATTTGGAGCTTGAGTTATTAGCATTTGATTTTGAAATTGGCTCAATCCAATCTTATCTTCCTCACCAACAATTCCACTCATGAGTAGTTGGCTCCAAGATTCAGGAAGCTCTTGATGATAATCATGCCAAGAAGGAAAAGTAAGAGAAGAAGAAGAATTTGGTGTAAAATTAGGGATGAGAAAGTTAGAATTAGGAGTCGAAAAGAAAGGAGGAGGTTGTGGAGACGGAGGTCTCATGTTATTGATGTTCCACCAATTAGGGTTACTAGCCATCATTTGTTGAACTGGTGAGCTTTGTACAACACCTCTATTCATGCCTAAAGCTTCTTCAACTCTTAAGAATTTTATTACAAAAAGAAGAAGAAAATAAAAGTGAGAAATTATTTTTCCTCTCTCTCTCTTTTCAACCCTAAACCGTCTTNNGAATTTGNGGGTNTTNTGAGGTGATAGTAGGGACA
TARGET=1410,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05862 Ps_contig_mira-and-tgicl-ass_franssen_40263
SEQUENCE=TTCTTTTGTTAATNGAATAGCTTTCACTCTAAAAAGCCACTTTGTTTCTTCAACATATTGTTGCAAGAAAGTGTTGTTAAACTTTATGGCACCTTCTTTTTCTTCTTCTTCCTTTCCTCTTCGTTGGGAGAGCACAGGAGATCAATGGTGGTATGCAACACCTATTGATTTAGCAGCTGCAAATGGTCTCTATGATTTAGTCCTTGAGCTTCTTCATCTTGATACAAATCTTCTCATTAAACTCACTTCTCTTCGTCGAATTCGCCGACTCGAGACTGTGTGGGATGATGAATCTCAGTTTGAAGATGTTGCCAAGTGCAGGTCTTATGTTGCAAGGAAGCTTATGGTTGAGTGTGAGAAAACAGCAAGAGGAGAGAATTCTCTGGTTGGTAGTGGATATGGTGGTTGGCTTTTGTACACTGCTGCTTCTGCTGGTGATTTGGAGTTTGTTCGTGAATTGTTGAG
TARGET=100,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.2105
PRIMER_LEFT_PENALTY=2.953655
PRIMER_RIGHT_PENALTY=0.256829
PRIMER_LEFT_SEQUENCE=AGCTTTCACTCTAAAAAGCCAC
PRIMER_RIGHT_SEQUENCE=TGTTGCATACCACCATTGATCT
PRIMER_LEFT=18,22
PRIMER_RIGHT=159,22
PRIMER_LEFT_TM=57.046
PRIMER_RIGHT_TM=60.257
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=142
PRIMER_PAIR_PENALTY_1=3.2105
PRIMER_LEFT_1_PENALTY=2.953655
PRIMER_RIGHT_1_PENALTY=0.256829
PRIMER_LEFT_1_SEQUENCE=GCTTTCACTCTAAAAAGCCACT
PRIMER_RIGHT_1_SEQUENCE=TGTTGCATACCACCATTGATCT
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=159,22
PRIMER_LEFT_1_TM=57.046
PRIMER_RIGHT_1_TM=60.257
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=3.2373
PRIMER_LEFT_2_PENALTY=2.953655
PRIMER_RIGHT_2_PENALTY=0.283676
PRIMER_LEFT_2_SEQUENCE=AGCTTTCACTCTAAAAAGCCAC
PRIMER_RIGHT_2_SEQUENCE=TTGGCAACATCTTCAAACTGAG
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=314,22
PRIMER_LEFT_2_TM=57.046
PRIMER_RIGHT_2_TM=60.284
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=3.2373
PRIMER_LEFT_3_PENALTY=2.953655
PRIMER_RIGHT_3_PENALTY=0.283676
PRIMER_LEFT_3_SEQUENCE=AGCTTTCACTCTAAAAAGCCAC
PRIMER_RIGHT_3_SEQUENCE=CTTGGCAACATCTTCAAACTGA
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=315,22
PRIMER_LEFT_3_TM=57.046
PRIMER_RIGHT_3_TM=60.284
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=3.2373
PRIMER_LEFT_4_PENALTY=2.953655
PRIMER_RIGHT_4_PENALTY=0.283676
PRIMER_LEFT_4_SEQUENCE=GCTTTCACTCTAAAAAGCCACT
PRIMER_RIGHT_4_SEQUENCE=CTTGGCAACATCTTCAAACTGA
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=315,22
PRIMER_LEFT_4_TM=57.046
PRIMER_RIGHT_4_TM=60.284
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=297
=
PRIMER_SEQUENCE_ID=SSR_05863 Ps_contig_mira-and-tgicl-ass_franssen_40264
SEQUENCE=TACNCGTCGGGGCTCCCTGCTCTTTCCAACTCCTTCTCTCTCTCTCAACTTACCTATCTATGGCTTCTAACTATTCATTCTCTTCTTCAATTACTTCTCCAAAATCTAGCATGTTTTACTATGGTGGAAGTGAAGATTTCTATGACCAACCTCACTTCCTCCAAGCTTGTTTTCTTTGTAGAAAACATCTTGGCCAAAACAAAGACATCTTTATGTACAGAGGGAACACACCATTTTGTAGCAAAGAGTGCAGAGAAGAACAAATAGAGATTGATGAATCTAAAGAGAAAAGTTGGAAGATTTCATCCAAAAGAAGTGTTAGAAATTCGGAAACCAATCAGAATTCTACAAACAACAAGACTGTAAGATCAGAATCAGTTGCAGTTGCCTAATTCACATGATATGTATGGTGAAAAAATAATTCCAAATTTTTGAGAAAAAATGATGAAGTGGAAAATTAAGAGGAATTAATTTCCATGATGAGATTCTGATTTTAAAAAAGATGAATCTTTGTACCTTTTGTAAAGAAATTGTTGGAGTTTTAATGAGAGATAGAAAGAACAAAAAAACTCAACAAGGAATTTGGTCTTTTGTTGTGTATTTATTGATCYTTNTANGATTTTCNCATATTTATATNTAGTTC
TARGET=35,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05864 Ps_contig_mira-and-tgicl-ass_franssen_40266
SEQUENCE=ATAAGCTAAGATGACAGAGGTGAAACTGCATGGATTTTGGTATAGTCCTTTTACTTTTAGGGTAGTATGGACCCTAAATCTGAAGGGTATACCATATGAGCACATAGAAGAAGATCGTTTCAATAAGAGTCCTCAACAACAACAACCATCTTCATCGTACATATTACTGTT
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05865 Ps_contig_mira-and-tgicl-ass_franssen_40271
SEQUENCE=AGAAGATACTTGATTTAATCTCCAACAGTTTTCAACACTTTCATGACAATCTGTTTTTGTTTTTTACTTGAAAAAATCAGGCAAGAGTGCTTACACTTCACAATCTCTCAAACAACACAAACATCACTCTTCCCAACAACACTCTTCCCAGGACAATACAAAACAAAACCAAGAAAGAAAGAACGCGGGAGAAATAAAACAATAAAAAGAACATAAATCCTTCAAATGGATTGGTCTTCATCAACTGGAGATAACTATAGCTGCAACTAAACCAACAAAAAGGGACACAAACATTGTTCCTTGAAGGCTAATCAAATTGATAGCACCAGAAGTAACCTTAGTTGTCTCACCCTGATCATCATCGGAAGATGAATCCTTAGCAGATTTAGGTGCTTTTGCTATAGTAGGAGCAGGAGCGAGAGCTTTAGGCTTAGAGAAATCCAGAGGAATAAGCACCTTATCAACATGATATATAGCAAGTGTCTTATCAGAATACACAATCCCTGTGAGTGTAGCATTAACAGACCCAGTTGAAATATTCACATTGTTTCCATAAGCTGTCACATTCATTTGATAACCAGAAGGACTATCACTTGCAAGAGTGAGCACTGGGTTACTCAGTGAATCAAAGTTTGAGCTTGAGACATAAACAGGAAGAACATGAAACTGTATCAGTTCTTTCTGTTGGCGTTCGCCGAGGGAATTGAAGTATCCGGCTTTGAGCTGGGAAAAAGCACCGTCATCGGGTGCAAGAATAGTTAAACCACCGGATTTTGTTGTCACTAGTTGTGCATTGATTTGGTTAATGATTTGTGTTGTTTTCAGCAACCGGATCAGTGTGTTGAATGATTTAGCTTTTCTTAGGATTTTGATAATGTCTTGACCGGACGAAGAATCGGAGGAGGGTGATTGAGGTAATGAAGGGACTAATGGTTTGCCGGTGCTGTTGCTGGAGCAGGTGTGGCGAGGGGCTAGGTTTTGCGTGGGTAGGGTTTAGGNGCTGCTGCGAGGTGATTGGAGCTAGGAA
TARGET=171,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0695
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.061230
PRIMER_LEFT_SEQUENCE=CAAACATCACTCTTCCCAACAA
PRIMER_RIGHT_SEQUENCE=TGCTAAGGATTCATCTTCCGAT
PRIMER_LEFT=117,22
PRIMER_RIGHT=381,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.061
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.0745
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.066262
PRIMER_LEFT_1_SEQUENCE=CAAACATCACTCTTCCCAACAA
PRIMER_RIGHT_1_SEQUENCE=GATGATGATCAGGGTGAGACAA
PRIMER_LEFT_1=117,22
PRIMER_RIGHT_1=362,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=59.934
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=0.0956
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.087340
PRIMER_LEFT_2_SEQUENCE=CAAACATCACTCTTCCCAACAA
PRIMER_RIGHT_2_SEQUENCE=CCCTTTTTGTTGGTTTAGTTGC
PRIMER_LEFT_2=117,22
PRIMER_RIGHT_2=283,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=59.913
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.1386
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=CAAACATCACTCTTCCCAACAA
PRIMER_RIGHT_3_SEQUENCE=GGAACAATGTTTGTGTCCCTTT
PRIMER_LEFT_3=117,22
PRIMER_RIGHT_3=299,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.1607
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.061230
PRIMER_LEFT_4_SEQUENCE=ACAACACTCTTCCCAGGACAAT
PRIMER_RIGHT_4_SEQUENCE=TGCTAAGGATTCATCTTCCGAT
PRIMER_LEFT_4=135,22
PRIMER_RIGHT_4=381,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=60.061
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=247
=
PRIMER_SEQUENCE_ID=SSR_05866 Ps_contig_mira-and-tgicl-ass_franssen_40288
SEQUENCE=AGGTATAAGTCTATGTTTAAATTTATTTATAACAAATGCATTGGTTTTGAGGCTGGAACGGTGATCTCATCCAAACTTGTTGATAGTAAAATTTGAAGAGAATTATTACATGGAAACAACATGCAATCCTACAAAATAGATTGCAATCATAAGAAGCTAGTGTCTATGAAATAATTTCATTTGTTTAACTTATACATGTTTTCTCCTTTGAAAAAATGTTTGACTAAATCCTAGATAGTAAAATGAAGATATAAGGGCCCTTATTTTCCACAATTTCAACTGCATTTGGTCCATGTAATTGATTGAGCTCATGATGACTCTAAACTTTTGACGAACTTTTTAGTCTGTTCTAGGGTTAGAAACATGATCACGTTCCAAGATCCTAGTCGTCCAAAATTTGGGATGAACCCTTTATAAAATGCCAAGGGTCCATCATTCTTTAAGGTTTTGACAAAACAATCAAGGGTGCTTTTGTAGCTAGAATCTCCCATCATTCTCGACTTGACCACATCAACTGGGGAGCCGATACAAACGGCGAAAAACCCTGCCCCAAGGCCAGCAAAGAGATGAGTCACAACATTGTCGGTGAATCCTGGAATTTTCAAAATCGTCTGCTTCACTTGATCATAGCTAGCTAGTTCAGCAGCGTTGATAATACCATTTCTTGCTATGTTGGGGCCAAGCCCTGTCCAAAGAGCTCTAACTCCTTCCTGTCTCGCGATTGACGAATAAGCATTTAAAGATCCAGAGTAGCGCCTGGGAACTCCAGGAGGTAATTTTCCTTCTGCTTGAAGTCTGACTTTGACAAGATCAGTTGGATTCGCCACTGTAATTGCCACGGCACCAGTCGTAAATGCAGCTAGGATTTTCTTTGACAGAGGAACATCACCAACATGGTCACTTCCCGTATAGAAAGTCTTAACAGGCTCGTATAACCCAATTCTTAATCCTCCATACAAACACTGACGATGTAGTCCTGGCACAATGCCCTTCCAGAGCGCTGAAAGACCTTCTTCCCTGGCAATGGTTCCAACCGTTCCCAGCAATCCCTTGTATTTAGGTAAGGAGGATAGATCACCAGCTACAGCTTGTTTTTGAAGCTGAAGCCTAACTTTGGCAGTGTCCAAAGGTATGGTACAGACCTCAGCGAAACATGCGGAGAAAGCGCTGCTGGCAAAGGTTCCAGCAAATGAGAGGTCAGATTTGGACTTGGAATCAGCAACCATGGCAATGATTCAGAGAAAGAAGAAGAAGAAGGTTGCGTTGGGTTTGGGAAAAGATGATTTGGGTTGTTCCTTTTTGTTTTGGTTTGATCACTTTTGAAATAAGCGCTTAGAAAGAGTCATGTATTTGGAAATT
TARGET=1243,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2881
PRIMER_LEFT_PENALTY=0.022786
PRIMER_RIGHT_PENALTY=1.265349
PRIMER_LEFT_SEQUENCE=CACCAGCTACAGCTTGTTTTTG
PRIMER_RIGHT_SEQUENCE=ACAACCCAAATCATCTTTTCCC
PRIMER_LEFT=1075,22
PRIMER_RIGHT=1292,22
PRIMER_LEFT_TM=59.977
PRIMER_RIGHT_TM=61.265
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=1.3755
PRIMER_LEFT_1_PENALTY=0.110131
PRIMER_RIGHT_1_PENALTY=1.265349
PRIMER_LEFT_1_SEQUENCE=AGAGGTCAGATTTGGACTTGGA
PRIMER_RIGHT_1_SEQUENCE=ACAACCCAAATCATCTTTTCCC
PRIMER_LEFT_1=1192,22
PRIMER_RIGHT_1=1292,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=61.265
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=1.4072
PRIMER_LEFT_2_PENALTY=0.141882
PRIMER_RIGHT_2_PENALTY=1.265349
PRIMER_LEFT_2_SEQUENCE=CCCAGCAATCCCTTGTATTTAG
PRIMER_RIGHT_2_SEQUENCE=ACAACCCAAATCATCTTTTCCC
PRIMER_LEFT_2=1038,22
PRIMER_RIGHT_2=1292,22
PRIMER_LEFT_2_TM=59.858
PRIMER_RIGHT_2_TM=61.265
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=1.4072
PRIMER_LEFT_3_PENALTY=0.141882
PRIMER_RIGHT_3_PENALTY=1.265349
PRIMER_LEFT_3_SEQUENCE=CCCAGCAATCCCTTGTATTTAG
PRIMER_RIGHT_3_SEQUENCE=ATCATCTTTTCCCAAACCCAAC
PRIMER_LEFT_3=1038,22
PRIMER_RIGHT_3=1283,22
PRIMER_LEFT_3_TM=59.858
PRIMER_RIGHT_3_TM=61.265
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=1.4072
PRIMER_LEFT_4_PENALTY=0.141882
PRIMER_RIGHT_4_PENALTY=1.265349
PRIMER_LEFT_4_SEQUENCE=CCAGCAATCCCTTGTATTTAGG
PRIMER_RIGHT_4_SEQUENCE=ACAACCCAAATCATCTTTTCCC
PRIMER_LEFT_4=1039,22
PRIMER_RIGHT_4=1292,22
PRIMER_LEFT_4_TM=59.858
PRIMER_RIGHT_4_TM=61.265
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.5000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_05867 Ps_contig_mira-and-tgicl-ass_franssen_40291
SEQUENCE=GGGTCCCGTAAAATNGAAATAATCNACATATACAAACCTACCGAATCAAAACCTGTGGTTACTCCGGTGGAGCGATCGCAATAGAAGAAGAAGATGTCGACGGTGTACGTGTTGGAACCGCCGACAAAGGGAAAAGTGGTCGTGAACACAACGCGCGGCCCTATCGATATCGAGCTATGGCCTAAAGAAGCTCCCAAAGCCGTAAGGAACTTCGTTCAACTCTGCCTCGAAGGTTATTACGACAACACCATCTTCCACCGTGTCATCAAAGACTTCCTCGTTCAGTGTGGCGACCCCACTGGAACTGGCACCGGAGGTGAAAGTATTTATGGAGATGTTTTTGCTGATGAGTTTCATTCGCGTATTAAATTNAAAATTCAAGCATAGAGGTTTGGTGGCAAT
TARGET=83,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3129
PRIMER_LEFT_PENALTY=0.274768
PRIMER_RIGHT_PENALTY=0.038090
PRIMER_LEFT_SEQUENCE=ATCAAAACCTGTGGTTACTCCG
PRIMER_RIGHT_SEQUENCE=AGGAAGTCTTTGATGACACGGT
PRIMER_LEFT=44,22
PRIMER_RIGHT=277,22
PRIMER_LEFT_TM=60.275
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=9.3000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.3129
PRIMER_LEFT_1_PENALTY=0.274768
PRIMER_RIGHT_1_PENALTY=0.038090
PRIMER_LEFT_1_SEQUENCE=CTACCGAATCAAAACCTGTGGT
PRIMER_RIGHT_1_SEQUENCE=AGGAAGTCTTTGATGACACGGT
PRIMER_LEFT_1=37,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=60.275
PRIMER_RIGHT_1_TM=60.038
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.3769
PRIMER_LEFT_2_PENALTY=0.274768
PRIMER_RIGHT_2_PENALTY=0.102129
PRIMER_LEFT_2_SEQUENCE=ACCTACCGAATCAAAACCTGTG
PRIMER_RIGHT_2_SEQUENCE=GTGGAAGATGGTGTTGTCGTAA
PRIMER_LEFT_2=35,22
PRIMER_RIGHT_2=257,22
PRIMER_LEFT_2_TM=60.275
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.3769
PRIMER_LEFT_3_PENALTY=0.274768
PRIMER_RIGHT_3_PENALTY=0.102129
PRIMER_LEFT_3_SEQUENCE=CTACCGAATCAAAACCTGTGGT
PRIMER_RIGHT_3_SEQUENCE=GTGGAAGATGGTGTTGTCGTAA
PRIMER_LEFT_3=37,22
PRIMER_RIGHT_3=257,22
PRIMER_LEFT_3_TM=60.275
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.3769
PRIMER_LEFT_4_PENALTY=0.274768
PRIMER_RIGHT_4_PENALTY=0.102129
PRIMER_LEFT_4_SEQUENCE=ATCAAAACCTGTGGTTACTCCG
PRIMER_RIGHT_4_SEQUENCE=GTGGAAGATGGTGTTGTCGTAA
PRIMER_LEFT_4=44,22
PRIMER_RIGHT_4=257,22
PRIMER_LEFT_4_TM=60.275
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_05868 Ps_contig_mira-and-tgicl-ass_franssen_40301
SEQUENCE=GGAGAGTGGGAGTGGATGGACATGGATTGAGAGGAGAGAGATTGACATTGGAAAATGGGGATTAAGGCGTGAGACTATACGAGAAGAGAGGAGAGTGCAAATTTCCCCTAACCAAATCCACCATTTCTGAACCAACCAACCAACCACCACCACCACTTCCATCTTCTCTCTCACGCACACATCATCATCATCAAGAAATCAATTTTTCTCTCTGAATCGAGGGAAGTGTTAGGTTTTTTTTGGAGAAGAGGGGAATATGCAACAAAGACTCAAGTTGCAGCAACAACAACAAGCACTCATGCAGCAAGCTTTGCTTCAGCAACAGCAGATGTACCATCACCCTGGCATGCTTGCCGCTGCCGCTATGACTCAGATGGAGCCTGTACCAAGCGGAAATCTACCTCCTGGTTTTGATGCTTCTGCATGCCGTAGTGTTTATGTAGGAAATATTCATGTAAAT
TARGET=130,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2859
PRIMER_LEFT_PENALTY=0.283555
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=AATTTCCCCTAACCAAATCCAC
PRIMER_RIGHT_SEQUENCE=ACCTAACACTTCCCTCGATTCA
PRIMER_LEFT=99,22
PRIMER_RIGHT=233,22
PRIMER_LEFT_TM=60.284
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=0.3177
PRIMER_LEFT_1_PENALTY=0.283555
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=AATTTCCCCTAACCAAATCCAC
PRIMER_RIGHT_1_SEQUENCE=TTGTTGCATATTCCCCTCTTCT
PRIMER_LEFT_1=99,22
PRIMER_RIGHT_1=264,22
PRIMER_LEFT_1_TM=60.284
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=166
PRIMER_PAIR_PENALTY_2=0.3177
PRIMER_LEFT_2_PENALTY=0.283555
PRIMER_RIGHT_2_PENALTY=0.034102
PRIMER_LEFT_2_SEQUENCE=AATTTCCCCTAACCAAATCCAC
PRIMER_RIGHT_2_SEQUENCE=TCTTTGTTGCATATTCCCCTCT
PRIMER_LEFT_2=99,22
PRIMER_RIGHT_2=267,22
PRIMER_LEFT_2_TM=60.284
PRIMER_RIGHT_2_TM=59.966
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.3410
PRIMER_LEFT_3_PENALTY=0.283555
PRIMER_RIGHT_3_PENALTY=0.057411
PRIMER_LEFT_3_SEQUENCE=AATTTCCCCTAACCAAATCCAC
PRIMER_RIGHT_3_SEQUENCE=GGTACATCTGCTGTTGCTGAAG
PRIMER_LEFT_3=99,22
PRIMER_RIGHT_3=334,22
PRIMER_LEFT_3_TM=60.284
PRIMER_RIGHT_3_TM=59.943
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.3792
PRIMER_LEFT_4_PENALTY=0.283555
PRIMER_RIGHT_4_PENALTY=0.095684
PRIMER_LEFT_4_SEQUENCE=AATTTCCCCTAACCAAATCCAC
PRIMER_RIGHT_4_SEQUENCE=TTGCTGCAACTTGAGTCTTTGT
PRIMER_LEFT_4=99,22
PRIMER_RIGHT_4=282,22
PRIMER_LEFT_4_TM=60.284
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=184
=
PRIMER_SEQUENCE_ID=SSR_05869 Ps_contig_mira-and-tgicl-ass_franssen_40301
SEQUENCE=GGAGAGTGGGAGTGGATGGACATGGATTGAGAGGAGAGAGATTGACATTGGAAAATGGGGATTAAGGCGTGAGACTATACGAGAAGAGAGGAGAGTGCAAATTTCCCCTAACCAAATCCACCATTTCTGAACCAACCAACCAACCACCACCACCACTTCCATCTTCTCTCTCACGCACACATCATCATCATCAAGAAATCAATTTTTCTCTCTGAATCGAGGGAAGTGTTAGGTTTTTTTTGGAGAAGAGGGGAATATGCAACAAAGACTCAAGTTGCAGCAACAACAACAAGCACTCATGCAGCAAGCTTTGCTTCAGCAACAGCAGATGTACCATCACCCTGGCATGCTTGCCGCTGCCGCTATGACTCAGATGGAGCCTGTACCAAGCGGAAATCTACCTCCTGGTTTTGATGCTTCTGCATGCCGTAGTGTTTATGTAGGAAATATTCATGTAAAT
TARGET=143,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0979
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=AATCCACCATTTCTGAACCAAC
PRIMER_RIGHT_SEQUENCE=ACCTAACACTTCCCTCGATTCA
PRIMER_LEFT=114,22
PRIMER_RIGHT=233,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=0.0979
PRIMER_LEFT_1_PENALTY=0.095574
PRIMER_RIGHT_1_PENALTY=0.002310
PRIMER_LEFT_1_SEQUENCE=ACCAAATCCACCATTTCTGAAC
PRIMER_RIGHT_1_SEQUENCE=ACCTAACACTTCCCTCGATTCA
PRIMER_LEFT_1=110,22
PRIMER_RIGHT_1=233,22
PRIMER_LEFT_1_TM=60.096
PRIMER_RIGHT_1_TM=59.998
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=124
PRIMER_PAIR_PENALTY_2=0.1168
PRIMER_LEFT_2_PENALTY=0.095574
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=ACCAAATCCACCATTTCTGAAC
PRIMER_RIGHT_2_SEQUENCE=GTAGATTTCCGCTTGGTACAGG
PRIMER_LEFT_2=110,22
PRIMER_RIGHT_2=400,22
PRIMER_LEFT_2_TM=60.096
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.1168
PRIMER_LEFT_3_PENALTY=0.095574
PRIMER_RIGHT_3_PENALTY=0.021195
PRIMER_LEFT_3_SEQUENCE=ACCAAATCCACCATTTCTGAAC
PRIMER_RIGHT_3_SEQUENCE=AGGTAGATTTCCGCTTGGTACA
PRIMER_LEFT_3=110,22
PRIMER_RIGHT_3=402,22
PRIMER_LEFT_3_TM=60.096
PRIMER_RIGHT_3_TM=60.021
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.1297
PRIMER_LEFT_4_PENALTY=0.095574
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=AATCCACCATTTCTGAACCAAC
PRIMER_RIGHT_4_SEQUENCE=TTGTTGCATATTCCCCTCTTCT
PRIMER_LEFT_4=114,22
PRIMER_RIGHT_4=264,22
PRIMER_LEFT_4_TM=60.096
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_05870 Ps_contig_mira-and-tgicl-ass_franssen_40301
SEQUENCE=GGAGAGTGGGAGTGGATGGACATGGATTGAGAGGAGAGAGATTGACATTGGAAAATGGGGATTAAGGCGTGAGACTATACGAGAAGAGAGGAGAGTGCAAATTTCCCCTAACCAAATCCACCATTTCTGAACCAACCAACCAACCACCACCACCACTTCCATCTTCTCTCTCACGCACACATCATCATCATCAAGAAATCAATTTTTCTCTCTGAATCGAGGGAAGTGTTAGGTTTTTTTTGGAGAAGAGGGGAATATGCAACAAAGACTCAAGTTGCAGCAACAACAACAAGCACTCATGCAGCAAGCTTTGCTTCAGCAACAGCAGATGTACCATCACCCTGGCATGCTTGCCGCTGCCGCTATGACTCAGATGGAGCCTGTACCAAGCGGAAATCTACCTCCTGGTTTTGATGCTTCTGCATGCCGTAGTGTTTATGTAGGAAATATTCATGTAAAT
TARGET=180,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0445
PRIMER_LEFT_PENALTY=0.023267
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=ACTTCCATCTTCTCTCTCACGC
PRIMER_RIGHT_SEQUENCE=GTAGATTTCCGCTTGGTACAGG
PRIMER_LEFT=154,22
PRIMER_RIGHT=400,22
PRIMER_LEFT_TM=60.023
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=247
PRIMER_PAIR_PENALTY_1=0.0445
PRIMER_LEFT_1_PENALTY=0.023267
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=ACTTCCATCTTCTCTCTCACGC
PRIMER_RIGHT_1_SEQUENCE=GGTAGATTTCCGCTTGGTACAG
PRIMER_LEFT_1=154,22
PRIMER_RIGHT_1=401,22
PRIMER_LEFT_1_TM=60.023
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.0445
PRIMER_LEFT_2_PENALTY=0.023267
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=ACTTCCATCTTCTCTCTCACGC
PRIMER_RIGHT_2_SEQUENCE=AGGTAGATTTCCGCTTGGTACA
PRIMER_LEFT_2=154,22
PRIMER_RIGHT_2=402,22
PRIMER_LEFT_2_TM=60.023
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.0574
PRIMER_LEFT_3_PENALTY=0.023267
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=ACTTCCATCTTCTCTCTCACGC
PRIMER_RIGHT_3_SEQUENCE=TTGTTGCATATTCCCCTCTTCT
PRIMER_LEFT_3=154,22
PRIMER_RIGHT_3=264,22
PRIMER_LEFT_3_TM=60.023
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=111
PRIMER_PAIR_PENALTY_4=0.0574
PRIMER_LEFT_4_PENALTY=0.023267
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=ACTTCCATCTTCTCTCTCACGC
PRIMER_RIGHT_4_SEQUENCE=TCTTTGTTGCATATTCCCCTCT
PRIMER_LEFT_4=154,22
PRIMER_RIGHT_4=267,22
PRIMER_LEFT_4_TM=60.023
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=114
=
PRIMER_SEQUENCE_ID=SSR_05871 Ps_contig_mira-and-tgicl-ass_franssen_40301
SEQUENCE=GGAGAGTGGGAGTGGATGGACATGGATTGAGAGGAGAGAGATTGACATTGGAAAATGGGGATTAAGGCGTGAGACTATACGAGAAGAGAGGAGAGTGCAAATTTCCCCTAACCAAATCCACCATTTCTGAACCAACCAACCAACCACCACCACCACTTCCATCTTCTCTCTCACGCACACATCATCATCATCAAGAAATCAATTTTTCTCTCTGAATCGAGGGAAGTGTTAGGTTTTTTTTGGAGAAGAGGGGAATATGCAACAAAGACTCAAGTTGCAGCAACAACAACAAGCACTCATGCAGCAAGCTTTGCTTCAGCAACAGCAGATGTACCATCACCCTGGCATGCTTGCCGCTGCCGCTATGACTCAGATGGAGCCTGTACCAAGCGGAAATCTACCTCCTGGTTTTGATGCTTCTGCATGCCGTAGTGTTTATGTAGGAAATATTCATGTAAAT
TARGET=281,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0235
PRIMER_LEFT_PENALTY=0.002310
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=TGAATCGAGGGAAGTGTTAGGT
PRIMER_RIGHT_SEQUENCE=GGTAGATTTCCGCTTGGTACAG
PRIMER_LEFT=212,22
PRIMER_RIGHT=401,22
PRIMER_LEFT_TM=59.998
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=0.0235
PRIMER_LEFT_1_PENALTY=0.002310
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=TGAATCGAGGGAAGTGTTAGGT
PRIMER_RIGHT_1_SEQUENCE=GTAGATTTCCGCTTGGTACAGG
PRIMER_LEFT_1=212,22
PRIMER_RIGHT_1=400,22
PRIMER_LEFT_1_TM=59.998
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=189
PRIMER_PAIR_PENALTY_2=0.0235
PRIMER_LEFT_2_PENALTY=0.002310
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=TGAATCGAGGGAAGTGTTAGGT
PRIMER_RIGHT_2_SEQUENCE=AGGTAGATTTCCGCTTGGTACA
PRIMER_LEFT_2=212,22
PRIMER_RIGHT_2=402,22
PRIMER_LEFT_2_TM=59.998
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=5.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=191
PRIMER_PAIR_PENALTY_3=0.0445
PRIMER_LEFT_3_PENALTY=0.023267
PRIMER_RIGHT_3_PENALTY=0.021195
PRIMER_LEFT_3_SEQUENCE=ACTTCCATCTTCTCTCTCACGC
PRIMER_RIGHT_3_SEQUENCE=GTAGATTTCCGCTTGGTACAGG
PRIMER_LEFT_3=154,22
PRIMER_RIGHT_3=400,22
PRIMER_LEFT_3_TM=60.023
PRIMER_RIGHT_3_TM=60.021
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=247
PRIMER_PAIR_PENALTY_4=0.0445
PRIMER_LEFT_4_PENALTY=0.023267
PRIMER_RIGHT_4_PENALTY=0.021195
PRIMER_LEFT_4_SEQUENCE=ACTTCCATCTTCTCTCTCACGC
PRIMER_RIGHT_4_SEQUENCE=GGTAGATTTCCGCTTGGTACAG
PRIMER_LEFT_4=154,22
PRIMER_RIGHT_4=401,22
PRIMER_LEFT_4_TM=60.023
PRIMER_RIGHT_4_TM=60.021
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_05872 Ps_contig_mira-and-tgicl-ass_franssen_40304
SEQUENCE=CGTTTCAGAAACCAGAATATCTAGTTATAGATTTCGTCTCTTTTCTTAGTTTGACTCTTCCAAACTAAGACCAATAAGACTTCCCAATTAATATTTTTTTTTCTCATAATACTGACACGTCGACATCTGCAATAATTTCAATGTAATTACGTATGTCGGTATCGTGTCAGAGACACTGATACGAGGTGTTGAAAATATGATTATTTGGATGTAAATTGAGTATTACTAGGGTGAAGAGAGTGAATCAAGAGGGTCTCATGAGTGTGTGGAAGCCATTTTTGTGCAGCCAAGTTTGTGAAGAGGGTGTTCTTATTTGAGGTGGGAATCCTGATGATGCTGCAGCAGCAGATGATAAATAATGTGATCCTGGGTTGCCATTGTTGTTGTTGTTGCTGATGATTGTTTGTTGATAACGATAGATCGCTTCCAACTCGGCCTCCGTAGCTGCTGCTAGGAAAATGAAAATTTGGTGGTTGATGATTAGTATGTGCAAAACTGTGAAGCAYTTGTGGTTGATCTCGAAAGGGACCCGTATCGATGAATACCGTTTGGCGACGAAGATTGAAGATGAGTTTGACTTAGCATTCTCAGTGCTTCATTCTCTCCATGTAAATCTCTTCCATATGGTTCCATGTTGTTTGGTCTATTATATGGTTTTGGTAAAATATTCACAAGCTTAGGCTTGTTTATGTTCCCCCCATTCTGCCAATTAGATTGATCATGATTTGCAACATTTGATGTGTTATTCCCAAATGATTGAGTGTTATTCATGTGCTTTGAACTTGAATTTCCCAAACTTAAATCCAAATTGTGATCACTTCCACTACTTGCTGGCCGAC
TARGET=378,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05873 Ps_contig_mira-and-tgicl-ass_franssen_40305
SEQUENCE=TTATTTCGCAAATTCGAAATCGTTAACAGTTAACAGTATAGTTAGTTAGTTAGCACTTACAATGTCCCACGCGGCTTCATCGAGTGGTTTTCCACAATCAATAGACTCAAGCTTCGCTAGTTCAGGCTTTTTCTCAGTAACCTTAGCGGCAATGGCGCGTAGATAGCGTGCACGAACAGCGCCGGAAGCGGTAGCCCAATCAGCGCCTTTGTTACGCGTGAGAGCAGTTTTAGCGGCGGCGACGGCGACGTCGACGTCTTCCTTAGTAGCGGCAGGGATATCGCCGATGATTGGTTTTGGGGTGGCGGGATTGATGGACGGGAATCGTTGTAGNGGNCTTACNGTCGCGTTAANGAAC
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05874 Ps_contig_mira-and-tgicl-ass_franssen_40308
SEQUENCE=ACTAACAAAAAAAGAAAATTGATGAACACGAAAGAAAGAGAATAAGGGGTTGTTGTTTTTACAGACAAGGTCCAAACCACCACGAAAATGGCCAACATCTTGAAATCCAATTCAGGAAGAATCGCACTTCGTTCAATCACCCGGTCGTTGGCGGCGGCGCCAACTACAACTACCAACCACACTTCTTCACTTCCCTCTCGATTATCTTCCGTTCGATTTCTTCGCAGTGGTCGAGATCCTATCTCAAGGAATTATGAAACTATTCCCCCGGTTAATTGGGGGGTTCGAATTGTACCGGAGAAGAAAGCGTTTGTCATTGAGCGATTCGGAAAATACGTGAAGACTCTTCCATCTGGAATTCATTTCTTGATCCCGTTTGTGGATAGAATTGCGTACGTGCATTCGCTAAAGGAAGAAGCAATTAGTATTCCTGATCAGTCTGCTATCACTAAGGATAATGTCACCATTCTCATTGATGGTGTGCTTTATGTTAAGATTGTGGATCCTAAACTTGCTTCTTATGGGGTTGAGAATCCCATTTATGCTGTTATTCAGCTGGCTCAAACCACAATGCGTAGTGAACTTGGTAAGATTACTCTCGACAAGACCTTTGCGGAAAGGGATACGCTCAATGAAAATATTGTGGAGTCCATTAACGTGGCTGCAAAAAGTTGGGGTCTAGAATGCCTTCGATATGAAATAAGGGATATCTCTCCTCCACGTGGAGTGAAAGCAGCTATGGAGATGCAAGCAGAAGCAGAACGGAAAAAGAGAGCTCAAATTCTTGAGTCTGAAGGAGAAAGGCAGGCTCATATAAACATTGCTGAAGGAAAGAAGAGTTCAGTGATCTTGGCATCAGAAGCATCTATGATGGATCAAGCAAACAGAGCAAGAGGTGAGGCTGAAGCCATCATTGCCAAGGCAAGAGCAACAGCTGAAGGACTTTCTGTCGTGTCACAAACTCTAAAAGAAAATGGAGGACCAGAGGCAGCAAGCTTAAGGATAGCAGAGCAATATATTCACGCTTTCAGTAACATAGCCAAGGAGGGCACCACAATGTTGCTTCCTAGCTCTGCATCCAATCCTGCTAACATGATGGCTCAAGCCCTTACTATGTACAAAAGCTTAATTGGTAATGTTCCCAGTAATACACGCAGTGGGACCACACCTCCTTCAATAGCAGGACAAAAAGAAGGAAATTATTCATCTGATGAGGTTAAAGATGAGAGCTCAACAACCCTTACTACAACCAGAGGAATCCCAGATCATCCTGGAACATCTGGATTTTCACTTCAGAGTCCCCCAAATAGAGAGTAGTATTTGATTTTACTGAACTTGCCATTCTAATGAGTTAATTTGTTCTTTGATGCATGGATGTTATTGTGAAAATTGAGTTCAGAGTTCGGAGAAAGGGAAGTAGAAGAGTTTTTATTAAACTTCATTTTTTATCTCTAGTTGTTGTTGCATTTATCACAGGATACACATAGTTTATGACTAGACAATAGATATTGAATATCTCTTTCTTCCTAACTGGCAAATTTGGGGAGAAAGAAAGAAATGAAAAGAAGTATCATGTCTTAGTATTTTCTTCCATTG
TARGET=1546,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05875 Ps_contig_mira-and-tgicl-ass_franssen_40322
SEQUENCE=AAAANAACTCTTTGTTTCTCTCTCTATTTAGTGTCTCTGTCTTTAAACCAAATCTAGAGCAACCCATTTCGTTGTTTAAGCTTCTAGGGTTTCCTTCTTCTTCCATTTCGCTGAATGACTACACACCCTTTTCCACACTGACAAGACTGTACTCTCTCTCTTTCAAACAAGGCCTTTGCTTTCGACTCTATTATGGCTGGAGAAATAGCATGGATCAGCCACTATGATGATGATGGCTACTTGCAAAAGAAGACTGAGGACTTTGATTTGGATCTTAGTGAGGARGTTAATAAAGAAGCGAGTGCTGTTTCTGTTGACGTGATTTTGCCAGATGATTTA
TARGET=152,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05876 Ps_contig_mira-and-tgicl-ass_franssen_40322
SEQUENCE=AAAANAACTCTTTGTTTCTCTCTCTATTTAGTGTCTCTGTCTTTAAACCAAATCTAGAGCAACCCATTTCGTTGTTTAAGCTTCTAGGGTTTCCTTCTTCTTCCATTTCGCTGAATGACTACACACCCTTTTCCACACTGACAAGACTGTACTCTCTCTCTTTCAAACAAGGCCTTTGCTTTCGACTCTATTATGGCTGGAGAAATAGCATGGATCAGCCACTATGATGATGATGGCTACTTGCAAAAGAAGACTGAGGACTTTGATTTGGATCTTAGTGAGGARGTTAATAAAGAAGCGAGTGCTGTTTCTGTTGACGTGATTTTGCCAGATGATTTA
TARGET=224,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05877 Ps_contig_mira-and-tgicl-ass_franssen_40331
SEQUENCE=CGACAACACAAATTCATTCATTCATGTCTGTTTCTTCCGCTTCTTCTTCATCATCTTCTCAAAATCATAATTTTCTTCTCATTCTCTCTTTCATTTTTCACATCAACAAAAAAAACAACAAAAACGTTGTAATGGGTCTTTTACTTTCAAGGAGTTTGGCTTGCAAGTTCTTTTACCCTTTGCTTTTTTCTAAGGATGTTCATAATGATTTCTTTCCTCAACAATTGGAAAACATGATCAGTTTGGTCTCTCCTCGAACTGGAAGACATTTGCAGCGATATGAAAAAGGGTGTCGCCAAGTTGTGGGATGCATACCTTACAGATACAAAAGCAATGGAACCCAAGAAAATGAAATAGAAGTTCTTTTGATTAGTGCTCAAAAGGGTAGTGGAATGCAATTCCCAAAGGGTGGTTGGGAAAGTGATGAATCCATGGAACAAGCTGCTTTGAGAGAAACCATAGAAGAAGCTGGGGTTATTGGAAGTGTTGAAAGTAATTTGGGTAAATGGTATTATAAGAGCAAAAGGCAACCCATTATGCATGAAGGATACATGTTTCCTTTGCTTGTTAGCAAGGAGTTGGATAATTGGCCAGAAATGAACTTAAGAAAAAGAAAATGGTTGAGTGTGGATGAAGCAAAAGAAATATGCCCATATGCATGGATGAAGGAAGCTTTAGATGAATTGGTTAGTAGACAAACAACCAATTCAATCTAAGTTTTTTAGGTTATAAAATCATAGGCATTCTCTTCTCTCTTGCTTTTGGAATCTACAAAGTGGTAGAACGGGAGCACTTAAAAGACATTTGGACATAGAAAATGAAATCAAAAATCAAGAATTGGGAAATTGTGCAACACAAGAGTTTGTCAATTTGTTGTCCCTTTGGATTTGTTCATGTCTTCAAATTTGCATGTGAGTTGGCAAGATCAGCAGCAAGTTATGAGTTGTTAGAGTGTAGTTGAGATCTAACTATAGAAAATCTTTGTAGATGTACAAAAGGGTGGGTCAATTCATTCATTATTTCATTCAATTCATTCTTTGGCAATGTATGTAATAAGCTATAAGCTATGTGTAATACTACACTCTTCTAATTTGCTGTTGAAGACTATAATTGGTTTCTATTGCTATYNCACTTTGATTTG
TARGET=12,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05878 Ps_contig_mira-and-tgicl-ass_franssen_40333
SEQUENCE=CTTATTGATGTTTATTTTCGTTCATGTATTGAGGGTTCTTCACCTTTTACTTCCAGGTATGGAGGCTGTTGGCATTGTGACAGCTGTCGGTGCCGGCCGCACTGGTATACAGGTTGGAGATCTTGTAGGTTACGCTGGTCAACCAATGGGCTCATATGCCGAAGAGCAGATTCTTCCTGCAAACAAAGTAATTCCTATCCCTTCCTCCATTGAACCAGCTGTTGCAGCATCCGTCTTGCTGAAAGGCATGACAGCTCAATTTTTGCTCCGCCGTTGCTTCAAGGTTGAACCTGGTCACACAATTCTTGTCCATGCTGCAGCTGGAGGAGTTGGATCCCTGTTGTGCCAGTGGGCAAATGCACTTGGTGCAACTGTTATAGGAACTGTATCCAATAAGGAGAAAGCAGCTCAAGCCAAGGAGGATGGCTGTCATCATGTTATAATCTATAAAGAAGAGGATTTTGTGGCACGTGTCAATGAAATTACGTCAGGCGCTGGTGTTGAAGTCGTCTATGATTCTGTGGGAAAGGATACCTTTGAGGGATCTCTGGCATCCTTGAAGCTTAGAGGCTACATGGTCAGTTTCGGACAGTCTTCTGGTTCACCTGATCCAGTTCCATTGTCGTCTTTGGCTGCCAAAGCTCTGTTCTTAACAAGGCCTTCCTTGTTTCAATATGTTATCACTCGCGATGAGCTATTGGAGGCTGCTGGTGAGCTGTTTGCTAATGTTGCTTCAGGTGTATTGAAGATTAGAGTTAATCATACTTATCCACTGTCTGAAGCAGCAAAAGCACACGAAGACTTGGAGAATAGGAAAACCTCTGGATCTGTTGTGTTGATACCATGAGGCTATGTGTTGTTGTTGTTGGTATTAAAACATTTAAGTCCTTGTTGGGTACCTGTTGAAAGAAACTGTTTTATTTTGATAAATAAAAGGGACTATTTTGCTAAACATCAGTTGCTGTGCTTAAATAATTACCACCTTTCTGTTATCATAGTCATGATATTCAATAATTCAAACCTATGGCTTTGTTTTAGTTAAACC
TARGET=855,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4446
PRIMER_LEFT_PENALTY=0.129818
PRIMER_RIGHT_PENALTY=0.314775
PRIMER_LEFT_SEQUENCE=ATCCAGTTCCATTGTCGTCTTT
PRIMER_RIGHT_SEQUENCE=CAACAGGTACCCAACAAGGACT
PRIMER_LEFT=608,22
PRIMER_RIGHT=904,22
PRIMER_LEFT_TM=59.870
PRIMER_RIGHT_TM=60.315
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=297
PRIMER_PAIR_PENALTY_1=0.4558
PRIMER_LEFT_1_PENALTY=0.045831
PRIMER_RIGHT_1_PENALTY=0.410006
PRIMER_LEFT_1_SEQUENCE=GTGAGCTGTTTGCTAATGTTGC
PRIMER_RIGHT_1_SEQUENCE=AGCACAGCAACTGATGTTTAGC
PRIMER_LEFT_1=710,22
PRIMER_RIGHT_1=967,22
PRIMER_LEFT_1_TM=59.954
PRIMER_RIGHT_1_TM=59.590
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.4915
PRIMER_LEFT_2_PENALTY=0.081536
PRIMER_RIGHT_2_PENALTY=0.410006
PRIMER_LEFT_2_SEQUENCE=TGAGCTGTTTGCTAATGTTGCT
PRIMER_RIGHT_2_SEQUENCE=AGCACAGCAACTGATGTTTAGC
PRIMER_LEFT_2=711,22
PRIMER_RIGHT_2=967,22
PRIMER_LEFT_2_TM=60.082
PRIMER_RIGHT_2_TM=59.590
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.5021
PRIMER_LEFT_3_PENALTY=0.187326
PRIMER_RIGHT_3_PENALTY=0.314775
PRIMER_LEFT_3_SEQUENCE=TTGCTAATGTTGCTTCAGGTGT
PRIMER_RIGHT_3_SEQUENCE=CAACAGGTACCCAACAAGGACT
PRIMER_LEFT_3=719,22
PRIMER_RIGHT_3=904,22
PRIMER_LEFT_3_TM=59.813
PRIMER_RIGHT_3_TM=60.315
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=0.5021
PRIMER_LEFT_4_PENALTY=0.187326
PRIMER_RIGHT_4_PENALTY=0.314775
PRIMER_LEFT_4_SEQUENCE=TGGTGAGCTGTTTGCTAATGTT
PRIMER_RIGHT_4_SEQUENCE=CAACAGGTACCCAACAAGGACT
PRIMER_LEFT_4=708,22
PRIMER_RIGHT_4=904,22
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=60.315
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=197
=
PRIMER_SEQUENCE_ID=SSR_05879 Ps_contig_mira-and-tgicl-ass_franssen_40337
SEQUENCE=AATCAAGTTATGAGATAATCCAAATTCAACACCAACGTTTCTTCCAAGCTTCTCCCTCTCTCTCTGATCATCATCGCCCATCCCTTCCATTTTCGATTCTCAATCAAAGCTTCGCAGCTCCATCTTTCCAATCAAAGTTTAGGTAAGTGCATGCAGTAGTTTTGTTGATGAATGGCTTCCTCTGATGGTAGAGTTGCGTACTCTTCACTTTCTAAGTTAGAAAAGGGGAAACCCCACTTTGGTTATGATGATATGAATTTCAAGGGCGAG
TARGET=56,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2818
PRIMER_LEFT_PENALTY=0.261998
PRIMER_RIGHT_PENALTY=0.019805
PRIMER_LEFT_SEQUENCE=ATTCAACACCAACGTTTCTTCC
PRIMER_RIGHT_SEQUENCE=CATCATAACCAAAGTGGGGTTT
PRIMER_LEFT=23,22
PRIMER_RIGHT=249,22
PRIMER_LEFT_TM=60.262
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=227
PRIMER_PAIR_PENALTY_1=0.3576
PRIMER_LEFT_1_PENALTY=0.261998
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=ATTCAACACCAACGTTTCTTCC
PRIMER_RIGHT_1_SEQUENCE=TCTACCATCAGAGGAAGCCATT
PRIMER_LEFT_1=23,22
PRIMER_RIGHT_1=191,22
PRIMER_LEFT_1_TM=60.262
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.3576
PRIMER_LEFT_2_PENALTY=0.261998
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=ATTCAACACCAACGTTTCTTCC
PRIMER_RIGHT_2_SEQUENCE=CTACCATCAGAGGAAGCCATTC
PRIMER_LEFT_2=23,22
PRIMER_RIGHT_2=190,22
PRIMER_LEFT_2_TM=60.262
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=168
PRIMER_PAIR_PENALTY_3=0.4244
PRIMER_LEFT_3_PENALTY=0.404566
PRIMER_RIGHT_3_PENALTY=0.019805
PRIMER_LEFT_3_SEQUENCE=CAACGTTTCTTCCAAGCTTCTC
PRIMER_RIGHT_3_SEQUENCE=CATCATAACCAAAGTGGGGTTT
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=60.405
PRIMER_RIGHT_3_TM=59.980
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=0.5001
PRIMER_LEFT_4_PENALTY=0.404566
PRIMER_RIGHT_4_PENALTY=0.095574
PRIMER_LEFT_4_SEQUENCE=CAACGTTTCTTCCAAGCTTCTC
PRIMER_RIGHT_4_SEQUENCE=TCTACCATCAGAGGAAGCCATT
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=191,22
PRIMER_LEFT_4_TM=60.405
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_05880 Ps_contig_mira-and-tgicl-ass_franssen_40339
SEQUENCE=CAAGATAATAGATGAACAAGATTTACCAAAACTTCCATATCTCCAAAACATAATCTCTGAGGCACTTAGGTTACATCCACCAGCACCACCACCACCTCCTCCTGCACCACCTCCGTCACCACTGCCACCATACCCCCGTCGATCACTTGATGATGGCCCTCTACCTCCATGG
TARGET=84,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5996
PRIMER_LEFT_PENALTY=0.368190
PRIMER_RIGHT_PENALTY=1.231371
PRIMER_LEFT_SEQUENCE=TGAGGCACTTAGGTTACATCCA
PRIMER_RIGHT_SEQUENCE=AGGTAGAGGGCCATCATCAAGT
PRIMER_LEFT=57,22
PRIMER_RIGHT=165,22
PRIMER_LEFT_TM=59.632
PRIMER_RIGHT_TM=61.231
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=1.7104
PRIMER_LEFT_1_PENALTY=0.368190
PRIMER_RIGHT_1_PENALTY=1.342192
PRIMER_LEFT_1_SEQUENCE=TGAGGCACTTAGGTTACATCCA
PRIMER_RIGHT_1_SEQUENCE=GGTAGAGGGCCATCATCAAGT
PRIMER_LEFT_1=57,22
PRIMER_RIGHT_1=164,21
PRIMER_LEFT_1_TM=59.632
PRIMER_RIGHT_1_TM=60.342
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=108
PRIMER_PAIR_PENALTY_2=1.8034
PRIMER_LEFT_2_PENALTY=0.368190
PRIMER_RIGHT_2_PENALTY=1.435246
PRIMER_LEFT_2_SEQUENCE=TGAGGCACTTAGGTTACATCCA
PRIMER_RIGHT_2_SEQUENCE=GTAGAGGGCCATCATCAAGTG
PRIMER_LEFT_2=57,22
PRIMER_RIGHT_2=163,21
PRIMER_LEFT_2_TM=59.632
PRIMER_RIGHT_2_TM=59.565
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=107
PRIMER_PAIR_PENALTY_3=1.8160
PRIMER_LEFT_3_PENALTY=0.584629
PRIMER_RIGHT_3_PENALTY=1.231371
PRIMER_LEFT_3_SEQUENCE=AGGCACTTAGGTTACATCCACC
PRIMER_RIGHT_3_SEQUENCE=AGGTAGAGGGCCATCATCAAGT
PRIMER_LEFT_3=59,22
PRIMER_RIGHT_3=165,22
PRIMER_LEFT_3_TM=59.415
PRIMER_RIGHT_3_TM=61.231
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=1.8379
PRIMER_LEFT_4_PENALTY=0.368190
PRIMER_RIGHT_4_PENALTY=1.469712
PRIMER_LEFT_4_SEQUENCE=TGAGGCACTTAGGTTACATCCA
PRIMER_RIGHT_4_SEQUENCE=AGGTAGAGGGCCATCATCAAG
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=165,21
PRIMER_LEFT_4_TM=59.632
PRIMER_RIGHT_4_TM=60.470
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_05881 Ps_contig_mira-and-tgicl-ass_franssen_40344
SEQUENCE=CATTCATAAATCACTCCATTTTCAATTTCATTCTAATAACATTTATTTGCTTACATAATTTTCATTCATACTCATAAGGACACTTCATTGGAATTTTCTATTCCGATTGACTAATCTCCATAAAAAATAGAACACTATTTGGTTTTTACCAATCTTGTTTCTTAACTTAAGAAGTCCTAAAAAAATCTCAATATTCAATCACCAAGTATCTTTATTCACTTCAAAATACAAGCTTTTCTCAAACCACCACCATTCTTCTATTCTTGTGCCTTCATCTTAGAATCATTTCCATCATAATGAATCTGGAAGCACACAGTTGTGCACCGTCAGACTAACTACGGTAAGGTCGTTATTTTTCTTCTTCTTCTTCTTCTTCTGTTATTTAGCTTTTCTTTCTATACCATGTTCGTTCCCCTTGTTGACACTGATTAATACCTGTAGTAATTTGTTAGTAACTTGGTCTTCATAGTAATTTAATTTTAAATTTTAACGTTTAAAGGTAGGTTAGGTATTACTA
TARGET=356,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4039
PRIMER_LEFT_PENALTY=0.135980
PRIMER_RIGHT_PENALTY=0.267873
PRIMER_LEFT_SEQUENCE=GTGCACCGTCAGACTAACTACG
PRIMER_RIGHT_SEQUENCE=AATCAGTGTCAACAAGGGGAAC
PRIMER_LEFT=318,22
PRIMER_RIGHT=429,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.268
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=112
PRIMER_PAIR_PENALTY_1=0.5223
PRIMER_LEFT_1_PENALTY=0.254467
PRIMER_RIGHT_1_PENALTY=0.267873
PRIMER_LEFT_1_SEQUENCE=CTTTTCTCAAACCACCACCATT
PRIMER_RIGHT_1_SEQUENCE=AATCAGTGTCAACAAGGGGAAC
PRIMER_LEFT_1=232,22
PRIMER_RIGHT_1=429,22
PRIMER_LEFT_1_TM=60.254
PRIMER_RIGHT_1_TM=60.268
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.5292
PRIMER_LEFT_2_PENALTY=0.254467
PRIMER_RIGHT_2_PENALTY=0.274705
PRIMER_LEFT_2_SEQUENCE=CTTTTCTCAAACCACCACCATT
PRIMER_RIGHT_2_SEQUENCE=AGGGGAACGAACATGGTATAGA
PRIMER_LEFT_2=232,22
PRIMER_RIGHT_2=415,22
PRIMER_LEFT_2_TM=60.254
PRIMER_RIGHT_2_TM=59.725
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=184
PRIMER_PAIR_PENALTY_3=0.6625
PRIMER_LEFT_3_PENALTY=0.394672
PRIMER_RIGHT_3_PENALTY=0.267873
PRIMER_LEFT_3_SEQUENCE=CTCAAACCACCACCATTCTTCT
PRIMER_RIGHT_3_SEQUENCE=AATCAGTGTCAACAAGGGGAAC
PRIMER_LEFT_3=237,22
PRIMER_RIGHT_3=429,22
PRIMER_LEFT_3_TM=60.395
PRIMER_RIGHT_3_TM=60.268
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=193
PRIMER_PAIR_PENALTY_4=0.6651
PRIMER_LEFT_4_PENALTY=0.135980
PRIMER_RIGHT_4_PENALTY=0.529160
PRIMER_LEFT_4_SEQUENCE=GTGCACCGTCAGACTAACTACG
PRIMER_RIGHT_4_SEQUENCE=TAATCAGTGTCAACAAGGGGAA
PRIMER_LEFT_4=318,22
PRIMER_RIGHT_4=430,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=59.471
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=113
=
PRIMER_SEQUENCE_ID=SSR_05882 Ps_contig_mira-and-tgicl-ass_franssen_40345
SEQUENCE=GGAAAAATTGTCCATGAAGAAGAAGGGAATAGGATCCGAAGCAAGAGCTGAACGAATGGCTAATGCAGATACAGATATGCAGCTTTTGAATGAAGCCGAGAGAGGACGACATTTGCAGAAGCAGAAGAAACGCAGACTTCAAGGGCGTGAAGATGAAGTATTGTTGTTGTATTTTTATTTTATTTTATTTATTACTTTTGTCTCTCTTGTTTTTCTTTACTTTCCCGTCCCGTTTTCCTTT
TARGET=173,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7030
PRIMER_LEFT_PENALTY=0.054021
PRIMER_RIGHT_PENALTY=0.649008
PRIMER_LEFT_SEQUENCE=AGAAGAAACGCAGACTTCAAGG
PRIMER_RIGHT_SEQUENCE=GGGACGGGAAAGTAAAGAAAAA
PRIMER_LEFT=122,22
PRIMER_RIGHT=230,22
PRIMER_LEFT_TM=60.054
PRIMER_RIGHT_TM=60.649
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=109
PRIMER_PAIR_PENALTY_1=0.7089
PRIMER_LEFT_1_PENALTY=0.059875
PRIMER_RIGHT_1_PENALTY=0.649008
PRIMER_LEFT_1_SEQUENCE=ATTGTCCATGAAGAAGAAGGGA
PRIMER_RIGHT_1_SEQUENCE=GGGACGGGAAAGTAAAGAAAAA
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=230,22
PRIMER_LEFT_1_TM=59.940
PRIMER_RIGHT_1_TM=60.649
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=0.7089
PRIMER_LEFT_2_PENALTY=0.059875
PRIMER_RIGHT_2_PENALTY=0.649008
PRIMER_LEFT_2_SEQUENCE=TGTCCATGAAGAAGAAGGGAAT
PRIMER_RIGHT_2_SEQUENCE=GGGACGGGAAAGTAAAGAAAAA
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=230,22
PRIMER_LEFT_2_TM=59.940
PRIMER_RIGHT_2_TM=60.649
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.7174
PRIMER_LEFT_3_PENALTY=0.068420
PRIMER_RIGHT_3_PENALTY=0.649008
PRIMER_LEFT_3_SEQUENCE=AAGAAGGGAATAGGATCCGAAG
PRIMER_RIGHT_3_SEQUENCE=GGGACGGGAAAGTAAAGAAAAA
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=230,22
PRIMER_LEFT_3_TM=59.932
PRIMER_RIGHT_3_TM=60.649
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.8111
PRIMER_LEFT_4_PENALTY=0.162046
PRIMER_RIGHT_4_PENALTY=0.649008
PRIMER_LEFT_4_SEQUENCE=AGAAGCAGAAGAAACGCAGACT
PRIMER_RIGHT_4_SEQUENCE=GGGACGGGAAAGTAAAGAAAAA
PRIMER_LEFT_4=116,22
PRIMER_RIGHT_4=230,22
PRIMER_LEFT_4_TM=59.838
PRIMER_RIGHT_4_TM=60.649
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=115
=
PRIMER_SEQUENCE_ID=SSR_05883 Ps_contig_mira-and-tgicl-ass_franssen_40349
SEQUENCE=AACTTTAAAATNAACAAGTTTATTTATGATATAAAACAATAAGCCTAAAATACCCTCCACCTAATGCAATATAGGAGAATATCAAGCAAATAAACATAATAATAAAAATATAAAATGTYATATTCTGTAGTAATAAATTCCCCGAAATAACCCTCTAGAATATGTATGACTATGTCAAAGAAAACCAAAACTATGTAACCAATAAACAACAAACAAATAACTGTATCATACAAAGTCATCGAATCGGGTCAACAAACCCATCAACGATCCCAGTCACACTTGGTGGTCTGAAACCCACCGTCCACCGTCGCAGCACCGCCGCCAGAGTTCAAAGGAACATGCAACTCACAAGTAATCTTTGGCTTAATCGTCCTCGTCTTAAGCACTCCCAGCTTAAACCTAATCCTAAGATAAATCTTCACATCAATCTCATAAACCTTAGAATCCCTCTCTTTATTGAAATCCAAACTCTGATCACCGTCAAGAAGCACCACTTTCGAACCCTTAAACGAAGGGCTCAGCGTGTTAGTTGTTTTGTGTCCTTGATAAAAGGGACCAAGAAAAACAGAGTCAACTCTAGCATCATGGTACAAAGCCCTAGCTTCGATGTAGTCGTAGTAGATACCAAGTCTTTTGTTCGGGTTGCGGATTGTCATGTTGAGAGAGAGATCATAGTTGAGGTTGTTGTTGTTTGTGTAGTTGAATTGCGTGAGGGAAGCTTTGGTGACGTGGACTTTGACTACGTTGGGACGAACTATGAGCCAGAAGAGGAAGATGGCGATTCCGATGAGTATTATGACGGTGAGGATTAGTTTGAAGATGAGGTTGAAGAGACAACCGCAGCAGCAGTCGAAACCGCCGCCGCGGGATGGGCGGTGGTGGGATCTTGGTGGGGGGATGGAGGGGCCGTAGTAGGCTCCGTTGAGTTGTTGCTTCTCGTACATGGTTGTGGAGATAAAAGAGATTGGAAAAAATAAGATNTCCCCG
TARGET=660,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05884 Ps_contig_mira-and-tgicl-ass_franssen_40349
SEQUENCE=AACTTTAAAATNAACAAGTTTATTTATGATATAAAACAATAAGCCTAAAATACCCTCCACCTAATGCAATATAGGAGAATATCAAGCAAATAAACATAATAATAAAAATATAAAATGTYATATTCTGTAGTAATAAATTCCCCGAAATAACCCTCTAGAATATGTATGACTATGTCAAAGAAAACCAAAACTATGTAACCAATAAACAACAAACAAATAACTGTATCATACAAAGTCATCGAATCGGGTCAACAAACCCATCAACGATCCCAGTCACACTTGGTGGTCTGAAACCCACCGTCCACCGTCGCAGCACCGCCGCCAGAGTTCAAAGGAACATGCAACTCACAAGTAATCTTTGGCTTAATCGTCCTCGTCTTAAGCACTCCCAGCTTAAACCTAATCCTAAGATAAATCTTCACATCAATCTCATAAACCTTAGAATCCCTCTCTTTATTGAAATCCAAACTCTGATCACCGTCAAGAAGCACCACTTTCGAACCCTTAAACGAAGGGCTCAGCGTGTTAGTTGTTTTGTGTCCTTGATAAAAGGGACCAAGAAAAACAGAGTCAACTCTAGCATCATGGTACAAAGCCCTAGCTTCGATGTAGTCGTAGTAGATACCAAGTCTTTTGTTCGGGTTGCGGATTGTCATGTTGAGAGAGAGATCATAGTTGAGGTTGTTGTTGTTTGTGTAGTTGAATTGCGTGAGGGAAGCTTTGGTGACGTGGACTTTGACTACGTTGGGACGAACTATGAGCCAGAAGAGGAAGATGGCGATTCCGATGAGTATTATGACGGTGAGGATTAGTTTGAAGATGAGGTTGAAGAGACAACCGCAGCAGCAGTCGAAACCGCCGCCGCGGGATGGGCGGTGGTGGGATCTTGGTGGGGGGATGGAGGGGCCGTAGTAGGCTCCGTTGAGTTGTTGCTTCTCGTACATGGTTGTGGAGATAAAAGAGATTGGAAAAAATAAGATNTCCCCG
TARGET=681,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05885 Ps_contig_mira-and-tgicl-ass_franssen_40358
SEQUENCE=CTAACTACATTTCTTNTATCNCTATCATTCTCTTTCTACTACACTATGGCTGAGGAGGCATGGTAGCCACGGCATCTACTACCCCAAAAAAAGAATGAAGATAACGATCCTAAAACCAAACCTATAAATGCTGGTGGCTATGATGGTGGAGATGATGACTCTGACGACATAACACCTAGCGGTGGCGGTTACGGAGATGGCTATGATGATCCTGCTGTCCAAGCAGCCATTGGTGGTGGTTTTGGTGACTCTGATACCAAACCTACAACCGGTGGTGGCTACAATGGTGGCATTGATGGCTCTGATGATGGCTATGACGGAGGCTATGGAGATGGCTATGATGACCCTGCAATCCAAGCAGCTATTGGTGGTGGTTCTGGTGACTCTGACACCAAACCTACAACCGGTGGTGGCTACAATGGTGGCAATGATGAGTCTGATGATGGCTATGATGGCGGCTACGGAGATGGTTATGATGACCCAGCAATCCAAGCATCTATTGGTGGTGGTTATGATGGCTCGGACACCAAACCTACAATTGGTGGTGGAGTTGATGACTCTGACGACATAACACCTAGCGGTGGTGGCTACGGAGATGGCTTCGATGACCCTGCAATACAAACAACTACTGGTGGCGGCTATGGTGGTCCTGAGAAAAAGTAAGCAAAGGAGGAAGATGAAGAAAGAAGCTCGTGAAAAGAAGAAACACCATTTCTTTGGGTGAAAGATGTTTTTATATTATATTATAATAATAAGCATACAAGCATTATGGAAATATTCATTCTGCATAGCTTTTACCCTATAATTAATTATGCTTTTGGGCTGTTTATGTGTATTTATTCGTATTGAGTTCATCTAATCGCAGTTTAGTTTGAGTGGTTTGGAGAATGTAATTTCTAGCTTGGTGTCGTATAGATGATATATGCATGGATCTATGTTGTGTCTACCGATAAGTACACAATATACTATTGTAAAAGAATCTGATATGATTTTAAAAAAATTACTTGTTCTAG
TARGET=734,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9448
PRIMER_LEFT_PENALTY=0.132857
PRIMER_RIGHT_PENALTY=0.811989
PRIMER_LEFT_SEQUENCE=GATGACCCTGCAATACAAACAA
PRIMER_RIGHT_SEQUENCE=TACACATAAACAGCCCAAAAGC
PRIMER_LEFT=603,22
PRIMER_RIGHT=834,22
PRIMER_LEFT_TM=59.867
PRIMER_RIGHT_TM=59.188
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.9707
PRIMER_LEFT_1_PENALTY=0.158723
PRIMER_RIGHT_1_PENALTY=0.811989
PRIMER_LEFT_1_SEQUENCE=TGGTGGAGTTGATGACTCTGAC
PRIMER_RIGHT_1_SEQUENCE=TACACATAAACAGCCCAAAAGC
PRIMER_LEFT_1=542,22
PRIMER_RIGHT_1=834,22
PRIMER_LEFT_1_TM=60.159
PRIMER_RIGHT_1_TM=59.188
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.9707
PRIMER_LEFT_2_PENALTY=0.158723
PRIMER_RIGHT_2_PENALTY=0.811989
PRIMER_LEFT_2_SEQUENCE=GTGGTGGAGTTGATGACTCTGA
PRIMER_RIGHT_2_SEQUENCE=TACACATAAACAGCCCAAAAGC
PRIMER_LEFT_2=541,22
PRIMER_RIGHT_2=834,22
PRIMER_LEFT_2_TM=60.159
PRIMER_RIGHT_2_TM=59.188
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=294
PRIMER_PAIR_PENALTY_3=0.9766
PRIMER_LEFT_3_PENALTY=0.164639
PRIMER_RIGHT_3_PENALTY=0.811989
PRIMER_LEFT_3_SEQUENCE=GCAAAGGAGGAAGATGAAGAAA
PRIMER_RIGHT_3_SEQUENCE=TACACATAAACAGCCCAAAAGC
PRIMER_LEFT_3=663,22
PRIMER_RIGHT_3=834,22
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=59.188
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=1.0665
PRIMER_LEFT_4_PENALTY=0.254467
PRIMER_RIGHT_4_PENALTY=0.811989
PRIMER_LEFT_4_SEQUENCE=ACACCATTTCTTTGGGTGAAAG
PRIMER_RIGHT_4_SEQUENCE=TACACATAAACAGCCCAAAAGC
PRIMER_LEFT_4=705,22
PRIMER_RIGHT_4=834,22
PRIMER_LEFT_4_TM=60.254
PRIMER_RIGHT_4_TM=59.188
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=130
=
PRIMER_SEQUENCE_ID=SSR_05886 Ps_contig_mira-and-tgicl-ass_franssen_40369
SEQUENCE=CCCAAATCAAATATACACATACCAAGATCTTTGAAACTCTAAAACTTTCTATATTATCATCCTGTCTTCAAAGATCTTCTCCATGGATCTATGGTTTCCATGGTACGCACCACAAAGATATTAACAAAAGGTATTTACAAATTCAATAAATGGTGGTCAAGCTTCTGAAATACTAAAATCTATGTTGCACCATTTCATAAAAGTGAAAGTTGGAGAATTTGACAAGCATAAAAGTGCAGGTTGAAGAATTTGACAACTATGCTGCTGTCGTTGAAAGACTCCAAGACCGTTTCAGATCAGATCATGCGAGTAGAAAGTCCTTATTCAGCATTTTGAGTCTGATCCTGATTGATGAATTCCAAAGCATCACTCTCGGATATAACATTAACACGGCTGACCCTGTTTTTATGTGTCACTCCATATATCTTGTCAGCAACTTTAACTAGCTCTGGGCGGAAAGTTGTTGTGATAAATTGAGTGTTTGCTATGTCTGCCAGGCGCCGGATCATATTTCCAACAGCAGTTCTGTACTGAGGATCCAATGCTGCATCAATCTCATCGAATAGGTAAAAGGGAGCCGGATCACATCTCTGTATGGCAAAGATGAGTGTAAGTGCGACAACAGTTTTTNTACCCCCCGACCAACTGCTTCATAGACTGTGTCTCCCCTTGTCCAGTAAAAGAGACCTTTACTTTCACCCCAATGTACTTTTCTACTCTCCCCTCTGGATTTGCTTCCCGAGGACCATCCTCGTCTTGCTCATCATCATCATCATGATCACCATCCTTCTTCTTCATCATAACCAGATAACCACGGCCTCCCTGTACAAGTTCAGAGAATACTTCTCGGAAATGCCTAGCAACACCTTTGAAAGTGCGTTCTATTGATTCGTCCTTCCGCTGATCTAGCACTGTTATTAGCTCTTTGATTTTCTCATCTCCTGCGTCAAGTTCAGCCTGTCTTTTCTGAAGTTCTTCTCGCTGTTCTGTAAAATTTATATACTGATCAAGTGCTTTCTTGTTGACATGACTAAACTTTTGCAACTGATCATTGCAACTGTGCAGCTTTTTTTGCAACTCTTTAACATTCTTCCGTTTGTACATTTCAAAAGCATCTGATGTCAATGGGCCCAGTTCCCTTATTTTTTTAGTATATTCTTCTTCTTTAGCAGAATTTGTATTTTTTCTACTCAGCAATTGCTCTAATTCTTTAGCTTCTTCCTGAAGTTGCCTTTCATACTCGTCCTCCAAAGACTTCAATTTATTCATTTCATCTTTGATCTTCGTTATTTGCTTGGTTCGTTTATTTATACTATCAGAAACACTTTTAAGTTGAATTGATGCATCATCAACCGATGTTTTAGCATCATTAAGTTCCCGCTTCTTCAACTCAGCATCAACAACCAAAGAGTCATCATCAACAGATGATATCACAGCCTCCAGCTCTTGTTTTCTTTTCCTGAGATTAGTGGTTAGATTAGTTTCCAACTCTGCGTTGATACCACT
TARGET=761,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0982
PRIMER_LEFT_PENALTY=0.064105
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=CGACCAACTGCTTCATAGACTG
PRIMER_RIGHT_SEQUENCE=CGGAAGGACGAATCAATAGAAC
PRIMER_LEFT=638,22
PRIMER_RIGHT=899,22
PRIMER_LEFT_TM=59.936
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=0.0982
PRIMER_LEFT_1_PENALTY=0.064105
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=CGACCAACTGCTTCATAGACTG
PRIMER_RIGHT_1_SEQUENCE=GGAAGGACGAATCAATAGAACG
PRIMER_LEFT_1=638,22
PRIMER_RIGHT_1=898,22
PRIMER_LEFT_1_TM=59.936
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=261
PRIMER_PAIR_PENALTY_2=0.1033
PRIMER_LEFT_2_PENALTY=0.064105
PRIMER_RIGHT_2_PENALTY=0.039178
PRIMER_LEFT_2_SEQUENCE=CGACCAACTGCTTCATAGACTG
PRIMER_RIGHT_2_SEQUENCE=TAACAGTGCTAGATCAGCGGAA
PRIMER_LEFT_2=638,22
PRIMER_RIGHT_2=916,22
PRIMER_LEFT_2_TM=59.936
PRIMER_RIGHT_2_TM=60.039
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=10.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.1366
PRIMER_LEFT_3_PENALTY=0.102490
PRIMER_RIGHT_3_PENALTY=0.034102
PRIMER_LEFT_3_SEQUENCE=TCCCCTTGTCCAGTAAAAGAGA
PRIMER_RIGHT_3_SEQUENCE=CGGAAGGACGAATCAATAGAAC
PRIMER_LEFT_3=664,22
PRIMER_RIGHT_3=899,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=59.966
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.1366
PRIMER_LEFT_4_PENALTY=0.102490
PRIMER_RIGHT_4_PENALTY=0.034102
PRIMER_LEFT_4_SEQUENCE=TCTCCCCTTGTCCAGTAAAAGA
PRIMER_RIGHT_4_SEQUENCE=CGGAAGGACGAATCAATAGAAC
PRIMER_LEFT_4=662,22
PRIMER_RIGHT_4=899,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=59.966
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_05887 Ps_contig_mira-and-tgicl-ass_franssen_40374
SEQUENCE=ACAATACCCACTTCCTTTTAGTTAGTTTTTTAAACTTACTAATTANTCAACCTTCACATACAGCTATTTATTCACATTTTTTACCTAAAAAAGTAAAACTAGCCAAACCAGATCTCTGCATATAATAATGAACTCTTAACCTTCATATCGCCAATGACTACTAAGTAGAAAGGAAATTGAGTACATTTTTTACCAGGTATTTCAAGCTAATACAGGGAATCCAGATTCTCCGGAAGATGAAGGTTGTAATGACCGAATGCGGATGTCATATTTTAAACTCCTGGCCGCTTTGGAGCTAGGGGATAATTCTGCTCCGTCTGACACGAGTGGTAAGGAAGAAGTGAAACCATCGTATGCATTTCCAATATGGTTGCCACATTTTCGACAGAGAAGCTTAGTTCTTCGACGGAATAAGCCCCATGAATGCTTATGAAAATGAGGCACACACTGTATCTCATCAACCTGGGTAAATCTGTTATCATCAATATTGAAGAATGAGATGATACCTCGCTTTATGGACTTCCCGTATTTTGATCCGATGGATGTGGTATTCCGGTTTGAGGAGGATAGGTTCAGCTCATATCCACAAGAGCCACAGCTATAACGAACATCCCTCTGTGAAGCGGAAGAATAAGAAGAGAATGTTCGGTTAAAGTGGACATCTCCGTTGGAGAAAGATTTATCCATTGAATAATATTATCCGGTGACGGTGTGCGACGGTGGAGGTGGTGGCGGAAGTGGCTGATTGCGGAAGAGCCACCTTGGCGGCAAAGAGCGCGTGAGAGAGAGAGATGAGATATAGGAAGTTGGAGGCGTTGAGAGGACAGCGTGTCCGGATGAATCGTAACCACGTGTTTGACTGAATCCAGAACAAGATCGAAACGACGATGTTTTAGAAGTTACTGTACTTGTTCATAGAAGAGGCTTCGGTTTCGGTTTTAGTGACAGCAAAGAAAGTATCCCCCGCG
TARGET=781,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2468
PRIMER_LEFT_PENALTY=0.037486
PRIMER_RIGHT_PENALTY=0.209271
PRIMER_LEFT_SEQUENCE=CACAAGAGCCACAGCTATAACG
PRIMER_RIGHT_SEQUENCE=CAAACACGTGGTTACGATTCAT
PRIMER_LEFT=584,22
PRIMER_RIGHT=857,22
PRIMER_LEFT_TM=59.963
PRIMER_RIGHT_TM=59.791
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.7000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.3048
PRIMER_LEFT_1_PENALTY=0.095538
PRIMER_RIGHT_1_PENALTY=0.209271
PRIMER_LEFT_1_SEQUENCE=TCCACAAGAGCCACAGCTATAA
PRIMER_RIGHT_1_SEQUENCE=CAAACACGTGGTTACGATTCAT
PRIMER_LEFT_1=582,22
PRIMER_RIGHT_1=857,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=59.791
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.3987
PRIMER_LEFT_2_PENALTY=0.037486
PRIMER_RIGHT_2_PENALTY=0.361206
PRIMER_LEFT_2_SEQUENCE=CACAAGAGCCACAGCTATAACG
PRIMER_RIGHT_2_SEQUENCE=GTTCTGGATTCAGTCAAACACG
PRIMER_LEFT_2=584,22
PRIMER_RIGHT_2=871,22
PRIMER_LEFT_2_TM=59.963
PRIMER_RIGHT_2_TM=59.639
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.4520
PRIMER_LEFT_3_PENALTY=0.242742
PRIMER_RIGHT_3_PENALTY=0.209271
PRIMER_LEFT_3_SEQUENCE=CTCATATCCACAAGAGCCACAG
PRIMER_RIGHT_3_SEQUENCE=CAAACACGTGGTTACGATTCAT
PRIMER_LEFT_3=576,22
PRIMER_RIGHT_3=857,22
PRIMER_LEFT_3_TM=59.757
PRIMER_RIGHT_3_TM=59.791
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=282
PRIMER_PAIR_PENALTY_4=0.4537
PRIMER_LEFT_4_PENALTY=0.418705
PRIMER_RIGHT_4_PENALTY=0.035021
PRIMER_LEFT_4_SEQUENCE=GACATCTCCGTTGGAGAAAGAT
PRIMER_RIGHT_4_SEQUENCE=GTCACTAAAACCGAAACCGAAG
PRIMER_LEFT_4=657,22
PRIMER_RIGHT_4=945,22
PRIMER_LEFT_4_TM=59.581
PRIMER_RIGHT_4_TM=60.035
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_05888 Ps_contig_mira-and-tgicl-ass_franssen_40385
SEQUENCE=ACATCCCAAAAAATCGGGGATGTTCTTTTAATTTAAACAAAAGAAATGAATATGATAGATTAGATTGAGATAAGTGCCACCGGAGAAGAATGATTCGGCGGTTATGGTGTGTGCAGTGTCAGAACGCGTCGTTCTCTTCCACTTCCACCACCACCAACAAGAAGAAGCCCGTTGTTCTCTTGGGAGCACCTCAGGTCTCCACGATAGTCCTTGACGCACTTCTCACCGCTTCTGCTTCTCCACATTCTTCCTTTGAGGTTGCGGCTATTGTGACTCAGCCAGCCGCTAGAAGGGACAGAGGGAAAAAGCTTTCGCTCTCTCCCTTGGCCAACTATGCTCTTGACAGAGGCTTCTCTCAGAATCTTATTTTCACTCCTCAACGTGCCGGAGACGATACTTTCTTGTCCGATTTAAAAGCTTTGCAGCCGCAGCTATGCATTACGGCAGCTTATGGAAATATATTACCTACCAAGTTTCTAGATATCCCTCCTTTCGGAACAGTTAACATTCACCCTAGCCTTTTGCCAATGTATCGTGGTGCTGCTCCGGTCCAAAGGGCATTGCAGGATGGTGTTAAAGAAACTGGAGTGTCGTTAGCATTCACTGTTCGTGCACTCGATGCTGGACCTATCATTGCTACTGAAGCAGTTCAAGTTGATCATTATATAAAGGCACCTGATTTACTTGAACTCCTGTTTCACAAAGGGTCTGAACTTCTGATTCGGGAACTTCCGTCTATATTTGATGGATCAGCAAGAGTGAAGGCACAACCCCAAGATGATTCTAAGGCTACATTGGCACCAAAGATAGGTCCAGACGAATCATGGCTATTCTTTGATCAAGAAGCATCTGTTCTCCATAATAAGGTTCGTGCATTTTCAGGGTGGCCAGGATCTAGAGCGCAGATTCTAGTAGTTGACAAAAATGGTGAAAAGAAAACTTTGGAGATTAAAGTCATAACCACACGAGTTTGTACCCATGAAAGTGTGCAGTTCAATGAAGCAGATGACATTACATTTGTCAATGATGCATTGGTGTTTCCATGTGGAAGGGGCACCCCACTTGAGGTATTGGAGCTTCAGCTTCCCGGAAAAAAGGTGGTTAATGCAGCTGCTTTCTGGAATGGCTTGCGGGGACAGAAGCTGAAGAAAACATGAGCAGCAGTGGTTTATCGAACAGAAAGCCCTATTTAAAACTATCATCTTTCATCCGGCTGTTTGGGCAATTGATTCTTTCTTTTTTATTTTGATTTGATTCAAGATTTTCAATTTCTTCAATCATTTACATGATTTGTAGATAACTATCATATCAGTATTTTTATTTTAACACATACTATTTTAACCTTGAAAATAGAAAACTAGATGTATTGGAAGTCAAAGTTTTGGTCTTGTRCATCAGAAAGTCATTATAT
TARGET=145,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05889 Ps_contig_mira-and-tgicl-ass_franssen_40394
SEQUENCE=AGCTCATAATTTATATCTATANNTCCTTTATCTAACTAGTCAAATAAAGGAACAAAAGTAGTAGCTGAATAAAATTAAAATAAATAAAATATTTACATATATCTAAGAAATTTAAAAATCTCATTAGATAGATTAATTAATTAAAAGGAAATTTTCCTTTTCCTTTTTTCAATTTTCAATTTTCTTTTCATGATTTCTATTGTGCCGGCAAGGTTCCGTGCCGCCACGAGTCAGAAAAATCGTTCGAGTCATAATACACATCTGAATGGTTAAAAATCCCATCAAACGAGTCATAACCAAAACTTCCAAATCCGTTTCCTAAATCAAACGAGTCATAACTCGGGATCTGGTTCTCAAACTCCCATAACTCACCGATCCCGGACGAGTCAGACAAAACTCGAGCCAAATCAGTTTCATCTTTTTTCTCTTCATGAACAACTTCATGAACCGAAGGATATCCCGGTGGTGGAAGCCCGAGTTCGTCGTCGGAAGCTTCCAGAAGATACCCGATCTGAGCCTGAGACTCGCCGGAATCTGACATCACACGAACGGAAGTAGAAGAAGACGCAGAGATCTCCTCCTGCAAGCTCTTTATAACAGAATCAAGCTCTTGCATCGAAGGAGCTGGATCGGAATTGTTCAAGAACTCGAGTAAATCGTCTCTGAGTTTCTTCGCCTCGGACGATTCAATCTCTGACTCGGACGAGTCATTACGTAACCGCTTCTTGCAACTGTTATCATCAGCCATTTTTTTATTTATAAAAATTAAAAAGAATTAAAAAAAAAGACAAAGAGCAAAACGGTGGCGTTTAATAGGCTTGTGAGAGTTTGAACAAGAGTTGACACGGTCCCCGA
TARGET=132,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05890 Ps_contig_mira-and-tgicl-ass_franssen_40405
SEQUENCE=GAACATGTTGGATAGTGCTTATAATGAACAACAACAACATCATCGTCATCAGCAACAACAACATTCTAAGACGCAACACCAACAACAACATCAAGTTCAAACTCGGTATTCTTCGACGACTCCATCGAGTTACTATGAGCAGAATTGTGAAACAAATGAGATTGTGTTTGGTGCAGTTCAAGAACATGATGGAAGACGTGAAGCTATGGTGATTAAGGCGGTTGATTATGGTGATCCAAAATTCAGTCACCATAATATTCGTCCTAGATTAAATTATGTAGGTTACTCTAAAAGTTATTGAAGATTTCATAGTATCAAATTGCATGGATATTGATGCAATA
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05891 Ps_contig_mira-and-tgicl-ass_franssen_40409
SEQUENCE=GGTTAATAAACTTATGTCTATTTCCAGTAATTTAATAAATCGCATAATAAACAAANNNNTANGTTAAAAACNAAAGTTAACTAATACCAAAATTACTAAAAATTCAAAATTACAAAATTCCTAATTCATCATCATGAATAAATGGTGTAAAGAAGCTATCAAAATTAAACTTCCACCACCATCTCAACAACACATATCAACAAACGGTTTACAAAAACCGGTTTAATCAAGAAAACCGGTTTGCCGCAGAACCGCGTTTCGCACCTGACGGAGATCTCTCCCTTTGAGAGTTCTCCCAACTCCTTCCAAAACGGAAAAGGTAGTTCTAGGCGAAACTCCAGAGCCTCTCGCCACAAGTTCATGCGGCGGCAACAGCTCCTCGCCGTCGTCGTCGTCATCGTCCAACACCGCAAGTGCATCAACGTTCCGCCGTCTCGAGAATTGACTCGGTGGCAGAACCGGAACCTTCACTGGAGCCGATTGTTGGAACTTTCTAGAAGGCATAGACTGAACCAATTGCTCGGAGTTATAACCGGAAGAAGGTCTAGGTCTCGGAAACGAAGGAATCATTCTAGAAGACGAAACGGGAGATTTACCACGAAACACCGGTGAATCACGGTTGTCTGGACCTGATAGAACAGCAAGGATACCGGAGTCTTGAGATAGATCGAAACTCCGGTGACGAGTTAACTCAGTCGGTTGAGGCGCTGGACGTTGATTTTGATTTTCAGATTCGGAGCTATCAGATGACCAAAACAGTTCAGCTTCGTTGAGCTCATCGCCGGCGGAGGCGGTGACAGTGGAAGGAGGAGAGAAATTGAAAGCGCCGAGTAATCGGTCGGAGTTGGAAGGAAATCGGCGGTGAAAGGAATGTGTGGGGTCCATTTGGTTGGTGAATGAAAAGTTCTGGAGTGAAGAAGAATTGTGAGAAGGGAATATGAAATAAGAAAATGAGTAATGTCCCGAC
TARGET=384,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2731
PRIMER_LEFT_PENALTY=0.143960
PRIMER_RIGHT_PENALTY=0.129103
PRIMER_LEFT_SEQUENCE=TGAGAGTTCTCCCAACTCCTTC
PRIMER_RIGHT_SEQUENCE=TAGAAAGTTCCAACAATCGGCT
PRIMER_LEFT=284,22
PRIMER_RIGHT=495,22
PRIMER_LEFT_TM=59.856
PRIMER_RIGHT_TM=60.129
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=11.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.2731
PRIMER_LEFT_1_PENALTY=0.143960
PRIMER_RIGHT_1_PENALTY=0.129103
PRIMER_LEFT_1_SEQUENCE=TGAGAGTTCTCCCAACTCCTTC
PRIMER_RIGHT_1_SEQUENCE=CTAGAAAGTTCCAACAATCGGC
PRIMER_LEFT_1=284,22
PRIMER_RIGHT_1=496,22
PRIMER_LEFT_1_TM=59.856
PRIMER_RIGHT_1_TM=60.129
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=11.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=0.2738
PRIMER_LEFT_2_PENALTY=0.143960
PRIMER_RIGHT_2_PENALTY=0.129846
PRIMER_LEFT_2_SEQUENCE=TGAGAGTTCTCCCAACTCCTTC
PRIMER_RIGHT_2_SEQUENCE=GAGACCTAGACCTTCTTCCGGT
PRIMER_LEFT_2=284,22
PRIMER_RIGHT_2=552,22
PRIMER_LEFT_2_TM=59.856
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=11.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=269
PRIMER_PAIR_PENALTY_3=0.3753
PRIMER_LEFT_3_PENALTY=0.143960
PRIMER_RIGHT_3_PENALTY=0.231323
PRIMER_LEFT_3_SEQUENCE=TGAGAGTTCTCCCAACTCCTTC
PRIMER_RIGHT_3_SEQUENCE=CGAGCAATTGGTTCAGTCTATG
PRIMER_LEFT_3=284,22
PRIMER_RIGHT_3=522,22
PRIMER_LEFT_3_TM=59.856
PRIMER_RIGHT_3_TM=59.769
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=239
PRIMER_PAIR_PENALTY_4=0.4854
PRIMER_LEFT_4_PENALTY=0.143960
PRIMER_RIGHT_4_PENALTY=0.341461
PRIMER_LEFT_4_SEQUENCE=TGAGAGTTCTCCCAACTCCTTC
PRIMER_RIGHT_4_SEQUENCE=AGACCTAGACCTTCTTCCGGTT
PRIMER_LEFT_4=284,22
PRIMER_RIGHT_4=551,22
PRIMER_LEFT_4_TM=59.856
PRIMER_RIGHT_4_TM=59.659
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_05892 Ps_contig_mira-and-tgicl-ass_franssen_40416
SEQUENCE=AGAATACAAAAGAAGTAGAAGGAGAGAGAGAACGCAAAAAAAAGGGAGAAGATCAGAGAGAAGTAAACCGTGGGAAGCATACCTGAAGATCGCACCGAGGCTTGGAAATCGGCGAATCCGGAAGCGTTATTGTCACCTTCGTCCGTAACACACACGGTAGCCATAACACAACAAAGGTACGTACTAATGTGATTTCATGTTTTAAATCTAGTGCAACATGACAGTTTGAAGCTGCGAGTTAATCTCGCCATAGCTCGCCGCGAGCGAATAGAAATCCATCGAGTCGTTTTGTTGTTGATTTGTGCGTGTTGATCGTTGAAACGNGTGTGTGGAGTAAGCTTGTATTTTGGTTTGGTCTTCTGTCTTAGAATT
TARGET=22,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05893 Ps_contig_mira-and-tgicl-ass_franssen_40423
SEQUENCE=CATGAACCTCGGCATTTTTTAGNTTTGCATCATCAGCATGGTTCAGTGCCTGGTCCTTAGCATCACACATCATGGTGAGTTCTTGCTTAATCTGCTGAAGCTCCTCAGTGGTGGAAGCAAGAGCAGCCACATCCAAGGCATGCTGGTTCCTCACACTTTCAAGCTCTTTCTGCCATTCCTCTTCCTTTTTCTTCACAGTTTCAATTCCAGCCTGCTCCAATTCAACAGCTCGGAATTTTTCTATCTCAGATTCCTCCTTAGCCTGCTTTTGTTCCACCAATGCCTCCCTGAGTTTCTCATTTGCTTCCTCCGCTACTCTATGTGCTTCTTTCAACTCATCAGTAATTTTTTCCTTCTCTTTCTCAGCCTGAAGGATCTGTTCCTTTGCTTTCTTTAGATCTTCTTGAGATACATTCAACTGGTTCTGCAATTCTGAACCCTTTGCAGCTCTTGGTGCTTGTTTATCCGGTGGCGTGGAGCTAGCTTTTGCTGATTTCCGCTCAACAGGGGGCTTAGAAATCACAGATCGCGGCGACCTTTCCACAGAAACACGTGAATTTTGCAATGGAGGCGGCGATTCAGTTTCTGATTTGCTCCCTACTTTACTAACTTTACTGACTCTCGGTGTTGCTTGAGCTGCACCTTTGTTGGAATTTTCAGATAGTTTTGATCTAGTAGTACTAGTAGTAGATTTTGAAGCCATGGAAGCAGAGTAAGAAGAAAAAGAAAAACGGTTACTGAATTAGGAATGATAGAGTTGTTGTTGTTGTTATGACATTGTTAGTTAAGAAAGAAGAAGAAAATTCAGGATCAAAGTAATTTGGATGATTTTTGTGTGAAATTATTGGAAAGAGAGGGAATATAATTTACAGAGAAGAAACAAGAAAATTGAATGAGTTGGTTTTCATGTGTCTCCAGAGAGGCCACAATACACGCCACACTCTCTTTCTATTTTTTTCCTTTAACCGTTTTCTTCTATTCTCTCACTTATTATCTG
TARGET=757,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5411
PRIMER_LEFT_PENALTY=0.392624
PRIMER_RIGHT_PENALTY=0.148509
PRIMER_LEFT_SEQUENCE=GCCATGGAAGCAGAGTAAGAAG
PRIMER_RIGHT_SEQUENCE=CTCTGGAGACACATGAAAACCA
PRIMER_LEFT=698,22
PRIMER_RIGHT=919,22
PRIMER_LEFT_TM=60.393
PRIMER_RIGHT_TM=60.149
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.5411
PRIMER_LEFT_1_PENALTY=0.392624
PRIMER_RIGHT_1_PENALTY=0.148509
PRIMER_LEFT_1_SEQUENCE=AGCCATGGAAGCAGAGTAAGAA
PRIMER_RIGHT_1_SEQUENCE=CTCTGGAGACACATGAAAACCA
PRIMER_LEFT_1=697,22
PRIMER_RIGHT_1=919,22
PRIMER_LEFT_1_TM=60.393
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.5411
PRIMER_LEFT_2_PENALTY=0.392624
PRIMER_RIGHT_2_PENALTY=0.148509
PRIMER_LEFT_2_SEQUENCE=AAGCCATGGAAGCAGAGTAAGA
PRIMER_RIGHT_2_SEQUENCE=CTCTGGAGACACATGAAAACCA
PRIMER_LEFT_2=696,22
PRIMER_RIGHT_2=919,22
PRIMER_LEFT_2_TM=60.393
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=224
PRIMER_PAIR_PENALTY_3=0.5411
PRIMER_LEFT_3_PENALTY=0.392624
PRIMER_RIGHT_3_PENALTY=0.148509
PRIMER_LEFT_3_SEQUENCE=GAAGCCATGGAAGCAGAGTAAG
PRIMER_RIGHT_3_SEQUENCE=CTCTGGAGACACATGAAAACCA
PRIMER_LEFT_3=695,22
PRIMER_RIGHT_3=919,22
PRIMER_LEFT_3_TM=60.393
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=0.6299
PRIMER_LEFT_4_PENALTY=0.392624
PRIMER_RIGHT_4_PENALTY=0.237277
PRIMER_LEFT_4_SEQUENCE=GCCATGGAAGCAGAGTAAGAAG
PRIMER_RIGHT_4_SEQUENCE=AGAGAGTGTGGCGTGTATTGTG
PRIMER_LEFT_4=698,22
PRIMER_RIGHT_4=945,22
PRIMER_LEFT_4_TM=60.393
PRIMER_RIGHT_4_TM=60.237
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=248
=
PRIMER_SEQUENCE_ID=SSR_05894 Ps_contig_mira-and-tgicl-ass_franssen_40425
SEQUENCE=AGAGGATTNAGGGTCCGGAACACATCTTCAGTATCGAATTGCAACATAACAATGACGGAAGTGGAGGCAAACGGCGACGGCGAAGCCGGTGTTCCACCGCCGAAGTCGGAAACACAAGATTCCACTGAAAACAACGGAAACTCCTCAGAGCCAGCAGTATCGAAGAGCGCGCTGAAGAAACAAGCGAGACAACAGAGATGGGAGGCAAAGAAGGCAGAGAAGAAAGCAGCGGCGAAGGAACAGAAGAAGAAGGAAACCGAACGGAAACGGAAGGAGTGGGAAGAAAGCCTCGCCAGTATACCTGAGGATGAAAGAACGAAATTCATCGAATCAAGGATTAGTCTCCGTAAGGAGAGAATGGAGCAGAAAACATTGGAGAAAAATAGCAAGAAAGAGAGGCTCAATAAGGGCTAAAGAACAAGGACAAAATGTCGTCGTTGATCTTCAGTTCTCGCATCTCATGACTCCTTCAGAAATCCGCAGTCTCGTTCAACAGATAATGTATTGCTATGCGGTGAATGGAAGGTGTGAGTCCCCTGCACATCTTTGGCTAACAGGCTGCGAAGGAGAGATGGATGATCAATTGAAAAAGATACCAGGATTTGATAAGTGGATAATTGAGAAGGAAAATAAGCCTTATATTGAAGCATTGCAAGATCGTAAGGATGATTTGGTGTATCTAACGGCTGACTCAGAGACTGTTCTTGAAGAGCTTGATTTGAAGAAGATATATATAATTGGCGGGTTAGTGGATAGGAATAGGTGGAAAGGGATAACTATGGAGAAAGCACAAGAGCAAGGAATTCAAACGGCTAAACTCCCTATTTCTAATTTCTTGAAGATGTCTAGTTCTCAGGTTCTTACTGTGAATCAAGTTGTAGAAATACTACTCAAGTTCATTGAGACAAGGGACTGGAAAACTTCTTTCTTCGCAGTTATCCCTCAGAGGAAAAGATCTCAAGCAGATTCAGAAGGAAATACAGATAATGCTGTAGTAGAAGAAGAAGATGAAGAAGAGCGTGAACAAAACGATGATCCATTGACAAAGTAAAAAGCAATGTGTTGAGGAGATCCCTTCTAACAAACTTGTTGATGGCCTGTAGAGTGTAGAACTGGAAAACCAGCGCATGATGAGACAAGTTTCAAATCAGTGCTGCACACAAAATTTTCATACTGGCAGGATTAGCACAACAATTTATGTAGTTATATGTTTTTTCTTTTACCTAACTGTGACCCGATTTTCTTATGATGGTATTGCATTTTTGGATGATTCGTACTCTAGTTCTGTTATCTGATAATGGTGAATGAAAAATATTCCTTGGCGTGCGTTGGCAAACAAGGTACCTATGGTATTCTACATTTGGCAACACATAAATGTTGGCCTTATTTTATGCACCTGCTGTTGGCAAAGTTACTGTTCATACATAATGAGTGTAGTTCAGTATTTGTTTAACTAGCGCTAGTAAACTTTTAAGTTATTAGTAACTTTTAATAGGTTGGAAGGACGGTAAAATTTAGGTTCGTGTTAAG
TARGET=997,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2269
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.203324
PRIMER_LEFT_SEQUENCE=ATTGAGACAAGGGACTGGAAAA
PRIMER_RIGHT_SEQUENCE=CACTCTACAGGCCATCAACAAG
PRIMER_LEFT=898,22
PRIMER_RIGHT=1106,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.797
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=209
PRIMER_PAIR_PENALTY_1=0.2815
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.257927
PRIMER_LEFT_1_SEQUENCE=ATTGAGACAAGGGACTGGAAAA
PRIMER_RIGHT_1_SEQUENCE=TGCTAATCCTGCCAGTATGAAA
PRIMER_LEFT_1=898,22
PRIMER_RIGHT_1=1187,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.742
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=0.3062
PRIMER_LEFT_2_PENALTY=0.003602
PRIMER_RIGHT_2_PENALTY=0.302618
PRIMER_LEFT_2_SEQUENCE=CAAGGAATTCAAACGGCTAAAC
PRIMER_RIGHT_2_SEQUENCE=ATCGTTTTGTTCACGCTCTTCT
PRIMER_LEFT_2=796,22
PRIMER_RIGHT_2=1032,22
PRIMER_LEFT_2_TM=60.004
PRIMER_RIGHT_2_TM=60.303
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=0.3391
PRIMER_LEFT_3_PENALTY=0.036444
PRIMER_RIGHT_3_PENALTY=0.302618
PRIMER_LEFT_3_SEQUENCE=GGATAGGAATAGGTGGAAAGGG
PRIMER_RIGHT_3_SEQUENCE=ATCGTTTTGTTCACGCTCTTCT
PRIMER_LEFT_3=750,22
PRIMER_RIGHT_3=1032,22
PRIMER_LEFT_3_TM=60.036
PRIMER_RIGHT_3_TM=60.303
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.3469
PRIMER_LEFT_4_PENALTY=0.143555
PRIMER_RIGHT_4_PENALTY=0.203324
PRIMER_LEFT_4_SEQUENCE=TCTTTCTTCGCAGTTATCCCTC
PRIMER_RIGHT_4_SEQUENCE=CACTCTACAGGCCATCAACAAG
PRIMER_LEFT_4=922,22
PRIMER_RIGHT_4=1106,22
PRIMER_LEFT_4_TM=59.856
PRIMER_RIGHT_4_TM=59.797
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_05895 Ps_contig_mira-and-tgicl-ass_franssen_40447
SEQUENCE=ACAACACAAATTCATTCATTCATGTCTGTTTCTTCCGCTTCTTCTTCATCATCTTCTCAAAATCATAATTTTCTTCTCATTCTCTCTTTCATTTTTCACATCAACAAAAAAAACAACAAAAACGTTGTAATGGGTCTTTTACTTTCAAGGAGTTTGGCTAGGCAGTGTTAGAAGCTACGCGAAGAATCTTCGCATTGATGGAGTGAAAGATACCATAGCTATTGCTTCCGGTAAAGGCGGTGTCGGCAAATCCACAACTGCTGTGAATTTAGCTGTTGCACTTGCGAGTAAGTTTCAGCTGAAGGTTGGTTTACTTGATGCTGATGTATATGGACCCAACATTCCTATCATGATGAACATCAACACAAAACCTGAAGTCACTCTAGATAAGAAAATGATTCCCATTGATAGTTTGGGATAAAGTGTATGTCGATAGGGTTCCTTGTGGAGAAGAATGCCCCAATTGTCTGGAGAGGTCCCATGGTATCAAACGCTCTTGAGAAAATGACAAGGGGAGTTGACTGGGGTCACCTTGACATTCTAGTGATAGATATGCCCCCTGGTACCGGTGATGTCCAGATATCTATGTCTCAGAATCTGCAATTATCAGGTGCACTGATTGTTTCGACTCCTCAAGATGTTGCATTAATGGATGCGAGGAGGGGAGTACAAATGTTCAATAAAGTTGATATTCCGATTTTGGGAGTTATAGAGAATATGAGCTGCTTCAAGTGTCCACATTGTGGCGAACCTTCATATATATTTGGAAAAGGAGGGGCTCATAGCACAGCCCTTGAAATGGGATTAGAATTTCTTGGCGAGATACCACTGGAAGTGGGGATCAGGGAAGCTTGTGATCAAGGGCACCCAATAGTGCTGGCCGCACCTGATTCGGTAGTTTCCAGAGCATATGGCAATATTGCTGAAAAAGTTGTTCAGAAACTCAAGGAGCGAAAATTTCAACCAGAAATTATACTATGATAACCCTCCGGTCTGTGAACAAGAAAAAGGACCGAGCTGGCTTCCCTTATGCTGGTTAAACTGGGTTTTGACTACTTCTAAATTTTGTTGAAATTGTAGCATGAGATGCGGGCCTGGCATATGATATAGATTTGTAGATTCAGGTATGTTCAATTTTGTTTAATAAGCTGTTAGTGATTCTTTGACGTCACATGGGTGCTGTCAAATTAGCGGTTTAATTTAACTTCAACATTGAGATTACATAGAATTGGAAGCAAAGCAGTGTTTTGTGATGCCATTCCTTAAGCTGTAATGTGACTGTCTTTACGCTTATAATGAAGTACTTAAANCNAAATGATGAACTATTTCGGTATTTTGTTTAATTGTATAAATTNTTTTATTTTGTATTGTTTTATTTACG
TARGET=10,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05896 Ps_contig_mira-and-tgicl-ass_franssen_40460
SEQUENCE=TCGGGGCTCTTAAATCTCTTGCTTCAGCTGTGCAATTCACAGCCACCGAAGCAAGAGAGAAAAAAAACCCTCACAACCAAAACGCCGCGTTTCGTTTCTCGCTATGGTTCCTTCAATTCCCATCACCGACGAAGCCGTTGCCGTTTCTATCGACAGCCCTAAGCAAAAATCAATGAAGAAGAAGAAGGAAAAGCTTCAGAACGACGTCGCTTCCGACCAGGAAACGGCGAAGAAATCTTCAAAGAAGAGGAAGAAAAGTTCCGATGATGATGAGGTGAAGAATGAAACTATCTCTGAGGTTAAGAAGGAGAAGAAGAAGAAAAAGGCGAAGGTGGAAGATGAGGATGTGGAAAAAGAAGCTGTTGTGAAGCAGGATGATCCGAATGCGGTTTCGAATTTTAGAATTTCGGAACCGTTGAAGCTGAAGCTGAAAGAGAAGGGGATTGAGGCTTTGTTCCCTATTCAAGCCATGACTTTTAATACTATTCTTGATGGTTCTGATTTGGTTGGTCGTGCTCGGACTGGTCAGGGAAAAACTCTGGCATTTGTTTTGCCCATATTGGAGTCACTGACAAATGGACTGGCAAAATTGGTTAGAAAGACTGGCTATGGGAGAGTTCCAAGTGTTCTTGTTCTCTTACCTACCAGGGAATTGGCCAATCAGGTGTATGCTGACTTTGAAGTATATGGTGGGTCATTGGGATTGGCTGCTTGCGCTG
TARGET=175,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1189
PRIMER_LEFT_PENALTY=0.019797
PRIMER_RIGHT_PENALTY=0.099075
PRIMER_LEFT_SEQUENCE=TATGGTTCCTTCAATTCCCATC
PRIMER_RIGHT_SEQUENCE=ACCTTCGCCTTTTTCTTCTTCT
PRIMER_LEFT=102,22
PRIMER_RIGHT=332,22
PRIMER_LEFT_TM=60.020
PRIMER_RIGHT_TM=59.901
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.1870
PRIMER_LEFT_1_PENALTY=0.019797
PRIMER_RIGHT_1_PENALTY=0.167242
PRIMER_LEFT_1_SEQUENCE=TATGGTTCCTTCAATTCCCATC
PRIMER_RIGHT_1_SEQUENCE=ACCTCATCATCATCGGAACTTT
PRIMER_LEFT_1=102,22
PRIMER_RIGHT_1=275,22
PRIMER_LEFT_1_TM=60.020
PRIMER_RIGHT_1_TM=59.833
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=174
PRIMER_PAIR_PENALTY_2=0.1945
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.099075
PRIMER_LEFT_2_SEQUENCE=TTCTCGCTATGGTTCCTTCAAT
PRIMER_RIGHT_2_SEQUENCE=ACCTTCGCCTTTTTCTTCTTCT
PRIMER_LEFT_2=95,22
PRIMER_RIGHT_2=332,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=238
PRIMER_PAIR_PENALTY_3=0.1945
PRIMER_LEFT_3_PENALTY=0.095445
PRIMER_RIGHT_3_PENALTY=0.099075
PRIMER_LEFT_3_SEQUENCE=CTCGCTATGGTTCCTTCAATTC
PRIMER_RIGHT_3_SEQUENCE=ACCTTCGCCTTTTTCTTCTTCT
PRIMER_LEFT_3=97,22
PRIMER_RIGHT_3=332,22
PRIMER_LEFT_3_TM=60.095
PRIMER_RIGHT_3_TM=59.901
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=236
PRIMER_PAIR_PENALTY_4=0.2351
PRIMER_LEFT_4_PENALTY=0.135999
PRIMER_RIGHT_4_PENALTY=0.099075
PRIMER_LEFT_4_SEQUENCE=GTTTCGTTTCTCGCTATGGTTC
PRIMER_RIGHT_4_SEQUENCE=ACCTTCGCCTTTTTCTTCTTCT
PRIMER_LEFT_4=88,22
PRIMER_RIGHT_4=332,22
PRIMER_LEFT_4_TM=60.136
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=245
=
PRIMER_SEQUENCE_ID=SSR_05897 Ps_contig_mira-and-tgicl-ass_franssen_40460
SEQUENCE=TCGGGGCTCTTAAATCTCTTGCTTCAGCTGTGCAATTCACAGCCACCGAAGCAAGAGAGAAAAAAAACCCTCACAACCAAAACGCCGCGTTTCGTTTCTCGCTATGGTTCCTTCAATTCCCATCACCGACGAAGCCGTTGCCGTTTCTATCGACAGCCCTAAGCAAAAATCAATGAAGAAGAAGAAGGAAAAGCTTCAGAACGACGTCGCTTCCGACCAGGAAACGGCGAAGAAATCTTCAAAGAAGAGGAAGAAAAGTTCCGATGATGATGAGGTGAAGAATGAAACTATCTCTGAGGTTAAGAAGGAGAAGAAGAAGAAAAAGGCGAAGGTGGAAGATGAGGATGTGGAAAAAGAAGCTGTTGTGAAGCAGGATGATCCGAATGCGGTTTCGAATTTTAGAATTTCGGAACCGTTGAAGCTGAAGCTGAAAGAGAAGGGGATTGAGGCTTTGTTCCCTATTCAAGCCATGACTTTTAATACTATTCTTGATGGTTCTGATTTGGTTGGTCGTGCTCGGACTGGTCAGGGAAAAACTCTGGCATTTGTTTTGCCCATATTGGAGTCACTGACAAATGGACTGGCAAAATTGGTTAGAAAGACTGGCTATGGGAGAGTTCCAAGTGTTCTTGTTCTCTTACCTACCAGGGAATTGGCCAATCAGGTGTATGCTGACTTTGAAGTATATGGTGGGTCATTGGGATTGGCTGCTTGCGCTG
TARGET=309,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2013
PRIMER_LEFT_PENALTY=0.167242
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=AAAGTTCCGATGATGATGAGGT
PRIMER_RIGHT_SEQUENCE=AAGTCATGGCTTGAATAGGGAA
PRIMER_LEFT=254,22
PRIMER_RIGHT=475,22
PRIMER_LEFT_TM=59.833
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.2013
PRIMER_LEFT_1_PENALTY=0.167242
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=AAAGTTCCGATGATGATGAGGT
PRIMER_RIGHT_1_SEQUENCE=AAAGTCATGGCTTGAATAGGGA
PRIMER_LEFT_1=254,22
PRIMER_RIGHT_1=476,22
PRIMER_LEFT_1_TM=59.833
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=223
PRIMER_PAIR_PENALTY_2=0.2613
PRIMER_LEFT_2_PENALTY=0.019797
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=TATGGTTCCTTCAATTCCCATC
PRIMER_RIGHT_2_SEQUENCE=CAGCTTCTTTTTCCACATCCTC
PRIMER_LEFT_2=102,22
PRIMER_RIGHT_2=361,22
PRIMER_LEFT_2_TM=60.020
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.2613
PRIMER_LEFT_3_PENALTY=0.019797
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=TATGGTTCCTTCAATTCCCATC
PRIMER_RIGHT_3_SEQUENCE=AGCTTCTTTTTCCACATCCTCA
PRIMER_LEFT_3=102,22
PRIMER_RIGHT_3=360,22
PRIMER_LEFT_3_TM=60.020
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.3189
PRIMER_LEFT_4_PENALTY=0.019797
PRIMER_RIGHT_4_PENALTY=0.299089
PRIMER_LEFT_4_SEQUENCE=TATGGTTCCTTCAATTCCCATC
PRIMER_RIGHT_4_SEQUENCE=TTTTTCCACATCCTCATCTTCC
PRIMER_LEFT_4=102,22
PRIMER_RIGHT_4=354,22
PRIMER_LEFT_4_TM=60.020
PRIMER_RIGHT_4_TM=60.299
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_05898 Ps_contig_mira-and-tgicl-ass_franssen_40464
SEQUENCE=CGGGGAGATATCTTGTGATCCTATTCTCTCCCTCTCTTCATCTCTTATATATTAGTGGACAAAAAGATCAAACAAACCGTTTCAGTTACCTCTTCTCTATTTTTTCTTTGTATGCATCATGTTACCACCACCTTATCACCTTATGCCTCTAGATCTTAATCAAGATCAAAACCATAATCATCTTTCTTCTTCTTCTTCTTCATTTTCTTCTCCATCATCATCTTATCCTAACCCGCCGGAACAAGTTCGACAAGAACCGTCTTATTACTTCGAAACAAAGCATGATCCTGAAGAGGTTGAGAAGACTATTCCATCAAGTGGATCATGGAATTCTTCAACTCCAGAAAACCATGAGAGCGGTAGAATTAAACACAAGCTTACAATTCGTTGGAAGAAAGAAGAAACTAGTACCGACGAAACTCGTAATATTCGAGCTGATGATGATCAAGAGGATGGTGCTTCAATGAAGTGGATGTCATCAAAGATGAGAATAATGAAGAAGATGATGGTTTCTGATCCTCAAACCGGTGATATTAAGCCGATTAAGTATGAAGATCACCAACAACAAGTTACCGATAACAGCAGCAACAACAGCAACAATAATTACTCGACTCATCTCAGCTATAATAATAGCCCTACTATTAGGGTTTGTTCTGATTGCAACACCACTAAGACCCCTCTCTGGAGGAGTGGACCAAGAGGCCCCAAGTCACTTTGCAACGCGTGTGGGATTAGACAAAGGAAGGCGAGGCGCGCGATGGCATTGGCTGCCGCGTCTGGAAACGGAACAACAATTGCTGATCACGAGACATCT
TARGET=184,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3091
PRIMER_LEFT_PENALTY=0.143555
PRIMER_RIGHT_PENALTY=0.165541
PRIMER_LEFT_SEQUENCE=CCACCTTATCACCTTATGCCTC
PRIMER_RIGHT_SEQUENCE=TCCAACGAATTGTAAGCTTGTG
PRIMER_LEFT=127,22
PRIMER_RIGHT=391,22
PRIMER_LEFT_TM=59.856
PRIMER_RIGHT_TM=60.166
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=265
PRIMER_PAIR_PENALTY_1=0.3529
PRIMER_LEFT_1_PENALTY=0.143555
PRIMER_RIGHT_1_PENALTY=0.209343
PRIMER_LEFT_1_SEQUENCE=CCACCTTATCACCTTATGCCTC
PRIMER_RIGHT_1_SEQUENCE=AGACGGTTCTTGTCGAACTTGT
PRIMER_LEFT_1=127,22
PRIMER_RIGHT_1=261,22
PRIMER_LEFT_1_TM=59.856
PRIMER_RIGHT_1_TM=60.209
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=0.3866
PRIMER_LEFT_2_PENALTY=0.143555
PRIMER_RIGHT_2_PENALTY=0.243031
PRIMER_LEFT_2_SEQUENCE=CCACCTTATCACCTTATGCCTC
PRIMER_RIGHT_2_SEQUENCE=GCTCTCATGGTTTTCTGGAGTT
PRIMER_LEFT_2=127,22
PRIMER_RIGHT_2=357,22
PRIMER_LEFT_2_TM=59.856
PRIMER_RIGHT_2_TM=59.757
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.4056
PRIMER_LEFT_3_PENALTY=0.143555
PRIMER_RIGHT_3_PENALTY=0.261998
PRIMER_LEFT_3_SEQUENCE=CCACCTTATCACCTTATGCCTC
PRIMER_RIGHT_3_SEQUENCE=TCTACCGCTCTCATGGTTTTCT
PRIMER_LEFT_3=127,22
PRIMER_RIGHT_3=363,22
PRIMER_LEFT_3_TM=59.856
PRIMER_RIGHT_3_TM=60.262
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=0.4303
PRIMER_LEFT_4_PENALTY=0.264777
PRIMER_RIGHT_4_PENALTY=0.165541
PRIMER_LEFT_4_SEQUENCE=TGTATGCATCATGTTACCACCA
PRIMER_RIGHT_4_SEQUENCE=TCCAACGAATTGTAAGCTTGTG
PRIMER_LEFT_4=108,22
PRIMER_RIGHT_4=391,22
PRIMER_LEFT_4_TM=59.735
PRIMER_RIGHT_4_TM=60.166
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_05899 Ps_contig_mira-and-tgicl-ass_franssen_40468
SEQUENCE=TCGATGTTGTTGTTGTTGTTGTTGTCACTATCATGTCTTTGATCAGAAGTGTCGGTGCTATGGAACAAATGCTAATTCTTTTGTAACTGTTACCAGGTGGACACTGAATACGTTAGGCAGCAGCGGAAAAAATTAGGGGACAGAGGTTGTTGCATGGAGTAAGGGTGTGATTGGCAATGCAGAGAAACCTATTGTTATTAGTGGTCCTTCTGGAGTCGGTAAAGGAACACTGATATCAATGCTCATGAAGGAATTCCCATCGATGTTTGGTTTTTTCTGTGAGCCACACCACTCGTGCTCCGCGGGATATGGAGAAGAATGGGGTCCATTACCATTTTACTGAGAAGAGTGTGATGGAGAAAGAGATTAAAAATGGAAAGTTTCTCGAGTTTGCTTCTGTACATGGTAATTTGTATGGGACCAGTGTTGAAGCTGTTGAAGTTGTGGCTGATGCAGGAAAAAGATGTATTCTTGATATTGATGTTCAAGGAGCAAGATCAGTGAAGGCTAGTTCTCTTGAAGCCATATTCATCTTTATCTGCCCCCCATCAATGGAAGAGCTTGAGAAGCGCCTTCGTGACAGAGGGACTGAGACAGAGGAGCAGATCCTTAAGCGACTGCGTAACGCCTCGGCTGAAATCGAACAAGGAAAGTCTTCCAATATATTCGATTTCACCTTATACAATGACAATCTTGAGGAGTCTTATGAGAGACTTAAGAAATTATTGGGACTTAATGGTTTTGTCACTGCTTCACCGAAATCAGCCGCACCTAGAGAGATAAATTTACCAATGGATCATTCGGTGTCTAAACTCGATGACAAAATCATCATAAACTGCATCTCTTCTGGACCGGATAAAGAGTCAAAGAACTTGATCATGTTAGATGTTTCCTCACTAAAAGGGGGTGCACCTGGAAGGACAAGAGGGCTTGATTTTCATGTTATAGGCTCTATGGATCAACTTAGCTAACATTCTATGAATTTTAGCCACAACTAACTTGGTTTTCTCCATTCATAATTGTTACCTATAAGGCATTAGATTTATGAGTTGAAACATTTTTTTTCCTTTCTCACTGGCGTTCTTTGATTGTTTATGGCAATTGAATTGATTTTGAGTAGTGTTGGCAATGACACATTTATTATTATTATTCCCTACATGGGCAATGAAATGATTTTTGTAAGG
TARGET=5,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05900 Ps_contig_mira-and-tgicl-ass_franssen_40468
SEQUENCE=TCGATGTTGTTGTTGTTGTTGTTGTCACTATCATGTCTTTGATCAGAAGTGTCGGTGCTATGGAACAAATGCTAATTCTTTTGTAACTGTTACCAGGTGGACACTGAATACGTTAGGCAGCAGCGGAAAAAATTAGGGGACAGAGGTTGTTGCATGGAGTAAGGGTGTGATTGGCAATGCAGAGAAACCTATTGTTATTAGTGGTCCTTCTGGAGTCGGTAAAGGAACACTGATATCAATGCTCATGAAGGAATTCCCATCGATGTTTGGTTTTTTCTGTGAGCCACACCACTCGTGCTCCGCGGGATATGGAGAAGAATGGGGTCCATTACCATTTTACTGAGAAGAGTGTGATGGAGAAAGAGATTAAAAATGGAAAGTTTCTCGAGTTTGCTTCTGTACATGGTAATTTGTATGGGACCAGTGTTGAAGCTGTTGAAGTTGTGGCTGATGCAGGAAAAAGATGTATTCTTGATATTGATGTTCAAGGAGCAAGATCAGTGAAGGCTAGTTCTCTTGAAGCCATATTCATCTTTATCTGCCCCCCATCAATGGAAGAGCTTGAGAAGCGCCTTCGTGACAGAGGGACTGAGACAGAGGAGCAGATCCTTAAGCGACTGCGTAACGCCTCGGCTGAAATCGAACAAGGAAAGTCTTCCAATATATTCGATTTCACCTTATACAATGACAATCTTGAGGAGTCTTATGAGAGACTTAAGAAATTATTGGGACTTAATGGTTTTGTCACTGCTTCACCGAAATCAGCCGCACCTAGAGAGATAAATTTACCAATGGATCATTCGGTGTCTAAACTCGATGACAAAATCATCATAAACTGCATCTCTTCTGGACCGGATAAAGAGTCAAAGAACTTGATCATGTTAGATGTTTCCTCACTAAAAGGGGGTGCACCTGGAAGGACAAGAGGGCTTGATTTTCATGTTATAGGCTCTATGGATCAACTTAGCTAACATTCTATGAATTTTAGCCACAACTAACTTGGTTTTCTCCATTCATAATTGTTACCTATAAGGCATTAGATTTATGAGTTGAAACATTTTTTTTCCTTTCTCACTGGCGTTCTTTGATTGTTTATGGCAATTGAATTGATTTTGAGTAGTGTTGGCAATGACACATTTATTATTATTATTCCCTACATGGGCAATGAAATGATTTTTGTAAGG
TARGET=1138,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3321
PRIMER_LEFT_PENALTY=0.401769
PRIMER_RIGHT_PENALTY=0.930315
PRIMER_LEFT_SEQUENCE=GACAAGAGGGCTTGATTTTCAT
PRIMER_RIGHT_SEQUENCE=ATCATTTCATTGCCCATGTAGG
PRIMER_LEFT=919,22
PRIMER_RIGHT=1173,22
PRIMER_LEFT_TM=59.598
PRIMER_RIGHT_TM=60.930
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=255
PRIMER_PAIR_PENALTY_1=1.3957
PRIMER_LEFT_1_PENALTY=0.465395
PRIMER_RIGHT_1_PENALTY=0.930315
PRIMER_LEFT_1_SEQUENCE=AAGGACAAGAGGGCTTGATTTT
PRIMER_RIGHT_1_SEQUENCE=ATCATTTCATTGCCCATGTAGG
PRIMER_LEFT_1=916,22
PRIMER_RIGHT_1=1173,22
PRIMER_LEFT_1_TM=60.465
PRIMER_RIGHT_1_TM=60.930
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=1.4131
PRIMER_LEFT_2_PENALTY=0.482784
PRIMER_RIGHT_2_PENALTY=0.930315
PRIMER_LEFT_2_SEQUENCE=CAAGAGGGCTTGATTTTCATGT
PRIMER_RIGHT_2_SEQUENCE=ATCATTTCATTGCCCATGTAGG
PRIMER_LEFT_2=921,22
PRIMER_RIGHT_2=1173,22
PRIMER_LEFT_2_TM=60.483
PRIMER_RIGHT_2_TM=60.930
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=1.4925
PRIMER_LEFT_3_PENALTY=0.562203
PRIMER_RIGHT_3_PENALTY=0.930315
PRIMER_LEFT_3_SEQUENCE=ATGTTTCCTCACTAAAAGGGGG
PRIMER_RIGHT_3_SEQUENCE=ATCATTTCATTGCCCATGTAGG
PRIMER_LEFT_3=885,22
PRIMER_RIGHT_3=1173,22
PRIMER_LEFT_3_TM=60.562
PRIMER_RIGHT_3_TM=60.930
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=12.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=1.8767
PRIMER_LEFT_4_PENALTY=0.946348
PRIMER_RIGHT_4_PENALTY=0.930315
PRIMER_LEFT_4_SEQUENCE=GAAGGACAAGAGGGCTTGATTT
PRIMER_RIGHT_4_SEQUENCE=ATCATTTCATTGCCCATGTAGG
PRIMER_LEFT_4=915,22
PRIMER_RIGHT_4=1173,22
PRIMER_LEFT_4_TM=60.946
PRIMER_RIGHT_4_TM=60.930
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=259
=
PRIMER_SEQUENCE_ID=SSR_05901 Ps_contig_mira-and-tgicl-ass_franssen_40482
SEQUENCE=CCTTTAACCTAAAAAAACGAAAATACAACCTTTTTAATTTGTAGGTTTAAATTTACTTTACCTACTAAATAACTACAAATAACGACATCCTCCAGAAGGAGTCTAAATTTTAAAATTACCTTTCTACCAATTTAAAATTAGCTTTGTCACAGGATGAAAGTAGCTCTACTCCAAAGATTGATTAATGCTTGGAATGCTGTTTTCATCGTACAAATTGACAGCAACCAGGACACAATTTTGACAGCCACAAGTGCTTCAACTTCACTATTAAGGCAAAGATGGCATAGCATGGAATATGCATTATACCTACAGCCAAGTACCATAAGGGTGCAAATGGAGATGACAGATCAAGGAAAGGATAAGGCCACCAAAGAGAAACAACGGCGTGGATGATCCACTGAAAAATCACAAATGTAGCCGTCCACAAGATAAAATATGCGAATCGAAACACTGGAAAGCTCATGGAGTTCAATGATGTTTCACCAAGGAGGAGCACAGCATTAACAGAGTGCATACTAACAGTGAATATATCGAGACGGAAGTCTTTGGGTGTCATAAAAGGATAAAGAACGAGCCAGAATACACTGTCAGTGAGCACCGAAGCACCTGCACAAGTCTGAAATATTATTTGGATAATATATCCCCATACACCAGCAGTATTATGATTGATAGTCTCCCGAGAGGCATAGGAACTCTTAGATAAGATAGGTACATCTGCAATTCCATCAAGTGTAGGAGCAACATAAGAGCCTCGTTCTGCGTCTAATGTATTTCCCTCAAATTTTGTGTGGTTAAAGTAAATACAATAGAAGGAGAAACAAGATGCAACCGCAAAGTAAATAGTAACCAAAGTAAAGGTCCACTGAGTGTAGAAGTAAAATATGCCACCACCAGCAACAGCTACATTCGCAACGAGCAAACCCAAAAGCACAAGAAAAGAAATTATTCTATAAGCAAGAAGCCATTTAGGATTAATTCCTTTAACACAAGTATTCCACGCTTCATCTTCATATAACAATCCAACCGTTTCTCGCCGCCCTTCTTCAACTACCCTCTCATTTCTTGATCTATTGAATTCCTCATACTTCCATATCAAAAATGATCCCAATCCCATACTGAATAAAATAAATAGCGCAGAAAGGAAGAATCTCCAATTCAACCAGTAGCTTAAAGTATTGGTATCTTGTGTCATGGTGGCTATATTTCCCAAGTATCACTTGAGAATCAGTTCAGAGAGAGAGAGAAAGAGAGGGGGGTATATGCAGTATGAAGATGTGATGAATCACACACACACCAACCAATGACCTGCCTCTTCTGCCTCTCCT
TARGET=1232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2948
PRIMER_LEFT_PENALTY=0.236697
PRIMER_RIGHT_PENALTY=0.058146
PRIMER_LEFT_SEQUENCE=ATAACAATCCAACCGTTTCTCG
PRIMER_RIGHT_SEQUENCE=ATTGGTTGGTGTGTGTGTGATT
PRIMER_LEFT=1011,22
PRIMER_RIGHT=1301,22
PRIMER_LEFT_TM=60.237
PRIMER_RIGHT_TM=60.058
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.3127
PRIMER_LEFT_1_PENALTY=0.254567
PRIMER_RIGHT_1_PENALTY=0.058146
PRIMER_LEFT_1_SEQUENCE=CCCTTCTTCAACTACCCTCTCA
PRIMER_RIGHT_1_SEQUENCE=ATTGGTTGGTGTGTGTGTGATT
PRIMER_LEFT_1=1036,22
PRIMER_RIGHT_1=1301,22
PRIMER_LEFT_1_TM=59.745
PRIMER_RIGHT_1_TM=60.058
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.4569
PRIMER_LEFT_2_PENALTY=0.398742
PRIMER_RIGHT_2_PENALTY=0.058146
PRIMER_LEFT_2_SEQUENCE=TGATCCCAATCCCATACTGAAT
PRIMER_RIGHT_2_SEQUENCE=ATTGGTTGGTGTGTGTGTGATT
PRIMER_LEFT_2=1099,22
PRIMER_RIGHT_2=1301,22
PRIMER_LEFT_2_TM=60.399
PRIMER_RIGHT_2_TM=60.058
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=203
PRIMER_PAIR_PENALTY_3=0.6157
PRIMER_LEFT_3_PENALTY=0.557601
PRIMER_RIGHT_3_PENALTY=0.058146
PRIMER_LEFT_3_SEQUENCE=CATGGTGGCTATATTTCCCAAG
PRIMER_RIGHT_3_SEQUENCE=ATTGGTTGGTGTGTGTGTGATT
PRIMER_LEFT_3=1189,22
PRIMER_RIGHT_3=1301,22
PRIMER_LEFT_3_TM=60.558
PRIMER_RIGHT_3_TM=60.058
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=113
PRIMER_PAIR_PENALTY_4=0.6635
PRIMER_LEFT_4_PENALTY=0.605368
PRIMER_RIGHT_4_PENALTY=0.058146
PRIMER_LEFT_4_SEQUENCE=GCCCTTCTTCAACTACCCTCTC
PRIMER_RIGHT_4_SEQUENCE=ATTGGTTGGTGTGTGTGTGATT
PRIMER_LEFT_4=1035,22
PRIMER_RIGHT_4=1301,22
PRIMER_LEFT_4_TM=60.605
PRIMER_RIGHT_4_TM=60.058
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_05902 Ps_contig_mira-and-tgicl-ass_franssen_40482
SEQUENCE=CCTTTAACCTAAAAAAACGAAAATACAACCTTTTTAATTTGTAGGTTTAAATTTACTTTACCTACTAAATAACTACAAATAACGACATCCTCCAGAAGGAGTCTAAATTTTAAAATTACCTTTCTACCAATTTAAAATTAGCTTTGTCACAGGATGAAAGTAGCTCTACTCCAAAGATTGATTAATGCTTGGAATGCTGTTTTCATCGTACAAATTGACAGCAACCAGGACACAATTTTGACAGCCACAAGTGCTTCAACTTCACTATTAAGGCAAAGATGGCATAGCATGGAATATGCATTATACCTACAGCCAAGTACCATAAGGGTGCAAATGGAGATGACAGATCAAGGAAAGGATAAGGCCACCAAAGAGAAACAACGGCGTGGATGATCCACTGAAAAATCACAAATGTAGCCGTCCACAAGATAAAATATGCGAATCGAAACACTGGAAAGCTCATGGAGTTCAATGATGTTTCACCAAGGAGGAGCACAGCATTAACAGAGTGCATACTAACAGTGAATATATCGAGACGGAAGTCTTTGGGTGTCATAAAAGGATAAAGAACGAGCCAGAATACACTGTCAGTGAGCACCGAAGCACCTGCACAAGTCTGAAATATTATTTGGATAATATATCCCCATACACCAGCAGTATTATGATTGATAGTCTCCCGAGAGGCATAGGAACTCTTAGATAAGATAGGTACATCTGCAATTCCATCAAGTGTAGGAGCAACATAAGAGCCTCGTTCTGCGTCTAATGTATTTCCCTCAAATTTTGTGTGGTTAAAGTAAATACAATAGAAGGAGAAACAAGATGCAACCGCAAAGTAAATAGTAACCAAAGTAAAGGTCCACTGAGTGTAGAAGTAAAATATGCCACCACCAGCAACAGCTACATTCGCAACGAGCAAACCCAAAAGCACAAGAAAAGAAATTATTCTATAAGCAAGAAGCCATTTAGGATTAATTCCTTTAACACAAGTATTCCACGCTTCATCTTCATATAACAATCCAACCGTTTCTCGCCGCCCTTCTTCAACTACCCTCTCATTTCTTGATCTATTGAATTCCTCATACTTCCATATCAAAAATGATCCCAATCCCATACTGAATAAAATAAATAGCGCAGAAAGGAAGAATCTCCAATTCAACCAGTAGCTTAAAGTATTGGTATCTTGTGTCATGGTGGCTATATTTCCCAAGTATCACTTGAGAATCAGTTCAGAGAGAGAGAGAAAGAGAGGGGGGTATATGCAGTATGAAGATGTGATGAATCACACACACACCAACCAATGACCTGCCTCTTCTGCCTCTCCT
TARGET=1284,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2152
PRIMER_LEFT_PENALTY=0.090580
PRIMER_RIGHT_PENALTY=1.124598
PRIMER_LEFT_SEQUENCE=AAATGATCCCAATCCCATACTG
PRIMER_RIGHT_SEQUENCE=AGAAGAGGCAGGTCATTGGTT
PRIMER_LEFT=1096,22
PRIMER_RIGHT=1315,21
PRIMER_LEFT_TM=59.909
PRIMER_RIGHT_TM=60.125
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=1.2404
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=1.124598
PRIMER_LEFT_1_SEQUENCE=TAGCGCAGAAAGGAAGAATCTC
PRIMER_RIGHT_1_SEQUENCE=AGAAGAGGCAGGTCATTGGTT
PRIMER_LEFT_1=1129,22
PRIMER_RIGHT_1=1315,21
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=60.125
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=1.3792
PRIMER_LEFT_2_PENALTY=0.254567
PRIMER_RIGHT_2_PENALTY=1.124598
PRIMER_LEFT_2_SEQUENCE=CCCTTCTTCAACTACCCTCTCA
PRIMER_RIGHT_2_SEQUENCE=AGAAGAGGCAGGTCATTGGTT
PRIMER_LEFT_2=1036,22
PRIMER_RIGHT_2=1315,21
PRIMER_LEFT_2_TM=59.745
PRIMER_RIGHT_2_TM=60.125
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=1.5233
PRIMER_LEFT_3_PENALTY=0.398742
PRIMER_RIGHT_3_PENALTY=1.124598
PRIMER_LEFT_3_SEQUENCE=ATGATCCCAATCCCATACTGAA
PRIMER_RIGHT_3_SEQUENCE=AGAAGAGGCAGGTCATTGGTT
PRIMER_LEFT_3=1098,22
PRIMER_RIGHT_3=1315,21
PRIMER_LEFT_3_TM=60.399
PRIMER_RIGHT_3_TM=60.125
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=218
PRIMER_PAIR_PENALTY_4=1.5233
PRIMER_LEFT_4_PENALTY=0.398742
PRIMER_RIGHT_4_PENALTY=1.124598
PRIMER_LEFT_4_SEQUENCE=AATGATCCCAATCCCATACTGA
PRIMER_RIGHT_4_SEQUENCE=AGAAGAGGCAGGTCATTGGTT
PRIMER_LEFT_4=1097,22
PRIMER_RIGHT_4=1315,21
PRIMER_LEFT_4_TM=60.399
PRIMER_RIGHT_4_TM=60.125
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_05903 Ps_contig_mira-and-tgicl-ass_franssen_40484
SEQUENCE=ATCCACAAAAATTATCACTAAGTCATAAGTCAAAGTCTTCTCATTACTCTCCCTCCAATAAAAGAAGAGAAACTAAACAAACAAACAAAATATAGTACCCAAGAATCTTCACATAATATTGTTTTTGAAGGTTTAGGAGACATGGAAAGTAAAAACAATCTCAAGCAGAAGCAGAAGCTAGGGGTATTTGATATACTCAAAGAAGCTATTACCTTATATGTTAGAAATCTCAACTTTATCATCTTCACCTTTCTTACTTCTCTTCCTCTCTTTTGCATAATGGTTTTACTTTTGTAAATTACAGCTCCCAAAAAAACCTTTAAATTTGTAAATTTTTTACTATTGTTGTAAACAA
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7079
PRIMER_LEFT_PENALTY=1.642559
PRIMER_RIGHT_PENALTY=0.065292
PRIMER_LEFT_SEQUENCE=TCTTCTCATTACTCTCCCTCCAA
PRIMER_RIGHT_SEQUENCE=AGCTTCTGCTTCTGCTTGAGAT
PRIMER_LEFT=35,23
PRIMER_RIGHT=178,22
PRIMER_LEFT_TM=59.357
PRIMER_RIGHT_TM=59.935
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=1.9326
PRIMER_LEFT_1_PENALTY=1.642559
PRIMER_RIGHT_1_PENALTY=0.290000
PRIMER_LEFT_1_SEQUENCE=TCTTCTCATTACTCTCCCTCCAA
PRIMER_RIGHT_1_SEQUENCE=GCTTCTGCTTCTGCTTGAGATT
PRIMER_LEFT_1=35,23
PRIMER_RIGHT_1=177,22
PRIMER_LEFT_1_TM=59.357
PRIMER_RIGHT_1_TM=60.290
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=143
PRIMER_PAIR_PENALTY_2=1.9659
PRIMER_LEFT_2_PENALTY=1.642559
PRIMER_RIGHT_2_PENALTY=0.323355
PRIMER_LEFT_2_SEQUENCE=TCTTCTCATTACTCTCCCTCCAA
PRIMER_RIGHT_2_SEQUENCE=TAGCTTCTGCTTCTGCTTGAGA
PRIMER_LEFT_2=35,23
PRIMER_RIGHT_2=179,22
PRIMER_LEFT_2_TM=59.357
PRIMER_RIGHT_2_TM=59.677
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=2.0636
PRIMER_LEFT_3_PENALTY=1.998311
PRIMER_RIGHT_3_PENALTY=0.065292
PRIMER_LEFT_3_SEQUENCE=TCTTCTCATTACTCTCCCTCCA
PRIMER_RIGHT_3_SEQUENCE=AGCTTCTGCTTCTGCTTGAGAT
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=178,22
PRIMER_LEFT_3_TM=58.002
PRIMER_RIGHT_3_TM=59.935
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=2.1939
PRIMER_LEFT_4_PENALTY=2.128624
PRIMER_RIGHT_4_PENALTY=0.065292
PRIMER_LEFT_4_SEQUENCE=GTCTTCTCATTACTCTCCCTCCAA
PRIMER_RIGHT_4_SEQUENCE=AGCTTCTGCTTCTGCTTGAGAT
PRIMER_LEFT_4=34,24
PRIMER_RIGHT_4=178,22
PRIMER_LEFT_4_TM=60.129
PRIMER_RIGHT_4_TM=59.935
PRIMER_LEFT_4_GC_PERCENT=45.833
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_05904 Ps_contig_mira-and-tgicl-ass_franssen_40488
SEQUENCE=AAGCANGGTAGTCAACGCNNAGTNNNGGGGGAACAAACATGTTTTCCAGAACCCGTTCTCTCCTTCCTTCTCTAACATCCCTCCACAAATCCCCAAACCCATCATCATCACTCTTTTCTTCTTCTTCACAACCCCCAATCACAAACACAAACAACAAAGACAACATCATTATATCCAGATTCCACCACAAAGACTGGTTAACCTCAAAACAAGCAACAACCTTAATCAACTCCTTAACTAACCCTTCCACCGCCCTAACTCTCTTCAACCTCTACTCATCCCGCAAAGACTACAACCCAACTCAACCCTTCTGCATTTCCCTCATCACCAAACTAGTTCAAGCACGTCAATTCTCCCCAATCGACACCCTTCTCAAATCACTCCCACAACAACAACGACACCGTTTCACCCAAGATTTCTTCTTCCATCTCATCAAACTCTACGCTCATCAAGCCCACAGAATCGATAAAGCCGTCGAAACACTCTTCATGATGCCCAATTTCGGTTCATGGCCAACCTCTAAAACATTCAACTTCGTTCTTAATCTCTTGGTCAATAACAAACTACACGACCGTCCGTTTTACAATTCTTTATTCTYCGGCTTCCAAGTTAGGATTCCAAATCGA
TARGET=116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05905 Ps_contig_mira-and-tgicl-ass_franssen_40492
SEQUENCE=TTCTCGCTTCTGTCATCCGGGTTTGGATTCTATTACGGACACAGTCACTGGAAATGGGGACACTTGGTTCTTTTTGGTGCCATTCAGAGCACGGCAACAGGCATTCTCCACGCTTTCGGTCGGGTTTTGGTATTGGATTGTGCTCCATCAGGCAAAGAAGGTGCTTTTTCGATATGGTATGCGTGGATGAGAGCTGCAGGGTTATGCGTTGGGTTCACAGTCGGTTCAGTTGGGCCCGGACGGATTAGAACTTCATTCGGAGTCGCGTTTTGCACTGCTATAGCCGGAATTGTCGTGTTACTCTTCGGAAATATCAGCGATGCTGGTGGCGCCGCTGCTGCAGGGAACGTAACCGACGATAACGAAAGAAGTTCCGCAGTTGTTTCTGGTTTGGATTCGAAAGAAGCAGCTCGAGTTTGAATATTAACCGAATTAAATAAAAAAAACTAAATGAATGAATGGATTGAAAGGATGGATGAATGGTGGTGCATGGTATATTGTCTATGCATGATATGATTCTTTGCAAATGTGATGTTAATGCATGCAACAGCAACAAAAAATRGGGGGTTTAGTTTATTTTGTTATGATTGTATTTTAGTTTAGGTTTATTTTGTTTGTCAGAGTGCTTA
TARGET=450,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05906 Ps_contig_mira-and-tgicl-ass_franssen_40498
SEQUENCE=AAGATTGATAAAGAAGAATGGAGAAACCTTGTTTTGCGCCATCCATCACTTCTCAAGAACATGACCCTTCAATATCTAAGGGACATCACCACAACATTCCCAAGCTTTGTATTCCATTCACAAGTAGACGATTCTTGAAGCATAAGTAATATTTACTTGATATGTAAAAAGGGTTATTGCAGAGAGAATTTAATTTAATTGTCATGCTATTTGGTTTGGTTTACTTTTCTTTCATGAACTATTGGTTTTGGGTTGGTTTGGTTTGGTTTTAACGTCCTTACAAAGTAATTACTATTCGTATTTTAGTTGGTTAG
TARGET=253,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05907 Ps_contig_mira-and-tgicl-ass_franssen_40500
SEQUENCE=CCTAACCAATGAAGCATGTGTTNTTCCCAACTGGAACTTTCTGTTTCCAACTTCATCAGCCACACTCCAAATACCTTGTCAAACAATCTACCTTGAACTTTACCATTCCTTCTGATTTTCCTAGGCAATTCAACTTTCTCGAAACCTAACAAGTGTTTCTGAGGCGCATTGTGCACATCCGGAGGTGTAAAGAATAACAAAACTGTATGGCTACTACTCATTTTCGCCGTGTTTTTGACACTAAGGTGAATATCAAATGCCAAGTTTTGACAATGCTCGTCGGCAGTATCAAGTGACTTGCATTGACAAGGGAACGGCTAGTTTCGGTGACTTCCCTCGAAGGCGGAAGGAAGGAACCTGAACCAACCGTAAGGTTGGGGGTTCTTACTTTTTAACTTAGTTTT
TARGET=345,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6713
PRIMER_LEFT_PENALTY=0.061818
PRIMER_RIGHT_PENALTY=0.609530
PRIMER_LEFT_SEQUENCE=ACTACTCATTTTCGCCGTGTTT
PRIMER_RIGHT_SEQUENCE=AGTAAGAACCCCCAACCTTACG
PRIMER_LEFT=212,22
PRIMER_RIGHT=388,22
PRIMER_LEFT_TM=60.062
PRIMER_RIGHT_TM=60.610
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=177
PRIMER_PAIR_PENALTY_1=0.7253
PRIMER_LEFT_1_PENALTY=0.115769
PRIMER_RIGHT_1_PENALTY=0.609530
PRIMER_LEFT_1_SEQUENCE=GCAATTCAACTTTCTCGAAACC
PRIMER_RIGHT_1_SEQUENCE=AGTAAGAACCCCCAACCTTACG
PRIMER_LEFT_1=124,22
PRIMER_RIGHT_1=388,22
PRIMER_LEFT_1_TM=60.116
PRIMER_RIGHT_1_TM=60.610
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.7450
PRIMER_LEFT_2_PENALTY=0.061818
PRIMER_RIGHT_2_PENALTY=0.683230
PRIMER_LEFT_2_SEQUENCE=ACTACTCATTTTCGCCGTGTTT
PRIMER_RIGHT_2_SEQUENCE=AAAAAGTAAGAACCCCCAACCT
PRIMER_LEFT_2=212,22
PRIMER_RIGHT_2=392,22
PRIMER_LEFT_2_TM=60.062
PRIMER_RIGHT_2_TM=59.317
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=181
PRIMER_PAIR_PENALTY_3=0.7450
PRIMER_LEFT_3_PENALTY=0.061818
PRIMER_RIGHT_3_PENALTY=0.683230
PRIMER_LEFT_3_SEQUENCE=ACTACTCATTTTCGCCGTGTTT
PRIMER_RIGHT_3_SEQUENCE=AAAAGTAAGAACCCCCAACCTT
PRIMER_LEFT_3=212,22
PRIMER_RIGHT_3=391,22
PRIMER_LEFT_3_TM=60.062
PRIMER_RIGHT_3_TM=59.317
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.7990
PRIMER_LEFT_4_PENALTY=0.115769
PRIMER_RIGHT_4_PENALTY=0.683230
PRIMER_LEFT_4_SEQUENCE=GCAATTCAACTTTCTCGAAACC
PRIMER_RIGHT_4_SEQUENCE=AAAAAGTAAGAACCCCCAACCT
PRIMER_LEFT_4=124,22
PRIMER_RIGHT_4=392,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=59.317
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_05908 Ps_contig_mira-and-tgicl-ass_franssen_40512
SEQUENCE=CAACAACAACGGGAACAGTAACAACAGTAACATCAACATCGGTGTCGGAAACGGAAAAGCAAGGTATAGAGAGTGTTTAAAGAATCATGCTGTCGGAATCGGTGGTCATGCTTTAGACGGTTGCGGCGAGTTTATGCCGGCGGGGAATGAAGGAAGTTTGGAATCGTTAAAATGTGCCGCGTGTAACTGTCACCGTAATTTCCACCGCAAGGAGTCGCCTGACTTCACCGCCGGGGATCCTTTTCTGTTGACACACCACCACCACCATGCGCTACCTCAGCCGCAGTTTGCGGCTTATTATCGAACTCCGGCTGGGTACTTGCATGTTTCCGGACAGCAGAGGACCGGGACTCTTGCTCTACCGTCGACCTCAGGCGGAGGAGGAGGCGGAGGGACGCAGAGTACGAGGGAGGATCAAGACGAAGACGTTTCGAATCCGAGCGGCGGTGGTTCGAGTAAGAAGCGACACCGAACAAAGTTCACGGTTGAACAGAAGGATAAGATGTTGGAATTAGCTGAGAAATTGGGGTGGAGAATTCAGAAACATGATGAAGGTTTGGTTCAAGAATTCTGCAATGAAAGTGGTGTTAAGCGTCATGTTCTTAAGGTTTGGATGCATAACAACAAACACACTCTTGGTAAGAAGCCCTAGAAAAAATAAAATTAATAATAACAAAAAATTTCCAAAATTTTCTTTCTTAGGGTTTATTAATCCATTAATCCATTGTGGAAGTAGAATGGAACAATATAAATATTTATTTAAGGTACTTAATTTGTGTTAATGTTGTGGAGTTGGAGGGTTAATTAAGTTAAGGTAAGAAGATTTAGTACTAGAAGTAATTAGAGGTTTGTTGATGATGATGATGAAGTTTATTTATGTGTACAATGTAGATGTTTTCTATGGTGAATGAATGCATTTGGATTTGGATTTAGATTTARNAAAAGG
TARGET=254,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05909 Ps_contig_mira-and-tgicl-ass_franssen_40512
SEQUENCE=CAACAACAACGGGAACAGTAACAACAGTAACATCAACATCGGTGTCGGAAACGGAAAAGCAAGGTATAGAGAGTGTTTAAAGAATCATGCTGTCGGAATCGGTGGTCATGCTTTAGACGGTTGCGGCGAGTTTATGCCGGCGGGGAATGAAGGAAGTTTGGAATCGTTAAAATGTGCCGCGTGTAACTGTCACCGTAATTTCCACCGCAAGGAGTCGCCTGACTTCACCGCCGGGGATCCTTTTCTGTTGACACACCACCACCACCATGCGCTACCTCAGCCGCAGTTTGCGGCTTATTATCGAACTCCGGCTGGGTACTTGCATGTTTCCGGACAGCAGAGGACCGGGACTCTTGCTCTACCGTCGACCTCAGGCGGAGGAGGAGGCGGAGGGACGCAGAGTACGAGGGAGGATCAAGACGAAGACGTTTCGAATCCGAGCGGCGGTGGTTCGAGTAAGAAGCGACACCGAACAAAGTTCACGGTTGAACAGAAGGATAAGATGTTGGAATTAGCTGAGAAATTGGGGTGGAGAATTCAGAAACATGATGAAGGTTTGGTTCAAGAATTCTGCAATGAAAGTGGTGTTAAGCGTCATGTTCTTAAGGTTTGGATGCATAACAACAAACACACTCTTGGTAAGAAGCCCTAGAAAAAATAAAATTAATAATAACAAAAAATTTCCAAAATTTTCTTTCTTAGGGTTTATTAATCCATTAATCCATTGTGGAAGTAGAATGGAACAATATAAATATTTATTTAAGGTACTTAATTTGTGTTAATGTTGTGGAGTTGGAGGGTTAATTAAGTTAAGGTAAGAAGATTTAGTACTAGAAGTAATTAGAGGTTTGTTGATGATGATGATGAAGTTTATTTATGTGTACAATGTAGATGTTTTCTATGGTGAATGAATGCATTTGGATTTGGATTTAGATTTARNAAAAGG
TARGET=853,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05910 Ps_contig_mira-and-tgicl-ass_franssen_40514
SEQUENCE=AAGCAGTGGTTCAACGCAGAGTAGCGGGGTAAGGNCNACGTGAACGATGCACCCATGAATAGAAATTCAATTCAACCCTCTTTTTCCCCATTTGAAATCTCTGGTCAAACTAAGAAAATTCACTTGCGTCACAAGAATTACGATTCCACGCACTTCGATTTTGCGCTCACCACCATGAACCTCAACGAAAGATCAAAAGCTCGATTTCTCCCTCCATTAAGAATCAAGAATAAATTGAATCTGTATGGAAATAAAATCAAAACGAATTCGGAACAGAAATTGCAGGTAGAAGAACTCATCGGCTTTACATTCCGATACGGTGGCTCTAGGTCTTGATTTCGATTTTGCTGGTTATGGCTTTTGGTTATGGATCATTGCATTGGAATTGGCTATGAAGCTATGAATTTAGATTAAAATGGTTGTAATGAATTAATTTGGATGTAACTATGTATGTGGTTTATGGTTGTAATTAGATGTAATTGGAAATTGGATTTGTAATAGAAATGATGCATTATGGTTATGATTGTATGGAATTTGAATTCGTAATTGTATGTATGTATGTAATGGTTCATGGTTGANTTNGT
TARGET=548,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05911 Ps_contig_mira-and-tgicl-ass_franssen_40517
SEQUENCE=GTCGGGGAATAAACATTACCTCCAARAAAGCAAGACCCAACACCGAACAAATTGAATTCTATTTTCTCGAGAGATTTAGATCTAAAAACAGTGTTTTCTTCTAAGAAGGTCAAGTTATGAAGTTAAGAGATGGAAGTGAATTCACTCTGCTAGTATTGTTACTGTGCTTAACAACTCTCTCTCTTAAAACAGAAGCTGTGTGGATGACAATTCCCAGTTCAGGAACAAAGTGCGTGTCTGAGGATATCCAAACACACGTTGTCGTTTTAGCTGATTACTATGTTGTCACTGAAGAAGGTCCTCAGCTTCAAACAATTTCCGCCAAGGTAACTTCTCCTTATGGAAACAACCTTCACCACAATGAAAATGTTACACAAGGGCAGTTTGCATTCACAACTACTGAAAGTGGAAATTATGTTGCGTGCTTTTGGATGGGTGGAAATCAACAAGAAGGTGCAACTGCAAGTGTAAACCTTGATTGGAAAACTGGAATTGCAGCCAAGGATTGGGATTCTGTTGCAAAGAAAGAAAAGATTGAGGGTGTTGGACTTGAACTCTTAAAGCTTGAAGGAATAGTG
TARGET=175,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05912 Ps_contig_mira-and-tgicl-ass_franssen_40523
SEQUENCE=AGATCTTTTCTTTTTCTCTTCCTTCTTCTACCTACATAACATACGTCAATACCTCATTCTCATTCTCATTCTCTCTCTCTCTCTCTAGAATGGCGACTCCACCGGTGGTCAGATTTCCGATCTTCGCAACGGTTAGAGCGATTGGAGTTCTCATCATTCTGTTACTCTTAACGTGGGTTCTTCATTTCAGGGGAGGTCTCGCTCTCTTTTCCGATAACAAAGATCTCATCTTCAATGTTCATCCGGTGTTGATGGTGATTGGGCTTGTTCTAATCAATGGTGAAGGGATGCTTGCCTACAAGACAGTATCCGGAACTAAAAACTTCAAAAAAACAGTTCATCTGGCATTACAGTTTCTTGCTCTCGGTTTGAGCATAATTGGTCTCTGGGCTGCTTGGAAGTTTCACAATGACAAGGGCATTGACAATTTCTACAGCCTGCATTCATGGTTAGGCCTTGCATGCCTTCTCCTCTTCTTCATCCAGTTGGCTGCTGGATTTGCAACATTTTGGTATCCAGGAGGGTCAAGAAACAGTAGAGTTGCACTAATGCCGTGGCATGTATTCTTTGGTACCTATATCTATGCTTTGGCTATAGCTACAACAACTACTGGTCTTTTAGAAAAGGCTACATTCCTTCAAACCAACAACGTCATATCGCGATATTCCAATGAAGCAATTCTGGTCAATGTCTTAGGCATTTTGATTGTTGCTTTAGGTGGCTTTGTTATTCTTGGTCTCGTTACGCCGACTTTTAATAAAGCTGATGTTTTAAGAGGAAATGAATAAGTGAGTGATTGAATAATTCAAAATTGAGAACATGAACACACTTGAAAGATGCATTCTCTTAGGCTGTAATGGATTGTTGTATGAACAAACTCTTGAACATATATATACACAAGTTTAGTTTTCTTCTCAGAGATGAATGTTCGTTTCTCTTTTGTCAATGAATGATTCATTTCTTTGTTTGGTGCAT
TARGET=70,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9440
PRIMER_LEFT_PENALTY=2.841343
PRIMER_RIGHT_PENALTY=0.102638
PRIMER_LEFT_SEQUENCE=CGTCAATACCTCATTCTCATTCTC
PRIMER_RIGHT_SEQUENCE=AATGATGAGAACTCCAATCGCT
PRIMER_LEFT=43,24
PRIMER_RIGHT=157,22
PRIMER_LEFT_TM=59.159
PRIMER_RIGHT_TM=60.103
PRIMER_LEFT_GC_PERCENT=41.667
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=3.0000
PRIMER_LEFT_1_PENALTY=2.841343
PRIMER_RIGHT_1_PENALTY=0.158697
PRIMER_LEFT_1_SEQUENCE=CGTCAATACCTCATTCTCATTCTC
PRIMER_RIGHT_1_SEQUENCE=GATTAGAACAAGCCCAATCACC
PRIMER_LEFT_1=43,24
PRIMER_RIGHT_1=274,22
PRIMER_LEFT_1_TM=59.159
PRIMER_RIGHT_1_TM=59.841
PRIMER_LEFT_1_GC_PERCENT=41.667
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=3.1830
PRIMER_LEFT_2_PENALTY=3.080331
PRIMER_RIGHT_2_PENALTY=0.102638
PRIMER_LEFT_2_SEQUENCE=ACGTCAATACCTCATTCTCATTCTC
PRIMER_RIGHT_2_SEQUENCE=AATGATGAGAACTCCAATCGCT
PRIMER_LEFT_2=42,25
PRIMER_RIGHT_2=157,22
PRIMER_LEFT_2_TM=59.920
PRIMER_RIGHT_2_TM=60.103
PRIMER_LEFT_2_GC_PERCENT=40.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=3.2390
PRIMER_LEFT_3_PENALTY=3.080331
PRIMER_RIGHT_3_PENALTY=0.158697
PRIMER_LEFT_3_SEQUENCE=ACGTCAATACCTCATTCTCATTCTC
PRIMER_RIGHT_3_SEQUENCE=GATTAGAACAAGCCCAATCACC
PRIMER_LEFT_3=42,25
PRIMER_RIGHT_3=274,22
PRIMER_LEFT_3_TM=59.920
PRIMER_RIGHT_3_TM=59.841
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=3.3439
PRIMER_LEFT_4_PENALTY=2.841343
PRIMER_RIGHT_4_PENALTY=0.502561
PRIMER_LEFT_4_SEQUENCE=CGTCAATACCTCATTCTCATTCTC
PRIMER_RIGHT_4_SEQUENCE=TTCCGGATACTGTCTTGTAGGC
PRIMER_LEFT_4=43,24
PRIMER_RIGHT_4=314,22
PRIMER_LEFT_4_TM=59.159
PRIMER_RIGHT_4_TM=60.503
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_05913 Ps_contig_mira-and-tgicl-ass_franssen_40531
SEQUENCE=TTTAATANAGAATTTCTTTATAAATTTTTTAACATATATACCAAAGATGCAACTTAACATTACCCTTTAGAAAATATAAACAAATAAAATATAACTAATTAAATATAACTGCATGTCTGATACCAAACACTTCTAGAGATGTCTTTACTCTAAAGTATAACAATATACTAATAACTAAAAGAGCTTAGCGTTGATGCTTATTACTTCACACAAGTTTAACATCTGGGTTTCTCATAGGTTTAAGCCACATATCAGCCATAATTTTCGATGAAATACTCCACAACAAATCTTGCCCTTTCACCAATGCATGTTTTCCTTTCTTCCCTCCTTTTCCTAATCCATATTCAATCAACCATTGCATTGAAGTTAACCTTTGTTTCTCACTATCCCAACCATTCTTCTTTCCATCACCACTCCTCACCTTAAACCCTCCCACATGCATACTACTTACTCTAAACCTCTTCTCCTCCTCCTCCTCATCATCACCCTTTGTGTCCACATTTGTTGCATGAATTAACACCATCACAGGTCCTCCAACTGCTTCATATCTTCTTCACTCTCCAAAAGCCGCCTACCACCATACAAACCTCCAACCACAACGCTCGACGAAAAAACCCTTTNC
TARGET=464,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0815
PRIMER_LEFT_PENALTY=0.049761
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=TTTCCTTTCTTCCCTCCTTTTC
PRIMER_RIGHT_SEQUENCE=GATATGAAGCAGTTGGAGGACC
PRIMER_LEFT=311,22
PRIMER_RIGHT=548,22
PRIMER_LEFT_TM=60.050
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=0.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.0947
PRIMER_LEFT_1_PENALTY=0.062929
PRIMER_RIGHT_1_PENALTY=0.031769
PRIMER_LEFT_1_SEQUENCE=CCTTTCTTCCCTCCTTTTCCTA
PRIMER_RIGHT_1_SEQUENCE=GATATGAAGCAGTTGGAGGACC
PRIMER_LEFT_1=314,22
PRIMER_RIGHT_1=548,22
PRIMER_LEFT_1_TM=60.063
PRIMER_RIGHT_1_TM=59.968
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=235
PRIMER_PAIR_PENALTY_2=0.1202
PRIMER_LEFT_2_PENALTY=0.088469
PRIMER_RIGHT_2_PENALTY=0.031769
PRIMER_LEFT_2_SEQUENCE=TCTTCCCTCCTTTTCCTAATCC
PRIMER_RIGHT_2_SEQUENCE=GATATGAAGCAGTTGGAGGACC
PRIMER_LEFT_2=318,22
PRIMER_RIGHT_2=548,22
PRIMER_LEFT_2_TM=59.912
PRIMER_RIGHT_2_TM=59.968
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.1398
PRIMER_LEFT_3_PENALTY=0.137489
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=AACCCTCCCACATGCATACTAC
PRIMER_RIGHT_3_SEQUENCE=GGCTTTTGGAGAGTGAAGAAGA
PRIMER_LEFT_3=425,22
PRIMER_RIGHT_3=568,22
PRIMER_LEFT_3_TM=60.137
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=0.1419
PRIMER_LEFT_4_PENALTY=0.110131
PRIMER_RIGHT_4_PENALTY=0.031769
PRIMER_LEFT_4_SEQUENCE=CTTCTTTCCATCACCACTCCTC
PRIMER_RIGHT_4_SEQUENCE=GATATGAAGCAGTTGGAGGACC
PRIMER_LEFT_4=397,22
PRIMER_RIGHT_4=548,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.968
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_05914 Ps_contig_mira-and-tgicl-ass_franssen_40539
SEQUENCE=TCGTCGGGGATTGTTCTAACTTTGTCTCATTTTCTCTCTATCTCTCTCTCTCTCATGGCATTTCTCTTCTCCTCTCTCCCTCAGATTAAAATTTAAAAGTCTCTGATTCCATAAAACGACCCCACTTTCCCACTATTGTTATGATAATTTGGAACCTTTTTCACGCAAGAATAAGCTCTACGCACGCAATATTGCTATTGTTTCTTCTTCATATTTTCCTTTTTTTGGTTAAACAAGTTGTTTCTGGGAACCTTGAATATTCAACCTGTGATATGGGCTACCTCAATTCTGTTTTGTCTTCTTCAAGTCAGGTTCATGCTGCTGATGATTCACCTGTTAGTGGTGGAGGACTCAGTCAGAATGGAAAGTTCAGCTATGGATATGCTAGCTCCCCTGGCAAAAGATCTTCAATGGAAGATTTTTACGAGACAAGAATTGACGGCGTTGATGGTCACGGCGGCGCTCGTGCTGCAGAGTATGTCAAGCAAAACCTGTTCAGCAAATTTGATCAGTCACCCAAAATTCATTTCTGA
TARGET=41,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1293
PRIMER_LEFT_PENALTY=0.128591
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=CGGGGATTGTTCTAACTTTGTC
PRIMER_RIGHT_SEQUENCE=CAAAACAGAATTGAGGTAGCCC
PRIMER_LEFT=4,22
PRIMER_RIGHT=295,22
PRIMER_LEFT_TM=59.871
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=10.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=292
PRIMER_PAIR_PENALTY_1=0.1293
PRIMER_LEFT_1_PENALTY=0.128591
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=GTCGGGGATTGTTCTAACTTTG
PRIMER_RIGHT_1_SEQUENCE=CAAAACAGAATTGAGGTAGCCC
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=295,22
PRIMER_LEFT_1_TM=59.871
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=0.1407
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=0.012153
PRIMER_LEFT_2_SEQUENCE=CGGGGATTGTTCTAACTTTGTC
PRIMER_RIGHT_2_SEQUENCE=TTCAAGGTTCCCAGAAACAACT
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=256,22
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=60.012
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=253
PRIMER_PAIR_PENALTY_3=0.1407
PRIMER_LEFT_3_PENALTY=0.128591
PRIMER_RIGHT_3_PENALTY=0.012153
PRIMER_LEFT_3_SEQUENCE=TCGGGGATTGTTCTAACTTTGT
PRIMER_RIGHT_3_SEQUENCE=TTCAAGGTTCCCAGAAACAACT
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=256,22
PRIMER_LEFT_3_TM=59.871
PRIMER_RIGHT_3_TM=60.012
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=254
PRIMER_PAIR_PENALTY_4=0.1407
PRIMER_LEFT_4_PENALTY=0.128591
PRIMER_RIGHT_4_PENALTY=0.012153
PRIMER_LEFT_4_SEQUENCE=GTCGGGGATTGTTCTAACTTTG
PRIMER_RIGHT_4_SEQUENCE=TTCAAGGTTCCCAGAAACAACT
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=256,22
PRIMER_LEFT_4_TM=59.871
PRIMER_RIGHT_4_TM=60.012
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_05915 Ps_contig_mira-and-tgicl-ass_franssen_40542
SEQUENCE=GARTNCATYYNNGGGTAGGGTCTCTAAATTCTGACCATGTTTTACTGAAACACCAATCTCGCAAGAAAACAAGAAAAGTGCGAAACCGAACGAGCGAGCGAGTGCAGAAAACAAGAACAAAAGRCTTCACCAAGGTTTAACCTGTTAACGCTAACAATGTTGTTTCGGTTGCTGAGAACGAAGATAATTCGTTTGATCTTTCATTTCATATCCAAAACGCCGTTAATCCCAGTCGTTTTGCTCTGCTCCACAGATTCATCGCAACAGATTCGATTCTTTTAACCTGTTATTAAGGTCACATGGCTTGGAGGCGTTTGATAACACAGGTTGATAGGCGTCAATCAGAATTTGGAAAGGTCAAAGATATATTGAGTAGAAGCTATTTATCTCGTAATAAGTTTGATGGATGTTCAAGGAATGGGCTTTTCTTCTCCCAGGAAAGATCCCGATCCAGCTATCTTGGAAATCTGGCCCGACGACTGCGTGATTCATCTGAAGCCGAGGAAGCCTCTTATCTAAAAGAACTTTACCGTAGAAATGATCCTGAATCCGTGATTAGAGCATTTGAAAGTCAGCCATCTCTGCATACAAATTCGTCGGCACTTGCTGAATATGTTAAAGCATTGGTAAAAGTTGATAGGCTGGATGAAAGTGAATTACTCAAGACTTTGCGAAGAGGTATTTCCAATTCCGTGAGAGAGGAAGAAAGTAGTATAGGTGGCCTTGCTGCTCTTAGAAATGTGGGTAAAACTACAAAAGATGGAATTCTTGGAACTGCAACTGCTCCTATTCATATGGTTGCTGCATCAGAAGGTGGAAATTTCAAGGAACAGCTATGGCGTACAATTAGATCTGTTGCCGTGGCCTTCCTCTTGATTTCTGGTGTAGGAGCTCTCATTGAGGATAAAGGAATTAGTAAAGGTCTTGGTATGAATGAAGAAGTGCAGCCCAGTGTGGAGACAAGTACTAAATTTAGCGATGTGAAAGGTGTGGATGAGGCAAAGGCTGAACTTGAAGAAATTGTTCATTATCTAAGAGATCCTAAGCGCTTTACTCGTCTTGGTGGCAAACTCCCAAAAGGTGTTTTGCTTGTTGGCCCGCCTGGCACTGGAAAAACAATGTTGGCAAGAGCCATTGCTGGAGAGGCTGGGGTTCCTTTCTTCTCATGCAGTGGAAGTGAATTTGAAGAGATGTTTGTTGGTGTTGGTGCACGAAGGGTGAGAGATCTTTTTACTGCTGCGAAAAAGAGGTCGCCTTGTATAATCTTTATTGATGAGATTGATGCTATTGGCGGAAGTCGAAATCCAAAGGACCAAATGTACATGAAGATGACTTTAAATCAAATGCTTGTTGAGTTAGATGGATTCAAACAAAACGAAGGCATAATTGTAATTGGTGCTACTAATTTTCCTGAGTCACTCGATAAGGCTCTGGTGAGACCTGGGCGTTTTGATCGCCATGTCGTTGTTCCCAATCCAGATGTAGAAGGACGGCGACAGATTATGGAATCTCACATGTCAAAGGTTCTGAAAGCGGATGATGTTGATCTGATGATCATTGCTCGGTGTACACCTGGTTTCTCTGGTGCTGACCTTGCAAATTTGGTTAATGTTGCTGCCCTCAGGGCTGCAATGAATGGCTCCAAAGCCGTGAGCATGCATGATTTAGAGTTTGCACGGGACAAGATCATGATGGGAAGTGAACGTAAGTCAGCTGTTATATCTGAGGAGTCAAGAAAGACTACTGCTTTTCATGAGGGTGGCCATGCCTTGGTAGCAGTTCACACTGATGGTGCGCTTCCTGTTCACAAGGCAACTATAGTTCCTCGCGGGATGGCTCTTGGCATGGTTTCTCAACTGCCGGACAAGGATCAGACTAGTCACTCTCGTAAACAGATGCTTGCTAGGCTTGATGTTTGTATGGGAGGCCGGGTTGCCGAAGAGCTAATATTTGGAGAAAGTGAAGTTACTTCTGGTGCCTCCTCAGATCTTTCACAGGCAACTTCTTTGGCCAGGGAAATGGTTACAAAATATGGCATGAGTACTGAAGTTGGTCCTGTCACTCACAATTACTATGATAATGGGAGAAGTATGAGCTCAGAAACTAGGCTGCTTATTGAGAAAGAAGTGAAGAACTTACTGGAAAGAGCTTACAACAATGCAAAAACTATCCTAACCACCCACGAAAAGGAGCTTCACGCACTTGCAAATGCTCTTCTGGAGCAGGAGACACTTACAGGAAGTCAGATAAATGAATTACTTGCAAAGGTCAAATCCCAGAAGCAACAACCGCAGTCACGCGTAGTTGAAGCTCAGGGAAATTCACGGCCCAATCCAGCAGCTGCTGCGGCTGCCGCGGCCGCTGCAGCTGCAGCTTCTGCTGCTGCAAAGGCTCAAGGAGTTGCCCCAGTAGGATCTTAGCGCAGAAGACTGGCGATGCTAAATAATCACTACCGGATTAATATGTGCAGATAGTAATTTATTCGGTGTTTTTGTGGGTTCCACTTCAGTCCATGGAACTCAACTTGTGTATTCAGGGTCACAGGCTGACCCCATATTTTAGTGAGTTGAAATATCTCGTAGGAGTTCCCATTAATGTTTTTAATAATTGTCTTGTTTACTTTTATATAGAATTTTCCGTCAAAACTGCAATTATATTTTTATCTTTCTCAAACTTTCACGTTACTATTTTTCTCATATTTATT
TARGET=91,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05916 Ps_contig_mira-and-tgicl-ass_franssen_40547
SEQUENCE=TATTGCGTCTGCAAGAGGAGAAAGCTTTGATGGAAATGGAGATGAGACAATACGATAGGTTAATTGAAGAAAGAGTTGCTTATGATGAAGAAGAGATGAGTATTATGCAAGAGATTCTGATAAGAAGGGAAAAGGAGAATCTCTTTCTGGAAAAGGAACTCGAAAGGTATAGGCAAATGTGTTTGACAGGAAATTGTGAGTCGAAATCTAAGTCGGCGGATGTACGGCTTAATGAATGGAAACAACAGTCACCACTTTCGTTTGAAATATATGATGATCCACCGCAGACAGAAAGTGCCGTATCAAATGTTAAGAGAGATTTTACCGATACGGAACATGGAGAAGAGCTGGAGAAGAACACTAGACACAAGGATCAAGCATGCGATGATCTGCATAGCTCTTTCGATGATACCGAGTCCGATGTTCTTGACGTCCATGTTATTGATGACAACATCGAACATAAGGAAAAGGAAATTGAAAATTCAAGCAGCTCTTCATGTAGTACTAGTCTTTCAGACAAACCGATGAATACACGTCTTGAACACGCAAGTTGTTCAGATGCTGACTCAAAATGCAAATCTATACCTTTCGACACTGAAAGTGATTCTCCGTATTTGGTTCATAATGAAAAGTTAAGGATTGACAATGAGATTGAAGTTCTTGGAGAAAGGTTGAGGACAGTGAAGCATGAAAAAGAGAAATTGACTTTGTTTACAGACAAAGGCGAAAACGAAAAAGGTCAGTTGAAGCTTTTGGATGAGATAGCATCCCGTCTTCAACAGATTAAACAATTAAGGAAGCCTGCACGCGACGCATCCTTGCCCCCTAAGCCTGCGCGTGGTGCATCCTTGCCCCCTTCATGGACTCAGGTGAGCGAGAGGAAGAGGCGCAGCCAAAGTGTGACTTTGGAGACTTGTGAAAGCTCCTAAATGATGTAGAGTTTGTTAATTGACATGAGGTTTCATAGCCAAGCATCCTTGTAAACTTGTAAATGCTATGTTGCCACTAGAATTCAGCAGTACTTCCCTCTATCTTGTTGTATGTTTGAGAACTTATAGTTTTTGTAAATAAATAAATGTTATCTGT
TARGET=1065,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05917 Ps_contig_mira-and-tgicl-ass_franssen_40555
SEQUENCE=GGCATTCGGTGGCCGGCTACTAAAGTTCGCATCATGAACCTTTAAGAATTGTTCGGCCACGGTAGCGGAAGCCGCGACGACAACATCGACAAACCCTAATTTGAGGTGCATCAACGGGCCATAGGTTTCAGCCAAGGCTGCAAGAGATTGGTGTGGGGCAGAGCCTAAGTGTGGAATGTTACCTATTATAGGCCATGGTTTTGGTCCTGGTGGAAGTGGGAGTGATGAAGAAGAAGAAGAAGATTTTGTTGCAAATTTTAGAAAACGGTATATTAAGATAGATAATATGAGAGTGGAGAATGCAATAAGCCATAGAGACATGGTTTCTATGTTGTGATTATGAAAATGGGGATGATGATGATAAAGGACAAGAATGTGTTTTGAAAAGAAAGTAGTTGACGTTTATTGTTGTCTC
TARGET=227,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1295
PRIMER_LEFT_PENALTY=0.122569
PRIMER_RIGHT_PENALTY=0.006951
PRIMER_LEFT_SEQUENCE=GACAAACCCTAATTTGAGGTGC
PRIMER_RIGHT_SEQUENCE=ATGGCTTATTGCATTCTCCACT
PRIMER_LEFT=87,22
PRIMER_RIGHT=312,22
PRIMER_LEFT_TM=59.877
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=226
PRIMER_PAIR_PENALTY_1=0.2436
PRIMER_LEFT_1_PENALTY=0.236697
PRIMER_RIGHT_1_PENALTY=0.006951
PRIMER_LEFT_1_SEQUENCE=CAAACCCTAATTTGAGGTGCAT
PRIMER_RIGHT_1_SEQUENCE=ATGGCTTATTGCATTCTCCACT
PRIMER_LEFT_1=89,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=60.237
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=224
PRIMER_PAIR_PENALTY_2=0.2494
PRIMER_LEFT_2_PENALTY=0.122569
PRIMER_RIGHT_2_PENALTY=0.126860
PRIMER_LEFT_2_SEQUENCE=GACAAACCCTAATTTGAGGTGC
PRIMER_RIGHT_2_SEQUENCE=CCTTTATCATCATCATCCCCAT
PRIMER_LEFT_2=87,22
PRIMER_RIGHT_2=366,22
PRIMER_LEFT_2_TM=59.877
PRIMER_RIGHT_2_TM=59.873
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=280
PRIMER_PAIR_PENALTY_3=0.3636
PRIMER_LEFT_3_PENALTY=0.236697
PRIMER_RIGHT_3_PENALTY=0.126860
PRIMER_LEFT_3_SEQUENCE=CAAACCCTAATTTGAGGTGCAT
PRIMER_RIGHT_3_SEQUENCE=CCTTTATCATCATCATCCCCAT
PRIMER_LEFT_3=89,22
PRIMER_RIGHT_3=366,22
PRIMER_LEFT_3_TM=60.237
PRIMER_RIGHT_3_TM=59.873
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=0.3703
PRIMER_LEFT_4_PENALTY=0.122569
PRIMER_RIGHT_4_PENALTY=0.247716
PRIMER_LEFT_4_SEQUENCE=GACAAACCCTAATTTGAGGTGC
PRIMER_RIGHT_4_SEQUENCE=GGCTTATTGCATTCTCCACTCT
PRIMER_LEFT_4=87,22
PRIMER_RIGHT_4=310,22
PRIMER_LEFT_4_TM=59.877
PRIMER_RIGHT_4_TM=59.752
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_05918 Ps_contig_mira-and-tgicl-ass_franssen_40555
SEQUENCE=GGCATTCGGTGGCCGGCTACTAAAGTTCGCATCATGAACCTTTAAGAATTGTTCGGCCACGGTAGCGGAAGCCGCGACGACAACATCGACAAACCCTAATTTGAGGTGCATCAACGGGCCATAGGTTTCAGCCAAGGCTGCAAGAGATTGGTGTGGGGCAGAGCCTAAGTGTGGAATGTTACCTATTATAGGCCATGGTTTTGGTCCTGGTGGAAGTGGGAGTGATGAAGAAGAAGAAGAAGATTTTGTTGCAAATTTTAGAAAACGGTATATTAAGATAGATAATATGAGAGTGGAGAATGCAATAAGCCATAGAGACATGGTTTCTATGTTGTGATTATGAAAATGGGGATGATGATGATAAAGGACAAGAATGTGTTTTGAAAAGAAAGTAGTTGACGTTTATTGTTGTCTC
TARGET=351,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05919 Ps_contig_mira-and-tgicl-ass_franssen_40558
SEQUENCE=AACCATGCAAAAATAAATTTATTGGACATAAAAAGAAATTTAAACACAACCATAGTATAACACAATCTACATCGTGTTCAACAATAATAACATAACATGCAATTATACTAATGGATTAATATCAATATTAGCATAATTATAATCTTAATTTCAGCTTTTAATCACACTGAACCTTGAGAAATCTTGCTCATCACTTTAACGTCTGTGGTGACGGGTGCAGCAACACCTTGGTATGGAGGAGCGTGTACTACCCCACCCGGCGTATTATAATAAACCTGTCTGCCAGTGGCACTATCATACGCCACTTGTGCGTAGGAACCTGGCGCATTATCCGGTATCCCAGTTGATCGAACCACACTGACACCTTCCGCATACGCAGGACCTTTTACCGGTTGCGCCGCTGCATATGTCGCCGACACTTTGGGAGGTGGAGCGCCGCCGTAAACCTGCGCTTCACGGTAACCATCAGATGACATTCGTTGCACCGCGTAGTATCCTTGACTTACCGGTGGACGCATCACCGGTGCATGATAGTAATTTCCCGGCGCTGGTACCATATAAACAGGCTGAGGCTGGTCACCTCCTCCAGGAGCAACCGGTGGATAAGCCTCCCCAGAAACAGGCTTTTCCTGCCAATAACCAGTGTGCTGCTGCTGCTGCTGCGGTGGCGCGAAGTTCGACATTGGAAATTTCTCAGGCGTTGTCTTCTGAACGTAATAATCTCCGGGAGGATAATTTCCGACGAGACCATCTTCACTTTTCCTCCGATACGCATTTTGCTCGTTTTCCGCTATCTGCATCCGTTGTAACTGCCTCTGAATATCCAACGGGTGGTTCAAACCCGGATCCGAAACAATAACCCGATCAGACGGATTCAAATTCAAACCCGGTCGCGTAAGATACTCAGGTGGTGGAGCAACCATTTCCGGCACGGGATCGTTAAATTTAACGACGGGCGGAGGCGGAGCAACGACGGCGACAGGCTTTTCAATTCCAAACATATAATCAACGTTGGAAGGTTTAATCGGGTCGGGTGGGTTTGAATTTGAAGCAGAATCAGAATCGTTAACAAAGAGGAATAATCGCATACGTGCGGGTTTAGCGGAAGCACGGTAGAGACGATCGTATTCATGCATCATGTGGTCGAGATCATCGTCGTTGGTGACAGAGATAAGAGCATCGAGATCCTCGCCGGGGAGTTGGTACTTAAAAAGAAACATCAGGTGATTCTATGAGAGAACAGAGTTTAGAAATGATTGAAGAGAATTTGATGTTGCGATCAACGGCTAGGATTTTGGTTTCGCCACCAACGTAAGAGAGTTGGTTGTCGTGTGTTCGTGGTTGGATCTTGCCACCGTAGCTACACATGAACTTGGCTTTGTAGTTACTAGTTTGTTGTTGTTGATGATGTTGTTGTTCGTTCCATGGGATTGGTGTGGGTGGGTTTTCGAAGTCGATTTCTCTAGAACGAGGTGAAGATTCTCCTGAATCTGGGTATGATTGATACGAATAGTTCTCCATTTGGATTTTCGAACACTTCAATTTTCCTTAATTTGGTTTGTCCCACTCAGTAGTGTCGTGTGTGTATCCTTATGTTGTTGTTATTTGATGTATGTTAATGTTATGTGATCATGAGTGCATGACTCGTGACTTCATGGAAANTACATA
TARGET=646,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2756
PRIMER_LEFT_PENALTY=0.241544
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=GTATCCTTGACTTACCGGTGGA
PRIMER_RIGHT_SEQUENCE=AAGATGGTCTCGTCGGAAATTA
PRIMER_LEFT=491,22
PRIMER_RIGHT=753,22
PRIMER_LEFT_TM=60.242
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.2756
PRIMER_LEFT_1_PENALTY=0.241544
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=TATCCTTGACTTACCGGTGGAC
PRIMER_RIGHT_1_SEQUENCE=AAGATGGTCTCGTCGGAAATTA
PRIMER_LEFT_1=492,22
PRIMER_RIGHT_1=753,22
PRIMER_LEFT_1_TM=60.242
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.4652
PRIMER_LEFT_2_PENALTY=0.140832
PRIMER_RIGHT_2_PENALTY=0.324355
PRIMER_LEFT_2_SEQUENCE=TTCACGGTAACCATCAGATGAC
PRIMER_RIGHT_2_SEQUENCE=TCCCGGAGATTATTACGTTCAG
PRIMER_LEFT_2=452,22
PRIMER_RIGHT_2=728,22
PRIMER_LEFT_2_TM=59.859
PRIMER_RIGHT_2_TM=60.324
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.4652
PRIMER_LEFT_3_PENALTY=0.140832
PRIMER_RIGHT_3_PENALTY=0.324355
PRIMER_LEFT_3_SEQUENCE=TTCACGGTAACCATCAGATGAC
PRIMER_RIGHT_3_SEQUENCE=CCCGGAGATTATTACGTTCAGA
PRIMER_LEFT_3=452,22
PRIMER_RIGHT_3=727,22
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=60.324
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=0.4652
PRIMER_LEFT_4_PENALTY=0.140832
PRIMER_RIGHT_4_PENALTY=0.324355
PRIMER_LEFT_4_SEQUENCE=TTCACGGTAACCATCAGATGAC
PRIMER_RIGHT_4_SEQUENCE=CTCCCGGAGATTATTACGTTCA
PRIMER_LEFT_4=452,22
PRIMER_RIGHT_4=729,22
PRIMER_LEFT_4_TM=59.859
PRIMER_RIGHT_4_TM=60.324
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=278
=
PRIMER_SEQUENCE_ID=SSR_05920 Ps_contig_mira-and-tgicl-ass_franssen_40558
SEQUENCE=AACCATGCAAAAATAAATTTATTGGACATAAAAAGAAATTTAAACACAACCATAGTATAACACAATCTACATCGTGTTCAACAATAATAACATAACATGCAATTATACTAATGGATTAATATCAATATTAGCATAATTATAATCTTAATTTCAGCTTTTAATCACACTGAACCTTGAGAAATCTTGCTCATCACTTTAACGTCTGTGGTGACGGGTGCAGCAACACCTTGGTATGGAGGAGCGTGTACTACCCCACCCGGCGTATTATAATAAACCTGTCTGCCAGTGGCACTATCATACGCCACTTGTGCGTAGGAACCTGGCGCATTATCCGGTATCCCAGTTGATCGAACCACACTGACACCTTCCGCATACGCAGGACCTTTTACCGGTTGCGCCGCTGCATATGTCGCCGACACTTTGGGAGGTGGAGCGCCGCCGTAAACCTGCGCTTCACGGTAACCATCAGATGACATTCGTTGCACCGCGTAGTATCCTTGACTTACCGGTGGACGCATCACCGGTGCATGATAGTAATTTCCCGGCGCTGGTACCATATAAACAGGCTGAGGCTGGTCACCTCCTCCAGGAGCAACCGGTGGATAAGCCTCCCCAGAAACAGGCTTTTCCTGCCAATAACCAGTGTGCTGCTGCTGCTGCTGCGGTGGCGCGAAGTTCGACATTGGAAATTTCTCAGGCGTTGTCTTCTGAACGTAATAATCTCCGGGAGGATAATTTCCGACGAGACCATCTTCACTTTTCCTCCGATACGCATTTTGCTCGTTTTCCGCTATCTGCATCCGTTGTAACTGCCTCTGAATATCCAACGGGTGGTTCAAACCCGGATCCGAAACAATAACCCGATCAGACGGATTCAAATTCAAACCCGGTCGCGTAAGATACTCAGGTGGTGGAGCAACCATTTCCGGCACGGGATCGTTAAATTTAACGACGGGCGGAGGCGGAGCAACGACGGCGACAGGCTTTTCAATTCCAAACATATAATCAACGTTGGAAGGTTTAATCGGGTCGGGTGGGTTTGAATTTGAAGCAGAATCAGAATCGTTAACAAAGAGGAATAATCGCATACGTGCGGGTTTAGCGGAAGCACGGTAGAGACGATCGTATTCATGCATCATGTGGTCGAGATCATCGTCGTTGGTGACAGAGATAAGAGCATCGAGATCCTCGCCGGGGAGTTGGTACTTAAAAAGAAACATCAGGTGATTCTATGAGAGAACAGAGTTTAGAAATGATTGAAGAGAATTTGATGTTGCGATCAACGGCTAGGATTTTGGTTTCGCCACCAACGTAAGAGAGTTGGTTGTCGTGTGTTCGTGGTTGGATCTTGCCACCGTAGCTACACATGAACTTGGCTTTGTAGTTACTAGTTTGTTGTTGTTGATGATGTTGTTGTTCGTTCCATGGGATTGGTGTGGGTGGGTTTTCGAAGTCGATTTCTCTAGAACGAGGTGAAGATTCTCCTGAATCTGGGTATGATTGATACGAATAGTTCTCCATTTGGATTTTCGAACACTTCAATTTTCCTTAATTTGGTTTGTCCCACTCAGTAGTGTCGTGTGTGTATCCTTATGTTGTTGTTATTTGATGTATGTTAATGTTATGTGATCATGAGTGCATGACTCGTGACTTCATGGAAANTACATA
TARGET=1393,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2306
PRIMER_LEFT_PENALTY=0.164595
PRIMER_RIGHT_PENALTY=0.065960
PRIMER_LEFT_SEQUENCE=CGTAAGAGAGTTGGTTGTCGTG
PRIMER_RIGHT_SEQUENCE=CACTACTGAGTGGGACAAACCA
PRIMER_LEFT=1310,22
PRIMER_RIGHT=1575,22
PRIMER_LEFT_TM=59.835
PRIMER_RIGHT_TM=60.066
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=266
PRIMER_PAIR_PENALTY_1=0.2314
PRIMER_LEFT_1_PENALTY=0.165486
PRIMER_RIGHT_1_PENALTY=0.065960
PRIMER_LEFT_1_SEQUENCE=CGTAGCTACACATGAACTTGGC
PRIMER_RIGHT_1_SEQUENCE=CACTACTGAGTGGGACAAACCA
PRIMER_LEFT_1=1355,22
PRIMER_RIGHT_1=1575,22
PRIMER_LEFT_1_TM=59.835
PRIMER_RIGHT_1_TM=60.066
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.2533
PRIMER_LEFT_2_PENALTY=0.164595
PRIMER_RIGHT_2_PENALTY=0.088662
PRIMER_LEFT_2_SEQUENCE=CGTAAGAGAGTTGGTTGTCGTG
PRIMER_RIGHT_2_SEQUENCE=GTGGGACAAACCAAATTAAGGA
PRIMER_LEFT_2=1310,22
PRIMER_RIGHT_2=1566,22
PRIMER_LEFT_2_TM=59.835
PRIMER_RIGHT_2_TM=60.089
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.2541
PRIMER_LEFT_3_PENALTY=0.165486
PRIMER_RIGHT_3_PENALTY=0.088662
PRIMER_LEFT_3_SEQUENCE=CGTAGCTACACATGAACTTGGC
PRIMER_RIGHT_3_SEQUENCE=GTGGGACAAACCAAATTAAGGA
PRIMER_LEFT_3=1355,22
PRIMER_RIGHT_3=1566,22
PRIMER_LEFT_3_TM=59.835
PRIMER_RIGHT_3_TM=60.089
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=212
PRIMER_PAIR_PENALTY_4=0.3719
PRIMER_LEFT_4_PENALTY=0.164595
PRIMER_RIGHT_4_PENALTY=0.207335
PRIMER_LEFT_4_SEQUENCE=CGTAAGAGAGTTGGTTGTCGTG
PRIMER_RIGHT_4_SEQUENCE=CGACACTACTGAGTGGGACAAA
PRIMER_LEFT_4=1310,22
PRIMER_RIGHT_4=1578,22
PRIMER_LEFT_4_TM=59.835
PRIMER_RIGHT_4_TM=60.207
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_05921 Ps_contig_mira-and-tgicl-ass_franssen_40559
SEQUENCE=AGAAGAAGAAGAAGAAGAAGAGAGTCAAGCGTCGAAGAGTTCTAAAGTTTATCTGATTCTCAGACGTTCGTCCCATCTTTATCTCTGACATTCTCGAGCTTGGTCGGAGCAATTCCCGATTCCGAAGGTTAGTGAACTTCTTTGGACTTCTCTTTTTCTTGTTTCCAGTTGATAGAATTGAGGTAGATAGGCTTTAACACCAAGATTAGGTAACGAACCGAGCCGTGGGAGAGGTTTAATTTCAGTTTTTCTTGTTCTAGGGTTCTGGCTTTCGTTCTTCAATTCAAATTATTGGCTGAGAATTGATAAAAGCGAAGAGTTGGTTTAGAGAGAGGTTGTCAAGACGGATGCTTTGGGTGGTCGAAATGGGCGTTCGGCCGCTCCGCCGCCTTCGGCGTGGCGGAGCCCCCGTTTCCGGTGAGGTTCTGATGACTGAGAGGAGAGAGTTTGGATGTGTATCATTCATTGCACATGTGTCCCTTTGTTAATCATCATTAATTAGCACATGCTTAAAAAAAAACCTCTGACTGCGCACGCTTGAGCTACCGGATATGTGATAT
TARGET=1,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05922 Ps_contig_mira-and-tgicl-ass_franssen_40563
SEQUENCE=AAAGACAAGTAGGTATCAACGCAGAGTACTGGGGATTCAAACATCTCTTCTTCTTTCCCTCACTCTACCCTCTCCTCTAAAAAAAATTCACTTTTTAATAGAATAATATTTTCAAGATTAATTTGTGCATCCTCTTTCTAGTTTTCAAGAACATTGTTTTTGATAGTTCCTTTCCAAATATGCACCTAGAGAGAGAGAAGAAACAATAGCAAGGATCAATCTTCTATTTGNGATTATAGCTTAATTAGAAAAGAAAGTATTTAAGGTAAGTTAAGTTTTTGGCTTGA
TARGET=188,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05923 Ps_contig_mira-and-tgicl-ass_franssen_40572
SEQUENCE=CCNACAGCCATTGTCACAAGAAAATCCCCAAAACAACAACAACAAGAACCAATCGATGAGCTTCGCACCGTGAACAACTACCACCACTCGAGGACGATGATGAGGTCGACCCCTCATGGAACAAAATCTAAATCTCACATTGCTGACAACTGCGACTGATCCAGAAGATGTTAGAGTGATTCCGTGGGAGGATTTTGAGCAGGATCTTGCTAGGTTATCGAGTCTTTCTTCTGCACTCAACGAAGCTAAGGAGAAGAAGCGAAACCTTCAGCACAAACTCGAGTCCCTCATCCAGGTTAATGGTGAATCATTGGGTCGATTGAATGAACTTGAAGAAATGCGTCAAAAATTGGAATCAAAGAAATTAATGATGGATAATATGTCTATACGTTCTAGGCTGGCTAAGGAGGATGCTGGTAAGCAAGAGGAGCAGCTTAGTGGTGCCTTGCAGTCACTTTTGGTGGCGGGTGGTACTCTCTCTGTTACTAGAAGAAACCTTCAGGAATCAAGTAGATTACTCTCCGAAGAAAATGGCTATGTTCGTCTGAGAAACTTGCAAAAGATGCTAAGGATGAGGCAACAAATAACAATAAGAAACAAAATCTCAAATTTAATGCTGTACCCTGTAAAATT
TARGET=32,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5284
PRIMER_LEFT_PENALTY=0.360320
PRIMER_RIGHT_PENALTY=0.168114
PRIMER_LEFT_SEQUENCE=AGCCATTGTCACAAGAAAATCC
PRIMER_RIGHT_SEQUENCE=TTCGTTGAGTGCAGAAGAAAGA
PRIMER_LEFT=5,22
PRIMER_RIGHT=243,22
PRIMER_LEFT_TM=60.360
PRIMER_RIGHT_TM=60.168
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.5319
PRIMER_LEFT_1_PENALTY=0.360320
PRIMER_RIGHT_1_PENALTY=0.171564
PRIMER_LEFT_1_SEQUENCE=AGCCATTGTCACAAGAAAATCC
PRIMER_RIGHT_1_SEQUENCE=CTTAGCTTCGTTGAGTGCAGAA
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=249,22
PRIMER_LEFT_1_TM=60.360
PRIMER_RIGHT_1_TM=59.828
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=245
PRIMER_PAIR_PENALTY_2=0.5319
PRIMER_LEFT_2_PENALTY=0.360320
PRIMER_RIGHT_2_PENALTY=0.171564
PRIMER_LEFT_2_SEQUENCE=AGCCATTGTCACAAGAAAATCC
PRIMER_RIGHT_2_SEQUENCE=TTAGCTTCGTTGAGTGCAGAAG
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=248,22
PRIMER_LEFT_2_TM=60.360
PRIMER_RIGHT_2_TM=59.828
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.5337
PRIMER_LEFT_3_PENALTY=0.360320
PRIMER_RIGHT_3_PENALTY=0.173354
PRIMER_LEFT_3_SEQUENCE=AGCCATTGTCACAAGAAAATCC
PRIMER_RIGHT_3_SEQUENCE=CATTAACCTGGATGAGGGACTC
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=301,22
PRIMER_LEFT_3_TM=60.360
PRIMER_RIGHT_3_TM=59.827
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.5371
PRIMER_LEFT_4_PENALTY=0.360320
PRIMER_RIGHT_4_PENALTY=0.176730
PRIMER_LEFT_4_SEQUENCE=AGCCATTGTCACAAGAAAATCC
PRIMER_RIGHT_4_SEQUENCE=TTAGATTTTGTTCCATGAGGGG
PRIMER_LEFT_4=5,22
PRIMER_RIGHT_4=130,22
PRIMER_LEFT_4_TM=60.360
PRIMER_RIGHT_4_TM=60.177
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=10.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_05924 Ps_contig_mira-and-tgicl-ass_franssen_40576
SEQUENCE=AAGCAGTGTATCAACGCAGAGTCAGAACAAAGCTGAAGCAAACAGAGGTGGATTGTGAGTTCCTAAAGAAATGTTGTGAAACATTAACAGATGAAAACAGAAGGCTACAAAAAGAGGTTCAAGAACTAAAGGCACTGAAACAAGCACAACCTTTGTATATGCCTATGCCTGCTGCAACCCTTACTATGTGCCCTTCTTGTGAGAGGCTAGGTGGTGGTGTTAATGGTGGTTCTTCCAATAAGGGCAATTTTTCAATGGCTCCTAAACCTCACTTTTACAATCCCTTCACAAATTCTTCTGCAGCATGTTGATTTGTTACTATGAAAAGGGATAAAAGAAAAAGAAAAGAAGAAAACAACAAGAGTACTTTCCCACACAACCTTTGTATTTTTGGTTGAGGGAAATGTTTGTGAACTACAGCTAGCTTCTGAAAGGTTAAAGGGTGTAATTAATTAATACTGTAGAAATTAGTAATTGATTATGATTAATTTTAAAAACTTTTT
TARGET=446,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05925 Ps_contig_mira-and-tgicl-ass_franssen_40594
SEQUENCE=CTTGTTGTCCGCAGATGATTACTGATGATGATGATCTGATATTGNAGGCCCTATATTAAGATGAAAATTATGTAGGTTGTAGCATGATGTATGATTTGGTAAGCTCATGCCATGGTTCGCTTGCATTGCATGCTTGTATTGGATTCATGGATGCATGTATTGATTATAATGAGTGCATGGCTGCTGGTTTCTGGCATTGTACATGATGGAAAGGCAAAGCTTGGCATGAGCAACAAAGCCTGACAAAGTTCAAGGAAAGACAAATCAAATGGATGCAAAATCAGATTTCTTGCTCATGAAGAAACATAATGATGGAAACTCAAGAAATGATGCAAGAATGAGAATGTAATTAGTATTAGGGTTTTAAGGATGGAAAAATGTGATAATTGACTTTGGTTAACTGGTTGACCAAAAAGTCAACTTTTGAA
TARGET=23,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4079
PRIMER_LEFT_PENALTY=0.362762
PRIMER_RIGHT_PENALTY=0.045112
PRIMER_LEFT_SEQUENCE=CTTGTTGTCCGCAGATGATTAC
PRIMER_RIGHT_SEQUENCE=CATGCATCCATGAATCCAATAC
PRIMER_LEFT=0,22
PRIMER_RIGHT=156,22
PRIMER_LEFT_TM=59.637
PRIMER_RIGHT_TM=60.045
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.4079
PRIMER_LEFT_1_PENALTY=0.362762
PRIMER_RIGHT_1_PENALTY=0.045112
PRIMER_LEFT_1_SEQUENCE=TTGTTGTCCGCAGATGATTACT
PRIMER_RIGHT_1_SEQUENCE=CATGCATCCATGAATCCAATAC
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=156,22
PRIMER_LEFT_1_TM=59.637
PRIMER_RIGHT_1_TM=60.045
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.6375
PRIMER_LEFT_2_PENALTY=0.362762
PRIMER_RIGHT_2_PENALTY=0.274768
PRIMER_LEFT_2_SEQUENCE=CTTGTTGTCCGCAGATGATTAC
PRIMER_RIGHT_2_SEQUENCE=TCCTTGAACTTTGTCAGGCTTT
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=255,22
PRIMER_LEFT_2_TM=59.637
PRIMER_RIGHT_2_TM=60.275
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=256
PRIMER_PAIR_PENALTY_3=0.6375
PRIMER_LEFT_3_PENALTY=0.362762
PRIMER_RIGHT_3_PENALTY=0.274768
PRIMER_LEFT_3_SEQUENCE=TTGTTGTCCGCAGATGATTACT
PRIMER_RIGHT_3_SEQUENCE=TCCTTGAACTTTGTCAGGCTTT
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=59.637
PRIMER_RIGHT_3_TM=60.275
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=255
PRIMER_PAIR_PENALTY_4=0.6375
PRIMER_LEFT_4_PENALTY=0.362762
PRIMER_RIGHT_4_PENALTY=0.274768
PRIMER_LEFT_4_SEQUENCE=CTTGTTGTCCGCAGATGATTAC
PRIMER_RIGHT_4_SEQUENCE=TTCCTTGAACTTTGTCAGGCTT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=256,22
PRIMER_LEFT_4_TM=59.637
PRIMER_RIGHT_4_TM=60.275
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_05926 Ps_contig_mira-and-tgicl-ass_franssen_40599
SEQUENCE=CTGATACTGGTGCACTTGTCTCTCTATTTTCTAGGCTAGCTAATGRGAAAACATTGTCCCAAAAACTGGAAGATGCACGGAGTGCTGTGCAACTAAGAATTGTGAAAGCCCTCAAGGAGTATCGAAATCTTTATGCTGTGCAACATCGCTTGGCCAACAGAATGATATATCCCGAATCTTTGAAGTTCCTAATGTTGTATGGATTAGCTCTTTGTAGATCAATGGCTCTACGTGGAGGGTATGGTGATGTTCCATTGGATGATCGATGTGCAGCAGGACATACAATCATGATTCTACCAATAAAAAGATTGTTGAAACTTCTCTATCCTAGCTTGATTCGACTTGATGAATATCTTCTAAAGCCATCTGTACAAGCTGATGACTTAAAAAGTATCGAGAGAAGGTTACCTTTGGCTGGGGAGAGCTTAGATTCTAGGGGCCTTTATTTATATGATGATGGCTTCCGGTTTATTATATGGTTTGGTAGGGTGATTTCCCCTGATATAGCAAAGAATTTACTCGGGGCAGACTTTGCGGCAGAATTATCAAAGGCTACTCTCAATGAGCATAATAATGAAATGTCAAGGAGGCTGATGAGGGTACTTGAAAAGTTAAGAAATGATGATCATGGATATTACCAGTTGTGCCATCTTGTGAGGCAAGGTGAACAACCCAAAGAAGGGTTCCTTCTTCTTGCAAACCTTGTTGAGGACCAGATGGGTGGTAATAGTGGGTATGCTGATTGGATGCTACAGATATCCCGACAAGTGCATCACTCATAATATTTAGGTATGAATTGTAATACTGTGAATACCTTGTTGCATGTTTGACTAAATTACTGTGGTAAATTTCTTTAGGGTATGTTTGGGAGGGGAGGTCTTAATTTTCTTTTCTTTTCTAAATTAGCAGGTGAGGGGAGGTCTTAATTTTCCTTTCTAAATTAAGCAGTGTTGTTAATAACACTGAGATTAAGCATGCTATAAATGTTTTNAAATAATGAGCTTGATCAATTAAGT
TARGET=884,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05927 Ps_contig_mira-and-tgicl-ass_franssen_40617
SEQUENCE=GTGGAATGACTAGGATCAGAGTTAGTTAGTTTCCACTCACTGAGTTGAATCCCTAACTCAGCTAGTTAGTAACAACTAAATTAGTTAGTTGTTAGTTGTCCAATAATGAAATCTGATGGTTGCGAAGTGTGAGTTAGTTTCAGACATTTGATTAGGTTAGTGTTAAAACTGAGTTTTTATTCCCTATTATTCAAAATGAGTTGTTAAGTGGATTGCTCGATTTGTGAAATTGCCCTACTATGATTGATTGTTTGCATTGCCTTAAGTCTGCTACAGGACAACATGGAATGACGTGTTGCTCATGGTCTGCATACATAGCTAGGCTTGTGATAACAGAAGTGAATTCCTACAGGTGTAGTTCTACATGATGGTCTATTGCAGGGTTTGCATTTGGCTTTTGCTAGCAAGACTAGGCTTTGTTTTTTACTCATGTTTCATGGTAAGCTGACATGGTTCTGCAGATTTTTGCAAGGTGCAATGCTTTATCAATTGTAGATTAGTATTGGCCTATGGTGTAACATGACCNATACGGGTTCTGCAAGTTTTCATTTTGATCCTTGTATGGTGCTGCTTTGGCTTATGTCCATGTCGTGCATGTGTATTCTGCAGGTGTGGTTCTGACTTGCTTTTGCAGCATGCCATTGCAGAATGATTCATGGTTTTACTGCATTGTGGTTTGTGGCTTACTGACTCAAACTTATGGTTTGCAACATCATGATATGGCATTGCATATTGTTGTTGCAAGTCAGGTTTGTATTGTGCATCATGCTCTGAACATGGTATGCAACAGGTCATGTGCAACTTGGTGTAGCTTGGCATGTTCTACAATGTTGTTGCAAGGTCACATGCACATGGAGGTTGGTTAAACAAGGCAACATGACATCAAAGTTGGAATTTTGAAGCAACATGCATAAATGCAAACCAAAGGACCATGATGGATT
TARGET=20,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05928 Ps_contig_mira-and-tgicl-ass_franssen_40619
SEQUENCE=CATGTTGTCACAAACGATGCCAGATTCAACCTAGGATTTCCATCCAGCATCAGTTCATCGTTTATGATCTGATATGCTGCATCCTTTGGTATCGATTCGTCCGGCATCTTGAATCTAGGAAGTGAAGTTCTGACGTAGCGAGAAGCAAAGGTTGAGTGGACGGAGACATCGGACTCGGAGGCTGTTTTTGAGAGAACCATGGTGGTGACAAAGAAAGAGCTGTTTGTTTGTTTGTTTAAATGTGTTGTTCGGATTCACTC
TARGET=221,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2514
PRIMER_LEFT_PENALTY=0.212766
PRIMER_RIGHT_PENALTY=1.038666
PRIMER_LEFT_SEQUENCE=AAGTTCTGACGTAGCGAGAAGC
PRIMER_RIGHT_SEQUENCE=GAGTGAATCCGAACAACACATT
PRIMER_LEFT=124,22
PRIMER_RIGHT=259,22
PRIMER_LEFT_TM=60.213
PRIMER_RIGHT_TM=58.961
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=1.2920
PRIMER_LEFT_1_PENALTY=0.253332
PRIMER_RIGHT_1_PENALTY=1.038666
PRIMER_LEFT_1_SEQUENCE=CGGCATCTTGAATCTAGGAAGT
PRIMER_RIGHT_1_SEQUENCE=GAGTGAATCCGAACAACACATT
PRIMER_LEFT_1=101,22
PRIMER_RIGHT_1=259,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=58.961
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=1.3276
PRIMER_LEFT_2_PENALTY=0.288917
PRIMER_RIGHT_2_PENALTY=1.038666
PRIMER_LEFT_2_SEQUENCE=GAGAAGCAAAGGTTGAGTGGAC
PRIMER_RIGHT_2_SEQUENCE=GAGTGAATCCGAACAACACATT
PRIMER_LEFT_2=139,22
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=60.289
PRIMER_RIGHT_2_TM=58.961
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=121
PRIMER_PAIR_PENALTY_3=1.3422
PRIMER_LEFT_3_PENALTY=0.303563
PRIMER_RIGHT_3_PENALTY=1.038666
PRIMER_LEFT_3_SEQUENCE=TGATCTGATATGCTGCATCCTT
PRIMER_RIGHT_3_SEQUENCE=GAGTGAATCCGAACAACACATT
PRIMER_LEFT_3=64,22
PRIMER_RIGHT_3=259,22
PRIMER_LEFT_3_TM=59.696
PRIMER_RIGHT_3_TM=58.961
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=1.3536
PRIMER_LEFT_4_PENALTY=0.314939
PRIMER_RIGHT_4_PENALTY=1.038666
PRIMER_LEFT_4_SEQUENCE=GTGAAGTTCTGACGTAGCGAGA
PRIMER_RIGHT_4_SEQUENCE=GAGTGAATCCGAACAACACATT
PRIMER_LEFT_4=121,22
PRIMER_RIGHT_4=259,22
PRIMER_LEFT_4_TM=59.685
PRIMER_RIGHT_4_TM=58.961
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=139
=
PRIMER_SEQUENCE_ID=SSR_05929 Ps_contig_mira-and-tgicl-ass_franssen_40621
SEQUENCE=GGTTTAAGTTGTAACTATANTCAAGAACAAAACTACAATTCTAATAGATATTCACATTCATTACTTTTCATTCCATTCTTGTTGTTGCTATACAAACATGTTACTCATTCTAGCATGGTTTTGCAGCTAGAATGCATCATTTGTTGATGGAAGAGTAATCTCGGTTCAAACCTCAGTTGAATTTCCATTAATTCCCATTTACCAGGAACCATCCATGAGGAACATTCAACATTTATAGAGGAAGACAATTAATAGTCATTATTAAACTTTTGGTGACTCCGTGATGCTTTGTAAAGAAGGGCGCCAAGATTTTGACCTCTGCACAGCAGCAGGATTTGTAGACCCTCCTCTTTGCTTGTGTCTCTCTGCTCTGCCTCCCTGATATGTGTTTCCTTGGTCAGATTCCTCTCCTTTCTTTGTCTCATAACCTGGCAGAACTGCATCTTCCTGTGTCTTCTTACTCTTTGCATGCTTCTTTGCCTTCAACACCTTCATAACCTCTTGATTAGTAATGAGCCTATAAGCATGACCAAGATTAAGAGTCTCATTAGGCCTAAGCAACTTAACACGAGTAAACTTCACAGTCTTTTGTTCTTGATTCTGCTCTTCTGGTAGAACAGGTAATGGAATAATCAAAGAAACATAGTGACCTGGGTTTGTTTTCATAACCTCACTAGCAGTAACTGGCCAATACAATCTATCTATCTTCCCACAAGGATGTTGAATCACAAGAACAGCAGCATCCACAGCTTGACAATTTCCCATGGCAAAGATAAGAGAAAATGGATGAATGAATGAATGAATGAAGGAAAGAAAAGTCTCTATCTATACAGCTTGTAGTAGCACCCCACAAATATAGGAAAATCAGGTTGGAAGAGGTAGTCTTTGTTTTTGTTCTCTAATAATAATAACAAGGCTTCTCTTCACAACAACTAAATGTCCCGCGTACTCTGCGTTG
TARGET=696,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3025
PRIMER_LEFT_PENALTY=0.226971
PRIMER_RIGHT_PENALTY=0.075553
PRIMER_LEFT_SEQUENCE=AGTGACCTGGGTTTGTTTTCAT
PRIMER_RIGHT_SEQUENCE=TTTTCCTATATTTGTGGGGTGC
PRIMER_LEFT=644,22
PRIMER_RIGHT=863,22
PRIMER_LEFT_TM=59.773
PRIMER_RIGHT_TM=60.076
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.3289
PRIMER_LEFT_1_PENALTY=0.226971
PRIMER_RIGHT_1_PENALTY=0.101896
PRIMER_LEFT_1_SEQUENCE=AGTGACCTGGGTTTGTTTTCAT
PRIMER_RIGHT_1_SEQUENCE=CGGGACATTTAGTTGTTGTGAA
PRIMER_LEFT_1=644,22
PRIMER_RIGHT_1=943,22
PRIMER_LEFT_1_TM=59.773
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.3385
PRIMER_LEFT_2_PENALTY=0.226971
PRIMER_RIGHT_2_PENALTY=0.111534
PRIMER_LEFT_2_SEQUENCE=AGTGACCTGGGTTTGTTTTCAT
PRIMER_RIGHT_2_SEQUENCE=GATGCTGCTGTTCTTGTGATTC
PRIMER_LEFT_2=644,22
PRIMER_RIGHT_2=743,22
PRIMER_LEFT_2_TM=59.773
PRIMER_RIGHT_2_TM=59.888
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=100
PRIMER_PAIR_PENALTY_3=0.3478
PRIMER_LEFT_3_PENALTY=0.274768
PRIMER_RIGHT_3_PENALTY=0.073038
PRIMER_LEFT_3_SEQUENCE=CTTCTTTGCCTTCAACACCTTC
PRIMER_RIGHT_3_SEQUENCE=GCTGCTGTTCTTGTGATTCAAC
PRIMER_LEFT_3=471,22
PRIMER_RIGHT_3=740,22
PRIMER_LEFT_3_TM=60.275
PRIMER_RIGHT_3_TM=59.927
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.3810
PRIMER_LEFT_4_PENALTY=0.307953
PRIMER_RIGHT_4_PENALTY=0.073038
PRIMER_LEFT_4_SEQUENCE=AGGCCTAAGCAACTTAACACGA
PRIMER_RIGHT_4_SEQUENCE=GCTGCTGTTCTTGTGATTCAAC
PRIMER_LEFT_4=549,22
PRIMER_RIGHT_4=740,22
PRIMER_LEFT_4_TM=60.308
PRIMER_RIGHT_4_TM=59.927
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=192
=
PRIMER_SEQUENCE_ID=SSR_05930 Ps_contig_mira-and-tgicl-ass_franssen_40621
SEQUENCE=GGTTTAAGTTGTAACTATANTCAAGAACAAAACTACAATTCTAATAGATATTCACATTCATTACTTTTCATTCCATTCTTGTTGTTGCTATACAAACATGTTACTCATTCTAGCATGGTTTTGCAGCTAGAATGCATCATTTGTTGATGGAAGAGTAATCTCGGTTCAAACCTCAGTTGAATTTCCATTAATTCCCATTTACCAGGAACCATCCATGAGGAACATTCAACATTTATAGAGGAAGACAATTAATAGTCATTATTAAACTTTTGGTGACTCCGTGATGCTTTGTAAAGAAGGGCGCCAAGATTTTGACCTCTGCACAGCAGCAGGATTTGTAGACCCTCCTCTTTGCTTGTGTCTCTCTGCTCTGCCTCCCTGATATGTGTTTCCTTGGTCAGATTCCTCTCCTTTCTTTGTCTCATAACCTGGCAGAACTGCATCTTCCTGTGTCTTCTTACTCTTTGCATGCTTCTTTGCCTTCAACACCTTCATAACCTCTTGATTAGTAATGAGCCTATAAGCATGACCAAGATTAAGAGTCTCATTAGGCCTAAGCAACTTAACACGAGTAAACTTCACAGTCTTTTGTTCTTGATTCTGCTCTTCTGGTAGAACAGGTAATGGAATAATCAAAGAAACATAGTGACCTGGGTTTGTTTTCATAACCTCACTAGCAGTAACTGGCCAATACAATCTATCTATCTTCCCACAAGGATGTTGAATCACAAGAACAGCAGCATCCACAGCTTGACAATTTCCCATGGCAAAGATAAGAGAAAATGGATGAATGAATGAATGAATGAAGGAAAGAAAAGTCTCTATCTATACAGCTTGTAGTAGCACCCCACAAATATAGGAAAATCAGGTTGGAAGAGGTAGTCTTTGTTTTTGTTCTCTAATAATAATAACAAGGCTTCTCTTCACAACAACTAAATGTCCCGCGTACTCTGCGTTG
TARGET=787,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1486
PRIMER_LEFT_PENALTY=0.073038
PRIMER_RIGHT_PENALTY=0.075553
PRIMER_LEFT_SEQUENCE=GTTGAATCACAAGAACAGCAGC
PRIMER_RIGHT_SEQUENCE=TTTTCCTATATTTGTGGGGTGC
PRIMER_LEFT=719,22
PRIMER_RIGHT=863,22
PRIMER_LEFT_TM=59.927
PRIMER_RIGHT_TM=60.076
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=0.1749
PRIMER_LEFT_1_PENALTY=0.073038
PRIMER_RIGHT_1_PENALTY=0.101896
PRIMER_LEFT_1_SEQUENCE=GTTGAATCACAAGAACAGCAGC
PRIMER_RIGHT_1_SEQUENCE=CGGGACATTTAGTTGTTGTGAA
PRIMER_LEFT_1=719,22
PRIMER_RIGHT_1=943,22
PRIMER_LEFT_1_TM=59.927
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=225
PRIMER_PAIR_PENALTY_2=0.1871
PRIMER_LEFT_2_PENALTY=0.111534
PRIMER_RIGHT_2_PENALTY=0.075553
PRIMER_LEFT_2_SEQUENCE=GAATCACAAGAACAGCAGCATC
PRIMER_RIGHT_2_SEQUENCE=TTTTCCTATATTTGTGGGGTGC
PRIMER_LEFT_2=722,22
PRIMER_RIGHT_2=863,22
PRIMER_LEFT_2_TM=59.888
PRIMER_RIGHT_2_TM=60.076
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.2134
PRIMER_LEFT_3_PENALTY=0.111534
PRIMER_RIGHT_3_PENALTY=0.101896
PRIMER_LEFT_3_SEQUENCE=GAATCACAAGAACAGCAGCATC
PRIMER_RIGHT_3_SEQUENCE=CGGGACATTTAGTTGTTGTGAA
PRIMER_LEFT_3=722,22
PRIMER_RIGHT_3=943,22
PRIMER_LEFT_3_TM=59.888
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.3025
PRIMER_LEFT_4_PENALTY=0.226971
PRIMER_RIGHT_4_PENALTY=0.075553
PRIMER_LEFT_4_SEQUENCE=AGTGACCTGGGTTTGTTTTCAT
PRIMER_RIGHT_4_SEQUENCE=TTTTCCTATATTTGTGGGGTGC
PRIMER_LEFT_4=644,22
PRIMER_RIGHT_4=863,22
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=60.076
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_05931 Ps_contig_mira-and-tgicl-ass_franssen_40621
SEQUENCE=GGTTTAAGTTGTAACTATANTCAAGAACAAAACTACAATTCTAATAGATATTCACATTCATTACTTTTCATTCCATTCTTGTTGTTGCTATACAAACATGTTACTCATTCTAGCATGGTTTTGCAGCTAGAATGCATCATTTGTTGATGGAAGAGTAATCTCGGTTCAAACCTCAGTTGAATTTCCATTAATTCCCATTTACCAGGAACCATCCATGAGGAACATTCAACATTTATAGAGGAAGACAATTAATAGTCATTATTAAACTTTTGGTGACTCCGTGATGCTTTGTAAAGAAGGGCGCCAAGATTTTGACCTCTGCACAGCAGCAGGATTTGTAGACCCTCCTCTTTGCTTGTGTCTCTCTGCTCTGCCTCCCTGATATGTGTTTCCTTGGTCAGATTCCTCTCCTTTCTTTGTCTCATAACCTGGCAGAACTGCATCTTCCTGTGTCTTCTTACTCTTTGCATGCTTCTTTGCCTTCAACACCTTCATAACCTCTTGATTAGTAATGAGCCTATAAGCATGACCAAGATTAAGAGTCTCATTAGGCCTAAGCAACTTAACACGAGTAAACTTCACAGTCTTTTGTTCTTGATTCTGCTCTTCTGGTAGAACAGGTAATGGAATAATCAAAGAAACATAGTGACCTGGGTTTGTTTTCATAACCTCACTAGCAGTAACTGGCCAATACAATCTATCTATCTTCCCACAAGGATGTTGAATCACAAGAACAGCAGCATCCACAGCTTGACAATTTCCCATGGCAAAGATAAGAGAAAATGGATGAATGAATGAATGAATGAAGGAAAGAAAAGTCTCTATCTATACAGCTTGTAGTAGCACCCCACAAATATAGGAAAATCAGGTTGGAAGAGGTAGTCTTTGTTTTTGTTCTCTAATAATAATAACAAGGCTTCTCTTCACAACAACTAAATGTCCCGCGTACTCTGCGTTG
TARGET=900,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1749
PRIMER_LEFT_PENALTY=0.073038
PRIMER_RIGHT_PENALTY=0.101896
PRIMER_LEFT_SEQUENCE=GTTGAATCACAAGAACAGCAGC
PRIMER_RIGHT_SEQUENCE=CGGGACATTTAGTTGTTGTGAA
PRIMER_LEFT=719,22
PRIMER_RIGHT=943,22
PRIMER_LEFT_TM=59.927
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.1774
PRIMER_LEFT_1_PENALTY=0.075553
PRIMER_RIGHT_1_PENALTY=0.101896
PRIMER_LEFT_1_SEQUENCE=GCACCCCACAAATATAGGAAAA
PRIMER_RIGHT_1_SEQUENCE=CGGGACATTTAGTTGTTGTGAA
PRIMER_LEFT_1=842,22
PRIMER_RIGHT_1=943,22
PRIMER_LEFT_1_TM=60.076
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=0.2134
PRIMER_LEFT_2_PENALTY=0.111534
PRIMER_RIGHT_2_PENALTY=0.101896
PRIMER_LEFT_2_SEQUENCE=GAATCACAAGAACAGCAGCATC
PRIMER_RIGHT_2_SEQUENCE=CGGGACATTTAGTTGTTGTGAA
PRIMER_LEFT_2=722,22
PRIMER_RIGHT_2=943,22
PRIMER_LEFT_2_TM=59.888
PRIMER_RIGHT_2_TM=59.898
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.2735
PRIMER_LEFT_3_PENALTY=0.171564
PRIMER_RIGHT_3_PENALTY=0.101896
PRIMER_LEFT_3_SEQUENCE=TACAGCTTGTAGTAGCACCCCA
PRIMER_RIGHT_3_SEQUENCE=CGGGACATTTAGTTGTTGTGAA
PRIMER_LEFT_3=828,22
PRIMER_RIGHT_3=943,22
PRIMER_LEFT_3_TM=59.828
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=11.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=116
PRIMER_PAIR_PENALTY_4=0.3289
PRIMER_LEFT_4_PENALTY=0.226971
PRIMER_RIGHT_4_PENALTY=0.101896
PRIMER_LEFT_4_SEQUENCE=AGTGACCTGGGTTTGTTTTCAT
PRIMER_RIGHT_4_SEQUENCE=CGGGACATTTAGTTGTTGTGAA
PRIMER_LEFT_4=644,22
PRIMER_RIGHT_4=943,22
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=300
=
PRIMER_SEQUENCE_ID=SSR_05932 Ps_contig_mira-and-tgicl-ass_franssen_40626
SEQUENCE=GGTCCTACGAAGTATGGATAGTCAATTACTACTACTACTACCGTGTCTACTATATATTTGTTTGATAATGGCGGCCTGTATGTAGTTTCTGTAATATTTATTATGTAATATCCCAGAATATGGAATTACCCTCTACAGTATGCTTCAGATTTGTGAATGACAAGGTTCTAGCTTACTTAAGTT
TARGET=27,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=6.6707
PRIMER_LEFT_PENALTY=3.181806
PRIMER_RIGHT_PENALTY=3.488905
PRIMER_LEFT_SEQUENCE=GGTCCTACGAAGTATGGATAGTCAA
PRIMER_RIGHT_SEQUENCE=AAGCATACTGTAGAGGGTAATTCCA
PRIMER_LEFT=0,25
PRIMER_RIGHT=144,25
PRIMER_LEFT_TM=59.818
PRIMER_RIGHT_TM=59.511
PRIMER_LEFT_GC_PERCENT=44.000
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=6.7061
PRIMER_LEFT_1_PENALTY=3.181806
PRIMER_RIGHT_1_PENALTY=3.524306
PRIMER_LEFT_1_SEQUENCE=GGTCCTACGAAGTATGGATAGTCAA
PRIMER_RIGHT_1_SEQUENCE=CAAATCTGAAGCATACTGTAGAGGG
PRIMER_LEFT_1=0,25
PRIMER_RIGHT_1=152,25
PRIMER_LEFT_1_TM=59.818
PRIMER_RIGHT_1_TM=60.524
PRIMER_LEFT_1_GC_PERCENT=44.000
PRIMER_RIGHT_1_GC_PERCENT=44.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=153
PRIMER_PAIR_PENALTY_2=6.7925
PRIMER_LEFT_2_PENALTY=3.390574
PRIMER_RIGHT_2_PENALTY=3.401915
PRIMER_LEFT_2_SEQUENCE=GGTCCTACGAAGTATGGATAGTCA
PRIMER_RIGHT_2_SEQUENCE=GCTAGAACCTTGTCATTCACAAATC
PRIMER_LEFT_2=0,24
PRIMER_RIGHT_2=171,25
PRIMER_LEFT_2_TM=58.609
PRIMER_RIGHT_2_TM=60.402
PRIMER_LEFT_2_GC_PERCENT=45.833
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=6.8151
PRIMER_LEFT_3_PENALTY=3.181806
PRIMER_RIGHT_3_PENALTY=3.633326
PRIMER_LEFT_3_SEQUENCE=GGTCCTACGAAGTATGGATAGTCAA
PRIMER_RIGHT_3_SEQUENCE=GCATACTGTAGAGGGTAATTCCA
PRIMER_LEFT_3=0,25
PRIMER_RIGHT_3=142,23
PRIMER_LEFT_3_TM=59.818
PRIMER_RIGHT_3_TM=57.367
PRIMER_LEFT_3_GC_PERCENT=44.000
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=143
PRIMER_PAIR_PENALTY_4=6.8645
PRIMER_LEFT_4_PENALTY=3.181806
PRIMER_RIGHT_4_PENALTY=3.682701
PRIMER_LEFT_4_SEQUENCE=GGTCCTACGAAGTATGGATAGTCAA
PRIMER_RIGHT_4_SEQUENCE=AGCATACTGTAGAGGGTAATTCCA
PRIMER_LEFT_4=0,25
PRIMER_RIGHT_4=143,24
PRIMER_LEFT_4_TM=59.818
PRIMER_RIGHT_4_TM=58.317
PRIMER_LEFT_4_GC_PERCENT=44.000
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=144
=
PRIMER_SEQUENCE_ID=SSR_05933 Ps_contig_mira-and-tgicl-ass_franssen_40634
SEQUENCE=GGGGTCCTTAACAAGAGATTGCATATGTCCCATGGTCCTCACTCTCTCTCTACAAAAAGGACAAAAGGAAACGAAATAGAARAAAGAGGAAGGAATAACTTTCTCTGAAAACCGCTGAAAGAAAATCCTCATCTTCTCAGAAAAGCCACAAAGGCAGTCCCTCGTTTTGCTTTCCATCATCCACCATCACCTTCGTCTTTTCAAAAACTCTTTCCTCTTCTGCACAACAAAAATACCACGCTCTCACTTCCACCACCAACAAACAGCGTACATACTTCATCTTCTCCGCAGAACCCACACGTTTTCTCCATCGTCCACTCTGTCCAGCTCCTTCACAAACGGCGACGACGCCGTCACCAACCTCCGTCAGCTTCCAAACACAACACCGCCACTCACTTCCTTCCCCCGATTTCTGATGAACAACCTCCCTCCTCCGTTGATACTCCCTCCATCCGGTAACAACCATCTCCATCGACGCGATTCACCGTGTAAACTTCTCCACCATACCAACAACACCTCTGTTGCAGGAACATAAGGCCTACAATCGAAGTTGTTCAGCTACGATGACAACACTGTTATAACAAGGCGTCGATTCGAATCGGTTACTGGTTGACGATTTGATTTGAACGGAACGTTTTGACATTATGTGCTACTAGTGTTGTTTTGACACTACATCATCCGTGTTACTGCTACGACTCAGTTATATGTGAATTACACTTCGGCTAAAATATTGTTTTATATCTTTGATTTACATTATCGAGATGTTTAGTTTTGAGTTTATGTAATCTATTTTCTACTTTATGGAATTATA
TARGET=42,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05934 Ps_contig_mira-and-tgicl-ass_franssen_40637
SEQUENCE=TAGTAAAGAGCAACTTTGTAACTCAACAAATATAATAAACCAAGTAGTCTAGTAGATTAGTAGCAACTACCAAAGGGTTTGAAAACCATAAATAACTACCAACTTTACATCAGTCAAAGTCCTAGTTCCAATCTTTTTACATGCAAAAAAAAACATTAACACATAAACTAATGACTATTAGTATTATCTTGATCCCAATCATTTCGTTATCAGCCACTACCAGACATAATTGACATTGCCCGTCTGAAATCCAAGGCTGTTCGGGGGAAAGGCTTTCCACCTGGTAGCCACAAGTCACCTTCATGAGGCAGCAGCACTTGTGGATGGCCCTGGTTATTTTTACCACCTGCAACAGCTTCCTCAGATAGAACTTGCTGTTTCTTGACAAATTCAAAAAACTCTGCAACTTGGCGTGCATATTCCCTCTTGGCTTCAATATCCTTGGTGAAATCAATATTGGGTTCACAGTCCAGAATCAATGCAGGAACCTTCTGAATGCTGGAATGCATGTGGTCACCCTCTAGATAAAATACACGGTCTCTTATATCAGGGTGTAAGGAGTTGTCGACATGACGGGGCAGCTTATTGATAGATAACACTCCATGATTACCACTTTGGGAGGGGAATAACCAGCTCTCATGCTTTTCGTGGAGGTCGCGCAGATACTCTAGGCTGACTCCGCCTTCTTCTTCTCTCTTCCTTGATTTCATTCTTTGATGGCAAGTATCAGGACTTGCTCTGAGATAGATAAAACCATCAGGAATGAGGCCGGGCAAGGTAGACACAACAGGATCAAACCAAGAATCATAAATACTGATCTCCATCTCATTCATCCACTTGGCTTCATGAACTGCTCGTACAAAAACCATCCTGTCACTGAAAACACTCCTCTCCATCAATCGAAGAGGCTTGATTCCAGCAGAAGACTCTCTCTCTTGCATCACCCTAGTAACAAAAACGTAGTTCTGAAAGGTGTATGCATACCTCTCTGGCTCAGCATAAAAAGCATCTAGGATATTGAAGTGGTCGGGTCCAATATCTTGCCACTTACCAATGGGTTCAGGAACCACCTCGACGAGATCCCTCAGTTCAATAGTTTCATTGGCTATTCTCTGAAGAAAAGTAGTCTTACCAACACTGATATTACCCTCGACACAGAACGTAAGTCGCTT
TARGET=927,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2122
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.203971
PRIMER_LEFT_SEQUENCE=CACAACAGGATCAAACCAAGAA
PRIMER_RIGHT_SEQUENCE=TAAGTGGCAAGATATTGGACCC
PRIMER_LEFT=782,22
PRIMER_RIGHT=1049,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.204
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=0.2122
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.203971
PRIMER_LEFT_1_SEQUENCE=CAAAAACCATCCTGTCACTGAA
PRIMER_RIGHT_1_SEQUENCE=TAAGTGGCAAGATATTGGACCC
PRIMER_LEFT_1=859,22
PRIMER_RIGHT_1=1049,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.204
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.2357
PRIMER_LEFT_2_PENALTY=0.031769
PRIMER_RIGHT_2_PENALTY=0.203971
PRIMER_LEFT_2_SEQUENCE=ATCTCATTCATCCACTTGGCTT
PRIMER_RIGHT_2_SEQUENCE=TAAGTGGCAAGATATTGGACCC
PRIMER_LEFT_2=822,22
PRIMER_RIGHT_2=1049,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=60.204
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=228
PRIMER_PAIR_PENALTY_3=0.2830
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.274705
PRIMER_LEFT_3_SEQUENCE=CACAACAGGATCAAACCAAGAA
PRIMER_RIGHT_3_SEQUENCE=ACCCGACCACTTCAATATCCTA
PRIMER_LEFT_3=782,22
PRIMER_RIGHT_3=1031,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.725
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.2830
PRIMER_LEFT_4_PENALTY=0.008257
PRIMER_RIGHT_4_PENALTY=0.274705
PRIMER_LEFT_4_SEQUENCE=CAAAAACCATCCTGTCACTGAA
PRIMER_RIGHT_4_SEQUENCE=ACCCGACCACTTCAATATCCTA
PRIMER_LEFT_4=859,22
PRIMER_RIGHT_4=1031,22
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=59.725
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_05935 Ps_contig_mira-and-tgicl-ass_franssen_40638
SEQUENCE=TGGGTTGAGCTTTCTGGTAAAATGCAAGTCTTGGCAAGGTTACTTGCTCAATTACGTCAGAGAACAAATGATCGCATTGTTCTTGTCTCAAACTATACTCAGACTCTTGATCTTTTTGCTCAATTGTGTCGGGAACAAAGGTACCCTCACTTAAGGCTTGATGGCGCTACATCAATCAGTAAAAAGGCAAAAGTTGGTCAACTGCTTGAATGACCCATCTAAGGATGAATTTGTTTTTCTTTTAAGCAGCAAGGCTGGTGGTTGTGGACTCAATTTAATTGGGGCAAATCGACTCGTCTTGTTTGATCCTGATTGGAATCCAGCAAATGATAAACAAGCTGCAGCAAGAGTGTGGAGGGATGGACAGAAGAAAAGAGTTTATATTTATAGATTTTTGAGTGCCGGAACAATAGAAGAAAAGGTCTACCAGCGTCAAATGTCAAAAGAAGGGTTGCAAAAGGTTATTCAGCGAGAGCAAAATGACAGCGTCGCGGCACAGGGTAACTTTCTTTCGACAGAGGATCTTCGCAATTTGTTTACTTTTGATGAGAACGTCAAGTCAGATATTCATGAAAATATGCGATGCAGNCGGTGCCAAACATATGATGGCCCTCAAGACACTGATGTCTTATCGACAATGATTAATAGCGAACATGATGATGATGAAACCTNTGATATAGG
TARGET=654,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05936 Ps_contig_mira-and-tgicl-ass_franssen_40639
SEQUENCE=AGAGAATATTTTCTACAGAGATCGAAATCGAAGTTTGTNGRAAGGAAATTTTGACGAGTCTGGATCTGTTGGTGGAGGGAGCAAAGCGCCGCCGATGGGTGATATGAAGAGATTTGCGAGTGGGCGTGAAGCTTTTTCGAGTTTCAATTGGAAAGCTGTGATTGAGCCAGAGGAAATTGATGAGAGGGATTGCTTTACGGATGCAGAAGAGGATGAGATCTTGATTCCATTTTCTGCACCAATTTTGGTTGGTGGAGGCGGTGAAGGTGATGATTCTAGACCTAGTTTGAAGTTTAAGCCAAGGAATGAAATAAATCTTTGGAAGAGAAGAACAATGGCACCGCCAAGGCCTCTTCAGTTGAATCCTGTGCTTAAGGCTAAGTAAATTGTTAATTCTTTCATAATCAATAACTTGTTCAAATTGTAGTATTTCTTCATTCTTTTATATATATACTATTATTTTTTGTANANNNCTTTTGATGCTCATATAATATATTTCTTCTTTACTTAAAGTTAAAACTTT
TARGET=444,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05937 Ps_contig_mira-and-tgicl-ass_franssen_40649
SEQUENCE=GGTAACGAAGTCGGGGAGACAAAAATGGTAGCCATGGAAGAATTCAAGCACTTTGTGATTGTTAAGTTCAAAGAAGGTGTAGTCGTGGAAGACCTCACAAAAGGAATGGAGAAGCTGGTCTCAGAGATTGATCTTGTCAAATCCTTTGAATGGGGACAGGATATTGAAAGCCTAGATGTGTTGAGACAAGGCTTCACTCATGCATTCTTGATGACATTCAACAAGAAAGAAGATTTTGCTGCATTTCAGAGCCATCCAAATCATGTTGAGTTTTCTGAAAAGTTTTCTGCAGCTATTGAGAATATTGTGCTACTCGATTTTCCATCGACACTTGTGAAGCCGGTGAAGGCGGCAGAAGAATAATCTTGAAGAATGGAGATTTTTCAGAAATAATGATTTCATCTTTTTGAAGATGAAGAATGTTAAGTGTGTGTGTTTGTGAATGTATTTTAGTTGTATGTTGTTATTGTTACTATATGTGCGATTTGAATTGACAATGGTGTTTCTAAAGTTTTTTTGGTCCTAANTTTTTTTTTGTTAGAAACTAGCTTT
TARGET=427,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05938 Ps_contig_mira-and-tgicl-ass_franssen_40652
SEQUENCE=ACCCTTGATTTTGAATCCACGATAGATAAACAAGATAAGGTGTTTTTGTTTTTGCGTGTGTGTGTATGCGAAGGAAGAAAGAGTTTTTCGAGCAATGGGAAGAGCGTTCGTGTACGTGATTCTCGGTGGTGGTGTATCTGCTGGTTATGCTGCTCTTGAATTCGTTAGACGAGGTGTTTCTCATGGTGAACTCTGTATCATTTCTAATGAATCAGTTGCTCCGTATGAAAGACCTGCATTAAGCAAAGGATATTTGCTTCCAGAAGCTGCTGCCCGGCTTCCATCCTTTCATACATGTGTAGGAGCAAATGAGGAAAGGCTAACTCCAAAATGGTATAAAGAACATGGAGTTGAACTAGTTCTTGGAACTGGAGTTAAGTCTGCTGATGTGAAACGTAAAACACTGTTAACCACAACTGGGGAGACCATAAGTTACAAATTYCTTATTATTGCTACTGGCGCCCGGGCTTTGAAACTGGAGGAATTTGGGGTTAATGGA
TARGET=56,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05939 Ps_contig_mira-and-tgicl-ass_franssen_40659
SEQUENCE=TTGGAAATGTTAAAAGTTAGAGCTTCTCTTGAGGAAAAGTTGCAACAAAAGGGTTTGTATAATCAAACTGCTCCAAAGCCAAGTAATTCTCTAAAGCGTTCAAGACCGGCTAATGGCCAGCTTGCTACATATGATGATTTTGATGATGATGCTGTCAATGTTAATGGGACAGCAGGACTATCTAATGGTCGTGTCTCCGGTAAAGTTTCAGAGTTTGTTAATGCTAATCTGAAGAAATTCAAGGTGGCTTCTGGTGATGATGATTTGCCCAAGAGAGATGGTATTCTTAATCGCCGGCGGCAACATGAAGTCCGTGTGTTGGCTGGTGCTAGCGTTAAAACTGAGGATGACAAAGGTGATGATGATGATCAAATGACTGATCTTGGAGCTCATAAAGTTGATGATAAAAAGGTCACTGAAAGCGGAGGTGACCCAGAAAATGAGGTTTATAAACAAGCAGAAACTTTGCTAGCTGCAAAACGTGCTGCAAAAAGTGCTGCAAAAGCAGCGACCCATTCAAGGAAATCAGCTGTCCCCTCTTTGCCAGAGGAGACCGTGGACGGAAAGCGCTATATCACTTCTCAGATGGCAAAGAACAGAGGACTAACTCGAAGTCGCAACAAGAATAAAAAGAATCCTAGAAAGAATTACAAGCTCAAGCATCAAAAAGCTCTTAAGAATCGGGGAGGACAGGTTCAGAATTTCAGAACGCAGACTGCACCTTATGGCGGGGAAGCCTCTGGAATTAATCCAACTATCAGTCGAAGTGTCAGATTCAAGAGCTGATTTTGTCAATATACACATTACAATATCATTCAATTTTTCCACCAATTTTGAATCATATTAGCTGCGTCTGGAGTATAACTTGTATAATTATTTATTAGAAACTGAACATCTTTGGCATATTAAATTTTGTCAAGCTGTCAATTTTGGTCCAAGTATTTCAGTGTTATGTTTATTTTCAAATTATTGAATGACAACAACGGAACTCAAGGCATGAGATTCAACCCCTGAGAAGGTGAATTATTACATTACTTATAC
TARGET=357,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3149
PRIMER_LEFT_PENALTY=0.307953
PRIMER_RIGHT_PENALTY=0.006945
PRIMER_LEFT_SEQUENCE=GGTGCTAGCGTTAAAACTGAGG
PRIMER_RIGHT_SEQUENCE=TTCTTTGCCATCTGAGAAGTGA
PRIMER_LEFT=324,22
PRIMER_RIGHT=595,22
PRIMER_LEFT_TM=60.308
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.3149
PRIMER_LEFT_1_PENALTY=0.307953
PRIMER_RIGHT_1_PENALTY=0.006945
PRIMER_LEFT_1_SEQUENCE=GGTGCTAGCGTTAAAACTGAGG
PRIMER_RIGHT_1_SEQUENCE=TCTGTTCTTTGCCATCTGAGAA
PRIMER_LEFT_1=324,22
PRIMER_RIGHT_1=599,22
PRIMER_LEFT_1_TM=60.308
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.3584
PRIMER_LEFT_2_PENALTY=0.307953
PRIMER_RIGHT_2_PENALTY=0.050443
PRIMER_LEFT_2_SEQUENCE=GGTGCTAGCGTTAAAACTGAGG
PRIMER_RIGHT_2_SEQUENCE=GTTGCGACTTCGAGTTAGTCCT
PRIMER_LEFT_2=324,22
PRIMER_RIGHT_2=620,22
PRIMER_LEFT_2_TM=60.308
PRIMER_RIGHT_2_TM=59.950
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=297
PRIMER_PAIR_PENALTY_3=0.3673
PRIMER_LEFT_3_PENALTY=0.034102
PRIMER_RIGHT_3_PENALTY=0.333229
PRIMER_LEFT_3_SEQUENCE=TTTGCCCAAGAGAGATGGTATT
PRIMER_RIGHT_3_SEQUENCE=CCGCTTTCAGTGACCTTTTTAT
PRIMER_LEFT_3=263,22
PRIMER_RIGHT_3=424,22
PRIMER_LEFT_3_TM=59.966
PRIMER_RIGHT_3_TM=59.667
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=0.3731
PRIMER_LEFT_4_PENALTY=0.039879
PRIMER_RIGHT_4_PENALTY=0.333229
PRIMER_LEFT_4_SEQUENCE=GTCGTGTCTCCGGTAAAGTTTC
PRIMER_RIGHT_4_SEQUENCE=CCGCTTTCAGTGACCTTTTTAT
PRIMER_LEFT_4=187,22
PRIMER_RIGHT_4=424,22
PRIMER_LEFT_4_TM=60.040
PRIMER_RIGHT_4_TM=59.667
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=238
=
PRIMER_SEQUENCE_ID=SSR_05940 Ps_contig_mira-and-tgicl-ass_franssen_40664
SEQUENCE=TAACTTGTTACGGAGTTTTTGGTTTTTTGGGTTGGGTTGGGTGGAAATAGGTTAGGAACNGTTTAGGTAGTAGACAATGATGAACTGGTGGTGGAGGAGAAGGANATTGTACGGCTTATGGTGCGGTGGCGGAGATTTGTTAGTAGTAAGGTGGTGGAGGTGAGGGAGAGGGTGGTGGTGGAGATTTATAGTAGTAAGGAGGTGGAGGTGATGAAGAAGGTGGTGGAGGAGACTTGTACTCATAAGGAGGTGGGGGTGAGGCAGATGGTGGTGGAGGTGACTTGTACTCATATGGTGGAGCCTTATGTTCAATGGGTGGTGGAGGGGGTGACTTGTACTCATAAGGAGGTTCAATGGGTGGTGGAGGGGGTGACTTGTACTCATAAGGAGGTGGTGGTGAAGGAGTTGATGGTGGAGGTGACTTGTACTCATAAGTTGGTGGTGGAGGAGAACTGTATGGGTAACCATCAGCAGCAACAGAAACCACAGTAGTAGAAATCAGAACAATGGCCAATGCCATGGCCATTTCCGGCCGAAACCGACCCCTTATGGGGTCACTGCCCCTAGCAGTCATGGGCAATAGGCAAAAGCTGGCCAATGCTTCTGAGAGTATCCTCACCACTATTTAGAAAAGCTGAAAAGAGAGAGATAGAGAAAGAGGAGGGGAAGGTTTTGTGTTAGTGATGACTCCCCGAC
TARGET=27,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05941 Ps_contig_mira-and-tgicl-ass_franssen_40665
SEQUENCE=ACCACAAATTTCCCCCCTTCATATCTCCACACACACACTACCTCTTCAATCAGCTATATATATATTTATGGTTCATTAGTCTATNATTCAAGTATTCAAACAACAACTTGATACACCTTAATTTCCCATATTTTCATAACTTGTTCACACAGCAAAGTTTGAAGTATAAAGAGAATTTATTAATGGCTGCTACTGCTACTGCTGGTTCATTTTCAATGAAGGGTTCGAAGGTTCGATCGCTGGGACGATACAGTTCGAAGCAAGTTCGTCAGCAGAGAGCAAGGTTATACATAATATGGAGATGTACTGTTTTACTATTGTGTTGGAATGATTAAAAGTCAGTTCAAGAAGATGAAGATTTGAGAGTTTGTATTGTTTCTAATTGCAATTGTAGGTGCTGYCATGGGAGCTTTGGGGTGTGAAATTATTAACACTCAAAGGTGATGCAGTAGTTTTTTTTACTACTGGGTAGGGTAGGTGAAGGAATATAACTAAGGAGCTAGATGAAGCAGGAGTTTCAGCTTAAACTGTAAATTACAATTAGTATTCAGCCATGTATAGATATAATGATAATTATAATGATTAATTCAAGTTGAAATTTTACTTAACT
TARGET=28,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05942 Ps_contig_mira-and-tgicl-ass_franssen_40665
SEQUENCE=ACCACAAATTTCCCCCCTTCATATCTCCACACACACACTACCTCTTCAATCAGCTATATATATATTTATGGTTCATTAGTCTATNATTCAAGTATTCAAACAACAACTTGATACACCTTAATTTCCCATATTTTCATAACTTGTTCACACAGCAAAGTTTGAAGTATAAAGAGAATTTATTAATGGCTGCTACTGCTACTGCTGGTTCATTTTCAATGAAGGGTTCGAAGGTTCGATCGCTGGGACGATACAGTTCGAAGCAAGTTCGTCAGCAGAGAGCAAGGTTATACATAATATGGAGATGTACTGTTTTACTATTGTGTTGGAATGATTAAAAGTCAGTTCAAGAAGATGAAGATTTGAGAGTTTGTATTGTTTCTAATTGCAATTGTAGGTGCTGYCATGGGAGCTTTGGGGTGTGAAATTATTAACACTCAAAGGTGATGCAGTAGTTTTTTTTACTACTGGGTAGGGTAGGTGAAGGAATATAACTAAGGAGCTAGATGAAGCAGGAGTTTCAGCTTAAACTGTAAATTACAATTAGTATTCAGCCATGTATAGATATAATGATAATTATAATGATTAATTCAAGTTGAAATTTTACTTAACT
TARGET=55,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05943 Ps_contig_mira-and-tgicl-ass_franssen_40667
SEQUENCE=TTCCTTGTTCCATTCTCCTTCACTTTCACCTTCTCCTTATCCGCTGAACCGGTTTCACCGATAAACCACTGAACCGGTTTCTCACTTCCTTTGGAACTAAACCCGAGTCTTTTCAAATTCCCGTCCCATGAATGTTTCAAAACCGAAACGCTTCTATGAATCACGCGTTTGTTCCTGTTTACCAAGTAAAGCGCTACAGCCACGAATATTAGAATCAGCAACACGTTCTCGGAAGTGGGACCCACTGACCCAATATAGAAAAAATAGAAAACGTAGAAGAAGAGTGAACACCCGCTGAAAAAACCTAGAATCGGAGCAGCAACGAACCGGATCGAACCAGAACCTGAACCGGAATCGAACCCTCGCTTCTTCTTATTCTTTTTATTTCCGTTTCTTCTTCTTCTTATCGAGTTTCTCTACATCGTAAAACTCGTCTTCATTTACTGAACCGAGGCTATGAATGGAGGATCTGTTGGTCGGAGAAGAACACCGAAGCAGAGAGGATTTGGTTCGGGCGAGCTTGCTTTGGCTATTCGTATTCTGAGTTTCCCCCGAC
TARGET=392,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1347
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=0.060196
PRIMER_LEFT_SEQUENCE=GGACCCACTGACCCAATATAGA
PRIMER_RIGHT_SEQUENCE=CAGATCCTCCATTCATAGCCTC
PRIMER_LEFT=237,22
PRIMER_RIGHT=471,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=60.060
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=0.2627
PRIMER_LEFT_1_PENALTY=0.074466
PRIMER_RIGHT_1_PENALTY=0.188254
PRIMER_LEFT_1_SEQUENCE=GGACCCACTGACCCAATATAGA
PRIMER_RIGHT_1_SEQUENCE=CTCTCTGCTTCGGTGTTCTTCT
PRIMER_LEFT_1=237,22
PRIMER_RIGHT_1=501,22
PRIMER_LEFT_1_TM=60.074
PRIMER_RIGHT_1_TM=60.188
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.2724
PRIMER_LEFT_2_PENALTY=0.074466
PRIMER_RIGHT_2_PENALTY=0.197963
PRIMER_LEFT_2_SEQUENCE=GGACCCACTGACCCAATATAGA
PRIMER_RIGHT_2_SEQUENCE=GACCAACAGATCCTCCATTCAT
PRIMER_LEFT_2=237,22
PRIMER_RIGHT_2=477,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=60.198
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=0.5007
PRIMER_LEFT_3_PENALTY=0.440473
PRIMER_RIGHT_3_PENALTY=0.060196
PRIMER_LEFT_3_SEQUENCE=TAGAATCAGCAACACGTTCTCG
PRIMER_RIGHT_3_SEQUENCE=CAGATCCTCCATTCATAGCCTC
PRIMER_LEFT_3=209,22
PRIMER_RIGHT_3=471,22
PRIMER_LEFT_3_TM=60.440
PRIMER_RIGHT_3_TM=60.060
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=0.6276
PRIMER_LEFT_4_PENALTY=0.074466
PRIMER_RIGHT_4_PENALTY=0.553159
PRIMER_LEFT_4_SEQUENCE=GGACCCACTGACCCAATATAGA
PRIMER_RIGHT_4_SEQUENCE=AACAGATCCTCCATTCATAGCC
PRIMER_LEFT_4=237,22
PRIMER_RIGHT_4=473,22
PRIMER_LEFT_4_TM=60.074
PRIMER_RIGHT_4_TM=59.447
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=237
=
PRIMER_SEQUENCE_ID=SSR_05944 Ps_contig_mira-and-tgicl-ass_franssen_40679
SEQUENCE=ACGGGGGAACCTCATCAAACTAAAAACAACAACTCTCTCTCTCCTTCTTCTGTCTTCTACAAAACCACATTCACACTCCTACATAAAGTTCTGTCTTTTTTCATACAATCACCAACCCATTTCCTTCAAATCCTCAAAACCAACCTCCATTCACCCTTTCATGCAAAGATTCCATCTGGGTTTCTCTCAAAATCCAACCTTGATGATGGATTCTTCTTCATCTCCTCTCTCTCTTCAAAACCCATCCTCCTCACCAAAACCCACCAAAAAAACCTTACTTTCATCTCTAATTGGCACCAACACTCCTTCATCTCTTCGAACCCCTTCCTTTGAAGAAGACAACTACTTTGTCACCCTTCTCAAATCCTCAGAGAAAAAAGCACTTCAAGACCTCAAAAAACTCCTCCTTGAAGCCACCACCACCTCATCCAATGAAGTTTCCATGTGGGGTGTTCCTCTCCTTGGCGGCGACGACAGAGCTGATGTAATCCTCTTGAAGTTCCTCAGAGCAAGAGACTTCAGAGTCAATGAATCCCTCAACATGATTCTAAAGTGCTTATCTTGGAGAAAAGAGTTCTCTGCTGACACCATAGTGGAAGAGGATTTAAGTCAGTTCAAGGAACTAGAAGGTGTTATAGCTTATATGCAAGGCTATGACAAAGAGGGTCACCCTGTTTGTTACAATGCTTATGGTTTGTTTAGAGACAAAGACATGTATGAGAGATTCTTTGGTGATGAAGAGAAGCTCAAGAAGTTTCTTAGATGGAGAGTTCAAGTTCTTGAAAGAGGTATTAGGCTTTTACATTTTAAACCAGGCGGTGTTAATTCTCTTATTCAAGTAACCGATCTAAAAGATATGCCTAAAAGAGAGTTGAGGGTTGCTTCTAATCATATTATGTCACTTTTTCAAGATAATTACCCTGAAATGGTTGCTAGAAAGATCTTCATCAATGTGCCATGGTATTTCAGCATGTTGTATTCAATGTTTAGTCCTTTTTTGACTCAAAGGACTAAAAGCAAGTTTGTGATATCAAAAGAAGGAAATGCTGCTGAGACACTCTACAAATTCATTAGACCTGAGGATATTCCTGTTCAGTATGGAGGGCTAAGTTTTCCCAGTGATTTGCAAAATGGTCCCCCAAAACCTGCTTCTGAATTCACTGTCAAAGGAGGAGAGAAGGTGAATATACAAATAGAAGGAATTGAGGGAGGTGCAACAATCACTTGGGACATTGTTGTTGGAGGCTGGGACTTAGAATACAGTGCTGAGTTTGTTCCAAATGCTGAAGGAAGCTACACTATAGCAGTTGAGAAGCCGCGAAAGATTGGATCATCCGAAGAAGCGATCCACAACTCATTCACGTCGAAAGAATCCGGCAAAATGATACTCTCGGTCGACAACACCGCCTCAAGGAAGAAAAAAGTCGCCGCGTATCGTTATGTCGTCCGAAAATGCACCGCAACTTGAACGGATGCTATGGCCTCAAGTAGCTTTTAAATTTTNANTTTTTGCTAATGATTGTTTTTTTGTTTAGTACCTTTTGTTTTTATTGTTTTTTGTAACTTTGTTTTTTATGGCTA
TARGET=33,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7094
PRIMER_LEFT_PENALTY=0.649547
PRIMER_RIGHT_PENALTY=0.059875
PRIMER_LEFT_SEQUENCE=GGGGAACCTCATCAAACTAAAA
PRIMER_RIGHT_SEQUENCE=TTGAGAGAAACCCAGATGGAAT
PRIMER_LEFT=3,22
PRIMER_RIGHT=189,22
PRIMER_LEFT_TM=59.350
PRIMER_RIGHT_TM=59.940
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=187
PRIMER_PAIR_PENALTY_1=0.8295
PRIMER_LEFT_1_PENALTY=0.649547
PRIMER_RIGHT_1_PENALTY=0.179943
PRIMER_LEFT_1_SEQUENCE=GGGGAACCTCATCAAACTAAAA
PRIMER_RIGHT_1_SEQUENCE=GGAAATGGGTTGGTGATTGTAT
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=123,22
PRIMER_LEFT_1_TM=59.350
PRIMER_RIGHT_1_TM=59.820
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=121
PRIMER_PAIR_PENALTY_2=0.9590
PRIMER_LEFT_2_PENALTY=0.649547
PRIMER_RIGHT_2_PENALTY=0.309418
PRIMER_LEFT_2_SEQUENCE=GGGGAACCTCATCAAACTAAAA
PRIMER_RIGHT_2_SEQUENCE=GGATGGGTTTTGAAGAGAGAGA
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=59.350
PRIMER_RIGHT_2_TM=59.691
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.9590
PRIMER_LEFT_3_PENALTY=0.649547
PRIMER_RIGHT_3_PENALTY=0.309418
PRIMER_LEFT_3_SEQUENCE=GGGGAACCTCATCAAACTAAAA
PRIMER_RIGHT_3_SEQUENCE=GAGGATGGGTTTTGAAGAGAGA
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=248,22
PRIMER_LEFT_3_TM=59.350
PRIMER_RIGHT_3_TM=59.691
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=1.0028
PRIMER_LEFT_4_PENALTY=0.649547
PRIMER_RIGHT_4_PENALTY=0.353222
PRIMER_LEFT_4_SEQUENCE=GGGGAACCTCATCAAACTAAAA
PRIMER_RIGHT_4_SEQUENCE=TGGGTTTTGAAGAGAGAGAGGA
PRIMER_LEFT_4=3,22
PRIMER_RIGHT_4=243,22
PRIMER_LEFT_4_TM=59.350
PRIMER_RIGHT_4_TM=60.353
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_05945 Ps_contig_mira-and-tgicl-ass_franssen_40679
SEQUENCE=ACGGGGGAACCTCATCAAACTAAAAACAACAACTCTCTCTCTCCTTCTTCTGTCTTCTACAAAACCACATTCACACTCCTACATAAAGTTCTGTCTTTTTTCATACAATCACCAACCCATTTCCTTCAAATCCTCAAAACCAACCTCCATTCACCCTTTCATGCAAAGATTCCATCTGGGTTTCTCTCAAAATCCAACCTTGATGATGGATTCTTCTTCATCTCCTCTCTCTCTTCAAAACCCATCCTCCTCACCAAAACCCACCAAAAAAACCTTACTTTCATCTCTAATTGGCACCAACACTCCTTCATCTCTTCGAACCCCTTCCTTTGAAGAAGACAACTACTTTGTCACCCTTCTCAAATCCTCAGAGAAAAAAGCACTTCAAGACCTCAAAAAACTCCTCCTTGAAGCCACCACCACCTCATCCAATGAAGTTTCCATGTGGGGTGTTCCTCTCCTTGGCGGCGACGACAGAGCTGATGTAATCCTCTTGAAGTTCCTCAGAGCAAGAGACTTCAGAGTCAATGAATCCCTCAACATGATTCTAAAGTGCTTATCTTGGAGAAAAGAGTTCTCTGCTGACACCATAGTGGAAGAGGATTTAAGTCAGTTCAAGGAACTAGAAGGTGTTATAGCTTATATGCAAGGCTATGACAAAGAGGGTCACCCTGTTTGTTACAATGCTTATGGTTTGTTTAGAGACAAAGACATGTATGAGAGATTCTTTGGTGATGAAGAGAAGCTCAAGAAGTTTCTTAGATGGAGAGTTCAAGTTCTTGAAAGAGGTATTAGGCTTTTACATTTTAAACCAGGCGGTGTTAATTCTCTTATTCAAGTAACCGATCTAAAAGATATGCCTAAAAGAGAGTTGAGGGTTGCTTCTAATCATATTATGTCACTTTTTCAAGATAATTACCCTGAAATGGTTGCTAGAAAGATCTTCATCAATGTGCCATGGTATTTCAGCATGTTGTATTCAATGTTTAGTCCTTTTTTGACTCAAAGGACTAAAAGCAAGTTTGTGATATCAAAAGAAGGAAATGCTGCTGAGACACTCTACAAATTCATTAGACCTGAGGATATTCCTGTTCAGTATGGAGGGCTAAGTTTTCCCAGTGATTTGCAAAATGGTCCCCCAAAACCTGCTTCTGAATTCACTGTCAAAGGAGGAGAGAAGGTGAATATACAAATAGAAGGAATTGAGGGAGGTGCAACAATCACTTGGGACATTGTTGTTGGAGGCTGGGACTTAGAATACAGTGCTGAGTTTGTTCCAAATGCTGAAGGAAGCTACACTATAGCAGTTGAGAAGCCGCGAAAGATTGGATCATCCGAAGAAGCGATCCACAACTCATTCACGTCGAAAGAATCCGGCAAAATGATACTCTCGGTCGACAACACCGCCTCAAGGAAGAAAAAAGTCGCCGCGTATCGTTATGTCGTCCGAAAATGCACCGCAACTTGAACGGATGCTATGGCCTCAAGTAGCTTTTAAATTTTNANTTTTTGCTAATGATTGTTTTTTTGTTTAGTACCTTTTGTTTTTATTGTTTTTTGTAACTTTGTTTTTTATGGCTA
TARGET=225,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0426
PRIMER_LEFT_PENALTY=0.024206
PRIMER_RIGHT_PENALTY=0.018372
PRIMER_LEFT_SEQUENCE=ATCCAACCTTGATGATGGATTC
PRIMER_RIGHT_SEQUENCE=GTCTTCTTCAAAGGAAGGGGTT
PRIMER_LEFT=191,22
PRIMER_RIGHT=339,22
PRIMER_LEFT_TM=60.024
PRIMER_RIGHT_TM=59.982
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=149
PRIMER_PAIR_PENALTY_1=0.0478
PRIMER_LEFT_1_PENALTY=0.024206
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=ATCCAACCTTGATGATGGATTC
PRIMER_RIGHT_1_SEQUENCE=GGATTTGAGAAGGGTGACAAAG
PRIMER_LEFT_1=191,22
PRIMER_RIGHT_1=366,22
PRIMER_LEFT_1_TM=60.024
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=0.0478
PRIMER_LEFT_2_PENALTY=0.024206
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=ATCCAACCTTGATGATGGATTC
PRIMER_RIGHT_2_SEQUENCE=AGGATTTGAGAAGGGTGACAAA
PRIMER_LEFT_2=191,22
PRIMER_RIGHT_2=367,22
PRIMER_LEFT_2_TM=60.024
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=177
PRIMER_PAIR_PENALTY_3=0.0782
PRIMER_LEFT_3_PENALTY=0.059875
PRIMER_RIGHT_3_PENALTY=0.018372
PRIMER_LEFT_3_SEQUENCE=ATTCCATCTGGGTTTCTCTCAA
PRIMER_RIGHT_3_SEQUENCE=GTCTTCTTCAAAGGAAGGGGTT
PRIMER_LEFT_3=168,22
PRIMER_RIGHT_3=339,22
PRIMER_LEFT_3_TM=59.940
PRIMER_RIGHT_3_TM=59.982
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=172
PRIMER_PAIR_PENALTY_4=0.0834
PRIMER_LEFT_4_PENALTY=0.059875
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=ATTCCATCTGGGTTTCTCTCAA
PRIMER_RIGHT_4_SEQUENCE=GGATTTGAGAAGGGTGACAAAG
PRIMER_LEFT_4=168,22
PRIMER_RIGHT_4=366,22
PRIMER_LEFT_4_TM=59.940
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=199
=
PRIMER_SEQUENCE_ID=SSR_05946 Ps_contig_mira-and-tgicl-ass_franssen_40692
SEQUENCE=AAAGGTGGCACTAATAGTTTCTTCATAATAATGTTGCAAATGGAACACATGTTACTGGAAATGTTAGTTCTATCAAAGGTCTTGTAGCATCAGATACAGGACCTGCATTAACTCCAGAGGCTTATAGACGTCGTCATGAGATCACTGTTACGGGAGATAATGTGCCTCCACCTGTTATGTCATTTGCGTCTTCTGGCTTTCCATCAGAGATTCTTAAAGAGGTACAAAATGCTGGGTTCTCAGCCCCAACTCCAATTCAGGCACAATCGTGGCCCATTGCCCTTCAAAGTAAAGATATAGTTGCCATTGCTAAAACAGGTTCAGGGAAAACCTTGGGGTATTTACTTCCAGCATTTATTCACCTCAAGCGCACTAATAATAACGCCAAAATGGGGCCCACTATATTGGTACTTTCACCAACAAGGGAGTTGGCAACACAAATTCAAGATGAAGCTGTGAAGTTCAGTAAAACATCAAGAATTGCCGGCACTTGTTCGTACGGAGGAGCACCAGAGGGTCCTCAATTGAGAGATATTGACCGCGGAGCGGATATTGTGGTAGCCACACCTGGTCGCTTGAATGATATTCTTGAGATGAGGAGAATCAGTCTACACCAGGTCTCTTACCTAGTCCTAGATGAGGCAGACAGGATGCTGGACATGGGTTTTGAACCTCAAATTAGGAAGATTGTGAACGGCGTGCCCGCTCGCAGGCAAACTCTTATGTTTACGGCAACTTGGCCTAAAGAGGTTAGGAAAATTGCAGCTGACCTGCTTGTCAATCCTGTCCAAGTGAACATTGGCAACGTAGATGAGCTTGTTGCTAACAAGTCCATTACTCAGCATAATTAGAAAGTTTTGGCACATATGGAGAAACAGCGGCGACTGGAAAGTATTTTGCGGTCACAGGATCAAGGATCAAAGATAATTATTTTTTGTTCTACCAAGAAAATGTGTGACCAACTTGCTCGTAATCTTACACGCCAGTTTGGAGCTGCTGCTATACATGGGGATAAATCTCAGGCTGATAGGGATCATGTGTTGAATCAGTTTCGAAGTGGAAGGACTCCTGTACTTGTAGCTACAGATGTTGCTGCTCGTGGACTGGACGTCAAAGACATTAGAGTGGTTGTCAACTACGACTTCCCTACAGGAGTGGAGGATTATGTTCATAGGATTGGAAGGACTGGGGAGGGCCGGAGCCACCGGTATAGCTTACACTTTCNTCGGGGACCAGGATGCTAAGCATGCTTCAGATCTTATCAAAATCTTAGAGGGTGCAAATCAGAAAGTCCCTCCAGAACTTCGTGAATTGTCATCCAGGGGTGGNCGGTGGGTTTGGCAGATCCAGACGATATGGTTCTGGTGGTCGTGGCGATTCTGGATTCGGTGCGAAGAGTTATGACTCTGGGTCTGGGTATGGTGGAAGGGGTAATGATTCAGGATATGGTGGAAGGGGTAGTGATTCAAGTTATGGAGGAAGGGGTGGATATGGGTCTTCTGCTGCTTTTGGCAGAGGTGGAGGCCGTGGATTTGACTATGACTCACAGAGGAATGAACGTGATCGAAGCCCTGACAGGGGATCTAGCTGGAGTGATCGCTTCAAAACTCGCGAGCGCAGCCGCAGTCCTGTCAAGGCTGCACCATCTCAATATAATGCCCCCGTCAGCTTTCATCAAGCAATGATGGAACGCAATGGAGGAGGCAGTGATCGCAACAAGAGCTTTAACCGGGAGCGAAGCCGTAGTCATAGTCCTTATAGACATGACAAGGGAAAACGTTCACCATCCCCCCCTGGCGGTGGTAGGACCAACAGTAGTTCAGGTGTTGGAAGAAACGGTGGTTCTCATCCTTACAATGGAGAATTGGAGGAAGGGATGATTCCTGAGGAAGAAGGAATGATAGGTCATGATGATTCTTCTATATATCAAAGTGCAAATTAGAATTTTGGCTACTTCTACTTTGACGATATGTTAAGGTAATTTTGGTCCACTATCAGTATTTTGAAAGACATTGGTACATGGACATTTGCAAGATATAGGATCTGGTAGTGGAATTAATGGGCAAATGTATATTTTGAGAAGCTAGGCTGATAGGTGAAGAACCTAGTAACAAATATGTTGATGGTTGCCTGATAGGTGAAGAACCTAGTAACAAATGTGTTGATGGTTTTATGTTTATTATTTTGCTAAAGGTTTAGTTTATATCTTCTACACAGCCAGACTAGAATTTGTTTAAGCCACTATGAAATGTTGCATCCATCGTTTTCTGATTGAGAGCTGCATCATTAGTTTTTTTGTTAAAGGAGCAGCGAACAAATGTGACTTTTATATTGATTTCATTGACAACATACCAGTGTTGTTTGTTTGTATTATGCACGAACTGCTATCGTGCTTCTATTATTATAATACAAAATCAATCAATTTTTTC
TARGET=2358,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8005
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.796144
PRIMER_LEFT_SEQUENCE=GTTGCCTGATAGGTGAAGAACC
PRIMER_RIGHT_SEQUENCE=TAGAAGCACGATAGCAGTTCGT
PRIMER_LEFT=2125,22
PRIMER_RIGHT=2397,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=59.204
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=273
PRIMER_PAIR_PENALTY_1=0.9265
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.796144
PRIMER_LEFT_1_SEQUENCE=TTGCCTGATAGGTGAAGAACCT
PRIMER_RIGHT_1_SEQUENCE=TAGAAGCACGATAGCAGTTCGT
PRIMER_LEFT_1=2126,22
PRIMER_RIGHT_1=2397,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.204
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.9974
PRIMER_LEFT_2_PENALTY=0.201304
PRIMER_RIGHT_2_PENALTY=0.796144
PRIMER_LEFT_2_SEQUENCE=GTTAAAGGAGCAGCGAACAAAT
PRIMER_RIGHT_2_SEQUENCE=TAGAAGCACGATAGCAGTTCGT
PRIMER_LEFT_2=2297,22
PRIMER_RIGHT_2=2397,22
PRIMER_LEFT_2_TM=59.799
PRIMER_RIGHT_2_TM=59.204
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=101
PRIMER_PAIR_PENALTY_3=1.0397
PRIMER_LEFT_3_PENALTY=0.243575
PRIMER_RIGHT_3_PENALTY=0.796144
PRIMER_LEFT_3_SEQUENCE=CAAATATGTTGATGGTTGCCTG
PRIMER_RIGHT_3_SEQUENCE=TAGAAGCACGATAGCAGTTCGT
PRIMER_LEFT_3=2111,22
PRIMER_RIGHT_3=2397,22
PRIMER_LEFT_3_TM=60.244
PRIMER_RIGHT_3_TM=59.204
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=287
PRIMER_PAIR_PENALTY_4=1.0528
PRIMER_LEFT_4_PENALTY=0.004359
PRIMER_RIGHT_4_PENALTY=1.048460
PRIMER_LEFT_4_SEQUENCE=GTTGCCTGATAGGTGAAGAACC
PRIMER_RIGHT_4_SEQUENCE=AATAGAAGCACGATAGCAGTTCG
PRIMER_LEFT_4=2125,22
PRIMER_RIGHT_4=2399,23
PRIMER_LEFT_4_TM=60.004
PRIMER_RIGHT_4_TM=59.952
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=275
=
PRIMER_SEQUENCE_ID=SSR_05947 Ps_contig_mira-and-tgicl-ass_franssen_40713
SEQUENCE=GTCGRNNGAATACATAACTGCTTCTCTCTACTTCATCAACCCAACCAACCAACCAAAAAACCACCATACCACCGTGGCTACCACCGCCGTGTAAGGCGGCGCGTGAGAAAGTTTGCGGCGCGTGAGAAAGTTTGCGGCCATATTTCCCTTACGTGCTGCTGATTGATCCACGCGGGAGGAGAAATGGGACAGAATCAATCAGGATGTGGGATGAAGAAGAAAGAGAGTGATTTGTTTGCTTCTGCTGTTGCCAATGGTGAGTTGGAGGTTGTTGAAGCTATGGTTGATGATGATGTGAATGTGTTGGATAGAACTATTGGTCGAGCTAGGTTGTCTCCTCTTCATTTAGCAGCTGCCAATGGTCGGATCGAGGTGCTTTCCATGTTGTTGGAGCAGAATGTTAAAGTTGATGTCTTGAATCGCCATAAACAGACGCCGTTAATGTTGGCTGTGATGCATGGAAAGACCGGTTGCATGGAAAAGCTTATTCAAGCTGGAGCTAATATATTGATGTTTGATTCTATTCGTCGAAGAACTTGCTTGCACTATGCTGCGTATTATGGTCATGTAGATTGCCTAAAGGCTATTCTTTCAGCTGCTCATTCCACGCCTGTTGCAGATTCTTGGGGATTTGCCAGATTTGTGAACATAAGAGATGGAAATGGTGCTACGCCACTGCACCTTGCTGCTCGCCATAGACGGCTTGAATGCCTTCATTCCCTTTTAGACAACGGGGCTCTTGTCTGTGCTTCAACCGGTGGATCCGGATTTCCCGGAAGAACACCACTACATATGGCTGCCCGTGGTGGTTCTGTGGACTGTGTCCGCATGTTGCTTGCTTGGGGAGCAGATCGCCTTCAATTAGATTCTTCTGGGAGAATACCATTCACGGTTGCTCTTAAGCACAGACACAGGGCATGTGCCGGCCCTACTCGATCCTTCATCAGCCGCACCGCTTGTTTGGCCGTCTCCGCTTAAAGTTTATCAGTGAGCTCAACCAGGAACGACCAAAACGTCCCTTTTAAGTTTAGGGGAACGGAAACCCGGTACCCTTTTAAACCTTAAAGGAAAAGGACCGTAAAACCGAAAGGGG
TARGET=41,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05948 Ps_contig_mira-and-tgicl-ass_franssen_40717
SEQUENCE=GTATCAACGCAGAGTAATATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATTATTATTATTATTATTCTTGTTATTATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATG
TARGET=18,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05949 Ps_contig_mira-and-tgicl-ass_franssen_40717
SEQUENCE=GTATCAACGCAGAGTAATATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATTATTATTATTATTATTCTTGTTATTATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATG
TARGET=38,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05950 Ps_contig_mira-and-tgicl-ass_franssen_40717
SEQUENCE=GTATCAACGCAGAGTAATATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATTATTATTATTATTATTCTTGTTATTATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATG
TARGET=59,27
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05951 Ps_contig_mira-and-tgicl-ass_franssen_40717
SEQUENCE=GTATCAACGCAGAGTAATATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATTATTATTATTATTATTCTTGTTATTATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATG
TARGET=92,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05952 Ps_contig_mira-and-tgicl-ass_franssen_40717
SEQUENCE=GTATCAACGCAGAGTAATATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATTATTATTATTATTATTCTTGTTATTATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATG
TARGET=116,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05953 Ps_contig_mira-and-tgicl-ass_franssen_40717
SEQUENCE=GTATCAACGCAGAGTAATATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATTATTATTATTATTATTCTTGTTATTATTATTATTATTATTCTTGTTATTATTATTATTATTCTTGTTATTATTATTATG
TARGET=137,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05954 Ps_contig_mira-and-tgicl-ass_franssen_40723
SEQUENCE=AACGGCGGCGAAGTTACATAAACCGTAAACGTGAGGGTTACATGGCATAGGAGGAAGTGGGCACCAAGCAACGGCGTTAACGTCGAAGAGATAAGGCGAAGCGATTGAAGGATCTTGAGGGAAGATACAGATAAGATTGTTACGATTTTGACCTAAGAGTGTGTTGAGAAATGATTTTGATGAAAGTGCGGATTTCCATGATTTGGAAGTTGATTTGAGACGAGCGTGATGAGCGTAAGGTACGATGGAGAGGATCGTTCTTGAAACTTCGGTTTGGGAGGCCGGGGATGAGGATTGATGACGATGGTGGTGGTGGTGGG
TARGET=305,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05955 Ps_contig_mira-and-tgicl-ass_franssen_40726
SEQUENCE=TRATAACTAGAAAGTTGTTCCACCCCTTTTCACTTTCTCATTCACTCTATCCACCATTCATCAATGGCAACCCTCATCAACCTTTCTTCTACGCAACTTCTCTCTTTCCACAGTTCAACCTCTTTTCATCCCCGAAATACCCCTACCCTTTCACTAAATTTACACCCCAACTCTTCTTCAAACCTACGCCCAATTTCCATCAAACCCTACCACCATTCCCGAAAGCCTCACACATGGCTGGTGACTATGGCAGTGAAAAACCTCGGCGAGACCGAATCGGTGACGGTGTCTCCGGAAAGCGACGGACCCGCCGGAGAATTGCCTCCGGAAGCAGGAGTTTATGCTGTTTATGACAAGGACGGCGAGCTTCAGTTCATCGGTTTGTCGCGTAACATTGCTGCCACTATTTCCGCGCACCGGAAGTCGGTTCCAGAGCTTTGTGGTTCCGTTAAGGTTGGGGTAGTGGATGAACCTGACAGAGAAGCTCTAACCCAAGCATGGAAGTCATGGATGGAAGAATACATAAAAATCACAGGCAAAGTTCCACCTGGTAATGAATCCGGAAACGCCACTTGGATAAAACAACAACCTAAGAAGAAGCCTGATCTTCGATTAACACCCGGTCGTCACGTGCAACTAACCGTCTCTCTTGAGGAGCTCGTCGACAAATTGGTTAAAGAGAACAAGGTAGTTGCATTCATCAAGGGATCAAGAACTGCGCCGTTATGCGGATTCTCACAAAAGGTGATAGGTATTCTCGAAAAGGAAGGAGTAGATTATGAGAGTGTGGATGTGTTGGATGAGGATTATAACTATGGATTGAGGGAAACTGTTAAAAAGTATAGTAACTGGCCTACATTCCCTCAGATATTTTTGAATGGTGAACTTGTTGGTGGTTGTGATATATTGACCTCCATGCATGAGAAAGGTGAAGTGGCTGGTTTGTTCAAAAAATAATTAACTGTGCAAGTGAACTTGCATTTTTAGGCATTCTTTTGAAATAATGAGGAACTAGCTAGCTAGGAGTAGAATGTGTGGAGCATTAAGGGAAGGAGAAATGCTGGTCATGCCTTGTTGTATTAATCTTTTAAGTACAAGGGTAGAAAGAAAATAATAATTATATGTATATTTTATTTCAACCATAGTAGAAAATGCTTTTTTTTTTTCTATATGTGCTGTATATTTGTTCTTTTACAGCCTANTTACAAA
TARGET=1012,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05956 Ps_contig_mira-and-tgicl-ass_franssen_40756
SEQUENCE=TAAATAAATCAAANAAACCCCAAAACTAATCTATCCTAAATCCTAATCCTCATCCAACCCATCACACATTTTCCCGTTTAATAACTCAATAAATCCAGTTATCCACAACTTAATCTCTTTTTCACTTCATTAAATAAATAAATAAGTTCACTCGTTCATATTTTCNGAGAAAATAATTGAATTTTCCTTAATTTTCTAAGCCTTTTCAATGGCGCTTCTATTACACTACCCTGAGGTTCACGTTGCTCTTCAATTCGCACAAAACCCTAACCCTAATTTGAGGAAACTTCACAGCTTCAAACCCTATGCAAAATTGAACGCAGTTGGAACAAAGAGCGGGGTGAAATTTTCCAACGAAGTAAAGGATTGTGCGGTTTTTGATTTTGATTTGGATTTGGATTTGAATAGGAACGTGAAGCAGTTTGGTCAGTTTTCGGTTCCGGTGAAGAGTAGTTCGCGGTCGAGTAAAGAGGAAGAGGAAGAAAAACAGAATTATTATGTGAATATGGGATATGCTATACGAACTTTGAGGGAGGAGTTTCCTGATCTCTTCTCTAAGGAACTTAGCTTTGACATCTACAGGGATGATATTGTGTTGAAGGATCCTCTGAATACGTTTATGGGTATTGAGAATTACAAATCGATATTCTGGAACCTACGATTTCATGGCAAGATATTTTTCAAAAGTTTATGGATAGACATAAATAGTGTATGGCAGCCGGTTGAGAATGTAATTATGGTTCGCTGGACCGTTCACGGGATCCCACGAGTTCCATGGGAAAGCCGAGGTAGGTTTGATGGGACGTCTGAGTATAAACTTGATAAACAGGGCAAGATTTACGAGCATCGGGTTGACAACATTGCTTGGAAAAAACCTCCTAGGTTTAAAGTTATGAGTGTGGAAGAATTGATTCAATCTATTGGCTGTCAGCAGACCCCAGGACCAACTTACTTCGAAATATCTTCATCTGCTAAGAGAAAGTAAAAGCTACTCCTGCCCTTCAAAAAGTTTTGGACCAAATTGTGCATATTATGGTATACATTAAGCTAGATATTTAGCATCTTGTTGTAGCAATAAACCCTTGAAGAAAATTGTTATTTTTCTTAGACCTAATNNTTATATAGTAAATAATCGGTTCCCATATCTACTGGCCAACCGTAAGGTTTTAAAAGGGGTTCTTTGGTTGG
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4151
PRIMER_LEFT_PENALTY=0.384836
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=AATCCTAATCCTCATCCAACCC
PRIMER_RIGHT_SEQUENCE=TGAAGCTGTGAAGTTTCCTCAA
PRIMER_LEFT=38,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=60.385
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.4151
PRIMER_LEFT_1_PENALTY=0.384836
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=AATCCTAATCCTCATCCAACCC
PRIMER_RIGHT_1_SEQUENCE=TTGAAGCTGTGAAGTTTCCTCA
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=299,22
PRIMER_LEFT_1_TM=60.385
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.4250
PRIMER_LEFT_2_PENALTY=0.384836
PRIMER_RIGHT_2_PENALTY=0.040146
PRIMER_LEFT_2_SEQUENCE=AATCCTAATCCTCATCCAACCC
PRIMER_RIGHT_2_SEQUENCE=AACGTGAACCTCAGGGTAGTGT
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=243,22
PRIMER_LEFT_2_TM=60.385
PRIMER_RIGHT_2_TM=59.960
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=206
PRIMER_PAIR_PENALTY_3=0.5359
PRIMER_LEFT_3_PENALTY=0.384836
PRIMER_RIGHT_3_PENALTY=0.151106
PRIMER_LEFT_3_SEQUENCE=AATCCTAATCCTCATCCAACCC
PRIMER_RIGHT_3_SEQUENCE=GGTTTGAAGCTGTGAAGTTTCC
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=302,22
PRIMER_LEFT_3_TM=60.385
PRIMER_RIGHT_3_TM=60.151
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=265
PRIMER_PAIR_PENALTY_4=0.5566
PRIMER_LEFT_4_PENALTY=0.384836
PRIMER_RIGHT_4_PENALTY=0.171757
PRIMER_LEFT_4_SEQUENCE=AATCCTAATCCTCATCCAACCC
PRIMER_RIGHT_4_SEQUENCE=AATTGAAGAGCAACGTGAACCT
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=254,22
PRIMER_LEFT_4_TM=60.385
PRIMER_RIGHT_4_TM=60.172
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_05957 Ps_contig_mira-and-tgicl-ass_franssen_40769
SEQUENCE=TTCTTGCAGGGGGAAATGCAGCAACTTCGTGACAAACTTGCTATAGTTGAGCGCAGAGCAAAGTCTGAAGCACAGTTGAAAGAAAAATATCAATTACGGCTTAAAGTGCTAGAGGAGAGTTTGAGGGGGAATTCTAATGGCAGTAATCGCAGCACCCCAGAGGGAAGAAGTGTGAGCAATTCTCGTCGGCAATCTCTAGGTGGAGCTGATCATTTCTCAAAACCAACCTCTAATGGGTTTTTGCCTAAGAGGTTACCATCCTTTCAATTAAGGCCATCTTTATCGTCTAGCGCAGTATTGAAGAATGCCAAAGGAACATCTAAATCTTTTGATGGGGGCACAAGGTCACTGGAGAGGAGTAAAATGCAGCTGAATGGAGCACCTCAAAGCCATTCTTTTAACGAATCTCTTGAAGAAACCAGAGAGAGAGAAGCAGATGTTAATTGGAAAGAAGATTCAGATGATAAGGCAAATGAATTCCCAACAGTGGATACAGAGGATAGTGTTCCAGGTGTTTTACATGATCTCCTGCAGAAAGAGGTCATGGCCTTGAGGAAAGCTGTTAATGAGAAAGATCAAAGCCTAAAAGATAAAGATGATGCTATTGAGATGCTAGCAAAGAAGGTAGATACATTAACCAAAGCCATGGAAGTTGAGGCAAAGAAGATGAGAAGGGAAGTAGCTAACATGGAAAAGGAGGTAGCTGCTATG
TARGET=421,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2493
PRIMER_LEFT_PENALTY=0.002623
PRIMER_RIGHT_PENALTY=0.246726
PRIMER_LEFT_SEQUENCE=TGGGTTTTTGCCTAAGAGGTTA
PRIMER_RIGHT_SEQUENCE=ATCCTCTGTATCCACTGTTGGG
PRIMER_LEFT=233,22
PRIMER_RIGHT=500,22
PRIMER_LEFT_TM=59.997
PRIMER_RIGHT_TM=60.247
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=268
PRIMER_PAIR_PENALTY_1=0.3628
PRIMER_LEFT_1_PENALTY=0.121299
PRIMER_RIGHT_1_PENALTY=0.241544
PRIMER_LEFT_1_SEQUENCE=CCTCAAAGCCATTCTTTTAACG
PRIMER_RIGHT_1_SEQUENCE=ATCTTCTTTGCCTCAACTTCCA
PRIMER_LEFT_1=381,22
PRIMER_RIGHT_1=667,22
PRIMER_LEFT_1_TM=60.121
PRIMER_RIGHT_1_TM=60.242
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=287
PRIMER_PAIR_PENALTY_2=0.3628
PRIMER_LEFT_2_PENALTY=0.121299
PRIMER_RIGHT_2_PENALTY=0.241544
PRIMER_LEFT_2_SEQUENCE=CCTCAAAGCCATTCTTTTAACG
PRIMER_RIGHT_2_SEQUENCE=CATCTTCTTTGCCTCAACTTCC
PRIMER_LEFT_2=381,22
PRIMER_RIGHT_2=668,22
PRIMER_LEFT_2_TM=60.121
PRIMER_RIGHT_2_TM=60.242
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.3628
PRIMER_LEFT_3_PENALTY=0.121299
PRIMER_RIGHT_3_PENALTY=0.241544
PRIMER_LEFT_3_SEQUENCE=CCTCAAAGCCATTCTTTTAACG
PRIMER_RIGHT_3_SEQUENCE=TCTTCTTTGCCTCAACTTCCAT
PRIMER_LEFT_3=381,22
PRIMER_RIGHT_3=666,22
PRIMER_LEFT_3_TM=60.121
PRIMER_RIGHT_3_TM=60.242
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=286
PRIMER_PAIR_PENALTY_4=0.3680
PRIMER_LEFT_4_PENALTY=0.121299
PRIMER_RIGHT_4_PENALTY=0.246726
PRIMER_LEFT_4_SEQUENCE=CCTCAAAGCCATTCTTTTAACG
PRIMER_RIGHT_4_SEQUENCE=ATCCTCTGTATCCACTGTTGGG
PRIMER_LEFT_4=381,22
PRIMER_RIGHT_4=500,22
PRIMER_LEFT_4_TM=60.121
PRIMER_RIGHT_4_TM=60.247
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.7000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_05958 Ps_contig_mira-and-tgicl-ass_franssen_40775
SEQUENCE=CTAGCATTAATCCTGTTGATTGGAAGATACAGAAGGGTGCACTTCGCGCAATGCTTATGCCGGCAAAATTTCCCCATATACCCTGCACTGATGTATCAGGAGAGGTAGTAGAGGTTGGACAGCAAGTGAAGGATTTTAAAGCTGGTGATAAAGTTCTTGCTAAACTATCAACTCAACATGGAGGTGGACTAGCTGAATTTGTGGTGGCCAGCGAGAGCTTAACAGCTGCCAGACCATATGAAGTTTCAGCTGCTGATGCTGCAGGTTTACCTGTAGCTGGTCTCACAGCTTATGACGCGCTCACAGAAATTGGAGGAATTAAGCTTGATGGGAGTGAGCCCAAGAATGTTTTGATAACTGCTGCTTCAGGCGGGGTAGGTCTTTACGCTGTTCAACTTGCCAAACTAGGAAGCAACCATGTGACAGCCACTTGTGGCGCTCGCAACATTGACCTCGTTAAGGGCTTAGGTGCTGACGAGGTTCTTGACTACAAAACTCCAGAGGGAGTATCATTAAAGAGTCCGTCTGGTAAGAAATACGATGCAGTGATAAATTGTGCCACTGGAATACGATGGTCAATTTTTGATCCTAATTTGGCTAAAAATGGAGTTGTTGTGGATTTAACACCTAGCGCAAGATCAATGTTTACTTTTGCCCTGAAGAAACTTACTTTTTCGAAGAAGCGGTTGGTGCCTTTTATTGTATCTATCAAAACCGAGGGCTTGAAACATCTTGCTGAGTTAGTGAAGGATGGAAAACTGAAGACAATTATTGACTCCAAGTTTCCTTTGAGCAAAGCTGAAGATGCTTGGGCTAAGAGCATTGATGGCCATGCTACTGGAAAAATCAATTAGTAAGAACCATAGGCACAGTTTACTTGGGAATGTGTGTGTGTGTGTGTTTATATAGAAATGAATAATCG
TARGET=885,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05959 Ps_contig_mira-and-tgicl-ass_franssen_40780
SEQUENCE=ATAAGCAATTGAATATCAACTTATTTATTATTCAAATAATTTAGTAGTTAGAATCTCACACAACATCTTGTCCAAATAATACAAACTGTTTCCAATCAAGAGTTGAGTATTAGGTACAAGAACTTACAGAGAAACATCAAGACAAAATAATTCAGGTTTTTATTCTGTTTGTTAGTCATATTAAACTCGACTTGAATCCCTCCCAAATACGAATCTTCTAGTCCAATCGGACACAACCAAATACCGTGTGCGCCAGCTCACTTGCTTGCTTGCATATACAGAATACCAAAGCCATTGAGTGCTGTGGCCAATGGAAACCCAGTCTCCAGGAAGTTCTGCTGCTGCCTGCTCCCCACCCAGTGGAGCAAATTGCCCAAAATGCTTATACCGGAAGGGACGAAACCGATGATGTCCAGATTCTGTAAATCGTCGAGGTCCTTCAGGATGCTCTTCACATTGATCATTGCGGTTAAAGCAACTAGCTAGATATGCACCTTGTTGCGCAGCAACCTGTGCAGTTGCTGGAAGAGTCTTCACTTGTGAATCTGCAACAGAAAGGGCAAGCTTAAACCCTTCTATGTCTATTTCTTTTCTTTCATTTCCTTCCGCGTCGTTCCACAGGACCCTCAAATCAAGTATATGTTTCTTCTGAAGATAGTATTCAACCTGAGGATATCTTAAGATTATGTCATCCATCACTTCTTTGAATTCTTCTACGGTTAAGGTCCCGGAGTTATTTTTGTCTGCGGCCTTAAATATGTCCAAGATATCATCCATGACTTTACGTTGCTTTATTGATGAGCAATCACCAATGGCGAATACATCTTCACACCCATTTACTCTCAACCATTCGTCCGTTGCTAGTACGTGCCTTTTGGTCTGACCGATTTCTTCCATAAAGTTCTTTATAACAGGAAGAGTAGAAATGCCAGTGGACCAGATAATCAATCCATGAGGCATAGAGCAAATCGCTCCGGTTGATTTCACTTTCATTGTAATTTCCTTGTCATTAACACTCAAAACCCGGCATCCAGTTTGAACCTCTATGCCATCTCTCGTAAACTTCTGCTCAGCAAATAAACTTATTCTTTCATCAAACATGTTCAGAATGTGATCTCCGGATTGAATTAACGTTATCTTCACTTTATCTTTAACAGTTGGATATAATTTAATCAAATCTTCTTGGATAAAATCATGAAGCTCTGCAGCAAATTCAACACCAGTAGGACCTCCGCCAACGATTACAAAATGAAGATTTGACCTTTGCTCCTCTTCAGATAGACTGGGAAGAACAGCCTTCTCAAGACAATCTATTACAGACAGTCTGATCTTCTGAGCATCCTCGACATCCTTCAGAAAATGACAATTCTCCTTGACACCCGGGGTATTAAAAGTATTTACTTGTGCTCCTACCGATACAACCAAGAAGTCGTAGTCCAGAGAAAACTCACCACTTCCCACTAAATTTTCAATATTAGACCGACAAAAAACCTTTTTGCCTGCAGCATCAATCTTGACACATTCTGCCTCCCAAAATTTGATTTCTCCTTTTCTCTTTTTAATGATGTTTCGGACGGGTTCTACGATGCTCCGAGCTTCAACTGTCCCACAAGTGACACTTGGTAATAAAGGAGTAAACGCAAAGTAGTTACGAGGAGAAACAACCTGAACATCATATAAGGATGCATCCAAACCCTTAAGGAAACTGGTAGCAGCCCATCCAGTTCCAAGGACAAGTACTTTCTTTTTGCGGGGCTCATTATCTTCAACAATAGGACGTTCAGGTTCTGATTGGGATTCTGAGAGTGCCACCACACCTCCACCGCTGATAGTACAGAGAAGAAGAAAGTTAGAGGAGAAAGGGTGAGTTGTGAGAGCCCTGGAGGCTTTCATGAAGAAGGAACCAATCGTCATGTTCACCCAAACGCCAACCGCTCCCACTTTTCAGCTNCGNNTTTGGATCCCAACTCCCCGAC
TARGET=261,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4346
PRIMER_LEFT_PENALTY=0.406861
PRIMER_RIGHT_PENALTY=0.027715
PRIMER_LEFT_SEQUENCE=TAAACTCGACTTGAATCCCTCC
PRIMER_RIGHT_SEQUENCE=AGCACTCAATGGCTTTGGTATT
PRIMER_LEFT=181,22
PRIMER_RIGHT=302,22
PRIMER_LEFT_TM=59.593
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=122
PRIMER_PAIR_PENALTY_1=0.4517
PRIMER_LEFT_1_PENALTY=0.406861
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_LEFT_1_SEQUENCE=TAAACTCGACTTGAATCCCTCC
PRIMER_RIGHT_1_SEQUENCE=TACAGAATCTGGACATCATCGG
PRIMER_LEFT_1=181,22
PRIMER_RIGHT_1=423,22
PRIMER_LEFT_1_TM=59.593
PRIMER_RIGHT_1_TM=59.955
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.8000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.5315
PRIMER_LEFT_2_PENALTY=0.406861
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=TAAACTCGACTTGAATCCCTCC
PRIMER_RIGHT_2_SEQUENCE=CAATGTGAAGAGCATCCTGAAG
PRIMER_LEFT_2=181,22
PRIMER_RIGHT_2=458,22
PRIMER_LEFT_2_TM=59.593
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=278
PRIMER_PAIR_PENALTY_3=0.5372
PRIMER_LEFT_3_PENALTY=0.406861
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=TAAACTCGACTTGAATCCCTCC
PRIMER_RIGHT_3_SEQUENCE=AAGGACCTCGACGATTTACAGA
PRIMER_LEFT_3=181,22
PRIMER_RIGHT_3=439,22
PRIMER_LEFT_3_TM=59.593
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.5372
PRIMER_LEFT_4_PENALTY=0.406861
PRIMER_RIGHT_4_PENALTY=0.130358
PRIMER_LEFT_4_SEQUENCE=TAAACTCGACTTGAATCCCTCC
PRIMER_RIGHT_4_SEQUENCE=AGGACCTCGACGATTTACAGAA
PRIMER_LEFT_4=181,22
PRIMER_RIGHT_4=438,22
PRIMER_LEFT_4_TM=59.593
PRIMER_RIGHT_4_TM=60.130
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_05960 Ps_contig_mira-and-tgicl-ass_franssen_40785
SEQUENCE=GTCGAGCGCCGTCAGGAAGACTCGTTCGTCCTCCTTGGTGCGTTCTCCTTCTTCGTTTGAAATCCGTCGAAGCCACCAGAATCTTCACAATATCAATGGTCGTCTCCATCTCGGTTTGGTGCGCACATCAACGGGGGAGATAAACTTCATTCTGAGATAAGCATGCAACTAAGCTTGTATCGAGGATTAAAGCGGAAGGGGGACGAAAACTCACCGGAATTCTACAACGGTGATGGAATAGGTAACGAGCTGCTTCGATTCTCGTTCACGTCTTCCTCCTGCTATTTCTGTTGCCTCTGCTATGAATAGTGGACGTCTTTCGAATCCGTGAGCAAAGCGTGAGTTCGTGTGCGAGAGAGTCCTGTTTATGGAGTATAGACCGAATCTCTCCTACTGTTCTTCGATCTTCTTCTTCTACACTTTCTTGAATGTAGAAAAAAAAAGTAGAGAAATGATTCAAGGTTGATGGAGAGCTTCGTTTTCTGGTTGGTGAAGATCATGGAATTCAGTTCAGTTCAGTGTTATTTGGATTGAAGGTTGAATTCCGTTGTTCTGTTAGGGTGATGGTGAAGGAGCTCAACAGAGTTGCAGGTTTGAGTTTCGACAGTGAAGAAGATTGGCTTTGGGCATTGTTGCTTTGGACGGTGAAGAAGATTGAAGCATATGGAAGAATCGAAACTCTATTGAAGAGTAAAAGGTGAAGTTGAAGATGAACCAATCTGTTGAAGATGTAGGAAGCTCCAGCCTTATGCAGAATGAAGGACACTCACGATTCTGAATTTTGAAAGGCATAGTCTTTTGGTTGGAAAGCTGCTTGGAATGTTTGTTTTGAACTGCTTTGGTAATTTGGTTTTGAG
TARGET=405,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0300
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.029253
PRIMER_LEFT_SEQUENCE=CGAAAACTCACCGGAATTCTAC
PRIMER_RIGHT_SEQUENCE=CATGATCTTCACCAACCAGAAA
PRIMER_LEFT=203,22
PRIMER_RIGHT=500,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=0.0300
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.029253
PRIMER_LEFT_1_SEQUENCE=ACGAAAACTCACCGGAATTCTA
PRIMER_RIGHT_1_SEQUENCE=CATGATCTTCACCAACCAGAAA
PRIMER_LEFT_1=202,22
PRIMER_RIGHT_1=500,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=59.971
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=299
PRIMER_PAIR_PENALTY_2=0.0780
PRIMER_LEFT_2_PENALTY=0.017846
PRIMER_RIGHT_2_PENALTY=0.060196
PRIMER_LEFT_2_SEQUENCE=TTTCTGTTGCCTCTGCTATGAA
PRIMER_RIGHT_2_SEQUENCE=AATTCCATGATCTTCACCAACC
PRIMER_LEFT_2=284,22
PRIMER_RIGHT_2=505,22
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=60.060
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=222
PRIMER_PAIR_PENALTY_3=0.0853
PRIMER_LEFT_3_PENALTY=0.017846
PRIMER_RIGHT_3_PENALTY=0.067436
PRIMER_LEFT_3_SEQUENCE=TTTCTGTTGCCTCTGCTATGAA
PRIMER_RIGHT_3_SEQUENCE=CTGAATTCCATGATCTTCACCA
PRIMER_LEFT_3=284,22
PRIMER_RIGHT_3=508,22
PRIMER_LEFT_3_TM=60.018
PRIMER_RIGHT_3_TM=59.933
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=0.1109
PRIMER_LEFT_4_PENALTY=0.017846
PRIMER_RIGHT_4_PENALTY=0.093026
PRIMER_LEFT_4_SEQUENCE=TTTCTGTTGCCTCTGCTATGAA
PRIMER_RIGHT_4_SEQUENCE=ACAGAACAACGGAATTCAACCT
PRIMER_LEFT_4=284,22
PRIMER_RIGHT_4=555,22
PRIMER_LEFT_4_TM=60.018
PRIMER_RIGHT_4_TM=59.907
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=272
=
PRIMER_SEQUENCE_ID=SSR_05961 Ps_contig_mira-and-tgicl-ass_franssen_40785
SEQUENCE=GTCGAGCGCCGTCAGGAAGACTCGTTCGTCCTCCTTGGTGCGTTCTCCTTCTTCGTTTGAAATCCGTCGAAGCCACCAGAATCTTCACAATATCAATGGTCGTCTCCATCTCGGTTTGGTGCGCACATCAACGGGGGAGATAAACTTCATTCTGAGATAAGCATGCAACTAAGCTTGTATCGAGGATTAAAGCGGAAGGGGGACGAAAACTCACCGGAATTCTACAACGGTGATGGAATAGGTAACGAGCTGCTTCGATTCTCGTTCACGTCTTCCTCCTGCTATTTCTGTTGCCTCTGCTATGAATAGTGGACGTCTTTCGAATCCGTGAGCAAAGCGTGAGTTCGTGTGCGAGAGAGTCCTGTTTATGGAGTATAGACCGAATCTCTCCTACTGTTCTTCGATCTTCTTCTTCTACACTTTCTTGAATGTAGAAAAAAAAAGTAGAGAAATGATTCAAGGTTGATGGAGAGCTTCGTTTTCTGGTTGGTGAAGATCATGGAATTCAGTTCAGTTCAGTGTTATTTGGATTGAAGGTTGAATTCCGTTGTTCTGTTAGGGTGATGGTGAAGGAGCTCAACAGAGTTGCAGGTTTGAGTTTCGACAGTGAAGAAGATTGGCTTTGGGCATTGTTGCTTTGGACGGTGAAGAAGATTGAAGCATATGGAAGAATCGAAACTCTATTGAAGAGTAAAAGGTGAAGTTGAAGATGAACCAATCTGTTGAAGATGTAGGAAGCTCCAGCCTTATGCAGAATGAAGGACACTCACGATTCTGAATTTTGAAAGGCATAGTCTTTTGGTTGGAAAGCTGCTTGGAATGTTTGTTTTGAACTGCTTTGGTAATTTGGTTTTGAG
TARGET=505,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1109
PRIMER_LEFT_PENALTY=0.017846
PRIMER_RIGHT_PENALTY=0.093026
PRIMER_LEFT_SEQUENCE=TTTCTGTTGCCTCTGCTATGAA
PRIMER_RIGHT_SEQUENCE=ACAGAACAACGGAATTCAACCT
PRIMER_LEFT=284,22
PRIMER_RIGHT=555,22
PRIMER_LEFT_TM=60.018
PRIMER_RIGHT_TM=59.907
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=272
PRIMER_PAIR_PENALTY_1=0.1265
PRIMER_LEFT_1_PENALTY=0.017846
PRIMER_RIGHT_1_PENALTY=0.108684
PRIMER_LEFT_1_SEQUENCE=TTTCTGTTGCCTCTGCTATGAA
PRIMER_RIGHT_1_SEQUENCE=CACCATCACCCTAACAGAACAA
PRIMER_LEFT_1=284,22
PRIMER_RIGHT_1=568,22
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=59.891
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.2798
PRIMER_LEFT_2_PENALTY=0.017846
PRIMER_RIGHT_2_PENALTY=0.261998
PRIMER_LEFT_2_SEQUENCE=TTTCTGTTGCCTCTGCTATGAA
PRIMER_RIGHT_2_SEQUENCE=AACAGAACAACGGAATTCAACC
PRIMER_LEFT_2=284,22
PRIMER_RIGHT_2=556,22
PRIMER_LEFT_2_TM=60.018
PRIMER_RIGHT_2_TM=60.262
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.3141
PRIMER_LEFT_3_PENALTY=0.221115
PRIMER_RIGHT_3_PENALTY=0.093026
PRIMER_LEFT_3_SEQUENCE=GTTCACGTCTTCCTCCTGCTAT
PRIMER_RIGHT_3_SEQUENCE=ACAGAACAACGGAATTCAACCT
PRIMER_LEFT_3=263,22
PRIMER_RIGHT_3=555,22
PRIMER_LEFT_3_TM=59.779
PRIMER_RIGHT_3_TM=59.907
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.3264
PRIMER_LEFT_4_PENALTY=0.249074
PRIMER_RIGHT_4_PENALTY=0.077340
PRIMER_LEFT_4_SEQUENCE=GTTGATGGAGAGCTTCGTTTTC
PRIMER_RIGHT_4_SEQUENCE=AACTCTGTTGAGCTCCTTCACC
PRIMER_LEFT_4=461,22
PRIMER_RIGHT_4=586,22
PRIMER_LEFT_4_TM=60.249
PRIMER_RIGHT_4_TM=59.923
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_05962 Ps_contig_mira-and-tgicl-ass_franssen_40787
SEQUENCE=TGTTCATTTCCTTTCCGCTCTTATTGCTTTTCTTTCACTTTTCTTCTTAGTTTAACAAGTTGGTCACCTCTCTCTCTCTCTCTCTCTCACATCTTGTTCTGTATTTCCATGGCCTCGAAGCGGATCCTGAAGGAACTCAAGGACCTTCAGAAAGATCCTCCAACTTCATGCAGTGCTGGCCCTGTAGCAGAAGATATGTTTCATTGGCAAGCAACTATAATCGGGTCCCCTGATACGCCCTTATGCGTGGGTAGGTTTGTTTTTTCTAGTTACCATT
TARGET=68,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1512
PRIMER_LEFT_PENALTY=0.088755
PRIMER_RIGHT_PENALTY=0.062402
PRIMER_LEFT_SEQUENCE=CATTTCCTTTCCGCTCTTATTG
PRIMER_RIGHT_SEQUENCE=ATCAGGGGACCCGATTATAGTT
PRIMER_LEFT=4,22
PRIMER_RIGHT=233,22
PRIMER_LEFT_TM=60.089
PRIMER_RIGHT_TM=59.938
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.2000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.2278
PRIMER_LEFT_1_PENALTY=0.088755
PRIMER_RIGHT_1_PENALTY=0.139083
PRIMER_LEFT_1_SEQUENCE=CATTTCCTTTCCGCTCTTATTG
PRIMER_RIGHT_1_SEQUENCE=ACTGCATGAAGTTGGAGGATCT
PRIMER_LEFT_1=4,22
PRIMER_RIGHT_1=173,22
PRIMER_LEFT_1_TM=60.089
PRIMER_RIGHT_1_TM=60.139
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.3208
PRIMER_LEFT_2_PENALTY=0.088755
PRIMER_RIGHT_2_PENALTY=0.232060
PRIMER_LEFT_2_SEQUENCE=CATTTCCTTTCCGCTCTTATTG
PRIMER_RIGHT_2_SEQUENCE=ATATCTTCTGCTACAGGGCCAG
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=196,22
PRIMER_LEFT_2_TM=60.089
PRIMER_RIGHT_2_TM=59.768
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=193
PRIMER_PAIR_PENALTY_3=0.3254
PRIMER_LEFT_3_PENALTY=0.263023
PRIMER_RIGHT_3_PENALTY=0.062402
PRIMER_LEFT_3_SEQUENCE=GTTCATTTCCTTTCCGCTCTTA
PRIMER_RIGHT_3_SEQUENCE=ATCAGGGGACCCGATTATAGTT
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=233,22
PRIMER_LEFT_3_TM=59.737
PRIMER_RIGHT_3_TM=59.938
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.4021
PRIMER_LEFT_4_PENALTY=0.263023
PRIMER_RIGHT_4_PENALTY=0.139083
PRIMER_LEFT_4_SEQUENCE=GTTCATTTCCTTTCCGCTCTTA
PRIMER_RIGHT_4_SEQUENCE=ACTGCATGAAGTTGGAGGATCT
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=173,22
PRIMER_LEFT_4_TM=59.737
PRIMER_RIGHT_4_TM=60.139
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_05963 Ps_contig_mira-and-tgicl-ass_franssen_40794
SEQUENCE=CGCTTATGGACAGACGAAGACGAGATGGAGCTCTTGCAAGGTTTCCTCGACTACACAACGCAGAGAGGATCTTCTCACCACAACGACACAGCTCAATTCTACGACCAAATCAAATCCAAACTCCAACTCGATTTCAACAAGAATCAACTCGTTGAGAAGATCCGAAGGTTGAAGAAGAAATACCGAAACGTTCTGAACAAAATCAGCTCCGGCAAGGAATTCGTCTTCAAAACCGCTCACGATCAAGCTACTTTCGAAATCTCGCGCAAGATCTGGAACAACGTCGCGCCGGTTCCAGGTGATGTTGCCATAGACGACGACGAAATCAACCTGAATCCAAGTCCGAACCCTAACCTCAATTTCAGTCCGGTAATTCTCAAGGCCGAGGCGAATTCGAACACGAGGAAGCGTTCACGGCCTCGATCCGTTATCAGAACGTTCGAGAAACGCGAATCTATTGATTTATCAATTAGAGATCGTGATTATCTCAATATCAACCTCAACAATCCTAATCCAAATCCTAATCCTAATCCTCTTCCAATTCCTATTCCTATTCCAATTCCTCATCCTAATCCTAATCCTTACACTACTACTACAGCTACTGTTAATGCCGCTATAACACCTACTATTACTACTACTACTACTCCTAATGCTAATACTGCAATTAATGCTAATAATTATAGTAATAATTGCAGTAGCATTGGGAATAATGTTGCTGGTGTGATTGAAGAAACAGTGAGGAGTTGTTTATCGCCGGTGTTGAAGGAATTGGTAGCTGGTGCGGGTGCGTCTGCGATGGGAGGATCGGTTGGAGGAGCGCGAGGAGGCTTCGGTTTCTCGATGAATCCGGTGCCGTTTCAGTTCATGAATTTCGGTTTCGGAGGCGGCAGCGGCGGAGAAATTGTGGATGAGAAATGGCGGAAGCAACAGATTTTGGAATTGGAAGTGTATTCGAAGAGATTGGAATTGGTTCAGGATCAGATCAAAGCATCTCTTAACGAGTTGCGATCAATTGGTGGAACAGGAGAATGAAAATTCAGCTTTTAGTTAATTATTGTTTTAACTTAGTGATTAGTTTAAGTTATTGTAATTCTTCTCTTCATGTTCTCACTTCATTATACAGATTTGCTTCTTAATTTGTCCACCGATATGGTTGATGAATGATGATACTCATAGTTTAGCAGCTAGTTATTTTCTTTTCTTTTTTTGTGTTTAATTCGAATAGCATTTGTGTTTAATAAAAAAATAGTANTTTGG
TARGET=630,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7673
PRIMER_LEFT_PENALTY=0.729992
PRIMER_RIGHT_PENALTY=0.037339
PRIMER_LEFT_SEQUENCE=TTCCTATTCCAATTCCTCATCC
PRIMER_RIGHT_SEQUENCE=CTTCAATCACACCAGCAACATT
PRIMER_LEFT=547,22
PRIMER_RIGHT=729,22
PRIMER_LEFT_TM=59.270
PRIMER_RIGHT_TM=60.037
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.7673
PRIMER_LEFT_1_PENALTY=0.729992
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=TTCCAATTCCTCATCCTAATCC
PRIMER_RIGHT_1_SEQUENCE=CTTCAATCACACCAGCAACATT
PRIMER_LEFT_1=553,22
PRIMER_RIGHT_1=729,22
PRIMER_LEFT_1_TM=59.270
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=177
PRIMER_PAIR_PENALTY_2=0.8840
PRIMER_LEFT_2_PENALTY=0.729992
PRIMER_RIGHT_2_PENALTY=0.154023
PRIMER_LEFT_2_SEQUENCE=TTCCAATTCCTCATCCTAATCC
PRIMER_RIGHT_2_SEQUENCE=TTTCTTCAATCACACCAGCAAC
PRIMER_LEFT_2=553,22
PRIMER_RIGHT_2=732,22
PRIMER_LEFT_2_TM=59.270
PRIMER_RIGHT_2_TM=60.154
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=180
PRIMER_PAIR_PENALTY_3=0.8840
PRIMER_LEFT_3_PENALTY=0.729992
PRIMER_RIGHT_3_PENALTY=0.154023
PRIMER_LEFT_3_SEQUENCE=TTCCAATTCCTCATCCTAATCC
PRIMER_RIGHT_3_SEQUENCE=GTTTCTTCAATCACACCAGCAA
PRIMER_LEFT_3=553,22
PRIMER_RIGHT_3=733,22
PRIMER_LEFT_3_TM=59.270
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.8840
PRIMER_LEFT_4_PENALTY=0.729992
PRIMER_RIGHT_4_PENALTY=0.154023
PRIMER_LEFT_4_SEQUENCE=TTCCTATTCCAATTCCTCATCC
PRIMER_RIGHT_4_SEQUENCE=TTTCTTCAATCACACCAGCAAC
PRIMER_LEFT_4=547,22
PRIMER_RIGHT_4=732,22
PRIMER_LEFT_4_TM=59.270
PRIMER_RIGHT_4_TM=60.154
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=186
=
PRIMER_SEQUENCE_ID=SSR_05964 Ps_contig_mira-and-tgicl-ass_franssen_40795
SEQUENCE=GGAAGGGAAACGGTACCGAAATACTAGAAGGACGATCAAGGTTAGGTCGTGTCGGGAGTAAGAGTTTATTACTACAAATAGACAGATCTGCAGAAGTAAGAAGYTAATCTCAATGACACTTCATGCGTTTCGGACGAAAATGGGGCTGTAATGTCATCACTCAAGAGTCACGCACATTTGTCCAAGAAAGAAGCGGAAATACCAGTAATGGATAATAAAGGCGATGATGGCCGACTCAGTGGTGTTTTAACTGAATTATCATCATTGAAAGAAAGAGAAAACTCAACGAAAACCGAACTAAAAGAGTTGCAAGAAAGATACTCAGAAATGAGTCTCAAATTCGCAGAGGTAGAAGGCGAAAGACAAAAGCTTGTCATGACAGTAAGAAGCCTCAAGAATGCTAGGAAGGGCTAAAGTTTTCTTCCTGCTATTGTATAGTTGCAGCACTGAAACAGTTTCAAATGAATAATTTAATGTGTGCCAAGTTATATATATATATATACATGTTATTCCATTTTGTAGAGAGTTATCAATAGCTAGTTTGACAGTAGTTTAGTTAAAGGTTTAAAATTATTGTTTAGTCACCAATAG
TARGET=487,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05965 Ps_contig_mira-and-tgicl-ass_franssen_40800
SEQUENCE=CGGGGGTCTCCGAATACAGCTCAATCCAACAAAAACTTCCAAACACGTCTCAAACAAAGCTCAACCGGAGAAGTTACCTCCACCGCAACGGAAATTGGCTCCGGCGGCGGCGGAGGGCCAAATGCCTAACCTCCTACATCAAAATGTTCGTATCTTCCTCCCCTACCTAAATTTAAACTCCTTTTGTCCTAATCCGATCTCAAACTTCCAAATCGAGCCCTACCATATATGAACCGTAGAACTCTAAAATCAAATTAAACCCAACGATGCTTAATTCAGACTCCAAAGTTTAGGGCTATGAATCCTAAGCGCTCGTAANTACATGCTGGTAATGAAAATTGAAGATGAACAGAA
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1943
PRIMER_LEFT_PENALTY=0.013401
PRIMER_RIGHT_PENALTY=0.180917
PRIMER_LEFT_SEQUENCE=GCTCAATCCAACAAAAACTTCC
PRIMER_RIGHT_SEQUENCE=TATATGGTAGGGCTCGATTTGG
PRIMER_LEFT=18,22
PRIMER_RIGHT=228,22
PRIMER_LEFT_TM=59.987
PRIMER_RIGHT_TM=60.181
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=211
PRIMER_PAIR_PENALTY_1=0.2159
PRIMER_LEFT_1_PENALTY=0.013401
PRIMER_RIGHT_1_PENALTY=0.202525
PRIMER_LEFT_1_SEQUENCE=GCTCAATCCAACAAAAACTTCC
PRIMER_RIGHT_1_SEQUENCE=GGTAGGGGAGGAAGATACGAAC
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=166,22
PRIMER_LEFT_1_TM=59.987
PRIMER_RIGHT_1_TM=60.203
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.2159
PRIMER_LEFT_2_PENALTY=0.013401
PRIMER_RIGHT_2_PENALTY=0.202525
PRIMER_LEFT_2_SEQUENCE=GCTCAATCCAACAAAAACTTCC
PRIMER_RIGHT_2_SEQUENCE=AGGGGAGGAAGATACGAACATT
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=163,22
PRIMER_LEFT_2_TM=59.987
PRIMER_RIGHT_2_TM=60.203
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.2229
PRIMER_LEFT_3_PENALTY=0.041981
PRIMER_RIGHT_3_PENALTY=0.180917
PRIMER_LEFT_3_SEQUENCE=ACACGTCTCAAACAAAGCTCAA
PRIMER_RIGHT_3_SEQUENCE=TATATGGTAGGGCTCGATTTGG
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=228,22
PRIMER_LEFT_3_TM=59.958
PRIMER_RIGHT_3_TM=60.181
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=8.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.2229
PRIMER_LEFT_4_PENALTY=0.041981
PRIMER_RIGHT_4_PENALTY=0.180917
PRIMER_LEFT_4_SEQUENCE=AACACGTCTCAAACAAAGCTCA
PRIMER_RIGHT_4_SEQUENCE=TATATGGTAGGGCTCGATTTGG
PRIMER_LEFT_4=41,22
PRIMER_RIGHT_4=228,22
PRIMER_LEFT_4_TM=59.958
PRIMER_RIGHT_4_TM=60.181
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_05966 Ps_contig_mira-and-tgicl-ass_franssen_40817
SEQUENCE=GGGAGGACTATAAACAGGAGAAGGTGTTTTGGGAGGTGTAATTGGAACATAAGGAGGTGTAGGAAGAAGCTTGGGAGGAGGAGTAGACACATAAGGAGGAGGTTCTCTCACAGCCAACACCACAACAATCAATTTTTGACCATTTTCACAATTATGATCTTTTCCACTAATGAAATAAAAGGGACCAGATCTATCAAATTGAAACTCAGAATCACCATCTTCAAATTTCTTGATCGGATTCTTCTTATTGCACTCATCATAATCCTCTTTGTTCACCACCAACACTGAGTTTGACTCCTTCTTGTACTTAAAAACTAGAATATCATTGACTTGGAACCTATTCCTCGCAGTCCATTGATTGTAACTTTCAGAAGGGTTCAAAACCCATCCATCTTTTCCACCAGCATAAAACGTATAGCCTTGTGAACATGAAATTAAACTAAAAAAGAAACAAGAAGATGCAAATATGTTTTCGAAGAAGCTTCATGACTAATTAACAAGTACTATACATAGAGAGAGAGAAGAGAGTTAT
TARGET=512,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05967 Ps_contig_mira-and-tgicl-ass_franssen_40827
SEQUENCE=GAAACAACATGGTTGATAGTGAAGGCGATGAAGATTCCGTTACTTAACTCAGTTACTTGGACCGCTAGAATTGGCTGTGACGTGGATTCGTGGTTTCTGACGCCGTTAAGTGGGAAAAAGGAGTGAACAATGGAAGGTATGTAGTTGGGTTGAAGGATGTCGGAAACGGTTGTGGTTTCTGCTTTGGYATGAACGAAGAGAGCACCAACGTTGTTGCAAATGATGGAACATGAAGCGTTGTTGTTGTTGTGTTGTGTGATTATGAGACCGGYCYGTTAAGGGCGGAAAGAAGGAGAGTGTGGTTGAAAGAGTTTGTTTCAAATGATTGATTTGGTTGGAAGTGAGTGGCGGGTTGTTAGAAAAAGAAGTACCTTTTGGATTGGTTTCAAGGTTGGAGGAACTGAAGATCC
TARGET=237,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05968 Ps_contig_mira-and-tgicl-ass_franssen_40838
SEQUENCE=TTGGTTTCAAATTATCTATTCAATCTAGAAATCAAACAGCCCAACACAAAACAAATAGAGCGCAGTAAATCCCCAGAAGAACACAAACAGAAGGATGCAACCAAATCAAAATGCGACAAATACAAACCAAAATCGCATAGTACAATTGATTTTTAAGCCAAAGAAGACGAACCCTATGACGTCGAAGATGCAAGCTGGTATCGTTGTACTCGTCCANTTGAGGTCGTCGTTGAGACTCGCTGAGTAGTGTTGTCAGTGACGGATGTTGTTACCCTATCGCGTTTACTGTGTTATGCGATTTGTGCTTCAAACAGGAACAGAATAAAATTCTCAAAATCCTATTCCAATTTGGAACCACGTACATAACAATAACGGCGGACACGGGTTGGTGCGAGCGGATTTGACGGCGGTGTTTACAGAATAGTGGAGATTGAAATTGGAAGGTGTTTGTTGGTGATGGAAGAAAGGCGACGGTGGTTTGGGTTTGTGAGGGAGGTGGGTCTTACGGCGATGACGGAAGGGTGGATCGCGGAGGTTGTGTGAATCAGAGAAGGACGGCGGATGGGTTTGTGAGGTGATTTTCGGTGATGGTTTTGTTGATTCGTGGGTTGAATGAAGGTGAAGATGGAGTGAGGGTTTTGATGGTGGTAGTTGGAATCGAGGAGGGCGATTGAGGGTGTTGATGGTGGAGACCGAAATGGTTGAAGATGAAGGTGAAAGAGTTTATGGCGGCGGAGAAGGAGAAGAGAGACGAGGGTTTTTGTGGTTTCTGTGAGTGTGAGGTTTGTGAGAGTGATTTATGAGGGAGAGGTGTGGAGAATGAGTGAATGAGAACCAGCTGAGGAATTCTGTGAGAGAGTGAGTGAGTTGGCGTGAGGGAGTGTGCTGTCCCCGA
TARGET=857,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05969 Ps_contig_mira-and-tgicl-ass_franssen_40844
SEQUENCE=TTAAATTTGTTTTCTCTAGTGACTAAATAAATGGAATTAAATACATGAATTAAAATAGTGTTCCACTTTAACGATGGCTAATAATAATAATATTGCCATTATATTCACAAAACTAGTGAAAAGGGGCTACTATACTAATTCTCTCAGGAGTAAAGTTTGCTCCTGCATTAACATGCTGCAGGCCTGCACAAGAATTTCTGTTGTTGCCCATGGCATTGAACATGACACGACCTCGCTTTATCTCTCAATTCTTGGAATGCTCTCATTGTATCAGCATCGAACCCGCTAGCAAACTGATGTACCCGGAAAGCAAATCTTACTCCTTGAACAATCAGTTCTTCTTCTTCAGGACCACCACCAACTGTCTCAAGCTCAGCAGAGACTCTTTTCATATACTTCATAGCCAATTTTACAGATGCCAGCTTGATCTTGGTGGCATAACCGCTATCGAGCAACCAATCTATAGGAATTTGGAAGACTTTGAATCTCTTCGTTGCTGACTCCCTCATTCTAGAGATATTGTAAACCCCGTGTTCTAATTTCTCAAATAGAGTCTGCATCTTCTTAAGAGCTGGACCACATAGTTGTCGAGGATCGTCGCGGAAAGACGAAGCCTCGGATTCAAGCTTCTTCAGATCACAATATCCAAATGCAGCTTCACGCAGAGCATCAGCTTTCTGCYCTGGCCAATTGAAGTGTTTGAGCA
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05970 Ps_contig_mira-and-tgicl-ass_franssen_40844
SEQUENCE=TTAAATTTGTTTTCTCTAGTGACTAAATAAATGGAATTAAATACATGAATTAAAATAGTGTTCCACTTTAACGATGGCTAATAATAATAATATTGCCATTATATTCACAAAACTAGTGAAAAGGGGCTACTATACTAATTCTCTCAGGAGTAAAGTTTGCTCCTGCATTAACATGCTGCAGGCCTGCACAAGAATTTCTGTTGTTGCCCATGGCATTGAACATGACACGACCTCGCTTTATCTCTCAATTCTTGGAATGCTCTCATTGTATCAGCATCGAACCCGCTAGCAAACTGATGTACCCGGAAAGCAAATCTTACTCCTTGAACAATCAGTTCTTCTTCTTCAGGACCACCACCAACTGTCTCAAGCTCAGCAGAGACTCTTTTCATATACTTCATAGCCAATTTTACAGATGCCAGCTTGATCTTGGTGGCATAACCGCTATCGAGCAACCAATCTATAGGAATTTGGAAGACTTTGAATCTCTTCGTTGCTGACTCCCTCATTCTAGAGATATTGTAAACCCCGTGTTCTAATTTCTCAAATAGAGTCTGCATCTTCTTAAGAGCTGGACCACATAGTTGTCGAGGATCGTCGCGGAAAGACGAAGCCTCGGATTCAAGCTTCTTCAGATCACAATATCCAAATGCAGCTTCACGCAGAGCATCAGCTTTCTGCYCTGGCCAATTGAAGTGTTTGAGCA
TARGET=336,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05971 Ps_contig_mira-and-tgicl-ass_franssen_40845
SEQUENCE=CCGTACCCAACCTTTACCCCCTTCGGTTTCCCCCTTCCCTTTCCTTTTCCTTCCCCTTCGGGAAACCGAAAAAACCCTAACCTTCTTCTYCAAGAATGAAGAACTCACCACACTTTTCTCCAAAGAAAAAACCCAACAAGAAACATACTACGAGGATCTGAAAAATGTCGTGGATTTTGTTTCTCTCTCTCAACCTCGTCGTGAAGCTGTTCAATGGATGCTTAAAGTCAATGCTCATTACGCCTTTTCACCTCTCACTGCAACACTCGCTGTTACTTACTTTGATAGGTTCCTTCTAACCTTCCATTTCCAAAAGGATAAGCCATGGATGATTCAGCTTGTTGCTGTTACTTGCATCTCTTTAGCTGCTAAAGTTGAAGAAACTCAAGTTCCTCTCCTCTTAGACCTACAAGTGCAAGATACTAAATATGTGTTTGAAGCAAAAACTATTCAGAGAATGGAGCTTTTGATTCTGTCAACACTGAAATGGAAGATGCATCCTGTGACACCACACTCTTTTCTAGATCATATAATAACAAGGCTTGGTTTGAAAACTAATCTTCATTGGGAGTTTTTAAGACGCTGTGAGAATCTTCTTCTATCTGTACTTTTAGATTCAAGATTTGTTGGTTGTGTTCCCTCTGTGTTGGCTACTGCTACAATGCTGCATGTGATAGACCAGATTGAAGAGAGTGATGATAATGGCGTGGACTACAAAAATCAGCTTCTTAGTATTCTCAAAATCAACAAGGAGAAAGTGGATGAATGTTATAATGCTATTGTTGAGGTTACTAATGAAAATAATTATGGTCATAAACGAAAATATGAACAAATCCCTGGAAGTCCAAGTGGCGTAATTGATGCTGTTTTTAGTTCTGATGGTTCCAATGATTCATGGAAAGTGGGTTCATCCTCGTATTCAACCTCAGAGCCTGTTTTTAAGAAAACAAAAACTCAAGGGCAAAATAGGAATTTGTCACCTCTTAATAGGGTCATTGTTGGAATTCTTGCCACTGCTAGTGCTACTACCTCTCCTTAATATCCTCTCTCTGTCCTTTATAAAAAAACAAATAATAACCATATGAAAAAAATCTATATTTATTTAGTATATATGGTTATGTTGATGTTTCTAAGATCTCTTCATTTCCAACAATGTCCCTAATGAATCTCTTGAAGAGGCAGAGACAGAGAAAGAGGCAAGGTGAAAGGAGTGAAAGAGGAGAGGACAAAGTTGGCAATGAAGATTATGTTTTTTTTTAATTTGAAGGCACTTTTTATTTACTATTGAATGGTTGTTCTCTTCTAGACCATACCAAATTTGAACATATTTATATGATATTTCTATAATAAATTGGGAATAATTTTTGTTTCATATGTATGCTTCTATTTTNATTCACTTGTTTGAATCTTTGAATGAATGATGATAATAAG
TARGET=182,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05972 Ps_contig_mira-and-tgicl-ass_franssen_40860
SEQUENCE=CCTGAATACTAGAAGTGGAGCTGTAAACAAAATACCTCCRACTACAAATTTCTGAACAAGTCAGAGGATTAGTAAATGTTCAACTAGAATCAAGAGTTTACACCCAAAAAGAAATTCCAAGTACATGTACAGATTCTGCCAAAAGATTAAGTGGGGGTGCTTTGATATGTAAAATGACATTGTTCATAAACTTTACAAGAAATTCTTTACTTCACTGCATTGATGTGGATGCAGAAGGTATGAATCTCATGAGGAAGCTTGACAGGGACCATATGCAAATCACACCAAGGAATTGTAGGACATTTAGAAGGGTACTCAAAGAGCTGTTTATAGCCATGTTGTGTGGGGTCAACTCAGCTCCTCTAACTGTAAATGACAAAGCTGTAACCAGTTGTGCTGCTCTATTTAGTTGATGCAACCTATACACTTGAAAGAGTAATGGAATTGCAGACCATGAAGGTGACTTCCGATATTTAGCATACTGCTCAAATGCAAATTGGACAACTATGCCAACCAAGTATGGAGCAAGAGTTGCAGAGGCTGCAGGGCCTGTCCATGGCCAGACCAAACCCATAGTGACCATGGGAATTACAAGACTAAGTACTAAGGTAGCAACAGAAGAGATTCTCTGTCCCAGTTGAGGGGGCAGAGTATTAGTATTTTTAACCGCGGTATCCAACTGTATTGGTCTCCGTAAATGATCCAGTAGAACAAGAAACGCAGCAACACCGCCATAGAAAATTGCTTCCGTGAAGAATAATATGAGGAAATCTGTTAACAGAGAGTCCTCAAAAATGATGGAAAGCAATTTCCGCAAATAGAGTGAAGGAAAAATGAAAGAAGCCACTAGGACTGCAGGGCCCACAAACCACAATATGCTATTTTGACTCCTCTCTGGATTCGAGAAAAGAATCTCACCATCTCTTCTGTATGGGCGGTTATAAAATGATATTAAACCTGGTTTTCCATCACTCCCTGATAATTGAAAAGATGGGTTGCTAGCAGGAGAGATATCTTGAGCAATATCATCGTTGTTTTCTGGCACTGAAGCGTCTATTTTAAGCTCATTGTATTGTGACTGATCCTCGGAGACAACAAAAGGAGCATTTTGTTGTTTCTGGAACCTCCCTTTTAACTGCAGCCTTTCGTTCGTAACTTTATACTGGCGCGATTTGTCTAACCATGATACTTTAGTGTATATCCGTAGCCGAGAACTCGTTATCTGGTTGAGAAGCAACCCTTGTGATAAGCTCAACTGACGGTGACGAGGAGAAGAGCGTTGAAAAGGAGAAGGCGGTAAAGAATGAAATGAGGAAGCGAGTGAAGAACTGGTACTAGTAGTATTCGTATTTGCCATAACTATGTATAAACTAACAACTCAACTATATATCTGATGATGATGGTTCTGAAAGAAGCGACAAGAAGAAGAAGAAGAAAGAAGAAGTNNGAAGAAGTAAGTAGTANAANAAAGAAGTAAGAGTAAAG
TARGET=1420,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05973 Ps_contig_mira-and-tgicl-ass_franssen_40861
SEQUENCE=CGCCAGTGTGAACAAATGAACAAATAATTATAAAAACCTGATTTTGTTACAACATGATTAGGCAAATAAAAAAAACATGCCTAAAACAATGCAACCCAATTAGTGGAAACCCAATTACAAGTATGAAAATGAAATCACACCTTAAACAAAACAAGCACACACGTATACTCACAACAAGAATCTGATAAAACTCACTTTTGTTGGAAAAACTAAGTTAATTTTGCTGCTACACTAGATCATCTGGCCCAGCAAGCACAACGTTGCAATCAAGAGCGTGTTTCCTCGCGATCATAATGGCAACATTTCTTTCTTTCTCTGATTCAAAGGTTAACACAAATGACAGCCCTTTTCTTGCTTGCCAAAATAACGCCTTTGCTGCATTACCGACATCACTTCTGACTCCGCATAGCTGCATTGTTGGGGAGTAAATCTCTCTAGCTTTTGTAATCCAACCCCTACATAGCTTTATCCTCATTCTACCCACATTGAATGAATGAGTAGAATGTGATGAGTGATCTTTTCCATTCATCTGAGAAATAACTACATTAAAATCAGTATTGGATTTTCGGAGAAGTGTTTCCACATGGCTTCCGAGTCCTGCGACAGTTTGAATGGGATTAGTTGTTAGTGTGAGTTTCTTGCCATTGGATACAATGTCCACTTGTAACATTCTCCCAACATCAAGGGGATCAGGAGCATATATTGATTTGTCAGCACCTGAAATAACCTCCCTCCAGCTGCCTTCAGATGACAGGCGATACCACTGAAAGGAACATTTTGAAACCTGCAGCACTTCATTCGAGCACGGCTTGACTTGCAAGCATGAACCCAAAGCTTCAGAACCGTCTAACTTATATGAATGAGATTTATTCTCCTTGTCCCACTTGCTCATTGTTAGCTCTTTCTGAAGCTGAATTGAAAATATAGATTTTTCTTGAATTTGATCCCTTAGAGCACGAAGTTCATATTCCATGGCCATAACCTTGCTTGGTTGATGTAGCAATCTGATTCTTCTAGCCTCTTGAACCTCTTCTACGAGTCCATCCACATCCTGTGGTTTAGAAGCTTGGATAGCTTTTTCTTGTTCCTCAAGTGCTTCGCCGATTCTCAGCACCACTGCTCTAGCACTCTCGATTTCGGCACAGGCAAAAGACTTCTCTTGATTAACCAGCTTTTTAGCATCTTCAGAAGCCTGTTTGAGAAAGTTCACAAGCTTTTTCACTTCAAACTTCTCTTGAATCAGTTCTCCTTCATTCTGAGTCAACTTAACAGCCAAAGCTTCCACCATAGAGATAGCTTTCTCCACATCCTCTTTATTTCTTCCTGTAAAACGGCCTTTCAAAGATTCTAATGCATCTCTTAGCTTTTTCAAAAGAACATGTCCCTCCAAAGATGGTAACTCTCTTAGCTTTGCCTCATTAGACAACTTAGCAGCTGCAGACAAGTTCTTGTCAAATTTACTAGCAAGGTCACGAACTGAGATACGCTTGTTCTGGTCTGACAAATTGGAAGCTTCTTCTTCAATAACATCTTTCAAAGAAGGACCATGGTTATCCTCAGCAGGCTCTTGAAAAACTTGATGATCGGCCCCTAGTTTATACTTCGGAAAACTATTAGAAATAAAGCTCACATCGGCCGAAACAGGCGGAACTGCTTCCATTGGCATCAAAGTTTCAATTTCAGGACTAATCTTTGTCATAGCGAAGACTAAGGCGTATAGAGCCAACCAAACAAAGCCTATGCTAGTTTCCAATTAAGCTAAATTGATTTAGGTTTGGTTTGGTTGACAAAGGTAAAAGTTAAAGTTGAAAGTAA
TARGET=303,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1556
PRIMER_LEFT_PENALTY=0.115769
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=ATGCAACCCAATTAGTGGAAAC
PRIMER_RIGHT_SEQUENCE=AGAAAAGGGCTGTCATTTGTGT
PRIMER_LEFT=88,22
PRIMER_RIGHT=351,22
PRIMER_LEFT_TM=60.116
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.2258
PRIMER_LEFT_1_PENALTY=0.123706
PRIMER_RIGHT_1_PENALTY=0.102129
PRIMER_LEFT_1_SEQUENCE=GCTACACTAGATCATCTGGCCC
PRIMER_RIGHT_1_SEQUENCE=CTATGCGGAGTCAGAAGTGATG
PRIMER_LEFT_1=225,22
PRIMER_RIGHT_1=408,22
PRIMER_LEFT_1_TM=60.124
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=12.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.2635
PRIMER_LEFT_2_PENALTY=0.123706
PRIMER_RIGHT_2_PENALTY=0.139753
PRIMER_LEFT_2_SEQUENCE=GCTACACTAGATCATCTGGCCC
PRIMER_RIGHT_2_SEQUENCE=TCAGAAGTGATGTCGGTAATGC
PRIMER_LEFT_2=225,22
PRIMER_RIGHT_2=398,22
PRIMER_LEFT_2_TM=60.124
PRIMER_RIGHT_2_TM=60.140
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=12.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.2824
PRIMER_LEFT_3_PENALTY=0.123706
PRIMER_RIGHT_3_PENALTY=0.158697
PRIMER_LEFT_3_SEQUENCE=GCTACACTAGATCATCTGGCCC
PRIMER_RIGHT_3_SEQUENCE=GAGATTTACTCCCCAACAATGC
PRIMER_LEFT_3=225,22
PRIMER_RIGHT_3=432,22
PRIMER_LEFT_3_TM=60.124
PRIMER_RIGHT_3_TM=59.841
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=12.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=0.3981
PRIMER_LEFT_4_PENALTY=0.115769
PRIMER_RIGHT_4_PENALTY=0.282356
PRIMER_LEFT_4_SEQUENCE=ATGCAACCCAATTAGTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=GGGCTGTCATTTGTGTTAACCT
PRIMER_LEFT_4=88,22
PRIMER_RIGHT_4=345,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=60.282
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_05974 Ps_contig_mira-and-tgicl-ass_franssen_40863
SEQUENCE=GGAGAAACCAAAGTAAACAAGTTCTTCTCAGAGCACCATTATTTACTCATTATTACAAGAAATGGGAGTTAGTGGAACTTTTGACTACTTGTCTGATCTAATGAGCAGTGGTCACCAGCATCTCAAGAAGAAGAAGAAGCAGTTACAAACTGTAGAGCTTAAGGTTAGAATGGACTGTGATGGCTGTGAGCTTAAGGTTAAGAAAACCCTTTCTTCTCTAAGTGGGGTAAAATCGGTAGAGATAAACCGTAAACAGCAAAAAGTAACTGTGACTGGGTTTGTGGAGCCAAACAAGGTGCTAAAGAAGGCTAAGTCAACGGGGAAGAAGGCTGAAATATGGCCTTATGTACCTTACAACTTGGTGGCTCAACCATATGCTGTTTCTTCTTATGACAAGAGGGCTCCTCCTGGTTATGTGAGAAGAGTGGAGAATGCTCCAACCACTGGAACCATGACAAGATATGAAGATCCTTATGTCAACATGTTCAGTGATGAAAACCCAAATGCATGTTCTATCATGTAAAACTAAAA
TARGET=125,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8680
PRIMER_LEFT_PENALTY=0.863548
PRIMER_RIGHT_PENALTY=0.004430
PRIMER_LEFT_SEQUENCE=ACAAGTTCTTCTCAGAGCACCA
PRIMER_RIGHT_SEQUENCE=ACCCCACTTAGAGAAGAAAGGG
PRIMER_LEFT=16,22
PRIMER_RIGHT=227,22
PRIMER_LEFT_TM=59.136
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.8680
PRIMER_LEFT_1_PENALTY=0.863548
PRIMER_RIGHT_1_PENALTY=0.004430
PRIMER_LEFT_1_SEQUENCE=ACAAGTTCTTCTCAGAGCACCA
PRIMER_RIGHT_1_SEQUENCE=CCCCACTTAGAGAAGAAAGGGT
PRIMER_LEFT_1=16,22
PRIMER_RIGHT_1=226,22
PRIMER_LEFT_1_TM=59.136
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.9210
PRIMER_LEFT_2_PENALTY=0.863548
PRIMER_RIGHT_2_PENALTY=0.057411
PRIMER_LEFT_2_SEQUENCE=ACAAGTTCTTCTCAGAGCACCA
PRIMER_RIGHT_2_SEQUENCE=TTAAGCTCACAGCCATCACAGT
PRIMER_LEFT_2=16,22
PRIMER_RIGHT_2=194,22
PRIMER_LEFT_2_TM=59.136
PRIMER_RIGHT_2_TM=59.943
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.9375
PRIMER_LEFT_3_PENALTY=0.863548
PRIMER_RIGHT_3_PENALTY=0.073989
PRIMER_LEFT_3_SEQUENCE=ACAAGTTCTTCTCAGAGCACCA
PRIMER_RIGHT_3_SEQUENCE=CTTAAGCTCACAGCCATCACAG
PRIMER_LEFT_3=16,22
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=59.136
PRIMER_RIGHT_3_TM=60.074
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.9958
PRIMER_LEFT_4_PENALTY=0.863548
PRIMER_RIGHT_4_PENALTY=0.132262
PRIMER_LEFT_4_SEQUENCE=ACAAGTTCTTCTCAGAGCACCA
PRIMER_RIGHT_4_SEQUENCE=CAGCCATCACAGTCCATTCTAA
PRIMER_LEFT_4=16,22
PRIMER_RIGHT_4=185,22
PRIMER_LEFT_4_TM=59.136
PRIMER_RIGHT_4_TM=60.132
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_05975 Ps_contig_mira-and-tgicl-ass_franssen_40868
SEQUENCE=TCGGGGACAAACAAACAGAGAACAGAGAAACAAAAACAGAGAAACGTATCCAAATTTCCATACAAACAAACAATGGCACCCGCGAGAACTCTCAGCCATCTTTAACCCAAAGAAAAAACCCTCCAGTCCAGTTTCGTTCGTGACGAAAGCGAGCGTCCAAAAGTCTCTTACAATGACTTCAGCAATGACATTCCAATCATTTCTCTCGCCGGAATCGACGATGTCCATGGCAAGGAANAACAGTTAA
TARGET=62,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3739
PRIMER_LEFT_PENALTY=1.256069
PRIMER_RIGHT_PENALTY=0.117817
PRIMER_LEFT_SEQUENCE=GGGACAAACAAACAGAGAACAG
PRIMER_RIGHT_SEQUENCE=TTGGAATGTCATTGCTGAAGTC
PRIMER_LEFT=3,22
PRIMER_RIGHT=195,22
PRIMER_LEFT_TM=58.744
PRIMER_RIGHT_TM=60.118
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=1.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=193
PRIMER_PAIR_PENALTY_1=1.3783
PRIMER_LEFT_1_PENALTY=1.256069
PRIMER_RIGHT_1_PENALTY=0.122257
PRIMER_LEFT_1_SEQUENCE=GGGACAAACAAACAGAGAACAG
PRIMER_RIGHT_1_SEQUENCE=CTTTGGGTTAAAGATGGCTGAG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=111,22
PRIMER_LEFT_1_TM=58.744
PRIMER_RIGHT_1_TM=60.122
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=1.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=109
PRIMER_PAIR_PENALTY_2=1.3864
PRIMER_LEFT_2_PENALTY=1.256069
PRIMER_RIGHT_2_PENALTY=0.130358
PRIMER_LEFT_2_SEQUENCE=GGGACAAACAAACAGAGAACAG
PRIMER_RIGHT_2_SEQUENCE=TGGGTTAAAGATGGCTGAGAGT
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=108,22
PRIMER_LEFT_2_TM=58.744
PRIMER_RIGHT_2_TM=60.130
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=1.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=106
PRIMER_PAIR_PENALTY_3=1.4187
PRIMER_LEFT_3_PENALTY=1.300876
PRIMER_RIGHT_3_PENALTY=0.117817
PRIMER_LEFT_3_SEQUENCE=GGGGACAAACAAACAGAGAACA
PRIMER_RIGHT_3_SEQUENCE=TTGGAATGTCATTGCTGAAGTC
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=61.301
PRIMER_RIGHT_3_TM=60.118
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=1.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=194
PRIMER_PAIR_PENALTY_4=1.4231
PRIMER_LEFT_4_PENALTY=1.300876
PRIMER_RIGHT_4_PENALTY=0.122257
PRIMER_LEFT_4_SEQUENCE=GGGGACAAACAAACAGAGAACA
PRIMER_RIGHT_4_SEQUENCE=CTTTGGGTTAAAGATGGCTGAG
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=111,22
PRIMER_LEFT_4_TM=61.301
PRIMER_RIGHT_4_TM=60.122
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=1.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=110
=
PRIMER_SEQUENCE_ID=SSR_05976 Ps_contig_mira-and-tgicl-ass_franssen_40870
SEQUENCE=AACTGATTATGAGAAGAGAAAACTTGTTGCTCTTGCACTTGAGGATACGGCAATGGAACAAAGAAAGACGGATTATCAGAGAATTGAACTTGGTTATGATTCATCATAAAATTAGGGTTAATAACTTGAGGCTTCTCGTCTACAACCCCAAACCCTTGTTGTTGCTGTTGTTGCTGAGAACAAAACACACCAGAAGTTGTCGACGACGATGTCAACATAGGATAGTTACCAAACATAGATTCAGTACCAGGTTTCACATTGTGACTATCCAAATTCTCAGCATTGAAAGGAAAAACATTGTAATTCCCAACAGAAGGGTCAATGTTCAAACCTTGTTGTTGTTGATGATGTTCCAAAACACCGAAACCGCCATGAAAATCGGCATTTTCAGAACCACAACCACTGTTTTGCAAGATTTTG
TARGET=333,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4700
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.461740
PRIMER_LEFT_SEQUENCE=CAACAGAAGGGTCAATGTTCAA
PRIMER_RIGHT_SEQUENCE=AACAGTGGTTGTGGTTCTGAAA
PRIMER_LEFT=307,22
PRIMER_RIGHT=406,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.538
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=100
PRIMER_PAIR_PENALTY_1=0.4700
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.461740
PRIMER_LEFT_1_SEQUENCE=CAACAGAAGGGTCAATGTTCAA
PRIMER_RIGHT_1_SEQUENCE=AAAACAGTGGTTGTGGTTCTGA
PRIMER_LEFT_1=307,22
PRIMER_RIGHT_1=408,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=59.538
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=102
PRIMER_PAIR_PENALTY_2=0.4860
PRIMER_LEFT_2_PENALTY=0.008257
PRIMER_RIGHT_2_PENALTY=0.477719
PRIMER_LEFT_2_SEQUENCE=CAACAGAAGGGTCAATGTTCAA
PRIMER_RIGHT_2_SEQUENCE=CAAAACAGTGGTTGTGGTTCTG
PRIMER_LEFT_2=307,22
PRIMER_RIGHT_2=409,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.478
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=103
PRIMER_PAIR_PENALTY_3=0.6827
PRIMER_LEFT_3_PENALTY=0.220992
PRIMER_RIGHT_3_PENALTY=0.461740
PRIMER_LEFT_3_SEQUENCE=GTAATTCCCAACAGAAGGGTCA
PRIMER_RIGHT_3_SEQUENCE=ACAGTGGTTGTGGTTCTGAAAA
PRIMER_LEFT_3=299,22
PRIMER_RIGHT_3=405,22
PRIMER_LEFT_3_TM=60.221
PRIMER_RIGHT_3_TM=59.538
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=0.6827
PRIMER_LEFT_4_PENALTY=0.220992
PRIMER_RIGHT_4_PENALTY=0.461740
PRIMER_LEFT_4_SEQUENCE=TGTAATTCCCAACAGAAGGGTC
PRIMER_RIGHT_4_SEQUENCE=ACAGTGGTTGTGGTTCTGAAAA
PRIMER_LEFT_4=298,22
PRIMER_RIGHT_4=405,22
PRIMER_LEFT_4_TM=60.221
PRIMER_RIGHT_4_TM=59.538
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=108
=
PRIMER_SEQUENCE_ID=SSR_05977 Ps_contig_mira-and-tgicl-ass_franssen_40872
SEQUENCE=TGTGAATAAGTATTGTTACTACAAAAGAGATAAGACTCGTGGAGCACTATCTCTTAACTCCATTCACAAATTTCCTTGCCAACAAAAGCACTATTACATACATATCTAAAGCCTAACCTCATATCATAAAATTCCTTGTCAATGTCAGTTCATAAGAATCAAAACATCATCAAGTTTGTGGCAGTCCCAACAATAAACGGTTCCATTAGCAATCTAGTCAAGAAATAGAAAAGCACACCCAATGCAATCGAAATGGGGAGCGCAGGCAGAAGCATGACGACAGACAGACAACAAAATAAGAGTGCAGCCAAGTCCAGATATAATTGCAAGATAACAAGCATAGACAGTCATGAGATCATACATTGCAGCTCTACCCACAAGAACACTGTAGAAAACAAAGTCACCAAGTCCAAGCTTAATACCTCTCTCAGAAACTTCTTCTGCTTCTCTCGTCGCCATTTCTCTCTCAGTCCTATTCACCTCAACAAGTGGAGACCTTTCCTCCTCACGATCAACATTACCAACCAATGGAGATCTTTCCCCTTCGTCGTTACGAAGATCCTCAACCCTAACAACAGCAACAGAATCACCAGCACTATGAGTGTTCCTATTACCCTCATTCCCAGTAGGGACCACCTGAAGCTCAATTGACGACGATCCCAATCCCGATTCGGTAGCACCAGCTACCAAAAACCCTAATGTAGCAGCCCTACCGGTCGGGGCAACAGTAGGGCGAGCTTCATAGATTAAAGCAGGAAGCTCCTCGTTCCGGCTAGAGGCCAAATCAACAAGAAGCTTCAACGGACCACCGGGAGCCAAAACAGCGACAAGATCGTAAACAGCGAGAGCCACAAGTAAAGTCCAAGTCGTCCATTCAGGAAGCTTGGTAAACCACGCGGCAACGATGATACCGAGG
TARGET=279,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2467
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.209343
PRIMER_LEFT_SEQUENCE=AACATCATCAAGTTTGTGGCAG
PRIMER_RIGHT_SEQUENCE=AGCTTGGACTTGGTGACTTTGT
PRIMER_LEFT=162,22
PRIMER_RIGHT=415,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=60.209
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.2482
PRIMER_LEFT_1_PENALTY=0.037339
PRIMER_RIGHT_1_PENALTY=0.210886
PRIMER_LEFT_1_SEQUENCE=AACATCATCAAGTTTGTGGCAG
PRIMER_RIGHT_1_SEQUENCE=GGACTTGGCTGCACTCTTATTT
PRIMER_LEFT_1=162,22
PRIMER_RIGHT_1=314,22
PRIMER_LEFT_1_TM=60.037
PRIMER_RIGHT_1_TM=59.789
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=153
PRIMER_PAIR_PENALTY_2=0.3600
PRIMER_LEFT_2_PENALTY=0.037339
PRIMER_RIGHT_2_PENALTY=0.322707
PRIMER_LEFT_2_SEQUENCE=AACATCATCAAGTTTGTGGCAG
PRIMER_RIGHT_2_SEQUENCE=CGACGAGAGAAGCAGAAGAAGT
PRIMER_LEFT_2=162,22
PRIMER_RIGHT_2=454,22
PRIMER_LEFT_2_TM=60.037
PRIMER_RIGHT_2_TM=60.323
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=0.3776
PRIMER_LEFT_3_PENALTY=0.037339
PRIMER_RIGHT_3_PENALTY=0.340219
PRIMER_LEFT_3_SEQUENCE=AACATCATCAAGTTTGTGGCAG
PRIMER_RIGHT_3_SEQUENCE=GTGGGTAGAGCTGCAATGTATG
PRIMER_LEFT_3=162,22
PRIMER_RIGHT_3=377,22
PRIMER_LEFT_3_TM=60.037
PRIMER_RIGHT_3_TM=59.660
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=216
PRIMER_PAIR_PENALTY_4=0.3776
PRIMER_LEFT_4_PENALTY=0.037339
PRIMER_RIGHT_4_PENALTY=0.340219
PRIMER_LEFT_4_SEQUENCE=AACATCATCAAGTTTGTGGCAG
PRIMER_RIGHT_4_SEQUENCE=TGTGGGTAGAGCTGCAATGTAT
PRIMER_LEFT_4=162,22
PRIMER_RIGHT_4=378,22
PRIMER_LEFT_4_TM=60.037
PRIMER_RIGHT_4_TM=59.660
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_05978 Ps_contig_mira-and-tgicl-ass_franssen_40878
SEQUENCE=GAAGTTTTCAATGGGAGTTAATAGGTTACAGCAGATGAAGAGTCATAGTGGAAATAATTCTAATCTTATTAGACATAGTAGTTCACCAGCTGGATTATTTTCTCAAATCAACATTGAAAATGGGTATGTTAGTATGAGAGGAATGGGAAATTTAGGAGCGGTTAATAGTTCTATGAAAGATGCAAAATTTTCTTCTGGAAGGAGTTTGAAGAATTCAGCACACTATTCATCAGGGGTAATGTCAACAATAGCTGAAGTTGGGGACAAATGCAATGAAGAAAATAATTTAGAAAGTGAAGTTTTTGGTGAAAGTCACGGTAACGATTATATCGTGGATTATCAGGTTGTTGATACTTGGGATGATACCGAAATGATGTCTGAAAATGTTGGTGGTTTGAAAAGATTTAGAGATAGTGATTCAAAACAACAATTTTCTGCAGGTTTTAATGCAGCTGCAGTTCAGAATGAAACAGGAGGAGGGCATTCATCTTCTCCATTGGCTCATCAATTGAGTATGCCTAATACTTCATCTGAAATGGCAGCTATTGAGAAGTTTCTGCATTTCTCAGATTCTGTTCCGATGAAAATTCGCGCTAAGCGTGGTTGTGCTACTCACCCGAGAAGCATAGCAGAAAGGGTTAGAAGGACTAAAATTAGTGAACGCATGAGGAAGCTACAAGATCTCGTCCCAAACATGGATAAGCAAACAAATACAGCAGATATGTTAGACTTGGCTGTGGATTACATCAAAGATCTTCAAAAGCAAGCTCAGAAGCTTCAAGATTGTCAAGCAAAGTGCACTTGCTCACACAAGAAGCAACAATAACATCAAGAATGAATGAATTTCTTAGTCCATGTATAGATTTGTAATAATTTAATTTGGTCACCAAGTTAAAA
TARGET=833,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05979 Ps_contig_mira-and-tgicl-ass_franssen_40883
SEQUENCE=AATTTTTGGAAACTCGAAAAAATCTTTTAGGATACTAGGTGATGATTTTTGGTTCTTTCCATCCCGAAAAATCCACATCCTTGTTATAGTAACAGCACAATCCACACCGGTCACTAAGAATGGTTGCATGCTCCCAAAGTAAAAAAATTCAAAAGAGATGACCATTCGTTACATGTTTAGATATCTTACAAACCAAGTAATATATAGTAGATTAGCGTAATCGGTAGCGCTATCAGCATTCCGAATATAACCCCCGTACTAAGAATGGCCGGATGAACATTGTACTCTTTCGCAAACACAAATGGAACAATCCCTTGAGGAAGTGCCGCCTGAACAATTAGTCTTACGCGTAAAAGGTTACCACGCAGGCCAACCGCAATGGAAGCCGCTGCCATGACTGCGGGACCGGTAAGAAACCGAACCGCCACAGCAAATGAAGCAACCGAGTTTCCACATGCAATCATCTTAGGCTGTAGAGCCATAAACAAACCTAACTGAACATAGCCATTCCAAGACCAGCATCAGACAGTATGGAGATTGATTTCTCTATTATTTTGGGCATATGCACATGCCACCTAAAGGCCACTAGGGACCAAATGACACCAATGAGGCTGGAATATGTGTTTGGGTTGCGGATTAGCTTCCGCCAAACCATGATCAGTATGAGACGGGTCATAACGCTTGGCCGGGGGCATGTGTTTTGCGGTGGTGGCAACGGTGTTTGTCGGGTGTAGCTCTGCCGTTGAGCTTGATCCTAGTCTTTGTTCACGGCCCCGGGGTCGGGGGTTCCTTTCTTTCTCGTCGGCGATTCTTTCTTCTAGAGTTGGTAGTTTTCCTTTCTTTCTTTCACGTTAGGTAAAATTTTCCAAACCTTTCCTTTTCCCTACCCCAAA
TARGET=836,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2976
PRIMER_LEFT_PENALTY=0.093026
PRIMER_RIGHT_PENALTY=0.204533
PRIMER_LEFT_SEQUENCE=CCGTTGAGCTTGATCCTAGTCT
PRIMER_RIGHT_SEQUENCE=GTAGGGAAAAGGAAAGGTTTGG
PRIMER_LEFT=739,22
PRIMER_RIGHT=886,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.205
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=8.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=0.2976
PRIMER_LEFT_1_PENALTY=0.093026
PRIMER_RIGHT_1_PENALTY=0.204533
PRIMER_LEFT_1_SEQUENCE=CCGTTGAGCTTGATCCTAGTCT
PRIMER_RIGHT_1_SEQUENCE=GGTAGGGAAAAGGAAAGGTTTG
PRIMER_LEFT_1=739,22
PRIMER_RIGHT_1=887,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=60.205
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.3420
PRIMER_LEFT_2_PENALTY=0.137489
PRIMER_RIGHT_2_PENALTY=0.204533
PRIMER_LEFT_2_SEQUENCE=GATCCTAGTCTTTGTTCACGGC
PRIMER_RIGHT_2_SEQUENCE=GTAGGGAAAAGGAAAGGTTTGG
PRIMER_LEFT_2=750,22
PRIMER_RIGHT_2=886,22
PRIMER_LEFT_2_TM=60.137
PRIMER_RIGHT_2_TM=60.205
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=137
PRIMER_PAIR_PENALTY_3=0.3420
PRIMER_LEFT_3_PENALTY=0.137489
PRIMER_RIGHT_3_PENALTY=0.204533
PRIMER_LEFT_3_SEQUENCE=GATCCTAGTCTTTGTTCACGGC
PRIMER_RIGHT_3_SEQUENCE=GGTAGGGAAAAGGAAAGGTTTG
PRIMER_LEFT_3=750,22
PRIMER_RIGHT_3=887,22
PRIMER_LEFT_3_TM=60.137
PRIMER_RIGHT_3_TM=60.205
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=11.1000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=0.6052
PRIMER_LEFT_4_PENALTY=0.400659
PRIMER_RIGHT_4_PENALTY=0.204533
PRIMER_LEFT_4_SEQUENCE=GTATGAGACGGGTCATAACGCT
PRIMER_RIGHT_4_SEQUENCE=GTAGGGAAAAGGAAAGGTTTGG
PRIMER_LEFT_4=660,22
PRIMER_RIGHT_4=886,22
PRIMER_LEFT_4_TM=60.401
PRIMER_RIGHT_4_TM=60.205
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_05980 Ps_contig_mira-and-tgicl-ass_franssen_40890
SEQUENCE=CGGGGGAGATANNTATATAGGATTTATTAACACAATCTTCTTCTTCTTTCTTCTTCCTGTAAAAAAAACACAATCTTCTTCTTCTGGTTCGATTTTGGAACAACTCAGAAGCAGAACTCGACGCAACTCTCTTCGCCATGTGTAGTCTCATTTCAAAGTCACACTGTCACTTAGGATTGGGGAAACTCTCTTTGCCACCTCTTCAAACAAGTAAGTCAGAATATGGAATTGTGAGAGTGAAAATGAGTGGGAGATTGAAGGTGGTGTGTAAGGGTATGTTGACACCGAGAAAATTTATGCAGAGGAAAAAGCAAACGGTGGTTTTCAAGGATGATGCCGATGAAGCTAAGAAGAAAAATTGGTGGAGATTAATGAAATTAATAGAGGAGTCTGGCTCTGCTGTTTCTGTGCTCTCTTCTGAGAAGATGAAGAACCAAACTATTCCTAAAGACCTTGTTGTTGGTACTTTGATTAGGTTTAAACAACTCAAGAAGTGGAATGTTGTTGCTGAGATTCTTGAATGGCTTAGAGCACAAAACTGGTGGGATTTTAGTAAGATGGATTTCATCATGCTCATTACGGTCTTATGGGTAAAGGGTTRGG
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05981 Ps_contig_mira-and-tgicl-ass_franssen_40890
SEQUENCE=CGGGGGAGATANNTATATAGGATTTATTAACACAATCTTCTTCTTCTTTCTTCTTCCTGTAAAAAAAACACAATCTTCTTCTTCTGGTTCGATTTTGGAACAACTCAGAAGCAGAACTCGACGCAACTCTCTTCGCCATGTGTAGTCTCATTTCAAAGTCACACTGTCACTTAGGATTGGGGAAACTCTCTTTGCCACCTCTTCAAACAAGTAAGTCAGAATATGGAATTGTGAGAGTGAAAATGAGTGGGAGATTGAAGGTGGTGTGTAAGGGTATGTTGACACCGAGAAAATTTATGCAGAGGAAAAAGCAAACGGTGGTTTTCAAGGATGATGCCGATGAAGCTAAGAAGAAAAATTGGTGGAGATTAATGAAATTAATAGAGGAGTCTGGCTCTGCTGTTTCTGTGCTCTCTTCTGAGAAGATGAAGAACCAAACTATTCCTAAAGACCTTGTTGTTGGTACTTTGATTAGGTTTAAACAACTCAAGAAGTGGAATGTTGTTGCTGAGATTCTTGAATGGCTTAGAGCACAAAACTGGTGGGATTTTAGTAAGATGGATTTCATCATGCTCATTACGGTCTTATGGGTAAAGGGTTRGG
TARGET=74,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05982 Ps_contig_mira-and-tgicl-ass_franssen_40909
SEQUENCE=AAAGAAACAATGCCCAATTAATTATATACCATTTTGTTTCACTTTCAACATTATTATTATTATTATTATATATCTTCCTNGATTTTTYYTTTTTCCAATTTCAGAAACTTAATTCAAATCACTCATTCACACAGCAATTACATGTTCACTTCAAACTTTCCACTTTTGCTATGAAACCAATTTTTGTAGCTAACTAGAAATTGAAAGTGAAATTGGAATGGGAATTTTAAGTACCATAACTGGTTTCTTGGGATTTGGAATTGGAACTTCAATTGGATTATTCATTGGTTATTATATTTTTATATACTTTCAATCAATACATGTTAAGGAACCTACATTTAGTCCTTTGGTTTGAACAAGATTTGAAAACTGTGCAACAATTGCTTCCAGAGATACCTCTTTGGATTAAAAATCCAGATTATGATCGTCTTGATTGGCTTAATAAATTTGTTGAGTATATGTGGCCTTATTTGAATAAGGCAATTTGTAAGACTGCAAGAACTATAGCAAAGCCAATTATTGCTGAACAAATTCCAAAGTATAAAATTGATTCTGTTGAATTTGAGGAACTTAACTTGGGTTCTTTACCACCAACTTTTCAAGGAATGAAAGTTTATAGTACTGATGAAAAGGAATTGATTATGGAATTATCAATGAAATGGGCTGGGAATCCTAACATTATAGTTGCAGTTAAAGCATTTGGATTGCGAGCCACAGTTCAGGTTGTTGATCTTCAAGTATTTGCTTCTCCGCGCATAACACTGAGGCCTTTGGTTCCTTAGTTTTTCCTTGTTTCNYCRTTTCCTTTTAATTTTTATTTT
TARGET=50,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05983 Ps_contig_mira-and-tgicl-ass_franssen_40911
SEQUENCE=ACATAAAATACTCTATACACTTTCAACACCAAAGTAGTATCTATAACATCCCCCTTAAACTTCTTCCAATTTCTCAATGATGAATCAACATCTTATCTTATTNTTAACACTTGCAAAGATCCTAGCAATCTCAAGTTGTGTTATTAGCGANTAATTTCTAGTTCCTTCTTGATTTCCATTTCCCCTCTCTTTGCCTCAGCTTCAACTCTCATCTTCTCTATCTCTTTCAATGTGTCCATTCTTGTTTGCTCTGACCTTACAACAACTTCTGCTAGCCACCTTATGCTTTCTGCTATTCCTATTGATTCTTTCTTCTGTCTCTTGTTATTGTTGTTCTCCGATCTCATTCTCCGCTTTTTGCATCTTTGTTCGTCTTTTTCGCTGTAGAGCGCAGGCGTGCTGCTATCGGTTTCCAATGGATTCTTGTTAGGTCCATGATCAGATGACTTAGTTTCTAACTCTTCTTCCTTGGCTAATTTATCAAACCCATTTGATCCATGAGAGTTTTGAGTAGTCGCAATTAATGGTGGATTAGCAACACTAGTAGGATCTGGTAGCTGATTAGCTTCATCAATCCCTTGTTGTGAAGGTTCCAACAAAACCAAACCATGATTAATATTACTATTATTATTATTATTACTACTTACTTGTAGCAATTTCGTAGCAACATTCGTAGCTAATTGTCCCGTCCCACGAAGAAGAAGATCAAGCCTAGAATACAAAGGCCAAGCAGAAACATCTGAGCTAGCTGACTCCGATCTATACCTTTTCTTCATTGACTCAATCTTATTCTTACACTGAGTTTGTGTTTTCGGCGACTTAGTCGAATTCGATCGCGACGAAACATGTCTCGCGACGTCCTCCCAATCTTGTCCTTTGAGTTTTGCTCGGTTTCTGAGTATCCATTTTGATTCATAGGACTCAAGAAGTGTTGTCACTGCTCCTTCACTCCATTCATCTCTTTTTAGTCTCTCATTTGTTGAGTTGATGATGATGGTTTGTTGTTTCTTTTGTGAGTCTTGTGATGTTGGAGGGTTTTGGTCTTTGGTTTCTTGTTTTTCATTCATTGCTTTTGAACATGATAATAGGATCCATCTAGTGTGAGTTTTTTGAGAGTTTGTAGTGTTTTGATGATAACATTTTGTTGCAAAGTTAGAAGGTCACNTGAGGTTGAAGAATATGAAAGGGTCCCCGAGTACT
TARGET=623,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0232
PRIMER_LEFT_PENALTY=0.004359
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=AGCTTCATCAATCCCTTGTTGT
PRIMER_RIGHT_SEQUENCE=AGGCTTGATCTTCTTCTTCGTG
PRIMER_LEFT=563,22
PRIMER_RIGHT=712,22
PRIMER_LEFT_TM=60.004
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=150
PRIMER_PAIR_PENALTY_1=0.0481
PRIMER_LEFT_1_PENALTY=0.029253
PRIMER_RIGHT_1_PENALTY=0.018835
PRIMER_LEFT_1_SEQUENCE=TCATCAATCCCTTGTTGTGAAG
PRIMER_RIGHT_1_SEQUENCE=AGGCTTGATCTTCTTCTTCGTG
PRIMER_LEFT_1=567,22
PRIMER_RIGHT_1=712,22
PRIMER_LEFT_1_TM=59.971
PRIMER_RIGHT_1_TM=60.019
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=0.0481
PRIMER_LEFT_2_PENALTY=0.029253
PRIMER_RIGHT_2_PENALTY=0.018835
PRIMER_LEFT_2_SEQUENCE=CTTCATCAATCCCTTGTTGTGA
PRIMER_RIGHT_2_SEQUENCE=AGGCTTGATCTTCTTCTTCGTG
PRIMER_LEFT_2=565,22
PRIMER_RIGHT_2=712,22
PRIMER_LEFT_2_TM=59.971
PRIMER_RIGHT_2_TM=60.019
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=148
PRIMER_PAIR_PENALTY_3=0.0868
PRIMER_LEFT_3_PENALTY=0.067994
PRIMER_RIGHT_3_PENALTY=0.018835
PRIMER_LEFT_3_SEQUENCE=GCTGATTAGCTTCATCAATCCC
PRIMER_RIGHT_3_SEQUENCE=AGGCTTGATCTTCTTCTTCGTG
PRIMER_LEFT_3=556,22
PRIMER_RIGHT_3=712,22
PRIMER_LEFT_3_TM=60.068
PRIMER_RIGHT_3_TM=60.019
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.3000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=157
PRIMER_PAIR_PENALTY_4=0.2058
PRIMER_LEFT_4_PENALTY=0.186949
PRIMER_RIGHT_4_PENALTY=0.018835
PRIMER_LEFT_4_SEQUENCE=CCCATTTGATCCATGAGAGTTT
PRIMER_RIGHT_4_SEQUENCE=AGGCTTGATCTTCTTCTTCGTG
PRIMER_LEFT_4=485,22
PRIMER_RIGHT_4=712,22
PRIMER_LEFT_4_TM=60.187
PRIMER_RIGHT_4_TM=60.019
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_05984 Ps_contig_mira-and-tgicl-ass_franssen_40916
SEQUENCE=AACGCAGAGTACGCGGGGAATGAAAAATAAGAGCACCAAAATGTTGACTTCAAATTAATAATACAACTCCTACTCCATTAAACAAAAACATCCTTCTATGGCATCTCTCATTATTGGACCTTGTTGGTGTAAACTAGGGACCCCTTTTGTTCCATTGTCTTGCCAAGGTAAAAGACCATCCATCTTTAATTCCAATCATGAAGAAAAGAAAGACATAGGAAGTGGAGGAAAGGGAAGCAACCATTATGGAAACGGAAAGTAATTTTGAATGATTTTGAGAAGGTAATTGGGAAGAGGCTACAGAAGATGAAGAATTTAAGTCCACAACAACAAAAGGGTGATTGGAAAGATTTGTTTCTAATGAGTATTTCTTTTTCTGTTTATGTGTATATATCTCAGAAGCTAGTTTGTGCTTATTGTGCTTGGACTTCATTGCCTAAAGTAAACGTGTGGTAGTTAGTTATTCATTTCTTTACTTTTGTTGAAAGCTATAAATTGTAAGCACTAGATTTTTTTTAGAAGGGGAATATGATTGTGTATTACATGTGTAAAAGATAAGATAAGATGTATTTTGAAGTTAGCAAAATCAATAATATTTAAGTAGTTAACTTCAAG
TARGET=552,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05985 Ps_contig_mira-and-tgicl-ass_franssen_40923
SEQUENCE=TCCTTTCTCTCTCTCTTTTTCTATATCCCTTAAACACACTGTTAGAACAAAAACAACACCGCCAAAAAAAAATGAGTACCTCTTTCTTTGAATTCAAGAAACAAGCTTCTTTCTTCCTCAAAGAGAAAATCAAAACCGCTAGATTAGCTTTAACAGATGTCACTCCTGCACAACTCATGGTAGAAGAGGCAACCAATGGAAATCCATGGGCTCCAACTACTGTAACGCTCAG
TARGET=6,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05986 Ps_contig_mira-and-tgicl-ass_franssen_40929
SEQUENCE=CTTAGACAATTACCAATACTGTTATGACATTGTTTGGTTTATTTGGTGGGTCTCATTATGACCTGGTATGAGGAAAAACCAATTACAATGAAATGGATAAGGTTGTTGTTGTTTACCAAATATTGGCTGTAGAATGATATTGTACTAATATAGTACTAATAGTCATTTTTAAACCTTAAGAAGGATATGTGATGATTATGTAATCCTACTTTATTTCTACGTCAAATTTAAA
TARGET=102,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05987 Ps_contig_mira-and-tgicl-ass_franssen_40935
SEQUENCE=AGAAATAGAGTAAAAAAGAAGGGAAAGANANGTNGGNNNTAGCAANAGAGTAGCAATTGAATTGAGAGAGCAAAAGGCTTTCTAACTCAACTGGTGTGTCACTCGCTGACCCCAACGGCAAAGCCCGCGCCGCTTTCCATTTCAACTTCAACTTGTTTTTCTCCACTTTCACCCCCAACATTTAGGGTTTATCACCATGAGAGCCAAATTGATCGTCTTCCCCATACGAGCCAGAAACTGGTGTTTCACCAGATCCATTGATCATTCTCTCCCTCCTCCTCCTTCTTCATCCGCTTCCTCCTTCAAAGAATTATGGAACAACATTAACAATGCTGAAAAATCCTTCAATGCCAAAGTTGAACTCTGTACCGATTACGTCGCTGACAAGATGAATAATGGCTGGGTTACTTTGGAAAAAGCACCTGATGGATCGTTGAAAAAGAAGATTCACGGGGTTGGGGGTTATGGTCTTTTGTCTC
TARGET=272,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0761
PRIMER_LEFT_PENALTY=0.056343
PRIMER_RIGHT_PENALTY=0.019805
PRIMER_LEFT_SEQUENCE=CCCCAACATTTAGGGTTTATCA
PRIMER_RIGHT_SEQUENCE=CCCGTGAATCTTCTTTTTCAAC
PRIMER_LEFT=172,22
PRIMER_RIGHT=453,22
PRIMER_LEFT_TM=59.944
PRIMER_RIGHT_TM=59.980
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=282
PRIMER_PAIR_PENALTY_1=0.0806
PRIMER_LEFT_1_PENALTY=0.060824
PRIMER_RIGHT_1_PENALTY=0.019805
PRIMER_LEFT_1_SEQUENCE=ATACGAGCCAGAAACTGGTGTT
PRIMER_RIGHT_1_SEQUENCE=CCCGTGAATCTTCTTTTTCAAC
PRIMER_LEFT_1=223,22
PRIMER_RIGHT_1=453,22
PRIMER_LEFT_1_TM=60.061
PRIMER_RIGHT_1_TM=59.980
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.0965
PRIMER_LEFT_2_PENALTY=0.056343
PRIMER_RIGHT_2_PENALTY=0.040146
PRIMER_LEFT_2_SEQUENCE=CCCCAACATTTAGGGTTTATCA
PRIMER_RIGHT_2_SEQUENCE=CCAGCCATTATTCATCTTGTCA
PRIMER_LEFT_2=172,22
PRIMER_RIGHT_2=402,22
PRIMER_LEFT_2_TM=59.944
PRIMER_RIGHT_2_TM=59.960
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.1010
PRIMER_LEFT_3_PENALTY=0.060824
PRIMER_RIGHT_3_PENALTY=0.040146
PRIMER_LEFT_3_SEQUENCE=ATACGAGCCAGAAACTGGTGTT
PRIMER_RIGHT_3_SEQUENCE=CCAGCCATTATTCATCTTGTCA
PRIMER_LEFT_3=223,22
PRIMER_RIGHT_3=402,22
PRIMER_LEFT_3_TM=60.061
PRIMER_RIGHT_3_TM=59.960
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=0.1224
PRIMER_LEFT_4_PENALTY=0.102638
PRIMER_RIGHT_4_PENALTY=0.019805
PRIMER_LEFT_4_SEQUENCE=ATGAGAGCCAAATTGATCGTCT
PRIMER_RIGHT_4_SEQUENCE=CCCGTGAATCTTCTTTTTCAAC
PRIMER_LEFT_4=196,22
PRIMER_RIGHT_4=453,22
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=59.980
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_05988 Ps_contig_mira-and-tgicl-ass_franssen_40937
SEQUENCE=GGAGAAAGAGAGAAGAATAGAGAGAAGAGAAGAGAAAAGGAAGAAGAAGCATATAAAGGTGTAGATTGTGAAGATGGAGGACCTTTTGGTGCTGTTATTGTTTTTAATGATGAAGCTGTTGCTAGCTCTCACAATATGGTTCTCAGAAACAATGACCCTACTGCTCATGCTGAAGTCACAGCAATAAGAGAGGCATGTAAAGAA
TARGET=21,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05989 Ps_contig_mira-and-tgicl-ass_franssen_40943
SEQUENCE=CTGTAAGGAGCGTGTGATTTATTCGCTGCCTCCAAAGCTGCAATCTTTAACTTCATATCTATAGTGTCACCGTTGCAAACGCCATTGGGTAGTTTCTGTGTTGGTAATGGAAGCAGTGTTAACCCGTTGTTACGTGGCTCCAAGAAAAGAGGAGTTTGTTGAGAGAGAAGATCGTGTGGACCGAATCTGTATGGGAGAAACCGAGACAGCGGAGTGGGTTTCGGGTCGGGTTCCGAGGTTACGATGATTTGAATGTCGGGTGCTCCGCGGAGTTCTTGGAGAAACTGTCGGCAGTGGCCACAGGGTGCGGCGGAGACGGCGAAGGACTGGAGATTTGGCTCATTGTTGAGAGAGAGATTTGTGAGGAGAAACT
TARGET=348,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05990 Ps_contig_mira-and-tgicl-ass_franssen_40948
SEQUENCE=ACGAAATACAACCGGTAAGGTAAACGTTTAGTATTAANAAAGGGGTTTTAAAGGGGTTAAGGGTACGTTTTCCTAATGAGTTTCCTATCTATGATACTATCATCAACGGCTAAAACATGGAACTGTGATTCTGTTGCAGCCACTACCTATTTAGGAAAGAAAAGGTAATAGAAATAGGAAAGGAAGTAGTTTGGGTTTTGGTTTTAGTTTAGTAGGAAGGAAGGAAGAGTAGGAAGTAAGTTAAGGAAAGTAGTAGGTAGTTACGTTTAAGGTAAAGGAAAACGTAAAAACCGTAAACCCGAAACCCCCAAAACCCTAAAACCTACCCCAACCTAACCCTAACCCAAAACGAAACGAACGAACGTACCTACCGACTA
TARGET=214,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0678
PRIMER_LEFT_PENALTY=0.066394
PRIMER_RIGHT_PENALTY=0.001455
PRIMER_LEFT_SEQUENCE=AACGGCTAAAACATGGAACTGT
PRIMER_RIGHT_SEQUENCE=TTAGGGTTAGGTTGGGGTAGGT
PRIMER_LEFT=104,22
PRIMER_RIGHT=341,22
PRIMER_LEFT_TM=59.934
PRIMER_RIGHT_TM=59.999
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=238
PRIMER_PAIR_PENALTY_1=0.0678
PRIMER_LEFT_1_PENALTY=0.066394
PRIMER_RIGHT_1_PENALTY=0.001455
PRIMER_LEFT_1_SEQUENCE=AACGGCTAAAACATGGAACTGT
PRIMER_RIGHT_1_SEQUENCE=GTTAGGGTTAGGTTGGGGTAGG
PRIMER_LEFT_1=104,22
PRIMER_RIGHT_1=342,22
PRIMER_LEFT_1_TM=59.934
PRIMER_RIGHT_1_TM=59.999
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=239
PRIMER_PAIR_PENALTY_2=0.0678
PRIMER_LEFT_2_PENALTY=0.066394
PRIMER_RIGHT_2_PENALTY=0.001455
PRIMER_LEFT_2_SEQUENCE=AACGGCTAAAACATGGAACTGT
PRIMER_RIGHT_2_SEQUENCE=GGTTAGGGTTAGGTTGGGGTAG
PRIMER_LEFT_2=104,22
PRIMER_RIGHT_2=343,22
PRIMER_LEFT_2_TM=59.934
PRIMER_RIGHT_2_TM=59.999
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=0.0678
PRIMER_LEFT_3_PENALTY=0.066394
PRIMER_RIGHT_3_PENALTY=0.001455
PRIMER_LEFT_3_SEQUENCE=AACGGCTAAAACATGGAACTGT
PRIMER_RIGHT_3_SEQUENCE=TAGGGTTAGGTTGGGGTAGGTT
PRIMER_LEFT_3=104,22
PRIMER_RIGHT_3=340,22
PRIMER_LEFT_3_TM=59.934
PRIMER_RIGHT_3_TM=59.999
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=237
PRIMER_PAIR_PENALTY_4=0.0840
PRIMER_LEFT_4_PENALTY=0.066394
PRIMER_RIGHT_4_PENALTY=0.017652
PRIMER_LEFT_4_SEQUENCE=AACGGCTAAAACATGGAACTGT
PRIMER_RIGHT_4_SEQUENCE=TTGGGGTAGGTTTTAGGGTTTT
PRIMER_LEFT_4=104,22
PRIMER_RIGHT_4=330,22
PRIMER_LEFT_4_TM=59.934
PRIMER_RIGHT_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=227
=
PRIMER_SEQUENCE_ID=SSR_05991 Ps_contig_mira-and-tgicl-ass_franssen_40948
SEQUENCE=ACGAAATACAACCGGTAAGGTAAACGTTTAGTATTAANAAAGGGGTTTTAAAGGGGTTAAGGGTACGTTTTCCTAATGAGTTTCCTATCTATGATACTATCATCAACGGCTAAAACATGGAACTGTGATTCTGTTGCAGCCACTACCTATTTAGGAAAGAAAAGGTAATAGAAATAGGAAAGGAAGTAGTTTGGGTTTTGGTTTTAGTTTAGTAGGAAGGAAGGAAGAGTAGGAAGTAAGTTAAGGAAAGTAGTAGGTAGTTACGTTTAAGGTAAAGGAAAACGTAAAAACCGTAAACCCGAAACCCCCAAAACCCTAAAACCTACCCCAACCTAACCCTAACCCAAAACGAAACGAACGAACGTACCTACCGACTA
TARGET=353,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_05992 Ps_contig_mira-and-tgicl-ass_franssen_40963
SEQUENCE=TATAGGTGAGGGGATAATGGAAAGGGTATATGAGGTATAATTTCCAAAGTGTTTGAAAGCAACTAAGCGCACAAAAAACCAATATCTATTCTTCAATTCATTCATTCAGAAGAACCAGAACGAAACGAATGATGCAAGGTTTATCTTCTTCATTATCATGCTCAATTCCATGTTCTTTAAAATTCAAAGCAGTGAAAGCTTCATTCGACACACAACAGAGACTCCCTTATAACCCAAACGCTCCTAAAAAACTCAACAACACTCAACCCCAATCGCAACCTCTTCCTCTCACTACATCATCACCACCGCCACTTTCTACGCGTAAGGACCAGTACATTGCCGATCTTCTCAAACGCGACACTCCTCTTCCCACCACCTCAGGGAAAATCAAAGAAGAAGAAGAAAGCGAAACCTATTTAGGTTATGAAAGATGGCTTCCAATTCCACCTAAAGTCGTTAAGCCTCGCTCGGTGTTTAATGCTACTACCTTAGCATATCTTGGTGATGGCATTTATGAGCTATATGCTCGTAGGCACTTTTTATTTCCTCCCCTTAGTATTGAAGAATACAATGATCGTGTGACGGCCGTGGTTCGCTGTGAGGCTCAAGATGCGTTGCTCCAGAAGCTTCTACACAGTAACTTCTTATCAGATCAAGAGAGGGATGTTCTTCGATGGGGAAAGAACATTGTTTCAAGCAAAACTAAGACAAAAAAGCGGGCTGGTTCAGTTGTATATAACAGGGCATCTTCACTAGAAACATTAGTTGGCTATCTCTATTTGACAAATGTGAATCGTTTGGAAAAGCTAATGTTAGAATTGGGATTCTCAATTGACTCTTCAATCCCTTTGAATTTGGAGGAACTAATAACAGGTGAGCTGAATAGTTGATAAAGTTGATCTTTGGGTATTCTTCCACTGGAGCAGGCAAAACAACTAATATAAAATTTTGTTCGATCTATTTCATCTTTCGGTAAAGCCTTTGAGATCAGAAGAAGCCTTGGAGAACGGTAGACATTGAAATGACATATTTTACTATGCCAGTAGGATCGAAATACTGAGACCTTTTGCAACAATGTATACATAGGTTATGGTATCATTTGTAATTGTTAGTATTGTTCTTCTTCAGAGTACAGTGAGAAGAAGAAAATTCAGTGAAGCTTTGGTTATTTGATAGATTTGTGTTAGGGTTAGCAACTCTGGTTGAACATGATCCAGCATTATAATTAATTGTGGAATTATGTTAGTTGAATACTATTGGAATACTTGGAATGATGATAACTTCGAATGGAGACTTCAAGTAATGG
TARGET=96,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8170
PRIMER_LEFT_PENALTY=0.567613
PRIMER_RIGHT_PENALTY=0.249378
PRIMER_LEFT_SEQUENCE=GAGGGGATAATGGAAAGGGTAT
PRIMER_RIGHT_SEQUENCE=AGCGTTTGGGTTATAAGGGAGT
PRIMER_LEFT=7,22
PRIMER_RIGHT=241,22
PRIMER_LEFT_TM=59.432
PRIMER_RIGHT_TM=60.249
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.8000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=0.8695
PRIMER_LEFT_1_PENALTY=0.567613
PRIMER_RIGHT_1_PENALTY=0.301926
PRIMER_LEFT_1_SEQUENCE=GAGGGGATAATGGAAAGGGTAT
PRIMER_RIGHT_1_SEQUENCE=GGGGTTGAGTGTTGTTGAGTTT
PRIMER_LEFT_1=7,22
PRIMER_RIGHT_1=269,22
PRIMER_LEFT_1_TM=59.432
PRIMER_RIGHT_1_TM=60.302
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.9109
PRIMER_LEFT_2_PENALTY=0.567613
PRIMER_RIGHT_2_PENALTY=0.343305
PRIMER_LEFT_2_SEQUENCE=GAGGGGATAATGGAAAGGGTAT
PRIMER_RIGHT_2_SEQUENCE=GGAGTCTCTGTTGTGTGTCGAA
PRIMER_LEFT_2=7,22
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=59.432
PRIMER_RIGHT_2_TM=60.343
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.8000
PRIMER_RIGHT_2_END_STABILITY=8.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=218
PRIMER_PAIR_PENALTY_3=0.9208
PRIMER_LEFT_3_PENALTY=0.671427
PRIMER_RIGHT_3_PENALTY=0.249378
PRIMER_LEFT_3_SEQUENCE=TAGGTGAGGGGATAATGGAAAG
PRIMER_RIGHT_3_SEQUENCE=AGCGTTTGGGTTATAAGGGAGT
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=241,22
PRIMER_LEFT_3_TM=59.329
PRIMER_RIGHT_3_TM=60.249
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=240
PRIMER_PAIR_PENALTY_4=0.9279
PRIMER_LEFT_4_PENALTY=0.567613
PRIMER_RIGHT_4_PENALTY=0.360320
PRIMER_LEFT_4_SEQUENCE=GAGGGGATAATGGAAAGGGTAT
PRIMER_RIGHT_4_SEQUENCE=ATCATTCGTTTCGTTCTGGTTC
PRIMER_LEFT_4=7,22
PRIMER_RIGHT_4=132,22
PRIMER_LEFT_4_TM=59.432
PRIMER_RIGHT_4_TM=60.360
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.8000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_05993 Ps_contig_mira-and-tgicl-ass_franssen_40963
SEQUENCE=TATAGGTGAGGGGATAATGGAAAGGGTATATGAGGTATAATTTCCAAAGTGTTTGAAAGCAACTAAGCGCACAAAAAACCAATATCTATTCTTCAATTCATTCATTCAGAAGAACCAGAACGAAACGAATGATGCAAGGTTTATCTTCTTCATTATCATGCTCAATTCCATGTTCTTTAAAATTCAAAGCAGTGAAAGCTTCATTCGACACACAACAGAGACTCCCTTATAACCCAAACGCTCCTAAAAAACTCAACAACACTCAACCCCAATCGCAACCTCTTCCTCTCACTACATCATCACCACCGCCACTTTCTACGCGTAAGGACCAGTACATTGCCGATCTTCTCAAACGCGACACTCCTCTTCCCACCACCTCAGGGAAAATCAAAGAAGAAGAAGAAAGCGAAACCTATTTAGGTTATGAAAGATGGCTTCCAATTCCACCTAAAGTCGTTAAGCCTCGCTCGGTGTTTAATGCTACTACCTTAGCATATCTTGGTGATGGCATTTATGAGCTATATGCTCGTAGGCACTTTTTATTTCCTCCCCTTAGTATTGAAGAATACAATGATCGTGTGACGGCCGTGGTTCGCTGTGAGGCTCAAGATGCGTTGCTCCAGAAGCTTCTACACAGTAACTTCTTATCAGATCAAGAGAGGGATGTTCTTCGATGGGGAAAGAACATTGTTTCAAGCAAAACTAAGACAAAAAAGCGGGCTGGTTCAGTTGTATATAACAGGGCATCTTCACTAGAAACATTAGTTGGCTATCTCTATTTGACAAATGTGAATCGTTTGGAAAAGCTAATGTTAGAATTGGGATTCTCAATTGACTCTTCAATCCCTTTGAATTTGGAGGAACTAATAACAGGTGAGCTGAATAGTTGATAAAGTTGATCTTTGGGTATTCTTCCACTGGAGCAGGCAAAACAACTAATATAAAATTTTGTTCGATCTATTTCATCTTTCGGTAAAGCCTTTGAGATCAGAAGAAGCCTTGGAGAACGGTAGACATTGAAATGACATATTTTACTATGCCAGTAGGATCGAAATACTGAGACCTTTTGCAACAATGTATACATAGGTTATGGTATCATTTGTAATTGTTAGTATTGTTCTTCTTCAGAGTACAGTGAGAAGAAGAAAATTCAGTGAAGCTTTGGTTATTTGATAGATTTGTGTTAGGGTTAGCAACTCTGGTTGAACATGATCCAGCATTATAATTAATTGTGGAATTATGTTAGTTGAATACTATTGGAATACTTGGAATGATGATAACTTCGAATGGAGACTTCAAGTAATGG
TARGET=391,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1138
PRIMER_LEFT_PENALTY=0.079714
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=CAGTGAAAGCTTCATTCGACAC
PRIMER_RIGHT_SEQUENCE=TGGAAGCCATCTTTCATAACCT
PRIMER_LEFT=189,22
PRIMER_RIGHT=439,22
PRIMER_LEFT_TM=59.920
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.1138
PRIMER_LEFT_1_PENALTY=0.079714
PRIMER_RIGHT_1_PENALTY=0.034102
PRIMER_LEFT_1_SEQUENCE=AGTGAAAGCTTCATTCGACACA
PRIMER_RIGHT_1_SEQUENCE=TGGAAGCCATCTTTCATAACCT
PRIMER_LEFT_1=190,22
PRIMER_RIGHT_1=439,22
PRIMER_LEFT_1_TM=59.920
PRIMER_RIGHT_1_TM=59.966
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.1654
PRIMER_LEFT_2_PENALTY=0.159680
PRIMER_RIGHT_2_PENALTY=0.005674
PRIMER_LEFT_2_SEQUENCE=CTTTCTACGCGTAAGGACCAGT
PRIMER_RIGHT_2_SEQUENCE=CGACTTTAGGTGGAATTGGAAG
PRIMER_LEFT_2=311,22
PRIMER_RIGHT_2=455,22
PRIMER_LEFT_2_TM=59.840
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=0.1654
PRIMER_LEFT_3_PENALTY=0.159680
PRIMER_RIGHT_3_PENALTY=0.005674
PRIMER_LEFT_3_SEQUENCE=ACTTTCTACGCGTAAGGACCAG
PRIMER_RIGHT_3_SEQUENCE=CGACTTTAGGTGGAATTGGAAG
PRIMER_LEFT_3=310,22
PRIMER_RIGHT_3=455,22
PRIMER_LEFT_3_TM=59.840
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.1772
PRIMER_LEFT_4_PENALTY=0.171564
PRIMER_RIGHT_4_PENALTY=0.005674
PRIMER_LEFT_4_SEQUENCE=TCTACGCGTAAGGACCAGTACA
PRIMER_RIGHT_4_SEQUENCE=CGACTTTAGGTGGAATTGGAAG
PRIMER_LEFT_4=314,22
PRIMER_RIGHT_4=455,22
PRIMER_LEFT_4_TM=59.828
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=142
=
PRIMER_SEQUENCE_ID=SSR_05994 Ps_contig_mira-and-tgicl-ass_franssen_40967
SEQUENCE=TTTAAATAATCCCATAAAAAAAGAAATAAAATAACAAAACAAAAAATAAACATTTTTGTCATATCCCAAACCATTTGATGCATTGGACTAAAAATTACTAATTTTAACAGCTAGTCAAAACAAATAAAAANTGTAATCAGGGAAGGATCCTGCGTCGCGGGCGTGGAGTCTCTCGCTTCACATACATTTTCTCAATATCATTCAGTGGTCGGGTTGATCCATCAGGCATGCTAGCAAATTTCCAGCCCCCGCCACCTCCTCGCTTCCCACGGGCTCCCATTTTTTCCACAGTAAAAGTCCATCCATTTTCAGGGCGTCGTCGACTATACTTGTGGCATTTCACCTCTCCAGCCAATTTTCTTCTTGTTGAAGTTCATCTTTTCAACAAAATCTCGATAAAGCATATCATCCTCCTTTTTGGAGATTTCATAATGAACTTCRTCCAAATCTTTAGTAATGCCATCCCACCCCTCAGGCATCACCTTGAAGTCGGGTTTATAAGGAAGAGACGCTACATGAAACTCCCTATCATGCGAAATAAAAAATTCAGGGAAAGTGTCGAGCAAGAGCTCAACGGAATCATCAAGACGGTGACCTAGCTTCTTCTCTTCTTCTTCTTCAAGCTGCTTAAGCCAAAGAATGGCATGAACCCTCTGTCTCATAATCCTGTAATCCTTAGCAAGCTTCTCAACAGTATAAACCTCAGGATCCTTTTTGTGCAAGCGATACATCTCAGCTTTCTCACTATCCTTCAAATACGATCCTCTCGCACCAGGCTCACGAACCTGTTTCAATAACACACACGTATCCGCCATTCGTTTCTTCCAACTATCAACATAAGATTTGCTCTTCCGATTCTCTTCTTCCAACTCCTGAAGCTTGTCTTCCATTTCTTGGAGATCCGAAAGAGTTGTTTGAGGATTCGCTTCGCCGTTGAAGTGGTCCTTTGTTAGGCCTGTTGACCAAGTTGAAGGTGTTTCCCAATTCGCGTCGTCGAGGGATCCTCCTCCGGGAGTTGTGGTTGAGAATGCTCTGGAAGGTGCCAAATGGAGGCGATTTTCGTGGCGGAGTTCGGTGGTGAGTCTTCGTGTGACGGAGATGAAACGGTTGATGGCTTGCATTGTAACGGCGTTGGGAATGGTGTCGATGGAGTGACGGCGACTGAGAGGGGAGAAAGGAAGGAATGGGAATGGTGTTAGTGTTCGTCTATGTCTATTGTGCAAATTGTAATTTATTTTAGTGTTCTGTTCTCCCCCCGA
TARGET=607,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05995 Ps_contig_mira-and-tgicl-ass_franssen_40985
SEQUENCE=TTTAAAGATCAATTGACCAATTTCTAATCCAGTAGACTTTTTTAATTCAATAATACATTGAAACCAATAACCCAATAGCCAAAACCACTTAACCAATCCAATTTTATTCAATATCCATTGTAATGTCTAATTGAGCCCCAAGCAAAAGCCCATAATCCAAATTCCCCAAGCCCAAGGATGGTTTCGAATAAGACNAAAAAAGAGTGAAGATGCAACATGGAGGAAACAACACCAAACGCAGGTAACGATAAAACGCAGCAGAAAGTTGAAAGATGCATAAGCTGCATAAGGCTACATAAAGCCAAACAGCTGCAACACAAGCCAAGCTTCCAAACGACACTCAAGCATCCAAACCAGTCTGAAGCCGCAACTGCATAAGCTGCAAACGCTCCAAGAAGCCAAGCCAAGCCACACCAAATTGAAAGAGAAAACAAAATCTAGGATTGAGGGGGGTTTTTCATCATTTATTTTCTTCACTCTCCATCGATTCTTCGTTCTTCAACCTTCTTTCTTCATCTTGTATCACATGCATCTTCATTCTGCCAAATCATCCCACTCGACAATCACTCAGTTCTGTAACCAAGCAATCTCCAAGTCTCACTCGTTTCATCCG
TARGET=396,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2026
PRIMER_LEFT_PENALTY=0.174518
PRIMER_RIGHT_PENALTY=0.028056
PRIMER_LEFT_SEQUENCE=ACTCAAGCATCCAAACCAGTCT
PRIMER_RIGHT_SEQUENCE=TGAAACGAGTGAGACTTGGAGA
PRIMER_LEFT=338,22
PRIMER_RIGHT=608,22
PRIMER_LEFT_TM=60.175
PRIMER_RIGHT_TM=60.028
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.3261
PRIMER_LEFT_1_PENALTY=0.174518
PRIMER_RIGHT_1_PENALTY=0.151533
PRIMER_LEFT_1_SEQUENCE=ACTCAAGCATCCAAACCAGTCT
PRIMER_RIGHT_1_SEQUENCE=AGAACGAAGAATCGATGGAGAG
PRIMER_LEFT_1=338,22
PRIMER_RIGHT_1=497,22
PRIMER_LEFT_1_TM=60.175
PRIMER_RIGHT_1_TM=59.848
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.3324
PRIMER_LEFT_2_PENALTY=0.180916
PRIMER_RIGHT_2_PENALTY=0.151533
PRIMER_LEFT_2_SEQUENCE=AGGCTACATAAAGCCAAACAGC
PRIMER_RIGHT_2_SEQUENCE=AGAACGAAGAATCGATGGAGAG
PRIMER_LEFT_2=288,22
PRIMER_RIGHT_2=497,22
PRIMER_LEFT_2_TM=59.819
PRIMER_RIGHT_2_TM=59.848
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=210
PRIMER_PAIR_PENALTY_3=0.3823
PRIMER_LEFT_3_PENALTY=0.174518
PRIMER_RIGHT_3_PENALTY=0.207756
PRIMER_LEFT_3_SEQUENCE=ACTCAAGCATCCAAACCAGTCT
PRIMER_RIGHT_3_SEQUENCE=AAGAACGAAGAATCGATGGAGA
PRIMER_LEFT_3=338,22
PRIMER_RIGHT_3=498,22
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=60.208
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=0.3823
PRIMER_LEFT_4_PENALTY=0.174518
PRIMER_RIGHT_4_PENALTY=0.207756
PRIMER_LEFT_4_SEQUENCE=ACTCAAGCATCCAAACCAGTCT
PRIMER_RIGHT_4_SEQUENCE=GAAGAACGAAGAATCGATGGAG
PRIMER_LEFT_4=338,22
PRIMER_RIGHT_4=499,22
PRIMER_LEFT_4_TM=60.175
PRIMER_RIGHT_4_TM=60.208
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=162
=
PRIMER_SEQUENCE_ID=SSR_05996 Ps_contig_mira-and-tgicl-ass_franssen_40993
SEQUENCE=AATTAACACCACTAATCAATATTAATAACTAATTCAAAAATACACACAATAAATTATTAATAAATCCCAAACATACTATTTCCACAACATTACTAAAGAAACCAATCAATTTTACAAACAAGTAAAATCAAATGTTACAAAAGAAATGAAATTGAAAATAACCCTACACATACAATACTAGACATGACTAAGCTCAAACCCTAAAACACCCTTAAGAACATCAACTAGTTGAAGTGGAGCAGTTAATTGAGGAAAATGTCCAAAAGTGTCAATTACCTCCAATGTAACTTTCCCTTTAATTTTCTTCTCCATATAAAGTGCTACACTATATGGAACAGCCATGTCACTTGAGGTTTGAATAATTGTGCATGGAGTTTCAACTTTCTCAAGTATATCTCTATAGTCACTGCAAAACACTGTCTTGGCTAAGGACAAAGAAACTTCATTTTTCATTCTTTTCAAAGACTCTCTGAATTTGTTCACTGATGATTCATCATTTGGATCCACTACATTTAAGGAGAAAGCACAAATCCAGTTTTCATAGTTTGATTCTATGTTCTCTAATAAATTCTCAATATCTGAACTTGCAAATCCTCCCTCATAATCATCTGTATTTATATATCTTGGGGAAGCACCAAGAAGAACAAGCCTCTTGAAAAGTTGAGGTCTTTTAATGGAAGCTAAACAACCAATCATACCAGACATAGAATGGCCAACAAAGGTAACATCTTTAAGCTTCATTTCATCCAATAAACTGATTAAATCATCTGCAAAAGCTTCTAATGAAGAATACTTCAAAGGGTCATAAAGATTTTCATCTTTAACACAAGCAGAAAAAGCCCAATCAAACAGAAGAATTTTGTAGGATTTTTCACTAAAACAAGGTGTGATTTTGTCCCATATTGATTGGTCTGTTCCATAACCATGAGCAAAAACTATTGTTTCTGTTCCTGATCCTATGATTCTAGCATTCAGATGAGTTGAAAGAAGATTCTCATGTTGTTCTATCATGTCGTTTGGTAATAATGGTTCCATAAAGAAAGAAAGAAAAATGAATGGAAGAGAAGAAAGGTAACCAACCAC
TARGET=1036,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0165
PRIMER_LEFT_PENALTY=0.000037
PRIMER_RIGHT_PENALTY=2.016476
PRIMER_LEFT_SEQUENCE=GTCTGTTCCATAACCATGAGCA
PRIMER_RIGHT_SEQUENCE=TGGTTACCTTTCTTCTCTTCCA
PRIMER_LEFT=909,22
PRIMER_RIGHT=1077,22
PRIMER_LEFT_TM=60.000
PRIMER_RIGHT_TM=57.984
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=169
PRIMER_PAIR_PENALTY_1=2.0201
PRIMER_LEFT_1_PENALTY=0.003589
PRIMER_RIGHT_1_PENALTY=2.016476
PRIMER_LEFT_1_SEQUENCE=CTGTTCCATAACCATGAGCAAA
PRIMER_RIGHT_1_SEQUENCE=TGGTTACCTTTCTTCTCTTCCA
PRIMER_LEFT_1=911,22
PRIMER_RIGHT_1=1077,22
PRIMER_LEFT_1_TM=59.996
PRIMER_RIGHT_1_TM=57.984
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=2.0375
PRIMER_LEFT_2_PENALTY=0.000037
PRIMER_RIGHT_2_PENALTY=2.037465
PRIMER_LEFT_2_SEQUENCE=GTCTGTTCCATAACCATGAGCA
PRIMER_RIGHT_2_SEQUENCE=GTTGGTTACCTTTCTTCTCTTCCA
PRIMER_LEFT_2=909,22
PRIMER_RIGHT_2=1079,24
PRIMER_LEFT_2_TM=60.000
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=2.0375
PRIMER_LEFT_3_PENALTY=0.000037
PRIMER_RIGHT_3_PENALTY=2.037465
PRIMER_LEFT_3_SEQUENCE=GTCTGTTCCATAACCATGAGCA
PRIMER_RIGHT_3_SEQUENCE=TGGTTGGTTACCTTTCTTCTCTTC
PRIMER_LEFT_3=909,22
PRIMER_RIGHT_3=1081,24
PRIMER_LEFT_3_TM=60.000
PRIMER_RIGHT_3_TM=60.037
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=173
PRIMER_PAIR_PENALTY_4=2.0411
PRIMER_LEFT_4_PENALTY=0.003589
PRIMER_RIGHT_4_PENALTY=2.037465
PRIMER_LEFT_4_SEQUENCE=CTGTTCCATAACCATGAGCAAA
PRIMER_RIGHT_4_SEQUENCE=GTTGGTTACCTTTCTTCTCTTCCA
PRIMER_LEFT_4=911,22
PRIMER_RIGHT_4=1079,24
PRIMER_LEFT_4_TM=59.996
PRIMER_RIGHT_4_TM=60.037
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=169
=
PRIMER_SEQUENCE_ID=SSR_05997 Ps_contig_mira-and-tgicl-ass_franssen_40994
SEQUENCE=CATCTGGCTGGCTCTCCGAAATGACATAACCTTGTGAGAAGTTCTGGCAACTTGAYGAGCCTGGTTGTCTAGAACTGAACCCCTCATTTCCTGAGCCTTTTTCTGAGCGAATCTCAGTTTGTTCATAATCTTGTCCATGGAAGATGCTCGCTTCTTTTCTAACTTCATCTCAAGTTTCCGTATTGCTGCCTCAGCTTTCGCCTTTTGCAAGTTCTCCCATGCAGTGATTTTTGCTTCCTCCCTTTTAGCCTTTGAAATAGTCTTTGAGCTTTCAGAAATTTCCCAAGATGCAGATCGAGTGCTTGTTTCTTTCTTTTCCAGCTGTCAACACTCTCTGAACCTCTACCAATGAATAAGGCCCTGTGTTTCTTGGACCACCTTGTCATGGTTACACGTTCATCTACCTGCACATCCCTGATATCCATTTTAGAGAGAGAGGCATTCTGCAACTCGGTGACAGGCAGGGCAGGGGGAGTGGCAGCAGAGAAAGAAGTCCTCCTATTGGGAGAGGAGCATGAACTTCCCTCTGGGCTCA
TARGET=429,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_05998 Ps_contig_mira-and-tgicl-ass_franssen_41010
SEQUENCE=TGCAAAAACAACATATCCAAGCTACAATTTCCAATGCATCAGTAGCATCNTAAACTAACTAACATAACCTTTAGCATCACAATATCAAATCAACTAACTAACTATCTTAACCTAACATAATCCTTAAACTAACTTAACCTACTCAATACTAACTTCACTAATTAAATAATGCCAGCCGGACTGCATGGGGATCGACAAGGACCAAAAGTTAGTTATAACAACCATGCATCGATTCCACAAATACCAACGGAATTCTAACATAATTTTCACAATCCCTATGCATCAAAACCACCCATAAATATTCCTCAATATCCAATTATTGGCTCCCTATTCATTGATCA
TARGET=92,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2117
PRIMER_LEFT_PENALTY=0.102789
PRIMER_RIGHT_PENALTY=0.108929
PRIMER_LEFT_SEQUENCE=TTTCCAATGCATCAGTAGCATC
PRIMER_RIGHT_SEQUENCE=AGAATTCCGTTGGTATTTGTGG
PRIMER_LEFT=27,22
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=60.103
PRIMER_RIGHT_TM=60.109
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.4952
PRIMER_LEFT_1_PENALTY=0.386267
PRIMER_RIGHT_1_PENALTY=0.108929
PRIMER_LEFT_1_SEQUENCE=CAATTTCCAATGCATCAGTAGC
PRIMER_RIGHT_1_SEQUENCE=AGAATTCCGTTGGTATTTGTGG
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=255,22
PRIMER_LEFT_1_TM=59.614
PRIMER_RIGHT_1_TM=60.109
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=1.0418
PRIMER_LEFT_2_PENALTY=0.102789
PRIMER_RIGHT_2_PENALTY=0.939039
PRIMER_LEFT_2_SEQUENCE=TTTCCAATGCATCAGTAGCATC
PRIMER_RIGHT_2_SEQUENCE=GAATTCCGTTGGTATTTGTGGA
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=254,22
PRIMER_LEFT_2_TM=60.103
PRIMER_RIGHT_2_TM=60.939
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=228
PRIMER_PAIR_PENALTY_3=1.0418
PRIMER_LEFT_3_PENALTY=0.102789
PRIMER_RIGHT_3_PENALTY=0.939039
PRIMER_LEFT_3_SEQUENCE=TTTCCAATGCATCAGTAGCATC
PRIMER_RIGHT_3_SEQUENCE=TTCCGTTGGTATTTGTGGAATC
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=251,22
PRIMER_LEFT_3_TM=60.103
PRIMER_RIGHT_3_TM=60.939
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=1.0431
PRIMER_LEFT_4_PENALTY=0.102789
PRIMER_RIGHT_4_PENALTY=0.940300
PRIMER_LEFT_4_SEQUENCE=TTTCCAATGCATCAGTAGCATC
PRIMER_RIGHT_4_SEQUENCE=GGAATCGATGCATGGTTGTTAT
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=60.940
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=209
=
PRIMER_SEQUENCE_ID=SSR_05999 Ps_contig_mira-and-tgicl-ass_franssen_41024
SEQUENCE=TTAATTTTTAAACTTTAGAAATCCATATGGCATACCAATATCAAAACATGGTTTCACTGTTGAGTATTGTATATAGAATTNAGAAATGTAAGGCTTAACCTTTAGTTAAGTTGTGCTCACTTATGTATCATCTCTCTTTCCTTATCAAAATATGAACACACTTAATTTGGTAGCAAAAACATTTCCATCCATCATCAACCTTGTATACATAATCTCATATTATGAACATAAATGAACTACTTTTCCATGTTCTTAAAGCAAAAGTAATCACACAATGTGATCACTCAAGATAAAGTGTCCAGTCAAAACTCGAAGTATCTGGCTGAGTTTGAGATTCATCTTTAACTTTAGCCACTTCTGCAGGAGATTTTTCAGGTTCAACAGATGCCGAAACTTCAGCCTCGGAGCTCTCAGGTTGTTGAACAGATAATGATGCATCTGTCTCGGAGCTCTCGAGTTTTTGAACAGATAACGATGCATCCGTCTCAGAGCTCTCGAGTTTTTGAACAGATAACGATGCATCCGTCTCGGAGCTCTCGAGTTGTTGAACAGATAATGATGCATCCGTCTCGGAGCTCTCGAGTTTTTGAACAGATAACGATGCATCCGTCTCAGAGCTCTCAAGTTTTTGAACAGATAACGATGCATCCGTTTCAGAGCTTTCGGGTTGAACAGGTAATGATGGTTCCGTCTCGGTGGTAGACACGGGGGGAGCTTGAGTAATTTCTTGTGACTTGAACTCGTTGATGTAGTAACTAAGTGACGGGCCAGAGTATTCGACTGTTTTGACCTGAGGAGGAGTATTCTTACCAACTTGTAGAAGTATTGATGATGCTGCGGCGATTGAGATCAGCGCAAGACCAGCAGCAATCGCCGCTGTGTTCTGAGCTCCATCAGAAGAAGAACCAGATGTTCCTGAACTTATAGCACTTTCTGCTACATAAACTGCTGGCTCAACTGAATATATGTTTGTCTGTCCAGCAGTGTCCTTTTCAACATAGCCAGTCCAACCCTGCTTACGACGTGGTGGATATGTACCAACTACTTTCGTCTTCTCCTCGGCTAACCATTCCATACTCACCTTCCCAACTTCCTTCTCAGGGGCACATTCTTCTTGATTGCAATCAGACTCATCAGAAGAAGCTCTTACAATGAACATGCATGAACTTCTCTTGCAAATCTTTTGTTGCTTCAAGGAGAATCTCCTTGTGGAGAAAGCACATGTGTTGGCTGAAATTGTTGCTTGCAATGAACAAGCCATTATTGGTAATGAATCAGTGTGTGTGTGTGTGTTTGAAGTTTAAGGTAAAAAGAATCTTAAAAACACAGAAAAACAGAAGAAAATGGTTTTGGTTTGGTTTTTTGATGGAAAGAGAAGGTTGTGTGTTATGGATTGGTGATTGGTGGTTGAGTTTGAAGGGATATATCTACTTT
TARGET=1278,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2513
PRIMER_LEFT_PENALTY=0.243031
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=AATCTCCTTGTGGAGAAAGCAC
PRIMER_RIGHT_SEQUENCE=CTTCAAACTCAACCACCAATCA
PRIMER_LEFT=1199,22
PRIMER_RIGHT=1418,22
PRIMER_LEFT_TM=59.757
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=220
PRIMER_PAIR_PENALTY_1=0.2583
PRIMER_LEFT_1_PENALTY=0.243031
PRIMER_RIGHT_1_PENALTY=0.015253
PRIMER_LEFT_1_SEQUENCE=AATCTCCTTGTGGAGAAAGCAC
PRIMER_RIGHT_1_SEQUENCE=CACCAATCCATAACACACAACC
PRIMER_LEFT_1=1199,22
PRIMER_RIGHT_1=1398,22
PRIMER_LEFT_1_TM=59.757
PRIMER_RIGHT_1_TM=60.015
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=0.3677
PRIMER_LEFT_2_PENALTY=0.243031
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=AATCTCCTTGTGGAGAAAGCAC
PRIMER_RIGHT_2_SEQUENCE=TTCAAACTCAACCACCAATCAC
PRIMER_LEFT_2=1199,22
PRIMER_RIGHT_2=1417,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=219
PRIMER_PAIR_PENALTY_3=0.4640
PRIMER_LEFT_3_PENALTY=0.243031
PRIMER_RIGHT_3_PENALTY=0.220992
PRIMER_LEFT_3_SEQUENCE=AATCTCCTTGTGGAGAAAGCAC
PRIMER_RIGHT_3_SEQUENCE=TATCCCTTCAAACTCAACCACC
PRIMER_LEFT_3=1199,22
PRIMER_RIGHT_3=1423,22
PRIMER_LEFT_3_TM=59.757
PRIMER_RIGHT_3_TM=60.221
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=0.7241
PRIMER_LEFT_4_PENALTY=0.243031
PRIMER_RIGHT_4_PENALTY=0.481074
PRIMER_LEFT_4_SEQUENCE=AATCTCCTTGTGGAGAAAGCAC
PRIMER_RIGHT_4_SEQUENCE=CCAATCACCAATCCATAACACA
PRIMER_LEFT_4=1199,22
PRIMER_RIGHT_4=1403,22
PRIMER_LEFT_4_TM=59.757
PRIMER_RIGHT_4_TM=60.481
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_06000 Ps_contig_mira-and-tgicl-ass_franssen_41038
SEQUENCE=GCCGACCAGCCTCGGCCTCGCCGTATCGGTCCGGCGGGTTGACCCGACAACAACATTGGGCTATGAGTTGGGTTCAAAGACGCCACATGGTTTATAACTATTGCCAAGATTCCAAAAGAGATCATAGACTAACACCTGAATGTTGGGGTTAAAATAAAAATTTCAACAAAAAATAAATAAATTTAGGGATGATTATTTAGGTCCCTAAACAATTTGATGGTGTAATTTATTGTGTTGTTGTCTTAGAATTATTTTTCTTAAGACACTCTTCATTTTATAATGTTACTATCTTTCTTACTATGGTCCTTTTCATGATGATCAATGTTACTATCTTACTATGGTTCCTTTTCA
TARGET=171,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.3691
PRIMER_LEFT_PENALTY=0.482784
PRIMER_RIGHT_PENALTY=0.886273
PRIMER_LEFT_SEQUENCE=GGCTATGAGTTGGGTTCAAAGA
PRIMER_RIGHT_SEQUENCE=ACCATCAAATTGTTTAGGGACC
PRIMER_LEFT=58,22
PRIMER_RIGHT=220,22
PRIMER_LEFT_TM=60.483
PRIMER_RIGHT_TM=59.114
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=1.3875
PRIMER_LEFT_1_PENALTY=0.501224
PRIMER_RIGHT_1_PENALTY=0.886273
PRIMER_LEFT_1_SEQUENCE=GACAACAACATTGGGCTATGAG
PRIMER_RIGHT_1_SEQUENCE=ACCATCAAATTGTTTAGGGACC
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=220,22
PRIMER_LEFT_1_TM=59.499
PRIMER_RIGHT_1_TM=59.114
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=176
PRIMER_PAIR_PENALTY_2=1.4484
PRIMER_LEFT_2_PENALTY=0.562082
PRIMER_RIGHT_2_PENALTY=0.886273
PRIMER_LEFT_2_SEQUENCE=GCCAAGATTCCAAAAGAGATCA
PRIMER_RIGHT_2_SEQUENCE=ACCATCAAATTGTTTAGGGACC
PRIMER_LEFT_2=102,22
PRIMER_RIGHT_2=220,22
PRIMER_LEFT_2_TM=60.562
PRIMER_RIGHT_2_TM=59.114
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=9.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=119
PRIMER_PAIR_PENALTY_3=1.8658
PRIMER_LEFT_3_PENALTY=0.979555
PRIMER_RIGHT_3_PENALTY=0.886273
PRIMER_LEFT_3_SEQUENCE=AACAACATTGGGCTATGAGTTG
PRIMER_RIGHT_3_SEQUENCE=ACCATCAAATTGTTTAGGGACC
PRIMER_LEFT_3=48,22
PRIMER_RIGHT_3=220,22
PRIMER_LEFT_3_TM=59.020
PRIMER_RIGHT_3_TM=59.114
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=173
PRIMER_PAIR_PENALTY_4=2.0113
PRIMER_LEFT_4_PENALTY=1.125045
PRIMER_RIGHT_4_PENALTY=0.886273
PRIMER_LEFT_4_SEQUENCE=CAAAGACGCCACATGGTTTAT
PRIMER_RIGHT_4_SEQUENCE=ACCATCAAATTGTTTAGGGACC
PRIMER_LEFT_4=74,21
PRIMER_RIGHT_4=220,22
PRIMER_LEFT_4_TM=59.875
PRIMER_RIGHT_4_TM=59.114
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=9.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_06001 Ps_contig_mira-and-tgicl-ass_franssen_41040
SEQUENCE=TCGGGGAGGCCAGAGCCGGGGGAGCTCAAAAAACAATTATTTTCAACAGCTTTTTTTGTGGTTAAAAACTAACTAAAAAGGAAAAATCTTTATCCTTTACTTTATCATTAGTATCAATATAGTAATAATTTTTGAAAGGAAAAAGTNATTTAATTTTATANGAATGATTAATTAATTGATTTACTTTCGATAAACAGTTAGCTTCATTTGACCGAACAAGGAGAGGACGGAAACAACACAGAGAGATTCAAGGATCTCGACCCATTCTTTAATCCCCATTTTCAGAAAAACAGTGAGAAAATAGCTATCATTATTTAACCTAATGAATGAATGAATGATAACACCGCCATCATTACTTTCTCTCACCCTTGACTCAGCCGTTCACAATCTCTCCGACATCTCCGATCTCTCTCCCATCCCTGATCACATCCTCCTCGATCTCTTCCTTAGAATATTGAAAGCAGGAAAACTGACTGAAAAAGTTCTCAAACTGTTTATAGCAACCGGTAAGGACGAAGTAATCTCACTTGTTCAGGCATTGAATATTCAACATATTCTCACCCCTGTGCTTCCTACCAGATGTTCTGAGAAATTCTAAGATAAAATTGAAAGTATAGTTAGAAGTTTGCATCCAACTCATCAGTTTTAAAGTAAGGAGACCAATCTAGACTTTCGGTTTGCGAAATCATCTGAACTGTATCCCTCAAAAGATGTCTCAAGCAATTATCAACGACAACGAAGTTGACATTGTGATTGGTGCACTAAACTCTGACCTAACAACTTTCATGAATGAATG
TARGET=322,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2826
PRIMER_LEFT_PENALTY=0.252355
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=GACCCATTCTTTAATCCCCATT
PRIMER_RIGHT_SEQUENCE=TCAGTCAGTTTTCCTGCTTTCA
PRIMER_LEFT=258,22
PRIMER_RIGHT=476,22
PRIMER_LEFT_TM=60.252
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.3540
PRIMER_LEFT_1_PENALTY=0.252355
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=GACCCATTCTTTAATCCCCATT
PRIMER_RIGHT_1_SEQUENCE=AGAAAGTAATGATGGCGGTGTT
PRIMER_LEFT_1=258,22
PRIMER_RIGHT_1=360,22
PRIMER_LEFT_1_TM=60.252
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=103
PRIMER_PAIR_PENALTY_2=0.5121
PRIMER_LEFT_2_PENALTY=0.252355
PRIMER_RIGHT_2_PENALTY=0.259704
PRIMER_LEFT_2_SEQUENCE=GACCCATTCTTTAATCCCCATT
PRIMER_RIGHT_2_SEQUENCE=TCAATGCCTGAACAAGTGAGAT
PRIMER_LEFT_2=258,22
PRIMER_RIGHT_2=541,22
PRIMER_LEFT_2_TM=60.252
PRIMER_RIGHT_2_TM=59.740
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=284
PRIMER_PAIR_PENALTY_3=0.6188
PRIMER_LEFT_3_PENALTY=0.252355
PRIMER_RIGHT_3_PENALTY=0.366463
PRIMER_LEFT_3_SEQUENCE=GACCCATTCTTTAATCCCCATT
PRIMER_RIGHT_3_SEQUENCE=CGTCCTTACCGGTTGCTATAAA
PRIMER_LEFT_3=258,22
PRIMER_RIGHT_3=514,22
PRIMER_LEFT_3_TM=60.252
PRIMER_RIGHT_3_TM=60.366
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.6284
PRIMER_LEFT_4_PENALTY=0.598109
PRIMER_RIGHT_4_PENALTY=0.030276
PRIMER_LEFT_4_SEQUENCE=TAGCTTCATTTGACCGAACAAG
PRIMER_RIGHT_4_SEQUENCE=TCAGTCAGTTTTCCTGCTTTCA
PRIMER_LEFT_4=198,22
PRIMER_RIGHT_4=476,22
PRIMER_LEFT_4_TM=59.402
PRIMER_RIGHT_4_TM=60.030
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=279
=
PRIMER_SEQUENCE_ID=SSR_06002 Ps_contig_mira-and-tgicl-ass_franssen_41048
SEQUENCE=TCTTTTATTTATTTATTTGTTTCTCTTTTTTTTATTAAGTCGTTTGTTTTATTTATTTTATTTTTGTTATTTTCTCATTTTTATTATGTTGATTTTGATGTAATAAAATTATAACACTGTTGTAATATAAGAATAATATCTTGGTTTGTTGACCCTTGAGGTTATGGTGTAGCTAGGATGATTCTGGTTTATGCTGCTGCTA
TARGET=4,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06003 Ps_contig_mira-and-tgicl-ass_franssen_41054
SEQUENCE=GAATTTAAGTGAAGTTTCATTCGATGTGATTGAAAGTGATGAACAAGGTTCCGGCAAAGGAGAATCGTAAAAAGGTGTCGAATAAAAAGGCTGCCAAACCTCCAAGACCTCCGAAGGGTCCGTCGTTAGACGAAGCTGATTATAGGTTAATTAGGGAGTTATCTGAACTTGCGACGTTGAAGCGTGCAAGGGTGGAGCGGATGAAAGCGTTGAAGAAAATGAAGAATGCTAAACCAAATTCGTCCAATAGCACTAGCGCGTTGGCCTTGGTTTTCACTGTTCTCTTCTTCGTTGTGATAATCTTCCAAGGCATGTCATCTGGGAAAAGCTCAGTGGCAAGTTTCCAAGGATCTCCTATATCAGGAGGAGGAGGAGGTGGTGGTTTAATTTCGGTTCAGTACCAACTCAATCCATCCAGCGATTCAAATGCGCCGGGTTCAGAGTCCCCCAATTTTGTACAGCGGGTAGCAGGTTCAGATTTGAATGAAAAAGTGAGGAGAGATTCAGGTTAATGCAATGTTTAAGGAGAAAGAGAATGCCATCATCACTTTTGTGTATTTTGAGAAGAGATAGATGGCTTAGTTTTACTGTGATTCTCATACAAACTTTTGTTTTTGTCTCTCAATAATTATCAATTATTATTAATCTGTTTTTTTGTCTT
TARGET=362,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0775
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.045749
PRIMER_LEFT_SEQUENCE=AATCTTCCAAGGCATGTCATCT
PRIMER_RIGHT_SEQUENCE=GATGATGGCATTCTCTTTCTCC
PRIMER_LEFT=298,22
PRIMER_RIGHT=545,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=60.046
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=248
PRIMER_PAIR_PENALTY_1=0.1791
PRIMER_LEFT_1_PENALTY=0.031769
PRIMER_RIGHT_1_PENALTY=0.147295
PRIMER_LEFT_1_SEQUENCE=AATCTTCCAAGGCATGTCATCT
PRIMER_RIGHT_1_SEQUENCE=TGGATGGATTGAGTTGGTACTG
PRIMER_LEFT_1=298,22
PRIMER_RIGHT_1=416,22
PRIMER_LEFT_1_TM=59.968
PRIMER_RIGHT_1_TM=59.853
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=119
PRIMER_PAIR_PENALTY_2=0.2395
PRIMER_LEFT_2_PENALTY=0.031769
PRIMER_RIGHT_2_PENALTY=0.207756
PRIMER_LEFT_2_SEQUENCE=AATCTTCCAAGGCATGTCATCT
PRIMER_RIGHT_2_SEQUENCE=TGATGGCATTCTCTTTCTCCTT
PRIMER_LEFT_2=298,22
PRIMER_RIGHT_2=543,22
PRIMER_LEFT_2_TM=59.968
PRIMER_RIGHT_2_TM=60.208
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=246
PRIMER_PAIR_PENALTY_3=0.2510
PRIMER_LEFT_3_PENALTY=0.205226
PRIMER_RIGHT_3_PENALTY=0.045749
PRIMER_LEFT_3_SEQUENCE=GCCTTGGTTTTCACTGTTCTCT
PRIMER_RIGHT_3_SEQUENCE=GATGATGGCATTCTCTTTCTCC
PRIMER_LEFT_3=263,22
PRIMER_RIGHT_3=545,22
PRIMER_LEFT_3_TM=59.795
PRIMER_RIGHT_3_TM=60.046
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.2528
PRIMER_LEFT_4_PENALTY=0.031769
PRIMER_RIGHT_4_PENALTY=0.220992
PRIMER_LEFT_4_SEQUENCE=AATCTTCCAAGGCATGTCATCT
PRIMER_RIGHT_4_SEQUENCE=GTACAAAATTGGGGGACTCTGA
PRIMER_LEFT_4=298,22
PRIMER_RIGHT_4=458,22
PRIMER_LEFT_4_TM=59.968
PRIMER_RIGHT_4_TM=60.221
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_06004 Ps_contig_mira-and-tgicl-ass_franssen_41073
SEQUENCE=GGTGGTGGTGGTGGGTCCATTTCCATTTGAAGAAGAAAAGAGCGTTTTTCCACCACCGTTTCTTCTTTGACTTGTGATTCTTGTTTCTCGATTCTTCTTTTGAAATGGGTTTTTGTAGCTTGAAGTGTATTGAGTCTATGGATCTTGAAGATTCTCGTTTGTGCTTCATGGCTTCTTCTAATACCTGAAAATAGTTGATTTGAGGGTCGAAACCATAGTCACTGAAGTGTTGAGGGTCTGATAAGGGAAAAACTGGGGACTTGTTGGACATTTTGGTGATATGTGTTTGATGAAAATTTTCAGATTTTGTTGTTTTTATT
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06005 Ps_contig_mira-and-tgicl-ass_franssen_41080
SEQUENCE=TCGGGGGTGCTATGTAATTATGTTATCTGACAACCCTACCAATACCATCTCTCTTCTCTCCTTTCCCATAACTCTCTTTCTTTTTCTTCTCTAATCTTATCATCTCCTTTTTAAGTATCGCCTTACCCATTCATTCATTGATTCTCCTCTTTTTCATACAAAAGCCTTTCCACCTTTTGTCTTTGCTTCTTCTCAATCACACTACTACTTGCCTTCCCTTTATATTATATCCCTCCACAATCCTCCTACTATGCTAATTGCTTATGTGACAGTATCATCATGATAAGCTAGTCAAAGTGTAGAAGAAGTTATAGCTCTTAGAAGATTCATATAAATGATGACTCCAAACTTAAACTTGGAACCCGAAAATCCTTCCGAAGCGCCTCGCGATCTTGGCTCCATGACGCTTGACTTGACACTCAATATCAGTTCGAGTGATGGCGAGTTTAAGGCTTCAAGTGATGCTAGCAGTGAAGTGGGAGCTACTGCTGAAGCTCATGCTTCGCCGCCATCGGTAACTCCAAGGGTT
TARGET=128,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6460
PRIMER_LEFT_PENALTY=0.645218
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=TCTGACAACCCTACCAATACCA
PRIMER_RIGHT_SEQUENCE=AGGTGGAAAGGCTTTTGTATGA
PRIMER_LEFT=25,22
PRIMER_RIGHT=174,22
PRIMER_LEFT_TM=59.355
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=150
PRIMER_PAIR_PENALTY_1=0.8465
PRIMER_LEFT_1_PENALTY=0.645218
PRIMER_RIGHT_1_PENALTY=0.201297
PRIMER_LEFT_1_SEQUENCE=TCTGACAACCCTACCAATACCA
PRIMER_RIGHT_1_SEQUENCE=AGCAAAGACAAAAGGTGGAAAG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=186,22
PRIMER_LEFT_1_TM=59.355
PRIMER_RIGHT_1_TM=59.799
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.8465
PRIMER_LEFT_2_PENALTY=0.645218
PRIMER_RIGHT_2_PENALTY=0.201297
PRIMER_LEFT_2_SEQUENCE=TCTGACAACCCTACCAATACCA
PRIMER_RIGHT_2_SEQUENCE=AAGAAGCAAAGACAAAAGGTGG
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=190,22
PRIMER_LEFT_2_TM=59.355
PRIMER_RIGHT_2_TM=59.799
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=0.9127
PRIMER_LEFT_3_PENALTY=0.912004
PRIMER_RIGHT_3_PENALTY=0.000742
PRIMER_LEFT_3_SEQUENCE=TCGGGGGTGCTATGTAATTATG
PRIMER_RIGHT_3_SEQUENCE=AGGTGGAAAGGCTTTTGTATGA
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=60.912
PRIMER_RIGHT_3_TM=60.001
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.2000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.9200
PRIMER_LEFT_4_PENALTY=0.645218
PRIMER_RIGHT_4_PENALTY=0.274768
PRIMER_LEFT_4_SEQUENCE=TCTGACAACCCTACCAATACCA
PRIMER_RIGHT_4_SEQUENCE=AGAAGCAAAGACAAAAGGTGGA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=189,22
PRIMER_LEFT_4_TM=59.355
PRIMER_RIGHT_4_TM=60.275
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=165
=
PRIMER_SEQUENCE_ID=SSR_06006 Ps_contig_mira-and-tgicl-ass_franssen_41081
SEQUENCE=ATTTGCCAAAGTGGTATGCTGTAGTAGTTGTGCTCTTCATTTGCATTTATGTATCAGGGTATGCTTGGTCATGGGGTCCCCTTGGTTGGTTGGTACCTAGTGAGATTTTTCCCATTGGAGATTCGTTCGGCTGCTCAAAGTCTCAATGTATCGGTCAACATGTTCTTCACTTTCTTAGTTTCAACTAAATTTTCTTGACAATGCTTTGCACATGTAAGTTTGGTTTNTTCATTCTTCTTGCCTT
TARGET=82,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4854
PRIMER_LEFT_PENALTY=0.186309
PRIMER_RIGHT_PENALTY=0.299089
PRIMER_LEFT_SEQUENCE=TTGCCAAAGTGGTATGCTGTAG
PRIMER_RIGHT_SEQUENCE=TCTCCAATGGGAAAAATCTCAC
PRIMER_LEFT=2,22
PRIMER_RIGHT=120,22
PRIMER_LEFT_TM=60.186
PRIMER_RIGHT_TM=60.299
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.5475
PRIMER_LEFT_1_PENALTY=0.186309
PRIMER_RIGHT_1_PENALTY=0.361206
PRIMER_LEFT_1_SEQUENCE=TTGCCAAAGTGGTATGCTGTAG
PRIMER_RIGHT_1_SEQUENCE=AAGTGAAGAACATGTTGACCGA
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=171,22
PRIMER_LEFT_1_TM=60.186
PRIMER_RIGHT_1_TM=59.639
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=170
PRIMER_PAIR_PENALTY_2=0.6002
PRIMER_LEFT_2_PENALTY=0.301100
PRIMER_RIGHT_2_PENALTY=0.299089
PRIMER_LEFT_2_SEQUENCE=TGCCAAAGTGGTATGCTGTAGT
PRIMER_RIGHT_2_SEQUENCE=TCTCCAATGGGAAAAATCTCAC
PRIMER_LEFT_2=3,22
PRIMER_RIGHT_2=120,22
PRIMER_LEFT_2_TM=59.699
PRIMER_RIGHT_2_TM=60.299
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.1000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=0.6623
PRIMER_LEFT_3_PENALTY=0.301100
PRIMER_RIGHT_3_PENALTY=0.361206
PRIMER_LEFT_3_SEQUENCE=TGCCAAAGTGGTATGCTGTAGT
PRIMER_RIGHT_3_SEQUENCE=AAGTGAAGAACATGTTGACCGA
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=171,22
PRIMER_LEFT_3_TM=59.699
PRIMER_RIGHT_3_TM=59.639
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.1000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=169
PRIMER_PAIR_PENALTY_4=0.6646
PRIMER_LEFT_4_PENALTY=0.186309
PRIMER_RIGHT_4_PENALTY=0.478252
PRIMER_LEFT_4_SEQUENCE=TTGCCAAAGTGGTATGCTGTAG
PRIMER_RIGHT_4_SEQUENCE=TACATTGAGACTTTGAGCAGCC
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=149,22
PRIMER_LEFT_4_TM=60.186
PRIMER_RIGHT_4_TM=59.522
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=148
=
PRIMER_SEQUENCE_ID=SSR_06007 Ps_contig_mira-and-tgicl-ass_franssen_41093
SEQUENCE=CATGATGTTAGGCCATCTAGATCCTGACCGTTCATCCCTTCCAATGGCGTCTTTCATTGATTGCTCCGATGCAAAGGTCACGAATCCAAATCCTTCTCGATCTTCCAGTTTCACGATCAAGGATAACCTGAACAACAACATCAACAACAAAAAACACAAATCGCATCAGATCTCACTCAATTTCAGATGACATGAAATAGAAAACAAACAAAAACATGATAGTAACCGATCGATCTTCGCCTTTGATTCAAAGNTGAAGATGAACGTACCATTAGAATAATAAAAGATCAGAATAGTAACAAGTATCATAGATGAAGTAACGGAAACNGNTCAGAGAGTAACAGAACAGAACAGA
TARGET=340,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06008 Ps_contig_mira-and-tgicl-ass_franssen_41104
SEQUENCE=AAACCAAGATTAAAAGATTTCAAATGGTTGTTTACTTCTTTTGCAAGTAATGTCAAAGGATGGTCAAACAAAAGTATTTGGTACATAAACCAATAGGCAGGACTAGTCTGCATGCATGTCCATGGCAGAATCGTTGGAACTGAATGCAGTAGAGTCCCTGCTTTGATGGTGAAAAAAGATGGATTCAGTGTTCTGGTTTCCATTACCTGTGGAGGGACCGCCGCTAAGGTAGCTCCCTCCCTCGCCAACTTGATAATGCTGAAGACTTCGACGAACAGGACTTGGCAAAGCATTTGAAAACTCAAAACTCTTGGATTGATTATCACAGTGCTCCGACCCGAGTCCCTGTCCAACAACTGTTTGGCACGAAGAACCAGGTGATACTGTAAAGCCAGAACTAGCAACTTGTGTTGCTGRTTGTGGTGCTGCTCTAGGCGAAATGGATTGCTCCTCTCCAAAATAATCCAGTTCATTCTGAATGTAGTTAAGTATATCAAGYGTTGCAACTCTTGATCCACCCTCTTGCTGTCTCCAGATCCACTGATAAAGTTTTTCGAGGCTATGTCGTTGACCAGCTTGGAAGGATAGTTTCTGGTGATTAACGGCATGCATGTGAAGCTGGGAGAGAGAATTAGCCGCGGTACAAAACGAAGCGTACATGGTTCGATCGACTTCGTCGAGGGTGGAGGCTATGGATTTCCTCTTCTTCGCCATGGATGGAAANGGAAGGAACGGAGAAGAAGCGAGGGTTTGGTATTATTATGGAGGAAGCAATGTTGTTTGTTTGTTTGAGGGGAAAGARGAGAAAGGTGGTGTAGGAGTGAGGATACAAATTTAGGGCATGCGAGGCGCACGTGATTATTATTCGCTCGCGTGGTTCCATTGGATTAGGAGAG
TARGET=777,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06009 Ps_contig_mira-and-tgicl-ass_franssen_41105
SEQUENCE=ATACNACATTTTCATACATCATTTTTATACATTATATCTCTTTCAAATTAATCAGAGGTCTGAGTTCGAACAGTGTTACACATGAATAGGTTATAGGTTCAAACCCAACCTTAAACATACAACAGTATTAAATTCTCTTAAGTATATCTGGTTTCTATTGGAATATATCAATATTTAACTCTTACTTTGATAAAATTACTATTATCTTTCTATTCATATTTTTTCTTAAATATTTAATATTAATCACTCTAAAGTTGCTATAAAAAGGCCATCCGTTACCCTAGTTGAATGGCGTTGTTGTTGTTGTTGTTGGTAGGTTACAAAGATATTACAAACCTGTGCACACCGCACTTTCTCCATCCCCTTACTGCAAATATTCTACTTTCACACTTGTTCCCATACTCTGCTACTTTCACTTTTCTCACCTCAGCCTCTATTTTTCTGTACTTTCGTTCCTTCTTCCATTCCTCTTTGTCTCTATAAACATTCATGGATGAGGACATTCTCAACACTTTTGAAAGTGATGAGAGCAGCATGGACATGATGGCAATGATGATGCAAATGGAAAAGTTTCCTGAACTCCACCAGCCTTTTTATAACAACAATCACAATCATACTATCAATCTTAACAACGCACATAACGAGTTCCCTTATGGAACTTATCCGCAACCACCACTCTCTTATCCTCACCACCCAATGACACCGTCTCTTCAACACAACGGAGTTCAAATTCCATCCGGGAGAATCAACAATGCTTCGTTCTCGTCGTACTCCGATAAGAAGAACTCAATGGCGGCCATGAGAGAGATGATATTCCGTATGGCCGTTATGCAGCCGGTTAATATAGACCCGGAAACAATCAAGCCACCAAAGAGAAAGAACGTGAAGATTTCAAAGGATCCACAAAGTGTAGCAGCCAGACACAGAAGAGAAAGGATTAGCCAGAGAATAAGAATCTTGCAGAGATTAGTCCCCGGTGGAACTAAAATGGATACCGCTTCAATGTTAGATGAAGCAATACATTATGTGAAGTTCTTGAAGAAACAAGTTCAAACGTTGGAACAAGTTGGAGCAAACAGACCTTTGAATGTTGTTGGATTCCCAGGAACGATTTCTAATGGAAATGTTAACTACTCATCTTTTCTGAGAGGTTGTTCATCACCTTGTCAAATGGTGGGTCCCACTTCCAAACAAATGCTTAGTTGATAAAGGATAGAGAGAAATTTGATTTTTCTTGATATATGTCCAATATATATTTGAAATATTTATGCATATTATTGTTGGATATGGTTTTAAGGTTAATGTTGGAATATTGTAAGCAAGAGTTATAAGCTAATAATGAATATGTTTTTATTGAACCTCT
TARGET=294,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1312
PRIMER_LEFT_PENALTY=0.122257
PRIMER_RIGHT_PENALTY=0.008930
PRIMER_LEFT_SEQUENCE=GGTTCAAACCCAACCTTAAACA
PRIMER_RIGHT_SEQUENCE=CAGTAAGGGGATGGAGAAAGTG
PRIMER_LEFT=95,22
PRIMER_RIGHT=369,22
PRIMER_LEFT_TM=60.122
PRIMER_RIGHT_TM=59.991
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.4216
PRIMER_LEFT_1_PENALTY=0.122257
PRIMER_RIGHT_1_PENALTY=0.299343
PRIMER_LEFT_1_SEQUENCE=GGTTCAAACCCAACCTTAAACA
PRIMER_RIGHT_1_SEQUENCE=ATATTTGCAGTAAGGGGATGGA
PRIMER_LEFT_1=95,22
PRIMER_RIGHT_1=376,22
PRIMER_LEFT_1_TM=60.122
PRIMER_RIGHT_1_TM=59.701
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=0.4466
PRIMER_LEFT_2_PENALTY=0.122257
PRIMER_RIGHT_2_PENALTY=0.324355
PRIMER_LEFT_2_SEQUENCE=GGTTCAAACCCAACCTTAAACA
PRIMER_RIGHT_2_SEQUENCE=TATTTGCAGTAAGGGGATGGAG
PRIMER_LEFT_2=95,22
PRIMER_RIGHT_2=375,22
PRIMER_LEFT_2_TM=60.122
PRIMER_RIGHT_2_TM=60.324
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=281
PRIMER_PAIR_PENALTY_3=0.4874
PRIMER_LEFT_3_PENALTY=0.478439
PRIMER_RIGHT_3_PENALTY=0.008930
PRIMER_LEFT_3_SEQUENCE=GCTATAAAAAGGCCATCCGTTA
PRIMER_RIGHT_3_SEQUENCE=CAGTAAGGGGATGGAGAAAGTG
PRIMER_LEFT_3=256,22
PRIMER_RIGHT_3=369,22
PRIMER_LEFT_3_TM=59.522
PRIMER_RIGHT_3_TM=59.991
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=0.5007
PRIMER_LEFT_4_PENALTY=0.478439
PRIMER_RIGHT_4_PENALTY=0.022302
PRIMER_LEFT_4_SEQUENCE=GCTATAAAAAGGCCATCCGTTA
PRIMER_RIGHT_4_SEQUENCE=ATGCTGCTCTCATCACTTTCAA
PRIMER_LEFT_4=256,22
PRIMER_RIGHT_4=535,22
PRIMER_LEFT_4_TM=59.522
PRIMER_RIGHT_4_TM=60.022
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.7000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=280
=
PRIMER_SEQUENCE_ID=SSR_06010 Ps_contig_mira-and-tgicl-ass_franssen_41110
SEQUENCE=TTTTAAAATAAACAAGAAGTAAATCCTCCCCCATTTAAATTACAACCGGATCTGAATGATTTATTTAGCACAAAGTAATTAGTGTAGTTATACAATATTGATGGTCATGCTGCTGCTACATCCATCTATATGAGGTTGGTCAGATGCAGTTATTTGGTCTAAAATTATAGTATCATTCAAAAAAATAACAATAACTAACTAAGATACAAATTAATAAGACATAGAATCTCAAAATGAGCAGCACAAGTACAGAAATCTGTTGGAGTGAGTCAGAATTCAAGCTTCTTGCATAGCTCCATTAGAATGGCCTTGGGTTGATGTTTGAGCTTGCGAGGCGCAGGAGGACAAGTATGAGCAATTCCTGTGAAACGGAGCTGGGGAGGAGGCGATTTACATTCATCTTGTTGCACATTGGACATTGGAGTGAGGAGCCTCTCCTCCATTTGCTTAGAAAGAATGAATTGAAAGAGACTGTCGTGTAGTGATTCAAAGATATCGAGATCGGAACGAGAATCAGCACGGAAAGGAAGACAGCGTACAACAGAGCTCCTGGAATCATTGAGATTCTTGCGAGTATCCGCAAAAGAGTCGACAAGAACATCCTTAGGGCCAGAATGAATGGTTGTCTGGTTAAGTTGTTTTCTGACGACGGTGAGTGTGAGAGGGGATTGCAATTCAAAATCAGCATTTTCCTTGACAACTTCAGGAGTTACAGGAACTTGACAATCTATTTTGTCACCATTGGGAGAGCTGGAATCCATATGGGGCGYAGTTTTAGTTCTGGGTTTGGTTGAGTTGAGTTGAGTTGAGTTGAGTTGAGTTGAAGAGGGAGACGGAAGAAAATGAATGAAACAGTTAAAGTTTTNTGAACNGTCGCCG
TARGET=790,35
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06011 Ps_contig_mira-and-tgicl-ass_franssen_41112
SEQUENCE=GAAACATCTTTCATTTTGTGCATTTATGCGAATACTTATATTAATTACAAACGTATATTAATCTAAAACTTTGTAGCCTTATTTAAACTTATAAACTCTAGTCTGGTTTCATATCTGTTGTTTCATTTCTTAACTTCCAAAACCAGTGTTTGACCTCCTTGTGTATTCTGCAACTAGTTGAGCAAGAGAAGAAGATTTGTCCTTAAGCAACTTCTTTGGTGAATCATATTCCTCAATAAGCCCTTTACTGAGAAACAAAACCATGTCGCTATCGAGGATTGAAGTTATTCTATGAGCAATGGTGATGACCGTGCAATCAGAGAAATGTTGCTTAACTGTCTGTTGAATTGTATTATCCATAGCCATATCAACTGATGCAGTAGCTTCATCGAGCACCAAGATCTCGCTTTTCTTAAGTAGAACACGGCCGAGGCAGACTAACTGCCTTTGACCCATGCTCCAGTTTTCTCCATTCTCAGTAACTGTGGAGTCAAGTTTTCCTTCTTTCTTTCTCACTTCATCTCCAAGTTGACACATATCAAGAG
TARGET=502,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.7608
PRIMER_LEFT_PENALTY=0.050443
PRIMER_RIGHT_PENALTY=2.710379
PRIMER_LEFT_SEQUENCE=AGGCAGACTAACTGCCTTTGAC
PRIMER_RIGHT_SEQUENCE=TGTGTCAACTTGGAGATGAAGT
PRIMER_LEFT=430,22
PRIMER_RIGHT=535,22
PRIMER_LEFT_TM=59.950
PRIMER_RIGHT_TM=57.290
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=2.9581
PRIMER_LEFT_1_PENALTY=0.247716
PRIMER_RIGHT_1_PENALTY=2.710379
PRIMER_LEFT_1_SEQUENCE=GTCGCTATCGAGGATTGAAGTT
PRIMER_RIGHT_1_SEQUENCE=TGTGTCAACTTGGAGATGAAGT
PRIMER_LEFT_1=264,22
PRIMER_RIGHT_1=535,22
PRIMER_LEFT_1_TM=59.752
PRIMER_RIGHT_1_TM=57.290
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=3.1097
PRIMER_LEFT_2_PENALTY=0.050443
PRIMER_RIGHT_2_PENALTY=3.059210
PRIMER_LEFT_2_SEQUENCE=AGGCAGACTAACTGCCTTTGAC
PRIMER_RIGHT_2_SEQUENCE=CTTGATATGTGTCAACTTGGAGATG
PRIMER_LEFT_2=430,22
PRIMER_RIGHT_2=542,25
PRIMER_LEFT_2_TM=59.950
PRIMER_RIGHT_2_TM=59.941
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=113
PRIMER_PAIR_PENALTY_3=3.2496
PRIMER_LEFT_3_PENALTY=0.539189
PRIMER_RIGHT_3_PENALTY=2.710379
PRIMER_LEFT_3_SEQUENCE=CCATAGCCATATCAACTGATGC
PRIMER_RIGHT_3_SEQUENCE=TGTGTCAACTTGGAGATGAAGT
PRIMER_LEFT_3=356,22
PRIMER_RIGHT_3=535,22
PRIMER_LEFT_3_TM=59.461
PRIMER_RIGHT_3_TM=57.290
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=3.3069
PRIMER_LEFT_4_PENALTY=0.247716
PRIMER_RIGHT_4_PENALTY=3.059210
PRIMER_LEFT_4_SEQUENCE=GTCGCTATCGAGGATTGAAGTT
PRIMER_RIGHT_4_SEQUENCE=CTTGATATGTGTCAACTTGGAGATG
PRIMER_LEFT_4=264,22
PRIMER_RIGHT_4=542,25
PRIMER_LEFT_4_TM=59.752
PRIMER_RIGHT_4_TM=59.941
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=279
=
PRIMER_SEQUENCE_ID=SSR_06012 Ps_contig_mira-and-tgicl-ass_franssen_41137
SEQUENCE=TTTTGATTGATTGAAGTCGAAAGCCTCGCTGCTGCTAGAAAAGCTGCTTTGTCTGGTTCTGAACCTTCAGATTCTATTCGGGTTGTCAATGCATTATTAACTCAGATGGATAAGCTTAAATCATCTCCAAATGTGATAATTCTAACCACATCCAACATTACCGCTGCTATCGATATTGCTTTTGTTGACCGAGCTGATATCAAAGCATATGTTGGTCCACCAACTCTTCAAGCTCGATATGAAATATTAAGGTCTTGCTTGCAGGAACTCATGCGTACAGGGATATTAACTAGTTTTGAGGACTGTAAGAATACCGTACTTCCAAATTATGCTAGTGCAAAACAAAGGATGAATGCACCCAATTTTCATGAGGCTACTACATCCATGCAACTGTGCAAGCAACTAGTTGAA
TARGET=3,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06013 Ps_contig_mira-and-tgicl-ass_franssen_41141
SEQUENCE=CCTCTAGTTCCAGAATCTTGTGATCCAGAATGGAGAGTGGTGATGGAGAGGTGCTGGTCTTCAGAACCATCAGAAAGACCTTCATTTACAGAGATTGCCAATGATTTACGTTTCATGGCTGCCAAGATCTCTCCCAAAGGACAAAATCAACAACAGCAACCTGCTTCATCTCAGAATCAAGTGCAGAAGTAATTTCCTGCAACTGCAGGAGCAAGATGCTTAATCCTATGTCCCTCTATGGTTTGTGTTTCCCATCCATTTGGTGGATGAATACGTTTTGCAATGTTTTCCATATGAAGGTCTCGTAAGTGTCAGAAGGTAACCTATGGTTGTTACCAAGTGCCAAGTCGTCTAGTAGCAGTACTACTACTACTACCTATTCTTGTAATATACCCAAACTTCACTGGACCCTGTAAGTTTTAGTTCGTTACTAGAAAAAGAARAAGAATAAAGAAAAATAGTTTGTTCATATTCTAAACTGTTTACAGTTGTAAAAATTGATATGGTAAAGAGTAGCAGCACTTTTAAAACG
TARGET=362,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06014 Ps_contig_mira-and-tgicl-ass_franssen_41144
SEQUENCE=CTGAAGATACAGAATGATTACTAGGAAATGAAAACGAAGGTCATTCATTCTGTTAATTTAAGGCATATTCATTCTTCTATCCATCCCACGACGCCGTTTTGACCTATCATTACAACTACTCGACCTATCCAACAACGACCTAAGCGAGTTCTGAAGAAAATGTATCGACTCAATGCTACTATCTTCTTTATCACCAGCAACAACAAGAACGTTCCGAGTTCTTCCTCCCAGTGTGCAAATTTCAGCTTTCACTGTTTTCAGCCGCAAAGATTTCAGAATTTCTATTAGCTCCGGGATTAGGTCCGACCGATCCTCGCAGCATAACGACGCTTTGAATATCAGTCTACCGTCTCCGCTGAAGTCTCCGCCCCCGCCGGAGATTACCGTTATTTCGTCGGTTTCCGATGGAACTGTTTCCAGTTCTGTCATCTCAGATGTTTGCTGCTTTAACTCTTTCACTCGTTCCACAACTTTTGCAAGAAGTGAAGCTTTGTCTGTCTTTGAATTGCAAGGGAGAAGAGTACGAAGATGATCAAGATGAGAATTGATTCTCTCTCTCCTTCTCTTCTCAGCCTCCTTATGATTCTTCAAAGCAGCAAGAGCTCTATCTTGTTGGGAGGAGGGTGTATCAGTGATTTCAGAAACTTCAAAAGGGTGATAGTAGTTGTTATTGTTGTTGTTGTTATCAGAAGAGCTACATAAACTTTGTTGCATCATAGGAGTATTAGAATAGTCAAAGAAGGAAGGGTTGTTAGCAACAATGAATTGAAAGAGTTCGGAGTTGTTTTCCATTGCAATATTTTGGTGAAGCATATCGTGAAAGAGAAGGAAAAGAGGGGGTGTGGATAGAGCTATAAGAATAGAAAACTATAATAAGAGAAAGTGAGTGGTAGAGTTCTTGAGTGTAAACATGTGGTGGTGAT
TARGET=550,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0539
PRIMER_LEFT_PENALTY=0.018835
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=GCAAGGGAGAAGAGTACGAAGA
PRIMER_RIGHT_SEQUENCE=TTGTTGCTAACAACCCTTCCTT
PRIMER_LEFT=507,22
PRIMER_RIGHT=760,22
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.2516
PRIMER_LEFT_1_PENALTY=0.216588
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=AATTGCAAGGGAGAAGAGTACG
PRIMER_RIGHT_1_SEQUENCE=TTGTTGCTAACAACCCTTCCTT
PRIMER_LEFT_1=503,22
PRIMER_RIGHT_1=760,22
PRIMER_LEFT_1_TM=59.783
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=0.2766
PRIMER_LEFT_2_PENALTY=0.241544
PRIMER_RIGHT_2_PENALTY=0.035021
PRIMER_LEFT_2_SEQUENCE=TTGAATTGCAAGGGAGAAGAGT
PRIMER_RIGHT_2_SEQUENCE=TTGTTGCTAACAACCCTTCCTT
PRIMER_LEFT_2=500,22
PRIMER_RIGHT_2=760,22
PRIMER_LEFT_2_TM=60.242
PRIMER_RIGHT_2_TM=60.035
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.2970
PRIMER_LEFT_3_PENALTY=0.261998
PRIMER_RIGHT_3_PENALTY=0.035021
PRIMER_LEFT_3_SEQUENCE=ATTGCAAGGGAGAAGAGTACGA
PRIMER_RIGHT_3_SEQUENCE=TTGTTGCTAACAACCCTTCCTT
PRIMER_LEFT_3=504,22
PRIMER_RIGHT_3=760,22
PRIMER_LEFT_3_TM=60.262
PRIMER_RIGHT_3_TM=60.035
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.3259
PRIMER_LEFT_4_PENALTY=0.136083
PRIMER_RIGHT_4_PENALTY=0.189810
PRIMER_LEFT_4_SEQUENCE=GAGATTACCGTTATTTCGTCGG
PRIMER_RIGHT_4_SEQUENCE=AGCTCTTGCTGCTTTGAAGAAT
PRIMER_LEFT_4=376,22
PRIMER_RIGHT_4=603,22
PRIMER_LEFT_4_TM=59.864
PRIMER_RIGHT_4_TM=59.810
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_06015 Ps_contig_mira-and-tgicl-ass_franssen_41144
SEQUENCE=CTGAAGATACAGAATGATTACTAGGAAATGAAAACGAAGGTCATTCATTCTGTTAATTTAAGGCATATTCATTCTTCTATCCATCCCACGACGCCGTTTTGACCTATCATTACAACTACTCGACCTATCCAACAACGACCTAAGCGAGTTCTGAAGAAAATGTATCGACTCAATGCTACTATCTTCTTTATCACCAGCAACAACAAGAACGTTCCGAGTTCTTCCTCCCAGTGTGCAAATTTCAGCTTTCACTGTTTTCAGCCGCAAAGATTTCAGAATTTCTATTAGCTCCGGGATTAGGTCCGACCGATCCTCGCAGCATAACGACGCTTTGAATATCAGTCTACCGTCTCCGCTGAAGTCTCCGCCCCCGCCGGAGATTACCGTTATTTCGTCGGTTTCCGATGGAACTGTTTCCAGTTCTGTCATCTCAGATGTTTGCTGCTTTAACTCTTTCACTCGTTCCACAACTTTTGCAAGAAGTGAAGCTTTGTCTGTCTTTGAATTGCAAGGGAGAAGAGTACGAAGATGATCAAGATGAGAATTGATTCTCTCTCTCCTTCTCTTCTCAGCCTCCTTATGATTCTTCAAAGCAGCAAGAGCTCTATCTTGTTGGGAGGAGGGTGTATCAGTGATTTCAGAAACTTCAAAAGGGTGATAGTAGTTGTTATTGTTGTTGTTGTTATCAGAAGAGCTACATAAACTTTGTTGCATCATAGGAGTATTAGAATAGTCAAAGAAGGAAGGGTTGTTAGCAACAATGAATTGAAAGAGTTCGGAGTTGTTTTCCATTGCAATATTTTGGTGAAGCATATCGTGAAAGAGAAGGAAAAGAGGGGGTGTGGATAGAGCTATAAGAATAGAAAACTATAATAAGAGAAAGTGAGTGGTAGAGTTCTTGAGTGTAAACATGTGGTGGTGAT
TARGET=671,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0539
PRIMER_LEFT_PENALTY=0.018835
PRIMER_RIGHT_PENALTY=0.035021
PRIMER_LEFT_SEQUENCE=GCAAGGGAGAAGAGTACGAAGA
PRIMER_RIGHT_SEQUENCE=TTGTTGCTAACAACCCTTCCTT
PRIMER_LEFT=507,22
PRIMER_RIGHT=760,22
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=60.035
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.1866
PRIMER_LEFT_1_PENALTY=0.151533
PRIMER_RIGHT_1_PENALTY=0.035021
PRIMER_LEFT_1_SEQUENCE=GAGCTCTATCTTGTTGGGAGGA
PRIMER_RIGHT_1_SEQUENCE=TTGTTGCTAACAACCCTTCCTT
PRIMER_LEFT_1=599,22
PRIMER_RIGHT_1=760,22
PRIMER_LEFT_1_TM=59.848
PRIMER_RIGHT_1_TM=60.035
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=162
PRIMER_PAIR_PENALTY_2=0.2516
PRIMER_LEFT_2_PENALTY=0.216588
PRIMER_RIGHT_2_PENALTY=0.035021
PRIMER_LEFT_2_SEQUENCE=AATTGCAAGGGAGAAGAGTACG
PRIMER_RIGHT_2_SEQUENCE=TTGTTGCTAACAACCCTTCCTT
PRIMER_LEFT_2=503,22
PRIMER_RIGHT_2=760,22
PRIMER_LEFT_2_TM=59.783
PRIMER_RIGHT_2_TM=60.035
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.2663
PRIMER_LEFT_3_PENALTY=0.231323
PRIMER_RIGHT_3_PENALTY=0.035021
PRIMER_LEFT_3_SEQUENCE=TGATTCTTCAAAGCAGCAAGAG
PRIMER_RIGHT_3_SEQUENCE=TTGTTGCTAACAACCCTTCCTT
PRIMER_LEFT_3=580,22
PRIMER_RIGHT_3=760,22
PRIMER_LEFT_3_TM=59.769
PRIMER_RIGHT_3_TM=60.035
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.2766
PRIMER_LEFT_4_PENALTY=0.241544
PRIMER_RIGHT_4_PENALTY=0.035021
PRIMER_LEFT_4_SEQUENCE=TTGAATTGCAAGGGAGAAGAGT
PRIMER_RIGHT_4_SEQUENCE=TTGTTGCTAACAACCCTTCCTT
PRIMER_LEFT_4=500,22
PRIMER_RIGHT_4=760,22
PRIMER_LEFT_4_TM=60.242
PRIMER_RIGHT_4_TM=60.035
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=261
=
PRIMER_SEQUENCE_ID=SSR_06016 Ps_contig_mira-and-tgicl-ass_franssen_41146
SEQUENCE=CGCAATCTCTGGTTTGAAAGTTTGTCTTTCTCACCCCATAAAAGAAATATTTGCTGTGGAAGTTTAGGGATGTTGATGTCTTTGTAGCTAATGACTAAGGCCTCTAATAATTCACTTCTCTCTTTCCTATTTGAGAACATCACCTTAATAAAATCACTGAGCAGACGATTGGGAAAAGGAATGTTTTTGTAGAAACCAACTGAAAGAAGTGTCCTCACTCCTTGTATAGAAGATGGCATCAACATTTCCGAACAAGAAGAAAACCCAGCATCTTCCACTGCCTTACTAATCATACACTCCGATATAGCCAAAACTGAACCAGTTACAACCACCGCTTGAACCAGTTCGTGATACATCTCAGCCATTTTAAACGCCACCATTCCACCGTAACTGAATCCAACCACAACACATTTCTCGACACCGAGTTTCTTCAACCCGGCCGCCACACACTCGGCCTGAAAAGCCAGTGACCGGTCCGGCTTATCGGATAAAGAACCACCGAAGAAAACTAAGTCGGGCACGTAAACCGCGTAGTCTTTGGCTAAAGGGCTGATTTGGAACTGCCATGTTGCAAGTCCGTCGCCGCAGAAGCCATGCAGAAGAACCACTACTGGCTTTGTAGGTTTCGAAATAGGTTTGTAGGATTTTGAAATGGTGTTAGAAGGAACCCAGAATCTCATTACTGTGCCTGGTTCTATCTCTACTGTGTAGAGCATTAAACCAGCCATTTTCACTACCCAACTAACGACGGACCAAATTACGTTCACCGTGTTCACCATGATTGTTTTTTCTCTCTCTCAAGTTTTATGTTTGCAGTGTGAAGTGAACTCT
TARGET=790,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4079
PRIMER_LEFT_PENALTY=0.023913
PRIMER_RIGHT_PENALTY=1.384026
PRIMER_LEFT_SEQUENCE=GAGCATTAAACCAGCCATTTTC
PRIMER_RIGHT_SEQUENCE=GAGTTCACTTCACACTGCAAACA
PRIMER_LEFT=710,22
PRIMER_RIGHT=829,23
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=60.384
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=1.5063
PRIMER_LEFT_1_PENALTY=0.122257
PRIMER_RIGHT_1_PENALTY=1.384026
PRIMER_LEFT_1_SEQUENCE=CTAAAGGGCTGATTTGGAACTG
PRIMER_RIGHT_1_SEQUENCE=GAGTTCACTTCACACTGCAAACA
PRIMER_LEFT_1=541,22
PRIMER_RIGHT_1=829,23
PRIMER_LEFT_1_TM=60.122
PRIMER_RIGHT_1_TM=60.384
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=289
PRIMER_PAIR_PENALTY_2=1.5200
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=1.384026
PRIMER_LEFT_2_SEQUENCE=ATGGTGTTAGAAGGAACCCAGA
PRIMER_RIGHT_2_SEQUENCE=GAGTTCACTTCACACTGCAAACA
PRIMER_LEFT_2=651,22
PRIMER_RIGHT_2=829,23
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=60.384
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=1.5449
PRIMER_LEFT_3_PENALTY=0.023913
PRIMER_RIGHT_3_PENALTY=1.520959
PRIMER_LEFT_3_SEQUENCE=GAGCATTAAACCAGCCATTTTC
PRIMER_RIGHT_3_SEQUENCE=AGTTCACTTCACACTGCAAACA
PRIMER_LEFT_3=710,22
PRIMER_RIGHT_3=828,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=58.479
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=119
PRIMER_PAIR_PENALTY_4=1.6429
PRIMER_LEFT_4_PENALTY=0.258908
PRIMER_RIGHT_4_PENALTY=1.384026
PRIMER_LEFT_4_SEQUENCE=AAATGGTGTTAGAAGGAACCCA
PRIMER_RIGHT_4_SEQUENCE=GAGTTCACTTCACACTGCAAACA
PRIMER_LEFT_4=649,22
PRIMER_RIGHT_4=829,23
PRIMER_LEFT_4_TM=59.741
PRIMER_RIGHT_4_TM=60.384
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=43.478
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_06017 Ps_contig_mira-and-tgicl-ass_franssen_41148
SEQUENCE=TTTAAAGCACTCCACATATTTATTCATTCATTCAACCCTTACATATTTTACATCACATAACTTGGATAATATTATCATAGGACAGAAACTAACTCCATATAATTCATACTATAGCTAGTAAAGTAGTAGCTAATTGGTTTTCAAACCCTGGTGCCTTTAAACTTCTTCTCAGCCTCCCTACTGTCCATGACTGCATCACGGTATCCATAGCGTCCTATATCACCCCTCCTGTAATAACCATCATCACCATAATCTCTACCATCATCAACGAATGTGACATGTATTGGTGAATTCCGTGGTCGTGATTGCAGCTGGTTAACATTTCCACTAGGCCTCATGATACCTGCAGAACCCGTCCTATCAGTAAAGCGAGGAGAACCACCCCAATTGTCCGGGGTTGGACTTGGACTATCGTAGTAGTGATGATGATCATTGTTTCTTTGACTGTGATCATGTTTTTCAGCTTCGATCATCAACAATTCCAAAGCTTTGTCAATATCATGCGTTGGCTCGCTTAGTTGGATCCCTTTTCTCGGTGCTGCACTCCAATCTGAACTAATTTTTGGTGCATTACCATAACTGTTACCATAGTTTCCATTCGTCACGGGCTTGTACCATTCACCATATCCATTAGGGCTATCATAGTAACCATTCTGAATGTTACCTCCTCCAATAGGTTTGTGCCCTTCTTTGTAGTCACCACCATATCCATTAGGGCTACCATAGTAGTCATTCTGAATGTTACCTCCTCCAACTGGTTTGTATCCTTCCTTGTCACCACTATATCCACTGTTATAGCCATCATGATTAGTCCTTATGCTACCTCCCCCAATAGGTTTGTGCCCTTCTTTGTAGAGACTCGGGTCTCCTACGGCTGGTGCTCCGCGGTGGTCACCACCATACCTATTAGGACTATCATACTCATAGTTGCCATTCTTAATGTTGCCTTCAATTGGCCTGCGTCCTTCTTTGTCACCACCACCATATCCGTTGTTATAGCCATCACGACTCGCACTCCTTATGCTACCTGTTCCCATTGGTTTGCGTTGTTCTTTATAAGGATTTGGATCT
TARGET=22,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06018 Ps_contig_mira-and-tgicl-ass_franssen_41164
SEQUENCE=AGTACGTCGGGGATCAGTCAAATTAATACTAATCCTCAAGCATATCTTATAGCAAAACTAATAGTTTTTTGAAATGGCTTCTAAGATTATTAGTGTTGCATTTTTCCTATGTCTCAATTTTTTCTTCATCACTATAGTGAACTCCAACTATGTTATCCCTGAAATTCCAGTGCCAATTCCAAACCCAACAATTGACCCTTATCCAAAGGCTACATGTCCTATAAATGCACTTAAATTAGGTGTGTGTGCAAATGTGTTGAACTTGGTGAAAGCTAAAGTGGGGTCTCCACCAACACTCCCTTGTTGCACCCTAATTAAAGGTCTTGCTAATCTTGAAGTTGCAGCATGCCTTTGCACTGCCCTTAAAGCTAATGTCCTTGGTCTTCACCTTGATGTTCCTATTGCTTTGAGTGTCATTCTCAACAATTGTGGATTGAACAACTCTGGTTTTCAATGCCCTTAGAATATTAATTTTTCATTGGCACCATATTTATAATTTCATGTGTTTCTTTGTCATATTTGTGTACTTTGTGTGTGTGTGTTTTTGTTGCAATATTCTTGACTTCTTACTCCTTCATTATTGTTCTTCTATTATATGGAAGATTATAAATCTTCTCCATCTGCTTTATCTATTGTATTAAAGATTTAGTACAAATAAAACTTTGTGGTTGACAAATTTCTCAATTRYATAYCTTGG
TARGET=530,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06019 Ps_contig_mira-and-tgicl-ass_franssen_41170
SEQUENCE=TAGGGGACGCACGTNCCANCACAAYATAGAACTACTACTATTTTTCTTCCAATTCACATTAATATTATTATTGAGAAACGAATGAAATACGGGGAAATTGGAATTCTTTGCTGTGCTACTCTTGTTGAGTTCATCTACTTGTTTAGCTTGGTTTGGTAGTAGTAAACACAAAACTTCAACTGCAAATGAACAATCCTCATCATCATCATCATCATCACCTTCGTTCTTAAACCGTTTTCGTTCTGGCTCATCCGTCGTGTTCCCGGTTCACGGAAATGTTTACCCTGTTGGTTTTTATAATGTGACTCTCAACATTGGGAATCCACCAAGGCCATATTTTCTTGATGTTGACACGGGTAGCGACCTAACATGGCTTCAATGTGATGCGCCTTGCTCCCGTTGCTCTCAGACACCCCATCCGCTTTACCGGCCTAGCAA
TARGET=197,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06020 Ps_contig_mira-and-tgicl-ass_franssen_41176
SEQUENCE=AATCAAAAATCCTAACGATCTCTTCCAACAACTGAAAGACACAACAAACTTTAAACATAAAAGTGTGTTTCCTAAAAGCAGGTTTTGAAGATTATTGGATGCTTGCGGTTCTAAACTTTGGTTTTTAGCTTCAAGTAATGGTATATGGGAGGAAAAAAATAAAGCTAGGGCTTTGATCTACAATATGAAACCTTGTTTTTCACCATTTTCTTGTATGGTGTTTAAAGCTTAATTTTCCTTTATAAGCAAAGAGAACATGAATTAACTATACCGTGTTCATGCAAATGTTGGTGAGTTTTGTTGGTGTTTTCTGTAGCTTTTTCTGGTGAAGTGATAGTTCAAAATGTAGCCATTGTTACTGTTATTTATATTCCATTTTTCAGTTGGATCTGAAAGTACTATATATATAGTGATGAATTTCAGTTGGAACNAGT
TARGET=400,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06021 Ps_contig_mira-and-tgicl-ass_franssen_41184
SEQUENCE=GGAAGGTGGCGAGGAACGGATTGTGGTGTATTTGACGAGTTTGAGGGGAATTCGGAGAACGTTTGAGGATTGTAATGCGGTGAAGATGATTCTGAAAGGGTTTAGGATTTGGGTGGATGAAAGAGATGTTTCCATGGATCGTGCTTTTAGAAAGGAATTGCAGTGTGTGATGGGAGAGGAAAATGTGTCACTACCTCAAGTCTTTATTAGAGGAAAATACATTGGTGGTGCTGATGTGATCAAGCATCTATTTGAAAGTGGTGATTTAAAAAGAATGCTTGAAGGGTTTCCCAAGATGAAGCCTGGTTTTGTTTGTGAGAGTTGCGGTGATGCTAGGTTTGTGCCATGTGAGAATTGCAGTGGAAGTCGAAAAATATTCGACGAAGACGAAGGTTTATTGAAGAGATGCTTGGAATGTAATGAAAATGGACTCACCAAGTGTCCTTCTTGCTCTTGTTATTGAATAACTTAGGTGATTATGATAATGGAGCGACGCGCCGGCGCCGGCGATGAAGTTGCAGTTGCAGATACGCTTCAAACTTTTATATTGAGATGAAATAAGATTGCAATGTTCTTGCAAGAGAATTTAAAATTGTTGATATTATGACTATAACTTAAACTTGATGATGATGATGATGTTTTTGCACCTTGAACATTGTTTTGTTTTGTTCAAGAGTGTTTTCTTTCTCACCATTTAAAATAAGAACAAAACTAGTTTCATTATTGAAACTTTCATAATGTGCATTTTGTGCATCTGTAAATATTTAGTGATGTATGAATAYGTAAATGTTTANRRTGATGAATATTCGTATTC
TARGET=622,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06022 Ps_contig_mira-and-tgicl-ass_franssen_41184
SEQUENCE=GGAAGGTGGCGAGGAACGGATTGTGGTGTATTTGACGAGTTTGAGGGGAATTCGGAGAACGTTTGAGGATTGTAATGCGGTGAAGATGATTCTGAAAGGGTTTAGGATTTGGGTGGATGAAAGAGATGTTTCCATGGATCGTGCTTTTAGAAAGGAATTGCAGTGTGTGATGGGAGAGGAAAATGTGTCACTACCTCAAGTCTTTATTAGAGGAAAATACATTGGTGGTGCTGATGTGATCAAGCATCTATTTGAAAGTGGTGATTTAAAAAGAATGCTTGAAGGGTTTCCCAAGATGAAGCCTGGTTTTGTTTGTGAGAGTTGCGGTGATGCTAGGTTTGTGCCATGTGAGAATTGCAGTGGAAGTCGAAAAATATTCGACGAAGACGAAGGTTTATTGAAGAGATGCTTGGAATGTAATGAAAATGGACTCACCAAGTGTCCTTCTTGCTCTTGTTATTGAATAACTTAGGTGATTATGATAATGGAGCGACGCGCCGGCGCCGGCGATGAAGTTGCAGTTGCAGATACGCTTCAAACTTTTATATTGAGATGAAATAAGATTGCAATGTTCTTGCAAGAGAATTTAAAATTGTTGATATTATGACTATAACTTAAACTTGATGATGATGATGATGTTTTTGCACCTTGAACATTGTTTTGTTTTGTTCAAGAGTGTTTTCTTTCTCACCATTTAAAATAAGAACAAAACTAGTTTCATTATTGAAACTTTCATAATGTGCATTTTGTGCATCTGTAAATATTTAGTGATGTATGAATAYGTAAATGTTTANRRTGATGAATATTCGTATTC
TARGET=656,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06023 Ps_contig_mira-and-tgicl-ass_franssen_41186
SEQUENCE=AACTAGAACAATAATTCAATAAAAATAAAAATTGCATACATCTAATATAAGAACTGATTTGATGGTTTGAGAAAATAAAGGGAAAAAAGGACTACGTATTACATTTTACAGGACAAATCTGTCCCAGTTCAAACACTACTAAAGAGTGCCTGTACCGCACAATCATCAATATGAGCTAAAAATCTGCTCGGCATAGTTGAGTGGTATCACTTTTGAAATTAAATCCTGAACTATTTACAAAATAGAAACATTGGTGTTCAAACAGCATTCATTGAAGTTGAAAGGACAACGTGAGCTAAAAATTGCACCCCGTCCTGGATTTTACTTATTGCTGTGGTTTGGTCAGCTCGAAGGTGTTGAGAGAAAGAATCGGCTTTTTCCTTAACTGATGATACCTTGGGAACATCTGTGGGAGACTCGGCAGTAACACCTTTAATGGCAGCTTGATAGGCCTCAGATACATCAGATATGCCTGTAATAAAACTGTTGGAAACTGCTTCCAAATATCCAATCATTGTCTGGGTATTTATCCTTATGATCTTAGCTTTTGCAGAAGCGTCTTCAGGCCACTCTATGTTAACCTTATCGGCGTCAATTTCTTCATCCTCAGTTTTGTTGGCAAGAGAAATGAGGGATTTGCTTAACGTAAGAAATTGTGACACCGCGAAACAGCACATTTCAGACAGTCTATGAACTCCCTCCGAATGAAGAGAGTCTATTCTTCCAGAAGTTCTCTGAATTATATCATTAACAGCTAGGCCAGTCAAGGCATTCGTGAAACCAGCAGCCATTTCGGCAGCCTTGCCAACACTGGAATCATGTAAATTCTTTATCTCATCACTGCTTCCCCCACTCTCTCTCTTTACTGTTTTTCCTTTGTTAGAATCAGCACCAGCATCAATTTCAGTACTCAAATCAAAAAATTTGTTGCACCTCTTTAAGCTTCCCTTCAAATAAAGATTTTTGCTCTGCANACTCTGCGTTGA
TARGET=853,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1522
PRIMER_LEFT_PENALTY=0.038361
PRIMER_RIGHT_PENALTY=0.113803
PRIMER_LEFT_SEQUENCE=GTCTATGAACTCCCTCCGAATG
PRIMER_RIGHT_SEQUENCE=TGAAGGGAAGCTTAAAGAGGTG
PRIMER_LEFT=685,22
PRIMER_RIGHT=951,22
PRIMER_LEFT_TM=59.962
PRIMER_RIGHT_TM=59.886
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.2093
PRIMER_LEFT_1_PENALTY=0.095538
PRIMER_RIGHT_1_PENALTY=0.113803
PRIMER_LEFT_1_SEQUENCE=TCATTAACAGCTAGGCCAGTCA
PRIMER_RIGHT_1_SEQUENCE=TGAAGGGAAGCTTAAAGAGGTG
PRIMER_LEFT_1=744,22
PRIMER_RIGHT_1=951,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=59.886
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=0.2467
PRIMER_LEFT_2_PENALTY=0.132857
PRIMER_RIGHT_2_PENALTY=0.113803
PRIMER_LEFT_2_SEQUENCE=GCCAACACTGGAATCATGTAAA
PRIMER_RIGHT_2_SEQUENCE=TGAAGGGAAGCTTAAAGAGGTG
PRIMER_LEFT_2=803,22
PRIMER_RIGHT_2=951,22
PRIMER_LEFT_2_TM=59.867
PRIMER_RIGHT_2_TM=59.886
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=0.2555
PRIMER_LEFT_3_PENALTY=0.030114
PRIMER_RIGHT_3_PENALTY=0.225403
PRIMER_LEFT_3_SEQUENCE=GAAATGAGGGATTTGCTTAACG
PRIMER_RIGHT_3_SEQUENCE=TACTGAAATTGATGCTGGTGCT
PRIMER_LEFT_3=624,22
PRIMER_RIGHT_3=908,22
PRIMER_LEFT_3_TM=59.970
PRIMER_RIGHT_3_TM=59.775
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=285
PRIMER_PAIR_PENALTY_4=0.2555
PRIMER_LEFT_4_PENALTY=0.030114
PRIMER_RIGHT_4_PENALTY=0.225403
PRIMER_LEFT_4_SEQUENCE=GAAATGAGGGATTTGCTTAACG
PRIMER_RIGHT_4_SEQUENCE=ATGCTGGTGCTGATTCTAACAA
PRIMER_LEFT_4=624,22
PRIMER_RIGHT_4=897,22
PRIMER_LEFT_4_TM=59.970
PRIMER_RIGHT_4_TM=59.775
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=274
=
PRIMER_SEQUENCE_ID=SSR_06024 Ps_contig_mira-and-tgicl-ass_franssen_41198
SEQUENCE=AATCAAAAGAATCGAGGTTAATTCTGTTTAATAAAAAAACAACTCGTAGTTTGTGGGTGGGCGTTGGGCGAATATACTATACTCCTATCTGCATTATCTTGTTTAGATAGTTATCTTATCGCAGGACCCATAGCCTGGCCTGGGGTTATCCTGTTTCAGCTCCATACAATGGCTCTGAATGCTACCGACTCAGAAACCAACAACAGCATCATCACCTTTCATTCAACAAATCCCCAATCACCATTTCTAATCGCACTCCCACTCTTCAAACTCCCCACTCCCCAACTCCCCACATCCATTTCCACCACATTTCATCAATGTTCCAAGTTTCTCCACTCTCTCGCTTCTCAAAACCCTATCTTAAACAAACTAATCTCTCTTTCCTCTCAATTCCATGACACATGCTTCCAGATTAGGTGCAGTAACTACAGGAATAGGAGGTTGGTGAATAGTCATCACAATTTCGCGGCTGTTTTACCTGGAGATTCAGTGGCTGGGTTAGTGGTGGCTAATGGACTTCAAAATTTCTTGAACCTTTACAATACTGTCATTGTTTGTAGGCTTGTTCTTACCTGGTTTCCCAATGCTCCTCCTGCTATCGTTGCTCCTCTCAGCACCGTATGTGACCCGTATCTGAACGTATTCCGTGGGCTTATTCCGCCTATTGGAGGATTGGATCTCTCTCCCATTCTAGCATTCCTTGTCCTGAATGCCTTCACCAGCACTGCTGCAGCACTCCCTGCTGAGCTTCCAATCACAGAACAATCTAAACAAGGTCCTGAAACAAGATTACAGTCTACCGATGTTACTTCTTCACAGAACAAATGGGTGAGGCGGCTTCAAGGGATCGGGTCAAAGACCTCCGCTACTGCAAATTAGAATATCATAATGTTTTCGTTTGTACCTCTGCCTGTTTATTTATTTAAATAGGTTTCTGTTGTAACTTCAGCTATCATAGTTCTAGGCAACACAATTTTCGAACCTGCATTCCTATGATTTTTTTTGTCAAAAGCTCTGAATTTATTATAATCATATCAATACCTTTTCTTTTCNNCTAG
TARGET=914,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3402
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.331942
PRIMER_LEFT_SEQUENCE=TTCTTCACAGAACAAATGGGTG
PRIMER_RIGHT_SEQUENCE=AAATCATAGGAATGCAGGTTCG
PRIMER_LEFT=809,22
PRIMER_RIGHT=998,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=60.332
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=190
PRIMER_PAIR_PENALTY_1=0.6082
PRIMER_LEFT_1_PENALTY=0.276218
PRIMER_RIGHT_1_PENALTY=0.331942
PRIMER_LEFT_1_SEQUENCE=ACCTCCGCTACTGCAAATTAGA
PRIMER_RIGHT_1_SEQUENCE=AAATCATAGGAATGCAGGTTCG
PRIMER_LEFT_1=858,22
PRIMER_RIGHT_1=998,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=60.332
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=141
PRIMER_PAIR_PENALTY_2=0.6082
PRIMER_LEFT_2_PENALTY=0.276218
PRIMER_RIGHT_2_PENALTY=0.331942
PRIMER_LEFT_2_SEQUENCE=AGACCTCCGCTACTGCAAATTA
PRIMER_RIGHT_2_SEQUENCE=AAATCATAGGAATGCAGGTTCG
PRIMER_LEFT_2=856,22
PRIMER_RIGHT_2=998,22
PRIMER_LEFT_2_TM=60.276
PRIMER_RIGHT_2_TM=60.332
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=143
PRIMER_PAIR_PENALTY_3=0.6364
PRIMER_LEFT_3_PENALTY=0.304436
PRIMER_RIGHT_3_PENALTY=0.331942
PRIMER_LEFT_3_SEQUENCE=GTTTTCGTTTGTACCTCTGCCT
PRIMER_RIGHT_3_SEQUENCE=AAATCATAGGAATGCAGGTTCG
PRIMER_LEFT_3=890,22
PRIMER_RIGHT_3=998,22
PRIMER_LEFT_3_TM=59.696
PRIMER_RIGHT_3_TM=60.332
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=109
PRIMER_PAIR_PENALTY_4=0.6987
PRIMER_LEFT_4_PENALTY=0.366796
PRIMER_RIGHT_4_PENALTY=0.331942
PRIMER_LEFT_4_SEQUENCE=CTTCACAGAACAAATGGGTGAG
PRIMER_RIGHT_4_SEQUENCE=AAATCATAGGAATGCAGGTTCG
PRIMER_LEFT_4=811,22
PRIMER_RIGHT_4=998,22
PRIMER_LEFT_4_TM=59.633
PRIMER_RIGHT_4_TM=60.332
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_06025 Ps_contig_mira-and-tgicl-ass_franssen_41200
SEQUENCE=TGGTATCAACGCAGAGTAGGGCGGCGCTTTTTTGAGCATTTTAAGTTTAACGTTTCTCCACTCTTCTCTCTTTCTCTACTCTCTCTCTCTTGTTTCCGAGTGTAGCAACAGAGAAAGAGCACAAACCAGATTCGAAGACAAAAATGGCGGGTTACAGAGCCGACGACGACTACGACTATCTCTTCAAGGTAGTTCTGATCGGCGACTCCGGCGTAGGTAAATCCAACCTTCTTTCTCGGTTCACGAAGAACGAGTTCAATCTCGAGTCCAAATCCACCATTGGTGTTGAATTCGCTACTCGTACTTTAAATGTTGATTCCAAAGTCATTAAGGCTCAGATCTGGGATACTGCTGGTCAAGAGAGGTATCGTGCCATCACCAGTGCTTACTATCGCGGGGCAGTTGGTGCACTTCTTGTATATGATGTCACCCGCCATGCAACATTTGAAAATGTCGACAGATGGCTGAAAGAACTGCGAAACCACACAGATTCCAACATTGTTGTGATGCTTGTTGGCAACAAAGCAGATCTCCGTCACCTTGTTGCCGTATCAACTGAAGACGGGAAGTCTTATGCTGAGAAAGAATCACTTTACTTTATGGAAACCTCAGCTCTCGAGGCTACTAATGTAGAGAACGCATTTGCTGAAGTTCTGACTCAAATCTACCATATCGTTAGCAAGAAAACGGTAGAGGGTGCTGAAAATGGAAACGCATCTGTCCCGGCCAAAGGAGAGAAAATAGATCTTAAAAATGATGTCTCTGCCTTGAAGAGAGTTGGTTGCTGCTCAAGCTAAGATTAGAAAACATTATTCGAAGTGGTTGCTATCGGCTCTGTTGTATGTGTAATATTCTAAGAGAGATACAAACTAGAAGAAAAAACAAAATAGTTAAATTTTAATTATTCATAGCTATTGTTAGTACT
TARGET=79,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1916
PRIMER_LEFT_PENALTY=1.139416
PRIMER_RIGHT_PENALTY=0.052187
PRIMER_LEFT_SEQUENCE=TGGTATCAACGCAGAGTAGGG
PRIMER_RIGHT_SEQUENCE=TCTCTGTTGCTACACTCGGAAA
PRIMER_LEFT=0,21
PRIMER_RIGHT=113,22
PRIMER_LEFT_TM=60.139
PRIMER_RIGHT_TM=60.052
PRIMER_LEFT_GC_PERCENT=52.381
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=114
PRIMER_PAIR_PENALTY_1=1.1916
PRIMER_LEFT_1_PENALTY=1.139416
PRIMER_RIGHT_1_PENALTY=0.052187
PRIMER_LEFT_1_SEQUENCE=TGGTATCAACGCAGAGTAGGG
PRIMER_RIGHT_1_SEQUENCE=TTCTCTGTTGCTACACTCGGAA
PRIMER_LEFT_1=0,21
PRIMER_RIGHT_1=114,22
PRIMER_LEFT_1_TM=60.139
PRIMER_RIGHT_1_TM=60.052
PRIMER_LEFT_1_GC_PERCENT=52.381
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=10.2000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=115
PRIMER_PAIR_PENALTY_2=1.1916
PRIMER_LEFT_2_PENALTY=1.139416
PRIMER_RIGHT_2_PENALTY=0.052187
PRIMER_LEFT_2_SEQUENCE=TGGTATCAACGCAGAGTAGGG
PRIMER_RIGHT_2_SEQUENCE=TTTCTCTGTTGCTACACTCGGA
PRIMER_LEFT_2=0,21
PRIMER_RIGHT_2=115,22
PRIMER_LEFT_2_TM=60.139
PRIMER_RIGHT_2_TM=60.052
PRIMER_LEFT_2_GC_PERCENT=52.381
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=1.1928
PRIMER_LEFT_3_PENALTY=1.139416
PRIMER_RIGHT_3_PENALTY=0.053348
PRIMER_LEFT_3_SEQUENCE=TGGTATCAACGCAGAGTAGGG
PRIMER_RIGHT_3_SEQUENCE=GGATTTGGACTCGAGATTGAAC
PRIMER_LEFT_3=0,21
PRIMER_RIGHT_3=274,22
PRIMER_LEFT_3_TM=60.139
PRIMER_RIGHT_3_TM=59.947
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=275
PRIMER_PAIR_PENALTY_4=1.2859
PRIMER_LEFT_4_PENALTY=1.139416
PRIMER_RIGHT_4_PENALTY=0.146525
PRIMER_LEFT_4_SEQUENCE=TGGTATCAACGCAGAGTAGGG
PRIMER_RIGHT_4_SEQUENCE=TTGTCTTCGAATCTGGTTTGTG
PRIMER_LEFT_4=0,21
PRIMER_RIGHT_4=140,22
PRIMER_LEFT_4_TM=60.139
PRIMER_RIGHT_4_TM=60.147
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_06026 Ps_contig_mira-and-tgicl-ass_franssen_41209
SEQUENCE=GACTGCAAAGTAAATAAAAAAACTATAATAAGCTGAACATTGCAAGTGCATGCTTCATTAAGAGAAACAAGAGTTGAAAATGGAGATTGAAGAAGTTTGTGAAACACAAGCACTTCCATTGCTCTCATTGAATCATGTCTCTCTTATGTGTAGATCAGTGTGGGAGTCTATGAAGTTTTATGAAGATGTTTTGGGCTTTGTTCCCATCAAAAGACCATCTTCTTTCAAATTTACTGGAGCATGGTTTTATAATTACGGTATTGGAATACACTTGATTCAGAATCCAGACGTTGATGAATTTGATACATATGTCAATGAGTCAAGGCCAATTAATCCTAAGGATAACCATATCTCCTTCCAGTGTACGGATGTTGAACTTGTTAAGAAGAGATTAGAAGAAAAGGGGATGAGATATGTTACAGCATTGGTAGAGGATGAAGGAATCAAGGTGGATCAAGTGTTCTTCCATGACCCTGATGGATACATGATTGAGCTTTGCAACTGTGAGAACATTCCAATCATTCCCATTTCTTCTTGCTCTGCCTCTTTCAAGCCTAGAAGCCATAGCTTTAAGAGGAGTCCTTCTAAGTGTGGATTCATAGAGAATGTTATGATGGAAAACTTGAGCTTGGACATGATGAACTTTGCCTTTTAAAAGTGAAGAACATTAATTCCACTTCATTTTTTGTAAGATAAGAAAAGTTTGTTCATCAAAACAAAACAAAAAACATGATAGGTTTTGGTGGATAATTACGAGTGTAAAATAGTTAGTCTTGGTTTAATTTTGAGGGAAGAGTGGTTCAAAGAAAAGTTGTATTGTATTTATATCATGTTTGTGTTTAAAAATAATATTAAGAAGTTGTGAGGTTGTTCTA
TARGET=712,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1401
PRIMER_LEFT_PENALTY=0.116582
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=GATGGAAAACTTGAGCTTGGAC
PRIMER_RIGHT_SEQUENCE=TGAACCACTCTTCCCTCAAAAT
PRIMER_LEFT=612,22
PRIMER_RIGHT=802,22
PRIMER_LEFT_TM=60.117
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.1747
PRIMER_LEFT_1_PENALTY=0.151106
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=CATTTCTTCTTGCTCTGCCTCT
PRIMER_RIGHT_1_SEQUENCE=TGAACCACTCTTCCCTCAAAAT
PRIMER_LEFT_1=525,22
PRIMER_RIGHT_1=802,22
PRIMER_LEFT_1_TM=60.151
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.2401
PRIMER_LEFT_2_PENALTY=0.216588
PRIMER_RIGHT_2_PENALTY=0.023556
PRIMER_LEFT_2_SEQUENCE=TTTCTTCTTGCTCTGCCTCTTT
PRIMER_RIGHT_2_SEQUENCE=TGAACCACTCTTCCCTCAAAAT
PRIMER_LEFT_2=527,22
PRIMER_RIGHT_2=802,22
PRIMER_LEFT_2_TM=59.783
PRIMER_RIGHT_2_TM=59.976
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=276
PRIMER_PAIR_PENALTY_3=0.2833
PRIMER_LEFT_3_PENALTY=0.259704
PRIMER_RIGHT_3_PENALTY=0.023556
PRIMER_LEFT_3_SEQUENCE=TTGAGCTTGGACATGATGAACT
PRIMER_RIGHT_3_SEQUENCE=TGAACCACTCTTCCCTCAAAAT
PRIMER_LEFT_3=622,22
PRIMER_RIGHT_3=802,22
PRIMER_LEFT_3_TM=59.740
PRIMER_RIGHT_3_TM=59.976
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=181
PRIMER_PAIR_PENALTY_4=0.2833
PRIMER_LEFT_4_PENALTY=0.259704
PRIMER_RIGHT_4_PENALTY=0.023556
PRIMER_LEFT_4_SEQUENCE=CTTGAGCTTGGACATGATGAAC
PRIMER_RIGHT_4_SEQUENCE=TGAACCACTCTTCCCTCAAAAT
PRIMER_LEFT_4=621,22
PRIMER_RIGHT_4=802,22
PRIMER_LEFT_4_TM=59.740
PRIMER_RIGHT_4_TM=59.976
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=182
=
PRIMER_SEQUENCE_ID=SSR_06027 Ps_contig_mira-and-tgicl-ass_franssen_41212
SEQUENCE=GGTCTTCAAAGCCAACTACAAAAAGCAACAGAAYNTCAGANTNCNYGNNNNAAGTCCTCATCACATTTTTCACTCAAACAAACAAAGAATCTTTCAACAAAAACCAATTATCACAATAATTACCATAAATCACAGAAAACAAATCAACAATAATCGAAACAGAAACGAAAAATTTCATAGCCATAAACCAAAAAAATAAAACTAAATATTTCAAAAAAAACTCACCGGTTTTTCTCCTCCGTTGCCGGAAACACAGCGCCGTCGTCATCCACCGCGTCCATTTTAAGTGAATTCAGAAGAAGAAAAGATGTCGAGGTTTGCATTCTTCAATCGATCAAATACAATTCACGCGTCTTCATCGGAAGAATCTCA
TARGET=75,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06028 Ps_contig_mira-and-tgicl-ass_franssen_41226
SEQUENCE=TATTTAGGGTTTAATTTGTATATATAGTGAATATAAAGTTTAATTATAAATATATAGGTGGACATACAATTGGTACAACTGCATGTCAATTCTTCAGTAACAGATTGAGAAACTTCACTACAAATGGTGCGGCTGATCCTTCCATTGACCCTTCATTTCTTTCCCAATTACAAACACTTTGCCCTCAAAACAGTGGTGCTACCAATAGAGTTGCATTGGATAATGGAAGTCAAAACAAATTTGATAATTCTTACTATGCTAATCTAAGGAACGGACGTGGAATTCTTCAATCTGATCAAGCACTGTGGAATGATGATTCAACAAAGACTTTTGTGCAGAGATATTTGGGTTTAAGAGGGTTGCTTGGATTAACATTTAATGTTGAATTTGGAAGATCAATGGTAAAGATGAGTAATATTGGAGTTAAGACAGGTACTGATGGTGAAATCCGCAAGATATGTTCTTCTTTCAACTAAGTGCTTATATAGTTTTCTTCATTAGTCTTTAGTGTTGGCATGCCTTCCATTTAAGTGTATAATACTTGGTTATAGTTTTTCTTTTCTTTTCATATCATAAGAAATATGTTGGAAGTGTAATAGTAATAAAAAAAGTGTGACATATATATCAATGRAAACTTCTTCGACTTATGAT
TARGET=553,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06029 Ps_contig_mira-and-tgicl-ass_franssen_41232
SEQUENCE=CACAAAATTGAAAAAAAGAAATATAGCTAATTTTCTTGTCTCAGATTTAACAACAAAAAAAGAATCGGAGGAGAAATTTCAATTTTCTCCCCTCTCTTGAAATTGAACAAAAAACAAAAGGAAGGAGAGCGCCAATGGGATCGCTCCACGTGTCTTCTCTCTCCCAATTGTCTGTTAGATCACAAGTGTAAGAAACGGGCAAGCTGAGTGGGGTTCACAACGCCGTGAAGGTGGGTCCCACCGCTCTTTAACAACCCTGTCACTTCCACGTACCTTTCACGCTCCATCTTTATCTCCGGCATCCGGCACGGCTCTTCCTCCGCAGTGGCAGTCGCAGTCGGTTCTCCTTCGTCACCGGCGTCGTCTTCTTCTTTGGACTTTCCCTTAGGTGGGACCCACGGTTGCCCTAAGAGAGTCCCCTTAGTGAGGTACTCGTGGGCCAGGTATCCCGCTAGCAGTTGATTGGACGGCATCGGCTCCAAACCTTTGTTAGCTGTTGTTGTTGTAGTAGTGGGTGGTGTAATCGGCTCCGACCCGAGCCGAGAAATAGGCTTATCCGACTTGGGTAATTTTGAAGTGGATGCGGGTATGGGCTTGGGCCTTTCAAACGG
TARGET=495,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2963
PRIMER_LEFT_PENALTY=0.078029
PRIMER_RIGHT_PENALTY=0.218249
PRIMER_LEFT_SEQUENCE=CGTCGTCTTCTTCTTTGGACTT
PRIMER_RIGHT_SEQUENCE=ATTACCCAAGTCGGATAAGCCT
PRIMER_LEFT=358,22
PRIMER_RIGHT=569,22
PRIMER_LEFT_TM=59.922
PRIMER_RIGHT_TM=60.218
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.3137
PRIMER_LEFT_1_PENALTY=0.095445
PRIMER_RIGHT_1_PENALTY=0.218249
PRIMER_LEFT_1_SEQUENCE=CCTTTCACGCTCCATCTTTATC
PRIMER_RIGHT_1_SEQUENCE=ATTACCCAAGTCGGATAAGCCT
PRIMER_LEFT_1=272,22
PRIMER_RIGHT_1=569,22
PRIMER_LEFT_1_TM=60.095
PRIMER_RIGHT_1_TM=60.218
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=298
PRIMER_PAIR_PENALTY_2=0.5920
PRIMER_LEFT_2_PENALTY=0.373775
PRIMER_RIGHT_2_PENALTY=0.218249
PRIMER_LEFT_2_SEQUENCE=TCTTTGGACTTTCCCTTAGGTG
PRIMER_RIGHT_2_SEQUENCE=ATTACCCAAGTCGGATAAGCCT
PRIMER_LEFT_2=369,22
PRIMER_RIGHT_2=569,22
PRIMER_LEFT_2_TM=59.626
PRIMER_RIGHT_2_TM=60.218
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.6131
PRIMER_LEFT_3_PENALTY=0.078029
PRIMER_RIGHT_3_PENALTY=0.535063
PRIMER_LEFT_3_SEQUENCE=CGTCGTCTTCTTCTTTGGACTT
PRIMER_RIGHT_3_SEQUENCE=GGATAAGCCTATTTCTCGGCTC
PRIMER_LEFT_3=358,22
PRIMER_RIGHT_3=557,22
PRIMER_LEFT_3_TM=59.922
PRIMER_RIGHT_3_TM=60.535
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=200
PRIMER_PAIR_PENALTY_4=0.6235
PRIMER_LEFT_4_PENALTY=0.078029
PRIMER_RIGHT_4_PENALTY=0.545451
PRIMER_LEFT_4_SEQUENCE=CGTCGTCTTCTTCTTTGGACTT
PRIMER_RIGHT_4_SEQUENCE=AATTACCCAAGTCGGATAAGCC
PRIMER_LEFT_4=358,22
PRIMER_RIGHT_4=570,22
PRIMER_LEFT_4_TM=59.922
PRIMER_RIGHT_4_TM=60.545
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_06030 Ps_contig_mira-and-tgicl-ass_franssen_41240
SEQUENCE=TCGGGGGTCTCNAAGGTAGNCAATCGGACACACACACTGAAACAGTCCTACTTTGGAGCTGAGCTAAACAACAATTACAATGGCAACGTCGTCGTCTAAGGCTTTCTCTAGGTTAACATCTCGATTACACTCTCTCCTTACAAAACCATCACCACTCTCCTCTCATCTCAAACCCAAACTTCAATCTGCAACTCTTCCACGCCTCTCTTTCACTTCACGGTTACCGGTGGAGTTGGGTAGCTTGGAATCAATGTTGCCGCTGTATACCGCTGTTGCTTCTGCTCGATTAGTTTCAAGCTTGTCCATTGAATCTATTGGTTGGGGTTTAGTTCCTCAAGGTATTTCCATGCCTTTATGACAGTGTTGATAGGCAGTTAGCTGATTCTAGTAGCATTTCCATTTAATGGAAGACTGTGGCTCATGTTGACGCTTTTCATTTCTATGTAATCTTGAGGCCTTTTGACATAGGATTCTTCATCTGATATCTTAGAGAGACATGCAATACAACTGATGTTGCTTACTCATTGAGTTGATTCATTTTATACTCAGAAGTTTCTAGAATATCTTTTTTTGTCTTTCCTATTTCTGGTATTTTTTTTAAAACTAATAATGCATTATTAAAGGTCATATTATATATCTGAACAATTTA
TARGET=28,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06031 Ps_contig_mira-and-tgicl-ass_franssen_41241
SEQUENCE=AGAAAATGGCAGTTTCACATTCGATCCTACGTCGACTCCGCTTGGCTTCTTCTTCTTCTTCATCACGCTTCACTAAACCATTTCACCTCTTCAAAGATTACACCAACAGAACATTGCAAGAGGACTAACAATCCAGAGTGCAGCCACAAAACCAGCTAAATCACCAGCTGAAGAAGAGTGGAAGGTTAAGCGAGAACTACTGCTGCAGAAAAGGGTAAAAAGTGTAGG
TARGET=46,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3844
PRIMER_LEFT_PENALTY=0.368190
PRIMER_RIGHT_PENALTY=0.016221
PRIMER_LEFT_SEQUENCE=CAGTTTCACATTCGATCCTACG
PRIMER_RIGHT_SEQUENCE=CTGCAGCAGTAGTTCTCGCTTA
PRIMER_LEFT=9,22
PRIMER_RIGHT=207,22
PRIMER_LEFT_TM=59.632
PRIMER_RIGHT_TM=59.984
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=7.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=199
PRIMER_PAIR_PENALTY_1=0.4005
PRIMER_LEFT_1_PENALTY=0.368190
PRIMER_RIGHT_1_PENALTY=0.032347
PRIMER_LEFT_1_SEQUENCE=CAGTTTCACATTCGATCCTACG
PRIMER_RIGHT_1_SEQUENCE=TGGTGATTTAGCTGGTTTTGTG
PRIMER_LEFT_1=9,22
PRIMER_RIGHT_1=165,22
PRIMER_LEFT_1_TM=59.632
PRIMER_RIGHT_1_TM=60.032
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=157
PRIMER_PAIR_PENALTY_2=0.4209
PRIMER_LEFT_2_PENALTY=0.368190
PRIMER_RIGHT_2_PENALTY=0.052729
PRIMER_LEFT_2_SEQUENCE=CAGTTTCACATTCGATCCTACG
PRIMER_RIGHT_2_SEQUENCE=CTTCCACTCTTCTTCAGCTGGT
PRIMER_LEFT_2=9,22
PRIMER_RIGHT_2=183,22
PRIMER_LEFT_2_TM=59.632
PRIMER_RIGHT_2_TM=60.053
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=175
PRIMER_PAIR_PENALTY_3=0.5398
PRIMER_LEFT_3_PENALTY=0.368190
PRIMER_RIGHT_3_PENALTY=0.171564
PRIMER_LEFT_3_SEQUENCE=CAGTTTCACATTCGATCCTACG
PRIMER_RIGHT_3_SEQUENCE=TTTTCTGCAGCAGTAGTTCTCG
PRIMER_LEFT_3=9,22
PRIMER_RIGHT_3=211,22
PRIMER_LEFT_3_TM=59.632
PRIMER_RIGHT_3_TM=59.828
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=203
PRIMER_PAIR_PENALTY_4=0.5881
PRIMER_LEFT_4_PENALTY=0.368190
PRIMER_RIGHT_4_PENALTY=0.219884
PRIMER_LEFT_4_SEQUENCE=CAGTTTCACATTCGATCCTACG
PRIMER_RIGHT_4_SEQUENCE=GTCCTCTTGCAATGTTCTGTTG
PRIMER_LEFT_4=9,22
PRIMER_RIGHT_4=124,22
PRIMER_LEFT_4_TM=59.632
PRIMER_RIGHT_4_TM=59.780
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=116
=
PRIMER_SEQUENCE_ID=SSR_06032 Ps_contig_mira-and-tgicl-ass_franssen_41249
SEQUENCE=CGCGGRCAAAAAAACGTAAGCTATTCGATGTGGCTTATGATGATGTGCAGTTTGCGGACACTGCAAATTCTCTAAAACGAAACCGACCCAGCGACCTCAATCAGAATGACGGTGATAACGACGATGCAGAATCAAGATGCTCTGGTAGCAATTATGGTCAAGGGTCTGGCGAAATGGGTTCTTTAGCAGACACTAAGAAGATGAGAAAAGAAAAGATTCAAAATGTTCTAAGCATTTTGCAGTGCATAGTACCTAATGGGAAGGATAAGGATCCAATCCAGCTTCTGGATGATGCCATACGCCGCTTGAAATCTTTGAAACTCAAGGCTAGAGCTCTCGGTCTCAACGCCTTGTAAATCGTTTCAAGAACATCAAGCCATCTACATTACTACTTATTACTTATATGTCATGTCTTAGCAGAATAAACAAGATGTGTTCATCTTTCACCAGAGGAAGTATTCAGCAAAAGTTCCGAAGTGTGGCGTATGAAATCCGATTATCTGCCAGCCTGAAACTGTGAGTTCCACACATAACCTTTTGAGAATTTCCTCAGTTGTTAATCTGGAACATAAAATTCAGCTGTTTTTTAATGCAACTTGTGTGTTATTACGTGTGAAGAAAAAGGGAAGTACTCACTATTACTCATCCAATTATGGCACAATAAAGGAAAAAAAGAATGATCAAGTCCATATCTTAGGGGTTAGAGAAAGTAGCACTAACGCAATAGCATGGGATATGAGCTGTTTTGAATGAGAAAACCTCAAAAAAACAAGAGTGCACGCCTCTTGGCTGTTATGGGACCCAACTTCACTCCCTCTTTTTGTATCCTTGTCATGTTCAGTCCTTTTGTGGGTTTACTTTTTTCCCTCATGTGTGCAATTTATTATCACTACCTAAGGCTATTAAGCACCACCACCACCCTTTTGTGAGAAGAAGACTGATACAGACAACTTTGTGTTGATGATGAGGGGACCCTTTTCATTCACTTCATGGATAGAGAAATTGGTGCAGTTATGTAAAAACTAAAAACACTGATTTTTATTTTACCTTGTATTGTTTTTTTGTTTAGTGTGTGTGTGTATTGTAATTGGTACTACTATAGTACTTATGATATCTTGGTTGAATGTAATTTAATGTGGTTGGTGTTANTGTTGTTCTTTTTRNNNTATGTATTTATATCGCATGCACGCTA
TARGET=908,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06033 Ps_contig_mira-and-tgicl-ass_franssen_41249
SEQUENCE=CGCGGRCAAAAAAACGTAAGCTATTCGATGTGGCTTATGATGATGTGCAGTTTGCGGACACTGCAAATTCTCTAAAACGAAACCGACCCAGCGACCTCAATCAGAATGACGGTGATAACGACGATGCAGAATCAAGATGCTCTGGTAGCAATTATGGTCAAGGGTCTGGCGAAATGGGTTCTTTAGCAGACACTAAGAAGATGAGAAAAGAAAAGATTCAAAATGTTCTAAGCATTTTGCAGTGCATAGTACCTAATGGGAAGGATAAGGATCCAATCCAGCTTCTGGATGATGCCATACGCCGCTTGAAATCTTTGAAACTCAAGGCTAGAGCTCTCGGTCTCAACGCCTTGTAAATCGTTTCAAGAACATCAAGCCATCTACATTACTACTTATTACTTATATGTCATGTCTTAGCAGAATAAACAAGATGTGTTCATCTTTCACCAGAGGAAGTATTCAGCAAAAGTTCCGAAGTGTGGCGTATGAAATCCGATTATCTGCCAGCCTGAAACTGTGAGTTCCACACATAACCTTTTGAGAATTTCCTCAGTTGTTAATCTGGAACATAAAATTCAGCTGTTTTTTAATGCAACTTGTGTGTTATTACGTGTGAAGAAAAAGGGAAGTACTCACTATTACTCATCCAATTATGGCACAATAAAGGAAAAAAAGAATGATCAAGTCCATATCTTAGGGGTTAGAGAAAGTAGCACTAACGCAATAGCATGGGATATGAGCTGTTTTGAATGAGAAAACCTCAAAAAAACAAGAGTGCACGCCTCTTGGCTGTTATGGGACCCAACTTCACTCCCTCTTTTTGTATCCTTGTCATGTTCAGTCCTTTTGTGGGTTTACTTTTTTCCCTCATGTGTGCAATTTATTATCACTACCTAAGGCTATTAAGCACCACCACCACCCTTTTGTGAGAAGAAGACTGATACAGACAACTTTGTGTTGATGATGAGGGGACCCTTTTCATTCACTTCATGGATAGAGAAATTGGTGCAGTTATGTAAAAACTAAAAACACTGATTTTTATTTTACCTTGTATTGTTTTTTTGTTTAGTGTGTGTGTGTATTGTAATTGGTACTACTATAGTACTTATGATATCTTGGTTGAATGTAATTTAATGTGGTTGGTGTTANTGTTGTTCTTTTTRNNNTATGTATTTATATCGCATGCACGCTA
TARGET=1069,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06034 Ps_contig_mira-and-tgicl-ass_franssen_41268
SEQUENCE=AAAGGAAAAAGAAAAGAGAAAACACACATTGCAATTTGGCGGTTCAAAAGTAGAGAATGGTGGTGGTGGATGAATGATTAGTGAACGTGAGAGTGAGCGAGTGTTCTTGAAGCATCCACAAACCAAAACAAAATCACCAATTTTCCCTTTTCACTCTCATTATTATTGCTACACACTTAGTATTGTCAGTTCAAAATAGGGTTTTCCCTCGCCACGAAACCCTAGTTACTCCGATAAAGAAGAGCAAAAAATGCCGAGAGAGATAATCACTCTTCAGGTAGGACAATGCGGGAACCAGATCGGTATGGAATTCTGAAAGCAGCTTTGTCTCGAACATGGCATCAGCAAAGACGGCATCCTCGAAGATTTCGCTACTCAGGGAGGAGATAGGAAAGACGTGTTCTTTTATCAAGCTGATGATCAGCATTATATACCGCGTGCTCTATTGATTGATTTGGAGCCTAGAGTTATTAATGGGATTCAAAATAGTGATTATCGAAATCTTTACAATCACGAGAACATCTTTGTTTCTGATCATGGAGGTGGTGCAGGAAACAATTGGGCTAGTGGATATGATCAGGGAAAGAATGTTGAAGAAGACATAATGGATATGA
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2786
PRIMER_LEFT_PENALTY=0.129103
PRIMER_RIGHT_PENALTY=0.149516
PRIMER_LEFT_SEQUENCE=TGGCGGTTCAAAAGTAGAGAAT
PRIMER_RIGHT_SEQUENCE=GAGTGAAAAGGGAAAATTGGTG
PRIMER_LEFT=36,22
PRIMER_RIGHT=155,22
PRIMER_LEFT_TM=60.129
PRIMER_RIGHT_TM=59.850
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=120
PRIMER_PAIR_PENALTY_1=0.4355
PRIMER_LEFT_1_PENALTY=0.333229
PRIMER_RIGHT_1_PENALTY=0.102276
PRIMER_LEFT_1_SEQUENCE=ATTTGGCGGTTCAAAAGTAGAG
PRIMER_RIGHT_1_SEQUENCE=GCTGCTTTCAGAATTCCATACC
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=322,22
PRIMER_LEFT_1_TM=59.667
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=6.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=290
PRIMER_PAIR_PENALTY_2=0.4578
PRIMER_LEFT_2_PENALTY=0.129103
PRIMER_RIGHT_2_PENALTY=0.328676
PRIMER_LEFT_2_SEQUENCE=TGGCGGTTCAAAAGTAGAGAAT
PRIMER_RIGHT_2_SEQUENCE=GAATTCCATACCGATCTGGTTC
PRIMER_LEFT_2=36,22
PRIMER_RIGHT_2=312,22
PRIMER_LEFT_2_TM=60.129
PRIMER_RIGHT_2_TM=59.671
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.4827
PRIMER_LEFT_3_PENALTY=0.333229
PRIMER_RIGHT_3_PENALTY=0.149516
PRIMER_LEFT_3_SEQUENCE=ATTTGGCGGTTCAAAAGTAGAG
PRIMER_RIGHT_3_SEQUENCE=GAGTGAAAAGGGAAAATTGGTG
PRIMER_LEFT_3=33,22
PRIMER_RIGHT_3=155,22
PRIMER_LEFT_3_TM=59.667
PRIMER_RIGHT_3_TM=59.850
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=123
PRIMER_PAIR_PENALTY_4=0.6541
PRIMER_LEFT_4_PENALTY=0.129103
PRIMER_RIGHT_4_PENALTY=0.524968
PRIMER_LEFT_4_SEQUENCE=TGGCGGTTCAAAAGTAGAGAAT
PRIMER_RIGHT_4_SEQUENCE=TCGGAGTAACTAGGGTTTCGTG
PRIMER_LEFT_4=36,22
PRIMER_RIGHT_4=233,22
PRIMER_LEFT_4_TM=60.129
PRIMER_RIGHT_4_TM=60.525
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=198
=
PRIMER_SEQUENCE_ID=SSR_06035 Ps_contig_mira-and-tgicl-ass_franssen_41270
SEQUENCE=ACATGATTCAATGAACTCTTAGCTAAAACATNTTAAACAGAGACAGAGCTATTCTTGACACAAACACAGACAACAAGAACCTTAAAATCATCCATGACATACTAGTACATATATGAACAATCTGCAACTAGCTTTTCTCGTGTACAGAGTCTATGTTAGTACTATTTGCAAACGCCTGGTTCACCCATTGAGGAAGTGGTGCACGCAGCATAACACGAATACCAGTAACAGGATGATCGAATGACAGCGTTTCAGCATGAAGGTGATGACCATCATGAACCCTACCTTTCCATTCATAAGCACCTTCATATTTCACGTCTCCAATTATTGAAATCCCTAAATATTGACAATGCAACCGAATCTGATGCGTTCTTCCACTCCGAGGATAAGCCCTCACCACTATCTCATTCCCATCACCACCGTCCATTTTCACCCCCTTTTCTTCAACGACAACCACACTACTCCCTTCAAACTCCGATTCACAAACCTCCCTGAAACTCCCTTTTCCGTTAATGGATAAAACCTCGAAAGAAGTTTCCATTTCACGTACACCCGATCCACCCGGTAACCCGCGACCGGCGTCGGAAAAAGCGTAAACTCTCCACGCGCCGAATTTTGACCTACCGTGACCGGATCTGACGGTTATTGTTTCCCAATCAGGTGCAACGCCGGTGCAGAGAGCGATGTAGGTTTTCTTGACTTTGTGATCGGTGAAGGCTTTGACGAGATTTGAAGCTACCTTGTGTGATTTTGTTATAAGCATAACGCCGCTTGTGTCGCGGTCGAGTCGATTAGCGAGATGAAGCTCCGGCGGTGCGGTTTTTTGTGATTGAAGGGAGGAGAGAACGCTGTCGCAGTAGATTCCTTGGGGTTTGTTGACTGCGAGGAGCCATTCGTCTTCGTAGATGGTGTGGGTTTTAGAGAGGGAGAAGAGGTCTGAGTTTGAAGAAGCGATTGTGGCTCTGTTTAGTTCGATTTGTTTTGAGATTGCGGGAAGTGGAGGGGATAATGGTATAGGGTAACTCTCGGCGATTATAGGGGTATTTACGGAAATATGAGATTGTTTGCTTTGGGGAGACGCTGACATGGCTACGGCGGCGGGTGAGGTTGAGCTGGGTGGCAGGTTGCACGGGTTTCTGAAATGGTGGTAGTGGTGGTGGTGGATATAGCATCCGGATCCGGATCCCACCCACATCGCCTGCTTCAGCATGGCCTCAGTGGGCCAAGTTTATTTATTCCTTTAGT
TARGET=1151,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.4667
PRIMER_LEFT_PENALTY=0.108206
PRIMER_RIGHT_PENALTY=1.358533
PRIMER_LEFT_SEQUENCE=GGTCTGAGTTTGAAGAAGCGAT
PRIMER_RIGHT_SEQUENCE=AATAAACTTGGCCCACTGAGG
PRIMER_LEFT=933,22
PRIMER_RIGHT=1232,21
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=60.359
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.8000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=1.4960
PRIMER_LEFT_1_PENALTY=0.295414
PRIMER_RIGHT_1_PENALTY=1.200595
PRIMER_LEFT_1_SEQUENCE=TCGGCGATTATAGGGGTATTTA
PRIMER_RIGHT_1_SEQUENCE=GGAATAAATAAACTTGGCCCAC
PRIMER_LEFT_1=1025,22
PRIMER_RIGHT_1=1238,22
PRIMER_LEFT_1_TM=59.705
PRIMER_RIGHT_1_TM=58.799
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.2000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=214
PRIMER_PAIR_PENALTY_2=1.6539
PRIMER_LEFT_2_PENALTY=0.295414
PRIMER_RIGHT_2_PENALTY=1.358533
PRIMER_LEFT_2_SEQUENCE=TCGGCGATTATAGGGGTATTTA
PRIMER_RIGHT_2_SEQUENCE=AATAAACTTGGCCCACTGAGG
PRIMER_LEFT_2=1025,22
PRIMER_RIGHT_2=1232,21
PRIMER_LEFT_2_TM=59.705
PRIMER_RIGHT_2_TM=60.359
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=208
PRIMER_PAIR_PENALTY_3=1.6813
PRIMER_LEFT_3_PENALTY=0.322785
PRIMER_RIGHT_3_PENALTY=1.358533
PRIMER_LEFT_3_SEQUENCE=AGCGATTGTGGCTCTGTTTAGT
PRIMER_RIGHT_3_SEQUENCE=AATAAACTTGGCCCACTGAGG
PRIMER_LEFT_3=949,22
PRIMER_RIGHT_3=1232,21
PRIMER_LEFT_3_TM=60.323
PRIMER_RIGHT_3_TM=60.359
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=1.7146
PRIMER_LEFT_4_PENALTY=0.514028
PRIMER_RIGHT_4_PENALTY=1.200595
PRIMER_LEFT_4_SEQUENCE=CTCGGCGATTATAGGGGTATTT
PRIMER_RIGHT_4_SEQUENCE=GGAATAAATAAACTTGGCCCAC
PRIMER_LEFT_4=1024,22
PRIMER_RIGHT_4=1238,22
PRIMER_LEFT_4_TM=60.514
PRIMER_RIGHT_4_TM=58.799
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=215
=
PRIMER_SEQUENCE_ID=SSR_06036 Ps_contig_mira-and-tgicl-ass_franssen_41273
SEQUENCE=CAGAGTACGAAGGGGAAACCCTAATGAACCAATACAACTACCGTCACCTCTCTAAACCACCGCCGCCGGCCCAACCACACCGTCTATTCCCTCCCACCGCCAGAATTCCAACCCATTCTCAACTGTAATTCTTCAGTTCAGCCTCAAATTGAAGCTGGTTCAGATTTAGTTGTTTAGAATATCTGCAAATTTTTGAGATACAAGTATGAATAACGAACCTCCTCCAAAGAGTACTAAATCACTGAAGAGAAAACGGAAGTCGATCAAGAAGAAGAAGAAGAAGGCAGAGGCGGCTAAAGAAAATGATAGTTTGAACAGTAACATTGTTTGTGAGTCTGAAAACTCTATCACAGAAGATAGGTCAAAGGCTTTGCCAGTTCCTTCCATTGGAAAATCTGTTGGATGTTTGAAGAAGAAGCTTCTTATTCTTGATATTAATGGGATTCTTGTTGACGTAGTTTCACATCCTTTCCCAAAGAAAATAAAAAGAGATGCAATGATAGCGAAGAAAGCATTATTCAAGAGGCCTTTTTGTAGTGAGTTTCTGAACTTTTGCTTCGAGAAATTTGACGTGGCTGTGTGGTCTTCAAGAATGGAGAAAAATGTCAACAGTATCATTAATCATTTGTTGGGAAACATGAGTCAGAGGTTGATTTTCTGTTGGAACATTTCACAATGTACTAAGACAAATATCAAAGCTCTCGGAAACAATCACAAGCTTGTTGTTTTTAAGGATCTGAGGAGAATTTGGGATAAGTATTATCCTGATCTTCCTTGGGAGAAGGGATACTACAACGAATCAAATACACTGCTTTTAGACGACTCTCCCTACAAGGGTTTACTCAATCCTCCGCACAATTCAATATATCCGCCAACATTCAGTTGTCGACAACTTAATGACAGCTCACTAGGTGTTGGAGGTCTCATACGAAATTATCTGGAGCGATTAGCGAATGCTGAAAATGTGTCGAAGTTTGTAGAGCAGAATCCATTTGGTCAAAAGCGAATCAGTGAGACGAGTGAATCTTGGAGCTTCTACTGCAATATACTTCATAGGCTTCGACCTAAAAATTGAAAATCAGGTGCCCTCTCAACTGGAATATCAATTTTGAAGAATTTGAGACAGAAGGCTCTTTCTCTCTGACTCTAACTCTAGTCAATAATACATAACTGTGTATTTTTGTGCATGATTGCACTTAAGGACCCTCTTTTCAATTTTATCACTGTTGTATTTTAACTATGATTTTCCTCCTTTTGGATAAATGTACTAGAGTTGAAGTATTTAAAAAAATAGTTCTTGTTAGTTAGAATTTTGCTGAAAAATGCAGAAGTGAGAAATNNARAYTTGGTTGTGTGGTGCAAAAAAAT
TARGET=266,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06037 Ps_contig_mira-and-tgicl-ass_franssen_41277
SEQUENCE=TTTGTATAAAGGGTTTAACGAATGACCTGCTAGATTGAGTTGAGGTTTGTCGACCATTTCCAAATGTGGATAGAAATGCTATGTTCACAATTTTGTTAGAGTCCAGTGTTGCATGTTTCTGTCAATGAACCTTCTCTCTCGTTTTGACCCCAATAAACCACTATGTCTCCAGCATTTGTAGTAGAAAACAAGGAGAGGAAAATCACGAGAGAGAGAATTGGATTTGAAAGGCTACATGTTTCTTTGCCCATTGGAATATATTAATTAGTTTCCCCGTACTCTGCGTTGATA
TARGET=207,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5793
PRIMER_LEFT_PENALTY=0.093026
PRIMER_RIGHT_PENALTY=0.486257
PRIMER_LEFT_SEQUENCE=AGTTGAGGTTTGTCGACCATTT
PRIMER_RIGHT_SEQUENCE=CAATGGGCAAAGAAACATGTAG
PRIMER_LEFT=37,22
PRIMER_RIGHT=252,22
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=59.514
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=216
PRIMER_PAIR_PENALTY_1=0.7031
PRIMER_LEFT_1_PENALTY=0.216800
PRIMER_RIGHT_1_PENALTY=0.486257
PRIMER_LEFT_1_SEQUENCE=TCTCGTTTTGACCCCAATAAAC
PRIMER_RIGHT_1_SEQUENCE=CAATGGGCAAAGAAACATGTAG
PRIMER_LEFT_1=136,22
PRIMER_RIGHT_1=252,22
PRIMER_LEFT_1_TM=60.217
PRIMER_RIGHT_1_TM=59.514
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.8226
PRIMER_LEFT_2_PENALTY=0.336392
PRIMER_RIGHT_2_PENALTY=0.486257
PRIMER_LEFT_2_SEQUENCE=CTCTCGTTTTGACCCCAATAAA
PRIMER_RIGHT_2_SEQUENCE=CAATGGGCAAAGAAACATGTAG
PRIMER_LEFT_2=135,22
PRIMER_RIGHT_2=252,22
PRIMER_LEFT_2_TM=60.336
PRIMER_RIGHT_2_TM=59.514
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.2000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=0.8498
PRIMER_LEFT_3_PENALTY=0.363508
PRIMER_RIGHT_3_PENALTY=0.486257
PRIMER_LEFT_3_SEQUENCE=GGGTTTAACGAATGACCTGCTA
PRIMER_RIGHT_3_SEQUENCE=CAATGGGCAAAGAAACATGTAG
PRIMER_LEFT_3=10,22
PRIMER_RIGHT_3=252,22
PRIMER_LEFT_3_TM=60.364
PRIMER_RIGHT_3_TM=59.514
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.9607
PRIMER_LEFT_4_PENALTY=0.474473
PRIMER_RIGHT_4_PENALTY=0.486257
PRIMER_LEFT_4_SEQUENCE=CTCTCTCGTTTTGACCCCAATA
PRIMER_RIGHT_4_SEQUENCE=CAATGGGCAAAGAAACATGTAG
PRIMER_LEFT_4=133,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=60.474
PRIMER_RIGHT_4_TM=59.514
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.2000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=120
=
PRIMER_SEQUENCE_ID=SSR_06038 Ps_contig_mira-and-tgicl-ass_franssen_41278
SEQUENCE=AGGTGAATCATGTTTGACTGCATGATTTTGTGATGATGATGATGATTTTTTATTGGTCTTCAAGTAATTGGTCCTCTTTGGATGGTAAATTATGTTGCTTACACCAAATATCCCAACCAGTTATTGCATCTTCAAGATATCCACTAGATGTATCTGATGCAAACCAATACTCATCAAGAGTAAAATCTGAATGAGTAACTCCAATATCAAGTTCCATGGAGAAACTTAAGGAAGTGAAAACACAAGCAATTGTTATATGAATATTTCTCCCCGAC
TARGET=31,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1379
PRIMER_LEFT_PENALTY=0.134318
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=GGTGAATCATGTTTGACTGCAT
PRIMER_RIGHT_SEQUENCE=TGAAGATGCAATAACTGGTTGG
PRIMER_LEFT=1,22
PRIMER_RIGHT=133,22
PRIMER_LEFT_TM=59.866
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=133
PRIMER_PAIR_PENALTY_1=0.4035
PRIMER_LEFT_1_PENALTY=0.134318
PRIMER_RIGHT_1_PENALTY=0.269153
PRIMER_LEFT_1_SEQUENCE=GGTGAATCATGTTTGACTGCAT
PRIMER_RIGHT_1_SEQUENCE=AGATGCAATAACTGGTTGGGAT
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=130,22
PRIMER_LEFT_1_TM=59.866
PRIMER_RIGHT_1_TM=59.731
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.4000
PRIMER_RIGHT_1_END_STABILITY=9.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=0.5202
PRIMER_LEFT_2_PENALTY=0.134318
PRIMER_RIGHT_2_PENALTY=0.385922
PRIMER_LEFT_2_SEQUENCE=GGTGAATCATGTTTGACTGCAT
PRIMER_RIGHT_2_SEQUENCE=GGTTGGGATATTTGGTGTAAGC
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=117,22
PRIMER_LEFT_2_TM=59.866
PRIMER_RIGHT_2_TM=59.614
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=117
PRIMER_PAIR_PENALTY_3=0.6301
PRIMER_LEFT_3_PENALTY=0.134318
PRIMER_RIGHT_3_PENALTY=0.495821
PRIMER_LEFT_3_SEQUENCE=GGTGAATCATGTTTGACTGCAT
PRIMER_RIGHT_3_SEQUENCE=AACTGGTTGGGATATTTGGTGT
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=121,22
PRIMER_LEFT_3_TM=59.866
PRIMER_RIGHT_3_TM=59.504
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=121
PRIMER_PAIR_PENALTY_4=0.8558
PRIMER_LEFT_4_PENALTY=0.134318
PRIMER_RIGHT_4_PENALTY=0.721451
PRIMER_LEFT_4_SEQUENCE=GGTGAATCATGTTTGACTGCAT
PRIMER_RIGHT_4_SEQUENCE=AAGATGCAATAACTGGTTGGGA
PRIMER_LEFT_4=1,22
PRIMER_RIGHT_4=131,22
PRIMER_LEFT_4_TM=59.866
PRIMER_RIGHT_4_TM=60.721
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.4000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=131
=
PRIMER_SEQUENCE_ID=SSR_06039 Ps_contig_mira-and-tgicl-ass_franssen_41284
SEQUENCE=AAAGACTCTCATGTTTCAACAAACAAACAAGCCTCCATACTGCATCCGANGACAAAAACCTACTATACAGATAGAAGCCCATCAGATGACTTAAAAAATTTGACAAATTGTCAGCAGGTAGGTTTGCTGTTCCTAAAGTTGGCGATACAGTGCATGTCTCTTCTCTTGGGAAAAAAGTGACCATTTTAAAAGTAGATTCATCCAAAGGAGAAGTTGAAGTTCAAGTGGAATCATGAAGTTGAAGCTAAAAGTAACTGACATTCAAAGATTGTAGAAATTAGTAAGTTGTAATCTGTTGATTATTATAGCAGAATCAAACAAATTTCTGCTGTTTACAGCTTATTAAGACGGGATGTAAATAATTAGTTATAGTTCTTTATTCTTTATTGTATTTAGGATTAACATTCATAAGGAGGAATAATTAGNNCTNTGATT
TARGET=18,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06040 Ps_contig_mira-and-tgicl-ass_franssen_41286
SEQUENCE=AGCAACAATCATATGAAGTCCACCTTTCCAGCTACCATTATCGCCGCCGCTACTAAAACTACTACTACTGTTGATGCTTGTTTCYTCTCCTTGGATTGAAGTCTCGTTCGCCGCCAATCCTGGTAGCCATTTAGTTGCATAATGCATTATCCCAGCGCCGATCAGTTCGAATCTCATACCTTTCACCTTAATAGCAGTAATGACTCTGACAAAGTGATCAATCCTAAGGATCGAAACATCTTCAAACCACCAATCAGGAGGAACCAGTTGGTTCCTACTCGGGCTCGAGTCTTTCATTTCGTTCCAACTCGGGCTTGAAACTTTCTGTGCCCTTCCGGTATACGACCACCTTATTCCTTTTGGATTAGCACAAGCTTTCCAAGCGATAGACTCGCTGCATCTTCGAACGATTTGAAGATTTTCTGCCCACGGTGACAACTTCTCCGAGTTCTTCAACACGATGATAGAGTCTCTCCACGAAGACAAAACAACATAG
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06041 Ps_contig_mira-and-tgicl-ass_franssen_41311
SEQUENCE=TTCAACTACTTCNATCTTCCCTCTCCATAACAAAACTCAAAAGAAAGAAAAATAGTACAATCYTTCTCTCTTTTCCTTAACCTTTTATTTCATCTACTTTTTCTTTTCTCTTTTCTCTCCCTAATGGTTCCACCAAATAACAACAACACAACAAAATCATGAAAACCAACAAGATTCTTCTTCAGAATCCAAGAAACCAGATCAACAACAACAACAACAACAAAGAGTTGTAGTAAAATGTCCAAGATGTGACTCTCCCAACACAAAATTCTGTTACTACAACAACTACAGTCTCACTCAACCAAGACACTTCTGCAAAACTTGTAGAAGATATTGGACTAATGGTGGATCCTTGAGGAATGTTCCAATTGGTGGTGGTTGTAGAAAGAAACAAAAACTACAAAAACTCATCTTTAACATCCTCATCCTCATTGTCATCCATTACTAACTTCTCTTCTTCACCTTCACTTGATTTTCATCTTGGTGGCTTATTACCCTTACCTTTACCATTACCCTTCTCTTCAAAACCATATTTCTATAACCAATTCCCTTCAAATTCAAACACTTGTGTTTCTACTACAACTGCTTCAAGTTTTCATCTTGATCCATCTAGGACCTCAAATCCAATGATGGGTCTAAACTTATACCCTTTTAATTCTTCTACTTCAAATGGAAGTGGATTCATCAATGAAAACCCAATTCAAGGAATGAACTTTATGAATGTAAACACTAGTACTACTACTACAA
TARGET=203,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06042 Ps_contig_mira-and-tgicl-ass_franssen_41311
SEQUENCE=TTCAACTACTTCNATCTTCCCTCTCCATAACAAAACTCAAAAGAAAGAAAAATAGTACAATCYTTCTCTCTTTTCCTTAACCTTTTATTTCATCTACTTTTTCTTTTCTCTTTTCTCTCCCTAATGGTTCCACCAAATAACAACAACACAACAAAATCATGAAAACCAACAAGATTCTTCTTCAGAATCCAAGAAACCAGATCAACAACAACAACAACAACAAAGAGTTGTAGTAAAATGTCCAAGATGTGACTCTCCCAACACAAAATTCTGTTACTACAACAACTACAGTCTCACTCAACCAAGACACTTCTGCAAAACTTGTAGAAGATATTGGACTAATGGTGGATCCTTGAGGAATGTTCCAATTGGTGGTGGTTGTAGAAAGAAACAAAAACTACAAAAACTCATCTTTAACATCCTCATCCTCATTGTCATCCATTACTAACTTCTCTTCTTCACCTTCACTTGATTTTCATCTTGGTGGCTTATTACCCTTACCTTTACCATTACCCTTCTCTTCAAAACCATATTTCTATAACCAATTCCCTTCAAATTCAAACACTTGTGTTTCTACTACAACTGCTTCAAGTTTTCATCTTGATCCATCTAGGACCTCAAATCCAATGATGGGTCTAAACTTATACCCTTTTAATTCTTCTACTTCAAATGGAAGTGGATTCATCAATGAAAACCCAATTCAAGGAATGAACTTTATGAATGTAAACACTAGTACTACTACTACAA
TARGET=734,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06043 Ps_contig_mira-and-tgicl-ass_franssen_41332
SEQUENCE=TACTTAATTTCCTAGCTCTTATTCAGTGATCCANGAATAGGAAGTAGTTGGCACTTCCAACGTTAAAGACACATCATAGTCCAAAACAATTGCTGCAGACATCCCACTTGCGAACTCCGACGAGCTTTGCATGAAGTGCCTCTTGCACATCTCTTCTGTTTAAGTAGTTTGTAACCTTGTCATCCACACACACATCTACCATCTCATTTGCTTGTTGAGGTTGAGGAGAAATAACCTTAGATTGTGACAGCACAGAGGAAATACAAACATCAAGAGTCACATCATATTTATCCACAAATCTACTAGTTTCTTTGCTCACCAATCCCATAACATTTGAACAAACAGATGAAACTGAATCTCTATTGTACTCACTAACATAGCGCGAGTAATTACACACTCTAGTGAACATGTTGTAAGTTGAATCAGATATCAGTCCATGTGACCAAAAAAATTCAGCCCTTGAATTGAAATCAGTTGCATATTCCAGAACTGGATTCCCCAAAGCTATGCCTTTGAGATTGAATACATTGTTTCTTTTGTTCCTTTCAATCATAAGCTTTGCAAGTTGAGGAACATAATGACCTGCATAACTTTCACCAGTTAGAAACAAATCTCTGTTTCTATATTGAGGAAACTTATCAAACCAGCGTTCCAAGAATACAAGATTGTCCCTAGCTGTTTCCTCATCATTCACTGTTGTTGTATATGCAGAACTACCTTTTGCATAAGAGAATCCAACTCCAATTGGTGTCTCCAAATACAGCATATTTGCCTCTTTGTTCCAACTATGTTCATTTTTGACAAGAAATTCACCATTTGGTCTGAAGGGTCCATTTTCTGAGAATGCACCAACTCCRAGAGAAGAACAACCAGGTACCAAACCAAATTAAGAGCCAAAGAACAAGAGGCCTTGAAGAAGGNCC
TARGET=185,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06044 Ps_contig_mira-and-tgicl-ass_franssen_41338
SEQUENCE=AAACACAACACAGCTCACTCTTTCTTTCTCTTCCACTCACTTCACTTCACTNCACTTCACTCATCTACCTCATCACAACATCAACAACAAACACACATAACAGAAAAACAACACAATCCAGATCCAAAACATCATTCACAACTTCAACAAGGAAAAAACTACATAAACATAACCGTTAATTTATAACTATAATGCCTTCACTTCCCCAAATTACCCTCACTTTTATGTTCTATTTACTTATCTTATCCCCAACACCATCCTTTTCACTCAACATCACCGCTCTTCTCTCCACTTTCCCTGACTTATCCCAATTCACCGCCCTTCTCTCCACCGCAACTCCCCTCACCGCCGATCTCTCCCACCGCACTTCCATCTCTCTCCTCGCCGTCCCTAACTCCTTCCTCTCCACCGACCCTAACCTCTCCCACCACCAACTCCCCCCCTCCGCACTCACCGACGTCCTGCGCTACCACGTCCTCCTCCAATTCCTCTCATGGTCCGACCTCCACTCCCTCCCTCCTTCCGGCAAACTCATCACCACTCTCTTCCAAACAACCGGCCGCGCCACCAACAATTTCGGCTTTGTCAACATCACTCACGACCCTCACTCCAACCTCGTTTCGATCCGTTCTCCCGCTCCTTATTCCTCCTCAAACGCCACCGTCATTGAATTAGTCAAAACCCTACCGTACAACATTACGATCTTCGCCGTTAATTCTCTCTTAATCCCTTACGGTTTCGATCTCATGGCGTCGGAAACTCGTCCCAGCATGCATCTCAACATCACGAAAACGTTAATCGATGCTCACAACTTCAATGTCGCTGCTTCCATGCTTTCAGCTTCCGGTGTAGTTAACGAATTCGAAGCCGGTGAAGGTGGTTCTGGAATCACATTGTTTATCCCCGTCGATGATGCTTTTGCCGATCTACCTCCTTCAGTTTCGCTTCAGTCACTTCCGGCGGATAAAAAAGCGGTTGTGCTCAAAGCTCATGTTCTCCGTGCTTATTACCCTCTCGGCTCACTTCAATCCACCGCGAATCCCTTGCAGCCAACGCTCGCCACAGAAGCCATGGGAGCTGGAAGCTTCACTCTCAACATTTCAACTTTTAACGGCTCAGTCGCCATTAACACCGGAATTGTTCAGGGGATAATTACTCAGACAGTTTTTGATCAGAACCCTATCGCAATTTTCGGTGTTTCAAAGGTTTTATTACCAAGAGAGATTTTCGGGAAAAATCCAATTGTGTCTGTAAAGTCGCCTCCAGACATTATCGCTCCTCCTCCTTATGACGATGCTTCTTCACCGTCTGGATTTGACGGGCAGCCATCACATCTTTCTTCACCACCTGGGCTTGGCGAAGATGTTAGCTCGAATGTCGCAGGTGTTCATGGTTCTGAGTTATTTATTTTTCTTCTTTGTATAAATTTGTATTTAATGGTATAAAAAGTAATTGTTTTTCTTTTCCTTTGTATTATTATTTTTTTTGTGGGGTTTTGTTTTGATTTCCTCCACCTTTTGTACACGGTTTACTGCATTGTTATTATTATTAGGTTGCAAATGCAGCCTCTTT
TARGET=37,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.7172
PRIMER_LEFT_PENALTY=1.708969
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=GCTCACTCTTTCTTTCTCTTCCA
PRIMER_RIGHT_SEQUENCE=TTGAGTGAAAAGGATGGTGTTG
PRIMER_LEFT=12,23
PRIMER_RIGHT=270,22
PRIMER_LEFT_TM=59.291
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=43.478
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=1.7382
PRIMER_LEFT_1_PENALTY=1.708969
PRIMER_RIGHT_1_PENALTY=0.029253
PRIMER_LEFT_1_SEQUENCE=GCTCACTCTTTCTTTCTCTTCCA
PRIMER_RIGHT_1_SEQUENCE=TGATGTTGAGTGAAAAGGATGG
PRIMER_LEFT_1=12,23
PRIMER_RIGHT_1=275,22
PRIMER_LEFT_1_TM=59.291
PRIMER_RIGHT_1_TM=59.971
PRIMER_LEFT_1_GC_PERCENT=43.478
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=264
PRIMER_PAIR_PENALTY_2=1.7995
PRIMER_LEFT_2_PENALTY=1.708969
PRIMER_RIGHT_2_PENALTY=0.090580
PRIMER_LEFT_2_SEQUENCE=GCTCACTCTTTCTTTCTCTTCCA
PRIMER_RIGHT_2_SEQUENCE=GGATGGTGTTGGGGATAAGATA
PRIMER_LEFT_2=12,23
PRIMER_RIGHT_2=259,22
PRIMER_LEFT_2_TM=59.291
PRIMER_RIGHT_2_TM=59.909
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=1.8177
PRIMER_LEFT_3_PENALTY=1.708969
PRIMER_RIGHT_3_PENALTY=0.108707
PRIMER_LEFT_3_SEQUENCE=GCTCACTCTTTCTTTCTCTTCCA
PRIMER_RIGHT_3_SEQUENCE=AGTGAGGGTAATTTGGGGAAGT
PRIMER_LEFT_3=12,23
PRIMER_RIGHT_3=220,22
PRIMER_LEFT_3_TM=59.291
PRIMER_RIGHT_3_TM=60.109
PRIMER_LEFT_3_GC_PERCENT=43.478
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=2.0495
PRIMER_LEFT_4_PENALTY=2.020269
PRIMER_RIGHT_4_PENALTY=0.029253
PRIMER_LEFT_4_SEQUENCE=CACAACACAGCTCACTCTTTCTTT
PRIMER_RIGHT_4_SEQUENCE=TGATGTTGAGTGAAAAGGATGG
PRIMER_LEFT_4=3,24
PRIMER_RIGHT_4=275,22
PRIMER_LEFT_4_TM=60.020
PRIMER_RIGHT_4_TM=59.971
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_06045 Ps_contig_mira-and-tgicl-ass_franssen_41338
SEQUENCE=AAACACAACACAGCTCACTCTTTCTTTCTCTTCCACTCACTTCACTTCACTNCACTTCACTCATCTACCTCATCACAACATCAACAACAAACACACATAACAGAAAAACAACACAATCCAGATCCAAAACATCATTCACAACTTCAACAAGGAAAAAACTACATAAACATAACCGTTAATTTATAACTATAATGCCTTCACTTCCCCAAATTACCCTCACTTTTATGTTCTATTTACTTATCTTATCCCCAACACCATCCTTTTCACTCAACATCACCGCTCTTCTCTCCACTTTCCCTGACTTATCCCAATTCACCGCCCTTCTCTCCACCGCAACTCCCCTCACCGCCGATCTCTCCCACCGCACTTCCATCTCTCTCCTCGCCGTCCCTAACTCCTTCCTCTCCACCGACCCTAACCTCTCCCACCACCAACTCCCCCCCTCCGCACTCACCGACGTCCTGCGCTACCACGTCCTCCTCCAATTCCTCTCATGGTCCGACCTCCACTCCCTCCCTCCTTCCGGCAAACTCATCACCACTCTCTTCCAAACAACCGGCCGCGCCACCAACAATTTCGGCTTTGTCAACATCACTCACGACCCTCACTCCAACCTCGTTTCGATCCGTTCTCCCGCTCCTTATTCCTCCTCAAACGCCACCGTCATTGAATTAGTCAAAACCCTACCGTACAACATTACGATCTTCGCCGTTAATTCTCTCTTAATCCCTTACGGTTTCGATCTCATGGCGTCGGAAACTCGTCCCAGCATGCATCTCAACATCACGAAAACGTTAATCGATGCTCACAACTTCAATGTCGCTGCTTCCATGCTTTCAGCTTCCGGTGTAGTTAACGAATTCGAAGCCGGTGAAGGTGGTTCTGGAATCACATTGTTTATCCCCGTCGATGATGCTTTTGCCGATCTACCTCCTTCAGTTTCGCTTCAGTCACTTCCGGCGGATAAAAAAGCGGTTGTGCTCAAAGCTCATGTTCTCCGTGCTTATTACCCTCTCGGCTCACTTCAATCCACCGCGAATCCCTTGCAGCCAACGCTCGCCACAGAAGCCATGGGAGCTGGAAGCTTCACTCTCAACATTTCAACTTTTAACGGCTCAGTCGCCATTAACACCGGAATTGTTCAGGGGATAATTACTCAGACAGTTTTTGATCAGAACCCTATCGCAATTTTCGGTGTTTCAAAGGTTTTATTACCAAGAGAGATTTTCGGGAAAAATCCAATTGTGTCTGTAAAGTCGCCTCCAGACATTATCGCTCCTCCTCCTTATGACGATGCTTCTTCACCGTCTGGATTTGACGGGCAGCCATCACATCTTTCTTCACCACCTGGGCTTGGCGAAGATGTTAGCTCGAATGTCGCAGGTGTTCATGGTTCTGAGTTATTTATTTTTCTTCTTTGTATAAATTTGTATTTAATGGTATAAAAAGTAATTGTTTTTCTTTTCCTTTGTATTATTATTTTTTTTGTGGGGTTTTGTTTTGATTTCCTCCACCTTTTGTACACGGTTTACTGCATTGTTATTATTATTAGGTTGCAAATGCAGCCTCTTT
TARGET=509,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1609
PRIMER_LEFT_PENALTY=0.012411
PRIMER_RIGHT_PENALTY=0.148509
PRIMER_LEFT_SEQUENCE=CTCTTCTCTCCACTTTCCCTGA
PRIMER_RIGHT_SEQUENCE=TGTTTGGAAGAGAGTGGTGATG
PRIMER_LEFT=279,22
PRIMER_RIGHT=553,22
PRIMER_LEFT_TM=59.988
PRIMER_RIGHT_TM=60.149
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.3355
PRIMER_LEFT_1_PENALTY=0.186949
PRIMER_RIGHT_1_PENALTY=0.148509
PRIMER_LEFT_1_SEQUENCE=TCCCTGACTTATCCCAATTCAC
PRIMER_RIGHT_1_SEQUENCE=TGTTTGGAAGAGAGTGGTGATG
PRIMER_LEFT_1=294,22
PRIMER_RIGHT_1=553,22
PRIMER_LEFT_1_TM=60.187
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=260
PRIMER_PAIR_PENALTY_2=0.4930
PRIMER_LEFT_2_PENALTY=0.344527
PRIMER_RIGHT_2_PENALTY=0.148509
PRIMER_LEFT_2_SEQUENCE=CACTTTCCCTGACTTATCCCAA
PRIMER_RIGHT_2_SEQUENCE=TGTTTGGAAGAGAGTGGTGATG
PRIMER_LEFT_2=289,22
PRIMER_RIGHT_2=553,22
PRIMER_LEFT_2_TM=60.345
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=265
PRIMER_PAIR_PENALTY_3=0.6986
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.690327
PRIMER_LEFT_3_SEQUENCE=CAACACCATCCTTTTCACTCAA
PRIMER_RIGHT_3_SEQUENCE=AGAGAGTGGTGATGAGTTTGCC
PRIMER_LEFT_3=249,22
PRIMER_RIGHT_3=545,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=60.690
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=297
PRIMER_PAIR_PENALTY_4=0.7027
PRIMER_LEFT_4_PENALTY=0.012411
PRIMER_RIGHT_4_PENALTY=0.690327
PRIMER_LEFT_4_SEQUENCE=CTCTTCTCTCCACTTTCCCTGA
PRIMER_RIGHT_4_SEQUENCE=AGAGAGTGGTGATGAGTTTGCC
PRIMER_LEFT_4=279,22
PRIMER_RIGHT_4=545,22
PRIMER_LEFT_4_TM=59.988
PRIMER_RIGHT_4_TM=60.690
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_06046 Ps_contig_mira-and-tgicl-ass_franssen_41338
SEQUENCE=AAACACAACACAGCTCACTCTTTCTTTCTCTTCCACTCACTTCACTTCACTNCACTTCACTCATCTACCTCATCACAACATCAACAACAAACACACATAACAGAAAAACAACACAATCCAGATCCAAAACATCATTCACAACTTCAACAAGGAAAAAACTACATAAACATAACCGTTAATTTATAACTATAATGCCTTCACTTCCCCAAATTACCCTCACTTTTATGTTCTATTTACTTATCTTATCCCCAACACCATCCTTTTCACTCAACATCACCGCTCTTCTCTCCACTTTCCCTGACTTATCCCAATTCACCGCCCTTCTCTCCACCGCAACTCCCCTCACCGCCGATCTCTCCCACCGCACTTCCATCTCTCTCCTCGCCGTCCCTAACTCCTTCCTCTCCACCGACCCTAACCTCTCCCACCACCAACTCCCCCCCTCCGCACTCACCGACGTCCTGCGCTACCACGTCCTCCTCCAATTCCTCTCATGGTCCGACCTCCACTCCCTCCCTCCTTCCGGCAAACTCATCACCACTCTCTTCCAAACAACCGGCCGCGCCACCAACAATTTCGGCTTTGTCAACATCACTCACGACCCTCACTCCAACCTCGTTTCGATCCGTTCTCCCGCTCCTTATTCCTCCTCAAACGCCACCGTCATTGAATTAGTCAAAACCCTACCGTACAACATTACGATCTTCGCCGTTAATTCTCTCTTAATCCCTTACGGTTTCGATCTCATGGCGTCGGAAACTCGTCCCAGCATGCATCTCAACATCACGAAAACGTTAATCGATGCTCACAACTTCAATGTCGCTGCTTCCATGCTTTCAGCTTCCGGTGTAGTTAACGAATTCGAAGCCGGTGAAGGTGGTTCTGGAATCACATTGTTTATCCCCGTCGATGATGCTTTTGCCGATCTACCTCCTTCAGTTTCGCTTCAGTCACTTCCGGCGGATAAAAAAGCGGTTGTGCTCAAAGCTCATGTTCTCCGTGCTTATTACCCTCTCGGCTCACTTCAATCCACCGCGAATCCCTTGCAGCCAACGCTCGCCACAGAAGCCATGGGAGCTGGAAGCTTCACTCTCAACATTTCAACTTTTAACGGCTCAGTCGCCATTAACACCGGAATTGTTCAGGGGATAATTACTCAGACAGTTTTTGATCAGAACCCTATCGCAATTTTCGGTGTTTCAAAGGTTTTATTACCAAGAGAGATTTTCGGGAAAAATCCAATTGTGTCTGTAAAGTCGCCTCCAGACATTATCGCTCCTCCTCCTTATGACGATGCTTCTTCACCGTCTGGATTTGACGGGCAGCCATCACATCTTTCTTCACCACCTGGGCTTGGCGAAGATGTTAGCTCGAATGTCGCAGGTGTTCATGGTTCTGAGTTATTTATTTTTCTTCTTTGTATAAATTTGTATTTAATGGTATAAAAAGTAATTGTTTTTCTTTTCCTTTGTATTATTATTTTTTTTGTGGGGTTTTGTTTTGATTTCCTCCACCTTTTGTACACGGTTTACTGCATTGTTATTATTATTAGGTTGCAAATGCAGCCTCTTT
TARGET=1540,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06047 Ps_contig_mira-and-tgicl-ass_franssen_41351
SEQUENCE=CCGTGCCGTGCCGTGCCGTGCCGTGCCGGAGGTCCTCCCCGGCGGCGGAGGCGCCACAAAATCAAATCAAACAACAATATCTAAATCAATTCCACCTCCTGACACTGAATCTACAATTGGATTGTCCTAATTCTAACAAACCAGTCCGAATTGAAACTTACAACCGAAGAACCCTAATTTGAAGAATAAAAAATTAAATGGTGCATGTTGGGAATCAAAGCCCCCGGGGTCAGGGATTTGGTTTCCCTAACCTCTAATCACATCATGGACTCACCGATGAAGCGCGCGCAGTGGTTA
TARGET=1,25
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06048 Ps_contig_mira-and-tgicl-ass_franssen_41356
SEQUENCE=CGTTGTGGTTGACTTTTAGAGCGTAGATGGTAGAGCTGACTTTGTGGCGGACTTTGTAGACGGTGCCTCCGTTGCCATGACCGAGGACGGCGAGTTTCTCGAAGTCACCCGGAGAAAGGGTGTCACTGTCTCTAGGCTTCCTATTTTACCAGAATAATTCAAAGTTTTCTCCCATTCTAACATATTTCTCTCCATTGGTTTGTACATGTATGTATGTAATAACATGTACAGTCATGCAAG
TARGET=206,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06049 Ps_contig_mira-and-tgicl-ass_franssen_41366
SEQUENCE=GTCTTTGGTTCGTTTTGTAGGAAATAGGGTTCCCTAACGGGCCGGGTGGGGTTTCCCGTTCACAATGGTCTAGCTAGGAAGGCAGGGAAAGGAATTGTGAATATTGGCAGNGTTACTGGATTGGCCTCTGGACCTTGGCGGGTGCTTACAGTGCTTCTAAGGCTGCCATTCATGCTTTGACAGATACATTAAGATTGGAACTTGGTCATTTTGGAATTGATGTTGTGAACATTGTCCCTAGGAGCTGTGAAGTCGAATATTGGAAATTCTGGCATATCCATCTACAACAGCATGCCAAAGTGGAAACTTGTTTAAGCCTATTTGAAGCAGCAATCAGAGAGAGAGCTCTTACTTTCNCAGTAGGTTACAAAGACTACCCAACCCCTANAGATGTAGTTNACTAAACATAC
TARGET=336,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9495
PRIMER_LEFT_PENALTY=0.011696
PRIMER_RIGHT_PENALTY=0.937844
PRIMER_LEFT_SEQUENCE=GGCATATCCATCTACAACAGCA
PRIMER_RIGHT_SEQUENCE=TNTAGGGGTTGGGTAGTCTTTG
PRIMER_LEFT=270,22
PRIMER_RIGHT=388,22
PRIMER_LEFT_TM=59.988
PRIMER_RIGHT_TM=59.062
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=7.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.9495
PRIMER_LEFT_1_PENALTY=0.011696
PRIMER_RIGHT_1_PENALTY=0.937844
PRIMER_LEFT_1_SEQUENCE=TGGCATATCCATCTACAACAGC
PRIMER_RIGHT_1_SEQUENCE=TNTAGGGGTTGGGTAGTCTTTG
PRIMER_LEFT_1=269,22
PRIMER_RIGHT_1=388,22
PRIMER_LEFT_1_TM=59.988
PRIMER_RIGHT_1_TM=59.062
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=1.0406
PRIMER_LEFT_2_PENALTY=0.102789
PRIMER_RIGHT_2_PENALTY=0.937844
PRIMER_LEFT_2_SEQUENCE=GCCATTCATGCTTTGACAGATA
PRIMER_RIGHT_2_SEQUENCE=TNTAGGGGTTGGGTAGTCTTTG
PRIMER_LEFT_2=164,22
PRIMER_RIGHT_2=388,22
PRIMER_LEFT_2_TM=60.103
PRIMER_RIGHT_2_TM=59.062
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=7.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=1.0653
PRIMER_LEFT_3_PENALTY=0.011696
PRIMER_RIGHT_3_PENALTY=1.053582
PRIMER_LEFT_3_SEQUENCE=GGCATATCCATCTACAACAGCA
PRIMER_RIGHT_3_SEQUENCE=CTNTAGGGGTTGGGTAGTCTTTG
PRIMER_LEFT_3=270,22
PRIMER_RIGHT_3=389,23
PRIMER_LEFT_3_TM=59.988
PRIMER_RIGHT_3_TM=59.946
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.50
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=4.75
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=1.0653
PRIMER_LEFT_4_PENALTY=0.011696
PRIMER_RIGHT_4_PENALTY=1.053582
PRIMER_LEFT_4_SEQUENCE=TGGCATATCCATCTACAACAGC
PRIMER_RIGHT_4_SEQUENCE=CTNTAGGGGTTGGGTAGTCTTTG
PRIMER_LEFT_4=269,22
PRIMER_RIGHT_4=389,23
PRIMER_LEFT_4_TM=59.988
PRIMER_RIGHT_4_TM=59.946
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.50
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.75
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_06050 Ps_contig_mira-and-tgicl-ass_franssen_41403
SEQUENCE=AGTNTAAAAATAAAGTGTTATGTGTTTAATATATAATAAACATTAGCAATATTTTTCTTTGCGCTACATATGACACTAAACACACTGCAAATTTTTACACCCAATATCTTTTTGAAAAAACGATAACAAATCACACTTATTTCAGACACTACTAGTATTATATTCGAGATGAACACAAAGACTATCAACACACTTGTCTCAATAACAACTACATATACATATGATTATAAGTCTTCACTCACTTCAACGACTCATGCCCAATTCGACCGTTGGAGCAGTAGTCGTAGCTGCAGCTGAAGCCATCACTATTCCTCGAGTCCGTTGCTCCACATTTCCATGCCACCCGATAACCATGTTAAATCCACGGTGTTCGAGCTCACGATATTCTTGACACAAGGCGCAAGCCTCACAGAAACAATGAATGATACAATCCGTACAATCATTTCCTTTCAAACCATATTGTTGTCTCATCTTTGAACGGTAGAAGCAAGAATACAAACAACCACACCCAATCACACAACATATCAACGTGTACAATGCCCCACTAGCACCACATGAAGTGGATCCCTTGTCAATGATTTCCGCAACTTGGCCAAAAGTAATACATGGACACCAGAATGTTATGCAACTTTTTCCGGGGTCAGAACAGCAATCACAAAGACCGGTGGACCACTCAACTATAGGCTTGGGTGGAGGAGGAGGCGGCGGCTGGTAGTCAGACGATGTGGTGACGGAGTATGTGGATGCATCGCTGTTGAAGCTGACCGGAAAGCCTGTGGCAGCGGCTGGTTGTGGCTGATTGTAGGATGGCTTTCCTTGCTGTGGTGGATACATTTTTGGAAAGTCAGGTGTTGTCAATATGTGAATGAATGAATTTTGGTCGCATTTATAATTGTGGAAAATTTGTGTGAGTGYGNAATGGTTCAACGTTGGAGGATTCTATAAGCGCGTCTTCTTCTTCTACAACTTGTTCGTGTTCGTTACTTCCACTATTATTATTTTTATCAAACAAAAGAAAAAC
TARGET=863,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06051 Ps_contig_mira-and-tgicl-ass_franssen_41403
SEQUENCE=AGTNTAAAAATAAAGTGTTATGTGTTTAATATATAATAAACATTAGCAATATTTTTCTTTGCGCTACATATGACACTAAACACACTGCAAATTTTTACACCCAATATCTTTTTGAAAAAACGATAACAAATCACACTTATTTCAGACACTACTAGTATTATATTCGAGATGAACACAAAGACTATCAACACACTTGTCTCAATAACAACTACATATACATATGATTATAAGTCTTCACTCACTTCAACGACTCATGCCCAATTCGACCGTTGGAGCAGTAGTCGTAGCTGCAGCTGAAGCCATCACTATTCCTCGAGTCCGTTGCTCCACATTTCCATGCCACCCGATAACCATGTTAAATCCACGGTGTTCGAGCTCACGATATTCTTGACACAAGGCGCAAGCCTCACAGAAACAATGAATGATACAATCCGTACAATCATTTCCTTTCAAACCATATTGTTGTCTCATCTTTGAACGGTAGAAGCAAGAATACAAACAACCACACCCAATCACACAACATATCAACGTGTACAATGCCCCACTAGCACCACATGAAGTGGATCCCTTGTCAATGATTTCCGCAACTTGGCCAAAAGTAATACATGGACACCAGAATGTTATGCAACTTTTTCCGGGGTCAGAACAGCAATCACAAAGACCGGTGGACCACTCAACTATAGGCTTGGGTGGAGGAGGAGGCGGCGGCTGGTAGTCAGACGATGTGGTGACGGAGTATGTGGATGCATCGCTGTTGAAGCTGACCGGAAAGCCTGTGGCAGCGGCTGGTTGTGGCTGATTGTAGGATGGCTTTCCTTGCTGTGGTGGATACATTTTTGGAAAGTCAGGTGTTGTCAATATGTGAATGAATGAATTTTGGTCGCATTTATAATTGTGGAAAATTTGTGTGAGTGYGNAATGGTTCAACGTTGGAGGATTCTATAAGCGCGTCTTCTTCTTCTACAACTTGTTCGTGTTCGTTACTTCCACTATTATTATTTTTATCAAACAAAAGAAAAAC
TARGET=951,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06052 Ps_contig_mira-and-tgicl-ass_franssen_41428
SEQUENCE=TCACCTCACAACAACACTTCCACTCTCACCTTTCTCCAAAACATTATCATAATAGTTCACCACCACCACCAGTAGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATACTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAA
TARGET=58,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3413
PRIMER_LEFT_PENALTY=0.335679
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=CCTCACAACAACACTTCCACTC
PRIMER_RIGHT_SEQUENCE=GTGATAAGGTGGAGGAGGTGAG
PRIMER_LEFT=3,22
PRIMER_RIGHT=215,22
PRIMER_LEFT_TM=59.664
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.4025
PRIMER_LEFT_1_PENALTY=0.335679
PRIMER_RIGHT_1_PENALTY=0.066849
PRIMER_LEFT_1_SEQUENCE=CCTCACAACAACACTTCCACTC
PRIMER_RIGHT_1_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=250,22
PRIMER_LEFT_1_TM=59.664
PRIMER_RIGHT_1_TM=60.067
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=248
PRIMER_PAIR_PENALTY_2=0.6584
PRIMER_LEFT_2_PENALTY=0.335679
PRIMER_RIGHT_2_PENALTY=0.322707
PRIMER_LEFT_2_SEQUENCE=CAACAACACTTCCACTCTCACC
PRIMER_RIGHT_2_SEQUENCE=GTACGACTTCTTTGGTGGTGGT
PRIMER_LEFT_2=8,22
PRIMER_RIGHT_2=248,22
PRIMER_LEFT_2_TM=59.664
PRIMER_RIGHT_2_TM=60.323
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=0.6584
PRIMER_LEFT_3_PENALTY=0.335679
PRIMER_RIGHT_3_PENALTY=0.322707
PRIMER_LEFT_3_SEQUENCE=CCTCACAACAACACTTCCACTC
PRIMER_RIGHT_3_SEQUENCE=GTACGACTTCTTTGGTGGTGGT
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=248,22
PRIMER_LEFT_3_TM=59.664
PRIMER_RIGHT_3_TM=60.323
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=0.6986
PRIMER_LEFT_4_PENALTY=0.631747
PRIMER_RIGHT_4_PENALTY=0.066849
PRIMER_LEFT_4_SEQUENCE=CCACTCTCACCTTTCTCCAAAA
PRIMER_RIGHT_4_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=250,22
PRIMER_LEFT_4_TM=60.632
PRIMER_RIGHT_4_TM=60.067
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=232
=
PRIMER_SEQUENCE_ID=SSR_06053 Ps_contig_mira-and-tgicl-ass_franssen_41428
SEQUENCE=TCACCTCACAACAACACTTCCACTCTCACCTTTCTCCAAAACATTATCATAATAGTTCACCACCACCACCAGTAGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATACTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAA
TARGET=107,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0724
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.066849
PRIMER_LEFT_SEQUENCE=CACTCACCTCCTCCACCTTATC
PRIMER_RIGHT_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_LEFT=75,22
PRIMER_RIGHT=250,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.067
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.0724
PRIMER_LEFT_1_PENALTY=0.005574
PRIMER_RIGHT_1_PENALTY=0.066849
PRIMER_LEFT_1_SEQUENCE=CTCACCTCCTCCACCTTATCAC
PRIMER_RIGHT_1_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_LEFT_1=77,22
PRIMER_RIGHT_1=250,22
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=60.067
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=174
PRIMER_PAIR_PENALTY_2=0.0724
PRIMER_LEFT_2_PENALTY=0.005574
PRIMER_RIGHT_2_PENALTY=0.066849
PRIMER_LEFT_2_SEQUENCE=TCACCTCCTCCACCTTATCACT
PRIMER_RIGHT_2_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_LEFT_2=78,22
PRIMER_RIGHT_2=250,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.067
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.0724
PRIMER_LEFT_3_PENALTY=0.005574
PRIMER_RIGHT_3_PENALTY=0.066849
PRIMER_LEFT_3_SEQUENCE=ACTCACCTCCTCCACCTTATCA
PRIMER_RIGHT_3_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_LEFT_3=76,22
PRIMER_RIGHT_3=250,22
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=60.067
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.3283
PRIMER_LEFT_4_PENALTY=0.005574
PRIMER_RIGHT_4_PENALTY=0.322707
PRIMER_LEFT_4_SEQUENCE=CTCACCTCCTCCACCTTATCAC
PRIMER_RIGHT_4_SEQUENCE=GTACGACTTCTTTGGTGGTGGT
PRIMER_LEFT_4=77,22
PRIMER_RIGHT_4=248,22
PRIMER_LEFT_4_TM=59.994
PRIMER_RIGHT_4_TM=60.323
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_06054 Ps_contig_mira-and-tgicl-ass_franssen_41428
SEQUENCE=TCACCTCACAACAACACTTCCACTCTCACCTTTCTCCAAAACATTATCATAATAGTTCACCACCACCACCAGTAGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATACTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAA
TARGET=176,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0724
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.066849
PRIMER_LEFT_SEQUENCE=CACTCACCTCCTCCACCTTATC
PRIMER_RIGHT_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_LEFT=75,22
PRIMER_RIGHT=250,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.067
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.0724
PRIMER_LEFT_1_PENALTY=0.005574
PRIMER_RIGHT_1_PENALTY=0.066849
PRIMER_LEFT_1_SEQUENCE=CTCACCTCCTCCACCTTATCAC
PRIMER_RIGHT_1_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_LEFT_1=77,22
PRIMER_RIGHT_1=250,22
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=60.067
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=174
PRIMER_PAIR_PENALTY_2=0.0724
PRIMER_LEFT_2_PENALTY=0.005574
PRIMER_RIGHT_2_PENALTY=0.066849
PRIMER_LEFT_2_SEQUENCE=TCACCTCCTCCACCTTATCACT
PRIMER_RIGHT_2_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_LEFT_2=78,22
PRIMER_RIGHT_2=250,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.067
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=0.0724
PRIMER_LEFT_3_PENALTY=0.005574
PRIMER_RIGHT_3_PENALTY=0.066849
PRIMER_LEFT_3_SEQUENCE=ACTCACCTCCTCCACCTTATCA
PRIMER_RIGHT_3_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_LEFT_3=76,22
PRIMER_RIGHT_3=250,22
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=60.067
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.3283
PRIMER_LEFT_4_PENALTY=0.005574
PRIMER_RIGHT_4_PENALTY=0.322707
PRIMER_LEFT_4_SEQUENCE=CTCACCTCCTCCACCTTATCAC
PRIMER_RIGHT_4_SEQUENCE=GTACGACTTCTTTGGTGGTGGT
PRIMER_LEFT_4=77,22
PRIMER_RIGHT_4=248,22
PRIMER_LEFT_4_TM=59.994
PRIMER_RIGHT_4_TM=60.323
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=172
=
PRIMER_SEQUENCE_ID=SSR_06055 Ps_contig_mira-and-tgicl-ass_franssen_41428
SEQUENCE=TCACCTCACAACAACACTTCCACTCTCACCTTTCTCCAAAACATTATCATAATAGTTCACCACCACCACCAGTAGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATACTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAA
TARGET=224,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0589
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.053348
PRIMER_LEFT_SEQUENCE=CTCACCTCCTCCACCTTATCAC
PRIMER_RIGHT_SEQUENCE=GGTGGTGGTGGAGATTGATATT
PRIMER_LEFT=194,22
PRIMER_RIGHT=460,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=59.947
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=5.8000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.0589
PRIMER_LEFT_1_PENALTY=0.005574
PRIMER_RIGHT_1_PENALTY=0.053348
PRIMER_LEFT_1_SEQUENCE=TCACCTCCTCCACCTTATCACT
PRIMER_RIGHT_1_SEQUENCE=GGTGGTGGTGGAGATTGATATT
PRIMER_LEFT_1=195,22
PRIMER_RIGHT_1=460,22
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=59.947
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=5.8000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.0589
PRIMER_LEFT_2_PENALTY=0.005574
PRIMER_RIGHT_2_PENALTY=0.053348
PRIMER_LEFT_2_SEQUENCE=ACTCACCTCCTCCACCTTATCA
PRIMER_RIGHT_2_SEQUENCE=GGTGGTGGTGGAGATTGATATT
PRIMER_LEFT_2=193,22
PRIMER_RIGHT_2=460,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=59.947
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.3205
PRIMER_LEFT_3_PENALTY=0.005574
PRIMER_RIGHT_3_PENALTY=0.314970
PRIMER_LEFT_3_SEQUENCE=CTCACCTCCTCCACCTTATCAC
PRIMER_RIGHT_3_SEQUENCE=TGGTGGTGGAGATTGATATTTG
PRIMER_LEFT_3=194,22
PRIMER_RIGHT_3=458,22
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=59.685
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=265
PRIMER_PAIR_PENALTY_4=0.3205
PRIMER_LEFT_4_PENALTY=0.005574
PRIMER_RIGHT_4_PENALTY=0.314970
PRIMER_LEFT_4_SEQUENCE=CACTCACCTCCTCCACCTTATC
PRIMER_RIGHT_4_SEQUENCE=TGGTGGTGGAGATTGATATTTG
PRIMER_LEFT_4=192,22
PRIMER_RIGHT_4=458,22
PRIMER_LEFT_4_TM=59.994
PRIMER_RIGHT_4_TM=59.685
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=8.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_06056 Ps_contig_mira-and-tgicl-ass_franssen_41428
SEQUENCE=TCACCTCACAACAACACTTCCACTCTCACCTTTCTCCAAAACATTATCATAATAGTTCACCACCACCACCAGTAGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATACTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAA
TARGET=451,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4617
PRIMER_LEFT_PENALTY=0.456098
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=ACCACCACCGGTGTACAAGTAT
PRIMER_RIGHT_SEQUENCE=GTGATAAGGTGGAGGAGGTGAG
PRIMER_LEFT=362,22
PRIMER_RIGHT=620,22
PRIMER_LEFT_TM=60.456
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.3000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=0.4617
PRIMER_LEFT_1_PENALTY=0.456098
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=ACCACCACCGGTGTACAAGTAT
PRIMER_RIGHT_1_SEQUENCE=GATAAGGTGGAGGAGGTGAGTG
PRIMER_LEFT_1=362,22
PRIMER_RIGHT_1=618,22
PRIMER_LEFT_1_TM=60.456
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=257
PRIMER_PAIR_PENALTY_2=0.4617
PRIMER_LEFT_2_PENALTY=0.456098
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=ACCACCACCGGTGTACAAGTAT
PRIMER_RIGHT_2_SEQUENCE=TGATAAGGTGGAGGAGGTGAGT
PRIMER_LEFT_2=362,22
PRIMER_RIGHT_2=619,22
PRIMER_LEFT_2_TM=60.456
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.4617
PRIMER_LEFT_3_PENALTY=0.456098
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=ACCACCACCGGTGTACAAGTAT
PRIMER_RIGHT_3_SEQUENCE=AGTGATAAGGTGGAGGAGGTGA
PRIMER_LEFT_3=362,22
PRIMER_RIGHT_3=621,22
PRIMER_LEFT_3_TM=60.456
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.3000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.4827
PRIMER_LEFT_4_PENALTY=0.415851
PRIMER_RIGHT_4_PENALTY=0.066849
PRIMER_LEFT_4_SEQUENCE=ACAAATACAAATCTCCTCCCCC
PRIMER_RIGHT_4_SEQUENCE=TTGTACGACTTCTTTGGTGGTG
PRIMER_LEFT_4=406,22
PRIMER_RIGHT_4=658,22
PRIMER_LEFT_4_TM=60.416
PRIMER_RIGHT_4_TM=60.067
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=12.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_06057 Ps_contig_mira-and-tgicl-ass_franssen_41428
SEQUENCE=TCACCTCACAACAACACTTCCACTCTCACCTTTCTCCAAAACATTATCATAATAGTTCACCACCACCACCAGTAGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATACTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAA
TARGET=512,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1151
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=CCACCACCACCTAAGAAATCAT
PRIMER_RIGHT_SEQUENCE=GTGATAAGGTGGAGGAGGTGAG
PRIMER_LEFT=453,22
PRIMER_RIGHT=620,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.1151
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=CCACCACCACCTAAGAAATCAT
PRIMER_RIGHT_1_SEQUENCE=TGATAAGGTGGAGGAGGTGAGT
PRIMER_LEFT_1=453,22
PRIMER_RIGHT_1=619,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.1151
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=CCACCACCACCTAAGAAATCAT
PRIMER_RIGHT_2_SEQUENCE=AGTGATAAGGTGGAGGAGGTGA
PRIMER_LEFT_2=453,22
PRIMER_RIGHT_2=621,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.4617
PRIMER_LEFT_3_PENALTY=0.456098
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=ACCACCACCGGTGTACAAGTAT
PRIMER_RIGHT_3_SEQUENCE=GTGATAAGGTGGAGGAGGTGAG
PRIMER_LEFT_3=362,22
PRIMER_RIGHT_3=620,22
PRIMER_LEFT_3_TM=60.456
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.4617
PRIMER_LEFT_4_PENALTY=0.456098
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=ACCACCACCGGTGTACAAGTAT
PRIMER_RIGHT_4_SEQUENCE=GATAAGGTGGAGGAGGTGAGTG
PRIMER_LEFT_4=362,22
PRIMER_RIGHT_4=618,22
PRIMER_LEFT_4_TM=60.456
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_06058 Ps_contig_mira-and-tgicl-ass_franssen_41428
SEQUENCE=TCACCTCACAACAACACTTCCACTCTCACCTTTCTCCAAAACATTATCATAATAGTTCACCACCACCACCAGTAGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATACTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAA
TARGET=581,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1151
PRIMER_LEFT_PENALTY=0.109510
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=CCACCACCACCTAAGAAATCAT
PRIMER_RIGHT_SEQUENCE=GTGATAAGGTGGAGGAGGTGAG
PRIMER_LEFT=453,22
PRIMER_RIGHT=620,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.5000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.1151
PRIMER_LEFT_1_PENALTY=0.109510
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=CCACCACCACCTAAGAAATCAT
PRIMER_RIGHT_1_SEQUENCE=TGATAAGGTGGAGGAGGTGAGT
PRIMER_LEFT_1=453,22
PRIMER_RIGHT_1=619,22
PRIMER_LEFT_1_TM=60.110
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=167
PRIMER_PAIR_PENALTY_2=0.1151
PRIMER_LEFT_2_PENALTY=0.109510
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=CCACCACCACCTAAGAAATCAT
PRIMER_RIGHT_2_SEQUENCE=AGTGATAAGGTGGAGGAGGTGA
PRIMER_LEFT_2=453,22
PRIMER_RIGHT_2=621,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.5000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=169
PRIMER_PAIR_PENALTY_3=0.4617
PRIMER_LEFT_3_PENALTY=0.456098
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=ACCACCACCGGTGTACAAGTAT
PRIMER_RIGHT_3_SEQUENCE=GTGATAAGGTGGAGGAGGTGAG
PRIMER_LEFT_3=362,22
PRIMER_RIGHT_3=620,22
PRIMER_LEFT_3_TM=60.456
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.4617
PRIMER_LEFT_4_PENALTY=0.456098
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=ACCACCACCGGTGTACAAGTAT
PRIMER_RIGHT_4_SEQUENCE=GATAAGGTGGAGGAGGTGAGTG
PRIMER_LEFT_4=362,22
PRIMER_RIGHT_4=618,22
PRIMER_LEFT_4_TM=60.456
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_06059 Ps_contig_mira-and-tgicl-ass_franssen_41428
SEQUENCE=TCACCTCACAACAACACTTCCACTCTCACCTTTCTCCAAAACATTATCATAATAGTTCACCACCACCACCAGTAGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATACTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAA
TARGET=629,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5717
PRIMER_LEFT_PENALTY=0.566078
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=CACCACCGGTGTACAAATACAA
PRIMER_RIGHT_SEQUENCE=TGGTGGTGGAGGAGAAGAGTAT
PRIMER_LEFT=394,22
PRIMER_RIGHT=680,22
PRIMER_LEFT_TM=60.566
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=5.3000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.8284
PRIMER_LEFT_1_PENALTY=0.822824
PRIMER_RIGHT_1_PENALTY=0.005574
PRIMER_LEFT_1_SEQUENCE=ACCACCGGTGTACAAATACAAA
PRIMER_RIGHT_1_SEQUENCE=TGGTGGTGGAGGAGAAGAGTAT
PRIMER_LEFT_1=395,22
PRIMER_RIGHT_1=680,22
PRIMER_LEFT_1_TM=59.177
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=5.3000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=286
PRIMER_PAIR_PENALTY_2=1.0523
PRIMER_LEFT_2_PENALTY=1.046699
PRIMER_RIGHT_2_PENALTY=0.005574
PRIMER_LEFT_2_SEQUENCE=CCATCACCACCACCTTATCACT
PRIMER_RIGHT_2_SEQUENCE=TGGTGGTGGAGGAGAAGAGTAT
PRIMER_LEFT_2=483,22
PRIMER_RIGHT_2=680,22
PRIMER_LEFT_2_TM=61.047
PRIMER_RIGHT_2_TM=59.994
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=5.3000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=1.1091
PRIMER_LEFT_3_PENALTY=1.103525
PRIMER_RIGHT_3_PENALTY=0.005574
PRIMER_LEFT_3_SEQUENCE=CCATCACCACCACCTTATCAC
PRIMER_RIGHT_3_SEQUENCE=TGGTGGTGGAGGAGAAGAGTAT
PRIMER_LEFT_3=483,21
PRIMER_RIGHT_3=680,22
PRIMER_LEFT_3_TM=60.104
PRIMER_RIGHT_3_TM=59.994
PRIMER_LEFT_3_GC_PERCENT=52.381
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=5.3000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=198
PRIMER_PAIR_PENALTY_4=1.1547
PRIMER_LEFT_4_PENALTY=1.149159
PRIMER_RIGHT_4_PENALTY=0.005574
PRIMER_LEFT_4_SEQUENCE=CCCCCACCTGTATACAAATATCA
PRIMER_RIGHT_4_SEQUENCE=TGGTGGTGGAGGAGAAGAGTAT
PRIMER_LEFT_4=423,23
PRIMER_RIGHT_4=680,22
PRIMER_LEFT_4_TM=59.851
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=43.478
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=5.3000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_06060 Ps_contig_mira-and-tgicl-ass_franssen_41429
SEQUENCE=AAGGCGAAGACGATCCACGTAAGAATGCACAAGTATTCATCAACCTCTTCATCCAAAAGGTGGTTTAATCCAAATATTGTATCTTGACTTATGAGTAGAAATTCTAGATTTAAATTGTGATTGTAAGTTGTAACCAAAGGAAGTGGCACAAACGGTTAAGGATTGAAACTTTTACTTTCATTTTCTTATGAATGAAGGTTCAAACCCATCTAAAGCCACCAGAATACATGACTAGATTATGTTGATGATTTCAGATCAATTTTATCAGTGAAATGAAATCATCAACATATCTATCTATCTATGTATCTGACTTTTTCAAATTTCGAAAATATATAAGTAGGTCCGATAAATTTCAAGTTTATTTGTTTAGTCACTTGCCAACACTTGTAAATAGTAATGCCAGCATGCTAGCTTTGGCTTATCAGTAGATGCAACAATCACCACATCCTTCACTTCAAGATGCAGCAACTGTTCTTCCCCATATTGCAGACAGCTCGATGCAAATTTTAACGTATGGGTTCTCAGGGCTGCAARTAGAGACAAACGTAAGACACCGCTATCTGTAAANTGGAGGCGACCCATATGTGATATATACTAGACCCGGATATGAAGTTGATCTTGATTCTCTTGTACGAGATGCCATCAGGCTAAACTCATCAGTACAAGTATATTTAGCAGAATTTCAATTCATGAGTTGTTAACTAAAATTTTGTTAG
TARGET=288,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06061 Ps_contig_mira-and-tgicl-ass_franssen_41432
SEQUENCE=ACTTTTTATTGTAATCCTCTGATTCATCTCATCATATATATATCACAAAAGTTTACACTACACTACAATAATCATGCAACCACTTGTATCATAACAAAATAACATTGAAATCGGTTCGGTGCTACCATTTGTATACAGTAATAGGAAATAAGAAATTTATGAATTGGTTACAGAAAGCAACATTGTAAGCAAATGTCATTTTGATTGGTAGGCCAGTTGGTATATAACACAGGATATGGTTTAATTTCGCTTGAATACAATGATAACTGCAGTGCAGTTATCTTTGCATCGACGCTCTCGAACAGCTTCCCTTATAAGACGGCGGCTCACAACTGTTACAGATAGTCCCTCATTCAACAACTTCTGAACAAAATCAACAGCATCACTGGGACCAAATACCCCCCACAATCCATCGCAACCAAGAATGATGAAATGATCTCTATCAGTAAGGTCAAATGAATGGATATCTGGGGTCGCAACAAGACCCACCTTTTTGAACTGCCGATCTCCAAAAGCCCTAGATACTTCAAGACGCCCTTGTAATCGTCCATTTGAGCTCACAGCACCACCAGCCTAGAATATATCAGGCTAGAAAACTAATTGAGGTGACAACTCCAACAGCACATATACACATATATAGATAGTAATAATTTCACTCTTAAGTCACCAATTTTAAAATGAAACGCATCACACAAAAAATGAATACTTCAATTACTTCCTGGTCCCGCTGGGACTTGAATTGGTCAAGCTGCTGGTAACACAAAGTGATGGATATGGCAAAAAGCCATAATATGTGTGCATAGCTTAATCTCAAACTGTTTCATTTAGCTCAATAATGAGAATTGAAAACATGTTTACAGAGAGTGCAGTTAAGTTCTCTATGTGTAATAGTAGAAAATGGAAACGTTAATGGATAGCAGGAAATCAGGGTCTTATTTGTTAACTGACTGCAATAAAAGAAAACGTACAAAGTAAAAGGTATTGGACATTAGGTAAAAAGAAATTGGTGATAATAATAACG
TARGET=34,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06062 Ps_contig_mira-and-tgicl-ass_franssen_41433
SEQUENCE=TCTGCCTTCTATCTGTTTACTCTTCTCACTCTGAGGAGCTAATTCTATTCCATCTTCCAGTTCCTTCTCAATGAGATCATCATCATCCATGATATCTGTTTTTTNGTTATATGACCAGCTGCTTTCCTTCGTTTTTCTAATGCAGCCTTTACCTTGTCTCTATCGATCTTTTTTATGGCTTCTTGAGGAGGCCTGTTGATTATACTATCCCTGTGATCTGAGTTTCTGCTGTTATGTTTATCTCTCAATTCAGCATGCTCTTTAGTTTCAGTTCTATGAAAATTGTTTTCTAGCTCTTTCTCACCGTTATCA
TARGET=76,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3602
PRIMER_LEFT_PENALTY=0.309418
PRIMER_RIGHT_PENALTY=0.050763
PRIMER_LEFT_SEQUENCE=TTCCATCTTCCAGTTCCTTCTC
PRIMER_RIGHT_SEQUENCE=CAGCAGAAACTCAGATCACAGG
PRIMER_LEFT=47,22
PRIMER_RIGHT=230,22
PRIMER_LEFT_TM=59.691
PRIMER_RIGHT_TM=60.051
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=184
PRIMER_PAIR_PENALTY_1=0.3908
PRIMER_LEFT_1_PENALTY=0.309418
PRIMER_RIGHT_1_PENALTY=0.081412
PRIMER_LEFT_1_SEQUENCE=TTCCATCTTCCAGTTCCTTCTC
PRIMER_RIGHT_1_SEQUENCE=AGGCTGCATTAGAAAAACGAAG
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=147,22
PRIMER_LEFT_1_TM=59.691
PRIMER_RIGHT_1_TM=59.919
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=101
PRIMER_PAIR_PENALTY_2=0.5778
PRIMER_LEFT_2_PENALTY=0.309418
PRIMER_RIGHT_2_PENALTY=0.268401
PRIMER_LEFT_2_SEQUENCE=TTCCATCTTCCAGTTCCTTCTC
PRIMER_RIGHT_2_SEQUENCE=CCTCCTCAAGAAGCCATAAAAA
PRIMER_LEFT_2=47,22
PRIMER_RIGHT_2=190,22
PRIMER_LEFT_2_TM=59.691
PRIMER_RIGHT_2_TM=59.732
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=144
PRIMER_PAIR_PENALTY_3=0.6516
PRIMER_LEFT_3_PENALTY=0.600817
PRIMER_RIGHT_3_PENALTY=0.050763
PRIMER_LEFT_3_SEQUENCE=CCATCTTCCAGTTCCTTCTCAA
PRIMER_RIGHT_3_SEQUENCE=CAGCAGAAACTCAGATCACAGG
PRIMER_LEFT_3=49,22
PRIMER_RIGHT_3=230,22
PRIMER_LEFT_3_TM=60.601
PRIMER_RIGHT_3_TM=60.051
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=182
PRIMER_PAIR_PENALTY_4=0.6945
PRIMER_LEFT_4_PENALTY=0.309418
PRIMER_RIGHT_4_PENALTY=0.385098
PRIMER_LEFT_4_SEQUENCE=TTCCATCTTCCAGTTCCTTCTC
PRIMER_RIGHT_4_SEQUENCE=ATAATCAACAGGCCTCCTCAAG
PRIMER_LEFT_4=47,22
PRIMER_RIGHT_4=202,22
PRIMER_LEFT_4_TM=59.691
PRIMER_RIGHT_4_TM=59.615
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=156
=
PRIMER_SEQUENCE_ID=SSR_06063 Ps_contig_mira-and-tgicl-ass_franssen_41438
SEQUENCE=TTCGATAAGAGGAGGGCTCTGCTATTTGGTGGTGTTGTGGATATCGAAGTTGAAGGTGATGCAATGATGAGCTTGTTCTTGAATGAGTTTTATGGCTTTCAGTTAGACACCAACCGCTGGTATCCTCTGGAGTTAAGAAAGGATAAATCAACAAAAGATAAGTTAAGAAAGACTGAACAAAGTTGTACCAACGGCGTCGAGAAGAAGTTAGAATCACTAGAGGATTCAGAGTACGAAGAAAGCAGCATTGAAAATATATCCCAGGATATTGCGTCAAAGGTGACTGTTGCTGACAGTGAAACACTTTCCAAACCTGAAGGGAAGTCCAAGGAATCTGGTGCTAAATTGGACATTCAGAGTTCTTTGCCTGAGGCAGTGAAACCCTGTGGACGGATTAATTCTTGCATGGCTGTTGGAAGAGATACATTATATGTATATGGTGGCATGATGGAGATTAAAGATCAAGAAATCACTCTTGACGACTTGTATTCTCTGAACCTTAGCAAACTTGATGAATGGAAGTGCATTATACCGGCATCAGAATCTGAATGGATGGAAGTTTCGGAGGGTGACGATGATGATGAAGATGATGACGACGATGATGATGAATCTAATAGCGATGGTACAAGTGAGGAGAACGAAGATGATGATGATGATGATGAAGATGAGGTTCAGAATACATCAGTTCAGGTGGGAGATGCTGTTGCTTTAATCAAGGGAGGAGTAAAGAATCTGCGTAGGAAAGATAGAAGGTTACGGATAGAGCAAATCAGAGCTAGCCTTGGCCTGTCAGATTCACAGAGAACACCATTGGCAGGTGAATCGTTGAGAGATTTCTACAAGCGTACAAATTTGTATTGGCAAATGGCAGCCCATGAACACACTCAGCACACTGGAAAAGAACTCCGCAAAGATGGTTTTGATCTTGCAGAATCTCGATACAGGGAGCTAAAACCTATTCTTGATGAGCTGGCTTTATTAGAAGCTGAGCAGAAAGCTGAAGAGGCTGAGGGGCCTGAGATTAATGCAAAGAAAAGAGGCAAGAAGAAAACTAGAAATTGATAATTCTAATGCAACTCTCCCTTGAGGCTAGGGTATCACAGTGAATGAAGTAATTTGCAAATTTTGTTTTAGTATATGATAGTACCTGCATCTG
TARGET=643,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1730
PRIMER_LEFT_PENALTY=0.070748
PRIMER_RIGHT_PENALTY=0.102276
PRIMER_LEFT_SEQUENCE=ATGTATATGGTGGCATGATGGA
PRIMER_RIGHT_SEQUENCE=GATTAAAGCAACAGCATCTCCC
PRIMER_LEFT=430,22
PRIMER_RIGHT=713,22
PRIMER_LEFT_TM=59.929
PRIMER_RIGHT_TM=60.102
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=284
PRIMER_PAIR_PENALTY_1=0.2490
PRIMER_LEFT_1_PENALTY=0.146683
PRIMER_RIGHT_1_PENALTY=0.102276
PRIMER_LEFT_1_SEQUENCE=TGAATGGAAGTGCATTATACCG
PRIMER_RIGHT_1_SEQUENCE=GATTAAAGCAACAGCATCTCCC
PRIMER_LEFT_1=512,22
PRIMER_RIGHT_1=713,22
PRIMER_LEFT_1_TM=59.853
PRIMER_RIGHT_1_TM=60.102
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.9000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=202
PRIMER_PAIR_PENALTY_2=0.2728
PRIMER_LEFT_2_PENALTY=0.225996
PRIMER_RIGHT_2_PENALTY=0.046801
PRIMER_LEFT_2_SEQUENCE=TGGTACAAGTGAGGAGAACGAA
PRIMER_RIGHT_2_SEQUENCE=TCTTTTCCAGTGTGCTGAGTGT
PRIMER_LEFT_2=620,22
PRIMER_RIGHT_2=901,22
PRIMER_LEFT_2_TM=59.774
PRIMER_RIGHT_2_TM=59.953
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=282
PRIMER_PAIR_PENALTY_3=0.2768
PRIMER_LEFT_3_PENALTY=0.174518
PRIMER_RIGHT_3_PENALTY=0.102276
PRIMER_LEFT_3_SEQUENCE=TATGGTGGCATGATGGAGATTA
PRIMER_RIGHT_3_SEQUENCE=GATTAAAGCAACAGCATCTCCC
PRIMER_LEFT_3=435,22
PRIMER_RIGHT_3=713,22
PRIMER_LEFT_3_TM=60.175
PRIMER_RIGHT_3_TM=60.102
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.9000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=279
PRIMER_PAIR_PENALTY_4=0.3163
PRIMER_LEFT_4_PENALTY=0.214036
PRIMER_RIGHT_4_PENALTY=0.102276
PRIMER_LEFT_4_SEQUENCE=GATGACGACGATGATGATGAAT
PRIMER_RIGHT_4_SEQUENCE=GATTAAAGCAACAGCATCTCCC
PRIMER_LEFT_4=588,22
PRIMER_RIGHT_4=713,22
PRIMER_LEFT_4_TM=59.786
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=126
=
PRIMER_SEQUENCE_ID=SSR_06064 Ps_contig_mira-and-tgicl-ass_franssen_41450
SEQUENCE=ACTTCCAAATAGCAAAAGACAGATATTATACATTGTATCCCCTTCTGATGGAAGATTCTTAAAAGTGACGTACAAGAATAATCAATACAAATTGAATATAAGATTACATACATACATAAATCTGCCATACCATTCTCAGTGCAAACACATAATATATGACCAACTACTACAATACAATATTTCCATGCCCTATGTTGAACCAGTGTGAACTAAGAAGAAAATGGTATATCTTTTCTCATAAAAAAATCTTTTAAGTTCAAACCCAAAACACTCACAAGCTCCCTGTTCTTCAAACATCAACATCTGAACGTGTTGGAAACTCGATGCCCCTTAGTGCAGGTATGTTGACTAAATCCTCTTCTGAAAACAATGGAAGCAACCATAGGGCCTTCTTTGTGCCAAAAACCTGCTCAAAATTTGTCTTCCTTCCCAAGTCGTACCTCCACATGATTCCTTTTTTCTTTTCATGAACCTCTACTGAAGTTGTGTTGCTTAACAGAAGAGATAAATGCATAACTACGAAACAGAGAAGACTCAGCGCAAAGGCTAAATTGAGAATAGAAGTCAAGAAAATTGCAGAAAAGGCCCCGGGAGACAATTTACGAGTCTTGGTTAAGTTGGTTCCCCCAAAGAATCTAATAAAATCAGGTATCAGAGCTAAGCAAACCAGTGTTGTCTCGAGGAATGTATACAGCAAAAAGAGAAGAAAATACTTGTAATTCCGTGCCCCGACACAATTAACCACCCAAATACAATGATGATCCATCTTAAGAACACACCTTTGACAAATGGAGCAGTGATGACAACGTGGTGGCCTGGCGCTTTGGCACCGAGTACAGTAACCGGCGGAGGATGTTGCTGTTGTTATCACCGCCGCCTCATCGAACTCGCGGGAAGGTGGTGGACCTGCAACATGTAAATTGCGGTGATCATCTAGCTCCACCTGTAAAAACTGTTGATGCGGTCTCCAATTTTGAGGAACAGAACCTGGATCCTTGAGAACCGCCATAGAATAGGACCAAGTTAACAGCACAAGGAGAGTGTGAAAGATGAGGAGAACGAAGAAGGCTAAGAAGGAGAAAGATGGAAACAAGAGCGGGCCCCACGTGACGAAAACGACGGCGTAGTAGGTGAGAGAAACGATAGCGGCGAACAAGAGGATCATGAGGTAGCCAAGGACTCTGAGAAAGGAGCAAAACTTGAAGAGGTTAATGTTAAGCATTGTTGGGAATGGTGGGAGAATTCGTCTACTGGTTCTTCGTGTTGGGAAGAGGCGTTTCCATGTATCTGAAGAAGCCAGGATGGATTGATAGATTGGTATGCCTCTTTGGTTCTGCCACTTTGGGTTTGGGGTTTCACTTCCACTTT
TARGET=105,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3423
PRIMER_LEFT_PENALTY=0.334070
PRIMER_RIGHT_PENALTY=0.008257
PRIMER_LEFT_SEQUENCE=ATTGTATCCCCTTCTGATGGAA
PRIMER_RIGHT_SEQUENCE=TGTTGATGTTTGAAGAACAGGG
PRIMER_LEFT=31,22
PRIMER_RIGHT=301,22
PRIMER_LEFT_TM=59.666
PRIMER_RIGHT_TM=60.008
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.3792
PRIMER_LEFT_1_PENALTY=0.334070
PRIMER_RIGHT_1_PENALTY=0.045112
PRIMER_LEFT_1_SEQUENCE=ATTGTATCCCCTTCTGATGGAA
PRIMER_RIGHT_1_SEQUENCE=CTTGTGAGTGTTTTGGGTTTGA
PRIMER_LEFT_1=31,22
PRIMER_RIGHT_1=277,22
PRIMER_LEFT_1_TM=59.666
PRIMER_RIGHT_1_TM=60.045
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=7.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.4089
PRIMER_LEFT_2_PENALTY=0.334070
PRIMER_RIGHT_2_PENALTY=0.074807
PRIMER_LEFT_2_SEQUENCE=ATTGTATCCCCTTCTGATGGAA
PRIMER_RIGHT_2_SEQUENCE=AGCTTGTGAGTGTTTTGGGTTT
PRIMER_LEFT_2=31,22
PRIMER_RIGHT_2=279,22
PRIMER_LEFT_2_TM=59.666
PRIMER_RIGHT_2_TM=60.075
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=249
PRIMER_PAIR_PENALTY_3=0.4114
PRIMER_LEFT_3_PENALTY=0.334070
PRIMER_RIGHT_3_PENALTY=0.077340
PRIMER_LEFT_3_SEQUENCE=ATTGTATCCCCTTCTGATGGAA
PRIMER_RIGHT_3_SEQUENCE=GAAGAACAGGGAGCTTGTGAGT
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=290,22
PRIMER_LEFT_3_TM=59.666
PRIMER_RIGHT_3_TM=59.923
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=260
PRIMER_PAIR_PENALTY_4=0.5393
PRIMER_LEFT_4_PENALTY=0.334070
PRIMER_RIGHT_4_PENALTY=0.205226
PRIMER_LEFT_4_SEQUENCE=ATTGTATCCCCTTCTGATGGAA
PRIMER_RIGHT_4_SEQUENCE=GTTTGAAGAACAGGGAGCTTGT
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=59.666
PRIMER_RIGHT_4_TM=59.795
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=264
=
PRIMER_SEQUENCE_ID=SSR_06065 Ps_contig_mira-and-tgicl-ass_franssen_41451
SEQUENCE=TCGTCGGGGATTCATTCATTCATTCATCTCTGGAGTGTCATGTCACCCAACAACTAGTCGCCATTACACCACTCAACGGAGCTCATGGCGGGAACCTCCCATTTACTCATCCTCACTCCGCACTCCGCCACCACTTCCTCCTTCCCTCGTCGAATCCCTCCATTTCTCGCTTCCCGCCGTCATCTTCACTCACCTGTTAAACCCCTCACTCTACGCCCCGCTGTTTGTATCCCATCTCCCTTCAGAGCATGTGCTCAATCCCATCAGRTTCCATTTCTCCTTCATCGTCGTGATTATAAGCTTCAGCGAAAAGGAAACAAAATA
TARGET=10,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06066 Ps_contig_mira-and-tgicl-ass_franssen_41454
SEQUENCE=CGTTAGTGGCTAAAGATGYCTYTAACTATAAGATTGTTTGTCACAATTATGAATGAAAGTGAGTACAACNAAAAATGTATCTATATCCCCACCATTAAAGGAGAAAAAAGATGATTATGAAAAGTAAGGAAAGAAATCAATATGGTTTATACAGAAGAGAAATGCTTAACCATTTACAAGTGGTTCACTGATTTCAAGCAATTCATTTCTGAGACTAGTGAAAACAACAGAATCTGTTTCTAGGGGCTTGAACTTGATTAGAGCAGAAGGTACCAAGTCTTCTTCCTCTATTGTTTTGGCTTTTTGCCCGGCTTTTGGAAAACATGGGATAACTCGCCGTTGAACAACTACAGGATGCATTAACTCGAATTCCAAACCTTGTTCTTTTAATGCAGAGCTGACAAACTCATACAAAGCAGTAGTTGGTTCCCACGGAGCAAATACACCTTGAAGTACAATTCCATCTGGAAACTGAACCCTAATGATAGTTCTTGTGTATCGCCTCTTCGCAGCTTTAGCTTGTTTTTCTTTAAGGGACTTGGGGATTAAAAGCTGAGACTCTTCAAACTTTTTTCTCCTTGATTCAGCATCTCTTCTCACTTCATCAACTGATCGTTTGTAGAAGGAATCTGGTAGCTCAATTTTAGCTGCAACGCTTTCAGAGACAGCAAAGAAGACCTTGACCTGTCTGTCGATCTTTTTCGGTTCAACATCGGCTACTTTCTCAACAGAAGTTGTAGACACTAAACTATCTTTCTTTGGAGGATCCTTTACCAATTGTGGTTCCAACAACACAATTGCTTTCTTGATCAAATCTATCTTCTCCTCATTAGGAACCTCCATCAANCCCCATGTCTCTCCATTCTCCTCCTTAAGTCCAAAACCCAAAAAGCTCAACAACTCAACCCCACCACTAACATCAACAACAGCTTCCTTAATCTTGGGATTACTCAGCCTAATCTTCCTAAACTTAACATTCTCAGGCTCCTTAACAATATTCTTCAACAACTTCAAAACAATATCAACCGATCCTTCCGACGGATTCCCCGAAACATAACTCCCAACACAAATCTCCAATTCAGTATTACTCTCAACAACAACTTCACTCTCCACCAATCTTCCATCATCATCATTTCTAACTGAATTACTCAAACAACTTTCCACATGTTCAGAAACCTCATCTTCAGACCTAAACGGAGTTTTACAAATAGGACATTCATATACATTAACTGAATATCCATTTCGAGATCTCTGAGATGAAGTGACCAAAGAATCAAACGGATCAAATCCATCTCCATGCTTACGAGCTTTTTCAATCTTGCTATCATCAGAAGCTGAGGTAATCGGTAGAGTGGGTTCGGGTTTGGGTTTGGGTTCGGGTTTAGGTTTTGAAATAGGATTGGAATTCTGAGAAGGAGAGGGGCGAGGGGTAGAAATGGAATTAACCGGTGTAGATGATGATGAAGAAGACGAAGAAGAGCCAAGAACTCTACCTTGGCCTTTGAATTTTCCAGAGGATGAAGATGAGAAAGGGTTATTGACTTTCTTGATGAAACCTTTCATCTTGTCTTTTACATCGTCCATTTTTGTTCTATGATGTAAAAACCCTAATTCAATGGAGAAACAGAATTGGATCTGAAATGGTTAATAGTTGAACCGAATTTGTGGTAGTAGTGATTGGTGTAAGGAACTTAGGGGGTAAGGAACATGAAAGAGAGGAAAATGGAGAATCTTTAGAAGAGATTGACTTCATGAAATTTATCTTTTTGTGAGGAAAAT
TARGET=1122,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06067 Ps_contig_mira-and-tgicl-ass_franssen_41461
SEQUENCE=TTTTCTTAAATTAAAATTATAAACTTTCTCTGYTANCTAAAACAAATGCTTTTTATTAGCTTTCTTGAATTTCTCTTAGCCTCAAGAAAATAATCAAGACTAATCTACATTCTCATTGCTCATGAATAAGGAGCACAAAAACAAAATAGTAGAAAGACAAAGACACATACATCTTAATATACTATATATAATATACTACTTGATGATTTTTCTTATCTTATTCTAAAAAATGGAAGTAAAAGAAAAAGAAAAGAAAAGAAGAAGAAGAAGAATTCCTTTGTTGCTTCAATGCATTGAACAAGTTCATCACCTTTTTCTATTTTATTATTCAAAACGCTTTTCTTCAGATTCACTTTCACTAGCTAATGAAAGGAAAAACCCAGTTTAGCATATTACTTTTTTTTGGTGACTGTTGTTACCTCTTCAAATATGAAAATGGTATAGGTAAATGCTTCAAGCTTGAAATATCAAACAACTCTACAAACCTCTGATCCGAAACTCGAGCTTTCGCAGCTGCAAACCGTTGGTACTCGTCGAAAACAGACGACAAGCACCATTTTTGGAGCTTTCTATAGCATCCGACTAAACAACCTGTTCGGTGCTTTCCTCGCTTGCAGTGAATTATAACCGGGTGGTTCTTTACGTCAAGAAGAACTTTTAGGGCTTCCCGAATAGTGTCGTCCGGGATGTTTACAAAAGGCTCCTTATGACCCTCAATTCCAAAGTGAAAAAGCTTGATTCCATTTGACTTGAGAAACTCCATGTTAGCTTCTGGATATGGTTCAGGGCACAAATATATGATGGAGCGAAGGCCAAGGGTTTGAAGGAAAGAGAAGTTGGAAGGTTCAGGGAAACCAGAACGGAAAATGCCGTTATCAACCATAGAAAAATTGAGAGGTGGAATGAAGAGATCCTCGCCGTCAATTTTGTCACAAACATCATCGTCATCRACGGTGGTTGTGAGTTTGAGGTTGTTGTGATCAGTGATACTGAGTTTGATTTTTCGACACATCTGTGTTTCTTGGTTCTTTTTCTTGAGTTGGTGGTGTTGAACTTCTGATGCCACTTGTTGTTGCATATTAAGAGAGAGAGAGAGAGAGAGAGGT
TARGET=244,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06068 Ps_contig_mira-and-tgicl-ass_franssen_41461
SEQUENCE=TTTTCTTAAATTAAAATTATAAACTTTCTCTGYTANCTAAAACAAATGCTTTTTATTAGCTTTCTTGAATTTCTCTTAGCCTCAAGAAAATAATCAAGACTAATCTACATTCTCATTGCTCATGAATAAGGAGCACAAAAACAAAATAGTAGAAAGACAAAGACACATACATCTTAATATACTATATATAATATACTACTTGATGATTTTTCTTATCTTATTCTAAAAAATGGAAGTAAAAGAAAAAGAAAAGAAAAGAAGAAGAAGAAGAATTCCTTTGTTGCTTCAATGCATTGAACAAGTTCATCACCTTTTTCTATTTTATTATTCAAAACGCTTTTCTTCAGATTCACTTTCACTAGCTAATGAAAGGAAAAACCCAGTTTAGCATATTACTTTTTTTTGGTGACTGTTGTTACCTCTTCAAATATGAAAATGGTATAGGTAAATGCTTCAAGCTTGAAATATCAAACAACTCTACAAACCTCTGATCCGAAACTCGAGCTTTCGCAGCTGCAAACCGTTGGTACTCGTCGAAAACAGACGACAAGCACCATTTTTGGAGCTTTCTATAGCATCCGACTAAACAACCTGTTCGGTGCTTTCCTCGCTTGCAGTGAATTATAACCGGGTGGTTCTTTACGTCAAGAAGAACTTTTAGGGCTTCCCGAATAGTGTCGTCCGGGATGTTTACAAAAGGCTCCTTATGACCCTCAATTCCAAAGTGAAAAAGCTTGATTCCATTTGACTTGAGAAACTCCATGTTAGCTTCTGGATATGGTTCAGGGCACAAATATATGATGGAGCGAAGGCCAAGGGTTTGAAGGAAAGAGAAGTTGGAAGGTTCAGGGAAACCAGAACGGAAAATGCCGTTATCAACCATAGAAAAATTGAGAGGTGGAATGAAGAGATCCTCGCCGTCAATTTTGTCACAAACATCATCGTCATCRACGGTGGTTGTGAGTTTGAGGTTGTTGTGATCAGTGATACTGAGTTTGATTTTTCGACACATCTGTGTTTCTTGGTTCTTTTTCTTGAGTTGGTGGTGTTGAACTTCTGATGCCACTTGTTGTTGCATATTAAGAGAGAGAGAGAGAGAGAGAGGT
TARGET=256,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06069 Ps_contig_mira-and-tgicl-ass_franssen_41461
SEQUENCE=TTTTCTTAAATTAAAATTATAAACTTTCTCTGYTANCTAAAACAAATGCTTTTTATTAGCTTTCTTGAATTTCTCTTAGCCTCAAGAAAATAATCAAGACTAATCTACATTCTCATTGCTCATGAATAAGGAGCACAAAAACAAAATAGTAGAAAGACAAAGACACATACATCTTAATATACTATATATAATATACTACTTGATGATTTTTCTTATCTTATTCTAAAAAATGGAAGTAAAAGAAAAAGAAAAGAAAAGAAGAAGAAGAAGAATTCCTTTGTTGCTTCAATGCATTGAACAAGTTCATCACCTTTTTCTATTTTATTATTCAAAACGCTTTTCTTCAGATTCACTTTCACTAGCTAATGAAAGGAAAAACCCAGTTTAGCATATTACTTTTTTTTGGTGACTGTTGTTACCTCTTCAAATATGAAAATGGTATAGGTAAATGCTTCAAGCTTGAAATATCAAACAACTCTACAAACCTCTGATCCGAAACTCGAGCTTTCGCAGCTGCAAACCGTTGGTACTCGTCGAAAACAGACGACAAGCACCATTTTTGGAGCTTTCTATAGCATCCGACTAAACAACCTGTTCGGTGCTTTCCTCGCTTGCAGTGAATTATAACCGGGTGGTTCTTTACGTCAAGAAGAACTTTTAGGGCTTCCCGAATAGTGTCGTCCGGGATGTTTACAAAAGGCTCCTTATGACCCTCAATTCCAAAGTGAAAAAGCTTGATTCCATTTGACTTGAGAAACTCCATGTTAGCTTCTGGATATGGTTCAGGGCACAAATATATGATGGAGCGAAGGCCAAGGGTTTGAAGGAAAGAGAAGTTGGAAGGTTCAGGGAAACCAGAACGGAAAATGCCGTTATCAACCATAGAAAAATTGAGAGGTGGAATGAAGAGATCCTCGCCGTCAATTTTGTCACAAACATCATCGTCATCRACGGTGGTTGTGAGTTTGAGGTTGTTGTGATCAGTGATACTGAGTTTGATTTTTCGACACATCTGTGTTTCTTGGTTCTTTTTCTTGAGTTGGTGGTGTTGAACTTCTGATGCCACTTGTTGTTGCATATTAAGAGAGAGAGAGAGAGAGAGAGGT
TARGET=1083,22
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06070 Ps_contig_mira-and-tgicl-ass_franssen_41466
SEQUENCE=AAGGTTAGNTTAATCAAAATTATATGATGACGATGATGTTCAAGAATCTTACTTACAGAGTAATTTACTTACAACATAATAATATCCCTTTTCTCTCTACTTCAAGTGTCAAATTTGCACTTATATTACACAAATACCTATATGATTGATTGATTGATTTGGGATATGAAGATAAAATAAAATAAAAATAAGAACATGTATAAAATGTTACTAACGCTAAAGGGAGCTGCATGAACAACAAACTCTAGACCGAACCGGTCAACTAGGATGGGCGGTTCGGTTGTCGTACTGTAATTCTAGCAGGTAAAGGTCGACCGGTTCCGGACCCACCACAACTGCTGCAACCCATGCGACCCGAACCGGAACACGTACTACAGCCGACATCACCGTCAGACCAGCGTGAGCAGAGACAAGGGACTCTACCGGTTCCGTTGCAGCTGGGACATCTGGGAAATTGACCCGCCATGGGTTTTTGGTCCATCACCGATTTAACGAAAACCGCTCCGGCCAGGACGCTTAGACCGGTGGCTAATTGGCTCAGCACGAGTGGACCCATTTGGTTGAATAGAAGATACGAGGAGATATGAAGATGGATAAACAGAGAGAGTTAGAAGAGGAAAGAGAAGAGATAAAGATTTTGTGTGAAAGTTTTATTTGGAGTGGTGAATCAG
TARGET=143,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.0045
PRIMER_LEFT_PENALTY=3.941772
PRIMER_RIGHT_PENALTY=0.062749
PRIMER_LEFT_SEQUENCE=CCCTTTTCTCTCTACTTCAAGTGTC
PRIMER_RIGHT_SEQUENCE=GCAGCTCCCTTTAGCGTTAGTA
PRIMER_LEFT=85,25
PRIMER_RIGHT=229,22
PRIMER_LEFT_TM=59.058
PRIMER_RIGHT_TM=60.063
PRIMER_LEFT_GC_PERCENT=44.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=4.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=4.0045
PRIMER_LEFT_1_PENALTY=3.941772
PRIMER_RIGHT_1_PENALTY=0.062749
PRIMER_LEFT_1_SEQUENCE=TCCCTTTTCTCTCTACTTCAAGTGT
PRIMER_RIGHT_1_SEQUENCE=GCAGCTCCCTTTAGCGTTAGTA
PRIMER_LEFT_1=84,25
PRIMER_RIGHT_1=229,22
PRIMER_LEFT_1_TM=59.058
PRIMER_RIGHT_1_TM=60.063
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=4.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=4.0148
PRIMER_LEFT_2_PENALTY=3.941772
PRIMER_RIGHT_2_PENALTY=0.073038
PRIMER_LEFT_2_SEQUENCE=CCCTTTTCTCTCTACTTCAAGTGTC
PRIMER_RIGHT_2_SEQUENCE=GAGTTTGTTGTTCATGCAGCTC
PRIMER_LEFT_2=85,25
PRIMER_RIGHT_2=244,22
PRIMER_LEFT_2_TM=59.058
PRIMER_RIGHT_2_TM=59.927
PRIMER_LEFT_2_GC_PERCENT=44.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=4.0148
PRIMER_LEFT_3_PENALTY=3.941772
PRIMER_RIGHT_3_PENALTY=0.073038
PRIMER_LEFT_3_SEQUENCE=TCCCTTTTCTCTCTACTTCAAGTGT
PRIMER_RIGHT_3_SEQUENCE=GAGTTTGTTGTTCATGCAGCTC
PRIMER_LEFT_3=84,25
PRIMER_RIGHT_3=244,22
PRIMER_LEFT_3_TM=59.058
PRIMER_RIGHT_3_TM=59.927
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=4.6733
PRIMER_LEFT_4_PENALTY=4.610527
PRIMER_RIGHT_4_PENALTY=0.062749
PRIMER_LEFT_4_SEQUENCE=CCCTTTTCTCTCTACTTCAAGTGT
PRIMER_RIGHT_4_SEQUENCE=GCAGCTCCCTTTAGCGTTAGTA
PRIMER_LEFT_4=85,24
PRIMER_RIGHT_4=229,22
PRIMER_LEFT_4_TM=57.389
PRIMER_RIGHT_4_TM=60.063
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=4.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_06071 Ps_contig_mira-and-tgicl-ass_franssen_41466
SEQUENCE=AAGGTTAGNTTAATCAAAATTATATGATGACGATGATGTTCAAGAATCTTACTTACAGAGTAATTTACTTACAACATAATAATATCCCTTTTCTCTCTACTTCAAGTGTCAAATTTGCACTTATATTACACAAATACCTATATGATTGATTGATTGATTTGGGATATGAAGATAAAATAAAATAAAAATAAGAACATGTATAAAATGTTACTAACGCTAAAGGGAGCTGCATGAACAACAAACTCTAGACCGAACCGGTCAACTAGGATGGGCGGTTCGGTTGTCGTACTGTAATTCTAGCAGGTAAAGGTCGACCGGTTCCGGACCCACCACAACTGCTGCAACCCATGCGACCCGAACCGGAACACGTACTACAGCCGACATCACCGTCAGACCAGCGTGAGCAGAGACAAGGGACTCTACCGGTTCCGTTGCAGCTGGGACATCTGGGAAATTGACCCGCCATGGGTTTTTGGTCCATCACCGATTTAACGAAAACCGCTCCGGCCAGGACGCTTAGACCGGTGGCTAATTGGCTCAGCACGAGTGGACCCATTTGGTTGAATAGAAGATACGAGGAGATATGAAGATGGATAAACAGAGAGAGTTAGAAGAGGAAAGAGAAGAGATAAAGATTTTGTGTGAAAGTTTTATTTGGAGTGGTGAATCAG
TARGET=172,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.0045
PRIMER_LEFT_PENALTY=3.941772
PRIMER_RIGHT_PENALTY=0.062749
PRIMER_LEFT_SEQUENCE=CCCTTTTCTCTCTACTTCAAGTGTC
PRIMER_RIGHT_SEQUENCE=GCAGCTCCCTTTAGCGTTAGTA
PRIMER_LEFT=85,25
PRIMER_RIGHT=229,22
PRIMER_LEFT_TM=59.058
PRIMER_RIGHT_TM=60.063
PRIMER_LEFT_GC_PERCENT=44.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.1000
PRIMER_RIGHT_END_STABILITY=4.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=145
PRIMER_PAIR_PENALTY_1=4.0045
PRIMER_LEFT_1_PENALTY=3.941772
PRIMER_RIGHT_1_PENALTY=0.062749
PRIMER_LEFT_1_SEQUENCE=TCCCTTTTCTCTCTACTTCAAGTGT
PRIMER_RIGHT_1_SEQUENCE=GCAGCTCCCTTTAGCGTTAGTA
PRIMER_LEFT_1=84,25
PRIMER_RIGHT_1=229,22
PRIMER_LEFT_1_TM=59.058
PRIMER_RIGHT_1_TM=60.063
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=4.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=4.0148
PRIMER_LEFT_2_PENALTY=3.941772
PRIMER_RIGHT_2_PENALTY=0.073038
PRIMER_LEFT_2_SEQUENCE=CCCTTTTCTCTCTACTTCAAGTGTC
PRIMER_RIGHT_2_SEQUENCE=GAGTTTGTTGTTCATGCAGCTC
PRIMER_LEFT_2=85,25
PRIMER_RIGHT_2=244,22
PRIMER_LEFT_2_TM=59.058
PRIMER_RIGHT_2_TM=59.927
PRIMER_LEFT_2_GC_PERCENT=44.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=4.0148
PRIMER_LEFT_3_PENALTY=3.941772
PRIMER_RIGHT_3_PENALTY=0.073038
PRIMER_LEFT_3_SEQUENCE=TCCCTTTTCTCTCTACTTCAAGTGT
PRIMER_RIGHT_3_SEQUENCE=GAGTTTGTTGTTCATGCAGCTC
PRIMER_LEFT_3=84,25
PRIMER_RIGHT_3=244,22
PRIMER_LEFT_3_TM=59.058
PRIMER_RIGHT_3_TM=59.927
PRIMER_LEFT_3_GC_PERCENT=40.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=4.6733
PRIMER_LEFT_4_PENALTY=4.610527
PRIMER_RIGHT_4_PENALTY=0.062749
PRIMER_LEFT_4_SEQUENCE=CCCTTTTCTCTCTACTTCAAGTGT
PRIMER_RIGHT_4_SEQUENCE=GCAGCTCCCTTTAGCGTTAGTA
PRIMER_LEFT_4=85,24
PRIMER_RIGHT_4=229,22
PRIMER_LEFT_4_TM=57.389
PRIMER_RIGHT_4_TM=60.063
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=4.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_06072 Ps_contig_mira-and-tgicl-ass_franssen_41470
SEQUENCE=CCATTTTTAACTAGGATCAGATCTACACCTCATCAACAAAAGAAGCATTCTTTTGGTTGGAACACAAATACTAACTCTACCTTTCACTTCTTCTTCTTCCTCTTCACCACCAAACTCCTCTTGTATCTTCTCATCAACCACATCTATATCAAGATCTTCAAAAATATGTCTCCTTTGACTATAACTCCTCTCACTTTTCTCATCATCCATCATCTCCATCTCCTCATGATGTTCCTCCATCATC
TARGET=87,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3178
PRIMER_LEFT_PENALTY=0.283676
PRIMER_RIGHT_PENALTY=0.034113
PRIMER_LEFT_SEQUENCE=CACCTCATCAACAAAAGAAGCA
PRIMER_RIGHT_SEQUENCE=CATCATGAGGAGATGGAGATGA
PRIMER_LEFT=25,22
PRIMER_RIGHT=230,22
PRIMER_LEFT_TM=60.284
PRIMER_RIGHT_TM=60.034
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=206
PRIMER_PAIR_PENALTY_1=0.3178
PRIMER_LEFT_1_PENALTY=0.283676
PRIMER_RIGHT_1_PENALTY=0.034113
PRIMER_LEFT_1_SEQUENCE=CACCTCATCAACAAAAGAAGCA
PRIMER_RIGHT_1_SEQUENCE=TCATGAGGAGATGGAGATGATG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=228,22
PRIMER_LEFT_1_TM=60.284
PRIMER_RIGHT_1_TM=60.034
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.4267
PRIMER_LEFT_2_PENALTY=0.392624
PRIMER_RIGHT_2_PENALTY=0.034113
PRIMER_LEFT_2_SEQUENCE=AGCATTCTTTTGGTTGGAACAC
PRIMER_RIGHT_2_SEQUENCE=CATCATGAGGAGATGGAGATGA
PRIMER_LEFT_2=43,22
PRIMER_RIGHT_2=230,22
PRIMER_LEFT_2_TM=60.393
PRIMER_RIGHT_2_TM=60.034
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=0.4267
PRIMER_LEFT_3_PENALTY=0.392624
PRIMER_RIGHT_3_PENALTY=0.034113
PRIMER_LEFT_3_SEQUENCE=AGCATTCTTTTGGTTGGAACAC
PRIMER_RIGHT_3_SEQUENCE=TCATGAGGAGATGGAGATGATG
PRIMER_LEFT_3=43,22
PRIMER_RIGHT_3=228,22
PRIMER_LEFT_3_TM=60.393
PRIMER_RIGHT_3_TM=60.034
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=0.6505
PRIMER_LEFT_4_PENALTY=0.283676
PRIMER_RIGHT_4_PENALTY=0.366796
PRIMER_LEFT_4_SEQUENCE=CACCTCATCAACAAAAGAAGCA
PRIMER_RIGHT_4_SEQUENCE=TACAAGAGGAGTTTGGTGGTGA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=124,22
PRIMER_LEFT_4_TM=60.284
PRIMER_RIGHT_4_TM=59.633
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=100
=
PRIMER_SEQUENCE_ID=SSR_06073 Ps_contig_mira-and-tgicl-ass_franssen_41476
SEQUENCE=CTTTAAANYAAAGTCGATAAACATCACTTTTCTAAATATTGAATTCTTACAAGAACCATCAATCACACATTACATAATATAACACCTTATTACACTTTGATAATTCAAGCACCTAACATCATATTTCAACACAAACTCAAACCAAACTCCTAAACAACACATCAATCTATAAAAGTAAAAATAAAAAACTCACAATTTTTCAAACCCTAACATTTGGGCTCAAAAGCAAGAGGCCCTACAGTATAGAGAACAAAAGGAAGACCCTTAGACACAAAAGCTTTCTCAGCCCTAAGCACAGCACCACTAACACCACCATGAAGATTCGAAGGCTTAAGCCCATTAGGTGCACTAACCAAAACAACATTACACTTGTGAGCTGCATAGCTTGTAATGCTCTTTGGGCCTTCAATGTAGAAGTAACCATTCTTATCAGTTTCATCCTTTAACACCAACTTATACTTTGTGTTGTTACATTGAAGCTTCACAACGGCACCAAGAAGTGATGAAGCTCCCAAGAGGGTGTCAGCACCAGGATACTTGCATGATTTGACATAAACAACACCTTGAACAGCAATGAAGCTTCTAGGGATGGGAGTGGGGTGCGGTGGTGAGTGAGCAGGAGGAGGAGGGTGTGATGGTGAGTGAGCAGGAGTCGGAGGGTGCAATGGTGGGTGAGTTGGAACAACAGGTGTGTGAGCAGGGGCAGGAGCAGGAGCGTGGTGATGGCCGTGGGTTGGGGGCTTAGCAGGTGGGTGAGCTGGAGATATGGTTGGGGGATGAAGTGGAGCTGGTGAGTGAGGAGGGTAAGGAGTGTGAACAGGGGAGGGAGCTGGGGAATGTGAGTGGTTGGGGTGGTGATGGTGGTGGTGAGGGGGGTTAGCTGGTGGGTGAACGGGTGACTTAGGTTGGGGGGTGAAGAGGGNTGTGTGTG
TARGET=618,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06074 Ps_contig_mira-and-tgicl-ass_franssen_41477
SEQUENCE=TTAGTTTATTCTAAAGAACAACTAACGATGAAACTAAGAATAAAATTAAATTAGTCATCAAATACAAAGTTTTAGTCTCCTCCCTTCTTCTATCATTATCTTGAACTCATTGAAATTAACCATACCATCTCCATCCATATCAACTTTCCTAATCATCTCTTTACACTCTTCAATCTTCTTCCCTTCCCTTAATCCCAACATATTAAGAACCAAACCTAACTCCTCCTCTGAAATTAAACCATCATTATCTTTATCAAATACATCAAAAGCCTCCTTCAAGTTACCCTCTAATTCTTCCAATTCTCCTTCATTACTATTTACACCCTCTTTCTCTTTTTCCTTTTCCTCACCCCCTCTCATACACTCTCTAGTTAACAAACAAAACTCATCAAAATCAATGAACCCATCTTTGTTTGAGTCAAATTTCACAAGAATATCATCAATCTCTTTCTCGTTCATGAAGATCCTTAGTGATTCCCTCAACTCTTGCTTTGTTATGAATCCATCACCATTCTTGTCAAAAGTTGAAAACACCTTTTTGAGTTCTTCTTCTTCCTTCCCATAATTAGCATCCTTCTCAATCTTGGTCGTTTTGCAACCTTTGTTAATGTTGTTGTTGTTGTTGGGAAACAATATTTGAAGGAAAGTACGGATTTTGTTGGTGGGTATGTAAAAGAATATATTTATGACACCTGCAATGAACAGAACCGCTAGCAAAAGTGTAGCAAGAACCATGTTTTAGTCAAACTTAATGCTACAAAGAGAGATTTGAGATAAGGAAGAAGAGGACGAGGAAGTGAGCAAGCAACTAGATTTTAGAACCACATGAGTATATACAAAACTTTGTCTGCTTCCAATGTTTTTATGTTCATAGTGAAAAAGAAGTTACGCGTGTTTGGTTGTGGTGTCTTTTTTTATCTATCYCCCCNCAAACCGCGTGGA
TARGET=544,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06075 Ps_contig_mira-and-tgicl-ass_franssen_41477
SEQUENCE=TTAGTTTATTCTAAAGAACAACTAACGATGAAACTAAGAATAAAATTAAATTAGTCATCAAATACAAAGTTTTAGTCTCCTCCCTTCTTCTATCATTATCTTGAACTCATTGAAATTAACCATACCATCTCCATCCATATCAACTTTCCTAATCATCTCTTTACACTCTTCAATCTTCTTCCCTTCCCTTAATCCCAACATATTAAGAACCAAACCTAACTCCTCCTCTGAAATTAAACCATCATTATCTTTATCAAATACATCAAAAGCCTCCTTCAAGTTACCCTCTAATTCTTCCAATTCTCCTTCATTACTATTTACACCCTCTTTCTCTTTTTCCTTTTCCTCACCCCCTCTCATACACTCTCTAGTTAACAAACAAAACTCATCAAAATCAATGAACCCATCTTTGTTTGAGTCAAATTTCACAAGAATATCATCAATCTCTTTCTCGTTCATGAAGATCCTTAGTGATTCCCTCAACTCTTGCTTTGTTATGAATCCATCACCATTCTTGTCAAAAGTTGAAAACACCTTTTTGAGTTCTTCTTCTTCCTTCCCATAATTAGCATCCTTCTCAATCTTGGTCGTTTTGCAACCTTTGTTAATGTTGTTGTTGTTGTTGGGAAACAATATTTGAAGGAAAGTACGGATTTTGTTGGTGGGTATGTAAAAGAATATATTTATGACACCTGCAATGAACAGAACCGCTAGCAAAAGTGTAGCAAGAACCATGTTTTAGTCAAACTTAATGCTACAAAGAGAGATTTGAGATAAGGAAGAAGAGGACGAGGAAGTGAGCAAGCAACTAGATTTTAGAACCACATGAGTATATACAAAACTTTGTCTGCTTCCAATGTTTTTATGTTCATAGTGAAAAAGAAGTTACGCGTGTTTGGTTGTGGTGTCTTTTTTTATCTATCYCCCCNCAAACCGCGTGGA
TARGET=609,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06076 Ps_contig_mira-and-tgicl-ass_franssen_41479
SEQUENCE=TTGTAAAACAAAGACTTCGAAGACTGAAGACCCGGAGCAACTGTAAAAAGACTTCTCATCAATAAGAACACTGTTGAACTAACTCCAATCAACAAGTAAACTAGAATCAGTCTCAAAGTGCTGACTTTAGGATTGTCAACATTAGCAGCCATCCACGAGTTTTGCAATATCTGACCAATCACAAATATAAGGTGAAATAGAGAAGCGATCAAGAAGTATGCGTATCCTTTGTTCTGATTCAAATACTGTAAGTATGGACTTAAGGCCATGGTCTCCTATCTCTCTCTCTTCT
TARGET=279,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06077 Ps_contig_mira-and-tgicl-ass_franssen_41485
SEQUENCE=AGTAAAATGGGTTACTTTTACATTTTGTTTGGAGGTTTGAAAATATAAATCAAATTCAAACATATTTTACACGTATTTGTAATCTCGTTTTGTAATCCTGCCACAATATAAATAGGATTTTTCTTATTTTCTAATATAAAATTAAATTGTTTAAATGTTCATTTTGATACAATCCTCAAGATCAAGAAAATGTTGTCTTTCAAACTTTCATGCTATCTTCTTTCCATTTACAAAAGACTGCAGAGAGACAGATTCTAGAAGTCTGAGTAATAAATACAAACTAGACTTGAGTGTGTTATAGGGGTGCGGCAAAGAGGGCACTCTGGCTTCTCGTTGCACCATTCTGTGATACAGTTCCAGCAAAATACATGGCCACATGAAGTGGCTGTGGGGTGTTGACGATTACTCAGGCAAAGTGTGCATTTGCTAACACCACTGGTTGCATGAGGCTCAGAAGATGAAGATCCAGAGACCCAACTTCCTTCATCAGCTTCTGGAGATGCCAAATTACCTTCTTCGTTTAGAACAGGCAAACCGTGTCCTGCTGATGTATGGTGGGTTGCAAAGGATGCCTGATGAACTGAACCAGCAATTGATGTAAAATTTCTTCGACGAAGCGCTTCAGCAGCTATTACACAAAGTTGAATCAGCAAAAATACTCCCAATATTTGATATCTGGGTCTTTGATTTGATGCTTTTCCAATGAACACATAACGAATGGCAGAAGTACGTTTGGATATATGATAATAAAGGCCTTCAAAGTAAAACAACATGAGGTTAGCACGAAGAACCAGCTGCAATAACTCACGAACAAAAGGGAGCATCGTGGGCCATTTCTGAACCATACGCAACCAAAATCCACAGAGCTTCTCCTTCAACCTCGTCATAGTTGAAGTAGACTGCCCTGAGGCAGACGAAGCAGCAAGTTCACTCCCCGAAGAAACCCGACTACTACTACTCCCGCGGGCACTTTCAACCAAAAATCYCNRCAYGACTAATACCTACGTAACTACGAACGAACGAGGTAATTAAGTACGTCCGGTTAAGGGAACACTAACTTACGTAACGTAAACTTAACGTTACCCGG
TARGET=948,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06078 Ps_contig_mira-and-tgicl-ass_franssen_41485
SEQUENCE=AGTAAAATGGGTTACTTTTACATTTTGTTTGGAGGTTTGAAAATATAAATCAAATTCAAACATATTTTACACGTATTTGTAATCTCGTTTTGTAATCCTGCCACAATATAAATAGGATTTTTCTTATTTTCTAATATAAAATTAAATTGTTTAAATGTTCATTTTGATACAATCCTCAAGATCAAGAAAATGTTGTCTTTCAAACTTTCATGCTATCTTCTTTCCATTTACAAAAGACTGCAGAGAGACAGATTCTAGAAGTCTGAGTAATAAATACAAACTAGACTTGAGTGTGTTATAGGGGTGCGGCAAAGAGGGCACTCTGGCTTCTCGTTGCACCATTCTGTGATACAGTTCCAGCAAAATACATGGCCACATGAAGTGGCTGTGGGGTGTTGACGATTACTCAGGCAAAGTGTGCATTTGCTAACACCACTGGTTGCATGAGGCTCAGAAGATGAAGATCCAGAGACCCAACTTCCTTCATCAGCTTCTGGAGATGCCAAATTACCTTCTTCGTTTAGAACAGGCAAACCGTGTCCTGCTGATGTATGGTGGGTTGCAAAGGATGCCTGATGAACTGAACCAGCAATTGATGTAAAATTTCTTCGACGAAGCGCTTCAGCAGCTATTACACAAAGTTGAATCAGCAAAAATACTCCCAATATTTGATATCTGGGTCTTTGATTTGATGCTTTTCCAATGAACACATAACGAATGGCAGAAGTACGTTTGGATATATGATAATAAAGGCCTTCAAAGTAAAACAACATGAGGTTAGCACGAAGAACCAGCTGCAATAACTCACGAACAAAAGGGAGCATCGTGGGCCATTTCTGAACCATACGCAACCAAAATCCACAGAGCTTCTCCTTCAACCTCGTCATAGTTGAAGTAGACTGCCCTGAGGCAGACGAAGCAGCAAGTTCACTCCCCGAAGAAACCCGACTACTACTACTCCCGCGGGCACTTTCAACCAAAAATCYCNRCAYGACTAATACCTACGTAACTACGAACGAACGAGGTAATTAAGTACGTCCGGTTAAGGGAACACTAACTTACGTAACGTAAACTTAACGTTACCCGG
TARGET=1012,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06079 Ps_contig_mira-and-tgicl-ass_franssen_41491
SEQUENCE=ATATGTCTCCGCCAGGTATCTGCGAAGGCCGTATACGAAGCCATTAACAAATACAAAGTGACTCATTTTTGTGCAGCGCCGGTCGTGCTTAATTCGATAATGAATGCCCCGGCTGAAGATACCATACTTCCTTTACCACATGTTGTAGATGTGAATACAGGTGGTGCACCTCCTCCTCCTTCTCTATTGTCCGGCATGTCCAAACTCGGGTTTCGCGTCATGCACAATTACGGTCTTTCAGAAACCTTTGGCCCGTCTGTCTACTGCGCGTGGAATCCAGAGTGGGATTCGCTTCCAGCGGAATCCCAAGCCAGACTCCACGCGAGACAAGGTATTCGCTACATAGCCTTGGAAAACCTCGAAGTAATGAACCCGAAAACAATGCAACCCGTTCCGGCCGATGGCAAAACCATCGGCGAGATCGTGATGCGTGGTAATGTCGTGATGAAAGGCTACTTAAAGAATCCTAAAGCTAATGAAGAGGCTTTTGCGAATGGATGGTTTCATACTGGTGATCTTGCTGTGAAACATGAAGATGGATATATAGAAATTAAAGATAGGTCAAAAAGATATTATTATTTCGGGTGGTRRAAAATATAAGTAGTGTGGAGATAGAGAATGCTCTATACGCACATCCTGCAATTAGTTGGGAAGACCTTACGTAAGGGTTGGGTTTGACCGTTACGGGACCAAGTA
TARGET=169,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06080 Ps_contig_mira-and-tgicl-ass_franssen_41501
SEQUENCE=ATCCTTTGGGAGCACTATTGGATTATTGACTATCCTGTCATATGTATCTTGAAGTGTGTCACCAAGAAAAGGGTATTCGCCCAATATCATACAGTATAAAGTAATTCCTACTGCCCATGTGTCTGAGGCTTTACCTCGGTAGGTAAGACCTAAAATACATTCAGGTGCAGTGAAAACAGGAGTTCCAGGTGATCGTCGAAGCACATCATTATCATCCTCAAAAGCCTGGCTGACACTGAAATCCCCTATCTTTACTGTACCATGACGAGTAATCAACAGATTATCAGGCTTGATATCCCCGTGAACTATATTATGAGCATGGAGATACATTAATCCAGAAACAATATCCCGCAAGTATCTCCTAGCAGTCTCTTCACCTAAGCTACAAGCTCGACCTGAACCCTCGCAAACCCATTTGTCTTCCACATATTCAAGTACCATGTAGAAGTGATCGGATTCTGGGTCATCAATCACCTCAATGAGATTGACTATATTAGGATGTTGGATCATTTTCATTATGAAAACCTCACGTAGAACATCGGTCATGGCAGTTTCTGAGGGTCCAACTCGAAGCTTCCGTAAATGGGACTTATGAAAGGCCTTAATTGCATAATGTTTTCCATCAGCCGAGCTTCGGTGAAGAGACACTTTCCCATAGCTACCCGAACCAATCTTATACTCACGAACATACTCATTTATCATCTTGTTTCCATTTTCATCCTCTGTGCGAAAAACTTTGTCAGTCTCTTTAACAGGAAACTGTCTACAAATCATCCCATTCTCTACTCTAAAAACCAAAATATCCTCCGATCGCTTAGGACGACTTTGCTCCTCACTATCATCTCCACTGGTGGTGCTAGTAACCTCGTCATTACAGAAAGACTCTTCGTCATCAAAATCATCATCATCATCATCCAACAACAGCTCTTGGGAGAGATTACTATTGTAGATGGCAGGTCCTTTAACCTTCTGCGTGTTCCGTCTCTTGAACGAAAACCCGAGA
TARGET=898,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4681
PRIMER_LEFT_PENALTY=0.163658
PRIMER_RIGHT_PENALTY=0.304436
PRIMER_LEFT_SEQUENCE=TTGCTCCTCACTATCATCTCCA
PRIMER_RIGHT_SEQUENCE=AACACGCAGAAGGTTAAAGGAC
PRIMER_LEFT=825,22
PRIMER_RIGHT=977,22
PRIMER_LEFT_TM=59.836
PRIMER_RIGHT_TM=59.696
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=153
PRIMER_PAIR_PENALTY_1=0.5078
PRIMER_LEFT_1_PENALTY=0.203324
PRIMER_RIGHT_1_PENALTY=0.304436
PRIMER_LEFT_1_SEQUENCE=TCTCCACTGGTGGTGCTAGTAA
PRIMER_RIGHT_1_SEQUENCE=AACACGCAGAAGGTTAAAGGAC
PRIMER_LEFT_1=841,22
PRIMER_RIGHT_1=977,22
PRIMER_LEFT_1_TM=59.797
PRIMER_RIGHT_1_TM=59.696
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.7000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=137
PRIMER_PAIR_PENALTY_2=0.5237
PRIMER_LEFT_2_PENALTY=0.163658
PRIMER_RIGHT_2_PENALTY=0.360050
PRIMER_LEFT_2_SEQUENCE=TTGCTCCTCACTATCATCTCCA
PRIMER_RIGHT_2_SEQUENCE=AGAAGGTTAAAGGACCTGCCAT
PRIMER_LEFT_2=825,22
PRIMER_RIGHT_2=970,22
PRIMER_LEFT_2_TM=59.836
PRIMER_RIGHT_2_TM=60.360
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.5237
PRIMER_LEFT_3_PENALTY=0.163658
PRIMER_RIGHT_3_PENALTY=0.360050
PRIMER_LEFT_3_SEQUENCE=TTGCTCCTCACTATCATCTCCA
PRIMER_RIGHT_3_SEQUENCE=AAGGTTAAAGGACCTGCCATCT
PRIMER_LEFT_3=825,22
PRIMER_RIGHT_3=968,22
PRIMER_LEFT_3_TM=59.836
PRIMER_RIGHT_3_TM=60.360
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=144
PRIMER_PAIR_PENALTY_4=0.5256
PRIMER_LEFT_4_PENALTY=0.221115
PRIMER_RIGHT_4_PENALTY=0.304436
PRIMER_LEFT_4_SEQUENCE=GGACGACTTTGCTCCTCACTAT
PRIMER_RIGHT_4_SEQUENCE=AACACGCAGAAGGTTAAAGGAC
PRIMER_LEFT_4=817,22
PRIMER_RIGHT_4=977,22
PRIMER_LEFT_4_TM=59.779
PRIMER_RIGHT_4_TM=59.696
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.3000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=161
=
PRIMER_SEQUENCE_ID=SSR_06081 Ps_contig_mira-and-tgicl-ass_franssen_41503
SEQUENCE=AGGGAGTAAGAACCCTAGCTAGCTAGATCTCCATTAAATAAATAAAATTTTCCATTACAAATTAAGTTGTTCCTTTCTACGATGTCCCACAGTTGTCCTATTGACACTGAAATCCATAATTAATTAATGTTCTCAAAATTACAGAAAGATAGAGAAAAAGGAACAAAATTAGGGTTTCTTACCAAGAGTGTGCTTGTTATTGTGCATCCAAACCTTAAGCACATTTCTCTTGATGTAATTCTCAGCACAGAATTGTTCAATTGCAGCTTCGTCTTCTTTCTGGATTCTCCATCCAATTCCCTCCGCAAATGCTAGCATCTTATCTTTCTGCTCCTGTGTGAACTTCGTCCTAAACCTTTTCTTCGTTCCACTCCCGCCGCCGCCGCTGCTGCTTGGAATTGACACGTCGTCGTCTTCTCTACTGAATCCTCCACTGGATGCGACAGGAGGAAGAGCTAACGGACGATGATGTGAAACCGGCGGTGGAGTTAATAATTGGTGGTATCCTGGTGGAGGAGGTAGCGCTGCACGGTGGTAGTAAGGAGATAACTGATGGTGGTGGTACTGAGTCTGAGAGTGAGACCGCGGTTGATGATTATGGGAAATGACCTCGCCGTCGATCTCTTTGCGGTGGAAGTTACGGTGACAGTTACAAGCTGCGCAAATTACGGCTTCCAGTGTTCCTTCTTCACCTGCAGCTAAAAACTCGCAACAACCGTCAACGGCGTGACCACCTATACTCACGGCGTGATTCTTTTGACATTCTCTGTATCTGACTGTCCCTTTGGGCCCGTTTTTCAGAACACCGCCTGTAGGAATGACTGCTGCTCCATCGGTTTCCATAGTCCCGCCGCTCATTTTAGACCGTACGCCGGAGTTTCCAAGCGAGTCGTAACCTGCAACCGGCGTAACCGGGAACCCCATTTCTTCTTCTTCCTCTTCCTCCTCCTCTTGATCTTCTTCGTGTTCGTCAAATTCCATTTCGCTTTTCACCAAATAAAAAAAAGTGTAAGACTTGAGAAATGAGGTGAGTCAGTTGAATGATGAAGTCATAATAATAATTTCACCGATCGGAGTT
TARGET=15,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06082 Ps_contig_mira-and-tgicl-ass_franssen_41503
SEQUENCE=AGGGAGTAAGAACCCTAGCTAGCTAGATCTCCATTAAATAAATAAAATTTTCCATTACAAATTAAGTTGTTCCTTTCTACGATGTCCCACAGTTGTCCTATTGACACTGAAATCCATAATTAATTAATGTTCTCAAAATTACAGAAAGATAGAGAAAAAGGAACAAAATTAGGGTTTCTTACCAAGAGTGTGCTTGTTATTGTGCATCCAAACCTTAAGCACATTTCTCTTGATGTAATTCTCAGCACAGAATTGTTCAATTGCAGCTTCGTCTTCTTTCTGGATTCTCCATCCAATTCCCTCCGCAAATGCTAGCATCTTATCTTTCTGCTCCTGTGTGAACTTCGTCCTAAACCTTTTCTTCGTTCCACTCCCGCCGCCGCCGCTGCTGCTTGGAATTGACACGTCGTCGTCTTCTCTACTGAATCCTCCACTGGATGCGACAGGAGGAAGAGCTAACGGACGATGATGTGAAACCGGCGGTGGAGTTAATAATTGGTGGTATCCTGGTGGAGGAGGTAGCGCTGCACGGTGGTAGTAAGGAGATAACTGATGGTGGTGGTACTGAGTCTGAGAGTGAGACCGCGGTTGATGATTATGGGAAATGACCTCGCCGTCGATCTCTTTGCGGTGGAAGTTACGGTGACAGTTACAAGCTGCGCAAATTACGGCTTCCAGTGTTCCTTCTTCACCTGCAGCTAAAAACTCGCAACAACCGTCAACGGCGTGACCACCTATACTCACGGCGTGATTCTTTTGACATTCTCTGTATCTGACTGTCCCTTTGGGCCCGTTTTTCAGAACACCGCCTGTAGGAATGACTGCTGCTCCATCGGTTTCCATAGTCCCGCCGCTCATTTTAGACCGTACGCCGGAGTTTCCAAGCGAGTCGTAACCTGCAACCGGCGTAACCGGGAACCCCATTTCTTCTTCTTCCTCTTCCTCCTCCTCTTGATCTTCTTCGTGTTCGTCAAATTCCATTTCGCTTTTCACCAAATAAAAAAAAGTGTAAGACTTGAGAAATGAGGTGAGTCAGTTGAATGATGAAGTCATAATAATAATTTCACCGATCGGAGTT
TARGET=35,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06083 Ps_contig_mira-and-tgicl-ass_franssen_41503
SEQUENCE=AGGGAGTAAGAACCCTAGCTAGCTAGATCTCCATTAAATAAATAAAATTTTCCATTACAAATTAAGTTGTTCCTTTCTACGATGTCCCACAGTTGTCCTATTGACACTGAAATCCATAATTAATTAATGTTCTCAAAATTACAGAAAGATAGAGAAAAAGGAACAAAATTAGGGTTTCTTACCAAGAGTGTGCTTGTTATTGTGCATCCAAACCTTAAGCACATTTCTCTTGATGTAATTCTCAGCACAGAATTGTTCAATTGCAGCTTCGTCTTCTTTCTGGATTCTCCATCCAATTCCCTCCGCAAATGCTAGCATCTTATCTTTCTGCTCCTGTGTGAACTTCGTCCTAAACCTTTTCTTCGTTCCACTCCCGCCGCCGCCGCTGCTGCTTGGAATTGACACGTCGTCGTCTTCTCTACTGAATCCTCCACTGGATGCGACAGGAGGAAGAGCTAACGGACGATGATGTGAAACCGGCGGTGGAGTTAATAATTGGTGGTATCCTGGTGGAGGAGGTAGCGCTGCACGGTGGTAGTAAGGAGATAACTGATGGTGGTGGTACTGAGTCTGAGAGTGAGACCGCGGTTGATGATTATGGGAAATGACCTCGCCGTCGATCTCTTTGCGGTGGAAGTTACGGTGACAGTTACAAGCTGCGCAAATTACGGCTTCCAGTGTTCCTTCTTCACCTGCAGCTAAAAACTCGCAACAACCGTCAACGGCGTGACCACCTATACTCACGGCGTGATTCTTTTGACATTCTCTGTATCTGACTGTCCCTTTGGGCCCGTTTTTCAGAACACCGCCTGTAGGAATGACTGCTGCTCCATCGGTTTCCATAGTCCCGCCGCTCATTTTAGACCGTACGCCGGAGTTTCCAAGCGAGTCGTAACCTGCAACCGGCGTAACCGGGAACCCCATTTCTTCTTCTTCCTCTTCCTCCTCCTCTTGATCTTCTTCGTGTTCGTCAAATTCCATTTCGCTTTTCACCAAATAAAAAAAAGTGTAAGACTTGAGAAATGAGGTGAGTCAGTTGAATGATGAAGTCATAATAATAATTTCACCGATCGGAGTT
TARGET=117,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0491
PRIMER_LEFT_PENALTY=0.030276
PRIMER_RIGHT_PENALTY=0.018835
PRIMER_LEFT_SEQUENCE=TTTCTACGATGTCCCACAGTTG
PRIMER_RIGHT_SEQUENCE=ATCCAGAAAGAAGACGAAGCTG
PRIMER_LEFT=73,22
PRIMER_RIGHT=284,22
PRIMER_LEFT_TM=60.030
PRIMER_RIGHT_TM=60.019
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.0702
PRIMER_LEFT_1_PENALTY=0.030276
PRIMER_RIGHT_1_PENALTY=0.039879
PRIMER_LEFT_1_SEQUENCE=TTTCTACGATGTCCCACAGTTG
PRIMER_RIGHT_1_SEQUENCE=GCACACTCTTGGTAAGAAACCC
PRIMER_LEFT_1=73,22
PRIMER_RIGHT_1=192,22
PRIMER_LEFT_1_TM=60.030
PRIMER_RIGHT_1_TM=60.040
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=0.0752
PRIMER_LEFT_2_PENALTY=0.035367
PRIMER_RIGHT_2_PENALTY=0.039879
PRIMER_LEFT_2_SEQUENCE=TCTACGATGTCCCACAGTTGTC
PRIMER_RIGHT_2_SEQUENCE=GCACACTCTTGGTAAGAAACCC
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=192,22
PRIMER_LEFT_2_TM=60.035
PRIMER_RIGHT_2_TM=60.040
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=118
PRIMER_PAIR_PENALTY_3=0.1487
PRIMER_LEFT_3_PENALTY=0.129818
PRIMER_RIGHT_3_PENALTY=0.018835
PRIMER_LEFT_3_SEQUENCE=GTTCCTTTCTACGATGTCCCAC
PRIMER_RIGHT_3_SEQUENCE=ATCCAGAAAGAAGACGAAGCTG
PRIMER_LEFT_3=68,22
PRIMER_RIGHT_3=284,22
PRIMER_LEFT_3_TM=59.870
PRIMER_RIGHT_3_TM=60.019
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=217
PRIMER_PAIR_PENALTY_4=0.1697
PRIMER_LEFT_4_PENALTY=0.129818
PRIMER_RIGHT_4_PENALTY=0.039879
PRIMER_LEFT_4_SEQUENCE=GTTCCTTTCTACGATGTCCCAC
PRIMER_RIGHT_4_SEQUENCE=GCACACTCTTGGTAAGAAACCC
PRIMER_LEFT_4=68,22
PRIMER_RIGHT_4=192,22
PRIMER_LEFT_4_TM=59.870
PRIMER_RIGHT_4_TM=60.040
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=125
=
PRIMER_SEQUENCE_ID=SSR_06084 Ps_contig_mira-and-tgicl-ass_franssen_41503
SEQUENCE=AGGGAGTAAGAACCCTAGCTAGCTAGATCTCCATTAAATAAATAAAATTTTCCATTACAAATTAAGTTGTTCCTTTCTACGATGTCCCACAGTTGTCCTATTGACACTGAAATCCATAATTAATTAATGTTCTCAAAATTACAGAAAGATAGAGAAAAAGGAACAAAATTAGGGTTTCTTACCAAGAGTGTGCTTGTTATTGTGCATCCAAACCTTAAGCACATTTCTCTTGATGTAATTCTCAGCACAGAATTGTTCAATTGCAGCTTCGTCTTCTTTCTGGATTCTCCATCCAATTCCCTCCGCAAATGCTAGCATCTTATCTTTCTGCTCCTGTGTGAACTTCGTCCTAAACCTTTTCTTCGTTCCACTCCCGCCGCCGCCGCTGCTGCTTGGAATTGACACGTCGTCGTCTTCTCTACTGAATCCTCCACTGGATGCGACAGGAGGAAGAGCTAACGGACGATGATGTGAAACCGGCGGTGGAGTTAATAATTGGTGGTATCCTGGTGGAGGAGGTAGCGCTGCACGGTGGTAGTAAGGAGATAACTGATGGTGGTGGTACTGAGTCTGAGAGTGAGACCGCGGTTGATGATTATGGGAAATGACCTCGCCGTCGATCTCTTTGCGGTGGAAGTTACGGTGACAGTTACAAGCTGCGCAAATTACGGCTTCCAGTGTTCCTTCTTCACCTGCAGCTAAAAACTCGCAACAACCGTCAACGGCGTGACCACCTATACTCACGGCGTGATTCTTTTGACATTCTCTGTATCTGACTGTCCCTTTGGGCCCGTTTTTCAGAACACCGCCTGTAGGAATGACTGCTGCTCCATCGGTTTCCATAGTCCCGCCGCTCATTTTAGACCGTACGCCGGAGTTTCCAAGCGAGTCGTAACCTGCAACCGGCGTAACCGGGAACCCCATTTCTTCTTCTTCCTCTTCCTCCTCCTCTTGATCTTCTTCGTGTTCGTCAAATTCCATTTCGCTTTTCACCAAATAAAAAAAAGTGTAAGACTTGAGAAATGAGGTGAGTCAGTTGAATGATGAAGTCATAATAATAATTTCACCGATCGGAGTT
TARGET=374,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1363
PRIMER_LEFT_PENALTY=0.018835
PRIMER_RIGHT_PENALTY=0.117452
PRIMER_LEFT_SEQUENCE=CAGCTTCGTCTTCTTTCTGGAT
PRIMER_RIGHT_SEQUENCE=ACCACCACCATCAGTTATCTCC
PRIMER_LEFT=263,22
PRIMER_RIGHT=562,22
PRIMER_LEFT_TM=60.019
PRIMER_RIGHT_TM=60.117
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=300
PRIMER_PAIR_PENALTY_1=0.1771
PRIMER_LEFT_1_PENALTY=0.018835
PRIMER_RIGHT_1_PENALTY=0.158297
PRIMER_LEFT_1_SEQUENCE=CAGCTTCGTCTTCTTTCTGGAT
PRIMER_RIGHT_1_SEQUENCE=ACATCATCGTCCGTTAGCTCTT
PRIMER_LEFT_1=263,22
PRIMER_RIGHT_1=471,22
PRIMER_LEFT_1_TM=60.019
PRIMER_RIGHT_1_TM=60.158
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.1835
PRIMER_LEFT_2_PENALTY=0.018835
PRIMER_RIGHT_2_PENALTY=0.164639
PRIMER_LEFT_2_SEQUENCE=CAGCTTCGTCTTCTTTCTGGAT
PRIMER_RIGHT_2_SEQUENCE=CCACCAGGATACCACCAATTAT
PRIMER_LEFT_2=263,22
PRIMER_RIGHT_2=512,22
PRIMER_LEFT_2_TM=60.019
PRIMER_RIGHT_2_TM=59.835
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=5.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.1860
PRIMER_LEFT_3_PENALTY=0.027715
PRIMER_RIGHT_3_PENALTY=0.158297
PRIMER_LEFT_3_SEQUENCE=AATTGCAGCTTCGTCTTCTTTC
PRIMER_RIGHT_3_SEQUENCE=ACATCATCGTCCGTTAGCTCTT
PRIMER_LEFT_3=258,22
PRIMER_RIGHT_3=471,22
PRIMER_LEFT_3_TM=60.028
PRIMER_RIGHT_3_TM=60.158
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.1924
PRIMER_LEFT_4_PENALTY=0.027715
PRIMER_RIGHT_4_PENALTY=0.164639
PRIMER_LEFT_4_SEQUENCE=AATTGCAGCTTCGTCTTCTTTC
PRIMER_RIGHT_4_SEQUENCE=CCACCAGGATACCACCAATTAT
PRIMER_LEFT_4=258,22
PRIMER_RIGHT_4=512,22
PRIMER_LEFT_4_TM=60.028
PRIMER_RIGHT_4_TM=59.835
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=5.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_06085 Ps_contig_mira-and-tgicl-ass_franssen_41503
SEQUENCE=AGGGAGTAAGAACCCTAGCTAGCTAGATCTCCATTAAATAAATAAAATTTTCCATTACAAATTAAGTTGTTCCTTTCTACGATGTCCCACAGTTGTCCTATTGACACTGAAATCCATAATTAATTAATGTTCTCAAAATTACAGAAAGATAGAGAAAAAGGAACAAAATTAGGGTTTCTTACCAAGAGTGTGCTTGTTATTGTGCATCCAAACCTTAAGCACATTTCTCTTGATGTAATTCTCAGCACAGAATTGTTCAATTGCAGCTTCGTCTTCTTTCTGGATTCTCCATCCAATTCCCTCCGCAAATGCTAGCATCTTATCTTTCTGCTCCTGTGTGAACTTCGTCCTAAACCTTTTCTTCGTTCCACTCCCGCCGCCGCCGCTGCTGCTTGGAATTGACACGTCGTCGTCTTCTCTACTGAATCCTCCACTGGATGCGACAGGAGGAAGAGCTAACGGACGATGATGTGAAACCGGCGGTGGAGTTAATAATTGGTGGTATCCTGGTGGAGGAGGTAGCGCTGCACGGTGGTAGTAAGGAGATAACTGATGGTGGTGGTACTGAGTCTGAGAGTGAGACCGCGGTTGATGATTATGGGAAATGACCTCGCCGTCGATCTCTTTGCGGTGGAAGTTACGGTGACAGTTACAAGCTGCGCAAATTACGGCTTCCAGTGTTCCTTCTTCACCTGCAGCTAAAAACTCGCAACAACCGTCAACGGCGTGACCACCTATACTCACGGCGTGATTCTTTTGACATTCTCTGTATCTGACTGTCCCTTTGGGCCCGTTTTTCAGAACACCGCCTGTAGGAATGACTGCTGCTCCATCGGTTTCCATAGTCCCGCCGCTCATTTTAGACCGTACGCCGGAGTTTCCAAGCGAGTCGTAACCTGCAACCGGCGTAACCGGGAACCCCATTTCTTCTTCTTCCTCTTCCTCCTCCTCTTGATCTTCTTCGTGTTCGTCAAATTCCATTTCGCTTTTCACCAAATAAAAAAAAGTGTAAGACTTGAGAAATGAGGTGAGTCAGTTGAATGATGAAGTCATAATAATAATTTCACCGATCGGAGTT
TARGET=925,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5198
PRIMER_LEFT_PENALTY=0.145769
PRIMER_RIGHT_PENALTY=0.373998
PRIMER_LEFT_SEQUENCE=CTTCCAGTGTTCCTTCTTCACC
PRIMER_RIGHT_SEQUENCE=CACGAAGAAGATCAAGAGGAGG
PRIMER_LEFT=671,22
PRIMER_RIGHT=965,22
PRIMER_LEFT_TM=60.146
PRIMER_RIGHT_TM=60.374
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=0.5524
PRIMER_LEFT_1_PENALTY=0.145769
PRIMER_RIGHT_1_PENALTY=0.406596
PRIMER_LEFT_1_SEQUENCE=CTTCCAGTGTTCCTTCTTCACC
PRIMER_RIGHT_1_SEQUENCE=CGAAGAAGATCAAGAGGAGGAG
PRIMER_LEFT_1=671,22
PRIMER_RIGHT_1=963,22
PRIMER_LEFT_1_TM=60.146
PRIMER_RIGHT_1_TM=59.593
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=293
PRIMER_PAIR_PENALTY_2=0.6955
PRIMER_LEFT_2_PENALTY=0.288917
PRIMER_RIGHT_2_PENALTY=0.406596
PRIMER_LEFT_2_SEQUENCE=GCTTCCAGTGTTCCTTCTTCAC
PRIMER_RIGHT_2_SEQUENCE=CGAAGAAGATCAAGAGGAGGAG
PRIMER_LEFT_2=670,22
PRIMER_RIGHT_2=963,22
PRIMER_LEFT_2_TM=60.289
PRIMER_RIGHT_2_TM=59.593
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=294
PRIMER_PAIR_PENALTY_3=0.8277
PRIMER_LEFT_3_PENALTY=0.288917
PRIMER_RIGHT_3_PENALTY=0.538752
PRIMER_LEFT_3_SEQUENCE=GCTTCCAGTGTTCCTTCTTCAC
PRIMER_RIGHT_3_SEQUENCE=ACGAAGAAGATCAAGAGGAGGA
PRIMER_LEFT_3=670,22
PRIMER_RIGHT_3=964,22
PRIMER_LEFT_3_TM=60.289
PRIMER_RIGHT_3_TM=59.461
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=1.0242
PRIMER_LEFT_4_PENALTY=0.145769
PRIMER_RIGHT_4_PENALTY=0.878426
PRIMER_LEFT_4_SEQUENCE=CTTCCAGTGTTCCTTCTTCACC
PRIMER_RIGHT_4_SEQUENCE=CGAACACGAAGAAGATCAAGAG
PRIMER_LEFT_4=671,22
PRIMER_RIGHT_4=969,22
PRIMER_LEFT_4_TM=60.146
PRIMER_RIGHT_4_TM=59.122
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_06086 Ps_contig_mira-and-tgicl-ass_franssen_41508
SEQUENCE=TACATAAACAAACTCAAAATTCAAACAGTAAATCCAAAACTAGGTCTAAAAAGTAAAAAACAACATTCATAAATATTAATAGAAAAGACAAAATTGTATAAGAAAAACTTCTCTGCAGCCAAAATTACTTGACAACCACAAAGCCATCCATTACCATCATCACCGATTTTAGCGTCTGTCTGTCTTTCCTTTAGGGCGCTTTCCGGCATACAAGTGCTTTGGTTTAAGATTAGGTATGACTCTATCGGCTTCTCCACGCTTGGCATTCTTGTTTCTCTTCTTGACAGAACTCCTTGCAAGACCAATTGCCTTGATCTTTTGAGCAGAATCCCTGAGGCCTTCTCCAGGCACAACTTCATGCGGGGGCCTAGAGACCGACCTGGAACGTGACATTGATCTACTCGTACGCTGCTTCTTGCTAGGTGTGTCATCATCAATATCCATCCCATCGTTTCTTCCTTCTGGTGATCTCTCCCTTTTCCGACCTTTCCTAGATGCAGATCTGCTACGCATTCTGTTAACAGCCAGAGAAGGATCAATCCCGAGAGAAGACAGCTGTCTTCCCATTCTTTCTGTTGTGAACTGATTGTCCTTGTCGAATTTCCTTGCAACGATTGGTCTGCTCTCTGCAGTGCTCTTTTTAATCCTATGTTGTTGGATTAACAAACTTTTCTTCCTCCTAATCTCAGTTAAAGCCTCTTGCTGTTCTGGAGTCAATTCAGTTCCGTCGATCTCAAAATCATCATCTTCAGCTTCTGCCTGTCTTAATCCTTCTTCTCTTTCCAACTCTTCAACCCTGTGTAGAATATCGGGATCAATGAAATCATACACATTGTGGCCATCCAAAATTTCTGGCAGTATATCTTCTTTCCATTCATCATCAGCCAAAATATAATGCTTTCTCAAGTTCATCGAGTATACACCAGCCCCACCATTTTCTTCCTCCAGATCCTTCTCTGTTTTTCTCTTCTCCTCAGCAGCTGCTTGCTTAGCTTTAGCTTCCAAGACTGCCGGGGGAATACACAGCGGCCTTTCCTTCTGGTCACGTGGCTTCGGAACTGCAACATGAAACCTGTTCAGACAGTCATTGATTTTCTTAGACTTCATCTTTATCTCCACCCTCTGATTCAACAATCTCTCACAAGCTGCATTTTTCACCGCGATCACGCCTTCTTCTGTCAACGCACTCATGGTCAACAACACATCCGCTTCTGTCCCCTCACCTCCTTGACCAATTGCAGTCTTCAACGCTTCAGCTTTCATCTCCGTCACCAACTTCATATCTTCCTCAGATAATCCATCCAACGGCTGCAAATCAGTCTTGTTGCACACAACAATCAGAGGCTTGTTCAAGAACAAAGACTTAATGCTCTGAAACAAAGCCGCCTGCTGAGCAATGGTGTAACCGCAAGACCCAGATATGTCCAAGAAGAACAATATCGCCGCTCTCAAATGTGCAAGAGCAGTGATACTGCACATCTCAATGNTATTCCGATCCTCAAACGGCCTGTCCAAAATCCCTGGCGTATCAATCACTTGATACCTCAGATACTTATAATCCGTATGACCCACAAAAAGAGACTTCGTAGTAAAAGCATACGGCTGCACATCAACATCCGCTCGAGTAATCTTATTAATAAAAGAACTCTTGCCAACATTCGGATAACCACAAATCAAAATCGTCCTCGTATTCGG
TARGET=174,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0542
PRIMER_LEFT_PENALTY=0.047267
PRIMER_RIGHT_PENALTY=0.006945
PRIMER_LEFT_SEQUENCE=AAAGCCATCCATTACCATCATC
PRIMER_RIGHT_SEQUENCE=TAGATCAATGTCACGTTCCAGG
PRIMER_LEFT=139,22
PRIMER_RIGHT=399,22
PRIMER_LEFT_TM=60.047
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.1047
PRIMER_LEFT_1_PENALTY=0.047267
PRIMER_RIGHT_1_PENALTY=0.057411
PRIMER_LEFT_1_SEQUENCE=AAAGCCATCCATTACCATCATC
PRIMER_RIGHT_1_SEQUENCE=ATGACACACCTAGCAAGAAGCA
PRIMER_LEFT_1=139,22
PRIMER_RIGHT_1=430,22
PRIMER_LEFT_1_TM=60.047
PRIMER_RIGHT_1_TM=59.943
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.1147
PRIMER_LEFT_2_PENALTY=0.047267
PRIMER_RIGHT_2_PENALTY=0.067430
PRIMER_LEFT_2_SEQUENCE=AAAGCCATCCATTACCATCATC
PRIMER_RIGHT_2_SEQUENCE=GCAGCGTACGAGTAGATCAATG
PRIMER_LEFT_2=139,22
PRIMER_RIGHT_2=411,22
PRIMER_LEFT_2_TM=60.047
PRIMER_RIGHT_2_TM=59.933
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.1489
PRIMER_LEFT_3_PENALTY=0.047267
PRIMER_RIGHT_3_PENALTY=0.101680
PRIMER_LEFT_3_SEQUENCE=AAAGCCATCCATTACCATCATC
PRIMER_RIGHT_3_SEQUENCE=AAGAGAAACAAGAATGCCAAGC
PRIMER_LEFT_3=139,22
PRIMER_RIGHT_3=278,22
PRIMER_LEFT_3_TM=60.047
PRIMER_RIGHT_3_TM=59.898
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=140
PRIMER_PAIR_PENALTY_4=0.1848
PRIMER_LEFT_4_PENALTY=0.047267
PRIMER_RIGHT_4_PENALTY=0.137550
PRIMER_LEFT_4_SEQUENCE=AAAGCCATCCATTACCATCATC
PRIMER_RIGHT_4_SEQUENCE=ATTCTGCTCAAAAGATCAAGGC
PRIMER_LEFT_4=139,22
PRIMER_RIGHT_4=328,22
PRIMER_LEFT_4_TM=60.047
PRIMER_RIGHT_4_TM=59.862
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=190
=
PRIMER_SEQUENCE_ID=SSR_06087 Ps_contig_mira-and-tgicl-ass_franssen_41517
SEQUENCE=CCATAAATGGATGATGGGACAAAAATACTTTTTTCAATATCCCAACTTAACTCTTGATGGCTAAAATCCTCTAGCATTCTCTTGTCTTATAATGACAATTTAAAATGAATAGATACTTCCAATCTCAATAAAAATACAAAGTCCCTAAATTTGATAGTGTACAAAATTTATAGTGTCTACATCTTGAAGTACTTGACATGGTCTAACTCGACTCCTCTGGTTGTTCATTAGCCATCGCAACCTGGTCGTTTAGTTATCTTCTTCGTCGTCGTCGTCGTCCTCATCGTCGTCGTCGTCATCGTCCTCTTCATCAAGGTCGTTAGCTTTAGGTAGTTTGTTAACTTCAATGGAATGTAGTAGCAGCTCATTAGCCTCTATTTTATCTTCTTCTGTTTTTGCTAAAGAAAGAAGGATATTTTTAGCTTTCTGATCAGGAACAAGTCCACTCAACTCCATTTCTTTAAACCAATGAACAGCACTGTCAAACTCTCCATTTTTTCCATATGCATCCATTATCGTCGTCAAAATTGTTTGATTGGCCTTGACACCTCGCGCAAGCATTTCCTCGTACTTTTCCATTACCTTCTCAATATCATTTTCCTTAGCATACCCTTTAAGTATGGTTCCATAGGTGACAACATTAGGCTCAAAGCCGTCTTGTATCAATCTTCTGAAGAACTTCTCGGCACCATCCATGTCAGAAGCATTGACGTATGCTAATAACATGGTGGTATATGAGCAAAGATCTGGCATGTACTTATCCCTTCTCATGGTCTTAAACACAGTCCGAGCTTGCTCTACCATTCCCGATATGGAAAATGCATCAAGTAAAATATTGTAAGCTTTTCGAGTTGGTCTGACACCTGCGTCAAGCATTTCCTCAAATACTGCTAATGCTTCGTCCTCCCTCCTGGCTTTCCCATAAGCATTGATGAGTAAGGCATAGCTAACCACGTCGGGTTTGAGATCAGCTCTTTGCATCTGATCATATATGTTTGAAACTTCCTTGTAATTTGTTTCAAATGACATCAAGCTATTATATGTTACAGTGGATCGTTCAATTCCCCGTTCAGCCATCAGTGTGAATGTGTCACGGGCCTTTTCATGACTTCCAGCCTTCTTATACATATAAATCATCATATTAAACATCTTCTGATCTGGTTTCAAAGGTGAATTTTCATCATTCAATAGCTTGTCAAATACTTCTTCCGCTTCCTTAAACTTGTTCCCCTCAACAAATGTTTTAAGTATAATCTGATATGTGAATGCAGAAGGCTCGGGGCCAAATGTTTGCATCCTACGAAAAATCGATTCAGCATTGTTGTATCTGCCTCCTTTTCCATATGCTTCCATAAGTGCCGTTTGAGACACTACATTTGGTGCATGACCACTCTTATTCATCAAGCCCAACACCCTCTCGGCACCATTGAAGTCTCCAACCTTTCCATAAGCCGTAATGAAGTCATGATGAAATCCATCTTACTAAA
TARGET=263,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3924
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.261998
PRIMER_LEFT_SEQUENCE=CTCCTCTGGTTGTTCATTAGCC
PRIMER_RIGHT_SEQUENCE=GCTAACGACCTTGATGAAGAGG
PRIMER_LEFT=211,22
PRIMER_RIGHT=323,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.262
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=113
PRIMER_PAIR_PENALTY_1=0.4775
PRIMER_LEFT_1_PENALTY=0.215550
PRIMER_RIGHT_1_PENALTY=0.261998
PRIMER_LEFT_1_SEQUENCE=AACTCGACTCCTCTGGTTGTTC
PRIMER_RIGHT_1_SEQUENCE=GCTAACGACCTTGATGAAGAGG
PRIMER_LEFT_1=204,22
PRIMER_RIGHT_1=323,22
PRIMER_LEFT_1_TM=59.784
PRIMER_RIGHT_1_TM=60.262
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=0.4852
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.354881
PRIMER_LEFT_2_SEQUENCE=CTCCTCTGGTTGTTCATTAGCC
PRIMER_RIGHT_2_SEQUENCE=GGAGTTGAGTGGACTTGTTCCT
PRIMER_LEFT_2=211,22
PRIMER_RIGHT_2=453,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=59.645
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=243
PRIMER_PAIR_PENALTY_3=0.5704
PRIMER_LEFT_3_PENALTY=0.215550
PRIMER_RIGHT_3_PENALTY=0.354881
PRIMER_LEFT_3_SEQUENCE=AACTCGACTCCTCTGGTTGTTC
PRIMER_RIGHT_3_SEQUENCE=GGAGTTGAGTGGACTTGTTCCT
PRIMER_LEFT_3=204,22
PRIMER_RIGHT_3=453,22
PRIMER_LEFT_3_TM=59.784
PRIMER_RIGHT_3_TM=59.645
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.6091
PRIMER_LEFT_4_PENALTY=0.347129
PRIMER_RIGHT_4_PENALTY=0.261998
PRIMER_LEFT_4_SEQUENCE=CCCAACTTAACTCTTGATGGCT
PRIMER_RIGHT_4_SEQUENCE=GCTAACGACCTTGATGAAGAGG
PRIMER_LEFT_4=40,22
PRIMER_RIGHT_4=323,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=60.262
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_06088 Ps_contig_mira-and-tgicl-ass_franssen_41517
SEQUENCE=CCATAAATGGATGATGGGACAAAAATACTTTTTTCAATATCCCAACTTAACTCTTGATGGCTAAAATCCTCTAGCATTCTCTTGTCTTATAATGACAATTTAAAATGAATAGATACTTCCAATCTCAATAAAAATACAAAGTCCCTAAATTTGATAGTGTACAAAATTTATAGTGTCTACATCTTGAAGTACTTGACATGGTCTAACTCGACTCCTCTGGTTGTTCATTAGCCATCGCAACCTGGTCGTTTAGTTATCTTCTTCGTCGTCGTCGTCGTCCTCATCGTCGTCGTCGTCATCGTCCTCTTCATCAAGGTCGTTAGCTTTAGGTAGTTTGTTAACTTCAATGGAATGTAGTAGCAGCTCATTAGCCTCTATTTTATCTTCTTCTGTTTTTGCTAAAGAAAGAAGGATATTTTTAGCTTTCTGATCAGGAACAAGTCCACTCAACTCCATTTCTTTAAACCAATGAACAGCACTGTCAAACTCTCCATTTTTTCCATATGCATCCATTATCGTCGTCAAAATTGTTTGATTGGCCTTGACACCTCGCGCAAGCATTTCCTCGTACTTTTCCATTACCTTCTCAATATCATTTTCCTTAGCATACCCTTTAAGTATGGTTCCATAGGTGACAACATTAGGCTCAAAGCCGTCTTGTATCAATCTTCTGAAGAACTTCTCGGCACCATCCATGTCAGAAGCATTGACGTATGCTAATAACATGGTGGTATATGAGCAAAGATCTGGCATGTACTTATCCCTTCTCATGGTCTTAAACACAGTCCGAGCTTGCTCTACCATTCCCGATATGGAAAATGCATCAAGTAAAATATTGTAAGCTTTTCGAGTTGGTCTGACACCTGCGTCAAGCATTTCCTCAAATACTGCTAATGCTTCGTCCTCCCTCCTGGCTTTCCCATAAGCATTGATGAGTAAGGCATAGCTAACCACGTCGGGTTTGAGATCAGCTCTTTGCATCTGATCATATATGTTTGAAACTTCCTTGTAATTTGTTTCAAATGACATCAAGCTATTATATGTTACAGTGGATCGTTCAATTCCCCGTTCAGCCATCAGTGTGAATGTGTCACGGGCCTTTTCATGACTTCCAGCCTTCTTATACATATAAATCATCATATTAAACATCTTCTGATCTGGTTTCAAAGGTGAATTTTCATCATTCAATAGCTTGTCAAATACTTCTTCCGCTTCCTTAAACTTGTTCCCCTCAACAAATGTTTTAAGTATAATCTGATATGTGAATGCAGAAGGCTCGGGGCCAAATGTTTGCATCCTACGAAAAATCGATTCAGCATTGTTGTATCTGCCTCCTTTTCCATATGCTTCCATAAGTGCCGTTTGAGACACTACATTTGGTGCATGACCACTCTTATTCATCAAGCCCAACACCCTCTCGGCACCATTGAAGTCTCCAACCTTTCCATAAGCCGTAATGAAGTCATGATGAAATCCATCTTACTAAA
TARGET=284,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3924
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.261998
PRIMER_LEFT_SEQUENCE=CTCCTCTGGTTGTTCATTAGCC
PRIMER_RIGHT_SEQUENCE=GCTAACGACCTTGATGAAGAGG
PRIMER_LEFT=211,22
PRIMER_RIGHT=323,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=60.262
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=113
PRIMER_PAIR_PENALTY_1=0.4775
PRIMER_LEFT_1_PENALTY=0.215550
PRIMER_RIGHT_1_PENALTY=0.261998
PRIMER_LEFT_1_SEQUENCE=AACTCGACTCCTCTGGTTGTTC
PRIMER_RIGHT_1_SEQUENCE=GCTAACGACCTTGATGAAGAGG
PRIMER_LEFT_1=204,22
PRIMER_RIGHT_1=323,22
PRIMER_LEFT_1_TM=59.784
PRIMER_RIGHT_1_TM=60.262
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=0.4852
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.354881
PRIMER_LEFT_2_SEQUENCE=CTCCTCTGGTTGTTCATTAGCC
PRIMER_RIGHT_2_SEQUENCE=GGAGTTGAGTGGACTTGTTCCT
PRIMER_LEFT_2=211,22
PRIMER_RIGHT_2=453,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=59.645
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=243
PRIMER_PAIR_PENALTY_3=0.5704
PRIMER_LEFT_3_PENALTY=0.215550
PRIMER_RIGHT_3_PENALTY=0.354881
PRIMER_LEFT_3_SEQUENCE=AACTCGACTCCTCTGGTTGTTC
PRIMER_RIGHT_3_SEQUENCE=GGAGTTGAGTGGACTTGTTCCT
PRIMER_LEFT_3=204,22
PRIMER_RIGHT_3=453,22
PRIMER_LEFT_3_TM=59.784
PRIMER_RIGHT_3_TM=59.645
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=8.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=250
PRIMER_PAIR_PENALTY_4=0.6091
PRIMER_LEFT_4_PENALTY=0.347129
PRIMER_RIGHT_4_PENALTY=0.261998
PRIMER_LEFT_4_SEQUENCE=CCCAACTTAACTCTTGATGGCT
PRIMER_RIGHT_4_SEQUENCE=GCTAACGACCTTGATGAAGAGG
PRIMER_LEFT_4=40,22
PRIMER_RIGHT_4=323,22
PRIMER_LEFT_4_TM=59.653
PRIMER_RIGHT_4_TM=60.262
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_06089 Ps_contig_mira-and-tgicl-ass_franssen_41554
SEQUENCE=TACTTTAAAAAAATTAAATTAAATTTTAGTTATTTTGATTATTTTCAGGATTATGGTCTCCAGTTATTTCTATACCGCACACCATACTTGGTAGACCTAGACCGTGAGAACGTTGGGAATGTTACTAAAATTTAAGACTCAAATAAAGAGTGGTGATGCTTGGAGAGGAATGGATGTACTGATCTTACAACACGGTGGACTAGTTTGGGTCGGACCCCAACCGACCCGGACAACCGCAACCAAACCAGTGGGTAATTTAATTATTACGAAAGGGAGGGGTGGA
TARGET=11,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06090 Ps_contig_mira-and-tgicl-ass_franssen_41568
SEQUENCE=GTCGGGGNATCTTATCGAACCTACTTCATTCTTCTAGCTAGGNNTTCTTCTTCTCCTCCTTTTCGCTCTTCTTTCTTCAATCCAATTCCAATGGCCGACGAATCCCAATACGACACCACTCCACCCTCCCTCAAACGCAAGTACGATGAACAACCTCCTCACTCTAGACCCACCGGTTTCTCCGACGGACCTCCTCCTCCTTCCTACAACAACGTCCCTCCCCCCGCCACCACCGAGGTCGAACTCATGAAACAACGCGCCCAAGAAGTTGCCGCTCGTCTCCTCAGCGGCTCCGATATCGCTAAGCGTACCAAATTCGATGTCGTTACTCCCACCCCTTACGAATCCACCGATTTTAAGAATCAATATTCAGCTCCTTCGAGTATTCCTTCGTATACACATCAAGGTTCAAGCAAGAAGATTGAAATCCCTAATGGGAGGGTTGGTGTCATCATTGGAAAAGGTGGAGAAACCATCAAGTACCTTCAATTACAATCTGGTGCAAAAATCCAAGTCACTCGTGATATGGATGCTGACCCTAATTCTCCAAATAGATTGGTGGAGCTTACGGGAACTTCTGATGCTATTGCTACTGCTGAGAAACTAATCAATGAAGTTCTTGCTGAGGCTGAATCTGGAGGTAATGGCCTTGTTACTCGGAGAATGACTGGACAAGGAGGCGCTGATGAATTTTCGATGAAGATCCCTAATAACAAGGTTGGCCTTATCATTGGTAAAGGTGGAGAAACAATAAAGAGTATGCAAGCTACAACTGGAGCTAGAATTCAGGTGATTCCTCTTCATCTTCCCCCAGGTGACACCTCCACTGAAAGAACATTAAAAATTGAAGGGACCTCTGAACAAATTGATTCTGCAAAACAATTGGTCGATTCAATCCTCAGTGGCGAGAATCGACTTAGAAATCCATCAATGTCTGGTGGTTACTCTCAGCAAGGTTACCAAGCTCGGCCGCCCTCTAGCTGGGCTCCAC
TARGET=190,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1445
PRIMER_LEFT_PENALTY=0.034102
PRIMER_RIGHT_PENALTY=0.110393
PRIMER_LEFT_SEQUENCE=TTCGCTCTTCTTTCTTCAATCC
PRIMER_RIGHT_SEQUENCE=TAACGACATCGAATTTGGTACG
PRIMER_LEFT=61,22
PRIMER_RIGHT=327,22
PRIMER_LEFT_TM=59.966
PRIMER_RIGHT_TM=59.890
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.2326
PRIMER_LEFT_1_PENALTY=0.122257
PRIMER_RIGHT_1_PENALTY=0.110393
PRIMER_LEFT_1_SEQUENCE=CCTTTTCGCTCTTCTTTCTTCA
PRIMER_RIGHT_1_SEQUENCE=TAACGACATCGAATTTGGTACG
PRIMER_LEFT_1=57,22
PRIMER_RIGHT_1=327,22
PRIMER_LEFT_1_TM=60.122
PRIMER_RIGHT_1_TM=59.890
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=271
PRIMER_PAIR_PENALTY_2=0.3808
PRIMER_LEFT_2_PENALTY=0.187326
PRIMER_RIGHT_2_PENALTY=0.193502
PRIMER_LEFT_2_SEQUENCE=CTCAAACGCAAGTACGATGAAC
PRIMER_RIGHT_2_SEQUENCE=TAAAATCGGTGGATTCGTAAGG
PRIMER_LEFT_2=129,22
PRIMER_RIGHT_2=357,22
PRIMER_LEFT_2_TM=59.813
PRIMER_RIGHT_2_TM=60.194
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=229
PRIMER_PAIR_PENALTY_3=0.4072
PRIMER_LEFT_3_PENALTY=0.187326
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=CTCAAACGCAAGTACGATGAAC
PRIMER_RIGHT_3_SEQUENCE=TCTTGCTTGAACCTTGATGTGT
PRIMER_LEFT_3=129,22
PRIMER_RIGHT_3=417,22
PRIMER_LEFT_3_TM=59.813
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=0.4710
PRIMER_LEFT_4_PENALTY=0.187326
PRIMER_RIGHT_4_PENALTY=0.283676
PRIMER_LEFT_4_SEQUENCE=CTCAAACGCAAGTACGATGAAC
PRIMER_RIGHT_4_SEQUENCE=TTCTTGCTTGAACCTTGATGTG
PRIMER_LEFT_4=129,22
PRIMER_RIGHT_4=418,22
PRIMER_LEFT_4_TM=59.813
PRIMER_RIGHT_4_TM=60.284
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_06091 Ps_contig_mira-and-tgicl-ass_franssen_41571
SEQUENCE=GAGTACATTGAGACAAAGACAGAAAATTGGTAAGCTAAGCTATACATTTGACCATTCTGTGCTAGCACAAGTTCTCTCAATCACCTTTGTAATCTGTTCTNNTGAGAGTTTCTAGATGGAGAACTTGTTTTCTCTAGAAGAACTAATGTCCAAAGAAGACTAAGCCTACTAATTTTTTTCGTAAAAAGAAGCTCATAAATTTAACGCGATCCTGATTTCGCCATGTCCCTCTTCATGCAAACATGCTCCCTCTCTAGATCATTTAGTCTCATCCGTAGTCGAGCCAATTCGAGTTTTAACTCACGATTCTCGCGCCTCAATGATGCGTAGTTATCGCGTGGAGACATTGCTGCACTTAGTGCACCACTACTTATGCGCCACGACGCGTGCATTGGCTTGATGTCATCGTCCCCGTTGGTCGTGGACAACGAGTTTCTTAGCTTTAGTTGTTCGAAATATAGAACTTGCACTATTGATTGTAGAGGAAGGCGCTCGTTTTGCGCAGCRTGCGCTCCAGCTTCTTGAGTAAGTCTTTAGAAATCGTATTTAATTGCTATTTACGTTTCCTTCCTTCCTACCAAGTTCTAGTAACTTAACC
TARGET=564,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06092 Ps_contig_mira-and-tgicl-ass_franssen_41575
SEQUENCE=TCGGGGATCCTCATCCACATGCTGCAAACAGAAAGTGACCAACACATATGAGAAGTCAAGAATCTAAGCCGGATAAGCGTCGCAGCTCGTGCTTCTTTGAAAACTGGCGTATCATCGAGTGGATTCTGGTGTGGTGGTTCAAGAAGTTTCACCATCGCGTTTTCCATGTGCGTATTCTTCTTCGATTTGCACAAAGCATATGAGCCGGAAGCGGATCTAGATTCACAACGCAGCTTCAACAAAATGTCATCAAATCGAGTTCAATTGTCGTCTTCACCAAATCAAGATTTGTAAGATTCAACGTCGAAACTAAAATCTTTGAACTGCCTGCGACTCGCGTGCAAAATTGAGAATCACTGCGTATGAATTGACAATTGAAGCCAAAATAGGAACACTTACCGGATTGAAACTTTGCTTGTGAATAGGTACGAGTCCTCTTCTCTGATTCTGATCCGATTCGTCTTTGATTTCACGCTTCTTCTTCTTCACTTCGACCTTTCTTCTGACTCTGCTCCTCTGAACTTTTTGGCTGCGTGAGCGATGATGAACCTGCGTCTGTGGATTGAGGAGAATATGAAGAATACGCAAGTGGAAAAAATGCAGACTCTCGAAGTGAAAAATGAATCCTCTTCATTGAAGATAGGGCCTTGGCTTATTGTCTTTGAATAGTGGAAATTTGGTTGGTTGCTCTTGGCAATTGTTACTTGGAAGGGATTTGAATTATGCATTGATTGTCTACTTTTGTAATGAATAGTAAAATATTTAGGGTTAGGTTAATGTAATTTGATTGAGTAATAAAAGT
TARGET=475,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2035
PRIMER_LEFT_PENALTY=0.015253
PRIMER_RIGHT_PENALTY=0.188254
PRIMER_LEFT_SEQUENCE=GTTCAATTGTCGTCTTCACCAA
PRIMER_RIGHT_SEQUENCE=AGCAGAGTCAGAAGAAAGGTCG
PRIMER_LEFT=258,22
PRIMER_RIGHT=512,22
PRIMER_LEFT_TM=60.015
PRIMER_RIGHT_TM=60.188
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=255
PRIMER_PAIR_PENALTY_1=0.2897
PRIMER_LEFT_1_PENALTY=0.101479
PRIMER_RIGHT_1_PENALTY=0.188254
PRIMER_LEFT_1_SEQUENCE=GCCAAAATAGGAACACTTACCG
PRIMER_RIGHT_1_SEQUENCE=AGCAGAGTCAGAAGAAAGGTCG
PRIMER_LEFT_1=379,22
PRIMER_RIGHT_1=512,22
PRIMER_LEFT_1_TM=59.899
PRIMER_RIGHT_1_TM=60.188
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.9000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=134
PRIMER_PAIR_PENALTY_2=0.3168
PRIMER_LEFT_2_PENALTY=0.128591
PRIMER_RIGHT_2_PENALTY=0.188254
PRIMER_LEFT_2_SEQUENCE=AGGAACACTTACCGGATTGAAA
PRIMER_RIGHT_2_SEQUENCE=AGCAGAGTCAGAAGAAAGGTCG
PRIMER_LEFT_2=387,22
PRIMER_RIGHT_2=512,22
PRIMER_LEFT_2_TM=59.871
PRIMER_RIGHT_2_TM=60.188
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=126
PRIMER_PAIR_PENALTY_3=0.3348
PRIMER_LEFT_3_PENALTY=0.146525
PRIMER_RIGHT_3_PENALTY=0.188254
PRIMER_LEFT_3_SEQUENCE=TTGTCGTCTTCACCAAATCAAG
PRIMER_RIGHT_3_SEQUENCE=AGCAGAGTCAGAAGAAAGGTCG
PRIMER_LEFT_3=264,22
PRIMER_RIGHT_3=512,22
PRIMER_LEFT_3_TM=60.147
PRIMER_RIGHT_3_TM=60.188
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=249
PRIMER_PAIR_PENALTY_4=0.3820
PRIMER_LEFT_4_PENALTY=0.084327
PRIMER_RIGHT_4_PENALTY=0.297699
PRIMER_LEFT_4_SEQUENCE=GATCCGATTCGTCTTTGATTTC
PRIMER_RIGHT_4_SEQUENCE=CAAGTAACAATTGCCAAGAGCA
PRIMER_LEFT_4=449,22
PRIMER_RIGHT_4=706,22
PRIMER_LEFT_4_TM=59.916
PRIMER_RIGHT_4_TM=60.298
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_06093 Ps_contig_mira-and-tgicl-ass_franssen_41577
SEQUENCE=GTAAAGAAGGGGGTTAAAGGGAAGTTAAGTAGAAAGACAAATACTTGTTTCTAGAAAAGGGAAGTTGACCCTGTTATCTCCTCTGATGACATGAAGAATGCACCCACCACTGAAGTACCATGCCCAAATTGCCGCCATGATAAAGCTGCTTATAAGGAGGTTCAGACTAGATCAGCTGATGAGCCTGCGACATTGTTTTTCAAATGCTTGAATGTAAAGTGTGGGCACAATTGGAGAGAGGGTTGATATGCAATATATTTTTTGTCATGCCATAAAGATATAGAAGCTTTTCATGCTGTTACTGATTAAAATTATGTGACAGATAAGAGTGTTTTATTTTATTTTACTTTTTATCAAGTAATTGTTACAAAAATGACATTGGCTATAGAATTTTATAATCGTAATTGTGAAGTGTTTTTTGATGAACTCTTTTAGCAATCCTAACTATACATA
TARGET=332,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06094 Ps_contig_mira-and-tgicl-ass_franssen_41584
SEQUENCE=GGATAGGTGGTGGATTGGAACTGCCCCCAAGACGGAGGGATGGCCAGGACTATGCTGCTGCTGCTACTGGAGGCGGCAGTGGAACAGGGTCAGGTGGTAGGTGGCAAGACTTGTCCCATTCTCAGCCACGGCAAGGGAGTGGACGTGCAGGGTATGGTAATGCTGGTGGAAGGCCATTGGCATATGGTCAAGCAGCTAGGAGTGGCTATTCAAGGGATCGAACGGGTCGTGGAACAGGTGGTGGTTATCAGAGTTCAGGGAGGGCTACTCAAGGGGGTTCAGGACTGAGGGGATCCCAGGGCGATGTATCTACTCGAATGGTTAGCCTTAAGGGAGTTCGTGCTTAAAGAAGTTTACTAGAGATGGCATGTGATACAAGTTTTCTGCATCTTAAATTTGCTCATGCATGTCAGTTTTGATAAATTGACTATAGCACTAAAAGCATTATTGTTTTCATTATTGTCCTTGTTTTTATTTTTTGATGCTATTTATACTAAAGTTCCAATRAGRTTATTTTCGTTGGGGGTTTTATGTTTTCTTTTTATAACCATTTGGTTTGGTTGTTTTTATGTTTTCTTTTTANAAGCTATTTAGTTTGGGGTTTAATTAAAGTTTTAAAAAAAACCTT
TARGET=53,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06095 Ps_contig_mira-and-tgicl-ass_franssen_41585
SEQUENCE=AANNTCAATAGAAATGTTACGATTGATGAGTGGTTAATGTCAAACAAACAATAACAATAGATATATCAATATCCAAAGGAGAAACTTCTAACAAACAAACAAATGATTCAGGCGGATGTTCATCCGATCTCTTACTAATTTAAGAATACATGAAGAGAGGGCAAACTTTGTTTTAGAAGAGAAAGATACAGCACCAACACACCTCTCCAAACTTAGTATTTAATACTAATTACAACTTAATCTAATTTAGATCCACCCTTATCGGAACCCTTCCTTGTTGTTAGCAGATCCCAAAGAAGAAGTATCAAAGAATATAGTTAAAAACTTGTTCTATATAATCTCCTCTAATTAATTAGGACCCCAACAACTCAAGTACATGACCGTCCTCAAAGATTCTTCTGATTGCTTTGTCATCTTTGTTTGTCTTTTAGAATTAGAAACCATAGTTGAAGAAGTGGAGGATGAGAGTTTCTTTGCTTGAGAGTACGATCTGACATTGTTTTTAACATGATTTTGAAGTGATTTTAGTGTATGATTCCATCTGCATATACCTTGATCCTTTAAAGCTTCCACCACTGCAACACTGGCTGCTGCCGCCCATAGCTCTACTTGTTGAACTCATCTTCTTCTGTGTTAATAATTCTTCAAAGTGTATTTTGTGGTCTTTTTAGATGACAAGAATAAAATGGTATTACTTAGTTTTGTTAGTTAG
TARGET=90,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.9703
PRIMER_LEFT_PENALTY=2.908354
PRIMER_RIGHT_PENALTY=0.061961
PRIMER_LEFT_SEQUENCE=CGATTGATGAGTGGTTAATGTC
PRIMER_RIGHT_SEQUENCE=AAGTTTGGAGAGGTGTGTTGGT
PRIMER_LEFT=19,22
PRIMER_RIGHT=213,22
PRIMER_LEFT_TM=57.092
PRIMER_RIGHT_TM=59.938
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=195
PRIMER_PAIR_PENALTY_1=3.0729
PRIMER_LEFT_1_PENALTY=2.908354
PRIMER_RIGHT_1_PENALTY=0.164560
PRIMER_LEFT_1_SEQUENCE=CGATTGATGAGTGGTTAATGTC
PRIMER_RIGHT_1_SEQUENCE=TCTGCTAACAACAAGGAAGGGT
PRIMER_LEFT_1=19,22
PRIMER_RIGHT_1=287,22
PRIMER_LEFT_1_TM=57.092
PRIMER_RIGHT_1_TM=60.165
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=269
PRIMER_PAIR_PENALTY_2=3.1059
PRIMER_LEFT_2_PENALTY=2.908354
PRIMER_RIGHT_2_PENALTY=0.197549
PRIMER_LEFT_2_SEQUENCE=CGATTGATGAGTGGTTAATGTC
PRIMER_RIGHT_2_SEQUENCE=TTCCGATAAGGGTGGATCTAAA
PRIMER_LEFT_2=19,22
PRIMER_RIGHT_2=266,22
PRIMER_LEFT_2_TM=57.092
PRIMER_RIGHT_2_TM=59.802
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=248
PRIMER_PAIR_PENALTY_3=3.1618
PRIMER_LEFT_3_PENALTY=2.908354
PRIMER_RIGHT_3_PENALTY=0.253482
PRIMER_LEFT_3_SEQUENCE=CGATTGATGAGTGGTTAATGTC
PRIMER_RIGHT_3_SEQUENCE=TCTTTGGGATCTGCTAACAACA
PRIMER_LEFT_3=19,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=57.092
PRIMER_RIGHT_3_TM=59.747
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=3.2181
PRIMER_LEFT_4_PENALTY=2.908354
PRIMER_RIGHT_4_PENALTY=0.309780
PRIMER_LEFT_4_SEQUENCE=CGATTGATGAGTGGTTAATGTC
PRIMER_RIGHT_4_SEQUENCE=CTGCTAACAACAAGGAAGGGTT
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=286,22
PRIMER_LEFT_4_TM=57.092
PRIMER_RIGHT_4_TM=59.690
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=268
=
PRIMER_SEQUENCE_ID=SSR_06096 Ps_contig_mira-and-tgicl-ass_franssen_41587
SEQUENCE=TAAGGAGAAGGGAAAGTTTAAGCNCAAAGAATGTCGAGCATGAGGATAGTCTCTACATGCAACCTGACCCTTCTCTCCAACTACAACCAACTCATCTGACCCAGTTGCATCCTCATCATCAACAGAAGTGACACCATTTTCAGGGTCACCTTCCAGAACCAAACAATCATCATCATCATCACCATCATCAACAACTTTCACAGGCAAAGTTGAAGACTTTGACTTCAACTCAGGTGGTTTGTTCTCATGAAGGAAGACAACATCAGAATCACCATCAGAATCAGATTCATTTCCAGAATTGAAGAGCAACTCTTCAATCCATCCCAAATCAGTATTTTTGGACCCTTCTTTCAAATCCTCTTCCTC
TARGET=166,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1524
PRIMER_LEFT_PENALTY=0.099438
PRIMER_RIGHT_PENALTY=0.053000
PRIMER_LEFT_SEQUENCE=AACCAACTCATCTGACCCAGTT
PRIMER_RIGHT_SEQUENCE=AGAGGATTTGAAAGAAGGGTCC
PRIMER_LEFT=84,22
PRIMER_RIGHT=360,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=59.947
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=277
PRIMER_PAIR_PENALTY_1=0.1661
PRIMER_LEFT_1_PENALTY=0.017590
PRIMER_RIGHT_1_PENALTY=0.148509
PRIMER_LEFT_1_SEQUENCE=GGAGAAGGGAAAGTTTAAGCNC
PRIMER_RIGHT_1_SEQUENCE=TCATGAGAACAAACCACCTGAG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=249,22
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=60.149
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.50
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=9.5250
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=0.2479
PRIMER_LEFT_2_PENALTY=0.099438
PRIMER_RIGHT_2_PENALTY=0.148509
PRIMER_LEFT_2_SEQUENCE=AACCAACTCATCTGACCCAGTT
PRIMER_RIGHT_2_SEQUENCE=TCATGAGAACAAACCACCTGAG
PRIMER_LEFT_2=84,22
PRIMER_RIGHT_2=249,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=0.2511
PRIMER_LEFT_3_PENALTY=0.102638
PRIMER_RIGHT_3_PENALTY=0.148509
PRIMER_LEFT_3_SEQUENCE=AAAGAATGTCGAGCATGAGGAT
PRIMER_RIGHT_3_SEQUENCE=TCATGAGAACAAACCACCTGAG
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=249,22
PRIMER_LEFT_3_TM=60.103
PRIMER_RIGHT_3_TM=60.149
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.8000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=0.2864
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.186949
PRIMER_LEFT_4_SEQUENCE=AACCAACTCATCTGACCCAGTT
PRIMER_RIGHT_4_SEQUENCE=ACTGATTTGGGATGGATTGAAG
PRIMER_LEFT_4=84,22
PRIMER_RIGHT_4=332,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=60.187
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_06097 Ps_contig_mira-and-tgicl-ass_franssen_41588
SEQUENCE=GAGACAAGACCGTTTGTTTGTAATGTTTATGTTTATTTACTTCTCACACTTTCTTCTNTRCGGGGAATCCCAATTTCCCAAACACTACACAACACAACACAACAACCTTTTCTTTTCTTGTCTTCTTTCTTCTTCTTCTTCTCTCATTCCATGGAAACTCCTCATACTGTGGTAGTGAACCGGATCGAACCGGGTTCAAAGGAGAACGGTTTAAAAGCCGAGATTGATACTTCGGCGCCGTTTGAGTCTGTGAAAGAAGCCGTTACGAGATTCGGCGGTGTTGGTTACTGGAAACCTACTAACACGGTTAACCTCATCACTCCTCAGCAGCAAAGCACAAGCGAGCTTGATAACCGTTAAATTGGAAGAACAAACAGCGGGATTGGAGAAAGAGCTTATACTCAAAGAAAGGGAAACTCTTGATGTCTTGAAAGAATTGGAAAGTACGAAAAAACTTGTCGAAAATTTGAAATCAAAGCTTCATGCAGAAGAATCTGAAACAAATTTGAATATTAAGATGAATTTATGCGACAAAAAGGAGAATGAGAAAGAAGAAGAAAAGGAAAACCAAGTGAGTCGTGTTCCTTACAATTCAACATCACCTGGTTTAATATTAAAGGAATTGAAGCAGGCGAAATTGAATCTAAACCGGTCTACAAACGACATCGCTGATGTTCGAGTTTCCGTTGAATCGCTCAACAAGAAACTAGAGAAGGAAAGAATCTCTCTCGAGAAAACCCGCGCGAGGTTAACTCAGAATTCTTCGAAAATAACTTATCTAGAGGAAGAGCTAAACCAAACAAGACTTAAGCTGCAAGTAGTGAAAGAAGCTGAAATCGAATGTGGTTCTTCGGATGAACCTTCTGATGTTACGAAAGAGCTTCAACGGTTGAGTTCTGAGGCCGAACGGTTCAAGAAAATGGGAGAAGCTGCGAAATCGGAAGTCACGAGAGCAATGTCTGAGATTGAACAGACGAAAGCTATGATAAGAACATCCGAGATAAGGTTGGTGGCTGCTCGAAAAATGAAGGAAGCTGCTAAAGCGGCCGAAGCTGCTGCAATGGCCGAGATAAAAGCGTTATCTTATCACGATGGAGATTGTATGCAAAAGCGCGACGGAGTTACTCTAACCTTCGAAGAGTATAATGCTCTAACGTGCAAAGCTCGTGGCGCCGAGGAACAATCTAAGAAGAGAGTTCTTGACGCCATGTCTCAAGCTGAAGAAGCNAANTTTATCGAAAARGTGGTAACGGTTTTAAAAGGTAGGG
TARGET=87,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06098 Ps_contig_mira-and-tgicl-ass_franssen_41588
SEQUENCE=GAGACAAGACCGTTTGTTTGTAATGTTTATGTTTATTTACTTCTCACACTTTCTTCTNTRCGGGGAATCCCAATTTCCCAAACACTACACAACACAACACAACAACCTTTTCTTTTCTTGTCTTCTTTCTTCTTCTTCTTCTCTCATTCCATGGAAACTCCTCATACTGTGGTAGTGAACCGGATCGAACCGGGTTCAAAGGAGAACGGTTTAAAAGCCGAGATTGATACTTCGGCGCCGTTTGAGTCTGTGAAAGAAGCCGTTACGAGATTCGGCGGTGTTGGTTACTGGAAACCTACTAACACGGTTAACCTCATCACTCCTCAGCAGCAAAGCACAAGCGAGCTTGATAACCGTTAAATTGGAAGAACAAACAGCGGGATTGGAGAAAGAGCTTATACTCAAAGAAAGGGAAACTCTTGATGTCTTGAAAGAATTGGAAAGTACGAAAAAACTTGTCGAAAATTTGAAATCAAAGCTTCATGCAGAAGAATCTGAAACAAATTTGAATATTAAGATGAATTTATGCGACAAAAAGGAGAATGAGAAAGAAGAAGAAAAGGAAAACCAAGTGAGTCGTGTTCCTTACAATTCAACATCACCTGGTTTAATATTAAAGGAATTGAAGCAGGCGAAATTGAATCTAAACCGGTCTACAAACGACATCGCTGATGTTCGAGTTTCCGTTGAATCGCTCAACAAGAAACTAGAGAAGGAAAGAATCTCTCTCGAGAAAACCCGCGCGAGGTTAACTCAGAATTCTTCGAAAATAACTTATCTAGAGGAAGAGCTAAACCAAACAAGACTTAAGCTGCAAGTAGTGAAAGAAGCTGAAATCGAATGTGGTTCTTCGGATGAACCTTCTGATGTTACGAAAGAGCTTCAACGGTTGAGTTCTGAGGCCGAACGGTTCAAGAAAATGGGAGAAGCTGCGAAATCGGAAGTCACGAGAGCAATGTCTGAGATTGAACAGACGAAAGCTATGATAAGAACATCCGAGATAAGGTTGGTGGCTGCTCGAAAAATGAAGGAAGCTGCTAAAGCGGCCGAAGCTGCTGCAATGGCCGAGATAAAAGCGTTATCTTATCACGATGGAGATTGTATGCAAAAGCGCGACGGAGTTACTCTAACCTTCGAAGAGTATAATGCTCTAACGTGCAAAGCTCGTGGCGCCGAGGAACAATCTAAGAAGAGAGTTCTTGACGCCATGTCTCAAGCTGAAGAAGCNAANTTTATCGAAAARGTGGTAACGGTTTTAAAAGGTAGGG
TARGET=127,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06099 Ps_contig_mira-and-tgicl-ass_franssen_41609
SEQUENCE=TATCAACGCAGAGTRNGGGGGGATTGGTGGCAGGAACCACAACTACAACACAAACACAGACACAGTACTTGTAACTAAGAAAACCTTCTCTTCAATTCATCACCACACTCCCAATCCCAACCACAACAACGATTCACTTCAACCTTCTATCATTTTCAAATCACACTACTGTCCATTCTTCAAGATTTGGACAGAGTGCATGTGAATCAGGAAACCATGACTTCTTCTACTCATGATCCCACAGATAACGAGGCTGATGGACAAGAGCAGTCAGAATCACAGATGCAGCCTGTATCTGCAAATGAAATTTCTCATGCTGGTATTGATACTCAGGTTGTTCAATACGTAGCACCTCCGCAGCTTGGCCCTGGACATGCTATGGTGCCACCTGCTTATCCATATCCGGATCCCTACTACAGAAGCATGTTTGCTCCCTATGATGCACAAGCTTATCCGCCACAGCCCTACGGTGGACATCCAATGGCCAATCTTCAGCTAATGGGAATTCAGCATGCAGGTGTTCCTTTACCAACTGATGCAGTCGAGGAGCCTGTGTTTGTCAATGCGAAACAGTATCATGGTATTTTAAGACGCAGGCAGTCTCGTGCTAAAGCTGAATCAGAAAAGAAAGTTACAAGGAATCGGAAGCCATACTTGCATGAATCACGACACTTGCACGCACTGAAAAGAGCAAGAGGATGCGGAGGTCGGTTCCTGAATTCAAAGAAAGACGAGAATCAACAGGACGAGGTTGGATCAGCTGACAACTCACATTCCAATATCAATCTCAATTCTGATAGAAATGATCTCGCACCATCTGATAAAAATTCTTGAAAAAAGTCAAAGAAATGGTGATGCTATGCATATCATGAGACAGATCACCAACCAACCAACAGCAACCTCAGATATATCTTTGTTGCTGATGTTTCATTGTGGCAGTGTACAATCCCATATGTATAGGTATAGGTTTCGTGTGGTAGCTAAATGTTAGGGGAGGTTGCAAGACAGAGCAAGTAAAATGTAAAATGAAGTGATTTAAGATCAAATGTTATCTCCTATGTGATCCATTCTAGCCTTTGTAGGTGAGATGATAGGCAAGATTATTATTGTATGCTTTCCAGCCTTTGTAGGTCAAATATAGGTGAGATTATTATTGTATGGCCATTTCTTTGTATTATTTGGTACTATGAAGAGATTAGAGATTATCAAGAGCCATTATGGTGTATTTGGATCATGTTTGTTTAGCTTGTGAAGAGACAGATTAGACC
TARGET=881,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06100 Ps_contig_mira-and-tgicl-ass_franssen_41615
SEQUENCE=CCCAACATTCCTTTTTTTTCTTCCTTGGGTCATATCTTTATTAACCTTTGTAAAAAGTTGTATCTGATTTGTTATTTGGCTTTGGACATAGCAGTGAGAATGTATAATAATGTCAGGCCGCAACTAGGGGTGCCTCAATTTTATTCTCAACCTCCAACAAGCTCACAACCTAATGTTTTAGGAAATGCATTTAATGCTGCCGGTTCTGGACTCATTCGAGGTGGGTTGGGTGCTTATGGAGAAAAAATATTCGGATCAAGCTCTGAGTACGTCCAAAGCAATATAAGTCGGTATTTCTCTGACCCCCAATATTACTTCCAAGTGAATGACCACTATGTTAAGAACAAATTAAAGGTGGTTTTATTCCCATTCCTGCACCGGGGTCATTGGACTAGAATTACCGAACCAGTGGGAGGTAGACTCTCTTATAAACCTCCAATTCATGACATAAATGCACCTGACCTGTACATTCCGTTGATGGCATTTGGTACCTATGTTGTTCTTGCTGGCATTTCACTCGGTCTTCGTGGAAAGTTTAGTCCGGAAGCATTAAACTGGTTATTCATCAAGGGATTGGTTGGTTGGTTTATGCAAACAGCGCTTCTTAAAGCGACATTACTTTCATTAGGAAGTGGAGAAGCGCCACTACTGGACATTATAGCATACGCAGGGTACACTTTTGCGGGCATATCTTTGGCTGTTGTTGGAAAAATAATCTCCGGTTACTCGTACTACGTTCTGATGCCATGGACCTGCTTATGCATGGGAGTATTCTTAGTTAAAACAATGAAAAGAGTCCTTTTTGCAGAAGTGAGGAGTTATGACTCAAGTAAACACCATTATCTCTTGCTGTTTATTGCTTTTGCTCAGTTCCCATTGTTCATATGGCTTGGAAACATTACTGTCAATTGGTTTATATGAAGTGATTGTATTTATACTGTACTATATAGTACCACTTAATGTTATTTTTTGGTTTGTGGATTTTGGATAATNTCTCTCTCTCTCTCTTCCTTTTATCCTATGTATACTTATTCCACAACTAAAAAATCATGTTCAGATTCAAATTATTAAATTTTATTCTTTTAAGTCGAAAT
TARGET=574,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1199
PRIMER_LEFT_PENALTY=0.113803
PRIMER_RIGHT_PENALTY=0.006089
PRIMER_LEFT_SEQUENCE=CTAGAATTACCGAACCAGTGGG
PRIMER_RIGHT_SEQUENCE=CCTGCGTATGCTATAATGTCCA
PRIMER_LEFT=391,22
PRIMER_RIGHT=670,22
PRIMER_LEFT_TM=59.886
PRIMER_RIGHT_TM=60.006
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=280
PRIMER_PAIR_PENALTY_1=0.2604
PRIMER_LEFT_1_PENALTY=0.256829
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=CATGACATAAATGCACCTGACC
PRIMER_RIGHT_1_SEQUENCE=TCCAACAACAGCCAAAGATATG
PRIMER_LEFT_1=441,22
PRIMER_RIGHT_1=707,22
PRIMER_LEFT_1_TM=60.257
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=5.8000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.2629
PRIMER_LEFT_2_PENALTY=0.256829
PRIMER_RIGHT_2_PENALTY=0.006089
PRIMER_LEFT_2_SEQUENCE=CATGACATAAATGCACCTGACC
PRIMER_RIGHT_2_SEQUENCE=CCTGCGTATGCTATAATGTCCA
PRIMER_LEFT_2=441,22
PRIMER_RIGHT_2=670,22
PRIMER_LEFT_2_TM=60.257
PRIMER_RIGHT_2_TM=60.006
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=230
PRIMER_PAIR_PENALTY_3=0.3134
PRIMER_LEFT_3_PENALTY=0.309780
PRIMER_RIGHT_3_PENALTY=0.003589
PRIMER_LEFT_3_SEQUENCE=CGGTCTTCGTGGAAAGTTTAGT
PRIMER_RIGHT_3_SEQUENCE=TCCAACAACAGCCAAAGATATG
PRIMER_LEFT_3=518,22
PRIMER_RIGHT_3=707,22
PRIMER_LEFT_3_TM=59.690
PRIMER_RIGHT_3_TM=59.996
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.7000
PRIMER_RIGHT_3_END_STABILITY=5.8000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=190
PRIMER_PAIR_PENALTY_4=0.3878
PRIMER_LEFT_4_PENALTY=0.309780
PRIMER_RIGHT_4_PENALTY=0.078029
PRIMER_LEFT_4_SEQUENCE=CGGTCTTCGTGGAAAGTTTAGT
PRIMER_RIGHT_4_SEQUENCE=CCTCACTTCTGCAAAAAGGACT
PRIMER_LEFT_4=518,22
PRIMER_RIGHT_4=815,22
PRIMER_LEFT_4_TM=59.690
PRIMER_RIGHT_4_TM=59.922
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_06101 Ps_contig_mira-and-tgicl-ass_franssen_41615
SEQUENCE=CCCAACATTCCTTTTTTTTCTTCCTTGGGTCATATCTTTATTAACCTTTGTAAAAAGTTGTATCTGATTTGTTATTTGGCTTTGGACATAGCAGTGAGAATGTATAATAATGTCAGGCCGCAACTAGGGGTGCCTCAATTTTATTCTCAACCTCCAACAAGCTCACAACCTAATGTTTTAGGAAATGCATTTAATGCTGCCGGTTCTGGACTCATTCGAGGTGGGTTGGGTGCTTATGGAGAAAAAATATTCGGATCAAGCTCTGAGTACGTCCAAAGCAATATAAGTCGGTATTTCTCTGACCCCCAATATTACTTCCAAGTGAATGACCACTATGTTAAGAACAAATTAAAGGTGGTTTTATTCCCATTCCTGCACCGGGGTCATTGGACTAGAATTACCGAACCAGTGGGAGGTAGACTCTCTTATAAACCTCCAATTCATGACATAAATGCACCTGACCTGTACATTCCGTTGATGGCATTTGGTACCTATGTTGTTCTTGCTGGCATTTCACTCGGTCTTCGTGGAAAGTTTAGTCCGGAAGCATTAAACTGGTTATTCATCAAGGGATTGGTTGGTTGGTTTATGCAAACAGCGCTTCTTAAAGCGACATTACTTTCATTAGGAAGTGGAGAAGCGCCACTACTGGACATTATAGCATACGCAGGGTACACTTTTGCGGGCATATCTTTGGCTGTTGTTGGAAAAATAATCTCCGGTTACTCGTACTACGTTCTGATGCCATGGACCTGCTTATGCATGGGAGTATTCTTAGTTAAAACAATGAAAAGAGTCCTTTTTGCAGAAGTGAGGAGTTATGACTCAAGTAAACACCATTATCTCTTGCTGTTTATTGCTTTTGCTCAGTTCCCATTGTTCATATGGCTTGGAAACATTACTGTCAATTGGTTTATATGAAGTGATTGTATTTATACTGTACTATATAGTACCACTTAATGTTATTTTTTGGTTTGTGGATTTTGGATAATNTCTCTCTCTCTCTCTTCCTTTTATCCTATGTATACTTATTCCACAACTAAAAAATCATGTTCAGATTCAAATTATTAAATTTTATTCTTTTAAGTCGAAAT
TARGET=994,14
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06102 Ps_contig_mira-and-tgicl-ass_franssen_41640
SEQUENCE=TACTCTTGGTTCCTATCCACCAACAAACCTACCGCCATCCACCACTTTGTTCATCGCCATCAACAAAACAACCGAAGAAGAAGAAAGAAATTTTCTGCAATAACTCACGTGAGGATCCGGTTTTCAACCTCTTCCAATCTCGGTTTCACCATCTGCTTCTTCCATGGCACCACCATCACAATCCAATTTGCAGCGACTCTTCAACCCGGTGAGTTTTCTTTAAGTCGCGCTTAAGATCCCGTTGATTTAGCAGCGCTGTGAGCACGGTGGTGAATGGTAAATTCGACTTCGAAGCATNAATTTCTGAGGCGCGAAGGTAATTTATTGATGTTGCACACGACCTAAGACTCGCGAGGCAATCGAACGTGATCGAACCGTTGGAAGCCCAATCCCGGAAGAATAACAGAAAGCTTCGAAACGGAATCCATGCCTGATTTTGGAAGTGAGAGCGAAGCAGTTGTTTTGAGCAATTTCGTTGAAAAGCGGCGGCGAGCGAGAAGCCGGATTCTTGCGTTTGCATAAGCCGTTTTCCCCAATTTGATTCAACTTTTCTTTTACGTGATTTTCCGTTTTGATAAGTTAGGACTCGACTACTTTTTATGCTTCAGTTGARGTATTGTGGTGAGAGTATTAGGAAAGGAAAGAGACGTAAGGGTAGGTTCGTTAGTTTTTAGG
TARGET=74,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06103 Ps_contig_mira-and-tgicl-ass_franssen_41647
SEQUENCE=GAAGAAGGTGTTAAGGCCAAGAGTAAAAATGGTGGGTTTAAACTTCTAGGATTAAGATTAGGAATCCTTCTATTAGAAGGCTGTGTAGTGGAGCAATTGCCGGGGCGATTTCTCGTACCACTGTGGCGCCATTGGAGACTATTAGGACTCATTTGATGGTTGGGAGTAGTGGTCATTCTAGCACTGAGGTTTTCAATGATATTATGAAAACGGATGGATGGAAGGGTTTGTTTAGGGGTAATCTAGTTAATGTCATTCGTGTTGCGCCGAGCAAGGCCATTGAGCTATTTGCTTATGATACGGTAAACAAGAACCTATCCGCAAAGCCCGGGGAGCAATCCAAACTCCCAGTTCCTGCATCATTGATTGCAGGTGCCTGTGCTGGAGTGAGTTCAACTATATGCACATATCCACTTGAACTACTCAAGACTAGACTAACTATCCAGAGGGGTGTTTATAATGGTCTCCTTGATGCATTCGTGAAAATAATTAAAGAAGAGGGTCCTGCCGAACTATACAGAGGCCTTACTCCTAGTCTTATTGGTGTTATTCCATATTCTGCTACCAATTACTTTGCCTATGACACACTACGCAAAGCATACCAGAAAATTTTCAAACAAGAGAAGATTGGCAACATTGAAACCCTTTTGATTGGTTCAGCAGCTGGTGCTATTTCAAGTACTGCGACTTTTCCACTCGAGGTGGCTCGCAAGCAAATGCAAGTTGGAGCTCTCAGTGGAAGACAGGTTTACAGAGATGTCCTTCATGCACTTGTTAGCATACTAGAAAAAGAAGGGATCCAGGGTCTATATAGAGGGCTTGGACCTAGTTGCATGAAGTTAGTGCCGGCCGCAGGAATCTCTTTCATGTGTTACGAAGCATGCAAGAAGATACTTATAGATAATGACGAGAAGGAATGAATGAATTAAGTGTTTTTGTTTCCACTGAAATTAAGTTGTGGAACTGCTGCATACCTTTTTTAAGCATTTGGTTAACTTACTTTGGGTTTTCTTGTATTGTTGGATTTTTCCGTCACCCCCGCAAAAGTCTTGTGTCTGAGAATGGAATGATGTATTTTTTTTGTTTATTTATGGTTGCAGCCAGGTTTTTCATTGTGTCTTGGCTGCATGATTTCAAGGTCAAAATCTTT
TARGET=915,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0922
PRIMER_LEFT_PENALTY=0.052366
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=GTGTTACGAAGCATGCAAGAAG
PRIMER_RIGHT_SEQUENCE=AGACACAATGAAAAACCTGGCT
PRIMER_LEFT=868,22
PRIMER_RIGHT=1119,22
PRIMER_LEFT_TM=59.948
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=252
PRIMER_PAIR_PENALTY_1=0.2397
PRIMER_LEFT_1_PENALTY=0.052366
PRIMER_RIGHT_1_PENALTY=0.187326
PRIMER_LEFT_1_SEQUENCE=GTGTTACGAAGCATGCAAGAAG
PRIMER_RIGHT_1_SEQUENCE=TGCAGCAGTTCCACAACTTAAT
PRIMER_LEFT_1=868,22
PRIMER_RIGHT_1=970,22
PRIMER_LEFT_1_TM=59.948
PRIMER_RIGHT_1_TM=59.813
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=103
PRIMER_PAIR_PENALTY_2=0.2401
PRIMER_LEFT_2_PENALTY=0.052729
PRIMER_RIGHT_2_PENALTY=0.187326
PRIMER_LEFT_2_SEQUENCE=AAGTTGGAGCTCTCAGTGGAAG
PRIMER_RIGHT_2_SEQUENCE=TGCAGCAGTTCCACAACTTAAT
PRIMER_LEFT_2=720,22
PRIMER_RIGHT_2=970,22
PRIMER_LEFT_2_TM=60.053
PRIMER_RIGHT_2_TM=59.813
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=251
PRIMER_PAIR_PENALTY_3=0.2460
PRIMER_LEFT_3_PENALTY=0.058624
PRIMER_RIGHT_3_PENALTY=0.187326
PRIMER_LEFT_3_SEQUENCE=GAGCTCTCAGTGGAAGACAGGT
PRIMER_RIGHT_3_SEQUENCE=TGCAGCAGTTCCACAACTTAAT
PRIMER_LEFT_3=726,22
PRIMER_RIGHT_3=970,22
PRIMER_LEFT_3_TM=60.059
PRIMER_RIGHT_3_TM=59.813
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.2647
PRIMER_LEFT_4_PENALTY=0.077340
PRIMER_RIGHT_4_PENALTY=0.187326
PRIMER_LEFT_4_SEQUENCE=GCTCTCAGTGGAAGACAGGTTT
PRIMER_RIGHT_4_SEQUENCE=TGCAGCAGTTCCACAACTTAAT
PRIMER_LEFT_4=728,22
PRIMER_RIGHT_4=970,22
PRIMER_LEFT_4_TM=59.923
PRIMER_RIGHT_4_TM=59.813
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=243
=
PRIMER_SEQUENCE_ID=SSR_06104 Ps_contig_mira-and-tgicl-ass_franssen_41650
SEQUENCE=AAAATCCCCTCATTCTAAACTTCAAAATCAATTTTACACAATTCCAAAAACAAAAAAGTAGTTGCTAGTAAGTACAGTAAATGGCTTCAACAGCAACTAATTATGAATATTAAATCCTCTACTTCACAATCAGGCATTTACATGTTTAGTGTACAAGGTAGACAGACAGACAATACATGCATATTGTCCAAATAGCCCTCTAAATGAATACATAAATGAGTCACTTGCAGCACTGCCCTCTAAATGATGCAGCTTCTATAGGATGAACTAAAGATTGTCAGGGTTCTGCAGTATACCCATCTATAGTTTTTTTAATTGCATCCAACTGAGACATGACATATTCCACCTTCTTGAAACATATCTCATTGGCTATTCTTGGACCATAATCACATTTTTCAAATGGACGGTATTCACAAGCACTTTTGATTTCATCACGCAACGCTACTTGGATGTCCTTGATTAATTGCATGAACTCGRAGGCAGTGTATTTNGAACAAGTATYYTTTCNGGACCATGGACTTGACAAGCTCATCCATCGACCCTTCC
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06105 Ps_contig_mira-and-tgicl-ass_franssen_41653
SEQUENCE=TTTCCTTGTCTTTTACTTCTATCAACAAAAAGTTAAGTTAGTCATCTACTAGTAGTATTAGTCCTTATTAGCTCTTTAATTTCATCAAATGGATTTTCTTCCCATAATCCATTTCTTTCTCTATCCACTGACTAACACTTCCACCATTATAGAGATAATCAACATCACTAATATTCTGCTCTACAAAAGCTACATCATCATCATCATCATCATCATCATCATTATCATACTTTTCAACAAGCTCTTGATCTATGACATGGTTGATCTGGCCTTTGCTATTGCTTTGAGAGGGACCAGTGGAACTTTCTTGTACTGAAGTAAAGGAATTTCCACTACAAAAGACTTGAAGATGCTGCTGCTGATGAGTAAGGAAAAGGACCTGTGAATGATAATGATGGAGGGAGATGATTATTGTTGTAATTTTGAAGAAGTAGGAAGTTAAAAAGGGTAACTTGTTGAGTATTTCATCCTCCACTAGTA
TARGET=193,30
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0838
PRIMER_LEFT_PENALTY=0.065960
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=CCACTGACTAACACTTCCACCA
PRIMER_RIGHT_SEQUENCE=TTTTCCTTACTCATCAGCAGCA
PRIMER_LEFT=124,22
PRIMER_RIGHT=374,22
PRIMER_LEFT_TM=60.066
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.0895
PRIMER_LEFT_1_PENALTY=0.065960
PRIMER_RIGHT_1_PENALTY=0.023556
PRIMER_LEFT_1_SEQUENCE=CCACTGACTAACACTTCCACCA
PRIMER_RIGHT_1_SEQUENCE=TCATTCACAGGTCCTTTTCCTT
PRIMER_LEFT_1=124,22
PRIMER_RIGHT_1=388,22
PRIMER_LEFT_1_TM=60.066
PRIMER_RIGHT_1_TM=59.976
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.1039
PRIMER_LEFT_2_PENALTY=0.065960
PRIMER_RIGHT_2_PENALTY=0.037936
PRIMER_LEFT_2_SEQUENCE=CCACTGACTAACACTTCCACCA
PRIMER_RIGHT_2_SEQUENCE=GCCAGATCAACCATGTCATAGA
PRIMER_LEFT_2=124,22
PRIMER_RIGHT_2=269,22
PRIMER_LEFT_2_TM=60.066
PRIMER_RIGHT_2_TM=59.962
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.1963
PRIMER_LEFT_3_PENALTY=0.065960
PRIMER_RIGHT_3_PENALTY=0.130358
PRIMER_LEFT_3_SEQUENCE=CCACTGACTAACACTTCCACCA
PRIMER_RIGHT_3_SEQUENCE=ACTGGTCCCTCTCAAAGCAATA
PRIMER_LEFT_3=124,22
PRIMER_RIGHT_3=297,22
PRIMER_LEFT_3_TM=60.066
PRIMER_RIGHT_3_TM=60.130
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=0.2493
PRIMER_LEFT_4_PENALTY=0.065960
PRIMER_RIGHT_4_PENALTY=0.183296
PRIMER_LEFT_4_SEQUENCE=CCACTGACTAACACTTCCACCA
PRIMER_RIGHT_4_SEQUENCE=CTTACTCATCAGCAGCAGCATC
PRIMER_LEFT_4=124,22
PRIMER_RIGHT_4=369,22
PRIMER_LEFT_4_TM=60.066
PRIMER_RIGHT_4_TM=60.183
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_06106 Ps_contig_mira-and-tgicl-ass_franssen_41655
SEQUENCE=TGCAGCAATCAACGAACTAAATACGTCCAAACTAACCCCTCCAATTTTCTAAACCAACCGTTTGTNTAAAACGAAATCAGGTATACTTCCATATCATCACCACCACCACCATTGTTATTTCCACCACCACCACAAGATGCATCAACAACACCACCATCTTGAGATTTAGGGTGAAAAACAGAGGAAGCCCCTAGAATTACACAATTATCAAGAGTCGATCCAAAATCAGCTCTCCACTTCAACAAAACCCCATCCTCTATCCCTAATTCCCCACTGGGAAGCTCGATCCGAAGCAACCGAATCTCATTAAAATTCTTCAAAACCTGAGTAGGCGAGTGATGTGTAACACCGCCATCTCCATCATCCTCACTTCCAACAGCAAGCGGCGAAGACGACGAAGAAGACGAATTCCCGGAATTGGAACCGGAACCTCTCTTCGGACCCAGAAACTGACCAAGTGTTTGAATCGGCTTAGCAATCCCACCAAAAACCAACCTCAGAAGATTCCAAAACGGGCCACGAGATTTATCAGAAGAAGCCGACGAAGAATTGGAATCATCATCAGAAATAACACAATCAACGCGAACAACAACGTTTTCAACCTGAGGAACTAAAGAGTGAAAACGGCGCGAAACGACGCAGCATCTCCCCAAGGCTTTAACATCACCGATCTTGTTGAAAACTAAGAGAAGAAGAGAATCAGGGATTCGATCGAAATGATCGATTTGGGGGAAATAGGGTTCGGGGTAGATCCGATTGGGATTGGGATTGAGATTGGAAGGTTGATCTGAACGAAGTGAAGACATGGTGTTAAAGATCGGAAAGAGAGTTGATTTGGGGGTAGATCTGGTTTTAGGGTTGAAGCATGATCGGAGAAGTGGTGAAGG
TARGET=98,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1305
PRIMER_LEFT_PENALTY=0.127450
PRIMER_RIGHT_PENALTY=0.003045
PRIMER_LEFT_SEQUENCE=CTCCAATTTTCTAAACCAACCG
PRIMER_RIGHT_SEQUENCE=GGGGAATTAGGGATAGAGGATG
PRIMER_LEFT=38,22
PRIMER_RIGHT=271,22
PRIMER_LEFT_TM=59.873
PRIMER_RIGHT_TM=60.003
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.1305
PRIMER_LEFT_1_PENALTY=0.127450
PRIMER_RIGHT_1_PENALTY=0.003045
PRIMER_LEFT_1_SEQUENCE=CTCCAATTTTCTAAACCAACCG
PRIMER_RIGHT_1_SEQUENCE=GAATTAGGGATAGAGGATGGGG
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=268,22
PRIMER_LEFT_1_TM=59.873
PRIMER_RIGHT_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.1305
PRIMER_LEFT_2_PENALTY=0.127450
PRIMER_RIGHT_2_PENALTY=0.003045
PRIMER_LEFT_2_SEQUENCE=CTCCAATTTTCTAAACCAACCG
PRIMER_RIGHT_2_SEQUENCE=GGAATTAGGGATAGAGGATGGG
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=269,22
PRIMER_LEFT_2_TM=59.873
PRIMER_RIGHT_2_TM=60.003
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.1305
PRIMER_LEFT_3_PENALTY=0.127450
PRIMER_RIGHT_3_PENALTY=0.003045
PRIMER_LEFT_3_SEQUENCE=CTCCAATTTTCTAAACCAACCG
PRIMER_RIGHT_3_SEQUENCE=GGGAATTAGGGATAGAGGATGG
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=270,22
PRIMER_LEFT_3_TM=59.873
PRIMER_RIGHT_3_TM=60.003
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.1305
PRIMER_LEFT_4_PENALTY=0.127450
PRIMER_RIGHT_4_PENALTY=0.003045
PRIMER_LEFT_4_SEQUENCE=CTCCAATTTTCTAAACCAACCG
PRIMER_RIGHT_4_SEQUENCE=TGGGGAATTAGGGATAGAGGAT
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=272,22
PRIMER_LEFT_4_TM=59.873
PRIMER_RIGHT_4_TM=60.003
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_06107 Ps_contig_mira-and-tgicl-ass_franssen_41655
SEQUENCE=TGCAGCAATCAACGAACTAAATACGTCCAAACTAACCCCTCCAATTTTCTAAACCAACCGTTTGTNTAAAACGAAATCAGGTATACTTCCATATCATCACCACCACCACCATTGTTATTTCCACCACCACCACAAGATGCATCAACAACACCACCATCTTGAGATTTAGGGTGAAAAACAGAGGAAGCCCCTAGAATTACACAATTATCAAGAGTCGATCCAAAATCAGCTCTCCACTTCAACAAAACCCCATCCTCTATCCCTAATTCCCCACTGGGAAGCTCGATCCGAAGCAACCGAATCTCATTAAAATTCTTCAAAACCTGAGTAGGCGAGTGATGTGTAACACCGCCATCTCCATCATCCTCACTTCCAACAGCAAGCGGCGAAGACGACGAAGAAGACGAATTCCCGGAATTGGAACCGGAACCTCTCTTCGGACCCAGAAACTGACCAAGTGTTTGAATCGGCTTAGCAATCCCACCAAAAACCAACCTCAGAAGATTCCAAAACGGGCCACGAGATTTATCAGAAGAAGCCGACGAAGAATTGGAATCATCATCAGAAATAACACAATCAACGCGAACAACAACGTTTTCAACCTGAGGAACTAAAGAGTGAAAACGGCGCGAAACGACGCAGCATCTCCCCAAGGCTTTAACATCACCGATCTTGTTGAAAACTAAGAGAAGAAGAGAATCAGGGATTCGATCGAAATGATCGATTTGGGGGAAATAGGGTTCGGGGTAGATCCGATTGGGATTGGGATTGAGATTGGAAGGTTGATCTGAACGAAGTGAAGACATGGTGTTAAAGATCGGAAAGAGAGTTGATTTGGGGGTAGATCTGGTTTTAGGGTTGAAGCATGATCGGAGAAGTGGTGAAGG
TARGET=121,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1305
PRIMER_LEFT_PENALTY=0.127450
PRIMER_RIGHT_PENALTY=0.003045
PRIMER_LEFT_SEQUENCE=CTCCAATTTTCTAAACCAACCG
PRIMER_RIGHT_SEQUENCE=GGGGAATTAGGGATAGAGGATG
PRIMER_LEFT=38,22
PRIMER_RIGHT=271,22
PRIMER_LEFT_TM=59.873
PRIMER_RIGHT_TM=60.003
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=234
PRIMER_PAIR_PENALTY_1=0.1305
PRIMER_LEFT_1_PENALTY=0.127450
PRIMER_RIGHT_1_PENALTY=0.003045
PRIMER_LEFT_1_SEQUENCE=CTCCAATTTTCTAAACCAACCG
PRIMER_RIGHT_1_SEQUENCE=GAATTAGGGATAGAGGATGGGG
PRIMER_LEFT_1=38,22
PRIMER_RIGHT_1=268,22
PRIMER_LEFT_1_TM=59.873
PRIMER_RIGHT_1_TM=60.003
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=11.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.1305
PRIMER_LEFT_2_PENALTY=0.127450
PRIMER_RIGHT_2_PENALTY=0.003045
PRIMER_LEFT_2_SEQUENCE=CTCCAATTTTCTAAACCAACCG
PRIMER_RIGHT_2_SEQUENCE=GGAATTAGGGATAGAGGATGGG
PRIMER_LEFT_2=38,22
PRIMER_RIGHT_2=269,22
PRIMER_LEFT_2_TM=59.873
PRIMER_RIGHT_2_TM=60.003
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.9000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=232
PRIMER_PAIR_PENALTY_3=0.1305
PRIMER_LEFT_3_PENALTY=0.127450
PRIMER_RIGHT_3_PENALTY=0.003045
PRIMER_LEFT_3_SEQUENCE=CTCCAATTTTCTAAACCAACCG
PRIMER_RIGHT_3_SEQUENCE=GGGAATTAGGGATAGAGGATGG
PRIMER_LEFT_3=38,22
PRIMER_RIGHT_3=270,22
PRIMER_LEFT_3_TM=59.873
PRIMER_RIGHT_3_TM=60.003
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.9000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.1305
PRIMER_LEFT_4_PENALTY=0.127450
PRIMER_RIGHT_4_PENALTY=0.003045
PRIMER_LEFT_4_SEQUENCE=CTCCAATTTTCTAAACCAACCG
PRIMER_RIGHT_4_SEQUENCE=TGGGGAATTAGGGATAGAGGAT
PRIMER_LEFT_4=38,22
PRIMER_RIGHT_4=272,22
PRIMER_LEFT_4_TM=59.873
PRIMER_RIGHT_4_TM=60.003
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_06108 Ps_contig_mira-and-tgicl-ass_franssen_41675
SEQUENCE=CGGTATATCAATAAACCACCGCCGGGGAGAAACTCCGACGCCTCCACTCGCGGCGGATCTTCATCGCCGAGGGAGAATCAGTATCTCTTTATGCAAGAAGGCGAAATGGCTTCGTGGCTTCATTATCCTAATCCCGATGATGATGATTCTCCTTTGGATCAATCCTTCTGTGCTGATTTCCTTAACCAGCCGTCGGCTGTTAATAACAATAGTACCATGCAAACTCCACCACGGAGGTCCGAGCAGGTGCCTCCGGTTAGAGCCGAACCAGAATCATTGTCGTATTTTGAGAGTCTTTTGAATCAACGGTTGTAGATTCGTGCGATACTCCAG
TARGET=136,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2667
PRIMER_LEFT_PENALTY=0.171245
PRIMER_RIGHT_PENALTY=0.095445
PRIMER_LEFT_SEQUENCE=CGTGGCTTCATTATCCTAATCC
PRIMER_RIGHT_SEQUENCE=AATGATTCTGGTTCGGCTCTAA
PRIMER_LEFT=112,22
PRIMER_RIGHT=277,22
PRIMER_LEFT_TM=59.829
PRIMER_RIGHT_TM=60.095
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=0.5073
PRIMER_LEFT_1_PENALTY=0.447441
PRIMER_RIGHT_1_PENALTY=0.059875
PRIMER_LEFT_1_SEQUENCE=GTATATCAATAAACCACCGCCG
PRIMER_RIGHT_1_SEQUENCE=ACAGAAGGATTGATCCAAAGGA
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=170,22
PRIMER_LEFT_1_TM=60.447
PRIMER_RIGHT_1_TM=59.940
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=13.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=169
PRIMER_PAIR_PENALTY_2=0.5073
PRIMER_LEFT_2_PENALTY=0.447441
PRIMER_RIGHT_2_PENALTY=0.059875
PRIMER_LEFT_2_SEQUENCE=CGGTATATCAATAAACCACCGC
PRIMER_RIGHT_2_SEQUENCE=ACAGAAGGATTGATCCAAAGGA
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=170,22
PRIMER_LEFT_2_TM=60.447
PRIMER_RIGHT_2_TM=59.940
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=11.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=171
PRIMER_PAIR_PENALTY_3=0.5150
PRIMER_LEFT_3_PENALTY=0.447441
PRIMER_RIGHT_3_PENALTY=0.067516
PRIMER_LEFT_3_SEQUENCE=GTATATCAATAAACCACCGCCG
PRIMER_RIGHT_3_SEQUENCE=CAGAAGGATTGATCCAAAGGAG
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=169,22
PRIMER_LEFT_3_TM=60.447
PRIMER_RIGHT_3_TM=60.068
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=13.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=168
PRIMER_PAIR_PENALTY_4=0.5150
PRIMER_LEFT_4_PENALTY=0.447441
PRIMER_RIGHT_4_PENALTY=0.067516
PRIMER_LEFT_4_SEQUENCE=CGGTATATCAATAAACCACCGC
PRIMER_RIGHT_4_SEQUENCE=CAGAAGGATTGATCCAAAGGAG
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=169,22
PRIMER_LEFT_4_TM=60.447
PRIMER_RIGHT_4_TM=60.068
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=11.1000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=170
=
PRIMER_SEQUENCE_ID=SSR_06109 Ps_contig_mira-and-tgicl-ass_franssen_41680
SEQUENCE=GGGAATTCACAAAATCACAATACAAATATTTCAATCATAACCCCTACCTCTCATTTTCTCTATCTAAACTCCCAACAAATCTCTCTCTCTTTCTCTCTCTAGACCGTTACAACAATGTCCTCCTCCGCACCACCCGTTCTCCCCGTCGCCAACGTTCCTCCTCCTCCACCAATCGGATCCACCGTCGCTGCCACCGGATCTGATGCTCCTGCCAACTCCCCAGCCGTCCGTGTTCTCCTTAACAACCTCTCCGAGTCTCTCCGCCACGGCCTCGCCTTGCGCCGCCCTTGGACGGAACTCGTTGACAGATCCGCCTTCTCGAAGCCTGAATCCTTCTCCGAAGCGACTCTCCGTGTCCGCAAGAATTACTCATACTTCCGTGTCAATTACTACGCCATCGTCGCCGGGATCCTCGCCGTGTCTCTCCTGACGAATCCGTTCTCTCTCATTCTCCTCGTCGGCCTCCTCGCTTCGTGGACGTTTCTCTACCTCTTCCGTCCCTCCGATCGTCCTCTCGTTATCCTCGGTCGCACTTACACTGATTTCGAAACCCTAGCGATTCTCTCAGGACTCACTCTTATCGTTGTGTTTCTCACCAGCGTTGGTTCTGTTCTCGTCTCAGCTTTAATGCTTGGCGTTGCTGTTGTTTGTATCCATGGAGCATTCCGCGCACCTGAGGATCTTTTCCTTGATGATCAGGATAACTCTCAGGCTACCGGATTCCTCTCGTTCCTCCGCACTCCTGTTGCAGCCGCTCGTTYCYTCYNTCACCGGTTACCCGGGACGGTCGTTTGAGTGTTGGTTGAAGATATTCTTA
TARGET=80,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06110 Ps_contig_mira-and-tgicl-ass_franssen_41680
SEQUENCE=GGGAATTCACAAAATCACAATACAAATATTTCAATCATAACCCCTACCTCTCATTTTCTCTATCTAAACTCCCAACAAATCTCTCTCTCTTTCTCTCTCTAGACCGTTACAACAATGTCCTCCTCCGCACCACCCGTTCTCCCCGTCGCCAACGTTCCTCCTCCTCCACCAATCGGATCCACCGTCGCTGCCACCGGATCTGATGCTCCTGCCAACTCCCCAGCCGTCCGTGTTCTCCTTAACAACCTCTCCGAGTCTCTCCGCCACGGCCTCGCCTTGCGCCGCCCTTGGACGGAACTCGTTGACAGATCCGCCTTCTCGAAGCCTGAATCCTTCTCCGAAGCGACTCTCCGTGTCCGCAAGAATTACTCATACTTCCGTGTCAATTACTACGCCATCGTCGCCGGGATCCTCGCCGTGTCTCTCCTGACGAATCCGTTCTCTCTCATTCTCCTCGTCGGCCTCCTCGCTTCGTGGACGTTTCTCTACCTCTTCCGTCCCTCCGATCGTCCTCTCGTTATCCTCGGTCGCACTTACACTGATTTCGAAACCCTAGCGATTCTCTCAGGACTCACTCTTATCGTTGTGTTTCTCACCAGCGTTGGTTCTGTTCTCGTCTCAGCTTTAATGCTTGGCGTTGCTGTTGTTTGTATCCATGGAGCATTCCGCGCACCTGAGGATCTTTTCCTTGATGATCAGGATAACTCTCAGGCTACCGGATTCCTCTCGTTCCTCCGCACTCCTGTTGCAGCCGCTCGTTYCYTCYNTCACCGGTTACCCGGGACGGTCGTTTGAGTGTTGGTTGAAGATATTCTTA
TARGET=156,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06111 Ps_contig_mira-and-tgicl-ass_franssen_41701
SEQUENCE=TTATTATTATTATTACAATCAAGAACAAAATCATGATTGGTAGATACATTTAAATCAAATAATGAAACATTCTACCTTTGAGATTGTGATATCTTCTTCTTCATGATCAATAGATTGTTGTTTCTTCTTTTCATGTTGATTTTGTAATGGTGTGTTTGGACTTGGAGGAAATTCTTGACAAGAGAAGATGTCATGGTTCCATTGAAGATAATAATTCCTTAACCCTTTGTTGGTATGTTTAAGGAATTTTTGAGTTGTTGATAGTTTGACAAACACCTTAAATGGATAAAAAGTAGGAGAAGATTTTTAGAGGCAGACAAAAGTTGTGTTCCAATTATGATTCTACACATGGATATGG
TARGET=1,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06112 Ps_contig_mira-and-tgicl-ass_franssen_41717
SEQUENCE=CCAAAACCTACTTTCCCCCCTTCCGTCTAGAAAGGGGAAGGAAGGTAGTCACACTCCTCTNAAATCAYNGNNNYCCTACGCTAGCGATGAATGTTAAGCAAGTCTTTAGCTTCAGTCTGCTCCTCCATGAGAACATCACTAACATACTTATTCAACAAATCCGTCTTCTTCTTCTGCAACACCATCTTCTCAATCTCCTTTTCATCAGGCAACGGCACGTGAACAATGAATTCCCTCTCTTTCATATCCGACTTATCCCTCATCTCCCTCTCCTTCATCCTCTCCTCCTCCACCACATCTTCCTCCTCCTCATGAAGTATCTCCCTAGCAGCAGTTGTAACCTCAGCAACCTCCCCACTTCGAACAGCCTTCCTAGCCTCTTTCCTTATCATATCAAGCCGGTGCCACTCTTCGTCCGCTTCACGTTGCATATCCTTCTCAGCAGCCGCCTCAAGGGGTTCAAGAATACCATCCTCATCGTCCCGGTAACCATAATAAGCTGCATTGATTCGTTTATAGATGTCATATCGAGTTCTTCGTTTGCGAAGCTCCGGAGGTTTCTCAAACAGCTCACGTACTCCGGGGAGCTTCTTCGCAGCACCAAAGTATCGGTAGCCAGGACCACGGCCGCTAGGGTTTGGTACATCTACAATATTTCCGTCGAGATCCGTCATCTTGGCAGAATGTCGAGCGTAATTGGGACCGCCGAGTTCGACAATTCGCCGCTCCCAATGAACCTTCTCGCGGATGAGTTTATTGATTTCGTCATTGAGGTCACGGAGTCGGTGTTCGCCGAGACCTTCGTTTTGGATCTCGGCGACTTTACGACCGATTTCACGCATAATCTGTTGACGCCATTTGTCTGCTTCGTTGAGGTCACGGCACTCTGAGGCAAGAAAAGGACGGCGTTCCTTTGGTTTCTTCTTCTCTTCCGCTTTCATGGTGATGAAACGGTTGAGCATGGATTGAGCTTTCTCTTCATTACGAGCCATTTTCGATTTCTTCTGAATGGAAAATTGGAAAACACTGCAAATTCAGCGTAGAGGTTTTGAAATAGGGTTTCCTCTTGTTCTCAGATNGGAGGAAGGAGTTTAGCGTTTCTTAACCCCCCGAC
TARGET=164,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06113 Ps_contig_mira-and-tgicl-ass_franssen_41732
SEQUENCE=AGACACCGGAACATGATGATGATGAGTTCTTTTTTTCATTGCCTTGTAGTAATGGTGTGGTAATGATGAAGACTCAAATTTGATGAGATTGGTGAAAAAACTGAAAAGTTTTGGTTGGTTTAAGGTGAATGATGTTGAATTCTGGTGGAGGAGAAGATGAACTGTATGAACAATTATGGAAGGCTTGTGCAGGTCCTCATGTTGAAGTTCCTCGTGCTGGACAAAGGGTCTTCTATTTTCCTCAAGGACACATGGAACAATTAGAAGTATCAACAAATCAGGAACTAAATCAGAGGATTCCATTGTTCAAACTTCCCAACAAGATCCTTTGTCGTGTTGTCAATGTTCATTTACTGGCTGAACAAGAAACAGATGAGGTTTATGCACAGATAACTTTGGTACCAGAATCTAAACAAACCGAGCCTACGAGCCTTGATCCTTGTCCTGTTGAGCCTCCGAAACCGAGAACTCACTTATTCAGCAAGGTCTTGACTAACTCTGATACCAGTACTCATGGTGGCTTTTCTGTTCCTCGGAAGCCCGCCAACGAATGTCTTCCCTTGTTGGATCTGTCACAATCTACTCCAACTCAGGAATTGGTTGCTAGAGATCTTCACAACTATGAATGGCACTTTAAGCATATCTTTAGAGGTCAACCACGTCGACACTTGCTCACAACTGGATGGAGTACTTTTCTGACTTCCAAGAGATTAGTCGCCGGAG
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06114 Ps_contig_mira-and-tgicl-ass_franssen_41735
SEQUENCE=CGGCGCCATGAACAACAACAATAGAGCGAGATATCCACCTCCCGGGATCGCTCCTGGACGCGGAGGAGCGAATCCCAACGTGAATCTGAACCAGAACCCGAACCTGAACCATCAGTTTCAGCAAAGACCACCTTACCACCACCATAACCAACAGCAACATCACCAGCCGCAACAGCACCAGCCACAACATCAGCAGCAGCAGTACTATGCGCAGAGACAAATGGTGCAGCAACACCAGCAGCAACATCAGCAGCAGCAGCAGCAACAACAGTGGCTGAGAAGGGCACAGTTGGGTGGTTCGGAATCCAACGCTGCCGAGGAGGTCGAGAAGTCCGTGCAGTCTGAAGCCAATGACTCAAGTTCTCAAGACTGGAAGGCCGGACTAAAGATTCCACCAG
TARGET=191,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1049
PRIMER_LEFT_PENALTY=0.093026
PRIMER_RIGHT_PENALTY=1.011889
PRIMER_LEFT_SEQUENCE=ACCACCTTACCACCACCATAAC
PRIMER_RIGHT_SEQUENCE=GAGTCATTGGCTTCAGACTGC
PRIMER_LEFT=126,22
PRIMER_RIGHT=356,21
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=1.1100
PRIMER_LEFT_1_PENALTY=0.093026
PRIMER_RIGHT_1_PENALTY=1.016986
PRIMER_LEFT_1_SEQUENCE=ACCACCTTACCACCACCATAAC
PRIMER_RIGHT_1_SEQUENCE=AACTTGAGTCATTGGCTTCAGA
PRIMER_LEFT_1=126,22
PRIMER_RIGHT_1=361,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=58.983
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=1.1100
PRIMER_LEFT_2_PENALTY=0.093026
PRIMER_RIGHT_2_PENALTY=1.016986
PRIMER_LEFT_2_SEQUENCE=ACCACCTTACCACCACCATAAC
PRIMER_RIGHT_2_SEQUENCE=TGAGAACTTGAGTCATTGGCTT
PRIMER_LEFT_2=126,22
PRIMER_RIGHT_2=365,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=58.983
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=1.1100
PRIMER_LEFT_3_PENALTY=0.093026
PRIMER_RIGHT_3_PENALTY=1.016986
PRIMER_LEFT_3_SEQUENCE=ACCACCTTACCACCACCATAAC
PRIMER_RIGHT_3_SEQUENCE=CTTGAGAACTTGAGTCATTGGC
PRIMER_LEFT_3=126,22
PRIMER_RIGHT_3=367,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=58.983
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=1.2228
PRIMER_LEFT_4_PENALTY=0.093026
PRIMER_RIGHT_4_PENALTY=1.129727
PRIMER_LEFT_4_SEQUENCE=ACCACCTTACCACCACCATAAC
PRIMER_RIGHT_4_SEQUENCE=GGCCTTCCAGTCTTGAGAACTT
PRIMER_LEFT_4=126,22
PRIMER_RIGHT_4=378,22
PRIMER_LEFT_4_TM=59.907
PRIMER_RIGHT_4_TM=61.130
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_06115 Ps_contig_mira-and-tgicl-ass_franssen_41735
SEQUENCE=CGGCGCCATGAACAACAACAATAGAGCGAGATATCCACCTCCCGGGATCGCTCCTGGACGCGGAGGAGCGAATCCCAACGTGAATCTGAACCAGAACCCGAACCTGAACCATCAGTTTCAGCAAAGACCACCTTACCACCACCATAACCAACAGCAACATCACCAGCCGCAACAGCACCAGCCACAACATCAGCAGCAGCAGTACTATGCGCAGAGACAAATGGTGCAGCAACACCAGCAGCAACATCAGCAGCAGCAGCAGCAACAACAGTGGCTGAGAAGGGCACAGTTGGGTGGTTCGGAATCCAACGCTGCCGAGGAGGTCGAGAAGTCCGTGCAGTCTGAAGCCAATGACTCAAGTTCTCAAGACTGGAAGGCCGGACTAAAGATTCCACCAG
TARGET=248,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1049
PRIMER_LEFT_PENALTY=0.093026
PRIMER_RIGHT_PENALTY=1.011889
PRIMER_LEFT_SEQUENCE=ACCACCTTACCACCACCATAAC
PRIMER_RIGHT_SEQUENCE=GAGTCATTGGCTTCAGACTGC
PRIMER_LEFT=126,22
PRIMER_RIGHT=356,21
PRIMER_LEFT_TM=59.907
PRIMER_RIGHT_TM=60.012
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=1.1100
PRIMER_LEFT_1_PENALTY=0.093026
PRIMER_RIGHT_1_PENALTY=1.016986
PRIMER_LEFT_1_SEQUENCE=ACCACCTTACCACCACCATAAC
PRIMER_RIGHT_1_SEQUENCE=AACTTGAGTCATTGGCTTCAGA
PRIMER_LEFT_1=126,22
PRIMER_RIGHT_1=361,22
PRIMER_LEFT_1_TM=59.907
PRIMER_RIGHT_1_TM=58.983
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=1.1100
PRIMER_LEFT_2_PENALTY=0.093026
PRIMER_RIGHT_2_PENALTY=1.016986
PRIMER_LEFT_2_SEQUENCE=ACCACCTTACCACCACCATAAC
PRIMER_RIGHT_2_SEQUENCE=TGAGAACTTGAGTCATTGGCTT
PRIMER_LEFT_2=126,22
PRIMER_RIGHT_2=365,22
PRIMER_LEFT_2_TM=59.907
PRIMER_RIGHT_2_TM=58.983
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=240
PRIMER_PAIR_PENALTY_3=1.1100
PRIMER_LEFT_3_PENALTY=0.093026
PRIMER_RIGHT_3_PENALTY=1.016986
PRIMER_LEFT_3_SEQUENCE=ACCACCTTACCACCACCATAAC
PRIMER_RIGHT_3_SEQUENCE=CTTGAGAACTTGAGTCATTGGC
PRIMER_LEFT_3=126,22
PRIMER_RIGHT_3=367,22
PRIMER_LEFT_3_TM=59.907
PRIMER_RIGHT_3_TM=58.983
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=242
PRIMER_PAIR_PENALTY_4=1.2228
PRIMER_LEFT_4_PENALTY=0.093026
PRIMER_RIGHT_4_PENALTY=1.129727
PRIMER_LEFT_4_SEQUENCE=ACCACCTTACCACCACCATAAC
PRIMER_RIGHT_4_SEQUENCE=GGCCTTCCAGTCTTGAGAACTT
PRIMER_LEFT_4=126,22
PRIMER_RIGHT_4=378,22
PRIMER_LEFT_4_TM=59.907
PRIMER_RIGHT_4_TM=61.130
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=253
=
PRIMER_SEQUENCE_ID=SSR_06116 Ps_contig_mira-and-tgicl-ass_franssen_41746
SEQUENCE=AAGCAGATTCATGCAAACTCTGAGGAACAGGAGTAGAAGCAAAAAGATGATCCGAAGAAGAAGGAGCATCGTTGGAAAGACCTTGCATCCTCTTCAAATAAAGTCTATACTTCTGAAGATGACTAGCAACATTCTCACGTGTTAACCCTTCCACATTCATCAACTGCATAATCGTTTCGGAACCGCGTTTTTTATCCCGAGATGAGCCACAACGTCGACGAATCTCTTGTGAAGCTGCGGGGTCCACACTAAACGCGGCCGTTTCAGCGTCGTTTCTGTTCTAACCGCCGAATCCGCTTCCTCCGTAGCGGAATCGATCTTCCGAAGCTTNCGCGAATCCGAACCGGAACCGTCCCGATCGGTTTCCTCTTCCATTTCTTCATCTTCTTCTTCTATAATTTCTTCGTTGTTGG
TARGET=383,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06117 Ps_contig_mira-and-tgicl-ass_franssen_41753
SEQUENCE=AAGCAGTGGTTATCAACGGCAGAGTACGGCGGGGATCTCATTCACACAATCAAACACTCTTTCTTCTTCCATGTTCTACAATGGAGATCCAAAGAATGAAACAGTTCACACTCTTATGGTGTAGAACCCGAAGCAACCGTGTTGCTCTTGTGGGTGGAAACCACACCGCTTCCAGATTTTGCAGTCGCTAAAGTAAAGTTCACATCTTTTTACATTTCTTCTCATCAATCTTCACTTTTCTTCATCTGGGTCGTCTTTGTTTTGATCCATTTAACCAATTTCAATCTCGCTTCGCCTCTGTACTCAGTGTTTCTCAAAAGGGTCTCATTCTGTTCAACATGAAGAAGAAGCATTTTCATGGTGGAACAAGAATTGCTTTGAATCCTCATAATTCTATTGTTATCAGGATTCCTGATACTAAGGTTTTACGCATTTTGTCTCGATCATTGTTCTTGGTTATGGTTCTTGTCGCATTGCCTTTCATAGGTAACATTCTTAATGGATTCTCTTTCACACTGAGTTCCCATTCATTTGTTGTTTCCAAAATTGAATTCTTACATGATATGGCTGAGAAAGGAATCTACAGAAACGATGATAAGGCTCTCATTGTAAGCAGTCCTCCCTATAGCTTTGGATTTGAGGGTATTGATGTGGTCATGGATAATGATTATGAGAGAAAGAGTTTGTTTCTTGATGAGTCCTATGATTTTGTTTTTACATCAAACTCTAGTGATGTCGAATTTGTAGATCGGATTGTAAAAATTGGTGGTATTGTGGTGATGCTGAATTCTTTGAGAGATAAACCATCAAAGTGTGCTTTTAAAGAACAATTACATTATAGAGTTGTTTACCTTAGAAGTTATGGAAGTTCTATGATTGTAGCATTGAGGAAAACTAGCTCGGCAATTAAACACCAATATGGAACTTGAGTTGTGTTTGTTTTGGTTGTGTACTTAGAAGAAGTGCATCATGTGTCACTAATGGCGCACGTTTGTATTCATTTTCCATGTTGTGCTAGTATGATATGATATGATGATGATGATAACACTATAAATAAGCTCATCAGCACATACGATATTGTTGAGCTAGATATTTTGTATATGAATTTTATTTAGTTATTATCAATCCTTTGATATGATRTATTTTTATGGCGTA
TARGET=1019,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06118 Ps_contig_mira-and-tgicl-ass_franssen_41753
SEQUENCE=AAGCAGTGGTTATCAACGGCAGAGTACGGCGGGGATCTCATTCACACAATCAAACACTCTTTCTTCTTCCATGTTCTACAATGGAGATCCAAAGAATGAAACAGTTCACACTCTTATGGTGTAGAACCCGAAGCAACCGTGTTGCTCTTGTGGGTGGAAACCACACCGCTTCCAGATTTTGCAGTCGCTAAAGTAAAGTTCACATCTTTTTACATTTCTTCTCATCAATCTTCACTTTTCTTCATCTGGGTCGTCTTTGTTTTGATCCATTTAACCAATTTCAATCTCGCTTCGCCTCTGTACTCAGTGTTTCTCAAAAGGGTCTCATTCTGTTCAACATGAAGAAGAAGCATTTTCATGGTGGAACAAGAATTGCTTTGAATCCTCATAATTCTATTGTTATCAGGATTCCTGATACTAAGGTTTTACGCATTTTGTCTCGATCATTGTTCTTGGTTATGGTTCTTGTCGCATTGCCTTTCATAGGTAACATTCTTAATGGATTCTCTTTCACACTGAGTTCCCATTCATTTGTTGTTTCCAAAATTGAATTCTTACATGATATGGCTGAGAAAGGAATCTACAGAAACGATGATAAGGCTCTCATTGTAAGCAGTCCTCCCTATAGCTTTGGATTTGAGGGTATTGATGTGGTCATGGATAATGATTATGAGAGAAAGAGTTTGTTTCTTGATGAGTCCTATGATTTTGTTTTTACATCAAACTCTAGTGATGTCGAATTTGTAGATCGGATTGTAAAAATTGGTGGTATTGTGGTGATGCTGAATTCTTTGAGAGATAAACCATCAAAGTGTGCTTTTAAAGAACAATTACATTATAGAGTTGTTTACCTTAGAAGTTATGGAAGTTCTATGATTGTAGCATTGAGGAAAACTAGCTCGGCAATTAAACACCAATATGGAACTTGAGTTGTGTTTGTTTTGGTTGTGTACTTAGAAGAAGTGCATCATGTGTCACTAATGGCGCACGTTTGTATTCATTTTCCATGTTGTGCTAGTATGATATGATATGATGATGATGATAACACTATAAATAAGCTCATCAGCACATACGATATTGTTGAGCTAGATATTTTGTATATGAATTTTATTTAGTTATTATCAATCCTTTGATATGATRTATTTTTATGGCGTA
TARGET=1030,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06119 Ps_contig_mira-and-tgicl-ass_franssen_41778
SEQUENCE=CCAAGCAAATTTTCCATCATTTATTCATTTTCTCCTCTCTTTCTCTCCGTTCTTCCTCCGAAACCAGTTTCATAAACCCTTTCCCTCTTCAATCCTCTTCCCACCCCTTTCATTTTCCTTTCAAATTCTTCTTCTTCAGCCATGTCCATCTATAGGGTACTCTCTCGAGCTTCCAGAAGCTTCACGATTCTCTCTGCGTTGCAGAAGCCACAACCCCTCTCCACCACTTTCTTCCATCATTTCAGTTCCTTTCCTCATCAATCGGCTAGTAAGCTTATTCCGATTCAAGCCAATTTGTTTTGTCCTTCAATAAGTTCATCCTTGACTCCAAGATTTGGATTTTCTACCTCAGCCTCATCGGAGTCTTCAAAAACAAGTGAAGAGGCTAATGCAACTGATCGGAATGAAGAAGATAATGGTGAAGATCAAACGAAGGAATCAGATGCAGAGATTGAATGTGATCTATCCAAAGATGAGTTAATAAAGCTTGTTACTGAGAAAGAAGCACTTTTGAAGTTGAAGCACAAAGAGATTGAAAAAATGCAGGATAAAGTTTTACGTACTTATGCAGAGATGGAGAATGTCATGGACAGGACTAAGCGCAATGCAGAGAATTNCAAAAAAATTTGCTATACAGAATTTCGCCAAGAGTTTACTAGATGTTGCTGACAATCTCGGAAGAGCTTCTTCAGTTGTAAAGGACAGTTTTTACAAAATTGAATCACCTAAAGANCTAGATGAAACTGCACCACTCCTAGAAAACGTCTTCGTGGGAAGGTTGTTAGAAAT
TARGET=126,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2143
PRIMER_LEFT_PENALTY=0.061184
PRIMER_RIGHT_PENALTY=0.153161
PRIMER_LEFT_SEQUENCE=TCATAAACCCTTTCCCTCTTCA
PRIMER_RIGHT_SEQUENCE=TCTGGAAGCTCGAGAGAGTACC
PRIMER_LEFT=69,22
PRIMER_RIGHT=176,22
PRIMER_LEFT_TM=59.939
PRIMER_RIGHT_TM=60.153
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=108
PRIMER_PAIR_PENALTY_1=0.2218
PRIMER_LEFT_1_PENALTY=0.068602
PRIMER_RIGHT_1_PENALTY=0.153161
PRIMER_LEFT_1_SEQUENCE=CCTTTCCCTCTTCAATCCTCTT
PRIMER_RIGHT_1_SEQUENCE=TCTGGAAGCTCGAGAGAGTACC
PRIMER_LEFT_1=77,22
PRIMER_RIGHT_1=176,22
PRIMER_LEFT_1_TM=60.069
PRIMER_RIGHT_1_TM=60.153
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=0.2896
PRIMER_LEFT_2_PENALTY=0.068602
PRIMER_RIGHT_2_PENALTY=0.220992
PRIMER_LEFT_2_SEQUENCE=CCTTTCCCTCTTCAATCCTCTT
PRIMER_RIGHT_2_SEQUENCE=AAATGATGGAAGAAAGTGGTGG
PRIMER_LEFT_2=77,22
PRIMER_RIGHT_2=241,22
PRIMER_LEFT_2_TM=60.069
PRIMER_RIGHT_2_TM=60.221
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=165
PRIMER_PAIR_PENALTY_3=0.3145
PRIMER_LEFT_3_PENALTY=0.061184
PRIMER_RIGHT_3_PENALTY=0.253332
PRIMER_LEFT_3_SEQUENCE=TCATAAACCCTTTCCCTCTTCA
PRIMER_RIGHT_3_SEQUENCE=CGATGAGGCTGAGGTAGAAAAT
PRIMER_LEFT_3=69,22
PRIMER_RIGHT_3=359,22
PRIMER_LEFT_3_TM=59.939
PRIMER_RIGHT_3_TM=59.747
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=291
PRIMER_PAIR_PENALTY_4=0.3359
PRIMER_LEFT_4_PENALTY=0.061184
PRIMER_RIGHT_4_PENALTY=0.274705
PRIMER_LEFT_4_SEQUENCE=TCATAAACCCTTTCCCTCTTCA
PRIMER_RIGHT_4_SEQUENCE=GAGGCTGAGGTAGAAAATCCAA
PRIMER_LEFT_4=69,22
PRIMER_RIGHT_4=355,22
PRIMER_LEFT_4_TM=59.939
PRIMER_RIGHT_4_TM=59.725
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=287
=
PRIMER_SEQUENCE_ID=SSR_06120 Ps_contig_mira-and-tgicl-ass_franssen_41779
SEQUENCE=GTCGGGGATTGCACAAAAATATTCATTCAGTTCCNACTGCACAAAACCCTGCCACAACCACTCACTCACTGCCAACACAACTACTGTATCCACCATTTCTCCTTTCTTTTCAGATCTAACACAAAAAATGCAAACTCATCTCACACTCACTACTATCTTCCTCTACTTCTTCTTAACTCTCTCCACCTCCATCTCAGCCCATGATCAACCCCAACCCCCAACCCCAAGATCTAGTCCGTTCATCATGCCTTCACGCTAGATACCCAAATTTATGTCTCCGAACACTCTCCACCTACACCCGGCCCCG
TARGET=59,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4800
PRIMER_LEFT_PENALTY=0.469712
PRIMER_RIGHT_PENALTY=0.010294
PRIMER_LEFT_SEQUENCE=TTCAGTTCCNACTGCACAAAAC
PRIMER_RIGHT_SEQUENCE=AGGCATGATGAACGGACTAGAT
PRIMER_LEFT=25,22
PRIMER_RIGHT=249,22
PRIMER_LEFT_TM=60.470
PRIMER_RIGHT_TM=59.990
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.50
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.5081
PRIMER_LEFT_1_PENALTY=0.469712
PRIMER_RIGHT_1_PENALTY=0.038361
PRIMER_LEFT_1_SEQUENCE=TTCAGTTCCNACTGCACAAAAC
PRIMER_RIGHT_1_SEQUENCE=GATGAACGGACTAGATCTTGGG
PRIMER_LEFT_1=25,22
PRIMER_RIGHT_1=243,22
PRIMER_LEFT_1_TM=60.470
PRIMER_RIGHT_1_TM=59.962
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.50
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=219
PRIMER_PAIR_PENALTY_2=0.5990
PRIMER_LEFT_2_PENALTY=0.469712
PRIMER_RIGHT_2_PENALTY=0.129335
PRIMER_LEFT_2_SEQUENCE=TTCAGTTCCNACTGCACAAAAC
PRIMER_RIGHT_2_SEQUENCE=CTGAGATGGAGGTGGAGAGAGT
PRIMER_LEFT_2=25,22
PRIMER_RIGHT_2=196,22
PRIMER_LEFT_2_TM=60.470
PRIMER_RIGHT_2_TM=59.871
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=5.50
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=4.75
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=0.7220
PRIMER_LEFT_3_PENALTY=0.469712
PRIMER_RIGHT_3_PENALTY=0.252279
PRIMER_LEFT_3_SEQUENCE=TTCAGTTCCNACTGCACAAAAC
PRIMER_RIGHT_3_SEQUENCE=TGAGATGGAGGTGGAGAGAGTT
PRIMER_LEFT_3=25,22
PRIMER_RIGHT_3=195,22
PRIMER_LEFT_3_TM=60.470
PRIMER_RIGHT_3_TM=60.252
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=5.50
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.75
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=171
PRIMER_PAIR_PENALTY_4=0.7354
PRIMER_LEFT_4_PENALTY=0.469712
PRIMER_RIGHT_4_PENALTY=0.265715
PRIMER_LEFT_4_SEQUENCE=TTCAGTTCCNACTGCACAAAAC
PRIMER_RIGHT_4_SEQUENCE=TGGAGAGTGTTCGGAGACATAA
PRIMER_LEFT_4=25,22
PRIMER_RIGHT_4=290,22
PRIMER_LEFT_4_TM=60.470
PRIMER_RIGHT_4_TM=59.734
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.50
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=3.75
PRIMER_PAIR_4_COMPL_END=0.75
PRIMER_PRODUCT_SIZE_4=266
=
PRIMER_SEQUENCE_ID=SSR_06121 Ps_contig_mira-and-tgicl-ass_franssen_41792
SEQUENCE=TCTTCTTCTTCTTCCCATTCATTTTCCCGCGTAAAACCGTTACTTCCACCGCCGAACTTCAACTCCCGTTCATCATCCTCTTCACTATTAATCAAATTCAAAACCCGTAATTTTGGCATCGACGTCTTGCAATCGGAGTCCAACCGTAGTTTCGTCACACAATCAATCGGAGGGAATTTGAATTTGAGTTTCCCTCTGATTTCACCTAACGACCAATGGGGAAACTGGACCGCTCTATTCGCCGCCGCGGCATTCGGAATCTGGTCAGAGAAAACGGAGATCGGGAAGACAGTGAGTGGAGCCATTGTGACTATTTTGGTGTGTCTTGCTGCTAGTAAATTTGGGGATTCTTTCCGTTGATGCTCCTG
TARGET=1,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06122 Ps_contig_mira-and-tgicl-ass_franssen_41796
SEQUENCE=CGCGGGGGGGARTCAAACCACCGCCGCAAACCATTCACTCTTCCTAAGCCCTTTGTCGTGTCCTTTTTATTTCACCATTATTACTACTATCTTGTTAATGTCTTAAAACACTTTATTACTCCCTTTAAAATACAATTACTCTAATCCTAGTACCTTCATATATCTTTGAGAGAGACACAGAAAAGAGACAGATCTATCAGATTTAAGAATTCTCAACAACAAGATGCATGCAAAGACAGACTCCGAGGTTACTAGCCTTGACGCATCTTCCACCACAAGATCCCCTCGTCGACAAGCATACTATGTTCAAAGTCCATCTCACGATGGTGAAAAAACAACTACCTCCTTCCACTCAACTCCTGTCATCAGTCCAATGGGTTCTCCGCCTCATTCCCACTCTTCCTCTAGCCGTTTCTCCGGTTCCCGCAAAATCAACAACAACCACCGTAACGCCAAACCTTGGAAAGACATCGATGTTATTGAAGAAGAGGGTCTTCTTCAAAACCAAGATCATAACCGTTCTCTCTCTCGTCGTTACTATTTCCTAGCTTTTATTCTTGGCTTCTTTCTTTTGTTCACTCTTTTCTCTCTTATCCTTTGGGGTGCTAGTAGACCCATGAAACCCCATGTCGTAATTAAGAGTATAAAATTCGATCATGTTAGAGTTCAAGCTGGTTCTGATGCAACCGGTGTGGCTACTGATATGATCACAGTGAACTCAACTGTCAAATTCACATATCGGAACAAAGGCACATTCTTTGGTGTTCATGTTTCGTCCACGCCTTTAGATCTATCCTATTCAGATATTGTAATTGGCACCGGCGATATGAAGAAATTTTATCAATCAAGGAAAAGTCAAAGATTAGTGAGTGTAGCAGTGATGGGAAGCAAGATCCCATTGTATGGAAGTGGTGCTAGTCTAAGTAGCACAACAGGTATGCCAAGTTTACCAGTGCCATTGAAATTGAGCTTTGAGATTAGATCAAGAGCTTATGTGCTTAGGAAAATTNGNGTAAACNAAAATACYCAAAATAACCTAACAAGTAAGTTC
TARGET=521,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06123 Ps_contig_mira-and-tgicl-ass_franssen_41800
SEQUENCE=ATTTGAAAAATAGCTGGAGTCCTAACACTATTTTTTCCGTTCCTTTTTCCGGCAACGAAGAACCAAACCTTGCACTCATACCGCTATGAGTAAGACTTTTCTCACCATCAGCCCAATTTTCCACCTTAACCAATTGGAAGGGGTTATTGCATGATTCGGATTTTGGAGCTCTTTCGTCATCGCGCATCACATCATCGGAGGCAGATGCAGTCAGAACNGCATGAGCAATACAGAANGCGGAGAGAGAGAAGAAGAGAGNGCTNNTGTGTGGTAT
TARGET=240,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06124 Ps_contig_mira-and-tgicl-ass_franssen_41825
SEQUENCE=TGATAYTTGTAACTAAACAAACAATGATTGTACTTGATATTAATGTTTTACATGCACAAGGTCTCGTTCTATGGTGCTGATATCCGATCAAGGCACAAATTGAGTAAAAAGTTTAACAGAAATAAAAATGTTGAATCCACCCTTTTCATCTTTTCTTAAAACCATACTTCTTTCGTTCGTAGAATAAATCAGTTTTGGCACTACCTAAGTTAACAATCTTGGGGCAACCATCCCTATCTTTCTCCTGCTGTGTGCATTCCTTGCAGTAGTATGCATCTGAAATTCCTACTCCTCCGCATATGACACATCGACCTTGAAACGATCCATAGTTGCACTCATCGCAGACCCTGACAAGTGTACATGGACGCACATAAGAGTCGCATATCACACACTTGCCGTCACATTTCTCACACAATCGTCCGATAGCAATTCCTGGCTGCTTCCGGCACATGATCAAATCAGGATGATGTTTGGCCATGTTTTCAACAACCTGCTAGATGCTGTGGTGAGTAGAAGAGTGGTGTTAAACGATGTCGTTTTGCCTGAACTAAGAACCTTGTGCGGTGGCGGAGGTGATGAAAAAGAGAGAGAACNNNNGAAAGAAAGAAAAAAGTAAC
TARGET=598,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06125 Ps_contig_mira-and-tgicl-ass_franssen_41833
SEQUENCE=ATGGGACTCAGCCATCATATCCTTCGGATCCCTCATTATTTTCTGACAGGCAGTCCAAACATGGAGCAAAATTTGGATTATCTTCGCCAGCTATGCCTGCCAAAGATTACAACTCTCAAAGGAATACCAATTTTCCCCAGCCACTTCCACAATTCACAAACATGTATGCTTCCATGCATTCATCTGGACTGGGTCTGTCTTCTGGATATGCTAATGGGATGTACCCAAGCAACACGGCGTACAGCCAATATGGAAACACATACAGAGCTAGGTCAAATTTTGGATCTACTGCATATGGATCCAGAGCAGGTCCTTTTGATAACAAGTTTAAAACCTCAGGTTATGGCCGAGGTTTTGACCATGTCAAAAAAAACACAGATGGCTTTGGTGAGCTGAACAAGGGACCAAGAGGTTCCAACAGTTCTGATGATAAAAATGTCAAAAGTCTTGGACCTGTCACATTATTGCTCAAGGGTCAGGACCTTCCTGTCAAGAGTGATGATGRTAAAGAAGTGCATCCAGTTCCCAACAAGCAACATTACAACGGGGAAGATTTATCTGAGAGCTATTCTGATGCAAAGTTTTATGTCATCAAATCTTATAGTGAGGATGATATTCATAAAAGCATGAAATATAATGTGTGGACAARCACCCCCTAATGGCAACAAGAAGCTAGATGCAGCATATCAGGAAGCCAAGGAGAAGCCTGGTGACTGTCCCATATTTTTGCTATTTTCGGTCAATACTAGTGGACAGTTTGTTGGTTTGGCAGAAATGGTGAGTCCAGTTGATTTTGACAGAACAGTAGAGTATTGGCAGCAGGACAGGTGGACTGGTTGCTTTTCTGTGAAGTGGCATATCATAAAGGATATCCCAAATAATGTGTTGAGACACATAACGCTAGAAAACAATGAATACAAGCCTGTTACAAACAGTAGGGACACTCAAGAGGTCAAGTTTGAGAAGGGTATTGAAATTCTCAAAATCTTCAAGGAGTATTCAAGCAAAACATGCATTCTGGATGATTTTGGATTTTACGAGGCTCGTGAAAAGACAACTCAGGAGAAAAAAGCCAAGGAACAATTGCTCACAAAAGAGACGGTCAGCAAGTCCAATGATTTAACAACTGTTGGAACAGACATGTTGCCTAAGTCCCATGATGGAACTTTGACAAATGAATCAGTTATTCCGGATGTAGCAGAAACAAGGGAGAATCTTACAGAAGTGAATGGCGGACCTACTTCTACTGAACCACCTGCAGACTCTTTGAATAGCTGCTTAACCGGTGACAAAATCGGTGACAAAGTCAAGCCTTAGAGTGGTTAAAATGTGATTTTCAATCCCCCTCCAAACCTTTTATCCTTTTTCTTGAATTACTTCAGTATGGAGAAAGTTTTTGTGTTTTTTTTAATTGCTTGACCTAGTTTGTTTGACCTTAATCTTTTAGTGGAGTATATCAGTTTATCTTTTTCTTAGATAGTCTAATAGGATGTCAAGACTTGATATTATGTGTGTGTGAGAGTGGCCTCTCTAATCCATGAAGATATATTTTCTAATGATGACAGTATTGCTGAGAATGAAAAACATGCTTGATTTAGTTTTTGTAGGATTTAGTGATTTATATGTTCT
TARGET=1509,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06126 Ps_contig_mira-and-tgicl-ass_franssen_41836
SEQUENCE=GTAAAGAGTNGATAAAAATTTCAAGAAATATATCTTCAAACAGAAGTTTACATTACAAACTGAAAACAGAATCAACCCTTGTCCCAAAACCAAGGAAGAAAGAAAGAAAACACAGACACTTCTTGTCACAACAGTAGTTTGTGTCTTTCGTTGCAATTACCTTCCTCCTCCTCCAAAAAAATCCATTAGTAGTACCTGGGTAGAACACATTCATGTAAAGCCTAGTGTTAATCTAACTCGACTCAGATTCACTGTACCGAGTCAATCTAAGTTGTTGGATAGAGATTCCAATTCAAGGACTCGTGTCGTCGAATCGACAACTTGAAACCTCAAAGTGTCCAATTTTTATAACATCTAGCCCACAGCATCATATGCATTCCCATACTAACAACCCAAACAACCAAGATTGGAAAAAACAAATTAAGATAACAAAAACAGAAGCCAAAAAACATGCCTATTACAAAACACATCGATCACAACATGAACCTGAATCATACTACGGTACTTATTATTTACAAAGAGAGAATTGAAATTCAACATTGAAAACACAAAAACCCAACACAACTAACTGAGAGCAGAAAAACCACCACCCTTCATCATCTGCTTAAACTCCTTATAATCAACCAACCCATCTCCGTCAACATCAACCTTCCCTATCATCTTCTTACAATCTTCCACAGTTCTTCCCTGCTTCAATCCCAATGACACCAAAACAGATCTCAACTCATCCACACTAATAAACCCGTCTCCATTTTGATCAAACACATTGAATGCTTCCCTCATATCTTCCTCTTCCTCTTCAACCCGTTCATCCATAATCGACTCATACAGCTCTCTAAACTCCTCAATATCCACACACCCGTCTCGGTTCACATCGATCTTCTCGATCATCTGACTCAGCTCTTTGTCAGGGATGAAAATTCCTAAATTCTCCAACGAATCGTTCAGCTCCTTCTTCGTTATTCGACCGTCATCGTTGCGGTCAAACATCTGGAAAACACGCTTGAGTTCGGTTGGATCCATTGTTGTTGTCTCGGTAATAACAAGACCTCTTTTCGATGATGAAAGTGTAGTAGTAGTGTTGTTTTGGATGATGAGATTGGTTAAGGATTGATGGGAGAACCAAGAAAGTGGGAAGAAGGTTATAAGTTTCTTAGGAACAAGAGAAATGAGAAATGAATTGACAACGTTGTATAGAAGGAAAATCCTAAGCAAGATAGTTGGCATTTGAAACAAGGAAACTAATGTGTGATCCCNTNTCTTGTTGGTCCTTTGTTGAATATAAAAGGAAAGTTTGGAGAAATTAAAGTAGTAACAACTACTAGGAAGAGAGAATTGAGTGTTTCTTTTTTGGGTCGGGTTTTAAGATTGAGGTGGTCCCCGAC
TARGET=96,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9311
PRIMER_LEFT_PENALTY=0.629225
PRIMER_RIGHT_PENALTY=0.301915
PRIMER_LEFT_SEQUENCE=AAAACAGAATCAACCCTTGTCC
PRIMER_RIGHT_SEQUENCE=TTTGGAGGAGGAGGAAGGTAAT
PRIMER_LEFT=62,22
PRIMER_RIGHT=176,22
PRIMER_LEFT_TM=59.371
PRIMER_RIGHT_TM=60.302
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=0.9417
PRIMER_LEFT_1_PENALTY=0.629225
PRIMER_RIGHT_1_PENALTY=0.312496
PRIMER_LEFT_1_SEQUENCE=AAAACAGAATCAACCCTTGTCC
PRIMER_RIGHT_1_SEQUENCE=TCGGTACAGTGAATCTGAGTCG
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=259,22
PRIMER_LEFT_1_TM=59.371
PRIMER_RIGHT_1_TM=60.312
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=198
PRIMER_PAIR_PENALTY_2=0.9625
PRIMER_LEFT_2_PENALTY=0.629225
PRIMER_RIGHT_2_PENALTY=0.333229
PRIMER_LEFT_2_SEQUENCE=AAAACAGAATCAACCCTTGTCC
PRIMER_RIGHT_2_SEQUENCE=AGGAAGGTAATTGCAACGAAAG
PRIMER_LEFT_2=62,22
PRIMER_RIGHT_2=165,22
PRIMER_LEFT_2_TM=59.371
PRIMER_RIGHT_2_TM=59.667
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=104
PRIMER_PAIR_PENALTY_3=1.0994
PRIMER_LEFT_3_PENALTY=0.629225
PRIMER_RIGHT_3_PENALTY=0.470203
PRIMER_LEFT_3_SEQUENCE=AAAACAGAATCAACCCTTGTCC
PRIMER_RIGHT_3_SEQUENCE=GGAAGGTAATTGCAACGAAAGA
PRIMER_LEFT_3=62,22
PRIMER_RIGHT_3=164,22
PRIMER_LEFT_3_TM=59.371
PRIMER_RIGHT_3_TM=60.470
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=103
PRIMER_PAIR_PENALTY_4=1.0994
PRIMER_LEFT_4_PENALTY=0.629225
PRIMER_RIGHT_4_PENALTY=0.470203
PRIMER_LEFT_4_SEQUENCE=AAAACAGAATCAACCCTTGTCC
PRIMER_RIGHT_4_SEQUENCE=GAGGAAGGTAATTGCAACGAAA
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=166,22
PRIMER_LEFT_4_TM=59.371
PRIMER_RIGHT_4_TM=60.470
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=105
=
PRIMER_SEQUENCE_ID=SSR_06127 Ps_contig_mira-and-tgicl-ass_franssen_41836
SEQUENCE=GTAAAGAGTNGATAAAAATTTCAAGAAATATATCTTCAAACAGAAGTTTACATTACAAACTGAAAACAGAATCAACCCTTGTCCCAAAACCAAGGAAGAAAGAAAGAAAACACAGACACTTCTTGTCACAACAGTAGTTTGTGTCTTTCGTTGCAATTACCTTCCTCCTCCTCCAAAAAAATCCATTAGTAGTACCTGGGTAGAACACATTCATGTAAAGCCTAGTGTTAATCTAACTCGACTCAGATTCACTGTACCGAGTCAATCTAAGTTGTTGGATAGAGATTCCAATTCAAGGACTCGTGTCGTCGAATCGACAACTTGAAACCTCAAAGTGTCCAATTTTTATAACATCTAGCCCACAGCATCATATGCATTCCCATACTAACAACCCAAACAACCAAGATTGGAAAAAACAAATTAAGATAACAAAAACAGAAGCCAAAAAACATGCCTATTACAAAACACATCGATCACAACATGAACCTGAATCATACTACGGTACTTATTATTTACAAAGAGAGAATTGAAATTCAACATTGAAAACACAAAAACCCAACACAACTAACTGAGAGCAGAAAAACCACCACCCTTCATCATCTGCTTAAACTCCTTATAATCAACCAACCCATCTCCGTCAACATCAACCTTCCCTATCATCTTCTTACAATCTTCCACAGTTCTTCCCTGCTTCAATCCCAATGACACCAAAACAGATCTCAACTCATCCACACTAATAAACCCGTCTCCATTTTGATCAAACACATTGAATGCTTCCCTCATATCTTCCTCTTCCTCTTCAACCCGTTCATCCATAATCGACTCATACAGCTCTCTAAACTCCTCAATATCCACACACCCGTCTCGGTTCACATCGATCTTCTCGATCATCTGACTCAGCTCTTTGTCAGGGATGAAAATTCCTAAATTCTCCAACGAATCGTTCAGCTCCTTCTTCGTTATTCGACCGTCATCGTTGCGGTCAAACATCTGGAAAACACGCTTGAGTTCGGTTGGATCCATTGTTGTTGTCTCGGTAATAACAAGACCTCTTTTCGATGATGAAAGTGTAGTAGTAGTGTTGTTTTGGATGATGAGATTGGTTAAGGATTGATGGGAGAACCAAGAAAGTGGGAAGAAGGTTATAAGTTTCTTAGGAACAAGAGAAATGAGAAATGAATTGACAACGTTGTATAGAAGGAAAATCCTAAGCAAGATAGTTGGCATTTGAAACAAGGAAACTAATGTGTGATCCCNTNTCTTGTTGGTCCTTTGTTGAATATAAAAGGAAAGTTTGGAGAAATTAAAGTAGTAACAACTACTAGGAAGAGAGAATTGAGTGTTTCTTTTTTGGGTCGGGTTTTAAGATTGAGGTGGTCCCCGAC
TARGET=163,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3517
PRIMER_LEFT_PENALTY=0.039178
PRIMER_RIGHT_PENALTY=0.312496
PRIMER_LEFT_SEQUENCE=TGTGTCTTTCGTTGCAATTACC
PRIMER_RIGHT_SEQUENCE=TCGGTACAGTGAATCTGAGTCG
PRIMER_LEFT=139,22
PRIMER_RIGHT=259,22
PRIMER_LEFT_TM=60.039
PRIMER_RIGHT_TM=60.312
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=121
PRIMER_PAIR_PENALTY_1=0.4192
PRIMER_LEFT_1_PENALTY=0.039178
PRIMER_RIGHT_1_PENALTY=0.380020
PRIMER_LEFT_1_SEQUENCE=TGTGTCTTTCGTTGCAATTACC
PRIMER_RIGHT_1_SEQUENCE=TATGATGCTGTGGGCTAGATGT
PRIMER_LEFT_1=139,22
PRIMER_RIGHT_1=371,22
PRIMER_LEFT_1_TM=60.039
PRIMER_RIGHT_1_TM=59.620
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=233
PRIMER_PAIR_PENALTY_2=0.6969
PRIMER_LEFT_2_PENALTY=0.039178
PRIMER_RIGHT_2_PENALTY=0.657742
PRIMER_LEFT_2_SEQUENCE=TGTGTCTTTCGTTGCAATTACC
PRIMER_RIGHT_2_SEQUENCE=TGATGCTGTGGGCTAGATGTTA
PRIMER_LEFT_2=139,22
PRIMER_RIGHT_2=369,22
PRIMER_LEFT_2_TM=60.039
PRIMER_RIGHT_2_TM=60.658
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=231
PRIMER_PAIR_PENALTY_3=0.7289
PRIMER_LEFT_3_PENALTY=0.039178
PRIMER_RIGHT_3_PENALTY=0.689754
PRIMER_LEFT_3_SEQUENCE=TGTGTCTTTCGTTGCAATTACC
PRIMER_RIGHT_3_SEQUENCE=TATGGGAATGCATATGATGCTG
PRIMER_LEFT_3=139,22
PRIMER_RIGHT_3=383,22
PRIMER_LEFT_3_TM=60.039
PRIMER_RIGHT_3_TM=60.690
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.7812
PRIMER_LEFT_4_PENALTY=0.039178
PRIMER_RIGHT_4_PENALTY=0.742018
PRIMER_LEFT_4_SEQUENCE=TGTGTCTTTCGTTGCAATTACC
PRIMER_RIGHT_4_SEQUENCE=TTGGACACTTTGAGGTTTCAAG
PRIMER_LEFT_4=139,22
PRIMER_RIGHT_4=341,22
PRIMER_LEFT_4_TM=60.039
PRIMER_RIGHT_4_TM=59.258
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_06128 Ps_contig_mira-and-tgicl-ass_franssen_41840
SEQUENCE=TTTATTTGTTTATATTTGTCTGCCAAGAATGGTTGACTTTTTATAGATCTGAGTGTTTGTTTGTTGTTCTTATTGAGAACTGAGGAAGGTTCAGGCACATGATATTTTGATATCTCTCTTGTGATAGGAAGACCATGGGACGAGCTTCACCATTACTTGTAATATGTTTGGTTCTTGGTTCTTCCTTTGCTACATACAATTTGGTCACAATGATAATCCACTATGGATCGTTAGAAAGTGTAGCTACCGAGGATGGTGGATTGTTTTTTGACCCTATTATCGAGATGCCTGAACATGTGAAGAACCGAAAAACATCTAAAGTACCGTTTCATATTGCGCTGACGGCTACTGATGCTGTGTACAGCAAATGGCAATGCCGCATCATGTATTACTGGTATAAGAAACAAAGGAATTTACCCGGGTCAGAGATGGGAGGATTCACTAGAATTTTGCATTCTGGAAAGGCTGACAACTTGATGGATGAAATTCCTACTTTTGTTGTTAATCCTCTTCCGAGAGGTCTAGACCGGGGATACATCGTTCTGAATAGACCATGGGCTTTTGTTCAGTGGCTGCATACGGCTAAAATAGAGGAAGAATATATTTTAATGGCAGAACCTGATCACGTATTTGTACGGCCTCTTCCCAATCTGGCGTATGGAGCACATCCAGCTGCTTTTCCATTTTTTTATATTAAGCCAGAGCAGAACGAAAAAATCGTAAGGAAATATTACCCTGAGGAAAAGGGACCGGTTACAAATATTGATCCGATTGGCAATTCTCCTGTAATTATCAGAAAGGATTTGATTTCAAAAATTGCTCCCACATGGATGAATGTTTCACTGGAGATGAAAGAGGATCCAGAGACCGATAAAGAGTTTGGTTGGGTGCTAGAAATGTATGCTTATGCTATTGCATCTGCTCTGCATGGTGTGAAACATATTCTACGGAAAGACTTCATGCTGCAGCCACCGTGGGATCTCGAAACTTACAATAAGTTCATTATTCATTATACTTATGGGTGTGACTACAGTTTAAAGGGTGAATTGACTTACGGTAAAATTGGCGAGTGGCGATTTGACAAAAGGTCACATTTAGCAAGACCTCCACCAAGAGACTTGCCTTTACCTCCTCCAGGTGTTCCTGAAAGTGTGGTGACTCTGGTAAAGATGGTGAATGAAGCTTCTGCTAACATCCCCAATTGGGAGACATTGTAAATATTTTTATTTATTCATTCATGATATTCAAAATGCATGTTTTGATAGTTCTAAAAAAGTGTAGACATCGAGACAATAGTTTATATTTTTCTTCCTTGTTTAGTCATTAGAACCATTGAACAATTTGCACCAAATACCTCACTATACACTTTTAATTAGATGACGATTTAAACGAAGGATTATACTTTGTTTTTCGTAGGTGATGTTCTTGAATGAGACCACAATGCTGAATAATTTACTTYAAGTAATATTGGATTT
TARGET=54,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06129 Ps_contig_mira-and-tgicl-ass_franssen_41862
SEQUENCE=AACGCANAGTACTCGGGGGAGGNAAGAAAGTGTTTTAAAAACGCCCAAAGCAAAAAGCTCCTCCCCTCCCTCCCTCCGATTATTCAGATCTACTCCCCTCCCTCGTAGAAGAAGAAGAAGATGGAGAGCGAAAAGAAATCCTCTTCCATTCAAACTTTGGAGCATGGGGTATGAACGTTGTGAGCTCCGTTGGAATTATCATGGCTAATAAACACCTCATGTCCAATAATGGCTACGCTTTCACCTTCGGTAAGATCTCTCTTTCTCTTTCTCGATCTGCACCA
TARGET=64,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7634
PRIMER_LEFT_PENALTY=0.729328
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=GGGGAGGNAAGAAAGTGTTTTA
PRIMER_RIGHT_SEQUENCE=TTTCTTTTCGCTCTCCATCTTC
PRIMER_LEFT=15,22
PRIMER_RIGHT=137,22
PRIMER_LEFT_TM=59.271
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.50
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.75
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=123
PRIMER_PAIR_PENALTY_1=0.8249
PRIMER_LEFT_1_PENALTY=0.729328
PRIMER_RIGHT_1_PENALTY=0.095574
PRIMER_LEFT_1_SEQUENCE=GGGGAGGNAAGAAAGTGTTTTA
PRIMER_RIGHT_1_SEQUENCE=TTCGCTCTCCATCTTCTTCTTC
PRIMER_LEFT_1=15,22
PRIMER_RIGHT_1=131,22
PRIMER_LEFT_1_TM=59.271
PRIMER_RIGHT_1_TM=60.096
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.50
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.75
PRIMER_PRODUCT_SIZE_1=117
PRIMER_PAIR_PENALTY_2=0.8392
PRIMER_LEFT_2_PENALTY=0.729328
PRIMER_RIGHT_2_PENALTY=0.109882
PRIMER_LEFT_2_SEQUENCE=GGGGAGGNAAGAAAGTGTTTTA
PRIMER_RIGHT_2_SEQUENCE=AAGCGTAGCCATTATTGGACAT
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=239,22
PRIMER_LEFT_2_TM=59.271
PRIMER_RIGHT_2_TM=59.890
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.50
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=0.9944
PRIMER_LEFT_3_PENALTY=0.729328
PRIMER_RIGHT_3_PENALTY=0.265053
PRIMER_LEFT_3_SEQUENCE=GGGGAGGNAAGAAAGTGTTTTA
PRIMER_RIGHT_3_SEQUENCE=TCGCTCTCCATCTTCTTCTTCT
PRIMER_LEFT_3=15,22
PRIMER_RIGHT_3=130,22
PRIMER_LEFT_3_TM=59.271
PRIMER_RIGHT_3_TM=59.735
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.50
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=116
PRIMER_PAIR_PENALTY_4=1.0299
PRIMER_LEFT_4_PENALTY=0.729328
PRIMER_RIGHT_4_PENALTY=0.300577
PRIMER_LEFT_4_SEQUENCE=GGGGAGGNAAGAAAGTGTTTTA
PRIMER_RIGHT_4_SEQUENCE=CTTACCGAAGGTGAAAGCGTAG
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=253,22
PRIMER_LEFT_4_TM=59.271
PRIMER_RIGHT_4_TM=60.301
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.50
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=4.75
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_06130 Ps_contig_mira-and-tgicl-ass_franssen_41862
SEQUENCE=AACGCANAGTACTCGGGGGAGGNAAGAAAGTGTTTTAAAAACGCCCAAAGCAAAAAGCTCCTCCCCTCCCTCCCTCCGATTATTCAGATCTACTCCCCTCCCTCGTAGAAGAAGAAGAAGATGGAGAGCGAAAAGAAATCCTCTTCCATTCAAACTTTGGAGCATGGGGTATGAACGTTGTGAGCTCCGTTGGAATTATCATGGCTAATAAACACCTCATGTCCAATAATGGCTACGCTTTCACCTTCGGTAAGATCTCTCTTTCTCTTTCTCGATCTGCACCA
TARGET=107,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5679
PRIMER_LEFT_PENALTY=0.458031
PRIMER_RIGHT_PENALTY=0.109882
PRIMER_LEFT_SEQUENCE=AGATCTACTCCCCTCCCTCGTA
PRIMER_RIGHT_SEQUENCE=AAGCGTAGCCATTATTGGACAT
PRIMER_LEFT=85,22
PRIMER_RIGHT=239,22
PRIMER_LEFT_TM=60.458
PRIMER_RIGHT_TM=59.890
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.6591
PRIMER_LEFT_1_PENALTY=0.549188
PRIMER_RIGHT_1_PENALTY=0.109882
PRIMER_LEFT_1_SEQUENCE=ATTCAGATCTACTCCCCTCCCT
PRIMER_RIGHT_1_SEQUENCE=AAGCGTAGCCATTATTGGACAT
PRIMER_LEFT_1=81,22
PRIMER_RIGHT_1=239,22
PRIMER_LEFT_1_TM=59.451
PRIMER_RIGHT_1_TM=59.890
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=159
PRIMER_PAIR_PENALTY_2=0.8392
PRIMER_LEFT_2_PENALTY=0.729328
PRIMER_RIGHT_2_PENALTY=0.109882
PRIMER_LEFT_2_SEQUENCE=GGGGAGGNAAGAAAGTGTTTTA
PRIMER_RIGHT_2_SEQUENCE=AAGCGTAGCCATTATTGGACAT
PRIMER_LEFT_2=15,22
PRIMER_RIGHT_2=239,22
PRIMER_LEFT_2_TM=59.271
PRIMER_RIGHT_2_TM=59.890
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.50
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=225
PRIMER_PAIR_PENALTY_3=0.8498
PRIMER_LEFT_3_PENALTY=0.549188
PRIMER_RIGHT_3_PENALTY=0.300577
PRIMER_LEFT_3_SEQUENCE=ATTCAGATCTACTCCCCTCCCT
PRIMER_RIGHT_3_SEQUENCE=CTTACCGAAGGTGAAAGCGTAG
PRIMER_LEFT_3=81,22
PRIMER_RIGHT_3=253,22
PRIMER_LEFT_3_TM=59.451
PRIMER_RIGHT_3_TM=60.301
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=173
PRIMER_PAIR_PENALTY_4=0.9617
PRIMER_LEFT_4_PENALTY=0.458031
PRIMER_RIGHT_4_PENALTY=0.503688
PRIMER_LEFT_4_SEQUENCE=AGATCTACTCCCCTCCCTCGTA
PRIMER_RIGHT_4_SEQUENCE=CGTAGCCATTATTGGACATGAG
PRIMER_LEFT_4=85,22
PRIMER_RIGHT_4=236,22
PRIMER_LEFT_4_TM=60.458
PRIMER_RIGHT_4_TM=59.496
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.4000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_06131 Ps_contig_mira-and-tgicl-ass_franssen_41865
SEQUENCE=AAGGCAGAGCATTTTACAGAGTGGCCAATCCTTGAAATCTTTAGAACCTTTAGTCGTCAAAGCAGTGGTGGAGTCACTGGTGGAAAAGTCAGTCGAGGACTTGAGACAGATGAAGGGGATAACAGCGACTTACAGGATGACTAATAAACCTCTACCTGTTAGGCATTCACCATATGTTACAGGAGTATTACGTCCCCTAAAGACTTTTTTGGTATGGGGAAAGAACCTCTAGATATTTGGCAAGTGAAACTAAAAAGGAAATACTCCTTTGTGCTGCAACTGAGATTACTGATCGTTATTATGAATTAGCTGCCGACCTTGTTAGTGTGGCAAGAAAGACAGAATCTTCACTTCAGAAAATTAGACAGAGTGCACAAAGACGAGCGGGGGCAAGTTCAGACATCTCCGATAATAATGTGTCTGACACTGACAAAATGTGTATGCAGTTATTCCTTGATATTCAGGAGTATGCCCGCAACCTATCTTCCCTAGGGGTTGAAGCAGTTAATATTGCATCCTATCGTTCATTATGGCAGTGTGTGGCTCCAGCAGATAAGCAGAACACAATTAAATTATAAGAGTTTTCATCTGTGGAGTCAATGTGGCTTGTAGATTGATTGATTGCTTTCTTGGTTAGCCAAGATTTTCTTGTGTAACTTAACCATAAAATCTAGATTATACAAATTAGTTGGCTTTTACACAATAATCACAGGTTACCATAGTTGTT
TARGET=612,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06132 Ps_contig_mira-and-tgicl-ass_franssen_41871
SEQUENCE=ATTACATGAGTGATGTNGGTCTGAGTAAACCAAACCAAACCAAACCTTTAAACTCATCACATGGTTATGGCCTTATCTTCTTCTTCTTCTCTCATTCTCAACACCACTGATTATTCACCTCACAAACCACTCCATTCACCATCATCCTCCTCATCATCATTTTCTTCTTCTCACTTTCCAATCAAACCTCTCATCCACCACTCTCACAACCTTCCCATATCTCAAAAATGGAGAAGAAATGTTTCATTCTTTACTTCTTTCTTAAAGAAACCCAAAGACGCAAGCACCATTAAGGAAGAGCTTCTTGAAGCCATTGCTTCGCTTGATCGAGGAGCTGATGCCACTCCTGAAGACCAACAAAGCGTTGATCAGATTGCACGCAAACTTGAAGCAGTTAATCCAACAAAGGCGCCACTCAAATCTAGTTTACTTGATGGAAAATGGGAGCTTATTTACACTACCTCTCAGTCAATCTTGCAAACTAAGAGACCAAAAATTTTGAGATCAGTTACAAATTATCAAGCAATCAATGCTGATACTCTCCGAGCCCAAAACATGGAATCTCTGCCATTCTTCAACCAGGTGACAGCAGATTTAACACCTTTGAACACAAGGAAAGTGGCTGTGAAATTCGATACCTTCAAAATTGCAGGCTTCATACCTGTTAAGGCTCCTGACACAGCTCGCGGCGAACTTGAAATAACATATTTGGATGAAGAACTTCGGGTATCAAGAGGGGACAAAGGAAACTTGTTCATCTTGAAAATGGTGGATCCATCTTATCGGATTCCTGCATGATGTGTCGTCACTGTAGCATTCTATCGCTATATATCGTGTTTTTTCCCTCTCAACGGATTCAAACTACTATCAGAGCTTTCTGAAGTTGGAACTTATACTAGCAAGCCAATGCATACCACTACTCAAGCTTAACTTGAAGCATTTTGGAACTGTGCTTTCTGTACAGAAGTGGTTGTAAAATCTTTACATACTGAGAGTTTTAGTGCAAATTGCTTCTTTCAAATATCAGCGGAATTTGATAGTTCTTCATTATGTATTATTTTTAGAGTTCTTTTGAAGTAGTTTTCAACTTAAATAAGTAATATTTTAATAAGTTTTATAGTTAAAAGTAAAGAAAGTAAAAGTCTTGAAACTCTTGAGTTATTTTGTTTGTCTTATTGTTAATTCTTCTTAACAAGAAGTCATTTGTGTAATGCATAAGCTTATTGCCTTTGTAGGTT
TARGET=27,20
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1297
PRIMER_LEFT_PENALTY=2.042650
PRIMER_RIGHT_PENALTY=0.087031
PRIMER_LEFT_SEQUENCE=CATGAGTGATGTNGGTCTGAGT
PRIMER_RIGHT_SEQUENCE=AGCTCTTCCTTAATGGTGCTTG
PRIMER_LEFT=4,22
PRIMER_RIGHT=301,22
PRIMER_LEFT_TM=57.957
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=298
PRIMER_PAIR_PENALTY_1=2.1297
PRIMER_LEFT_1_PENALTY=2.042650
PRIMER_RIGHT_1_PENALTY=0.087031
PRIMER_LEFT_1_SEQUENCE=ACATGAGTGATGTNGGTCTGAG
PRIMER_RIGHT_1_SEQUENCE=AGCTCTTCCTTAATGGTGCTTG
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=301,22
PRIMER_LEFT_1_TM=57.957
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=299
PRIMER_PAIR_PENALTY_2=2.1802
PRIMER_LEFT_2_PENALTY=2.042650
PRIMER_RIGHT_2_PENALTY=0.137575
PRIMER_LEFT_2_SEQUENCE=CATGAGTGATGTNGGTCTGAGT
PRIMER_RIGHT_2_SEQUENCE=AGTGGTGGATGAGAGGTTTGAT
PRIMER_LEFT_2=4,22
PRIMER_RIGHT_2=201,22
PRIMER_LEFT_2_TM=57.957
PRIMER_RIGHT_2_TM=59.862
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=198
PRIMER_PAIR_PENALTY_3=2.1802
PRIMER_LEFT_3_PENALTY=2.042650
PRIMER_RIGHT_3_PENALTY=0.137575
PRIMER_LEFT_3_SEQUENCE=ACATGAGTGATGTNGGTCTGAG
PRIMER_RIGHT_3_SEQUENCE=AGTGGTGGATGAGAGGTTTGAT
PRIMER_LEFT_3=3,22
PRIMER_RIGHT_3=201,22
PRIMER_LEFT_3_TM=57.957
PRIMER_RIGHT_3_TM=59.862
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=199
PRIMER_PAIR_PENALTY_4=2.1983
PRIMER_LEFT_4_PENALTY=2.111285
PRIMER_RIGHT_4_PENALTY=0.087031
PRIMER_LEFT_4_SEQUENCE=ACATGAGTGATGTNGGTCTGAGT
PRIMER_RIGHT_4_SEQUENCE=AGCTCTTCCTTAATGGTGCTTG
PRIMER_LEFT_4=3,23
PRIMER_RIGHT_4=301,22
PRIMER_LEFT_4_TM=58.889
PRIMER_RIGHT_4_TM=59.913
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=299
=
PRIMER_SEQUENCE_ID=SSR_06133 Ps_contig_mira-and-tgicl-ass_franssen_41871
SEQUENCE=ATTACATGAGTGATGTNGGTCTGAGTAAACCAAACCAAACCAAACCTTTAAACTCATCACATGGTTATGGCCTTATCTTCTTCTTCTTCTCTCATTCTCAACACCACTGATTATTCACCTCACAAACCACTCCATTCACCATCATCCTCCTCATCATCATTTTCTTCTTCTCACTTTCCAATCAAACCTCTCATCCACCACTCTCACAACCTTCCCATATCTCAAAAATGGAGAAGAAATGTTTCATTCTTTACTTCTTTCTTAAAGAAACCCAAAGACGCAAGCACCATTAAGGAAGAGCTTCTTGAAGCCATTGCTTCGCTTGATCGAGGAGCTGATGCCACTCCTGAAGACCAACAAAGCGTTGATCAGATTGCACGCAAACTTGAAGCAGTTAATCCAACAAAGGCGCCACTCAAATCTAGTTTACTTGATGGAAAATGGGAGCTTATTTACACTACCTCTCAGTCAATCTTGCAAACTAAGAGACCAAAAATTTTGAGATCAGTTACAAATTATCAAGCAATCAATGCTGATACTCTCCGAGCCCAAAACATGGAATCTCTGCCATTCTTCAACCAGGTGACAGCAGATTTAACACCTTTGAACACAAGGAAAGTGGCTGTGAAATTCGATACCTTCAAAATTGCAGGCTTCATACCTGTTAAGGCTCCTGACACAGCTCGCGGCGAACTTGAAATAACATATTTGGATGAAGAACTTCGGGTATCAAGAGGGGACAAAGGAAACTTGTTCATCTTGAAAATGGTGGATCCATCTTATCGGATTCCTGCATGATGTGTCGTCACTGTAGCATTCTATCGCTATATATCGTGTTTTTTCCCTCTCAACGGATTCAAACTACTATCAGAGCTTTCTGAAGTTGGAACTTATACTAGCAAGCCAATGCATACCACTACTCAAGCTTAACTTGAAGCATTTTGGAACTGTGCTTTCTGTACAGAAGTGGTTGTAAAATCTTTACATACTGAGAGTTTTAGTGCAAATTGCTTCTTTCAAATATCAGCGGAATTTGATAGTTCTTCATTATGTATTATTTTTAGAGTTCTTTTGAAGTAGTTTTCAACTTAAATAAGTAATATTTTAATAAGTTTTATAGTTAAAAGTAAAGAAAGTAAAAGTCTTGAAACTCTTGAGTTATTTTGTTTGTCTTATTGTTAATTCTTCTTAACAAGAAGTCATTTGTGTAATGCATAAGCTTATTGCCTTTGTAGGTT
TARGET=76,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5476
PRIMER_LEFT_PENALTY=0.460533
PRIMER_RIGHT_PENALTY=0.087031
PRIMER_LEFT_SEQUENCE=GTAAACCAAACCAAACCAAACC
PRIMER_RIGHT_SEQUENCE=AGCTCTTCCTTAATGGTGCTTG
PRIMER_LEFT=24,22
PRIMER_RIGHT=301,22
PRIMER_LEFT_TM=59.539
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=278
PRIMER_PAIR_PENALTY_1=0.6193
PRIMER_LEFT_1_PENALTY=0.460533
PRIMER_RIGHT_1_PENALTY=0.158723
PRIMER_LEFT_1_SEQUENCE=GTAAACCAAACCAAACCAAACC
PRIMER_RIGHT_1_SEQUENCE=GTTGTGAGAGTGGTGGATGAGA
PRIMER_LEFT_1=24,22
PRIMER_RIGHT_1=209,22
PRIMER_LEFT_1_TM=59.539
PRIMER_RIGHT_1_TM=60.159
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=186
PRIMER_PAIR_PENALTY_2=0.6226
PRIMER_LEFT_2_PENALTY=0.460533
PRIMER_RIGHT_2_PENALTY=0.162074
PRIMER_LEFT_2_SEQUENCE=GTAAACCAAACCAAACCAAACC
PRIMER_RIGHT_2_SEQUENCE=GATGAGGAGGATGATGGTGAAT
PRIMER_LEFT_2=24,22
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=59.539
PRIMER_RIGHT_2_TM=60.162
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=131
PRIMER_PAIR_PENALTY_3=0.7262
PRIMER_LEFT_3_PENALTY=0.639172
PRIMER_RIGHT_3_PENALTY=0.087031
PRIMER_LEFT_3_SEQUENCE=ACTCATCACATGGTTATGGCCT
PRIMER_RIGHT_3_SEQUENCE=AGCTCTTCCTTAATGGTGCTTG
PRIMER_LEFT_3=51,22
PRIMER_RIGHT_3=301,22
PRIMER_LEFT_3_TM=60.639
PRIMER_RIGHT_3_TM=59.913
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=251
PRIMER_PAIR_PENALTY_4=0.7586
PRIMER_LEFT_4_PENALTY=0.460533
PRIMER_RIGHT_4_PENALTY=0.298018
PRIMER_LEFT_4_SEQUENCE=GTAAACCAAACCAAACCAAACC
PRIMER_RIGHT_4_SEQUENCE=TTGTGAGAGTGGTGGATGAGAG
PRIMER_LEFT_4=24,22
PRIMER_RIGHT_4=208,22
PRIMER_LEFT_4_TM=59.539
PRIMER_RIGHT_4_TM=60.298
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=185
=
PRIMER_SEQUENCE_ID=SSR_06134 Ps_contig_mira-and-tgicl-ass_franssen_41877
SEQUENCE=GGCACGAGGATGCTTTGAGCGGTTCCTTGAAAATGTCAAAAATAAACTTTTCTATAAATAACTCTACCACTAGTTTTTTCGCTACACTTCATCATCAATATCATCAACAAAACCTAAGTTAATTCTGTTCCACACAAGCTTTTCAAATCATACATTCATATTCATCGCAAAAATGTTGAATGAAGGGAATGAAAATGTTTTAAAGGGAGGAAGTGGAAGCARRNNAGGGAAGATGGTGTCTCCAAACAACAAAGAATTTATGTTCATATGTTTTCTTGTGTCTCTTTGGTACTCATCAAACATTGGTGTCATTCTTCTCAACAAATACCTTCTCTCAAATTATGGTTTCAAGTTTCCAATCTTCCTTACAATGTGTCACATGTCAGCTTGTGCTATTTTTAGCTACATCTCCATTGTGTTCTTCAAGGTTGTTCCTCAACAGATGATTAAATCAAGGTCACAGTTCTTGAAGATTGCAACTTTGAGCATTGTTTTTTGTGCGTCGGTGGTTGGTGGCAACATTTCTCTGAGGTATTTGGCTGTTTCTTTTAACCAAGCGGTGGGTGCAACCACACCTTTCTTCACGGCTGTTTTTGCTTATTTGGCTACCTTTAAGAGAGAAGCTTGGATTACATATGCTGCTCTTGTTCCTGTTGTTGCTGGTGTTGTCATTGCAAGTGGGGGCGAGCCAGGGTTTCACGTATTCGGATTTGTTATGTGCCTAAGCGCAACCGCTGCAAGAGCTTTCAAATCTGTTCTTCAGGGCATTTTACTTTCTTCTGAAGGGGAGAAGTTGAACTCGATGAATTTGCTCCTGTATATGTCTCCGATCGCAGTCGTACTTTTGTTGCCTGCAGCGCTTATCATGGAGCCAAATGTTATAGATGTCACCTTAACACTTGGAAAGGAACATAAATTTATGGGTGTGCTTCTTCTTCTTAACTCCGCCACAGCATATGCAGCAAACTTAACAAACTTCCTGGTGACAAAACATACAAGTGCTTTGACACTCCAGGTATTAGGCAATGCAAAAGGCGCTGTAGCTGTTGTTATCTCAATACTGATATTCAGAAACCCTGTCACTGTTATTGGCATGGGTGGCTATGCAGTTACTGTAATGGGAGTTGTTGCATATGGAGAAACAAAAAGGAGGTTTAGATGAACAGTTCCCTCCCATTCACTGATTGTCATATTCATGTATTTTATTGTAGTTTATTTACATCATTAAGATTATTTGTAACGTGACTAGGAGTAGGGGCGACGACGCGGGGGACGTGACGTGATTTTATAATGTTATTCTGTTGTATCTAGCACATAAATATTTATATATCATTAGTACATTGTTATGATCTAATTGAAGGATCACTTGTTACGTACATAAAA
TARGET=929,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06135 Ps_contig_mira-and-tgicl-ass_franssen_41882
SEQUENCE=GGATTGATGACAGATTTGAATTTGACACAGAAGAATGCAACATTGCTTAATGGAAAATGGGTTTTTAACCAATCAATAAAGCCTCTCTATACTGACATAACTTGTCCATATATAGATAGACAATTTTCTTGTGTAAAAAATGGTAGAAAGGATTTTGATTATCAACATTGGGAGTGGCAGCCRGAAGATTGTACCTTGCCAAGTTTCAATGCAGCGTTGGCCCTTAAAAAGCTTCAAGGGAAAAGACTACTATTTGTAGGGGACTCACTACAAAGAAATCAATGGGAATCTTTTGTTTGTTTGGTAGAATGGATCATACCAGAAAAGATGAAATCTATAGAAAAGAGGGATTGTTCATTCAGTCTTCAAAGCCAAGGAATACAATGCTACTGTAGAATTCTATTGGCTCCATATCTTGGTTAGACGTACCAACACTGAACAATAAACAT
TARGET=292,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06136 Ps_contig_mira-and-tgicl-ass_franssen_41915
SEQUENCE=TTACCCTTTCTTCATCTTCTTCTTCTGGTCTATAATCTTCTTGTTTATCAGGTTCAGAGTCAGAGGCACTATCTTCATCCGAGTCGGGCAACACAACGACGGGCGCTGAACGTTGTCTGACGGCTGAGATTTTGTCGTTTACTGATGTTGATGAGTGAAGACCTTCATTTAAAACACTGCTGGGTTCCAAGTGTAGAAGTAAGGAAAACAGTCGGCATAAGAACTCATCCAACCATTCAGAAAAACGGATCATTGGCATAAAGGATAAGTCATCAATTTTATCATCCAATAAAGCCAAATTTGAAAATATGGAACCAATTAATTGCATGGTAGCTTAAAGTTTTCGGAGGGTCGTTAGCATCCATGCCAAGTAATGCATTGGAATAATAACCG
TARGET=15,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06137 Ps_contig_mira-and-tgicl-ass_franssen_41918
SEQUENCE=AAACTCACAGAGAARRAAAATACCAAARGTTAAATTTAAGTACAAGAAAGCTGATGAAAGAAACCAAGACAAGATGGCCATTGCAGCAGATATTTGGTATCTACTACATAAATTTTCTGGACAATACGATTTATCAGATTTCATCAACAAATCTGTCACACTAGCAGTTGAACATGCTGCAGCTAATAACAGATATGACAAAACCATGTCGCCCACAGTAATCGTAAAAATGAATCTTGGTTGCTTCGGTACTTTCTTTATGAAGATCGAATACCCGTCTACCACCAATAGTGTTGTGCTCCAGGATATTACCAAACCCATCACCATTACCAAAAAGCAGAAAGCAGAATAGCCATAGAAATCAACATCCAAACACATATAGAAAAGGGAAGCAGAGGAAAAGACGGTTTGGCCCAATCGAAGAGCAAAACTGGAACTGGTTCCTAACGCCCCTGGCAATTCCTCCATTCCTTCTCATCATGATAATTCAATCAGAAACATACTCATCATACATAGATAAACAAACATCAATGAAGAAAACAAGAACTAAATTACTAATGTTTTTGATTAGGAAAGTGAGGGAGTTTAGTTAGTTCTTGTGAGTATTAATTAATTAAAGGAAAGTAGGTATGAAGTATTCTGGTCCCTTCATTATTTTTGTAGTTAGAAATAGGATGAGTTATTGTCGGCGCTGTACATGAAACATGG
TARGET=605,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06138 Ps_contig_mira-and-tgicl-ass_franssen_41927
SEQUENCE=AAAGAATTTATCTACAACACAAAGGGAAATGCAATAATAATAATAATATACATAAATGAGACAAGTAACATTCAAATTTTCTGCATACAATGAATGGTGATTCCAGAAACTAAACTGAAAACTCATATGTGCAGCATCGTCTTTATACATTTTCAGTCTAACACCATTCATTCAAACATATGTCTCAGATTGTAATTTGTAACATAATCTGTTTAAACATGTTATTCACAAGCAATCTTGGTATCGAAGCTGCTGAGGCAGATTGCGAATGCCTGAAATGCCGAGATAGGGTATCGGTAATCCATTGTAAATAAATCCTTCCCAACTTTTCCGAATTGCAGAATAATCTTATCGTGTTCGGGTCCAGCAGTTCCGTTCTCTGCAGAAGCCACCAGCTGAAAGTTTTTAACCGAGGCAACTGTCACCCGTCCGTGAAAGTTTAAGCACCAACACTGCAGCTGCTCGTGCCACCTCGGAGCCTTGTTTTTCAACACAAGTAAACCGTCTTTTTGATCACCGACTAGGGGTCCGGAGAGAGAATTCTCCATCACACGATTTGATTTTGATCGGAAAAAAGGAAACATGGGAAACATTTCTATGTTGTTTAGAGAAAACTCGGTTTGTGTAGGAGCTACTCCTCCTGGTTCGATCGAAGAGGATGGTATGGAATCCATGACACAGTGCATTCTCCTAGGCCCCCTCGAGCCAAGGACGTTCAATTCATATGAGATGTGAGCCACGGGATAGTTGCCGGTTGGGACCTTGGGTGAAACCTGCTTTAGATTCACCAACCTAGTAGAACGACTTTTAGTAAACTTAGCTCCTGTATGAGGTGGCTGGCTGTCATAGATTGTGAACTTTGTTCCGAGGAAATTTGATCTCAGTTTCCCAACATAGGTATTGCTTCCCCTTGACATATCATCGGCGTCTAGAGAGATGATATAATCCGTGCAGGTAGGACGTCTACTTTTGCGAGCAGCCAGAAGGAACTTCCCGTCATCACCTAACGAACTAGTTAAACTGAGAAACAAATAATATGTTTGAGTGGACCGGTTGCGCTTTATAAAGCATTGAAGGAGATTCTCCCTTGGACCGGGCTGTTTAACAGAAATGGGGAAAGTTATCTTAGCAGAAAGTTCAGGTTTTTTAACGATTTCTTTGGTGATGACTCTCCAATTCCGACAAACTCCGGCACATGCGACAACGTTCTTCCTCGGCGGCCACGTGTCCTCGGACGCTTCAATTCGAAGGAGAACTTCACGGAGAAGCTCTTGTGGCATGTTGGCCCAACAACTCTGTCTTAAACCGTCGCCAACCACCACTTCCTTCTCCACTACAACAACTACCCTTTGAGATCTCGATCTCATGTCTTCTATTATGTTCCTGATTATAGCCATACCCAGATCACCAAATGGTGAAAAAATTGAACTTTGAAAAGAGAATTATGAGAGAGATGCGTTGATTGAGGGTTGAAAAGGGAAAGGTGAGATTGTGATGATAAAAGGAGAATTGAGTTTGATTATTTTTTGTGTGAGAGGAAAGGAAATGTGCGAAAAGAGTGTAAGAGAGAGAGATAAGAAGAAGAAGAAGAAGAAGRAGAAGTCAGAGT
TARGET=33,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06139 Ps_contig_mira-and-tgicl-ass_franssen_41927
SEQUENCE=AAAGAATTTATCTACAACACAAAGGGAAATGCAATAATAATAATAATATACATAAATGAGACAAGTAACATTCAAATTTTCTGCATACAATGAATGGTGATTCCAGAAACTAAACTGAAAACTCATATGTGCAGCATCGTCTTTATACATTTTCAGTCTAACACCATTCATTCAAACATATGTCTCAGATTGTAATTTGTAACATAATCTGTTTAAACATGTTATTCACAAGCAATCTTGGTATCGAAGCTGCTGAGGCAGATTGCGAATGCCTGAAATGCCGAGATAGGGTATCGGTAATCCATTGTAAATAAATCCTTCCCAACTTTTCCGAATTGCAGAATAATCTTATCGTGTTCGGGTCCAGCAGTTCCGTTCTCTGCAGAAGCCACCAGCTGAAAGTTTTTAACCGAGGCAACTGTCACCCGTCCGTGAAAGTTTAAGCACCAACACTGCAGCTGCTCGTGCCACCTCGGAGCCTTGTTTTTCAACACAAGTAAACCGTCTTTTTGATCACCGACTAGGGGTCCGGAGAGAGAATTCTCCATCACACGATTTGATTTTGATCGGAAAAAAGGAAACATGGGAAACATTTCTATGTTGTTTAGAGAAAACTCGGTTTGTGTAGGAGCTACTCCTCCTGGTTCGATCGAAGAGGATGGTATGGAATCCATGACACAGTGCATTCTCCTAGGCCCCCTCGAGCCAAGGACGTTCAATTCATATGAGATGTGAGCCACGGGATAGTTGCCGGTTGGGACCTTGGGTGAAACCTGCTTTAGATTCACCAACCTAGTAGAACGACTTTTAGTAAACTTAGCTCCTGTATGAGGTGGCTGGCTGTCATAGATTGTGAACTTTGTTCCGAGGAAATTTGATCTCAGTTTCCCAACATAGGTATTGCTTCCCCTTGACATATCATCGGCGTCTAGAGAGATGATATAATCCGTGCAGGTAGGACGTCTACTTTTGCGAGCAGCCAGAAGGAACTTCCCGTCATCACCTAACGAACTAGTTAAACTGAGAAACAAATAATATGTTTGAGTGGACCGGTTGCGCTTTATAAAGCATTGAAGGAGATTCTCCCTTGGACCGGGCTGTTTAACAGAAATGGGGAAAGTTATCTTAGCAGAAAGTTCAGGTTTTTTAACGATTTCTTTGGTGATGACTCTCCAATTCCGACAAACTCCGGCACATGCGACAACGTTCTTCCTCGGCGGCCACGTGTCCTCGGACGCTTCAATTCGAAGGAGAACTTCACGGAGAAGCTCTTGTGGCATGTTGGCCCAACAACTCTGTCTTAAACCGTCGCCAACCACCACTTCCTTCTCCACTACAACAACTACCCTTTGAGATCTCGATCTCATGTCTTCTATTATGTTCCTGATTATAGCCATACCCAGATCACCAAATGGTGAAAAAATTGAACTTTGAAAAGAGAATTATGAGAGAGATGCGTTGATTGAGGGTTGAAAAGGGAAAGGTGAGATTGTGATGATAAAAGGAGAATTGAGTTTGATTATTTTTTGTGTGAGAGGAAAGGAAATGTGCGAAAAGAGTGTAAGAGAGAGAGATAAGAAGAAGAAGAAGAAGAAGRAGAAGTCAGAGT
TARGET=1564,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06140 Ps_contig_mira-and-tgicl-ass_franssen_41927
SEQUENCE=AAAGAATTTATCTACAACACAAAGGGAAATGCAATAATAATAATAATATACATAAATGAGACAAGTAACATTCAAATTTTCTGCATACAATGAATGGTGATTCCAGAAACTAAACTGAAAACTCATATGTGCAGCATCGTCTTTATACATTTTCAGTCTAACACCATTCATTCAAACATATGTCTCAGATTGTAATTTGTAACATAATCTGTTTAAACATGTTATTCACAAGCAATCTTGGTATCGAAGCTGCTGAGGCAGATTGCGAATGCCTGAAATGCCGAGATAGGGTATCGGTAATCCATTGTAAATAAATCCTTCCCAACTTTTCCGAATTGCAGAATAATCTTATCGTGTTCGGGTCCAGCAGTTCCGTTCTCTGCAGAAGCCACCAGCTGAAAGTTTTTAACCGAGGCAACTGTCACCCGTCCGTGAAAGTTTAAGCACCAACACTGCAGCTGCTCGTGCCACCTCGGAGCCTTGTTTTTCAACACAAGTAAACCGTCTTTTTGATCACCGACTAGGGGTCCGGAGAGAGAATTCTCCATCACACGATTTGATTTTGATCGGAAAAAAGGAAACATGGGAAACATTTCTATGTTGTTTAGAGAAAACTCGGTTTGTGTAGGAGCTACTCCTCCTGGTTCGATCGAAGAGGATGGTATGGAATCCATGACACAGTGCATTCTCCTAGGCCCCCTCGAGCCAAGGACGTTCAATTCATATGAGATGTGAGCCACGGGATAGTTGCCGGTTGGGACCTTGGGTGAAACCTGCTTTAGATTCACCAACCTAGTAGAACGACTTTTAGTAAACTTAGCTCCTGTATGAGGTGGCTGGCTGTCATAGATTGTGAACTTTGTTCCGAGGAAATTTGATCTCAGTTTCCCAACATAGGTATTGCTTCCCCTTGACATATCATCGGCGTCTAGAGAGATGATATAATCCGTGCAGGTAGGACGTCTACTTTTGCGAGCAGCCAGAAGGAACTTCCCGTCATCACCTAACGAACTAGTTAAACTGAGAAACAAATAATATGTTTGAGTGGACCGGTTGCGCTTTATAAAGCATTGAAGGAGATTCTCCCTTGGACCGGGCTGTTTAACAGAAATGGGGAAAGTTATCTTAGCAGAAAGTTCAGGTTTTTTAACGATTTCTTTGGTGATGACTCTCCAATTCCGACAAACTCCGGCACATGCGACAACGTTCTTCCTCGGCGGCCACGTGTCCTCGGACGCTTCAATTCGAAGGAGAACTTCACGGAGAAGCTCTTGTGGCATGTTGGCCCAACAACTCTGTCTTAAACCGTCGCCAACCACCACTTCCTTCTCCACTACAACAACTACCCTTTGAGATCTCGATCTCATGTCTTCTATTATGTTCCTGATTATAGCCATACCCAGATCACCAAATGGTGAAAAAATTGAACTTTGAAAAGAGAATTATGAGAGAGATGCGTTGATTGAGGGTTGAAAAGGGAAAGGTGAGATTGTGATGATAAAAGGAGAATTGAGTTTGATTATTTTTTGTGTGAGAGGAAAGGAAATGTGCGAAAAGAGTGTAAGAGAGAGAGATAAGAAGAAGAAGAAGAAGAAGRAGAAGTCAGAGT
TARGET=1576,21
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06141 Ps_contig_mira-and-tgicl-ass_franssen_41928
SEQUENCE=CGATTAGTGGCCCTACCGGTGATCAGAGCCCTTAGGAGCAGCAGCAGCCTTTTGGACAGCCTCATTTGTCACCCTTGGGGAGGACTTCCTTGAAGCCAAAGCGGAAGCATTAGAATAAAAGTAAGCCTCATCCTTTTGTCCAGACAATGTCTTGGCCAAGGCATTTACTTCAACTATTTTCTGTGCTGCAAACGCAAGCCAAGACTTAATCTCGTGGTCCAGCTTCGCCTCATTCACCAGATCAACTGCAGTGTGAAGAAGAGAACAGGATGTGGAAACCACAACTT
TARGET=37,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.7127
PRIMER_LEFT_PENALTY=2.683409
PRIMER_RIGHT_PENALTY=0.029253
PRIMER_LEFT_SEQUENCE=TACCGGTGATCAGAGCCCTTAG
PRIMER_RIGHT_SEQUENCE=CATTGTCTGGACAAAAGGATGA
PRIMER_LEFT=13,22
PRIMER_RIGHT=148,22
PRIMER_LEFT_TM=62.683
PRIMER_RIGHT_TM=59.971
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=2.7643
PRIMER_LEFT_1_PENALTY=2.683409
PRIMER_RIGHT_1_PENALTY=0.080887
PRIMER_LEFT_1_SEQUENCE=TACCGGTGATCAGAGCCCTTAG
PRIMER_RIGHT_1_SEQUENCE=GAGATTAAGTCTTGGCTTGCGT
PRIMER_LEFT_1=13,22
PRIMER_RIGHT_1=212,22
PRIMER_LEFT_1_TM=62.683
PRIMER_RIGHT_1_TM=59.919
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=200
PRIMER_PAIR_PENALTY_2=2.7643
PRIMER_LEFT_2_PENALTY=2.683409
PRIMER_RIGHT_2_PENALTY=0.080887
PRIMER_LEFT_2_SEQUENCE=TACCGGTGATCAGAGCCCTTAG
PRIMER_RIGHT_2_SEQUENCE=ACGAGATTAAGTCTTGGCTTGC
PRIMER_LEFT_2=13,22
PRIMER_RIGHT_2=214,22
PRIMER_LEFT_2_TM=62.683
PRIMER_RIGHT_2_TM=59.919
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=2.7907
PRIMER_LEFT_3_PENALTY=2.761472
PRIMER_RIGHT_3_PENALTY=0.029253
PRIMER_LEFT_3_SEQUENCE=GGTGATCAGAGCCCTTAGGA
PRIMER_RIGHT_3_SEQUENCE=CATTGTCTGGACAAAAGGATGA
PRIMER_LEFT_3=17,20
PRIMER_RIGHT_3=148,22
PRIMER_LEFT_3_TM=59.239
PRIMER_RIGHT_3_TM=59.971
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=132
PRIMER_PAIR_PENALTY_4=2.8370
PRIMER_LEFT_4_PENALTY=2.807765
PRIMER_RIGHT_4_PENALTY=0.029253
PRIMER_LEFT_4_SEQUENCE=ATTAGTGGCCCTACCGGTGAT
PRIMER_RIGHT_4_SEQUENCE=CATTGTCTGGACAAAAGGATGA
PRIMER_LEFT_4=2,21
PRIMER_RIGHT_4=148,22
PRIMER_LEFT_4_TM=61.808
PRIMER_RIGHT_4_TM=59.971
PRIMER_LEFT_4_GC_PERCENT=52.381
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=147
=
PRIMER_SEQUENCE_ID=SSR_06142 Ps_contig_mira-and-tgicl-ass_franssen_41931
SEQUENCE=CAATAGAAGGTTTTCTCCAAAAAGGAAAAGAAAAAGATAAAGAAAAAGACTAGGGGGAGTTAGAATGGGAAATCATTCATCAGAAAAATGAGAGTTCAACCAGAGAGAATCAGCAGCACCAACACAATGTCACAACCAAGAAGACCACTCCACATCAACATCCCCCCATCATCATCATCATCCGCCATGTTCCAACGGATCCGCCCTTCCCTCGTCACCTCATTTCTCCAAAACCACTTTCTCTCCTTTCGTTCTCTCCATTTTCCTCTTCCTCGCTTGGATCTCTCTCCGTTCATATCGCACTTCTCATTCTCTTCACCTCTCTAACCCAAACGACGACGTTAAAGCCAATCTCCGTAGATTCTCCTCTCATTTCCCTTCCCCTATTACGAAGGATAATCGGGGTTGGATTCTCGATCCTATCGCTGTTGCAGCTTCTTCCAGCCTTTCAGGTACTTCCTTCCTCTTTGTTCTCTTAATTTAATNAATTCGACCGAATTGAGGAATCATAGACACCA
TARGET=167,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1376
PRIMER_LEFT_PENALTY=0.099438
PRIMER_RIGHT_PENALTY=0.038125
PRIMER_LEFT_SEQUENCE=AACCAAGAAGACCACTCCACAT
PRIMER_RIGHT_SEQUENCE=TTCGTAATAGGGGAAGGGAAAT
PRIMER_LEFT=133,22
PRIMER_RIGHT=392,22
PRIMER_LEFT_TM=59.901
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=260
PRIMER_PAIR_PENALTY_1=0.1571
PRIMER_LEFT_1_PENALTY=0.099438
PRIMER_RIGHT_1_PENALTY=0.057688
PRIMER_LEFT_1_SEQUENCE=AACCAAGAAGACCACTCCACAT
PRIMER_RIGHT_1_SEQUENCE=AACCCCGATTATCCTTCGTAAT
PRIMER_LEFT_1=133,22
PRIMER_RIGHT_1=406,22
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=59.942
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=274
PRIMER_PAIR_PENALTY_2=0.1596
PRIMER_LEFT_2_PENALTY=0.099438
PRIMER_RIGHT_2_PENALTY=0.060196
PRIMER_LEFT_2_SEQUENCE=AACCAAGAAGACCACTCCACAT
PRIMER_RIGHT_2_SEQUENCE=CGATATGAACGGAGAGAGATCC
PRIMER_LEFT_2=133,22
PRIMER_RIGHT_2=299,22
PRIMER_LEFT_2_TM=59.901
PRIMER_RIGHT_2_TM=60.060
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=167
PRIMER_PAIR_PENALTY_3=0.1866
PRIMER_LEFT_3_PENALTY=0.148509
PRIMER_RIGHT_3_PENALTY=0.038125
PRIMER_LEFT_3_SEQUENCE=CAAGAAGACCACTCCACATCAA
PRIMER_RIGHT_3_SEQUENCE=TTCGTAATAGGGGAAGGGAAAT
PRIMER_LEFT_3=136,22
PRIMER_RIGHT_3=392,22
PRIMER_LEFT_3_TM=60.149
PRIMER_RIGHT_3_TM=60.038
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=0.1876
PRIMER_LEFT_4_PENALTY=0.099438
PRIMER_RIGHT_4_PENALTY=0.088198
PRIMER_LEFT_4_SEQUENCE=AACCAAGAAGACCACTCCACAT
PRIMER_RIGHT_4_SEQUENCE=GGAAGGGAAATGAGAGGAGAAT
PRIMER_LEFT_4=133,22
PRIMER_RIGHT_4=381,22
PRIMER_LEFT_4_TM=59.901
PRIMER_RIGHT_4_TM=59.912
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=249
=
PRIMER_SEQUENCE_ID=SSR_06143 Ps_contig_mira-and-tgicl-ass_franssen_41932
SEQUENCE=TCCCCTAATATTTATTATTACACTTAATTTCCATTGGATTTCAATTCAACTATGGACGCCGAAGACGACGAAGCTCAACCTAACCAATCTCCTCCACATGCATCCTCCGACTCGCCGCCGCAGCCGCCGAACCTCTCAACCAATGCCGACGACATCCCCAAAGACGGAGACCTTGAAACCGTGGTTGCAGGTGAGGAGGAGGAGGCCGGCGATGCCACCGCAAACACGGATGTTGCAGTTGCTGACGTGTCATCAAC
TARGET=193,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.1387
PRIMER_LEFT_PENALTY=0.050443
PRIMER_RIGHT_PENALTY=2.088261
PRIMER_LEFT_SEQUENCE=AGACGACGAAGCTCAACCTAAC
PRIMER_RIGHT_SEQUENCE=ACGTCAGCAACTGCAACATC
PRIMER_LEFT=62,22
PRIMER_RIGHT=247,20
PRIMER_LEFT_TM=59.950
PRIMER_RIGHT_TM=59.912
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.7000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=186
PRIMER_PAIR_PENALTY_1=2.2243
PRIMER_LEFT_1_PENALTY=0.136017
PRIMER_RIGHT_1_PENALTY=2.088261
PRIMER_LEFT_1_SEQUENCE=AACCTAACCAATCTCCTCCACA
PRIMER_RIGHT_1_SEQUENCE=ACGTCAGCAACTGCAACATC
PRIMER_LEFT_1=76,22
PRIMER_RIGHT_1=247,20
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=59.912
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=172
PRIMER_PAIR_PENALTY_2=2.2243
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=2.088261
PRIMER_LEFT_2_SEQUENCE=CAACCTAACCAATCTCCTCCAC
PRIMER_RIGHT_2_SEQUENCE=ACGTCAGCAACTGCAACATC
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=247,20
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.912
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=6.3000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=173
PRIMER_PAIR_PENALTY_3=2.3048
PRIMER_LEFT_3_PENALTY=0.216588
PRIMER_RIGHT_3_PENALTY=2.088261
PRIMER_LEFT_3_SEQUENCE=CGAAGCTCAACCTAACCAATCT
PRIMER_RIGHT_3_SEQUENCE=ACGTCAGCAACTGCAACATC
PRIMER_LEFT_3=68,22
PRIMER_RIGHT_3=247,20
PRIMER_LEFT_3_TM=59.783
PRIMER_RIGHT_3_TM=59.912
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=180
PRIMER_PAIR_PENALTY_4=2.4301
PRIMER_LEFT_4_PENALTY=0.341875
PRIMER_RIGHT_4_PENALTY=2.088261
PRIMER_LEFT_4_SEQUENCE=ACGAAGCTCAACCTAACCAATC
PRIMER_RIGHT_4_SEQUENCE=ACGTCAGCAACTGCAACATC
PRIMER_LEFT_4=67,22
PRIMER_RIGHT_4=247,20
PRIMER_LEFT_4_TM=59.658
PRIMER_RIGHT_4_TM=59.912
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_06144 Ps_contig_mira-and-tgicl-ass_franssen_41943
SEQUENCE=GCGTAAATCGGAGGGTGGGCTGCCACGTCACAATATCTTATATCCTGCACTCCAAAATCCTATAAATACACCACATACCAATTTCTACACAAATTNATACCAACACATTTCTCAACCTCTTCATTACTTTCTTCAAACCCAAAAATCAATCAATCACTACAGATATGGAAGACTCCATTCAAATCAAGGTTGAGGATAAAACACCGGTTGATGTAAAGGAAGAGAAGGTTGAGATAGAAAAGGATTTGGAGAGTACAAAATGTAAGGGAGAGAAGAAACATAATGATGAAGGTGGGGATGATGAGAAGAAAAAGAAAAAGAAGGATAAAACTGATGCAGAGGGGAAGGAGAAGAAGAAAAAGGGAAAAGATGAGAAAACTGATGTAGAGAAGGTTAAGGATAAAGAAGATGGTGATGGAAATAAGGATAAGGAGAAAAAGAAAAAGGAAAAGAAGGGAAAAGAAGATGGAGACAAGGATGGTGAAGAAAAGAAGGAGAAGAATGAAGAGGGTGAGGAGGGTGATGGAGATAAGAAAAAGAAGAATAAGGACAAGAAAGAGAAAAAGAAGGATAAAAATGAGAAAGAAGAGGGTGAAGTATCTATAAGGGATATTGATATAGAAGAAGATGAACTANNAAGTTAAAGAGAGGA
TARGET=144,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2141
PRIMER_LEFT_PENALTY=0.159203
PRIMER_RIGHT_PENALTY=0.054900
PRIMER_LEFT_SEQUENCE=GCTGCCACGTCACAATATCTTA
PRIMER_RIGHT_SEQUENCE=CCCCACCTTCATCATTATGTTT
PRIMER_LEFT=18,22
PRIMER_RIGHT=296,22
PRIMER_LEFT_TM=60.159
PRIMER_RIGHT_TM=59.945
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.4583
PRIMER_LEFT_1_PENALTY=0.159203
PRIMER_RIGHT_1_PENALTY=0.299089
PRIMER_LEFT_1_SEQUENCE=GCTGCCACGTCACAATATCTTA
PRIMER_RIGHT_1_SEQUENCE=TTTTTCTTCTCATCATCCCCAC
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=312,22
PRIMER_LEFT_1_TM=60.159
PRIMER_RIGHT_1_TM=60.299
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=295
PRIMER_PAIR_PENALTY_2=0.5226
PRIMER_LEFT_2_PENALTY=0.159203
PRIMER_RIGHT_2_PENALTY=0.363436
PRIMER_LEFT_2_SEQUENCE=GCTGCCACGTCACAATATCTTA
PRIMER_RIGHT_2_SEQUENCE=ACATCAACCGGTGTTTTATCCT
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=213,22
PRIMER_LEFT_2_TM=60.159
PRIMER_RIGHT_2_TM=59.637
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=196
PRIMER_PAIR_PENALTY_3=0.5579
PRIMER_LEFT_3_PENALTY=0.159203
PRIMER_RIGHT_3_PENALTY=0.398742
PRIMER_LEFT_3_SEQUENCE=GCTGCCACGTCACAATATCTTA
PRIMER_RIGHT_3_SEQUENCE=ATCATCCCCACCTTCATCATTA
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=301,22
PRIMER_LEFT_3_TM=60.159
PRIMER_RIGHT_3_TM=60.399
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=284
PRIMER_PAIR_PENALTY_4=0.5821
PRIMER_LEFT_4_PENALTY=0.159203
PRIMER_RIGHT_4_PENALTY=0.422888
PRIMER_LEFT_4_SEQUENCE=GCTGCCACGTCACAATATCTTA
PRIMER_RIGHT_4_SEQUENCE=CTTTTTCTTCTCATCATCCCCA
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=313,22
PRIMER_LEFT_4_TM=60.159
PRIMER_RIGHT_4_TM=60.423
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=11.2000
PRIMER_PAIR_4_COMPL_ANY=2.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=296
=
PRIMER_SEQUENCE_ID=SSR_06145 Ps_contig_mira-and-tgicl-ass_franssen_41950
SEQUENCE=CCGGGACCCGTACCAAAACCAAGGGAAAGGGACGACAAACAACCGCAAGCAATGAACGGATTGCCATCAACAGTACTACCAGTATTATCACCACATATCTGGCAGACTTGGCTACCCATCACCTTCATCGGCGTTATCCGGTATTGACATGTCACTGTCGGTGTCGTGTTTCCTGTGTTCGTGCTTCATAGATCTCATCCCAGATTCCCCTTCCGAGTCCATCATGGTCTCCTACTTAGCTCTTCCTAGATCCCAATAAGGAATCAAATAGTAATCCAAACTCAACAAGTTACACACATCACTTCAAACACTTTATCACTTCCCAATATTCCTAATCCACTTCAGAAGCAACTCCTTCTTTTTTATTATATATAAATCAAACCTCAGATCTCAAATAAGAGATCTAAGTAGATGAACCACTCCAAAAACCTACCTCACTTGAAAATTCAATGAGAAAGTGAGAGAAAGAGATAGAATCAAGAGCAACAAAATTCAAGCTAGAAATGAATTCGGATCCAATCTAATTGACCGACGGAAAGAGAAAAAGAGTAACAACAATAAAACAAAGAAAAAATCGTTATTCTCTCTCTCTTCCTCTACTAG
TARGET=582,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06146 Ps_contig_mira-and-tgicl-ass_franssen_41952
SEQUENCE=AAAATAAAATTTGAAGTTTCTGGCTACAGCTAACCCTTCTCTTTTCTCCTCCCTTCACTCTTAAAACGGAATTTAGGGTTTCTGCCCCCCCAAAAAATTCCAATTCCTTCCCCCAAACTCAACAATGGGTAAAAAGAAGCAGAAGGAGCCGCAACAAACCCCTAAAAACGACACCGTCTCATCCTCTTCCAGTGTTTCGATGTTCTCTTCGGCAACGCGCCGGAACAGATAACCGGCACTGCCGCTTCTCTTTTTTCCGACGACAACCCCTTTAAGAGAAAGCCTCCACAACCTGCTCTCATTGCTGATGTTACCGATAACACTCCAAATCCCGAAATCTGCGTTGTAGATGAGAAGAAGAGAAAGAGGAACAAAGAGAAAAGCCCCGTGCTTGATTCAAATTCTGCAATCGAAGTTTCTGAGAAGAAGAAAAAGAAGAAAAGTGGTTTGAACGAGAAAGAAGGGGAAGAGAAACAGCTTGTTTTGGATGTAGTAGCGAYTGGTCAGAAGAAGAAGAGGAAAAGGGAC
TARGET=506,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06147 Ps_contig_mira-and-tgicl-ass_franssen_41954
SEQUENCE=AACAACAGTAAACCTTCGGATTNGACTCACCAAAATCCATGACCCAAACCATGGAGGCGCTCCGATTATCCACCGCTCAACCTCACTTACCGCTCCTCCGTCGCCACCGTCACCACCACCACGGAATGCAGCGCACTACGCTTCAACCTAACCCTTGCTTCAAGCCTTTCATCGCCCCCGCGCTTTCCAAACCCGTAGAAATAGTTCCCTCTCCTTTACTCTCCGAGTCGCCACTGTCTCCTCCTCTTCCTCCTCCGTTCAAGGCTTCCACCGCTTCACTTCAGTACCCTCCAGGTTACGTCGGAGCGATTCCTGAACGGTCGCGCTCCGATGACGGCGATGCCTTAGTAAGTCCTATGAGCTATTTGACCAATATACTCACTTCTAAGGTCTATGATGTTGCTAAAGAGTCACCTCTTGAGTTTGCGCCGAAGATTTCTGAAAGAATTGGTGCTAACATTTGGCTCAAGCGGGAGGATCTTCAACCTGTATACTCATTTAAGATTCGTGGAGCTTATAATATGATGGCTAAACTTCCAGAGGAAGTTTTGGAGAAAGGGGTTATATGTTCTTCTGCTGGAAATCATGCTCAAGGAGTTGCCTTGTCTGCCAAGAGATTGAATTGCAATGCCGTTATTGCTATGCCCGTTACCACGCCTGACATCAAGTGGAAATCTGTGGAGAGGCTGGGTGCTACTGTTGTTCTTATTGGGGATTCCTATGACGAAGCACAAGCCTATGCAAAGAAGCGTGCTATAGAAGAGGGTCGTACATTCATACCTCCTTTTGATCATCCCGATGTCATTATGGGTCAGGGAACTGTCGGGATGGAAATTTTGCGTCAAATGAAGGGTCCAATTCATGCAATTTTTGTGCCTGTGGGAGGTGGTGGTCTCATTGCTGGTATTGCCGCTTACGTGAAGAGGATTAATCCTGAGGTGAAAATTATTGGGGTGGAACCCACTGATGCAAATGCAATGGCTTTGTCACTTCATCATGGCCACAGAGTGATTTTGGACCAGGTTGGAGGATTTGCAGATGGCGTGGCTGTTAAAGAGGTCGGCATAGAAACTTTTCGCTTGTGCAAAGAGTTGGTCGATGGTGTTGTTCTTGTAAGCCGTGATGCTATTTGTGCATCAATAAAGGACATGTTTGAGGAAAAAAGGAGCATCCTAGAACCAGCAGGCGCGCTTGCTCTTGCTGGAGCTGAGGCATACTGCAAGTATTATGGGCTCAAGGGGGAAAATGTTATAGCAATAACCTCCGGAGCAAACATGAATTTTGATAAACTTAGGACAGTAACTGAACTTGCTAACATTGGTCGTAAACAAGAAGCTTTGCTGTTAACGGTATTGCGGGAGGAGCCTGGCAGTTTCAAACAGTTTTGTCAATTGGTGGGGCCGATGAATATCACAGAATTCAAATACAGATATAATTCTGAGGAGAAAGCTGTTGTCCTTTACAGTGTTGGGATTCACACACCGTCAGAGCTAAAACAAATGGAGGAGAGAATGGGATCTTCTCAGCTTGTAACTCACAATCTAACAGATGTTGACTTGGTGAAAGATCATCTGCGTCACATGATGGGAGCCCGACCGGATATTCAGAACGAAGTTCTTTGTCGTTTTACCTTTCCAGAAAGACCTGGTGCTTTGATGAAATTTTTGGACTCCTTCAGTCCACGATGGAATATCAGTTTATTCCATTACCGTGCACAGGGTGAAACTGGAGCAAATGTATTAGTTGGAATTCAGGTTCCAAGCAATGAGATGGATGAATTCCATGACAGTGCCAACAAACTTGGGTATGATTATAAAGTGGTGATGGATGACCTTTTCTTCCAGCTTTTAATGCATTGATTTTGATAATTTTAGCCACTTGTCATTAAAAATCACTACCCTATATTTTATTGTGCCTCTATGTAGATATAATTTATCTAAATTTTAAGTTCACTACTACTCAACTTCTATGTTTTAAAAATATTTGAAATTTTGTGCTTTTAAAAGAAAATTTGTACATAAAAAAATGTCTGATTGAAATTGCTAGTTAG
TARGET=111,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1564
PRIMER_LEFT_PENALTY=0.125300
PRIMER_RIGHT_PENALTY=0.031122
PRIMER_LEFT_SEQUENCE=TNGACTCACCAAAATCCATGAC
PRIMER_RIGHT_SEQUENCE=ACCTGGAGGGTACTGAAGTGAA
PRIMER_LEFT=21,22
PRIMER_RIGHT=295,22
PRIMER_LEFT_TM=60.125
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.75
PRIMER_PRODUCT_SIZE=275
PRIMER_PAIR_PENALTY_1=0.1564
PRIMER_LEFT_1_PENALTY=0.125300
PRIMER_RIGHT_1_PENALTY=0.031122
PRIMER_LEFT_1_SEQUENCE=TNGACTCACCAAAATCCATGAC
PRIMER_RIGHT_1_SEQUENCE=AACCTGGAGGGTACTGAAGTGA
PRIMER_LEFT_1=21,22
PRIMER_RIGHT_1=296,22
PRIMER_LEFT_1_TM=60.125
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.3000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=276
PRIMER_PAIR_PENALTY_2=0.1726
PRIMER_LEFT_2_PENALTY=0.125300
PRIMER_RIGHT_2_PENALTY=0.047267
PRIMER_LEFT_2_SEQUENCE=TNGACTCACCAAAATCCATGAC
PRIMER_RIGHT_2_SEQUENCE=CGTAACCTGGAGGGTACTGAAG
PRIMER_LEFT_2=21,22
PRIMER_RIGHT_2=299,22
PRIMER_LEFT_2_TM=60.125
PRIMER_RIGHT_2_TM=60.047
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=54.545
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.2033
PRIMER_LEFT_3_PENALTY=0.125300
PRIMER_RIGHT_3_PENALTY=0.078029
PRIMER_LEFT_3_SEQUENCE=TNGACTCACCAAAATCCATGAC
PRIMER_RIGHT_3_SEQUENCE=ACGTAACCTGGAGGGTACTGAA
PRIMER_LEFT_3=21,22
PRIMER_RIGHT_3=300,22
PRIMER_LEFT_3_TM=60.125
PRIMER_RIGHT_3_TM=59.922
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=280
PRIMER_PAIR_PENALTY_4=0.2440
PRIMER_LEFT_4_PENALTY=0.125300
PRIMER_RIGHT_4_PENALTY=0.118715
PRIMER_LEFT_4_SEQUENCE=TNGACTCACCAAAATCCATGAC
PRIMER_RIGHT_4_SEQUENCE=AGGAGGAAGAGGAGGAGACAGT
PRIMER_LEFT_4=21,22
PRIMER_RIGHT_4=253,22
PRIMER_LEFT_4_TM=60.125
PRIMER_RIGHT_4_TM=59.881
PRIMER_LEFT_4_GC_PERCENT=42.857
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.3000
PRIMER_RIGHT_4_END_STABILITY=6.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=233
=
PRIMER_SEQUENCE_ID=SSR_06148 Ps_contig_mira-and-tgicl-ass_franssen_41978
SEQUENCE=GTTCGGGGCTCCCGNNGACGACGGCGCTATCTCGAAGTTAAAATGGAAGAAGAAGAAGAAGAGGAAATAAATCCTTTCACAAGCCTCCTAACCGACGACGATGATAAACCCATTGCATTCACTACCATACAAGACGATTTACCAAAGCAAGAATTGCTTCAAAAACATTTCCTCCGTTCGATTCAATCAACGGTTATAATTCGTCAGCTCCCATCGGAGGGTATCTCCTTCCAGCTCTGGCCCACCGCCACCTCTCTTGTCTCCCTCCTCGACAACCACCGCATAAATCCTACAAACAGCCCTCTCTCCACCGCCCTCGCAGCAAGCAACACTCCTCTGAGGATCCTGGAACTTGGCTCCGGCACCGGAATAGTCGGTATAGTCGCCGCCGCCACTCTCGGCAGCAACGTAACCCTAACAGATCTCCCTCATGTAGTTCCCAATCTTAAGTTCAACGCGGAAGCCAACGCGGGTGTTGTGGGGTCCAGCGGCGGGACAGTTACATTTGCACCGCTGAGGTGGGGCCACGCTGATGACGTGGAGATGATTGGGAGAGAGTTTGATGTTATCGTAGCTTCGGATGTAGTGTATCATGATCATCTCTATGAGCCGTTGATTGAAACGCTGCGTTTGTTGTTGATTAGGGAAAGTAAAATTTGGTTTGTTTTTGAGTTGGCTTACG
TARGET=46,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.1408
PRIMER_LEFT_PENALTY=1.030450
PRIMER_RIGHT_PENALTY=0.110393
PRIMER_LEFT_SEQUENCE=GGCGCTATCTCGAAGTTAAAAT
PRIMER_RIGHT_SEQUENCE=TGTTTGTAGGATTTATGCGGTG
PRIMER_LEFT=22,22
PRIMER_RIGHT=297,22
PRIMER_LEFT_TM=58.970
PRIMER_RIGHT_TM=59.890
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=1.1607
PRIMER_LEFT_1_PENALTY=1.030450
PRIMER_RIGHT_1_PENALTY=0.130244
PRIMER_LEFT_1_SEQUENCE=GGCGCTATCTCGAAGTTAAAAT
PRIMER_RIGHT_1_SEQUENCE=GGGCTGTTTGTAGGATTTATGC
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=301,22
PRIMER_LEFT_1_TM=58.970
PRIMER_RIGHT_1_TM=59.870
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=280
PRIMER_PAIR_PENALTY_2=1.1664
PRIMER_LEFT_2_PENALTY=1.030450
PRIMER_RIGHT_2_PENALTY=0.135999
PRIMER_LEFT_2_SEQUENCE=GGCGCTATCTCGAAGTTAAAAT
PRIMER_RIGHT_2_SEQUENCE=GTAGGATTTATGCGGTGGTTGT
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=292,22
PRIMER_LEFT_2_TM=58.970
PRIMER_RIGHT_2_TM=60.136
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=271
PRIMER_PAIR_PENALTY_3=1.2571
PRIMER_LEFT_3_PENALTY=1.030450
PRIMER_RIGHT_3_PENALTY=0.226649
PRIMER_LEFT_3_SEQUENCE=GGCGCTATCTCGAAGTTAAAAT
PRIMER_RIGHT_3_SEQUENCE=AGAGCTGGAAGGAGATACCCTC
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=237,22
PRIMER_LEFT_3_TM=58.970
PRIMER_RIGHT_3_TM=60.227
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=216
PRIMER_PAIR_PENALTY_4=1.3324
PRIMER_LEFT_4_PENALTY=1.030450
PRIMER_RIGHT_4_PENALTY=0.301926
PRIMER_LEFT_4_SEQUENCE=GGCGCTATCTCGAAGTTAAAAT
PRIMER_RIGHT_4_SEQUENCE=GTGGAGAGAGGGCTGTTTGTAG
PRIMER_LEFT_4=22,22
PRIMER_RIGHT_4=310,22
PRIMER_LEFT_4_TM=58.970
PRIMER_RIGHT_4_TM=60.302
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.2000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=289
=
PRIMER_SEQUENCE_ID=SSR_06149 Ps_contig_mira-and-tgicl-ass_franssen_41980
SEQUENCE=CGACAGAGTACGCGGGGNGGGGAAGTGACCTTCAGACTAACGCGGCCGGAAAGACGCTTCCTCCATAAGCACTTTTTCACTTTCAAACGCGAACGACACAACCTCCTGCGTTTTCGATGCTCCACACAGCTTCGGAAGTGAGAGGAGCCAACGCCAACGTCCGGAGGATCCACCATCGGATACGAAACAACACGACTCCGTCATTGCTGATATCCGTCAATGGCACGGACGCGATCTTAGACTATACGCACGAAGGTGAAGAAAACTCGAAAGCGAAGATGAAACGAAAGAGCTTACCAAGTGGAAGCGTCAACCTGAACTGGCGGATCTACGCTCTATCTTCTTGTTAGCTCGCGTCCCTTCCCTATGATGCGTTGCTTGCTGTTTCTTTGTTTGAAAAGAAGCGAAGATCGGAGACGAGTTAACGGATGAGATCGTCGGGATCGAGAAGAAGCGGTGGTGAACTCTTAGAATCTATCACTGCTACAGTCGCTGAAGGCGGAGGAGGTAGCGTTCAGTTATGGTGAAGTGTTGAGGTTCAATCAGGCATTCCGTTACAGGTTATGGTTGGTAGCGGTTATGATGAAGAGGAGTTCTTGGATGGATTCGCAGTGGTTGAAGGTTGAGGCTGAAGGATTGGGGGAGTTTGTGATTTCTGACTGCGATTTCGTCGTGACCGCACCGTTGCGAATTGGTATGCTCCTCTCTTTTCTCTGATTTCAGTGGAGTAAGAATTGAAAAAACGGTTTCGGTTGTTGACGATTGGAGTGTGGTGTAGGTGAAGGTTTGGTGAAGGTTTGATGAAGGTGGAGCTCACGGTTTCGGTTACGGTTCTGGTTGAAGACGATTTCCAGATCGTGATTGTGAAGTTTGTTAGAATTCCTTCGGATTGGTTGATGAGAGAGCGAGGGGGTTTTGGAAGCAGTGGTTCTTAAGAGGTTCGAATGGTTCAGAGTTGAGAGAAGGTCTTGGTGAATGAATGGAAGTTATGAAGGTTGATGTTCAATGGTGGTTGGTTGAAGCGATTGTGAAGTCATGGTTGTTAGAACGGTTGAGGGTTGTGAGAAGTGGTGGAGTTGATGGTGGTTTGAAGGTTGGTTGATGATGGTTGAAGATGATGGAGTGAAGTTTTGGTTGAAGAAGAAGAATCTCGGTTGGGAAGAGGAAGTTGAAGTGGTGGTTCTTGATGTTGAAGATTGGAGAAGAAAGTGAAGGTTACGGTTACGGTGATAGAAGGTGAAGACTATGGGGTTTGGTGAAGGAATTGAAGAGAGGAAGAAGAAACCGAATTTGAGAGAGGGAAGGAGAATTTATAGAGATGAGTTTTTTGGTTATAGTTTGTTAGTTAGTTGTGAATGAAAAATTCTGTTATTTCATTGTTGCAGGCTTTGCGGTTGGTGTCAATGCATTACCGTTGGATTTGGCTTATGTATTCACTGGAGCTTTGCTTATTCACCTGCGTTTTGCACCTTTTGCAGCTGGATCACAAGTCGTGGCTTACGCATTGGCTTGCGTTTGTTGCCGCGGTTTTAGCTTTGGCTTTGAGCTGCGTTTTTGAATGAGCTATTGTGAATGGCTTTGTGCTCTGCAGGTTGGAGCTTGGAAGTAGCTTTAGAAGCTTGGCTTGAAGCTTTGCACCGCTTGGTGAAGCTTGCTGCTTGTGTTTGGTTTTGGCTCAACAGTCTTTTTGGTGCATTTTATGCAGAATTTCTACATGCCTATTATGCAGTAATTTTTTGATGTGTTGCTGAATTTTTAATGCAGATTTTTTATGATGCAGAATTTTGTTTTAAATTATGCAGAATTTTATAATGCAGATGCAGAATTTTTATGATGCAATGCAGAATTTGGTTTATGCAAATGCAGTAATTTTGTTATATTGTAAATGCAGAATTTTGTTATGAATGCNAAGTTAATT
TARGET=1137,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0172
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.008930
PRIMER_LEFT_SEQUENCE=TGATGGAGTGAAGTTTTGGTTG
PRIMER_RIGHT_SEQUENCE=CGGTTTCTTCTTCCTCTCTTCA
PRIMER_LEFT=1115,22
PRIMER_RIGHT=1286,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.991
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=172
PRIMER_PAIR_PENALTY_1=0.1111
PRIMER_LEFT_1_PENALTY=0.102129
PRIMER_RIGHT_1_PENALTY=0.008930
PRIMER_LEFT_1_SEQUENCE=CGATTGTGAAGTCATGGTTGTT
PRIMER_RIGHT_1_SEQUENCE=CGGTTTCTTCTTCCTCTCTTCA
PRIMER_LEFT_1=1022,22
PRIMER_RIGHT_1=1286,22
PRIMER_LEFT_1_TM=59.898
PRIMER_RIGHT_1_TM=59.991
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.1636
PRIMER_LEFT_2_PENALTY=0.147295
PRIMER_RIGHT_2_PENALTY=0.016312
PRIMER_LEFT_2_SEQUENCE=TTCGAATGGTTCAGAGTTGAGA
PRIMER_RIGHT_2_SEQUENCE=GAACCACCACTTCAACTTCCTC
PRIMER_LEFT_2=939,22
PRIMER_RIGHT_2=1182,22
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=60.016
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=0.2292
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.220992
PRIMER_LEFT_3_SEQUENCE=TGATGGAGTGAAGTTTTGGTTG
PRIMER_RIGHT_3_SEQUENCE=TTCACCAAACCCCATAGTCTTC
PRIMER_LEFT_3=1115,22
PRIMER_RIGHT_3=1259,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=60.221
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=0.2359
PRIMER_LEFT_4_PENALTY=0.226971
PRIMER_RIGHT_4_PENALTY=0.008930
PRIMER_LEFT_4_SEQUENCE=AGTTGATGGTGGTTTGAAGGTT
PRIMER_RIGHT_4_SEQUENCE=CGGTTTCTTCTTCCTCTCTTCA
PRIMER_LEFT_4=1074,22
PRIMER_RIGHT_4=1286,22
PRIMER_LEFT_4_TM=59.773
PRIMER_RIGHT_4_TM=59.991
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=213
=
PRIMER_SEQUENCE_ID=SSR_06150 Ps_contig_mira-and-tgicl-ass_franssen_41983
SEQUENCE=AAAAGAAANATATCTGTAAATACCCAGATCGAATGTTGCTACCATTGCCACCAGCGAAGCAGTTGACGACGACTCTCAAAACCACTCTGACTTTTTAGGGTTCCGATCAACCACCAATGGCGGGTCGTAGAGATGCCTTACTCACCAGAGACAAAAACCATTCAATCACCGCATCTCGAATCGCCGTTGCCATTTTCGTCGGTGTTCTTCTTGGTTGTATCTTCGCTTTCTTCTCACCACATGGATTCTTCACTTCCATTCCAACTCCTCAAATTCTCAATCACAAGATGGCTTTGTCTGCTTGTGAATCGCCGGAACAACTTAGTGCGTTGAAGTTGAATATTCAATCTGCTAAAGAGAAGAATTCTGACTTGAAGAAGGAGGTGAAAAGTCTAATGGAAAGGTTGCGTTTGGCGGAGCAAGGAAAGGGTCATGCGAGGGAACAATTTGCTGTGCTTGGTGAGAATCACAAGGCTGGTCCTTTTGGTACGGTCAAGGCTCTAAGAACAAACCCTCCTGTTATTCCTGATGAATCAGTGAACCCTAGATTGGCAAAACTCTTGGAGGAAATTGCTATCTATAAAGAGGTTGTTGTTGTTCTGGCTAATACTAATGTGTTGGAAATGTTGCAAGTATGGTTCACCAATGTCAAGAGGGTTGGGATTCTTAATTATTTGGTTGTTGCATTAGATGACAGAATCGAAGAGTTTTGTAAGTCGAATGATGTTCCTGTCTATAGAAGAGATCCCGATCAAGGCCTTGATTTGATTGCCAAGTCGGGTGGGAACCATGCTGTTTCAGGATTGAAATTTCGTATTTTGAGAGAGTTTCTACAACTGGGTTACAGTGTTCTCCTCTCGGATGTTGATATTGTGTATTTGCAGAACCCCTTTGATTATATTTATAGGGATTCAGATGTGGAGTCAATGTCGGATGGTCATAGTAATCAAACGGCTTATGGATTTAATGATGTCTTTGATGAACCTTCTATGGGTTGGGCTAGATATGCTCATACCATGAGAATATGGGTTTATAACTCTGGCTTTTTTTACATAAGACCTACACTTCCTTCAATTGAGCTTTTGGACCGCGTTGCCGACCGGCTTGCTAAAGATCATAAAGCATGGGATCAGGCAGTTTTTAATGAAGAACTCTTTTTCCCTTCACATCCTGGTTATGATGGGCTTCATGCTGCAAAGAGAACAATGGATATGTACCTTTTTATGAACAGTAAGGTTCTGTTCAAGACTGTGAGAAAAGACGGCAAACTCAAGAAGTTGAAGCCTGTGATTGTGCATGTTAACTACCATCCTGATAAGCTTGAAAGGATGAAGGCTGTAGTCGAGTTCTATGTCAATGGGAAACAAGATGCGCTCGACAGTTTCCCAGATGGTTCTGATTAGAGCAGGAAATCAAAATCCACACGGTTCTAACTCAAAAAAGATTGGTATGCGCTAATTGTTGTGATACAGTGGAAGTCTTGTTAATTTATCTGAGGATCGATTTTATCTCATTGTACTTTCTGTTTTACAGTATGTCATATTTGATTATTAGACTAGTTTCTGTAATCTATCATCTTAACTTTGTATATCTCTTCTTGTGTGTATGTAGTTGGAATTTAATGTCCTCCGACAAACTAAGTTTGTTTTGTTTTTTCCTAGGAACT
TARGET=588,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0619
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.061184
PRIMER_LEFT_SEQUENCE=AGTGAACCCTAGATTGGCAAAA
PRIMER_RIGHT_SEQUENCE=TTAAGAATCCCAACCCTCTTGA
PRIMER_LEFT=535,22
PRIMER_RIGHT=669,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.939
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=0.1701
PRIMER_LEFT_1_PENALTY=0.108929
PRIMER_RIGHT_1_PENALTY=0.061184
PRIMER_LEFT_1_SEQUENCE=GCAAAACTCTTGGAGGAAATTG
PRIMER_RIGHT_1_SEQUENCE=TTAAGAATCCCAACCCTCTTGA
PRIMER_LEFT_1=551,22
PRIMER_RIGHT_1=669,22
PRIMER_LEFT_1_TM=60.109
PRIMER_RIGHT_1_TM=59.939
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=119
PRIMER_PAIR_PENALTY_2=0.1898
PRIMER_LEFT_2_PENALTY=0.128624
PRIMER_RIGHT_2_PENALTY=0.061184
PRIMER_LEFT_2_SEQUENCE=GGCTCTAAGAACAAACCCTCCT
PRIMER_RIGHT_2_SEQUENCE=TTAAGAATCCCAACCCTCTTGA
PRIMER_LEFT_2=496,22
PRIMER_RIGHT_2=669,22
PRIMER_LEFT_2_TM=60.129
PRIMER_RIGHT_2_TM=59.939
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=174
PRIMER_PAIR_PENALTY_3=0.1898
PRIMER_LEFT_3_PENALTY=0.128624
PRIMER_RIGHT_3_PENALTY=0.061184
PRIMER_LEFT_3_SEQUENCE=AGGCTCTAAGAACAAACCCTCC
PRIMER_RIGHT_3_SEQUENCE=TTAAGAATCCCAACCCTCTTGA
PRIMER_LEFT_3=495,22
PRIMER_RIGHT_3=669,22
PRIMER_LEFT_3_TM=60.129
PRIMER_RIGHT_3_TM=59.939
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=175
PRIMER_PAIR_PENALTY_4=0.2345
PRIMER_LEFT_4_PENALTY=0.173354
PRIMER_RIGHT_4_PENALTY=0.061184
PRIMER_LEFT_4_SEQUENCE=ACCCTCCTGTTATTCCTGATGA
PRIMER_RIGHT_4_SEQUENCE=TTAAGAATCCCAACCCTCTTGA
PRIMER_LEFT_4=510,22
PRIMER_RIGHT_4=669,22
PRIMER_LEFT_4_TM=59.827
PRIMER_RIGHT_4_TM=59.939
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=160
=
PRIMER_SEQUENCE_ID=SSR_06151 Ps_contig_mira-and-tgicl-ass_franssen_41986
SEQUENCE=TCTGAGATTAATTGAGAGACGAAACCGAGTTGATCAATGGATCAAATATGCAGGAATGCTATTGACTGTTGTTTCTGCTTGCCTTTGTTTCNGGAGATGATCAGGTCGGATGTTGGATAACTTATGTTTCTGTGTTATTGAGTAAAATTTNTATATAGGAAAAGTAAGACTTACCTTCCTTCCTTTCCTTAACGAAAAAAGTTNTTAGTTTGGTTTTTAGTTTTTTAACTTACCTTCAAGTTACGGTAG
TARGET=174,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06152 Ps_contig_mira-and-tgicl-ass_franssen_41990
SEQUENCE=TTGCCGTCAAAGAATAATTAGGGTTTGGTCCGGGTGGACAACAACCACCAAGACTCAGGGTAGGTTCCAAGGTTTCTAGATGACATGATGATTCTTGATTTTGNNTTTTTTTGGGGGTTCGATGAAGTTTTCTTAGATTCGTAACATCAGTGAGCATATATTTCTTTGTTTTTGTTTGNAATGTAAAGTTGATAGATGTCTTATTTTTTGGTAGATTATAAATTGTAGTAGTCGCTGATCATATTTATTTATTTGTTTGGCTTCACTGAGTCCATTGTTGGCAAGGATCCAAAAGATGTGATGCTTAACATCACAGGCAACACGATGAGACACTAGATACCATTATCGGTGTGATGTAAGCACAAATCATCCAGAACAATAGATAGGGTGTGCAAAGAAACGGTTATCTTTAATTAARTTGATATCCATTATCCCCGAAG
TARGET=242,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06153 Ps_contig_mira-and-tgicl-ass_franssen_42022
SEQUENCE=AAAGTTTAATCTCAAATTACTTTNGAAAAAACACACTGTGCGCTTATATAAACACATTGTGAAGAGACATGGAAATACATTAACCTGAAACAAAGATCTAATACACAATGACAATAGTTCTTGTTATTGGAAAAAACATGAACTAATGCATAAAAAATATATATTTTTTCATCCTCCTTGATGCTTACTCATCTTTTTATTTCATGTTTTCTACCAAACCATTTTCATTAGTAGAAATTTCGAATCAACCATAATCAACTGGTTTGGTAAGCACTGCCGATCATAAAATCATTATGATCGATAAACCTCAGGAAGCTGAAGACCGACACTAGCAGCAGATTTTCCAAGAGTCCTTTTAACCTCGTCTGATCTATCCACTGCAATGTTATATGAGAAAAGCAACTCCTTGTGATAGTGCAAGTTGTTAAGAAGGTAAGTGTAATCACGAGCAAGCTTTATTTTATGTTGGACAGCCACACCATCAGATAGATTTCGGTTCTTGTGGAACTCGGAGGTGACATATTCTAGAAAATGTCTCTTATTCTCTTCTTTCCCAATATGTTTCTTGACCGCCTTGAGAAGATGGCGATAGATGTTAGCAGTTCGGAAGGCTTCAGTTCTCATTCTTTTCCTATAAATATCCACAATATACTTCCTGAAACAAAAGAGATGTAAAAGGTGAAACGAGTTCCTAACTAGAACCTTACTCCGGGGCGGAAACGGTTTGCGCCGTCGCGATGATGCGTTTGCGTCAGAGGAAAGGAATGAAGATTGGCTGGGATTTTTTTTTGGTCCAGATTGGCTGGAATTATTATTATTATTNTATT
TARGET=808,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06154 Ps_contig_mira-and-tgicl-ass_franssen_42024
SEQUENCE=CTCTTTTCATCCTTAAATCCGAACTCAAAAATCGGCATTTACTATAAYAAACTTGACGCATACGCTTCCTATAGAGGACAACAAATATCTCTTGCAACTGGTGTTCCAGAAACTTATCAAGGTCACCGTGACATCTCCGTTTGGTCGCCGATGATATATGGTTTGGCTGTTCCGGTTTCACCTTACTTGTCTGAGATTCTGCGGCAGGATCTAACCTCAGGTGGTAGTTTTAGTCAACATTAAAGTCAACGGGAGAGTTAAGTGGAAGGTTGGGACTTGGGTTTCTGGAAGGTACCATATTGATGTGAACTGTCCGGCGTTTATACGGGTTGCCGGCGATAAAGGTGACGGGTTCGGAGTTTCTGGTCCGGCGGTTAAGTTTCAGTTATCACAAAGTTGTGTTGTTGATGTTTAAGTAATGTTTAAGCTAAGTAATTTGCTTTTATTATTATTATATTGTAATTCTAATTGTTATTGATTTTTAGGCTAAACTCTTAATTATCCTTTTAAACTA
TARGET=443,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06155 Ps_contig_mira-and-tgicl-ass_franssen_42027
SEQUENCE=AAAAAATAGTGAACYNNNAAGTGTTATAGAGGTTGGTGGTGGAGAGGGTAAAGGGGTAAGAGGAGAAGTTCTCAAAAGAATTAATAAAAGGGTGGTGGTGGTGGTGATGCATACGAGACTCCAAAGACTGGTGGTAATGGCCCTTCATATACAGGTGTAGGTGGTGGTGGTGAATTGTATTGAATTGGTGGTGGAGGTGAAGAATAGACTGGTGGTGGTGGTGGTGGTGAATTGTAATGAACTGGCGGTGGAGGTGAAGAATAGACTGTTGGTGGTGGTGGTGAGTTGTAATGAACTGGTGGTGGTGGAGAGGATAAACTGGTGCTGGTGGTGGAGAGGAATAGACTGGTGGTGGTGATGGGGACGGTGGTGGGAGATAAGGTGTTTGATGGGGAGGAGGTGGAGGTGATTGTTCTTCACAAGGAGGAGGTGGTGGTGGGGGTTCTATACAAGGTGGTGGAGATGGTGGTGGTGGTGAATGGACTGGTGGTGGAGGCGAGTGAACTGGAGGTGGTGGCGGTGATAAATACGGATAAACAGGTGGTGGTGGAGAGTGAGGTGGGGGAGGGAATGGAGGGGGTGGTGGGAGGGGAATGGAGGTGGTGGTGGAGGAGA
TARGET=91,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06156 Ps_contig_mira-and-tgicl-ass_franssen_42027
SEQUENCE=AAAAAATAGTGAACYNNNAAGTGTTATAGAGGTTGGTGGTGGAGAGGGTAAAGGGGTAAGAGGAGAAGTTCTCAAAAGAATTAATAAAAGGGTGGTGGTGGTGGTGATGCATACGAGACTCCAAAGACTGGTGGTAATGGCCCTTCATATACAGGTGTAGGTGGTGGTGGTGAATTGTATTGAATTGGTGGTGGAGGTGAAGAATAGACTGGTGGTGGTGGTGGTGGTGAATTGTAATGAACTGGCGGTGGAGGTGAAGAATAGACTGTTGGTGGTGGTGGTGAGTTGTAATGAACTGGTGGTGGTGGAGAGGATAAACTGGTGCTGGTGGTGGAGAGGAATAGACTGGTGGTGGTGATGGGGACGGTGGTGGGAGATAAGGTGTTTGATGGGGAGGAGGTGGAGGTGATTGTTCTTCACAAGGAGGAGGTGGTGGTGGGGGTTCTATACAAGGTGGTGGAGATGGTGGTGGTGGTGAATGGACTGGTGGTGGAGGCGAGTGAACTGGAGGTGGTGGCGGTGATAAATACGGATAAACAGGTGGTGGTGGAGAGTGAGGTGGGGGAGGGAATGGAGGGGGTGGTGGGAGGGGAATGGAGGTGGTGGTGGAGGAGA
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06157 Ps_contig_mira-and-tgicl-ass_franssen_42027
SEQUENCE=AAAAAATAGTGAACYNNNAAGTGTTATAGAGGTTGGTGGTGGAGAGGGTAAAGGGGTAAGAGGAGAAGTTCTCAAAAGAATTAATAAAAGGGTGGTGGTGGTGGTGATGCATACGAGACTCCAAAGACTGGTGGTAATGGCCCTTCATATACAGGTGTAGGTGGTGGTGGTGAATTGTATTGAATTGGTGGTGGAGGTGAAGAATAGACTGGTGGTGGTGGTGGTGGTGAATTGTAATGAACTGGCGGTGGAGGTGAAGAATAGACTGTTGGTGGTGGTGGTGAGTTGTAATGAACTGGTGGTGGTGGAGAGGATAAACTGGTGCTGGTGGTGGAGAGGAATAGACTGGTGGTGGTGATGGGGACGGTGGTGGGAGATAAGGTGTTTGATGGGGAGGAGGTGGAGGTGATTGTTCTTCACAAGGAGGAGGTGGTGGTGGGGGTTCTATACAAGGTGGTGGAGATGGTGGTGGTGGTGAATGGACTGGTGGTGGAGGCGAGTGAACTGGAGGTGGTGGCGGTGATAAATACGGATAAACAGGTGGTGGTGGAGAGTGAGGTGGGGGAGGGAATGGAGGGGGTGGTGGGAGGGGAATGGAGGTGGTGGTGGAGGAGA
TARGET=210,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06158 Ps_contig_mira-and-tgicl-ass_franssen_42027
SEQUENCE=AAAAAATAGTGAACYNNNAAGTGTTATAGAGGTTGGTGGTGGAGAGGGTAAAGGGGTAAGAGGAGAAGTTCTCAAAAGAATTAATAAAAGGGTGGTGGTGGTGGTGATGCATACGAGACTCCAAAGACTGGTGGTAATGGCCCTTCATATACAGGTGTAGGTGGTGGTGGTGAATTGTATTGAATTGGTGGTGGAGGTGAAGAATAGACTGGTGGTGGTGGTGGTGGTGAATTGTAATGAACTGGCGGTGGAGGTGAAGAATAGACTGTTGGTGGTGGTGGTGAGTTGTAATGAACTGGTGGTGGTGGAGAGGATAAACTGGTGCTGGTGGTGGAGAGGAATAGACTGGTGGTGGTGATGGGGACGGTGGTGGGAGATAAGGTGTTTGATGGGGAGGAGGTGGAGGTGATTGTTCTTCACAAGGAGGAGGTGGTGGTGGGGGTTCTATACAAGGTGGTGGAGATGGTGGTGGTGGTGAATGGACTGGTGGTGGAGGCGAGTGAACTGGAGGTGGTGGCGGTGATAAATACGGATAAACAGGTGGTGGTGGAGAGTGAGGTGGGGGAGGGAATGGAGGGGGTGGTGGGAGGGGAATGGAGGTGGTGGTGGAGGAGA
TARGET=270,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06159 Ps_contig_mira-and-tgicl-ass_franssen_42027
SEQUENCE=AAAAAATAGTGAACYNNNAAGTGTTATAGAGGTTGGTGGTGGAGAGGGTAAAGGGGTAAGAGGAGAAGTTCTCAAAAGAATTAATAAAAGGGTGGTGGTGGTGGTGATGCATACGAGACTCCAAAGACTGGTGGTAATGGCCCTTCATATACAGGTGTAGGTGGTGGTGGTGAATTGTATTGAATTGGTGGTGGAGGTGAAGAATAGACTGGTGGTGGTGGTGGTGGTGAATTGTAATGAACTGGCGGTGGAGGTGAAGAATAGACTGTTGGTGGTGGTGGTGAGTTGTAATGAACTGGTGGTGGTGGAGAGGATAAACTGGTGCTGGTGGTGGAGAGGAATAGACTGGTGGTGGTGATGGGGACGGTGGTGGGAGATAAGGTGTTTGATGGGGAGGAGGTGGAGGTGATTGTTCTTCACAAGGAGGAGGTGGTGGTGGGGGTTCTATACAAGGTGGTGGAGATGGTGGTGGTGGTGAATGGACTGGTGGTGGAGGCGAGTGAACTGGAGGTGGTGGCGGTGATAAATACGGATAAACAGGTGGTGGTGGAGAGTGAGGTGGGGGAGGGAATGGAGGGGGTGGTGGGAGGGGAATGGAGGTGGTGGTGGAGGAGA
TARGET=297,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06160 Ps_contig_mira-and-tgicl-ass_franssen_42027
SEQUENCE=AAAAAATAGTGAACYNNNAAGTGTTATAGAGGTTGGTGGTGGAGAGGGTAAAGGGGTAAGAGGAGAAGTTCTCAAAAGAATTAATAAAAGGGTGGTGGTGGTGGTGATGCATACGAGACTCCAAAGACTGGTGGTAATGGCCCTTCATATACAGGTGTAGGTGGTGGTGGTGAATTGTATTGAATTGGTGGTGGAGGTGAAGAATAGACTGGTGGTGGTGGTGGTGGTGAATTGTAATGAACTGGCGGTGGAGGTGAAGAATAGACTGTTGGTGGTGGTGGTGAGTTGTAATGAACTGGTGGTGGTGGAGAGGATAAACTGGTGCTGGTGGTGGAGAGGAATAGACTGGTGGTGGTGATGGGGACGGTGGTGGGAGATAAGGTGTTTGATGGGGAGGAGGTGGAGGTGATTGTTCTTCACAAGGAGGAGGTGGTGGTGGGGGTTCTATACAAGGTGGTGGAGATGGTGGTGGTGGTGAATGGACTGGTGGTGGAGGCGAGTGAACTGGAGGTGGTGGCGGTGATAAATACGGATAAACAGGTGGTGGTGGAGAGTGAGGTGGGGGAGGGAATGGAGGGGGTGGTGGGAGGGGAATGGAGGTGGTGGTGGAGGAGA
TARGET=464,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06161 Ps_contig_mira-and-tgicl-ass_franssen_42030
SEQUENCE=GTCGGGGATAATATTTACAGTTGTGTCATACACACTATTAAGAGTGTATCTAAGATGAAACCCCAAGTCCATCTCTCCGGTGAGAATTCTCCGACAGACGAGTTCAGAAAACCCGTAGGAGAGAAACTCAAACGGATTGATGACACTTCTAAGCAAGGCAGAGTTCAGATGTGTGATGAAATTGAGAGACGAAGATGGAGCCGCCGTGCTGAAGACTCTATTAGAACTTTGATGTTTTTGGGGTCATGGAGTCACACATGATTAACTAATCATTTATTCATTACGTTTGGTGTTAAAATAAAATAAAACTCCTGTTATATAAATAAGAATGGGTGGAAATTGAATGAGATTCTGTTTGTCTCATGTGTATATTGTAATTAATTAGTACTCTGTTTGTGGTAATGTAAATATATTAATAGCCTTATTTATT
TARGET=294,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3729
PRIMER_LEFT_PENALTY=0.022341
PRIMER_RIGHT_PENALTY=0.350587
PRIMER_LEFT_SEQUENCE=TCAAACGGATTGATGACACTTC
PRIMER_RIGHT_SEQUENCE=GAATCTCATTCAATTTCCACCC
PRIMER_LEFT=127,22
PRIMER_RIGHT=351,22
PRIMER_LEFT_TM=59.978
PRIMER_RIGHT_TM=59.649
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=225
PRIMER_PAIR_PENALTY_1=0.4959
PRIMER_LEFT_1_PENALTY=0.145354
PRIMER_RIGHT_1_PENALTY=0.350587
PRIMER_LEFT_1_SEQUENCE=AATTGAGAGACGAAGATGGAGC
PRIMER_RIGHT_1_SEQUENCE=GAATCTCATTCAATTTCCACCC
PRIMER_LEFT_1=179,22
PRIMER_RIGHT_1=351,22
PRIMER_LEFT_1_TM=59.855
PRIMER_RIGHT_1_TM=59.649
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.5037
PRIMER_LEFT_2_PENALTY=0.153161
PRIMER_RIGHT_2_PENALTY=0.350587
PRIMER_LEFT_2_SEQUENCE=GACAGACGAGTTCAGAAAACCC
PRIMER_RIGHT_2_SEQUENCE=GAATCTCATTCAATTTCCACCC
PRIMER_LEFT_2=92,22
PRIMER_RIGHT_2=351,22
PRIMER_LEFT_2_TM=60.153
PRIMER_RIGHT_2_TM=59.649
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=0.5151
PRIMER_LEFT_3_PENALTY=0.164560
PRIMER_RIGHT_3_PENALTY=0.350587
PRIMER_LEFT_3_SEQUENCE=GACGAGTTCAGAAAACCCGTAG
PRIMER_RIGHT_3_SEQUENCE=GAATCTCATTCAATTTCCACCC
PRIMER_LEFT_3=96,22
PRIMER_RIGHT_3=351,22
PRIMER_LEFT_3_TM=60.165
PRIMER_RIGHT_3_TM=59.649
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=256
PRIMER_PAIR_PENALTY_4=0.5151
PRIMER_LEFT_4_PENALTY=0.164560
PRIMER_RIGHT_4_PENALTY=0.350587
PRIMER_LEFT_4_SEQUENCE=AGACGAGTTCAGAAAACCCGTA
PRIMER_RIGHT_4_SEQUENCE=GAATCTCATTCAATTTCCACCC
PRIMER_LEFT_4=95,22
PRIMER_RIGHT_4=351,22
PRIMER_LEFT_4_TM=60.165
PRIMER_RIGHT_4_TM=59.649
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.9000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=257
=
PRIMER_SEQUENCE_ID=SSR_06162 Ps_contig_mira-and-tgicl-ass_franssen_42042
SEQUENCE=CCCTTTTTTAAAACTTTAAGCAAGGGAAAATAACAATTAATTAAANTTTAAAAAAGTAAAAAACTAATTGTAGTACATCTAACCTCAAAATTTTAAGTCATTCATCAAGCAAATGTTGCCTCAACTCAATTCTCCTGCTCTCAGAACTGGCACTCCCTCGTTTTCACCTATCCATTCTACCCTTACCAGCCTAGACCTTTAACTAATTAAAATTTGTAGGTAGATAATTTTTTTTTAAAAGGACCTACGTGCATTCTCAGTTAAATCATAACTCATCGTGACTTTCAGGTTGCTCGTCAACCTTTTCTTCACTTTCGCCTTTTGACGGGTTAGCAGATTCATCACTTTTAGAGGAACTATCAGTTGAGGTGGAATCAGAATTATCAGTAGTATGCTCATTGCTCTCACTTTCATTCTTTGTGGCCTTCTGAATCTTGGGCTTGGGCTTGGGGATTCTATTAATACTGGCGACCTTGTTTTGCAGTTCAAACACCTTTAGATGTACTTCTTCAGATGTAAATGCAGGCTTACTGAATCCAGAAGTTGTCTTTTGCTCAGTTACTTTCTCATCCAACCAATTCTTTAACTTTTCTGCAATATTAATCACCTCATCTACTCGTTCTTTTGGAAGCCAAGACTTCTTTGCTTTCCACTCTTCTACAATCTGTTTCAGCTCATTAATGTATTTGCGGGCATGCTCAACTGCTGCTGGTCGAGCTGTAAGCTCTTTTAACCTGAAGAAAATTGGATCTCCAACAGCTTTCAACTGATCCAGACGCCCTTCAAACTCTGTGGCATTGGCATCTTCACCATCTGTATACAACCAATCTTGCACCTCATTAAGCTTCTCAACAAAGGACTCACGTTCCTCACTCGTAGAAACTTTTTCAAACTCCTCAAGAGTCTCAATCTTTTCTTTAGTAGTATATATATATCCTTCTAAATTATTTTTAGACTCGGCTGTCCGTTTCCTTTCTGCGTCTTTTTGTCTAGTGCTTGTAATTTTGTTTTAGCTTCAGCAAGAAAATCTTCCGATAGAGACATCCCCGGTCCTGTAGTCTTCTCAACAATCTTTAATGGTACCCTAAAGGTCCGTTTTTTCAGCTTTCTCTCTGTAGCAGGTTCAGCAGGAGCTTGCTCTTCTGCACTCGTATTAGATGTCTTACTAATACCACTATCACTTTGCACACTTTCATTGTTTTCGTCAGATTTGTTACTAGCACCAGATTCATCAGATACATTTGACGAAATAGTTGAATTCTCTACGGTCAAGTTCTTCCGGGGAACTTCCACCCACTCTGTTATTTCAACAACAGCGTCTGCCCGATCCAGAGAAAGAACTCCACTTCTACTAAGAGAGAAATGTACATTTGCCTTAATGGGGGACGACAGATTCCGAGATGAGTATTTGCCACTTGCATCTGTTAAACCAGATTATTTGGTATTGAGCAAATTAAAGGAGAGGTTACACCGAGGTGRCAAAAGATGTTCACTTT
TARGET=925,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06163 Ps_contig_mira-and-tgicl-ass_franssen_42063
SEQUENCE=AGGTAGTTACATTCTAATCAAAGTGAAGTGAAGGTGTTAGGTTGAAGGAGGAGTTACACTACACTACACAAGAAGCAAAGAAAAAAAATTGGCGGCGTGTTCATCTTCATCTTCATCACCGGCGACGGAGGAAGAGATAACACTGAAAGTGAAATGGACCGGAAAGGAATACACCGTTCGAGTGTGCGCCGACGACACTGTCGGAGAACTCAAGCGTCGTATCTGCGAAGTAACCAACGTTTTACCCATTCGTCAGAAGCTTTTGTACCCTAAACTCGCTTCTAAACTCAACGATGATTCTCTCTTACTCTCTCAACTCTCTATCGATCTCAACAAGTCGTCTCTCAAATTCACCATGATCGGTACTACTGAAGAGGATTTGATAGTTGATCCAGTGGATTCCCCTGAGATTCTTGATGATTTTGAACTTGCTCAAGAAGAAGCTATTGATACCAAAGATATGCAAGTTAACAAACAGAAATTGACCAGACGCATTAATCACTTCAAAGTTGAGATTCAAAACCCTTGTCGCCAAGGAAAGAAACTCCTTGTTCTGGATATTGATTACACTCTTTTTGATCATCGATCTACCGCGGAAAACCCTCTTCAACTCATGAGACCTTACCTTCATGAGTTTCTAACTGCAGTTTATGCTGAGTATGACATTATGATATGGTCTGCGACAAGTATGAAATGGATTACCCTCAAGATGAGCCAACTTGGGGTTCTGGACAATCCTAACTACAAAATCACAGCATGTCTTGATCACTTGGCAATGATCACTGTCCAGACAGCTTCTCGTGGGGTCTTTGATTGCAAGCCACTTGGTTTGATCTGGGCTCAGTTCCCTGAGTTCTACAGTGCTTCTAATACTATTATGTTTGATGACCTTCGGAGAAATTTTGTGATGAACCCACAGAATGGTTTGACAATTAAACCGTTCAGGAAGGCTCATGCAAACCGGGACAGTGATCAGGAACTTGTGAAACTTACCCAGTACTTACTAGCCATTGCAGAGCTTGATGATTTGAGTCACCTTGATCATAATAAATGGGAGTCATTCTCGGAGGACACTGGTAAAAGGCGTAGGCACAATTGAGTGATAAATAGAAAACAATAACAGGAATTTGGTAAGCAAGATGAAGCTTCTAACCTTTATATACAAAACTTGGATCAAAGCGGGGTTTTGGGGTTGTTTCCTTATTATATTGTTACCAAAGAAATGTTAAAATGCACTATAAACTGAGAAAATTGGTGACAAGTGACACAACTATAACTAGCAGCCGAAGTGATTAATTTATTCTATTTCTGAACAATTGAATTGCCTTATAAGAAAGGTTGTTGTTTGTGGCTTT
TARGET=55,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8636
PRIMER_LEFT_PENALTY=0.856390
PRIMER_RIGHT_PENALTY=0.007205
PRIMER_LEFT_SEQUENCE=AAGGTGTTAGGTTGAAGGAGGA
PRIMER_RIGHT_SEQUENCE=GCTTCTGACGAATGGGTAAAAC
PRIMER_LEFT=30,22
PRIMER_RIGHT=259,22
PRIMER_LEFT_TM=59.144
PRIMER_RIGHT_TM=60.007
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=0.8636
PRIMER_LEFT_1_PENALTY=0.856390
PRIMER_RIGHT_1_PENALTY=0.007205
PRIMER_LEFT_1_SEQUENCE=GAAGGTGTTAGGTTGAAGGAGG
PRIMER_RIGHT_1_SEQUENCE=GCTTCTGACGAATGGGTAAAAC
PRIMER_LEFT_1=29,22
PRIMER_RIGHT_1=259,22
PRIMER_LEFT_1_TM=59.144
PRIMER_RIGHT_1_TM=60.007
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=231
PRIMER_PAIR_PENALTY_2=0.8742
PRIMER_LEFT_2_PENALTY=0.856390
PRIMER_RIGHT_2_PENALTY=0.017846
PRIMER_LEFT_2_SEQUENCE=GAAGGTGTTAGGTTGAAGGAGG
PRIMER_RIGHT_2_SEQUENCE=TCATCGTTGAGTTTAGAAGCGA
PRIMER_LEFT_2=29,22
PRIMER_RIGHT_2=296,22
PRIMER_LEFT_2_TM=59.144
PRIMER_RIGHT_2_TM=60.018
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=268
PRIMER_PAIR_PENALTY_3=0.8742
PRIMER_LEFT_3_PENALTY=0.856390
PRIMER_RIGHT_3_PENALTY=0.017846
PRIMER_LEFT_3_SEQUENCE=AAGGTGTTAGGTTGAAGGAGGA
PRIMER_RIGHT_3_SEQUENCE=TCATCGTTGAGTTTAGAAGCGA
PRIMER_LEFT_3=30,22
PRIMER_RIGHT_3=296,22
PRIMER_LEFT_3_TM=59.144
PRIMER_RIGHT_3_TM=60.018
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=0.9178
PRIMER_LEFT_4_PENALTY=0.856390
PRIMER_RIGHT_4_PENALTY=0.061436
PRIMER_LEFT_4_SEQUENCE=AAGGTGTTAGGTTGAAGGAGGA
PRIMER_RIGHT_4_SEQUENCE=GGTAAAACGTTGGTTACTTCGC
PRIMER_LEFT_4=30,22
PRIMER_RIGHT_4=245,22
PRIMER_LEFT_4_TM=59.144
PRIMER_RIGHT_4_TM=59.939
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=10.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=216
=
PRIMER_SEQUENCE_ID=SSR_06164 Ps_contig_mira-and-tgicl-ass_franssen_42097
SEQUENCE=CTTTGGATATCAAAGGCTTTTTCTTTGGGACAAGCCCACCATACTTCTTGCCCGCAGATGATGATGACTCAACGTCATTGGCAACGAAATAGTCTTCTCTATTACACTCTGCCATCTCTCTTTACTCAAAGCAAGATAGTAAACGACAAGGTTAGAGAAGAGAGAGAGAAGAGGATATAGAAACAGAGAAATCTCCAAAATCAAGACAAAGAAGTAAATTTCTGTCGT
TARGET=159,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06165 Ps_contig_mira-and-tgicl-ass_franssen_42098
SEQUENCE=CTTATCCCAAATGCCAGGGGGGTATGTCCGAAACTTGAGGGAGTGCGGTCCAGTAAACCGCGTGGGCTTAAAGAAGCTCATAAGCCCAACCTGTTATCGTCAAGGTTAACCACAACACGCAGGAGAAAARAGAGGAAACGGAAGAGCACATCACCGACGCTCTCTCCATCATCAGAGCGAAATGAAATGCGATCCCGTCGCCGCCTCCATTTCGTAACTGAAAACGCAAGATCACCTAACAGAACGCTAACACACACTCGATCCAACGATTACACCACGCGGTCAACACAAATCTGGCTTGATGATGAACGGATCTTCAAGCTTCGCGATCCAGAAGGCAGCTTCCGTCACCGGCAGCAGCGATCCATTCCAACAACTAATCCGCGCTCCTCAACCAACCTGAAACAAAGATAATCGGCCAAATAGGAGCAACCGCGAGCGGTAAAAGCAAGATCGAAGGTTTCGTCGGCGGCTTCACTTTGAAATTCCGGTAACCTCCTCGAATTGGAACTGCAAGATCTGACACGGTGGCTTTCCGACGATAACCGGTGTGGAATTGGCTTTTGCTGGCTCGCGTTTAGCTGCGGTTTGCCGGCTTAATTGTAACTGTAGCTTATTTGGTGCTTGGAAGTAGCTTGGCTTGTAGTTGGAATTGCATCGCGTTTTGGCTTGGCGTGTTGTAGCAGGTGTTGTGCGTTTGGAATGCTGTGTTTGGCTGCGTTTCAAGTTGTGCTCATGGCTTGGCGCTGGCTTTGGTGAAGCTTACGCGTATCCGTTGTTTTGGAGCTTTGGATGCATTCTTTTGAAGCTTACGCTCAGGTCTTTTCACTTGCAAGAACTTATTTATTCTCACTTGTTCCTTTTTCCTCCTATTGCTTANTTACTTTATTTATTTATTTATTTTATTTTTATGACAAACTACATGCTA
TARGET=885,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06166 Ps_contig_mira-and-tgicl-ass_franssen_42115
SEQUENCE=CNGTACTGTGCTCGTCTCTTCCTCCTCGTAACCTTGATCATCGGCTAACTTTGGGATGTTTTTTGACTTGCCCGTCACTCGATCTCTTGGTGCTGCCCCGAGATTTGCTTCCACTGTCTACAGTGGGCTTGTTGTCCTTTACTGGCTTCCTGTCGGTAACAACTTCAAACACAGTTGGAAGCTCATTGATCAAGCTAAATAGACGTTTCCTTTCATTGCGGTTAAGTCGAGCTCCGAGATAAAATGCCACAGAAAGCAACCATGAATCACTGTGCACCGCAACAAGAGAAAGCCAATCCCTACGGTTCATGCCATCCCTTGCAAAGTTGATTCCGAGAGCCGGCTCTGGCAGCTCAGGTGGAACCTCCTCGGCTGGAAGTGTCACTTCCCATGATTCATTTGAATGTCCATACAGGCACAAGTTATCCTTATCTGGATCACATAGAGTGTAGAATTCATCAACATCTTGAGTTAAAGCACGAACGATACCGTCTCTACGAGCACTGAAATCTTTGAAGATCTCTTCAACGGAGCGAGGACTTGACGCCATTTCCATAAAACCTAATTTGAGATCTACGCGATTGGGTTCAGTTTCGAGAAGATTCTGAAGAGTGAGATCGACGGTGAGGATGAATTTGAGGTTTGAAGGAAGTGGCTAAACCATGGGAGAGGATCGATCGCAATGAAGATGCATGTGATGGATGATGAGAGAGAGAGAGAGACTAAGGTTGGAAAATTATNTANNCCCG
TARGET=707,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06167 Ps_contig_mira-and-tgicl-ass_franssen_42117
SEQUENCE=ATATATAATATATCATTCTTCTTCTTCTTCAATAATTCACATACACCAATTGCTTTTGTTTATGTGATTGAAAATGGATTGTGTGATGAACAGCTTTGGCGGCGATTGGAGAGAAGGAATGGTACTTTTATGTGCCTCGAGATCGAAAGTATAGAAACGGTGATCGTCCTAATCGCGTAACAACTTCTGGTTATTGGAAAGCTACCGGAGCTGATAGGATGATTAGAACTGAAATTTCCGATCAATTGGACTGAAGAAAACCCTAGTTTTCTATTCTGGTAAAGCTCCTAAAGGTATAAGAACTAGTTGGATTATGAACGAGTATCGCTTACCTCAACACGAAACCGAACGATATCAAAAGGGTGAGATATCGCTGTGTCGCGTATACAAGAGAGCTGGAGTAGAAGATCATCCTT
TARGET=16,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06168 Ps_contig_mira-and-tgicl-ass_franssen_42161
SEQUENCE=GYCNCTTTTCTTCCCTTTTTCTACTCTCAAANCTTTTCTTCTACTTTACTTTCCGTCCATCATCAAAACGACTCTGTGTCAAGAGAGTGTCATAGTGATACTGAAACGCTGCACAACGTTTCTTCTTTCTCTTATGGCATGCCCCAAGAGGACCAAGATCAATTTTGGATCACTCAATTCCTCTAGTTCATCATCATCATCATGATTTTCCGGTTCTTTCACCCTTTCCTTCTTCTTCTCTTCACTCAATTTGTGTTGCTGCAAGCGTTCACGGGAACGTATGGTATAAATTACGGGAGGATTGCAGATAATATTCCCTCGCCGGATGAAGTTGTTACTCTTCTCAGAGCTTCGAAGATAAGGAACATTAGAATCTACGATGCTGATCACAGTGTTCTCAAGGCTTTTAGCGGAACCGGTCTTGAAATTGTTATTGGAGTTACCAATGGACAGCTGCAAGACATGAGTTCGAGTGCGGATCATGCTTTGAACTGGGTGAAAGAAAACGTGCAGGCGTTTCTTCCCGAGACACGGATTCGTGGAATTGCTATTGGGAATGAAGTTTTGGGTGGGTCTGACAATGCTTTGTGGGGGGTTCTTTTGGATGCTGCGAAAAATGTTTATAATGCTACGAAGAAACTTCATCTGGATAAGATAATTGAGATTTCTACCGCTAACTCGTTTGCTGTTTTTCAAGATTCTTACCCTCCATCCTCTTGTAAATTTGACAATAATGTTAAGCAGTACATGAAGCCGCTATTAGAGTTCTTCCAACAAATTGGATCTCCTTTCTGTTTGAATGCATACCCTTTCCTAGCTTATGTGAGTAATCCAGAGGATATTGATATAAATTATGCTCTTTTTCAACCAACAAAGGGAATTTATGATCCAAAGTTTAAACTGCATTACGATAACATGCTCGATGCTCAGATCGATGCAGCTTATGCTGCTTTGGAGGATGCTGGATTTCATAAAATGGAAGTCATAGTTACGGAGACAGGGTGGGCCTCTGCAGGAGACCAAACCGAACCTGGAGCAAACGTGACTAATGCGAGGACGTATAATTATAATTTGCGCAAGAGGCTTGCAAAGAAGAAAGGAACCCCGCATAGGCCTAAAAATGTTGTAAAGGCATACATTTTTGCAATCTTCAACGAGGATTCGAAGAACGGTCCTACCTCCGAGAGGAATTATGGATTGTTCAAGGCTGATGGGAGCATAT
TARGET=188,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06169 Ps_contig_mira-and-tgicl-ass_franssen_42195
SEQUENCE=CCCGCCCCGGACCGGACGGAAACGAATACCGCCGACGAAGACTCGTCGCATAAACGTACTCCCAAAACCTACCTTCTTCCGCCAAATAACGTCCCTTTCTAACGCCGATCCGTCTTCCACCGAAACCTACTCCCTAGACAAGATCGTTTATCGATCCCAATCCGGGGGACTCCTCGATGTCCAACACGACCTCGGCGCGCTCAAGGAAATTCGATGTGTGAAGTATTTTAGAAGGCCATCAACACAACTTTGAAGAAGTACTTTTGTTAGAAATTGGTTGGTTGGTCACCTGATGGAAGCAAGGTCACAGCGT
TARGET=274,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9486
PRIMER_LEFT_PENALTY=0.110392
PRIMER_RIGHT_PENALTY=1.838183
PRIMER_LEFT_SEQUENCE=CTCAAGGAAATTCGATGTGTGA
PRIMER_RIGHT_SEQUENCE=CTGTGACCTTGCTTCCATCAG
PRIMER_LEFT=199,22
PRIMER_RIGHT=309,21
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=60.838
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=111
PRIMER_PAIR_PENALTY_1=2.0600
PRIMER_LEFT_1_PENALTY=0.221785
PRIMER_RIGHT_1_PENALTY=1.838183
PRIMER_LEFT_1_SEQUENCE=GCATAAACGTACTCCCAAAACC
PRIMER_RIGHT_1_SEQUENCE=CTGTGACCTTGCTTCCATCAG
PRIMER_LEFT_1=47,22
PRIMER_RIGHT_1=309,21
PRIMER_LEFT_1_TM=59.778
PRIMER_RIGHT_1_TM=60.838
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=2.1715
PRIMER_LEFT_2_PENALTY=0.333282
PRIMER_RIGHT_2_PENALTY=1.838183
PRIMER_LEFT_2_SEQUENCE=CTCCCAAAACCTACCTTCTTCC
PRIMER_RIGHT_2_SEQUENCE=CTGTGACCTTGCTTCCATCAG
PRIMER_LEFT_2=58,22
PRIMER_RIGHT_2=309,21
PRIMER_LEFT_2_TM=60.333
PRIMER_RIGHT_2_TM=60.838
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=252
PRIMER_PAIR_PENALTY_3=2.1905
PRIMER_LEFT_3_PENALTY=0.352345
PRIMER_RIGHT_3_PENALTY=1.838183
PRIMER_LEFT_3_SEQUENCE=CCGAAACCTACTCCCTAGACAA
PRIMER_RIGHT_3_SEQUENCE=CTGTGACCTTGCTTCCATCAG
PRIMER_LEFT_3=119,22
PRIMER_RIGHT_3=309,21
PRIMER_LEFT_3_TM=59.648
PRIMER_RIGHT_3_TM=60.838
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=191
PRIMER_PAIR_PENALTY_4=2.2290
PRIMER_LEFT_4_PENALTY=0.390771
PRIMER_RIGHT_4_PENALTY=1.838183
PRIMER_LEFT_4_SEQUENCE=CAAGGAAATTCGATGTGTGAAG
PRIMER_RIGHT_4_SEQUENCE=CTGTGACCTTGCTTCCATCAG
PRIMER_LEFT_4=201,22
PRIMER_RIGHT_4=309,21
PRIMER_LEFT_4_TM=59.609
PRIMER_RIGHT_4_TM=60.838
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=52.381
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=109
=
PRIMER_SEQUENCE_ID=SSR_06170 Ps_contig_mira-and-tgicl-ass_franssen_42199
SEQUENCE=CTCGTTTATGCNTTTATTTATTTACGCCAGTGAAAGGGGTTCTCGTACCCTACCGACGTTCTTTGAAACCGCCACCTACCTGCATATGTAACCATTCTGGGGATGATCCTCTGCTTGGCTCTCTTGTAGCTTGTGAAACAAATGGCATGCCGGCTAGATCATATGTTTGTTGACCATAATCTTTTGGAGGAAAGACTTCAATGCCAGTGTTGAGTCTCGAATTAACTGCTTCAAGTTTCATTGACAAAACTGTGACATCCAGTGACATTACAATTCTCAAAAATCAAATTACAAAACATATTAATTTTTTTTACAAAGTATTAATAA
TARGET=13,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06171 Ps_contig_mira-and-tgicl-ass_franssen_42204
SEQUENCE=GTATTCACTAATAGCTTTATGCAGCACACTGTTGAGTTTAAAAGACCATTTGAGATCTTCATCGATAACTTCCTCGTACCATGTGGAATCATCAGCATCACCAACAACGTTGTTGTTGTTGTTGACATTGGTTTTGTTGAGGTTCTCCCATTGGTTTTTTTGTTTTTAAGAAAAAAACCCACCTCCCATTTGTTAGGAAAAAATAAAAAAAGATGAATGAATAGAAAAGAAACAATGAAGAAGTGCAATCGTCCCCGCGTACTCTGCGTTNACTACC
TARGET=109,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3440
PRIMER_LEFT_PENALTY=0.134318
PRIMER_RIGHT_PENALTY=0.209637
PRIMER_LEFT_SEQUENCE=AATCATCAGCATCACCAACAAC
PRIMER_RIGHT_SEQUENCE=TTTTCCTAACAAATGGGAGGTG
PRIMER_LEFT=86,22
PRIMER_RIGHT=200,22
PRIMER_LEFT_TM=59.866
PRIMER_RIGHT_TM=60.210
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=115
PRIMER_PAIR_PENALTY_1=0.4310
PRIMER_LEFT_1_PENALTY=0.221368
PRIMER_RIGHT_1_PENALTY=0.209637
PRIMER_LEFT_1_SEQUENCE=TACCATGTGGAATCATCAGCAT
PRIMER_RIGHT_1_SEQUENCE=TTTTCCTAACAAATGGGAGGTG
PRIMER_LEFT_1=76,22
PRIMER_RIGHT_1=200,22
PRIMER_LEFT_1_TM=60.221
PRIMER_RIGHT_1_TM=60.210
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=0.4527
PRIMER_LEFT_2_PENALTY=0.243031
PRIMER_RIGHT_2_PENALTY=0.209637
PRIMER_LEFT_2_SEQUENCE=ACTTCCTCGTACCATGTGGAAT
PRIMER_RIGHT_2_SEQUENCE=TTTTCCTAACAAATGGGAGGTG
PRIMER_LEFT_2=67,22
PRIMER_RIGHT_2=200,22
PRIMER_LEFT_2_TM=59.757
PRIMER_RIGHT_2_TM=60.210
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=0.7117
PRIMER_LEFT_3_PENALTY=0.502108
PRIMER_RIGHT_3_PENALTY=0.209637
PRIMER_LEFT_3_SEQUENCE=AGCTTTATGCAGCACACTGTTG
PRIMER_RIGHT_3_SEQUENCE=TTTTCCTAACAAATGGGAGGTG
PRIMER_LEFT_3=12,22
PRIMER_RIGHT_3=200,22
PRIMER_LEFT_3_TM=60.502
PRIMER_RIGHT_3_TM=60.210
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=189
PRIMER_PAIR_PENALTY_4=0.7158
PRIMER_LEFT_4_PENALTY=0.506150
PRIMER_RIGHT_4_PENALTY=0.209637
PRIMER_LEFT_4_SEQUENCE=TAACTTCCTCGTACCATGTGGA
PRIMER_RIGHT_4_SEQUENCE=TTTTCCTAACAAATGGGAGGTG
PRIMER_LEFT_4=65,22
PRIMER_RIGHT_4=200,22
PRIMER_LEFT_4_TM=59.494
PRIMER_RIGHT_4_TM=60.210
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=136
=
PRIMER_SEQUENCE_ID=SSR_06172 Ps_contig_mira-and-tgicl-ass_franssen_42210
SEQUENCE=AGTACGCGTGACTGGAATTGAAAAACGAAAAACGAAGGGGTAATAGGAGAATTAGGGTTTTGCTCTGGGAGGGAAAATTATAATTCTAAAATCCTTAAATCAGAATTTCAACTCATTGAGATCCACATTCAAAATTCAAAATCGCCACCATCGCCACCAGATATTAACTCTACTCCCTGAAATATAATATAATATAATAACATGAGCACCACAGTGCAAAGAACTCCAAGGTCTGGCAGACAGTCATTGTTTTTCCAGGATTTAGCCTCCCCTCTTTCTGGCCGCCGGGGGAAGTTTTCTAGTCCTGGTCAGTCAGGCGCTTCTTCTTCTCTCTGGCGCGAAAACTTTGGTAATTCAGACCTTCCTCCGCCTCCTGTTTTCACCTTGGATGACAGATCAGACTATTCTCCTGAATCAGGCATATTGGATTACCAAGTATCTCCTGAGACTAAGTCGGAGAACAAAACTCCTGTGCAGAATTTAAATAGGGAATTTTCAACTCCAGCCAAAGGCAAATCCGAGGCCAGCACATCTTATGCTTTAAGAGGGGTGCAGCAAAACCAACAGGGCTCGCCAGGTTTGAATTGGTGGTCACCTGCAACTGCAAAGAGTGGTGGTGATCAAGACGAGAAAGGAAGGAGTTCTCCAGTGGAGGGGGTGGTTCAGCCTGGTGCTTTGATTACTCTTCCTCCACCACTAGAAGTAGCAAGGCCTGAGGTTCAGAGAAATTCCTTGCCTGCAGGGAATCTCAATGAGGAAGAATGGGTAACAGTATATGGATTTTCTCCATATGATACTAATTTGGTTTTAAGGGAGTTTGAAAAATGTGGTGAAATTTTGAAACATGTTTCTGGTCCTAGAGAGGCTAACTGGATGCACATTTTGTATCAGAATCGGTCGGGTGCACAAAAAGCTCTTAACAAGGATGGAATACAAATTAACGGAGTCCTAATTGTTGGTGTGAAACCCTTAGATCCCATGCAACGACAAGCTTTAAATGAGAGATTGAACAATCAAGGATTTATACCCTTACCTCTACCCTCTGCCAGAAACTCAGAAGCAAGCACATCAAAAGCTCTTTCTCGGCCATATTATCTGCAGAACGGCAACACATCTACAAGGCAGACTGGAGGTACCATTGCTTCCCCAACAAAATCATTGGCGTCCAAGATCATTGATTTAATGTTTGGAGTCTAGATCAGCTGTTCTGGTTAATCAAACTTTATCATCCTGTTTTGTTATAAGCAATATTTGACTCGGTTATTCACACCCTCTAAAGAGGTTCGGCGTGTTATGTTGGATTTGAGTTGGAATATTACTCATTTAATTGAATTTTATGGCATCTTAGCATTGTGATCTTATGAATGGCTACTAGTTGCCAAATGTTTTGAGAAAGGTTCATAATTTATGGGTACAGGTTGATCAATGTTAATCTGATCATTGTGCAAACGCTTCAGATTTTGGACAGAATTGAATGTAATGTCAGATTTGTTTAANNCATGGAGCTCACTTTGATATTTGTTTTTG
TARGET=181,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2134
PRIMER_LEFT_PENALTY=0.102276
PRIMER_RIGHT_PENALTY=0.111077
PRIMER_LEFT_SEQUENCE=ACCATCGCCACCAGATATTAAC
PRIMER_RIGHT_SEQUENCE=CTGATCTGTCATCCAAGGTGAA
PRIMER_LEFT=146,22
PRIMER_RIGHT=399,22
PRIMER_LEFT_TM=60.102
PRIMER_RIGHT_TM=60.111
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=254
PRIMER_PAIR_PENALTY_1=0.2280
PRIMER_LEFT_1_PENALTY=0.102276
PRIMER_RIGHT_1_PENALTY=0.125755
PRIMER_LEFT_1_SEQUENCE=ACCATCGCCACCAGATATTAAC
PRIMER_RIGHT_1_SEQUENCE=ACCAGGACTAGAAAACTTCCCC
PRIMER_LEFT_1=146,22
PRIMER_RIGHT_1=308,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=59.874
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=10.9000
PRIMER_PAIR_1_COMPL_ANY=2.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=163
PRIMER_PAIR_PENALTY_2=0.2563
PRIMER_LEFT_2_PENALTY=0.102276
PRIMER_RIGHT_2_PENALTY=0.154023
PRIMER_LEFT_2_SEQUENCE=ACCATCGCCACCAGATATTAAC
PRIMER_RIGHT_2_SEQUENCE=GAAAAACAATGACTGTCTGCCA
PRIMER_LEFT_2=146,22
PRIMER_RIGHT_2=254,22
PRIMER_LEFT_2_TM=60.102
PRIMER_RIGHT_2_TM=60.154
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=109
PRIMER_PAIR_PENALTY_3=0.2563
PRIMER_LEFT_3_PENALTY=0.102276
PRIMER_RIGHT_3_PENALTY=0.154023
PRIMER_LEFT_3_SEQUENCE=ACCATCGCCACCAGATATTAAC
PRIMER_RIGHT_3_SEQUENCE=TGGAAAAACAATGACTGTCTGC
PRIMER_LEFT_3=146,22
PRIMER_RIGHT_3=256,22
PRIMER_LEFT_3_TM=60.102
PRIMER_RIGHT_3_TM=60.154
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=111
PRIMER_PAIR_PENALTY_4=0.4048
PRIMER_LEFT_4_PENALTY=0.102276
PRIMER_RIGHT_4_PENALTY=0.302565
PRIMER_LEFT_4_SEQUENCE=ACCATCGCCACCAGATATTAAC
PRIMER_RIGHT_4_SEQUENCE=GGAGGCTAAATCCTGGAAAAAC
PRIMER_LEFT_4=146,22
PRIMER_RIGHT_4=269,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=60.303
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=124
=
PRIMER_SEQUENCE_ID=SSR_06173 Ps_contig_mira-and-tgicl-ass_franssen_42221
SEQUENCE=GTTTAGTTAACCTCTATTTCCAATTAATGCATGCATGCAAATATAACACTACATCACAAACTGTTGCTTCTATATACACAACCAAATCCAATGAAGAAGAAATACAGAACTATTTCTTTCATGCTTTACCCTCAACCCTTGCATGGGTGAGTCCAACACATGCATCAACAAAATCCAAGTCTATAAAATCTGTAGAAAATATCTGAAATCTATCACCACACCCTTTAGAAAAGCACTGACTAATAAAGAGTCTTGCATATCCATGAATGCGTTCTGTCACGGGTTTCACGCACACAATCGGGTTATCAGGAACCGGTGTAACAGTATTCTCAACTCGATAGAAGTATTTTCTAGACACGACACGTTCCTGTTCGCTCAAATACTTCATATTACTTTCAAACTTTGTCAACGGCAAATCTGTGTTGATCCAATTATCCCTTAGATAGCCTGCACTCCAAAGACTACTACCCGCCTTTGGTTGTGTCGATTTTCTCGGCTTCCAAACGCATATAACTCTTTTTGGTAGCACCTCGGCAATAGTTTTATCAAAAACAGCATCGTCTTGGTGAATTAAGTACTCGCCTAGGTTTTCATCGAGATACTTGTCGAATTCAGGTGGATCATTATGTCCAAACTCGGTTCTAACCTCGAGGATTATGATTTCGGATTCGGTTTCTGATAAGAACTTTTTTACGTCTCTAATGACAA
TARGET=27,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06174 Ps_contig_mira-and-tgicl-ass_franssen_42222
SEQUENCE=GACGGGGAAACGAAAGGAAGACAAAAGAAAAGGAAAAGAAAAGAAGCCGCCAAAGAAAAAAGAAAAGACAAATACAAAAGAAGAAGCTAACACCACTCGTCCATCTTCCATAGAAAGTGAAATTTATTTGGTGTTTTGTGAATCAATCTTTCATCAAAGTTTTGTTCTTTAGTTATCCTCCTCCTCCTCCACATGGGTCGCCTTATTTTCTTTCTCATCATTCTAACCTTCTTTACATTATCTTTCCTCTCAGAGGTACAGTCAACATCGTTCAAGATTGTAAACAAGTGCCGTCACACAATATGGCCAGGATTACTCTCAGGCGCCACCTCGCCGCCGCTACCCACTACCGGATTCACCCTCAAGAGCGGAGAGTCAAGGACTGTCAACATCCCGAAATCTTGGTCGGGTCGGTTATGGGCACGG
TARGET=176,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3491
PRIMER_LEFT_PENALTY=0.183585
PRIMER_RIGHT_PENALTY=0.165541
PRIMER_LEFT_SEQUENCE=AAGAAGCTAACACCACTCGTCC
PRIMER_RIGHT_SEQUENCE=CGGCACTTGTTTACAATCTTGA
PRIMER_LEFT=80,22
PRIMER_RIGHT=292,22
PRIMER_LEFT_TM=59.816
PRIMER_RIGHT_TM=60.166
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=0.3613
PRIMER_LEFT_1_PENALTY=0.195770
PRIMER_RIGHT_1_PENALTY=0.165541
PRIMER_LEFT_1_SEQUENCE=AGCTAACACCACTCGTCCATCT
PRIMER_RIGHT_1_SEQUENCE=CGGCACTTGTTTACAATCTTGA
PRIMER_LEFT_1=84,22
PRIMER_RIGHT_1=292,22
PRIMER_LEFT_1_TM=60.196
PRIMER_RIGHT_1_TM=60.166
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=209
PRIMER_PAIR_PENALTY_2=0.3929
PRIMER_LEFT_2_PENALTY=0.183585
PRIMER_RIGHT_2_PENALTY=0.209343
PRIMER_LEFT_2_SEQUENCE=AAGAAGCTAACACCACTCGTCC
PRIMER_RIGHT_2_SEQUENCE=TAATCCTGGCCATATTGTGTGA
PRIMER_LEFT_2=80,22
PRIMER_RIGHT_2=314,22
PRIMER_LEFT_2_TM=59.816
PRIMER_RIGHT_2_TM=60.209
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=235
PRIMER_PAIR_PENALTY_3=0.4051
PRIMER_LEFT_3_PENALTY=0.195770
PRIMER_RIGHT_3_PENALTY=0.209343
PRIMER_LEFT_3_SEQUENCE=AGCTAACACCACTCGTCCATCT
PRIMER_RIGHT_3_SEQUENCE=TAATCCTGGCCATATTGTGTGA
PRIMER_LEFT_3=84,22
PRIMER_RIGHT_3=314,22
PRIMER_LEFT_3_TM=60.196
PRIMER_RIGHT_3_TM=60.209
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=231
PRIMER_PAIR_PENALTY_4=0.4466
PRIMER_LEFT_4_PENALTY=0.183585
PRIMER_RIGHT_4_PENALTY=0.263023
PRIMER_LEFT_4_SEQUENCE=AAGAAGCTAACACCACTCGTCC
PRIMER_RIGHT_4_SEQUENCE=TGAGAAAGAAAATAAGGCGACC
PRIMER_LEFT_4=80,22
PRIMER_RIGHT_4=216,22
PRIMER_LEFT_4_TM=59.816
PRIMER_RIGHT_4_TM=59.737
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=137
=
PRIMER_SEQUENCE_ID=SSR_06175 Ps_contig_mira-and-tgicl-ass_franssen_42228
SEQUENCE=AGAGTTCTAGGTTGTGAAATTTGTCTTTTTTTTTGTTGGAAAAAAATGGCTAATGAAGGAAACAAAAGCAATGATTTTTATGCGGTTTTGGGATTGAATAAAGAATGCACTGATTCAGAGCTAAGGAATGCTTATAAGAAACTTGCAATGAAATGGCATCCAGATCGTTGTTCAGCTTCAGGAAATGTGAAGTTTGTGGAAGAAGCTAAGAAGAAATTTCAGGCGATTCAAGAGGCCTATTCTGTTTTATCTGACGCAAATAAGAGGTTAATGTATGACGTTGGAGTTTACGACAATGATGATGATGAAAATGGTATGGGTGATTTTTTGAACGAAATGGTTACAATGATGAGCCAAACTACTTCAAATGAAAATGGAGAGGAGAGCTTTGAGGAGTTGCAGCAGTTGTTTAGTGATATGTTTCAAGCAGATATTGGATTGGATGGAAGCAGTTCTATGAATGCTTCTACTTCATCTACTTTCATGACTTATAATGAAAGTTCTAATTCTAATAAACGCAGTTCCAACCAGATGAATTATGGAATGGCGGGCGATTCGTCTAGCTTCGACGCTAATTATCAGAACTTCTGTTTTGGGACAGGTGAAGCCTCATCAAGAAGATGTGGCGGAGAAGGAAAAAGGGGGAATTCAAGAAAGAGGAGGTAGCAATAGACAAAGAAAAGGCAGAAAACAAAAAATCTCATATGGCCATGATGTTTCCTCTAATGACAATCATGGAATTTCTGCAAACTAGTGTGTCAACCTTAGTATTTTGGTCTTGAGAATTTCACAATCTTGACTCGCTACAACAAAAAAAAGGTTAGGAGCAAGAACATATAGTTTATGTTAGTACTATGAAGTCGTAGGGAGGATGTCACAAATGTCACCCTTCAAGCCATTGGACACTTATGTTAAGAGTTTGAGTTTAAATTTAATTGACAAGTCATTTTGGTATTTTTCTATTTATGCATATTTGTATCATTTCATTTCAAGAATTAATTTAAATTACAAAAGTAGTAATTGTTTTAGTTCATTTGTACT
TARGET=296,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1411
PRIMER_LEFT_PENALTY=0.008257
PRIMER_RIGHT_PENALTY=0.132857
PRIMER_LEFT_SEQUENCE=CAGGAAATGTGAAGTTTGTGGA
PRIMER_RIGHT_SEQUENCE=GTTTGGCTCATCATTGTAACCA
PRIMER_LEFT=178,22
PRIMER_RIGHT=358,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.867
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=181
PRIMER_PAIR_PENALTY_1=0.1411
PRIMER_LEFT_1_PENALTY=0.008257
PRIMER_RIGHT_1_PENALTY=0.132857
PRIMER_LEFT_1_SEQUENCE=TCAGGAAATGTGAAGTTTGTGG
PRIMER_RIGHT_1_SEQUENCE=GTTTGGCTCATCATTGTAACCA
PRIMER_LEFT_1=177,22
PRIMER_RIGHT_1=358,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=0.1429
PRIMER_LEFT_2_PENALTY=0.010077
PRIMER_RIGHT_2_PENALTY=0.132857
PRIMER_LEFT_2_SEQUENCE=GCTAAGAAGAAATTTCAGGCGA
PRIMER_RIGHT_2_SEQUENCE=GTTTGGCTCATCATTGTAACCA
PRIMER_LEFT_2=204,22
PRIMER_RIGHT_2=358,22
PRIMER_LEFT_2_TM=59.990
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=11.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=155
PRIMER_PAIR_PENALTY_3=0.3007
PRIMER_LEFT_3_PENALTY=0.008257
PRIMER_RIGHT_3_PENALTY=0.292414
PRIMER_LEFT_3_SEQUENCE=TCAGGAAATGTGAAGTTTGTGG
PRIMER_RIGHT_3_SEQUENCE=TCATAGAACTGCTTCCATCCAA
PRIMER_LEFT_3=177,22
PRIMER_RIGHT_3=459,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.708
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.3007
PRIMER_LEFT_4_PENALTY=0.008257
PRIMER_RIGHT_4_PENALTY=0.292414
PRIMER_LEFT_4_SEQUENCE=TCAGGAAATGTGAAGTTTGTGG
PRIMER_RIGHT_4_SEQUENCE=TTCATAGAACTGCTTCCATCCA
PRIMER_LEFT_4=177,22
PRIMER_RIGHT_4=460,22
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=59.708
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=284
=
PRIMER_SEQUENCE_ID=SSR_06176 Ps_contig_mira-and-tgicl-ass_franssen_42235
SEQUENCE=CGTCTAGAAGATTGACAATATTTTTATATATATATATTTTTGTAAATCTTGATATCCGACCTTGCGACCGACTAATCCAAGAGGATCAATTTCACCATTCACAAGCGGGCAGCCATAAACTATGATGCTTTCTATCTTGTTCATGTTGATTTCATGATAATCCAAAACCAAATACACAATAAAAACAATAAATAAAACTCNTCATCCATCACATATA
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06177 Ps_contig_mira-and-tgicl-ass_franssen_42236
SEQUENCE=GAGAAGAAGTTGTCCTGGAATTATACTTGCATTGCCAATATTGGGATTGGTGGTTGCTAAGTTGGTGTCAAATTTTGAGATGAAGGGTTTGAATGGGATGAAGATTGATGTTAGTGAAAAAGGTGGACAATTTAGTTTGCATATTGCTAATCATTCAACTGTCTTGTTTCATCCAATTGATTAAAGCATGATGATGATGATGTATCAACTCAAGATGCAAAATTGTTTGTTCTTTACTTAAATTAAGTTTTGGTTTTGTTTTTTCTTTTTCTTTATTTTTGTTATTTTTTTTGTA
TARGET=188,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06178 Ps_contig_mira-and-tgicl-ass_franssen_42239
SEQUENCE=TATGCTTCAAAATCTCAATTTTTCATCACTTCCGTTCCAAGCATTTCAGCCTTCCAAAATGCTTCATTTGAAGCACTCAGAAATAACAAACTTGTGTGGTAATGTATTGGGAAAGAAGTGATATTGCTTAACACAGAGTCGGCATTGTCGCCCTACAATGAAAAATGTTTGCAAGGAGATTGTAATGTCAAAGTCATCTTCAAGGGGTTGATTTTGTACATAACGTCATTTAAGTATAGCTAAGAGTCAATGTTTGTTGTATCATCGAAAGGGTCTCCTAAAAGGTCTAAAGCCGAGATGGAAGCTCTTAAGGGAGCTAAAGGGGTGACCAAGTTTGTGGAAGTGCCTGGTGCTCTTCTTCCACAAGAGGAGTACCCTGCTGAAGTCGCGGAAGAGCTTTATCAATTCTTGCAACAGTATTTTGGATCTGTCGCTTGAAGTTCACACGTCGTGTGACACTTCTGTATGATGGCGGTGAAGGTTTCACGGGCATAAACACTAATTGAAGTAAGAAAGAAAGACAGAAATGTGACCAAGGGTATAGTGTGGTTTATTTGTAATTTTTTTAAAGAAACTTTGGTATGTTATGAGTTTTATACACATGTTAAAATCATTTCCGAAAGGAATGTTGTATTTGAAACATAACTTCTAGTAGTAAANTTTAA
TARGET=510,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2607
PRIMER_LEFT_PENALTY=0.034674
PRIMER_RIGHT_PENALTY=0.225996
PRIMER_LEFT_SEQUENCE=ATCGAAAGGGTCTCCTAAAAGG
PRIMER_RIGHT_SEQUENCE=CCACACTATACCCTTGGTCACA
PRIMER_LEFT=263,22
PRIMER_RIGHT=548,22
PRIMER_LEFT_TM=59.965
PRIMER_RIGHT_TM=59.774
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=286
PRIMER_PAIR_PENALTY_1=0.2644
PRIMER_LEFT_1_PENALTY=0.038361
PRIMER_RIGHT_1_PENALTY=0.225996
PRIMER_LEFT_1_SEQUENCE=TGTATCATCGAAAGGGTCTCCT
PRIMER_RIGHT_1_SEQUENCE=CCACACTATACCCTTGGTCACA
PRIMER_LEFT_1=257,22
PRIMER_RIGHT_1=548,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=59.774
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.2943
PRIMER_LEFT_2_PENALTY=0.068302
PRIMER_RIGHT_2_PENALTY=0.225996
PRIMER_LEFT_2_SEQUENCE=TCATCGAAAGGGTCTCCTAAAA
PRIMER_RIGHT_2_SEQUENCE=CCACACTATACCCTTGGTCACA
PRIMER_LEFT_2=261,22
PRIMER_RIGHT_2=548,22
PRIMER_LEFT_2_TM=60.068
PRIMER_RIGHT_2_TM=59.774
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=288
PRIMER_PAIR_PENALTY_3=0.5279
PRIMER_LEFT_3_PENALTY=0.301926
PRIMER_RIGHT_3_PENALTY=0.225996
PRIMER_LEFT_3_SEQUENCE=ACAAGAGGAGTACCCTGCTGAA
PRIMER_RIGHT_3_SEQUENCE=CCACACTATACCCTTGGTCACA
PRIMER_LEFT_3=362,22
PRIMER_RIGHT_3=548,22
PRIMER_LEFT_3_TM=60.302
PRIMER_RIGHT_3_TM=59.774
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=187
PRIMER_PAIR_PENALTY_4=0.5456
PRIMER_LEFT_4_PENALTY=0.319597
PRIMER_RIGHT_4_PENALTY=0.225996
PRIMER_LEFT_4_SEQUENCE=TTGTATCATCGAAAGGGTCTCC
PRIMER_RIGHT_4_SEQUENCE=CCACACTATACCCTTGGTCACA
PRIMER_LEFT_4=256,22
PRIMER_RIGHT_4=548,22
PRIMER_LEFT_4_TM=60.320
PRIMER_RIGHT_4_TM=59.774
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=293
=
PRIMER_SEQUENCE_ID=SSR_06179 Ps_contig_mira-and-tgicl-ass_franssen_42243
SEQUENCE=AAGRAAAGAAAAGAAAAGAGAAGAGAATAGAACCATGCGCGCGATTCCAGTCTCCACAGAAGCATTCCTATTCCCTACACCATCACCCACTCCCATTTCCATTACCCATTCCTCCACTCTTTTACACCCTCATCCTTTTCATTACAACAAAACCCTTTCCACCACACCATGCTCCTTCAACTCTAAAACCACTCCATTCTTCTACCCAGCTTCCAAATCCAACAACCCAATCACTCTGGACGACGATGCCGACGACGACGATTCTGAGAATTCCTTGCTCACGGCGGATACCCAAGCTCAAGAGCCGGACTCCTTGGTTGAGGACGGTAGTTTTCATAGAGGTGACCAAGCTTGAGAAGAACTCACGCAGAATCGAGTCGAGGATTTCAATCCACGCTTCACTTGATTCCGTTTGGAGTATCTTGACTGATTATGAGAGGTTGGCGGATTTTATTCCTGGCCTTGCTCTTAGTAAATTGATCCAAAAAGCTCCCAATTTTGCTAGACTTTTGCAGATTGGAGAGCAGAACTTGGCATTTGGGCTAAAATTTGATGCTAAAGGAGTTATAGATTGTTTCGAGAAAGACTTGGAGACTCTTCCATCTGGTATGAAGCGTGACATAGACTTCAAGATGATTGAAGGGGATTTTCAACTCTTTGAAGGAAAATGGTCTATTTCACAGCTTTTCAGCAGTGGAAGTGATGAGTCTCCAATACGGGAGATTAGCACGACACTTACATACATTGTTGACGTTAAGCCGAAGTTGTGGTTGCCAGTTCGGCTCATAGAGGGTAGACTTTGTAAAGAGATAAAAAAGAACCTTATATCCATAAGGGGCGAAGCTCAGAAAGCTACTGATAGAACTCTGGATGTAAATCAATTCGGATGAGTTATGTAAATATTATCATAAGAAAAAATGAATACAAGTCAAATTCATTGCGTCAATTCTCACATTGTAAAGTTTTCAAGGTTGGCCATATTCTGTTCCTAAATTATGTT
TARGET=5,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06180 Ps_contig_mira-and-tgicl-ass_franssen_42243
SEQUENCE=AAGRAAAGAAAAGAAAAGAGAAGAGAATAGAACCATGCGCGCGATTCCAGTCTCCACAGAAGCATTCCTATTCCCTACACCATCACCCACTCCCATTTCCATTACCCATTCCTCCACTCTTTTACACCCTCATCCTTTTCATTACAACAAAACCCTTTCCACCACACCATGCTCCTTCAACTCTAAAACCACTCCATTCTTCTACCCAGCTTCCAAATCCAACAACCCAATCACTCTGGACGACGATGCCGACGACGACGATTCTGAGAATTCCTTGCTCACGGCGGATACCCAAGCTCAAGAGCCGGACTCCTTGGTTGAGGACGGTAGTTTTCATAGAGGTGACCAAGCTTGAGAAGAACTCACGCAGAATCGAGTCGAGGATTTCAATCCACGCTTCACTTGATTCCGTTTGGAGTATCTTGACTGATTATGAGAGGTTGGCGGATTTTATTCCTGGCCTTGCTCTTAGTAAATTGATCCAAAAAGCTCCCAATTTTGCTAGACTTTTGCAGATTGGAGAGCAGAACTTGGCATTTGGGCTAAAATTTGATGCTAAAGGAGTTATAGATTGTTTCGAGAAAGACTTGGAGACTCTTCCATCTGGTATGAAGCGTGACATAGACTTCAAGATGATTGAAGGGGATTTTCAACTCTTTGAAGGAAAATGGTCTATTTCACAGCTTTTCAGCAGTGGAAGTGATGAGTCTCCAATACGGGAGATTAGCACGACACTTACATACATTGTTGACGTTAAGCCGAAGTTGTGGTTGCCAGTTCGGCTCATAGAGGGTAGACTTTGTAAAGAGATAAAAAAGAACCTTATATCCATAAGGGGCGAAGCTCAGAAAGCTACTGATAGAACTCTGGATGTAAATCAATTCGGATGAGTTATGTAAATATTATCATAAGAAAAAATGAATACAAGTCAAATTCATTGCGTCAATTCTCACATTGTAAAGTTTTCAAGGTTGGCCATATTCTGTTCCTAAATTATGTT
TARGET=250,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06181 Ps_contig_mira-and-tgicl-ass_franssen_42250
SEQUENCE=AAATTCTAATTGTTGRGGAAAATNAACTACTAGAAGGGGCTTAGTAGTTTGTGATCGTGATCGGAAGATGGAGGGAGGAGGAGGATACGCGTGGGCAGTATCTGCTGGATTCAACGCCGCTCTTGCAGCCATCTTCGCCAAGCTTACCTTTCACATCTTGATTAAATATGGCCTGGTGGTATTGTTCAATGTAACCATGTGGGGTTGTTATGTCAACAGCCTTAAAGCTCTTTCATCTCTTCAAGCTACTGTCACCAATTTCGCTACCAATTTCATCACTTCTGGTTTAGCTGGTTTCTTCTTCTTTCACGAATCACTCTCTTTCCAGGTGCCCTACTCATAATAATTGGTGTAATAATACTTAGCAACTCGAATATTGAAAAGAAGGTTAGCTCTGATTAGATGATGTGTTCCGCATGGTTCCGCTCAAGCTTTTGTAAAAGGAGGAAGCTAGCATCTGCAACTGTATGAACAATAATTTGTAACATCTGAAATAGTATAGTTCGATCATCTGCTGGGTAATTCACTCCTCACATGAAACAGGCTGAACAAGTTTGCTAAATTAACAGCTTGCTCGATGTTTTATTGACTTTATTTATGTGGTTCTGTGGTGTAAGTTGGTTTGAGATGAATGCCAATGTTTTAGAGGCAGATCTTTGATGTTATTATATTTGTATGATGCTGGTCATGTTACATTGTTCTTCTTTTGGTTTCTTTTCTNAYAATTGAATTGGACAGAATTGGTGCTATTTGTAAGAGACTAACTTTAAAAAGATAAACG
TARGET=74,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06182 Ps_contig_mira-and-tgicl-ass_franssen_42257
SEQUENCE=ACCATAACAACGTAGTGTATTTATAAATGAGCAAACATACTTAATTACAATGCAAACATCATACACTAATCAACCAGAAAATCATGGTTCAAGACCTTATACATCAAAACATACATAAACAACCACAACAACAACACCCTATCTCCACCTAATTATTCCTTAATTAACCTTTTGGCTAGAATAATAAGCCTTAAAAACCCTAGGAAGATAAACAGGAACCAAAAACCCAAACAAATTAAAACACCAAAAAGCCATATTCGCCACAGCAAGTGCTCTTCCAGCATAAATCCTTCTATAAGAGCCACTATGTTCTTCACCAACCTTGTAAATCTCATCTCTCAACCAATCCACAATGGTAAAAACCCTTCTCGCATTATACAAAACAGGAACAAAAGCTCTTATCGGAGACGAAAATCCATCAGAAAAAGCCACACCTTCCATAAAAACTTGACTAGCCAAAAGAAAAACATGTGGCGTAGCTGCGGCTATACCTTTCTTATCACCTTCGAATACTCCATGTAGAATATACGCTATAGGAGCATAGAGTCCAACGATTGCCCCTAACAGAACATAGAGTTTAAGGATTTTGTTCTGTCGGCTGAAAATTAGGTTAGCTCGTTTGAAGGGTGAAGAGATGGAAAAG
TARGET=125,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5350
PRIMER_LEFT_PENALTY=1.446387
PRIMER_RIGHT_PENALTY=0.088662
PRIMER_LEFT_SEQUENCE=CCAGAAAATCATGGTTCAAGAC
PRIMER_RIGHT_SEQUENCE=GAAGGGTTTTTACCATTGTGGA
PRIMER_LEFT=73,22
PRIMER_RIGHT=367,22
PRIMER_LEFT_TM=58.554
PRIMER_RIGHT_TM=60.089
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=295
PRIMER_PAIR_PENALTY_1=1.5350
PRIMER_LEFT_1_PENALTY=1.446387
PRIMER_RIGHT_1_PENALTY=0.088662
PRIMER_LEFT_1_SEQUENCE=CAGAAAATCATGGTTCAAGACC
PRIMER_RIGHT_1_SEQUENCE=GAAGGGTTTTTACCATTGTGGA
PRIMER_LEFT_1=74,22
PRIMER_RIGHT_1=367,22
PRIMER_LEFT_1_TM=58.554
PRIMER_RIGHT_1_TM=60.089
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=294
PRIMER_PAIR_PENALTY_2=1.5575
PRIMER_LEFT_2_PENALTY=1.446387
PRIMER_RIGHT_2_PENALTY=0.111077
PRIMER_LEFT_2_SEQUENCE=CCAGAAAATCATGGTTCAAGAC
PRIMER_RIGHT_2_SEQUENCE=TGTGGATTGGTTGAGAGATGAG
PRIMER_LEFT_2=73,22
PRIMER_RIGHT_2=351,22
PRIMER_LEFT_2_TM=58.554
PRIMER_RIGHT_2_TM=60.111
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=1.5575
PRIMER_LEFT_3_PENALTY=1.446387
PRIMER_RIGHT_3_PENALTY=0.111077
PRIMER_LEFT_3_SEQUENCE=CAGAAAATCATGGTTCAAGACC
PRIMER_RIGHT_3_SEQUENCE=TGTGGATTGGTTGAGAGATGAG
PRIMER_LEFT_3=74,22
PRIMER_RIGHT_3=351,22
PRIMER_LEFT_3_TM=58.554
PRIMER_RIGHT_3_TM=60.111
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=278
PRIMER_PAIR_PENALTY_4=1.6704
PRIMER_LEFT_4_PENALTY=1.446387
PRIMER_RIGHT_4_PENALTY=0.224014
PRIMER_LEFT_4_SEQUENCE=CCAGAAAATCATGGTTCAAGAC
PRIMER_RIGHT_4_SEQUENCE=AAGGATTTATGCTGGAAGAGCA
PRIMER_LEFT_4=73,22
PRIMER_RIGHT_4=291,22
PRIMER_LEFT_4_TM=58.554
PRIMER_RIGHT_4_TM=60.224
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_06183 Ps_contig_mira-and-tgicl-ass_franssen_42260
SEQUENCE=AGGAATTTAGATTACGAACTTGTTTGTGGTGGTGAGTAACACGTAATGCATTATTGTATTTGTCTACATGTATAAATAAATTGAGAGCGTAGTAATTCGGGGGAGAACATTAATAGATGAGCGATAGAAAGAAAATGGGAAAGGCGAAAGAAGCGGTTGTGGTGGCGGGTGCCCTAGCATTTGTCTGGCTCGCTATTGAACTCGCTTTCAAACCCTTCCTTTCTCAGACCCGTGACTCCATCGACAAATCCGACCCGACCCGGGATCCTGACGATGCTCCTCCTCCTCCTCCTCCTGAAACTGACGCCGGAGATGCCGACAAAGACGACTGATGATTTGAGCGCGGTTGTTACTATTCCTTTCTTGCATTCTGTATTTCGTTAAATTGTTAAGTGAAATAACATTAACTATGTTCTTTGTTGTGTTTTCTGTTCTGATCTGATTTCTGGAATACTGTAATTTTAGGATTAGGATTGAATGCCAGGTTGTTCACTTCATACGTTGGACAATTGGTTTGTTAATTTGGTGTTTGTCTCTTTAGCTATTGCAAATGCAATCGTGTTGTGAAAATAACAAAGCTTCTGCAATCATTTTTCACATTATGTTTCGNNAAAATCAACTAAAATACAAAAACACAATGTGAACTTCTGGGTTGAGCTAGGATTTGAAGCAAATCTCAGATATAAAATGTATTCTTGGACTATTTTTAGATTAGAGACGATTAATTTG
TARGET=277,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2470
PRIMER_LEFT_PENALTY=0.197549
PRIMER_RIGHT_PENALTY=0.049498
PRIMER_LEFT_SEQUENCE=TTCGGGGGAGAACATTAATAGA
PRIMER_RIGHT_SEQUENCE=ATAGTAACAACCGCGCTCAAAT
PRIMER_LEFT=95,22
PRIMER_RIGHT=355,22
PRIMER_LEFT_TM=59.802
PRIMER_RIGHT_TM=60.049
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=0.3018
PRIMER_LEFT_1_PENALTY=0.264444
PRIMER_RIGHT_1_PENALTY=0.037339
PRIMER_LEFT_1_SEQUENCE=CTTTCAAACCCTTCCTTTCTCA
PRIMER_RIGHT_1_SEQUENCE=GTATGAAGTGAACAACCTGGCA
PRIMER_LEFT_1=204,22
PRIMER_RIGHT_1=499,22
PRIMER_LEFT_1_TM=59.736
PRIMER_RIGHT_1_TM=60.037
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=0.3018
PRIMER_LEFT_2_PENALTY=0.264444
PRIMER_RIGHT_2_PENALTY=0.037339
PRIMER_LEFT_2_SEQUENCE=TTTCAAACCCTTCCTTTCTCAG
PRIMER_RIGHT_2_SEQUENCE=GTATGAAGTGAACAACCTGGCA
PRIMER_LEFT_2=205,22
PRIMER_RIGHT_2=499,22
PRIMER_LEFT_2_TM=59.736
PRIMER_RIGHT_2_TM=60.037
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.3081
PRIMER_LEFT_3_PENALTY=0.270774
PRIMER_RIGHT_3_PENALTY=0.037339
PRIMER_LEFT_3_SEQUENCE=TCAAACCCTTCCTTTCTCAGAC
PRIMER_RIGHT_3_SEQUENCE=GTATGAAGTGAACAACCTGGCA
PRIMER_LEFT_3=207,22
PRIMER_RIGHT_3=499,22
PRIMER_LEFT_3_TM=59.729
PRIMER_RIGHT_3_TM=60.037
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=10.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=293
PRIMER_PAIR_PENALTY_4=0.3139
PRIMER_LEFT_4_PENALTY=0.264444
PRIMER_RIGHT_4_PENALTY=0.049498
PRIMER_LEFT_4_SEQUENCE=CTTTCAAACCCTTCCTTTCTCA
PRIMER_RIGHT_4_SEQUENCE=ATAGTAACAACCGCGCTCAAAT
PRIMER_LEFT_4=204,22
PRIMER_RIGHT_4=355,22
PRIMER_LEFT_4_TM=59.736
PRIMER_RIGHT_4_TM=60.049
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_06184 Ps_contig_mira-and-tgicl-ass_franssen_42263
SEQUENCE=AAAGAAAAAGTTCAAGGGCTAAAAACCATGAACATCATATATACATATGATGTAGATAAAGGTTCAACTAATTAAAAAAATTAAGTGAATCTTATACTCATAATTATACAAAATGGAAAAAGTGAGTAGGTTCTATACCCCCACTAAAAAGGAACCACATTCTCTTCCTTTTTGCACATAGAAGGAACAAAACAAAAGCAAAAAGAACAGAAGATTAAGGTGGGGTTTGTTCCTGGATGATTCTTTCTCAAGTGAGATGAGGAAATTACATAAAACACCCATCCTTCATCATGTAGTGGCACAAAATCTCACCCACACTATAAACCACCCATAACTTTTTGCTTAAAAGGTTTATTATTTTTAGTCCCTTAAAGTTACTAGCCATAAAGTAAAAACAAGGGATAGTAACAGAACATAACTACATTTTTTTAATTGAACCACTCCATTTGGGTCAGTGAAGCCACTTCCATTGCCGGTTGAAGATGTTGGACTCATCCCAAACACGTTAGGACCACCTGTGACTGTGCCTGTTCCTGTGCCGGTGCCTGTACCTGTTCCTGTGCCTGTTGGTGTGCCTGTTCCTGTGCCTGTACCTGTTCCTGTTCCTGTTCCTGGTGTTGTACCTACAGGTGTGGTTGGAGTTATGGTGGAAGGTGGTGCTGTGCCTACAGGTGTATTGGTTCCTGGTGTTGTGCCTGTACCTCCTCCTGCATTGCCACTTGAAGGAAAAACACATGTAGTTGTTGAAGTAGTAGGTGGAGTTGGACTGGTAACAGCAGAACCAGAAAAATCACAGCTACCTTGAGTTTGACCCTTTTTCTGGAAATAGCTATTAACAGCATAATTGCAGTGATCTTTCACAGTATTGGGTTGAAAACATGATCCAGATTGTAGAATAGGTGAACAATCAGCTCCAGCACCACAAGCATAATCTATTGCCCTTTGAAGATTTTGATCTCCAACACCATCTCTACACACACAATAAAGAGCACCACTTGAATATCCAGTTAGAGAAAGGAAAAGCACAAAATAAGGCAGAAGAACCATGATTAAGGAAGGTTATGATGTTGTTAGAGAAATCTATTTGATGGAGAAATTGGATCAAGTTCAGAAGGATACTCCAATATGTGGCAGAGAGAGAGAAAGAGAGAGAGAAGAATGAAATTTCTGGACAAGAGATATGTGTGTTGTACCGTTTTCTAGGACTGTTATGTTTGTAATAACGTTATTNTTTGTA
TARGET=1133,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0107
PRIMER_LEFT_PENALTY=0.127927
PRIMER_RIGHT_PENALTY=1.882731
PRIMER_LEFT_SEQUENCE=GGAAAAGCACAAAATAAGGCAG
PRIMER_RIGHT_SEQUENCE=CCTAGAAAACGGTACAACACACA
PRIMER_LEFT=1016,22
PRIMER_RIGHT=1203,23
PRIMER_LEFT_TM=60.128
PRIMER_RIGHT_TM=59.117
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=2.0346
PRIMER_LEFT_1_PENALTY=0.151911
PRIMER_RIGHT_1_PENALTY=1.882731
PRIMER_LEFT_1_SEQUENCE=CCAGCACCACAAGCATAATCTA
PRIMER_RIGHT_1_SEQUENCE=CCTAGAAAACGGTACAACACACA
PRIMER_LEFT_1=913,22
PRIMER_RIGHT_1=1203,23
PRIMER_LEFT_1_TM=60.152
PRIMER_RIGHT_1_TM=59.117
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=291
PRIMER_PAIR_PENALTY_2=2.5742
PRIMER_LEFT_2_PENALTY=0.691450
PRIMER_RIGHT_2_PENALTY=1.882731
PRIMER_LEFT_2_SEQUENCE=CCACAAGCATAATCTATTGCCC
PRIMER_RIGHT_2_SEQUENCE=CCTAGAAAACGGTACAACACACA
PRIMER_LEFT_2=919,22
PRIMER_RIGHT_2=1203,23
PRIMER_LEFT_2_TM=60.691
PRIMER_RIGHT_2_TM=59.117
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=2.6781
PRIMER_LEFT_3_PENALTY=0.795397
PRIMER_RIGHT_3_PENALTY=1.882731
PRIMER_LEFT_3_SEQUENCE=GCCCTTTGAAGATTTTGATCTC
PRIMER_RIGHT_3_SEQUENCE=CCTAGAAAACGGTACAACACACA
PRIMER_LEFT_3=937,22
PRIMER_RIGHT_3=1203,23
PRIMER_LEFT_3_TM=59.205
PRIMER_RIGHT_3_TM=59.117
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=2.7208
PRIMER_LEFT_4_PENALTY=0.127927
PRIMER_RIGHT_4_PENALTY=2.592902
PRIMER_LEFT_4_SEQUENCE=GGAAAAGCACAAAATAAGGCAG
PRIMER_RIGHT_4_SEQUENCE=CCTAGAAAACGGTACAACACACAT
PRIMER_LEFT_4=1016,22
PRIMER_RIGHT_4=1203,24
PRIMER_LEFT_4_TM=60.128
PRIMER_RIGHT_4_TM=59.407
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_06185 Ps_contig_mira-and-tgicl-ass_franssen_42263
SEQUENCE=AAAGAAAAAGTTCAAGGGCTAAAAACCATGAACATCATATATACATATGATGTAGATAAAGGTTCAACTAATTAAAAAAATTAAGTGAATCTTATACTCATAATTATACAAAATGGAAAAAGTGAGTAGGTTCTATACCCCCACTAAAAAGGAACCACATTCTCTTCCTTTTTGCACATAGAAGGAACAAAACAAAAGCAAAAAGAACAGAAGATTAAGGTGGGGTTTGTTCCTGGATGATTCTTTCTCAAGTGAGATGAGGAAATTACATAAAACACCCATCCTTCATCATGTAGTGGCACAAAATCTCACCCACACTATAAACCACCCATAACTTTTTGCTTAAAAGGTTTATTATTTTTAGTCCCTTAAAGTTACTAGCCATAAAGTAAAAACAAGGGATAGTAACAGAACATAACTACATTTTTTTAATTGAACCACTCCATTTGGGTCAGTGAAGCCACTTCCATTGCCGGTTGAAGATGTTGGACTCATCCCAAACACGTTAGGACCACCTGTGACTGTGCCTGTTCCTGTGCCGGTGCCTGTACCTGTTCCTGTGCCTGTTGGTGTGCCTGTTCCTGTGCCTGTACCTGTTCCTGTTCCTGTTCCTGGTGTTGTACCTACAGGTGTGGTTGGAGTTATGGTGGAAGGTGGTGCTGTGCCTACAGGTGTATTGGTTCCTGGTGTTGTGCCTGTACCTCCTCCTGCATTGCCACTTGAAGGAAAAACACATGTAGTTGTTGAAGTAGTAGGTGGAGTTGGACTGGTAACAGCAGAACCAGAAAAATCACAGCTACCTTGAGTTTGACCCTTTTTCTGGAAATAGCTATTAACAGCATAATTGCAGTGATCTTTCACAGTATTGGGTTGAAAACATGATCCAGATTGTAGAATAGGTGAACAATCAGCTCCAGCACCACAAGCATAATCTATTGCCCTTTGAAGATTTTGATCTCCAACACCATCTCTACACACACAATAAAGAGCACCACTTGAATATCCAGTTAGAGAAAGGAAAAGCACAAAATAAGGCAGAAGAACCATGATTAAGGAAGGTTATGATGTTGTTAGAGAAATCTATTTGATGGAGAAATTGGATCAAGTTCAGAAGGATACTCCAATATGTGGCAGAGAGAGAGAAAGAGAGAGAGAAGAATGAAATTTCTGGACAAGAGATATGTGTGTTGTACCGTTTTCTAGGACTGTTATGTTTGTAATAACGTTATTNTTTGTA
TARGET=1145,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.0107
PRIMER_LEFT_PENALTY=0.127927
PRIMER_RIGHT_PENALTY=1.882731
PRIMER_LEFT_SEQUENCE=GGAAAAGCACAAAATAAGGCAG
PRIMER_RIGHT_SEQUENCE=CCTAGAAAACGGTACAACACACA
PRIMER_LEFT=1016,22
PRIMER_RIGHT=1203,23
PRIMER_LEFT_TM=60.128
PRIMER_RIGHT_TM=59.117
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=188
PRIMER_PAIR_PENALTY_1=2.0346
PRIMER_LEFT_1_PENALTY=0.151911
PRIMER_RIGHT_1_PENALTY=1.882731
PRIMER_LEFT_1_SEQUENCE=CCAGCACCACAAGCATAATCTA
PRIMER_RIGHT_1_SEQUENCE=CCTAGAAAACGGTACAACACACA
PRIMER_LEFT_1=913,22
PRIMER_RIGHT_1=1203,23
PRIMER_LEFT_1_TM=60.152
PRIMER_RIGHT_1_TM=59.117
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=291
PRIMER_PAIR_PENALTY_2=2.5742
PRIMER_LEFT_2_PENALTY=0.691450
PRIMER_RIGHT_2_PENALTY=1.882731
PRIMER_LEFT_2_SEQUENCE=CCACAAGCATAATCTATTGCCC
PRIMER_RIGHT_2_SEQUENCE=CCTAGAAAACGGTACAACACACA
PRIMER_LEFT_2=919,22
PRIMER_RIGHT_2=1203,23
PRIMER_LEFT_2_TM=60.691
PRIMER_RIGHT_2_TM=59.117
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=43.478
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=11.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=2.6781
PRIMER_LEFT_3_PENALTY=0.795397
PRIMER_RIGHT_3_PENALTY=1.882731
PRIMER_LEFT_3_SEQUENCE=GCCCTTTGAAGATTTTGATCTC
PRIMER_RIGHT_3_SEQUENCE=CCTAGAAAACGGTACAACACACA
PRIMER_LEFT_3=937,22
PRIMER_RIGHT_3=1203,23
PRIMER_LEFT_3_TM=59.205
PRIMER_RIGHT_3_TM=59.117
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=43.478
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=267
PRIMER_PAIR_PENALTY_4=2.7208
PRIMER_LEFT_4_PENALTY=0.127927
PRIMER_RIGHT_4_PENALTY=2.592902
PRIMER_LEFT_4_SEQUENCE=GGAAAAGCACAAAATAAGGCAG
PRIMER_RIGHT_4_SEQUENCE=CCTAGAAAACGGTACAACACACAT
PRIMER_LEFT_4=1016,22
PRIMER_RIGHT_4=1203,24
PRIMER_LEFT_4_TM=60.128
PRIMER_RIGHT_4_TM=59.407
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=188
=
PRIMER_SEQUENCE_ID=SSR_06186 Ps_contig_mira-and-tgicl-ass_franssen_42266
SEQUENCE=AAGCAGTGGCATCAACGCAGAGTTTACGTTCGGGGGGAAGAAGAAGAGGATTAACGTTTGAACCTTCACAGACCCAGAAATTCTCCAAACTCCATTCCAATGGCTTTCACTTCTTCTCTCACTTCCCATTTTCTCTCTCTCCCAAACAAAAACCCATCCCTAACCCCTTTTCAATCTCAACCCTTCTTTTTCCCTACACTCCACCCAAAACCACTCTTATCATCACCTACTTCAATCTCAACCGTTGCTTCAGCGTCATCATCGAAAAACCCTTCCAGTAACAATAACCCTAAACAATCTAGACAAGATGAAGTTGTTGAGGTTGAAGTAGAAGAGGAACTTCCGTGGATTCAAGAGAAAGCTCTCGACCTCGTTGAGTTCACCGGTTCTGTTACCCAAGCGATTCCCGGTCCGAGAGTGGGTCCCACTTCATTGCCGTGGATTCTCGCCGTTCCTCTTGGTTATGCTGGTCTTACTTTCGTTATTGCGTTTGTTAAAACCGTTAGGAAATTCAGTTCTCCCAAAGCACAACGCAGGAAATTGGTTGGTAAAAATGCGTTGTTGTGTAAGTCTGTTGATGATTTGTTACAAAGAGGACGAGATGAAGTTAAGGTTGATGATCTCAAAGGAATCGAAAATAAGACAGGTTTTGTCTTGGAAGAAATTTTGCGCAAATACATCCGATATGCTCTGAACGAGAAACCATTTAACCCTGATGTTGTAGCTGATTTAATTCAATTAAGGAGAGCTTCTGGATTAAAGGACTCACAAGTTGCTGAGATTCTAAATGAAATTTCACGAAGAATTGTTAGAGACAAAGGGCCTATTGTAATGAATAAATCAGGTTATACTGAAAAGGGTTTCAAGAGAAAACTAGCTGTTCAAGCCCTTTTTGGAAAGGTCTTCTATCTATCTGAACTGCCAGAGTTTTGTTCAAGGGATAGCTCCTTATTTGTTAAGGAAATCTTCGGAGTAACAGATGAAGATGCGGATCAACTCAGATTACACACAACTTCAGAAGCCGGCAACCTAGATTCGCTTGAGAAGATGGTTGATAGTTCAGATTCAGAAGATTCTAGTGAGGTTTCATAATGCTTGATGCAAATATACCCCTAGGTTTTGAATTCACAAAAGTTGAGGAATTGGTTCTGCAAACAATCTTCTCATGACCCATGTGCTGCAAATTCTAGATTACCATCGTCATCTCCTATCCAAAAATGGAAGTAAAAATATCCAATGTGTTGGGCTAAGGTTTTTTTTCCTTAATTTCTTTTTAGTGGTACAACTATTGTATACTTTCAGCTTTTTCCGGATTAAATTAAGAGCTTGACATTTTCATCAGTGTCCTATAGGTCAGTCTTTTTTTTTGTTAGAACCAGATATTGTGGAGAAATTTAATTAACAAGTGTCATAGAGATATTTTTGGTATATATGTAGAATGATTTTAAGAT
TARGET=132,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0497
PRIMER_LEFT_PENALTY=0.023556
PRIMER_RIGHT_PENALTY=0.026163
PRIMER_LEFT_SEQUENCE=ACAGACCCAGAAATTCTCCAAA
PRIMER_RIGHT_SEQUENCE=TGTAGGGAAAAAGAAGGGTTGA
PRIMER_LEFT=67,22
PRIMER_RIGHT=197,22
PRIMER_LEFT_TM=59.976
PRIMER_RIGHT_TM=59.974
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=131
PRIMER_PAIR_PENALTY_1=0.0497
PRIMER_LEFT_1_PENALTY=0.023556
PRIMER_RIGHT_1_PENALTY=0.026163
PRIMER_LEFT_1_SEQUENCE=CAGACCCAGAAATTCTCCAAAC
PRIMER_RIGHT_1_SEQUENCE=TGTAGGGAAAAAGAAGGGTTGA
PRIMER_LEFT_1=68,22
PRIMER_RIGHT_1=197,22
PRIMER_LEFT_1_TM=59.976
PRIMER_RIGHT_1_TM=59.974
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=0.1191
PRIMER_LEFT_2_PENALTY=0.023556
PRIMER_RIGHT_2_PENALTY=0.095574
PRIMER_LEFT_2_SEQUENCE=CAGACCCAGAAATTCTCCAAAC
PRIMER_RIGHT_2_SEQUENCE=TCGAGAGCTTTCTCTTGAATCC
PRIMER_LEFT_2=68,22
PRIMER_RIGHT_2=367,22
PRIMER_LEFT_2_TM=59.976
PRIMER_RIGHT_2_TM=60.096
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.1229
PRIMER_LEFT_3_PENALTY=0.023556
PRIMER_RIGHT_3_PENALTY=0.099308
PRIMER_LEFT_3_SEQUENCE=CAGACCCAGAAATTCTCCAAAC
PRIMER_RIGHT_3_SEQUENCE=TAAGAGTGGTTTTGGGTGGAGT
PRIMER_LEFT_3=68,22
PRIMER_RIGHT_3=218,22
PRIMER_LEFT_3_TM=59.976
PRIMER_RIGHT_3_TM=59.901
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=0.1229
PRIMER_LEFT_4_PENALTY=0.023556
PRIMER_RIGHT_4_PENALTY=0.099308
PRIMER_LEFT_4_SEQUENCE=ACAGACCCAGAAATTCTCCAAA
PRIMER_RIGHT_4_SEQUENCE=TAAGAGTGGTTTTGGGTGGAGT
PRIMER_LEFT_4=67,22
PRIMER_RIGHT_4=218,22
PRIMER_LEFT_4_TM=59.976
PRIMER_RIGHT_4_TM=59.901
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.8000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=152
=
PRIMER_SEQUENCE_ID=SSR_06187 Ps_contig_mira-and-tgicl-ass_franssen_42269
SEQUENCE=ACGTCGNGGGGATCACAAACACTTACACTAATAATGGAAACGTGGTTCATAGCGCTACTCTCTCTCTGCATCATCTTCCTCATCAGAGCCACCGTCTCCACCCTCACCACCACCACCACCAAAACCGCCACTCTCCCTCCCGGTCCTTTCCACATCCCCGTTCTCACAAGCATCCTATGGCTCCGAAAATCCTTCGCCCAACTCGAACCTTTACTCAAAACCCTCCACGCCAAATATGGTCCTATCATCACTCTCAGAATCGGTTCTCGACCTTCCGTTTTCATCGCCGACCGTTCTCTCGCTCACCACGCTCTTGTTCATAACTCATCAATCTTCTCCGATCGTCCTAAAGCACTTCCCACCGGGAAACTCACGTCCAGCAACCAACATAATATCAGTTCCGCTTCTTACGGTGCCACGTGGAGGACCCTCCGTCGTAATCTTGCCTCCGAGATGCTTAATCCGTCGAAAATCAAATCTTTCTCGGAAATCCGGAACTGGGTCCTGGACGCTCTCATCAACCGTCTCAAAACAGCGTCGGAGTCGGAATCCTTTACGTTTGTTCCTCACTTTCATTACGCCATGTTTTGTTTACTTGTTTTTATGTGTTTTGGTGAAAGACTTAACGATGAGAAAGTTAATGAAATTGAACGTGTACAGAGGAAGCTTCTGTTGAGTATGAGTAGATTCAATGTATTGAATTTCTGGCCTGAGGTTACTAAGATTTTGCTGCGCAAACGATGGGAGGAGTTGTTGAAGTTGCTTAAGGATCAAGAAGATGTTCTTCTTCCGCTGATAAGAGCGAGGAAGCAAAATAAGGAAAGTAAGAGTGTTGTTTCTTATGCGGATACTCTTTTGGAATTGGAGTTAAAGGAGGAGAAACGCAAGTTGACTGAAGAGGAAATGGTTACTCTTTGTTCCGAGTTTTTGAATGGTGGCACGGACACAACTTCGACGGCTTTGCAGTGGATTATGGCGAATTTGGGTAGAAAGTTACGTCCGGGACGTGCAGGAAG
TARGET=58,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5828
PRIMER_LEFT_PENALTY=0.077340
PRIMER_RIGHT_PENALTY=0.505477
PRIMER_LEFT_SEQUENCE=NGGGGATCACAAACACTTACAC
PRIMER_RIGHT_SEQUENCE=TTGAGTAAAGGTTCGAGTTGGG
PRIMER_LEFT=6,22
PRIMER_RIGHT=217,22
PRIMER_LEFT_TM=59.923
PRIMER_RIGHT_TM=60.505
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=10.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=212
PRIMER_PAIR_PENALTY_1=0.6133
PRIMER_LEFT_1_PENALTY=0.077340
PRIMER_RIGHT_1_PENALTY=0.535973
PRIMER_LEFT_1_SEQUENCE=NGGGGATCACAAACACTTACAC
PRIMER_RIGHT_1_SEQUENCE=GATGATAGGACCATATTTGGCG
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=59.923
PRIMER_RIGHT_1_TM=60.536
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=11.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=0.7891
PRIMER_LEFT_2_PENALTY=0.077340
PRIMER_RIGHT_2_PENALTY=0.711791
PRIMER_LEFT_2_SEQUENCE=NGGGGATCACAAACACTTACAC
PRIMER_RIGHT_2_SEQUENCE=GAACCGATTCTGAGAGTGATGA
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=265,22
PRIMER_LEFT_2_TM=59.923
PRIMER_RIGHT_2_TM=59.288
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=260
PRIMER_PAIR_PENALTY_3=1.0800
PRIMER_LEFT_3_PENALTY=0.077340
PRIMER_RIGHT_3_PENALTY=1.002631
PRIMER_LEFT_3_SEQUENCE=NGGGGATCACAAACACTTACAC
PRIMER_RIGHT_3_SEQUENCE=CGAGAACCGATTCTGAGAGTG
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=268,21
PRIMER_LEFT_3_TM=59.923
PRIMER_RIGHT_3_TM=59.997
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=263
PRIMER_PAIR_PENALTY_4=1.0939
PRIMER_LEFT_4_PENALTY=0.077340
PRIMER_RIGHT_4_PENALTY=1.016543
PRIMER_LEFT_4_SEQUENCE=NGGGGATCACAAACACTTACAC
PRIMER_RIGHT_4_SEQUENCE=GCCATAGGATGCTTGTGAGAAC
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=180,22
PRIMER_LEFT_4_TM=59.923
PRIMER_RIGHT_4_TM=61.017
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_06188 Ps_contig_mira-and-tgicl-ass_franssen_42269
SEQUENCE=ACGTCGNGGGGATCACAAACACTTACACTAATAATGGAAACGTGGTTCATAGCGCTACTCTCTCTCTGCATCATCTTCCTCATCAGAGCCACCGTCTCCACCCTCACCACCACCACCACCAAAACCGCCACTCTCCCTCCCGGTCCTTTCCACATCCCCGTTCTCACAAGCATCCTATGGCTCCGAAAATCCTTCGCCCAACTCGAACCTTTACTCAAAACCCTCCACGCCAAATATGGTCCTATCATCACTCTCAGAATCGGTTCTCGACCTTCCGTTTTCATCGCCGACCGTTCTCTCGCTCACCACGCTCTTGTTCATAACTCATCAATCTTCTCCGATCGTCCTAAAGCACTTCCCACCGGGAAACTCACGTCCAGCAACCAACATAATATCAGTTCCGCTTCTTACGGTGCCACGTGGAGGACCCTCCGTCGTAATCTTGCCTCCGAGATGCTTAATCCGTCGAAAATCAAATCTTTCTCGGAAATCCGGAACTGGGTCCTGGACGCTCTCATCAACCGTCTCAAAACAGCGTCGGAGTCGGAATCCTTTACGTTTGTTCCTCACTTTCATTACGCCATGTTTTGTTTACTTGTTTTTATGTGTTTTGGTGAAAGACTTAACGATGAGAAAGTTAATGAAATTGAACGTGTACAGAGGAAGCTTCTGTTGAGTATGAGTAGATTCAATGTATTGAATTTCTGGCCTGAGGTTACTAAGATTTTGCTGCGCAAACGATGGGAGGAGTTGTTGAAGTTGCTTAAGGATCAAGAAGATGTTCTTCTTCCGCTGATAAGAGCGAGGAAGCAAAATAAGGAAAGTAAGAGTGTTGTTTCTTATGCGGATACTCTTTTGGAATTGGAGTTAAAGGAGGAGAAACGCAAGTTGACTGAAGAGGAAATGGTTACTCTTTGTTCCGAGTTTTTGAATGGTGGCACGGACACAACTTCGACGGCTTTGCAGTGGATTATGGCGAATTTGGGTAGAAAGTTACGTCCGGGACGTGCAGGAAG
TARGET=105,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4008
PRIMER_LEFT_PENALTY=0.095924
PRIMER_RIGHT_PENALTY=0.304841
PRIMER_LEFT_SEQUENCE=TCTCTCTGCATCATCTTCCTCA
PRIMER_RIGHT_SEQUENCE=TGAGTTATGAACAAGAGCGTGG
PRIMER_LEFT=60,22
PRIMER_RIGHT=326,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=60.305
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=267
PRIMER_PAIR_PENALTY_1=0.5185
PRIMER_LEFT_1_PENALTY=0.342857
PRIMER_RIGHT_1_PENALTY=0.175676
PRIMER_LEFT_1_SEQUENCE=ACGTGGTTCATAGCGCTACTCT
PRIMER_RIGHT_1_SEQUENCE=GTTATGAACAAGAGCGTGGTGA
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=323,22
PRIMER_LEFT_1_TM=60.343
PRIMER_RIGHT_1_TM=60.176
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.5374
PRIMER_LEFT_2_PENALTY=0.361767
PRIMER_RIGHT_2_PENALTY=0.175676
PRIMER_LEFT_2_SEQUENCE=TGCATCATCTTCCTCATCAGAG
PRIMER_RIGHT_2_SEQUENCE=GTTATGAACAAGAGCGTGGTGA
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=323,22
PRIMER_LEFT_2_TM=60.362
PRIMER_RIGHT_2_TM=60.176
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=258
PRIMER_PAIR_PENALTY_3=0.5374
PRIMER_LEFT_3_PENALTY=0.361767
PRIMER_RIGHT_3_PENALTY=0.175676
PRIMER_LEFT_3_SEQUENCE=CTGCATCATCTTCCTCATCAGA
PRIMER_RIGHT_3_SEQUENCE=GTTATGAACAAGAGCGTGGTGA
PRIMER_LEFT_3=65,22
PRIMER_RIGHT_3=323,22
PRIMER_LEFT_3_TM=60.362
PRIMER_RIGHT_3_TM=60.176
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.5828
PRIMER_LEFT_4_PENALTY=0.077340
PRIMER_RIGHT_4_PENALTY=0.505477
PRIMER_LEFT_4_SEQUENCE=NGGGGATCACAAACACTTACAC
PRIMER_RIGHT_4_SEQUENCE=TTGAGTAAAGGTTCGAGTTGGG
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=59.923
PRIMER_RIGHT_4_TM=60.505
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=10.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_06189 Ps_contig_mira-and-tgicl-ass_franssen_42271
SEQUENCE=CGCGGGGATATCAGATTATCAGGTACCCACTATTGCTTCNACCCAAAAACGCTAACCGCATAAGACTCGGATTTTCTCCCTAAGCAATTTTCCACTTCTTCTTGCTCAAGAAAAAAGCGAAACGCAGAAGCTTAAAAAAAGTTGAAGCAACAAAGTAGCAAATTTCCTCCCCCTACATATGAAGTTCATTTCCGAAGGAAGCAATCACTTTCATGAAGATTGCTCAACTTTGATTTTGCCTGCTGTGTCTATTGGGAATGTTGGGCAGTTAACAGCGGATCTTTTGGTTTCGTCATTGGGTTCTGAGAAAGTTGGTTACTTAGACGATCCTTACGTTCTTCCCTGTGTTGGCAATGATGCTTATGGACCTGTTCCTCAAGGGGACCTTGCACTTCCTCTTGAAGCTTATGATTCCCCTTCTAATGGTGTCACTGTTATCCAGCAGAGATCTCCTGTAATTAAGGGAATGATGCTCGAGTTTGCCAAAAACATGGCTGATTTTATTGCCGGAAGTGGAAAAAAGCATATTATTATTCTCTCCAGCTTAGATTTTGGGAAGTGGCAAAAGATTGACATGTCAAGTGGTTTGCAGATTTATTACCTATCCAGTGCCAACAGCAACGGAACGGACGAAAACTGTGAACAGCTTGGATGGAAGAAACTTCAGGAGTATGACCCTTCTCAAAAGCATTGGAAATATCTTAGTGATTTAGCTGAAGGAAAAGCAACTACGGAAGATACTATTTCTATAGAAGATGAACTAGAAGAAGAAGATTATTATGCTAGCTTGCCTTTCGCTGCACTTTTTTCTTTTCTCAAGGCTAAAGGTTTGAAGGTCACCTGCTTGTTATGTTATTGCTCAGAAGGAGACAACACATCTGATGCTTTTCAATTAGCTGATGCAGCATGCAAACTTTTAAAGCCAAGTCAGCCTAATTCGGGAATTGAAGGTGGCAAATGGCGAGTTCCACTTTCTTGGATGTCTGTGTATGGACCACCTCCAGATGTGTCCATCTTCTAAGATTAGGTTAATGTAATTAGTAAAGTTAAAACGGCGACTGAAAATGGATATTATTGTCACTTTTTTTACACAACATATCTGTTCCGTGTCGTTACTATTTTCACAGTACAGGTGCTAGTAATAGAACTTCAGTTTTTGTTTTTTTGTCTT
TARGET=763,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1613
PRIMER_LEFT_PENALTY=0.075346
PRIMER_RIGHT_PENALTY=0.085951
PRIMER_LEFT_SEQUENCE=AGCTGAAGGAAAAGCAACTACG
PRIMER_RIGHT_SEQUENCE=ATGTGTTGTCTCCTTCTGAGCA
PRIMER_LEFT=711,22
PRIMER_RIGHT=877,22
PRIMER_LEFT_TM=60.075
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.1884
PRIMER_LEFT_1_PENALTY=0.102490
PRIMER_RIGHT_1_PENALTY=0.085951
PRIMER_LEFT_1_SEQUENCE=CTTGGATGGAAGAAACTTCAGG
PRIMER_RIGHT_1_SEQUENCE=ATGTGTTGTCTCCTTCTGAGCA
PRIMER_LEFT_1=646,22
PRIMER_RIGHT_1=877,22
PRIMER_LEFT_1_TM=60.102
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=232
PRIMER_PAIR_PENALTY_2=0.2239
PRIMER_LEFT_2_PENALTY=0.075346
PRIMER_RIGHT_2_PENALTY=0.148509
PRIMER_LEFT_2_SEQUENCE=AGCTGAAGGAAAAGCAACTACG
PRIMER_RIGHT_2_SEQUENCE=CACAGACATCCAAGAAAGTGGA
PRIMER_LEFT_2=711,22
PRIMER_RIGHT_2=987,22
PRIMER_LEFT_2_TM=60.075
PRIMER_RIGHT_2_TM=60.149
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.4000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=277
PRIMER_PAIR_PENALTY_3=0.2906
PRIMER_LEFT_3_PENALTY=0.075346
PRIMER_RIGHT_3_PENALTY=0.215215
PRIMER_LEFT_3_SEQUENCE=AGCTGAAGGAAAAGCAACTACG
PRIMER_RIGHT_3_SEQUENCE=TAACAAGCAGGTGACCTTCAAA
PRIMER_LEFT_3=711,22
PRIMER_RIGHT_3=849,22
PRIMER_LEFT_3_TM=60.075
PRIMER_RIGHT_3_TM=59.785
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=7.3000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=0.3177
PRIMER_LEFT_4_PENALTY=0.102490
PRIMER_RIGHT_4_PENALTY=0.215215
PRIMER_LEFT_4_SEQUENCE=CTTGGATGGAAGAAACTTCAGG
PRIMER_RIGHT_4_SEQUENCE=TAACAAGCAGGTGACCTTCAAA
PRIMER_LEFT_4=646,22
PRIMER_RIGHT_4=849,22
PRIMER_LEFT_4_TM=60.102
PRIMER_RIGHT_4_TM=59.785
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=204
=
PRIMER_SEQUENCE_ID=SSR_06190 Ps_contig_mira-and-tgicl-ass_franssen_42277
SEQUENCE=CTGGTGTGCTCATCCTGATATGGGACAATCTAGCCCTTCTCCCCCAGACTTATTTATTTATGGTGGTGGAAGCTATCTGGATGCTAACACCAAATAGGTGATACCGACTTACTACAACCTACCAAAAAAATTATTTATCTTTAGAATCACAAGATACATGTAAATGTCAACCACAAACAATTGGAAGTGCAGAAGCAGCTTCTTGACATCATCCGAGRCAAAGAGTTACCTGGCTAAAGTCTCTTATAGATCAAGAGTTATACTTCTGTTTCAAAAGATTGAGAGAGCATATATATGCATTTTTGGACTTTATGCCTAGGAGTTTGGCTCTGAATGCGGCAATCCGGACTAGTGTTGTGTGTACATTTCATATCTTGATTTTGTAAAATATTTTAGGTCTGAAAG
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06191 Ps_contig_mira-and-tgicl-ass_franssen_42279
SEQUENCE=ATGCTAAGCCTCTTAGTATTTTCTTCCCAAGATTAAGAACGGTGAAATCCATACCATTCAGTGGAACTGGCAAGCACAAATATAGTTTCCAGAATATTAAAGTTCCTCCTCTCCCTGTGCATTTTTGTGATTGTGAGGAACATTGTAGTCCTTTTATCCCTAAAAGGAAAAGTTATCTATGAATACGGCAGTTGAAACTGGTGGAATTAATGATGAAGAAATTAACATCATGAATGCTGTGATGAGCAAGCTTTTTGAAAAAGAGAAGGTTTCTAACACCAAGCAACTTGGAAAGAAGCATGATTCATTTGAATCTCCTGATGCTTTACAATCTAATAAAGGTGAAGTAGAAAGTGCAACAGATGTAGATGTTGATATTGATGATGATGATGATGATGATGATGATGATGATGA
TARGET=379,36
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=TARGET beyond end of sequence
=
PRIMER_SEQUENCE_ID=SSR_06192 Ps_contig_mira-and-tgicl-ass_franssen_42288
SEQUENCE=CAATTCAAATTAAAAACAGCTTATCAAATAAGTCTACTTTAAAAATTCTTGGAAAATAATTAATAGAAAGAGCAGATTAATGTGTTACACAATAAATCAAAGGAAGCACTCAGAAACACACTGCATTATCCAAATAAACAACTTTAAATCCATAGATTAATCATCCCACAAAATCTGGAAATAGGATCCAACAAGTTATCTCACAGAAACCAACATTCATGTATATAAACCACAAAACTGATTAACAGTAACAAGTTTTGATAAAAATCCAAAAGAAATTTTTNAAGTTTTTTCTCTGTTAACTCCAAAGAGACACAACACAGTAAGCTATTTCTTCTAAAAAAAACCTTAAACCACAACAACAACAAAGAATCACTTTTTTTCCTAAAACCAGAACGGCATGGCAAGAACAAACGCANCAAAGTCTTTTTTTTTCAAAACTCTAAAACAACAACAGCAACCACACACAAAGCTATTTCTTACTAAAAACCCTAGAACACACAACATAAACATAGCAAAAAAAGCAATTCTTCTCAAAGAGCACCATAATCACACCTTACCAGAAGCAGATCGATAGTGAAAGAAAAGTAAGAGGAAGG
TARGET=356,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0366
PRIMER_LEFT_PENALTY=0.176730
PRIMER_RIGHT_PENALTY=0.859847
PRIMER_LEFT_SEQUENCE=CCCACAAAATCTGGAAATAGGA
PRIMER_RIGHT_SEQUENCE=GACTTTGNTGCGTTTGTTCTTG
PRIMER_LEFT=164,22
PRIMER_RIGHT=425,22
PRIMER_LEFT_TM=60.177
PRIMER_RIGHT_TM=60.860
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=262
PRIMER_PAIR_PENALTY_1=1.0366
PRIMER_LEFT_1_PENALTY=0.176730
PRIMER_RIGHT_1_PENALTY=0.859847
PRIMER_LEFT_1_SEQUENCE=TCCCACAAAATCTGGAAATAGG
PRIMER_RIGHT_1_SEQUENCE=GACTTTGNTGCGTTTGTTCTTG
PRIMER_LEFT_1=163,22
PRIMER_RIGHT_1=425,22
PRIMER_LEFT_1_TM=60.177
PRIMER_RIGHT_1_TM=60.860
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=1.8324
PRIMER_LEFT_2_PENALTY=1.812450
PRIMER_RIGHT_2_PENALTY=0.019966
PRIMER_LEFT_2_SEQUENCE=TCTGGAAATAGGATCCAACAAG
PRIMER_RIGHT_2_SEQUENCE=GTGTGGTTGCTGTTGTTGTTTT
PRIMER_LEFT_2=173,22
PRIMER_RIGHT_2=465,22
PRIMER_LEFT_2_TM=58.188
PRIMER_RIGHT_2_TM=59.980
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=1.8520
PRIMER_LEFT_3_PENALTY=0.016921
PRIMER_RIGHT_3_PENALTY=1.835060
PRIMER_LEFT_3_SEQUENCE=AGCACTCAGAAACACACTGCAT
PRIMER_RIGHT_3_SEQUENCE=ATGCCGTTCTGGTTTTAGGAA
PRIMER_LEFT_3=104,22
PRIMER_RIGHT_3=401,21
PRIMER_LEFT_3_TM=59.983
PRIMER_RIGHT_3_TM=60.835
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.4000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=298
PRIMER_PAIR_PENALTY_4=1.9384
PRIMER_LEFT_4_PENALTY=0.103353
PRIMER_RIGHT_4_PENALTY=1.835060
PRIMER_LEFT_4_SEQUENCE=GAAGCACTCAGAAACACACTGC
PRIMER_RIGHT_4_SEQUENCE=ATGCCGTTCTGGTTTTAGGAA
PRIMER_LEFT_4=102,22
PRIMER_RIGHT_4=401,21
PRIMER_LEFT_4_TM=60.103
PRIMER_RIGHT_4_TM=60.835
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=300
=
PRIMER_SEQUENCE_ID=SSR_06193 Ps_contig_mira-and-tgicl-ass_franssen_42297
SEQUENCE=TAACAAAAAYTCGAACCTCACACCATTTCATCATGTGATATCCTTCTGTCCTCTCAAATGCCTCGCTGGAAAACTCAATAGCTCTGCCAAGTCGGAGCTCATCGCCGGCGGCGGCGGAGCTCGCCATACCACCACCTTTACATTCTTTTAACCATCGCAACGCCCTTGTTTCAAATCCAACAACAATATCTTCAAATATTCACTCTACAGAAAAATCGAAGCTAAGTAAGTCGAGCCAAGAGAGGTGATATTATCGCGGTGACGGAAGAAAACTCAGGCAATGCTACATATGGTACGCACATGATGGCCACTTCTTTGAAAGATGATTGATGAATACAATGATGCAACCCTTTAAAGATGATGAAGTCCTTTGAAGAAGCTCACTTTTTAATAGGATCCCATTCTTGTAGTGAGTCTTTATTGTAATGGATAGTGTTTTGATTACAAGATTTTACCAGCTTTTTTAACAATTCTAATCAATGTGTCAAACCTTTTTATTTGTCTTGTAACTTTGACTTCATGTAATAATGAACATTCTTGACCTTTTGGAGGAACATTTCTTATATGTGGCATGCTTGAATCAACAACAAACTAAATACATTAGAATTGGAATGAACTTCAGTGG
TARGET=106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06194 Ps_contig_mira-and-tgicl-ass_franssen_42307
SEQUENCE=GTGGTATCAACGCAGAGTAATGGAGTCTGTCACTCAAGCAAGCAAGGTGGTGTGCGAGGTAGTATTATGGAATTTGGAGTATTGTTGGACCATTTGTTAAGTTTTTTTTTTCCCTTTTTCTTTCCAATTTCTGGTTAAGAGCTGTGAAACATGAGAGGTTGCATATGGCCTTGCATTTGAGGATGCACTATGGAAATTGGGATATTGTTGGAACCTTTGTTTAGTGTTTTATTTTTAATGAAAAGTTTCTGGATGAGAGCCGGGAACCTGAAGACATTAGAACCTCCAGAGAATTTGGTTTTGGCTTTTAACTGCCATATGTTCTGGTGGGTCCAGTTGGTGTTTCTGTTGGGTTACATTTGCAGGTGTTACTGCATGTTATCAGCACTGTGACTGCATGTTACCAGTACTGTTACTTTCAAATTAAATAAATGGTTTAGAGATTAATTTACCTTGTYAATGTAATTCCG
TARGET=35,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06195 Ps_contig_mira-and-tgicl-ass_franssen_42308
SEQUENCE=AGTACCATCTGGATGGTTTATCCAATCGGCCATCGTTGATAGTCGCAAATAAGATTGATGAGGAAGGTGCAGAGGAAGTGTATGAAGAGTTAAAGAGAAGGATCGAGGGTGTTCCTATCTTTCCTGTTAGTGCTGTTTTGGAGGAAGGGATACCAGAGCTTAAAGCCGGCCTTAGAATGCTTGTCAATGGTGAAACTTCATGCACACTTTGCCTAGATCAAATTTTGTCTTGATTAGATTAGATTATCATTAA
TARGET=232,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06196 Ps_contig_mira-and-tgicl-ass_franssen_42316
SEQUENCE=AGTTTTTTTAATTCCAAATCCATGTCTTTCATATATTCAGAATCAGCTGCTGACATTTAGCAGTAAAGGAAAAATGTTAAACTGCAGCAAATTATTTTATTCCTCAATTACAAATGCTATTAGTTTTAATTATACAAAAAACATGTCTTCACATGATTGATTATGTTCCTATCATCCAAAGACAGTTATAGTATTGCTGCCAATGTGTCTCTGCGTCTGGTGTCGTCTCTATGTAAACTACATTTCTGACCAATCAGCAGCTTGAAAAATAATAAATTGAAAGATAGATAGATAGAGAGACACAGAGATCAGGTTTTGAACTTATGAGTGAACTTTTGGGTGATTTTCTCAACTCCCTCCCTACAATCGCCAGCAACTCGCTTTCCACCTTCTTTCACAGTAAATCCAAACTCGATGGCACTATGCTGCAACTCATTGGCTGACTTACCTGCCTTTGAGACAGCAGTGTCACGCAATTCTTCAGCCTGCAATTTTGTTCTGGATGCCCATTCCTTCAAGTTTGATATAAATATGGAAATCATGAGAATGATCTTGTCAATTGTTTCTCGGGATTTTCCTCTGACATCTGCACCCATTCGTTTCAGCTTCTCCAATAAG
TARGET=282,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7803
PRIMER_LEFT_PENALTY=0.729838
PRIMER_RIGHT_PENALTY=0.050443
PRIMER_LEFT_SEQUENCE=GTATTGCTGCCAATGTGTCTCT
PRIMER_RIGHT_SEQUENCE=GCTGTCTCAAAGGCAGGTAAGT
PRIMER_LEFT=190,22
PRIMER_RIGHT=463,22
PRIMER_LEFT_TM=59.270
PRIMER_RIGHT_TM=59.950
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.7803
PRIMER_LEFT_1_PENALTY=0.729838
PRIMER_RIGHT_1_PENALTY=0.050443
PRIMER_LEFT_1_SEQUENCE=AGTATTGCTGCCAATGTGTCTC
PRIMER_RIGHT_1_SEQUENCE=GCTGTCTCAAAGGCAGGTAAGT
PRIMER_LEFT_1=189,22
PRIMER_RIGHT_1=463,22
PRIMER_LEFT_1_TM=59.270
PRIMER_RIGHT_1_TM=59.950
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=275
PRIMER_PAIR_PENALTY_2=0.8162
PRIMER_LEFT_2_PENALTY=0.729838
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=GTATTGCTGCCAATGTGTCTCT
PRIMER_RIGHT_2_SEQUENCE=TGTCTCAAAGGCAGGTAAGTCA
PRIMER_LEFT_2=190,22
PRIMER_RIGHT_2=461,22
PRIMER_LEFT_2_TM=59.270
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.8162
PRIMER_LEFT_3_PENALTY=0.729838
PRIMER_RIGHT_3_PENALTY=0.086339
PRIMER_LEFT_3_SEQUENCE=AGTATTGCTGCCAATGTGTCTC
PRIMER_RIGHT_3_SEQUENCE=TGTCTCAAAGGCAGGTAAGTCA
PRIMER_LEFT_3=189,22
PRIMER_RIGHT_3=461,22
PRIMER_LEFT_3_TM=59.270
PRIMER_RIGHT_3_TM=59.914
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.8323
PRIMER_LEFT_4_PENALTY=0.729838
PRIMER_RIGHT_4_PENALTY=0.102490
PRIMER_LEFT_4_SEQUENCE=GTATTGCTGCCAATGTGTCTCT
PRIMER_RIGHT_4_SEQUENCE=TGTAGGGAGGGAGTTGAGAAAA
PRIMER_LEFT_4=190,22
PRIMER_RIGHT_4=364,22
PRIMER_LEFT_4_TM=59.270
PRIMER_RIGHT_4_TM=60.102
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=175
=
PRIMER_SEQUENCE_ID=SSR_06197 Ps_contig_mira-and-tgicl-ass_franssen_42319
SEQUENCE=CTCACACTTCATCTCTCTCTCTCTCTCTAAAATGTCTCCTATAGCTACTCTCGTTACCACTCTCTTCCTCATCATATCTCTCCAAACACAAGCTTCCGTTCACGATTACAGAAGCGAGACTTTCTCTGTCAAAGGCAACGCCTTTGTCGTCCATGGAGGCAGCGAAGGAATTTACTCATCTACCCTCAATGAAACTTCCGTCTCACCTTCAATTCCTGACTCTTTCATTAGGTTTGATAAAGTCACATTCCGTAGAAACAAGGAATTATCAAACTTTAGCTCATGGCCGATTCAAGCTGTTGTTTTTGAAGTTGAAGATAGAGAGATGATTGGTGGTTCGGCCTATGGGGGTCAACGGGCCGTTTGTTGCACCGGTGATCTGGCTAAACTCGGTGTGTGCAATGAAGGACGTGTCATTCACCGTCCTTCTACCGTGAATTTGGATTGGCCTCAAGTTTTTGGTGTTGCTTTCGAGATGGACGATGAAGTGGCAGAGCTGCCGTTGAAATCTATACAGATCACGAAAACCGGGATGTACAATTTGTATTTCATTCATTGTGATCCGAGGCTTAAGGAATTGATTGTAGAAGGCAAAACTGTATGGAAGAATCCCTCGGGTTATCTTCCTGGTA
TARGET=12,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06198 Ps_contig_mira-and-tgicl-ass_franssen_42323
SEQUENCE=TTCGGGGGAAAATAAGAAAATTGTCAATACCCGATAAAGACAAATTCGCCATCTTCCTTCGTTGTCGATTCTTCTTCTTCATCTTCTATATCACGCGAAGTCATCGAAGCCCATTCAAACTCACATCACAAATTCCACTCTTTTTGATGAAAGAGTTTCTTCTTCTTGTTGTGGTCTCTTTGGCCTTTCGATTGACCCTCTCAGTCTCAGTGTCAGAGTCTCAATCTGTTTCACTATTGCCTCATGCTCATGAATCCTTCAATTTCAGTTATATTCGGATGAAGAACGAGGCAAGTTGTTCTTACTTGGTGGTTATATCTACTAGCTGTTCGTCTCCTAGGTATACGAGGGATCAAATCAGTATTTCTTTTGGTGATGCTTATGGGAATCAGATAAATGCACCAAGACTAGATGATCCATCTTCTGGTACATTTGAGAGCTGTTCTTCCGATACGTTTCAGATAAATGGTCCATGTGCGTATCAGATATGTTATGTGTACCTCTATAGATCTGGCTCAGATGGTTGGAAACCCGACACTGTGAAAATCAATGGTAATAGTGGCAGTCCTGTTACTTTCTATTACAATACTTTCATTCCCAGAGATACATGGTACGGGTTTAATTCATGCAACGATGCTGCTTCTTCATACAAAGTAACTGCGCAGAAATTGCTAATGTTAGTTATTCTAGGAATTGTTTTGAGTTTCTGGTTATAAGTTTATGTCCTTTTCATCCCTTACATATCCTATAGATGTAGCCATGAAATATGCAGTTAAGTATTCTCTCCGGTCTTAAGTATAAGTAAAAGTCAAAATGTACTTCGTCCCTTACATTTCTTTATACTTGTAGCCATGAAACTTTGTAGTCAAGTATTGTATGTGTAGAAGTAGTTGAGACAGGAGATGTGGAAGACCCTTGGCCAAGTTGGTTAATTAGTTAATTATTTGGCTTTGTAGTTTCTTTTCAAATTCTTTGTTTTATTGATGAAATTATGTAGTTTAAGGTTGGAGTTGCGAATTGGACACCTTGATATGCTGTGTTTGAAGTTTTTCTTA
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3912
PRIMER_LEFT_PENALTY=0.324383
PRIMER_RIGHT_PENALTY=0.066849
PRIMER_LEFT_SEQUENCE=TACCCGATAAAGACAAATTCGC
PRIMER_RIGHT_SEQUENCE=GATTCATGAGCATGAGGCAATA
PRIMER_LEFT=27,22
PRIMER_RIGHT=255,22
PRIMER_LEFT_TM=60.324
PRIMER_RIGHT_TM=60.067
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=229
PRIMER_PAIR_PENALTY_1=0.4961
PRIMER_LEFT_1_PENALTY=0.324383
PRIMER_RIGHT_1_PENALTY=0.171757
PRIMER_LEFT_1_SEQUENCE=TACCCGATAAAGACAAATTCGC
PRIMER_RIGHT_1_SEQUENCE=AACAACTTGCCTCGTTCTTCAT
PRIMER_LEFT_1=27,22
PRIMER_RIGHT_1=299,22
PRIMER_LEFT_1_TM=60.324
PRIMER_RIGHT_1_TM=60.172
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.5498
PRIMER_LEFT_2_PENALTY=0.324383
PRIMER_RIGHT_2_PENALTY=0.225403
PRIMER_LEFT_2_SEQUENCE=TACCCGATAAAGACAAATTCGC
PRIMER_RIGHT_2_SEQUENCE=GCATGAGGCAATAGTGAAACAG
PRIMER_LEFT_2=27,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=60.324
PRIMER_RIGHT_2_TM=59.775
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=10.2000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=0.5498
PRIMER_LEFT_3_PENALTY=0.324383
PRIMER_RIGHT_3_PENALTY=0.225403
PRIMER_LEFT_3_SEQUENCE=TACCCGATAAAGACAAATTCGC
PRIMER_RIGHT_3_SEQUENCE=AGCATGAGGCAATAGTGAAACA
PRIMER_LEFT_3=27,22
PRIMER_RIGHT_3=247,22
PRIMER_LEFT_3_TM=60.324
PRIMER_RIGHT_3_TM=59.775
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.2000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=221
PRIMER_PAIR_PENALTY_4=0.5711
PRIMER_LEFT_4_PENALTY=0.324383
PRIMER_RIGHT_4_PENALTY=0.246726
PRIMER_LEFT_4_SEQUENCE=TACCCGATAAAGACAAATTCGC
PRIMER_RIGHT_4_SEQUENCE=AGACTGAGAGGGTCAATCGAAA
PRIMER_LEFT_4=27,22
PRIMER_RIGHT_4=206,22
PRIMER_LEFT_4_TM=60.324
PRIMER_RIGHT_4_TM=60.247
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=10.2000
PRIMER_RIGHT_4_END_STABILITY=9.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=180
=
PRIMER_SEQUENCE_ID=SSR_06199 Ps_contig_mira-and-tgicl-ass_franssen_42328
SEQUENCE=AAGGTTTAAAAAGTTTAGTTNYTTTTTCTGAATTATTTTGTAATCAAGAAACAAAAATGATAAATCGCACTACCACATATACACATTCTCAAATGACAAATCTAAATAATCTCTCTCAAATCATTACGGAAAGACTATTATTAATCCAACAGAAACAAAGACGACATAACCTAAGTAACCAACTACACCTAATATAAAAATAAAAATAAAGTTATTTCTACTTTAGCTGGACCTATACCATGATGATTCCTCTGTGATGCAACTACCAACAAACGTTAAACCACCGTCCCATCTTTTAACCACGTTTCCATTAAACCCAATCAAATCTTGACACGTGTACCACCCATTCTCATTCTCTCCGTTTCTCAACCTCAACACCTTCTCCCATCGTTTCCACCACTCCGTCTCATCCTCCGGTCCCCACCCTCCCGCAACCGGCATCAGCCCGTAGCCACAACCACCACCACTCTCTCCACCACCGCATTGCATATCTTCCTCGTAATACACCGTATACTTCCCCTTCCTCACACCATCAACATCATCATCATCATTCCAAATCTCGTTTAATGTTTTCCTCGGTTTCTCAGCTTCCGACATCAACGCTTCAACTTCCGGGTCGGTTTTAACCGAAACCGGCTCTTGTAATTTAGAACAACCCGATGAGCGAATCTTCCAGAAGCTGAGAGCGGCGGTTAGGAGAGCTAGACAGGTCCATAAATTGTTGAGAACTGTGACTAAACCGAAGTTCAAGTAGTTGAAAGCTAAAGCCTCAACGTTGGAATCTAACAAGACGTTCATTGTTTTTTGTTTTTGTTTTGGTTTGAGGTTTGTGTTGGAGAGAAAAATGAATGAATTGAAGAATAAGGGAAAGGGATTGAGGATGGTGAAGATATAAGGAGTGGATGTGTGTGTGGGATCTTGATAAGATGCCTAGAATCGATGTCGAAGATGCGTATTTTCTAGGTNTCCCCC
TARGET=537,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06200 Ps_contig_mira-and-tgicl-ass_franssen_42328
SEQUENCE=AAGGTTTAAAAAGTTTAGTTNYTTTTTCTGAATTATTTTGTAATCAAGAAACAAAAATGATAAATCGCACTACCACATATACACATTCTCAAATGACAAATCTAAATAATCTCTCTCAAATCATTACGGAAAGACTATTATTAATCCAACAGAAACAAAGACGACATAACCTAAGTAACCAACTACACCTAATATAAAAATAAAAATAAAGTTATTTCTACTTTAGCTGGACCTATACCATGATGATTCCTCTGTGATGCAACTACCAACAAACGTTAAACCACCGTCCCATCTTTTAACCACGTTTCCATTAAACCCAATCAAATCTTGACACGTGTACCACCCATTCTCATTCTCTCCGTTTCTCAACCTCAACACCTTCTCCCATCGTTTCCACCACTCCGTCTCATCCTCCGGTCCCCACCCTCCCGCAACCGGCATCAGCCCGTAGCCACAACCACCACCACTCTCTCCACCACCGCATTGCATATCTTCCTCGTAATACACCGTATACTTCCCCTTCCTCACACCATCAACATCATCATCATCATTCCAAATCTCGTTTAATGTTTTCCTCGGTTTCTCAGCTTCCGACATCAACGCTTCAACTTCCGGGTCGGTTTTAACCGAAACCGGCTCTTGTAATTTAGAACAACCCGATGAGCGAATCTTCCAGAAGCTGAGAGCGGCGGTTAGGAGAGCTAGACAGGTCCATAAATTGTTGAGAACTGTGACTAAACCGAAGTTCAAGTAGTTGAAAGCTAAAGCCTCAACGTTGGAATCTAACAAGACGTTCATTGTTTTTTGTTTTTGTTTTGGTTTGAGGTTTGTGTTGGAGAGAAAAATGAATGAATTGAAGAATAAGGGAAAGGGATTGAGGATGGTGAAGATATAAGGAGTGGATGTGTGTGTGGGATCTTGATAAGATGCCTAGAATCGATGTCGAAGATGCGTATTTTCTAGGTNTCCCCC
TARGET=904,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06201 Ps_contig_mira-and-tgicl-ass_franssen_42341
SEQUENCE=ATTRAAGAAAAAAAACAGATAAATATATTATAGAACCAAAACTGAACTTAGATTCATTCATTTCTTACAACACACTCATAATTCTCTCATTCTAGAGCAACAATAATCAGTAGCTGAAAATAAAAATTTACATTTATGAAAAGGTTAGGCTCATACTGATAGATTGGATTTGGATTGGGATCCTAAACCTAAAACAATAATCTACTAAATTCTAAAATTAAAAAAGATAGGGCAAAGATTTTTAGGGTTTAATTTAGGCATAAACGGATCCATAAGATAACGACATCAAAAGCACCGCAGCTTTTTCTTCTTCTCCCAATTTCTTCCAATGCGATCTCTGCATAAACACTTCTTTCCCCAAATTCAATAGTCTCTGCTTCATGCTCAAATTATCCCCAACCTTGCTTCCACCGCTTCCATTGCTCTTTTTCCCCTTTCTCGCTCCGTCTTCGTTGTTTCCTTTGCTTATCCCCAAAATCGCTCTCTTCTTCTTCCTGCTCCTGATCCCGCAAGCATTACACAGAGACTTTGGTCCTGCAGGTCCGCCTCTCCATAGAGGAGTTTTGGAAGTTCCGCAATCGGCGCAGGTTTTCTTCGGACTGTTTTCCGATGAAACGGCGTTAGGGTTTGAATCTTCACCTTCTGATCCCTTTCCAGTATGATCAACCACCATTCTGATTCGATTCTGATCAAACACAAACGCGATTCCGATGACTGAAACAGAATTGGATGAATCAGAATGGAAGATCGAGCATGCAAGATTGAGAAAATAATGGTGATGATGATGATGATGAAGTGACTGTTTAGTAGAGAGAGAGAGGAGGGGAGAGATTTATAGAGAGAGAGAGATTGGGAGAGATAGGAATCGTAAAGAGACACTGATATACTCCTAGAGACACACGCACGCACCAGCCGCCCCGCGTACTCTGTCGTTGACTACCACTGTCTT
TARGET=777,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06202 Ps_contig_mira-and-tgicl-ass_franssen_42341
SEQUENCE=ATTRAAGAAAAAAAACAGATAAATATATTATAGAACCAAAACTGAACTTAGATTCATTCATTTCTTACAACACACTCATAATTCTCTCATTCTAGAGCAACAATAATCAGTAGCTGAAAATAAAAATTTACATTTATGAAAAGGTTAGGCTCATACTGATAGATTGGATTTGGATTGGGATCCTAAACCTAAAACAATAATCTACTAAATTCTAAAATTAAAAAAGATAGGGCAAAGATTTTTAGGGTTTAATTTAGGCATAAACGGATCCATAAGATAACGACATCAAAAGCACCGCAGCTTTTTCTTCTTCTCCCAATTTCTTCCAATGCGATCTCTGCATAAACACTTCTTTCCCCAAATTCAATAGTCTCTGCTTCATGCTCAAATTATCCCCAACCTTGCTTCCACCGCTTCCATTGCTCTTTTTCCCCTTTCTCGCTCCGTCTTCGTTGTTTCCTTTGCTTATCCCCAAAATCGCTCTCTTCTTCTTCCTGCTCCTGATCCCGCAAGCATTACACAGAGACTTTGGTCCTGCAGGTCCGCCTCTCCATAGAGGAGTTTTGGAAGTTCCGCAATCGGCGCAGGTTTTCTTCGGACTGTTTTCCGATGAAACGGCGTTAGGGTTTGAATCTTCACCTTCTGATCCCTTTCCAGTATGATCAACCACCATTCTGATTCGATTCTGATCAAACACAAACGCGATTCCGATGACTGAAACAGAATTGGATGAATCAGAATGGAAGATCGAGCATGCAAGATTGAGAAAATAATGGTGATGATGATGATGATGAAGTGACTGTTTAGTAGAGAGAGAGAGGAGGGGAGAGATTTATAGAGAGAGAGAGATTGGGAGAGATAGGAATCGTAAAGAGACACTGATATACTCCTAGAGACACACGCACGCACCAGCCGCCCCGCGTACTCTGTCGTTGACTACCACTGTCTT
TARGET=809,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06203 Ps_contig_mira-and-tgicl-ass_franssen_42341
SEQUENCE=ATTRAAGAAAAAAAACAGATAAATATATTATAGAACCAAAACTGAACTTAGATTCATTCATTTCTTACAACACACTCATAATTCTCTCATTCTAGAGCAACAATAATCAGTAGCTGAAAATAAAAATTTACATTTATGAAAAGGTTAGGCTCATACTGATAGATTGGATTTGGATTGGGATCCTAAACCTAAAACAATAATCTACTAAATTCTAAAATTAAAAAAGATAGGGCAAAGATTTTTAGGGTTTAATTTAGGCATAAACGGATCCATAAGATAACGACATCAAAAGCACCGCAGCTTTTTCTTCTTCTCCCAATTTCTTCCAATGCGATCTCTGCATAAACACTTCTTTCCCCAAATTCAATAGTCTCTGCTTCATGCTCAAATTATCCCCAACCTTGCTTCCACCGCTTCCATTGCTCTTTTTCCCCTTTCTCGCTCCGTCTTCGTTGTTTCCTTTGCTTATCCCCAAAATCGCTCTCTTCTTCTTCCTGCTCCTGATCCCGCAAGCATTACACAGAGACTTTGGTCCTGCAGGTCCGCCTCTCCATAGAGGAGTTTTGGAAGTTCCGCAATCGGCGCAGGTTTTCTTCGGACTGTTTTCCGATGAAACGGCGTTAGGGTTTGAATCTTCACCTTCTGATCCCTTTCCAGTATGATCAACCACCATTCTGATTCGATTCTGATCAAACACAAACGCGATTCCGATGACTGAAACAGAATTGGATGAATCAGAATGGAAGATCGAGCATGCAAGATTGAGAAAATAATGGTGATGATGATGATGATGAAGTGACTGTTTAGTAGAGAGAGAGAGGAGGGGAGAGATTTATAGAGAGAGAGAGATTGGGAGAGATAGGAATCGTAAAGAGACACTGATATACTCCTAGAGACACACGCACGCACCAGCCGCCCCGCGTACTCTGTCGTTGACTACCACTGTCTT
TARGET=837,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06204 Ps_contig_mira-and-tgicl-ass_franssen_42350
SEQUENCE=AAGAGACCAAATTGTGGTCCATTCCACGAAATACCACTCTTCTTTTTTTTGTTCTATCAATCCGGATTCCGAACGAATCCTACAATAGTTAATCTAAAATCCTTGCCGTATTTTCCACATCTCACCAAAATCTTTACGCTGATCCAAGTGAAAGATGGATTCCCTTGATCAAATGATGGAAGATGGTGCTACTTATTCTCAGGCTGAACCACAAAAACCAGTATGGTGTGAGAGAAAACAAGCCGTAGTACAAGAAGAAATATGGAGAATGAATCAATTACCTGCAAAAAGTACCTATGTTGCACATCGTCTAAGGGTTCTTAATAAAATTCTGCAACTTATGTCAGTTCAGAGAACTGTATCTCAAGAGAAGGAGTTGGAGTTGCTTTTTGCTGGTCTGTCATTGTGAAAGAATCATAAAATGCAGGGGATTAGCAGAAGCGTATTAGCAGAAGCGTATTAGCGTACTTCCAATAGTATATTTTCCGGAATTTCGATAGTACTATATTTTAACATATTTGAGAATATAGAATGTGGATCGAGAAACTACCTATGTATATTAAAAGGAGATTCGCGTTGAAGTTATTAGTATTTTCAAGCACCATTGGTGTTTGTTTTGTACNTTCTTTCATATCTATATCTATATATATATATTGTTGTTAAGTT
TARGET=642,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06205 Ps_contig_mira-and-tgicl-ass_franssen_42352
SEQUENCE=AAGGTTTAAACAAGTTACATACATACATTCGTTCRAGAAAGGCATTTTGAATTACAAGTCATTCAAGTTACATTACACCAAAATACATATCACAAAATTCCATGCAAGTTATATATTTTTGACCTACAATTGCCTCTAGTTAACTGTTGACTTTTTGGTCAAACAGTTGGCCAAAGACAATTGATCTAATTCTAAACCATATCCTATCTTCAATTGCTTCATGTTGTCCAAGATTCCTCATGATCCATATGATGCCTTGTCATCCAATTCCATGCCATATATGCCTACCTATGATTGAATCACAAAATTGCCCCATTAACTTGAAGGAAGCAGTTTCTAGTGTCATAGCTACAACTCCAAGATGGTCAGACACACATGAGCTCGAGGATGTGAAGAAGCATGTGGCGTCAAAGTATGGAAAAGAATGTGTCTCTTCAGTTGTTGTATTGCGCCCTGGTTGTGGTGTAAATCATTTGTTTGGTTGAGAAATTGTCTGCTAAAGCCCTCTGAAGTCTGGAATTCCCATCATCTTCTCCATTGCAGAGATTACCGGGACAAGACGCTTTCTCGTAGGTAGGCTTATCCTCATCCTTTTGCGTTACTCCAAGTCGCCACCACTTGAGGTTTGATTTGAGGAATCAAAGCCCTAACAGGATACACTTTGATTCCTTTATTGTTGCGTTTAATTTTCTAATTCATGACTTAGGGTTCATCCTTAGTTTGGTTGGATTTAAAGGTTTGAGTGGAAATTGATATAAAAACAAGCTTGGATAGTGATAGGAAAGGATTAGACGATCAAGATGGTATAAGGTTAGTGGGGTTTCGCCCTCCGCCACCGCCAGAGGCAATTTCTGGTGTGGAGGAGGTAGTATTCTTAGGCCATGTGATTTGTGAGAAGAATAAGTGATCTGAGCACGTGGACTAAGGTAAGTGTGATGAACCCTCTCCTCCT
TARGET=16,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06206 Ps_contig_mira-and-tgicl-ass_franssen_42361
SEQUENCE=TTAGTAAACNATTTAGTTGTATYARTGATGATAGAARATAATTACAAATGATACAGTTAAGTACAATTAACTAACTAATAACAAACACCAATAATAACTAACCCACTATATTTCCAATACTAGATACACAAATTCCAACTTCATTTTGTCTATATATCATGATATCATGATTGGAAGTGTTGTGGTCTAGTATATTTACATAACCTGGGTTTATATTAAAACTTTTCCTGCACTCTACAACATTCTTGAATGAAACTAGCTAAACTCTACACATTCTTTCATCAATGACGCAGACCAAAATACCTTGACAATTAGAAGGATGAGGTTCCTTCAGCTTTTATCGCCTTCAGAGTTGAAGCCAACCAGTCCAAGCCCTCGTAAAGGCCATCTCCCTTGAGGGCACAGGTGCCTTGTATGTGCCATTTCCTATTCTTTAGATCAAAGAGACCTAGTCCTTCACATACTTCCATTGGCGTCATCGCTCCTCTCAGGTCCTGTTTGTTTGCAAACACCAATATGACACTATTAAGCATAAAAGGGTCATTTATGATTGCCTGAAACTCCAGCTTTGCTTGACCTATTCTCTCACGATCGAGGCTATCAATAACATAAATCAGACCATCGGTGTTGTTAAAATAATGTCTCCAAAGTGGCCTTAATTTCTCTTGTCCTCCAACATCCCAAACCGTAAAAACAACATTCTTATACTGAACTTTCTCGACATTGAAACCAATAGTGGGAACAGTAGATAAAACTTCTCCAATGTGAAGTTTGTAGAGTATAGTAGTTTTTCCAGCAGCATCAAGACCAAGCATCACAACCCGCATATCAGAGTTGCCAAAGAAGGTGTCAAAGAGCTTCCGAAAAACTTGACCCATGGAAGCTTTCTGTGTTGAGAATCAGAGAGAGAGAGAGAGATTGTAATTAGTAAGTTTAGGGTAGGTTTAGTAATTTACAAAGAAAAGAACAACCACCCAAACGG
TARGET=902,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06207 Ps_contig_mira-and-tgicl-ass_franssen_42370
SEQUENCE=TTTATGTGTTCTGACTCAATTTTCAGATTGTTTCGGTGAGCATTTGCGGTTTGAATTATGTTTCTGATTCTTCTCCTTCTCCACTGTGATGTTGTGTTGTTGCTATTTGTTGTTTTTGGAACTTTGTCTTGTGGATTGGTTACCGCATTTGACGTTCTGTAACTCGCATACCAAGTGTTCGGTAAATTGCTTGAACCAAATTTTTTTCCTTTTTTTTATTTGTTTTGGTTCTGTTTGGGATTCTTTTTCTCACTGTTTTGGATCCTGAATCGATTTCTCTAATGGTTATGATATTTTAATTTGGTATTTATAAGAGTTGCGATAAAGATTCTTGGTGATGTTTCTTGTGGTCTATGGTGAACTAATGATGAAGGTTGGAAATAGTGAGGAAAGAAGTACAAGTTTTGGTATGAGTAAGATGCTGAATTTTCTATGATTTGTGTTCATGGCAAGGTTTAGATTCTCACCGTAATTGTCCCCTAAAGATGAAAAGTAAGGCAAATACATAAACAAGCATGAAAAGAATAAACCCAAACAAGCAAGTAAGCATAAAAGACATGAGCAAGCAAGCATTCATGGAGTGATAAATAAAGTTTGAGGCTTACCGATCGGAGACCCTCCGATGGTTCTTCAATGGCGGAACAAACTCAATACTG
TARGET=561,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1340
PRIMER_LEFT_PENALTY=0.032347
PRIMER_RIGHT_PENALTY=0.101680
PRIMER_LEFT_SEQUENCE=TTGTGTTCATGGCAAGGTTTAG
PRIMER_RIGHT_SEQUENCE=AGTATTGAGTTTGTTCCGCCAT
PRIMER_LEFT=437,22
PRIMER_RIGHT=654,22
PRIMER_LEFT_TM=60.032
PRIMER_RIGHT_TM=59.898
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=218
PRIMER_PAIR_PENALTY_1=0.1635
PRIMER_LEFT_1_PENALTY=0.061818
PRIMER_RIGHT_1_PENALTY=0.101680
PRIMER_LEFT_1_SEQUENCE=ACCCAAACAAGCAAGTAAGCAT
PRIMER_RIGHT_1_SEQUENCE=AGTATTGAGTTTGTTCCGCCAT
PRIMER_LEFT_1=528,22
PRIMER_RIGHT_1=654,22
PRIMER_LEFT_1_TM=60.062
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=0.2763
PRIMER_LEFT_2_PENALTY=0.032347
PRIMER_RIGHT_2_PENALTY=0.243965
PRIMER_LEFT_2_SEQUENCE=TTGTGTTCATGGCAAGGTTTAG
PRIMER_RIGHT_2_SEQUENCE=GTATTGAGTTTGTTCCGCCATT
PRIMER_LEFT_2=437,22
PRIMER_RIGHT_2=653,22
PRIMER_LEFT_2_TM=60.032
PRIMER_RIGHT_2_TM=60.244
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=217
PRIMER_PAIR_PENALTY_3=0.3058
PRIMER_LEFT_3_PENALTY=0.061818
PRIMER_RIGHT_3_PENALTY=0.243965
PRIMER_LEFT_3_SEQUENCE=ACCCAAACAAGCAAGTAAGCAT
PRIMER_RIGHT_3_SEQUENCE=GTATTGAGTTTGTTCCGCCATT
PRIMER_LEFT_3=528,22
PRIMER_RIGHT_3=653,22
PRIMER_LEFT_3_TM=60.062
PRIMER_RIGHT_3_TM=60.244
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=126
PRIMER_PAIR_PENALTY_4=0.3312
PRIMER_LEFT_4_PENALTY=0.229480
PRIMER_RIGHT_4_PENALTY=0.101680
PRIMER_LEFT_4_SEQUENCE=CACCGTAATTGTCCCCTAAAGA
PRIMER_RIGHT_4_SEQUENCE=AGTATTGAGTTTGTTCCGCCAT
PRIMER_LEFT_4=464,22
PRIMER_RIGHT_4=654,22
PRIMER_LEFT_4_TM=60.229
PRIMER_RIGHT_4_TM=59.898
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_06208 Ps_contig_mira-and-tgicl-ass_franssen_42388
SEQUENCE=GTTTAAATTAACCTTAATTACCGGTAAAAGGAGTCCCTCGTTTGGGTTGCTCAGATGAAGCGTGTTATTGATCTGTTGCTTGAGACGCGGAAGGCTTATACTCCAGAGCAAATAAATGAAGCATGCTATGTTGACATGAGGGCTAACAAAGATGTTTTTGACAATCTGAGGAAAAACCCAAAAGTACACTATGATGGGCAGCGATTTTCTTACAAGGCAAAGTATGGCCTTAAGGATAAAACTGAGCTTCTTCAACTGGTACGTAAGTATCCAGAGGGCATTGCTGTTATTGACTTAAAGGATGCTTACCTCACTGTGATGGAAGACTTACAGGCTTTGAAAGCTGCAGGGCAGATTTGGCTGCTTGCCAACTTCGATTCACAGGAAGACGTCGCATACCCGAATGACCCCAAAGCAAACATTAAGGTGGATGATGACCTAAAACAGCTATTCCGGAGTATTGAGTTGCCTCATGATATGATTGATATAGAGAAGGATCTTCAAAAGAATGGAATGAAGCCTGCTACCAACACTGCAAAGAGGAGGAGTGCAGCCCAAATTGATGGCATTTCTTCCAAGCCCAAGCCTAAGAAGAAGAAGAATGAAATCACCAAGAGAACCAAGCTGACCAATGCCCATCTTCCGGAGCTTTTTCGGAACCTAAACAATTCCTAATCAACCACTGCAAGTCTGCTAGTGCCGCATTAGATCAAGTAGAGAATTTGTTAGCCTAAGTTAGATCCGCAGGTTGAAAAATATTCAGGAGTTCTTATTGCTCTCTGCCCTATTCTTGATATATTGTTTTTTAATTTTNNNNTACTATCAAGG
TARGET=589,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1136
PRIMER_LEFT_PENALTY=0.110004
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=CTGCTACCAACACTGCAAAGAG
PRIMER_RIGHT_SEQUENCE=TGCAGTGGTTGATTAGGAATTG
PRIMER_LEFT=520,22
PRIMER_RIGHT=686,22
PRIMER_LEFT_TM=60.110
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=8.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=167
PRIMER_PAIR_PENALTY_1=0.1959
PRIMER_LEFT_1_PENALTY=0.192310
PRIMER_RIGHT_1_PENALTY=0.003589
PRIMER_LEFT_1_SEQUENCE=AATGAAGCCTGCTACCAACACT
PRIMER_RIGHT_1_SEQUENCE=TGCAGTGGTTGATTAGGAATTG
PRIMER_LEFT_1=512,22
PRIMER_RIGHT_1=686,22
PRIMER_LEFT_1_TM=60.192
PRIMER_RIGHT_1_TM=59.996
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.1000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=175
PRIMER_PAIR_PENALTY_2=0.2266
PRIMER_LEFT_2_PENALTY=0.110004
PRIMER_RIGHT_2_PENALTY=0.116582
PRIMER_LEFT_2_SEQUENCE=CTGCTACCAACACTGCAAAGAG
PRIMER_RIGHT_2_SEQUENCE=GCTTGGTTCTCTTGGTGATTTC
PRIMER_LEFT_2=520,22
PRIMER_RIGHT_2=624,22
PRIMER_LEFT_2_TM=60.110
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=105
PRIMER_PAIR_PENALTY_3=0.2276
PRIMER_LEFT_3_PENALTY=0.224014
PRIMER_RIGHT_3_PENALTY=0.003589
PRIMER_LEFT_3_SEQUENCE=AAGAATGGAATGAAGCCTGCTA
PRIMER_RIGHT_3_SEQUENCE=TGCAGTGGTTGATTAGGAATTG
PRIMER_LEFT_3=504,22
PRIMER_RIGHT_3=686,22
PRIMER_LEFT_3_TM=60.224
PRIMER_RIGHT_3_TM=59.996
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.2326
PRIMER_LEFT_4_PENALTY=0.110004
PRIMER_RIGHT_4_PENALTY=0.122569
PRIMER_LEFT_4_SEQUENCE=CTGCTACCAACACTGCAAAGAG
PRIMER_RIGHT_4_SEQUENCE=CAAGAATAGGGCAGAGAGCAAT
PRIMER_LEFT_4=520,22
PRIMER_RIGHT_4=792,22
PRIMER_LEFT_4_TM=60.110
PRIMER_RIGHT_4_TM=59.877
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=273
=
PRIMER_SEQUENCE_ID=SSR_06209 Ps_contig_mira-and-tgicl-ass_franssen_42389
SEQUENCE=ATGGTGCTCATGATAATGCATTAGTCGTCCCCCTTTCAGGAAAATCAACGGTTGATCTAATTAGAGTGACGAGTGAGTTCATTAACCCTTGTAGCTTTAGGGGCAGTTGTCAGTGATATAGGCTCATTTATCAGCATGAACGACAGGATAGCAGGGTCACCCCCAGGCAATGGATCCAGAGATGCTGCTGGTGAAGATTTGGTTGCAAGCTAGGAATTTTATCACTCAAGATGGTACAATGGAACCGGGAGGATAAGATGCTCGGACAAAAGCTACAAATCTGTCAAGCCTATGACCAAAATTCGTAACCCAAGCACGCCAACACGATATTCGTTTCACCGTCGTCGTCGTCGTC
TARGET=340,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06210 Ps_contig_mira-and-tgicl-ass_franssen_42394
SEQUENCE=TATCCTTCACTCATCACATAGAAAACTAGAACTCACAATTTCTTCAACCTTCACAAACACACACACAAAAAGAGACAAGTGAGAGAGAAAGAAAGAGAGGGACAAACATAACATAAACACTAGTGAGTAAGAGGTGATGGGAAATTACAGGTTCAAATTGTCTGATATGATGCCTAATGCTTGGTTTTACAAACTAAGAGACATGGAAAAAACAAGAAACCAAACACCAACATCTCAACAACATAGGAAGAAAAAACAAT
TARGET=57,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06211 Ps_contig_mira-and-tgicl-ass_franssen_42406
SEQUENCE=AAAGAAGAGTACGTTTGTTTACAAAAGTAAGAAGAATAGTCGGAGGAGGAGGAGGAGGAGGAGGAGAAAGACTCGTCACAACAGGATTTGTCTGTNTTGGATTTGCCTGAGCTTGTGTTGGATAGCATTCTTGAAAGGCTACCTCCGTCTTCGCTTTGTCAAATGACTGGCGTTTGTCATTCCTTGAGGGAGAGATGTGTGAGTGATCACCTTTGGGAGAGACATATGAAACAGAAATGGGGTAGAGTTATTGGTTCTGTTGCTTATAGGGAGTGGAAATGGCATGTTGCTTCTAAAAAGGATATTGGAAGCCTCACACATGGCAATCAAAGGAGCTTGATCAAGTTTATGTCCCTTCGTTGGCCTTTTACATGGATGAGAATCAAGCTAGATGCAGCATATATTAACAGTGCTAAGAAGCAGAATAGTTATTTGCCTCCTGATTCATTCATGACTTGGTATCTTGCTCTTGAGAATGGAAATTTCTCTTTTCCTGCTCAAGTCTATAACCGTGAGAAT
TARGET=42,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06212 Ps_contig_mira-and-tgicl-ass_franssen_42429
SEQUENCE=AAACAAACGCTAACCCATCTCTTTCTCAACCGCCGTTGCCGGTGGTTTCTTTCTCCTTTTCGGTTCAACGCACTCCATCTCTTTCGAGCTTTTAGGGTGGTTTAGGCACAGATCTGGAAACGGAGTTGATGGCGACATTACCGGTCGAGCAAACTGGTGAATCTCCGACGACGGAATTGCAGAGATCCATTCCGACGCCGTTTCTTACCAAAACGTATCAACTTGTGGATGATCCTTCTGTTGATGATTTGATTTCATGGAACGAAGACGGGACAAGCTTCATAGTTTGGTTACCTGCTGAATTCGCAAGAGATTTGCTACCGAAGTATTTCAAACACAATAACTTCTCCAGTTTTGTTCGTCAGCTTAACACATATGGATTTCGGAAAGTTGTACCTGACCGTTGGGAATTTGCCAACGACGGTTTCCGACGAGGCGAGAAAAATCTACTCCGTGATATACAACGGAGGAAAATATTGTCGGCTGCCGGAACGCCGATTGTGACGACGGTAGCCGCGGCAACTAATGTTGTAACGGTAGCTATGGCGGCACCGGTTAGAATGGTGTCTCCAACAACTTCCGGTGATGAACAGGTGGTTTCATCGAACTCATCTCCAATTGTGGCGCACAATAGTGCCACAGTGCACCGTAGCACAAGTTGCACCACTTCGCCTGAATTATTTGAAGAGAATGAGAGGCTTAAGAAAGAGAATACTCAACTGAGTAACGAGTTGAGTCAATTGAAAAGTCTTTGTAATAGTATATTATCTATGATGAGTAATTATGCTTCTGGTTTTAGTCGACAGTTAGAATCGTCTACTAGCGTTGCAACGGCACGGGCGGTGCTTGTAAATGAAGGAGGCAAAGCTTTGGATCTTTTGCCTACAAGGAATGTTTCGTTAGCTGAGGAGGCTGTGGCTGTTAATGTAGGTGGTGTTGCCGGTGGTGGTGGTGCATCCTGTGAGACGCTGAATTTGGCTGGTAATGCCTGTGGTGCATCATGTGAGACGCAGAATTTGGCTGAACCTGAGCATGGGCATGAGGCTCAAGTTCCTAAACTGTTTGGGGTGTCTATTGGGCTTAAACGATGTAGGAGAGAGTGTGAG
TARGET=942,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3454
PRIMER_LEFT_PENALTY=0.209271
PRIMER_RIGHT_PENALTY=0.136083
PRIMER_LEFT_SEQUENCE=TGTTAATGTAGGTGGTGTTGCC
PRIMER_RIGHT_SEQUENCE=TCCTACATCGTTTAAGCCCAAT
PRIMER_LEFT=919,22
PRIMER_RIGHT=1094,22
PRIMER_LEFT_TM=59.791
PRIMER_RIGHT_TM=59.864
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=176
PRIMER_PAIR_PENALTY_1=0.3490
PRIMER_LEFT_1_PENALTY=0.209271
PRIMER_RIGHT_1_PENALTY=0.139753
PRIMER_LEFT_1_SEQUENCE=TGTTAATGTAGGTGGTGTTGCC
PRIMER_RIGHT_1_SEQUENCE=CAAATTCTGCGTCTCACATGAT
PRIMER_LEFT_1=919,22
PRIMER_RIGHT_1=1018,22
PRIMER_LEFT_1_TM=59.791
PRIMER_RIGHT_1_TM=60.140
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=100
PRIMER_PAIR_PENALTY_2=0.3527
PRIMER_LEFT_2_PENALTY=0.216588
PRIMER_RIGHT_2_PENALTY=0.136083
PRIMER_LEFT_2_SEQUENCE=AATGTTTCGTTAGCTGAGGAGG
PRIMER_RIGHT_2_SEQUENCE=TCCTACATCGTTTAAGCCCAAT
PRIMER_LEFT_2=890,22
PRIMER_RIGHT_2=1094,22
PRIMER_LEFT_2_TM=59.783
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=205
PRIMER_PAIR_PENALTY_3=0.3563
PRIMER_LEFT_3_PENALTY=0.216588
PRIMER_RIGHT_3_PENALTY=0.139753
PRIMER_LEFT_3_SEQUENCE=AATGTTTCGTTAGCTGAGGAGG
PRIMER_RIGHT_3_SEQUENCE=CAAATTCTGCGTCTCACATGAT
PRIMER_LEFT_3=890,22
PRIMER_RIGHT_3=1018,22
PRIMER_LEFT_3_TM=59.783
PRIMER_RIGHT_3_TM=60.140
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=6.5000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=129
PRIMER_PAIR_PENALTY_4=0.3926
PRIMER_LEFT_4_PENALTY=0.256503
PRIMER_RIGHT_4_PENALTY=0.136083
PRIMER_LEFT_4_SEQUENCE=AGCTTTGGATCTTTTGCCTACA
PRIMER_RIGHT_4_SEQUENCE=TCCTACATCGTTTAAGCCCAAT
PRIMER_LEFT_4=865,22
PRIMER_RIGHT_4=1094,22
PRIMER_LEFT_4_TM=60.257
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=230
=
PRIMER_SEQUENCE_ID=SSR_06213 Ps_contig_mira-and-tgicl-ass_franssen_42436
SEQUENCE=AAGGCAGNGGTTCAACGCAGAGTACTCGGGGAGTCATTTATTCGATTCAAATAATTGATTTTCGCTTTGTACGGGGTAGAGTCGATGGCAGAATTCAGAAACAAAAACAACACCCATACGTCGTCGTCTAGGGTTCCGATCTTCAACCGTTCTTTGACAACGATTCACTCAGAAGAAAAACAAGCTTTACAAAAAACCCAAAACCTCGAACGCGTCGCCTCGCTTCGCGGCAGGGTCGTCAAAAAGCTCTGTAGTTTGTTCGAACCGGAAACACCGTCTTCACAAGAAGAATCAACACCTTTCTCCAACAAACCAAAACCCTTGAAATCAACCGAATCAGCATCGGTTTCAAAATCGTTGGATGCTTCACGGATCATTCGATTACCGGGAACAGAAGATCGAATCGTGTTGTATTTCACGAGTTTGCGTGGAATTAGACGAACGTATGAAGATTGCTATGCTGTTAGGTTGATATTGAGAGGATTTAGGGTTTGGGTTGACGAAAGAGATGTTTCGATGGATTCTTGTTACAGGAAGGAACTGATGAATGTGTTGGGTGAGAAGAGTATGAAGAATGTTACCCTTCCTCAGGTTTTTATTCCGAGGGAATCTCGTTGGTGGTGCTGAAGTGATGAAGCAGCTTTGTGAGATTGGTGAACTGGGGAAGATCTTGGAGGGTTTTCCGAAGACGAAAGCTGGGTTTGTTTGTGAGAGTTGTGGTGATATTAGGTTTATGCCTTGTGGGAATTGTAGTGGAAGTAGGAAGGTTTTTGATGAGGATGAAGAGTTGTTGAAGAGGTGTTTGGAGTGTAATGAAAATGGATTGATTCGGTGTCCGAATTGTTGCAGTGCTTAAGATTCTTTTCGACGCGTTCATTAGTAGTTCGTGTCTTTGCAATTGCAGCTTGTTTATTGTTGCTTGCTTGCAAGTTTTTAGGGTTTGATATATATATAAATTTGATCAAGATCGATTCATTTTTCAGTTGTTGGTGGTGTCGACAGGGGTTTATATATAAATAAAGGTATGCAATGTGATTCTAGACGCGACCACATTTAGCCATCATGGA
TARGET=916,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3494
PRIMER_LEFT_PENALTY=0.263023
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=TAGGTTTATGCCTTGTGGGAAT
PRIMER_RIGHT_SEQUENCE=GACACCACCAACAACTGAAAAA
PRIMER_LEFT=726,22
PRIMER_RIGHT=996,22
PRIMER_LEFT_TM=59.737
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.3854
PRIMER_LEFT_1_PENALTY=0.299089
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=GGAAGGTTTTTGATGAGGATGA
PRIMER_RIGHT_1_SEQUENCE=GACACCACCAACAACTGAAAAA
PRIMER_LEFT_1=761,22
PRIMER_RIGHT_1=996,22
PRIMER_LEFT_1_TM=60.299
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.4126
PRIMER_LEFT_2_PENALTY=0.115769
PRIMER_RIGHT_2_PENALTY=0.296849
PRIMER_LEFT_2_SEQUENCE=GTTTATGCCTTGTGGGAATTGT
PRIMER_RIGHT_2_SEQUENCE=CACCACCAACAACTGAAAAATG
PRIMER_LEFT_2=729,22
PRIMER_RIGHT_2=994,22
PRIMER_LEFT_2_TM=60.116
PRIMER_RIGHT_2_TM=60.297
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.4821
PRIMER_LEFT_3_PENALTY=0.185267
PRIMER_RIGHT_3_PENALTY=0.296849
PRIMER_LEFT_3_SEQUENCE=TGTTGAAGAGGTGTTTGGAGTG
PRIMER_RIGHT_3_SEQUENCE=CACCACCAACAACTGAAAAATG
PRIMER_LEFT_3=788,22
PRIMER_RIGHT_3=994,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=60.297
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=0.5599
PRIMER_LEFT_4_PENALTY=0.263023
PRIMER_RIGHT_4_PENALTY=0.296849
PRIMER_LEFT_4_SEQUENCE=ATTAGGTTTATGCCTTGTGGGA
PRIMER_RIGHT_4_SEQUENCE=CACCACCAACAACTGAAAAATG
PRIMER_LEFT_4=724,22
PRIMER_RIGHT_4=994,22
PRIMER_LEFT_4_TM=59.737
PRIMER_RIGHT_4_TM=60.297
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=271
=
PRIMER_SEQUENCE_ID=SSR_06214 Ps_contig_mira-and-tgicl-ass_franssen_42436
SEQUENCE=AAGGCAGNGGTTCAACGCAGAGTACTCGGGGAGTCATTTATTCGATTCAAATAATTGATTTTCGCTTTGTACGGGGTAGAGTCGATGGCAGAATTCAGAAACAAAAACAACACCCATACGTCGTCGTCTAGGGTTCCGATCTTCAACCGTTCTTTGACAACGATTCACTCAGAAGAAAAACAAGCTTTACAAAAAACCCAAAACCTCGAACGCGTCGCCTCGCTTCGCGGCAGGGTCGTCAAAAAGCTCTGTAGTTTGTTCGAACCGGAAACACCGTCTTCACAAGAAGAATCAACACCTTTCTCCAACAAACCAAAACCCTTGAAATCAACCGAATCAGCATCGGTTTCAAAATCGTTGGATGCTTCACGGATCATTCGATTACCGGGAACAGAAGATCGAATCGTGTTGTATTTCACGAGTTTGCGTGGAATTAGACGAACGTATGAAGATTGCTATGCTGTTAGGTTGATATTGAGAGGATTTAGGGTTTGGGTTGACGAAAGAGATGTTTCGATGGATTCTTGTTACAGGAAGGAACTGATGAATGTGTTGGGTGAGAAGAGTATGAAGAATGTTACCCTTCCTCAGGTTTTTATTCCGAGGGAATCTCGTTGGTGGTGCTGAAGTGATGAAGCAGCTTTGTGAGATTGGTGAACTGGGGAAGATCTTGGAGGGTTTTCCGAAGACGAAAGCTGGGTTTGTTTGTGAGAGTTGTGGTGATATTAGGTTTATGCCTTGTGGGAATTGTAGTGGAAGTAGGAAGGTTTTTGATGAGGATGAAGAGTTGTTGAAGAGGTGTTTGGAGTGTAATGAAAATGGATTGATTCGGTGTCCGAATTGTTGCAGTGCTTAAGATTCTTTTCGACGCGTTCATTAGTAGTTCGTGTCTTTGCAATTGCAGCTTGTTTATTGTTGCTTGCTTGCAAGTTTTTAGGGTTTGATATATATATAAATTTGATCAAGATCGATTCATTTTTCAGTTGTTGGTGGTGTCGACAGGGGTTTATATATAAATAAAGGTATGCAATGTGATTCTAGACGCGACCACATTTAGCCATCATGGA
TARGET=944,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3494
PRIMER_LEFT_PENALTY=0.263023
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=TAGGTTTATGCCTTGTGGGAAT
PRIMER_RIGHT_SEQUENCE=GACACCACCAACAACTGAAAAA
PRIMER_LEFT=726,22
PRIMER_RIGHT=996,22
PRIMER_LEFT_TM=59.737
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=271
PRIMER_PAIR_PENALTY_1=0.3854
PRIMER_LEFT_1_PENALTY=0.299089
PRIMER_RIGHT_1_PENALTY=0.086339
PRIMER_LEFT_1_SEQUENCE=GGAAGGTTTTTGATGAGGATGA
PRIMER_RIGHT_1_SEQUENCE=GACACCACCAACAACTGAAAAA
PRIMER_LEFT_1=761,22
PRIMER_RIGHT_1=996,22
PRIMER_LEFT_1_TM=60.299
PRIMER_RIGHT_1_TM=59.914
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=236
PRIMER_PAIR_PENALTY_2=0.4126
PRIMER_LEFT_2_PENALTY=0.115769
PRIMER_RIGHT_2_PENALTY=0.296849
PRIMER_LEFT_2_SEQUENCE=GTTTATGCCTTGTGGGAATTGT
PRIMER_RIGHT_2_SEQUENCE=CACCACCAACAACTGAAAAATG
PRIMER_LEFT_2=729,22
PRIMER_RIGHT_2=994,22
PRIMER_LEFT_2_TM=60.116
PRIMER_RIGHT_2_TM=60.297
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=266
PRIMER_PAIR_PENALTY_3=0.4821
PRIMER_LEFT_3_PENALTY=0.185267
PRIMER_RIGHT_3_PENALTY=0.296849
PRIMER_LEFT_3_SEQUENCE=TGTTGAAGAGGTGTTTGGAGTG
PRIMER_RIGHT_3_SEQUENCE=CACCACCAACAACTGAAAAATG
PRIMER_LEFT_3=788,22
PRIMER_RIGHT_3=994,22
PRIMER_LEFT_3_TM=60.185
PRIMER_RIGHT_3_TM=60.297
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=207
PRIMER_PAIR_PENALTY_4=0.5304
PRIMER_LEFT_4_PENALTY=0.047652
PRIMER_RIGHT_4_PENALTY=0.482784
PRIMER_LEFT_4_SEQUENCE=GCAGCTTGTTTATTGTTGCTTG
PRIMER_RIGHT_4_SEQUENCE=ATATAAACCCCTGTCGACACCA
PRIMER_LEFT_4=900,22
PRIMER_RIGHT_4=1011,22
PRIMER_LEFT_4_TM=59.952
PRIMER_RIGHT_4_TM=60.483
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=112
=
PRIMER_SEQUENCE_ID=SSR_06215 Ps_contig_mira-and-tgicl-ass_franssen_42446
SEQUENCE=TATTGCTCTTATCTGTCACACTTGATGAACATTCATTCAAAAGCTAAAGTGAAATCACTTACTACCTTACAATTAAAACCGATTTTGGCGCTACACCAACCATTCACCAACTTCAATTGATCCTTACATTCCACGTTTTCATCATCATCATCATCATCATCACTTTCTTCATGTATTGCTTCTTCTTTCTGTGGTGAATTTTCCGATCTCATTTCCTCGTTACGTCGCTCGCCGAGCAATTTTGTTTACCGGAGATGACAGAGTTATGCTGGCCTTCTGATACAGAGCTAAATGAAATGAGAGAGAAAGTTTCTGAAATGGCAAATGTGAATAAAGAGGACGTTCGAGTTGTAGTATCTCCCTATCGAATTTGTCCTTTGGGGGCACACATTGATCATCAGGGTGGGACTGTTTTAGCTATGACAATCGATAAGGGAATACTTCTGGGGTTTACTCCTTCTGGAAGTGATGAGTTTGTACTTCGTTCGGGACAGTTTCAAGGAGAAGTTACGTTCAGAGTTGACGAGA
TARGET=139,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7484
PRIMER_LEFT_PENALTY=0.661698
PRIMER_RIGHT_PENALTY=0.086698
PRIMER_LEFT_SEQUENCE=CAATTGATCCTTACATTCCACG
PRIMER_RIGHT_SEQUENCE=AGAAGGCCAGCATAACTCTGTC
PRIMER_LEFT=113,22
PRIMER_RIGHT=277,22
PRIMER_LEFT_TM=59.338
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=165
PRIMER_PAIR_PENALTY_1=0.7638
PRIMER_LEFT_1_PENALTY=0.661698
PRIMER_RIGHT_1_PENALTY=0.102129
PRIMER_LEFT_1_SEQUENCE=CAATTGATCCTTACATTCCACG
PRIMER_RIGHT_1_SEQUENCE=CAGCATAACTCTGTCATCTCCG
PRIMER_LEFT_1=113,22
PRIMER_RIGHT_1=270,22
PRIMER_LEFT_1_TM=59.338
PRIMER_RIGHT_1_TM=59.898
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=158
PRIMER_PAIR_PENALTY_2=0.7683
PRIMER_LEFT_2_PENALTY=0.737989
PRIMER_RIGHT_2_PENALTY=0.030276
PRIMER_LEFT_2_SEQUENCE=TACACCAACCATTCACCAACTT
PRIMER_RIGHT_2_SEQUENCE=TTCACCACAGAAAGAAGAAGCA
PRIMER_LEFT_2=91,22
PRIMER_RIGHT_2=197,22
PRIMER_LEFT_2_TM=59.262
PRIMER_RIGHT_2_TM=60.030
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=107
PRIMER_PAIR_PENALTY_3=0.7683
PRIMER_LEFT_3_PENALTY=0.737989
PRIMER_RIGHT_3_PENALTY=0.030276
PRIMER_LEFT_3_SEQUENCE=TACACCAACCATTCACCAACTT
PRIMER_RIGHT_3_SEQUENCE=TCACCACAGAAAGAAGAAGCAA
PRIMER_LEFT_3=91,22
PRIMER_RIGHT_3=196,22
PRIMER_LEFT_3_TM=59.262
PRIMER_RIGHT_3_TM=60.030
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=106
PRIMER_PAIR_PENALTY_4=0.7946
PRIMER_LEFT_4_PENALTY=0.661698
PRIMER_RIGHT_4_PENALTY=0.132857
PRIMER_LEFT_4_SEQUENCE=CAATTGATCCTTACATTCCACG
PRIMER_RIGHT_4_SEQUENCE=TCTGTATCAGAAGGCCAGCATA
PRIMER_LEFT_4=113,22
PRIMER_RIGHT_4=285,22
PRIMER_LEFT_4_TM=59.338
PRIMER_RIGHT_4_TM=59.867
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=7.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=173
=
PRIMER_SEQUENCE_ID=SSR_06216 Ps_contig_mira-and-tgicl-ass_franssen_42447
SEQUENCE=TCGGGRTGAGGACTCTCTTTTCTTCCAACGGGAAGATAGGAAAATAGATAAAAAAGAACAGTTCTCTCTCTAAGTAGATACCACCAACGTCTCTCTGCTTTGAAGATCCCTTCTCTCTCCATTCACAAGAAGAACCAAAAAAACACACTCAAAGAAAACAAGCAAAGAAAAAGAGAGGACTACTATTCCTCTTCTCCACTACACTCGACGCACCCCTCAACTCACACCATTCACCATCAATAACCTAAACCTTCTTCCGCCATCGTCTCCACCCCCTTCTCACGCGCAATTCCATCTTTTTCCTTCGAAACCTCTCAACATTCCTCGTCGTCATTCACCCGCCACCACTCAACCCTCCATTACCGGCGAACCTTCTAACTCCGTCGAATCTCCACACCACCGCGCACCACCGCCGCGCATAAACCTATCGCCGCTGCGAACCACCGACTACACGCCGTTAACGTCACCCTCAACACCCTTCCTTTCCCCTACCTCCACGATCTAGTTTTCGACGCCGATACAACCTTCCGCCGTCAACCACTCGACCATTACCGGCGACGACATCTCCAACAACAACAACGCTGCCGACGAATTTCTTCAACGCCTTCCCCCTTCAGCTTCACCATCGTGGGATTTGGTCTGTGATGTCAAGATCTAGATGATTGTTGTTCAACTTCATTTTTCGGCATGGCGGTAATTGGTCAGAGAACGTGTGCGCGGTAGCAGGTAATCCTCGAAATTGTTTGATAGAATTTGTTTTTTCTTTATCTTCCAATTGTGGATTCTTTTCGATGTGGAATGTTGAACTGGTTTGTTAAAGTTTGCACCATTTTTATTAGTGGACTGATGTGTTAATCCTACAACATTCTACTGTTAATTTTGAACATGCCTGATAACTTTTACAGCATTAAAGCTAGTGATCCTAGACAGATGCTAATATTGGAGCAATTTCAATTTAAAATGAATATGATTTGCTTGAAATTAAAGGATGTATAAAAAGTTGGAAGAAGACGAAATATGTAGTGTCTTCATCGTTTTATTTCCAGTTCATGCATCTCCTAATTCCTAGCCTTTTCTTTGTTTCATGACTTTGGGTATTGAAGAAAAGAAAGGCAAACTGTCGTTTCACACTTGTTTGTGTTTGTACATTAATTCTTATTATATTAATTTTCATGTGATGTTATTGTTATTATTTCTAGCTTGTGGTTGTACATTAATTCTCATTATATTAATTTTCATGTGGTGTTATTATTATCATTTCTAGCAACGTACAGATCATGGCATTTGGATGAGAAGTTAGGCTCATTTTTGGTGTACCAATGTTTTATTACTTACTACCCCCATGCTATTAACTAATTCATGGTGGCAAATAGTTAAA
TARGET=568,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06217 Ps_contig_mira-and-tgicl-ass_franssen_42464
SEQUENCE=ACTGGTCTAAGAGAAAGTTTAGGTGGTGTCCAGAGATGGATACTATATTTCCATGGACAGTGTGCTATTATTAATGTTTGTATGTTACTGCACGGCTGACGTACAAGAATGTCCCTACTTGGCACCGTGATCTTTGCCGGGTCTGTGAAAACATCCCAATTGTTCTTTGTGGAAACAAGGTTGATGTGAAGAACAGGCAAGTGAAGGCAAAGCAGGTTACCTTCCACAGGAAGAAAAATTTGCAGTACTATGAAATATCAGCTAAGAGCAACTACAACTTTGAGAAGCCTTTCCTGTATCTTGCCAGGAAACTTGCAGGTGATCCTAATTTGCACTTTGTAGAATCTCCTGCACTGGCTCCACCAGAAGTTCAAATTGATATTGCTTTACAACAAAGGCATGAGAATGAGATTCTTGAGGCTGCTAATCAGCCCCTTCCTGATGATGATGACGATGCTTTTGAATAGGAAGGACCTTCGAGAGTGCTGGCCTATGTGTGTTGATTTGTGCCGTTATATGCTGTAATGTTATGTGTTTTGTGGTTGAACCGTTGACATTTTTTTGATATAGATTTATGGATTTCAATCATCATGTGATTGAGTTGTTTTACTTTTATCCAAACCCTCACTTAATGGCTGCTATGTATCTTTACTCTGCCGTGCCATTGAAAAAGGTGTTATTCGTTTAAAGGAAACACTGTTTCGTCATATGGTATAGAAATTCGAGTGAANCYCTRARAAANNA
TARGET=440,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06218 Ps_contig_mira-and-tgicl-ass_franssen_42467
SEQUENCE=GGGTAGGCATAGCGTATGCCAGCGGAAAGTAAGTGACGGGCTTAATAAAAGTAAGACATCTTAATAGCATCATGAATAAATTGAAAACCCCATATCTATATCCAAAAATGTTTAGTTCAAACTCAACACATAATTTTATAGAATGCACGAAAAGGCAACAGCTTAGGACAAAGGTCATTCATCGGAAGAGTCCTTCCTCTACTCAAATGCATCATCATCGTCATCAGGAAGGGGTTGATTAGCCGCTTCAATAATTTCATTCTCGTGCCTTTGTTGTAAGGCCAAATCAATTTGAACTTCTGGTGGAGCCAGTGCAGGAGATTCTACAAAGTGCAAATTAGGATCACCTGCAAGTTTCCTGGCAAGATACAGGAAAGGCTTCTCAAAGTTGTAGTTGCTCTTAGCTGATATCTCATAGTACTGCAAATTCTTCTTCCTGTGGAAAGTAACCTGCTTTGCCTTCACTTGCCTGTTCTTCACATCAACCTTGTTTCCGCAAAGAACAATTGGGATGTTTTCACAAACCCGACAAAGATCACGATGCCAGGTAGGAACATTTTTGTATGTCAGTCGGGCAGTAACATCAAACATTATAATAGCACACTGCCCGTGGATATAATATCCATCTCTAAGACCACCAAACTTCTCTTGACCAGCCGTATCCCAGCAGTAGAAACGAATCTTGCCACAGTTAGTGAAAAAATCCAATGGATGCACTTCAACACCAATAGTTGGTTCATATTTCTTCTCAAATTCTCCGGTAAGATGCCTCTTAACAAATGTCGTCTTTCCTGTTCCACCATCGCCGACGATAACAAGCTTGAAACTCGGGTAATCGACGGTTTGCTGATTCGGCAAAGCCATAGATAGAGTATTGTTGGAATGGAGAGGCGGTGTATGAGTGATGATGAGTAGTGAATAAACAAATTAGGGTTTTTATATTAGAAGGGTTTCGTTTCGTTTCGTTTTGAGTTTGAGTTTGAAAGCGTGAAACTGACGCNGCTCTTGCGCCTTTCGGTCAAGTTTTCAACTTGTCTC
TARGET=950,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4131
PRIMER_LEFT_PENALTY=0.082113
PRIMER_RIGHT_PENALTY=0.331013
PRIMER_LEFT_SEQUENCE=ATTCGGCAAAGCCATAGATAGA
PRIMER_RIGHT_SEQUENCE=AGTTGAAAACTTGACCGAAAGG
PRIMER_LEFT=849,22
PRIMER_RIGHT=1031,22
PRIMER_LEFT_TM=60.082
PRIMER_RIGHT_TM=59.669
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.4131
PRIMER_LEFT_1_PENALTY=0.082113
PRIMER_RIGHT_1_PENALTY=0.331013
PRIMER_LEFT_1_SEQUENCE=GATTCGGCAAAGCCATAGATAG
PRIMER_RIGHT_1_SEQUENCE=AGTTGAAAACTTGACCGAAAGG
PRIMER_LEFT_1=848,22
PRIMER_RIGHT_1=1031,22
PRIMER_LEFT_1_TM=60.082
PRIMER_RIGHT_1_TM=59.669
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.4265
PRIMER_LEFT_2_PENALTY=0.095445
PRIMER_RIGHT_2_PENALTY=0.331013
PRIMER_LEFT_2_SEQUENCE=CTCAAATTCTCCGGTAAGATGC
PRIMER_RIGHT_2_SEQUENCE=AGTTGAAAACTTGACCGAAAGG
PRIMER_LEFT_2=747,22
PRIMER_RIGHT_2=1031,22
PRIMER_LEFT_2_TM=60.095
PRIMER_RIGHT_2_TM=59.669
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=285
PRIMER_PAIR_PENALTY_3=0.4601
PRIMER_LEFT_3_PENALTY=0.129103
PRIMER_RIGHT_3_PENALTY=0.331013
PRIMER_LEFT_3_SEQUENCE=GATAACAAGCTTGAAACTCGGG
PRIMER_RIGHT_3_SEQUENCE=AGTTGAAAACTTGACCGAAAGG
PRIMER_LEFT_3=810,22
PRIMER_RIGHT_3=1031,22
PRIMER_LEFT_3_TM=60.129
PRIMER_RIGHT_3_TM=59.669
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=11.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=222
PRIMER_PAIR_PENALTY_4=0.4601
PRIMER_LEFT_4_PENALTY=0.129103
PRIMER_RIGHT_4_PENALTY=0.331013
PRIMER_LEFT_4_SEQUENCE=CAAGCTTGAAACTCGGGTAATC
PRIMER_RIGHT_4_SEQUENCE=AGTTGAAAACTTGACCGAAAGG
PRIMER_LEFT_4=815,22
PRIMER_RIGHT_4=1031,22
PRIMER_LEFT_4_TM=60.129
PRIMER_RIGHT_4_TM=59.669
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_06219 Ps_contig_mira-and-tgicl-ass_franssen_42477
SEQUENCE=GGNTGTTCTTAAGACTACCATGTATATAATTCCATTCAGAAACAACTAATTTACACAAAAGGACAACAGTTCTCAAATTTACAAGCCTGTTCCTACCAAAAAAAAAACTCAAACACAAATCAACATTTGGCTATTCGATCTACCAATAAAATAAGAGAAAGAGTAAGAAAGAGAGAGGAGGGAGAGAGAAACTATGTGGTGCCAAGCTTCAACTGCATCTTGAGAATATTATCTAATAAAGACAAACTCTTAACTACCCTCATAACACCATATTAAAGTTGTTAGTTCAAATCTCGGAATGATTTATCTGGCGGATGACGACTCGCCCAGAGAAACAGAAGCCAAAGATGAAGCCAAAACCCGCGCACTCCTTGCCCTCTCGTGAAAAATCCTCGTCTTGCATAGTCCTAGCTTTGTCCAAATCCTCCTGACATCAATCACTTGAAGCACACGTCCGATCACTTGGCTTGTCTCACCAAATATTTGCATGTTCAGATTAGGAATACAGGAAACCCAGAATGCTCGGAGACGCGATAGGATCTTGGGAAATACAAAAAGAAGTATCCTTAAACAGATAAACATCACAGAAAACAATAAGTAGGGTTCCCTCATGGATGTTTCTTTAAGTGAGAGACCAATCAATGACTTCAGACTTGAATTCTCGTCGCCTGTCATCATCATACTGCTTGTTTGACCGATGGCATTATTACTACTTGCTTCAAACCTTGTGTTTCTTTCATAAACTCCAATAGAGAGAGAAAATCATTTGGGCCACGATACCTCAACTTTGAGGTTTGGTTCACCAATAATATTGAAGGCAAGCTATGGATTCCATATTTTGAATATAAGCTTGGCAAAGCTGACGATTGCTCAAGAACAAAATGCTCTATTTGAGGAAACATGGAGCTAAGAGTTTCAAATTTAGGAAGCATCATGCGTGAAAATGGGCACCAGGAAGCATAGAAGAGTATAGAGACAGACTCGGTCTTCTTTTTGCCCGATAGAACTTCTTCTATGAAATTTCCATCCACCTGAAGCGGTGGATTGGATTGGATGTAGATTGGGCATTGAGATTGAAGTTGATAGAGAAACGAAGGAGGTGTAGGGATGCAGGCGGAGGAGGAGGAAGAAGAAGCCGATGCGGATTGAAGAAGGGAGAGAGCGGTTATGGAAAGGAAAAGGAGCGACGCGGCCATAGATGAAGGTGGAGGAGGAGGAGGGTTAGGATGGTAAATAAGAGAAGAAAGGGGAGGGTAAATCGGGAAACGAAATAAAAGAGAGAAAAGGGTTTTAGGTTCGGTGGTGTTGTTATGCGTTYGGTTGTGTTTGTGTGATGATGATTTCTTTTCACCTCCCCGNGTACTCTGCGTTAG
TARGET=1041,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06220 Ps_contig_mira-and-tgicl-ass_franssen_42477
SEQUENCE=GGNTGTTCTTAAGACTACCATGTATATAATTCCATTCAGAAACAACTAATTTACACAAAAGGACAACAGTTCTCAAATTTACAAGCCTGTTCCTACCAAAAAAAAAACTCAAACACAAATCAACATTTGGCTATTCGATCTACCAATAAAATAAGAGAAAGAGTAAGAAAGAGAGAGGAGGGAGAGAGAAACTATGTGGTGCCAAGCTTCAACTGCATCTTGAGAATATTATCTAATAAAGACAAACTCTTAACTACCCTCATAACACCATATTAAAGTTGTTAGTTCAAATCTCGGAATGATTTATCTGGCGGATGACGACTCGCCCAGAGAAACAGAAGCCAAAGATGAAGCCAAAACCCGCGCACTCCTTGCCCTCTCGTGAAAAATCCTCGTCTTGCATAGTCCTAGCTTTGTCCAAATCCTCCTGACATCAATCACTTGAAGCACACGTCCGATCACTTGGCTTGTCTCACCAAATATTTGCATGTTCAGATTAGGAATACAGGAAACCCAGAATGCTCGGAGACGCGATAGGATCTTGGGAAATACAAAAAGAAGTATCCTTAAACAGATAAACATCACAGAAAACAATAAGTAGGGTTCCCTCATGGATGTTTCTTTAAGTGAGAGACCAATCAATGACTTCAGACTTGAATTCTCGTCGCCTGTCATCATCATACTGCTTGTTTGACCGATGGCATTATTACTACTTGCTTCAAACCTTGTGTTTCTTTCATAAACTCCAATAGAGAGAGAAAATCATTTGGGCCACGATACCTCAACTTTGAGGTTTGGTTCACCAATAATATTGAAGGCAAGCTATGGATTCCATATTTTGAATATAAGCTTGGCAAAGCTGACGATTGCTCAAGAACAAAATGCTCTATTTGAGGAAACATGGAGCTAAGAGTTTCAAATTTAGGAAGCATCATGCGTGAAAATGGGCACCAGGAAGCATAGAAGAGTATAGAGACAGACTCGGTCTTCTTTTTGCCCGATAGAACTTCTTCTATGAAATTTCCATCCACCTGAAGCGGTGGATTGGATTGGATGTAGATTGGGCATTGAGATTGAAGTTGATAGAGAAACGAAGGAGGTGTAGGGATGCAGGCGGAGGAGGAGGAAGAAGAAGCCGATGCGGATTGAAGAAGGGAGAGAGCGGTTATGGAAAGGAAAAGGAGCGACGCGGCCATAGATGAAGGTGGAGGAGGAGGAGGGTTAGGATGGTAAATAAGAGAAGAAAGGGGAGGGTAAATCGGGAAACGAAATAAAAGAGAGAAAAGGGTTTTAGGTTCGGTGGTGTTGTTATGCGTTYGGTTGTGTTTGTGTGATGATGATTTCTTTTCACCTCCCCGNGTACTCTGCGTTAG
TARGET=1116,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06221 Ps_contig_mira-and-tgicl-ass_franssen_42477
SEQUENCE=GGNTGTTCTTAAGACTACCATGTATATAATTCCATTCAGAAACAACTAATTTACACAAAAGGACAACAGTTCTCAAATTTACAAGCCTGTTCCTACCAAAAAAAAAACTCAAACACAAATCAACATTTGGCTATTCGATCTACCAATAAAATAAGAGAAAGAGTAAGAAAGAGAGAGGAGGGAGAGAGAAACTATGTGGTGCCAAGCTTCAACTGCATCTTGAGAATATTATCTAATAAAGACAAACTCTTAACTACCCTCATAACACCATATTAAAGTTGTTAGTTCAAATCTCGGAATGATTTATCTGGCGGATGACGACTCGCCCAGAGAAACAGAAGCCAAAGATGAAGCCAAAACCCGCGCACTCCTTGCCCTCTCGTGAAAAATCCTCGTCTTGCATAGTCCTAGCTTTGTCCAAATCCTCCTGACATCAATCACTTGAAGCACACGTCCGATCACTTGGCTTGTCTCACCAAATATTTGCATGTTCAGATTAGGAATACAGGAAACCCAGAATGCTCGGAGACGCGATAGGATCTTGGGAAATACAAAAAGAAGTATCCTTAAACAGATAAACATCACAGAAAACAATAAGTAGGGTTCCCTCATGGATGTTTCTTTAAGTGAGAGACCAATCAATGACTTCAGACTTGAATTCTCGTCGCCTGTCATCATCATACTGCTTGTTTGACCGATGGCATTATTACTACTTGCTTCAAACCTTGTGTTTCTTTCATAAACTCCAATAGAGAGAGAAAATCATTTGGGCCACGATACCTCAACTTTGAGGTTTGGTTCACCAATAATATTGAAGGCAAGCTATGGATTCCATATTTTGAATATAAGCTTGGCAAAGCTGACGATTGCTCAAGAACAAAATGCTCTATTTGAGGAAACATGGAGCTAAGAGTTTCAAATTTAGGAAGCATCATGCGTGAAAATGGGCACCAGGAAGCATAGAAGAGTATAGAGACAGACTCGGTCTTCTTTTTGCCCGATAGAACTTCTTCTATGAAATTTCCATCCACCTGAAGCGGTGGATTGGATTGGATGTAGATTGGGCATTGAGATTGAAGTTGATAGAGAAACGAAGGAGGTGTAGGGATGCAGGCGGAGGAGGAGGAAGAAGAAGCCGATGCGGATTGAAGAAGGGAGAGAGCGGTTATGGAAAGGAAAAGGAGCGACGCGGCCATAGATGAAGGTGGAGGAGGAGGAGGGTTAGGATGGTAAATAAGAGAAGAAAGGGGAGGGTAAATCGGGAAACGAAATAAAAGAGAGAAAAGGGTTTTAGGTTCGGTGGTGTTGTTATGCGTTYGGTTGTGTTTGTGTGATGATGATTTCTTTTCACCTCCCCGNGTACTCTGCGTTAG
TARGET=1207,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06222 Ps_contig_mira-and-tgicl-ass_franssen_42479
SEQUENCE=AAGACATTAAATTAARARAAAAGTTTTGTAAGAAATTTGAGGGAAAAAAAAGAGTTTGGTAGTTTGGTTGCAGTGCATGGGAAGCAGAGTTTGATCTGTCTGGTGAAGAAAAGAATGAGTGAAGGTGGTGCTGCTGCTGCCAATGGTTCTTCTCCAAAGCTTTATGCTCACAAGCCCCGAAAAGCGCAACTGAAGCAATTTCGAGGGCAACACAAACCAAAGGAGTTCTCTTCGCCACCGGTGATGGGAACTCAGACGGCTACCCTCCCGCCGCCACCGCCATCGCCGCCCAAGGAATCCTTTATTCGGCGCTACAAGTTCCTTTGGCCTATGCTATTGGCTGTCAATCTCGGAGTCGGAGTTTATTTGTTTGCAAGAACAAACACAAAGAAGAAAGATGCTGCAGAGGAGGAAAAAGATGCAGCAAATCAAATTCCAGCTAATGGTACAACAAGTCTCGTTTCAGCTAATGGTACATCAACTCCCATTTCAGTTAAAGATGCTCTAGCTCTTCTTGCTGAAAGATCTGTCTCCCCACCATCTTATGCAAATCCCGCGATCAAACGAGAACCAATTCCGGAAGGCCAACAACGGGAACTCTTCAAGTGGATATTGGAAGAAAAGAGGAAGATCAAACCGAAAGATGAAGAGGAGAAAAAGAGAATTGAAGAAGAGAAAGCTCTACTTAAAAGCCTTATCCGGTCAAAATCTATCCCAAGTATCTAATGATGATGTTCTCCTATCTTTGTCCAAAGCGAAAAAAGAAATCAAGAATGTTTTTGAATTCGGATATACTTGAGTTGATTCCTTCAACGGTTCTCTTTTGGTTGCTTGGTTGGTTGATGAATTTTAGATTGTACATTTGTTTAGATTGTCATAATTTGTCATTTGAAAATTTGGGTGTGGCCCATTGTTTATTTCTTAGTAGACAGTTATACAAGAAATCAATGTCACTTGATGTACTATAATACATCTGTTCTGTTATAAGATATCATGTGCATTTTTACGT
TARGET=129,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06223 Ps_contig_mira-and-tgicl-ass_franssen_42480
SEQUENCE=TCGGGGCGCATTCAGTTCCTCACATAACACAAAATTTACACAAACTGGGGGGAAATCGAGAATCGAGAGCATAATCGAACATCGTTTCAGAAAGTGAAACAGAAGAAGAAGAACCAGAGAGAGAATAATCAAGGAAGGTAAAAATGACGGAAGCGATGATCAGGAAGAAGCCAGGAATGGCCAGCGTGAAGGACATGCCGGTTCTTCAGGACGGTCCACCTCCCGGCGGTTTCGCTCCCGTCCGGTTCGCTCGTCGAATCCCTAACACTGGTCCGAGTGCCATTGCCATTTTTCTCACCACGTTTGGTGCTTTCTCGTGGGGAATGTACCAGGTTGGACAGGGTAACAAGATCCGAAGGGCACTAAAGGAAGAGAAATATGCTGCCCGCAGAGCGATACTGCCTGTGCTACAGGCTGAAGAGGATGAGAGATTTGTTAAAGAGTGGCATAAGTATCTTGAGTACGAGGCAGATGTCATGAAGGATGTGCCTGGTTGGAAGGTCGGTGCAAGTGTCTATAATTCTGGAAGATGGGTGCCCCCAGCAAGCGGTGAGCTGCGTCCTGATGTCTGGTGATGGTATATTAGCCATCACTATCAAGAATTGGTCTGCTTCATACATATGTTACATTGTGATACTTTCATCCAGCACCTCTAAAATAAAGTTGTATCTCAAACATTTGAAATTGTGTTGTCGATTTCTAAACCAGGAATGTTGTTTTTTCTTTTCAATTTTTTCTTTAATGTTGGCTTATCTACTCCTCTCATAAGT
TARGET=101,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3886
PRIMER_LEFT_PENALTY=0.386267
PRIMER_RIGHT_PENALTY=0.002310
PRIMER_LEFT_SEQUENCE=GCATAATCGAACATCGTTTCAG
PRIMER_RIGHT_SEQUENCE=CTTCGGATCTTGTTACCCTGTC
PRIMER_LEFT=68,22
PRIMER_RIGHT=357,22
PRIMER_LEFT_TM=59.614
PRIMER_RIGHT_TM=59.998
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=290
PRIMER_PAIR_PENALTY_1=0.6513
PRIMER_LEFT_1_PENALTY=0.386267
PRIMER_RIGHT_1_PENALTY=0.265053
PRIMER_LEFT_1_SEQUENCE=GCATAATCGAACATCGTTTCAG
PRIMER_RIGHT_1_SEQUENCE=GATCTTGTTACCCTGTCCAACC
PRIMER_LEFT_1=68,22
PRIMER_RIGHT_1=352,22
PRIMER_LEFT_1_TM=59.614
PRIMER_RIGHT_1_TM=59.735
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=285
PRIMER_PAIR_PENALTY_2=0.6513
PRIMER_LEFT_2_PENALTY=0.386267
PRIMER_RIGHT_2_PENALTY=0.265053
PRIMER_LEFT_2_SEQUENCE=GCATAATCGAACATCGTTTCAG
PRIMER_RIGHT_2_SEQUENCE=GGATCTTGTTACCCTGTCCAAC
PRIMER_LEFT_2=68,22
PRIMER_RIGHT_2=353,22
PRIMER_LEFT_2_TM=59.614
PRIMER_RIGHT_2_TM=59.735
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=286
PRIMER_PAIR_PENALTY_3=0.6785
PRIMER_LEFT_3_PENALTY=0.676221
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=GAATCGAGAGCATAATCGAACA
PRIMER_RIGHT_3_SEQUENCE=CTTCGGATCTTGTTACCCTGTC
PRIMER_LEFT_3=59,22
PRIMER_RIGHT_3=357,22
PRIMER_LEFT_3_TM=59.324
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=299
PRIMER_PAIR_PENALTY_4=0.6992
PRIMER_LEFT_4_PENALTY=0.362762
PRIMER_RIGHT_4_PENALTY=0.336392
PRIMER_LEFT_4_SEQUENCE=CGCATTCAGTTCCTCACATAAC
PRIMER_RIGHT_4_SEQUENCE=TCCGTCATTTTTACCTTCCTTG
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=150,22
PRIMER_LEFT_4_TM=59.637
PRIMER_RIGHT_4_TM=60.336
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=145
=
PRIMER_SEQUENCE_ID=SSR_06224 Ps_contig_mira-and-tgicl-ass_franssen_42481
SEQUENCE=AAAGACAAGTAGGTAATACAAACGCAGAGTACGTCGGGGATTGCGTTTCGTTTCATCCATTTCCTTATCCTATTTTTTTCTCCATCTTCTCTCTTTCTCTCTCACTCTTTCAATTTTCCAACACAACAATGGCTTCAACAACCGCATTCACAACCATCTCAACCGTCACACGTTCGTATCCTACACCCTCCTCTTATCCTACACACTTCCCCAAACCAATCCCAACCGTCCATTTCCCATCCTCAACAACAACACCTCTCTCCCACCGCACCACTCGCCTCCACCGCATCACCGCCGTCTCCGCAGAAGTCGAAAAACTCGGCAACGAGATCTCCGGCCTCACACTCGAACAAGCCAAAAACCTAGTCGACTACCTCCAAGACAAACTCGGCGTAACCGCCGCTTCGTTCGCTCCCGCCGCCGCCGTTGCTGCTCCCGCCGCCGCGGAAGCTGCTGTCGTGGAAGAACAAACCGAATTCGATGTGGTTATTGAAGAAGTTCCGAGTACCGCGAGAATTGCGGCGATTAAAGCGGTTAGGGCTTTGACGAATCTGGGATTGAAGGAAGCGAAGGAGTTGATTGAAGGTTTGCCTAAGAAATTCAAAGAAGGCGTTTCGAAAGATGAAGCTGAAGATGCTAAGAAACAACTTGAAGGTGCAGGTGCTAAAGTTAACATTGTTTAGGGTTTCGTTTTTTTTCTTCCCCAATTTTAATTTTGAAATGATGTTGTAATTTCAAGTTGTTATTATGGTGTTGTAGTGAAGAAAATTGAGTTAGTTCTGGTTTTGTTATTATTAATCTAAATTAAAAACGAATTATTTGGATGGTGTATTAATTGTATTCAAAGTGTTTTTGTTCTGTTTTATTATTTGTGCATTAATATTATCCTTTTATATTAGGATTGTTTGGATGCAAGAAATTTTGGTGTGAAAGAAAATGGATAAAAAAAATGTATTGAAATGATAAAGTAGTTAGTATTATTT
TARGET=415,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4427
PRIMER_LEFT_PENALTY=0.095574
PRIMER_RIGHT_PENALTY=0.347129
PRIMER_LEFT_SEQUENCE=ATTTCCCATCCTCAACAACAAC
PRIMER_RIGHT_SEQUENCE=CGGTACTCGGAACTTCTTCAAT
PRIMER_LEFT=231,22
PRIMER_RIGHT=509,22
PRIMER_LEFT_TM=60.096
PRIMER_RIGHT_TM=59.653
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=279
PRIMER_PAIR_PENALTY_1=0.5093
PRIMER_LEFT_1_PENALTY=0.162217
PRIMER_RIGHT_1_PENALTY=0.347129
PRIMER_LEFT_1_SEQUENCE=CGAACAAGCCAAAAACCTAGTC
PRIMER_RIGHT_1_SEQUENCE=CGGTACTCGGAACTTCTTCAAT
PRIMER_LEFT_1=346,22
PRIMER_RIGHT_1=509,22
PRIMER_LEFT_1_TM=60.162
PRIMER_RIGHT_1_TM=59.653
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=164
PRIMER_PAIR_PENALTY_2=0.5093
PRIMER_LEFT_2_PENALTY=0.162217
PRIMER_RIGHT_2_PENALTY=0.347129
PRIMER_LEFT_2_SEQUENCE=GAACAAGCCAAAAACCTAGTCG
PRIMER_RIGHT_2_SEQUENCE=CGGTACTCGGAACTTCTTCAAT
PRIMER_LEFT_2=347,22
PRIMER_RIGHT_2=509,22
PRIMER_LEFT_2_TM=60.162
PRIMER_RIGHT_2_TM=59.653
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=0.5093
PRIMER_LEFT_3_PENALTY=0.162217
PRIMER_RIGHT_3_PENALTY=0.347129
PRIMER_LEFT_3_SEQUENCE=TCGAACAAGCCAAAAACCTAGT
PRIMER_RIGHT_3_SEQUENCE=CGGTACTCGGAACTTCTTCAAT
PRIMER_LEFT_3=345,22
PRIMER_RIGHT_3=509,22
PRIMER_LEFT_3_TM=60.162
PRIMER_RIGHT_3_TM=59.653
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=165
PRIMER_PAIR_PENALTY_4=0.5093
PRIMER_LEFT_4_PENALTY=0.162217
PRIMER_RIGHT_4_PENALTY=0.347129
PRIMER_LEFT_4_SEQUENCE=ACTCGAACAAGCCAAAAACCTA
PRIMER_RIGHT_4_SEQUENCE=CGGTACTCGGAACTTCTTCAAT
PRIMER_LEFT_4=343,22
PRIMER_RIGHT_4=509,22
PRIMER_LEFT_4_TM=60.162
PRIMER_RIGHT_4_TM=59.653
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_06225 Ps_contig_mira-and-tgicl-ass_franssen_42483
SEQUENCE=AAAGTTATAAATATGTACAATAACTTTTATTTGTATTTAACCATAACAATAACAAGCCAACTTTCAATAATGCAGAGAAAAATTGACATCAACATGGACGAATATATACAAAGCACCAACCACTTTTGGATGGATGGGGACAAGTAGTAGGTCTAGAGTAGACCCATTTTAACTATAATACTACTAATAATAATGCACATAAAGATTACTATCACTACCTAACATAAACTTTATCAAATGAAAGGATTAATATGATTAGAGAAGTCACTATTACTAACTCCTGCTCCTTCATAACCCATCCATTCTTCCCAATATCCCCAATCTGGATCATTCTTTCCTCTAAATGGTTCATCATCATAACACCTCTTCTTCTTCTTCTCCTTCTCATATGTTTCTCTCTTCTTTGCTTCTTCTCTCAAATTTGTAATCGCAACAACATAAGCCTCATTGATTACGTGAAACCGCACATCACAATCTTTTCCTCTGCAAACATCTGGATGATACTGCAAAGCAAGCTGTCTAAAAGCCTTTCTAACATCAGATTCAGAAGCATCTGGTTGAATCTTTAATATCTTATAAGGATCTGTCACAGAAGAAGAAGAATAAGATGAGCAACAAACTCTAACTCTGTTCATCTTATTTTGTTTCGTTTCCTTTCTTCCAAATTGCATCCATGAAACACCAGCTCCGTTACCACCAATAACAATAGTAGCAGCAGCCATTCTTAAAATCACAAAAACCTCAAAAACCCAGAAAAGCTTTCCTAAAAAAGGATCAAACTTTGAAATAATCAACACAAATACAAAAAGACCCAGAAGAGTTTTTGTTATAAAGGACCAAACTTTAAGATTTCCAACACAAATTAACTATGACCCAGAAATGGTTTTGTTGTAAAGAACCAAACTTTGATGTGGTTTTATCAATGAAGGAAGATATTGTTTCAGGATTGGAACAATGGGCACGGCAAGTTGATTTTGATATTGACGGGGAATCTGTCCAAACTGTAATAAATAATGGGTTTAGATTTTCTTTTTTTGAGGTTTGAAGAAGAAGGAGAAAATAGAATAGATGAAAGGATATCCTCCCCGTCCGTACTCGTTGCGTTCGACTACCACCTTCGTCGTTACGTACGGAACGGAACCGAACCGGACGGAACGAGGGAGAGGACCGAACGGACGACGGAACGAGGGGAGAG
TARGET=365,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0659
PRIMER_LEFT_PENALTY=0.019797
PRIMER_RIGHT_PENALTY=0.046061
PRIMER_LEFT_SEQUENCE=TTCATAACCCATCCATTCTTCC
PRIMER_RIGHT_SEQUENCE=CAGCTTGCTTTGCAGTATCATC
PRIMER_LEFT=287,22
PRIMER_RIGHT=517,22
PRIMER_LEFT_TM=60.020
PRIMER_RIGHT_TM=60.046
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=231
PRIMER_PAIR_PENALTY_1=0.1153
PRIMER_LEFT_1_PENALTY=0.019797
PRIMER_RIGHT_1_PENALTY=0.095538
PRIMER_LEFT_1_SEQUENCE=TTCATAACCCATCCATTCTTCC
PRIMER_RIGHT_1_SEQUENCE=CTTGCTTTGCAGTATCATCCAG
PRIMER_LEFT_1=287,22
PRIMER_RIGHT_1=514,22
PRIMER_LEFT_1_TM=60.020
PRIMER_RIGHT_1_TM=59.904
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=0.1579
PRIMER_LEFT_2_PENALTY=0.019797
PRIMER_RIGHT_2_PENALTY=0.138114
PRIMER_LEFT_2_SEQUENCE=TTCATAACCCATCCATTCTTCC
PRIMER_RIGHT_2_SEQUENCE=TCAATGAGGCTTATGTTGTTGC
PRIMER_LEFT_2=287,22
PRIMER_RIGHT_2=450,22
PRIMER_LEFT_2_TM=60.020
PRIMER_RIGHT_2_TM=60.138
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.1828
PRIMER_LEFT_3_PENALTY=0.019797
PRIMER_RIGHT_3_PENALTY=0.162964
PRIMER_LEFT_3_SEQUENCE=TTCATAACCCATCCATTCTTCC
PRIMER_RIGHT_3_SEQUENCE=TTTTAGACAGCTTGCTTTGCAG
PRIMER_LEFT_3=287,22
PRIMER_RIGHT_3=524,22
PRIMER_LEFT_3_TM=60.020
PRIMER_RIGHT_3_TM=59.837
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.2667
PRIMER_LEFT_4_PENALTY=0.220657
PRIMER_RIGHT_4_PENALTY=0.046061
PRIMER_LEFT_4_SEQUENCE=ATTCTTCCCAATATCCCCAATC
PRIMER_RIGHT_4_SEQUENCE=CAGCTTGCTTTGCAGTATCATC
PRIMER_LEFT_4=301,22
PRIMER_RIGHT_4=517,22
PRIMER_LEFT_4_TM=60.221
PRIMER_RIGHT_4_TM=60.046
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_06226 Ps_contig_mira-and-tgicl-ass_franssen_42483
SEQUENCE=AAAGTTATAAATATGTACAATAACTTTTATTTGTATTTAACCATAACAATAACAAGCCAACTTTCAATAATGCAGAGAAAAATTGACATCAACATGGACGAATATATACAAAGCACCAACCACTTTTGGATGGATGGGGACAAGTAGTAGGTCTAGAGTAGACCCATTTTAACTATAATACTACTAATAATAATGCACATAAAGATTACTATCACTACCTAACATAAACTTTATCAAATGAAAGGATTAATATGATTAGAGAAGTCACTATTACTAACTCCTGCTCCTTCATAACCCATCCATTCTTCCCAATATCCCCAATCTGGATCATTCTTTCCTCTAAATGGTTCATCATCATAACACCTCTTCTTCTTCTTCTCCTTCTCATATGTTTCTCTCTTCTTTGCTTCTTCTCTCAAATTTGTAATCGCAACAACATAAGCCTCATTGATTACGTGAAACCGCACATCACAATCTTTTCCTCTGCAAACATCTGGATGATACTGCAAAGCAAGCTGTCTAAAAGCCTTTCTAACATCAGATTCAGAAGCATCTGGTTGAATCTTTAATATCTTATAAGGATCTGTCACAGAAGAAGAAGAATAAGATGAGCAACAAACTCTAACTCTGTTCATCTTATTTTGTTTCGTTTCCTTTCTTCCAAATTGCATCCATGAAACACCAGCTCCGTTACCACCAATAACAATAGTAGCAGCAGCCATTCTTAAAATCACAAAAACCTCAAAAACCCAGAAAAGCTTTCCTAAAAAAGGATCAAACTTTGAAATAATCAACACAAATACAAAAAGACCCAGAAGAGTTTTTGTTATAAAGGACCAAACTTTAAGATTTCCAACACAAATTAACTATGACCCAGAAATGGTTTTGTTGTAAAGAACCAAACTTTGATGTGGTTTTATCAATGAAGGAAGATATTGTTTCAGGATTGGAACAATGGGCACGGCAAGTTGATTTTGATATTGACGGGGAATCTGTCCAAACTGTAATAAATAATGGGTTTAGATTTTCTTTTTTTGAGGTTTGAAGAAGAAGGAGAAAATAGAATAGATGAAAGGATATCCTCCCCGTCCGTACTCGTTGCGTTCGACTACCACCTTCGTCGTTACGTACGGAACGGAACCGAACCGGACGGAACGAGGGAGAGGACCGAACGGACGACGGAACGAGGGGAGAG
TARGET=591,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2054
PRIMER_LEFT_PENALTY=0.095538
PRIMER_RIGHT_PENALTY=0.109882
PRIMER_LEFT_SEQUENCE=CTGGATGATACTGCAAAGCAAG
PRIMER_RIGHT_SEQUENCE=GATTTTAAGAATGGCTGCTGCT
PRIMER_LEFT=493,22
PRIMER_RIGHT=731,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=59.890
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=239
PRIMER_PAIR_PENALTY_1=0.2728
PRIMER_LEFT_1_PENALTY=0.162964
PRIMER_RIGHT_1_PENALTY=0.109882
PRIMER_LEFT_1_SEQUENCE=TGCAAAGCAAGCTGTCTAAAAG
PRIMER_RIGHT_1_SEQUENCE=GATTTTAAGAATGGCTGCTGCT
PRIMER_LEFT_1=504,22
PRIMER_RIGHT_1=731,22
PRIMER_LEFT_1_TM=59.837
PRIMER_RIGHT_1_TM=59.890
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=228
PRIMER_PAIR_PENALTY_2=0.3740
PRIMER_LEFT_2_PENALTY=0.264143
PRIMER_RIGHT_2_PENALTY=0.109882
PRIMER_LEFT_2_SEQUENCE=CATCTGGATGATACTGCAAAGC
PRIMER_RIGHT_2_SEQUENCE=GATTTTAAGAATGGCTGCTGCT
PRIMER_LEFT_2=490,22
PRIMER_RIGHT_2=731,22
PRIMER_LEFT_2_TM=59.736
PRIMER_RIGHT_2_TM=59.890
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.5413
PRIMER_LEFT_3_PENALTY=0.046061
PRIMER_RIGHT_3_PENALTY=0.495211
PRIMER_LEFT_3_SEQUENCE=GATGATACTGCAAAGCAAGCTG
PRIMER_RIGHT_3_SEQUENCE=AGCTTTTCTGGGTTTTTGAGGT
PRIMER_LEFT_3=496,22
PRIMER_RIGHT_3=759,22
PRIMER_LEFT_3_TM=60.046
PRIMER_RIGHT_3_TM=60.495
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=264
PRIMER_PAIR_PENALTY_4=0.5907
PRIMER_LEFT_4_PENALTY=0.095538
PRIMER_RIGHT_4_PENALTY=0.495211
PRIMER_LEFT_4_SEQUENCE=CTGGATGATACTGCAAAGCAAG
PRIMER_RIGHT_4_SEQUENCE=AGCTTTTCTGGGTTTTTGAGGT
PRIMER_LEFT_4=493,22
PRIMER_RIGHT_4=759,22
PRIMER_LEFT_4_TM=59.904
PRIMER_RIGHT_4_TM=60.495
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=267
=
PRIMER_SEQUENCE_ID=SSR_06227 Ps_contig_mira-and-tgicl-ass_franssen_42484
SEQUENCE=GTCGGGGATTCCATCTCAGTGTTCCAGTTTTCTTCTTCTTCTTTGAAATCATTAAAAACTTCATAATGAGAGAAATCCTTCATGTACAAGCTGGTCAATGTGGAAATCAAATTGGTGGAAAGTTTTGGGAGGTTATGTGTGATGAACATGGGATAGATCCTTCTGGAAGTTATGTAGGAAACTCACCCCTTCAACTTGAGAGGGTGAATGTTTACTACAATGAAGCAAGTGGTGGAAGATATGTTCCTAGAGCTGTTCTTATGGATCTTGAACCAGGTACTATGGATAGTTTGCGCTGCGGTCCGTTCGGAAAAATATTTAGGCCCGATAACTTCGTGTTCGGACAAAATGGAGCTGGTAATAATTGGGCTAAAGGACATTATACCGAAGGAGCCGAGCTTATTGATTCTGTCCTCGATGTTGTTCGTAAAGAAGCCGAGAATTGTGATTGCTTGCAAGGTTTTCAAATTTGTCATTCATTGGGAGGTGGAACTGGATCAGGAATGGGTACTTTGCTCATTTCAAAGATCAGAGAAGAGTATCCTGATAGAATGATGTTGACTTTCTCAGTGTTTCCTTCTCCGAAGGTCTCTGATACAGTCGTGGAACCCTACAATGCAACTCTCTCAGTTCATCAACTAGTTGAGAATGCTGATGAATGCATGGTTCTTGATAATGAAGCACTCTATGATATCTGTTTCAGAACTCTCAAGCTCACTAATCCAAGTTTTGGTGATTTGAACCATTTGATCTCAACAACAATGAGTGGAGTAACGTGTTGCCTTCGATTTCCCGGCCAACTCAACTCGGATCTTCGAAAACTAGCGGTTAATCTCATTCCTTTCCCACGTCTACACTTTTTCATGGTTGGTTTTGCGCCCTTAACATCAAGGGGTTCTCAACAATACAGTTCCCTCACAATTCCAGAACTGACACAACAAATGTGGGATGCAAGAAACATGATGTGTGCTGCAGATCCAAGACATGGTAGATACTTAACAGCCTCGGCAATGTTCCGCGGCAAAATGAGCACGAAAGAAGTTGATCAACAGATGATAAATGTTCAGAACAAGAACTCGTCTTACTTCGTCGAATGGATACCGAACAATGTGAAATCAAGTGTCTGTGACATTCCGCCAACGGGACTGTCGATGTCTTCGACGTTTATGGGGAATTCGACGTCGATACAAGAGATGTTTAGGCGTGTTTCGGAACAGTTTACTGTTATGTTTAAGAGAAAGGCTTTCTTGCATTGGTATACTGCTGAAGGTATGGATGAAATGGAGTTTACTGAGGCTGAGAGTAATATGAATGATTTGGTTGCTGAGTATCAACAATATCAAGATGCTGTTGCAGTGGAAGATGGTGAGGTTGATGAAGAGGAAGATGAGGAGGACATTGCTTAGAAGCAATGATTTGAGCAAAAGATTCATTAAGGTTTCTTTTGGGACACTCCTCATGTCATGGCAAATACACATATAGTAAGAAGAACTTTGTGTTTGTGAGTTACACATATTATGGGTTTGAAAGGTACATATGACTCTCAATTCTAATGGGAGTAATGTTTTTAAGGCTTTTGTTTTGAATTGTTTGGTTCTTGTTCTTTGTGGGGGTTACTTTAGAGTTTAGGTGTGGGATTTTGTTGTTTGTGAATGTACACCATGTATTTTTGTTTTGTTTTCACTTCTCATTTCTTGTTTTATATCTTATCATTGTTAATAAACGTTAATAATTGATATGATCGATTGTATAGTCGGTGGAATAAATATTTGTTGAAATTATTAACCTCATTGAAATTATTAACCTCACATGATATNC
TARGET=30,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.8870
PRIMER_LEFT_PENALTY=0.882618
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=GGATTCCATCTCAGTGTTCCAG
PRIMER_RIGHT_SEQUENCE=ATCTTCCACCACTTGCTTCATT
PRIMER_LEFT=5,22
PRIMER_RIGHT=239,22
PRIMER_LEFT_TM=60.883
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=0.9050
PRIMER_LEFT_1_PENALTY=0.882618
PRIMER_RIGHT_1_PENALTY=0.022341
PRIMER_LEFT_1_SEQUENCE=GGATTCCATCTCAGTGTTCCAG
PRIMER_RIGHT_1_SEQUENCE=CCCATGTTCATCACACATAACC
PRIMER_LEFT_1=5,22
PRIMER_RIGHT_1=151,22
PRIMER_LEFT_1_TM=60.883
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=1.0186
PRIMER_LEFT_2_PENALTY=0.882618
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=GGATTCCATCTCAGTGTTCCAG
PRIMER_RIGHT_2_SEQUENCE=TGAAGGGGTGAGTTTCCTACAT
PRIMER_LEFT_2=5,22
PRIMER_RIGHT_2=192,22
PRIMER_LEFT_2_TM=60.883
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=188
PRIMER_PAIR_PENALTY_3=1.0284
PRIMER_LEFT_3_PENALTY=0.882618
PRIMER_RIGHT_3_PENALTY=0.145769
PRIMER_LEFT_3_SEQUENCE=GGATTCCATCTCAGTGTTCCAG
PRIMER_RIGHT_3_SEQUENCE=TCTCAAGTTGAAGGGGTGAGTT
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=200,22
PRIMER_LEFT_3_TM=60.883
PRIMER_RIGHT_3_TM=60.146
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=196
PRIMER_PAIR_PENALTY_4=1.0859
PRIMER_LEFT_4_PENALTY=1.081589
PRIMER_RIGHT_4_PENALTY=0.004359
PRIMER_LEFT_4_SEQUENCE=GGATTCCATCTCAGTGTTCCA
PRIMER_RIGHT_4_SEQUENCE=ATCTTCCACCACTTGCTTCATT
PRIMER_LEFT_4=5,21
PRIMER_RIGHT_4=239,22
PRIMER_LEFT_4_TM=59.918
PRIMER_RIGHT_4_TM=60.004
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=235
=
PRIMER_SEQUENCE_ID=SSR_06228 Ps_contig_mira-and-tgicl-ass_franssen_42485
SEQUENCE=CAAAATAAAATCTAGCATATCCAAATTTCATAGAATTCAATGATGGCAATCATATTTTGAATCAAATATTGATGTTTAATTCTAACAACAAAGTGATACTTGAAGGAATTGTTTAAGCTGTCTTACTCAACTACCAATTGGTCACACATAAAAGCAAATAAACCAAAACCAATTTTACAGCCATAGTAAGTGAAACATAAGAAAACTAGTCTTAGTTTGAAAAAGCAACATGAAGTTGCAGCAGATTATAATATTCAAACTTAAATTCAAAATCAAATGTCATGTTGACCATACTCTTCCTCATCGCCTTCCTCCTCTCCATACTCATCTTCATCAGCAGTCGCATCTTGATACTGTTGATACTCTGCAACCAAATCATTCATATTGCTCTCAGCCTCAGTGAATTCCATCTCGTCCATTCCTTCACCTGTGTACCAATGCAAGAAAGCCTTCCTCCTGAACATAGCAGTAAACTGTTCACTTACCCTGCGGAACATTTCTTGAATTGAGGTCGAGTTTCCAATAAATGTTGAAGCCATCTTCAAACCAGTTGGGGGAATATCACACACAGTGGATTTGACATTGTTTGGAATCCATTCCACAAAGTATGACGAGTTCTTGTTCTGAACATTCATCATTTGTTCATCAACCTCCTTGGTACTCATCTTGCCTCTAAACATAGCAGATGCGGTGAGGTAACGTCCATGCCTAGGATCAGCAGCACACATCATGTTCTTGGCATCCCACATTTGTTGAGTGAGCTCAGGGACACTGAGAGTCCTGTACTGCTGGGAGCCGCGAGAGGTCAGAGGTGCAAACCCCACCATGAAAAAGTGAAGACGAGGGAATGGAATGAGATTCACAGCAAGCTTCCTGAGATCAGAGTTCAACTGACCAGGGAACCTCAAACAACATGTTACACCAGACATAGTTGCTGATATCAAATGATTCAGATCACCAAAGCTTGGGTTACTAAGCTTCAGAATACGGAAGCAAATGTCATACAAGGCTTCATTGTCAAGAACCATAACCTCATCTGCATTTTCAACAAGCTGGTGAACAGATAGAGTAGCATTGTAGGGTTCCACCACAGTGTCAGAAACTTTAGGAGAAGGGAAAACAGAGAAAGTAAGCATCATCCTGTCAGGGTATTCCTCCCTAATCTTTGAAATCAGAAGGGTTCCCATTCCAGATCCAGTTCCTCCACCAAGTGAATGACACACTTGAAAACCCTGCAAGCAATCACAGTTTTCAGCTTCCTTACGAACAACGTCAAGAACAGAATCAATGAGTTCCGCGCCTTCAGTATAGTGACCTTTAGCCCAGTTATTTCCAGCACCACTCTGACCAAACACAAAGTTATCCGGTCTAAAGATCTGACCGTACGGTCCAGACCGGATACTGTCCATGGTTCCCGGTTCAAGATCCATGAGAACCGCTCTGGGAACGAAGCGGCCACCACTTGCTTCGTTGTAGTAAACATCGATCCTCTCTAGTTGAAGATCTGAGTCGCCGGTGTAGCGTCCGGTTGGATCGATTCCGTGTTCGGCGCAAACGACTTCCCAGAATTTGGCACCGATTTGGTTGCCGCATTGACCACCCTGGATGTGGAGGATTTGACGCATGTTGAAGGTTTGAGAGAAGAAATTGAAGAAATGACCTAGAAACGAAAATGGGGAGAAAAGGTTCCTAGGGTGCGAAGAATCGAGAGAGATTGGGGAAAATGATTCGGTTTTATAGAGAGAGAGTAAAAAACGTTTGGAAATTATTTTATTTTTGTTTCAA
TARGET=1739,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06229 Ps_contig_mira-and-tgicl-ass_franssen_42486
SEQUENCE=TCGGGGATCCTTTGATCAATTCTATTATCTTCTTCAATCCTCTTTAAAAAAAGGAAAATCTAAACCCATTATTTACTGCAGTTTGGACAGATTCCCCATCAAAATCAAAATCAACTTGCCGTGCCCATTGTTCCAATCCTGAAAACAATATCTTCATTCATTAATAAAACCACATCAAAGTTTGTTTCTTTACAACAAAACCATTTCTGGGTCATTCTTTGTTTGTGTTGGAAATCTTAAAGTTTGGTCCTTTATAACAAAAACTCTTCTGGGTCTTTTTGTATTTGTGTTGATTATTTCAAAGTTTGATCCTTTTTTAGGAAAGCTTTTCTGGGTTGTTGAGGTTTTTGTGATTTTAAGGATGGCCGCTACTACTACTGCTGGTGTTATTGGTGGTATAGGATCTGGTGTTTCATGGATGCAATTTGGAAGAAAGGAAAAGAAACAAAATAAGATGAACAGAGTTAGAGTTTGTTGCTCATCTTATTCTTCTTCTGTGACAGATCCTTATAAGATATTAAAGGTTCAACCAGATGCTTCTGAATCTGATGTTAGAAAGGCTTTTAGACAACTTGCTTTGCAGTATCATCCAGATGTTTGCAGAGGAAAAGATTGTGATGTGCGGTTTCACGTAATCAATGAGGCTTATGTTGTTGCGATTACAAATTTGAGAGAAGAAACAAAGAAGAGAGAAACATATGAGAAGGAGAAGAAGAAGAAGAGGTGTTATGATGATGAACCATTTAGAGGAAAGAATGATCCAGATTGGGGATATTGGGAAGAATGGATGGGTTATGAAGGAGCAGGAATTAGTAATAGTGACTTCTCTAATCATATTAATCCTTTCATTTGATCAACTTTATGTTAATGTAAATTAGTATTAATTAACCCTTTTAATTATATGTTTATGTGCATTATTAGTTAAAATGGGTTCTAGACCTACTACTTGTCCCCATCCTAAAGTGGTTGGTTGGTGCTTTGTATATAGTCCTTGTTGATGTCAAAGTTTCTCTGCATAATTGAAAGTTGAGAATATTTGGCTTGTTATGANNNTATANGTTTATCTTTTTTGTTTTGTTTTCTCTYCATTTTATCCAANCNAAATTGAATAATGATAAAGAATAAAAGG
TARGET=708,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06230 Ps_contig_mira-and-tgicl-ass_franssen_42486
SEQUENCE=TCGGGGATCCTTTGATCAATTCTATTATCTTCTTCAATCCTCTTTAAAAAAAGGAAAATCTAAACCCATTATTTACTGCAGTTTGGACAGATTCCCCATCAAAATCAAAATCAACTTGCCGTGCCCATTGTTCCAATCCTGAAAACAATATCTTCATTCATTAATAAAACCACATCAAAGTTTGTTTCTTTACAACAAAACCATTTCTGGGTCATTCTTTGTTTGTGTTGGAAATCTTAAAGTTTGGTCCTTTATAACAAAAACTCTTCTGGGTCTTTTTGTATTTGTGTTGATTATTTCAAAGTTTGATCCTTTTTTAGGAAAGCTTTTCTGGGTTGTTGAGGTTTTTGTGATTTTAAGGATGGCCGCTACTACTACTGCTGGTGTTATTGGTGGTATAGGATCTGGTGTTTCATGGATGCAATTTGGAAGAAAGGAAAAGAAACAAAATAAGATGAACAGAGTTAGAGTTTGTTGCTCATCTTATTCTTCTTCTGTGACAGATCCTTATAAGATATTAAAGGTTCAACCAGATGCTTCTGAATCTGATGTTAGAAAGGCTTTTAGACAACTTGCTTTGCAGTATCATCCAGATGTTTGCAGAGGAAAAGATTGTGATGTGCGGTTTCACGTAATCAATGAGGCTTATGTTGTTGCGATTACAAATTTGAGAGAAGAAACAAAGAAGAGAGAAACATATGAGAAGGAGAAGAAGAAGAAGAGGTGTTATGATGATGAACCATTTAGAGGAAAGAATGATCCAGATTGGGGATATTGGGAAGAATGGATGGGTTATGAAGGAGCAGGAATTAGTAATAGTGACTTCTCTAATCATATTAATCCTTTCATTTGATCAACTTTATGTTAATGTAAATTAGTATTAATTAACCCTTTTAATTATATGTTTATGTGCATTATTAGTTAAAATGGGTTCTAGACCTACTACTTGTCCCCATCCTAAAGTGGTTGGTTGGTGCTTTGTATATAGTCCTTGTTGATGTCAAAGTTTCTCTGCATAATTGAAAGTTGAGAATATTTGGCTTGTTATGANNNTATANGTTTATCTTTTTTGTTTTGTTTTCTCTYCATTTTATCCAANCNAAATTGAATAATGATAAAGAATAAAAGG
TARGET=964,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06231 Ps_contig_mira-and-tgicl-ass_franssen_42487
SEQUENCE=CGGAAAACAAAAATAAAGAATGAATAGAGTTAGAGCTTGTTGCTCATCTTATTCTTCTTCTGTGACAGATCCTTATTAAGTATTATTTAAAGATTCAACCAGATGCTTCTGAATCTGATGTTAGAAAGGCTTTTAGACAGCTTGCTTTGCAGTATCATCCAGATGTTTGCAGAGGAAAAGAATTGTGATGTGCGGTTTCACGTAATCAATGAGGCTTATGTTGTGAGTTATTATTATCTATAAATTTTGATTGCAAATAGTGAATTGAATTAGTAGTAATAATATGAATGAAATGATGTTCAGGTTGCGATTACAAATTTGAGAGAAGAAGCAAAGAAGAGAGAAACATATGAGAAGGAGAAGAAGAAGAAGAGGTGTTATGATGATGAACCATTTAGAGGAAAGAATGATCCAGATTGGGGATATTGGGAAGAATGGATGGGTTATGAAGGAGCAGGAGTTAGTAATAGTGACTTCTCTAATCATATTAATCCTTTCATTTGATAAAGTTTATGTTAGGTAGTGATAGTAATCTTTATGTGCATTATTATTAGTAGTATTATAGTTAAAATGGGTCTACTCTAGACCTACTACTTGTCCCCATCCATCCAAAAGTGGTTGGTGCTTTGTATATAGTCCCTGTTGATGTCAATTTTTCTCTGAATTATTGAAAGTTGGCTTGTAATTGTTATGGTTAAATACAAATAAAAGTTATTGTAAGTANTTATTCTTCTAAA
TARGET=359,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2667
PRIMER_LEFT_PENALTY=0.046061
PRIMER_RIGHT_PENALTY=0.220657
PRIMER_LEFT_SEQUENCE=CAGCTTGCTTTGCAGTATCATC
PRIMER_RIGHT_SEQUENCE=ATTCTTCCCAATATCCCCAATC
PRIMER_LEFT=137,22
PRIMER_RIGHT=435,22
PRIMER_LEFT_TM=60.046
PRIMER_RIGHT_TM=60.221
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=299
PRIMER_PAIR_PENALTY_1=0.3162
PRIMER_LEFT_1_PENALTY=0.095538
PRIMER_RIGHT_1_PENALTY=0.220657
PRIMER_LEFT_1_SEQUENCE=CTTGCTTTGCAGTATCATCCAG
PRIMER_RIGHT_1_SEQUENCE=ATTCTTCCCAATATCCCCAATC
PRIMER_LEFT_1=140,22
PRIMER_RIGHT_1=435,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=60.221
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=296
PRIMER_PAIR_PENALTY_2=0.5259
PRIMER_LEFT_2_PENALTY=0.506150
PRIMER_RIGHT_2_PENALTY=0.019797
PRIMER_LEFT_2_SEQUENCE=CAGATGTTTGCAGAGGAAAAGA
PRIMER_RIGHT_2_SEQUENCE=TTCATAACCCATCCATTCTTCC
PRIMER_LEFT_2=159,22
PRIMER_RIGHT_2=449,22
PRIMER_LEFT_2_TM=59.494
PRIMER_RIGHT_2_TM=60.020
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=291
PRIMER_PAIR_PENALTY_3=0.5356
PRIMER_LEFT_3_PENALTY=0.162964
PRIMER_RIGHT_3_PENALTY=0.372603
PRIMER_LEFT_3_SEQUENCE=TTTTAGACAGCTTGCTTTGCAG
PRIMER_RIGHT_3_SEQUENCE=TATCCCCAATCTGGATCATTCT
PRIMER_LEFT_3=130,22
PRIMER_RIGHT_3=424,22
PRIMER_LEFT_3_TM=59.837
PRIMER_RIGHT_3_TM=59.627
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=295
PRIMER_PAIR_PENALTY_4=0.6262
PRIMER_LEFT_4_PENALTY=0.046061
PRIMER_RIGHT_4_PENALTY=0.580189
PRIMER_LEFT_4_SEQUENCE=CAGCTTGCTTTGCAGTATCATC
PRIMER_RIGHT_4_SEQUENCE=CCAATCTGGATCATTCTTTCCT
PRIMER_LEFT_4=137,22
PRIMER_RIGHT_4=419,22
PRIMER_LEFT_4_TM=60.046
PRIMER_RIGHT_4_TM=59.420
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_06232 Ps_contig_mira-and-tgicl-ass_franssen_42488
SEQUENCE=GTTCCTAAAGAAGAACAAATAAAACTAAGTACGAAAGGAATCGTACAACTCATACATAGTGTTAAAATCAGGCACTTCTGTGATGTCCACAAGGTATATCACGGCAAGGTTCTTTATTGTCTCTGCAACCGATGACAGCACCTCATCCATCTGCATACAAGTCTCGTCCCAATCGTGTCCAAAACGGATGACAACGAGACGCTCTTCCTCCGCAAGGATCGCCTGATCTACAGCCCACCCCGAATGCAAGTGAGGCAACATGTACGACATCTTTCTTGTTCTTAATCACAACTCTACTTTAGTGAGTAAGGGAAGAATGAAATGAAATGATGAGATATTGTTCATGTATGTACCCCG
TARGET=316,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.6884
PRIMER_LEFT_PENALTY=0.108206
PRIMER_RIGHT_PENALTY=2.580205
PRIMER_LEFT_SEQUENCE=ATACAAGTCTCGTCCCAATCGT
PRIMER_RIGHT_SEQUENCE=CGGGGTACATACATGAACAATATC
PRIMER_LEFT=154,22
PRIMER_RIGHT=356,24
PRIMER_LEFT_TM=59.892
PRIMER_RIGHT_TM=59.420
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=41.667
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.0000
PRIMER_RIGHT_END_STABILITY=5.5000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=2.6975
PRIMER_LEFT_1_PENALTY=0.117281
PRIMER_RIGHT_1_PENALTY=2.580205
PRIMER_LEFT_1_SEQUENCE=TCTTTATTGTCTCTGCAACCGA
PRIMER_RIGHT_1_SEQUENCE=CGGGGTACATACATGAACAATATC
PRIMER_LEFT_1=110,22
PRIMER_RIGHT_1=356,24
PRIMER_LEFT_1_TM=59.883
PRIMER_RIGHT_1_TM=59.420
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=41.667
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=5.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=247
PRIMER_PAIR_PENALTY_2=2.7111
PRIMER_LEFT_2_PENALTY=0.130883
PRIMER_RIGHT_2_PENALTY=2.580205
PRIMER_LEFT_2_SEQUENCE=TTTATTGTCTCTGCAACCGATG
PRIMER_RIGHT_2_SEQUENCE=CGGGGTACATACATGAACAATATC
PRIMER_LEFT_2=112,22
PRIMER_RIGHT_2=356,24
PRIMER_LEFT_2_TM=60.131
PRIMER_RIGHT_2_TM=59.420
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=41.667
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.6000
PRIMER_RIGHT_2_END_STABILITY=5.5000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=245
PRIMER_PAIR_PENALTY_3=2.7200
PRIMER_LEFT_3_PENALTY=0.139753
PRIMER_RIGHT_3_PENALTY=2.580205
PRIMER_LEFT_3_SEQUENCE=TATTGTCTCTGCAACCGATGAC
PRIMER_RIGHT_3_SEQUENCE=CGGGGTACATACATGAACAATATC
PRIMER_LEFT_3=114,22
PRIMER_RIGHT_3=356,24
PRIMER_LEFT_3_TM=60.140
PRIMER_RIGHT_3_TM=59.420
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=41.667
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.3000
PRIMER_RIGHT_3_END_STABILITY=5.5000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=2.7660
PRIMER_LEFT_4_PENALTY=0.108206
PRIMER_RIGHT_4_PENALTY=2.657811
PRIMER_LEFT_4_SEQUENCE=ATACAAGTCTCGTCCCAATCGT
PRIMER_RIGHT_4_SEQUENCE=CGGGGTACATACATGAACAATA
PRIMER_LEFT_4=154,22
PRIMER_RIGHT_4=356,22
PRIMER_LEFT_4_TM=59.892
PRIMER_RIGHT_4_TM=57.342
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.0000
PRIMER_RIGHT_4_END_STABILITY=6.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_06233 Ps_contig_mira-and-tgicl-ass_franssen_42489
SEQUENCE=CGTAATAATATTGTAAAAATAGAACGATCAATGTGCAGCATCTTCTTCCTCACAAAACTTTAGTGTACTCTTTTTGTCACCCCAAACCAAGGAATTCTAATTCATCTGGACTAGTTGGGCTTATTTTTATCATCCATCCATCTTCATAAGGGGGCTTGAATTGATGAGGCCAGGCTTTCCGGTGAGCCCAGTATTAACCTCGATAACCTCACCGGAAATAGGAGAGTTTACACCACTTGTTGCCTTCACACTTTCAACAGCTCCGAAACCTTTACCCTTTGTAACTGAGACACCAGGTTCTGGAAGCTCCACAAACACAACCTCTCCAAGATGGTCCTGGNATGGTCATGATAACCAATAGTGGCTACTGNTCCTTCANGCTTGACCCATTNATGTGAAGGTGCTACTTGAACTA
TARGET=131,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1708
PRIMER_LEFT_PENALTY=0.130358
PRIMER_RIGHT_PENALTY=0.040447
PRIMER_LEFT_SEQUENCE=TTCATCTGGACTAGTTGGGCTT
PRIMER_RIGHT_SEQUENCE=TCTCCTATTTCCGGTGAGGTTA
PRIMER_LEFT=100,22
PRIMER_RIGHT=224,22
PRIMER_LEFT_TM=60.130
PRIMER_RIGHT_TM=59.960
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=125
PRIMER_PAIR_PENALTY_1=0.2043
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.073989
PRIMER_LEFT_1_SEQUENCE=TTCATCTGGACTAGTTGGGCTT
PRIMER_RIGHT_1_SEQUENCE=GTGAAGGCAACAAGTGGTGTAA
PRIMER_LEFT_1=100,22
PRIMER_RIGHT_1=248,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=0.2298
PRIMER_LEFT_2_PENALTY=0.130358
PRIMER_RIGHT_2_PENALTY=0.099438
PRIMER_LEFT_2_SEQUENCE=TTCATCTGGACTAGTTGGGCTT
PRIMER_RIGHT_2_SEQUENCE=ACCATCTTGGAGAGGTTGTGTT
PRIMER_LEFT_2=100,22
PRIMER_RIGHT_2=334,22
PRIMER_LEFT_2_TM=60.130
PRIMER_RIGHT_2_TM=59.901
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=235
PRIMER_PAIR_PENALTY_3=0.2578
PRIMER_LEFT_3_PENALTY=0.130358
PRIMER_RIGHT_3_PENALTY=0.127450
PRIMER_LEFT_3_SEQUENCE=TTCATCTGGACTAGTTGGGCTT
PRIMER_RIGHT_3_SEQUENCE=TACAAAGGGTAAAGGTTTCGGA
PRIMER_LEFT_3=100,22
PRIMER_RIGHT_3=282,22
PRIMER_LEFT_3_TM=60.130
PRIMER_RIGHT_3_TM=59.873
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=183
PRIMER_PAIR_PENALTY_4=0.3156
PRIMER_LEFT_4_PENALTY=0.130358
PRIMER_RIGHT_4_PENALTY=0.185267
PRIMER_LEFT_4_SEQUENCE=TTCATCTGGACTAGTTGGGCTT
PRIMER_RIGHT_4_SEQUENCE=TCTTGGAGAGGTTGTGTTTGTG
PRIMER_LEFT_4=100,22
PRIMER_RIGHT_4=330,22
PRIMER_LEFT_4_TM=60.130
PRIMER_RIGHT_4_TM=60.185
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=231
=
PRIMER_SEQUENCE_ID=SSR_06234 Ps_contig_mira-and-tgicl-ass_franssen_42490
SEQUENCE=TGATTCTTCTACACACTCCTCACAAACACAACCCTTAACAATAACAATGGCACTTAGGATGTGGGCTTCTTCAACTGCCAATGCACTCAAACTCTCCTCTTCCTCTAGATTACATCTCTCCCCTACCTTTTCCATCTCCAGATGTTTCTCTAATGTTTTGGATGGACTCAAGTACGCACCTTTYATYNTGAATCATCAAACATATATACATACATTCATTGAATCTTTAAGGTTTTGGATGGACTCAAGTACGCACCTTCACATGAATGGGTCAAGCATGAAGGATCAGTAGCCACTATTGGTATCACTGACCATGCCCAGGTTTGTTTCCTCCACCTCTATATGTATGTTGTTCTGTCTGTGCTTTATTGTTTGTTTGTTACAGGACCATCTTGGAGAGGTTGTGTTTGTGGAGCTTCCAGAACCTGGTGTCTCAGTTACAAAGGGTAAAGGTTTCGGAGCTGTTGAAAGTGTGAAGGCAACAAGTGATGTAAACTCTCCTATTTCCGGTGAGGTTATCGAGGTTAATACTGGGCTCACCGGAAAGCCTGGCCTCATCAAGTTCATTCCTTCACTCTCACCTTTANGACTAATTAACTACTTATTTCAATATGAAAAATACTCAACTGGANCTGAGTTGAAAGTGTGAAGGCAACAAGTGATGTAAACTCTCCTATTTTCCGGTGAGGTTATCGAGGTTAATACTGGGCTCACCGGAAAGCCTGGCCTCATCAATTCAAGCCCCTATGAAGATGGATGGATGATAAAAATAAAGCCAACTAGTCCAGATGAATTAGAATCCTTGTTGGGTGCAAAAGAGTACACAAAGTTTTGTGAGGAAGAAGATGCTGCACATTGATGTTCATTTTTCAATATATTGAACTGACTTGTTTTTAATATTGTACTACTATCTATCATCTTGTTGTCTAATTTTTTTACAAATGCAATAGAATTGCTTCAAGTTATTGTTGTTTCCATGCGACACTTCAGTGTGTCAGTCTATCTAGCTTGTCATGTACTAATTAAGAAGCAAATTTTGTTTTTTCTCAATCCAGTTATTATTTTCACTTCAATGTTTTCACAATGTATTTTTGRTAAACAAAGTTCAGATCGGTCAACAATAAAAAGT
TARGET=369,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06235 Ps_contig_mira-and-tgicl-ass_franssen_42490
SEQUENCE=TGATTCTTCTACACACTCCTCACAAACACAACCCTTAACAATAACAATGGCACTTAGGATGTGGGCTTCTTCAACTGCCAATGCACTCAAACTCTCCTCTTCCTCTAGATTACATCTCTCCCCTACCTTTTCCATCTCCAGATGTTTCTCTAATGTTTTGGATGGACTCAAGTACGCACCTTTYATYNTGAATCATCAAACATATATACATACATTCATTGAATCTTTAAGGTTTTGGATGGACTCAAGTACGCACCTTCACATGAATGGGTCAAGCATGAAGGATCAGTAGCCACTATTGGTATCACTGACCATGCCCAGGTTTGTTTCCTCCACCTCTATATGTATGTTGTTCTGTCTGTGCTTTATTGTTTGTTTGTTACAGGACCATCTTGGAGAGGTTGTGTTTGTGGAGCTTCCAGAACCTGGTGTCTCAGTTACAAAGGGTAAAGGTTTCGGAGCTGTTGAAAGTGTGAAGGCAACAAGTGATGTAAACTCTCCTATTTCCGGTGAGGTTATCGAGGTTAATACTGGGCTCACCGGAAAGCCTGGCCTCATCAAGTTCATTCCTTCACTCTCACCTTTANGACTAATTAACTACTTATTTCAATATGAAAAATACTCAACTGGANCTGAGTTGAAAGTGTGAAGGCAACAAGTGATGTAAACTCTCCTATTTTCCGGTGAGGTTATCGAGGTTAATACTGGGCTCACCGGAAAGCCTGGCCTCATCAATTCAAGCCCCTATGAAGATGGATGGATGATAAAAATAAAGCCAACTAGTCCAGATGAATTAGAATCCTTGTTGGGTGCAAAAGAGTACACAAAGTTTTGTGAGGAAGAAGATGCTGCACATTGATGTTCATTTTTCAATATATTGAACTGACTTGTTTTTAATATTGTACTACTATCTATCATCTTGTTGTCTAATTTTTTTACAAATGCAATAGAATTGCTTCAAGTTATTGTTGTTTCCATGCGACACTTCAGTGTGTCAGTCTATCTAGCTTGTCATGTACTAATTAAGAAGCAAATTTTGTTTTTTCTCAATCCAGTTATTATTTTCACTTCAATGTTTTCACAATGTATTTTTGRTAAACAAAGTTCAGATCGGTCAACAATAAAAAGT
TARGET=752,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06236 Ps_contig_mira-and-tgicl-ass_franssen_42491
SEQUENCE=ATAGAAAGGATGAGGCATTTGCTATCCACGATTCATCAAAGATAATATATTTGGGGAAAAGGAAAACACACACTGACATACATGAACAATATCTCATCATTTCATTTCATTCTTCTACACACTCCTCACAAACACAACCCTTAACAATAACAATGGCACTTAGGATGTGGGCTTCTTCAACTGCCAATGCACTCAAACTCTCCTCTTCCTCTAGATTACATCTCTCCCCTACCTTTTCCATCTCCAGATGTTTCTCTAATGTTTTGGATGGACTCAAGTACGCACCTTCACATGAATGGGTCAAGCATGAAGGATCAGTAGCCACTATTGGTATCACTGACCATGCCCAGGACCATCTTGGAGAGGTTGTGTTTGTGGAGCTTCCAGAACCTGGTGTCTCAGTTACAAAGGGTAAAGGTTTCGGAGCTGTTGAAAGTGTGAAGGCAACAAGTGATGTAAACTCTCCTATTTCCGGTGAGGTTATCGAGGTTAATACTGGGCTCACCGGAAAGCCTGGCCTCATCAATTCAAGCCCCTATGAAGATGGATGGATGATAAAAATAAAGCCAACTAGTCCAGATGAATTAGAATCCTTGTTGGGTGCAAAAGAGTACACAAAGTTTTGTGAGGAAGAAGATGCTGCACATTGATGTTCATTTTTCAATATATTGAACTGACTGTTTTTAATATTGTACTACTATCTATCATCTTGTTGTCTATTTTTTTTCAAATGCAATAGAATTGCTTCAARNTATTGTTGTTTCCATGCGACACTTCAGTGTGTCAGTCTATCTAGCTTGTCATGTACTAATTAAGAAGCAAATTTGTTTTTTCTCATCCAGTTATTATTTTCACTTCAATGTTTTCACAATGATTTTTGATAACAAAGTTCAGATCGGTCA
TARGET=97,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06237 Ps_contig_mira-and-tgicl-ass_franssen_42491
SEQUENCE=ATAGAAAGGATGAGGCATTTGCTATCCACGATTCATCAAAGATAATATATTTGGGGAAAAGGAAAACACACACTGACATACATGAACAATATCTCATCATTTCATTTCATTCTTCTACACACTCCTCACAAACACAACCCTTAACAATAACAATGGCACTTAGGATGTGGGCTTCTTCAACTGCCAATGCACTCAAACTCTCCTCTTCCTCTAGATTACATCTCTCCCCTACCTTTTCCATCTCCAGATGTTTCTCTAATGTTTTGGATGGACTCAAGTACGCACCTTCACATGAATGGGTCAAGCATGAAGGATCAGTAGCCACTATTGGTATCACTGACCATGCCCAGGACCATCTTGGAGAGGTTGTGTTTGTGGAGCTTCCAGAACCTGGTGTCTCAGTTACAAAGGGTAAAGGTTTCGGAGCTGTTGAAAGTGTGAAGGCAACAAGTGATGTAAACTCTCCTATTTCCGGTGAGGTTATCGAGGTTAATACTGGGCTCACCGGAAAGCCTGGCCTCATCAATTCAAGCCCCTATGAAGATGGATGGATGATAAAAATAAAGCCAACTAGTCCAGATGAATTAGAATCCTTGTTGGGTGCAAAAGAGTACACAAAGTTTTGTGAGGAAGAAGATGCTGCACATTGATGTTCATTTTTCAATATATTGAACTGACTGTTTTTAATATTGTACTACTATCTATCATCTTGTTGTCTATTTTTTTTCAAATGCAATAGAATTGCTTCAARNTATTGTTGTTTCCATGCGACACTTCAGTGTGTCAGTCTATCTAGCTTGTCATGTACTAATTAAGAAGCAAATTTGTTTTTTCTCATCCAGTTATTATTTTCACTTCAATGTTTTCACAATGATTTTTGATAACAAAGTTCAGATCGGTCA
TARGET=543,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06238 Ps_contig_mira-and-tgicl-ass_franssen_42493
SEQUENCE=AATTCTCTTTATTTTCTAGATCGATTCTCCCAATTTAATAGAAAAACACATTAAGCACAAAATAACACAATAATTTTAAGAAAATATTCATTATAGAGCAAATCTACACTAATCACATGATAAATATGAAGTATAAATATTATATTAAAATTTTCAACAAATAAAACTCATAGAAAACCCAAGTGAATGTTAGATTAAGTGTTAGAAATAGTGTTTTAGCAGTCCTTTTTTTGTTTGTTTATTTGTATCCATAAGTTTAGCTTGAATATAAAGATCAGTAGAGAAACATTTAATGAATTGATTCTACACTAATAATACTAATAACAAGATTGTTATAAAGTAAAAGAGGATGAAAGTATAGTACATAGAAAGGATGAGGCATTTGCTATCCACCATTCATCAAAGATAATATATTTGGGGAAANGGANANCACACGCAGACATACATGAACAATATCTCATCATTTCATTTCATTCTTCTACACACTCCTCACAAACACAACCCTTAACAATAACAATGGCACTTAGGATGTGGGCTTCTTCAACTGCCAATGCACTCAAACTCTCCTCTTCCTCTAGATTACATCTCTCCCCTACCTTTTCCATCTCCAGATGTTTCTCTAATGGTATTATTTCTCTTCACATTTTACCTTTTATATTGAATCATCAAACATATATACATACATTCACTGAATTTGCAGTATTGGATGGACTCAAGTACGCACCTTCACATGAATGGGTCAAGCATGAAGGATCAGTAGCCACTATTGGTATCACTGACCATGCCCAGGTTTGTTTCCTCCACCTCTATATGTATGTTGTTCTGTCTGTGCTTTATTGTTTGTTTGTTACAGGACCATCTTGGAGAGGTTGTGTTTGTGGAGCTTCCAGAACCTGGTGTCTCAGTTACAAAGGGTAAAGGTTTCGGAGCTGTTGAAAGTGTGAAGGCAACAAGTGATGTAAACTCTCCTATTTCCGGTGAGGTTATCGAGGTTAATACGGGGCTCACCGGAAAGCCTGGCCTCGTAAGTTCATTCCTTCACTCTCACCTTTACGACTAATTAACTACTTATTTCAATATGAAAAATAAGCCGGTTGGATAACTCAACTGGATCTGAGTTCAAACTCTGATGATGAACATAACAAGTAATTAGTACTAGTATTTACCGTTTGAGAAAGTAACTTAATTTTTCTACTTTTGTTGCTTGAAGATCAATTCAAGCCCCTATGAAGATGGATGGATGATAAAAATAAAGCCAACTAGTCCAGATGAATTAGAATCCTTGTTGGGTGCAAAAGAGTACACAAAGTTTTGTGAGGAAGAAGATGCTGCACATTGATGTTCATTTTTCAATATATTGAACTGACTTTTTTTAATATTGTACTACTATCTATCATCCTGTTGTCTATTTTTTTTCAAATGCAATAGAATTGCTTCAAGTTATTGTTGTTTCCATGCGACACTTCAGTGTGTCAGTCTATCTAGCTTGTCATGTACTAATTAAGAAGCAAATTTGTTTTTTCTCATCCAGTTATTATTTTCACTTCAATGTTTTCACAATGATTTTTGATAACAAAGTTCAGATCGGTCAACATGTATGCGTCTGTGTCTCTACATGGCTTATGTTACTTTAATAATTCTTGCAAGACAATTGTCCATAGGCCTTGCGACTGGCAGTTAGGTAACTGGACAGTCCGGATTAATCCTCTCCATCCTGTACCATAAAATCAAATTGAATGAATTATTTATTAATTTAATTTTCTTAGCAATATGAATATGTAGCTATAGCTTTAAAAAAAACTTAAGCTT
TARGET=461,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2567
PRIMER_LEFT_PENALTY=0.217974
PRIMER_RIGHT_PENALTY=0.038744
PRIMER_LEFT_SEQUENCE=NCACACGCAGACATACATGAAC
PRIMER_RIGHT_SEQUENCE=AGATGGAAAAGGTAGGGGAGAG
PRIMER_LEFT=429,22
PRIMER_RIGHT=606,22
PRIMER_LEFT_TM=60.218
PRIMER_RIGHT_TM=59.961
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.2628
PRIMER_LEFT_1_PENALTY=0.224014
PRIMER_RIGHT_1_PENALTY=0.038744
PRIMER_LEFT_1_SEQUENCE=AGAAAGGATGAGGCATTTGCTA
PRIMER_RIGHT_1_SEQUENCE=AGATGGAAAAGGTAGGGGAGAG
PRIMER_LEFT_1=366,22
PRIMER_RIGHT_1=606,22
PRIMER_LEFT_1_TM=60.224
PRIMER_RIGHT_1_TM=59.961
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=241
PRIMER_PAIR_PENALTY_2=0.2628
PRIMER_LEFT_2_PENALTY=0.224014
PRIMER_RIGHT_2_PENALTY=0.038744
PRIMER_LEFT_2_SEQUENCE=TAGAAAGGATGAGGCATTTGCT
PRIMER_RIGHT_2_SEQUENCE=AGATGGAAAAGGTAGGGGAGAG
PRIMER_LEFT_2=365,22
PRIMER_RIGHT_2=606,22
PRIMER_LEFT_2_TM=60.224
PRIMER_RIGHT_2_TM=59.961
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=242
PRIMER_PAIR_PENALTY_3=0.2860
PRIMER_LEFT_3_PENALTY=0.224014
PRIMER_RIGHT_3_PENALTY=0.061961
PRIMER_LEFT_3_SEQUENCE=AGAAAGGATGAGGCATTTGCTA
PRIMER_RIGHT_3_SEQUENCE=AAGGGTTGTGTTTGTGAGGAGT
PRIMER_LEFT_3=366,22
PRIMER_RIGHT_3=505,22
PRIMER_LEFT_3_TM=60.224
PRIMER_RIGHT_3_TM=59.938
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.5000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=140
PRIMER_PAIR_PENALTY_4=0.2860
PRIMER_LEFT_4_PENALTY=0.224014
PRIMER_RIGHT_4_PENALTY=0.061961
PRIMER_LEFT_4_SEQUENCE=TAGAAAGGATGAGGCATTTGCT
PRIMER_RIGHT_4_SEQUENCE=AAGGGTTGTGTTTGTGAGGAGT
PRIMER_LEFT_4=365,22
PRIMER_RIGHT_4=505,22
PRIMER_LEFT_4_TM=60.224
PRIMER_RIGHT_4_TM=59.938
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=141
=
PRIMER_SEQUENCE_ID=SSR_06239 Ps_contig_mira-and-tgicl-ass_franssen_42493
SEQUENCE=AATTCTCTTTATTTTCTAGATCGATTCTCCCAATTTAATAGAAAAACACATTAAGCACAAAATAACACAATAATTTTAAGAAAATATTCATTATAGAGCAAATCTACACTAATCACATGATAAATATGAAGTATAAATATTATATTAAAATTTTCAACAAATAAAACTCATAGAAAACCCAAGTGAATGTTAGATTAAGTGTTAGAAATAGTGTTTTAGCAGTCCTTTTTTTGTTTGTTTATTTGTATCCATAAGTTTAGCTTGAATATAAAGATCAGTAGAGAAACATTTAATGAATTGATTCTACACTAATAATACTAATAACAAGATTGTTATAAAGTAAAAGAGGATGAAAGTATAGTACATAGAAAGGATGAGGCATTTGCTATCCACCATTCATCAAAGATAATATATTTGGGGAAANGGANANCACACGCAGACATACATGAACAATATCTCATCATTTCATTTCATTCTTCTACACACTCCTCACAAACACAACCCTTAACAATAACAATGGCACTTAGGATGTGGGCTTCTTCAACTGCCAATGCACTCAAACTCTCCTCTTCCTCTAGATTACATCTCTCCCCTACCTTTTCCATCTCCAGATGTTTCTCTAATGGTATTATTTCTCTTCACATTTTACCTTTTATATTGAATCATCAAACATATATACATACATTCACTGAATTTGCAGTATTGGATGGACTCAAGTACGCACCTTCACATGAATGGGTCAAGCATGAAGGATCAGTAGCCACTATTGGTATCACTGACCATGCCCAGGTTTGTTTCCTCCACCTCTATATGTATGTTGTTCTGTCTGTGCTTTATTGTTTGTTTGTTACAGGACCATCTTGGAGAGGTTGTGTTTGTGGAGCTTCCAGAACCTGGTGTCTCAGTTACAAAGGGTAAAGGTTTCGGAGCTGTTGAAAGTGTGAAGGCAACAAGTGATGTAAACTCTCCTATTTCCGGTGAGGTTATCGAGGTTAATACGGGGCTCACCGGAAAGCCTGGCCTCGTAAGTTCATTCCTTCACTCTCACCTTTACGACTAATTAACTACTTATTTCAATATGAAAAATAAGCCGGTTGGATAACTCAACTGGATCTGAGTTCAAACTCTGATGATGAACATAACAAGTAATTAGTACTAGTATTTACCGTTTGAGAAAGTAACTTAATTTTTCTACTTTTGTTGCTTGAAGATCAATTCAAGCCCCTATGAAGATGGATGGATGATAAAAATAAAGCCAACTAGTCCAGATGAATTAGAATCCTTGTTGGGTGCAAAAGAGTACACAAAGTTTTGTGAGGAAGAAGATGCTGCACATTGATGTTCATTTTTCAATATATTGAACTGACTTTTTTTAATATTGTACTACTATCTATCATCCTGTTGTCTATTTTTTTTCAAATGCAATAGAATTGCTTCAAGTTATTGTTGTTTCCATGCGACACTTCAGTGTGTCAGTCTATCTAGCTTGTCATGTACTAATTAAGAAGCAAATTTGTTTTTTCTCATCCAGTTATTATTTTCACTTCAATGTTTTCACAATGATTTTTGATAACAAAGTTCAGATCGGTCAACATGTATGCGTCTGTGTCTCTACATGGCTTATGTTACTTTAATAATTCTTGCAAGACAATTGTCCATAGGCCTTGCGACTGGCAGTTAGGTAACTGGACAGTCCGGATTAATCCTCTCCATCCTGTACCATAAAATCAAATTGAATGAATTATTTATTAATTTAATTTTCTTAGCAATATGAATATGTAGCTATAGCTTTAAAAAAAACTTAAGCTT
TARGET=837,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1727
PRIMER_LEFT_PENALTY=0.132262
PRIMER_RIGHT_PENALTY=0.040447
PRIMER_LEFT_SEQUENCE=TGCAGTATTGGATGGACTCAAG
PRIMER_RIGHT_SEQUENCE=TAACCTCACCGGAAATAGGAGA
PRIMER_LEFT=695,22
PRIMER_RIGHT=985,22
PRIMER_LEFT_TM=60.132
PRIMER_RIGHT_TM=59.960
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=291
PRIMER_PAIR_PENALTY_1=0.2131
PRIMER_LEFT_1_PENALTY=0.132262
PRIMER_RIGHT_1_PENALTY=0.080808
PRIMER_LEFT_1_SEQUENCE=TGCAGTATTGGATGGACTCAAG
PRIMER_RIGHT_1_SEQUENCE=ACATCACTTGTTGCCTTCACAC
PRIMER_LEFT_1=695,22
PRIMER_RIGHT_1=959,22
PRIMER_LEFT_1_TM=60.132
PRIMER_RIGHT_1_TM=60.081
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=265
PRIMER_PAIR_PENALTY_2=0.2240
PRIMER_LEFT_2_PENALTY=0.038744
PRIMER_RIGHT_2_PENALTY=0.185267
PRIMER_LEFT_2_SEQUENCE=CTCTCCCCTACCTTTTCCATCT
PRIMER_RIGHT_2_SEQUENCE=CACAAACACAACCTCTCCAAGA
PRIMER_LEFT_2=585,22
PRIMER_RIGHT_2=879,22
PRIMER_LEFT_2_TM=59.961
PRIMER_RIGHT_2_TM=60.185
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=295
PRIMER_PAIR_PENALTY_3=0.2597
PRIMER_LEFT_3_PENALTY=0.132262
PRIMER_RIGHT_3_PENALTY=0.127450
PRIMER_LEFT_3_SEQUENCE=TGCAGTATTGGATGGACTCAAG
PRIMER_RIGHT_3_SEQUENCE=TCCGAAACCTTTACCCTTTGTA
PRIMER_LEFT_3=695,22
PRIMER_RIGHT_3=927,22
PRIMER_LEFT_3_TM=60.132
PRIMER_RIGHT_3_TM=59.873
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.3130
PRIMER_LEFT_4_PENALTY=0.132262
PRIMER_RIGHT_4_PENALTY=0.180765
PRIMER_LEFT_4_SEQUENCE=TGCAGTATTGGATGGACTCAAG
PRIMER_RIGHT_4_SEQUENCE=CACTTGTTGCCTTCACACTTTC
PRIMER_LEFT_4=695,22
PRIMER_RIGHT_4=955,22
PRIMER_LEFT_4_TM=60.132
PRIMER_RIGHT_4_TM=59.819
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=261
=
PRIMER_SEQUENCE_ID=SSR_06240 Ps_contig_mira-and-tgicl-ass_franssen_42493
SEQUENCE=AATTCTCTTTATTTTCTAGATCGATTCTCCCAATTTAATAGAAAAACACATTAAGCACAAAATAACACAATAATTTTAAGAAAATATTCATTATAGAGCAAATCTACACTAATCACATGATAAATATGAAGTATAAATATTATATTAAAATTTTCAACAAATAAAACTCATAGAAAACCCAAGTGAATGTTAGATTAAGTGTTAGAAATAGTGTTTTAGCAGTCCTTTTTTTGTTTGTTTATTTGTATCCATAAGTTTAGCTTGAATATAAAGATCAGTAGAGAAACATTTAATGAATTGATTCTACACTAATAATACTAATAACAAGATTGTTATAAAGTAAAAGAGGATGAAAGTATAGTACATAGAAAGGATGAGGCATTTGCTATCCACCATTCATCAAAGATAATATATTTGGGGAAANGGANANCACACGCAGACATACATGAACAATATCTCATCATTTCATTTCATTCTTCTACACACTCCTCACAAACACAACCCTTAACAATAACAATGGCACTTAGGATGTGGGCTTCTTCAACTGCCAATGCACTCAAACTCTCCTCTTCCTCTAGATTACATCTCTCCCCTACCTTTTCCATCTCCAGATGTTTCTCTAATGGTATTATTTCTCTTCACATTTTACCTTTTATATTGAATCATCAAACATATATACATACATTCACTGAATTTGCAGTATTGGATGGACTCAAGTACGCACCTTCACATGAATGGGTCAAGCATGAAGGATCAGTAGCCACTATTGGTATCACTGACCATGCCCAGGTTTGTTTCCTCCACCTCTATATGTATGTTGTTCTGTCTGTGCTTTATTGTTTGTTTGTTACAGGACCATCTTGGAGAGGTTGTGTTTGTGGAGCTTCCAGAACCTGGTGTCTCAGTTACAAAGGGTAAAGGTTTCGGAGCTGTTGAAAGTGTGAAGGCAACAAGTGATGTAAACTCTCCTATTTCCGGTGAGGTTATCGAGGTTAATACGGGGCTCACCGGAAAGCCTGGCCTCGTAAGTTCATTCCTTCACTCTCACCTTTACGACTAATTAACTACTTATTTCAATATGAAAAATAAGCCGGTTGGATAACTCAACTGGATCTGAGTTCAAACTCTGATGATGAACATAACAAGTAATTAGTACTAGTATTTACCGTTTGAGAAAGTAACTTAATTTTTCTACTTTTGTTGCTTGAAGATCAATTCAAGCCCCTATGAAGATGGATGGATGATAAAAATAAAGCCAACTAGTCCAGATGAATTAGAATCCTTGTTGGGTGCAAAAGAGTACACAAAGTTTTGTGAGGAAGAAGATGCTGCACATTGATGTTCATTTTTCAATATATTGAACTGACTTTTTTTAATATTGTACTACTATCTATCATCCTGTTGTCTATTTTTTTTCAAATGCAATAGAATTGCTTCAAGTTATTGTTGTTTCCATGCGACACTTCAGTGTGTCAGTCTATCTAGCTTGTCATGTACTAATTAAGAAGCAAATTTGTTTTTTCTCATCCAGTTATTATTTTCACTTCAATGTTTTCACAATGATTTTTGATAACAAAGTTCAGATCGGTCAACATGTATGCGTCTGTGTCTCTACATGGCTTATGTTACTTTAATAATTCTTGCAAGACAATTGTCCATAGGCCTTGCGACTGGCAGTTAGGTAACTGGACAGTCCGGATTAATCCTCTCCATCCTGTACCATAAAATCAAATTGAATGAATTATTTATTAATTTAATTTTCTTAGCAATATGAATATGTAGCTATAGCTTTAAAAAAAACTTAAGCTT
TARGET=1232,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2101
PRIMER_LEFT_PENALTY=0.136083
PRIMER_RIGHT_PENALTY=0.073989
PRIMER_LEFT_SEQUENCE=AATAAGCCGGTTGGATAACTCA
PRIMER_RIGHT_SEQUENCE=TGTGTACTCTTTTGCACCCAAC
PRIMER_LEFT=1084,22
PRIMER_RIGHT=1305,22
PRIMER_LEFT_TM=59.864
PRIMER_RIGHT_TM=60.074
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=222
PRIMER_PAIR_PENALTY_1=0.2101
PRIMER_LEFT_1_PENALTY=0.136083
PRIMER_RIGHT_1_PENALTY=0.073989
PRIMER_LEFT_1_SEQUENCE=AATAAGCCGGTTGGATAACTCA
PRIMER_RIGHT_1_SEQUENCE=GTGTACTCTTTTGCACCCAACA
PRIMER_LEFT_1=1084,22
PRIMER_RIGHT_1=1304,22
PRIMER_LEFT_1_TM=59.864
PRIMER_RIGHT_1_TM=60.074
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.2168
PRIMER_LEFT_2_PENALTY=0.069526
PRIMER_RIGHT_2_PENALTY=0.147302
PRIMER_LEFT_2_SEQUENCE=AGATCAATTCAAGCCCCTATGA
PRIMER_RIGHT_2_SEQUENCE=AACATCAATGTGCAGCATCTTC
PRIMER_LEFT_2=1208,22
PRIMER_RIGHT_2=1342,22
PRIMER_LEFT_2_TM=59.930
PRIMER_RIGHT_2_TM=60.147
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=135
PRIMER_PAIR_PENALTY_3=0.2834
PRIMER_LEFT_3_PENALTY=0.136083
PRIMER_RIGHT_3_PENALTY=0.147302
PRIMER_LEFT_3_SEQUENCE=AATAAGCCGGTTGGATAACTCA
PRIMER_RIGHT_3_SEQUENCE=AACATCAATGTGCAGCATCTTC
PRIMER_LEFT_3=1084,22
PRIMER_RIGHT_3=1342,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=60.147
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.3000
PRIMER_LEFT_4_PENALTY=0.069526
PRIMER_RIGHT_4_PENALTY=0.230523
PRIMER_LEFT_4_SEQUENCE=AGATCAATTCAAGCCCCTATGA
PRIMER_RIGHT_4_SEQUENCE=ACATCAATGTGCAGCATCTTCT
PRIMER_LEFT_4=1208,22
PRIMER_RIGHT_4=1341,22
PRIMER_LEFT_4_TM=59.930
PRIMER_RIGHT_4_TM=59.769
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=134
=
PRIMER_SEQUENCE_ID=SSR_06241 Ps_contig_mira-and-tgicl-ass_franssen_42494
SEQUENCE=TAGAACAATAATTGAGAATTACTTCCATCCTATAATGAAAATAACACACAAAGATGCCAAATATTTCTAGTTCTTTTACATGTCATTTCTTACAAGGCTCCAACATGAAACAACATGTTCACACTCCCAAAAGACAAGAGAATGAACTAAAACATGGTTGAGTTTTTTTGATAATAATAATGTGTTACAACAACCAAGAGCAAAGAAAAGAATATAGGTAATTAACAACATATATATATATTATATAATATAATACATTCATTCAATGAAACTTGAGACTTGTCAAGACATTGTTGTTGACAGGATCAGCCACATGATCTAACAAGTTTTGGTATGGTCCAACTCCTGTAACAAGACCTTGAATGAAGTATCCCAATATTGCCAACATAGCTAACCTTCCATTTTTCACTTCCTTAAGCTTCAACTCCTTCAAAGATTTCTCATCTTTACCGAAACCGAGAGGGTTAAAGAAAGGTCCGCCAGGGTATGCAGGGTTGCCAGAACCTCCGAAACCTTTTTCAAGTCCTAGGAAGTATTGTTTTCCCATGGAACCAGGTTTGGCCCAATCTTGGAATCTTCTGTGTTCCGCGAATCCCATCAGTGCCATTTCCAAGACGAAGAGTGTGTAATTGTCTGCCCAGTAGTTGTATGTTCCTGCAGGTGGGATCACGCCCGTTTGGAACCAAGCGAGGGCTGTTTCCTGTGGGATCAAACCGACTTTCCCGAGATATTCGGGTGCTATAGCCCCGACTGCTCCCAACATTGCGAAGCGACCGTTGATTACCTCGCCATATGCTAGCCATCTTGGCTCAATGAAACCTCCTGTTCCTTCGGGATCCGATAGTCCTAGAGGGTCAAATCCGTAGTCACCGGGTAGGCTTCCATCCAAGTAAGAAAGACTTTGCTTTGAAGCAAACCACAAGGGTCTATCAACTCCACCTTGCTTGACAGGAGGAGTAGCAGCTGCCCTGACAAGAAAGGAACCTTTTCTAGAACATCCAACTGATGAAGGAAGTGATCTTGCTCTAAAGCTCTGTCTCACAGCCTCAGCTGCAAAAGTAAGAGATGATGACGACACTAGAGCTTGTGTAGCCATTTTCTTTCCCTCTTTGTTACACCAATAGAGTGTTTTTTTTCTTCTTCAAGAATGTAGTAGATGGTTAAGAATGAATGATGGATAATGCAGTAACTAATACCCCGAAC
TARGET=230,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2867
PRIMER_LEFT_PENALTY=0.248781
PRIMER_RIGHT_PENALTY=0.037936
PRIMER_LEFT_SEQUENCE=TTCACACTCCCAAAAGACAAGA
PRIMER_RIGHT_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_LEFT=117,22
PRIMER_RIGHT=323,22
PRIMER_LEFT_TM=59.751
PRIMER_RIGHT_TM=59.962
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.2914
PRIMER_LEFT_1_PENALTY=0.253482
PRIMER_RIGHT_1_PENALTY=0.037936
PRIMER_LEFT_1_SEQUENCE=TGTCATTTCTTACAAGGCTCCA
PRIMER_RIGHT_1_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_LEFT_1=80,22
PRIMER_RIGHT_1=323,22
PRIMER_LEFT_1_TM=59.747
PRIMER_RIGHT_1_TM=59.962
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.8000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.2914
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.037936
PRIMER_LEFT_2_SEQUENCE=TCTTACAAGGCTCCAACATGAA
PRIMER_RIGHT_2_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_LEFT_2=87,22
PRIMER_RIGHT_2=323,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=59.962
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=7.8000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=237
PRIMER_PAIR_PENALTY_3=0.2914
PRIMER_LEFT_3_PENALTY=0.253482
PRIMER_RIGHT_3_PENALTY=0.037936
PRIMER_LEFT_3_SEQUENCE=TTCTTACAAGGCTCCAACATGA
PRIMER_RIGHT_3_SEQUENCE=TGTTAGATCATGTGGCTGATCC
PRIMER_LEFT_3=86,22
PRIMER_RIGHT_3=323,22
PRIMER_LEFT_3_TM=59.747
PRIMER_RIGHT_3_TM=59.962
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=238
PRIMER_PAIR_PENALTY_4=0.3446
PRIMER_LEFT_4_PENALTY=0.248781
PRIMER_RIGHT_4_PENALTY=0.095771
PRIMER_LEFT_4_SEQUENCE=TTCACACTCCCAAAAGACAAGA
PRIMER_RIGHT_4_SEQUENCE=TTAGATCATGTGGCTGATCCTG
PRIMER_LEFT_4=117,22
PRIMER_RIGHT_4=321,22
PRIMER_LEFT_4_TM=59.751
PRIMER_RIGHT_4_TM=60.096
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_06242 Ps_contig_mira-and-tgicl-ass_franssen_42497
SEQUENCE=TTTAATTTATTGATACCTNCTTAAAAATAGCACTTGAATCTTATGAAAAACAAGTACATCAATTATTCATTTTAAAAAGCACCAAATACACGGCTTAAATATCACGGTCAGAGCACATACACTTATTATGCTTAAACGAAATCATAATATTAATGCATCAGTAAATATATAGGAATGGGATATGGTTAAGAACCATATAAAAATCTCAATCTTCCTTCTTATAGTCAAAAGCAGTGTAAGGAGTGAATGCCTCTTTAATCAAACTCTTCTTCCTCCAAGTATTCCAACCCAAATCTTCGCAAATTTCATCATTGTGGTTGATACCAAATGTTGTGATGCAGTTTCTTTTATAAAACCTTGTAGAATTTTTCATTGTCTCCATGTCCTTAATTGTCTGTGAAAGCATCTTATCAGCAGTTGGGAGCTTAAATTTGTTAGCTAGTAGTCCAGATAGCCACATGGAACGTATCTCGGAGGTGTATAGATTCGAAACACTCTCAACATAACCCACAAAGGCCATGTTTGGAATCAATGGATGAACTGTTCCCCTGTACAATGACATCATACCAGAAGGGTATTCCAATAAGCTACTGAAAGGTTCTGGCAAAATGGCTTGAAGTTTCTTTTTCCCATCATAACCAGTTGCCAAAATAACTACATCAGCCTCTGTTTTAGTGTTGTCATCAAATTCAACCCCATCATTTGAAAACCACCACTTTGATGCTCTTTTGAAGATGATTTTTCCTTTGTCAGCTTCATGGAAAAAACTTTCTGGTGTTATAGCTATTTGACAAGATGCAAAATCTTCCTCAAAAGGGTGATCTGGTTTCAGTCCATACTTCTCCATTGGCTGTTTCCAAAGTAAGTAGGACTCTATGAATTTTGAAATCCCACGCCTCATTGGAGATAATAGGAGGCATAAAAGGGATTTGAGTAAACCCTGATTTGGTGTTTGATGAAGGAACTGTGCAGATCTTGTTGCATAGAACAAGAAAAATGGTACTCCCCACATCTGATAATGGGGAAGTGTCCAATGCAAACTCCTTATTATTATTGTGCATGGTTGCCCTTCAGGTCCTTGGTTTGCCTGTGCACACTCGAGAGCTAGATCAATAGCTGAATTTTTGGAACCAACGACAACAACCTTTTTTCCTTTAACAAGGTCACTGGTAGCTTCTTTGTCAAGTTTACAATAATCAATGCTATGCAGAACCTTTCCATTGAACACCTCAGGTCCCTTGTTCCGTGGAAATGTTGGCATCAATGGTATGTCACCATATTTTCCAGTGCAAATCACTACAAACTCGAAGTGGTACAGCTGAATCGTGTCTGATTCATTACTCTGAACAGAGAACTCCCACATAGGACGACCAAGAAAAGTGGATTTCCACTGTCACGATGTAGTCGTCCGAAATCAAAACCTTCTTTATCACCAATAAATTTGATTTCCACGACTTTGGAATTGAACTTAACATACTTAAACAAATCAAAATGAACTGCATAAGTATGCAAGTATTCCAAAATCTCAGCATAGGTAGGGTAATCAGAACTCTCTCTCTTAGGCCAAGGGAAATCACTGAACTCATAGTTCCATGTTTGTGATTGAAGCTTAGTACACCTATAGGAACAATGTCTCCAAACACCACCAATTGAGTCAGTGGCTTCAAAAACAACAGGGTTGTAGTTCGATAGTTGTTTCGCTACGGCTAAACCACTCACTCCAGCACCTATGATTCCAATTTTAGACACCATAACTGGTGGGTTGTCTTGGCTAGCCATAGGGCTGTGTAGTGGAAAAATAAGACTATTGTTTTATTTTGCAAAATGCGTGTGACTATGGACAGTGTCCCCGCGTACTCTGCGTTGATATTTACCTTTACTTTGTTTCTTTT
TARGET=1550,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0705
PRIMER_LEFT_PENALTY=0.000742
PRIMER_RIGHT_PENALTY=0.069737
PRIMER_LEFT_SEQUENCE=TAGTCGTCCGAAATCAAAACCT
PRIMER_RIGHT_SEQUENCE=CACTGACTCAATTGGTGGTGTT
PRIMER_LEFT=1401,22
PRIMER_RIGHT=1659,22
PRIMER_LEFT_TM=60.001
PRIMER_RIGHT_TM=59.930
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=259
PRIMER_PAIR_PENALTY_1=0.1903
PRIMER_LEFT_1_PENALTY=0.000742
PRIMER_RIGHT_1_PENALTY=0.189596
PRIMER_LEFT_1_SEQUENCE=TAGTCGTCCGAAATCAAAACCT
PRIMER_RIGHT_1_SEQUENCE=TGTTGTTTTTGAAGCCACTGAC
PRIMER_LEFT_1=1401,22
PRIMER_RIGHT_1=1674,22
PRIMER_LEFT_1_TM=60.001
PRIMER_RIGHT_1_TM=60.190
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=274
PRIMER_PAIR_PENALTY_2=0.2106
PRIMER_LEFT_2_PENALTY=0.140832
PRIMER_RIGHT_2_PENALTY=0.069737
PRIMER_LEFT_2_SEQUENCE=GGATTTCCACTGTCACGATGTA
PRIMER_RIGHT_2_SEQUENCE=CACTGACTCAATTGGTGGTGTT
PRIMER_LEFT_2=1381,22
PRIMER_RIGHT_2=1659,22
PRIMER_LEFT_2_TM=59.859
PRIMER_RIGHT_2_TM=59.930
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.3304
PRIMER_LEFT_3_PENALTY=0.140832
PRIMER_RIGHT_3_PENALTY=0.189596
PRIMER_LEFT_3_SEQUENCE=GGATTTCCACTGTCACGATGTA
PRIMER_RIGHT_3_SEQUENCE=TGTTGTTTTTGAAGCCACTGAC
PRIMER_LEFT_3=1381,22
PRIMER_RIGHT_3=1674,22
PRIMER_LEFT_3_TM=59.859
PRIMER_RIGHT_3_TM=60.190
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=294
PRIMER_PAIR_PENALTY_4=0.4251
PRIMER_LEFT_4_PENALTY=0.000742
PRIMER_RIGHT_4_PENALTY=0.424392
PRIMER_LEFT_4_SEQUENCE=TAGTCGTCCGAAATCAAAACCT
PRIMER_RIGHT_4_SEQUENCE=ACAACCCTGTTGTTTTTGAAGC
PRIMER_LEFT_4=1401,22
PRIMER_RIGHT_4=1681,22
PRIMER_LEFT_4_TM=60.001
PRIMER_RIGHT_4_TM=60.424
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_06243 Ps_contig_mira-and-tgicl-ass_franssen_42503
SEQUENCE=AAGCAGTGGTTATTCAACGCAGAGTACGTCGGGGGCTCTCTACCTCTCTCTCTTCACATCCAGCCGCTGTTCGAGGTGCGGCCAGTCACTCATTCAGAATGGTTTTTGTCAAGTCTCAGAAATCAAAGGCTTACTTCAAGAGGTTTCAAGTCAAGTTCAAGAGAAGAAGAGAGGGTAAGACTGATTACAGAGCCAGGATTCGTTTGATTAATCAGGACAAGAACAAATACAATACTCCCAAGTATCGTTTTGTTGTTCGATTTACAAACAAAGACATTGTTGCTCAAATTGTCTCTGCTAGCATTGCTGGTGATATTGTTCTTGCTGCCGCTTATGCGCATGAACTTCCTCACTATGGGCTTGAAGTAGGTCTTACCAACTATGCTGCAGCCTATTGCACCGGTCTCCTGTTGGCTCGCCGTGTTCTTAAAACTCTTGAAATGGATGAGGAGTATGAGGGCAATGTAGAGGCTAGTGGAGAAGATTATTCAGTTGAACCAGCTGATTCCAGGAGGCCTTTCCGTGCTCTTCTTGATGTTGGTTTGGTTAAGACCACAACCGGTAACCGTGTGTTTGGTGCACTCAAGGGAGCTTTGGATGGAGGTTTGGATATTCCTCACAGTGACAAAAGGTTTGCTGGTTTTGACAAGGAGAAGAAGGAGCTTGATGCTGAGGTTCACAGGAAGTATATCTTTGGTGGACATGTTACCGCCTATATGAAGACATTGATTGAAGACGAGCCAGAAAAATAATCAAACTCACTTCAGTCAATATATCAAGAAAGGGATAGAAGCTGATGGAATTGAGGAACTTTACAAGAAGGTTCATGCTGCAATTCGTGCAGACCCCTCAATCAAAAAGGCCGAGAAGCAGCCACCAAAGGAACACAAGAGGTACAACCTGAAGAAGCTCACTTATGATGAAAGAAGGACAAAGTTGATTGCTCGCTTGGAGGCACTAAATTCTGCAGTGGATGAAGACGAAGACGACGATGAGTGAAATTAAGTTGAGACCGGAGTCTATTGTTAGCTTGAATTTTCATTTTCATTTCCTTAAAATTTTTGTTGCTTGTACCTTTAATTTTAGATCAACATTTTTGGGTAATTTGGATTATCCTAGTTTGAGTTGTTTCAATTTACATGAATTGTTTCTGAAAGTAATTTCTTGCAGTTGAATCAACTTAAGTTATACNANTTANTRNNAGTYATCATACTTTGTTAGAGCTGAAAAATTATATTAACTTATAGTCCTACAAAAAGAAGTGGTCTGAATGATCAATTTTGGGTTTTTTTGTTTCATCAAAAAAAAAA
TARGET=44,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06244 Ps_contig_mira-and-tgicl-ass_franssen_42507
SEQUENCE=TCGAGAGGGAAAATAAACAGAAGGAGAAAGAAAAAATGGCCAAGAATCAGCAGCAAATTTCCATGCCAGGTTTGAAAAAGGGTTCGTCCACATTCTTGAGACCAAAAAGGTTTGGTTTGTAAATATATTATAACACTGTCAGATATTAGTGAACTCATGGGAGAGAGGGAAGTGAAAAAAGGCCTACATTCCAATTTTAAGGAGTAATCGGCAAATTACAAATTATTATTATTGATTTGTTACTGGAGTTATTTCCTCTGGCCATACTTCCTGCTCCTCAAGTTACTGTTATGTATATGTTGCATTGACTGATAGAAGAAATTCACCAGTTATCAAATTGCCATCTATTTAGAACTTGTAATATAGTGTTCAGCAGTCATGCATTTATCTTTCAGCCTTTTTCTGTACAAAACACCAAGCGATTAGTACTTAAAAAGATATTTGTTAGTAAACTTTTCTTCTGCACAATAGAAATTAATTTATTTAGATAAGTGCATCAGACTTATCTGTTGTATTAAGAAAAAGACATAGAATATTAAG
TARGET=222,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6816
PRIMER_LEFT_PENALTY=0.490741
PRIMER_RIGHT_PENALTY=0.190908
PRIMER_LEFT_SEQUENCE=AACTCATGGGAGAGAGGGAAGT
PRIMER_RIGHT_SEQUENCE=AAATGCATGACTGCTGAACACT
PRIMER_LEFT=151,22
PRIMER_RIGHT=385,22
PRIMER_LEFT_TM=60.491
PRIMER_RIGHT_TM=59.809
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=6.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=235
PRIMER_PAIR_PENALTY_1=0.7177
PRIMER_LEFT_1_PENALTY=0.490741
PRIMER_RIGHT_1_PENALTY=0.226971
PRIMER_LEFT_1_SEQUENCE=AACTCATGGGAGAGAGGGAAGT
PRIMER_RIGHT_1_SEQUENCE=ACTTGAGGAGCAGGAAGTATGG
PRIMER_LEFT_1=151,22
PRIMER_RIGHT_1=282,22
PRIMER_LEFT_1_TM=60.491
PRIMER_RIGHT_1_TM=59.773
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=0.8196
PRIMER_LEFT_2_PENALTY=0.490741
PRIMER_RIGHT_2_PENALTY=0.328860
PRIMER_LEFT_2_SEQUENCE=AACTCATGGGAGAGAGGGAAGT
PRIMER_RIGHT_2_SEQUENCE=AGGAAGTATGGCCAGAGGAAAT
PRIMER_LEFT_2=151,22
PRIMER_RIGHT_2=271,22
PRIMER_LEFT_2_TM=60.491
PRIMER_RIGHT_2_TM=60.329
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=121
PRIMER_PAIR_PENALTY_3=1.0275
PRIMER_LEFT_3_PENALTY=0.490741
PRIMER_RIGHT_3_PENALTY=0.536763
PRIMER_LEFT_3_SEQUENCE=AACTCATGGGAGAGAGGGAAGT
PRIMER_RIGHT_3_SEQUENCE=GGAAGTATGGCCAGAGGAAATA
PRIMER_LEFT_3=151,22
PRIMER_RIGHT_3=270,22
PRIMER_LEFT_3_TM=60.491
PRIMER_RIGHT_3_TM=59.463
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=120
PRIMER_PAIR_PENALTY_4=1.1482
PRIMER_LEFT_4_PENALTY=0.490741
PRIMER_RIGHT_4_PENALTY=0.657455
PRIMER_LEFT_4_SEQUENCE=AACTCATGGGAGAGAGGGAAGT
PRIMER_RIGHT_4_SEQUENCE=GATGGCAATTTGATAACTGGTG
PRIMER_LEFT_4=151,22
PRIMER_RIGHT_4=344,22
PRIMER_LEFT_4_TM=60.491
PRIMER_RIGHT_4_TM=59.343
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=194
=
PRIMER_SEQUENCE_ID=SSR_06245 Ps_contig_mira-and-tgicl-ass_franssen_42508
SEQUENCE=TCGAGAGGGAAAATAAACAGAAGGAGAAAGAAAAAATGGCCAAGAATCAGCAGCAAATTTCCATGCCAGGTTTGAAAAAGGGTTCGTCCACATTCTTGAGACCAAAAAGGTTTGGTTTGTAAATATATTATAACACTGTCAGATATTAGTGAACTCATGGGAGGAGAGGGAAGTAGAAAAAAGGCCTACATTACCAATTTTAAGGAGTAATCGGCAAATTACAATTATTATTATTGNTTTGTTACTGGAGTTTATTTCCTCTGG
TARGET=224,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.1227
PRIMER_LEFT_PENALTY=0.068602
PRIMER_RIGHT_PENALTY=3.054119
PRIMER_LEFT_SEQUENCE=GGGAGGAGAGGGAAGTAGAAAA
PRIMER_RIGHT_SEQUENCE=CCAGAGGAAATAAACTCCAGTAACA
PRIMER_LEFT=158,22
PRIMER_RIGHT=263,25
PRIMER_LEFT_TM=60.069
PRIMER_RIGHT_TM=59.946
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=106
PRIMER_PAIR_PENALTY_1=3.4924
PRIMER_LEFT_1_PENALTY=0.438317
PRIMER_RIGHT_1_PENALTY=3.054119
PRIMER_LEFT_1_SEQUENCE=ATGGGAGGAGAGGGAAGTAGAA
PRIMER_RIGHT_1_SEQUENCE=CCAGAGGAAATAAACTCCAGTAACA
PRIMER_LEFT_1=156,22
PRIMER_RIGHT_1=263,25
PRIMER_LEFT_1_TM=60.438
PRIMER_RIGHT_1_TM=59.946
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=108
PRIMER_PAIR_PENALTY_2=3.9767
PRIMER_LEFT_2_PENALTY=0.922573
PRIMER_RIGHT_2_PENALTY=3.054119
PRIMER_LEFT_2_SEQUENCE=GTTCGTCCACATTCTTGAGACC
PRIMER_RIGHT_2_SEQUENCE=CCAGAGGAAATAAACTCCAGTAACA
PRIMER_LEFT_2=81,22
PRIMER_RIGHT_2=263,25
PRIMER_LEFT_2_TM=60.923
PRIMER_RIGHT_2_TM=59.946
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=183
PRIMER_PAIR_PENALTY_3=3.9767
PRIMER_LEFT_3_PENALTY=0.922573
PRIMER_RIGHT_3_PENALTY=3.054119
PRIMER_LEFT_3_SEQUENCE=GGTTCGTCCACATTCTTGAGAC
PRIMER_RIGHT_3_SEQUENCE=CCAGAGGAAATAAACTCCAGTAACA
PRIMER_LEFT_3=80,22
PRIMER_RIGHT_3=263,25
PRIMER_LEFT_3_TM=60.923
PRIMER_RIGHT_3_TM=59.946
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.1000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=184
PRIMER_PAIR_PENALTY_4=3.9775
PRIMER_LEFT_4_PENALTY=0.923358
PRIMER_RIGHT_4_PENALTY=3.054119
PRIMER_LEFT_4_SEQUENCE=GTCCACATTCTTGAGACCAAAA
PRIMER_RIGHT_4_SEQUENCE=CCAGAGGAAATAAACTCCAGTAACA
PRIMER_LEFT_4=85,22
PRIMER_RIGHT_4=263,25
PRIMER_LEFT_4_TM=59.077
PRIMER_RIGHT_4_TM=59.946
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_06246 Ps_contig_mira-and-tgicl-ass_franssen_42510
SEQUENCE=GACAATTGCAATCCTAATTCCTAGAAAAATTACTTTTATTCTCTCTCTCTAACTTTCTCTAACTACTTTCATTCTTGTTTGACTGTGTTGTTTCAAAAGGGTAATCCCAATGGCAAAAGACGTTGAAGTTGCTGAACGTGGCTCTTTCTCCAACAAAGACTACCATGACCCTCCTCCAGCACCACTCATTGACGCTGAGGAACTCACAAAATGGTCCTTTTACAGGGCCCTTATTGCTGAGTTCATTGCAACTTTACTTTTCCTTTACGTTACTGTTTTGACTGTGATTGGTTACAGTATCCAAACTGATGTTAAAGCTGGTGGTGATGAGTGCGGTGGTGTTGGTATTCTTGGAATTGCTTGGGCTTTTGGTGGCATGATCTTTGTTCTTGTTTACTGCACTGCTGGGATTTCAGGGGGTCACATTAACCCGGCAGTGACGTTTGGGCTATTTTTGGCTCGTAAGGTGTCTTTGATAAGAGCGGTTATGTACATGGTGGCTCAGAGTTTGGGGGCTATTTGTGGAGTTGGGTTGGTTAAGGCTTTTCAGAAGTCTTACTTTGTTAGATATGGTGGTGGAGCTAATTTTCTTCAACCTGGTTATAGTACTGGTGTTGGATTAGGTGCTGAGATTGTTGGTACCTTTGTTTTAGTTTACACCGTTTTCTCTGCTACTGATCCTAAGAGAAGTGCTAGAGATTCTCATGTGCCGGTTTTGGCTCCACTTCCCATTGGATTTGCTGTATTCATGGTTCATTTGGCAACCATCCCAGTCACCGGAACCGGCATTAATCCCGCCAGAAGTCTTGGTTCTGCTGTTATCCTTAACCAAGATAAGATTTGGAATGACCATTGGATATTTTGGGTTGGACCATTTATCGGGGCCGCCATTGCAGCGTTCTACCATCAATTCATCTTAAGAGCTGGTGCTGCTAAAGCTCTTGGATCATTCAGGAGTAGCAATGCTAATTGAGTGCTAATGGAAAGAAGATGAAGTGATTGATACTATTTGCTTTGTATTTGCAAGTTTGCAACCTCTCTCTCAATTGTGTAATCAAGATTTGTGGATAAGTGTTAGGTTAAAAAGTCTCTTTCGTTACAGAGGGAGCCTTTTTACACTTTCCTCTGTATTCTCTTTAGTGTGTCTTCTTTTTCTTTTTTCTAAGTTATTCTCTTCTGTAATCCTTTGTACTCATTGAATATTTATGGAAAAAATTTCAAGTTTATTGAAGATAAATATTATAGTGCACATTTATACC
TARGET=40,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06247 Ps_contig_mira-and-tgicl-ass_franssen_42514
SEQUENCE=AATTTTTTGTTAATACAAAGTAATATAAATCAAACAAATAATCCACATAGATGTCATGTTCTAAAAATAATATAGATNNNTTGTNCACAAGTAAACATTCTACTTAAACGACATGTTTTAAGCAATTCACTCAAGCAAACAAATGTTGCGGCAAAGTAAATAGATTACTAGACATTTCGAAAGAAACAACCCACTTGGACTTCATGTGGTTAAAAATCTACTGTAGCAGCCATTTTATGAAACAGCTTTCAAAACAACTAAAGTTGCTGTATACTAGTGTGATTTCTGAGTAGAAAAAGATATACACGTCAAAAAAAGTTTGAGGGTGGGGGTGGGGGGACAAAACTTAAAACAAGGGTTATGCCCCAGTGGTAAGCAATCACAAAAATGGAACTTTCCTTGTTTAACAGGGTATTTTCACAGATTATTTCCCGCATACAATTGCTCCATCCCAATTCTCAGCACTACACAAGGTTCATTTTCTTCCGCATTAAACATCTCTCTAGAGCATGGCTCATCAGAGGACAACAGACACCCATTGTAGGACAAAAACTGGGGCTGCTGAACTCAAATTCCAGTAGCAGGTCTTCGTTTCACAACCAAATCATCAGCCGAATAGTAATCAGCAATGAGCCGGTACATGTATGATGGGTTATGCCCTCCAGTGTCAGTAATAAAGAGACTAACAAGTTTTGGTTGGCACATAATCGAACGTAGGGTTTACGACATGGAGAAAACCTCCACCACTTGCAGAATCCATGCAATCCGAGAACTCCCCAAAGTCAAGTAGCTCAGACGGGGATCTCAGCTCGTTTAATAGGACTTCAGGATTGTGTGGATACAAAGGGCACAACTTGTGACTTCCAGCAAGTACAACAAAAGGAACAGCATGCCTTTGAGCTGCAAGTGCAACCATATTTAATCCAACTGGGGCAATGACTCCACCATTAGCCATGACAGCATGAGCTCCAACTATAACCATGTTCACTCGGGAAATCATGGCAAAAACTGCAGAATCAGTAATCACTGTGGTCTGTAAGCCTCTAGCAGCCAACTCTTTTGCAAGGAGATGTCCCTGATATCTTGGGGCACCTTCGGCAACAAAGACCTTAAATGATCTCTTTTTCTCCTTTGCAGCACAAAGAAATTCCAACACTGTCTTTGAACTTCCTAGCGTTAATATTACCTCATTTTGATGAATGTGTTCTACTGCTTGTTCAGCTATTTGTTCATAGCAAGTAGATATGTCTTGAATAAGTTCATTAACTGCTTCAATGACATCGTGCTTCAACTTCCGACCTCTTGAACTCTTATCAATAGATCTACTTTTCCCTTCAGAATCACCCCCTGAAGAAGATGTGTGTGGAATGGCAGCTGTAATAGGTGTATCCTCTAGAAGAGTTTGCAATGATGGTGGACGCAAGGTGCTTCTTGCAGCAGCTGCAACAGCAGCAGCAGATAAAACAGGATAATCATCTCGATCCACATCATCTTCATCGTCGCTTACAGCTGATAGCTCCAAACCAGCTATAGCAGCTGTGGCAAGGGAAAGATCCTCCTCCCTTATAATGTGCAAAACTCGCCTCGCAATATTTCCCACAGCAAGCTCAACAGGATTAGCAGCGATAAGCTTCTCCCCGACAGCCCTTACTGCATTAATCAGAGCTGTGGCCTGAATTTGTCTGAGGCAACCTCTGTTGTGAAATCACAGACCGAAGCAACTCTGCTGTCTGCTTTGCCGAGGCCTGAGAACCTTCAATTTTACGTTTTTTAAGCTGATTCACAAAATCATTAACAAGGATATGAACATCCGGCATTTTGGTTCCAACCTGGATCTTGGCCTTGACGTTGTCGATGGTGTCGCTGCTCTCAACCTCGAGAGTGATGGTTTTTCCGGTTAAAGTCTTGACGAAGATCTGCATGTTGTTCCTCTTCTCATGGTTTTTGACGCAGTGAGAGCCCGAGAGAAAGAGGGACGCGAGAGGTGAAGAGAGAGACTGTGTTTGTGTTTAGTTTGAGTCCCCGCGNACACGGTGTGTGAGGGTATTTGAAGCTACTAGCCTGTAGCGGGATTCCGTTCGAGTATTCTTTTTCCATTTGTGTCGTGCGTAATCGTTAATATAGAATAAACAAGTTTATTCT
TARGET=1447,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1782
PRIMER_LEFT_PENALTY=0.148509
PRIMER_RIGHT_PENALTY=0.029729
PRIMER_LEFT_SEQUENCE=CCTGAAGAAGATGTGTGTGGAA
PRIMER_RIGHT_SEQUENCE=AAGCTTATCGCTGCTAATCCTG
PRIMER_LEFT=1346,22
PRIMER_RIGHT=1632,22
PRIMER_LEFT_TM=60.149
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.5000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=287
PRIMER_PAIR_PENALTY_1=0.1816
PRIMER_LEFT_1_PENALTY=0.151911
PRIMER_RIGHT_1_PENALTY=0.029729
PRIMER_LEFT_1_SEQUENCE=TGTGGAATGGCAGCTGTAATAG
PRIMER_RIGHT_1_SEQUENCE=AAGCTTATCGCTGCTAATCCTG
PRIMER_LEFT_1=1361,22
PRIMER_RIGHT_1=1632,22
PRIMER_LEFT_1_TM=60.152
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.2089
PRIMER_LEFT_2_PENALTY=0.148509
PRIMER_RIGHT_2_PENALTY=0.060408
PRIMER_LEFT_2_SEQUENCE=CCTGAAGAAGATGTGTGTGGAA
PRIMER_RIGHT_2_SEQUENCE=CTATCAGCTGTAAGCGACGATG
PRIMER_LEFT_2=1346,22
PRIMER_RIGHT_2=1515,22
PRIMER_LEFT_2_TM=60.149
PRIMER_RIGHT_2_TM=60.060
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=8.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=170
PRIMER_PAIR_PENALTY_3=0.2103
PRIMER_LEFT_3_PENALTY=0.148509
PRIMER_RIGHT_3_PENALTY=0.061818
PRIMER_LEFT_3_SEQUENCE=CCTGAAGAAGATGTGTGTGGAA
PRIMER_RIGHT_3_SEQUENCE=AGCTGCTATAGCTGGTTTGGAG
PRIMER_LEFT_3=1346,22
PRIMER_RIGHT_3=1537,22
PRIMER_LEFT_3_TM=60.149
PRIMER_RIGHT_3_TM=60.062
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=192
PRIMER_PAIR_PENALTY_4=0.2123
PRIMER_LEFT_4_PENALTY=0.151911
PRIMER_RIGHT_4_PENALTY=0.060408
PRIMER_LEFT_4_SEQUENCE=TGTGGAATGGCAGCTGTAATAG
PRIMER_RIGHT_4_SEQUENCE=CTATCAGCTGTAAGCGACGATG
PRIMER_LEFT_4=1361,22
PRIMER_RIGHT_4=1515,22
PRIMER_LEFT_4_TM=60.152
PRIMER_RIGHT_4_TM=60.060
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=8.6000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_06248 Ps_contig_mira-and-tgicl-ass_franssen_42523
SEQUENCE=GATTGCCGAATGGGGTTGGTTGGTGTTGGTGTGAGTCTTCACTTCGCTTGTGCCTCTTTCTCTCAATCTATCTTCCCACGCGATCTGTGATTGTCACGAAACAATAGAGATGATGTATTCGCGGTTTAGAACCGTCGCCGGAAATCTCAATTGCGCCGCGAAACGTCTCTCTTCATCTTCCACCACCACCACAACCACCTCAGCTCCGTCTGAATTACAGCAGAACCTAGCAGCGCTCCGGGCTCGGCTGGCGATGGAGTCGCCATCTCTGTCGGACTTCATTTCCTTGAAATCGGACAACGCCTACTCTGTTGAGGTTGGGACAAAGAAGAAACCTCTTCCGAAACCGAAATGGATGAAGGAATCAATTCCCGGTGGTGAGAAGTATGTGCAGATTAAAAAAAAGCTCCGTGAGTTGAAGCTTCACACGGTTTGTGAAGAGGCAAAGTGCCCTAATTTGGGAGAGTGTTGGTCAGGTGGTGAAACTGGCACTGCCACAGCTACTATCATGATTCTCGGAGATACTTGTACTCGAGGTTGCAGATTTTGCAATGTTAAGACATCAAGGACTCCTCCACCGCCTGACCCTGATGACGCCCACCAATGTGGCTGAGGCAATTGCATCATGGGGTTTGGATTATGTGGTTATAACAAGTGTTGACCGTGATGATTTACCTGATCAAGGGAGTGGTCATTTTACTGAAACAGTGCAGAAGCTGAAGGCACTGAAACCTAGCACGCTGATAGAAGCCTTAGTTCCCGATTTTCGAGGAAATGCTGAATGTGTAGAGAAGGTTTCCAAATCAGGACTAGATGTCTTTGCACATAATATTGAGACAGTTGAAGAGCTTCAGAGTGCTGTGAGGGATCACCGGGCTAATTTTAAGCAGTCTTTGGATGTTCTGATGATGGCGAAGGAGTATGCCCCTGCGGGGACACTCACCAAGACTTCAATAATGTTGGGTTGTGGGGAAACACCTGACCAGATTGTGAAGACAATGGAGAAGGTGAGAGCAGCTGGTGTTGATGTGATGACATTTGGTCAGTATATGAGACCCTCAAAGCGCCATATGCCTGTATCAGAATATATTACCCCTGAGGCCTTTGAGAAGTATCAGACTCTTGGCATGGAAATGGGATTTCGCTATGTGGCATCTGGACCCATGGTAAGGTCATCATACAAGGCAGGTGAATTCTATATTAAATCCATGATTGACTCAGATCGGGCTGCATCTTCCTAAAAGCTCTGTTGATCCATTTATTTTTTTTCTTTCATTTTTGTTATTTACACTCATGTGCATTATGTATAAGAGGTACATGTTTGCTTCATTCTGTTGATGCCCATACCATGTGTAATCTATCATTAAAATGGTATTTAATGGGCTCATAAATTCATTTGAAAAATGCAAATCTTAAAAGGTTAATCTGTGTTCTCATCTACCTGAACTCATTTTTTTCTCTTTAATCCATGCAGTTTACACTCATTGTTGTATTTATGAAAAGTAATTAGTTTGAAGGCTATTAATTGAGAAATCATGTAAAACTGTTACTTTTGAAGTGGTTAAATTTAAGTGTTTATTTACGT
TARGET=180,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0381
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.031122
PRIMER_LEFT_SEQUENCE=AGAGATGATGTATTCGCGGTTT
PRIMER_RIGHT_SEQUENCE=GTCCCAACCTCAACAGAGTAGG
PRIMER_LEFT=105,22
PRIMER_RIGHT=323,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=60.031
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=219
PRIMER_PAIR_PENALTY_1=0.0567
PRIMER_LEFT_1_PENALTY=0.025566
PRIMER_RIGHT_1_PENALTY=0.031122
PRIMER_LEFT_1_SEQUENCE=GCTTGTGCCTCTTTCTCTCAAT
PRIMER_RIGHT_1_SEQUENCE=GTCCCAACCTCAACAGAGTAGG
PRIMER_LEFT_1=45,22
PRIMER_RIGHT_1=323,22
PRIMER_LEFT_1_TM=60.026
PRIMER_RIGHT_1_TM=60.031
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.0619
PRIMER_LEFT_2_PENALTY=0.006951
PRIMER_RIGHT_2_PENALTY=0.054900
PRIMER_LEFT_2_SEQUENCE=AGAGATGATGTATTCGCGGTTT
PRIMER_RIGHT_2_SEQUENCE=GATTTCAAGGAAATGAAGTCCG
PRIMER_LEFT_2=105,22
PRIMER_RIGHT_2=293,22
PRIMER_LEFT_2_TM=59.993
PRIMER_RIGHT_2_TM=59.945
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=189
PRIMER_PAIR_PENALTY_3=0.0619
PRIMER_LEFT_3_PENALTY=0.006951
PRIMER_RIGHT_3_PENALTY=0.054900
PRIMER_LEFT_3_SEQUENCE=AGAGATGATGTATTCGCGGTTT
PRIMER_RIGHT_3_SEQUENCE=CGATTTCAAGGAAATGAAGTCC
PRIMER_LEFT_3=105,22
PRIMER_RIGHT_3=294,22
PRIMER_LEFT_3_TM=59.993
PRIMER_RIGHT_3_TM=59.945
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=190
PRIMER_PAIR_PENALTY_4=0.0619
PRIMER_LEFT_4_PENALTY=0.006951
PRIMER_RIGHT_4_PENALTY=0.054900
PRIMER_LEFT_4_SEQUENCE=AGAGATGATGTATTCGCGGTTT
PRIMER_RIGHT_4_SEQUENCE=GTCCGATTTCAAGGAAATGAAG
PRIMER_LEFT_4=105,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=59.993
PRIMER_RIGHT_4_TM=59.945
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=193
=
PRIMER_SEQUENCE_ID=SSR_06249 Ps_contig_mira-and-tgicl-ass_franssen_42528
SEQUENCE=TTCATAATTTAAGTAGTAGTACATCATGTATTGNTCAACTGTAACACGCAATTTCTTCTTCAGTACATTAATACAACATTACTACAAATCATTAATGCATATACCAGGACACATCAATTACATCACAATATTATTAACTTACAAACATTCAGAGAGTATACAATACACGCATTATGAGCACTGCATACTATAATCACATCACAAGTTTATTAGTCATTCACACACAAAATATAAAGCACATGAACAACACATGCGCACAAAAATATAGCATCCCAACGCTCCAATGTGCACGCCGGCATACCGATCTCAAACTGCAACAGCCCTGCATAAATATCCTTTCGAAGAACAAACAAAACTTGAAACAACATCAGCAGAAAGAGCATACACATAGGACCATAAGCATGAAAAAAAGGTATTCAAACAGAAACGAAATGCATAATGAATGAGCATTATAATCAACTTATTTCTGAAATGAAATCTGAGTAGCTAACAATACGGCCAATCTCGATCCGTTACGGTCAGTGGGAAACTGCTATTGTAGTGTTGATCTTGCCACTGTTGTGTCGGTCAACGCCTTGTGACTGGATTCGCTCTTCGAACTCGCCATAACTTCCATCAACTCTGGAGCCGGTAACCTCAATCCTTCAACTAAACCAGAACCTTTGGCAAGGAACTGATCTGCAGAACTGATGAACTTTGCTTTGCATATTGCAGCGGTGACGGTTAATGTTGTAGCCAAAATTGATTCTGAGCTTCTTCGCCGTTGACGGTTGGGTTCGTAGTGTCCGAGGTCCCTCCATCTGCACTTATATTTTCTTCGTGAATCTTCGTCGGATGTGGTTCCACCTGTAAAAAAACAACAGTTTCCAACAACAACAAACATTTCACAGTAATGGCGATCAAAATGATTCGCCATGAAAGTCCCCGCACCACACTCCGCAGTAGGGCACTCCTTCCTCAACCTCTGCACCTTTCCAGAATCATCCATCTTGTAGAACTGGAGAACCGCAAGCTTCACCTTCCTGTGCTTATGCTTGATTCTTTCGTTAGGGTCGTTTGGGTTGGTTACGGGTTTCCTTTTCCTTTCCTTCGTTGGAACGAACCAACCGGACCGAAACGGAAGGGGGGAATAGGGACCGAAAGGGCCAACGAACGGAGGGGGA
TARGET=868,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3833
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.376347
PRIMER_LEFT_SEQUENCE=AATTGATTCTGAGCTTCTTCGC
PRIMER_RIGHT_SEQUENCE=CATTTTGATCGCCATTACTGTG
PRIMER_LEFT=739,22
PRIMER_RIGHT=906,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=60.376
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.4114
PRIMER_LEFT_1_PENALTY=0.035021
PRIMER_RIGHT_1_PENALTY=0.376347
PRIMER_LEFT_1_SEQUENCE=CAACTAAACCAGAACCTTTGGC
PRIMER_RIGHT_1_SEQUENCE=CATTTTGATCGCCATTACTGTG
PRIMER_LEFT_1=645,22
PRIMER_RIGHT_1=906,22
PRIMER_LEFT_1_TM=60.035
PRIMER_RIGHT_1_TM=60.376
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=262
PRIMER_PAIR_PENALTY_2=0.4114
PRIMER_LEFT_2_PENALTY=0.035021
PRIMER_RIGHT_2_PENALTY=0.376347
PRIMER_LEFT_2_SEQUENCE=AACTAAACCAGAACCTTTGGCA
PRIMER_RIGHT_2_SEQUENCE=CATTTTGATCGCCATTACTGTG
PRIMER_LEFT_2=646,22
PRIMER_RIGHT_2=906,22
PRIMER_LEFT_2_TM=60.035
PRIMER_RIGHT_2_TM=60.376
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.4357
PRIMER_LEFT_3_PENALTY=0.059316
PRIMER_RIGHT_3_PENALTY=0.376347
PRIMER_LEFT_3_SEQUENCE=TGCAGAACTGATGAACTTTGCT
PRIMER_RIGHT_3_SEQUENCE=CATTTTGATCGCCATTACTGTG
PRIMER_LEFT_3=679,22
PRIMER_RIGHT_3=906,22
PRIMER_LEFT_3_TM=60.059
PRIMER_RIGHT_3_TM=60.376
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=228
PRIMER_PAIR_PENALTY_4=0.4502
PRIMER_LEFT_4_PENALTY=0.073900
PRIMER_RIGHT_4_PENALTY=0.376347
PRIMER_LEFT_4_SEQUENCE=GTGACGGTTAATGTTGTAGCCA
PRIMER_RIGHT_4_SEQUENCE=CATTTTGATCGCCATTACTGTG
PRIMER_LEFT_4=716,22
PRIMER_RIGHT_4=906,22
PRIMER_LEFT_4_TM=59.926
PRIMER_RIGHT_4_TM=60.376
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=191
=
PRIMER_SEQUENCE_ID=SSR_06250 Ps_contig_mira-and-tgicl-ass_franssen_42530
SEQUENCE=GTCGGNACACATTAATATACATTCAGTCTCTTCTAGTAATACGCGACACGATAACTCCATACAAATTACAACCTCTCTCTCTAAGCTTCAAAGAAACTCACTCTCCCATGGCGACTTCTTTCACGCCACTATCCATTTCAGTTGGTGGATCTCATCTCAAGTCATCTGAGCTGTTTTTAGCTAAGCGTAATTCATCTCTGGTTGGACCTAACCTTGCTGTTCAAAGGAAGTCAAACATTGTGAGCAGAAAGAACCTCATTTCACCACTTCGTGCAGAATACCGTGATAACAGAGGAGGTGGAGGTGGAGGTGGGGAATTGCTTACTGGCTTTCTTCTGGGTGGTGCTATTTTTGGAACCCTGGCTTATGTTTTTGCTCCCCAGATCAGAAGAACTTGGCTAAATGAAAATGAACATGGGTTTCAGAAGGCCAGAAGACCAATATATTATGACGAAGGGACAAGACCAATGCTTTATGACGAAGGGCTAGAGATAACCAAGGAGGTCTTGAATGAAAAAATAGGCCAGCTAAACTCAGCCATTGATAATATATATTCACGTCTGAGAGGCAACAACAATAAAGTGCCTGTCCCTAAAGAAAGTGATCCTGAAATTGAAGCGATATAATTATCAAACTAGCTGTAACCTGTTTATTTTCTTACGACTGGAATCAGGCTACTGTTTAGCCCCTTTGTTACTTGGATTTCACTTACAAGAAACCTGACTTCTACTTCTATTGAATAATTTTGATATACTTTTAAAATTTATTCTCCAGTTAAAGAGTTTAAGGTGCAAAATAGATTTACATATGGGAATCTATCATCTTGCCATGTAGGTATATTTTGTAATTGTCTTTTAAAAATTAAATGCAGACATATTTTGTTTTGTTTTTACTGAAT
TARGET=73,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0302
PRIMER_LEFT_PENALTY=0.024665
PRIMER_RIGHT_PENALTY=0.005574
PRIMER_LEFT_SEQUENCE=GCGACACGATAACTCCATACAA
PRIMER_RIGHT_SEQUENCE=CACCTCCACCTCCTCTGTTATC
PRIMER_LEFT=42,22
PRIMER_RIGHT=305,22
PRIMER_LEFT_TM=60.025
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.1357
PRIMER_LEFT_1_PENALTY=0.024665
PRIMER_RIGHT_1_PENALTY=0.111077
PRIMER_LEFT_1_SEQUENCE=GCGACACGATAACTCCATACAA
PRIMER_RIGHT_1_SEQUENCE=TTGAGATGAGATCCACCAACTG
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=159,22
PRIMER_LEFT_1_TM=60.025
PRIMER_RIGHT_1_TM=60.111
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=0.1412
PRIMER_LEFT_2_PENALTY=0.024665
PRIMER_RIGHT_2_PENALTY=0.116582
PRIMER_LEFT_2_SEQUENCE=GCGACACGATAACTCCATACAA
PRIMER_RIGHT_2_SEQUENCE=GGTGAAATGAGGTTCTTTCTGC
PRIMER_LEFT_2=42,22
PRIMER_RIGHT_2=264,22
PRIMER_LEFT_2_TM=60.025
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=223
PRIMER_PAIR_PENALTY_3=0.2445
PRIMER_LEFT_3_PENALTY=0.024665
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=GCGACACGATAACTCCATACAA
PRIMER_RIGHT_3_SEQUENCE=TGCTCACAATGTTTGACTTCCT
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=245,22
PRIMER_LEFT_3_TM=60.025
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.0000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.2445
PRIMER_LEFT_4_PENALTY=0.024665
PRIMER_RIGHT_4_PENALTY=0.219884
PRIMER_LEFT_4_SEQUENCE=GCGACACGATAACTCCATACAA
PRIMER_RIGHT_4_SEQUENCE=CTGCTCACAATGTTTGACTTCC
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=60.025
PRIMER_RIGHT_4_TM=59.780
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=205
=
PRIMER_SEQUENCE_ID=SSR_06251 Ps_contig_mira-and-tgicl-ass_franssen_42532
SEQUENCE=GATTCCATTTGTTGGNAAGGCATTTGTCCTATATATATTACCAACAGCAAACAAATGARNGGAAAAACAAAGATGATATCTTCAACCATGCAACAACACTTCTCATGCACACTCCTCCTCCTCCTAATCCTAACAACACATCACTTCATACCTTCCCTCTCAGAAAAATGTAACCCACACGACAAAAACGACCCTTCTCCAAATCAAAAAAGAACTCGGCAACCCAACCAAACTCTCCTCATGGAACCCAACCACCGACTGCTGCAACGGCAAATGGCTCGGTGTCGCATGCGACACCGACACACAAACCTACCGCGTAACCAACCTTGACCTCGACGAACTCGACCTTCTTAAACCGCTTCCGACTCCACCCTCCATTCCCAACCTCCCGTTCCTCTCTTTCCTCACTCTCTCCCGTACCCCGAACCTCTCCGGCCCTATCCCTCCCTCCCTCGCCAACCTCACCAAACTCCAGTATCTCTACATCATGCAAACCGGCATCTCCGGCGAGATACCCAACACTCTCTCACAGATCAAAACCCTCTTAACCATCGACTTAACCAACAACAAACTCACCGGTCCTCTCCCCGCCTCACTCTCCTCTCTCCCTAAACTCATCGGAATCTCCTTCGACGGCAACCAACTCACAGGTACAATTCCAGAATCATACGGCTCGTTCCCGAAGTCATTCACAGTGCTAACTCTCTCAAAAAACCGCCTCTCGGGAAAAATCCCAGCATCTCTGGCGAAACTGAACCTTGCGTTTGTTGACTTGGAACGGAACGCTTTGGAGGGTGATGCTTCTGTGTTTTTTGGGTCGAAGAAAAGCACTCAGAAGATATTGCTGGGAAAGAACTCTTTAGCTTTTGATATTGGGAAAGTTGGGTTGTCGAAGGATTTGAATGCGTTGGACTTGAGGAACAATAAAATCTATGGTACGCTACCTGTGGGACTAACGAAGCTGAAGTTTTTGAGCAAGTTCAATGTGAGTAACAATAATTTATGTGGAAAGATTCCACAAGGTGGAAAGTTGCAGAGGTTTGATGAATCTTCTTATGCTCATAATCAATGCTTGTGTGGATCTCCTCTTGGAGGTTGCAAAGCTTGAGGAGAAGAATATCACTGAACTATTGCAGTTTTAATAAAATAGATGAGAGTGTAGGTTTAAATTTAAAGCCACATATTGTATTGCGTTGAATGTTGATGTAATTTTGGACAATTAATTACTGATCCCTCCATCTTATATATTATGTGATCCTAAATTAACTACAAAGTGTAGCTTTTAATGGAAAATATTTTTACAGCTTCCTG
TARGET=112,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06252 Ps_contig_mira-and-tgicl-ass_franssen_42532
SEQUENCE=GATTCCATTTGTTGGNAAGGCATTTGTCCTATATATATTACCAACAGCAAACAAATGARNGGAAAAACAAAGATGATATCTTCAACCATGCAACAACACTTCTCATGCACACTCCTCCTCCTCCTAATCCTAACAACACATCACTTCATACCTTCCCTCTCAGAAAAATGTAACCCACACGACAAAAACGACCCTTCTCCAAATCAAAAAAGAACTCGGCAACCCAACCAAACTCTCCTCATGGAACCCAACCACCGACTGCTGCAACGGCAAATGGCTCGGTGTCGCATGCGACACCGACACACAAACCTACCGCGTAACCAACCTTGACCTCGACGAACTCGACCTTCTTAAACCGCTTCCGACTCCACCCTCCATTCCCAACCTCCCGTTCCTCTCTTTCCTCACTCTCTCCCGTACCCCGAACCTCTCCGGCCCTATCCCTCCCTCCCTCGCCAACCTCACCAAACTCCAGTATCTCTACATCATGCAAACCGGCATCTCCGGCGAGATACCCAACACTCTCTCACAGATCAAAACCCTCTTAACCATCGACTTAACCAACAACAAACTCACCGGTCCTCTCCCCGCCTCACTCTCCTCTCTCCCTAAACTCATCGGAATCTCCTTCGACGGCAACCAACTCACAGGTACAATTCCAGAATCATACGGCTCGTTCCCGAAGTCATTCACAGTGCTAACTCTCTCAAAAAACCGCCTCTCGGGAAAAATCCCAGCATCTCTGGCGAAACTGAACCTTGCGTTTGTTGACTTGGAACGGAACGCTTTGGAGGGTGATGCTTCTGTGTTTTTTGGGTCGAAGAAAAGCACTCAGAAGATATTGCTGGGAAAGAACTCTTTAGCTTTTGATATTGGGAAAGTTGGGTTGTCGAAGGATTTGAATGCGTTGGACTTGAGGAACAATAAAATCTATGGTACGCTACCTGTGGGACTAACGAAGCTGAAGTTTTTGAGCAAGTTCAATGTGAGTAACAATAATTTATGTGGAAAGATTCCACAAGGTGGAAAGTTGCAGAGGTTTGATGAATCTTCTTATGCTCATAATCAATGCTTGTGTGGATCTCCTCTTGGAGGTTGCAAAGCTTGAGGAGAAGAATATCACTGAACTATTGCAGTTTTAATAAAATAGATGAGAGTGTAGGTTTAAATTTAAAGCCACATATTGTATTGCGTTGAATGTTGATGTAATTTTGGACAATTAATTACTGATCCCTCCATCTTATATATTATGTGATCCTAAATTAACTACAAAGTGTAGCTTTTAATGGAAAATATTTTTACAGCTTCCTG
TARGET=441,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06253 Ps_contig_mira-and-tgicl-ass_franssen_42533
SEQUENCE=GTTTTTTTTTTTAATATTGAGAACACAATTTAACTTTAAAGATTCGCATTTTCCATACATTTGTCCATTCATTGATGGTTTATTGCCCGTTTAATGATAGATTACATGCGGTAAGGGAGTCAATATAAAGTTAGCCTGCACCTCTTCTCATACATAATGCACAGTAGTGTGTAAATAACAAAAATGAAAGAAAAAATAATGGATCAACAGGTACTCAGCTTTGAGGAAGATGCGGCTCGATCTCTGTCAATCATGGATTTAATATAGAATTCACCTGCTTTGTATGATGACCTTACCATGGGCCCAGATGCCACATATCGAAATCCCATTTCCATGCCAAGAGTCTGATACTTCTCAAAGGCCTCGGGAGTTATATATTCTGACACTGGCATATGGCGCTTTGAGGGTCTCATATACTGACCAAATGTCATCACATCAACACCAGCTGCTCTCACTTTCTCCATTGTTTTCACAATCTGGTCAGGTGTTTCCCCACAACCTAACATTATTGAAGTCTTGGTGAGGGTCCCCGCAGGGGCGTAATCCTTAGCCATCATCAGAACATCCAAAGACTGCTTAAACTTAGCCCGATGATCCCGCACCGCACTTTGAAGCTCTTCAACTGTCTCGATATTGTGCGCAAAGACATCTAGTCCTGATTTAGAAACTTTCTCTACGCATTCAGCATTTCCTCGAAAATCGGGAACTAAGGCTTCTATCAACATGCTAGGTTTCAGTGCCTTCAGCTTCTGCACTGTTTCAGTAAAATGACCACTCCCTTGATCAGGTAAATCATCACGGTCAACACTTGTTATAACCACATAATCCAAACCCCACGACGCAATTGCCTCAGCCACATTGGTGGGCTCATCAGAGTCAGGTGGTGGAGGAGTTCTTGATGTCTTAACATTACAAAATCTGCAACCTCGAGTACAAGTATCTCCAAGAATCATGATAGTGGCAGTGGCAGTGCCGGTTTCTCCACCGGACCAACACTCTCCCAAATTAGGGCACTTTGCCTCCTCACAAACCGTATGAAGCTTCAATTCACGGAGCTTTTTCTTAATCTGCACATACTTCTCACCACCGGGGATTGATTCCTTCATCCATTTCGGTTTGGGAAGCGGTTTCTTCTTAGTTCCAACCTCAACAGAGTACGCTTTGTCCGATTTCAAGGAAATGAAGTCCGACAGAGATGGTGACTCGGTCGCTAGCCGAGCCCGAAGTCCTGCCAGATTCTGCTGGAATTCAGACGGAGCTGAGGTGGTTGTGGTGGTGGTGGAAGATGAAGAGAAGGTTTTTGTAGCGCACTTGAGGCTTCCGGCGACGGTTCTAAACCGCGAATAGATCATCTCTCTCGTTTCTTGACAATCACCGATTC
TARGET=1270,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1008
PRIMER_LEFT_PENALTY=0.054900
PRIMER_RIGHT_PENALTY=0.045851
PRIMER_LEFT_SEQUENCE=GATTTCAAGGAAATGAAGTCCG
PRIMER_RIGHT_SEQUENCE=GTGCGCTACAAAAACCTTCTCT
PRIMER_LEFT=1167,22
PRIMER_RIGHT=1310,22
PRIMER_LEFT_TM=59.945
PRIMER_RIGHT_TM=59.954
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=144
PRIMER_PAIR_PENALTY_1=0.1008
PRIMER_LEFT_1_PENALTY=0.054900
PRIMER_RIGHT_1_PENALTY=0.045851
PRIMER_LEFT_1_SEQUENCE=GATTTCAAGGAAATGAAGTCCG
PRIMER_RIGHT_1_SEQUENCE=AGTGCGCTACAAAAACCTTCTC
PRIMER_LEFT_1=1167,22
PRIMER_RIGHT_1=1311,22
PRIMER_LEFT_1_TM=59.945
PRIMER_RIGHT_1_TM=59.954
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=145
PRIMER_PAIR_PENALTY_2=0.1008
PRIMER_LEFT_2_PENALTY=0.054900
PRIMER_RIGHT_2_PENALTY=0.045851
PRIMER_LEFT_2_SEQUENCE=CGATTTCAAGGAAATGAAGTCC
PRIMER_RIGHT_2_SEQUENCE=AGTGCGCTACAAAAACCTTCTC
PRIMER_LEFT_2=1166,22
PRIMER_RIGHT_2=1311,22
PRIMER_LEFT_2_TM=59.945
PRIMER_RIGHT_2_TM=59.954
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=146
PRIMER_PAIR_PENALTY_3=0.1008
PRIMER_LEFT_3_PENALTY=0.054900
PRIMER_RIGHT_3_PENALTY=0.045851
PRIMER_LEFT_3_SEQUENCE=CGATTTCAAGGAAATGAAGTCC
PRIMER_RIGHT_3_SEQUENCE=GTGCGCTACAAAAACCTTCTCT
PRIMER_LEFT_3=1166,22
PRIMER_RIGHT_3=1310,22
PRIMER_LEFT_3_TM=59.945
PRIMER_RIGHT_3_TM=59.954
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=0.1008
PRIMER_LEFT_4_PENALTY=0.054900
PRIMER_RIGHT_4_PENALTY=0.045851
PRIMER_LEFT_4_SEQUENCE=GTCCGATTTCAAGGAAATGAAG
PRIMER_RIGHT_4_SEQUENCE=AGTGCGCTACAAAAACCTTCTC
PRIMER_LEFT_4=1163,22
PRIMER_RIGHT_4=1311,22
PRIMER_LEFT_4_TM=59.945
PRIMER_RIGHT_4_TM=59.954
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_06254 Ps_contig_mira-and-tgicl-ass_franssen_42535
SEQUENCE=GGTAGTTTGTCTGTTTTCTATGGTTCTACTTGGCTACTATTCCGATCACCCGGTAACCCGGGTAACGTTTAAACCCCGGTACCGAAAGAAAGTTTCGGTAGTCCGCTGTGATCTTAAACCAAGGGGAAAAGTTTAGGGTAGTGANCAGTGGGTTTCTGGGTTGGACCAATAATTGGAGCAACCGTGGCTGCAATTTACCCACCAATACATTCTAAGAGGATCAGCAATTAAAGCTCTTGGATCTCTATTCAGGAGCAACGCTTAATTGATTAATTAATTAAGCTAGCTAAATTGCTTAATTTACCAAGGAAGATATGGTGTTAATTTCCACACAAGAATAATGTTGATGAGTGAAAGTTAAGTGGTTCCTTTTGGGTTTTTTTCTTCCTTCTTTTTCGTTTTCAGTTTATAGCTGTAGTGGGATTTGGAAGAAAACAAAGAAACAAAAGAAGGGACCATAGAAATTGTAAGATATCCATTGTTTGTTGTTTCGAAGGGTTGTTAATTTTGTTACTTCCC
TARGET=269,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5234
PRIMER_LEFT_PENALTY=0.228464
PRIMER_RIGHT_PENALTY=0.294975
PRIMER_LEFT_SEQUENCE=GGATCTCTATTCAGGAGCAACG
PRIMER_RIGHT_SEQUENCE=ACAACCCTTCGAAACAACAAAC
PRIMER_LEFT=238,22
PRIMER_RIGHT=501,22
PRIMER_LEFT_TM=60.228
PRIMER_RIGHT_TM=60.295
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=264
PRIMER_PAIR_PENALTY_1=0.6633
PRIMER_LEFT_1_PENALTY=0.368304
PRIMER_RIGHT_1_PENALTY=0.294975
PRIMER_LEFT_1_SEQUENCE=GATCTCTATTCAGGAGCAACGC
PRIMER_RIGHT_1_SEQUENCE=ACAACCCTTCGAAACAACAAAC
PRIMER_LEFT_1=239,22
PRIMER_RIGHT_1=501,22
PRIMER_LEFT_1_TM=60.368
PRIMER_RIGHT_1_TM=60.295
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.9000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.7112
PRIMER_LEFT_2_PENALTY=0.228464
PRIMER_RIGHT_2_PENALTY=0.482784
PRIMER_LEFT_2_SEQUENCE=GGATCTCTATTCAGGAGCAACG
PRIMER_RIGHT_2_SEQUENCE=TTCTTCCAAATCCCACTACAGC
PRIMER_LEFT_2=238,22
PRIMER_RIGHT_2=432,22
PRIMER_LEFT_2_TM=60.228
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=0.7248
PRIMER_LEFT_3_PENALTY=0.242059
PRIMER_RIGHT_3_PENALTY=0.482784
PRIMER_LEFT_3_SEQUENCE=CTGCAATTTACCCACCAATACA
PRIMER_RIGHT_3_SEQUENCE=TTCTTCCAAATCCCACTACAGC
PRIMER_LEFT_3=187,22
PRIMER_RIGHT_3=432,22
PRIMER_LEFT_3_TM=59.758
PRIMER_RIGHT_3_TM=60.483
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=246
PRIMER_PAIR_PENALTY_4=0.7640
PRIMER_LEFT_4_PENALTY=0.469035
PRIMER_RIGHT_4_PENALTY=0.294975
PRIMER_LEFT_4_SEQUENCE=ATCTCTATTCAGGAGCAACGCT
PRIMER_RIGHT_4_SEQUENCE=ACAACCCTTCGAAACAACAAAC
PRIMER_LEFT_4=240,22
PRIMER_RIGHT_4=501,22
PRIMER_LEFT_4_TM=59.531
PRIMER_RIGHT_4_TM=60.295
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_06255 Ps_contig_mira-and-tgicl-ass_franssen_42547
SEQUENCE=GTTTTCTAATCAGATGTTATGTNTGTCTAGCAATAGGAAAGCATGAAACATTAAAATTTATAATTTAAGTTAAAAAAAAAGACATCCATCATCTTGTATTAGTGAACCTATGCATACACACATGATAATGATAATGATAATAATTCACATTGAATTGGGAAGAACATTACTATCACAAAAACATATAAATATAAGAGTGTTTATTTTCCGGGAACAAAGTTGGTGGCATATGACCATGCATTGTTGTTGACTGGGTCTGCAAGATGATCAGCAAGGTTCTCCAAAGGACCCTTTCCAGTTACAATAGCTTGAACGAAGAATCCAAACATTGAGAACATGGCTAATCTACCGTTCTTGAGTTCCTTCACCTTCAATTCTGCGAATGCTTCTGGATCATCAGCTAAGCCCAATGGATCAAAGCTTCCACCTGGGTAAAGTGGATCAACCACCTCACCGAGAGGCCCACCGGCAATACGGTAACCTTCGACAGCTCCCATCAAGATAACCTGAGTGGCCCATATGGCAAGGATGCTTTGAGCATGGACCAAGCTTGGGTTGCCCAAGTAATCAAGTCCACCCTCACTAAAGATTTGAGATCCTGCCTTGAACCACACAGCTTCGCCGAATTTAACACCGTTGCGAGACAAAAGCTCTGGGAAGACACATCCCAAAGCACCCAACATAGCCCATCTGGAGTGGATGACTTCAAGCTCACGGTTCTTGGAGAATGTCTCTGGGTCAGCAGAGAGTCCGGCAGTGTCCCAACCGTAGTCACCGGGGAACTCTCCAGTCAAGTAGGATGGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGCTTGATGGGTTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGTGTATTATATTGCAATGAAATTGAAAAGATTGTTATGCTTTTGATAATTGTATCCTTATCAATGGTCATTTTGTAAGATGAATATGAGTCTGTATTTATAATGCAGATGCTCTTATCGTGTGTTATCTAAAGCTATGGCTAGTTGGAGATTTTCTTTGCAATTCTAGAATCCGTTCATGGATCAAAATCTTCATATCATTTGTATCCAATGCTGCTCTTCCATGTGTGACTGTGGGGATTCTTATGGATATATGACTCAACATAATATTATTT
TARGET=988,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6016
PRIMER_LEFT_PENALTY=0.301926
PRIMER_RIGHT_PENALTY=0.299723
PRIMER_LEFT_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_SEQUENCE=CACACGATAAGAGCATCTGCAT
PRIMER_LEFT=877,22
PRIMER_RIGHT=1133,22
PRIMER_LEFT_TM=60.302
PRIMER_RIGHT_TM=60.300
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=0.6016
PRIMER_LEFT_1_PENALTY=0.301926
PRIMER_RIGHT_1_PENALTY=0.299723
PRIMER_LEFT_1_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_1_SEQUENCE=CACACGATAAGAGCATCTGCAT
PRIMER_LEFT_1=878,22
PRIMER_RIGHT_1=1133,22
PRIMER_LEFT_1_TM=60.302
PRIMER_RIGHT_1_TM=60.300
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=256
PRIMER_PAIR_PENALTY_2=0.9010
PRIMER_LEFT_2_PENALTY=0.753664
PRIMER_RIGHT_2_PENALTY=0.147295
PRIMER_LEFT_2_SEQUENCE=AGGTTGGAGAAGAGAGAGCCAT
PRIMER_RIGHT_2_SEQUENCE=CATAAGAATCCCCACAGTCACA
PRIMER_LEFT_2=964,22
PRIMER_RIGHT_2=1257,22
PRIMER_LEFT_2_TM=60.754
PRIMER_RIGHT_2_TM=59.853
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=9.6000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=294
PRIMER_PAIR_PENALTY_3=0.9604
PRIMER_LEFT_3_PENALTY=0.660713
PRIMER_RIGHT_3_PENALTY=0.299723
PRIMER_LEFT_3_SEQUENCE=CTTGATGGGTTCAGCTTGAGTT
PRIMER_RIGHT_3_SEQUENCE=CACACGATAAGAGCATCTGCAT
PRIMER_LEFT_3=930,22
PRIMER_RIGHT_3=1133,22
PRIMER_LEFT_3_TM=60.661
PRIMER_RIGHT_3_TM=60.300
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=204
PRIMER_PAIR_PENALTY_4=0.9604
PRIMER_LEFT_4_PENALTY=0.660713
PRIMER_RIGHT_4_PENALTY=0.299723
PRIMER_LEFT_4_SEQUENCE=TTCTTGGTGGTAGCAGACTTCC
PRIMER_RIGHT_4_SEQUENCE=CACACGATAAGAGCATCTGCAT
PRIMER_LEFT_4=876,22
PRIMER_RIGHT_4=1133,22
PRIMER_LEFT_4_TM=60.661
PRIMER_RIGHT_4_TM=60.300
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=258
=
PRIMER_SEQUENCE_ID=SSR_06256 Ps_contig_mira-and-tgicl-ass_franssen_42548
SEQUENCE=CGGGGACCGATTGATAAGGATACAATTATCTAAAAGCATAACAATCTTTTCAATTTCTATTTGCAATATAATACACGATGGCCGCATCATCATCATCATCCATGGCTCTCTCTTCTCCAACCTTGGCTGGCAAGCAACTCAAGCTGAACCCATCAAGCCAAGAATTGGGAGCTGCAAGGTTCACCATGAGGAAGTCTGCTACCACCAAGAAAGTAGCTTCCTCTGGAAGCCCATGGTACGGACCAGACCGTGTTAAGTACTTAGGCCCATTCTACCGGTAGAGTCTCCATCCTACTTGACTGGAGAGTTCCCCGGTGACTACGGTTGG
TARGET=85,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3425
PRIMER_LEFT_PENALTY=0.040562
PRIMER_RIGHT_PENALTY=0.301926
PRIMER_LEFT_SEQUENCE=GGGACCGATTGATAAGGATACA
PRIMER_RIGHT_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_LEFT=2,22
PRIMER_RIGHT=209,22
PRIMER_LEFT_TM=60.041
PRIMER_RIGHT_TM=60.302
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=208
PRIMER_PAIR_PENALTY_1=0.3425
PRIMER_LEFT_1_PENALTY=0.040562
PRIMER_RIGHT_1_PENALTY=0.301926
PRIMER_LEFT_1_SEQUENCE=GGGACCGATTGATAAGGATACA
PRIMER_RIGHT_1_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_LEFT_1=2,22
PRIMER_RIGHT_1=208,22
PRIMER_LEFT_1_TM=60.041
PRIMER_RIGHT_1_TM=60.302
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=207
PRIMER_PAIR_PENALTY_2=0.6296
PRIMER_LEFT_2_PENALTY=0.040562
PRIMER_RIGHT_2_PENALTY=0.589035
PRIMER_LEFT_2_SEQUENCE=GGGACCGATTGATAAGGATACA
PRIMER_RIGHT_2_SEQUENCE=GAGGAAGCTACTTTCTTGGTGG
PRIMER_LEFT_2=2,22
PRIMER_RIGHT_2=222,22
PRIMER_LEFT_2_TM=60.041
PRIMER_RIGHT_2_TM=59.411
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=5.6000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=221
PRIMER_PAIR_PENALTY_3=0.7013
PRIMER_LEFT_3_PENALTY=0.040562
PRIMER_RIGHT_3_PENALTY=0.660713
PRIMER_LEFT_3_SEQUENCE=GGGACCGATTGATAAGGATACA
PRIMER_RIGHT_3_SEQUENCE=TTCTTGGTGGTAGCAGACTTCC
PRIMER_LEFT_3=2,22
PRIMER_RIGHT_3=210,22
PRIMER_LEFT_3_TM=60.041
PRIMER_RIGHT_3_TM=60.661
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=209
PRIMER_PAIR_PENALTY_4=0.7013
PRIMER_LEFT_4_PENALTY=0.040562
PRIMER_RIGHT_4_PENALTY=0.660713
PRIMER_LEFT_4_SEQUENCE=GGGACCGATTGATAAGGATACA
PRIMER_RIGHT_4_SEQUENCE=CTTGATGGGTTCAGCTTGAGTT
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=156,22
PRIMER_LEFT_4_TM=60.041
PRIMER_RIGHT_4_TM=60.661
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_06257 Ps_contig_mira-and-tgicl-ass_franssen_42604
SEQUENCE=TTAAACAAAACAACCCTGCATGACATCCTTAAATTGCTAAGGGAAATGCTACAGACGAAGTTTATTATGTCTCTATGTGATAATGATACAGAATTTACAATCTTATTCAGTAATGTGGTTGGTATATGGAAAATAGAAGAAATTGAGTCTCAATTCCAAACTTATTTTACAGATTAACATCAAAGACTTATCATTATATCACTGCATTTTACGTTTAAGATGAATAACTACAAAGATAACGTGTAAGATGGAAGTCATCACCAAGGCATCCTTTTGGGCATCACGTCCCAGTTTGCCATTTAGGCCACAACAGGATAGGAAGAACATGTAGCAACACCGCACATATTCTTCCCCATTTCCATTTTGAAATAGCCATGGTCACCCCAATCACCTCCCCACGAATTTTTAATGAGCCAATATGGTATGCCGTCTTCAATCCCATATCCAACAGCAAGAACAGCATGATTCACATCCATGGGCGTGCTGCCACAAGCTGTACTAGTGTAAACTCCACTCTTGTATAATCTGAAGTCATGCACCACCTCAAATGCCACACTAACAGGCCGAGCAAAAGCAATTGCGTGTTTCAATTCATCCTCAGCACCCAAGGTGATGTTGACAGAGCCAAGGACTTTAACAGCAACGTGTTCAGATCTAAATTTGCAGAGACCATTACTTCCAGTGTAGGGATATGCTTCTTCTGTCTCAAGGCCACCATTGTATTTTATGTATTCAAAGGCTTGTGACGGTAACCCGCCGCTGCAGCCAAAGTTATTGAAAGCACCAGCACAATCCACTAACTGCTGCTCAGAAAGAGAGATATTCTTTCCGAATGCCTGCGCGTAAGCTGATTCCAATGCTCCAGTTGTGCTGAATGTCCAGCATGATCCGCAGCTGCCTTGATCTTTAACTCCACTGACTATACCTTCCCTCCTCCAGTCTTTCTCGTCAGGAAGATTAGCATCGGTAATCTTATGGTTACCCTTAAGAGTAGCAGAGCAATTTTGAGCAGCACCAAGTCTATGACTTCTGAACTCCTCCCAAGTCCAATCAGCAAAATGATTAACACCGAGCTTGTAACTGAGTCGTCTCTTATTACTGGACCTGATAAGTTCAATATTCTCCGAAAAGATCTTAAACCGAAGCTTCATCTCATCAACGCTATCGTATCGCTTACCGTATCTGTTAGCGAACCGGGCGAAAGAGACGGCGTGACGACTTTCTCCGATCACCTGAAGTAACTGCTCCTCCACGTCGGAGACCATTCGAATAGGATTGGAGTCATGGAAGCTGAATCCGGCGGCGGCGGAGGCGACGCAGAACAAAACGATAAGCAACGACCACTGTGCCATATCACTCTCCCAAAACTCTGGTACTAAAAGTTTCTACGTTTTACTCTTCCCCCGAGTACTCTGCGTTGACTACCCA
TARGET=1298,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2646
PRIMER_LEFT_PENALTY=0.031769
PRIMER_RIGHT_PENALTY=0.232784
PRIMER_LEFT_SEQUENCE=ATTGGAGTCATGGAAGCTGAAT
PRIMER_RIGHT_SEQUENCE=CAGAGTACTCGGGGGAAGAGTA
PRIMER_LEFT=1274,22
PRIMER_RIGHT=1414,22
PRIMER_LEFT_TM=59.968
PRIMER_RIGHT_TM=59.767
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=54.545
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=5.4000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=141
PRIMER_PAIR_PENALTY_1=0.3085
PRIMER_LEFT_1_PENALTY=0.246726
PRIMER_RIGHT_1_PENALTY=0.061818
PRIMER_LEFT_1_SEQUENCE=GACTTTCTCCGATCACCTGAAG
PRIMER_RIGHT_1_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_LEFT_1=1216,22
PRIMER_RIGHT_1=1344,22
PRIMER_LEFT_1_TM=60.247
PRIMER_RIGHT_1_TM=60.062
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.3637
PRIMER_LEFT_2_PENALTY=0.301926
PRIMER_RIGHT_2_PENALTY=0.061818
PRIMER_LEFT_2_SEQUENCE=CCTGAAGTAACTGCTCCTCCAC
PRIMER_RIGHT_2_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_LEFT_2=1231,22
PRIMER_RIGHT_2=1344,22
PRIMER_LEFT_2_TM=60.302
PRIMER_RIGHT_2_TM=60.062
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=114
PRIMER_PAIR_PENALTY_3=0.3637
PRIMER_LEFT_3_PENALTY=0.301926
PRIMER_RIGHT_3_PENALTY=0.061818
PRIMER_LEFT_3_SEQUENCE=CACCTGAAGTAACTGCTCCTCC
PRIMER_RIGHT_3_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_LEFT_3=1229,22
PRIMER_RIGHT_3=1344,22
PRIMER_LEFT_3_TM=60.302
PRIMER_RIGHT_3_TM=60.062
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=116
PRIMER_PAIR_PENALTY_4=0.3717
PRIMER_LEFT_4_PENALTY=0.309858
PRIMER_RIGHT_4_PENALTY=0.061818
PRIMER_LEFT_4_SEQUENCE=GAAGTAACTGCTCCTCCACGTC
PRIMER_RIGHT_4_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_LEFT_4=1234,22
PRIMER_RIGHT_4=1344,22
PRIMER_LEFT_4_TM=60.310
PRIMER_RIGHT_4_TM=60.062
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.8000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=111
=
PRIMER_SEQUENCE_ID=SSR_06258 Ps_contig_mira-and-tgicl-ass_franssen_42605
SEQUENCE=CTGGATAAACACCATCCTTAGGATGCAAATATTGAACTTCACCACTAGGGAACACCCTATAAAACTGATACTTAATCTTGTACTTTGACCTCAATCGAGTTCCTAAAGCCAAACACTGTTCTTTCCTAGCCAATTTGAGAAGATTAGGACCCTCTCTCATTATAGCTGCACCACCAGTTGGCATCTCAAAAATCTGTTCTTTAGGTGATTCCCATGTGATAACATAGAATTCTTCCACTTGGGCCTTACGCAATAATCCACCAGTGCTTCCACCGAAAATTGGAGAAGGTGTGTTTGGGTCTAGCTCAGGTGGGGTGAATCCAACTGGTGCTTCTTTTGTTGCTACATCGGTTGCTGCATCGGTTTTGTCTTCTGTTGCAGCAATTTTTGTTGTTCTTGGTGTGAACTTGGTTGGTTTGAGAGAGATGAAAGATAGAGTGGAGGGTTGTTTCCATGTTTTGGGAGTTTGAGAGTGGTGGTGTGAAGAGAGAGGCTTGTGTTGCCATTGCCATGGTGAATGAATGAATGTTGTGAGATCAATAGTGTGAAGAATGAATGAATCCCCCTANTGCGTT
TARGET=515,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4518
PRIMER_LEFT_PENALTY=0.039879
PRIMER_RIGHT_PENALTY=0.411917
PRIMER_LEFT_SEQUENCE=ATCCAACTGGTGCTTCTTTTGT
PRIMER_RIGHT_SEQUENCE=CANTAGGGGGATTCATTCATTC
PRIMER_LEFT=318,22
PRIMER_RIGHT=570,22
PRIMER_LEFT_TM=60.040
PRIMER_RIGHT_TM=59.588
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=42.857
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.75
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=253
PRIMER_PAIR_PENALTY_1=0.5423
PRIMER_LEFT_1_PENALTY=0.130358
PRIMER_RIGHT_1_PENALTY=0.411917
PRIMER_LEFT_1_SEQUENCE=GTTGTTTCCATGTTTTGGGAGT
PRIMER_RIGHT_1_SEQUENCE=CANTAGGGGGATTCATTCATTC
PRIMER_LEFT_1=444,22
PRIMER_RIGHT_1=570,22
PRIMER_LEFT_1_TM=60.130
PRIMER_RIGHT_1_TM=59.588
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=42.857
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=127
PRIMER_PAIR_PENALTY_2=0.5479
PRIMER_LEFT_2_PENALTY=0.136017
PRIMER_RIGHT_2_PENALTY=0.411917
PRIMER_LEFT_2_SEQUENCE=ATAGAGTGGAGGGTTGTTTCCA
PRIMER_RIGHT_2_SEQUENCE=CANTAGGGGGATTCATTCATTC
PRIMER_LEFT_2=432,22
PRIMER_RIGHT_2=570,22
PRIMER_LEFT_2_TM=59.864
PRIMER_RIGHT_2_TM=59.588
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.5000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=0.5479
PRIMER_LEFT_3_PENALTY=0.136017
PRIMER_RIGHT_3_PENALTY=0.411917
PRIMER_LEFT_3_SEQUENCE=TAGAGTGGAGGGTTGTTTCCAT
PRIMER_RIGHT_3_SEQUENCE=CANTAGGGGGATTCATTCATTC
PRIMER_LEFT_3=433,22
PRIMER_RIGHT_3=570,22
PRIMER_LEFT_3_TM=59.864
PRIMER_RIGHT_3_TM=59.588
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=42.857
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=138
PRIMER_PAIR_PENALTY_4=0.5775
PRIMER_LEFT_4_PENALTY=0.165541
PRIMER_RIGHT_4_PENALTY=0.411917
PRIMER_LEFT_4_SEQUENCE=TCTTTTGTTGCTACATCGGTTG
PRIMER_RIGHT_4_SEQUENCE=CANTAGGGGGATTCATTCATTC
PRIMER_LEFT_4=332,22
PRIMER_RIGHT_4=570,22
PRIMER_LEFT_4_TM=60.166
PRIMER_RIGHT_4_TM=59.588
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=239
=
PRIMER_SEQUENCE_ID=SSR_06259 Ps_contig_mira-and-tgicl-ass_franssen_42605
SEQUENCE=CTGGATAAACACCATCCTTAGGATGCAAATATTGAACTTCACCACTAGGGAACACCCTATAAAACTGATACTTAATCTTGTACTTTGACCTCAATCGAGTTCCTAAAGCCAAACACTGTTCTTTCCTAGCCAATTTGAGAAGATTAGGACCCTCTCTCATTATAGCTGCACCACCAGTTGGCATCTCAAAAATCTGTTCTTTAGGTGATTCCCATGTGATAACATAGAATTCTTCCACTTGGGCCTTACGCAATAATCCACCAGTGCTTCCACCGAAAATTGGAGAAGGTGTGTTTGGGTCTAGCTCAGGTGGGGTGAATCCAACTGGTGCTTCTTTTGTTGCTACATCGGTTGCTGCATCGGTTTTGTCTTCTGTTGCAGCAATTTTTGTTGTTCTTGGTGTGAACTTGGTTGGTTTGAGAGAGATGAAAGATAGAGTGGAGGGTTGTTTCCATGTTTTGGGAGTTTGAGAGTGGTGGTGTGAAGAGAGAGGCTTGTGTTGCCATTGCCATGGTGAATGAATGAATGTTGTGAGATCAATAGTGTGAAGAATGAATGAATCCCCCTANTGCGTT
TARGET=550,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06260 Ps_contig_mira-and-tgicl-ass_franssen_42630
SEQUENCE=TTGCGTGTCTCAGAAGCAGTGGTATCAACGCAGAGTTGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTTGTGTACACTTCTACCAGAAAGATATGGTTGTTGTTTACTTGTAACATTTTCATCATCTTTGCTTGAATTGTAACTCAAAAGTCTTAGCTTGTTTTGTTAGCTTGAGTTCTTTGTAAATCACTTCAGTTTAGTGTTTCAACAATAATAATTTATCATGATTCCTTTATTTTCTAGCTAAAACTTGTTTGCTACTTGATTTGRTTCTTCTCAACAATTTTTATGTATTATTTTTGTTTNCTTGATT
TARGET=194,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06261 Ps_contig_mira-and-tgicl-ass_franssen_42631
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGGTAGTAAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTTACTGTGTACACTTCTAA
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06262 Ps_contig_mira-and-tgicl-ass_franssen_42639
SEQUENCE=ACCAAGAAATTGAATATAGGTTATTATTATTATTGCGGCAGGAAGATAAAACTTACATACATAGAAAAAAAAAACATCATGAATTAAAGACTCTCTATATACACTACACATAGATACATATTACCCTACCCTTTGGACTTCCTTTCAAACTTAAACATGCTATAACCAAACCTGAACTGCAGCACAAAACTTCAATTCAACTCAACCCATACACACAAATATACCAAACAAAATTTATTACATCTAAGGCCAAAAGGTAACATCCTCTGCTTCTGGAGACTAGAAAATGCTGTGCCTCATTTCTCACACATCGAAAGGAGAAGAAGAGCTTGATCTGTCTTGGTTTTTCTTCTCGAACGCATCCGATGCCGACCTTCTTCGAAACCGAAATGATTCTCTAGTTCCGGAAAACGGAGACACCGGAGTAACTGAACCGGCGGGAGAAGCCGGAGGCGATCCACTTTGGTAACCTGGCGGTTTCACGATCATGATACTACGCGTTACTCTCGTCGCTGGCTCTGACGGTTCCTCACCGTACGATCTCACGCTTCCAGCCTCCAATTCCTTGCCGGAGCTAGATCGGGCAGCGAACGTGTTATGCTTACGAAGTTTTCCGAGGCCGTTCTCCGGCCGAGGACCGGCGACTGTATCGTCCCACAACTGGTCGAGGAGGCCCATATATGAATAAGGCGTATTTTAGCAGATGCGGCGTATCTGCGATCCC
TARGET=21,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06263 Ps_contig_mira-and-tgicl-ass_franssen_42649
SEQUENCE=GTCGGGGGGTTACCTTATCCAAGCGNGTTTCCATCCCAACGGCATATTACGATGCTATGCTATCATCCACTCCTCAATCCAACTTCCCTGTCACTACAACCACCTTCCGTCAGTTAAACTAAACTCTAACAATGGCTTCCACGTTTCATCTCTCAACTCCTTTCTCTCAAATCCACCCTCTCAAACCCAAACCCAAACCCTCCCTCTTCCACCTCTCCAAAACCCATCTCTCCCACAATCTCAAACCCATCCACCCTCGAAAATTCACCATTAACTCCACCGACATTTCAAAAGAAGAAGAACAACAGCAACAACAAGCTTCACCCGATTCCGAAAAGCTTCGCCTGGAAGAGAAATTCGCTGTCTTGAACACCGGAATCTACGAATGTCGTTCATGTGGGTATAAATACGACGAAGGTGTTGGTGACCCTTCGTATCCCATTCCTCCTGGTTATCAGTTCGAGAAGCTTCCAGATGATTGGAGATGTCCGACTTGTGGTGCGGCGCAGAGTTTCTTTGAGAGCAAAAGTGTTGAGATTGCTGGGTTTGCTCAGAATCAACAGTATGGACTTGGTGGAAATTCTCTTACTTCTGGTCAGAAAACTGTGCTTATTTATGGTACTTTGTTGTTTTTCTTTGGGCTTTTTCTTAGTGGCTACTTCATACAATAGTTATTATTATCATTCTTTTTTGTTTCTGAATTTATGTTGGATTTGATGCTTTGTTGGTCACAAGTGTATGTAATGTTGTCAATACTCAATAGTAAGAGCCAAGTATGAGGGTTATCAGTCAATCATCTTACACATTACAAACCATTTCCTTTGTAGAAATATGACTATGGAAGGAAATTGGTATTGCAATACTGCAATTCAATCATTCTCTTAGAAAGTGAAACTTACTCTCTCTCTACTATATGYTTGCAATTCAATCATTCTTTGACTATAAGGTTGATTATTTGTCTACAAGC
TARGET=899,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06264 Ps_contig_mira-and-tgicl-ass_franssen_42659
SEQUENCE=TGTGATAAGCTTAAGCACTGGTCTGTTCTAATTGTTTTTAGGAAGGTTTGTGTTCTTCAACTTCCAAAGAGAGAATGTTGCAAAACAAGGTTTACCCTGAACAGAATGGTGTGACACACTTTGAAGCTGGTGACTCACGTGCTAAGGAATATGTTAGCTTGCTTAAGTCAAATGATCCTGTCGGGTTTAACCTTGTTGATGTTTTGGCTTGGGGTTCTATAGGTCATATTGTTGCTTATTATATCTTGGCCACTTCTAGCAATGGTTATGATCCTAGTTTCTTTGGTTAATTAAGATTACTATGTTGTTCGTTTATGTTGTTTTAATTTGTCTTTTCTTGTCCCTTTTATGTTTATTAAGATGATGATGATGATGATGATGATGTATAGTTTATTTGTATGAGATATTCAACAAGTTAAGTTCCTTGGATTAAAGCATTTTGTATTTAAGG
TARGET=360,24
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06265 Ps_contig_mira-and-tgicl-ass_franssen_42677
SEQUENCE=CAACGCAGAGTACNCGGGGGTGTCTGCGCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCGGACCTTTGCCGGAGAATGGTCTCGGCAAGCTCCGAAAACACCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGAATCAGAGGGTGGAAACGTGAGATCCTACGGTGGGGATTCGTTAGAGGGTGCAGTGAAAGTGACACGTAGTATCATGATAGTGAAACCAGCGGGGTACCAGAGTCCA
TARGET=124,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3621
PRIMER_LEFT_PENALTY=0.275745
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT=42,22
PRIMER_RIGHT=219,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.6994
PRIMER_LEFT_1_PENALTY=0.275745
PRIMER_RIGHT_1_PENALTY=0.423703
PRIMER_LEFT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_1_SEQUENCE=ATTCCTTGTCGGAGATAGATCG
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=159,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=59.576
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=0.7268
PRIMER_LEFT_2_PENALTY=0.640429
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=GTGTCTGCGCTTTCCATATTCT
PRIMER_RIGHT_2_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT_2=19,22
PRIMER_RIGHT_2=219,22
PRIMER_LEFT_2_TM=60.640
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.8750
PRIMER_LEFT_3_PENALTY=0.275745
PRIMER_RIGHT_3_PENALTY=0.599276
PRIMER_LEFT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_3_SEQUENCE=GTCACTTTCACTGCACCCTCTA
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=220,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.401
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=179
PRIMER_PAIR_PENALTY_4=0.9862
PRIMER_LEFT_4_PENALTY=0.275745
PRIMER_RIGHT_4_PENALTY=0.710449
PRIMER_LEFT_4_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_4_SEQUENCE=ACTTTCACTGCACCCTCTAACG
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=217,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=60.710
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=176
=
PRIMER_SEQUENCE_ID=SSR_06266 Ps_contig_mira-and-tgicl-ass_franssen_42680
SEQUENCE=TGACTTCAAGCTCACGGTTCTTGGAGAATTGTCTCTGGGTCAGCAGAGAGTCCGGCAGTGTCCCAACCCGTAGTCACCGGGGAACTCTCCAGTCAAGTAGGATGGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTACAGCTCCCAATTCTTGGCTTGAGTGGGGTCAGTCTTGAGTTGCTYGCCACGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGTGTATTATATTGCAATGAAATTGAAAAGATTGTTATGCTTTTGATAATTGTATCCTTATCAATAGGTACCCCC
TARGET=293,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06267 Ps_contig_mira-and-tgicl-ass_franssen_42694
SEQUENCE=CAAAGCATCCTTGCCATATGGGGCCTACTTCAAGGTTATCTTGATGGGAGCTGTTGAAGGTTACCGTATTGCCGGTGGCCCTCTTGGTGAGGTTGTTGACCCACTTTACCCAGGTGGTAGCTTTGACCCATTGGGCTTAGCTGACGACCCAGAAGCATTCGCAGAATTGAAGGTGAAGGAACTGAAGAACGGTAGATTAGCTATGTTCTCAATGTTTGGATTCTTTGTTCAAGCTATTGTGACCGGAAAGGGACCTTTGGAGAATCTTGCTGATCATCTTTCCGACCCAGTTAACAACAATGCATGGTCATATGCCACCAACTTTGTTCCCGGAAAGTGAGTCGTCGTATTGTGTTTTTGTGAAGAGGTCATTTTAATGTATTTCTCATTGTGAATTTATTATCATGTTTGTATGCAACTGAATGTTAGTTTTTTTTTTCAGTGTTTATTTGTTCTCTATTCCAATTCCCAGCACTAGCTTTCATATAATAAATATGATGTATGCCACCAAAATTGAATTAATTTTAATTTTGTCGCATAAGTTTAGGGNAATAAATATATAAATATAAATAATAAATAAATATAATAATACTTCTATGTTCTAATT
TARGET=572,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06268 Ps_contig_mira-and-tgicl-ass_franssen_42708
SEQUENCE=TCACCAGGATATTATGATGGAAGATACTGGACAATGTGGAAGCTTCCTATGTTTGGTACCACTGATGCTTCTCAAGTCTTGAAGGAGCTTGATGAAGTTGTTGCCGCTTACCCCCAAGCTTTCGTCCGTATCATCGGTTTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCGCACACACCAGAATCCTACTAAGTTTGAGTATTATGGCATTGGAAAAACTGTTTCTCTTGTACCATTTGTTCTGCTTGTAATTTACTGTGGTTTTTTTATTCGGACTGTAAAATGCAAATGGATGGATAAGAGTTAATCAATGATATGGTCCTTTTGTTCATTCTCAACTTAGTGTTATCTTTTCTTCTTCTTCAYTNGTTTTGTGTTGAATTTGAAATTTGAGGTAAACAAAGGAAGTAACTGT
TARGET=362,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06269 Ps_contig_mira-and-tgicl-ass_franssen_42714
SEQUENCE=ACCTAGAAAGAAAGCAAAGCAATAACAAGGTTTTGCATATTATACATATACGAGCATTAACTAGTTAGGTTAGATACAGTTTTACACGTTACAAGTTCCTATATATATTTAAACAAACAAAACATTATTATTAGCAACTCTGGAGACTAAAAAAAACAACATGCTAACTATTTGCAAGAACTACACAACCTCTATATACATCAAACACACACAAACACACACAAACACACAAAGCAAAACTGCGTTGGATACAGCTTGAGCCAAAACGAAAACGAGCACACATCGATCCAATGATCTTCACTATCACTAACTAGCTTCACTTATCACACATCGAAAGGAGAAGAAGAGCTTGGTCTGTTTTGGCCTGTCTTGACCTCGCTCTCGTACGCATCTGATATTGACCTTCTTCGAAAACGAAACGGCTCTCTAGCTCCGGAAAACGGAGATAGCGGCGGAGTAGAACCAGCGGGGGAAGCCGGAGCTGATCCACTTGGACTCTGGTACCCAGCTGGTTTCACTATCATGATACTACGTGTCACTTTCACTGCACCCTCTAACGAATCCCCACCGTAGGATCTCACGTTTCCACCCTCTGATTCCTTGTCGGAGATAGATCGGGAAAGGAAGGAAGGGTGTTTTCGGAGCTTGCCGAGACCATTCTCCGGCAAAGGTCCGGCGACGGTGTCATCCCACAAGTGATCGAGAAGGCTCATGAGTAA
TARGET=621,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1087
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.022341
PRIMER_LEFT_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_RIGHT_SEQUENCE=TACTCATGAGCCTTCTCGATCA
PRIMER_LEFT=535,22
PRIMER_RIGHT=717,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=59.978
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=8.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=183
PRIMER_PAIR_PENALTY_1=0.1305
PRIMER_LEFT_1_PENALTY=0.108206
PRIMER_RIGHT_1_PENALTY=0.022341
PRIMER_LEFT_1_SEQUENCE=ACCCAGCTGGTTTCACTATCAT
PRIMER_RIGHT_1_SEQUENCE=TACTCATGAGCCTTCTCGATCA
PRIMER_LEFT_1=502,22
PRIMER_RIGHT_1=717,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=59.978
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=216
PRIMER_PAIR_PENALTY_2=0.3621
PRIMER_LEFT_2_PENALTY=0.086339
PRIMER_RIGHT_2_PENALTY=0.275745
PRIMER_LEFT_2_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_RIGHT_2_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_2=535,22
PRIMER_RIGHT_2=712,22
PRIMER_LEFT_2_TM=59.914
PRIMER_RIGHT_2_TM=60.276
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.0000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=178
PRIMER_PAIR_PENALTY_3=0.3840
PRIMER_LEFT_3_PENALTY=0.108206
PRIMER_RIGHT_3_PENALTY=0.275745
PRIMER_LEFT_3_SEQUENCE=ACCCAGCTGGTTTCACTATCAT
PRIMER_RIGHT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_3=502,22
PRIMER_RIGHT_3=712,22
PRIMER_LEFT_3_TM=59.892
PRIMER_RIGHT_3_TM=60.276
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.5000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=0.3905
PRIMER_LEFT_4_PENALTY=0.368190
PRIMER_RIGHT_4_PENALTY=0.022341
PRIMER_LEFT_4_SEQUENCE=TACCCAGCTGGTTTCACTATCA
PRIMER_RIGHT_4_SEQUENCE=TACTCATGAGCCTTCTCGATCA
PRIMER_LEFT_4=501,22
PRIMER_RIGHT_4=717,22
PRIMER_LEFT_4_TM=59.632
PRIMER_RIGHT_4_TM=59.978
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=217
=
PRIMER_SEQUENCE_ID=SSR_06270 Ps_contig_mira-and-tgicl-ass_franssen_42732
SEQUENCE=ACAGTAGAAAAACATGATAATATGATAGTATTTAAAGAAAGAACNGTAGAGAAACAAAATCATATACTCACGAATTTATTGCTCCACATTGATACAAGGAATTCAATTACAGATAGGGAATGAATTGAAAGAAGAAGAAGAAGAAATAATACAAGAAAATAGGTTGAATAAAATAACAACTATTATAAGCATAAGCATTACTTAGAAAAATAAATTACATATATAGATATAAATAAGGCTTAATTAGCCGCTTCCACCAAGAACACTCTTGAGTTTCTTGATAAGAACAGGATCAAGGAAAGTGGTCTTTGCAATCAAAGGAGTAGAGAAATTGCTTGCAAAAAGTGCAAAGTCAAGAATCTGAAGACCAGGACTAGCACTACTAAAAACAGGAAAAGCAACAGCTTTTCTCTTACCAGCAGTAGCTTGAAAATGCAACAAACCTTGTGGAAAAACCATGAGTTCACCTTTCTTCAAAGTTTTGATATAAACAGTGTTAGCTGAAGAAACAAATCCAGCAGTGATGTGACCTTGTGTCACAATAAGAAGCTCACTTGCACCCGGGTGACTGTGTAGAGGGATAACACCTGCAGGGCCAAGGTCTAACCTAGCTGCTGAGAGACCAAGTCCATTAAGGCCTGGAAATTGTGCGACAAATGCAGGCGTAACTGCGGCGTTGATTATGTTTGTTACGTTTGCTGGCTCTATTAAGCCTGCAAATGCAAAGTCTTCTACGGTTACAGTTGTTGCTGACTTGCAAGGGAAGCCTGAAGGGGTGTCTGAGGCTTTGAGATCTGCTACACAGAAGTCTTGGACAGAAGCATTGGAAATGGAGAGGAAAAGGGAGAGGAGGAAAATGATTTGAAACATGTTTGGTTGTTGGTCGATGAATTTGGTTTGAATGTNAATAACCCCGAA
TARGET=129,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1544
PRIMER_LEFT_PENALTY=0.130883
PRIMER_RIGHT_PENALTY=0.023556
PRIMER_LEFT_SEQUENCE=CTCACGAATTTATTGCTCCACA
PRIMER_RIGHT_SEQUENCE=CAAAGACCACTTTCCTTGATCC
PRIMER_LEFT=66,22
PRIMER_RIGHT=310,22
PRIMER_LEFT_TM=60.131
PRIMER_RIGHT_TM=59.976
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=245
PRIMER_PAIR_PENALTY_1=0.4943
PRIMER_LEFT_1_PENALTY=0.130883
PRIMER_RIGHT_1_PENALTY=0.363436
PRIMER_LEFT_1_SEQUENCE=CTCACGAATTTATTGCTCCACA
PRIMER_RIGHT_1_SEQUENCE=GATTGCAAAGACCACTTTCCTT
PRIMER_LEFT_1=66,22
PRIMER_RIGHT_1=315,22
PRIMER_LEFT_1_TM=60.131
PRIMER_RIGHT_1_TM=59.637
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=250
PRIMER_PAIR_PENALTY_2=0.5151
PRIMER_LEFT_2_PENALTY=0.130883
PRIMER_RIGHT_2_PENALTY=0.384229
PRIMER_LEFT_2_SEQUENCE=CTCACGAATTTATTGCTCCACA
PRIMER_RIGHT_2_SEQUENCE=CCACTTTCCTTGATCCTGTTCT
PRIMER_LEFT_2=66,22
PRIMER_RIGHT_2=304,22
PRIMER_LEFT_2_TM=60.131
PRIMER_RIGHT_2_TM=59.616
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.5151
PRIMER_LEFT_3_PENALTY=0.130883
PRIMER_RIGHT_3_PENALTY=0.384229
PRIMER_LEFT_3_SEQUENCE=CTCACGAATTTATTGCTCCACA
PRIMER_RIGHT_3_SEQUENCE=AAGACCACTTTCCTTGATCCTG
PRIMER_LEFT_3=66,22
PRIMER_RIGHT_3=308,22
PRIMER_LEFT_3_TM=60.131
PRIMER_RIGHT_3_TM=59.616
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=243
PRIMER_PAIR_PENALTY_4=0.6447
PRIMER_LEFT_4_PENALTY=0.130883
PRIMER_RIGHT_4_PENALTY=0.513831
PRIMER_LEFT_4_SEQUENCE=CTCACGAATTTATTGCTCCACA
PRIMER_RIGHT_4_SEQUENCE=CCTTTGATTGCAAAGACCACTT
PRIMER_LEFT_4=66,22
PRIMER_RIGHT_4=320,22
PRIMER_LEFT_4_TM=60.131
PRIMER_RIGHT_4_TM=60.514
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_06271 Ps_contig_mira-and-tgicl-ass_franssen_42744
SEQUENCE=GAGGAATCGGTTGAAGAAGTGAAGGAAGAGGCGGAAGCAGTAGTGGAGGAGCCAATAGCAACTACTGAAAATGAGTCAGCACCACCACCACCAGCAACTGCAGAGGATGTGATTGAGAACAAAACAGTTGAATCAGTGGAAGCTCCAGCAGCAGAGGAAGTTCCAGTTGAGAATCCTGAAGCTTAAACCAATGGAATGGGTGTGACTGGAACTGGTAAAAAACGGAAGTTTAAATTAATGGTTCTAGATGTTGGGATCGGGTCTACCATTTTGCATGTCATCAAGGTTGAAAAGCTTTTCTATTAATTATTTCTTTAGTTTCCTTTCCTATATTAAAGTTTATTATTGTTATTATGGTATGGCTTTTGGATATGGTTATTTATATGGCACTTGGCTCCACATCAGCATATATATACATGCTGAGTGGTGGTGGACATGTGAAACTCTATTCCATGAGTTGTGTATTGAAGTGATAAGGGGCTATAGTTCTTAAGAAGGTTTGTCATGGTGTGTTACTGTGTTTCTCTCTAGATTTGAATAAATAATATTTATTTAATATTNCTAAARAANAAT
TARGET=80,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06272 Ps_contig_mira-and-tgicl-ass_franssen_42754
SEQUENCE=AGGGGGTATGATGATGATGATGAGNAACGNNGATGTTGTCGGGGGAAGTGAAGGACTCCGATTGTTGATTCACACAACTTAACTTCTCTTCGTCTTCCAGAGACGTGAAAAACTCACCCATGGCAACCGTTCCTTTGGCCTCAGCTGTTCTAAGAAGGCTTGAGGGTAAGGTGGCTCTGATCACCGGTGGTGCTGGCGGCATTGGCGAAGCCACCGCAAGACTCTTCTCCAACCATGGAGCTAAAGTCGTAATAGCTGACCTCCAAGACGACAAAGGTCACTCAATTTGCCAGGAGTTACACAAATCATCTGCCTCTTATGTTCACTGTGACGCGACAAAGGAAGAAGACATAGAAACCGCCGTGAACACGGCGGTTTCCACTTACGGTAAACTGGACATCATGATAAACAACGCTGGCATATCCGGTGCGAATAAAACCAACATACTTGAAAACACGTTATCTGAATTTCAACAAGTGGTTAACGTTGAACTTGGTTGGTGTCTTTCTTGGAACAAAACATGCTGCAAGAGTAATGATCCCTGCTCGACGAGGTAGCATAATTAACACAGCTAGTATTGCTGGAAACATAGGTGGTACGATGAATCATGCTTACACAAGTTCAAAGCACGCCGTTGTGGGGCTGACGAGAAACACGGCGGTCGAGCTTGGACCGTTCGGTATTCGGGTGAATTGTGTGTCGCCATATATTGTTCTCACGCCGATGTCTAAGAAATACTTGGAGCTAGATGATGATGGGATTCTAGGGTTTTATTCTAACCTAAAGGGTACTAATCTTTTGCCAAAGG
TARGET=8,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06273 Ps_contig_mira-and-tgicl-ass_franssen_42801
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTCTTTTTTCTTTGTTGTACGTAACCTACCTTA
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06274 Ps_contig_mira-and-tgicl-ass_franssen_42842
SEQUENCE=TCAACGCAGAGTACGCGGGGACCATTGATAAGAATACAATTATCAAAAGCATAACAATCTTTTCAATTTCATTGCAATATACACACACGATGGCCGCATCATCATCATCATCCATGGCTCTCTCTTCTCCAACCTTGGCTGGCAAGCAACTCAAGCTGACCCCATCAAGCCAAGAATTGGGAGCTGCAAGGTTCACCATGAGGAAGTCTGCTACCACCAAGAAAGTAGCTTCCTCTGGAAGCCCATGGTACGGAC
TARGET=97,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7303
PRIMER_LEFT_PENALTY=0.428401
PRIMER_RIGHT_PENALTY=0.301926
PRIMER_LEFT_SEQUENCE=CGGGGACCATTGATAAGAATAC
PRIMER_RIGHT_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_LEFT=15,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=59.572
PRIMER_RIGHT_TM=60.302
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=207
PRIMER_PAIR_PENALTY_1=0.7303
PRIMER_LEFT_1_PENALTY=0.428401
PRIMER_RIGHT_1_PENALTY=0.301926
PRIMER_LEFT_1_SEQUENCE=CGGGGACCATTGATAAGAATAC
PRIMER_RIGHT_1_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_LEFT_1=15,22
PRIMER_RIGHT_1=220,22
PRIMER_LEFT_1_TM=59.572
PRIMER_RIGHT_1_TM=60.302
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=54.545
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=206
PRIMER_PAIR_PENALTY_2=0.9293
PRIMER_LEFT_2_PENALTY=0.627348
PRIMER_RIGHT_2_PENALTY=0.301926
PRIMER_LEFT_2_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_2_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_LEFT_2=71,22
PRIMER_RIGHT_2=221,22
PRIMER_LEFT_2_TM=59.373
PRIMER_RIGHT_2_TM=60.302
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=151
PRIMER_PAIR_PENALTY_3=0.9293
PRIMER_LEFT_3_PENALTY=0.627348
PRIMER_RIGHT_3_PENALTY=0.301926
PRIMER_LEFT_3_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_3_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_LEFT_3=71,22
PRIMER_RIGHT_3=220,22
PRIMER_LEFT_3_TM=59.373
PRIMER_RIGHT_3_TM=60.302
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=150
PRIMER_PAIR_PENALTY_4=1.0174
PRIMER_LEFT_4_PENALTY=0.428401
PRIMER_RIGHT_4_PENALTY=0.589035
PRIMER_LEFT_4_SEQUENCE=CGGGGACCATTGATAAGAATAC
PRIMER_RIGHT_4_SEQUENCE=GAGGAAGCTACTTTCTTGGTGG
PRIMER_LEFT_4=15,22
PRIMER_RIGHT_4=234,22
PRIMER_LEFT_4_TM=59.572
PRIMER_RIGHT_4_TM=59.411
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=220
=
PRIMER_SEQUENCE_ID=SSR_06275 Ps_contig_mira-and-tgicl-ass_franssen_42852
SEQUENCE=CATTCAAACAAACACTAAATACTAAGATAANAAGATAACACATAGTAAACATTACCAGTTTAATTATTATTTCATATTTAGTTCACAGGAATACAGTTCACAAGATACACATAAACATAATTAAAACACTTTATTTATTACTTTTATTACCCATAAACACACACATATACATATTATATGTATATGTGTACGTCACGGAAGCCCCATTTAATTTCCAATCCTGGCTCCAGTGTTGATCTTGGCCTTTCCAAAATTCTGAGTTCCTGCAGTGTTGTTGAATGCATGTTTGGTTCCATTGTTACTAAGATTAGTGTAACCTCCATTGGTAATTTGTGCACCAGTTTTGATGTCAGCCTCACCATAATCTTGATAGCCACACTTGTTGTTAAATGCATCAACAGTTCCATTGTTTCCACCATCGTACCCACCAAAGATGTTGCTGAATAAAACCAGTCATCTTATGATATACGATATGATCTCTGTGAATATATATATAAATAGTTGAAATGCTTAGGTTTGTGTTCCCCCGCGTACTCTGTCGTTGA
TARGET=486,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5881
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.584504
PRIMER_LEFT_SEQUENCE=CCACCAAAGATGTTGCTGAATA
PRIMER_RIGHT_SEQUENCE=GGGAACACAAACCTAAGCATTT
PRIMER_LEFT=424,22
PRIMER_RIGHT=525,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=59.415
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=7.2000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=102
PRIMER_PAIR_PENALTY_1=0.6224
PRIMER_LEFT_1_PENALTY=0.037936
PRIMER_RIGHT_1_PENALTY=0.584504
PRIMER_LEFT_1_SEQUENCE=TGATGTCAGCCTCACCATAATC
PRIMER_RIGHT_1_SEQUENCE=GGGAACACAAACCTAAGCATTT
PRIMER_LEFT_1=344,22
PRIMER_RIGHT_1=525,22
PRIMER_LEFT_1_TM=59.962
PRIMER_RIGHT_1_TM=59.415
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.9000
PRIMER_RIGHT_1_END_STABILITY=7.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=182
PRIMER_PAIR_PENALTY_2=0.6313
PRIMER_LEFT_2_PENALTY=0.046801
PRIMER_RIGHT_2_PENALTY=0.584504
PRIMER_LEFT_2_SEQUENCE=TCTGAGTTCCTGCAGTGTTGTT
PRIMER_RIGHT_2_SEQUENCE=GGGAACACAAACCTAAGCATTT
PRIMER_LEFT_2=254,22
PRIMER_RIGHT_2=525,22
PRIMER_LEFT_2_TM=59.953
PRIMER_RIGHT_2_TM=59.415
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=7.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=272
PRIMER_PAIR_PENALTY_3=0.6313
PRIMER_LEFT_3_PENALTY=0.046801
PRIMER_RIGHT_3_PENALTY=0.584504
PRIMER_LEFT_3_SEQUENCE=TTCTGAGTTCCTGCAGTGTTGT
PRIMER_RIGHT_3_SEQUENCE=GGGAACACAAACCTAAGCATTT
PRIMER_LEFT_3=253,22
PRIMER_RIGHT_3=525,22
PRIMER_LEFT_3_TM=59.953
PRIMER_RIGHT_3_TM=59.415
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=273
PRIMER_PAIR_PENALTY_4=0.7003
PRIMER_LEFT_4_PENALTY=0.115769
PRIMER_RIGHT_4_PENALTY=0.584504
PRIMER_LEFT_4_SEQUENCE=AACCTCCATTGGTAATTTGTGC
PRIMER_RIGHT_4_SEQUENCE=GGGAACACAAACCTAAGCATTT
PRIMER_LEFT_4=314,22
PRIMER_RIGHT_4=525,22
PRIMER_LEFT_4_TM=60.116
PRIMER_RIGHT_4_TM=59.415
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=7.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=212
=
PRIMER_SEQUENCE_ID=SSR_06276 Ps_contig_mira-and-tgicl-ass_franssen_42863
SEQUENCE=GAGTGGCCCATATGGCAAGGATGCTTTTGTTGCATGAACCAAGCTTGGGTTGCCCAAGTAGTCAAGTCCACCCTCGCTAAAGATTTGAGATCCTGCCTTGAACCACACAGCTTCACCGAATTTAACACCGTTGCGAGACAAAAGCTCTGGGAAGACACATCCCAAGGCACCAACAGTAGCCCGATCTGGAGTGGATGACTTCAAGCTCACGGTTCTTGGAGAATGTCTCTGGGTCAGCAGAGAGTCCGGCAGTGTCCCAACCGTAGTCACCGGGGAACTCTCCAGTCAAGTAGGATGGAGACTCACCGGAGAATGGGCCTAAGTACTTAAACRCGGTCTGGTCCGTACCATGGGGTCCTTCCAAGGGAGGGAGGGTTA
TARGET=364,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06277 Ps_contig_mira-and-tgicl-ass_franssen_42892
SEQUENCE=GGAAGGAAAGGAAAGGAAGCTTGGTTCGTGTTTTGGTCCGTCGTCGTTGACCTCGCTCTCGTACGCATCTGATATTGACCTTCTTCGAAAACGAAACGGCTCTCTAGCTCCTTACCGGAAAACGGAGATAGCGGCGGAGTAGAACCAGCGGGGGAAGCCGGAGCTGATCCACTTGGACTCTGGTACCCAGCTGGTTTCACTATCATGATACTACGTGTCACTTTCACTGCACCCTCTAACGAATCCCCACCGTAGGATCTCACGTTTCCACCCTCTGATTCCTTGTCGGAGATAGATCGGGAAAGGAAGGAAGGGTGTTTTCGGAGCTTGCCGAGACCATTCTCCGGCAAAGGTCCGGCGACGGTGTCATCCCACAAGTGATCGAGAAGGCTCATGAGAATATGGAAAGCGCAGACACCCCCGGGTACTNTGCGTT
TARGET=3,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06278 Ps_contig_mira-and-tgicl-ass_franssen_42892
SEQUENCE=GGAAGGAAAGGAAAGGAAGCTTGGTTCGTGTTTTGGTCCGTCGTCGTTGACCTCGCTCTCGTACGCATCTGATATTGACCTTCTTCGAAAACGAAACGGCTCTCTAGCTCCTTACCGGAAAACGGAGATAGCGGCGGAGTAGAACCAGCGGGGGAAGCCGGAGCTGATCCACTTGGACTCTGGTACCCAGCTGGTTTCACTATCATGATACTACGTGTCACTTTCACTGCACCCTCTAACGAATCCCCACCGTAGGATCTCACGTTTCCACCCTCTGATTCCTTGTCGGAGATAGATCGGGAAAGGAAGGAAGGGTGTTTTCGGAGCTTGCCGAGACCATTCTCCGGCAAAGGTCCGGCGACGGTGTCATCCCACAAGTGATCGAGAAGGCTCATGAGAATATGGAAAGCGCAGACACCCCCGGGTACTNTGCGTT
TARGET=303,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3621
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.275745
PRIMER_LEFT_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_RIGHT_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT=217,22
PRIMER_RIGHT=394,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=60.276
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.3840
PRIMER_LEFT_1_PENALTY=0.108206
PRIMER_RIGHT_1_PENALTY=0.275745
PRIMER_LEFT_1_SEQUENCE=ACCCAGCTGGTTTCACTATCAT
PRIMER_RIGHT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_1=184,22
PRIMER_RIGHT_1=394,22
PRIMER_LEFT_1_TM=59.892
PRIMER_RIGHT_1_TM=60.276
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.5000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.6439
PRIMER_LEFT_2_PENALTY=0.368190
PRIMER_RIGHT_2_PENALTY=0.275745
PRIMER_LEFT_2_SEQUENCE=TACCCAGCTGGTTTCACTATCA
PRIMER_RIGHT_2_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_2=183,22
PRIMER_RIGHT_2=394,22
PRIMER_LEFT_2_TM=59.632
PRIMER_RIGHT_2_TM=60.276
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.6994
PRIMER_LEFT_3_PENALTY=0.423703
PRIMER_RIGHT_3_PENALTY=0.275745
PRIMER_LEFT_3_SEQUENCE=ATTCCTTGTCGGAGATAGATCG
PRIMER_RIGHT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_LEFT_3=277,22
PRIMER_RIGHT_3=394,22
PRIMER_LEFT_3_TM=59.576
PRIMER_RIGHT_3_TM=60.276
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=0.7268
PRIMER_LEFT_4_PENALTY=0.086339
PRIMER_RIGHT_4_PENALTY=0.640429
PRIMER_LEFT_4_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_RIGHT_4_SEQUENCE=GTGTCTGCGCTTTCCATATTCT
PRIMER_LEFT_4=217,22
PRIMER_RIGHT_4=417,22
PRIMER_LEFT_4_TM=59.914
PRIMER_RIGHT_4_TM=60.640
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.0000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=201
=
PRIMER_SEQUENCE_ID=SSR_06279 Ps_contig_mira-and-tgicl-ass_franssen_42893
SEQUENCE=CTGTTTGATGTGGATTTCTTAGATTTGCAGGTTTTGAGATCTGAACGTTTTGATTTGGTGTTTTCNTYTNCAGATCTGAGTCGATTGGTTTTCAATTTTCTTGAAATGTCGGATTTAACGAAGGATTTATCAAAAGATTTATCGAAAGGTGATTCGGTTCAAATCAGAGAGGTTTGGAACGATAATCTCGAAGAGGAATTTGCTTTGATCCGTGAAATCGTTGATGATTTTAACTATGTAGCTATGGATACAGAGTTTCCAGGTGTTGTTCTTCGTCCTGTAGGGGTTTTCAAACATATCAATGATTTTAACTACCAGACTTTGAAGGATAACGTTGATATGTTGAAGCTGATTCAGTTAGGTTTAACTTTCTCTGATGAGAATGGGAAGTTACCTACTTGTGGCACTGGAAATCCTTGCATCTGGCAATTTAATTTCCGTGAGTTTAACGTCACCGAAGATATATTCGCTGCTGATTCGGTCGAGCTTTTGCATCAATGTGGAATTGATTTCACTAAGAACAGCGAGCAAGGGATCGACGTGAACCGATTCGGCGAGCTTCTTATGTCGTCTGGGGTTGTGTTAAACGATAACATGCAATGGGTTACTTTCCATAGTGGATATGATTTTGGTTATCTGCTGAAGCTTTTGACTTGCAGAACTTTGCCAGATACGCAAACAGGGTTCTTCGATTTGATCAACATTTATTTTCCGATGCTTTATGATATCAAACATTTGATGAAATTCTGTAACAGTCTTCATGGTGGTTTGAACAAGCTTGCAGAGTTGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTAGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGTAGTTTCTTTTTTACTGTGTACTACTTCTAACCG
TARGET=945,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06280 Ps_contig_mira-and-tgicl-ass_franssen_42923
SEQUENCE=GTCGGGGAAGAGTAAAACGTAGAAACTTTTAGTACCAGAGTTTTGGGAGAGTGATATGGCACAGTGGTCGTTGCTTATCGTTTTGTTCTGCGTCGCCTCCGCCGCCGCCGGATTCAGCTTCCATGACTCCAATCCTATTCGAATGGTCTCCGACGTGGAGGAGCAGTTACTTCAGGTGATCGGAGAAAGTCGTCACGCCGTCTCTTCGCCCGGTTCGCTAACAGATACGGTAAGCGATACGATAG
TARGET=99,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1540
PRIMER_LEFT_PENALTY=0.122257
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=TCGGGGAAGAGTAAAACGTAGA
PRIMER_RIGHT_SEQUENCE=ATTGGAGTCATGGAAGCTGAAT
PRIMER_LEFT=1,22
PRIMER_RIGHT=132,22
PRIMER_LEFT_TM=60.122
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.3085
PRIMER_LEFT_1_PENALTY=0.061818
PRIMER_RIGHT_1_PENALTY=0.246726
PRIMER_LEFT_1_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_1_SEQUENCE=GACTTTCTCCGATCACCTGAAG
PRIMER_LEFT_1=62,22
PRIMER_RIGHT_1=190,22
PRIMER_LEFT_1_TM=60.062
PRIMER_RIGHT_1_TM=60.247
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=129
PRIMER_PAIR_PENALTY_2=0.3235
PRIMER_LEFT_2_PENALTY=0.122257
PRIMER_RIGHT_2_PENALTY=0.201288
PRIMER_LEFT_2_SEQUENCE=TCGGGGAAGAGTAAAACGTAGA
PRIMER_RIGHT_2_SEQUENCE=GACCATTCGAATAGGATTGGAG
PRIMER_LEFT_2=1,22
PRIMER_RIGHT_2=147,22
PRIMER_LEFT_2_TM=60.122
PRIMER_RIGHT_2_TM=59.799
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=0.3235
PRIMER_LEFT_3_PENALTY=0.122257
PRIMER_RIGHT_3_PENALTY=0.201288
PRIMER_LEFT_3_SEQUENCE=TCGGGGAAGAGTAAAACGTAGA
PRIMER_RIGHT_3_SEQUENCE=CCATTCGAATAGGATTGGAGTC
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=145,22
PRIMER_LEFT_3_TM=60.122
PRIMER_RIGHT_3_TM=59.799
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.1000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=0.3637
PRIMER_LEFT_4_PENALTY=0.061818
PRIMER_RIGHT_4_PENALTY=0.301926
PRIMER_LEFT_4_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_4_SEQUENCE=CCTGAAGTAACTGCTCCTCCAC
PRIMER_LEFT_4=62,22
PRIMER_RIGHT_4=175,22
PRIMER_LEFT_4_TM=60.062
PRIMER_RIGHT_4_TM=60.302
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=114
=
PRIMER_SEQUENCE_ID=SSR_06281 Ps_contig_mira-and-tgicl-ass_franssen_43007
SEQUENCE=CGGGGTTTAGGTTTAGGTTTATTTTACGGTTTAGGNTNNGNTAGTGNAGGTAGGAGNATGRTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAATTAGAAAAAATAGTGAGATTAATAGTTGGTTACAGATTTTGTATAATAGNATTTCGGTTTTAAAAAACTTT
TARGET=135,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06282 Ps_contig_mira-and-tgicl-ass_franssen_43013
SEQUENCE=ATGGCCATGTCAACAATTTTATTGATGTTGTTGATGATGCGGACGGTGATCGTGCCTCCCTTCGGGGGAGAGTGACCGGTGATGATCCCAATCTCACAGAGGATGAACGAAACAAAATAATAGAGTTTCTGGAAGCTTTTCCCAGTGAGGATCTCTGAACTGTCAACGCGCTTGCTTGTTGATATGTAAAGTAGTAGGTGCATGTATATAATGTATTCCATGTAATAAAGATTGAATCAGAACCTTCAAATCTCTGTTAATGTCAATGTAATATTAGCATGTGAAGCTTGTTAGGTTCCATGGCAATCAAATGTCTTTTGCATTCTTCAATTAGCACAACATTTCTAATAAACCATGATATTGTACGTAAATAAATGTGAGATGAAGAGTTGCATTCTTCATGACATATATATATAACCAGTAAAATTTTGAAATAAA
TARGET=406,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06283 Ps_contig_mira-and-tgicl-ass_franssen_43023
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTTCTTTTTTTACTGNGTACNCTTA
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06284 Ps_contig_mira-and-tgicl-ass_franssen_43042
SEQUENCE=TGTGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATCTCTGGTGTGTTGTATGGTATTAGGTGTTGAGACAGACTAATAGAAAGAAAGAAATATATTGGTTGTAGATTACAGTATCTTCTTRTGTGTTGTGTAGTTTAYTTTTTACTGTGTACTCTTCTAACG
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06285 Ps_contig_mira-and-tgicl-ass_franssen_43046
SEQUENCE=AATATGTTAGCTTGCTTAAGTCAAATGATCCTGTCGGGTTTAACCTTGTTGATGTTTTGGCTTGGGGTTCTATAGGTCATATTGTTGCTTATTATATCTTGGCCACTTCTAGCAATGGTTATGATCCTAGTTTCTTTGGTTAATTAAGATTACTATGTTGTTCGTTTATGTTGTTTTAATTTGTCTTTTCTTGTCCCTTTTATGTTTATTAAGATGATGATGATGATGATGTATAGTTTATTTGTTATGAGATATTCAACAAGTTAAARTTACCTTTGGTATYAGT
TARGET=213,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06286 Ps_contig_mira-and-tgicl-ass_franssen_43065
SEQUENCE=TTACTTCATTTGACTTTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGACCGTAGCCTCCATGTCCATGACCATAGCCATGACCATAACCTCCACCGTAATATTTGGCATCATTTACTTCATTTGACTTTTCAGCAACTTCCTCTTTGGCATTAGTGGAAGTCTCAGCTAATTCTCTAGCTGAAACCTCGGAGGAAATAACAAGAACCATGGCCAATAGACCAAGGATGAAGGAAAACGTTTCAGGAATCCATTGTACTGAACTCAAGTATGAATTGAATGTGATT
TARGET=80,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7086
PRIMER_LEFT_PENALTY=0.621889
PRIMER_RIGHT_PENALTY=0.086698
PRIMER_LEFT_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_SEQUENCE=AGCTGAGACTTCCACTAATGCC
PRIMER_LEFT=18,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=59.378
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.8754
PRIMER_LEFT_1_PENALTY=0.621889
PRIMER_RIGHT_1_PENALTY=0.253482
PRIMER_LEFT_1_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_1_SEQUENCE=TGAGACTTCCACTAATGCCAAA
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=295,22
PRIMER_LEFT_1_TM=59.378
PRIMER_RIGHT_1_TM=59.747
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.9690
PRIMER_LEFT_2_PENALTY=0.621889
PRIMER_RIGHT_2_PENALTY=0.347129
PRIMER_LEFT_2_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_2_SEQUENCE=CCACTAATGCCAAAGAGGAAGT
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=287,22
PRIMER_LEFT_2_TM=59.378
PRIMER_RIGHT_2_TM=59.653
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=270
PRIMER_PAIR_PENALTY_3=0.9690
PRIMER_LEFT_3_PENALTY=0.621889
PRIMER_RIGHT_3_PENALTY=0.347129
PRIMER_LEFT_3_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_3_SEQUENCE=ACTTCCACTAATGCCAAAGAGG
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=291,22
PRIMER_LEFT_3_TM=59.378
PRIMER_RIGHT_3_TM=59.653
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=1.0018
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=0.379939
PRIMER_LEFT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=AGGTTATGGTCATGGCTATGGT
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=220,22
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=59.620
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_06287 Ps_contig_mira-and-tgicl-ass_franssen_43065
SEQUENCE=TTACTTCATTTGACTTTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGACCGTAGCCTCCATGTCCATGACCATAGCCATGACCATAACCTCCACCGTAATATTTGGCATCATTTACTTCATTTGACTTTTCAGCAACTTCCTCTTTGGCATTAGTGGAAGTCTCAGCTAATTCTCTAGCTGAAACCTCGGAGGAAATAACAAGAACCATGGCCAATAGACCAAGGATGAAGGAAAACGTTTCAGGAATCCATTGTACTGAACTCAAGTATGAATTGAATGTGATT
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7086
PRIMER_LEFT_PENALTY=0.621889
PRIMER_RIGHT_PENALTY=0.086698
PRIMER_LEFT_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_SEQUENCE=AGCTGAGACTTCCACTAATGCC
PRIMER_LEFT=18,22
PRIMER_RIGHT=298,22
PRIMER_LEFT_TM=59.378
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=281
PRIMER_PAIR_PENALTY_1=0.8754
PRIMER_LEFT_1_PENALTY=0.621889
PRIMER_RIGHT_1_PENALTY=0.253482
PRIMER_LEFT_1_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_1_SEQUENCE=TGAGACTTCCACTAATGCCAAA
PRIMER_LEFT_1=18,22
PRIMER_RIGHT_1=295,22
PRIMER_LEFT_1_TM=59.378
PRIMER_RIGHT_1_TM=59.747
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.8000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.9690
PRIMER_LEFT_2_PENALTY=0.621889
PRIMER_RIGHT_2_PENALTY=0.347129
PRIMER_LEFT_2_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_2_SEQUENCE=CCACTAATGCCAAAGAGGAAGT
PRIMER_LEFT_2=18,22
PRIMER_RIGHT_2=287,22
PRIMER_LEFT_2_TM=59.378
PRIMER_RIGHT_2_TM=59.653
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=270
PRIMER_PAIR_PENALTY_3=0.9690
PRIMER_LEFT_3_PENALTY=0.621889
PRIMER_RIGHT_3_PENALTY=0.347129
PRIMER_LEFT_3_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_3_SEQUENCE=ACTTCCACTAATGCCAAAGAGG
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=291,22
PRIMER_LEFT_3_TM=59.378
PRIMER_RIGHT_3_TM=59.653
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=274
PRIMER_PAIR_PENALTY_4=1.0018
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=0.379939
PRIMER_LEFT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=AGGTTATGGTCATGGCTATGGT
PRIMER_LEFT_4=18,22
PRIMER_RIGHT_4=220,22
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=59.620
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=7.8000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_06288 Ps_contig_mira-and-tgicl-ass_franssen_43081
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTTCTTTTTTTACTGTGTAC
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06289 Ps_contig_mira-and-tgicl-ass_franssen_43140
SEQUENCE=GTTCTTAATATTTAAACACTACCAATTTTATTATTATTTTATTTTTACTACACAAAAACACACTTCATAAGATAGAGATAAAAACACTTTATTTTATTACTATTATTACCCAAAAACATACACATACACTTATTATATTATATTATATGTATATGTTTGGAAAGCCCATCAAAGCTGATAGTAATGATTTAATTTCCAATCCTGGCTCCAGTGTTGATCTTGGCCTTTCCAAAATTCTGAGTTCCTGCAGTGTTGTTGAATGCATGTTTGGTTCCATTGTTACTAAGATTAGTGTAACCTCCATTGGTAATTTGTGCACCAGTTTTGATGTCAGCCTCACCATAATCTTGATAGCCACACTTGTTGTTAAATGCATCAACAGTTCCATTGTTTCCACCATCGTACCCAAACCAAAGATTGTTGACTAACGAAAAAAACC
TARGET=131,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06290 Ps_contig_mira-and-tgicl-ass_franssen_43196
SEQUENCE=TGTCTGCGCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCGGACCTTTGCCGGAGAATGGTCTCGGCAAGCTCCGAAAACACCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGAATCAGAGGGTGGAAACGTGAGATCCTACGGTGGGGATTCGTTAGAGGGTGCAGTGAAAGTGACACGTAGTATCATGATAGTGAAACCAGCG
TARGET=104,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3621
PRIMER_LEFT_PENALTY=0.275745
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT=22,22
PRIMER_RIGHT=199,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.6994
PRIMER_LEFT_1_PENALTY=0.275745
PRIMER_RIGHT_1_PENALTY=0.423703
PRIMER_LEFT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_1_SEQUENCE=ATTCCTTGTCGGAGATAGATCG
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=139,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=59.576
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=0.8750
PRIMER_LEFT_2_PENALTY=0.275745
PRIMER_RIGHT_2_PENALTY=0.599276
PRIMER_LEFT_2_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_2_SEQUENCE=GTCACTTTCACTGCACCCTCTA
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=200,22
PRIMER_LEFT_2_TM=60.276
PRIMER_RIGHT_2_TM=59.401
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=179
PRIMER_PAIR_PENALTY_3=0.9862
PRIMER_LEFT_3_PENALTY=0.275745
PRIMER_RIGHT_3_PENALTY=0.710449
PRIMER_LEFT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_3_SEQUENCE=ACTTTCACTGCACCCTCTAACG
PRIMER_LEFT_3=22,22
PRIMER_RIGHT_3=197,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=60.710
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=7.7000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=176
PRIMER_PAIR_PENALTY_4=1.0089
PRIMER_LEFT_4_PENALTY=0.922573
PRIMER_RIGHT_4_PENALTY=0.086339
PRIMER_LEFT_4_SEQUENCE=CTCATGAGCCTTCTCGATCACT
PRIMER_RIGHT_4_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=199,22
PRIMER_LEFT_4_TM=60.923
PRIMER_RIGHT_4_TM=59.914
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=181
=
PRIMER_SEQUENCE_ID=SSR_06291 Ps_contig_mira-and-tgicl-ass_franssen_43212
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTNTTATGTGTTGTGAGTTTCTTTTTTTATGTGTACTACTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06292 Ps_contig_mira-and-tgicl-ass_franssen_43233
SEQUENCE=GGTTTAAAATTGCCCAAAAGAGAATATCATTATTTTTATAATTTAAAGTTCACAATTACAACCATACATCACACATGGGCTGGGCCAGATTCCAACTTAAAAGTAATGAAAAAACTGCCCCATCACCCAACGCACTCACCACCTAATAATAATAATCATTACAACAACACGTTACACTTACAACAACATCCTTTACATACAAAAAGGGAAATAGTTAAAACAAGATAATGATCGAATTTCAAATTAAAAATAAAACCCCACAAAGTTGGTTTGGTTTAATGGTTTTGGCATCAACTATCACAAACCGTTGGATCATATCAACTTGACTTGAAAGGAATGGCTCTGATAACAACTGTGTGGTAAAGTGCTGCAAGTGCTGCACCAATGAATGGTCCAACCCAGAAAATCCATTGATCATCCCAACCAAGATCTTTGTTGAAGATAATTGCAGCACCAAGACTCCTAGCAGGGTTGATACCAGTTCCAGTAATTGGAATAGTAGCCAAGTGAACCAAAAACACAGCGAACCCAATTGGCAAAGGTGCCAAAATAGGAACGTGAGAGTCTCTGGCACTACGTTTGGCATCAGTGGCTGAGAAAACGGTGTAGACAAGAACAAAGGTACCGATAATCTCAGCACCGAGTCCATCTCCTTTTGTGTAACCAGGAGCAACAAAGTTAGCACCACCGTGAACTTTTCCGTAGAAAGCTTTTCCTTCAAAACCCTTAACCACACCAGCACCGCAGATAGCACCAAGAACCTGCATCACGATGTAGAACACTGCTCTTGTTAGAGACAGTTTCCTCGCCAAGAACAAACCGAATGTCACTGCTGGGTTTATGTGACCCCCAGAGATTCCGGCAGTGCAGTAAACGAGGGCGAAGATCATGCCACCAAAAGACCAAGCAATCCCTTGAATACCAACAGTTTTACACTTGGAATCAGATTTGTTGACACCCATGATAGTCAAGATGGTGATGTAGAGAAACAGAAAAGTTGCAACGAACTCGGCTATTCCAGCTCTCCAGAAAGACCATGAAGTGAGTTCAGAAGGCTCAAACAACGGTGCAGGAGGTGGTTCTTTGTAGTCCTTACCGTCGTCATGGCTCTGAGCTGCAATACCGATGGGTTGTCTCTCTGGGAATTTGTTGGCTCCCAGTGAAACATCTTGTTCCTTTGCTTCCATTTCTCACTCTCTTGTTGATGAATGAATAATTGCTATGAATGAATTGGAAGTGGTTGTTGTTGTGGTTCCCCCGAACTCTGGCGTTGACTACC
TARGET=144,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.2669
PRIMER_LEFT_PENALTY=1.134049
PRIMER_RIGHT_PENALTY=0.132857
PRIMER_LEFT_SEQUENCE=ACCATACATCACACATGGGCT
PRIMER_RIGHT_SEQUENCE=TGTGATAGTTGATGCCAAAACC
PRIMER_LEFT=60,21
PRIMER_RIGHT=301,22
PRIMER_LEFT_TM=60.134
PRIMER_RIGHT_TM=59.867
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=10.9000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=1.2669
PRIMER_LEFT_1_PENALTY=1.134049
PRIMER_RIGHT_1_PENALTY=0.132857
PRIMER_LEFT_1_SEQUENCE=ACCATACATCACACATGGGCT
PRIMER_RIGHT_1_SEQUENCE=GGTTTGTGATAGTTGATGCCAA
PRIMER_LEFT_1=60,21
PRIMER_RIGHT_1=305,22
PRIMER_LEFT_1_TM=60.134
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.9000
PRIMER_RIGHT_1_END_STABILITY=10.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=1.2669
PRIMER_LEFT_2_PENALTY=1.134049
PRIMER_RIGHT_2_PENALTY=0.132857
PRIMER_LEFT_2_SEQUENCE=ACCATACATCACACATGGGCT
PRIMER_RIGHT_2_SEQUENCE=GTGATAGTTGATGCCAAAACCA
PRIMER_LEFT_2=60,21
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=60.134
PRIMER_RIGHT_2_TM=59.867
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=10.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=241
PRIMER_PAIR_PENALTY_3=1.3111
PRIMER_LEFT_3_PENALTY=1.134049
PRIMER_RIGHT_3_PENALTY=0.177008
PRIMER_LEFT_3_SEQUENCE=ACCATACATCACACATGGGCT
PRIMER_RIGHT_3_SEQUENCE=TCAAGTTGATATGATCCAACGG
PRIMER_LEFT_3=60,21
PRIMER_RIGHT_3=325,22
PRIMER_LEFT_3_TM=60.134
PRIMER_RIGHT_3_TM=59.823
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=10.9000
PRIMER_RIGHT_3_END_STABILITY=9.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=266
PRIMER_PAIR_PENALTY_4=1.3756
PRIMER_LEFT_4_PENALTY=1.134049
PRIMER_RIGHT_4_PENALTY=0.241544
PRIMER_LEFT_4_SEQUENCE=ACCATACATCACACATGGGCT
PRIMER_RIGHT_4_SEQUENCE=GAGCCATTCCTTTCAAGTCAAG
PRIMER_LEFT_4=60,21
PRIMER_RIGHT_4=342,22
PRIMER_LEFT_4_TM=60.134
PRIMER_RIGHT_4_TM=60.242
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=10.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_06293 Ps_contig_mira-and-tgicl-ass_franssen_43241
SEQUENCE=AACGCAGAGTACGTCGGGGGTGTCTGCGCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCGGACCTTTGCCGGAGAATGGTCTCGGCAAGCTCCGAAAACACCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGAATCAGAGGGTGGAAACGTGAGATCCTACGGGTGGGGATTCGTTAGAGGGTGCAGTGAAAGTGACACGTAGTATCATGATAGTAGAAACCAGCGGGGTACCAGAGTCCAAGTGGATCAGCTCCGGCTTCCCCCGCTGGTTCTACTCCGCCGCTATCTCCGTTTTCCGGAGCTAGAGAGCCGTTTCGTTTTCGAAGAAGGTCAATATCAGATGCGTACGAGAGCGAGGTCAAGACAGGCCAAAACAGACCAAGCTCTTCT
TARGET=124,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3621
PRIMER_LEFT_PENALTY=0.275745
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT=42,22
PRIMER_RIGHT=220,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=179
PRIMER_PAIR_PENALTY_1=0.6994
PRIMER_LEFT_1_PENALTY=0.275745
PRIMER_RIGHT_1_PENALTY=0.423703
PRIMER_LEFT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_1_SEQUENCE=ATTCCTTGTCGGAGATAGATCG
PRIMER_LEFT_1=42,22
PRIMER_RIGHT_1=159,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=59.576
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=118
PRIMER_PAIR_PENALTY_2=0.7268
PRIMER_LEFT_2_PENALTY=0.640429
PRIMER_RIGHT_2_PENALTY=0.086339
PRIMER_LEFT_2_SEQUENCE=GTGTCTGCGCTTTCCATATTCT
PRIMER_RIGHT_2_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT_2=19,22
PRIMER_RIGHT_2=220,22
PRIMER_LEFT_2_TM=60.640
PRIMER_RIGHT_2_TM=59.914
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=202
PRIMER_PAIR_PENALTY_3=0.7528
PRIMER_LEFT_3_PENALTY=0.275745
PRIMER_RIGHT_3_PENALTY=0.477034
PRIMER_LEFT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_3_SEQUENCE=TACCCCGCTGGTTTCTACTATC
PRIMER_LEFT_3=42,22
PRIMER_RIGHT_3=255,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.523
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=214
PRIMER_PAIR_PENALTY_4=0.8750
PRIMER_LEFT_4_PENALTY=0.275745
PRIMER_RIGHT_4_PENALTY=0.599276
PRIMER_LEFT_4_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_4_SEQUENCE=GTCACTTTCACTGCACCCTCTA
PRIMER_LEFT_4=42,22
PRIMER_RIGHT_4=221,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=59.401
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=180
=
PRIMER_SEQUENCE_ID=SSR_06294 Ps_contig_mira-and-tgicl-ass_franssen_43271
SEQUENCE=GTTAGCTTGCTTAAGTCAAATGATCCTGTTCGGGGTTTTAACCYTTGTTGATGTTTTGGCTTGGGGTTCTATAGGTTCATATTGTTGCTTATTATATCTTGGCCACTTCTAGCAATGGTTATGATCCTAGTTTCTTTGGTTAATTAAGATTACTATGTTGTTCGTTTATGTTGTTTTAATTTGTTCTTTTCTTGTCCCTTTTATTGTTTATTAAGATGATGATGATGATGATRATRNNANTAGATGTNTAGTTTATNTGTATGAGATANTNCAACAAGTTAAGTTTCCTTGGTATTAAAAGACTATTTTGTATTTAAGGTAAAAAATT
TARGET=215,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06295 Ps_contig_mira-and-tgicl-ass_franssen_43303
SEQUENCE=CTAAGACTTGAGAAGCATCAGTGGTACCAAACATAGGAAGCTTCCACATTGTCCAGTATCTTCCATCATAGTATCCTGGTGACTTGTTGTGCTCACGGTACACAAATCCTTTCTCCAACTCAAATTCCAAGCATGGAACCCATCCCTTCCTGAGAAGGTATTCAACTTCTTTCTAACAAAYTGATCTCTNGTCAATGGTGGACAAAATAAGGAAAAGAAGTACTACAAAACTTACTTACTTTCCAATTGGAGGCCACACCTGCATGCACTTTACTCTTCCACCATTGCTTGTAATGGAAGTAATGTCAGTGTTGACCTTCTTACTGGANCAGTNATGATTGAGCCGCCGATGGNGCCACCGCGGCCGNTNCACCG
TARGET=230,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06296 Ps_contig_mira-and-tgicl-ass_franssen_43309
SEQUENCE=TTATCTTGATGGGAGCTGTCGAAGGTTACCGTATTGCCGGTGGGCCTCTCGGTGAGGTGGTTGATCCACTTTACCCAGGTGGAAGCTTTGATCCATTGGGCTTAGCTGATGATCCAGAAGCATTCGCAGAATTGAAGGTGAAGGAACTCAAGAACGGTAGATTAGCCATGTTCTCAATGTTTGGATTCTTCGTTCAAGCTATTGTAACTGGAAAGGGTCCTTTGGAAAAACCTTAGCTGATCATCTTGCAGACCCAGTCAAACAAACAAATGCATGGTCCTATGCCACCAAACTTTGTTCCCGG
TARGET=259,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2859
PRIMER_LEFT_PENALTY=0.003589
PRIMER_RIGHT_PENALTY=0.282356
PRIMER_LEFT_SEQUENCE=TAGCCATGTTCTCAATGTTTGG
PRIMER_RIGHT_SEQUENCE=ACAAAGTTTGGTGGCATAGGAC
PRIMER_LEFT=162,22
PRIMER_RIGHT=297,22
PRIMER_LEFT_TM=59.996
PRIMER_RIGHT_TM=60.282
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=7.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.8000
PRIMER_RIGHT_END_STABILITY=7.6000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=0.3989
PRIMER_LEFT_1_PENALTY=0.116582
PRIMER_RIGHT_1_PENALTY=0.282356
PRIMER_LEFT_1_SEQUENCE=GCAGAATTGAAGGTGAAGGAAC
PRIMER_RIGHT_1_SEQUENCE=ACAAAGTTTGGTGGCATAGGAC
PRIMER_LEFT_1=125,22
PRIMER_RIGHT_1=297,22
PRIMER_LEFT_1_TM=60.117
PRIMER_RIGHT_1_TM=60.282
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=7.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=7.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=173
PRIMER_PAIR_PENALTY_2=0.4935
PRIMER_LEFT_2_PENALTY=0.211105
PRIMER_RIGHT_2_PENALTY=0.282356
PRIMER_LEFT_2_SEQUENCE=AAGGTGAAGGAACTCAAGAACG
PRIMER_RIGHT_2_SEQUENCE=ACAAAGTTTGGTGGCATAGGAC
PRIMER_LEFT_2=134,22
PRIMER_RIGHT_2=297,22
PRIMER_LEFT_2_TM=59.789
PRIMER_RIGHT_2_TM=60.282
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=7.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.4000
PRIMER_RIGHT_2_END_STABILITY=7.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=0.5474
PRIMER_LEFT_3_PENALTY=0.265053
PRIMER_RIGHT_3_PENALTY=0.282356
PRIMER_LEFT_3_SEQUENCE=GAGGTGGTTGATCCACTTTACC
PRIMER_RIGHT_3_SEQUENCE=ACAAAGTTTGGTGGCATAGGAC
PRIMER_LEFT_3=53,22
PRIMER_RIGHT_3=297,22
PRIMER_LEFT_3_TM=59.735
PRIMER_RIGHT_3_TM=60.282
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=245
PRIMER_PAIR_PENALTY_4=0.6028
PRIMER_LEFT_4_PENALTY=0.320415
PRIMER_RIGHT_4_PENALTY=0.282356
PRIMER_LEFT_4_SEQUENCE=GAGCTGTCGAAGGTTACCGTAT
PRIMER_RIGHT_4_SEQUENCE=ACAAAGTTTGGTGGCATAGGAC
PRIMER_LEFT_4=12,22
PRIMER_RIGHT_4=297,22
PRIMER_LEFT_4_TM=59.680
PRIMER_RIGHT_4_TM=60.282
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=7.6000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=286
=
PRIMER_SEQUENCE_ID=SSR_06297 Ps_contig_mira-and-tgicl-ass_franssen_43346
SEQUENCE=GAACTCTCCTGTCAAGTAGGATGGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGCTTGATGGGGTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGGTGTGTGTAATTATTG
TARGET=209,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5342
PRIMER_LEFT_PENALTY=0.301926
PRIMER_RIGHT_PENALTY=1.232267
PRIMER_LEFT_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_SEQUENCE=AATAATTACACACACCGATGGC
PRIMER_LEFT=98,22
PRIMER_RIGHT=247,22
PRIMER_LEFT_TM=60.302
PRIMER_RIGHT_TM=58.768
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=150
PRIMER_PAIR_PENALTY_1=1.5342
PRIMER_LEFT_1_PENALTY=0.301926
PRIMER_RIGHT_1_PENALTY=1.232267
PRIMER_LEFT_1_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_1_SEQUENCE=AATAATTACACACACCGATGGC
PRIMER_LEFT_1=99,22
PRIMER_RIGHT_1=247,22
PRIMER_LEFT_1_TM=60.302
PRIMER_RIGHT_1_TM=58.768
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=149
PRIMER_PAIR_PENALTY_2=1.8213
PRIMER_LEFT_2_PENALTY=0.589035
PRIMER_RIGHT_2_PENALTY=1.232267
PRIMER_LEFT_2_SEQUENCE=GAGGAAGCTACTTTCTTGGTGG
PRIMER_RIGHT_2_SEQUENCE=AATAATTACACACACCGATGGC
PRIMER_LEFT_2=85,22
PRIMER_RIGHT_2=247,22
PRIMER_LEFT_2_TM=59.411
PRIMER_RIGHT_2_TM=58.768
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=163
PRIMER_PAIR_PENALTY_3=1.8930
PRIMER_LEFT_3_PENALTY=0.660713
PRIMER_RIGHT_3_PENALTY=1.232267
PRIMER_LEFT_3_SEQUENCE=TTCTTGGTGGTAGCAGACTTCC
PRIMER_RIGHT_3_SEQUENCE=AATAATTACACACACCGATGGC
PRIMER_LEFT_3=97,22
PRIMER_RIGHT_3=247,22
PRIMER_LEFT_3_TM=60.661
PRIMER_RIGHT_3_TM=58.768
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=9.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=2.2710
PRIMER_LEFT_4_PENALTY=1.038744
PRIMER_RIGHT_4_PENALTY=1.232267
PRIMER_LEFT_4_SEQUENCE=GCTACTTTCTTGGTGGTAGCAGA
PRIMER_RIGHT_4_SEQUENCE=AATAATTACACACACCGATGGC
PRIMER_LEFT_4=91,23
PRIMER_RIGHT_4=247,22
PRIMER_LEFT_4_TM=59.961
PRIMER_RIGHT_4_TM=58.768
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_06298 Ps_contig_mira-and-tgicl-ass_franssen_43348
SEQUENCE=TTATATAAAGAGCACACTTTCTTACTTGTCGGGGTGATCCTTCACTACCAAATATCACATTTCAATTCATCTTGAATCAAGTACAATGGATTCCAAAAATGCAATCCTCATCTTTGGTCTATTGGCCATGCTTCTTGTTATTTCCTCACAAGTGTCAGCTAGGGACTTAACTGATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGACACTATGGTCATGGTGGTGGTAGTTACCATGGTGGTGGCGGCAGTTACCACGGTGGACATGGAGGACATGGTGGACATGGTGGACATGGTGGTGTTTCCAACAATGAGGTTGTTGAAACTTCAAATGACGTAAATGATGCCAAATATGGACACTACGGTCATGGTGGTGGTGGTTACCATGGTGGTGGTGGCGGTTACCATGGTGGACATGGAGGACATGGAGGACATGGTGGACACGGTGGTGCTTCAGACAATGGAAACTGATTATATCATCATACATGATGCACATTCAAAGTAATAAGTTTCATGAATGAATAGTTTGGTGTAAAATAAAAGAGGTACAAATAGTCATCATTATGTTGTGGTTATTATGTATTATTTTGTATATGGTGACGAGTTTTATTTCGAGTGAAAGTCATGAATGTGTTATTATTTGTAATATATCAAATATATATTATCGTTGTTTGTACTTTGATTTCTTTTAAGTTTGTACATTGCATAANTACTGATTTGTCTAAGAAAATTGGTCGA
TARGET=382,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4734
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=0.219884
PRIMER_LEFT_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT=359,22
PRIMER_RIGHT=482,22
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=59.780
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=0.7700
PRIMER_LEFT_1_PENALTY=0.550092
PRIMER_RIGHT_1_PENALTY=0.219884
PRIMER_LEFT_1_SEQUENCE=CACTATGGTCATGGTGGTGGTA
PRIMER_RIGHT_1_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT_1=220,22
PRIMER_RIGHT_1=482,22
PRIMER_LEFT_1_TM=60.550
PRIMER_RIGHT_1_TM=59.780
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.7905
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.536975
PRIMER_LEFT_2_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_2_SEQUENCE=AGTTTCCATTGTCTGAAGCACC
PRIMER_LEFT_2=359,22
PRIMER_RIGHT_2=481,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.537
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.8545
PRIMER_LEFT_3_PENALTY=0.634599
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=GATGCCAAATATGGACACTACG
PRIMER_RIGHT_3_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT_3=355,22
PRIMER_RIGHT_3=482,22
PRIMER_LEFT_3_TM=59.365
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=128
PRIMER_PAIR_PENALTY_4=0.9866
PRIMER_LEFT_4_PENALTY=0.253482
PRIMER_RIGHT_4_PENALTY=0.733091
PRIMER_LEFT_4_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_4_SEQUENCE=CTCGAAATAAAACTCGTCACCA
PRIMER_LEFT_4=359,22
PRIMER_RIGHT_4=628,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=59.267
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_06299 Ps_contig_mira-and-tgicl-ass_franssen_43348
SEQUENCE=TTATATAAAGAGCACACTTTCTTACTTGTCGGGGTGATCCTTCACTACCAAATATCACATTTCAATTCATCTTGAATCAAGTACAATGGATTCCAAAAATGCAATCCTCATCTTTGGTCTATTGGCCATGCTTCTTGTTATTTCCTCACAAGTGTCAGCTAGGGACTTAACTGATGAGGTTGTTGAAAAGTCAAATGAAGTAAATGATGCCAAATATGGACACTATGGTCATGGTGGTGGTAGTTACCATGGTGGTGGCGGCAGTTACCACGGTGGACATGGAGGACATGGTGGACATGGTGGACATGGTGGTGTTTCCAACAATGAGGTTGTTGAAACTTCAAATGACGTAAATGATGCCAAATATGGACACTACGGTCATGGTGGTGGTGGTTACCATGGTGGTGGTGGCGGTTACCATGGTGGACATGGAGGACATGGAGGACATGGTGGACACGGTGGTGCTTCAGACAATGGAAACTGATTATATCATCATACATGATGCACATTCAAAGTAATAAGTTTCATGAATGAATAGTTTGGTGTAAAATAAAAGAGGTACAAATAGTCATCATTATGTTGTGGTTATTATGTATTATTTTGTATATGGTGACGAGTTTTATTTCGAGTGAAAGTCATGAATGTGTTATTATTTGTAATATATCAAATATATATTATCGTTGTTTGTACTTTGATTTCTTTTAAGTTTGTACATTGCATAANTACTGATTTGTCTAAGAAAATTGGTCGA
TARGET=400,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4734
PRIMER_LEFT_PENALTY=0.253482
PRIMER_RIGHT_PENALTY=0.219884
PRIMER_LEFT_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT=359,22
PRIMER_RIGHT=482,22
PRIMER_LEFT_TM=59.747
PRIMER_RIGHT_TM=59.780
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=124
PRIMER_PAIR_PENALTY_1=0.7700
PRIMER_LEFT_1_PENALTY=0.550092
PRIMER_RIGHT_1_PENALTY=0.219884
PRIMER_LEFT_1_SEQUENCE=CACTATGGTCATGGTGGTGGTA
PRIMER_RIGHT_1_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT_1=220,22
PRIMER_RIGHT_1=482,22
PRIMER_LEFT_1_TM=60.550
PRIMER_RIGHT_1_TM=59.780
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=263
PRIMER_PAIR_PENALTY_2=0.7905
PRIMER_LEFT_2_PENALTY=0.253482
PRIMER_RIGHT_2_PENALTY=0.536975
PRIMER_LEFT_2_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_2_SEQUENCE=AGTTTCCATTGTCTGAAGCACC
PRIMER_LEFT_2=359,22
PRIMER_RIGHT_2=481,22
PRIMER_LEFT_2_TM=59.747
PRIMER_RIGHT_2_TM=60.537
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=123
PRIMER_PAIR_PENALTY_3=0.8545
PRIMER_LEFT_3_PENALTY=0.634599
PRIMER_RIGHT_3_PENALTY=0.219884
PRIMER_LEFT_3_SEQUENCE=GATGCCAAATATGGACACTACG
PRIMER_RIGHT_3_SEQUENCE=CAGTTTCCATTGTCTGAAGCAC
PRIMER_LEFT_3=355,22
PRIMER_RIGHT_3=482,22
PRIMER_LEFT_3_TM=59.365
PRIMER_RIGHT_3_TM=59.780
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.4000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=128
PRIMER_PAIR_PENALTY_4=0.9866
PRIMER_LEFT_4_PENALTY=0.253482
PRIMER_RIGHT_4_PENALTY=0.733091
PRIMER_LEFT_4_SEQUENCE=CCAAATATGGACACTACGGTCA
PRIMER_RIGHT_4_SEQUENCE=CTCGAAATAAAACTCGTCACCA
PRIMER_LEFT_4=359,22
PRIMER_RIGHT_4=628,22
PRIMER_LEFT_4_TM=59.747
PRIMER_RIGHT_4_TM=59.267
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=270
=
PRIMER_SEQUENCE_ID=SSR_06300 Ps_contig_mira-and-tgicl-ass_franssen_43387
SEQUENCE=AGAATGTCTCTGGGTCAGCAGAGAGTCCGGCAGTGTCCCAACCGTAGTCACCGGGGAACTCTCCAGTCAAGTAGGATGGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCAGAGGAAGCTACTTTCTTGTTGGTAGCAGACTTCCTCACTGGTGGAACCTTGTCAGCTCCCAATTCTTGGCTTGATGGGGTCAGCCTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGTGTGTGTATATTGCAATGAAATTGAAAAGATTGTTATGCTTTTGATAATTGTATTCTTATCAATAGGTACCCCGAC
TARGET=267,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7137
PRIMER_LEFT_PENALTY=0.086339
PRIMER_RIGHT_PENALTY=0.627348
PRIMER_LEFT_SEQUENCE=TTGTTGGTAGCAGACTTCCTCA
PRIMER_RIGHT_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_LEFT=155,22
PRIMER_RIGHT=306,22
PRIMER_LEFT_TM=59.914
PRIMER_RIGHT_TM=59.373
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=0.8205
PRIMER_LEFT_1_PENALTY=0.193133
PRIMER_RIGHT_1_PENALTY=0.627348
PRIMER_LEFT_1_SEQUENCE=GGTAGCAGACTTCCTCACTGGT
PRIMER_RIGHT_1_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_LEFT_1=160,22
PRIMER_RIGHT_1=306,22
PRIMER_LEFT_1_TM=59.807
PRIMER_RIGHT_1_TM=59.373
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=1.0891
PRIMER_LEFT_2_PENALTY=0.461740
PRIMER_RIGHT_2_PENALTY=0.627348
PRIMER_LEFT_2_SEQUENCE=TTGGTAGCAGACTTCCTCACTG
PRIMER_RIGHT_2_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_LEFT_2=158,22
PRIMER_RIGHT_2=306,22
PRIMER_LEFT_2_TM=59.538
PRIMER_RIGHT_2_TM=59.373
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=149
PRIMER_PAIR_PENALTY_3=1.2266
PRIMER_LEFT_3_PENALTY=0.599276
PRIMER_RIGHT_3_PENALTY=0.627348
PRIMER_LEFT_3_SEQUENCE=TGTTGGTAGCAGACTTCCTCAC
PRIMER_RIGHT_3_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_LEFT_3=156,22
PRIMER_RIGHT_3=306,22
PRIMER_LEFT_3_TM=59.401
PRIMER_RIGHT_3_TM=59.373
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=151
PRIMER_PAIR_PENALTY_4=1.3773
PRIMER_LEFT_4_PENALTY=0.749986
PRIMER_RIGHT_4_PENALTY=0.627348
PRIMER_LEFT_4_SEQUENCE=GGGAACTCTCCAGTCAAGTAGG
PRIMER_RIGHT_4_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_LEFT_4=53,22
PRIMER_RIGHT_4=306,22
PRIMER_LEFT_4_TM=59.250
PRIMER_RIGHT_4_TM=59.373
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=254
=
PRIMER_SEQUENCE_ID=SSR_06301 Ps_contig_mira-and-tgicl-ass_franssen_43400
SEQUENCE=TACTGGACAATGTGGAAGCTTCCTATGTTTGGTACCACTGATGCTTCTCAAGTCTTGAAGGAGCTTGATGAAGTTGTTGCCGCTTACCCTCAAGCTTTCGTTCGTATCATCGGTTTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCACACACACCAGAATCCTACTAAGTTTGAGTATTATGGCATTGGAAAAGCTGTTTCTCTTGTACCATTTGTTGTGCTTGTAATTTACTGTGGTTTTTTTTCGGTTTTTGGTTTCGGACTGTAAAATGGAAATGGATGGAGAAGAGTTAATGAATGATATGGTCCTTTTGTTCATTCTCAAATTAGTATTATCTTTTCTTCTTCTTCATTGTTTTGTGTTGAATTTGAAATTTGAAGTAACAAAGGAACTAACAGTATGATAGAC
TARGET=349,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06302 Ps_contig_mira-and-tgicl-ass_franssen_43450
SEQUENCE=CACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCTATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGACCGTAGCCTCCATGTCCATGACCATAGCCATGACCATAACCTCCACCGTAATATTTGGCATCATTTACTTCATTTGACTTTTCAGCAACTTCCTCTTTGGCATTAGTGGAAGTCTCAGCTAATTCTCTAGCTGAAACCTCGGAGGAAATAACAAGAACCATGGCCAATAGACCAAGGATGAGGAAAACGTTTCTGGAATCCATTGTACTGAACTCAAGATGAATTGAAATGTGATTATTTGGTAGTGAAGGACTCCCCCNG
TARGET=20,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06303 Ps_contig_mira-and-tgicl-ass_franssen_43450
SEQUENCE=CACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCTATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGACCGTAGCCTCCATGTCCATGACCATAGCCATGACCATAACCTCCACCGTAATATTTGGCATCATTTACTTCATTTGACTTTTCAGCAACTTCCTCTTTGGCATTAGTGGAAGTCTCAGCTAATTCTCTAGCTGAAACCTCGGAGGAAATAACAAGAACCATGGCCAATAGACCAAGGATGAGGAAAACGTTTCTGGAATCCATTGTACTGAACTCAAGATGAATTGAAATGTGATTATTTGGTAGTGAAGGACTCCCCCNG
TARGET=44,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.6415
PRIMER_LEFT_PENALTY=2.554765
PRIMER_RIGHT_PENALTY=0.086698
PRIMER_LEFT_SEQUENCE=ATGACCGTAACCACCACCAC
PRIMER_RIGHT_SEQUENCE=AGCTGAGACTTCCACTAATGCC
PRIMER_LEFT=10,20
PRIMER_RIGHT=239,22
PRIMER_LEFT_TM=60.555
PRIMER_RIGHT_TM=59.913
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=9.6000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=230
PRIMER_PAIR_PENALTY_1=2.8038
PRIMER_LEFT_1_PENALTY=2.554765
PRIMER_RIGHT_1_PENALTY=0.249074
PRIMER_LEFT_1_SEQUENCE=ATGACCGTAACCACCACCAC
PRIMER_RIGHT_1_SEQUENCE=GTCTATTGGCCATGGTTCTTGT
PRIMER_LEFT_1=10,20
PRIMER_RIGHT_1=291,22
PRIMER_LEFT_1_TM=60.555
PRIMER_RIGHT_1_TM=60.249
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=282
PRIMER_PAIR_PENALTY_2=2.8082
PRIMER_LEFT_2_PENALTY=2.554765
PRIMER_RIGHT_2_PENALTY=0.253482
PRIMER_LEFT_2_SEQUENCE=ATGACCGTAACCACCACCAC
PRIMER_RIGHT_2_SEQUENCE=TGAGACTTCCACTAATGCCAAA
PRIMER_LEFT_2=10,20
PRIMER_RIGHT_2=236,22
PRIMER_LEFT_2_TM=60.555
PRIMER_RIGHT_2_TM=59.747
PRIMER_LEFT_2_GC_PERCENT=55.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=8.8000
PRIMER_PAIR_2_COMPL_ANY=2.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=227
PRIMER_PAIR_PENALTY_3=2.8113
PRIMER_LEFT_3_PENALTY=2.554765
PRIMER_RIGHT_3_PENALTY=0.256503
PRIMER_LEFT_3_SEQUENCE=ATGACCGTAACCACCACCAC
PRIMER_RIGHT_3_SEQUENCE=CTAATGCCAAAGAGGAAGTTGC
PRIMER_LEFT_3=10,20
PRIMER_RIGHT_3=225,22
PRIMER_LEFT_3_TM=60.555
PRIMER_RIGHT_3_TM=60.257
PRIMER_LEFT_3_GC_PERCENT=55.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=216
PRIMER_PAIR_PENALTY_4=2.8665
PRIMER_LEFT_4_PENALTY=2.554765
PRIMER_RIGHT_4_PENALTY=0.311784
PRIMER_LEFT_4_SEQUENCE=ATGACCGTAACCACCACCAC
PRIMER_RIGHT_4_SEQUENCE=TTCCTCATCCTTGGTCTATTGG
PRIMER_LEFT_4=10,20
PRIMER_RIGHT_4=304,22
PRIMER_LEFT_4_TM=60.555
PRIMER_RIGHT_4_TM=60.312
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=7.6000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=295
=
PRIMER_SEQUENCE_ID=SSR_06304 Ps_contig_mira-and-tgicl-ass_franssen_43487
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTNTTATGTGTTGTGTAGTTTCTTTTTTTACTGTGTACACTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06305 Ps_contig_mira-and-tgicl-ass_franssen_43544
SEQUENCE=CCGTTAACCATYGGTRRTTNTGNTGCCATCACGAGTTTTGATGTCAGCCTCACCATAATCTTGATAGCCACACTTGTTGTTAAATGCACCAACAGTTCCATTGTTTCCACCATCGTACCCACCAAAGATGTTGCTGAATAAACCAGTCATCTTATGATATACGATATGATCTCTGTGAATATATATATAAATAGTTGAAATGCTTAGGTTTGTGTTCATC
TARGET=179,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06306 Ps_contig_mira-and-tgicl-ass_franssen_43602
SEQUENCE=TCAAGTAGGATGGAGACTCACCGGAGAATGGGCCTTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGCTTGATGGGGTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGTGTGTGTATATTAGACAAATACCCGA
TARGET=199,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9407
PRIMER_LEFT_PENALTY=0.301926
PRIMER_RIGHT_PENALTY=0.638796
PRIMER_LEFT_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_SEQUENCE=TAATATACACACACGATGGCCG
PRIMER_LEFT=88,22
PRIMER_RIGHT=235,22
PRIMER_LEFT_TM=60.302
PRIMER_RIGHT_TM=60.639
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=0.9407
PRIMER_LEFT_1_PENALTY=0.301926
PRIMER_RIGHT_1_PENALTY=0.638796
PRIMER_LEFT_1_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_1_SEQUENCE=TAATATACACACACGATGGCCG
PRIMER_LEFT_1=89,22
PRIMER_RIGHT_1=235,22
PRIMER_LEFT_1_TM=60.302
PRIMER_RIGHT_1_TM=60.639
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=1.2278
PRIMER_LEFT_2_PENALTY=0.589035
PRIMER_RIGHT_2_PENALTY=0.638796
PRIMER_LEFT_2_SEQUENCE=GAGGAAGCTACTTTCTTGGTGG
PRIMER_RIGHT_2_SEQUENCE=TAATATACACACACGATGGCCG
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=235,22
PRIMER_LEFT_2_TM=59.411
PRIMER_RIGHT_2_TM=60.639
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=1.2995
PRIMER_LEFT_3_PENALTY=0.660713
PRIMER_RIGHT_3_PENALTY=0.638796
PRIMER_LEFT_3_SEQUENCE=TTCTTGGTGGTAGCAGACTTCC
PRIMER_RIGHT_3_SEQUENCE=TAATATACACACACGATGGCCG
PRIMER_LEFT_3=87,22
PRIMER_RIGHT_3=235,22
PRIMER_LEFT_3_TM=60.661
PRIMER_RIGHT_3_TM=60.639
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=12.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=1.6775
PRIMER_LEFT_4_PENALTY=1.038744
PRIMER_RIGHT_4_PENALTY=0.638796
PRIMER_LEFT_4_SEQUENCE=GCTACTTTCTTGGTGGTAGCAGA
PRIMER_RIGHT_4_SEQUENCE=TAATATACACACACGATGGCCG
PRIMER_LEFT_4=81,23
PRIMER_RIGHT_4=235,22
PRIMER_LEFT_4_TM=59.961
PRIMER_RIGHT_4_TM=60.639
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=12.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_06307 Ps_contig_mira-and-tgicl-ass_franssen_43607
SEQUENCE=CTTAGGYNNNTGCAAAGTATATATACTATGATTCTACATATAAGATCAAACACAAGGTAAATAAATGCATCGAATATTAGAACATAGAATTTAATTTGTCATGGAGTAACAGACTTAGGAAAAAGCTTCATTTGCAACTTTCTAGCATCTCAGTTCAATTATCTACATGAAATGTTTTCGATTACAAGCCAAGAGCCCCGAGGGGTGTCTTCCCTTGTCCAGCTTCAAAGTAGAAAATCAGCATAGCTAACATAGCAAGCCTAGCATGCTTAATCTCAGCGAGTTTCAATCTCTCTAACTTGTCTGTATCTGGAATGTAGACTCCGTTTTCGATGGTACCAGCGAGTGACAGAGGATCGAAGAATTTTCCACCGGGGTAGCCTTGTTCTCCGGTTGAGTTGACGAAGTTCTCCGCGGTTTTAGACCATGGAGTGGCCCATTCGACCGACTGTGAGTCCGGGTTGAAGAAATCAACCCATCTCTTGCTCTCTACCCATCCCATGAGAATCAACTGTGTGCCTAGAAGAGTACCGAACGAGAATGGAGCGATTGCGTTAGGGTCAGCTCCGGCCTCGAACCATGGAACTCCACTCCATGCCTGACCAACAAAGATTCCTAGAACTGCAGCCATTGCCCATCTTCCATGAATGAGCTCAGCTTCTCTGTACCATTTGAGAAATGCTGGGTCCTTGCCAAGGCCTAGTGGGTCGAAACCAAAGTCACCCGGTAGCGATCCGTCGAGCCATTCGGGGTCGACGAGGTTTCCACCGAAACTAACACCAGGGATCCATGATTTCTTAGGTGCAGAAGCAGCAGCAGCAACAATAAGTCTTTTAGGACTAACAGCTGCAACACCAACTTTGCTTCCAATGGCAGTAGCTAGCAAGGTTTGGCTCCTCTTTCCACCAGATAAGAAGGAAGATCCCAAACCATTCAACACAGCACCAGATGTTGCAGCTGCCATTTCTATTCTCTTCACACTCTAAAGAAGTTTCACTAAAGATGTATAATGAGATAATAGTAATAGAAGTTTGCTAGATAGTATTTTATGTCCCCGACGATAATGTGAGGGGTTT
TARGET=809,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06308 Ps_contig_mira-and-tgicl-ass_franssen_43659
SEQUENCE=CAACGTCGGGGATTCCATTCCATTCCATTCACTCATCAAACCAAAAACAATGGCAGCATCAGCATCATCCATGATCTCAACAAACGCCCTAACATCCACACTCCAAAAACACCAAACACACAACCTCAAACCATCAAACCTATGTTTCCAAGGCTTCAAACCCTTAACAAGATCAACAAAACTGACACAGAACAAAAAGAGATTCTCATCCCTTGTTGTCAAAGCTGAACTCAACCCTTCACTTGTGATAAGCTTAAGCACTGGTCTGTCATTTGTTTTTAGGAAGGTTTGTGTTCTTCAACTTCCAAAGAGAGAATGTTGCAAAAACAAGGTTTACCTGAACAGAATGGTGTGACACACTTTGA
TARGET=12,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06309 Ps_contig_mira-and-tgicl-ass_franssen_43730
SEQUENCE=TGCAAGAAGCCTAGGTCCAAAAGGGTCAGACCCACACAAGGCAGCAGTTATTGGAGACACCATTGGAGACCCATTGAAGGATACATCTGGACCTTCACTTAACATCCTTATCAAGCTGATGGCAGTTGAGTCACTGGTGTTTGCTCCTTTCTTTGCAACCCACGGTGGTCTCCTCTTCAAGTTTTGGTAATAGAAGAGCATCGTCCATCCCACCCACCAACCAACCAACCATGGCGTCTCTCTCTTTTTTGTTAGTTTGATGGCAACTTGTTTTTGTTTTTTATTATTATCTTATTATTATCCCCCCTAATCCCCTCTTGTTAGATTTGAGATACTTAATTAGGCCGAATCTTTTCCGCGTGGTCATTTTTCTTGTAAAAGTCCAAGTTGATACGATGGCGATCATATTTTACTGTCTTGCCCCGCGTGGTCATTTTTCAATATTCCTTTCACATCTCCAATTTTACATTATTTTCTANTGCTGCCTGCTTATAAATTT
TARGET=216,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5647
PRIMER_LEFT_PENALTY=0.184188
PRIMER_RIGHT_PENALTY=0.380537
PRIMER_LEFT_SEQUENCE=GTTGAGTCACTGGTGTTTGCTC
PRIMER_RIGHT_SEQUENCE=GCCATCGTATCAACTTGGACTT
PRIMER_LEFT=124,22
PRIMER_RIGHT=399,22
PRIMER_LEFT_TM=59.816
PRIMER_RIGHT_TM=60.381
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=276
PRIMER_PAIR_PENALTY_1=0.5899
PRIMER_LEFT_1_PENALTY=0.209343
PRIMER_RIGHT_1_PENALTY=0.380537
PRIMER_LEFT_1_SEQUENCE=AGTCACTGGTGTTTGCTCCTTT
PRIMER_RIGHT_1_SEQUENCE=GCCATCGTATCAACTTGGACTT
PRIMER_LEFT_1=128,22
PRIMER_RIGHT_1=399,22
PRIMER_LEFT_1_TM=60.209
PRIMER_RIGHT_1_TM=60.381
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=272
PRIMER_PAIR_PENALTY_2=0.7226
PRIMER_LEFT_2_PENALTY=0.074466
PRIMER_RIGHT_2_PENALTY=0.648142
PRIMER_LEFT_2_SEQUENCE=ACCCATTGAAGGATACATCTGG
PRIMER_RIGHT_2_SEQUENCE=TCTAACAAGAGGGGATTAGGGG
PRIMER_LEFT_2=68,22
PRIMER_RIGHT_2=324,22
PRIMER_LEFT_2_TM=60.074
PRIMER_RIGHT_2_TM=60.648
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=10.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=257
PRIMER_PAIR_PENALTY_3=0.7234
PRIMER_LEFT_3_PENALTY=0.184188
PRIMER_RIGHT_3_PENALTY=0.539189
PRIMER_LEFT_3_SEQUENCE=GTTGAGTCACTGGTGTTTGCTC
PRIMER_RIGHT_3_SEQUENCE=TATGATCGCCATCGTATCAACT
PRIMER_LEFT_3=124,22
PRIMER_RIGHT_3=406,22
PRIMER_LEFT_3_TM=59.816
PRIMER_RIGHT_3_TM=59.461
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.7485
PRIMER_LEFT_4_PENALTY=0.209343
PRIMER_RIGHT_4_PENALTY=0.539189
PRIMER_LEFT_4_SEQUENCE=AGTCACTGGTGTTTGCTCCTTT
PRIMER_RIGHT_4_SEQUENCE=TATGATCGCCATCGTATCAACT
PRIMER_LEFT_4=128,22
PRIMER_RIGHT_4=406,22
PRIMER_LEFT_4_TM=60.209
PRIMER_RIGHT_4_TM=59.461
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.5000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=279
=
PRIMER_SEQUENCE_ID=SSR_06310 Ps_contig_mira-and-tgicl-ass_franssen_43736
SEQUENCE=CGGGAAGTCAGGGTATCGACGCAGAGTACGCGGGGATCATCATCATCACATTCAAGTTCAAGAAAGAAAGAAACTACTATGGCTCAAGCTATGGCATCATCAATGGCTGGTTGTTTACGTGGTTGTTCATCTCAAACTGTGTTAGAAGGAAGTCTTCAATTTAGTGGACCAAACAGGTTGAGTTTGTTGCACGGAAACACCAACAACGTTAACAAGGTTACAACACGTTCATCAGTGACGGTTAGAGCTCAACAACAGGAGAGTAGTCGAAGGGCTGTGATTGGTCTTGTTGCTACTGGTTTGGTTTCTTCTTCTTTTGTTCAAGCTGTTCTTGCTGAGGCTATTCCTATTAAAGTTGGTGGCCCTCCTCCACTTTCTGGTGGCCTTCCTGGAACATTGAACTCTGATGAAGCAAGAGACCTAAAGCTACCATTGAAAGAAAGGTTCTTTATTCAACCATTGGCACCTACTGAAGCAGCAGCAAGAACTAAGGAATCAGCGAAGGAAATTGTTGCTGCTAAGAAGTTCATTGACCAGAAAGCTTGGCCGTTTCTTCAGAACGATCTCCGTCTCAGGGCTGGCTATCTTCGATATGACCTTAAAACTATCATCTCTTCAAAGCCTAAGGACCAGAAACAATCCCTCAAGGAACTCACCGATAAGCTCTTCCAGGATATCAGCAATTTGGATCATGCAGCAAAAATAAAGAGTCCCTCAGAAGCAGAGAAGTACTATGCTATAGCTGTATCTACTCTGAATGATGTTCTTAGCAAAATTGCTTAATGTGAATGATTTCTTGCTTTCTTGTGTAAGCTTTGTCTATGGTTTTTGTAAGACATTTTATGTTAAGTACTATTTGAACAAAATCAAATGAGAAAAATTGCTATAAGCAAGCAGGAAATTATCATTTGTTAATGCATTTCTTAATTGAAGTTGTTTTCTTAGTTTTAGTAGAGCCAAATATTTAACCATTTACTCAATCAATAAAATAGAAAGGGATGTGTTTAAAAGTA
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6364
PRIMER_LEFT_PENALTY=1.574392
PRIMER_RIGHT_PENALTY=0.061961
PRIMER_LEFT_SEQUENCE=GTCAGGGTATCGACGCAGAGTA
PRIMER_RIGHT_SEQUENCE=AACTCAACCTGTTTGGTCCACT
PRIMER_LEFT=6,22
PRIMER_RIGHT=183,22
PRIMER_LEFT_TM=61.574
PRIMER_RIGHT_TM=59.938
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=1.7072
PRIMER_LEFT_1_PENALTY=1.574392
PRIMER_RIGHT_1_PENALTY=0.132857
PRIMER_LEFT_1_SEQUENCE=GTCAGGGTATCGACGCAGAGTA
PRIMER_RIGHT_1_SEQUENCE=GTAAACAACCAGCCATTGATGA
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=117,22
PRIMER_LEFT_1_TM=61.574
PRIMER_RIGHT_1_TM=59.867
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=112
PRIMER_PAIR_PENALTY_2=1.7425
PRIMER_LEFT_2_PENALTY=1.574392
PRIMER_RIGHT_2_PENALTY=0.168114
PRIMER_LEFT_2_SEQUENCE=GTCAGGGTATCGACGCAGAGTA
PRIMER_RIGHT_2_SEQUENCE=CCAGTAGCAACAAGACCAATCA
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=298,22
PRIMER_LEFT_2_TM=61.574
PRIMER_RIGHT_2_TM=60.168
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=293
PRIMER_PAIR_PENALTY_3=1.7677
PRIMER_LEFT_3_PENALTY=1.574392
PRIMER_RIGHT_3_PENALTY=0.193337
PRIMER_LEFT_3_SEQUENCE=GTCAGGGTATCGACGCAGAGTA
PRIMER_RIGHT_3_SEQUENCE=TGTTAACGTTGTTGGTGTTTCC
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=213,22
PRIMER_LEFT_3_TM=61.574
PRIMER_RIGHT_3_TM=59.807
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=208
PRIMER_PAIR_PENALTY_4=1.7777
PRIMER_LEFT_4_PENALTY=1.574392
PRIMER_RIGHT_4_PENALTY=0.203324
PRIMER_LEFT_4_SEQUENCE=GTCAGGGTATCGACGCAGAGTA
PRIMER_RIGHT_4_SEQUENCE=CTCTAACCGTCACTGATGAACG
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=61.574
PRIMER_RIGHT_4_TM=59.797
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.4000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=241
=
PRIMER_SEQUENCE_ID=SSR_06311 Ps_contig_mira-and-tgicl-ass_franssen_43736
SEQUENCE=CGGGAAGTCAGGGTATCGACGCAGAGTACGCGGGGATCATCATCATCACATTCAAGTTCAAGAAAGAAAGAAACTACTATGGCTCAAGCTATGGCATCATCAATGGCTGGTTGTTTACGTGGTTGTTCATCTCAAACTGTGTTAGAAGGAAGTCTTCAATTTAGTGGACCAAACAGGTTGAGTTTGTTGCACGGAAACACCAACAACGTTAACAAGGTTACAACACGTTCATCAGTGACGGTTAGAGCTCAACAACAGGAGAGTAGTCGAAGGGCTGTGATTGGTCTTGTTGCTACTGGTTTGGTTTCTTCTTCTTTTGTTCAAGCTGTTCTTGCTGAGGCTATTCCTATTAAAGTTGGTGGCCCTCCTCCACTTTCTGGTGGCCTTCCTGGAACATTGAACTCTGATGAAGCAAGAGACCTAAAGCTACCATTGAAAGAAAGGTTCTTTATTCAACCATTGGCACCTACTGAAGCAGCAGCAAGAACTAAGGAATCAGCGAAGGAAATTGTTGCTGCTAAGAAGTTCATTGACCAGAAAGCTTGGCCGTTTCTTCAGAACGATCTCCGTCTCAGGGCTGGCTATCTTCGATATGACCTTAAAACTATCATCTCTTCAAAGCCTAAGGACCAGAAACAATCCCTCAAGGAACTCACCGATAAGCTCTTCCAGGATATCAGCAATTTGGATCATGCAGCAAAAATAAAGAGTCCCTCAGAAGCAGAGAAGTACTATGCTATAGCTGTATCTACTCTGAATGATGTTCTTAGCAAAATTGCTTAATGTGAATGATTTCTTGCTTTCTTGTGTAAGCTTTGTCTATGGTTTTTGTAAGACATTTTATGTTAAGTACTATTTGAACAAAATCAAATGAGAAAAATTGCTATAAGCAAGCAGGAAATTATCATTTGTTAATGCATTTCTTAATTGAAGTTGTTTTCTTAGTTTTAGTAGAGCCAAATATTTAACCATTTACTCAATCAATAAAATAGAAAGGGATGTGTTTAAAAGTA
TARGET=60,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6194
PRIMER_LEFT_PENALTY=0.557487
PRIMER_RIGHT_PENALTY=0.061961
PRIMER_LEFT_SEQUENCE=GGGATCATCATCATCACATTCA
PRIMER_RIGHT_SEQUENCE=AACTCAACCTGTTTGGTCCACT
PRIMER_LEFT=32,22
PRIMER_RIGHT=183,22
PRIMER_LEFT_TM=60.557
PRIMER_RIGHT_TM=59.938
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=152
PRIMER_PAIR_PENALTY_1=0.7256
PRIMER_LEFT_1_PENALTY=0.557487
PRIMER_RIGHT_1_PENALTY=0.168114
PRIMER_LEFT_1_SEQUENCE=GGGATCATCATCATCACATTCA
PRIMER_RIGHT_1_SEQUENCE=CCAGTAGCAACAAGACCAATCA
PRIMER_LEFT_1=32,22
PRIMER_RIGHT_1=298,22
PRIMER_LEFT_1_TM=60.557
PRIMER_RIGHT_1_TM=60.168
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=267
PRIMER_PAIR_PENALTY_2=0.7508
PRIMER_LEFT_2_PENALTY=0.557487
PRIMER_RIGHT_2_PENALTY=0.193337
PRIMER_LEFT_2_SEQUENCE=GGGATCATCATCATCACATTCA
PRIMER_RIGHT_2_SEQUENCE=TGTTAACGTTGTTGGTGTTTCC
PRIMER_LEFT_2=32,22
PRIMER_RIGHT_2=213,22
PRIMER_LEFT_2_TM=60.557
PRIMER_RIGHT_2_TM=59.807
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=182
PRIMER_PAIR_PENALTY_3=0.7608
PRIMER_LEFT_3_PENALTY=0.557487
PRIMER_RIGHT_3_PENALTY=0.203343
PRIMER_LEFT_3_SEQUENCE=GGGATCATCATCATCACATTCA
PRIMER_RIGHT_3_SEQUENCE=CAAACCAGTAGCAACAAGACCA
PRIMER_LEFT_3=32,22
PRIMER_RIGHT_3=302,22
PRIMER_LEFT_3_TM=60.557
PRIMER_RIGHT_3_TM=60.203
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=271
PRIMER_PAIR_PENALTY_4=0.8515
PRIMER_LEFT_4_PENALTY=0.557487
PRIMER_RIGHT_4_PENALTY=0.294053
PRIMER_LEFT_4_SEQUENCE=GGGATCATCATCATCACATTCA
PRIMER_RIGHT_4_SEQUENCE=CCCTTCGACTACTCTCCTGTTG
PRIMER_LEFT_4=32,22
PRIMER_RIGHT_4=273,22
PRIMER_LEFT_4_TM=60.557
PRIMER_RIGHT_4_TM=60.294
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.9000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=242
=
PRIMER_SEQUENCE_ID=SSR_06312 Ps_contig_mira-and-tgicl-ass_franssen_43746
SEQUENCE=TTTACGAGAAATATGGCACTTAATAATGAAAGAGAACTTGAAAATAACTAATGATACACAAAATAGAAAAACAAACACATAGGAATCGCATACATACATAGGAAATAATAAATAAAAATAAAAAGACACAAAAGGGATACAAATTACACCCTTGAAACACAAACATGGATATCTACAATTTCATGGTCCCTTCTTTTGTTTCTTTGTTTTCTCCAAATCCCACTACAGCTATAACTGAAAACGAAAAGAGGAAGAAAAAAACCCAAAAGGACCACTTAACTTTCACTCATCAACATTATTCTTGTGTGGAAATTAACACCATATCTTCCTTGGTAAATTAAGCAATTTAGCTAGCTTAATTAATTAATCAATTAAGCGTTGCTCCTGAATGATCCAAGAGCTTTAATTGCTGATCCTCTTAGAATGTATTGGTGGTAAATTGCAGCCACGGTTGCTCCAATTATTGGTCCAACCCAGAAAACCCACTGATCATCCCAAATTTTCCCCTGGTTTAAGATCACAGCGGATCCGAAACTTCTTGCCGGGTTAATACCGGTACCGGTGATCGGAATAGTAGCCAAGTGAACCATGAAAACAGCAAATCCAATAGGTAATGGTGCCAAAACAGGAACATGAGAGTCCCTAGCGTTTCTCTTAGGATCAGTTGCAGAGAAGACAGTGTAGACAAGAACAAAGGTACCAATAATCTCAGCACCTAAAGCTGTACCTTTGTTGTAACCATCATGGATAAAGTTGGCACCACCTCCATACCTGTCAAAGAATGATTTCTGAAAACCCTTGGCCAGTCCGGTACCACACACTGCTCCTGCACACTGTGCTGCCATGTAGAATACTGCCCTTAACAAGGACACCTTGCGTCCCACGAATAGCCCGAACGTCACCGCAGGGTTTATGTGTCCTCCAGAGATACCGGCGGTGCAGTAAACAAGGATGAAAATCATGCCACCAAAGGCCCAGGCAATACCCAAAATGCCAACACCGTCGCAGTCGGTACCACCGGCGTTAGCATCAGTCTGGTGGCTGTAACCAATGATGGTTAAAATAGTAACATATAGGAAGAGAAGTGTTGCTACGAATTCAGCTATAAGAGCTCTGTAAAAGGACCACTTTGTTAACTCATCGAGGTCTATCAATGGTGCTGGAGGTGGATCTTGGTAGTCTTTTGCTGAGTATTCACCTTGTTCTTGGACCTCAACATCTTTAGCCATGGCTCTGTTTTTTCTGAAGTGGGAGAGAGTAGTGAGTGTCCCCCGACAAACCT
TARGET=89,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06313 Ps_contig_mira-and-tgicl-ass_franssen_43746
SEQUENCE=TTTACGAGAAATATGGCACTTAATAATGAAAGAGAACTTGAAAATAACTAATGATACACAAAATAGAAAAACAAACACATAGGAATCGCATACATACATAGGAAATAATAAATAAAAATAAAAAGACACAAAAGGGATACAAATTACACCCTTGAAACACAAACATGGATATCTACAATTTCATGGTCCCTTCTTTTGTTTCTTTGTTTTCTCCAAATCCCACTACAGCTATAACTGAAAACGAAAAGAGGAAGAAAAAAACCCAAAAGGACCACTTAACTTTCACTCATCAACATTATTCTTGTGTGGAAATTAACACCATATCTTCCTTGGTAAATTAAGCAATTTAGCTAGCTTAATTAATTAATCAATTAAGCGTTGCTCCTGAATGATCCAAGAGCTTTAATTGCTGATCCTCTTAGAATGTATTGGTGGTAAATTGCAGCCACGGTTGCTCCAATTATTGGTCCAACCCAGAAAACCCACTGATCATCCCAAATTTTCCCCTGGTTTAAGATCACAGCGGATCCGAAACTTCTTGCCGGGTTAATACCGGTACCGGTGATCGGAATAGTAGCCAAGTGAACCATGAAAACAGCAAATCCAATAGGTAATGGTGCCAAAACAGGAACATGAGAGTCCCTAGCGTTTCTCTTAGGATCAGTTGCAGAGAAGACAGTGTAGACAAGAACAAAGGTACCAATAATCTCAGCACCTAAAGCTGTACCTTTGTTGTAACCATCATGGATAAAGTTGGCACCACCTCCATACCTGTCAAAGAATGATTTCTGAAAACCCTTGGCCAGTCCGGTACCACACACTGCTCCTGCACACTGTGCTGCCATGTAGAATACTGCCCTTAACAAGGACACCTTGCGTCCCACGAATAGCCCGAACGTCACCGCAGGGTTTATGTGTCCTCCAGAGATACCGGCGGTGCAGTAAACAAGGATGAAAATCATGCCACCAAAGGCCCAGGCAATACCCAAAATGCCAACACCGTCGCAGTCGGTACCACCGGCGTTAGCATCAGTCTGGTGGCTGTAACCAATGATGGTTAAAATAGTAACATATAGGAAGAGAAGTGTTGCTACGAATTCAGCTATAAGAGCTCTGTAAAAGGACCACTTTGTTAACTCATCGAGGTCTATCAATGGTGCTGGAGGTGGATCTTGGTAGTCTTTTGCTGAGTATTCACCTTGTTCTTGGACCTCAACATCTTTAGCCATGGCTCTGTTTTTTCTGAAGTGGGAGAGAGTAGTGAGTGTCCCCCGACAAACCT
TARGET=356,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2538
PRIMER_LEFT_PENALTY=0.149516
PRIMER_RIGHT_PENALTY=0.104238
PRIMER_LEFT_SEQUENCE=CTTTGTTTTCTCCAAATCCCAC
PRIMER_RIGHT_SEQUENCE=GCTGCAATTTACCACCAATACA
PRIMER_LEFT=201,22
PRIMER_RIGHT=445,22
PRIMER_LEFT_TM=59.850
PRIMER_RIGHT_TM=59.896
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.4000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=245
PRIMER_PAIR_PENALTY_1=0.4364
PRIMER_LEFT_1_PENALTY=0.332137
PRIMER_RIGHT_1_PENALTY=0.104238
PRIMER_LEFT_1_SEQUENCE=TCATGGTCCCTTCTTTTGTTTC
PRIMER_RIGHT_1_SEQUENCE=GCTGCAATTTACCACCAATACA
PRIMER_LEFT_1=180,22
PRIMER_RIGHT_1=445,22
PRIMER_LEFT_1_TM=60.332
PRIMER_RIGHT_1_TM=59.896
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.5870
PRIMER_LEFT_2_PENALTY=0.482784
PRIMER_RIGHT_2_PENALTY=0.104238
PRIMER_LEFT_2_SEQUENCE=TTTCTCCAAATCCCACTACAGC
PRIMER_RIGHT_2_SEQUENCE=GCTGCAATTTACCACCAATACA
PRIMER_LEFT_2=207,22
PRIMER_RIGHT_2=445,22
PRIMER_LEFT_2_TM=60.483
PRIMER_RIGHT_2_TM=59.896
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.6000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=239
PRIMER_PAIR_PENALTY_3=0.6048
PRIMER_LEFT_3_PENALTY=0.500551
PRIMER_RIGHT_3_PENALTY=0.104238
PRIMER_LEFT_3_SEQUENCE=CATGGTCCCTTCTTTTGTTTCT
PRIMER_RIGHT_3_SEQUENCE=GCTGCAATTTACCACCAATACA
PRIMER_LEFT_3=181,22
PRIMER_RIGHT_3=445,22
PRIMER_LEFT_3_TM=59.499
PRIMER_RIGHT_3_TM=59.896
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=265
PRIMER_PAIR_PENALTY_4=0.7535
PRIMER_LEFT_4_PENALTY=0.149516
PRIMER_RIGHT_4_PENALTY=0.603960
PRIMER_LEFT_4_SEQUENCE=CTTTGTTTTCTCCAAATCCCAC
PRIMER_RIGHT_4_SEQUENCE=GGCTGCAATTTACCACCAATAC
PRIMER_LEFT_4=201,22
PRIMER_RIGHT_4=446,22
PRIMER_LEFT_4_TM=59.850
PRIMER_RIGHT_4_TM=60.604
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=246
=
PRIMER_SEQUENCE_ID=SSR_06314 Ps_contig_mira-and-tgicl-ass_franssen_43771
SEQUENCE=TGGTAGTTCAACGCAGAGTACGCGGGGATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCAACCCCCAAAACATGGAAACAACCCTCCACTCTATCTTTCATCTCTCTCAAACCAACCAAGTTCACACCAAGAACAACAAAAATTGCTGCAACAGAAGACAAAACCGATGCAGCAACCGATGTAGCAACAAAAGAAGCACCAGTTGGATTCACCCCACCTGAGCTAGACCCAAACACACCTTCTCCAATTTTCG
TARGET=41,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3994
PRIMER_LEFT_PENALTY=0.359545
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=TGGTAGTTCAACGCAGAGTACG
PRIMER_RIGHT_SEQUENCE=ATCCAACTGGTGCTTCTTTTGT
PRIMER_LEFT=0,22
PRIMER_RIGHT=248,22
PRIMER_LEFT_TM=60.360
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.1000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=249
PRIMER_PAIR_PENALTY_1=0.4956
PRIMER_LEFT_1_PENALTY=0.359545
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=TGGTAGTTCAACGCAGAGTACG
PRIMER_RIGHT_1_SEQUENCE=ATAGAGTGGAGGGTTGTTTCCA
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=134,22
PRIMER_LEFT_1_TM=60.360
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=7.1000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=135
PRIMER_PAIR_PENALTY_2=0.4956
PRIMER_LEFT_2_PENALTY=0.359545
PRIMER_RIGHT_2_PENALTY=0.136017
PRIMER_LEFT_2_SEQUENCE=TGGTAGTTCAACGCAGAGTACG
PRIMER_RIGHT_2_SEQUENCE=TAGAGTGGAGGGTTGTTTCCAT
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=133,22
PRIMER_LEFT_2_TM=60.360
PRIMER_RIGHT_2_TM=59.864
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.1000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=0.5251
PRIMER_LEFT_3_PENALTY=0.359545
PRIMER_RIGHT_3_PENALTY=0.165541
PRIMER_LEFT_3_SEQUENCE=TGGTAGTTCAACGCAGAGTACG
PRIMER_RIGHT_3_SEQUENCE=TCTTTTGTTGCTACATCGGTTG
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=234,22
PRIMER_LEFT_3_TM=60.360
PRIMER_RIGHT_3_TM=60.166
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.1000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=235
PRIMER_PAIR_PENALTY_4=0.5565
PRIMER_LEFT_4_PENALTY=0.359545
PRIMER_RIGHT_4_PENALTY=0.196906
PRIMER_LEFT_4_SEQUENCE=TGGTAGTTCAACGCAGAGTACG
PRIMER_RIGHT_4_SEQUENCE=GTTGAGAGTGGTGGTGTGAAGA
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=102,22
PRIMER_LEFT_4_TM=60.360
PRIMER_RIGHT_4_TM=60.197
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=7.1000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=103
=
PRIMER_SEQUENCE_ID=SSR_06315 Ps_contig_mira-and-tgicl-ass_franssen_43774
SEQUENCE=GGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGCTTGATGGGGTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGTGTGTGTATATTGCAATGAAATTAGAAAAGTATTTGTTATGTT
TARGET=187,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.9293
PRIMER_LEFT_PENALTY=0.301926
PRIMER_RIGHT_PENALTY=0.627348
PRIMER_LEFT_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_LEFT=76,22
PRIMER_RIGHT=226,22
PRIMER_LEFT_TM=60.302
PRIMER_RIGHT_TM=59.373
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=151
PRIMER_PAIR_PENALTY_1=0.9293
PRIMER_LEFT_1_PENALTY=0.301926
PRIMER_RIGHT_1_PENALTY=0.627348
PRIMER_LEFT_1_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_1_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_LEFT_1=77,22
PRIMER_RIGHT_1=226,22
PRIMER_LEFT_1_TM=60.302
PRIMER_RIGHT_1_TM=59.373
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=150
PRIMER_PAIR_PENALTY_2=1.2164
PRIMER_LEFT_2_PENALTY=0.589035
PRIMER_RIGHT_2_PENALTY=0.627348
PRIMER_LEFT_2_SEQUENCE=GAGGAAGCTACTTTCTTGGTGG
PRIMER_RIGHT_2_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_LEFT_2=63,22
PRIMER_RIGHT_2=226,22
PRIMER_LEFT_2_TM=59.411
PRIMER_RIGHT_2_TM=59.373
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=8.1000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=164
PRIMER_PAIR_PENALTY_3=1.2881
PRIMER_LEFT_3_PENALTY=0.660713
PRIMER_RIGHT_3_PENALTY=0.627348
PRIMER_LEFT_3_SEQUENCE=TTCTTGGTGGTAGCAGACTTCC
PRIMER_RIGHT_3_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=226,22
PRIMER_LEFT_3_TM=60.661
PRIMER_RIGHT_3_TM=59.373
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=1.6661
PRIMER_LEFT_4_PENALTY=1.038744
PRIMER_RIGHT_4_PENALTY=0.627348
PRIMER_LEFT_4_SEQUENCE=GCTACTTTCTTGGTGGTAGCAGA
PRIMER_RIGHT_4_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_LEFT_4=69,23
PRIMER_RIGHT_4=226,22
PRIMER_LEFT_4_TM=59.961
PRIMER_RIGHT_4_TM=59.373
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=158
=
PRIMER_SEQUENCE_ID=SSR_06316 Ps_contig_mira-and-tgicl-ass_franssen_43789
SEQUENCE=GTCGGGGTACTAACTAACTATTTGTCTAGTAGCATCGAAGAGAACAACATCAAAGTGCAAGGAGTCTCAACGCCCTCATGGCCACCTCTGCTATCCAACAATCAGCTTTCACTGGTAAGACTGGTTTGAGGCAGGGAAATGAATTCATCAGGAAAGCTGGAAACTTTGGCCAAGCTCGCTTTACCATGCGTCGTACTGTGAAGAGTGCTCCTGAAAGCTATTTGGTA
TARGET=9,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06317 Ps_contig_mira-and-tgicl-ass_franssen_43875
SEQUENCE=AAGCAGTGTATCAACGCAGAGTAYGCGGGGGATCTTCAAGCAATCTTTCGCCTTTACACCATCTCAGTCCCAATTTCAGTGTTTCTTCCATGGCTTCCTCAGATGTTGAGTACCGGTGCTTCGTTGGTGGTCTAGCATGGGCCACTGACAGCGATGCCCTAGAGAAAGCTTTCTCTCAATACGGTGAAATCGTCGACTCGAAGATCATTAACGATCGTGAGACTGGAAGGTCAAGAGGTTTTGGATTCGTGACCTTTGCCACGGAGCAATCCATGAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAATTGAAAAAATAGTGAGATATTAGTGGTTACAGATTTTGATAATGAATTCGGTTTTCTTTTACCTTCGACCANNNTTTCGCATTATCTATCCCATCATATATTCATGGATTAAGGTTAGGTAACTAACCTGAGTATTCTTGAGTACTT
TARGET=400,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06318 Ps_contig_mira-and-tgicl-ass_franssen_43875
SEQUENCE=AAGCAGTGTATCAACGCAGAGTAYGCGGGGGATCTTCAAGCAATCTTTCGCCTTTACACCATCTCAGTCCCAATTTCAGTGTTTCTTCCATGGCTTCCTCAGATGTTGAGTACCGGTGCTTCGTTGGTGGTCTAGCATGGGCCACTGACAGCGATGCCCTAGAGAAAGCTTTCTCTCAATACGGTGAAATCGTCGACTCGAAGATCATTAACGATCGTGAGACTGGAAGGTCAAGAGGTTTTGGATTCGTGACCTTTGCCACGGAGCAATCCATGAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAATTGAAAAAATAGTGAGATATTAGTGGTTACAGATTTTGATAATGAATTCGGTTTTCTTTTACCTTCGACCANNNTTTCGCATTATCTATCCCATCATATATTCATGGATTAAGGTTAGGTAACTAACCTGAGTATTCTTGAGTACTT
TARGET=453,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06319 Ps_contig_mira-and-tgicl-ass_franssen_43875
SEQUENCE=AAGCAGTGTATCAACGCAGAGTAYGCGGGGGATCTTCAAGCAATCTTTCGCCTTTACACCATCTCAGTCCCAATTTCAGTGTTTCTTCCATGGCTTCCTCAGATGTTGAGTACCGGTGCTTCGTTGGTGGTCTAGCATGGGCCACTGACAGCGATGCCCTAGAGAAAGCTTTCTCTCAATACGGTGAAATCGTCGACTCGAAGATCATTAACGATCGTGAGACTGGAAGGTCAAGAGGTTTTGGATTCGTGACCTTTGCCACGGAGCAATCCATGAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAATTGAAAAAATAGTGAGATATTAGTGGTTACAGATTTTGATAATGAATTCGGTTTTCTTTTACCTTCGACCANNNTTTCGCATTATCTATCCCATCATATATTCATGGATTAAGGTTAGGTAACTAACCTGAGTATTCTTGAGTACTT
TARGET=474,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06320 Ps_contig_mira-and-tgicl-ass_franssen_43875
SEQUENCE=AAGCAGTGTATCAACGCAGAGTAYGCGGGGGATCTTCAAGCAATCTTTCGCCTTTACACCATCTCAGTCCCAATTTCAGTGTTTCTTCCATGGCTTCCTCAGATGTTGAGTACCGGTGCTTCGTTGGTGGTCTAGCATGGGCCACTGACAGCGATGCCCTAGAGAAAGCTTTCTCTCAATACGGTGAAATCGTCGACTCGAAGATCATTAACGATCGTGAGACTGGAAGGTCAAGAGGTTTTGGATTCGTGACCTTTGCCACGGAGCAATCCATGAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAATTGAAAAAATAGTGAGATATTAGTGGTTACAGATTTTGATAATGAATTCGGTTTTCTTTTACCTTCGACCANNNTTTCGCATTATCTATCCCATCATATATTCATGGATTAAGGTTAGGTAACTAACCTGAGTATTCTTGAGTACTT
TARGET=488,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06321 Ps_contig_mira-and-tgicl-ass_franssen_43875
SEQUENCE=AAGCAGTGTATCAACGCAGAGTAYGCGGGGGATCTTCAAGCAATCTTTCGCCTTTACACCATCTCAGTCCCAATTTCAGTGTTTCTTCCATGGCTTCCTCAGATGTTGAGTACCGGTGCTTCGTTGGTGGTCTAGCATGGGCCACTGACAGCGATGCCCTAGAGAAAGCTTTCTCTCAATACGGTGAAATCGTCGACTCGAAGATCATTAACGATCGTGAGACTGGAAGGTCAAGAGGTTTTGGATTCGTGACCTTTGCCACGGAGCAATCCATGAGAGATGCGATCGAAGGGATGAACGGTCAGGACATGGATGGACGTAACATCACAGTAAACGAAGCTCAGAGCCGTGGTAGCGGTGGAGGCGGCCGTGGTGGTGGCGGTGGATATGGAGGTGGACGTGGTGGTGGTGGATATGGTGGAGGTGGAGGCTATGGTGGCCGTCGTGAAGGTGGAGGAGGAGGATACGGTAGTGGAGGAGGAGGATATGGTGGTGGTGGTTATGGCGGAAGCAGGGATCGTGGTTATGGAGGAAGTGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGTGCATAACGATCAATTGAAAAAATAGTGAGATATTAGTGGTTACAGATTTTGATAATGAATTCGGTTTTCTTTTACCTTCGACCANNNTTTCGCATTATCTATCCCATCATATATTCATGGATTAAGGTTAGGTAACTAACCTGAGTATTCTTGAGTACTT
TARGET=564,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06322 Ps_contig_mira-and-tgicl-ass_franssen_43917
SEQUENCE=AACGGAAGGTTTAGGTTTTGGGGTAAAGGGTTTGGTAAAAGGGGAATTAGGGTGGTTTGTCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTCTTTTTTTACTTGTGTACACTTCTTACCAGAAAGAATATGGTTGTTGTTTACTTTGTAACAATTTTCTTATCATCTTTTGTCTTGTAATTAGTTAACTACCAAAAGTAACTTTA
TARGET=190,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06323 Ps_contig_mira-and-tgicl-ass_franssen_43949
SEQUENCE=ATATTCGTCATTTCATTCCCATTCTTTTTCTACAGAACCTCGAGAGCAAGAAGAGTGGGGAGCATAGACAGTGACAGAGACTGTGTTTGTGAAAGAGAGAGAGATTTCGCAATTCGCAAACCCTAATCAAAGAGATCGGCGATGGCTTCCACCAAGGATCGCGAGAACTTCGTCTACATCGCAAAGCTCGCTGAGCAAGCTGAACGATACGAAGAGATGGTGGATTCGATGAAGAATGTTGCAAATCTAGATGTTGAACTGACTATTGAAGAAAGGAATTTGCTTTCTGTTGGTTATAAGAACGTGATTGGTGCTCGCAGAGCGTCGTGGAGGATTTTGTCTTCCATTGAGCAAAAGGAAGAGTCAAAGGGAAATGATGTAAATGCGAAACGGATTAAGGAGTATAGGCATAAGGTTGAAACTGAGCTTTCAAACATCTGTATTGATGTTATGAGAGTGATTGATGAACACCTTATACCTTCAGCTGCAGCGGGTGAATCAACTGTGTTTTACTATAAGATGAAAGGAGATTATTATCGTTACCTTGCGGAATTTAAGACAGGCAATGAGAAGAAGGAGGCTGGTGATCAGTCGATGAAGGCATACGAGTCTGCTACCACTGCAGCAGAGGCTGAATTACCCCCTACTCATCCCATTCGATTGGGTCTGGCTTTGAATTTCTCAGTTTTCTATTATGAGATCTTGAATTCACCAGAAAGAGCCTGCCATCTTGCAAAGCAGGCATTTGATGAAGCTATTTCAGAGCTTGACACCTTGAATGAGGAGTCCTACAAAGATAGTACATTGATCATGCAACTTCTCAGGGACAACCTGACTCTCTGGACTTCTGACATCCCCGAAGATGGAGAAGATTCCCAGAAAGCGAATGGCACTGCCAAGTTTGGTGGAGGTGATGATGCAGAGTGAGACTGGTGTTTGGTGGGAACTTCCTTAGGATCTGATGCAGGTGTTTGAGTATGAAACATTAGGGCTGATTACTTATTTGTGTACTAGCTAGAGTTAGGGTTCCATTGTTTCTCTTTCTCTGTTTCTTGTCAATTGGCCATACTTTCCCATAGAGGAAAACGAAACTCCATTTGTGCTATATTATGATTATGTTTTTATTTGTGGTGTGTCTTGAAATGGCCTGTTTCACTCCTCAGCACCTCCTTCTGTCTTTGGGAGATTGATTTTATGAGGATTGTTCTTGCTGTATTTCCAGTTCCAATGAAATGCTTCACCTTTATATGGAGATTGATCACTTGATTATTATTCTAAACTTAAGAAAAACTT
TARGET=94,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3205
PRIMER_LEFT_PENALTY=0.188254
PRIMER_RIGHT_PENALTY=0.132262
PRIMER_LEFT_SEQUENCE=ACAGAACCTCGAGAGCAAGAAG
PRIMER_RIGHT_SEQUENCE=CACCATCTCTTCGTATCGTTCA
PRIMER_LEFT=31,22
PRIMER_RIGHT=221,22
PRIMER_LEFT_TM=60.188
PRIMER_RIGHT_TM=60.132
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=191
PRIMER_PAIR_PENALTY_1=0.3205
PRIMER_LEFT_1_PENALTY=0.188254
PRIMER_RIGHT_1_PENALTY=0.132262
PRIMER_LEFT_1_SEQUENCE=AACCTCGAGAGCAAGAAGAGTG
PRIMER_RIGHT_1_SEQUENCE=CACCATCTCTTCGTATCGTTCA
PRIMER_LEFT_1=35,22
PRIMER_RIGHT_1=221,22
PRIMER_LEFT_1_TM=60.188
PRIMER_RIGHT_1_TM=60.132
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=187
PRIMER_PAIR_PENALTY_2=0.3336
PRIMER_LEFT_2_PENALTY=0.188254
PRIMER_RIGHT_2_PENALTY=0.145354
PRIMER_LEFT_2_SEQUENCE=ACAGAACCTCGAGAGCAAGAAG
PRIMER_RIGHT_2_SEQUENCE=ATCCACCATCTCTTCGTATCGT
PRIMER_LEFT_2=31,22
PRIMER_RIGHT_2=224,22
PRIMER_LEFT_2_TM=60.188
PRIMER_RIGHT_2_TM=59.855
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=8.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=194
PRIMER_PAIR_PENALTY_3=0.3479
PRIMER_LEFT_3_PENALTY=0.188254
PRIMER_RIGHT_3_PENALTY=0.159665
PRIMER_LEFT_3_SEQUENCE=ACAGAACCTCGAGAGCAAGAAG
PRIMER_RIGHT_3_SEQUENCE=ATTCTTCATCGAATCCACCATC
PRIMER_LEFT_3=31,22
PRIMER_RIGHT_3=236,22
PRIMER_LEFT_3_TM=60.188
PRIMER_RIGHT_3_TM=60.160
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.1000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=206
PRIMER_PAIR_PENALTY_4=0.3479
PRIMER_LEFT_4_PENALTY=0.188254
PRIMER_RIGHT_4_PENALTY=0.159665
PRIMER_LEFT_4_SEQUENCE=AACCTCGAGAGCAAGAAGAGTG
PRIMER_RIGHT_4_SEQUENCE=ATTCTTCATCGAATCCACCATC
PRIMER_LEFT_4=35,22
PRIMER_RIGHT_4=236,22
PRIMER_LEFT_4_TM=60.188
PRIMER_RIGHT_4_TM=60.160
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=202
=
PRIMER_SEQUENCE_ID=SSR_06324 Ps_contig_mira-and-tgicl-ass_franssen_43968
SEQUENCE=AGGGACATAACCAAAAATACACTCATAAGAAAAACAATGAGGTCCCTAATGGCTTCTGCTTCTCTCACTCTTGCATTAGCCATACTTCTTTTTCAGTTTCCCATTCTAGAAATATCAGNNAAACCAATTATTTCTTTACTTCCTCNACCACCACCACCGGTCCACTCCACCCACCGCCAACCAAAGGNTCCATACC
TARGET=147,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3736
PRIMER_LEFT_PENALTY=0.154023
PRIMER_RIGHT_PENALTY=2.219551
PRIMER_LEFT_SEQUENCE=TCTCTCACTCTTGCATTAGCCA
PRIMER_RIGHT_SEQUENCE=GGTATGGANCCTTTGGTTGG
PRIMER_LEFT=60,22
PRIMER_RIGHT=195,20
PRIMER_LEFT_TM=60.154
PRIMER_RIGHT_TM=60.220
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=52.632
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.50
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=136
PRIMER_PAIR_PENALTY_1=2.3802
PRIMER_LEFT_1_PENALTY=0.160609
PRIMER_RIGHT_1_PENALTY=2.219551
PRIMER_LEFT_1_SEQUENCE=ATGGCTTCTGCTTCTCTCACTC
PRIMER_RIGHT_1_SEQUENCE=GGTATGGANCCTTTGGTTGG
PRIMER_LEFT_1=48,22
PRIMER_RIGHT_1=195,20
PRIMER_LEFT_1_TM=60.161
PRIMER_RIGHT_1_TM=60.220
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=52.632
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.50
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=2.75
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=148
PRIMER_PAIR_PENALTY_2=2.5258
PRIMER_LEFT_2_PENALTY=0.160609
PRIMER_RIGHT_2_PENALTY=2.365170
PRIMER_LEFT_2_SEQUENCE=ATGGCTTCTGCTTCTCTCACTC
PRIMER_RIGHT_2_SEQUENCE=GTATGGANCCTTTGGTTGGC
PRIMER_LEFT_2=48,22
PRIMER_RIGHT_2=194,20
PRIMER_LEFT_2_TM=60.161
PRIMER_RIGHT_2_TM=60.365
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=52.632
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.50
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=10.0000
PRIMER_PAIR_2_COMPL_ANY=2.75
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=147
PRIMER_PAIR_PENALTY_3=2.5484
PRIMER_LEFT_3_PENALTY=0.328860
PRIMER_RIGHT_3_PENALTY=2.219551
PRIMER_LEFT_3_SEQUENCE=AATGAGGTCCCTAATGGCTTCT
PRIMER_RIGHT_3_SEQUENCE=GGTATGGANCCTTTGGTTGG
PRIMER_LEFT_3=35,22
PRIMER_RIGHT_3=195,20
PRIMER_LEFT_3_TM=60.329
PRIMER_RIGHT_3_TM=60.220
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=52.632
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.50
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=161
PRIMER_PAIR_PENALTY_4=2.5539
PRIMER_LEFT_4_PENALTY=0.334369
PRIMER_RIGHT_4_PENALTY=2.219551
PRIMER_LEFT_4_SEQUENCE=AATGGCTTCTGCTTCTCTCACT
PRIMER_RIGHT_4_SEQUENCE=GGTATGGANCCTTTGGTTGG
PRIMER_LEFT_4=47,22
PRIMER_RIGHT_4=195,20
PRIMER_LEFT_4_TM=59.666
PRIMER_RIGHT_4_TM=60.220
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=52.632
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.50
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.75
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=149
=
PRIMER_SEQUENCE_ID=SSR_06325 Ps_contig_mira-and-tgicl-ass_franssen_43998
SEQUENCE=AGGGATCGTGGTTATGGAGGAAGTGGTNGGGGGTTAGGGTTGGNTGGTTATTCTCGGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAATCTAGATCTAGAATTGGGTACTTGTTCTGTAATGGACCTGTTGTTCTTTACTGGATTCTTGGTTTTCTGTGTTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGTCGTTGGGTTGTGACTAATAACGATACTAAATTAAGAAAAAATGTGAGTATNNAGTAATTTTAA
TARGET=62,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3921
PRIMER_LEFT_PENALTY=0.354011
PRIMER_RIGHT_PENALTY=0.038090
PRIMER_LEFT_SEQUENCE=GGTTAGGGTTGGNTGGTTATTC
PRIMER_RIGHT_SEQUENCE=ACAACCCAACGACTTCAGAGAT
PRIMER_LEFT=31,22
PRIMER_RIGHT=286,22
PRIMER_LEFT_TM=59.646
PRIMER_RIGHT_TM=60.038
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.9000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=7.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=256
PRIMER_PAIR_PENALTY_1=0.7811
PRIMER_LEFT_1_PENALTY=0.742980
PRIMER_RIGHT_1_PENALTY=0.038090
PRIMER_LEFT_1_SEQUENCE=GATCGTGGTTATGGAGGAAGTG
PRIMER_RIGHT_1_SEQUENCE=ACAACCCAACGACTTCAGAGAT
PRIMER_LEFT_1=3,22
PRIMER_RIGHT_1=286,22
PRIMER_LEFT_1_TM=60.743
PRIMER_RIGHT_1_TM=60.038
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.3000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=284
PRIMER_PAIR_PENALTY_2=0.9369
PRIMER_LEFT_2_PENALTY=0.354011
PRIMER_RIGHT_2_PENALTY=0.582904
PRIMER_LEFT_2_SEQUENCE=GGTTAGGGTTGGNTGGTTATTC
PRIMER_RIGHT_2_SEQUENCE=TAGTCACAACCCAACGACTTCA
PRIMER_LEFT_2=31,22
PRIMER_RIGHT_2=291,22
PRIMER_LEFT_2_TM=59.646
PRIMER_RIGHT_2_TM=60.583
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.75
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=1.0508
PRIMER_LEFT_3_PENALTY=1.012719
PRIMER_RIGHT_3_PENALTY=0.038090
PRIMER_LEFT_3_SEQUENCE=GGGTTAGGGTTGGNTGGTTAT
PRIMER_RIGHT_3_SEQUENCE=ACAACCCAACGACTTCAGAGAT
PRIMER_LEFT_3=30,21
PRIMER_RIGHT_3=286,22
PRIMER_LEFT_3_TM=59.987
PRIMER_RIGHT_3_TM=60.038
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.3000
PRIMER_PAIR_3_COMPL_ANY=7.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=257
PRIMER_PAIR_PENALTY_4=1.1157
PRIMER_LEFT_4_PENALTY=0.354011
PRIMER_RIGHT_4_PENALTY=0.761640
PRIMER_LEFT_4_SEQUENCE=GGTTAGGGTTGGNTGGTTATTC
PRIMER_RIGHT_4_SEQUENCE=CAACCCAACGACTTCAGAGATA
PRIMER_LEFT_4=31,22
PRIMER_RIGHT_4=285,22
PRIMER_LEFT_4_TM=59.646
PRIMER_RIGHT_4_TM=59.238
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=5.9000
PRIMER_RIGHT_4_END_STABILITY=5.6000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=255
=
PRIMER_SEQUENCE_ID=SSR_06326 Ps_contig_mira-and-tgicl-ass_franssen_44013
SEQUENCE=CTGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGTATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGCGAGTTCTTTTTTTACTGTGTACACTTCTAA
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=4.4060
PRIMER_LEFT_PENALTY=0.371199
PRIMER_RIGHT_PENALTY=4.034774
PRIMER_LEFT_SEQUENCE=ATACGTTCTTCAATGGCGAAAC
PRIMER_RIGHT_SEQUENCE=AACTCGCAACACATAAGAGATACTG
PRIMER_LEFT=85,22
PRIMER_RIGHT=215,25
PRIMER_LEFT_TM=60.371
PRIMER_RIGHT_TM=58.965
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=131
PRIMER_PAIR_PENALTY_1=4.4274
PRIMER_LEFT_1_PENALTY=0.392624
PRIMER_RIGHT_1_PENALTY=4.034774
PRIMER_LEFT_1_SEQUENCE=AGTTGAAAGGATTGGTGTTTGC
PRIMER_RIGHT_1_SEQUENCE=AACTCGCAACACATAAGAGATACTG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=215,25
PRIMER_LEFT_1_TM=60.393
PRIMER_RIGHT_1_TM=58.965
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.000
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=5.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=208
PRIMER_PAIR_PENALTY_2=4.5060
PRIMER_LEFT_2_PENALTY=0.471231
PRIMER_RIGHT_2_PENALTY=4.034774
PRIMER_LEFT_2_SEQUENCE=CATCAAGCAGGATCGGATAGTT
PRIMER_RIGHT_2_SEQUENCE=AACTCGCAACACATAAGAGATACTG
PRIMER_LEFT_2=30,22
PRIMER_RIGHT_2=215,25
PRIMER_LEFT_2_TM=60.471
PRIMER_RIGHT_2_TM=58.965
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=186
PRIMER_PAIR_PENALTY_3=4.6640
PRIMER_LEFT_3_PENALTY=0.629225
PRIMER_RIGHT_3_PENALTY=4.034774
PRIMER_LEFT_3_SEQUENCE=GGAAGTTGAAAGGATTGGTGTT
PRIMER_RIGHT_3_SEQUENCE=AACTCGCAACACATAAGAGATACTG
PRIMER_LEFT_3=5,22
PRIMER_RIGHT_3=215,25
PRIMER_LEFT_3_TM=59.371
PRIMER_RIGHT_3_TM=58.965
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=5.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=211
PRIMER_PAIR_PENALTY_4=4.6640
PRIMER_LEFT_4_PENALTY=0.629225
PRIMER_RIGHT_4_PENALTY=4.034774
PRIMER_LEFT_4_SEQUENCE=GTTGGAAGTTGAAAGGATTGGT
PRIMER_RIGHT_4_SEQUENCE=AACTCGCAACACATAAGAGATACTG
PRIMER_LEFT_4=2,22
PRIMER_RIGHT_4=215,25
PRIMER_LEFT_4_TM=59.371
PRIMER_RIGHT_4_TM=58.965
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.000
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_06327 Ps_contig_mira-and-tgicl-ass_franssen_44042
SEQUENCE=AGGAGATATANATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGYGGAGATGGANRNTTANATGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAACCTAGATCTAGAATTGGGTACYTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGT
TARGET=19,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06328 Ps_contig_mira-and-tgicl-ass_franssen_44042
SEQUENCE=AGGAGATATANATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGYGGAGATGGANRNTTANATGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAACCTAGATCTAGAATTGGGTACYTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGT
TARGET=50,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06329 Ps_contig_mira-and-tgicl-ass_franssen_44042
SEQUENCE=AGGAGATATANATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGYGGAGATGGANRNTTANATGGTGGAGGAGGTGGTTATTCTCGTGGAGGCGGCGGCGGCGGTGGAAACTGGAGGGAGTAGTGTTTTAGATGGTTATCGTTTATGATTATTTAAGTTTAATTCTAGATGTTGAGATCTACTGATTGATCAACCTAGATCTAGAATTGGGTACYTGTTCTGTAATGGATCTGTTGTTCTTTACTGGATCTGGTTTTCTGTGTCTTGTTTGTGTGTCTCTGAATCAAGTGTATCTCTGAAGCTGGTTGT
TARGET=134,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06330 Ps_contig_mira-and-tgicl-ass_franssen_44061
SEQUENCE=TAACCTTGTTGATGTTTTGGCTTGGGGTTCTATAGGTCATATTGTTGCTTATTATATCTTGGCCACTTCTAGCAATGGTTATGATCCTAGTTTCTTTGGTTAATTAAAGATTACTATGTTGTTCGTTTATGTTGTTTTAATTTGTCTTTTCTTGTCCCTTTTATGTTTATTAAGATGATGATGATGATGATGAATGATGTATAGTTTATTTGTATGAGATATTACAAACCAAAGTTAAGTTCCTTAGGAAAAAATT
TARGET=174,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06331 Ps_contig_mira-and-tgicl-ass_franssen_44118
SEQUENCE=GNTCGGGGACAATCATCATATCCATAGACCCTTCTTTATTACTATTGTTACCAATATCAGCATCATCATCATCATCACATTCAAGTTCAAGAAAGAAAGAAACTACTATGGCTCAAGCTATGGCATCATCAATGGCTGGTTGTTTACGTGGTTGTTCATCTCAAACTGTGTTAGAAGGAAGTCTTCAATTTAGTGGACCAAACAGGTTGAGTTTGTTGCACGGAAACACCAACAACGTTAACAAGGTTACAACACGTTCATCAGTGACGGTTAGAGCTCAACAACAGGAGAGTAGTCGAAGGGCTGTGA
TARGET=61,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5056
PRIMER_LEFT_PENALTY=1.443625
PRIMER_RIGHT_PENALTY=0.061961
PRIMER_LEFT_SEQUENCE=GNTCGGGGACAATCATCATATC
PRIMER_RIGHT_SEQUENCE=AACTCAACCTGTTTGGTCCACT
PRIMER_LEFT=0,22
PRIMER_RIGHT=212,22
PRIMER_LEFT_TM=61.444
PRIMER_RIGHT_TM=59.938
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=5.5000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=213
PRIMER_PAIR_PENALTY_1=1.6370
PRIMER_LEFT_1_PENALTY=1.443625
PRIMER_RIGHT_1_PENALTY=0.193337
PRIMER_LEFT_1_SEQUENCE=GNTCGGGGACAATCATCATATC
PRIMER_RIGHT_1_SEQUENCE=TGTTAACGTTGTTGGTGTTTCC
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=242,22
PRIMER_LEFT_1_TM=61.444
PRIMER_RIGHT_1_TM=59.807
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=5.5000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=243
PRIMER_PAIR_PENALTY_2=1.6465
PRIMER_LEFT_2_PENALTY=1.584586
PRIMER_RIGHT_2_PENALTY=0.061961
PRIMER_LEFT_2_SEQUENCE=GGGGACAATCATCATATCCATAG
PRIMER_RIGHT_2_SEQUENCE=AACTCAACCTGTTTGGTCCACT
PRIMER_LEFT_2=4,23
PRIMER_RIGHT_2=212,22
PRIMER_LEFT_2_TM=59.415
PRIMER_RIGHT_2_TM=59.938
PRIMER_LEFT_2_GC_PERCENT=43.478
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=209
PRIMER_PAIR_PENALTY_3=1.6469
PRIMER_LEFT_3_PENALTY=1.443625
PRIMER_RIGHT_3_PENALTY=0.203324
PRIMER_LEFT_3_SEQUENCE=GNTCGGGGACAATCATCATATC
PRIMER_RIGHT_3_SEQUENCE=CTCTAACCGTCACTGATGAACG
PRIMER_LEFT_3=0,22
PRIMER_RIGHT_3=275,22
PRIMER_LEFT_3_TM=61.444
PRIMER_RIGHT_3_TM=59.797
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.5000
PRIMER_RIGHT_3_END_STABILITY=8.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=276
PRIMER_PAIR_PENALTY_4=1.7456
PRIMER_LEFT_4_PENALTY=1.443625
PRIMER_RIGHT_4_PENALTY=0.301926
PRIMER_LEFT_4_SEQUENCE=GNTCGGGGACAATCATCATATC
PRIMER_RIGHT_4_SEQUENCE=AAACTCAACCTGTTTGGTCCAC
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=213,22
PRIMER_LEFT_4_TM=61.444
PRIMER_RIGHT_4_TM=60.302
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.5000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_06332 Ps_contig_mira-and-tgicl-ass_franssen_44118
SEQUENCE=GNTCGGGGACAATCATCATATCCATAGACCCTTCTTTATTACTATTGTTACCAATATCAGCATCATCATCATCATCACATTCAAGTTCAAGAAAGAAAGAAACTACTATGGCTCAAGCTATGGCATCATCAATGGCTGGTTGTTTACGTGGTTGTTCATCTCAAACTGTGTTAGAAGGAAGTCTTCAATTTAGTGGACCAAACAGGTTGAGTTTGTTGCACGGAAACACCAACAACGTTAACAAGGTTACAACACGTTCATCAGTGACGGTTAGAGCTCAACAACAGGAGAGTAGTCGAAGGGCTGTGA
TARGET=89,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5748
PRIMER_LEFT_PENALTY=0.512874
PRIMER_RIGHT_PENALTY=0.061961
PRIMER_LEFT_SEQUENCE=TCAGCATCATCATCATCATCAC
PRIMER_RIGHT_SEQUENCE=AACTCAACCTGTTTGGTCCACT
PRIMER_LEFT=56,22
PRIMER_RIGHT=212,22
PRIMER_LEFT_TM=59.487
PRIMER_RIGHT_TM=59.938
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.3000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=157
PRIMER_PAIR_PENALTY_1=0.5754
PRIMER_LEFT_1_PENALTY=0.513452
PRIMER_RIGHT_1_PENALTY=0.061961
PRIMER_LEFT_1_SEQUENCE=CAGCATCATCATCATCATCACA
PRIMER_RIGHT_1_SEQUENCE=AACTCAACCTGTTTGGTCCACT
PRIMER_LEFT_1=57,22
PRIMER_RIGHT_1=212,22
PRIMER_LEFT_1_TM=60.513
PRIMER_RIGHT_1_TM=59.938
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=156
PRIMER_PAIR_PENALTY_2=0.7062
PRIMER_LEFT_2_PENALTY=0.512874
PRIMER_RIGHT_2_PENALTY=0.193337
PRIMER_LEFT_2_SEQUENCE=TCAGCATCATCATCATCATCAC
PRIMER_RIGHT_2_SEQUENCE=TGTTAACGTTGTTGGTGTTTCC
PRIMER_LEFT_2=56,22
PRIMER_RIGHT_2=242,22
PRIMER_LEFT_2_TM=59.487
PRIMER_RIGHT_2_TM=59.807
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.3000
PRIMER_RIGHT_2_END_STABILITY=8.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=187
PRIMER_PAIR_PENALTY_3=0.7068
PRIMER_LEFT_3_PENALTY=0.513452
PRIMER_RIGHT_3_PENALTY=0.193337
PRIMER_LEFT_3_SEQUENCE=CAGCATCATCATCATCATCACA
PRIMER_RIGHT_3_SEQUENCE=TGTTAACGTTGTTGGTGTTTCC
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=242,22
PRIMER_LEFT_3_TM=60.513
PRIMER_RIGHT_3_TM=59.807
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=186
PRIMER_PAIR_PENALTY_4=0.7168
PRIMER_LEFT_4_PENALTY=0.513452
PRIMER_RIGHT_4_PENALTY=0.203324
PRIMER_LEFT_4_SEQUENCE=CAGCATCATCATCATCATCACA
PRIMER_RIGHT_4_SEQUENCE=CTCTAACCGTCACTGATGAACG
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=275,22
PRIMER_LEFT_4_TM=60.513
PRIMER_RIGHT_4_TM=59.797
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.4000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=219
=
PRIMER_SEQUENCE_ID=SSR_06333 Ps_contig_mira-and-tgicl-ass_franssen_44128
SEQUENCE=TTTTTTTTTATTAATANAGGATATATTATTTTGGCTTGCTTGATCAAAACCGAACTTTGAAAATTACAATTCATGCAAACAACTGCAGACACATGCATATTGAGGGCAGTGCCTTACAGCTGTGGCTAATACATCAAAAACACAAATTAAAAAAATAAAAGCAGCCCCGAAACACTATTAATCAAACATTGACGAAATATATATGTGAAGAATCCCCTCGATCTTCTTTGTGCATGGCTTTCTTTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATCGACTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGYNTGCTCTCTCTTTCGTCTCAAGAACAAAATCGCGATTCGCTCTTCTCCGAAAAAACCTCTAGATCGAAAAGAAGAGCGTATTTTGTGTGTTGGTGAAGATGGGGTTAACGTTCACGAAGCTGTTCAGTAGGTTATTCGCGAAGAAGGAAATGAGGATTTTGGTTGGTCTCGATGCTGCTGGTAAGACAACTATTTTGTATAAACTCAAGCTTGGAGAAATCGTCACTACCATCCCAACCATTGGATTTAATGTTGAAACCGTGGAGTACAAGAACATCAGCTTCACCGTGTGGGATGTCGGGGTCAGGACAAGATCCGCCCATTGTGGAGGCACTACTTCCAGAACACTCAGGGTCTTATTTTGTGGTTGATAGCAATGRTAGAGATCGAGTGGTTGAGGCAAGGGATGAGTTGCACAGGATGTTGAATGAGGACGAACTTAGAGATGCTTGTTTTGTTTGTTTTGCCAAACAAACAAGATCCTTCCCAATGCAATGAATGCTGCAAGAAAAGTAACGGAACTAAACTTGGTCTTCATTCACCTCCGTACAACCGCCACGTGGTATA
TARGET=363,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06334 Ps_contig_mira-and-tgicl-ass_franssen_44128
SEQUENCE=TTTTTTTTTATTAATANAGGATATATTATTTTGGCTTGCTTGATCAAAACCGAACTTTGAAAATTACAATTCATGCAAACAACTGCAGACACATGCATATTGAGGGCAGTGCCTTACAGCTGTGGCTAATACATCAAAAACACAAATTAAAAAAATAAAAGCAGCCCCGAAACACTATTAATCAAACATTGACGAAATATATATGTGAAGAATCCCCTCGATCTTCTTTGTGCATGGCTTTCTTTGCTTTCACACTTTCTGCTTAGATCCATATAGTGTGATGCCAATGATCGACTATCTAGTGGTGGTAAGGTGGAGGAGGTGATTTGTAGTAATAGGCTGGTGGAGGTGGGGAGTAAACTGGAGGAGGAGGAGAATGGTAAACTGGGGTGGGAACATGAGGAGGGTAGGTGTGAGCTGGTGGTGGTGGTGGTGATGGATACTTGYNTGCTCTCTCTTTCGTCTCAAGAACAAAATCGCGATTCGCTCTTCTCCGAAAAAACCTCTAGATCGAAAAGAAGAGCGTATTTTGTGTGTTGGTGAAGATGGGGTTAACGTTCACGAAGCTGTTCAGTAGGTTATTCGCGAAGAAGGAAATGAGGATTTTGGTTGGTCTCGATGCTGCTGGTAAGACAACTATTTTGTATAAACTCAAGCTTGGAGAAATCGTCACTACCATCCCAACCATTGGATTTAATGTTGAAACCGTGGAGTACAAGAACATCAGCTTCACCGTGTGGGATGTCGGGGTCAGGACAAGATCCGCCCATTGTGGAGGCACTACTTCCAGAACACTCAGGGTCTTATTTTGTGGTTGATAGCAATGRTAGAGATCGAGTGGTTGAGGCAAGGGATGAGTTGCACAGGATGTTGAATGAGGACGAACTTAGAGATGCTTGTTTTGTTTGTTTTGCCAAACAAACAAGATCCTTCCCAATGCAATGAATGCTGCAAGAAAAGTAACGGAACTAAACTTGGTCTTCATTCACCTCCGTACAACCGCCACGTGGTATA
TARGET=419,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06335 Ps_contig_mira-and-tgicl-ass_franssen_44136
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTTTTACTGTGTACACTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06336 Ps_contig_mira-and-tgicl-ass_franssen_44177
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTNTAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTTGTGTACACTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06337 Ps_contig_mira-and-tgicl-ass_franssen_44208
SEQUENCE=CCAGTCAAGTAGGATGGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCAGGGGCTTCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGCTTGATGGGGTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGTGTGTGTATATTGA
TARGET=202,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.9542
PRIMER_LEFT_PENALTY=0.301926
PRIMER_RIGHT_PENALTY=1.652290
PRIMER_LEFT_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_SEQUENCE=AATATACACACACGATGGCCG
PRIMER_LEFT=91,22
PRIMER_RIGHT=237,21
PRIMER_LEFT_TM=60.302
PRIMER_RIGHT_TM=60.652
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=47.619
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=147
PRIMER_PAIR_PENALTY_1=1.9542
PRIMER_LEFT_1_PENALTY=0.301926
PRIMER_RIGHT_1_PENALTY=1.652290
PRIMER_LEFT_1_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_1_SEQUENCE=AATATACACACACGATGGCCG
PRIMER_LEFT_1=92,22
PRIMER_RIGHT_1=237,21
PRIMER_LEFT_1_TM=60.302
PRIMER_RIGHT_1_TM=60.652
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=47.619
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=146
PRIMER_PAIR_PENALTY_2=2.2413
PRIMER_LEFT_2_PENALTY=0.589035
PRIMER_RIGHT_2_PENALTY=1.652290
PRIMER_LEFT_2_SEQUENCE=GAGGAAGCTACTTTCTTGGTGG
PRIMER_RIGHT_2_SEQUENCE=AATATACACACACGATGGCCG
PRIMER_LEFT_2=78,22
PRIMER_RIGHT_2=237,21
PRIMER_LEFT_2_TM=59.411
PRIMER_RIGHT_2_TM=60.652
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=47.619
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=160
PRIMER_PAIR_PENALTY_3=2.3130
PRIMER_LEFT_3_PENALTY=0.660713
PRIMER_RIGHT_3_PENALTY=1.652290
PRIMER_LEFT_3_SEQUENCE=TTCTTGGTGGTAGCAGACTTCC
PRIMER_RIGHT_3_SEQUENCE=AATATACACACACGATGGCCG
PRIMER_LEFT_3=90,22
PRIMER_RIGHT_3=237,21
PRIMER_LEFT_3_TM=60.661
PRIMER_RIGHT_3_TM=60.652
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=47.619
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=12.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=148
PRIMER_PAIR_PENALTY_4=2.6910
PRIMER_LEFT_4_PENALTY=1.038744
PRIMER_RIGHT_4_PENALTY=1.652290
PRIMER_LEFT_4_SEQUENCE=GCTACTTTCTTGGTGGTAGCAGA
PRIMER_RIGHT_4_SEQUENCE=AATATACACACACGATGGCCG
PRIMER_LEFT_4=84,23
PRIMER_RIGHT_4=237,21
PRIMER_LEFT_4_TM=59.961
PRIMER_RIGHT_4_TM=60.652
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=12.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=154
=
PRIMER_SEQUENCE_ID=SSR_06338 Ps_contig_mira-and-tgicl-ass_franssen_44225
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACGGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTACTGTGTACTACTTCTAACC
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06339 Ps_contig_mira-and-tgicl-ass_franssen_44250
SEQUENCE=TTTAACTTATGAATTTTTAATTTCCATAACATAATTTAAATCCTAAGAGAAACACACTTCCAGATCAAAAACTTTAGACCAACATACCAACCTTGAAGGTTATATATCCACCATATTAATTAATTATAAAGTACATGTTAATAGTGTTTGTTTCCATGTGAGATGATCAAGAACTGCATTTGAAAATGTGTCTTAAACAACATGAAAGGATTAAAATGGTTTCATGGCACTGCTTCCATCTTCTCACATTCTTCTCGAACTTCTTGAAAAAGAGAACTGGACAAATACCTTTCGCCAAAGCTTGGAAATACAACCACAATAAGCTTTCCCTCATTTTCAGGTCTCTTTGCAACCTGTAATGCAGCGGTTGCAGCACAACCAGAAGAAATTCCCACCAACAAGCCTTCTTGAAGTGCTATTGTCCTTGCAGTTTTGACAGATTCTTCACCTGATATCGCTATAACTTCATCAAGGACTTCTTTATCTAAATTCTTGGGCACAAAACCAGCTCCAATACCCTGAATTATGTGAGGTCTTGGTGTTCCACCGCTAAGTATGTTACTTTCAAGAGGCTCTACACCAATGACCTTAATGTTTGGATTCTGTTCTTTTAGAAATCGACCTGCTCCAGAAAGGGTTCCACCAGTTCCAATTGCTGAAACTAAAATATCTACTTTACCTCTTGTATCTTCCCATATCTCTGGCCCAGTATTCTCATAATGTACTTTAGGATTGGATGGATTATCAAATTGTTGAAGCATGAATGCATTAGGTGTATTTTTTACAATTTCTTCGGCTTTTCGAACTGCTCCAGTCATAGCTTTGGAATATTCAGTCAAAACAAGTTCAGCTCCAAATGCTCTCATAAGAATTCTTCTTTCTAAACTCATCGAAACAGGCATTGTCAAGATGAGTTTATATCCTTTAGTAGCTGCTATGTAGGCAAGACCAATTCCTGTGTTACCACCGGTGGGTTCCACCAAAATACTCTTTCCGGGTGTTATGAGTCCTTTCCGCTCAGCATCAAGTATCATGCTATAACCGATCCTGTCTTTGACACTACAGCAGGGTTCCATAAGCTCAAGTCTTTGCAGACAATAGTAGCTACACAGCCTTTGGTGACACCCTTCAAGTAAACCATTTGGGTTGTTTT
TARGET=115,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.5146
PRIMER_LEFT_PENALTY=1.513860
PRIMER_RIGHT_PENALTY=0.000742
PRIMER_LEFT_SEQUENCE=AGACCAACATACCAACCTTGAA
PRIMER_RIGHT_SEQUENCE=GAGGGAAAGCTTATTGTGGTTG
PRIMER_LEFT=75,22
PRIMER_RIGHT=331,22
PRIMER_LEFT_TM=58.486
PRIMER_RIGHT_TM=60.001
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.3000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=257
PRIMER_PAIR_PENALTY_1=1.5146
PRIMER_LEFT_1_PENALTY=1.513860
PRIMER_RIGHT_1_PENALTY=0.000742
PRIMER_LEFT_1_SEQUENCE=AGACCAACATACCAACCTTGAA
PRIMER_RIGHT_1_SEQUENCE=TGAGGGAAAGCTTATTGTGGTT
PRIMER_LEFT_1=75,22
PRIMER_RIGHT_1=332,22
PRIMER_LEFT_1_TM=58.486
PRIMER_RIGHT_1_TM=60.001
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=258
PRIMER_PAIR_PENALTY_2=1.5211
PRIMER_LEFT_2_PENALTY=1.513860
PRIMER_RIGHT_2_PENALTY=0.007205
PRIMER_LEFT_2_SEQUENCE=AGACCAACATACCAACCTTGAA
PRIMER_RIGHT_2_SEQUENCE=GCGAAAGGTATTTGTCCAGTTC
PRIMER_LEFT_2=75,22
PRIMER_RIGHT_2=294,22
PRIMER_LEFT_2_TM=58.486
PRIMER_RIGHT_2_TM=60.007
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.3000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=220
PRIMER_PAIR_PENALTY_3=1.5727
PRIMER_LEFT_3_PENALTY=1.513860
PRIMER_RIGHT_3_PENALTY=0.058853
PRIMER_LEFT_3_SEQUENCE=AGACCAACATACCAACCTTGAA
PRIMER_RIGHT_3_SEQUENCE=CTGCATTACAGGTTGCAAAGAG
PRIMER_LEFT_3=75,22
PRIMER_RIGHT_3=363,22
PRIMER_LEFT_3_TM=58.486
PRIMER_RIGHT_3_TM=59.941
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=289
PRIMER_PAIR_PENALTY_4=1.6002
PRIMER_LEFT_4_PENALTY=1.513860
PRIMER_RIGHT_4_PENALTY=0.086339
PRIMER_LEFT_4_SEQUENCE=AGACCAACATACCAACCTTGAA
PRIMER_RIGHT_4_SEQUENCE=TACAGGTTGCAAAGAGACCTGA
PRIMER_LEFT_4=75,22
PRIMER_RIGHT_4=357,22
PRIMER_LEFT_4_TM=58.486
PRIMER_RIGHT_4_TM=59.914
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.3000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=283
=
PRIMER_SEQUENCE_ID=SSR_06340 Ps_contig_mira-and-tgicl-ass_franssen_44260
SEQUENCE=TGCCGCCTTCACGTTCTCTGGGCGGGCCGGCCCCCAACTGTTCTTCTAATTGTCGGTAATTTTAGGTAAGAGCTCTTGCGTATGAGAACGACACGTGCCATGGGTTTGGAGATTTGTTCATGGCATTCAAGTTGAGGGTTGCTTCAACCTCAGATTGTCCACCAGACAAAAACATGATACCAGGGACAGCAGGGGGAATTCTCCTGTGGAGGAGATTGAGGGTGTACTCGGCGACTTTTGTAGGAGAGGCTTTGTCCTTAGACTCAGCTCCTGGAGTAACCATGCTAGGCTTGAGGAGAATACCCTCAAATTGTACATTGTTCTCAGCAAGGTAGTAGAAAACTTCAGCCCAAACCTTTTGGGCTACTTCAAAAGTTCTGTCAATACCGTGTTCTCCATCAAGCAAGATCTCTGGCTCCACAATTGGGACCAATCCATTGTCCTGACTAATTGCAGCATAGCGAGCCAAACCCCAAGCCGCTTCCTTGACAGCAAGAGCAGAAGGACCATTGGGGATGCTCACGACAGTACGCCATTTGGCGAAACGGGCACCTTGCTGGTAGTATGCAGCCGAACGAGAGGCAAGACCGTCTAGACCTTGGCACCATGATTCATTGTTGGAACCAGCTAGGGGCACTAGACCCTTGTCAACTTTAATACCGGGAATAATGTTTTGCTCGACAAGCACATCGACGATCTTCCTTCCGTCAACTGTGGATTGGTAGAGAGTCTCCTCAAAGAGAATGGCACCAGAGATGTACTCTCCAAGTGTTGGAACTGTCACCAGAAGTGTACGCCATGCTTGACGGTTAGCCTCGGTGTTCTCTAGCCCAATTGAATCCAAACGCTTTCCACATGTAGCATTGGACTCATCCATGGCCAAAATACCACGCCCTGGTGAAGCAATTGTTTTCGCAGTTTTGACGAGCTCGTCGGCATAGGAACCAGCTCGGATGGTAAAACTTGATGAATTAGCAGGGTTGCATCTGGCAACAGACATAGAAGAAGGTTGACGGAGGAGAGTTTTTCCCTTCACCCATTCAGACTTATCAAGAACAAGTGATGATGATTTGAAAATAGATGCTGAAGCAGAAGCCATAGTTTCACAAACTCACTCACTCACTGCAATCTTATCCCCACAACAACAACCTCTTTACCACCCCCGANTACTCTGCGTTGTATTACCTANTNCTT
TARGET=1110,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1509
PRIMER_LEFT_PENALTY=0.006951
PRIMER_RIGHT_PENALTY=0.143968
PRIMER_LEFT_SEQUENCE=ATGAATTAGCAGGGTTGCATCT
PRIMER_RIGHT_SEQUENCE=AGGTTGTTGTTGTGGGGATAAG
PRIMER_LEFT=966,22
PRIMER_RIGHT=1150,22
PRIMER_LEFT_TM=59.993
PRIMER_RIGHT_TM=60.144
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=5.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=185
PRIMER_PAIR_PENALTY_1=0.2621
PRIMER_LEFT_1_PENALTY=0.118120
PRIMER_RIGHT_1_PENALTY=0.143968
PRIMER_LEFT_1_SEQUENCE=CCACATGTAGCATTGGACTCAT
PRIMER_RIGHT_1_SEQUENCE=AGGTTGTTGTTGTGGGGATAAG
PRIMER_LEFT_1=851,22
PRIMER_RIGHT_1=1150,22
PRIMER_LEFT_1_TM=59.882
PRIMER_RIGHT_1_TM=60.144
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=5.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=300
PRIMER_PAIR_PENALTY_2=0.2647
PRIMER_LEFT_2_PENALTY=0.120688
PRIMER_RIGHT_2_PENALTY=0.143968
PRIMER_LEFT_2_SEQUENCE=TAGAAGAAGGTTGACGGAGGAG
PRIMER_RIGHT_2_SEQUENCE=AGGTTGTTGTTGTGGGGATAAG
PRIMER_LEFT_2=999,22
PRIMER_RIGHT_2=1150,22
PRIMER_LEFT_2_TM=59.879
PRIMER_RIGHT_2_TM=60.144
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=152
PRIMER_PAIR_PENALTY_3=0.2672
PRIMER_LEFT_3_PENALTY=0.123278
PRIMER_RIGHT_3_PENALTY=0.143968
PRIMER_LEFT_3_SEQUENCE=CATAGAAGAAGGTTGACGGAGG
PRIMER_RIGHT_3_SEQUENCE=AGGTTGTTGTTGTGGGGATAAG
PRIMER_LEFT_3=997,22
PRIMER_RIGHT_3=1150,22
PRIMER_LEFT_3_TM=60.123
PRIMER_RIGHT_3_TM=60.144
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.4000
PRIMER_RIGHT_3_END_STABILITY=5.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=154
PRIMER_PAIR_PENALTY_4=0.2758
PRIMER_LEFT_4_PENALTY=0.000037
PRIMER_RIGHT_4_PENALTY=0.275749
PRIMER_LEFT_4_SEQUENCE=TTCCACATGTAGCATTGGACTC
PRIMER_RIGHT_4_SEQUENCE=TGTTGTTGTGGGGATAAGATTG
PRIMER_LEFT_4=849,22
PRIMER_RIGHT_4=1146,22
PRIMER_LEFT_4_TM=60.000
PRIMER_RIGHT_4_TM=59.724
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.1000
PRIMER_RIGHT_4_END_STABILITY=6.9000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=298
=
PRIMER_SEQUENCE_ID=SSR_06341 Ps_contig_mira-and-tgicl-ass_franssen_44347
SEQUENCE=TACATGGGGAGTTTTGTTTGAAAGGCTACAGAGTTTTGGTTAGGTTAGGTTACCCCTTCGGTCGAAGGACTAGACCCACTTTACCCTGGTGGTGCTTTTGACCCTCTTGGCCTTGCTGATGACCCTGATTCATTTGCAGAGTTGAAGGTCAAGGAACTCAAGAATGGTCGGCTGGCTATGTTCTCCATGTTTGGTTTCTTTGTTCAAGCCATTGTTACTGGCAAGGGCCCTATTCAGAACCTTTATGACCATGTTGCTGACCCTGTCGCGAACAACGCATGGG
TARGET=38,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3584
PRIMER_LEFT_PENALTY=0.354011
PRIMER_RIGHT_PENALTY=0.004359
PRIMER_LEFT_SEQUENCE=GGGAGTTTTGTTTGAAAGGCTA
PRIMER_RIGHT_SEQUENCE=AACTCTGCAAATGAATCAGGGT
PRIMER_LEFT=6,22
PRIMER_RIGHT=142,22
PRIMER_LEFT_TM=59.646
PRIMER_RIGHT_TM=60.004
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=8.7000
PRIMER_RIGHT_END_STABILITY=9.1000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=137
PRIMER_PAIR_PENALTY_1=0.3752
PRIMER_LEFT_1_PENALTY=0.354011
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=GGGAGTTTTGTTTGAAAGGCTA
PRIMER_RIGHT_1_SEQUENCE=GGCTTGAACAAAGAAACCAAAC
PRIMER_LEFT_1=6,22
PRIMER_RIGHT_1=209,22
PRIMER_LEFT_1_TM=59.646
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=204
PRIMER_PAIR_PENALTY_2=0.6029
PRIMER_LEFT_2_PENALTY=0.354011
PRIMER_RIGHT_2_PENALTY=0.248900
PRIMER_LEFT_2_SEQUENCE=GGGAGTTTTGTTTGAAAGGCTA
PRIMER_RIGHT_2_SEQUENCE=TCAACTCTGCAAATGAATCAGG
PRIMER_LEFT_2=6,22
PRIMER_RIGHT_2=144,22
PRIMER_LEFT_2_TM=59.646
PRIMER_RIGHT_2_TM=60.249
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.7000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=0.6377
PRIMER_LEFT_3_PENALTY=0.354011
PRIMER_RIGHT_3_PENALTY=0.283676
PRIMER_LEFT_3_SEQUENCE=GGGAGTTTTGTTTGAAAGGCTA
PRIMER_RIGHT_3_SEQUENCE=TTGACCTTCAACTCTGCAAATG
PRIMER_LEFT_3=6,22
PRIMER_RIGHT_3=151,22
PRIMER_LEFT_3_TM=59.646
PRIMER_RIGHT_3_TM=60.284
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.7000
PRIMER_RIGHT_3_END_STABILITY=7.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=146
PRIMER_PAIR_PENALTY_4=0.6429
PRIMER_LEFT_4_PENALTY=0.354011
PRIMER_RIGHT_4_PENALTY=0.288917
PRIMER_LEFT_4_SEQUENCE=GGGAGTTTTGTTTGAAAGGCTA
PRIMER_RIGHT_4_SEQUENCE=GTTCCTTGACCTTCAACTCTGC
PRIMER_LEFT_4=6,22
PRIMER_RIGHT_4=156,22
PRIMER_LEFT_4_TM=59.646
PRIMER_RIGHT_4_TM=60.289
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=151
=
PRIMER_SEQUENCE_ID=SSR_06342 Ps_contig_mira-and-tgicl-ass_franssen_44379
SEQUENCE=CTAACCTCCAACTCCTTCTTCTTTCCTTATTCAAAAACCCCCCAAAAACCCCTAAANCGCGTCCATGTTCAAACAAAACGACTTCAATTTCAGCATCAATCCTTCACCATTATCAACCTCACCGTCGCCATCACCCTCAGCAACATCCTCTTTCACACGGGTGAGTGCAGCAAGAGCAGCTTCAGTACGTTTATCTATATCACGAAGCGCGATCTGAAGCGCGTCGTCGAGAGTTGATTCACGGTGACGGAGAACATTAAGCGACTCGTTAGTTTGGTTATCGAGAGAACAAATTTTCCGATTAAGCGCTTCAGATTTGTTGAGAAGGTCTTGCTCGAGAGATGAGAAGTGGTCGGAGAGTTCCTTCCAGAGGAGGGTGCAGCTAGTCATGAGGGAGGTTTGGCGTTGGAAGTCATCGAAGCTGGGTTGAGCCAACTCGGTTAACTCGCCGGGATCGGGGATAGACCCCATGGTGGAAGAAGAAGAAGAAGAAAACCCTAATTTAGTTCAAATCAGAGAAAGAGATAGAAGAGAAAGAGAAGAACGAAGAACGAAGAAAGAAGGAAAGAAAAGAGTGTCTGCGTTAG
TARGET=476,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1588
PRIMER_LEFT_PENALTY=0.153161
PRIMER_RIGHT_PENALTY=0.005674
PRIMER_LEFT_SEQUENCE=AAGGTCTTGCTCGAGAGATGAG
PRIMER_RIGHT_SEQUENCE=TTCCTTCTTTCTTCGTTCTTCG
PRIMER_LEFT=324,22
PRIMER_RIGHT=565,22
PRIMER_LEFT_TM=60.153
PRIMER_RIGHT_TM=59.994
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.6000
PRIMER_RIGHT_END_STABILITY=8.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.1588
PRIMER_LEFT_1_PENALTY=0.153161
PRIMER_RIGHT_1_PENALTY=0.005674
PRIMER_LEFT_1_SEQUENCE=AGAAGGTCTTGCTCGAGAGATG
PRIMER_RIGHT_1_SEQUENCE=TTCCTTCTTTCTTCGTTCTTCG
PRIMER_LEFT_1=322,22
PRIMER_RIGHT_1=565,22
PRIMER_LEFT_1_TM=60.153
PRIMER_RIGHT_1_TM=59.994
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=2.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.6000
PRIMER_RIGHT_1_END_STABILITY=8.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=244
PRIMER_PAIR_PENALTY_2=0.2072
PRIMER_LEFT_2_PENALTY=0.153161
PRIMER_RIGHT_2_PENALTY=0.054021
PRIMER_LEFT_2_SEQUENCE=AGAAGGTCTTGCTCGAGAGATG
PRIMER_RIGHT_2_SEQUENCE=CGCAGACACTCTTTTCTTTCCT
PRIMER_LEFT_2=322,22
PRIMER_RIGHT_2=582,22
PRIMER_LEFT_2_TM=60.153
PRIMER_RIGHT_2_TM=60.054
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.6000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=261
PRIMER_PAIR_PENALTY_3=0.2072
PRIMER_LEFT_3_PENALTY=0.153161
PRIMER_RIGHT_3_PENALTY=0.054021
PRIMER_LEFT_3_SEQUENCE=AAGGTCTTGCTCGAGAGATGAG
PRIMER_RIGHT_3_SEQUENCE=CGCAGACACTCTTTTCTTTCCT
PRIMER_LEFT_3=324,22
PRIMER_RIGHT_3=582,22
PRIMER_LEFT_3_TM=60.153
PRIMER_RIGHT_3_TM=60.054
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=259
PRIMER_PAIR_PENALTY_4=0.2248
PRIMER_LEFT_4_PENALTY=0.153161
PRIMER_RIGHT_4_PENALTY=0.071684
PRIMER_LEFT_4_SEQUENCE=AGAAGGTCTTGCTCGAGAGATG
PRIMER_RIGHT_4_SEQUENCE=ACGCAGACACTCTTTTCTTTCC
PRIMER_LEFT_4=322,22
PRIMER_RIGHT_4=583,22
PRIMER_LEFT_4_TM=60.153
PRIMER_RIGHT_4_TM=59.928
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.6000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=262
=
PRIMER_SEQUENCE_ID=SSR_06343 Ps_contig_mira-and-tgicl-ass_franssen_44438
SEQUENCE=TTAACTTTGAATGTGCATCATTTATCATGGTATCATCAGTTACCATTGTCGGAAGCACCACCATGTCCACCATGATGACCACCACCATGACCGTAGTGACCACCACCATAGTGTCCACCTCCATAGTGTCCACCACCATAGTGTCCACCGCCATATTTGGCATCATTTACTTCATTTGACTTTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCGCCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGTCCATGACCATAGCCTCCATGTCCATGACCATAGCCATGACCATAACCTCCACCGTAATATTTGGCATCATTTACTTCATTTGACTTTTCAGCAACTTCCTCTTTGGCATTAGTGGAAGTCTCAGCTAATTCTCTAGCTAGAAACCTCGGAAGGAAATAACAAGAA
TARGET=246,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6759
PRIMER_LEFT_PENALTY=0.295944
PRIMER_RIGHT_PENALTY=0.379939
PRIMER_LEFT_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_RIGHT_SEQUENCE=AGGTTATGGTCATGGCTATGGT
PRIMER_LEFT=109,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=60.296
PRIMER_RIGHT_TM=59.620
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.7086
PRIMER_LEFT_1_PENALTY=0.621889
PRIMER_RIGHT_1_PENALTY=0.086698
PRIMER_LEFT_1_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_1_SEQUENCE=AGCTGAGACTTCCACTAATGCC
PRIMER_LEFT_1=184,22
PRIMER_RIGHT_1=449,22
PRIMER_LEFT_1_TM=59.378
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.7787
PRIMER_LEFT_2_PENALTY=0.295944
PRIMER_RIGHT_2_PENALTY=0.482784
PRIMER_LEFT_2_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_RIGHT_2_SEQUENCE=ATTACGGTGGAGGTTATGGTCA
PRIMER_LEFT_2=109,22
PRIMER_RIGHT_2=381,22
PRIMER_LEFT_2_TM=60.296
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.8619
PRIMER_LEFT_3_PENALTY=0.295944
PRIMER_RIGHT_3_PENALTY=0.565974
PRIMER_LEFT_3_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_RIGHT_3_SEQUENCE=GATGCCAAATATTACGGTGGAG
PRIMER_LEFT_3=109,22
PRIMER_RIGHT_3=391,22
PRIMER_LEFT_3_TM=60.296
PRIMER_RIGHT_3_TM=60.566
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.8754
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=0.253482
PRIMER_LEFT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=TGAGACTTCCACTAATGCCAAA
PRIMER_LEFT_4=184,22
PRIMER_RIGHT_4=446,22
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=59.747
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_06344 Ps_contig_mira-and-tgicl-ass_franssen_44438
SEQUENCE=TTAACTTTGAATGTGCATCATTTATCATGGTATCATCAGTTACCATTGTCGGAAGCACCACCATGTCCACCATGATGACCACCACCATGACCGTAGTGACCACCACCATAGTGTCCACCTCCATAGTGTCCACCACCATAGTGTCCACCGCCATATTTGGCATCATTTACTTCATTTGACTTTTCAACAACCTCATTGTTGGAAACACCGCCACCGTTGTATCCACCACCGCCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCACCACCACCGCCATGACCGTAACCTCCACCACCATGACCGTAGCCTCCATGTCCATGACCATAGCCTCCATGTCCATGACCATAGCCATGACCATAACCTCCACCGTAATATTTGGCATCATTTACTTCATTTGACTTTTCAGCAACTTCCTCTTTGGCATTAGTGGAAGTCTCAGCTAATTCTCTAGCTAGAAACCTCGGAAGGAAATAACAAGAA
TARGET=270,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6759
PRIMER_LEFT_PENALTY=0.295944
PRIMER_RIGHT_PENALTY=0.379939
PRIMER_LEFT_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_RIGHT_SEQUENCE=AGGTTATGGTCATGGCTATGGT
PRIMER_LEFT=109,22
PRIMER_RIGHT=371,22
PRIMER_LEFT_TM=60.296
PRIMER_RIGHT_TM=59.620
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=7.8000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=263
PRIMER_PAIR_PENALTY_1=0.7086
PRIMER_LEFT_1_PENALTY=0.621889
PRIMER_RIGHT_1_PENALTY=0.086698
PRIMER_LEFT_1_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_1_SEQUENCE=AGCTGAGACTTCCACTAATGCC
PRIMER_LEFT_1=184,22
PRIMER_RIGHT_1=449,22
PRIMER_LEFT_1_TM=59.378
PRIMER_RIGHT_1_TM=59.913
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.6000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=266
PRIMER_PAIR_PENALTY_2=0.7787
PRIMER_LEFT_2_PENALTY=0.295944
PRIMER_RIGHT_2_PENALTY=0.482784
PRIMER_LEFT_2_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_RIGHT_2_SEQUENCE=ATTACGGTGGAGGTTATGGTCA
PRIMER_LEFT_2=109,22
PRIMER_RIGHT_2=381,22
PRIMER_LEFT_2_TM=60.296
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=273
PRIMER_PAIR_PENALTY_3=0.8619
PRIMER_LEFT_3_PENALTY=0.295944
PRIMER_RIGHT_3_PENALTY=0.565974
PRIMER_LEFT_3_SEQUENCE=AGTGTCCACCTCCATAGTGTCC
PRIMER_RIGHT_3_SEQUENCE=GATGCCAAATATTACGGTGGAG
PRIMER_LEFT_3=109,22
PRIMER_RIGHT_3=391,22
PRIMER_LEFT_3_TM=60.296
PRIMER_RIGHT_3_TM=60.566
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.8754
PRIMER_LEFT_4_PENALTY=0.621889
PRIMER_RIGHT_4_PENALTY=0.253482
PRIMER_LEFT_4_SEQUENCE=CAACAACCTCATTGTTGGAAAC
PRIMER_RIGHT_4_SEQUENCE=TGAGACTTCCACTAATGCCAAA
PRIMER_LEFT_4=184,22
PRIMER_RIGHT_4=446,22
PRIMER_LEFT_4_TM=59.378
PRIMER_RIGHT_4_TM=59.747
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=7.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.8000
PRIMER_PAIR_4_COMPL_ANY=7.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_06345 Ps_contig_mira-and-tgicl-ass_franssen_44475
SEQUENCE=TCGGGGAAGAGTAAAACGTAGAAACTTTTAGTACCAGAGTTTTGGGAGAGTGATATGGCACAGTGGTCGTTGCTTATCGTTTTGTTCTGCGTCGCCTCCGCCGCCGCCGGATTCAGCTTCCATGACTCCAATCCTATTCGAATGGTCTCCGACGTGGAGGAGCAGTTACTTCAGGTGATCGGAGAAGTCGTCACGCCGTCTCTTTCGCCCGGTTCGCTAACAGATACGGTAAGCGATACGATAG
TARGET=98,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.1540
PRIMER_LEFT_PENALTY=0.122257
PRIMER_RIGHT_PENALTY=0.031769
PRIMER_LEFT_SEQUENCE=TCGGGGAAGAGTAAAACGTAGA
PRIMER_RIGHT_SEQUENCE=ATTGGAGTCATGGAAGCTGAAT
PRIMER_LEFT=0,22
PRIMER_RIGHT=131,22
PRIMER_LEFT_TM=60.122
PRIMER_RIGHT_TM=59.968
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=5.4000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=2.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=132
PRIMER_PAIR_PENALTY_1=0.3235
PRIMER_LEFT_1_PENALTY=0.122257
PRIMER_RIGHT_1_PENALTY=0.201288
PRIMER_LEFT_1_SEQUENCE=TCGGGGAAGAGTAAAACGTAGA
PRIMER_RIGHT_1_SEQUENCE=GACCATTCGAATAGGATTGGAG
PRIMER_LEFT_1=0,22
PRIMER_RIGHT_1=146,22
PRIMER_LEFT_1_TM=60.122
PRIMER_RIGHT_1_TM=59.799
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=5.4000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=0.3235
PRIMER_LEFT_2_PENALTY=0.122257
PRIMER_RIGHT_2_PENALTY=0.201288
PRIMER_LEFT_2_SEQUENCE=TCGGGGAAGAGTAAAACGTAGA
PRIMER_RIGHT_2_SEQUENCE=CCATTCGAATAGGATTGGAGTC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=144,22
PRIMER_LEFT_2_TM=60.122
PRIMER_RIGHT_2_TM=59.799
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.1000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=145
PRIMER_PAIR_PENALTY_3=0.3637
PRIMER_LEFT_3_PENALTY=0.061818
PRIMER_RIGHT_3_PENALTY=0.301926
PRIMER_LEFT_3_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_3_SEQUENCE=CCTGAAGTAACTGCTCCTCCAC
PRIMER_LEFT_3=61,22
PRIMER_RIGHT_3=174,22
PRIMER_LEFT_3_TM=60.062
PRIMER_RIGHT_3_TM=60.302
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=0.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=114
PRIMER_PAIR_PENALTY_4=0.3637
PRIMER_LEFT_4_PENALTY=0.061818
PRIMER_RIGHT_4_PENALTY=0.301926
PRIMER_LEFT_4_SEQUENCE=AGTGGTCGTTGCTTATCGTTTT
PRIMER_RIGHT_4_SEQUENCE=CACCTGAAGTAACTGCTCCTCC
PRIMER_LEFT_4=61,22
PRIMER_RIGHT_4=176,22
PRIMER_LEFT_4_TM=60.062
PRIMER_RIGHT_4_TM=60.302
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=54.545
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=7.0000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=116
=
PRIMER_SEQUENCE_ID=SSR_06346 Ps_contig_mira-and-tgicl-ass_franssen_44482
SEQUENCE=AAAAGAAACAAAGTAAGGTAATACAACGCAGATANTCGGGGACATTCAAACCAAATTCATCAACCAACAACCAAACATGTTTCAAATCATTTTCCTCCTCTCCCTTTTCCTCTCCATTTCCAATGCTTCTGTCCAAGACTTCTGTGTAGCAGATCTCAAAGCCTCAGACACCCCATCAGGCTTCCCTTGCAAGTCAGCAACAACAGTAACCGTAGAAGACTTTGCATTTTCAGGCTTAATTGAGCCAGCAAACGTAACAAACATAATCAACGCCGCCGTTACGCCTGCATTTGTCGCACAATTTCCAGGCCTTAATGGCCTTGGTCTCTCAGCAGCTAGGTTAGACCTTGGTCCTGCAGGTGTTATCCCTCTACACAGTCACCCGGGTGCAAGTGAGCTTCTTATTGTGACACAAGGTCACATCACTGCTGGATTTGTTTCTTCAGCTAACACTGTTTATATTAAAACTTTGAAGAAAGGTGAACTCATGGTTTTTCCACAAGGTTTGTTGCATTTTCAAGCTACTGCTGGTAAGAGAAAAGCTGTTGCTTTTCCTGTTTTTAGTAGTGCTAGTCCTGGTCTTCAGATTCTTGATTTTGCACTCTTTGCAAGCAATTTCTCTACTCCTTTGATTGCAAAGACCACTTTCCTTGATCCTGTTCTTATCAAGAAACTCAAGAGTGTTCTTGGTGGAAGCGGCTAATTAAGCCTTATTTATATCTATATATGTAATTTATTTTTCTAAGTAATGCTTATGCTTATAATAGTTGTTATTTATCAACCTATTTTCTTGTATTATTTCTTCTTCTTCTTCTTCATCNTCCTCTNTATNATCT
TARGET=800,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06347 Ps_contig_mira-and-tgicl-ass_franssen_44485
SEQUENCE=GACTCGCAACGCAGAGTCGGGGACTCCACTCAACAATGTCTCCAAACAAAGCCTTCATCTTCTTAGCTCTCTTATCCTTTTCACCACAACTTTTCCTAACAATCTCTTCTGCTGTAGAAGATAACGGCCTTCTCTTAAACTACTACAAAGAATCATGTCCACAAGCTGAAGAAATCATCAAAGAACAAGTCAAACTTCTCTACAAACGCCACAAGAACACCGCTTTCTCATGGCTCAGAAACATTTTCCATGACTGTGCTGTTCAGAGCTGTGATGCTTCTTTGTTGCTGACATCCACTAGAAGAAGCTTGTCTGAACAAGAACATGACAGAAGCTTTGGTTTGAGGAATTTTAGGTACATTGATACCATCAAAGAAGCTGTTGAAAGAGAGTGTCCCGGTGTTGTTTCTTGTTCTGATATCCTTGTTCTTTCAGCTAGAGATGGAATTGTTTCGCTAGGAGGTCCTTATATTCCATTGAAAACCGGAAGAAGAGATGGAAGAAAGAGCAGAGTGGATCTGTTGGAGGCGTACCTTCCTGATCACAATGAATCTATTTCTTCTGTTCTTGACAAGTTCGGTGCCATGGGAATCGACACTCCTGGAGTTGTTTCTTTGCTGGGAGCACACAGTGTTGGTAGAACTCATTGCACAAAACTGGTGCACCGTTTATATCCAGAAGTTGATCCAGCTTTGAATCCAGATCACATTCCACACATGCTAAAGAAGTGTCCTGATTCAATCCCTGACCCTAAGGCAGTACAGTACGTGAGAAATGACCGTGGTACCCCCATGATTCTTGATAACAATTACTATAGAAATATTCTCGACAACAAGGGTCTTTTAATAGTGGATCATCAACTAGCACATGACAAAAGGACAAAACCTTATGTGAAGAAAATGGCCAAAAGTCAAGACTATTTCTTCAAGGAGTTTTCTAAAGCTATTACATTGCTTTCTGAGAATAATCCTCTAACCGGTACTAAAGGTGAGATTAGAAAACAGTGCAGTGTTGCTAACAAACAACACCATGATGAGCCTTGAATGAAAAATTCATGGTGGATGAAGAAGAAGTCATGTGTAAGATATTATTATGCAGAAATAAAAGTGTTCATGGAGATTCAAGAACTTAGATGCTTTTAATTTTTAATTGGAACTTTGTTTTTTATTTATTTAATATTAGTAGTATGGTAGCTCAATGTGGAGCATGTGGATGATGTGATGTATGGTATTTCATGTGGTGCATGATGGCATGTAGTGACAATGTTTATGATATTTGTGCTCATAAAGATTGTGTTCTTTTATATGACAAAAAACT
TARGET=1164,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.5160
PRIMER_LEFT_PENALTY=0.095523
PRIMER_RIGHT_PENALTY=0.420521
PRIMER_LEFT_SEQUENCE=AACAAACAACACCATGATGAGC
PRIMER_RIGHT_SEQUENCE=ATCATGCACCACATGAAATACC
PRIMER_LEFT=1016,22
PRIMER_RIGHT=1247,22
PRIMER_LEFT_TM=59.904
PRIMER_RIGHT_TM=59.579
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=6.8000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=232
PRIMER_PAIR_PENALTY_1=0.7533
PRIMER_LEFT_1_PENALTY=0.095523
PRIMER_RIGHT_1_PENALTY=0.657742
PRIMER_LEFT_1_SEQUENCE=AACAAACAACACCATGATGAGC
PRIMER_RIGHT_1_SEQUENCE=TGCTCCACATTGAGCTACCATA
PRIMER_LEFT_1=1016,22
PRIMER_RIGHT_1=1206,22
PRIMER_LEFT_1_TM=59.904
PRIMER_RIGHT_1_TM=60.658
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.4000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=191
PRIMER_PAIR_PENALTY_2=0.8152
PRIMER_LEFT_2_PENALTY=0.095523
PRIMER_RIGHT_2_PENALTY=0.719637
PRIMER_LEFT_2_SEQUENCE=AACAAACAACACCATGATGAGC
PRIMER_RIGHT_2_SEQUENCE=CACATGCTCCACATTGAGCTAC
PRIMER_LEFT_2=1016,22
PRIMER_RIGHT_2=1210,22
PRIMER_LEFT_2_TM=59.904
PRIMER_RIGHT_2_TM=60.720
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=195
PRIMER_PAIR_PENALTY_3=1.1731
PRIMER_LEFT_3_PENALTY=0.752572
PRIMER_RIGHT_3_PENALTY=0.420521
PRIMER_LEFT_3_SEQUENCE=AACACCATGATGAGCCTTGAAT
PRIMER_RIGHT_3_SEQUENCE=ATCATGCACCACATGAAATACC
PRIMER_LEFT_3=1023,22
PRIMER_RIGHT_3=1247,22
PRIMER_LEFT_3_TM=60.753
PRIMER_RIGHT_3_TM=59.579
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.8000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=225
PRIMER_PAIR_PENALTY_4=1.2028
PRIMER_LEFT_4_PENALTY=0.782312
PRIMER_RIGHT_4_PENALTY=0.420521
PRIMER_LEFT_4_SEQUENCE=AAACAACACCATGATGAGCCTT
PRIMER_RIGHT_4_SEQUENCE=ATCATGCACCACATGAAATACC
PRIMER_LEFT_4=1019,22
PRIMER_RIGHT_4=1247,22
PRIMER_LEFT_4_TM=60.782
PRIMER_RIGHT_4_TM=59.579
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=6.8000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=229
=
PRIMER_SEQUENCE_ID=SSR_06348 Ps_contig_mira-and-tgicl-ass_franssen_44486
SEQUENCE=AAAGACAAGTAGGTAATCARYTCGGRGTCGGGGTACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCAACCCCCAAAACATGGAAACAACCCTCCACTCTATCTTTCATCTCTCTCAAACCAACCAAGTTCACACCAAGAACAACAAAAATTGCTGCAACAGAAGACAAAACCGATGCAGCAACCGATGTAGCAACAAAAGAAGCACCAGTTGGATTCCACCCCAC
TARGET=39,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06349 Ps_contig_mira-and-tgicl-ass_franssen_44512
SEQUENCE=GGTTGGGAACGAAATAGGAACAGTTGTATGCATTTAACTAACAAAGTAGTGGCGTATCAAGATTATGGTGAGGCTGACATCAAAACTGGTGCACAAATTACCAATGGAGGTTACACTAATCTTAGTAAACAATGGAACCAAACATGCATTCAACAACACTGCAGGAACTCAGAATTTTGGAAAGGCCAAGATCAACACTGGAGCCAGGATTGGAAATTAAATCATTACTATCAGCTTTGAGTGGGGTCCTTTTACCCAAAACATATACATATAATATAATATAATAAGTGTATGTNTATTGTTTTGGTAATATAGTAATAAATAAGTTNTTTATCTNTNTCTTATGAGTGTTTG
TARGET=269,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06350 Ps_contig_mira-and-tgicl-ass_franssen_44560
SEQUENCE=CYCGGGTCGGGCAACAACAAAACCTATTTTGAGAAAACATGGGGTCTCAAATGACCTCTATTACTCTCACTATTGCATTGACAATACTCTCTCTCACCTTGCCATCTCAAGCAAACAATTACCTCTATTCATCTCCACCACCACCACCAAAACCTTACTACTACCAATCTCCACCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCACCGGTTTACAAATCTCCCCCTCCACCATACAAATATCAATCTCCACCACCGCCTCCTTACAAGTACTCATCACCACCTCCACCAGTCTACAAGTACAATTCACCCCCTCCTCCTTACAAGCACATATCTCCTCCAACACCTGGAAAGCCATTCAAGTTCCCACCTCCACCAACACCGATTTACAAATATAAATCTCCTCCACCTGTTTACTCGCCACCACCCGTTTACAAATATAAATCTCCTCCTCCACCTGTTTACTCACCACCTCCACCACACTATGTCTACTCTTCACCTCCTCCACCGGTCTATTCCCCTCCTCCACCTCATTACATATATGCCTCACCTCCTCCTCCTTACCACTACTAGATAGTGGCTTACCGTAGTATCCCATGGATTTGAAGAATATATATAATAATATTGGAAATAAAGAGAACATAGTGCAAAGCAGGAAGATGCATTTCAAAATATACAAGGAAAAAACAACGGAAGGAAAGTTTCCATGCACTTGGTCTCAAGAAAAGTCATTATTCTTTTCCCATTTTGTTTTGTTATGAATTGTTATTCTCATTATTGGAGTGCTTGGTTTTTGAATTTAGACAATTGTAAGGCACTGTGTGTGTAATATCATAAACGAATAAAGCTTTTTATTTAGCAAACTAACTANNAAAAAA
TARGET=135,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06351 Ps_contig_mira-and-tgicl-ass_franssen_44560
SEQUENCE=CYCGGGTCGGGCAACAACAAAACCTATTTTGAGAAAACATGGGGTCTCAAATGACCTCTATTACTCTCACTATTGCATTGACAATACTCTCTCTCACCTTGCCATCTCAAGCAAACAATTACCTCTATTCATCTCCACCACCACCACCAAAACCTTACTACTACCAATCTCCACCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCACCGGTTTACAAATCTCCCCCTCCACCATACAAATATCAATCTCCACCACCGCCTCCTTACAAGTACTCATCACCACCTCCACCAGTCTACAAGTACAATTCACCCCCTCCTCCTTACAAGCACATATCTCCTCCAACACCTGGAAAGCCATTCAAGTTCCCACCTCCACCAACACCGATTTACAAATATAAATCTCCTCCACCTGTTTACTCGCCACCACCCGTTTACAAATATAAATCTCCTCCTCCACCTGTTTACTCACCACCTCCACCACACTATGTCTACTCTTCACCTCCTCCACCGGTCTATTCCCCTCCTCCACCTCATTACATATATGCCTCACCTCCTCCTCCTTACCACTACTAGATAGTGGCTTACCGTAGTATCCCATGGATTTGAAGAATATATATAATAATATTGGAAATAAAGAGAACATAGTGCAAAGCAGGAAGATGCATTTCAAAATATACAAGGAAAAAACAACGGAAGGAAAGTTTCCATGCACTTGGTCTCAAGAAAAGTCATTATTCTTTTCCCATTTTGTTTTGTTATGAATTGTTATTCTCATTATTGGAGTGCTTGGTTTTTGAATTTAGACAATTGTAAGGCACTGTGTGTGTAATATCATAAACGAATAAAGCTTTTTATTTAGCAAACTAACTANNAAAAAA
TARGET=217,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06352 Ps_contig_mira-and-tgicl-ass_franssen_44560
SEQUENCE=CYCGGGTCGGGCAACAACAAAACCTATTTTGAGAAAACATGGGGTCTCAAATGACCTCTATTACTCTCACTATTGCATTGACAATACTCTCTCTCACCTTGCCATCTCAAGCAAACAATTACCTCTATTCATCTCCACCACCACCACCAAAACCTTACTACTACCAATCTCCACCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCACCGGTTTACAAATCTCCCCCTCCACCATACAAATATCAATCTCCACCACCGCCTCCTTACAAGTACTCATCACCACCTCCACCAGTCTACAAGTACAATTCACCCCCTCCTCCTTACAAGCACATATCTCCTCCAACACCTGGAAAGCCATTCAAGTTCCCACCTCCACCAACACCGATTTACAAATATAAATCTCCTCCACCTGTTTACTCGCCACCACCCGTTTACAAATATAAATCTCCTCCTCCACCTGTTTACTCACCACCTCCACCACACTATGTCTACTCTTCACCTCCTCCACCGGTCTATTCCCCTCCTCCACCTCATTACATATATGCCTCACCTCCTCCTCCTTACCACTACTAGATAGTGGCTTACCGTAGTATCCCATGGATTTGAAGAATATATATAATAATATTGGAAATAAAGAGAACATAGTGCAAAGCAGGAAGATGCATTTCAAAATATACAAGGAAAAAACAACGGAAGGAAAGTTTCCATGCACTTGGTCTCAAGAAAAGTCATTATTCTTTTCCCATTTTGTTTTGTTATGAATTGTTATTCTCATTATTGGAGTGCTTGGTTTTTGAATTTAGACAATTGTAAGGCACTGTGTGTGTAATATCATAAACGAATAAAGCTTTTTATTTAGCAAACTAACTANNAAAAAA
TARGET=265,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06353 Ps_contig_mira-and-tgicl-ass_franssen_44560
SEQUENCE=CYCGGGTCGGGCAACAACAAAACCTATTTTGAGAAAACATGGGGTCTCAAATGACCTCTATTACTCTCACTATTGCATTGACAATACTCTCTCTCACCTTGCCATCTCAAGCAAACAATTACCTCTATTCATCTCCACCACCACCACCAAAACCTTACTACTACCAATCTCCACCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCACCGGTTTACAAATCTCCCCCTCCACCATACAAATATCAATCTCCACCACCGCCTCCTTACAAGTACTCATCACCACCTCCACCAGTCTACAAGTACAATTCACCCCCTCCTCCTTACAAGCACATATCTCCTCCAACACCTGGAAAGCCATTCAAGTTCCCACCTCCACCAACACCGATTTACAAATATAAATCTCCTCCACCTGTTTACTCGCCACCACCCGTTTACAAATATAAATCTCCTCCTCCACCTGTTTACTCACCACCTCCACCACACTATGTCTACTCTTCACCTCCTCCACCGGTCTATTCCCCTCCTCCACCTCATTACATATATGCCTCACCTCCTCCTCCTTACCACTACTAGATAGTGGCTTACCGTAGTATCCCATGGATTTGAAGAATATATATAATAATATTGGAAATAAAGAGAACATAGTGCAAAGCAGGAAGATGCATTTCAAAATATACAAGGAAAAAACAACGGAAGGAAAGTTTCCATGCACTTGGTCTCAAGAAAAGTCATTATTCTTTTCCCATTTTGTTTTGTTATGAATTGTTATTCTCATTATTGGAGTGCTTGGTTTTTGAATTTAGACAATTGTAAGGCACTGTGTGTGTAATATCATAAACGAATAAAGCTTTTTATTTAGCAAACTAACTANNAAAAAA
TARGET=334,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06354 Ps_contig_mira-and-tgicl-ass_franssen_44560
SEQUENCE=CYCGGGTCGGGCAACAACAAAACCTATTTTGAGAAAACATGGGGTCTCAAATGACCTCTATTACTCTCACTATTGCATTGACAATACTCTCTCTCACCTTGCCATCTCAAGCAAACAATTACCTCTATTCATCTCCACCACCACCACCAAAACCTTACTACTACCAATCTCCACCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCACCGGTTTACAAATCTCCCCCTCCACCATACAAATATCAATCTCCACCACCGCCTCCTTACAAGTACTCATCACCACCTCCACCAGTCTACAAGTACAATTCACCCCCTCCTCCTTACAAGCACATATCTCCTCCAACACCTGGAAAGCCATTCAAGTTCCCACCTCCACCAACACCGATTTACAAATATAAATCTCCTCCACCTGTTTACTCGCCACCACCCGTTTACAAATATAAATCTCCTCCTCCACCTGTTTACTCACCACCTCCACCACACTATGTCTACTCTTCACCTCCTCCACCGGTCTATTCCCCTCCTCCACCTCATTACATATATGCCTCACCTCCTCCTCCTTACCACTACTAGATAGTGGCTTACCGTAGTATCCCATGGATTTGAAGAATATATATAATAATATTGGAAATAAAGAGAACATAGTGCAAAGCAGGAAGATGCATTTCAAAATATACAAGGAAAAAACAACGGAAGGAAAGTTTCCATGCACTTGGTCTCAAGAAAAGTCATTATTCTTTTCCCATTTTGTTTTGTTATGAATTGTTATTCTCATTATTGGAGTGCTTGGTTTTTGAATTTAGACAATTGTAAGGCACTGTGTGTGTAATATCATAAACGAATAAAGCTTTTTATTTAGCAAACTAACTANNAAAAAA
TARGET=382,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06355 Ps_contig_mira-and-tgicl-ass_franssen_44560
SEQUENCE=CYCGGGTCGGGCAACAACAAAACCTATTTTGAGAAAACATGGGGTCTCAAATGACCTCTATTACTCTCACTATTGCATTGACAATACTCTCTCTCACCTTGCCATCTCAAGCAAACAATTACCTCTATTCATCTCCACCACCACCACCAAAACCTTACTACTACCAATCTCCACCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCACCGGTTTACAAATCTCCCCCTCCACCATACAAATATCAATCTCCACCACCGCCTCCTTACAAGTACTCATCACCACCTCCACCAGTCTACAAGTACAATTCACCCCCTCCTCCTTACAAGCACATATCTCCTCCAACACCTGGAAAGCCATTCAAGTTCCCACCTCCACCAACACCGATTTACAAATATAAATCTCCTCCACCTGTTTACTCGCCACCACCCGTTTACAAATATAAATCTCCTCCTCCACCTGTTTACTCACCACCTCCACCACACTATGTCTACTCTTCACCTCCTCCACCGGTCTATTCCCCTCCTCCACCTCATTACATATATGCCTCACCTCCTCCTCCTTACCACTACTAGATAGTGGCTTACCGTAGTATCCCATGGATTTGAAGAATATATATAATAATATTGGAAATAAAGAGAACATAGTGCAAAGCAGGAAGATGCATTTCAAAATATACAAGGAAAAAACAACGGAAGGAAAGTTTCCATGCACTTGGTCTCAAGAAAAGTCATTATTCTTTTCCCATTTTGTTTTGTTATGAATTGTTATTCTCATTATTGGAGTGCTTGGTTTTTGAATTTAGACAATTGTAAGGCACTGTGTGTGTAATATCATAAACGAATAAAGCTTTTTATTTAGCAAACTAACTANNAAAAAA
TARGET=612,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06356 Ps_contig_mira-and-tgicl-ass_franssen_44560
SEQUENCE=CYCGGGTCGGGCAACAACAAAACCTATTTTGAGAAAACATGGGGTCTCAAATGACCTCTATTACTCTCACTATTGCATTGACAATACTCTCTCTCACCTTGCCATCTCAAGCAAACAATTACCTCTATTCATCTCCACCACCACCACCAAAACCTTACTACTACCAATCTCCACCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCACCGGTTTACAAATCTCCCCCTCCACCATACAAATATCAATCTCCACCACCGCCTCCTTACAAGTACTCATCACCACCTCCACCAGTCTACAAGTACAATTCACCCCCTCCTCCTTACAAGCACATATCTCCTCCAACACCTGGAAAGCCATTCAAGTTCCCACCTCCACCAACACCGATTTACAAATATAAATCTCCTCCACCTGTTTACTCGCCACCACCCGTTTACAAATATAAATCTCCTCCTCCACCTGTTTACTCACCACCTCCACCACACTATGTCTACTCTTCACCTCCTCCACCGGTCTATTCCCCTCCTCCACCTCATTACATATATGCCTCACCTCCTCCTCCTTACCACTACTAGATAGTGGCTTACCGTAGTATCCCATGGATTTGAAGAATATATATAATAATATTGGAAATAAAGAGAACATAGTGCAAAGCAGGAAGATGCATTTCAAAATATACAAGGAAAAAACAACGGAAGGAAAGTTTCCATGCACTTGGTCTCAAGAAAAGTCATTATTCTTTTCCCATTTTGTTTTGTTATGAATTGTTATTCTCATTATTGGAGTGCTTGGTTTTTGAATTTAGACAATTGTAAGGCACTGTGTGTGTAATATCATAAACGAATAAAGCTTTTTATTTAGCAAACTAACTANNAAAAAA
TARGET=649,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06357 Ps_contig_mira-and-tgicl-ass_franssen_44560
SEQUENCE=CYCGGGTCGGGCAACAACAAAACCTATTTTGAGAAAACATGGGGTCTCAAATGACCTCTATTACTCTCACTATTGCATTGACAATACTCTCTCTCACCTTGCCATCTCAAGCAAACAATTACCTCTATTCATCTCCACCACCACCACCAAAACCTTACTACTACCAATCTCCACCACCACCGGTGCACTCACCTCCTCCACCTTATCATTATAGTTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAATCTTATAAATATTCTTCTCCTCCACCACCGATTTACAAATATAAGTCACCACCACCACCAGTGCACTCACCTCCTCCACCTTATCACTATAGTTCACCACCACCACCACCAAAGAAGTCGTACAAATATTCTTCTCCTCCACCACCAGTTTACAAGTATAAGTCACCACCTCCACCGGTGTATAAATATAAATCACCACCACCTCCGGTTAAAAAACCATATAAATACTCATCTCCACCACCACCGGTGTACAAGTATAAATCTCCTCCACCACCGGTGTACAAATACAAATCTCCTCCCCCACCTGTATACAAATATCAATCTCCACCACCACCACCTAAGAAATCATACAAATACCCATCACCACCACCACCGGTTTACAAATCTCCCCCTCCACCATACAAATATCAATCTCCACCACCGCCTCCTTACAAGTACTCATCACCACCTCCACCAGTCTACAAGTACAATTCACCCCCTCCTCCTTACAAGCACATATCTCCTCCAACACCTGGAAAGCCATTCAAGTTCCCACCTCCACCAACACCGATTTACAAATATAAATCTCCTCCACCTGTTTACTCGCCACCACCCGTTTACAAATATAAATCTCCTCCTCCACCTGTTTACTCACCACCTCCACCACACTATGTCTACTCTTCACCTCCTCCACCGGTCTATTCCCCTCCTCCACCTCATTACATATATGCCTCACCTCCTCCTCCTTACCACTACTAGATAGTGGCTTACCGTAGTATCCCATGGATTTGAAGAATATATATAATAATATTGGAAATAAAGAGAACATAGTGCAAAGCAGGAAGATGCATTTCAAAATATACAAGGAAAAAACAACGGAAGGAAAGTTTCCATGCACTTGGTCTCAAGAAAAGTCATTATTCTTTTCCCATTTTGTTTTGTTATGAATTGTTATTCTCATTATTGGAGTGCTTGGTTTTTGAATTTAGACAATTGTAAGGCACTGTGTGTGTAATATCATAAACGAATAAAGCTTTTTATTTAGCAAACTAACTANNAAAAAA
TARGET=1002,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06358 Ps_contig_mira-and-tgicl-ass_franssen_44612
SEQUENCE=AAGTAGGTANTACCAACGCAGAGTACTCGGGGACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCACTCTCAACTCCAAAAACATGGAAACAACCCTCCACTCTATCTTTCATCTCTCTCAAACCAACCAAATTCACACCAAGAACAACAAAAATTGCTGCAACAGAAGACAAAACCGATGCAGCAACCGATGTAGCAACAAAAGAAGCACCAGTTGGATTCANCCCACCTGAGCTAGACC
TARGET=37,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.3125
PRIMER_LEFT_PENALTY=2.272667
PRIMER_RIGHT_PENALTY=0.039879
PRIMER_LEFT_SEQUENCE=GCAGAGTACTCGGGGACAAC
PRIMER_RIGHT_SEQUENCE=ATCCAACTGGTGCTTCTTTTGT
PRIMER_LEFT=17,20
PRIMER_RIGHT=244,22
PRIMER_LEFT_TM=59.727
PRIMER_RIGHT_TM=60.040
PRIMER_LEFT_GC_PERCENT=60.000
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=8.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=228
PRIMER_PAIR_PENALTY_1=2.4087
PRIMER_LEFT_1_PENALTY=2.272667
PRIMER_RIGHT_1_PENALTY=0.136017
PRIMER_LEFT_1_SEQUENCE=GCAGAGTACTCGGGGACAAC
PRIMER_RIGHT_1_SEQUENCE=TAGAGTGGAGGGTTGTTTCCAT
PRIMER_LEFT_1=17,20
PRIMER_RIGHT_1=129,22
PRIMER_LEFT_1_TM=59.727
PRIMER_RIGHT_1_TM=59.864
PRIMER_LEFT_1_GC_PERCENT=60.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=113
PRIMER_PAIR_PENALTY_2=2.5615
PRIMER_LEFT_2_PENALTY=2.272667
PRIMER_RIGHT_2_PENALTY=0.288804
PRIMER_LEFT_2_SEQUENCE=GCAGAGTACTCGGGGACAAC
PRIMER_RIGHT_2_SEQUENCE=TGGTTGGTTTGAGAGAGATGAA
PRIMER_LEFT_2=17,20
PRIMER_RIGHT_2=154,22
PRIMER_LEFT_2_TM=59.727
PRIMER_RIGHT_2_TM=59.711
PRIMER_LEFT_2_GC_PERCENT=60.000
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.4000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=138
PRIMER_PAIR_PENALTY_3=2.5615
PRIMER_LEFT_3_PENALTY=2.272667
PRIMER_RIGHT_3_PENALTY=0.288804
PRIMER_LEFT_3_SEQUENCE=GCAGAGTACTCGGGGACAAC
PRIMER_RIGHT_3_SEQUENCE=TTGGTTGGTTTGAGAGAGATGA
PRIMER_LEFT_3=17,20
PRIMER_RIGHT_3=155,22
PRIMER_LEFT_3_TM=59.727
PRIMER_RIGHT_3_TM=59.711
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=139
PRIMER_PAIR_PENALTY_4=2.6078
PRIMER_LEFT_4_PENALTY=2.272667
PRIMER_RIGHT_4_PENALTY=0.335104
PRIMER_LEFT_4_SEQUENCE=GCAGAGTACTCGGGGACAAC
PRIMER_RIGHT_4_SEQUENCE=AACTGGTGCTTCTTTTGTTGCT
PRIMER_LEFT_4=17,20
PRIMER_RIGHT_4=240,22
PRIMER_LEFT_4_TM=59.727
PRIMER_RIGHT_4_TM=60.335
PRIMER_LEFT_4_GC_PERCENT=60.000
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=8.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.4000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=224
=
PRIMER_SEQUENCE_ID=SSR_06359 Ps_contig_mira-and-tgicl-ass_franssen_44668
SEQUENCE=AAAGACAGTCGGGGATATCATAATATCTCTTTTCTTTTCTTTCTTTCATTAAACATTTAGTTTCTTGTCAAAAATGGTGCTAGCTGGTAAGCTTATTACAGAACTTGGTATCAAAACACCAGCTGACAAGTTCTTCAAACTGTTTGCATCAGAACTTCATGAAGTGCAAAACCTTTGTGAAAGAATTCATCATACCAAGATTCATCATGGTGAAGACTGGCATCACTCTGATTCCATTAAACACTGGACTTATGTCATAGATGGGGAGGTACACACATGTAATGAAAGTATTGAAGAAGTAGATGATGAGAACAAAAAGATCACATACAAGCTGTTTGGTGGAGATATTGACAAGTACACGGTTTTTAAGCTGATTCTAGAAGTGATTAATAAGGCTGATGGCACCGCTGCTGTTAAATGGACTATTGATTATGTGAAAATCAATGAAGATGTTGATACTCCAAATGGCTGGATGGATTACCTTAGCAAAAGCACTAGAGACATTGATGGTCATCTTCTCAAGGGAGAAAGAGTTGCACTATAAGTCATAAAGAGCATTGGTTTTGTGGATACACTTCTATATTGCATCTTTATAACTAGTGTGATGATTGCAATGCCATGCATGTATCATATATAAGCATGCATCATGTTTGCTTCTCTTCATGTTTTCTTTGTATGGAATAAATGTACTGGTGATGTATTGAAGATTGTGTTCATGTTTATGTAATAATGTTTCTGATTATAAAAGTTTGGTTTGCCAAATCTTCACAAAAAAATYTCTTCTTACCT
TARGET=28,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06360 Ps_contig_mira-and-tgicl-ass_franssen_44668
SEQUENCE=AAAGACAGTCGGGGATATCATAATATCTCTTTTCTTTTCTTTCTTTCATTAAACATTTAGTTTCTTGTCAAAAATGGTGCTAGCTGGTAAGCTTATTACAGAACTTGGTATCAAAACACCAGCTGACAAGTTCTTCAAACTGTTTGCATCAGAACTTCATGAAGTGCAAAACCTTTGTGAAAGAATTCATCATACCAAGATTCATCATGGTGAAGACTGGCATCACTCTGATTCCATTAAACACTGGACTTATGTCATAGATGGGGAGGTACACACATGTAATGAAAGTATTGAAGAAGTAGATGATGAGAACAAAAAGATCACATACAAGCTGTTTGGTGGAGATATTGACAAGTACACGGTTTTTAAGCTGATTCTAGAAGTGATTAATAAGGCTGATGGCACCGCTGCTGTTAAATGGACTATTGATTATGTGAAAATCAATGAAGATGTTGATACTCCAAATGGCTGGATGGATTACCTTAGCAAAAGCACTAGAGACATTGATGGTCATCTTCTCAAGGGAGAAAGAGTTGCACTATAAGTCATAAAGAGCATTGGTTTTGTGGATACACTTCTATATTGCATCTTTATAACTAGTGTGATGATTGCAATGCCATGCATGTATCATATATAAGCATGCATCATGTTTGCTTCTCTTCATGTTTTCTTTGTATGGAATAAATGTACTGGTGATGTATTGAAGATTGTGTTCATGTTTATGTAATAATGTTTCTGATTATAAAAGTTTGGTTTGCCAAATCTTCACAAAAAAATYTCTTCTTACCT
TARGET=36,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06361 Ps_contig_mira-and-tgicl-ass_franssen_44711
SEQUENCE=TTATGTATTTATTACTAGAAATCCANTTTTTTTTTTTAAAAGACATAATGAAATATCCATTTATTGATTTGCATCACATGATTTAAGTCACACTCTTGAACCGATCACACAGAGAAACGAAAACAGAAAGCAAATATTACAAGCATCACACTAATTAATTATAAGATGATCAGATAAGCCAACTCCATCCATAAACCATGCCATCTTATTTGTTACTTTCTTTTTAATCTTTTCGAGCGGCCCTTGCCTTGGAAAGATGCGCATCAACATCTCTAGTGCATTGGCTAATGTAGTCCATCCATCCATTTGGAGGATGAATATCCTCGTTGATTTTCTCAAATTCAACGGTCCATTTAGCAACAGCAGTGCCATCTTCAGCCTTATCAATTACTTGAAGGGTGAGCTTAAAGGACTTATAGTGCTTATCAATATCTCCACCAAATAGCTTCCAAGTGTTTTTTTTGTTCTGTTCATCAACTTCTTCAAGAGTATCGTAGCATGTGTGTACCTCACCATCTATGACATAAGTCCAGTGTTTAACTGTAGCAGTGTCATGCCAGTCTTCACCTTCATGCAGTTTGGTATGATGAACTCTTTCACAAATGACTTGCGCTTCGTGAAGTTCTGCCGCGAATAGTTTATAGAACTTTTCAGCGGGTGTTTTGATACCAAGTTCAGTAATAAGCTTACCAGAGAGCACCATTTTTTAGGAATAAAATAATTGATCCCCGAGTACTGTATGCAACCCCATGCCTTAAATAC
TARGET=294,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3124
PRIMER_LEFT_PENALTY=0.278329
PRIMER_RIGHT_PENALTY=0.034102
PRIMER_LEFT_SEQUENCE=TCAACATCTCTAGTGCATTGGC
PRIMER_RIGHT_SEQUENCE=TTGGAAGCTATTTGGTGGAGAT
PRIMER_LEFT=263,22
PRIMER_RIGHT=451,22
PRIMER_LEFT_TM=60.278
PRIMER_RIGHT_TM=59.966
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=10.0000
PRIMER_RIGHT_END_STABILITY=6.3000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=189
PRIMER_PAIR_PENALTY_1=0.3574
PRIMER_LEFT_1_PENALTY=0.278329
PRIMER_RIGHT_1_PENALTY=0.079056
PRIMER_LEFT_1_SEQUENCE=TCAACATCTCTAGTGCATTGGC
PRIMER_RIGHT_1_SEQUENCE=GATGGTGAGGTACACACATGCT
PRIMER_LEFT_1=263,22
PRIMER_RIGHT_1=516,22
PRIMER_LEFT_1_TM=60.278
PRIMER_RIGHT_1_TM=59.921
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=10.0000
PRIMER_RIGHT_1_END_STABILITY=8.1000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=254
PRIMER_PAIR_PENALTY_2=0.3574
PRIMER_LEFT_2_PENALTY=0.278329
PRIMER_RIGHT_2_PENALTY=0.079056
PRIMER_LEFT_2_SEQUENCE=TCAACATCTCTAGTGCATTGGC
PRIMER_RIGHT_2_SEQUENCE=AGATGGTGAGGTACACACATGC
PRIMER_LEFT_2=263,22
PRIMER_RIGHT_2=517,22
PRIMER_LEFT_2_TM=60.278
PRIMER_RIGHT_2_TM=59.921
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=10.0000
PRIMER_RIGHT_2_END_STABILITY=8.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=255
PRIMER_PAIR_PENALTY_3=0.4752
PRIMER_LEFT_3_PENALTY=0.231272
PRIMER_RIGHT_3_PENALTY=0.243965
PRIMER_LEFT_3_SEQUENCE=ACTCTTGAACCGATCACACAGA
PRIMER_RIGHT_3_SEQUENCE=GTTGCTAAATGGACCGTTGAAT
PRIMER_LEFT_3=91,22
PRIMER_RIGHT_3=360,22
PRIMER_LEFT_3_TM=59.769
PRIMER_RIGHT_3_TM=60.244
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.4000
PRIMER_RIGHT_3_END_STABILITY=6.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=270
PRIMER_PAIR_PENALTY_4=0.5160
PRIMER_LEFT_4_PENALTY=0.278329
PRIMER_RIGHT_4_PENALTY=0.237699
PRIMER_LEFT_4_SEQUENCE=TCAACATCTCTAGTGCATTGGC
PRIMER_RIGHT_4_SEQUENCE=GTCCTTTAAGCTCACCCTTCAA
PRIMER_LEFT_4=263,22
PRIMER_RIGHT_4=412,22
PRIMER_LEFT_4_TM=60.278
PRIMER_RIGHT_4_TM=59.762
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=10.0000
PRIMER_RIGHT_4_END_STABILITY=7.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_06362 Ps_contig_mira-and-tgicl-ass_franssen_44736
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATCTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTTCTTTTTTTACTGTGTACACTT
TARGET=157,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06363 Ps_contig_mira-and-tgicl-ass_franssen_44737
SEQUENCE=CCTATGTTTGGCACCACTGATGCATCTCAAGTGTTGAAGGAGCTTGAAGAAGCTAAAACTGCATACCCTAATGCATTCATCAGAATCATTGGATTCGACAATGTTCGTCAAGTGCAATGCATTAGTTTCATTGCTCACACACCAGAACCAAAACCTTCAACCAACCACCGTGTCCACCTCCACCATGGTAACCACCACCACCATTATGGTAACCTCCACCGCCATTGTAACCACCTCCACCATTATG
TARGET=191,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4281
PRIMER_LEFT_PENALTY=0.037339
PRIMER_RIGHT_PENALTY=0.390771
PRIMER_LEFT_SEQUENCE=ATTCGACAATGTTCGTCAAGTG
PRIMER_RIGHT_SEQUENCE=CATAATGGTGGAGGTGGTTACA
PRIMER_LEFT=92,22
PRIMER_RIGHT=246,22
PRIMER_LEFT_TM=60.037
PRIMER_RIGHT_TM=59.609
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=155
PRIMER_PAIR_PENALTY_1=0.5695
PRIMER_LEFT_1_PENALTY=0.178718
PRIMER_RIGHT_1_PENALTY=0.390771
PRIMER_LEFT_1_SEQUENCE=CCACTGATGCATCTCAAGTGTT
PRIMER_RIGHT_1_SEQUENCE=CATAATGGTGGAGGTGGTTACA
PRIMER_LEFT_1=13,22
PRIMER_RIGHT_1=246,22
PRIMER_LEFT_1_TM=60.179
PRIMER_RIGHT_1_TM=59.609
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.4000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=234
PRIMER_PAIR_PENALTY_2=0.5844
PRIMER_LEFT_2_PENALTY=0.193666
PRIMER_RIGHT_2_PENALTY=0.390771
PRIMER_LEFT_2_SEQUENCE=CGTCAAGTGCAATGCATTAGTT
PRIMER_RIGHT_2_SEQUENCE=CATAATGGTGGAGGTGGTTACA
PRIMER_LEFT_2=105,22
PRIMER_RIGHT_2=246,22
PRIMER_LEFT_2_TM=60.194
PRIMER_RIGHT_2_TM=59.609
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.7000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=142
PRIMER_PAIR_PENALTY_3=0.6060
PRIMER_LEFT_3_PENALTY=0.215254
PRIMER_RIGHT_3_PENALTY=0.390771
PRIMER_LEFT_3_SEQUENCE=AGTTTCATTGCTCACACACCAG
PRIMER_RIGHT_3_SEQUENCE=CATAATGGTGGAGGTGGTTACA
PRIMER_LEFT_3=123,22
PRIMER_RIGHT_3=246,22
PRIMER_LEFT_3_TM=60.215
PRIMER_RIGHT_3_TM=59.609
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=7.9000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=124
PRIMER_PAIR_PENALTY_4=0.6505
PRIMER_LEFT_4_PENALTY=0.259704
PRIMER_RIGHT_4_PENALTY=0.390771
PRIMER_LEFT_4_SEQUENCE=ATGCATCTCAAGTGTTGAAGGA
PRIMER_RIGHT_4_SEQUENCE=CATAATGGTGGAGGTGGTTACA
PRIMER_LEFT_4=19,22
PRIMER_RIGHT_4=246,22
PRIMER_LEFT_4_TM=59.740
PRIMER_RIGHT_4_TM=59.609
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=228
=
PRIMER_SEQUENCE_ID=SSR_06364 Ps_contig_mira-and-tgicl-ass_franssen_44764
SEQUENCE=CGTTCCACTGATTCAACTGTTTTGTTCTCAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTTGCTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTTCACTTCTTCAACCGATTCCTCAGTTTCTTTAGGTGCTTCTAAGTTCTCTTCCTTTGCTTCCTCTGTTACCACTTCCTTGTTCTCAACTTCAAGTGGATCTTGGGTTTCGGGAGCATCTACAGCTTCTCCTGCTGCAGTTTGTTTCTGGTTTGGTTTTTCGGTTGTTTCTTCTGTTGGTACTTCGGTGGCAGGAACTTCGGATTCTGGTTGTGCCGCAGCGAGGGACTTCAGGTGGCTGGTTGCTTGTTCTTGTAATTTCGTTTCCTCTGTTCTTGTATTATCGTTCGGGTTGTTTGTTTCTTATTTTTCNTGCCTACGTTGGTTTGGTTGGGTGGTGTCGTTGGGTGTGACCTAACCCTAC
TARGET=51,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.2776
PRIMER_LEFT_PENALTY=0.262854
PRIMER_RIGHT_PENALTY=0.014720
PRIMER_LEFT_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_RIGHT_SEQUENCE=CAGAAGAAACAACCGAAAAACC
PRIMER_LEFT=22,22
PRIMER_RIGHT=294,22
PRIMER_LEFT_TM=60.263
PRIMER_RIGHT_TM=60.015
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=8.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=273
PRIMER_PAIR_PENALTY_1=0.3724
PRIMER_LEFT_1_PENALTY=0.262854
PRIMER_RIGHT_1_PENALTY=0.109510
PRIMER_LEFT_1_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_RIGHT_1_SEQUENCE=GGAATCGGTTGAAGAAGTGAAG
PRIMER_LEFT_1=22,22
PRIMER_RIGHT_1=140,22
PRIMER_LEFT_1_TM=60.263
PRIMER_RIGHT_1_TM=60.110
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=119
PRIMER_PAIR_PENALTY_2=0.6140
PRIMER_LEFT_2_PENALTY=0.262854
PRIMER_RIGHT_2_PENALTY=0.351175
PRIMER_LEFT_2_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_RIGHT_2_SEQUENCE=TACCAACAGAAGAAACAACCGA
PRIMER_LEFT_2=22,22
PRIMER_RIGHT_2=300,22
PRIMER_LEFT_2_TM=60.263
PRIMER_RIGHT_2_TM=59.649
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=9.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.6451
PRIMER_LEFT_3_PENALTY=0.642812
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=CTCAATCACATCCTCTGCAGTT
PRIMER_RIGHT_3_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_LEFT_3=26,22
PRIMER_RIGHT_3=132,22
PRIMER_LEFT_3_TM=59.357
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=0.6575
PRIMER_LEFT_4_PENALTY=0.642812
PRIMER_RIGHT_4_PENALTY=0.014720
PRIMER_LEFT_4_SEQUENCE=CTCAATCACATCCTCTGCAGTT
PRIMER_RIGHT_4_SEQUENCE=CAGAAGAAACAACCGAAAAACC
PRIMER_LEFT_4=26,22
PRIMER_RIGHT_4=294,22
PRIMER_LEFT_4_TM=59.357
PRIMER_RIGHT_4_TM=60.015
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=269
=
PRIMER_SEQUENCE_ID=SSR_06365 Ps_contig_mira-and-tgicl-ass_franssen_44773
SEQUENCE=TTGCTTAAGTCAAATGATCCTGTCGGGTTTAACCTTTTGTTTGTATGTTTTGGCTTGGGGTTCTATAGGTCATATTGTTGCTTATTATATCTTGGCCACTTCTAGCAATGGTTATGATCCTAGTTTCTTTGGTTAATTAAGATTACTATGTTGTTCGTTTATGTTGTTTTAATTTGTCTTTTCTTGTCCCTTTTATGTTTATTAAGNTGATGATGATGATGATGAGTGATGTTATTAGTTTTATTTGTATGAGTATATACAACTAAAGTTTAACGTTTCCGTTAAG
TARGET=208,18
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06366 Ps_contig_mira-and-tgicl-ass_franssen_44783
SEQUENCE=GTGACTCTTAAGTTAGCTGAATTTGTGTAAATTANTATATTATTCATGAAGTTGTGAATGTATCTTCTGGTTATGGCAAAAACTACCTCTAAGTAGGATCAAGAGACTGCCACGATCATGTYTATTTAAGTAGTTGGTTATGTCTTGCTGTTACTGTGGTGATGTTTATCCTTAGCCATGGCAGCATAGCAAGGTAAATGAACTAGGAAAATTAATTAATTAAACTTTTATTTATTTTATGTAGTTTATAGGAACAACATNATARNARTATTATTATTCTTATTTAGGTTATTATTGATCAAATAAAACT
TARGET=210,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06367 Ps_contig_mira-and-tgicl-ass_franssen_44837
SEQUENCE=GTTCGGGGGTTGTTCTGCGCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCGGACCTTTGCCGGAGAATGGTCTCGGCAAGCTCCGAAACNCCCTTCCTTCCTTCCCGATCTATCTCCGACAAGGAATCAGGAGGGTGGAAACGTGAGATCCGTACGGGTGGGGATTCGGTTAGGAGGGTGCAGTGAAAGTGACACGTAGTATCATGATTAGGTACGAAACCAGGTCCGG
TARGET=114,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3198
PRIMER_LEFT_PENALTY=0.231280
PRIMER_RIGHT_PENALTY=0.088563
PRIMER_LEFT_SEQUENCE=GTTCTGCGCTTTCCATATTCTC
PRIMER_RIGHT_SEQUENCE=CCTGATTCCTTGTCGGAGATAG
PRIMER_LEFT=11,22
PRIMER_RIGHT=152,22
PRIMER_LEFT_TM=60.231
PRIMER_RIGHT_TM=60.089
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=5.6000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=142
PRIMER_PAIR_PENALTY_1=0.3643
PRIMER_LEFT_1_PENALTY=0.275745
PRIMER_RIGHT_1_PENALTY=0.088563
PRIMER_LEFT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_1_SEQUENCE=CCTGATTCCTTGTCGGAGATAG
PRIMER_LEFT_1=33,22
PRIMER_RIGHT_1=152,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=60.089
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=5.6000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=120
PRIMER_PAIR_PENALTY_2=0.5490
PRIMER_LEFT_2_PENALTY=0.231280
PRIMER_RIGHT_2_PENALTY=0.317768
PRIMER_LEFT_2_SEQUENCE=GTTCTGCGCTTTCCATATTCTC
PRIMER_RIGHT_2_SEQUENCE=ACTTTCACTGCACCCTCCTAAC
PRIMER_LEFT_2=11,22
PRIMER_RIGHT_2=211,22
PRIMER_LEFT_2_TM=60.231
PRIMER_RIGHT_2_TM=59.682
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.7000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=201
PRIMER_PAIR_PENALTY_3=0.5860
PRIMER_LEFT_3_PENALTY=0.497481
PRIMER_RIGHT_3_PENALTY=0.088563
PRIMER_LEFT_3_SEQUENCE=GTTGTTCTGCGCTTTCCATATT
PRIMER_RIGHT_3_SEQUENCE=CCTGATTCCTTGTCGGAGATAG
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=152,22
PRIMER_LEFT_3_TM=60.497
PRIMER_RIGHT_3_TM=60.089
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=5.6000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=145
PRIMER_PAIR_PENALTY_4=0.5935
PRIMER_LEFT_4_PENALTY=0.275745
PRIMER_RIGHT_4_PENALTY=0.317768
PRIMER_LEFT_4_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_4_SEQUENCE=ACTTTCACTGCACCCTCCTAAC
PRIMER_LEFT_4=33,22
PRIMER_RIGHT_4=211,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=59.682
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.7000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=179
=
PRIMER_SEQUENCE_ID=SSR_06368 Ps_contig_mira-and-tgicl-ass_franssen_44902
SEQUENCE=AAATTCAATAACATTGGTTGAATTTTTCAAGAACTAATAGTAATAAAACAAAATATACATGCATAAATATCATGATCCGATGCTTCTGCTTCACAAACAAAATACATTCCACAACAATTTTTTGACACAATTTTTTCTTTTCAATCTAAAAAATATAATAAACCCAAACAAATTTTTCTTTTATGTGTATTTAAAAAAGTACCCACAAAGACAAGTTAAGAACCAAATACATATACCATTAATTCAAGAAAGAAATCACCACACAAGGCCTTAATGATAGTAATTAATTAAACAAAACACCCCCAAAGCCACAAACCAACACAACCAATCGATCATATTCAATGAGAAGTAGTCTCATCCTTGTGATGATCTTTGTCTTCACCATCAGTAGCAGTCTTAGCATGATAACCAGGAAGCTTCTCTTTGATCTTTTCCAAAATACCTTTCTTCTCTCCCTCATGAATAGTTGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTTGTTACTTCCTCTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTAATTTTGTCAAGGAAACCCTTCTTTTCCTCGGTACCGGTTGTTCCTTCAACAACCTCAACTTTCTCAACTGGCACCGAAGTGTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTTCTCACCATCTTCTCCTTCCTCTTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGGTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCACCTTGTGACCAAACTCGTGGGCAATCGCCTCATCTTGAGCAGGCTTATGTTCTTCATCCTTTTTCTTACCACCGAGAAAATCAAAAACACCTCTGTCCTTGATCTCTGTCTCAGAGTTGGTTGCGGAGGTAGTCTCCTCGTACTTGTTCTGATTCTCCTCAGCCATGATGATCACTACTAACAAGATGGATCGAACTGGAAATTAATTAACTGTAACTAAAATGGATTATGTAGCTGTAACTCTGAAATGAAAGGTAAAGTATGAGACGATGATGATAGTGTTGTTGTTGTTTGGATCATCCCCGACTGAGAAGG
TARGET=672,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0895
PRIMER_LEFT_PENALTY=0.071684
PRIMER_RIGHT_PENALTY=0.017846
PRIMER_LEFT_SEQUENCE=GCACCGAAGTGTCTTCTTTCTT
PRIMER_RIGHT_SEQUENCE=TGAAGAACATAAGCCTGCTCAA
PRIMER_LEFT=640,22
PRIMER_RIGHT=881,22
PRIMER_LEFT_TM=59.928
PRIMER_RIGHT_TM=60.018
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=242
PRIMER_PAIR_PENALTY_1=0.0915
PRIMER_LEFT_1_PENALTY=0.071684
PRIMER_RIGHT_1_PENALTY=0.019805
PRIMER_LEFT_1_SEQUENCE=GCACCGAAGTGTCTTCTTTCTT
PRIMER_RIGHT_1_SEQUENCE=GAGGTGTTTTTGATTTTCTCGG
PRIMER_LEFT_1=640,22
PRIMER_RIGHT_1=918,22
PRIMER_LEFT_1_TM=59.928
PRIMER_RIGHT_1_TM=59.980
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=9.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=279
PRIMER_PAIR_PENALTY_2=0.1891
PRIMER_LEFT_2_PENALTY=0.071684
PRIMER_RIGHT_2_PENALTY=0.117452
PRIMER_LEFT_2_SEQUENCE=GCACCGAAGTGTCTTCTTTCTT
PRIMER_RIGHT_2_SEQUENCE=GGAGAAACTTCACCGATCTGAC
PRIMER_LEFT_2=640,22
PRIMER_RIGHT_2=755,22
PRIMER_LEFT_2_TM=59.928
PRIMER_RIGHT_2_TM=60.117
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.4000
PRIMER_PAIR_2_COMPL_ANY=7.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=116
PRIMER_PAIR_PENALTY_3=0.2033
PRIMER_LEFT_3_PENALTY=0.088876
PRIMER_RIGHT_3_PENALTY=0.114470
PRIMER_LEFT_3_SEQUENCE=CGGGAAGCTTCTCCTTAATTTT
PRIMER_RIGHT_3_SEQUENCE=GGAAGGTGAAAAGAAACACACC
PRIMER_LEFT_3=553,22
PRIMER_RIGHT_3=785,22
PRIMER_LEFT_3_TM=60.089
PRIMER_RIGHT_3_TM=59.886
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=8.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=7.2000
PRIMER_RIGHT_3_END_STABILITY=7.6000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=233
PRIMER_PAIR_PENALTY_4=0.2109
PRIMER_LEFT_4_PENALTY=0.205226
PRIMER_RIGHT_4_PENALTY=0.005674
PRIMER_LEFT_4_SEQUENCE=AACCTCAACTTTCTCAACTGGC
PRIMER_RIGHT_4_SEQUENCE=CTCGGTGGTAAGAAAAAGGATG
PRIMER_LEFT_4=620,22
PRIMER_RIGHT_4=901,22
PRIMER_LEFT_4_TM=59.795
PRIMER_RIGHT_4_TM=59.994
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=3.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.1000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=282
=
PRIMER_SEQUENCE_ID=SSR_06369 Ps_contig_mira-and-tgicl-ass_franssen_44902
SEQUENCE=AAATTCAATAACATTGGTTGAATTTTTCAAGAACTAATAGTAATAAAACAAAATATACATGCATAAATATCATGATCCGATGCTTCTGCTTCACAAACAAAATACATTCCACAACAATTTTTTGACACAATTTTTTCTTTTCAATCTAAAAAATATAATAAACCCAAACAAATTTTTCTTTTATGTGTATTTAAAAAAGTACCCACAAAGACAAGTTAAGAACCAAATACATATACCATTAATTCAAGAAAGAAATCACCACACAAGGCCTTAATGATAGTAATTAATTAAACAAAACACCCCCAAAGCCACAAACCAACACAACCAATCGATCATATTCAATGAGAAGTAGTCTCATCCTTGTGATGATCTTTGTCTTCACCATCAGTAGCAGTCTTAGCATGATAACCAGGAAGCTTCTCTTTGATCTTTTCCAAAATACCTTTCTTCTCTCCCTCATGAATAGTTGTTGTTGTAGCTGTTGTCTCAGTTGGAACATGAGCAGCGACAACTGGTGGAGGAGTTGTTACTTCCTCTGTTTTCTTGTGTCCTCCGGGAAGCTTCTCCTTAATTTTGTCAAGGAAACCCTTCTTTTCCTCGGTACCGGTTGTTCCTTCAACAACCTCAACTTTCTCAACTGGCACCGAAGTGTCTTCTTTCTTCTTCTTTTCCTTCTTCTTCTTTCTTTTCTCACCATCTTCTCCTTCCTCTTCACTTGAGGAGCTTGATGAGCTGTCAGATCGGTGAAGTTTCTCCAAGAGACTGGTGTGTTTCTTTTCACCTTCCTCTTCAACTTTGGTTTCCGACGGTGCTTCATACAAAGTCACCTTGTGACCAAACTCGTGGGCAATCGCCTCATCTTGAGCAGGCTTATGTTCTTCATCCTTTTTCTTACCACCGAGAAAATCAAAAACACCTCTGTCCTTGATCTCTGTCTCAGAGTTGGTTGCGGAGGTAGTCTCCTCGTACTTGTTCTGATTCTCCTCAGCCATGATGATCACTACTAACAAGATGGATCGAACTGGAAATTAATTAACTGTAACTAAAATGGATTATGTAGCTGTAACTCTGAAATGAAAGGTAAAGTATGAGACGATGATGATAGTGTTGTTGTTGTTTGGATCATCCCCGACTGAGAAGG
TARGET=1106,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=3.3966
PRIMER_LEFT_PENALTY=0.005674
PRIMER_RIGHT_PENALTY=3.390877
PRIMER_LEFT_SEQUENCE=CATCCTTTTTCTTACCACCGAG
PRIMER_RIGHT_SEQUENCE=CCTTCTCAGTCGGGGATGAT
PRIMER_LEFT=880,22
PRIMER_RIGHT=1140,20
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=61.391
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=9.9000
PRIMER_RIGHT_END_STABILITY=6.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=261
PRIMER_PAIR_PENALTY_1=3.4087
PRIMER_LEFT_1_PENALTY=0.017846
PRIMER_RIGHT_1_PENALTY=3.390877
PRIMER_LEFT_1_SEQUENCE=TTGAGCAGGCTTATGTTCTTCA
PRIMER_RIGHT_1_SEQUENCE=CCTTCTCAGTCGGGGATGAT
PRIMER_LEFT_1=860,22
PRIMER_RIGHT_1=1140,20
PRIMER_LEFT_1_TM=60.018
PRIMER_RIGHT_1_TM=61.391
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=7.0000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=281
PRIMER_PAIR_PENALTY_2=3.4107
PRIMER_LEFT_2_PENALTY=0.019805
PRIMER_RIGHT_2_PENALTY=3.390877
PRIMER_LEFT_2_SEQUENCE=CCGAGAAAATCAAAAACACCTC
PRIMER_RIGHT_2_SEQUENCE=CCTTCTCAGTCGGGGATGAT
PRIMER_LEFT_2=897,22
PRIMER_RIGHT_2=1140,20
PRIMER_LEFT_2_TM=59.980
PRIMER_RIGHT_2_TM=61.391
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=6.5000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=244
PRIMER_PAIR_PENALTY_3=3.7105
PRIMER_LEFT_3_PENALTY=0.319597
PRIMER_RIGHT_3_PENALTY=3.390877
PRIMER_LEFT_3_SEQUENCE=CAAGATGGATCGAACTGGAAAT
PRIMER_RIGHT_3_SEQUENCE=CCTTCTCAGTCGGGGATGAT
PRIMER_LEFT_3=1007,22
PRIMER_RIGHT_3=1140,20
PRIMER_LEFT_3_TM=60.320
PRIMER_RIGHT_3_TM=61.391
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=134
PRIMER_PAIR_PENALTY_4=3.7273
PRIMER_LEFT_4_PENALTY=0.336392
PRIMER_RIGHT_4_PENALTY=3.390877
PRIMER_LEFT_4_SEQUENCE=TTCATCCTTTTTCTTACCACCG
PRIMER_RIGHT_4_SEQUENCE=CCTTCTCAGTCGGGGATGAT
PRIMER_LEFT_4=878,22
PRIMER_RIGHT_4=1140,20
PRIMER_LEFT_4_TM=60.336
PRIMER_RIGHT_4_TM=61.391
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.9000
PRIMER_RIGHT_4_END_STABILITY=6.5000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=263
=
PRIMER_SEQUENCE_ID=SSR_06370 Ps_contig_mira-and-tgicl-ass_franssen_44912
SEQUENCE=TTCGGGTATTCAACACAAACCTAAGCATTTCAACTATTTATATATATATTCACAGAGATCATATCGTATATCATAAGATGACTGGTTTATTCAGCAACATCTTTTGGTGGGTACATGGTGGAAACAATGGAACTGTTGATGCATTTAACAACAGTGTGCTATCAAGATTATGGTGAGGCTNACATTACAAAACGTGGTGCAACTAAATTACCATGGTA
TARGET=39,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.6581
PRIMER_LEFT_PENALTY=0.620602
PRIMER_RIGHT_PENALTY=0.037486
PRIMER_LEFT_SEQUENCE=TCGGGTATTCAACACAAACCTA
PRIMER_RIGHT_SEQUENCE=AGTTGCACCACGTTTTGTAATG
PRIMER_LEFT=1,22
PRIMER_RIGHT=203,22
PRIMER_LEFT_TM=59.379
PRIMER_RIGHT_TM=59.963
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=6.2000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=203
PRIMER_PAIR_PENALTY_1=0.7453
PRIMER_LEFT_1_PENALTY=0.620602
PRIMER_RIGHT_1_PENALTY=0.124655
PRIMER_LEFT_1_SEQUENCE=TCGGGTATTCAACACAAACCTA
PRIMER_RIGHT_1_SEQUENCE=TCAACAGTTCCATTGTTTCCAC
PRIMER_LEFT_1=1,22
PRIMER_RIGHT_1=138,22
PRIMER_LEFT_1_TM=59.379
PRIMER_RIGHT_1_TM=59.875
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.9000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=0.7474
PRIMER_LEFT_2_PENALTY=0.622772
PRIMER_RIGHT_2_PENALTY=0.124655
PRIMER_LEFT_2_SEQUENCE=TTCGGGTATTCAACACAAACCT
PRIMER_RIGHT_2_SEQUENCE=TCAACAGTTCCATTGTTTCCAC
PRIMER_LEFT_2=0,22
PRIMER_RIGHT_2=138,22
PRIMER_LEFT_2_TM=60.623
PRIMER_RIGHT_2_TM=59.875
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=139
PRIMER_PAIR_PENALTY_3=0.7861
PRIMER_LEFT_3_PENALTY=0.620602
PRIMER_RIGHT_3_PENALTY=0.165486
PRIMER_LEFT_3_SEQUENCE=TCGGGTATTCAACACAAACCTA
PRIMER_RIGHT_3_SEQUENCE=GTTGCACCACGTTTTGTAATGT
PRIMER_LEFT_3=1,22
PRIMER_RIGHT_3=202,22
PRIMER_LEFT_3_TM=59.379
PRIMER_RIGHT_3_TM=59.835
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=202
PRIMER_PAIR_PENALTY_4=0.7883
PRIMER_LEFT_4_PENALTY=0.622772
PRIMER_RIGHT_4_PENALTY=0.165486
PRIMER_LEFT_4_SEQUENCE=TTCGGGTATTCAACACAAACCT
PRIMER_RIGHT_4_SEQUENCE=GTTGCACCACGTTTTGTAATGT
PRIMER_LEFT_4=0,22
PRIMER_RIGHT_4=202,22
PRIMER_LEFT_4_TM=60.623
PRIMER_RIGHT_4_TM=59.835
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=6.6000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=203
=
PRIMER_SEQUENCE_ID=SSR_06371 Ps_contig_mira-and-tgicl-ass_franssen_44931
SEQUENCE=GATTCCCTTTTCCTATTNNNRRRTATCGAAGTTTATCGCTCCCAACCCCAAACACGCTTTCTTTTCTCTTCTCCGTTAGGGTTTTCTCCACCCTCAGGGTTAGGGTTATATTCTCCCTTTTCTTTCCCCARATCRATCCAAACAACAACAACACTATCATCATCGTCTCATACTTTACCTTTCATTTCAGAGTTACAGCTACATAATCCATTTTAGTTACAGTTAATTAATTTCCAGTTCGATCCATCTTGTTAGTAGTGATCATCATGGCTGAGGAGAATCAGAACAAGTACGAGGACACCTACCCTCCGACAAACCYAACTCGTRGRAAGACAGGAGATCAAAGGGACAGGTAGGGTTTNTTTTTTGTA
TARGET=141,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06372 Ps_contig_mira-and-tgicl-ass_franssen_44981
SEQUENCE=TGTTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACNGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTATTATGTGTTGTGAGTTTCTTTTTTTACTGTNTACNCTTCCA
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06373 Ps_contig_mira-and-tgicl-ass_franssen_45024
SEQUENCE=GGGCCGGATAACAAATAGCGCCGTCGCAATGGGAAGAAGACCTGCAAGATGTTACCGTCAAATTAAAAACAAGCCATATCCAAAGTCACGATTNTGTCGTGGTGTTCCTGATCCCAAGATCAGGATCTACGATGTTGGCNTGAAGAAGAAGGGTGTTGAGNGTTCCCTTTCTGTGTCCATCTTGTCAGTTGGGGAGGAAGGAAGGAATTGTTTCAAGTGAGG
TARGET=195,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06374 Ps_contig_mira-and-tgicl-ass_franssen_45062
SEQUENCE=AAGGGRCGTRGGTAGTCAACGACAGAGTCGGGGACACAAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGACCCTTTAACCAAAGAAGAGTTTCTAGCTGTTCAAACCATAGTCCAAAAACAAATACCCCATCTCAAACAACAAACTAGCTTTCCACTATATTGGCCTAGATGACCCAGAAAAAGATCATGTCCTAAGATATGAAACACACCCCACACTTGTATCAATCCCTCGTAAAAGCTTTGTTGTTGCTATCATCAATAGCCAAACCCATGAGATATTAATTAATTTAAGGATCAGAAGCATTGTCTCTGATAATATTCATAATGGATATGGTTTCCCTATCTTATCCGTTGATGAACAATCCCTAGCAATAAAGCTTCCACTAAAATATCCTCCTTTTATTGATTCAGTTAAAAAAAGAGGATTGAATCTCTCAGAGATAGTGTGTTCTAGTTTCACTATGGGCTGGTTTGGAGAAGAGAAGAATGTTAGAACAGTGAGATTAGATTGTTTCATGAAAGAAAGCACTGTGAATATCTATGTGAGACCTATTACTGGAATTACAATAGTTGCTGATCTTGATCTTATGAAAATAGTTGAGTATCATGACAGAGATATTGAAGCAGTACCAACCGCAGAGAACACAGAATATCAAGTTTCAAAACAAAGCCCACCATTTGGACCAAAACAACACAGTCTCACCAGTCATCAGCCACAAGGTCCAGGATTCCAGATCAAAGGCCATAGTGTTAGTTGGGCAAACTGGAAGTTTCACATAGGATTTGATGTACGAGCTGGTATCGTTATATCACTTGCTTCTATTTACGATCTTGAAAAGCATAAGTCTCGTCGTGTACTATACAAAGGCTACATTTCTGAACTTTTTGTACCTTATCAAGACCCTACAGAAGAATTTTACTTTAAAACCTTCTTTGATTCTGGAGAGTTTGGATTTGGTTTATCTACCGTGTCTTTGATACCTAACCGCGATTGTCCACCACATGCTCAATTCATTGACACATACATTCACTCAGCTAATGGTACCCCAATCTTGTTGAAGAACGCAATTTGTGTTTTTGAACAATATGGAAATATCATGTGGCGTCACACTGAAAATGGAATTCCTAATGAATCCATCGAAGAATCTAGAACCGAAGTGAACTTGATCGTAAGAACCATAGTGACGGTTGGAAACTATGACAATGTTATAGATTGGGAGTTCAAAGCAAGTGGTTCCATCAAGCCTGCGATAGCATTATCTGGTATACTTGAAATTAAGGGAACAAATATTAAGCACAAGGATGAGATTAAGGAAGATCTACATGGTAAACTTGTATCAGCAAACAGTATTGGAATTTACCATGACCATTTCTATATTTACTATCTTGATTTTGATATTGATGGTACACATAACTCATTTGAGAAAACAAGTTTGAAGACAGTAAGAATCAAAGATGGAAGTTCAAAGAGAAAGAGTTATTGGACCACTGAAACTCAAACTGCTAAGACAGAATCAGATGCAAAAATCACAATTGGACTTGCACCTGCTGAACTTGTTGTGGTTAATCCAAACATTAAAACTGCTGTTGGAAACGAAGTTGGTTATCGTTTGATTCCAGCAATTCCAGCTCATCCACTATTAACAGAGGATGATTATCCACAAATACGTGGTGCTTTTACAAACTATAATGTTTGGGTGACAGCATATAATAGAACTGAAAAATGGGCGGGTGGACTTTACGTTGATCATAGTCGCGGTGATGATACTTTGGCAGTTTGGACTAAACAGAATAGAGAGATTGTGAACAAGGACATAGTGATGTGGCATGTAGTTGGAATTCATCATGTTCCAGCACAGGAAGATTTCCCAATAATGCCACTATTGAGCACCTCATTTGAGCTGAGGCCAACCAATTTCTTTGAGAGGAATCCAGTTCTTAAAACACTTTCACCAAGAGATGTTGCATGGCCTGGTTGCTCCAATTAAAACTATCCAACATTCTGTATGGAACTGATTTGCATTGGCCTTGTGGTCATATGAATAAATGTTGTTTGTTTGTTTGTTGCATGTGGAAGAATAATGTATGGGTTTCAACTATTTTATTTGAATGAATGAATGAGAGGTTTATTTTAATAACGACTAGT
TARGET=62,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06375 Ps_contig_mira-and-tgicl-ass_franssen_45062
SEQUENCE=AAGGGRCGTRGGTAGTCAACGACAGAGTCGGGGACACAAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGACCCTTTAACCAAAGAAGAGTTTCTAGCTGTTCAAACCATAGTCCAAAAACAAATACCCCATCTCAAACAACAAACTAGCTTTCCACTATATTGGCCTAGATGACCCAGAAAAAGATCATGTCCTAAGATATGAAACACACCCCACACTTGTATCAATCCCTCGTAAAAGCTTTGTTGTTGCTATCATCAATAGCCAAACCCATGAGATATTAATTAATTTAAGGATCAGAAGCATTGTCTCTGATAATATTCATAATGGATATGGTTTCCCTATCTTATCCGTTGATGAACAATCCCTAGCAATAAAGCTTCCACTAAAATATCCTCCTTTTATTGATTCAGTTAAAAAAAGAGGATTGAATCTCTCAGAGATAGTGTGTTCTAGTTTCACTATGGGCTGGTTTGGAGAAGAGAAGAATGTTAGAACAGTGAGATTAGATTGTTTCATGAAAGAAAGCACTGTGAATATCTATGTGAGACCTATTACTGGAATTACAATAGTTGCTGATCTTGATCTTATGAAAATAGTTGAGTATCATGACAGAGATATTGAAGCAGTACCAACCGCAGAGAACACAGAATATCAAGTTTCAAAACAAAGCCCACCATTTGGACCAAAACAACACAGTCTCACCAGTCATCAGCCACAAGGTCCAGGATTCCAGATCAAAGGCCATAGTGTTAGTTGGGCAAACTGGAAGTTTCACATAGGATTTGATGTACGAGCTGGTATCGTTATATCACTTGCTTCTATTTACGATCTTGAAAAGCATAAGTCTCGTCGTGTACTATACAAAGGCTACATTTCTGAACTTTTTGTACCTTATCAAGACCCTACAGAAGAATTTTACTTTAAAACCTTCTTTGATTCTGGAGAGTTTGGATTTGGTTTATCTACCGTGTCTTTGATACCTAACCGCGATTGTCCACCACATGCTCAATTCATTGACACATACATTCACTCAGCTAATGGTACCCCAATCTTGTTGAAGAACGCAATTTGTGTTTTTGAACAATATGGAAATATCATGTGGCGTCACACTGAAAATGGAATTCCTAATGAATCCATCGAAGAATCTAGAACCGAAGTGAACTTGATCGTAAGAACCATAGTGACGGTTGGAAACTATGACAATGTTATAGATTGGGAGTTCAAAGCAAGTGGTTCCATCAAGCCTGCGATAGCATTATCTGGTATACTTGAAATTAAGGGAACAAATATTAAGCACAAGGATGAGATTAAGGAAGATCTACATGGTAAACTTGTATCAGCAAACAGTATTGGAATTTACCATGACCATTTCTATATTTACTATCTTGATTTTGATATTGATGGTACACATAACTCATTTGAGAAAACAAGTTTGAAGACAGTAAGAATCAAAGATGGAAGTTCAAAGAGAAAGAGTTATTGGACCACTGAAACTCAAACTGCTAAGACAGAATCAGATGCAAAAATCACAATTGGACTTGCACCTGCTGAACTTGTTGTGGTTAATCCAAACATTAAAACTGCTGTTGGAAACGAAGTTGGTTATCGTTTGATTCCAGCAATTCCAGCTCATCCACTATTAACAGAGGATGATTATCCACAAATACGTGGTGCTTTTACAAACTATAATGTTTGGGTGACAGCATATAATAGAACTGAAAAATGGGCGGGTGGACTTTACGTTGATCATAGTCGCGGTGATGATACTTTGGCAGTTTGGACTAAACAGAATAGAGAGATTGTGAACAAGGACATAGTGATGTGGCATGTAGTTGGAATTCATCATGTTCCAGCACAGGAAGATTTCCCAATAATGCCACTATTGAGCACCTCATTTGAGCTGAGGCCAACCAATTTCTTTGAGAGGAATCCAGTTCTTAAAACACTTTCACCAAGAGATGTTGCATGGCCTGGTTGCTCCAATTAAAACTATCCAACATTCTGTATGGAACTGATTTGCATTGGCCTTGTGGTCATATGAATAAATGTTGTTTGTTTGTTTGTTGCATGTGGAAGAATAATGTATGGGTTTCAACTATTTTATTTGAATGAATGAATGAGAGGTTTATTTTAATAACGACTAGT
TARGET=91,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06376 Ps_contig_mira-and-tgicl-ass_franssen_45062
SEQUENCE=AAGGGRCGTRGGTAGTCAACGACAGAGTCGGGGACACAAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGACCCTTTAACCAAAGAAGAGTTTCTAGCTGTTCAAACCATAGTCCAAAAACAAATACCCCATCTCAAACAACAAACTAGCTTTCCACTATATTGGCCTAGATGACCCAGAAAAAGATCATGTCCTAAGATATGAAACACACCCCACACTTGTATCAATCCCTCGTAAAAGCTTTGTTGTTGCTATCATCAATAGCCAAACCCATGAGATATTAATTAATTTAAGGATCAGAAGCATTGTCTCTGATAATATTCATAATGGATATGGTTTCCCTATCTTATCCGTTGATGAACAATCCCTAGCAATAAAGCTTCCACTAAAATATCCTCCTTTTATTGATTCAGTTAAAAAAAGAGGATTGAATCTCTCAGAGATAGTGTGTTCTAGTTTCACTATGGGCTGGTTTGGAGAAGAGAAGAATGTTAGAACAGTGAGATTAGATTGTTTCATGAAAGAAAGCACTGTGAATATCTATGTGAGACCTATTACTGGAATTACAATAGTTGCTGATCTTGATCTTATGAAAATAGTTGAGTATCATGACAGAGATATTGAAGCAGTACCAACCGCAGAGAACACAGAATATCAAGTTTCAAAACAAAGCCCACCATTTGGACCAAAACAACACAGTCTCACCAGTCATCAGCCACAAGGTCCAGGATTCCAGATCAAAGGCCATAGTGTTAGTTGGGCAAACTGGAAGTTTCACATAGGATTTGATGTACGAGCTGGTATCGTTATATCACTTGCTTCTATTTACGATCTTGAAAAGCATAAGTCTCGTCGTGTACTATACAAAGGCTACATTTCTGAACTTTTTGTACCTTATCAAGACCCTACAGAAGAATTTTACTTTAAAACCTTCTTTGATTCTGGAGAGTTTGGATTTGGTTTATCTACCGTGTCTTTGATACCTAACCGCGATTGTCCACCACATGCTCAATTCATTGACACATACATTCACTCAGCTAATGGTACCCCAATCTTGTTGAAGAACGCAATTTGTGTTTTTGAACAATATGGAAATATCATGTGGCGTCACACTGAAAATGGAATTCCTAATGAATCCATCGAAGAATCTAGAACCGAAGTGAACTTGATCGTAAGAACCATAGTGACGGTTGGAAACTATGACAATGTTATAGATTGGGAGTTCAAAGCAAGTGGTTCCATCAAGCCTGCGATAGCATTATCTGGTATACTTGAAATTAAGGGAACAAATATTAAGCACAAGGATGAGATTAAGGAAGATCTACATGGTAAACTTGTATCAGCAAACAGTATTGGAATTTACCATGACCATTTCTATATTTACTATCTTGATTTTGATATTGATGGTACACATAACTCATTTGAGAAAACAAGTTTGAAGACAGTAAGAATCAAAGATGGAAGTTCAAAGAGAAAGAGTTATTGGACCACTGAAACTCAAACTGCTAAGACAGAATCAGATGCAAAAATCACAATTGGACTTGCACCTGCTGAACTTGTTGTGGTTAATCCAAACATTAAAACTGCTGTTGGAAACGAAGTTGGTTATCGTTTGATTCCAGCAATTCCAGCTCATCCACTATTAACAGAGGATGATTATCCACAAATACGTGGTGCTTTTACAAACTATAATGTTTGGGTGACAGCATATAATAGAACTGAAAAATGGGCGGGTGGACTTTACGTTGATCATAGTCGCGGTGATGATACTTTGGCAGTTTGGACTAAACAGAATAGAGAGATTGTGAACAAGGACATAGTGATGTGGCATGTAGTTGGAATTCATCATGTTCCAGCACAGGAAGATTTCCCAATAATGCCACTATTGAGCACCTCATTTGAGCTGAGGCCAACCAATTTCTTTGAGAGGAATCCAGTTCTTAAAACACTTTCACCAAGAGATGTTGCATGGCCTGGTTGCTCCAATTAAAACTATCCAACATTCTGTATGGAACTGATTTGCATTGGCCTTGTGGTCATATGAATAAATGTTGTTTGTTTGTTTGTTGCATGTGGAAGAATAATGTATGGGTTTCAACTATTTTATTTGAATGAATGAATGAGAGGTTTATTTTAATAACGACTAGT
TARGET=2171,16
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06377 Ps_contig_mira-and-tgicl-ass_franssen_45062
SEQUENCE=AAGGGRCGTRGGTAGTCAACGACAGAGTCGGGGACACAAAAAAAACACACCAAGCCAGTGAAGCTAGCTAGCTATAGCTCACCATGGCTTCCACCACCACCATGAAACTTGCTCTTTTTTCTGTTCTTACACTCCTCTCATTTCATGCAGTTGTCTCAGTTACACCGTTGCATGTTCAACATCCACTTGACCCTTTAACCAAAGAAGAGTTTCTAGCTGTTCAAACCATAGTCCAAAAACAAATACCCCATCTCAAACAACAAACTAGCTTTCCACTATATTGGCCTAGATGACCCAGAAAAAGATCATGTCCTAAGATATGAAACACACCCCACACTTGTATCAATCCCTCGTAAAAGCTTTGTTGTTGCTATCATCAATAGCCAAACCCATGAGATATTAATTAATTTAAGGATCAGAAGCATTGTCTCTGATAATATTCATAATGGATATGGTTTCCCTATCTTATCCGTTGATGAACAATCCCTAGCAATAAAGCTTCCACTAAAATATCCTCCTTTTATTGATTCAGTTAAAAAAAGAGGATTGAATCTCTCAGAGATAGTGTGTTCTAGTTTCACTATGGGCTGGTTTGGAGAAGAGAAGAATGTTAGAACAGTGAGATTAGATTGTTTCATGAAAGAAAGCACTGTGAATATCTATGTGAGACCTATTACTGGAATTACAATAGTTGCTGATCTTGATCTTATGAAAATAGTTGAGTATCATGACAGAGATATTGAAGCAGTACCAACCGCAGAGAACACAGAATATCAAGTTTCAAAACAAAGCCCACCATTTGGACCAAAACAACACAGTCTCACCAGTCATCAGCCACAAGGTCCAGGATTCCAGATCAAAGGCCATAGTGTTAGTTGGGCAAACTGGAAGTTTCACATAGGATTTGATGTACGAGCTGGTATCGTTATATCACTTGCTTCTATTTACGATCTTGAAAAGCATAAGTCTCGTCGTGTACTATACAAAGGCTACATTTCTGAACTTTTTGTACCTTATCAAGACCCTACAGAAGAATTTTACTTTAAAACCTTCTTTGATTCTGGAGAGTTTGGATTTGGTTTATCTACCGTGTCTTTGATACCTAACCGCGATTGTCCACCACATGCTCAATTCATTGACACATACATTCACTCAGCTAATGGTACCCCAATCTTGTTGAAGAACGCAATTTGTGTTTTTGAACAATATGGAAATATCATGTGGCGTCACACTGAAAATGGAATTCCTAATGAATCCATCGAAGAATCTAGAACCGAAGTGAACTTGATCGTAAGAACCATAGTGACGGTTGGAAACTATGACAATGTTATAGATTGGGAGTTCAAAGCAAGTGGTTCCATCAAGCCTGCGATAGCATTATCTGGTATACTTGAAATTAAGGGAACAAATATTAAGCACAAGGATGAGATTAAGGAAGATCTACATGGTAAACTTGTATCAGCAAACAGTATTGGAATTTACCATGACCATTTCTATATTTACTATCTTGATTTTGATATTGATGGTACACATAACTCATTTGAGAAAACAAGTTTGAAGACAGTAAGAATCAAAGATGGAAGTTCAAAGAGAAAGAGTTATTGGACCACTGAAACTCAAACTGCTAAGACAGAATCAGATGCAAAAATCACAATTGGACTTGCACCTGCTGAACTTGTTGTGGTTAATCCAAACATTAAAACTGCTGTTGGAAACGAAGTTGGTTATCGTTTGATTCCAGCAATTCCAGCTCATCCACTATTAACAGAGGATGATTATCCACAAATACGTGGTGCTTTTACAAACTATAATGTTTGGGTGACAGCATATAATAGAACTGAAAAATGGGCGGGTGGACTTTACGTTGATCATAGTCGCGGTGATGATACTTTGGCAGTTTGGACTAAACAGAATAGAGAGATTGTGAACAAGGACATAGTGATGTGGCATGTAGTTGGAATTCATCATGTTCCAGCACAGGAAGATTTCCCAATAATGCCACTATTGAGCACCTCATTTGAGCTGAGGCCAACCAATTTCTTTGAGAGGAATCCAGTTCTTAAAACACTTTCACCAAGAGATGTTGCATGGCCTGGTTGCTCCAATTAAAACTATCCAACATTCTGTATGGAACTGATTTGCATTGGCCTTGTGGTCATATGAATAAATGTTGTTTGTTTGTTTGTTGCATGTGGAAGAATAATGTATGGGTTTCAACTATTTTATTTGAATGAATGAATGAGAGGTTTATTTTAATAACGACTAGT
TARGET=2228,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06378 Ps_contig_mira-and-tgicl-ass_franssen_45118
SEQUENCE=ACTTCCTCTGCTGCTGGAGCTTCCACTGATTCAACTGTTTTGTTCTCAATCACATCCTCTGCAGTTGCTGGTGGTGGTGGTGCTGACTCATTTTCAGTAGTTGCTATTGGCTCCTCCACTACTGCTTCCGCCTCTTCCTTCACTTCTTCAACCGATTCCTCTAGTTTTCTTTAGGTGCTTCTAAGTTCTCTTCCTTTGCTTCCTCTGTTACCACTTTCCTTGTTCTCAACTTCAAGTGGATTCGTTTGGGGTTTCGGGANCATCTACAGCTTCTCCTGCTGCTAGTTTGTTTCTTGGTTTTGGGTTTTTCGGTTGTTTCTTGTTGGTTTGGTTACGTTCGGGTGG
TARGET=69,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3724
PRIMER_LEFT_PENALTY=0.262854
PRIMER_RIGHT_PENALTY=0.109510
PRIMER_LEFT_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_RIGHT_SEQUENCE=GGAATCGGTTGAAGAAGTGAAG
PRIMER_LEFT=40,22
PRIMER_RIGHT=158,22
PRIMER_LEFT_TM=60.263
PRIMER_RIGHT_TM=60.110
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=119
PRIMER_PAIR_PENALTY_1=0.4274
PRIMER_LEFT_1_PENALTY=0.262854
PRIMER_RIGHT_1_PENALTY=0.164560
PRIMER_LEFT_1_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_RIGHT_1_SEQUENCE=ACCAAACCAACAAGAAACAACC
PRIMER_LEFT_1=40,22
PRIMER_RIGHT_1=331,22
PRIMER_LEFT_1_TM=60.263
PRIMER_RIGHT_1_TM=60.165
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=8.2000
PRIMER_RIGHT_1_END_STABILITY=8.2000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=292
PRIMER_PAIR_PENALTY_2=0.4756
PRIMER_LEFT_2_PENALTY=0.262854
PRIMER_RIGHT_2_PENALTY=0.212766
PRIMER_LEFT_2_SEQUENCE=TGTTCTCAATCACATCCTCTGC
PRIMER_RIGHT_2_SEQUENCE=ACAAACTAGCAGCAGGAGAAGC
PRIMER_LEFT_2=40,22
PRIMER_RIGHT_2=289,22
PRIMER_LEFT_2_TM=60.263
PRIMER_RIGHT_2_TM=60.213
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=3.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=250
PRIMER_PAIR_PENALTY_3=0.6451
PRIMER_LEFT_3_PENALTY=0.642812
PRIMER_RIGHT_3_PENALTY=0.002310
PRIMER_LEFT_3_SEQUENCE=CTCAATCACATCCTCTGCAGTT
PRIMER_RIGHT_3_SEQUENCE=TTGAAGAAGTGAAGGAAGAGGC
PRIMER_LEFT_3=44,22
PRIMER_RIGHT_3=150,22
PRIMER_LEFT_3_TM=59.357
PRIMER_RIGHT_3_TM=59.998
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_3=107
PRIMER_PAIR_PENALTY_4=0.8074
PRIMER_LEFT_4_PENALTY=0.642812
PRIMER_RIGHT_4_PENALTY=0.164560
PRIMER_LEFT_4_SEQUENCE=CTCAATCACATCCTCTGCAGTT
PRIMER_RIGHT_4_SEQUENCE=ACCAAACCAACAAGAAACAACC
PRIMER_LEFT_4=44,22
PRIMER_RIGHT_4=331,22
PRIMER_LEFT_4_TM=59.357
PRIMER_RIGHT_4_TM=60.165
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=288
=
PRIMER_SEQUENCE_ID=SSR_06379 Ps_contig_mira-and-tgicl-ass_franssen_45144
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTTCTTTTTTACTGTGTACTCTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06380 Ps_contig_mira-and-tgicl-ass_franssen_45177
SEQUENCE=AAGAACAAAGTAGGGCTTCTCTTCGAGACTCAACAGAGTCTTCATCTCTTCAACTCACAACTAGGGTTTTCAGAAAACCCAAATCACGTTAACAATGTCGATGTCAAAGAGTTCCAAGATGCTTCAATACATCAACTACCGAATGAGGGTAACAATTCAAGACGGTCGTCAACTCGTCGGAAAGTTCATGGCCTTCGATCGACACATGAATCTCGTTCTTGGTGACTGTGAAGAGTTTCGCAAACTTCCGCCGGTGAAGGGGAAGAAAACGGCAGATGGAGACCGTGAAGACCGCCGTACACTTGGTCTTGTTCTACTTCGTGGTGAAGAGGTTGTGTCGATGACGGTTGAAGGTCCTCCTCCTCCTGAAGAGTCTAGGTCGAAGGGTGTTAATGCTGCGGCAATGGCTGGTCCTGGGATTGGTAGGGCTGCTGGGAGAGGTGTCCCTCCTGCTCCTGTTATTCAAGCTCAGCCAGGTTTGTCTGGTCCTATTCGTGGTGTTGGTGGACCTGCTCCCGGAATGATGCAGCCGCAGATCTCGCGGCCTCCTCAGATGTCGGCGCCTCCTGTTTCGTATCCCGGTGGTGCTCCGGTTATGCGTCCTCCTGGTCAGATGCCTTATCCTGGACAAGGTCCACCTCCGAGGGGGAAGGGTCCTCCTCCACCTATGCCGCCTGGACAATTTCCTCAGAGACCAGGTGGTCCTCCACCACAATTTTCCATGCCGCCTCCTCAGTATGGGCAGAGACCTATGGGCCCACCTCCTCCTGGACAGATGGTTAGAGGACCTCCTCCTCCACCTCGTCCTGGAATGCAAGCACCACCACGTCCTGGCATGCCTCCTCCTCCTGGAAGCGGTGTTCCGGTGTATGGTCCACCTCGGCCTGGCATGCCCCCTCCACCTAATGCTCCAAACCAACAACAGCAAAATTGATAGGATTCTTCTTGCTGGAGCTCTTAAGTTACGGACATTGGACTTCTTATTACAGATCAATATCCAACTCCGTGAGAGTAATTGTGACCTGTACAACCTTGAATCGGTCTCTTATTAGGGTTTTTATTGATGTTGTGATGAGAACGTTTATATTAGAACATGTATGTTTTGGAGGAAGATATTGTACCGAAACTCGAGCATTAAACATCCAAAACTGGCTGTGTAGATTGTTGGGTAATTTTGCTAAATCAGTGTGCTAATTTCTAAATTGTAGGGGATGGTTTGTCTCATTTAAGTGTTTTATGCTATATAACATAATTACCATTTTACCC
TARGET=355,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0749
PRIMER_LEFT_PENALTY=0.053733
PRIMER_RIGHT_PENALTY=0.021195
PRIMER_LEFT_SEQUENCE=CTCGTTCTTGGTGACTGTGAAG
PRIMER_RIGHT_SEQUENCE=AGCATTAACACCCTTCGACCTA
PRIMER_LEFT=211,22
PRIMER_RIGHT=396,22
PRIMER_LEFT_TM=59.946
PRIMER_RIGHT_TM=60.021
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=3.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.0000
PRIMER_RIGHT_END_STABILITY=6.9000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=186
PRIMER_PAIR_PENALTY_1=0.0749
PRIMER_LEFT_1_PENALTY=0.053733
PRIMER_RIGHT_1_PENALTY=0.021195
PRIMER_LEFT_1_SEQUENCE=CGTTCTTGGTGACTGTGAAGAG
PRIMER_RIGHT_1_SEQUENCE=AGCATTAACACCCTTCGACCTA
PRIMER_LEFT_1=213,22
PRIMER_RIGHT_1=396,22
PRIMER_LEFT_1_TM=59.946
PRIMER_RIGHT_1_TM=60.021
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.9000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=184
PRIMER_PAIR_PENALTY_2=0.1685
PRIMER_LEFT_2_PENALTY=0.147295
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=TCGATGTCAAAGAGTTCCAAGA
PRIMER_RIGHT_2_SEQUENCE=AGCATTAACACCCTTCGACCTA
PRIMER_LEFT_2=97,22
PRIMER_RIGHT_2=396,22
PRIMER_LEFT_2_TM=59.853
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=7.0000
PRIMER_RIGHT_2_END_STABILITY=6.9000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=300
PRIMER_PAIR_PENALTY_3=0.1897
PRIMER_LEFT_3_PENALTY=0.053733
PRIMER_RIGHT_3_PENALTY=0.136017
PRIMER_LEFT_3_SEQUENCE=CTCGTTCTTGGTGACTGTGAAG
PRIMER_RIGHT_3_SEQUENCE=GAATAGGACCAGACAAACCTGG
PRIMER_LEFT_3=211,22
PRIMER_RIGHT_3=493,22
PRIMER_LEFT_3_TM=59.946
PRIMER_RIGHT_3_TM=59.864
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=7.0000
PRIMER_RIGHT_3_END_STABILITY=9.7000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=283
PRIMER_PAIR_PENALTY_4=0.1897
PRIMER_LEFT_4_PENALTY=0.053733
PRIMER_RIGHT_4_PENALTY=0.136017
PRIMER_LEFT_4_SEQUENCE=CGTTCTTGGTGACTGTGAAGAG
PRIMER_RIGHT_4_SEQUENCE=GAATAGGACCAGACAAACCTGG
PRIMER_LEFT_4=213,22
PRIMER_RIGHT_4=493,22
PRIMER_LEFT_4_TM=59.946
PRIMER_RIGHT_4_TM=59.864
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=281
=
PRIMER_SEQUENCE_ID=SSR_06381 Ps_contig_mira-and-tgicl-ass_franssen_45177
SEQUENCE=AAGAACAAAGTAGGGCTTCTCTTCGAGACTCAACAGAGTCTTCATCTCTTCAACTCACAACTAGGGTTTTCAGAAAACCCAAATCACGTTAACAATGTCGATGTCAAAGAGTTCCAAGATGCTTCAATACATCAACTACCGAATGAGGGTAACAATTCAAGACGGTCGTCAACTCGTCGGAAAGTTCATGGCCTTCGATCGACACATGAATCTCGTTCTTGGTGACTGTGAAGAGTTTCGCAAACTTCCGCCGGTGAAGGGGAAGAAAACGGCAGATGGAGACCGTGAAGACCGCCGTACACTTGGTCTTGTTCTACTTCGTGGTGAAGAGGTTGTGTCGATGACGGTTGAAGGTCCTCCTCCTCCTGAAGAGTCTAGGTCGAAGGGTGTTAATGCTGCGGCAATGGCTGGTCCTGGGATTGGTAGGGCTGCTGGGAGAGGTGTCCCTCCTGCTCCTGTTATTCAAGCTCAGCCAGGTTTGTCTGGTCCTATTCGTGGTGTTGGTGGACCTGCTCCCGGAATGATGCAGCCGCAGATCTCGCGGCCTCCTCAGATGTCGGCGCCTCCTGTTTCGTATCCCGGTGGTGCTCCGGTTATGCGTCCTCCTGGTCAGATGCCTTATCCTGGACAAGGTCCACCTCCGAGGGGGAAGGGTCCTCCTCCACCTATGCCGCCTGGACAATTTCCTCAGAGACCAGGTGGTCCTCCACCACAATTTTCCATGCCGCCTCCTCAGTATGGGCAGAGACCTATGGGCCCACCTCCTCCTGGACAGATGGTTAGAGGACCTCCTCCTCCACCTCGTCCTGGAATGCAAGCACCACCACGTCCTGGCATGCCTCCTCCTCCTGGAAGCGGTGTTCCGGTGTATGGTCCACCTCGGCCTGGCATGCCCCCTCCACCTAATGCTCCAAACCAACAACAGCAAAATTGATAGGATTCTTCTTGCTGGAGCTCTTAAGTTACGGACATTGGACTTCTTATTACAGATCAATATCCAACTCCGTGAGAGTAATTGTGACCTGTACAACCTTGAATCGGTCTCTTATTAGGGTTTTTATTGATGTTGTGATGAGAACGTTTATATTAGAACATGTATGTTTTGGAGGAAGATATTGTACCGAAACTCGAGCATTAAACATCCAAAACTGGCTGTGTAGATTGTTGGGTAATTTTGCTAAATCAGTGTGCTAATTTCTAAATTGTAGGGGATGGTTTGTCTCATTTAAGTGTTTTATGCTATATAACATAATTACCATTTTACCC
TARGET=839,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0359
PRIMER_LEFT_PENALTY=0.005574
PRIMER_RIGHT_PENALTY=0.030276
PRIMER_LEFT_SEQUENCE=CCTGGACAGATGGTTAGAGGAC
PRIMER_RIGHT_SEQUENCE=CGATTCAAGGTTGTACAGGTCA
PRIMER_LEFT=766,22
PRIMER_RIGHT=1039,22
PRIMER_LEFT_TM=59.994
PRIMER_RIGHT_TM=60.030
PRIMER_LEFT_GC_PERCENT=54.545
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.6000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=274
PRIMER_PAIR_PENALTY_1=0.0359
PRIMER_LEFT_1_PENALTY=0.005574
PRIMER_RIGHT_1_PENALTY=0.030276
PRIMER_LEFT_1_SEQUENCE=CTGGACAGATGGTTAGAGGACC
PRIMER_RIGHT_1_SEQUENCE=CGATTCAAGGTTGTACAGGTCA
PRIMER_LEFT_1=767,22
PRIMER_RIGHT_1=1039,22
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=60.030
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.1000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=273
PRIMER_PAIR_PENALTY_2=0.0379
PRIMER_LEFT_2_PENALTY=0.005574
PRIMER_RIGHT_2_PENALTY=0.032347
PRIMER_LEFT_2_SEQUENCE=CCTGGACAGATGGTTAGAGGAC
PRIMER_RIGHT_2_SEQUENCE=TGTTGTTGGTTTGGAGCATTAG
PRIMER_LEFT_2=766,22
PRIMER_RIGHT_2=923,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.032
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.6000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=158
PRIMER_PAIR_PENALTY_3=0.0379
PRIMER_LEFT_3_PENALTY=0.005574
PRIMER_RIGHT_3_PENALTY=0.032347
PRIMER_LEFT_3_SEQUENCE=CCTGGACAGATGGTTAGAGGAC
PRIMER_RIGHT_3_SEQUENCE=CTGTTGTTGGTTTGGAGCATTA
PRIMER_LEFT_3=766,22
PRIMER_RIGHT_3=924,22
PRIMER_LEFT_3_TM=59.994
PRIMER_RIGHT_3_TM=60.032
PRIMER_LEFT_3_GC_PERCENT=54.545
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=7.6000
PRIMER_RIGHT_3_END_STABILITY=6.2000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=159
PRIMER_PAIR_PENALTY_4=0.0379
PRIMER_LEFT_4_PENALTY=0.005574
PRIMER_RIGHT_4_PENALTY=0.032347
PRIMER_LEFT_4_SEQUENCE=CTGGACAGATGGTTAGAGGACC
PRIMER_RIGHT_4_SEQUENCE=TGTTGTTGGTTTGGAGCATTAG
PRIMER_LEFT_4=767,22
PRIMER_RIGHT_4=923,22
PRIMER_LEFT_4_TM=59.994
PRIMER_RIGHT_4_TM=60.032
PRIMER_LEFT_4_GC_PERCENT=54.545
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.1000
PRIMER_RIGHT_4_END_STABILITY=5.9000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=157
=
PRIMER_SEQUENCE_ID=SSR_06382 Ps_contig_mira-and-tgicl-ass_franssen_45242
SEQUENCE=TTGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGGCTAATGAAAGAAAGAAAAGTATAGGTTGGTTGTAGATTACAGTATCTCTTATGTGTTGGTGACGTTTCTTTTTTTTTACGTTTGTGTACCTAACCTTT
TARGET=159,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=2.6832
PRIMER_LEFT_PENALTY=0.371199
PRIMER_RIGHT_PENALTY=2.311964
PRIMER_LEFT_SEQUENCE=ATACGTTCTTCAATGGCGAAAC
PRIMER_RIGHT_SEQUENCE=GAAACGTCACCAACACATAAGAG
PRIMER_LEFT=86,22
PRIMER_RIGHT=220,23
PRIMER_LEFT_TM=60.371
PRIMER_RIGHT_TM=58.688
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=43.478
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=135
PRIMER_PAIR_PENALTY_1=2.7046
PRIMER_LEFT_1_PENALTY=0.392624
PRIMER_RIGHT_1_PENALTY=2.311964
PRIMER_LEFT_1_SEQUENCE=AGTTGAAAGGATTGGTGTTTGC
PRIMER_RIGHT_1_SEQUENCE=GAAACGTCACCAACACATAAGAG
PRIMER_LEFT_1=8,22
PRIMER_RIGHT_1=220,23
PRIMER_LEFT_1_TM=60.393
PRIMER_RIGHT_1_TM=58.688
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=43.478
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=8.8000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=213
PRIMER_PAIR_PENALTY_2=2.7083
PRIMER_LEFT_2_PENALTY=0.371199
PRIMER_RIGHT_2_PENALTY=2.337082
PRIMER_LEFT_2_SEQUENCE=ATACGTTCTTCAATGGCGAAAC
PRIMER_RIGHT_2_SEQUENCE=GAAACGTCACCAACACATAAGA
PRIMER_LEFT_2=86,22
PRIMER_RIGHT_2=220,22
PRIMER_LEFT_2_TM=60.371
PRIMER_RIGHT_2_TM=57.663
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=135
PRIMER_PAIR_PENALTY_3=2.7297
PRIMER_LEFT_3_PENALTY=0.392624
PRIMER_RIGHT_3_PENALTY=2.337082
PRIMER_LEFT_3_SEQUENCE=AGTTGAAAGGATTGGTGTTTGC
PRIMER_RIGHT_3_SEQUENCE=GAAACGTCACCAACACATAAGA
PRIMER_LEFT_3=8,22
PRIMER_RIGHT_3=220,22
PRIMER_LEFT_3_TM=60.393
PRIMER_RIGHT_3_TM=57.663
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=8.8000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=213
PRIMER_PAIR_PENALTY_4=2.7470
PRIMER_LEFT_4_PENALTY=0.371199
PRIMER_RIGHT_4_PENALTY=2.375846
PRIMER_LEFT_4_SEQUENCE=ATACGTTCTTCAATGGCGAAAC
PRIMER_RIGHT_4_SEQUENCE=AGAAACGTCACCAACACATAAGAG
PRIMER_LEFT_4=86,22
PRIMER_RIGHT_4=221,24
PRIMER_LEFT_4_TM=60.371
PRIMER_RIGHT_4_TM=59.624
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=41.667
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=136
=
PRIMER_SEQUENCE_ID=SSR_06383 Ps_contig_mira-and-tgicl-ass_franssen_45247
SEQUENCE=ACCAAATGGAGGATCCATTGCAAAGCTGAGTGTGAAATACTTCACAAAAGGTGATGCTGCTCCTAGTGAAGAGCAACTCAAGACTGACAAAGCTAAGGGGGATGGTCTTTTCAAGGCTCTTGAGGGTTACTGTTTGGCTCATCCTGATTACAACTAAACTATATAATCATCAACAAGTGTGTTGTTATGTATACTATATAATCATCCTGTGTGCTTAATTTGGCTGCCAATGTTAATTTTCTTGTTTTTTCGTTTTTTTTCCTTTTTCTTTTTGTTGGTGGAAACTTAAAAGTGTGAGTATTGTAAGTCATGTACTACCTTCCTTCCTTTAC
TARGET=318,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06384 Ps_contig_mira-and-tgicl-ass_franssen_45256
SEQUENCE=TGTTAAGGTTTGTTAGTTAGTTAACTTGTAAGTAATTGGTAGGTTAGCGATGTTCTCAATGTTTGGATTCTTCGTTCAAGCTATTGTAACAGGAAAAGGACCAATAGAAAATCTTGCTGACCACCTGGCTGATCCAGTGAACAACAATGCATGGGCATATGCCACAAACTTTGCCCCTGGAAAGTGAGAGAAATAGACAATGATAAGTGACTAACTTGATATCGCCACAATGTGTAGA
TARGET=12,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06385 Ps_contig_mira-and-tgicl-ass_franssen_45299
SEQUENCE=TGTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAAGTATAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTCTTTTTTTTTACTGTGTACACTT
TARGET=158,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06386 Ps_contig_mira-and-tgicl-ass_franssen_45317
SEQUENCE=CGGTTCGGTTCGGTTCGTTCCTGGTAAACCGTTACGAAGTTGATGNTAACCACTAGATAAATAGATGCACATTCAAAGTAATAAGTTTCATGAATGAATAATGTTTGGTGTTAAATAAAAGAAATACCAATCTTGTTGTGGTTATGTATCATCTATGTATATGTGAAGACGTGTTTGATTTCGAGTAAAAGTGATGAATGTGTTATCATTTGTTATATATCAAATATCATCCTTGTTGTTACTTTGCCTCCTTTTAAATTTATATCTCCATACTGCTGAGTTATGGTTAGTCCATATGAATTACAGGTGATCTGGTTAACCCCCATAATTATAAAATTTAAAAAGTTATTTTAATATAC
TARGET=1,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06387 Ps_contig_mira-and-tgicl-ass_franssen_45320
SEQUENCE=TAAAATACATTTTTTTAGTAAAAAAGTAGTAATAGTAGAACGTAATATTTTATTTAATTTTGGTGATCCCTTCAAACGAGTAGGTTGCAAAATACATTACACACATGCCTTAGAACTGTCTAAGTTCAAAAACCAAACAAACTAACAATGAGAATAGCAAAACACATGAGATAAAAAGAGTGGCTTTTCTTGAAACAAACTACGTTGAAACTTTGCTTCCTTGTATTATTGCCAATGCATCTTCCTGCTTATTTCCAATATATACCTAAAATCCAAGGGATCTACTATAAACCAGTATCTAGTAGTGGTATGGTGGAGGAGGTGATGCGTAGATGTAATGTGGTGGAGGAGGGGAATAGACCGGTGGAGGTGATGAGTAAACAGGTGGAGGAGGAGACTTATATTTGTAAATGGGTGGTGGCGAGTAAACTGGTGGAGGAGGAGATTTATATTTGTAAACTGGTGGTGGTGAATAAATCGGTGGAGGAGAAGATTTATATTTGTAAATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACTGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGCGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACTGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACCGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTGACTTGTATTTGTAAACTGGTGGTGGAGGAGAAGGATATTTGTATGGCTTCTCTGACGGTGGTGGTGGTGAATTGTAATGATAAGGTGGAGGAGGTGAGTGTACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATGAATAGATGTAGTTGTTTGCTTGAGATGGCAAAGTGAGAGAAAGTAATGTGAATGCAATAGTGAGAGTAATAGAGGCCATTTGAGACCCCATGTTTTCTCAAAGTAGATTTTGTTTTTGCTCCCCGAACTCTGCGTTGA
TARGET=860,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4676
PRIMER_LEFT_PENALTY=0.296849
PRIMER_RIGHT_PENALTY=0.170703
PRIMER_LEFT_SEQUENCE=TTTGTAAACTGGTGGTGGTGAA
PRIMER_RIGHT_SEQUENCE=GCCTCCTCCATACAAATATCCA
PRIMER_LEFT=637,22
PRIMER_RIGHT=925,22
PRIMER_LEFT_TM=60.297
PRIMER_RIGHT_TM=60.171
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.4884
PRIMER_LEFT_1_PENALTY=0.005574
PRIMER_RIGHT_1_PENALTY=0.482784
PRIMER_LEFT_1_SEQUENCE=GTGAATAGACAGGTGGAGGAGG
PRIMER_RIGHT_1_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_1=699,22
PRIMER_RIGHT_1=976,22
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=60.483
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.4884
PRIMER_LEFT_2_PENALTY=0.005574
PRIMER_RIGHT_2_PENALTY=0.482784
PRIMER_LEFT_2_SEQUENCE=GGTGAATAGACAGGTGGAGGAG
PRIMER_RIGHT_2_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_2=698,22
PRIMER_RIGHT_2=976,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.6561
PRIMER_LEFT_3_PENALTY=0.173354
PRIMER_RIGHT_3_PENALTY=0.482784
PRIMER_LEFT_3_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_3_SEQUENCE=CCTCCACCGGTATACAATTCAC
PRIMER_LEFT_3=827,22
PRIMER_RIGHT_3=978,22
PRIMER_LEFT_3_TM=59.827
PRIMER_RIGHT_3_TM=60.483
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=0.6561
PRIMER_LEFT_4_PENALTY=0.173354
PRIMER_RIGHT_4_PENALTY=0.482784
PRIMER_LEFT_4_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_4_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_4=827,22
PRIMER_RIGHT_4=976,22
PRIMER_LEFT_4_TM=59.827
PRIMER_RIGHT_4_TM=60.483
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_06388 Ps_contig_mira-and-tgicl-ass_franssen_45320
SEQUENCE=TAAAATACATTTTTTTAGTAAAAAAGTAGTAATAGTAGAACGTAATATTTTATTTAATTTTGGTGATCCCTTCAAACGAGTAGGTTGCAAAATACATTACACACATGCCTTAGAACTGTCTAAGTTCAAAAACCAAACAAACTAACAATGAGAATAGCAAAACACATGAGATAAAAAGAGTGGCTTTTCTTGAAACAAACTACGTTGAAACTTTGCTTCCTTGTATTATTGCCAATGCATCTTCCTGCTTATTTCCAATATATACCTAAAATCCAAGGGATCTACTATAAACCAGTATCTAGTAGTGGTATGGTGGAGGAGGTGATGCGTAGATGTAATGTGGTGGAGGAGGGGAATAGACCGGTGGAGGTGATGAGTAAACAGGTGGAGGAGGAGACTTATATTTGTAAATGGGTGGTGGCGAGTAAACTGGTGGAGGAGGAGATTTATATTTGTAAACTGGTGGTGGTGAATAAATCGGTGGAGGAGAAGATTTATATTTGTAAATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACTGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGCGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACTGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACCGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTGACTTGTATTTGTAAACTGGTGGTGGAGGAGAAGGATATTTGTATGGCTTCTCTGACGGTGGTGGTGGTGAATTGTAATGATAAGGTGGAGGAGGTGAGTGTACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATGAATAGATGTAGTTGTTTGCTTGAGATGGCAAAGTGAGAGAAAGTAATGTGAATGCAATAGTGAGAGTAATAGAGGCCATTTGAGACCCCATGTTTTCTCAAAGTAGATTTTGTTTTTGCTCCCCGAACTCTGCGTTGA
TARGET=891,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.4676
PRIMER_LEFT_PENALTY=0.296849
PRIMER_RIGHT_PENALTY=0.170703
PRIMER_LEFT_SEQUENCE=TTTGTAAACTGGTGGTGGTGAA
PRIMER_RIGHT_SEQUENCE=GCCTCCTCCATACAAATATCCA
PRIMER_LEFT=637,22
PRIMER_RIGHT=925,22
PRIMER_LEFT_TM=60.297
PRIMER_RIGHT_TM=60.171
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.1000
PRIMER_PAIR_COMPL_ANY=6.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=289
PRIMER_PAIR_PENALTY_1=0.4884
PRIMER_LEFT_1_PENALTY=0.005574
PRIMER_RIGHT_1_PENALTY=0.482784
PRIMER_LEFT_1_SEQUENCE=GTGAATAGACAGGTGGAGGAGG
PRIMER_RIGHT_1_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_1=699,22
PRIMER_RIGHT_1=976,22
PRIMER_LEFT_1_TM=59.994
PRIMER_RIGHT_1_TM=60.483
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.4000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=8.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=278
PRIMER_PAIR_PENALTY_2=0.4884
PRIMER_LEFT_2_PENALTY=0.005574
PRIMER_RIGHT_2_PENALTY=0.482784
PRIMER_LEFT_2_SEQUENCE=GGTGAATAGACAGGTGGAGGAG
PRIMER_RIGHT_2_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_2=698,22
PRIMER_RIGHT_2=976,22
PRIMER_LEFT_2_TM=59.994
PRIMER_RIGHT_2_TM=60.483
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=7.9000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=279
PRIMER_PAIR_PENALTY_3=0.6561
PRIMER_LEFT_3_PENALTY=0.173354
PRIMER_RIGHT_3_PENALTY=0.482784
PRIMER_LEFT_3_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_3_SEQUENCE=CCTCCACCGGTATACAATTCAC
PRIMER_LEFT_3=827,22
PRIMER_RIGHT_3=978,22
PRIMER_LEFT_3_TM=59.827
PRIMER_RIGHT_3_TM=60.483
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=152
PRIMER_PAIR_PENALTY_4=0.6561
PRIMER_LEFT_4_PENALTY=0.173354
PRIMER_RIGHT_4_PENALTY=0.482784
PRIMER_LEFT_4_SEQUENCE=ATTGGTGTTGGAGGAGGAGATA
PRIMER_RIGHT_4_SEQUENCE=TCCACCGGTATACAATTCACCT
PRIMER_LEFT_4=827,22
PRIMER_RIGHT_4=976,22
PRIMER_LEFT_4_TM=59.827
PRIMER_RIGHT_4_TM=60.483
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=7.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=150
=
PRIMER_SEQUENCE_ID=SSR_06389 Ps_contig_mira-and-tgicl-ass_franssen_45320
SEQUENCE=TAAAATACATTTTTTTAGTAAAAAAGTAGTAATAGTAGAACGTAATATTTTATTTAATTTTGGTGATCCCTTCAAACGAGTAGGTTGCAAAATACATTACACACATGCCTTAGAACTGTCTAAGTTCAAAAACCAAACAAACTAACAATGAGAATAGCAAAACACATGAGATAAAAAGAGTGGCTTTTCTTGAAACAAACTACGTTGAAACTTTGCTTCCTTGTATTATTGCCAATGCATCTTCCTGCTTATTTCCAATATATACCTAAAATCCAAGGGATCTACTATAAACCAGTATCTAGTAGTGGTATGGTGGAGGAGGTGATGCGTAGATGTAATGTGGTGGAGGAGGGGAATAGACCGGTGGAGGTGATGAGTAAACAGGTGGAGGAGGAGACTTATATTTGTAAATGGGTGGTGGCGAGTAAACTGGTGGAGGAGGAGATTTATATTTGTAAACTGGTGGTGGTGAATAAATCGGTGGAGGAGAAGATTTATATTTGTAAATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACTGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGCGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACTGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACCGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTGACTTGTATTTGTAAACTGGTGGTGGAGGAGAAGGATATTTGTATGGCTTCTCTGACGGTGGTGGTGGTGAATTGTAATGATAAGGTGGAGGAGGTGAGTGTACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATGAATAGATGTAGTTGTTTGCTTGAGATGGCAAAGTGAGAGAAAGTAATGTGAATGCAATAGTGAGAGTAATAGAGGCCATTTGAGACCCCATGTTTTCTCAAAGTAGATTTTGTTTTTGCTCCCCGAACTCTGCGTTGA
TARGET=1041,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0814
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=0.006945
PRIMER_LEFT_SEQUENCE=ATATGGTGGAGGAGGTGAATTG
PRIMER_RIGHT_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_LEFT=943,22
PRIMER_RIGHT=1193,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.1156
PRIMER_LEFT_1_PENALTY=0.108684
PRIMER_RIGHT_1_PENALTY=0.006945
PRIMER_LEFT_1_SEQUENCE=TGGAGGTGGTGACTTGTATTTG
PRIMER_RIGHT_1_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_LEFT_1=973,22
PRIMER_RIGHT_1=1193,22
PRIMER_LEFT_1_TM=59.891
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.1509
PRIMER_LEFT_2_PENALTY=0.143968
PRIMER_RIGHT_2_PENALTY=0.006945
PRIMER_LEFT_2_SEQUENCE=ATTTGTAAACTGGTGGTGGAGG
PRIMER_RIGHT_2_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_LEFT_2=990,22
PRIMER_RIGHT_2=1193,22
PRIMER_LEFT_2_TM=60.144
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=204
PRIMER_PAIR_PENALTY_3=0.1601
PRIMER_LEFT_3_PENALTY=0.153161
PRIMER_RIGHT_3_PENALTY=0.006945
PRIMER_LEFT_3_SEQUENCE=GGTGGAGGTGGTGACTTGTATT
PRIMER_RIGHT_3_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_LEFT_3=971,22
PRIMER_RIGHT_3=1193,22
PRIMER_LEFT_3_TM=60.153
PRIMER_RIGHT_3_TM=59.993
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.1776
PRIMER_LEFT_4_PENALTY=0.170703
PRIMER_RIGHT_4_PENALTY=0.006945
PRIMER_LEFT_4_SEQUENCE=TGGATATTTGTATGGAGGAGGC
PRIMER_RIGHT_4_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_LEFT_4=904,22
PRIMER_RIGHT_4=1193,22
PRIMER_LEFT_4_TM=60.171
PRIMER_RIGHT_4_TM=59.993
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_06390 Ps_contig_mira-and-tgicl-ass_franssen_45320
SEQUENCE=TAAAATACATTTTTTTAGTAAAAAAGTAGTAATAGTAGAACGTAATATTTTATTTAATTTTGGTGATCCCTTCAAACGAGTAGGTTGCAAAATACATTACACACATGCCTTAGAACTGTCTAAGTTCAAAAACCAAACAAACTAACAATGAGAATAGCAAAACACATGAGATAAAAAGAGTGGCTTTTCTTGAAACAAACTACGTTGAAACTTTGCTTCCTTGTATTATTGCCAATGCATCTTCCTGCTTATTTCCAATATATACCTAAAATCCAAGGGATCTACTATAAACCAGTATCTAGTAGTGGTATGGTGGAGGAGGTGATGCGTAGATGTAATGTGGTGGAGGAGGGGAATAGACCGGTGGAGGTGATGAGTAAACAGGTGGAGGAGGAGACTTATATTTGTAAATGGGTGGTGGCGAGTAAACTGGTGGAGGAGGAGATTTATATTTGTAAACTGGTGGTGGTGAATAAATCGGTGGAGGAGAAGATTTATATTTGTAAATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACTGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGCGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACTGGTGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGAATAGACAGGTGGAGGAGGAGACTTATATTTGTAAACCGGTGGTGAATAAACCGGTGGAGGAGGAGATTTATATTTGTAAATTGGTGGTGCATAGACACGTGGAGGAGGAGACTTATATTTATAAATTGGTGTTGGAGGAGGAGATATATATTTGTAAGGAGGAGGAGGTGAGTTGTACTTGTAAACAGGTGGTGGTGGTGATGGATATTTGTATGGAGGAGGCGGTGGAGATGGATACTTATATGGTGGAGGAGGTGAATTGTATACCGGTGGAGGTGGTGACTTGTATTTGTAAACTGGTGGTGGAGGAGAAGGATATTTGTATGGCTTCTCTGACGGTGGTGGTGGTGAATTGTAATGATAAGGTGGAGGAGGTGAGTGTACTGGTGGTGGTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATGAATAGATGTAGTTGTTTGCTTGAGATGGCAAAGTGAGAGAAAGTAATGTGAATGCAATAGTGAGAGTAATAGAGGCCATTTGAGACCCCATGTTTTCTCAAAGTAGATTTTGTTTTTGCTCCCCGAACTCTGCGTTGA
TARGET=1088,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.0814
PRIMER_LEFT_PENALTY=0.074466
PRIMER_RIGHT_PENALTY=0.006945
PRIMER_LEFT_SEQUENCE=ATATGGTGGAGGAGGTGAATTG
PRIMER_RIGHT_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_LEFT=943,22
PRIMER_RIGHT=1193,22
PRIMER_LEFT_TM=60.074
PRIMER_RIGHT_TM=59.993
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=7.2000
PRIMER_RIGHT_END_STABILITY=6.7000
PRIMER_PAIR_COMPL_ANY=4.00
PRIMER_PAIR_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=0.1156
PRIMER_LEFT_1_PENALTY=0.108684
PRIMER_RIGHT_1_PENALTY=0.006945
PRIMER_LEFT_1_SEQUENCE=TGGAGGTGGTGACTTGTATTTG
PRIMER_RIGHT_1_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_LEFT_1=973,22
PRIMER_RIGHT_1=1193,22
PRIMER_LEFT_1_TM=59.891
PRIMER_RIGHT_1_TM=59.993
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.2000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=221
PRIMER_PAIR_PENALTY_2=0.1509
PRIMER_LEFT_2_PENALTY=0.143968
PRIMER_RIGHT_2_PENALTY=0.006945
PRIMER_LEFT_2_SEQUENCE=ATTTGTAAACTGGTGGTGGAGG
PRIMER_RIGHT_2_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_LEFT_2=990,22
PRIMER_RIGHT_2=1193,22
PRIMER_LEFT_2_TM=60.144
PRIMER_RIGHT_2_TM=59.993
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=6.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_2=204
PRIMER_PAIR_PENALTY_3=0.1601
PRIMER_LEFT_3_PENALTY=0.153161
PRIMER_RIGHT_3_PENALTY=0.006945
PRIMER_LEFT_3_SEQUENCE=GGTGGAGGTGGTGACTTGTATT
PRIMER_RIGHT_3_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_LEFT_3=971,22
PRIMER_RIGHT_3=1193,22
PRIMER_LEFT_3_TM=60.153
PRIMER_RIGHT_3_TM=59.993
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=3.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.6000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=223
PRIMER_PAIR_PENALTY_4=0.1776
PRIMER_LEFT_4_PENALTY=0.170703
PRIMER_RIGHT_4_PENALTY=0.006945
PRIMER_LEFT_4_SEQUENCE=TGGATATTTGTATGGAGGAGGC
PRIMER_RIGHT_4_SEQUENCE=TTTCTCTCACTTTGCCATCTCA
PRIMER_LEFT_4=904,22
PRIMER_RIGHT_4=1193,22
PRIMER_LEFT_4_TM=60.171
PRIMER_RIGHT_4_TM=59.993
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=40.909
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=290
=
PRIMER_SEQUENCE_ID=SSR_06391 Ps_contig_mira-and-tgicl-ass_franssen_45343
SEQUENCE=TAGGATGGAGACTCACCGGAGAATGGGCCTAAGTACTTAACACGGTCTGGTCCGTACCATGGGCTTCCAGAGGAAGCTACTTTCTTGGTGGTAGCAGACTTCCTCATGGTGAACCTTGCAGCTCCCAATTCTTGGCTTGATGGGGTCAGCTTGAGTTGCTTGCCAGCCAAGGTTGGAGAAGAGAGAGCCATGGATGATGATGATGATGCGGCCATCGTGTATTATATTGCAATGAAATTGAAAAG
TARGET=193,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.6935
PRIMER_LEFT_PENALTY=0.301926
PRIMER_RIGHT_PENALTY=1.391547
PRIMER_LEFT_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_RIGHT_SEQUENCE=GCAATATAATACACGATGGCCG
PRIMER_LEFT=82,22
PRIMER_RIGHT=229,22
PRIMER_LEFT_TM=60.302
PRIMER_RIGHT_TM=61.392
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=2.00
PRIMER_LEFT_END_STABILITY=8.2000
PRIMER_RIGHT_END_STABILITY=12.9000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=2.00
PRIMER_PRODUCT_SIZE=148
PRIMER_PAIR_PENALTY_1=1.6935
PRIMER_LEFT_1_PENALTY=0.301926
PRIMER_RIGHT_1_PENALTY=1.391547
PRIMER_LEFT_1_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_RIGHT_1_SEQUENCE=GCAATATAATACACGATGGCCG
PRIMER_LEFT_1=83,22
PRIMER_RIGHT_1=229,22
PRIMER_LEFT_1_TM=60.302
PRIMER_RIGHT_1_TM=61.392
PRIMER_LEFT_1_GC_PERCENT=54.545
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=7.9000
PRIMER_RIGHT_1_END_STABILITY=12.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=147
PRIMER_PAIR_PENALTY_2=1.9806
PRIMER_LEFT_2_PENALTY=0.589035
PRIMER_RIGHT_2_PENALTY=1.391547
PRIMER_LEFT_2_SEQUENCE=GAGGAAGCTACTTTCTTGGTGG
PRIMER_RIGHT_2_SEQUENCE=GCAATATAATACACGATGGCCG
PRIMER_LEFT_2=69,22
PRIMER_RIGHT_2=229,22
PRIMER_LEFT_2_TM=59.411
PRIMER_RIGHT_2_TM=61.392
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=9.4000
PRIMER_RIGHT_2_END_STABILITY=12.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=161
PRIMER_PAIR_PENALTY_3=2.0523
PRIMER_LEFT_3_PENALTY=0.660713
PRIMER_RIGHT_3_PENALTY=1.391547
PRIMER_LEFT_3_SEQUENCE=TTCTTGGTGGTAGCAGACTTCC
PRIMER_RIGHT_3_SEQUENCE=GCAATATAATACACGATGGCCG
PRIMER_LEFT_3=81,22
PRIMER_RIGHT_3=229,22
PRIMER_LEFT_3_TM=60.661
PRIMER_RIGHT_3_TM=61.392
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=8.2000
PRIMER_RIGHT_3_END_STABILITY=12.9000
PRIMER_PAIR_3_COMPL_ANY=3.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=149
PRIMER_PAIR_PENALTY_4=2.4303
PRIMER_LEFT_4_PENALTY=1.038744
PRIMER_RIGHT_4_PENALTY=1.391547
PRIMER_LEFT_4_SEQUENCE=GCTACTTTCTTGGTGGTAGCAGA
PRIMER_RIGHT_4_SEQUENCE=GCAATATAATACACGATGGCCG
PRIMER_LEFT_4=75,23
PRIMER_RIGHT_4=229,22
PRIMER_LEFT_4_TM=59.961
PRIMER_RIGHT_4_TM=61.392
PRIMER_LEFT_4_GC_PERCENT=47.826
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=12.9000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=155
=
PRIMER_SEQUENCE_ID=SSR_06392 Ps_contig_mira-and-tgicl-ass_franssen_45455
SEQUENCE=GTCGGGGGACTATTGATCTCACAACATTCATTCATTCACCATGGCAATGGCAACACAAGCCTCTCTCTTCACACCACCCAACTCTCAACCCCCAAAACATGGAAACAACCCTCCACTCTATCTTTCATCTCTCTCAAACCAACCAAGTTCACACCAAGAACAACAAAAATTGCTGCAACAGAAGACAAAACCGATGCAGCAACCGATAGTAGACAAACAAAAAGAGCAGCCRACCTAAGGTTGGTACTTA
TARGET=25,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06393 Ps_contig_mira-and-tgicl-ass_franssen_45470
SEQUENCE=ATGGGATTTGTTGAAGGCTGCAGAGTTTGGTAGGTAGGTACCCCTTCGGTCGTAAGGTACTTAGTACCCACTTTACCCTGTGGTGCTTTTGACCCTCTTTGGCCTTGTCTGATGACCCTGATTCATTTGCAGAGTTGAAGGTCAAGGAACTCAAGAATGGTTCGGCTGGCTATGTTCTCCATGTTTGGTTTCTTTGTTCAAGCCATTGTTACTGGCAAGGGCCCTATTCAGAACCTTTTATGACCATGTTGCTGACCCTGTCGCGAACAACG
TARGET=29,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=1.0113
PRIMER_LEFT_PENALTY=1.007669
PRIMER_RIGHT_PENALTY=0.003589
PRIMER_LEFT_SEQUENCE=GATTTGTTGAAGGCTGCAGAG
PRIMER_RIGHT_SEQUENCE=TCAGCAACATGGTCATAAAAGG
PRIMER_LEFT=4,21
PRIMER_RIGHT=254,22
PRIMER_LEFT_TM=60.008
PRIMER_RIGHT_TM=59.996
PRIMER_LEFT_GC_PERCENT=47.619
PRIMER_RIGHT_GC_PERCENT=40.909
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_COMPL_ANY=5.00
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=251
PRIMER_PAIR_PENALTY_1=1.0120
PRIMER_LEFT_1_PENALTY=1.007669
PRIMER_RIGHT_1_PENALTY=0.004359
PRIMER_LEFT_1_SEQUENCE=GATTTGTTGAAGGCTGCAGAG
PRIMER_RIGHT_1_SEQUENCE=AACTCTGCAAATGAATCAGGGT
PRIMER_LEFT_1=4,21
PRIMER_RIGHT_1=135,22
PRIMER_LEFT_1_TM=60.008
PRIMER_RIGHT_1_TM=60.004
PRIMER_LEFT_1_GC_PERCENT=47.619
PRIMER_RIGHT_1_GC_PERCENT=40.909
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=5.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=9.1000
PRIMER_PAIR_1_COMPL_ANY=7.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=132
PRIMER_PAIR_PENALTY_2=1.0289
PRIMER_LEFT_2_PENALTY=1.007669
PRIMER_RIGHT_2_PENALTY=0.021195
PRIMER_LEFT_2_SEQUENCE=GATTTGTTGAAGGCTGCAGAG
PRIMER_RIGHT_2_SEQUENCE=GGCTTGAACAAAGAAACCAAAC
PRIMER_LEFT_2=4,21
PRIMER_RIGHT_2=203,22
PRIMER_LEFT_2_TM=60.008
PRIMER_RIGHT_2_TM=60.021
PRIMER_LEFT_2_GC_PERCENT=47.619
PRIMER_RIGHT_2_GC_PERCENT=40.909
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=8.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=200
PRIMER_PAIR_PENALTY_3=1.0751
PRIMER_LEFT_3_PENALTY=1.007669
PRIMER_RIGHT_3_PENALTY=0.067436
PRIMER_LEFT_3_SEQUENCE=GATTTGTTGAAGGCTGCAGAG
PRIMER_RIGHT_3_SEQUENCE=CAAATGAATCAGGGTCATCAGA
PRIMER_LEFT_3=4,21
PRIMER_RIGHT_3=128,22
PRIMER_LEFT_3_TM=60.008
PRIMER_RIGHT_3_TM=59.933
PRIMER_LEFT_3_GC_PERCENT=47.619
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=6.7000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=125
PRIMER_PAIR_PENALTY_4=1.1187
PRIMER_LEFT_4_PENALTY=1.007669
PRIMER_RIGHT_4_PENALTY=0.111077
PRIMER_LEFT_4_SEQUENCE=GATTTGTTGAAGGCTGCAGAG
PRIMER_RIGHT_4_SEQUENCE=TGAATCAGGGTCATCAGACAAG
PRIMER_LEFT_4=4,21
PRIMER_RIGHT_4=124,22
PRIMER_LEFT_4_TM=60.008
PRIMER_RIGHT_4_TM=60.111
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=45.455
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=121
=
PRIMER_SEQUENCE_ID=SSR_06394 Ps_contig_mira-and-tgicl-ass_franssen_45485
SEQUENCE=TTATTTCCTCCGAGGTTTCAGCTAGAGAATTAGCTGAGACTTCCACTAATGCCAAAGAGGAAGTTGCTGAAAAGTCAAATGAAGTAAATGATGCCAAATATTACGGTGGAGGTTATGGTCATGGCTATGGTCATGGACATGGAGGCTACGGTCATGGAGGCTACGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGARGTGCANTGGYRNTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAAGGTTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTTGCTTGAGATGGCAAGGTGAGAGAGAGTATTGTCAATGCAATAGTGAGAGTAATAGAGGTCATTTGAGACCCCATGTTTTCTCARAATNAGGTTTTTGTTAGTTRCCCCGAAC
TARGET=251,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06395 Ps_contig_mira-and-tgicl-ass_franssen_45485
SEQUENCE=TTATTTCCTCCGAGGTTTCAGCTAGAGAATTAGCTGAGACTTCCACTAATGCCAAAGAGGAAGTTGCTGAAAAGTCAAATGAAGTAAATGATGCCAAATATTACGGTGGAGGTTATGGTCATGGCTATGGTCATGGACATGGAGGCTACGGTCATGGAGGCTACGGTCATGGTGGTGGAGGTTACGGTCATGGTGGTGACTTATACTTGTAAACTGGTGGTGGAGGAGAAGAATATTTGTACGACTTCTTTGGTGGTGGTGGTGGTGAACTATAGTGATAAGGTGGAGGARGTGCANTGGYRNTGGTGAACTATAATGATAAGGTGGAGGAGGTGAGTGCACCGGTGGTGGTGGAGATTGGTAGTAGTAAGGTTTTGGTGGTGGTGGTGGAGATGAATAGAGGTAATTGTTTGCTTGAGATGGCAAGGTGAGAGAGAGTATTGTCAATGCAATAGTGAGAGTAATAGAGGTCATTTGAGACCCCATGTTTTCTCARAATNAGGTTTTTGTTAGTTRCCCCGAAC
TARGET=376,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06396 Ps_contig_mira-and-tgicl-ass_franssen_45525
SEQUENCE=AAGCAGTGGTATCAACGTTTCTTTTTTAGTTTTAGNTCGGATCACGAATTCAAATTCTAACCCTAAATTCCCAATCACAAAATTTCAATCGAAAATGTCAGGTCGTGGTAAGGGTGGTAAGGGACTAGGAAAGGGAGGTGCAAAGAGGCACAGGAAGGTGTTGCGAGACAACATCCAAGGTATCACCAAGCCAGCGATCCGTAGATTGGCGAGAAGAGGAGGTGTGAAGAGAATCAGCGGTTTGATCTATGAAGAAACAAGAGGTGTCTTGAAGATCTTTTTGGAGAATGTGATTCGTGATGCTGTTACATATACCGAACACGCTAGGAGGAAGACGGTTACAGCTATGGATGTTGTTTACGCTCTCAAGAGACAGGGAAGAACCTTGTACGGATTTGGAGGTTGAAGAATGTACAAGAAGAAGAAGTTCTCTTATGCTTTTTCTTATTGACTATGTGTAATTTGAATATGTGAATTTTCTTTAGAGGTTTATGATGGTCATTAGGATTATCATGTGCTTTTATAATGCTGAATGAGATTGATGTATATTCAGTTTACCCCTAAATTTAAAGATGAAATACAAAATGAAGGAGTTATTTTATAA
TARGET=416,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06397 Ps_contig_mira-and-tgicl-ass_franssen_45530
SEQUENCE=TGTTTTGGTAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACAGAGAAATACTCTGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTNTAATTGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTTCTTTTTTTACNGTGTACACTT
TARGET=161,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06398 Ps_contig_mira-and-tgicl-ass_franssen_45561
SEQUENCE=AGTGACAYCGTCGCCGGACCCTTTGTCCGGACGGAATAGGTCTCGGCAAGCTCCGAAAACAACCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGAATACAGAGGGTGGAAACGTGAGATCCTACGGTGGGGATTCGTTAGAGGGTGCAGTGAAAGTGACACGTAGTATCATGATAGTGAAACCAGCTGGGTACCAGAGTCCAAGTGGATCAGCTCCGGCTTCCCCCGCTGGTTCTACTCCGCCGACTAATCTCCGTTTTCCGGAGCTAGAGAGCCGTTTCGTTTTCGAAGAA
TARGET=64,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06399 Ps_contig_mira-and-tgicl-ass_franssen_45579
SEQUENCE=TGTTTGGAAGTTGAAAGGATTGGTGTTTGCCATCAAGCAGGATCGGATAGTTTACTCACTGCTTGTGCATTTAGAAAATTGAGGGATACGTTCTTCAATGGCGAAACNGAGAAATACTCTGGGTGTGTTGTATGGATTAGGTGTTGAGACAGACTAATTGAAAGAAAGAAAGTNTAATTGGGTTGTAGATTACAGTATCTCTTATGTGTTGTGAGTTTCTTTTTTTTTANGTGTACACTTTA
TARGET=160,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06400 Ps_contig_mira-and-tgicl-ass_franssen_45603
SEQUENCE=CGACANAGTACGCGGGGGTCGTGCGCTTTCCATATTCTCATGAGCCTTCTCGATCACTTGTGGGATGACACCGTCGCCGGACCTTTGCCGGAGAATGGTCTCGGCAAGCTCCGAAAACACCCTTCCTTCCTTTCCCGATCTATCTCCGACAAGGAATCAGAGGGTGGAAACGTGAGATCCTACGGTGGGGATTCGTTAGAGGGTGCAGTGAAAGTGACACGTAGTATCATGATAGTGAAACCAGCTGGGTACCAGAGTCCAAGTG
TARGET=121,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.3621
PRIMER_LEFT_PENALTY=0.275745
PRIMER_RIGHT_PENALTY=0.086339
PRIMER_LEFT_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_SEQUENCE=TCACTTTCACTGCACCCTCTAA
PRIMER_LEFT=39,22
PRIMER_RIGHT=216,22
PRIMER_LEFT_TM=60.276
PRIMER_RIGHT_TM=59.914
PRIMER_LEFT_GC_PERCENT=45.455
PRIMER_RIGHT_GC_PERCENT=45.455
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_COMPL_ANY=3.00
PRIMER_PAIR_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=178
PRIMER_PAIR_PENALTY_1=0.3840
PRIMER_LEFT_1_PENALTY=0.275745
PRIMER_RIGHT_1_PENALTY=0.108206
PRIMER_LEFT_1_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_1_SEQUENCE=ACCCAGCTGGTTTCACTATCAT
PRIMER_LEFT_1=39,22
PRIMER_RIGHT_1=249,22
PRIMER_LEFT_1_TM=60.276
PRIMER_RIGHT_1_TM=59.892
PRIMER_LEFT_1_GC_PERCENT=45.455
PRIMER_RIGHT_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=3.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=6.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_1=211
PRIMER_PAIR_PENALTY_2=0.6439
PRIMER_LEFT_2_PENALTY=0.275745
PRIMER_RIGHT_2_PENALTY=0.368190
PRIMER_LEFT_2_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_2_SEQUENCE=TACCCAGCTGGTTTCACTATCA
PRIMER_LEFT_2=39,22
PRIMER_RIGHT_2=250,22
PRIMER_LEFT_2_TM=60.276
PRIMER_RIGHT_2_TM=59.632
PRIMER_LEFT_2_GC_PERCENT=45.455
PRIMER_RIGHT_2_GC_PERCENT=45.455
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=8.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.7000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=212
PRIMER_PAIR_PENALTY_3=0.6994
PRIMER_LEFT_3_PENALTY=0.275745
PRIMER_RIGHT_3_PENALTY=0.423703
PRIMER_LEFT_3_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_3_SEQUENCE=ATTCCTTGTCGGAGATAGATCG
PRIMER_LEFT_3=39,22
PRIMER_RIGHT_3=156,22
PRIMER_LEFT_3_TM=60.276
PRIMER_RIGHT_3_TM=59.576
PRIMER_LEFT_3_GC_PERCENT=45.455
PRIMER_RIGHT_3_GC_PERCENT=45.455
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=6.7000
PRIMER_RIGHT_3_END_STABILITY=8.3000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=118
PRIMER_PAIR_PENALTY_4=0.8604
PRIMER_LEFT_4_PENALTY=0.275745
PRIMER_RIGHT_4_PENALTY=0.584629
PRIMER_LEFT_4_SEQUENCE=ATGAGCCTTCTCGATCACTTGT
PRIMER_RIGHT_4_SEQUENCE=GGTACCCAGCTGGTTTCACTAT
PRIMER_LEFT_4=39,22
PRIMER_RIGHT_4=252,22
PRIMER_LEFT_4_TM=60.276
PRIMER_RIGHT_4_TM=59.415
PRIMER_LEFT_4_GC_PERCENT=45.455
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=8.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=5.3000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_4=214
=
PRIMER_SEQUENCE_ID=SSR_06401 Ps_contig_mira-and-tgicl-ass_franssen_45625
SEQUENCE=TACGGCGTNGGGATCAACACAAACCTAAGCATTTCAACTATTTATATATATATTCACAGAGATCATATCGTATATCAATAAGATGACTTGGTTTATCAGACAACT
TARGET=43,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06402 Ps_contig_mira-and-tgicl-ass_franssen_45655
SEQUENCE=GCTGGGACCATTGATAAGAATACAATTATCAAAAGCATAACAATCTTTTCAATTTCATTGCAATATACACACACGATGGCCGCATCATCATCATCATCCATGGCTCTCTCTTCTCCAACCTTGGCTGGCAAGCAACTCAAGACTGACCCCACTCAAGCCAAGAATTGGGAGGCTGCAAGGTTCACCATGAGGAAGTCTGCTACCACCAAGAAAGTAGCTTCCTCTGGAAGCCCATGGTACGGACCAGACCGTGTTAAGTACTTAGGCCCATTCTCCGGTGAGTCTCCATCCTACNTGACTGGAGAGTTCCCCGGTGACTACGGTTGGGACACTGCCGGACTCTCTGCTGACCCAGAGACAATTACTCCAAGAACCGTGAGCTTGAAGTCATCCACT
TARGET=83,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_PAIR_PENALTY=0.7095
PRIMER_LEFT_PENALTY=0.627348
PRIMER_RIGHT_PENALTY=0.082156
PRIMER_LEFT_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_SEQUENCE=CTCCAGTCANGTAGGATGGAGA
PRIMER_LEFT=57,22
PRIMER_RIGHT=303,22
PRIMER_LEFT_TM=59.373
PRIMER_RIGHT_TM=59.918
PRIMER_LEFT_GC_PERCENT=40.909
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.50
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=1.50
PRIMER_LEFT_END_STABILITY=8.1000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_COMPL_ANY=3.75
PRIMER_PAIR_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=247
PRIMER_PAIR_PENALTY_1=0.8455
PRIMER_LEFT_1_PENALTY=0.627348
PRIMER_RIGHT_1_PENALTY=0.218111
PRIMER_LEFT_1_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_1_SEQUENCE=TCCAGTCANGTAGGATGGAGAC
PRIMER_LEFT_1=57,22
PRIMER_RIGHT_1=302,22
PRIMER_LEFT_1_TM=59.373
PRIMER_RIGHT_1_TM=59.782
PRIMER_LEFT_1_GC_PERCENT=40.909
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=8.1000
PRIMER_RIGHT_1_END_STABILITY=6.1000
PRIMER_PAIR_1_COMPL_ANY=3.75
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=246
PRIMER_PAIR_PENALTY_2=0.9293
PRIMER_LEFT_2_PENALTY=0.627348
PRIMER_RIGHT_2_PENALTY=0.301926
PRIMER_LEFT_2_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_2_SEQUENCE=TCTTGGTGGTAGCAGACTTCCT
PRIMER_LEFT_2=57,22
PRIMER_RIGHT_2=210,22
PRIMER_LEFT_2_TM=59.373
PRIMER_RIGHT_2_TM=60.302
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=154
PRIMER_PAIR_PENALTY_3=0.9293
PRIMER_LEFT_3_PENALTY=0.627348
PRIMER_RIGHT_3_PENALTY=0.301926
PRIMER_LEFT_3_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_3_SEQUENCE=CTTGGTGGTAGCAGACTTCCTC
PRIMER_LEFT_3=57,22
PRIMER_RIGHT_3=209,22
PRIMER_LEFT_3_TM=59.373
PRIMER_RIGHT_3_TM=60.302
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=54.545
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.1000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=153
PRIMER_PAIR_PENALTY_4=1.2164
PRIMER_LEFT_4_PENALTY=0.627348
PRIMER_RIGHT_4_PENALTY=0.589035
PRIMER_LEFT_4_SEQUENCE=TTGCAATATACACACACGATGG
PRIMER_RIGHT_4_SEQUENCE=GAGGAAGCTACTTTCTTGGTGG
PRIMER_LEFT_4=57,22
PRIMER_RIGHT_4=223,22
PRIMER_LEFT_4_TM=59.373
PRIMER_RIGHT_4_TM=59.411
PRIMER_LEFT_4_GC_PERCENT=40.909
PRIMER_RIGHT_4_GC_PERCENT=50.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=2.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.1000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=167
=
PRIMER_SEQUENCE_ID=SSR_06403 Ps_contig_mira-and-tgicl-ass_franssen_45660
SEQUENCE=TGATATCTTCAGGATCCACTAGTAGATTGTGATCACCATCTTTCCACCCAATAAGCTTTGGTGGAACCCCAACACCATTTGTGCAAAACAACCTTGCATTATCAACAAGTTTGCAATATTTTGGAAATGCCTTGGCAAATCTTTTGTGAGATTTCAATTGTGAATTGACCCTTACTGCCCTCCCTGTCATAATAGCTCTCCTAATGCCTCTAAATACGTCTAGGAACGAACCGACCGAACCAAACGGGGAGGGTAAGTACGGAACGGAACGGACCAACCGAACGGGG
TARGET=259,15
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
=
PRIMER_SEQUENCE_ID=SSR_06404 Ps_contig_mira-and-tgicl-ass_franssen_45668
SEQUENCE=TTCACTATAAGAGGAGTAATTGAAGTATATGAACCGATTAACATACGACACGAGTTGAGTGAGGGTGTGAATCCTTGCTAGTAATGACACAAGTATTCGCCTAATTAAGAAAAATAAAATGGAGAATGTTAGAGTAAGAAACATTAATTGAAACATGATCGAAAAACACATACATAAAGAGGTCACATTAGCTGCTAGTAAGCAGAGTCTTATTTAGAACTAGTAGGAAACTAGTAAGCATTATTCATACTTGTGAGACGATATTGCCAGTAGTTGCTTCCAAGTTTGATGTGAAGGACCATGAATGGATGTTGTGTGATACGGCAATTGTTGAAGTAGCAGAAAAACCAACGCTAACGTCCTTGGGGAGCACAGCTTTCAAATCAACGTTTTGTGAAATGGTAGAAATTTGACCATTCTCATAAGTGATAACAGCAGTTAAGGTGTTAGAAGGAGAGTCATAGATTATAGTTACATTAGTCAATGAACCACTCACAAAGTTATATCTGACGGTCTTGGTAGAAATTAAAGAGTTGATGTCGATTCCAATATGTCTCATATAGGGATCGAAAGAATTGGCATAAAGGTCAAACTCTAGACCAACGAATCGATTGATTGAAGTTTTACTATCAACTACTCCCAGATATTGACTTTGTGAGTTGTTGGGAATCTCAGTGTCCGGTGGTGCAATGAAAAATACGAGTCCATCAGTTGGAACACGTCCAGACGGGTTCAGTATGACGAAAGAAAAGGAAGTGACAAAACTGGCAACATTGCCGGTAGCACTGTCCCAAAGAGGCACGGGTGTTGAATATAAGGCACGACCTGTACTTGGGAAAGTTGTAGTTGGAGGAATTTGTGTACTGTTGGTCAGTGCCAAGACACCATTGGCTAAAATCTTGGCATTCCCTTGGAGGGTGATAGCAGTGTTACCAGGAGTGATCTTGGGGAAATTGAAGGAAAGTGCTTCGATATTTGTGGCTAGCAAAACAAACATGATTGAAACAAGGGAGACTAGTTCTTTAGTGCGATATAAAGCCATTCTAACTATTCTGAGATTTTGTTACCAAAGTTATCAATCNNNNNGTAYTTGGTTATCACACATATTTATATAGGCACTTGGGTGTACTTTTACCATCACATATCATTAAAATTATTTTACAACACCTGTTAAATATTATATGCACCAAAGTTTACTTTTGCTGGAATACTTGATGTGAATTTGAAAAACTCATCCCTATTAATTAATGAAAGTTAATTAGGTGAAAATTAATTAATTAATATTAATTAATAATTATAATGTGTCACTCATCAAAACCTAATTTTGAATGGGTTGTTTTATCTTGAAGATGTCGTCGAAACCCGACTGCATTGCATAATTATTGACAGTATGACGAAATATCTTAAAGAAAGTGATCTAGTACGCGACTCGTCCAGATAATTTTCTTTTACGAATTTTTTTTTATCAATATATAAAAGTAGAACAAAGCCGATCAAAACGTTCAGGGCGAGACGGAATAGATTTCTAGTTACTACGTATTTTAGTTTAAAAATAATTAAAAAATTATTTAAAAGTCCCCCTCACAAAATAAAATACTCACTTATATGTCATTATCTTATCATTTCATTTAAAAATAATCTTTACAAATAATAAATTTGACATTTAAAAAAAATGATCGGTTATTTTTCTCTTTAGATATGTTTTTCATTATTTATCTAAATTTTTTTAAAAATAATTAAGGATATTTTTGCCCCTATTTCACTATTTAAATATATTTTTATATATATATAGAATATAATAATAATTTCACACATTTAAAAAGAAAATAAAATCACATTTTATTTATTTAAATAATATAATTTTGTAAAAGAAATGAAGCACATGTAACGCGAGGAGGAAGCATACATGTTACTATATCATTATATCATTCTGATATTATTGTTGTGTCCAACTATGATAATATTATATAATTCTTTATTTTTTATTATAAATTGTTTTAGACTGTTCACACGTATTAAATTTATACATAGAAGACAGTGTTTTTTGAACATACAAGTCCACAACTCATTAAGGTGGCAATTATATGATTATGATATTATAGTATGTGTCAAACTATGTTTATATTATATTATCTTCCATTTTTTTTATTATAAATCGTTTTAAAATTTTCATACAGGTAAACCATGTTAATATTAATTTTGGAAAAGAAAGGAAGCGCATGTAACACGAGGAGGAAGGATTCATGTTACTATATCATTATTGTATGTGTCNAACTATGATAATATTATTTTATTGTTTATGTTTTATTATAAATTGTTTTAAACTTTTCACATGCACTCCCTCCGTTCCTTTATAAGTGTCACTTTCTTGCAAAAAAAAATTGTTTTNTTTTAATTGTCACTTGCAAAGTCCAAGGTAATATTAATTGCAATTTTGTCAAAATTATCTCTAGATAATGATTGGAGAGAGAGAAAAAGTAAAGTGAATGAATAAATAATTAAGGGTATTATAAGTAAAAGAAGAATTATTGTTTGAAAAATAACAATAATGATTAGCTTCCTTGATATGTGCAAAAATCAAAAAAATGACACTTAAAAAAGAACGGAGGGAGTTAATATTTTAGATTTTTACACGTATTAAAAAAATTATATCAAAAAAATATCTTTATATATTAATAAAATGGAGAAGATAAATTTATATAATTAAAAAAAAAGATAATAATAAAATTCTAGAGTTATAATAAAAAACTAATATTAATTCTTTTTAACATTGTAAAATGATTTATATTATGATATAATTTTTTTTCAAAACAACCATAATAAAAAATGATAGGGAGTATTATCATATGTCAGAAATTATTATAAAAGAAACAAGTTTGGTTTGGAAATGATACAAATTTGGTTTGGAAATGAAAGCTAGTATTCAATGTGTCAAACTAATTGGAGTGCCATAAAAGCTAGTATTCAATGTGTCCAACTAACTACTAATTCGAGTCGATATTAAAATATTAATTTATATTCTTTTTAGATTACTTTTTTAAATCTTATTTTTAATGACATGTTAGTAATAATGGTGATATGCCAGTATTAAATCAATATTAAAATATTAATTTATATCATTTTTTAGATTAATTTTTTTATAAAAANTTAATCATTTATTTTAAACAAATAAATGTTAATAAATATCACATTAATGTTTTTTTCTTCTTAGTCTGATCTTTAAAAAATATTTGGCATAATTTTTTTTTTAATATCTATAACAAATTTTTATAAAGAGTGATGTTATATTAACATCTAAAAACAATTATTTATTAATAATATTGTCATTGAATTAAAGCATATAGATTTAGAGTATTCAATATTATCATATTATAGATTAGGAGTCACCAAGACATGCTTATTAGAACTCCTTTATCTTCTTTGTTTCATGTTTATCTCCTCTTGCTTGATATATGGAAGATCTCCATATATTATTTCAAAAGTATCCCACATATCCTTGAAGATTTTACAACGATAAACATAATAAAATTTGCTATCATCTAGAGACATAGTTATAATTCTATTGGTTTTGAAATAAATTTCATATTTTATGTTTTCCTCTTCGGTCCAAAAGGAATCAGGTTTGTCCACTTCTTCATCATTTACTTTAAATATTGATAAAAATTAAAAAAATATACAAAATTATATTGTATATCTAATAGTATAGTGATTTATGAGATTAAGTTTTGATTTTTTTCGTTGCTTGTGGTATTCCAGAAAAATGTATCACGTATTTAGAAAAATGTATCTCATGTATTTATGGTAGGGTTAACAAAGGAAATAATTATTAAATATTTTTAAAATATGAAAATTCAATGGCGATAATTATAATTTACATAAGCTTGA
TARGET=1268,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06405 Ps_contig_mira-and-tgicl-ass_franssen_45668
SEQUENCE=TTCACTATAAGAGGAGTAATTGAAGTATATGAACCGATTAACATACGACACGAGTTGAGTGAGGGTGTGAATCCTTGCTAGTAATGACACAAGTATTCGCCTAATTAAGAAAAATAAAATGGAGAATGTTAGAGTAAGAAACATTAATTGAAACATGATCGAAAAACACATACATAAAGAGGTCACATTAGCTGCTAGTAAGCAGAGTCTTATTTAGAACTAGTAGGAAACTAGTAAGCATTATTCATACTTGTGAGACGATATTGCCAGTAGTTGCTTCCAAGTTTGATGTGAAGGACCATGAATGGATGTTGTGTGATACGGCAATTGTTGAAGTAGCAGAAAAACCAACGCTAACGTCCTTGGGGAGCACAGCTTTCAAATCAACGTTTTGTGAAATGGTAGAAATTTGACCATTCTCATAAGTGATAACAGCAGTTAAGGTGTTAGAAGGAGAGTCATAGATTATAGTTACATTAGTCAATGAACCACTCACAAAGTTATATCTGACGGTCTTGGTAGAAATTAAAGAGTTGATGTCGATTCCAATATGTCTCATATAGGGATCGAAAGAATTGGCATAAAGGTCAAACTCTAGACCAACGAATCGATTGATTGAAGTTTTACTATCAACTACTCCCAGATATTGACTTTGTGAGTTGTTGGGAATCTCAGTGTCCGGTGGTGCAATGAAAAATACGAGTCCATCAGTTGGAACACGTCCAGACGGGTTCAGTATGACGAAAGAAAAGGAAGTGACAAAACTGGCAACATTGCCGGTAGCACTGTCCCAAAGAGGCACGGGTGTTGAATATAAGGCACGACCTGTACTTGGGAAAGTTGTAGTTGGAGGAATTTGTGTACTGTTGGTCAGTGCCAAGACACCATTGGCTAAAATCTTGGCATTCCCTTGGAGGGTGATAGCAGTGTTACCAGGAGTGATCTTGGGGAAATTGAAGGAAAGTGCTTCGATATTTGTGGCTAGCAAAACAAACATGATTGAAACAAGGGAGACTAGTTCTTTAGTGCGATATAAAGCCATTCTAACTATTCTGAGATTTTGTTACCAAAGTTATCAATCNNNNNGTAYTTGGTTATCACACATATTTATATAGGCACTTGGGTGTACTTTTACCATCACATATCATTAAAATTATTTTACAACACCTGTTAAATATTATATGCACCAAAGTTTACTTTTGCTGGAATACTTGATGTGAATTTGAAAAACTCATCCCTATTAATTAATGAAAGTTAATTAGGTGAAAATTAATTAATTAATATTAATTAATAATTATAATGTGTCACTCATCAAAACCTAATTTTGAATGGGTTGTTTTATCTTGAAGATGTCGTCGAAACCCGACTGCATTGCATAATTATTGACAGTATGACGAAATATCTTAAAGAAAGTGATCTAGTACGCGACTCGTCCAGATAATTTTCTTTTACGAATTTTTTTTTATCAATATATAAAAGTAGAACAAAGCCGATCAAAACGTTCAGGGCGAGACGGAATAGATTTCTAGTTACTACGTATTTTAGTTTAAAAATAATTAAAAAATTATTTAAAAGTCCCCCTCACAAAATAAAATACTCACTTATATGTCATTATCTTATCATTTCATTTAAAAATAATCTTTACAAATAATAAATTTGACATTTAAAAAAAATGATCGGTTATTTTTCTCTTTAGATATGTTTTTCATTATTTATCTAAATTTTTTTAAAAATAATTAAGGATATTTTTGCCCCTATTTCACTATTTAAATATATTTTTATATATATATAGAATATAATAATAATTTCACACATTTAAAAAGAAAATAAAATCACATTTTATTTATTTAAATAATATAATTTTGTAAAAGAAATGAAGCACATGTAACGCGAGGAGGAAGCATACATGTTACTATATCATTATATCATTCTGATATTATTGTTGTGTCCAACTATGATAATATTATATAATTCTTTATTTTTTATTATAAATTGTTTTAGACTGTTCACACGTATTAAATTTATACATAGAAGACAGTGTTTTTTGAACATACAAGTCCACAACTCATTAAGGTGGCAATTATATGATTATGATATTATAGTATGTGTCAAACTATGTTTATATTATATTATCTTCCATTTTTTTTATTATAAATCGTTTTAAAATTTTCATACAGGTAAACCATGTTAATATTAATTTTGGAAAAGAAAGGAAGCGCATGTAACACGAGGAGGAAGGATTCATGTTACTATATCATTATTGTATGTGTCNAACTATGATAATATTATTTTATTGTTTATGTTTTATTATAAATTGTTTTAAACTTTTCACATGCACTCCCTCCGTTCCTTTATAAGTGTCACTTTCTTGCAAAAAAAAATTGTTTTNTTTTAATTGTCACTTGCAAAGTCCAAGGTAATATTAATTGCAATTTTGTCAAAATTATCTCTAGATAATGATTGGAGAGAGAGAAAAAGTAAAGTGAATGAATAAATAATTAAGGGTATTATAAGTAAAAGAAGAATTATTGTTTGAAAAATAACAATAATGATTAGCTTCCTTGATATGTGCAAAAATCAAAAAAATGACACTTAAAAAAGAACGGAGGGAGTTAATATTTTAGATTTTTACACGTATTAAAAAAATTATATCAAAAAAATATCTTTATATATTAATAAAATGGAGAAGATAAATTTATATAATTAAAAAAAAAGATAATAATAAAATTCTAGAGTTATAATAAAAAACTAATATTAATTCTTTTTAACATTGTAAAATGATTTATATTATGATATAATTTTTTTTCAAAACAACCATAATAAAAAATGATAGGGAGTATTATCATATGTCAGAAATTATTATAAAAGAAACAAGTTTGGTTTGGAAATGATACAAATTTGGTTTGGAAATGAAAGCTAGTATTCAATGTGTCAAACTAATTGGAGTGCCATAAAAGCTAGTATTCAATGTGTCCAACTAACTACTAATTCGAGTCGATATTAAAATATTAATTTATATTCTTTTTAGATTACTTTTTTAAATCTTATTTTTAATGACATGTTAGTAATAATGGTGATATGCCAGTATTAAATCAATATTAAAATATTAATTTATATCATTTTTTAGATTAATTTTTTTATAAAAANTTAATCATTTATTTTAAACAAATAAATGTTAATAAATATCACATTAATGTTTTTTTCTTCTTAGTCTGATCTTTAAAAAATATTTGGCATAATTTTTTTTTTAATATCTATAACAAATTTTTATAAAGAGTGATGTTATATTAACATCTAAAAACAATTATTTATTAATAATATTGTCATTGAATTAAAGCATATAGATTTAGAGTATTCAATATTATCATATTATAGATTAGGAGTCACCAAGACATGCTTATTAGAACTCCTTTATCTTCTTTGTTTCATGTTTATCTCCTCTTGCTTGATATATGGAAGATCTCCATATATTATTTCAAAAGTATCCCACATATCCTTGAAGATTTTACAACGATAAACATAATAAAATTTGCTATCATCTAGAGACATAGTTATAATTCTATTGGTTTTGAAATAAATTTCATATTTTATGTTTTCCTCTTCGGTCCAAAAGGAATCAGGTTTGTCCACTTCTTCATCATTTACTTTAAATATTGATAAAAATTAAAAAAATATACAAAATTATATTGTATATCTAATAGTATAGTGATTTATGAGATTAAGTTTTGATTTTTTTCGTTGCTTGTGGTATTCCAGAAAAATGTATCACGTATTTAGAAAAATGTATCTCATGTATTTATGGTAGGGTTAACAAAGGAAATAATTATTAAATATTTTTAAAATATGAAAATTCAATGGCGATAATTATAATTTACATAAGCTTGA
TARGET=1780,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06406 Ps_contig_mira-and-tgicl-ass_franssen_45668
SEQUENCE=TTCACTATAAGAGGAGTAATTGAAGTATATGAACCGATTAACATACGACACGAGTTGAGTGAGGGTGTGAATCCTTGCTAGTAATGACACAAGTATTCGCCTAATTAAGAAAAATAAAATGGAGAATGTTAGAGTAAGAAACATTAATTGAAACATGATCGAAAAACACATACATAAAGAGGTCACATTAGCTGCTAGTAAGCAGAGTCTTATTTAGAACTAGTAGGAAACTAGTAAGCATTATTCATACTTGTGAGACGATATTGCCAGTAGTTGCTTCCAAGTTTGATGTGAAGGACCATGAATGGATGTTGTGTGATACGGCAATTGTTGAAGTAGCAGAAAAACCAACGCTAACGTCCTTGGGGAGCACAGCTTTCAAATCAACGTTTTGTGAAATGGTAGAAATTTGACCATTCTCATAAGTGATAACAGCAGTTAAGGTGTTAGAAGGAGAGTCATAGATTATAGTTACATTAGTCAATGAACCACTCACAAAGTTATATCTGACGGTCTTGGTAGAAATTAAAGAGTTGATGTCGATTCCAATATGTCTCATATAGGGATCGAAAGAATTGGCATAAAGGTCAAACTCTAGACCAACGAATCGATTGATTGAAGTTTTACTATCAACTACTCCCAGATATTGACTTTGTGAGTTGTTGGGAATCTCAGTGTCCGGTGGTGCAATGAAAAATACGAGTCCATCAGTTGGAACACGTCCAGACGGGTTCAGTATGACGAAAGAAAAGGAAGTGACAAAACTGGCAACATTGCCGGTAGCACTGTCCCAAAGAGGCACGGGTGTTGAATATAAGGCACGACCTGTACTTGGGAAAGTTGTAGTTGGAGGAATTTGTGTACTGTTGGTCAGTGCCAAGACACCATTGGCTAAAATCTTGGCATTCCCTTGGAGGGTGATAGCAGTGTTACCAGGAGTGATCTTGGGGAAATTGAAGGAAAGTGCTTCGATATTTGTGGCTAGCAAAACAAACATGATTGAAACAAGGGAGACTAGTTCTTTAGTGCGATATAAAGCCATTCTAACTATTCTGAGATTTTGTTACCAAAGTTATCAATCNNNNNGTAYTTGGTTATCACACATATTTATATAGGCACTTGGGTGTACTTTTACCATCACATATCATTAAAATTATTTTACAACACCTGTTAAATATTATATGCACCAAAGTTTACTTTTGCTGGAATACTTGATGTGAATTTGAAAAACTCATCCCTATTAATTAATGAAAGTTAATTAGGTGAAAATTAATTAATTAATATTAATTAATAATTATAATGTGTCACTCATCAAAACCTAATTTTGAATGGGTTGTTTTATCTTGAAGATGTCGTCGAAACCCGACTGCATTGCATAATTATTGACAGTATGACGAAATATCTTAAAGAAAGTGATCTAGTACGCGACTCGTCCAGATAATTTTCTTTTACGAATTTTTTTTTATCAATATATAAAAGTAGAACAAAGCCGATCAAAACGTTCAGGGCGAGACGGAATAGATTTCTAGTTACTACGTATTTTAGTTTAAAAATAATTAAAAAATTATTTAAAAGTCCCCCTCACAAAATAAAATACTCACTTATATGTCATTATCTTATCATTTCATTTAAAAATAATCTTTACAAATAATAAATTTGACATTTAAAAAAAATGATCGGTTATTTTTCTCTTTAGATATGTTTTTCATTATTTATCTAAATTTTTTTAAAAATAATTAAGGATATTTTTGCCCCTATTTCACTATTTAAATATATTTTTATATATATATAGAATATAATAATAATTTCACACATTTAAAAAGAAAATAAAATCACATTTTATTTATTTAAATAATATAATTTTGTAAAAGAAATGAAGCACATGTAACGCGAGGAGGAAGCATACATGTTACTATATCATTATATCATTCTGATATTATTGTTGTGTCCAACTATGATAATATTATATAATTCTTTATTTTTTATTATAAATTGTTTTAGACTGTTCACACGTATTAAATTTATACATAGAAGACAGTGTTTTTTGAACATACAAGTCCACAACTCATTAAGGTGGCAATTATATGATTATGATATTATAGTATGTGTCAAACTATGTTTATATTATATTATCTTCCATTTTTTTTATTATAAATCGTTTTAAAATTTTCATACAGGTAAACCATGTTAATATTAATTTTGGAAAAGAAAGGAAGCGCATGTAACACGAGGAGGAAGGATTCATGTTACTATATCATTATTGTATGTGTCNAACTATGATAATATTATTTTATTGTTTATGTTTTATTATAAATTGTTTTAAACTTTTCACATGCACTCCCTCCGTTCCTTTATAAGTGTCACTTTCTTGCAAAAAAAAATTGTTTTNTTTTAATTGTCACTTGCAAAGTCCAAGGTAATATTAATTGCAATTTTGTCAAAATTATCTCTAGATAATGATTGGAGAGAGAGAAAAAGTAAAGTGAATGAATAAATAATTAAGGGTATTATAAGTAAAAGAAGAATTATTGTTTGAAAAATAACAATAATGATTAGCTTCCTTGATATGTGCAAAAATCAAAAAAATGACACTTAAAAAAGAACGGAGGGAGTTAATATTTTAGATTTTTACACGTATTAAAAAAATTATATCAAAAAAATATCTTTATATATTAATAAAATGGAGAAGATAAATTTATATAATTAAAAAAAAAGATAATAATAAAATTCTAGAGTTATAATAAAAAACTAATATTAATTCTTTTTAACATTGTAAAATGATTTATATTATGATATAATTTTTTTTCAAAACAACCATAATAAAAAATGATAGGGAGTATTATCATATGTCAGAAATTATTATAAAAGAAACAAGTTTGGTTTGGAAATGATACAAATTTGGTTTGGAAATGAAAGCTAGTATTCAATGTGTCAAACTAATTGGAGTGCCATAAAAGCTAGTATTCAATGTGTCCAACTAACTACTAATTCGAGTCGATATTAAAATATTAATTTATATTCTTTTTAGATTACTTTTTTAAATCTTATTTTTAATGACATGTTAGTAATAATGGTGATATGCCAGTATTAAATCAATATTAAAATATTAATTTATATCATTTTTTAGATTAATTTTTTTATAAAAANTTAATCATTTATTTTAAACAAATAAATGTTAATAAATATCACATTAATGTTTTTTTCTTCTTAGTCTGATCTTTAAAAAATATTTGGCATAATTTTTTTTTTAATATCTATAACAAATTTTTATAAAGAGTGATGTTATATTAACATCTAAAAACAATTATTTATTAATAATATTGTCATTGAATTAAAGCATATAGATTTAGAGTATTCAATATTATCATATTATAGATTAGGAGTCACCAAGACATGCTTATTAGAACTCCTTTATCTTCTTTGTTTCATGTTTATCTCCTCTTGCTTGATATATGGAAGATCTCCATATATTATTTCAAAAGTATCCCACATATCCTTGAAGATTTTACAACGATAAACATAATAAAATTTGCTATCATCTAGAGACATAGTTATAATTCTATTGGTTTTGAAATAAATTTCATATTTTATGTTTTCCTCTTCGGTCCAAAAGGAATCAGGTTTGTCCACTTCTTCATCATTTACTTTAAATATTGATAAAAATTAAAAAAATATACAAAATTATATTGTATATCTAATAGTATAGTGATTTATGAGATTAAGTTTTGATTTTTTTCGTTGCTTGTGGTATTCCAGAAAAATGTATCACGTATTTAGAAAAATGTATCTCATGTATTTATGGTAGGGTTAACAAAGGAAATAATTATTAAATATTTTTAAAATATGAAAATTCAATGGCGATAATTATAATTTACATAAGCTTGA
TARGET=1839,12
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=
PRIMER_SEQUENCE_ID=SSR_06407 Ps_contig_mira-and-tgicl-ass_franssen_45668
SEQUENCE=TTCACTATAAGAGGAGTAATTGAAGTATATGAACCGATTAACATACGACACGAGTTGAGTGAGGGTGTGAATCCTTGCTAGTAATGACACAAGTATTCGCCTAATTAAGAAAAATAAAATGGAGAATGTTAGAGTAAGAAACATTAATTGAAACATGATCGAAAAACACATACATAAAGAGGTCACATTAGCTGCTAGTAAGCAGAGTCTTATTTAGAACTAGTAGGAAACTAGTAAGCATTATTCATACTTGTGAGACGATATTGCCAGTAGTTGCTTCCAAGTTTGATGTGAAGGACCATGAATGGATGTTGTGTGATACGGCAATTGTTGAAGTAGCAGAAAAACCAACGCTAACGTCCTTGGGGAGCACAGCTTTCAAATCAACGTTTTGTGAAATGGTAGAAATTTGACCATTCTCATAAGTGATAACAGCAGTTAAGGTGTTAGAAGGAGAGTCATAGATTATAGTTACATTAGTCAATGAACCACTCACAAAGTTATATCTGACGGTCTTGGTAGAAATTAAAGAGTTGATGTCGATTCCAATATGTCTCATATAGGGATCGAAAGAATTGGCATAAAGGTCAAACTCTAGACCAACGAATCGATTGATTGAAGTTTTACTATCAACTACTCCCAGATATTGACTTTGTGAGTTGTTGGGAATCTCAGTGTCCGGTGGTGCAATGAAAAATACGAGTCCATCAGTTGGAACACGTCCAGACGGGTTCAGTATGACGAAAGAAAAGGAAGTGACAAAACTGGCAACATTGCCGGTAGCACTGTCCCAAAGAGGCACGGGTGTTGAATATAAGGCACGACCTGTACTTGGGAAAGTTGTAGTTGGAGGAATTTGTGTACTGTTGGTCAGTGCCAAGACACCATTGGCTAAAATCTTGGCATTCCCTTGGAGGGTGATAGCAGTGTTACCAGGAGTGATCTTGGGGAAATTGAAGGAAAGTGCTTCGATATTTGTGGCTAGCAAAACAAACATGATTGAAACAAGGGAGACTAGTTCTTTAGTGCGATATAAAGCCATTCTAACTATTCTGAGATTTTGTTACCAAAGTTATCAATCNNNNNGTAYTTGGTTATCACACATATTTATATAGGCACTTGGGTGTACTTTTACCATCACATATCATTAAAATTATTTTACAACACCTGTTAAATATTATATGCACCAAAGTTTACTTTTGCTGGAATACTTGATGTGAATTTGAAAAACTCATCCCTATTAATTAATGAAAGTTAATTAGGTGAAAATTAATTAATTAATATTAATTAATAATTATAATGTGTCACTCATCAAAACCTAATTTTGAATGGGTTGTTTTATCTTGAAGATGTCGTCGAAACCCGACTGCATTGCATAATTATTGACAGTATGACGAAATATCTTAAAGAAAGTGATCTAGTACGCGACTCGTCCAGATAATTTTCTTTTACGAATTTTTTTTTATCAATATATAAAAGTAGAACAAAGCCGATCAAAACGTTCAGGGCGAGACGGAATAGATTTCTAGTTACTACGTATTTTAGTTTAAAAATAATTAAAAAATTATTTAAAAGTCCCCCTCACAAAATAAAATACTCACTTATATGTCATTATCTTATCATTTCATTTAAAAATAATCTTTACAAATAATAAATTTGACATTTAAAAAAAATGATCGGTTATTTTTCTCTTTAGATATGTTTTTCATTATTTATCTAAATTTTTTTAAAAATAATTAAGGATATTTTTGCCCCTATTTCACTATTTAAATATATTTTTATATATATATAGAATATAATAATAATTTCACACATTTAAAAAGAAAATAAAATCACATTTTATTTATTTAAATAATATAATTTTGTAAAAGAAATGAAGCACATGTAACGCGAGGAGGAAGCATACATGTTACTATATCATTATATCATTCTGATATTATTGTTGTGTCCAACTATGATAATATTATATAATTCTTTATTTTTTATTATAAATTGTTTTAGACTGTTCACACGTATTAAATTTATACATAGAAGACAGTGTTTTTTGAACATACAAGTCCACAACTCATTAAGGTGGCAATTATATGATTATGATATTATAGTATGTGTCAAACTATGTTTATATTATATTATCTTCCATTTTTTTTATTATAAATCGTTTTAAAATTTTCATACAGGTAAACCATGTTAATATTAATTTTGGAAAAGAAAGGAAGCGCATGTAACACGAGGAGGAAGGATTCATGTTACTATATCATTATTGTATGTGTCNAACTATGATAATATTATTTTATTGTTTATGTTTTATTATAAATTGTTTTAAACTTTTCACATGCACTCCCTCCGTTCCTTTATAAGTGTCACTTTCTTGCAAAAAAAAATTGTTTTNTTTTAATTGTCACTTGCAAAGTCCAAGGTAATATTAATTGCAATTTTGTCAAAATTATCTCTAGATAATGATTGGAGAGAGAGAAAAAGTAAAGTGAATGAATAAATAATTAAGGGTATTATAAGTAAAAGAAGAATTATTGTTTGAAAAATAACAATAATGATTAGCTTCCTTGATATGTGCAAAAATCAAAAAAATGACACTTAAAAAAGAACGGAGGGAGTTAATATTTTAGATTTTTACACGTATTAAAAAAATTATATCAAAAAAATATCTTTATATATTAATAAAATGGAGAAGATAAATTTATATAATTAAAAAAAAAGATAATAATAAAATTCTAGAGTTATAATAAAAAACTAATATTAATTCTTTTTAACATTGTAAAATGATTTATATTATGATATAATTTTTTTTCAAAACAACCATAATAAAAAATGATAGGGAGTATTATCATATGTCAGAAATTATTATAAAAGAAACAAGTTTGGTTTGGAAATGATACAAATTTGGTTTGGAAATGAAAGCTAGTATTCAATGTGTCAAACTAATTGGAGTGCCATAAAAGCTAGTATTCAATGTGTCCAACTAACTACTAATTCGAGTCGATATTAAAATATTAATTTATATTCTTTTTAGATTACTTTTTTAAATCTTATTTTTAATGACATGTTAGTAATAATGGTGATATGCCAGTATTAAATCAATATTAAAATATTAATTTATATCATTTTTTAGATTAATTTTTTTATAAAAANTTAATCATTTATTTTAAACAAATAAATGTTAATAAATATCACATTAATGTTTTTTTCTTCTTAGTCTGATCTTTAAAAAATATTTGGCATAATTTTTTTTTTAATATCTATAACAAATTTTTATAAAGAGTGATGTTATATTAACATCTAAAAACAATTATTTATTAATAATATTGTCATTGAATTAAAGCATATAGATTTAGAGTATTCAATATTATCATATTATAGATTAGGAGTCACCAAGACATGCTTATTAGAACTCCTTTATCTTCTTTGTTTCATGTTTATCTCCTCTTGCTTGATATATGGAAGATCTCCATATATTATTTCAAAAGTATCCCACATATCCTTGAAGATTTTACAACGATAAACATAATAAAATTTGCTATCATCTAGAGACATAGTTATAATTCTATTGGTTTTGAAATAAATTTCATATTTTATGTTTTCCTCTTCGGTCCAAAAGGAATCAGGTTTGTCCACTTCTTCATCATTTACTTTAAATATTGATAAAAATTAAAAAAATATACAAAATTATATTGTATATCTAATAGTATAGTGATTTATGAGATTAAGTTTTGATTTTTTTCGTTGCTTGTGGTATTCCAGAAAAATGTATCACGTATTTAGAAAAATGTATCTCATGTATTTATGGTAGGGTTAACAAAGGAAATAATTATTAAATATTTTTAAAATATGAAAATTCAATGGCGATAATTATAATTTACATAAGCTTGA
TARGET=2464,10
PRIMER_MIN_SIZE=20
PRIMER_OPT_SIZE=22
PRIMER_MAX_SIZE=25
PRIMER_MIN_GC=40.0
PRIMER_OPT_GC=50.0
PRIMER_MAX_GC=60.0
PRIMER_NUM_NS_ACCEPTED=1
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_ERROR=Unrecognized base in input sequence
=